LOCUS       DASUFX010000001        14880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_138996,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000001 DASUFX010000000
VERSION     DASUFX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138996"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..347)
                     /locus_tag="VFV99_00005"
     CDS             complement(21..347)
                     /locus_tag="VFV99_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057699.1"
                     /translation="MRNLIVRLLLIGTATGAIVAACYFQPPEPRGFRPLPPTREAKPL
                     APKPEKTQPSVVQMDTEFAPQQEPPPAPPPTNMPTDAGVSDVVDLPPVPDAGGLDAPT
                     IKNAPR"
     gene            431..1249
                     /locus_tag="VFV99_00010"
     CDS             431..1249
                     /locus_tag="VFV99_00010"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5057700.1"
                     /translation="MLTALIAAAVGGWFSIMCLSVFAHRSIAHRAVTLHPVVAHVMRF
                     WLWFSTGTPTRRWVAVHRKHHVFTDAEGDPHSPVVFGLPRIFFLGYWYYRREAMKPET
                     IEQYGANCPNDWLERNVYEKHDNLGLILMLALDIAVFGIGPGLLAYGVQIIWVSLWAA
                     GVINGIGHAWGYRNFQTQEGSRNIVPVALLVSGEELHNNHHRWPRSAKFSMKWYEIDA
                     GWMLIRSLYTFGLASDIYVRNKRWKPSNEPLVDIDLADAAQLPEERAVDPSAAP"
     gene            complement(1246..2136)
                     /locus_tag="VFV99_00015"
     CDS             complement(1246..2136)
                     /locus_tag="VFV99_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5057701.1"
                     /translation="MRDNLSTMSRALAVLTACLLLLSRPAGADAIDQGVKEIQSKSQK
                     VRLAAALGLSKSKDARAVLAVSGALTNDADPTIRRVSALALEKMVDASTAEDARELAL
                     DALEKAARSDRDAKVKSTAESALKALAGLRRSSSSSKSSKPTVFVNVDSAVDQSNRAP
                     KDAPDRVVKIVRRGIEKTGYSTSWPGGLPTSKELTQSKSQAYIVAANVKKIDVTKGAR
                     QTTIACTVAIRVAPWSGKDGGEKWEANKAASASGSAKAMTGNSDRDVNAGMRDCLEAV
                     AEDVTNRQVVPFLKRLAATP"
     gene            2188..2883
                     /locus_tag="VFV99_00020"
     CDS             2188..2883
                     /locus_tag="VFV99_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5057702.1"
                     /translation="MGFLDSVRVLGAMARITAPSLVDIIRGSLDRESVDERARWFGQR
                     VVELLNVDLHASGADRVPTGRAYVYMSNHQSHLDIPMLYATLPSPTIRMLGKKELFQI
                     PLWGYGLRQAEFIEVDRSNHMRAVQSIEHAARLIRDGVSIYLAPEGTRSRDGRIGKLK
                     KGGFHLAIDTHTPIVPVAIRGTIDILPRGGKVMQTGKRVDVTIGAPISVEGRELADLM
                     AEVSEFFISHVER"
     gene            2956..5499
                     /locus_tag="VFV99_00025"
     CDS             2956..5499
                     /locus_tag="VFV99_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5057703.1"
                     /translation="MTWVVPLALGGLAILVMVTFTMLSARKRHRAVIAPAQVVGPRIL
                     VHDINDDRCTGCDACVAVCPTNVLDLVANKSRVLRFQDCIQCEACMFACPTEALVMFP
                     EGATPPPLKIPEIDENFQTAVPGQYLIGEVAGKPLVKNAANLGRAVIEHMLTTGVYPG
                     ALGGGAVDVAIIGSGPGGLSAALTCIQKGLSYVVLDKEQMIASTIARYPKGKLVMAEP
                     YDTENLSLLPVFDSSKEQLIPIWNELIERVRLQIKLGESVETVKRGAQGFEVRTTVAT
                     YQAQRVVLSIGTRGKPRTLQVPGENLPKVFSLLEDPDEWQGRNVLVVGGGDSACEAAL
                     ALADSGAKVTISYRGRGFNRAAPKNKQSIERYAAEGRIKAKLGSQVLEFDQDTVTLAL
                     ADNSQKRYPNDAAFVLIGADPPVAWLEKVGVRFVERPHQFQFGKSDDIVRRFVARAME
                     CPEDAARAAAQVLGGSTGVEPSRAAAAISLPMPMGGEKVSGPKKWLRSATSIFSTRGA
                     SQMTPLPQKAPVSTQAGRGKKLDGPMPLSEFAKRQKTSHSHHHTHTGHGRRDQLTAGE
                     RTRILRMLRDEGGRLADEESQVHIGAAPPMPANYDFDFDDEGAQVAAPPAPSVRPDVP
                     AKPALVVGLEKARKKRQTGGPQPAQPQPPRVPPAPSQPPPMRAKPATAPPPLPLRAVS
                     PPPPPVLPARSKPASMPPPARAPRVNDPSEPAPARSNPSPRRGLPTPFSADAPRSSLF
                     DEPTRLGDVDAHLLDQTRPKQSAPDAEYPPKFLPATTELAPPMHFDEDEATRLASPDG
                     KLPPRRQPPPPRGQAPAPRGTAKHGDNERTRAVDIRNDPSINDIDWDID"
     gene            5535..9017
                     /locus_tag="VFV99_00030"
     CDS             5535..9017
                     /locus_tag="VFV99_00030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057704.1"
                     /translation="MAGSSGTDKTIPASDPGTTAAGTDKTLPVSNLRETQSGTEKTLP
                     ASDVRSPPSGTERTIPASDVRLPSSDGTEKTIPADAGASDSESAAPATADQRYRLGTE
                     IGRGGMGRVVEAYDTYLGRTVALKEVLSTQSASLTKRFNREVRITARLEHASIVPLYD
                     GGLMADGRPFYVMRRVSGRPLDELIALGGTLDKRLALLPNVLAAIDAIGHAHQRGVIH
                     RDLKPANILVGDLGETVVIDWGLAKVIGEEELPPPSFEPYVPTAGDSLKTQVGSVFGT
                     PGFMSPEQARGDELDPRSDVFALGATLYQLLAGKPPVGGKSATEVIESTLSKKIAPVT
                     KLAPATPPELATIIAKALAFEARDRYADAGELAEDVRRFTTGQLVAAHRYTRWQRVAR
                     YARRHRAALSVAALAAVAVAGLSWFSVHRILKERDVANAARSEAEQQRSLAITKADEA
                     RQRADQLLLAHARSLVDTSPTEAIAALKHIESTSPQTLDGARAVARAALMRGVAWGVR
                     SLPGMTTSFEMTRDGKRLVQVNRDGKLQVVDLEQRKQTAVTDIGKGSRATWVDGDRKL
                     LIDRDGQPPALWNPASGGLEPLAIGELRDFEKTPDGAFVAYIDAGYQVGLLDVAKKTG
                     APLWTAGTAERSLAIAVDASWVAFGDRIDKKRSRLVVVDRSGKLVLERPGTVTMLAAS
                     PTGKLAASFFEDIVELRPGNPTVTKLPLDANEAKFVHFMAYRGDLLDLVAVRALLTWS
                     AGRLIRGDALADTVYSARSVAGDVIVAAANDNNVHLVRRGAHLVLSLTDAPEGMYRVA
                     VGQDSTRVAATAKDAILVWDVADLFPAIIDTPPGIFVANRRMLVPEMTSWFIFDLDTG
                     KRTPIKMTIDGIPNGYLPIADENRAAIVLQTAQGYKVVGIHGDGTSEVLVDDLGKGWV
                     SAVRGNAIVYSLGKGRVFGKVGSEPSRELVTVDGEVASLAGVGKLGYAALSTSGELVK
                     GTFGGASFARTRITDLDKSAFIVADPTGTVFIASDNRLLRWRTDVQELARFAVAIDTI
                     TASDVGLGIYITLVNKDVLFIPATGNLTPQRVPISTFSAITDDGKRLVGKAPNAVEIV
                     EMPALAAWTLPKLIGGIGNPIESPDGARVLQNLGVQSAVWRIPEPGSDFAAWLAETTN
                     ATEDAGHVRWPWSP"
     gene            complement(9058..9774)
                     /locus_tag="VFV99_00035"
     CDS             complement(9058..9774)
                     /locus_tag="VFV99_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5057705.1"
                     /translation="MVDLIHRLRRVAQMAGDARVTSLEDAIRADARGGRALLEQRDAR
                     EVAIALMRLCDALGFESVTHARRLVAWCRILGEAASLDASELDTLVLGAQVHDIGKLG
                     VAANVLGADDALTATELDWIRMHSSLGHALIAGVAGLTGARELVLCHHEYWDGSGYPD
                     GRRGAATPRAARIFVLVDSYEAMIRDDVGYRASRNHDAARAEIIELAGVRYDPELVAI
                     WRAIDERRWASVASDIQLAA"
     gene            9933..10541
                     /locus_tag="VFV99_00040"
     CDS             9933..10541
                     /locus_tag="VFV99_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5057706.1"
                     /translation="MKRELLVRAPLFAAMPEKVVDGLAARASRRRLRKGQRVLTRGEA
                     AVIVVLTGRLEVVSDTGDEVALVRSLVSPAVVGVSVAVGAAASAELWVAEDAELAVIP
                     ADAVAAALRRYPEAAIAAIAHLGGVIAELSAEVAALRVHGLVERVRHKLRQLAKGRRE
                     LTITHARLADEVGGTRANVSRALARLEREGAIRRRRGRIELL"
     gene            10599..11741
                     /locus_tag="VFV99_00045"
     CDS             10599..11741
                     /locus_tag="VFV99_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEX5057707.1"
                     /translation="MLATQIRQKDSVFYFCAYPAEDLLDKVRFISRFYAEGAPSLAGE
                     GAEEGDEVATFISKIEATDAAFQRQLSRGKVTAIKNFYETAVSQPPIPGTVLLFTEEK
                     LKFEGVKPYTNVGNLQEPRGKYLIIDGQHRLAALHFFMHHRPDDAKTIHVPCVIFDGK
                     SEDFATEMFVIINSTPTRINKSHLIDLYEKVSWAAPDKKFAARVVDHLYREGDSPLRY
                     KINRLGGRSKQEKWILQAELFNEIHRWATSEWKQIKKSSSWQRAAERYYEMVRDFLRA
                     AEKAWGEGVWGHPSYMVTRPVTIKAMLRVCADLAAADPDPDDRVKRWARKLSPWGDFA
                     KSFRQEGFYERFPAKGQIERVARIHKELARAANIERRQKDKGEDEE"
     gene            11867..12913
                     /locus_tag="VFV99_00050"
     CDS             11867..12913
                     /locus_tag="VFV99_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5057708.1"
                     /translation="MAGEHIYFTWPDRRLRYDCRGCGACCKGLGVGIDVVAGQLVQLV
                     TRRPELAAFVRRRGDAVTAFNPRDRCWFLADDGLCRVEVEDGRAAKPASCRLFPFNRV
                     FRAGAYTIVDYNSVICPLAVVASDGVSHAEVLAEIATIQDPAIVGTQLPPDDALFARE
                     QAYAADLLARGADAIVADAVVNAAFDTILGTPWRAPAAPTLANALMLTPSLRFNELYG
                     PRQYAPRDRMQSLLTRMQLAWLGFAALGEQLAQRTLGLQELTTLWSEQAPLMHAIARW
                     DAVPVFKPGPVELPSVDPHNLVRTLGQRCVDNRNAKHTLGALVADVLTVADPATRVAA
                     LKMADPILRAVAWS"
     gene            12913..13764
                     /locus_tag="VFV99_00055"
     CDS             12913..13764
                     /locus_tag="VFV99_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057709.1"
                     /translation="MRGSVAMLVVSGACALVGGCDVVFRLDHVGSHGDGGVTGDVDDA
                     MTVDAPPSNTCMLSPSVFVPEITANYEDPAQSGAKLELFLSLYDTGVALADIYVARRE
                     AVSDPWGVPLVENGLSSTADDTDPVLAQNDTLALWTSTRVASTRKIFQAMRDGPNRPF
                     NTPMIVFDSTVSGIDVSPDGLTLYVCDGNGVMGQISRAATTVPWLPTAATVVGNGIYF
                     PSVAGDGLTVYYHRNTTLYRQTRMAPSGPFSNEVAIAAGTDANISDDGRELLYFTDTS
                     LARATCQ"
     gene            complement(13777..>14880)
                     /locus_tag="VFV99_00060"
     CDS             complement(13777..>14880)
                     /locus_tag="VFV99_00060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX5057710.1"
                     /translation="AVWRNYLVARTLLSTASYLTKDLEEARFKYTQSLTGQPEQEPRW
                     KRCIGRTDAALGDLLGQVFVRDRFPGASKSAAEEQVRAITSAMQANLDVLPWMDATTK
                     TRAREKLGAMANYIGYPAKWHEYKFKIDAKTWAANALAGRKAENARQIAKIGKPVDRD
                     DWGFSAPTVNAFYSPNQNKMVFPAGILQPPFYSVEFSIPVNLGGMGTVVGHELTHGFD
                     DQGAQYDAQGNMKNWWQPETEKQFKQRTQCVIDQYSKYGLNGANTVGENIADIGGVKL
                     SLAGYRALRASAPETVVADGFTEDQQFFLAFGQVWCEKMRPEFEKMLATVDVHSSGRW
                     RVNGTLSDTPDFAKAFRCKAGAKMRPANACVVW"
BASE COUNT         2456 a         5008 c         4953 g         2463 t
ORIGIN      
        1 cacgcaaagc gtgcacaccg ttaacgcggc gcgttcttga tcgtcggcgc gtcgaggcca
       61 cctgcatccg ggaccggcgg taagtcgacc acgtcagaca cgccggcatc cgtcggcatg
      121 ttcgtcggtg gcggtgcggg cggcggttcc tgttgcgggg cgaactcggt atccatctgg
      181 acgacgctcg gctgtgtctt ctcgggcttg ggcgcgagcg gctttgcttc ccgcgtcggc
      241 ggcaacggcc tgaacccacg cggctcgggc ggctggaagt aacaagcggc gacgatcgcg
      301 cccgttgcag taccgatgag gagcagtcgc acgatcaggt tccgcatgat cgggagcatt
      361 gccagccgcg tgcctcgtgc agtccgtgcg ctgccgctct cagccggcgg attagatgct
      421 atagagccgt gtgctcacag ccctgatcgc cgcggcagta ggcggatggt tctcgatcat
      481 gtgcctcagc gtgttcgcgc atcgctcgat cgcgcaccgc gctgtcacgc tgcacccagt
      541 agtcgctcat gtgatgcgct tctggctgtg gttctcgacc ggcacgccaa cccgacggtg
      601 ggtcgccgtc catcgcaagc atcacgtgtt caccgacgcc gaaggcgatc cgcactcgcc
      661 agtcgtgttc ggtctgccgc gcatcttctt cctcggctac tggtactacc gccgcgaggc
      721 gatgaagccg gagacgatcg agcagtacgg cgcgaactgt ccgaacgatt ggctcgagcg
      781 caacgtctac gagaagcacg acaacctcgg cctcatcctc atgctcgcgc tcgacatcgc
      841 ggtgttcggc atcggcccgg gcctgctcgc gtacggcgtg cagatcatct gggtgtcgct
      901 gtgggctgcc ggcgtgatca atggcatcgg tcacgcgtgg ggctatcgca acttccagac
      961 ccaagaaggc agccgcaaca tcgtgccggt cgcgctgctc gtgagcggcg aagagctgca
     1021 caacaatcat catcgctggc cgcggtcggc gaagttctcg atgaagtggt acgagatcga
     1081 cgcgggctgg atgctgatcc gctcgctgta cacgttcggt ctcgcgagcg acatctacgt
     1141 gcgcaacaag cgctggaaac catcgaacga gccgctcgtc gatatcgacc ttgcagatgc
     1201 ggcgcaactc cctgaagagc gtgctgtcga tccgtcggcc gcgccctaag gcgtcgcggc
     1261 gagccgcttc aagaatggca cgacctgccg gttcgtgacg tcttcggcga ccgcctcgag
     1321 acagtcgcgc atgccggcgt tgacgtcgcg atccgaattg ccggtcatcg ctttcgcaga
     1381 gccggatgcc gacgcggcct tgttcgcttc ccacttctcg ccgccgtcct taccgctcca
     1441 cggcgcgacg cgaatcgcga ccgtgcacgc gatggtggtc tggcgcgcgc ccttcgtgac
     1501 atcgatcttc ttcacgttcg ccgcgacgat gtacgcctgc gacttgctct gcgtgagctc
     1561 cttgctggtc ggcagtccgc cgggccagct cgtcgagtaa ccggtcttct cgatgccgcg
     1621 cctcacgatc ttgaccacgc gatcgggcgc atccttcggt gctcggttcg attgatcgac
     1681 cgcactatcg acgttgacga acacggtggg cttgctgctc ttcgagctcg acgagctacg
     1741 ccgtagtccg gcgagtgcct tgagtgcgct ctccgcggtc gacttcacct tcgcatcgcg
     1801 atcgctgcgc gcggcttttt cgagtgcatc gagcgcgagc tcgcgtgcat cttcggccgt
     1861 cgatgcatcg accatctttt cgagcgcgag tgcggacacg cgacgaatcg tcgggtcggc
     1921 atcgttcgtc agcgcgcccg agacggcgag cactgcgcgt gcgtctttcg atttcgagag
     1981 cccgagtgca gcagcgagtc gcaccttctg actcttcgac tgaatttctt tgacgccttg
     2041 atcgatcgcg tccgcacctg ctggtcgaga cagcagtagc aagcaagcgg tgagaaccgc
     2101 cagcgcgcgc gacatcgtgc tcaggttatc acgcacaaat tagaacgtag aggcccacac
     2161 atcccacctt cgcgtagact ctggcccgtg ggattcctcg acagtgttcg cgtgctcggt
     2221 gcgatggcgc gcatcaccgc gccatcactc gtcgacatca tccgcggctc gctcgatcgc
     2281 gagtcagtcg acgagcgagc tcgatggttt ggccagcgcg tggtcgagtt actgaatgtc
     2341 gatctccacg caagcggtgc ggaccgcgtg cccacgggtc gcgcgtatgt ctacatgtcg
     2401 aaccaccaga gccacctcga catcccgatg ctctacgcga cgctgccctc gccgacgatc
     2461 cgcatgctcg gcaagaagga gctgttccag atcccgctct ggggatacgg cctacgccag
     2521 gccgagttca tcgaagtcga tcgcagcaac cacatgcgcg cggtgcagtc gatcgagcac
     2581 gcggcgcggc tgatccgaga cggcgtcagt atctacttgg ctccggaggg gacgcgctcg
     2641 cgcgatggcc ggatcggcaa gctcaagaag ggcgggttcc atctcgcgat cgacacgcac
     2701 accccgatcg tgccggtggc tatccgaggc acgattgaca ttctgccgcg tggcgggaag
     2761 gtgatgcaga cgggcaaacg cgtcgatgtc acgatcggcg caccgatttc cgtcgagggc
     2821 cgcgagctcg ccgatctgat ggccgaggtc tcagaattct tcatcagcca cgtcgagagg
     2881 tagcggagac tacggagttt tcttggcgac ccgcgcgatg cgttatcgcc cgcatttata
     2941 gggtatcttt ctcgggtgac ctgggtcgtg ccgttggcgc tcgggggcct cgccatcctc
     3001 gtgatggtga cgttcacgat gctctccgcc cgcaagcgcc atcgcgccgt gatcgctccg
     3061 gctcaagtgg tcgggccgcg cattctcgtt cacgacatca atgatgatcg ctgcacgggg
     3121 tgcgacgcct gcgtcgccgt ctgcccgacc aacgttctcg atctggtcgc gaacaagagc
     3181 cgcgtgctgc ggttccagga ctgcatccag tgcgaagcgt gcatgttcgc gtgcccgacc
     3241 gaagcgctcg tgatgttccc ggaaggggcc acgccgccgc cgctcaagat tcccgagatc
     3301 gacgagaact tccagacggc cgtcccgggc cagtacctga tcggtgaagt cgctggcaag
     3361 ccgctcgtca agaacgcggc gaacctcggc cgcgcggtca tcgagcacat gctgacgacc
     3421 ggcgtgtatc cgggcgcgct cggtggcggt gcagtcgatg tcgcgatcat cggctcgggc
     3481 ccaggtggcc tgtctgccgc gctcacgtgc atccagaagg gcctgtcgta cgtcgtgctc
     3541 gacaaggagc agatgatcgc gtcgacgatc gcgcgctacc ccaagggcaa gctcgtgatg
     3601 gccgagccgt acgacaccga gaacctgtcg ctcttgcccg tgttcgactc gtcgaaggaa
     3661 cagctgattc cgatctggaa tgagctgatc gagcgcgtgc ggctgcagat caagctcggc
     3721 gaatcggtcg aaaccgtgaa gcggggcgcg caggggttcg aagtgcgcac gaccgtcgcg
     3781 acgtaccagg cgcagcgcgt cgtgctgtcg atcggtacgc gcggcaagcc gcgtacgctg
     3841 caggtgccgg gcgagaacct cccgaaggtg ttcagcctgc tcgaggatcc cgacgagtgg
     3901 caaggacgca acgtgctggt cgtcggtggc ggtgactccg catgtgaagc ggcgctcgcg
     3961 ctcgccgact ccggcgcgaa ggtcacgatt tcgtaccgcg gccgcggctt caatcgcgcg
     4021 gccccgaaga acaagcaatc gatcgagcgc tacgcggcgg aaggccgcat caaggcgaag
     4081 ctcggctcgc aggtcctgga gttcgatcaa gatacggtga cgctcgcact cgccgacaac
     4141 tcgcagaaac gctatccaaa cgacgccgcg ttcgtgctca tcggcgccga tccgccggtc
     4201 gcctggctcg agaaggtcgg cgttcgcttc gtcgagcgtc cgcaccagtt ccagttcggc
     4261 aagtcggatg acatcgtgcg ccgcttcgtc gcgcgcgcga tggaatgccc cgaggacgcg
     4321 gcacgtgcag cggcacaggt gctcggcggc agcactggcg tcgagccgtc gcgcgctgcg
     4381 gctgccatct cgctgccgat gccgatgggc ggcgagaagg tgagcggccc gaagaagtgg
     4441 ctgcgttcgg cgaccagcat cttctcgacg cgcggggcgt ctcagatgac gccgctgccg
     4501 cagaaggcac ccgtctcgac tcaggcgggg cgcggcaaga agctcgacgg tccgatgccg
     4561 ctgtcggagt tcgcgaaacg gcagaagacg tcgcactcgc atcaccacac gcacaccggt
     4621 catggtcgtc gcgatcagct gaccgccggc gaacgtacgc gcatcctccg catgctgcgc
     4681 gacgaaggcg gccgtctcgc cgacgaagaa tcgcaggtgc atatcggcgc cgcgccgccg
     4741 atgcccgcca actacgactt cgacttcgac gacgaaggcg cgcaggtcgc ggcaccgccg
     4801 gccccgtccg tgcggcccga cgttccggcg aagcctgcgc tcgtcgttgg cctcgagaag
     4861 gcgcgcaaga agcgccagac cggtggaccg cagccggcgc aaccgcagcc gccgcgcgtg
     4921 ccgcctgcac cgtcgcaacc accgcccatg cgtgcgaagc cggcgaccgc gccgccaccg
     4981 ctgccgctgc gtgcggtttc gccgccgccg ccgcccgtgc ttcctgcgcg gtcgaagccc
     5041 gcgtcgatgc cgccgccagc gcgcgcgccc cgtgtcaacg atccgagcga gccggcgccg
     5101 gcgcgatcga atccatcgcc acgtcgcggt ctgccgacgc cgttctcggc agacgcaccg
     5161 cggtcgtcgc tgttcgacga gccgacgcgg ctcggcgacg tcgatgcgca ccttctcgat
     5221 cagacgcggc cgaaacagtc cgcacccgac gccgagtacc cgccgaagtt cctgccggca
     5281 acgaccgagc tcgcgccgcc gatgcacttc gacgaggacg aagcgacgcg cctcgccagc
     5341 cccgacggca agctgccgcc gcgccgccaa ccaccgccac cgcgcggaca ggcgcccgcg
     5401 ccgcgtggca ctgcgaagca cggcgacaac gagcgcacgc gtgcggtcga cattcggaac
     5461 gaccccagca tcaacgacat cgactgggat atcgactgac cctcctcgtc gatatcgact
     5521 gatagactct gggcatggcc gggtcgtcgg ggaccgacaa aacgattccc gcgagtgatc
     5581 cggggacgac ggccgcaggc accgacaaga cgcttccggt gagcaatctg cgcgagacgc
     5641 agtcgggaac cgagaagacg ctgccggcga gcgacgtcag gagtccacca tcggggaccg
     5701 aacggacgat tcccgcgagt gacgtgcggc tgccgtcctc ggacggaacc gagaagacga
     5761 tccccgcgga cgccggcgcc tccgacagcg aatcggcagc gcccgcgacg gccgatcagc
     5821 ggtatcgcct cggcaccgag atcggacgcg gcggtatggg gcgcgtcgtc gaagcgtacg
     5881 acacgtacct cggccgcacg gtcgcgctga aggaagtgct gtcgacgcag agcgcgtcgc
     5941 tgacgaagcg gttcaaccgc gaagtgcgaa tcactgcgcg cctcgagcac gcgtcgatcg
     6001 tcccgctcta cgacggcggc ctgatggccg acggtcgccc gttctacgtg atgcgtcgcg
     6061 tgtcgggccg tccactcgac gagctgatcg cgctgggcgg tacgctcgac aagcggctcg
     6121 cgctgctgcc gaacgtgctc gctgcgatcg atgccatcgg gcacgcgcac cagcgcggcg
     6181 tcattcatcg cgatctcaag ccggcgaaca ttctcgtcgg tgatctcggc gagacggtgg
     6241 tgatcgactg gggccttgca aaggtgatcg gcgaggaaga gctgccgccg ccgagcttcg
     6301 agccgtacgt gccgaccgct ggcgacagct tgaagacgca ggtcggctcg gtgttcggga
     6361 cgccgggatt catgtcgccc gagcaagcgc gtggcgacga gctcgatccg cgcagcgacg
     6421 tgttcgcgct cggcgcgacg ctctaccaac tgctcgcggg caagccgccg gtcggcggca
     6481 agagcgcgac cgaggtgatc gaatcgacgc tgtcgaagaa gatcgcgcca gtcacgaagc
     6541 tggcaccggc gacgccgccc gagctcgcga cgatcatcgc gaaggcgctc gcgttcgaag
     6601 cgcgtgaccg ctacgccgat gccggcgagc tcgccgagga cgtgcgtcgg ttcacgaccg
     6661 gtcagctcgt cgcggcgcat cgctacacgc gctggcagcg agtcgctcgc tatgcacggc
     6721 gtcatcgcgc tgcgctgtcg gttgctgcgc ttgccgcggt cgcggtcgcg gggctgtcgt
     6781 ggttcagcgt gcatcgcatc ctgaaagagc gcgatgtcgc gaacgccgcg cgcagcgaag
     6841 ccgagcaaca acgctcgctc gcgatcacga aggcggacga agcgcgccag cgtgcggatc
     6901 agctgttgct cgcgcacgcg cgcagcctcg tcgacacgag cccgacagaa gcgattgccg
     6961 cgctcaaaca catcgagtcg acctcgccgc agacgctgga tggtgcgcgt gcggtcgcgc
     7021 gcgcggcgct gatgcgcggt gtcgcctggg gcgtgcgctc gctgcccggg atgacgacga
     7081 gcttcgagat gacccgcgat gggaagcggc tcgtccaggt caatcgcgac ggcaagctcc
     7141 aggtcgtcga cctcgagcag cgtaagcaga ccgcggtgac cgacatcggc aaaggcagcc
     7201 gcgcgacctg ggtcgacggc gatcgcaagc tgctgatcga ccgcgacggc caaccgcccg
     7261 cgctgtggaa tccggcgagc ggcgggctcg aaccgctcgc gatcggcgag ctgcgcgact
     7321 tcgagaagac gcccgatggc gcgttcgtcg cgtatatcga tgcgggctat caagtcggtc
     7381 tcctcgacgt ggcgaagaag accggcgcgc cactgtggac cgccggaacc gcagagcgca
     7441 gcctcgcgat cgcggtcgat gcgagctggg tcgcgttcgg cgatcgcatc gacaagaagc
     7501 gctcgcgcct cgtcgtcgtc gatcgcagtg gcaagctcgt gctggagcgg ccgggcactg
     7561 tgacgatgct cgccgcttcg ccgaccggca agctcgcagc ctcgttcttc gaggacatcg
     7621 tcgagctccg tccgggcaac ccgacggtga ccaagctgcc gctcgacgcg aacgaggcga
     7681 agttcgtcca cttcatggcg tatcgcggtg acctgctcga cctcgtcgcc gtgcgcgcgc
     7741 tgctcacgtg gagcgccggt cgcttgatcc gtggtgacgc gctcgcagat accgtttact
     7801 cggcgcggtc agtcgccggt gatgtgatcg tcgctgccgc gaacgacaac aacgtccacc
     7861 tcgtgcgacg cggggcacac ctggtgctgt cgttgaccga cgcgcccgaa ggcatgtacc
     7921 gcgtcgcggt tgggcaggac agtacccgcg tcgcggcgac ggcgaaggac gcgatcctgg
     7981 tctgggatgt cgccgatctg ttccccgcga tcatcgatac gccgccgggt atcttcgtcg
     8041 cgaaccgccg catgctcgtt cccgagatga cgagctggtt catcttcgat ctcgataccg
     8101 gcaagcgcac gccgatcaag atgacgatcg acggcatccc gaacggctac ttgccgatcg
     8161 ccgacgagaa ccgcgccgcg atcgttctgc agacggcgca gggctacaag gtcgtcggca
     8221 tccacggcga cggtacgagc gaggtgctcg tcgacgatct cggcaagggc tgggtcagcg
     8281 ccgtgcgggg caacgccatt gtctattcgc tcggcaaggg ccgcgtgttc ggcaaggtcg
     8341 gtagcgaacc gtcgcgcgag ctcgtcaccg tcgacggtga ggtcgcgtcg ctcgcgggtg
     8401 tcggcaagct cggctacgcc gcgctcagca cgagcggcga gctggtgaag ggcacgttcg
     8461 gcggcgcgag tttcgcgcgc acgcgcatca ccgatctcga caagtcggcg ttcatcgtcg
     8521 cggatccaac aggcaccgtg ttcatcgcca gcgacaaccg gctgctgcgc tggcgcactg
     8581 acgtccagga gctcgcgcgg ttcgcggtcg cgatcgatac catcactgcc tctgacgtcg
     8641 gcctcggcat ctacataacg ctggtcaaca aggacgttct gtttataccg gcgactggca
     8701 acctgacgcc gcaacgcgtg ccgatctcca cgttcagcgc gatcaccgac gacggcaagc
     8761 gtctcgtcgg caaggcaccg aacgcagtcg agatcgtcga gatgccggca ctcgctgcgt
     8821 ggacactgcc gaagctgatc ggcggcatcg gtaatccgat cgaatcaccc gacggtgcac
     8881 gcgtgctcca gaacctcggc gtgcagtccg cggtctggcg catcccggag ccgggctccg
     8941 acttcgcggc atggctcgcc gagaccacga acgcgaccga ggacgccggc cacgtgcgct
     9001 ggccgtggtc gccttgacag gcttcgggcc tcgggcttcg gacttcggcc agaacgctca
     9061 tgccgcgagc tggatgtcgc tcgcgaccga cgcccagcgg cgctcgtcga tggcgcgcca
     9121 gatcgcgacg agctccgggt cgtagcgaac gcccgccagc tcgatgatct cggcgcgcgc
     9181 ggcatcgtga ttgcggctcg cgcgatagcc gacgtcgtcg cggatcatcg cctcgtacga
     9241 gtcgacgagc acgaagatgc gcgctgcacg cggcgtcgct gcgccgcgcc gaccgtccgg
     9301 gtagccactg ccatcccagt actcgtggtg acagagcacg agctcgcgcg caccggtgag
     9361 gccagcgacg ccggcgatca gcgcgtgacc gagcgagctg tgcatgcgaa tccagtcgag
     9421 ctcggtcgcg gtgagcgcgt catcggcgcc gagcacgttg gccgcgacgc cgagcttgcc
     9481 gatgtcgtgg acctgcgcgc cgagtacgag cgtgtcgagc tcgctggcgt cgaggctggc
     9541 cgcctcgccg aggatgcggc accacgcgac gagccggcgt gcatgggtga cgctctcgaa
     9601 gccgagcgca tcgcacagcc gcatcagtgc gatcgcgacc tcgcgtgcgt cgcgctgttc
     9661 gagcaacgcg cggccgcccc gcgcatcggc acgaatggcg tcctcgagcg acgtcacgcg
     9721 cgcgtcgccc gccatctgcg cgacgcgccg caatcggtga atcagatcga ccatcgtagt
     9781 cctcctgcac gcagcctgct gccggtgcgg tcgggccatg gtgtcgatcg acaccgaaga
     9841 ggttgaaccg ccagggcgcc gcagacgcca agccccagaa aaccgatctt ggaccttggc
     9901 gttcgtgggc gtcttggcgg ttactccggt gtgtgaagcg cgagctgctt gtccgcgcgc
     9961 cgttgttcgc ggcgatgccc gagaaggtgg tcgatgggct tgcggcgcgg gcgagccgcc
    10021 gtcgactacg caagggccag cgtgtgctga cccgcggcga ggcagcggtc atcgtcgtcc
    10081 tcacgggccg gctcgaggtg gtgagcgaca ccggagacga agtcgcgctc gtgcgatcgc
    10141 tcgtgtcgcc cgcggtcgtc ggagtctcgg tggcggtcgg cgccgcagcg agcgccgagc
    10201 tgtgggttgc agaggacgcg gagctggcgg tcatcccggc cgatgcggtg gcggcagcgc
    10261 tgcggcgcta tcccgaggcg gcgatcgccg cgatcgcgca cctcggcggt gtgatcgccg
    10321 agctctcggc cgaggtcgcc gcattacgcg tgcacggcct cgtggagcgg gtccgccaca
    10381 agttgcggca gctggccaaa ggccgccgcg agctgacgat cacccacgcg cgacttgcgg
    10441 acgaggtggg cgggacccgt gccaacgtgt cccgtgcact ggcccggctc gagcgcgagg
    10501 gggctattcg ccgccgtcgg ggccgaatcg agctgctctg agtgtagaga tcacccttag
    10561 cgacgcttgc cgtcaccggg ctgggctagg atgcgtccgt gctagcgacc cagatcaggc
    10621 aaaaggacag cgtcttctac ttctgcgcgt atccggccga ggacctgctc gacaaggtcc
    10681 gcttcatcag ccggttctac gcggaaggtg cgcccagcct cgcgggcgaa ggtgcagagg
    10741 aaggggacga agtcgcgacg ttcatctcga agatcgaggc gaccgacgcc gcgttccagc
    10801 gtcagctctc gcgcggcaag gtcaccgcga tcaagaactt ctacgagaca gcggtcagcc
    10861 agccgccgat tcccggcacg gtgctgctgt tcaccgaaga gaagctcaag ttcgagggcg
    10921 tcaagccgta cacgaacgtt ggcaacctgc aggagccgcg cggcaagtac ctcatcatcg
    10981 acggccagca ccgcctcgcg gcgctgcact tcttcatgca ccaccgcccg gatgacgcga
    11041 agacgatcca cgtcccgtgc gtgatcttcg acggcaagag cgaagacttc gcgaccgaga
    11101 tgttcgtcat catcaactcg acgccgacgc gcatcaacaa atcgcacctc atcgacctct
    11161 acgagaaggt gtcgtgggct gcgcccgaca agaagttcgc cgcgcgcgtc gtcgatcatc
    11221 tgtaccgcga aggcgacagc ccgctgcgct acaagatcaa ccgcctcggc ggccgcagca
    11281 agcaggagaa gtggatcctg caggcggagc tgttcaacga gatccatcgt tgggccacgt
    11341 cggagtggaa gcagatcaag aagagctcga gctggcagcg cgccgccgag cgctactacg
    11401 agatggtgcg cgacttcctc cgcgccgccg agaaggcgtg gggcgaaggc gtgtggggcc
    11461 acccgagcta catggtcacg cggcccgtca cgatcaaggc gatgctccgc gtgtgcgcgg
    11521 acctcgccgc ggccgatccg gatccggacg atcgcgtgaa gcggtgggcg cgcaagctgt
    11581 cgccgtgggg cgacttcgcg aagagcttcc gccaggaggg cttctacgag cgcttcccag
    11641 cgaagggcca gatcgagcgc gtcgcgcgca tccacaagga gctcgcgcgc gccgcgaaca
    11701 tcgagcgccg tcagaaggac aagggcgagg acgaggaata gtcctcgagc gcggcgaaca
    11761 tcgagcgccc ttagaacgac aagggcgcgc aggtcattga cggactgcga tctgcaggcg
    11821 gtcttggcca gggctcacgc gccgcgggat atgcttcgac cgctgcatgg cgggcgagca
    11881 catctacttc acgtggcctg atcgccggct gcgctatgac tgccgcggct gcggcgcatg
    11941 ctgcaaaggg ctcggcgtcg gcatcgatgt cgtcgctggc cagctcgtcc agctcgtgac
    12001 gaggcggccc gagctcgccg cgttcgtgcg tcggcgcggc gacgcggtca ccgcgttcaa
    12061 cccgcgcgat cgctgctggt tcctggccga cgacggcttg tgtcgcgtcg aggtcgagga
    12121 tggtcgcgcc gcgaagcctg cctcgtgccg gttgttcccg ttcaaccgcg tgttccgcgc
    12181 gggcgcgtac acgatcgtcg actacaacag cgtgatctgc ccactcgcgg tggtcgctag
    12241 cgacggcgtc tcgcacgccg aggtgctcgc cgagatcgcg acgatccagg atccggcgat
    12301 cgtcggcacg cagctgccac ccgacgatgc gctgttcgcg cgcgagcaag cgtacgccgc
    12361 cgacctgttg gcgcgcggtg ccgacgccat cgttgccgat gccgtggtga acgccgcatt
    12421 cgacaccatc ctcggcacac cgtggcgcgc accggctgcg ccgacactcg ccaacgcgct
    12481 gatgctgacg ccgtcgctgc gtttcaacga gctttacggc ccgcgccagt acgcgccgcg
    12541 cgaccgcatg cagtcgctgc tcacccgcat gcagctggcg tggctcggct tcgccgcgct
    12601 cggcgaacag ctcgcgcagc gcacgctggg gttgcaggag ctgacgacgc tatggtccga
    12661 gcaagcaccg ctcatgcatg cgatcgcgcg ctgggatgcc gtgccggtgt tcaaacctgg
    12721 acccgtcgag ctgccgagtg tcgatccgca caacctggtg cgcaccctcg gccagcgctg
    12781 cgtcgacaat cgcaacgcaa agcacacgct cggcgcgttg gtcgctgatg tgctcaccgt
    12841 cgcagatccg gccacacgcg tcgcggcgct caaaatggca gatccgatcc tgcgcgcggt
    12901 agcatggagc tagtgcgcgg ctcggttgcc atgctcgttg tgtcaggcgc gtgcgcgctc
    12961 gtcggcggct gcgacgtcgt gtttcgtctc gatcacgtcg gctcgcacgg cgatggcggc
    13021 gtcaccggcg acgtcgatga cgccatgacc gtcgatgcac ccccgagcaa cacgtgcatg
    13081 ttgagcccgt ccgtcttcgt tcccgagatc accgccaact atgaagatcc tgcgcagagc
    13141 ggagcgaagc tcgagctgtt cctgtcgttg tacgacaccg gcgtcgccct cgcggacatc
    13201 tacgtcgcgc gacgcgaagc tgtgagtgac ccgtggggcg ttccgctggt cgaaaacggg
    13261 ctgtccagca cggccgacga caccgatccg gtgctcgcac agaacgacac gctcgcgttg
    13321 tggacgtcga cccgcgttgc aagcactcgc aagatcttcc aggccatgcg cgacggcccg
    13381 aaccggccgt tcaacacgcc gatgatcgtg ttcgactcca ccgtcagtgg catcgacgtc
    13441 tcgcccgatg gcctgacgct gtacgtctgc gacggcaacg gcgtgatggg ccagatctct
    13501 cgcgctgcta ccacggtccc gtggttaccg acggcggcga cggtggtcgg caacggcatc
    13561 tactttccgt cggtcgccgg cgacgggctg acggtctact accatcgcaa caccacgctc
    13621 tatcgtcaga cgcgaatggc cccgagcggc ccattctcga acgaagtggc aatcgcggcc
    13681 ggcaccgacg cgaacatctc cgacgacggc cgcgagctgc tctacttcac tgatacgtcg
    13741 ctcgcgcgcg cgacctgcca gtagatagag gcgtcactac cagacgacgc acgcgttcgc
    13801 cgggcgcatc ttcgcgccgg ccttgcagcg gaacgccttc gcgaagtcgg gcgtgtccga
    13861 cagggtgccg ttgacgcgcc acctgccgga cgagtgcacg tcgacggtgg cgagcatctt
    13921 ttcgaactcg gggcgcatct tctcgcacca gacctggccg aacgcgagga agaactgctg
    13981 gtcctcggtg aagccgtcgg caacaaccgt ctccggcgcg cttgcacgca gcgcgcgata
    14041 gccggcgagc gacagcttga cgccgccgat gtcggcgatg ttctcgccga cggtgttcgc
    14101 gccgttgagc ccgtacttgc tgtactgatc gatcacgcac tgcgtgcgct gcttgaactg
    14161 cttctcggtc tccggctgcc accagttctt catgttgccc tgcgcgtcgt actgcgcgcc
    14221 ttggtcgtcg aagccgtgtg tcagctcgtg gccgacgacg gtgcccatgc cgccgaggtt
    14281 gacggggatc gagaactcga cgctgtagaa cggcggctgc aggatgcccg cggggaacac
    14341 catcttgttc tggttcgggc tgtagaacgc gttgaccgtc ggcgccgaga agccccaatc
    14401 gtcgcgatcg accggcttgc cgatcttcgc gatctggcgc gcgttctctg ccttacgacc
    14461 ggcgagcgca ttcgcggccc aggtctttgc atcgatcttg aacttgtact cgtgccactt
    14521 cgccggatag ccgatgtagt tggccatcgc gccgagcttc tctcgcgcgc gcgtcttcgt
    14581 cgtcgcgtcc atccacggca gcacgtcgag gttcgcctgc atcgcagacg tgatcgcgcg
    14641 cacctgttcc tccgcagcgc tcttgctcgc acccggaaac cgatcgcgca cgaacacctg
    14701 gccgagcaga tcgccgagcg cggcgtcggt gcggccgatg cagcgcttcc agcgcggctc
    14761 ttgttcgggc tggccggtca acgactgcgt gtacttgaag cgcgcctcct cgaggtcctt
    14821 cgtgaggtac gacgcggtcg agagcagcgt gcgcgcgacc aggtaattgc gccacacggc
//
LOCUS       DASUFX010000002         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_229,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000002 DASUFX010000000
VERSION     DASUFX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VFV99_00065"
     CDS             complement(<1..1620)
                     /locus_tag="VFV99_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057711.1"
                     /translation="MRDGLVGVKRMEKAFQRQVIYGGSLDTILLEMNLVPEERLTQYL
                     ALASGLPPAARDEGGAIEPSALALITAEIAAQFRAVPLSIDGDALRVLVCSPLEISEL
                     EDLADLLDRPLQPLITPEYRWHLVYSTAYRVEPPARFTTLARQLEVDATPVPVGRAKS
                     VIIDEQPIHDSQATAPLVAPSLTPQPPVLPPPSDGRVAAMPEPSDDATERVDMFEAHP
                     EVRNRTTTMLGLQPNRAQTASNPPPTISRTSTDEGLGGHRRPPGPATPVHGVPVQTPT
                     SVNTPPGGMQLRPPASVSTPPSGVRNVAPPMGVPALPSLTPARGVPVRPSTPPPVPKS
                     KGVPTAVVDSGSKVTSRGAAVRARASSEPIATAGRDSPLAAVRARELLATAEDRDTVF
                     LALLRAARARARYAGLLTVQGGAAIGRLALAEVGIDVSAVQKVLIPLDLVSPFRAVVQ
                     NQQPHIGPLQSGDPSIDSMVLRLGGSMPPSALLMPIVLRDRVVAVVIAHRVHSDLKLV
                     DVTELLPLASAAADALGRLIVRHKSAGYRAPS"
     gene            complement(1649..2266)
                     /locus_tag="VFV99_00070"
     CDS             complement(1649..2266)
                     /locus_tag="VFV99_00070"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5057712.1"
                     /translation="MSEREPAVLRDEPAGGTGGASSAAAPATGTPAPAKQPSPALQRP
                     TNKREAASVYVVGFWKRFAAAAIDFFVILPVAMLITFIASKVANVHLPAKNIKLFDID
                     MWIDLVLAGSSALVMALVLFIAISLTYLLVFHITLGRTLGMRVLKMKIIDVYGDPPSP
                     ARCVARCAGYLAGVATLFLGFLWIGFDNEKRGLQDWIAGTYVIRV"
     gene            complement(2310..3548)
                     /locus_tag="VFV99_00075"
     CDS             complement(2310..3548)
                     /locus_tag="VFV99_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX5057713.1"
                     /translation="MTALRQLSEQLLSGAVSIDQAQPMAPRFLAEPVAAGSYFVSSFA
                     NVSAFDTDDGLVLVDTGSFVLAEPTRARLRGITKRPVHTAIWTHGHVDHCFGVDLYER
                     DSGRHVRVIAHEAVTRRFERYKLTRAWNTAINQRQFQTNTEFPGEFRVPDETYQRALT
                     TVVGGRRFELHHALGETDDHTWVWVPDAGVICTGDLFIWASPNCGNPQKVQRFPREWA
                     QALRAMAALEPDVLLPGHGVPIWGREAVRRALDETATLLESLVDQVVTLMNAGARLDE
                     IMARVSVDPALLARPYLNPIYDEPEFIVRNLYRLYGGWWDGNPAHLKPPRDGALAKEI
                     VALGGGPSAVATRANRLATDGELALACELIEMAALAAPDNTTIQGIRADIYSQRAKQE
                     RSLMARGVYTAAADSSRRKS"
     gene            complement(3872..>4184)
                     /locus_tag="VFV99_00080"
     CDS             complement(3872..>4184)
                     /locus_tag="VFV99_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057714.1"
                     /translation="LWHRGGTNESRGTRLAISPQYCQPWLRPQESQLLIVPPDAARSC
                     SERMRSMLGYSIHPPFIGQVDGMHPLRLVDAGYAQHKTEARVVADRVLERPTSSMVDP
                     S"
BASE COUNT          688 a         1408 c         1436 g          652 t
ORIGIN      
        1 gctcggcgcg cgatagccgg ccgacttgtg gcgcacgatc agacggccga gcgcgtcggc
       61 agcggcgctc gcaagcggca acagctcggt gacatcgacg agcttgaggt cggagtgcac
      121 gcgatgcgcg atcacgacgg cgaccacgcg atcgcgcagc acgatcggca tcaagagcgc
      181 actcggcggc atcgagccac cgagtcgcaa caccatcgag tcgatgctcg gatcgccgct
      241 ttgcagcggc ccgatgtgcg gctgctggtt ctggacaacc gcgcggaacg gcgacaccag
      301 atcgagcggg atgagcacct tctgcaccgc gctgacatcg atgccgacct cggcaagcgc
      361 gagtcggccg atcgctgcgc cgccttgcac ggtcaacaaa ccggcgtagc gtgctcgtgc
      421 acgcgccgca cgcagcagcg cgaggaacac ggtgtcgcga tcctcggcgg tggcgaggag
      481 ctctcgcgcg cgcacggctg cgagtggact gtcgcggccc gcggtcgcga tcggttcgct
      541 cgatgcacgc gcccgcaccg ctgcaccacg cgaggtcacc ttcgacccgc tatcgacgac
      601 cgctgtcggc acgcccttcg atttcggtac gggcggcggc gtcgacgggc gtaccggcac
      661 accacgggct ggcgtcagcg acggcagcgc tggcacgccc atcggtggcg cgacgttgcg
      721 gacacccgac ggtggcgtcg agaccgacgc aggtggccgc agctgcatcc caccgggcgg
      781 cgtgttcacg ctcgtcggcg tctgtacggg aacaccatgc accggcgtcg cgggtccagg
      841 cggacgtctg tggccaccga gcccttcgtc ggtgctcgtg cgcgagatcg tcggcggcgg
      901 attgctggcg gtctgcgcgc gattcggctg cagcccgagc atcgtcgtcg tgcggttgcg
      961 cacctccgga tgcgcttcga acatgtcgac gcgctcggtc gcatcgtcgg acggctccgg
     1021 cattgcagcc acacggccat ccgacggagg cggcaagacc ggcggctgcg gcgtaagcga
     1081 cggcgcgacg agcggtgcgg tggcctgcga atcgtgaatc ggttgctcgt cgatgatcac
     1141 gctctttgcg cgcccgaccg gcaccggtgt cgcgtcgacc tcgagctggc gtgcgagcgt
     1201 cgtgaagcgt gcgggcggct cgacgcgata ggcggtcgag tagacgaggt gccagcgata
     1261 ctccggcgtg atcagcggct gcaacgggcg atcgagcagg tcggcgagat cttcgagctc
     1321 gctgatttcg agcggcgagc acacgagcac gcgcagcgca tcgccgtcga tcgacagcgg
     1381 cacggcgcgg aactgtgccg cgatctcggc ggtgattagc gcgagcgcgc tcggttcgat
     1441 cgcaccacct tcgtcacgtg ccgctggcgg caaacccgac gcgagcgcga gatactgcgt
     1501 cagccgctcc tcgggcacga ggttcatctc gagcagaatc gtgtcgaggc tgccgccgta
     1561 gatgacctgg cgctggaacg ccttctccat gcgctttacg cccacgaggc cgtcgcgcac
     1621 caagagggag ctcagacgcg atggcatctc agaccctgat cacgtaggtc ccggcaatcc
     1681 aatcctgtag gccgcgcttt tcgttatcaa agccgatcca cagaaagccg aggaacagcg
     1741 ttgcgacacc agcgaggtaa ccggcacagc gcgccacgca tcgcgcaggg gacgggggat
     1801 cgccgtagac gtcgatgatt ttcatcttga gcacgcgcat gccgagcgtg cggccgagcg
     1861 tgatgtgaaa gacgagcaag taggtcagcg agatcgcgat gaacagcacg agcgccatca
     1921 ccagtgccga gctccctgcg agcacgagat cgatccacat gtcgatgtcg aacagcttga
     1981 tgttcttcgc cggcaggtga acgttggcga ccttgctggc gatgaacgtg atcagcatcg
     2041 cgaccggaag gatcacgaag aagtcgatcg ccgccgcggc gaagcgcttc cagaacccga
     2101 ccacgtagac cgatgcggcc tcgcgcttgt tcgtcggtcg ctggagcgcg ggcgaaggct
     2161 gcttggccgg cgccggtgtt ccggtcgcgg gcgcagcagc gctgctcgcc cctccagtcc
     2221 cccctgcggg ctcgtcgcgt aatacggccg gctcgcgctc cgacatcaac gatttatacg
     2281 gcgccgccgc ccgtttgcgc aaatcctgtt caggacttac gacgggagct gtcggcagcc
     2341 gccgtgtaca cgccccgggc catcagcgac cgctcctgct tggcccgctg cgagtagata
     2401 tccgcgcgga tgccctgaat cgtcgtgttg tcgggcgcgg ccagggcggc catctcgatc
     2461 agctcgcacg ccagcgccag ctctccgtcg gtggcgagcc ggttggcgcg ggttgcgacg
     2521 gcgctgggcc cgcccccgag cgccacgatc tccttggcga gggcgccgtc gcgtggcggc
     2581 ttcaggtggg caggattacc gtcccaccag ccgccataga gccggtacag gttgcgcacg
     2641 ataaattcgg gctcgtcgta gatcgggttc aggtaggggc gggcgagcag cgcgggatcg
     2701 accgaaacgc gcgccatgat ctcgtcgagc cgcgctcccg cattcatcag cgtgacgacc
     2761 tgatcgacga gcgactcgag aagcgtcgcg gtctcgtcga gtgctcgccg cacggcttca
     2821 cgtccccaga tcggcacgcc gtgacccggc aacagcacgt cgggctcgag cgcagccatc
     2881 gcccgcaacg cctgcgccca ctcgcgcggg aaccgctgga ccttctgcgg attgccgcag
     2941 ttcggcgacg cccagatgaa cagatcgccg gtgcagatga cgccggcatc gggaacccag
     3001 acccaggtgt gatcgtcggt ctcgccgagc gcgtgatgca gctcgaagcg ccggccaccg
     3061 acgacggtgg tgagcgcgcg ctggtacgtc tcgtcgggga cgcggaactc gccggggaac
     3121 tcggtgttgg tctgaaactg gcgctggttg atcgcggtgt tccacgcgcg cgtcagcttg
     3181 tagcgctcga accggcgcgt caccgcctcg tgggcgatca cgcgcacgtg tcgtcccgag
     3241 tcgcgctcgt agagatcgac gccaaaacaa tgatcgacgt ggccgtgcgt ccagatcgcg
     3301 gtgtgcactg gtcgcttcgt gatgccgcgc aaccgcgcgc gcgtgggctc ggcaagcacg
     3361 aagcttccag tgtcgacgag gacgaggccg tcgtcggtgt cgaacgctga cacgttcgcg
     3421 aagctcgata cgaagtagct tcctgccgcg accggctcgg cgagaaacct cggcgccatc
     3481 ggctgcgcct gatcgatgct gacggccccg gatagcaact gttcggagag ctgtcgcaac
     3541 gccgtcatcg cgcacgcatg ctagcgcatc gccgcctccg atggcctcat gcgcttgccg
     3601 tgcgcgccac tcgtaaggcg cctttcgcgc cggcgtgctg cagagcgcga acacgcgagc
     3661 tgcacgccgc ggtggggcac tcgcgccgct gcatggaacg gagtgcacgc accacccacc
     3721 ggcgtggcgc tccatgtcgt tccggcgcac gactgggccg cctccccgct ggcgcttcgc
     3781 cgccccacct gcggcatgca tctcgcgttg acgagctgcg cgcgaccgac gcgcccgagt
     3841 gcacgccaac gtcgcgagcc gaacgaacga actacgacgg atcgaccatg ctggacgtcg
     3901 ggcgctcgag cacgcgatcg gcgacgacgc gagcttcggt cttgtgctgc gcgtagccgg
     3961 cgtccacgag gcgcagcgga tgcatgccat cgacctgacc gatgaacggc ggatgaatgc
     4021 tgtagccgag catcgaacgc atacgctcgc tgcacgaccg cgcggcgtcg ggcggcacga
     4081 tcaagagctg tgattcttgc ggtctgagcc aaggctggca gtactgcggg ctgatcgcga
     4141 ggcgcgtccc gcgcgactca ttggtgccgc cgcggtgcca cagc
//
LOCUS       DASUFX010000003         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_278075,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000003 DASUFX010000000
VERSION     DASUFX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278075"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFV99_00085"
     CDS             <1..547
                     /locus_tag="VFV99_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057715.1"
                     /translation="SEEKAEANFEKAQEHVKEESRLLQQAQKDLQAQQEDAIEKARRV
                     MRAEQELANARTRYKVEMTEQLARMDDRIAELQAKGDEKSSKTAERLQKHRDEFAQKL
                     QRLETEPPATWEHLDGTTNQPLRQRERMRQLKKGSAREPNQGSAEESTQGSAEQSTQG
                     SAEQPSQGSAEQPTEGSAKEP"
     gene            complement(602..>3480)
                     /locus_tag="VFV99_00090"
     CDS             complement(602..>3480)
                     /locus_tag="VFV99_00090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5057716.1"
                     /translation="TLTLGAGCSINDYCIGCELGGGDGGIGSGDGGGSGSSDGGMTCD
                     PMQVHPEMCNHADDDCDGNIDETFDLQTDLNNCGTCGNKCNKAGAQTTCGSGACAITG
                     CFPGFFDKNGDITGPYAMSDGCEYMCFQTNGGFEACDGVDNDCDGNTDETFDLTNDVD
                     NCGQCNKVCQFFQATPHCGNSVCSFDPTTDCAAGYIDIDGQQANGCEYQCTPTGAEVC
                     DLRDNNCNGSVDETFDLTSDVNNCGGCGFVCQFPHATPHCMLMTCGFNPATDCQAGFV
                     DVDGNQLNGCEYQCSKTNGGTEICDGIDNDCDGIADDSPVDAGAACSATSPPMGQCAA
                     NGTLSCSNGSLVCINATQPVVETCNNLDDDCNNTIDNGVTQSCYTGPMNTSGVGLCHN
                     GTATCAAGVFGACMGQVVPVAELCNNLDDNCNGMIDDGPGGQAITQSCYTGPMGTSGV
                     GLCHDGTRTCAFGAFGACVGQVTPITDICGDGLDSDCDGQNDAQEGCIVVNAEQRLDG
                     GGNAGALGTAAAARHDYDLAFARGGNPLGSRVYAAWSHLEGTVTEVYFRRSLDGGSTW
                     DPIINVTSGVSATAVKPIIAVSPGASDRVVIAYQTVNSGVRDIVVSVSADSGGTFGAA
                     SAALDAAGDSFHQSVAVRGTTVVVAWEKLDTTTLDRDIQSRASTDGGATWGTERKINV
                     GSPANGRFAGRPQVGLTSAGAAIWAWREKRSNTTLDIFAAVSANPNNAPAADIRVDTD
                     PASNDDDFPQLVVEETSAYLVWQEVSGVTNGGSDVMFARSTNGGTSWSAERIIDDPVT
                     EVSSSFTPVLAVDPKAAGGADDLVAIAWEDRRQGTQVYTSTSTNGGAAFGTPVRASNN
                     AGAPISGTTSAPQIASAGSGVLVVVYQNKQGQNPSHVFTASSIDSGATWSYTHVPLDT
                     GAGSAITPQVVGSIVANKPAAVAGWTDFRTNGVNGDIYTALAHR"
BASE COUNT          604 a         1108 c         1162 g          606 t
ORIGIN      
        1 tagtgaggag aaggccgaag caaacttcga gaaggcgcag gagcacgtca aggaagagtc
       61 gcggctgttg caacaagctc agaaggacct gcaggcgcaa caggaggacg cgatcgagaa
      121 agcgcgcaga gtcatgaggg cggagcagga gctcgccaat gcgcgtaccc ggtacaaggt
      181 cgagatgacg gaacagctcg cgcgaatgga cgatcggatc gccgagctcc aggcgaaggg
      241 cgatgaaaaa tccagcaaga cagcagagcg gttgcagaag caccgcgacg aattcgccca
      301 gaagctgcag cggctcgaga ccgaaccgcc agcgacctgg gaacacctcg acggcacgac
      361 caaccagccg ctgcggcaga gagagcggat gcggcagctg aagaaaggca gcgctcggga
      421 accgaaccag ggcagcgccg aggaatcaac ccagggcagc gccgagcaat caacccaggg
      481 cagcgccgag caaccgtccc agggcagcgc cgaacaaccg accgagggca gcgccaagga
      541 gccgtaacga aagcccgtcg cacgagagca gcgctcggcc aggcgttgcg ctggctgcgc
      601 gttaccggtg cgcgagtgcg gtgtagatgt cgccgttcac gccgtttgtg cggaagtcgg
      661 tccaaccagc aacggcggct ggcttgttcg cgacgatgct gccgaccacc tgcggggtga
      721 tcgccgagcc ggcaccggtg tcgagaggca cgtgcgtgta cgaccaggtc gcgccggaat
      781 cgatcgacga cgccgtgaag acgtgcgacg ggttctggcc ctgcttgttt tgatagacga
      841 cgacaagcac gccgctgcct gcgctcgcga tctgtggcgc ggacgtcgtg ccggagatcg
      901 gcgcgccagc attgttgctt gcgcgtaccg gcgtgccaaa cgccgcgcca ccattcgtcg
      961 acgtcgacgt gtagacctgc gtgccctggc gccgatcctc ccatgcgatc gcgacgagat
     1021 cgtcagcgcc gccggccgcc ttcggatcga cggcgagcac tggcgtgaac gacgacgaaa
     1081 cctcggtgac tggatcgtcg atgatgcgtt ccgcactcca cgacgtgccg ccgttcgtgc
     1141 tgcgcgcgaa catgacatcg gagccgccgt tcgtcacacc cgagacttct tgccagacca
     1201 gatacgcgct ggtttcctcg acgacgagtt gcgggaaatc gtcgtcgttg cttgccgggt
     1261 cggtgtcgac tcggatgtcg gcggcgggcg cgttgttcgg gttcgcagac actgcggcga
     1321 agatgtcgag cgtcgtgttg ctgcgcttct cgcgccacgc ccagatcgct gcacccgcgg
     1381 aggtgaggcc gacttgcggg cgaccggcga agcggccgtt ggcagggctg ccgacgttga
     1441 tcttgcgttc ggtgccccac gtcgcaccgc cgtcggtgga cgcgcgactc tgaatgtcgc
     1501 gatcgagcgt cgtcgtatcg agcttctccc acgcgacgac gaccgtcgtg ccacgaacag
     1561 cgacggactg gtggaacgag tcaccggcag cgtcgagcgc ggcgctcgcg gcgccgaacg
     1621 ttccgccgct gtcggcggag accgagacaa cgatgtcgcg cacgccgctg ttcacggtct
     1681 ggtacgcgat gacaacgcga tcgctggcgc cgggagacac cgcgatgatc ggtttgactg
     1741 ccgtcgccga gacacctgac gtcacgttga tgatcggatc ccacgtcgag ccgccgtcga
     1801 gcgaacgccg gaagtacacc tcggtcacgg tgccctcgag gtggctccac gccgcgtaga
     1861 cacggctgcc gagcggattt ccgcctcgcg cgaacgcgag atcgtaatcg tggcgcgcgg
     1921 ccgccgcggt gccgagtgcg cctgcgttgc cgccgccgtc gagccgttgc tcggcattga
     1981 cgacgatgca gccttcttgt gcatcgttct ggccatcgca gtcggaatcg agaccatcgc
     2041 cgcagatgtc agtgatcggt gtgacctgac cgacgcaggc gccgaacgcg ccgaacgcgc
     2101 atgtccgggt gccgtcgtgg cagaggccga cgccgctcgt gcccatcggc ccggtgtagc
     2161 agctctgcgt gatcgcctga ccgccggggc cgtcgtcgat catgccgttg cagttgtcgt
     2221 cgaggttatt gcagagctcg gcgaccggca cgacttggcc catgcacgcg ccaaacacac
     2281 cggccgcgca cgtcgcggtg ccgttgtggc acaggccgac gccgctcgtg ttcatcgggc
     2341 ccgtgtagca gctctgcgtg acgccgttgt cgatggtgtt gttgcaatcg tcgtcgaggt
     2401 tgttgcaggt ctcgacaacc ggctgcgttg cgttgatgca cacgagcgag ccgttcgagc
     2461 acgacagcgt gccgtttgcg gcgcactgac ccatcggcgg cgatgtcgcg gagcacgcgg
     2521 cgcccgcatc gacgggactg tcgtcggcga tgccgtcgca gtcgttatcg atgccgtcgc
     2581 agatctcggt gccgccattg gtcttgctgc actgatactc gcagccattc agctgattac
     2641 cgtcgacgtc gacgaagccg gcctggcaat cggtcgctgg attgaatccg cacgtcatca
     2701 gcatgcaatg cggcgttgcg tgcggaaact gacagacgaa gccgcaacca ccgcagttgt
     2761 tgacgtcgct cgtgaggtcg aacgtttcgt cgacgctgcc gttgcagttg ttgtcgcgca
     2821 ggtcgcagac ctcggcgccc gtcggcgtgc attgatactc gcagccgttc gcctgttggc
     2881 catcgatgtc gatgtaaccg gccgcgcagt ccgtcgtcgg atcgaagctg cacacgctat
     2941 ttccgcagtg cggcgttgcc tggaagaact gacagacctt gttgcactgg ccgcagttgt
     3001 cgacgtcgtt tgtcaggtcg aacgtttcgt cggtgttgcc atcgcaatcg ttgtcgacgc
     3061 catcgcatgc ctcgaatccg ccgttcgtct gaaagcacat gtactcgcaa ccgtcggaca
     3121 tcgcgtacgg cccggtgatg tcgccgttct tgtcgaagaa tcctggaaag cagccggtga
     3181 tcgcgcacgc gccgcttccg caggtggtct gcgcgccggc cttgttgcac ttgttgccgc
     3241 acgttccgca gttgttgaga tcggtctgca gatcgaacgt ctcgtcgatg ttgccgtcgc
     3301 agtcgtcgtc ggcgtggttg cacatctcgg gatggacctg catcgggtcg cacgtcatgc
     3361 cgccgtcgct gctgccgctg ccaccgccgt cgccggagcc gatgccgcca tcgccgccac
     3421 cgagctcgca gccgatgcag tagtcattga tcgagcaacc tgcgcccagc gtgagcgtcg
//
LOCUS       DASUFX010000004        16426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_278268,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000004 DASUFX010000000
VERSION     DASUFX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16426
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278268"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VFV99_00095"
     CDS             complement(<1..1443)
                     /locus_tag="VFV99_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5057717.1"
                     /translation="MVAGKSEAMARQLEAAQAITHVGSWEWDIASGAVTWSDELYRIY
                     GIEPGSKPITLEVFLGSLHPDDVSRIREEINKALQQQGGARFSYRERIVRPDKSVRVL
                     DTVGEIVCGADGQATSLLGTCRDVTEEIQHAEERRRDARVQSGERQALELLAAGAPLA
                     EVLETIVLYIEDVSPGTIASILLLDGSGEHLRHGAAPHLPADYNRAIDGAAIGPRAGS
                     CGTAAYRKEPVFVTDIMTDGLWIAYRHLVEPYGLRACWSLPICNSDGVVLGTFAVYHR
                     EPRSPDAASVELLERAAHVANIAIERRHLDEQLRALSARIEAVREDERTNIAREIHDE
                     LGQALTALKLDIGWVARRAPEGELAAKLGEMSRETDDIINAVRRISAELRPGILDSIG
                     LRAALEWQADEIERRTGIKCNVVSSVGDLQLERSLATTVFRIFQEATTNVVRHAKATR
                     IDVSLWLERGNLRLDIADDGVGVPEISPRNS"
     gene            1475..2101
                     /locus_tag="VFV99_00100"
     CDS             1475..2101
                     /locus_tag="VFV99_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5057718.1"
                     /translation="MQRVLLVDDHAVVRRGVRAILEDHLPAVAVSEASNGDEALAAIA
                     EKFDAVVLDLSMPGRDGFDLLTEIKHRQPKLPVLIMSLHGEEQFALRALRAGAAGYLT
                     KSTAPEQLVAAITKVLRGGRYISESLAERLAADVGLEAPGTPHERLSDREFDVMRGIA
                     SGRSVSEIAAQMHLSVKTVSTYRTRLLEKMGMSSNAELTRYALEAGLV"
     gene            2195..3247
                     /locus_tag="VFV99_00105"
     CDS             2195..3247
                     /locus_tag="VFV99_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5057719.1"
                     /translation="MKILIVDGSREQRLHLVQVLGAVTNIVIQGAVSDMRSALHAVVE
                     ASPDVIVTGAALPDGDGAELIASVRKLARSPSFVVVAGEPSDELRARYLAAGVDRYLE
                     APQDARALQVAVSTLRRRATGSIPPEETQRLLGRMTSGVVHDLNNYLHVLDVTFTLLR
                     RHPEDPQLWKQIQSAILAMGRLNATLLAYARGSALAPALVDLAALARDTVAVLGRVIP
                     ADIVVRFDIDDQLPPVQGVRSELEQLVLNLVMNATDAMPKGGELVISLKRSAGAVVVL
                     EITDTGAGVAVLPTNGRSQSTKRDGVGLGLGIAQAVVDRHRGALNVSPLERGGTKAIV
                     MFPTTRSLTKLELRDE"
     gene            complement(3250..4671)
                     /locus_tag="VFV99_00110"
     CDS             complement(3250..4671)
                     /locus_tag="VFV99_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5057720.1"
                     /translation="MAHPEVVRVFATSQDPDFDGPWQARTPSNSTGSAVVIGPGLLLT
                     GAHVVANATFLQIQKLSHPDKAIARVKAVSHDCDLALLEVVEPVDFLSDIEPAEVGDM
                     PRLRDEVAVVGYPVGGEEISITEGVVSRIEVQRYSHSQRHLLAVTVDAAINAGNSGGP
                     VFGDGKVVGIAFQKLTGVDNIGEMVPPPIIRAFLQGVEKNKRPEIPALGITTQNLENP
                     LLRKQLGLGDGERGVVVLHVDHGGSADGVLQPRDVITTIDGLPIANNGTVQYMNRYRT
                     RYDVVLGYRYIGDKVKLDILRGGKQMKVELQLAPWQPLVPRSRYDQPPQYFVYGGLVF
                     QTLTRDYLTTWAKWWNKAPKEFLNYYYLGYRSPEQHEVVILTQILADEINVGYAHLYN
                     EAVATLNGKAPLDMEDFVTRLSAARGVVEITTTSGGMIMLDADEVRKATARILTRYHI
                     PRDRTPGLPGAQPATPPLRAALP"
     gene            4775..5800
                     /locus_tag="VFV99_00115"
     CDS             4775..5800
                     /locus_tag="VFV99_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5057721.1"
                     /translation="MASAVPAVSLAEARKLIAQVHRGEPIAASSAIRRTSAEAVRAIG
                     SVPSLDVITETPSQLDPFVKYLLAAPDGARFETVRIPLEHPDRFCVCVSSQVGCALGC
                     TFCATGRLGLTRNLHAWEIIEQIRVVRARLPVDPVRRSESGALRFRRVHGVVFQGMGE
                     PLANLDEVLDAIAVLTEPSALGIDARAITVCTAGLPVGIRRLASDAPKVRLGLSIHTA
                     LPERRKSLMPIARAHDLDDVLAAAAEHARITGLAPMWAITLLDGINDSDDDARALAAR
                     ARAFAETTGVLPRLSIIAYNAIGPDDPYRRSTRETAYRDALGLPNHRRYSGGSDVGAA
                     CGQLALR"
     gene            5813..6346
                     /locus_tag="VFV99_00120"
     CDS             5813..6346
                     /locus_tag="VFV99_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5057722.1"
                     /translation="MAHPVHDIEVPADIGTFVPTVIEIPRGSHLKYEVDKPTGLLRLD
                     RVLYSAVFYPANYGFIPRTHADDGDPLDILVLMQEPVHPLTIVNARAIGGLRMVDDKG
                     GDDKIIGVCIDDPAYEHYETLDELPPHIMKELERFFRDYKILEEKRVEVDTFYDRKRA
                     LEIIQTSRDAYDRGEGR"
     gene            complement(6343..6954)
                     /locus_tag="VFV99_00125"
     CDS             complement(6343..6954)
                     /locus_tag="VFV99_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5057723.1"
                     /translation="MLLYHDPRAPNPRRVRIFLSEKSVAYDTIEVLIANSAHQTDEFR
                     RKNPISLLPVLEFPDGRILRESMAISRYIEELHPEPNLFGADAWERAQVEMWNRHAEL
                     ELLWPISQVFRNTHPFWVGRIKQAPEFAGIMREHIGLRFDWLDGELGKRQYMAGDRFT
                     VADITALCAIDFGKVSDIRIKAETHPNLAAWHKRVSDRPSTKA"
     gene            7016..7945
                     /locus_tag="VFV99_00130"
     CDS             7016..7945
                     /locus_tag="VFV99_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057724.1"
                     /translation="MRIAGILVVLCVFLSLGAATGCKNKGDADAAPDPEAVKAQQELI
                     ARRDKLLDARKKLQSDKDKLDAEIKEIQAKGGDATEQMKKKAELENELESSNESIISL
                     VNSKLDGLGKAADKTAQVASREAEIASREKAVAEREAKLADRERQLAQRDSESAARWK
                     ETCSVGAPMIIQATAPKDGKYTNKDVTALIAKAKKEMNKKNLINSDLGAAASLESEAG
                     KALNENDMSKAYFAAAQLVATIDAITINRQFIQAKTARLSAQAKGTKLDEATNKQIAD
                     GLGDVMQKYNDGDFSAANRRLNQLASILAKSGG"
     gene            8418..9116
                     /locus_tag="VFV99_00135"
     CDS             8418..9116
                     /locus_tag="VFV99_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057725.1"
                     /translation="MRRAGLLLLALTGCANIFGLDSPQMRDDGGAGSDTGSDGPPVSD
                     CHSDNFDDNALNPGLWAKFVEKMETVTETNQQLAITLDNTTGSAYSGIDGVLPLAADE
                     TGVQVEVVQWSGNDQSETALVLVLNGPNQLIIGKDGNSLRQTVKTGGADMTHMSPFDA
                     SIRFLRIERRLSDTKVTFLSSTDGTAWNLLFMTNASFADQALKPQLYAGHYQQVPATT
                     VIFDNFVVLSPRCP"
     gene            complement(9120..9989)
                     /locus_tag="VFV99_00140"
     CDS             complement(9120..9989)
                     /locus_tag="VFV99_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5057726.1"
                     /translation="MLSRAVFLLTLLATTSVYADRVVAITPLSTLGAEDKSVATKKLV
                     TQIEQAFAALPGTKVVPAAQVSAAIDKAKKPALKQCEGEAGCVTEVGKLVSAQFVVTG
                     EVGGLGDSKVVYLKLIDVGAARELRSTTLAVGVSDSPMGAAVRLSDPDKYRGNVRFGF
                     DVGGATVLVNGTKVQLSATKDIALPVGTHAVTVTHPQYHNFVKFVEVTYGKTTDVQVT
                     MKQYPIVEHDIQEKPGSRDTIIYTDPPLWRRPYVTGPVIAILAIGAGFLAAKIAHDFP
                     DYDNCRKVGGMGC"
     gene            complement(9983..10864)
                     /locus_tag="VFV99_00145"
     CDS             complement(9983..10864)
                     /locus_tag="VFV99_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5057727.1"
                     /translation="MARVASLVIGVVVLAVALRAQAQPAPDELLDKKIAVWRFDALGI
                     DNEIVQRLETLFRMELDRLDKVPQPSRRDIERSVTSAEQNCTGEEKCLAAIGKRLGVE
                     WVVTGTVGSLGDNYVLNIKVVDVATGKSQRIQSDPLRGSPDELIEGVRVAAYRLLAPQ
                     QIHGSLQIQSDLIGAEVTLDGNKLGKTPLANGGVIAKLPLGKHRLRVEAKSYDPFDSD
                     VEVHFQKVSPVVVRLLQASGPVGTGKTIYKERSPIYTRPWFIGAVGVGVVALAVLIGA
                     SAGHVDCYINGDPSTPC"
     gene            10919..11344
                     /locus_tag="VFV99_00150"
     CDS             10919..11344
                     /locus_tag="VFV99_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057728.1"
                     /translation="MSWLRGVAIAAFVTSAVACSSISTVRVQSENVHVAKGLRPIAVV
                     HAQVTSFELLFFEIPGHVTLDYVVNRMLIHAAKQMGADKVVDIKVDITPDDGIWVLRR
                     IFGWRSAQASGVAVVVEDEAQSAPPAATPPGPPAPTPSK"
     gene            11359..12282
                     /locus_tag="VFV99_00155"
     CDS             11359..12282
                     /locus_tag="VFV99_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX5057729.1"
                     /translation="MPLEVFDRRLILVVGKGGVGRSTVAAAIAGKCAAAGRRTLLFET
                     NANDRFGSYFDKPPVGTELTQLAPNLWAVNTNPAAALEEYGLMILKFKSVYEMVFENR
                     VTKAFLRAIPGLDDYSLLGKAWFHTTEEKRGKPIWDNIVFDMPASGHSVSMLRIPWVI
                     TDTVPEGPLTRDARTVKELLCDRARTATVLVTLAEEMPVNEAIELEAKLTELGIVPQQ
                     IVCNQVYPNHFPPGAPVSRVLDALTADPTLQTPLREVVEHATLSRDRRALNERYLAEL
                     RRRARTPVHELPMLFAPTLEAAHVKALGDLL"
     gene            complement(12283..12480)
                     /locus_tag="VFV99_00160"
     CDS             complement(12283..12480)
                     /locus_tag="VFV99_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057730.1"
                     /translation="MISYPYIGRGMVIFRDPHGHRMITTPVKRVLGEPGGKMIYVETE
                     NSVYRLEIHGEPLFPMRATGE"
     gene            12628..13521
                     /locus_tag="VFV99_00165"
     CDS             12628..13521
                     /locus_tag="VFV99_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5057731.1"
                     /translation="MSDPSVEALYEQVRTAVPTAPGEMPDALLPVAPGIRVLALRTPT
                     LPPAAHTNVYVVGPERGPQVAIDPGSPYADQQSLLAKAISQEADAGRPLACVLLTHHH
                     GDHVGGAVALATAFGVPIAAHANTARRLAGRVPVTRELVDREELYGVTCIYTPGHADG
                     HLCYEHGEASVVGDMVAGLGTILIDPIEGNMAHYLGSLAALLERPTRRLLPAHGPMIE
                     DGPSKLREYIAHRTMREDRVLAALKGKTDASVEDLVPIAYADTPKLLWPLAERSLRAH
                     LDKLVGEGRAREVGPERWTAG"
     gene            13544..14977
                     /locus_tag="VFV99_00170"
     CDS             13544..14977
                     /locus_tag="VFV99_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5057732.1"
                     /translation="MVRTFDTLPEVTLARDLLRRWYGLELGYAAGDGSGYERTSHAAC
                     ATLLAHPSGACGAQMKDVAAQLAAKKDGNAIVQRCYAGMTIVAAPVRDTNALAGIVYA
                     SGGRTGAVADVEAAIAKTTGATGEAATLAALAPELGDADIARVKDLVKSAAAAVERSQ
                     PATIPPTTWTHPFNEIVGDAPAVRDVIKLLNKVVKSEATVLVHGESGTGKELIARAIH
                     YHGPRAKKAFVVQNCSAFNDNLLESALFGHVRGAFTGAVKDQKGLFETADGGTFFLDE
                     IGDMSAALQVKLLRVLQEGTFMPVGGTKPVKVDVRIIAASHKNLATMVQQKQFREDLF
                     FRVNVLPITVPPLRDRVADIPRLAQHFVTKHWRNPGAAPKLSQSALARLVAYPWPGNI
                     RELENEIERMLVLAGDAQELVGSMVSSRVAGAGNAGRPLRDIVDVAEADAIVAALGRH
                     SGDRDAASAELNVSRAYLDARCTALGL"
     gene            14974..15180
                     /gene="yacG"
                     /locus_tag="VFV99_00175"
     CDS             14974..15180
                     /gene="yacG"
                     /locus_tag="VFV99_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX5057733.1"
                     /translation="MTKQTKGKCPSCRGEAIKEGNKLWPFCSERCHLIDLGRWLDEEY
                     RVPDEPDASGGGIESVTPHTKDDN"
     gene            15241..15615
                     /locus_tag="VFV99_00180"
     CDS             15241..15615
                     /locus_tag="VFV99_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057734.1"
                     /translation="MTGVPLGIMLGALNVVVIAIGMAGIGGESGIAMWVVMFGIVPGL
                     VIGALLGWLAEVMKPLPIWVRRFVLIVPAVLLVVVLAAEFSLEGFILLSCIPTAVATF
                     LLERGTRQVAPPPVPVAHARRS"
     gene            complement(15634..>16426)
                     /locus_tag="VFV99_00185"
     CDS             complement(15634..>16426)
                     /locus_tag="VFV99_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057735.1"
                     /translation="KEWASPSPARAKLVAQLETMEAATRSNKHSAFAGCDASTRAAWE
                     AQLKGLKLPKVPSEGAASAFVAATLGSPEGYLAYQALRLCSAGDENSSKGLDIVGADV
                     VQRGPRTATIAAWMASSAQIKFDDRGLKMNELLANGNLPMSEGARFQQQRQGVIAKIE
                     ENEGSVVISFKRVVEPREDCIKWKETDHIETFRADGSPLYRRECLKWGIVKMDLTAEP
                     LEVGKVMAQGLKPGMYLVGIEGFAIVATASAKSTKPIWVFGGSVK"
BASE COUNT         2769 a         5387 c         5456 g         2814 t
ORIGIN      
        1 cgagttgcgc ggtgagatct ccgggacgcc gacgccatcg tccgcgatgt cgaggcgtag
       61 gttgccgcgc tcgagccaca gcgagacgtc gatgcgtgtc gccttcgcgt gccgcacgac
      121 gttcgtcgtc gcttcctgga agatgcgaaa caccgtggtc gccaagctcc gctcgagctg
      181 gaggtcaccg acgctggata cgacgttgca cttgatcccg gtgcggcgct cgatctcgtc
      241 ggcttgccat tcgagtgcag cgcgcaggcc gatcgaatcg aggatgccgg ggcgcagctc
      301 cgccgagatc cggcgcacgg cattgatgat gtcgtccgtc tcgcgcgaca tctcgccgag
      361 cttcgccgcc agctcgccct cgggggcgcg gcgcgcgacc caaccgatgt cgagcttgag
      421 cgcggtgagc gcctgaccga gctcgtcgtg gatttcgcgc gcgatgttcg tgcgctcgtc
      481 ctcgcggacg gcttcgatgc gagccgacag tgcgcgtagc tgttcatcga ggtgacggcg
      541 ctcgatcgcg atgttcgcga cgtgggcagc gcgctcgagc agctcgaccg atgccgcatc
      601 gggcgagcgt ggctcgcggt gatagaccgc gaacgtgccg agtaccactc cgtctgaatt
      661 gcagatcgga agcgaccagc acgcgcgaag gccatatggc tcgacgaggt ggcgatacgc
      721 gatccacagg ccatcggtca tgatgtcggt gacgaacacc ggctccttgc gataggcggc
      781 cgtgccgcac gagcctgcgc gtgggccgat cgcagcgccg tcgatcgcgc ggttgtagtc
      841 ggcgggaagg tgcggcgcag cgccgtggcg cagatgttca ccgcttccgt cgagcaacag
      901 gatcgatgcg atcgtgccgg ggctgacgtc ctcgatgtac agcacgatcg tctcgaggac
      961 ctcggcgagc ggtgcacctg cggcgaggag ctcgagcgcc tgacgctcgc cggattgcac
     1021 gcgggcgtca cgcctgcgtt cttcagcgtg ctggatctcc tcggtgacat cgcggcaggt
     1081 accgagcagg ctcgtggctt ggccgtcggc tccgcatacg atctcaccga ccgtgtcgag
     1141 cacgcgcacg ctcttatcgg ggcgcacgat gcgttcgcga tagctgaacc gcgcaccacc
     1201 ttgttgctgg agcgccttgt tgatctcctc gcggatccgg ctcacgtcgt cgggatgcag
     1261 cgagccgaga aacacctcga gcgtgatcgg cttgctgccg ggctcgatac cgtagatccg
     1321 gtacagctcg tcggaccatg tgacggcacc gctcgcgatg tcccattccc aactgcctac
     1381 gtgcgtgatc gcctgagcag cctcgagctg ccgcgccatt gcctcggact tcccagccac
     1441 catggtcgtg gcgggtacgt tacaatgaag gaggatgcag agggtcttgc tcgtcgacga
     1501 tcatgcggtg gtgcgccgcg gcgtccgcgc gatcctggaa gatcacctgc cggcggtcgc
     1561 ggtctccgaa gcctccaatg gtgacgaagc gcttgcagcg attgcagaaa aatttgacgc
     1621 ggtggtccta gacctgtcga tgcccggccg cgacggcttc gacctgttga ccgaaatcaa
     1681 gcaccggcaa cccaagctgc cggtgctgat catgagcctg cacggcgagg aacagttcgc
     1741 gctgcgcgca ctgcgggcgg gcgccgcggg ctacctgacg aagtccacgg cacccgagca
     1801 gctcgttgcc gcgatcacga aggtgttgcg cggtggccgg tacatcagcg agtcgctcgc
     1861 cgaacggcta gctgctgacg tcggcctcga agcaccgggt acgccgcacg agagactctc
     1921 cgatcgcgag ttcgacgtga tgcgcggaat cgcgtccgga cgcagcgtga gcgagatcgc
     1981 ggcgcagatg catctcagcg tgaagaccgt gagcacgtac cgcacgcgac tgctcgaaaa
     2041 gatgggcatg tcgtccaacg cggagctcac gcggtacgcg ctcgaagccg gtctcgtgta
     2101 ggacggacgc cgacgcgcta agtcggcctg ctccgacatt cagatcgttg aagctccgac
     2161 ggtgtacgca cgtcatgcgc gctcgaatat ggtcatgaag attctgatcg tggacggctc
     2221 gcgagagcag cgtctgcatc tggtgcaggt gcttggtgcg gtgacgaaca tcgtgattca
     2281 gggagcggtg tccgatatgc ggagcgcgct tcacgcggtg gtcgaggcgt cgcccgacgt
     2341 gatcgtcacg ggcgcggcgt tgccagatgg ggatggtgct gagctgatcg cgagtgtgcg
     2401 caagctcgcg cgctcgccgt cgttcgtggt cgtggcgggc gagccctccg acgagctccg
     2461 agcgcggtat ctcgcagccg gcgtcgatcg ctacctcgaa gctccgcagg atgcgcgggc
     2521 gctgcaggtc gcagtcagca cgctgcgtcg tcgggccacc ggctcgattc cgcccgagga
     2581 aacccagcgc ttgctcggcc gcatgacgag cggcgtcgtt cacgatctga acaactacct
     2641 ccacgtgctc gacgtgacgt tcacgctgct ccgtcgtcat cccgaggacc cccagctgtg
     2701 gaaacagatc caatccgcaa ttctcgcgat gggcaggctc aacgcgacat tgctcgcgta
     2761 cgcgcgcggt agtgcgctcg cccccgcgct cgtcgacctt gccgcactcg cacgcgatac
     2821 cgtcgccgtg ctcggccgcg tgattcccgc cgacatcgtc gtgcgcttcg acatcgacga
     2881 ccagctacca ccggtgcaag gcgtgcgctc cgagctcgag cagctcgtgc tcaatctcgt
     2941 gatgaatgcg acggacgcga tgccgaaggg cggcgagctc gtgatctcgc tcaagcgcag
     3001 cgcgggtgct gtcgtcgtgc tcgagatcac cgataccggc gcaggcgttg ccgtgctgcc
     3061 gacgaacggt cgcagccagt cgacgaagcg cgatggcgtg gggctcgggc tcggtatcgc
     3121 gcaggcggta gtagaccgtc accgcggcgc gctgaacgtg tcgccgctcg aacgcggcgg
     3181 cacgaaggcc atcgtgatgt tcccgacgac gcgcagcctg acgaagctcg agctacgcga
     3241 cgagtaacgt cagggcaggg cagcgcgcag cggcggcgtc gcgggctgtg cacccggcaa
     3301 acccggcgtg cggtcgcgcg ggatgtgata gcgcgtgagg atgcgagccg tcgctttgcg
     3361 cacctcgtcg gcatcgagca tgatcatgcc gcccgacgtt gtcgtgatct cgacgacgcc
     3421 gcgcgccgcg gacagccgcg tcacgaagtc ctccatgtcg agcggcgcct tgccgttcag
     3481 cgtggcgacg gcctcgttgt agaggtgcgc gtagccgacg ttgatctcgt cggcgaggat
     3541 ctgcgtcagg atcacgacct cgtgctgctc gggcgagcga tagccgaggt agtagtagtt
     3601 gaggaattcc ttcggcgcct tgttccacca cttggcccac gtggtgaggt agtcgcgcgt
     3661 cagcgtctgg aacacgaggc cgccgtagac gaagtactgc ggcggctggt cgtagcgcga
     3721 gcgcggcacg agcggctgcc acggcgcgag ctggagctcg accttcatct gcttgccgcc
     3781 gcgcaggatg tcgagcttca ccttgtcgcc gatgtagcgg tagccgagca cgacgtcgta
     3841 gcgggtgcgg taccggttca tgtactggac ggtgccgttg ttcgcgatcg gcaggccgtc
     3901 gatcgtcgtg atcacgtcgc gcggctggag cacgccgtcg gcgctaccgc cgtgatcgac
     3961 gtgcagcacg accacgccac gctcaccgtc gccgaggccg agctgtttgc ggagcagcgg
     4021 gttctcgagg ttctgcgtcg tgatgccgag cgcgggaatc tccgggcgct tgttcttctc
     4081 gacgccctgc aagaacgcgc gaatgatcgg cggcggcacc atctcgccga tgttgtcgac
     4141 accggtgagc ttctgaaacg cgatgccgac gaccttgccg tcgccaaaca ccggaccacc
     4201 gctgttgccg gcgttgatcg cggcgtcgac cgtgaccgcg agcaggtggc gctgcgagtg
     4261 gctgtagcgc tgcacctcga tgcgcgacac gacgccttcg gtgatcgaga tttcctcacc
     4321 gccgaccggg tagccgacga ccgcgacctc gtcgcgcagg cgcggcatat cgccgacctc
     4381 ggccggctcg atatcgctaa gaaaatcaac cggttcgacg acctccagta acgcgaggtc
     4441 gcagtcgtgg ctgaccgcct tcacccgcgc gatcgccttg tcgggatgcg agagcttctg
     4501 gatctggagg aacgtggcgt tcgcgaccac gtgcgcgccc gtcagtaaca gtccggggcc
     4561 gatgacgacg gcggatccgg tgctgttcga cggtgtgcgc gcctgccaag gtccgtcgaa
     4621 gtcggggtcc tgtgatgttg caaagacgcg gactacctcg gggtgcgcca cagaaccagc
     4681 gtaccgtggt accgcgtaac cagcaaagtg taaggtctac atgatcctct aacgccacgt
     4741 aatgctcgca cttcaagcaa taacacctga acgtttggct tctgccgttc cagcggtatc
     4801 gctcgcggag gcgcgcaagc tgatcgcgca ggtgcatcgc ggtgagccca tcgctgcgag
     4861 ctccgcaatt cgccgcacat cggcagaagc ggtgcgcgcg atcggtagcg tgccctcact
     4921 cgacgtgatc accgagacgc cgagtcagct cgatccgttc gtgaagtact tgctcgcagc
     4981 gcctgatggc gcgcgtttcg aaacggtgcg cattccactc gagcatccgg atcgcttctg
     5041 cgtgtgcgtg agctcgcagg tcggttgcgc gctcggctgt acgttctgtg cgaccggccg
     5101 gctcggtctc acgcgcaatc ttcacgcgtg ggagatcatc gaacagattc gcgtcgtgcg
     5161 tgcgcgcttg ccagtcgacc ccgtgcgccg gagtgaatcc ggcgcactcc gctttcggcg
     5221 cgtgcacggc gtcgtgttcc agggcatggg cgagccgctc gcgaacctcg atgaggtgct
     5281 cgacgcgatc gcggtgctca ccgaaccgag tgcgctcggc atcgacgcgc gcgcgatcac
     5341 cgtgtgcacc gctggcttgc cggtcggcat tcgccggctc gcgagcgatg cgccgaaggt
     5401 gcggctcggg ttgtcgattc acaccgcact accagagcgt cgcaagtcgc tgatgccgat
     5461 cgcgcgcgcg catgacctcg acgacgtgct cgctgccgcc gccgagcacg cgcgcatcac
     5521 cgggctcgcg ccgatgtggg ccatcacgtt gctcgacggc atcaacgatt ccgacgacga
     5581 tgcgcgcgcg ctcgcagcgc gggcacgagc attcgccgaa acgacgggcg tgctgccgcg
     5641 gctatcgatc atcgcgtaca acgcgatcgg tcccgacgat ccgtatcggc gctcgacgcg
     5701 cgagaccgcg tatcgtgacg cgctcggtct gccgaatcac cgtcgctact cgggtggctc
     5761 ggatgtcggc gcggcgtgtg gccaactcgc gctgcggtga tacgttcccg gcatggcgca
     5821 cccggtacac gacatcgaag ttccggcgga cattggaacg ttcgtaccga cggtgatcga
     5881 aatcccgcgc ggctcgcatc tcaagtacga ggtcgacaag ccgaccggcc ttctgcgcct
     5941 cgatcgcgtg ctctacagcg cggtgttcta tccggcgaac tacggcttca tcccgcgcac
     6001 gcatgctgac gacggcgatc cgctcgacat cctcgtgctc atgcaggagc ccgttcatcc
     6061 gctcaccatc gtgaacgctc gcgcgatcgg tgggctgcgc atggtcgacg acaagggcgg
     6121 cgacgacaag atcatcggcg tgtgcatcga cgatccggcg tacgagcact atgagacgct
     6181 cgacgagctg ccgccgcaca tcatgaagga gctcgagcgc ttcttccgcg actacaagat
     6241 cctcgaggag aagcgcgtcg aggtcgacac gttctacgac cgcaagcgtg cgctcgagat
     6301 catccagacc tcgcgcgacg cgtacgatcg cggcgaaggg cgctaagcct tcgtactcgg
     6361 tcgatcgctc acgcgcttgt gccacgcggc gagattcggg tgcgtctccg ctttgatgcg
     6421 gatgtcgctg accttgccga agtcgatcgc gcacagcgcg gtgatgtcgg cgaccgtgaa
     6481 acgatcgccg gccatgtact ggcgcttgcc gagctcgcca tcgagccagt cgaagcgcag
     6541 gccgatgtgc tcgcgcatga tcccggcgaa ctcgggcgcc tgcttgatgc ggccgaccca
     6601 gaacggatgc gtgttgcgga acacttgaga gatcggccac aggagctcga gctcggcgtg
     6661 acgattccac atctcgacct gtgcgcgttc ccacgcgtcg gcgccgaaca agttcggctc
     6721 ggggtgcagc tcttcgatgt atctggagat ggccatcgac tcgcgtagga tccggccatc
     6781 gggaaattcc agaaccggta gcagcgaaat cgggttcttg cgccggaact cgtccgtctg
     6841 gtgcgccgaa ttcgcgatca gaacctcgat cgtgtcgtac gccacgctct tctcggatag
     6901 gaagatgcgc acccggcggg ggttgggggc gcgggggtcg tggtacagca gcatgccggc
     6961 gactgtacgg ccaaaaaacg gcatgcgtcg cgcggatctc gtacaatcgg gaagtatgcg
     7021 catcgcgggg atcctggtcg tcctgtgcgt cttcctgtcg cttggcgcgg ccacgggctg
     7081 caagaacaag ggtgacgccg acgcggcgcc agatccggag gccgtcaaag ctcaacagga
     7141 gctgatcgct cgccgcgaca agctgctcga tgcgcgcaag aagctgcagt ccgacaagga
     7201 caagctcgac gccgagatca aggagatcca ggcgaagggc ggcgatgcga ccgagcagat
     7261 gaagaagaag gcagagctcg agaacgagct cgaatcttcg aacgagtcga tcatctcgct
     7321 cgtcaacagc aagctcgatg gcctcggcaa ggccgccgac aagaccgcgc aggtcgcgtc
     7381 ccgcgaagcc gaaatcgcgt cgcgggagaa agccgtcgcg gagcgcgagg cgaagctcgc
     7441 cgaccgcgaa cgccagctcg ctcagcgcga cagcgaatct gccgcacgct ggaaggagac
     7501 ctgctcggtc ggcgcgccga tgatcatcca ggcgacggcg cccaaagacg gcaagtacac
     7561 gaacaaggac gtgaccgcgc tgatcgcgaa ggcgaagaag gagatgaaca agaagaactt
     7621 gatcaactcc gatctcggcg ctgccgcatc gctcgagagc gaagccggca aggcgctcaa
     7681 cgagaacgac atgagcaagg cgtacttcgc agccgcgcag ctcgtcgcca cgatcgatgc
     7741 gatcacgatc aatcgccagt tcatccaggc gaaaaccgca cgcttgtccg cgcaggcgaa
     7801 gggcacgaag ctcgacgaag cgacgaacaa gcaaatcgcc gacggcctcg gcgacgtgat
     7861 gcagaagtac aacgacggcg acttcagcgc cgcgaaccgc cggctcaacc agctcgccag
     7921 catcctcgcg aagtccggcg gctgaccgcc gtcatcgtcg gcgagtgagt gcggggtagg
     7981 gcgggagctg cgcctacgca atcgtcgagc gcgagatcgt ccggccaaat cggtacgcgc
     8041 gttttaatac cggaacgggg ttagttttga cgcgcgtcaa gttccggtgt cgcctgcagt
     8101 ccggtgtgtc cgggccgcgc cgattgacgg acgcgctgtc cgacatcgcg cgtcgcagcc
     8161 gggcgatggt cgcgtcgtgc cgcagccggg gtgatgtcgc gtcgtgccgc agccgggcga
     8221 tcgcgtcgtg ccgcagccgg gaggattgtc gcgtcgtgcc gcagccgggc gatgtcgcgt
     8281 cgtgccgcag ccgggcgtcg cgtcgtgccg cagccgggac gattgtcgcg cagtgccgca
     8341 gccgggacga ttgtcgcgtc gtgccgcagc cgggcgattg tcgcgtcgtg ccgctgcggc
     8401 gggcggtatc atgacccgtg cgccgcgccg gactcctgct gctcgcgttg accggctgcg
     8461 cgaacatctt cgggctcgac tcgccgcaga tgcgcgacga cggcggagct ggtagcgaca
     8521 cgggcagcga cgggccgcca gtgtccgatt gccacagcga caacttcgac gacaacgcac
     8581 tgaatccagg gctatgggcg aagtttgtcg aaaagatgga gacggtcacc gagacaaacc
     8641 agcagctcgc gatcacgctc gacaacacga ctggcagcgc ctactccggc atcgacggcg
     8701 tactaccgct cgctgccgac gagaccggcg tgcaggtcga ggtcgtgcag tggagtggca
     8761 acgatcagag cgagacagcg ctggtcttgg ttctcaacgg cccgaaccag ctcatcatcg
     8821 gcaaggacgg caacagcttg cgtcagaccg tgaagacggg cggcgcggat atgacgcaca
     8881 tgtcgccgtt cgacgcgagc atccggttcc tccgcatcga gcgccgactc tcggacacca
     8941 aggtgacgtt cctgtcgagt accgatggca ccgcatggaa cttgttgttc atgacgaacg
     9001 cgtcgttcgc cgaccaagcg ctcaagccgc agctctacgc cggccactac cagcaggtgc
     9061 cggcgacgac ggtcatcttc gacaacttcg tcgtgttgtc gccgcgctgt ccgtagcggc
     9121 tagcagccca tgccgccgac cttgcgacag ttgtcgtagt cggggaagtc gtgcgcgatc
     9181 ttggcggcga ggaagccggc gccgatcgcg aggatcgcga tcaccgggcc agtgacgtac
     9241 gggcgccgcc acagcggcgg atccgtatag atgatcgtgt cgcgcgagcc cggcttctcc
     9301 tggatgtcgt gctcgacgat cgggtactgc ttcatcgtga cctggacgtc ggtggtcttg
     9361 ccgtaggtga cttcgacgaa cttcacgaag ttgtgatact gcggatgcgt cacggtgacc
     9421 gcgtgcgtgc cgacgggcag cgcgatgtct ttggttgccg atagctgcac cttggtgccg
     9481 ttgacgagca cggtcgcgcc gccgacgtcg aagccgaacc gcacgttgcc gcggtacttg
     9541 tcgggatcgg agagccgcac cgcggcgccc atcgggctgt cgctcacgcc gactgcgagc
     9601 gtggtcgaac gcagctcgcg ggccgcgccg acgtcgatga gcttgaggta gacgaccttc
     9661 gaatcgccga ggccaccgac ctcgccggtc acgacgaact gcgcgctgac cagcttgccg
     9721 acctcggtca cgcatccggc ttcgccttcg cactgcttga gcgccggctt ctttgccttg
     9781 tcgatcgccg cgctgacctg cgccgcgggc acgaccttgg tgccgggcag ggcggcgaac
     9841 gcttgctcga tctgcgtgac gagcttcttc gtcgcaaccg acttgtcctc ggcgccgagc
     9901 gtcgacagcg gcgtgatcgc gacgacgcgg tcggcgtaca ccgaggtggt cgcgagcagc
     9961 gtgagcaaga agacggcgcg gctcagcatg gcgtgctcgg atcgccgttg atgtaacagt
    10021 cgacgtgacc ggccgacgcg ccgatcagga ctgcgagcgc gacgacaccg acaccgaccg
    10081 cgccgatgaa ccacggccgc gtgtagatcg gcgagcgctc cttgtagatc gtcttgccgg
    10141 tgccgaccgg gccgctcgcc tggagcaagc gcaccacgac cgggctcacc ttctgaaagt
    10201 gcacctcgac atcgctgtcg aacgggtcgt agctcttcgc ttcgacgcgc aggcgatgtt
    10261 tgccgagcgg cagcttcgcg atcacgccgc cgtttgcgag cggcgtcttg ccgagcttgt
    10321 tgccgtcgag cgttacctcg gcgccgatca ggtcgctctg gatttgtagc gagccgtgga
    10381 tctgctgcgg tgcgagcagt cggtacgcgg cgacgcgcac accttcgatc aattcgtcgg
    10441 ggctgccgcg cagcgggtcg gactggatgc gctgcgactt gccggtcgcg acatcgacga
    10501 ccttgatgtt gaggacgtag ttgtcgccga gcgagccgac cgtgccggtc acgacccact
    10561 cgacgccgag ccgcttgccg atcgcagcca gacacttctc ttcgccggtg cagttctgct
    10621 ccgcgctcgt caccgaacgc tcgatgtcgc ggcggctcgg ctgcggcacc ttgtcgaggc
    10681 gatcgagctc catgcgaaac agcgtctcga ggcgctgcac gatctcgttg tcgatgccga
    10741 gcgcgtcgaa ccgccagacc gcgatcttct tgtcgagcag ctcgtccggt gcgggctgtg
    10801 cctgtgcacg cagcgcgacc gcaagtacga ccacaccgat gaccagcgag gctacccttg
    10861 ccaccttgag cagatcctat caccgcttcg ctaggattcg ccgtgttagc ctgcacacat
    10921 gtcttggctt cgcggggttg cgatcgcggc gtttgtcacg tcagcggtcg cctgttcctc
    10981 gatctcgacg gtccgggtgc agtccgagaa cgtgcatgtc gcgaaaggct tgcggcccat
    11041 cgccgtcgtc cacgcgcagg tgaccagctt cgagctgctc ttcttcgaga ttcccggcca
    11101 cgtcaccctc gactatgtcg tgaaccgcat gctgattcat gcggcgaagc agatgggagc
    11161 cgacaaggtc gtcgacatca aggtcgacat cacacccgac gacgggatct gggtcctgcg
    11221 ccggatcttc ggctggcggt ccgcgcaagc cagcggtgtc gcggtcgttg tcgaagacga
    11281 agcgcagtcg gcaccacccg ctgcaactcc tcccggccca cccgcgccga caccgagcaa
    11341 gtaggctata caccgcctgt gccgctggaa gtattcgacc gtcgcctgat cctcgtcgtc
    11401 ggcaaaggcg gcgtcgggcg cagcacggtc gctgcagcga tcgccggcaa gtgcgcggcc
    11461 gcgggacgac gcacgctgct gttcgaaacg aacgcgaacg atcgcttcgg tagctacttc
    11521 gataagccgc cggtcggcac cgagctgacc cagctcgcgc cgaacctgtg ggccgtcaac
    11581 acgaacccgg ccgcggcgct cgaagagtac ggactgatga tcctgaagtt caagtccgtt
    11641 tacgagatgg tcttcgagaa ccgagtgacg aaagcgttcc tgcgcgcgat tccgggtctc
    11701 gacgattact cgctgctcgg caaggcgtgg ttccacacga ccgaggagaa gcgcggcaag
    11761 ccgatctggg acaacatcgt cttcgacatg ccggcgtcgg gacactcggt gtcgatgctg
    11821 cgcatcccgt gggtgatcac ggacaccgtt ccggaaggtc cgctcacgcg cgacgcacgc
    11881 acggtgaaag agctgctctg cgatcgtgca cgcacggcga ccgtgctcgt gacactcgcg
    11941 gaagagatgc cggtcaacga agcgatcgag ctcgaagcga agctgaccga gctcggcatc
    12001 gtgccgcagc agatcgtctg caatcaggtc tacccgaatc acttcccgcc cggcgcgccg
    12061 gtgtctcgcg tgctcgacgc gctgacggcc gaccccacgc tgcagacgcc gctgcgcgaa
    12121 gtcgtcgagc acgcgacctt gtcgcgcgat cgccgcgcgc tcaacgagcg ttacctcgcg
    12181 gagctacgcc gtcgcgcgcg cacgccggtg cacgagctac ccatgttgtt cgcgccgacg
    12241 ctcgaggcgg cgcacgtcaa agccctcggc gatttgctgt agctactcgc ctgtcgcgcg
    12301 catcgggaac agcggctcgc cgtggatctc gaggcgataa acactgttct cggtctcgac
    12361 gtagatcatc ttgccgccgg gctcgccgag cacgcgcttc actggcgtcg tgatcatgcg
    12421 gtgcccgtgc gggtcgcgga agatgaccat gccgcgcccg atgtacgggt acgagatcaa
    12481 ataacctgta taaattcgtc cgctggcccc accgggtcgc tcggagccaa gctcgcgaat
    12541 cttccgtgca gtgacgctga ccatggaagc gctacctatt agtctaagag atacacatgg
    12601 ccgcgtcatc ctgtttggag gtcagccatg agtgacccga gcgttgaagc gctgtacgag
    12661 caggtgcgca cagctgtacc caccgcacct ggggagatgc ctgacgcact gctcccggtt
    12721 gcgccgggga ttcgcgtgct tgcgcttcgg acgccgaccc tcccgccggc ggcgcacacg
    12781 aacgtctacg tggtcggacc cgagcgcggg ccacaggtcg cgatcgatcc gggatcgccg
    12841 tacgccgatc aacagagttt gttagcgaaa gcgatctcgc aggaagcgga tgcggggagg
    12901 ccgctcgcgt gcgtgctgct gacgcatcac cacggcgatc acgtgggtgg cgcggtcgct
    12961 ctggcgacgg cgttcggcgt gccgatcgca gcgcacgcga acacggcgcg ccggctcgcg
    13021 ggccgtgttc cggtcacacg cgagctggtc gatcgcgaag agctctacgg cgtcacctgc
    13081 atctacacgc cggggcatgc cgacgggcat ctctgctacg agcacggtga ggcatcggtc
    13141 gtgggcgaca tggtcgccgg gctgggcacg atcttgatcg atcccatcga gggcaacatg
    13201 gcccactacc tcggctcgct cgccgcgctg ctcgagcgcc cgacgcgccg gctcctgcct
    13261 gcccatggcc cgatgatcga ggacggccct agcaagctgc gcgaatacat cgcacaccgc
    13321 acgatgcgcg aggaccgcgt gctggccgca ctgaagggca aaaccgacgc gtccgtcgag
    13381 gacctcgtgc cgatcgcgta cgccgacacg ccaaagctgc tctggccgct tgcagagcgt
    13441 tctttgcgtg cacatctcga caagctcgta ggggaaggcc gcgcgcgcga ggtcggcccc
    13501 gagcgatgga cggcagggta atccccgcta tagtcgcgcc ttggtggtac gcacgttcga
    13561 tacgttgccg gaggtcacgc tcgcccgcga tctattgcga cgctggtatg gcctcgagct
    13621 cggttacgcc gcgggtgatg gcagcggcta cgagcgcacg tcgcatgcgg cctgcgcgac
    13681 gctgctcgcg catccgagcg gtgcgtgcgg cgcgcagatg aaagacgtcg cggcgcagct
    13741 cgccgcgaag aaggacggca acgcgatcgt ccagcgctgc tatgcgggca tgacgatcgt
    13801 cgcggcgccc gtgcgcgaca ccaacgcgct cgcaggcatc gtttacgcga gtggcggccg
    13861 taccggcgca gtcgccgatg tcgaagctgc gatcgcgaag actaccggcg ccaccggcga
    13921 agcagccacc ctcgcggcgc tcgctccgga gctcggcgat gccgacatcg cgcgggtgaa
    13981 ggatctcgtg aagtctgctg cggcggcagt cgagcgctcg cagcccgcga cgattccgcc
    14041 gaccacctgg acccatccat tcaacgagat cgtcggcgac gcgcccgcgg tgcgtgacgt
    14101 gatcaagctg ctgaacaaag tcgtgaagag cgaggcgacc gtcctcgtgc acggcgagag
    14161 cgggaccggc aaggagctga tcgcgcgcgc gatccactat cacgggccac gcgcgaagaa
    14221 ggcgttcgtc gtccagaact gctccgcatt caacgacaac ctgctcgaga gcgcgctgtt
    14281 cggtcacgtg cgcggtgcgt tcacgggtgc cgtcaaagat caaaagggtc tgttcgaaac
    14341 cgccgacggc ggcacgttct tcctcgacga gatcggcgac atgtccgccg cgctgcaggt
    14401 caagctcctg cgcgtgctcc aagagggcac gttcatgccg gtcggcggca cgaagccggt
    14461 caaggtcgac gtccgcatca tcgccgcgtc gcacaagaac ctggccacca tggtccagca
    14521 gaagcagttc cgcgaagatt tgtttttccg cgtcaacgtc ctgccgatca cggtgccgcc
    14581 gctgcgcgac cgcgtcgccg acattccgcg cctcgcgcag cacttcgtga cgaagcactg
    14641 gcgcaatccc ggcgccgcgc cgaagctgtc gcagagtgcg ctcgcacgtc tcgtcgccta
    14701 cccatggccg ggcaacatcc gcgagctcga gaacgagatc gagcgcatgc tcgtgctcgc
    14761 cggcgatgcc caggagctgg tcggctcgat ggtctcctca cgcgtcgcgg gtgcaggcaa
    14821 cgcgggccgc ccgctgcgcg acatcgtcga cgtcgccgag gccgacgcga tcgttgcggc
    14881 gctcggccgc cacagtggcg atcgcgacgc cgcttccgcc gagctcaacg tgtcgcgcgc
    14941 ctacctcgac gcgcgctgta cggcgctcgg gctatgacga agcagacgaa gggcaagtgc
    15001 ccgagctgtc gcggcgaagc gatcaaggaa ggcaacaagc tctggccgtt ctgcagcgag
    15061 cgctgccatc tcatcgatct gggccgctgg ctcgacgagg agtaccgcgt gcccgacgag
    15121 cccgacgcct cgggcggcgg catcgagtcc gtgacgccgc acaccaaaga cgacaactag
    15181 cggcccacct gccgctttgt tcctcgttga acgcggtcga cccgctgcta cgctcatcgc
    15241 atgaccggcg tgccactcgg gatcatgctc ggtgcgctca acgttgtcgt catcgcgatc
    15301 ggcatggcgg gcatcggtgg cgaatccggc atcgcgatgt gggtcgtgat gttcggcatc
    15361 gtgccgggcc tcgtgatcgg cgcgctgctc ggctggctcg ccgaggtgat gaagccgctg
    15421 ccgatctggg tgcggcgctt cgtgctgatc gtgcccgcgg tgctgctcgt cgtggtgctc
    15481 gcagccgagt tctcgctcga aggattcatc ttgctgtcgt gcatcccgac cgcggtcgcg
    15541 acgtttctcc tcgagcgcgg cacgcgccaa gtcgcgccgc cgccggtgcc ggtcgcgcac
    15601 gcgcgccgct cgtgaccgca gcggcgccgg cgctcacttg accgagccgc cgaaaaccca
    15661 gatcggtttc gtcgacttcg cgctcgcggt tgcaacgatg gcaaagcctt cgatgccgac
    15721 gaggtacatg cccggcttga gaccctgcgc catcactttg ccgacctcga gcggctcggc
    15781 ggtgaggtcc atcttcacga tgccccactt caggcactcg cggcgataga gcggtgagcc
    15841 gtcagcgcgg aacgtttcga tgtggtccgt ctccttccac ttgatgcagt cctcgcgcgg
    15901 ctcgacaacg cgcttgaagg agatcacgac gctaccttcg ttctcctcga tcttcgcgat
    15961 cacgccctgg cgctgctgct ggaaccgcgc cccctccgac atcggtaggt ttccgttcgc
    16021 gagcaactcg ttcatcttca ggccgcggtc gtcgaacttg atctgtgcgg acgatgccat
    16081 ccacgcggcg atcgtcgccg tgcgcggtcc gcgttgcacg acgtcggcgc cgacgatgtc
    16141 gaggcccttg gaggaattct cgtcacccgc ggagcacagc cggagcgcct gatacgcgag
    16201 atacccttcg gggctgccga gcgtcgcggc aacgaacgcc gacgccgcac cttccgaggg
    16261 caccttggga agcttgagac ccttgagctg cgcctcccac gccgcacgcg tgctcgcatc
    16321 acaaccggcg aaggcggagt gcttgttcga tcgggtcgcg gcttccatgg tctcgagttg
    16381 cgcgacgagc ttggcacgcg ccgggctggg cgacgcccac tccttg
//
LOCUS       DASUFX010000005         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1389659, whole genome shotgun sequence.
ACCESSION   DASUFX010000005 DASUFX010000000
VERSION     DASUFX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389659"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFV99_00190"
     CDS             complement(<1..719)
                     /locus_tag="VFV99_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057736.1"
                     /translation="MPLAGSIKLRSFLAIIVGIGCAGVFLLFKACKSSEPPPKPADVV
                     VKPKPEPTPTPTPPEPTPQQPSGQEMAARAWDADVIAWSKKTISGDKQKDVSKKKPYK
                     INVYREAGHATVNRAKVDINRNDKWDEKYTFDGDTITLEIAPADDENYTKTYHWTGSA
                     WLPEGATPTAEQGSAPNPDTAAKPDTPKPDNGGAKIDLPARTYDAEVIAWKDKSIASD
                     KEKDVSKGKSYKINVYKDAGQT"
     gene            complement(719..1561)
                     /locus_tag="VFV99_00195"
     CDS             complement(719..1561)
                     /locus_tag="VFV99_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5057737.1"
                     /translation="MITVPRYPLEGVVSWNMNTTCNYRCSYCTQRFVDDRGQWAKDLP
                     RFIAAFAALPGDWEIKLSGGEPFRHPDFLDAVAKLAAARRRVSVVTNFSAPLDELFGF
                     ADVTRSKPGLISASLHLEYAEPDEFLAKVRAVQERHAGRVVVTCVATRDNLPRLQALR
                     AQFTAAGVTFRVQPEKQDRDVIDYDDSEREQLITLGGHNGTGLIAPSFAGQPCWAGAR
                     YFIVDHQGDAYRCYPARRQRKERLGNLLDGTFRIRMEPEPCRYAYCNCTVPQQRGMVE
                     VRRS"
     gene            complement(1558..>2470)
                     /locus_tag="VFV99_00200"
     CDS             complement(1558..>2470)
                     /locus_tag="VFV99_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057738.1"
                     /translation="GCRVARGHRRGAREPPASDRMTRTRTIEWQVAGACNYDCSYCIQ
                     SSRYRRGRPQRDQLARAIDAFAALPGRWEIKCSGGEAFAHQLFMTYVVPELMAKTPHA
                     ISVLTNFSATRAELMQFAQLTRGRLAVFSASLHLEFTTVEEFAAKAEWFVLQLEPSVS
                     FVVNQVVLPGREHEAEACRAAIEARGLRWFPQLYKHDGGVHDYDNPELLVPLGIGLGR
                     RATPREANTAPSYRGLTCWAGVDYFVVDKNGDAWACRTAKRFGEGALGNIFDGTLALH
                     AEPAPCSYDICPCTVPANRGMIEGIAG"
BASE COUNT          370 a          787 c          843 g          470 t
ORIGIN      
        1 gtctgccccg cgtccttgta gacgttgatc ttgtacgact tgcccttcga gacgtccttc
       61 tccttgtcgc tcgcgatcga cttgtccttc cacgcgatca cttcggcatc gtaggtgcgc
      121 gcgggcagat cgatcttcgc cccgccattg tcgggtttcg gcgtgtcggg cttcgctgcg
      181 gtatcggggt tcggcgcgct gccttgctcg gccgtcggtg tcgcgccttc gggcagccat
      241 gcgcttccgg tccagtggta ggtcttcgta tagttctcgt cgtcggcggg tgcgatctcg
      301 agtgtgatcg tgtcgccatc gaacgtgtac ttctcgtccc acttgtcgtt gcggttgatg
      361 tcgaccttcg cgcgattgac cgtcgcgtgc ccggcctcgc gatagacgtt gatcttgtac
      421 ggcttcttct tcgagacatc cttctgcttg tcgccgctga tcgtcttctt gctccacgcg
      481 atcacgtcgg catcccaggc gcgcgcggcc atctcctggc cggacggctg ctggggcgtc
      541 ggctcgggcg gggttggcgt cggcgtcggc tctggcttcg gcttgacgac gacgtcagcc
      601 ggcttcggcg gcggctcgct cgacttgcac gccttgaaca gcaggaacac tccggcgcac
      661 ccgatgccaa cgatgatggc gagaaacgaa cgcagcttga tcgaaccagc gagcggcatc
      721 aggacctccg tacttcgacc atgccgcgtt gctgcggcac ggtgcagtta caatacgcat
      781 agcgacacgg ttcgggttcc atccgaatgc gaaacgttcc gtcgagcagg ttgccgaggc
      841 gctctttgcg ttgccggcgg gcggggtagc agcgatatgc gtcgccctga tgatcgacga
      901 tgaagtagcg cgcgcctgcc cagcacggct gaccggcgaa gctcggggcg atcagaccgg
      961 tgccgttgtg gccgccaagc gtgatgagct gctcgcgctc gctgtcgtcg tagtcgatga
     1021 cgtcgcgatc ctgcttttcg ggttgcacgc gaaacgtgac gccggccgcg gtgaactgag
     1081 cgcgcaacgc ctgcaggcga ggcaggttgt cgcgcgtcgc gacgcacgtg acgacgacgc
     1141 ggccggcgtg gcgctcctga actgcgcgca cctttgccag aaactcgtcg ggctctgcgt
     1201 actcgaggtg gagcgacgcg gagatcaggc cgggcttgct gcgcgtgacg tcggcaaagc
     1261 cgaacagctc gtcgagcggt gccgagaagt tcgtgacgac gctgacccgg cggcgcgccg
     1321 cggcgagctt ggcgactgcg tcgaggaaat cgggatggcg gaacggctcg ccgcccgaga
     1381 gcttgatctc ccagtcgccg ggcagtgcgg cgaatgcggc gatgaagcgc ggcagatcct
     1441 tcgcccactg accgcgatcg tcgacgaacc gctgcgtgca gtagctgcaa cggtagttgc
     1501 aggtcgtgtt catgttccac gacaccacgc cttcgagcgg atagcgcggc acggtgatca
     1561 tccggcgatc ccttcgatca tgccgcggtt cgccggcacc gtgcacgggc agatgtcgta
     1621 gctgcatggc gcgggttcgg catggagagc gagcgtgccg tcgaagatat tgccgagcgc
     1681 gccttcaccg aagcgctttg cggtgcggca cgcccacgcg tcgccgttct tgtcgacgac
     1741 gaagtaatcg acgcctgccc agcaggtgag gccgcggtag ctcggtgcgg tgtttgcttc
     1801 gcgtggcgtg gcgcggcggc cgagcccgat gccgagcggc acgagcagct cggggttgtc
     1861 atagtcgtga acgccgccgt cgtgcttgta gagctgcggg aaccagcgca agccgcgggc
     1921 ttcgatcgcg gcgcggcacg cttcggcttc gtgctcgcgt ccgggcaaca cgacctggtt
     1981 gacgacgaag ctgacgctcg gttcgagctg gagcacgaac cactcggcct tcgcggcgaa
     2041 ttcctcgacg gtcgtgaact cgagatggag cgacgccgag aacacggcga gccggccgcg
     2101 cgtgagctgc gcgaactgca tcagttcggc gcgcgtcgcg gagaagttcg tcagcacgct
     2161 gatcgcatgc ggcgtcttcg ccatcagctc gggcaccacg tacgtcatga acagctgatg
     2221 cgcgaacgct tcgccgccgg agcacttgat ctcccagcgg ccgggcaggg cggcgaacgc
     2281 gtcgatcgca cgtgcgagct ggtcgcgttg tggacgaccg cgacgataac gggacgactg
     2341 gatgcaatac gaacagtcgt agttgcacgc gccggcgacc tgccactcga tcgtccgcgt
     2401 gcgcgtcatc cgatcactcg caggtggttc acgcgcgccg cgccggtgac ctcgcgccac
     2461 gcggcatccc
//
LOCUS       DASUFX010000006         7453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_417574,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000006 DASUFX010000000
VERSION     DASUFX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417574"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFV99_00205"
     CDS             complement(<1..394)
                     /locus_tag="VFV99_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057739.1"
                     /translation="MRRGFRDSAEPALDEGDVVASAYHVRREITRVHTGAVFEAHDTM
                     LDRLVALKLAWRDPGTPSLVTEARRCAALRDPCSVQVHGMGLYDGIEYAVAERVIGKL
                     LRDDHDRTLMPELYLARLRRLVAAVARAH"
     gene            complement(412..1263)
                     /locus_tag="VFV99_00210"
     CDS             complement(412..1263)
                     /locus_tag="VFV99_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057740.1"
                     /translation="MIIALVLFSALLHALWNALLRIEKDKDRTLVAAVCVATLVSALV
                     AGVRLGLGQVAFATTLSFVWAVVAGVLEWLYFASLAKALDRGSLGPVYTISRGGAIVL
                     VWPLSIVVFDEPLTVLSAVGSGVVFGGLALASSRTGDRRKTDGSALAWASACAVAISG
                     YHLAYKAALRDGGGASAVFTVGLAVASLINLVRIGSEGRTVVATLLRTRLPRIVLMGI
                     VSAASFLILIEALAAGGAGYVLTLRNTSVLFATGLAFAIGERPRRVEILGAACVAAGA
                     VLMAWPP"
     gene            complement(1306..2070)
                     /locus_tag="VFV99_00215"
     CDS             complement(1306..2070)
                     /locus_tag="VFV99_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5057741.1"
                     /translation="MKKHLIRVGNSYALIIEKSIRNLLYLGPRSILEVSTDGTRILIE
                     PTGQLLQDDEIGRARELLSAEIGMPRPTSPDGLSRKALDIDARGIWTELERRYYIDPQ
                     RLARLHHEPVGTTRPISRLMRVGSWMTEPNCATRANDEELAFLRRMRVCRDRLREGAT
                     WDEAIDDAVRAFPKGDATVTSGATMTAATIQAEATTLPARAAPQPQASDALVTDARVQ
                     APGTSDALVTDGRVRALETSDALVTDGPVRATAPPV"
     gene            complement(2664..3671)
                     /locus_tag="VFV99_00220"
     CDS             complement(2664..3671)
                     /locus_tag="VFV99_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5057742.1"
                     /translation="MAADLYGIPWPTAPSLAQTQRDEIALEGGAKLYHFRGARTSIAS
                     ATGKPLLLIPSLINRWYVLDLRPGASLVEALVRAGIDVWCLDWGVPEAEDRYLDWEAV
                     LARLGRAVRRVKRETAADKLAVLGYCMGGTLTTIYAAQHASDFAALITLATPIDFQRG
                     GQLRCMVEPQWFDVDAIADAGNVAPVQMQAGFVALRPTLDLGKIMAMPDLATDARARE
                     AFFALDEWASDNIPFPAEAYRRYIRELYQENSLVEGTHRVAGRAVSLGAIQCPTLVIT
                     ASRDAICPPAAATALLSHISATDTHVLEVPGGHVGAVVGSKAAKEMYPALIRWLQPRI
                     A"
     gene            complement(3726..4052)
                     /locus_tag="VFV99_00225"
     CDS             complement(3726..4052)
                     /locus_tag="VFV99_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057743.1"
                     /translation="MSKEPESKTIPPRTEAAAPHNPFSAFDPMAAWTAGQATWQKFVT
                     DAFARTQSWADEYASIEAQMYGRALQAVDTWAQLARDTIQYSQNLSIQARKLGFEAAR
                     KAGIGA"
     gene            4193..5548
                     /locus_tag="VFV99_00230"
     CDS             4193..5548
                     /locus_tag="VFV99_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5057744.1"
                     /translation="MNNEKPWWKEPTRQQWAAFSAAWVGWVLDAFDFMVYVLVMRQLA
                     AEFGVGLAAVSGSITLTLLVRLAGGAIAGWMADRWGRKLPLILALVWFVSFDAAIYFS
                     HSFTAILIFRTLFGFGMGAQWTAGTALAMESMPERTRKIGSGLLQAGWPIGVMLAAGA
                     AYFIVDAHTWRPMFLVGIIPAALTIPLWFMFPSTKPAPKALGAKNESPLRALASPGVP
                     KMLVLGSLVMTLGFIVYYGLQSQYPAMLQGELHLSSQSAFLHVILFNAGMLFGVIAAG
                     FVAQRFGVIAALVAPALLMVPALPLYVGFVPVALPIGAFLGGALGVGYSGVTPVLTTS
                     LFPAHVRALAIGLVYHVGAFFAAFVPWLIPQIATHFHLKLSTVLMIVVGTGLIAMSTA
                     VLLLRKMITIPAEEPKPTGFDVDTAPVETPNFRPAPLPVAHTVADREVAKAAERRAIL
                     G"
     gene            complement(5618..6277)
                     /locus_tag="VFV99_00235"
     CDS             complement(5618..6277)
                     /locus_tag="VFV99_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEX5057745.1"
                     /translation="MVLIKKYGNRRLYDTGDSRYVTLDELAGKIRSGSDLRVVDAQTG
                     EDLTQATLTQIVLETGNAAKFLPVQLLTQMIRLSDDALAEFFSRYVTGALDLYLQAKR
                     GVQTIATYNPLTQIPMAAGDALARMWMGGFGGGAPYQQYPGYTAPPPYPVPAPEDETL
                     EEDSPTRKRPKTEPEKRAKTEPPAGNHDRADDVAAMRRELEELKQVLKDNLPKRKKRK
                     P"
     gene            6387..7283
                     /locus_tag="VFV99_00240"
     CDS             6387..7283
                     /locus_tag="VFV99_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057746.1"
                     /translation="MLSRAHGTFLLLCSMSVACGSPSGETCTLDSDCASGFCKADGTC
                     GPAPVDAKPESDGDVDGMIGLCNPNHDGMITLAELPFMAGRMGTFRVTTDVTWDTAGV
                     SGANGSRTWDLSGALAGDADRMMMLGAPTAQWWSADYPTATYATELSIESDLKGVFKV
                     TGAESTLQAVVSPAGGTYKTELDYDQPAQILKLPLTAGATWTSTSTVSGYAQGVIAAY
                     TEKYESRVDQVGTMKTPYGEFPVLRVATDLTRTSGFTTLLTKRTFSWVAECFGPVATA
                     ASQDFATGAEFTDNAEIRRLAP"
BASE COUNT         1230 a         2453 c         2420 g         1350 t
ORIGIN      
        1 cgtgcgcacg tgcgaccgcg gcaacgaggc gacgcagccg cgcgagatag agctccggca
       61 tcagcgtgcg atcgtgatcg tcgcgcagca gcttgccgat gacgcgctcg gcaacggcat
      121 actcgatgcc gtcgtacagc cccatcccat gcacctggac ggagcatgga tcgcgcagcg
      181 ccgcacaccg ccgcgcttcg gtgacgagtg acggcgtccc cggatcgcgc caggcgagct
      241 tcagcgcgac gagccgatcg agcatcgtgt catgcgcttc gaacaccgca ccggtgtgca
      301 cgcgcgtgat ctcgcggcgc acgtgatacg cggaggcgac gacatcgcct tcgtctagcg
      361 cgggctcggc actgtcacga aaaccgcgtc tcatccccgt ctacaacgct atcacggcgg
      421 ccatgccatc aggacggctc ctgctgcgac gcacgccgcg ccgagaatct cgacgcgacg
      481 aggccgttcg ccgatcgcga acgcgagccc ggttgcgaat aacaccgacg tgttgcgaag
      541 tgtgagcacg tagcctgcgc cgccggcagc cagcgcttcg atcaagatca agaacgaggc
      601 agcggacacg atgcccatca gcacgatccg aggtagccgc gtgcgcagca gcgtcgcgac
      661 gaccgtgcgg ccctcgctgc caattcggac gaggttgatg agcgacgcga ccgcgagccc
      721 taccgtgaac accgccgatg caccgccgcc gtcgcgcagc gccgccttgt aagcgaggtg
      781 gtaacccgaa atcgccactg cgcatgcact cgcccatgcg agcgcgctgc cgtcggtctt
      841 gcgccgatcg cccgtgcgcg agcttgcgag cgcgagcccg ccgaacacga caccgctacc
      901 gacggccgat agcaccgtga gcggctcgtc gaacacgacg atcgagagcg gccagaccag
      961 cacgatcgcg ccgccgcgcg agatcgtgta gacggggccg agcgaaccgc ggtcgagcgc
     1021 tttggcgagc gatgcgaagt acagccactc gagtacgcca gcgacgaccg cccacacgaa
     1081 cgagagcgtc gtcgcgaacg cgacctggcc aaggccgagc cgcacgccgg cgacaagcgc
     1141 ggacacgagt gtggcgacgc acacggcggc gacgagcgtg cggtccttgt ccttctcgat
     1201 tcgcagcagc gcattccaca acgcgtggag cagcgccgag aacagaacga gcgcgatgat
     1261 caagccggcc gagcgtaccg gttcattcgc gctgcgccca gcaccctaca caggaggtgc
     1321 agttgcgcgt accggtccgt ccgtcactag tgcatccgaa gtctccagtg ctcgcacccg
     1381 tccgtccgtc accagtgcgt ccgaagtccc cggtgcttgt acccgtgcgt ccgtcactag
     1441 tgcgtccgaa gcctgcggtt gcggcgctgc gcgcgcgggc agagtggttg cttcggcctg
     1501 aatcgttgcg gctgtcattg tcgctcctga agtgacggtc gcatcgccct tgggaaaagc
     1561 gcgcaccgca tcgtcgattg cttcgtccca cgtcgcgcct tcgcgcaatc gatcgcgaca
     1621 taccctcatg cgccgcagaa acgcgagctc ctcgtcgttt gcacgagtcg cgcagttggg
     1681 ctcggtcatc caactcccta cccgcatgag acgagaaatc ggccgcgtcg tcccaaccgg
     1741 ctcgtgatgc agccgtgcaa gtcgctgtgg atcgatgtag tacctgcgtt cgagctcggt
     1801 ccagatccca cgtgcgtcga tatcaagggc cttgcgggag aggccatctg gcgacgtcgg
     1861 acgcggcatg ccgatctcgg cggagagcag ctcacgcgca cgcccgattt cgtcgtcttg
     1921 gaggagctgc ccggtcggct cgatcaggat gcgcgttccg tcggtactga cttccagaat
     1981 tgatcgcgga ccgaggtaga gcaggtttcg tatcgacttc tcgatgatca gtgcgtagct
     2041 gttaccgacg cgaatgaggt gcttcttcat cagacttcgt tacgccacga ggctgacact
     2101 cgtggatgaa ggcgccgact acgttcagga agcagcgtcc gatgaccgga cagcagacgc
     2161 gccagctacc aactctgttg ggtatacata agtagggttc tgcgacgatc tggttagtac
     2221 ggcgttatga ccgcggtgtg attagagagc cggaccatgt gcagggtctc ctgccacgat
     2281 ccggttagta cgccgttatg acggcggccg cttcgcaccg gcgcatatat gcagggtctc
     2341 ttgccgcgat ccggttagta cgtcgttatg acggcggccg cttcgaaccg ccgcatatat
     2401 gcagggtctc ttgccgcgat ccggttagta cggcgtttat gacggcggcc gcccgtagtc
     2461 gctctcttcg gctcgagtac gccagcgtac taacgctgta cttaccagat cgcgatggga
     2521 cacccttcac atatagggat ctcctcaacg ggcgcgctcg gcggccgcaa cccgcagcca
     2581 gagcaggcgc gcttcggacc taaaaggggc gcagcccgcg ccaccatttt gcgggatgga
     2641 ggtggcggcg cgggcgcgca atctcaagcg atgcgaggct gcagccagcg aatgagcgcc
     2701 ggatacatct ccttggccgc cttgctgccg acgaccgcgc cgacgtgccc gcccggtact
     2761 tcgaggacgt gcgtatcggt ggcgctgatg tgactcagca gtgcggtcgc tgctgccggt
     2821 ggacagatgg catcgcggct cgccgtgatg acgagcgtcg gacattggat ggcgccgagc
     2881 gaaaccgcgc ggcccgcgac acgatgcgtg ccttcgacga gtgaattctc ttggtagagc
     2941 tcgcggatgt atcggcgata cgcttccgcg gggaagggaa tgttgtcact cgcccactcg
     3001 tcgagggcaa agaacgcttc gcgtgcgcgc gcgtccgtgg cgagatctgg catcgccatg
     3061 atcttgccga ggtcgagcgt cggcctcagc gcgacgaagc ccgcttgcat ttgcaccgga
     3121 gccacgttgc ctgcgtcggc gatcgcatcg acatcgaacc attgcggctc gaccatgcag
     3181 cgcagctgac cgccgcgctg aaagtcgatc ggcgtcgcta gcgtgatcaa tgcggcgaag
     3241 tcactcgcgt gttgcgccgc atagatcgtc gttagcgtgc cacccatgca gtagccgagc
     3301 acggcgagct tgtcggccgc tgtctcgcgc ttcacacgtc gtacggcgcg gccgaggcgc
     3361 gcgaggaccg cttcccaatc gagatagcga tcttcggctt cgggcacacc ccaatcgagg
     3421 caccacacgt cgattccggc gcggacaagc gcctcgacga gacttgcgcc cggccggagg
     3481 tcgagcacgt accaacgatt gatgagcgac gggatcagca acagcggctt gcccgtggcc
     3541 gaggcgatag acgtcctcgc accgcgaaag tggtacagct ttgcgccacc ttcgagcgcg
     3601 atctcgtcac gctgggtttg cgctaacgag ggggcggtcg gccacggaat gccgtagagg
     3661 tctgcggcca cgggggtcca cgcctctgca acctcacgtg gccaaccaaa cagctcgtgc
     3721 accgattacg ctccgatgcc ggccttgcgg gccgcctcga aaccaagctt gcgcgcctgg
     3781 atcgacaggt tctggctgta ctggatcgtg tcgcgcgcga gctgcgccca cgtgtcgacg
     3841 gcctgcagcg cgcggccgta catctgcgcc tcgatcgacg cgtactcgtc cgcccacgac
     3901 tgggtgcggg cgaacgcgtc ggtcacgaac ttctgccagg tggcctgacc ggcggtccag
     3961 gcggccatcg ggtcaaatgc gctgaacgga ttgtgcggtg cagcggcctc ggtacgcggg
     4021 ggaatggtct tggattcggg ttctttcgac atttgcagct cctttgcggc acaccctaag
     4081 gtcgacgccg catcatgtca atgacgattt gttgcgatgc aacaaaattt cctcctcgga
     4141 gtggtcattt ctctattgcg ccgcactaga acgatcagct actgaacccg acatgaacaa
     4201 cgagaagccc tggtggaaag agccgacgcg ccagcagtgg gccgcctttt cggcggcatg
     4261 ggtgggttgg gtcctcgatg cgttcgactt catggtctac gtgctcgtga tgcggcagct
     4321 cgccgccgag ttcggcgtcg gcctcgcagc ggtgtcgggc tcgatcacgc tcacgttgct
     4381 cgtgcgcctc gcgggcggcg cgattgcggg ctggatggcg gaccgctggg gccgcaagct
     4441 tcctctgatc ctcgcgctgg tgtggttcgt cagcttcgac gcggcgatct acttctcgca
     4501 ctcgttcacg gcgatcctca tcttccgcac gttgttcggt ttcgggatgg gtgcgcaatg
     4561 gacggccggc accgcgcttg cgatggagag catgccggag cgcacgcgta agatcggctc
     4621 tggccttctg caggccggct ggccgatcgg tgtgatgctc gcggccggcg ctgcgtactt
     4681 cattgtcgac gcgcacacgt ggcgaccgat gttcctcgtc gggatcatcc cggcggcgct
     4741 cacgatcccg ctgtggttca tgttcccgtc gaccaagccg gcaccgaagg cactcggtgc
     4801 aaagaacgag tcgccgctcc gcgcactcgc gagccccggc gtgccgaaga tgctcgtcct
     4861 cggctcgctc gtgatgacgc tcggcttcat cgtctattac ggcctgcagt cgcagtaccc
     4921 ggcgatgctc cagggcgagc tgcacctgtc gtcacagagc gcgttcctcc acgtcatcct
     4981 gttcaacgcc ggcatgctgt tcggtgtcat cgcggccggc ttcgtcgcac agcgcttcgg
     5041 tgtgatcgcc gcgctcgtcg ctcccgcgct actgatggtg ccggcactgc cgctgtatgt
     5101 cggtttcgta ccagtggcgc tgccgatcgg cgcgttcctc gggggcgcac tcggcgtcgg
     5161 ctactcgggt gtgaccccgg tgctgacgac gagcctgttc cccgcccatg tgcgtgcgct
     5221 cgcgatcggc ctcgtctatc acgtcggtgc gttcttcgcg gcgttcgtgc cgtggctgat
     5281 cccgcagatc gccacccact ttcacctcaa gctgtcgacc gtgctcatga tcgtcgtcgg
     5341 tacagggctg atcgcgatga gcaccgccgt tttgctgctg cgcaagatga tcaccatccc
     5401 ggccgaggag ccgaagccca cgggattcga cgtcgacacc gcgcccgtcg agacgccgaa
     5461 tttccggccc gcgccgctgc cggtcgcgca cacggtcgcc gatcgcgagg tcgcgaaggc
     5521 tgccgagcgt cgcgcgatcc tcggttagct tcgctacgtc gtcgctgcac agttcgcgca
     5581 acgtcgcggc ctgtgatgcg atgaccgggc cgaggagcta cggcttccgc ttcttgcgct
     5641 tcggcaggtt gtccttgagc acctgcttca gctcttcgag ctcgcgccgc atcgccgcga
     5701 catcgtctgc gcgatcgtga ttgccggcgg gcggctcggt cttcgcgcgc ttctcgggct
     5761 cggtcttcgg ccgcttgcgc gtcggactgt cttcctcgag cgtctcgtct tcgggcgctg
     5821 gcactggata gggcggcggt gcggtgtagc ccggatactg ctgatagggc gctccaccgc
     5881 caaagccacc catccacatc cgtgcgagcg catcgcccgc ggccatcggg atctgcgtca
     5941 gcgggttgta cgttgcgatc gtctgcacac cgcgcttggc ctgcaggtag agatcgagcg
     6001 cgccggtgac atagcgcgaa aagaattcgg cgagcgcgtc atcggagagc cggatcatct
     6061 gcgtcagtag ttgcaccggc aagaacttcg ccgcgttccc ggtctcgagc acgatctgcg
     6121 tcagcgttgc ctgcgtcagg tcctcgccgg tttgcgcatc gacgacgcgg agatcggaac
     6181 cgctccggat cttccccgcg agctcgtcga gcgtcacgta ccggctatcg cccgtgtcgt
     6241 agagccgccg gttgccgtac ttcttgatca ggaccatcgc cactggggca caccttagag
     6301 gctcgttcgc tgcactgcaa gcaggacttt tgctgcaccg caaaagaacg cttgacacct
     6361 tctgcacagc cacgtagcgt gcgcccatgt tgtcgcgggc ccatggcaca ttcttgttgc
     6421 tatgcagcat gagtgtcgca tgtggaagcc cttccggtga gacctgcacg ctcgacagcg
     6481 actgcgcgtc ggggttctgc aaagcagacg gcacgtgcgg tcccgcgccg gtcgatgcga
     6541 agccggagag cgatggcgat gtcgacggca tgatcggcct gtgcaatcca aaccacgacg
     6601 gcatgatcac gctcgccgag ctgccgttca tggccggtcg tatggggacg ttccgcgtca
     6661 ccaccgacgt gacctgggac accgccggcg tatcgggcgc gaacggctcg cgcacgtggg
     6721 acctcagcgg tgcgctcgcc ggcgatgccg accgcatgat gatgctcggt gcaccgaccg
     6781 cgcagtggtg gtcggctgac tatccgaccg cgacctacgc gaccgagctg tcgatcgagt
     6841 ccgatctcaa gggcgtgttc aaggtgacgg gcgccgagtc gacgctgcag gcggtcgtgt
     6901 cgccggccgg cggcacgtac aagacggagc tcgattacga ccagccggcg cagatcctca
     6961 agctgccgct caccgcgggc gcgacgtgga cgtcgacgag cacggtgtcg ggctacgcgc
     7021 agggcgtgat cgccgcgtac accgagaaat atgaatcgcg cgtcgatcaa gtcggcacga
     7081 tgaagacgcc atacggcgaa ttcccggtgc tgcgcgtcgc gaccgacctg actcgcacgt
     7141 cgggcttcac gacgctgctg acgaagcgca cgttctcgtg ggtcgccgaa tgcttcgggc
     7201 cggtcgcaac cgccgcatcg caagatttcg cgacgggcgc cgagttcact gacaacgccg
     7261 agatccggag actcgcgccg tgatgcgctc ggcggttgga cgggtggctg cgctcgtcgc
     7321 tgccatcgtg ctcgcgggct gtccgagcta ccacgcggcg aagctaccgg gtacgccggc
     7381 cgacgcgacg ttcgttgccg tcgacgacgt gcaggtccac taccgcgaac aggggcaagg
     7441 cccagcggtc gtg
//
LOCUS       DASUFX010000007         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1529119, whole genome shotgun sequence.
ACCESSION   DASUFX010000007 DASUFX010000000
VERSION     DASUFX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529119"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFV99_00245"
     CDS             complement(<1..223)
                     /locus_tag="VFV99_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057747.1"
                     /translation="MLSLELGRRLQVPVVHLDQLRFRSGWQSVETAELLGHEQRALPR
                     DGRWIADGNNGATMETRLAAADTIIFLDLA"
     gene            complement(251..1135)
                     /locus_tag="VFV99_00250"
     CDS             complement(251..1135)
                     /locus_tag="VFV99_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5057748.1"
                     /translation="MGNNLRVVTLNFWGTEPPLERRIDLAVRQLRAIGADVVCMQEVR
                     PLDGVFGRTSADVIADQLDMYAQYAKSNEWAAGEGGMAKAGQEGLAIITRAPLTESRT
                     LALPEPRPADSRMMLSGLVATDGGPIWVHTTHLHYRLDDGVAREKQVLAIDAEIRSLG
                     REKDSAPQILCGDFNATPDSDEIRFLRGLTTLDGRRTHWQDAWLRLHRECQPGDGPNE
                     GITWSSENGFTRPLRSLDIDRRIDYVFVTSRKKDGRATIHASHVVMTERDGQGPDEIC
                     ASDHYGVMADVQVVPNPS"
     gene            complement(1125..1637)
                     /gene="queD"
                     /locus_tag="VFV99_00255"
     CDS             complement(1125..1637)
                     /gene="queD"
                     /locus_tag="VFV99_00255"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX5057749.1"
                     /translation="MPRKLIASDKPIVLTARAKAICQTRRSSLLKLEPRIRGTTDRRS
                     MLQTARMRTRLERSYRFEAAHFLPKVPPGHKCARMHGHSYLIEVVIEGEVDPERGWVM
                     DFADIDERAMPLIRQLDHQVLNEVDGLVNPTSELLAVWWWQRLSSVLVGLVEIVVSET
                     PTSRCVYRGQ"
     gene            1603..2724
                     /locus_tag="VFV99_00260"
     CDS             1603..2724
                     /locus_tag="VFV99_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5057750.1"
                     /translation="MGLSLAMSFLGIYVVETRGYPAWLLGIVFLAANLAQSLSNAWAG
                     NLSDRIGRRPLITGSLLVRSVFIAALGTQIMFDAPLWSLAINMVFTSALRGCFEPVAY
                     ALVADVCTSDQRIDAFGLQRMGTNLGWAIGPALGGTLTLVIPYGAVFYVAGAGMIIAA
                     IVTMRVADPMKTRTEPRSGELRAALVASWRSPRMRILLVGTFLAALLQTQMFSTFSVF
                     MTDELGLRKADVGYLYTINGICVLAFQIPAVALVRRLGIATALPWSSLINGLGFAVVG
                     LASGTAGGAVAMVAITVGEVLLAPAHQTAIAELADPSQRGRAYGVVGFAQMLGIAVAP
                     LVGGILFDLIGHHHAAMWSVIASIGIVQALCFAAFVRRA"
BASE COUNT          454 a          993 c          915 g          540 t
ORIGIN      
        1 tcgccagatc gagaaagatg atcgtatcgg cagcggcgag acgcgtctcc atcgtcgcgc
       61 cgttgttgcc gtcggcgatc cagcgtccgt cacgcggcag cgcacgctgc tcgtgcccca
      121 acagctccgc cgtctcgaca ctctgccagc cgctgcgaaa tcgaagctga tcgagatgca
      181 cgaccggcac ctgaagacgg cgtccgagct cgagcgacag cacccacgca gccgatgatc
      241 gcgatccgat tcacgacgga ttcggcacga cttgcacgtc ggccatcacg ccgtagtgat
      301 cgctcgcgca gatctcgtcg gggccctgcc catcgcgctc cgtcatcacg acgtgcgatg
      361 catgaatcgt ggcgcggccg tccttcttgc ggctcgtcac gaacacgtag tcgatgcggc
      421 gatcgatatc gagcgagcgc agcggacgcg tgaagccatt ctcgctcgac cacgtgatgc
      481 cttcgtttgg accatcgccg ggctgacact cgcgatgcag ccgcagccac gcgtcctgcc
      541 agtgcgtgcg cctgccgtcg agcgtggtca gtccgcgcag aaagcgaatc tcgtcggaat
      601 ccggcgtcgc gttgaagtcg ccgcacagga tctgcggcgc ggaatctttc tcgcggccaa
      661 gcgatcgaat ctcggcatcg atcgcgagca cctgcttctc gcgcgcgacg ccatcgtcga
      721 gccggtagtg cagatgcgtc gtgtgcaccc agatcggtcc gccatccgtg gcgacgagcc
      781 ccgacaacat catgcgcgaa tcagccggcc gcggctcggg cagcgcaagc gtgcgcgatt
      841 cggtgagcgg cgcgcgcgtg atgatcgcga ggccttcctg gccggctttc gccatgccgc
      901 cctcgcctgc cgcccactcg ttcgacttcg cgtactgcgc gtacatgtcg agctgatcgg
      961 cgatcacgtc cgcgctcgtg cgaccgaaca cgccgtccag cggccgcacc tcttgcatgc
     1021 acacgacgtc ggcgccgatc gcgcgcagct ggcgcacggc gagatcgatg cgccgctcga
     1081 gcggcggctc cgtgccccag aagttgagcg tgacgacgcg gagattattg cccacggtac
     1141 acgcaacgtg acgtgggcgt ttccgagacg acgatctcga ccagaccgac cagcacagat
     1201 gacagccgct gccaccacca caccgcgagt agttcgctcg tgggattaac caggccgtcg
     1261 acctcgttca gcacctgatg atcgagctgg cggatcagcg gcatcgcgcg ttcgtcgatg
     1321 tcagcgaaat ccatcaccca gccacgctct ggatcgacct caccctcgat caccacttcg
     1381 atcagatagc tgtgaccatg catgcgcgcg cacttgtggc cgggcggcac cttcggcagg
     1441 aagtgcgcgg cttcgaaacg ataagaccgt tccaaccgag tacgcatgcg cgcggtttgt
     1501 agcatgctac gtctgtccgt cgtgcctcga atacgcggtt ctagcttcag caagctggat
     1561 cgccgcgtct ggcagatggc cttcgcgcgc gccgtcaaca cgatgggctt gtcgctcgcg
     1621 atgagcttcc tcggcatcta tgtcgtcgag acgcgtggtt atccggcctg gctgttgggc
     1681 atcgtcttcc tcgcggcgaa cctcgcccag tcgctgtcga acgcgtgggc cggcaacctc
     1741 tccgaccgca tcggccgtcg cccgctcatc accggctcgc tgctcgtgcg gtcggtgttc
     1801 atcgcagcgc tcggcacgca gatcatgttc gacgcgccgc tgtggagcct cgcgatcaac
     1861 atggtgttca cgtccgctct gcgcggctgc ttcgagcccg tcgcctatgc gctcgtcgcc
     1921 gacgtctgca cgagcgatca acgcatcgat gcgttcggcc tccagcgcat gggcacgaac
     1981 ctcggctggg cgatcggtcc cgcactcggc ggcacgctca cgctcgtcat tccgtacggc
     2041 gccgtgttct acgtcgccgg cgccggcatg atcatcgcgg cgatcgtcac gatgcgcgtc
     2101 gccgatccga tgaagacgcg caccgagccg cgatctggcg agctgcgcgc cgcactcgtc
     2161 gcgtcgtggc gcagcccgcg catgcgcatt ctgctcgtcg gcacgttcct cgccgcattg
     2221 ctgcagacgc agatgttctc gacgttctcg gtcttcatga ccgacgagct cggtctgcgc
     2281 aaagccgacg tcgggtacct ctacacgatc aacggcatct gcgtgctcgc gtttcagatc
     2341 ccggccgttg cactcgtgcg cagactcggt atcgcgacag cgctgccgtg gtcatccctc
     2401 atcaatggcc tcggcttcgc ggtcgtcggt ctggcgagcg gcaccgcagg cggtgcggtc
     2461 gcgatggttg cgatcacggt cggcgaggtg ctgctcgcgc cagcgcacca gactgcgatc
     2521 gccgagctcg ccgatccgag ccagcgcggc cgcgcgtacg gcgtcgtcgg cttcgcgcag
     2581 atgctcggca ttgcagtggc accgctcgtc ggtggcatcc tgttcgacct gatcggtcac
     2641 caccatgcgg cgatgtggtc ggtcatcgcg agcatcggca tcgtgcaggc actttgcttc
     2701 gccgcgtttg tgcggcgcgc gtagacgaga tttcgcttta ccgtcaggga gaaagcgtta
     2761 gattcgcgcc cgatgagggc ttggattctc tgcgtcgcat tggcggcgtg tggcgacggt
     2821 acgccgtcga acatgctgcc tgcgtgggac aagacgctgc ctgcggcaag cgtgatgggc
     2881 accggcagtg cgcgcggtct cg
//
LOCUS       DASUFX010000008        20821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_973790,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000008 DASUFX010000000
VERSION     DASUFX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20821
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973790"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFV99_00265"
     CDS             complement(<1..503)
                     /locus_tag="VFV99_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX5057751.1"
                     /translation="MSESQEIRHADGRVELRAPEELTSSPLYNHDLAPVAVGKRNWNT
                     WDYAALWISMAHCIPTYTLASGMIAQGMAWWQALATILVGNLIVLAPILLNSHPGTKY
                     GIPFPVFARAAYGTTGANLPALMRALIACGWFGINTWIGGMALQVFLESLWPGWRDLL
                     GTASYPLP"
     gene            532..1512
                     /locus_tag="VFV99_00270"
     CDS             532..1512
                     /locus_tag="VFV99_00270"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5057752.1"
                     /translation="MKRAFATGGSGFVGGRLITALRARGVEVAALARSDSSAAKVETL
                     GATPVKGDLDDVAALEAGMAGCDTVFHAAAHTDQFDPVEVHMRINAKGTENVLAAARK
                     AGVKRVVHVGTEAVLADGKPIVRADESRPLPKHPMGAYPLTKGLAEQAVIAANRDGLE
                     TVVVRPRFVWGKGDTTVLPEMIDAVKRGRFGWIAGGHYLTSIVHVDNVVEGMLLAADK
                     GAPGQIYFLTDGEPVEFRDFITKLLATQGVSVGNRNVPRWLARTTVSLTSWMKKPPLT
                     KTAFALIAHEVTVDDTKARRELGYVGKKQQAEGLSEMAIASKATMTSALR"
     gene            complement(1496..3022)
                     /locus_tag="VFV99_00275"
     CDS             complement(1496..3022)
                     /locus_tag="VFV99_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057753.1"
                     /translation="MQSVGTRMLALIAIAACTPQSSQSSPPPAPAPPSTRGPLRPLAT
                     GESNIARADYVGPEACGECHAAQYAAWTTSLHRVMNARFDDPAAVIGNFKGAVLRYAG
                     GEAHFDRDLAGYTMTVRKGERETRYRITRTIGKARLQEYVGIEDGREASHEEVRLPFG
                     WWPRYGGWAPQPYFDPWLDEASFDAYAPVREPWAERCPWCHSTYPFAQRIARASGPRA
                     VGHGFEQYWASAAGTDRLVVGEQVTTGISCESCHLGGRAHAAGAAIDFLPHGATPREG
                     MPAFPTTFAAQRKDASVVNGVCAQCHSGPSPRLADGTALRNSSEALDLAASACKAKCT
                     DCHDPHKGSIDDAKSVAACSGCHATQAQPAHAGKGHETTTCLDCHMPKIVMGLDRFVR
                     THRISSPTNPQLYADAAPNACNLCHLDRPIEWTLTELRAGWDVALAPRTAAYRDNNVG
                     DVWLTSPTPAYRLIAMQAYARSPLGKYARAQIEKSLADPLAYVRAWAKLTLDTLSAAR
                     "
     gene            3171..4100
                     /locus_tag="VFV99_00280"
     CDS             3171..4100
                     /locus_tag="VFV99_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057754.1"
                     /translation="MRIAVTFLLGLTVGCGATTEDDILPPPPAGEGMQISYSAVAKAG
                     TEIWKCNVTDLPNMTYYDVNHVVSVQNESVHHMDLIAIQLAAPDLAPGEYDCADVYAN
                     YPKLMDDGILIYASQLADQQITLPTGVAAELLPKMRVMHEIHYVNTTEEDVTAYSKIN
                     AYKYPAEKVQQTIWGGAVRDTDINIPAGATSHTEWTRCTMNADVDVLFLSSHTHKLAE
                     TTVIRSFNGTDTGDVMYSNTDWHAPPLQDHTAAPIHVPQGSGFEFECHYKNAGTTDVH
                     WGFTAADEMCQIALVYTPGETTRKCEVVASGVR"
     gene            complement(4276..4665)
                     /locus_tag="VFV99_00285"
     CDS             complement(4276..4665)
                     /locus_tag="VFV99_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5057755.1"
                     /translation="MKLLLDTHVVLWSATDPERLSEGARTAIEDGANEVLVSIVSAWE
                     IALKQSIGKLDLPSHAEQWLPEVLKKSGFEVAELGLTAALRVRSLPWHHRDPFDRLLI
                     AHALEDGFTLVTHDSAFSSYGISLLAA"
     gene            complement(4662..4895)
                     /locus_tag="VFV99_00290"
     CDS             complement(4662..4895)
                     /locus_tag="VFV99_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5057756.1"
                     /translation="MRQVNIHEAKTQLSRLLEEVEAGERVVIARAGQPVAVLSPYKVA
                     VRKRRLGLFPGQARIADDFDRLPDDMAAALGAW"
     gene            complement(4931..6007)
                     /gene="ruvB"
                     /locus_tag="VFV99_00295"
     CDS             complement(4931..6007)
                     /gene="ruvB"
                     /locus_tag="VFV99_00295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5057757.1"
                     /translation="MARKKHIDVGPEDPREIGAVEKTGERAWQAALRPQSFDEYIGQR
                     DLVENLRVSVHAAKQGGWALDHFLFAGPPGLGKTTLAHVIANELGVNLHVSSGPAIDH
                     KGKLASLLTGLGERDALFIDEIHRLNPVVEENLYPAMEDFRFDLFIGEGPHAKAVTMQ
                     LPRFTLLGATTRMGLLSAPLLDRFGFHWQLRYYDLADMQAIVKRSASLIGVPIDEDGA
                     KEIARRSRGTPRIANRLLRRVRDFASVEHDGSIDGTIAASSLDRLAVDHAGLDALDRA
                     YLTVICERFDGGPVGIEAVAASLSEERGTLEEVVEPFLLQVGLIARTPRGRVATAAGF
                     QHMGLAAPSKAPSGGTGTSQGRLF"
     gene            complement(6010..6648)
                     /gene="ruvA"
                     /locus_tag="VFV99_00300"
     CDS             complement(6010..6648)
                     /gene="ruvA"
                     /locus_tag="VFV99_00300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5057758.1"
                     /translation="MIARLDGALIERMPPHIVVDVGGVGYDVVVSGYTLAGLPADGER
                     VTLRIFTHATENKLSLFGFIDQQERALFDLLITVKNVGPSTAIAILTGSNPRDIATLI
                     ANEDVPGLTRIKGVGKKTAELLVVELKDKAAEALMMWDAAGGLKSIVVPAGATRIKPL
                     GNRHPMVDEVMMALVGMGWRPAEAEQAVVDLPVPPEATIEQLLRQALRSMPR"
     gene            complement(6645..7154)
                     /gene="ruvC"
                     /locus_tag="VFV99_00305"
     CDS             complement(6645..7154)
                     /gene="ruvC"
                     /locus_tag="VFV99_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5057759.1"
                     /translation="MRILGLDPGSRVCGYGVIDEVGGELTYVECGVLTAPEERPMEQR
                     LGEIAVGLREVIGELAPAIVAVEDVFTHQNPRSALALAQARGMALAVIGLAGLRVASY
                     PPALVKKTVCGSGAADKGQIARMVQALIKLKSLPRADATDALAVAITHGRTLRPTTAK
                     IVPAVRRVS"
     gene            complement(7220..9175)
                     /locus_tag="VFV99_00310"
     CDS             complement(7220..9175)
                     /locus_tag="VFV99_00310"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX5057760.1"
                     /translation="MAGKNVALKLISSHLVDGTPKPGNEIALRIDQTLTQDATGTLVM
                     LELEAMGLDRVRTELSAQYVDHNLLQEDFKNADDHLFLRSACQRFGVWYSRPGNGVSH
                     PLHMERFGKPGKTLLGSDSHTCAAGALGMLAIGAGGLEVALAMAGEPFHVRMPAIWGI
                     RLDGQLPAWVSAKDIILEMLRRHGVAGGLGRIVEYYGPGLAQLTAMDRHVIANMGAEL
                     GATTTVFPADDRVREFLSAQDRAGDFSPLAADDAADYDAHDEIDLAALEPLVALPSSP
                     GKVVPVREVAGRDIYQAYIGSSANPGYRDFAIAAYMVDGLRVHDRVSFDINPTSRQIL
                     ENLVADGHIAPLLRAGARLHQAGCNGCIGMGQAPATDRISLRTVPRNFPGRSGTREDQ
                     VYLVSPETAAASALTGVITDPRTLAMQYPEVHEPAHPRLDLQMFVPPQPSDEARRTRL
                     ERGPNIQPLPELDPMPDAFELPVLLRLGDDISTDEILPAGARVLPLRSNIAGISEFAF
                     EPIDRSYPARARETRDTGHMIVAGSNYGQGSSREHAALAPRYLGLKIVLALRFARIHH
                     ENLVNFGVVPLTFVDPADYDRVGQGDVLTIANVRRAIRNPAPLELENKVRGLRIRAAH
                     GLSPRQVEMLLAGSLIRLMRKRLGRAA"
     gene            complement(9252..11297)
                     /locus_tag="VFV99_00315"
     CDS             complement(9252..11297)
                     /locus_tag="VFV99_00315"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5057761.1"
                     /translation="MINLAAGSALLAIAVAVVSAIAMLRADEGTARMRAVADAIREGA
                     AAFLRRELFVIAIVAAVLAIAIAAGFGGRGAELAGGFLLGALGSAAASVFGVLVSVRA
                     NVRTAAVASRATLQATLRFAFGAGSVIGLGVAGLAALELVGCYLLFGGDVPALAGLLV
                     GASLISLFARVGGGIYTKGADIGADLVGKLEIQIAEDDPRNPAVIADNVGDNVGDCGG
                     MAADLFETFLVTAVAAMLLGYLIADVRVAFPNAVLFPVAVIACGILATIAGTLVVYAT
                     RRHIEGALYGGVAATLLFAAIGLWLLCRWLMNGNTGVFVATLTGLVVVVALVLLTNYY
                     TAARFRPVARILRSTEAGAGPVVIMGMSVGLASVWLPGLVIAAGMLVAFFATGYTSTG
                     IDGQLGLYGVGLAATSMLAVMGMIISIDAFGPIVDNAGGITELAALPAETRAITDRLD
                     AAGNTTKAITKGYAIGSAALAALTLFAAFTFAAADQLAMPWSELTAQLRLDNPVVLAG
                     LLVGALLPFVFTSLLMNAVGVAARATVLEVRRQFAEIAGLAAGTARPEYGRCVSIVTS
                     TALRKLIAPGAIAVLGPLAVGLLLGPLALAGVLLGVILSGLPLALVMTTSGAAWDNAK
                     KYLERGGRTAALGAAIVGDTVGDAMKDTAGPALNPLVKVVNSVSLLCVGLIAHRGLP"
     gene            complement(11275..11778)
                     /locus_tag="VFV99_00320"
     CDS             complement(11275..11778)
                     /locus_tag="VFV99_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5057762.1"
                     /translation="MNTLQSILVPVDGSSASLSALDHAVTLARDYGARVEVFHIMPVE
                     DSLTEEARAEVQKAMDSAVQRAKRELGDRLAMIQHTGDPLVEIVRQAQQDHVDLIVMG
                     THGRIGRLHELLGGSVAEGVVRNAPCPVLTVRDKTGGYQSFADSRRYDRPPLAEQGAA
                     SDQPRSR"
     gene            complement(11867..14104)
                     /locus_tag="VFV99_00325"
     CDS             complement(11867..14104)
                     /locus_tag="VFV99_00325"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5057763.1"
                     /translation="MSPTNRWKLVSFVLAGALTYSWWHGDAHSQVRARSVQARKGPVH
                     VSAAALGVSVDDLVRQMFAAKTAEDMQPIAEKLGMVGDDAVIDALLPLVEDSREGVPS
                     VVLGVFGSIATDHAVEVLTKAAGNSRDDIHQSAIAALGATHNPRAEELLIEVAQKTSD
                     SAQTTAIDALADIASDRAIEVLAKIAGHTTDSAVAAVRSLARIEKPAAKAALLALVDS
                     PSLTVAATAIGELKDIDAAMVQKLVTIVKDGDRELVTAAIGALARAGEAGLPALREAA
                     LEGAMDVRVSAMNAMASIDNPQVLETLRSILENEDGRAADAAAAALANIDSDEAREAL
                     ISAALSEHPTTSRAVEYLMRQSGPEVEQALLVIAKSDSQERWEAVTHLVQGGNAEALA
                     LAVGQARGGADDTVKIAAMEALADSGNAAALESLIDVVRGSGDLKARAIGILGDAKPD
                     DPVVGKLLRDSVQSRDPDEAAAAAAALAKVGTPEARDALVAALGSTDGQVARNAMSSL
                     SKFRLTDDTTAAMRTALVSHPELKSQVMQQLLSGGSPYGLELAKDALTNGDAQDAYRA
                     MSALEQAASPAAFDVLMQGVRNGDAQVRAEAVATVGNMGDKRGADVLAQALRDSDAYV
                     RQTAARTLANSGTQQARELIVGMSRSQDVDDRRAAVQNLRRFEDDTTTGRLTELIRDP
                     DWNVAYTAIDTAADRPGALAALRGMISDVNIPTYQRRETARLLSYRGVNDPMIESLLS
                     SSDYE"
     gene            complement(14142..14843)
                     /locus_tag="VFV99_00330"
     CDS             complement(14142..14843)
                     /locus_tag="VFV99_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5057764.1"
                     /translation="MSTIAERWRDVRARVDAACERAGRAPSEVTIIAVSKTHPASAVR
                     EAAAAGATDFGENYAQELVAKRAECSELGGIRWHYIGRLQRNKAKLVAGQVALVHAVD
                     SVELAAELGKRAAAIQPVLLAINAAGEDTKGGINAEQAGELARSIGSIQNVRLDGLMT
                     MPPPSDDPEASRPYFEALRALRDRLVDELGIALPVLSMGMSGDFEVAIACGATHVRVG
                     TAIFGARDYKMTEQR"
     gene            complement(14840..15445)
                     /locus_tag="VFV99_00335"
     CDS             complement(14840..15445)
                     /locus_tag="VFV99_00335"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX5057765.1"
                     /translation="MLPLLLASASPRRRELLERVGVPLEVHPADVDEEPTPDEDPSAY
                     VARIAKAKAIAVKRRPGQWVLAADTTVTIDGKILAKAATPDEAAGMLGQLAGRVHQVI
                     TAFTIIGDGPHDEPLRRDGIVSSDVVVIDLDAQQIADYVASGEWRGKAGAYAVQGIGA
                     ALVREVRGSVTNVIGLPLVEVLEALRAVGAPSAKLAAGIPA"
     gene            complement(15448..16791)
                     /locus_tag="VFV99_00340"
     CDS             complement(15448..16791)
                     /locus_tag="VFV99_00340"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HEX5057766.1"
                     /translation="MKKLLLCVLFASACGGESGGDELFTPKPECMGESITPFAGTFPQ
                     VISKLAIGSVEDGFDLDKDGKPDNKLAAVSSLAQSAIDDSFKNYDILIPIEFFDFQTV
                     AKDECVKFALYLADYTTDKDNDGKKAYIDGGDCNDNVASIRTGMPEIASDGIDNDCDG
                     MADENDQNQPNTTDTADVDQDGQSVAQGDCDDHNAMIKKGAAEICHDGLDNDCDGVAD
                     RSEDASGNATACSPYDGTQDLKLDPLSLDGGAPKISFKDGTVDAANQLVAGPSIFSVQ
                     IPVYQGLNLDLRITGATIDAKLGSDATTMTGRLGGVIDAKTADTIRGLDVKDIGLTPE
                     NSLLDATFANLLGPLLALPKANADVLKKYSGCRTPDIDVDGDGLEAFCDSNPNDDNKS
                     VDVCIDGDGTVIKDGEGGTAQCSEAMKNGKPRFVDGISVEMNFQTTKVKSLKPPM"
     gene            16812..17591
                     /locus_tag="VFV99_00345"
     CDS             16812..17591
                     /locus_tag="VFV99_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057767.1"
                     /translation="MSTTQTARSMTFLNAALAVVAVTVLSGHVAPSVDDNNRYLKVTP
                     LRNGVRLAYTVFFGEIPGASERRSIDTNRDGRIDDDEANRFAAKLGTEVSAAIDIEVD
                     GAAQRIIWDLVSAGMGSDAVNAGSFSIDLVAFACGSTAGSHKILVRDQFRIPRPGETE
                     VKVEDSPGVTIQRAHVGPADDPSHDFRFAGPGGPLSDDGLELEFSATDKTPTVPPGKC
                     TASAAAATSARSGGWWRWLVIGAAFAVVLGVAFVLWRRRQT"
     gene            17570..19093
                     /locus_tag="VFV99_00350"
     CDS             17570..19093
                     /locus_tag="VFV99_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057768.1"
                     /translation="MAAAANVNLSSWRVHGAVMAALVLAVCIVTILVGERIGVNGGQG
                     WDGIGYTQWAQDFDTNVLHTGLTKYHTQRILPSATVYYALRALGVTRTVPHVISAFLV
                     LNTLLLTLGAVIWAHLGEVMKWPRAAVWAGFIALFGSFANLRHALYYPTLTDPTAFVM
                     GLAMTWAYLTKRPVVLGLVALLSTVTWPALPPIAIALLVLPRADAMIDPETRWQRVQR
                     LVALSAALALAGVFLWTARIYLLHPVPGVGDEKFAAWVRRDLLVLTVPGLIIMLGGGW
                     YLLLRQGQLWNPTGYVRQLRVRRTLIAIAAAGAIVALRAVWITKTGTHGEGPTWAQFK
                     CEHTLAAIRGPLWGIVHHVVYFGPIILVATIAWRRIAALGASWGPSAVLALALALAFA
                     AGSNSRQWNHLVPFIVAATIAVTHELWTTRRVVEFAALTLAWSKLWLVIGYDKHDNWL
                     TFPNQRYFMNQGPYANDTMYLVHLIACAVTTGLLYVLLFRSQSAVQAHGGAVGGHEP"
     gene            complement(19040..19639)
                     /locus_tag="VFV99_00355"
     CDS             complement(19040..19639)
                     /locus_tag="VFV99_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057769.1"
                     /translation="MRTQILVALLAVGCGAQTSSGDDDPMNPDDIRARILAYKAAHPG
                     SAGDINALTVDQVSADADAQWLLALCGTEQRPVIPLLAWEYGGSDHAWINPDASALVY
                     CDHTPVAANSAHWTYDAASDHVTADVAVLLADENPCASRAGAEQVSGCIGDPSNFEIL
                     VDIASYNDGVDAGLSLAEASTELRLVTADGTTVSLYSAL"
     gene            complement(19682..20404)
                     /locus_tag="VFV99_00360"
     CDS             complement(19682..20404)
                     /locus_tag="VFV99_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057770.1"
                     /translation="MLSRGVLVAIAACALAACGRFGYEERQQDASARGDTGGSGDDAD
                     TDALVTDGPTGDVPFVCPTECNGGCANNECHIDGDALTTIVCPPGIPCVITCTGFQSC
                     KADIMCGSASSCTVNCVGDHACYFGVECGAGPCTLNCSGPDACPAVSHCNNSTNCNIT
                     CSGLNSCGFTNHCGAGKCNVDCSGMDSCKGSVDCAASCQCDVTCAAIAFCPQNPGTVT
                     CPTGCSVADGCDSTLPNCAVCM"
     gene            complement(20425..>20821)
                     /locus_tag="VFV99_00365"
     CDS             complement(20425..>20821)
                     /locus_tag="VFV99_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5057771.1"
                     /translation="ALGGIPRDEVYVTNAVKHFKYTQRGKRRLHDKPTRYEIAACKPW
                     LRSELEVVEPDIVVLLGATAAQALLGASFRVTKERGHVIEGTGIARWTFATVHPASVL
                     RAPDDELRRLAKEDFIHDFELVGDYFKKL"
BASE COUNT         3466 a         7077 c         6766 g         3512 t
ORIGIN      
        1 ggcagcggat agctcgcggt gccgagcagg tcgcgccaac cgggccacag cgactcgagg
       61 aacacctgca gcgccatgcc gccgatccag gtgttgatgc caaaccaacc gcaggcgatc
      121 agcgcgcgca tcagcgccgg caggttcgcg ccggtcgtgc cgtaggcagc gcgcgcgaac
      181 accgggaacg ggatgccgta cttggtaccg ggatgcgagt tgagcaggat cggcgcgagc
      241 acgatcaggt tgccgacgag gatcgtggcg agcgcctgcc accacgccat cccctgcgcg
      301 atcatgccgg acgcgagcgt gtacgtcgga atgcagtggg ccatcgagat ccacagcgcg
      361 gcgtagtccc acgtgttcca gttgcgcttt ccgaccgcga caggtgcgag gtcgtggttg
      421 tagagcggcg acgacgtcag ctcctcgggc gcgcgcaact cgacacgacc atccgcgtga
      481 cgaatctcct ggctctcgct catagcgctc gaacctagta aattccgccg cgtgaagcga
      541 gcgttcgcta caggtggctc tggtttcgtc gggggtaggc tcatcacggc gctgcgcgcg
      601 cggggtgtgg aggtcgcggc gctggcccgc agtgacagct cggccgccaa ggtcgagacg
      661 ctcggcgcga cgccggtgaa gggcgacctc gacgacgtcg cggcactcga agccggaatg
      721 gccggttgcg acaccgtgtt tcatgcggcc gcgcacaccg atcagttcga cccggtcgag
      781 gtccacatgc ggatcaatgc gaagggcacc gagaacgtgc tcgcggcagc gcgcaaagcg
      841 ggcgtcaagc gtgtggtcca cgtcggaacc gaagccgtgc tcgccgatgg caagccgatc
      901 gtgcgcgccg acgagagtcg cccgctgccg aagcacccga tgggcgccta tccactcacg
      961 aagggccttg ccgagcaggc cgtgatcgcc gcgaaccgcg atggtctcga aaccgtcgtg
     1021 gtgcggccgc gcttcgtgtg gggcaagggt gacacgaccg tattgcccga gatgatcgac
     1081 gcggtgaagc gcggccggtt tggctggatc gccggcggtc attacctgac gtcgatcgtg
     1141 cacgtcgaca acgtggtcga agggatgttg ctcgcggccg acaagggcgc gcccggccag
     1201 atctacttcc tcaccgatgg cgagccggtc gagttccgcg atttcatcac gaagctgctc
     1261 gcgacccagg gtgtctcggt cggcaaccgc aacgtgccgc gctggctcgc gcgcaccacg
     1321 gtgtcgctga cgtcgtggat gaagaagcca ccgctgacga agaccgcgtt cgcgctgatc
     1381 gcgcacgagg tcactgtcga cgacaccaag gcgcgccgcg agctcggcta cgtcggcaag
     1441 aagcagcaag ccgaaggact ctcggagatg gcgatcgcgt cgaaggcgac gatgactagc
     1501 gcgctgcgct gagcgtatcg agcgtcagct tcgcccacgc tcgcacgtac gcgagcggat
     1561 cggcgaggct cttctcgatc tgcgcgcgcg cgtatttgcc gagcggcgag cgcgcgtatg
     1621 cctgcatcgc gatcaaccgg tacgctggcg tcgggctggt gagccagaca tcaccgacgt
     1681 tgttgtcgcg ataggcggcg gtgcgcggcg cgagcgcgac gtcccagccc gcacgcagct
     1741 cggtgagcgt ccactcgatc gggcgatcga ggtgacacag gttgcacgcg ttcggcgccg
     1801 cgtcggcgta gagctgcggg ttcgtcggcg agctgatccg gtgcgtgcgc acgaagcgat
     1861 cgaggcccat cacgatcttc ggcatgtggc aatcgaggca cgtcgtcgtc tcgtgaccct
     1921 taccggcgtg cgcgggctgc gcttgcgtgg cgtgacagcc ggagcatgca gcgaccgact
     1981 tcgcatcgtc gatgctgccc ttgtgtggat cgtgacagtc ggtgcacttc gctttgcacg
     2041 ccgatgcggc gagatcgagc gcctcgctcg agttgcgcaa ggcggtgcca tcggcgagcc
     2101 gcggcgatgg ccccgagtga cattgcgcgc acacgccgtt gaccacgctc gcgtccttgc
     2161 gttgcgccgc aaacgtcgtc gggaatgcgg gcatgccctc gcgcggcgtc gccccgtgcg
     2221 ggagaaagtc gatcgcggcg ccggccgcgt gcgcgcgccc accgaggtgg cagctctcgc
     2281 agctgatccc ggtcgtcacc tgctcgccga cgacgaggcg atcagttccc gccgccgacg
     2341 cccagtactg ctcgaagccg tggccgaccg cgcgcgggcc cgacgcacgc gcgatgcgct
     2401 gcgcgaatgg atacgtcgaa tgacaccacg gacaccgctc ggcccatggc tcgcgcacgg
     2461 gcgcgtaggc gtcgaagctc gcctcgtcga gccacgggtc gaaatacggc tgcggtgccc
     2521 agccgccgta gcggggccac cacccgaacg gcagccggac ttcttcatgg ctcgcttcgc
     2581 ggccgtcctc gatgccgacg tactcctgca gccgcgcctt gccgatcgtg cgcgtgatcc
     2641 gatagcgagt ctcgcgctcg cctttgcgca cggtcatcgt gtagccggcg agatcgcgat
     2701 cgaagtgcgc ctcgccgccc gcgtagcgca gcacggcgcc cttgaagttc ccgatcacgg
     2761 cggctggatc atcgaaccgc gcgttcatca cgcggtgcag gctcgttgtc cacgccgcgt
     2821 attgagcagc gtggcattca ccgcacgctt cgggaccgac gtagtccgcg cgcgcgatgt
     2881 tgctctcgcc ggtcgcgagt ggacgcagcg ggccacgcgt tgacggcgga gccggggccg
     2941 gcggcggcga cgactgcgac gactgcggcg tgcacgcagc aatcgcgatc agcgcgagca
     3001 tgcgtgtgcc gaccgattgc accgctctag gctgcgccga atccgcctta gtggctagtc
     3061 cagacgccag cgtcgtcgca cttcgcctca ccgcgccttg ttgactccga gtcaagtacg
     3121 cggatccggc gccggtgaca cgtggcatcg gcattgctcg caggtgccgc atgcgcattg
     3181 cggtgacgtt cttgctcggc ctgacagtcg gatgtggtgc aacaaccgaa gatgacattc
     3241 tgccgccgcc tccggccggc gaaggaatgc agatcagcta cagcgcggtc gcaaaggcgg
     3301 gcaccgagat ctggaagtgc aacgtcaccg acctgccgaa catgacctac tacgacgtga
     3361 atcacgtcgt gtcggtccag aacgagtcgg tccaccacat ggacctgatc gcgatccagc
     3421 tcgccgcgcc cgacctcgcg ccgggcgagt acgactgcgc cgacgtctac gcgaattacc
     3481 cgaagctgat ggacgacggg atcctgattt acgcgagcca actcgccgat cagcagatca
     3541 cgctgccgac cggcgtcgcc gccgagctac tgccgaagat gcgcgtgatg cacgagatcc
     3601 actacgtcaa cacgacggag gaggacgtca ccgcgtactc gaagatcaac gcgtacaagt
     3661 atcccgccga gaaggtccag cagacgatct ggggcggcgc ggttcgcgac accgatatca
     3721 acatcccggc cggcgcgacg tcgcataccg agtggacgcg ctgcacgatg aacgcggacg
     3781 tcgacgtgct gttcctgtcg tcgcacacgc acaagctcgc cgagacgacc gtgatccgca
     3841 gcttcaacgg cactgatacc ggtgacgtca tgtactcgaa caccgattgg cacgcgccgc
     3901 cgctgcaaga tcacaccgct gcgccgatcc acgtgccgca gggttcgggc ttcgagttcg
     3961 agtgccacta caagaacgcc gggacgaccg atgtccactg gggcttcacg gctgcggacg
     4021 agatgtgcca gatcgcgctc gtctacacgc cgggcgagac cacgcgcaag tgcgaggtcg
     4081 tcgcgtcggg tgtccggtag tcgtgccgga cgcgcgctgc cggacagccg ccgcgatgag
     4141 agcggctcga agctctgcga agatgcagtc acgtttcgtc gcgaacgcta cgttcccata
     4201 cgaacatcat cttgctctgg aatgcgaagt cggtcgcggc cgtcgcggcg ggcgtgcgct
     4261 aatcatgaag ccgcgctacg ccgctagcag cgaaatgccg tacgacgaaa atgcgctgtc
     4321 gtgcgtgacg agtgtaaagc cgtcttcgag ggcatgagcg atcaacaacc gatcgaaagg
     4381 gtctcggtga tgccagggca gcgaccgcac gcgaagtgcg gcggtgagtc cgagctccgc
     4441 gacttcgaat ccgctcttct tcagtacctc gggcaaccat tgttcggcat ggctcggaag
     4501 gtcgagtttc ccgatcgatt gcttgagtgc gatctcccat gcactgacga tgctgacgag
     4561 gacctcgttc gcgccatcct cgatcgcggt gcgagccccc tccgacaatc gctccggatc
     4621 cgtcgcactc cacagcacga catgcgtgtc gagaagaagt ttcaccaagc tcccaatgcc
     4681 gcggccatat cgtcgggaag gcgatcgaag tcgtcggcga tgcgcgcctg tccggggaac
     4741 agacccagcc ggcgcttgcg cacggcgact ttgtacggtg aaaggaccgc caccggctga
     4801 cccgcacgtg cgatcaccac gcgttcaccg gcctcgactt cttcgagtag ccgagacagc
     4861 tgggtcttcg cctcgtgaat gttcacctga cgcatgtctc tagcttagct aaccatgact
     4921 aattgacaag tcagaacaac cgaccttgac tcgtgccggt gccgccgctc ggtgccttgc
     4981 tcggcgcggc gaggcccatg tgctggaaac ctgccgccgt cgcgacgcgg ccgcgtggcg
     5041 tgcgcgcgat gaggccgacc tggagcagga acggctcgac gacctcttcg agcgtgccgc
     5101 gctcctcgga cagcgacgcc gcgactgcct cgatgccgac cggaccgccg tcgaaccgct
     5161 cgcagatgac ggtgaggtac gcgcgatcga gcgcatcgag accagcgtga tcgaccgcga
     5221 ggcgatcgag cgacgacgcg gcgatggtgc cgtcgatcga gccatcgtgc tcgaccgacg
     5281 cgaagtcgcg gacccggcgc agcaggcggt tcgcgatgcg cggcgtaccg cgcgagcgcc
     5341 gcgcgatttc cttcgcgcca tcctcgtcga tcggcacccc gatcagcgac gccgagcgtt
     5401 tcacgatcgc ctgcatgtcg gcgaggtcgt agtagcgcag ctgccagtgg aacccgaagc
     5461 gatcgagcag cggcgccgag agcaggccca tgcgcgtcgt cgcgccaagc agcgtgaagc
     5521 gcggcagctg catcgtgacg gccttcgcgt gcgggccttc gccgatgaac aggtcgaagc
     5581 ggaagtcctc catcgcggga tagaggttct cctcgacgac ggggttcagc cgatggatct
     5641 cgtcgatgaa gagggcgtcg cgctcgccga gaccggtgag cagcgatgcg agcttgccct
     5701 tgtgatcgat cgcggggccg gagctgacgt gcaggttgac gccgagctcg ttcgcgatca
     5761 cgtgcgcgag cgtggtcttg ccgaggccgg gcggaccggc gaacaggaag tgatcgagcg
     5821 cccagccgcc ttgcttcgcg gcgtgcaccg agacccgtag gttctcgaca aggtcgcgct
     5881 ggccgatgta ctcgtcgaag ctctgcgggc gcagggccgc ttgccacgcg cgctcgccgg
     5941 ttttctcgac cgcgccgatc tcgcgcggat cttcgggccc gacgtcgatg tgcttcttgc
     6001 gcgccatggt tacctcggca tgctccgcag cgcctgtcgc agcagctgct cgatcgtggc
     6061 ttcaggcggc accggcaggt cgacgaccgc ctgctccgcc tcggcggggc gccaacccat
     6121 gccgaccagc gccatcatca cttcatcgac catcgggtgc cggttgccca gcggcttgat
     6181 gcgcgtcgcg cccgccggca cgacgatcga cttcagacca cccgccgcgt cccacatcat
     6241 cagcgcctcg gcggccttgt ccttgagctc gacgacgagc aactcggccg tcttcttgcc
     6301 gacgcccttg atgcgcgtga gcccgggcac gtcctcgttt gcgatcagcg tcgcgatgtc
     6361 gcgcgggttc gagccggtca agatcgcgat cgccgtcgac ggcccgacgt tcttcaccgt
     6421 gatcaacaga tcgaacagcg cgcgctcttg ctgatcgatg aagccgaaca acgagagctt
     6481 gttctcggtc gcgtgcgtga agatgcgcag cgtgacgcgc tcgccatcgg cgggcaatcc
     6541 ggcgagcgtg tagccggaca ccacgacgtc gtagccaaca ccgccgacgt cgaccacgat
     6601 gtgcggcggc atgcgttcga tcagagctcc atcgagtcga gcaatcacga cactctcctg
     6661 acggccggaa caatcttcgc cgtggtcggg cgcaacgtgc ggccatgcgt gatcgcgacg
     6721 gcgagcgcat cggtcgcgtc ggcgcgcggc aagctcttca gcttgatcag cgcttgcacc
     6781 atgcgcgcga tctggccctt gtcggcagcg cccgagccac agaccgtctt cttgacgagc
     6841 gcgggcggat acgacgcgac gcgaaggccg gcgagcccga tcaccgcgag cgccatgccg
     6901 cgtgcttgcg cgagtgcgag cgcgctgcgc gggttctgat gcgtgaacac gtcttcgact
     6961 gcgacgatcg caggcgcgag ctcaccgatc acctcgcgca ggccgaccgc gatctcgccg
     7021 agccgctgct ccatcgggcg ttcttcgggc gcggtcagca cgccgcactc gacatacgtg
     7081 agctcgccgc cgacctcgtc gatgacgcca tagccgcaca cgcggctgcc ggggtcgagc
     7141 ccgaggattc gcacgcccca ccctactcta ggcctccgac atcacgccaa tatcgagccg
     7201 gcgcagggag atcgccttgt taggcggcgc ggcccaggcg tttgcgcatg agccggatca
     7261 ggctacccgc gagcagcatc tcgacctggc gtggcgacaa cccatgtgcc gcgcgtatac
     7321 gcaggccacg caccttgttt tccagctcga gaggggccgg attgcggatc gcgcggcgaa
     7381 cgttcgcgat ggtcaagaca tcgccttgcc cgacgcggtc gtagtcagcg ggatcgacga
     7441 acgtgagcgg cacgacgccg aagttgacga ggttctcgtg gtggatacgc gcaaaccgca
     7501 gtgcgagcac gatcttgagg ccgaggtaac gtggcgcgag tgcggcgtgc tcgcggctcg
     7561 aaccctggcc gtagttactg ccggcgacga tcatatgacc ggtgtcccgg gtctcgcgtg
     7621 cgcgtgctgg gtacgagcga tcgatgggtt cgaacgcgaa ctcactgatc ccggcgatgt
     7681 tgctgcgcag cgggagcacg cgcgcgccgg ccggcaggat ctcgtcggtc gaaatatcgt
     7741 cgccgagcct gagtaacacc ggcagctcga aagcgtcggg catcggatcg agctcgggca
     7801 gcggctggat attcggtccg cgttcgagcc gtgtgcggcg cgcctcgtcg gacggttgag
     7861 gcggcacgaa catctggaga tcgagccgcg gatgtgcggg ctcgtgaacc tccggatact
     7921 gcatcgcaag cgtgcgcggg tccgtgatca cgccggtcag tgccgacgcc gctgcagtct
     7981 cggggctcac gagatagacc tgatcctcgc gcgtgcccga acgcccggga aaattgcgtg
     8041 gcacggtgcg caggctgatc cgatccgtcg cgggtgcttg tcccatcccg atacagccgt
     8101 tgcatcctgc ctggtgaagc cgcgcgcccg cacgcaagag cggcgcgatg tgaccatcgg
     8161 cgacgaggtt ctcgaggatc tggcgcgagg tcgggttgat gtcgaacgac acgcgatcgt
     8221 ggacgcgcag cccgtcgacc atgtacgcgg cgatcgcgaa gtcgcgatag cccggatttg
     8281 ccgacgaacc gatgtacgcc tgataaatgt cgcgacctgc cacttcgcgc accggcacca
     8341 ccttgcccgg gctcgacggc agcgcgacga gcggctcgag cgccgcaagg tcgatctcat
     8401 cgtgggcgtc gtagtcggca gcatcgtccg cggcgagcgg cgagaagtcg cccgcgcgat
     8461 cctgagccga caggaactcg cgcacgcggt cgtcggccgg aaacaccgtc gtcgtcgcgc
     8521 cgagctcagc gcccatgttc gcgatcacgt ggcggtccat cgccgtcagc tgcgcgagcc
     8581 cggggccgta gtattcgacg atccggccga gaccgcccgc cacgccgtga cgtcgcagca
     8641 tctcgaggat gatgtccttc gcgctcaccc acgccggcag ctggccgtcg agccggatgc
     8701 cccagatcgc gggcatgcgc acgtggaacg gttcgcctgc catcgcgagc gcgacctcga
     8761 gcccgccggc accgatcgcg agcatcccga gtgcgcccgc ggcacatgtg tggctatccg
     8821 acccgagcag cgtcttgccc ggtttgccga accgctccat gtgcagcgga tggctgacgc
     8881 cgttaccggg ccggctgtac cagacaccaa accgctgaca cgcgctgcgc aggaacaggt
     8941 gatcgtctgc gttcttaaag tcttcttgca gcagattgtg gtcgacgtat tgcgctgaca
     9001 gctcggtacg cacgcggtcg aggcccatcg cttcgagctc gagcatcacg agcgtgccgg
     9061 tcgcatcctg cgtcaacgtc tgatcgatgc gcagcgcgat ttcgttgcca ggtttcgggg
     9121 ttccatcgac gaggtgcgag gagatcagct tcagcgcgac gttcttacca gccatacaca
     9181 aacatgcttg caatgcgtgt gctctgcctg ctgtgcggtg gtgaaccggt ggcctaccgg
     9241 tcgacaccag gtcacggcag ccctcggtgc gcgatcaatc cgacgcacaa cagcgacacg
     9301 gaattgacca ccttgacgag cggattcagc gctgggcccg cggtgtcctt catcgcgtcg
     9361 ccgacggtat cgccaacgat cgcggcaccg agcgccgccg tgcgccctcc tcgttcgagg
     9421 tacttcttcg cgttgtccca cgcggcgcca ctcgtggtca tcacgagcgc gagcggcagg
     9481 ccggagagga tcacgccgag cagcacgccg gcgagcgcga gcggcccgag caacagtccg
     9541 acggcgagcg gccccaggac tgcgatcgca ccgggcgcga tcaacttgcg aagtgcggtc
     9601 gaggtcacga tcgagacgca cctgccgtat tcgggacgtg ctgtgccggc ggcgagccct
     9661 gcgatctcgg cgaactgtcg ccgcacctcg aggacggtcg cccgcgccgc cacaccgacg
     9721 gcgttcatca gcagcgacgt gaacacgaac ggcaagagcg cgccgacaag caaaccggcg
     9781 aggacgaccg gattgtccag cctgagctgg gcggtgagct cggaccacgg catggcgagc
     9841 tgatcggccg cagcgaacgt gaacgccgcg aacaacgtga gcgcggcaag cgctgccgaa
     9901 ccgatcgcgt agcccttggt gatcgccttc gttgtattcc ccgcggcgtc gagccggtcg
     9961 gtgattgcac gcgtttcggc tggcagcgcg gcaagctcgg tgatgcctcc cgcgttatcg
    10021 acgatcggtc caaacgcgtc gatcgagatg atcatgccca tcacggcgag catgctcgtc
    10081 gccgcaagac ccaccccgta gagcccgagt tggccatcga tcccggtgct cgtgtacccg
    10141 gtcgcgaaga acgcgacaag catgccggct gcgatcacga gaccgggcaa ccagacggac
    10201 gcgagaccga ccgacatacc catgatcacg accggtcccg cgccggcttc ggtcgagcga
    10261 aggattcgcg ccaccggacg aaaccgtgcc gcggtgtagt agttggtcaa cagaaccagc
    10321 gcgacgacga cgaccagccc ggtcaacgtc gcaacgaaca cgccggtgtt gccgttcatc
    10381 agccaacggc acaagagcca cagtccgatc gcggcgaaca gcagcgttgc ggcaacgccg
    10441 ccgtatagcg ccccttcgat gtggcgacgc gtcgcgtaga cgacgagcgt acctgcgatc
    10501 gtcgcgagaa tgccgcacgc gataaccgcg acgggaaaca gcaccgcgtt gggaaacgcg
    10561 acccgaacat ccgcgatcag atagccgagc agcatcgccg caacggcggt gaccaagaac
    10621 gtctcgaaca ggtcagccgc catgccgccg cagtcaccga cgttgtctcc cacgttgtct
    10681 gcaatcaccg ctgggttgcg tggatcgtcc tccgcgatct gaatctcgag cttgccgacg
    10741 aggtccgcac cgatgtccgc tcccttcgtg tagatgccgc cgccgacgcg cgcgaacaag
    10801 ctgatcagcg aggcaccgac gagaagcccg gccaacgccg gcacatcacc accgaacagg
    10861 aggtaacacc cgaccagctc gagcgccgcg aggcccgcca cgccgaggcc gatgacagag
    10921 ccggcaccga acgcgaagcg caacgttgcc tgcagcgtcg cgcgcgacgc gactgccgcc
    10981 gtccgcacgt tcgctcgcac cgacacgagg acgccgaaca cgctcgccgc agcgcttccg
    11041 agtgcaccga gaaggaagcc gccggcaagc tcggcccctc gcccaccaaa cccggcggcg
    11101 atcgcgatcg cgagcaccgc ggcaacgatc gcgatcacga acagctcgcg ccgcagaaac
    11161 gctgcggctc cttcgcggat cgcatctgcg acggcacgca tgcgcgcggt gccttcatcc
    11221 gcgcggagca tcgcgatcgc actgacgacg gcgaccgcaa tcgcgagcag cgcgctaccg
    11281 gctgcgaggt tgatcactgg ccgcaccctg ctcggcgagc ggcggccggt cgtaacgccg
    11341 gctatcggcg aacgattggt acccgccggt cttgtcgcgc acggtcaaca ccggacatgg
    11401 agcgttgcgg acgacgcctt cggcaacact gccgccgagc aactcgtgca agcgtccgat
    11461 tcgtccgtgc gttcccatga cgatcaagtc gacatgatct tgttgcgctt gccgcacgat
    11521 ctcgaccagt ggatcgccgg tgtgctggat catcgcgaga cgatcgccaa gctcacgctt
    11581 cgcacgttga accgcgctgt ccatcgcttt ctggacctcc gcccgcgcct cctcggtgag
    11641 cgaatcctcg accggcatga tgtggaagac ctcgactcgt gcgccgtaat cacgcgcgag
    11701 agtgactgca tggtcgaggg cggatagcga agcggacgat ccatcgaccg gcaccaggat
    11761 ggactgcaag gtgttcatgc cccgttaccg tgcaacgcgc attccacgat gctcgatcgg
    11821 cgctatcgtc ggccctgcgc cgatcatcga ttcctggatc gacgacttac tcgtagtcgc
    11881 tcgaggacaa gagcgactcg atcatcgggt cgttgacgcc gcggtagctg agcaggcgtg
    11941 ccgtctcgcg ccgctggtag gtcgggatat tgacgtcgct aatcattccg cgcagtgcgg
    12001 caagcgcgcc gggacggtcc gccgccgtgt cgatcgcggt gtacgcgacg ttccagtcgg
    12061 gatcgcgaat cagctcggtc agccgcccgg tggtcgtgtc gtcctcgaac cggcgcaggt
    12121 tctgaaccgc cgcgcggcga tcgtcgacat cctggctgcg cgacatgccg acgatcagct
    12181 cgcgcgcctg ctgcgtgcca ctgttggcaa gcgtgcgcgc ggcggtttgg cgcacgtagg
    12241 catcggagtc gcggagcgcc tgcgcgagca cgtcggcgcc gcgcttgtcg cccatgttgc
    12301 cgaccgtcgc gaccgcttcg gcgcgcacct gcgcatcgcc attgcgcacg ccctgcatca
    12361 gcacgtcgaa cgcggcgggc gatgccgctt gctcgagcgc cgacatcgcg cggtaggcat
    12421 cctgcgcgtc gccgttcgtg agcgcatcct tcgccagctc gaggccatac ggtgagccac
    12481 ccgagaggag ctgctgcatc acttggctct tgagctcggg atgcgaaacg agcgccgtgc
    12541 gcatcgcggc tgtcgtgtca tcggtgagcc ggaacttcga cagcgacgac atcgcgttgc
    12601 gcgcgacttg cccgtccgtg ctgccgagcg ccgcgaccaa cgcatcgcgt gcttccggcg
    12661 tgccgacctt cgcgagcgcc gccgcagcag ccgctgcctc gtcgggatca cgcgactgca
    12721 ccgaatcgcg gagcagcttg ccgaccaccg gatcgtcggg cttcgcatcg ccgaggatgc
    12781 cgatcgcgcg cgccttgaga tcgccgctgc cgcgcacgac atcgatcaac gattcgagag
    12841 ccgccgcgtt gcccgagtcg gccagcgcct ccatcgccgc gatcttcacg gtgtcgtcag
    12901 caccgccgcg cgcttgaccg accgcaagcg cgagcgcttc ggcattgccc ccttggacca
    12961 ggtgcgtgac cgcctcccag cgctcctggc tgtcggactt cgcgatcacg agcaacgcct
    13021 gctcgacctc ggggccgctc tggcgcatca ggtactcgac cgcgcgcgac gtcgtcggat
    13081 gctccgacag cgcagcggag atcagcgcct cgcgcgcttc gtcgctatcg atgttcgcga
    13141 gtgccgcggc cgcagcatcg gcggcgcgtc cgtcctcgtt ctcgaggatc gagcgcagcg
    13201 tctcgagcac ctgtgggttg tcgatgctag ccatcgcgtt catcgctgac acgcgcacgt
    13261 ccatcgcgcc ttcgagcgca gcctcgcgca acgccggcag acccgcctcg cccgcgcgcg
    13321 cgagcgcacc gatcgcggcg gtcacgagct cgcggtcgcc atccttcacg atggtgacga
    13381 gcttttgaac cattgcagcg tcgatatcct tgagctcgcc gattgccgtt gccgccaccg
    13441 tgaggctcgg cgaatcgacg agcgccagca gcgctgcctt cgcggccggc ttctcgatcc
    13501 tcgcgagcga gcgcaccgcg gcgactgcgc tatcggtcgt gtggccggcg atcttcgcga
    13561 gcacttcgat cgctcgatcg cttgcgatgt cggcaagcgc gtcgatcgcg gtcgtctgcg
    13621 cgctatcgct cgtcttctgc gcgacctcga tcagcagctc ctccgcacgc gggttgtgcg
    13681 tggcgccgag tgccgcgatc gcggactgat gaatatcgtc gcgcgaattg ccggccgcct
    13741 tggtcagtac ctcgacggcg tgatcggtcg cgatcgaacc gaagacgccg agcaccaccg
    13801 acggcacacc ctcgcgcgaa tcctcgacga gcggcagcag tgcatcgatc accgcatcgt
    13861 cgcccaccat gccgagcttc tcggcgatcg gctgcatgtc ctcggccgtc ttcgccgcga
    13921 acatctggcg caccaggtcg tcgaccgaca cgccgagcgc ggctgcactg acgtgtacgg
    13981 gcccctttcg cgcctgcacc gagcgcgcgc gcacctggct gtgcgcgtcg ccgtgccacc
    14041 acgaatacgt gagcgcacct gcgagcacga agctgaccag cttccagcga ttcgtcggac
    14101 tcatcccgcg agtagagtga caacgcaccc cgctgcgggt ttcatctctg ttctgtcatc
    14161 ttgtaatcac gtgcgccgaa gatggctgtc ccaactcgca catgtgtcgc accacatgcg
    14221 atggccactt cgaagtcgcc actcatcccc atcgacaaca caggcaacgc gatgccgagc
    14281 tcgtcgacga gacgatcgcg aagcgcgcgc aatgcctcga agtacggccg gctcgcctcg
    14341 ggatcgtcgg agggcggcgg catcgtcatc aggccgtcga gccggacgtt ctggatcgat
    14401 ccgatcgaac gcgcgagctc gccggcttgt tccgcgttga tgccaccttt cgtgtcctcg
    14461 ccggcggcgt tgatcgcgag caataccggt tggatggcag cggcgcgctt gccgagctcg
    14521 gccgcgagct cgacgctatc gaccgcatga accagcgcga cctgaccggc gacgagcttt
    14581 gccttgttgc gctggagccg gccgatgtag tgccagcgga tgccacccaa ttcgctgcat
    14641 tctgcgcgct tcgcgacgag ctcctgcgcg tagttctcgc cgaagtccgt cgcacctgcg
    14701 gccgcagcct cgcgcacggc ggacgcagga tgcgtcttgc tcaccgcgat gatcgtgacc
    14761 tcgctcggtg cacgtcccgc tcgttcgcac gcggcatcga cacgcgcgcg cacatcgcgc
    14821 cagcgctcgg cgatcgtgct catgccggaa tcccggccgc gagcttcgca ctcggcgcac
    14881 cgaccgcacg cagcgcctcg agcacctcga cgagcggcaa gccgatcacg ttcgtcaccg
    14941 agcctcgcac ttcgcgcacg agcgccgcac cgatgccttg gaccgcatat gcaccggcct
    15001 taccacgcca ctcgccgctc gccacgtagt cggcgatctg ctgggcatcg agatcgatga
    15061 cgacgacatc gctcgagacg atgccgtcgc gccgcagcgg ctcgtcgtgc ggaccgtcgc
    15121 cgatgatcgt gaacgcggtg atcacctgat ggacacgccc ggcgagctgg cccagcatcc
    15181 ccgcggcttc gtccggcgtc gccgccttcg cgaggatctt gccatcgatc gtgacggtcg
    15241 tgtcggccgc gagcacccat tgcccggggc gccgcttcac cgcaatcgcc ttggctttcg
    15301 cgatccgcgc gacatacgcg gacggatcct cgtcgggtgt cggctcctcg tcgacatccg
    15361 caggatgcac ctcgagcggt acgccgacgc gctcgagtag ctctcgacgg cgtggtgacg
    15421 ccgacgccag cagcaggggc agcattgcta catcggcggc ttgagcgact tcaccttggt
    15481 cgtctggaag ttcatctcga ccgagatgcc gtcgacgaag cggggcttgc cgttcttcat
    15541 cgcttcgctg cactgagcgg tgccgccctc gccatccttg atgacggtgc cgtcgccgtc
    15601 gatgcagacg tcgaccgact tgttgtcgtc gttcgggttc gagtcgcaga acgcctcgag
    15661 accgtcgcca tcgacgtcga tatcaggcgt gcggcagccc gagtacttct tgagcacgtc
    15721 cgcattcgcc ttcggaagcg cgagcagcgg gccgaggagg ttcgcgaacg tcgcgtcgag
    15781 cagcgagttc tccggggtca ggccgatatc tttaacgtcg aggccacgga tcgtgtcagc
    15841 ggtcttcgcg tcgatgacgc cgccgaggcg gcccgtcatc gtcgtggcgt cagagccgag
    15901 ctttgcgtcg atggtcgccc cggtaatacg caggtccaag ttgagacctt gatacactgg
    15961 aatctgcacg ctgaagatcg aagggccagc caccaactgg ttggccgcgt cgaccgtgcc
    16021 gtccttgaag gagatcttgg gcgcgccgcc gtccaacgac aaggggtcga gcttgaggtc
    16081 ctgcgtgccg tcgtacgggc tgcacgctgt cgcgttgccc gaggcgtcct cgctacggtc
    16141 ggcgacgccg tcgcagtcgt tgtcgagacc gtcgtggcaa atctcggcgg cgcccttctt
    16201 gatcatcgcg ttgtgatcgt cgcagtcgcc ctgcgcgacg gattggccat cctgatcgac
    16261 gtccgccgtg tcggtggtgt tcggctggtt ctgatcgttt tcgtcggcca tgccatcgca
    16321 gtcgttgtcg atgccgtcac tggcgatctc cggcatgcct gtgcgaatcg acgcgacgtt
    16381 gtcgttgcag tcgcctccgt cgatgtacgc cttcttgccg tcgttgtctt tgtcggtcgt
    16441 gtagtcggcg aggtagagag cgaacttcac gcactcgtcc ttcgcgacgg tctgaaagtc
    16501 gaagaactcg atcgggatca ggatgtcgta gttcttgaac gagtcatcga tcgcgctctg
    16561 cgcgagcgac gacaccgcgg cgagcttgtt gtccggcttg ccgtccttat cgagatcgaa
    16621 gccgtcctcg acgctaccga tcgcgagctt ggagatgacc tgagggaacg tccccgcaaa
    16681 aggcgtaatc gattcgccca tgcactcggg ctttggcgtg aacagctcgt cgccacccga
    16741 ttccccgcca cacgcgctcg cgaacagaac acaaagcaat agcttcttca tggggtaccc
    16801 tcgggccgga cttgagcacg acgcaaacag cccggtcaat gacctttctc aacgccgcac
    16861 tcgccgtggt cgccgtcacg gtcctctcgg gtcacgtggc cccatcggtc gatgacaaca
    16921 accggtactt gaaggtcacg ccgctgcgca acggcgtccg attggcctat acggtgttct
    16981 tcggcgagat ccccggggct tccgagcgcc ggtctatcga tacgaaccgc gacggccgca
    17041 tcgacgacga tgaggccaac cggttcgcgg ccaagctggg gacagaagtc tccgccgcca
    17101 tcgatatcga agtggatggg gcggcacaac gaataatttg ggacttggtg tccgccggca
    17161 tgggcagcga cgcggtgaat gccggctcgt tctcgatcga cctcgtcgcg ttcgcctgcg
    17221 gcagcaccgc cggcagccac aagattctcg ttcgcgacca gttccgcatt ccccgccctg
    17281 gcgagaccga ggtcaaggtg gaggacagcc ccggcgtcac gatccagcgc gcgcacgtcg
    17341 gcccggccga cgatccaagc catgacttcc gcttcgccgg acccggcggt ccgctctccg
    17401 acgacggcct cgagctcgag ttctccgcca ccgacaagac cccgaccgtg ccgcccggca
    17461 aatgcacggc ctccgcggcg gccgcgacgt ccgctcgcag cggtggctgg tggcgctggc
    17521 tcgtcatcgg cgcggcattc gcggtcgtgc tcggcgtagc gttcgtgttg tggcggcggc
    17581 ggcaaacgtg aacctgtcgt cctggcgcgt ccacggcgcc gtgatggcgg cgctcgtgct
    17641 cgcggtctgc atcgtgacga tcctcgttgg cgagcgcatc ggcgtgaacg gcggccaggg
    17701 ctgggacggc atcggctaca cgcaatgggc gcaggacttc gacaccaacg tcctccacac
    17761 cgggctcacg aagtaccaca cgcagcggat cctgccgtca gctaccgtgt actacgcact
    17821 gcgcgcgctc ggcgtcacgc gcaccgtgcc gcacgtgatc agcgcattcc tcgtgctcaa
    17881 cacactgctg ctcacgctcg gcgcggtgat ctgggcacat ctcggcgaag tcatgaagtg
    17941 gccgcgcgcg gcagtgtggg ccggtttcat cgcgctgttt ggttcattcg cgaacctgcg
    18001 ccacgcgctc tactacccga cgctcactga tccgaccgcg ttcgtgatgg gcctcgcaat
    18061 gacgtgggcg tatctgacca agcgcccggt cgtgctgggg ctcgtcgcgc tgctctcgac
    18121 cgtgacatgg cccgcgctgc cgccgatcgc gatcgcgcta ctcgtactgc cacgcgccga
    18181 cgcgatgatc gatcccgaaa cgcgctggca gcgcgtacag cggctcgtcg cgctcagcgc
    18241 cgcgctcgcg ctggccggcg tgtttctgtg gaccgcacgc atatacctgc tccatccggt
    18301 gcccggcgtc ggcgacgaaa agttcgcggc gtgggtccgt cgcgatctgc tcgtactcac
    18361 cgtgcccggc ttgatcatca tgctcggcgg cggctggtac ctgctgctgc gccaggggca
    18421 gctctggaat ccgacaggct acgtccgtca gctgcgcgtg cgccggacct tgatcgcgat
    18481 cgccgccgct ggtgcgatcg tcgcgctgcg cgccgtgtgg atcaccaaga ccggcacaca
    18541 cggcgaaggc ccgacgtggg cacagttcaa gtgcgagcac acgctcgcag cgatccgcgg
    18601 cccgctgtgg ggcatcgtcc atcacgtcgt ttatttcggg ccgatcatcc tcgtcgcgac
    18661 gatcgcgtgg cggcgcatcg cggcgctcgg cgcgtcgtgg ggaccatcgg cggtgctcgc
    18721 gctcgcactc gcactcgcgt tcgcagccgg ctcgaactcg cggcagtgga atcacctggt
    18781 gccgttcatc gtcgccgcga cgatcgcggt cacccacgag ctgtggacca cgcgcagagt
    18841 cgtcgagttt gcggcactga cgctcgcgtg gtcgaagttg tggctcgtga tcggctacga
    18901 caagcacgac aactggctga cgttcccgaa ccagcgctac ttcatgaatc agggaccgta
    18961 cgcgaacgac acgatgtacc tcgtgcacct gatcgcgtgc gcggtcacca cggggctgct
    19021 ctacgtactg ctgttccgat cacagagcgc tgtacaggct cacggtggtg ccgtcggcgg
    19081 tcacgagccg tagctcggtg gacgcctccg cgagcgacag cccggcgtcc acgccgtcgt
    19141 tgtagctcgc gatgtcgacg aggatctcga agttcgacgg atcgccgatg cacccgctga
    19201 cctgctcggc gcctgcgcga ctcgcgcacg gattttcgtc cgccaacaag accgcgacgt
    19261 ccgcggtgac gtgatcgctc gccgcatcgt acgtccagtg cgcgctattc gcagcgaccg
    19321 gcgtgtgatc gcagtagacg agtgccgatg cgtcgggatt gatccacgcg tgatcggagc
    19381 caccgtactc ccacgcgagc agcgggatca ccgggcgttg ttcggtgcca cacaacgcga
    19441 gcagccactg cgcgtcggcg tcggcggata cctgatcgac ggtcagcgcg ttgatgtctc
    19501 ctgcgctgcc gggatgcgcc gccttgtacg cgaggatgcg cgcacgaatg tcgtcggggt
    19561 tcatcgggtc gtcgtcgccg ctagacgttt gcgcgccaca gccgaccgcg agcagtgcca
    19621 ccaggatttg cgtccgcatg catgcgaccc tagagcatcg agatgaagcc tcgatgaagc
    19681 gctacatgca gacggcgcaa ttcggcagcg tcgagtcgca accatcggca acgctgcagc
    19741 ccgtcgggca cgtcacggtg ccggggtttt gcgggcaaaa cgcgatcgcc gcgcaggtga
    19801 cgtcgcactg acacgacgct gcacagtcga cgctgccctt gcagctgtcc atgccgctgc
    19861 agtcgacgtt gcacttgccg gcgccgcagt gattcgtgaa gccgcagctg ttcaggcccg
    19921 agcacgtgat gttgcagttc gtcgagttgt tgcaatgact gactgccggg catgcatccg
    19981 ggcccgaaca gttgagcgtg cagggcccag cgccgcactc gacaccgaaa tagcacgcgt
    20041 gatcaccgac gcagttcacg gtgcacgacg atgcgctgcc acacatgatg tcggccttgc
    20101 acgattgaaa gcccgtgcat gtgatgacgc atggaatgcc gggcgggcac acgatcgtag
    20161 tgagcgcgtc gccgtcgatg tggcactcgt tgttcgcgca gccgccgttg cactcggtcg
    20221 ggcacacgaa cggcacgtcg ccggtcgggc cgtcggtcac gagcgcatcg gtatcggcat
    20281 cgtcgccact gccgccggtg tcaccccgcg ccgacgcatc ttgctggcgc tcctcgtatc
    20341 cgaagcggcc gcatgccgcc aacgcacacg cggcgatcgc gacgagcaca ccccgagaca
    20401 gcactacttc cagactatca cgcgctacag cttcttgaag tagtcgccga ccagctcgaa
    20461 gtcgtggatg aagtcctctt tcgcgagccg tcgcagctcg tcgtcgggtg cgcgtaacac
    20521 cgacgccgga tgcacggtag cgaacgtcca cctagcgatg cctgtccctt cgatcacgtg
    20581 accgcgttct ttcgtcacgc gaaagctcgc tccgagcagc gcctgtgcag ccgttgcgcc
    20641 gagtaagacg acaatgtcgg gctcgaccac ctcgagctcg ctgcgcagcc acggtttgca
    20701 cgccgcgatc tcgtagcgcg tcggcttgtc gtgtaaccga cgcttacccc gctgcgtgta
    20761 cttgaagtgc ttcaccgcgt tcgtgacgta gacctcgtcc cgcggaatgc cgccgagcgc
    20821 g
//
LOCUS       DASUFX010000009        22504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_696003,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000009 DASUFX010000000
VERSION     DASUFX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696003"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFV99_00370"
     CDS             complement(<1..305)
                     /locus_tag="VFV99_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5057772.1"
                     /translation="MNKHRFVWHDLSTKDLAASKRFYGEIFNWKFDASANGPYTHITA
                     GDEMIGGIRQMDANEHQPPSWLGYVGVDNVAATVASITSAGGKVHMPTMTMENVGTFA
                     "
     gene            417..1898
                     /gene="hpnR"
                     /locus_tag="VFV99_00375"
     CDS             417..1898
                     /gene="hpnR"
                     /locus_tag="VFV99_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HEX5057773.1"
                     /translation="MKVLCVHPSGLMYNEIFLRLEPLGVELVAAAVRAAGHDVRLLDL
                     QAATHADYFRTVDEFRPDAVLIGMNYLANVPEVVDLCKATKARYPRTLTCVGGHSASF
                     TARELIKHGAGAIDCVARGEGEEIAPRLLEAWRDDPAKLHLLDGIVTAAGEGPPPKKI
                     HSLDTLRPARDLLPNRKKYFIGVLDPAASIEFSRGCPWDCAFCSAWTFYGRSYRQIDP
                     ELCAEDLASIREPGVFIVDDVAFIHADHGNAIADALERRRIRKRFYLETRGDVLMRNK
                     DVFARWKRLGLEYMFLGIEAIDAEGLKAFRKRVSIDKNFEALECARSLGITVAINIIA
                     DPDWDAARFAVIREWALSVPEIVNISVNTPYPGTETFASSARVLTTRDYRLFDIQHAV
                     LPTRLPLPMFYAELVKTQQVLNRKHLGLSALASTAAISARLLLKGQTNFIKMLWKFNS
                     VYNPERQLADHARPVKYEMDLPMSTSAAHPARPRHRLFVLQPG"
     gene            complement(1895..2470)
                     /locus_tag="VFV99_00380"
     CDS             complement(1895..2470)
                     /locus_tag="VFV99_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057774.1"
                     /translation="MPFQLIKGMTATADGIDKGLLDAGDLQTRFATRQELLAAASNPE
                     VAEEVSVEFVEEALARGDECYGIFDGPTLVSFGWYSNHPSQFSDTLDLHFDRAWMYMY
                     KGYTLRSHRGKRLHGIGMSRALYAYAERGYRGLISYVRSTNFQSLRSTERMGYQIFGE
                     IYIAKAMGHALVWASPGCAAYKFSIERRRSR"
     gene            complement(2723..3124)
                     /locus_tag="VFV99_00385"
     CDS             complement(2723..3124)
                     /locus_tag="VFV99_00385"
                     /inference="COORDINATES: protein motif:HMM:NF022443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HEX5057775.1"
                     /translation="MRRRFAWLGAAAIALGIAVLVYRGPGRAIVRGHVGDVAATMLVY
                     AALGAAWRTRRTVRATMTFAVAVAIELVQIGWHARSPAGELLIGSTFDPWDLVAYALG
                     VAIAVGWDVAASRRGRSTFDHVSSRSSIARG"
     gene            3400..4536
                     /locus_tag="VFV99_00390"
     CDS             3400..4536
                     /locus_tag="VFV99_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5057776.1"
                     /translation="MQPKRILILGGTGFLGPKTIDAALARGHSITIFNRGKREKIQPL
                     EQKVDHLFGNRDPKLPADEANPDGSPVGLTQLEGKTWDVVIDNSGYFPRHVEASAKLL
                     AANAERYIYISSISCYAEPYPPAGGDETMPLAELKDPTVETMGDQFENYGGLKVLCER
                     AASAAFGAERTAIVRPGYIVGPGDPTDRFTYWPARIAAGGDVLAPGSPDDPLQWIDVR
                     DLAAWLVLLAERGTSGTFNAVGPETPARWGTVLDTCVTASGNKAKLTWVPVDWLDKHG
                     MGGDESFPIWAPAVGKTAGFHRWKNARAREAGLTFHSIDDTVRSLLAWFPTELERRAR
                     VTRELTAAAEAKGQPPPKLADPTVWRAGPPREREAELLAEFAKR"
     gene            complement(4533..6743)
                     /locus_tag="VFV99_00395"
     CDS             complement(4533..6743)
                     /locus_tag="VFV99_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5057777.1"
                     /translation="MKRALLVVVAACGGSKPTPQPTAQAPAERQPEPMPAVQEAPKPS
                     TLNGPPVAEVRVVKDVLHGVTVEDPYRWLEADDAAVKKWSDAQNVYARGILDKLPEVE
                     TLRNEITAIMKAPITQYGGFRVAGSHIYGFRKQPDKEQGELIVFDDPEKVADAKVVVD
                     PTAGGNAHRSIDWMEPSPDGSKVAVSISELGSEKGDLHIFDRGGKEVDVVIPNVNRGT
                     GGGDAAWTPDGKGIYYTRYPAKGEKPDGEADFWMQAWFHQLGTPLEKDHYELGKDFPK
                     IAELVLETDKKGRVLAKIQNGDGGEFRHYLRDAKGTWRQLDDWKDGITYLGFGTTDDL
                     WAVSIKDAPNGKVLSIPANAKSAAEGKVVIAEGKDSILTEFFQSDTGVTDGGDRIYLL
                     YLVGGPMELRAFTRAGKAAKSPALPPVSSVGKPVLLKHGGLVGSTSFTMPFAWYRFDT
                     KTGAAKIVPAISPKSPVDLSGFEARRELATSKDGTKIPYTVIWPKGAKQDGSTPCIAT
                     GYGGYNISQTPYFATWIAPMLTRGFCFVETNLRGGGEFGQAWHRAGMLTAKQNVFDDF
                     AAVLQALIANKYTSTPRLGIIGGSNGGLLMGATFVQHPELMKAVVAEVAIFDSVRAEE
                     STNGEYNVTEFGTVKDEAQFTALLAYSPYHHVAKTSYPAILMTTGDHDPRVPPWHSRK
                     MTARLQAAQTAKAPILLRTSSNAGHGVGSSMSEQIDLRTHIFAFLLDQLKPTER"
     gene            complement(6778..7233)
                     /locus_tag="VFV99_00400"
     CDS             complement(6778..7233)
                     /locus_tag="VFV99_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057778.1"
                     /translation="MDLEATLKELGVDKSAAKVVGLLPLVYVAWADGKVQKAERESIF
                     HAANEMGWLAGGGDAVLERWLTTRPTEAQLETGIALLNYLAKDKTHTDIDADDLQMLL
                     LLCKDVADAAGSFLGIGRAQSREEIAALESIASSLDIKSAKSWRALQSA"
     gene            7248..8081
                     /locus_tag="VFV99_00405"
     CDS             7248..8081
                     /locus_tag="VFV99_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057779.1"
                     /translation="MRRVVAMLTWLAFAACDRSAPAPAQPPSSQPATGSAAAVVPADA
                     ALAQMPVDAEVLAQPNRPLTAGEIAMLEPIFKDSLDYKAVRVINNSFPFQPENVYMTP
                     RGHVYAPGGLWQDDFSIGVLHRAVFVHEMTHVWQYANGMDLIAQSLVEFSKNRGDYEK
                     AYAYDLDAARDLTDYGLEQQASIVEDYFMITSELTEPLRIKNRGLTHQQRDTLYAAVL
                     KQLITNPRYARNLDAKQIAAQHAATSEKTKPGPAACKESEAEHGTAHMCSWRFEPTAK
                     H"
     gene            8118..8954
                     /locus_tag="VFV99_00410"
     CDS             8118..8954
                     /locus_tag="VFV99_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057780.1"
                     /translation="MDLDLDMYWCAVKGAVSPVELVPRLYGPHATVRAPTADETVLGR
                     TDGERKKRVASWFTKGEWTFFLSPRDIFSVEKQLPECEVLTLHCDARNEWTSIAHWVN
                     GVSDWSAYGSDEDEWVRVEGELPASVAKYGSMTEPDRVSVPIKIGAELTGFRHDKHAR
                     PRGFQIVDIQPPRTLIQVDGTSVTNPTLSQVEAAVETYLTAAAPLVLTVGDGEAAATL
                     TTRRVDEKVHSIEHQQRSHRSVARVSWGKRPIDALDVRRCFRTFYDEGETSDHCSWSP
                     VA"
     gene            complement(8927..9310)
                     /locus_tag="VFV99_00415"
     CDS             complement(8927..9310)
                     /locus_tag="VFV99_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057781.1"
                     /translation="MIKKAGALERKLEEGRVLAEQLRAEAEHLRESQELARQRSEASR
                     VHAELGRADSEETRELREDMRRGGERLRRAKTQREQTSLTGNGEEHATTSEISPALRR
                     AIREEVRAALTQLNAKHQATGDHEQ"
     gene            complement(9627..10277)
                     /locus_tag="VFV99_00420"
     CDS             complement(9627..10277)
                     /locus_tag="VFV99_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057782.1"
                     /translation="MKGWMFVVIAVGGCVDEDASRLGTQEQGLTEGQVNALNNVGRTT
                     GVTYQLIDNPDLVAPFLVDNPDLIDNPDILPPLLQLQFRPVSDLLRISEYLDGSGQLL
                     GRVASGDPHMSCQGFTSTTIATMKGGAGSWNVSLRDRAGTSLARLAPGEQAPPDPDRP
                     GEQTPPDPERPGDVTPPDPDRVTISIRLTGAATVNIYDANGNLIQAVHGGVIRTGP"
     gene            complement(10274..11509)
                     /locus_tag="VFV99_00425"
     CDS             complement(10274..11509)
                     /locus_tag="VFV99_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5057783.1"
                     /translation="MHPELEATMARGRAAGVTHRVARERDRNGRTLRLEPEGDVVSFA
                     TCSYLGLERDERLAQRAKAAIDRFGVSFSASRCFVTSALYAEAEELLAAIFGRPVVLA
                     GSTTLAHGAALPILVGARDVVLLDRQVHHSVQTAVAALGARRARVPVAHSDLDALEAA
                     IKEAVANDAHHIWYCADGIYSMFGDRLPIAGLSELMARYPQLYAYLDDAHGMSWCGTH
                     GAGSLIDAPLPRERTVIATSLSKGFGCAGGVLAVPDVATKHRIENLGPSLMFSIQLPP
                     PVLGAICASARIHLGDEIFSLQATHRDRRAFARALVTSHPIVGPRAVKIDGEPTPVQY
                     VVLGTADDAIEATARLLDLGFLVNPVAFPAVPRDAGGIRLTITRCHTTSDIQRLVAAI
                     GDVVHSMQPDRLLNMKESA"
     gene            complement(11563..14442)
                     /locus_tag="VFV99_00430"
     CDS             complement(11563..14442)
                     /locus_tag="VFV99_00430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057784.1"
                     /translation="MADTSEPEASCIDENVLVELAEGKLDDTARAAIDAHIDHCAVCR
                     ALVAASQPTLPVADSMPAGASQQPRPRGSTLARYLILDVVGAGGNGIVYAAYDPELDR
                     KVAVKVLRTEGADDGESRRSRTLREAQAMAKLSHENVVSVYDVGEEDGKVFVVMELID
                     GSTLTRYCEEPSRSWREIVGVFSRAGRGLAAAHAAGLVHRDFKPENVLIARDGRVLVT
                     DFGLARVLDPATSASMSAAPGDLARALPTHPLTRTGAIAGTPAYMPLEQLRGEPLDAR
                     SDLFSFCVALYETLYRQRPFAGDSIEELAKQIEDDRVQPPPRASRVPAWVHRILVRGL
                     SSRPEARFSSMAELLAALGRDPARLRVRALAAVGIVAVSVSAGAFMWRSHSDAQLVCQ
                     GADRALDDVWNVPRQQAMRIAFGATGRPFADATFGKVSAAFDRYARDWTDMRVRACKA
                     TRVSREQSEELMGLRMQCLDHRLDEMRALIALLMTPDAATLDEATRAAYKLTPLVECA
                     DAEALTAPVRPPRNPAVRIQVERLRVELAAAKALRDLGRYREQAERSAKLVAEAKAIG
                     YKPLEADAQLLLGKALEINADYAHAADALHEAVWAAEAGRDLKLAVRARTELVWTVGH
                     QLGKPELGAEYARDAEALLSGLGSDPELEAQVVAMQSDAAWEQGREEESMKLAKRVVD
                     ANRTLYGDVHPQTAIAMVNLAVGYSEWQRHADALPLYQQALAIYEQTLGTHHPLYAHT
                     LFNVADAELGLARYSEARVDAERALALIETALGPEHALVASASAVLGLVAQHESNYEE
                     AERHHARALALAEKLRGPRHGLVARYAGYLAASADAQGHHGQALELYRRARSIYEELG
                     NELLLARTDSAIGQQQLASGALTDAVKTLERALRVQEPKDKSAPDLASTRFALARTLW
                     SLPGGDRARATSLAKAAQAGYAEAGPGAASARADVAQWLDQHEVP"
     gene            complement(14435..15358)
                     /locus_tag="VFV99_00435"
     CDS             complement(14435..15358)
                     /locus_tag="VFV99_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057785.1"
                     /translation="MVRSRNSRYREAVAERLVGVFLERWKGEVRDVPTALQSGLEELV
                     SRGKQRWPSVALESIAFVRHLGDHAPTDTDPVTAVARVRAEELYLACACANGCPEAVR
                     ICDTELVHKLRPHIARLRADPGFTDEALQAVRDRLFVSSQAAPPKIAQYRGQGALDSW
                     IRMVGLRTALNLIASERRHDALDDDHLSTMLQPGDDPELDYIKARYRAAFVAAFKHAF
                     SQLPQRERNMLRFQHLDGLTPERIGGIYGVHRTTAMRWIAAAHKQMLDETRRILMQQL
                     SITASECESLFDLVQSRLDITLRSLLKTTHG"
     gene            complement(15415..16308)
                     /locus_tag="VFV99_00440"
     CDS             complement(15415..16308)
                     /locus_tag="VFV99_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5057786.1"
                     /translation="MNRRLLAIYGAIAANLAIATMKFVAAGVSGSSAMLTEGIHSLVD
                     TGNGLLVLLGVRLARRPASPEHPYGHGLETYFWTLVVAMSIFGVGGGVSIYQGILHIG
                     TPRPLESTLLSYGVLAGAFVFEGTSWLLGYRGFKGACSELGVWEGIRRSKDPTTFVVL
                     LEDSAALAGLTVAALGITLARWLDIPELDGAASVVIGVLLVVVAVILARECRSLLVGE
                     SAEHTLVAKVRQIAQHDPAVAEASLPRTMHIGPDMVHVDLDIRLQGDLSTSDIVEATH
                     RIEDAVRAAHPRIRRLFVRLV"
     gene            complement(16396..17568)
                     /locus_tag="VFV99_00445"
     CDS             complement(16396..17568)
                     /locus_tag="VFV99_00445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX5057787.1"
                     /translation="MRATCWMGKKHVSVEHVPDPKIINNRDAIIRVTATTICGSDLHL
                     YNGYMPTMQKGDILGHEFMGEVVELGRGVTNLKVGDRVVVPFAIACGNCEQCELGMFS
                     CCENGNPSGWMPEKLMGHPTCGAFGYSHLTGGFSGGQAEYVRVPYADFGPLKVPRHLP
                     DEKVLFLSDIFPTGYMGAEFCEITPRKVIAVWGAGPVGQFAIASAKMLGAERVIAIDR
                     YPYRLKMAIEKAGATDAINYEDQNVQEVLRDLTAGRGPDACIEAVGLEAHGHGVQYVH
                     DAMKQKVKIQMDRPLAVREAIMACRSGGIIAILGVYAGFVDKFPLGALMNRGLTIKTG
                     QCHVHRYMKPLLERIERGDIDPSFIVSHYMNLDDAPKGYDMFMHKQDEVMKIILRS"
     gene            complement(17565..18539)
                     /locus_tag="VFV99_00450"
     CDS             complement(17565..18539)
                     /locus_tag="VFV99_00450"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5057788.1"
                     /translation="MKSVSAFRSPAREDQHDRFATKLSAGLGWFSLALGTAQLAVPRT
                     LSRLIGIEPSIAASTILRGLGIREIASGLGLLLEPRRPVPLWARVAGDVIDLGLLGIA
                     ASSHRTNRVRLAGAMASVAGVMALDVIAARGTQRHFEAANRPVIYSVTINRPVQEVYQ
                     AFRDFSRFPQFMDYLASVQELDRVRSRWVAKLPIGGTVEWDAEITDEMPNQRIAWQSV
                     EGSRIKTRGSVIFSTAPSRNSTEVRVEMQLGFTGKAPSTLLAKFFAKPQIKGDLRRFK
                     QLVETGEVLFSDATEFRRPHPAQPPEQVQRRPKIFEPRRPTAEKGARP"
     gene            complement(18739..19140)
                     /locus_tag="VFV99_00455"
     CDS             complement(18739..19140)
                     /locus_tag="VFV99_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057789.1"
                     /translation="MGPTGVALYIDSPWTLGLLVNQLWSVADTSDVNAPDVSQMFIQP
                     FVSYTKHKWTLTAETETTLNWEANDDKVTAPLELVGARLTKIGFLPLSVQVGAGWFAA
                     SPDGGPEWRLRLDFVILLPDPKVLKAGLMKK"
     gene            complement(19192..19848)
                     /locus_tag="VFV99_00460"
     CDS             complement(19192..19848)
                     /locus_tag="VFV99_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057790.1"
                     /translation="MVLLAGCDLLFQIDDLTGHTDAGGSADSVDATQDMHTTNCVADD
                     FAMLGTQWTMFENPPTAVVAIDGTLSISLNKAADDHGGIDSAVRDFTGASIEVDVVTV
                     PDTSSETYMEWDRGHDWYSIAVDHGELSYGYSVDGSDRTIEIPYEPDKHRRFKLAHDR
                     QANLIRMSVRNSLGEWTELGTVPVSIPMTALTVELASGSYTAALTSGVARFDNFVTCF
                     "
     gene            complement(20000..>22504)
                     /locus_tag="VFV99_00465"
     CDS             complement(20000..>22504)
                     /locus_tag="VFV99_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5057791.1"
                     /translation="LLQIFPVLRGVAALDVAARLDRARDPGAAEIDQRSLAFAALADL
                     LAAVAARRPVVLMLDDIHWADADSLALLGELFAGAGAPPVLLVATARVSDDGVIAARN
                     AMRVAVEVIQLGGLARDEAEALVRATSPDADAARLANDAGGHPMFLTELARRSETSGL
                     LNLRLDDALWQRIGELDAGARALLEVVTLGAAALPLGVVSAAAGLAADDVARDLGALR
                     AARLVRIIGGRDHDSDDGSVRAARGFASLVEPYHDRVRETVAARVPAERRQSIHRAIV
                     SSLEAAGGGVEVLTYHLAQAGEGARAGALAEVAAQHALDALAFDRAAEWMRITLELGE
                     LTPDRRRALIAERADVLARAGRTAEAADAFLSAAEGESIDAARELRRRAAEQLLIGGH
                     LARGRELAIELANEIDLSLPGSTASAITRLIWYQTRLRFSRLRWPHRSPEEVTERNRI
                     RIDVSWSLATGLSMVDSTRGACSAMRLPLLAMPAGDPTRIARAMCAAAVAASGMGRRR
                     LAERLRDATHRSAAESDDPSAAPYAAFADVAYLFYLINDWQGAAAACERAASLLPDRR
                     SHTFEGDIIEQHHVWSLSILGELKELRRRVPVTIRSAQRIGNRFVEVSHRTFFAILHL
                     LVDRPDEALADLRDALAGWHTEGIGDDVTNPFFFALKNRTLIALYQRNPDSDPTLDAD
                     WARARASLLYQIPMIRLECTQFAAQLEVGRASLARARGDAAGCKRYLAKARRWIARTR
                     IGLQAGTHLADLVAASAALVAGDLDDAAARFTALVEVLGAGGQRALAAACRWRLAAII
                     GGSAGDAHRAEADRFFADEGVLRPDRLVDGLLPGWV"
BASE COUNT         3790 a         7440 c         7401 g         3873 t
ORIGIN      
        1 gcgaacgtgc cgacgttctc catcgtcatc gtcggcatgt gcaccttgcc accggcgctg
       61 gtgatgctcg cgaccgttgc cgcgacgttg tcgacgccga catagccgag ccagctcggc
      121 ggctggtgct cgttcgcatc catttgccgg atgccgccga tcatctcgtc gccggcagtg
      181 atgtgcgtgt acgggccgtt cgcgctcgcg tcgaacttcc agttgaagat ctcgccgtag
      241 aacctcttcg aggctgcgag gtccttcgtg ctgaggtcgt gccagacaaa gcggtgtttg
      301 ttcatgcgcc gacgctacac gggagtgcgc ggccacgcct gcgcgtgact acgtcatgcg
      361 gacgtcctcg taccgggagc gcgccatcgc gccgctgctt gcatgtgttg gtcggaatga
      421 aggtgttgtg tgtccacccg agcggcctca tgtacaacga gatcttcctt cgcctcgagc
      481 cgctcggggt ggagcttgtt gccgcggctg tgcgcgcagc cgggcacgac gtgcgcctgt
      541 tggatctgca ggcagcgacg cacgctgact acttccgcac cgtcgacgag tttcgccccg
      601 atgccgtgct catcgggatg aactatctcg cgaacgtccc ggaggtcgtc gacctttgca
      661 aggcgaccaa ggcgcgctat ccgcgcacgc tgacgtgcgt cggtggccac agcgcgtcgt
      721 tcacggcccg cgagttgatc aagcacggcg ctggcgcgat cgactgcgtc gcgcgtggtg
      781 aaggcgaaga gatcgcacct cggctgctcg aggcgtggcg cgatgacccg gccaagctcc
      841 atttgctcga cggcatcgtc accgcggcag gcgaggggcc gccacccaag aagatccatt
      901 cgctcgacac gctgcggccg gcgcgcgacc tgttgccgaa tcgcaagaag tacttcatcg
      961 gcgtgctcga tcctgcggcc tcgatcgaat tctcgcgcgg ttgcccatgg gactgcgcgt
     1021 tctgtagcgc gtggacgttc tacggccgca gctatcgcca gatcgatccc gagctgtgcg
     1081 ccgaggatct cgcgagcatt cgcgagcccg gcgtgttcat cgtcgacgac gtcgcattca
     1141 ttcacgcaga ccatggcaac gcgatcgcag acgcgctcga gcgtcgccgc attcgcaaac
     1201 gcttctatct cgagacgcgc ggcgacgtgc tcatgcgcaa caaggacgtg ttcgcacgct
     1261 ggaaacggct cgggctcgag tacatgtttc tcggcatcga ggcgatcgac gccgagggcc
     1321 tcaaggcgtt tcgcaagcgc gtctcgatcg acaagaactt cgaggcgctc gaatgcgctc
     1381 gctcgctcgg catcaccgtc gcgatcaaca tcatcgcgga tcccgactgg gacgcagcgc
     1441 gctttgcggt gattcgcgag tgggccctgt ccgtgccaga gatcgtgaac atttcggtca
     1501 acacgccgta ccccggcacc gagacgtttg cgtcgagtgc gcgcgtgctg acgacgcgtg
     1561 actatcggct cttcgacatc cagcacgctg tgctccccac tcgcttgccg ctgccgatgt
     1621 tctacgccga gctcgtgaag acgcaacagg tgctcaaccg caagcatctc ggcctctccg
     1681 cactcgcttc gaccgccgcg atctcggcgc gcctgctcct gaaggggcag acgaacttca
     1741 tcaagatgct gtggaagttc aactcggtct acaacccgga acgtcagctc gcggaccacg
     1801 cgcgtccggt caagtacgag atggatctgc cgatgtcgac gagcgcggca catccggctc
     1861 gtccgcgaca tcggttgttc gtgctgcaac cgggctaacg cgagcgacgg cgctcgatgc
     1921 tgaacttgta ggcggcgcag ccaggcgatg cccagaccag cgcatgcccc atcgccttcg
     1981 cgatgtagat ctcgccgaag atctgatagc ccattcgttc ggtcgaacga agcgactgaa
     2041 agttcgtcga gcgcacatac gagatgaggc cgcggtatcc gcgctccgcg taggcgtaga
     2101 gcgcccggct cataccgatc ccatgcagtc gctttccgcg gtgcgacctg agcgtgtagc
     2161 ccttgtacat gtacatccac gcgcgatcga agtggaggtc gagggtgtcc gagaactgcg
     2221 aggggtgatt cgagtaccaa ccaaacgaga ccagcgtcgg gccatcgaag atgccgtagc
     2281 actcatcgcc tcgtgccagc gcctcctcga cgaattcgac gcttacctct tcggccactt
     2341 cggggttcga tgctgcggcg agcagctcct gacgtgtcgc gaaccgagtc tgcaagtcgc
     2401 cggcatcgag caagcctttg tcgatcccat cggctgtggc cgtcatccct ttgatcagtt
     2461 gaaagggcac gagccggttc acgatccggt gctggagctc gtgaagcgcc gcccgccgtc
     2521 caaagctgct cgcgatttcg ctcgtgtgtt tcaacatcgc tcgcaccggg gcaaggactg
     2581 cggccggatg ctcgtcgctg cgagaagtcc tcatgtgccg cgagagcagc aagaagatgg
     2641 ccatcagcga ccacgcatta ccaaggtctt atcgtgctgc gtcggttcca atggagcgcc
     2701 gacgcgcgtg gcgtgccgac ggtcatccac gagcgatcga cgagcggctc gagacgtgat
     2761 cgaaagtaga gcgcccccgc cgcgatgccg cgacatccca gccgacggcg atcgcaacgc
     2821 cgagcgcgta tgcgacgagg tcccacggat cgaacgtcga gccgatcaag agctccccgg
     2881 ccggcgaacg cgcgtgccag ccgatctgca cgagctcgat cgcgacggcc acggcaaacg
     2941 tcatggtggc gcgcaccgtg cgccgcgtgc gccacgccgc accgagcgcc gcgtacacga
     3001 gcatcgtggc ggcgacgtcg ccgacatggc cgcgcacgat cgcgcgaccg gggccgcgat
     3061 acacgagcac ggcgatcccg agcgcgatgg ctgcggcgcc gagccaggcg aaccgtcgtc
     3121 tcatgcagat gcaacgcgcg acgaccgcgg cttcgtccat cgcgtgacca ccgatcgcga
     3181 aagtgttggc gaaataattc gtcggtgcga cgccgtgcga cgcgtgcgac gtcgagcgac
     3241 cgcgcgtaca gacacggtaa cgtcggctcg atgtcgagct ctcgtcgtac gctcacgcgc
     3301 cgtgactggc ttcgcggtgc cctggctaca tctgcactgg ctgcctgcgg tggcggccga
     3361 tcgtcgtcac ccaaaccgtc gacgccaacg gagaccaagg tgcaaccgaa gcgcatcctg
     3421 attctcggcg gcaccgggtt tctcggcccg aagacgatcg acgccgcgct cgcacgcggt
     3481 cactcgatca cgatcttcaa tcgtggcaag cgcgagaaga ttcagccgct cgagcagaag
     3541 gtcgaccatc tgttcggcaa ccgcgacccg aagctgcccg ccgacgaggc gaatccagac
     3601 ggcagtccgg tcggcctcac gcagctcgag ggcaagacgt gggatgtcgt gatcgacaac
     3661 tccgggtact tcccgcgcca tgtcgaggcg tcggcgaagc tgctcgcggc gaacgccgag
     3721 cgctacatct atatctcgag tatctcgtgc tacgccgagc cgtatccgcc cgccggcggc
     3781 gacgaaacca tgccgctcgc cgagctcaag gatccaaccg tcgagacgat gggcgatcag
     3841 ttcgagaact acggtggcct caaggttctg tgcgagcgcg ccgcgagcgc ggcgttcggc
     3901 gccgagcgca cggcgatcgt tcggccgggc tatatcgtcg gtcccggcga tccgaccgat
     3961 cgcttcacgt actggcccgc gcgcatcgca gccggcggtg acgtgctcgc gccgggctcg
     4021 cccgatgatc cgctgcaatg gatcgacgtg cgcgatctcg ccgcgtggct cgtcttgctc
     4081 gcggaacgcg gcacgtcagg cacgttcaac gccgtcggtc ccgaaacgcc cgcgcgctgg
     4141 ggaaccgtcc tcgacacgtg cgtgaccgca tcgggcaaca aggcgaagct gacgtgggtg
     4201 ccggtcgact ggctcgacaa gcacgggatg ggcggcgatg aatcgttccc gatctgggct
     4261 ccggcggtcg gcaagacggc cggctttcat cgctggaaga atgcgcgtgc gcgcgaggcc
     4321 ggcctgacct tccattcgat cgacgacacc gtgaggtcgc tgctcgcgtg gttcccgacc
     4381 gagctcgaac gacgcgctcg cgtcacgcgc gagctcaccg cggcggcgga ggcgaaaggc
     4441 caaccgccgc cgaagctcgc tgatccgacg gtgtggcggg cagggccgcc gcgcgagcgc
     4501 gaagccgagc tactcgccga gttcgcgaag cgctaacgtt ccgtcggttt cagctggtcg
     4561 agcaggaacg cgaagatgtg ggtccgcaga tcgatctgct cgctcatcga gctgccgacg
     4621 ccatggcccg cgttcgacga ggtgcgcagc aggatgggcg ccttggcggt ctgggcagcc
     4681 tggagccgcg cggtcatctt gcgcgagtgc cagggcggaa cacgcgggtc gtggtcgccg
     4741 gtcgtcatca ggatcgccgg ataggatgtc ttcgcgacgt ggtgatacgg cgagtacgcg
     4801 agcagcgccg tgaactgtgc ctcgtccttc accgtgccga actcggtcac gttgtactcg
     4861 ccattcgtcg attcttcggc gcgcaccgag tcgaagatcg cgacttccgc gacgacggct
     4921 ttcatcagct cggggtgctg gacgaacgtt gcgcccatca gcaggccgcc gttcgaaccg
     4981 ccgatgatcc cgagccgcgg tgtcgacgtg tacttgttcg cgatcagcgc ctgaagcacg
     5041 gccgcgaagt cgtcgaacac gttctgcttc gcggtcagca tgccggcgcg atgccacgcc
     5101 tggccgaact cgccaccgcc acgcaggttc gtttcgacga aacagaaccc gcgcgtgagc
     5161 atcggcgcga tccaggtcgc gaagtacggc gtctggctga tgttgtatcc gccgtagccg
     5221 gtcgcgatac acggcgtcga accatcctgc ttcgcgcctt tgggccagat caccgtgtac
     5281 gggatcttgg tgccgtcctt cgaggtcgcc agctcgcgcc gcgcttcgaa gcccgatagg
     5341 tccaccggcg acttcggcga gatggccgga acgatcttcg ccgcgccggt cttggtgtcg
     5401 aaccggtacc acgcgaacgg catcgtgaac gaggtcgagc cgacgagccc gccgtgtttt
     5461 agcaacaccg gcttacccac cgacgacacc ggcggcagtg ccggcgactt cgctgctttg
     5521 cccgcccgcg tgaatgcgcg cagctccatc ggtccgccga ccaggtacag caggtagatg
     5581 cggtcaccgc catcggtgac gcccgtgtcg gactggaaga attcggtgag gatcgagtcc
     5641 ttgccttccg cgatcacgac cttgccctcg gccgccgact tcgcgttcgc cgggatcgac
     5701 agcaccttgc cgttcggcgc gtccttgatc gagacagccc acagatcgtc ggtcgtgccg
     5761 aaaccgagat aggtgatgcc gtccttccaa tcgtcgagct gacgccacgt gcccttcgca
     5821 tcgcgcaggt agtggcggaa ctcgccgccg tcgccgttct ggatcttcgc gagcacgcga
     5881 cccttcttgt cggtctcgag cacgagctct gcgatcttcg ggaagtcctt gccgagctcg
     5941 tagtgatcct tttcgagcgg cgtgccgagc tggtggaacc atgcctgcat ccagaagtcc
     6001 gcttcgccgt ccggcttctc gcccttcgcc gggtagcgcg tgtaatagat gcccttgccg
     6061 tctggcgtcc atgcagcgtc gccgccgcca gtgccgcgat tgacattcgg gatgacgacg
     6121 tcgacctctt tgccgccgcg gtcgaagatg tgcaggtcgc ccttctccga gccgagctcc
     6181 gagatcgaga ctgcgacttt cgagccgtcc ggcgacggtt ccatccagtc gatcgagcgg
     6241 tgtgcgttac cgccggcggt tggatcgacg acgaccttcg cgtccgccac cttctccgga
     6301 tcgtcgaata cgatcagctc gccctgttcc ttgtccggct gcttgcgaaa cccgtagatg
     6361 tgcgagccgg caacacgaaa cccgccgtac tgcgtgatcg gcgccttcat gatcgcggtg
     6421 atctcgttgc ggagcgtctc gacctcggga agcttgtcga ggatgccgcg cgcgtacacg
     6481 ttctgcgcgt cggaccactt cttcaccgcg gcatcgtcgg cttcgagcca gcgatacgga
     6541 tcttcgacgg tgacgccatg aagcacgtcc ttcacgacgc gcacctccgc gacgggcggg
     6601 ccgttcagcg tcgacggttt cggcgcctcc tgtacggcag gcattggttc gggctggcgc
     6661 tcggccggag cctgcgctgt cggttgaggt gttggcttcg aaccaccaca cgcggcaacg
     6721 acgacgagaa gtgcacgttt catagcccga gtcgtagcac tcggtggctg cgacccgcta
     6781 ggcggactgc aacgcgcgcc agctcttggc actctttatg tcgagcgacg aagcgatcga
     6841 ctcgagcgct gcaatctctt cgcggctctg cgcgcggccg atgccgagaa agctcccggc
     6901 cgcgtcggcg acgtccttgc agagcaagag cagcatctgc agatcgtcgg cgtcgatatc
     6961 ggtgtgcgtt ttatctttcg cgaggtagtt gaggagcgcg atgccagtct cgagctgcgc
     7021 ctcggtcggc cgcgttgtca gccagcgctc gaggaccgca tcaccaccac ctgccagcca
     7081 acccatctcg ttggccgcgt ggaagatgga ttcgcgctcg gctttctgaa ccttgccgtc
     7141 ggcccacgcc acgtaaacga gtggtagcag gccgacgact ttggctgcac tcttgtcgac
     7201 accgagctct ttgagcgttg cctcgaggtc catggcagta tggtgccatg agacgggttg
     7261 tcgcgatgct gacgtggctc gcgttcgcgg catgtgatcg ttcggcgccg gcgccggccc
     7321 aaccaccgtc atcgcagccc gccaccggtt ccgccgccgc ggtcgtaccg gccgacgcgg
     7381 cgctcgcgca gatgcccgtt gatgccgagg tgctcgcgca gccgaaccgt ccactgacgg
     7441 cgggcgagat cgcgatgctc gagccgatct tcaaggacag cctcgactac aaggccgtgc
     7501 gcgtcatcaa caactcgttc ccgttccagc ccgagaacgt ctacatgacg ccgcgcggtc
     7561 acgtgtacgc gcccggcggg ctgtggcaag acgacttctc gatcggggtt ctccatcgcg
     7621 ccgtgttcgt gcacgagatg acgcatgtct ggcagtacgc gaatgggatg gacctgatcg
     7681 cccagagcct cgtcgagttc agcaagaatc gcggcgacta cgagaaggcg tacgcctacg
     7741 acctcgatgc ggcgcgcgac ctcaccgact acggcctgga gcaacaggcg agcatcgtcg
     7801 aggattactt catgatcacg agcgagctca ccgagccgct gcggatcaag aatcgcggtt
     7861 tgacgcacca gcagcgcgac acgctctacg cggcggtgct gaagcagctc atcacgaacc
     7921 cgcgctacgc ccgcaacctc gacgcaaagc agatcgcggc acagcacgcg gcgacgtcgg
     7981 agaagacgaa gccgggtccg gcggcgtgca aggagtccga ggccgagcac ggcaccgcgc
     8041 acatgtgcag ctggcggttc gagcccaccg cgaagcacta gcgcgatcag cgcatcgcga
     8101 cgtgttatga cgcccgcgtg gacctcgatc tcgatatgta ctggtgcgcg gtcaagggcg
     8161 cggtcagtcc cgtggagctc gtgccacgtc tatacggtcc gcacgcgacc gtgcgtgccc
     8221 ccaccgccga cgagacggta ctcggccgca cagacggtga gcgcaagaag cgcgtcgcct
     8281 cgtggttcac gaagggcgag tggacattct ttctgtcgcc gcgcgacatc ttctcggtcg
     8341 agaagcagtt acccgagtgc gaggttctga cactccactg cgacgccagg aacgagtgga
     8401 catccatcgc gcactgggtg aacggtgtga gcgactggtc ggcgtacggc tccgacgagg
     8461 acgagtgggt gcgcgtcgaa ggcgagctgc cggcgtccgt cgcgaagtat ggttccatga
     8521 ctgagcccga tcgggtgtcc gtgccgatca agatcggcgc cgagctcacc gggtttcggc
     8581 acgacaagca tgcacgcccg cgcggctttc agatcgtcga catccagccg ccgcgcacgc
     8641 tgatccaggt cgatgggacg tcggtgacga acccgacgct gagtcaggtc gaggcggccg
     8701 tcgagacgta cctgacggcc gccgcgccac tggtgttgac agttggcgac ggagaggccg
     8761 ccgcgacact caccacacgg cgtgtcgatg agaaagtgca ctcgatcgag caccagcagc
     8821 gcagccatcg cagcgttgct cgtgtgtcct ggggcaagcg accgatcgat gccctcgacg
     8881 tacgacgctg cttccggacg ttctacgacg aaggcgaaac gtcagatcat tgctcgtggt
     8941 cacccgtcgc ctgatgtttc gcattcaact gcgtcagcgc ggcacgcact tcctcgcgga
     9001 tcgctcggcg gagcgctgga ctgatctcgc tcgtggtggc gtgctcctca ccgttccccg
     9061 tcagtgacgt ctgctcccgc tgcgtcttcg cccggcgtag ccgctcccca ccgcgccgca
     9121 tatcctcacg aagctcgcgc gtttcctcgg agtcagcgcg gcctagctcg gcatggactc
     9181 tcgatgcttc ggagcgttga cgtgcgagct cttggctctc acgcaggtgt tcggcttctg
     9241 cgcggagttg ttccgccagc acgcgccctt cctcgagctt gcgctcgagc gcgccggctt
     9301 tcttgatcat ctcgtcacgt tgaaggcgcg cttcgtcagc ctcgccccga ccgcgttcgg
     9361 cggtcgcacg tcggcgctcg gctgtctgac gccctgcttc cgccgtccgg cgcgtcgact
     9421 cggcgcgctt gcgcgtggtc tccgagttac gccgaggtac gcgttttctt ccgtccgcac
     9481 gagcagcagg catcggttct ctcccaaagc agccgatccg aactgtggca ggtccggtga
     9541 accgagcacc cacgaagtaa cgcggaggtg gcggcagata gcgagcgctt ttgcaccgct
     9601 cccgccgagc ggtagtctcg cgagcctcag gggccggtgc gaatcacccc gccgtgcacg
     9661 gcttggatca ggttgccgtt cgcatcgtag atgttcacgg tcgccgcgcc ggtgagtcga
     9721 atcgagatcg tgacgcgatc gggatcgggc ggcgtaacat cgcccggccg ctcggggtcg
     9781 ggcggggttt gctcgcccgg ccggtcgggg tcgggcgggg cttgttcgcc gggcgccaac
     9841 cgcgcgagcg aggtcccggc gcgatcgcgg agcgagacat tccacgatcc cgcgccgccc
     9901 ttcatcgtcg cgatggtcgt gctcgtgaag ccctgacagc tcatgtgcgg atcgccagaa
     9961 gccacgcggc ccaggagttg cccgctgccg tcgaggtact cggagatccg caagaggtcc
    10021 gataccggtc tgaactggag ctgaagtagc ggcggtagga tatccgggtt gtcgatcaga
    10081 tcagggttgt cgaccaggaa gggggcgacg aggtcggggt tatcgatcag ctgataggtg
    10141 acacccgtgg ttcgcccaac gttgttgagc gcgttgacct gaccttctgt caggccttgc
    10201 tcttgggttc caaggcgcga ggcgtcctcg tcgacacatc cgccgacagc gatcacgaca
    10261 aacatccatc ccttcatgcg ctctccttca tgttcaacag gcgatctggt tgcatcgagt
    10321 ggacgacatc gccgatcgcc gcgacgagtc gctggatatc gctggtcgta tggcagcgcg
    10381 tgatcgtcag gcgaatgccg cccgcatcgc gcggcaccgc agggaacgcg acggggttga
    10441 cgaggaagcc gaggtcgagc aggcgcgccg tcgcttcgat cgcgtcgtcg gcagtgccga
    10501 gcacgacgta ctggaccgga gtcggctcgc catcgatctt gacggcgcgt ggccccacga
    10561 tcggatgcga cgtgacgagt gcgcgcgcga acgccctgcg atcgcggtga gtggcttgca
    10621 gcgaaaagat ctcatcgccg agatgaatgc gcgcgctcgc acagatggcg ccgagcaccg
    10681 gaggcggcag ctggatgctg aacatgagcg aggggccgag gttctcgatc cgatgtttgg
    10741 tcgcgacgtc gggcacggcg aggactccgc cggcgcaacc aaatcctttg ctgagcgagg
    10801 tcgcgatcac ggtgcgctcg cgtggcagcg gcgcatcgat cagcgaaccg gcgccgtgcg
    10861 taccgcacca gctcatgcca tgcgcgtcat cgaggtacgc gtacagctgc gggtatcgcg
    10921 ccatgagctc ggacagcccg gcgatcggca agcggtcgcc gaacatgctg tagatgccgt
    10981 cggcgcagta ccagatgtgg tgcgcatcgt tcgcgactgc ttccttgatc gcggcctcga
    11041 gcgcatcgag atccgagtga gcgacgggca cgcgcgcccg ccgagcaccg agtgccgcga
    11101 cggcggtttg cacgctgtga tggacctgcc ggtcgagcaa tacgacgtcg cgtgcgccga
    11161 cgaggatcgg tagtgccgcg ccgtgcgcga gcgtcgtcga cccggcgagc acgacgggac
    11221 gcccgaaaat cgcagcgagt agctcctcgg cttcggcata cagcgcactc gtcacgaaac
    11281 agcgcgatgc actgaacgac acaccgaagc gatcgatggc ggccttcgcg cgctgcgcga
    11341 gtcgctcgtc gcgctcgagc ccgaggtaac tgcatgtcgc gaacgaaacg acgtcgcctt
    11401 cgggctcgag tcgaagggtc ctgccgttgc gatcgcgctc gcgcgcgacg cggtgggtca
    11461 cgcccgcggc tcgaccgcgc gccatcgttg cctcgagctc gggatgcatc gcctatccgt
    11521 aagacaggac gaacggagtc gactgtgcag acaaaacgcc ggctacggca cttcgtgctg
    11581 gtcgagccac tgcgccacgt cggcacgcgc acttgcggca cccgggccgg cttctgcata
    11641 gccagcttgc gcggccttcg cgaggctcgt tgcacgcgcg cgatctccgc cgggcaggga
    11701 ccacaaggtc cgcgccaacg cgaatcgggt gctcgcgaga tccggcgcac tcttgtcctt
    11761 gggctcctgg acgcgcagtg cgcgctcgag tgttttgacc gcgtcggtga gcgcgcccga
    11821 ggcaagctgc tgctggccga tcgcgctatc cgtccgcgct agcaagagct cgttgccgag
    11881 ctcctcatag atcgagcgcg cgcggcggta gagctcgagt gcctgcccgt gatgcccctg
    11941 cgcatcggcg gaggcggcga ggtagccggc atagcgagcg acgaggccgt gccgcggacc
    12001 gcgcagcttt tcggcgagcg cgagcgcgcg cgcgtggtgc cgttccgcct cctcgtagtt
    12061 gctctcgtgc tgcgcgacga gccctagcac tgcgctggca ctcgcaacga gcgcgtgttc
    12121 cggtccaagc gcagtttcga tcagcgcgag tgcacgctcg gcgtccacgc gcgcctcgct
    12181 gtaccgcgcg aggccgagct cggcgtcggc aacgttgaac agcgtatgcg cgtacagcgg
    12241 atggtgcgtg ccaagggtct gctcgtagat ggcgagggcc tgctggtaga gaggcaacgc
    12301 gtcggcgtgg cgctgccatt cgctgtagcc cacggcgagg ttcaccattg cgatcgcggt
    12361 ctgcggatga acgtcgccgt acagcgtgcg attcgcgtcg accacgcgct tcgctagctt
    12421 catcgactcc tcctcgcggc cttgctccca cgccgcgtcg gactgcatcg ctacgacctg
    12481 cgcttcgagc tccggatcag acccgagccc gctcagcagt gcctcggcgt cacgggcgta
    12541 ctcggcgccg agctcgggtt tgccgagttg gtggccgacc gtccagacca gctcggtacg
    12601 cgcgcgaacc gcgagcttga ggtcgcgccc ggcttcggcg gcccacactg cttcgtgcag
    12661 cgcatcggcg gcgtgtgcgt aatcggcgtt gatctcgagt gccttgccga gcaagagctg
    12721 tgcgtcggcc tcgagcggtt tgtagccgat ggccttcgct tccgcgacga gcttcgcgct
    12781 gcgctcggcc tgctcgcgat agcgaccgag gtcgcgtagc gctttggccg cggcgagctc
    12841 gacgcgcaag cgctccacct ggatacggac cgccgggttg cgcggcggcc gaaccggtgc
    12901 ggtcagtgct tcggcatcgg cgcactcgac gagcggggtc agcttgtacg cggctcgcgt
    12961 ggcctcgtcg agagtcgccg cgtcgggcgt catcagcagc gcgatcagcg ctcgcatctc
    13021 gtcgagacga tgatcgaggc attgcatacg gagccccatg agctcctcgc tctgctcgcg
    13081 agacacgcgc gtggccttgc aggcccgaac gcgcatatcg gtccaatcgc gtgcgtagcg
    13141 atcaaacgcc gcgctgactt tgccgaatgt cgcgtcggca aacggtcgcc ccgtcgcgcc
    13201 gaacgcaatg cgcatcgcct gctggcgcgg aacattccag acatcgtcga gtgcacgatc
    13261 tgctccctga cagaccagct gcgcgtcgct gtgcgaacgc cacatgaacg caccggccga
    13321 gaccgacacg gcgacgatgc ccaccgctgc gagtgcgcgc acgcgcagtc gagcgggatc
    13381 gcggccgagc gcggcgagta gctccgccat cgacgagaag cgagcctcgg gtcgggagct
    13441 gagcccgcgc accaggatcc ggtgaaccca ggccggtacg cgcgatgccc gcggtggcgg
    13501 ttgtactcgg tcgtcttcga tttgcttcgc gagctcctcg atcgagtcgc cggcaaacgg
    13561 tcgctgccga tagagcgtct cgtagagcgc gacgcagaag ctgaacagat cgctgcgggc
    13621 atcgagcggc tcgccgcgaa gctgctcgag tggcatgtat gccggcgtgc ctgcgatcgc
    13681 gcccgtgcgc gtcaacggat gcgtcggcag tgcacgtgca aggtcgcccg gtgcggccga
    13741 catcgacgcc gaggtcgccg ggtcgagaac gcgcgccaag ccaaagtcgg ttacgagcac
    13801 gcgcccatca cgcgcgatca gcacgttctc cggcttgaag tcgcgatgca cgaggccagc
    13861 cgcatgagca gcggcgaggc cgcggcccgc gcggctgaac acaccgacga tctcgcgcca
    13921 cgaacgcgag ggctcctcgc aatatcgcgt cagcgtcgaa ccatcgatga gctccatcac
    13981 gacgaagacc ttgccgtcct cctcgccgac gtcgtagacc gacacgacgt tctcgtgcga
    14041 gagcttcgcc attgcctgcg cctcgcgcag cgtgcggctg cggcgtgact cgccatcgtc
    14101 cgcgccttcc gtgcgcagca ctttcacggc gaccttgcga tcgagctcgg gatcgtaggc
    14161 ggcatagacg atgccattgc cgccggcacc gacgacatcg aggatcaagt accgcgctag
    14221 cgtcgaaccg cgcgggcgcg gttgctggct tgcaccagca ggcatgctat cggcgactgg
    14281 cagtgtcggc tggctcgcag cgacgagcgc acggcacacc gcacagtgat cgatgtgcgc
    14341 gtcgatcgcg gcgcgcgcgg tgtcgtcgag cttgccctcc gcgagctcga cgagcacatt
    14401 ctcatcgatg cacgaggcct ctggttcgct cgtgtcagcc atgggtggtc ttgagcagcg
    14461 agcgtagcgt gatgtcgagc cggctttgga ccaggtcgaa gaggctctcg cactcgctcg
    14521 ccgtgattga gagctgctgc atcaaaatgc ggcgggtctc gtcgagcatc tgcttgtgag
    14581 cggccgcgat ccaccgcatt gcggtcgtgc gatggacgcc gtagatgccc ccaattcgct
    14641 ccggcgtcag gccatcgagg tgctggaacc gcaacatgtt gcgttcgcgc tggggcagct
    14701 gcgagaacgc gtgcttgaac gcagcgacga acgcggcgcg atagcgcgcc ttgatgtaat
    14761 cgagctcggg atcgtcgccg ggctggagca tcgtgctcag atggtcgtcg tcgagcgcgt
    14821 cgtgccgacg ttcggatgcg atgaggttga gcgcggtgcg tagcccgacc atccggatcc
    14881 agctgtcgag cgcgccttgc cctcggtact gcgcgatctt tggtggagcc gcctgcgacg
    14941 acacgaataa tcggtcgcga accgcttgta gtgcttcatc cgtgaaccct gggtcggcgc
    15001 gcagacgcgc gatgtgcgga cgtaacttgt gcacgagctc cgtgtcgcag atgcgaaccg
    15061 cttcgggaca accattcgcg caagcgcagg cgaggtagag ctcctcggcg cgcacgcggg
    15121 cgacggcggt caccggatcg gtgtccgtag gtgcgtggtc gcccaggtgt ctgacaaacg
    15181 ctatcgattc gagcgcgacg ctcggccatc gctgtttgcc acggctgacc agctcctcga
    15241 gcccgctctg caatgcggtc ggaacgtccc ggacctcgcc tttccagcgt tctagaaaga
    15301 ccccaacgag ccgctccgcc accgcttcac ggtatcgcga gtttcgagag cgaaccaccc
    15361 ccggcgctcg tggtcgtctt catcgcctcg tcggtggcgg cggtcagctt ttcgctagac
    15421 aaggcgcacg aacaagcggc gaatccgcgg atgagcagcg cgaacggcgt cctcgattcg
    15481 atgcgtcgcc tcgacgatgt cggaggtcga tagatcgccc tgaaggcgga tgtctagatc
    15541 gacgtgaacc atatcgggac caatatgcat ggtgcgcggc agcgaggctt cagcgaccgc
    15601 aggatcatgc tgcgcgatct ggcgaacctt cgcgacgagc gtgtgctctg cggattcgcc
    15661 gacgagcagg ctgcggcact cgcgtgccag gatcacagcc acgacgacga gcaatacacc
    15721 gatgaccacc gaagcggcac cgtcgagctc ggggatgtcg agccagcgcg cgagcgtgat
    15781 cccgagcgcg gccaccgtga gcccggcgag cgcagccgaa tcttcgagca acacgacaaa
    15841 cgtcgtcggg tccttgctcc gcctgatccc ttcccacacg ccgagctcgc tgcacgcacc
    15901 cttgaagccg cgatagccaa ggagccagga cgtgccttcg aacacgaacg cgcccgcgag
    15961 aacgccataa ctgagaagcg tgctctcgag cgggcggggc gtcccgatgt gcaggatccc
    16021 ttgatagatc gagacaccgc cgccgacacc aaagatgctc atggcgacga ccagcgtcca
    16081 aaagtacgtc tcgagcccgt gcccgtaggg atgctcgggg ctcgcgggtc tacgagccaa
    16141 ccgcacgccg agcaggacca gtaacccgtt gccggtatcg accaacgagt gaattccttc
    16201 ggtgagcatc gccgagctgc cagagacgcc cgccgcgacg aatttcatcg tcgcgattgc
    16261 caggttcgcc gcgatcgctc cgtagatcgc caggagtcgg cggttcacga actgcctacg
    16321 tgcaacgcgc gtgcacgatc gcgtcagacg atttcttggc gttcactccg tgatcagcca
    16381 cgtcgcgatc tccgttcaag aacgaaggat gatcttcatg acctcgtcct gcttgtgcat
    16441 gaacatgtcg tagcccttcg gggcgtcgtc gagattcatg tagtgcgaga cgatgaagct
    16501 cgggtcgatg tctccacgct cgatgcgctc gagcaggggc ttcatgtagc ggtggacgtg
    16561 gcactgaccg gtcttgatcg tcaggccgcg gttcatcagc gcgccgagcg ggaacttgtc
    16621 cacgaacccg gcatacacgc cgaggatcgc gatgatgccg ccgctacggc aggccatgat
    16681 cgcctcgcgc acggccagcg ggcggtccat ctggatcttg accttctgct tcatggcgtc
    16741 atggacgtac tgcacaccgt gaccgtgcgc ttcgagtccg acagcctcga tgcaggcatc
    16801 ggggccgcga ccggcggtga ggtcgcgcaa cacttcctgg acgttctggt cctcgtagtt
    16861 gatcgcgtcg gtcgcgccgg ccttctcgat cgccatcttc agtcgatacg gatagcgatc
    16921 gatcgcgatc acgcgctcgg cgccgagcat cttcgcgctc gcgatcgcga actgaccgac
    16981 gggaccggca ccccacactg cgatcacttt gcgcggcgtg atctcgcaga actccgcgcc
    17041 catgtatccg gtcggaaaga tgtccgagag aaacagcact ttctcgtcgg gcaggtggcg
    17101 cgggaccttc aacggtccga agtcggcata gggcacgcgc acgtattccg cctggccgcc
    17161 cgagaaccca cccgtcaggt gcgaatagcc gaacgcaccg cacgtcggat ggcccatcag
    17221 cttctccggc atccagccgc tcgggtttcc gttctcacag cacgaaaaca ttccgagctc
    17281 gcactgctcg cagttgccgc acgcgatcgc aaacggcacg acgacgcggt cgccgacttt
    17341 gaggttcgtc acgcctctgc cgagctcgac gacctcgccc atgaactcgt ggccgaggat
    17401 gtcgcccttc tgcatcgtcg gcatgtagcc gttgtagagg tgcagatcgg atccgcagat
    17461 cgtcgtcgcg gtgacccgga tgatcgcgtc gcggttgttg atgatcttgg ggtcaggcac
    17521 gtgctcgacc gacacatgct tcttgcccat ccagcaggtc gctctcatgg ccgtgcgcct
    17581 ttctccgcgg tcggtcgacg cggttcgaag atcttcggtc ttctctgcac ctgctccggc
    17641 ggctgcgccg gatgcggcct cctgaattcc gtcgcatcgg agaacagaac ctcgccggtc
    17701 tcgacaagct gcttgaagcg gcggaggtcg cctttgatct gcggcttcgc gaagaacttc
    17761 gcgagcagcg tgctcggcgc cttcccggtg aaaccaagct gcatctcgac gcgaacctcc
    17821 gtcgagttgc ggctcggcgc ggtcgagaag atcacgctgc cgcgcgtctt gatcctcgaa
    17881 ccctccaccg actgccacgc gatccgctga tttggcatct cgtcggtaat ctcggcatcc
    17941 cattccaccg tgccgccgat cggaagcttg gcgacccagc gcgaacggac gcgatcgagc
    18001 tcttgcaccg atgcgaggta gtccatgaac tgcgggaacc tcgagaagtc gcggaacgcc
    18061 tggtagacct cctgcaccgg ccgattgatc gtgaccgagt agatcacggg gcgattggcc
    18121 gcctcgaagt ggcgctgcgt gccgcgcgcg gcgatcacgt cgagtgccat cacgccggcg
    18181 acactcgcca tcgcaccggc aaggcgcacg cggttcgtgc ggtgcgagct ggcggcgata
    18241 ccgaggagcc cgagatcgat cacgtcgcca gcgacgcgcg cccagagtgg caccggccgg
    18301 cgcggctcga gcaagaggcc gagtccggat gcgatctcgc ggattccgag cccgcgaagg
    18361 atcgtggacg cggcaatact cggctcgatc ccgatcagcc tcgagagcgt gcgtggaact
    18421 gcgagttgcg cggtaccgag cgcgaggctg aaccagccga gcccggcgga cagtttcgtc
    18481 gcgaaacgat catgctgatc ctcacgcgca gggcttcgaa aagcggagac agatttcacg
    18541 acgtcactcc ttcgctaggt ggcgaaccgt ctaagcctcg atcgcaggca gcaaacaacg
    18601 cgccactgcc gagcttttcc tgttgctcgg tctgcgccgt gcgtcgtgag atccaaacgt
    18661 ttgctgctcg aatcgagacg cacgtgtctc gttaacgctc actctgatcg cgagtagcac
    18721 cgacgcgcgc gaaccgactt acttcttcat cagcccagcc ttcaggacct tcgggtccgg
    18781 cagcaggatc acgaagtcca gccggagccg ccactcgggc ccaccatcgg ggcttgcggc
    18841 gaaccagccg gcaccgacct gcacgctgag cggcaagaag ccgatcttgg tcaatcgggc
    18901 gccgaccagc tcgagcgggg cagtcacctt gtcatcgttg gcttcccagt tgagggtggt
    18961 ctcggtctcc gcggtgaggg tccacttgtg cttcgtgtac gagacgaacg gctggatgaa
    19021 catctgactc acgtcgggag cgttgacgtc actggtatcc gcgaccgacc acagctggtt
    19081 cacgaggagg ccaagcgtcc acggtgaatc gatgtacagc gcgacgcctg tggggcccat
    19141 caaccacttg cgtcttgttc acaagtagct gcgtgttacg ccgtagacgg actagaagca
    19201 ggtcacaaag ttgtcgaacc gcgcgacgcc agacgtgagc gccgcggtgt acgagcccga
    19261 cgcgagctcg accgtgagcg cggtcatggg aatcgagacc ggcaccgtac cgagctcggt
    19321 ccactcgccg aggctgttgc gcacagacat gcggatcaga ttcgcctggc gatcgtgagc
    19381 caacttgaac cgacgatgct tgtccggttc gtaggggatc tcgatcgtgc ggtccgatcc
    19441 atcgacggag tagccgtacg acagctcgcc gtgatccacc gcgatcgagt accagtcgtg
    19501 tccgcgatcc cactccatgt atgtctcgct cgaggtgtcg ggtaccgtga ccacgtcaac
    19561 ctcgatcgac gcgcccgtga agtcgcgaac cgcgctgtcg attccgccgt gatcgtccgc
    19621 ggccttgttc agcgagatcg agagtgttcc gtcgatcgcg acgactgcgg tcggcggatt
    19681 ctcgaacatc gtccactgtg tgccgagcat cgcaaagtcg tcggccacgc agttcgtcgt
    19741 gtgcatgtct tgcgtcgcgt cgaccgagtc tgcagaaccg ccggcatccg tatgccccgt
    19801 caggtcgtcg atctggaaca gcaagtcaca ccctgcgagc aagaccatgg ggaccgctac
    19861 aagtacggct ctgggcattg ccggccctgg attgcacgcg ctgtgcagaa gtcaggtctt
    19921 cgaaaccaat gaacccgaat ccatcggcct gactactcgg cgatgcatca ttggatacca
    19981 ccggacgcac gccgccgcgt cacacccagc cgggcagaag gccgtcgacg agtcggtcag
    20041 gccgcaagac gccttcgtct gcgaagaatc gatcggcttc ggcgcggtgc gcgtcgccgg
    20101 ccgagccgcc gatgatcgcg gcgaggcgcc agcggcaggc tgctgccaag gcacgctggc
    20161 caccagcacc gaggacttcg acgagggcgg tgaatcgcgc ggctgcgtcg tcgagatcgc
    20221 cggcgacgag cgccgcggag gcggcgacca agtcggccaa atgggtgcca gcctgtagcc
    20281 cgatgcgggt gcgcgcgatc caacggcgtg ccttggcgag atagcgcttg cacccggctg
    20341 cgtcgccccg cgcgcgcgcg agcgaggcgc gcccgacctc gagctgggcg gcgaactgcg
    20401 tgcactcgag ccggatcatc gggatctgat agagcagcga cgcacgcgcg cgggcccagt
    20461 cggcgtcgag cgtcggatcg ctgtcggggt tgcgctggta gagcgcgatg agagtgcggt
    20521 tcttgagcgc gaagaagaat gggttcgtga cgtcgtcgcc gatgccttcc gtgtgccagc
    20581 cggcaagcgc atcgcggaga tccgcgagcg cttcgtccgg acggtcgacg agcaggtgga
    20641 gtatcgcaaa gaacgtgcgg tgcgaaactt cgacgaaccg gttaccgatg cgttgcgcgc
    20701 tgcggatcgt caccggcacg cggcggcgca gctccttgag ctcgcccagg atgctgagag
    20761 accacacgtg atgttgctcg atgatgtcgc cttcgaaggt gtgactacgg cgatcgggga
    20821 gcaacgacgc ggcgcgctcg cacgcggcgg ctgcgccttg ccagtcgttg atcaggtaga
    20881 acaggtacgc cacgtccgcg aacgccgcgt acggcgcggc gctgggatcg tcgctttcgg
    20941 cggcggaacg atgcgtggcg tcgcgcaggc gctcggccag ccggcgacga cccatgcccg
    21001 acgcggcgac cgcggccgcg cacatcgctc gtgcgatccg ggtcggatcg ccggccggca
    21061 tcgcaagcaa cggcaggcgc atcgcggagc acgcgccgcg cgtggagtcg accatcgaga
    21121 ggccggtcgc gagcgaccac gagacgtcga tgcggatccg gttgcgctcc gtgacctcct
    21181 cgggtgagcg gtgcggccaa cgcaggcgtg agaaccggag ccgggtctgg taccagatca
    21241 agcgcgtgat cgcgctcgcg gttgaccccg gcagtgagag atcgatctcg ttggcgagct
    21301 cgatggccag ctcgcgaccg cgcgccagat gaccaccgat caagagctgc tcggccgcgc
    21361 gccggcgcag ctcgcgcgct gcatcgatcg attcgccttc cgccgcactg aggaaggcgt
    21421 ccgctgcttc ggccgtgcgc ccggcgcggg ccaagacgtc ggcgcgctcg gcgatcagcg
    21481 cgcggcggcg atcgggcgtt agctcgccca gctcgagcgt gatgcgcatc cactcggcgg
    21541 ctcgatcgaa tgcgagtgcg tcgagtgcgt gctgcgccgc aacctcggcg agcgcaccgg
    21601 cgcgagcgcc ttcgccggcc tgggcgaggt ggtaggtgag cacctcgacg ccgccgcccg
    21661 ccgcctcgag gctgctgacg atcgcgcgat ggatcgactg gcgccgttcg gcgggcacgc
    21721 gtgcggcgac cgtctcgcgc acgcgatcgt gatacggctc gacgagcgac gcgaagccac
    21781 gcgccgcgcg cacgctaccg tcatcgctgt cgtggtcacg tccgccgatg attcggacca
    21841 agcgcgcagc acgcagcgcg ccgagatcgc gagcgacgtc gtcggcagcg agtccggcgg
    21901 cggccgacac gacaccgagc ggcagcgctg ccgcgccaag cgttacgact tcgagcaatg
    21961 cgcgtgcacc cgcgtcgagc tcaccgatgc gttgccagag cgcatcgtcg agccgcaggt
    22021 tgagcaaacc actcgtttcg ctgcggcggg cgagctcggt gaggaacatc gggtgtccgc
    22081 cagcgtcgtt cgcgagccga gcagcgtcgg cgtcgggaga tgtcgcgcgg accagcgcct
    22141 cggcctcgtc gcgagccagg cctccgagct ggatcacctc gacagccacg cgcatcgcgt
    22201 tgcgcgcagc gatcacgcca tcgtcgctga ctcgcgccgt cgcaacgaga agcaccggtg
    22261 gcgcgccggc gcccgcgaac agctcgccga gcaacgcgag gctatccgcg tcggcccaat
    22321 ggatgtcgtc gagcatgagg acgacagggc ggcgtgcagc gacagcggcc aacagatcgg
    22381 cgagtgccgc aaacgcgagc gagcgctgat cgatctcggc agcgcccgga tcgcgcgcgc
    22441 gatcgagccg agcggcgaca tcgagcgccg ccacacctcg cagcaccggg aagatctgga
    22501 gcag
//
LOCUS       DASUFX010000010         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1112835, whole genome shotgun sequence.
ACCESSION   DASUFX010000010 DASUFX010000000
VERSION     DASUFX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112835"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFV99_00470"
     CDS             <1..663
                     /locus_tag="VFV99_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057792.1"
                     /translation="AVGGDERILGLSSVAARGQLPAPAGPPCNCDPSTFFDVGAAVAA
                     AKQATNGASSWTHIGIQDLHLTGGNYYVTAADVVGLTAIHVDAPSSVFVDGSLRQVGA
                     ELWKLAPGATLDLFVSGNVASVGALVAGNPADPTAFRLYVGGSGDTVVGSVGAALFFG
                     SIYAPRSAIAYVGDAQIVGSIFAKTIKGVGVLTLEYRDPIAPPTSCDPPTGGDPDVPT
                     FL"
     gene            696..887
                     /locus_tag="VFV99_00475"
     CDS             696..887
                     /locus_tag="VFV99_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057793.1"
                     /translation="MCKRGRILAIKWGMNPNSSSLGVDVTFLLVGATALAIVTPVLGA
                     LLRWRKPVPAESPKPSTET"
     gene            931..1788
                     /locus_tag="VFV99_00480"
     CDS             931..1788
                     /locus_tag="VFV99_00480"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX5057794.1"
                     /translation="MSRTSAISAEAERAAHEAAPWIARLARLGFLAHALLYITVGALA
                     TSAALRLLGTRAPVPSKTVGSRGAMAELLQAPAGHVLLYVLAVGLAGYGIWRAIAAII
                     DPEHHGHDAKGIVHRLGSAGVAIIHFGLAYSAVRIAMGHLGAAHEGTEERHWTARALA
                     TPGGAYVLFAIALGLAAYGVYEIYCAARAKLGEGLTLSMSSRARRWVLGISRFGIAAR
                     AIVFVVTGGLVAAAVHERNPEKAPGPGRSLRELFELGTIPFLIVAVGLIAFGVYQLLN
                     AKYRQIRVA"
     gene            1804..3087
                     /locus_tag="VFV99_00485"
     CDS             1804..3087
                     /locus_tag="VFV99_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5057795.1"
                     /translation="MGLLTRARDAVGTIADRLRAVRQHPPRRARFERFGAIVQLTIPR
                     ALVFVDRAMARRVVAVPSEPAAWREPESELGEHVLSAPLEAHLQITNRCAAGCQGCYT
                     GASAEGAPNEWGLAEWKRALDALADAGVFHVALGGGESATLPWLGELADHARARGIIP
                     NLTTSGLDGLDRLLAIADRFGQINVSLDGLGDTYAAVRGFDGFARADAAIRALRAIKH
                     EVGINVVVTRHNFAELDTIFAYAAERRLSEVELLRFKPSGRGARAYSDLRCTDAQHRA
                     FLPTILEAARRHRVRVKVDCSYTPMLAHHRPDRALLAELAVYGCTGGDFLVGAKPDGR
                     LTACSFASPPPALDGQRPRVDALATYWTAPDAFGAFRNWRAAREPCASCEYHSLCRGG
                     CKVVSAHLAGDLAAPDPECPRVIDEGARRVHLNVL"
     gene            3097..>3318
                     /locus_tag="VFV99_00490"
     CDS             3097..>3318
                     /locus_tag="VFV99_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057796.1"
                     /translation="MDDPYRFSQQTGTCPRCQNETANDGELNRLVCVRGCGEWYPKDK
                     LDAVFAWHELDKRAGGLGLDGQHAQPTNWP"
BASE COUNT          485 a         1157 c         1092 g          584 t
ORIGIN      
        1 gccgtcggtg gcgacgagcg aatcctcggc ctgtcgagcg tcgcagcgcg cggccagctc
       61 cctgcgcccg ccggaccacc gtgcaactgt gatccatcga cgttcttcga cgtcggagca
      121 gccgtcgccg ccgcgaagca agccacgaac ggcgcgtcga gctggaccca catcggaatc
      181 caggaccttc acctcaccgg cggcaactat tacgtcacgg cggcagatgt cgtgggcctc
      241 accgcgatcc acgtcgacgc gccgtcgtcg gtattcgtcg acggctcgct tcgtcaggtc
      301 ggtgccgagc tgtggaagct cgcaccgggt gcgaccctcg atctcttcgt ctccgggaat
      361 gtcgcatcgg tcggcgcgct cgtggccggc aaccccgccg atccaaccgc gttccgcctc
      421 tacgtcggtg gctccggtga caccgtcgtc ggttcggttg gcgctgcgct gttcttcggc
      481 tcgatctacg cgccgcgctc ggcgatcgcc tacgtgggcg acgcgcagat cgtcggctcg
      541 atcttcgcga agacgatcaa gggcgtgggc gtgctgacgc tcgagtaccg cgatccgatc
      601 gcgccgccga cgtcatgcga cccgcccacg ggtggcgacc ctgacgtgcc gacgttcctc
      661 tgatcgtcgt cgagcccgcg tgctagattg cggtcgtgtg caagcgcggc cgcattctcg
      721 cgatcaagtg gggcatgaac cccaactcgt cgagtctcgg cgtcgatgtc acgttcttgc
      781 tcgtcggcgc gaccgcgctc gcgatcgtca cgccggtgct cggtgcgcta ctccgctggc
      841 gcaagccagt gcctgccgag tcgccaaagc cgtcgaccga gacataagcc gagacgctcg
      901 gctgcgctgg tatttgcatt acctgtcagc atgagccgca cgtcggcgat ctctgcagaa
      961 gcggaacgtg ccgcacacga ggctgcgccg tggatcgcgc ggttagcccg cttgggattc
     1021 ctcgcacacg cgctgctcta catcaccgtc ggtgccctgg cgacgagtgc cgcgctgaga
     1081 ctactcggca cgcgcgcgcc agtgcccagc aaaacggttg gctcgcgcgg cgcgatggcc
     1141 gagttgctgc aagctcccgc aggtcacgtg ctgttgtacg tgctcgcggt cggcctcgcc
     1201 ggctacggga tatggcgcgc gatcgccgcg atcatcgatc ccgaacacca tggacacgac
     1261 gcgaagggca tcgtgcatcg cctcggctcc gctggtgttg ccatcattca tttcggcctc
     1321 gcctattcgg ccgtgcgcat cgccatggga cacctcgggg cagcgcacga aggcaccgag
     1381 gagcggcact ggacagcacg cgctctcgcg acgccgggcg gcgcatacgt gctgtttgcg
     1441 atcgcgctcg gactggccgc gtacggcgtc tatgagatct actgcgccgc gcgcgcgaag
     1501 ctcggcgagg ggctgacgct gagcatgtcg tctcgcgcac gccggtgggt cctcggcatc
     1561 agccggttcg gcatcgcggc gcgcgcaatc gtcttcgtcg tcaccggcgg gcttgtcgcg
     1621 gcggcggtgc acgaacgcaa cccagagaaa gccccaggtc ccgggaggtc gctgcgcgag
     1681 ctgttcgagc tcggcacgat cccgttcttg atcgttgctg tcggcctgat cgcgttcggc
     1741 gtgtatcagc tcctcaacgc gaagtaccgg cagatccgcg ttgcctaggc tacagtccgc
     1801 ggcgtggggc tgttaacgcg tgcgcgcgat gccgtcggaa cgatcgcgga tcggctgcgt
     1861 gctgtgcgcc agcatccacc gcgtcgcgct cgcttcgaac ggttcggcgc gatcgtgcag
     1921 ctgacgatcc cgcgcgcgct ggtgttcgtc gatcgcgcga tggcacgtcg cgtagtcgcc
     1981 gtgccgagcg aacctgccgc gtggcgcgag cccgagagcg agctcggcga gcacgtcctc
     2041 tccgcgccac tcgaagcaca cctccagatc acgaaccggt gcgccgccgg ctgccaaggc
     2101 tgctacaccg gcgcatccgc cgagggcgcg ccgaacgaat ggggcctcgc cgaatggaag
     2161 cgcgcgctcg atgcgctcgc ggacgccggt gtgtttcacg tcgcactggg cggcggcgag
     2221 agcgcgacgc tgccatggct cggcgagctc gccgaccacg cacgcgcgcg cggcatcatt
     2281 ccgaacctga cgacgtcggg cctcgatggt ctcgaccgtt tgctcgcgat cgcggatcga
     2341 ttcggccaga tcaatgtctc gctcgatgga ctcggcgata cgtatgccgc cgtgcgcggc
     2401 ttcgacggct tcgcgcgcgc cgacgccgcg attcgtgcgc tgcgcgcgat caagcacgag
     2461 gtcggcatca acgtcgtcgt cactcgccac aacttcgccg agctcgacac gatcttcgcg
     2521 tacgcagccg agcgccggct ctccgaagtc gagctgctcc gcttcaagcc gtcgggccgc
     2581 ggcgcccgcg cctactccga tctccgctgc accgatgcgc agcatcgcgc gttcttaccc
     2641 acgatcctcg aagcggcgcg ccgtcaccgt gtgcgcgtca aggtcgattg ctcgtacacg
     2701 ccgatgctcg cgcatcatcg tcctgatcgc gcgctgctcg ccgagcttgc ggtgtatggc
     2761 tgtaccggcg gcgatttcct cgtcggtgcc aaacctgacg gtcgcctcac tgcgtgcagc
     2821 ttcgcgtcgc cgccaccggc gctagatggt cagcgcccgc gcgtcgatgc gctcgccacg
     2881 tactggaccg cccccgatgc attcggtgcg tttcgcaact ggcgcgctgc tcgcgaacca
     2941 tgcgcgtcgt gcgagtacca ttcgctgtgt cgcggcggct gcaaagtggt gagcgcgcat
     3001 ctcgctggag accttgccgc tccggatccg gaatgcccgc gcgtgatcga tgagggtgcc
     3061 cggcgggtcc acttgaacgt gctatagccg caccgaatgg acgatccgta tcggttcagc
     3121 cagcagaccg gcacctgtcc gcgctgtcag aacgagaccg cgaacgacgg tgagctgaat
     3181 cggcttgtct gcgtccgtgg ctgcggtgag tggtatccga aggacaagct cgacgcagtc
     3241 tttgcctggc acgagctcga caagcgcgcg ggaggcctag gcctcgatgg ccagcatgcc
     3301 cagcccacga actggccc
//
LOCUS       DASUFX010000011         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1391044, whole genome shotgun sequence.
ACCESSION   DASUFX010000011 DASUFX010000000
VERSION     DASUFX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391044"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3333
                     /locus_tag="VFV99_00495"
     CDS             <1..3333
                     /locus_tag="VFV99_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5057797.1"
                     /translation="INTMVDQLSSFASEVTRVAREVGTEGRLGGQADVRGVSGVWKDL
                     TDNVNFMAGNLTSQVRGIAKVVTAVANGDLKQKLTVEAKGEIAALAETINSMTETLAT
                     FADQVTTVAREVGVEGKLGGQAKVPGAAGIWKGLTENVNELAANLTTQVRAIANVATA
                     VTQGDLTRSITVETQGEVAALKDTINEMIRNLKDTTQKNTEQDWLKTNLAKFSRMLQG
                     QRDLPAVGEMILSELAPVVRAHQAEFYVLTSDRDQQRLVLLASYASSGRRAHGKEVDL
                     GEGVVGQCAIEKRKMLLTNIEARRFRISTGLTRSQPAEVLVMPIVFEGELRGIVELAS
                     LDSFSPSHQAFLDQLMESIGIVIHTIEANMRTETLLTQSQSLAEELQQTNQALEEKAR
                     QLAHQNQEVERKNSEVEQARQALEEKATQLALTSKYKSEFLANMSHELRTPLNSLLLL
                     SDKLAKNPEGNLSLRQVESAKTIHASGNDLLALINDILDLSKIESGTVVADVSELRLL
                     DLQRYVERTFRHVAEAKQVGFEVHLDPHVPRTMLTDIKRMQQIIKNLLSNAFKFTHEG
                     SVSLLVGPAAGGWSPDNQDLVEADQVIAFSVVDTGIGIPPDKQQIIFEAFQQADGSTS
                     RKYGGTGLGLAISRELSKLLGGEIRLSSAPGEGSTFTLYLPVEAPRSTRATRPGTVEA
                     RPALAGAPKAPVQPLYVEDETPTSELNEAGDDRASIHPGDRTMLIVENDLAFAKVLLD
                     AARRRGFKGLVSATAAAAISLIKEYMPTIVSLDIFLPDMPGWRILDRLKADLSTRHIP
                     ICIVSTDDSRDRALSSGAIGFITKPVASTDVIDDAIAQLADYVARPRKRVCLAMSPGG
                     DLDVVQARFADSAVDVVATTEAAEICDLLRKDQIDCLVIESGSTDLQPADLIEAVEQR
                     VLTRQLPIVVYGSGGGDALSRWRRRDGMFALREARSLERLMDAVYFSLHRSMRELSEG
                     EREAIEGLHHDSRVLEGKKALIVDDDMRNIFALSTVLHDHGMEIISANNGREAIRFVQ
                     EDPSIDIVLMDIMMPEMDGMTTMREIRKLEQRRDLPIIAVTAKAMKGDREKCIEAGAW
                     DYLSKPVDTIHLLGVLKSWLHR"
     gene            3368..>4160
                     /locus_tag="VFV99_00500"
     CDS             3368..>4160
                     /locus_tag="VFV99_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5057798.1"
                     /translation="MEEKVNILVVDDLADKRLAFREVLDDLDENLVLVGSGAEALREL
                     LEREFAVIVLDVNMPDIDGIETAELIRKHGRTAHTPIIFVTAYADEMQTERGYELGAV
                     DYILTPVVPAILRSKVRVFVDLYRAQRRAAKVARAEAARAAAEAAQRRSDLIALASRE
                     LSSSLDLRASMERLLEVLVPSVGGCAVLSVDAGNGHLVLYRQDREPARMLEALTLLPP
                     GVRRAMAEVQSGGYPLEIRTDAGGSIHWPPWLRVAHVLPLRVGERN"
BASE COUNT          777 a         1333 c         1367 g          683 t
ORIGIN      
        1 atcaacacga tggtcgatca gctctcgtcg ttcgcgtcgg aagtgacgcg cgtcgcgcgc
       61 gaggtaggca cggaaggccg cctcggcgga caggccgacg tacgcggcgt gtcgggcgtg
      121 tggaaggacc tgaccgacaa cgtgaacttc atggccggca acctcacgtc gcaggtgcgc
      181 ggtatcgcga aggtcgtgac ggccgtcgcg aacggcgacc tgaagcaaaa gctgaccgtc
      241 gaagcgaagg gtgagatcgc ggcgctcgcc gagacgatca acagcatgac ggagacactc
      301 gcgacgttcg ccgaccaggt gacgaccgtg gcgcgcgagg tcggcgtcga aggcaagctc
      361 ggcggccagg cgaaagtgcc gggcgccgcc ggcatctgga aaggcctcac cgagaacgtc
      421 aacgagctcg cggccaacct gacgacgcag gtgcgagcga tcgcgaacgt cgccaccgcc
      481 gtcacgcagg gcgacttgac tcgttcgatc acggtcgaga cgcaaggcga ggtcgcggcc
      541 ctcaaggaca cgatcaacga gatgattcgt aacctgaagg atacgacgca gaagaacacg
      601 gagcaggact ggctcaagac gaacctcgcg aagttcagcc gtatgttgca aggccagcgc
      661 gacctgcctg ccgtgggcga gatgatcctc tcggagctcg ctcccgtcgt gcgcgcccat
      721 caagctgagt tttacgtgct gacaagcgat agggatcagc agcgcctagt gctgctcgcg
      781 agctatgcct cctcgggccg tcgcgcgcat ggcaaggaag tagatctcgg cgagggcgtc
      841 gtcggtcagt gcgcgatcga gaagcgcaag atgctgctca cgaacatcga agcgcgtcgc
      901 tttaggatca gcaccggcct gacgcgctcg caaccggcgg aagtgctcgt catgccaatc
      961 gtcttcgagg gcgagctgcg cggcatcgtc gagctagcat cgctcgacag cttcagcccg
     1021 tcgcatcaag cgttcctcga tcagctcatg gaatcgatcg gcatcgtcat tcacacgatc
     1081 gaagccaaca tgaggaccga aacgctgctc acgcagtcgc aatccctcgc ggaggagctg
     1141 cagcagacga accaggcgct cgaggaaaag gcgcgccagc tcgcgcacca gaaccaggag
     1201 gtcgagcgca agaacagcga agtcgagcag gcccgccaag cgctcgagga gaaagcgacg
     1261 cagctcgcgc tgacctcgaa gtacaaatcc gagttcctcg cgaacatgtc gcacgagttg
     1321 cgcacgccgc tcaacagctt gctgctcctg tcggacaagc tcgctaagaa cccggaaggc
     1381 aatctcagtc tgcgccaggt cgaatccgcc aagacgattc acgcgtccgg caacgacctg
     1441 ctcgccctga tcaacgacat cctcgacctg tcgaagatcg agtcgggaac ggtcgtcgcg
     1501 gacgtcagcg agctgcggct gctcgatctg cagcgttacg tggagcgcac gttccgtcac
     1561 gtggccgaag cgaagcaagt cggcttcgag gtgcatctcg atccgcacgt gccgcgcacg
     1621 atgctgaccg acatcaagcg gatgcagcag atcatcaaga acctgctgtc gaacgcgttc
     1681 aagttcaccc acgaaggctc ggtgtcgctg ttggtcggcc ccgccgcggg cggctggtcg
     1741 cccgacaatc aggatctcgt cgaggcggac caggtcatcg cgttctccgt cgtcgatacg
     1801 ggtatcggca tcccgccgga caagcagcag atcatcttcg aagcatttca gcaggccgac
     1861 gggtcgacgt cccgtaagta cggcggcacg ggccttgggc ttgcgatcag ccgcgagctg
     1921 tcgaagctgc tcggcggcga aatccggctt tcgagcgctc ccggcgaggg cagcacgttt
     1981 acgctgtacc tgcccgtcga agcgccgcgt tcgacgcgcg cgacgcggcc gggcaccgtc
     2041 gaggctcgcc ctgcgctcgc cggggcgccg aaggctccag tgcaaccgct gtacgtcgag
     2101 gacgagacgc ctacctcgga gctcaacgag gcgggtgacg atcgcgcgag cattcatccg
     2161 ggcgaccgca cgatgctgat cgtcgagaac gacctcgcgt tcgcgaaggt gctgctcgat
     2221 gccgcgcggc gccggggctt caagggcctc gtcagcgcta cggccgcggc tgcgatctcg
     2281 cttatcaagg agtacatgcc gacgatcgtg tcgctcgaca tcttccttcc cgacatgccg
     2341 ggctggcgca tcctcgatcg gctgaaggcc gatctgtcga cgcggcacat cccgatctgc
     2401 atcgtctcga cggacgactc ccgcgatcgc gcgttgtctt cgggcgccat cggtttcatc
     2461 acgaagccgg ttgcctcgac ggatgtcatc gacgacgcca tcgcgcagct cgccgactat
     2521 gtcgcgcggc cgcgaaagcg tgtctgtctc gcgatgtcgc cgggcggcga cctagacgtc
     2581 gtgcaagcgc gcttcgcgga ttccgccgtc gacgtcgttg caacgaccga agccgccgag
     2641 atttgcgact tgctgcgcaa ggatcaaatc gactgcctcg tcatcgagtc cggctcgacg
     2701 gacctacagc ccgccgatct catcgaggcg gtcgagcaac gcgttctgac gcgccagctg
     2761 ccgatcgtgg tctacggctc cggcggcggg gatgctctgt cgcgctggcg gcgccgcgac
     2821 ggcatgttcg ctctgcgcga ggcgcgctcc ctcgagcggc tcatggatgc cgtgtatttc
     2881 tcgctgcacc gctcgatgcg cgagctgtcc gagggcgagc gcgaagccat cgagggtttg
     2941 caccacgatt cgcgcgtgct ggaaggcaaa aaggcgctca tcgtcgatga cgacatgcgc
     3001 aacatctttg ctctttccac ggtgttgcat gaccacggca tggagatcat ctcggccaac
     3061 aacggccgcg aggcgattcg attcgtgcag gaggatccgt ccatcgacat cgtgctgatg
     3121 gacattatga tgccggagat ggacggtatg acgacgatgc gcgagatccg caagctcgag
     3181 cagcgcaggg acctgccgat catcgccgtc accgcgaagg ccatgaaggg cgatcgcgag
     3241 aagtgcatcg aggcaggcgc ttgggactat ctctcgaagc cggtggacac gatccacttg
     3301 cttggcgtgt tgaagagctg gctgcaccgc taacacgggc tactgacact accgccaacc
     3361 gctgtccatg gaagagaaag tcaacattct cgtcgtcgac gacctggccg ataaacggct
     3421 ggcgttccgc gaggtgctcg acgatctcga cgagaacctc gtgctcgtcg gttccggcgc
     3481 tgaggcgctg cgcgagctgc tcgagcgcga gttcgccgtc atcgtgctcg acgtcaacat
     3541 gcccgacatc gatggcatcg agaccgcgga gctcatccgc aagcacgggc gcacggcgca
     3601 tacgccgatc attttcgtga ccgcgtatgc cgacgagatg cagacggagc gaggttacga
     3661 gctcggcgcc gtggactaca tcttgacgcc cgtcgttccg gcgatcctgc gcagcaaggt
     3721 gcgcgtattc gtcgacctgt accgcgcgca gcggcgcgcg gcgaaggttg cgcgcgccga
     3781 agcggcgcgc gcggccgccg aagcggcgca gcggcgctcc gacctgatcg ctctcgccag
     3841 ccgggagctg tcgtcgtcac tcgatcttcg cgcgagcatg gagcggctgc tcgaggtgct
     3901 cgtgccgagc gtcggcggct gcgcggtgtt gtccgtcgac gccggcaacg ggcatcttgt
     3961 gttgtaccgg caggaccgcg agccggcgcg catgctggag gcactgacgc tgttgccgcc
     4021 gggcgtccga cgggcgatgg ccgaggtgca gagcggaggc tatccgctcg agattcgcac
     4081 cgacgcgggc gggtccattc actggccgcc gtggctgcgc gtcgcacacg tgttacccct
     4141 ccgagtcggc gagcgcaaca
//
LOCUS       DASUFX010000012         6750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_1877,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000012 DASUFX010000000
VERSION     DASUFX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VFV99_00505"
     CDS             <1..1563
                     /locus_tag="VFV99_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5057799.1"
                     /translation="ESTGHLLTIKEVVDGVDTTHEMPRIFMLDMRQPIGHGFRERRMI
                     NIPDPDALFIIENDDTTVPPDRLALPRVIYDHLRGHPFACGPLLGSRGQPVGALGLSS
                     YQGRQPIPDELFEHGLLHAFMNHLGIAMERAIHTARLERLNAELLEAHAAIASSARIK
                     AVGELAAAVAHDLNNLSGIALLAVSAGRHSPKAAFDVLPRIERANRTIGELVGRLQRV
                     ARQAHPEDGTGDVACVHQIVEDILAMISPLLRERAITVDLDLQPTPAAHADPSFVHQV
                     VLNLILNAQDALESITGRPRKLRVLVRGDGDMVRLEVTDNGPGIAAEVLSRLFEPFVT
                     TKHGAHAGLGLAAAAGSLKHFGGSIEGRNVPGGGASFEVMIPAASTRSERPLAQGEPP
                     QPAVQSRRGAILAIDDDEDILAVVRVYLDSLGYEVHTTTDAEQALQLVPGRSFDVVLC
                     DVGMPKQNGLDVCQLLREAGYRGKIVLMTGWDTQRVNVDRRSQSSDAVLKKPFLGSEL
                     IDLIDAMLAPPN"
     gene            complement(1560..2594)
                     /locus_tag="VFV99_00510"
     CDS             complement(1560..2594)
                     /locus_tag="VFV99_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX5057800.1"
                     /translation="MVARLLCLTILIGCGGKYGGTPKTTPTPTAKGGIEKAALPYSVL
                     DRSGHQVDTAAFWAQVGAARVVCVGEDHPNPHHHWFQLETFKHVSQHKPLALGMEMFQ
                     RPFQGVLDDYAAKRIDEAALLSRSGYEERWSYDWGFYQPTVNAAVAVGATLIALNTAR
                     ELTKKVVRQGLASLTPDEKKQIPELVLDDKAHRAWFDDTMNEMGGAGAHSAPKKDDDE
                     AKKDAKKDEKKDDKKEEPSDAPKMPSADDIYTAQVLWDETMADGAAKWAKATPQGLLV
                     LLAGNGHCHDTAIVNRIKRRGVDKVISLRPVLDVEGRVAEALAKPMNDYIIVLQLPPD
                     AKAAMEKADN"
     gene            2618..3637
                     /locus_tag="VFV99_00515"
     CDS             2618..3637
                     /locus_tag="VFV99_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX5057801.1"
                     /translation="MELPYLTAALPGIGGVLRASPEDFVVDEEPAYSPSGSGDHVFVR
                     IEKRGLTTAMAVERIARALGVNARDIGVAGMKDRHAVTRQWLSLPPPVTPEAASALTL
                     DDVTILEATRHGHKLRTGHVRANRFVLRVRDVAADAEDKARAILDALAARPGAPNWYG
                     EQRFGRDGDNAERGLAIVRGEKPPRDRKLARLLVSALQSKLFNEWLTARLADGLYARV
                     VAGDILHKTGGGQFVCEDPSVDQPRLLAGELCITGPMFGDRMRAPAGEAQEREQRILA
                     AAGLSADAFASVRAIAEGTRRDATVEVAAAAVRTVEPGVVEVAFGLPGGAYATTVMRE
                     LMKSS"
     gene            3647..4300
                     /locus_tag="VFV99_00520"
     CDS             3647..4300
                     /locus_tag="VFV99_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5057802.1"
                     /translation="MLSEQFVAFAQLGAEWVLWLLIGMSVLSVGIMIDRALWFRRRDT
                     DTAKFTRELRGAFERNELDRIATKYKDDPAVPVQVALRGLAERSQGPEAVAEAMQGER
                     VRWRNAGDRHLIVLGTLGNNVPFVGLFGTVLGVINAFENLRQKTAQAEDRTLDLIAEA
                     LAATAFGLLVAIPAVIAFNYFSRRMRVLLGSADEAAHTVMSMVHGEHHTKEAANGGK"
     gene            4287..4700
                     /locus_tag="VFV99_00525"
     CDS             4287..4700
                     /locus_tag="VFV99_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5057803.1"
                     /translation="MAGSSLYQDSDDPGGITDINVTPFVDVVLVLLVVFIVTAKLIVA
                     RGVDIEKPKASSGGDVQSTLRIAVKADGTLFVNGDEYKDDTAALSRIKEIAATTSKPK
                     AIIAGDRKTAYGGVMRAIDLATQAGVPIALENERP"
     gene            4792..5577
                     /locus_tag="VFV99_00530"
     CDS             4792..5577
                     /locus_tag="VFV99_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5057804.1"
                     /translation="MRLDSPFVLATAGTLAIHLLLAVAGDAIVVLYPHKQYEEAPRVE
                     LIDIEVPPVVKPPPPPVQPERPEPVKETRPVPQTVQKVVRQQPTAPPPPENEPPPPVD
                     TPPSGGAPLATMEGVIPSATGVPVAVGKRTSEHVGRGGTGTGTGAGSGTGSSDEPPKP
                     VSVATIKARAMPKGDFSYFDASKDYPAEAKSLGIEGVIRVRLVVDEQGKVKSTVLLNK
                     LGHGLDELALERAKKIEFEPAKDTDDKAVSSVVVWTFNMTLPK"
     gene            5729..>6750
                     /locus_tag="VFV99_00535"
     CDS             5729..>6750
                     /locus_tag="VFV99_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5057805.1"
                     /translation="MPAVAWADDPPDDRAIDVQLMDYAIGPKSFFTVANADVADKKQL
                     ALDAFVTFLTKPFTVYTTDGAMDPMITGTRVRVVDSVTAAQLSAAYGINDKIQIGATL
                     PIVFALTGNDFDATKGLMGNSLQVTGTGDLLVEGKMKLWAATNNELRIAGIGEVSLPT
                     SVGSNESQFIGDNLPTLRGKLAATFTKDKISLGANAGFILRKPRTIYSSTVGQQLTFG
                     VAGAVAIQPNFSIIAELFGRGGLESGFALDESPAEVIGGFRLITAKSMAITVGGGTGL
                     DEAIGAPRARFFASVGYAPDVRDSDGDGIQNAADKCPLIPEDKDGFDDRDGCPDDDND
                     GDRRPDT"
BASE COUNT         1147 a         2278 c         2227 g         1098 t
ORIGIN      
        1 gagtcgaccg gccacctcct cacgatcaag gaggtagtcg acggtgtgga caccacgcat
       61 gagatgccgc gcatcttcat gctcgatatg cgacagccga ttggccacgg ctttcgggag
      121 cggcggatga tcaacatccc cgatcccgat gcgttgttca tcatcgagaa cgacgacacg
      181 accgtgccac ccgatcggct cgcgctcccg cgcgtcatct acgatcacct ccgcggtcat
      241 ccgttcgcgt gcggcccgtt gctcggcagc cgcggccagc ccgtcggcgc gctcggcttg
      301 tcgtcgtatc aaggacgcca gccgattccc gacgagctgt tcgagcacgg actgctccac
      361 gcgttcatga atcacctcgg catcgcgatg gagcgcgcga tccataccgc gcgactcgag
      421 cgcctcaacg ccgagctgct cgaagcgcat gccgccatcg cgagcagcgc gcggatcaag
      481 gctgtcggcg agctcgccgc cgcggtcgcg cacgacctca acaacctgtc cggcatcgcg
      541 cttctggcgg tcagcgccgg ccgccattca ccgaaggccg cgttcgacgt gttgccgcgc
      601 atcgagcgcg cgaaccgcac gatcggcgag ctcgtcggcc ggctccagcg cgtcgcccgg
      661 caagcgcatc ccgaagacgg caccggtgat gtcgcgtgcg tgcatcagat cgtcgaagac
      721 atcctcgcga tgatcagccc gctgctccgc gaacgcgcga tcacggtcga cctcgacttg
      781 cagccgacgc ccgcggcgca cgcggacccg agcttcgtgc atcaggtcgt gctgaacttg
      841 atcctcaacg cgcaggacgc gctcgaatcg atcaccggac ggccgcgcaa gctgcgcgtc
      901 ctggtgcgcg gcgacggcga catggtgcgc ctcgaggtca ccgacaacgg gcccggcatt
      961 gcagcggagg tgctgtcgcg cttgttcgag ccgttcgtca ccacgaagca cggcgcgcac
     1021 gcagggctcg gcctcgcggc tgcagccggc tcgctcaagc acttcggcgg cagcatcgag
     1081 ggtcgcaacg tgcccggcgg tggggcgtcg ttcgaggtca tgattcccgc ggcgtcgacg
     1141 cgcagtgaac gaccgctcgc acaaggcgag ccaccgcaac ccgccgtcca gtcacgccgc
     1201 ggcgcgatcc tggccatcga cgacgacgaa gacatcctcg ctgtcgtgcg cgtgtatctc
     1261 gattcgctcg gctacgaggt ccacacgacg accgacgccg agcaggcgct gcagctcgtg
     1321 cccgggcgtt cgttcgatgt cgtgctctgc gatgtcggca tgccgaagca gaacggcctc
     1381 gacgtgtgcc agctgctccg cgaggccggc tatcgcggca agatcgtgct gatgaccggc
     1441 tgggatactc agcgcgtcaa tgtcgatcgc cgctcgcaga gcagcgacgc cgtgctgaag
     1501 aaaccgttcc tcggttccga gctgatcgac ttgatcgacg cgatgctcgc gccgccgaac
     1561 tagttgtccg ctttttccat cgccgccttc gcgtcgggcg gcagctggag cacgatgatg
     1621 tagtcgttca tcggcttcgc gagcgcttcg gcgacgcgac cctcgacgtc gagcaccggg
     1681 cgcagcgaga tcaccttgtc gacgccgcga cgcttgatgc gattgacgat cgcggtgtcg
     1741 tggcagtgac cgttgccggc gagcaagacc aagaggcctt gcggcgttgc cttcgcccac
     1801 ttcgcggcgc cgtcagccat cgtctcgtcc cagagcacct gggccgtata gatgtcatcg
     1861 gcgctcggca tcttcggcgc gtcggaaggt tcttccttct tgtcgtcttt cttctcgtcc
     1921 ttcttcgcgt ctttcttggc ctcgtcgtcg tctttcttgg gcgccgagtg cgcgcccgcg
     1981 ccgcccatct cgttcatcgt gtcgtcgaac catgcgcgat gcgccttgtc gtcgagcacg
     2041 agctcgggaa tctgcttttt ctcgtcgggc gtcagcgacg cgaggccctg gcgcacgacc
     2101 ttcttcgtca gctcgcgcgc ggtattgagc gcgatcagcg tggcgccgac cgcgaccgcc
     2161 gcgttgaccg tcggctggta aaatccccag tcgtagctcc agcgctcctc gtagccggag
     2221 cgcgacaaca gcgcggcctc gtcgatgcgc ttggcggcgt aatcatcgag cacgccctgg
     2281 aacggacgct ggaacatctc catgccgagc gccagcggct tgtgctgcga gacatgcttg
     2341 aacgtctcga gctggaacca gtgatggtgc gggttcgggt gatcttcgcc gacgcacacg
     2401 acgcgcgcgg caccgacctg tgcccagaac gcggcggtgt cgacttgatg gccgctgcga
     2461 tcgagcaccg agtacggcag cgccgctttc tcgatgccgc ctttggcggt gggcgtgggg
     2521 gtcgtcttcg gcgtgccgcc gtacttgccg ccgcacccga tcaagatcgt gagacacaag
     2581 aggcgcgcga ccatgcagcg atgctacacc gcctggcgtg gagctgccgt atctgaccgc
     2641 tgcgttgccg ggcatcggcg gcgtcttgcg tgcgagcccc gaagatttcg tcgtcgacga
     2701 agaacctgcg tattcgccga gcggcagtgg cgaccacgtg ttcgtgcgca tcgagaagcg
     2761 cgggcttaca acggcgatgg ccgtcgagcg catcgcacgc gcactcggcg tcaacgcacg
     2821 cgatatcggc gtcgcgggca tgaaagaccg tcacgcggtc acccgccagt ggctgtcatt
     2881 accgccaccg gtcacgccag aagctgcaag cgcgctcacc ctcgacgacg tgacgattct
     2941 cgaggccaca cgccacggcc acaagctgcg caccgggcac gtgcgtgcga atcggttcgt
     3001 actgcgcgtg cgcgatgtcg ccgccgatgc cgaggacaag gcgcgcgcga ttctcgatgc
     3061 gctcgcggcg cggcccggcg ccccgaactg gtacggcgag cagcgcttcg gccgcgatgg
     3121 cgacaacgcc gagcgtgggc tcgcgatcgt acgcggcgag aagccaccgc gtgatcgcaa
     3181 gctcgcccgc ctgctcgtct cggcgctgca aagcaagctg ttcaacgagt ggctcactgc
     3241 gcgcctcgcc gatggcctct acgcgcgcgt ggtggccggc gacatcttgc acaagacggg
     3301 cggcggccag ttcgtgtgcg aggacccgag cgtggaccag ccgcgcctgc tcgccggcga
     3361 gctatgcatc accggcccga tgttcggcga tcggatgagg gcgccggccg gcgaggcaca
     3421 agaacgcgag caacggatcc tcgcggccgc gggtctaagc gctgacgcct ttgcgtccgt
     3481 gcgggcgatc gcggagggta cgcgccgcga tgccacggtc gaggtcgctg cggccgccgt
     3541 gcgaaccgtc gagccgggcg tggtcgaggt ggcgttcggc ctgcctggtg gcgcgtatgc
     3601 caccacggtc atgcgcgagc tcatgaaatc gagctaggct cctgccgtgc tgtcggagca
     3661 attcgtcgcg ttcgcccagc tcggcgccga atgggtgctg tggctcctga ttggaatgtc
     3721 cgtgctgtcg gtcggcatca tgatcgatcg cgcgctgtgg tttcgccgcc gcgataccga
     3781 caccgccaag ttcacgcgcg agctgcgcgg cgcgttcgag cgcaacgagc tcgaccggat
     3841 cgccaccaag tacaaggacg atccagcggt gccggtgcag gtcgcgctgc gcggcctcgc
     3901 cgagcgaagc caaggtcccg aggcggtcgc cgaggcgatg caaggcgagc gcgtgcgctg
     3961 gcgcaacgcc ggcgatcgcc acctgatcgt gctcggtacg ctcggcaaca acgtgccgtt
     4021 cgtcggtctg ttcggcaccg tgctcggcgt catcaacgcg ttcgagaacc tgcggcagaa
     4081 gacagcgcaa gccgaggacc gcacgctcga cctgattgca gaggcgctcg ccgccaccgc
     4141 gttcggtctg ctcgtcgcga ttcccgccgt gatcgcgttc aactacttct cgcgccgcat
     4201 gcgcgtgcta ctcggcagcg ccgacgaggc cgcgcacacc gtgatgtcga tggtccacgg
     4261 cgagcaccac acgaaggagg cggccaatgg cgggaagtag cctctaccag gacagcgacg
     4321 atccgggcgg catcaccgat atcaacgtca cgccgttcgt cgacgtcgtg ctcgtgctgc
     4381 tcgtcgtgtt catcgtcacc gcaaagctga tcgtggcgcg cggcgtcgac atcgagaagc
     4441 cgaaggcatc gagcggtggc gacgtccaga gcacgctgcg gatcgcggtc aaagccgacg
     4501 gcaccctgtt cgtgaacggc gacgagtaca aggacgacac ggccgcgctc tcgcggatca
     4561 aggagattgc cgcgacgacg tcgaagccca aggcgatcat cgccggcgat cgcaagacgg
     4621 cctacggcgg tgtgatgcgc gccatcgatc tggcgacaca ggcgggcgtg cccatcgccc
     4681 tcgagaacga acgcccttag tcgagcttcg gacctcgggc ttcggacgca ggccaaagac
     4741 tccggacaaa ccgtcacatg ccgggcagag cgtctctgct aggctcgaca cgtgcgcctc
     4801 gactcgccgt ttgtactcgc gaccgcaggc acactcgcga tccatctgtt actggcggtc
     4861 gccggcgacg cgatcgtcgt gctctatccg cacaaacagt acgaagaggc gccgcgggtc
     4921 gagctgatcg acatcgaggt cccgccggtc gtgaagccgc cgcccccgcc cgtccagccc
     4981 gagcggccgg aaccggtgaa agagacgcgg ccggtgccgc aaaccgtcca gaaggtcgtg
     5041 cggcagcagc ccaccgcgcc gccgccaccc gagaacgagc cgccgccgcc cgtcgacacg
     5101 ccgccgtcgg gcggtgcacc gctcgcgacg atggaaggtg tgatcccgtc cgcgactggt
     5161 gttcccgtcg cggtcggcaa gcgcacgagc gagcacgttg gccgcggtgg caccggtacg
     5221 gggacaggcg ccggctcggg tacgggttcc tctgacgagc cgccgaagcc ggtctcggtc
     5281 gcgacgatca aagcccgcgc gatgccgaag ggcgacttca gctacttcga cgcgagcaag
     5341 gactaccctg ccgaagcaaa gtcacttggg atcgagggcg tgatcagggt gcggctcgtc
     5401 gtcgacgaac aaggcaaggt gaagtcgacg gtgctgctca acaagctcgg ccacgggctc
     5461 gacgagctcg cgctcgagcg ggcgaagaag atcgagttcg agccggcaaa ggatacggac
     5521 gacaaggccg tgagctcggt cgtcgtctgg acgttcaaca tgacgctgcc aaagtgaagc
     5581 gcgcgcaaat cgagcgcagc taacccagcc gcgacgctcg cagatcggcc gaatagctat
     5641 cccgcccata ccccggtcac atgtgggtcg gagtagaatg ctcgcaatgt gcaaactccg
     5701 gggaacggtt gcggttctgg ctctttctat gccggccgta gcatgggcag acgacccgcc
     5761 cgatgacaga gcgatcgatg tccagctgat ggactacgcg atcggaccga aatcgttctt
     5821 cacggtcgcg aacgccgacg tcgcggacaa gaaacagctc gcgctcgacg cgttcgtcac
     5881 gttcttgacc aagccgttca ccgtctacac gaccgacggc gcgatggatc cgatgatcac
     5941 gggcacgcgc gtccgcgtcg tcgactcggt gaccgcagcg cagctctcgg ccgcgtacgg
     6001 catcaacgac aagatccaga tcggcgcgac gctaccgatc gtcttcgcgc tcacgggcaa
     6061 cgacttcgac gcaacgaagg gcttgatggg caattcgctg caggtcaccg gcaccggcga
     6121 cttgctcgtc gaaggcaaga tgaagctgtg ggccgcgacg aacaacgagc tgcggatcgc
     6181 cggcatcggc gaggtctcgc tgccgacgag cgtcggtagc aacgagtcgc agttcatcgg
     6241 tgacaacttg cctacgctgc gcggcaagct cgccgcgacg ttcacgaagg acaagatctc
     6301 gctcggtgcg aacgcggggt tcatcctgcg caagccgcgc acgatctact cgagcaccgt
     6361 cgggcagcag ctgacgttcg gcgtcgccgg cgcggttgcg atccaaccca acttttcgat
     6421 catcgccgag ctgttcggtc gcggtggtct cgaatcgggc ttcgcattgg acgagagccc
     6481 ggcagaagtg atcggcggct tccggctgat cacggcgaag tcgatggcga tcacggtcgg
     6541 cggtggaact ggactcgacg aagcgatcgg cgcgccgcgc gcgcggttct tcgcatcggt
     6601 cggttacgcg cccgacgtgc gcgacagcga cggtgatggc atccagaacg cggccgacaa
     6661 gtgcccgctc attcccgaag acaaggacgg cttcgacgat cgcgatggct gtcccgacga
     6721 cgacaacgac ggcgatcgcc gccccgacac
//
LOCUS       DASUFX010000013         5352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_141226,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000013 DASUFX010000000
VERSION     DASUFX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141226"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFV99_00540"
     CDS             <1..471
                     /locus_tag="VFV99_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5057806.1"
                     /translation="SFQGQIDAVPILALFLPMIVSTGGNSGSQASTLVIRAMALGEVR
                     GADWWLILRRELITGLALGLVLGALALLRVAIWGTWGAYGPHFHHLALVAGLSVIGCV
                     LCGTLAGAMLPFVLKKFGQDPASASAPFVATLVDVSGILIYFAIANIVLRGILL"
     gene            492..1376
                     /locus_tag="VFV99_00545"
     CDS             492..1376
                     /locus_tag="VFV99_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057807.1"
                     /translation="MFATRVYHYRDPAAVILGLKELRKQGLTPRGLLFVALDPRGETY
                     IAVPEDLDAVASIKVGDKLSLVPPWEGRYFHFDAVHRLPGDTVLWNGDRRLGDTGSAP
                     EVACAISEWLKGSSAKNVFLGCAAHVPGSWWTVDHLSAVIDLHALGLLDCVITTTGVL
                     ARRIDDARLFHLEWQALAQNKTPTEGWQEVFKSEMGNILLVERRVLQYRLVLTCERGL
                     VEIDVSHLPDLVIETARVPMRSGFGVVGRIDGGAFAVTAGTIEPWGLTNMSPAMLVGS
                     PTASLLDLPKTLRAMPLE"
     gene            1435..2898
                     /locus_tag="VFV99_00550"
     CDS             1435..2898
                     /locus_tag="VFV99_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057808.1"
                     /translation="MTPQKVAPDEVVACVRPYAGRGCAPPGEVAPRVSALIAAGCVVT
                     VAIALLGMDSMWGSADWIEQSPVWGVGRSEFLYTHGLPLLLMIAMALAFAPRFDVSRV
                     LRIAVLLPVFHLAAIVIAAFAWTAIHADIDAVNEAQGWKVSYLPNIGLPSSAALVVAF
                     GAVFAIAITIKWRRGEWAHAGVMLTLSYLLLVGLWLPILSRLSISSSNAFNAANAFRE
                     NCDFGYGCGYRYYWAWQFNHRILSREAFTVLAIVPPMLVAIAFTAIAFRSPRLFNRLR
                     AWPRLAVKLLLGGGVLAALTLPDEGWLLYLESSYLVLFAVILAVGALITLTVITRLGS
                     LASHFSFGRLPKVEGTIARDEQDEVARFEVTSWLRGPRLAVRSFVVATPNGNVPLDGV
                     TVLARVPASTTALDVGEHAPVLAPGDRVIIGGRKGRTGAADPFRTIEATDVAAVAGAD
                     ARAYRFSDVALVVWRPAVAYLAILVAVALPYLSIVLT"
     gene            complement(2951..3157)
                     /locus_tag="VFV99_00555"
     CDS             complement(2951..3157)
                     /locus_tag="VFV99_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057809.1"
                     /translation="MLTFDSIPQARVVRKGGQQFAPPPAPRTEDEPSKLMEALNLAIG
                     LWPLTAIVLLATGLLIMAGNNGAP"
     gene            3236..4945
                     /locus_tag="VFV99_00560"
     CDS             3236..4945
                     /locus_tag="VFV99_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX5057810.1"
                     /translation="MARSVADLSQGFRSAYLDDKQLTAQLHAWADAYPTLVRLTSLAK
                     TPEGRDVWLLTIGPDPDRIRPAVWADGNLHAAELAGSSVALSIAEDAIRAHVEPQSLD
                     LPQPIIDRMREVLFYVVPRISPDGAECVLKTGRSVRSVPRDPRVERGQPRWIPGDVDG
                     DGLSFAMRVKDPGGELVESREFPGLLVERTVEDDGPFYKLYPEGMIEHFDGKHVPSPF
                     FLGDNPIDLNRQFPWSWAPGHEQIGAGPFPASEPEARGILEFTSKHPEIFAWCNYHTF
                     GGVLIRPLGHAPDSKMEPEDLAIFRQLEAWMTEFTGYPTVSGFEEFLYEPDKPIHGDL
                     SDYAYNQRGALAYVIELWDLFKRLGMEKPPKFVQYYERVSRADVIKLAWWDRDENEGR
                     CFPAWRAFEHPQLGPVEIGGIDPRVGMWNPPLHELANVCATQTQVFVRLASLAPRVRI
                     AETNRYPLPGGIVRVELKVINDGYLASYGLPSAKKLDFNEPLYATAKGDLVDAGMAYQ
                     SLGHLDGWGHGLHTGANLPAYPGTRGSTNAAWATYLVKGGGVLEVRIGSARTGFVTAK
                     IEV"
     gene            5008..>5352
                     /locus_tag="VFV99_00565"
     CDS             5008..>5352
                     /locus_tag="VFV99_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5057811.1"
                     /translation="MATYAIGDIQGCMASLERLLSLIDFSPAEDRLWLVGDLVNRGPR
                     SLDVLRWAYDNDRVITCVLGNHDIHLLARAAGAAQPKKRDTLDDVLNARNNEKWLSWL
                     RDRPLFYAENGFG"
BASE COUNT          797 a         1805 c         1765 g          985 t
ORIGIN      
        1 tcattccaag gccagatcga tgcggtcccg atcctcgcgc tgttcctgcc catgatcgtg
       61 tcgaccggtg gcaactcggg ctcgcaagcg tcgacgctcg tcattcgcgc gatggcactc
      121 ggcgaggtcc gcggcgcgga ctggtggctg atccttcgcc gcgagctcat caccggcctc
      181 gcgctcggcc tcgtgctcgg tgccctcgcg ctgttgcgcg tcgcgatctg gggtacctgg
      241 ggcgcctacg gcccgcactt ccaccacctc gcccttgtcg ccggcctgtc ggtcatcggt
      301 tgcgtcctct gcggcacgct cgccggcgcg atgctgccgt tcgtactcaa gaagttcggc
      361 caggaccccg ccagcgcatc agcaccgttc gtcgccacgc tcgtcgatgt cagcggaatc
      421 ctcatctact tcgccatcgc gaatatcgtt ctgcgtggaa tcctcctcta aggtctgaga
      481 taaccgcggc gatgttcgcc acccgcgtct atcattaccg tgacccggcg gcggtcatcc
      541 tcggtctcaa ggagctgcgc aagcagggcc tgaccccgcg cggccttctg ttcgtcgcgc
      601 tcgatccgcg tggcgagacg tacatcgcgg tgcccgagga tctcgatgcg gtcgcgagca
      661 tcaaggtcgg cgacaagctc tcgctcgtgc cgccgtggga aggacgctac ttccacttcg
      721 acgccgtgca ccggctgccc ggcgacaccg tgctgtggaa cggcgatcgc cggctcggcg
      781 acaccggctc ggcgcccgag gtcgcgtgcg cgatctcgga gtggctcaaa ggctcgtcgg
      841 cgaagaacgt gttcctcggc tgcgccgcgc acgtgcccgg ctcgtggtgg acggtcgatc
      901 acctgtcggc ggtcatcgat ctgcacgcgc tcggcctgct cgactgcgtc atcacgacga
      961 ccggcgtgct cgcgcgcagg atcgatgatg cgcgcctgtt ccatctcgag tggcaagcgc
     1021 tcgcgcagaa caagacgccg accgaaggct ggcaagaagt cttcaagtcg gagatgggca
     1081 acatcctgct cgtcgagcgc cgcgtgctgc agtaccggct cgtgctgacg tgcgagcgcg
     1141 ggctcgtcga gatcgacgtg tcccacctgc cggatctcgt catcgagacc gcgcgcgtgc
     1201 cgatgcgaag tggctttggc gtcgtcggcc gcatcgacgg cggtgcgttc gccgtgacgg
     1261 ctggcaccat cgagccctgg ggtctgacga acatgagtcc agcaatgctc gtgggttcgc
     1321 cgacggcgag cttgctcgat ctgccgaaga cgctccgcgc catgccgctc gagtaaccgc
     1381 cctggaataa gaaacctgcg gccgtcggtc ggtgtcctcg ctaccatgct ggacatgacg
     1441 ccgcagaagg ttgctccgga cgaggtcgtg gcgtgcgtga ggccatacgc tggccgcggc
     1501 tgcgcgccac ccggtgaggt cgcgcctcgc gtgagcgcgc tgatcgcggc gggctgcgtc
     1561 gtcaccgtcg cgatcgcgct gctcggcatg gactcgatgt ggggctcagc cgactggatc
     1621 gagcagtccc cggtctgggg cgtcggtcga tccgaattct tgtacacgca tggactgccg
     1681 ctgttgctga tgatcgcaat ggcgctcgcg tttgcgccgc gattcgacgt ctctcgtgtg
     1741 ctgcgcatcg ctgtgctatt gccggtcttc catctcgccg cgatcgtgat cgctgcgttc
     1801 gcgtggacgg caatccacgc ggacatcgat gccgtgaacg aggcgcaggg ttggaaggtc
     1861 agctaccttc cgaacatcgg cttgccgtcg agtgccgcgc tcgtcgtcgc gtttggcgcc
     1921 gtgttcgcga tcgcgatcac gatcaagtgg cggcgcggcg agtgggccca cgccggcgtg
     1981 atgctcacgc tcagctactt gctgctggtc gggctgtggc tgccgatcct gtcgcgcctg
     2041 tcgatttcat cgtcgaatgc gttcaacgct gccaacgcgt tcagagagaa ctgcgatttt
     2101 ggctacggct gtggctaccg ctactactgg gcatggcagt tcaaccaccg catcctcagt
     2161 cgcgaggcgt tcaccgtgct cgcgatcgtg ccgccgatgt tggtcgcgat cgcattcacg
     2221 gcgatcgcgt tccgctcccc tcgcctgttc aatcgcctgc gcgcgtggcc acggctcgcg
     2281 gtcaagctgc tgctcggcgg cggtgtgctc gccgcgctca cgctccccga cgaaggctgg
     2341 ctgctctacc tcgaatcgtc gtacctcgtg ttgttcgccg tgatcctcgc ggtcggcgcg
     2401 ctgatcacgc tgaccgtcat cacgcgcctc ggttcgctcg catcgcactt cagcttcggc
     2461 cgcttgccga aagtcgaagg cacgatcgcg cgtgatgaac aagacgaggt cgcgcgcttc
     2521 gaagtgacga gctggttgcg cggaccgcgc ctggcggtgc gctcgttcgt cgtcgcgaca
     2581 ccgaacggca acgtgccgct cgacggggtg accgtgctcg cgcgtgtgcc ggcaagcacg
     2641 acggctctcg acgtcggcga gcacgccccg gtgctcgcac ccggcgatcg cgtgatcatc
     2701 ggcggccgca aaggccggac gggtgcggcc gatccgttcc gcacgatcga agcgaccgac
     2761 gtcgcggccg tcgccggtgc cgacgcgcgc gcctatcgct tcagcgacgt cgcgctcgtc
     2821 gtctggcggc cggcagtcgc atacctagcg atcctggtcg cagtcgcgct gccgtacctg
     2881 tcgatcgtcc ttacctgagt acgcgcacgt gcggctccgg ttcggtcgaa agaccgaagg
     2941 ccgaagcctg ttacggcgcg ccgttgttgc cggccatgat cagaaggccg gtcgcgagca
     3001 gcacgatcgc cgtgagcggc cacagaccga tcgcgaggtt gagcgcttcc atcagcttgc
     3061 tcggctcgtc ctcggtgcgc ggcgcgggcg gcggcgcgaa ttgctgaccg cccttacgaa
     3121 caacacgggc ttgtggaatc gaatcgaagg tcagcatggg catttcccct agtattacgt
     3181 tccagagaga cgctcggcac cctgcctttc ttcacgggtg taagctgcgt cacccatggc
     3241 gcgttcagtt gccgatctct cccagggttt tcgttcggcg tacctcgacg acaagcaact
     3301 caccgcacag ttgcacgcgt gggcggatgc atatccgaca ttggtgcggc tgacgtcgct
     3361 ggccaagacg cccgaggggc gcgacgtctg gctcctgacg atcgggcctg acccggatcg
     3421 gatccgtccg gccgtgtggg ccgacggcaa cctgcacgcc gcggagctcg ccggctcgag
     3481 cgtcgcgctg tcgatcgccg aggacgcgat ccgcgcgcat gtcgagccgc agagtctgga
     3541 cctgccgcag ccgatcatcg atcgaatgcg cgaggtgctg ttctacgtcg tgccgcgcat
     3601 ctcgcccgac ggcgccgagt gcgtgctcaa gaccggccgc agcgtgcgct cggtgccgcg
     3661 cgatccacgc gtcgagcgtg ggcagccgcg ctggattccc ggcgacgtcg acggcgacgg
     3721 cctgtcgttc gcgatgcgcg tcaaggatcc gggcggcgag ctcgtcgagt cgcgcgagtt
     3781 cccgggcttg ctcgtcgagc gcaccgtcga ggacgacggc ccgttctaca agctgtatcc
     3841 cgaagggatg atcgagcact tcgacggcaa gcacgtgccg tcgccgttct tcctcggcga
     3901 caacccgatc gacttgaacc gccagttccc gtggtcgtgg gcgccgggcc acgagcagat
     3961 cggcgctggc ccgtttccgg cgagcgagcc cgaggcgcgc ggcatcctcg agttcacgtc
     4021 gaagcatccg gagatcttcg cgtggtgcaa ctaccacacg ttcggcggcg tgctgatccg
     4081 gccgctcgga cacgcgcccg attcgaagat ggagccggag gacctcgcga tcttccgcca
     4141 gctcgaagcg tggatgaccg aattcaccgg gtaccccacc gtgtccggct tcgaggagtt
     4201 tctctacgag ccggacaagc cgatccacgg cgacctctcc gactacgcgt acaaccagcg
     4261 cggtgcgctc gcgtacgtga tcgagctgtg ggacctgttc aagcgactcg gcatggagaa
     4321 gccgccgaag ttcgtccagt actacgagcg cgtgtcgcgc gctgacgtca tcaagctcgc
     4381 gtggtgggat cgcgacgaaa acgaaggccg ctgctttccg gcgtggcgcg cgttcgagca
     4441 tccgcagctc ggccccgtcg agatcggcgg catcgatccg cgcgtcggca tgtggaatcc
     4501 gccgctgcac gagctcgcga acgtgtgcgc gacgcaaacc caggtgttcg tgcgcctcgc
     4561 gtcactcgcg ccgcgcgtgc gcatcgccga gacgaatcgc tatccgttgc cgggcggcat
     4621 cgtgcgtgtc gagctcaagg tgatcaacga cggctacctc gcgagctacg gcctgccgag
     4681 cgcgaagaag ctcgacttca acgagccgct gtacgcgacc gcgaaaggcg acctcgtcga
     4741 tgcgggcatg gcgtatcagt cgctcggtca tctcgacggc tggggccatg gccttcacac
     4801 cggcgcgaac ctgccggcgt atccaggcac ccgcggctcg acgaacgcag cgtgggccac
     4861 gtacctcgtc aagggcggcg gcgtactcga ggtgcgcatc ggcagcgcgc gcaccggctt
     4921 cgtgaccgcg aagatcgagg tctgaaagca ctggtgtgtc agcggcgctc tctctgcgag
     4981 ctcgtcgctg cgtggtagcg tagcggcgtg gccacgtacg ccattggcga catccaaggc
     5041 tgcatggcca gcctcgagcg gttgctctcg ctcatcgatt tttcgcctgc ggaagatcgg
     5101 ctgtggctcg tcggtgacct cgtcaatcgc ggaccgcgct cgctcgacgt cttgcgctgg
     5161 gcctacgaca acgatcgcgt gatcacgtgc gtgctcggca accacgacat tcacttgctc
     5221 gcgcgcgccg ccggcgccgc acaaccgaag aagcgcgaca cgctcgacga cgtgctgaat
     5281 gcgcgcaaca acgagaaatg gctgtcctgg ctgcgcgacc ggccgctgtt ctatgccgag
     5341 aatggtttcg gc
//
LOCUS       DASUFX010000014         5522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1252560, whole genome shotgun sequence.
ACCESSION   DASUFX010000014 DASUFX010000000
VERSION     DASUFX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252560"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFV99_00570"
     CDS             complement(<1..542)
                     /locus_tag="VFV99_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5057812.1"
                     /translation="MTAFVLGATGFVGREVVRQLCVRGTKTFAHVRPDSSKVAEWRTT
                     FGELGAEVDTTAWDIAGLAARWRELAPAQIYVCIGTTRNKAKSDNVQGDIYEAVDLGL
                     TKLAVDAARASETKPRIVYLSSVGADVNARSAYLRARGKAEDVVKNSGLPWVIARPSI
                     ITGDRDESRFGEKAAGVVGDG"
     gene            complement(539..1483)
                     /locus_tag="VFV99_00575"
     CDS             complement(539..1483)
                     /locus_tag="VFV99_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057813.1"
                     /translation="MAKPLEYNATLAERIDLTDALSIFRVKPDQMPAERPWFVPGQYC
                     VLGANNQEKPELGSVRRSMSIASAPEDDGPIEFYIRWVAKPESDNPLTHLLWKLKTGD
                     RMYMRAVAAGVFTVKDTVGVDDPRYRVMVAAGTGSAPFLSMVRSELRRNPKADLSKYV
                     LLHGASYPADLGYRDELQHMIETNQLKYWGTVSRPKEAPDWKGDTGRVESFFEGARLD
                     DLEKRLGLPAGGFNPKNVVIFICGLTGTITGTIIPLLDRGFVPDFKRIREALAVPSDV
                     KDSVFYEQYDTEPVINVKDPAVVEPLRARMQAGLAKLG"
     gene            complement(1517..2119)
                     /locus_tag="VFV99_00580"
     CDS             complement(1517..2119)
                     /locus_tag="VFV99_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX5057814.1"
                     /translation="MTSLIRSVRALALLGWIAGCALPYTGGARAVHPTQLGAGWLTAA
                     ETPVVKQKQQTDCGLAALAMVAGAWGRHWSVDDLAHRIPPGQHGVKLGALRDLARERG
                     FEAFAIRASRDDLKNELSKGRPVLVGLMLPHDRKSNRSHYEVAIALNTQDGTVITIDP
                     ATGEWMRRSPKVLDIEWKAAGYAALVVTGDKANTNVGDAR"
     gene            complement(2135..2401)
                     /locus_tag="VFV99_00585"
     CDS             complement(2135..2401)
                     /locus_tag="VFV99_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057815.1"
                     /translation="MLLRNLALATTLAVGAPAVALAAPDAPAAVQTVSVKAAPKASTS
                     DATSYAQREKQDQKAADFQGGDLVVVGISGGAILVLLILLLILV"
     gene            complement(2486..4144)
                     /locus_tag="VFV99_00590"
     CDS             complement(2486..4144)
                     /locus_tag="VFV99_00590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5057816.1"
                     /translation="MQSPSVPARLRRSRHLSWFAHMGAVYLFHDLYGYLMEMSPDIAQ
                     MIESFSDGVDTEETIAYYQGKFGDADPRQFVDVLASHAVLVDPAEDEIEALWAFVPLK
                     AKWNVWQRRDDRLTLWTAWGDKPEQQIFLDEEETKIWDAFAGEKRLIEMRHHYDNDKL
                     LRLVRRLTHSDVQALKLSMMPWSIYAKRPALAPAYLSSTMPYARWQPGTQVPPAVSLT
                     DYHVHDITKADEQFDHQETTLSHLLRIPHPALANRTYGQALADALLARNAIPQATVRV
                     LEIGAGLGYVARHVIDRLRESGRTVAYTIVELSPALAAAQRERLDSDATWIEGDALNV
                     ALPDGAFDVILSNEMVGDLPAKQLSRVDIGLELAGTGTADRDKVRAIAPLADAVNLDD
                     APEPFYLQTGAFELVNKIARWLAPGGTAVVTEFGDANAWPKLSTQLDHPELSTHFGHL
                     QQIARADGLEASIEFVIDLLDFDRSQQGLATTRSHFRALRALATHAGVELPKIGYTRE
                     LLAHTVAGKLDLATVGDLRFDRIEDRLMGLVPHEFKALLANKPS"
     gene            4183..5331
                     /locus_tag="VFV99_00595"
     CDS             4183..5331
                     /locus_tag="VFV99_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5057817.1"
                     /translation="MAYMFETEEHAAIRATARRFAQSQIAPHGAQWEEDEEFPIELYK
                     AAATAGISGIGYPEAVGGQGGDLSHVLVACDELIVAGRSVGTVVGLGTHGIALPPIVR
                     FGTPEQHKRFVAPCLREGKISALGITEPGGGSDVASLRTKAVRDGDHYVISGAKTFIT
                     SGTRADFVTCAVRTGGDGHAGISLVVIERGTPGFEVSKKLKKTGWWASDTAELHFDNC
                     RVPVANRIGPEHGAFPMIMMNFAGERLMLAGQCVAIAELAYRESINYARERQAFGKPL
                     TGFQVTRHKLADMASRIAAARSLTGEVCLRMMRGENVEVAGLAAKAKNIATDMCSFVC
                     DQAVQIHGGYGYMRETLVERLYRDARLYPIGGGTREIMNEVIAKTEGY"
     gene            5332..>5522
                     /locus_tag="VFV99_00600"
     CDS             5332..>5522
                     /locus_tag="VFV99_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057818.1"
                     /translation="MSEETKGRGSPRALRALPPALLLAFAGWVGWQIANVIFMLTAKE
                     PWSAVRFHLVNEGLGFACES"
BASE COUNT          953 a         1747 c         1867 g          955 t
ORIGIN      
        1 ccgtcaccga cgacaccggc cgccttttca ccgaaccggc tctcgtcgcg atcgccggtg
       61 atgatcgacg ggcgcgcgat cacccacggc agtcccgaat tcttcacgac gtcttcggcc
      121 ttgccgcgag cgcgcaggta cgcggagcgc gcgttcacgt cggcaccaac cgacgacagg
      181 tacacgatgc gcggtttcgt ttcacttgca cgtgccgcgt cgaccgcgag cttcgtaagc
      241 ccgagatcga cggcctcgta gatgtcgccc tggacgttgt cgctcttcgc tttgttgcgc
      301 gtcgtgccga tgcacacgta gatctgcgcc ggtgcaagct cgcgccagcg cgcggcgagg
      361 ccagcgatgt cccacgccgt cgtgtccacc tcggcgccga gctcaccaaa tgtggtgcgc
      421 cactcggcga ccttcgacga atcgggccgc acgtgcgcga acgtcttcgt gccgcgcacg
      481 cacagctggc gaacgacctc gcggccgacg aaaccggtgg caccgaggac gaacgcggtc
      541 atccgagctt cgccaggccc gcctgcatgc gcgcccgcag tggctcgaca acagccgggt
      601 ccttgacgtt gatgacgggc tcggtgtcgt actgttcgta gaacaccgag tccttgacgt
      661 cggagggcac tgcgagcgct tcgcggatgc gcttgaagtc cggcacgaag ccgcggtcga
      721 gcagcggaat gatcgtgccc gtgatcgtgc cggtgaggcc gcagatgaag atcacgacgt
      781 tcttcgggtt gaagccgccc gcgggcaggc cgagccgctt ctcgagatcg tcgagccgtg
      841 cgccctcgaa gaacgactcg acgcggccgg tgtcgccctt ccagtccggc gcctctttgg
      901 ggcggctcac cgtgccccag tacttgagct ggtttgtctc gatcatgtgc tgcagctcgt
      961 cgcgatagcc gaggtcggcc ggatacgacg cgccatggag cagcacgtac ttcgaaaggt
     1021 ccgccttcgg attgcggcgc agctcgctgc ggaccatcga gaggaacggc gccgagcccg
     1081 tgcccgcggc gaccatcacg cgatagcgcg ggtcgtcgac gccgacggtg tccttgacgg
     1141 tgaatactcc ggccgcgacc gcgcgcatgt acatccggtc gccggtcttg agcttccaga
     1201 gcaggtgcgt gagcggattg tctgactccg gcttcgcaac ccagcgaatg tagaactcga
     1261 tcggcccgtc atcctcgggc gcggacgcga tcgacatcga ccggcgtacc gagccgagct
     1321 ccggcttctc ctggttattg gcgccgagca cgcagtactg accgggaacg aaccagggcc
     1381 gctcggccgg catctgatcc ggcttgaccc gaaagatcga gagcgcatcg gtgaggtcga
     1441 tgcgctcggc gagcgtggcg ttgtactcga gaggcttggc catcggccgc gacgctagca
     1501 cgtcacggca ggcacttcac cgagcgtctc caacgttcgt atttgccttg tccccggtaa
     1561 ccacgagcgc cgcgtaaccg gccgccttcc attcgatgtc gagcactttg ggcgagcgac
     1621 gcatccactc accggtggcc ggatcgatcg tgatcaccgt gccatcctgg gtgttgagcg
     1681 cgatcgcgac ctcgtagtga ctgcgattgg atttgcggtc gtgcggcaac atgagcccga
     1741 cgagtacggg ccgtcctttc gacagctcgt tctttagatc gtcgcgtgac gcacgaatcg
     1801 cgaacgcttc gaatccgcgc tcgcgcgcga gatcacgaag tgcaccgagc ttcacaccgt
     1861 gctgtccagg cggaatgcga tgcgcgagat cgtcgaccga ccaatgacgc ccccaggcgc
     1921 cggcgaccat cgcgagcgca gcgagaccgc aatcggtctg ctgtttctgc ttaacaacgg
     1981 gtgtttcagc ggctgtgagc caaccggctc cgagctgcgt gggatgcacc gcgcgcgcgc
     2041 cacccgtgta gggaagcgcg cagcctgcga tccaacccag cagtgcgaga gctcgcaccg
     2101 aacggatgag cgaggtcatg ggcattgcgg ggacctagac gaggatgagg agcaggatca
     2161 gcagcacgag aatcgcgccg cccgagatgc cgacgaccac gaggtctccg ccctggaagt
     2221 ctgccgcctt ctggtcctgc ttctcgcgct gggcgtagct cgtcgcgtcg gatgtgctgg
     2281 ccttcggggc agctttcacg gacacggtct gcaccgccgc cggtgcgtca ggagcagcaa
     2341 gggcaaccgc cggcgcacca acggcaagag tcgtggcgag agcaagatta cgaagcaaca
     2401 tgatgatcct ccaatcagtt gcttggaggg agttgcaacg gtcaggccca ctacgtagtg
     2461 cgtgcgccga cgcgcggtgc gccgactagc tcggtttgtt cgcgagaagt gctttgaact
     2521 cgtgtggcac gaggcccatc agtcggtcct cgatgcggtc gaagcgaaga tcaccgaccg
     2581 tggcgaggtc gagcttgcct gcaacggtgt gcgcgagaag ctcacgcgtg tagccgatct
     2641 tcggtagctc gacgccggca tgtgtggcga gggcacgcag cgcgcggaag tggctgcgcg
     2701 tcgtcgcgag accttgctgc gagcgatcga agtcgagcag atcgatcacg aactcgatgg
     2761 atgcctcgag gccgtcggcg cgcgcgatct gctgcagatg accgaagtgc gtcgagagct
     2821 ctgggtgatc gagctgcgtc gacagtttcg gccacgcatt ggcatcgcca aattccgtga
     2881 cgacggccgt gccgccgggc gcgagccagc gcgcgatctt gttcacgagc tcgaacgcgc
     2941 cggtctggag gtaaaacggc tccggcgcgt cgtcgagatt gacggcgtcg gcgagtggcg
     3001 cgatcgcgcg caccttgtcg cgatcggcgg tccccgtgcc ggcgagctcg aggccgatgt
     3061 cgacccgcga cagctgcttc gcgggcaggt cgccgaccat ctcgttgctg aggatgacgt
     3121 cgaacgcacc atcgggtagc gcgacattca gcgcgtcgcc ttcgatccat gtcgcgtcgc
     3181 tatcgaggcg ctcgcgttgt gccgcggcga gcgcgggcga cagctcgacg atcgtgtacg
     3241 cgacggtacg gccgctctcg cgcagtcgat cgatcacgtg acgggcgacg tagccgaggc
     3301 cggcgccgat ctcgagcacg cgcacggtgg cttgcggaat cgcattgcgt gcgagtaacg
     3361 catcggcgag cgcttgaccg tacgtgcgat tcgcgagcgc gggatgcggg atgcgcaaca
     3421 ggtgcgagag cgtcgtctcc tgatgatcga actgctcgtc ggctttcgtg atgtcgtgga
     3481 cgtgatagtc ggtgaggctc actgccggcg gcacctgcgt accgggctgc cagcgcgcgt
     3541 acggcatcgt cgatgacaga tacgcaggcg cgagcgcggg acgcttcgcg tagatcgacc
     3601 acggcatcat cgacagcttg agcgcctgga catccgagtg cgtgaggcgg cgcacgaggc
     3661 ggagcaactt gtcgttgtcg tagtggtgac gcatctcgat caggcgcttc tcgcccgcga
     3721 acgcgtccca gatcttcgtc tcctcctcat cgagaaagat ctgctgctcg ggcttgtcgc
     3781 cccatgcggt ccacagcgtc agccggtcgt cccgccgctg ccagacgttc cacttcgcct
     3841 tgagcggcac gaacgcccac agcgcttcga tctcgtcctc ggccggatcg acgagcactg
     3901 cgtgcgacgc gagcacgtcg acgaactggc gcggatcggc gtcgccgaac ttgccctggt
     3961 agtaggcgat ggtctcctcg gtgtcgacgc cgtctgagaa actctcgatc atctgtgcga
     4021 tgtcggggct catctccatc aggtagccgt aaagatcgtg gaacaaatac accgcgccca
     4081 tgtgggcgaa ccacgacagg tggcgggaac gccgaaggcg cgccggcaca ctcggagatt
     4141 gcatgcccct tcctatcatc atcggggtat ggtcgcgcgc tcatggcgta catgttcgag
     4201 accgaagagc acgcagcgat ccgcgcgact gcgcgtcgct tcgcgcaaag tcagatcgct
     4261 cctcacggtg cgcagtggga agaggacgaa gaattcccga tcgagctgta caaagccgca
     4321 gcgaccgcag ggatcagcgg catcggttat ccggaagccg tcggcgggca aggcggcgac
     4381 ttgagccacg tgctcgtcgc gtgtgacgag ctgatcgtcg cgggtcgctc ggtcggcacc
     4441 gtcgtcggcc tcggcacgca cggtatcgcg ctgccgccga tcgtgcgttt cggtacgccg
     4501 gagcagcaca agcgattcgt cgcgccgtgc ttgcgcgagg gcaagatcag cgcgctcggc
     4561 atcaccgagc cgggcggcgg gagcgacgtc gcgtcactac gtacgaaggc agttcgcgac
     4621 ggcgatcatt atgtgatcag cggtgcgaag acgttcatca cgtcgggcac gcgcgccgac
     4681 ttcgtgacgt gtgcggtgcg caccgggggt gacgggcacg cgggcatttc actcgtcgtg
     4741 atcgagcgcg gcacgcccgg cttcgaggtc agcaagaaac tgaagaagac cggctggtgg
     4801 gcaagcgaca ccgccgagct gcacttcgac aactgtcgcg tgccggtcgc gaatcgcatc
     4861 ggcccggagc acggcgcgtt tccgatgatc atgatgaact tcgccggcga gcgcttgatg
     4921 ctcgcgggcc agtgcgtagc gatcgccgag ctcgcgtatc gcgagtcgat caactacgcg
     4981 cgcgagcgcc aggcgttcgg caagccgctc accggctttc aggtcacgcg tcacaagctt
     5041 gccgacatgg cgagccggat cgcggcggcg cggtcgctga ccggcgaagt gtgcctgcgc
     5101 atgatgcgcg gggagaacgt ggaggtcgcg ggacttgccg cgaaggcgaa gaacattgcc
     5161 accgacatgt gcagcttcgt gtgcgatcaa gcggtccaga tccatggcgg ctacgggtac
     5221 atgcgcgaaa cgctcgtcga gcggctgtat cgcgacgcgc gcctgtatcc gatcggcggc
     5281 ggcacgcgcg agatcatgaa cgaagtgatc gcgaagacgg aggggtacta gatgagcgag
     5341 gagacgaagg gacggggatc accaagggcg ctacgcgcgc tgccaccggc actgctgctc
     5401 gcgttcgcgg gctgggttgg ctggcagatc gcgaacgtga tcttcatgct cacggcgaag
     5461 gaaccgtgga gtgccgttcg ctttcatctc gtcaacgaag gcctcggttt cgcgtgcgaa
     5521 tc
//
LOCUS       DASUFX010000015         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1113596, whole genome shotgun sequence.
ACCESSION   DASUFX010000015 DASUFX010000000
VERSION     DASUFX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113596"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFV99_00605"
     CDS             complement(<1..370)
                     /locus_tag="VFV99_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057819.1"
                     /translation="MRWLLALALVGCIDDDLVECGDRLCPQGTACDLAHHSCVDPEQL
                     TACEGKSEFESCETSSVAMGSCFAGVCLPAGCGNGELELALGERCDDGNTVSGDGCSA
                     DCVSREVCGDGYADKLGGEEC"
     gene            complement(390..1538)
                     /locus_tag="VFV99_00610"
     CDS             complement(390..1538)
                     /locus_tag="VFV99_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5057820.1"
                     /translation="MALDLSLTEEQNALVSTAREFTRKEIIPKAGHWDETGEFPRDVM
                     KKAWETGLMNIEVPAEYGGLGGSCVDNCLVQEEVAFGCAGVNTSMAANSLGAMPLLIA
                     GNDEQKKKYLSRLTSELMFCAYCCSEPDAGSDVAGMRTRVTKHGDEYVLNGQKRWITN
                     GGVASWYTVLATFDPAMKHKGIACFVVDASTPGVKPGRKENKMGHRASNTTDVIFEDV
                     KIPKSALIGTEDGGFKIAMKTFDRSRPWIAASATGTIRRALEESRNYALERKTFGVPI
                     AQHQAIQFMLADMAIAYESTRLLTHKAAWLVDKGDLSSIVSAYAKAYGADAAMRCTTD
                     AVQIFGGYGYTKEYPVEKLMRDAKLLQIYEGTSQIQRMVIARNVLGGK"
     gene            complement(1555..>2149)
                     /locus_tag="VFV99_00615"
     CDS             complement(1555..>2149)
                     /locus_tag="VFV99_00615"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5057821.1"
                     /translation="PAAPEIPTVRVAGAEPAPAPAPAVPPPAPVVDPKKSKHAKRDLA
                     PTVAPKPAPIPGLPATKPAPAPTPPPPAPTPTPAVTTPPPVPAPAPAPAPAPAPPAEP
                     KEVEVQMATLSSATVSAIASDHSRQLSQCEGTTELHGDVAVSFQIDGTGKVVKSQLQS
                     SIKNPKVASCILKAVQSWKFPKPPSGSAKGVYSISYQ"
BASE COUNT          315 a          725 c          696 g          413 t
ORIGIN      
        1 cgcactcttc gccgcccagc ttgtcggcat agccatcgcc gcacacttcg cgcgacacac
       61 agtctgcgga gcagccgtcg ccgctcacgg tgtttccgtc gtcgcatcgc tctcctagag
      121 ctagctcgag ctcgccattg ccacagccgg cgggcaagca gacgcccgcg aagcagctgc
      181 ccatcgcgac gctcgacgtc tcgcacgact cgaattccga cttgccctcg caggcggtca
      241 gctgctcggg gtcgacgcag ctgtgatgcg cgagatcgca ggcggtgccc tgcgggcaca
      301 gccgatcgcc gcactctacg agatcgtcat cgatgcaccc gaccagcgcc agggcgagca
      361 gccaccgcat ccacgccgat tatcgacgat tacttcccgc cgagcacgtt acgggcgatc
      421 accatgcgct gaatctgtga tgtgccctcg taaatctgga ggagcttggc atcacgcatc
      481 aatttctcga cgggatactc cttggtgtaa ccgtacccac cgaagatttg cacggcgtcg
      541 gtcgtgcagc gcatggctgc atcggcgccg tacgccttcg cgtaggccga cacgatgctc
      601 gacaggtcgc ccttgtcgac gagccacgcc gccttgtgcg tgagcagccg cgtcgattcg
      661 tacgcgatcg ccatgtcggc gagcatgaac tggatcgcct ggtgctgcgc gatcggcacg
      721 ccgaacgtct tgcgctcgag cgcgtagttg cggctctcct cgagtgcgcg ccggatcgtc
      781 ccggtcgcac tcgccgcgat ccacggccgc gagcgatcga acgtcttcat cgcgatcttg
      841 aagccaccat cctcggtgcc gatcagcgcg ctcttcggga tcttgacgtc ctcgaagatc
      901 acgtcggtgg tgttcgaggc gcggtggccc atcttgttct ctttgcggcc cggcttgacg
      961 cccggcgtgc tcgcgtcgac cacgaagcac gcgatgccct tgtgcttcat cgcggggtcg
     1021 aacgtcgcga gcaccgtgta ccagctcgcg acgccaccgt tcgtgatcca gcgcttctgg
     1081 ccgttcagca cgtactcgtc gccgtgcttc gtcacgcgcg tgcgcatgcc ggcgacgtcg
     1141 ctaccggcat cgggctcgct gcagcagtac gcgcagaaca tcagctccga cgtgagccgg
     1201 ctcaagtact tcttcttctg ctcgtcattg cccgcaatca acagcggcat cgcgccgagc
     1261 gagttcgccg ccatcgacgt gttcacgcca gcgcagccga acgcgacctc ttcttgcacg
     1321 aggcagttgt cgacgcagct gccgccgaga ccgccgtact ccgcggggac ctcgatgttc
     1381 atgaggccgg tctcccaggc cttcttcatc acgtcgcgcg ggaactcgcc ggtctcgtcc
     1441 cagtgacccg ctttgggaat gatctctttg cgcgtgaact cgcgcgcggt ggagaccagg
     1501 gcgttctgtt cttcggtgag gctgaggtcg agtgccatcg ccgggcactc tagctcactg
     1561 gtaggagatg ctgtagacgc ccttcgcgga tcccgacgga ggtttgggga acttccagct
     1621 ctggactgcc ttcagaatgc agctcgcgac cttcgggttc ttgatgctcg actgcagctg
     1681 gctcttcacg accttaccgg tgccgtcgat ctggaacgac acggcgacat caccgtgcag
     1741 ctcggtggtg ccttcgcact gcgacaattg gcgcgagtga tccgaggcaa tcgccgagac
     1801 cgtcgcgctc gacagcgtcg ccatctgcac ctcgacctcc ttgggctcgg cgggcggtgc
     1861 gggcgctggc gcaggtgccg gtgcgggcgc tggaaccggc ggcggcgttg tcaccgcggg
     1921 ggttggggtc ggtgccggcg gcggcggggt cggcgcaggc gccggtttcg tcgctggcag
     1981 ccccggtatc ggcgccggct tgggcgcgac cgtgggggca agatcgcgtt tggcgtgctt
     2041 cgattttttc ggatcgacga cgggcgctgg tggaggcacg gccggtgctg gagccggagc
     2101 cggctcggca cctgccacgc ggacggtcgg aatctcgggc gcggccggt
//
LOCUS       DASUFX010000016         4607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_836326,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000016 DASUFX010000000
VERSION     DASUFX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836326"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1272
                     /gene="clpX"
                     /locus_tag="VFV99_00620"
     CDS             7..1272
                     /gene="clpX"
                     /locus_tag="VFV99_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5057822.1"
                     /translation="MPIEKRDGASLTCSFCGKAQKEVKKLIAGPTVYICDECIGLCND
                     IIAEEVEAEAEQSGNPTILRPSEIKAVLDEYVIGQDRAKKILAVAVHNHYKRIDHKQA
                     GVGPEEEVELQKSNILLLGPTGSGKTLLAQTLARILNVPFAIADATNLTEAGYVGEDV
                     ENIIVSLLQNADHDIERAQRGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIE
                     GTTASVPPKGGRKHPQQEFLQVDTTNILFICGGAFTGIEDIIENRIGQKLIGFGAGMN
                     KKIKRTQWELLQEIEPEDLLKYGMIPEFIGRLPVIAPLHELSEEALVQILTQPKNALV
                     KQYQKLFEMDGVKLKFGKGALTKVANMAASQKSGARGLRAILESALLDIMYDTPGQPV
                     NEVIINEDVIENHASPLITYAKETKTAEA"
     gene            complement(1269..1790)
                     /locus_tag="VFV99_00625"
     CDS             complement(1269..1790)
                     /locus_tag="VFV99_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5057823.1"
                     /translation="MQREEEQIGRPADVDPDSSGAMAVGPDSSGAMAIGSASSGAMAI
                     GPASATRRVLVVDDNDESRRDLVRHFMDAGWQVESARAFRAALGIAVWFSPDVIVSEL
                     QLPDVRGYRFAADYRRAVPHDVTIFAVTRIPPLIFDSARKAGFDDVFGKPIDFDMLER
                     CIETALQTHAQAG"
     gene            complement(1925..2815)
                     /locus_tag="VFV99_00630"
     CDS             complement(1925..2815)
                     /locus_tag="VFV99_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5057824.1"
                     /translation="MIRVALVVALVACHGTDELEKKVTKLEEEIATLRKERQFLEVKH
                     VQLLKEVTELEQKVDELTKKAAPPSFGARPSNRLQPKPDKTYSLAIDPMDSIEGSADA
                     LVTIVEGYEYACPFCERARATMDELEKRYGKDVRWVGKQLVVHPQIATPAALAICAAN
                     LQHKFAAMDHVLWEEGFKARQFDNSGCEADADGCPKMVKFARKLHLDVDRFRTDMKGS
                     CTSWLSGNRRAFEHLGANATPTFFINGRFISGAQPADSFATLIDEELARAKSRVQSGT
                     KPAEYYDKWVVDAGDKDLTP"
     gene            2920..>4607
                     /locus_tag="VFV99_00635"
     CDS             2920..>4607
                     /locus_tag="VFV99_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5057825.1"
                     /translation="MRDDLDSYLAFALAASLAIGAAACDPVKNPGDDEPPGGPVLQKP
                     SRSSTIALSDDGSLVAMVNPEDGSLSVFSTADNTRLSRTATGGSPASVVIAADNTTAY
                     VANRADGTVVRVTGIDGGTPAVDATIQVGAEPARLALSPTGKRLFVAEYAESRVAVID
                     TATNAIRSTIAVDRPRALLVTNNLDDSDDDETLVVAEFFGRPVPGGEARDDGRVGYLR
                     LYSLADLETPKSIKLDSIDSGFAKGGVATNPTVHASPNQLGALAIANDRLYVTSVSAS
                     PEGPTRFDNNVFPVIYVADLAGAAEVRDASGTVNLARKIVDAIPSPSPTSPRFLPGDL
                     SDLDFVPESQVAYAVGRAGDVMVRVTFGTDVQIGSTQNKLIDLAGNDTIGKCQGPTGV
                     AIDGAHQRAYVNCWITHKLGVVDLAAQSMTAALDASPLPTDALEASVQRGMRFYFTGR
                     ARWSNAGANGAKGGEGWSSCGSCHPDGLTDNMTWVFAAGPRQTTSQDGSFSHGAGTQK
                     QRIFNWTGIFDEHHDFERNTRDVSGGLGAITTAATAADCNQLDKEQQVPLTANLL"
BASE COUNT          846 a         1514 c         1470 g          777 t
ORIGIN      
        1 actgtaatgc cgatcgagaa gcgtgacggg gcgtcgttga cctgctcgtt ctgcgggaaa
       61 gcgcagaaag aggtcaagaa gctcatcgcg ggccccaccg tttacatctg tgacgagtgc
      121 atcggcctct gcaacgacat catcgccgaa gaggtcgagg ccgaggccga gcagtccggc
      181 aacccgacca tcctgcgccc gtccgagatc aaggccgtcc tcgacgagta cgtgatcggc
      241 caggaccgcg cgaagaagat cctcgcggtc gcggttcaca accactacaa gcgcatcgat
      301 cacaagcagg ccggcgtcgg ccccgaagag gaagtcgagc tgcagaagtc gaacatcctc
      361 ctgctcggcc ccaccggctc gggcaagacg ctgctcgccc agacgctcgc gcgcattctc
      421 aacgtgccgt tcgcgatcgc ggacgccacg aatctcaccg aggccggcta cgtcggcgag
      481 gacgtcgaga acatcattgt ctcgctgctg cagaacgccg accacgacat cgagcgtgca
      541 cagcgcggca tcgtctacat cgacgagatc gacaagatcg cgcgcaagag cgacaacccg
      601 tcgatcacgc gcgacgtctc cggtgaaggc gtgcagcaag cgctgctcaa gatcatcgaa
      661 ggcacgaccg cgagcgtgcc gcccaagggt ggccgcaagc atccgcagca agagtttctc
      721 caggtcgaca cgacgaacat cctgttcatc tgcggcggtg ccttcaccgg catcgaggac
      781 atcatcgaga accgcatcgg tcaaaagctg atcggtttcg gtgccggcat gaacaagaag
      841 atcaagcgca cgcagtggga gctgctgcag gagatcgagc cggaagacct gctcaagtac
      901 ggcatgattc ccgagttcat cggccgcttg ccggtgatcg cgccgctcca cgagctctcg
      961 gaagaagcgc tggttcagat cctcacgcag ccgaagaacg cgctcgtgaa gcagtaccag
     1021 aagctgttcg agatggacgg cgtgaagctg aagtttggca agggcgcgct gaccaaggtc
     1081 gcgaacatgg cggcgtcgca gaagagcggc gcgcgtggcc tgcgcgcgat cctcgagagc
     1141 gcgctgctcg acatcatgta cgacacgccc ggccagccgg tgaacgaagt gatcatcaac
     1201 gaggacgtga tcgagaatca cgcctcgccg ctcatcacgt acgcgaaaga aacgaagacc
     1261 gccgaagcct aaccggcttg tgcgtgcgtt tgcagcgcgg tctcgatgca gcgctcgagc
     1321 atgtcgaagt cgatcggttt gccgaacacg tcgtcgaagc ccgccttgcg cgcgctgtcg
     1381 aagatcagtg gcgggatgcg cgtgacggcg aagatcgtga cgtcgtgagg gaccgcgcgc
     1441 cgatagtccg cggcgaagcg gtagccgcgc acgtcgggaa gctgtagctc gctgacgatc
     1501 acgtccggcg agaaccagac ggcgatcccg agtgctgctc ggaacgcgcg cgcggactcg
     1561 acctgccagc ccgcatccat gaagtggcgc accagatcgc ggcggctctc gtcgttatcg
     1621 tcgacgacca gcacgcggcg ggttgccgag gccgggccaa tggccatcgc gccgctcgag
     1681 gccgagccaa tggccatcgc gccgctcgaa tccgggccaa cggccatcgc gccgctcgag
     1741 tccgggtcga cgtcagccgg acggccgatc tgttcttcct cgcgctgcac ggtgtcatac
     1801 gactctagca atcgccgtgc gcccgtgaga cctgcttttt gcgcgtaagc ccgtggactg
     1861 gtgatcaaac tgcgcgccca cgccgcgctg tgcgcgtgtg ccacgcggca aatctagcgc
     1921 tgctctacgg agtgaggtct ttgtcgcccg cgtcaacgac ccacttgtcg tagtactccg
     1981 ctggtttggt gccgctctgc acccgcgact tggcgcgtgc aagttcttcg tcgatgagcg
     2041 tcgcgaacga gtcggccggc tgcgctccgg agatgaagcg tccgttgatg aagaacgtcg
     2101 gcgtcgcgtt ggcgcccaga tgctcgaacg ctcgccggtt accgctgagc cacgacgtgc
     2161 acgagccctt catgtcggtg cggaaccgat cgacatcgag gtggagcttg cgcgcgaact
     2221 tcaccatttt cgggcagcca tcggcgtcgg cctcgcaacc gctgttgtcg aactgccgcg
     2281 ctttgaagcc ttcttcccac agcacgtgat ccatcgcagc gaacttgtgt tgcaagttcg
     2341 cggcgcagat cgcgagtgca gccggcgttg cgatctgcgg atgcacgacg agctgcttgc
     2401 cgacccagcg cacgtccttg ccgtaccgct tctcgagctc gtccatcgtc gcgcgtgcgc
     2461 gctcgcaaaa cgggcacgcg tactcgtagc cctcgacgat cgtcacgagc gcgtcagccg
     2521 aaccctcgat cgagtccatc ggatcgatcg cgagcgagta cgttttgtcg ggcttgggct
     2581 gtagccggtt cgatggccgc gcaccgaagc tcggcggcgc cgccttcttc gtcagctcgt
     2641 cgaccttctg ctcgagctcg gtgacctctt tgagcagctg gacgtgtttg acctcgagga
     2701 actgacgctc cttgcgcagc gtcgcgattt cttcctcgag cttggtgact ttcttctcga
     2761 gctcgtcggt tccgtgacaa gccaccagcg ctacgaccaa cgcgaccctg atcacggccg
     2821 cactgtacta catgcggaat tcggctcggc gcgcgtagtt acgcactgaa tgcgacatcg
     2881 cggatcacgc cccctctcgg atccaggagg acaactcgga tgcgtgatga cttggatagt
     2941 tatttggcgt ttgcgctcgc tgcatcgctc gcgatcggtg cagctgcgtg cgatcccgta
     3001 aagaaccctg gcgatgacga gccacccggc ggcccggtgc tgcagaagcc atcgcggtcg
     3061 agcacgatcg cgctgtcgga tgacggctcg ctcgtcgcga tggtgaaccc ggaggacggc
     3121 agcctctcgg tgttctcgac ggcagacaac acacggctgt cgcgcacggc gaccggcggc
     3181 tcgccggcga gtgtcgtgat cgccgccgac aacacgaccg cgtacgtcgc gaatcgcgcg
     3241 gacggcacgg tcgttcgcgt gaccggcatc gacggcggca cacctgcggt cgacgcgacg
     3301 atccaggtcg gtgcggagcc ggcccgactc gcgctatcgc cgaccggcaa gcgcttgttc
     3361 gtagccgaat acgcggagag tcgcgtcgcc gtgatcgata cagcgacgaa tgcgatccgc
     3421 tcgacgattg cagtcgaccg tccgcgcgcg ctgctcgtca cgaacaacct cgatgacagc
     3481 gatgacgacg agacgctggt cgtcgccgaa ttcttcggcc ggccggtgcc gggtggtgag
     3541 gcgcgcgacg atggtcgcgt cggttacctg cgcctgtact cgctcgccga tctcgagacc
     3601 cccaagagca tcaagctcga ttcgatcgat agcggatttg ccaaaggtgg cgtcgcgacg
     3661 aacccgaccg tgcacgcgtc accgaatcag ctcggcgcgc tcgcgatcgc gaacgaccgc
     3721 ctctacgtga cgagtgtgtc ggcgtcgccc gaaggtccga cgcgcttcga caacaacgtg
     3781 ttcccggtca tctatgtcgc cgatctcgcc ggcgccgccg aggtgcgcga tgcgagcggc
     3841 acggtcaacc tcgcgcgcaa gatcgtcgac gcgattccga gcccgtcgcc cacgagccca
     3901 cggtttctgc ccggcgatct cagcgatctc gacttcgtgc ccgagtcgca agtcgcgtac
     3961 gcggtcggtc gcgccggtga cgtgatggtg cgcgtgacgt ttggcaccga cgtccagatc
     4021 ggctcgacgc agaacaagct gatcgatctc gcgggcaacg acacgatcgg caagtgccaa
     4081 ggcccgaccg gcgtcgcgat cgacggtgcg caccagcgcg cgtacgtgaa ctgctggatc
     4141 acgcacaagc tcggtgtcgt cgatctcgcc gcgcagtcga tgacggccgc actcgatgca
     4201 tcaccgctgc cgaccgacgc gctcgaagca tcggtgcaac gcggcatgcg cttctacttc
     4261 acgggccgtg cgcggtggtc gaacgccggc gcgaacggcg cgaaaggcgg cgaaggttgg
     4321 tcgtcgtgtg gctcgtgcca tcccgacggc ctcaccgaca acatgacgtg ggtgttcgca
     4381 gcgggtccgc gccagacgac gtcgcaagac ggctcgttct cgcacggtgc gggcacgcag
     4441 aagcagcgca tcttcaactg gaccggcatc ttcgacgagc atcacgactt cgagcgcaac
     4501 acgcgcgatg tctccggtgg tctcggagcg atcacgaccg cggccacggc ggccgactgc
     4561 aaccagctcg acaaggagca gcaggtgccg ctcaccgcga acctgct
//
LOCUS       DASUFX010000017         6120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1113965, whole genome shotgun sequence.
ACCESSION   DASUFX010000017 DASUFX010000000
VERSION     DASUFX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113965"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1087
                     /locus_tag="VFV99_00640"
     CDS             110..1087
                     /locus_tag="VFV99_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5057826.1"
                     /translation="MGTFTVLGATELGELAEAFSLGAVQSSRTIAAGTINSNFELVTD
                     GGRYFLRINEGKAEVDVAWESRLVAALAEQGVATPPPMLARDGRPYAPLHGGGVPKWV
                     SVFPWRDGSHLAADAITPAIATAFGAELATLHRVGAQLPSAWRRGSIYDHDHLVARFQ
                     RFERLDDPLLAAAKRVLAEEIAVAGAASYIRRRATHGIIHGDLFRDNVLWQGERITAI
                     LDFEQASGGSLAYDLAVCINDWCWTGAPRFELARALIEGYQSVRPLTDSDREALPYEI
                     RAAAMRFTITRITDVYLARVTNPDKDFRAFLARCEAWRGPSLGQLTSTL"
     gene            1134..1628
                     /locus_tag="VFV99_00645"
     CDS             1134..1628
                     /locus_tag="VFV99_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057827.1"
                     /translation="MTRLSVFLAIAALAACGHGGSYVPGTRVPYSEANKSVLQAVEEY
                     RMAVERGDADALMLMAHKQYWEDSGTPSGGDDYGYEGLRNVLTTRLQKASDIRYSMRY
                     IAVHQECDDNLKPGCKAAVDVLIDASFTVTSALGQPKRPDKRDQNQLVLEWDGHRWLF
                     LSGM"
     gene            complement(1629..4178)
                     /locus_tag="VFV99_00650"
     CDS             complement(1629..4178)
                     /locus_tag="VFV99_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057828.1"
                     /translation="MKVVSGRGAIRRMLRRPRLWVWLSLATVVLGFGLGLVPLFNVLG
                     YELAFVMSVFAAISGLDLGAALARELQWMHEPALERAVYPGRALARTSVAAAALAVAV
                     VLPPALIAAVRGIWVPTCDWWFGIKAYLAMPIVTAALAGALGHALGVAVGSRGPSEAR
                     ADRPFAFVFVGTLGAVVGIVVAIAASDVVGGLLVAALVAAVLSLVVWITKPHRSTLVA
                     LVPLLLVAGGALYAFYAAPPVFTYNAILGYFPGNLYDENVQLGMPLLWSRLEQLAWVV
                     AVVAIVAARLDVPRFRVTREPRPASRRIAPLVIALACCAGGVTLHLLGGDLGYRIDAQ
                     EIEAELGGRIETPHFIIHYARTPEIERDIALVAADHEFRYAQVVAQIGVAPPGKIRSF
                     LFASRDQKARWIGAKDVEMAKPWRREIYLEHRGFPHGSLRHEIAHAVASAFGDPMFGV
                     AARRVAGVPVMVSPGLIEGLAVALDWPAGYDRLTPHEAVRAMQAMGLTPSIRELLSLG
                     FLTVSSARSYTTAGSFMRYLLDTYGAPMLRELYRSGGDFDRVYGKSAGALETEWRTMI
                     SSITLPPDEIEGTRERFRAGSVFARPCPHANAARRERAQIANAQGDRPKAIALYRDVC
                     SDAPEEPKYKLELADMLYGGNAAQRAEAIALWTSLSESETVTSTLRVIGFERLARVAS
                     STGDLAKTTAIIERARKLPVDADARRQLDAEWFALHHEGHAGAALRGYFFAPAGTWDQ
                     PSWALLATIAEPDLGFGYYLYGLQKYVAGEWADAADALDKSLTLGVPGISFTRNAARK
                     LAVAAFRANDTARVERAIAVLRGPDMNQSDRLLADDWAQRLAFTATGRLSK"
     gene            4203..4616
                     /locus_tag="VFV99_00655"
     CDS             4203..4616
                     /locus_tag="VFV99_00655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEX5057829.1"
                     /translation="MVDDKAERRTSKRRPIELKVEYKRLNTFFADYTKNISRGGTFIK
                     TSKPLPVGTEFLFKLFVPGRNEPLTIHGEVQRIVSGTDVDGDGDPDEGMAIRFVYREG
                     DPQAEIARVVEGMMTDSLGPRLYAKLMEQAHKRGS"
     gene            4621..5571
                     /locus_tag="VFV99_00660"
     CDS             4621..5571
                     /locus_tag="VFV99_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5057830.1"
                     /translation="MAIPRFFRYLAPNLITLSSMIFGLVSLWAASHGNASLSAWMIIY
                     AVLTDRLDGLVARALKATSELGMQLDSFADFLNFGIAPAFLVLSYVSSRVEYYQHGTG
                     HYLLFIACGAYMLCAVFRLARYNVLSDDQVPTKIFFGFPTTLTGGLIAIWILVFLKYD
                     PTYRTFGGPKLFGDNLQTPESVWKYLPIAMVVGGYLMASRLPMPKVGMTRNKLVSVVL
                     LILVVTGYICGFAMSFPEICFGMPTIWSIMFLIWGQASAKARAMYPPPLFPKASPERK
                     FERPQEDLEDVVLDESRQSQPVLDAAAAAATAAAARHDAK"
     gene            5639..5710
                     /locus_tag="VFV99_00665"
     tRNA            5639..5710
                     /locus_tag="VFV99_00665"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5671..5673,aa:Ala,seq:tgc)
BASE COUNT         1069 a         2099 c         1966 g          986 t
ORIGIN      
        1 agatcatcgc cgcggcctta cgcgagaggt cgagatgact ctccaccaat cgcgcacgct
       61 cggccgcttc cacacttcga ccctatcact ttctgctagc ttccggcccg tgggcacgtt
      121 cacggtgctc ggcgcgaccg agcttggcga gctcgccgaa gcattcagcc tcggcgcggt
      181 tcagtcgtcg cgcacgatcg ctgcgggaac gatcaactcg aacttcgagc tcgtcaccga
      241 cggcggccgt tacttcttgc gcatcaacga aggcaaggcg gaggtcgacg tcgcgtggga
      301 atcgcggctc gtcgccgcac tcgccgaaca aggtgtcgcc acgccaccgc cgatgctcgc
      361 gcgcgacggc cgaccgtacg cgccgctaca cggaggcggc gtcccgaagt gggtgagcgt
      421 gtttccgtgg cgcgatggct cgcatctcgc ggccgatgcg atcacgcccg cgatcgcgac
      481 ggcgttcggc gccgagctcg caacgctgca tcgcgtgggc gcgcagctac cgtcggcgtg
      541 gcggcgcggc agcatctacg atcacgatca tctcgtcgcg cgctttcaac gtttcgagcg
      601 cctggacgat ccgctgctcg ccgcagccaa gcgcgtgctc gccgaggaga tcgcggtcgc
      661 cggtgccgcc tcgtacatcc gccggcgcgc cacgcacggc atcattcacg gcgatctgtt
      721 tcgcgacaac gtgctgtggc aaggcgagcg catcaccgcg atcctcgatt tcgagcaggc
      781 atcgggcggc agcctcgcgt atgacctcgc ggtctgcatc aacgattggt gctggaccgg
      841 cgcacctcgc ttcgagctcg cgcgcgcgct gatcgagggc taccaatcgg tgcgcccgct
      901 gaccgacagc gatcgcgagg cgctgcccta cgagatccgc gcagcagcaa tgcgattcac
      961 gatcacccgc atcacggatg tctatttggc aagggtcaca aaccccgata aggactttcg
     1021 tgcctttctg gcgcgttgcg aagcttggcg tggcccgtcg cttggccaac ttacgtcgac
     1081 gttgtagcgt tccgcgcgct ggctccattt ctgcaaatgc cggcgagtga gcgatgacta
     1141 ggttgtctgt gttcttggcg attgcggctc tcgccgcctg tggccatggc ggctcgtacg
     1201 taccgggcac cagggttcct tattcggaag cgaacaagag cgtgctccag gcggtcgagg
     1261 agtatcgaat ggcagtcgag cgtggtgacg ccgacgcgct gatgctgatg gctcacaagc
     1321 agtactggga ggattccgga acgccgtcgg gcggcgacga ctacggctac gaaggtcttc
     1381 gcaacgtgct gacgacgcgc ctccagaagg cgagcgatat ccgctactcg atgcggtaca
     1441 tcgccgtgca ccaggagtgc gacgacaatc tcaagcccgg ctgcaaggcc gccgtcgacg
     1501 tgctgatcga cgcgagcttc acggtgacga gcgcactcgg ccagcccaag cgccccgaca
     1561 agcgcgatca gaaccagctc gtgctcgagt gggacggcca tcgctggctg ttcctctcgg
     1621 gtatgtagtc acttcgataa gcgtccggtc gcggtaaacg cgagccgttg cgcccaatcg
     1681 tcggcgagca ggcgatcgct ctgattcatg tcggggccgc gcagcacggc gattgctcgc
     1741 tcgacgcgcg ccgtgtcgtt cgcgcgaaac gcggcgacgg ccagcttccg ggcagcgttc
     1801 ctcgtgaagc tgatgccggg cacgccgagc gtgaggctct tgtcgagcgc gtcggcggcg
     1861 tcggcccatt cgccggcgac gtacttctgc aagccgtaga ggtagtagcc aaagccgagg
     1921 tccggctcgg cgatcgtcgc gagcaacgcc cagctcggct gatcccacgt gccggccggc
     1981 gcgaagaagt agccgcgaag ggcggcgccg gcgtgacctt cgtgatgaag cgcgaaccac
     2041 tcggcgtcga gctggcggcg agcgtcggcg tcgacgggca gcttgcgggc acgctcgatg
     2101 atcgcggtcg tcttcgcgag atcgccggtc gacgacgcaa cgcgggcgag tcgctcgaac
     2161 ccgatcacgc gcaacgtcga cgtcacggtc tccgactccg aaagcgaggt ccacagcgcg
     2221 atggcttcgg cgcgctgggc cgcgttgccg ccgtacagca tgtcggcgag ctcgagcttg
     2281 tacttcggct cctcgggcgc gtcgctgcac acgtcgcgat acagcgcgat cgcctttgga
     2341 cgatcaccct gcgcgttcgc gatctgcgcg cgctcgcggc gtgcggcatt cgcgtgcgga
     2401 cacggtcgcg cgaacacgct gccggcgcgg aaccgttcgc gcgtgccttc gatctcgtcg
     2461 gggggcagcg tgatcgagct gatcatcgtg cgccactcgg tctcgagtgc acccgcactc
     2521 ttgccgtaca cgcgatcgaa atcgccgccg ctgcgataca gctcgcgcag catcggcgcg
     2581 ccatacgtgt cgagcagata ccgcatgaac gagccggcgg tcgtatagct gcgcgccgac
     2641 gacacggtga gaaagccgag cgacaacagc tcgcggatcg acggcgtgag gcccatcgcc
     2701 tgcattgcgc gcaccgcttc gtgcggcgtc aggcggtcgt agccggcggg ccagtcgagc
     2761 gcgacggcga ggccttcgat caatccaggg ctcaccatca ccggcacccc ggcgacgcgc
     2821 cgcgcggcga cgccgaacat cggatcgccg aacgcgctcg cgacggcatg tgcgatctcg
     2881 tgtcgcagcg agccgtgcgg aaagccgcgg tgctcgagat agatctcgcg ccgccacggc
     2941 ttcgccatct cgacatcctt cgcgccgatc cagcgcgcct tctgatcgcg actggcgaac
     3001 agaaacgatc ggatcttgcc gggcggcgcg acgccgatct gcgcgacgac ctgcgcatac
     3061 cgaaactcgt gatcggcggc gaccagcgcg atgtcgcgtt cgatctccgg cgtgcgcgcg
     3121 taatgaatga tgaagtgcgg cgtctcgatg cggccgccga gctcggcctc gatctcctgc
     3181 gcgtcgatgc gatagccgag gtcgccgccg agtagatgca gtgtcacgcc gccggcacag
     3241 caggcgaggg cgatcacgag cggcgcaatc cggcggctcg ccggccgtgg ctcgcgcgtc
     3301 acgcgaaacc gcggcacgtc gagccgggcg gcgacgatcg cgacgacagc gaccacccac
     3361 gcgagctgct cgagccgcga ccacagaagc ggcattccga gctggacgtt ctcgtcgtac
     3421 aggttgcccg ggaagtagcc gaggatcgcg ttgtacgtga acacgggcgg cgccgcatag
     3481 aacgcgtaca gcgccccgcc agcgacgagc agcagcggca cgagcgcgac gagtgtgctg
     3541 cgatgcggct tcgtaatcca cacgacgagc gacagcacgg cggcaaccag tgcggcgacg
     3601 agcagcccgc cgacgacgtc gctcgccgcg atcgcgacga cgatcccgac caccgcgcca
     3661 agcgttccga caaacacaaa cgcgaacggc cggtcggcgc gagcctcact cggaccgcgc
     3721 gaccccacgg cgacaccaag cgcatggccg agcgcaccgg ccagcgctgc cgtcacgatc
     3781 ggcatcgcga ggtacgcctt gatcccgaac caccagtcgc aggtcggcac ccagatgccg
     3841 cggaccgcgg caatcaacgc aggcgggagc acgaccgcca ccgccaacgc cgccgccgcg
     3901 acgctggtgc gcgctaacgc acggcccgga tacacagcgc gttcgagcgc aggctcatgc
     3961 atccattgca gctcgcgggc gagtgccgcg ccgaggtcga gcccgctgat cgcggcgaac
     4021 acggacatca cgaacgccag ctcgtagccg agcacgttga acagcgggac cagccccaag
     4081 ccgaacccga gcaccacggt cgccagactg agccagaccc agagtcgcgg ccgccggagc
     4141 atccgccgga tcgcgccccg tccggacacg accttcatca cttaatcctg gtagagtgag
     4201 tcgtggtcga cgacaaggca gagcgacgga cgagcaaacg ccgcccgatc gagctcaagg
     4261 tcgaatacaa gcgactcaac acgttcttcg ccgactacac caagaacatc tcgcggggcg
     4321 gcacgttcat caagacgagc aagccgcttc cggtcggtac ggagttcttg ttcaagctgt
     4381 ttgtccccgg gcgcaacgaa ccgctcacga tccacggtga ggtccaacgc atcgtttccg
     4441 gcaccgacgt cgatggtgac ggtgatcccg atgaaggcat ggcaattcgt tttgtctacc
     4501 gcgagggcga tccgcaagcg gaaatcgctc gcgtcgtaga aggcatgatg accgactcgc
     4561 tcggcccgcg cctctacgcc aagctgatgg aacaggcgca caagcgcggg agctgacggc
     4621 gtggcaattc cgcgcttctt tcgttatctc gcgccgaacc tcatcacgct gtcgtcgatg
     4681 attttcggac tcgtctccct ctgggcggcg agccacggca acgcctcgct gtcggcctgg
     4741 atgatcatct acgcggtcct caccgaccgt ctcgatggcc tcgtcgcccg cgcgttgaaa
     4801 gcgacgagcg agctcggcat gcagctcgac tcgttcgccg acttcctcaa cttcggcatc
     4861 gcgcccgcgt tcctcgtgct ctcgtacgtc agcagccgtg tcgagtacta ccagcacggc
     4921 accggccact acctgctctt catcgcgtgc ggcgcgtaca tgctgtgcgc cgtctttcgc
     4981 ctcgcgcgct acaacgtcct ctccgacgac caagtcccga ccaagatctt cttcggattc
     5041 ccaacgacgc tcaccggcgg cctgatcgcg atctggatcc tcgtcttcct caagtacgac
     5101 ccgacctacc gtacgttcgg tggcccgaag ctgttcggtg acaacctcca gacgcccgag
     5161 tcggtctgga agtacctgcc gatcgcgatg gtcgtcggcg gctacctcat ggcctcgcgc
     5221 ctgccgatgc ccaaggtcgg catgacgagg aacaagctcg tcagtgtcgt cctgctcatc
     5281 ctcgtcgtca ccggctacat ctgcggcttc gcgatgtcgt tcccggagat ctgcttcggg
     5341 atgccgacca tctggagcat catgttcctg atctggggcc aggcctccgc gaaggcacgc
     5401 gcgatgtacc cgccgccgct gttccccaag gcgagccccg agcgcaagtt cgagcgtccg
     5461 caggaggacc tcgaggacgt cgtgctcgac gagtcgcgcc aatcgcagcc ggtcctggac
     5521 gccgcggccg ccgcggcaac cgctgcagct gcgcgacatg acgcgaagtg aggccactcc
     5581 atcggccggc ggcttgacag gtatggatcc ggcgcgctac ccagtgcgca cccaacacgg
     5641 gggtttagct cagcgggaga gcatcggctt tgcaagccga gggtctgggg ttcgatcccc
     5701 caaacctcca cgtagttacg agagcggcgg cggcgttagt caccaattag tcaccggcga
     5761 gtcggcgatc gcggcatgca accggacaat catcgcgcac cggtacggga tatctccgcc
     5821 acgcaactgg gctgtccaac agcagcggtg ccgtgagctc aatcatctgc tctgtgtcgc
     5881 cgccagccgt tcccatcgcg cgccgcagca gtcggaccgc gaggagcaag cctgcccaga
     5941 acgacacgaa cgagactgtg gcgatggggg cgcccggcgg tccgacgacg gcgtcgaagt
     6001 aagcgcagcc agcgcaacgt cgccctggtt cgtgttcgga tgcttgcacg agaaagtcat
     6061 cggttccggc aatcgccaac cacgccggcc actccttctg gatgagccac cgtgcgggta
//
LOCUS       DASUFX010000018        10542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1948016, whole genome shotgun sequence.
ACCESSION   DASUFX010000018 DASUFX010000000
VERSION     DASUFX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948016"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFV99_00670"
     CDS             complement(<1..1452)
                     /locus_tag="VFV99_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5057831.1"
                     /translation="MIALVGCGVGCDGERAQKTTAPRPAHADASTGAGATGGGGNGGS
                     AETPSYLGNLDAPPGTLDALFSALAATESGDPNGRTLLLFFGDSHTAGDAMTSRLRVT
                     WQHQFGDAGRGLVGAGKPPVHHYYQRDVRYGRSGKWTGAVGGHRGDAEPFGVAGLRVS
                     GKSKGAQLWVETCGECQAGTRTAQFEILYYAAPDHGVLRYRVDDGAWQSLPTKTAPIE
                     PSHPGRQVIPLDDGPHKLTLEHGGGGVLDLFGVVLERLKPGVIVDSLGVVGRRLGSLR
                     SWDWAIIGEQLATRDPKLVVLQYGTNEADDPDLVLEDMAKYYDETIQRIRAAVPTAAI
                     LILGPPDMGVREGGKACDRVKKPPRVKDRAGSSSGSGSADVDAGVTPECEWRTPAILR
                     EIIAVEHAAALRNHVAFFDTFAAMGGADQMHGWVIAEPRVAFKDHVHLSNIGYGRWAD
                     ALTGALFEEYGRWRRANGLAPMKSVTPLPSTTPH"
     gene            complement(1469..2848)
                     /locus_tag="VFV99_00675"
     CDS             complement(1469..2848)
                     /locus_tag="VFV99_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5057832.1"
                     /translation="MSAPERSWTIAPALGWFAAALVAGLLVVDGNVSALADRLTKKRE
                     AQSAGPAGTMAAAAQPDGGDDSDLVAKLPTPAAGSHDVAALDDVCIDGTDQACKHWAM
                     DAFYRSFADAKAGKLGRALRVSWYGDSVVATDDLPGRLRSRLQSDLGDGGPGFVFALA
                     PHRFCHNEGITRGGGENWLTYAISMAHTADGFYGPGGASVETNSGKTSMKLAGKITKA
                     ELYYLMQPHGGVATVTADGVEIIHANTKADAKAAAWALGTTDKGASKVEIKGEGKTRV
                     FGISLENATGAVVDNLGIVSVHVKSFAAHNEEHYAAELAHRGADLIMIMIGANEAQWL
                     HPKDHAMKEYQSHYEKLLAPIRKGRPEASCLVVSPTDQAEVKDDGYASRPVMPVLVAA
                     QRAAAHAQGCAFYSTYDWMGGKGSSVKWFKRKYVNSDFLHLSKAGANKMADAVYDALM
                     VGAKQYGPK"
     gene            2934..4274
                     /locus_tag="VFV99_00680"
     CDS             2934..4274
                     /locus_tag="VFV99_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057833.1"
                     /translation="MRTLGSVCLFAAVFAAGCGDDDAVTPDTQTGNHPPPRVIPGGGI
                     GDGAIDGVVNLYVIDNVSHAPISGATVRVGDIDGTTDATGLFIADGVVGPQTVLAKAT
                     GMRSEMWIGANGANMTLALETATAPTPTQANISGSITGFNTLTVPTGHNKTALISYSQ
                     DDKLGDAGNNIQTAGNQNICTTNQPTGGCTFTVTTRTGSVALLAAIYDHDTNGTPLNG
                     NDDTFTLIGWATSSSLNVANGVDQTGIALTMVDVGNLANVTVTFGTPPTSLPNVAAIV
                     GIELGAAGVFQLLPQILTPSAATVLAPKLAAFSGATYRLTAIANNGNAVTSASSAKLV
                     RGQSTMSLDAGTWIDIASSLTLTRSGGSWSPVSGALIQGVEYELPDATQLLSVTSFDG
                     STSFTIPDVLALPASGTLIARGTAMMGTLDLQNFSLDSDFTKVTGFSAQPLQID"
     gene            4289..5761
                     /locus_tag="VFV99_00685"
     CDS             4289..5761
                     /locus_tag="VFV99_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5057834.1"
                     /translation="MRPELDETQTAALDETATQDKSPALDKTATARAKEAPPPAGADL
                     AEGVRLGHFRIERKLGAGGMGEVYLATDLALDRPVAIKVLPSGTASGKARERLIKEAR
                     AQARVNHTNVAHIYFIGEEEGRLYFAMEYVTGKTLAERIAAGPLPVEDALAVIRAATL
                     GLREAQRSGFTHRDVKPSNLMVDGHGVVKVLDFGLAAGSPGSVTDGPVAQTTLAGTPL
                     YMAPEQARGEAIDFRADIYALGATLFHLVSGHPPFIADSVDELLTLHASAARPLVPRG
                     DNPRTTIKAIGSLIARMMAPNPADRFASYDELLRALELASVEQARPAGIWVRGIAAFL
                     DFLLVLIVLGLVEGLVELIHTPGDFVRGPVVLVTTYIYSALAIGRWGTTAGKGLFELQ
                     VVSVTTGDRPTWRQAIRRQLIAATLPLAFTVGDEAFGALHFAWPAFICRILLGLTAVY
                     YFFAMMHSSLRVPGKRAPWDRMSGTMVRYRTTRGSTGAAA"
     gene            5815..6750
                     /locus_tag="VFV99_00690"
     CDS             5815..6750
                     /locus_tag="VFV99_00690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5057835.1"
                     /translation="MALAVSCTPPPPTPMYSAQYAPTPEVQPAAYDPTLAGTVVDRMS
                     DFGFDGGRIDFEMRRDGETILEIARNRYAIPVVMHWSFDELDNLDPAGPITDTTLLPA
                     ADKPNGYGASVVLTTFHIRDMHRPFHRQLEMHARFGDPSARAEPYAYRLPYRAGSTFS
                     VLQGFHGTFSHRGSNEYAIDFDCPVATPVLAARPGVVVASNAAAQGSGTTPEFLEYRR
                     TNFVLVLHDDGTLGEYMHLSPSGVKVSVGQHVERGDELALSGNTGFSSTPHLHFQVMT
                     AASDGISAQSFPFQLAVSAKRTEAPVQGRSYPSWE"
     gene            6787..8229
                     /locus_tag="VFV99_00695"
     CDS             6787..8229
                     /locus_tag="VFV99_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057836.1"
                     /translation="MARLPLAALLGCVLAAPASAQPRPTPATAQSRPAPRRLLEAPVF
                     TIRPQGALSAFVGEGVNVNVAWRAVAEATRYRVTLTAADGTATDVEVRGVVFEKKSLA
                     PGRYQLTVTAIDASGMEGALSEPLPLSVVEVRAIPPGADGPMPPTRGAYAVGTRFSVA
                     GMHCEVTNAAIDDLLVLAENELRVTKPGLATLRCAGIPGYLEKQVVIAPITIAAESTD
                     VPRGAGAIVHVTFASVAYIGEHLDVQALGDITIGDIERTRFGLDVPVAAPLAAKSGGL
                     ELHAGTFEIGRVELRLVAPPAPPPPPARAPLHWQALDVGGQIGAFFPPSTAENATAIG
                     HPLNAADIVTAGPLAGLRLGFFPIARVGLEGEISIAAGGYGDESGIAQILATRAQLAV
                     RVIEAGRVGIRLFAGTGALTTLRARNSSQQSATGEVHGGGALTFALTPNLWLRFQLSD
                     VVTSARDGGYAHVLESQLGIFTRFGRRDSF"
     gene            8245..9126
                     /locus_tag="VFV99_00700"
     CDS             8245..9126
                     /locus_tag="VFV99_00700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5057837.1"
                     /translation="MRCPVCSTRPAEVGVLCEECRDELASPIKISQEQILTRVQTATT
                     AALIDIWGRPHQLDVHSKVGRQVEDHGIAVLEPSVSRLHAELQYSGGAWSVKDLGSAN
                     GTYVEDKLVEGSMPVAAGERIRFGHIAFYLVDDVARLPPAKSARTVTTTIKPQNVPIN
                     KHAQTTDAEFEEESTDVGLPEMRFKLHEPTGGGGGLIEVDGKQVQLTTTQFELMALMI
                     KRMAAEAHQPELVRGFVRSSELIADLSWDTREPSENHVKQLVRRVRRALMKSEIGDLI
                     ESRHRFGYRLRAIPKSP"
     gene            9144..10052
                     /locus_tag="VFV99_00705"
     CDS             9144..10052
                     /locus_tag="VFV99_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX5057838.1"
                     /translation="MRPFVVLTLVMATAAPAAAQKFEYGKYDDVKDVKKIEWLAAAEG
                     GIVLTTGNSETTTATAGLKASRKEGNNKLTLEGSGAYAKAGLWILYDRNGNGTIDDNS
                     ELLAANTITAETLAGKLRYDRFLTELNSLFIAALASRDLPAGKELVFGGQLGYSRSLY
                     KSKTANTVGEFGYDFSREDLVAPGDPVAIHSLRAFIGHHATMTTGTDLDAAFEVLTNL
                     NTLDLPTGKDGGPLQDTRVNTKIAITAKIGQNLAFQTALEAKYDHRPGPLGIKNLAMG
                     FTPEAATLDTLMKASFIYTFVGATPK"
     gene            complement(10057..>10542)
                     /locus_tag="VFV99_00710"
     CDS             complement(10057..>10542)
                     /locus_tag="VFV99_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5057839.1"
                     /translation="KTTRTNRSGRDLRTLLHVPQNDLQSQLASRLERLGFPTLRPAHV
                     QVLNAIAPEGVRLADLVAAVSLPKQTVGDLIDDLETMRMVERFPDPDHGVIKRVRLGP
                     KGRAWAAEVKRVAAATEARWATRLGTKKMKSLRALLEELAATVEDRPSTRTNGARHRR
                     G"
BASE COUNT         1761 a         3695 c         3359 g         1727 t
ORIGIN      
        1 atgcggcgtc gtcgacggta gcggcgtcac cgacttcatc ggcgcgagcc cattcgcgcg
       61 ccgccaccgc ccgtactctt cgaacagcgc accggtcaac gcgtcggccc agcgcccgta
      121 gccgatgttg ctgaggtgca cgtggtcttt gaacgcgacg cgcggctcgg cgatcaccca
      181 gccgtgcatc tgatcggcgc cgcccatcgc agcgaacgtg tcgaagaacg cgacgtggtt
      241 gcgcagcgcg gccgcgtgct cgaccgcgat gatctcgcgc aggatcgccg gcgtgcgcca
      301 ctcgcactcg ggcgtgacgc ccgcatcgac atccgcgctg cccgaaccag agctcgagcc
      361 agcgcgatct ttgacacggg gcggcttctt gacgcgatcg cacgccttgc caccttcgcg
      421 cacgcccatg tcgggcggcc cgaggatcaa gatcgccgct gtcggcaccg ccgcgcgaat
      481 gcgctggatc gtctcgtcgt agtacttcgc catgtcctcg agcacgaggt ccggatcgtc
      541 ggcttcattc gtgccgtact ggaggacgac gagcttcgga tcgcgcgtcg cgagctgctc
      601 cccgatgatc gcccagtccc acgagcgcag gctcccgagc cgacgaccga cgacgccgag
      661 cgaatcgacg atcacaccgg gcttcaggcg ctcgagcacg accccgaaca gatcgagcac
      721 gccgccgccg ccgtgttcca gcgtgagctt gtgcgggcca tcgtcgagcg gaatcacttg
      781 ccggcccgga tgcgacggct cgatcggcgc cgtcttcgtc ggcagtgact gccacgcgcc
      841 gtcgtcgacg cgatagcgga gcacgccgtg atcgggcgcc gcgtagtaaa ggatctcgaa
      901 ctgcgcggtg cgcgttcccg cctggcactc gccgcaggtc tcgacccaca gctgcgcgcc
      961 cttgctcttg cccgacacgc gaaggccggc gacgccaaac ggctcggcat cgccgcgatg
     1021 accgccgacg gctccggtcc acttgccgct gcggccatag cgaacgtcgc gctggtagta
     1081 gtgatgcacc ggtggcttgc ccgccccgac gagcccacgg cccgcatcgc cgaactggtg
     1141 ctgccaggtg acgcgcagcc gcgacgtcat cgcatccccc gcggtgtgcg aatcgccgaa
     1201 gaacaagagc agcgtgcgcc cgttcggatc gccactttcg gtggcagcga gcgccgagaa
     1261 cagcgcatcg agcgtcccgg gtggcgcatc gaggttgccg agatacgacg gcgtctcggc
     1321 gctgccaccg ttgccaccac cgccggtcgc gcctgcgccc gtgctcgcat cggcgtgggc
     1381 tggcctcggt gccgtcgtct tctgcgcgcg ttcgccatcg caaccaacgc cgcaaccgac
     1441 gagcgcgatc aaggatgcaa gcgcgaggct acttcggccc atattgcttc gcgcccacca
     1501 tcaatgcgtc gtacacggcg tcggccatct tgttcgcgcc cgccttcgac aggtgcagga
     1561 agtcgctgtt cacgtacttg cgcttgaacc acttcaccga cgagcccttg ccgcccatcc
     1621 agtcgtacgt cgagtagaac gcgcagccct gtgcatgtgc cgccgcgcgc tgcgccgcga
     1681 cgaggaccgg catcaccggc cgcgacgcgt agccatcatc cttcacctcg gcttgatcgg
     1741 tcggcgacac gacaagacac gacgcctccg gcctgccctt gcgaatcggc gcgagcagct
     1801 tctcgtaatg cgactggtac tccttcatcg cgtggtcttt gggatgtagc cactgtgctt
     1861 cgttcgcgcc gatcatgatc atgatcagat ccgcgccgcg gtgagcgagc tcggccgcgt
     1921 agtgctcctc gttgtgcgcc gcgaagctct tcacgtgcac gctgacgatg ccgaggttgt
     1981 cgacgacagc accggtcgca ttctcgagcg agatgccgaa cacgcgcgtc ttgccctcgc
     2041 ctttgatctc gaccttgctc gcgcccttgt ccgtggtgcc gagtgcccac gctgccgcct
     2101 tcgcatcggc cttcgtgttg gcatggatga tctcgacgcc gtcggcggtc accgtcgcga
     2161 cgccaccgtg cggctgcatc aggtagtaga gctcggcctt cgtgatcttg ccggcgagct
     2221 tcatcgacgt cttgccgctg ttcgtctcga ccgaggcgcc gcccggcccg tagaaaccat
     2281 cggccgtatg cgccatcgag atcgcatagg tcagccagtt ctcgccgccg ccacgcgtga
     2341 tgccctcgtt gtgacagaag cgatgcggtg caagcgcgaa cacgaagcca ggcccgccgt
     2401 cgccgaggtc gctttgcagc cgcgaccgca gtcgcccggg tagatcgtcg gtcgcgacga
     2461 ccgagtcgcc gtaccacgac acgcgcagcg cgcggccgag cttgccggcc ttcgcatccg
     2521 cgaacgagcg gtagaacgcg tccatcgccc agtgcttgca agcctgatcg gtaccgtcga
     2581 tgcagacgtc atcgagcgcg gcgacgtcgt ggctaccagc ggccggcgtc ggcagcttcg
     2641 cgacgaggtc cgaatcgtcg ccaccatcgg gctgtgccgc ggcggccatc gtccccgccg
     2701 gtcctgcact ctgcgcctcg cgcttcttgg tgaggcgatc ggccagcgcg gacacgttgc
     2761 catcgacgac caggaggccc gccaccagcg cggccgcaaa ccaacccaat gccggagcga
     2821 tcgtccagct gcgctcaggc gcggacatcc gggacgaagg tatgcgaacc atggagggag
     2881 tcaatgccgc tggcctaggc ctcgcattgg gatcggacat agggaggatc cgtatgcgta
     2941 cacttggcag cgtttgctta ttcgcggctg tgtttgccgc aggatgcggg gacgacgacg
     3001 ccgtcacacc ggatacccag accggtaatc acccacctcc gcgcgtcatt cctggtggcg
     3061 gaatcggcga cggcgccatc gacggtgtcg tgaatctcta cgtgatcgac aacgtctcgc
     3121 atgccccgat ttccggcgcg accgtgcgag ttggcgacat cgacggcacg acggacgcga
     3181 ccgggttgtt catcgccgac ggcgtggtcg gtccccagac cgtgctcgca aaagcgaccg
     3241 gcatgcgctc cgagatgtgg atcggcgcga acggcgcgaa catgacgctc gcgctcgaga
     3301 ccgcgacggc gccgacaccg acccaggcca acatctccgg ctcgatcacc ggcttcaaca
     3361 cgctgacggt gccgaccggt cacaacaaga ccgctttgat cagctactcg caggacgaca
     3421 agctcggcga cgctggcaac aacatccaga ccgccggcaa ccagaacatc tgcacgacga
     3481 atcagccgac cggcggttgt acgttcaccg tgacgacgcg caccggcagc gtcgcgctgc
     3541 tcgcggcgat ttacgaccac gacacgaacg gcacgccgct caacggcaac gacgacacgt
     3601 tcacgttgat cggctgggcg acgtcgtcga gcctcaacgt cgcgaacggc gtcgatcaga
     3661 ccggcatcgc gctcacgatg gtggacgtcg gcaacctcgc caacgtcacc gtcacgttcg
     3721 gcacgccgcc gaccagccta ccgaacgtcg ccgcgatcgt cggcatcgag ctcggcgcgg
     3781 ccggcgtgtt ccagctgctg ccgcagatcc tcacgccgtc cgcggcgacc gtgctcgcac
     3841 cgaagctcgc ggcattctcc ggcgcgacct accggctcac cgcgatcgcg aacaacggca
     3901 acgccgtaac gtcggcatcg agcgccaagc tcgtgcgcgg ccagagcacg atgtcgctcg
     3961 atgcgggcac gtggatcgat atcgcgtcgt cgctgacgct tacgcgtagt ggtggctcgt
     4021 ggagcccggt cagcggcgcg ctcatccagg gtgtcgagta cgagctgccc gacgcgaccc
     4081 agctgctgtc ggtgacctcg ttcgacggct cgacatcgtt cacgatcccc gacgtcctcg
     4141 cgttgccggc gagcggaacc ctgatcgcgc gcggcactgc gatgatggga acgctggatt
     4201 tgcagaactt ctcgctcgac agcgatttca cgaaggtcac gggcttctcg gcgcagccgc
     4261 tgcaaatcga ttgacgtatg ctcgggtcgt gcggcccgag ctcgacgaga cccaaactgc
     4321 agcgctggac gaaacagcga cgcaggacaa gtcgccggcg ctcgacaaga cagcgacggc
     4381 gcgcgcgaag gaggcgccac cacccgcagg cgccgatctc gccgagggcg tgcgcctcgg
     4441 tcacttcagg atcgagcgca agctgggtgc cggtggcatg ggcgaggtct atctcgcgac
     4501 tgatctcgca ctcgatcggc cggtggcaat caaggtgctg ccgagcggca ccgcgtcggg
     4561 caaggcacgc gagcggctga tcaaagaagc ccgcgcccaa gcgcgcgtga accacacgaa
     4621 cgtcgcgcac atctacttca tcggcgaaga agaaggacgc ctgtacttcg cgatggagta
     4681 cgtcaccggc aagacgctcg ccgagcggat cgcggccggc ccgctccccg tcgaggatgc
     4741 gctagcggtc attcgcgccg ccacactcgg gctgcgcgaa gcgcaacgct ccggcttcac
     4801 gcatcgcgat gtcaaaccgt cgaacctgat ggtcgatggg cacggcgtgg tcaaggtcct
     4861 cgacttcggt ctggctgcag gctcgcccgg cagcgtcacc gacggaccgg tcgcacagac
     4921 cacgctcgcc ggcacgccgc tctacatggc acccgagcaa gcgcgcggcg aggcgatcga
     4981 ctttcgcgcg gacatctacg cgctcggagc gacgctgttc cacctggtct ccggtcaccc
     5041 gccgttcatc gccgacagcg tcgacgagct actgacgttg cacgcgagcg cagcgcgacc
     5101 actcgtgcca cgcggcgaca acccgcgcac gacgatcaag gcgatcggct cgctcatcgc
     5161 gcgcatgatg gcgcccaatc ccgccgaccg cttcgccagc tacgacgagc tgctgcgcgc
     5221 gctcgagctc gcgtcggtcg agcaagcgcg gccggccggc atctgggtac gtggcatcgc
     5281 tgcgttcctc gactttctgc tcgtgttgat cgtcctcggc ctcgtcgagg ggctcgtcga
     5341 gctgatccac accccgggcg acttcgtgcg cggccccgtc gtgctcgtca cgacgtacat
     5401 ctacagcgcg ctcgcgatcg gacgctgggg cacaaccgca ggcaaggggc tgttcgagct
     5461 ccaggtcgtc tcggtcacga ccggcgatcg gccgacctgg cggcaggcga tccggcggca
     5521 gctgatcgcg gcgaccctgc ccctcgcctt cacggtcggc gacgaagcgt tcggagcgct
     5581 ccacttcgcc tggcctgcct tcatctgccg gatccttctc ggcctgacgg ccgtgtacta
     5641 cttcttcgcg atgatgcatt catcgcttcg cgtgcctggc aaacgcgccc cgtgggaccg
     5701 gatgtcgggg acgatggtgc gctatcgcac gacccgcggg agcaccggcg cagccgcctg
     5761 atccgctgct accattgtgc ggtcagcatg cggtggcggc tgttttctcc atgcgtggcc
     5821 ctcgcggtca gctgtacgcc gcctccgccc acgccgatgt attcagcgca gtacgcgccg
     5881 acgcctgaag tccaacccgc tgcctacgat ccgaccctcg ccggcaccgt cgtcgatcgc
     5941 atgagcgact tcggcttcga tggcggccgc atcgacttcg agatgcgccg cgacggcgag
     6001 accatcctcg agatcgcgcg caatcgctac gcgattccgg tcgtcatgca ctggtcgttc
     6061 gacgagctcg acaacctcga tccggcgggg ccgatcaccg atacgacgct gttgcccgcc
     6121 gccgacaagc cgaacggcta cggcgcgtcg gtcgtgctca cgacgttcca catccgcgac
     6181 atgcaccgcc cgtttcatcg ccagctcgag atgcacgcgc gattcggcga tccgagcgcg
     6241 cgtgccgagc cgtacgcgta tcgcctgccc tatcgcgcag gcagcacgtt ctcggtgctg
     6301 cagggctttc acggcacgtt ctcgcatcgc ggctcgaacg agtacgcgat cgacttcgat
     6361 tgcccggtcg cgacgccagt gctcgccgca cgccccggcg tcgtcgtcgc gtcgaatgcg
     6421 gccgcgcagg gctccggcac cacgcccgag ttcctcgagt accgccgcac gaacttcgtg
     6481 ctcgtgctcc acgacgacgg caccctcggc gagtacatgc acctgtcacc gagcggcgtg
     6541 aaggtcagcg tgggacagca cgtcgagcgc ggcgacgagc tcgccctgtc gggcaatacc
     6601 ggcttctcgt cgacgccaca cctgcatttc caggtcatga ccgcggcaag tgatggcatc
     6661 tcggcgcagt ccttcccgtt ccagctcgcg gtctcggcca agcgtacgga ggcgcccgtg
     6721 caaggccgct cgtacccgag ctgggaatag gtcgccggcg cgatgggcgg ctgcgataat
     6781 tcgggcatgg cgcggctccc gctcgccgct ctgctcggct gcgtgctcgc tgcaccagca
     6841 tccgcgcagc cacgcccgac gcctgcaacc gcgcagtcac gcccagcgcc gcgccggctg
     6901 ctcgaggcgc ctgtattcac gatccggccg cagggcgcgc tgtccgcgtt tgtcggtgaa
     6961 ggtgtcaatg tcaacgtcgc gtggcgtgcc gtcgccgagg caacgcgtta tcgcgtgacg
     7021 ctgaccgccg ccgatggcac ggcgaccgat gtcgaggtgc gcggcgtcgt cttcgagaag
     7081 aagagtctcg cgccgggtcg ctatcagctc acagtgaccg cgatcgacgc cagtggcatg
     7141 gagggcgcgc tcagcgagcc gctgccgctc agcgtcgtcg aagttcgcgc gataccgccc
     7201 ggtgccgacg gcccgatgcc accgacacgt ggagcctacg ccgtgggaac gcggttctcg
     7261 gtggctggca tgcactgcga agtcacgaac gcagcaatcg acgacctgct cgtgctcgcg
     7321 gagaacgagc tgcgcgtcac gaagccgggg cttgccacgc tgcgctgcgc gggcatccct
     7381 ggctatctcg agaagcaggt cgtgattgca ccgatcacta tcgccgcaga gtcgaccgac
     7441 gtcccccgcg gtgccggcgc gatcgtccac gtcacgttcg catcggtcgc atacatcggc
     7501 gaacacttgg acgtccaagc attgggggac atcacgatcg gcgatataga gcggaccagg
     7561 ttcgggctcg atgtccctgt cgccgcgccg ctcgctgcga aatccggcgg cctcgagctc
     7621 cacgccggga cgttcgagat cggccgggtc gagctccggc tcgtcgcacc accagcgcca
     7681 ccaccgcctc ctgcccgcgc accactgcac tggcaggcgc tcgatgtcgg tggccagatc
     7741 ggtgcgtttt ttccgccgag tactgccgag aacgcgaccg ccatcggcca cccgttgaat
     7801 gcggcagata tcgtgaccgc cggtccgctc gccggtctcc ggctcggctt tttcccgatc
     7861 gcacgcgtcg ggctcgaggg cgagatctcg attgccgcgg gtggctacgg cgacgagtct
     7921 ggcatcgcgc agatcctggc gactcgcgca cagcttgcgg tccgagttat cgaagcgggc
     7981 cgggtcggaa tacgcctatt cgccggcaca ggcgcgctga cgacgctccg cgcccgcaat
     8041 agttcgcaac aaagcgctac cggggaggtt cacggcggcg gtgcgctgac cttcgcgctg
     8101 acccccaatt tgtggctcag attccaactc tcagacgtgg tgacgagcgc gcgtgacgga
     8161 ggctatgcgc acgttctcga gtcccagctc ggcatcttca cccgattcgg gcgccgcgat
     8221 tccttctagt gatacgatgg ccgggtgagg tgtccggtct gctctacacg ccctgctgag
     8281 gttggggtcc tctgcgagga gtgtcgcgac gagctcgcga gtccgatcaa gatcagtcag
     8341 gagcagatcc tgacgcgcgt ccaaacagcg acgacagccg cgctcatcga tatctggggt
     8401 cgtccccatc agctcgatgt gcactcgaaa gtcggtcgcc aggtcgagga tcacggcatc
     8461 gccgtgctcg aaccgagcgt gtcgcgcttg catgccgagc tgcagtattc cggcggcgca
     8521 tggtcagtga aggatctcgg ctcggcgaac ggcacttatg tcgaggacaa gctcgtcgag
     8581 ggctcgatgc ccgtcgcggc gggcgaacgc atccgcttcg gccatatcgc gttctatctt
     8641 gtcgatgacg ttgcccgtct gccgccggcg aaatcggcgc gtacggtgac gacgacgatc
     8701 aagccgcaga acgtcccgat caacaagcac gcgcagacca ctgacgccga gttcgaggaa
     8761 gagagcacgg acgtcggcct gcccgagatg cggttcaagc tgcacgagcc caccggcggt
     8821 ggcggcggcc tgatcgaggt cgacggcaag caggtccagc tcacgacgac gcagttcgag
     8881 ctgatggcgc tcatgatcaa gcgcatggcg gcggaggccc accagcccga gctcgtgaga
     8941 ggcttcgtac gcagctcgga gctgatcgcc gatctctcct gggatacccg cgaaccgagc
     9001 gagaaccacg taaaacagct cgtccgccgc gttcgccgcg ctctgatgaa gtccgaaatc
     9061 ggtgacctga tcgagtcgcg gcatcgtttc gggtaccgct tgcgcgcgat cccgaagtcg
     9121 ccgtagcgct attctcgggc gcaatgcgcc cattcgtagt gctcaccctg gtgatggcca
     9181 cagctgcgcc cgctgcggcc cagaagttcg aatacggcaa atacgacgac gtcaaagacg
     9241 tcaagaagat cgagtggctc gccgccgccg aaggcggcat cgtgctcacg accggtaact
     9301 cggagacgac gacggccacc gccggcctca aagcgtcgcg caaggagggc aacaacaagc
     9361 tgaccctcga aggcagcggc gcctacgcga aggctggcct ctggatcctc tacgaccgca
     9421 acggcaacgg cacgatcgat gacaacagcg agctgctcgc ggccaacacg atcacggccg
     9481 agacgctcgc aggcaagctg cggtacgacc gcttcctcac cgagctcaac tcgctgttca
     9541 ttgccgcgct cgcgagccgc gacctgccgg cgggcaaaga gctcgtgttc ggcggccagc
     9601 tcggctacag ccgcagcctc tacaagtcaa agacggcgaa caccgtcggc gagttcggct
     9661 acgacttctc gcgcgaggat ctggtcgcgc ccggcgatcc agtcgcgatc cactcactgc
     9721 gcgcgttcat cggccaccat gcgacgatga ccaccggcac cgacctcgat gccgcgttcg
     9781 aggtgctcac gaacctaaac acgctcgacc tgccgaccgg caaggacggc ggcccgttgc
     9841 aggacacccg cgtgaacacg aaaatcgcga tcacggcgaa gatcggtcag aacctcgcgt
     9901 tccagactgc gctcgaggcc aagtacgatc accgcccggg tccgctcggc atcaagaacc
     9961 tcgccatggg cttcacgcca gaagctgcga cactcgacac cctgatgaag gcgtcgttca
    10021 tctacacgtt cgtcggcgcg acgccgaagt gacgcgctag ccgcggcggt gacgcgcgcc
    10081 gttcgtgcgc gtgctcgggc ggtcttcgac ggtggcggca agctcctcga ggagagcgcg
    10141 cagcgacttc atcttcttgg tgcccaggcg ggtcgcccag cgcgcctcgg tggccgctgc
    10201 gacgcgcttg acctcggcgg cccacgccct tccctttggt ccaagccgca cgcgcttgat
    10261 cacgccgtgg tcaggatcgg ggaagcgctc gaccatccgc atggtctcga ggtcatcgat
    10321 caaatcgccc acggtctgct tgggcagcga caccgccgca accagatccg ctagccgcac
    10381 gccttccggc gcgatcgcgt tcaggacctg aacgtgcgcc ggccgcagcg tggggaaccc
    10441 gaggcgctcc agtctgctcg cgagttgcga ctgcagatca ttctggggca cgtgcaatag
    10501 ggtgcgaagg tctcggccgg aacgattggt tcgagtcgtc tt
//
LOCUS       DASUFX010000019        19440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_420012,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000019 DASUFX010000000
VERSION     DASUFX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19440
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420012"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..570
                     /locus_tag="VFV99_00715"
     CDS             235..570
                     /locus_tag="VFV99_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057840.1"
                     /translation="MSYSDFSSWPRDQEQTFVVYEQHATESSKKAVSAGIIGGVLFLM
                     LMIGIYAGVEPDKKDLSKDMNMSNLSKRSTKKVDAPAPAPTPTPAPAPSAAPSETPPA
                     PAAPAEGAK"
     gene            604..1668
                     /locus_tag="VFV99_00720"
     CDS             604..1668
                     /locus_tag="VFV99_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057841.1"
                     /translation="MRTARVLLIVAICACGSRPVAEPKAAEQIAIIAAESGPQGARLV
                     VLDEHGDRRFELVRGADSLARDTHPAVSPNGTWIVFASSRGRSLSETSLWIAPLAADA
                     EPVQLTHGAGIDSHPTWTRDGTAIVFASTREGGDFDLYRLAIDHGHARGEPEQLTHGA
                     GHEVTPTIAGDGSIIYSAVTPVGDGAESHLERRSSDGTITQLTDGPADASPAVSPDGR
                     TVVFARPKEHNGMPDAELWMMPLAGAQAEQLVDLPLTDESGPVWSPDGRFLFATSVLR
                     GAAGNPVFSSVIVIDMRAVPLTARVLNDRVGAVARLTPAVTRVPLDASALASDPEYLP
                     ELVRMMAKAVAAQKADQKNP"
     gene            1665..2543
                     /locus_tag="VFV99_00725"
     CDS             1665..2543
                     /locus_tag="VFV99_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5057842.1"
                     /translation="MTRLALLVAVLAACSSKPDVEHPAAAQLAPLSLAKDQHMVLVPE
                     GQYIAGSTPEERSAAYDDYRDTAGQDTAREKKWFEGEEDRHVVEIPAFRIDLMPVTQV
                     EYAELVAAKLAPPPSIDQAAWQAQGFVQDYSVVERFNWRDGAPPAGREEHPVVLVTWD
                     EARKYCEWRGTLVGETRRLPTEGEYEKAARGDSGIAYPWGNVFEPEKLNSAVKGPGDT
                     TPVGQFVDGKSPYGLLDVAGNVFQWTSTKFKDGHMTVKGSAWEDYGGVGRGASRHGRP
                     VGARHVIVGFRCAAPA"
     gene            2573..3463
                     /locus_tag="VFV99_00730"
     CDS             2573..3463
                     /locus_tag="VFV99_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX5057843.1"
                     /translation="MVDATASAIIVHGGAGEVTADRFARLREGARKAAEAGDAILIAG
                     GSALDAVVAAVRVLEDDPEYNAGTGSALTCDGAVETDASIMDGSTQQVGAVAAVPDLG
                     NAIALARAVLDSEHAILAGPAAWRFAAEHGITPAPAGSLVTERMRTRLATALATKSGG
                     TVGAVARDRAGKYAAATSTGGIVGKRSGRVGDSPIPGAGTWADARCAISATGDGEAIL
                     RVALSRSIAMRIAGGSELRAAITESLRELQTITGGSAGVIAIDDRGVLGVQLSATMPI
                     AHVVRGETIAANDSMGEQIT"
     gene            3836..4711
                     /locus_tag="VFV99_00735"
     CDS             3836..4711
                     /locus_tag="VFV99_00735"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HEX5057844.1"
                     /translation="MEDELAGETADDAALDGKADGAVDGAYTYFEIYRDLRKCSSPSC
                     GGYFLHRLNRTSTVCHNGSTRWSCYVPGLDWTESKLPDELQASLIDAAGRDATAQGVL
                     AIVRGRFAPQSYSGYGNLGRFIVTEAWVAENDAVSEGVFTRVHDNGVRCITAPCPTIG
                     EKGLNTSRTANVHGLDWSVGGFDDDQISKLSTEMVAMPYGILIAGDRYTFKENNVKAK
                     GRTVTAAYRRLVDQTCYVGGCSSQICSDQDGVITTCEWKDEYACYQTATCERQPDGLC
                     GWTPTAELDACLGQN"
     gene            4878..5699
                     /locus_tag="VFV99_00740"
     CDS             4878..5699
                     /locus_tag="VFV99_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057845.1"
                     /translation="MMRAGLLAAVLLFACGPSNRGNGDDDTGGGIDSGNGSGSGSGSG
                     GGEQVFVYAHSPSTLYKIDPDTLQVQMVGAFNWGSVGSDQMTDIAIDKNGQMIGVSFT
                     RVYRVDPSNAATTLLSSQLVRSFNGLSFVPAAQLGMTGDDVLVGTQSTDGAVYRIDPM
                     TGAATQVGNMGAYTSSGDLVGVAGFGTVQTVNGSGSDVLARLATSTFAASPIGTATGF
                     SEIWGVAFFKDKIYGFTNGGAFLLIDPNTGAGTMVSNNGIQWWGAAVTTTAPVIF"
     gene            complement(5950..6963)
                     /locus_tag="VFV99_00745"
     CDS             complement(5950..6963)
                     /locus_tag="VFV99_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5057846.1"
                     /translation="MSYQQIAISKFGSASTLKVSDLTPRAPGDDEVAIDVAYSGVNFA
                     DIQMRLGFYPDAPKKPFVPGYEVSGTVSAVGKGVRDVKPGDAVVAGTYFGGYQSHVTI
                     PARQVFKLPQSVDLAAGAALPVNYFTAQLALFEMARVRKGDRVLIECATGGVGVLAIQ
                     MARHVGAEVTGLTTSPHKKGFIEELGAKAYTRDEFRADTSLRGYDMILNASGGAQINW
                     QRARLGLTGRMVCIGISSGVKDGKRNYLRIATAALQTPRISVLKLFDANTGIYALNAL
                     HVLQDPVWIERLTKAMVGVEEMGLRPHVGKVFPAVEVGAAHAFLETKQATGKVLLQWS
                     GGN"
     gene            complement(6969..7286)
                     /locus_tag="VFV99_00750"
     CDS             complement(6969..7286)
                     /locus_tag="VFV99_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5057847.1"
                     /translation="MPKDDTDRLARMFKALGNPHRLRLFLNLLDESKLDLAKGRIHDC
                     FLVKLLAGIDLGAATVSHHVKELSDAGLIQTQREGKQLICSVDPEGLAHLRDALTVAT
                     SKV"
     gene            complement(7350..7985)
                     /gene="yfcF"
                     /locus_tag="VFV99_00755"
     CDS             complement(7350..7985)
                     /gene="yfcF"
                     /locus_tag="VFV99_00755"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF011693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEX5057848.1"
                     /translation="MADLTLYADQTWESPWAFHAMVALEELRVPYKLEPIRRPIAKDV
                     ADKLREHALLPKVPCLVDGEFWLSESSAISEYLAETFPAPAYPRLLPADPQQRARARQ
                     VMSWLRTSLMGLREDRPTSSVFMRPVTTPLSDKAKANVDELLGVTSRLLVAGRETLFD
                     EWSIADADLALMLMRLVANNDPVPDRVVEYTAAQWGRHSIRRYLAHIPTTS"
     gene            8042..8689
                     /locus_tag="VFV99_00760"
     CDS             8042..8689
                     /locus_tag="VFV99_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057849.1"
                     /translation="MPMFNKSMLVMVLLSATAAQAQPEIDMTGSGDAKPATPTEAVAV
                     PGVAPGGGPWQKGTLGVSFPLTLLSNITSAVSLTAERVPTIDGLYFLDEKTAFDIVVG
                     VLVHKSRYYDNSMPPVAHDQTNFGFALGAGYRMYKHTNKFHTFFEPQAVLAWANVKDK
                     VTLEMSGLAMIGAEAMFTEWSSLAGSVGGGVTANGTFDDIKLATTANLAVNLYWK"
     gene            8703..10772
                     /locus_tag="VFV99_00765"
     CDS             8703..10772
                     /locus_tag="VFV99_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5057850.1"
                     /translation="MVRPALVLLMLARIAHAEGGVEDQGADDADDSGDEEVIEISGRA
                     PREPTQQKLETAELHVLPGAGNDALRGLQSLPGVARVPYGMGGLALRGAAPHDTRVFL
                     DGIEVPILYHFGGLASFVPIDSLDHIELTPSGFGARWGRGIGGVVLLDSRSPKPTSWR
                     AQGEVSLLHAGAMAVGPGPKDGSWIIGVRRSYLDAVLAAAQVDLSLAPSYLDAQVRWE
                     SGDKKWLALAFASGDSLSLVRDPSDDGGAGGVSTSNVKSFDYTSRFVRLGMRYRDRGI
                     TATPWFGLDGIDAIANHKGVDKGYQRFDISGGARVERELVWLGGTLRTGLDAKATHYS
                     YTITNIPPAFPGVPETNVVVTRDGSRAALDAGMFAEQDWLVADKRVTVRPGLRVEYIG
                     LADRVVVDPRLSLIERLDDAVLTQTIGLYHEPPLVTDLDPIYGDRELAAPSSIQVSAS
                     AEAPMFGLFDGRATLYAQAQRSLAVDTVSGATPISDNGGGQSGGLLGISRELVDEQFG
                     SYSYREYVGRGRAFGLELFARKELGALTGWLAYTYARSYRTGDPRVDGRYYPYVLDQP
                     HVLTALATLPLGTKWRIGGRFRLASGNPITPVAGAYVNHKMEWTAVDGPILSQRLPYF
                     AQLDLRIDRLWRRRTAIWNLYLDIQNVTNRLNPEGVTYSDDFSRQSYTRGLPIFPSIG
                     VEYKPVP"
     gene            10823..11917
                     /locus_tag="VFV99_00770"
     CDS             10823..11917
                     /locus_tag="VFV99_00770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057851.1"
                     /translation="MRDSERFGSYTVYECLGAGGMATVHRASIELEDDEIREVALKRL
                     LPQYANDKRFVDDFIREAKLAAELQHPNIVRVLELGRIKGTYFIAMDLVRGRPLVSLM
                     RKAFTKHASAPIGVVLALMTELLDALDHAHDAQIVHRDLTPSNLIVTDDGHLKIIDFG
                     VAKALVGNLQTSSGLAKGKLGYMSSEAISGKQLDTRADIFSAGVVMWELLTTRRLFKG
                     QNEYELIVQIREGLVAPPSHENPECPRELDDIVLQALAKSREDRWSSAGEMRDAIGEL
                     RRFYRASTQDVVRWAQRLRSDALRPEIESEGTEVQISTNDLFAEGSDMETYDEPVELP
                     DEPDPEANRRRFLDSDTVITGESMVDERKR"
     gene            11950..12402
                     /locus_tag="VFV99_00775"
     CDS             11950..12402
                     /locus_tag="VFV99_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057852.1"
                     /translation="MYADANTVRAARAEYFAENGFSDAGYHESWVKVGKLGPIPIAFP
                     SSASRKRAVPLHDLHHVATGYATTWTGEAEIAAWELAGGCGNHLAAWVLNTFAMTYGL
                     VIAPRRIYRAFKRGRKSRTLYHTGWDESLLDLSVGELRQKLGLDRAGA"
     gene            12429..12626
                     /locus_tag="VFV99_00780"
     CDS             12429..12626
                     /locus_tag="VFV99_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057853.1"
                     /translation="MAAPRRKKAAKAPAKAPKPKAGKAKAGAKTGLLASLAAYVAKKA
                     KGLVQRTKKRAKRRAKKAKPA"
     gene            12675..13409
                     /locus_tag="VFV99_00785"
     CDS             12675..13409
                     /locus_tag="VFV99_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057854.1"
                     /translation="MRRALLLVGFAACSSPAAIEVDAAMQPMPDASAADVPANPMGFG
                     DLSGMCGVLAAADLTAPMPMLVRDTFTFARQFVDPTDRPLLTTGGQKLAETPNAGGSS
                     GLSEVFAYEQLARCETAALLKTETEIVYDTTGKITDLEVSLDGHKIGVSVTRAVAYPF
                     GSTYTLTAATTLITRKLSDIQMSTANVSAQDRWDKQILAILAWDGTAADTMAQAWEAL
                     DGNVKADTIVVLTTTDGEDLFIYSNM"
     gene            13464..13700
                     /locus_tag="VFV99_00790"
     CDS             13464..13700
                     /locus_tag="VFV99_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057855.1"
                     /translation="MASDHSIPRLSVSALQELRRIYCKHLLEVFASEETQELYERGCI
                     TGRFSKSPENERATSFIVDSVTPLGVAALELPER"
     gene            complement(13718..14152)
                     /locus_tag="VFV99_00795"
     CDS             complement(13718..14152)
                     /locus_tag="VFV99_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057856.1"
                     /translation="MRGFVLVVLVAACGGHHHGPGHPEDTHHLYVEVAAEGSHRGALR
                     DGAEAGLSKISFATAASDNGDVELQGEVTRLDRAGNTTACSVKILVFRLPQHDLLGIA
                     DGSGHVGGTDGRARDDCIAGVSGTLVRGKVRTLLRRRLDDKR"
     gene            14233..14877
                     /locus_tag="VFV99_00800"
     CDS             14233..14877
                     /locus_tag="VFV99_00800"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5057857.1"
                     /translation="MDWIDISVSIRDGMVHWPDNPPIVVERVMDMAHGDAANVSKISL
                     GVHTGTHVDAPIHFDPKGAAIDKVPLDALCGPARVIAIANPREVTLVELTEANVQGGE
                     RILLRTKNSPAAWKQKTFVPDAVHLTLEAANFLAECEVKTVGIDYLSVGGFAAQNGEE
                     VHHVLLEAGIWIIEGLDLTNAPPGPCELMCLPLKIEGADGAPARAFVRPTRGAR"
     gene            14883..15839
                     /locus_tag="VFV99_00805"
     CDS             14883..15839
                     /locus_tag="VFV99_00805"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX5057858.1"
                     /translation="MRREIGVIGLGRMGGGLAIQAARKGIHVVGYDPLGHHPELQQAG
                     VVPIRALADFVPALATPRAIFLYIPAGPDVDVLLDQLAKLLSRGDVIVDGGNSYWGDS
                     IHRAKRLAALGIELVDAGTSGGIEGARHGACFMVGGSPAGVARVEPLLEELAVPGGYV
                     HAGPPGAGHFTKLVHNGIEFGMLQAIGEGVDLLEHFRDQLDVAGVLACWRNGSVIRGW
                     LIDLMHQQYATAGGMDHIPPFVEDTGEVNWLVADALRMEIAIPVIAQAVMELFASRDN
                     QKNWARAIAMMRNGFGGHPFGPNAHVADERKHGQVDGTFRDE"
     gene            15855..16307
                     /locus_tag="VFV99_00810"
     CDS             15855..16307
                     /locus_tag="VFV99_00810"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5057859.1"
                     /translation="MSTTKSTKRIAAKKSSAAKASAPKRAMPGKRADLGQPIDSFFAK
                     QSPQLRPVLVELRKIIEAVVPDAESSIKWGNPFFTLNGKMMVALSSHKAHVNLILAGP
                     PNAFADPHGRLTGEGKTGRHLKLTSLAELPRDEVRGWVKTAAKIARAK"
     gene            16348..16893
                     /locus_tag="VFV99_00815"
     CDS             16348..16893
                     /locus_tag="VFV99_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057860.1"
                     /translation="MVRVCLLLLLLVACGGDEGNLVASTSFTLSEMSGAGPSPLDPLY
                     QQVIGFDVELVAPSTGYDTADTCKVTAQDSEMPVLTATGATAEMVQNNILNLLPVWSA
                     KLALCEPADQSTATVESDNQGGLAVIIGCLGVPTSALVRDSSGNPKWTDFTATKCQAT
                     VYDQLHGRLFMSRDFSMTIRH"
     gene            17007..17759
                     /locus_tag="VFV99_00820"
     CDS             17007..17759
                     /locus_tag="VFV99_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5057861.1"
                     /translation="MRALRLVAVMCCIAACGGSSGGMNTGDDDGPMADARVFLDAPPT
                     VPPQLMITGTTTERGLSGTTAVAGVTVAVYTNGNDSSSIASAMSDAQGNFSLMVPTNG
                     MPIDGYLLATKASYVDLYMYPTGAFIANSESNDINMITPSNKDFLSNLAGGNQMAGKG
                     LIGLAVVDGQGNPVQGATVSSTPAAGSYHYTGSSGLPDSGATSTSADGVAFMFNVPNG
                     QITVSASKSGMTFKSHVVTARADKMTTTGISE"
     gene            complement(17749..18366)
                     /locus_tag="VFV99_00825"
     CDS             complement(17749..18366)
                     /locus_tag="VFV99_00825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5057862.1"
                     /translation="MTDDEDVPLRVDFRDAATARTWTEETPKKRPYRVNFFTAFCTAL
                     PARSTPPRVPELGSGPGHLAREIVSRCQIASYLALDWAEPMHALAREHLGELAPRVTQ
                     VTRDFRSDTWSEGLGTFDAVVTLQAVHELRHRRHMPRLLGQILPLLVPGGVLLYADHY
                     RTEDSPLPGLSWSRDEQLAVLRAAQFASVERIYEEGSMALYAATR"
     gene            complement(18363..>19440)
                     /locus_tag="VFV99_00830"
     CDS             complement(18363..>19440)
                     /locus_tag="VFV99_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057863.1"
                     /translation="GHISDGLRSVVRMRIGEILLERGLLDWDALATALTDQRPNRMRL
                     CSLLVVRRLLDFDQASQALGEQWHAPAALRRHLVHRDRSVAALLPSAIGKRAVAIPIG
                     RMGDGTLLVCARDPSSQLHDDLARVIGQPIMLAVAPALYLERLVDHVYTEVDVPIDVD
                     EPPPVDFEIEVEEPELDIPVEIEAPEAPKPKNRALPVRIKRLTTETARDSLDETIASF
                     PDIDDLEWLLDVVMGYVAKRWSAALIVAIEDKRAIGLRGHGQRLKPSATRSFILPLSE
                     PSVVQLARDERRIADETPTDAAKRLAVTLDNAAQPTAAPFMTRDTVSHVLVVGDPLAA
                     DHDATIRDLEVLIEAMNEALARIG"
BASE COUNT         3356 a         6151 c         6630 g         3303 t
ORIGIN      
        1 cgcgggtgcg atctgctgcg aagccggctg agcgtcaccc ctcgtactgc aacttgtgga
       61 gatcacgagc aggatcgccc caataaagtg ggaaccgatc catctgtcgg gtgtcatagc
      121 acctcgtgga gggctgtctg gtcctccagt agcaacgaac gagacacacg agcggttgat
      181 tccgttgcag gctttcaaaa taccttctac cgttcgaatc gtacggagga tccgatgagt
      241 tacagcgact tctcttcgtg gccgagggat caagagcaga cgttcgtcgt gtacgaacag
      301 catgccaccg agtcctcgaa gaaggccgtc tcggccggca tcatcggcgg cgtcctcttt
      361 ctcatgctga tgatcggtat ctacgccggc gtcgagcccg ataagaagga cctctcgaag
      421 gacatgaaca tgtccaacct gtcgaagagg tcgacgaaga aggtcgacgc gcctgcaccg
      481 gccccgacgc ccacgcccgc gcccgcgccg tcggccgctc cgtcggaaac gccgccggct
      541 cccgccgcgc ctgctgaagg cgcgaagtaa ggctacagtc cgcgggcgcc gacggcgctc
      601 gagatgagga cagcgcgagt tcttttgatc gtcgcgatct gcgcgtgcgg atcgcggccg
      661 gtcgccgagc cgaaggctgc ggagcagatc gcgatcatcg cggccgagag tgggccgcaa
      721 ggggcacggc tcgtcgtgct cgacgagcat ggcgatcgcc gcttcgagct cgtgcgcggt
      781 gccgattcgc tcgcgcgcga cacccatccg gcggtgtcac cgaacggcac gtggatcgtg
      841 ttcgcatcga gccgcggccg ttcgctctcg gagacgagcc tgtggatcgc gccgctcgcg
      901 gcggatgccg agcccgtgca gctcacgcat ggtgcaggga tcgacagtca tccgacgtgg
      961 acgcgcgatg gaaccgcgat cgtgttcgcg tcgacgcgcg agggtggtga tttcgatctg
     1021 taccggctcg cgatcgatca tggacatgcg cgcggcgagc ccgagcagct cacgcatggc
     1081 gcgggacacg aggtcacgcc gacgatcgcc ggtgacggct cgatcatcta ttcggcggtg
     1141 acgccggtcg gcgacggtgc cgagagtcat ctcgagcgcc ggagcagcga cggcacgatc
     1201 acgcagctca ccgatgggcc ggccgatgcg tctccggccg tgtcgcccga cggccgcacc
     1261 gtcgtgttcg cgcggccgaa ggagcacaac ggaatgcccg acgcggagct atggatgatg
     1321 ccgctcgccg gcgcgcaagc ggaacagctc gtcgatctcc cgcttaccga cgagagtggc
     1381 ccggtgtggt cgcccgacgg tcgcttcctg tttgcgacga gtgtgctgcg cggggctgcg
     1441 ggcaacccgg tattttcatc ggtgatcgtg atcgacatgc gcgcggtgcc gctcacggcg
     1501 cgtgtgctga acgatcgcgt cggtgcggtg gcacggctta ctcccgccgt cacgagagtg
     1561 ccactggacg ctagcgcgct cgcgtccgat ccagagtacc ttcccgagct cgttcgcatg
     1621 atggccaaag cggtggctgc tcagaaggcc gaccagaaga acccatgact cgattggcgc
     1681 ttctcgtcgc tgtactagcc gcgtgctcgt cgaaacccga cgtcgagcat ccagcggctg
     1741 cgcagctcgc gccgctctcg ctcgcgaagg accagcacat ggtgctcgtg cccgagggcc
     1801 agtacatcgc cggctccacg cccgaggaac gatcggccgc gtacgacgat tatcgcgaca
     1861 cggcggggca ggacaccgcg cgcgagaaga agtggtttga aggcgaagag gatcggcacg
     1921 tcgtcgagat tccggcgttc cggatcgatc tgatgccggt gacgcaggtc gagtacgccg
     1981 agctcgtcgc cgcgaagctc gcgccgccgc cgtcgatcga tcaggccgcg tggcaggcgc
     2041 aaggcttcgt gcaggactac tccgtcgtcg agcgcttcaa ctggcgcgac ggcgcaccgc
     2101 cggcggggcg cgaagaacat ccggtcgtgc tcgtcacctg ggacgaagct cgaaagtact
     2161 gcgagtggcg cggcacgctc gtgggtgaaa cgcgcaggct gccgaccgag ggcgagtacg
     2221 agaaagcggc gcgtggtgac agcgggatcg cgtatccgtg gggcaacgtg ttcgagccgg
     2281 agaagctgaa cagcgcagtg aaaggaccgg gcgacacgac gccggtcggc cagttcgtcg
     2341 atggcaagag cccgtatggt ctgctcgacg tcgccggcaa cgtgttccag tggacgtcga
     2401 cgaagttcaa agacgggcac atgacggtca aaggctcggc gtgggaggac tacggcggcg
     2461 tcgggcgcgg tgcatcgcgc cacggtcgcc cggtcggtgc acgccacgtc atcgtcggct
     2521 ttcgctgcgc ggcaccggcg tgaccatggt cgcgggcgcg attggcaggc gcgtggtcga
     2581 cgcaacggcg tcggcgatca tcgtgcacgg cggagccggt gaggtcaccg cggatcggtt
     2641 cgcgcgcttg cgcgagggtg cacgcaaagc cgccgaagca ggcgacgcga tcctgattgc
     2701 aggtggcagc gcgctcgatg cagtggtcgc tgccgtgcgc gtgctcgaag acgatcccga
     2761 atacaacgcc ggaaccggtt cggcgctcac gtgcgatggc gcggtcgaga ccgacgcgtc
     2821 gatcatggac ggctcgacgc aacaggtggg tgcggtcgcg gctgtgcccg atctcggcaa
     2881 tgcgatcgca ctcgcacgcg ccgtgctcga ttccgagcac gcgatcctcg ctggacctgc
     2941 ggcgtggcga tttgctgccg agcacggcat cacgcctgcg ccggcgggct cgctcgtcac
     3001 cgagcgcatg cgcacgcgac tggccacggc gctcgcgacg aaatcaggcg gcaccgtcgg
     3061 tgcggtcgcg cgcgatcgcg ccggcaagta cgctgccgcg acgtcgaccg gcggcatcgt
     3121 cggcaagcga tccggacgcg tcggcgactc gccgattccc ggcgctggca cgtgggccga
     3181 tgcacgttgc gcgatctcgg cgacgggtga tggcgaggca atcctgcgcg tcgcgctctc
     3241 gcgatcgatc gcgatgcgca tcgcgggcgg gagcgagctg cgcgctgcaa tcaccgaatc
     3301 gctgcgcgag ctgcaaacga tcacgggcgg cagtgcgggc gtgatcgcga tcgatgatcg
     3361 cggtgtgctt ggcgtgcaac tgtcagccac gatgccgatt gctcatgtcg tgcgcggcga
     3421 aacgatcgct gcgaacgaca gcatgggcga gcagatcacg tgaatcgcga tcgcgcgtga
     3481 cacgagcgcg cgcgcgttgc gcacaaacgt gaatcgccgc gcggtgaacg gcatgcgatg
     3541 cgtgaaaaca cgcgtctatc gcggcccaca gcgtgcacac ggaacgagcg agcaaaaccg
     3601 atgtgacaag gtgtctcgat cggctgctcg accggccact gcgacagccg tcgatgtcgc
     3661 gggaaaccgc agcggttacg tacatctaga gcgataccta catcggccta cttcgtgctt
     3721 tggccgcgcg gcacattcag ttcgtttgcc tgggagattc ttgaaaatgc ttggttcgct
     3781 taaggccgtt acggccattt ctgttctgtc gattgctgcg tgtgcttcga gtggtgtgga
     3841 agacgagctt gctggggaga ccgcggatga tgccgcgctc gatggcaaag cagatggcgc
     3901 cgtcgatggc gcgtacacgt acttcgagat ctatcgcgat ctccgcaagt gcagctcgcc
     3961 gagctgcggt ggctacttcc ttcatcgctt gaatcgcacg agcaccgttt gtcacaacgg
     4021 ctcgacgcgc tggagctgct acgtccccgg tctggattgg accgagtcga agctgcccga
     4081 cgagctgcag gcgtcgctga tcgacgctgc cggtcgcgat gcgacggcgc agggcgtcct
     4141 cgcgatcgtg cgcggccgct tcgcgccgca gtcgtacagc ggctacggca acctcggccg
     4201 cttcatcgtg accgaggcgt gggtcgccga gaacgacgcg gtttccgagg gcgtgttcac
     4261 gcgcgtccac gacaacggcg tccgctgcat cacggcgccg tgcccgacga tcggtgagaa
     4321 gggtctcaac acgtcgcgca ccgcgaacgt gcacggcctc gactggtcgg ttggcggctt
     4381 cgatgacgat cagatctcga agctctcgac tgaaatggtc gcgatgccgt acggtatcct
     4441 gatcgctggc gatcgctaca cgttcaagga gaacaacgtg aaggcgaagg gccgcaccgt
     4501 cacggccgcg tatcgccgcc tcgtcgatca gacctgctac gtcggtggct gctcgagcca
     4561 gatctgctcg gaccaggacg gcgtgatcac gacgtgcgag tggaaggacg agtacgcctg
     4621 ctaccagacc gcgacctgcg agcgtcagcc cgacggtctc tgcggctgga cgccgacggc
     4681 ggagctcgac gcctgcctcg gccaaaacta agcgcttttc gcttagctat tcgtattccg
     4741 gcgccgccca ccaggggact tggtcctacg ggcggcgtcg gtgttttcgg ccggggacga
     4801 taggccctgg cgaccgatag gtccggccag caacgaacgc tggtcagctc gcgatgccga
     4861 ggagtatggt cgcgccgatg atgagagccg gcctgctggc agcggtactg ctattcgcgt
     4921 gtggtccgtc gaaccgcggc aacggtgatg acgacaccgg cggcggcatc gacagcggca
     4981 acggcagcgg cagtggatcg ggcagcggcg gcggcgagca agtgttcgtc tacgcgcaca
     5041 gcccatcgac gctgtacaag atcgatcccg acacgctgca ggtgcagatg gtcggcgcgt
     5101 tcaactgggg ctcggtcggc tccgaccaga tgaccgacat cgcgatcgac aagaacggtc
     5161 agatgatcgg cgtgtcgttc acgcgcgtgt atcgcgtcga tccgagcaac gcggcgacaa
     5221 cgctgttgtc gagtcagctc gtgcgctcct tcaacggtct atcgttcgtg cccgccgcac
     5281 agctcgggat gaccggcgat gacgtgctcg tggggacgca gagcacagat ggcgccgtgt
     5341 atcgcatcga tccgatgacc ggtgctgcga cccaggtcgg caacatgggc gcctatacgt
     5401 cgagcggcga tctcgtcgga gtcgccggct tcggcaccgt gcagaccgtc aacggctcgg
     5461 gcagcgacgt gctcgcgcgg ctcgcgacgt cgacgtttgc tgcgagtccg atcggcaccg
     5521 cgaccggctt ctcggagatc tggggcgtcg cgttcttcaa ggacaagatc tacggcttca
     5581 cgaacggcgg cgcgttcctg ctcatcgatc cgaacaccgg tgccggcacg atggtgtcga
     5641 acaacggcat ccagtggtgg ggcgccgcag tgacgacgac cgcgcccgtg atcttctagt
     5701 ttccgccgtg ctcgtgcacg tacacgtgca cgtgctcgtg ctcgtgcacg atcaacgctg
     5761 ctccggacgg ctgcccaacc ggacatggcg aaggctcgcg ccttggatcg ttccgcggag
     5821 gcactgtcga tgcgaccgac gctgattggg gacgctgccg agcatcgccc agggaggggc
     5881 accttcccca gtcgctgtgc gagcggatcg tcgacgtgca cgtgcacgtg acacgtgcac
     5941 gtgtacgggt cagtttccgc ccgaccactg caggaggacc ttgccggtcg cctgcttcgt
     6001 ctcgaggaac gcgtgcgccg cgccaacctc gacggcgggg aagaccttgc cgacgtgcgg
     6061 cctcaggccc atctcctcga caccgaccat cgccttcgtg aggcgctcga tccacacggg
     6121 gtcttgcagc acgtggagcg cgttgagcgc gtagatgccg gtgttcgcgt cgaacagctt
     6181 gagcacgctg atccgcggtg tttgcagcgc ggcggtcgcg atgcggagat agttgcgctt
     6241 gccgtccttc acacccgacg agatgccgat gcacaccatg cggccggtga ggccgaggcg
     6301 cgcgcgctgc cagttgatct gcgcgccacc cgatgcgttg aggatcatgt cgtagccgcg
     6361 caggctcgtg tcggcgcgga actcgtcgcg cgtgtatgcc ttggcaccga gctcctcgat
     6421 gaagcccttc ttgtgcgggc tcgtcgtcag gcccgtgacc tcggcgccga cgtggcgcgc
     6481 catctggatc gcgaggacgc ccacgccgcc ggtcgcgcac tcgatgagca cgcgatcgcc
     6541 tttgcgcacg cgcgccatct cgaatagcgc gagctgcgcg gtgaagtagt tgacgggcag
     6601 ggcggcacct gcggcgaggt cgaccgactg cggcagcttg aacacctggc gcgctggaat
     6661 cgtgacgtgc gactggtagc cgccgaagta cgtgccggcg acgaccgcgt cgcctggctt
     6721 cacgtcgcgg acacctttgc cgaccgcgct gaccgtaccg ctgacttcgt agccgggcac
     6781 gaacggcttc ttcggcgcgt ccggatagaa gccgagccgc atctggatgt cggcgaagtt
     6841 gacgccggag tacgcgacgt cgatcgcgac ttcatcgtcg ccgggggcgc gtggcgtgag
     6901 gtccgagacc ttgagggtgg aggcagaacc gaacttcgag atcgcgattt gttggtagct
     6961 catagtttct aaactttcga agtggcaacg gtgagtgcgt cgcgtagatg ggcgaggcct
     7021 tccgggtcga ccgagcagat gagctgcttg ccttcgcgct gcgtctggat caggcccgcg
     7081 tcggataact ccttgacgtg gtgcgagacc gtcgcggcac cgaggtcgat gccggcgagc
     7141 agtttgacga ggaaacagtc gtgaatccgg cctttcgcca ggtcgagctt gctctcgtcg
     7201 agcaggttca agaacaggcg caggcgatgc ggattgccga gcgctttgaa cattcgcgcg
     7261 aggcggtcgg tgtcgtcctt gggcacatcg actcagtacg aagtttatcg aactgtgtca
     7321 acggtcattt tgaagactat cgaactattt caggacgtgg tggggatgtg cgcgagatac
     7381 ctgcgaatcg agtgacggcc ccactgcgcg gccgtgtact cgacgactcg gtctggcacc
     7441 ggatcgttgt tcgcgacgag ccgcatcagc atgagcgcga ggtcggcatc ggcgatcgac
     7501 cactcgtcga acagtgtctc gcgtcccgcg acgaggagcc gcgacgtcac gccgagtagc
     7561 tcgtcgacgt tggccttcgc cttgtccgac agcggcgtcg tgactgggcg catgaacacc
     7621 gacgacgtcg gacgatcctc gcgcagaccc atgaggctcg tgcgcagcca cgacatcacc
     7681 tgccgcgcac gtgcgcgctg ctgcggatcg gcgggcaaga ggcgcggata ggccggcgcc
     7741 gggaatgtct cggcgaggta ctctgaaatc gccgatgatt cgctcagcca gaactcgccg
     7801 tcgacgagac acggcacctt cggcagtagc gcgtgctcgc gcagcttgtc ggcgacgtcc
     7861 ttcgcgatcg gccggcggat cggctcgagc ttgtacggca cgcgcagttc ttcgagcgcg
     7921 accatcgcgt ggaacgccca cgggctctcc cacgtttggt ctgcgtacag cgtcaggtca
     7981 gccacggcgg tctccaaaag tttgcccgcg ctaatgcggg cgctcgatga tccgtcgggg
     8041 gatgccgatg ttcaacaagt cgatgctcgt gatggtgctt ctctcggcga cggccgctca
     8101 ggcgcagccc gagatcgata tgaccggctc tggcgacgca aaacctgcga cgccgaccga
     8161 agcggtcgcg gtgccgggcg tcgcgccggg cggcgggccc tggcagaagg gcacgctcgg
     8221 cgtgtcgttt ccactgacgc tgctgtcgaa catcacgagc gcggtgtcgc ttacggccga
     8281 gcgcgtgccg acgatcgacg gcctgtactt cctcgacgag aagaccgcgt tcgacatcgt
     8341 cgtcggcgtg ctcgtccaca agtcgcgcta ctacgacaac tcgatgccgc cggttgcaca
     8401 cgaccagacg aacttcgggt tcgcgctcgg cgccggctac cggatgtaca agcacacgaa
     8461 caagttccac acgttcttcg agccgcaggc ggtactcgcg tgggcgaacg tgaaggacaa
     8521 ggtcacgctc gagatgtcgg gcctcgcgat gatcggcgcc gaggcgatgt tcaccgagtg
     8581 gtcgtcgctg gcaggctcgg ttggcggcgg cgtcacagca aatggcacgt tcgacgacat
     8641 aaagctcgcg acgacggcca acctggccgt gaatctgtac tggaagtaga tacagtcggc
     8701 gcgtggtccg tcccgcgctc gtgctgttaa tgctcgcgcg gatcgcgcac gccgagggtg
     8761 gcgtcgagga tcagggcgcg gacgacgcgg acgacagcgg cgacgaagag gtcatcgaga
     8821 tctcgggccg cgcaccgcgc gagccgacac aacagaagct ggagacggcg gagctccacg
     8881 tattgcccgg cgccggcaac gatgcgttgc gcggcctgca gagtctgccc ggtgtcgcgc
     8941 gcgtgccgta tggcatgggc gggctcgcgc tgcgtggcgc agcgccgcac gacacgcgcg
     9001 tgttcctcga cggaatcgag gtgccgattc tgtaccactt cggcggcctc gcctcgttcg
     9061 tgccgatcga ttcgctcgat cacatcgagc tcacgccgag cggctttggc gcgcgctggg
     9121 gccgcggcat cggtggtgtc gtgctgctcg attcgcgctc gccgaaaccc acgtcgtggc
     9181 gcgcgcaggg cgaagtgagc ctgttgcacg cgggcgcgat ggccgtcgga cccggcccga
     9241 aagatggcag ctggatcatc ggcgtgcgtc gctcgtatct cgatgccgtg ctcgcggccg
     9301 cgcaggtcga tctatcgctc gcgccgagct acctcgacgc gcaggtgcgc tgggaatccg
     9361 gcgacaagaa gtggctcgca ctcgcgttcg cgtcggggga ctcgctatcg ctcgtgcgcg
     9421 atccgagcga tgacggcggc gccggtggcg tctcgacgtc gaacgtgaag agcttcgatt
     9481 acacgtcgcg gttcgtgcgg ctcggcatgc gttatcgcga tcgcggcatc acggcgacac
     9541 cgtggtttgg actcgacggc atcgatgcaa tcgcgaacca caagggcgtc gacaagggct
     9601 accagcgctt cgacatcagc ggcggtgcgc gcgtcgagcg cgagctcgtg tggctcggcg
     9661 gcactctgcg gaccggcctc gacgcgaaag cgacgcacta cagctacacg atcacgaaca
     9721 tcccgccggc gttcccgggc gtgccggaga cgaacgtcgt cgtcacgcgg gatggctcgc
     9781 gcgccgcgct cgatgcgggc atgttcgccg agcaagattg gctcgtcgcc gacaagcgtg
     9841 tgacggtgcg tcccggcctg cgcgtcgagt acatcgggct cgccgatcgc gtcgtcgtcg
     9901 atccgcggct cagtctgatc gaacggctcg acgacgccgt gctcacgcaa acgatcggcc
     9961 tctatcacga gccaccgctc gtcaccgatc tcgatccgat ctacggcgat cgcgagctcg
    10021 cagccccgag ctcgatccag gtgtcggcga gcgccgaggc accgatgttc ggtttgttcg
    10081 acggccgcgc gacgctctac gcgcaggcgc agcgctccct cgcggtcgac acggtgtcgg
    10141 gcgcaacacc gatctccgac aacggaggcg ggcagagcgg cggcttgctc ggcatcagcc
    10201 gcgagctcgt cgacgaacag ttcggctcgt acagctatcg cgagtacgtc ggccgcggcc
    10261 gtgcgttcgg cctcgagctg ttcgcacgca aagagctcgg cgcgctgacc ggctggctcg
    10321 cgtacacgta cgcgcgctcg tatcgcaccg gcgatccgcg cgtcgacggt cgctactatc
    10381 cgtacgtcct cgatcagccc cacgtgctga ccgcactcgc gacgctgccg ctcggcacga
    10441 agtggcgcat cggcggccgg tttcgtcttg cgagcggcaa cccgatcacg ccggtcgccg
    10501 gcgcgtacgt gaaccacaag atggaatgga ccgctgtgga cgggccgatc ctgtcgcagc
    10561 ggctgccgta cttcgcccag ctcgacctcc ggatcgatcg actgtggcgc cgccgcacag
    10621 cgatctggaa tctatatcta gatattcaaa acgtgacaaa ccggctaaat cccgagggag
    10681 tcacgtattc cgacgacttt tcccggcaga gctacacgcg aggcttgccg atctttccgt
    10741 cgatcggtgt tgaatacaaa ccagtgccgt agctagacct acatcgcgag gtcacgcaaa
    10801 acctgcatac gatagagcag tcgtgcggga cagcgagaga ttcggctcgt acaccgtgta
    10861 cgagtgcttg ggcgccggag gcatggcgac cgtgcaccgc gcgtcgatcg agctcgaaga
    10921 cgacgagatc cgcgaggtcg cgctcaagcg cctgttgccg cagtatgcga acgacaagcg
    10981 cttcgtcgac gacttcatcc gcgaagcgaa gcttgccgct gaattgcagc acccgaacat
    11041 cgtgcgcgtg ctggagctcg gtcggatcaa aggtacgtac ttcatcgcga tggatctcgt
    11101 gcgcgggcgg ccactcgtct cgctcatgcg caaggcgttc acgaagcatg catcggcacc
    11161 gatcggtgtc gtgctcgcgc tgatgaccga gctgctcgac gcgctcgatc acgcgcacga
    11221 cgctcagatc gtgcatcgcg atctgacgcc gtcgaatctg atcgtcaccg acgacggtca
    11281 tctcaagatc atcgacttcg gtgtcgcgaa ggcgctcgtt ggcaatctgc agacgagctc
    11341 ggggctcgcg aagggcaagc tcggctacat gtcgagcgaa gcgatcagcg gcaagcagct
    11401 cgacacgcgc gctgacatct tctctgcggg cgtcgtgatg tgggagttgc tcacgacgcg
    11461 gcggctgttc aagggccaga acgagtacga gctgatcgtg cagatccgcg aggggctcgt
    11521 cgcgccgccg tcgcacgaga atcccgagtg cccgcgcgag ctcgacgaca tcgtcctgca
    11581 ggcgctcgcg aagtcgcgcg aggatcgctg gtcgtcggcg ggcgagatgc gcgacgcgat
    11641 cggcgagctg cggcggttct atcgggcgag cacgcaggac gtcgtgcgct gggcccagcg
    11701 cctgcgttcg gatgcgctgc gtcccgagat cgagtccgag ggcaccgagg tccagatctc
    11761 gacgaacgac ttgttcgccg agggctcgga catggagacg tatgacgagc cggtcgagct
    11821 gcccgacgag cctgaccctg aggcaaaccg tcgcaggttc ctcgattccg atacggtaat
    11881 cacaggcgaa tcgatggtcg acgagcgcaa acggtgagac cgggcagcgg cggaccgcat
    11941 cgaaccgtca tgtacgcgga tgccaacacg gtgcgcgcag cacgcgcgga gtacttcgcg
    12001 gagaacggtt tctccgacgc cggctaccac gagagctggg tcaaggtcgg caagctcggg
    12061 ccgatcccga tcgcatttcc gagctcggcg tcccgcaagc gcgcagtgcc gctacatgac
    12121 ctgcatcacg tcgccaccgg ctacgcgacg acatggaccg gcgaggccga aatcgcagcg
    12181 tgggagctcg cgggcggctg cggcaaccac ctcgcagcgt gggtgctcaa tacgttcgcg
    12241 atgacctacg ggctcgtcat tgccccgcgc cgcatctatc gcgcgttcaa gcgtggtcgc
    12301 aagagccgca cgctctatca cacgggctgg gacgagtcgc tgctcgacct gtcggttggc
    12361 gagctacgcc aaaagctcgg cctcgatcgc gccggtgcgt agatcgcgat gctgatatga
    12421 tcgctgcaat ggcggcacca cgacggaaga aagcagccaa ggcgcccgca aaagcgccga
    12481 agccaaaagc tgggaaagcg aaggcgggcg cgaagaccgg tctgctcgcg tcgctcgcgg
    12541 cgtatgtcgc gaagaaggcc aagggtctcg tccagcgcac gaagaagcgc gccaagcgcc
    12601 gagcgaagaa ggcaaaaccc gcctgagcgt ttttgatggt gcgcggcgcg gactttgact
    12661 aaggtccgcg ccccatgcgc cgtgcgttgc tgttggttgg atttgctgcg tgcagctcgc
    12721 ccgcagcgat cgaggtggat gcggcgatgc agccgatgcc cgatgcgagc gccgccgatg
    12781 tgcccgcgaa tccgatgggc ttcggtgacc tatccggcat gtgcggcgtg ctggccgccg
    12841 ccgacctcac cgcgccaatg ccgatgctcg tgcgcgatac gttcacgttc gcgcgccagt
    12901 tcgtcgaccc gacggatcgg ccgctgttga ccacgggcgg ccagaagctc gccgagacgc
    12961 cgaacgccgg tggtagctcg ggcctgtcgg aggtgttcgc gtacgagcag ctcgctcgct
    13021 gtgagaccgc ggcgctgctc aagaccgaga ccgagatcgt ctacgacacg acggggaaga
    13081 tcaccgacct cgaggtctcc ctcgacgggc acaagatcgg cgtcagcgtc acgcgcgccg
    13141 tcgcctatcc gttcggctcg acgtacacgc tcacggctgc caccacgctg atcacgcgca
    13201 agctgagcga catccagatg tcgaccgcga acgtgtccgc gcaagaccgt tgggacaagc
    13261 agatcctcgc gatcctggcc tgggacggga cggccgcgga cacgatggcg caggcgtggg
    13321 aggcgctcga cgggaatgtc aaggccgaca ccattgtcgt gctgacgacg accgacggcg
    13381 aagacctgtt catctattcg aatatgtagt cgccggccgg gcgcctgccg taggatgtca
    13441 gccgggtgag cgacactatg cttatggcca gcgatcattc gatcccacgc ctgtctgtgt
    13501 ccgcgctgca ggagctgcgg cgtatctact gcaagcatct gctcgaggtg tttgcatcgg
    13561 aagagacgca ggagctttac gagcgcggct gcatcaccgg acgcttctcg aagagcccgg
    13621 agaacgaacg cgcgacgagc ttcatcgtcg attcggtcac gccgctcggc gttgctgcgc
    13681 tcgagctgcc agaacgctaa gtccgcgcgg tgggttctta gcgcttgtcg tcgagccggc
    13741 ggcgcaacaa ggtgcggact ttgccgcgta cgagcgtgcc gctgacgccc gcaatgcagt
    13801 catcgcgcgc gcggccatcg gtgccgccga cgtgtcccga gccgtcggcg atgccgagca
    13861 gatcgtgttg tggcaaccgg aacacgagga tcttgacgct gcacgcggtc gtgttgccgg
    13921 cgcgatcgag gcgcgtgact tcgccttgca gctcgacatc tccgttgtcg ctcgccgcgg
    13981 tcgcgaacga gatcttcgac aggccagcct cggcgccatc gcgcagtgca ccgcgatggg
    14041 agccctccgc tgcgacctcg acgtatagat gatgcgtgtc ctcgggatgg ccgggaccat
    14101 ggtgatgacc accacacgca gcgacgagca cgacgaggac aaagccgcgc acgtagtcga
    14161 tcgtactagg cgccgccagc gaactcttca ctccggacga gtcttttagg cgctccgatc
    14221 cagagttccg atgtggactg gatcgatatc agtgtctcga ttcgcgacgg catggtgcac
    14281 tggccggaca acccaccgat cgtcgtcgag cgcgtgatgg acatggcgca cggcgacgcg
    14341 gccaacgtgt cgaagatctc gctcggcgtg cacaccggca cgcacgtcga tgcgccgatt
    14401 cacttcgatc caaagggagc ggccatcgac aaagttccgc tcgacgcgtt gtgcggaccg
    14461 gcgcgcgtga tcgcgatcgc gaatccacgc gaggtcacac ttgtcgagct caccgaagcg
    14521 aacgtgcagg gcggcgagcg catcctgctg cgcacgaaga attcacccgc tgcatggaag
    14581 cagaagacgt tcgtgcccga tgccgtgcac ctgacgctcg aggccgcgaa ctttctcgcc
    14641 gagtgcgagg tgaaaacggt cggcatcgat tacctctccg ttggtggctt cgccgcccag
    14701 aacggtgagg aagtccatca cgtgctgctc gaagcgggca tctggatcat cgagggactc
    14761 gatctgacaa atgcaccacc aggtccatgc gagctgatgt gcttgccgct caagatcgag
    14821 ggagccgacg gcgcgccagc gcgcgcgttc gtgcgcccga cccgaggtgc gcgatgagcg
    14881 acgtgcgtcg cgagattggc gtgattggac ttggccgcat gggcggcggc ctcgcgatcc
    14941 aggcggcacg caaaggtatc cacgtcgtcg gctacgatcc gctcggccac caccccgagc
    15001 tgcagcaagc aggcgtggtg ccgattcggg cgctcgctga ctttgtccct gcgctcgcga
    15061 cgccgcgggc aatcttcctc tacatcccgg ccgggcctga cgtcgatgtg ctcctcgatc
    15121 agcttgcgaa gttgctgtcg cgcggcgacg tgatcgtcga cggtggtaac tcgtattggg
    15181 gcgactcgat ccatcgcgcc aagcggctcg ctgcgctcgg gatcgagctc gtcgacgccg
    15241 gcacgtcagg cggcatcgaa ggcgcgcgcc acggcgcgtg cttcatggtc ggcggttcac
    15301 ccgcgggagt cgcgcgcgtc gagccgctgc tcgaggagct cgcagttccc ggcggttacg
    15361 ttcatgccgg cccaccgggt gccgggcact tcacgaagct cgtgcacaac ggcatcgagt
    15421 tcggcatgct ccaggcgatt ggcgagggtg tcgacttgct cgaacacttc cgcgatcaac
    15481 tcgacgttgc tggcgtgctc gcgtgctggc gcaatggctc ggtgattcgc ggatggttga
    15541 tcgatctgat gcaccagcag tacgcgaccg cgggtggcat ggatcacatc ccgccgttcg
    15601 tcgaggacac cggcgaggtc aactggctcg tcgccgacgc gctgcgaatg gaaatcgcga
    15661 tacccgtcat cgcgcaagcc gtgatggagc tgttcgcatc acgcgataat cagaagaatt
    15721 gggcgcgtgc catcgcgatg atgcgaaacg gattcggtgg tcacccgttc gggccaaatg
    15781 cgcatgtcgc cgacgagcgc aagcatggtc aagttgacgg tacgtttcgc gacgaatagt
    15841 gcatagtccg gctcgtgtcg actacgaagt ccacgaagcg aatcgccgcg aagaagtcat
    15901 ccgccgcgaa ggcgtctgca ccgaagcgcg cgatgccggg aaagcgtgcc gacctcggtc
    15961 agccgatcga cagcttcttc gccaagcagt cgccgcagct gcgacccgtg ctggtcgagc
    16021 tccgcaaaat tatcgaggcc gtggtccccg acgcggagtc gtcgatcaag tggggcaatc
    16081 cgttcttcac gctgaacggc aagatgatgg tcgcgttgag cagccacaaa gcacacgtca
    16141 atttgatcct cgccggcccg ccgaacgcgt tcgccgatcc acacggccga ctcacgggcg
    16201 aaggcaagac gggccgccac ctcaagctga cgtcgctcgc cgagctgccg cgtgatgaag
    16261 tgcgcggctg ggtaaagaca gccgcaaaga tcgcgcgcgc gaagtaggtc acgctcgcgc
    16321 cggcgcgaca tcctctatga tgggggcatg gtccgcgttt gcttgttgtt gttgctgctc
    16381 gtcgcctgtg gcggtgacga gggcaacctc gtcgcatcga cgagcttcac gctgtccgaa
    16441 atgagcgggg cgggcccgtc gccgctcgat ccgctgtatc agcaagtcat cgggttcgac
    16501 gtcgagcttg tcgcaccgtc gacgggttac gacacggccg atacctgcaa ggtgaccgcg
    16561 caggacagcg agatgccggt gctcacggcg accggcgcga ccgccgagat ggtgcaaaac
    16621 aacatcctga acctgctgcc cgtgtggagc gcgaagctcg cgctctgcga gcccgcggat
    16681 cagtcgacgg cgacggtcga gtccgacaac cagggcggcc tcgccgtgat catcgggtgt
    16741 ctcggcgtgc cgacgagcgc gctggtgcgc gacagcagcg ggaacccgaa gtggacggac
    16801 tttacggcga cgaagtgcca ggcgacggtc tacgatcagc ttcacggccg cctgttcatg
    16861 agccgcgatt tctcgatgac gatccggcac tagttgcgca gtgctctgcg ctttgtcgtt
    16921 gcgcagtgca ctgcgcgtcg agtgtcgacg gaaacgttag cgcgtgacag tcggcgccct
    16981 cgacgaagtc atgcagcatg ccgcgcatga gagcacttcg attggttgcg gtgatgtgct
    17041 gcatcgccgc gtgtggcggc agcagcggtg gcatgaacac cggcgatgac gacggcccga
    17101 tggccgacgc gcgagtgttc ctcgatgcgc cgccgacggt tccgccgcaa ttgatgatta
    17161 ccggcaccac gacggagcgc gggctcagcg gcacgaccgc agtcgccggg gtgaccgttg
    17221 ccgtctacac gaatggcaac gactcgtcgt cgatcgcgtc ggcgatgagc gacgcgcagg
    17281 gcaacttctc gctgatggtg ccgacgaacg gcatgccgat cgatggctat ctactcgcga
    17341 cgaaagcgag ctacgtcgac ctgtacatgt atccgaccgg ggcgttcatc gcgaacagcg
    17401 agagcaacga catcaacatg atcacgccga gcaacaagga cttcttgagc aatcttgccg
    17461 gcggcaatca gatggccggc aaagggctga tcggtctcgc tgtcgtcgac gggcagggta
    17521 acccggtgca gggcgcgact gtttcgtcga cgcccgccgc cggctcgtat cactacaccg
    17581 gctcgagtgg actgccggac agcggtgcga cgtcgacgag cgcggacggc gtcgcgttca
    17641 tgttcaacgt gccgaacggc cagatcacgg tgagtgcgag caagagtggc atgacgttca
    17701 agtcgcacgt cgtgactgcc cgcgccgaca agatgacgac gacgggaatc agcgagtagc
    17761 ggcgtagagc gccatcgagc cttcttcgta gatgcgctcg acgctggcga actgggcggc
    17821 gcggaggact gcgagttgct cgtcgcgcga ccagctgagc ccggggagcg gcgagtcctc
    17881 ggtgcgataa tggtccgcgt agagcagcac gccgcccggc acgagcaacg ggaggatctg
    17941 gccgagcaag cgcggcatgt gtcgcctgtg gcgcagctcg tggacggcct gtagcgtgac
    18001 gaccgcgtcg aacgtgccga gcccctcgga ccacgtgtcg gagcgaaaat cgcgggtcac
    18061 ttgcgtcacc cgtggtgcga gctcgccgag gtgctcgcgc gcgagcgcat gcatcggttc
    18121 agcccagtcg agcgcgaggt agctcgcgat ctggcagcgg ctcacgatct cgcgcgcgag
    18181 gtggccgggg ccggagccga gctcgggcac gcgcggcggc gtggagcggg ccggcagagc
    18241 ggtacagaac gccgtgaaga agttcacgcg gtaggggcgc ttcttcggcg tctcctcggt
    18301 ccacgtacgt gcggtcgcgg cgtcgcggaa gtcgacgcgg agcggaacgt cttcgtcgtc
    18361 ggtcacccga tcctcgcgag cgcttcattc attgcttcga taagtacctc gagatcgcga
    18421 attgtcgcgt cgtgatcggc ggcgagcgga tcgccaacga caagcacgtg actcacggta
    18481 tcgcgcgtca tgaacggcgc cgctgtcggc tgcgcggcat tgtcgagcgt gaccgcgagt
    18541 cgcttggctg cgtctgtggg tgtctcgtcg gcgatgcgtc gctcgtcgcg tgcgagctgg
    18601 acgaccgacg gctcgctgag cggcaggatg aagctgcggg tcgcgctcgg cttgagccgc
    18661 tgtccgtggc cgcgcagtcc gatggcgcgc ttgtcttcga tcgcgacaat gagcgcggct
    18721 gaccatcgct tcgcgacgta gcccatcacg acgtcgagca gccattcgag gtcgtcgatg
    18781 tcggggaacg acgcgatcgt ttcgtcgagc gagtcgcgcg cggtttcggt ggtgagccgt
    18841 ttgatcctga ccggcagggc gcggtttttt ggcttcggcg cctcgggggc ttcgatctcg
    18901 accggaatgt cgagctcggg ttcctcgacc tcgatttcga agtcgaccgg cggcggctcg
    18961 tcgacatcga tcggcacgtc gacctcggtg tagacgtgat cgacgagccg ctcgaggtag
    19021 agcgcgggcg cgactgcgag catgatcggc tggccgatca cgcgggcgag gtcatcgtgg
    19081 agctgcgacg acggatcgcg cgcgcagacg agcagtgtgc cgtctcccat cctgccgatt
    19141 ggaatcgcga ccgcacgctt gccgatggcg gaggggagca gggcggccac gctgcgatcg
    19201 cggtgcacga ggtggcggcg tagcgcggcc ggcgcgtgcc attgctcgcc gagcgcctgc
    19261 gaggcctgat cgaagtcgag cagccgccgc accacgagca gcgagcacag ccgcatccgg
    19321 ttcgggcgtt ggtcggtgag cgccgtcgcc agggcgtccc agtcgagcaa accacgttcg
    19381 agcaggatct cgccgatgcg catccggacg acggagcgta acccgtctga aatgtgccca
//
LOCUS       DASUFX010000020         5850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_3722,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000020 DASUFX010000000
VERSION     DASUFX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFV99_00835"
     CDS             <1..340
                     /locus_tag="VFV99_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057864.1"
                     /translation="NERGSLVCDDEIDADGDGEAAFATVGQFDTGCSSPFDTSELSTL
                     QCDDGIDNDGDGFIDDRTVNGDPGCSSTTDDDEHGVAQCDDGLDNDTDGKIDAMDPQC
                     TDPTDASEAM"
     gene            complement(356..1639)
                     /locus_tag="VFV99_00840"
     CDS             complement(356..1639)
                     /locus_tag="VFV99_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5057865.1"
                     /translation="MPARDPIALLLAGQYPDPTTGELLAAESRAVVIETSLAGSEADL
                     VAGLGVGTHVTVISDRNTHAVLGARVERALSSRFAMQSLVLDAGPRADDATVERVLAA
                     LSPSTDLVVAVGSGTLNDLTKMVAFRRGVPQVIFATAPSMNGYTSLSASITTAGIKTS
                     FRTRTPLGVYFDLRVLAAAPARLIRAGLGDSACRSTAQADWLLSHLLLDRPYRETPFA
                     LLADDERDLFGDTRALLAGDLEQMRHLVRTLVLSGFGMTICDGSYPASQGEHLLSHYV
                     DMMRPSDTLHGEQIAVCTVAMATLQEQLLARDTPPLLRPSSLRHEDLVRHYGPVIGDA
                     CWREFEQKHFDRETTDALNMKLARDWDTMRERIRAVSVGATKLRSLLAAAGAPVEPVE
                     LGWSAVVMQHALEHAREIRNRYTFLDLVADSASAR"
     gene            complement(1626..2291)
                     /locus_tag="VFV99_00845"
     CDS             complement(1626..2291)
                     /locus_tag="VFV99_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057866.1"
                     /translation="MDFVFMLTRADRTISDGLAVLDDIRSLGLRHIGFKDVGIEPRAA
                     AELHRRIKEMGATSYLEVVSTTPEACLQSARVARELGVDRLLGGTQVEDVLAILAGSN
                     IQYLPFPGYPFGHPTQLRGSTEDIARDCKRYRSLGAGGVDLLAYRAVEADPIELVRAA
                     RQASDGFLLVAGGVATRDQVRALAAAGADAFTVGSAAFDGSFAPHVGSLRSQLKAVLD
                     ACA"
     gene            complement(2307..3674)
                     /locus_tag="VFV99_00850"
     CDS             complement(2307..3674)
                     /locus_tag="VFV99_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057867.1"
                     /translation="MMRAVLALSFALLACGSEKKTGDDDGSADASVEADAMPDAFVPT
                     CDPDADPYGRCVGDMLATCTAGVPGTIDCTGMGKVCAWQDDTVGYACVDLANQFLVSG
                     TVSYEDKPPMNDGSLGPIAPHPARGARVSVVLDQGSTVLASATVADDGTYRLRYTTTA
                     GDMVHIMASAQSPLPARPVTVQTRQKGVHGFGGTSFAAAAATTSDVLVTDASGKSEAF
                     NILDQLVGTMDKIRVVLGDATPVPLTAQWAVGSTDGTYYDQGAIHLLGQASDDDGYDD
                     TVILHESGHYVEDTQGRSDSPGGSHDGSPTDPRLAWSEGFSTYWGMVVRGAPHYMDSN
                     ANGGWGYDADTTVTAAAMPAGTMSQDVSEDMVTEDLWDLGDGKPNDDDGVDDGHAAVL
                     RVQVDYLKPGVFRNVGVTGVDLVDALDGWFIKVGLPPCAEARGIITTTRTFPYDYAGP
                     AGACP"
     gene            complement(3671..4075)
                     /locus_tag="VFV99_00855"
     CDS             complement(3671..4075)
                     /locus_tag="VFV99_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057868.1"
                     /translation="MKVLLVVLMLAGHAHQKPTAPVAIELTQRSLGGTDYEVTLAARP
                     TRDVDSLELRLDGRIDRVVRARANVIHKLTVRVHLVDASQDVVGGATVRIGNLRRNVA
                     DIVTLGTPKTRPLPRSQIVTMPDGTKVEEVRP"
     gene            4277..4978
                     /locus_tag="VFV99_00860"
     CDS             4277..4978
                     /locus_tag="VFV99_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057869.1"
                     /translation="MRFSRFSCAAFVLILVGCNGDGVDEALLGDTSFRLTQECYDSTC
                     TPECEDTYCDVCDDSNPPSDGGCWVTGIGFLIDSDGKDNFGGNGMPMKHSYLRGEWEH
                     QDHGTGDKLHGKVMYLSCRHVDEPGPGQPSGPDHDFNINQAYYGGPGRWYEPATGWQE
                     GYWFDVMAEDHGEPGKTDEYYFSVRKMDASGAVGPVFYSTGGVLGGGNFQIHPPNDGH
                     PFTEGTTPAWVSLQP"
     gene            complement(4935..>5850)
                     /locus_tag="VFV99_00865"
     CDS             complement(4935..>5850)
                     /locus_tag="VFV99_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5057870.1"
                     /translation="PTTLSLANIKKKGRTKLKQNPSFFDAKGIEATQHFATSKDGTKV
                     PYFQVAKKGLPMDGSTPTVMEGYGGFEVSLTPGYDSTAGAAWLERGGVLVVANIRGGG
                     EYGPAWHQAALRHDRQRAYDDFIAVAEDLIKRKVTSTPHLGINGGSNGGLLMGVMLVE
                     RPDLFGAIVCQVPLLDMKRYHKLLAGASWMEEYGDPENAEDWAALAKFSPYQNVKAGV
                     KYPRTLFTSSTRDDRVHPGHARKMVARMLEQGHDVLYYENTEGGHGGAADNEQHAFMT
                     ALAYTFFAKQLGLDLTAGAKPTQESSPP"
BASE COUNT          984 a         1917 c         1971 g          978 t
ORIGIN      
        1 gaacgagcgc gggtcgctcg tgtgcgatga cgagatcgac gccgacggcg acggcgaggc
       61 tgcgttcgcg acggtcggtc agttcgacac cgggtgctcg agcccgttcg acaccagcga
      121 gctgagcacg ctgcagtgtg atgatggcat cgacaacgac ggcgacgggt tcatcgacga
      181 tcgaacggtc aacggcgatc cgggttgctc gtcgacgacg gatgatgacg agcacggtgt
      241 tgctcaatgt gacgacggcc tcgacaacga caccgatggc aagatcgacg cgatggatcc
      301 gcagtgcacg gatccaaccg acgcaagcga agcgatgtaa gccgttgcgg ctgggctagc
      361 gcgctgacgc gctgtcggcg acgagatcga gaaacgtgta gcgattgcgg atctcgcggg
      421 cgtgctcgag cgcgtgctgc atcacgaccg cagaccagcc gagctcgacg ggctcgaccg
      481 gagcgccggc ggcggcgagc aagctgcgca acttcgtcgc accgacgctg accgcacgaa
      541 ttcgctcgcg catcgtgtcc cagtcacgcg cgagcttcat gttgagcgca tcggtcgttt
      601 cgcgatcgaa gtgcttctgc tcgaattcgc gccagcacgc gtccccgatc accggcccgt
      661 aatgacgaac gaggtcttcg tgccgcagcg agctcggccg caagagcggc ggcgtgtcgc
      721 gcgcgagcag ctgctcttgc aacgttgcca ttgcaaccgt gcacaccgcg atctgctcgc
      781 cgtgcagtgt gtcagacggc cgcatcatgt cgacgtagtg gctgagcagg tgctcgcctt
      841 ggctcgcagg gtagctcccg tcgcagatcg tcatgccgaa gcccgacagc acgagcgtgc
      901 gtacgaggtg gcgcatctgc tcgaggtcgc ccgcgagcag cgcgcgcgtg tcgccgaata
      961 gatcgcgctc gtcatcggcg agcagcgcga atggcgtctc gcgatacggg cgatcgagca
     1021 gcaggtgcga caggagccag tcggcttgcg cagtcgatcg gcacgcactg tcgccgagcc
     1081 cggcgcggat cagacgcgcg ggtgccgcgg cgagcacgcg cagatcgaaa tacacgccga
     1141 gcggcgtgcg cgtgcgaaac gacgtcttga taccggcggt cgtgatcgac gcagagagcg
     1201 atgtgtagcc gttcatcgac ggtgcggtcg cgaagatcac ctgcggcaca ccacgccgga
     1261 acgcgaccat cttcgtcagg tcgttgagcg tccccgatcc gacggcgacg acgaggtcgg
     1321 tcgatggcga gagcgctgcg agcacgcgct cgaccgtcgc gtcgtcggca cgcggccccg
     1381 cgtcgaggac aagtgattgc atcgcgaagc gcgacgagag cgcgcgttcg actcgcgcgc
     1441 cgagcacggc atgcgtgttg cggtccgaga tcacggtgac atgcgttccg acgccaagcc
     1501 cggcgacgag atccgcctcg ctgcccgcga gcgaggtctc gatcaccacc gcgcgcgact
     1561 cggccgcgag caactcgccc gtcgtcggat cgggatattg acccgcgagc aagagcgcga
     1621 tcgggtcacg cgcaggcatc gaggactgcc tttagctgcg agcgcagcga gccgacgtgc
     1681 ggcgcgaacg aaccgtcgaa cgccgccgag ccgaccgtga acgcgtccgc gcctgcggct
     1741 gccagcgccc tgacctgatc gcgggtcgcg acgccgccgg cgacgagcaa gaagccatca
     1801 ctcgcttgtc gcgcggcgcg cacgagctcg atgggatccg cttcgacggc gcggtacgcg
     1861 agcagatcga cgccgccggc accgagtgag cggtagcgct tgcagtctcg cgcgatgtct
     1921 tcggtggagc cacgcagctg tgtcgggtga ccgaacggat agcctgggaa cggcagatac
     1981 tggatgttgc tgccagcgag gatcgcgagc acgtcttcga cctgcgtgcc accgagaagg
     2041 cgatcgacgc cgagctcgcg ggcgacgcgc gccgattgca aacacgcttc cggcgtcgtg
     2101 ctcacgacct cgaggtagct cgtcgcgccc atctccttga tgcggcgatg gagctcggca
     2161 gccgcgcgcg gctcgatgcc gacatccttg aagccgatgt gccggaggcc gagcgaccgg
     2221 atgtcatcga gcaccgcgag tccgtcgctg atcgtgcgat cggcgcgggt cagcatgaac
     2281 acgaagtcca tagggcccag actgtactag ggacaggcgc cggcagggcc tgcgtagtcg
     2341 tacgggaacg ttcgcgtcgt cgtgatgatg ccgcgtgctt cggcgcaggg cggcaggccg
     2401 accttgatga accagccatc gagcgcatcg acgagatcga cgccggtgac gccgacgttg
     2461 cggaagacac ccggcttcaa gtagtcgacc tgcacgcgca gcaccgcggc gtgaccgtcg
     2521 tcgacgccat cgtcgtcgtt cggcttgccg tcgccgagat cccacagatc ctcggtgacc
     2581 atgtcctcgc tcacgtcctg gctcatcgtt cccgccggca tcgccgcggc ggtcaccgtc
     2641 gtgtcggcgt cgtaacccca gccgccgttc gcgttcgagt ccatgtaatg aggagcgccg
     2701 cgcacgacca tgccccagta cgtcgagaac ccttccgacc acgcgaggcg cggatcggtc
     2761 ggcgagccgt cgtgcgagcc accgggcgaa tcagagcggc cctgcgtgtc ttcgacgtag
     2821 tgaccgctct cgtggaggat cacggtgtcg tcgtagccat cgtcgtcgga cgcctggccg
     2881 agcagatgaa tcgcgccctg gtcgtagtac gtgccgtcgg tgctaccgac cgcccattgc
     2941 gcggtcagcg gcaccggtgt ggcgtcgccg agcacgacgc ggatcttgtc catcgtgcca
     3001 acgagctgat cgaggatgtt gaacgcctcg gatttgcccg acgcatcggt gacgagcacg
     3061 tcgctcgttg tcgccgccgc ggccgcgaag ctcgtgccgc cgaaaccgtg cacgcctttc
     3121 tgccgcgtct ggacggtgac aggacgcgcg ggcaatgggc tttgcgccga cgccatgatg
     3181 tgaaccatgt cgccggcggt cgtggtgtag cgcaggcgat acgtgccgtc gtcggcgacg
     3241 gtcgcgctcg cgagcacggt gctgccctgg tcgagcacga cgctgacacg cgcgccgcgc
     3301 gcaggatgcg gtgcgatcgg gccgagcgag ccgtcgttca tcggcggctt gtcctcgtac
     3361 gagaccgtgc ccgacacgag gaactgattc gcaagatcga cgcacgcgta cccgaccgtg
     3421 tcgtcctgcc acgcgcacac cttgcccatg ccggtgcagt cgattgttcc cgggacacct
     3481 gcagtgcacg ttgcgagcat gtcgccgacg caacgcccgt acggatcggc gtccggatcg
     3541 catgtcggca cgaaggcgtc gggcatcgca tcggcttcga ccgatgcatc ggcgctgccg
     3601 tcgtcgtcgc cggtcttctt ctcgctgccg cacgcgagga gcgcgaacga tagcgcgagc
     3661 acagcgcgca tcacggccgt acctcctcga ccttcgtgcc gtcgggcatc gtgacgatct
     3721 gcgagcgtgg gagcgggcgc gtcttcggcg taccgagcgt cacgatgtct gcgacgttcc
     3781 ggcgcaggtt gccgatgcgc acggtcgcgc caccgacgac gtcctggctg gcgtcgacga
     3841 gatgcacccg gaccgtgagc ttgtggatga cgttggcacg tgcacgaacc acccggtcga
     3901 tgcggccgtc gaggcgaagc tcgagcgaat cgacgtcacg cgtcggtcgc gccgcgagtg
     3961 tcacctcgta gtccgtgccg cctagcgagc gttgcgtgag ctcgatcgcc actggggcag
     4021 ttggtttctg gtgagcatgc cccgcgagca tcagcacaac gagcagcact ttcatcagaa
     4081 cgagtatccg tcagtttccg acgaggagcg agcgatccgg ctggctcacg acgccccatg
     4141 gtgaacggtc acctcgcact tagtcgccag tgcgctcatg ttcgtgtcgc aagcatccgt
     4201 cttcgtgtgc tcgaccgctg gttgattgct ggcccgccga gtgctttgcc gcgctctacg
     4261 gaaagtgagc ggctccatgc gtttctctcg attcagctgt gcagcgttcg tactgatact
     4321 cgtcggatgc aacggtgatg gggtcgacga ggccttgctc ggcgacacct cgtttcggtt
     4381 gacgcaggag tgctacgact cgacgtgcac gccggagtgc gaagacacgt actgcgatgt
     4441 ctgcgacgac tccaacccgc cgagcgatgg cggctgctgg gtcaccggca tcggctttct
     4501 catcgacagc gatggcaagg acaacttcgg cggcaacggc atgccgatga agcacagcta
     4561 cctgcgcggc gagtgggaac accaggacca tgggaccggc gacaagctgc acggcaaggt
     4621 gatgtacctg tcatgccgcc acgtcgacga gcctggcccc ggtcagccga gcggacccga
     4681 ccacgacttc aacatcaacc aggcctatta cggcggcccg ggtcgctggt acgagcccgc
     4741 caccggctgg caggaaggtt actggttcga cgtgatggcg gaagatcacg gcgagccggg
     4801 caagaccgac gagtattact tcagcgtcag aaagatggac gcgagcggcg cggtcggccc
     4861 ggtgttctac agcactggcg gcgtgctcgg cggtggcaac ttccagatcc acccaccgaa
     4921 cgacggccac ccgttcacgg aggggacgac tcctgcgtgg gtttcgctcc agccgtaagg
     4981 tcgagtccga gctgcttcgc aaagaacgtg tacgcgagcg cggtcatgaa cgcgtgctgc
     5041 tcgttgtcgg cggcaccacc gtgcccacct tccgtgttct cgtaatagag gacgtcgtga
     5101 ccctgctcga gcatgcgcgc gaccatcttg cgcgcgtgac ctgggtgcac gcggtcgtcg
     5161 cgcgtcgatg acgtgaacag cgtacgcgga tacttcacgc ctgccttcac gttctgatac
     5221 ggcgagaact tcgcgagcgc ggcccaatct tctgcgttct ccggatcgcc gtactcttcc
     5281 atccacgacg cgccggcgag cagcttgtgg tagcgcttca tgtcgaggag cgggacctgg
     5341 cacacgatcg cgccgaacag atcgggacgc tcgacgagca tcacgcccat gagcaggccg
     5401 ccgttcgagc cgccgttgat gccgaggtgc ggcgtcgagg tcaccttgcg cttgatcaga
     5461 tcttcagcga ccgcgatgaa gtcgtcgtac gcgcgctggc gatcgtgacg gagcgccgct
     5521 tgatgccacg ccggaccgta ctcgccgccg ccgcggatgt tcgcgacgac gagcacgccg
     5581 ccgcgctcga gccacgcggc tccggcggtc gagtcgtacc cgggcgtcag cgagacctcg
     5641 aacccgccgt agccctccat caccgtcggc gtcgagccgt ccatcggcag gcccttcttc
     5701 gcgacctgga agtacgggac cttcgtgccg tccttcgacg tcgcgaagtg ctgcgtcgcc
     5761 tcgatgccct tggcgtcgaa gaacgacggg ttctgcttga gcttggtgcg gcccttcttc
     5821 ttgatgttcg cgagcgacag cgtcgtcggc
//
LOCUS       DASUFX010000021         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1114892, whole genome shotgun sequence.
ACCESSION   DASUFX010000021 DASUFX010000000
VERSION     DASUFX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114892"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFV99_00870"
     CDS             <1..567
                     /locus_tag="VFV99_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5057871.1"
                     /translation="FSRATEFVPSARYTISYVLVEPVDAAAIPGIQSAVHDLGYLALT
                     EHQFEDRIANFYKYKTGLGMNIMLMTVISFIVGLSISAQTFYAFVLEHLERFGALKAI
                     GAKGRELVYMILFQVGVASFAGYGLGVGLCSLLIALAKLRLPSYASVVTYGNLGLAFV
                     MVLVIAGVSSYLGIRRVLRIEPFDIFRA"
     gene            564..1268
                     /locus_tag="VFV99_00875"
     CDS             564..1268
                     /locus_tag="VFV99_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5057872.1"
                     /translation="MTMAELAIKATGLTKWFGEGDARTVAVRDVSFEAYFGEMLFLVG
                     PSGSGKTTLLSMISGVLRPNEGSVQVEGKDIWSLSDNQLAEFRLHTVGFVFQDYHLFP
                     RLTTVENVAIPMILRKQQWNEAIARARDELEIVGLANRAEINPGKLSGGEQQRVAIAR
                     ALVARPDILILDEPTASLDGETGKKIMGFVKTEVLNDKRCILTVTHDSRIFEFADRVM
                     SMADGQIVGTEVPAHA"
     gene            1261..2391
                     /locus_tag="VFV99_00880"
     CDS             1261..2391
                     /locus_tag="VFV99_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5057873.1"
                     /translation="MRNKLVFSLVGLGLAGGFVSAYVYGQKSVPQPPVFTPPSNPFVS
                     GVYAEGIVESYQEHGQNTNIFPEVTGVVVDVRVNEGQHVAKGDVLLALDDSVQRATTE
                     QLAAQTLAAQAVLDELRAEPRRETLAVAKSQADLAAANEKTAKDALDKAQRAHDLDPR
                     SVSKQELDNAINAFESARASHDVAKRQYELTKAGAWIYDIRNQERQVEALRKQTASAQ
                     ALLDKYTLRAPRGGRVMTIGSAVGSYASPQGVYNPYTQGFDPIITMADETTLAVRCYV
                     DEVLVPRLPDATKVAAHLFVRGTNISVPLQFVRVQPFVTPKIELSNARTEKVDLRVLP
                     VLFRFAPPAETTLYPGQLVDVYIGAGVPPTTTLARPGQGRQR"
     gene            complement(2401..>3126)
                     /locus_tag="VFV99_00885"
     CDS             complement(2401..>3126)
                     /locus_tag="VFV99_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5057874.1"
                     /translation="MISKYLRVAVVYAEDVRFFQHGGVDWDAVQAAAGDHFRIGGSTI
                     TQQLAKNLYLSPSRSLIRKAREMLIAFALEDHLTKQRILELYLNVAEWGDGVFGAEAA
                     AQTWFHHSAQQLSPSEAAQLAIALPNPFTRSPTVHTEELIRKAVRIIRLFRMQGLINA
                     AQERAALDEVGAQSARVRPDTSLPPEPQPPAPSPPAGTPQTPQPPAPAQASPPPPTSP
                     AASPSAESPPASEPASEPASATP"
BASE COUNT          528 a          986 c         1075 g          537 t
ORIGIN      
        1 ttcagccgcg cgaccgagtt cgtgccgtcg gctcgctaca cgatctcgta cgtgctcgtc
       61 gagccggtcg acgccgcggc gattcccggg atccagagcg cggtgcacga cctcggctac
      121 ctcgcgctga ccgaacacca gttcgaggat cggatcgcga acttctacaa gtacaagacc
      181 ggcctcggta tgaacatcat gctgatgacg gtgatcagtt tcatcgtcgg gctttcgatc
      241 tcggcacaga cgttctacgc gttcgtgctc gagcatctcg agcgcttcgg tgcactgaag
      301 gcaatcggcg cgaaggggcg cgagctcgtc tacatgatct tgttccaggt cggcgtcgcg
      361 tcgttcgccg gctacggcct cggcgtcggg ctgtgctcgc tgctcatcgc gctcgcgaag
      421 ctgcggctgc cgtcgtatgc gtcggtcgtc acgtacggca acctcgggct cgcgttcgtg
      481 atggtgctcg tgatcgcggg cgtgtcgagc tacctcggga tccggcgagt tctgcggatc
      541 gaaccgttcg acatcttcag agcatgacga tggcagagct cgcgatcaaa gcgaccgggc
      601 tgacgaaatg gttcggcgaa ggcgacgcga ggacggtcgc cgtccgcgac gtgagcttcg
      661 aggcgtactt cggcgagatg ctgtttctcg tcggtccgtc aggtagcggc aagacgacgc
      721 tgctcagcat gatctcgggc gtcctgcgcc cgaacgaagg cagcgtccag gtcgagggta
      781 aagacatctg gagtctcagc gacaaccagc ttgccgagtt ccgcctgcac acggtcggct
      841 tcgtgtttca ggactatcat ctgttcccgc ggctcacgac ggtcgagaac gtcgcgatcc
      901 cgatgatcct gcggaagcag cagtggaacg aggcgatcgc ccgcgcccgc gacgagctcg
      961 aaatcgtcgg cctcgcgaat cgcgccgaaa tcaatccagg caagctgagc ggcggtgagc
     1021 agcagcgcgt cgcgatcgcg agggcgctcg tcgcgcggcc cgatatcctg atcctcgacg
     1081 agccgacggc gtcgctcgac ggcgagaccg gaaagaagat catggggttc gtcaaaacgg
     1141 aggtgctcaa cgacaagcgc tgcatcctca ccgtgacgca cgacagtcgc atcttcgagt
     1201 tcgctgaccg cgtgatgagc atggccgacg gtcagatcgt ggggaccgag gtgccggcgc
     1261 atgcgtaaca agctcgtgtt ctcgctggtc ggcctcgggc tcgcgggcgg attcgtcagt
     1321 gcgtacgtct acggccagaa gtccgtgccg cagccgccgg tgttcacccc gccgtcgaat
     1381 ccattcgtca gcggcgtcta cgccgagggc atcgtcgagt cgtatcagga gcacggccag
     1441 aacacgaaca tctttcccga ggtcaccggt gtcgtggtcg acgtgcgcgt caatgagggc
     1501 cagcacgtcg cgaaggggga cgtgctactc gcgctcgacg actcggtgca gcgggcgacg
     1561 accgagcaac tcgccgcaca gacgctcgcg gcccaggccg tgctcgacga gctgcgcgca
     1621 gagccgcgcc gggagacgct tgccgtcgcg aagtcgcaag cggatctcgc agccgcgaac
     1681 gagaagacgg cgaaggatgc tctcgacaaa gcgcagcgcg cgcacgatct cgatccgcgg
     1741 tcggtgagca agcaggagct agacaacgcg atcaacgcgt tcgagtccgc gcgcgcgagt
     1801 cacgacgttg ccaagcgcca gtacgagctg acgaaggccg gcgcgtggat ctacgacatt
     1861 cgcaaccagg agcgtcaggt cgaggcgctg cgcaaacaga ccgcgtcggc gcaggcgcta
     1921 ctcgacaagt acacgctgcg cgctcctcgc ggcggtcggg tcatgacgat cggttcggcg
     1981 gtcggcagct acgcgtcacc gcaaggcgtc tacaacccgt acacccaggg attcgacccg
     2041 atcatcacga tggcggacga aacgacgctt gcagtgcgct gctacgtcga cgaagttctg
     2101 gttccgcgcc tgcccgatgc cacgaaggtg gctgcgcacc tgttcgttcg cggcacgaac
     2161 atcagcgtgc cgctccagtt cgtgcgtgtg cagccgttcg tgacaccgaa gatcgagctc
     2221 tcgaacgcgc gcaccgagaa ggtcgatctg cgcgtattgc cggtgctctt ccgctttgcg
     2281 ccgccggcag aaaccacgct gtatccgggc cagctcgtcg acgtctacat cggtgcgggc
     2341 gtgcccccga cgacaacact cgcgcggccc ggccagggga ggcagcgatg atgcgcgaga
     2401 tcacggggtc gcggatgcgg gctcggatgc gggctcggat gcgggcggcg attcggcgga
     2461 cggcgacgcg gccggggagg ttggcggtgg cggcgacgct tgcgccggcg ctggtggctg
     2521 tggcgtttgc ggcgtccccg ctggtggtga tggcgctggc ggttgcggtt ccggtggcag
     2581 cgaagtatcc gggcgtacgc gcgcggattg cgcgcctacc tcgtcgagcg ctgcgcgctc
     2641 ttgcgccgca ttgatcaggc cctgcatgcg gaacagccgg atgatgcgca cggctttcct
     2701 gatcagctct tcggtgtgca cggtcggcga gcgcgtgaac ggattcggca gtgcgatcgc
     2761 gagctgcgct gcttcgctcg gcgagagctg ctgcgcggag tggtggaacc aggtctgcgc
     2821 ggcggcctcg gctccgaaca cgccgtcgcc ccattcggcg acgttcaagt agagctcgag
     2881 aatgcgctgc ttcgtcaggt gatcctcgag cgcgaacgcg atcagcatct cgcgcgcctt
     2941 gcggatcagg ctgcgcgacg gcgagagata gaggttcttc gcgagctgct gcgtgatcgt
     3001 gctgccgccg atccggaagt ggtcgccggc ggcggcctgc accgcgtccc agtcgacgcc
     3061 gccgtgctgg aagaaccgga cgtcctcggc gtagacgacc gccacgcgga ggtacttcga
     3121 gatcat
//
LOCUS       DASUFX010000022        18625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_420702,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000022 DASUFX010000000
VERSION     DASUFX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18625
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420702"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..872
                     /locus_tag="VFV99_00890"
     CDS             168..872
                     /locus_tag="VFV99_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057875.1"
                     /translation="MRVGEVLLSKNGDTGIQFIELEDPFGESFPSSYTLQVFDAAGTQ
                     IGSSIALAVASGTTRMLIATAAAKTEFGTSANGPTLTVALSENGQACFARTGNVRIHC
                     VAWGCINTFIVATAQSRAPSPPDGQSAQIQSNGFYQLATPTPDTANTVGTTASNCPSE
                     PDAPPPVDGPVTGGDGGVPPDAVDNGAGDAGNNGGNNNGDDGGGCCQVDGSRSALGAC
                     LLGLGVVLALRRSRRR"
     gene            1028..1597
                     /locus_tag="VFV99_00895"
     CDS             1028..1597
                     /locus_tag="VFV99_00895"
                     /inference="COORDINATES: protein motif:HMM:NF021540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEX5057876.1"
                     /translation="MLRTALLLSLLAACDVGSVLNEGGAQPDASGGGRMDSSGPVGTA
                     RLNVTLTTSQTATPVYQPANVLAVWVEDANGKIMKTIGRYADLRAIALVAWGQKAGTN
                     DVDALSGATRTSHTDPVTLTAWDVTNRQGQVIPDGTYTVRMEVSDTNAITTTANNQGT
                     FTFVKGPNPQMQTGLSNGGFTNVSISYTP"
     gene            complement(1598..2317)
                     /locus_tag="VFV99_00900"
     CDS             complement(1598..2317)
                     /locus_tag="VFV99_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5057877.1"
                     /translation="MFSKERSLQRTIDKAINKLAQQPDRWSALEKLREDSSEEALFGM
                     CKRFGITSMKGVEDEQEKNWVVDVLVEKGGASLAPLTRYMKSAEQLSFPLRAFERIAD
                     HDRIIKVVDEIFATEPPGYARFPDRRIDLIKWFCEWKGATEDEVLSRITPYLLDFDEN
                     VRFTAIDGLSSRDPKKIVGPLVDALIRPEEESGRIRRTIVEVLERTKAPLGPRAKEVA
                     TMLSGPLGDDFKIDGEIVKKR"
     gene            2420..3496
                     /locus_tag="VFV99_00905"
     CDS             2420..3496
                     /locus_tag="VFV99_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5057878.1"
                     /translation="MRFLVLACVVACSAPASKPREKTTNPDQCFYGCKPGESSGQTAQ
                     AGGTPTSASAQNPAAGAGGAGGGKLTPAGERAVLLRQAADLLDKAGQALADGNKNLAE
                     NLFSTAELLVGADALASLADQFRAGAPPRVTTPTIKVEKTTAQPRAVGSSEEEDEQDK
                     APPPPQVGSLTGTVQIDGKPLTGAYGLVTLEPASGKWKPRAPKRHVMAQRNREFNPRV
                     MAIAVGSTVAFPNFDNIFHNVFSTSPLGAFDLGIYKTGEAREYTFQKEGIIRIGCNLH
                     ANMTGYLVVVSAPAYVVTDDSGHFKFGRLAPGKYKLKAWSERSKTPITQDVTIKVGAN
                     TVNVGVAGDAPAGPAPDKFGGKRG"
     gene            3507..5372
                     /locus_tag="VFV99_00910"
     CDS             3507..5372
                     /locus_tag="VFV99_00910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057879.1"
                     /translation="MAIGALLVIAGGAAAGYGVTRKATGGLSPQASEAMGSSIAQLDG
                     DVKAARASVKERAATFASLLPVQAAVSTDASTAKDMLVRGELQFSPQAGEVIELGQVT
                     GDKAETLLIQPEGAMHSPHASKAGSYADLMGDKVVITEVAQVTPKEARDGLTGYVMVV
                     RPLDLKPAIDRLVGAGVAGTIEANGEKRTIGSPAPNAAMQTQAIPSLPEMKVIVAVPP
                     KTGGMNMPLAAGGGGLAALGLILLVVGLVAKPAGTAAAAAAAAGTQAPSLGTAATQMS
                     QPGMYATGTPSPVAPSGPIAATDLGPGAMIGRWEIIKRLGSGGMADVYLARAKGEAGF
                     EKTVAVKVMHPHLARMSRAVDHFLDEARLAARIAHPNVVAIQDLGKIGNDYVIVMEYV
                     DGVDLERLLTSARTAQRLVPIDVALGIMCRICDGLNAAHKCLAADGTPLNIVHRDVKS
                     ANVLVSKQGGVKVVDFGIAKAAQQGHNTIAGETKGTPSMMAPEQRVGEAVDVRADVYS
                     VAAVAYEILTGHAVNLDLAALAHLGVENWPHLPPPSSLRAQLPAELDGVLLKAMSFER
                     DKRPADCAALEATFEQIMKQYSLTASDKDIARWVQSEVALLSSSQPDAYSQTVAP"
     gene            5372..5995
                     /locus_tag="VFV99_00915"
     CDS             5372..5995
                     /locus_tag="VFV99_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX5057880.1"
                     /translation="MLLFKKAFFAGLTSGAITLTFRRWQKPHVRVGGRYRCHPIGVLE
                     VDRVDRVRAAAISADDAQRAGFATLPELRAYLEELGPLADDDELWRVELHYGGDGDRV
                     EIALADQLTEDDVQAIAKKLEKLDGRKPWTDKTLAIIGKHPRVAASKLAAKLGRETQP
                     FKIDVRKLKKLGLTQSFEVGYEISPRGKAYLTARAKSAPRRAARRAR"
     gene            6037..6417
                     /locus_tag="VFV99_00920"
     CDS             6037..6417
                     /locus_tag="VFV99_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057881.1"
                     /translation="MRWLVLAALVAACAADPTEDTTFEYRVGFDRVDGSVVVNGKPFT
                     ERLVFNADSFEVAQRDIVLDVSVTTAVGTLAQQLRPGYCDLAANGTPRLESLGYEVIE
                     STPPLLQVSWYLCEGSRGGAGGSP"
     gene            6439..7575
                     /locus_tag="VFV99_00925"
     CDS             6439..7575
                     /locus_tag="VFV99_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEX5057882.1"
                     /translation="MRVRLVWLGIVAGCWSSQAPVQSPPPSPPPPPLVVAAPPPMPVD
                     AGAPVVAAAPDAAAPVQLAAPPPATCGTDEVWEALCGGKGTGTGTCGPTSDTLASWGY
                     QRLVISVITKGKADPLLKRFHYSDAETQPYAASIGPEGGGLPGPFCCYTSCTPLVVAA
                     RAATPKAPAGHHVEDACISPPASTRVPAKQNASCPAAVEIAGVLAPYTGAHKPARGIW
                     WYRANGGKACCYSVVVQDPPPDFDRNCPNCKCAAAGTPIATPDGEVAIESLAPGDLVL
                     SMDRGALRAVPLARVHRQRVHDHVLVVATLANGRTVAMSPQHPTADGHHFADLAAGSL
                     LGGVRVVSVERVPYSGDYTYDLLPASDSATYVAAGALVGSTLKP"
     gene            complement(7564..8412)
                     /locus_tag="VFV99_00930"
     CDS             complement(7564..8412)
                     /locus_tag="VFV99_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057883.1"
                     /translation="MRGLRGLTTALFVLCAAAQAFAAPTPAPAPAPPVPPPAPPMPQP
                     DQPAAPAAEPDFAKATELYNAASQAMNESRFDDASRDFLAAYEITKDAVLFFKIGTAY
                     EKAGKCSAALGYYERYLAEAKPADNFATLTRERIDACKATLPQKSAETAPAAATPEPS
                     ATDIKPSKNKDRAWLFVGGSLAFATAGAVLAYSTASSEQDVKDLYVSNNGKPPEFDAE
                     TKERYDDLIAEGKRYQVLAWTSFGIAAGCAIGATIYFLRARNEDITVAPVVTPKETGV
                     SATLRF"
     gene            8473..10506
                     /gene="fusA"
                     /locus_tag="VFV99_00935"
     CDS             8473..10506
                     /gene="fusA"
                     /locus_tag="VFV99_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5057884.1"
                     /translation="MAKIDLVRNLGVVAHIDAGKTTVSERFLFHSGKIHKVGEVHDGE
                     TEMDWMEQERERGITITAAATTFEWKKHEIHLIDTPGHVDFTIEVERSLRVLDGAVVV
                     FCGVAGVQPQSETVWHQADKFKVPRLAFVNKLDRVGASFQGVVADIRERLGANAVPVQ
                     LPIGAEDQFSGCIDLVTMKAIMFSGDVTETPDIADIPSDLAAAAADARDQMIQSLADL
                     DDGIAAKYLEGQDIPEAELKAVIRKATIAVKMIPVLAGTALRNKGIHPLLDAVIDYLP
                     SPADVPPVTGVDPRNTSEKLTRAPKNNEPFAALAFKIAMDEGRKVVFMRIFSGMIEAG
                     AEILNVRTNKKEKVARLFRIHAQKRERLDKGIAGEIVAVAGLKDATTGDTICDPKAPI
                     LLERIDTYEPVISQAIEAETQAAKERLDFALQKMADEDPTFRVKEDTDTGQTIISGMG
                     ELHLEIIVDRMKREYGVLARAGKPQVVYRETVLASGDGTAEFERDMKEQAIYGQASCK
                     VVGRPRGSGMEFKKALPAGTLLSPVIIEAGMQGLRDAASSGPDGYPLEDVEVTLTALA
                     TKEGSNAEIGCRAAAAEAFRRAVAGAHPSRLEPIMAVEVTVDDEFLGAVIGDLNQRRG
                     HVQDVGSRGVKRVVQAHVALRNMFGYSTRLRSLTEGRATFSMQFHAYDTLQSA"
     gene            complement(10503..11816)
                     /locus_tag="VFV99_00940"
     CDS             complement(10503..11816)
                     /locus_tag="VFV99_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057885.1"
                     /translation="MRWGALLVAYVLAGCNSGTGVDIDIYAPDGVKVDRVELWIAYDQ
                     CGDCPNGVAWNDKERTSGGIYFLRDEALIRAEPRPDRFVIHLDAKPGFEDPAWITFVG
                     YDGDKVTAIKALQPEDNTFIPTSEVVIWKMDLHPADVAINDVDTPPPTDHNWRGLVWN
                     RAPTSALAEPTGCLVYQKWDGAAWRTDYLVPKTDPDCDGIPPEKECSDFWYQYKPNGS
                     CVSSMTPMFDGTCVIGRSPCADGVTSDTTCGLDTARSPTCLPDAFCAYCDDTIPADTC
                     IEHAVEQGYANAVLPHYDCGFDATPEGTPCLDQHATLQLPYVLSACGTIQMHTLDKPF
                     TEPQSSLTFGVAPDAVTFMAKLRTSTSEPCVVDVYWTGGITDTFKDGITFLLEVPYDN
                     GTRAMYPVNVQPTGNPISCPNQPLPLPCLVDGPTNDNVITCAASG"
     gene            complement(11843..13138)
                     /locus_tag="VFV99_00945"
     CDS             complement(11843..13138)
                     /locus_tag="VFV99_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057886.1"
                     /translation="MRGLVALLVLVIASCARGGVEFVVEPKNDTVTKVALYVGLGDAY
                     TESIMPPQHSTPFPKSSAWVRDNYNELDVREVSAGKPVVFQFQGGDKLGVVIAIGYAG
                     DTPVSAAVAHAIEVPREFIARYDLVLEPIANNANTASPLFLDVWQSTAGSPEAGKTCV
                     ALFDKRTTSADAVVTDLDPDCDGWRTGDAKECQPNFFMSYARPQLSDTACLVTERVVS
                     TDGTTNDACVLGGPPCRDGVGRENGCTAPSAYCMPKSVCGRCTNMTGNQWDCARDITP
                     LAAQYPSHIHCKLYYDAAGGLCPNPIKALSQPPANIGGHFCKTSGEFPQITSIGLDWN
                     NSIQFTDAGATLKVDVKNLQPNCNFEIVVSGSPGLRHTFAGLVAAELDNGRGVAVPIV
                     FDVDPLNVGCDAQIACQATWSWDLSELVDQCVNTPVFPP"
     gene            13243..14118
                     /locus_tag="VFV99_00950"
     CDS             13243..14118
                     /locus_tag="VFV99_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX5057887.1"
                     /translation="MAIRARAARGLRVAPQVQEVVASPIVKWVGGKTKLLPDLLSRMP
                     ERYGRYYEPFAGGAALFFRLAPKRAVLADSNPDLIGLYTAVANDVNAVIRRLQGHRDA
                     HDEAHYYDMRTRWNDRDHEWSTPERAAAFIYLNKTCFNGLWRVNRSGAFTVPIGRYTD
                     PPICVPDALRAAHHVLSRAEIRRADYRTAVRDAKAGDFLYFDPPYDPVTPTANFTSYT
                     ADSFGPDEQRALADTARELVARGCQVMLSNSDTPFIRSIYKGFRVDRVKCARAINSNA
                     AKRGDVDELIITGGY"
     gene            complement(14166..14447)
                     /locus_tag="VFV99_00955"
     CDS             complement(14166..14447)
                     /locus_tag="VFV99_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057888.1"
                     /translation="MSNEGELTAEDPAGRVPVMARAGNEQTYLLVFKNVVKARQFVSS
                     SSLEGAEPRMVVKGNRDDIMRIARSAGVVGTLLDYDPSTQQYAEAQALA"
     gene            14588..15694
                     /gene="moaA"
                     /locus_tag="VFV99_00960"
     CDS             14588..15694
                     /gene="moaA"
                     /locus_tag="VFV99_00960"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5057889.1"
                     /translation="MTRTHLPVLQATALRPPSSGNVVPADPLVDGFARRIRYLRVSVT
                     DRCNYRCTYCMPETLEDRMNFQPRAALLTFEELEQIVAVFARLGVRKIRLTGGEPTVR
                     HGIVDLVRRMTAVPGIEQVVMTSNGHLLAELAEPLRAAGLSAINISVDTLDADRFRTI
                     TSRGELSRVLAGIDAAVAAGLRVKTNAVALRGINEHELVALCEDAWRRGATPRFIEHM
                     PMSDGALYEAAQGISAAAIRRTVEAALGSLVAADDRPPKMEPAGFTPAGGVDAGPARY
                     WALAADPTKQLGIISAMTEHFCDDCNRLRLTATGELHACLGHDDAVSLRDVLRGGGSD
                     ADLVHAIAGAVQGKRAGHVFEAKAPAKHMIGIGG"
     gene            15775..16308
                     /locus_tag="VFV99_00965"
     CDS             15775..16308
                     /locus_tag="VFV99_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5057890.1"
                     /translation="MKQGTLAKLAVTIAVVAGGAGFMVYSSTKHAQHYEMVDALLAKG
                     FDQFKDKEIKVHGYVEAGTIAKATVNQETRHMFVLQKNGKKIRVFVSGPTPDTFKDAS
                     EVVATGRLVPATNMQAVADAICAKPDSKLPGECKDGAKVPADAEQTMVVEATELMAKC
                     PSKYKGADSNRIDTTYK"
     gene            16321..>18625
                     /locus_tag="VFV99_00970"
     CDS             16321..>18625
                     /locus_tag="VFV99_00970"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5057891.1"
                     /translation="MTDHSVPFSPIALVGTIALLTAYAVAAWCVAAGIAGNARKSRRL
                     VNSAVYGLYGFAALVGLASALIIYAFVTHDFSIKYVAATSDTTMSTWYKVTAFWGGLD
                     GSLLFWVLVLALFSTIAIAVNAKRHRDMIGFVVGTIMVVQVFFLSLLIFTKNPFSTYL
                     TTPPADGRGLNPLLQNYWMVIHPPSLYVGFVAATIPFSFGIGALASGRLDDMWLGSVR
                     VWVLICFGFLSLGLILGGRWAYEELGWGGYWAWDPVENAGFMPWFCATAFLHSAIIQE
                     QRGMMKTWNLVLVVVTFFLTIFGTFMTRSGAVQSVHAFGEDNELAINFIVFMALILIV
                     SIGLIVYRSSKLSTKTEFDSFYSREFAFLLNNWILLACAFFVLFATMFPTISEAIDGS
                     RVSVGPEFFNKWMTPFGLSLLFLAGAAPLLAWRKTTRERLWNQFMFPTGLMAATIVGL
                     LIAFPQTKETSSIFADKLEIPVSLINFGLIAFCVGSIGQEFWRGMLVRRKQTGSDPMT
                     SLIGLVLSKRRKYGGYIVHLGVAVMFFGFAGKAYDSMIDRTVDKPGAYNASNGTGDSS
                     FTFTVPKTDKAGNTIQDPVFVGPFSKSRYTFTYENLFHTSDDHKDAVTAQVGIYDYGR
                     RIGTVYPAKWDYHKGDGQMTTEVAIKVRVLEDVYLVLTGFDLESKQANFRVYLNPLIL
                     WVWVGFLILAFGTLVCLIPQRVVDLLQWKPKTRLGRAADVSIVASVCFAVVMGIASQA
                     HAQGEEQQSSGGEHVPAGMGMGDAGAGY"
BASE COUNT         3223 a         6135 c         6105 g         3162 t
ORIGIN      
        1 tgacgatcgg ccccgacccg atgatgagga tcgactcgag atccttccga cgtggcatcg
       61 ggacgaccgt ttaccacaca atgagaagca tgcgtcacct agtgcggata gttgtcttgg
      121 gcgtcgcgat ctgttcgtca acgcaggcat tcgctctcga ccacaacatg cgcgtcgggg
      181 aagttctgct gtcgaagaac ggcgataccg ggatccaatt catcgagctg gaggatccgt
      241 tcggcgagtc gtttccgagc tcgtatacgc tgcaggtgtt cgacgcggca ggtacccaga
      301 tcggctcgtc aatcgcgctc gcggtagcgt ccggtacgac gcgaatgctc atcgccaccg
      361 ctgctgcgaa gaccgagttc gggacgagcg caaacggacc cacgttgacc gtcgcgttgt
      421 ccgagaacgg gcaggcgtgc ttcgccagaa caggcaacgt gaggatccat tgcgtagcgt
      481 ggggctgcat caacacgttc attgtcgcca cggcgcaatc acgcgctccg tcgccgccgg
      541 atggccaatc ggcgcagatc cagagcaacg gcttctacca gctcgcgacg ccgacgcctg
      601 atacggcgaa cacggtaggg acgacggcgt ccaactgccc gtccgagccc gacgcgccac
      661 ctcccgtcga cgggccggtg accggtggcg atggcggcgt cccgcctgac gccgtcgata
      721 acggcgccgg cgatgccggc aacaatggcg gcaacaacaa cggcgacgat ggcggcgggt
      781 gctgccaggt cgatggctcg cgcagcgcgc tcggtgcctg cctgctcggg ctcggcgtgg
      841 tgctcgcgct gcgccgatct cgtcgccggt aagtccagcg atcgcgcgca cgtagtgcat
      901 tttgcggaca catgatttgc tgggggggac cggtggcagc tgcggagtga agccccggcc
      961 cctgggggcc gaatcgcgca gttcacacgg gttacaggtt gccccgacaa ttgcagaagg
     1021 agacggaatg cttcggaccg cgctgttgct ctcgctgctc gccgcgtgtg acgtcggctc
     1081 ggtcctgaac gagggtggcg cgcagcccga cgcgagcggc ggtggccgga tggacagcag
     1141 cggcccggtc ggcacggctc gactgaatgt cacgctaacc accagccaga cggcgacccc
     1201 ggtgtaccag ccggccaacg tgctcgcggt ctgggtcgag gacgcgaacg gcaagatcat
     1261 gaagacgatc ggccgctacg ccgacctccg agcaatcgcg ctcgtcgcgt ggggccagaa
     1321 ggccggcacg aacgatgtcg acgcgctgtc gggcgcgacg cgcacgagcc acacggatcc
     1381 ggtcacgttg acggcgtggg acgtgacgaa ccgccagggg caggtgatcc ccgatggcac
     1441 gtacaccgtg cgcatggagg tctccgacac caacgcgatc acgacgaccg cgaacaatca
     1501 gggcacgttc acgttcgtca aaggcccgaa cccgcagatg cagacgggcc tctcgaacgg
     1561 cggcttcacg aacgtctcga tcagctacac gccgtgatca gcgcttcttg acgatctcgc
     1621 cgtcgatctt gaaatcgtcg ccgagcggtc ccgacagcat cgtcgcgact tctttcgcgc
     1681 gcgggccgag tggcgccttc gtgcgctcga gcacctcgac gatcgtgcgg cggatgcggc
     1741 cggattcttc ctcggggcga atcagcgcat cgacgagcgg gccgacgatc ttcttcggat
     1801 cacgcgacga caggccgtcg atcgcggtga agcgcacgtt ctcgtcgaag tcgagtagat
     1861 agggcgtgat gcgcgacagg acctcgtcct cggtcgcgcc cttccactcg cagaaccact
     1921 tgatgagatc gatgcggcga tcgggaaagc gcgcgtagcc cggtggctcg gtcgcgaaga
     1981 tctcgtccac taccttgatg atgcgatcgt gatcggcgat gcgctcgaac gcgcgcagtg
     2041 ggaacgacag ctgctcggcg ctcttcatgt agcgcgtcag tggcgcgagg gatgcgccgc
     2101 ctttttcgac cagcacatcg acgacccagt ttttctcttg ctcgtcttcg acacccttca
     2161 tgctggtgat gccgaagcgc ttgcacatgc cgaacagcgc ttcctcgcta ctgtcctcgc
     2221 ggagcttttc tagcgctgac catcgatccg gttgctgggc gagcttgttg atcgccttgt
     2281 cgattgtccg ttgcagtgag cgctcttttg aaaacaaccc gaagaccata agaaaaaggc
     2341 tccaaatttc gctgggaagc taccaccgaa tccgccaggt tccgatctgg tcgggccgga
     2401 gtcccgtaca atatggccga tgcgatttct ggtccttgca tgtgtcgtcg cttgctccgc
     2461 tcctgcgagc aagcctcgcg agaagacgac aaatcctgac cagtgcttct acggctgtaa
     2521 gccgggtgaa tcttccggtc aaactgccca agcaggtggg acacccacgt ctgcgtctgc
     2581 gcaaaacccg gcagcgggcg ccggaggcgc ggggggcggc aagctcaccc cggctggcga
     2641 gcgtgcggtg ctgctacgcc aggccgccga cctgctcgac aaggccggtc aggcgctcgc
     2701 cgatggcaac aagaacctcg cggagaatct gttctcgacc gccgagctgc tcgtcggtgc
     2761 cgacgcgctc gcatcgctcg ccgaccagtt tcgcgccggc gcgcctccgc gggtgaccac
     2821 gccgacgatc aaggtcgaga agaccaccgc ccaaccgcgc gcggtcggca gctccgaaga
     2881 agaagacgaa caagacaagg caccgccacc gccgcaggtc ggcagcctca ccggcaccgt
     2941 acagatcgat ggcaagccac tgaccggcgc gtacggcctc gtcacacttg agccggcgag
     3001 cggcaagtgg aagccacgcg cgccgaagcg tcacgtgatg gcgcagcgta accgcgagtt
     3061 caacccgcgc gtcatggcga tcgcggtggg atccaccgtg gcgttcccga acttcgacaa
     3121 tatttttcac aacgtgttct cgacgtcgcc gctcggcgcg ttcgatctcg gcatctacaa
     3181 gaccggcgaa gcgcgcgagt acacgtttca aaaagaaggc atcatccgca tcggctgcaa
     3241 cttgcacgcg aacatgaccg gctatctcgt cgtcgtctcc gcgcccgcgt atgtcgtcac
     3301 cgacgacagc ggtcacttca agttcggccg gctcgcgccg ggcaagtaca agctcaaggc
     3361 gtggagcgag cgcagcaaga cgccgatcac gcaggacgtg acgatcaagg tcggtgcgaa
     3421 cacggtcaac gtcggcgtcg ccggcgacgc accagcgggc ccggcgcccg acaagttcgg
     3481 aggtaagcgt ggctaggacg cccctcgtgg cgatcggcgc actgctggtg attgcgggcg
     3541 gagcggcggc gggatacggc gtgacgcgca aggcgacggg tggtctgtcg ccgcaggcga
     3601 gcgaggcgat gggcagctcg atcgcgcagc tcgatggtga cgtcaaagcc gcgcgcgcga
     3661 gcgtgaagga acgggcagcg acgtttgcga gcctgttgcc ggtacaagcc gcggtgtcga
     3721 ccgatgcgag caccgcgaag gacatgctcg tgcgcggtga gctgcagttc tcgccgcagg
     3781 ccggcgaagt gatcgagctc ggacaagtga ccggcgacaa ggccgagacg ctgctcatcc
     3841 agcccgaggg tgcgatgcat tcgccgcatg ccagcaaggc gggcagctac gccgacctga
     3901 tgggcgacaa ggtcgtgatc acggaggtcg cgcaggtcac gccgaaggag gcgcgcgacg
     3961 ggctcaccgg ttacgtgatg gtcgtgcgcc cactcgatct caagccggcg atcgatcgtc
     4021 tggtcggcgc gggtgtcgcc gggacgatcg aagcgaacgg cgagaagcgc acgatcggat
     4081 cgcccgcgcc gaacgcggcg atgcagacgc aggcgatacc gtcgctgccc gagatgaagg
     4141 tcatcgtcgc cgtgccaccg aagaccggcg gtatgaacat gccgctcgcc gcgggtggtg
     4201 gcggtctcgc cgcgctcggg ttgatcttgc tcgtcgtcgg cctcgtcgcg aagccggcgg
     4261 gtacagccgc agccgcagcc gcagccgcgg gcacgcaggc gccatcgctc ggaactgcgg
     4321 cgacgcagat gagtcagccc ggcatgtacg ctacgggtac gccgtcgccg gtcgctccgt
     4381 cgggaccgat cgcggcgacc gacctcgggc ctggcgcgat gatcggtcgc tgggaaatca
     4441 tcaagcggct cggctccggc ggtatggcag acgtctacct cgcgcgcgcc aagggcgaag
     4501 cgggcttcga gaagaccgtc gcggtcaaag tcatgcaccc gcacctcgcg cgcatgtcac
     4561 gcgccgtcga tcacttcctc gacgaagcgc ggctcgcagc gcgcatcgcg catcccaacg
     4621 ttgtcgcgat ccaggacctc ggcaagatcg gcaacgacta cgtcatcgta atggagtacg
     4681 tcgacggcgt cgatctcgag cgccttctga cgtcggcgcg caccgcgcag cgcctggtcc
     4741 cgatcgacgt cgcgctcggc atcatgtgcc gcatctgcga cggcctcaat gccgcgcaca
     4801 aatgtctcgc cgccgacggc acgccactca acatcgtgca ccgcgacgtc aagagcgcga
     4861 acgtgctcgt gtcgaagcag ggcggcgtga aggtcgtcga cttcggtatc gcaaaagccg
     4921 cgcagcaagg tcacaacacg atcgccggcg agacgaaggg tacgccgtcg atgatggcgc
     4981 ccgagcaacg cgtcggtgaa gcggtcgacg tgcgcgccga cgtctactcg gtcgcggcag
     5041 tcgcgtacga gatcctgacc ggccatgccg tcaatctcga tctcgctgcg ctcgcgcacc
     5101 tcggcgtcga gaactggccg cacctgccgc cgccgtcgtc actgcgcgcg cagctgccgg
     5161 ccgagctcga cggcgtgttg ctcaaggcga tgtcgttcga gcgcgacaag cgcccggccg
     5221 attgcgcggc gctcgaagcg acgttcgagc agatcatgaa gcagtacagc ctcaccgcga
     5281 gcgacaagga catcgcgcgc tgggtgcaat ccgaagtcgc gctgctgtcg tcgtcgcagc
     5341 ctgacgcgta ctcgcagacc gtagcgccgt agtgctgctg ttcaagaaag cattcttcgc
     5401 cggcctcacg agcggcgcga tcacgctgac gtttcgccgc tggcagaagc cgcacgtgcg
     5461 cgttggcggg cgctatcgct gtcatccgat cggcgtgctc gaggtcgatc gtgtagatcg
     5521 cgtgcgcgct gcggcgatct ccgccgacga tgcacagcgc gccgggttcg cgacgctgcc
     5581 cgagctccgc gcgtacctcg aagagctcgg tccgctcgcc gacgacgacg agctgtggcg
     5641 tgtcgagctg cactacggcg gcgatggcga tcgcgtcgag atcgcactcg cggatcagct
     5701 caccgaagac gacgtgcagg cgatcgcgaa gaagctggag aagctcgacg gccggaagcc
     5761 gtggaccgac aagacgctcg cgatcatcgg caagcacccg cgcgtcgccg cgtcgaagct
     5821 ggccgccaag ctcggccgcg aaacccaacc gttcaagatc gacgtgcgca agctcaagaa
     5881 gctcggcctt acccagagct tcgaggtcgg gtacgagata tcgccgcgcg gcaaggccta
     5941 cctcactgcc cgcgccaagt ctgcgccgcg gcgtgcggct cggcgggccc gctgaatcaa
     6001 acgcactgcg cgggtggcga gggttatgct cggggaatgc gctggctcgt actggcagct
     6061 ctcgtggccg catgcgcggc tgatccgacg gaagacacca cgttcgagta ccgcgtcggt
     6121 ttcgatcgcg tggacggcag cgtcgttgtc aacggcaagc ccttcacgga gcgtctcgtc
     6181 ttcaacgccg acagcttcga ggttgcgcag cgtgacatcg tgctcgatgt ttcggtcacg
     6241 acggcggtag gcacgcttgc gcagcagctg aggccgggct actgcgacct cgcggcgaac
     6301 ggtacgcctc gcctcgagag ccttggttac gaggtgatcg aatcgacacc gccgctcctc
     6361 caggtctcgt ggtacctgtg cgaaggcagt cgtggtggcg ccggtggctc gccgtagtac
     6421 tcgatctaca atgagcgcat gagggtgcgc cttgtgtggc tcgggatcgt tgccggttgt
     6481 tggtcgtcgc aggcgccggt gcaatcgccg ccgccatcac cgccgccacc gccgctcgtc
     6541 gtcgcggcgc caccgccaat gcctgtagac gcgggtgcgc cggtcgtcgc ggcagcaccc
     6601 gatgcggccg cacccgttca gctcgcggcg ccaccgccag cgacgtgcgg caccgatgaa
     6661 gtgtgggaag cgctgtgcgg cggcaaaggt accggcacgg gcacgtgcgg ccccacctcg
     6721 gacacactcg cgagctgggg gtatcagcgt ctcgtgatca gcgtgatcac gaagggcaaa
     6781 gccgatccgc tgctcaagcg cttccactac agcgacgccg agacgcaacc gtacgccgcg
     6841 agcatcggcc cggagggcgg cgggctaccg ggacccttct gttgctacac gagctgcacc
     6901 ccactcgtcg tcgctgcgcg cgcggcaacg ccgaaggcgc ccgcgggaca tcacgtcgaa
     6961 gacgcgtgca tctcgccgcc ggccagtacg cgtgtgcccg cgaagcagaa tgccagctgt
     7021 cccgccgccg tcgagatcgc cggcgtgctt gccccataca ccggcgcgca caagccagcg
     7081 cgcggcatct ggtggtaccg cgcgaacggc ggcaaggcct gctgctacag cgttgtcgtg
     7141 caggatccgc cgcctgactt cgatcgcaat tgtccgaact gcaagtgcgc cgccgccggt
     7201 acgccgatcg cgacgcccga cggcgaggtc gcgatcgaat cactggcgcc gggcgatctc
     7261 gtactgagca tggatcgcgg tgcgctgcgc gcagtgccgc tcgcccgcgt ccatcgccag
     7321 cgcgtgcacg atcacgtgct tgtcgtcgcg acgctcgcga acggccgcac ggtcgcgatg
     7381 tcaccgcagc atccgaccgc cgacggtcat cacttcgccg atctcgccgc cggctcgttg
     7441 ctcggcggcg tgcgcgtcgt ttctgtcgag cgcgtgccgt atagcggcga ctacacgtac
     7501 gatctgttgc cagcgtccga cagcgcgacc tacgtggccg ccggtgcgct cgtgggcagt
     7561 acgctaaaac cgtagcgtcg cggagacgcc ggtctccttc ggcgtcacga caggcgcaac
     7621 cgtgatgtct tcgttgcgcg ctctgaggaa gtagatcgtc gcgccgatcg cgcagccggc
     7681 tgcgatgccg aacgaggtcc acgcgagcac ctggtagcgc ttgccctcgg cgatcagatc
     7741 gtcgtagcgc tccttcgtct cggcatcgaa ctcgggcggc ttgccgttgt tgctgacgta
     7801 gaggtccttg acgtcctgct cggacgacgc agtcgagtac gcgagcacgg cgccggccgt
     7861 cgcgaacgcg agcgagccac cgacaaacag ccacgcgcga tccttgttct tcgatggctt
     7921 gatgtcggtc gccgacggct ctggcgtcgc cgcggccggg gcggtctcgg cagacttctg
     7981 cggcagcgtt gccttgcagg catcgatgcg ctcgcgggtg agcgtcgcga agttgtctgc
     8041 cggctttgcc tcggcgaggt agcgctcgta gtagccgagc gcggcgctgc acttgccggc
     8101 tttctcgtag gcggttccga tcttgaagaa cagaaccgcg tctttcgtga tctcgtaggc
     8161 cgcgaggaaa tcgcgcgacg catcgtcgaa ccggctctcg ttcatcgcct gagacgccgc
     8221 gttgtagagc tcggtcgctt tcgcgaagtc cggctcagcg gccggggcag ctggctgatc
     8281 gggctgcggc atcggcggag cgggcggcgg caccggcgga gcgggcgcgg gcgcaggcgt
     8341 cggtgccgca aatgcctgtg ccgccgcaca cagcacgaac agagcggtcg tcaggccacg
     8401 caagccacgc atgtccctga gcgtacagat ctcgccgcag atgcgctata ccgcctcccc
     8461 ggagtatccc gaatggcgaa gatcgatctc gtccgcaacc tcggtgtggt cgcccacatc
     8521 gatgccggca agaccacggt gtcggaacgc ttcctgttcc attcgggaaa gatccacaag
     8581 gtcggcgaag tccatgacgg cgagaccgag atggactgga tggagcagga gcgcgagcgc
     8641 ggcatcacga tcaccgcggc cgcgaccacg ttcgagtgga agaagcacga gattcacctg
     8701 atcgacacgc cgggccacgt cgacttcacg atcgaagtcg aacgctcgct gcgcgtgctc
     8761 gacggtgcgg ttgtcgtgtt ctgcggcgtc gccggcgtgc agccgcagtc ggagacggtc
     8821 tggcaccagg cggacaagtt caaggttccg cgcctcgcgt tcgtcaacaa gctcgatcgc
     8881 gtcggtgcgt cgttccaggg cgtggtcgcc gacattcgcg agcgcctcgg cgcgaacgcg
     8941 gtgccggtcc agctgccgat cggcgctgag gatcaattca gcgggtgcat cgatctcgtc
     9001 acgatgaagg cgatcatgtt ctcgggtgac gtcaccgaga cgcccgacat cgccgacatt
     9061 ccgagcgacc tcgccgctgc ggccgccgat gcgcgcgatc agatgatcca gtcgctcgcg
     9121 gatctcgatg acggcatcgc cgcgaagtac ctcgagggtc aggacatccc cgaggccgag
     9181 ctcaaagcgg tcattcgcaa ggcgacgatc gcggtgaaga tgatcccggt gctcgccggt
     9241 acggcgctgc gcaacaaagg catccatccg ctgctcgacg cggtgatcga ttacctgccg
     9301 tcgcccgccg acgtgccgcc cgtgaccggt gtcgatccgc gcaacacgag cgagaagctc
     9361 acccgcgcgc cgaagaacaa cgaaccgttc gccgcgctcg cattcaagat cgcgatggac
     9421 gaaggccgca aggtcgtgtt catgcgcatc ttcagcggca tgatcgaagc gggcgccgag
     9481 atcttgaacg tccgcacgaa caagaaggaa aaggtcgcgc gcctgttccg cattcacgca
     9541 cagaagcgcg agcgcctcga caagggcatc gctggcgaaa tcgtcgccgt ggcgggtctc
     9601 aaggatgcga cgaccggcga cacgatctgc gatccgaaag cgccgatcct gctcgagcgc
     9661 atcgacacct acgagcccgt gatctcgcaa gcgatcgaag ccgagaccca ggccgcgaag
     9721 gagcggctcg atttcgcgct gcagaagatg gccgacgagg atccgacgtt ccgggtcaag
     9781 gaagacaccg acaccggcca gacgatcatc tcaggcatgg gcgagctcca cctcgagatc
     9841 atcgtcgacc gcatgaagcg cgagtacggt gtgcttgcgc gcgctggcaa gccgcaggtc
     9901 gtctatcgcg agaccgtgct ggcgagcggc gatggcaccg ccgagttcga gcgcgacatg
     9961 aaggagcagg cgatctacgg ccaggcgtcg tgcaaggtcg tcggccgtcc gcgcggcagt
    10021 ggcatggagt tcaagaaggc gctgcccgcc gggacgctgc tatcgcccgt gatcatcgaa
    10081 gccggtatgc agggcctccg cgacgccgcc tcgagtggcc ccgacggtta tccgctcgag
    10141 gacgtcgagg tcacgctcac cgcgctggcg acgaaggaag gctcgaacgc cgagatcggt
    10201 tgtcgtgcag ctgccgccga ggcgttccgc cgtgcggtcg ccggcgcgca tccgtcgcgg
    10261 ctcgaaccga tcatggcggt cgaggtcacc gtcgacgacg agttcctcgg cgccgtgatc
    10321 ggcgatctca accagcgtcg cggtcatgtt caggacgtcg gctcgcgcgg cgtcaagcgc
    10381 gtggtgcaag cgcacgtcgc gctccgcaac atgttcggct actcgacgcg cctgcgcagc
    10441 ctcaccgagg gccgcgcgac gttctcgatg cagttccacg cctacgacac actgcagtcg
    10501 gcttagccgc tagctgcgca cgtgatcacg ttgtcgttcg tcggcccgtc gaccaagcag
    10561 ggcaggggca acggctgatt cgggcacgag atcgggttgc ccgtcggctg cacgttgacc
    10621 ggatacatcg cgcgcgtgcc gttgtcgtac ggcacttcga gcaagaacgt gatgccgtcc
    10681 ttgaacgtgt cggtgatgcc accggtccag tacacgtcga cgacgcacgg ctccgacgtc
    10741 gatgtccgta gcttcgccat gaacgtcacc gcatccggcg cgacgccaaa cgtgagcgag
    10801 ctctgcggct cggtgaacgg cttgtcgagc gtgtgcatct ggatcgttcc gcaggccgac
    10861 agcacgtagg gcagctgcag cgtcgcgtgc tggtcgaggc acggcgtgcc ctcgggcgtc
    10921 gcatcgaatc cgcagtcgta gtgcggcagc acggcgttcg cgtagccctg ctcgacggcg
    10981 tgctcgatgc acgtatccgc gggaatcgtg tcgtcacagt aggcgcagaa cgcgtccggc
    11041 aagcacgtcg gcgagcgcgc cgtatcgagc ccgcacgtcg tgtcgctcgt gacgccatcg
    11101 gcgcacggcg agcggccgat cacgcaggtg ccgtcgaaca tcggcgtcat gctcgagacg
    11161 cagctgccgt tcggcttgta ctggtaccag aagtcgctgc actccttctc gggcgggatt
    11221 ccgtcgcaat cgggatccgt cttcggcacc agatagtcag tgcgccacgc cgcgccgtcc
    11281 cacttctgat agacgagaca gccggtcggt tcggcgagag ccgacgtcgg cgcgcgattc
    11341 cacacgagcc cgcgccagtt gtgatcggtc ggcggcggcg tgtcgacgtc gttgatcgcg
    11401 acgtcggctg gatggaggtc catcttccag atcacgacct cggaggtcgg gatgaacgtg
    11461 ttgtcctcgg gctgcagcgc tttgatggcg gtgaccttgt caccgtcgta gccgacgaac
    11521 gtgatccacg caggatcctc gaagcccggt tttgcatcga gatggatcac gaagcgatcc
    11581 ggacgcggct ccgcccggat cagcgcttca tcgcgcaaga agtagatgcc gccgctcgtg
    11641 cgctctttgt cgttccacgc gacgccgttc gggcagtcgc cgcattggtc gtatgcgatc
    11701 cacagctcga cgcgatccac cttcacgccg tccggcgcat agatgtcgat gtcgacgccg
    11761 gtgccactgt tgcagccggc gagaacgtaa gccacgagca gcgcacccca gcgcatcacg
    11821 tccaaggtat cacgcgcacc gctcagggcg ggaacaccgg tgtattgacg cactggtcga
    11881 cgagctcgct gaggtcccac gaccacgtcg cctggcaggc gatctgcgca tcgcatccga
    11941 cattgagcgg gtcgacatcg aacacgatcg gcaccgcaac gccgcgaccg ttgtcgagct
    12001 ccgctgccac gaggccggcg aacgtgtgac gtaggcccgg ggaacccgac accacgatct
    12061 cgaagttgca gttgggctgc aggttcttca cgtcgacctt cagcgtcgcg cccgcgtcgg
    12121 tgaattggat gctgttgttc caatcgagac cgatgctcgt gatctggggg aactcgccgc
    12181 tggtcttgca gaagtgcccg ccgatattcg ccggtggctg cgagagcgcc ttgatcgggt
    12241 tcggacacaa gccgccggcc gcgtcgtagt acagcttgca gtggatgtgc gacgggtact
    12301 gcgcggcgag cggcgtgata tcgcgcgcgc agtcccactg gttgccggtc atgttcgtgc
    12361 agcgaccgca caccgacttt ggcatgcaat aggcgctcgg cgccgtgcag ccgttctcgc
    12421 ggccgacgcc atcgcggcag ggcggtccgc cgagcacgca ggcgtcgttc gtcgtgccat
    12481 cggtcgagac gactcgctcg gtgacgaggc aggctgtatc cgaaagttgc ggccgcgcat
    12541 agctcatgaa gaagttcggc tgacattcct tcgcatcgcc ggtgcgccaa ccgtcgcaat
    12601 caggatcgag gtcggtcacg acggcgtcgg cgctcgtcgt gcgcttgtcg aacagcgcga
    12661 cgcacgtctt gccggcctcg ggtgagccgg cagtcgactg ccagacgtcg aggaacagcg
    12721 gagacgcagt gttcgcattg ttcgcgatcg gctcgagcac gaggtcgtaa cgcgcgatga
    12781 actcgcgcgg gacttcgatc gcatgtgcaa cggccgcgga gaccggcgtg tcaccggcat
    12841 agccgatcgc gatcaccacg ccgagcttgt cgccgccctg gaactgaaag acgaccggct
    12901 tgcccgcgct cacttcgcga acgtcgagct cgttgtaatt gtcgcgcacc cacgccgacg
    12961 acttcgggaa cggtgtgctg tgctgcggcg gcatgatcga ttcggtgtac gcgtcgccga
    13021 ggccgacgta gagcgcgacc ttcgtgaccg tgtcgttctt cggctcgacg acaaactcga
    13081 cgccgccacg cgcgcagctc gcgatcacga gcacgagcaa cgcgaccagc cctctcatgt
    13141 gatcagcata cagtgccgac ctattctcga agcaaatatc gaagtgacgt gggccgcagc
    13201 tagaaaatgg cccgcgatcg tcgcgccctc gacgattacg aaatggctat tcgtgcgcgg
    13261 gcggcacgcg gtcttcgcgt cgcaccgcaa gtccaagaag tcgttgcatc accgatcgtc
    13321 aagtgggtcg gcggcaagac gaagctcctc cctgatctgt tgtcccgcat gcccgaacgg
    13381 tacggccgct attacgagcc gtttgccggc ggcgcggcgc tgttctttcg gctcgccccc
    13441 aagcgcgcgg tgctcgccga ttcgaacccc gacctgatcg gcctctacac cgcggtcgcg
    13501 aacgacgtga acgcggtcat tcgtcggctg cagggccatc gcgacgcgca cgacgaagcg
    13561 cactactacg acatgcgcac gcgctggaac gatcgcgacc acgagtggtc gaccccggag
    13621 cgcgcggccg cgttcatcta tctgaacaag acttgcttca atggcctgtg gcgcgtgaac
    13681 cggtcgggcg cgttcaccgt gccgatcggt cgctataccg atccgccgat ctgcgtgccc
    13741 gacgcgctgc gcgctgccca tcacgtgttg tcacgcgccg agatccgccg cgcggactat
    13801 cgcaccgcgg tgcgcgacgc gaaggccggc gacttcctct acttcgatcc gccgtacgac
    13861 ccggtcacgc cgaccgcgaa cttcacgagc tacaccgcgg actcgttcgg cccggacgag
    13921 caacgcgcgc tcgccgacac cgcacgcgag ctcgtcgccc gcggctgtca ggtcatgctg
    13981 tcgaacagcg acacgccgtt cattcggtcg atctacaagg gcttccgcgt cgaccgcgtg
    14041 aagtgcgcgc gggcgatcaa ctccaacgcc gccaagcgtg gcgacgtcga cgagctgatc
    14101 atcactggcg gttactaaac cagcacttac gaaacggggt ctgaccggtc tgaccccttt
    14161 tccggctacg ccagcgcttg cgcttcggcg tactgctgcg tgctcggatc gtagtcgagc
    14221 aacgtgccga ccacgccggc agagcgcgcg atgcgcatga tgtcgtcgcg gtttcccttg
    14281 acgaccatgc gcggttccgc gccttcgaga gaagaggacg atacgaactg gcgagccttg
    14341 acgacgttct tgaacacgag caggtaggtc tgctcgttgc cggcacgcgc catcacgggc
    14401 acgcgaccag ccgggtcttc cgcagtcagc tcaccttcgt tcgacacaag gaggacccag
    14461 aggccatcga gggtcggaga ggaggtttgg agcataagtt ggcgcgggat agatgctcgg
    14521 ttttcctcga tatgtcaaga acctttgtta ccgggtctcg gacgatgacg tatgctgcgt
    14581 cagcccgatg acacgcaccc atcttccggt tctccaggcc accgctttgc ggccgccgag
    14641 ctcgggaaat gtcgtgccgg cggatcccct cgtggacgga tttgcgcggc gcattcgcta
    14701 ccttcgcgtt tctgtcaccg accgctgcaa ctaccgttgc acgtattgca tgccggagac
    14761 actggaagac cgcatgaact tccagccgcg cgccgcgctg ctgacgttcg aagagctgga
    14821 acagatcgtt gcggtgttcg cacggctcgg cgtgcgcaag atccggctga ctggcggcga
    14881 gcccacggtg cgccacggca tcgtcgatct cgtgcgccgc atgacggcag tgccgggcat
    14941 cgagcaggtg gtgatgacga gcaacggtca cttgctcgcc gagctcgccg aaccgctgcg
    15001 cgcggccggg ctatccgcga tcaacatctc cgtcgacacg ctcgacgccg atcgcttccg
    15061 cacgatcacc agccgcggcg agctgtcacg cgtgctcgcc ggcatcgacg ctgcggtcgc
    15121 ggccggcctg cgcgtgaaga cgaacgcggt cgcgttgcgc ggcatcaacg agcacgagct
    15181 cgtcgcgctc tgcgaggacg cgtggcgccg cggcgcgacg ccgcgcttca tcgagcacat
    15241 gccgatgtcc gacggtgcgc tctacgaggc cgcgcagggg atctcggccg cggcgattcg
    15301 ccgcacggtc gaagccgcgc tcggctcgct cgtggccgcc gacgaccgcc caccgaaaat
    15361 ggagccggcc ggattcactc cggcgggcgg ggtcgacgcc ggcccggcgc gctactgggc
    15421 actcgcagcc gacccgacca agcaactcgg catcatctcc gcgatgaccg agcacttctg
    15481 tgatgactgc aatcggttgc gcctgacggc cactggcgag ctgcacgcct gcctggggca
    15541 tgatgacgca gtcagcctcc gcgacgttct ccggggtggg ggctcagacg ccgacctcgt
    15601 tcacgcaatc gccggggctg tacagggcaa acgcgcgggg catgttttcg aggctaaggc
    15661 accggcgaaa cacatgatcg ggattggtgg ctaacgttcg gaatgccggc tgaccgtgct
    15721 acggttggtc gggaaacggg acgccgggct taccgcgaaa gcggagcacc cccaatgaag
    15781 caaggaacgc tcgccaaact cgccgtcacc atagccgtcg tcgctggcgg cgctgggttc
    15841 atggtctatt cgtcgaccaa gcacgcgcag cactacgaga tggtcgacgc gttgctcgcc
    15901 aagggcttcg accagttcaa ggacaaggag atcaaggtcc acggctacgt cgaggcgggc
    15961 accatcgcca aggcgaccgt gaaccaagag acgcggcaca tgttcgtgct gcagaagaac
    16021 ggcaagaaga tccgcgtgtt cgtcagcgga ccgacgccgg atacgttcaa ggatgccagc
    16081 gaggtcgtcg ccaccggccg cctcgttccc gccaccaaca tgcaggcggt cgctgatgcg
    16141 atctgcgcga agccggacag caagctgccc ggcgagtgca aggacggcgc gaaggtgccg
    16201 gccgacgccg agcagacgat ggtcgtcgag gcgaccgagc tgatggcgaa atgcccctcg
    16261 aagtacaaag gcgccgactc gaaccggatc gacactacct acaagtgatc ggccaccacc
    16321 atgactgatc acagcgttcc gttttcaccg atcgcgctgg taggcacgat cgcccttctg
    16381 accgcctacg cggtcgccgc ctggtgtgtt gctgctggca tcgccggcaa cgcgcgcaag
    16441 agccggcgac tggtcaactc cgccgtctac ggcctgtatg gcttcgcggc gctcgtcggc
    16501 cttgcgtcgg cgctcatcat ctacgcgttc gtcacgcacg acttctcgat caagtacgtc
    16561 gcggcgacga gcgatacgac gatgtcgacc tggtacaagg tcaccgcgtt ctggggcggc
    16621 ctcgacggct cactgctgtt ctgggtcctc gtgctcgcgc tgttctcgac gatcgcgatc
    16681 gcggtcaacg cgaagcgaca tcgcgacatg atcgggttcg tcgtcggtac gatcatggtg
    16741 gtgcaggtgt tcttcctgtc gctcctcatc ttcacgaaga acccgttctc gacgtacctc
    16801 accacgccgc ctgccgatgg ccgtggcttg aacccgctgc tccagaacta ctggatggtg
    16861 atccacccgc cgagcctgta cgtcggcttc gtcgcggcga cgattccatt ctcgttcggc
    16921 atcggcgcgc tcgcgtctgg ccgcctcgac gacatgtggc tcggctcggt gcgcgtctgg
    16981 gtgctgatct gcttcggctt cctctcgctc ggcctcatcc tcggcggtcg ctgggcgtat
    17041 gaagagctcg gctggggcgg ctactgggcg tgggacccgg tcgagaacgc cggcttcatg
    17101 ccgtggttct gcgcgaccgc gttcctgcac tccgcgatca ttcaagagca gcgcggaatg
    17161 atgaagacgt ggaacctcgt cctcgtcgtc gtcacgttct tcctcacgat cttcggcacg
    17221 ttcatgaccc gctcgggcgc ggtgcagtcg gtgcacgcgt tcggtgagga caacgagctc
    17281 gcgatcaact tcatcgtgtt catggcgctg atcctgatcg tgtcgatcgg gctcatcgtc
    17341 tatcgctcga gcaagctctc gacgaagacc gagtttgact cgttctactc gcgcgagttc
    17401 gcgttcctgc tcaacaactg gatcctgctc gcgtgcgcgt tcttcgtcct gttcgcgacg
    17461 atgttcccga cgatctccga ggcgatcgat ggctcgcgcg tctcggtcgg ccccgagttc
    17521 ttcaacaagt ggatgacgcc attcgggctg tcactgttgt tcctcgccgg tgccgcgccg
    17581 ctactcgcct ggcgcaagac gacgcgcgaa cggttgtgga accagttcat gttcccgacc
    17641 ggcctgatgg ccgcgacgat cgtcggtctt ttgatcgcgt tcccgcagac gaaagagacc
    17701 agctcgatct tcgccgacaa gctggagatt ccggtctcgc tgatcaactt cggtctcatc
    17761 gcgttctgcg tcggctcgat cggccaggag ttctggcgcg gcatgctcgt gcgccgcaaa
    17821 cagaccggct ccgatccgat gacgtcgctc atcggcctcg tcctgtcgaa gcgccgcaag
    17881 tacggcggct acatcgtgca cctcggcgtc gccgtcatgt tcttcggctt cgccggcaag
    17941 gcgtacgaca gcatgatcga tcgcaccgtc gacaagcccg gcgcctacaa cgcgtcgaat
    18001 ggcaccggcg actcgtcgtt cacgttcacc gttccgaaga ccgacaaggc cggcaacacg
    18061 atccaggatc cggtcttcgt cggtccgttc tcgaagagcc gctacacgtt cacctacgag
    18121 aacctgttcc acaccagcga cgatcacaag gacgcggtga cggcgcaggt cggcatctac
    18181 gactacggcc gccggatcgg caccgtctac ccggcgaagt gggactacca caagggcgac
    18241 ggtcagatga cgaccgaggt cgccatcaag gtgcgcgtgc tcgaagacgt ctacctggtg
    18301 ctcacgggct tcgacctcga gagcaagcag gcgaacttcc gcgtctactt gaacccgctc
    18361 atcctctggg tgtgggtcgg cttcttgatc ctcgcgttcg gcacgctcgt gtgtctgatc
    18421 ccgcagcgcg tcgtcgacct cctgcagtgg aagccgaaga cgcggctcgg ccgtgccgcc
    18481 gatgtcagca tcgtcgcgag cgtgtgcttc gcggtcgtca tgggcatcgc gagccaggcg
    18541 cacgcgcagg gcgaggagca gcagagcagc ggtggcgagc acgtgccggc gggcatgggc
    18601 atgggcgacg cgggcgcagg ctacg
//
LOCUS       DASUFX010000023         9145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1115504, whole genome shotgun sequence.
ACCESSION   DASUFX010000023 DASUFX010000000
VERSION     DASUFX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115504"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFV99_00975"
     CDS             <1..1049
                     /locus_tag="VFV99_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057892.1"
                     /translation="HSSRTGRGGAVRSPYIVSRSYDWLWFLSMPMLSLAIGVLVSGWP
                     SLTTTEHHLGTETDSLVGFFVGTVTHAHLVAVFFRSHGNAQIRKLYPIRFFIVPVVLW
                     LAIVVSPWIAVASTVVATFWDVWHSGAQTFGFARIYERNHGTPPELGRRLDFWLQQLL
                     YAGPILAGATLMDHFDSFSGFKSVGDALFSSVPAAVEGHRRYLTFGVLIAGALLIAAY
                     VIVYWRLAKRGEWKPPWLKLWLVGSTGFVSIYSWGFNSWGQAFLIMNLFHALQYLGLV
                     WAMEKKRICELMRLPNHPRVALSIYVGSVFVYGFAVQALSSDITTLWAITMVVSLMHF
                     WYDAFVWSVRRAQV"
     gene            complement(966..2213)
                     /gene="ubiG"
                     /locus_tag="VFV99_00980"
     CDS             complement(966..2213)
                     /gene="ubiG"
                     /locus_tag="VFV99_00980"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX5057893.1"
                     /translation="MIAACVVPIALQALAMIVDEFGFHRRRELPRWERLGHPLDTLTI
                     ISCLAWLLAVPHDAGYALPFYVGLAIASTLFVTKDEGVHAKLCTAGEHWLHAVLFALH
                     PIVLTAFAWLWWHGERSLLVGQLVVTVAFMTYQLVYWNVLRDRRARAVNNDWYADLGE
                     RWYHAEDTPIALLRAESRHRNPWLADEIENTLGPGCRVLDLGCGAGFLSNYLAARGHH
                     VSGIDMTPENLSVARAHDVTQSATYQIADACDLPFANGSFDVVCAMDLLEHVTAPDRL
                     IAEAGRVLRPGGLFFFHTFNRTKRAHVIVIKGVEWFVANAPKDLHVIELFRTPAEVTT
                     MCENAGFAMMQLRGSRPRFRWPLWRMLATGNVGDDFAFTWTRSLAIGYTGCARRLDLR
                     AAHGPHERVVPEVHERHDHRDRP"
     gene            complement(2210..3331)
                     /locus_tag="VFV99_00985"
     CDS             complement(2210..3331)
                     /locus_tag="VFV99_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX5057894.1"
                     /translation="MRLAGFVATRPQFQIDQARSLAWLAAAHAEAEATLGKLDDRDRR
                     RFEGKVARVLERCACPPSKLATRGHSVADVERVGFATNEVYDLMVHPHGRPIATRMKL
                     FADIVNEYFLHAYAEEQHAPDDLIHVTCTGYVSPSGAQRVVARKGWGGHTRVTHAYHM
                     GCYAALPAVRMAAGFTAIGARRVDIAHTELSSLHLDPADHRVEQLVVQSLFADGMIRY
                     SMYPDAGPGLRLVTSYEVVIPDSHDSMGWRVSDHGLQMTLSRDVPDRIADALRPFVLE
                     LFKRGGMTLGHMKTSLFAVHPGGPKIIDRVRDVLELEDQQVAASRGVLRDYGNMSSAT
                     LPHIWMRMLLDPNVPRGTMIPSLAFGPGLTVCGALLEKQ"
     gene            complement(3439..4656)
                     /locus_tag="VFV99_00990"
     CDS             complement(3439..4656)
                     /locus_tag="VFV99_00990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5057895.1"
                     /translation="MATPIIAFSKDWQEDPTSNHHVLRELATTRRVLWLNSLATRKPN
                     IKSTRDLGKIRRKLVEFTRGAVNVENDLWVATPLVLPLPQSEVANVINRQILRMTIRA
                     LRAQLGIDKFHLVTFLPNTAPYVGTLGEELAFYYCVDEWATFPGLDREGTLAMERQLL
                     ERVDATFTTSLALRDKKRRYCRNTFLAPHGVDYPKFARAIEDALPVPADLAALPGPRI
                     GFFGTLRDFLDYDLMAYVAKARPSWSLVLIGQEMCDIGPLKGLPNVHLLGQKKHDELP
                     AYCKGFDVGLIPYRIDNDVKFINPLKLREYLSAGLPVVSTNMPEVQPYAHLCHIANTG
                     DEMVAAIEQALFEGGVAARIKRSELMANETWQARVASILRIVDQFEARKAQTSEDDLI
                     GAWPVHADALATG"
     gene            complement(4805..5233)
                     /locus_tag="VFV99_00995"
     CDS             complement(4805..5233)
                     /locus_tag="VFV99_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057896.1"
                     /translation="MSAPAAPSVRPEDLPYSPAQHENEGIDFGARQLKAQTAAVEPLV
                     RECVDKAVIAGQHPTGTAMLTYIVAQRGDKFVIETTSADGEKTTLPQEALVECLHQTA
                     KAMKFEGLPKGAHEIYAMRSVTIEDGKITEYKHVSFSYLR"
     gene            5579..5812
                     /locus_tag="VFV99_01000"
     CDS             5579..5812
                     /locus_tag="VFV99_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057897.1"
                     /translation="MRAVLLVLALTACTEVRQVNRWPNHRKEKDAQIAALEDRIHTLE
                     EHIEALEAVLHKLSQPPAAPPAPPPTAVPSPGS"
     gene            5809..7176
                     /locus_tag="VFV99_01005"
     CDS             5809..7176
                     /locus_tag="VFV99_01005"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEX5057898.1"
                     /translation="MSPEEVEIRRRVLALIKQHGWNSTSFQILEPGFHYWVDGDDACV
                     GYVDTGAAWVVAGAPIAPAERLAEVTARFTAAAAAQGRRVCCFGTESRFQDVVQWSAL
                     RIGDQPVWTPSTWDAVIKESKSLREQLRRSRAKGVTIRALEVAELAVDHPVRVRLDAL
                     IARWLATKEIAPMGFIVQLDPFSFPEEKRYFVAERDGTIVGFLAAIPIYARSGWFFED
                     LLRDPTAPNGTTELLIDGAMRAAASANVDHVTLGLAPLAGEVSPWLRFARRWGRPLYD
                     FEGLHAFKSKLKPRAWDPIYLSYPRKRSGIMAIVDTLTAFARGGLLRFGIQTLLRGPA
                     IVVRALAALLVVWTVLLALPHSARFFPSPAWQWGWVGFDIVLGIALFVLAKRWRHDLA
                     TIVAGVVTADAVATAAQVAFFNVPRVHGITDVVVIAIAIAAPTSAAVLLWHARAHRQV
                     LADHE"
     gene            complement(7167..7439)
                     /locus_tag="VFV99_01010"
     CDS             complement(7167..7439)
                     /locus_tag="VFV99_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057899.1"
                     /translation="MIDKHDHGRTLVVTRTPPKHARPNTSFVATPLDLIQLLESEREG
                     VSTVVLTGNFASMRELTMFLVDQYPALRILSGRPGEEPDTYLPAYS"
     gene            7708..8730
                     /locus_tag="VFV99_01015"
     CDS             7708..8730
                     /locus_tag="VFV99_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057900.1"
                     /translation="MKTFAAITTLVGVGLAVAVASTRRSVERVPTELPATLRETGLYA
                     DWDRKEVAAANRPFTPQYPLWTDAARKRRWIYLPRGAAIDASNPDAWQFPVGTRLWKE
                     FSFGARTETRFMMRTRDGWKFATYVWTADGSDAVRAPNGGPVTAEVAPGVRHQVPAEG
                     ECHACHGNGATPVLGFSALQLSPDRDPNALHSEQPAPGSLDLNALVAAKLVLGLPASA
                     RAPRIPGPPVERAALGYLHGNCGHCHRADGAVASVGMVLAYSVVRPPATHTAIAQPSK
                     FMAPHVRVAPGDAEQSVVIARMRTRSPVEQMPPLGTQLVDEAAVHLIATWIDQLAPSQ
                     PTGESK"
     gene            8727..>9145
                     /locus_tag="VFV99_01020"
     CDS             8727..>9145
                     /locus_tag="VFV99_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057901.1"
                     /translation="MTTKHFITMIAVLAAQAACKSEPAPAKQQSPVERGGYLVMTSAC
                     HDCHTPMKMGDKGPEPDFSRQLSGHPAGMVLPPVPALMGPWVMAAAATNTAWAGPWGV
                     SFTRNLTPDKETGIGNWTKQMFIDTIRNGRHMGVGRPL"
BASE COUNT         1535 a         2947 c         3052 g         1611 t
ORIGIN      
        1 cgcactcgtc gcgcactggc agggggggcg ccgtgcgtag cccgtacatc gtcagccgca
       61 gctacgactg gctgtggttc ttgtcgatgc cgatgctgtc gctcgcgatc ggcgtgctcg
      121 tctccggctg gccatcgctg acgaccaccg agcaccacct cggtaccgag accgactcgc
      181 tcgtcggatt cttcgtcggc acggtgacgc acgcgcactt ggttgccgtg ttctttcgta
      241 gccacggcaa tgcgcagatc cgcaagctct atccgatccg gttcttcatc gtgccggtcg
      301 tgctgtggct cgcgatcgtg gtctcgccgt ggatcgcggt cgcgagcacg gtcgtcgcga
      361 cgttctggga cgtctggcac tcgggcgcgc agacgttcgg cttcgcgcgc atctacgagc
      421 gcaaccacgg cacgccaccc gagctcggcc gcaggctcga cttctggttg cagcagctgc
      481 tctacgccgg accgatcctc gcgggcgcga cgctgatgga tcacttcgac tcgttctccg
      541 gcttcaagtc ggtcggcgac gcgttgttct cgtcggtgcc ggccgccgtc gagggccatc
      601 gccgctacct cacgttcggt gtgctgatcg ccggcgcgct gctgatcgca gcctacgtga
      661 tcgtctactg gcggctcgcc aagcgcggcg agtggaagcc gccgtggctc aagctatggc
      721 tcgtcggctc gacgggcttc gtctcgatct actcgtgggg cttcaactcg tggggacaag
      781 cgttcttgat catgaacctg ttccacgcgc tccagtacct cgggctcgtg tgggcgatgg
      841 agaagaagcg gatctgcgag ctgatgcgac taccgaatca cccgcgcgtc gcattgtcga
      901 tttacgtcgg cagcgtgttc gtctacggct tcgcggtgca ggcgctgagc agtgacatca
      961 ccacgctatg ggcgatcacg atggtcgtgt ctctcatgca cttctggtac gacgcgttcg
     1021 tgtggtccgt gcgccgcgcg caggtctagg cgccgcgcac agccggtgta accgatcgcg
     1081 agcgaccgcg tccacgtgaa cgcgaagtcg tcaccgacgt tgccggtggc gagcatgcgc
     1141 cacagcggcc agcgaaaccg cggcctggag ccgcgcagct gcatcatggc gaagccggcg
     1201 ttctcgcaca tcgtggtgac ctcggctggt gtccggaaca gctcgatgac gtgcaggtcc
     1261 ttcggcgcat tcgcgacgaa ccactcgacg cccttgatca cgatcacgtg ggcgcgcttg
     1321 gtgcggttga acgtatggaa gaagaacagt ccgcccggac ggagcacgcg gccggcttcc
     1381 gcgatcaacc ggtcgggcgc ggtgacgtgc tcgagcagat ccatcgcgca tacgacatcg
     1441 aagctgccgt tggcgaacgg caggtcgcag gcatcggcga tttgatacgt cgcggactgc
     1501 gtgacgtcgt gtgcgcgcgc cacgctcagg ttctcgggcg tcatgtcgat gccgctgacg
     1561 tgatggccgc gcgcggcgag ataattcgac aagaagccgg cgccgcagcc gagatcgagc
     1621 acgcgacatc cgggaccgag ggtgttctcg atctcgtcgg cgagccacgg attgcggtgg
     1681 cgcgattcgg cccggagcag tgcgatcggc gtgtcctcgg cgtggtacca gcgctccccg
     1741 agatcggcgt accagtcgtt gttcacggcg cgcgctcggc gatcgcgcag cacgttccag
     1801 tacactagct gatatgtcat gaacgcgacc gtgaccacga gctgaccgac gagcagcgaa
     1861 cgctcaccgt gccaccacaa ccacgcgaac gccgtcagca cgatcgggtg cagcgcgaac
     1921 agaaccgcgt gcagccagtg ctcgcccgcg gtgcatagct tcgcatgcac accctcgtcc
     1981 ttcgtgacga acagcgtcga cgcgatcgcg aggccgacgt agaacggcag cgcatagcca
     2041 gcgtcgtgcg gcactgcgag caaccacgcg agacacgaga tgatcgtgag cgtgtcgagc
     2101 ggatggccga ggcgctccca gcgcggcagc tcgcgacggc gatgaaatcc gaattcgtcg
     2161 acgatcatcg cgagcgcctg cagcgcgatc ggcacgacgc acgcggcgat cattgcttct
     2221 ccaagagcgc accgcacacg gtgaggcccg gaccgaacgc gaggctcggg atcatcgtcc
     2281 cgcgaggcac gttcggatcg agcagcatcc gcatccagat atgcggcagc gttgccgacg
     2341 acatgttgcc gtagtcgcgc agcacgccac ggctcgctgc gacctgctgg tcctcgagct
     2401 cgagtacgtc gcgcacgcga tcgatgatct tcgggccgcc gggatggacg gcgaacagcg
     2461 acgtcttcat gtggccgagc gtcatgccgc cgcgtttgaa cagctcgagc acgaacggcc
     2521 gcagtgcgtc tgcgatgcga tcagggacat cgcgcgacag cgtcatctgg agcccgtgat
     2581 cggagacgcg ccagcccatc gagtcgtgcg aatcgggaat cacgacctcg tacgacgtga
     2641 cgagacgcag ccccgggcct gcgtcggggt acatcgaata ccggatcatg ccgtcggcga
     2701 acaagctctg gacgacgagc tgctcgacgc ggtgatcggc gggatcgaga tgcagcgagc
     2761 tgagctcggt gtgcgcgatg tcgacgcgtc gcgcgccgat cgcggtgaag ccggccgcca
     2821 tacgcaccgc cggcagcgcc gcataacacc ccatgtgata ggcgtgcgtg acgcgcgtgt
     2881 ggccgcccca gcctttgcgc gcgacgacgc gctgggcgcc gctcggcgac acgtagccgg
     2941 tgcacgtgac gtgaatcaga tcgtcggggg cgtgctgctc ctcggcgtac gcgtgaagga
     3001 agtactcgtt gacgatgtct gcgaacagct tcatccgcgt cgcgatcggg cggccgtgcg
     3061 gatgaaccat cagatcgtag acctcgttgg tcgcgaaccc gacgcgctcg acatcggcta
     3121 ccgagtgacc acgcgtggcg agtttgctcg gcggacatgc gcagcgctcg aggacgcggg
     3181 cgaccttgcc ctcgaagcgc cggcggtctc ggtcgtcgag cttgccgagg gttgcttccg
     3241 cctcggcgtg cgccgctgcg agccacgcga ggctgcgtgc ctggtcgatc tgaaactgag
     3301 gtctagtggc gacgaatcca gcgagccgca tgcgaacgcg cgcgagtgca gctttcgatc
     3361 cgcgacggag cgagcggagg tagccggtta gctacaccgc atcgtagcgt ccgagcacgc
     3421 tgtggcggga gcgtcccact aacccgtcgc gagcgcgtcg gcgtgaacgg gccacgcgcc
     3481 gatcaggtcg tcctccgacg tctgcgcctt gcgggcctcg aactgatcga cgatccgcag
     3541 gatcgatgcg acccgagctt gccaggtctc gtttgccatg agctccgatc gcttgatgcg
     3601 cgccgcgacg ccaccttcga acagcgcctg ctcgatcgct gcgaccatct cgtcaccggt
     3661 gttcgcgatg tggcagaggt gagcgtacgg ctggacctcc ggcatgttcg tcgagaccac
     3721 cggcaagccc gccgacaggt actcgcgcag cttgagcggg ttgatgaact tgacgtcgtt
     3781 gtcgatgcga tacggaatca gaccgacgtc gaagcctttg cagtacgccg gcagctcgtc
     3841 gtgcttcttc tggccgagca ggtggacgtt cggcagtccc ttgagcgggc cgatgtcaca
     3901 catctcctgg ccgatcaaca cgagcgacca gctcgggcgc gccttcgcga cgtacgccat
     3961 cagatcgtag tcgaggaaat cgcgcagcgt gccgaagaat ccgatccgcg ggccgggtag
     4021 ggcagcgagg tcggccggca ctggtaacgc gtcttcgatc gcgcgcgcga acttcgggta
     4081 gtcgacgccg tgcggagcga ggaatgtatt gcggcagtag cgacgcttct tgtcgcgcag
     4141 cgcgaggctc gtcgtgaacg tcgcgtcgac gcgctcgagc agctggcgct ccatcgccag
     4201 tgtgccctcg cgatcgaggc cgggaaacgt cgcccattcg tcgacgcagt agtagaacgc
     4261 gagctcttcg ccgagtgtgc cgacatacgg cgcggtgttc ggcaggaacg tgaccagatg
     4321 aaacttgtcg atgccgagct gcgcacgcaa cgcgcggatc gtcattcgca ggatctggcg
     4381 gttgatcacg ttcgcgactt cgctctgcgg cagcggcagc acgagtggcg tcgcgaccca
     4441 cagatcattc tcgacgttga cggcgccgcg cgtgaactcg actagcttgc ggcgaatctt
     4501 gccgaggtcg cgtgtgcttt tgatgtttgg cttgcgcgtc gcgagcgagt tcagccacaa
     4561 cacgcggcgc gtcgttgcga gctcgcgcag cacgtgatga ttcgatgtcg ggtcctcttg
     4621 ccagtctttc gagaaagcga tgatcggcgt agccatgctc gaaacatgag tcaggcatcg
     4681 tcgtaatcca gacgcgcaca ggtgagagtg gctccagaca aaacgcacgt tccgtccaag
     4741 cacacgcgcc caacaaactt ctcttcggga aaatcgtttc ggaaaagttc ctaaggggcg
     4801 ccggctaccg aaggtacgag aatgacacgt gcttgtactc ggtaattttc ccgtcctcga
     4861 tcgtcacact gcgcatggcg tagatctcgt gggcgccctt cggcagacct tcgaacttca
     4921 tcgccttcgc ggtctggtgc aggcattcaa ccaacgcttc ttgtggcagc gtcgtcttct
     4981 cgccatcggc gctcgtcgtc tcgatcacga acttgtcgcc acgctgcgcg acgatatacg
     5041 tgagcatcgc ggtgcccgtc ggatgctggc cggcgatcac cgccttgtcg acacactcgc
     5101 gcacgagcgg ctcgacggcg gcggtctgcg ccttcagttg ccgcgcacca aagtcgatgc
     5161 cttcgttctc gtgctgcgcc ggtgaatagg gcaggtcttc ggggcgcacg gacggcgcag
     5221 ccggcgcgct catcgctggg cgagacggac gagcggcgga gctgggcgcg gcggccgagc
     5281 gagcggtgcc cgaggtagtt cccggccctg gcgccaccgc ggggctgtgt ccagacggcc
     5341 tcgcgatcga ttcggtctgt tgcgcgggcg cggaatcggt gcgcgtcgag tgccacaacc
     5401 cgacgagcgc gaggagcagg ccagcggcac ctgcaaccat caacaagctc gaccgacgcg
     5461 acatgcggtc cggctacctt agcaggcaac gattgccgac cgagcaagtc ttgccggcga
     5521 gatcggcgaa agtcacatac ttcgcggtgg cgtgggcgct gcagtacgct ggcaaccaat
     5581 gcgtgcagtc cttctcgtgc ttgcgctcac agcgtgtacc gaagttcgtc aggtcaaccg
     5641 gtggccgaat caccggaaag aaaaggacgc gcagatcgcg gcactcgagg acaggattca
     5701 cacgctcgag gaacacatcg aggcactaga ggcggtgctg cacaagttgt cgcagccgcc
     5761 cgccgcacca cctgcgccgc cgccgactgc tgtaccttcg ccgggctcgt gagccccgaa
     5821 gaagtcgaga tccgccggcg cgtgctcgcg ctgatcaagc agcacggctg gaactcgact
     5881 tcgtttcaga ttctcgagcc cggctttcac tactgggtcg acggcgacga tgcatgcgtc
     5941 ggctacgtcg acaccggtgc agcgtgggtc gtcgcaggcg cgccgatcgc gcccgcggag
     6001 cggctcgccg aggtgaccgc acgattcacc gccgcggctg ctgcccaggg gcgtcgcgtg
     6061 tgttgcttcg gtaccgagtc gcggttccag gatgtcgtgc agtggtcggc gctgcgcatc
     6121 ggcgatcagc cggtgtggac gcccagcacg tgggatgcgg tcatcaagga gagcaagagc
     6181 ctgcgcgagc agctgaggcg gtcgcgcgcg aaaggcgtta cgattcgtgc gctcgaggtt
     6241 gccgagctcg cggtcgatca cccggtgcgt gtgcggctcg atgcgttgat cgcgcgctgg
     6301 ctcgcgacga aagagattgc gccgatgggc ttcatcgtgc agctcgatcc gttctcgttc
     6361 cccgaggaga agcgttactt cgtcgccgaa cgcgacggca cgatcgtcgg gtttctcgcg
     6421 gcgattccga tctacgcgcg ttcgggctgg ttcttcgaag atctgctgcg cgatccgacc
     6481 gcgccgaacg gaacgaccga gttattgatc gatggcgcga tgcgcgcggc tgccagtgcg
     6541 aacgtcgatc acgtgacgct cggtctggca ccactcgccg gcgaagtgtc accgtggctg
     6601 cggttcgcgc gccgctgggg ccggccactc tacgacttcg aaggtctgca tgcgttcaag
     6661 tcgaagctga agccacgcgc gtgggatccg atctatctct cgtacccgcg caagcggagc
     6721 ggcatcatgg cgatcgtcga cacgctgacc gcattcgcgc gcggcggctt gttgcgcttc
     6781 gggatccaga cgctgttgcg cgggcccgca atcgtcgtcc gtgcactcgc cgcgctactc
     6841 gtcgtgtgga ccgtgttgct ggcgttgccg cacagcgcgc ggttttttcc atcacccgcc
     6901 tggcaatggg gctgggtcgg cttcgacatc gtgctcggca tcgcgctgtt cgtgctcgcg
     6961 aagcgctggc gtcacgatct agcgacgatc gtggcaggtg tcgtcactgc cgatgcggtg
     7021 gcgaccgccg cgcaagttgc gttcttcaac gtgccgcgcg tgcacggcat cacggacgtc
     7081 gtcgtgatcg cgatcgcgat cgcagcgcca acgagcgcgg cggtgctgct ctggcatgcg
     7141 cgcgcgcatc gccaggtact cgcggatcac gagtaagcgg gtaaatacgt atcgggctct
     7201 tcgccggggc ggccggagag gatgcgtagt gcggggtatt gatcgaccag gaacatcgtc
     7261 agctcgcgca tcgacgcgaa gtttccagtc agcacgaccg tcgagactcc ctcgcgctcg
     7321 gattcgagca gctggatgag atcgagcggc gtcgcgacga acgacgtgtt cggtcgcgcg
     7381 tgcttcggtg gcgttcgcgt aacgacaagc gttcggccgt ggtcgtgttt atcgatcatc
     7441 ccgggccgtt gcgttatgca acacgggcac cagcgacgac acctgctcgg ttcgtatgca
     7501 aggcgacgga gtgccaagcg agttggctgc tcccgtacgt ttcgcgagcg cgaccgtgcg
     7561 agcacgccac gggcgaatgc gctcgcattg ccaagtgcgc tcgatccgct cgttagggtt
     7621 cgtttaggaa acgttcagcg acaacttagg aaaagctcag cgagtactta ggaacgcgaa
     7681 tcttcaggag tcggcagatt ggtcgacatg aagacgttcg cagctatcac gacactggtt
     7741 ggtgtcgggc tcgccgtcgc ggtcgccagc acccgccgct cggtcgagcg cgtgcctacc
     7801 gagttgcccg ctacgctccg cgagaccggt ctgtacgcgg actgggatcg caaagaggta
     7861 gcggcggcga atcgcccctt cactccgcag tacccgctat ggacggacgc cgctcgcaag
     7921 cgccgctgga tctatctgcc gcgcggtgct gcgatcgacg cgtcgaaccc cgacgcgtgg
     7981 cagttcccgg tcggcacgcg gctgtggaaa gagttctcct tcggcgcgcg cacggagacg
     8041 cggttcatga tgcgcactcg cgacggctgg aagttcgcga cgtacgtatg gactgccgac
     8101 ggttctgacg ccgtgcgcgc gccgaatgga ggcccggtca ctgccgaggt ggcgccgggc
     8161 gtgcggcatc aggttccggc cgaaggcgag tgccacgcat gtcacggcaa cggcgcaacg
     8221 ccggtactcg gcttctcggc gctccagctc agtcccgatc gcgatccgaa cgcgctgcac
     8281 agcgagcagc cagcgcccgg ctcgctcgat ctgaatgcgc tcgtggccgc gaagctcgtg
     8341 ctcggattgc cggcgagcgc acgcgcgccg cggattccgg gaccgccagt cgagcgcgcc
     8401 gcactcggtt acttgcatgg caactgcggg cactgccatc gcgccgacgg tgcggtcgcg
     8461 agcgtcggca tggtgctcgc gtattcggtc gttcggccgc ccgcgacgca caccgcgatc
     8521 gctcagccga gcaagttcat ggcaccgcac gtgcgcgtag cgccgggtga tgccgagcag
     8581 agcgtggtga tcgcacgcat gcgtactcgt tcgcccgtcg agcaaatgcc gccgctcggc
     8641 acccagctcg tcgacgaagc agccgttcat ctcatcgcga cctggatcga ccagctcgca
     8701 ccttcgcaac caacaggaga atcgaaatga ctacgaagca cttcatcacc atgatcgcgg
     8761 tcctcgccgc ccaggccgcc tgcaagagcg agccggcacc ggccaagcaa caatcccccg
     8821 tcgagcgcgg cggctacctc gtcatgacga gcgcctgcca cgactgccac acgccgatga
     8881 agatgggcga caagggaccc gagccggact tcagccgcca gctctcggga catccggccg
     8941 gcatggtgtt gccgccagtg ccggcgctca tggggccatg ggtcatggcc gcggcggcga
     9001 cgaacaccgc atgggctgga ccgtggggcg tgtcgttcac gcgcaacctg acgcccgaca
     9061 aggagaccgg catcggcaac tggacgaagc agatgttcat cgacacgatc cgcaatggtc
     9121 gtcacatggg cgtcggccgg ccact
//
LOCUS       DASUFX010000024         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_421050,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000024 DASUFX010000000
VERSION     DASUFX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421050"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFV99_01025"
     CDS             complement(<1..674)
                     /locus_tag="VFV99_01025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057902.1"
                     /translation="MSGDKGEQPTIKVAITGEEVGADRPEPSPDTMMGGRYRLGRRIG
                     KGGMGEVMAARDTQIARDVAIKRMRAATPSEKAISRFLREAMVQGRLEHPAIVPVHEI
                     GRDADGLPYFVMKKLSGTSLAKILDDDAPITLQRVLRAFADVCLAIEFAHVRGIVHRD
                     LKPDNIMLGDYGEVYILDWGVAKVVGEEDSDFADVGSGSGEFVATRVGTAVGTPGYMA
                     PEAVRG"
     gene            831..1994
                     /locus_tag="VFV99_01030"
     CDS             831..1994
                     /locus_tag="VFV99_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057903.1"
                     /translation="MTWLRLLCAVSLVGCVDNAGAPAGDDIDLAGIDTSAIGADKAQP
                     SSWQAAATLHDGSHLFDFAREGTRHVHSMWVAGSNNNRVPLTIAAHTDIDHDVRIAVL
                     GPLADDGTRAVLAADGYATRKQTVSVSVDVAQPGEHLVVVGSYNLASDTFYDLAAACV
                     GPTCSVSRVDALATPKDGALVGDQDLVSMLLGDALVGYGDIEVELWASPPLQPGSAQL
                     VGTSEASGSQINALVPTSVQAGDDLRLVVREAGGGRVLDTGVMTRFAPQPAAFARLDS
                     IGYSDGTTVQIAGVVGFFEGVADMRMYSETHHREIAQQVLHVDRPGQIGNGLNGFDAT
                     FRPDAAVAAENGDLVSIGFINGNGDYRRLGCFEYCNPLSTISSCTGGPRACQL"
     gene            2002..2388
                     /locus_tag="VFV99_01035"
     CDS             2002..2388
                     /locus_tag="VFV99_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX5057904.1"
                     /translation="MNPYEELGGSEHVRAIVERFYDLMHEREPALARLHSCTPEGKVD
                     RGSRDRFALFLIGWLGGPDDYVMQHGHPRLRMRHGRVAVNIAMRDAWLRCMSTALDEH
                     GVTGHVREFLDARFAEVADFMRNVDG"
     gene            complement(2389..3693)
                     /locus_tag="VFV99_01040"
     CDS             complement(2389..3693)
                     /locus_tag="VFV99_01040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057905.1"
                     /translation="MLGAGGMGEVYLAKHIALGMKRAVKVLRTDLRARQESRDRFSRE
                     AQVLARLQHNSIVQLIEFGALDNGWPFLAMEYIEGPGLDELVAFEALPMASALVVLEQ
                     LAVALQYAHSLGVIHRDLKPSNILVRSGDLRQVKVIDFGLARLVDTDTLKKLTADGQP
                     LGSPAYMAPEQVDGGEVSAAADVYALGGIAYMLLSGKPPFERKTSIQLMAAHKSDEPP
                     RISSRCAEISEFLDSLFYACLAKDPAQRPASDELASHLSRITRGTELPRRPSAPAPLQ
                     LDDGPRVEVVASPWATRSVPTEILETAPPDGAGAALANQIIAMIGEIANYLSTSDPEL
                     TSLLRLEASIREQLSDVERDSAAVNLQLEQSSAMTVARLTQQQRELIERRRTLHAQQK
                     PLQKRMIEVVETHRRYADGPVKFLFEQIDRALEHLDDMRSKS"
     gene            complement(3886..>4586)
                     /locus_tag="VFV99_01045"
     CDS             complement(3886..>4586)
                     /locus_tag="VFV99_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5057906.1"
                     /translation="RVMSYNVNFGVAGDPSTIDAIANAQPDVVFLQETNEVWQRALVA
                     RLGTSYPHQRFTHPETWPAGGMGLLSKYPVIALQHLDSVGGYFFAWRVVLDTKLGRIQ
                     VLNVHLRPPMSDGGSWVVGYWSTRSDRLREIENHIAALDPKLPTIILGDFNEEGDGRA
                     VELLKDRGYGDALPQFVGKTRTWEWPVGSITLRFQLDHIMHDGYFVPVTGAIVEGGHS
                     DHKAIWVDFERIDP"
BASE COUNT          783 a         1506 c         1458 g          839 t
ORIGIN      
        1 tggccacgca ccgcttcggg agccatgtag cccggcgtac cgaccgcagt accgacgcgc
       61 gtcgccacga actcaccgct gccgctgccg acgtctgcga agtcgctgtc ctcttcaccg
      121 acgaccttcg cgacacccca gtcgagaatg taaacctcgc cgtagtcgcc gagcatgatg
      181 ttgtcgggct tgaggtcgcg gtgaacgatg ccgcgcacgt gcgcgaactc gatcgcgagg
      241 cagacgtccg cgaacgcgcg cagtacccgc tggagcgtga tcggtgcatc gtcgtcgagg
      301 atcttggcga gcgacgtgcc cgagagcttc ttcatcacga agtacggcag accgtcggcg
      361 tcgcgcccga tctcgtgcac cggcacgatc gccgggtgct cgaggcggcc ctgcaccatc
      421 gcttcgcgca gaaatcgcga gattgccttc tcgctcggcg tagccgcacg catccgcttg
      481 atcgccacgt cgcgcgcaat ctgcgtatcg cgtgcggcca tgacttcacc catgccgcct
      541 ttgccgatgc gacgaccgag tcggtaacgg ccacccatca tcgtgtccgg actcggctcg
      601 ggacgatctg cgcctacttc ttcgccggtg atcgcgacct tgatagtcgg ctgttcaccc
      661 ttgtcgccgc tcatacccac caggataacc cgaaccgatt tgtgcacgcg cgtgcgcgac
      721 atggtgacca gtcgagtagc cgcgagacgc ggacctcgcg gcacatgtca tgcagtcgtg
      781 cgcgaacggt gatgcaaagc gattcgaatt ggtctaccta cccgcaccgt atgacgtggc
      841 tcaggttgct ctgtgcggtc tctctcgtag ggtgtgtcga taacgctggc gcgccagcgg
      901 gcgacgacat cgatctggcg ggcatcgata cgtcagcgat cggcgccgac aaggcccaac
      961 cgtccagctg gcaagcagcg gcgacgttgc acgacggcag ccacctgttc gactttgccc
     1021 gcgaaggaac gcgccacgtc cattcgatgt gggtcgcggg cagcaacaac aaccgcgtgc
     1081 cgctgacgat cgcggcgcac accgatatcg atcacgacgt gcgcatcgct gtgctcggtc
     1141 cgctcgccga cgacggcacg cgcgccgtgc tcgccgccga cggctacgcg acgcgcaagc
     1201 agacggtgtc ggtgtcggtc gacgttgcac agcccggcga gcacctcgtc gttgtcggct
     1261 cgtacaatct cgcaagcgac acgttctacg atctcgccgc cgcgtgtgtc ggtccgacgt
     1321 gcagcgtgtc tcgcgtcgac gcgctcgcga ctccaaagga tggcgcgctg gtcggcgatc
     1381 aagacctcgt gtcgatgctg ctcggcgatg cgctcgtcgg ctacggtgat atcgaagtcg
     1441 agctctgggc gtcgccgccg ctgcagccgg gctcggcgca gctcgtcggt acgagtgaag
     1501 catcgggctc gcagatcaac gcgctcgtgc ccacgtccgt gcaggccggc gatgatctgc
     1561 ggctcgtcgt gcgcgaagcc ggtggcggcc gcgtgctcga caccggtgtg atgacgcgat
     1621 tcgcgccgca accggccgcg ttcgcgcggc tcgacagcat cggatacagc gacggcacga
     1681 ccgtacagat cgccggcgtc gtcgggttct tcgagggcgt cgcggatatg cggatgtatt
     1741 cggagacgca ccatcgcgag atcgcgcagc aggtgctgca tgtcgatcgt ccgggtcaga
     1801 tcggaaatgg tctgaacgga ttcgatgcga cattccgtcc cgacgccgcg gtcgccgcgg
     1861 agaacggcga tctcgtatcg atcgggttca tcaacggcaa cggcgactac cgccggctcg
     1921 ggtgtttcga gtactgcaac ccgctctcga cgatctcgag ctgcacgggc ggaccgcgag
     1981 cttgccagct atagtcgccg cgtgaatccg tacgaggagc ttggtggtag cgagcacgtg
     2041 cgtgcgatcg tcgagcgctt ctacgacctg atgcatgaac gcgagcctgc gctcgcacgg
     2101 ctgcacagct gcacgcccga gggcaaggtc gatcgcggct cgcgcgaccg gttcgcgctg
     2161 ttcttgatcg ggtggctcgg cggtcccgac gactacgtga tgcagcacgg ccatccccgg
     2221 ctgcgcatgc gtcatgggcg ggtcgcggtg aacatcgcga tgcgcgatgc ctggctgcgc
     2281 tgcatgtcca cagcactcga cgaacacggc gtgaccggcc atgtgcgcga gtttctcgac
     2341 gcgcggttcg ccgaggtcgc cgacttcatg cgcaacgtcg acggctgatc acgacttgct
     2401 gcgcatgtcg tcgaggtgtt cgagcgcgcg gtcgatctgt tcgaacagga acttcaccgg
     2461 cccgtccgca tagcgacggt gcgtctcgac gacctcgatc atccgcttct gcagtggctt
     2521 ctgctgcgcg tgcagcgtac gcctgcgctc gatgagctcg cgttgctgtt gggtgagccg
     2581 cgcgacggtc atcgcactcg attgttcgag ctgcaaattg accgcggcgc tgtcgcgttc
     2641 gacgtcggag agctgctcgc gaatgctcgc ttcgagccgc agcaacgacg tcagctcggg
     2701 atcgctcgtc gacaagtagt ttgcgatctc gccgatcatc gcgatgatct gattcgcgag
     2761 cgcagcgccc gcaccatcgg gcggcgccgt ctcgaggatc tcggtcggca cactgcgcgt
     2821 ggcccacggc gatgcgacaa cctcgacgcg agggccatcg tcgagctgca atggtgctgg
     2881 tgcgctcggc cggcgcggca gctccgtacc gcgcgtgatg cggctgaggt gacttgcgag
     2941 ctcgtcgctg gccggccgct gcgccgggtc cttcgcgagg caggcgtaaa atagcgaatc
     3001 gagaaactcc gagatctcgg cgcaccgtga cgagatccga ggcggctcgt cgctcttgtg
     3061 cgcggccatc agctggatcg acgtcttgcg ctcgaacggc ggcttccccg acagcaacat
     3121 gtatgcgatg ccgccgagcg cgtaaacatc ggcagccgcg ctgacctcgc cgccgtcgac
     3181 ttgctcgggc gccatgtacg ccggcgaacc gagcggctga ccatcggcgg tgagtttctt
     3241 cagcgtgtcg gtatcgacca ggcgcgcgag gccgaagtcg atgaccttga cctgccgcag
     3301 atcgcccgag cgcacgagga tgttggacgg cttcagatcg cgatgaatca cgccgagcga
     3361 atgagcgtac tgcaacgcca ctgcgagctg ttcgagcacg acgagcgcgc tcgccatcgg
     3421 cagcgcttcg aaggcaacga gctcgtcgag ccccgggcct tcgatgtact ccatcgcgag
     3481 aaatggccag ccgttgtcta gcgcgccgaa ctcgatgagc tggacgatcg agttgtgctg
     3541 cagccgcgcg agcacctgcg cctcgcggct gaagcgatcg cgcgactcct gacgcgcacg
     3601 cagatcggtg cgcagcacct tgaccgcgcg cttcatgccg agcgcgatgt gcttcgcgag
     3661 gtaaacctcg cccatacctc cggcaccgag cacgcgaatg agctcgtgcc ccgcgacgat
     3721 ctcccccgca agagtgccgc gcctcgatct gggaggctct tccaatgtgc ttacccgttt
     3781 ccccgtgtta gcgaggtact cttcgctact ttcgactttg cagggaggat agccaagtgg
     3841 tcaacggcta tctcgggggt agtcgcggtg tacttggttt gcgcactacg gatcgatgcg
     3901 ctcgaagtcg acccaaattg ctttgtgatc cgagtgtccg ccctcgacga tcgcgcccgt
     3961 caccggcacg aagtagccgt cgtgcatgat gtggtcgagc tggaaccgca gcgtgatgct
     4021 acccaccggc cactcccagg tgcgtgtctt accgacgaac tgcggcagtg catcaccgta
     4081 gccgcgatcc ttcaggagct cgacggcgcg cccgtcgcct tcttcgttga agtcaccaag
     4141 gatgatcgtc ggcaacttcg gatcgagagc cgcaatatgg ttttcgatct cccgcaaccg
     4201 atcgctgcgc gtcgaccagt agccaacaac ccagctgccg ccgtcgctca tcggcggccg
     4261 caagtgcacg ttcaatacct gaattcggcc gagcttcgta tctaggacga cacgccacgc
     4321 gaagaagtaa ccacccacac tatcgaggtg ttgcaacgcg ataacgggat acttcgatag
     4381 caaccccata cctccagctg gccacgtttc aggatgcgtg aaccgctgat gcggatagct
     4441 ggtgccgagc cgcgcaacaa gtgcgcgttg ccagacctcg ttcgtctcct gcaggaacac
     4501 gacatcgggc tgcgcgttcg cgatggcatc gatcgtcgag ggatcacctg caacgccgaa
     4561 gttgacgttg tagctcatca cccgca
//
LOCUS       DASUFX010000025         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_421244,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000025 DASUFX010000000
VERSION     DASUFX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421244"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VFV99_01050"
     CDS             <1..1264
                     /locus_tag="VFV99_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057907.1"
                     /translation="DDRVRTFVAAGVQVIAATDHDTIGDYASTVAQLGLDDRVAVMGG
                     LETTQLIPWLDVPGESVPRVIGHFNFWPLVRDPAAPRAGAPWDELIEPGALFDKMAPL
                     VGDGGMMMLNHPWSDPIFGRDTGYLRAIKFDPRKPIDARSTLMKRPGGQHRNMDWSII
                     EIINGSDMSEIMQSRTLWFSLLAQGFISPGAGNSDSHGMSDDQLGWARNWVQAPTQVA
                     GFDPNAFNTAIRDGRMIAGNGVVVLVEVGPPEGPRRGLGFTPYVPKPGDVVDITVKAA
                     PWIPLNEVRIVTSKGTQIVGEIPREPNPFGSNVLRFQGQFPIDSLVTKDDFLIVEAGM
                     AYPLAADLDNDGVLDTTDNNGDGAVDDADVDPDEDVGPVKPPPDPTAADDQRFLLTQL
                     VSGAWPEGFANPIFIDVDGNGWTPPGLP"
     gene            1261..2115
                     /locus_tag="VFV99_01055"
     CDS             1261..2115
                     /locus_tag="VFV99_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057908.1"
                     /translation="MRAAALLLVVLAPALAIADTDSTTAEFHPAEPEQLVDPCASALV
                     DPIVTPVRDIDLDAQRSACMRRDLFVRVLGHALIDTPGFHGVLGGDLAIGGHLRIRTN
                     HELFAQLRIIDYSFVQNAVNKVTNAGFGPLVLGAATSTWFGDAARAALAFQLELPYTR
                     DEMDTTHVSGALTGLLTSKLRRALFLNARLGALGMRAASMAGSTNRLAFHAGSDAVWH
                     VKTRLALQAGADLSAGWSGFDYVLFRAGIHWRITSGDWRLRAGVGLPVFGSERTNAVL
                     DLALVHGL"
     gene            2186..2536
                     /locus_tag="VFV99_01060"
     CDS             2186..2536
                     /locus_tag="VFV99_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057909.1"
                     /translation="MSADAIARLTSLIQSADTDPSALEREVGDKLADEVGGDLALRWR
                     MGVVKALMAAPPDPDAVREAYGELVDRYRDDDAALAAIKPLGDEIRKRESDGSLPSTL
                     VVRSNRRKTTKQGI"
     gene            2630..>3719
                     /locus_tag="VFV99_01065"
     CDS             2630..>3719
                     /locus_tag="VFV99_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5057910.1"
                     /translation="MSLVVLDGVSLFFGDRMIFDNISLRLGHGDRIGLIGPNGSGKTT
                     LLKVIAGEQEIDDGGVTRANGVRVGWLPQDLAIAGGRSLIDMILSSVPGRAELNDHLA
                     ATETALEQATKDGADEATVLELAGEIGELHERIDHFDRFFGEHEALTILAGLGFAPGD
                     ETRDLAEFSGGWKMRGVLAGLLFQRPDVLLLDEPTNHLDLPSVAWFGDFLKRWNRCFI
                     LISHDREFLNDQISRVVSLELEGVRSYPGNYEKYVAARAEEETILAGKAKNLARERER
                     LTRFVDRFRAQANKARAVQSRIKMLEKMEDVELYGKRGTMRFSFPATSRAVAEPVRIE
                     KLKKAYGDHVVFPGIDLAVKRGEKIGIIG"
BASE COUNT          621 a         1224 c         1255 g          619 t
ORIGIN      
        1 cgacgatcgc gtgcgcacgt tcgtcgcggc cggcgttcag gtgatcgcgg cgaccgatca
       61 cgacacgatc ggtgactacg cgagcaccgt cgcgcagctc ggcctcgacg accgcgtcgc
      121 ggtcatgggt ggcctcgaga caacgcagct gattccgtgg ctggacgtgc ccggcgagtc
      181 ggtgccgcgc gtgatcggtc acttcaactt ctggccgctc gtgcgcgatc cagccgcgcc
      241 gcgcgccggt gcgccgtggg acgagctgat cgagcccggc gcgctgttcg acaagatggc
      301 gccgctcgtc ggcgacggcg gcatgatgat gctgaaccat ccgtggagcg atccgatctt
      361 cgggcgcgac acgggatacc tgcgcgcgat caagttcgat ccgcgtaaac cgatcgatgc
      421 gcgctcgacc ctgatgaagc gtcccggcgg tcagcaccgc aacatggact ggtcgatcat
      481 cgagatcatc aacggcagcg acatgagtga gataatgcag tcgcgcacgt tgtggttctc
      541 gttgctcgcg cagggcttca tctcgcccgg cgccggcaac agcgactcgc acggcatgtc
      601 cgacgatcag ctcggttggg cgcgcaactg ggtacaggca ccgacgcagg tcgcgggttt
      661 cgatccaaac gcgttcaaca cggcgatccg cgatggccgg atgatcgcgg gcaacggcgt
      721 cgtcgtgctc gtcgaggtcg gtccaccgga gggtccgcgg cgcggcctcg gcttcacgcc
      781 gtacgtgccg aagcccggcg acgtcgtcga catcacggtg aaggcagcgc cgtggatccc
      841 gctcaacgag gtgcgcatcg tgacgtcgaa gggcacgcag atcgtcggcg agattccgcg
      901 cgagccgaat ccgttcggct cgaacgtgct ccgctttcag ggccagttcc cgatcgactc
      961 gctcgtcacc aaggatgact tcctgatcgt cgaggccggc atggcgtatc cgctcgcggc
     1021 cgatctcgac aacgacggcg tcctcgacac gaccgacaac aacggcgacg gcgccgtgga
     1081 tgatgcggac gtcgatcccg acgaggatgt cgggccggta aagccgccac ccgatccgac
     1141 cgcggcggac gatcagcggt tcttgctgac gcagctggtg tcgggcgcat ggcccgaggg
     1201 cttcgcgaat ccgatcttca tcgacgtcga tggcaacggc tggacgccgc cggggttgcc
     1261 gtgagagcgg ccgcgctgct gctcgtcgtg ctcgcgcctg cgttggcgat cgccgacacg
     1321 gactcgacga cggccgaatt ccatccggca gagcccgagc agctcgtcga tccatgcgcg
     1381 agcgcgctcg tcgatccgat cgtcacaccg gtgcgcgaca tcgatctcga tgcgcagcgc
     1441 tccgcatgca tgcggcgcga tctgttcgtt cgcgtgctcg gccacgcact gatcgatacg
     1501 cccggctttc acggcgtgct cggcggcgat ctcgcaatcg gtggccacct gcgcatccgc
     1561 acgaaccacg agctgttcgc acagctcaga atcatcgact actcgttcgt ccagaacgcg
     1621 gtcaacaagg tcacgaacgc cggcttcggc ccgctcgttc tcggcgccgc aacgagcacc
     1681 tggtttggcg acgcggcgcg cgctgcactc gcgttccagc tcgagctgcc gtacacgcgc
     1741 gacgagatgg acaccacaca cgtgagcggc gcgctcacgg gactcctcac gagcaagctg
     1801 cgtagggcgc tgttcttgaa cgcgcgactc ggcgcgctcg gcatgcgtgc agcgtcgatg
     1861 gccggcagca cgaatcgact cgcgttccac gccggatccg atgccgtgtg gcacgtgaag
     1921 acgcggctcg cgctgcaggc gggtgctgac ctgtcggcgg gatggtcggg cttcgactac
     1981 gtactgtttc gcgccggcat tcactggcgc atcacgagcg gcgattggcg cctgcgcgcc
     2041 ggcgtcggtc tgccggtgtt cggcagcgag cgcacgaacg cggtgctcga cctcgcgctc
     2101 gtgcacggtt tgtaacccca ccgttagccg atctcacccg aacgagccgc gagcgcgcga
     2161 tcccgagcgt gctacgatcg gagccatgtc ggccgacgcg atcgcgcgcc tgacctcgct
     2221 catccagagc gcggatacgg atccatctgc gctcgagcgc gaggtgggtg acaagctggc
     2281 ggatgaagtc ggcggcgacc tcgcgctgcg ctggcgcatg ggcgtcgtga aggcgctgat
     2341 ggccgcgccg cccgatccgg atgcggtgcg cgaggcgtac ggcgagctcg tcgatcgcta
     2401 tcgcgacgac gatgccgcgc tcgctgcgat caagccgctc ggcgacgaga tccgcaagcg
     2461 cgagtcggat ggctcgctgc cgagcacgct cgtcgtgcgt agcaaccgcc gcaagacgac
     2521 caagcaagga atctgacagg catcaggcct caggcctcag gttccggatc tgacgcgagg
     2581 cctgaagtct gaggtctgag gcctgctatg tgctaagcat tcgccgctca tgagtctggt
     2641 cgtactcgat ggcgtctcgc tgttcttcgg agatcgcatg atcttcgaca acatcagcct
     2701 gcgcctcggt cacggtgacc gcatcggcct gatcgggccg aacggctcgg gcaaaacgac
     2761 gctgctcaaa gtgatcgccg gcgagcaaga gatcgacgac ggcggcgtca cgcgcgcgaa
     2821 cggcgtgcgc gtcggttggc taccgcagga cctcgcgatc gctggcggcc gctcgctgat
     2881 cgacatgatc ctgtcgagcg tcccgggcag ggcagagctc aacgaccacc tcgcggcgac
     2941 cgagaccgcg ctcgagcagg cgacgaagga cggcgcagat gaagcgaccg tcctcgagct
     3001 agctggcgag atcggcgagc tgcacgagcg catcgatcac ttcgatcggt tcttcggcga
     3061 gcacgaggcg ctcacgatcc tcgcgggcct cggctttgcg cccggtgacg agacgcgcga
     3121 tctcgccgag ttctcgggcg gctggaagat gcgtggcgtg ctcgcgggcc tgttgttcca
     3181 gcgccccgac gtcctgttgc tcgacgagcc gacgaaccac ctcgatttgc cgtcggtcgc
     3241 gtggttcggc gactttctca agcgctggaa tcgctgcttc atcttgatca gccacgatcg
     3301 cgagttcttg aacgatcaga tctcgcgcgt cgtcagcctc gagctcgaag gcgtgcgcag
     3361 ctatcccggc aattacgaga agtacgtcgc cgcgcgcgcc gaggaagaga cgattctcgc
     3421 cggcaaagcg aagaacctgg cgcgcgagcg cgagcgcctc acgcggtttg tcgatcggtt
     3481 tcgcgcgcag gcgaacaagg cgcgcgcggt gcagtcgcgc atcaagatgc tcgagaagat
     3541 ggaagacgtc gagctctacg ggaagcgcgg cacgatgcgg ttctcgttcc cggcgacgtc
     3601 ccgcgcggtc gccgagcccg tgcgcatcga gaagctgaag aaggcgtacg gcgatcacgt
     3661 cgtgttcccc ggcatcgatc tcgcggtcaa gcgcggtgag aagatcggca tcatcggcg
//
LOCUS       DASUFX010000026        11521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1950031, whole genome shotgun sequence.
ACCESSION   DASUFX010000026 DASUFX010000000
VERSION     DASUFX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950031"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VFV99_01070"
     CDS             <1..1509
                     /locus_tag="VFV99_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5057911.1"
                     /translation="EAVWYCDAMLAGGKNANGVFTATKDPAIATLVRQVKADIGEFRR
                     KGDARYALFKSGSNTAAVGSTLDTEFDTIFENFLKNADDAALLIDRSIDRSVEGLDAM
                     ESSARTFMVVTIVLGVAMALALGMLIARSISRPLRQAVDAANRVALGHVDVKLEGTTR
                     DETGMLLQAMREMMDANRAMSIAAQDIAAGNLAVKVVPRSDKDALGQALSTMVTNLAA
                     TIGEVRSAAAALSSAAEQVAASSQSLSQGTSEQASAVEETSSTLEEMNASITQNAENS
                     RRTELMSVKGAVDAEESGKTVEETSKAMETIADKVSIIEEIAYQTNLLALNAAIEAAR
                     AGDHGRGFGVVANEVRKLAERSQGSAREITQLAGASVKVAGRSQALLAELVPSIRKTT
                     EVVQEVAAASQEQASGVNQVSKAIAQMDMVTQRNAAAAEELSSTAEELSAQAEALQQL
                     MQYFKLQGTEHQTKTPVIDRRLSKPTLVYPNGKHVGLNGAHAAIPEGEFRAF"
     gene            1517..2026
                     /locus_tag="VFV99_01075"
     CDS             1517..2026
                     /locus_tag="VFV99_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5057912.1"
                     /translation="METKRYLTFYVGDQEYGIDILESRAVVQFPTITRLPWMPASIRG
                     VINMRGSILPVVDLAVLFGCEARPTTNWTCVVVVESAGAPGEQSRLGLVVDSVSQVIE
                     VRGEDLEAVPAFGTRIPTKFLRGIVTAGATFVHVLDLAHVVEHVVHQLQRREALSAVV
                     AAESSEGLQ"
     gene            2023..2898
                     /locus_tag="VFV99_01080"
     CDS             2023..2898
                     /locus_tag="VFV99_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX5057913.1"
                     /translation="MSAAPALAAPALSEREFQLFQKLVLREAGIFLGEHKRALVIGRL
                     SRRLRELGMTSFGVYYDHVTRSGDAELVHLLDAICTNETHFFREPDHFSFLESHALRE
                     FEALAAAGKIPKRLRVWSAACSSGEEPYSLAMTLRARFPRESGWHLEILASDLSTRIL
                     DRARAATWPIEKTSEIPYDYLRRFMLRGTGSQSGKMKAGAEIRSLVEFSRINLNDDNW
                     PVAGKFDLIFCRNVLIYFERTGKAKVARHLLDRLSPHGYLFLGHAETLAGLSDQVRSV
                     SPSVYQLRQRVTEDC"
     gene            2898..3968
                     /locus_tag="VFV99_01085"
     CDS             2898..3968
                     /locus_tag="VFV99_01085"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX5057914.1"
                     /translation="MPQSPARLHVLVVDDSAVVRTALQALLAKDPGITVAVASDPIIA
                     MTKIKMSRPDVIVLDLQMPRMDGLTFLARLMAEQPLPVVVCSSQAGPSTEAALRALML
                     GAVDLIPKPQIGVRDFLEHSVTQFIETIRGAAHARVNRRVMPAAPAASVAVQPRAVSP
                     EYRASDRVVAIGASTGGTEALRQILSAMPANGPAMVIVQHMPAGFTAAFAKQLSETCA
                     IEVKEAADGDLLVNGRALVAPGNRHVHLVRSGSRYAVRVTDGAPVSRHRPSVDVLFRS
                     VAAVAGRNAVGVIMTGMGHDGAEGLRDMREAGAYTLAQDEASCVVFGMPREAIARGGV
                     DRVVSLSHLPAAILQSVQPAGH"
     gene            3985..4518
                     /locus_tag="VFV99_01090"
     CDS             3985..4518
                     /locus_tag="VFV99_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5057915.1"
                     /translation="MSSNPIDVDDRMAERCEYLTFFVRGEEYAVGLRRVREVIAFNKI
                     TRIPHMPPALRGVTGFRGEVVPVLDLAIKFHGIETAVDPKLCASLMLVETVIDGRPRV
                     LGLITEAVGQVLALTDADVVPPPSFGAPIRTEFLAGRGRVGTKFALLLDIDRLLTFDE
                     LLSAQLAEPLAVTDAAV"
     gene            4528..5250
                     /locus_tag="VFV99_01095"
     CDS             4528..5250
                     /locus_tag="VFV99_01095"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5057916.1"
                     /translation="MGRAFEKRKHTIFARMDRVAKQFTKINKDIAIAVKASGPNPEGN
                     PALRRALQNARALNMPKDKVEAAIKKASGQGATNYEIVLYEGYAPHGIALLVETATDN
                     VTRTVANIRHHFKANGGNMGTTGSVAFMFQKQGVFRLNPEGIDRDTLELELIDHGLVA
                     LEDGESDKGEKQLVVRCAFTDFGTMQSALEARNITPISSSSDYAAATQTELPDDKADE
                     VMKLVAAMEEDDDVQNVYTNMA"
     gene            5250..5807
                     /locus_tag="VFV99_01100"
     CDS             5250..5807
                     /locus_tag="VFV99_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5057917.1"
                     /translation="MLIALNKPYLVHCKFTDQQGRPTLAAFGLPPRVYVAGRLDHDSE
                     GLLLLTDDGKLNQRLTDPRWKAPKTYCVQVEGTPTEEALAGLRAGVDLNVGRTLPADA
                     RAIDEPPWLWPRTPPIRVRKTVPDAWIELTIREGRNRQVRRMTAAVGLPTLRLIRVAI
                     GPYSLDGLAPGQWREQVVTSSSARR"
     gene            complement(5782..5913)
                     /locus_tag="VFV99_01105"
     CDS             complement(5782..5913)
                     /locus_tag="VFV99_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057918.1"
                     /translation="MQSDDSGSVPRQNLTFWQRNALTIIIAVMGALFALVIFVQMKT"
     gene            complement(5959..6246)
                     /locus_tag="VFV99_01110"
     CDS             complement(5959..6246)
                     /locus_tag="VFV99_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057919.1"
                     /translation="MCLVVAGGRPSDASIRDPQTAQLAAAPNAVIAASLARRGGIDSH
                     AQRGPLLFVVPVATVDVDEPSRAPIDITATAPHSLVSSFVPARSARGPPRS"
     gene            complement(6316..7797)
                     /locus_tag="VFV99_01115"
     CDS             complement(6316..7797)
                     /locus_tag="VFV99_01115"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5057920.1"
                     /translation="MKPANILDIVRAIAGYHERDELLPALVRGLRAAVAADVYLVAIR
                     RGRTGDRFDAYAEPPLGEYSNLISDYVPPPQLPNHPQALLLRVADLSTARHKELWRRH
                     GIQCAVRIPIVIEGALVGSISLWSREAETFDTFDLDVATELGVAVAVVLDSCLAYEHL
                     AEQRDDADDANIYLRHALATAHADGGLIGHGGAFGAVKQRIEVVAPTDATVLLTGESG
                     TGKDMVARAIHASSSRRDRPLVTVNCAAIPASLAESELFGHEEGAFTGATTSRAGRFE
                     QANGGTLFLDEIAELSLDVQAKLLRVLQERELERIGGGEPIKLDVRIIAATNRDLQEL
                     IEVDRFREDLYFRLAVFPIHLPPLRSRLEDLPELVAFFVHQASDRLHVPPRRLTPEGL
                     RRLADHDWPGNVRELQHAVERAMIVSTGDSLSIDAMVPRRIAPISTGADSDPLRREYL
                     AALESTSWQIEGDSGAAAQLGVHPNTLRYRLKRMGITRPPRMR"
     gene            complement(7794..9248)
                     /locus_tag="VFV99_01120"
     CDS             complement(7794..9248)
                     /locus_tag="VFV99_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5057921.1"
                     /translation="MLRVFEAVSRHRDREHLFTAIAGVLRQVFEFDALIVLLAGPGPD
                     QMTPYFIQPRVAIPALQRSRSALDTVFRTGQPLYVRTRADVADRPGSVESMQRFGAQS
                     YVALPLDVRGEVIAVLLLQSNREHAFDDLDISFATEIAGAIALALDNCLAYERIVRSR
                     ALLESENQLLRAELSAARPPGELVAESPAMRDIVRLVNLVAPTDATVLITGETGTGKE
                     RLARLIHERSQRADRPLVAVNCAAIPTPLVESELFGHEAGAFTGALRRRRGRFELADG
                     GTLLLDEVGELAFDAQAKLLRVLQTQEIDRVGGQEAVRVNVRVIAATNRPLIEMVHAR
                     RFRGDLYYRLAVFPLDLPPLCDRHDDIPILAHQLIVESARRLGLAPPHLDEETIVELE
                     RYGWPGNVRELQNVIERAVILSRGGLLDVGALLEVAPEPIVDAGSPEANEMLAALEAS
                     RWVIEGDDGAASRLRMRPSTLRSRMRKYGIERRR"
     gene            9563..10534
                     /locus_tag="VFV99_01125"
     CDS             9563..10534
                     /locus_tag="VFV99_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057922.1"
                     /translation="MSAVLVVARGPGLPTGVLLGETDRKQSPELDRVVLAELLTNAHD
                     DKVVLALARLLFGDNQPIEHMVSRDRDGVIVGVVAVRRNGPSSTWGTGVLASTANSLA
                     GWLSTPTAAGLPPHALAEALEEPVIAHEGGVVIVANRAVAKLLACEPSDLIGAPITRV
                     TQRLSTSRTSCLVVGGVPRTALVFDRPPSRILTNVRATVESVLARHYPLVRQTACVAF
                     DRREDVTALVAATAADELVTLALLEMSAIFGTSMPENQLRISVYRKKPWAVIELVASG
                     KIAPAPDVEHVGSLVCAARTRTAGGQFMLDASQPNARTLRISLPVES"
     gene            10531..10905
                     /locus_tag="VFV99_01130"
     CDS             10531..10905
                     /locus_tag="VFV99_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057923.1"
                     /translation="MSAELQLVTDDIELVAVVGSLLSEVTTLRLVALADAGESDAETP
                     PIVLVDVRKNITPASIRRLRIRHRRALFVAIVAAQQGPRELYIEGAHAVVPADSRAIA
                     VCLSVLFQGPRLEPATRATAIR"
     gene            complement(10857..>11521)
                     /locus_tag="VFV99_01135"
     CDS             complement(10857..>11521)
                     /locus_tag="VFV99_01135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5057924.1"
                     /translation="RTRFIDDVVRAERARGIRYYVILGAGYDSRAHRLAELKEARVFE
                     VDRAETQAEKRRRIESVPGVRKDVRYVAVDFEKQDLATRLADGGWRADRRTVFIWEGV
                     TNYLDAKAVATVLDFVGRTKPESVIVFTYIHRGVIDGSVTFAGADKLVGNVQRLGEPW
                     RFGFVPGELAAYLAELGLTLEQDLGADDYRSRYFGANAKFDGYAFYRIAVARVAGSKR
                     GP"
BASE COUNT         2049 a         3808 c         3793 g         1871 t
ORIGIN      
        1 gaagcggtct ggtactgcga cgcgatgctc gccggtggca agaacgcgaa tggtgtcttc
       61 actgcgacga aggaccccgc gatcgctacg ctcgttcgcc aggtcaaggc agacatcggc
      121 gagttccgtc gcaagggcga cgctcgctac gcgctcttca agagtggcag caacactgca
      181 gccgtcggca gcacgctcga caccgagttc gacacgatct tcgagaactt cctcaagaac
      241 gccgacgatg cagcgctgct catcgatcgc tcgatcgatc gctcggtcga gggcctcgac
      301 gcgatggaga gcagtgcgcg cacgttcatg gtcgtcacga tcgtgctcgg cgtggcgatg
      361 gcgctcgcgc tcgggatgtt gatcgcgcgc agcatcagtc gcccgctccg ccaggccgtg
      421 gacgccgcga atcgcgtcgc gctcggtcat gtcgacgtga agctcgaggg gacgacgcgc
      481 gacgaaaccg gcatgttgct ccaggcgatg cgcgagatga tggacgcaaa ccgcgcgatg
      541 tcgatcgccg cccaggacat cgctgccggc aatctcgccg tgaaagtcgt gccgcgttcg
      601 gacaaggatg cactcggaca ggcgctgtcg accatggtta cgaatctcgc tgcgacgatc
      661 ggcgaggtcc gctcggcggc ggctgcacta tcgagtgcgg ccgagcaagt cgccgccagc
      721 tcgcaaagcc tgtcgcaagg cacgagcgag caagcgagcg cggtcgaaga gaccagctcg
      781 acactggagg aaatgaacgc atcgatcacg cagaacgccg agaacagccg ccgcaccgag
      841 ctgatgtcgg tcaaaggcgc ggtcgacgcc gaggagagcg gtaagaccgt cgaggaaacc
      901 tcgaaggcga tggagaccat cgcggacaag gtctcgatca tcgaagagat cgcgtaccag
      961 acgaacctgc tcgcactgaa cgcggcgatc gaagcagcgc gcgctggtga ccatggacgt
     1021 ggcttcggtg tcgtcgcgaa cgaggtgcgc aagctggccg agcgcagcca gggttcggcg
     1081 agagagatca cgcagctcgc gggtgcgagc gtgaaggtcg ccggccggtc gcaggcgctg
     1141 ctcgccgagc tcgtgccttc gatccgcaag acgaccgagg tcgttcagga ggtcgccgct
     1201 gcgtcgcagg agcaagcgtc aggcgtcaac caggtgagca aggcgatcgc gcagatggac
     1261 atggtcacgc agcgcaatgc cgccgcggcg gaggagctgt cgagcaccgc ggaagagctg
     1321 tcggcacagg ccgaagcgct ccagcagctg atgcagtact tcaagctgca gggcaccgag
     1381 caccagacga agacgcccgt gatcgaccgc cggctgtcga agccgacgct ggtgtacccg
     1441 aacggcaagc acgtcggcct caacggtgca catgccgcca tccccgaggg cgagttccgc
     1501 gcgttctgag ggaaccatgg agaccaagcg ctatctgacg ttctacgtcg gtgaccagga
     1561 atacggcatc gacatcctcg agtcgcgagc ggtcgtgcag tttccgacca tcacgcgact
     1621 gccgtggatg ccggcgtcga tccgcggcgt gatcaacatg cgcggcagca tcctgccggt
     1681 ggtcgatctc gcggtgctgt tcggctgcga ggcacgtccg accacgaact ggacgtgcgt
     1741 ggtcgttgtc gaaagcgcgg gcgcaccggg cgaacagagt cggctcggtc tcgtggtcga
     1801 tagcgtcagc caagtgatcg aggtgcgtgg cgaagacctc gaggccgtgc cagcgtttgg
     1861 aacgcgcatc ccgacgaagt tcctgcgcgg aattgtcact gctggcgcca cgttcgttca
     1921 cgtgctcgat ctggcccacg tcgtcgaaca cgtcgtgcat cagctgcagc gccgcgaagc
     1981 attgtccgca gtcgttgctg ccgaatcgag tgagggactg caatgagcgc tgctcctgct
     2041 ctcgctgcgc cggcgctgtc ggagcgcgag ttccagctgt tccagaagtt ggtccttcgc
     2101 gaagctggca tctttctcgg cgagcacaag cgcgcgctgg tcatcggccg gctatcgcgc
     2161 cgcttgcgcg agctgggcat gacgtcgttc ggcgtgtact acgaccacgt cactcgcagc
     2221 ggcgatgccg agctcgttca cctgctcgac gcgatctgca cgaacgagac gcacttcttc
     2281 cgcgagcccg atcacttctc gttcctcgag tcgcacgcgc tgcgcgaatt cgaagcgctc
     2341 gccgccgccg ggaagattcc gaagcggctc cgggtgtgga gcgctgcgtg ctcctcgggt
     2401 gaggagccgt attcgctcgc gatgacactg cgcgcgcggt ttcctcgcga gtcgggatgg
     2461 catctcgaga tactcgcgag cgatctatcg acgcggatcc tcgatcgcgc ccgcgcggcg
     2521 acctggccga tcgagaagac atcggagatt ccgtacgact acctgcgccg cttcatgcta
     2581 cgtggcaccg gttcgcagtc aggcaagatg aaggcggggg cggagatccg gagcctcgtc
     2641 gagttctcgc gcatcaacct gaacgacgac aactggcccg tcgcaggcaa gttcgatctg
     2701 atcttctgcc gcaacgtcct catctacttc gagcgcactg gcaaggcgaa agtcgcgcgc
     2761 cacctgctcg acagactgtc cccgcacggc tacttgttcc tcggtcacgc cgagacgctg
     2821 gccgggcttt ccgatcaggt gcgcagcgtc agcccgagcg tgtaccagct gaggcagcgc
     2881 gtcaccgagg attgttaatg ccgcagtctc ccgcgcgact gcacgtactc gtcgtcgacg
     2941 actccgcggt tgtccgcacc gcgctgcagg cgctgctcgc gaaggatcca ggcatcaccg
     3001 ttgcagtcgc atcggatccg atcatcgcga tgaccaagat caagatgtcg cgtcccgacg
     3061 tgatcgtcct cgatctgcaa atgcctcgca tggatggcct gacgttcctc gctcgcttga
     3121 tggcggagca gccgcttccg gtcgtcgtgt gctcgagcca ggcaggaccg agcaccgagg
     3181 ctgcactacg cgcgttgatg ctcggcgcag tcgacctgat tccaaagcca cagatcggcg
     3241 tgcgtgactt tctcgagcac tcggtcactc agttcatcga gaccattcgc ggcgcggcgc
     3301 acgcacgcgt gaaccgccgc gtcatgccag cggctcccgc cgccagcgtc gcagtccagc
     3361 caagggccgt gtcgcccgag taccgtgcca gcgatcgagt ggtggcgatc ggcgcatcga
     3421 ccggaggaac cgaggcgttg cgccagatcc tgtcggcgat gcccgcgaat ggtccggcga
     3481 tggtcatcgt ccagcacatg ccggccggtt tcaccgcagc gttcgcgaag cagttgagcg
     3541 agacctgcgc catcgaagtc aaagaggcgg ccgatggcga tctgctggtc aacggacggg
     3601 cgctcgtcgc gccaggcaat cgccacgtgc atctggtgcg cagcggcagc cgctacgccg
     3661 tgcgggtcac cgacggcgcg ccggtgtctc gccaccgccc gagtgtcgat gtgttgtttc
     3721 ggtccgtcgc cgcggtcgca ggacgcaacg cggttggcgt gatcatgact ggcatgggcc
     3781 acgacggagc cgagggcttg cgcgacatgc gcgaggcggg tgcgtacacg ttggctcagg
     3841 acgaggcgag ttgtgtcgtg ttcggaatgc cgagggaagc gatcgcgcgc ggcggcgtcg
     3901 atcgcgtggt gagtttgtcc cacttaccag cggccatcct gcagagcgtc cagccggctg
     3961 gccactgagt cacccgagga gaccatgagc tcgaacccga tcgatgtcga cgaccgcatg
     4021 gcggagcgtt gcgaatacct gacgttcttc gtgcgcggcg aggaatacgc agtcggtctg
     4081 cggcgcgtgc gcgaagtgat cgcgttcaac aagatcacgc gcattccgca catgccgcct
     4141 gcgctgcgtg gcgtcactgg ctttcgcggc gaggtggtgc ccgtgctgga tctcgcgatc
     4201 aagttccacg gcatcgagac ggctgtcgat ccgaagctct gcgccagcct catgctcgtc
     4261 gagaccgtga tcgacgggcg gccgcgcgtg ctcggcctca tcaccgaagc ggtgggtcag
     4321 gtgctcgcac tgaccgatgc cgacgtcgtg ccaccgccga gcttcggtgc accgatccga
     4381 accgagttcc tcgccggcag gggccgtgtg ggtacgaagt tcgcgctact cctcgacatc
     4441 gatcggctgc ttaccttcga cgagctgctg tccgcgcagc ttgccgagcc ccttgccgtc
     4501 accgacgctg ccgtctaatg tccggccgtg ggccgcgcat tcgagaaacg caaacacacg
     4561 atcttcgcgc gcatggatcg cgtcgcgaaa cagttcacga agatcaacaa agacatcgcg
     4621 atcgccgtca aggcgagtgg gccgaacccg gaaggtaatc ccgcgctgcg ccgcgcgctg
     4681 cagaacgcac gtgcgctcaa catgccgaag gacaaggtcg aagccgcgat caagaaggcg
     4741 agcggtcagg gcgcgacgaa ctacgagatc gtgctctacg aaggctacgc accgcacgga
     4801 atcgcattgc tcgtggagac cgcgaccgac aacgtcacgc gcacggtcgc gaacatccgc
     4861 caccacttca aggcgaacgg cggcaacatg ggcacgaccg gcagcgtcgc gttcatgttc
     4921 cagaagcagg gcgtgttccg gctcaatccc gagggcatcg accgcgacac cctcgagctc
     4981 gagctgatcg atcatggcct cgtggcgctc gaggacgggg agagcgacaa gggcgagaag
     5041 cagctggtcg tgcgctgcgc gttcaccgac ttcggcacga tgcagagcgc cctcgaggct
     5101 cgcaacatca cgccgatctc gtcgagctcc gattacgccg ccgccacgca gaccgagctc
     5161 cccgacgaca aagccgacga agtgatgaag ctcgtcgccg cgatggaaga ggacgacgac
     5221 gtgcagaacg tctacacgaa catggcgtag tgctgatcgc gctcaataag ccgtacctcg
     5281 tccactgcaa attcaccgat cagcaagggc ggccgacgct cgcagcgttc ggcctcccgc
     5341 cgcgcgtgta cgtcgctggt cgtctcgatc acgatagcga gggcctgctg ctgctcaccg
     5401 acgacggcaa gctgaaccag cggctgaccg atccgcgctg gaaagcgccg aagacgtact
     5461 gcgtgcaggt cgaaggcaca ccgacggaag aagcgctcgc gggcctgcgg gccggcgtcg
     5521 atctcaatgt cggccgtacg ctacctgcgg acgcgcgtgc gatcgacgag ccaccgtggc
     5581 tatggccacg aacgccgccg attcgcgtac gcaaaacggt gcccgatgcg tggatcgagc
     5641 tgacgatccg cgaaggacgc aatcgtcagg ttcgccgcat gaccgcggcg gtagggttgc
     5701 cgacgcttcg actgattcgc gtcgcgatcg gcccctactc gctcgacggc ctcgcgcccg
     5761 ggcaatggcg cgagcaggtg gtcacgtctt catctgcacg aagatgacga gcgcgaacag
     5821 cgctcccatc acagcaataa tgatcgtgag cgcgttacgt tgccagaacg tgaggttctg
     5881 gcgcggcacg ctacccgagt catcggattg catggctagc tcctttggta gtggttgcag
     5941 tgatcggtga acgggcgatc agctgcgcgg aggaccacgt gcggagcgag caggaacaaa
     6001 cgagctaacg agcgagtgcg gcgcggtggc cgtgatgtcg atcggcgcgc gcgacggctc
     6061 atcgacatca acggtcgcga ccggaacgac gaacagcagc ggtccgcgct gcgcgtgcga
     6121 atcgatgccg ccacggcgcg ccagcgaggc cgcgatgact gcattcggtg cggccgcgag
     6181 ctgcgcggtc tgcggatcgc ggatcgatgc atcgctcggt cgcccaccgg cgacgaccaa
     6241 gcacaacgcg aacgcgatgg aactgatgaa ccgcagacct actgcgtagc atcccggcgc
     6301 accgtgccgc aacgctcaac gcatccgcgg cggccgtgta atgcccatcc gcttgagccg
     6361 ataccgcagc gtgttcgggt gaacgccgag ctgcgctgcg gctccgctat caccttcgat
     6421 ctgccagctg gtcgactcga gagctgcgag gtactcgcgg cgcagtggat cactgtcggc
     6481 accggtgctg atgggagcga tgcgacgcgg caccatcgcg tcgatcgaca gcgaatcgcc
     6541 ggtgctgacg atcatcgcgc gctcgacggc gtgctggagc tcgcgcacgt tccccggcca
     6601 gtcgtgatcg gcgagccggc gaagcccctc gggcgtcagc cgccgcggtg gcacgtgaag
     6661 ccggtcggag gcctggtgca cgaagaacgc gacgagctcc ggcaggtctt cgaggcgcga
     6721 ccgcagcggc ggcagatgga tcgggaacac ggcgaggcgg aagtagaggt cttcgcgaaa
     6781 gcgatcgacc tcgatcagct cctggagatc gcgattcgtc gccgcgatga tgcggacatc
     6841 gagcttgatc ggctcgccgc caccaatgcg ctccagctcg cgctcctgca gcacacgcaa
     6901 cagctttgcc tgcacgtcga gcgacagctc cgcgatctcg tcgaggaaca gcgtgccgcc
     6961 attcgcctgc tcgaaccggc cggcccgcga cgtcgtggcg cccgtgaatg cgccctcttc
     7021 atgaccgaac agctcgctct cggcgaggct cgccggaatc gccgcgcagt tgaccgtgac
     7081 aaggggcctg tcgcggcgtg agctcgatgc gtgaatcgca cgcgccacca tgtccttgcc
     7141 cgtgccactc tcaccggtga gcagcaccgt cgcatcggtc ggcgcgacca cttcgattcg
     7201 ctgcttgacc gcgccgaacg cgccgccgtg gccgatcaag ccgccatcag cgtgtgccgt
     7261 cgcgagcgcg tggcgcaggt agatgtttgc atcgtcggca tcgtcgcgct gctccgcgag
     7321 gtgttcgtac gcgagacagc tgtcgagcac gacggcaacc gcgacgccga gctcggtcgc
     7381 gacatcgaga tcgaacgtat cgaacgtctc ggcctcgcgc gaccagaggg aaatgctccc
     7441 gacgagtgca ccctcgatga cgatcgggat gcgcaccgcg cactgaatgc cgtgccgccg
     7501 ccacagttct ttgtgcctcg cggtgctcaa gtcagcgacg cgcagcagga gcgcctgcgg
     7561 gtgattcgga agctgcggcg gagggacgta gtcggagatc agattcgagt attcgccaag
     7621 cgggggctcg gcgtaggcgt cgaaccgatc accggtgcgg ccccgacgaa ttgcgaccag
     7681 atagacgtcc gcagcaacgg cggcgcgcaa gccgcgcacg agcgccggta acagctcgtc
     7741 gcgttcgtga taaccggcga tcgcacgcac gatgtcgaga atgttggcgg gcttcatcgc
     7801 cggcgctcga ttccgtattt gcgcattcgc gatcgcaacg tgctcggccg catgcggagc
     7861 cggctcgccg caccgtcatc gccttcgatc acccaacgac tcgcctccag tgccgcgagc
     7921 atctcgttcg cctccggcga gccggcatcg acaatcggtt ccggcgcgac ctcgagcaat
     7981 gccccgacat cgagcaagcc accgcgcgac aggatcaccg cgcgctcgat cacgttctgg
     8041 agctcgcgta cgttgcccgg ccacccgtag cgctcgagct ccacgatcgt ctcctcgtcg
     8101 aggtgcggcg gcgccaagcc gagcctccgc gcgctctcga cgatcagctg gtgcgcgagg
     8161 attggaatgt cgtcatggcg gtcgcagagc ggcggcagat cgagcgggaa caccgcgaga
     8221 cggtagtaga ggtcaccgcg aaaccgccgc gcgtgcacca tctcgatcag cggccggttc
     8281 gtcgctgcga tcacgcgcac gttgacgcgc acagcttcct gtccgccaac gcgatcgatc
     8341 tcctgcgtct gcaatacgcg caacagcttc gcctgcgcat cgaacgcgag ctcgccgacc
     8401 tcgtcgagca gcagagtgcc gccatcggcc agctcgaagc ggccacgccg ccggcgcagc
     8461 gcaccggtga acgcaccagc ttcgtgaccg aacagctcgc tctcgacgag cggcgtcgga
     8521 atcgcggcac agttgaccgc gaccaacggg cgatcggcgc gctggctgcg ctcgtggatc
     8581 agacgcgcga gccgctcttt gccggtgccg gtctcgccgg tgatcaggac ggtcgcatcg
     8641 gtcggcgcca cgagattcac gaggcgcacg atgtcgcgca tcgcggggct ctcggcgacg
     8701 agctcgccag gtggccgcgc cgcggagagc tccgcgcgca gcagctggtt ctcggattcg
     8761 agcagggccc gcgatcgcac gatgcgctcg taggccaggc agttgtcgag cgcgagtgcg
     8821 atggcgccgg cgatctcggt cgcgaatgaa atgtcgaggt cgtcgaatgc gtgctcgcgg
     8881 ttcgactgca gcagcagcac cgcgatcacc tcgccgcgta cgtcgagcgg cagcgcgacg
     8941 tacgactgcg cgccaaatcg ctgcatcgac tcgaccgagc ccgggcggtc ggccacgtcc
     9001 gcgcgggttc tgacatacag cggctgcccg gtccgaaata cggtgtcgag cgccgaccgc
     9061 gaccgctgga gcgccgggat cgcgacgcgt ggctgaatga agtagggagt catctgatcc
     9121 ggccccggac cagccagcag cacgatcaga gcgtcgaact cgaatacctg ccggagtacg
     9181 cccgcgatcg ccgtgaacag gtgctcgcgg tcgcggtggc gggagacggc ctcgaagacg
     9241 cgcagcaccg tcctcgcttg ctcgtctgac cagttggacg gctccacaac gacctgccct
     9301 tacgtacagc ctagtccctc tccaccacct cgcacgccac gaggacgtcc gtggttcgtg
     9361 ggacgtcgtg ggcgcacgcc gactcctatc gaccagttag cgcgggcacg ccggttgcct
     9421 tgcaagacct gcgtcgatgc acgcagcaaa ggaagaagac tggatgtctg acgagcccac
     9481 ggtagtggag gaagaacgtg aggccactgc tagagagaag atcgcgcggg cgctggccga
     9541 cctgttgatg acgacccttt cggtgtcagc cgtcctcgta gtggcgcgcg gcccggggct
     9601 gccgaccggc gtgctgctcg gggagaccga tcgcaagcag agccccgagc tcgaccgcgt
     9661 cgtgctcgcc gagctcttga cgaatgcaca tgacgataag gtggttctcg cactcgcccg
     9721 gctgctgttc ggagacaacc agccaatcga gcacatggtc tcgcgcgacc gcgatggcgt
     9781 gatcgtcggc gtggttgcgg ttcgccgcaa cggtccgtct tcgacgtggg gaaccggcgt
     9841 gctcgcgagc accgcgaaca gcctggccgg ttggctatcg acgccgactg cagccggcct
     9901 gcccccgcac gcactcgccg aagcgctcga ggaaccggtc atcgcgcacg aaggtggcgt
     9961 cgtgattgtc gcgaaccgcg cggtggccaa gctgctcgcg tgcgagccaa gcgatctgat
    10021 cggtgcaccg atcacgcgcg tcacccaacg cctgtcgaca tcgcgaacca gctgcctggt
    10081 cgtcggcggt gttccacgca cggcgctcgt cttcgatcga ccgccgtcgc gcatcctgac
    10141 gaacgtgcgc gcgaccgtcg agagcgtgct cgcgcggcac tacccgctcg tgcgccagac
    10201 ggcgtgcgtt gcgttcgatc gccgcgaaga cgtcaccgcg ctcgtggcag cgaccgccgc
    10261 cgacgagctg gtgacactgg ccctgctcga gatgagcgcg atcttcggca cgtcgatgcc
    10321 ggagaatcag ctccgcatca gcgtctatcg caagaagccg tgggccgtga tcgagctcgt
    10381 cgcgagcggc aagatcgcgc cggcgcccga tgtcgagcac gtcggctcgc tcgtctgcgc
    10441 tgctcggact cgcaccgcag gtggccagtt catgctcgat gcatcacagc cgaacgcgcg
    10501 cacgctgcgc atttcccttc cggtggaatc atgagtgcag agcttcagct cgttaccgat
    10561 gacatcgagc tcgtcgccgt cgtcggctcg ctactctcag aggtcacgac gctgcgtctc
    10621 gtcgcgctag cagatgctgg cgaatctgac gccgagacgc cgcccatcgt gctcgtcgac
    10681 gttcgcaaga acatcacgcc cgcgtcgatt cgccggctgc ggatccggca tcggcgtgcg
    10741 ttgttcgtcg cgatcgtggc ggcccagcag ggtccgcgcg agctctacat cgagggcgca
    10801 cacgccgtgg tgccggccga cagtcgcgca attgcggtct gcctctcggt gttgtttcag
    10861 ggaccgcgtt tagaaccggc gactcgtgcc acggcgatcc gataaaacgc gtagccgtcg
    10921 aacttcgcat tcgcgccgaa gtagcgcgaa cgatagtcgt cagcaccaag atcctgctct
    10981 agcgttagcc cgagctcggc gaggtatgcg gctagctcgc ccggtacgaa cccgaaccgc
    11041 cacggctcgc caagacgctg cacgttgccc acaagcttgt cagccccggc aaacgtcacg
    11101 ctcccgtcga tgacgccgcg atggatgtac gtgaacacga tcacgctctc cggcttcgtc
    11161 ctgccgacaa aatcgagcac ggtcgcgacc gccttggcat cgaggtagtt cgtcacgccc
    11221 tcccagatga atacggtcct gcgatccgcg cgccagccac cgtcggcaag ccgtgttgcg
    11281 aggtcctgct tctcgaagtc gaccgcgacg tagcgcacgt ctttgcgcac gccgggcacg
    11341 ctctcgatgc gccgccgctt ctcggcttgg gtctccgcgc gatcgacctc gaagacgcgc
    11401 gcttctttga gctcggccag gcgatgcgcc cgcgagtcgt aacccgcgcc aagaatcacg
    11461 tagtagcgaa tgccgcgagc acgttcggcg cgcacgacgt cgtcgatgaa tcgcgtgcga
    11521 c
//
LOCUS       DASUFX010000027         6450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1394259, whole genome shotgun sequence.
ACCESSION   DASUFX010000027 DASUFX010000000
VERSION     DASUFX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394259"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFV99_01140"
     CDS             complement(<1..525)
                     /locus_tag="VFV99_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5057925.1"
                     /translation="MPSRAVLVLALTVGCHRTPPPVPIAPLPPNAYSHYLAGKLALYR
                     DDAATAADELRIAAQAAPDQPMITVELARALAKAKREDAARDVLVLARKKWPEHAQVW
                     LASGEVLEDGAKTRPEAMAAYRRAIKLEPTEERAYIGLARTQAAMGDAAGSEKTLRAL
                     VKKLPTSVEGHYRLA"
     gene            753..3272
                     /locus_tag="VFV99_01145"
     CDS             753..3272
                     /locus_tag="VFV99_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5057926.1"
                     /translation="MPAVGTSATTPTAQEPATVSTYNKPPQHVLDVLHAPSPPSPYIS
                     PKRDRILLVSWVDYPSITQVAEPFLRLAGVRVEPRTRRKHDTPGGYGVAPCAHYFAMV
                     DIASSKESPLTLPAGGCVDGIAWAGDGKHFAFRNTAATAVELWIGDAATGVTKKVANA
                     ALNPMLGSSLQWLPDNKTLLVKLVPANAGQPPAGGTAAIGPSIQETDGKGGEFSTYEA
                     RDTLQNKHDEDLFDYYATSQLAFVDTSSGAIAPLGKPAVFSDLDVAPDGDHILVTRVV
                     KPYAYTVTYGRFPHEIDVWDRAARATTIAKLPLADHVPVSGVPTGPRDHGWRSTEPAT
                     LVWAEALDGGDWNAKVPARDKVMMQSAPFTSPPVEVTKTEQRFGGYDWSEQKSIALLH
                     EYDENKHWQRTFITNVDDPKAKPVLLWDMSYDERYKDPGNPVYKQMPNGQWAVRVDGG
                     KTIYLSGTGASTEGDRPFLDRLDLSTKKTERLFRSEKSAYESFLAFSDDKTTTSFLTW
                     HQTPSDPPNAFVRTLGDAVKGAPVTEAGFASASRAVTHIPDPTPAVRAIKKKLIKYKR
                     KDGTDLSFTLYLPPDYKEGQKYPAILYAYPLDYASAKEAGQVSGSEQYFTKIEDYRLL
                     LLSGYAIIDNAAFPIVGDPKKAYDTYLEQLVDDARAAVDKAVSLGMIDRNRIGVTGHS
                     HGALMTVNLLAHTDFFRAGVATSGSYNKTLTPFGFQNERRSVWKAPDIYRKVSPFFFA
                     DKIKHPLLLMHGAEDANPGTTPLQAVKLYEAVRGNGGVARLVMLPHEPHWYSAMESNE
                     QLAYEELRWFDTYVKNAPDKGADKKPAKTAGGSKQPDKAAQ"
     gene            complement(3537..5090)
                     /locus_tag="VFV99_01150"
     CDS             complement(3537..5090)
                     /locus_tag="VFV99_01150"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5057927.1"
                     /translation="MKDPIARLEEMEAKAALAGGQARIDKMHEAGKLTARERISALLD
                     EGSFVELDKFVTHRCADFGMQEQKILGDGVVTGHGTVDGRPVCVFAQDFTVFGGSLSG
                     AYAQKICKVMDLATKIGCPVIGLNDSGGARIQEGVESLAGYADIFLRNVLTSGVVPQL
                     SAIMGPCAGGAVYSPAITDFILMVDRTSYMFITGPEVIKTVTHEEVTKEQLGGAATHA
                     SRSGVSHFTESDELACINRIRELLSFMPGNNAEDPPIKETQDPPDRAEESLDTVVPQE
                     SNKPYDIKKIITAVVDDGKFVEVHKDYAKNIVCGFARFEGRPAGIVANQPNHLAGCLD
                     IDASIKAARFVRFCDCFNIPLVTFVDVPGFLPGTNQEYGGIIKHGAKLLFAFAEATVP
                     KITVITRKAYGGAYDVMSSKHIRADVNVSYPAAEIAVMGPEGAVNIIYRSELEKIKDE
                     TARAEARAKFTEHYRDTFANPYKAASLGYIDEIIRPRDTRWTIIRSLRTLKNKRQANL
                     PRKHGNIPL"
     gene            5113..5670
                     /locus_tag="VFV99_01155"
     CDS             5113..5670
                     /locus_tag="VFV99_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057928.1"
                     /translation="MTNVKGSALSSRVLWVQLEHGDAGYKRLLNQISPELRQTIEMGV
                     NKAKWYPFDQFVELNLVLDRLFGNGDLGLIKELGRFGADANLKTIYRLFYKVGTVHWI
                     LGRAVRLWTAHYDSGFMEVATRGPKAAVLRMRGFQTPHQAHCLSVQGWCERSIELSGG
                     RDVVVKESKCRTRGDDLCQLEAIWS"
     gene            complement(5671..>6450)
                     /locus_tag="VFV99_01160"
     CDS             complement(5671..>6450)
                     /locus_tag="VFV99_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057929.1"
                     /translation="DILGREGKRELALRTLDGLVDLDPDRANLHERMINAYERTGRLA
                     QACGHRIALVSLSVKEAVKRAGAAARCLRSLGKTADADLVMASLVDDKARTEAEKAAT
                     VAAVEPRVAGDLVINGKWDGASDLDISLVAPDGSRVSWMGGRTDITVADSTAHDREQL
                     SVKRLRKGNYLIEINRGEPAKSTVRGTLDVSVLGQKKSMPFELTGDRMVVGKIAVTLS
                     SHLEPVNPGDARWLNQGWQQQQWQQNNRRRGGRPGLIEGDL"
BASE COUNT         1122 a         2114 c         2097 g         1117 t
ORIGIN      
        1 cgcgagacga taatggccct cgacggaggt cggtagcttc ttgaccagcg cgcgcaacgt
       61 cttctccgag cctgcggcgt ctcccatcgc ggcctgcgtg cgggcgaggc cgatgtacgc
      121 gcgttcctcg gtcggctcga gcttgatcgc gcggcggtac gcggccatcg cttcggggcg
      181 tgtcttcgcg ccgtcttcga gcacctcgcc ggacgcgagc cagacctgcg cgtgctcggg
      241 ccacttcttg cgcgcgagca cgagcacatc gcgcgcggca tcttcacgct tggccttcgc
      301 gagcgcacgc gcgagctcga cggtgatcat cggctggtcg ggggcggcct gcgctgcgat
      361 gcgaagctcg tcggcggcgg tcgccgcgtc atcgcgatag agcgcgagct tgccggcgag
      421 ataatgcgag tacgcattcg gcggtagcgg cgcgatcggc accggcggtg gcgtgcgatg
      481 gcacccgacg gtgagcgcga gaacgaggac agcgcgtgac ggcaacgacg catcgtatac
      541 cgcgtgtcgc acggtcgcac gtgtcgcagc cgccgagtca gcaacagcgc ctggctcgac
      601 gctgctggct acgctagtgt cgcgcgatgc ctcgcacgat ctcgcgcgaa ttcgttctcg
      661 ccgtcgcgat agcggcgtgc tcgcacacga cgcccgctcc gaattctccg cccgcagcag
      721 cgcccggtgg ggcgggccct accactgcag ccgtgcctgc agtcggtacg tccgcgacca
      781 cgcccacggc gcaggagccg gccacggtct cgacgtacaa caagccgccg cagcacgtac
      841 tcgacgtcct gcacgcgccg tcgccgccgt cgccgtacat cagcccgaag cgcgatcgca
      901 tcttgctcgt gtcgtgggtc gactatccgt cgatcacgca ggtcgcggag ccgtttctgc
      961 ggctcgcggg tgtgcgcgtg gagccgcgta cgcgtcgcaa gcacgacacg cccggtggct
     1021 acggcgttgc gccgtgcgcg cattacttcg cgatggtcga catcgcgtcg tcgaaggaat
     1081 cgccgctcac cttgcctgcc ggcggttgcg tcgacggcat cgcgtgggcc ggcgacggca
     1141 agcacttcgc gtttcgcaac actgcagcga ctgcggtcga gctgtggatc ggcgacgcgg
     1201 cgaccggcgt cacgaagaag gtcgcgaacg ccgcgctcaa cccgatgctc ggctcgtcgc
     1261 tgcaatggct gcccgacaac aagacgttgc tcgtgaagct cgtgccagca aacgcgggcc
     1321 agccgccggc tggtggcacc gcggcgatcg gacccagcat tcaggagacc gacggcaagg
     1381 gcggcgagtt cagcacgtac gaagcgcgcg acacgctgca aaacaagcac gacgaagatc
     1441 tgttcgatta ctacgcgacg agccagctcg cgttcgtcga tacgtcgagc ggggcgatcg
     1501 caccactcgg taagccggca gtgttctcgg atctcgacgt cgcgcccgac ggtgatcaca
     1561 tcctcgtcac gcgcgtcgtc aagccgtacg cgtacaccgt cacgtacggg cgcttcccgc
     1621 acgagatcga tgtctgggat cgcgcggcgc gcgcgaccac gatcgcgaag ctgccgctcg
     1681 ccgatcacgt cccggtcagc ggcgtaccga ccgggccgcg cgatcacggc tggcgctcga
     1741 ccgagccggc gacgctcgtg tgggccgaag cgctcgatgg cggcgactgg aacgcaaagg
     1801 ttccggcgcg cgacaaggtg atgatgcagt cggcgccgtt cacgtcacct ccggtcgagg
     1861 tgacgaaaac cgagcagcgc ttcggcggct atgactggag cgagcagaag tcgatcgcgc
     1921 ttctccacga gtacgacgag aacaagcact ggcagcgcac gttcatcacg aacgtcgacg
     1981 atccgaaggc caagcccgtc ctgctctggg acatgtccta cgacgagcgc tacaaggatc
     2041 cgggcaaccc ggtctacaag cagatgccga acgggcagtg ggcggtgcgc gtcgacggcg
     2101 gcaagacgat ctatctgtct ggaaccggcg cgtcgacaga aggcgatcgc ccgttcctcg
     2161 atcgtctcga tctgtcgacg aagaagaccg agcgtctgtt ccgcagcgag aagtctgcgt
     2221 acgagagctt cctcgcgttt tccgacgaca agacgacgac ctcgttcttg acgtggcacc
     2281 agacgccgag cgatccgccg aacgcattcg tccgcacgct tggtgatgcg gtcaaaggcg
     2341 cgccggtgac cgaggccggc ttcgcgtcgg cgtcgcgggc ggtcacgcac attcccgatc
     2401 cgacgccggc tgtccgcgcg atcaagaaga agctcatcaa gtacaagcgc aaggacggca
     2461 ccgacctgtc gttcacgctc tatctgccgc ccgactacaa ggagggccag aagtaccccg
     2521 cgatcctcta cgcgtaccca ctcgactacg cgagcgcgaa ggaagcaggc caggtcagtg
     2581 gctccgagca gtacttcacg aagatcgagg actaccggct gctgctgctc tccggctacg
     2641 cgatcatcga caacgccgcg ttcccgatcg tcggcgatcc caagaaggct tatgacacct
     2701 acctcgagca gctcgtcgac gatgcgcgcg cggccgtcga caaggccgtc tcgctcggca
     2761 tgatcgatcg caatcgcatc ggcgtcaccg gtcacagcca cggcgcgctc atgaccgtga
     2821 atctgctcgc gcacaccgac ttcttccgcg ccggcgtcgc gacgagcggc tcgtataaca
     2881 aaacgctcac gccgtttggc ttccagaacg agcgccgcag cgtgtggaag gcgcccgaca
     2941 tctaccgcaa ggtgtcgccg ttcttcttcg cggacaagat caagcatccg ctgctcctca
     3001 tgcacggtgc cgaggacgcg aatccgggca cgacgccgct gcaggccgtg aagctctacg
     3061 aggccgtgcg cgggaacggc ggcgttgcgc gcctcgtcat gctgccgcac gagccgcact
     3121 ggtattcggc gatggagtcg aacgagcagc tcgcctacga ggagctgcgc tggttcgaca
     3181 cctacgtcaa gaacgcgccg gacaagggtg cggacaagaa gcccgcgaag accgcaggcg
     3241 gctcgaagca gcccgacaaa gccgcgcagt agcggcgcca gaccggcttc gcgtcgaaga
     3301 gactgcgtca gcgcctgagg cgccgcagat gaaacgccga gatgccaagc cggccaagaa
     3361 cgccaagcac gtctcttgat cgcgttctgc tcgcgcggtc gttcgtgcag agcgcgcgtt
     3421 taggccagtt ctcaggtggc ggccttggct ctgtttcgcg tcttggcgtt gatctccggg
     3481 gcggcgaggc cgccttacgc cacgatcgtc cggatctgaa atcggggcta gcgacgctac
     3541 agcggaatgt tgccgtgctt gcgcggcagg ttcgcttgcc gcttgttctt gagcgtgcgg
     3601 agcgaacgaa tgatcgtcca ccgcgtgtcg cgcgggcgga tgatctcgtc gatgtagccg
     3661 agcgacgccg ccttgtacgg gttcgcgaac gtgtcgcggt agtgctcggt gaacttggcg
     3721 cgcgcttcgg cacgggcggt ctcgtctttg atcttctcga gctcgctgcg gtagatgatg
     3781 ttgaccgcgc cctccgggcc catcacggcg atctcggccg ccggatacga cacgttgacg
     3841 tcggcgcgaa tgtgcttgga cgacatgacg tcgtaggcac cgccatacgc cttgcgcgtg
     3901 atcacggtga tcttcggcac cgtcgcttct gcgaatgcga acagcagctt cgcaccgtgc
     3961 ttgatgatgc cgccgtactc ctggttcgtc cccggcaaga agcctggcac gtcgacaaac
     4021 gtgacaagcg gaatgttgaa gcaatcgcag aaccgcacga agcgcgcggc cttgatcgat
     4081 gcgtcgatat cgaggcagcc cgcgaggtgg ttcggctggt tcgcgacgat gccagcgggc
     4141 cggccttcga agcgcgcgaa gccgcacacg atgttcttgg cgtagtcctt gtggacttcg
     4201 acgaacttcc cgtcgtcgac gaccgccgtg atgatcttct tgatgtcgta cggcttgttc
     4261 gactcctgcg gcacgacggt gtcgagcgac tcctcggcgc gatccggcgg atcctgcgtc
     4321 tccttgatcg gcggatcctc ggcgttgttg ccgggcatga acgacagcag ctcgcgaatg
     4381 cgattgatgc acgcgagctc gtcggactcg gtgaagtgag acacgcccga ccgcgacgcg
     4441 tgggttgcgg cgccgccgag ctgttccttc gtcacctctt cgtgggtcac ggttttgatc
     4501 acctcgggac cggtgatgaa catgtacgac gtgcgatcga ccatcaggat gaagtcggtg
     4561 atcgcgggcg agtacaccgc gccgcctgca catgggccca tgatcgcgga gagctgcggc
     4621 acgacgccgc tcgtcagcac gttgcgcaga aagatgtcgg cgtagccggc aagcgactcg
     4681 acgccttctt gaatgcgcgc gccaccggag tcgttgagcc cgatcaccgg gcaaccgatc
     4741 ttcgtggcga ggtccatcac cttgcagatt ttttgtgcgt acgcccccga aagcgatccg
     4801 ccgaacacgg tgaagtcctg cgcaaacacg cacaccggtc gaccgtcgac ggtgccatga
     4861 cccgtcacga caccatcgcc tagaattttc tgttcctgca tgccgaagtc ggcgcagcga
     4921 tgggtgacga atttgtcgag ctcgacaaaa ctaccttcgt ccaacagcgc tgaaatgcgc
     4981 tcgcgagcgg tgagcttgcc ggcctcgtgc atcttgtcga tgcgggcttg gccacccgcg
     5041 agagcggcct tggcttccat ctcctcgaga cgggcaatcg gatctttcat acagggcgcg
     5101 tatagtagag gcgtgacgaa cgtaaaggga agcgcgctgt cgtcacgcgt cctctgggtg
     5161 cagttagagc acggggatgc cggctacaag cgcttgttga accagatctc gccggagctc
     5221 cgccagacaa ttgaaatggg cgtcaacaag gcgaagtggt acccgttcga ccaattcgtc
     5281 gagctgaacc tcgtactcga tcgcctgttc gggaacgggg atctgggcct catcaaggag
     5341 ctcggccgct tcggcgccga cgcaaacctc aagacgatct accgcctgtt ctacaaggtc
     5401 ggcaccgtgc actggatcct cggccgcgcg gtgcggctgt ggaccgcgca ctacgactcg
     5461 ggattcatgg aagtcgcaac ccgcggtccg aaggcagcgg tccttcggat gcgcgggttt
     5521 caaacgccgc accaggcgca ctgtctgtcg gtgcagggct ggtgcgaacg gtcgatcgag
     5581 ctgtcgggtg gcagggacgt ggtcgtcaag gaatcgaagt gccgcacgcg cggcgatgac
     5641 ctgtgtcagc tcgaagccat ctggtcgtga ctacagatca ccctcgatca gaccggggcg
     5701 accgccgcgg cggcgattgt tctgctgcca ctgctgctgc tgccagcctt ggttcagcca
     5761 gcgcgcgtcg cccggattca ccggctcgag atgcgagctg agcgtcaccg cgatcttgcc
     5821 gaccaccatg cgatcgccgg tcagctcgaa cggcatcgac ttcttctggc cgagcacgct
     5881 gacgtcgagc gtgccgcgca ccgtcgactt cgccggctcg ccgcggttga tctcgatcaa
     5941 gtagttgccc ttgcgcaggc gcttgaccga gagctgctcg cgatcgtgcg ccgtcgagtc
     6001 ggccaccgtg atgtcggtgc ggccgcccat ccacgacacg cggctgccgt cgggcgccac
     6061 gagcgagatg tcgagatcgc ttgcgccatc ccatttgccg ttgatcacga gatcaccggc
     6121 gacacgcggc tcgaccgccg cgacggtcgc ggccttctcg gcctcggtgc gcgccttgtc
     6181 gtcgacgagc gacgccatca cgagatccgc atcggcggtc ttgccgagcg aacgcagaca
     6241 gcgcgccgct gcacccgcac gcttgaccgc ttccttcacg gacaggctga cgagtgcaat
     6301 gcgatggcca cacgcttgcg cgaggcgacc ggtgcgctcg tatgcgttga tcatccgctc
     6361 gtgaaggttc gcgcgatctg gatcgagatc gacgagacca tcgagcgtac gcagcgcaag
     6421 ctcgcgcttg ccttcacggc cgaggatgtc
//
LOCUS       DASUFX010000028         4846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_144240,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000028 DASUFX010000000
VERSION     DASUFX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144240"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1594)
                     /locus_tag="VFV99_01165"
     CDS             complement(38..1594)
                     /locus_tag="VFV99_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057930.1"
                     /translation="MTQTATHRPLAEPFSTGASWLMRALAVSFVFHAVVAVAWWLMAK
                     PHDDSEVQLVDIEVAPPPPKAEALPAEVAKQAEQVAAAQGAQEPETPSEDFAVIDAGI
                     DAPVDAPGKRKKRSDAAADEMMAETADGGVGDGGEGDGGGTQVALGEFNGAGSGSQDV
                     QGVPGATDTAQGSGAPGMDNQPAVEGAPTSAGTAANLLAYFPPGHQITVLIRFDRLRK
                     TEWAQPAAKLFTPMPDYRGLFGDRDVAIVDQLDTLVISSPRPRDATATTLVGHTQMTR
                     AQMRDFLANPDTPIAWSTTKGGMLGKRSGKLFPNDKRVLLSPWRGWFVLAQPTDVTGL
                     TAASGGNLDAIEAKATLPPWLAAIRTIEDETNEVAKPAKPGEPAAKPDPKRGPALVLT
                     LKGPSKRYNLPDVGLGVTSAPSPGRVSLAMELVKQGWLVRGNIVFATEADAIEFVATA
                     QKAQTTVTDSRILSGLLKKQHALNAITGLSLARAGARVSYATSVSIADARALLAVAAQ
                     TLDAYFANPP"
     gene            1656..2054
                     /locus_tag="VFV99_01170"
     CDS             1656..2054
                     /locus_tag="VFV99_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057931.1"
                     /translation="MKLTKDQVAAVVTEASTKMSDPNYSAVMVGGFVQSQMPVSNFIQ
                     AHERELGGAEAIVSVIFHCALVVQMFQRAGGKIRTLSYEDLDAAARGEPLIRLQKAQL
                     PLHEFIHANVENAEAQNLIAMIALAIDGAA"
     gene            2078..2596
                     /locus_tag="VFV99_01175"
     CDS             2078..2596
                     /locus_tag="VFV99_01175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5057932.1"
                     /translation="MANPTATFDTSLGSFTAEIYLDKMPITANNFIKLAKQGFYDGLH
                     FHRVIKDFMIQFGCEYSKDPNSPKCGTGNSPFGNVQDEHPPAHKLSNEPGTLSMANTG
                     RPNSGGAQFFINTVHNAYLDWFTPGQSKHPVFGKITSGMDVIKKIETTPTAAGDRPKT
                     PVKMNKVTIQGM"
     gene            2673..>4846
                     /locus_tag="VFV99_01180"
     CDS             2673..>4846
                     /locus_tag="VFV99_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5057933.1"
                     /translation="MRAFVAIAVVASVAVADAAPRVVRGVVTVHGSTKPIPGATVLTE
                     RGELAVSDLDGYFSIEVTPADRELTITAPGYATRTIRVVAGDALLRIELSAAKGSEVI
                     EITGKAPEQTKPLSYQLTADEIRFLPGAANDVLRAAQVLPGVSRIPYSFGGLVLRGTS
                     PRDSAVFLDGIEVPIAFHFGGITSFYPGTMLDSLTVLNGGFDASHGHAQGGLVTLTTR
                     EPRTDRWRVGGTIGLLDSSVWAEGPLYTGGIIMGIRRSYLDTILDPFVEEDVPLPSYW
                     DAQIKSSFGDPRKRGRITPMIFTSIDRVASEDITATSLFVRAAAPYLRSKGPLTLRVV
                     PWVGWTQLSLTEEPRSESDQRARFRRPMFPAGVRGEVLRDYTWGHLRGGVDVDSGYLS
                     QFDVDVMGSSGKKFGAELYWTDLALWGETRIKLDGERFSVKPGLRVDAYGLSEELVVD
                     PRLNIFQQLTDKISLRQAIGRFHQPPIPADVDPFNGNPKLDGSYVDQASFGLDAEIYP
                     GVITSVTSFAGFGNQIGVEVPHGNDTSIEPNTGGLGPTFQLLLEKQLGFSSYRENLGR
                     ARLFGVESLLKGNIDRWFFLLAYTLSWSQRTDNPATGQGWRPFELDQRHNLNIAASYR
                     LAKWRFGARLQFVTGNPYSPTPAGEEIPMPMPFAGRLPAFFHLDVRVDRSWHRCWGDV
                     NLFFDIQNVTNRTNVEGREYGYTDEHPLGADEDIPGLPIVPF"
BASE COUNT          812 a         1640 c         1526 g          868 t
ORIGIN      
        1 gcatcgcgat gacccgcatc ctcatagcgt gaggcaatca gggtgggttt gcaaagtagg
       61 catcgagcgt ctgcgcggcc accgcaagca gtgcgcgggc gtcggcaatc gagaccgacg
      121 ttgcgtagga cacccgagcc ccggcgcgcg ccagcgacaa tccggtgatc gcgttgaggg
      181 cgtgctgctt cttgagcaac cccgagagga tgcggctgtc ggtgaccgtg gtctgcgcct
      241 tctgggcggt cgccacgaac tcgatcgcgt cggcctcggt ggcgaacacg atgttgccgc
      301 gcaccagcca gccctgcttg accagctcca tcgcgagcga cacccgcccg ggcgatggcg
      361 ccgaggtcac cccgagtccg acgtcgggaa ggttgtagcg cttgctcggt cccttgagcg
      421 tgagcacgag cgccggtccg cgcttcgggt cgggcttggc agcgggctcg cccggttttg
      481 ccggcttcgc cacctcgttg gtctcgtctt cgatcgtccg gatcgccgcg agccatggcg
      541 gcagcgtcgc cttcgcttcg atcgcatcga ggttgccgcc gctcgccgcc gtcaacccgg
      601 tcacgtcggt tggctgtgcg agcacgaacc agccgcgcca tggcgacagc agcacgcgct
      661 tgtcattcgg aaacagcttg cccgatcgtt tgccgagcat gccgcccttc gtcgtcgacc
      721 acgcgatcgg cgtgtcgggg ttcgcgagga agtcgcgcat ctgcgcgcgc gtcatctgcg
      781 tgtgaccgac gagcgtcgtc gctgttgcat cgcgcggacg cggcgagctg atcacgagcg
      841 tgtcgagctg atcgacgatc gctacgtcgc gatcgccgaa caagccacga tagtccggca
      901 tcggcgtgaa cagcttcgcc gccggctgcg cccactcggt cttgcgcagc cgatcgaagc
      961 ggatgagcac cgtgatctga tgaccgggcg gaaagtacgc gagcaagttg gccgcggtac
     1021 ccgcactcgt cggcgcgccc tcgaccgccg gctggttatc catgcccggc gcacccgagc
     1081 cttgcgccgt gtccgtcgcc cccggcacac cctgcacatc ctgcgacccg cttcccgcgc
     1141 cgttgaattc gccgagcgcg acctgcgttc cgccgccatc accttcgccg ccatcgccga
     1201 caccaccatc tgccgtctcc gccatcatct cgtccgccgc cgcgtcggac cgcttcttcc
     1261 tcttgccagg cgcatcgacc ggcgcatcga tgccggcatc gatcaccgcg aagtcctcgc
     1321 tcggcgtctc cggttcctgc gcaccttgcg cggccgcgac ctgttcggcc tgcttcgcaa
     1381 cttctgcggg gagcgcctcg gccttcggcg gcggcggcgc gacctcgatg tccaccagct
     1441 gcacctcact gtcgtcgtgc ggtttcgcca tgagccacca cgccacagcg acgacggcgt
     1501 ggaatacgaa cgacaccgcg agcgcgcgca tgagccacga cgcaccggtc gagaacggct
     1561 ctgctagcgg tctatgcgtc gcggtctggg tcatttctcg acggatgccc tcttgcgcag
     1621 tagcatccca ccatggctga gtccaggcag ggcacatgaa gctcaccaag gaccaagtag
     1681 cggcggttgt tacggaagcg tcgaccaaga tgagcgaccc gaactattcg gccgtcatgg
     1741 tcgggggctt cgtgcagtcg caaatgccgg tgtcgaactt catccaggcg cacgagcgcg
     1801 agctcggcgg cgcggaagcc atcgtcagcg tcatctttca ttgcgcgctc gtcgttcaga
     1861 tgtttcagcg cgccggcggc aagatcagga cactttcgta cgaagacctc gatgcagccg
     1921 cgcgcggtga gccgttgatc cgtctgcaaa aggcgcaact cccgctgcac gagttcattc
     1981 atgcgaacgt agaaaacgcc gaggcgcaga atctgatcgc gatgatcgcg ctcgcgatcg
     2041 acggcgccgc ctagcgaaac gtgttaggcc tgcggtcatg gcgaatccga ccgcaacttt
     2101 cgacacgtca ctcggtagtt tcaccgcgga gatctatctc gacaagatgc cgatcaccgc
     2161 gaacaacttc atcaagctcg cgaagcaagg cttctacgac ggccttcact tccaccgcgt
     2221 gatcaaggat ttcatgatcc agttcgggtg cgagtactcg aaggatccga acagcccgaa
     2281 gtgcggcacc ggtaactcgc cgttcggtaa cgttcaggac gagcacccgc cggcgcacaa
     2341 gctgtcgaac gagccgggca cgctctcgat ggcgaacacg ggccggccga actcgggcgg
     2401 cgcgcagttc ttcatcaata ccgtgcacaa cgcatatctc gattggttca cgcccggtca
     2461 gagcaagcac ccggtgtttg gcaagatcac ctcgggtatg gatgtcatca agaagatcga
     2521 gaccacgccg accgccgccg gcgaccgccc gaagacgccg gtcaagatga acaaggtcac
     2581 gatccagggc atgtaaatcc cggcgcgggt ggcacgagtt gccaccgccc ggcgcgagac
     2641 accatcgcct ggcatgtcat gctcgctcat tggtgcgagc gttcgtcgcc atcgcggtcg
     2701 tcgccagcgt agcggttgcg gacgctgcgc ctcgcgtcgt gcgcggagtc gtcaccgtac
     2761 acggctcgac gaagccgatc ccaggcgcga ccgtgctcac cgagcgcggc gagctcgcgg
     2821 tctctgatct cgacggttac ttctcgatcg aggtcacgcc tgccgatcgc gagctcacca
     2881 tcaccgcgcc cggctacgcg acccgcacga ttcgggtcgt cgcgggcgac gcgctgttgc
     2941 gtatcgagct gtctgccgcg aagggcagcg aggtcatcga gatcacgggc aaggcacccg
     3001 aacagacgaa gccgctgtcg taccagctca ccgccgacga gattcgcttc ctgcccggcg
     3061 ccgccaacga cgtgctgcgc gccgcacagg tgctgcccgg cgtgtcgcgc attccgtact
     3121 cgttcggtgg cctcgtactg cgcggcacgt caccgcgcga tagcgccgtg tttctcgacg
     3181 gcatcgaggt gccgatcgcg tttcacttcg gcggcatcac gtcgttctac ccgggcacga
     3241 tgctcgattc actcacggtc ttgaacggcg gcttcgatgc gtcgcacggc cacgcgcagg
     3301 gcggccttgt cacgctgacg acgcgcgagc cgcgcacgga tcgctggcgc gtcggcggca
     3361 cgatcggcct gctcgactcg agcgtgtggg cggaagggcc gctctacacc ggcggcatca
     3421 tcatgggtat ccgccggtcg tatctcgaca cgatcctcga tccgttcgtc gaggaagacg
     3481 tgccgctgcc gagctactgg gacgcgcaga tcaagtcgtc attcggcgat ccgcgcaagc
     3541 gcgggcgcat cacaccgatg atcttcacgt cgatcgatcg cgtcgcgagc gaggacatca
     3601 cggcgacgtc gttgttcgtg cgtgcggcgg cgccgtatct gcggtcgaag ggaccgctca
     3661 cgctgcgcgt cgtgccatgg gttgggtgga cgcagctgtc tctcaccgag gagccgcgca
     3721 gcgagagcga tcaacgcgcc cggttccggc gcccgatgtt cccagctggc gtacgcggcg
     3781 aggttctgcg cgactatacc tggggccacc tgcgcggcgg cgtcgacgtc gatagcggat
     3841 acctctctca gttcgacgtc gacgtcatgg gatcgagcgg caagaagttc ggcgccgagc
     3901 tgtactggac cgaccttgca ctgtggggcg agacgcggat caagctcgac ggcgaacgct
     3961 tctcggtgaa accaggtctg cgcgtcgatg cgtacggtct atcggaggag ctcgttgtcg
     4021 acccgcgcct caacatcttt cagcagctga ccgacaagat ttcgctccgt caggcgatcg
     4081 ggcgatttca ccagccgccg attcccgccg acgtcgatcc gttcaacggc aatccgaagc
     4141 tcgatggctc gtacgtcgat caagcgtcgt tcggactcga cgcggagatc tacccgggcg
     4201 tcatcacgtc ggtcacatcg ttcgccggct ttggcaatca gatcggcgtc gaagtgccac
     4261 acggcaacga cacgtcgatc gagccgaaca ccggcggcct ggggcccacg ttccagctct
     4321 tgctcgagaa gcagctcggc ttctcgtcgt accgcgagaa cctcggtcgt gcgcgcctct
     4381 ttggtgtcga gagtctgctc aagggcaaca tcgatcgctg gttcttcttg ctcgcgtaca
     4441 cgctgtcgtg gtcgcagcgc accgacaatc cggcgaccgg gcagggctgg cgtccgttcg
     4501 agctcgacca gcgtcacaac ctcaacatcg cagcgtcgta ccggctcgcg aagtggcggt
     4561 tcggcgcgcg gctgcagttc gtcaccggca atccgtatag cccgacgcca gcgggtgagg
     4621 agataccgat gccgatgccg ttcgccggcc ggctacctgc gttcttccac ctcgatgtcc
     4681 gcgtcgatcg cagctggcat cgctgctggg gcgacgtgaa tctgttcttc gatattcaga
     4741 acgtcacgaa tcgcacgaac gtcgaaggcc gcgagtacgg ctacaccgac gagcatccac
     4801 tcggcgccga cgaggacatt cctggtctcc cgatcgtgcc gttcat
//
LOCUS       DASUFX010000029         6531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_144516,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000029 DASUFX010000000
VERSION     DASUFX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144516"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFV99_01185"
     CDS             <1..745
                     /locus_tag="VFV99_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX5057934.1"
                     /translation="DGLDTRLMDIYGVSNNVAKPGVRLFEAWYEQPIGPFSIHAGLIA
                     ADQEFVLAEHSTVLLDATFGIVAMLSYNIGNPVYPVAAPGASLHYDSELVKLRAAFYA
                     NQVESHGIPQDVGGDGLFINEVEFLDMFKLGFWHHTTLGDGYYAIADKQLERYIGTFA
                     RVSYAPDSPLSVYADTGIRIGPGPLRKRDFASIGLAFARTEIGAQTIVEATYQYLVKG
                     WLTMQPDAQLLLTRTGTAGVFALRAVVAL"
     gene            772..1443
                     /locus_tag="VFV99_01190"
     CDS             772..1443
                     /locus_tag="VFV99_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057935.1"
                     /translation="MQGRKQSKRATAIALAFISAAALVVGGFGDRWLQTQEGIGFELN
                     DVPYRSGNIGLLSFAACQSECTSMSNTDLMPALDKWIATLKQRNQGAPVNVQQKVPYP
                     PWHGFPVVGIIALVAALLAAAGLVCGGLLALANKRMEMVVMPTTIAVLGLAISIINGC
                     LFVATKPDIGSMSVGWTFGAWGAGAVLGLAAVFPLNRAIRPIDEEIGVASATMSWGTS
                     RDDQP"
     gene            1513..1800
                     /locus_tag="VFV99_01195"
     CDS             1513..1800
                     /locus_tag="VFV99_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057936.1"
                     /translation="MERREELLAWIADTHRLQRKLAMVFPVLGVVSIALMFWRGAVGA
                     FALIVVALVAICSFWVTAAHNASHRQKLAELDRVARNEGKPLQTAHRRWHH"
     gene            complement(1797..5609)
                     /locus_tag="VFV99_01200"
     CDS             complement(1797..5609)
                     /locus_tag="VFV99_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5057937.1"
                     /translation="MRFVVPLLVFLGFCRTGHADTIISGGNLSTQTWTAANSPYTIQG
                     DVTVIAGATLTIEAGVVVQASSSSDAQASGRNTSRVEITVNGSLDVNGTAQQPVTFKS
                     TSTSSGTWYGLIATNMTSQFDLDNVVIESAIYGVTAEATGSQVNLTNVTASSASSYGF
                     YLRGGTPSLSAVKAISNGSYGVYISDTANPVIVGSTIRNSGSIGMYISHNTPGHSVTV
                     TNCTFNANGSYNIYSGASTGNAATINVKNSVLTNSSYGVYRNDSATWSVTYSNVWNNT
                     SGNYSGVSAGTGTFSSNPLYVSATDLRLTSNSPSRFGGDNNMDQGAAPYDTVATPGLY
                     GTLWSNTTLLASTGTYTAGGDLTVPAGVTLTIEPGVTLQFASSSDIMLSGTNTSRGEL
                     IVRGSLVANGTPTAPINLQSTSTSAGTWYGIELDTTSHDSVLDDLTIRYATYGVEYRS
                     TGTGNQLSNLTVESASSYGFYLRAGAPTLDMVSAISSGSYGYYISESSSPTFTRCIAR
                     NSGSIGMYISHNTPGRSVSLTRCTLNANGSYNVYSGASTGNGATITIVGSVLTNSSYG
                     VYRNDSATWTVTNSDVWNNTSGNYSGVSAGTGSISSNPLYVSTTDLRLTSNSPARFGA
                     PGNLDMGALPYDTVATPGLYGTLWSNTTLTAAGSPYTASGDLVIPPNTTLTIDPGVTL
                     QFASSSDIMLAGANTSRGELTVQGVLVADGTLQNPITIQSTSTSSGTWYGVDLDTGAH
                     ATTLDNVIVRYATYGLIYRSTGTGNTLKKITVESASSYGLYLRQGTPALDSVYVMSSG
                     SYGAYISDAASVTMTNSVIRNSGSIGMYISHNTPGRSVTVTNCTFNANGSYNIYSGAS
                     TGNAATINVSNSILTNSSYGAYRNDSATWTVSYSDVWNNTSGNYSGVSAGTGTISANP
                     QYLSTVDLHIMGTSVAIDAGTSGPNSDADGVARPLDGNGVGGAQWDMGAYEFVLMAQC
                     GNGLKELNESCDDGANNGNYGYCNTSCNGMGPRCGDNVKNGPEDCDDGNMSNLDECLT
                     TCTNPRCGDGYVRANVEACDDGNMSNTDACLNTCAAASCGDGYVRAGVEACDDGNQTN
                     TDACLSTCIVASCGDGFAQAGVEECDDGNMSNTDACLNVCKNASCGDGFVEMGKEACD
                     DGNMVDSDACNNSCAPVSCGDGVVQPGVEECDDANKIDSDACRSNCLTARCGDGAIQF
                     GVEECDDGNTTAGDGCDDMCLIEGDDMPPGMPRGDGGCCETSQGGGKSSVVLALVVML
                     GLRRRRRR"
     gene            5863..>6531
                     /locus_tag="VFV99_01205"
     CDS             5863..>6531
                     /locus_tag="VFV99_01205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5057938.1"
                     /translation="MRPAPTLSRIPAFPVITVVAAMAIFVTIAIKTGRTDIEPFTMSS
                     VAFDNEPWRLLSSALPHGDAIHLIFNVVWLWVLGTRLEETLGHITTFVIIIVLAVGSS
                     AAEFAFAAGGIGLSGVGYGLVGCLMVLARHDRRFRDAIDRNTLILFGAWFVLCVVLTL
                     ADVWNIGNVAHGSGFVLGVIIGYVIAPGTLARRIAAGGALVIVVAASLAGAALFRPQL
                     NLSAY"
BASE COUNT         1030 a         2154 c         2113 g         1234 t
ORIGIN      
        1 cgacggtctc gacacgcgcc tcatggacat ctacggcgtg tcgaacaacg tcgcgaagcc
       61 gggcgtgcgc ctgttcgagg cctggtacga gcaaccgatc ggcccgttca gcatccacgc
      121 cggtttgatc gcagcggacc aggagttcgt gcttgccgag cacagcaccg tgttgctcga
      181 cgcgacgttc ggcatcgtcg cgatgctcag ctacaacatc ggcaacccgg tctatccggt
      241 cgcggcaccg ggcgcgagcc ttcactacga cagcgagctc gtgaagctgc gcgccgcgtt
      301 ctacgcgaac caggtcgagt cgcacggcat tccgcaggac gtcggcggcg acgggctctt
      361 catcaacgag gtcgagtttc tcgacatgtt caagctcggc ttctggcacc acacgacgct
      421 tggcgatggc tactacgcga tcgcggacaa acagctcgag cgttacatcg gcacgttcgc
      481 gcgcgtcagc tatgcacccg acagcccgct ctccgtctat gccgacaccg gcatccgcat
      541 cggcccgggc ccgctgcgca agcgcgactt cgccagcatc gggctcgcat tcgcacggac
      601 cgagatcggt gcgcagacaa tcgtcgaggc gacgtaccag tacctcgtca agggctggct
      661 cacgatgcag cccgacgcgc agctcctgct cacgcgcacg ggcacggccg gtgtcttcgc
      721 gctccgcgca gtggtcgcgc tctgactgct cgcgttacga tgctgccttc gatgcaaggg
      781 cgtaaacaaa gcaaacgtgc gaccgcgatc gcgctcgcgt tcatctcggc agcggcactc
      841 gtcgtcggcg gattcggtga tcgctggctg cagactcagg agggcatcgg cttcgagctc
      901 aacgacgtgc cctatcgatc gggcaacatc ggcctgctca gctttgcagc ctgccagagc
      961 gagtgcacga gcatgtcgaa caccgacctc atgcctgcgc tcgacaaatg gatcgcgacg
     1021 ctaaagcagc gcaatcaggg cgcaccggtc aacgtacagc aaaaggtgcc ctacccgcca
     1081 tggcacgggt ttcctgtcgt cggcatcatc gcgctcgtcg cggcgctgct cgcagccgcg
     1141 gggctcgtgt gcggcggcct gctcgcgctc gcgaataaac gcatggagat ggtggtgatg
     1201 ccgacgacga tcgcggtcct cggtctcgcg atcagcatca tcaatggctg cctgttcgtc
     1261 gcgaccaagc cggatatcgg atcgatgagc gtcggctgga cgttcggcgc gtggggtgcc
     1321 ggcgccgtcc tcggccttgc cgccgtgttc ccactcaatc gtgcgattcg gccgatcgac
     1381 gaagagatcg gtgtggcctc ggcgacgatg tcgtggggta cgagccgcga cgatcagccc
     1441 taggccgagc cctgcgaccg cacgccgcag tccgatcagc cgccagaccg catcggagct
     1501 acacttgcca tgatggagcg ccgagaagag ctgctcgctt ggatcgcgga cacgcatcgt
     1561 ttgcagcgca agctcgcgat ggtgtttccg gtgctcggcg ttgtctcgat cgcgctgatg
     1621 ttctggcgcg gagccgtcgg cgcgttcgcc ttgatcgttg tcgcgctggt cgcgatctgc
     1681 tcgttctggg tcaccgccgc acacaacgca tcgcatcgcc agaagctggc ggagctcgac
     1741 cgcgtcgcac gcaacgaagg caagccgcta cagaccgcgc accgccgctg gcaccactaa
     1801 cggcgccgac gccggcgcaa gccgagcatc acgacgagcg cgagcaccac gctgctcttg
     1861 ccaccgccct gtgaggtctc gcaacagccg ccatcaccgc gcggcatgcc cggcggcatg
     1921 tcatcgccct cgatgaggca catgtcgtcg caaccgtcgc ctgcagtggt gttgccgtcg
     1981 tcgcactcct cgacgccgaa ctggatcgcg ccgtcgccgc accgtgcggt caagcagttg
     2041 ctgcggcacg cgtcgctatc gatcttgttt gcgtcgtcgc actcctcgac acccggttgc
     2101 acgacgccat cgccgcacga cacgggcgcg cagctgttgt tgcacgcatc gctgtcaacc
     2161 atattgccgt cgtcgcacgc ttccttgccc atctcgacga agccgtcgcc gcagctcgcg
     2221 ttcttgcaga cgttgaggca cgcgtcggtg ttcgacatgt tgccgtcgtc gcactcctcg
     2281 acacccgctt gcgcgaagcc atcgccacag ctcgcgacga tgcacgtgct caagcacgcg
     2341 tcggtgttcg tctggttgcc gtcgtcgcac gcctcaacac cggcgcgcac gtagccgtcg
     2401 ccgcagctcg cagctgcgca ggtgttgagg cacgcgtcgg tgttcgacat gttgccgtcg
     2461 tcgcacgcct cgacgttcgc gcgcacgtag ccgtcaccgc agcgcgggtt cgtgcaggta
     2521 gtgaggcact cgtcgaggtt cgacatgttg ccgtcgtcgc agtcctcggg accattcttg
     2581 acgttgtcgc cgcagcgcgg gcccatcccg ttgcagctcg tgttgcagta gccgtagttg
     2641 ccgttgttcg caccatcgtc gcagctctcg ttgagctcct tgaggccgtt tccgcattgc
     2701 gccatcagca cgaactcgta cgcgcccatg tcccattgcg caccaccgac gccgttaccg
     2761 tcgagtggcc gcgcgacacc gtccgcgtcg ctgttcgggc cgctcgtgcc tgcatcgatc
     2821 gcgacgctcg tgcccatgat gtggaggtcg acggtcgaga ggtactgcgg gttcgccgag
     2881 atcgtgccgg tgcccgcgct gacccccgag tagttgccgc tcgtgttgtt ccagacgtcc
     2941 gagtagctga cggtccacgt tgcgctgtcg ttgcggtagg cgccgtacga cgaattcgtg
     3001 aggatcgagt tcgagacgtt gatcgtcgcc gcgttgcccg tcgatgcgcc cgagtagatg
     3061 ttgtacgagc cgttcgcgtt gaacgtgcag ttcgtgaccg tgaccgagcg ccccggcgtg
     3121 ttgtgcgaga tgtacatgcc gatgctgccg ctgttgcgaa tgaccgagtt cgtcatcgtc
     3181 accgacgctg catcgctgat gtacgcgccg tagcttccgc tgctcatcac gtacaccgag
     3241 tcgagcgccg gcgtgccctg gcgcagatac aggccgtacg acgacgccga ctcgacggtg
     3301 atcttcttca gcgtgttgcc ggtgccggtc gagcgataga tgaggccgta ggtcgcgtag
     3361 cgcacgatca cgttgtcgag cgtggtcgcg tgtgcgcccg tgtcgagatc gacgccgtac
     3421 caggtgccgg acgatgtcga ggtcgactgg atcgtgatcg gattctgcag cgtaccgtcg
     3481 gcgacgagca cgccctgcac ggtcagctca ccgcggctcg tgttcgcgcc ggcgagcatg
     3541 atgtcgctcg agctcgcgaa ctgcagcgtg acgcccggat cgatcgtgag cgtcgtgttc
     3601 ggcgggatca cgaggtcgcc gctcgccgtg taggggctgc cagccgcggt cagcgtcgtg
     3661 ttcgaccaca gcgtgccgta gaggccaggc gtcgcgaccg tgtcgtacgg caacgcgccc
     3721 atgtcgaggt tccccggcgc gccgaagcgt gccggcgagt tcgacgtcag tcgcaaatcc
     3781 gtcgtcgaca cgtagagcgg gttcgacgag atcgagccgg tgcctgccga cacgccgctg
     3841 tagttgccac tcgtgttgtt ccagacgtcc gagttggtca ccgtccatgt agcgctgtcg
     3901 ttgcgataca cgccgtacga cgagttcgtg aggaccgagc cgacgatcgt gatcgtggcg
     3961 ccgttgccgg tcgacgcccc ggaatacacg ttgtacgagc cattcgcgtt cagcgtgcag
     4021 cgcgtgagcg agaccgagcg gcccggcgtg ttgtgcgaga tgtacatgcc gatgctgccg
     4081 ctgttgcgcg cgatgcaacg cgtgaacgtc ggcgacgacg actccgagat gtagtagcca
     4141 tagctgccgc tgctgatcgc gctcaccatg tcgagggtcg gcgcgccagc gcgcaggtag
     4201 aagccatacg acgacgcgct ctcgacggtg aggttcgaga gctggttgcc agtgccggtc
     4261 gagcggtact cgacaccgta ggtcgcgtag cgaatcgtga gatcgtcgag cacgctgtca
     4321 tgcgacgtcg tgtcgagctc gatgccgtac caggtgccgg ccgacgtcga ggtggactgg
     4381 agattgatcg gtgccgtcgg cgtgccgttc gcgacgagcg agccgcgcac gatgagctcg
     4441 ccgcggctgg tgttcgtgcc cgacaacatg atgtcgctcg agctcgcgaa ctgcagcgtc
     4501 acgccgggtt cgatcgtcag cgtcacgccg gcaggcaccg tgaggtcacc gcccgcggtg
     4561 tacgtgcccg tcgatgcgag cagcgtcgtg ttcgaccata gcgtgccgta gagacccggc
     4621 gtcgcgaccg tgtcgtacgg agccgcgccc tgatccatgt tgttgtcacc accgaaccgc
     4681 gacggcgagt tcgacgtcag ccgcagatcc gtcgccgaca cgtagagcgg gttcgacgag
     4741 aacgtgcccg tgcctgccga cacgccggag taattgccgc tcgtgttgtt ccagacgttc
     4801 gagtacgtga cgctccaggt cgcgctgtcg ttgcggtaca cgccatacga cgaattcgta
     4861 agcaccgagt tcttgacgtt gatcgtcgcg gcattgccgg tcgatgcacc ggagtagatg
     4921 ttgtacgagc cgttcgcgtt gaacgtgcag ttcgtgacag tgacggagtg gcccggcgtg
     4981 ttgtgcgaga tgtacatgcc gatgctgccg ctgttgcgga tcgtcgagcc gacgatcacc
     5041 gggttcgcgg tgtcactgat gtacacgccg tagctgccgt tgctgattgc cttcacggcc
     5101 gagagactcg gcgtgccgcc gcgcaagtag aagccatacg acgacgccga cgatgcggtg
     5161 acgttcgtca ggttgacctg cgaacccgtc gcttcggcag tgacaccata gatcgccgac
     5221 tcgatgacca cattgtcgag gtcgaactgc gacgtcatgt tggtcgcgat caggccgtac
     5281 caggtgcccg acgacgtcga tgtcgacttg aacgtcaccg gctgctgcgc ggtgccattg
     5341 acgtcgagcg agccattgac cgtgatctcg acccggctcg tgttgcgccc cgacgcctgc
     5401 gcgtccgagc tcgacgacgc ctgaacgacg acgccggctt cgatcgtcag cgtagcgccg
     5461 gcgatcaccg tgacatcgcc ctggatcgtg tacgggctgt tcgccgccgt ccacgtctgg
     5521 gtgctgaggt tgccgccgct gatgatcgtg tcggcgtggc cggtgcggca gaagcccagg
     5581 aacacgagaa ggggaacaac gaagcgcata gttggcgacc tcaaggaacg gaccctttgg
     5641 aagacgagcc gccgcccgtg cggaggtcgc acggaatcga cggggccccg ttcagcaagc
     5701 gacgtgcccg gctaaggccg cgacgccacg tacccggcgg caccactgca ctgctgcacc
     5761 accgttgcag ccgcgtgcag cgcttcgtgc ccggcccggt caggaattga gctgcgctcg
     5821 cgaaatagac gccttcgtgc cctcattata ggctgcaccg ccgtgcggcc ggcccccacc
     5881 ctgtctcgca ttccagcgtt tccagtcatc acggtggtcg ctgcgatggc gatcttcgtg
     5941 acgatcgcga tcaagaccgg ccgcaccgac atcgagccgt tcacgatgag ctcggtcgcg
     6001 ttcgacaacg agccgtggcg gctgctctcg agcgcgctac cacacggcga cgcgattcac
     6061 ctgatcttca acgtcgtctg gctgtgggtg cttggcacgc ggctcgaaga gacgctcggt
     6121 cacatcacga cgttcgtgat catcatcgtg ctcgccgtcg gctcgtcggc cgccgagttc
     6181 gcgttcgctg ccggtggcat cggcctctcg ggcgtcggct acgggctcgt cggctgtctc
     6241 atggtgctcg cgcggcacga ccggcggttt cgcgatgcga tcgatcgcaa cacgctcatc
     6301 ctgttcggcg cctggttcgt gctctgcgtc gtgctcacgc tcgccgacgt ctggaacatc
     6361 ggcaacgtcg cgcacggcag cggcttcgtg ctcggcgtga tcatcggcta cgtgatcgca
     6421 cctgggacgc tcgcgcgccg catcgccgct ggcggtgcgc tcgttatcgt cgtcgctgca
     6481 tcgctcgccg gtgctgcgtt gttccggccg caactcaacc tgtccgccta c
//
LOCUS       DASUFX010000030         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1672227, whole genome shotgun sequence.
ACCESSION   DASUFX010000030 DASUFX010000000
VERSION     DASUFX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672227"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1061)
                     /locus_tag="VFV99_01210"
     CDS             complement(183..1061)
                     /locus_tag="VFV99_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5057939.1"
                     /translation="MRTWKIGDVEITRIVEVNNWEDDITMLLPDATPATVLAYPWLQP
                     HYATPQGKMIISFQCFVMRTREHAIMLDTCIGSDRKREFDIFTNMRSDFLADLEHAGF
                     GPEEIDLVLCTHLHFDHVGWNTRLVDGKWVPTFPNARYLFGRREYEHWKMLKETHGYH
                     DLNHMEDAVQPVIDAGLVDLIDPGHSICPEIKLRPTPGHTPGHVSIGIESRGKRALVT
                     GDMMHNTIQLERPDDTARFDMDQVMARRTRVKLIDDLANSDVFVIGSHFSEPTGGYIV
                     HDTKGCRLAQQPAGKK"
     gene            1229..1618
                     /locus_tag="VFV99_01215"
     CDS             1229..1618
                     /locus_tag="VFV99_01215"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HEX5057940.1"
                     /translation="MGIVSAKLRLSNPRNPSATPIEVGALADNGSVFLCIPQHVCLQL
                     GLDVLQEREVKLADGSCISVPYVGPVLVQFKNRTGVMGALVLGDEVLLGAIPMEDMDL
                     VVNPRDRTVDVNPASPNIASGIVKRAA"
     gene            complement(1628..3277)
                     /locus_tag="VFV99_01220"
     CDS             complement(1628..3277)
                     /locus_tag="VFV99_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5057941.1"
                     /translation="MSSTRTEFDREFDYIVVGAGTAGCELAARLSEDARNCVCLIEAG
                     GSNRHPYIAIPAAVGCAIMSPKFGWGLSTVPQPHLGGRRVPLPRGRVIGGTGSINGMA
                     YFRGPARDYDDWAAMGNPGWSYADLLPYFLRSEHNPEYAGSPYHATGGPMGVSFPTSR
                     NPLCDAFNAAMASLGYSELPDFNVPDPNGYGYRQGTIWKGRRVSTASAYLKPALGRRN
                     LEVRSETRVRRVLLEGKRAVGVEIQGSDGRIQRLRAGREVVISCGTFHSPHLLLHSGI
                     GHERDLEKWGITPLHHLPAVGHHLRDHPAAPIAMESDDATSYGHSWKALPRNVAQTLR
                     YLATRTGQYASNLFETNAYVRTLPDSDRPDLQIVFQPARRNTKPFPIPLGHGYAIVIV
                     CLYPRSMGRVSLSGPDPFALPVIDPKLGSDEVDLQTLVRGLKVARKIFAHESFRRYRA
                     QEVFPGAAVASDEQWLEHIRATLTTVHHPASTCRMGPAGDNVVDHELKVHGLEQLRVA
                     DASIYPRLVGANTNASVVAIAEKASDMILGRPAPAPITQRA"
     gene            3323..4516
                     /locus_tag="VFV99_01225"
     CDS             3323..4516
                     /locus_tag="VFV99_01225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5057942.1"
                     /translation="MELRTQLEARGSTYPLGGSYAPEYQGVVDAFVENFRVEDEIGAA
                     CSIMLDGRTVVDLWGGWRDGAKKTAWDAPTTVCMMSVAKGITAICFNMLIDRGLVDPK
                     ARVIDYWPEYGRNGKEKTLVRHFLDHTAAVPVLTTNPLWPGAMFDREAIVKALEEQEP
                     LWEIGTKAAYHVHHQGFLLGEIMRRVTGKTVGPFLRDEVATPLKAEYYIGGMTDEQQS
                     FVAEVMPNMEAKLFAAKDGPDQTSLRALAFKQNPAEPWAVTMNRREWRTVEVASGSGH
                     GNARGVARIYGALGAGGALDGVKIMSKRQIEAMTTEQHHQTEALQDRPYHQALGLLLN
                     TPEAVYMGPNLRSFGHHGIGGSIGFCDPDARMGFSYACNKMHAVGTNGPRAARLIDAF
                     YRCEL"
     gene            complement(4498..>4754)
                     /locus_tag="VFV99_01230"
     CDS             complement(4498..>4754)
                     /locus_tag="VFV99_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057943.1"
                     /translation="ETVPKTLAASSMGLVVGAGEIVGGVLSPTISGWIADQTSLVAHM
                     VVMMVCALCGGLLSLFLRETAPGKVKTAELTPEPAYSSQR"
BASE COUNT          842 a         1545 c         1550 g          817 t
ORIGIN      
        1 gatgattccc ttgtgacccg tgtgcacacc ggcgcccgga atatccgagt tactcatcgg
       61 cgtccacgtc gcctgcggat gcaggatcgt gcgcaagcct tcgaggtcct ccgcgttcag
      121 cttcgcgaag aactgccgca acaacgcttc attcggcgag tccgccatgg atgatcctcc
      181 ttctacttct tgccggccgg ttgctgggcg agcctgcagc ctttggtatc gtgcacgata
      241 tagccgcccg tcggctcgct gaagtgcgag ccgatgacga acacgtcgga attggcgaga
      301 tcgtcgatga gcttcacgcg cgtgcggcgc gccatgacct gatccatgtc gaagcgcgcc
      361 gtgtcgtctg ggcgctcgag ctggatcgtg ttgtgcatca tgtcccccgt gaccagcgca
      421 cgcttgccgc gcgactcgat gccgatactg acatgccccg gcgtatgacc gggcgtcggg
      481 cgcagcttga tctcggggca gatcgaatgg ccgggatcga tcaggtcgac cagaccggcg
      541 tcaatgacgg gttgcacggc atcctccatg tgattgaggt cgtgatagcc gtgcgtttcc
      601 ttgagcatct tccagtgctc gtactcgcgc cgcccgaata gatagcgcgc gttcgggaac
      661 gtgggcaccc acttgccatc gacgagccgc gtgttccagc cgacgtggtc gaagtgcaaa
      721 tgcgtgcaga gcacgagatc gatctcttcc ggcccgaacc cggcatgctc gaggtcggcg
      781 aggaaatcgc tgcgcatatt ggtgaagatg tcgaactcgc gcttgcgatc cgagccgatg
      841 caagtgtcga gcatgatcgc gtgctcgcgc gtgcgcatca cgaagcactg gaacgagatg
      901 atcatcttgc cttgtggcgt cgcgtagtgc ggctgcagcc acggatacgc gagcacggtt
      961 gccggcgtcg catccggcag cagcatcgtg atgtcgtctt cccagttgtt gacctcgaca
     1021 atgcgcgtga tctcgacgtc gccgatcttc caggtgcgca tggttgcgat ggacatcctt
     1081 ccccctgcac gcgccgccgc gttgcaacga tttcggaccg gcgacgagcg attgccgcct
     1141 agtgtacgga ttacggtcgc gcttttccgg ttgatgcgac cgtaaacgcg cgtggtagcc
     1201 ttcgaatgac tgaccgtaga ttggaggcat gggaatcgtg tccgccaaac tcagactgag
     1261 caacccgcga aacccttcgg ccacgccgat cgaagtcggc gcgctcgccg ataacggctc
     1321 cgtgttcctc tgcatcccgc agcacgtgtg cctccagctt gggctcgacg tgttgcagga
     1381 gcgcgaggtg aagctcgcgg acggttcctg catcagcgtt ccctatgtcg gcccagtgct
     1441 cgtgcagttc aaaaaccgaa ccggcgtcat gggcgcgctg gtgctcggtg acgaggttct
     1501 gctcggggcg attccgatgg aggacatgga tctcgtcgtc aatccgcgcg atcgcaccgt
     1561 cgacgtgaat cccgcgagcc cgaatatcgc gtccgggatc gtcaagcgcg cggcctgaga
     1621 aagccgctca cgctcgttgc gtgatcggcg ccggcgccgg cctacccaag atcatatcgg
     1681 acgccttctc ggcgatcgct accacgctgg cattcgtgtt cgctccaacg aggcgcggat
     1741 agatcgacgc atcggcgacg cgcagctgct cgagcccgtg caccttcagc tcgtggtcga
     1801 cgacgttgtc gccggccggg cccatgcggc acgtcgaggc aggatgatgg accgtcgtca
     1861 gcgtggctcg gatatgctcg agccattgct cgtcggacgc caccgcggct ccggggaaca
     1921 cttcctgcgc gcgataccgt cggaagcttt cgtgcgcgaa gattttgcgc gccaccttca
     1981 agccgcgcac gagcgtctgc agatcgacct cgtcgctgcc gagcttggga tcgatgaccg
     2041 gcagcgcgaa cggatccggc ccggacaggc tcacgcgccc catgctgcgc ggatagaggc
     2101 acacgatgac gatcgcgtag ccgtggccga gcggaatcgg gaatggcttc gtgttgcgcc
     2161 gcgcgggctg gaacacgatc tgcaagtcgg ggcgatcgga gtccggcaac gtgcggacgt
     2221 aggcgttcgt ctcgaacagg ttgctcgcgt actgacccgt gcgcgtcgcg agatagcgca
     2281 acgtctgcgc gacgttgcgc ggtaacgctt tccacgagtg gccatacgac gtggcatcgt
     2341 cggactccat cgcgatcggc gccgccggat ggtcgcgcag atggtggccg accgccggca
     2401 ggtgatgcag cggcgtgatg ccccacttct cgagatcgcg ctcgtggccg atgccggagt
     2461 gcagcagtaa gtgcggcgag tggaacgtgc cgcaactgat cacgacttcc ctgcccgcgc
     2521 gcagccgctg aatgcggccg tcgcttccct ggatctcgac cccgaccgcg cgtttgccct
     2581 cgagcagcac gcgccgcacg cgcgtctcgc tccgcacctc gagattgcgc cgaccaaggg
     2641 cgggcttcag gtacgcgctc gccgtcgaga cgcgccggcc cttccagatc gtgccttgcc
     2701 gatagccgta gccgttggga tccggaacgt tgaagtcggg caattcggaa tagccgagcg
     2761 acgccatggc cgcgttgaat gcatcgcaaa gcgggttgcg gctcgtcgga aagctcacgc
     2821 ccattggccc gcccgtcgcg tgatacggcg agcccgcgta ctcggggttg tgctcggagc
     2881 gcaggaaata cggcaacaga tcggcgtacg accagcccgg attgcccatg gcggcccagt
     2941 cgtcgtaatc cctcgcgggt ccgcggaagt aggccatacc gttgatcgag cccgtgccgc
     3001 caatcacgcg accgcgcggc aacggcacgc ggcggccgcc caaatgcggc tgcggcacgg
     3061 tcgaaagccc ccagccgaac ttcgggctca tgatcgcgca gcccacggcg gcgggaatcg
     3121 cgatgtacgg atggcggttg gagccaccgg cttcgatcaa gcagacgcag ttgcgcgcgt
     3181 cctccgacag gcgcgccgcg agctcgcacc cggccgtgcc ggcgccgacg acgatgtagt
     3241 cgaactcgcg atcgaactcg gttcttgtgc tgctcaagca ggtcggccca aacttacgcg
     3301 cgcagtatag cgaggaaatt cagtggagct ccgtacgcaa ctcgaggcgc gcggctcgac
     3361 gtatccgctc ggcggctcgt acgcccccga gtaccagggc gtcgtcgacg cgttcgtcga
     3421 gaacttccga gtcgaggacg aaatcggcgc ggcctgctcg atcatgctcg acgggcgaac
     3481 ggtcgtcgac ctctggggcg gatggcgcga cggtgccaag aagaccgcgt gggatgcgcc
     3541 gacgacggtc tgcatgatgt cggtcgcgaa aggcatcacg gcgatctgct tcaacatgct
     3601 catcgaccgc ggcctcgtcg atccgaaagc acgcgtcatc gactattggc cggagtacgg
     3661 gcgcaacggc aaagaaaaaa cgctcgtgcg gcacttcctc gaccacacgg ccgccgtgcc
     3721 ggtgctgacg acgaatccgc tctggccggg cgcaatgttc gatcgcgaag cgatcgtcaa
     3781 agcactcgag gaacaagagc cgctctggga gatcggcacc aaagccgcgt atcacgtgca
     3841 tcatcagggc ttcctgctcg gcgagatcat gcgccgcgtc accggcaaga cggtcgggcc
     3901 gttcttgcgc gacgaagtcg cgacgccgct caaagccgag tactacatcg gcggcatgac
     3961 cgacgagcag cagtcgttcg tcgccgaggt gatgccgaac atggaggcga agctgttcgc
     4021 cgcgaaggac ggccccgatc aaacctcgct gcgcgcgctc gcgttcaaac agaaccccgc
     4081 cgagccgtgg gccgtgacga tgaaccggcg cgaatggcgc acggtcgagg tcgcgagcgg
     4141 cagcggccac ggcaacgcgc gcggcgtcgc acgaatttac ggcgcgctcg gcgcgggcgg
     4201 agctctcgac ggcgtgaaga tcatgagcaa aaggcagatc gaggcaatga cgaccgagca
     4261 gcaccatcag accgaggcgc tacaagaccg gccgtaccat caagcgctcg ggctgctgct
     4321 gaatacgccg gaagccgtct acatggggcc gaacctgcgc agcttcggtc atcacggcat
     4381 cggcggctcg attggcttct gcgatcccga cgcgcgcatg ggcttctcgt acgcgtgcaa
     4441 caagatgcac gcggtcggca cgaacgggcc gcgcgccgcg cggctgatcg acgcgttcta
     4501 ccgctgcgag ctgtaggcgg gctcgggcgt cagctcggct gtcttcactt ttcccggcgc
     4561 cgtctcgcgt aggaacaggc tcaacaggcc gccgcacagc gcgcacacca tcatgacgac
     4621 catgtgcgcg acgagcgacg tctgatccgc gatccagccc gagatcgtgg gcgacaacac
     4681 gccgccgacg atctcgccgg cgccgacgac caatcccatc gacgacgcgg ccagcgtctt
     4741 cggcacggtc tccg
//
LOCUS       DASUFX010000031         8305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1255621, whole genome shotgun sequence.
ACCESSION   DASUFX010000031 DASUFX010000000
VERSION     DASUFX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8305
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255621"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFV99_01235"
     CDS             <1..249
                     /locus_tag="VFV99_01235"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HEX5057944.1"
                     /translation="KADDEIDMTIVPQILGGRVHITAVYQLADIAPGKVRRRYQGSIT
                     VQASLLSGKIERAIAAEIEKGMPVMTGCTQSWLTSAGI"
     gene            351..776
                     /locus_tag="VFV99_01240"
     CDS             351..776
                     /locus_tag="VFV99_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5057945.1"
                     /translation="MTLDKLFLSLHVVGALMWIGSLFALAAFLEAFADETDPAAKSRL
                     LKHLRQAAIVPDVGATLAIAFGLHWLFKFKLYQMPYMHIKLALVAGVIGMHGYLKVKA
                     KKARKGEAFTAPPMAMKPMLSLLALGIILFVIEKWPLPS"
     gene            complement(769..1809)
                     /locus_tag="VFV99_01245"
     CDS             complement(769..1809)
                     /locus_tag="VFV99_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5057946.1"
                     /translation="MPRQSERYALDLSLDLYASREWRHAAMQDISRTGMFIATSPILV
                     GTPVLVSLEHNGTRVTNPARVMHTITSDEARALCRVPGIGLQFHEPVDVAFAVAIENL
                     MRRARTKQPQQFHMVVADSELRVLERLSTALDAAGFSVATASNGLEVFGACLRQKPDV
                     VLVDRATPMVDGFQLIERLATDDRMSSVPVVVTTNDPSDIERAFQRGAADVILKPFAL
                     VEAIARVRRVAETPKRAERVLLTGSISELGIASVLTMMEIEKKTGRLVASNGHAAWID
                     IVDGRIVDAGWSLGTSHPRAVVMSVLDWTQGSFKLVAVPTRHSDSDLAMPVTHLILEQ
                     ARLRDEAARAIS"
     gene            complement(1901..2164)
                     /locus_tag="VFV99_01250"
     CDS             complement(1901..2164)
                     /locus_tag="VFV99_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057947.1"
                     /translation="MTEVAPSDLMAVIGGADPSFGRCGPGSSWKILGDVRTPECAAHD
                     QAVQTAIQNGHSYLGAQLQALPKLGPAIGSYFRERFKGSGSAG"
     gene            2305..2523
                     /locus_tag="VFV99_01255"
     CDS             2305..2523
                     /locus_tag="VFV99_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057948.1"
                     /translation="MESAMIDLVRELDDPTRRRDPELEVDIEIDDMLLDPWFLMSEDE
                     IAELDDDPNASVLEIRLAPRVDQLDYVA"
     gene            complement(2532..4076)
                     /locus_tag="VFV99_01260"
     CDS             complement(2532..4076)
                     /locus_tag="VFV99_01260"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEX5057949.1"
                     /translation="MIDCNGSVVEDCTGGQACDPSTYTCQDACAAAEANHRSVGCDYF
                     ATEMETLTPGYCYAVFVANTWTFPAHIDVEYRGQPLNTQTFTRLPTGAGSSLTYGTYD
                     ATQGLAPGEVAILFLAGSQGSAPNCPVTPAVPSAYFTGTGIGDSFHIKTDVPVVAYQI
                     NPYGGGSVAVTAASLLLPTSVWDMNYVAVNVSPEAPSVGHPSLNIVAREDGTQVMLTP
                     KQAVTAGTGIPGAAAGQPIMISLNKGQNAQITQPAELTGSVVQANKPVGFMAGHTCMQ
                     VPVGTAYCDHGEQMVPPVKALGSRYVGVMYKPRVPSETSTYWRMVGAVDGTTLTYSTS
                     VGGPTTINAGQEVTFQTGIPFTVQSQDSEHPFMLFTYMASSTAVSTGYGDPDFVTSVP
                     PEQYLRQYVFFTDPTYPETNLVVVRSRGTDNNFHDVQLDCAGVLSGWTGIGGEFEYTR
                     VDLSTGNFTAVGQCNNGRHEIKSDAPFGLWVWGWGTPLTSTFTQNVSYGYPGGMNVAP
                     INTVIF"
     gene            4422..5729
                     /locus_tag="VFV99_01265"
     CDS             4422..5729
                     /locus_tag="VFV99_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5057950.1"
                     /translation="MGIAKSVLPTYFDVAIIGAGPVGLAIAIELARLDLTTLVVDRRP
                     PPAEDTGLRPQLLVARAGDLANLAHLGVDVRDPRIVTLLASRCEADLATGRTIRGGVA
                     TLHSVPPQLDLWTLAAQPPRALVPIGRLQQALLERALHHGASVHYGCEVTRVRRHARF
                     ASLTLADGVAVRAALVVIATGAARSLALELLRGACAVELPEQRIIGALFDAAADEGRW
                     IRVELPVPGLPRLARATVLQTTDAGTGVLVSAPGQPTAEQLGRCFITAARAHGLLGAP
                     MLVEPQIFATAVTAMARRTIAGDGRAPIVIAGDAAQTGHVFTGQTCFVNVALALRMAR
                     ELQHARRAIALQLAGDAALLRALDRYEQQSQIGAAILARTSQRHYAPHRSGQWALAGV
                     ARANGLARRAFRLGGPFVFRLPLRAIKDQWPVTKSARGGRATA"
     gene            5684..6124
                     /locus_tag="VFV99_01270"
     CDS             5684..6124
                     /locus_tag="VFV99_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEX5057951.1"
                     /translation="MASYEKRTWWTRHGLTDRLASDTFLEAAVTSAFLIAAADGNASE
                     QEYDALLDRLEILGGVDRDKIDELLTSVSNDIEANGFEGRIARVAEIVGDRSAAEAAL
                     MLGLAIGLADNDFSAEEREVANQLATAIGVGADDIDQRVAEIRG"
     gene            6269..7339
                     /locus_tag="VFV99_01275"
     CDS             6269..7339
                     /locus_tag="VFV99_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057952.1"
                     /translation="MRIAIASLLLVGCVAAQDPSGSDDDDRGSGSGSGSDMGTGSGSG
                     SGSGSGSGSAQPPVPVASGAYQVRSDIDLTVEALLPEPVENIVLTLRDFSMNPAETLF
                     DLAEDIGVPAVGEIRTALPDYLEDKALGWLNTEINKVTVGGVPVPQVAGNIAALAETA
                     LTKFALDSELTITGQTATHTLKTLDLTPAGINVQVALEALPTEIIAATPSCSSSMGTV
                     ALGDHSYSLQYGEYMWRAINLAVTAQYGTDVHGVIADAVNCQAIAHTVASKCYLGVCV
                     GHESQLTDICEAGVNEVVERVHDKFSEMRFDAIHFAAGTAALVDANHDGDAEAMTMGV
                     WMAEINAGLGLRHVPATFTATK"
     gene            complement(7323..8126)
                     /locus_tag="VFV99_01280"
     CDS             complement(7323..8126)
                     /locus_tag="VFV99_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057953.1"
                     /translation="MSLVVSRAFGLCTVQDLGRRGHMHEAVPPGGALVRELLVAANRS
                     VGNADDAPAIEVLGQLIVNATAEIMVATDRTSPQLLAAGDTLTIESEPLRCTYLAIAG
                     GIAAPRILDGRGTLLCARLGAPLRAGATLSPAEHLATDAHALASFTATDVIQVLPGPD
                     LDAFADNVLGQLVGTPYRISPSSDRVGTRLEGAALPRLATYREQSRPMVMGALEVPGD
                     GVPIVLGPEHPTTGGYPIVAVIANAELGRFHAIRLGGTVQFALATTSSR"
     gene            complement(8123..>8305)
                     /locus_tag="VFV99_01285"
     CDS             complement(8123..>8305)
                     /locus_tag="VFV99_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX5057954.1"
                     /translation="RATPRPRVPAGSLAIADAFTAVYPFDSPGGWHLIGRVVETTMFD
                     ERGALLQLGDRVRFVS"
BASE COUNT         1409 a         2691 c         2771 g         1434 t
ORIGIN      
        1 aaagccgacg acgagatcga catgacgatc gtcccgcaga tcctcggcgg gcgggtgcac
       61 atcacagccg tgtatcagct cgcggacatc gcgcccggca aagtgaggcg taggtaccag
      121 gggtcgatca cggtccaagc atcactactg tccggaaaga tcgagcgcgc gatcgctgcc
      181 gagatcgaga agggcatgcc ggtgatgacc ggctgcacgc agagctggct gacgagcgcc
      241 ggcatctaga cgtagcgtct actacgtcag acatattcgg cgcgcgcagc tagcgctgtc
      301 aggaatcccg acagccgcat gcgcacaaaa gcggtgctac tgtctggcgc atgacgctcg
      361 acaagctgtt cctgtcactg cacgtcgtcg gggcactgat gtggatcggc agcctgttcg
      421 cgcttgccgc gttcctcgag gccttcgccg acgagaccga tcccgcagcg aagagccggt
      481 tgctcaagca cctgcgccag gctgcgatcg tacccgacgt cggcgcgacg ctcgcgatcg
      541 cattcggcct gcactggctg ttcaagttca agctctacca gatgccgtac atgcacatta
      601 agctcgcgct cgtcgcgggc gtgatcggca tgcacggcta tctcaaagtg aaagcgaaga
      661 aggcgcgcaa gggcgaggca ttcaccgcgc cgccgatggc gatgaagccg atgctgtcgc
      721 tgctcgcgct cggcatcatc ctgttcgtga tcgagaagtg gccgctgcct agctgatcgc
      781 gcgcgccgct tcatcgcgca gccgcgcctg ctcgaggatc aggtgcgtca ccggcatcgc
      841 gaggtcgctg tccgagtggc gcgtcggcac cgcgacgagc ttgaacgagc cctgcgtcca
      901 gtcgagcacc gacatcacga ccgcacgcgg atggctggtg ccgagtgacc agccggcgtc
      961 gacgatgcgg ccgtcgacga tgtcgatcca tgcagcgtgg ccgttcgaag cgacgagtcg
     1021 gcccgtcttc ttctcgatct ccatcatcgt gagcaccgac gcgatgccga gctcgctgat
     1081 cgagccggtg agcagaacgc gctcggcacg cttcggcgtc tccgcgacgc ggcgtacgcg
     1141 tgcgattgcc tcgacaagcg cgaacggctt caggatgacg tcggcagcac cgcgctgaaa
     1201 cgcacgctcg atgtcgctcg gatcgttcgt cgtcacgacg accggcaccg atgacattcg
     1261 atcgtcggtc gcgagccgct cgatcagctg aaacccatcg accatcggcg tcgcgcgatc
     1321 gacaagcacg acatccggct tctgccgcag acacgcgccg aacacctcga ggccgttgct
     1381 tgccgtcgca accgaaaagc cggctgcgtc gagtgccgtc gacagtcgct cgagcacgcg
     1441 cagctcggaa tcagcgacga ccatgtggaa ctgctgcggc tgcttcgtgc gcgcacggcg
     1501 catcaggttt tcgatcgcga ccgcgaacgc gacatcgacg ggctcgtgaa actgaagacc
     1561 gatgccgggc acgcggcaca acgcgcgcgc ttcgtcggac gtgatggtgt gcatcacgcg
     1621 cgcagggttc gtgacgcgcg tcccgttgtg ctcgagcgaa acgagaactg gtgttccgac
     1681 cagaatcggt gacgtcgcga tgaacatgcc ggtgcgcgag atgtcttgca tcgcggcatg
     1741 ccgccactcg cgtgacgcgt aaaggtccaa actgagatcg agcgcgtatc gctctgactg
     1801 cctcggcacg atgtttcttc gtacagtgct tccgcccgcc cgcccaccat ttcgcgcggt
     1861 tgcatcccca gcgttaaccc gcggcactca cgcttctact tcacccagcg ctacccgatc
     1921 ctttgaagcg ctcgcgaaag tacgagccga tcgccgggcc gagcttgggc aacgcctgaa
     1981 gctgcgcgcc aagatacgag tgtccattct ggatcgcagt ctggactgcc tgatcgtggg
     2041 ccgcgcactc gggcgtgcgc acgtcgccaa ggatcttcca gctcgagcca ggtccgcagc
     2101 ggccaaatga cggatccgcg ccgccgatca cggccatcag atcgctcggt gccacttccg
     2161 tcatggcgga atcattgatc acttcgcatg ccagaccggt gaacgcgcat ctacacaggc
     2221 ttgtgtagta gccatgagcc catgggtatg cgggttgagg ccaaaagttt gcgcccaacg
     2281 gggagctggt cgtttattga gaacatggaa tccgccatga tcgatctggt tcgcgagctg
     2341 gacgacccga cgcgtcgccg cgatccagag ctcgaagtcg acatcgagat cgacgacatg
     2401 ctgctcgacc cgtggttcct gatgtccgag gacgagatcg cagagctcga cgatgatccg
     2461 aacgcgagcg tgctcgagat tcgtctcgcg ccgcgcgtcg accagctcga ctacgtcgcg
     2521 tagaccgccg tctagaagat cactgtgttg atcggcgcga cgttcatccc gccgggatag
     2581 ccgtacgaca cgttctgcgt gaacgtcgac gtcagcggcg ttccccagcc ccacacccat
     2641 agcccaaacg gcgcgtcgct cttgatctcg tggcgaccgt tgttgcactg accgaccgcg
     2701 gtgaagttgc cggtcgatag atcgacgcgc gtgtattcaa attcgccgcc gatgccggtc
     2761 caccccgaga gcacgcccgc gcaatcgagc tgcacgtcgt ggaagttgtt gtcggtgccg
     2821 cgcgagcgca cgacgacaag gttcgtctct ggatacgtcg gatcggtgaa gaacacgtac
     2881 tgccgcagat actgctcggg cggcacgctc gtcacgaagt ccggatcgcc gtagccggtc
     2941 gacaccgcag tcgagctcgc catgtacgtg aacaacatga atgggtgctc ggagtcctga
     3001 ctctggaccg tgaacggaat gccggtctgg aacgtaacct cttggcccgc gttgatcgtc
     3061 gtcgggccac caacggacgt cgagtacgtg agcgtcgtgc cgtcgacggc gccgaccatg
     3121 cgccagtacg tcgacgtctc gctcggcacg cgcggcttgt acatcacgcc gacatagcgg
     3181 ctgccgagtg ctttgaccgg cggcaccatc tgctcgccgt gatcgcagta cgcggtgccg
     3241 accggcactt gcatgcacgt atgaccggcc atgaagccga ctggcttgtt cgcctgcacg
     3301 acactgccgg tgagctcggc gggctgcgtg atctgcgcgt tctgaccctt gttgagcgag
     3361 atcatgatcg gctgaccggc ggcggcgccg ggaatgccgg tgcccgcggt gaccgcttgc
     3421 ttcggggtga gcatcacctg cgtgccgtct tcccgcgcga cgatgttgag cgaggggtgc
     3481 cccaccgacg gtgcttcggg cgacacgttg acggcgacgt agttcatatc ccagacgctc
     3541 gtcggtagca gcagcgatgc ggcggtgacg gcgacggagc cgccgccgta cggattgatc
     3601 tggtacgcga cgaccggtac gtcggtcttg atgtggaatg aatcgccgat tccggtgccc
     3661 gtgaagtacg ccgacggcac cgcgggcgtg accgggcagt ttggcgccga gccttgcgag
     3721 ccggcgagga acaggatcgc gacctcgccg ggtgcgaggc cttgcgtcgc atcgtaggtg
     3781 ccgtacgtga gcgacgagcc tgcgcccgtc ggcaagcgcg tgaacgtttg cgtgttgagc
     3841 ggctggccgc ggtactcgac atcgatgtgc gcggggaacg tccacgtgtt cgcgacgaac
     3901 accgcgtagc aatagccggg cgtcagcgtc tccatctcgg tcgcgaagta atcgcagccg
     3961 acggagcgat gattcgcttc ggctgccgcg cacgcgtcct gacacgtgta cgtcgacgga
     4021 tcgcacgctt ggccgccggt gcagtcttcg accacgctgc cgttacagtc gatcaccgaa
     4081 tggagatctg ctgagcaatt tcgcgaacac gatgcatcgg gctgtcccgt gtcgtcgtcg
     4141 tcgtcattgt gacgagcacc tggtccgcac gcgatcacaa acacggccat caacaacgct
     4201 gccttcatcg gtcacccccg gcggcgtgaa ccgtaatcgc agcgccacta accgatcaac
     4261 gggaatctgg tggggacaat tgtctccggc ggcgggctgt tacgaaatcg ttcttgacgg
     4321 ttcttgccac cggcctcaag gtggcgcgct tacgggagga gggaacggta catgcctacg
     4381 catcacattt tcgggtcaaa gggcggcaag gaggacgtgc gatgggaatc gccaagtccg
     4441 tcctgccgac gtatttcgac gtagccatca tcggcgcggg tccggtcgga ctcgcgatcg
     4501 cgatcgagct ggcgcgcctt gacctcacga cgctcgtcgt cgaccgccgg ccaccgccgg
     4561 ccgaggacac tggtctgcgc ccgcagctgc tcgtcgcccg tgccggtgac ctcgcgaacc
     4621 tcgcgcacct cggtgtcgat gttcgcgatc cccgcatcgt gacgctgctc gcatcgcgtt
     4681 gcgaggccga cctcgccacg ggtcgtacga tccgcggcgg cgtcgcgaca ctgcatagcg
     4741 tgccgccgca gctcgacctg tggacgctcg ctgcgcagcc gccgcgtgct ctggttccga
     4801 tcggtcgtct ccagcaggcg ctgctcgagc gtgctctgca ccatggtgcc tccgtgcact
     4861 atggctgcga ggtcacgcgc gtgcgccgcc atgcgcgctt tgcgtcgctg acgctcgcgg
     4921 atggtgtggc ggtgcgtgct gcgctggtcg tgatcgcgac cggtgctgcg cgctcgctgg
     4981 cgctggagct gctgcggggt gcgtgcgcgg tcgagctgcc ggagcagcgc atcatcggtg
     5041 cgctgttcga cgccgccgcc gacgagggtc gttggatccg cgtcgagctg ccggtgccgg
     5101 gtctcccgcg cctcgcgcgc gccaccgttc tgcagacgac cgacgcgggt accggtgtgc
     5161 tcgtttcggc accgggtcag ccgacggcgg agcagctcgg ccgctgcttc attacagcgg
     5221 cacgcgcgca tggcctgctc ggtgcgccga tgcttgtcga gccgcagatc ttcgcgaccg
     5281 cggttacggc gatggcacgg cgcaccattg cgggtgacgg ccgcgcgccg atcgtgatcg
     5341 caggtgatgc agcacagacc ggccacgtgt tcacgggcca gacgtgcttc gtcaacgtcg
     5401 cgctggcgct gcgcatggca cgcgagctac agcatgcacg tcgtgcaatt gccctgcagc
     5461 tagcgggcga tgcggcgctg ttgcgtgcgc tcgatcggta cgagcagcag tcgcagatcg
     5521 gtgcggcgat cctcgcgcgg acgtcgcagc gtcactatgc gccgcaccgg agcggccagt
     5581 gggctctcgc aggagttgcg cgtgctaatg ggttggcccg tcgagcgttt cgtctcggcg
     5641 ggccgtttgt ttttcggctt ccactccgcg ctataaagga ccaatggcca gttacgaaaa
     5701 gcgcacgtgg tggacgcgcc acggcctgac cgaccgactc gcgagcgaca cctttctcga
     5761 agcggcggtg acgagcgcgt ttttgatcgc agccgccgac ggcaacgcga gcgagcagga
     5821 gtacgacgcc ttgctcgatc gcctcgagat cctcggcggc gtcgatcgcg acaagatcga
     5881 cgagctgctc acgagcgtct cgaacgacat cgaggcgaac ggcttcgagg gccgcatcgc
     5941 gcgcgtcgcg gagatcgtcg gcgatcgcag cgcggcggaa gcggcgctga tgctcggcct
     6001 ggcgatcggt ctcgccgaca atgacttctc ggccgaggag cgcgaggtcg cgaaccaact
     6061 cgcaacggcg atcggtgtag gcgccgacga catcgaccaa cgagtcgccg agatccgcgg
     6121 ttagacgtag atgtaggtgc cagtcgctcc agcagcggtt tcgacatggc tggatgcggc
     6181 attcaccgct gctcacgtga tcttcacgca atagctcaga tcggcagagg tcgaaccatc
     6241 ggcaagaacc ttgctgatgc tcctcggcat gcggatcgcc atcgcctccc tccttctcgt
     6301 tggttgtgtt gctgcccagg acccgtcggg ctccgatgac gacgataggg ggtcgggctc
     6361 gggcagcggg tcggacatgg gtactggctc gggctcgggc tcgggctcgg gttccggttc
     6421 gggctcggcg cagccgccgg tcccggtggc gagtggcgcg taccaggtgc gcagcgacat
     6481 cgacctgacg gtcgaggccc tcttgccgga gccggtcgag aacatcgtgc tcacgctgcg
     6541 cgacttctcg atgaaccctg ccgagacgct gttcgacctc gcggaggaca tcggtgtgcc
     6601 ggccgtcggc gagattcgca ccgcgctgcc cgattacctc gaggacaagg ccctgggctg
     6661 gctcaacacc gagatcaaca aggtgacggt cggcggcgtg ccggtcccgc aggtcgcggg
     6721 caacatcgcg gcgctcgccg agaccgcgct gacgaagttc gcgctcgata gcgagctgac
     6781 gatcacggga cagaccgcga cgcatacgct gaagacgctc gacctcacgc cggctggcat
     6841 caacgtgcag gtcgccctcg aggcgctgcc gaccgagatc atcgcggcga cgccgtcgtg
     6901 ctcgtcgagc atgggtacgg ttgcgctcgg tgaccacagc tacagcctcc agtacggcga
     6961 gtacatgtgg cgcgcgatca acctcgcggt caccgcgcag tacggcaccg atgtccacgg
     7021 tgtgatcgcg gatgcagtca actgccaggc gatcgcgcac accgtcgcgt cgaagtgcta
     7081 cctcggcgtg tgcgtcggcc acgagtcgca gctgaccgac atctgcgaag cgggtgtgaa
     7141 cgaagtcgtc gagcgcgtgc acgacaagtt ctcggagatg cgcttcgacg cgattcactt
     7201 cgcggcgggt accgcggcgc tcgtcgatgc gaaccatgac ggcgatgccg aggcgatgac
     7261 gatgggcgtg tggatggccg agatcaatgc aggcctcggt ctgcgccacg tgccggcgac
     7321 gttcaccgcg acgaagtagt cgcgagcgcg aactggaccg tgccgccgag ccggatcgca
     7381 tgaaagcgac cgagctcggc gtttgcgatc acggcgacga ttggatagcc gccggtcgtc
     7441 ggatgttcgg ggccgagcac gatcggcacg ccatcgcccg gcacctcgag tgcacccatc
     7501 accatcggcc gcgactgctc gcgataggtc gccagtcgag gtaatgcggc gccttcgagc
     7561 cgggtgccga cgcgatcgct gctcggcgag atgcgatacg gagtaccgac gagctgaccg
     7621 agcacgttgt ctgcgaacgc atcgagatcg gggcccggca gcacctggat gacatcggtt
     7681 gcggtgaacg atgcgagcgc atgtgcgtcg gttgcgaggt gctcggcggg tgagagcgta
     7741 gcgcctgcgc gcaacggcgc accgagacga gcgcagagca gcgtgccgcg gccgtcgaga
     7801 atgcgcggtg cggcgatgcc tccagcgatc gcgagatacg tgcagcgcag cggttcgctc
     7861 tcgatcgtca atgtatcgcc ggcggcaagc agctgcggcg acgtgcgatc ggtcgcgacc
     7921 atgatctcgg cggtcgcgtt cacgatcagc tggccgagca cctcgatcgc cggtgcatcg
     7981 tccgcattcc cgacgctgcg gttcgcggca acgagcaact cgcgaacgag cgcgccgcct
     8041 ggtggcaccg cttcgtgcat gtgaccgcgg cgcccgaggt cttggaccgt gcacagaccg
     8101 aacgcgcggc tgacgacgag gctcatgaca cgaaccgcac gcgatcgccg agctgaagca
     8161 gcgccccccg ctcgtcgaac atcgtcgtct cgacgacgcg accgatcagg tgccagccgc
     8221 ccggcgagtc gaacgggtag accgcggtga atgcatccgc gatcgcgagc gagccggcgg
     8281 gtacgcgtgg gcgcggcgtc gcgcg
//
LOCUS       DASUFX010000032         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1811741, whole genome shotgun sequence.
ACCESSION   DASUFX010000032 DASUFX010000000
VERSION     DASUFX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811741"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFV99_01290"
     CDS             <1..1036
                     /locus_tag="VFV99_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057955.1"
                     /translation="KFYVWTPAQLREVLGSVNTLVFAQAYGVTDQGNFEHQTTVLSRI
                     TPRTSPHEEEQLAELRAKLFAARGQRVRPGTDDKLLAAWNGLAISGLVAAWRATQHAP
                     ALTLALRVASFLREKMIKNGRVARVFHQGHTKDLDGTLDDYAFVAQGFLELAEATGDR
                     LWWDLATALLKTVRADFVEERDGVVIFYMSPAGDALLVHRPESNHDGAIPSGAAIATQ
                     ALLRIGLVAGDADALALAEKYLAQRLTAVTGANAWATSALLGALDLYLHAKELVVTDG
                     EGRAELLAAARRSYSPTLCIAGPWAQATILDGKTADGARARAFVCSGPTCAPPVTEPT
                     ELVKLVSSTG"
     gene            complement(1020..>2009)
                     /locus_tag="VFV99_01295"
     CDS             complement(1020..>2009)
                     /locus_tag="VFV99_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057956.1"
                     /translation="NLANSGTSAVGATAMKAVPFTVNYAANTGTDHYEVHTRCGTVNV
                     GLLTAAQIALPIGCTATPMNIEVIARSSAGVALAWTQQPNVAITSGGSTTIADTWHAP
                     VQLAATYTNPTPQVSDITVARFSPYVRGLPVAEVMSATSATTNVMLNVSQTPSASLST
                     SLLCPMSATPGCLATPSGTASQRITQAVDGTLATYALDIGANLLPWVKALYVPATTSL
                     DITVTGSGAFDIFEANLQYTRGQTIYVWRVFGPLAQSVTFPTLPATAPGDPTVRTTDV
                     QSSYQAFVGESDAINGYRDAIKNPFEALGTCDATSSTTAKPYPGTTARISQWN"
BASE COUNT          309 a          641 c          704 g          355 t
ORIGIN      
        1 aaagttctac gtgtggacgc cggcgcagct gcgcgaggtg ctcggctcgg tcaacacgct
       61 cgtgttcgcg caggcctatg gtgtgaccga ccagggcaac ttcgaacatc aaacgaccgt
      121 gctgtcgcgg atcacgccgc gcacctcgcc gcacgaagaa gagcaactgg cggagctgcg
      181 cgcgaagctg ttcgcggcgc gtgggcagcg cgtgcgtcct ggcaccgacg acaagctgct
      241 tgccgcgtgg aacggtctcg cgatcagtgg gctcgtcgcc gcgtggcgcg cgacccagca
      301 cgcaccggcg ctgacactcg cgctgcgggt cgcatcgttc ttgcgcgaga agatgatcaa
      361 gaacggacgt gtcgcacgcg tgttccatca gggccacacg aaggatctcg acggtacgct
      421 ggacgactac gcgttcgtcg cgcaggggtt cctcgagctc gctgaagcga cgggcgatcg
      481 gctgtggtgg gacctcgcca cggcactgct gaagaccgtg cgcgccgact tcgtggaaga
      541 gcgcgacggc gtcgtcatct tctacatgtc gccggccggt gatgcgctgc tcgtgcaccg
      601 tcccgagtcg aaccacgacg gcgcgattcc atcaggtgct gcgatcgcga cgcaggcatt
      661 gctgcggatc ggccttgtcg caggcgatgc cgatgcgctc gcgcttgccg agaagtacct
      721 cgcgcagcga ctcaccgcgg tgaccggcgc caacgcgtgg gcgacatcgg cgttgctcgg
      781 cgcgctcgat ctgtacctgc acgcgaagga gctggtcgtc accgatggcg agggccgtgc
      841 agagctgctc gcggccgcgc gccggagtta cagcccgacg ctgtgcatcg ccggcccgtg
      901 ggcgcaggcg acgatcctcg atggcaagac cgccgatggt gcgcgcgctc gtgcgttcgt
      961 gtgcagcggc ccgacgtgtg caccgccggt gaccgagccg accgaattgg tcaagctcgt
     1021 cagttccact ggctgatgcg ggccgtcgtg ccgggatacg gcttcgcggt cgtgctcgaa
     1081 gtcgcgtcgc aggtgccgag cgcttcgaat ggattcttga tcgcgtcgcg atagccgttg
     1141 atcgcatccg attcgccgac gaaggcctgg tagctagact gcacgtcggt cgtgcgcacg
     1201 gtcggatcgc cgggtgcagt tgccggcagc gtcgggaatg tcacgctctg tgcgagcggg
     1261 ccgaatacgc gccacacgta gatggtctgg ccgcgcgtgt actggaggtt cgcttcgaag
     1321 atgtcgaacg cgccgctgcc ggtcaccgtg atgtcgagcg atgttgtcgc cggcacgtac
     1381 agcgctttga cccacggcaa gaggttcgcg ccgatgtcga gcgcataggt ggcgagcgtg
     1441 ccgtcgaccg cttgtgtgat gcgctggctt gccgtaccgc tcggcgtggc gaggcagccg
     1501 ggcgtcgcac tcatcgggca gagcagggag gtcgacagcg atgcgctcgg cgtctgcgac
     1561 acgttcagca tgacattcgt cgtggcactc gtcgcgctca tcacctcggc gacgggcaga
     1621 ccgcgcacgt acggcgagaa ccgcgcgacc gtgatgtcgc tgacctgcgg cgtcggattc
     1681 gtgtaggtgg ctgcgagctg caccggcgca tgccaggtgt cggcaattgt cgtcgagccg
     1741 cccgacgtga tggcgacgtt cggctgctgc gtccatgcga gcgcgacacc cgcgctcgac
     1801 cgcgcgatga cttcgatgtt catcggcgtc gccgtgcagc cgatcggcag cgcgatctgc
     1861 gctgctgtca agagcccgac attgacggtg ccgcagcgcg tgtgcacctc gtaatggtcg
     1921 gtgccggtgt tcgccgcgta attgaccgtg aacggcaccg ctttcatcgc cgtcgcgccg
     1981 accgcactcg tacccgaatt ggcgagatt
//
LOCUS       DASUFX010000033         8691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_561327,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000033 DASUFX010000000
VERSION     DASUFX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561327"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFV99_01300"
     CDS             complement(<1..907)
                     /locus_tag="VFV99_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057957.1"
                     /translation="MRWCLLLYAMTACMRVESTPGGGTEHQITLTIDRRATVGDVSSL
                     LISISSAGTTTTDTVVASEFPLFHTVTADGLIGRVDIGVDALDDAGHLVGRGEATTDA
                     NAGRASVMMQGADFLLNSSYTGDQLLTNDFDAAGVQLAATSAGAWTVAFRDECTSCGI
                     YARRFDASGFPLASGSSGSTAQLTMSSVPTRPDANPAVAAGATDTLVVWDFTDLSTPD
                     TQGVACHPLDAEGSEPAPGQQTIASEISDVVTAVALASGDFVVTWNGFMTTSTIRSMI
                     VRPDCSAVTSPTTISTPAGVKGPYRA"
     gene            complement(1026..1514)
                     /locus_tag="VFV99_01305"
     CDS             complement(1026..1514)
                     /locus_tag="VFV99_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057958.1"
                     /translation="MRWVVVLCALAGCSRVTTTPGGDTGGSTITLMVSKRIPVHDVAS
                     LVVTITNGGSTTSETVQVTDQSFPLNYDANAPGMTGSLDIKVEAFDAGRFLVGRGTTT
                     AELGVEGAAVMLQGVDFTLNSVYAGNQFLTNDFETVGLRRCLRGADEQRGRSDESVRN
                     HA"
     gene            complement(1555..3684)
                     /locus_tag="VFV99_01310"
     CDS             complement(1555..3684)
                     /locus_tag="VFV99_01310"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5057959.1"
                     /translation="MSKRFRAGMVGAGNICEFHVAAVKALPDVELIGITDLDRKRAHE
                     NAEKWGTTAFDSLDQLIDAGANVIHVLTPPAAHATVALAALNRGCHALVEKPLAEDEG
                     DAVAIGRVANAKGLVASVNHSLLYDPQIKRALDMVRSGALGEVVSVDILRGSEYPPYE
                     GGPLPPWYRDAGYPFRDIGVHCLYLIQQLLGPIEDVDAEWRSLGGDPNLAFDEWRALV
                     RCKRGLGQFQLTYNVKPMQSQLIIHGTKGVLRVDLFAMFHAKRGSTPLPKAAERLINA
                     FTDSLQPLVDVPVGVYKFVTKQVQPYQGLRDLVRDFYQRLENGEPPPVSITDAATVVR
                     WVEKVAREADDDHKNRLAKFPVSETVPFLVTGASGSVGRAVVKRLRDTGHRVRVFVRR
                     TPDKPQDGVEYMFGNLGDPAAVDRAVRGIETVIHCGAAMKGGWPEHKGGTVAGTQNVI
                     DACKKHGVKQLVHISSMSVVDWAGSVGAAPVSETANLEPRPDERGAYTRAKLEAERLV
                     TDAAANGLPCVILRPGQIFGGGIPLINGAVARNAGGRWLVLGDGRLELPLVYIDDVVD
                     AIINAVDKRLTKGDVIQIIDPEHLTQQDVLELAGGDKPILRVPRPIVFALGKLSELPL
                     GLLGKQSPVAVYRLKSALARLHYESERARVLLDWKPRIGVREGIRRVTNTPVVAPQPT
                     EPKQASNGTKPSELRPLVVRDVGNARI"
     gene            complement(3681..4841)
                     /locus_tag="VFV99_01315"
     CDS             complement(3681..4841)
                     /locus_tag="VFV99_01315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5057960.1"
                     /translation="MSTPIIAFAKDWHEDPTSNHHVLRELAKTRRVLWLNSIATRTPN
                     LSSTRDLGKIARKVREFARGPVNVENDLWVVTPLAVPLPGSPTARAINRQILRAQIAT
                     LRLRMGIARFQLWTFLPNTADYVGTLGEDCSIYYCVDEWSLFSYLDRAQTIAAEKALL
                     EKVDAVFAINGPLAEAKRALNPCTYVSPHGVDHALFAQALDEALAVPAELAALPRPII
                     GFYGTLRDWVDFELIAHVARNRPDWSVVLIGQQFADMTPIQGLANVHLLGQKPHRELP
                     AYCKAFDVGMIPYRIDERMTFVNPLKLREYLSAGLPVVSTPVPEVTRLSHLAHVAATK
                     EDFVSAVDRALSESSPAHRGERSHAMKNETWAARVAQVARTVDEISERKQLR"
     gene            4992..6446
                     /locus_tag="VFV99_01320"
     CDS             4992..6446
                     /locus_tag="VFV99_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057961.1"
                     /translation="MSEALRLRDGDPLPKTSPFYDGERIRLVAARGETIAFQVVTRTF
                     ADSSLIIKANGVSVRRWATLALQAKRASTSMYGATLGTGWYRDRLEPTDSLDDEAIRL
                     LETKGTLFELVVARDADVGMFTGNVIVDQKWIPLDLTIVDVTLPPIPPRVWAYYDPRE
                     LEWAKLGTGSIEAPSEQEKRCIAMFRDYGVVLSVDMTPAAYPARKELLGDFPYVPVRL
                     PKNVSAVAADVRAWIDATKGTGKLPFAIPIDEPRTPDARAKVKELATVVRAAGGGPST
                     FLYAVTAEPHPDFADLVDLYITLKPRRTDTLPRWTYNGAPPRAGSMVLDTVSPGTRTW
                     GWIGYRYNIPVWYVWDALYWNDRHNRKGAPLPGREFGVYDAISFDDGEDRGNLDGVLA
                     LPGDTTMPCRPTLRLAAIHRGMQDRQLIELAAQCKPDETDKLVAQMVPRALGDIPGRT
                     VAGDGSPSWPTDDAAWESARQKLLEIAGRCVTPR"
     gene            complement(6433..7347)
                     /locus_tag="VFV99_01325"
     CDS             complement(6433..7347)
                     /locus_tag="VFV99_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057962.1"
                     /translation="MTKRPSENLPVLAPQRLGAPLPMVTQPIQAIVDDPLVTRADNPL
                     SRIDPALMPMHARAGEAVVFSNAVPTKALATMRPAPVGPPPEIGLTQHALPPDEPYDP
                     RLALVIDPDSERSAAFRVLRHHLLGLGRPQVIIVSSPLKGEGKTTTALNLALALAECG
                     RSKVLLAETHVRRPQLANVCKLVPPWCFAEQLAAHRHQPMLPWSLVEIPQLWLHVAAI
                     NPRIEKTQLLDAPAFAIAMERLRLGGYDHIVIDAPPVLGSADVNLMADSADAVVFALR
                     ARQSNTRDVRRAMDQIGGNRVVGTVIVE"
     gene            complement(7344..>8691)
                     /locus_tag="VFV99_01330"
     CDS             complement(7344..>8691)
                     /locus_tag="VFV99_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057963.1"
                     /translation="YQERIRSSLLQGREEQVQRGIGDRYRELLLARGQLAQVIADPKL
                     NPFPDESDTDLAIDKLREKIRFESRGANAFRITYSDGDPERAKNVTEKLTTLLQDKDE
                     FLRRDQARVTVAFAIKQKDEAAAELAKAETSLAEFLAKHPEFAQESNAPAGQGAMIGE
                     GAAIRGAQNQKSTAKNENARLYALERQRQRIQARLDAPPDAPPIRVPAPVTPEKIAAE
                     NAVTEARSRLASANRELDSALDKYTDQHPNVMRAKSRVADAQAELKRAQAAVPPDVET
                     TIAPATEADRTKLKEELSRLDSQIGEEQRRGKNAPSVADVTTNWVVQLETEHAKLRRA
                     VTEDRERVEALSDSVFRAQMDANQKLADTGAVLSVVDPAFRPVKPSGPGKTLILLAGM
                     ALFVALGSGLALGMAVIDDRVYRRTDLEELGIAVLAVIPPGPTKAPRPTKRKAARK"
BASE COUNT         1384 a         2873 c         2927 g         1507 t
ORIGIN      
        1 gcgctcggta ggggcccttg acgcccgctg gcgtcgagat cgtcgtcggc gaagtgaccg
       61 ccgagcagtc ggggcggacg atcatcgagc ggatggtgct cgtcgtcatg aacccgttcc
      121 acgtcacgac gaagtcgccg ctcgcgagcg cgaccgcggt cacgacgtcc gaaatctctg
      181 atgcgatcgt ttgctgcccg ggcgccggct cgctgccctc tgcgtcgagc ggatggcacg
      241 cgacgccttg cgtgtcgggc gtgctcaggt cggtgaagtc ccagacgacg agcgtatctg
      301 tcgcgccagc cgccacagcg gggtttgcat cgggccgcgt cggcacgctg gacatggtga
      361 gctgtgcggt gctccccgag ctgcccgatg cgagcggaaa cccagacgcg tcgaagcgcc
      421 gggcgtagat tccacagctc gtgcactcgt cacggaatgc gacggtccac gcgcctgcgc
      481 tcgtcgcggc aagctgaaca cccgcggcgt cgaagtcgtt cgtcaacagc tggtcgccgg
      541 tgtaggagct gttgagcagg aagtcggcgc cttgcatcat cacgctcgcg cgccccgcgt
      601 tggcatctgt cgtcgcctcg ccgcggccga cgaggtgtcc cgcgtcgtcg agagcgtcga
      661 cgccaatgtc cacgcgaccg atcaagccgt ccgcggtgac cgtgtggaac aacggaaact
      721 ccgaggccac cacggtatcg gtggtcgtcg ttcctgcgct ggagatcgag atcagtagcg
      781 agctgacgtc gcccacggtc gcgcgccgat cgatcgtgag cgtgatctgg tgctccgtgc
      841 cgccgccggg cgtgctctcc acgcgcatgc acgcggtcat cgcgtacagc aggagacacc
      901 agcgcatgcc ggcatcctac aactcgattc acagatctgg gacgaggatc cgtgcgcgta
      961 cggccgtgcc ggaggtgtca gggtcggtcg cgctcgcgtc ggtccacgcg acgatgaacg
     1021 agtcgtcaag cgtggttacg gacggattcg tctgatcgcc cgcgctgttc gtcggcacca
     1081 cgaagacatc gccgcagccc gacggtctcg aagtcgttcg tcaggaattg gttgccggcg
     1141 tagacgctat tgagcgtgaa gtccacgccc tgcagcatca cggcggcgcc ctcgacgccg
     1201 agctcggccg tcgtggtgcc gcggccgact aaaaacctgc ccgcatcgaa cgcttcgacc
     1261 ttgatgtcga gggagccggt catgccgggc gcgttcgcgt cgtagttcag cgggaacgac
     1321 tggtcggtca cctggacggt ctcgctggtc gtcgagccgc cattcgtgat cgtgaccacg
     1381 agcgacgcga cgtcgtgcac cgggatccgc ttcgagacca tcagggtgat cgtcgacccg
     1441 ccggtgtcac cgccgggcgt ggtcgtgacg cgcgagcagc ccgcaagcgc gcacagcacg
     1501 acgacccacc gcatgggctc atcgtacagc acgcgcagcg ccggacgggc cgcttcagat
     1561 gcgcgcgttg cctacgtcac gtacgacgag cggccgcagc tcgctgggct tggtgccgtt
     1621 gcttgcctgc ttgggctcgg tgggctgtgg cgcgacgacg ggtgtgttcg tgacgcgacg
     1681 aatgccttcg cgcacgccga tccgcggctt ccaatcgagc agcacgcggg cgcgttcgct
     1741 ctcgtagtga aggcgcgcga gcgccgattt caggcgatac accgcgaccg ggctctgctt
     1801 gccgagtaag ccgagcggaa gctcggacag cttgccgagc gcgaacacga tcggacgcgg
     1861 tacgcgcagg atcggcttgt cgccgccggc gagctcgagc acgtcctgtt gcgtgaggtg
     1921 ctcggggtcg atgatctgga tgacgtcgcc cttggtcagg cgcttgtcga ccgcgttgat
     1981 gatcgcgtcg acgacatcat cgatgtagac gaggggcagc tcgagcctgc cgtcgccgag
     2041 cacgagccag cgaccacccg cgttgcgcgc gactgcgccg ttgatcagcg gaatgccacc
     2101 gccgaagatc tggcccgggc gcaggatcac gcagggcagt ccgttcgcgg ccgcgtccgt
     2161 caccagccgt tcggcttcga gcttcgcgcg cgtgtacgca ccacgctcgt caggacgcgg
     2221 ctcgaggttc gctgtctcgg agaccggcgc tgcgccgacc gacccggccc agtcgacgac
     2281 cgacatcgac gagatatgaa cgagctgttt gaccccgtgc ttcttgcacg catcgatcac
     2341 gttctgcgtg ccggcgacgg tgccgccctt gtgctcgggc caaccaccct tcatcgcagc
     2401 gccgcagtgg atcacggtct cgatgccacg caccgcgcgg tcgaccgcgg ccggatcgcc
     2461 gagattgccg aacatgtact cgacaccatc ctgcggcttg tccggcgtac ggcgaacgaa
     2521 cactcgcacg cgatgaccag tgtcgcgcag ccgcttgacg accgcgcggc cgaccgaccc
     2581 cgatgcgccg gtaacaagga acggcactgt ctcggagacc gggaacttcg cgagccggtt
     2641 cttgtgatcg tcgtcggctt cgcgcgcgac cttctcgacc cagcgcacga ccgtcgcagc
     2701 gtctgtgatc gagaccggcg gtggctcacc gttctcgagg cgctgataga agtcgcgcac
     2761 gaggtcgcgc aggccctgat agggctggac ctgcttcgtc acgaacttgt agacgccgac
     2821 tgggacgtcg acgagcggct gcagcgaatc ggtgaacgcg ttgatcagcc gttcggctgc
     2881 tttcggcagc ggcgtcgagc cgcgcttcgc gtggaacatc gcgaacaggt cgacgcgcag
     2941 cacgcccttc gtgccgtgaa tgatcagctg cgattgcatc ggcttcacgt tgtacgtgag
     3001 ctggaattga ccgaggccac gcttgcagcg cacgagcgcg cgccactcgt cgaatgcgag
     3061 attcggatcg ccgccgagcg agcgccactc cgcatcgaca tcctcgatgg ggccgagcag
     3121 ctgctggatc aagtacaagc agtgcacgcc gatgtcgcgg aacggatagc cggcatcgcg
     3181 ataccacggt ggcagcgggc cgccttcgta cggcggatat tccgagccac gcaggatgtc
     3241 gaccgacacg acctcgccca gcgcgccgga tcgcaccatg tcgagcgcgc gcttgatctg
     3301 tggatcgtag agcagcgaat ggttgacgct cgcgaccagc cccttcgcgt ttgcgactct
     3361 gccgatcgcg actgcgtcgc cttcgtcctc cgcgagcggt ttctcgacga gcgcatggca
     3421 gccgcgattg agcgcggcga gcgcgaccgt tgcgtgggcg gctggcggcg tgagcacgtg
     3481 gatcacattt gcgccggcgt cgatcaactg atcgagcgag tcgaatgcag tcgtgcccca
     3541 cttctcggcg ttttcgtgcg cgcgtttgcg atcgagatcg gtgataccga tgagctcgac
     3601 atcgggcagc gccttcacgg cggcgacgtg gaactcgcag atgttgcctg cgccgaccat
     3661 gccggcgcga aaccgtttgc tcatcgtagt tgtttcctct cgctgatctc gtccacggtg
     3721 cgtgccacct gcgcgacgcg tgccgcccac gtctcgttct tcatcgcgtg cgatcgctca
     3781 ccgcgatggg ccggtgagct ctcggaaagc gcgcgatcga cggccgagac gaagtcctcc
     3841 ttcgtcgccg caacatgcgc gaggtgcgat aggcgcgtga cctcgggcac cggcgtcgag
     3901 acaacgggca agcccgccga caagtactcg cgcaacttga gcggattgac gaaagtcata
     3961 cgctcgtcga ttcggtaggg aatcatgccg acatcgaagg ctttgcaata agcgggcaac
     4021 tcgcgatgcg gtttctgtcc gagcagatgc acgttcgcaa gaccctggat tggtgtcatg
     4081 tctgcgaact gctgaccgat gagcaccacc gaccagtcgg gacgattccg cgcgacgtgc
     4141 gcgatcagct cgaagtcgac ccagtcgcgc aacgtgccat agaagccgat gatggggcgg
     4201 ggcagggccg cgagctccgc cggcaccgcg agcgcctcgt ccaacgcctg cgcgaacagg
     4261 gcgtgatcga cgccgtgcgg cgagacatac gtgcaagggt tgagcgcgcg cttcgcctcg
     4321 gccagcgggc cattgatcgc gaacaccgca tcgaccttct cgagcagcgc cttctcggcc
     4381 gcgatggtct gcgcgcgatc gagatagctg aacagcgacc attcgtcgac gcagtaatag
     4441 atggagcagt cctcgccgag cgtaccgacg tagtccgccg tgttcggcag aaacgtccac
     4501 agctgaaacc gcgcgatccc catgcgcagc cgcagcgtcg cgatctgcgc gcgcaggatc
     4561 tggcggttga tcgcgcgtgc cgtcggtgag cctggcagcg gcaccgcaag cggcgtcacg
     4621 acccacaaat cgttctcgac gttcaccggc ccgcgcgcga actcgcgaac cttgcgcgcg
     4681 atcttaccga ggtcgcgcgt gctagacagg ttgggcgtgc gcgttgctat ggagttgagc
     4741 cacagcacgc ggcgcgtctt cgcaagctca cgcagtacgt gatggttcga agtcgggtcc
     4801 tcgtgccagt ccttcgcgaa tgcgatgatc ggagtgctca cgtcgacgga aaacggtaac
     4861 ggcgcatgac taggtcggca acaccagcta ccccagctca ctgtgccgtc acgtgcgcga
     4921 ttgccacgtg tgcgctcgcg ctcgcgtgca aaaagcaaac tcccgcggat cggggcccgg
     4981 atctgcaagt cgtgagtgag gcactccgcc tgcgtgacgg cgatccactc cccaagacct
     5041 caccgttcta cgatggcgag cgcatccggc tcgtggccgc gcgtggcgag acgattgcgt
     5101 tccaggtcgt cactcgcacg ttcgcggact cgtcgctcat catcaaagca aacggcgtct
     5161 cggtacgacg ctgggcgacg ttggcgctgc aagcgaagcg cgcttcgacg tccatgtacg
     5221 gggcaacgct aggcactggt tggtatcgcg accgcctcga gccgacggac tccctcgacg
     5281 atgaagcgat tcgcctgctc gagacgaagg gcacgctgtt cgagctcgtc gtcgcccgcg
     5341 atgcggacgt cggcatgttc accggtaacg tgatcgtcga tcagaagtgg attccgctcg
     5401 acttgacgat cgtcgacgtc acgttgccgc cgataccgcc gcgtgtgtgg gcttattacg
     5461 atccgcgcga gctcgaatgg gccaagctcg gtaccggctc gatcgaagca cccagcgagc
     5521 aagagaagcg atgtatcgcg atgttccgcg actacggtgt cgtgctctcg gtcgatatga
     5581 cgcccgccgc gtatcccgcg cgcaaagagc tactcggcga ctttccgtac gtgcccgttc
     5641 gcctgccgaa aaacgtttca gccgtcgctg ccgacgtccg cgcgtggatc gacgcgacga
     5701 aaggcaccgg caagctgccg ttcgcgattc cgatcgacga gccgcgcacg cccgacgcgc
     5761 gggccaaggt caaggagctc gcgaccgtgg ttcgtgcagc cggcggcggt ccgtcgacgt
     5821 ttctctacgc ggtcaccgcc gagccgcacc ccgacttcgc cgacctcgtc gatctctaca
     5881 tcacgctcaa gccaagacgc accgacacgt taccgcgctg gacgtacaac ggcgcgccgc
     5941 cgcgcgcggg ctcgatggtg ctcgacacgg tgtcgccagg cactcgtacg tggggctgga
     6001 tcgggtaccg ctacaacatc ccggtctggt acgtctggga tgcgctctac tggaacgacc
     6061 gtcacaaccg taagggcgcg ccgctccccg gtcgcgagtt cggcgtctac gacgcgatca
     6121 gtttcgacga tggcgaagat cgcgggaacc tcgacggcgt gctcgcgctt cccggcgaca
     6181 cgacgatgcc atgccgcccg acgctgcgcc tcgctgcgat ccatcgtggc atgcaagacc
     6241 gtcagctcat cgagctcgcg gcgcagtgca aacccgacga gacagacaag ctcgtcgcgc
     6301 aaatggtccc gcgcgcgctc ggcgacattc cgggacgaac ggtggcgggc gatggctcac
     6361 cgtcgtggcc gaccgacgac gcggcctggg aatccgcgcg ccagaagctc ctcgaaatcg
     6421 caggacgctg cgttactcca cgataacggt accgaccacg cgattgccgc cgatctgatc
     6481 catcgcgcgg cgcacatcac gcgtattcga ctggcgcgcg cgcaacgcga acacgaccgc
     6541 gtcggccgag tctgccatca ggttcacgtc cgccgagcca agtaccggcg gtgcgtcgat
     6601 cacgatgtgg tcgtagccgc cgagccgtag gcgctccatc gcgatcgcga acgcgggcgc
     6661 gtcgagcagc tgcgtcttct cgatgcgcgg attgatcgcg gcgacgtgca accacagctg
     6721 cgggatctcg acgaggctcc acggcagcat cggctgatga cggtgcgcgg cgagctgctc
     6781 tgcgaagcac cacggcggca cgagcttgca cacattcgcg agctgcggcc ggcgaacgtg
     6841 cgtctcggca agcagcacct tggagcgtcc gcactcggcg agcgcgagcg cgaggttcag
     6901 cgccgtcgtc gtcttgcctt cgcccttgag cgggctcgag acgatgatga cctggggacg
     6961 gcccaggccg agcaggtgat ggcgcagcac gcggaacgcg gccgagcgct ccgaatctgg
     7021 atcgatcacg agtgcgaggc gcgggtcgta cggctcgtcg ggcgggagcg catgctgggt
     7081 caggccgatt tccggcggcg ggccgaccgg cgccggcctc atcgtcgcca gtgccttcgt
     7141 cgggaccgca ttgctgaaca cgaccgcctc gccggcacgg gcgtgcattg gcatgagcgc
     7201 ggggtcgatc cgcgacagcg ggttgtcggc gcgcgtcacc agcgggtcgt cgacgatcgc
     7261 ctggatcggc tgcgtcacca tcggcagcgg cgcgccaagg cgctgtggcg cgagcacggg
     7321 cagattttcg gaggggcgct tcgtcatttg cgggcagcct ttcgcttcgt gggacgcggc
     7381 gccttcgtcg ggcccggcgg gatgaccgcc agcacggcga tgccgagctc ttcgaggtcg
     7441 gtgcggcgat agacgcgatc gtcgatcacc gccatgccga gtgcgaggcc gctgccgagc
     7501 gcgacgaaca gcgccatgcc tgcgagcagg atgagtgtct tgcccggacc gctcggcttg
     7561 acgggccgga acgccgggtc gacgacgctg agcacggcgc cggtgtcggc gagcttctgg
     7621 ttcgcatcca tttgcgcgcg gaacacgctg tcggacagcg cttcgacgcg ctcgcgatct
     7681 tcggtgaccg cgcggcgcag ctttgcgtgc tcggtctcga gctggacgac ccagttcgtc
     7741 gtcacgtcgg cgacgctcgg cgcgttcttg ccgcgccttt gctcttcgcc gatctgcgaa
     7801 tcgagacgcg acagctcttc cttcagcttg gtgcggtcgg cttcggtggc tggcgcgatc
     7861 gtcgtctcga cgtccggcgg caccgcggcc tgcgcacgct tgagctcggc ttgcgcatcg
     7921 gcgacgcggc tcttcgcgcg catcacgttc gggtgttgat cggtgtactt gtcgagcgct
     7981 gagtcgagct cgcgattcgc ggaagcgagc cgggagcgcg cctcggtgac cgcgttctcg
     8041 gcggcgatct tttcgggcgt caccggggca gggacgcgaa tcggtggcgc gtcgggcggc
     8101 gcgtcgaggc gcgcctggat gcgctggcgt tgacgctcga gcgcgtacag ccgggcgttc
     8161 tcgttcttgg ccgtcgactt ctggttctgc gcgccacgaa tcgcggcgcc ttctccgatc
     8221 atcgcgcctt ggcccgcggg cgcattcgat tcctgcgcga actcgggatg cttggcgagg
     8281 aactcggcga gcgacgtctc ggccttcgcg agctcggcgg cggcctcgtc cttttgcttg
     8341 atcgcgaacg cgaccgtgac gcgcgcctgg tccctgcgca ggaactcgtc cttgtcctgg
     8401 agcagcgtcg tcagcttctc ggtcacgttc ttcgcgcgct ctgggtcgcc gtcgctgtac
     8461 gtgatgcgga acgcgttggc gccgcgcgat tcgaagcgga tcttctcgcg cagcttgtcg
     8521 atcgcgagat cggtgtcgga ctcgtcgggg aacgggttca gcttcgggtc cgcgatgacc
     8581 tgcgcgagct ggccgcgtgc gagtagcagc tcgcgatagc gatcgccgat gccgcgctgt
     8641 acttgctcct cgcggccctg caggaggctc gaccggatgc gctcctgata g
//
LOCUS       DASUFX010000034         5179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1395274, whole genome shotgun sequence.
ACCESSION   DASUFX010000034 DASUFX010000000
VERSION     DASUFX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395274"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1029
                     /locus_tag="VFV99_01335"
     CDS             91..1029
                     /locus_tag="VFV99_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5057964.1"
                     /translation="MRVFVAIADDVFDTGFASLLDTFETANELAGESRFSVTVVSPRS
                     SVRTHHGLAIRPPRRPARGPDLVIVPALACKHPATIVRALDRPDVQALIDLVVRYARR
                     CRVAAACTGTFVLGRAGVLDGRRATTTWWLGPTFRDLFPAVELEEAEMLIADKGVLTA
                     GAALAHIDLALAVIRERSPELASLVARYLLIDRRPSQAPFVAPSQVACDDELVKRFER
                     WVRQHLGEPFELARAARAVGASERTLQRRIRTVLGRPPIAFVQDLRLERAAHLLRVTD
                     DSVDAIAGAVGYEDGSTLRTLLRRKMRVGVRELRAR"
     gene            complement(1063..1335)
                     /locus_tag="VFV99_01340"
     CDS             complement(1063..1335)
                     /locus_tag="VFV99_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057965.1"
                     /translation="MNKDAFSTIDSSELTHVCGGSFSFDLPGSSGDGGGARDQDLPPS
                     STKRSVKIGYKGIGFEGQREDTNTDYSLCLRKHPKDTKEQCGLPPA"
     gene            1712..2560
                     /locus_tag="VFV99_01345"
     CDS             1712..2560
                     /locus_tag="VFV99_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX5057966.1"
                     /translation="MSPIALSLVLTSCALTVDDRELEESPDASATVSEPEAEPWAIKT
                     LSPISGANQLQINNALVGGGVVRLLAGTYVISDTIRMTSNSTLRGEPGAIIKLVGDAD
                     WPKYKPLIQGQSVSNVRITGLTIDGNGSNNTDSNGVSTVCGKYFYTLMHFTSSKNVEV
                     DHSSLGNNWNDVLKLNSSSNIRFHHNKVRREGHDVVYAIKSASVHVYDNDIKIACNSG
                     VRADGTKPMSIYRNTITRDGGGYAGIEIQGTSEVWACNNNIYGLVGPTIWNISGAPVH
                     TSGCPQ"
     gene            complement(2621..>5179)
                     /locus_tag="VFV99_01350"
     CDS             complement(2621..>5179)
                     /locus_tag="VFV99_01350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5057967.1"
                     /translation="DVDTERDFIVMELVRGESLRAKLKRERVLSIQEIVRVGQSLLDA
                     LAAAHAAKIVHRDVKPANILIDEANTIKLVDFGIASFGDRDLTSTGVRIGTPAYMAPE
                     QLRGRVADVRADLYAVGATLFEAATGEQLHASSRAPDQVTAAVLAATGDAALASAITR
                     AVAEQPDDRFASAREFADALGSTRHLATDDAAKRPAKHADDVAKRPARRRWWLAGGAA
                     VIAAGALVVLARPRGHDMPVAARTAAAPADATHERPSIAVLPFIDHTGEARLDFAASG
                     LPHILATQLRSIPELHVLGYYELLDHLPSGSAPAEAWERAARSLGAETVIHGEIFTEQ
                     AGIRVVVKVTTINGGELGRFERMTSVDSVPAATRSIAADVARVAVGRAPQALGDARKF
                     EIDRNLQLAISAIERQDFTAADGYLDTVEREAPDLAEAQYYRALLNWWLSRPIDAAVT
                     RALAGNLESAQRGFMEGLKLVYDQSHLTPAINYFRELVTHFPDHREINYGLFEALYHG
                     GFPAEAMVVYRRLALRHPRFKLGLKHALSYYVGHADDDGMRWAIARLEPDLEESLLWQ
                     ARAHVARREYAAAIALLRRHDSKGATFEVRRELVEIYLLDGQFELAADVAADWSSADV
                     PQRASQLLALANAFGDVNAADAWSHKAAAAAELMTGSDERERGWLELAAIEMPDARRD
                     RLRPILPKLTTENINQVVAHTLVAGTLDERTLVDAARKSEFPEVVAVANALHAEATKN
                     LNGAADYWKQAVDTAADGNFVILEQLARARVFRKLGDHAEVLKSCDEVIRPRRFAWAW
                     GSAIGPCLRWSAEAAAALGNRDVAIGFAKRLLALRMKAPDGDPLVKAAREIAVR"
BASE COUNT          902 a         1688 c         1665 g          924 t
ORIGIN      
        1 gcatgtcctt tcttggttcg aggctcaaca tgcgctccgg cggctcgttt cggtagtggc
       61 ggaaacgaca cttatggtac cgtttccgcc atgcgggtct tcgtggccat cgctgatgac
      121 gtgttcgaca ccgggttcgc ctcgctgctg gacacgttcg agacggcgaa cgagctcgcg
      181 ggtgaatcgc ggttctcggt cacggtcgtg tcgccgcggt cgtcggtgcg tacgcatcat
      241 gggctcgcga ttcgcccgcc gcgccgtccc gcgcgcggac cggatctcgt gatcgtaccg
      301 gcgctcgcgt gcaagcatcc tgcgacgatc gtccgtgcgc tcgaccgccc cgacgtgcag
      361 gcgttgatcg atctcgtcgt gcgctatgcc cgtcggtgtc gcgttgctgc ggcgtgcacc
      421 ggcacgttcg tactcggccg cgccggggtc ctcgacggtc gacgcgcgac cacgacgtgg
      481 tggctcggcc cgacgttccg cgacttgttt ccagccgtcg agctcgaaga agccgagatg
      541 ctgatcgcgg acaagggggt gctgacggct ggcgctgcac tcgcgcacat cgatctcgcg
      601 ctcgcggtga tccgtgagcg cagccccgag ctcgcgagct tggtcgcacg ctacttgttg
      661 atcgaccggc gaccgtcgca ggcaccgttt gtcgcgccgt cgcaagtggc gtgcgacgat
      721 gagctcgtca aacgattcga acgctgggtc cgacagcacc tgggcgagcc gttcgagctt
      781 gcacgcgccg cgcgggcggt tggagcgagc gaacgcacgc tacagcgacg gatccggacg
      841 gtgcttggcc ggccaccgat cgcgttcgtg caggatctga ggctcgagcg cgctgcacac
      901 ttgctgcggg tgacggatga cagtgtcgat gccattgcgg gcgccgtcgg atacgaagat
      961 ggctcgacgc tacgcacgct gctccgccgc aaaatgcgcg tcggcgtgcg cgagctgcgc
     1021 gcgcgttagg gcgattgcgc agcagaccgc gcagggctct cgtcacgccg gcggcaggcc
     1081 gcactgctcc tttgtgtcct tgggatgctt cctgaggcag agcgaatagt cggtgttggt
     1141 gtcctcgcgt tgcccttcga agccgatgcc cttgtagccg atcttcaccg agcgcttggt
     1201 gctgctgggc ggaaggtcct ggtctcgcgc gccaccgccg tcacccgatg accccggcag
     1261 gtcgaatgaa aaagatccgc cacacacgtg cgtgagctcg ctggagtcga tcgtcgaaaa
     1321 tgcatccttg ttcatgttca tcacctttcg tttcagttgc ccggcgcttc gtagctactg
     1381 cagcggctgt gcccgtcggt cgaggagcct gttcgtgcaa cttgcgacga cgaaacacca
     1441 aatctcggcg ccgaaaggag acagatgttg tcgtcgccgg caggcgtaac ctgtgtagcg
     1501 ggaaacgcta cgccgcctcg aggactcgac gaggagcgca tcacggcgac cagacggcaa
     1561 cccgacttaa caccgtagcg ttcgacgcta cacgcgaacg atcttcgcac ctcgcgaacg
     1621 cggtcctgcg cgcgcgttga cgttcctgcg cgcggcgcac agcttgcagc gcggcgggcc
     1681 aaacaacctg cgaggtgtag tcatgacagc catgtcgccg atcgccttat cgctcgtcct
     1741 cacgagctgc gcgcttaccg tcgacgatag agagcttgag gaatcgcccg atgccagcgc
     1801 gaccgtcagc gagcccgagg ccgaaccgtg ggcgatcaaa acgctctctc cgatcagcgg
     1861 cgccaaccag ctccagatca acaacgcatt ggtcggtggt ggcgtcgtac gcctgctcgc
     1921 gggcacgtac gtgatctccg atacgattcg catgacgagc aacagcacgc tccgcggcga
     1981 gcccggcgcg atcatcaagc tcgtcggcga tgcggactgg ccgaagtaca agcccctgat
     2041 tcagggtcaa tccgtttcga acgtgcgaat caccggcctc acgatcgacg gcaacgggtc
     2101 caacaatacg gactccaacg gcgtcagcac ggtgtgcggc aagtacttct atacgctgat
     2161 gcacttcaca agcagcaaga atgtcgaagt cgatcactcg tcgctcggta acaactggaa
     2221 tgatgtcctc aagctcaatt cctcgtcgaa cattcgcttc caccacaaca aggtgcgtcg
     2281 cgaaggacac gacgtcgtgt acgcgatcaa atcagccagc gtgcacgtct atgacaacga
     2341 catcaagatc gcgtgcaaca gcggcgtacg cgccgacggc acgaagccga tgtccatcta
     2401 ccgcaacacg atcacgcgag acggcggcgg ttacgctggg atcgagatcc agggcacgtc
     2461 agaagtgtgg gcctgtaaca acaacatcta cggtcttgtc ggtccgacga tatggaacat
     2521 ctcgggcgcg ccggtccaca cgagcggctg cccacagtga ccaagtcgcc gtgatcagcg
     2581 aagacaggca acgcctgtag cgtcgaacat cacggacggc tcatcgtaca gcgatctcgc
     2641 gcgcggcctt gaccagcggg tcaccgtccg gcgccttcat acgcaacgcg aggagccgct
     2701 tcgcgaaacc gatcgcaacg tcgcggttgc cgagcgcggc tgcagcctcg gcggaccagc
     2761 gcaggcatgg tccgatcgca cttccccacg cccaggcgaa acgacgcggc cggatcactt
     2821 cgtcgcagct cttcagcacc tcggcatggt cgccgagctt tctgaagacc cgcgcgcggg
     2881 cgagctgctc gaggatgacg aagttgccgt cggcagctgt gtcgactgct tgcttccagt
     2941 agtcggccgc gccgttcaag ttcttcgtcg cttccgcgtg aagtgcattg gcgacagcga
     3001 cgacctctgg gaactcgctt ttgcgcgcgg catcgacaag cgtgcgctcg tcgagcgtgc
     3061 ccgccaccag tgtgtgcgcg acaacctggt tgatgttctc agtcgtcagc ttcggcagta
     3121 tcgggcgcag ccgatcccgt cgagcatcgg gcatctcgat agcggcgagc tcgagccagc
     3181 cccgctcgcg ctcgtcgctc ccggtcatca gctcggcggc ggccgctgct ttgtgactcc
     3241 acgcgtcggc tgcgttcacg tcgccaaagg cgttcgccaa cgcgagcaat tgcgacgcgc
     3301 gctgcggcac gtccgcggat gaccaatctg cggcgacgtc ggccgcaagc tcgaactgac
     3361 catcgagcag atagatctcg acgagctcgc gacgcacctc gaacgtcgca cccttgctgt
     3421 cgtgacgacg caagagcgcg atcgccgccg catactcgcg ccgagctaca tgcgcgcgtg
     3481 cctgccagag caggctctct tcgagatcgg gttcgagccg cgcgatcgcc cagcgcatgc
     3541 cgtcgtcgtc cgcgtggcca acgtagtagc ttagcgcgtg cttgaggccg agcttgaacc
     3601 gcggatgacg gagcgcgagc cgccggtata cgaccatcgc ctccgcggga aagccaccgt
     3661 gatagagcgc ctcgaacagg ccgtagttga tttcgcgatg atcggggaag tgcgtcacta
     3721 gctcacggaa gtaattgatc gcgggcgtca ggtgagattg gtcgtagacg agcttgagcc
     3781 cctccatgaa gccgcgttgc gcggattcga ggttgccagc cagcgcccgc gttactgccg
     3841 cgtcgatggg tcgcgagagc caccagttga gcaatgcgcg gtaatactgc gcctcggcga
     3901 ggtcgggcgc ctcgcgctcg accgtgtcca gataaccgtc tgcggctgtg aagtcttgac
     3961 gctcgatcgc ggagatcgcg agctggagat tgcgatcgat ctcgaatttg cgcgcgtcgc
     4021 caagcgcctg aggcgcgcgg cctaccgcga cgcgcgcgac gtcagccgcg atcgagcgcg
     4081 tggccgccgg cacgctgtcg acgctcgtca ttcgttcgaa gcgacccagc tcgccgccgt
     4141 tgatcgtcgt gaccttcacg acgacgcgaa tacctgcctg ctcggtgaaa atctcaccat
     4201 ggatcacggt ctcggcaccg agcgatcgcg cggcgcgttc ccatgcttcc gcaggtgccg
     4261 acccgctggg cagatgatcg agcagctcgt agtaaccgag cacgtggagc tcgggaatcg
     4321 accgcagctg cgtggcaagg atatgcggca acccagaggc agcgaagtcg agccgcgctt
     4381 cacccgtgtg atcgatgaac ggcagcacgg cgatgctcgg ccgctcgtgc gttgcatccg
     4441 ccggtgcagc tgccgttcgg gcggccaccg gcatgtcgtg tccgcgcggt cgcgccaaca
     4501 cgactagcgc accggcggcg atgacagcgg cacctccggc cagccaccaa cgtcgtctcg
     4561 ctggacgttt ggcaacgtca tccgcgtgtt tcgctggacg tttggcagcg tcatccgtgg
     4621 cgagatgccg cgtcgagccg agcgcgtcgg cgaattcgcg tgcgctagcg aatcgatcgt
     4681 cgggctgctc ggcaaccgcg cgtgtgatcg cgctcgcgag cgcggcatca ccagtggccg
     4741 caaggacagc cgcagtaacc tggtcgggtg ctcgactcga cgcgtggagt tgctcgccgg
     4801 tcgccgcctc gaacaacgtc gcgccgaccg cgtacaggtc cgcgcgtacg tcggcgacac
     4861 ggccgcgcag ctgctcggga gccatgtatg ccggcgtgcc aatacgcacg cccgtcgagg
     4921 tcaggtcgcg atcgccgaac gaggcgatgc cgaagtcgac gagcttgatc gtgttagctt
     4981 cgtcgatcag gatgttcgcc ggtttgacgt cgcgatgcac gatcttcgcg gcgtgcgccg
     5041 cggcgagcgc atcgagcaga gactgcccga cgcgaacaat ctcttggatc gatagcacgc
     5101 gctcgcgctt gagctttgcg cgtagcgatt cgccgcggac cagctccatt acgatgaagt
     5161 cgcgctcggt atcgacatc
//
LOCUS       DASUFX010000035         8899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_6070,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000035 DASUFX010000000
VERSION     DASUFX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6070"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1606
                     /locus_tag="VFV99_01355"
     CDS             245..1606
                     /locus_tag="VFV99_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5057968.1"
                     /translation="MLVTRVTNSVAARRHVGAAQLRVLDGPDRGVEIDLPQAGVVIGT
                     DKACDVVLTDAFVSRRHCSVAPTPAGFSITDLGSSNGTVIDGVSVTKVVAPPGVALRI
                     GKTLVQLMPADEVVDIPPSKNDHFGALYGDSVPMRQVFAILERASKSAAPVLFLGESG
                     TGKELMARGVHDASPRAKGPFVVFDCGASTETLIESDLFGHTKGAFTGAAADRVGAFA
                     AAHGGTLFLDEIGDLPVGLQPKLLRMLEAGEVVPLGGRKTEKYDVRVVAATHRDVFGE
                     VARGGFRGDLYYRLAVVEVHVPPLRQRTGDLKRLIEMFLAKAGSPQLASQVSGAALER
                     LERYHWPGNVRELRNVITRAVALAGPDDDFQSLPFVLRPTVAAPEGTSGVSFRADRPF
                     HEAKDEFVAKFEREYLSDLVTKAGGNLSQAARVAGLERKFLYKLLERAGLRSSGQRDE
                     GDQ"
     gene            1603..2955
                     /gene="argG"
                     /locus_tag="VFV99_01360"
     CDS             1603..2955
                     /gene="argG"
                     /locus_tag="VFV99_01360"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5057969.1"
                     /translation="MSRIYRQLPAKGTRLGIAYSGGLDTRCAVAWLAEQGMDVYAYTA
                     DLAQPDEANPADIPPTALAHGAKKARLLDVRDGLVREGLTAIQCGAFHLAVGGKKYFN
                     TTPLGRAVTTTAIVRAMVDDDVHVFGDGSTHKGNDIQRFYRYGILVDPSLKIYKPWLD
                     NAFVKAFGGRTEMAQYLAKIGKPYKTGVEKAYSTDANLLGATHEAKDLERLDAGMRIV
                     EPIMGVAHWKSDVAIKPEEITIGFEQGYPRTINGTRYDAALELFIEANRIGGRHGLGM
                     SDQIENRVIDAKSRGIYEAPGMALLHIVYERLLSAIHNENTLDLYATLGRRLGRLLYE
                     GKWFDPEACMLKDGLTRWVAPSVTGEVTLELRRGDDYTIVATRAEYMSYGPDKLSMEK
                     VAEPAFTPEDRIGALELQNLSVGDNRALLLHHLSSLRQLAPGGGDPLGRLLGESDGDE
                     "
     gene            2971..3942
                     /locus_tag="VFV99_01365"
     CDS             2971..3942
                     /locus_tag="VFV99_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057970.1"
                     /translation="MLRTVTVAVAVAVALAGCGFSHGTLLDDAAPGGDAIDAPIDTID
                     STTQNATCFDKWFAGTIRFATPAPISAINSPVYDRDPFLPADELAVFLSSQRGGGEDD
                     IYVATRATVGDSFSTPMPAYEFNSNDIEGRFSIAANNKIAVVASNRPGGAGGMDVWES
                     IRLNTTDQWPAMNRTNVMMVDTTGNDHDPTLSADGQHLYVAPDTSGTQHIVVATRGTN
                     GKFGTPAPIIQLATTGGEADPWPTADERIIVYSSTAPTTGAGGGDIWYATRASATDTF
                     GTPHIVPDINTTGAEGDPQLSSDGCRIYFSHLVSGTNWDIYIASALP"
     gene            complement(4225..5988)
                     /gene="coaE"
                     /locus_tag="VFV99_01370"
     CDS             complement(4225..5988)
                     /gene="coaE"
                     /locus_tag="VFV99_01370"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5057971.1"
                     /translation="MTAHVIGLTGGIASGKSAVAKLLLAKGAAVIDADKLARDVVAPG
                     QPALAELVARFGAAILATDGTLDRKRLGAIAFSDEQARRDLNRITHPRIAAASAAAIA
                     AWSDAGANVVFYEAALLIENRVHTGLSGVVVVSAPPAVQQQRLMARDSLSAEDALARI
                     AAQLPLAEKVKVATWVIENHGDLDDLTAEVDRVVAEIEAKYGPIKAPGATTSGNGHAV
                     QARPREVALVTGFPAFTARRMIAKLLEKEPDTKLYVLARDKFAPEADHLLETLKAGDR
                     AEVLVGDVCDMDLGLSSVEYRALSKELTWIHHLAGIYFMGVDEVTMRRVNVGGTRTVL
                     DLARDAGRLERLVHWSTTTVSGGRGGTVYEEDLEAGQKFHNGYERTKFEAERLVRNAM
                     RQLPVTVLRPSIIVGDSTTGEIDRLDGPYYLMVLIATNASGLRLPILGRGDSPLHLVP
                     IDYVIEAAWQVAHSRGAAGKTFHLVDPQPLSARDVFEGVAAHAKTEKPRGSIPRPLAR
                     AVLKTPGLARLGRGPLTFLDMIDHMVHYDQTNTAHALAGTTIRCPSLVDYLPVLVQHV
                     LDVARAPATPEQLDEVADPLD"
     gene            6025..6468
                     /locus_tag="VFV99_01375"
     CDS             6025..6468
                     /locus_tag="VFV99_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5057972.1"
                     /translation="MLEKLLLAVTAIGALVGVAWASPRDWVGSAKCGSCHPKQLAAWQ
                     ATPHATTRQRFPGKPEARCLGCHGTGEAPAGPSAVEVGCEACHGAGAAYAADDLMRNR
                     TAALALGLADLSTPKLRATLCMTCHARMTKSRAFDATAPVHPVKP"
     gene            6465..7040
                     /gene="yihA"
                     /locus_tag="VFV99_01380"
     CDS             6465..7040
                     /gene="yihA"
                     /locus_tag="VFV99_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5057973.1"
                     /translation="MKVNARFVTSAAESKQFPPEKLPEIAVVGRSNVGKSSLINSLTG
                     KDGLARTSRTPGRTRLINWFEIDGKFQLVDLPGYGYAEVSRDMRESWRPLIEAYLAER
                     KTLAGVLLLIDVRRGVQEEELDFVPWLEARETSIVVALTKADKLAKNKRMLEVAKAKK
                     ALGLKRDPIAVSAQTGDGIDPLWRAVLGLLK"
     gene            7037..7489
                     /gene="rimI"
                     /locus_tag="VFV99_01385"
     CDS             7037..7489
                     /gene="rimI"
                     /locus_tag="VFV99_01385"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5057974.1"
                     /translation="MSIVVAPATEADLDAIDEIEQHSFKSPWPRDTFKAELLREWARL
                     DVGRIDGKLVVFCNYWLVTTEVHILAIATHPDYRGRGLARQLLDHILGVARQTGCSLA
                     TLEVRRSNVPAIALYERAGFKTVHVRARYYQDDNEDALVMLKAITEPG"
     gene            complement(7490..8311)
                     /locus_tag="VFV99_01390"
     CDS             complement(7490..8311)
                     /locus_tag="VFV99_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5057975.1"
                     /translation="MRAALALAILCALAPSARADVNPRRKVVVIEYRAGSSALPKIAV
                     DLVAEMAKQTSIQVLGPDQTKALYGEQLDQAIVKCAGEADCVAKIGAKVGAAEVVLVG
                     ISELGDVILTMQRIDVRTHAVNARIADSLAANSEPNSDQLGYYLTKLLPPSDFLRFGI
                     IAITSNEAGAAVTVGGEKRGLTPIEPLKLRAPAKYPIRIEKPGFVPFTTTVQLPPDSE
                     LKVEAELSLRGGGGTHWYQKWYVYAGGVVVLGLVGGGVYFATREPPNSVPVGGSL"
     gene            complement(8308..>8899)
                     /locus_tag="VFV99_01395"
     CDS             complement(8308..>8899)
                     /locus_tag="VFV99_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057976.1"
                     /translation="LGDRVYAAGTRPSVPMCATGMCSSTSSTACPETSTNRVSYIADG
                     ARLLVQSLPIAGGNAITLELPERRETMVDTNDGARSHAQVLHPFGTVPLDLVALPGGQ
                     YVSVVTKNSYYIESATDATFGVILPCLKTSTSDWLLVDMASSSIAQRVRTHCMITNVR
                     PGALFPNWACDDPPEAEQSTQGDYQPVSVGALFGVR"
BASE COUNT         1465 a         2989 c         2997 g         1448 t
ORIGIN      
        1 cacgtggacg tctacgtgca cgacctctga gtgccgtccg aactgcgtcg ccggctgtca
       61 cgccgacact gcgcgctcga tcgctggcac cagtgtttcc gagccagaga attcgattgt
      121 cgcgagtccg tttcgtgcac cgacgtttcg gctggcgcaa cgcaaatgtg ccgcgccccc
      181 gatcgacgac gtgcacgtgc acgtgcacgt ggcacgtgta cgaacacgga aagatgtggt
      241 cgccgtgcta gtcacccgcg tcacgaactc cgtcgctgcg cgcagacatg tcggcgcggc
      301 ccagctgcgc gtcctcgacg gtccggatcg tggcgtcgag atcgatctgc cgcaggccgg
      361 cgtcgtgatc ggcaccgaca aggcgtgcga cgtcgtgctc accgacgcgt tcgtgtcgcg
      421 ccggcactgc tcggtcgcac cgacgcccgc gggcttctcg atcaccgatc tcggctcgag
      481 caacggcacc gtgatcgacg gcgtctcggt cacgaaggtg gtcgcaccgc ccggcgtcgc
      541 tctgcgtatc ggcaagacgc tcgtgcagct gatgccggcg gacgaggtcg tcgatattcc
      601 gccgtcgaag aatgatcact tcggcgcgct gtacggcgac tcggtgccga tgcggcaggt
      661 gttcgcgatc ctcgagcgcg cgagcaagtc ggcagcgccg gtcctgttcc tcggcgagag
      721 cggcaccggc aaggagttga tggcgcgcgg cgttcacgac gcatctccgc gggccaaggg
      781 cccgttcgtc gtgttcgatt gcggcgcttc gaccgagacg ctgatcgaga gtgacctgtt
      841 cggtcacacg aagggcgcgt tcacgggtgc tgcggcggat cgcgtcggtg cattcgcggc
      901 cgcgcacggc ggcacgttat tcctcgacga gatcggagac ctgccggtcg gcttgcagcc
      961 gaagcttctt cgcatgctcg aagccggcga ggtcgtgccg ctcggcggtc gcaagaccga
     1021 gaagtacgat gttcgcgtcg ttgccgcgac tcaccgcgac gtgttcggcg aagtcgcacg
     1081 cggcggcttc cgcggcgatc tgtactaccg gctcgccgtc gtcgaggtcc acgtaccacc
     1141 gctgcgccag cgcacgggcg acctcaagag actcatcgag atgttcctcg ccaaggcagg
     1201 ctcgccgcag ctcgcgtcgc aagtcagcgg cgccgcgctc gagcggctgg agcgctatca
     1261 ttggccgggc aacgtgcgcg agctccgcaa cgtgatcacc cgcgcggtcg cgctcgccgg
     1321 tcccgacgat gacttccaat cgctgccgtt cgtgctccgg cctaccgtcg ccgcgcctga
     1381 aggaacgtcg ggcgtgtcgt ttcgcgccga tcgaccgttc cacgaggcca aagacgaatt
     1441 cgtcgcgaag tttgaacgcg agtatctgag cgatctggtg accaaggcgg gcggcaacct
     1501 gtcgcaagcc gcacgcgtcg ctggcctcga gcgcaaattt ctgtacaagt tgctcgagcg
     1561 cgctggcctt cgttcctcag ggcagcgcga cgaaggtgac caatgagtcg aatctatcgt
     1621 cagcttccag cgaagggcac gcgcctcggc attgcgtact ccggcggcct cgacacgcgc
     1681 tgcgcagtcg cctggctcgc cgagcagggc atggacgtct acgcgtacac cgctgatctc
     1741 gcccagcccg acgaagcgaa tccggccgat attccgccga cggcgctggc gcacggcgcg
     1801 aagaaggcgc ggctgctcga tgttcgcgac ggcctcgtgc gcgaaggtct caccgcgatc
     1861 cagtgcggcg catttcacct tgccgtcggc ggcaagaagt acttcaacac cacgccgctc
     1921 ggccgcgcgg tgacgacgac cgcgatcgtg cgcgcgatgg tcgacgacga tgtccacgtg
     1981 ttcggcgatg gctcgacgca caagggcaac gacatccagc gcttctatcg ttacggcatc
     2041 ctcgtcgatc cgtcgctcaa gatttacaag ccgtggctcg acaacgcgtt cgtcaaagcg
     2101 ttcggcggcc gcaccgagat ggcgcagtac ctcgcgaaga tcggcaagcc gtacaagacg
     2161 ggcgtcgaga aggcgtactc gacggacgcg aacctgctcg gcgctacaca cgaggcgaaa
     2221 gacctcgagc gcctcgacgc cggcatgcgg atcgtcgagc cgatcatggg cgtcgcgcac
     2281 tggaagtccg acgtcgcgat caaacccgaa gagatcacga tcggcttcga acaaggctat
     2341 ccgcgcacga tcaacggcac gcgctacgac gcagcgctcg agctgttcat cgaggcgaac
     2401 cgcatcggcg gccgccacgg cctgggcatg agcgatcaga tcgagaaccg cgtgatcgac
     2461 gccaagagcc gcggcatcta cgaagcgccg ggcatggcgc tgctccacat cgtctacgag
     2521 cgcctgctca gcgcgatcca caacgagaac acgctcgacc tctacgcgac gctcggtcgc
     2581 cggctcggcc gcttgctcta cgagggcaag tggttcgacc cggaggcatg catgctgaag
     2641 gacggcctca cgcgctgggt ggcaccgagc gtgaccggcg aggtgacgct cgagctgcgc
     2701 cgcggcgacg actacacgat cgtcgcgacc cgcgccgagt acatgtcgta cggccccgac
     2761 aagctgtcga tggagaaggt cgccgagccc gccttcacgc ccgaggatcg aattggtgcg
     2821 ctagagctgc agaacctgtc ggtaggcgac aaccgcgcac ttctgctcca tcacctgtcg
     2881 agcctgcgcc aacttgcgcc gggtggtggc gatccgctag gtcgtttgct tggcgagtct
     2941 gacggcgacg aatagcgtta tcctgatcac gtgctccgga cggtgacggt ggcggttgcg
     3001 gttgcggtcg cgctggcggg ttgcggcttc tcgcatggca cgttgctcga cgatgcagcg
     3061 ccgggcggcg atgccatcga tgcgccgatc gacacgatcg acagcacgac gcagaacgcg
     3121 acctgcttcg acaagtggtt cgccggcacg atccgcttcg ccacaccggc cccgatcagc
     3181 gcgatcaatt cgcccgtcta cgatcgcgat ccgttcctgc cggcggacga gctcgccgtc
     3241 tttctcagct cccagcgcgg cggaggcgag gacgacatct acgtcgcgac tcgcgccacg
     3301 gtcggcgact cgttctcgac gccgatgccg gcctacgagt tcaacagcaa cgacatcgag
     3361 ggcagattct cgatcgcggc gaataacaag atcgccgtcg ttgcgagcaa tcgacccggc
     3421 ggggcgggcg gcatggacgt gtgggagtcg atccggctca acaccaccga ccagtggccg
     3481 gcgatgaatc gcacgaacgt gatgatggtc gacacgaccg gcaacgatca cgatccgacg
     3541 ctgtccgcgg acgggcagca tctatacgtc gcgcccgaca cgagcggtac gcagcacatc
     3601 gtggtcgcga cgcgtggcac gaatggcaag ttcggcacgc cggccccgat tattcagctc
     3661 gcgactaccg gcggcgaggc cgacccgtgg ccgaccgctg acgagcgcat catcgtctac
     3721 tcgtccacgg ccccgacgac cggcgcaggc ggcggcgata tttggtacgc gactcgcgcg
     3781 tcggcgaccg acacgttcgg cacgccgcac atcgtgccgg atatcaacac gacgggagcg
     3841 gagggcgacc cacagctgtc gagtgacggc tgccggatct acttctcgca cctggtctcg
     3901 ggcacgaact gggacatcta catcgccagc gcgctgccgt aggactgcgc acgcgtagcg
     3961 tcgaacgagc ccggcgtcag cggctacgcc gctcccagat ggaacgcgaa gatgccaagc
     4021 cggccaagaa cgccaagtga aaggcttcgg gatcgcgttc tgctcgcacg accgcgcctg
     4081 cagaccgcgt cgaagaaccg ccttctggtg gcgttcttgg cgggctgcgc ttggcggtct
     4141 tggcgttaga tctgaaacac gtcgtgcggc gccgcaggcg ccattcggct ccgacggcga
     4201 cggtcgtgcg tctcgcactc gagcctagtc cagcgggtcg gcgacttcgt cgagctgttc
     4261 gggcgtcgcc ggtgcgcgcg cgacatcgag tacatgctgc acgagcaccg gcaggtaatc
     4321 gacgagcgag ggacagcgaa tcgtcgtgcc cgcgagcgcg tgggccgtgt tcgtctgatc
     4381 gtagtgcacc atgtgatcga tcatgtcgag gaacgtgagc ggaccgcggc cgaggcgcgc
     4441 gagacctggg gtcttcagca cggcgcgcgc gagcggacgc gggatgctgc cgcgtggctt
     4501 ctcggtcttc gcgtgagcgg cgacaccctc gaacacgtcg cgcgccgaca gcggctgcgg
     4561 atcgacgagg tggaacgtct tgccggccgc gccgcggcta tgcgcgacct gccacgccgc
     4621 ctcgatcacg taatcgatcg gcacgaggtg aagaggggaa tcgccgcggc cgaggatcgg
     4681 aagccgcagt cccgacgcgt tcgtcgcgat gagcaccatc aagtagtacg ggccgtcgag
     4741 tcgatcgatc tcgccggtcg tgctgtcgcc gacgatgatc gatgggcgca gcacggtgac
     4801 cggcagctgg cgcatcgcgt tgcggacgag tcgctcggcc tcgaatttcg tgcgctcgta
     4861 gccgttgtgg aacttctgac cggcttcgag atcctcctcg tagacggtgc cgccgcggcc
     4921 gccggagacc gtcgtcgtcg accagtgcac gagccgctcg agtcggccgg cgtcgcgcgc
     4981 gagatcgagc acggtgcgcg tgccgccgac gttgactcgc cgcatcgtga cctcgtcgac
     5041 gcccatgaag tagatgccgg cgaggtgatg gatccaggtc agctctttcg agagcgcgcg
     5101 gtactcgacg ctcgacagcc cgaggtccat gtcgcagaca tcgccgacga gcacctccgc
     5161 gcgatcgccc gccttgagcg tctcgagcaa gtggtcggct tcgggggcga acttgtcgcg
     5221 cgcgagcacg tagagcttgg tgtcgggctc cttctcgagg agcttcgcga tcattcgccg
     5281 tgcggtgaac gcggggaatc cggtgacgag cgcgacctcg cgcggccgcg cctgcacggc
     5341 atgcccgttg ccagacgtcg tggcgcccgg cgccttgatc gggccgtact tggcttcgat
     5401 ctcggcgacg acgcgatcga cttcggccgt gaggtcgtcg agatcgccgt ggttctcgat
     5461 cacccacgtc gcgaccttca ccttctcggc gagcggtagc tgcgcggcga tccgtgcgag
     5521 cgcgtcctcg gccgatagcg agtcgcgcgc catgaggcgc tgctgctgca ccgccggagg
     5581 cgccgacacg acgaccacac cggagaggcc ggtgtggacg cggttttcga tcaacagggc
     5641 cgcctcgtaa aacacgacgt tcgcgcccgc gtcggaccac gctgcgatcg ccgccgcgct
     5701 cgccgccgcg atgcgcggat gggtgatgcg gttgagatcg cggcgcgcct gctcgtcgga
     5761 gaacgcgatg gcgccgaggc gcttgcggtc gagcgtgcca tcggtggcaa gaatcgcagc
     5821 gccaaaccgc gcgaccagct cggcgagggc aggttgccca ggcgcgacga cgtcgcgggc
     5881 cagcttgtcg gcgtcgatga cggccgcccc cttcgcgagc aacagtttgg cgactgcgct
     5941 ctttcccgac gcgattccgc cggtcagccc gatcacatgc gccgtcacgg cgttgagtta
     6001 ccacgggtct ggtaggtttg gctcgtgctc gagaagttgc tgctggcggt gaccgcaatc
     6061 ggcgcgctcg tcggcgtcgc gtgggcgtcg ccgcgcgatt gggtcggcag cgcgaaatgc
     6121 ggttcgtgcc accccaagca gctggcggct tggcaggcga ccccacacgc aactacgcgc
     6181 cagcgcttcc ctggtaagcc ggaagcccgc tgcctcggct gccacggcac cggtgaggcg
     6241 cccgcaggcc cgagcgccgt cgaagtcggc tgcgaggctt gccacggcgc cggcgcggcc
     6301 tacgctgccg acgatctcat gcgcaaccga accgctgcct tggccctcgg cctcgccgat
     6361 ctgtcgacgc cgaagctgcg cgccacgctg tgcatgacct gtcacgcgcg gatgacgaag
     6421 tcgcgcgcat tcgacgcgac cgcgccggtc cacccggtga agccatgaaa gtcaacgcgc
     6481 gcttcgtgac gtcggctgcc gagtcgaagc agtttcctcc cgagaagctg cccgagatcg
     6541 ccgtggtcgg ccgcagcaac gtcggcaagt cgagccttat caactcgctg accggcaagg
     6601 acggtctcgc gcgcacgtcg cggacgccgg gacgcacgcg gctcatcaac tggttcgaga
     6661 tcgacggcaa gttccagctc gtcgacctgc cgggctatgg ctacgccgag gtcagccgcg
     6721 acatgcgcga aagctggcgc ccgctgatcg aagcgtacct cgccgagcgc aagacgctcg
     6781 ccggtgtgct gctactcatc gacgtgcggc gcggcgtgca ggaggaggag ctcgacttcg
     6841 tgccgtggct cgaagcgcgc gagacgtcga tcgtcgtcgc attgacgaag gccgacaagc
     6901 tcgcgaagaa caagcgcatg ctcgaggtgg cgaaggcgaa gaaggcactc ggcctcaagc
     6961 gcgatccgat cgcggtgtcg gcgcagacgg gcgacggcat cgatccgctg tggcgtgcag
     7021 tactcggact gctcaagtga gtatcgttgt cgcgccggct accgaagccg atctcgacgc
     7081 gatcgacgag atcgagcagc actcgttcaa gtcgccatgg ccgcgcgaca cgttcaaggc
     7141 ggagctgctc cgcgagtggg cgcgtctcga cgtcggccgc atcgacggca agctcgtcgt
     7201 gttctgtaac tactggctcg tcacgaccga ggtgcacatc ctcgcgatcg cgacgcatcc
     7261 cgactaccgc ggccgcgggc tcgctcgtca gctgctcgac cacatcctcg gcgtcgctcg
     7321 ccagaccggt tgctcgctcg ccacgctcga ggtccgccgc tcgaacgtac cggcaatcgc
     7381 gctctacgaa cgcgcgggct tcaagaccgt gcacgtccgc gcgcgctact accaggatga
     7441 caacgaggac gcgctcgtga tgctcaaggc gatcacggag ccgggctagc tagagcgagc
     7501 cgccgaccgg cacgctgttc ggtggctcgc gcgtcgcgaa gtacacgccg ccaccgacga
     7561 gaccgagcac gacgacgccg cccgcgtaca cgtaccactt ctggtaccag tgcgtgccgc
     7621 cgccaccgcg aagcgacagc tcggcttcga ccttgagctc gctgtcggga gggagctgca
     7681 cggtcgtcgt gaacggcacg aagcccggct tctcgatccg gatcggatat ttcgccggtg
     7741 cgcgcagctt gagcggctcg atcggcgtca ggccgcgctt ctcgccgccg acggtgaccg
     7801 ccgcgcccgc ttcgttcgac gtgatcgcga tgatgccgaa ccgcaagaag tccgacggcg
     7861 gcaacagctt ggtgaggtag tagccgagct ggtcgctgtt cggctcgctg ttcgctgcga
     7921 gcgagtccgc gatgcgggcg ttcaccgcgt gcgtgcggac gtcgatgcgc tgcatcgtga
     7981 ggatcacgtc gcccagctcg ctgatgccga cgagcacgac ctccgcagcg ccgaccttcg
     8041 cgccaatctt cgcgacgcaa tccgcttcgc cggcgcactt cacgatcgcc tgatcgagct
     8101 gctcgccgta cagcgccttg gtctggtcgg ggccgagcac ctggatcgag gtctgcttcg
     8161 ccatctcggc gacgaggtcg accgcgatct tcggtagcgc cgacgagccg gcgcgatatt
     8221 cgatgacgac gaccttgcgc cgcggattga catcggcgcg cgccgatgga gcgagtgcgc
     8281 acaggatcgc cagggcgagc gccgctctca tcgcactcca aacagcgcgc cgacggagac
     8341 cggctggtaa tcgccttgcg tcgactgttc tgcctcgggc gggtcgtcgc aggcccagtt
     8401 cgggaacagc gcgcctggcc gcacgttcgt gatcatgcag tgcgtgcgta cgcgctgtgc
     8461 gatcgagctc gacgccatgt cgacgagcag ccagtcggag gtgctcgtct tcaggcacgg
     8521 caggatcacg ccgaacgtcg cgtcggtcgc gctctcgatg taatagctgt tcttcgtcac
     8581 gaccgagacg tattgcccgc cgggcagggc gacgaggtcg aggggcacgg tgccgaatgg
     8641 atgtagcacc tgcgcgtgcg agcgcgcgcc gtcgttggtg tcgaccatcg tctcgcgacg
     8701 ctccggcagc tcgagcgtga tcgcgttgcc gcccgcgatc ggcagcgact gcacgagcag
     8761 ccgcgcgccg tcggcgatat acgagacgcg gttcgtgctc gtctccgggc acgcggtcga
     8821 gctcgtcgag ctgcacatac cggtggcgca catcggcacc gacggccggg tgccagccgc
     8881 gtagacgcga tcgccgagt
//
LOCUS       DASUFX010000036         7617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1951721, whole genome shotgun sequence.
ACCESSION   DASUFX010000036 DASUFX010000000
VERSION     DASUFX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951721"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3086
                     /locus_tag="VFV99_01400"
     CDS             <1..3086
                     /locus_tag="VFV99_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5057977.1"
                     /translation="PGGEPFYAMPLVSGRSLDKVVAEKKTLNDRLGLLPNVIAVADAL
                     AYAHNSNVIHRDLKPANVLVGEFGETVVIDWGLAKDLGVYTDPKESLQMRLRATAEET
                     MSGSVVGTPTYMPPEQARGDAVDQRADVYALGALLYKVLSGVAPYTGESALGVLEAVK
                     DGPPVPVQEREPEAPPDLVAIVAKAMARNPDDRYITASELAQDLKRFETGQLVGAHRY
                     TTGELMRRWLRRHRVAVAISAVALITLIVVGVFSLQRIVAAKKDADAAKVDAEHQRDH
                     AIAGRVNLLEERARTLLDKNAGHALAYLVGAAEVETQRIPGAQLSNRARGFMIADAIA
                     PFEAELAHIDVATGNVVVATSPNGKWVAAAGNGPIRLLDHTGKLTKSLGAFGTTRVVA
                     FDAASTRLVAAGDDGIVRIWSLEHEPIDTRPIAELRGHTGAVNDAVFNNDGKLLATGG
                     DDKSVRIWNLEAHTSKPVVCHGLPVVSVRFSPTAPKVLSASADFSACVIAFRDAGPYP
                     WIIERQIRGHDARVNSAEWSSDGKFIVTASDDGTARVWSEARGKPVYRDTLSHEPGAL
                     VKRALVTRSGSIISAGSDLAIRIWELPSTVPKDGTTPPRVKPPKQLEGHTGQIVAAAL
                     SADEHYLATGGFDGLVKIWDLGSRQQVAMFEPGAVVTSLAFIPGDPSRVVTGSADGKA
                     RIWNASVGERSLDLGAAVRAIAVGPNGKVVAGLGDSLVRVVERGATTDTVDLHRQLGA
                     VNAVAFVANDKLITAGEDPAVFVWDLDPKAPKQLLGTFAETKLDPPPPIRAVARVPSN
                     DGDRVALVRAGRIELWRLANDLSKSGLVGTLAPEHGAIDTIAVSAQGMIVGVGQDGTF
                     AVWDKTGSQLAASGKHDPSYNAVAFAPDGSALVAAGPGFARVFDVTSKGIGDVAITLD
                     PSEDIGVVRTVLFAAEGALVITAGDRGRALIWDRQKGKLLARRDRHDAAINALGMDGE
                     ILWVASEDQTLRAWKVHVDTRTLEELQDFMIDHVAWTLKDDVVVRVEDATGKQGP"
     gene            3083..4816
                     /locus_tag="VFV99_01405"
     CDS             3083..4816
                     /locus_tag="VFV99_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5057978.1"
                     /translation="MSESVEQTASRLARFGDVEGGPGSAYLLVVDNDSSSIFHLPHSG
                     AIVIGRGNDVELKVQHASVSRRHATIRVDEGTVRIADLGSHNGTRVNGELVADARTLA
                     SGDVATVGDVVLVIHFSTPAVVARAAYAEAGWRRRLAEELVRAITFKRALGVVVIQGA
                     SAASLGANLRLIDVIGTSDDGQALLLLPEVDREQAREIAEKAIALVPNARGGLAMCPT
                     DACDADTILLAARSAARRAAAGAIADAAESATKIELGSRHVLVADPAMSRVFALLERL
                     AVAELPVLITGETGVGKENAAYAVHHWSKRSGPFLPVNCAAIGPESLVDAELFGYEKG
                     ADTGATAAKAGLFESAAGGTVFLDEVGELPLAIQAKLLRALEVKRITRLGETRERAID
                     VRLVAATNRSLEEEVKAGRFRQDLYFRLSGATVILPPLRDRQCEIPLLARAFLVDACS
                     RSGRDRMTITPAAMQVLLTYSWPGNVRELRNTMEYVMAAAPDDNVEPLDLPERLGGSA
                     APPTAAAVPHDVVADGKTTFRPINDELKELERRRMAEALAAAGGVKTKAAQLIDMPIR
                     TFTLKLKQYKL"
     gene            4951..5451
                     /locus_tag="VFV99_01410"
     CDS             4951..5451
                     /locus_tag="VFV99_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057979.1"
                     /translation="MRNFLLVILVGASACAARSSNQPTLPVHYACGEATVTRNGIEMR
                     SSATPGEISRLSWHDDKGEHFVTWPLSPTDRTATEFVVPSDPRQDATQRTYDTTFGSS
                     TADWRLTDKQVCTARGGYNDLLARYMRGESIDDLTRDVGLSSREETRAVIRKALVSLQ
                     KKYWSE"
     gene            complement(5519..5977)
                     /locus_tag="VFV99_01415"
     CDS             complement(5519..5977)
                     /locus_tag="VFV99_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5057980.1"
                     /translation="MIWKTILVPHDFSSSANHAVAIARDEAKAHGANLLLLHVIDLPT
                     AIKPDITIVPDENGAPINIKDYAIKQAEAHLADLAARVAKDGVAAQTFLRIGKPEDEI
                     VKFATENKVDLIAMGTHGRTGLEHLLVGSVAERVVRMSPCAVLTIRSAKE"
     gene            complement(5999..6742)
                     /locus_tag="VFV99_01420"
     CDS             complement(5999..6742)
                     /locus_tag="VFV99_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5057981.1"
                     /translation="MGTACDRKAATPPAASATNAIAPRGFAIPKQTKQLVIAIVDDWT
                     TTHARLALWQRTEAGWQRVGDDWPAVIGKSGSGWGIGLYPSAPNREGPTKKEGDGKSP
                     AGVFAFRSSYGYADDAPKGWRMPYEQSTDLECVDDAASEHYTKIIDRKQVASDWQSAE
                     QMRRDDEAYEWVLDVAHNPDAKPGAGSCIFLHVWSGPESTTVGCTAMEKPKLEALLEK
                     LDPASQPLYLLLPRSEYNAVATSWGLPAL"
     gene            complement(6790..>7617)
                     /gene="nadA"
                     /locus_tag="VFV99_01425"
     CDS             complement(6790..>7617)
                     /gene="nadA"
                     /locus_tag="VFV99_01425"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5057982.1"
                     /translation="DFVGDSLQLSQAAAKTKADVIVFCGVHFMAETAKILNPDKIVVV
                     PDLSAGCSLADGCPAPVFKQWLEKYPGHTVVSYINCSADVKALSDIICTSSNAVKIVK
                     SLEGQKVVFAPDRHLGAFVAKQANRPDLVTWQGTCIVHETFSEKRLLELMAQHPDAEV
                     IAHPECTAEILRHANHIASTSGLIEYAKKSAKQTFIVATEAGIMHKMQQAAPGKTLIA
                     APPEDESCSCNMCPFMRKNTLEKLYLCMRDLQPQVDVPEETRIKALRPIQRMLEMSA"
BASE COUNT         1291 a         2395 c         2608 g         1323 t
ORIGIN      
        1 ggccgggtgg tgagccgttc tacgcgatgc cgctcgtgtc gggacgttcg ctcgacaagg
       61 tcgttgcgga gaagaagacg ctgaacgacc ggctcggcct cttgcccaac gtgatcgcgg
      121 tcgcggacgc actcgcgtac gcgcacaact cgaacgtcat ccatcgcgac ctcaagccgg
      181 cgaacgtgct cgtcggtgag tttggcgaga cggtcgtgat cgactggggc cttgcgaagg
      241 atctcggcgt ctacaccgat ccgaaggagt cgctgcagat gcggctgcgc gcgaccgccg
      301 aggaaacgat gtcggggagc gtcgtcggca cgcccacgta catgccgccg gagcaagcgc
      361 gtggcgatgc ggtcgatcag cgcgcggacg tctacgcgct cggcgcactg ctctacaaag
      421 tgctttccgg tgtagcgccg tacactggtg agagcgcgct cggcgtactc gaggcagtga
      481 aggacgggcc tccggtaccg gtgcaggagc gcgagcccga agcgccaccg gatctcgtcg
      541 cgatcgtcgc gaaggcgatg gcgcgcaatc ccgacgatcg ttacatcacg gcgagcgagc
      601 tcgcgcagga cctgaagcga ttcgagaccg gtcagctcgt gggtgcgcat cgctacacga
      661 ccggcgagct gatgcggcgg tggttgcgcc ggcatcgcgt cgcggttgcg atcagtgcgg
      721 tcgcattgat cacgttgatc gtggttggcg tgttctcgtt gcagagaatc gtcgcggcga
      781 agaaggatgc cgacgcagca aaggtcgatg cggagcacca gcgcgaccac gcgattgccg
      841 gtcgagtcaa cctcctcgag gaacgtgccc gcacattgct cgacaagaac gccggtcacg
      901 cgctcgcgta cctcgtcggt gcagcagagg tggagacgca gcgcatacct ggtgcgcagc
      961 tttcaaatcg cgcgcgtggg ttcatgatcg cggacgcaat cgcgccgttc gaagccgagc
     1021 tcgcgcacat cgacgttgca accgggaatg tcgtggtggc gacgagcccg aacggcaagt
     1081 gggtcgccgc cgccggtaac ggtccgatcc ggctgctcga ccacacgggc aagcttacga
     1141 agtcgctcgg cgcgttcggg accacgcggg tggtggcgtt cgatgcggcg agcacgcggc
     1201 tcgtagccgc aggtgacgac ggaattgttc gaatctggtc actggagcac gagccgatcg
     1261 acaccaggcc gatcgcagag ctccgtggtc acaccggcgc ggtcaacgac gcggtgttca
     1321 acaatgacgg caagctgctc gccaccggtg gcgacgacaa gagcgtccgg atctggaacc
     1381 tcgaagctca taccagcaaa cccgtcgtgt gccacgggtt gccggtcgtg tcggtgcgat
     1441 tctcgccgac ggcaccgaaa gtgctaagtg caagcgccga cttcagtgca tgcgtgatcg
     1501 cgttcaggga tgcggggccg tacccatgga tcatcgagcg gcagatccgc ggccacgatg
     1561 cgcgcgtcaa cagcgccgag tggagcagcg atggcaagtt catcgttacc gcgagcgacg
     1621 acggaacggc gcgcgtgtgg agcgaagcga gaggcaagcc ggtctatcgg gacacgctga
     1681 gccacgaacc gggcgctctc gtcaaacgtg cgctcgtcac gcgcagcgga tcgatcatca
     1741 gcgcgggtag cgacctcgcg atacggatct gggaattacc gtcgacggta ccgaaggacg
     1801 gaaccacgcc accgcgggtc aaaccgccaa aacagctgga gggtcacacc ggtcagatcg
     1861 tcgccgctgc gctatcggcc gacgagcact atctggcgac cggcggtttc gacggcctcg
     1921 taaagatctg ggatctaggc tcgcggcagc aggtcgcgat gttcgagccc ggcgcggtcg
     1981 tcacgtcgct cgcgttcatc ccgggcgacc cgagtcgtgt cgtgaccggt agcgcggatg
     2041 gcaaggcgcg catctggaac gcatcggtcg gcgagcgttc actcgatctc ggagccgcgg
     2101 tgcgcgcgat cgccgtggga cctaacggta aggttgtcgc cggtcttggc gacagcctcg
     2161 ttcgcgttgt cgaacgcggc gcgactaccg acacagtcga tctccaccgt caactcggcg
     2221 ccgtcaatgc ggtcgcgttc gtcgcaaacg acaagctgat cacggccggc gaagatccgg
     2281 ctgtgtttgt ctgggatctg gatccgaaag cgccgaagca gttgctcggc acgtttgcag
     2341 aaacgaagct cgatccaccg ccgccgatcc gcgcggtcgc cagggtgccg agcaacgatg
     2401 gcgatcgggt ggcgctagtt cgcgcgggca ggatcgagct gtggcggctc gctaacgatc
     2461 tttcgaagag tggcctcgtg ggcacgctcg cgcccgagca cggtgcgatc gatacgatcg
     2521 cggtgagcgc gcaggggatg atcgtcggcg tcggtcagga cggcacattc gcggtgtggg
     2581 acaagacagg gagccaactg gcggcgtccg gcaaacacga ccccagctac aacgccgtgg
     2641 cgtttgcccc cgacggtagt gctctcgttg cagccggtcc tgggttcgcg cgtgtattcg
     2701 acgtcacgag caaagggatc ggcgacgtcg cgatcacact cgatccatcc gaggatatcg
     2761 gtgtcgtacg cacggtgctt ttcgccgcgg agggcgcgtt ggtgatcacc gctggtgatc
     2821 gcggccgcgc gctcatctgg gaccgtcaga agggcaaact gctcgcgcgg cgtgatcggc
     2881 acgatgcggc gatcaacgcg ctcggaatgg acggcgagat tctttgggtc gcgagcgagg
     2941 atcagacgct gcgtgcgtgg aaggtgcacg tcgacacgcg cacgctcgag gagctgcaag
     3001 atttcatgat cgaccatgtg gcctggacgc tcaaggacga cgtggtcgtc cgcgtcgagg
     3061 acgcgactgg aaagcaagga ccatgagcga gagcgtcgaa caaacagcaa gccggttggc
     3121 gcgcttcggc gacgtcgaag gcgggccggg cagtgcgtat ctgctcgtcg tcgataacga
     3181 cagctcgtcg atcttccacc tgccgcactc gggcgcgatc gtgatcggcc gtggcaacga
     3241 cgtcgagctc aaggtccagc acgcctcggt ctcgcgccgg catgcgacga ttcgcgtcga
     3301 cgagggcacg gtgcgcatcg cggacctcgg cagccacaac ggcacgcgcg tcaacggcga
     3361 gctggttgcc gacgcgcgca cgctcgcatc gggggatgtc gcgacagtcg gcgatgtcgt
     3421 gctcgtcatt cacttctcga cgcccgccgt cgtcgcgcgt gcggcgtatg cagaagctgg
     3481 ctggcggcgc cggctcgccg aggagctggt tcgcgcgatc acgttcaagc gcgcgctcgg
     3541 cgttgtggtc atccagggtg cgtcggcggc atctctcggc gcgaacctcc gtctcatcga
     3601 cgtgatcggc acgagcgacg acggccaggc gctgctgcta ttgcccgagg tcgatcgcga
     3661 gcaagcgcga gagattgccg agaaggcgat cgcgctcgtg ccgaacgcgc gcggtggtct
     3721 cgcgatgtgc ccgaccgatg cgtgcgacgc cgatacgatc ttgctcgcgg cgcgatccgc
     3781 agcgcggcgg gcggccgcag gagccatcgc ggatgcggcg gagagcgcga caaagatcga
     3841 gctcggctcg cgccacgtgc tggtcgctga tccggcgatg agccgcgtgt tcgcgttgct
     3901 cgagcgactc gccgtggcag agctgccggt gctgatcacc ggcgagactg gcgtcggcaa
     3961 agagaatgcg gcctatgcgg tccatcattg gtccaagcgc agcggtccgt tcctgcccgt
     4021 caattgcgcg gcgatcggac cagagtcgct cgtcgacgcc gagctgttcg gctacgagaa
     4081 gggcgcggac accggcgcga cggcggcgaa ggccggtctg ttcgagagcg cggccggcgg
     4141 cacggtgttt ctcgacgagg ttggcgagct gccactcgcg atccaggcga agctgttgcg
     4201 cgcgctcgag gtcaagcgca tcacccggct cggcgagacc cgggagcgcg cgatcgacgt
     4261 ccggcttgtc gcggcgacga accgttcgct cgaagaagag gtgaaggcag gccgctttcg
     4321 ccaggatctc tatttccggc tctccggcgc gaccgtcatc ttgccgccgc tgcgcgatcg
     4381 ccagtgcgag atcccgctgc tggcccgcgc gttcctcgtc gatgcctgct cgcgcagcgg
     4441 gcgagaccgg atgacgatta caccggccgc aatgcaggta ctgctcacct acagctggcc
     4501 ggggaacgtt cgcgagctgc gcaacacgat ggagtacgtg atggcggcgg cgcccgacga
     4561 caacgtcgag ccgctcgatc tgcccgagcg cctcggcggt tcggcagcgc caccgaccgc
     4621 ggctgcggtt ccgcacgatg ttgtcgccga cggtaagacg acgtttcggc caatcaacga
     4681 cgagctcaag gagctcgagc gccgccgcat ggccgaagca ctcgctgccg ccggcggcgt
     4741 gaagacgaaa gccgcacagc tgatcgacat gccgatccgc acgttcacgc tgaagctgaa
     4801 gcagtacaag ctgtgagcga ggagcggtgg ttgttgccgg gcaagttttg cctgcacggc
     4861 agcgcttgcc ggtcgcaggg gcggaagtcc tcgtcgtgat gcggacagtc ccaccgattc
     4921 gttggtccgc ttcgtgctct agcggtctgc atgcgcaact tcctcctcgt gattctcgtc
     4981 ggtgcgtcgg catgtgcggc gcgtagctcc aaccagccca cactccctgt tcactacgcc
     5041 tgcggcgaag ccaccgtcac ccgcaacggc atcgagatgc ggtcgtccgc gacgccaggc
     5101 gagatcagcc ggctgtcgtg gcacgacgac aagggcgagc acttcgtgac gtggccgctg
     5161 tcgccgaccg atcgcactgc gaccgagttc gtcgtgccga gcgatccgcg ccaggacgcg
     5221 acgcagcgca cgtacgacac gacgttcggt tcgtcgaccg ctgactggcg cctgacggac
     5281 aagcaggtct gcacggcgcg aggcggttac aatgacctgc tcgcgcgcta catgcgcggc
     5341 gagtcgatcg acgatctgac ccgcgacgtc ggcctgtcga gccgcgaaga aacccgcgcg
     5401 gtgatccgca aggcgcttgt ctcgctgcag aagaagtact ggagcgagtg agccctaccg
     5461 aggaagttgt ttgatgtagc ccgagcacgc ccgtgctcgg gcttttctgc gtcgccggtt
     5521 actcttttgc agagcggatc gtgagcacgg cgcaggggct catgcgcacg acacgctccg
     5581 caacgctgcc gacgagcaag tgctcgaggc cggtgcggcc atgcgtgccc atcgcgatca
     5641 gatcgacctt gttctcggtc gcgaacttca cgatctcgtc ctcgggcttg ccgatgcgga
     5701 ggaacgtctg cgcggcgacg ccgtctttgg cgacgcgcgc cgcgagatcc gcgaggtgcg
     5761 cctcggcttg cttgatcgca tagtccttga tgttgatcgg cgcgccgttt tcgtcgggaa
     5821 cgatcgtgat gtcgggtttg atcgctgtcg gcaggtcgat gacgtgcaac agcaacaggt
     5881 tcgcgccgtg cgctttcgct tcatcgcgtg cgatcgcgac ggcgtgattc gctgacgacg
     5941 agaaatcgtg cggaaccagg atcgtcttcc agatcatggc cgcgagcgtc gcagaggcct
     6001 acagcgcggg caagccccag gaggtagcga ctgcgttgta ctcgctgcgc ggaagcaata
     6061 ggtacagcgg ctgactcgcc ggatcgagct tctcgagcag cgcctcgagc ttcggcttct
     6121 ccatcgcggt gcagccgacc gtcgtcgatt cggggccgct ccacacgtgg aggaagatgc
     6181 agctgccggc acctggcttg gcgtcgggat tgtgggcgac gtcgagcacc cactcgtacg
     6241 cctcgtcgtc gcgacgcatc tgctcggcgg attgccagtc ggacgcgacc tgcttgcggt
     6301 cgatgatctt cgtgtagtgc tcggacgcag cgtcgtcaac gcactcgaga tcggtgctct
     6361 gctcgtacgg catgcgccag cccttcggcg cgtcgtccgc gtaaccgtag ctcgaacgaa
     6421 acgcgaatac gccggcgggg ctcttgccgt cgccttcctt cttcgtcggg ccttcgcgat
     6481 tgggcgccga cggatagaga ccgatgcccc aaccactgcc gctcttgccg atcaccgcgg
     6541 gccagtcatc gccgacgcgc tgccagccgg cctctgtgcg ttgccacagt gcgagccgcg
     6601 cgtgcgtcgt ggtccagtcg tcgacgatcg cgatgacgag ctgcttcgtt tgcttcggga
     6661 tcgcgaaacc gcgcggtgcg atcgcgttcg tcgccgatgc agccggcggt gttgccgctt
     6721 tgcgatcgca agcggtgccc aaggcaacga acgtcgcaac gatcgtagcg aggacgattg
     6781 gcgcgcgcgt cacgcgctca tctcgagcat gcgttggatc ggcctcaacg ctttgatgcg
     6841 cgtctcttcg ggaacgtcga cctgcggctg cagatcgcgc atgcagaggt acagcttctc
     6901 gagcgtgttc tttcgcatga acggacacat gttgcaggag cagctctcgt cctcgggcgg
     6961 cgcagcgatc agcgtcttgc cgggcgcggc ttgctgcatc ttgtgcatga tgccggcctc
     7021 ggtcgcgacg atgaacgtct gcttcgcgct cttcttcgcg tactcgatga ggccgctcgt
     7081 cgatgcgatg tgattcgcgt ggcgcaggat ctccgcggtg cactcgggat gcgcgatcac
     7141 ttcggcgtcg ggatgctgcg ccatcagctc gagcagccgc ttctcggaga acgtctcgtg
     7201 cacgatgcag gtgccctgcc acgtgaccag atcgggacgg ttcgcttgct tcgcgacgaa
     7261 cgcgccgagg tgacggtcgg gcgcgaacac gaccttctga ccttcgagcg actttacgat
     7321 cttcaccgcg ttcgacgacg tgcagatgat atcgctcagc gccttcacgt cggcggagca
     7381 gttgatgtag ctgacgaccg tgtggccggg atacttctcg agccactgct tgaacacggg
     7441 cgccgggcaa ccgtcggcga gcgagcagcc ggccgacaga tccggaacga cgacgatctt
     7501 gtcggggttc agaatcttcg cggtctcggc catgaaatgg acgccgcaga acacgatcac
     7561 gtcggccttt gtcttggccg ccgcctgcga tagctggagc gagtcgccga cgaagtc
//
LOCUS       DASUFX010000037         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_6353,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000037 DASUFX010000000
VERSION     DASUFX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6353"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFV99_01430"
     CDS             complement(<1..897)
                     /locus_tag="VFV99_01430"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5057983.1"
                     /translation="MLGPHVASALRRTARIAVERPRATLWTMLALTAALFAVGLAGLT
                     AIHVERWTSSPRLGASMVVYLGDGVDEAKAQGLATELAKLPGVERAELVTAADSGARL
                     QQALGADSQLLEGVELASLPASVEVTLAPGVRDVIAMSPTVRALRGTPGVDDVVVEDA
                     GADKVAGTLRTVRIVAWTGAALLAGLALIVVLAFIRVRLDRAEQELTVLHLLGASPSF
                     IIVPTALAGALQGAIAAMLAAGALAMGVSIYGDGVAAALHDALGSVQIALPAASMLVM
                     FIALGAALGLVGGGLAGASRAAR"
     gene            complement(897..1097)
                     /locus_tag="VFV99_01435"
     CDS             complement(897..1097)
                     /locus_tag="VFV99_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057984.1"
                     /translation="MRTLRKLRTLAPRDTRIRSRRFDFDVTDATMEPDPGPAPEAVFD
                     RVTGPWDRRRSFSVPGASDEET"
     gene            complement(1100..1906)
                     /locus_tag="VFV99_01440"
     CDS             complement(1100..1906)
                     /locus_tag="VFV99_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5057985.1"
                     /translation="MIELVDVRLDHPRGGEPLVSEANLKVARGEVVLIVGAAGVGTSR
                     LVAAALGEVPTNGGRIEVMGRDLTKLRRSSLRLLRRRVGIVPQDLCLLDDRSAQLNVA
                     LPLEIDGLPRSMTVVRANEVLAQLGLKSEAPLPIDCLGGAERQRVAVARALVRTPEIV
                     IADHPTSLQDAEGAELVCNALAAASKQGAAVIVLGRDQALRSIAERYQWRQLALLDGK
                     LRALAELNLADSAIDDILTGLESAPTPLHPDEDIPNVVPFPLSARTAGVA"
     gene            1994..2698
                     /locus_tag="VFV99_01445"
     CDS             1994..2698
                     /locus_tag="VFV99_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057986.1"
                     /translation="MKLYPVLSICTLVACRSNESDTVAKLETRVAAAEQRVAAIEQRG
                     PVDTRAVAEELLAKGSAAGLHGPPGSEGPAGPKGDPGPPGPAGTGPPGPIGERGPKGD
                     PGPQGPEGHQGIQGLQGPQGLQGPQGAQGPKGPVGPAGAYSGKTDLARREHRVSVGAG
                     LVATAVAGCERASDLLVTGGCYADPQWMAQLVASRAIAMTDAQNPASWRCDYKNTSPS
                     STIEIVAEAYCVRPRE"
     gene            2702..>3333
                     /locus_tag="VFV99_01450"
     CDS             2702..>3333
                     /locus_tag="VFV99_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057987.1"
                     /translation="MGAWDYGLYGNDEALNTRGVVLAKVVLPEDPRLFAGCVGLLALL
                     EPEAHQFVDIGNHPALTALPQVLREATMVAAQVTASGPRLPYTERVRDILGIDASYGR
                     VVDPLLALRETITIARSIRDRCTKLVDDGFAYGDTAIGGVVGVLLELRELGVATAPDL
                     VDEWIESFDHLRDIGEDDTDFLREWSRSYRAALKLLSTEPDKKLLGSEPT"
BASE COUNT          581 a         1138 c         1126 g          488 t
ORIGIN      
        1 gcgagccgca cggcttgctc ctgcgagccc gccaccgacg agcccgagcg ctgcgccgag
       61 cgcgatgaac atcacgagca tcgaggcggc cggcagcgcg atctgcaccg agccaagtgc
      121 gtcgtgtagt gcggccgcaa cgccatcgcc gtagatcgag acgcccatcg cgagtgcgcc
      181 cgcagcgagc atcgccgcga tcgcgccttg cagcgcgccg gccagcgcgg tcggcacgat
      241 gatgaacgaa ggcgatgccc cgagcaggtg cagcacggtg agctcttgtt cggcgcggtc
      301 gagacgaacg cggatgaacg cgagcacgac gatcagcgcg agaccggcga gcaatgccgc
      361 acccgtccac gcaacgatgc gcacggtgcg tagcgtgccc gcgaccttgt cggcacccgc
      421 atcttcgacg accacgtcgt cgacgccagg tgtgccacgc agcgcgcgaa cggtcggact
      481 catcgcgatc acgtcacgca cgcccggtgc gagcgtgact tcgacggacg ccggcaggct
      541 cgcgagctcg acgccttcga gcaactgcga atcggcgccc agtgcttgct gcaagcgagc
      601 tccagaatcg gcagcggtca cgagctccgc gcgttcgaca ccgggcagct tggccagctc
      661 ggttgcgagc ccttgggctt tggcttcgtc gacgccgtcg ccgaggtaca cgaccatgct
      721 cgcgccgagc cggggcgaag atgtccagcg ctcgacgtgg atcgcggtga gacccgcgag
      781 accgaccgca aacagcgccg cggtgagcgc gagcatcgtc cacagcgtcg cgcgcggacg
      841 ttcgaccgcg atccgcgctg tacgtcgcag cgcggacgca acgtgtggac cgagcactac
      901 gtttcctcgt cgctagcgcc gggcaccgag aacgagcgcc ggcgatccca cggaccggtg
      961 acgcgatcga agacggcctc tggcgcaggc cccggatcgg gctccatcgt cgcgtcggtc
     1021 acgtcgaagt cgaaccggcg ggaccgaatt cgagtgtcgc gcggcgcgag tgtgcgcagc
     1081 ttgcggagcg tcctcatctt catgcaacac ctgccgtgcg agcgctgagg gggaatggca
     1141 ccacgttggg aatgtcctcg tcgggatgga gcggcgtcgg cgccgactcg agcccggtca
     1201 gaatgtcgtc gatcgcggag tcggcgaggt tgagctcggc gagcgcgcgc agcttgccgt
     1261 cgagcagcgc gagctggcgc cattgataac gctcggcgat cgagcgcagc gcctgatcgc
     1321 ggccgagcac gatcacggca gcgccctgct tgctcgccgc ggcgagcgcg ttgcacacga
     1381 gctcggcgcc ttcggcgtcc tgcaggctcg tcgggtgatc ggcgatcacg atctccgggg
     1441 tgcgcacgag agcgcgcgcg actgcgacgc gttggcgctc ggcaccgccg aggcaatcga
     1501 tcggcagcgg tgcttcggat ttgaggccga gctgcgcgag gacttcgttc gcgcgaacga
     1561 ccgtcatcga gcgcggcaag ccatcgattt ctagcggaag cgcaacattg agctgcgcgc
     1621 tgcgatcatc gagcaggcag agatcctgcg gcacgatacc gacgcgtcgc cgcaacaggc
     1681 ggagggacga cctgcgcaat ttcgtcagat cgcggcccat cacctcgatg cgaccaccgt
     1741 tcgtcggaac ttcaccgagc gccgcagcga cgaggcggga cgtaccaacg cccgctgcgc
     1801 cgacgatcag caccacttca ccgcgcgcga ctttgaggtt cgcttcgctc accagcggct
     1861 ctccaccgcg tgggtggtcg agtcgaacat cgaccagctc gatcatgcga ccagctaagg
     1921 tcattcgatc gacggaggca aaaaaacggc cgaatcgccc gctccacgtg catcatttcg
     1981 taggattgcc gccatgaagt tgtacccggt cctctctatc tgcacgctcg tcgcgtgtag
     2041 gtctaacgag agcgacacgg tggcaaagct cgagacccgc gttgccgctg ccgagcaacg
     2101 tgtcgctgcg atcgaacagc gcggccccgt cgatacgcgc gccgtcgccg aggagctcct
     2161 cgcgaaaggc agcgccgcgg gcctgcacgg accgccggga tccgaagggc ccgcaggtcc
     2221 gaaaggcgat ccgggtccgc cgggtcccgc gggcacgggg cctcccggac cgatcggaga
     2281 acgcggaccg aaaggcgatc caggaccgca aggtcccgaa ggacaccagg gcattcaagg
     2341 cctgcaagga ccacagggcc tgcaagggcc gcagggcgcg caaggcccca agggtccggt
     2401 cggcccggcc ggcgcgtaca gcggcaagac ggatctcgcg cgtcgcgagc atcgcgtgtc
     2461 ggtcggcgcg ggactcgtcg cgacggcggt tgccggatgc gaacgcgcgt ctgacttgct
     2521 cgtcaccggc ggttgctacg ccgatccgca gtggatggcg cagctcgtcg cctcgcgcgc
     2581 gatcgcgatg accgacgcgc agaatcccgc gtcatggcgc tgcgactaca agaacacatc
     2641 cccgagctcg acgatcgaga tcgtcgccga ggcctactgc gtgcggccac gcgagtaact
     2701 catgggcgcc tgggattacg gactctacgg caacgacgaa gcgctcaaca cgcgcggtgt
     2761 cgtgctcgcg aaggtcgtgc tgcctgaaga tccgcgcctg ttcgccggct gcgtcggctt
     2821 gcttgcattg ctcgagcccg aagcacacca gttcgttgat atcggcaacc accccgcact
     2881 cacggcgttg cctcaagtct tgcgcgaagc gacgatggtc gctgcccagg taaccgcgag
     2941 cggcccacgg ctgccgtaca ccgagcgcgt gcgcgacatc ctcggcatcg acgcgagcta
     3001 cggccgtgtc gtcgatccac tgctcgcgct gcgcgagacg atcacgatcg cgcgttcgat
     3061 tcgcgatcgc tgcacgaagc tcgtcgacga cggcttcgcg tacggcgata ccgcgatcgg
     3121 cggcgtcgtc ggtgtcttgc tcgagctgcg cgagctcggc gtcgcgaccg cgccggatct
     3181 cgtcgacgaa tggatcgaga gcttcgatca tctgcgcgac atcggcgagg acgacaccga
     3241 cttcttgcgc gaatggtcgc gctcgtatcg cgccgccctc aagctactca gcaccgagcc
     3301 cgacaagaag ctgctcggca gcgagccgac gat
//
LOCUS       DASUFX010000038         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_700843,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000038 DASUFX010000000
VERSION     DASUFX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700843"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2249)
                     /locus_tag="VFV99_01455"
     CDS             complement(<1..2249)
                     /locus_tag="VFV99_01455"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5057988.1"
                     /translation="MRRFSLLASIVLSAACAHTQHHISVEGAVLGRVVIYRNGVAFYE
                     RTAKVTDGHVSVRVPRDRVDDFLKSLTVVDPVTRKPLAVSIPRQEADDGSYLTMTLET
                     ADRKYAEVLLTYVTESPAWKPSYRVVVGGNNKVMLEGWAIVDNVSDEDWKGVLVGVGA
                     SSALSFRYDLWSVRRIDRDLLQGEDRFAIAPPTGVSPYAETTGAEELVTLDANEVRSV
                     PGGARVAGSQGDAYGVAFSGSSSLENQYYVDGINTTGLTNSPDAPASSSSSSLPPAIA
                     SGVRGVVSDKKTGEKLAGVTVIATKKGGKETLTAITDESGAYNITAPAGTYDLAYFYG
                     DVTIERKGINIANNQVTPVYQKLDGGMVGGETIAITDRAPTIDPTSTTQGITITKDYL
                     RNIPVPGRTFDSALGSAAGSSGGSYKPPPPVKQGDAKLSGIVKKVLDSKKDVVVETHG
                     LSSAEVSKRAEAVKNKLIDDGIPSTRIHIVPKIGPNESISLRVLAVAPTGNAEKNSAP
                     PSAAARAAMPDTPVGESHFMADRPMNVRAGSSAMVAMVHGETTGGVVYLYDPISDRGD
                     ERFAFKAVRLDNPKDDTLEPGPVTVYGDGRFIGEGITEPVPPKASVVVPFALDRQVLI
                     SRNNSENDRIAKLVTVQRGVVTAEVQHRRQTRFTVTSRLTQPTKVYLRHRLESGWALV
                     EAPSKYMKVGDSQLFEVDLAAGETKYVTIAEATPVQRSLELGSAEALDMMKVFIDEPD
                     ASPELKKQI"
     gene            complement(2357..>3339)
                     /locus_tag="VFV99_01460"
     CDS             complement(2357..>3339)
                     /locus_tag="VFV99_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5057989.1"
                     /translation="ARMMALQRQGRIGFYGEAMGQEAAVVGSAAATEPQDWIVPALRE
                     AGVGLFRGMTLDSYIAQIFGNAADVTKGRQMPCHPCDRDRHYVVMSSCVSTQVPHAVG
                     IAMGMKIGGDRGKVCFGYMGDGGTSEPDMHVALNFAGVMKAPVVLICQNNQWAISTPG
                     TMQTAADTIALKGIGYGVEALRADGNDAFAVYEVVKYAADKARRGDGPTFIELLTYRV
                     SAHSSSDDPTRYRDEKVTEVWRHQRDPLRRMETYLTKRGWLQTGEREALAAQLETDVR
                     DAIERQEAIGTPELSTLVEDVFEEPTWNLREQMAELEAAERQKSPHKHGG"
BASE COUNT          496 a         1135 c         1054 g          654 t
ORIGIN      
        1 ttgatctgct tcttgagctc gggcgatgca tccggctcgt cgatgaacac cttcatcatg
       61 tcgagtgcct cggcggagcc gagctccagc gaacgctgca ccggcgtggc ctcggcgatc
      121 gtgacgtact tcgtctcgcc ggccgcgagg tcgacctcga acagctgcga atcgccgacc
      181 ttcatgtact tcgacggcgc ctcgacgagc gcccagccgc tttccaggcg gtggcgcaag
      241 tagacctttg tcggttgcgt gagtcggctc gtgaccgtga accgcgtctg gcggcgatgc
      301 tggacttcgg cggtgaccac gccgcgctga acggtgacga gcttcgcgat ccgatcgttc
      361 tcgctgttgt tgcgcgagat cagtacttgg cgatcgagcg cgaacggcac gaccaccgac
      421 gctttcggcg gcaccggctc ggtgatgccc tcgccgatga agcgcccgtc gccgtacacc
      481 gtcacggggc cgggctcgag cgtgtcgtcc ttcgggttgt cgaggcgcac tgctttgaag
      541 gcaaagcgct cgtcaccgcg atcgctgatc gggtcgtaga gatagacgac gccacccgtc
      601 gtctcgccgt ggaccatcgc gaccatcgcg ctcgaacctg cgcgcacgtt catcgggcgg
      661 tccgccatga agtggctctc gccgaccggc gtgtcgggca tcgcggcgcg tgcagccgcg
      721 cttggcggcg cgctgttctt ctcggcattg ccggtcggcg cgaccgccag cacacgcaac
      781 gagatgctct cgttcggccc gatcttcggc acgatgtgga tacgcgtcga tgggatgccg
      841 tcatcgatga gcttgttttt caccgcttca gcgcgcttgc tgacctccgc gctcgatagc
      901 ccgtgcgtct cgacgacgac gtccttcttg ctgtcgagca ccttcttgac gatgccagag
      961 agcttcgcgt cgccttgctt caccggcggc ggcggtttgt agcttccacc cgatgatccc
     1021 gcggcactgc cgagtgcgct gtcgaacgtg cgtcccggca cggggatgtt gcgcaggtag
     1081 tccttcgtga tcgtgatgcc ctgtgtcgtc gacgtcggat cgatcgtcgg tgcacggtcg
     1141 gtgatcgcga tcgtctcgcc gcccaccatg ccgccgtcga gcttctgata gaccggcgtc
     1201 acctggttgt tcgcaatgtt gatgcctttg cgctcgatgg tgacgtcgcc gtagaagtag
     1261 gcgaggtcat aggtgccggc gggcgcagtg atgttgtacg cgccgctctc gtcggtgatc
     1321 gcggtgagcg tttccttgcc gcctttcttc gtcgcgatca cggtgacacc ggcgagcttc
     1381 tcgccggttt tcttgtcgct gactacgcca cgtacgccgc tcgcgatcgc cggtggcaac
     1441 gagctgctgg acgagctcgc cggggcgtcg gggctgttcg tcaggcccgt cgtgttgatg
     1501 ccgtcgacgt aatactggtt ctcgaggctc gacgagcccg agaacgccac gccatacgcg
     1561 tcgccctgtg agccggcgac ccttgcaccg cccggcacgc tgcggacctc gttggcatcg
     1621 agcgtcacga gctcctcggc gcccgtcgtc tccgcgtacg gcgacacacc ggtcggcggt
     1681 gcgatcgcga atcgatcttc gccctgcaac aggtcgcggt cgattcgccg cacgctccac
     1741 aggtcgtacc ggaacgacag cgccgagctc gcgccgacgc cgacgagcac gcccttccaa
     1801 tcctcgtcgg acacgttgtc gacgatcgcc cagccctcga gcatcacctt gttgttgccg
     1861 ccgaccacga cgcgatagct cggcttccat gccggcgatt cggtgacgta cgtcagcagc
     1921 acctcggcgt acttccggtc ggccgtctcc agcgtcatcg ttagataact gccgtcatca
     1981 gcctcctgtc gcgggatcga gaccgcgagt ggcttgcgcg tcaccgggtc gacgacagtc
     2041 agcgacttga ggaagtcatc gacgcggtcg cggggtactc ggaccgacac gtggccatcc
     2101 gtgaccttgg cagtgcgctc gtagaacgcg acgccattgc gatagatgac cacgcgcccg
     2161 agcaccgcgc cctcgacgga gatgtggtgc tgcgtgtggg cacaggcggc ggacaacacg
     2221 atgctggcaa gtagagagaa gcgacgcatg cttcttcact acgcgtcgca ctgcgaccgt
     2281 tccacgagac cctgctgacg ccttcgctct tgctgcggtg tcgcaccctg cgagcttgtc
     2341 gcaggtcagc gcgacatcag ccgccgtgct tgtgcggcga cttctggcgc tctgccgcct
     2401 cgagctcggc catctgctcg cgcagattcc acgtcggctc ctcgaacacg tcttcgacga
     2461 gtgtcgacag ctccggcgtg ccgatcgctt cctggcgctc gatcgcgtcg cgcacgtccg
     2521 tttcgagctg cgccgccagg gcctcacgct cgccggtctg tagccagccg cgctttgtca
     2581 ggtacgtctc catccgccgc agcggatcgc gctggtgacg ccacacttcg gtgaccttct
     2641 cgtcgcgata acgcgtcgga tcgtccgagc tggagtgcgc gctcacgcga taggtgagca
     2701 gctcgatgaa cgtcgggccg tcgccgcgcc gcgccttgtc ggccgcgtac ttcaccacct
     2761 cgtagaccgc gaatgcatcg ttaccgtcgg cgcgcagcgc ttcgacgcca tagccgatgc
     2821 ctttgagcgc gatcgtatcg gcggcggttt gcatggtgcc cggcgtcgaa atcgcccact
     2881 ggttgttctg gcaaatgaga acgacaggag ccttcatgac gccggcgaag tttagcgcaa
     2941 cgtgcatgtc gggctccgac gtgccgccat cgcccatgta tccgaagcac accttgccgc
     3001 gatcgccgcc gatcttcatg cccatcgcga tgccgaccgc atgcggcacc tgcgtcgaca
     3061 cgcacgacga catcacgacg tagtgacggt cgcgatcgca cggatgacag ggcatctgcc
     3121 ggcccttcgt cacgtccgcg gcgttgccga agatctgcgc gatgtagctg tcgagcgtca
     3181 tgccgcggaa caaaccgacg ccggcttcgc gcagcgcggg cacgatccaa tcttgcggct
     3241 cggtcgcggc tgccgagccg acgaccgcgg cctcctgacc catcgcttcg ccatagaaac
     3301 cgatgcgacc ctggcgttgc agcgccatca tgcgcgcgt
//
LOCUS       DASUFX010000039         4931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_6557,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000039 DASUFX010000000
VERSION     DASUFX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6557"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="ttcA"
                     /locus_tag="VFV99_01465"
     CDS             complement(<1..658)
                     /gene="ttcA"
                     /locus_tag="VFV99_01465"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX5057990.1"
                     /translation="MSEVVRLEKLLYRTVKETCEGYELLRPGDRIMVAMSGGKDSYTL
                     FHLLTKLVPRLPFKIELVAVHLDQVQPNYDGTGLRGYLEATGGPFEILREDTYSVVTT
                     HIDDKSTYCSLCSRLRRGILYTAAERLGCNKLALGHHRDDSLETFLLNLFYSGKLQAM
                     PASYRTDDGRFEVIRPLIGCAEADIAELASHLGFPIIPCNLCGSQDGLKRDAMTALIA
                     E"
     gene            657..1418
                     /locus_tag="VFV99_01470"
     CDS             657..1418
                     /locus_tag="VFV99_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX5057991.1"
                     /translation="MDAFVLDPRLLVILGVVALTSFATEGAIGFGGTVLAVSLGAALA
                     IPLALLLPAFVPLNLLMSAWLLSRGFSVVGWRMLAREVAPAVGIGTAIGLALFHLPAK
                     QLFAFGFGVFVIGLATLQLVRPAEKDLPRALRWLLLGLGGVVHGLFGTGGPMIVYVAR
                     RRLPDKRAFRSTLAVLWIVLNIALLTNYASEHMYTVTTFKISGVIALMIVPGLFVGDR
                     IHHKLDAARFERVVWVVLLFAGFAVAIRNAHALYG"
     gene            complement(1409..3238)
                     /locus_tag="VFV99_01475"
     CDS             complement(1409..3238)
                     /locus_tag="VFV99_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057992.1"
                     /translation="MRAAAVLLLAACGGHGASPDARVDTGDGPPIPDAHEWACAPGTG
                     MPAKVCSVDHLCWEEPSPQGLDFGPADGKSCDYWIIGGGGKVYRLNGDTLTQMAEQPG
                     QYGGVSALKVFSDVDAWAVSSNGAFHWTGGTWIQISTDGFYGVWGSSPIDVWLSGKNG
                     MMSHWDGMSLTTMSTPATLQLGVTYGTASNDVWMLALGNEAVHWNGSQWTSTSFGTNQ
                     LERSDLWGANPSTYFAVGSTYPKPQIWRYNGADWMLEHEEAAPPAPLYYASYDAVWGT
                     SANNVWVGGMNLDHVVHYDGANWTNVPLPSAGVQIRSIRGSGPNDVLFMGLRGYLARW
                     NGSEIVELSRDTNGPSWRRIWASAANDIWILGDDNTIRRYNGTTWTAQSSPTIFVWGL
                     SGVSGDDVWAAGTLSQHMQVAHWNGAAWTATDLGVSGSLRAIHVAAANDAWAVGDNGK
                     TFHYNGTWSQVTSPATLRLGAIYGDATEAWAGGDTNTLQHWQNNTWTTVTSPATTDYW
                     DAFGRGPNDVYLSGFLGTVVHYDGNTWTKLTTGTNQLLYDVGGDATRVWFAGSGGAVG
                     LDSGSGFKLLEAGTKQYLNGAHFTSATDGWVIGEYGALLRYQP"
     gene            complement(3249..4190)
                     /locus_tag="VFV99_01480"
     CDS             complement(3249..4190)
                     /locus_tag="VFV99_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057993.1"
                     /translation="MRLALLVLLIAAPAYADQAKPAVSDTRCATPPTVTVVGWRHKRS
                     KLIAKTASPNHRGIDLIANEDDATQVIEGKLAYGKLDKDVDKEDAELFACIDNAWRSL
                     GVRTTDDDGRLALTLEGKARLPVGMRDMYVAAVGDGSGAYFVGYVAPRGTKVVITDVD
                     GTISWSENSIIKTVVSRDHDIKHRPNAPEALAALPYPVIYTTARGDVFIDVTRKWLDR
                     HGFPRGLLRLSKGMFAKPGESAIEYKTKTLKSISVPIAAGIGNRKSDITAYTAIGLTA
                     KQIFIHLPEYEKELRKDLAAGNAVGFKDYLQLPKLLP"
     gene            complement(4195..>4931)
                     /locus_tag="VFV99_01485"
     CDS             complement(4195..>4931)
                     /locus_tag="VFV99_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX5057994.1"
                     /translation="FELVRRGALPSADLPHYDHIFIDEAQLRSPLELAAVGDALAKGS
                     TVTLAGDHRQATDESAWFTGWTGARSELRAQRWSEITLAITYRSVPAIGDFARAVSEG
                     NAALPSEPPVNPALWATACDGPLAQAAALCWHLDALITRDPWRQIAVIARNPDHARRL
                     HAELSRGVDPTLVLDGDFRFEPGIIVTTAAAVSGLEFDSVVVPDLSSSFYPSDDLRRA
                     LYVVATRARDWLWLLTPERWSPLVQQ"
BASE COUNT          765 a         1651 c         1609 g          906 t
ORIGIN      
        1 gctcggcgat cagcgcggtc atcgcatcgc gcttgagccc gtcctgcgag ccgcacaggt
       61 tgcacggaat gatcggaaac ccgaggtgcg acgcgagctc cgcgatgtct gcttcggcgc
      121 agccgatcag cggccggatc acttcgaagc ggccgtcgtc agtgcgatag ctcgccggca
      181 tcgcttgcag cttgccgctg taaaacagat tgagcaggaa tgtctcgagt gaatcgtcgc
      241 ggtgatggcc gagcgcgagc ttgttgcaac cgaggcgctc ggcggcggtg tagaggatcc
      301 cgcgtcgcaa ccgcgagcac agcgagcagt acgtcgactt gtcatcgatg tgcgtggtga
      361 cgaccgagta ggtgtcctcg cgcaggatct cgaacggccc gccggtcgcc tcgaggtagc
      421 cgcgcaggcc ggtgccgtcg tagttcggct gcacctggtc gaggtgcacc gcgacgagct
      481 cgatcttgaa cggcaaccgc ggcaccagct tggtcagcag gtggaacagc gtgtagctgt
      541 ccttgccgcc cgacatggcg accatgatgc gatcgccggg acgcagtagc tcgtagccct
      601 cgcacgtctc cttgacggtg cgatacaaca gcttctcgag ccgtacgacc tcggacatgg
      661 acgcattcgt actcgatccg cgcctgctcg tcatccttgg agtggtggcg ctcacctcgt
      721 tcgccaccga gggcgcgatc ggctttggcg gcacggtgtt agccgtgagc ctgggcgccg
      781 cgctggcgat tccgctcgcg ctgctgctgc cggcgttcgt cccgctgaat cttctgatgt
      841 ctgcgtggct gctcagccgc ggcttttccg tggtcggctg gcggatgctg gcgcgcgagg
      901 tcgcgccggc ggttggtatc ggcaccgcga tcggtctcgc ccttttccac ctgcccgcga
      961 aacagctgtt cgcgttcgga ttcggcgtct tcgtgatcgg cctggccacg ctccagctcg
     1021 tacgtcccgc cgagaaggat ctgccgcgcg cgcttcgctg gctactgctc ggcctcggcg
     1081 gcgtcgtcca cggtctgttc ggcaccggcg gtccgatgat cgtctacgtc gcgcggcggc
     1141 gcctgcccga caagcgcgcg tttcgctcga cgctcgccgt actgtggatc gtgctcaaca
     1201 tcgcgctgct gacgaactac gcatcggagc acatgtacac ggtcacgacg ttcaagatct
     1261 caggcgtgat cgcattgatg atcgttcccg gcctcttcgt tggcgatcga attcaccaca
     1321 agctcgacgc ggcgcggttc gagcgtgtcg tgtgggtcgt gttgctcttc gccggtttcg
     1381 cggtcgcgat ccgcaacgcg cacgcgctct acggctgata gcgcaggagc gcgccatact
     1441 cgccgatgac ccagccatcg gtcgccgacg tgaagtgcgc gccgttcaaa tactgtttcg
     1501 tgcccgcctc gagcagcttg aagccgctgc cgctgtcgag gccgaccgcg ccgcccgagc
     1561 ctgcgaacca cacgcgcgtc gcgtcaccgc cgacgtcgta gagcagctgg ttcgttcccg
     1621 ttgtcagctt cgtccacgtg ttgccgtcgt agtgcacgac ggtcccgagg aagccggaga
     1681 ggtagacgtc gttcggaccg cgcccgaacg cgtcccagta gtcggtcgtc gccggtgacg
     1741 tcacggtcgt ccacgtgttg ttctgccagt gctgcagcgt attcgtgtca ccgcctgccc
     1801 acgcctcggt cgcatcgccg tagatcgcgc cgagccgcag cgtcgccggg ctggtcacct
     1861 ggctccacgt gccgttgtag tggaacgtct tgccgttgtc gccgacggcc caggcgtcgt
     1921 tcgccgccgc gacgtggatc gcacgcaacg aaccgctgac gccgagatcg gtcgctgtcc
     1981 acgcggcgcc gttccagtgg gcgacctgca tgtgctgcga gagcgtgccc gccgcccaca
     2041 cgtcgtcgcc gctgacccca gagagtcccc acacgaagat cgtcggtgac gactgcgccg
     2101 tccacgtggt gccgttgtag cgacggatcg tgttgtcgtc gccaaggatc cagatgtcgt
     2161 tcgccgcgct cgcccagatg cgccgccacg atggcccgtt ggtgtcgcgc gacagctcga
     2221 cgatctcgct accgttccag cgcgcgagat aaccgcgcag gcccatgaac aggacgtcgt
     2281 tcggcccgct gccgcgaatc gaccgaatct gtacgccggc gctcggcagc ggcacgttcg
     2341 tccagttcgc gccatcgtag tgcacgacgt ggtcgaggtt catgccgccg acccacacgt
     2401 tgttggcaga tgtaccccac accgcgtcgt agctcgcgta gtagagcggc gccggcggcg
     2461 cagcctcctc gtgctcgagc atccagtcgg cgccgttgta gcgccagatc tgcggcttcg
     2521 gataggtact gccgaccgcg aagtaggtgc ttggattcgc gccccacaga tcactgcgct
     2581 cgagctggtt cgtgccgaac gaagtgctcg tccactgcga gccgttccag tgcaccgcct
     2641 cgttgccgag cgcgagcatc cacacgtcgt tcgacgcagt gccgtacgtg accccgagct
     2701 gaagtgtcgc cggtgtcgac attgtcgtca gcgacatgcc gtcccagtgc gacatcattc
     2761 cgttcttccc cgatagccac acgtcgatcg gcgagcttcc ccacacgccg tagaaaccgt
     2821 cggtcgagat ttggatccac gtcccgccag tccaatgaaa tgcgccattg ctgctgaccg
     2881 cccacgcatc gacgtcggag aacaccttca gcgccgacac gccgccgtac tgcccgggct
     2941 gctcggccat ctgcgtgagc gtgtcgccgt tgaggcgata caccttcccg ccgccaccga
     3001 tgatccagta gtcgcagctc ttgccgtcgg ccggtccgaa atcgagtccc tgcggcgacg
     3061 gctcctccca acacagatga tcgaccgagc acaccttcgc gggcatgccg gtgccggggg
     3121 cgcacgccca ctcgtgggcg tcgggaatcg gtggaccatc gcccgtatcg acgcgtgcgt
     3181 cgggcgatgc accatgccca ccgcacgcgg ccagaagcaa caccgcagct gcccgcatca
     3241 ggtacagtct acggcaagag cttcggcagc tgaaggtagt ccttgaagcc aaccgcgttg
     3301 ccggcggcga ggtccttgcg gagctccttc tcgtactcgg gtaggtggat gaagatctgt
     3361 ttcgctgtga gtccgatcgc ggtgtaggcg gtgatgtcgc tcttgcgatt accgatgccg
     3421 gcggcgatcg gcaccgagat cgacttgagt gtcttcgtct tgtactcgat cgccgattcg
     3481 cccggcttgg cgaacatccc cttcgagagt ctcagcaggc cgcgcgggaa gccgtgacgg
     3541 tcgagccact tgcgcgtgac gtcgatgaac acgtcgccgc gcgccgtcgt gtagatcacg
     3601 ggatatggca gcgccgccaa tgcctcgggc gcgttcggtc gatgcttgat gtcgtggtcg
     3661 cgcgacacga cggtcttgat gatcgagttc tcgctccacg agatcgtgcc gtcgacgtcg
     3721 gtgatgacga ccttcgtgcc ccgcggcgcg acatagccga cgaagtacgc gccactgccg
     3781 tcaccaacgg cggcgacgta catgtcgcgc atgccgacgg gcaaccgcgc cttgccttcg
     3841 agcgtgagcg ccagccggcc atcgtcgtcg gtcgtgcgga cgccgagcga cctccacgcg
     3901 ttgtcgatac atgcgaacag ctcggcgtct tccttgtcga cgtctttgtc gagcttgccg
     3961 taggcgagct tgccctcgat gacctgcgtc gcgtcgtctt cgttggcgat cagatcgatg
     4021 ccccggtgat tcggcgacgc cgtcttcgcg atcagcttgc tgcgcttgtg gcgccagccg
     4081 accacggtga ccgtcggcgg cgtcgcgcac cgggtgtcgc tgaccgcagg cttcgcctga
     4141 tcggcgtagg ccggagccgc gatcagcaag acgagcagcg cgagacgcac gactctattg
     4201 ttgcacgagt ggggaccacc gctcgggcgt gagtaaccac agccagtcgc gtgcacgagt
     4261 tgcaacgaca tacagagccc tgcggaggtc atccgatgga tagaaagacg aggaaagatc
     4321 tggcacgacg acgctgtcga actcgaggcc ggacactgcg gcggctgtcg tcacgatgat
     4381 gcccggctcg aagcgaaaat cgccgtcgag gacaagcgtc gggtcgacgc cgcgcgacag
     4441 ctccgcgtgc agccgtcgcg cgtgatcagg gttgcgcgcg atcacggcga tctggcgcca
     4501 cggatcgcgc gtgatcagcg cgtcgagatg ccagcacaga gctgcggcct gtgcgagcgg
     4561 gccgtcgcac gcggttgccc acagcgccgg gttcaccggc ggctcgcttg gcaacgcagc
     4621 attgccctcg gagaccgcgc gcgcgaagtc accgatcgcg gggaccgagc ggtacgtgat
     4681 cgcgagcgtg atctcggacc accgttgcgc gcgcagctcg ctgcgagcgc ctgtccagcc
     4741 ggtgaaccac gcgctctcgt cggtggcctg gcgatgatcg ccggcgagcg tgaccgtgct
     4801 gcccttcgcg agcgcgtcgc cgactgctgc gagctcgagc ggtgaacgca gctgcgcctc
     4861 gtcgatgaag atgtgatcgt agtgcggcag gtccgcgctc ggcagagcac cgcggcgcac
     4921 gagctcgaac a
//
LOCUS       DASUFX010000040         7486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_284547,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000040 DASUFX010000000
VERSION     DASUFX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284547"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFV99_01490"
     CDS             <1..1198
                     /locus_tag="VFV99_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057995.1"
                     /translation="VSSLWTGLVIAPAGLWLAKLWFAAWVECIAARLDRRMVEGAPSA
                     ENPWSREVADYLMGYVRRTGWDLDPALLRQVVFLPGKNVHGDVVAYGGGVTHVRVVID
                     RELLVMAMGPLIEGKADEKPVVWPDWTLARVGPAGRREATYEARPRKQKPSYPGMQRK
                     PLGQPATLLGYVLPAPGELVPLISDNPQDLAIVRKLLSEHYPWFAPDPDEEFDATDPT
                     DKDLLFGALVHALGMVRRHDTQLATIKLALGKYAARIASPIRARLADAYATLNFARHH
                     LIQFLHYRWQRNVDVLTARARPVRLAAVSKELLKDVVDAKKPPPSALRARLVWLSQFF
                     ADPIVDRRAVIIRRVAAGVVAAALLAVLGIAVQRSIAYHPVFVDRMQAQERALQKGPA
                     DGEAQR"
     gene            1179..2003
                     /locus_tag="VFV99_01495"
     CDS             1179..2003
                     /locus_tag="VFV99_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5057996.1"
                     /translation="MAKPKDKQNESSMLFMPTVTKATLAINKEAAGAGVLGLVGFAAL
                     FVVAIVFFVTENYFVSMVIMSFALLVYVTGAELAKLFLTSFTIFFSSKHVIPAAAYLQ
                     DTLVPLRNILMIRRDGEGNVKAGPLVEGMTVKLPDNPLVRDIQTLLEKNPDLDYAEYI
                     AHEYYVQCHEVYDHSSSHLEFVATAMPMFGLIATIIGLIGMFEGLGAEVTVEFLSPQL
                     ALALKCTLYGVLLAALYKIIASRFDQRLKALDYDFETFCRALKVLIDNKAVIEVRS"
     gene            2000..2596
                     /locus_tag="VFV99_01500"
     CDS             2000..2596
                     /locus_tag="VFV99_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5057997.1"
                     /translation="MKYVRFKSKPKFDIWPVVYIDLMTQIMMFFVILWSISQMQAKKK
                     GVTTAVGDTTVHEVTLPGDVLFPSGKTKLTPQGEEVFAKLFKDDTGEVLTFETGGLTK
                     RVLVIHGHTDSDGKKDENYVLGFMRAMTVYKEIQKYGPEIVDHAVLCTHADNSPAKEV
                     PLFTGTLTPAQSQALSEAKTKNRRITIEDQVNSVVKEE"
     gene            2593..4203
                     /locus_tag="VFV99_01505"
     CDS             2593..4203
                     /locus_tag="VFV99_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057998.1"
                     /translation="MRALGARSVTAIVLLAGTVSAAWLLERFRADYWAQLSPWVTFLA
                     GAFAFAAGTLVFVYWSLGERSRSYLRFGEKLPFGEETSLLGVRTRTLLLGHAPYVEVV
                     DVPARAMHALYIAIFFGVALISLDNRAVSLVRDVPNRFDRSSIDYCKPPAPPKQVKKQ
                     DQGCKLVERAYKLGYAKSLGSCAPGHDEERQVQRLCTRRQLDEPLLHYAWRRLDNQID
                     DLRTIDEGPGFWELFTRQLDHLETIVETTANTVAMRPRSSHHVFTNLPDPRPRLRDRV
                     GRMLERGCGARLAHLQHFPPMPAGPQGASLMFEQVLDQLMFNPIYKPIVAQCEEIVVH
                     WSAPANACEQIQANAEKALDNLGALGSVRDVLAWRDGRAELHGFDAKHALDVATPSRI
                     VSFQCLMFDDAAEPAPVAEHSFALDGVTLAVRETRTKPLTADGASQIRLYKQLAALLT
                     PGFGYGRLTSNQAIGAVPEEAAMAESFRQPTFMLTRLDLLRDADLFLGNEWLAKRPDL
                     LAVYPYHLHLKNFIEIFRRQYKEHRGRL"
     gene            4200..4862
                     /locus_tag="VFV99_01510"
     CDS             4200..4862
                     /locus_tag="VFV99_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5057999.1"
                     /translation="MKRALVKLALCSVALCGVAPVGYAEPAQDRSGYIAELIDAIRAT
                     DHAALANTRKYIQIVERNKCQAPEMTLRVGCMLEAAAQNCKQLPAGKRTRVTDPSGPA
                     SGPEREACQRASDVIAANQLSERLFVPNDVRYAIMSKQRDARTAIARELHHRYAQLVT
                     ELAMSKYFPGPRADSKALGTGIDGFCTDVSGTRDLSWQYCVAAIVWFVATDGAEPEGT
                     SR"
     gene            4859..6007
                     /locus_tag="VFV99_01515"
     CDS             4859..6007
                     /locus_tag="VFV99_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5058000.1"
                     /translation="MTKAHMAMLAAENKKLEQAGLYRPETIHPGHVVDFTTQDYLGLA
                     RHERVIAAARAALDKYGLGLGSARPLTGTREIHRELEQSVARFLQVPDAIVLGSGYLA
                     NLGFYEAMFDSRDCLFCDALVHPSTAEGVRLSSATAFPFRNNDVDDLEDKLRRSRSAR
                     FRAIVTDGVFPFDGRVAELDGICALAEKYDAMVVLDDSLGVGVLGETGRGTRELRGVM
                     QRIDVITGTFGKALGGAGGGYIAGKREVIGWLRQKAMPYLFSTALSPPLAAAAHAAIE
                     LLERGEAPLPTLRMRVAMMKKELEIRGYKILGEHPSLAIQVGGVVVLQKLVNQLYERG
                     IHVNGLCYPVVPEGQARIRLELSARHSDEDMTRAINAFDLAGRALGVI"
     gene            6007..>7486
                     /locus_tag="VFV99_01520"
     CDS             6007..>7486
                     /locus_tag="VFV99_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5058001.1"
                     /translation="MIELRCTLDRTRIAPDVATEVGCQLELVPSQGAAAAGSVALTTN
                     LCLVLDCSASMLGAKVEAAISAAKLIVSSIDARHRISVVGFATSHWVVVDNASPAETG
                     RDVINKEIERLRTMIKGSTNLGEGIRRGIKTVQRQQADASVLVVLTDGVADSQADAEL
                     ASAEANEAGVQVFAVGIGESFRADDLLKVVAPSGGTLLGERAADRLETAFAELLKRIE
                     SFVATGVQLTISPAAGVVLGATYKTSPERMLIGDNVERVIVGNLERGIAYSFHVALTP
                     PCAGSGEAEIARATLRYDVPSRGHRGAMTEISIRIAYDARAEAANPEVVRAHARAQLA
                     ALVKQLADSETRGDRTKSAELLTLLAERSAAHGNTRLVEALRGLASPIPQGELNALVL
                     AATARDVVEEPRAPEPAAVPETNRPRRTLPTPTRPSAAARPPAENVRPPSPPQPTPAP
                     PPQSTPAPPPQSTPAPPPQSTPAPPPQSTPAPPPQSTPAPPPQ"
BASE COUNT         1301 a         2550 c         2429 g         1206 t
ORIGIN      
        1 ggtctcgtcg ctgtggaccg ggctcgtgat cgcacccgcg gggttgtggc tcgcgaagct
       61 ctggttcgct gcgtgggtcg agtgcatcgc cgcgcggctc gatcgccgca tggtcgaagg
      121 cgctccgtcc gccgaaaatc cgtggtcgcg cgaggtcgcc gattacctca tgggctacgt
      181 gcgccgcacc ggctgggatc tcgatcccgc gctgctgcgt caggtggtgt tcttgccggg
      241 caagaacgtg cacggtgacg tcgtcgcgta cggcggcggc gtcacgcacg tgcgcgtcgt
      301 catcgatcgc gagctactcg tgatggcgat gggcccactc atcgaaggca aggccgacga
      361 gaagccggtc gtgtggcccg actggacgct cgcgcgagtg ggtccggcgg ggcgcagaga
      421 agcgacgtac gaggcgcgcc cacgcaaaca gaagccgagc tacccgggca tgcagcgcaa
      481 accgctcggg cagcctgcga cgctgctcgg ctacgtgctc cccgcgccag gcgagctcgt
      541 gccgctgatc tcggataacc cgcaggacct cgcgatcgtc cgcaagctcc tgtccgagca
      601 ctacccgtgg ttcgcgccgg atcccgacga ggaattcgac gcgaccgatc cgaccgataa
      661 ggatctgctg ttcggcgcgc ttgtccacgc gctcggcatg gtgcggcgcc atgacacgca
      721 gctcgcgacg atcaagctcg cgctcggcaa gtacgccgcg cggatcgcgt cgccgatccg
      781 cgctcgcctc gccgatgcct acgcgacgct caacttcgct cgccatcatc tgatccagtt
      841 cctccactac cgctggcagc gcaatgtcga cgtcctcact gcacgcgcgc gcccggtgcg
      901 actcgcggcg gtgtctaagg aactgctgaa ggacgtcgtc gatgcgaaga agccgccgcc
      961 aagtgcgctg cgcgcgcgtc tcgtgtggct gtctcagttc ttcgccgatc cgatcgtcga
     1021 tcgtcgagcc gtgatcatcc gacgcgtcgc agcgggagtt gtcgcagccg cgctgctcgc
     1081 cgtgctcggg atcgcggtcc agcgatcgat tgcctaccac cctgttttcg tcgaccgaat
     1141 gcaagcgcaa gaacgtgccc tccagaaagg ccccgctgat ggcgaagccc aaagataagc
     1201 agaacgagtc atcgatgttg ttcatgccga ccgtcaccaa ggcgacgttg gcgatcaaca
     1261 aggaagctgc cggtgccggc gtactcggcc tcgtcggatt cgcggcgcta ttcgtcgtcg
     1321 cgatcgtgtt cttcgtgacc gagaactact tcgtgtcgat ggtgatcatg tcgttcgcgc
     1381 tgctcgtcta cgtgacgggc gccgagctcg cgaagctgtt cttgacctcg ttcacgatct
     1441 tcttctcgag caagcacgtg atacccgcgg ccgcctatct acaagacacg ctggtgccgc
     1501 tgcgcaacat cctcatgatc cggcgcgacg gtgagggcaa cgtaaaggcc ggccctctcg
     1561 tcgagggaat gacggtgaag ctgccggaca atccgctcgt gcgcgacatc cagacgttgc
     1621 tcgagaagaa tcccgacctc gattacgccg agtacatcgc gcacgagtac tacgtgcagt
     1681 gccacgaggt gtacgaccac tcgagctcgc acctcgagtt cgtcgcgacc gcgatgccga
     1741 tgttcggcct gatcgcgacg atcatcggcc tcatcggcat gttcgaaggg ctcggcgccg
     1801 aggtcaccgt cgagttcttg tcgccgcagc tcgcgctcgc gctcaagtgc acgctgtatg
     1861 gcgtgttgct cgcggcgctg tacaagatca tcgcgtcgcg ctttgaccag cgcctgaagg
     1921 cactcgacta cgacttcgag acgttctgcc gtgcgctcaa ggtgctgatc gacaacaagg
     1981 ccgtgatcga ggtgcggtcg tgaagtacgt ccgcttcaag tcgaagccga agttcgacat
     2041 ctggccggtc gtctacatcg atctgatgac gcagatcatg atgttcttcg tgatcctctg
     2101 gtcgatcagc cagatgcaag cgaagaagaa aggcgtcacg acggcggtcg gcgatacgac
     2161 cgtgcacgaa gtgacgctgc ccggcgacgt gttgttcccg tcgggaaaga cgaagctcac
     2221 cccgcaaggc gaggaagtct tcgcgaagct attcaaagac gacaccggcg aggtactgac
     2281 gttcgagacc ggcgggctga ccaagcgcgt gctcgtgatc catggccaca ccgacagtga
     2341 tggcaagaag gacgagaact acgtgctcgg cttcatgcgc gcgatgaccg tctacaagga
     2401 gatccagaag tacggccccg agatcgtcga tcacgcggtc ctctgcacgc atgccgacaa
     2461 ctcgcctgcg aaggaggtcc cgttgttcac cggtacgctc acgccggcac agtcacaggc
     2521 gctcagcgag gccaagacga agaatcgccg catcacgatc gaggatcagg tcaacagcgt
     2581 ggtgaaggaa gaatgagagc actgggcgca cgctcggtca ccgccatcgt tctcctcgcg
     2641 ggaacggtca gcgcggcgtg gctgctcgaa cgctttcgag cggattactg ggcgcagctc
     2701 agcccgtggg tcacgttcct cgcgggtgca ttcgcattcg cagccggcac gctcgtgttc
     2761 gtgtactgga gtctcggcga gcgcagccga tcctacctcc gcttcggcga gaagctgccg
     2821 ttcggcgagg agacgtcgct gctcggcgta cggacgcgca cgctgcttct cggtcacgca
     2881 ccctacgtgg aggtcgtcga cgtgccggcg cgagccatgc acgcgctgta catcgcgatc
     2941 ttctttggcg tcgcgctgat cagcctcgac aaccgcgcag tctcactggt ccgcgacgtg
     3001 cccaaccgct tcgatcgctc gagcatcgac tactgcaagc cgccggcgcc gccgaagcaa
     3061 gtgaagaagc aagaccaggg ctgcaagctc gtcgagcgcg cgtacaagct cggctacgcg
     3121 aagagcctcg gctcgtgcgc gcccgggcac gacgaggagc gccaggtcca gcgcctgtgc
     3181 acgcgacgcc agctcgacga gccgctgctg cactatgcgt ggcgccggct cgacaaccag
     3241 atcgacgatc tgcgcacgat cgacgagggc cccggcttct gggagctgtt cacgcgccag
     3301 ctcgatcatc tcgagacgat tgtcgagacc accgcgaaca cggtggcgat gcgaccacgg
     3361 tcgtcgcatc acgtgttcac gaacctgccg gatccgcgtc cgcgactgcg cgatcgcgtc
     3421 ggcaggatgc tcgagcgcgg atgcggcgcg cggctcgcgc atctccagca tttcccgccg
     3481 atgccggccg ggccgcaggg cgccagcttg atgttcgagc aagtgctcga ccagctgatg
     3541 ttcaatccga tctacaagcc gatcgtcgcg cagtgtgagg agatcgtcgt gcactggagc
     3601 gcgcccgcga atgcgtgcga gcagatccaa gccaatgcgg agaaggcgct cgacaacctc
     3661 ggcgcgctcg gctcggtgcg cgatgtcctc gcgtggcgcg acggacgagc cgagctccac
     3721 ggcttcgatg cgaagcacgc gctcgatgtc gcgacgccgt cgcgcatcgt cagctttcag
     3781 tgcctgatgt tcgacgacgc cgccgagccc gcgccggtgg ccgagcactc gtttgcgctc
     3841 gatggcgtta cgctcgcggt gcgcgagacg cgaacgaagc cgctcaccgc cgacggcgcg
     3901 agccagatcc ggctctacaa gcagctcgcg gcgctgctca cgccaggctt cggctacggc
     3961 cgcctgacca gtaaccaggc gatcggcgct gtgcccgagg aagcggcgat ggccgagtcg
     4021 ttccgccagc cgacgttcat gctcacgcgg ctcgatctcc tccgcgatgc cgatctgttc
     4081 ctcggcaacg agtggctggc gaagcgcccg gacctcctcg ccgtctatcc gtatcacctc
     4141 catctcaaga acttcatcga gatcttccgc cgccagtaca aggaacatcg aggtcggcta
     4201 tgaagcgcgc actcgtcaag ctcgcgctgt gcagcgtcgc gttgtgcggc gttgcgcccg
     4261 tcgggtacgc cgaaccggcg caggaccgca gcggctacat cgccgagctg atcgacgcga
     4321 tccgcgccac cgaccacgcg gcgctcgcga acacgcgcaa gtacatccag atcgtcgagc
     4381 gcaacaagtg ccaagcgccc gagatgacat tgcgcgtggg ttgcatgctc gaagcggccg
     4441 ctcagaattg caagcagctg cccgccggca agcgcacccg tgtcaccgat ccgagcggtc
     4501 cggcctctgg gccggagcgc gaggcgtgcc agcgcgcgtc cgatgtgatc gcggcaaacc
     4561 agctgagcga gcggctgttc gtgccgaacg acgtgcgcta cgcgatcatg agcaagcagc
     4621 gcgatgcgcg caccgcgatc gcgcgcgagc tgcaccaccg ctacgcgcag ctcgtcaccg
     4681 agctggcgat gtcgaagtac ttcccggggc cgcgcgccga cagcaaggcg ctgggaacgg
     4741 ggatcgatgg cttctgcacc gacgtctccg gtacgcgcga cctatcgtgg caatactgcg
     4801 ttgccgcgat cgtgtggttc gtggcgacgg acggcgccga gccggaggga acgtctcgat
     4861 gaccaaagcg cacatggcga tgctcgcagc cgagaacaag aagctcgagc aagccggact
     4921 ctatcgtccc gagacgattc atcccggcca cgtcgtcgac ttcacgacgc aggattacct
     4981 tgggctcgcg cgacacgaac gcgtcatcgc cgcagcacgc gcggcgctcg acaagtacgg
     5041 cctcgggctc ggctcggcgc gcccactcac cggcacgcgc gagatccacc gcgagctcga
     5101 acaatcggtc gcgcgcttcc tccaggtccc cgacgcgatc gtgctcggct cgggctacct
     5161 cgcgaacctc gggttctacg aggcgatgtt cgacagccgt gactgcctgt tctgcgacgc
     5221 gctcgtccat ccgagcaccg ccgagggcgt acggctgtcg tcggcgaccg cgttcccatt
     5281 ccgcaacaac gacgtcgatg atctcgagga caagttgcga cgttctcgct cggcacgctt
     5341 ccgcgcgatc gtcaccgacg gcgtgttccc gttcgacggg cgcgtcgccg agctcgatgg
     5401 catctgcgca ctcgccgaaa aatacgatgc gatggtggtc ctcgacgatt cactcggggt
     5461 cggcgtgctc ggtgagaccg gcaggggcac gcgcgagcta cgcggcgtga tgcagcgcat
     5521 cgacgtcatc acgggcacgt tcggcaaggc gctcggcggc gcgggcggcg gctacatcgc
     5581 gggcaagcgc gaggtgatcg gctggctgcg ccagaaggcg atgccctacc tgttttcgac
     5641 ggcactgtcg ccgccgctcg ccgccgcggc tcacgccgcc atcgaactgc tcgagcgcgg
     5701 cgaagcaccg ctccccacgc tgcgcatgcg cgttgcgatg atgaagaagg agctcgagat
     5761 ccgcggctac aagatcctcg gcgagcatcc gtcgctcgcc atccaggtcg gtggtgtcgt
     5821 cgtgcttcag aagctcgtca accagctcta cgagcgcggc atccacgtca acggtctctg
     5881 ctacccggtc gtgccggaag gccaggcccg gatccggctc gagctgagcg ctcggcacag
     5941 cgacgaggac atgacgcgcg cgatcaatgc cttcgatctc gcggggcggg cgctcggagt
     6001 catctgatga tcgagctgcg ctgcacgctc gaccgaacgc gcattgcacc cgatgtcgcg
     6061 acggaagtcg gctgccagct cgagctggtg ccgtcgcagg gtgcggccgc cgccggctcc
     6121 gtcgcgctca cgacgaatct gtgcctcgtg ctcgattgca gcgcgtcgat gctgggcgcg
     6181 aaggtggagg ccgcgatctc ggccgcgaag ctgatcgtga gctcgatcga cgcccgccac
     6241 cggatctcgg tcgtcggatt cgcgacatcc cattgggtcg tcgtcgacaa cgcctctccg
     6301 gcggaaaccg ggcgcgacgt gatcaacaag gagatcgagc gcctgcgaac gatgatcaag
     6361 ggcagcacga acctcggcga aggcattcgc cgcggcatca agacggtgca acggcagcag
     6421 gccgacgcca gcgtgctcgt cgtcctcacc gacggtgtcg cggactcgca ggccgacgcg
     6481 gagctcgcca gcgccgaggc aaacgaggcc ggcgtgcaag tctttgcggt cggcattggc
     6541 gaaagcttcc gcgccgatga tctgctgaag gtcgtcgcgc catccggtgg cacgctgctc
     6601 ggcgagcgcg cggcggatcg cctcgagacc gccttcgccg agctcttgaa acgcatcgag
     6661 agcttcgtcg cgaccggcgt ccagctcacg atctcgccgg cggcgggcgt cgtgctcggt
     6721 gcgacgtaca agacgagccc ggagcgcatg ctcatcggcg acaacgtcga acgcgtgatc
     6781 gtcggcaatc tcgagcgcgg tatcgcgtac tcgttccacg tcgcgctgac gccgccgtgt
     6841 gcaggctccg gcgaggccga gatcgcgcgc gcgacgctgc gctacgacgt tccatcgcgc
     6901 gggcaccgcg gtgcgatgac cgaaatctcg atccggatcg catacgacgc acgcgccgag
     6961 gccgcgaacc ccgaggtcgt gcgcgcgcat gcgcgggccc agctcgccgc gctcgtcaaa
     7021 caactcgcgg attccgagac gcgcggagac cgcacgaagt cggccgagct gctcacgctg
     7081 ctcgccgagc gaagtgctgc gcatggcaac acgcggctcg tcgaggcgct gcgcgggctg
     7141 gcgtcgccaa ttccgcaggg cgagctcaac gcgctggtgc tcgcggcaac cgcgcgcgac
     7201 gtcgtcgagg aaccgcgtgc gcccgagcca gctgcagttc ccgagacgaa ccgaccacga
     7261 cgaacgctac cgacgccgac gcggccgtct gctgcggcgc gaccgccggc cgagaacgtt
     7321 cgcccgccgt caccgccaca accgacgccg gcaccgccgc cacaatcgac gccggcaccg
     7381 ccgccacaat cgacgccggc accgccgcca caatcgacgc cggcaccgcc gccacaatcg
     7441 acgccggcac cgccgccaca atcgacgccg gcaccgccgc cacaat
//
LOCUS       DASUFX010000041         5987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_840405,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000041 DASUFX010000000
VERSION     DASUFX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840405"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFV99_01525"
     CDS             complement(<1..724)
                     /locus_tag="VFV99_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5058002.1"
                     /translation="MTESKSIEKVLVANRGEIALRVMRTCRAMGIATVAVYSDADADA
                     PFVRFADEAVRIGAPPARESYLVIPSILDAARRTGASAIHPGYGFLAENAEFSEAVAD
                     AGLIFIGPPANVIRALGSKQAAKRVAQKAGVPTVPGYNGDDQTRLLDEAHGIGYPLLV
                     KASAGGGGKGMRVVHRREDLADAIERARGEAKSAFGDDTLLLEKYVERPRHIEIQILG
                     DQHGNVVHLYERECSVQRRHQKI"
     gene            complement(765..1466)
                     /locus_tag="VFV99_01530"
     CDS             complement(765..1466)
                     /locus_tag="VFV99_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058003.1"
                     /translation="MLKLILVPALLLAACGDNLHPDEDQVATGAAGSGSDAPGDTPDA
                     AVLTARFIPFADDCDNHGVRFEAQFGYSDGGAVANPICHYDFADGTSADTCVASHSLP
                     TAQIVRLTVTDPATGAMAVAEDSVIGPEIFDVIVDLSSGLSSSGRPFIAWSARTLYGG
                     AEAGKIQFVDFDPIQDVEILDPEQQIQPTGVVSVNRPGVYTVSVLGSVELDLGGCSKI
                     VSKTIDLTTCITGTH"
     gene            complement(1631..3241)
                     /locus_tag="VFV99_01535"
     CDS             complement(1631..3241)
                     /locus_tag="VFV99_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5058004.1"
                     /translation="MEQPYPVIASRIDLRSPEYRANHDANTAAVEKLKKALGEATIGG
                     GEKYTSRHKAAGKLLPRERIELLLDRDSYFLELSPLAGKDVPGHTTGAGFIGGIGKVS
                     NVEVVISANDSTVKGGAISEMTAVKSGRLNDVAEHNRMPTISLIESAGADLPNQSKIF
                     VPGGRGFRDLTRRSQHRIPSICLVFGSSTAGGAYIPGMSDYVVMVEKQAQVYLAGPPL
                     VKMATGEDTDHEELGGAEMHSRVSGVSDYLARDERDAIRIGREIVAHLNWRKGAAPLP
                     RVIEPPKYDAEELLGIASPDIKVPFDSREVIARIVDGSRFSEFKPLYGTTLICGWAHL
                     HGFPVGILANNGILFSDSAEKGAQFIQLCNQSDVPLLFLQNITGFMVGKKVEQEGIIK
                     HGAKLINAVSNSTVPAITIMTGASYGAGNYAMSGRAYAPRFLFTWPNHRIAVMGGKQL
                     AGVLDIIQREAAAKRGEAVDEQKLGVMKAMTESMIDKESDPYFATARLWDDGIIDPRD
                     TRTVIAIALSAAYSAPVRGTTSWGVFRH"
     gene            3273..3821
                     /locus_tag="VFV99_01540"
     CDS             3273..3821
                     /locus_tag="VFV99_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058005.1"
                     /translation="MANVIEEAKSGRASCRTCKKAIAKGELRFGEEAPNAFGDQPSMR
                     WHHMLCAAEKLPAELKAALDQFPGTVPNRAELDKAMADALSGGRAKPGGFPYADKAPT
                     SRAKCMQCSQTIDKDSVRIAVEREIDTGAMVTKGAGYMHPKCVAEYCETHDMDKDDLV
                     EGVRANSRLVPADLDKTIAEIG"
     gene            3887..4867
                     /locus_tag="VFV99_01545"
     CDS             3887..4867
                     /locus_tag="VFV99_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5058006.1"
                     /translation="MRLLPVLVLLSPALAHADRHVIEKGETVQHVADAYGCSLDAVLR
                     ANKLQTTLLEPGTVIEIPNCSLRTRAQTRTRTVTPRSRPTSDDDKARAALAVIDGATW
                     TAESKPKLVAAPAPKLVATPAPKLVATKPVGISDSLGVPWDGELLRGEQLPLGDGYRV
                     RRPELAFGVPYVVDHLRHAIAEVRALYPDVHTLAIGDISAEHGGKLAAHHSHQSGLDV
                     DVGFYFKQVPDGYPDHFAAADDNIDLQATWALLTAFARTTDLDDGVSMMFLDYAIQRR
                     LYQWAAKRGTPEADLEHLFQYPNGKDAQSGLIRHWPNHADHVHVRFKSRR"
     gene            complement(4851..>5987)
                     /locus_tag="VFV99_01550"
     CDS             complement(4851..>5987)
                     /locus_tag="VFV99_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058007.1"
                     /translation="ARRASGQVALGLKIAACGFAGALLVNFAWGFVQFKDNLWEHEWI
                     YKTADWAFFAVWVVVPIGLAVANWRERRELAIAIVVVSLLTWPPPVLAKAMYSWMPEG
                     KSSYVIDGALRMVRFGLFLVGFAKVPQVGAVIDRVAAASGLRLAATSLWVRVIAAISL
                     VLLTLMLVAGRGSQGGLELFKLATFTALIINIIAFMQFGVGALRTARGSVADLGRWPF
                     VISGAISLWAAGVTLGQMPWLYRMLYKGGSSSFGSDNAMQYAQALAVAMPILITGGVA
                     LLAVAIAGFGARSGNEDLRSQAQAKGAGFVTLTLVSLAIQQWMIPEAMKKATSAGGFM
                     MLSVLAAVASLFAIVMMAKLLRNGAEAIEAEPGLPPASVVSDGT"
BASE COUNT         1031 a         2052 c         1926 g          978 t
ORIGIN      
        1 tgatcttctg gtggcggcgc tgcaccgagc attcgcgctc gtagaggtgc acgacgtttc
       61 cgtgctgatc gccaaggatc tggatttcga tatggcgtgg gcgctcgacg tacttctcga
      121 gcagcagcgt gtcgtcgcca aacgccgact tcgcctcgcc gcgcgcgcgc tcgatcgcat
      181 ccgcgaggtc ctcgcggcgg tgcacgacgc gcatgccctt gccgccgccg ccggccgacg
      241 ccttgacgag cagcggatag ccgatgccgt gcgcctcgtc gagcagccgc gtctgatcgt
      301 cgccgttgta accgggcacc gtgggcacgc ccgccttctg cgcgacgcgc ttcgccgctt
      361 gcttgctgcc gagcgcacgg atcacgttcg ccggcggacc gatgaagatg agccccgcat
      421 cggcgactgc ctccgagaac tcggcgttct cggcgagaaa gccgtagccc ggatggatcg
      481 cgctcgcgcc ggtgcgccgt gcggcgtcga ggatcgacgg gattacgagg tacgactcgc
      541 gcgcgggtgg cgccccgatt cgcaccgcct cgtcggcgaa ccgcacgaat ggcgcgtcgg
      601 cgtccgcgtc gctgtacacg gcgaccgtcg cgatgcccat cgcgcggcag gtccgcatca
      661 cgcggagcgc gatttcaccg cggttggcga cgaggacctt ctcgatcgac ttcgactcgg
      721 tcacgccggg acgctaacac gcacgccgac gtgaaagtga gtcgctagtg cgtgccggtg
      781 atgcaggtcg tcaggtcgat tgtcttcgag acgatcttgc tacagccgcc gaggtcgagc
      841 tcgacggaac cgagcacgga cacggtgtag acacccggcc ggttcacgct gaccacgccg
      901 gtcggctgaa tctgctgctc ggggtcgagg atctcgacgt cctggatcgg atcgaagtcg
      961 acgaactgga tcttgccggc ctcggcgccg ccgtagagcg tgcgcgcgct ccacgcgatg
     1021 aacgggcgac cactactcga caaaccactc gacagatcga cgatgacgtc gaagatctcg
     1081 gggccgatga cgctgtcctc cgcgaccgcc atcgcgccgg tcgccggatc ggtcaccgtg
     1141 agcctaacga tctgcgcggt cggcagcgag tgagacgcga cgcatgtatc ggcgctcgtg
     1201 ccatccgcga agtcatagtg gcagatcgga ttggcgacag cgccaccgtc gctgtagccg
     1261 aactgggcct cgaagcgcac gccatggtta tcgcagtcgt ccgcaaacgg gatgaatcgc
     1321 gcagtgagca ccgccgcatc gggcgtatcg cccggtgcat cggagccaga accagccgcg
     1381 ccggtcgcga cttgatcttc atcggggtgc agattgtcgc cgcacgctgc gagcagcagc
     1441 gcgggcacga ggatcagctt caacatgtgc gggcctcctg gtaggacacc cgcggcctct
     1501 gcaacgaaag ttcctgatcc ggaaccttct ccgttcgcgt gcttgcgatt tcgtgagctt
     1561 gtttaacgcg cgccgacgag gcaccgcatt gtgcccgcat gcagaggcgt cactcggtga
     1621 ttctccgaga ctagtgccgg aacacgcccc acgaggtcgt gccgcggacg ggtgccgagt
     1681 atgccgccga caatgcgatc gcgatgaccg tgcgcgtgtc gcgcggatcg atgatgccgt
     1741 cgtcccatag gcgcgcggtc gcgaagtacg ggtcgctctc cttgtcgatc atgctctcgg
     1801 tcatcgcctt catcacgccg agcttctgct cgtcgacggc ttcgccgcgc ttggccgctg
     1861 cctcgcgctg aatgatgtcg agcacgcccg cgagctgctt gccgcccatg accgcgatgc
     1921 ggtgattcgg ccacgtgaac aggaagcgcg gcgcgtaggc gcggccgctc atcgcgtagt
     1981 tgccggcgcc gtagctcgca ccggtcatga tcgtgatcgc tggcacggtc gagttcgaca
     2041 ccgcgttgat gagcttcgcg ccgtgcttga tgatgccttc ctgctcgacc ttcttgccga
     2101 ccatgaagcc ggtgatgttc tgcaggaaca acagcggtac gtcggactga ttgcacagct
     2161 ggatgaactg cgcgcccttc tccgccgagt cggagaacaa gatgccgttg ttcgcgagga
     2221 taccgacggg aaagccgtgc aggtgcgccc agccgcagat cagcgtcgtg ccgtagagcg
     2281 gcttgaactc ggagaaccgc gaaccgtcga cgatgcgcgc gatcacctcg cgtgaatcga
     2341 acggcacctt gatgtcgggc gacgcgattc cgagcagctc ctcggcgtcg tacttcgggg
     2401 gctcgatcac gcgcggcagt ggggctgcgc cctttcgcca gttgaggtgc gcgacgatct
     2461 cgcggccgat ccgaatcgcg tcgcgctcgt cgcgcgcgag gtagtccgac acgccggaca
     2521 cgcgcgagtg catctcggcg ccgccgagct cctcgtgatc ggtgtcttcg ccggtcgcca
     2581 tcttcacgag cggcggaccc gcgagataga cctgcgcctg cttctcgacc atgacgacgt
     2641 agtcgctcat gcccgggatg tacgcgccac ccgcggtcga gctaccgaac acgaggcaga
     2701 tcgatggaat ccggtgctgc gaccggcgcg tcagatcgcg aaagccgcga ccgcccggca
     2761 cgaagatctt cgactgattc ggcaggtcgg cgccggctga ttcgatcagc gagatcgtcg
     2821 gcatccgatt gtgctcggcc acgtcgttga gccggccgct cttcacggcc gtcatctcgc
     2881 tgatcgcgcc gcctttgacg gtcgagtcgt tcgccgagat cacgacctcg acgttcgaca
     2941 ccttgccgat accgccgatg aaacccgcgc cggtcgtgtg cccgggaacg tctttgccgg
     3001 cgagcgggga tagctcgagg aagtacgaat cgcgatcgag gagcagctcg atgcgctccc
     3061 gcggcaacag cttgcccgcc gctttgtgac ggctcgtata tttctcgccg ccgccgatcg
     3121 ttgcttcgcc gagcgctttc ttcagcttct cgaccgcggc cgtattcgca tcgtgattgg
     3181 cgcggtactc gggcgaccgc aggtcgatcc gtgacgcgat gaccgggtaa ggctgttcca
     3241 cgcgaaggaa tctagcagta ggtttgcagc aaatggcgaa cgtgatcgaa gaagcaaagt
     3301 caggccgagc aagctgccgc acctgcaaga aagcgatcgc gaagggcgag ctgcggtttg
     3361 gcgaggaagc gcccaacgcg ttcggcgacc agccgagcat gcgctggcac cacatgttgt
     3421 gcgcggccga gaagctgccg gccgagctca aggcggcgct cgatcagttc cccggcacgg
     3481 tgccgaaccg cgccgagctc gacaaggcga tggccgacgc gctctccggc gggcgtgcca
     3541 aaccgggcgg attcccgtac gccgacaaag cgccgacgag ccgcgccaag tgcatgcagt
     3601 gcagccagac catcgacaag gacagcgtcc gcattgctgt cgagcgcgag atcgataccg
     3661 gtgcgatggt caccaagggt gccggctaca tgcacccgaa gtgcgtcgcc gagtactgcg
     3721 agacgcatga catggacaag gacgacctcg tcgagggcgt gcgcgcgaac tcccgcctcg
     3781 tccctgccga cctcgacaag acgatcgccg agatcggcta agtcgcttca cttcgataag
     3841 ccgtagaaat tggcgcaccc gccagcggcc gggcgaccat ttcggcgtgc ggctcctgcc
     3901 ggttcttgtg ctgctttcgc ctgccctcgc gcatgccgat cgccacgtca tcgagaaagg
     3961 cgagacggtc cagcacgtcg ctgacgcgta cggctgcagc ctcgacgccg tgctgcgcgc
     4021 gaacaagctg caaacgacgc tgctcgagcc gggcaccgtg atcgagattc cgaattgctc
     4081 gctacgtacg cgcgcgcaga cccgcacgcg cacggtcacg ccgcggtcgc ggccgacgtc
     4141 cgacgacgac aaggcgcgcg ccgcactcgc ggtgatcgac ggcgcgacat ggaccgcgga
     4201 gagcaagccg aagctcgtcg cagcgcccgc gccgaagctc gtcgcgacgc cggcaccgaa
     4261 gctcgtcgcg acgaagcccg tcggcatcag cgactcgctt ggcgttccgt gggacggcga
     4321 gctgctacgc ggcgagcagc tgccgctcgg cgacggctat cgcgtgcggc gtcccgagct
     4381 cgcgttcggc gttccgtacg tcgtcgacca ccttcgccac gcgatcgccg aagtgcgcgc
     4441 gctctatccc gacgtccaca cgctcgcgat cggcgacatc tcggccgagc acggcggcaa
     4501 gctcgccgcg catcactcgc accagagcgg gctcgacgtc gacgtcggtt tttacttcaa
     4561 gcaggttccc gacggctatc ccgatcactt tgccgcggcc gacgacaaca tcgatctcca
     4621 ggcgacgtgg gcgctcctca ccgcgttcgc gcggacgacg gatctcgacg acggcgtctc
     4681 gatgatgttc ctcgactacg cgatccagcg tcgcctctat caatgggccg cgaagcgggg
     4741 cacacccgag gccgacctcg agcatctgtt ccagtacccg aacggcaagg acgcgcaaag
     4801 cgggttgatc cggcactggc cgaatcacgc cgatcacgtg cacgtgcgct tcaagtcccg
     4861 tcgctgacga ccgatgccgg cggcaacccc ggctccgcct cgatcgcttc agcaccgttc
     4921 ctgagcagct tcgccatcat cacgatcgcg aacaggctcg cgaccgctgc gaggaccgag
     4981 agcatcatga atccgcccgc ggacgtcgcc ttcttcatcg cctcggggat catccattgc
     5041 tggatcgcga gcgagaccag cgtcagcgtc acgaaccccg cgcccttggc ctgcgcttgg
     5101 ctgcgcagat cctcgtttcc cgaccgcgcg ccaaatccgg cgatcgcgac cgcgagcaac
     5161 gcgacgccgc cggtgatcag aatcggcatc gcgaccgcga gggcctgtgc gtactgcatc
     5221 gcgttgtccg aaccgaacga cgacgaaccg cctttgtaga gcatccggta cagccacggc
     5281 atctggccga gcgtgacgcc ggcagcccac agcgagatcg cgccgctgat cacgaacggc
     5341 caccgaccga ggtctgcgac ggacccgcgc gcggtccgca gcgcgccgac gccgaactgc
     5401 atgaacgcga tgatgttgat gatcagcgcg gtgaacgtcg cgagcttgaa cagctcgagg
     5461 ccgccctggc tgcctcgccc tgcgacgagc atcagtgtca ggagcacgag gctgatcgct
     5521 gcgatcacgc gtacccacag tgatgtcgcg gcgagccgca aaccgctggc cgcggcgacg
     5581 cgatcgatga cggcacccac ctgcggcacc ttcgcaaatc cgacgaggaa cagcccgaac
     5641 cgcaccatcc gcaacgcgcc gtcgatcacg tagctgctct tgccctccgg catccagctg
     5701 tacatcgcct tcgcgagcac cggcggcggc caggtgagca ggctgacgac gacgatggcg
     5761 atcgcgagct cgcgacgctc gcgccagttc gcgaccgcga ggccgatcgg cacgacgacc
     5821 cacaccgcga agaacgccca gtccgcggtc ttgtagatcc actcgtgctc ccacaggttg
     5881 tccttgaact ggacaaagcc ccacgcgaag ttgacgagca gcgcaccggc aaagccgcac
     5941 gcggcgatct tgaggccgag cgccacctgc ccgctcgctc gccgcgc
//
LOCUS       DASUFX010000042        39519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_6587,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000042 DASUFX010000000
VERSION     DASUFX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 39519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39519
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6587"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3498)
                     /locus_tag="VFV99_01555"
     CDS             complement(<1..3498)
                     /locus_tag="VFV99_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5058008.1"
                     /translation="MRTAHDDVMDDLAALVAGEASAISKHASHLASCDQCRDAKHEAA
                     VLATRLAGAGSDYVPSNDLVDRLMTKLDEKPVEKKLEKTNENTIEKPVEKPIAAPTPI
                     AKRKLWIVAGAAAALAAGVVGVAMMKKGGDDASHTTVAGNGPIGKVKTISRAASEQGD
                     GIAIKDSATRSWRPLRAGEMLPTGAELRTDERTRVSLELADGSRMVLDHRTSVVFDAK
                     EPRRVKLVAGRIAADVAHVDNRQASITTPSGRVDVVGTRFSVTAADNLTAVQVVRGGI
                     VLTAANGAKDEVRAGEEGIIENGKLSVSAAPELVKDAQWSEIEQPKTKQTDQETTSGL
                     GALRAYKPGEKRDKDWNLALANHEVKVRISGPVARTEITETFRNDSDTQLEGVYQFPL
                     PADAQIDSLALDMKNEPDGFINGAFVDKERAAKIWRGVIDKATPKRIERDPLIQQEIV
                     WVPGPWKDPALLDWKRGGRFELKIFPIPAKGVRTIKLAYTQVVTPRGPQRQYVYPLPH
                     STDGSTVADKMKVDVEVRGAQQGSVRAAGYELKPDSARADVNAMTLEQGGFVPRGDLV
                     VDYRPDGDAEVRAWTFTGGAAAGPDDKLAAKKNVGIDQKVIDAQKVVAADARPTAVLA
                     LSPKLPRWRESKARDYMIIIDGSQSMVGERFTRASELAVSMVDDLDRRDRFTAMVCDS
                     ECRAMGDLRAPTTSAAGELKGWLGTQTPAGASDVVAALRAGHAGLKDLSRERWVLYIG
                     DGFASTGFRRVADVEKAIASTTGTGDVHVTTIGVGSDADSALLAAAARGGGGSYLAWV
                     PGQSVGTAAMAALESTYGTSLRDATVELPEGMTEVAAAGGTTAEKTTMTSLPTLRAGG
                     EVLIAARINGDVSGEVVLRGKVAGQKFEQRYPLKLAVSTANGNGFVPRLWASLAIDQL
                     ERQGKGEDRSRIVALSQGYGVMSRETSLLVLESQAMFDAFGVDRSQSAAKWTGEEDID
                     EVAATGTIPVPADNAPAPAPTMAKASMAPRAARDEEEMTMSLDAGGGGGTRWGGKGGV
                     IQAEKPADKAEKKAPAKDMPRAMPRPPVGGGRRIVIQMKKVWTRNAALGTFDGTSASI
                     TKAIADAQDALVKNPDSREKHRALVQALSYGGKLEEARDIANKWLERDKLDPQALGYI
                     ADILGREGKRELALRTLDGL"
     gene            complement(3498..4004)
                     /locus_tag="VFV99_01560"
     CDS             complement(3498..4004)
                     /locus_tag="VFV99_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5058009.1"
                     /translation="MIHDGRHRDALAACARTHHVVLGRLCMALLGSQADADEGVQETL
                     LRAHRAMASYRAEGSVKAWLCGIARHVCAHMLETRRRGKELLELVPTEGEARDSFALR
                     QRARVLRDALEKLKPSERESLALRYVADLSHREIAEACGLDEAAARKRISRALDRLRS
                     VMPTEEIE"
     gene            complement(4203..4469)
                     /locus_tag="VFV99_01565"
     CDS             complement(4203..4469)
                     /locus_tag="VFV99_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058010.1"
                     /translation="MTKLISFMIIGAFAIGCGHGSSKKQDTTTTNTGSGSGSGSDSGS
                     DATKTTTGDSQPCTQEVALECPDGQIDGCLKQPAEGDTHTCVAK"
     gene            complement(4517..6376)
                     /locus_tag="VFV99_01570"
     CDS             complement(4517..6376)
                     /locus_tag="VFV99_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058011.1"
                     /translation="MTRYELLDRIGVGGMAEIFRGKAVAAGGFEKPVAIKRILPHLSQ
                     DRRFVELLIAEAKVLSLLKHRNIVQIFDVGLGDDGQYFLVMEFVDGKDLGAVQRALEQ
                     QKRRRMPFDLVLHIGAEICEALEHAHTSRGPDGRPMALVHRDVSPSNVLLSRAGEVKL
                     TDFGIAKRAEQDATQGGAVRGKFAYISPEQARNEPLDPRSDVFSVGILMWELICNRRL
                     FSGLDDLAALRSVREATVPRPSEIDGRLPPEIDQILMTALAKDKTRRHATTGQLGQKL
                     RSLRYSLDQVVGDPASELAKLIDSTEELERQSQKVIAPRDQSGRFDFDGAEATVIRIR
                     TADAFSAREDQSISQARLVIDRFEEEETRLANLSGDQMRMLRTAAPIRDSGELTAEGA
                     PPARRARRDSDSDEEKTRAREPLDLGKLDDGYQPPFNTPGEDTRLIAPRDRRTPVPGM
                     RTASPSRSNRPSTVPPPMPLRGRGPTPGPPLPGLPMPPQMTGNSVPGMAPLPPTPQPQ
                     PLGSNLPMGMNNMPMGGAMPMQGAPPFAPSQPAGGYAGWAPPIAPQIPTRDATPTAQD
                     SFDQPLFPPRATSMPQVAGFGRTRKPQLKPWVLVVGAIVMALIAFAITRAFIG"
     gene            6411..7469
                     /locus_tag="VFV99_01575"
     CDS             6411..7469
                     /locus_tag="VFV99_01575"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5058012.1"
                     /translation="MDRIKRLLDEALANGLGSAAAVSVGDGGHEVFRYVAGVVRRVPD
                     RGQRVEESTRFDLASVTKPMATVAIAMVLVAERRLDLDAPVRTYLPGAATTATVRQLL
                     GHSAGCAAHVEFFRYLRATRPQNPREVLVERAMREPCGPPGIDAVYSDLGFIQLGAVL
                     ERAAEQPLEQAFTELVGEPLALAARYAPEALVNCVATELDDRGLVSGLVHDENCYFGG
                     RIAGHAGLFATIVDVAAFARAITDTAAGTPRGRFTTDVVTRFFTDTPTPGSTWRLGWD
                     TPSSTPGVSQAGDRWPRTGAVGHTGFTGTSLWLDLAKRRWVALLTNRVHPTRHGDTAD
                     RIKALRRAINDTVVETLG"
     gene            7489..7845
                     /locus_tag="VFV99_01580"
     CDS             7489..7845
                     /locus_tag="VFV99_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058013.1"
                     /translation="MRRRKALALVGLLGGVLIAALVGCAFVIWRAPDMAALIAFGATC
                     AAATTCMSLRHFGWLFLIVAVVYGVLAIGLYALHLLGLPFAAIVTGVAVLVVIILATM
                     MWALIPAGTEDDPYDD"
     gene            complement(7842..8792)
                     /locus_tag="VFV99_01585"
     CDS             complement(7842..8792)
                     /locus_tag="VFV99_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058014.1"
                     /translation="MAEGIAGPGFYPQPNEWTCGPFALKHALLALGRMVDVKQLASTA
                     KTHWWSGTDEIQLARAAREFECDLVLERRRDPEQARKLLVHYLREQTPVLLCVDEWTH
                     WITVLRAEDRRFVIVDSNDDPLLSLRTWPQLRTWWKYHDVDYSKTDPPVLYDLMAVTP
                     RFRTTVKADFSVERVKFLRRPENRRLALHWNEYLEDLLEICKPPSVRIVQPLSMGEFL
                     RRHAELLMTRVVYWHGDVNRDEVGRLLRDLRFVSETYGLVIPASMSRRALADLAILVS
                     LWVCADRGVDPMFGATGADVRAAGKSKSKRNGFRTNGRRH"
     gene            complement(8857..9264)
                     /locus_tag="VFV99_01590"
     CDS             complement(8857..9264)
                     /locus_tag="VFV99_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058015.1"
                     /translation="MVVFSNHRLGPSAELFVSDDVALTMLRETAAAARPFAEARWEVE
                     LVLWLDSRADAGTQLLDVSDIAWTPEHFERQRSFVIEAIQRAARCGEHALAFDRWRRM
                     VEQHPADSVQVGRRWQWPTNHAVNPDLADSNPS"
     gene            complement(9273..10043)
                     /locus_tag="VFV99_01595"
     CDS             complement(9273..10043)
                     /locus_tag="VFV99_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058016.1"
                     /translation="MSELITRLREILGGLAASDPRFRRFGAAQHHYHLAPPIAEGMLD
                     VELPADYLEYVTRLSAGGVGPYYGLIYVDRVAPIDAPTGVTAWQRALPISHLGCGYAA
                     LLTLDGPASGQVWLDARQLGILQPIRPSFTAFYLDWIDRIAHNQWLDGFVPPGQCAIT
                     AALTGYLGVCEQRLGIPPGSLDGDALRAALADLGPGAIEVTADGPLALFAPGDRVAPC
                     ATCARALQGLEDQGLRNDVVRAGVLPLPMRESAVTPTT"
     gene            complement(10047..10463)
                     /locus_tag="VFV99_01600"
     CDS             complement(10047..10463)
                     /locus_tag="VFV99_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5058017.1"
                     /translation="MFHRGELTVATKGRGTYDITGEVAKVVAASSAQNGLATVFIHHT
                     SASLIICENADPSVRRDLETFAAKLVPDGDRMFTHDAEGDDDMPAHVRSVLTQTSIGL
                     PIANGKLALGTWQGLYLWEHRTSPHRRHVSVTVIGE"
     gene            10522..12384
                     /gene="dauA"
                     /locus_tag="VFV99_01605"
     CDS             10522..12384
                     /gene="dauA"
                     /locus_tag="VFV99_01605"
                     /inference="COORDINATES: protein motif:HMM:NF008660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HEX5058018.1"
                     /translation="MIRRSLRYAEELTDAVKQQLALAYTAKDLLAFGLRSRMREGYSR
                     KDFRSDLMAALVVGVVALPLSMALAIACDVPPQHGLYTAIVAGVVCSLLGGTYFQVTG
                     PTAAFVVILLPIVHKFGVGGLLVAGMLAGLMLVGMGLARLGKLMQFVPHPVTSGFTMG
                     IAIVIGVLQLKDVFGVKLPRTEGVFEYFGALIDARHAFNFWDVGVAVITLVMLLMLPR
                     IIKRIPVPLIALVVAALLVVVVGHFAPESWGFHVTTIGSKFTYPLGGGEVGHGIPPLP
                     PLPVLPWQVADPGHPPFDLNLQVIRELLPSAFAIAMLGAIESLMAAVVADGMGGSRHD
                     PNAELIALGVGNIICPFFGGIAATGALARTATNIRAGARSPLAASMHAIFILACTIAL
                     APLVSYLPMAALAALLIIVARNMSEARHFFRLARIAPKHDVLVMLTCFTLTVVFDMVI
                     AVTVGVVLAALLFMRRMSVLTQVELETPTTLKIDVPPGIRVYEIAGPLFFGAAKSAME
                     ALHTVGDKDHTYVLDMQHVPAVDATGLVAIESMMDRLHRSKIKIIFAGLSTEMSDLFA
                     RAGIKRELGKVAYAPDVETAVSMAIVHAARTGRRADTSPSMRIPKVDDSGQPAA"
     gene            complement(12381..18500)
                     /locus_tag="VFV99_01610"
     CDS             complement(12381..18500)
                     /locus_tag="VFV99_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058019.1"
                     /translation="MELETLEALALADNRAAALSQLLPGSEDHDYYRCLHAQHRGAFD
                     EAQVILDAWHERHGDTERYRRMRLRQFWYRLGTSPDTAADDIRDLYGVSHWHEADAPD
                     VDPSRPTKLAAGAFTGDKLLTEAMSWTDLSQVTDEGLYELLDRTLDATQRRALLSRIG
                     HTPHERLVTLIHEDLEARGSGGFGSLNLHHQLTLDQLHALVKLDTKLLTNRAWVEAVV
                     RRMRPPAGVDIELDRDAREAYLRELWQFLRPLPATNNGLKEHVLWHLLDTLRQRNAPV
                     DSGLFAEYLQLPRSAAYVARDWLNGKRSDEIAARGVDWRNVTGLPPAGDDEALVRDYL
                     QRQVVTRGDAEQYAQWLDRTWLDGEIATAQLLNGAGDTDRATLVLGPTRAAALRDRVD
                     LVWCLHNPTRFAVDEPIILEADVKNVPELVVKVFRIDPLAYFQTNRKEISTDVDLDGL
                     AASHELVLRYSEPPIRRVRRRIELPMCTRGGSYVIDLIGNGMSSRAVIHKGRLRHVAR
                     IGAAGHAVTVVDEQGRLRADTRAWIGDREYIPDPNGTFVVPFSTSPTRTPMLLSCGDI
                     ATVQYLDLVREQYDLTLDLHVDRQALISGATAHAIARVQLTVSGQPVSLAALEQPRWE
                     ITLTDRHGVPTTKQQPLTLSDDDAAVLDIPVGEATDQIMVRVSGKLRVVSEQRDLDLS
                     AVQRASLASMHGGLATDALYLAHTASGWVLSALGKTGEPRAQRSLTVTLVHRWARTQL
                     NVELATDAHGRCELGELPGIERITATFGSVPQTWWLTSGGRPAQTIQVAPDTEIAIAL
                     PPDRDAHDVISRASIVEVRGGYPVRHPNVTLEPLTGGIVVRGLLVGEYAVRAPGLSEV
                     IIRVAAGTEISGHILAPGETVGVSRLAPVIAELTASDALHVRVVGASSRTRVHVIATR
                     FVSAPFAKPEYRLRTPLYRIDRARGALYVSGRELGDEYRYVLERRNARRFPSLLLDKP
                     SLLLNPWARRTTSTDVAVAAAGRAFAAAPGGAPGAGYGGPMQARGEPAVDESAYLGYD
                     FLAEPARVFANLTVDAHGALTLPLAELGNATSVSVIVDDPAGTSRRHLALAESPLATR
                     DLRLHIALAPESHVTQHKTIRPLLPGNAIVIEDLATAKLHLIDSVERAHAYLLALRDD
                     ATLREFSFVTRWHTLADTERRELYSKYACHELHLFLYCKDRAFFDAVVRPYLVHKRTK
                     TFLDHWLLDVDLASYLEPMRLARLNAVERALLAQRLLGDAALVRQLADEVGVQPPNPE
                     LDTRIIDALLGASTLEGGSELQTLQQHAVTTRQTMVMAEVATMAAPQAPPAPAPKRRA
                     EAKKMSKSIAAEEEYDALDMDLERRRAEAPMYRVVDKTQEWAENNWWHQTPQQSGADL
                     IEPNRLWRDLALHRDGVFLSQWLGLATNNFAEAMCALALVDLPFVAGSHQLAPDGPRL
                     TITAAANTLAGTSQLVPGELVETGAPLVVGQSYVRTDDRYRYVDGEQVDKYIEGSFIA
                     GVVYTCQVVLANPTSSRQRIAALIQIPRGSISLAGALATQTLDIALDPYGTHGHEYAF
                     YFPAPGRYGHFPVHVSRGGAIVAAAPGRVLEVTLGGDVLDPQSWAHISQRGSVADVVA
                     FLDQQSLADVDLERVAWRLRDRAAYDAILGALERRRVYAPVLWGYALLHRDPPRIHIW
                     ARALGAALLGAGPVLDMPVLGLDAEALDGYEHLELAPLINARAHRLGPKLRILNDGLA
                     AQYTRFLELVAHRPLPTSEDMLAAAHYLLAQDRADGALAALGRVDVTQLADRMQHDYL
                     AAYTSCLIGDITRARELTARWRELRVDRWSHKFSALANMLDELTGAAPSVVDALSRDQ
                     QQAELAAKQPTFDIALDREGVLIRNQHVTSLELRFFEMDVELLFSRQPFVQSDVSRFS
                     FIEPGHREYLTNPPAEHRVAWPPALRGKNVVVEAVGVGQRKARIHYANDLATYVAHQY
                     GQVRVQRASDHAALAATYVKVYAQKHGGAVAFYKDGYTDLRGWFDYASLSTNDLDHVE
                     KFAILVSSDQSGSAILEAGPPAR"
     gene            18531..19274
                     /locus_tag="VFV99_01615"
     CDS             18531..19274
                     /locus_tag="VFV99_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5058020.1"
                     /translation="MSRVLLILLLVAGVASVDAAPSKSDAAETKTKKKKRKKKKEKDD
                     RTPNKRTKANMPRGFVWPPSKTMEEAADACELQLDELGVAYETAAPAGKIVRPLKISG
                     DIAGITYTSLNGRNQTFDCQLVLTLANFAPKLQELGIKEVKFGSAFRYTKVRVGGKTK
                     NVLSRHALGIALDVYAFVDSEGHEANVHHDYKRGDELLHELERTANNSGMFRILLTPR
                     NDPKSHYDHFHFEANPDYANELTAEKPAS"
     gene            19271..20167
                     /locus_tag="VFV99_01620"
     CDS             19271..20167
                     /locus_tag="VFV99_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5058021.1"
                     /translation="MKFAALACALFACGGAGDDDMMGGPDGGVTGGDGSMTTDDSGML
                     CMKPALDQPWLANLVSGAVTGLAGAPRFTNTQRQTARTFLQNQLTQIGWTPQLHSYNT
                     GANVYATIPSTTGETKQIVVGAHFDTVSGSPGANDNATGSAAVLAVARYLKDMNCRKF
                     AVTVVFFDEEESGLFGSRAFAHTLQVANVHAVHTVDQVGWDQDNDLRFELELPTTTLE
                     TEYRAAAQTLGVPVTKTSTQGTDHQSFREKGFPAMGLTEEYVGNDTSPYRHMAQDTAA
                     TVEQSYLQLGSKLVALAVMNEL"
     gene            complement(20171..21457)
                     /locus_tag="VFV99_01625"
     CDS             complement(20171..21457)
                     /locus_tag="VFV99_01625"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5058022.1"
                     /translation="MLSLRSHVETLVRTAPPEQVADRGPEVDPTSVGLDRKTIDAIWR
                     ATIGAYRTGLYPALALCIRRRGQVVFDRSIGHITGNAPVDPPDAHKVIASPKSLFNMF
                     SASKMVTAMLVHLCDQRGLLHLDDPIAMYLPGFGEHGKHRITIRHVLTHRAGIPNIPP
                     QFADVSLLERPNEIISLLARQKPRWRAGQRLAYHALTGGFVLGAVIETVTGKPLRQVM
                     HDELLAPLKFDAFNYGVPPARLREVASNAFTGPPVLPPLSLMLKRLFSVSFNEAVRIS
                     NDPRFLTSVVPAGNIVATANEASRFMQLLLNGGELDGVRVFETRTIQRAIAEQTFFEL
                     DLTIGAPIRYSMGFMLGARVASLYGLNTPRAFGHVGFTNVFVYADPSRDIAVALMTSG
                     KPALSPGVTRTLWIMQTIASKMPRDGAGPLRSKPGR"
     gene            21543..21986
                     /locus_tag="VFV99_01630"
     CDS             21543..21986
                     /locus_tag="VFV99_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5058023.1"
                     /translation="MAVRQNLDELRSAVRAKGLRATPSRLAVLELLRASDTPMSHGDV
                     ADRLATEAWDRATIYRNLTDLAESGLVRRTDVGDHVWRFEAVSDDHEASAHPHFVCTE
                     CGTIACLPEIELAVRASRASRGKAPRAVKQRQVEVHVRGLCDSCQ"
     gene            complement(21987..22991)
                     /locus_tag="VFV99_01635"
     CDS             complement(21987..22991)
                     /locus_tag="VFV99_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058024.1"
                     /translation="MVKHAVIALALVGCGSFEDPDIVIDFRVLAMSASVPEQVVDIDI
                     TNPAPPPNLLDQLVPTDVCALLSDRNFERRIRWSMTLCNLNSDERCPDGGPKTVIGAG
                     LWDDPEGDDKDMAGAPPTFCATVPVDGNLLGVILDSLDSDQFKGLGGVYYGVSLVVGG
                     EDGDPDLDLYAAKSLRVQPRIPPELQANNNPSLAGVDVRLAEDQEFQPLTLKRCEDLD
                     NAGIEPPVVAPRQKIRFMPIEPDGVREEYVVPTIDGMSRMFTESPTYQFLASAGGFSA
                     GSTGGPRDPFGNPAPLFTDWTAPAAKDLKGPTDVSFWFVQRDERLGLAWYEACVRVVP
                     "
     gene            complement(22985..25633)
                     /locus_tag="VFV99_01640"
     CDS             complement(22985..25633)
                     /locus_tag="VFV99_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5058025.1"
                     /translation="MFRVVVALVALCAIAHAQGSGSGSAAPPAGKITKSPKLIQAVAP
                     EYPPAALAAGKTAKVKVRIHIDATGVVTKVDVLEPVGDGFDEAAVAAAMQYVFEPAEI
                     DGKPAEVAVETAIGFTIDLKVEEEPPPPPPKPDQQVGPPNHAGIMSDPITLQGVALER
                     GTRKKLAGVIVSIVELGLDAVTGDDGTFYFHGVAPGHYQVLAVDPKYDRFERPVVIEK
                     REALEVRLWMRPRGGNPYETIVEGEREQLEVTKRTLHRQQFTSVPGTFGDPVRVIQTL
                     PGLQRPPFGLGLLLVRGSNPDDTGIFLDGHEVPSLFHFLGGPSIFNADMLESIDLYPG
                     GFPGRFGRHHGGVVALESRPTKSDGIHGAAKVDLIDSGGYIRAPITKDVSAAVAGRRS
                     YIDLILPLFLPEPTAGSQRIVTPVYYDYQGRVDWDLHREGRLSLFAIGSSDTLHVFQK
                     DPDANTSANLNTAVSFFRVIGTYTRPLGGDLKLTLSPAWGADSITFAGAQAETSGPFT
                     SLSVKNKALSYRMRVNGKLRPTLTLDTGLDLMSRVTTFEALVPVDDTLIETQGVDIPP
                     SKLFRGSQSLGLGGYVDLGIDATANLKLIPSLRLDGYMLDGNTRQSVDPRLVAKYKLS
                     SKFVGKAYVGRFSQPPQPEALDTRFGNPDIQLEHGYHYGLGYEWKPERLWSVDSEIYY
                     VDRRDVIVFTPDVIMNSDGTFSYVNFRNTGRRASYGLEAIIKREISEKAFGWLSYTYS
                     RSRQTNREGEGWFATSFDQPHVMNAVASYKPGAGFELGARFQLASGRPDTKVIGATFD
                     ADCGCYDPVTTGMRTHRVDTFMQLDVRIEKTWLFQLWSIGAYIDVINVTNRKNQEGID
                     YDYRFRESTPVTSFPIFPTIGVRGTW"
     gene            complement(25650..26522)
                     /locus_tag="VFV99_01645"
     CDS             complement(25650..26522)
                     /locus_tag="VFV99_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5058026.1"
                     /translation="MIAVAVVMNRTPAFAGDRITELTQMLSSSSEKTRMSAVLSLARL
                     GDKRTMKPLVTALQDPNAQVRAIAATALGKLGHKAALPALKNAATDDLDDTVRAKAHE
                     AAVLVAKANNIKDDQLTPPAAGSAQARKKTTGFGRSPHAVADQPDLYVTIKGTNDDSP
                     GKTDKNTRKLHADILKLALQDQLKAAPQVTMVAADAQRWGLDPRMIDVSVVKLDVVQD
                     GTYMVVDAELRLAITDDKGKMLSFLSGGAKVQVPVAKYKPKFLPSMRKDALEGAMKGM
                     FDKLLAHLRQMTTT"
     gene            26658..27494
                     /locus_tag="VFV99_01650"
     CDS             26658..27494
                     /locus_tag="VFV99_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058027.1"
                     /translation="MSEQPYRIGVLAGALVLVAVITYLRFCGSVGLPPKPPPPTGPSG
                     TQSELLSKSTTSPTIYKGFLDSDAAAAGVRAPTIDEMSRKLSYRVDEARHVLEPGQPP
                     LEVAGLRIRLERTSDQVVMVIQNMLGSDAAYEVTSSPSTGSALCKGARALPFNAMVIS
                     KGQSERRTECTWRDGMSIIVTKVETIELAPLPAWYISQVPPQVVGIEDRLARGHRGVD
                     TKEKCSAVQSQVVRTGLDRGDIGWRDLIDFYSRHRCQTYQFPSTYRAFKSDGERRVPA
                     GG"
     gene            27580..27942
                     /locus_tag="VFV99_01655"
     CDS             27580..27942
                     /locus_tag="VFV99_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5058028.1"
                     /translation="MTKSELIEKISETLKLPAGKAEAIVNTIFDSMVKALERGEGIEI
                     RGFGSFTVRKYKAYEGRNPRTGETVHVAEKRLPFFKVGKDLRERVNAGLGTPLPADKP
                     DEPDDTDPNEPEYDDDED"
     gene            complement(28255..30330)
                     /locus_tag="VFV99_01660"
     CDS             complement(28255..30330)
                     /locus_tag="VFV99_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5058029.1"
                     /translation="MWVHDLRDADASCGGKAVGLGRLLAAGLPVPPGFVIDDRAFRSV
                     VGELAITDTAHIGHVLEEAAQRIAIARLPPELLDAVGSRAHELGHVVMVRSSATIEDG
                     AAGSAAGVFSSRTAVPVDEVWDAIRAVWQSALTPLAAAYALRRGGTIAIGVIVQEYVS
                     GTPLVVYTRPPGQPASREILIQHHDHVTRHSRDDLPREIEHQHAAVLALRAETALDQP
                     HGVDVELVQLRKQSGFDVAIETMIVQARPIVHPTTRTLAAPPPAVIAPLADGRTWTWD
                     VAHNPDPLSVAQQGLVERVERAGIAPWSLRICAGFLYSAPRGEELPIVRDAAELIAHA
                     TELQARLERTLVDGGKPLPLAEAVERYLAFYAIWARELSPLISAARAMLPAKLRAHGS
                     AHPDAIAASLVERPASTVEATLRAAARGDLDDDVVEARLGVLAPAWDVVAPTYAERPG
                     LLRDAIARAKLTQQERARDDRKTGEQPAVPNPALFAEHAVEVDIARVAADLAERDDML
                     FARAQWIVRRALLARSSELKLRGDDIFWLPLDETIAATAFDPDDAHRRAAGARAAAER
                     AAQWEMPIVVGGDAAKPGPSLRGVGTGARVAGRVVRFASLASAIAVGSGDVVVTRAVT
                     PALAVLVAGCAALVSETGGLLDHGAALARELGIPCVVGCRDAWTKLSDGMIVTVDGDG
                     GTVTPSSDR"
     gene            30354..31139
                     /locus_tag="VFV99_01665"
     CDS             30354..31139
                     /locus_tag="VFV99_01665"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX5058030.1"
                     /translation="MRLAVIVSLFALTAATARADDSKLAKEDVPLQAKATLRGKNKLC
                     CGYPLVDDMQWALRFYWMVLESDYDEPASPNVPRSGRGKEQVSETARVDVYTPLGFFL
                     GRMSEKFAWALRLEGSGLMADGRVINYAGKCDVGYATKVAGQPIKATCFEQLDVEQFP
                     FGRGAGFRPLIPFKSVAIDPRLVPLGEPLYIPEFDGMMLPDGSIHDGCVRADDTGGGI
                     KKRKMDFFVVTYGNFRFLLDQLQGVTWITPHVEAPRCEYLRER"
     gene            complement(31136..31576)
                     /locus_tag="VFV99_01670"
     CDS             complement(31136..31576)
                     /locus_tag="VFV99_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058031.1"
                     /translation="MLDGLFVTPAVTAIGEALATAHADGKLAVIGNAKLVTALRDAKR
                     NVVPIGLSERAAKKLTNALADLSSIEDRSLAAVIGVDIATDDGWEITLKDWTRVVRDG
                     GVLVFVDRGHAPEASRRALCAGLSEIEQRHAGRVVVTSGIVSHL"
     gene            complement(31680..34229)
                     /locus_tag="VFV99_01675"
     CDS             complement(31680..34229)
                     /locus_tag="VFV99_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5058032.1"
                     /translation="MSEPAVSPIHDRSRVAIANRADGGIAYWIGKLYGFAALVGVTAT
                     ALFGFILYSYFSVRTDDVGPGTRHDLAAYSKTVPAVSRMYAADGTLLGEFAQEWREVV
                     PYEAIPKQLVDAFLAVEDHDYFNHRGLYFKGIARAVWTNITAGDFAQGGSTITQQVAK
                     QFLGSEKSLSRKAKEAILARRLEATYSKRAILAVYLNHIYLGAGAWGVAAAARRYFQK
                     DLDQLTLDECAMIAGLAKAPTAYSPVHRPKVATDRRNIVLEKMALYGFASRADVDKAK
                     ATPIKLDMYRDVFPDRMPYYAEQIRRYMTERYALQSEGLRVETAAEPTWEAAAYANAD
                     YGAHNQDKRQGWRGPEWRIDGAARATFLERQKQLYGNGPLVRGKRYLALVDKVGDDKA
                     ELLIGDKRVTLPMRGMSWAAKWEAGNAENDKTIESPREALKSGYVVWVSRETKTIEKF
                     REYGLPDKINPSWVYSDNQKDWDEAHTDVVRLEQVPHPQTTIFTGDHRTGYVVAMVGG
                     YDYDRSVFNRAVQACRQPGSTYKPIYYALGLDEGYGFDTVLNDIPVQIVDPDTGAIWT
                     PTNLGDTQDGNVSLEYALVFSKNVPSIDLFKRLGAKNVEKWARRLGFTTKIIPDDALA
                     LGASCTKVDEMARAFTIFARNGKWWPRPVGQEKDWIYVRRILDRDGNTIEDNTAIEDP
                     QLGLGDRFDRFAVLAGIQPKEAIPARSAFLTSKLLASEVEFGFANILRATGLIAAGKT
                     GTSSATHDNWFIGYTSKFTTVVWMGDDKKQRALGKKDAAYMTVVPLWARYMYEAAKGY
                     PNPKIPWETPPGVKPGDRGDHSKGEKGPQMDLIWHSPKRDADTGADDRPPV"
     gene            34287..34745
                     /locus_tag="VFV99_01680"
     CDS             34287..34745
                     /locus_tag="VFV99_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058033.1"
                     /translation="MRLLWVFVVGIAAFGCKDKQPAKSADPAPKGTTPTTPGSPTSAA
                     VSQPRSAAPTLPADELFAKESTDSEWAPGAERAVRAVAPELEDLACKHDQCRATVVAT
                     NETELMAAVDKLQTPESLLGVPEAKSIKLTQPVQSGNGYTMTIYIRFDRE"
     gene            complement(34753..35406)
                     /locus_tag="VFV99_01685"
     CDS             complement(34753..35406)
                     /locus_tag="VFV99_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058034.1"
                     /translation="MALLARATSKNPTEWTDRELLKWVLRSDARGWSELVRRYRPLIY
                     RCITKVTLKYAPTLGNADLDEIYADVMMQLVRDDMHKLRIYNPARGTKLGSWIGMISV
                     NAAYDFLRCAGRRPLLDKVDGALDPHEECDRTPLDQLIEKERWDHLNGLLAEFTDKDR
                     TFVELYYQKGLEADEIAAEMQISLKTVYSKKHKIRAHLVRCLTQISGDSAIADLALA"
     gene            complement(35953..37425)
                     /gene="glpK"
                     /locus_tag="VFV99_01690"
     CDS             complement(35953..37425)
                     /gene="glpK"
                     /locus_tag="VFV99_01690"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX5058035.1"
                     /translation="MRHVIAIDQGTTGSTVLVLDEQLQVRGRGYKEFRQIYPKPGWVE
                     HDPEDIWTSVTTALAAAMQGIEPSSVAAIGITNQRETAVIWDRKTGKAAHNAIVWQDR
                     RTADRCAELKAQGKEARVKQLTGLTLDPYFSGTKVHWMLNNVPGLAAAARDGSLAFGT
                     IDCYLLWRLTGGAVHATDVSNASRTLLFDLKELTFTDELLELMGVPRALLPNVVPSSG
                     PVGTTKGVPGIRDGVPIAGLAGDQQSALFGQTCFAPGDAKCTYGTGAFILMNTGEWAV
                     QSKSGLLTTVAWKLHTGELRYALEGSAFIAGAAVQWLRDGLGIIKSASEIEELARSVP
                     ESGGVIVVPAFAGLGAPHWRPDARAAITGITRGTTKAHLARATLEGIALQNVDILRAM
                     ERDAGRPLTMLKVDGGAAANDLLMQFQSDVLGVEIARPELVESTALGAAFLAGLGTGV
                     WKDLDTVKATWREQRRFKPTTDRAWVKEHLTRWDAAVAKA"
     gene            complement(37413..38225)
                     /locus_tag="VFV99_01695"
     CDS             complement(37413..38225)
                     /locus_tag="VFV99_01695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5058036.1"
                     /translation="MALGEPFDLVGDGAIGVVCVHGFTGTPYEMRYLGDQLARAGFTV
                     HGLRLPGHGTRVEDLDQTKWSDWADAVEDAYDSMRLLCNQVAVVGQSLGGLLALHLAS
                     KRTDVAAVASLAAPLWLEGLSARVAQWAADGMLRRIRAIPKFYGSDVRDKRTKAENPC
                     YSAIPTRALAELAAFMRVVEGELSSIKQPVLVLHGEHDHTAPVACATRIAQLTCAARM
                     RILPRSYHLIAADVERDIVAAEVIDFLRRNVRTTDRTTDRPHEQSGDLACAT"
     gene            38340..39221
                     /locus_tag="VFV99_01700"
     CDS             38340..39221
                     /locus_tag="VFV99_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5058037.1"
                     /translation="MSEWKTRITKVQVVKPAKESGEACLVLIYPPGPDMGKRFALTRN
                     EIVLGRGADCDIQVDRDSVSRRHARVYRTGESWSVEDLQSTNGSYVNDVPVTKSVLRD
                     ADFVKIGAAIFKFLSGSGIEASYHEEIYKMTIVDALTGAHNKRYFLEFLEREIARCAR
                     YRRPLSLLMFDIDHFKQINDKHGHLTGDYVLREMSRRLLGRVRREELLARYGGEEFAA
                     VLPETDLTGARTFGEQIRRLVADQPFEYEGDTFPVTVSVGVATVEGEDIDVQAFIKTA
                     DDHLYRAKREGRNRVIG"
     gene            complement(39245..>39519)
                     /locus_tag="VFV99_01705"
     CDS             complement(39245..>39519)
                     /locus_tag="VFV99_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058038.1"
                     /translation="GITGRGYTGVTTIHDLTDATSYTFDSTDGSVVFGTEGSTCSKGV
                     LVFKQGSIYGALRFITINADNSVDIEYWIGQAGVTDFRNAPDAPPP"
BASE COUNT         6482 a        13000 c        13115 g         6922 t
ORIGIN      
        1 gagaccgtcg agcgtgcgca gcgcgagctc gcgcttgcct tcacggccga ggatgtcggc
       61 gatgtagccg agcgcctgcg gatcgagctt gtcgcgctcg agccacttgt tcgcgatgtc
      121 gcgcgcctct tcgagcttgc cgccatacga cagcgcctgg acgagcgcgc ggtgcttctc
      181 gcggctatcc gggttcttga cgagcgcgtc ctgcgcgtcg gcaattgcct tcgtgatgct
      241 cgcgctcgta ccgtcgaacg tgccgagcgc agcgttgcgc gtccacacct tcttcatctg
      301 gatcacgatg cggcgaccac caccgacggg cgggcgcggc atcgcgcggg gcatgtcctt
      361 tgccggcgcc ttcttctcgg ctttatcggc gggcttctcg gcttggatca caccgccctt
      421 gccgccccaa cgggtgccac cgccaccgcc ggcgtcgagg ctcatcgtca tctcttcttc
      481 atcacgcgcc gcgcgcggtg ccatcgaggc cttcgccatc gtcggcgcgg gagccggcgc
      541 attgtcggcc ggcaccggaa tcgtgcccgt cgccgcgacc tcgtcgatat cttcctcgcc
      601 ggtccacttc gcggccgact ggctgcgatc gacgccgaac gcatcgaaca tcgcctgcga
      661 ctcgagcacg agcagcgacg tctcgcgcga catcacgccg tagccttgcg acagcgcgac
      721 gatgcgcgag cggtcctcgc ctttgccctg gcgctcgagc tgatcgatcg cgagcgacgc
      781 ccacagccgc ggcacgaagc cgttgccgtt cgccgtcgac accgcgagct tcagcggata
      841 gcgctgctcg aacttctggc cggcgacctt gccgcgcagc acgacctcgc cggagacatc
      901 gccgttgatg cgcgctgcga tcaatacttc accaccggcg cggagcgtcg gcaggctcgt
      961 catcgtcgtc ttctccgcgg tcgtaccacc ggccgcggcc acttcggtca tgccttccgg
     1021 cagctcgacg gtcgcatcgc gtagcgacgt accatacgtc gattcgagtg cggccatcgc
     1081 cgcggtgccg accgactggc cgggtaccca cgcgaggtag ctaccgccac cgccgcgcgc
     1141 cgcagccgcg agcagtgccg agtcggcatc gctgccgaca ccgatcgtcg tcacgtggac
     1201 gtcgccggtg ccggtcgtcg acgcgatcgc cttctcgaca tccgcgacgc ggcggaagcc
     1261 ggtcgatgcg aaaccgtcgc cgatgtagag cacccagcgc tcgcgcgaca ggtcttttag
     1321 accggcgtgg cctgcacgga gcgcggcgac gacatcgctc gcacctgcag gcgtttgcgt
     1381 ccccagccag cctttgagct caccggcggc cgacgtcgtc ggtgcgcgca ggtcacccat
     1441 cgcgcggcat tcgctgtcgc acaccatcgc ggtgaaccga tcgcggcgat cgagatcatc
     1501 gaccatcgaa accgcgagct cgctggcgcg cgtgaaccgc tcgccgacca tcgactgcga
     1561 accgtcgatg atgatcatgt agtcgcgcgc cttcgattcg cgccagcgcg gcagcttcgg
     1621 cgatagcgcg agcaccgccg tcgggcgggc atcggcagca accaccttct gcgcgtcgat
     1681 gaccttctgg tcgatgccga cgttcttctt cgccgcgagc ttgtcatccg ggccagcagc
     1741 tgcaccgccg gtgaacgtcc acgcgcgcac ctcggcgtca ccgtcggggc ggtaatcgac
     1801 gacgaggtcg ccgcgcggca cgaagccgcc ttgctcgagc gtcatcgcgt tgacatcagc
     1861 gcgcgccgag tccggcttca gctcgtaccc cgccgcgcgg accgagccct gctgcgcccc
     1921 acgcacttcg acgtcgacct tcatcttgtc ggcgaccgtc gagccgtccg tcgaatgcgg
     1981 cagcggatag acatactggc gctgcggtcc gcgcggcgtc accacttgcg tgtacgcgag
     2041 cttgatcgtg cgcacgccct tcgccggaat cgggaagatc ttcagctcga agcgaccacc
     2101 gcgcttccag tcgagcagcg ccggatcttt ccacgggccg ggcacccaca cgatttcttg
     2161 ctggatcagc gggtcgcgct cgatgcgctt cggcgtcgcc ttgtcgatca cgccgcgcca
     2221 gatcttcgcc gcgcgctcct tgtcgacgaa cgcgccgttg atgaagccat cgggctcgtt
     2281 cttcatgtcg agcgcgaggc tgtcgatctg cgcgtcggcg ggcagcggga actgatacac
     2341 gccttcgagc tgcgtgtcgc tgtcgttgcg gaacgtctcg gtgatctcgg tccgcgcgac
     2401 cggtccggag atgcggacct tcacttcgtg gttcgcgagc gcgagattcc agtccttgtc
     2461 gcgcttctcg ccaggcttgt acgcgcgcag cgcgccgagg cccgacgtcg tctcctgatc
     2521 ggtctgcttc gtcttcggct gctcgatctc ggaccactgc gcgtccttga cgagctctgg
     2581 tgcggcgctg accgagagct tgccgttctc gatgatgcct tcctcaccgg cgcggacctc
     2641 gtctttcgcg ccgttcgcag cggtgagcac gatgccaccg cgcacgacct ggacggcggt
     2701 gaggttgtcg gcagcggtga cgctaaagcg cgtgccgacg acatcgacgc ggccgctcgg
     2761 cgtcgtgatc gacgcctggc gattgtcgac gtgcgcgaca tccgcggcga tgcggcctgc
     2821 gaccagcttc acgcgacgcg gctccttcgc atcgaacacg acgctcgtgc ggtggtcgag
     2881 caccatgcgc gagccatccg ccagctcgag cgacacgcgg gtgcgctcgt cggtgcgcag
     2941 ctcggcgccc gtcggcaaca tctcgccggc gcgtagtggc cgccagctgc gcgttgcgga
     3001 gtccttgatc gcgatgccgt cgccttgttc gcttgcggca cgcgagatcg tcttcacctt
     3061 gccgatcggt ccattgccgg caacggtcgt gtgcgacgcg tcatcgccgc ccttcttcat
     3121 catcgcgaca ccgacgacac cggcagcaag tgcggccgcc gcaccagcga caatccacag
     3181 cttgcgtttc gcgatcggtg taggtgcggc gatcggcttc tcgaccggtt tctcgatcgt
     3241 gttctcgttc gtcttttcga gcttcttctc gacaggtttc tcgtcgagct tggtcatcaa
     3301 ccgatcgacg agatcgtttg acggaacata gtcactgccg gcgcctgcaa ggcgagtcgc
     3361 aaggacggcg gcctcatgct tggcatcgcg gcattgatcg cagctcgcga ggtgcgaggc
     3421 gtgtttggaa atggcagatg cctcgccggc gacaagcgca gcgagatcat ccatgacgtc
     3481 atcgtgggcg gtacgcatta ttcgatctcc tcggtgggca tgaccgagcg aagccggtcg
     3541 agcgcgcggc tgatccgctt gcgcgctgcg gcttcgtcga gcccgcaggc ctccgcgatc
     3601 tcgcggtggc tcaggtcagc gacatagcga agggcaagcg actcgcgctc gctcggcttg
     3661 agcttctcga gcgcgtcgcg caatacacgt gcgcgttggc gcagcgcgaa tgaatcacgc
     3721 gcttcgcctt ccgtaggcac gagctcgagg agctccttgc cacggcgtcg agtctccagc
     3781 atgtgcgcac acacgtgccg cgcgatcccg cacaaccacg ccttcaccga tccttcagcg
     3841 cgatacgacg ccatcgcgcg atgtgcgcgc aacagcgttt cttgaacgcc ttcgtcggcg
     3901 tccgcctgag aaccgagcag ggccatacac aaccggccga gcacgacgtg gtgtgtgcga
     3961 gcgcacgcag cgagtgcgtc gcgatggcga ccgtcgtgga tcatctccac gatcgggtcg
     4021 tgagcgacgg cgttcgttgc taacgcttgc atgtcgcccc ccacgtgcgc tgccaccgag
     4081 ctttcgtgac cgccttttcg cgaaaactcg gaagtgcctg aagttgcagc gccgtcggtg
     4141 agccccatgg gcgatccaac gcgcgaggcg cggccggtgt ctacgccgct tcgtctagtc
     4201 ccctactttg cgacgcacgt gtgggtgtcg ccctccgctg gctgcttcaa gcaaccatcg
     4261 atttgcccgt cggggcactc gagtgcgact tcctgcgtgc agggctgact atcacccgtt
     4321 gtggtctttg tcgcatcgct accactatcg ctgccgctgc cgctgccgct gccggtgttg
     4381 gtcgtcgtgg tgtcctgctt cttgctgctg ccgtggccac agccgatcgc gaacgcacca
     4441 atgatcatga acgaaatgag cttggtcatc tgtctcctcc agggcgatca acgaggagca
     4501 ccgacgcggc ttacagctag ccgatgaacg cgcgcgtgat cgcgaatgca atcaacgcca
     4561 tgacgatcgc gccgaccacg agcacccatg gtttcagttg cggtttgcgc gtacgaccaa
     4621 agcctgcaac ctgcggcatc gacgtcgcgc gcggtggaaa cagcggttga tcgaacgagt
     4681 cctgcgcggt cggcgtcgca tcacgcgttg gaatctgcgg tgcgatcggc ggcgcccaac
     4741 cggcataacc gcccgcgggt tgcgacggcg cgaacggtgg tgcgccttgc atcggcatcg
     4801 caccgcccat cggcatgttg ttcatgccca tcggcaaatt cgatccgagc ggctgcggtt
     4861 gtggtgtcgg cggcagtggc gccattccgg gtacggagtt gccggtcatc tgcggcggca
     4921 tcggcaatcc cggcagcggc ggtcccggcg tcgggccgcg gccacgcaac ggcatcggtg
     4981 gcggcactgt cgacggacga ttgctgcgcg acgggctcgc cgtacgcatg ccaggcaccg
     5041 gagtgcggcg atcacgcggc gcgatcaagc gcgtgtcttc gcccggcgtg ttgaatggcg
     5101 gctggtagcc gtcgtcgagc ttgccgagat cgagcggctc gcgtgcgcgc gtcttttcct
     5161 cgtcgctgtc gctgtcgcgg cgcgcgcgac gcgcgggtgg cgcaccttcg gcggtcagct
     5221 cgccagaatc gcgaatcggc gctgccgtgc gcagcatgcg catctggtcg cccgagagat
     5281 tcgcgagtcg cgtctcttct tcctcgaagc ggtcgatcac caagcgcgct tgcgagatgc
     5341 tttggtcctc gcgcgccgag aacgcatctg ccgtgcggat gcgaatcacg gttgcttcgg
     5401 cgccgtcgaa gtcgaagcgc cccgattgat cgcgcggtgc gatcaccttc tggctctggc
     5461 gctcgagctc ctcggtcgaa tcgatcagct tcgccagctc gctcgccgga tcaccgacga
     5521 cctgatcgag cgaatagcgc agcgagcgca gcttctggcc gagctgcccg gtcgtcgcgt
     5581 gacggcgcgt cttgtccttc gcgagcgcgg tcatcaggat ctgatcgatc tccggcggca
     5641 gtcggccgtc gatctcgctc ggccgcggca cggtcgcttc acgcaccgag cgcaacgcgg
     5701 cgagatcgtc gaggcccgag aacaatcggc gattgcagat cagctcccac atcaggatgc
     5761 cgaccgagaa cacgtcgctg cgcggatcga gtggctcgtt gcgcgcttgc tccggcgaga
     5821 tgtacgcgaa ctttccgcgc acggcaccgc cctgggtcgc gtcttgctcg gcacgcttcg
     5881 cgatgccgaa atcggtgagc ttcacttcgc cggcgcgcga caacagcacg ttcgatggcg
     5941 acacgtcgcg gtgaacgagc gccatcggcc tgccatcggg accgcgcgac gtgtgcgcgt
     6001 gctccagcgc ttcgcagatc tccgcgccga tatgcagcac gagatcgaac ggcatgcggc
     6061 ggcgtttttg ctgttcgagc gcgcgctgca cggcgccgag gtccttgcca tcgacgaact
     6121 ccatgaccag gaagtactgg ccgtcatcgc cgaggccgac atcgaagatc tggacgatgt
     6181 tgcggtgctt gagcagcgac agcaccttcg cttcggcgat caacagctcg acgaaccggc
     6241 ggtcctgcga caggtgaggc aggattcgct tgatcgcaac gggcttctcg aagccgccgg
     6301 cggccacggc cttgccgcgg aaaatctccg ccatgccgcc gacgcctatg cggtcgagca
     6361 gctcgtaccg ggtcacgccg catcaggtta ccacggttaa gatgtcctac atggatcgga
     6421 tcaagcggct tctcgacgag gcgctcgcca atggcctggg ctccgcggcg gccgtctccg
     6481 tcggtgatgg aggccacgaa gttttccggt acgtcgcggg agtcgtacgg cgcgtgcccg
     6541 atcgtggtca gcgcgtcgag gaatcgacgc ggttcgacct tgcgtcggtc acgaagccaa
     6601 tggccaccgt cgcgatcgcg atggtgctcg tcgccgaacg ccgtctcgat ctcgacgcac
     6661 ctgtgcgcac gtacctgccg ggcgcggcga ccaccgcgac cgtgcgtcag ctgctcggcc
     6721 acagcgccgg ctgcgccgcg cacgtggagt tcttccggta cctgcgcgca acgcgcccgc
     6781 agaatccgcg cgaggtgctc gtcgagcgcg cgatgcgcga gccttgcggc ccaccgggaa
     6841 tcgacgccgt ctacagcgat ctcgggttca ttcagcttgg cgcggtactc gagcgcgcgg
     6901 ccgagcagcc gctcgagcag gcgttcaccg agctcgttgg cgaaccactc gcgctcgccg
     6961 cacgttacgc acccgaggcg ctcgtcaact gcgtcgcgac cgagctcgat gatcgcggtc
     7021 tcgtgtcagg gctcgtgcac gacgagaact gctacttcgg cggtcgcatt gcaggtcatg
     7081 cgggtctgtt cgcgaccatc gtcgatgtcg ctgcattcgc gcgcgcgatc accgataccg
     7141 cagctggtac accgcgcggc cggttcacga ccgatgtcgt cacccgcttt ttcaccgaca
     7201 caccgacgcc aggatcgact tggcgactcg gttgggacac gccgtcgtcc acgccgggcg
     7261 tgtcgcaggc gggcgatcgc tggccgcgca cgggcgcggt cggtcacacc ggtttcaccg
     7321 gcacgtcgct atggctcgat ctcgcgaagc gtcgctgggt cgcgctactc acgaatcgcg
     7381 tacatccgac gcgccacgga gataccgccg atcgcatcaa ggccttgcgc cgcgcgatca
     7441 acgacaccgt cgtcgagacg ctcggctgat ccgcagcatc caagctgcat gcggagacgc
     7501 aaggccctcg cgctggtcgg gctactcggc ggcgtgttga tcgccgcgct cgttggttgc
     7561 gccttcgtga tctggcgcgc gcctgacatg gcagcgctga tcgcgttcgg agcgacgtgt
     7621 gcggccgcga cgacgtgcat gtcactgcgt cacttcggct ggttgttcct gatcgtcgcg
     7681 gtcgtgtacg gggtgctcgc gatcgggctc tacgcgctgc acctgctcgg cctgccgttc
     7741 gccgccatcg tcaccggcgt cgcagtgctg gtcgtgatca tcctcgcgac gatgatgtgg
     7801 gcgctgatcc cggctggcac cgaggacgat ccgtacgacg actagtgacg ccggccgttc
     7861 gtgcggaagc cgttgcgctt ggacttcgac ttgccggctg cgcgcacgtc ggcgccggtt
     7921 gcgccgaaca tcggatcgac gccgcggtcg gcacagaccc acaacgagac gaggatcgct
     7981 agatcggcga gcgcgcgccg gctcatcgac gccggaatca cgagaccgta cgtctccgat
     8041 acgaagcgca gatcgcgaag caagcggccg acctcgtcac gattgacgtc accgtgccag
     8101 tacacgacgc gcgtcatcag cagctcggcg tgccggcgca agaactcgcc catcgacagc
     8161 ggctgcacga tgcgcaccga cggcggcttg cagatctcga ggagatcttc gaggtactcg
     8221 ttccagtgca gcgcgagccg gcggttctcg gggcgacgta agaacttcac gcgctcgacg
     8281 gagaagtcgg ccttcaccgt cgtgcggaag cgcggcgtga ccgccatcag atcgtaaagc
     8341 accggtgggt cggtcttcga gtaatcgacg tcgtggtact tccaccacgt gcgcagctgc
     8401 ggccacgtgc gcagcgacag cagcggatca tcgttcgagt cgacgatcac gaaccggcga
     8461 tcttcggcgc gcagcaccgt gatccagtgc gtccactcgt ccacgcacag gagcactggc
     8521 gtttgctcgc ggaggtagtg cacgagcagc ttgcgcgctt gctcgggatc gcggcgacgt
     8581 tcgaggacga ggtcgcattc gaactcgcgc gcagcgcggg caagctggat ctcatccgtg
     8641 cccgaccacc agtgcgtctt cgcggtcgat gcaagctgct tgacgtcgac catgcgaccg
     8701 agcgcgagca gggcatgctt gagcgcgaac ggaccacacg tccattcgtt cggttgcggg
     8761 tagaaacccg gaccagcgat accctctgcc acggtgcgac tctagctcaa acgccgggcc
     8821 gcgctgccct gccgagaggc cgagaaatgc taattatcag gatgggttag agtccgcgag
     8881 atccggatta accgcatggt tggtcggcca ctgccagcgc cggcccacct ggacagagtc
     8941 cgccggatgc tgctcgacca tgcgcctcca gcggtcgaat gcgagcgcgt gctcgccgca
     9001 acgagcggcg cgctggatcg cttcgatcac gaagctgcgc tggcgctcga agtgctcggg
     9061 cgtccacgcg atatcgctga catcgagcag ctgagtaccg gcgtcggcgc ggctatcgag
     9121 ccacagcacg agctctactt cccaccgcgc ttcggcaaac ggccgcgcgg cggcggcggt
     9181 ctcgcgcaac atcgtgagcg cgacatcgtc ggagacgaat agctccgcgc tcggcccgag
     9241 ccggtggttc gagaatacga ccatgcacat tcttacgtgg tcggtgtgac agccgattcg
     9301 cgcatcggta acgggagcac gcccgctctc actacgtcat tacgaagccc ctggtcctcg
     9361 aggccctgaa gcgctcgcgc gcaggtcgca cagggggcga cgcggtcgcc gggggcgaac
     9421 agcgccaacg gaccgtccgc ggtgacctcg atcgcacctg gaccgaggtc ggcgagcgca
     9481 gcgcgtagtg cgtcgccatc gagggaacct ggtggaatac ccaggcgttg ctcgcacacg
     9541 ccgagatagc cggtgagcgc agcggtgatc gcgcactggc ccggtggcac gaagccgtcg
     9601 agccactggt tgtgggcgat tcgatcgatc cagtcgagat agaacgcggt gaagctcggc
     9661 cggatcggct gcaggatgcc gagctgccgc gcgtcgagcc agacttgtcc ggaggccgga
     9721 ccatcgagag ttagtagtgc tgcgtagccg cagccgaggt gactgatcgg cagtgcacgc
     9781 tgccacgcgg tgacgccggt cggcgcatcg atcggtgcga cgcgatcgac gtagatgagg
     9841 ccgtaatagg gaccgactcc gccggccgaa agccgggtta cgtattcgag gtagtcggca
     9901 ggcaattcga catcgagcat gccctcggcg attggtggcg cgaggtgata gtgatgctgc
     9961 gcggcgccga agcgcctgaa gcgcggatca gatgcagcga gcccaccaag aatctcgcgc
    10021 aacctggtga tcagctcact caccgatcat tcaccgatca cggttaccga gacatgacgc
    10081 cgatgcggcg acgtgcggtg ctcccagaga tagaggcctt gccacgtgcc gagcgcgagc
    10141 ttgccgttcg cgatcggtag gccgatcgac gtctgggtca gcacggaccg gacgtgcgcg
    10201 ggcatgtcgt catcgccttc ggcatcgtgg gtgaacatgc gatcaccatc gggcacgagc
    10261 ttcgcggcga acgtctcgag gtcacggcga acgctcggat ccgcgttctc gcagatgatc
    10321 agcgacgcgc tcgtgtggtg gatgaacacg gttgccaggc cattttgcgc gctacttgca
    10381 gcaacaacct tcgcgacttc cccagtgatg tcataggtcc cgcggccttt tgtcgcgacc
    10441 gtcagctcgc ctcggtggaa catggcgacg gcagtagagc acagacgtga caaggtgggc
    10501 ggcgagcgcg atactgcggc cgtgatccga cgctcgctcc gttatgccga agagctaacg
    10561 gacgccgtca agcagcagct cgcgcttgcc tacacggcaa aggacttgct cgcgttcgga
    10621 ctgcgctcgc gcatgcgcga gggctactcc aggaaggact tccgctccga cctgatggcc
    10681 gcgctcgtgg tcggcgtcgt cgcgctcccg ctatcgatgg cgctcgcgat cgcgtgcgac
    10741 gtgccgccgc aacacggcct ctacacggcg atcgttgccg gcgtcgtgtg ctcgctactc
    10801 ggcggcacgt acttccaggt caccggcccg acggcggcgt ttgtcgtgat cttgttgccg
    10861 atcgtccaca agttcggcgt cggaggcttg ctggtcgccg gcatgttggc tggtctcatg
    10921 ctcgtcggca tgggcttggc caggctcggc aagctcatgc agttcgtgcc gcatccagtg
    10981 acgagcggct tcacgatggg catcgcgatc gtgatcggcg tcctgcagct caaggacgtg
    11041 tttggcgtca agttgccgcg caccgagggc gtgttcgagt acttcggagc gctgatcgac
    11101 gcgcgccatg ccttcaactt ctgggacgtc ggcgtcgccg tcatcacgct cgtgatgctg
    11161 ctgatgctac cgaggatcat caaacgcatc ccggtgccac tgatcgcact cgtcgtcgcg
    11221 gcgctgctcg tggtcgtcgt cggccatttc gcgcccgaga gctggggctt ccacgtcacg
    11281 accatcgggt cgaagttcac gtacccgctc ggcggcggtg aggtcggcca cggcattccg
    11341 ccgctgccgc cgcttcccgt gctgccatgg caggtcgccg atccgggcca tccaccgttc
    11401 gacctcaacc tgcaggtcat ccgcgagctg ctgccgtcgg ccttcgcgat cgcgatgctc
    11461 ggtgcgatcg agtcgctcat ggccgccgtc gtcgccgacg gtatgggcgg cagccgccac
    11521 gatccgaacg cggagctgat cgcactcggc gtcggtaaca tcatatgccc gttcttcggt
    11581 ggcatcgctg cgaccggcgc gctcgcgcgt acggccacga acatccgcgc aggtgcccgc
    11641 tcaccgctcg ctgcctcgat gcacgcgatc ttcattctcg cctgcacgat cgcgctcgca
    11701 ccgctcgttt cgtacctacc gatggcggcg cttgcggcgc tgttgatcat cgtcgcgcgt
    11761 aatatgtccg aggcgcgcca cttcttccgg ctcgcgcgca ttgcgccgaa gcacgacgtg
    11821 ctcgtgatgc tgacgtgctt cacgctcacg gtcgtgttcg acatggtgat cgcggtgacc
    11881 gtcggtgtcg tgctcgcagc gctgttgttc atgcgccgga tgtcggtgct cacccaggtc
    11941 gagctcgaga cgccgactac tctcaagatc gatgtaccgc ctgggattcg cgtctacgag
    12001 atcgccggcc cactgttctt cggcgccgcg aagagcgcga tggaggcgct ccacacggtc
    12061 ggcgacaagg accacacgta cgtcctcgac atgcaacacg tgccggcggt ggatgcgact
    12121 ggcctcgtcg ccatcgagtc catgatggat cgtttgcatc gctcgaagat caagatcatc
    12181 ttcgccggtc tgtcgacgga gatgagcgac ctgttcgcgc gcgcgggcat caagcgcgag
    12241 ctcggcaagg tcgcgtatgc gccggatgtc gagacggccg tgagcatggc gatcgtgcac
    12301 gcggcgcgca ccggccgccg ggcagacacg tcgccctcga tgcggattcc gaaggtcgac
    12361 gactcgggtc agccggcggc ctagcgcgcg ggcggtccgg cttcgagaat cgcggagccc
    12421 gactgatccg aggacacgag gatcgcgaac ttctcgacgt gatcgagatc gttcgtcgat
    12481 aggctcgcgt agtcgaacca accgcgcagg tcggtgtagc cgtccttgta gaaagcgacg
    12541 gcgccaccgt gcttctgcgc gtagaccttc acgtacgtcg cagcaagcgc cgcatgatcc
    12601 gacgcacgct ggacgcggac ctggccgtac tgatgcgcga cgtacgtcgc gagatcgttt
    12661 gcgtagtgga tgcgtgcctt gcgttgcccg acgccgacgg cttcgacgac gacattcttg
    12721 ccgcgcagcg ccggcggcca cgcgacgcga tgctcggccg gcgggttcgt caggtattcg
    12781 cgatggccgg gctcgatgaa cgagaaccgc gacacgtcgc tctgcacgaa cggctggcgc
    12841 gagaacaaca gctcgacgtc catctcgaag aaccgcagct cgagactggt gacgtgctgg
    12901 ttgcggatca gcacgccctc gcgatcgagc gcgatgtcga acgtcggctg tttggcagcg
    12961 agctcggcct gctgctgatc gcggctgagc gcgtcgacga cgctcggtgc tgcgccggtt
    13021 agctcgtcga gcatgttcgc gagcgcggag aacttgtgac tccagcgatc gacgcgcaac
    13081 tcgcgccagc gcgccgtgag ctcgcgcgcg cgcgtgatgt cgccgatcag gcacgaggtg
    13141 tacgcggcga gataatcatg ctgcatgcga tcggcgagct gcgtgacgtc gacacgaccg
    13201 agcgcggcga gtgcaccgtc cgcacgatct tgcgcgagca agtagtgcgc ggcggcgagc
    13261 atatcttcgc tcgtcggcag cgggcgatgc gcgaccagct cgaggaagcg agtgtactgc
    13321 gcggcgaggc cgtcgttgag gatgcgcagc ttcgggccga gccggtgcgc gcgcgcgttg
    13381 atcagcggcg cgagctcgag atgctcgtag ccgtcgagtg cttcggcgtc gaggccgagg
    13441 acgggcatgt cgagcaccgg gcccgcgccg agcagtgcgg cgccgagcgc acgcgcccag
    13501 atgtgaatgc gcggcggatc gcggtggagg agcgcgtagc cccagaggac gggggcgtag
    13561 acgcgacgcc gttcgagcgc gccgaggatc gcgtcgtacg ccgcgcgatc gcgcaggcgc
    13621 cacgcgacgc gttcgagatc gacgtcggcc aagctctgct gatcgaggaa cgcgacgacg
    13681 tcggcgaccg atccacgctg cgagatgtgc gcccacgact gcggatcgag cacgtcgccg
    13741 ccgagggtga cctcgagcac gcgacccggc gccgccgcaa cgatcgcgcc accgcggctg
    13801 acgtgcaccg ggaagtgtcc atagcgaccc ggcgccggga agtagaacgc gtactcgtgt
    13861 ccgtgcgtgc cgtacggatc gagcgcgatg tcgagcgtct gggtcgccag cgcacccgcg
    13921 agggaaatgc tgccgcgcgg aatctggatc agcgccgcga tccgttggcg cgagctcgtc
    13981 gggttcgcga gcacgacctg acacgtgtag acgacgccgg cgatgaacga gccctcgatg
    14041 tacttgtcga cctgctcgcc atcgacgtag cgatagcggt cgtcggtgcg cacgtagctc
    14101 tgcccgacga cgagtggcgc gccggtttcg acgagctcac ccggcacgag ttgcgatgta
    14161 ccggcgagtg tgttcgcggc ggccgtgatc gtcaggcgcg ggccatcggg cgcgagctgg
    14221 tgcgagcccg cgacgaacgg caggtcgacc agtgcgagcg cacacatcgc ctcggcgaag
    14281 ttgttcgtgg ccaggccgag ccactgcgac agaaatacgc cgtcgcgatg caacgcgaga
    14341 tcgcgccaga ggcggtttgg ttcgatcagg tccgcgccgc tctgctgcgg tgtctggtgc
    14401 caccagttat tctcggccca ctcctgcgtc ttgtcgacga cgcggtacat cggcgcctcg
    14461 gcgcgacggc gctcgaggtc catgtcgagc gcgtcgtatt cctcctcggc ggcgatgctc
    14521 ttcgacatct tcttcgcctc ggcacgtcgc ttcggcgcag gcgccggcgg cgcttgtgga
    14581 gcagccatcg ttgcgacctc ggccatcacc atcgtctggc gcgtcgtgac cgcatgttgc
    14641 tgtagagtct gcagctcgct tccgccttcg agcgtcgacg caccgagcag cgcatcgatg
    14701 atccgtgtgt cgagctcggg attcggcggc tgcacgccga cttcgtccgc gagttggcgc
    14761 acgagtgcgg cgtcaccgag cagtcgctgc gcgagcaggg cgcgctcgac ggcattgagc
    14821 cgggcgagcc gcatcggttc gaggtatgag gcgagatcga cgtcgagcag ccagtgatcg
    14881 aggaacgtct tcgtgcgctt gtggacgagg tagggccgca cgaccgcgtc gaagaacgcg
    14941 cggtccttgc agtagaggaa caggtgcagc tcgtggcacg cgtatttcga gtacagctcg
    15001 cggcgctcgg tatcggcgag agtgtgccag cgcgtgacga agctgaattc gcgtagcgtc
    15061 gcgtcgtcgc gaagcgcgag cagataggca tgcgcgcgct cgacggaatc gatcaggtgc
    15121 agcttcgcgg tcgcgaggtc ctcgatcacg atcgcattgc ccggcaacag cggacggatc
    15181 gtcttgtgct gggtgacgtg gctctccgga gcgagcgcga tgtgcaggcg caggtcgcgc
    15241 gttgcgagtg gcgactcggc gagtgcgaga tgacggcggc tggtgcccgc cggatcgtcg
    15301 acgatcacgc tcacgcttgt cgcgttgccg agctcggcga gcggaagcgt cagcgcgcca
    15361 tgtgcatcga cggtgagatt cgcgaatacg cgtgcgggct cggcgagaaa gtcgtaaccg
    15421 aggtacgccg attcgtcgac ggcaggttcg ccccgcgctt gcatcggtcc gccataaccg
    15481 gcgccgggtg ctcctccggg cgcagcggcg aacgcacgtc cggccgccgc gaccgcgacg
    15541 tccgtgcttg tcgtgcgccg cgcccatgga ttgagcaaga ggctcggctt gtcgagcaac
    15601 aggctcggga agcgacgcgc gttgcgccgc tccagcacgt agcgatactc gtcaccgagc
    15661 tcgcggccgg atacatagag cgcaccgcgc gcgcgatcga tgcgatagag cggcgtgcgc
    15721 agccggtact cgggcttcgc gaacggcgca gacacgaagc gtgtcgcgat cacgtgcacg
    15781 cgggtccgcg acgacgctcc aacgacccgc acgtgcaacg catcacttgc ggtcagctcg
    15841 gcgatcaccg gtgcgagcct cgagacgccg accgtctcac ccggtgccag gatatggccg
    15901 ctgatctcgg tgcccgcggc gacgcggatg atcacctcgg agaggccggg cgcgcgcacg
    15961 gcgtattcac cgacgagcag gccgcgcacg acgatgccgc cggtgagcgg ctcgagcgtc
    16021 acgttcggat gccgcaccgg atatccgccc cgcacttcga caatcgacgc tcggctgatc
    16081 acgtcgtgcg catcgcggtc gggcggcaac gcgatcgcga tctcggtgtc gggcgcgacc
    16141 tggatcgtct gtgcgggccg gccgccgctc gtcagccacc aggtttgcgg aacgctgccg
    16201 aacgtcgccg tgatccgctc gatgccgggc agctcgccga gctcgcagcg gccgtgcgcg
    16261 tcggtcgcga gctcgacgtt cagctgggtg cgcgcccagc ggtgaacgag cgtgacggtc
    16321 agcgagcgct gcgcacgggg ttcgccggtc ttgcccaacg ccgacagcac ccagccactc
    16381 gcggtgtgcg cgaggtagag cgcgtcggtc gcgagcccgc catgcatgct ggcgagactc
    16441 gcgcgctgga ctgccgacag gtcgagatcc cgctgctcgc tgacaacgcg tagcttgccg
    16501 ctgacgcgca ccatgatctg gtcggtcgct tcgccgacgg gaatgtcgag tacggccgcg
    16561 tcgtcgtcgg agagcgtgag cggctgctgc ttcgtcgtcg gcaccccgtg gcggtcggtg
    16621 agcgtgatct cccagcgcgg ctgttcgagc gccgcgagtg agacgggctg gccggacacg
    16681 gtgagctgca cgcgcgcgat tgcatgcgcg gtcgcgccgc tgatcagcgc ctggcgatcg
    16741 acgtggagat cgagcgtgag gtcgtactgc tcgcggacga gatcgaggta ctggaccgtc
    16801 gcgatgtcgc cgcatgacaa cagcatcggg gtgcgcgttg gcgacgtcga gaacggcacg
    16861 acgaacgtgc cgttcggatc ggggatgtat tcgcgatcgc cgatccacgc gcgcgtatcg
    16921 gcgcgcagcc ggccctgttc gtcgacgacc gtgaccgcgt gaccggcagc tccgatgcgt
    16981 gcgacgtgtc gcaagcgccc tttgtggatc accgcgcggc tcgacatgcc gttgccgatc
    17041 agatcgatga cgtagctgcc gccacgcgtg cacatcggca gctcgatgcg gcggcgcacg
    17101 cggcgaatcg gcggctcgct gtagcggagc acgagctcgt gactcgcggc gagcccgtcg
    17161 aggtcgacgt cggtcgagat ttctttgcgg ttcgtctgga agtacgcgag cggatcgatc
    17221 cggaacacct tcacgacgag ctcgggaacg ttcttgacgt cggcttcgag aatgatcggt
    17281 tcgtcgacgg caaatcgcgt cgggttgtgc aagcaccata cgagatcgac gcggtcgcgc
    17341 aacgcggcgg cacgcgtcgg accgagcacg agggtcgcgc gatcggtgtc acccgcaccg
    17401 ttgagcagct gcgcggtcgc gatctcgccg tcgagccacg tgcgatcgag ccactgcgcg
    17461 tattgctcgg cgtcgccccg cgtcacgacc tggcgctgca ggtaatcgcg cacgagcgct
    17521 tcgtcgtcac cggccggcgg caacccggtc acattgcgcc agtcgacacc acgcgccgcg
    17581 atctcgtcgc tgcgctttcc gttcagccag tcgcgcgcga cgtacgcagc cgagcgcggc
    17641 agctgcagat actcggcgaa caggccggaa tcgacaggcg cgttgcgctg gcgcagcgta
    17701 tcgagcaagt gccacagcac gtgctccttg agcccgttgt tcgtggcggg cagaggacgc
    17761 aggaactgcc acagctcgcg cagatacgcc tcgcgcgcgt cgcggtcgag ctcgatatcg
    17821 acgccggccg gcggccgcat ccggcgcacg acggcttcga cccacgcgcg gttcgtcagc
    17881 agcttggtgt cgagcttcac gagcgcgtgc agttgatcga gcgtgagctg gtgatggagg
    17941 ttgagcgagc cgaagccacc cgagccgcgc gcttcgagat cctcgtggat gagcgtgacg
    18001 agtcgctcgt gtggcgtgtg gccgatccgc gatagcagcg cgcggcgttg cgtggcgtcg
    18061 agcgtgcgat cgagcagctc gtagaggcct tcgtcggtga cctgcgagag gtcggtccag
    18121 ctcatcgctt cggtcaacag cttgtcgccg gtgaacgcgc cggccgcgag cttcgtcggc
    18181 cgcgacgggt cgacatctgg ggcgtcggct tcgtgccagt gcgagacgcc gtagaggtca
    18241 cggatgtcgt cggcggcggt atcggggctc gtgccgagcc ggtaccagaa ctggcgcagc
    18301 cgcatccgcc gatagcgctc ggtatcgccg tgccgctcgt gccacgcgtc gagaattact
    18361 tgtgcttcgt cgaaggcccc gcggtgctgc gcgtgcaggc accggtagta gtcgtggtct
    18421 tcgcttcccg gaagtagctg cgaaagcgcg gccgcacgat tgtcagccag ggctagcgct
    18481 tcgagggtct ccaactccat cgccggagcg tatctggtaa cgtcgacacg atgtcacggg
    18541 tgctgctgat cctcttgctc gtcgcgggag tagcgtcggt cgacgctgct ccgagcaaga
    18601 gcgacgccgc cgagacgaag acgaagaaga agaagcgcaa gaagaagaaa gagaaggacg
    18661 atcgcacgcc gaacaagcgc acgaaggcga acatgccgcg cgggttcgtg tggccaccgt
    18721 cgaagacgat ggaggaagcg gccgacgcat gcgagctgca gctcgacgag ctcggggtcg
    18781 catacgagac ggctgcgcca gcgggcaaga tcgtgcgccc gctgaagatc agcggcgaca
    18841 tcgcaggcat cacgtacacg tcgctgaacg gccggaacca gacgttcgac tgccagctcg
    18901 tgctcacgct cgcgaacttc gcgccgaagc tgcaagagct cggcatcaag gaagtgaagt
    18961 tcggcagcgc gttccgctac acgaaggtgc gcgtcggcgg caagacgaag aacgtcctgt
    19021 cgcgccacgc gctcggcatt gcgctcgacg tctacgcgtt cgtcgatagc gagggccacg
    19081 aggcgaacgt ccaccacgat tacaagcgcg gagatgagct gctccacgag ctcgagcgca
    19141 ccgcgaacaa cagcggcatg ttccgcatct tgctcacgcc gcgcaacgat ccgaagtcgc
    19201 actacgatca cttccacttc gaagcgaacc ccgactacgc gaacgagctc acggcggaga
    19261 agccggcctc atgaagttcg ccgcgctcgc gtgcgcgttg tttgcgtgcg gcggcgcggg
    19321 cgacgacgac atgatgggcg gccccgacgg tggcgtgacc ggcggcgacg gctcgatgac
    19381 gacggacgac agcggcatgc tgtgcatgaa gccggcgctc gaccagccgt ggctcgcaaa
    19441 cctcgtcagc ggcgcggtga ccgggctcgc aggcgcgccg cgcttcacga acacgcagcg
    19501 ccagacggcg cgcacgttcc tgcagaacca gctgacgcag atcggctgga caccgcagct
    19561 ccacagctac aacacgggag ccaacgtcta cgcgacgatt ccgtcgacga ccggcgagac
    19621 gaagcagatc gtcgtcggcg cgcacttcga caccgtgagc ggcagtccgg gcgcgaacga
    19681 caatgcgacc ggttcggccg cggtgctcgc agtcgcgcgc tacctcaagg acatgaactg
    19741 ccgcaaattc gccgtcaccg tcgtgttctt cgacgaggaa gaatccggcc tgttcggcag
    19801 ccgcgcgttc gcgcacacgt tgcaggtcgc gaacgtgcat gccgtgcata ccgtcgatca
    19861 ggtcggctgg gatcaagaca acgatctgcg tttcgagctc gagctaccga ccacgacgct
    19921 cgagaccgag taccgcgccg ctgcgcagac gctcggcgtg cccgtgacga agacgtcgac
    19981 gcagggcacc gatcaccaat cgtttcgcga gaagggattt ccggcgatgg gcctcaccga
    20041 ggagtacgtc ggcaacgaca cgtcgccgta ccgccatatg gcgcaggaca ctgccgcgac
    20101 cgtcgagcag agctacctcc agctcggcag caagctcgtc gcgctcgccg tgatgaacga
    20161 gctgtagcgc ctagcgcccc ggtttgctcc gcagcgggcc ggcgccatcg cgcggcatct
    20221 tcgacgcgat cgtctgcatg atccacaacg tacgcgtgac gcccggcgac agcgcgggtt
    20281 tgccgctcgt catcagcgcc accgcgatgt cgcgcgacgg atcggcatac acgaacacgt
    20341 tcgtgaatcc gacgtggccg aacgcgcgcg gcgtgttgag gccgtagagg ctggcgacgc
    20401 gtgcgccgag catgaagccc atgctgtagc gaatcggcgc gccgatcgtg aggtcgagct
    20461 cgaagaacgt ctgctcggcg atcgcacgct ggatcgtacg ggtctcgaac acgcgtacgc
    20521 cgtcaagctc gccgccgttc agcaagagct gcatgaaccg cgacgcttcg ttcgcggtcg
    20581 caacgatgtt gccggcgggc acgacgctgg tgaggaaacg cggatcgttc gagatccgca
    20641 cggcctcgtt gaagctcacg ctgaacagcc gcttcagcat cagcgacagc ggcggcaaca
    20701 ccggcggacc ggtgaatgcg ttactcgcga cctcgcggag gcgcgcgggc ggtacgccgt
    20761 agttgaacgc gtcgaatttc agcggcgcga gcagctcgtc gtgcatcacc tgacgcaacg
    20821 gcttgccggt caccgtttcg atcacggcgc cgagcacgaa gccgccggtc agcgcgtggt
    20881 acgcgagccg ttggccggca cgccagcgcg gcttctggcg cgcgagcagc gagatgatct
    20941 cgttcggtcg ctcgagcagg ctgacgtcgg cgaactgcgg cggaatattc gggatgccgg
    21001 cgcgatgcgt gagcacgtga cggatcgtga tgcggtgctt gccgtgctcg ccgaagccgg
    21061 gcaggtacat cgcgatcgga tcatcgaggt ggagcaaccc gcgctgatcg cacaggtgca
    21121 cgagcatcgc ggtgaccatc ttcgacgccg agaacatgtt gaacagagac ttcggcgacg
    21181 cgatcacctt gtgggcatcg ggcggatcga cgggcgcgtt acccgtgatg tggccgatcg
    21241 agcgatcgaa cacgacttgc ccgcgccgcc gaatacacag cgcgagtgcg gggtagaggc
    21301 cggtgcgata ggcgccgatc gtcgcgcgcc agatcgcatc gatcgtcttg cgatcgagac
    21361 cgacgctcgt cggatcgact tcgggaccac gatccgccac ctgctcgggt ggcgcggtgc
    21421 gcaccaacgt ctcgacgtgc gaccgcaggg acaacatcgc tgccagcgta gcagaattca
    21481 taattgcaag tgaattgcag gtgcttgcac gtgctcaggt gaaggcgtac agtccgagtt
    21541 tcatggcggt gagacagaat ctcgacgagc tgcggagcgc cgttcgcgcg aagggcctgc
    21601 gcgcaacgcc gtcgcgtctc gccgtcctcg agctgcttcg cgcgtcggat acgccgatgt
    21661 ctcacggcga tgtcgccgat cgcctcgcca ccgaggcgtg ggatcgcgcg acgatctacc
    21721 ggaacctcac cgatctcgcc gaatcggggc tcgtgcgccg taccgacgtt ggcgatcacg
    21781 tatggcggtt cgaggccgta tccgacgatc acgaagcgag cgcgcatccc catttcgtgt
    21841 gcaccgagtg cggcacgatc gcgtgcctgc ccgagatcga gcttgcggtt cgcgcgtcac
    21901 gggcgagccg cggcaaggca ccacgcgcgg tcaagcagcg tcaggtcgaa gtgcacgtgc
    21961 gcggcctctg cgactcctgt cagtagtcac ggcacgacgc gcacgcatgc ttcgtaccac
    22021 gcgagcccga gtcgctcgtc gcgttgcacg aaccagaacg agacgtcggt cggccctttc
    22081 agatccttcg cagccggcgc ggtccagtcg gtgaatagcg gcgctgggtt gccgaacggg
    22141 tcgcgcggtc cgccggtcga gccggccgag aagccaccgg ccgacgcgag gaactggtac
    22201 gtcgggctct cggtgaacat gcgcgacatg ccgtcgatcg tcggcacgac gtactcctcg
    22261 cgcacgccat cgggctcgat cggcatgaac cggatcttct gtcttggcgc gacgaccggc
    22321 ggctcgatac ccgcattgtc gagatcctcg caccgcttca gtgtgagcgg ctggaactcc
    22381 tgatcttcgg cgagccgaac gtcgaccccc gcgagtgacg gattgttgtt tgcctgcagc
    22441 tcgggcggga tccgcggctg cacgcgcaac gacttggccg cgtacagatc gagatccggg
    22501 tcgccatctt cgccgccgac gaccaacgac acgccgtaat agacgccgcc gaggcccttg
    22561 aactgatccg aatcgagcga gtcgaggatc acgccgagga gattgccgtc gaccggcacg
    22621 gtcgcgcaga acgtcggcgg cgcgccggcc atgtctttgt cgtccccttc cggatcgtcc
    22681 cacaggccgg ctccgatcac ggtcttcggt ccgccgtctg ggcagcgctc gtcgctgttg
    22741 agattgcaca gcgtcatcga ccagcggatg cggcgctcga agttgcgatc cgacagcagc
    22801 gcgcagacgt cggtcggtac gagctggtcg agcaggttcg gtggcggcgc cggattcgtg
    22861 atgtcgatgt cgacgacttg ctcgggcacg ctcgcgctca tcgcgagcac gcggaaatcg
    22921 atcacgatgt ccggatcctc gaaggagccg cagccgacga gggccagcgc tatgaccgca
    22981 tgcttcacca tgtgccgcgc actccgatgg tggggaagat cgggaagctt gtcaccggtg
    23041 tcgactcgcg gaagcggtag tcgtagtcga tgccttcctg attcttgcga ttcgtgacgt
    23101 tgatgacgtc gatatacgcg ccgatgctcc acagctggaa caaccacgtc ttctcgatgc
    23161 gcacgtcgag ctgcatgaac gtgtcgacgc gatgcgtgcg catgcccgtc gtcaccgggt
    23221 cgtagcagcc gcagtcggcg tcgaacgtcg cgccgatcac cttcgtatcg ggccggccgc
    23281 tcgcgagctg gaagcgcgca ccgagctcga agccagcacc cggcttgtag ctcgcgacgg
    23341 cattcatcac gtgcggctga tcgaagctcg tcgcgaacca gccctcgccc tcgcgatttg
    23401 tctgtcgcga gcggctgtac gtatacgaga gccagccgaa cgccttctcg ctgatctcgc
    23461 gcttgatgat cgcctcgagg ccgtacgacg cacggcgccc ggtgttgcgg aagttgacgt
    23521 agctgaacgt gccgtccgag ttcatgatga cgtcgggcgt gaacacgatg acgtcccggc
    23581 gatcgacgta atagatctcg ctgtcgaccg accacagccg ctccggcttc cactcgtagc
    23641 cgaggccgta gtgatagccg tgctcgagct ggatgtctgg attgccgaag cgcgtgtcga
    23701 gtgcttctgg ctgcggtggc tggctgaacc gaccgacgta cgccttgccg acgaacttcg
    23761 acgacagctt gtacttggcg acgagccgcg ggtcgaccga ttggcgcgtg ttgccatcga
    23821 gcatgtaacc gtcgaggcgc aggctcggga tcagcttgag gttcgccgtc gcgtcgatgc
    23881 cgaggtcgac atagccgccg agcccgaggc tctgcgagcc gcggaacagc ttcgatggcg
    23941 ggatgtcgac accctgggtc tcgatcaacg tgtcgtcgac cggcacgagt gcctcgaacg
    24001 tggtcacgcg cgacatcagg tcgagcccgg tgtcgagcgt cagcgtcggt cgcagcttgc
    24061 cgttcacgcg catgcgatac gacagcgcct tgttcttcac cgacaggctc gtgaacgggc
    24121 cggacgtctc ggcttgcgca cccgcgaacg tgatcgagtc ggcgccccac gcgggcgaca
    24181 gcgtcagctt gaggtcgcca ccgagcgggc gcgtgtacgt gccgatcacg cggaagaagc
    24241 tcaccgcggt gttcaggttc gccgacgtgt tcgcgtccgg atccttctgg aacacgtgca
    24301 gcgtgtccga cgagccgatc gcgaacagac tcagtcgccc ctcgcgatgg agatcccagt
    24361 cgacgcggcc ctgatagtcg taatagaccg gcgtcacgat ccgctgcgag cctgcggtcg
    24421 gttcgggcag gaacagagga aggatgagat cgatatacga gcgacgtccg gcgaccgcgg
    24481 ccgatacgtc cttcgtgatc ggcgcgcgga tgtagccgcc gctgtcgatc agatcgacct
    24541 tcgcagcgcc gtggatgccg tcgctcttcg tcggccgcga ctcgagtgcg acgacaccgc
    24601 cgtgatgacg gccgaaccgg cccggaaagc ctcccggata cagatcgatc gactcgagca
    24661 tgtcggcgtt gaagatcgac gggccgccga ggaagtggaa caggctgggc acctcgtggc
    24721 cgtcgaggaa gatgccggtg tcgtcgggat tactgccgcg cacgagcaac aggccgaggc
    24781 cgaacggtgg gcgttgcaga ccgggcagcg tctggatcac gcgcaccgga tcgccgaacg
    24841 tgccgggcac gctcgtgaac tgctggcggt gcagcgtgcg cttcgtgacc tcgagctgct
    24901 cgcgctcgcc ctcgacgatc gtctcgtacg gattgccgcc gcgcggccgc atccacagcc
    24961 gcacttcgag tgcctcgcgc ttctcgatga cgacgggccg ctcgaaccga tcgtatttcg
    25021 gatcgaccgc gaggacctgg taatgcccgg gtgcgacgcc gtggaaatag aacgtgccgt
    25081 cgtcgccagt gaccgcatcg aggccgagct cgacgatcga cacgatcaca ccggcgagct
    25141 tcttgcgcgt gccgcgttcg agcgccacgc cctgcagcgt gatcggatcc gacatgatgc
    25201 cggcgtggtt cggcggtccg acttgctggt ctggcttcgg cggcggtggc ggcggttctt
    25261 cctcgacctt cagatcgatc gtgaagccga tcgcggtctc gaccgcgacc tccgccggtt
    25321 tgccgtcgat ctcggccggc tcgaacacgt actgcatcgc ggcggcgacc gcggcttcat
    25381 cgaagccatc gccgacgggc tcgagcacgt cgaccttcgt caccacgccg gtcgcgtcga
    25441 tatggatccg gaccttgacc ttcgcggtct tgccggcggc gagcgccgca ggcggatact
    25501 ccggcgcgac ggcctggatc agctttggcg acttcgtgat cttgccggcc ggtggcgccg
    25561 cgcttcccga gccgctgccc tgcgcatgcg cgatcgcgca caacgcaacc agcgcgacaa
    25621 cgacgcggaa cataggcgcg aagtattgct caggtcgttg tcatctgccg cagatgtgcg
    25681 agcagcttgt cgaacatgcc cttcatggcg ccttcaaggg cgtccttgcg catgctcggc
    25741 aagaacttcg gcttgtactt cgcgaccgga acctggacct tcgcgccgcc cgacaagaac
    25801 gacagcatct tgcccttgtc gtcggtgatc gcgagccgga gctcggcgtc gaccaccata
    25861 taggtgccgt cctgcacgac gtcgagcttg acgaccgaca cgtcgatcat gcgcgggtcg
    25921 aggccccagc gctgggcatc ggctgcgacc attgtcactt gcggcgccgc cttcagctga
    25981 tcctgcagcg cgagcttgag gatgtcagca tgcaatttgc gggtgttctt gtcggtcttg
    26041 cccggcgagt cgtcgttcgt gcccttgatc gtgacgtaga gatcgggttg atcagcgacc
    26101 gcgtgcggtg accgaccgaa gccggtcgtt ttcttacgcg cttgcgccga cccggcggcc
    26161 ggcggcgtga gctggtcgtc cttgatgttg ttcgccttcg cgaccagcac cgcagcttcg
    26221 tgggccttcg cgcgcacggt atcgtcgaga tcatccgttg ccgcattctt gagcgcgggc
    26281 agcgccgcct tgtggccgag cttgccgagc gcggtcgccg cgatcgcgcg cacttgcgcg
    26341 ttgggatcct gaagtgcggt gacaagcggc ttcatcgtgc gcttgtcgcc gaggcgtgcg
    26401 agcgagagca ccgccgacat gcgcgtcttc tcgctcgagc tcgacagcat ttgcgtcagc
    26461 tcggtgatgc gatcaccggc aaacgcaggg gtgcgattca tgacgaccgc tactgcgatc
    26521 atcacgacga gcgcgcatag tcgatttcct cctcgcatgc tcgtgcctcg attagacggg
    26581 ccactcctca acgaagtcaa tgttttctgc gtaacctcgg gtgcgcttcg cgcgcatcgc
    26641 aggtgtatgg tgaagtgatg tctgagcagc cgtaccgcat tggcgtactc gcaggagcac
    26701 tcgtgctggt ggctgtgatc acgtatttgc ggttttgcgg gagtgtcggg ttgcccccga
    26761 agccgccgcc accgacgggg ccgagcggta cgcagtccga gttgctgtcg aagtcgacga
    26821 cctcgccgac catctacaag ggatttctcg atagtgatgc ggccgctgcc ggcgtgcgcg
    26881 ctccgacgat cgatgagatg tcgcgcaagc tgtcgtatcg cgtggacgaa gcgcgccacg
    26941 tgctcgaacc cggtcagcct ccgctcgaag ttgccggttt gcggattcga ctcgagcgca
    27001 cgagcgatca agtcgtgatg gtgatccaga acatgctcgg ttccgacgct gcgtacgaag
    27061 tgacgtcgtc gccgagtacg ggctccgcgc tatgcaaggg tgcgcgtgcg ctgccgttca
    27121 atgcgatggt gatctcgaag gggcagagcg aacgccgcac cgagtgcacg tggcgcgatg
    27181 gcatgtcgat catcgtgacg aaggtcgaaa cgatcgagct ggcgccgcta ccggcttggt
    27241 acatcagcca agttccaccg caggtcgtgg gcattgaaga ccgactcgcg cgggggcatc
    27301 gtggggtcga cacgaaggaa aaatgctccg cggttcagtc ccaagtggtc cgaactgggc
    27361 tcgatcgggg cgatattggg tggcgagatc tgatcgactt ctactcccgg caccgctgcc
    27421 agacctacca gtttccgtcg acttatcgcg catttaagtc tgacggcgag cggcgcgtcc
    27481 cagccggtgg ctgagtgtcg gacgagtttc cgcggagata cagttgcgct cggcccaagt
    27541 ccccgtttag cctaaggtct aggtaacgcg gagtcacggg tgaccaagtc cgagctcatc
    27601 gagaagatct ccgagacgtt gaagctgccg gctggtaagg cggaggccat cgtcaacacc
    27661 atctttgatt ccatggtgaa ggcactagag cgcggcgaag gcatcgagat tcgcggcttt
    27721 ggtagcttca ccgtgcgcaa gtacaaagcc tacgaaggta ggaacccgcg caccggcgag
    27781 acagttcatg tcgccgagaa gcggcttcct ttcttcaagg tcggcaaaga cttgcgtgaa
    27841 cgcgtgaacg caggtctcgg gacgccgctt ccggcggata aacccgacga gcctgacgac
    27901 accgatccga acgagcccga gtacgacgac gacgaggact agttcctcgt cggtaagtag
    27961 gcgtcgtttg ggcatttcct cgtcgaggaa atggtttcgt gcgtctgatc gtcgtcggta
    28021 agtgagcgtt ttctcgtcga ggaagtgcgt cgctcacgcg atcgcgcatc gtagatcagc
    28081 gttttctcga cgaggaatcg taagtcagcg acatcgcgat gatcgcgtcg gcgctgctcg
    28141 agccgctgac gatcctcgcg tcgagtgatc gcgatgttgc tcgattcgct ggatcgcggt
    28201 gttgctcgat ccgctggatc gcggtgtcga gtgatcgcgc ggtgttgctc gatcctagcg
    28261 atcggaggac ggcgtgaccg tgccgccatc gccatcgacc gtcacgatca ttccatcgct
    28321 gagcttcgtc catgcgtcgc gacaaccgac gacgcacggg atcccgagct cgcgcgcgag
    28381 tgcggcgcca tgatcgagca agcctccggt ttcgctgacg agtgccgcac aaccggcgac
    28441 gagcacggca agcgctggcg taactgcgcg agttacgacg acgtcgccgg aaccgaccgc
    28501 gatcgcgctc gccagtgagg cgaaccgcac gacgcggccg gcgacgcgcg cgccagtacc
    28561 gacaccacgc aatgacggac ccggtttggc ggcatcgcca ccaaccacga tcggcatctc
    28621 ccactgtgca gcgcgttcag cggccgcgcg ggcgcccgcg gcgcggcgat gggcgtcatc
    28681 tggatcgaac gctgtcgccg cgatggtctc gtcgagcggg agccagaaga tgtcgtcacc
    28741 acgcaacttc agctcgctgc tgcgggccaa cagcgcgcga cgtacgatcc attgcgcacg
    28801 cgcgaacagc atgtcgtcgc gctcggccag atccgcggcg actcgcgcga tgtcgacctc
    28861 gaccgcgtgc tcggcaaaca gagccggatt cggaaccgcc ggttgttcgc cggtcttgcg
    28921 gtcgtcgcga gcccgctcct gctgcgtgag cttggcgcgc gcgatcgcat cccgcagtaa
    28981 tcccggtcgc tcagcatacg ttggcgcgac cacgtcccac gcaggagcga gcacgccgag
    29041 tcgggcttcg acaacatcgt catcgaggtc gccgcgcgcg gctgcccgca gtgttgcctc
    29101 gacggtcgat gccggccgct cgaccagcga cgccgcgatc gcatccgggt gggccgagcc
    29161 atgggcgcgt aacttcgcgg gcaacatcgc gcgcgccgcg ctgatcagcg gcgacagctc
    29221 gcgcgcccag atcgcgtaga acgcgaggta gcgctcgacc gcttcggcga gtggcagtgg
    29281 cttgccgccg tcgacgagcg tccgctcgag tcgcgcttgc agctcggtcg catgcgcgat
    29341 cagctcagcc gcgtcgcgca cgatcggtag ctcctcgccg cgcggagccg agtacaagaa
    29401 cccggcgcag atgcgcagcg accatggtgc gatgcctgcg cgctcgacgc gctcgacgag
    29461 gccctgctgc gcgaccgata gcggatcggg gttgtgtgcg acatcccagg tccacgtgcg
    29521 gccgtcggca agtggtgcga tcacggcggg cggcggcgcc gcaagtgtgc gcgtcgtcgg
    29581 atgcacgatc ggccgtgcct gcacgatcat cgtctcgatc gcgacgtcga acccactctg
    29641 cttgcggagc tgtaccagct cgacgtcgac gccgtgcggc tgatcgagcg cggtctcggc
    29701 gcgcagggcg agcaccgccg catgctgatg ttcgatctcg cgcggtaggt catcacgcga
    29761 gtgccgggtg acgtgatcgt gatgctggat caagatttcg cgcgacgccg gctgaccggg
    29821 cggccgcgtg tagacgacga gcggcgtccc cgatacgtac tcctgcacga tcacgccgat
    29881 cgcaatcgtg ccgccacgcc gcagcgcata ggccgcggcc agcggagtaa gcgcgctctg
    29941 ccagaccgcg cgaatcgcat cccacacttc gtcgaccggc accgcggtgc gcgacgaaaa
    30001 cacaccggcc gccgagcccg ctgcgccgtc ctcgatcgtc gccgacgacc gcaccatcac
    30061 gacgtggccg agctcgtgtg cgcgcgagcc gaccgcatcg agtagctcgg gcggcagcct
    30121 cgcgatcgcg attcgttgcg cggcttcctc gagcacgtgg ccgatgtgag cggtgtcggt
    30181 aatcgcgagc tcgccgacga cgctgcgaaa tgcgcggtcg tcgatcacga acccgggagg
    30241 gactggcaac ccggccgcga gaagccggcc cagccccaca gctttcccgc cgcagctcgc
    30301 gtccgcgtcc cgcaggtcgt gcacccacat gcaaagcaca gtacgatcgt ttcgtgcgtc
    30361 tggctgtgat tgtctcgttg ttcgcgctaa ctgcggcaac ggcacgcgcc gacgacagca
    30421 agctcgcgaa agaagacgtc ccgctccagg cgaaggcgac tctgcgcggc aagaacaagc
    30481 tgtgctgcgg ctacccgctc gtcgacgaca tgcagtgggc gctcaggttc tactggatgg
    30541 tcctcgaatc cgattacgac gagccggcca gcccgaacgt gccgcgtagc ggccgcggca
    30601 aggagcaggt cagcgagacc gcgcgcgtcg acgtgtacac gccactcgga ttcttcctcg
    30661 gccgcatgtc cgagaagttt gcctgggcgc tgcgcctcga aggctcgggc ctcatggcgg
    30721 acggtcgcgt catcaactat gccggcaagt gcgacgttgg ctatgcgacg aaggtggcgg
    30781 gccaaccgat caaggcgacc tgcttcgagc agctcgacgt cgagcagttt ccgttcgggc
    30841 gtggcgccgg gttccggccg ctcattccgt tcaagtcggt cgcgatcgat ccgcgcctcg
    30901 tgccgctcgg cgagccgctc tacattccgg agttcgacgg catgatgctc ccggacggct
    30961 cgatccacga cggctgcgtt cgcgccgacg acacgggcgg cggcatcaag aagcgcaaga
    31021 tggacttctt cgtcgtcacc tacggcaact tccgcttctt gctcgaccag ctacagggcg
    31081 tcacgtggat cacgccgcac gtcgaggcgc cgcgctgcga gtacctccgc gaacgctaaa
    31141 ggtgcgagac gatgccgctc gtcaccacga cgcggccggc gtgacgctgc tcgatttcgg
    31201 agaggcccgc acacagcgcg cgacgacttg cttccggtgc atggccacga tcgacgaaca
    31261 ccagtacgcc gccgtcgcgg accacacgcg tccaatcctt gagcgtgatc tcccagccat
    31321 catccgtggc gatatcaacg ccaatgaccg ccgctagcga tcgatcttcg atcgatgaca
    31381 gatcggccaa cgcattcgtc agctttttcg ccgcgcgctc ggacagtccg atcggcacga
    31441 catttcgctt cgcgtcgcgt aacgcggtga cgagcttcgc gttgccgatc accgcgagct
    31501 tgccatcagc atgcgcggtc gcgagcgctt cgccgatcgc agtcaccgcg ggggtcacga
    31561 acaggccatc caacacgggc cactcacata ccacgaacgg acccagagac tgcgatcatc
    31621 cgaatgggcc cgacgtccga tgccctactg aggccggagg tcagaggcct ctggccctgc
    31681 tacaccggtg gccggtcgtc cgcgccggtg tcggcgtctc gcttcggcga gtgccagatc
    31741 agatccatct gcggaccctt ttcgcccttg gagtgatcgc cgcgatcacc cggcttcaca
    31801 ccgggcggcg tctcccacgg gatcttgggg ttcggatagc ccttcgctgc ctcgtacatg
    31861 taccgcgccc acagcgggac caccgtcatg tacgcggcgt ctttcttgcc gagtgcacgc
    31921 tgcttcttgt cgtcgcccat ccacaccacc gtcgtgaact tcgacgtgta gccgatgaac
    31981 cagttgtcgt gcgtcgcgct cgacgtgccg gtcttgccgg cggcgatcag cccggtcgcg
    32041 cgaaggatgt tcgcgaagcc aaactcgact tccgacgcga gcagcttcga cgtcaagaac
    32101 gccgatcgcg ccggaatggc ctccttcggc tggatgccgg caagcacggc gaaccgatcg
    32161 aaccgatcgc cgaggccgag ctgcggatcc tcgatcgccg tgttgtcttc gatcgtgttg
    32221 ccgtcgcgat cgagaatgcg ccgcacgtag atccaatcct tctcttggcc gaccgggcgc
    32281 ggccaccact tgccgttgcg cgcaaagatc gtgaacgcac gcgccatctc gtcgaccttc
    32341 gtgcacgacg cgccgagcgc gagcgcatcg tcggggatga tcttcgtcgt gaagccgagc
    32401 cggcgcgccc atttctcgac gttcttcgcg cccagccgct tgaacagatc gatcgagggc
    32461 acgttcttcg agaacacgag cgcgtactcg agcgagacgt tgccgtcctg cgtgtcgccg
    32521 aggttcgtcg gcgtccagat cgcgccggtg tcgggatcga cgatctggac cggaatatcg
    32581 ttgagcaccg tgtcgaagcc gtagccctcg tcgagaccga gtgcgtagta gatcggcttg
    32641 tacgtcgacc ctggctgccg gcacgcctgc acggcgcggt tgaacaccga gcggtcgtag
    32701 tcgtaaccgc ccaccatcgc gacgacgtag ccggtgcggt gatcacccgt gaagatcgtc
    32761 gtctgcggat gcggtacctg ctcgagccgc acgacgtcgg tgtgggcctc gtcccagtcc
    32821 ttctggttgt ccgagtacac ccacgacggg ttgatcttgt cgggcaggcc gtactcgcga
    32881 aacttctcga tcgtcttcgt ctcgcgcgac acccacacga cgtagccgga cttgagcgcc
    32941 tcgcgcgggc tctcgatcgt cttgtcgttc tcggcgttgc cggcttccca cttcgccgcc
    33001 cagctcatgc cgcgcatcgg cagcgtgacg cgcttgtcgc cgatcagcag ctcggccttg
    33061 tcgtcgccaa ctttgtcgac gagcgcgagg tagcgcttgc cacgcacgag cgggccgttg
    33121 ccgtagagct gcttctggcg ctcgaggaac gtcgcgcgtg cggcgccgtc gatgcgccac
    33181 tcggggccgc gccagccttg ccgcttgtcc tggttgtgcg cgccgtagtc ggcgttcgcg
    33241 tacgcggcgg cttcccaggt cggctcggcg gcggtctcga cgcgcagccc ttcgctctgc
    33301 agcgcgtagc gttcggtcat gtagcgacga atctgctcgg cgtagtacgg catgcgatcc
    33361 ggaaacacgt cgcgatacat gtcgagcttg atcggcgttg ctttggcctt gtcgacgtca
    33421 gcgcgcgacg cgaagccgta cagcgccatc ttctcgagca cgatattgcg gcggtcggtc
    33481 gcgactttcg ggcggtgcac gggcgagtac gcggttggcg ccttcgcgag gcccgcgatc
    33541 atcgcgcact cgtcgagcgt cagctgatcg aggtctttct ggaagtagcg gcgcgcggcg
    33601 gcggcgacgc cccaggcgcc tgcgcccaga tagatgtgat tgaggtagac cgcgaggatc
    33661 gcgcgcttgg agtacgtcgc ttcgaggcgg cgggcgagga tcgcttcctt cgctttgcgc
    33721 gagagcgatt tctcggagcc gaggaactgc ttggccacct gctgcgtgat cgtcgagccg
    33781 ccctgcgcga agtcgccggc ggtgatgttc gtccacaccg cgcgggcgat gcccttgaag
    33841 tagaggccgc ggtggttgaa gtagtcgtga tcctcgaccg cgaggaacgc gtcgacgagc
    33901 tgctttggaa tcgcctcgta cggaacgacc tcgcgccact cctgcgcgaa ctcgccgagc
    33961 agggtgccgt cggcggcgta catgcgcgac acggccggca ccgtcttcga gtacgcggcc
    34021 aggtcgtgcc gcgtgcccgg gccgacgtca tccgtccgga ccgagaaata gctgtagagg
    34081 atgaagccga acagcgcggt cgctgtcaca ccgaccagcg cggcaaatcc gtagagcttg
    34141 ccgatccagt acgcgatgcc accgtctgcc cggttcgcga tcgctactcg gctgcggtcg
    34201 tgaatcggag acacagcagg ctcagacaca gccgatggat tctagccatc ccacgcccga
    34261 aagcagcaag tgctaggctc tgtctgatgc gccttctgtg ggtattcgtc gtcggaatcg
    34321 cggcatttgg ctgcaaggac aagcagcccg cgaaatctgc cgacccggca cccaaaggaa
    34381 ccacacccac aactcccgga tctcccacga gtgcggcggt gtctcagccc agatccgcag
    34441 cgccaacgct tccagccgac gaattattcg ccaaagagag caccgattcg gagtgggcgc
    34501 ctggtgccga gcgtgcggtc cgcgcggtcg cgcccgagct cgaggacctc gcgtgcaagc
    34561 acgatcagtg tcgggccacg gttgtcgcga ccaacgagac cgagctcatg gccgcggtcg
    34621 acaagctcca gacgcccgag tcattgctcg gtgtgccgga agcgaagtcg atcaagctca
    34681 cgcagccggt gcaaagcggc aacggctaca cgatgacgat ctacatccgc tttgatcgcg
    34741 aataactcgg cgctacgcga gcgcgaggtc tgcgatcgcg ctgtcaccgc tgatctgcgt
    34801 caggcaccgg acgaggtgcg cgcggatctt gtgcttcttc gagtagaccg tcttgaggct
    34861 gatctgcatc tcggcggcga tctcgtcggc ctcgaggccc ttctggtagt agagctcgac
    34921 gaacgtgcgg tccttgtccg tgaactcggc gagcaaaccg ttcaggtgat cccagcgctc
    34981 cttctcgatc agctgatcga gcggcgtgcg atcgcactct tcgtgcggat cgagcgcgcc
    35041 atcgaccttg tcgagcagcg ggcgacggcc ggcacagcgc aagaagtcgt aggccgcgtt
    35101 gaccgagatc atgccgatcc acgaaccgag cttcgtgccg cgcgccgggt tgtagatgcg
    35161 gagcttgtgc atgtcgtcgc ggacgagctg catcatgacg tcggcgtaaa tctcatcgag
    35221 gtccgcgttg cccagcgtgg gggcgtactt cagcgtgacc ttggtgatgc agcgatagat
    35281 gagcgggcga taacggcgaa ccagctccga ccagccacgg gcgtcgctac gcagaaccca
    35341 cttgagcaac tcgcggtcgg tccactccgt tgggtttttg ctggtagcgc gggcgagaag
    35401 agccattggt tcctcctggt gggtcgaccc ttagagcaac gccggtgcca cgcctaaccg
    35461 actgaatttt caggctctgc tccggcaccc acaggtggcc tccaccaaac attggtgaaa
    35521 tcgaccagcg ggaggaagca agccgtgatc ggcgacgtac taatcactcc cgccgcgacg
    35581 aatgtgcctt agttccgtgt ccggtcaccg gccacacgca tcggtggtcg taccatccgc
    35641 gccatgttcg gaccgcacga tggccggcgt gtagacgttc acccgtaccg ccggatgatg
    35701 acgtttctga tccgcgggcg aaacgagtcc gcggtctatt tcgagcagcg gctcgatgtg
    35761 tcgcgcgcgc aagcgtggct cgatcaggcg aaccaccgcg ccgtaagccc tccgtcgaga
    35821 acacggagtc gaacattgca cccgctgtcg caggccaatc gccgaacgcg cagcccgcga
    35881 gcgccgcttc ggcggttggc aggtgctctg cctcgattgt gctctcgacg atccgccggc
    35941 gaccgactag cgctacgcct tcgcgacggc ggcgtcccag cgggtgaggt gttccttcac
    36001 ccacgcgcga tcggttgtcg gcttgaaccg gcgttgctcg cgccaggtcg ctttcacggt
    36061 gtcgaggtcc ttccacacgc cggtgccgag gccggcgagg aacgctgcgc cgagcgccgt
    36121 cgactcgacg agctcgggcc gtgcgatctc cacgccgagc acgtcggact ggaactgcat
    36181 gagcagatcg ttcgccgccg caccgccatc gaccttgagc atcgtcagcg ggcgacccgc
    36241 gtcgcgctcc atcgcgcgca agatgtcgac gttctgcagt gcgatgccct cgagcgtcgc
    36301 gcgagcgaga tgcgccttcg tcgtgccgcg ggtgatgccg gtgatcgcgg cgcgcgcgtc
    36361 gggacgccag tgcggcgcac cgagacccgc gaaggccggc acgacgatca cgccgcccga
    36421 ttcgggtacc gagcgcgcga gctcttcgat ctccgatgcg ctcttgatga tgccgagccc
    36481 atcgcgcagc cactgcaccg cggcgccggc gatgaaggcc gagccttcga gcgcgtagcg
    36541 cagctcgccc gtgtgcagct tccacgcgac ggtggtgagc aggcccgact tcgactgcac
    36601 cgcccattcg ccggtgttca tcaggatgaa cgcgccagtt ccgtaggtgc atttggcgtc
    36661 gcccggcgcg aagcaggtct ggccgaacag cgccgattgc tgatcacccg cgaggcccgc
    36721 gatcggcacg ccatcgcgaa tgccgggcac gcccttcgtg gtgccgaccg ggcccgacga
    36781 cgggacgacg ttcggcagca gcgcgcgcgg cacgcccatc agctcgagga gctcgtcggt
    36841 aaacgtcagc tccttgagat cgaagagcag cgtgcgcgat gcgttgctga cgtcggtcgc
    36901 gtgcacggcg ccgccggtca gccgccacag caagtagcaa tcgatcgtgc cgaacgcgag
    36961 cgagccatcg cgcgccgcag ccgcgagccc gggcacgttg ttcaacatcc agtggacttt
    37021 ggtgcccgag aagtacggat cgagcgtgag cccggtgagc tgcttgacgc gcgcttcctt
    37081 gccctgcgcc ttgagctcgg cgcagcgatc ggcggtgcgg cggtcctgcc acacgatcgc
    37141 gttgtgggcc gctttgccgg tcttgcgatc ccagatcacc gcggtctcgc gctgattcgt
    37201 gatgccgatc gcggcgacgc tcgacggctc gatgccctgc atcgcggctg cgagtgcggt
    37261 cgtcaccgac gtccagatgt cttccggatc gtgctcgacc caacctggct tcggatagat
    37321 ctggcgaaac tccttgtagc cgcggccgcg gacttggagt tgctcgtcga gaacgagcac
    37381 ggtggaaccg gtggtgccct ggtcgatcgc gatcacgtgg cgcatgcaag atctccgctc
    37441 tgttcgtggg gccggtctgt tgtcctgtca gttgtccgca cattgcggcg caagaagtcg
    37501 atgacttcag cggcaacgat gtcgcgttcg acatcggccg cgatgaggtg atagctgcgc
    37561 ggcaggatgc gcatgcgcgc ggcgcacgtc agctgtgcga tccgcgtcgc gcacgcgacc
    37621 ggcgcggtgt ggtcgtgctc gccgtgcagc acgagcaccg gctgcttgat cgacgacagc
    37681 tcgccctcga cgacgcgcat gaacgcggcg agctcggcga gcgcgcgcgt cggaatcgcg
    37741 ctgtagcagg ggttctcggc tttcgtgcgc ttgtcacgca cgtcggagcc atagaatttc
    37801 ggaatcgcgc ggatgcgccg aagcatcccg tctgccgccc attgcgcgac acgcgccgac
    37861 agtccttcga gccacagcgg tgccgcgagc gacgccacgg ctgcaacatc ggtgcgcttg
    37921 cttgcgagat gcagcgcgag caggccaccg agcgattgtc caactaccgc gacttggttg
    37981 cacaacagcc gcatcgagtc gtaggcgtcc tcgacggcgt cggcccaatc cgaccacttc
    38041 gtttgatcga gatcctcgac gcgcgtgcca tggccgggca ggcgcagtcc atgcacggtg
    38101 aaacctgcgc gcgccaattg gtcgccgaga taacgcatct cgtaaggcgt tccggtgaag
    38161 ccatgcacgc acaccacgcc gatcgcgcca tcaccgacga gatcgaaagg ctccccgagc
    38221 gccacgtcgc ttcgttatca caccggtgcg cacattgaca gcgcacccca gtccgcgctc
    38281 tgatcgaaag gatcacggcg ggttgcgtgc tcccgtgcga tcggcgttcg gatccggcga
    38341 tgagcgagtg gaagacacgg attaccaaag tccaggtcgt caagccggcc aaggaaagcg
    38401 gcgaggcgtg tctcgttctc atttatccgc ccggtccgga tatgggaaag cgttttgcgc
    38461 tgacgcgaaa cgaaatcgtg ctcggccgcg gcgcggattg tgacatccag gtcgatcgcg
    38521 attcagtatc gcgccgtcac gcacgcgtct atcgcacggg cgagtcgtgg tccgtcgagg
    38581 atctgcagtc gacgaacggc agctacgtca acgacgtgcc ggtcacgaag tccgtgctgc
    38641 gcgacgccga cttcgtcaag atcggcgctg cgatcttcaa gttcctctcg ggcagcggca
    38701 tcgaggccag ctaccacgaa gagatttaca agatgacgat cgtggacgcc ctcaccggtg
    38761 cccacaacaa gcgctacttt ctcgaattcc tcgagcgcga gattgctcgc tgcgcgcgct
    38821 atcgccgccc gctctcgctc ttgatgttcg acatcgacca cttcaagcag atcaacgaca
    38881 agcacggtca cctcacgggc gactacgtgc tgcgcgagat gtcgcgccgt ctgctcgggc
    38941 gcgtgcgtcg cgaagaattg ctcgcgcgct acggcggtga agaattcgcg gcagtgctgc
    39001 ccgagacgga tctcaccggc gcgcgcacgt tcggcgagca gattcgccga ctcgtcgcgg
    39061 atcagccgtt cgaatacgaa ggcgatacgt ttccggtcac cgtgtcggtc ggcgtcgcga
    39121 ccgtcgaggg tgaggacatc gacgtccagg cgttcatcaa gaccgccgac gatcacctct
    39181 accgagcgaa gcgcgaaggt cggaatcgcg tcatcggctg atcgccgatg gtgggcgtca
    39241 tcggttaagg cggcggcgcg tcgggcgcgt tgcggaagtc cgtcacgccc gcttggccga
    39301 tccagtactc gatgtcgacg ctgttatcgg cgttgatcgt gatgaagcgc agcgcgccgt
    39361 agatggagcc ttgcttgaag acgagcacac ccttcgagca cgtcgaccct tcggtaccga
    39421 acacgaccga accgtccgtg ctgtcgaacg tgtagctcgt cgcgtcggtg agatcgtgaa
    39481 tcgtcgtgac gccggtgtag ccgcggccgg tgatgccgc
//
LOCUS       DASUFX010000043         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_701059,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000043 DASUFX010000000
VERSION     DASUFX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701059"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFV99_01710"
     CDS             complement(<1..605)
                     /locus_tag="VFV99_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5058039.1"
                     /translation="MLLRLLRLYLRPYGKPMAIIVVLQLAATIASLYLPSINGDIVER
                     GVVTGDANYILVHGGIMLAIATVQIVCSAAAVYFGARVAMSYGRDLRAGIFHHVGKLS
                     AREVGKVGAPSLITRTTNDVQQVQMLVMMTATMLVMAPIMCISGIVMAVREDLALSKL
                     LLVCIPALAISIGLIIGRMIPKFRVMQPKIDTLNRVLREQIT"
     gene            complement(605..1186)
                     /locus_tag="VFV99_01715"
     CDS             complement(605..1186)
                     /locus_tag="VFV99_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5058040.1"
                     /translation="MARAAPLSPDARRASIIAATLPLLRVHGREVTTSQIALAAGVAE
                     GTLFRVFPDKDALIEAAIAHAFDPGPAESELGRIDPALSLREKLIQVAEVLQRRFAQI
                     WELMSMLRLAPPILEQRPKAPPPDEVRLRMLLEAVFEPHRDEIRCEPEQAARLLRGLA
                     FAGLHPRMTDKPLTPHEIVSLLLDGVRARDEED"
     gene            1273..1584
                     /locus_tag="VFV99_01720"
     CDS             1273..1584
                     /locus_tag="VFV99_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEX5058041.1"
                     /translation="MGRKRPDRTARRAQERAARQLVRDKEKLAGMVAGGSAERPIEVT
                     SSAVIEGRVRATPCPQCEGELRVKDHRSTGSGMRAVDVRCDQCGAPRTLWFRIVSDEP
                     N"
     gene            1602..1862
                     /locus_tag="VFV99_01725"
     CDS             1602..1862
                     /locus_tag="VFV99_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058042.1"
                     /translation="MECRSGCGACCIAPSISPSYPALPEGKPAGERCPHLTDDKRCAL
                     FGKPERPSACVGLRPGPEMCGESFEQAMSTLLRWEDLTTPRR"
     gene            1871..2134
                     /locus_tag="VFV99_01730"
     CDS             1871..2134
                     /locus_tag="VFV99_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058043.1"
                     /translation="MSSGDGPVARCKLCERTAVGPCARCRAPVCGDCCELTDGGATTF
                     AICLSCVKRGGSSLAPAWLGLLGWLAVIIVALGGIGLLLAFLR"
     gene            2264..2995
                     /locus_tag="VFV99_01735"
     CDS             2264..2995
                     /locus_tag="VFV99_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5058044.1"
                     /translation="MRLLPLLLVTALVAVVGAGCQGQMFARGTFVAEAPTPVIRADVN
                     VQISFFGIPLAGAQDVVFVLDRSGSMRGLAAGFAGDDVGMNKAASTIVGIGTQLAGSG
                     HHSKMEAAKNELVHTLQMLPDGTRFMIIFFDDELAAFAPTMLTLDPNTRTAAINFVRG
                     IKPGGSTAAVPALRLAYKAGAARVILLSDGLANTGGGANDLLAEARERMQYGVRFDTV
                     GLGIDQDSALLNTLSAESGGLAIKR"
     gene            3010..3618
                     /locus_tag="VFV99_01740"
     CDS             3010..3618
                     /locus_tag="VFV99_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058045.1"
                     /translation="MRALLFVPLVACAQPHAAKPVPRPEPASPIADVMEPFDMNGYVL
                     AGAEYWPYIGKEDIRYPQDVLWGFYPEKGVVAPGESDPNADTASRDAVDCAQQSFAAL
                     QVFLAKNPPALRRIVELGEAKGFVPRFYLWTNDYTHAATPYPPGVREARLWYWKRKQP
                     EPPKPPGYWKWEATVTQNGICEIPKQEQIDEYLAETLKTLER"
BASE COUNT          661 a         1213 c         1302 g          666 t
ORIGIN      
        1 gtgatctgct cgcgcagcac gcggttcagc gtgtcgatct tcggctgcat cacgcggaac
       61 tttggaatca tccggccgat gatgaggccg atgctgatcg caagcgccgg gatgcagacg
      121 agcagcagct tggacagcgc gagatcctcg cgcacagcca tcacgatgcc gcttatgcac
      181 atgatcggcg ccatcacgag catcgtcgcc gtcatcatca cgagcatctg gacctgctgc
      241 acgtcgttcg tcgtgcgcgt gatcagtgac ggcgcgccga ccttgccgac ctcgcgtgcg
      301 ctcagcttgc cgacgtgatg gaagatgccg gcgcgtagat cgcggccata gctcatcgcg
      361 acacgcgcgc cgaagtagac cgcggctgcg gagcacacga tctgcaccgt cgcgatcgcg
      421 agcatgatgc cgccgtggac gaggatgtag ttggcgtcgc cggtcacgac accgcgctcg
      481 acgatgtcgc cgttgatact cggcaggtag agcgatgcga tcgtcgctgc aagctgcagc
      541 acgacgatga tcgccatcgg cttgccgtac ggtcgcagat acaagcggag caggcggagc
      601 aacatcagtc ctcctcgtca cgtgcgcgga cgccgtcgag caacaacgag acgatttcgt
      661 gcggcgtaag tggtttgtcg gtcatgcgcg ggtgcaggcc ggcgaatgcg aggccgcgca
      721 gcaagcgcgc tgcttgttcg ggctcgcaac ggatctcgtc gcgatgcggt tcgaacaccg
      781 cctcgagcag catccgcagt cgtacttcat cgggcggcgg cgctttcggg cgctgctcga
      841 gtatcggtgg cgcgagccgg agcatggaca tgagctccca gatctgcgcg aagcggcgct
      901 gcagtacttc ggcaacctgg atcaacttct cgcgcagcga tagcgcggga tcgatacgcc
      961 cgagctcgct ttcggcgggt cctggatcga acgcgtgcgc gatcgccgcc tcgatcagtg
     1021 cgtccttgtc ggggaacacg cggaacagcg tgccctccgc gacaccggcc gcgagcgcga
     1081 tctgcgacgt cgtcacttcg cggccgtgga cgcgcaacag cggcaacgtc gccgcgatga
     1141 tgctcgcgcg ccgagcatcg ggagacagag gtgccgcgcg agccatcgcg tactaggtaa
     1201 ctgagtgagc actcactcgc aagcagatca tgctgatatt agggtagatt tctcgtaacc
     1261 tcgcgaacgg acatgggacg caaaagaccg gatcggacgg cgcggcgggc acaggagcgt
     1321 gccgcgcgtc agctcgtgcg cgacaaggag aagctcgcgg gcatggtcgc cggcggttcc
     1381 gcggagcgac cgatcgaggt gacgtcgtcg gccgtgatcg aaggccgcgt gcgcgccacg
     1441 ccgtgtccgc agtgcgaagg cgagctgcgc gtcaaggatc atcgctcgac cggcagcggt
     1501 atgcgcgccg tcgacgtgcg ctgcgatcaa tgcggagctc cgcggacgct gtggttccgg
     1561 atcgtcagcg acgaaccgaa ctgaaatgct acggtgagcg catggagtgt cgctcgggct
     1621 gcggcgcgtg ttgcatcgcg ccgtcgatct cgccgtcgta tccggcgttg cccgagggaa
     1681 aaccggccgg cgagcgctgc ccgcatctca ccgacgacaa acgctgcgcg ttgttcggca
     1741 agcccgagcg accgagcgca tgcgtcggcc tcaggccggg gccggagatg tgcggcgaat
     1801 cgttcgagca agcgatgtcc acgctgcttc gctgggaaga cctgacgacg ccacgccgat
     1861 gacgtcgttg atgtcgagcg gagatgggcc ggtcgcacgc tgcaagctgt gcgagcgcac
     1921 cgccgttgga ccgtgtgcgc ggtgccgtgc gccggtgtgt ggtgattgct gcgagctgac
     1981 cgatggtggt gcgacgacgt tcgcgatctg cctctcgtgc gtgaagcgcg gcggctcgtc
     2041 gctcgcaccg gcgtggctcg gcctcctcgg ctggctcgcc gtgatcatcg tcgcgctcgg
     2101 cggcatcggg cttttgctgg cattcctgcg gtagccgtca cgcatacacg cggtgtggtg
     2161 ggccgaaacc tggcggcgtg tgaagccgcg ttctcgtcga gcgcgctaag tccgcgccgg
     2221 atggtcgaga cggttggtcc aacggctgca ctaagggtcg accatgcgtt tgcttccctt
     2281 actgctcgtt accgccctcg ttgcggtggt cggtgctggt tgccagggtc agatgttcgc
     2341 gcgcggcacg ttcgtcgccg aagcgccgac ccctgtgatt cgcgccgacg tcaacgtgca
     2401 gatcagcttc ttcgggattc cgctcgcggg tgcgcaggat gtcgtgttcg tgctcgaccg
     2461 ctcgggctcg atgcgcggcc tcgcggcggg cttcgcaggc gacgacgtcg gtatgaacaa
     2521 ggcggcgtcc acgatcgttg gtatcggtac gcagctcgcc ggctcgggtc accactcgaa
     2581 gatggaagcc gcgaagaacg agctcgtgca cacgctgcag atgctgcccg atggcacgcg
     2641 cttcatgatc atcttcttcg atgacgagct cgccgcgttc gcgcccacga tgctcacgct
     2701 cgatccgaat acgcgcactg ccgcgatcaa cttcgtccgc ggcatcaagc cgggtggttc
     2761 gacagcggcg gttcccgcac tgcgccttgc ctacaaagca ggggcagctc gcgtgatctt
     2821 gctgtcagac ggccttgcga acacgggcgg cggcgcgaac gatctgctcg cggaagcgcg
     2881 cgagcgaatg caatacggcg tgcgattcga cacggttggc ctcggtatcg atcaggactc
     2941 ggcgctgctc aacacgctgt cagcggagag cggtggactc gcgatcaagc gctgagctat
     3001 ttagcgcgca tgcgcgctct cctgtttgtt ccgctcgttg cgtgtgcaca gccacacgcc
     3061 gcgaagccgg tgccgagacc agagccggca tcgccgatcg ccgacgtcat ggaaccgttc
     3121 gacatgaacg gctacgtgct cgcgggtgca gagtactggc cgtacatcgg caaggaagac
     3181 attcgctatc cgcaggacgt actctggggc ttctatccgg agaagggtgt ggtcgcaccg
     3241 ggcgagagcg atccgaacgc ggacacggcg tcgcgcgatg ccgtcgattg cgcgcagcaa
     3301 tcgttcgccg cgctgcaggt gttcctcgcg aagaatccgc ccgcgctgcg ccgcatcgtc
     3361 gagctcggtg aagcgaaagg cttcgtgccg cgcttctatc tgtggacgaa cgattacacg
     3421 cacgcggcga cgccgtatcc gcccggcgtg cgcgaagcgc gcctctggta ctggaagcgc
     3481 aagcagcccg agccgccgaa gccacccggc tactggaagt gggaagcgac cgtgacgcag
     3541 aacggaatct gcgagatccc gaagcaagag cagatcgacg agtacctcgc cgaaacgctg
     3601 aaaacgctcg agcgatagcg ccagggactc gactcccacc gctgtgtggg agcctcccag
     3661 gccgacgagc ttgccgacgg caactcgagc agccaccgct gcgcgcagca acgtcgcgaa
     3721 agtgatcaac ttgcgctggg cgatcagttg cagaggttac gcgccatgcg cgtccttgcc
     3781 ttgatggtgc tgctcgctgc ctgcgcgacc gagacaacga tcgattccga tcacgatgag
     3841 cc
//
LOCUS       DASUFX010000044         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1673958, whole genome shotgun sequence.
ACCESSION   DASUFX010000044 DASUFX010000000
VERSION     DASUFX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673958"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VFV99_01745"
     CDS             <1..1693
                     /locus_tag="VFV99_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5058046.1"
                     /translation="QIRVRQTYLVVLLDDRAVQLLPSTGRVSIGRATDAQVVIQHPSV
                     SREHAVLDVEQLTLTDVGSRNGTYVGGQRITPNTPIHLGRGETIVVGEVTMFVQQDGA
                     SAPALAAPIPDAPLELRLVDETARAARHGSPFVHVRVHVPEPQADAAREVLADALRSS
                     DVMSEEAPGRFQLLLPAITSERGRPVIQRLVQTLDRSGIEARTGIACWPQDGVTAAQL
                     SAHARELVAAKARNASPMDEVMRLAASVASTEITVLITGETGVGKELIAEMIHRLSPR
                     SQAPFMRINCAAIAEPLLESELFGHAKGSFTGADSARIGLFEAGAGGTVLLDEIGEMS
                     LRIQATLLRVLEERVVRRVGETEPRPVDVRILCATNRNLQDEVNAGRFRRDLYYRISG
                     MTIAIPPLRERRDAIEAIARAFAALSAARARRVPPTFTDEAIQALRSYPWPGNIRELR
                     NSIERAVLLAGAGPIRPQELGLAADYSPEAPTPSVGVPTVPKPDERTGPYGSERPTMD
                     SRDLSVEVAELERRRIVEALDRFGGNQTRAARALGMSRTTLVARMEAYALRRPRK"
     gene            1756..>2168
                     /locus_tag="VFV99_01750"
     CDS             1756..>2168
                     /locus_tag="VFV99_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5058047.1"
                     /translation="MKTRAAISFEPGKPLVVEEVDLAGPRAGEVLVELKATGICHTDE
                     FTRSGGDPEGLFPVILGHEGAGVVVEVGPGVTSVAVGDHVIPLYTPECRQCKSCLSHK
                     TNLCTAIRATQGKGVMPDGESRFSLRGQKVHHYMG"
BASE COUNT          360 a          743 c          739 g          326 t
ORIGIN      
        1 gcagatccgc gttaggcaga cgtacctcgt cgtgttgcta gacgatcgcg cggtgcaact
       61 cttgccttcc acgggcaggg tctcgatcgg gcgagcaacg gatgcgcagg tcgtgatcca
      121 acacccgtcg gtttcgcgcg agcacgccgt gctcgacgtc gagcagctga cgctcaccga
      181 cgtcggcagc cgcaacggca cgtacgtcgg cggccagcgc atcacgccga acacgccaat
      241 tcatctcggt cgtggcgaga ccatcgtggt gggtgaagtg acgatgttcg tgcagcaaga
      301 tggcgcgagc gctccagcgc tcgcagcgcc gattcccgat gcgccgctcg agctgcgcct
      361 tgtcgatgaa acggcgcgcg cggcgcgcca cgggtcgccg ttcgttcacg tccgcgtaca
      421 cgtgccggag ccgcaagccg atgctgcacg cgaagtcctc gccgacgcgc ttcgcagctc
      481 ggacgtcatg agtgaagagg cgccgggccg ctttcagctg ctgctgcccg cgatcacgag
      541 cgagcgcggc aggcccgtca tccaacggtt ggtgcagacg ctcgatcgca gcggcatcga
      601 ggcacgcacg ggcatcgcgt gctggccgca agatggtgtg accgcggcgc agctgtccgc
      661 gcacgcgcgc gagctggtgg cggcgaaggc ccgcaacgcg tcgccgatgg acgaggtgat
      721 gcggctcgcg gcgagcgtcg ccagcaccga gatcacggtc ctcatcaccg gcgagaccgg
      781 tgtcggcaag gagctgatcg cggagatgat ccaccgtctc tcgccgcgca gtcaggctcc
      841 gttcatgcgg atcaactgcg cggcaatcgc ggagccactt ctcgaaagcg agctgttcgg
      901 ccacgcgaag ggctcgttca cgggcgccga cagcgcgcgc attggtctgt tcgaggccgg
      961 cgccggcggc acggtgctgc tcgacgagat cggcgagatg agcctacgca tccaggccac
     1021 gctgctccgc gtgctcgagg agcgcgtcgt gcgccgcgtt ggcgagaccg aaccgcggcc
     1081 ggtcgacgtg cgaatcctgt gcgcgacgaa ccgcaacctc caagacgagg tcaacgcggg
     1141 ccgcttccgc cgcgacctct actaccgcat ctcgggcatg acgatcgcga ttccaccgct
     1201 gcgcgagcgg cgcgacgcca tcgaggcgat cgcacgtgcg ttcgccgcgc tatcagcggc
     1261 gcgtgcgcgc cgggtcccac cgacgttcac cgacgaagcg atccaggcgc tgcgctcgta
     1321 tccgtggccg ggcaacattc gcgagctccg caactcgatc gagcgcgcgg tgctactcgc
     1381 aggcgccggg ccgatccgcc cacaggagct cgggctcgcg gcggactaca gtcccgaggc
     1441 cccgacgccg tcggtcgggg taccgacggt gcccaaaccg gacgagcgaa ccggtccgta
     1501 cggctccgag cggccgacga tggactcgcg cgatctgtcg gtcgaagtcg ccgagctcga
     1561 gcgccgccgc atcgtcgagg cactcgatcg gttcggcggt aaccagacgc gcgcagcacg
     1621 cgcgctcggc atgtcgcgca ccacgctggt cgcgcgcatg gaagcctacg cgctgcgtcg
     1681 accgcgtaag taactcccgt cgcgaaagct cccggccggc cgctttgcat ggcgaaagcg
     1741 cgatacggtc gcggcatgaa gactcgtgca gcgatttcgt tcgaacccgg taagcctctc
     1801 gtagtcgaag aagtcgacct cgcaggtccc agggccggcg aagtcctcgt cgagctcaag
     1861 gcgaccggca tctgccacac cgacgagttc acgcgctcgg gcggcgatcc cgaaggcttg
     1921 ttcccggtga tcctcggcca cgaaggcgcg ggcgtcgtcg tcgaggttgg cccgggcgtg
     1981 acgtcggtcg ccgtcggcga tcacgtgatt ccgctctaca caccggaatg ccggcagtgc
     2041 aaatcgtgcc tgtctcacaa gacgaacctg tgcaccgcga ttcgcgcgac gcaaggcaag
     2101 ggcgtgatgc ccgatggcga gagtcgattc tcgctgcgcg gccagaaggt ccaccactac
     2161 atgggctg
//
LOCUS       DASUFX010000045         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1674138, whole genome shotgun sequence.
ACCESSION   DASUFX010000045 DASUFX010000000
VERSION     DASUFX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674138"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFV99_01755"
     CDS             complement(<1..248)
                     /locus_tag="VFV99_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058048.1"
                     /translation="MLARTMIATAVAFTIAVVLTPLLRNAARRRGYLDVPNEASSHIL
                     PTPRNGGFAIFAGIVAAFLLVRDLPGVAKLLGVVVANA"
     gene            292..1761
                     /locus_tag="VFV99_01760"
     CDS             292..1761
                     /locus_tag="VFV99_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058049.1"
                     /translation="MKAYIFFSVHEELFHRVAERLREHGVDELSGFAWSPYQLRKISG
                     RGIDYDSSLVFTRDMLPACDDGKPADLAWLQKRERELGVSIQRMLASERHLLAGRNFD
                     QLMRLAEVALREIGAAFDRVQPDFIFSEDVSCFHSYAHWVLARERGIPFWCIGTGRLP
                     NRLSVYSQRPQRYENVDALYREIVSRGLTADERKEAEDYVVSFRARPQRPTGMGTRAK
                     NPGFERSDARRFKNEVALYLSDRDNPVATPPLQAIGQRFRRIARVQLADLAGVFEPPE
                     PGEQYVLYPIHYQPEASTLVQAPMYLDQVQLLHDIAKSLPIGYRLYVKEHVSNRGRRP
                     LEFYQAIREIPAVRLLGPDADTWSLIQNAAAIAVITGTMGWEGLLFGKPVISFGDVYY
                     NVLPHVYRASEVPKDGWYELFARATSQHRDDHDALLALVSALHQRTWPGFMKNPNTFP
                     EVLADENVANITHALVDAMGLLTSQLRRPPRSEGAAPRT"
     gene            complement(1673..2929)
                     /locus_tag="VFV99_01765"
     CDS             complement(1673..2929)
                     /locus_tag="VFV99_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058050.1"
                     /translation="MRILQLSFGKPPHRFATVGGDVGMFQNFMALTELGHEVHVAVIG
                     PRTDNLDSDLRKRAAKVHFIEPRETPALLRPVERVLNPETFSLRFPRAHGYADALADL
                     AKQAAPDLIWADSTFTLAWAPRDTYPVVFGNYDFLFKLKSVRRETQRRVSLRDFGDPR
                     ALRRRFVRRPDAFSIAALEKFELEITSEAAHVMCVSASEAEFCRAHGISATHIPIVGP
                     TMPSPSLELPDRPPRFFLFGTHNTAHAAALSEIRRSLWPALERAGVRLEWHQTGKAPA
                     RRDDDWRWMERTFDHVHGFVADLGDVFALGDVSVVPYRHDTGFRTKFTVAAGYGVISA
                     GYTETYLCAPEFKPAHDCISEHDAEGLARAFTRIAQDREWRRSLGIAARKLYEREFTF
                     EAQLPRYAAVVEAATSAGPLRRRAHG"
BASE COUNT          544 a          955 c          990 g          520 t
ORIGIN      
        1 atggcgttgg caactacgac gccgaggagc tttgcaaccc cgggcaggtc acgcacgagc
       61 aagaacgcgg cgacgatacc ggcgaagatc gcgaagccac cgttccgcgg tgtcggcagg
      121 atgtgcgagc ttgcttcgtt cggcacgtcg aggtatccac gtcgccgcgc ggcgttgcgc
      181 aagagcggcg tgagaacgac ggcgatcgtg aacgccacgg ccgtcgcgat catcgtacga
      241 gcaagcatcg agaccccgta tcgttaactc ggttatcacc gcgcagtcag catgaaagcc
      301 tacatcttct tctccgtgca cgaggagctc ttccatcgcg tcgcggagcg tctgcgcgag
      361 cacggcgtcg acgagctcag tggtttcgcg tggagcccgt accagctgcg caaaatctcc
      421 ggccgcggca tcgactacga ttcgtcgctc gtgttcacac gcgacatgct gccggcatgt
      481 gacgacggca agcccgcgga tctcgcgtgg ctccagaagc gcgaacgcga gcttggcgtc
      541 tcgatccagc gcatgctcgc gtcggagcgg cacctgcttg ccggccgcaa cttcgaccag
      601 ctcatgcggc tcgccgaggt cgcgctgcgc gagatcgggg ccgccttcga tcgggtgcag
      661 ccggatttca tcttctcgga agacgtgtct tgctttcaca gctatgcgca ctgggtgctc
      721 gcgcgcgagc gcgggattcc gttctggtgc attggcaccg gacgattgcc gaatcggttg
      781 agcgtttact cgcagcgccc ccagcgttac gaaaacgtgg acgcgctgta tcgcgagatc
      841 gtgtcccgcg gcctgacggc cgacgagcgt aaggaagcag aggactacgt cgtgagcttt
      901 cgtgctcgac cgcaacgtcc gacgggcatg ggcacgcgtg cgaagaaccc cggattcgag
      961 cggtccgacg cgcgccgttt caagaacgag gtcgcgctct acctgtcgga tcgcgacaat
     1021 ccggtcgcga caccgccgtt gcaagccatt ggtcagcgct ttcgccgcat cgcgcgcgtc
     1081 cagctcgcgg atcttgcagg cgtgttcgag ccgcccgagc caggcgaaca gtacgtgctc
     1141 tatccgatcc actaccagcc ggaggcgtcg acactggtgc aagcgccgat gtacctcgac
     1201 caggtccagc tgcttcacga catcgcgaag agcttgccga tcggctaccg gttgtacgtc
     1261 aaggagcacg tcagcaaccg cgggcgccgg ccgctcgagt tctaccaggc gattcgcgag
     1321 atacccgccg tgcgattgct cggtcccgac gccgacacct ggtcgctgat ccagaatgca
     1381 gcagcgatcg cggtgattac cggcacgatg ggctgggaag gtctcctctt cggcaaaccg
     1441 gtgatcagct tcggcgacgt ctactacaac gtgttgccgc acgtgtatcg cgcgagcgaa
     1501 gtgccgaagg acggttggta cgagctgttc gcgcgcgcga cgtcacagca tcgcgatgat
     1561 cacgacgcgc tgctcgcgtt ggtctctgcg ctgcaccagc ggacgtggcc cgggttcatg
     1621 aagaacccga atacgtttcc ggaagtgctc gccgacgaga acgtcgcgaa catcacccat
     1681 gcgctcgtcg acgcaatggg cctgctgacg tcgcagcttc gacgaccgcc gcgtagcgag
     1741 ggagctgcgc ctcgaacgtg aactcacgtt cgtagagctt gcgtgccgcg atgccgagcg
     1801 agcgtcgcca ctcgcgatct tgcgcgatgc gcgtgaacgc gcgcgcgagg ccttccgcgt
     1861 cgtgttcgct gatgcagtcg tgcgcgggtt tgaactccgg cgcgcagaga tacgtctcgg
     1921 tgtagccggc gctgatcacg ccgtaaccgg cggcgaccgt gaacttcgtg cgaaagccgg
     1981 tatcgtggcg gtagggcacg accgaaacgt cgccgagcgc gaacacatcg ccgaggtccg
     2041 cgacgaagcc gtgcacgtga tcgaacgtgc gctccatcca gcgccaatcg tcgtctcggc
     2101 gtgcaggggc tttgccggtt tgatgccact cgagacgaac cccggcgcgc tcgagcgccg
     2161 gccacaagct gcggcgaatc tcggagagcg cggccgcatg cgctgtgttg tgagtgccga
     2221 acaaaaagaa ccgcggcggg cgatcgggta gctcaagcga cggcgagggc atcgtcggtc
     2281 cgacgatcgg aatgtgcgtc gcagagatgc cgtgggcgcg gcagaactcg gcctccgatg
     2341 cggacacgca catcacgtgc gcagcttcgc tggtgatctc gagctcgaat ttttcgagcg
     2401 ccgcgatcga aaacgcgtcc gggcgacgga cgaaccgccg gcgtagcgcg cgcggatcgc
     2461 cgaagtcgcg caggctgact ctgcgctgcg tctcgcgacg cacggacttc agcttgaaca
     2521 ggaagtcgta gttgccgaat accactggat acgtgtcgcg cggcgcccac gccagcgtga
     2581 acgtactgtc ggcccagatc aggtcgggcg ccgcctgctt cgcgaggtcg gcgagcgcgt
     2641 cggcataacc gtgtgcgcgc ggaaaccgca gcgagaacgt ctctgggttc agcacgcgtt
     2701 cgaccggtcg caacagtgcc ggtgtttcgc gcggctcgat gaagtgaacc ttcgcggcgc
     2761 gcttgcgcag atctgagtcg aggttgtcgg tccgcggacc gatcactgcg acatgcactt
     2821 catggccgag ctcggtgagc gccatgaaat tctggaacat gccgacgtcg ccgccgacgg
     2881 tagcaaatcg atgcggcggc ttgccgaaag agagctggag gattcgcatt acgtactcgg
     2941 atgcgcacga taccagcgca cggtctcgtg caaaccgcgc tcgagagtcc aacggcgctc
     3001 gaaacccgt
//
LOCUS       DASUFX010000046         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_284885,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000046 DASUFX010000000
VERSION     DASUFX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284885"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFV99_01770"
     CDS             complement(<1..476)
                     /locus_tag="VFV99_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058051.1"
                     /translation="MTLHRLTDRRETKPDEHGAAIARRLVRAIVMRLAERSRDRVAEC
                     LVDLAHRVEPEVGRTVVKRPARDLGPDDAVVLAGAELGVVRVAGDGDVLGVRIVSDDG
                     SAVADGPRIVVDDLRAAEAARPAAFDLDARRRARGGRLVVSAAAGESETKSQTSEKG"
     gene            475..1161
                     /locus_tag="VFV99_01775"
     CDS             475..1161
                     /locus_tag="VFV99_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058052.1"
                     /translation="MVRSRDTDVGTCVRKVIEEESLPRAPAVLDGHLHGAVAFASWPR
                     GYLPVAPRRDAIMPQVLARFGDVQTFGFRANATIELNEALRGRWFIGVNSELGAGDGA
                     SLSYALRALIGHRSALSRITTSVAIGVGVSDLGTYAPRGLEIPLEERVQVRFGSTFVH
                     LWVEARSVFFSEMRMFDRPPLGIDEYTLGGGLSFEIGDRRIFAGAAYETRVAGTSTMF
                     LLGIPVGDGY"
     gene            1177..1626
                     /locus_tag="VFV99_01780"
     CDS             1177..1626
                     /locus_tag="VFV99_01780"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5058053.1"
                     /translation="MATRRRAPAKKKPRVSTHPAKKRTPESRAIITNLQKICLALPDA
                     TEQIAWGEPTWRVRGKIFAQLDDHHHGAAHVSVWLPAPDGAQAALIEAEPDFIWRPPY
                     VGHKGWIAVILDTDPDWDMVAALVAQAHALIAGPPKRRVTRAARPTR"
     gene            complement(1598..2014)
                     /locus_tag="VFV99_01785"
     CDS             complement(1598..2014)
                     /locus_tag="VFV99_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058054.1"
                     /translation="MQNDVVVIVTQAYGPKGDNLVGISDVTFDGHPAVTLKIRAGGRE
                     GLVHLSPIHGDSRKQGLTDIEPGTKCELICPVSNQPLDIVGEVEDGSGARYYAIYLTP
                     QLSRGSMVMVSDVWGHYHSRIIDDFELISAWAAQHE"
     gene            2185..3729
                     /locus_tag="VFV99_01790"
     CDS             2185..3729
                     /locus_tag="VFV99_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058055.1"
                     /translation="MTRSLAVAIGVVVLLFAGAAEAKIRVAVVAFEGDDNGAVQEVVT
                     DLLDGDYSVSGTKAVNRTVDKLGLDANMSDKDLKKLANELEVDAILRGDLTKKGARKL
                     LHVKVYLKGKKVRGFKVEFASVKSEKFKSALKDKIDEKLSGQEAAKKQVDEAPAEAAA
                     DEEDPIGGKKKGKKGAKTDMAAEKAALKTKSDAPAEEEATEKTENEDGKSDEEKQSGD
                     EEDPEGKAKKKVASADADEEDVGGVSKSVTVEKSSGGRSANTVAFRLDVGPSISSRKL
                     VFNSRVFEEAPKPYANNPVGGMRVGGELYPLAFANPHSFISGLGFAGHYDQTLKLNLE
                     SSVQIGTKFPVTQRNWSVGARFRIAFGSSARAPTVTLGGGYFHRNFSVNRSQLMTGNI
                     IDLPDVLYKGYDPGIEFRIPIIKQVALMFGGQLLLVTDTGQIQQLNQYGQAKVTSGQG
                     MAGLDIVIANRVGVKLAAEMAQFGFAFTGNGDQTFNRDKDPATPDVGGAADRYYGGSF
                     TLGVLY"
     gene            3760..4686
                     /locus_tag="VFV99_01795"
     CDS             3760..4686
                     /locus_tag="VFV99_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058056.1"
                     /translation="MEPRDKIVVAHPERKAQRTLQRLVGATLCPVEVVADLDALVAAL
                     NPSAIAVVDAGLAQTRPDLRMHPARAWIAVPGEGLTPADAASSTALLAAGWDHVVSHP
                     MPLLAEELIATVQKLLRKDVFGLEKYMAWGAEVRSYVLDDARDRDAAVAKLAADVVAV
                     GLPDRIGSVVSVIADELLANAIYSAPVDGSGARFRANEPRDQARALTGRDAVTVRWAT
                     DARYLAIEVRDQWGSIDVPDVAKQLATGGNHVSTDGEGGMGLSLAYACSNQLVIDCEP
                     RVKTEVIALLDVRYKPTELARTASFHAFTHPC"
     gene            4696..>6070
                     /locus_tag="VFV99_01800"
     CDS             4696..>6070
                     /locus_tag="VFV99_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEX5058057.1"
                     /translation="MQPDVLAPSDTFARRHIGPQKTETAEMLALLGYKSLDELAAATV
                     PPAIRLKKPLALGEPLGEHELIEEMKNLAADNQVMRSFIGQGYYDTIVPPVILRNVLE
                     NPGWYTQYTPYQAEISQGRLEALINFQTMIEDLTALPLANASLLDEGTAAAEAMHMCF
                     GHADGKRSVFWVHDSTHPQTIAVMRTRAEPTGIDLRVGTLAQITAGLDASVAGVLLSY
                     PSTDGQVIDLRDTIAKVKAAGASVACATDLLSLTLLTPPGELGADIAIGTAQRFGVPM
                     GFGGPHAAFLGARDEFRRQIPGRIIGVSRDAKGKPAYRLALQTREQHIRREKATSNIC
                     TAQVLLAVMSSMYAVYHGPIGLTHIAKRVRGFTAVVAAGLTKLGYKPRATTYFDTLRI
                     DLDAHTQAKVLARALERGLNLRRYDDGLGISCDETTSHADVHDLLEAFAPGELPFEIA
                     TLINSTEV"
BASE COUNT         1103 a         1936 c         2026 g         1005 t
ORIGIN      
        1 cccttttcgg atgtttggct tttggtctcg ctctcgccgg ctgcggcgga gacgacgagc
       61 cggccacccc gagcacgacg acgggcatcg agatcgaagg cagctggacg agcagcttcg
      121 gcggcacgga gatcatcgac gacgattctt ggaccgtcgg cgacagcgct tccgtcatcg
      181 ctgactattc gaacaccgag aacgtcgccg tcacccgcaa cccggacgac gccgagttca
      241 gccccggcaa gtacaaccgc atcgtctgga ccgaggtcgc gggcgggtcg ttttactact
      301 gtacgaccga cttcgggctc gacacggtgg gcgaggtcga cgaggcattc ggcgacgcgg
      361 tcgcgcgagc gttccgcaag gcgcatgacg attgcgcgta caaggcgccg cgcaatggcg
      421 gctccgtgct cgtcgggctt cgtctcgcga cggtcggtaa gccgatgcaa ggtcatggtt
      481 cgctcgcgcg ataccgacgt cggaacctgc gtgcgcaagg tgatcgaaga ggagtccctg
      541 ccgcgtgcac cggcggtact cgacggtcac ctgcacggcg cggtggcgtt tgcgtcgtgg
      601 ccgcgcggct acctaccggt ggcgccgcgg cgagacgcga tcatgccgca ggttctggcg
      661 cggttcggcg atgtccagac cttcggcttc cgcgctaacg caacgatcga gctgaacgag
      721 gcgctgcggg ggcgctggtt tatcggcgtg aacagcgagc tcggtgcggg tgacggcgca
      781 agcctgtcgt acgcgctgcg cgcgctgatc ggccaccggt cggccctctc tcgcatcact
      841 acgtcggtcg cgatcggtgt tggcgtcagt gatctcggaa cgtacgcgcc gcgcggtctg
      901 gaaattccgc tcgaagaacg tgtccaggtc cggtttggct ccacgttcgt ccatctgtgg
      961 gtcgaagcac ggtcggtctt cttctcggag atgcggatgt tcgatcggcc gccgctcggg
     1021 atcgacgagt acacgttggg tggcggtctg tcgttcgaga tcggcgaccg gcgcatcttc
     1081 gccggcgccg cgtacgaaac gcgtgtggca ggcacgagca cgatgttctt gctcggcata
     1141 ccggtcggcg acggctacta ggcttacgat agcgcgatgg caactcgtcg ccgcgcgccc
     1201 gcgaagaaga agccgcgcgt gtcgacgcat cctgcgaaga agcgcacgcc cgagagccgc
     1261 gcgatcatca ccaacctgca gaagatctgt ctcgcgcttc ccgacgcgac ggaacagatc
     1321 gcgtggggcg aaccgacgtg gcgcgtgcgc ggcaagatct tcgcgcagct cgacgatcat
     1381 catcacggcg cggcgcacgt ctcggtgtgg ctaccggcgc ccgatggcgc gcaggctgcg
     1441 ctgatcgaag cagagcccga tttcatctgg cggccgccgt acgtcggtca caaaggctgg
     1501 atcgcggtca tcctcgacac cgatccggac tgggacatgg tcgccgcgct cgtcgcacag
     1561 gctcacgcgt tgatcgcggg cccgccgaag cggcgcgtta ctcgtgctgc gcggcccacg
     1621 cgctgatcaa ttcgaagtcg tcgatgatcc gcgagtgata gtgaccccag acgtcggaga
     1681 ccatcaccat cgagccacgc gatagctgtg gcgtaaggta gatcgcgtag tagcgcgctc
     1741 cggatccgtc ttcgacttcg ccgacgatat cgagtggctg attggagacc ggacagatca
     1801 gctcgcactt cgtgccgggc tcgatatcgg tcaagccttg tttgcggctg tcaccatgga
     1861 tcggcgagag gtgcacgaga ccttcgcgcc caccggcacg gatcttcagt gtcaccgccg
     1921 ggtgcccatc gaacgtgacg tccgagatgc cgaccaggtt gtcaccttta ggcccatagg
     1981 cctgagtgac gatgaccaca acgtcgttct gcactgcgac atgcccacca gtaaccgtgc
     2041 ggtgtttgcc ggtaatgcga aggtttcggc gatcgacggg gtccacgcag agagctgaca
     2101 acgcattgag acttcttgca agagttgaca gcttacagag tgctgcaagg acccctcgcg
     2161 tgggtgtata taatctatcc tccgatgact cgatcactcg cagtcgcgat cggagtggtg
     2221 gtcctgctgt tcgcaggtgc cgccgaagca aaaatccgcg tcgcggtcgt cgccttcgaa
     2281 ggtgatgaca acggcgccgt gcaagaggtc gtcacggacc tgctcgatgg ggattactcc
     2341 gtctcaggta cgaaggcagt gaatcgcacg gtcgacaagc tcggcctcga tgcgaacatg
     2401 tccgataagg acctcaagaa gctcgcgaac gagctcgagg tcgacgcgat tctgcgtggt
     2461 gatttgacga agaagggcgc gcgcaagctc ctgcacgtga aggtgtatct gaagggcaag
     2521 aaggtgcgcg gcttcaaggt cgagttcgcg agcgtgaaga gcgagaaatt caagagcgca
     2581 ctcaaggaca agatcgacga gaagctctcg ggccaagaag cggccaagaa gcaggtcgac
     2641 gaggcgccgg ccgaggcagc agcggacgaa gaagatccga tcggcggcaa gaagaagggc
     2701 aagaagggcg cgaagacaga catggcggcc gagaaggccg cgctgaagac gaagtcggac
     2761 gcgccggccg aggaagaggc gaccgagaag accgagaacg aagacggcaa gtccgacgaa
     2821 gagaagcaga gcggcgacga agaagatccg gaaggcaagg cgaagaagaa ggtcgcgtcg
     2881 gcggatgcag acgaggaaga tgtcggcggc gtctcgaaga gcgtcaccgt cgagaagtcg
     2941 agcggcggtc gcagcgcgaa caccgtcgcg ttccgcctcg atgtcggccc gtcgatctcg
     3001 tcgcgcaagc tcgtgttcaa ctcgcgcgtg ttcgaggagg cgccgaagcc gtacgcgaat
     3061 aatccggtcg gcggcatgcg cgtcggtggc gagctgtatc cgctcgcgtt cgccaacccg
     3121 cacagcttca tcagtggcct cggcttcgcg gggcactacg accagacgct caagctaaat
     3181 ctggagagca gcgtgcagat tggcacgaag ttcccggtca cgcagcggaa ctggagcgtc
     3241 ggcgcgcgct tccgcatcgc attcggcagc tcggcacgcg caccgacggt gacgctcggc
     3301 ggcggttact tccaccgcaa cttcagcgtc aatcgttcgc aactcatgac gggcaacatc
     3361 atcgatttgc ccgacgtcct ctacaaaggc tacgacccgg gcatcgagtt tcgcattccg
     3421 atcatcaagc aggtcgcgct catgttcggt ggtcagctcc tgctcgtcac cgacaccggc
     3481 cagatccagc agctgaacca gtacggtcaa gcgaaggtca cgtcgggcca gggcatggcc
     3541 ggcctcgaca tcgtgatcgc gaatcgcgtc ggcgtgaagc tcgccgccga gatggcgcag
     3601 ttcggcttcg cgttcacggg caacggcgac cagacgttca atcgtgacaa ggatccggcg
     3661 acgcctgacg tcggcggcgc tgccgatcgc tactacggcg gctcgttcac cttgggcgtt
     3721 ctttactaac gaacgcctgc acggcatgct ggcgggcgag tggagccccg cgacaagatc
     3781 gtcgtcgcgc atcccgagcg caaagcgcag cgcacgctgc agcggctggt cggcgcgacg
     3841 ctgtgcccgg tcgaggtcgt cgcggatctc gatgcgctgg tcgccgcgct taatccatcg
     3901 gcgatcgcgg tcgtcgacgc ggggctcgcg cagacgcggc ctgacttgcg catgcatcca
     3961 gcgcgcgcgt ggatcgcggt gccaggtgaa gggctcacac ctgccgatgc cgcgagctcg
     4021 accgcgctct tggcggcggg ctgggatcac gtcgtgtcgc atccgatgcc actgcttgcc
     4081 gaagagctga tcgcgacggt gcagaagcta ttgcgcaagg acgtgttcgg cttggagaag
     4141 tacatggcgt ggggcgccga ggtgcgcagc tacgtgctcg atgacgcgcg cgatcgcgat
     4201 gcggcagtcg ccaagctcgc ggcggatgtc gtggcggtcg ggctaccgga tcggattggc
     4261 tccgtcgtca gcgtgattgc cgacgagctg ctcgcgaacg cgatctattc ggcgcccgtc
     4321 gacggcagcg gcgcgcggtt tcgcgcgaac gagcctcgcg atcaggcccg cgcgctgacc
     4381 gggcgcgatg cggtgacggt gcgctgggcc accgacgcgc gctatctcgc catcgaagtc
     4441 cgcgatcagt ggggctcgat cgacgtgccg gatgtcgcga aacagctggc gaccggcggc
     4501 aaccacgtgt cgacagatgg ggagggcggc atggggttat cgctcgctta cgcctgctcg
     4561 aaccagctcg tgatcgactg cgagccgagg gtcaagaccg aggtgatcgc cctgctcgat
     4621 gttcgctaca aaccgacgga gttggcccgt acagcgtcgt ttcatgcgtt cacgcacccg
     4681 tgctagggtg cgcccgtgca gccggacgtc ctcgctccat cggatacttt cgcacgccgc
     4741 cacattggac ctcagaagac cgagaccgcc gagatgctgg cgctgctcgg ctacaagtcc
     4801 ctcgacgagc tcgcagcggc gacagtcccg cccgcgattc gcctgaaaaa gccgctcgca
     4861 ctcggtgagc cgctcggcga gcacgagctg atcgaggaga tgaagaacct cgcggccgac
     4921 aaccaggtca tgcggtcgtt catcggccag ggctattacg acacgatcgt gccgccggtc
     4981 atcctgcgca acgtgctcga gaacccgggc tggtacacgc agtacacgcc gtaccaggcg
     5041 gagatttcgc aaggccggct cgaggcgctc atcaacttcc agacgatgat cgaggacctc
     5101 accgcgctgc cgctcgcgaa tgcatcgctg ctcgacgagg gcaccgcggc cgccgaagcg
     5161 atgcacatgt gcttcggcca tgccgatggc aagcgcagcg tgttctgggt ccacgactcg
     5221 acgcacccgc aaacgatcgc ggtgatgcgc acgcgcgccg agccgaccgg catcgacctc
     5281 cgcgtcggca cgctcgcgca gatcacggcc ggcctcgatg cctcggtcgc cggcgtgctg
     5341 ctctcgtatc cgtcgacaga cggccaggtc atcgacctgc gcgacacgat cgcgaaggtg
     5401 aaggctgccg gcgcgtccgt cgcgtgcgcc acggacttgc tgtcgctcac gctgctcacg
     5461 ccgccgggcg agctcggcgc cgacatcgcg atcggcaccg cgcagcgctt tggcgtaccg
     5521 atgggctttg gcggtccgca cgccgcgttc ctcggcgcgc gcgacgagtt ccgacgccag
     5581 attccaggtc gcatcatcgg cgtctcgcgc gatgcgaagg gcaagcctgc gtaccgcctc
     5641 gcgctgcaga cgcgtgaaca gcacattcgc cgcgagaagg cgacgagcaa catctgcacc
     5701 gcgcaggtgc tgctcgccgt gatgtcgtcg atgtacgccg tctaccacgg cccgatcggc
     5761 ctcacgcaca tcgcgaagcg cgtgcgcggg ttcacggcgg tcgtcgccgc cggtctcacg
     5821 aagctcggct acaagccgcg cgccacgacg tacttcgaca cgctgcgcat cgacctcgac
     5881 gcgcacacgc aagcgaaggt gctcgcgcgc gcgctcgagc gcggcctcaa cctccgccgc
     5941 tacgacgacg gcctcggcat ctcgtgcgac gagacgacga gccacgccga cgttcacgat
     6001 ctgctcgagg cattcgcgcc gggtgagctt ccgttcgaaa tcgccactct gatcaactcg
     6061 accgaggtgc
//
LOCUS       DASUFX010000047         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1813610, whole genome shotgun sequence.
ACCESSION   DASUFX010000047 DASUFX010000000
VERSION     DASUFX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813610"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="glgP"
                     /locus_tag="VFV99_01805"
     CDS             <1..951
                     /gene="glgP"
                     /locus_tag="VFV99_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5058058.1"
                     /translation="DLELIGGDPFSMTVAGLRMARIANGVAQLHGETARAMWKDIDGA
                     APIISVTNGVHAPTWQDARIRAALVPDKSREQQDNELWDAHETMKQELIAEIEQRTGI
                     KLAHQGLLIGFARRAAAYKRADLILGDEKGLRRLFDSGVQLVYSGKAHPRDLAGKSLV
                     GRLVEAEKRHPGRIVFLENYDMHLGALLTRGADIWLNNPRRPLEASGTSGMKAAMNGV
                     PNLSILDGWWPEGCEHGVTGWKIGDPDPKDDPFDEKDEKRVDHRDRELLHHVLEQEVI
                     PAHQNRARWTAIMRASIVMSQWRFSSDRMIEEYVQRVYRS"
     gene            967..1854
                     /locus_tag="VFV99_01810"
     CDS             967..1854
                     /locus_tag="VFV99_01810"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX5058059.1"
                     /translation="MRLVLVVGVVVVVVLAAHPAHATYSIVATDTATRQVGGAVTSCV
                     GTLSVSIVYGGVPGKGAVHAQAQLGGPGKNAAVMQLGMDVDPAAIITSITATAFDANA
                     QRRQYGIVDLMARSAGFTGTQTSPFTDDRQAQIGTYTYSVQGNILTSAAVLDQAAASF
                     QTQGCDLADKLMLALEAGAQNGEGDSRCTGSGIPSDSAFLEVDREGEAAGTYLRLDVT
                     NTSPQSPLVLLRAQYDAWRVTHPCPAPPMPDAGAGGDGGIDDMSDDGGGCCSGSGDSN
                     SWPLVLVVGVLRRRSRART"
BASE COUNT          380 a          695 c          704 g          322 t
ORIGIN      
        1 gatctcgagt tgatcggcgg cgatccgttc tcgatgacgg tggcggggtt acggatggcg
       61 cggatcgcga acggcgtcgc gcagctccac ggcgagaccg ctcgcgcgat gtggaaggac
      121 atcgacggcg ccgcgccgat catcagcgtc acgaacggcg tgcacgcgcc gacgtggcaa
      181 gacgcgcgca ttcgcgccgc gctcgtgccc gacaagtcac gcgagcagca agacaacgag
      241 ctgtgggatg cgcacgagac gatgaagcag gagctcatcg ccgagatcga gcagcgcacc
      301 ggcatcaagc tcgcgcacca aggcctcttg atcggcttcg cgcgccgcgc cgccgcatac
      361 aagcgcgccg acctcatcct cggcgacgag aaaggcctgc gccgcctgtt cgatagcggc
      421 gtgcagctcg tctactcggg caaagcacac ccgcgcgatc tcgcaggcaa gtcgctcgtc
      481 ggccgcctcg tcgaagcgga gaagcgccac ccggggcgga tcgtgttcct cgagaactac
      541 gacatgcacc tcggtgcgct gctcacgcgc ggcgccgaca tctggctcaa caacccgcgc
      601 cgtccactcg aagcctcggg cacgagcggc atgaaggccg cgatgaacgg cgtcccgaac
      661 ctctcgattc tcgacggttg gtggcccgaa ggctgcgagc acggcgttac cggctggaag
      721 atcggcgacc ccgatccgaa ggacgatccg ttcgacgaga aggacgaaaa gcgcgtcgat
      781 caccgcgatc gcgagctgct ccaccacgtc ctcgagcagg aagtcatccc ggcgcatcag
      841 aaccgcgcac ggtggacggc gatcatgcgc gcgtcgatcg tgatgtcgca gtggcggttc
      901 tcgtcggatc ggatgatcga agaatacgtg cagcgcgtgt atcgcagctg atgcgatact
      961 gagcccatgc ggctcgtgct ggtcgtcggc gtcgtcgtcg tcgtcgtgct cgctgcgcac
     1021 cccgcgcacg cgacgtactc gatcgtcgcg accgacaccg cgacgcgcca ggtcggcggc
     1081 gccgtcacgt cgtgcgtcgg cacgctgtcg gtcagcatcg tctacggcgg tgtgccgggc
     1141 aaaggcgccg tccacgcgca agcgcagctc ggcggcccgg gcaaaaacgc ggcggtcatg
     1201 cagctcggca tggacgtcga ccccgccgcg atcatcacgt cgatcaccgc gacggcgttc
     1261 gacgcaaacg cgcagcgccg ccagtacggc atcgtcgatc tgatggcgcg ctcggcgggc
     1321 ttcaccggca cgcagacgtc gccgttcacc gacgatcggc aggcccaaat cggtacgtac
     1381 acgtactcgg tgcagggcaa catcctgacg agcgccgcgg tgctcgatca ggcagcggcg
     1441 tcgttccaga cgcagggctg cgatctcgcc gacaagctga tgctcgcgct cgaagcgggc
     1501 gcgcagaacg gcgagggcga cagccgctgc acggggagcg gcatcccgtc tgattcggca
     1561 tttctcgagg tcgatcgcga aggcgaagcg gcgggaacgt atctgcggct cgacgtgacg
     1621 aacacgtcgc cgcagagccc gctcgtcctc ttgcgcgcgc agtacgacgc gtggcgcgtg
     1681 acgcacccgt gcccggcacc accgatgccc gacgcgggcg caggtggcga tggcggcatc
     1741 gacgatatga gcgacgacgg cggcggttgc tgctcgggct cgggcgatag caattcctgg
     1801 ccactcgtgc tcgtcgtcgg ggtgttgcga cgacgctcgc gcgcgcgaac gtgatcaacg
     1861 ctacggctgg tcgcttattc cataagaccg acgccacgcc tcatgcgttt cgttcggcgc
     1921 aaggagctag tcatggcgtt ctcaggaaac ggcatctatc acatcgtaat cgtgggtccg
     1981 tcggcatttc gcgtggatct gcggtcgcag gaagcatcga atcaccacgg cacggttcag
     2041 atctatcacc cgaatgacac cgcgtatcag atgtggaagt tcaagcagga tccgggcggc
     2101 a
//
LOCUS       DASUFX010000048         4688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1813617, whole genome shotgun sequence.
ACCESSION   DASUFX010000048 DASUFX010000000
VERSION     DASUFX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813617"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFV99_01815"
     CDS             <1..955
                     /locus_tag="VFV99_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5058060.1"
                     /translation="VLGVYFAKTDSSKPAAAAKIDPPAPVAKIAPPAPSAAPSVAPAL
                     QPKQEAKAEEPAPGNETEAKTIDVAPLVVEAKPEVKAETPVAADTKPPAEAKPPEAVA
                     EQPKPVEPQVVIEPGSPASRFLGTAPTQPTVPTVSKKLKPAPAPTAVSDLPPPAKVAE
                     PTPAAPVAVKPVAKPVAAKPVKIAKKTDAQLRAEKRVAATRAAQASIAAAKARSTKKV
                     AAVEDDEADDTDEAEEPAKKPEPKPAAKPGGKGVLQIASTPAMEVWVDGRNSGAQTPV
                     RIILLTGKHKVTLFDKQNAKTKTFEIEIRPDETTKVTKNYE"
     gene            complement(1000..2406)
                     /locus_tag="VFV99_01820"
     CDS             complement(1000..2406)
                     /locus_tag="VFV99_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058061.1"
                     /translation="MRHSIAALLLLSATAYAEDTTPPPEEKATEPDVAKKQTAAIEPL
                     SAQETVSVTKDAPAQTGKGLEVGDVRFELHGYARMPLAAQTTPREPYLVDNDYYLSGF
                     SYTRLYEPDWSELFFSAKRGNYKAEFGLFASLYSDYASARLENQLGVAQASVTAKKFL
                     DVEPLTVQLGVFWDRFGYIEPYDTYVYGRTHQGGVKVAYDLPGGGRVQTGIGIHQAQL
                     QQNLGLTPVAHVAGAYPIPGVPGLELGVYTLRTWTRDKRQLSPIKDGTMWVTGLDARY
                     KLPNERGTAYLAFGYYNMDHVLYLAPALEVMHSTGGRGLTENFLGLDASDDGSGHMYT
                     LAGDVKTHITDRIGARAFGMATWVRSPQVDEMDPLANRDRRLYLKWGLEPSYKIIPQL
                     TASARYDRVILDVYDSENSFRVLSPRLTFPLDTWGELFFMYSHYWYGDKVRLRPGQVP
                     LETMPDSDVFKLQAQVVW"
     gene            complement(2409..3440)
                     /locus_tag="VFV99_01825"
     CDS             complement(2409..3440)
                     /locus_tag="VFV99_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5058062.1"
                     /translation="MIRNLSIILLATIGLGLLPACNKQEGPQVAFLLSTLQEERYQKD
                     VKYFEARAKELGLRVVTLAADNDNAKQIAQVEDVLTQGAKVLVIQPTDSQAASSYVRL
                     AHERGAKVVAYDRAIIAPDLDYYVSHDSYRVGVLQAQAALQATRNKGKYVILSGQAGH
                     SVATEITRGYKDTLAPYIARGEIEIVLEQNHSAWSPEQALRTVEDALTRTGNHVDAIL
                     ANNSGMARGAVQAVSAAGLQHVFIAGADADAANVNFVCQGKQTIEVLKDIQPLARTAA
                     ETAKKLFDGESPMAGSSIQFAGKPVPVAAVRVEVITPDTVKSLIVDSGFLTADELPAC
                     KNRLAAAAR"
     gene            complement(3437..4594)
                     /locus_tag="VFV99_01830"
     CDS             complement(3437..4594)
                     /locus_tag="VFV99_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner-membrane translocator"
                     /protein_id="HEX5058063.1"
                     /translation="MSRKLRPEVDRQRPRIDLNAWTMAGVLALMWGALALMPATRGVF
                     LTQGNIGTLLTQSSVLLVISVGMTLVILIRGIDLSVGAGVALTGVVAALCQIKLGLPA
                     PVAVAAAVAIGAALGAAHGWLTWLGIPAFIVTLAGFKYYRGGAILLADAKGLSPMRDD
                     FAVLAGTVPVAATWAIILIALGVGIALTLRDAQRRKQFGLEPTTTGVVIARVAGQLLL
                     AGLLLGVFGGRGVPVPVLVAGAVALFGIFVTKRTRFGRHLYAIGGNPEAARLSGIDVH
                     RATIGVYVIMGVLTAIAGVLLAARVNGVTPGSQGNLLELDAVTAVVVGGTSLLGGRGQ
                     VVGTVLGTLVFATLANGMNLLRIDSNWQLCCTGAVLIVAVLVDVLSKGKRS"
BASE COUNT          847 a         1528 c         1613 g          700 t
ORIGIN      
        1 cgtgctcggc gtctacttcg cgaagaccga ctcgtcgaaa cccgccgcag cggcgaagat
       61 cgacccgccc gcaccggtcg cgaagatcgc gccgcctgca ccgagcgctg cgccgagtgt
      121 cgcgccggcc ctgcaaccga aacaagaagc gaaggccgaa gagccggcac ctggcaacga
      181 gaccgaagcg aagacgatcg acgtcgcgcc gctggtggtc gaggcgaagc ccgaggtgaa
      241 ggcagaaacg ccagtcgctg ccgacacgaa gccgcctgcg gaagcgaagc cgccagaagc
      301 ggttgcggaa caacccaagc cggtcgagcc gcaagtcgtg atcgagccgg gatcgccggc
      361 gagccgcttc ctcggcaccg caccgacgca gcccacggtt ccgaccgtga gcaagaagct
      421 gaagccagcg ccggcaccga ctgccgtttc tgatctgccg ccgcccgcga aggtcgccga
      481 acccacgccc gcggctccgg tcgctgtgaa gccggtcgca aaaccggtcg ccgcgaaacc
      541 ggtaaagatc gcgaagaaga ccgacgcgca actgcgagcc gagaagcgcg tggctgcgac
      601 gcgtgccgcc caggcgtcga ttgctgcggc gaaggcgcgc tcgaccaaga aggtcgcagc
      661 tgtcgaggac gacgaagccg acgacaccga cgaggccgag gagccggcga agaagcccga
      721 gcccaagccc gctgcgaagc cgggcgggaa gggcgtgctg cagatcgcga gcacgcctgc
      781 gatggaagtc tgggtcgacg gccgcaactc gggtgcacag acgccggtgc ggatcattct
      841 gctcaccggc aagcacaagg tcacgctgtt cgacaagcag aacgcgaaga ccaagacgtt
      901 cgagatcgag atccgccccg acgagacgac gaaggtcacc aagaactacg agtagctgcc
      961 acgtggcacg agcctggggg cgcgtggcaa cgccggtagc taccagacga cctgggcctg
     1021 cagcttgaac acgtcgctat cgggcatcgt ctcgagcggc acctgaccgg ggcgcagccg
     1081 caccttgtcg ccgtaccagt agtgcgaata catgaagaac agctcgcccc aggtgtcgag
     1141 cgggaacgtc agccgcggcg atagcacgcg gaagctgttc tccgagtcgt agacatcgag
     1201 gatgacgcgg tcgtagcgcg ccgatgcggt gagctgcgga atgatcttgt agctcggctc
     1261 gagaccccac ttgagataga gcctgcgatc gcgattcgcg agcggatcca tctcgtcgac
     1321 ttgcggcgag cgcacccacg tcgccatgcc gaacgcgcgc gcgccgatgc ggtcggtgat
     1381 gtgcgtcttc acgtccccgg cgagcgtgta catgtggccc gagccgtcgt cgctcgcgtc
     1441 gaggccgagg aagttctcgg tcaggccgcg gccgccggtc gagtgcatca cctcgagcgc
     1501 gggcgcgagg tagagcacgt ggtccatgtt gtagtagccg aacgcgaggt acgcggtgcc
     1561 gcgctcgttc ggcagcttgt aacgcgcgtc gaggccggtg acccacatcg tgccgtcttt
     1621 gatcggcgag agctggcgtt tgtcacgggt ccacgtgcgc agcgtgtaca cgccgagctc
     1681 gaggccgggg actccgggaa tcggatatgc gcccgcgacg tgcgcgaccg gcgtgaggcc
     1741 gaggttctgc tgcaattgcg cctggtggat gccgatgccg gtctgcacgc ggccaccgcc
     1801 cggcaagtcg tacgcgactt tgacgccgcc ctgatgcgtg cggccgtaga cgtaggtgtc
     1861 gtacggctcg atgtaaccga agcgatccca gaacacgccg agctgcacgg tgagcggttc
     1921 gacatcgaga aacttcttcg cggtgaccga cgcctgcgcg acgccgagct ggttctcgag
     1981 ccgcgccgac gcgtagtccg agtacagcga cgcgaacagg ccgaactccg ccttgtagtt
     2041 gccgcgcttc gccgagaaga acagctcgga ccagtcgggc tcgtagagac gcgtgtacga
     2101 gaagccggag aggtaatagt cgttgtcgac gagatacggc tcgcgcggcg tagtttgtgc
     2161 cgcgagcggc atgcgtgcgt agccgtggag ctcgaagcga acgtcaccga cttcgaggcc
     2221 tttgccggtc tgcgccggcg cgtccttcgt gacgctgacg gtctcctgcg ccgagagcgg
     2281 ctcgatcgca gcagtctgct tcttcgcgac gtcgggctct gtcgccttct cctccggagg
     2341 tggcgtcgtg tcctccgcgt acgcggtggc agacagcaac agaagtgcag cgatcgagtg
     2401 gcgcatggtt acctcgcagc agcagcgagc cggttcttgc aggcaggcaa ctcgtcggcg
     2461 gtcaagaagc cgctgtcgac gatgagcgac ttcacggtgt ccggcgtgat cacctcgacg
     2521 cgcaccgcgg cgacgggcac cggcttgccg gcgaactgga tcgacgagcc cgccattggc
     2581 gattcgccgt cgaacagctt cttcgcagtc tcggccgcgg tccgcgcgag cggctggata
     2641 tccttgagca cctcgatcgt ttgcttgccc tggcagacga agttcacgtt cgccgcgtcg
     2701 gcatcggcgc cggcgatgaa gacgtgctgg aggccggccg cgctaacggc ctgcaccgcg
     2761 ccacgcgcca tgcccgagtt gttcgcgagg atcgcgtcga cgtggttgcc ggtgcgggtc
     2821 agcgcatcct cgacggtgcg gagcgcttgc tcgggcgacc acgcgctgtg gttctgctcg
     2881 aggacgatct cgatctcgcc gcgcgcgatg tacggcgcga gcgtgtcttt gtagccgcgc
     2941 gtgatctcgg tcgcgaccga gtggccagcc tgaccgctca ggatgacgta cttgcccttg
     3001 ttgcgggtcg cctgcagcgc ggcctgcgcc tgcagcacac cgacgcggta gctgtcgtgc
     3061 gagacgtagt agtcgaggtc cggcgcgatg atcgcacggt cgtatgcgac gaccttcgcg
     3121 cctctttcgt gcgcgaggcg cacgtagctc gatgcggctt gcgaatcggt cggctggatc
     3181 acgagcacct tcgcgccttg cgtgaggacg tcttcgacct gcgcgatctg cttcgcgttg
     3241 tcgttgtcgg ccgcgagcgt cacgacgcgg aggccgagct ccttcgcgcg cgcttcgaag
     3301 tacttcacgt ccttctggta acgttcctcc tggagcgtcg agagcagaaa tgcgacctgc
     3361 gggccttcct gcttgttgca agcggggagg agacccaaac caatcgtcgc gaggagaatg
     3421 atggagagat tccggatcac gagcgcttac ctttcgagag aacgtcaacc agcacggcaa
     3481 cgatgagcac cgcaccggta cagcacagct gccaattgga atcgatacgc aacaggttca
     3541 tgccgttggc gagcgtcgcg aacacgagcg tgccgagcac ggtgccgacg acctggccgc
     3601 gaccgccgag cagcgacgtg ccgccgacga cgacggcggt caccgcgtcg agctcgagca
     3661 agttgccctg gctgccaggc gtgacgccgt tgacgcgcgc cgcgagcaac acgcccgcga
     3721 tcgcggtgag gacgcccatg atcacgtaga cgccgatcgt cgcgcgatgg acgtcgatgc
     3781 ccgagaggcg tgcagcttcg gggttgccgc cgatcgcgta gagatggcgg ccgaaccggg
     3841 tgcgcttggt gacaaagata ccgaacaggg cgactgcgcc agcgacgagc accggcaccg
     3901 gcacaccgcg tccgccgaac acaccgagca agaggccggc gagcagcagc tggccagcga
     3961 cgcgcgcgat caccacgccc gtcgtcgtcg gctcgaggcc gaattgcttg cggcgttgcg
     4021 cgtcgcggag cgtgagcgcg atgccaacgc cgagcgcgat gaggatgatg gcccacgtcg
     4081 cggcaaccgg cacggtgccg gcgagcacgg cgaagtcgtc gcgcatcggc gacagtcctt
     4141 tcgcgtcggc gagcaggatc gcgccgccgc ggtagtactt gaaaccggcg agcgtgacga
     4201 tgaacgctgg aataccgagc caggtcagcc agccgtgcgc ggcgccgagc gcagcgccga
     4261 tcgcgaccgc cgcggcaacc gcgacaggag cgggcaggcc gagcttgatc tgacacagcg
     4321 ctgcgacgac gccggtgagc gcgacgcccg cgccgaccga cagatcgatg ccgcggatca
     4381 ggatgacgag cgtcatgccg accgagatca cgagcagcac cgagctctgc gtgagcagcg
     4441 tgccgatgtt gccttgcgtg aggaacacgc cgcgcgtcgc cggcatcagc gcaagagcgc
     4501 cccacatgag tgcgagcacg ccggccatcg tccatgcgtt cagatcgatg cgcggacgct
     4561 gccgatcgac ctcgggcctc aacttacgcg acacggatcg cctcctgcgc aagcgcggcg
     4621 ccgacagtgc cgaccgcgag ctgcatgatc gcaagctgcg tgatgtgatc acgctcgaga
     4681 gttcccgc
//
LOCUS       DASUFX010000049        17104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1674366, whole genome shotgun sequence.
ACCESSION   DASUFX010000049 DASUFX010000000
VERSION     DASUFX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674366"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFV99_01835"
     CDS             complement(<1..305)
                     /locus_tag="VFV99_01835"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058064.1"
                     /translation="MQGDLAQIDALWRAANYVSVGQIYLLDNPLLRKPLAREHIKPRL
                     LGHWGTTPGLTFIYSHLSYAIKKHDLDVLYVTGPGHGGPGLVGQAYLEGTYSEVYPN"
     gene            644..2206
                     /locus_tag="VFV99_01840"
     CDS             644..2206
                     /locus_tag="VFV99_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX5058065.1"
                     /translation="MALRNYMVGLLPLLAASCLVGPDYVRPKIVLNASWSEAQDARLG
                     TDPVDVVWWRSFNDPILDQLIEAAYHQNLSLQIAGLRIYEARAQLGVAIGRWWPSNVD
                     PIATGTAVGLSEHGPNQAIADKRFGQYQVGFDAVWELDIWGKYRHGIRAADAEWMATV
                     ADYDDALVSLSAEVARTYVLIRMFEVLIAQARGNVAIQEEGLQIAEARFKNGATSELD
                     VTQARNLLETTRATIPDLQMGQTQAENALSTLLGQPTGHVRAVLGNPADIPLPPVKVA
                     VSVPAEMMRRRPDIRAAELRAIAQCNRVGVAKSELYPSFVLFGSVGTQTSSNGGMPSN
                     NSTLSNLFGPGSLVYTAGGSLFWPILQYKRIRNNVRVEDARFQQTLVGYVDTVLRAAQ
                     EVEDGMTGYLRQQEAATFEQNAVAAAETGVKIALIQYREGAVDYTRVLDTQRSLLDSQ
                     QRLANTRAASITNLIALYKALGGGWESRQGDPVINDHNRIEMESRTSWGGYLETPHES
                     TTFKTESAPSRR"
     gene            2235..3281
                     /locus_tag="VFV99_01845"
     CDS             2235..3281
                     /locus_tag="VFV99_01845"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5058066.1"
                     /translation="MSKAVHKPYRKWTIVVVALLLVGFFGFRYWKAKRSELPKGIASG
                     NGRIESQHVDVSSKLPLKVKELNVQEGDLVKPGQIVGQMDTVTLEAELAEANQKVAAA
                     REALAVAKAGITRSKSQIELAKIEKERAKRLVAERAGSQRDYDVRSTALKTTTAGLAE
                     STAQLAVAQENVKVAEANVVTIQSRIDDATLRSPVLGRVLYRLAEPGEVLGPGGKALT
                     LVNLENIYMEIFLPSAQAGALKVGADARVTIDAAPGRVAQGTVSFVSPEAQFTPKQVE
                     TKSEREKLMFRVKIQIPQELVTRYVEQIKTGVRGVGYVKIRDDAQWPDWLQKNLVKQS
                     APTEQAPPPTTSHR"
     gene            3290..6037
                     /gene="rbbA"
                     /locus_tag="VFV99_01850"
     CDS             3290..6037
                     /gene="rbbA"
                     /locus_tag="VFV99_01850"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEX5058067.1"
                     /translation="MATPTASAAGPVVTIEDVTHLYGKVVAVDHLSLEIPSGLMVGII
                     GPDGVGKSTLLGLVAGAKTLQSGHIDVLGGDIASARHRREACPRIAYMPQGLGKNLYM
                     ELSVYDNVDFMAQLFGLSEYERQVRIKELLEATGLAPFHARPAGKLSGGMKQKVSLCG
                     ALVHDPDLLILDEPTTGVDPLSRRQFWTLIDSIRAGRPSMSVVISTAYMDEAEQWDWI
                     VAMDAGKVLATGTPRELMQRTGTTNLEQCFIALLPEEKRMGHTELVIPPFVPSGEYAI
                     EAQDLTKRFGAFTAVDRVTLKIEKGEIFGFLGSNGSGKSTTMKMLTGLLPPSEGTATL
                     FGVSVQAGSMEVRNNLGYMTQAFSLWEELTIRQNLALDARLYHLSDEKAKERIEELVD
                     RFGLRDHIDALAGDLPMGLRQRLSLAVAVLHEPKLLILDEPTSGVDPVARDSFWELLI
                     DLSRNEGVTIFVTTHFMNEGMRCDRISLMNAGKVLAADAPQKLIEERGAKNLEDAFIG
                     YMEDAIEETGTPKGTTEVPGPSESAKGKRAVLPLQVRRMLAYSRNEAIQIQRDPVRLA
                     FAFIGSALLMLVFGFGITTDVEHIRLTTLDHDRTPTSRAYLEQFEAIPRYFTPTSPIF
                     SDEAGLKRLRSDEASVIVEVPPHFGRDALRGASPDVLAQVDGAMPFRAETISQYVQGV
                     HDKALQDPATGLDVGPKKYQASIQERYMYNPTFESIYTIVPSVPALLLILIPAILMTV
                     SIVREKELGSIINFYVTPTGRLEYLLGKQLPYVAIGMINFFILALIASIVFGVPVKGS
                     FLVLTLCTLLYVIVTTGIGMVTSTFTKTQVAAVFITAILTIQPTVQFAGLLQPVSTLE
                     GSAKAIGSVWPTTYYMHSSVGAYTKGLAPGLMTHDLLILGIAIPILLVISMIGLKKQS
                     K"
     gene            6039..7163
                     /locus_tag="VFV99_01855"
     CDS             6039..7163
                     /locus_tag="VFV99_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5058068.1"
                     /translation="MASGANIWNLGLKELRSLLSDAVLMLFVVYAFTWTVYSQATGTS
                     NEVNNASIAFADEDGSALSKELFNAFYPPRFQRPIPIEPYQVEDAMDRGRFMFVVTIP
                     PQFESDLRAGRHPAIQVSIDATAMQQAGIGSGYIKKIVNQRVASFMHREDPTVEAPVE
                     LVVRKMFNPNGVSAWFTSVVAIINQLTLLTIVLTGAAVIREREHGTLEHLLVMPITAF
                     EIAMAKVWANGLVILIATGVSLLLVVQMVLKVPFAGSHLLWFVGVLLYLFFATALGMF
                     LGTISRSMAQFALLIVLVVVVLQMLSGGSTPVESQPGWLQKLTYLLPTRHFVSFSQVI
                     IYRGGGLGAVWKQFTVVGAVGLAFFVYSVSRFRQSIAVTK"
     gene            7182..8837
                     /gene="pgi"
                     /locus_tag="VFV99_01860"
     CDS             7182..8837
                     /gene="pgi"
                     /locus_tag="VFV99_01860"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5058069.1"
                     /translation="MPMSTLTSSPAWKALVEHKGAIASAHLRDLFATDPRRFKKMSKE
                     ACGIFVDISKHRATEETLRLLLALAKQQDVEGWRDKMFAGAKINGTEGRAVLHTALRN
                     RSNRPVLVDGKDVMPDINRVLGAMRSFTERVRSGAWKGHTGKTITDIVNIGIGGSDLG
                     PVMVTEALRPYWKPDLRVHFVSNVDGTHIAETLRRVDPERTLFIIASKTFTTQETLAN
                     ARAARAWLLEKLGGKQEAVAKHFVALSTNAKEVSAFGIDTANMFEFWDWVGGRYSLWS
                     AIGLSIACVIGMDRFEELLTGAHEMDEHFRTAPLAENLPVLLAMLGVWYASFWGAETH
                     AILPYDQYLHRFAAYFQQGDMESNGKGVDRHGNRITDYMTGPIVWGEPGTNGQHAFYQ
                     LIHQGTRLVPCDFIAPIETHNPVGNQHAILLANFFAQTEALMKGKTADEARAELVAQK
                     LPLDRVEQLVPHKTFPGNRPTTSILVQKITPKTLGALIAMYEHKIFVQGIVWDIYSFD
                     QWGVELGKQLATRILPELEAAGDVTTHDSSTNGLINLFKQRRG"
     gene            8906..9385
                     /locus_tag="VFV99_01865"
     CDS             8906..9385
                     /locus_tag="VFV99_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058070.1"
                     /translation="MPVDSQSYYTTYGPMVLRRCRKLLGDEHAARDAMHDVFIQVLSR
                     SEELVDQAPSSLLFRIATNVCLNRIRSRKRRPEDGDPDLLAEIATQTDPAARTAARSA
                     LDALFRQEPDDTALIAVLHLHDNMTLEEVAAEVGMSVSGVRKRLAKLRTKLHSLEVQ"
     gene            9382..10221
                     /locus_tag="VFV99_01870"
     CDS             9382..10221
                     /locus_tag="VFV99_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058071.1"
                     /translation="MSALPDWLVERAALDEVPAASRDRLERADQRELQQRIAGLRAEN
                     EAEMAAYPAAQAILLIESRAADESKRRAARRRQVQLRWVGILGTAMAAAAVLLVLGKH
                     DVSHPPTEAPVAAPEGDPIDITRAKGPTRLLAFRQVGDRAEQLVQDSVVRAGDLIQLS
                     YNAGGKPYGVIASIDGAGVVTLHFPASEDARPEGTALAKRPTALPNAYVLDDAPRFER
                     FFFITDDSPIDVSDTLAALRTFAARTDSATAKPDLPPGRGGWALRLRKPDHSSPTREE
                     RHD"
     gene            10214..11911
                     /locus_tag="VFV99_01875"
     CDS             10214..11911
                     /locus_tag="VFV99_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5058072.1"
                     /translation="MTSRLFIVCSAALVAVLAGVSPSYAKDTVEPRLRRYALVIGSNT
                     GGGEGRDKLRYADHDADTVADVLKQLGGVNNGDLAQLSEPNRDALDRELDTLSKRVRD
                     ERKRGQRVELVVYYSGHSDETGLLIGGARYDYARLRDRIRAVPADVKIAIVDSCASGS
                     FTRAKGGTKVPAFLHDTSNQVEGFAFLSSSSENEDAQESDRIKASFFTYFLVSGLRGA
                     ADRNRDGVITLSEAYQFSYEHTLGRTQNTSHGPQHPAYDMHLSGTGDVVITDLRTSDS
                     ALVLPADLKGHVTIVDENGRVTLELTKDAGEPLSLALPKDSYTVRVDNSNGSFEATIT
                     LTGTRSTELDKDALRRVAPEKTVARGLSIDEHADDDDDDDDHHDPRHRNLPISFGGGL
                     RVELPSTHQDFDALLSIGGTSREVHGHPVIGVAESGDLSIGMTLGDNVHFAYDMSLAV
                     GPGVFIGDNLQIGTTVGFGFSGITGGVLDFAWKIPTEAFVVLELSKDVRPMAYFRQNY
                     LFSSEARQNGSKMARWGDESEVGAGLHFSGALDGFLYGSVREMANERYWGLGMGAIL"
     gene            complement(11915..15421)
                     /locus_tag="VFV99_01880"
     CDS             complement(11915..15421)
                     /locus_tag="VFV99_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058073.1"
                     /translation="MTRFPSGEILLVGLMSADREGNASSEVDETVRERPSQPSQPSQS
                     IDEVTTPRESFFGADPELPHATLSTQTGDGIPDAPRRPTPVGFPIANWDRYELISYIG
                     RGGMGTVYKARDPQLRRFVALKFLTRSEPERARRFIAEARAQARVEHEHVCKVYEVGT
                     AQGIPYIAMQFIDGVTLGHAGEKMTTEDKVHTMRKVADAVHAAHQGGLIHRDLKRANI
                     MVERTDSGKWQPFVLDFGLAREVDADATLAGEVAGTPAYMAPEQARGERADKRTDVWG
                     LGATFYALLTGRPPNQGTTPEAFKSLVTEEVPSIRRVAPQIPSELATIVMKCLEQKPE
                     RRYATAAELGNDIRRYIAGEPIAARRRSLMYRASKRLRRSWGLTIALAVAAIAIVAVV
                     ALVLNAAHQRRLAEKRVQIAQRFGQEVARLETFMRQAYAAPLHDTRPERKHVLDRMTA
                     IQAEARELGDIATGPAEYAVGRGHLSLHDYKQAKPHLDAALAAGYDPPEARYALGLTL
                     AALYQEGVYDAERVRDSAVRDAKRAELKKTLRDPALAYLRAVGDSAPEAPAYAEALVA
                     FYEGDFATALTKAAAASQAAPWLYEAHKLAGDAFTARSSETSHTGNYAEAAADLERAG
                     EEYAQALAAAPSDAQLLDAECKRRGKKVSLAIAQRQLDQDMVDEAVEPCERARRADPD
                     AVTVMLREAYIIADAADFLASRRGTRPDEAMKRALPLIEAALAKDPRSAEAYYVRSVL
                     YAALSQAQADAREHPEQSTDQAIAALHKVTELDPSYADAYSDLAFILQQRAEREREAG
                     HDPRPILDEAVAYAERGIATSRQSSTLKTDLGTVWHARADYEAEHGIDPQASLGHAID
                     AFEASLAINPKNATALNNIGLAFHTRAYYRFEHGQVEGEADIMRAKEAYDRVLALGGT
                     DSKVLNNIGYLTIDRATYLARIGRDPRPVIEEGNSYLERALAANPAERFSYFNMAGLR
                     LIEAEYLVENGKDPAPALAKLETAIDADLERSGTPDPDIIAYRADGHVLAARWAISQK
                     ASPADAFAAADTAMKKAEQLDAMTGFVIDARIGLARWRAAWLVSQGQDASATLTRAKA
                     AASQAVEMVHDAPAAHLYVGEIATIEARSLKNAHGADAEKRRAAAIATAKRELEAAVA
                     GNKFYERKVTPLLTELAALASP"
     gene            complement(15478..16215)
                     /locus_tag="VFV99_01885"
     CDS             complement(15478..16215)
                     /locus_tag="VFV99_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058074.1"
                     /translation="MRYCSLFVCVVVGVAGCAEATDDADQYTSTSGGDTKADESEVTS
                     GLLYFYVSPLSPTSQGFGPLALRRANAKPSTLSPTYGAATLPEIRLADDALTTQAALD
                     AVIAIPYAGNENRVIAIIGGRNNSKPWENPRVDVVELYTPSQPVAIVDGQGLTGVAQR
                     DALYQLSLSGNKATGTLVNERTYPSASSVTYDYSAATNPSAAMTAVQGGALFTGKIDV
                     VCKKILGIWTKCDPPTGVHVAAYFNAN"
     gene            16356..>17104
                     /locus_tag="VFV99_01890"
     CDS             16356..>17104
                     /locus_tag="VFV99_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058075.1"
                     /translation="MREDSKLLKAVSALTSHVDGIDWDAAAVQADVDRMLEVVKQASA
                     SARAEALAVLVERLRRARLDDADGVAHAAISAGTLVEYGAPAEPLANVLLDKLPAVLA
                     AARRYADACLADLPPLGDNEEDDPASALVVIDGRGITLDVFRSHLDADRAGAGALHGL
                     NQWALAAIASLTRSRAALVRACANDELRRLARAMADSDAMWLDTLFGVELDARWLVLC
                     PLVERGFRISVDGIASNFELHTLVADTLIAQG"
BASE COUNT         2967 a         5498 c         5650 g         2989 t
ORIGIN      
        1 atgttcgggt agacctcgct gtacgtacct tccaggtacg cttgaccgac gagaccaggg
       61 ccgccgtgcc caggaccggt gacgtagagc acgtcgaggt catgcttctt gatcgcgtag
      121 ctcaggtgcg aatagatgaa cgtcaggcct ggcgtcgtcc cccagtgacc gagcaagcgc
      181 ggcttgatgt gctcgcgcgc gagcggtttg cggagcagtg gattgtcgag caggtagatc
      241 tggccgaccg acacgtagtt cgccgcgcgc cacaaggcat cgatctgcgc gaggtcgccc
      301 tgcaagcgtt ggctggtctg agtcggggac gcttcgcggg ctgtcggtcg ggtcgtcatt
      361 cgatcgctcc tcgtcacacg gttggcacta gcgacgcccg catgcgcgga gcaaagaacg
      421 cgcctgcttg catctacgcg tccgtcgcgc atagccgccg cagtcgacat tccgactaca
      481 ccgattccgc tcgcgctgat gttgctttgt acagcgtagt catcgtggca tcggggatcg
      541 ctcgtcgcgt gcgctacgca atgcgcgtga tcgatcgcag tgctgcatca cctcgcgctc
      601 gtcagactca cgaggcacat gcgttgcagc gcagcgccgc gacgtggcgc tccgaaacta
      661 catggtcggc ttgctgccac tgctggcggc ttcgtgcctc gtcggtcccg actacgtacg
      721 gccgaaaatc gtcctgaatg ccagctggag cgaagcgcaa gacgcacggc tgggcacaga
      781 tcccgtcgac gtcgtgtggt ggcgatcgtt taacgatccg atcctcgatc agctgatcga
      841 ggcggcgtac caccagaacc tgtcgttgca gatcgccgga ctgcgcatct acgaagcgcg
      901 tgcgcagctc ggcgtcgcga tcggcaggtg gtggccgtcg aatgtcgatc cgatcgcgac
      961 cggcaccgca gtcgggctca gcgagcacgg gccgaaccaa gcgatcgcgg acaaacgctt
     1021 cggtcagtac caggtcggtt tcgacgcggt gtgggagctc gacatctggg gcaagtaccg
     1081 ccacggcata cgtgctgctg atgcagagtg gatggcaacc gtcgcggatt acgacgatgc
     1141 gctcgtctca ctcagcgccg aggtcgctcg cacgtacgtg ctgatccgca tgttcgaagt
     1201 gctgatcgcg caggcgcgcg ggaatgtggc gatccaggaa gaaggactgc agatcgccga
     1261 ggcgcggttc aagaacggcg cgacgtccga gctcgacgtc acgcaggcaa ggaacctgct
     1321 ggagacgacg cgcgcgacga ttcccgatct gcagatgggc cagacgcagg ccgagaacgc
     1381 gctgagcacg ttgctcggtc aaccgacggg tcacgtacgc gcagtgctcg gcaatccagc
     1441 cgacattccg ctcccgcccg tgaaggtcgc ggtcagcgtg ccggccgaga tgatgcggcg
     1501 ccggcccgac atccgcgccg ccgagctacg tgcgatcgcg cagtgcaatc gggtcggtgt
     1561 cgcgaagtcc gagctctatc cgagcttcgt actgttcggt tcggtcggta cgcagaccag
     1621 cagcaacggt ggcatgccat cgaacaactc gacgctgtcg aacctcttcg gtccgggtag
     1681 cctcgtctac accgcgggcg gcagcctgtt ctggccgatc ctccagtaca agcggatcag
     1741 gaacaacgtc cgtgtcgagg acgcgcgctt tcagcagacg ctcgtcggct acgtcgacac
     1801 ggtgctcaga gcggcgcagg aggtcgagga cggcatgacc gggtacctgc gccagcaaga
     1861 agcggcgacg ttcgaacaga acgcggtggc agcggccgag accggcgtga agatcgcgct
     1921 catccagtac cgcgaaggcg cggtcgacta cacgcgtgtg ctcgacacgc aacgctcgct
     1981 gctcgactcg cagcagcggc tcgccaacac ccgtgcagct tcgatcacaa atctgatcgc
     2041 gctctacaag gcgctcggtg gtggttggga gtcgcggcag ggcgatccgg tcatcaacga
     2101 ccacaaccgg atcgagatgg agtcgcgcac gagctggggc ggctatctcg aaacgccgca
     2161 cgaatcgacg acgttcaaga cggagtcggc acccagccga aggtaggacg acgatgggca
     2221 aggttcgcga cacaatgtcc aaggcggtgc acaagccgta caggaagtgg acgatcgtgg
     2281 tcgtcgcact actgctcgtc ggcttcttcg gcttccgcta ctggaaggca aagcgctccg
     2341 agttgcccaa aggaatcgcg tcgggcaacg gccgcatcga gagccagcat gtcgacgtgt
     2401 cgtcgaagct gccgctcaag gtgaaggagc tgaacgtcca ggaaggtgat ctggtgaagc
     2461 cggggcagat cgtggggcag atggacacgg tcacgctcga agcggagctc gccgaggcga
     2521 accagaaggt cgcagctgcg cgcgaggcgc tcgcggtcgc gaaggccggc atcacgcgaa
     2581 gcaagagcca gatcgagctc gcgaagatcg agaaggagcg cgccaagcgg ctcgtcgccg
     2641 agcgcgccgg ctcgcagcgc gactatgacg tccgttccac cgcgctgaag acgacgacag
     2701 cgggtctcgc cgagtccacc gcgcagctgg cggtggcgca ggagaacgtc aaggtcgccg
     2761 aagcgaacgt cgtgacgatc cagagccgca tcgacgacgc gacgctgcgc tcaccggtac
     2821 tcggtcgcgt gctgtaccgg ctcgccgagc ccggcgaggt gctcggtccc ggcggcaagg
     2881 cgctcacgct cgtgaatctc gagaacatct acatggagat cttcttgccg tcggcgcagg
     2941 cgggcgcact caaggtcggc gcggatgcac gggtgacgat cgatgccgct ccgggccgcg
     3001 tcgcacaggg aaccgtgagc ttcgtgtcgc ccgaggcgca gttcacaccg aagcaagtcg
     3061 agacgaagag cgagcgcgag aagctgatgt tccgcgtcaa gatccagatc ccgcaggagc
     3121 tcgtcacgag gtacgtcgag cagatcaaga ccggcgtgcg cggcgttggc tacgtgaaga
     3181 tcagagatga cgcgcagtgg cccgactggc tgcagaagaa tctggtcaaa cagagcgcgc
     3241 cgaccgagca agcccctcca ccgaccacgt ctcaccgata ggagtcttcg tggcaacgcc
     3301 gactgccagc gctgcgggtc cggtcgtcac gatcgaagac gtcacccacc tctacggaaa
     3361 ggtcgtcgcg gtcgatcatc tctcgctcga gattccgagc gggctgatgg tcggcattat
     3421 cggtccagac ggcgtcggca agtcgacgtt gctcggcctc gtcgccggcg cgaagacact
     3481 gcagagcggt cacatcgacg ttctcggcgg cgacatcgca tccgcgcgtc accgccgcga
     3541 agcctgcccg cgcatcgcgt acatgccgca gggcctcggc aagaacctct acatggagct
     3601 cagcgtctac gacaacgtgg acttcatggc gcagctgttc gggctctccg agtacgagcg
     3661 ccaggtgcgc atcaaggagc tgctcgaggc gaccggactc gcgccgttcc atgcccgtcc
     3721 cgccggcaag ctgtcgggcg gcatgaagca gaaggtcagc ctctgcggtg cgctcgtgca
     3781 cgatccggat ctgttgatcc tcgacgagcc gacgacgggt gtcgatccac tgtcgcgccg
     3841 gcagttctgg acgttgatcg attcgattcg cgcgggtcgg ccgagcatga gcgtcgtgat
     3901 ctcgacggcg tacatggatg aagcggagca atgggactgg atcgtcgcga tggacgccgg
     3961 gaaggtgctc gcgaccggta ccccgcgtga gctgatgcag cgcaccggca cgacgaacct
     4021 cgagcagtgc ttcatcgcgc ttctgcccga agagaagcga atggggcaca ccgagctcgt
     4081 gatcccgccg ttcgttccat cgggcgagta cgcgatcgaa gcgcaggatc taacgaaacg
     4141 gtttggcgca ttcaccgccg tcgatcgcgt gacgctcaag atcgagaaag gcgagatctt
     4201 cggcttcctc ggttccaacg gctcgggcaa gtcgacaacg atgaagatgc tgaccggcct
     4261 gttgccgccg agcgagggca ccgcgacgtt gttcggtgtc tcggtccagg ccggaagtat
     4321 ggaggtgcgc aacaacctcg ggtacatgac ccaagcattc tcgctctggg aggaattgac
     4381 gatccgccag aacctggcgc tcgacgcacg gctctatcat ctgtccgacg agaaggcgaa
     4441 ggagcgcatc gaagagctgg tcgatcgctt cgggctgcgc gatcacatcg acgcgctggc
     4501 cggcgatctg ccgatggggt tgcgccagcg gttgtcgctt gcggtcgccg tgctccacga
     4561 gccgaagctg ctcatcctcg acgagccgac gtcgggcgtc gatccggtcg cgcgcgacag
     4621 cttctgggag ctgctgatcg atctgtcgcg caacgaaggt gtgacgatct tcgtgactac
     4681 acacttcatg aacgaaggca tgcggtgcga tcggatctcg ctgatgaacg cgggaaaggt
     4741 gctcgctgcg gatgcgccgc agaagctgat cgaggagcga ggcgcgaaga atctcgaaga
     4801 cgcgttcatc ggctacatgg aggatgcgat cgaggagacc gggacgccca agggaacgac
     4861 ggaggtgccc ggtccgtcgg agagtgcgaa aggcaagcga gcagtgctcc cgctgcaggt
     4921 gcggcggatg ctcgcgtact cgcgcaacga ggcgatccag atccagcgcg atccggttcg
     4981 cctcgcgttc gcgttcatcg ggtcggcgct gctgatgctg gtgttcggat tcggcatcac
     5041 caccgacgtc gagcacatcc gcttgacgac gctcgatcac gatcgcacac cgacgagccg
     5101 ggcgtatctc gagcagttcg aggcgatccc acgctatttc actccgacat cgccgatctt
     5161 ctccgacgaa gcgggcctga agcggctcag gtccgacgag gcatcggtga tcgtcgaggt
     5221 cccgccgcat tttggccgcg acgcactacg cggtgcgtct ccggacgtgc tcgcgcaggt
     5281 cgacggcgcg atgccgtttc gcgccgagac catctcgcag tacgtgcaag gcgtccatga
     5341 caaggcgttg caggatcccg ccaccggtct cgacgtcggc ccgaagaaat atcaggcatc
     5401 gattcaagag cgctacatgt acaacccgac gttcgagagc atctacacga tcgtgccgag
     5461 tgtgccggcg ctgctcttga tcctgatccc cgcgatcttg atgacggtca gcatcgtgcg
     5521 cgagaaggag ctcggctcga tcatcaactt ctacgtcacg ccgacgggcc ggctcgagta
     5581 tctgctcggc aagcagctgc cctacgtcgc gatcggaatg atcaacttct tcatcctcgc
     5641 gttgatcgcg tcgatcgtgt tcggcgtgcc agtcaagggc agcttcctgg tgctcacgct
     5701 gtgcacgctg ctctacgtga tcgtgacgac tggcatcggc atggtgacgt cgacgttcac
     5761 gaagacacag gttgccgccg tgttcatcac cgcgatcctg acgatccagc caaccgtcca
     5821 gtttgccggg cttctgcagc cggtgtcgac gctcgaaggc agcgcgaagg cgatcggctc
     5881 cgtctggccg acgacgtact acatgcactc gagcgtcggc gcctatacga agggccttgc
     5941 gccaggcctg atgacgcacg acctgctcat cctcgggatc gccatcccga tcctgctggt
     6001 catcagcatg atcggcctca agaagcagag caaataacgt ggcctcgggc gcgaacatct
     6061 ggaacctcgg actgaaggag ctgcgcagcc tcttgagcga cgcggtactc atgctgttcg
     6121 tcgtctacgc gttcacgtgg accgtgtatt cgcaggccac cggcacgtcg aacgaggtga
     6181 acaacgcatc gatcgcgttt gccgacgagg acggctcggc gctgtcgaag gagctgttca
     6241 acgcgtttta tccgccgcgg ttccagcggc cgattccgat cgagccgtat caggtcgagg
     6301 acgcgatgga tcgcggccga ttcatgttcg tcgtcacgat tccaccgcag ttcgagagcg
     6361 acctgcgtgc gggccgccat cccgcgatcc aggtcagcat cgatgcgacc gcgatgcagc
     6421 aagcaggcat cggctcgggc tacatcaaga agatcgtcaa ccaacgggtc gcatcgttca
     6481 tgcaccgcga agacccgacc gtggaggcgc ccgtcgagct cgtcgttcgc aagatgttca
     6541 atccaaacgg tgtctcggcg tggttcacga gcgtggtggc gatcatcaat cagctcacgc
     6601 tgctgacgat cgtgctcacc ggcgccgcgg tgattcgcga gcgcgagcac ggcacgctcg
     6661 agcacctgct ggtgatgccg atcaccgcgt tcgagatcgc gatggcgaag gtgtgggcca
     6721 acggcctcgt gatcctgatc gcgaccggcg tgtccctgtt gctcgtcgtc cagatggtgc
     6781 tgaaggtgcc gtttgccggc tcgcacctgc tgtggttcgt cggcgtgttg ctctatctgt
     6841 tcttcgcgac cgcgctcggc atgttcctcg gcacgatctc gcgatcgatg gcgcagttcg
     6901 cgctgttgat cgtgctcgtc gtcgtcgtgc tccagatgct gtctggcgga tcgactccgg
     6961 tcgagagtca gccaggctgg ctgcagaagc tcacgtactt actcccgacg cggcacttcg
     7021 tcagcttctc ccaagtgatc atctaccgcg gtggcggcct cggggccgta tggaagcagt
     7081 tcacggtcgt cggtgcggtt ggccttgcgt tcttcgtgta cagcgtgtcg cggttccgcc
     7141 agtcgatcgc ggtgacgaaa taggaaggct gtgatttgct catgcccatg tcgaccctga
     7201 cgagctctcc cgcttggaaa gcacttgtcg agcacaaggg cgcgatcgcg agcgcgcatc
     7261 tacgtgacct gtttgcgacg gacccgcgca ggttcaagaa gatgtcgaaa gaggcctgcg
     7321 gcatcttcgt cgacatctcg aagcaccgcg cgaccgagga gacgttgcgc ctgttgctcg
     7381 cacttgcgaa gcagcaggac gttgaaggct ggcgcgacaa gatgtttgcc ggcgcgaaga
     7441 tcaacggaac cgagggcagg gcggtgctgc acaccgcgtt gcgcaaccgc agcaatcggc
     7501 cggtgctcgt cgacggcaaa gacgtgatgc ccgacatcaa tcgcgtgctc ggcgcgatgc
     7561 gctcgttcac cgagcgggtg cggagcggcg cgtggaaggg gcacaccggc aagacgatca
     7621 cggacatcgt caacatcggc atcggcggtt cggatctcgg cccggtgatg gtgaccgaag
     7681 cgctgcggcc gtactggaag cccgatctgc gcgtgcactt cgtgtcgaac gtcgacggca
     7741 cgcatatcgc cgagacgctg cgccgcgtcg acccggagcg cacgctgttc atcatcgcga
     7801 gcaagacgtt cacgacacag gagacgctcg cgaacgcacg tgccgcgcgt gcgtggttgc
     7861 tcgagaagct cggtggcaag caagaggcgg tcgcgaagca cttcgtcgcg ctatcgacga
     7921 acgcgaaaga agtgtcggcg ttcggcatcg acaccgcgaa catgttcgag ttctgggact
     7981 gggtcggtgg ccgttactcg ctgtggagtg cgatcgggct ctcgatcgcg tgcgtgatcg
     8041 ggatggaccg cttcgaggag ctgctcaccg gcgcgcacga gatggacgag cacttccgca
     8101 ccgcgccgct cgccgagaat cttccggtgc tgctcgcgat gctcggcgtc tggtacgcga
     8161 gcttctgggg cgcggaaacc cacgcgatcc tgccctacga tcaatacctg catcgcttcg
     8221 ccgcctactt ccagcaaggc gacatggagt cgaacggcaa gggcgtcgat cgccacggca
     8281 atcggatcac cgactacatg acgggaccga tcgtgtgggg cgagcccggc acgaatggcc
     8341 agcacgcgtt ctatcaattg attcatcaag gcacgcggct cgtgccgtgc gacttcatcg
     8401 cgccgatcga gacgcacaac ccggtcggca atcagcacgc gatcttactc gcaaacttct
     8461 tcgcgcagac cgaagcactg atgaagggca agaccgccga cgaagcgcgc gccgagctgg
     8521 tcgcgcaaaa gctgccgctc gaccgcgtcg agcagctcgt gccgcacaag acgtttccgg
     8581 ggaaccggcc gaccacgtcg atcctcgtgc agaagatcac accgaagacg ctcggtgcgc
     8641 tcatcgcgat gtacgagcac aagatcttcg tgcagggaat cgtgtgggac atctacagct
     8701 tcgaccaatg gggcgtcgag ctgggcaagc aactggccac gaggatcctg ccggagctcg
     8761 aggcggcagg tgacgtgacg acccatgaca gctccacgaa cgggctgatc aatttgttca
     8821 agcagcgccg cggttaacgc gttcgcgaaa atcgcaggga cactttggcc gggcggcggt
     8881 gttacaccgt cgtccgctag ctaccgtgcc tgtcgattcg cagagttact acaccaccta
     8941 cggcccgatg gtgctccggc gttgccggaa gctcctcggg gacgagcacg ctgcacgtga
     9001 tgccatgcac gacgtcttca tccaggtctt gagtcgttcc gaggagcttg tcgaccaggc
     9061 gccctcgtcg ctgctcttca ggatcgcgac gaatgtctgc ctcaaccgca tccgctcgcg
     9121 caaacgtcgt cccgaggatg gcgatccgga cctgctcgca gagatcgcga cgcagaccga
     9181 cccagccgcg cgaacggcgg cccgctcggc gctcgacgcg ctgtttcgcc aagaacccga
     9241 cgacaccgcg ctcatcgcgg tgcttcacct ccacgacaac atgacgctcg aggaggtcgc
     9301 ggcagaggtc ggcatgtccg tatcgggcgt gcgcaaacgc ctcgcaaagc tccgcacgaa
     9361 actccacagc ttggaggtcc aatgagtgct cttcccgatt ggctcgtcga acgagccgcc
     9421 ctcgacgagg tcccggccgc gagtcgcgac cgcctcgagc gcgcggatca acgcgagctc
     9481 caacaacgca tcgccggact gcgcgccgag aacgaggccg agatggccgc gtatcccgct
     9541 gctcaggcca ttctcctcat cgagagccgc gctgctgatg aatcgaagcg gcgcgctgca
     9601 cgtcgacgcc aggtgcaact gcgctgggtc ggcatcctcg gtaccgcgat ggccgcggcg
     9661 gcagtcttgc tggtgctcgg caaacacgac gtcagccatc caccgaccga ggcgccggtc
     9721 gcggcaccag agggcgaccc gatcgatatc acgcgcgcga aaggcccgac gcggttgctc
     9781 gcgttccgcc aggtcggtga ccgcgccgaa cagctcgtcc aagactcggt ggtgcgcgcg
     9841 ggtgatctga tccagctgag ctacaacgcg ggtggcaaac cctacggtgt gatcgcgtcg
     9901 atcgacggcg cgggcgtcgt gacgctgcac ttccctgcga gcgaggatgc gcggccggag
     9961 ggcaccgcgt tggcgaagcg gccaacagcg ctgccgaacg cctacgtact cgacgatgcg
    10021 ccccgcttcg agcgcttctt cttcatcacc gatgattcac ccatcgacgt gtccgatacc
    10081 ctcgccgcgc tgcgcacgtt cgccgcacgc accgacagcg ccaccgcaaa acccgacttg
    10141 ccgccgggcc gaggcggatg ggctttgcgc ttgcgtaaac ccgatcattc gtcgcccact
    10201 cgtgaggaac gccatgacta gccgcctgtt catcgtctgc tctgctgcgc tcgtggccgt
    10261 gctggcgggc gtctccccca gctacgcgaa ggacacggtc gagccgcgac tgcgccgtta
    10321 tgcgctcgtg atcggcagca acaccggcgg cggcgagggg cgcgataagc tgcgctacgc
    10381 cgaccacgac gccgacacgg tcgctgacgt tctgaagcag ctcggcggcg tgaacaacgg
    10441 cgacctcgca cagctcagcg agccaaaccg cgacgcgctc gatcgcgagc tcgacacgct
    10501 ctcgaagcgc gtgcgcgatg aacgcaagcg cggccagcgc gtcgagctcg tcgtctatta
    10561 ctcgggccac tccgacgaga ccgggctatt gatcggcggc gcgcggtacg actacgcgcg
    10621 gttgcgcgat cgcattcgcg cagtccccgc cgacgtcaag atcgcgatcg tcgatagctg
    10681 cgcatcgggc agcttcacgc gcgcgaaggg tggcacgaag gtcccggcgt tcctccacga
    10741 cacgtcgaac caggtcgagg gattcgcgtt cttgtcgtcg agctcggaga acgaggacgc
    10801 gcaggagtct gatcggatca aggcgtcgtt cttcacgtac ttcctcgtca gcgggctacg
    10861 cggcgccgcc gatcgcaacc gcgacggtgt gatcacgctg agcgaggcgt accagttctc
    10921 ctacgagcac acgctcggcc gcacgcagaa cacgagccac ggcccccagc accccgccta
    10981 cgacatgcac ctgtcgggga ccggcgacgt cgtgatcacg gatctgcgca cgtcggactc
    11041 ggcgctcgtg ctgcccgccg atctcaaggg ccacgtcacg atcgtcgacg agaacggccg
    11101 cgtcaccctc gagctgacga aggacgccgg cgagccgctc agcctcgcgc tgccgaagga
    11161 cagctacacg gtccgcgtcg acaacagcaa cggctcgttc gaggcgacga tcacgctcac
    11221 tggcacgcgc tcgaccgagc tcgacaagga cgcactgcgc cgcgtcgcgc ccgagaagac
    11281 cgtggcgcgc ggcctgtcga tcgacgagca cgccgacgat gacgacgacg atgatgacca
    11341 tcacgatccg cggcatcgca accttccgat cagcttcggc ggcggtctgc gtgtcgagct
    11401 gccatccacg catcaggact tcgacgcgct cctctcgatc ggtggcacct cgcgcgaagt
    11461 tcacggccat ccggtgatcg gcgtcgcgga aagcggcgat ctctcgatcg gcatgacgct
    11521 cggcgacaac gtgcacttcg cgtacgacat gagcctcgcg gtcgggcccg gcgtcttcat
    11581 cggtgacaac ctgcagatcg gcacgaccgt cggcttcggc ttctccggca tcaccggcgg
    11641 cgtgctcgac ttcgcgtgga agattccgac cgaagcgttt gtcgtgctcg agctgtcgaa
    11701 ggacgtgcgc ccgatggcgt acttccgcca gaactatcta ttcagctcgg aggcgcggca
    11761 gaacggctcg aagatggcac gctggggcga cgagtcagag gtgggcgcgg gcctgcactt
    11821 ctcgggcgcg ctcgacggct tcctctatgg cagcgtgcgc gagatggcga acgagcgcta
    11881 ctggggcctc ggcatgggcg cgattctgta acggtcacgg agaggcaagt gcggcgagct
    11941 cggtgagtaa aggcgtcacc ttgcgctcgt agaacttgtt accggcgacg gcagcttcga
    12001 gctcacgctt cgccgtcgcg atggcagccg cccggcgctt ctcggcatcg gcgccgtgcg
    12061 cgttcttcaa gctgcgcgcc tcgatggttg caatctcgcc gacgtagagg tgtgcagccg
    12121 gcgcgtcgtg caccatctcg accgcttgcg acgcggcggc cttcgcgcgc gtgagtgtcg
    12181 ccgatgcatc ctgaccttgc gaaacgagcc atgcggcgcg ccagcgcgcg agtccgatgc
    12241 gcgcgtcgat gacaaagccg gtcatcgcgt ccagctgttc ggccttcttc atggcggtgt
    12301 cggcggcagc gaatgcgtcg gcgggtgacg ccttctgtga tatcgcccag cgtgctgcga
    12361 gcacgtggcc gtcggcgcga tacgcgatga tgtccgggtc gggcgtcccc gagcgctcga
    12421 gatcggcgtc gatcgcggtt tcgagcttcg ccaacgcggg ggcgggatcc ttgccgttct
    12481 cgacgaggta ttctgcttcg atcagccgca accccgccat gttgaagtac gagaaccgct
    12541 cggcagggtt cgcggcgagc gcgcgctcga ggtagctgtt gccttcctcg atcactgggc
    12601 gcggatcgcg gccgatgcgt gcgagatacg tcgcgcgatc gatggtgagg tagccgatgt
    12661 tgttgagcac cttcgagtca gtgccaccga gcgcgagcac gcggtcatag gcttctttcg
    12721 cgcgcatgat gtcggcttcg ccctcgactt ggccgtgctc gaaccggtag tacgcgcgcg
    12781 tatgaaacgc gaggccgatg ttgttcaggg ccgtcgcgtt cttcggattg atcgcgagcg
    12841 atgcctcgaa cgcgtcgatg gcatgaccga gcgaggcctg tggatcgatg ccgtgctcgg
    12901 cctcgtagtc agcgcgcgca tgccacacgg tgccaaggtc ggtcttcagc gtgctcgact
    12961 gcctggacgt tgcgatgcca cgttccgcgt acgccaccgc ttcgtcgagg atcggtcgcg
    13021 gatcgtgacc ggcttcgcgc tcgcgctcgg cgcgttgttg caagatgaac gcgaggtcgg
    13081 aatacgcatc ggcgtagctc ggatcgagct ccgtcacctt gtggagcgcg gcgattgcct
    13141 ggtcggtgct ctgctcggga tgctcgcggg cgtcggcctg ggcttgcgag agcgcggcgt
    13201 acaacacgga gcgcacgtag tacgcctcgg cggacctcgg atctttcgcg agtgcggcct
    13261 cgatcagcgg cagcgcgcgc ttcatcgctt catcgggacg cgtgccgcgc cggctggcaa
    13321 ggaagtccgc ggcgtcggca atgatgtacg cctcgcggag catcaccgtc acggcatcgg
    13381 gatcggcacg gcgcgcgcgt tcacagggct cgaccgcttc gtcgaccatg tcctggtcga
    13441 gctggcgctg cgcgattgcg aggctcacct tcttgccgcg ccgcttgcac tcggcatcga
    13501 gcagctgtgc gtcacttggc gctgcggcca gcgcttgcgc gtactcttcg ccggcacgct
    13561 cgaggtcggc ggcggcttcc gcatagtttc ccgtgtgact cgtctcgctc gaccgcgcgg
    13621 tgaacgcgtc tcccgcgagc ttgtgcgctt cgtagagcca cggcgcggct tgcgacgcag
    13681 cggccgcctt tgtgagcgcc gtcgcgaagt caccttcgta aaacgcgacg agagcctcgg
    13741 cgtatgcagg tgcttcgggc gcggagtcac cgaccgcacg tagatacgcg agtgccggat
    13801 cgcgcagcgt cttctttagc tcggcgcgct ttgcgtcgcg cacggcgctg tcgcgaacgc
    13861 gctcggcgtc gtacacacct tcctgataca gcgctgcgag cgtgagcccg agcgcgtaac
    13921 gcgcttctgg cgggtcgtag ccggcggcga gcgccgcgtc gaggtgtggc ttcgcctgct
    13981 tgtagtcatg caaggagaga tggccgcggc cgaccgcgta ctcggcggga cccgtcgcga
    14041 tgtcgcccag ctcgcgtgct tccgcttgga tcgcagtcat tcgatcgaga acgtgtttac
    14101 gctcgggccg cgtatcgtga agcggcgccg cataggcttg ccgcatgaac gtttcgagcc
    14161 gcgcgacctc ctggccgaac cgttgcgcga tctgcacgcg cttctcggcg agccggcgtt
    14221 ggtgcgcggc attgagcacc agcgcgacga ccgcgacgat cgcgatcgcc gccaccgcga
    14281 gcgcgatggt gagcccccac gagcgtcgca gccgcttgct cgcgcggtac atcaggctgc
    14341 gccgccgcgc ggcgatcggt tcgcccgcga tgtagcgccg gatgtcattg ccgagctcgg
    14401 ctgcggtcgc gtagcgccgc tccggcttct gctcgaggca cttcatcacg atcgtcgcga
    14461 gctcgcttgg aatctgcggc gcgactcggc ggatcgacgg cacctcctcg gtgacaagcg
    14521 acttgaatgc ttcgggcgtc gtgccttggt tcggtgggcg gccggtcaag agcgcgtaga
    14581 acgtcgcgcc gagaccccag acgtccgtgc gtttatcggc gcgttcgccg cgcgcctgtt
    14641 cgggcgccat gtatgcgggc gtgcctgcga cttctccggc aagtgtcgcg tcggcgtcga
    14701 cttcgcgtgc gagcccgaag tcgagcacga acggttgcca cttaccgctg tcggtgcgct
    14761 ctaccatgat gttcgcgcgc ttgaggtcgc gatggatcag accaccttga tgcgcggcgt
    14821 ggacggcgtc ggccaccttg cgcatcgtgt ggaccttgtc ctccgtcgtc atcttttcgc
    14881 cggcgtgacc gagcgtgacg ccgtcgatga actgcatcgc gatgtacggg atgccttgcg
    14941 cagtgccgac ctcgtagacc ttgcagacgt gctcgtgctc gacacgcgct tgcgcacgcg
    15001 cctcggcaat gaaccgtcgc gcgcgctcgg gctcggaccg cgtgaggaac ttgagtgcga
    15061 cgaagcggcg gagctgggga tcgcgggcct tgtacaccgt gcccatgccg ccgcggccga
    15121 tgtagctgat gagctcgtaa cgatcccagt tcgcgatcgg gaacccgacc ggtgtcggtc
    15181 gccgcggcgc atcggggatg ccgtcgccgg tttgtgtgct cagcgtcgcg tgcggcaact
    15241 ccgggtcggc gccgaagaac gattcgcgcg gcgtcgtgac ctcgtcgatg ctctgcgatg
    15301 gctgcgacgg ctgcgacggt cgctcgcgaa ccgtctcgtc gacctccgag ctcgcgttgc
    15361 cttcccgatc cgccgacatc aggccaacga gcaggatctc acccgacgga aatcgggtca
    15421 acgggcgtcg acgacccgga tccctcggga atcagggact cccggacaac cagaccgtca
    15481 gttcgcgttg aagtacgcgg caacgtggac gccggtcggc ggatcgcatt tcgtccagat
    15541 gccgagaatc ttcttgcaga cgacgtcgat cttgccggtg aacagcgcgc cgccctgcac
    15601 cgcggtcatc gccgcgctcg ggttcgtcgc cgcgctgtag tcgtaggtga ccgacgaggc
    15661 gctcggatac gtgcgctcgt tgacgagcgt gccggtggct ttgttgccgc tcagcgagag
    15721 ctgatacagc gcatcgcgct gcgcgacgcc ggtcaggcct tggccatcga cgatcgcaac
    15781 cggctggctc ggcgtgtaga gctcgaccac atcgacgcgc gggttctccc acggcttcga
    15841 gttgttgcgg ccaccgatga tcgcgatcac gcggttctcg ttgcccgcgt acgggatcgc
    15901 aatgaccgca tcgagcgcgg cctgcgtggt cagcgcgtca tccgcgagcc gaatctcggg
    15961 caacgtcgca gcgccgtacg tcgggctgag cgtgctcggc ttcgcgttcg cgcggcgcag
    16021 tgcgagcggg ccgaagccct gcgaggtcgg cgatagtgga gacacgtaga agtagagaag
    16081 tcccgaggtg acctcggact cgtccgcctt cgtgtcgcct ccgctggtgc tggtgtactg
    16141 gtcagcgtcg tccgttgctt ccgcacaacc agcaacgccg accacgacgc aaacgaacaa
    16201 ggagcagtaa cgcatggcgc ctcataccta gcgatgcagg gagaggtcca aacatgaatt
    16261 tcaacggatg ctcacaggag gtgacaccgt gacgtcgcgt acgtatgttg caaccgctct
    16321 acgcagactc tgcgcgagca aggtaaataa gcgccgtgcg cgaggattcc aagctgctca
    16381 aggccgtctc ggctctgacc tcgcacgtcg atggaatcga ctgggatgca gctgcggtgc
    16441 aggcggatgt cgatcggatg ctcgaggtcg tcaagcaggc gtcggcatcg gcgcgtgcag
    16501 aagcgctcgc ggttctggtc gagcggttgc ggcgcgcgcg gctcgacgat gctgacggcg
    16561 tcgcgcacgc cgcgatcagt gcgggcacgc tcgtcgagta tggtgcgcct gccgagccac
    16621 tcgcgaacgt gctgctcgac aagctgccgg ccgtgctcgc agcggcgcgt cgctacgccg
    16681 acgcatgcct cgccgacctg ccgccgctcg gtgacaacga agaagacgac ccggcgagcg
    16741 cactcgtggt gatcgatggg cgcggcatca cgctcgatgt atttcgttcg cacctcgacg
    16801 cggatcgagc gggtgcaggc gccctacatg gcttgaatca gtgggcgctg gccgcgatcg
    16861 ccagcctgac gcgctcgcgt gccgcactcg ttcgcgcgtg tgccaacgat gagctacgca
    16921 ggctcgcccg cgcgatggcg gactcggacg caatgtggct cgacacactc ttcggcgtcg
    16981 agctcgacgc gcgctggctc gtgctctgcc cactcgtcga gcgcggcttt cggatcagcg
    17041 tcgacggcat cgcatcgaac ttcgagctcc acacgctggt cgccgacacc ctcatcgcgc
    17101 aagg
//
LOCUS       DASUFX010000050         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1536320, whole genome shotgun sequence.
ACCESSION   DASUFX010000050 DASUFX010000000
VERSION     DASUFX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536320"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1015
                     /locus_tag="VFV99_01895"
     CDS             200..1015
                     /locus_tag="VFV99_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX5058076.1"
                     /translation="MAYRLLAVDLDGTLLTSDKRVDERDAAAIHELRRGGVTVTINTG
                     RLHSGSIEAARVCGIVGPIACVEGSHVVEAESERTLVRHPMTPAVTHLLREAFRGLAT
                     FVFEHDGIHHDQDGEAYAHYVATWSPKLRLVEEALDEHVWANDPLAAVAIGDAQTVGA
                     AHAVLRAHAADVFSVSFPIWQYPGKHAILVRAAGPNKGTALRELCASIGCTVDEAVVV
                     GDWYNDVPMFEVAGRSFVMGGTPDDVRTKATDVLERETGGGGGIAEAIRRAWG"
     gene            1023..2531
                     /gene="ligD"
                     /locus_tag="VFV99_01900"
     CDS             1023..2531
                     /gene="ligD"
                     /locus_tag="VFV99_01900"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5058077.1"
                     /translation="MADTRGRRRRRRTSGENAFTFARQLTLTPIAPGDARTAAGDLIE
                     PAWDGHRVLATRVGDDVRIAALDFRDWTQTFPAAARALGKLAASKVAIDGVVCVLDPR
                     GAPSFEQLRVAVAKGAAVTDAVVICWDLLWHDDEDLRTKPLEQRRKRLELLLHDAPKS
                     LMPSQALEGTLARVLEAAAPLGLRGVVARAHGGEYTAPWCAYSTSATAIDWRRSLSPP
                     PPLSNADKVLYPRDGICKRDIVGYYRDVAPLLLPHLIDRPVVVQRWPDGIDEFDWYQH
                     RVPPRAPDYLRAAWVDGVRRIVIENEDALLWMVNQAGLTYHGFASRLSSIAEPDYAVI
                     DLDPGDKTTWPDVVEIALAVRRVLELLELPNVLKTSGQRGLHILIPLAPGQTFAQAEE
                     LGRGVASLMLRLLPDKCTVENEKEKRGGRLLVDHKQFMAKTLVVPYSLRAVDRATAST
                     PLAWDDLTPALDVRSFTLRTLRARLDKHGDLAAPLLRAGSQLAPALAQLRRQ"
     gene            complement(2532..3086)
                     /locus_tag="VFV99_01905"
     CDS             complement(2532..3086)
                     /locus_tag="VFV99_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058078.1"
                     /translation="MRLFVVTLLALCACSKHEPPPPPPEKPAEPPAPRKKIGPADFGS
                     CTLKATGGFTGEETIPGDQKSARSKYWQSEDERGATPLPALTVNCSGKDMRVSIVSAP
                     HADVPYGLKTYDVEGKNSEVVLLGRAGDQLSDFKGTVVVDAFDNKHIVGSIKISAKVG
                     MSKKRVNIEGNFDFACPGMAGCAR"
     gene            3107..4309
                     /locus_tag="VFV99_01910"
     CDS             3107..4309
                     /locus_tag="VFV99_01910"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX5058079.1"
                     /translation="MTVSDLLDVLFRWTHLIAGIMWIGNSMLFNWLDRNLEKQANLSR
                     LSQGKIFMLHSGAFYDVEKKLLEPGEMPETLHWFKWQNFTTWASGISLLIVIYYMGKV
                     PLVDPSIKDISNGVAVTLSVCVLFTAWLIYDGLWRTLGEKNPRLATGISIALLFGSIF
                     AFTRIFSGPAAYMQTGVMIGTLMTGNVWMVIVPSQHALVDATKTGKEQDASLSIRAKQ
                     RSIHNNYLTFPLLFIMLSNHFPAATGHPLNWAVLIAVMIGGAGVRHFMNIRYRGGGKQ
                     LTKAAWLAPAFLMGMIGVSGLFVFTHIKVHTGPKVTGPVPFARAQEIIVQRCMRCHSS
                     HPTDEIYKVAPNNIMFDTPDQIRLMKDRIYERAVNQRTMPLVNKTKITDMERAELGKW
                     IDDGAKLE"
     gene            4367..4621
                     /locus_tag="VFV99_01915"
     CDS             4367..4621
                     /locus_tag="VFV99_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058080.1"
                     /translation="MEILMISPEEPKETPPEPVHDPDQEPEEPPHKQPPPPEVPERKE
                     PPEREPVRRYPPTHPPDPRDPTPHKIDEPPEPGAPPEIIA"
BASE COUNT          766 a         1579 c         1550 g          782 t
ORIGIN      
        1 ccggttcgcg atcgtggttt cgctgacctt gacgtcaggc cacgcagcgc ggccggcggc
       61 ggcgatgcgc gaggctgcag cggctacgtc cggcgacacg gccaccatcc tagtatccag
      121 gcgggtcgca tgccaccgcc gagtatcccg gggttccggg gggctggccc ccggatgtgg
      181 ggtggtagaa tgggccaaca tggcgtatcg gctgttggct gttgacctcg atggcacgct
      241 cctgacgagc gacaaacggg tcgacgagcg tgacgccgcc gccatccacg agctgcgccg
      301 cggcggcgta accgtcacga tcaacacggg tcggctccac tcgggctcga tcgaggctgc
      361 acgagtgtgc gggattgtcg ggcccatcgc gtgcgtggag ggcagccacg tggtcgaggc
      421 cgagtccgag cgcacgctgg tccgccaccc gatgaccccg gccgtcacgc acctgttgcg
      481 cgaggcgttc cgcggcctgg cgacgttcgt gttcgagcac gacggcatcc accacgacca
      541 ggacggcgag gcatacgcgc actacgtcgc gacgtggtcg ccgaagctgc ggctggtcga
      601 ggaagcgctc gacgagcacg tatgggcgaa cgacccgctc gccgcggtcg cgatcggcga
      661 tgcgcagacc gtcggtgccg cgcacgccgt actgcgcgcg catgccgccg acgtgttctc
      721 ggtcagcttc ccgatctggc agtacccggg caagcacgcg atcctcgtgc gcgccgccgg
      781 cccgaacaag ggcaccgcgc tccgcgagct gtgcgcgtcg atcggctgca ctgtcgacga
      841 agcggtggtc gtcggcgatt ggtacaacga cgtgccgatg ttcgaggttg ccggccgctc
      901 gttcgtgatg ggcggtacgc ccgacgacgt gcgaacgaag gcgaccgacg tgctcgagcg
      961 cgagacgggt ggcgggggcg gcatcgccga ggcgatccgt cgggcgtggg gctgacgctc
     1021 gcgtggcgga cacgcgtggc cgccgccgtc gtcgtcgcac gagtggcgag aatgcgttca
     1081 cgtttgcccg ccagctgacg ctcacgccga ttgctcccgg tgatgcgcgc accgccgcgg
     1141 gcgacctgat cgagccggcg tgggatggcc atcgcgtgct cgcgacgcgc gtcggcgatg
     1201 acgtgcggat cgcggcgctc gactttcgcg actggacgca gacgtttccg gccgccgcac
     1261 gcgcgctcgg caagctcgcc gcgagcaagg tcgcgatcga cggcgtcgtg tgcgtgctcg
     1321 atccgcgcgg tgcgccgtcg ttcgagcagc tgcgcgttgc ggtcgccaag ggcgcggccg
     1381 tcaccgacgc ggtcgtgatc tgctgggacc tgctctggca cgacgacgag gacctacgca
     1441 ccaagccgct cgagcagcgg cgcaagcggc tcgagctcct gctccacgac gcgccgaagt
     1501 cgctcatgcc gtcgcaagcg ctggagggca cgctcgcgcg cgtgctcgag gcagcggcgc
     1561 cactcggtct acgcggcgtg gtcgcgcgtg cgcatggcgg cgagtacacc gcgccgtggt
     1621 gcgcgtactc gacgagcgcg actgcgatcg actggcggcg cagtctgtcg ccgccgccgc
     1681 cgctctcgaa cgccgacaag gtgctctatc cgcgcgacgg catctgcaag cgcgacatcg
     1741 tcggctacta ccgcgatgtc gcgccgctgc tgctgccgca tctcatcgat cgcccggtcg
     1801 tcgtccagcg ctggcccgac ggcatcgacg agttcgactg gtatcagcat cgcgtgccgc
     1861 cgcgcgcgcc cgactatctg cgcgcggcgt gggtcgacgg cgtgcgccgc atcgtgatcg
     1921 agaacgaaga cgcattgctg tggatggtga accaggcggg cctcacctat cacggcttcg
     1981 cgagccggct gtcgtcgatc gccgagcccg attacgcggt catcgatctc gatccgggtg
     2041 acaagacgac ctggccggac gtcgtcgaga tcgcgctcgc ggtgcgtcgt gtgctcgagc
     2101 tactcgaatt gccgaacgtg ctgaagacga gcggccagcg cggcctccac atcctcattc
     2161 cgctcgcacc cggtcagacg ttcgcccaag ccgaggagct cggccgtggc gtcgcgtcgc
     2221 tgatgctgcg tctgctcccc gacaagtgca ccgtcgagaa cgagaaagag aagcgcggcg
     2281 gtcggttgct cgtcgatcac aagcaattca tggcgaagac gctcgtcgtg ccgtactcgc
     2341 tgcgcgcggt cgatcgcgcc acggcgtcga cgccactcgc ttgggacgac ctgacgccgg
     2401 cgctcgacgt ccgctcgttc acactgcgca cgctgcgcgc ccgcctcgac aagcacggcg
     2461 acctcgcagc gccgctcttg cgcgccggta gccagctcgc gcccgcactc gcgcagctgc
     2521 gccggcagtg actagcgcgc gcagccggcc atgccggggc acgcgaaatc gaagttgccc
     2581 tcgatgttca cgcgcttctt gctcatgccg accttcgcgc tgatcttgat cgagccgacg
     2641 atgtgcttgt tgtcgaacgc gtcgaccacg acggtgccct tgaaatcgga gagctggtca
     2701 ccagcgcggc cgagcagcac gacctcgctg ttcttgccct cgacgtcgta ggtcttgagg
     2761 ccgtacggca cgtcggcgtg cggcgccgac acgatcgata cgcgcatgtc cttaccgctg
     2821 cagttcacgg tcagcgccgg cagcggcgtg gcgccgcgtt cgtcctcgga ctgccagtac
     2881 ttgcttcgtg ccgacttctg gtcgcccggg atggtctcct cgccggtgaa gccgccggtc
     2941 gctttcagcg tgcagctgcc gaagtcggcc gggccgatct ttttgcgcgg ggcaggcggc
     3001 tcggccggtt tctcaggcgg cgggggcgga ggctcatgct tgctacaggc gcacagagct
     3061 agcagcgtga cgacaaacaa ccgcatgtgc cgactatact cgcgacgtga ctgttagcga
     3121 tttgctcgat gtgctgttcc ggtggacgca cctgatcgcc ggaatcatgt ggatcggcaa
     3181 ctccatgttg ttcaactggc tcgatcgcaa cctggagaag caagcgaatc tatcgcgcct
     3241 ctcgcagggc aagatcttca tgctccactc gggggcgttt tacgacgtcg agaagaagct
     3301 gctcgagccc ggcgagatgc cggagacgct gcactggttc aagtggcaaa acttcacgac
     3361 gtgggcgagc ggcatctcgc tgctcatcgt catctactac atgggcaagg tcccgctcgt
     3421 cgatccatcg atcaaggaca tttcgaacgg cgtcgcggtc acactcagcg tgtgcgtgct
     3481 gttcaccgcg tggctgatct acgacggcct gtggcgcacg ctcggcgaga agaacccgcg
     3541 actggcgacc ggcatttcga tcgcgctctt gttcggctcg atcttcgcgt tcacgcggat
     3601 cttcagcggg cccgccgcgt acatgcagac cggcgtcatg atcggcacgc tgatgaccgg
     3661 caacgtgtgg atggtcatcg tcccgtcgca acacgcactc gtcgacgcga cgaagaccgg
     3721 caaggaacaa gacgcgtcgc tgtcgatccg cgcgaagcag cgcagcatcc acaacaacta
     3781 cctcacgttc ccgctgctgt tcatcatgct gtcgaatcac ttcccggcag cgacggggca
     3841 tccgctgaac tgggcggtgc tgatcgcggt gatgatcggc ggcgcgggtg ttcgccactt
     3901 catgaacatc cgctaccgcg gcggcggcaa gcagctcacg aaggcagcgt ggctcgcgcc
     3961 cgcgttcctg atgggcatga tcggcgtctc cgggttattc gtgttcacgc acatcaaagt
     4021 gcataccggg ccgaaggtca ccgggcccgt gccgttcgcg cgtgcccagg agatcatcgt
     4081 gcaacgctgc atgcgctgcc attcgagtca tccaaccgac gaaatctaca aggtggcgcc
     4141 gaacaacatc atgttcgaca cgcctgatca gattcgcctc atgaaagatc gaatctacga
     4201 acgcgcggtc aaccagcgca cgatgccgct cgtcaacaag acgaagatca cggacatgga
     4261 gcgcgccgag ctcggcaagt ggatcgacga cggcgccaag ctcgagtagc gtggcagcca
     4321 cgcacgtccc tacgtacttg gacggctgat tgcacttctt cccttcatgg aaattctgat
     4381 gatttcgccc gaggaaccga aggagacgcc gcccgagccc gtgcacgatc cggatcagga
     4441 gcccgaggag ccgccgcaca agcagccgcc accgcctgaa gtgcccgagc gcaaggaacc
     4501 accggagcgc gaaccggtgc gccgctaccc gccgacccac ccacccgatc cgcgcgatcc
     4561 gacaccgcac aagatcgacg aaccgccgga gcccggcgct ccgcccgaga tcatcgcgta
     4621 gctaacgggg tcaggcacta ctggttctgg ttacgcgatt cgcatctctc gcgctgc
//
LOCUS       DASUFX010000051         5095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_285809,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000051 DASUFX010000000
VERSION     DASUFX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285809"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..994)
                     /locus_tag="VFV99_01920"
     CDS             complement(308..994)
                     /locus_tag="VFV99_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058081.1"
                     /translation="MSGRGSLAVESTTPVVYQEPPPPQQEVVTVRPGYLWIHGRWDWR
                     NGQWAWVGGHWERERAGQYWTEGRWDNRGNQWVWVEGTWQMGSNNPTPPPTASAQGGV
                     VVTGGTTAPPPVVVAPPPTNTATAQGGVTVTGGGGGTVVISQYPTAAPPPVRVETVQA
                     KAGYVWIAGRWDWQRGQWAWVDGHWERERANQVWVTGSWQLQGNRWVWVEGRWDVRAA
                     PGPQVRDHRH"
     gene            1258..3048
                     /locus_tag="VFV99_01925"
     CDS             1258..3048
                     /locus_tag="VFV99_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058082.1"
                     /translation="MHRLVVVIAIAACGNPKDATPDAFVPPIDAPVAPVFRNPVDLPD
                     DQLATEALKILGANVSGAAQACEGCHGMTRQRLSYWRALSDTAMTSCLTDLSVATQES
                     AQKMIDCTRAMPEIPGSDFATPKLGIYATATQLPWFGFVFWRAYGDEAPTKLAEFQKA
                     IGMPKDNATTPLTQEKFDIVAEWFARGVPALDGALPTDPPPQSCTPGISSEVAAHVSA
                     MATQGWRAVNNSTMMAMFDCGRATDPRQCLADKPLATAQAWGTGWDIAGRGNARLLND
                     VTYVSSYWTRSSPDGRFVAHGVANVQGSYVIDLQRNATVSIATQYDPAFFPDNTGFVF
                     QGGTRNVCAISVLTSNPDSIAMTEAACSKISSIGLYEHVGRALDGGDFFGIDSQFVSD
                     DGGKSPTRSDPDADFTSTGYTDFTPAIFDGTKYVAKSRVRVQQPFEGDSVLSPSAKLE
                     ITRVAGPGSNQIGYVLRKVNATPSGSTYTVTTPEIARYCISGGKPGFSYDERWIAFHH
                     YIQDADATEMGFSGPNDPGFAEYKTKGAANLYLMDLRTGTPVRITNVQPGQYALYPHF
                     RSDGWIYAQVRDTNLGHEYTIATDAALVAE"
     gene            3181..>5095
                     /locus_tag="VFV99_01930"
     CDS             3181..>5095
                     /locus_tag="VFV99_01930"
                     /inference="COORDINATES: protein motif:HMM:NF041916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6230/SCO0854 family RING domain-containing
                     protein"
                     /protein_id="HEX5058083.1"
                     /translation="MDRIRTLLLARTNVVVMDPDLVASASTRPSRDQDAEKLEDELAQ
                     LGYVMSLDLATMVRRLPYQTMQELRNWIYDTLAKQVGAHRPHVPLFRNFPDGVPANTQ
                     ALYLKRMLSWLLTKPDQPCPWCGKLTRVGALDPCGHLVCRSCWDGASFAGCPICHRRI
                     AREPFMKATAGEAVTKHSGALTLLDLGFDLIGIAKDRFKRLLTRVTPLSPDDRAEVEA
                     VIDAVGPKVVTWFPERISVRETLAIAVARLVLISPNRATMMRDLAPYVTSATDVLRIA
                     AVLMGANAELVEPMRFGSLGRALRRALLEALERLPLETVVEDVMRHPGIWKRAGERLH
                     PFEYAEQVPNTCVAFALVRGTNLAKSTLGAMLKHHAVKHQFLYLDNDRIKAVAWAGPI
                     EDALRMANPRSALARLTHRPGELLRRADHLVRVAQIRQPDALQTILKAIELAAVKGPP
                     ATMLTLASHVARRGRNWPRRVFFPKGEVLHAWGMDDNRVPLRGDAIAVIVGSIRRALV
                     HRAESKRQFPRAVIDRGLVDLLLPVNERTTSRARLAWPRGSEIALPPGQTMRLFLHWE
                     EPTGTRVDLDLSCALFDAQWKHVATCDYTNLVVGERAAVHSGDLASAPAPLGASEFVD
                     LHLERLAMMGARHL"
BASE COUNT          852 a         1716 c         1693 g          834 t
ORIGIN      
        1 ggcgtacgtc gccggttatc gcggcctcgg ccttgcgtac gcacagaaag gcgacaaggc
       61 agcagcgacc aaagcgctgc gcacgtactt gaacgcagtg cccggcgcga aggatgccgg
      121 gctcattcgc aagcgcatcc agacgctcaa gtagcggcga cgaagtcgcg ccgacgtcga
      181 cggagtcgcg ccgacgtcga cgggtgcgac tctcgatgtc cgaaacgcaa acagccgact
      241 cgtaatgagc cggctgtttc tggtttcgga tctgacccga ggcctgaagc ctgaggcccg
      301 aagcccgtta gtgtcggtgg tcgcgaacct gcgggcccgg cgccgcgcgc acgtcccagc
      361 ggccttcgac ccacacccag cggttgccct gcagctgcca gctgccggtg acccaaacct
      421 ggttcgcgcg ctcgcgctcc cagtggccat cgacccacgc ccactgaccg cgctgccaat
      481 cccagcgacc ggcgatccag acgtagccgg ccttcgcctg caccgtctcg acgcggaccg
      541 gaggcggcgc cgccgtcggg tactgcgaga tcaccaccgt gccaccacca ccgccggtga
      601 cggtcacgcc accttgcgcg gtcgccgtgt tggtcggcgg cggagcaacg acgaccggcg
      661 gcggagccgt ggtgccaccg gtgacgacga cgccaccctg tgcactcgcg gtcggcggcg
      721 gcgtcgggtt gttcgagccc atctgccacg tgccctcgac ccacacccac tggttgccgc
      781 ggttatccca gcggccttcg gtccagtatt gaccagcgcg ctcgcgctcc cagtgaccac
      841 cgacccacgc ccactggccg ttgcgccagt cccagcgacc gtggatccag aggtagcccg
      901 gacgaaccgt cacgacctct tgttggggcg gcggcggctc ttggtaaacg accggcgtcg
      961 tcgactcgac cgccaggctt ccacggccgc tcaccagaca tgcggccgaa aattgaagcc
     1021 caagcgcgag tacgagtaga agagttcgtg aacgtgtcat ttggttagtc tcccttgtgc
     1081 ggatgggacg gccaggtccc cggatttctt caacggcgga gtattcccaa ggcccctagt
     1141 gggtgtcaac gcgcggggtc ccaaggcctt acacatgtga gtgagaggca tcacccgcga
     1201 agtagccgtt agggctcgga cgtacatgcc tgcaatcagc cagataagga tgccgccatg
     1261 catcgcttgg ttgtggtgat cgcgatcgcg gcttgtggca atccgaagga cgcgacgcca
     1321 gatgcgttcg ttccacccat cgatgcgcca gtcgcgccgg tgtttcgcaa tccggtcgac
     1381 ctgcccgatg atcagctcgc gaccgaggcg ctgaagattc tcggtgcgaa tgtttcgggc
     1441 gccgcgcaag cgtgcgaggg ctgccacggc atgacgcgcc agcggctgag ttactggcgc
     1501 gcgctctccg acaccgcgat gacgagctgc ttgaccgatc tgtcggtcgc gacgcaggag
     1561 tcggcgcaga agatgatcga ctgcacgcgc gcgatgcccg agattccggg ctccgatttc
     1621 gcgacgccga agctcggcat ctacgcgacc gccacgcagc tgccgtggtt tggcttcgtg
     1681 ttctggcgcg cgtacggcga cgaggcaccg acgaagctcg ccgagttcca aaaagcgatc
     1741 ggcatgccga aggacaacgc gacgacgccg ctcacgcagg agaagttcga catcgtcgcc
     1801 gagtggtttg cgcgcggcgt gcccgcgctc gacggggcgt tgccgaccga tccgccgccg
     1861 caatcgtgca cgcccggcat ctcgtcggaa gtggcggcgc acgtcagcgc gatggcgacc
     1921 caaggttggc gcgccgtgaa caacagcacc atgatggcga tgttcgactg cggccgcgcg
     1981 accgatccgc gccagtgcct cgcggacaag ccactcgcga ccgcgcaggc atggggcacg
     2041 ggctgggaca tcgccggccg cggcaacgca cgcctgctca acgatgtcac ctacgtgagc
     2101 tcgtactgga cgcgcagctc gccggacggt cgcttcgtcg cgcatggcgt cgcgaacgtg
     2161 cagggctcgt acgtgatcga tctgcagcgc aacgcgacgg tgtcgatcgc gacgcaatac
     2221 gatccggcgt tcttccccga caacacgggc ttcgtgttcc agggcggcac gcgcaacgtg
     2281 tgtgcgatca gcgtgctgac gtcgaacccg gacagcatcg cgatgaccga ggccgcgtgc
     2341 tcgaagatct cgtcgatcgg cctctacgag cacgtcgggc gcgcgctcga cggcggcgac
     2401 ttcttcggca tcgatagcca gttcgtctcc gacgacggcg gcaagtcgcc gacgcgcagc
     2461 gatcccgacg cggactttac gtcgaccggc tacaccgact tcacgccggc gatcttcgac
     2521 ggcacgaagt acgtcgcgaa gtcgcgcgtg cgcgtgcagc agccgttcga gggcgactcg
     2581 gtgctgtcgc cgtcggcgaa gctcgagatc acgcgcgtcg ccggccccgg ttcgaatcag
     2641 atcggctacg tgctgcgcaa ggtcaacgcg acgccatcgg gctcgacgta cacggtcacg
     2701 acgccggaga tcgcgcgcta ctgcatctcg ggcggcaagc ccggcttctc gtacgacgag
     2761 cgctggatcg cgttccatca ctacatccag gacgcggacg caaccgagat gggcttctcg
     2821 ggtccgaacg atccgggctt cgccgagtac aagacgaagg gcgcggcgaa cctctacctc
     2881 atggatctcc gcacgggcac gccggtgagg atcacgaacg tccagcccgg ccaatacgcg
     2941 ctgtacccgc acttccgttc ggacggctgg atctacgcgc aggtccgcga cacgaaccta
     3001 ggccacgagt acacgatcgc gaccgacgca gcactggtcg ctgagtaacc acctgcgcaa
     3061 tcagtgcggg ctacgagcct gcatccaccg attgcagatc tgataccgtg ggggttgggc
     3121 ggctccggtt gtgcttcctt ctatcgttcg gtagcgcatc cactctccgc ccaccttttc
     3181 atggaccgga tccgcacgct tctgttagcg cgcacgaacg tcgtcgtgat ggatccggat
     3241 ctggtcgcgt ccgcgtcgac gcggccgtcg cgagaccagg atgccgagaa gctcgaagac
     3301 gagctcgcgc agctcggcta cgtgatgagc ctcgacctcg cgacgatggt gcggcgcctg
     3361 ccctaccaga cgatgcagga gctgcggaac tggatctacg acacgcttgc gaagcaggtc
     3421 ggcgcgcatc gcccgcacgt gccgctgttc cgcaacttcc ccgacggcgt gcccgcgaat
     3481 acgcaggcgc tgtacttgaa gcggatgttg agctggctgc tgacgaagcc cgatcaaccg
     3541 tgtccgtggt gcggaaagct cacgagggtc ggcgcgctcg atccgtgtgg tcacctcgtg
     3601 tgtcgatcgt gctgggacgg cgcgagcttc gcgggctgtc cgatctgcca tcgccggatc
     3661 gcgcgcgagc cgttcatgaa agcaaccgct ggcgaggcgg tgacgaagca cagcggcgcg
     3721 ctgacactgc tcgatctcgg tttcgatctg atcggcatcg cgaaggaccg attcaagcgg
     3781 ctgctcacgc gcgtgacgcc gctatcgccc gacgatcgcg ccgaggtcga agcggtgatc
     3841 gatgccgtcg gcccgaaagt ggtgacgtgg tttcccgagc ggatttctgt ccgcgagacg
     3901 ctggcgatcg cggtcgcgcg cctcgtgctg atcagcccga accgggccac gatgatgcgc
     3961 gacctcgcgc cgtacgtgac gtcggcgacc gacgtgctgc gtatcgccgc cgtgctgatg
     4021 ggcgcgaacg ccgagctcgt ggagccgatg cggtttggct cgctcgggcg cgcgttgcgc
     4081 cgtgcgctgc tcgaggcgct cgaacggttg ccgctcgaaa ccgtcgtcga ggatgtgatg
     4141 cggcatcccg gcatctggaa gcgcgccggc gaacggctgc atccgttcga gtacgcggag
     4201 caggtgccta atacgtgcgt cgcgttcgcg ctcgtgcgcg ggacgaacct cgcgaagagc
     4261 acgctcggcg cgatgctcaa gcaccatgcg gtcaagcatc agttcctcta tctcgacaac
     4321 gatcggatca aggcggtcgc ctgggcgggg ccgatcgaag atgcgctgcg gatggcgaat
     4381 ccgcgctcgg cgctagcgcg gctcacgcat cggcccggcg agctgctcag gcgcgccgat
     4441 cacctcgtgc gcgtggcgca gattcgccag cccgacgctc tgcagacgat cctcaaggcg
     4501 atcgagctcg cagcggtaaa gggaccgccg gcgacgatgc taacgctcgc gagccacgtc
     4561 gcgcgccgcg gccgcaactg gccacgccgc gtgttctttc cgaaaggcga agtactgcac
     4621 gcgtggggca tggacgacaa ccgcgtgccg ctgcgcggtg atgcgatcgc cgtcattgtc
     4681 ggctcgattc gtcgcgcgct cgtgcaccgt gcagagagca agcgacagtt tcctcgcgcg
     4741 gtgatcgatc gcgggctcgt cgatctgctg ttgccggtca acgagcgcac gacatcgcgc
     4801 gcgcgactcg cgtggccgcg cggtagcgag atcgcgttgc cgccgggcca gacgatgcgg
     4861 ctgttcctgc actgggagga accgacgggc acgcgcgtcg atctcgatct gtcgtgcgcg
     4921 ctgttcgacg cgcagtggaa acacgtcgcg acctgcgact acacgaacct cgtcgtcggc
     4981 gagcgcgcgg ccgtgcactc gggtgacctg gcaagcgcgc cggccccgct cggtgcgtcg
     5041 gagttcgtcg acctccacct cgagcggctc gcgatgatgg gcgcacgcca tctcg
//
LOCUS       DASUFX010000052         7377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1953733, whole genome shotgun sequence.
ACCESSION   DASUFX010000052 DASUFX010000000
VERSION     DASUFX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953733"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2754
                     /locus_tag="VFV99_01935"
     CDS             <1..2754
                     /locus_tag="VFV99_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058084.1"
                     /translation="ESTSDLLHPYRAEAKGSGVSTISGKVVGTPAYMAPEQARGEIVD
                     ERADVYAIGALLYELLAGKAPHADETPQAVLDRVIAGPPAPLGEVASDAPDELVDIVG
                     KAMARVPTDRYANGSSLAEDLRRFQTGKLVSAHAYTPWQLARKKLAQHRGVVVMAIAS
                     AIALAAVGVESFRSVVAERDIARGERARAEEARASAEQRKRDLVLVQAETSLRKDPTA
                     ALAWLKLHPVTEDDRMHVIDVMDEALALGAARHVFRPGDWSLDVAFTPDGKTLFAAVR
                     DGRLRAYDVASGKVRELGKSLGPIEAVAVSPDGNWVVTGGGMGDLIAWPLHDGARKIL
                     TERGRPVMQVTFNSDSQRVLVDRDGIAEIVGLDGVVTKLGPESIVRSAVASADWSRRI
                     GKIAPNQVAVLGENGTRLVAQTSNSIGFIALSPKGDTVVVQDSEGVWTVPFTGGTLTK
                     LASYDNKLNEVVWSPDGKKVALVGWLHYIALVDLATREVKQLRGHTDAIYNAQFTRDS
                     TTLLSAGDDGTARVWNVADGSSVALRGHDDDVYRARFSPDERTVATASLDGSVRVWPV
                     DRSGARVLSEGSDILEMDVTGDRAQVTTKTGVARWNLSTGQRDQLFAKQGLQIGLVSP
                     DGESLATPGLGWTLEVRRRTGPSLVLRGHKDFITHAEWSRDSKTLYTSSLDGTLRKWD
                     LTTGASTALVEGDVPVRGFAVAGDNRVAAQVGDTAVMLRPDGTATTLGSGAGWCGTWA
                     QFEKVRDRLLMQRCDRGLLLVDGSTAVNLPTEGYPAIRVAVSADGERIAGAMGDRTVR
                     VWDSQGRSVAVLRGHTDLVMDVAFSPDGTQLASVSYDKTIRVWELATGRHRVLRGHLH
                     AVDRVAWRSPTELVTGSYDGTIRMWPVPPTTPPTQTEIAAKLAAATTAVIDANNRATT
                     SGS"
     gene            2891..3148
                     /locus_tag="VFV99_01940"
     CDS             2891..3148
                     /locus_tag="VFV99_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058085.1"
                     /translation="MARAETEGEPTTTIERDALRDLLDLTVEPPSAQPAHPAQHAHTV
                     PRGQVRHRDDADDLDPQLSPLVVIGVLGLLLVVFIAVSQLH"
     gene            3215..3580
                     /locus_tag="VFV99_01945"
     CDS             3215..3580
                     /locus_tag="VFV99_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058086.1"
                     /translation="MVVLVVEDSSTMRQLICMALRKIEGIVLVEAGDGKDALVKLGEI
                     TPDLLVTDLNMPEMDGFALIAELRARPAFAKLPVLVLTTAGALQDQERAKALGVTGYV
                     TKPIRPDEIVNAVRGALGK"
     gene            3582..4295
                     /locus_tag="VFV99_01950"
     CDS             3582..4295
                     /locus_tag="VFV99_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058087.1"
                     /translation="MSDDDRRSDERFAVALRVDYEDADDLIADYTENLSSGGACVAST
                     REVDIGTEVKLALSFPGLVTPIRVEGIVRWCREEMLGIEFVEGIRAKLETFIERIRSR
                     DPEIVKRTLRVLVVEDNPHIAELLRHGLAGKDMDVAFDCKLASDGRAALELLGEHRFD
                     ALIVDIYLPVLDGAGVISAARTKLDMPALPIIAVSGGGDSARRAALAAGANLFIDKPM
                     RLKSLLERIREIMKLDVAP"
     gene            4292..5296
                     /locus_tag="VFV99_01955"
     CDS             4292..5296
                     /locus_tag="VFV99_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058088.1"
                     /translation="MSARRILVVDDSPFTGEAVAWALRGAGFVVDVARDLWDLEREDL
                     QQPDLVLMDVVLQEAFGDDLAMLLRGVRGFECPIVLLSSLPDHLLERRAADAELDGFI
                     SKREGLAGIVARARQVLGTAAVETEEDAAAGTTGRFEVTARQRVRRILHIAATDKNWN
                     ASAIAGEAHALAGDADLAGAVMLAEAARLCRDVAQRLGAGGATPEIKAAVNALGREVP
                     EARINTGILLVVTSGAFYQDEMLPALDAAGFTVVEAASLVELRPKLHATDYALIIIDD
                     ALRVSEPTLLLEVRKASATNIVFLRDAPGEIDGKAVLARTTGAKRIAVDVQRLVARPA
                     "
     gene            5300..6151
                     /locus_tag="VFV99_01960"
     CDS             5300..6151
                     /locus_tag="VFV99_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058089.1"
                     /translation="MAQPHVLVVDDSEAILAFTRAVLSATYAVSTARNGREGLDLCAR
                     LEPDLVLLDLSMPELDGEAVLEELKANPELNAIPVIIISSEQARAEACVARGGATACL
                     VKPLRADELLALVGRTLDAAQLKRTQGSLAVLPLRIGTMNLAIPLADVRHVLLQPATQ
                     PLPGGPSYLTAFFELFGESVCVLDLGARFGVDHAKPVIDRILVVVEYERVKLALCVDG
                     VHDPEAVLPTDIQPVVPDANELAGALRAVVKTSQGAMPVLHPPALLSRGLVRKLADLV
                     RGGTVSS"
     gene            6148..7344
                     /locus_tag="VFV99_01965"
     CDS             6148..7344
                     /locus_tag="VFV99_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX5058090.1"
                     /translation="MSEPKSAADRALDALLAYAAEWSGFQPESVAREAVRRCLERELA
                     NNASIQAVLRRAAARDPELVRVVREAIGVRETYFFRSPEHFELVASRVAGLAAGGVVR
                     AWSAGCSTGEEAWSLAATIVASMGGRVDQTQQMVVGTDIHAPALEQAKSGTYRLNSQR
                     PSAPMMYPCVTTVGNRLVVNDALRTVTSFAIHDLRDPPPGEFELIFCRNVLIYFKREA
                     AREVLARIASALVPGGLAVFGTIDVDAADVPQLVRIGKPELNAFTTRPTVRARKRPTT
                     LDVVASTVIPSRPAAPAVPEQALALHRSALMWIEMGGRGSADKALAELNRAYPDYVPG
                     ILERALAHARRGDTAGAAHWMTEVMKRIENLPDEQMVVGPEELPVAFYRETARTYLER
                     PREEKP"
BASE COUNT         1214 a         2379 c         2556 g         1228 t
ORIGIN      
        1 gagtcgacgt cggacctgtt gcatccctac cgagcggagg cgaagggctc gggtgtttcg
       61 acgatctcgg gcaaagtggt tggcaccccg gcgtacatgg cgccggagca agcgcgcggc
      121 gaaatcgtcg acgaacgcgc agacgtctat gcgatcggcg cgctgctgta cgagctcctc
      181 gccggcaagg ctccacacgc cgacgagacg ccgcaagcgg tactcgatcg cgtgatcgca
      241 ggcccgccgg cgccgctagg tgaggtcgcg agtgatgctc ccgacgagct cgtcgacatc
      301 gtcggcaagg cgatggcgcg cgtaccgacg gatcgctacg cgaacggaag ctcgctcgcc
      361 gaggatctgc gtcgcttcca gaccggcaag ctggtcagcg cgcacgcata cacaccatgg
      421 cagctcgcgc gcaagaagct cgcgcagcat cgcggcgtcg tcgtgatggc gatcgcgagt
      481 gccatcgcgc tcgctgcggt cggcgtcgaa tcgttccgct cggtggtcgc cgaacgcgac
      541 atcgcgcgtg gcgaacgtgc gcgtgcagag gaggcgaggg cgagcgcgga acagcgcaag
      601 cgcgatctcg tcctcgtcca agcagagacg tcgctgcgca aggatccgac ggcagcactc
      661 gcgtggctga agctgcaccc ggtcaccgag gacgatcgca tgcacgtcat cgacgtgatg
      721 gacgaggcgc ttgcgctcgg tgccgcgcgt cacgtgtttc ggcctggcga ttggtcgctc
      781 gacgtcgcgt tcacgcccga cggcaagacg ttgttcgcgg cggtgcgcga cggcaggttg
      841 cgcgcgtacg acgtcgcgag tggcaaggtg cgcgagctcg gcaagagcct gggcccgatc
      901 gaagcggtcg cggtgtcgcc cgacggcaac tgggttgtca ccggcggcgg gatgggcgac
      961 ctgatcgcat ggccgctgca cgacggcgct cgtaagatcc tcaccgagcg cggtcggccc
     1021 gtcatgcagg tcacgttcaa ctccgacagc cagcgtgtgc tcgtcgatcg cgacggcatc
     1081 gcagaaatcg tcgggctcga cggcgtggtg acgaagctcg gtcccgaatc gatcgttcgc
     1141 agtgcggtgg cgagtgccga ctggtcgcgt cgcatcggca agatcgcgcc gaaccaggtc
     1201 gcagtccttg gcgagaacgg cacgcggctc gtcgcgcaga catcgaacag catcggtttc
     1261 atcgcgctgt caccgaaggg cgacaccgtg gtcgtgcagg acagtgaggg cgtgtggacg
     1321 gtgccgttca ccggcggcac gctgacgaag ctcgcgagct acgacaacaa gctcaacgag
     1381 gttgtgtggt cgccggacgg taagaaggtc gcgctcgtcg gctggctcca ttacatcgcg
     1441 ctcgtcgacc tcgcgacgcg tgaagtcaag cagctgcgcg gtcacacgga cgcgatctac
     1501 aacgcacagt tcacgcggga cagcacgact ctgttgtctg cgggcgatga cggcactgcg
     1561 cgtgtgtgga acgtcgctga cggttcgtcg gtcgcgttgc gcggtcacga cgacgacgtg
     1621 tatcgcgcgc gattctcgcc ggacgagcgc acggtcgcga ctgcgagcct cgacggcagc
     1681 gtgcgagtgt ggccggtcga tcgcagcggc gcgcgcgtgc tcagcgaggg ctcggacatc
     1741 ctcgagatgg atgtcactgg cgatcgtgca caggtcacga cgaagaccgg agtcgcccgc
     1801 tggaacctgt cgacggggca gcgcgaccaa ctgttcgcga agcaaggact gcagatcggc
     1861 ctcgtgtcgc ccgatggtga gagcctcgca acgcccggac tcgggtggac cctcgaggtc
     1921 cggcggcgca ccgggccatc gctcgtactg cgcggccaca aggacttcat cacgcacgcc
     1981 gagtggagcc gcgacagcaa gacactgtac acgtcgtcgc tcgacggcac gttgcgcaag
     2041 tgggacctga caaccggcgc gagtaccgcc ctcgtcgaag gcgacgtgcc ggtgcgcggg
     2101 ttcgccgtcg ccggtgacaa ccgggtcgct gcccaggtcg gcgacaccgc ggtcatgctc
     2161 agacccgatg gcacggcgac gacgctcggc agcggcgctg gctggtgcgg tacatgggcg
     2221 cagttcgaga aggtgcgcga tcgactgttg atgcagcgct gcgatcgcgg cctgcttctc
     2281 gtcgacggct cgaccgcggt gaacctgccg accgaaggtt atccagcgat tcgcgtcgcg
     2341 gtctctgccg acggcgaacg catcgcgggt gcgatgggtg atcgcacggt gcgagtctgg
     2401 gactcgcaag gccgcagcgt cgcggtgctg cgcggtcata ccgacctcgt catggacgtc
     2461 gcgttctcgc cagacggcac gcagctcgcc tcggtgagct acgacaagac gattcgcgtg
     2521 tgggagctgg ccactgggcg ccaccgcgtg ttgcgcggcc atctgcacgc ggtcgaccga
     2581 gtcgcgtggc gctcgccaac ggagctcgtc accggttcgt acgacggcac gatccgcatg
     2641 tggccggtgc cgccgaccac gccgccgacg cagaccgaga tcgcagcgaa gctcgcggca
     2701 gcaacgaccg cagtgatcga cgcgaacaac cgcgcgacga cctcgggcag ctaacgtagc
     2761 gtcggtcgtc aacagctccc gcctgtagtc gtacgtcgac gacggtgccg accgagcggt
     2821 cctgccacag ctcgtctggc tcacggaggc ctgtcgttga gcaggcggca atcagcgcta
     2881 cggtgtcctc gtggcgcgcg cagagaccga aggtgagccg acgacaacca tcgagcgcga
     2941 cgcgcttcgc gatttgctcg acctgacggt cgaaccgccg tccgcgcaac ctgcgcaccc
     3001 cgcgcagcac gcgcatacgg tgccgcgtgg ccaggtccgt caccgcgacg atgcggatga
     3061 cctcgatccg cagctatcgc cgctggtcgt gattggcgtg ctggggctgc ttctcgtcgt
     3121 gtttatcgcg gtcagtcagc ttcactaacc ctggatcgtc gctgcgtttt cgagctagca
     3181 ttacccgaat cacgttggtg gaggtgatct gaccatggtc gtgttggtgg tcgaagacag
     3241 ctcgacgatg cgccagctga tctgcatggc gctgcgcaaa atcgaaggca tcgtgctcgt
     3301 tgaagctggc gacggcaaag acgcgctggt gaagctcggc gagatcacgc cggatctgct
     3361 ggtgacggat ctcaacatgc ccgagatgga tggcttcgca ttgatcgccg agctgcgggc
     3421 gcgacccgcg ttcgcgaagc tgccggtcct cgtgctcacc acggctggtg cactgcagga
     3481 tcaagaacgc gcgaaggcgc tcggcgtcac cggatacgtc acgaagccga tccgccccga
     3541 cgagatcgtg aacgcggtgc gcggagcgct cggtaagtga tatgagcgac gacgaccgcc
     3601 gtagcgatga acgcttcgcg gtcgcgttgc gcgtcgatta cgaagacgcc gacgatctga
     3661 ttgccgatta caccgagaat ctgtcgagcg gtggtgcatg cgtcgcgagt actcgcgaag
     3721 tcgacatcgg caccgaagtg aagctcgcgc tctcgtttcc tggcttggtg acgcccatcc
     3781 gcgtggaagg catcgtgcgt tggtgccgcg aggagatgct cggcatcgaa ttcgtcgaag
     3841 gcatccgcgc gaagctcgaa acgttcatcg agcggattcg ctcgcgcgat ccggagatcg
     3901 tcaagcgcac gctgcgcgtg ctcgtcgtcg aggacaaccc tcacatcgcg gagctgctgc
     3961 ggcacggact cgcaggcaag gacatggacg ttgcgttcga ctgcaagctc gcgagcgatg
     4021 gccgcgccgc gctcgagttg ctcggcgagc atcgcttcga cgcgttgatc gtcgatatct
     4081 acctgccggt gctcgatggc gccggcgtga tcagcgcggc gaggacgaag ctcgacatgc
     4141 ccgcgctgcc gatcatcgcg gtgtcaggcg gcggtgattc cgcgcggcga gcggcgctgg
     4201 cggcgggcgc gaatctgttc atcgacaagc caatgcggct gaagagtctg ctcgagagga
     4261 tacgtgagat catgaagctg gacgtggcac cgtgagcgcg cggcgaattc tggtcgtcga
     4321 cgacagcccg tttacgggcg aagccgtcgc gtgggcactg cgcggcgctg gttttgtcgt
     4381 cgatgttgcc cgtgacctct gggatctcga gcgcgaagat ctgcagcagc cagatctggt
     4441 gctgatggac gtcgtgctgc aggaagcatt cggcgacgac ctcgcgatgc tgcttcgcgg
     4501 tgtgcgcggc ttcgagtgtc cgatcgtgct gttgtcgagc ttgcctgatc acctgctcga
     4561 gcgccgcgct gccgacgccg agctcgatgg cttcatttcg aagcgcgaag gtctcgctgg
     4621 cattgtcgcg cgcgctcgcc aagtgctcgg cactgcggcc gtcgaaaccg aggaggacgc
     4681 ggccgctggc acgacgggcc gtttcgaagt gactgcacgc cagcgcgtgc ggcgaatcct
     4741 ccacatcgcc gcgaccgaca agaactggaa cgccagcgcg atcgcaggcg aagcgcacgc
     4801 gcttgccggt gacgccgacc tcgcaggcgc ggtcatgctc gcggaagccg cgcgcctgtg
     4861 ccgcgatgtc gcgcaacggc tgggtgccgg aggcgcaaca ccggagatca aagcagcggt
     4921 caacgcgctc ggacgcgaag tacccgaggc gcgcatcaac accgggattc tgctcgtcgt
     4981 gacgtcgggc gcgttctatc aggacgaaat gctcccggcg ctcgatgctg cgggctttac
     5041 cgtcgtcgag gcggcctcgc tcgtcgagct gcgccccaag ctccacgcga cggactacgc
     5101 gctgatcatc atcgatgatg cgctgcgcgt ttccgaaccg acgctgctgc tcgaggttcg
     5161 caaggcgtcg gcaacgaaca tcgtgttctt gcgcgatgcg cctggtgaga tcgacggcaa
     5221 ggccgtgctc gcacgcacga cgggcgcgaa gcgaatcgcc gtggacgttc agcgcttggt
     5281 cgcgcgaccg gcctgacgaa tggcgcagcc gcacgtcctc gtggtcgatg atagcgaggc
     5341 gatcctcgcg ttcacccgcg cggtgctgtc ggcgacgtac gcggtgtcga ctgcgcgcaa
     5401 cggccgcgaa gggctcgatc tgtgcgcgcg cctcgaaccc gatctggtgt tgctcgatct
     5461 gtcgatgccc gagctcgatg gcgaggcagt gctcgaggag ttgaaagcaa acccggagct
     5521 caacgcgatt ccggtcatca tcatctcgag cgagcaagcg cgggctgaag cgtgtgtcgc
     5581 tcgcggcggc gcgaccgcat gcctcgtcaa gccgctgcgc gcagacgagc tgctcgcact
     5641 cgtcggtcgc acgctcgatg ccgcgcagct gaagcgcacg caaggcagtc tcgcggtgtt
     5701 gccgctgcgc atcggtacga tgaatctcgc gatcccgctc gccgacgtgc gccacgtgct
     5761 gctgcaaccg gcgacgcagc cgctgcccgg cggcccgtcg tatctcaccg cgttcttcga
     5821 gctgttcggc gaatcggtgt gcgtgctcga tctcggcgcg cgcttcggtg tggatcacgc
     5881 gaagccggtg atcgatcgga ttctcgtcgt ggtcgagtac gagcgcgtca agctcgcgct
     5941 gtgcgtcgac ggcgtgcacg accccgaggc ggtattgccg actgacatcc agcccgtcgt
     6001 tcccgacgcg aacgagctcg ccggtgcgct ccgcgccgtg gtcaagacct cgcagggcgc
     6061 gatgcctgtc ctgcatccgc cggcgctgtt gtcacgcggc ctcgttcgca agctcgcgga
     6121 tcttgtgcgc ggcgggacgg tgtcgtcgtg agcgagccca agagcgctgc cgaccgcgcg
     6181 ctcgacgcac tgcttgccta cgcggcggag tggagtggct tccaacccga gagcgtcgcg
     6241 cgcgaagcgg ttcgccgctg cctcgaacgc gagctcgcta acaatgcatc gatccaagcg
     6301 gtgctgcgac gcgcagcggc tcgcgatccc gagctcgtgc gcgttgtccg cgaagcgatc
     6361 ggcgtgcgcg agacgtactt cttccgcagc ccggagcatt tcgagctcgt cgcgtcgcgt
     6421 gtggcgggcc tagcggcggg tggcgtcgtg cgcgcgtgga gcgcgggctg ctcgaccggt
     6481 gaagaggcgt ggtcgctggc tgcgacgatc gtcgcgagca tgggtggccg cgtcgatcag
     6541 acccagcaga tggtcgtcgg caccgacatt cacgcgccgg cactcgaaca agcgaagtcc
     6601 ggcacgtacc ggctcaactc acaacggccg tcggcgccga tgatgtatcc gtgtgtcacg
     6661 accgtcggca accggctcgt cgtcaacgat gcgttgcgca cggtcacgtc gttcgcgatc
     6721 cacgatttgc gcgatccgcc gcccggcgag ttcgagctga tcttctgtcg caacgtgctc
     6781 atctacttca agcgcgaggc cgcgcgcgag gtgctcgcac ggatcgcgtc cgcgctggtt
     6841 cccggtgggc tcgccgtgtt cggcacgatc gatgtcgatg cggcggatgt gccgcagctc
     6901 gtccggatcg gcaagcccga gctcaacgcg ttcacgacgc ggccgacggt ccgcgcgcgc
     6961 aagcgaccga ccacgctcga cgttgtcgcg tcgacggtga ttccgtcgcg accggccgcg
     7021 cccgctgtgc ccgagcaagc gctcgcgctc caccgcagtg cattgatgtg gatcgagatg
     7081 ggtggccgcg gcagcgcgga caaagcgctt gccgagctga atcgcgcgta tcccgactac
     7141 gtgccgggta tcctcgaacg cgcactagcg cacgcacgac gcggcgatac cgcgggcgct
     7201 gcgcattgga tgaccgaggt gatgaagcgc atcgagaacc ttcccgacga gcaaatggtc
     7261 gtcggccccg aagagctgcc ggtcgcgttc tatcgcgaga ctgcgcgcac gtacctcgag
     7321 cgcccgcgtg aggagaagcc gtgagcattc gcccacgcgc acccaaggag ctcgatg
//
LOCUS       DASUFX010000053         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_981009,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000053 DASUFX010000000
VERSION     DASUFX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981009"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..2114)
                     /locus_tag="VFV99_01970"
     CDS             complement(54..2114)
                     /locus_tag="VFV99_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058091.1"
                     /translation="MTSATCLATTVACTGGNAPELSGLSDQVAQVGTEFKLDLNGTDA
                     DGDRLNYKFKAADLMDIDNRAMITVSPSGAGVFRWTPLAADVGQHAFDFIVSDGGNDT
                     TVTINIDVKSAIGQATAPIFRQPLGTGTTIDLGKAQCVDLHVVVEDQDTAQVEITEQE
                     PKIDGATLNQEDGQSATWHWCPTRAQEGDQRYTLVLAADDGENPKTIKNYLIVLRGDG
                     GNTNCPGGAPTVSHTAANQTTRLDLKPTATIADDKGLKDAPLFYYSFTNPGATQVNLS
                     SMTQLSTTKQSGSNTNGTWAPSLPNPVANAAAGTMKDIWYVFVADDNDDLMGTCDHTT
                     TSAVYHMVVTAGGSTTAGVCSACTADSQCGTGNECVYMGSMGDSYCLQGCTGGCPSGY
                     TCSASKIYSVDAAQDYQCVPQSGSCQAPTGACADDVYEENDTRSAASANPAFAADLYD
                     MSSCPSTTNTYGQDDDWMKVVLAADSRVDLEIAGDGASDLDLHLYRSDGTVITASTSY
                     TSDEAINTCLKAATYYVKVNGYGHAKSDYLFLFDKTAESCDVTCVDDSAEDDDTFSQA
                     RVTSYPTHNATMQTICKNDDDWYKVRLYEGEQITMDLTFTQATSANDIDLHLYKDFTD
                     LWPCSVDDPSGCTAAHGQSASANEHAVYTVPTGTCASGCDYYVVVRGFDGATNTYGIN
                     LSVQ"
     gene            complement(2287..>3508)
                     /locus_tag="VFV99_01975"
     CDS             complement(2287..>3508)
                     /locus_tag="VFV99_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058092.1"
                     /translation="ALGACERLVKNETDPDKRAMHLHRVARIFRAGFGDMKRAERALN
                     LALDGAPTNDEALSELVKFYRDAGDMTSVRVHLNRVAGTMRVRAGNDPKDGIAYRVIS
                     RAMAARAAVGVDGSQPIARAAAELATLLGSAGDPERMLLDAPSHIDLAPLLRTEADDV
                     LFPRSVQTELRQLFTLLGDRVAKHVGVDLRAYGVGRGDRLRARDSSVASHAQDVATGL
                     GFGEIDVYVSAKQPYAMSAEPTSPVSLVIGQSIAQLDSRSIRFAAGAALKLAQTHLAI
                     AARLPSDELGVLVVGLLRMFIPDFPAQGLDEGAIAAQSQKLKRLIPTGLASELRPFAF
                     AIDPLTFQRVDLSRDLRVAGLRAGLIAAGSLMAGLNILAAREQTDVPSFLADPVAQGL
                     VSFALSEDHASVAR"
BASE COUNT          524 a         1157 c         1182 g          645 t
ORIGIN      
        1 cgaactcgcg cagccggcga acgcggctac ggtggcgaga agcgacgcgc gcatcactgc
       61 accgacaggt taatgccgta cgtgttggtc gcgccatcga agccgcggac gacgacgtaa
      121 tagtcacagc cgctcgcgca agtccccgtg ggtacggtgt acacggcgtg ctcgttcgcg
      181 ctcgcgctct ggccatgcgc cgcggtgcag cccgacggat cgtccacgct gcacggccac
      241 aggtcggtga agtccttgta caggtggagg tcgatgtcgt tcgcgctcgt tgcctgcgtg
      301 aacgtcaggt ccatcgtgat ctgctcgcct tcgtagaggc ggaccttgta ccagtcgtcg
      361 tcgttcttgc agatcgtctg catcgtcgcg ttgtgcgtcg ggtacgacgt gacgcgcgcc
      421 tggctgaacg tgtcgtcgtc ctcggcgctg tcgtcgacgc acgtgacgtc gcagctctcg
      481 gcggtcttgt cgaacaggaa caagtagtcg ctcttcgcgt ggccgtagcc gttcaccttg
      541 acgtagtacg tcgcggcctt gaggcaggtg ttgatcgctt cgtccgacgt gtagctcgtc
      601 gacgcggtga tcaccgtgcc atccgagcgg tacaggtgga ggtcgaggtc gctcgcgcca
      661 tcgccggcga tctcgaggtc gacgcgcgag tcggcggcga gcacgacctt catccagtca
      721 tcgtcctggc cgtacgtgtt cgtcgtgctc gggcagctcg acatgtcgta gagatccgcc
      781 gcgaacgccg ggttcgccga cgcagcactg cgcgtatcgt tctcttcgta cacgtcgtcg
      841 gcgcatgcgc cggtcggcgc ctggcacgag ccgctctgcg gcacgcactg gtaatcctgg
      901 gccgcgtcga ccgagtagat cttcgacgcc gagcaggtgt agccgctcgg acaaccaccg
      961 gtgcagccct gcaggcagta cgagtcaccc atcgagccca tgtacacgca ctcgttgccg
     1021 gtgccgcact gcgagtccgc agtgcacgcc gagcacacgc ccgcggtcgt gctgccgccc
     1081 gccgtgacga ccatgtgata gaccgccgac gttgtcgtgt gatcgcacgt gcccatgagg
     1141 tcgtcgttgt cgtcggcgac gaacacgtac cagatgtcct tcatcgtgcc ggccgcggcg
     1201 ttcgcgaccg ggttcggcag cgacggcgcc caggtgccgt tcgtgttgct gccgctctgc
     1261 ttcgtggtcg acagctgcgt catcgacgac agattgacct gcgtcgcgcc ggggttcgtg
     1321 aacgagtagt agaacagcgg cgcgtccttg aggcccttgt cgtccgcgat cgtcgcggtc
     1381 ggcttgaggt cgaggcgcgt ggtctggttc gccgccgtgt gcgagaccgt tggcgcgccg
     1441 cccgggcagt tcgtgttacc gccgtcgccg cgcagcacga tcaggtagtt cttgatcgtc
     1501 ttcgggttct cgccgtcatc agccgcgagc accagcgtgt agcgctggtc gccttcctgc
     1561 gcgcgcgtcg ggcaccagtg ccacgtcgcg ctctgaccat cttcctggtt gagcgtcgcg
     1621 ccatcgatct tcggttcttg ctcggtgatc tcgacctgcg ccgtgtcctg gtcttcgacc
     1681 accacgtgga ggtcgacgca ctgtgccttg ccgaggtcga tcgtcgtgcc ggtgccgagc
     1741 ggctggcgga agatcggcgc agtcgcctga ccgatcgcgc tcttcacgtc gatgttgatc
     1801 gtgaccgtcg tgtcgttgcc gccgtcggag acgatgaagt cgaacgcgtg ctgaccgacg
     1861 tccgcggcga gcggcgtcca gcggaacacg ccagcgccac ttggcgagac cgtgatcatc
     1921 gcgcggttgt cgatgtccat caggtcagcc gccttgaact tgtagttcag gcgatcgccg
     1981 tcagcgtcgg tgccgttgag gtcgagcttg aactcggtgc ccacctgggc cacctggtcc
     2041 gagagcccac tcagctcggg agcgttaccg ccggtgcagg ctactgtcgt agccaggcaa
     2101 gtggcgctgg tcatggtcaa aagaccgcgg ctgctggtga gtttagctaa cctcatgacg
     2161 ttcctccgag ctgatccgat ggctgtcgga tgccggggcc actgagcaac ggtcgagcca
     2221 aggcgcactg cgccaacgac acgtgcgatc gccaacttcg ttgcgcgcta aatacgcgtc
     2281 agccggtcag cgggcgacgc tcgcgtggtc ttcgctaagt gcaaaagaca ccagcccctg
     2341 ggctactgga tcggccagga agctcgggac gtcggtttgc tcgcgagccg ccaggatgtt
     2401 cagtccggcc atcagtgatc ccgcggcgat gagccccgcg cgcaggccgg ccacccgaag
     2461 gtcccgagag agatccacgc gctggaacgt cagtgggtcg atggcaaacg cgaacggccg
     2521 cagctcgctc gcgaggccgg tcgggatcag gcgcttgagc ttctggctct gcgcggcgat
     2581 cgcgccttcg tcgagtccct gcgccgggaa gtccgggatg aacattcgca agaggccgac
     2641 cacgagcacg ccaagctcat cggacggcag gcgtgccgcg atcgcgaggt gtgtctgcgc
     2701 gagcttgagt gctgcgcctg ccgcgaaccg gatgctgcgc gagtcgagct gcgcgatcga
     2761 ttggccgatc acgagcgaca ccggactcgt cggctcggcg ctcatcgcgt acggctgctt
     2821 cgccgagacg tagacgtcga tctcgccaaa ccccagcccc gtcgcgacgt cctgggcgtg
     2881 cgacgcgacg ctcgagtcgc gcgcgcgcag gcggtcaccg cggcccacgc cgtaggcgcg
     2941 caggtcgacg ccgacgtgct tggcaacgcg gtcgccgagc aacgtgaaca gctgacgcag
     3001 ctccgtctgc acgctgcgcg ggaacaacac gtcgtcggct tccgtgcgaa gcagcggcgc
     3061 gaggtcgatg tgtgacggcg catcgagcag catgcgctcg ggatcgcccg cgctgccgag
     3121 cagcgtcgcg agctctgcgg ccgcacgtgc gatcggctgc gagccatcga cgccgacggc
     3181 tgcacgcgcg gccatcgcac gcgagatcac gcggtacgcg atgccgtctt tcggatcatt
     3241 gcccgctcgc acgcgcatcg tgccggcgac gcggttgagg tgcacgcgca cgctcgtcat
     3301 gtcgcccgcg tcgcgataga acttcacgag ctcggacaac gcctcgtcgt ttgttggcgc
     3361 accgtcgagg gcgaggttca gcgcacgctc ggcgcgcttc atgtcgccga agcccgccct
     3421 gaagatgcgc gcgacgcgat gcaggtgcat cgcgcgtttg tctggatcgg tctcgttctt
     3481 gacgagccgc tcgcacgcac cgagcgcg
//
LOCUS       DASUFX010000054        13974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1953949, whole genome shotgun sequence.
ACCESSION   DASUFX010000054 DASUFX010000000
VERSION     DASUFX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13974
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953949"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFV99_01980"
     CDS             <1..222
                     /locus_tag="VFV99_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058093.1"
                     /translation="RYHAQIWGLFQTLHSRDRIESTGMGLAIVRKIVEAHGGRAWIDS
                     EEGKGSTFRFTWPKDPPRSRSWDRHMRKR"
     gene            256..465
                     /locus_tag="VFV99_01985"
     CDS             256..465
                     /locus_tag="VFV99_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058094.1"
                     /translation="MPRNTKDSQIREDEVERDDVDAPRVDEDEIEPDDRSQSSSKIDE
                     DEDEDDDNVEDIDLDDLSAMEGPDA"
     gene            complement(482..1477)
                     /locus_tag="VFV99_01990"
     CDS             complement(482..1477)
                     /locus_tag="VFV99_01990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058095.1"
                     /translation="MAVSSVKNTFEERHEERHEGSLRTGDLVSAYRIERLLGTGGMGW
                     VFRARHVIDDRVVALKILREDQLRQERAIDRMMREAAILASVAHGGIPQFYECGLLGD
                     GRPWIAMDLIQGTSLSLRIAEGTLPHDTVIELVANVAGVLAAAHERGVTHRDLKPDNI
                     LLTPTDGTYPVRVIDWGIAHHVAGARYTHHNEAIGTPTYMAPEQARGGTVDGYSDIYG
                     LGIVAYHALTGRPPFVGSSSVEILVQHLNRPVPALAPRCPEAPYGLVELVERMVAKDC
                     GQRPTAIEVQRALQKLLPVPSYLFVATEGTQPCARFDLENEHEPPTVLLRPPIAR"
     gene            1632..2774
                     /locus_tag="VFV99_01995"
     CDS             1632..2774
                     /locus_tag="VFV99_01995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058096.1"
                     /translation="MDELGPDKVVGSYRILREIGRGGMGTVFEARHVVLPRRVALKVM
                     HGELRKHPGMATRVVQEASILEEVRHPGVVRVFDCNILPDHRPWIAMELVEGETLANR
                     VLHEGCLAPYEVATLLAEVADVLAAVHARGVIHRDLKPDNLILTPANRDFPLRVIDWG
                     VARLGPIGRLTLDGLTPGTPIYMSPEQATGRNVAAPCDIYALGVIAYEALTGHPPFDG
                     RTLAEVVSMHLTSEPAPLEEQCRAPAELCELVHRMLDKDQTLRPGAIEVRQLARTLAL
                     ELSTAYESIEVIGADAPRLPKAAVRQRRHVALAIERPTEDALIVDPDALEYGVTEMVP
                     TIRKPRWTPEIPVAAAAEAREAARPAITPRGIRDQVAGEIAATKRR"
     gene            3055..3252
                     /locus_tag="VFV99_02000"
     CDS             3055..3252
                     /locus_tag="VFV99_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058097.1"
                     /translation="MRNLVDAGGERVGHIKDRAIGAKDSVVESGQAALSRISALVKEH
                     PFAALGIAFGVGYVAVRLLRK"
     gene            3264..3626
                     /locus_tag="VFV99_02005"
     CDS             3264..3626
                     /locus_tag="VFV99_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058098.1"
                     /translation="METYESQYQREQDIYTEGRAAGIKRKLGDFGRRIKRIDVRTRIT
                     DHPFAAVGIAMGIGAIVGLARPMPKRGPIAGAMMAIASTIGFRMLREAALIQLGHYAK
                     GFLNKDEPGMQQGVPQGY"
     gene            complement(3623..4237)
                     /locus_tag="VFV99_02010"
     CDS             complement(3623..4237)
                     /locus_tag="VFV99_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058099.1"
                     /translation="MRLSALALVLAASTAHADGFYYSQSYGISSARGEGADVLGKSLQ
                     VRIALGWRFGALTVGPLISGNIAVQRDNAYLGFIGGDPPMGDSDLESYGLDARYHATI
                     RDHLVMYVRGGPRFARAEGALDGYSGFGAGVATGVQLTGRVRALGFLFLPLFFAKKGP
                     LITASVFVDESVDWYSLAAQPRDMPALSMPLVGTSIGIAAGSHF"
     gene            4395..5429
                     /locus_tag="VFV99_02015"
     CDS             4395..5429
                     /locus_tag="VFV99_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058100.1"
                     /translation="MTTRLTYFVLPLLVACSDSNSSSDVLGGSLTVTGTVHDFESGAQ
                     VMGAASVSTTALSPTPKVTVEGAAFTIDGVPENSAFQVLASVPTSHRPTYSQVIEVVS
                     DDVDGVSVKTVSEQFLGTLATGFGVTPSAAKGVLLLHLVDGAGAGKAGVAAANLVLAN
                     AAGATGPKFLDANMAPAPAATASTSSGWAVFFEVPPGTIALGQAATATVTLEMASSPV
                     GSGAVTIADVKVTDGAPPATPTNVSFSQQVFPIFTNRGCVACHTGSGPGKDLGGLMLD
                     GGSNLAYKELTVENPLRANTATPEKSLMLTMPSAESPPDGHPNVTFTGPSDPDYIKIL
                     VWIREGAKSN"
     gene            5438..5806
                     /locus_tag="VFV99_02020"
     CDS             5438..5806
                     /locus_tag="VFV99_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058101.1"
                     /translation="MVRLFGVALVAMTLSFACSDNADDLGVGAQCTGNDQCDADTHQV
                     CLQFKGGYCGLMGCVHDTDCPPDSACIMHTDGINYCFRTCVDKPDCNANRDVDNEANC
                     SSSVTFVDGTMGRKACVPPS"
     gene            5865..6938
                     /locus_tag="VFV99_02025"
     CDS             5865..6938
                     /locus_tag="VFV99_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058102.1"
                     /translation="MRLFCALGLAILIARPAHADDPQAMPERVVVKHVSAKRRAAAVA
                     LAIVPGIVVHGIGAWTVGEKRAAKKLFVGEAVGLGVAGLSGLLVGGSGGNPYTVPAVP
                     IVVAGSGLILQSWIEDIFIAAGGENYRGQPRAVAPWSVELGTSWLHDAYRERALLRGG
                     GHIELFGRVVVGAEALVDAGGDAKIGEADARVRILGAPATGALIEDGSRLTARVGSRW
                     HRDDDDRVTQWTQEIGIDGRLDLDRIDRAFRRTFIEAGAGLGIVHVSYGDVAKEWSSE
                     LIAHFAWGAYLCTRGEATVFYEHSRDGLVGGIPAWRASGFVGSVGATVDVRVHGPWAV
                     RGELDIGNAYLTTLAVAYRGGPQ"
     gene            6935..8017
                     /locus_tag="VFV99_02030"
     CDS             6935..8017
                     /locus_tag="VFV99_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5058103.1"
                     /translation="MKHLELALGVVLLAACTRPAEERATQELPVGSAAITDASVEVGG
                     GLAAVRDLTDHRLELWAQSPVLAINLVVGDTAGGDWTIIVRNTLVDAVLDDGGTIYAR
                     DPDQHPTVGIFHVPLAPGSHVLHVAPPDADTIEPFRVAAMADIQTALPEVDDIFEMIS
                     AVPNARFVIAMGDITERSQTDEYDLFEKQLVTLTIPFYTTLGNHELWADPSRFFDRFG
                     RASFQFDFKGASFTFADSGDAGLDPLVEDWVVDWIAAAKNRTSIFLTHIPPIDPLGVR
                     YGGFRSAEDGRRLLTRLAEANVDLTLYGHIHTYVKFENAGIPAFISGGGGADPMKWDG
                     INRHFLVVDIDPAAGAIANVDVVRVD"
     gene            complement(8018..8584)
                     /locus_tag="VFV99_02035"
     CDS             complement(8018..8584)
                     /locus_tag="VFV99_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HEX5058104.1"
                     /translation="MKKLAILTVMGSMLGAAFVAGVAVGKGAAPAPKFTAAEEVKWDD
                     VGGPKLGTLTGDYKKGPYGGLLKIPAGFTSPLHSHTGTYEAIEVQGTSSHWLKGEDGT
                     KAKKMTPGSYWMMPGKVDHVSACAPGADCIFYVWQKSKFDFVAAKEDKAAGSAAAATP
                     AAAKAPAAGSAAPAKAPAAGSAAAPAKK"
     gene            8724..9866
                     /locus_tag="VFV99_02040"
     CDS             8724..9866
                     /locus_tag="VFV99_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5058105.1"
                     /translation="MLTRESCYVDDDIRSAEGLPAAAFSDREFLARELETVFKTSWLV
                     VPERGDDPRPLDEQVAARGSRVPITVLDRPLFVQRGWDDDTLRAFPNTCTHAWYPLVL
                     GPTRGPTLVCGQHGRRFDCTGRFVSQQGFTGDIPNFPRACDHLQQLAIAAWRRLTFVN
                     MGTPAVPFDELFREVDASLANMPPMPERMSAEIRDVAGNWKQHAWNFLDQFHIGFVHR
                     APGGLADAIDLGSYKTELYDRSVLQWVYAREANDGFDPAWLPARFHDDKKGRRVFALW
                     WFIYPNLALNFYPWGLSVNVYQPVPERPEMTRFVWYQFALDRQKHAERDRRWLSSQVD
                     AEDVDALAQVSRGLRSGFAPRGRFAPDHEQAGHWFHRLVSRDVLST"
     gene            complement(9867..10124)
                     /locus_tag="VFV99_02045"
     CDS             complement(9867..10124)
                     /locus_tag="VFV99_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058106.1"
                     /translation="MPMNSATANDVNRLLGDVDPLVLERILETGATPDEIGEALLVLE
                     DERGFGEEPRPPSSTRVAEIRALLQELAVLDSDIEPDEDEM"
     gene            complement(10236..11396)
                     /locus_tag="VFV99_02050"
     CDS             complement(10236..11396)
                     /locus_tag="VFV99_02050"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HEX5058107.1"
                     /translation="MKNASVLVLAFLATFIAACAAEDGGDDDVGSCDTPQTFYPDADG
                     DGYGDMAKGEEHCEAPAGFIAMGGDCNDTNAAIHPDGREICDAIDNDCDGMIDDADSS
                     VDMTTTGTFYRDADNDGYGDAAMTKKACAKPVGYAASSNDCNDNMAAINPGAKEICDY
                     IDNDCDNLIDIADPNIDPTSTKTFYRDQDHDNFGAGAAMVACNAPSGYVEAGGDCNDS
                     DNASWPGATEICDGADNDCDGGVDGTVAQPNRCTALVGSYAGSYSHLTQEKLGSTVIN
                     EMSCTGTGSAALALNRKPGIQGTFTCVYSGGMTLFSQQQRVTISANVRLDGTVTGTVE
                     HTYNTLDNLKRTYNVTGTQTATGLSLTGTGSWYPNPQSAVPWTVSYTFTASK"
     gene            complement(11437..11910)
                     /locus_tag="VFV99_02055"
     CDS             complement(11437..11910)
                     /locus_tag="VFV99_02055"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5058108.1"
                     /translation="MPKITTFLTYEKDAEGAVEHYASIFKSSKVGKKTFYGEGMPLPK
                     GTVMTIEFELEGIPYVALNGGEHFKFTDAISLAVSVETQQEVDNLTDKLIEGGGEEGP
                     CGWLTDRWGLRWQIQPKILIDLISGPDKVKATKAVEAMMKMKRINIAEIKKAAGL"
     gene            11974..12663
                     /locus_tag="VFV99_02060"
     CDS             11974..12663
                     /locus_tag="VFV99_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058109.1"
                     /translation="MTEPAHPREIAIARSGVLLALLVFAAPASADELPAIRIEAKDPA
                     AAKRAVELSKALRAVAAKKTKKSKPTAKDVDSAVVNAECSIVQPACAAEIGEKLAVTH
                     VMVGQLDKRGARYALTLSLINVRTKQRVRSLREVSTTNDMKRWATTLYARMLDEGTGE
                     VVIVANARRGQVLVDGLAVTELYEGRATLSGVALGTHEVEIRASGFKTFVTDVEVDGR
                     SEQSFLLDPAQ"
     gene            12710..13357
                     /locus_tag="VFV99_02065"
     CDS             12710..13357
                     /locus_tag="VFV99_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5058110.1"
                     /translation="MRSTVHTVLVLLLAACGHVDTAAPSPPATLASAPTSSATKPTSE
                     DEQGRAAAATLVGTTPPEWKAERWMNSPPLTLASLRGSVVLVRWWTAGCPFCAATAPS
                     LRTFNRSYADKGLKVIGLYHHKDDGPFDPKVYEDTAKKYEFTFPVAVDPEWQTLQAWM
                     KDKGGNTVETGFTSVTFVLDKHGVVRHVHPGGQYVAGDPAYAELQAVIERLLAES"
     gene            13375..>13974
                     /locus_tag="VFV99_02070"
     CDS             13375..>13974
                     /locus_tag="VFV99_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058111.1"
                     /translation="MPKPPPTPAGKLLELAVTPSYSPRVDGRTLCIPDGNSQNRRFDI
                     DTGKALAPLPFDRTLWLGTGRDRSVRKITDVLDVVDSKGNVICSYKGIGYAHVTPSPD
                     GSMIAIHRVKEPLRIASATDNQLVEIPDGKIKQSCEVAWSPDGSYVVVYSHTEAIVID
                     IKARKATTRLKLRHVTRCLFVPNHNVFLAGDYKRITIWDP"
BASE COUNT         2451 a         4597 c         4493 g         2433 t
ORIGIN      
        1 cgctatcacg cgcaaatctg gggtttgttt caaacgcttc actcgcgtga ccggatcgag
       61 agcaccggaa tgggcctcgc gatcgttcgc aagatcgtgg aggcgcacgg tgggcgcgca
      121 tggatcgact ctgaagaagg aaagggctct acgtttcggt tcacgtggcc aaaggatccg
      181 ccgcgatcac gcagctggga tcgccacatg cgtaagcgct gatacgtggc accgcccgtg
      241 cacttcgctg ttgtcatgcc gcgtaacacg aaagactcgc aaattcggga agatgaagtc
      301 gagcgcgacg acgtcgatgc gccgcgtgtt gacgaggacg agatcgagcc agacgatcga
      361 tcgcaatcgt cgtcgaagat cgacgaggac gaagacgagg acgacgacaa cgtcgaagac
      421 atcgatctcg acgatctgtc ggcgatggaa gggccggacg cgtaacgcag tcggccgcgg
      481 ttcaccgcgc gatcggcggc cgcagcaata cggtcggcgg ttcgtgctcg ttctcgaggt
      541 cgaagcgcgc acatggctgg gtgccttcgg tggcgacgaa cagatacgag ggtaccggta
      601 gcagcttttg gagtgcgcgc tggacctcga tcgccgtcgg tcgctggccg cagtccttgg
      661 cgaccatgcg ctcgacgagc tcgacgagcc cgtagggcgc ctccggacag cgcggcgcga
      721 gtgctggcac gggacgattg agatgctgga ccaagatctc cacagagcta ctaccgacga
      781 acggcggtct gccggtcaac gcgtgatacg cgacgatacc gaggccgtag atgtccgagt
      841 aaccgtcgac cgtgccgccg cgcgcttgtt ccggagccat gtacgtcgga gtgccgatcg
      901 cttcgttgtg atgggtgtag cgcgcgccgg cgacatgatg ggcgatgccc cagtcgatca
      961 cgcgcacggg atacgtgcca tcggtgggtg tcagcaggat gttgtccggc ttgagatcac
     1021 ggtgcgtgac gccacgctcg tgcgccgcag cgagcacgcc ggcgacgttc gcgaccagct
     1081 cgatcaccgt gtcgtgcggc agcgtgcctt ccgcgatacg cagcgagagc gacgtgccct
     1141 ggatgagatc catcgcgatc cacggcctac catcgccgag cagaccgcat tcatagaact
     1201 gcgggatgcc gccgtgcgcg acgctcgcga gaatcgcggc ttcacgcatc atgcgatcga
     1261 tcgcgcgctc ttgccgcaac tgatcttcgc gcaagatctt gagcgcgacg acacgatcgt
     1321 cgatcacgtg gcgcgcacga aacacccagc ccatgccgcc ggtgccgagc aggcgctcga
     1381 tgcggtacgc ggaaaccagg tcgccggtcc ggagcgagcc ctcatgtcgt tcttcatgac
     1441 gctcctcgaa cgtgttcttt accgacgata cggccatgac gtcggttctc tgagcacgct
     1501 gcgtgccagc ccgctcgctg tgatcggtga aatgacaaaa ccgctccgca cagacactcc
     1561 tgacaggcac ttgcgctgag aatttgtcgg caagcttcct gcaaaacgca tcgggcatga
     1621 cctcagaccc cgtggacgag ctaggccccg acaaggtggt cggctcctac cgcatcctcc
     1681 gcgaaatcgg tcgcggcggt atgggcacgg tcttcgaggc gcgccacgtc gtgctgccca
     1741 gacgcgtggc tctcaaagta atgcacggcg agctgcgcaa gcatcccggc atggcgacgc
     1801 gcgtggtgca agaggcgtcg atcctcgagg aggttcgtca ccccggcgtc gtgcgcgtgt
     1861 tcgattgcaa catccttcct gatcatcggc cgtggatcgc gatggagctc gtcgaaggcg
     1921 agacgctcgc gaaccgcgtg ctgcacgagg gctgcctcgc gccgtacgaa gtcgcaacgc
     1981 tgctcgccga ggtcgccgat gtgctggctg ccgtgcacgc acgtggcgtg attcaccgcg
     2041 acctcaagcc cgacaacctg atcctcacgc cggcaaatcg tgacttcccg ctgcgcgtga
     2101 tcgattgggg cgtcgcacgg ctcggcccga tcgggaggct gactctcgac ggacttacgc
     2161 ctggaactcc gatctacatg tctcccgagc aggcgacggg acgaaacgtc gccgcaccgt
     2221 gtgacatcta cgcgctcggc gtgatcgcct atgaagcgct caccggtcat ccgccgttcg
     2281 acgggcgcac gctcgcggag gtcgtctcga tgcacctcac gagtgagccc gcgccgctcg
     2341 aagagcaatg ccgcgcgccc gccgagctgt gcgagctcgt gcatcgcatg ctcgacaagg
     2401 accagacgct gcgccccggc gcgatcgaag tccgtcaact cgcacgcacg ctcgcactcg
     2461 agctgtcgac cgcgtacgag agcatcgagg tcatcggggc agatgctccg cgtcttccca
     2521 aggccgccgt gcggcagcgc cggcacgtcg cactcgcgat cgaacggccg acagaagacg
     2581 cgcttatcgt cgacccagat gcactcgagt acggcgtcac cgagatggtg ccgacgattc
     2641 gtaaaccgcg ctggaccccc gaaatcccgg tcgcagcagc cgccgaagcg cgcgaggccg
     2701 cacgccctgc gatcacgccg aggggcatac gcgaccaggt cgccggcgaa atcgccgcca
     2761 cgaagcgtcg ctgacaatct cgtttgtagt catccgcgtt ggaccgagcg ggagcgcacg
     2821 cctgggcgca tgcgcacgtc ggattgcgga ccgcgcgcgc cgtgattgac acgtcgcgac
     2881 cacgacgcac ggcgatttcc gatcccccac cacgcacatc gcgacgctgc gcgcgtcgac
     2941 gcggggcacg cgtgttgctc ccctgcaaac cgattcacca ctaaccggag acccaccatg
     3001 cacaagaacg gatcgtcatc cttcgactcg cgagtcgact cgctgaagga aagcatgcgc
     3061 aacctcgtcg acgctggcgg tgagcgcgtc ggccatatca aggatcgcgc aatcggcgcc
     3121 aaggactcgg tcgtcgagag cggccaggcg gccctgtcgc gcatcagcgc gctcgtcaag
     3181 gagcatccgt tcgccgcact tggcatcgcg ttcggcgtcg gctacgtcgc ggttcgcctg
     3241 ctcaggaagt gaaggagaga acgatggaga cctacgagag ccaataccag cgcgagcaag
     3301 acatctacac cgagggcagg gccgctggca tcaagcgcaa gctcggcgac ttcggccgcc
     3361 gcatcaagcg catcgatgtg cgcacacgga tcacggacca tccattcgcg gcagtcggca
     3421 tcgcaatggg tatcggcgcg atcgtcgggc tcgcgagacc gatgccgaag cgtggtccga
     3481 tcgcgggtgc gatgatggcg atcgcgagca cgatcggatt ccgcatgctg cgcgaggccg
     3541 ccttgatcca gctcggccac tacgcgaagg gcttcctgaa caaggacgag cccggcatgc
     3601 agcagggcgt tccgcagggc tactagaaat gactgccggc cgcgatgccg atgctcgtac
     3661 cgacgagcgg catcgatagt gccggcatgt cgcgcggctg cgcggcgagc gagtaccagt
     3721 cgacgctttc gtcgacgaac acgctcgccg tgatcagcgg ccccttcttc gcgaagaaca
     3781 gagggaggaa cagaaagccg agcgcgcgaa cgcggccggt gagctggacg ccggtcgcga
     3841 cgccggcgcc gaagccggaa tagccatcga gcgcgccctc tgctctcgcg aagcgtggac
     3901 cgccgcgcac gtacatgacg aggtggtcgc ggatcgttgc gtgatagcga gcatcgaggc
     3961 cgtagctctc gaggtcggag tcacccatcg gtggatcgcc accgatgaac ccgagatagg
     4021 cgttgtcgcg ctgcactgcg atgtttcccg agatgagcgg cccgacggtg agcgcgccaa
     4081 atcgccaacc gagcgcgatc cgcacctgca acgacttgcc gagcacgtcg gcaccctcac
     4141 cgcgcgcgct cgagatgccg tacgactgcg aataatagaa gccgtcggcg tgagccgtgc
     4201 tggcagcgag cacgagcgcg agcgcagaga gccgcatacg caaagctcca gcaagtgctg
     4261 cgccagtagc gtgcgcgcac gtcagctagc tcacatgcat aacgctgcga agcggagccc
     4321 catggggaca gcaaaaaact gcaaccacca ggtgctcggt acccactgta gttggaccag
     4381 ggaggaccgc tcagatgact acccggctca cctacttcgt tctaccgcta ctcgttgcgt
     4441 gctcggacag taactcgtcg agtgatgtac tcggcggctc gctcacggtg accggcaccg
     4501 ttcatgactt cgaaagtgga gcgcaggtga tgggcgcggc gagcgtttcg accaccgcgc
     4561 tctcgccgac gccgaaggtc acggtcgaag gtgcagcatt cacgatcgat ggcgtgccgg
     4621 agaactcggc attccaggtg ctcgcgagtg tgccgacgag ccatcgcccg acgtacagcc
     4681 aggtcatcga agtcgtctcg gatgatgtcg atggcgtctc cgttaagacg gtgagcgaac
     4741 agttcctcgg tacgctcgcg accggctttg gcgtcacgcc atcggctgcg aagggcgtgc
     4801 tgctattgca cctggtcgat ggcgctggcg cgggcaaagc gggagtcgcc gcggcgaacc
     4861 tcgtgctcgc gaacgcggcg ggcgcgacgg gaccgaagtt tctcgacgcg aacatggcgc
     4921 cggcgccggc tgcgacggca agcacgtcgt cgggttgggc cgtgttcttc gaagtgcctc
     4981 cgggtacgat cgcgctcggc caggctgcga cggcaacggt gacgctcgag atggcgagct
     5041 cgccggttgg cagcggcgcg gtgacgatcg ccgacgtcaa ggtcaccgac ggtgcgccgc
     5101 ccgcgacgcc gacgaacgtt tcgttctcgc agcaagtgtt tccgatcttc acgaaccgcg
     5161 gctgcgtcgc gtgtcacacc ggtagcggtc caggcaagga cctcggcggc ctcatgctcg
     5221 atggcggctc gaacctcgcg tacaaggagc tgaccgtcga gaacccgctg cgcgcgaata
     5281 ccgcgacgcc ggaaaaaagc ctcatgctga cgatgccgtc ggccgaaagt ccgccggatg
     5341 gccatccgaa cgtgacgttc actggacctt cggaccctga ttacatcaag atcctggtgt
     5401 ggattcgcga gggagcgaag tccaactagg aggagccatg gttcgcttgt ttggggtcgc
     5461 gctggtcgcg atgactctgt cgtttgcgtg tagcgataat gcggatgatc tcggtgtcgg
     5521 cgcgcagtgc accggcaacg atcagtgcga cgccgacacg catcaggtct gcctgcagtt
     5581 caaaggcggc tactgcggcc tcatgggctg cgtacacgat acggattgtc cgcccgattc
     5641 cgcatgcatc atgcacacgg acggcatcaa ctattgcttc cggacctgcg tcgacaagcc
     5701 ggactgcaac gcgaaccgcg atgtcgacaa cgaggcgaac tgctcatcga gtgtgacgtt
     5761 cgtggatggc acgatgggcc gtaaggcctg cgttccgccg tcctagccag agtctgccgc
     5821 cggatcgacg tgccggccgg cacagacgcg gatatcttcg cgtcgtgcgt ctgttctgcg
     5881 cgctcggcct cgcgatcttg atcgcaagac cagcgcacgc ggacgacccg caggccatgc
     5941 ccgagcgggt tgtcgtcaaa cacgtcagcg cgaaacgacg tgctgctgcg gtcgcgctcg
     6001 cgatcgtgcc cggcatcgtt gtccatggca tcggcgcgtg gacggtgggc gagaaacgcg
     6061 ccgcgaagaa gctgttcgtc ggcgaggctg tcgggctcgg tgtggccgga ctatcgggac
     6121 tgctcgtcgg tggctccggt ggcaatccgt acaccgtgcc ggcggttccg atcgtggtcg
     6181 cgggcagcgg cttgatcctg cagtcgtgga tcgaagacat cttcatcgcc gccggcggcg
     6241 agaactatcg cggacaaccg cgcgcagtcg cgccgtggtc ggtcgagctc ggcacgtcgt
     6301 ggctgcacga cgcgtatcgc gagcgtgcgc tgctacgcgg cggtggccac atcgagttgt
     6361 tcggccgcgt cgtcgtgggt gcggaggcgc tcgtcgacgc gggtggcgac gcgaagatcg
     6421 gcgaggcaga cgcgcgcgtg cgtatcctcg gcgctccggc aacgggcgcg ttgatcgaag
     6481 acggcagccg gttgaccgcg cgcgttggat cgcgctggca cagagacgac gatgatcgcg
     6541 tgacgcaatg gacccaggag atcgggatcg acgggcggct cgatctcgat cgcatcgatc
     6601 gcgcgtttcg ccgcacgttc atcgaagcag gcgcgggcct cggaatcgtt cacgtgagct
     6661 acggcgacgt cgccaaggag tggtcgagcg agctgatcgc gcacttcgcg tggggcgcgt
     6721 acctgtgcac gcgcggtgaa gcgacggtgt tctacgagca cagccgcgat ggtctcgtgg
     6781 gtggcattcc ggcgtggcgc gcgtcgggct tcgtcggctc ggtcggcgcg accgtcgacg
     6841 tgcgcgtaca cggaccgtgg gctgtgcgcg gcgagctcga catcggcaac gcatatctga
     6901 cgacgctcgc cgtcgcgtat cgaggaggtc cgcaatgaag cacctggagc tcgcgctcgg
     6961 cgttgtcttg ctcgcggcct gcacgcgtcc cgcagaggag cgcgccaccc aagagctacc
     7021 ggtcggcagt gccgcgatca ccgatgcgtc ggtcgaagtt ggcggcggtc tcgccgcggt
     7081 gcgcgacctc acggatcatc ggctcgagct ctgggcgcag tcgcccgtgc tcgcgatcaa
     7141 tctcgtcgtc ggcgacaccg ccggtggcga ctggacgatc atcgtgcgca acacgctggt
     7201 cgatgcggtg ctcgacgacg gcggcacgat ctacgcgcgc gatcctgatc agcatccgac
     7261 ggtcggcatc ttccacgttc cgctcgcacc cggtagtcac gtcctgcacg tcgcaccacc
     7321 cgatgcggat acgatcgagc cgttccgcgt cgcggcgatg gccgacatcc agaccgcgct
     7381 ccccgaggtc gacgacatct tcgagatgat ctcggcggtg ccgaacgcgc gcttcgtgat
     7441 cgcgatgggc gacatcaccg aacgctcgca gaccgacgag tacgacctgt tcgagaagca
     7501 gctcgtcacg ttgacgattc cgttttacac gacgctcggc aaccacgagc tgtgggcgga
     7561 tccgtcgcgc tttttcgatc gcttcggccg cgcgagcttc cagttcgatt tcaaaggcgc
     7621 gtcgttcacg ttcgcggact ccggcgacgc cgggctcgat ccgctcgtcg aggattgggt
     7681 cgtcgattgg atcgcggccg cgaagaaccg cacgagcatc ttcctcacgc acattccgcc
     7741 gatcgatccg ctcggcgtgc gctacggcgg attccgcagc gcggaggatg gtcgccggct
     7801 gctcacgcgg ctcgccgaag cgaacgtcga cctcacgctg tacggccaca tccacacgta
     7861 cgtgaagttc gagaacgccg gcatcccagc gttcatctcg ggcggcggtg gcgccgaccc
     7921 gatgaagtgg gatgggatca atcgccactt tctcgtcgtg gacatcgatc ctgccgccgg
     7981 cgcgattgcc aacgtcgacg tcgtgcgcgt cgactgatca cttcttcgca ggcgcagcag
     8041 ccgaaccagc cgccggcgcc ttcgccggag cagccgagcc cgccgcggga gccttcgcag
     8101 ctgccggcgt cgcggccgct gccgagcccg ccgccttgtc ttccttcgcg gcgacgaagt
     8161 cgaacttcga cttctgccag acgtagaaga tgcaatcggc gcccggcgcg cacgccgaga
     8221 cgtgatcgac cttgcccggc atcatccagt atgagcccgg cgtcatcttc tttgccttcg
     8281 tgccgtcctc gcctttgagc cagtggctgc tcgtgccctg gacctcgatc gcctcgtacg
     8341 tgccggtgtg cgagtgcagc ggcgacgtga aacccgccgg gatcttgagc agcccgccat
     8401 acggcccctt cttgtagtca cccgtcagcg tgccgagctt cggaccaccg acgtcgtccc
     8461 acttcacttc ttcggccgcc gtgaacttcg gcgccggcgc agcgcccttg ccgactgcaa
     8521 cgcccgcgac gaacgcggcg ccgagcatcg aacccatgac cgtcaaaatt gcgagcttct
     8581 tcatgtcagc atcgaatcgc gtccgacgga tcgccgcaag agtgcatctc gcgaaccggc
     8641 cagggcattt cgccccacgc ggcattgtgc cccgcgcggc caaaatggca gcagcagcga
     8701 cggcgctccg atatgctgcg gacgtgctga cgagagagag ctgttacgtc gacgacgaca
     8761 tccgcagcgc cgaaggactg cccgcagccg cgttctccga tcgcgagttt ctcgcgcgcg
     8821 agctcgagac cgtcttcaag acgtcgtggc tcgtcgtgcc cgaacgcggc gatgatccac
     8881 gtccgctcga cgaacaagtc gccgcacgcg ggtcgcgcgt tccgatcacg gtcctcgatc
     8941 gcccgctgtt cgtgcagcgc ggttgggacg acgacacgct gcgcgcgttt ccgaacacgt
     9001 gcacgcacgc gtggtatccg ctcgtgctcg ggccgacgcg cggaccgaca ctcgtgtgcg
     9061 ggcaacatgg ccgccgcttc gactgcacgg gccgcttcgt gtcgcaacaa ggtttcaccg
     9121 gcgacatccc gaactttccg cgcgcgtgcg atcacctgca gcagcttgcg atcgcagcgt
     9181 ggcgccgcct gacgttcgtc aacatgggaa ccccggcggt gccgttcgac gagctattcc
     9241 gcgaagtcga cgcgtcgctc gccaacatgc ccccgatgcc cgagcgcatg tcggccgaga
     9301 tccgtgacgt cgcaggcaac tggaagcagc acgcctggaa ctttctcgat cagttccaca
     9361 tcggcttcgt gcatcgcgcg ccgggtgggc tcgcagacgc gatcgatctc ggctcgtaca
     9421 agaccgagct ctacgaccgc tcggtgctgc agtgggtcta cgcgcgcgaa gcaaacgacg
     9481 gtttcgatcc agcgtggctg cccgcgcgtt tccacgacga caagaagggc cgccgcgtgt
     9541 tcgcgctgtg gtggttcatc tatccaaacc tcgcgctcaa cttctatcca tggggcctgt
     9601 cggtgaacgt ctaccagccg gtacccgagc gtcccgagat gacgcgcttc gtctggtatc
     9661 agttcgcgct cgatcgccag aagcacgccg agcgcgatcg ccgctggctg tcatcgcagg
     9721 tcgacgccga agacgtcgac gcgctcgcac aagtcagccg cggtctgcgc agcgggtttg
     9781 cgccgcgcgg ccgcttcgcc cccgaccacg agcaagcagg acactggttc caccggctcg
     9841 tttcgcgcga cgtgctctcg acctgatcac atctcgtctt cgtcgggctc gatgtcgctg
     9901 tcgagcaccg cgagctcttg caacagcgcg cgaatctctg cgacgcgcgt ggacgacggt
     9961 ggccgcggct cttcaccgaa gccgcgttcg tcttcgagga caagcagcgc ctcgccgatc
    10021 tcgtcgggcg tagcaccggt ctcgaggatg cgttcgagga cgagcgggtc gacgtcaccg
    10081 agcagccggt tcacgtcgtt ggcagtggcg gaattcatcg gcatgcgcca agccactgca
    10141 tgcagcgcgc cactcagaac actgtggcag caagcaccat cagagattcc gagcgggctt
    10201 tcgcgaaaat cgtgcgcagc cgctcatcgg agatgctact tcgacgcagt gaacgtgtag
    10261 ctcacggtcc acggcaccgc ggattgtgga ttcgggtacc agctgcccgt gccggtcagg
    10321 ctcaatccgg tcgcggtctg cgtacccgtg acgttgtacg tgcgcttcaa gttgtcgagc
    10381 gtgttgtacg tgtgctcgac ggtgccggtc accgtgccgt cgagtctcac gttcgcgctg
    10441 atcgtcacgc gctgttgctg actgaacagc gtcatgccgc cgctgtacac gcacgtgaac
    10501 gtgccctgga tccccggttt gcgattgagc gccagcgccg cactgcccgt gccggtacaa
    10561 ctcatctcgt tgatcaccgt gctgccgagc ttctcctgcg tgagatggct gtacgagccg
    10621 gcgtacgaac cgacaagcgc tgtgcagcga ttcggctgcg cgactgtgcc gtccacaccg
    10681 ccatcgcaat cgttgtcggc gccatcgcag atctcggtcg cgcccggcca tgacgcgttg
    10741 tcgctatcgt tgcagtcgcc gccggcctcg acgtaaccgc tcggcgcgtt gcacgcgacc
    10801 atcgcagcac ccgcgccgaa gttgtcgtga tcttgatcgc gatagaacgt cttcgtcgac
    10861 gtcggatcga tgttcggatc cgcgatgtcg atcagattgt cgcagtcgtt gtcgatgtag
    10921 tcgcagatct ctttggcgcc cggattgatg gccgccatgt tgtcgttgca gtcgttcgag
    10981 ctcgcggcgt agccgaccgg cttcgcgcac gccttcttcg tcatcgcagc gtcgccataa
    11041 ccatcgttgt cggcatcgcg atagaacgta cccgtcgtcg tcatgtcgac ggagctgtcg
    11101 gcgtcatcga tcatgccgtc gcagtcgttg tcgatcgcgt cgcagatctc gcggccatcg
    11161 ggatgaatcg ccgcgttcgt gtcgttgcag tcgccaccca tcgcgatgaa gccggcaggc
    11221 gcttcgcaat gctcttcgcc cttcgccatg tcgccatagc cgtcgccgtc ggcgtccgga
    11281 taaaacgtct gcggcgtatc gcagctaccg acgtcgtcat cgccgccatc ttcagcggcg
    11341 cacgcggcta tgaatgtggc aaggaaagcg agcacgagga cggacgcgtt cttcatgccc
    11401 cgtaaacgca ggcgtgggcc cgcgtgggta ctttttctac aagcccgcgg ccttcttgat
    11461 ctcggcgatg ttgatgcgtt tcatcttcat cattgcttcc acggccttcg tcgccttgac
    11521 cttgtcgggg ccgctgatga ggtcgatgag gatcttgggc tggatctgcc agcgtagccc
    11581 ccaccggtcg gtaagccagc cgcagggtcc ttcctcaccg ccgccttcga tcagcttgtc
    11641 ggtcaggttg tcgacctctt gctgggtctc gaccgacacc gcgagcgaaa tcgcgtcggt
    11701 gaacttgaag tgttcgccac cgttgagcgc gacgtacgga atcccttcga gctcgaactc
    11761 gatggtcatc acggtgccct tcggcaacgg catgccctcg ccgtaaaagg tcttcttgcc
    11821 caccttcgag ctcttgaaga tcgaggcgta gtgctcgacg gcgccttcgg cgtctttttc
    11881 gtaggtcaga aacgtggtga ttttcggcat tgtcattctc cttgagcgaa ggacgaacct
    11941 gcagacctcg cgccgacact agaatcacga aaagtgaccg agccagctca tccccgcgaa
    12001 atcgcgatcg cccggtcggg agtgctgctc gcgttgctcg tgttcgcggc cccggcatcg
    12061 gccgacgagc taccagcgat tcggatcgag gcgaaggacc cagcggcggc caagcgcgcg
    12121 gtcgagctgt cgaaggcgct gcgcgcagtc gcggcgaaga aaacgaaaaa gtcgaagccg
    12181 accgcgaagg acgtcgacag cgcggtcgtc aacgccgagt gctcgatcgt gcagcccgca
    12241 tgcgccgccg aaatcggcga gaagctcgcg gtcacgcacg tgatggtcgg ccagctcgac
    12301 aagcgcggcg cgcgctacgc gctgacgctc tcgctcatca acgtgcgcac gaagcaacgc
    12361 gtgcgttcgc tgcgcgaggt gtcgacgacg aatgacatga agcgctgggc gaccacgctc
    12421 tacgcgcgca tgctcgacga gggcaccggc gaggtcgtga tcgtcgcgaa cgcgcgccgc
    12481 ggccaggtcc tcgtcgatgg cctcgccgtc accgagctct acgaaggtcg ggccacgctg
    12541 tcgggcgtcg cgctcggcac ccacgaggtc gagatccgcg cgagtggatt caagacgttc
    12601 gtgaccgacg tcgaggtcga tggccgctcg gagcagagct ttctgctcga tccggcgcaa
    12661 tagggaatgc acctcggcaa agtccggtta cccgtgcgaa ggtcacctcg tgcgtagcac
    12721 cgtccatacc gtcctcgtcc tcctcctcgc cgcgtgcggt cacgtcgaca cggcggcgcc
    12781 atctccgccg gcgactcttg catcggcgcc cacatcctcc gcaaccaagc cgacgtccga
    12841 agacgaacaa gggcgggccg ccgccgccac gctcgtcggc acgacgccgc cggagtggaa
    12901 ggccgagcgc tggatgaatt cgccgccgct cacgctcgcg agcctgcgcg gctcggtcgt
    12961 gctcgtacgg tggtggaccg cgggctgtcc gttctgcgcg gccaccgcgc cgtcgctgcg
    13021 cacgttcaat cgcagctacg ccgacaaagg tctcaaggtg atcggcctct atcaccacaa
    13081 ggacgacggc ccgttcgatc caaaggtcta cgaagacacg gccaagaaat acgagttcac
    13141 gttccccgtc gcggtcgatc ccgagtggca gacgctgcaa gcctggatga aggacaaggg
    13201 cggcaacacg gtcgagaccg gctttacaag cgtgacgttc gtgctcgaca agcacggcgt
    13261 cgttcgccac gtgcatccgg gtggccagta cgtcgccggc gaccccgcct acgccgagct
    13321 tcaagcggtg atcgagcgcc tgctcgccga gtcgtagcga tctaggatga cggcatgccg
    13381 aagccgccac ccacgcccgc tggcaagttg ctcgagctcg cagtcacgcc gtcgtatagc
    13441 cctcgcgtcg atggccgaac actgtgcatc cccgacggca actcgcagaa ccgtcgcttc
    13501 gacatcgaca ccggcaaagc gctcgccccg ctgccgttcg accgtacgtt gtggctgggc
    13561 acgggccgtg atcgcagcgt ccgcaagatc acggatgtcc tcgatgtcgt cgactccaag
    13621 ggcaacgtca tttgctcgta caaaggcatc ggttacgccc acgtcacgcc gtcgccggac
    13681 ggctcgatga tcgccatcca tcgcgtcaaa gagccacttc ggatcgcgag cgcgacggac
    13741 aaccagctcg tcgagattcc cgacggcaag atcaagcaga gctgcgaggt cgcgtggtcg
    13801 ccggacggat catatgtcgt cgtctactcg cacaccgagg cgatcgtgat cgacatcaag
    13861 gcgcgcaaag ccacgacgcg gctcaagcta cgtcatgtaa ctcgatgcct gttcgtgccg
    13921 aaccacaacg tcttcctcgc gggagattac aagcgcatca cgatctggga cccg
//
LOCUS       DASUFX010000055         6488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1814954, whole genome shotgun sequence.
ACCESSION   DASUFX010000055 DASUFX010000000
VERSION     DASUFX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814954"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFV99_02075"
     CDS             <1..1032
                     /locus_tag="VFV99_02075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058112.1"
                     /translation="AIKLIRPEVMHDDSGIDEVRERFRREAQTLASMKSRHTIELFDY
                     GVTAEGIFYYVMELLDGLDLESLVVRYGAQPSARVIDILAQACQSLAEAHDAGLFHRD
                     IKPPNLFLSRAADEVDIVKLLDFGIVQTVNETPSKPVQAAVAPAVDDTSKLTQLGAML
                     GTPGFMAPEQILGMQLDARADLYSLGCCAWWLLTGGEVFNREGGEAKVLHRHINDEVP
                     SLQDKVRGWLPDELEHIVRSLLAKEPEQRPNNARALAGQLRAIKIPDEYVWTQARAGA
                     WWRTYTPPAPVQPVPSAEVQVIMPGRTKEQRPLAATDERAIAATMAGPSVNTQLDRES
                     YPRIGGKDD"
     gene            complement(1165..3570)
                     /locus_tag="VFV99_02080"
     CDS             complement(1165..3570)
                     /locus_tag="VFV99_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5058113.1"
                     /translation="MPIRLALGSVVVFVACGNSSVEPMYDAPDGILDSPLSQPPDGFP
                     ADAEPDAWEPDTMPPFLASITPVTGTAVWLHAPIRLTFDEPIEATSIAMTVTATLAGS
                     AVPAQLAFEAPSTLIVTLAANVRGVGALDVGITGTLTDLAGNVYSSPIALSFVAPAWS
                     SIAIDRGYAASAPELAVAANGTLYAAWLVGNAGARRVVVSALVANTWESLGGALGASD
                     AGSVAIAIDGNGEPLVAWSEAAQAHVARWTGAWTELTSPGSANTVALVVPPNGEPLVG
                     LFGATAGVRELAGTAWQALGMDITLPSSIANTPCLAAGAARKPVIGWVDAQSQLRVYR
                     YDTSWTAITPIAVSAGSRMSLAARGTALAIAWDQYAGSYGVLAAQLSGVATTWTRLGR
                     ALDVDISGNAVAPAVAFDASGAPVVTWTELVETNQRGALARWTGSAWSVVGGVSWLDN
                     AVSSPMRSLIALHANEAAVVATSAAGTVRIARFNGPRIAAAGMTARASIAGCSFAAAN
                     PPALLSQTGCFNLATPNRPVPHAGLVPYDVVSELWSDGAKKRRYIGLPDNTGMTVSSN
                     GSWSAPVGTIMIKQFDLETTPGNPATRRPIETRFFVNDATLGWSGFSYKWNVAGTDAQ
                     LLTDGVWTFDWQMDDGTQHEHQYPSRSQCRSCHHSSMGPLLGVRSEQLARWNDYNGVI
                     ADQLQTLNALGIAPVASAMPFVSAQQPGETWERRMRGYMAGNCAHCHNPSNLSVKDLR
                     YTTPLAQTNLCSSISPGNPSGSQVYQLVTSRPGMPCLGTLAVDPLASQTLAAWISGMT
                     SCP"
     gene            complement(3672..4967)
                     /locus_tag="VFV99_02085"
     CDS             complement(3672..4967)
                     /locus_tag="VFV99_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058114.1"
                     /translation="MAAPKIHIVRTPDDTASELPTPATVERYQHGWRSGKPVEDPEKL
                     KRWGFIGAKPSEYLVCTRRGDVDRKRSGQGRRIFKWPWDSVSIVPTTLQRIEFTADQV
                     TKERVGVQVTGIAVFRIAEPLLAYRVLNFTYSEAASEKLAATLREMFVGSARRLIANL
                     SLEDCLTRRKEAIAGFLMDEIAPVVGGEGSPHDTTSKGWGVVIDTIEIQSVTIQSQAV
                     FAHLQAPFRAEIAARAELAELDRKRQVDAIEAERGLRQIQIAEQQRRAKVAAEVAVLA
                     AERARIEATHAQAAVDIEHERQATIARQAIELDTARNQADLSLELRRREADVTLELRK
                     RDAETKDQENQLEFEHQRRMAEIEKMLAQGRVMHDLVTTGLPQIAEALSQTFGTVNYT
                     QIGEGNGGPLQAVPAALAQLLALARSFGLELPRSDTQDE"
     gene            5112..>6488
                     /locus_tag="VFV99_02090"
     CDS             5112..>6488
                     /locus_tag="VFV99_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5058115.1"
                     /translation="MSAPFGVEARCERVSVSSGKSTLWASLRVDPKGKGLENDRAPLA
                     VCLVLDVSGSMSGDPIAHVLRASEIVTELLDARDQLAIVTFSNHAGVRCGLTKCDDDG
                     RKLIVSALRTIGVEGGTNMHAGMEAGAALLLSAPAGLRRTMVVLSDGQPNVGISSATQ
                     LAAYTQTLRPIGVSTLGFGLHHDENVLGAIAIAGSGRYAYIPDPIMARVDLARAALAH
                     GGIVAEKMEMELRLADGVELLRVLPMTQLRHGGTGVKASIGDVFVDEFRLLAFELSID
                     LKPSAKGNLAEVIVEGHTPAGATHKETVKLVVDVHGGPHTIDRKAQHEILIVRADFAR
                     SEARAYADRGAAPAAVSLLRDMIKQIEAADGFVRNDGSELAEMREQLEDEAQNYDRKA
                     SQAEMAHMRKGAMQYTPTAVGTQGARPRKQLHGAPGCLVGLSHDAQNQRYPLYTDTVL
                     GRSLDNDIP"
BASE COUNT         1044 a         2203 c         2178 g         1063 t
ORIGIN      
        1 gcgatcaagt tgatccgccc cgaggtgatg cacgatgaca gcggcatcga cgaggtgcgc
       61 gagcggtttc gccgcgaggc gcagacgctg gcctcgatga agtcgcgaca cacgatcgag
      121 ctcttcgatt acggcgtcac cgccgagggc atcttctatt acgtgatgga gctgctcgac
      181 ggtctcgatc tcgaatcgct cgtcgtgcgc tatggcgcgc aaccgtccgc gcgcgtgatc
      241 gacatcctcg cgcaagcgtg tcagtcgctc gccgaggcgc acgacgccgg cctgttccat
      301 cgcgatatca agccaccaaa cctgttcctg tcgcgtgccg ccgacgaggt cgacatcgtc
      361 aagctgctcg acttcggcat cgtgcagacc gtgaacgaga cgccgagcaa gccggtgcag
      421 gccgcggtcg cacccgcggt cgacgacacg agcaagctca cgcagctcgg cgcgatgctc
      481 ggcacccccg gcttcatggc accggagcag atcctcggca tgcaactcga cgcgcgcgcc
      541 gacctctact cgctcggttg ttgcgcgtgg tggctgctca ccggcggcga ggtgttcaac
      601 cgcgagggcg gcgaggcgaa ggtgctgcat cgtcacatca acgacgaggt gccatcgctg
      661 caggacaagg tccgcggctg gctgcctgac gagctcgagc acatcgtgcg ctcgctactc
      721 gcgaaggagc ccgagcaacg gccgaacaac gcgcgtgcac tcgccggcca gctgcgcgcg
      781 atcaagattc ccgacgagta cgtgtggacg caggcgcgtg ccggcgcgtg gtggcgcacg
      841 tacacaccgc ccgcgccggt gcaaccggtg ccgtcggccg aggtgcaggt catcatgccc
      901 ggccgcacga aggagcagcg gccactcgcc gcgaccgacg agcgcgcgat cgcggcgacg
      961 atggcgggtc cgagcgtgaa cacgcagctc gaccgcgagt cgtacccgcg catcggcggc
     1021 aaagacgatt aggcaccact gcgacgtcgc agacgggctt gcgggtgtcc gggagtgtcc
     1081 aacgtaaccc cggtacgttt cgggcaagct accgtcattc ctcgtgtccg gctgtccgaa
     1141 ttcgtccggt tttgtccggc gcgatcaggg gcacgaggtc atgccgctga tccacgcggc
     1201 cagcgtctgg cttgcgagtg gatcgacggc gagcgtaccg aggcacggca tgccgggccg
     1261 gctcgtgacg agttggtaga cctgcgagcc gctcggattg ccaggcgaga tcgacgagca
     1321 caggttcgtc tgcgcgagcg gcgtcgtgta ccgcagatct ttgacggaca ggttgctcgg
     1381 gttgtggcag tgcgcgcaat tgcccgccat gtagccgcgc atgcggcgct cccacgtctc
     1441 gccgggttgc tgcgcagata caaacggcat cgccgatgcg accggcgcga taccgagcgc
     1501 gttgagtgtc tgtagttgat cggcgatcac gccgttgtag tcgttccacc gcgcgagctg
     1561 ctccgagcgc acgccgagca gcgggcccat cgacgagtga tggcacgacc ggcactgcga
     1621 gcggctcggg tactggtgct cgtgctgggt gccgtcatcc atttgccagt cgaacgtcca
     1681 cacaccgtcg gtgagcagct gcgcgtccgt gccggccacg ttccacttgt agctaaagcc
     1741 gctccagccg agcgtggcgt cgttgacgaa gaagcgcgtc tcgatcgggc ggcgcgttgc
     1801 cggattgcct ggcgtcgttt cgagatcgaa ctgcttgatc atgatcgtgc cgaccggcgc
     1861 gctccacgag ccgttgctgc tcaccgtcat gccggtgttg tcgggcaacc cgatgtagcg
     1921 gcgcttcttc gcgccgtcgg accatagctc gctgacgacg tcgtacggca cgagtccggc
     1981 gtgcggcacc ggtcggttcg gcgtcgcgag gttgaagcag ccagtctgcg acagcagcgc
     2041 cggcggattc gccgcggcga acgaacagcc ggcgatcgac gcgcgcgcgg tcatgccggc
     2101 agcggcgatg cgcggaccat tgaagcgcgc gatgcgcacg gtgccagctg cactcgtcgc
     2161 cacgacggcg gcctcgttcg cgtgcagtgc gatcagcgag cgcatcggcg acgacactgc
     2221 attgtcgagc cagctcacgc cgccgacgac cgaccacgcc gagccggtcc accgcgctag
     2281 cgcaccgcgc tggttcgtct cgacgagctc ggtccacgtg acgaccggtg caccactcgc
     2341 atcgaacgcg accgccggcg cgacggcgtt gccgctgatg tcgacgtcga gtgcgcggcc
     2401 gagtcgcgtc cacgtggtcg cgacgccgga gagctgcgcg gcgagcacgc catacgagcc
     2461 cgcatattga tcccacgcga tcgcgagcgc ggtgccgcgc gcggcgaggc tcatgcgcga
     2521 gcccgccgac acggcgattg gtgtgatcgc ggtccagctc gtgtcgtagc gatagacgcg
     2581 gagctgactc tgcgcgtcga cccagccgat caccggcttc cttgctgcac ctgctgcgag
     2641 gcacggcgta ttcgcgatcg agctcggcag cgtgatgtcc atgccgagcg cttgccacgc
     2701 cgtgcccgcg agctcgcgca cacccgcagt cgcaccgaac agcccgacga gcggttcacc
     2761 gttcggtggc acgacaagcg cgacggtgtt ggcgctgccc ggcgacgtca gctcggtcca
     2821 cgcgccggtc cagcgcgcga cgtgggcctg cgcggcctcg ctccacgcga cgagtggctc
     2881 gccgttgccg tcgatcgcga tcgcgaccga gccggcgtcg ctcgcgccga gcgcaccgcc
     2941 gagcgactcc cacgtgttcg cgacgagcgc gctgacgacg acgcggcgtg caccggcatt
     3001 gccgacgagc cacgcagcgt acagcgtgcc gttcgctgcg accgcgagct cgggcgccga
     3061 ggccgcgtag ccgcgatcga tcgcgatgct gctccacgcc ggcgcgacga atgacagcgc
     3121 gatcggcgac gaatacacat tgcctgcgag atcggtcagc gtgccggtga tgccgacatc
     3181 gagcgcgccg acgccgcgca catttgcggc gagcgtgacg atgagtgtgc tcggcgcttc
     3241 gaacgcgagc tgcgcgggca ctgcactgcc ggcgagtgtc gcggtgacgg tcatcgcgat
     3301 gctggtcgcc tcgatcggct cgtcgaacgt gagccgaatc ggcgcgtgca gccacaccgc
     3361 ggtcccggtc accggcgtga tgctcgcgag aaatggaggc atcgtgtccg gttcccacgc
     3421 atcaggctcc gcgtcggccg gaaatccgtc gggcggctgg ctcaacgggc tgtcgaggat
     3481 gccgtcggga gcgtcgtaca tcggttcgac cgacgagtta ccgcaggcaa cgaagacgac
     3541 aacgctgccg agggcgaggc gaatcggcat gtcgggtggc acatcgcaag ccacatgcct
     3601 gcggtggccc ggccgctcag cttggttgga gcaccggcgt gtggcagcgg cgctcgaccg
     3661 cgcccgccgg ctcactcatc ttgcgtgtcc gagcgtggca gctcgaggcc gaagctacgc
     3721 gcgagcgcga gcagttgcgc gagtgccgcg ggtacggctt gcaacggacc gccgttcccc
     3781 tcgccgatct gggtgtaatt cacggtgccg aacgtttgcg agagcgcctc ggcgatttgc
     3841 ggcaggccgg tcgtgacgag gtcatgcatc acgcgtccct gcgcgagcat cttttcgatc
     3901 tccgccatcc ggcgttgatg ttcgaactcg agctggttct cctgatcctt ggtttcggcg
     3961 tcgcgcttgc gcagctcgag cgtgacatcg gcttcgcgcc tgcgcagctc gagcgagagg
     4021 tctgcttggt tgcgcgcggt gtcgagctcg atcgcctgac gcgcgatcgt agcttgccgc
     4081 tcgtgctcga tatccacagc tgcttgcgcg tgcgtcgctt cgattcgcgc gcgctcagct
     4141 gcgagcaccg cgacctcggc ggctaccttc gcgcgccgct gctgttccgc aatctggatc
     4201 tgacgtagac cacgctcggc ttcgatcgca tcgacctggc gcttgcggtc gagctccgcg
     4261 agctcggcac gcgcggcgat ctccgcgcga aacggcgcct ggaggtgcgc gaacaccgcc
     4321 tggctctgga tcgtcaccga ctggatctcg atcgtatcga tcacgacgcc ccagcccttc
     4381 gaggtcgtgt catgcggcga gccctccccc ccgacgacag gtgcgatctc gtccatcagg
     4441 aagccggcga tcgcttcctt gcggcgcgtg agacagtcct cgagcgacag gttcgcgatc
     4501 aagcgccgcg cggagccgac aaacatctcg cgcagcgtcg ccgccagctt ctcactcgct
     4561 gcctcgctgt aggtgaagtt gagcacgcga tacgcgagca gtggctcggc gatccggaag
     4621 acggcgatgc ccgtgacctg tacgccgacg cgttccttcg tcacctggtc ggccgtgaac
     4681 tcgatgcgct ggagcgtggt cggcacgatc gacaccgagt cccacggcca tttgaagatc
     4741 cgccggccct gccccgaccg cttgcgatcg acgtcgccac gccgcgtgca gacgaggtac
     4801 tcgctcggct tcgcgccgat aaagccccac cgcttgagct tttcgggatc ctcgacgggc
     4861 ttgccgctgc gccacccatg ttggtagcgc tcgacggttg ccggcgtcgg cagttcggaa
     4921 gcggtgtcgt cgggtgtgcg aacgatgtgg atctttggag cagccattgc gctgcacgat
     4981 ttgcagcgcg cgtgccgcgg caactacctg cgcaccggtg gagcagttgc tccgacgtgg
     5041 agcagttgca ccgtgatgga gcaccgaaaa atcacctagc gcttttccag cgcgacctca
     5101 tatttcgacg catgagcgca ccgtttgggg tggaagcgcg ctgcgagcgc gtcagtgttt
     5161 cgtccggcaa gtcgacgttg tgggcatcgc tgcgagtcga tcccaaaggc aaaggtctcg
     5221 agaacgaccg cgcgccactg gcggtctgcc tcgtgctcga tgtctcgggc tcgatgagcg
     5281 gcgatccgat cgcgcacgtg ctgcgcgcga gcgagatcgt caccgagcta ctcgacgcgc
     5341 gcgaccagct cgcgatcgtc acgttctcca accacgccgg tgtccgctgc ggcctgacga
     5401 agtgcgatga cgacggccgc aagctgatcg tctccgcgct gcgcacgatc ggcgtcgaag
     5461 gcggcacgaa catgcacgcc ggtatggaag caggcgccgc gctattgctc agcgcacccg
     5521 caggtctgcg ccgcacgatg gtcgtgctca gcgacggtca gcccaacgtc ggcatctcga
     5581 gcgcgacgca gctcgccgcg tacacgcaga cgctgcggcc aatcggcgtg tcgacgctcg
     5641 gcttcggtct gcaccacgac gagaacgtcc tcggtgcaat cgcgatcgct ggttccggcc
     5701 gctacgcgta catcccggat ccgatcatgg cgcgcgtcga tctcgcgcgt gcggcgctcg
     5761 cgcacggcgg catcgtcgcc gaaaagatgg agatggaact acggctcgct gacggcgtcg
     5821 agctgttgcg tgtgctgccg atgacccagc tccgccacgg cggcaccggt gtgaaggcat
     5881 cgatcggcga cgtgttcgtc gacgagttcc gcttgctcgc gttcgagctg tcgatcgatc
     5941 tgaagccatc cgcgaagggc aacctcgccg aggtgatcgt cgaaggccac acgcccgcag
     6001 gcgccacgca caaggagacc gtgaagctcg tcgtcgacgt gcatggcggt ccgcacacga
     6061 tcgaccgcaa ggcgcagcac gagatcctga tcgtccgcgc cgacttcgcg cgcagcgaag
     6121 cacgtgccta cgccgatcgc ggtgctgcgc ccgccgctgt ctcgctcctc cgcgacatga
     6181 tcaaacagat cgaagccgcc gatggcttcg tgcgcaacga cggctccgag ctcgcagaga
     6241 tgcgcgaaca gctcgaggac gaagcgcaga actacgatcg caaggcgtca caagccgaga
     6301 tggcgcacat gcgcaaaggc gcgatgcagt acaccccgac tgcagtgggt acgcagggcg
     6361 cccgcccgcg caagcagctc cacggcgcgc ccggctgcct cgtcggcctc agccacgatg
     6421 cccagaacca gcgttacccg ctctacacgg acacggtgct cggtcgcagc ctcgacaacg
     6481 acatcccg
//
LOCUS       DASUFX010000056         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1537556, whole genome shotgun sequence.
ACCESSION   DASUFX010000056 DASUFX010000000
VERSION     DASUFX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537556"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFV99_02095"
     CDS             <1..550
                     /locus_tag="VFV99_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5058116.1"
                     /translation="PSQAQIDAADAHPRVEYRREPAEKSSLADASVDLAVAAQAAHWF
                     DWPRYVAEVERVAKPGALVGLVSYGILNVEGDAGREIAHYYRDVVGPYWPPGRQHVEN
                     GYRDLAWPWPSVDAPAIDMTAQWTRDELVGYVTTWSATVKLVETVGPAPFEAFRTRLA
                     AVWPEDERRTVTWPLAIRLARR"
     gene            603..1514
                     /locus_tag="VFV99_02100"
     CDS             603..1514
                     /locus_tag="VFV99_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5058117.1"
                     /translation="MLLRFAHGPETIKLGDLEVRRLGFGAMRLPGKEVWGEPKDPATA
                     HAVLRRAIELGINFIDTAWYYGPLVANRLIVEALHPYPSDLVIATKLGGKRLPDKRWV
                     GAMTPEELRKGVDTDLETLKLEQLPVVHLRWIGEGAPISFTEALDVLIEQQRIGKIRH
                     LALSNVTVEQLEQAVAKTPIVAVQNMYNLAGGSGPGHIAADPEAVLAACEQKGIGFLP
                     FFPLAIGALAGGDSTLQTVATRLGCTPAQLAIAWLLARSKVMLPIPGTSKIAHLEENA
                     GAVHVALDEQAIRELTPHSTQQAAAAH"
     gene            complement(1524..1793)
                     /locus_tag="VFV99_02105"
     CDS             complement(1524..1793)
                     /locus_tag="VFV99_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058118.1"
                     /translation="MRALQFKETDRKTSSSTTTRGRKIRCPKCAWQPGKHDRWSCLCG
                     HSWNTFDTRGVCPGCDAKWSETQCRRCHEWSPHEAWYADDDDTPS"
     gene            complement(1813..>2549)
                     /locus_tag="VFV99_02110"
     CDS             complement(1813..>2549)
                     /locus_tag="VFV99_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058119.1"
                     /translation="PRLAPSTTHEGVAVMKSVAFCLPLLVACYGAAPPKPARIPLPPV
                     VEGGEIAVHTESRTTIESVAKEASTCPEGHSPGDPLCTVTRYSVNEPVTRTHSSASYA
                     DEPISYAQFKVMTDPQYDAKLSRLEDLSHRCKRANVPRYVGLGLMLGSLIAAPIVGKA
                     TDSTAASQGTLWGMLGLGGASFALGYFSFGGRDCNEARGIYNDVNVAGYVDTHDLLGA
                     DTAAEMSELAARFNANQTRQSAMRMR"
BASE COUNT          416 a          843 c          877 g          413 t
ORIGIN      
        1 accgtcacag gcgcagatcg acgccgccga cgctcatccg cgcgtcgagt accgccgcga
       61 gcccgcggag aagagctcgc tcgccgatgc gagcgtcgat ctcgcagtcg ctgcgcaggc
      121 ggcacactgg ttcgactggc cgcgctacgt cgccgaggtc gagcgcgtcg cgaagcccgg
      181 tgcgctcgtc gggctcgtca gctacggcat cctgaacgtc gagggcgacg ccggccgcga
      241 gatcgcgcac tactaccgcg acgtcgtcgg gccctactgg ccgcccggtc gccagcacgt
      301 cgagaacggc tatcgcgatc tcgcatggcc gtggccgagc gtcgatgcgc ctgcgatcga
      361 catgacggcg cagtggacgc gcgacgagct cgttggctac gtcacgacgt ggtcggcgac
      421 tgtcaagctg gtcgaaacgg tcgggccggc gccgttcgaa gcgtttcgca cgcgcctcgc
      481 tgcggtgtgg cctgaggacg agcggcgcac tgtgacgtgg ccgctcgcta tacgactcgc
      541 gcgccggtaa tcacggttgg aaaccggcgc ggacgcacgc gcgtcgtggc aaggtcgtag
      601 tgatgctgct gcgctttgcc cacggcccgg aaaccatcaa gctcggcgat ctcgaagttc
      661 gtcgcctcgg cttcggcgcg atgcggctgc cgggcaaaga ggtgtggggc gagccgaaag
      721 atccggcaac cgcgcacgcg gtgctgcggc gggcgatcga gctcggcatc aacttcatcg
      781 acaccgcctg gtactacgga ccgctcgtcg cgaatcgctt gatcgtcgag gcgctgcacc
      841 cgtacccgag cgatctcgtg atcgcgacga agctcggcgg caagcgcctg cccgacaagc
      901 gctgggtcgg cgcgatgacg cccgaggagc tgcgcaaagg cgtcgatacc gacctcgaga
      961 cgctgaagct cgagcagctc cccgtcgtgc acctgcgctg gatcggcgag ggcgcaccga
     1021 tctcgtttac cgaggcgctc gacgtgctca tcgagcagca acgcatcggc aagatccgtc
     1081 acctcgcgct cagcaacgtc acggtcgagc agctcgagca ggcggtcgcg aagacgccga
     1141 tcgtcgcggt acagaacatg tacaacctcg ccggtggcag cggcccgggc cacatcgctg
     1201 cggatcccga ggcggtgctc gccgcgtgcg agcagaaggg catcgggttc ctgccgttct
     1261 tcccgctcgc gatcggcgcg ctcgccggtg gcgactcgac gctgcagacg gtcgcgaccc
     1321 gactcggctg cacgccagcg cagctcgcga tcgcgtggct gctcgcgcgc tcgaaggtga
     1381 tgttgccgat tccgggcacc agcaagatcg ctcacctcga ggaaaacgca ggcgctgttc
     1441 acgtcgcgct cgacgagcaa gcgatccgcg agctcacgcc gcattcgacg cagcaggctg
     1501 ccgcggcgca ctaggtacga gcactagctc ggagtgtcgt cgtcgtcggc gtaccacgcc
     1561 tcgtgcggcg accactcgtg gcagcggcga cactgggtct cgctccactt cgcatcgcag
     1621 ccgggacaca cgccgcgcgt gtcgaaggtg ttccagctgt gaccgcagag gcagctccag
     1681 cgatcgtgct tgcctggctg ccacgcgcat ttcgggcagc gaatcttgcg cccgcgcgtc
     1741 gtcgtcgatg acgacgtctt gcgatcggtc tctttgaact ggagcgcgcg cacggctcga
     1801 ttgtagcgaa cgtcagcgca tgcgcatcgc ggattgacgg gtctggttcg cgttgaaccg
     1861 cgccgcgagc tccgacatct cggcggcggt gtcggcaccg agcaggtcgt gcgtgtcgac
     1921 gtagccggcg acgttcacat cgttatagat cccacgcgct tcgttgcaat cacgaccgcc
     1981 gaacgagaag taaccgaggg caaacgacgc gccgccgagc ccgagcatgc cccacagcgt
     2041 tccttgcgaa gctgctgtgc tgtcggtggc tttgcccacg atgggagcgg cgatgaggga
     2101 gccaagcatg aggccgagcc cgacgtagcg gggcacgttt gcgcgcttgc agcgatgaga
     2161 aagatcctcc agtcgcgaaa gcttcgcgtc gtattgtgga tcggtcatga ccttgaactg
     2221 cgcgtacgag atcggttcgt ccgcatacga cgccgacgag tgcgtgcgcg tgaccggctc
     2281 gttcaccgag tagcgcgtga ccgtgcagag cgggtcgccg ggcgagtgcc cctcggggca
     2341 ggtgctcgct tcctttgcca cgctctcgat cgtggtgcgg gattcggtgt ggaccgcgat
     2401 ctcaccaccc tcgacgaccg gcggtagcgg gatgcgagcg ggcttcggcg gtgcagcgcc
     2461 gtagcaggcg acgagtagcg gcaagcagaa cgcgacggac ttcatgacgg cgaccccttc
     2521 gtgtgttgtc gaaggtgcga gccgcggcc
//
LOCUS       DASUFX010000057         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_564867,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000057 DASUFX010000000
VERSION     DASUFX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564867"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VFV99_02115"
     CDS             <1..1359
                     /locus_tag="VFV99_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5058120.1"
                     /translation="FPLAEYLPYAVTRTWHTQLGIFWIATAWLATGLFIAPAVSGYEP
                     KFQRLGVNTLFACLLVIVVGSMAGQWFAIQQRLGFVANFWFGHQGYEYVDLGRFWQLF
                     LFAGLFIWLGLMVRAMWPAIVRPSESRHLLALFLLSSLAIAMFYGAGLMWGRHTHLAV
                     AEYWRWWVVHLWVEGFFEVFATVIIAFLFARLGLVRHSIAAAATVLAATIFLAGGVIG
                     TLHHLYFTGTPTPALALGSVFSALEVVPLLFLGFDAWDNWKHTRTTPWMQSYRWPIYF
                     FIAVAFWNLVGAGLFGFMINPPIALYYMQGLNTTPVHGHTALFGVYGMLGMGLILFTV
                     KAIHPHLVWPERLMKLAFWAINIGLGAMVVISVLPVGLMQTWAAVDVGYWYARSPEFL
                     DNPVVAGFHWSRVFGDTIFAIGALSFVVAVAKLSIHYKRQEARDEARAGERLPTARTA
                     RK"
     gene            complement(1362..2033)
                     /locus_tag="VFV99_02120"
     CDS             complement(1362..2033)
                     /locus_tag="VFV99_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEX5058121.1"
                     /translation="MLDPTTSVSAIVLDHSETAAVFSRHKIDYCCRGERSLADVCTTA
                     GLDLQDVLGELETAIARRQPKTVDPRSLSTRDVIVKLIAPHHQFLHRNLPFVQSLATK
                     VARVHGDKEPSLRELSRLVDGLVSSLTVHLEEEEQILFPALLGMSDADPTTMLADMRN
                     EHLAVGAMLAQIRMTAVDFTPPSWACNSYRTLLKELAEIERDTLEHVHIENHVLAPRF
                     MAPAS"
     gene            complement(2037..2660)
                     /locus_tag="VFV99_02125"
     CDS             complement(2037..2660)
                     /locus_tag="VFV99_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058122.1"
                     /translation="MLAFAGWRAAPGARLAKRACAALSHRVENFSPHALRQASERWED
                     HLFLAREVWRKVALQSIHDARELACDCGNLSRIGDQSLADRNQRRHERLVGSVLAGHQ
                     HERRSGCLVIRETRRDLFVLVGDVHRQRFREVAPDLRRNVADLVGRNVGGSGQCPRHR
                     EEPADALVAFEQELDDIAGLGTLTDLYEHGLVLERCSDYAGTIRAPQ"
     gene            complement(2668..>3249)
                     /locus_tag="VFV99_02130"
     CDS             complement(2668..>3249)
                     /locus_tag="VFV99_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HEX5058123.1"
                     /translation="GKRLRDQQGREAARRLVLLASVAAAAGIAGLVLAPAVLTTAALV
                     AIPALLTIALAWRRLERTLVGEIGAAVALSGAAAPVLVASGVGQRAAVATWGAWAIGY
                     ACTVIAVHRVIARHRTAATWRDTLVIVVLLAVTIASAVLLAHGSLVGAATPLALAAMA
                     LVIHPPKATYLRTIGVALVVASVATGALAVAAL"
BASE COUNT          502 a         1088 c         1083 g          576 t
ORIGIN      
        1 ttcccgctgg cggagtacct gccttacgcc gtcacgcgca cctggcacac gcagctcggc
       61 atcttctgga tcgccacggc ctggctggcg acgggcctgt ttatcgcccc ggccgtttcg
      121 gggtacgagc cgaagttcca gcgcctcggc gtcaacacgc tcttcgcctg cctgctggtg
      181 atcgtcgtcg gctcgatggc cgggcagtgg ttcgccatcc agcagcgcct cggcttcgtc
      241 gccaatttct ggttcggcca ccagggctac gagtacgtcg acctcgggcg cttctggcaa
      301 ctgttcctgt tcgcggggct gttcatctgg ctggggctga tggtccgcgc gatgtggccc
      361 gccatcgtcc gccccagcga gagccgccac ttgctggccc tgttcctgct cagctccctg
      421 gccatcgcaa tgttttacgg cgccggcctg atgtggggcc ggcacaccca tctggccgtc
      481 gccgaatact ggcggtggtg ggtggtgcac ctgtgggtcg agggcttctt cgaagtgttc
      541 gcgacggtga tcatcgcgtt cttgttcgcg cggctcggtc tcgtgcgcca ctcgatcgcg
      601 gcggcggcga ccgtgctcgc ggcgacgatc tttctcgccg gcggtgtgat cggcacgctg
      661 caccatctct acttcacggg cacgccgaca cccgcgcttg cgctcggctc ggtgttctcg
      721 gcgctcgaag tcgtgccgct gctgttcctc ggcttcgacg cctgggataa ctggaagcac
      781 acgcggacga cgccgtggat gcagtcgtat cgctggccga tctacttctt catcgcggtc
      841 gcgttctgga accttgtcgg tgccggtctg ttcggcttca tgatcaaccc gccgatcgcg
      901 ctctattaca tgcagggcct caacaccacg ccggtgcacg ggcacaccgc gctgttcggc
      961 gtctacggca tgctcggcat gggcctcatc ctgttcaccg tcaaggcgat ccatccccac
     1021 ctcgtatggc ccgagcgcct gatgaagctc gcgttctggg cgatcaacat cggcctcggc
     1081 gcgatggtcg tgatcagcgt actgccggtc ggcctgatgc agacgtgggc tgcggtggac
     1141 gtcggctact ggtacgcgcg cagccccgag ttcctcgaca atcctgtcgt ggccggcttc
     1201 cactggtcgc gtgtgttcgg tgacacgatc tttgcgatcg gcgcgctctc gttcgtggtc
     1261 gcggtggcca agctgagcat ccactacaag cggcaagaag cgcgcgacga ggcgcgcgcc
     1321 ggcgagcgct taccgaccgc acgcacggcg cggaagtgat ctcatgacgc cggtgccatg
     1381 aatcgcggtg cgagcacgtg gttctcgata tggacgtgct cgagcgtgtc gcgctcgatc
     1441 tcggcaagct ccttgagcag cgtgcggtag ctgttgcacg cccacgacgg cggtgtgaag
     1501 tcgaccgccg tcatccggat ctgcgcgagc atcgcgccga ccgcgaggtg ctcgttgcgc
     1561 atgtcggcca gcatcgtcgt cgggtctgcg tcgctcatgc cgagcagcgc gggaaacagg
     1621 atctgctctt cctcctcgag gtgcacggtt agcgaggaga cgagcccatc gacgagacgc
     1681 gatagctcgc gcagcgatgg ctccttgtca ccgtgtacgc gtgcaacctt ggtcgccagc
     1741 gactgcacga acggcaggtt gcgatgcagg aactgatgat ggggcgcgat cagcttcacg
     1801 atcacgtcgc gcgtcgacag cgatcgcggg tcgacggtct tgggctgacg tcgcgcgatc
     1861 gcagtctcga gctcgccgag gacgtcctgc agatcgagac ccgcggtcgt acatacgtcg
     1921 gcgagcgacc gctctccgcg gcagcagtag tcgatcttat gacgcgagaa cacagcggcc
     1981 gtctccgagt ggtcgagaac gatcgccgaa acagaggtgg ttgggtccag catgcgtcat
     2041 tgcggagcac gtattgtgcc ggcatagtca ctgcagcgtt cgaggaccaa accatgctcg
     2101 tacagatcgg taagcgttcc gagacccgcg atgtcgtcga gctcctgctc gaatgccacg
     2161 agcgcatccg caggttcctc gcgatggcgc gggcattgac cgctgccgcc gacgttcctg
     2221 ccgaccaggt ccgcgacgtt gcgtcgcagg tcaggcgcta cttcgcggaa tcgttgccga
     2281 tgcacatcgc cgacgaggac gaagagatcg cgccgcgtct cgcgaatcac gaggcagccg
     2341 ctgcggcgct cgtgctgatg accagcgagc acgctaccca cgagccgttc gtggcggcgc
     2401 tgattgcggt ctgcgagcga ttgatcgccg atccgcgaca ggttgccgca gtcgcaggcg
     2461 agctcacggg catcgtggat cgactggagc gcgactttgc gccacacctc gcgcgcgagg
     2521 aacaagtgat cttcccagcg ctcgctcgcc tgccggagcg cgtgcgggga gaaattctcg
     2581 acgcgatgcg aaagcgccgc gcacgcgcgc ttggctaaac gcgcaccggg tgccgctcgc
     2641 cagccggcga acgcaagcac tgcgcgttta cagcgcggcg accgcgagcg cgccagtggc
     2701 caccgacgcg acgacgagcg cgacgccgat cgttcgtaag tacgtggcct ttggtggatg
     2761 gatgacgagg gccatcgcgg caagtgcgag tggtgtcgcc gcgccgacca gcgagccgtg
     2821 tgcgagcagc actgcgctgg cgatggtcac cgcgagcagc accacgatca ccagcgtgtc
     2881 acgccacgtc gccgcggtgc gatgccgcgc gatcacgcga tggacggcaa tcaccgtgca
     2941 cgcgtagcca atcgcccacg cgccccaggt cgcgaccgcg gcacgctgcc caacgccgga
     3001 cgcaacgagc accggcgcgg ccgcacccga cagcgcgacg gcagcgccga tctcaccgac
     3061 gagcgtgcgc tcgagccgcc gccacgcgag tgcgatcgtc aacagggcag ggatggcgac
     3121 gagcgcggcg gtcgtcagca cggccggcgc gagcacgagc ccggcaatgc ctgccgcagc
     3181 ggcgacgctc gcgagcagca cgagtcgccg tgcagcctcg cggccttgct ggtcgcgtaa
     3241 ccgcttgcc
//
LOCUS       DASUFX010000058         7334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1815776, whole genome shotgun sequence.
ACCESSION   DASUFX010000058 DASUFX010000000
VERSION     DASUFX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815776"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFV99_02135"
     CDS             complement(<1..661)
                     /locus_tag="VFV99_02135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058124.1"
                     /translation="MARCTTCHRRLRSGQPCPLHGGASAEVQHSAAAAFEWKEPVGAL
                     IGSGGFASVWALDGNRVIKIAHASHDLARARMAREAEALRAIGAPAVPHCHDSGVLAD
                     GRAWVVMDRVAGANLADLACDAPLRSAEVVALGLACLDALERVHAVQFVHRDIKPDNM
                     VRTKEGRVVILDLGLARKLPDDPDDPTRANVQVGSLEYIAPEQIADSANVDERSDIYA
                     LG"
     gene            703..1827
                     /locus_tag="VFV99_02140"
     CDS             703..1827
                     /locus_tag="VFV99_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058125.1"
                     /translation="MLEFVIEAMVDAVMVIDAEGRITLANSGATQLTNYSREELRGMS
                     VSTLLVDDSSGLRTVTRRRIEDGDVLRREESWLVTKESERIPVSITGSPVVNDQGTLQ
                     GIVLVARDVREMRGLLADKEAEISRRRKAEDELRAAKASIEDKLDQMRTQLLLAERRA
                     TLGTLAGGVGHELRNIAQIQIAALDELASTLRADDKLRTILAELERVGEHITAHGNRL
                     MQLARPGPQHVSPIDLNNVVRDVAAMLKLAGKLGRVELVLSLPAAPVIVTVNRTSIEQ
                     IFVNLIINAVDATSESGTITVEVRPTGDRVTCGVSDTGVGIAGDQFEKIFEPFYTTKG
                     DLGTGLGLPVVREIVTSYGGDIKIDSTVGAGTTFTFDLPV"
     gene            complement(2142..2756)
                     /locus_tag="VFV99_02145"
     CDS             complement(2142..2756)
                     /locus_tag="VFV99_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX5058126.1"
                     /translation="MVLTVLGGVAAVGIATNERRKALAGGGGGQKQLGAGGGDKLLER
                     TVRDLRVDDVLTIDGKDFICEGAILYDEDGHRWVAGRCVDGTDIKWVLVGIDRAGTSA
                     TRLLTQDESTPIAGYPPEAIVVGETRYALDKRGTATCTLKGDVGPLGVGTKDRPEGHT
                     ERCRWWLYSAPGEDTMLVEQWGSDYRVLRGKKVGDATIELMPGS"
     gene            2861..3973
                     /gene="pdhA"
                     /locus_tag="VFV99_02150"
     CDS             2861..3973
                     /gene="pdhA"
                     /locus_tag="VFV99_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5058127.1"
                     /translation="MVAVPMQDDLRRVLDDDGKPLPGAKVPVIPDATLQRIFDTMMLV
                     RIMDDRMMRLQRQGRLGFYMKSIGEEASHFAVAPLRDSDWIFPSYREQGAWFWRGYKV
                     QQFIDQMFGNVDDPIKGRQMPVHHSANWLNLVSISSPVGTQIPQAVGAAYAAKVSGKD
                     DVAMVYFGEGTSSTGEFHVGMNFAGVWKAPCVFICRNNGWAISVPREKQTAAKTFAAK
                     AVGYGMPGIRVDGNDLLAIWQVASEAIERARAGEGPTLIEALTYRVQGHSSSDDPSVY
                     RDPKEPEIWEKKDPINRLRAYMRHRGLWSESWEKELSEKYNQEITDALAAADHKGAPA
                     VETMFDDVYEEMPWNLREQREWLMHQARTKNPHHHG"
     gene            3989..4954
                     /locus_tag="VFV99_02155"
     CDS             3989..4954
                     /locus_tag="VFV99_02155"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5058128.1"
                     /translation="MNIIEAVRNALQLQMRQDQRVVVLGEDVGKFGGVFRATSGLYDE
                     FGADRVIDTPLAEAGIIGTAIGMALYGLRPVPEIQFGDFIFPAFDQIVNELAKFRYRS
                     GGQYPCPVVIRTPVGGGIRGGHYHSQSPESLFIHTPGLKVVAPSNPYDAKGLLLACMR
                     QNDPVLFMEPKRIYRASKGEVPESDYVIELGKAAVTREGNQITLIAWSGMVSIAEEAA
                     KKAAEAGMSVEVIDMRTLMPFDIDTILESVKKTGRCVIVHEAPRTCGFGAELIASIQE
                     RALDSLEAPIVRVTGFDTPFPYSLEHEYMPNADRVLGALRQTLEW"
     gene            4957..6297
                     /locus_tag="VFV99_02160"
     CDS             4957..6297
                     /locus_tag="VFV99_02160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5058129.1"
                     /translation="MAFDFYMPDIGEGVVEGEIVGWKVKEGDRVKLDQPIVEVMTDKA
                     TVELPSPRAGVIKKINYKDGEICPVGKVLVVIDEEGASAAATPAAGGNGKKQPAMIVE
                     EPPRKAPTTAQVMHSKVAQNVPLTSPTSSSASSSGAIQVVDATADRERVLATPATRRL
                     ARQLGVEIGRVPATGKHGRVTTDDVKRFKEAPSGAPAAQARTAYTPVSIAKGGDEERI
                     PLRGIRKKIAESMTRSVQTAAHFTYVEEVDMTELVAVRDRAKSRATERGVKLNYLPFI
                     VKAVVSGLKKWPMLNASLDESTQEIVRKKYYHIGIAAQGPQGLAVSVVRDADKRSIFD
                     LSKEIERLGEAVRTGTATREELTGSTFTISSLGKLGGVLATPIINFPEVAIIGVHKIE
                     ERPAVRNGQIVARHLMNLSISVDHRLADGWDGAMFLQDVKALLEDPTTMFMEMV"
     gene            6322..>7334
                     /locus_tag="VFV99_02165"
     CDS             6322..>7334
                     /locus_tag="VFV99_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058130.1"
                     /translation="MTKKGSGTRDSRSEPDSEGASENFRVDRVFAPTRPASQSRPPMS
                     RPVTKPPTMPPSPMGTTPPATIAAGDSETELALRRQLSRLQRQLADAQRELANKDEEV
                     AAAVEKRIEIQQAYDVILDEQRHTRTLVDEAIADRSRTTGVEQRLQEALAAADELRHQ
                     LERERTERATLAVQFDEANAAFEKSRTAWREESATIDEQHIAQIAQLEQQKRAAVDAA
                     EAAMKTSTERQFQAHEAELDALRASHQRELAALRGEIEPKVSEARNLSAEIERLSAQL
                     QAQAAEHQSLLAERIELQTWELQQQAEANAADLAALQRQHATEVARLNEEVAAANQAG
                     QLI"
BASE COUNT         1398 a         2428 c         2370 g         1138 t
ORIGIN      
        1 agccgagcgc gtagatgtcg ctgcgctcgt cgacattcgc cgagtccgcg atctgttcgg
       61 gcgcgatgta ctcgagcgag ccgacttgca cgttcgcgcg cgtcggatca tccggatcgt
      121 cgggcagctt gcgcgcgagc ccgaggtcga gaatcacgac acgcccttcc ttcgtacgca
      181 ccatgttgtc gggcttgatg tcgcgatgca cgaattgcac ggcatgcacg cgctcgagtg
      241 catcgagaca tgcgagcccg agtgccacca cttccgcaga tcgcagcggg gcatcacacg
      301 caagatctgc gaggttcgcg cccgccaccc gatccatcac gacccacgcg cgcccatctg
      361 cgagcacgcc actgtcatga cagtgtggaa cagcaggagc gcctatcgca cgcagcgctt
      421 ccgcttcgcg agccatgcgc gcacgagcca gatcgtggct cgcgtgcgcg atcttgatca
      481 cgcgattgcc atcaagtgcc cacaccgaag cgaagccgcc gctaccaata agagcaccaa
      541 ccggttcttt ccattcgaat gcagctgccg cggaatgttg cacctctgca ctcgcgccac
      601 cgtgaagtgg gcatggctgt ccggatcgca gacgtcgatg gcaggtggtg caacgcgcca
      661 ctgcctcgac tgtaacgctc gcgtgctagt gtggtcattg aagtgctcga attcgtcatc
      721 gaggcgatgg tcgacgccgt tatggtgatc gacgccgagg gacgcatcac gctggcgaat
      781 tccggcgcga cgcagctcac caactattcc cgcgaggagc tacgcggcat gtcggtctcc
      841 acgctactcg tcgacgacag ctcgggcctg cgcactgtca cccggcgtcg catcgaagac
      901 ggcgatgtac tccgccgcga ggaatcgtgg ctcgtcacga aggagagcga gcgcattccg
      961 gtttcgatca ccggctcgcc ggtcgtcaac gatcagggca cgctacaagg catcgtgctc
     1021 gtcgcgcgcg acgtgcgcga gatgcgtggc ctcctcgccg acaaggaagc cgaaatctcg
     1081 cgccgccgca aagccgaaga cgagctgcgc gccgccaaag catcgatcga ggacaagctc
     1141 gatcaaatgc gcacgcagct actgctcgcc gaacgccgcg cgacgctcgg cacgctcgcc
     1201 ggtggcgtcg gtcacgagct gcgaaacatc gcccagatcc agatcgcagc actcgacgag
     1261 ctcgcgagca cgctccgcgc tgacgacaag ctgcgcacga tcctcgccga gctcgagcgc
     1321 gtcggtgagc acatcaccgc gcacggcaat cggctgatgc agctcgcccg ccccggcccg
     1381 cagcacgtgt caccgatcga tctcaataat gtagtgcgcg acgtcgccgc gatgctcaag
     1441 ctcgccggca agctcggccg cgtcgagctc gtgctgtcgc tgcccgccgc gccggtgatc
     1501 gtcaccgtca atcgcacgag catcgagcag atcttcgtca acctgatcat caacgcggtc
     1561 gacgcgacga gcgaatccgg caccatcacg gtcgaggtgc ggccgaccgg tgaccgcgtc
     1621 acctgcggcg tctccgacac cggcgtcggc atcgccggcg accagttcga aaagatcttc
     1681 gagccgttct acacgaccaa gggcgacctc ggcactgggc tcggccttcc cgtcgtgcgc
     1741 gagatcgtca cgagctacgg gggcgacatc aaaatcgaca gcacggtcgg cgccgggacg
     1801 acgtttacgt tcgaccttcc ggtgtagctc accgccaaga cgcagcagag gccaagcacg
     1861 cagctcggct atgtgccgca gagatcaccg ccaagacgcc gcagacgcca agctcagaga
     1921 tcgagttcga tccgcgccgt gctcgcgcga gcggccgccc acagtctggt cggcaccgag
     1981 gtttgtgatc gagcacagcg cgcgtacgag cagagcgcgc accgagatca gtttctctgg
     2041 ccttggcgtc tgcggcgtct tggcggttat cgagtcttct ggctgctcga tcttacctcg
     2101 cacgtcagcc ctgacgtctg cggcgtcttg gcggttgggc gctagctgcc cggcatcagc
     2161 tcgatcgtcg cgtcgccgac cttcttgcca cgcagcacgc gatagtccga gccccactgc
     2221 tccacgagca tggtgtcctc gccgggcgcg ctgtacagcc accagcggca gcgctcggta
     2281 tgcccctccg ggcgatcctt ggtgccgact ccgagtggtc cgacatctcc tttgagcgtg
     2341 cacgtcgcgg tcccgcgctt gtcgagcgcg tagcgcgtct cacccaccac gatggcttcg
     2401 ggcgggtagc cggcgatcgg ggtcgattcg tcctgcgtga gcagccgcgt cgcgctcgtt
     2461 ccggcgcggt cgataccgac cagtacccat tttatgtccg taccgtcgac gcaccggccc
     2521 gcgacccagc ggtggccatc ctcgtcgtac aggatggcgc cctcacaaat gaagtccttg
     2581 ccgtcgatcg tgaggacgtc atccacccgc agatctcgca cagtccgttc caggagcttg
     2641 tctccgccgc ccgctccgag ctgtttttgg ccgccgccgc ctccggcgag cgccttacga
     2701 cgctcgttcg tagcgatccc tacggcagca acgccgccca gcacggtgag caccacgagc
     2761 aggatccaaa tcacgtgggg aatatctacg ccgcactgca ccaacgcaat acgtcggcga
     2821 tccttgactc acgggcggcg ggaagtaacg tagcgccgaa atggtcgcag tccccatgca
     2881 agatgatctg cgccgcgtcc tcgacgacga cggcaagcca ttgcccggcg ccaaagtgcc
     2941 ggtcatcccc gatgcgacgc tgcagcgcat cttcgacacc atgatgctgg tccgcatcat
     3001 ggatgaccga atgatgcgtc tccaacgtca gggtcgcctc ggcttctaca tgaagtcgat
     3061 cggcgaagaa gccagccact tcgcggtggc gccgctgcgc gacagcgact ggatcttccc
     3121 gagctaccgc gagcagggcg cgtggttctg gcgcggctac aaggtgcagc agttcatcga
     3181 tcagatgttc ggtaacgtcg atgacccgat caagggccgt cagatgccgg tgcatcactc
     3241 ggcgaactgg ctcaacctgg tctcgatctc gagcccggtc ggaacccaga tcccgcaagc
     3301 ggttggcgcg gcgtatgcgg cgaaggtgtc cggcaaggac gatgtcgcga tggtgtactt
     3361 cggcgaaggc acgagctcga cgggcgagtt tcacgtcggc atgaacttcg cgggcgtctg
     3421 gaaggcgccg tgcgtgttca tctgccgcaa caacggctgg gcgatcagcg tgccgcgcga
     3481 gaagcagacc gcggccaaga cgttcgcggc gaaggccgtc ggctacggca tgcccggcat
     3541 tcgcgtcgat ggcaacgatc tactcgcgat ctggcaggtc gcgagcgaag cgatcgagcg
     3601 cgcacgcgcg ggcgaaggtc cgacgctgat cgaagcgctg acgtatcgcg tgcagggtca
     3661 ctcgagctcg gacgacccga gcgtgtatcg cgatccgaag gagccggaga tctgggagaa
     3721 gaaggatccg atcaaccggc tgcgcgcata catgcgccat cgcggcctgt ggagcgagag
     3781 ctgggagaaa gagctctccg agaagtacaa ccaagagatc accgacgcgc tcgccgcggc
     3841 ggatcacaag ggtgcgcccg cggtcgagac gatgttcgac gacgtctacg aagagatgcc
     3901 gtggaacctg cgcgagcagc gcgagtggct catgcaccag gcccgcacca agaacccgca
     3961 ccaccacgga taaagcacga tgcccgtcat gaacatcatc gaagccgtcc gcaacgcgct
     4021 gcagctgcag atgcgccagg accagcgagt cgtcgtgctc ggcgaagacg tcggcaagtt
     4081 cggcggcgtg ttccgcgcga ccagcggcct gtacgacgag ttcggcgccg accgcgtcat
     4141 cgacacgccg ctcgccgaag ccggcatcat cggcaccgcg atcggcatgg cgctctacgg
     4201 cctgcgcccg gtccccgaga tccagttcgg cgacttcatc ttcccggcgt tcgatcagat
     4261 cgtgaacgag ctggcgaagt ttcggtatcg ctccggcggc cagtacccgt gccccgtcgt
     4321 gattcgcacg cccgtcggcg gcggcatccg cggcggccac tatcactcgc agtcgcccga
     4381 gtcgctgttc atccacacgc ccggcctcaa ggtcgtcgcc ccgagcaatc cgtacgacgc
     4441 gaagggcctg ctcctcgcgt gcatgcgcca gaacgaccct gtgttgttca tggagcccaa
     4501 gcgcatctac cgcgcgagca agggcgaggt gccggagagc gattacgtga tcgagctcgg
     4561 caaggccgcg gttacgcgtg agggtaacca gatcacgctg atcgcgtgga gcggcatggt
     4621 gtcgatcgcc gaggaggccg cgaagaaggc ggccgaggcg ggcatgtccg tcgaggtcat
     4681 cgacatgcgc acgctgatgc cgttcgacat cgacacgatt ctcgagagcg tgaagaagac
     4741 cggtcgctgc gtgatcgtgc acgaagcacc gcgcacctgc ggcttcggtg ccgagctcat
     4801 cgcatcgatc caggaacgtg cgctcgactc cctcgaggcg ccgatcgtgc gcgtcaccgg
     4861 cttcgatact ccgttcccct actcgctcga gcacgagtac atgccgaatg cggaccgcgt
     4921 gctcggcgcg cttcgccaga cgctggagtg gtagccatgg cgttcgactt ctacatgccg
     4981 gatatcggcg aaggtgttgt cgagggcgag atcgtcggct ggaaggtgaa ggaaggcgat
     5041 cgcgtcaagc tcgaccaacc gatcgtcgag gtgatgacgg acaaggcgac ggtcgagctg
     5101 ccgtcgccgc gcgccggcgt catcaagaag atcaattaca aggacggcga gatttgcccg
     5161 gtgggcaaag tgctcgtcgt catcgatgaa gaaggcgcca gcgctgccgc gacgcctgcg
     5221 gccggcggca acggcaagaa gcagccggcg atgatcgtcg aggagccgcc acgcaaggcg
     5281 ccgacgaccg cgcaggtgat gcacagcaag gtcgcgcaga acgtcccgct gacgagcccg
     5341 acgtcctcga gcgcaagcag cagcggcgca atccaggttg tcgatgcgac ggcggatcgc
     5401 gagcgcgtgc tcgccacgcc cgcgacgcgt cgactcgccc gccagctcgg cgtcgaaatc
     5461 ggccgcgtgc ccgcgaccgg caagcacggc cgcgtcacca ccgacgatgt aaagcggttc
     5521 aaggaagctc cgtctggagc gccagccgcc caggcacgca cggcctacac gccggtgtcg
     5581 atcgcgaagg gcggcgacga agagcgcatc ccgctgcgcg gcatccgaaa gaagatcgcc
     5641 gagtcgatga cgcggtcggt gcagaccgcg gcgcacttca cgtacgtcga ggaggtcgac
     5701 atgaccgagc tcgtcgccgt gcgtgaccgc gcgaagtcgc gcgccaccga gcgtggcgtc
     5761 aagctcaact acctgccgtt catcgtgaag gcagtggtgt cgggcctcaa gaagtggccg
     5821 atgctcaacg cgagcctcga cgagagcacg caggagatcg tccgcaagaa gtactaccac
     5881 atcgggatcg cggctcaggg cccacagggc ctcgcggtca gcgtcgtgcg cgatgccgac
     5941 aagcgttcga tcttcgatct gtcgaaggag atcgagcggc tcggcgaagc ggtccggacc
     6001 ggcaccgcta cccgagagga gctaaccggc tcgacgttca cgatcagctc gctcggcaag
     6061 ctgggcggag tgcttgctac cccaattatt aacttccccg aggtcgccat catcggcgtc
     6121 cacaagatcg aggaacggcc ggcggtccga aacggccaga tcgtggcgcg ccacttgatg
     6181 aatctgtcga tatcggtgga tcaccgactc gcggatgggt gggatggcgc aatgttcctc
     6241 caggacgtta aagcgctcct cgaagatccc accacgatgt tcatggagat ggtctaaagg
     6301 ccacggtata gtcccaaggt agtgacgaag aagggttcgg ggacccgcga tagccgttcg
     6361 gagcccgact cggagggcgc gagcgagaat tttcgcgttg atcgcgtgtt cgcccccacg
     6421 cgaccggcct cgcagtcgcg gccgccgatg tcgcggcccg tgacgaagcc gccgacgatg
     6481 cctccgtcgc cgatgggcac gaccccgcca gcgacgatcg ctgcgggcga ctccgagacc
     6541 gagcttgcat tgcggcgcca gctatcgcgc ctgcagcgcc agctcgccga tgcgcaacgc
     6601 gagctcgcga acaaggacga ggaagtcgct gcggcggtcg agaagcggat cgagatccag
     6661 caggcgtatg acgtgatcct cgatgagcag cgccacacgc gaacgctcgt cgacgaagcg
     6721 attgccgatc gcagccgcac gaccggtgtc gagcaacgct tgcaagaagc gcttgctgct
     6781 gccgacgagc tacgccatca gctcgaacgc gaacgtaccg agcgcgcgac gctcgcggtg
     6841 cagttcgacg aagcgaacgc cgccttcgag aagtcgcgca cggcgtggcg cgaggaaagc
     6901 gcgacgatcg acgagcagca catcgcgcaa atcgcacagc tcgaacagca gaagcgcgcg
     6961 gctgtcgacg cggcggaagc tgcaatgaag acttcgaccg agcgccagtt ccaggcccac
     7021 gaggccgagc tcgatgcgct gcgcgcgtcg catcaacgcg agctcgcggc gctacgcggt
     7081 gaaatcgagc cgaaggtgtc cgaagcgcgc aacctgagcg cggagatcga gcggctgtcg
     7141 gcgcagctgc aggcgcaagc cgccgaacat cagagcctgc tcgccgagcg gatcgagttg
     7201 cagacgtggg aattgcagca gcaggccgaa gcaaacgcgg ccgacctcgc ggcactgcag
     7261 cgccagcacg ccaccgaggt ggcgcgactg aacgaagaag tggcggcggc caaccaagcc
     7321 ggccagttga tcga
//
LOCUS       DASUFX010000059        11646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1398565, whole genome shotgun sequence.
ACCESSION   DASUFX010000059 DASUFX010000000
VERSION     DASUFX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398565"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..810)
                     /locus_tag="VFV99_02170"
     CDS             complement(34..810)
                     /locus_tag="VFV99_02170"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX5058131.1"
                     /translation="MSKRTFRVWRGDNKGGEFRTFEVEIEPGMVVLDALHRIQAKDAN
                     DLALRWNCKAGKCGSCSMEINGKPKLSCMTRMNSFGPEETITVQPLKTFPVIKDLVTD
                     VSWNYQQNKRIKPFTPPKPRPEKGYVMYQEDVDRVQEFRKCIECYLCQDTCHVLRDRD
                     GKQEFVGPRFLIRLASLEMHPIDTEDRIPEIRKEYGSGMCNITRCCTEVCPEHIGITD
                     NGIIPLKERVVDRFYDPVAMIGRKLFGIRKRTKTKAEEGK"
     gene            complement(807..2651)
                     /locus_tag="VFV99_02175"
     CDS             complement(807..2651)
                     /locus_tag="VFV99_02175"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX5058132.1"
                     /translation="MTLDASELQTINHDVLIIGAGGAGLRAAIECSALKLSTGVISKS
                     LLGKAHTVMAEGGMAAAMGNVDPRDNWKVHFRDTMRGGKFLNVWRMAELHAKQAPERV
                     RELEEWGAVFDRTKEGLISQRNFGGHRYPRLAHVGDRTGLELIRSLQDHGIHQGIDFH
                     MECTALELLKDGDRIAGVLGYWRETGRFVVFKCKAVILATGGGGKAWKITSNSWEYTG
                     DGLAMAYDAGAEIMDIEFTQFHPTGMVWPPSVRGTLVTEGVRGDGGILKNSKGERFMF
                     NYIPAKFAAETADTVEEAEKWLNGDKNARRPPELLTRDEVARSINAEVKAGRGSPHGG
                     VFLDIASRRPADFIKRKLPSMYHQFKELAEVDITTDPMEVGPTLHYFMGGVRVDADSQ
                     MSTVPGLFACGECAAGLHGANRLGGNSLSDLIVFGKLAGDGAAAYVRGLSSTPQAPND
                     QILAGVRRATDVLRRDSGTNPYVLHDKLTETMQANVGIIREKAELEKALEDLEGLKKE
                     AETMKAPGASQYNPGWHEALSMKSLLITSEAVARAALMREESRGAHTRSDFPGENEEW
                     VQYNVIVKKGANGMEVEKRKRPDPPPMLKEIAYSKIEDLESGKVGADA"
     gene            complement(2656..3498)
                     /locus_tag="VFV99_02180"
     CDS             complement(2656..3498)
                     /locus_tag="VFV99_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058133.1"
                     /translation="MATHLPVIGGHEDGFGKTHRTDTWWVAPAITFFVFSSFIVYTTW
                     ALLQAKYYYSEPYLSPYYSPVLFTDSTAHGAAPEHLAWFGEWPTWWPGHGKWWFLPAS
                     PALLILVFPLSFRMSCYYYRKAYYRAFAGSPPGCAVGPLATGKKKYKGETSLLLIQNL
                     HRFALYFALIFIFLLGKDAFESYFKAGQFGVGVGSIILTINVVLIACYTFGCHSLRHL
                     LGGRKDCMSCGKNTIAYSSWKQATWFNARHAQFAWASLLWVMVTDVYVRLCSMHIIHD
                     FNTW"
     gene            complement(3591..8513)
                     /locus_tag="VFV99_02185"
     CDS             complement(3591..8513)
                     /locus_tag="VFV99_02185"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058134.1"
                     /translation="MDRDGGSGPRTELDLDAAAAAAVEAARDKTTSDTPAAQPADDAV
                     VVDPPLPPSRLEAQPRGTRDDVDPLRPDAPRPARGSSSPARDELPKQARGTRDDLPPP
                     TADRLFAKPADPPAKDKLFATRDDLPAPPPDKPFATPPGDFSFEATAPASRRPDFSLP
                     ERAAETKRSDSTAHEATGSSKFVDFERDATREAPLQTKHVDFERDATREAPLPSKHVD
                     FERDATGEAPLPAKHVDFERDATGEAPLPGNHVHFERDATAESRLPAKPSGQSKPPKR
                     DTAAITAAWLAAKPVVLEGEDTTDEMPVEAKPDVPAKHVDFERDATGEAPLPTHTPPP
                     FLRDKATELDLRIAAAAAVEAARKDLDAAQGAAIDAENAKQEHKTHPGAPSTMGVPTP
                     KKPRPNAFAPTAALAELTADTPPGAELEPLEELPEGTLLAGRYHITELVGEGGMGAVY
                     AAHDEDLDEDIALKVLRPELGRDADFHRRLKAEVRLARRVSHPNVCRVHDIGIDDELV
                     FVTMELVRGNTLRERLAETIAGRAAPLDLAQIVDIVVQIGSALSAAHRAGVIHRDVKP
                     DNVILAGGRAVLTDFGVASMVVDKKHAIVGTPAYLAPEVLRHEPFDHRVDVYAVACLA
                     YELISGTPPFAVRTIEAALTFARDRPSYPGLPDTFATAAVRASLDRVFVRGLASDPHM
                     RPPTMERFTDGFAHAARGAPLSLAVPGRRQERLSTQESVTPVPLVRRTEMRVTTAFVW
                     YANRGPKDTEEAERLVVDAGGSPIRVLPGEIVALFGAMRSTGDDADRAAHTAIRIVDK
                     YGGRAGLDTTRILLRPNENELAGPDSAAAAATLAHSAPEGQVWISKSTARQLAARFAI
                     EAMFGDTRRLMGPREAVDEVAIESMRAQELAAIDAHLERAFRERTPVFVDVRGSAGSG
                     KTRLRKAVMTAVNARRDVEWLIAVTSPVGEPAPLSIVQSCSTEWFDATTKGDASERNA
                     NARRWLETRATHRPIVVVAEDLQWADPTSRALIDFLRVSLERVPVAVLTFSRDEQAGP
                     PPAGVEVVTLAPLADDVATRVARKIAPQASADAIATIVQRAAGNPFFIEELAREAASG
                     STALPTSIEAVVQAHLDQLPATANQVASAAAVIGQGFWRGAVARLMPGFKETELDAAF
                     GDLERAGVIAPAAQAIVADDHYQFTSTLVRDVAYARLPARDRRGQHGLVAGWLDEQTR
                     NSEQDAPRLLAVAHHRENAGDAGRAAVAYKQAGKRCLDLFAYGEATTALRKAAALITV
                     PDPVLDEALADSLTHTEGYAAAEPIYQRALDECDDDDHPSRARLIYKLGAAASKRGNT
                     QNAIARFKAGLAIAAPGDKLAAWAVPDPRTPALLWASLGWTLGYQLGNIDEGHPYCER
                     AVALLEKTPHKRDLAHVLSRLGGTYMRACRFSDQLRCNQRNLEIAVELGDLDMQLTAN
                     INLGVVYGVLGEVQQAVNVTLAARKLATRMSAAASDGLACSNLAGYYLELGRLDEADA
                     ILDEGMAILERTGSRYVLCESLVFRARIAAARGKLADARKHAEESLALARQLGNQLDA
                     AIATRVLAALDSRAGDHDAARVRIEEALVLSATHDEFEGIRTRAARARILALAADPSA
                     ESELDDLQWELSRLGTKRDLAVLRDLTEVR"
     gene            complement(8561..9991)
                     /locus_tag="VFV99_02190"
     CDS             complement(8561..9991)
                     /locus_tag="VFV99_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5058135.1"
                     /translation="MSVTEATAKWVEEQFAKSIVPTLVEYIKIPNKSAMFDPDWKKNG
                     HVDRAVDLLAGWAKTNLPEGATIEVVRLKDGDKERTPVIFIDIPGTGGRQGDTVMLYG
                     HLDKQPEMTGWREGLGPWTPVIEGDKLYGRGGADDGYAIFASLTAINAVRRDKLPHAR
                     CVVLIEACEESGSYDLPAYINHLAPRIGDLSLVVCLDSGCANYDQLWSTTSLRGLVIG
                     NLEVSLLTEGVHSGDGTGVIAASERVVRQLLDRIDDSATGKVKLAALETQIPQQRIKQ
                     AERTAQVIGNEVFDKFPLQPGVKPVASDNVELILNRTWRPGLAITGADGWPPIGNAGN
                     VLRPFTRIKLSIRIPPRVDPKAAAAAVKNALEKDPPYGATVSFTDIGANAGWDAPEIA
                     PWLDKALDEASQQYFGKPAMYMGEGGTIPFMYMLGEKFPKAQFCITGVLGPGSNAHGP
                     NEFLHIPTARKLTLCVAEVLATHAKQ"
     gene            complement(10087..10371)
                     /locus_tag="VFV99_02195"
     CDS             complement(10087..10371)
                     /locus_tag="VFV99_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058136.1"
                     /translation="MDGWDRILERQARQLKTLGIALIAFGVISGASSIYIIATGSDLV
                     YRVYGLAGFVVFLGSGVEAIRRARGVERQLEEKRRARELPVAIARERRDK"
     gene            10612..11589
                     /locus_tag="VFV99_02200"
     CDS             10612..11589
                     /locus_tag="VFV99_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058137.1"
                     /translation="MSACADATPAPAPDSAVPVPIDPTGRYAVTSSFALSSPPAAAAD
                     VLGELLAATDGPDDPSRYLIDLMIERLPPGTTKAYAAALAPYVAAYVNQRLAMVAPHF
                     VDGARALSTGLARIATRFGTTETFDIASDGPRVEGDDYVAESHTLTRTITGVRFDLHG
                     GRDVADVRFAPLGLPDITTGSVAMIDGDRLTIVLHATSLPYTRMLRLGIDFAVIPDVV
                     PAAHDLAQALAVLVDCDQLGAAVAEYMGIGSTSFYATACTLGLTALASRIYARIDAID
                     AASLSLEVAGDAVAVDKTGDGPMDAFMHGTWTGSFASMAVTSSFEGTRQ"
BASE COUNT         1750 a         3830 c         4001 g         2065 t
ORIGIN      
        1 gaacgttcga cgaccgtgcg acaggcttcg aggctatttg ccttcttctg ccttcgtctt
       61 ggtccgctta cggattccaa acaacttacg gccgatcatc gcgaccgggt cgtagaaccg
      121 gtcgaccacg cgctccttga gcggaatgat gccgttgtcc gtgatgccga tgtgctccgg
      181 acacacctcg gtgcagcacc gcgtgatgtt gcacatgccc gagccgtact ccttacggat
      241 ctcggggatg cggtcctcgg tgtcgatcgg gtgcatctcg aggctcgcga gccggatcaa
      301 gaagcgtggg ccgacgaact cctgcttgcc gtcacgatcg cggagtacgt ggcaggtgtc
      361 ctggcacaag tagcattcga tgcacttgcg gaactcctgc acgcgatcga cgtcctcctg
      421 gtacatcacg tagcccttct cgggccgcgg cttcggaggc gtgaacggct tgatgcgctt
      481 gttctgctgg tagttccagg acacgtcggt gacgaggtcc ttgatcaccg ggaacgtctt
      541 gagcggctgg accgtgatgg tctcctccgg cccgaagctg ttcatgcgcg tcatacacga
      601 cagcttcggc ttgccgttga tctccatcga gcagctgccg cacttgccgg ccttgcagtt
      661 ccagcgcagc gcgaggtcgt tcgcgtcctt cgcctgaatg cgatggagcg cgtcgagcac
      721 gaccatgccg ggctcgatct cgacttcaaa tgtacggaac tcgccgccct tgttgtcccc
      781 gcgccagaca cggaaggttc gtttgctcat gcgtcggctc ctaccttgcc ggattcgagg
      841 tcctcgatct tcgagtacgc gatctccttg agcatcggcg gcgggtcggg gcgcttgcgc
      901 ttctcgacct ccatgccgtt cgcacccttc ttgacgatca cgttgtactg cacccactcc
      961 tcgttctcgc cggggaagtc gctgcgcgtg tgtgcgccgc ggctctcttc gcgcatcagc
     1021 gctgcgcgag cgacggcctc gctggtgatg agcagcgact tcatcgatag cgcttcgtgc
     1081 cagcccgggt tgtactggct ggcgccgggc gccttcatcg tctcggcttc cttcttgagc
     1141 ccctcgagat cctcgagtgc cttctcgagc tcggccttct cgcggatgat gccgacgttc
     1201 gcctgcatcg tctcggtgag cttgtcgtgc agcacgtacg ggttcgtgcc cgagtcacgg
     1261 cgcagcacgt cggtcgcgcg gcgcacgccg gcgaggatct gatcgttcgg cgcttgcggc
     1321 gtcgacgaca agccgcgcac gtatgcagct gcgccgtctc cggcgagctt gccgaacacg
     1381 atcaggtcgg acagcgagtt accgccgagg cggttcgcgc cgtgcaggcc agctgcgcat
     1441 tcgccgcacg cgaacaggcc gggcaccgtc gacatctgcg agtcggcatc gacgcgtacg
     1501 ccgcccatga agtagtgcag cgtcgggccg acctccatcg gatcggtcgt gatgtcgact
     1561 tcggccagct ccttgaactg gtgatacatc gacggcagct tgcgcttgat gaagtcggcc
     1621 gggcggcgcg atgcgatgtc gaggaacacg ccgccgtgcg gcgagccacg gcccgccttc
     1681 acttcggcgt tgatcgagcg cgcaacctcg tcgcgggtga gcagctcggg cggccggcgc
     1741 gcgttcttgt cgccgttgag ccacttctcg gcttcctcga cggtgtccgc ggtctcggcc
     1801 gcgaatttcg ccgggatgta attgaacatg aagcgctcgc ccttggagtt cttcaagatg
     1861 ccgccgtcgc cgcgtacgcc ttcggtgacg agcgtgccgc gcaccgacgg aggccacacc
     1921 atgccggtgg ggtggaactg cgtgaactcg atgtccatga tctcggcgcc ggcgtcgtac
     1981 gccatcgcga ggccgtcgcc cgtgtactcc caggagttgc tcgtgatctt ccacgccttg
     2041 ccgccaccgc cggtggcgag gatcacggcc ttgcacttga acacgacgaa gcggccggtc
     2101 tcgcgccagt agccgagcac gccggcgatc cgatcgccgt ccttgagcag ctcgagcgcg
     2161 gtgcactcca tgtggaagtc gatgccctga tggatgccgt gatcctgcag cgagcggatc
     2221 agctcgaggc cggtgcgatc gccgacgtgc gcgaggcgcg gatagcgatg gccgccgaag
     2281 ttgcgctgcg agatcaggcc ttccttcgtg cgatcgaaga ccgcgcccca ttcctcgagc
     2341 tcgcgcacgc gctccggtgc ctgcttcgcg tgcagctcgg ccatgcgcca gacgttgagg
     2401 aacttgccgc cgcgcatcgt gtcgcggaag tggaccttcc agttgtcgcg tggatcgaca
     2461 ttgcccatcg cagcggccat accgccctcg gccatcacgg tgtgagcctt gccgagcaag
     2521 ctcttcgaga tcacgccggt cgacaacttc aaagcgctgc actcgatcgc cgcgcggagg
     2581 cctgcgccac cggcgccgat gatgagcacg tcgtgattga tggtttgcag ctctgatgcg
     2641 tctagggtca tttgactacc aagtgttgaa gtcgtggatg atgtgcatcg agcagagacg
     2701 cacgtaaacg tcggtgacca tgacccagag caagctcgcc cacgcgaact gagcgtggcg
     2761 cgcgttgaac caggtcgcct gcttccacga gctgtacgcg atcgtgttct tgccgcagga
     2821 catgcagtcc ttgcgaccgc cgagcaggtg gcgaagcgag tggcagccga acgtgtagca
     2881 agcgatcaag acgacgttga tcgtgaggat gatgctgccg acaccgacgc cgaattggcc
     2941 tgccttgaag taggactcga acgcgtcctt gccgagcagg aagatgaaga tgagcgcgaa
     3001 gtacagcgcg aaccggtgca ggttctggat gagcaggagc gaggtctcgc ccttgtactt
     3061 cttcttgccc gtcgcgagcg gcccgacggc gcaacccggc ggcgaacctg caaacgcgcg
     3121 gtagtacgcc ttgcggtagt agtagcagct catgcggaac gacagcggga agaccagaat
     3181 gaggagcgcg ggcgaggcgg gcaggaacca ccacttgccg tgacccggcc accacgtcgg
     3241 ccactcgccg aaccacgcga ggtgctcggg ggcagcgccg tgcgcggtgc tgtcggtgaa
     3301 cagcaccggc gagtagtacg gcgagaggta cggctcgctg tagtagtact tcgcctggag
     3361 cagcgcccag gtcgtgtaaa cgatgaacga cgagaacacg aagaacgtga ttgctggggc
     3421 cacccaccac gtgtccgtgc ggtgagtctt gccgaagccg tcctcatgcc ctccgattac
     3481 aggcaggtga gtcgccatgt cgcggcgcac tatagggaaa gcgcccgttt ctaggaaggc
     3541 caaggcgcgt tcatcgcgca gatccgaccc aaaacgcgtt catggtccga tcagcggact
     3601 tccgtgagat cgcgaaggac cgctagatct cgcttggtac cgagtcgcga cagctcccat
     3661 tgcaggtcat cgagctcgga ctcggcgctg gggtcggcgg cgagggcgag gatgcgcgca
     3721 cgggcggcgc gcgtacggat gccctcgaat tcgtcgtgag tcgccgacag cacgagcgcc
     3781 tcctcgatgc gcacgcgcgc agcatcgtga tcgccggcgc ggctgtcgag ggcggcgagc
     3841 acgcgggtcg cgatcgcggc gtcgagctgg ttgccgagct ggcgggcgag cgcgagcgac
     3901 tcctcggcgt gcttgcgggc gtcggcgagc ttgccgcgag cggccgcgat gcgcgcgcga
     3961 aatacgagac tttcgcagag cacgtagcgg ctgccggtgc gttcgaggat ggccatgccc
     4021 tcgtcgagga tcgcgtcagc ctcgtcgagc cggccgagct cgaggtagta gccggcgagg
     4081 ttcgagcacg cgaggccgtc gctcgcggcg gcgctcatcc gcgtggcgag cttgcgggcg
     4141 gcgagcgtga cgttgacggc ctgctggacc tcgccgagca cgccgtagac gacgccgagg
     4201 ttgatgtttg cggtgagttg catgtcgaga tcgccgagct cgaccgcgat ctcgaggttg
     4261 cgctggttgc agcgcagctg atccgagaac cggcacgcgc gcatgtacgt gccgccgagc
     4321 cgagacagca cgtgcgcgag atcgcgcttg tgcggcgtct tctcgagcag tgcgacagcg
     4381 cgctcgcagt aggggtggcc ctcgtcgatg ttgccgagct gatagccgag cgtccacccc
     4441 aggctcgccc acaagagggc cggcgtccgc ggatccggta cggcccacgc ggcgagcttg
     4501 tcgccgggcg cggcgatcgc gaggcccgcc ttgaagcgcg cgatcgcgtt ctgcgtgttg
     4561 ccgcgtttgc ttgcggcagc gcccagcttg tagatgaggc gtgcacggga cgggtgatcg
     4621 tcgtcgtcgc actcgtcgag cgcgcgctga tagatgggct cggctgcggc atagccctcg
     4681 gtgtgtgtca gcgaatcggc gagcgcttcg tcgagtaccg gatcgggcac ggtgatcagc
     4741 gcggcggcct tgcgcagcgc ggtcgtcgct tcgccgtagg cgaacaggtc caggcagcgc
     4801 ttgccagctt gcttgtatgc gacggctgcg cggcctgcat cgccggcatt ctcgcgatgg
     4861 tgagcgaccg cgagtagacg cggcgcatct tgctcggagt tgcgcgtctg ttcgtcgagc
     4921 cagccggcga caaggccgtg ttgtccgcgg cggtcgcgcg ccggaaggcg ggcgtacgcg
     4981 acgtcgcgca cgagcgtcga cgtgaactgg tagtgatcgt cggcgacgat agcctgcgcc
     5041 gcgggcgcga tgacgcctgc gcgttcgaga tcgccgaacg cggcgtcgag ctcggtctcc
     5101 ttgaaccccg gcatgaggcg cgcgaccgca ccgcgccaga agccctgacc gatgaccgcg
     5161 gcggcactcg cgacctgatt cgccgtcgcg ggcaactgat cgaggtgggc ctggacgacc
     5221 gcttcgatcg acgtcggtag cgcagtgcta ccgctcgcag cctcgcgcgc gagctcctcg
     5281 atgaagaacg gattgccggc ggcgcgctgg acgatggtcg cgatcgcgtc cgcgctcgcc
     5341 tgcggcgcga tcttgcgtgc gacgcgggtg gcgacgtcgt cggccagagg tgcgagcgtc
     5401 acgacctcga cgcccgccgg tggtggtccc gcctgctcgt cgcgcgagaa cgtgagcacg
     5461 gcgaccggaa cgcgctcgag gctgacgcgc aagaagtcga tcagcgcgcg agacgtcggg
     5521 tccgcccact ggagatcctc ggcgacgacg acgatcggcc ggtgggtcgc gcgcgtctcg
     5581 agccagcgcc gcgcgttggc gttgcgctcg ctggcgtctc ccttcgtggt cgcatcgaac
     5641 cactcggtgc tgcagctctg cacgatcgag agcggcgcgg gctcaccgac cggcgacgtc
     5701 accgcgatca gccactcgac gtcgcggcgc gcgttgaccg cggtcatcac ggccttgcgc
     5761 aggcgcgtct tgcccgagcc cgcgctacca cgcacgtcga caaacaccgg cgtgcgctcg
     5821 cggaatgcgc gctcgaggtg cgcgtcgatc gcagcgagct cttgcgcgcg catcgattcg
     5881 atcgcgactt cgtcgacggc ttcgcgcggc cccatgaggc ggcgcgtgtc gccgaacatc
     5941 gcttcgatcg cgaagcgtgc ggcgagctgg cgcgcggtgc tcttgctgat ccagacctga
     6001 ccttcgggcg cgctatgcgc cagcgtcgcc gctgccgccg ccgagtcggg accggcgagc
     6061 tcgttctcgt tcgggcgcag caagatgcgc gtcgtatcga gaccagcgcg accgccgtac
     6121 ttgtcgacga tccggatcgc cgtgtgcgct gcgcgatcgg catcgtcacc cgtcgagcgc
     6181 atggcaccga acagcgcgac gatctcaccc ggcagcacgc ggatcggcga gccaccggca
     6241 tcgacgacga ggcgctctgc ctcttcggta tctttcgggc cgcggttcgc gtaccagacg
     6301 aacgcggtcg tcacgcgcat ctcggtgcgc cgaacgagcg gcaccggcgt caccgactct
     6361 tgcgtcgaca gccgttcctg gcggcggccc ggtacggcga gcgagagtgg ggcgccgcgt
     6421 gccgcgtgcg cgaacccgtc ggtgaagcgc tccatcgttg gcggtcgcat gtgcggatca
     6481 gacgcgaggc cgcggacgaa cacgcgatcg agcgatgcgc gcacggccgc cgtcgcgaac
     6541 gtatcgggca ggccgggata cgaaggtcga tcgcgtgcga acgtgagcgc cgcttcgatc
     6601 gtacgcaccg cgaacggcgg tgtaccggag atgagctcgt acgcgaggca cgccaccgcg
     6661 tagacgtcga cgcggtggtc gaacggttcg tgccgcagca cctcgggcgc gagatacgca
     6721 ggcgtgccga cgatcgcgtg cttcttgtcg acgaccatcg acgcgacgcc gaagtcggtg
     6781 agcacggcgc ggccgccagc gaggatcacg ttgtcgggtt tgacgtcgcg gtggatcacg
     6841 ccggcccggt gcgccgcaga cagcgccgac ccgatctgga cgacgatgtc gacgatctgc
     6901 gcgaggtcga gcggcgccgc gcggccggcg atcgtttcgg ccagtcgctc gcgcagcgta
     6961 ttgccgcgca cgagctccat cgtgacgaac acgagctcgt cgtcgatgcc gatgtcatgg
     7021 acgcgacaca cgttcggatg cgagacgcga cgggcgaggc ggacctcggc cttcagccgg
     7081 cggtggaagt cggcgtcacg gccgagctcg gggcgcagca ccttgagcgc gatgtcttcg
     7141 tcgagatcct cgtcgtgcgc ggcgtagacc gcgcccatgc cgccttcgcc gacgagctcg
     7201 gtgatgtgat agcggcccgc gagcagcgtg ccctcgggca gctcttcgag tggctcgagc
     7261 tccgcgccgg gcggcgtgtc cgccgtgagc tcggccagcg cggcagtcgg cgcgaacgca
     7321 ttcggtcgcg gcttcttcgg cgtcggtacg cccatcgtgc tcggcgcgcc gggatgcgtc
     7381 ttgtgctctt gcttcgcgtt ctcggcgtcg atcgcggctc cttgcgctgc atcgaggtcc
     7441 ttgcgcgcgg cttcgaccgc ggctgccgct gcgatgcgca gatcgagctc ggtcgcttta
     7501 tcgcgcagga acggcggcgg agtatgggtc ggcagtggtg cctcaccggt cgcgtcgcgc
     7561 tcgaagtcga cgtgctttgc gggcacgtcg ggcttggcct ccaccggcat ctcgtcggtg
     7621 gtgtcttcgc cctcgaggac gacgggcttg gccgccaacc acgcggcagt gatggcagca
     7681 gtgtcgcgct tgggaggttt ggattgcccg ctgggcttgg caggcaaccg cgattcggcg
     7741 gtggcgtcgc gttcgaaatg gacatgattg ccaggcagcg gcgcctcacc cgtcgcgtcg
     7801 cgttcgaagt cgacgtgttt agcaggcagc ggtgcttcac cggtcgcgtc gcgttcgaag
     7861 tcgacgtgct tgctcggcag cggtgcctcg cgggtcgcgt cgcgctcgaa gtcgacgtgc
     7921 ttcgtctgca acggtgcttc acgggtcgcg tcgcgctcga agtcgacgaa cttgctcgat
     7981 ccggtggcct cgtgcgccgt cgagtcggag cgcttcgtct ctgcggcgcg ctcgggaagc
     8041 gagaagtcgg ggcggcgcga tgcgggcgcg gtcgcttcga agctgaagtc gcctggtggt
     8101 gttgcgaacg gcttgtccgg aggtggcgcc ggtagatcgt cgcgcgtcgc gaacagcttg
     8161 tccttcgcag gcggatcggc cggctttgcg aacaaccgat ccgcagtcgg cggcggcaga
     8221 tcatcacgcg tgccgcgcgc ttgtttcggg agctcatcgc gcgccggtga cgagctgccg
     8281 cgtgcgggtc gtggtgcgtc cggacgcaga ggatcgacgt cgtcgcgtgt accgcgcggc
     8341 tgcgcttcga gccgactagg cggcagtggc ggatcgacta cgactgcatc gtccgcaggt
     8401 tgcgcggccg gcgtgtccga ggtggtcttg tcccgcgcag cctcgacggc tgcggcagcg
     8461 gccgcatcga gatccagctc ggtgcgcggc ccggaaccac catcccggtc catcagccac
     8521 tatcgtaacc gattccgcaa tctcggcgag tgtcagtggg ttactgcttg gcgtgggtcg
     8581 cgaggacctc cgccacgcac agtgtgagct tgcgcgccgt cggaatgtgc aggaactcat
     8641 tcggtccgtg cgcgttcgag ccgggaccga gcacgccggt gatgcagaac tgcgctttcg
     8701 ggaacttttc accgagcatg tacatgaacg ggatcgtgcc gccttcgccc atgtacatcg
     8761 cgggcttgcc gaagtactgc tgcgacgcct cgtcgagcgc cttgtcgagc cagggtgcta
     8821 tttcgggcgc atcccagccg gcattggcgc cgatgtcggt aaacgagact gtcgcgccgt
     8881 acggcggatc tttctcgagc gcgttcttca ccgcagcggc cgccgccttc ggatcgacgc
     8941 gcggcgggat gcggatcgac aacttgatcc gcgtgaacgg gcgcagcacg ttgccggcgt
     9001 tgccgatcgg cggccagcca tcagcgccgg tgatcgcgag gcccggacgc cacgtgcggt
     9061 tcaagatcag ctcgacgttg tcgctggcga ccggtttcac tccgggctga agcgggaact
     9121 tgtcgaacac ctcgttgccg atcacctgcg cggtgcgctc cgcctgcttg atccgctgct
     9181 gcggaatctg cgtctcgagc gcggcgagct tcaccttgcc ggtcgcactg tcatcgatgc
     9241 gatcgagcag ctgacgcacg acgcgttcgc ttgccgcgat cacgccggtg ccgtcgcccg
     9301 agtggacgcc ttcggtgagc agcgagactt cgaggttgcc gatcacgaga ccgcgcaacg
     9361 acgtcgtcga ccacagctga tcatagttcg cacagccaga gtcgaggcac acgacgagcg
     9421 acaggtcgcc gatgcgcggc gcgagatgat tgatgtacgc gggcaggtcg tagctacccg
     9481 actcctcgca cgcttcgatc agcacgacgc agcgcgcgtg cggaagcttg tcgcggcgca
     9541 ccgcattgat cgcagtgagc gacgcgaaga tcgcgtaacc atcgtcggcg ccaccgcggc
     9601 cgtacagctt gtcgccctcg atgacgggcg tccacggccc gaggccttcg cgccagccgg
     9661 tcatctccgg ctgcttgtcg aggtggccgt agagcatgac ggtgtcgccc tggcggccac
     9721 cggtgcccgg gatgtcgatg aagatgaccg gcgtgcgctc cttgtcgccg tccttgagcc
     9781 ggacgacttc gatcgtcgcg ccttcaggca ggttggtctt cgcccagccg gcgagcagat
     9841 cgaccgcgcg atcgacgtgg ccgttcttct tccagtcggg atcgaacatc gccgacttgt
     9901 tcggaatctt gatgtactcg acgagcgtcg gaacgatcga cttggcgaac tgttcctcga
     9961 cccacttcgc ggtggcttcg gtgaccgaca tgcggcgggc gtaacacgga tccgtcgcgg
    10021 cggcgacggg agcgcgggtg tcgcagcgtg tctcagtcgc acgccgcgtc gaatctcacc
    10081 ggcgcgtcac ttatcgcggc gctcgcgggc gatcgcgacc ggtagctctc gtgcgcggcg
    10141 cttctcttcg agctgacgct cgacaccacg tgctcgccgg atcgcctcga cacccgagcc
    10201 aaggaacacc acgaacccgg cgaggccgta gacccgatac acgaggtccg agccggtcgc
    10261 gatgatgtag atcgacgacg cgccggagat gacgccgaac gcgatgagcg cgatgccgag
    10321 cgtcttcagc tggcgcgctt ggcgctcgag gatgcgatcc cagccgtcca cggcaccagc
    10381 gtatcgcaag tcggttcgcg atcgcggcgt agcgccagcg ccgccatcgt tctctgcgtc
    10441 cgacgcacgg accacgtcgt ccggcaggcg gttcgtcgga cggacagggc gcgtcgtgcg
    10501 cgagtcgaat cgcgtggttc gcgcgacgca cgagcggcat gaccgctgca ctcgtcgccg
    10561 cgcatgtcga accatcgctt ctcctttgca tcgctcctcg tcgttgtctt cgtgtccgct
    10621 tgcgcggacg ccacacccgc acctgcgccg gacagcgcgg tccccgtgcc gatcgatccg
    10681 accggtcgct acgcggtcac cagctcgttc gcgttgtcgt caccacccgc tgccgcggcc
    10741 gacgtgctcg gcgagcttct cgctgcgacg gacggccccg acgatccgag tcgctacttg
    10801 atcgatctga tgatcgagcg gctgccgccc ggcacgacga aggcatacgc cgccgcgctc
    10861 gccccgtatg tcgccgcgta cgtcaaccag cgcctcgcga tggtcgcgcc gcacttcgtc
    10921 gatggcgcgc gtgcactgtc gaccgggctc gcgcggatcg cgacacgctt cgggacgacc
    10981 gagacgttcg atatcgcgag cgacgggccg cgtgtcgaag gcgatgacta cgtcgccgag
    11041 agtcacacgc tgacgcgcac gatcaccggc gttcgcttcg atctgcacgg cgggcgcgat
    11101 gtcgccgacg tccggttcgc gccgctcggc ttgcccgata tcacgacagg gtcggtcgcg
    11161 atgatcgatg gcgatcgact cacgatcgtc ctccacgcga cgtcgttgcc gtacacgcgc
    11221 atgttgcggc tcggcatcga cttcgccgtg attccggacg tcgtgccggc cgcgcacgat
    11281 ctcgcgcaag cgctcgccgt gctcgtcgat tgcgatcagc tcggcgcggc ggtcgccgag
    11341 tatatgggca tcggctcgac gagcttctac gcgaccgcgt gcacgctcgg cctcaccgcg
    11401 ctcgcgtcgc ggatctatgc gcgcatcgat gcgatcgatg ctgcgtcgct gtcgctcgag
    11461 gtcgccggtg acgcggttgc cgtcgacaag accggcgacg ggccgatgga cgcgttcatg
    11521 cacggcacgt ggaccggcag tttcgcgagc atggccgtca cctcgagctt cgaggggacc
    11581 cggcaatgaa aggcgctaca cttcgcatca tggacccgcc accgccgtac gacgttccaa
    11641 cgtaca
//
LOCUS       DASUFX010000060         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_982506,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000060 DASUFX010000000
VERSION     DASUFX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982506"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..754
                     /locus_tag="VFV99_02205"
     CDS             95..754
                     /locus_tag="VFV99_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058138.1"
                     /translation="MTQDDYGTATVPDCNDEGAPFNPSEDWIEAFENQNTEEMRKRAL
                     RFARARARFVSRAGGRVDELYVNELVHDALTDTLLGVLAWDPAAISLETHVFGTIRFR
                     TKNDRIRALRFRHQSIDAADAGGVMCEVEATLASAQSAPDPESLTFSAEVLAMLRNLA
                     GDDAEIHRIVDAIEAGASAPREIMSVGQLSEKTWRKARLRMARIVDQLSNHVLLAVRH
                     A"
     gene            763..1128
                     /locus_tag="VFV99_02210"
     CDS             763..1128
                     /locus_tag="VFV99_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058139.1"
                     /translation="MTKPTTEQILDEVSYQAALAEADHGASTRADDRASRELGDKLQA
                     RLAQLRRNLLPASAPIQKAKPIRTKWLGLDRPALLARITEMTQAMSGAVQYAHRDLQG
                     LSDNDLRQLLDLLDTSPRE"
     gene            1141..1944
                     /locus_tag="VFV99_02215"
     CDS             1141..1944
                     /locus_tag="VFV99_02215"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX5058140.1"
                     /translation="MRDLWIRRARQAARALSRRFGVTAPEHINIEVFAARLGATVTVG
                     PLEGAIAQLVRVGSHVHIVVSERVTDDCAIRFSIAHELGHFVLGHPSRPPSELCGGVR
                     PRAGASDARNYELEANAFAGELLMPSHLLQRWCEVSPVSMEIPRRIAREYNVSILASA
                     IQFAELSSERCAAVFSARREVVWSASSATFEREIPRGRRIDPHSVAWDFYVRGELDDC
                     AQPVPADAWLDTSIEVDIVEHSIASPQHGTVLSLLWIPERSASRLSTHF"
     gene            2001..2237
                     /locus_tag="VFV99_02220"
     CDS             2001..2237
                     /locus_tag="VFV99_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058141.1"
                     /translation="MRKQDSQELLQIDPAHIRHFLSIFNALSPEEAAQVREIAEAFSP
                     AELRAWFAQLVDLNEPQAIQHIKVLVAGNTEAVS"
     gene            2234..2662
                     /locus_tag="VFV99_02225"
     CDS             2234..2662
                     /locus_tag="VFV99_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058142.1"
                     /translation="MNPLPDPFDWSLLFRAFNDALEWVLDVAAHTARNYPDEVATVER
                     VRTFTRARNAGRPAHLRFDDLLFTFSLVLAAIERDLGPLTKRPPTFMPEASWHRRSPS
                     LHGSVRSSRSKSARRIAATSSKSPTGTKMSFVGPAPTSTT"
     gene            2659..2931
                     /locus_tag="VFV99_02230"
     CDS             2659..2931
                     /locus_tag="VFV99_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058143.1"
                     /translation="MNAIVAVFDPILRTQPLRFGGHGLGQRWRSSIDALAHHAGIAEY
                     RDNRTFWRALPAVCLYLHSQSVPLPPPEVWNALLARFRATPAAALA"
     gene            2980..>3384
                     /locus_tag="VFV99_02235"
     CDS             2980..>3384
                     /locus_tag="VFV99_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5058144.1"
                     /translation="MSDDAKPADDAHWFWPLPRLAGESPRIIAHAHDDRLGVDLGYAR
                     IVFPELFVPVFAVRAGLVSFAARCASGYAVTIEHGGIVSSHYAHLEQMFVTPTLGRRR
                     RRLRVRAGDVIGYAGRDPSHIRFEVWKWTAKNG"
BASE COUNT          586 a         1103 c         1085 g          610 t
ORIGIN      
        1 gcccaatcgc cgaatgcgtg cggttttcag tggttactga acacgcggcc agatgtcaga
       61 tcccccctgt agagtcgcta tccaaggagc ccaaatgacc caagacgatt acggcacagc
      121 gactgtgccg gactgcaacg acgaaggggc gccgttcaat cccagcgagg actggatcga
      181 ggcgttcgag aaccagaaca ccgaggagat gcgcaagcgc gcgctccggt tcgcgcgtgc
      241 tcgtgcgcgc tttgtgtcgc gtgccggcgg ccgtgtcgac gagctgtacg tgaacgagct
      301 cgtacacgat gcgttgacgg acacgctgct cggcgtgctc gcgtgggatc cagccgcgat
      361 ttcgttggag acccacgtgt tcggcacgat ccgcttccgc acgaagaatg atcggattcg
      421 cgcgctgcgc tttcgccatc aatcgatcga cgcggcggat gccggcggcg tgatgtgcga
      481 agtcgaagcg acgctcgcgt cggcgcagag tgcgccggac cccgagtcgc tgacgttctc
      541 ggcggaggtg ctcgcgatgc tgcgcaatct cgccggcgat gatgccgaga ttcaccggat
      601 cgtcgacgcg atcgaagcag gcgcgagtgc gccgcgcgag atcatgagcg tcggccagct
      661 gtcagagaag acatggcgca aggcgcggct gcgcatggcg cgcattgtcg atcaactctc
      721 aaaccacgtg ctgctcgcag tacgccacgc atgaggatca ccatgacaaa gccaaccacc
      781 gaacagatcc tcgacgaagt gtcgtatcag gccgcgctcg cggaagctga tcatggcgcg
      841 agtacacgcg ccgacgaccg cgcatcgcgc gagctcggcg acaagctaca agcacgcctc
      901 gcgcagttgc ggcgcaactt gctgccggcc agcgcgccga ttcagaaggc caagccgatt
      961 cgtacgaagt ggctcggcct cgatcgcccg gcgttgctcg cgcggatcac cgaaatgacc
     1021 caggcgatga gcggtgccgt gcagtacgcc catcgcgatc tgcaggggct gagcgacaac
     1081 gatctgcgcc agctgctcga tctgctcgat acgtcaccac gcgaatagcc aggagcatac
     1141 atgcgcgatc tatggatccg cagggcgcgg caggctgccc gcgcgctgtc gcgccgtttc
     1201 ggtgtcactg caccggagca catcaacatc gaggtgtttg cggcgcgtct cggcgcgacg
     1261 gtcaccgtcg gtccgctcga gggcgcgatc gctcagctcg tgcgcgttgg ctcgcacgtg
     1321 cacatcgtgg tctccgagcg cgtgaccgat gactgcgcga tccgcttttc gatcgcgcac
     1381 gagcttggcc atttcgtgct cggtcatccg tcgcgaccac cgtcagagct gtgcggcggc
     1441 gtacggccgc gcgcaggtgc gagcgacgcg cgcaactacg agctcgaggc caatgcgttc
     1501 gcgggtgagc tgctgatgcc aagccatttg ctccagcgct ggtgcgaggt gtcgccggtg
     1561 agtatggaga tcccgcggcg gatcgcgcgc gaatacaacg tgtcgatcct cgcgagcgcg
     1621 atccagttcg cggagctgtc gtcggaacgc tgcgcggccg tgttctcggc gcgacgcgag
     1681 gtggtgtgga gtgcgtcgag cgcgacattc gaacgcgaga ttccgcgtgg cagacgaatc
     1741 gatccgcact cggtcgcgtg ggatttctac gtgcgcggcg agctcgacga ctgcgcgcaa
     1801 ccggtgcctg cagatgcgtg gctcgacacg tcgatcgagg tcgacattgt cgagcattcg
     1861 atcgcgtcgc cgcagcacgg aactgtgctg tcgctgctgt ggatcccgga gagatctgca
     1921 tcacggttga gtacacattt ctgatcaaac acgatccaaa acgccgcgct ggtcgccctt
     1981 accaagaaag gagatcagtg atgcgcaagc aagattccca agagctgctt cagatcgatc
     2041 ccgcacacat ccgccacttc ctgtcgatct tcaacgcgct gtcgcccgag gaggcggcac
     2101 aggttcgtga gatcgcggag gcgttcagcc cggcggagct gcgtgcgtgg ttcgcacagc
     2161 tcgtcgacct caacgagccg caagcgatcc agcacatcaa ggtgctggtt gcgggcaata
     2221 ccgaggcggt ttcgtgaacc cactgcccga tccgttcgat tggtcgctgt tgtttcgtgc
     2281 gttcaacgat gccttggagt gggtgctcga cgtcgccgcg catacggcgc ggaactatcc
     2341 cgatgaagtc gcaaccgtcg agcgcgttcg cacgttcacg cgggcgcgca acgcaggtcg
     2401 ccctgcacat ctgcgtttcg atgatctgtt gttcacgttc tcgctagttc tcgccgcgat
     2461 cgagcgcgac ctgggtccgc tcaccaagcg gccgccgacg ttcatgcccg aggcatcatg
     2521 gcaccgtcgt tccccatcac ttcatggctc cgtgcgttct tcgcgctcca agagtgcgcg
     2581 gcggatagcc gcgacgtcat cgaaatcccc gacggggacg aagatgtcat tcgttggccc
     2641 cgcaccgacg tcgacgacgt gaacgcgatc gtcgcggtct tcgatcccat tcttcgtaca
     2701 caaccccttc ggttcggcgg tcacggcctc gggcagcgct ggcgcagcag tatcgacgcg
     2761 ctcgcgcacc acgcaggcat cgccgagtac cgagacaacc ggacgttctg gcgcgcgctt
     2821 ccggccgtct gtctctatct ccattcgcaa tcagtgccgc tgccgccgcc agaagtctgg
     2881 aacgcgctgc tcgcgcgctt ccgcgccacg ccggctgcgg ctctcgcata ggaactcatg
     2941 ccgttacctc atcagcccac tctcaccaca cgttatctct tgagcgacga cgcgaaaccc
     3001 gccgacgacg cgcactggtt ctggccactg ccgcggctcg ccggcgaatc accgcgcatc
     3061 atcgcgcacg cgcacgatga ccggctcggc gtcgacctcg gctacgcgcg gatcgtgttt
     3121 cccgagctgt tcgtgcccgt gttcgcggtg cgcgccggcc tcgtcagctt cgccgcacgc
     3181 tgtgcgagtg gctacgccgt cacgatcgag cacggcggca tcgtgtcgtc gcactacgcg
     3241 cacctcgagc agatgttcgt cacgccgacg ctcgggcggc ggcgccggag gttgcgcgtg
     3301 cgtgccggcg acgtgatcgg ctacgcgggg cgcgatccca gtcatattcg cttcgaagtg
     3361 tggaagtgga cggcgaagaa cggt
//
LOCUS       DASUFX010000061         7644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_982631,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000061 DASUFX010000000
VERSION     DASUFX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982631"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFV99_02240"
     CDS             <1..729
                     /locus_tag="VFV99_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058145.1"
                     /translation="LIRCGGGGADGVQGVATRGDRIAIAGTFTAGAELGGVALPAYAD
                     DSPFGDGFAAELDGNGKRRWAQTFGGRADDAVAGVAIDDRDHVIVAGSVHEVVHVGSA
                     QLVTQGPGDGIIVWFGDDGEKGAAVLVGGLDFDGLRAITAVGDKAIVAGFFSGSMKLG
                     DRTLTAGGGDDSFIAAFDGNGTIASGWHVGGEGREEVTSLSAIPGGFVAGVAHTAKAS
                     IEDTQLAAPKDPMRGSALIVRGVR"
     gene            complement(726..1967)
                     /locus_tag="VFV99_02245"
     CDS             complement(726..1967)
                     /locus_tag="VFV99_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058146.1"
                     /translation="MKAFASVVLFLTGCAATSSFTSSSSSSRASGADSSGTVTIPNVF
                     KLRKQEAIAALRRAGVQGDINDDGSMCGSFVDGKIIEIGEVCYQFPAAGRVQGARLVV
                     SLRVQTDDPRHGNIGKNTEWYLMPNLIGMTFEQALAEMHRAGFAQDDRVQHVWADETT
                     TCKPNVVCRQYPDVNERAGLNDGKILYFGQERRPTVTGSDTPPPPTTTTATTPPAPTT
                     TSTTTTTTSTTSTPSKPATPPSPPATTTTAANDKHYGGNGSPPYRDSHNAPHGPGGPV
                     YMGRGEPCTNKIDHCLRPGVWFAADNVVAGSLFRGTPVFELEGKWWTWRGDEAEYKFL
                     FKTKVADKVAEVEVGKPVLFFVEEGGRKWLDNEYDMLTSSRWSLGVVDAATADAITIR
                     GWGPVSLESIRVIVEKKERAN"
     gene            complement(2005..2829)
                     /locus_tag="VFV99_02250"
     CDS             complement(2005..2829)
                     /locus_tag="VFV99_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058147.1"
                     /translation="MTLLVGLHCVFHRALDGDQERLARLATLVVDPRWLYRFKLINVW
                     HTPQRPAVPVFKGTVPAAKLRQSVTEAITSTSSQHVLFGVSRSKQDTLDTARFGIDLG
                     RPLTMDVALPFEACSTWHVPADASDEATKVWLDLQHEIVELVGALNGVVIVTTNADIV
                     AIETSLVSRTLNGRPLHPDPGEISSYGIHRRKLGEDYIRVPRWGTYLKPAHVAAVGGR
                     DKIVSVVKPPVVRDVGELLYVQLSERVSDALAPETEARRRAFADLVAPITAPRVNA"
     gene            complement(2826..4385)
                     /locus_tag="VFV99_02255"
     CDS             complement(2826..4385)
                     /locus_tag="VFV99_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058148.1"
                     /translation="MSSKVVVLIAGVVVGGLLVGDQRAHADDDSDRADLIRNIDDRIK
                     SMASELSGFDSDRDNGDLNDALSYAREVRDLVGRLDRVKGSDSRANDIVDKYPRYLDG
                     FDAAAKFLSRMKDLQFLADGVADKCNSDEADLQTVVRYYVGRPDDADEAFKTLPDKAK
                     EYGKTWTERLATLKQNDSDFSSVLYYARFTEDRDEWSRVSSSFNDDASRMASFWKDRY
                     RAVTEGACPRLALGEKHPDVDKGLENLRNYTATTKGTVTQLTKDYNAWLREARQLRAF
                     TEKDRDDLREVMCRAGEYEMEAKVKEVADRWASQISSAYGTMLGQSDRLESRATDSKI
                     AHYKGSKRVVEGIQANRRNFEKLRSYELQGANNPKIRAKLEYGKKVHADRQSSMCSGS
                     SGYAEFKITSTDCRNEVREGSDCRADCVLTGSSCKVIEIKPRTQDAMYEGGRQMKAYT
                     EGLKNWYARDKASLFEKYPQVRNCERDGKELVVDPPDLDIYDFCPDAAEAKGFGDDVA
                     EPPADAPETGE"
     gene            complement(4490..5743)
                     /gene="odhB"
                     /locus_tag="VFV99_02260"
     CDS             complement(4490..5743)
                     /gene="odhB"
                     /locus_tag="VFV99_02260"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5058149.1"
                     /translation="MADLVVPQLGESISEAVVAKWLKQVGDAVADGEPVAELETDKIT
                     VELPSPVAGALTEQRFAVGATVKVGDVIGAVDAGKAGKAAAKPAAAKVEPAKPAPAAA
                     AQPQVAAQPAPVKQPPPPPQAAAQAKPAAKSGNGESFDKDQLLKLTPSQRATARETGA
                     LPARGAAAPAAPSGPSVEDRLAQVDPRDEIVPMSPLRKRVAERLVQAQHESASLTTFN
                     EVDMTAIMELRAKYKDSFEKAHGAKLGFMSFFVKACVAAAKLFPGINAEVVGGNIVYK
                     KHYDFGIAVSTPKGLVVPVLRDCDALSFAGVEKGILALAEKARNGKLGLDDLQGGTFS
                     ITNGGIYGSMMSTPLLNYPQTGILGMHNIVKRPMVVGDEVKVRPMMYVALSYDHRVVD
                     GREAVSFLVAVKDRLEAPDRMLVEV"
     gene            complement(5768..>7644)
                     /locus_tag="VFV99_02265"
     CDS             complement(5768..>7644)
                     /locus_tag="VFV99_02265"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5058150.1"
                     /translation="LAFNPSHLEAVDPVVVGRVRAKQTRHGDVEHKRVVGVLVHGDAA
                     FAGQGLVPETLQLSNLPGYRTGGTVHIIVNNQVGFTATPAEQRSTPYCTDVAKMIECP
                     IWHVNGEDLDALARVVEIACEYRAQFGSDVVIDMYCYRKYGHNENDEPAFTQPIMYDR
                     IKEKQSPVEVYTHKLVADHTITADEATAMTQRRVAELETELEAAKAAKQRPEGASMTA
                     MWRGYRGGITDEPEEVDTRVMRPLLEQIAQSMTEVPQGFTPHPKIVRLLEQRAQMGRG
                     DKPLDWGMGELLTYGSLLYQAVNVRLTGQDVSRGTFSHRHAVITDIVNGREHMLLGQL
                     HPDQGQCRIYDSPLSEAGVMGFEFGYSLDYPDALVMWEAQFGDFANGAQVIIDQFISS
                     SEDKWKRLSGLVLLLPHGYEGQGPEHSSARLERYLQSCAEHNMRVAQPTTPAQVFHLL
                     RMQVLRRLRKPLVVMTPKSLLRLPAATSTLDELATGRFHRVMHDTTADPAHVTRVLFC
                     TGKIYYELVDERSKRKDDTVAIVRLEQLYPWWPHLVADAIAPYTKLRECFWVQDEPHN
                     MGAGRFVTPRIESILHDRGGNLKYEFISRVESASPATGSHKAHVLEQQQILKAAFDPR
                     "
BASE COUNT         1248 a         2510 c         2427 g         1459 t
ORIGIN      
        1 ctcatccgct gcggcggcgg cggtgccgat ggcgtgcaag gcgttgccac gcgcggcgat
       61 cggatcgcga tcgcaggcac gttcacagcg ggcgccgagc tcggcggtgt cgccctgccc
      121 gcctacgcgg acgactcacc gtttggcgat gggtttgcgg ccgagctcga tggcaacggc
      181 aagcgacgct gggcgcagac gttcggcggc cgcgccgacg atgcggtcgc cggtgtcgcg
      241 atcgacgatc gcgatcacgt gatcgtcgcg ggcagcgtcc acgaggtcgt gcacgtcgga
      301 agcgcgcagc tcgtcaccca gggccccggc gacggcatca tcgtgtggtt cggcgacgac
      361 ggcgagaaag gcgcggcggt gctcgtcggc ggtctcgact tcgatggtct gcgcgcgatc
      421 acggcggtcg gcgacaaggc gatcgtcgcc ggcttcttct ctggctcgat gaagctcggc
      481 gatcgtacgc tcaccgcggg cggcggcgac gattcgttca tcgccgcgtt cgatggcaac
      541 ggcacgatcg catccggctg gcacgtcggc ggcgaaggcc gcgaagaggt cacgtcgctg
      601 tcggcgattc ccggtggctt cgtcgccggc gtcgcgcaca ccgcgaaagc gtcgatcgaa
      661 gacacgcagc tcgccgcacc gaaagatccg atgcgcggca gcgctctgat cgtgcgcggc
      721 gtgcgttagt tcgcgcgctc tttcttctcg acgatcacgc ggatcgactc gagcgagacc
      781 gggccccagc cgcgaatcgt gatcgcatcg gcggtcgcag catcgacgac gccgagcgac
      841 cagcgcgacg acgtgagcat gtcgtactcg ttgtcgagcc acttgcggcc accttcttcg
      901 acgaagaaca gtaccggctt cccgacttcg acctccgcga ccttgtccgc gaccttggtc
      961 ttgaacagga acttgtactc ggcttcgtca ccacgccacg tccaccattt accctcgagc
     1021 tcgaacacgg gcgtcccgcg aaacaggctg cccgcgacga cgttgtcagc cgcgaaccac
     1081 acccccggcc gcaagcagtg atcgatcttg ttcgtgcagg gctctcccct ccccatgtac
     1141 accgggccgc cggggccgtg cggcgcgttg tgcgaatcgc gatacggtgg tgaaccgttg
     1201 ccaccgtagt gcttgtcgtt cgcggctgtt gtcgtcgtcg ctggcggtga cgggggggtc
     1261 gcaggcttcg acggtgtcga agtcgtcgag gtcgtcgtgg tcgtcgtgct cgtcgtcgtc
     1321 ggcgccggcg gcgtcgtcgc cgtcgtcgtc gtcggcggcg gcggcgtatc ggagccggtg
     1381 accgtcggcc ttcgctcctg cccgaaataa aggatcttgc cgtcgttgag tccggcccgc
     1441 tcgttcacgt ctggatactg acgacacacg acgttcggct tgcaggtcgt cgtctcgtcg
     1501 gcccacacgt gttgcacacg gtcgtcctga gcgaaaccag cgcggtgcat ctcggcaagt
     1561 gcctgctcga acgtcatgcc gatcaggttc ggcatcaagt accactcggt gttcttgccg
     1621 atgttgccgt gccgcggatc gtcggtctgc acgcgcagcg acacgacaag ccgcgcgccc
     1681 tgcacgcgac ctgccgcggg aaactgatag cagacctcgc cgatctcgat gatcttgccg
     1741 tcgacgaagc tgccacacat gctgccgtcg tcgttgatat cgccttgcac gcccgcgcgt
     1801 cgcaacgcgg cgatcgcctc ctgcttgcgc agcttgaaca cgttgggaat cgtgaccgtg
     1861 ccgctcgagt cagcgccaga cgcacgcgac gacgacgacg acgacgtgaa gctcgacgtc
     1921 gccgcgcaac cagttagaaa tagaacgacg gatgcaaatg ccttcacagc tcacctcgac
     1981 aagttggttc accggcgctg acggctaggc gttcacgcgc ggcgcggtga tcggcgcgac
     2041 cagatccgca aatgctcgcc ggcgcgcctc ggtttcggga gcgagcgcat cggaaacgcg
     2101 ctcggacagt tgaacgtaga gcagctcgcc gacgtcgcga acgacgggcg gcttcacgac
     2161 agacacgatc ttgtcgcggc cgccgaccgc ggcgacgtgc gcgggtttga ggtacgtgcc
     2221 ccagcgcggc acgcgaatgt agtcctcgcc gagcttgcga cgatggattc cgtacgacga
     2281 gatctcaccg gggtcgggat gcagcgggcg tccattcagc gtgcggctca cgagcgacgt
     2341 ctcgatggcg acgatgtcag cgttcgtcgt gacgatgacg acgccattga gcgcaccaac
     2401 gagctcgacg atctcgtgct ggagatcgag ccataccttg gttgcttcat ccgatgcatc
     2461 ggcgggcacg tgccaggtcg agcaggcctc gaacggcaac gcaacgtcca ttgtcagcgg
     2521 acgcccgagg tcgatcccga agcgagccgt gtctagcgtg tcttgcttcg aacggctcac
     2581 accgaacagc acatgctgcg acgacgtcga cgtgatcgct tccgtgacgg actgccgtag
     2641 cttcgcggcc ggtacggtcc ccttgaacac gggcaccgcc gggcgctgcg gcgtgtgcca
     2701 gacattgatc aacttgaagc ggtagagcca gcgtggatcg acgacgagcg tcgcaagccg
     2761 tgccaaccgt tcctgatcgc catcgagcgc gcgatgaaac acgcagtgca agccgaccag
     2821 taaggtcatt ccccggtctc cggagcatcc gccggcggct cggcaacgtc gtcaccaaag
     2881 ccttttgcct ccgccgcgtc cgggcagaag tcgtagatat ccaggtccgg ggggtcgacg
     2941 accagctcct tgccgtcacg ttcgcagttg cggacctgtg ggtacttctc gaacaagctc
     3001 gccttgtctc gggcgtacca gttcttgagc ccctccgtat acgccttcat ctggcggcca
     3061 ccctcataca tggcgtcttg ggtccgtggc ttgatctcga tcaccttgca cgagctgccg
     3121 gtaagcacgc agtcggcgcg acaatcagag ccttcacgaa cctcgtttct gcagtcggta
     3181 ctcgtgattt tgaactccgc atacccactg cttccgctgc acatgctgct ctgccggtcc
     3241 gcgtgcacct tcttgccgta ctcgagcttc gcgcgaatct tcgggttgtt ggcgccctgt
     3301 agctcgtagc tcctgagctt ctcgaagttc cgccgattcg cttggattcc ttcgaccacg
     3361 cgcttggagc ctttgtagtg cgcgatcttg ctgtcggtcg cgcgcgattc gagccgatcc
     3421 gactgaccaa gcatcgtgcc gtacgcactg ctgatctgac tcgcccaccg atcggccacc
     3481 tctttcacct tcgcttccat ctcatactcg cccgcgcgac acatcacctc gcgcagatcg
     3541 tcacgatcct tctcggtgaa cgcgcggagc tgacgggcct cacgtagcca ggcgttgtag
     3601 tccttcgtga gctgcgtgac cgtgcctttt gtggtggcgg tgtagttgcg cagattctcc
     3661 agccccttgt cgacgtcggg atgcttctcg ccgagtgcga gccggggaca cgcgccttcc
     3721 gtcaccgcgc ggtagcgatc cttccagaag ctcgccatcc gcgacgcgtc atcgttgaag
     3781 gacgacgaca ccctggacca ctcgtccctg tcctccgtga atcgggcgta gtagaggacg
     3841 ctcgagaaat cgctgtcgtt ctgtttgagt gtggcgaggc gctcggtcca ggtctttcca
     3901 tactccttcg ccttatccgg cagcgttttg aacgcttcgt ccgcatcgtc gggacgaccg
     3961 acgtagtaac gaacgactgt ctggagatcc gcctcgtcgc tattgcactt gtctgcgaca
     4021 ccgtcggcga ggaactgcag gtctttcatc ctgctcaaga acttcgccgc cgcatcgaac
     4081 ccgtcgagat atctcgggta cttgtcgacg atgtcgttcg ctcgcgagtc actgcccttc
     4141 acccggtcga gcctgcccac caggtcacgc acctcacgtg cgtagctgag cgcatcattg
     4201 agatcaccgt tatcgcgatc ggagtcgaac cccgatagct cgctcgccat gctcttgatg
     4261 cggtcgtcga tattcctgat cagatccgcg cgatcagaat cgtcatccgc gtgcgcccgt
     4321 tgatccccga cgagcaatcc accaaccaca acacccgcga tcaacaccac aaccttgcta
     4381 ctcatccacg gacgctaaca cgcgggtctg acatttcgcc gcaacaaatc gttgcagcag
     4441 aatgcgactt caataaccag gggcaaccaa tggttgcgac gaaccggtct cagacctcga
     4501 cgagcatgcg gtcgggagcc tcgaggcgat ccttcaccgc gacaagaaac gacacggctt
     4561 cacggccgtc gacgacgcgg tggtcgtacg acagcgcgac gtacatcatc ggacgcacct
     4621 tcacttcatc gccgaccacc atcggacgct tcacgatgtt gtgcatgccg agaatgccgg
     4681 tctgcggata gttgagcagc ggcgtcgaca tcatcgaacc gtagatgccc ccgttcgtga
     4741 tcgagaacgt gccgccttgc agatcgtcga ggccgagctt gccgttacgc gccttctcgg
     4801 cgagcgcgag gatgcccttc tcgacgccgg cgaacgacag cgcgtcgcaa tcgcgcagca
     4861 ccggcacgac gagccccttt ggcgtcgaga ccgcgatgcc gaagtcgtag tgcttcttgt
     4921 agacgatgtt gccgccgact acctcggcgt tgatgccggg gaacagcttc gccgcagcga
     4981 cgcacgcttt gacgaagaac gacatgaagc cgagcttcgc gccgtgcgcc ttctcgaagc
     5041 tgtccttgta cttcgcgcgc agctccatga tcgcggtcat gtcgacttcg ttgaacgtcg
     5101 tcagcgacgc cgactcgtgc tgcgcttgca cgagccgctc ggcgacgcgc ttgcgcagcg
     5161 gtgacatcgg cacgatctcg tcgcgcggat cgacttgtgc gaggcggtct tcgaccgacg
     5221 gaccactcgg tgcagcgggt gcggcagcgc cgcgtgcagg cagcgcaccg gtctcgcgtg
     5281 cggtcgcgcg ttgcgacggc gtgagcttga gcagctgatc tttgtcgaac gattcaccgt
     5341 tgcccgactt cgcagcgggc ttcgcttgcg cggcagcttg cggcggcggt ggcggttgct
     5401 tcacgggcgc gggctgtgcg gcgacttgcg gctgtgcggc ggcagcagga gctggcttcg
     5461 ccggctcgac cttcgcagca gccggtttcg cagccgcctt gccggccttc cccgcatcga
     5521 ccgcaccgat cacgtcgccg actttgaccg tcgcaccgac cgcgaaccgc tgctcggtga
     5581 gcgcgcctgc gaccggtgac ggcagctcca ccgtgatctt gtccgtctcg agctctgcca
     5641 ccggctctcc atccgccacc gcgtcaccga cttgcttcag ccacttggca accaccgcct
     5701 cggagatcga ctcaccgagc tgtggaacta cgagatcagc catgggcgga ttttacctcc
     5761 gccgaggcta ccgggggtcg aatgcggcct tgagaatctg ctgctgttcg aggacgtgcg
     5821 ccttgtgtga accggttgcc gggctcgcgc tctcgacgcg cgaaatgaat tcatatttca
     5881 ggttgccgcc gcggtcgtgg aggatcgatt cgatgcgtgg agtgacgaat cggccggcgc
     5941 ccatgttgtg cggctcgtcc tgcacccaga agcactcccg cagcttcgtg tacggcgcga
     6001 tcgcgtcggc gacgaggtgc ggccaccacg gatacagctg ttcgagtctc acgatcgcga
     6061 cggtgtcgtc cttgcgcttg ctgcgctcgt cgacgagctc gtaatagatc ttgcccgtac
     6121 agaacaatac gcgcgtgacg tgcgccgggt cggcggtcgt gtcgtgcatg acgcgatgga
     6181 agcggccggt cgcgagctcg tcgagggtcg acgtcgccgc cggcagacgg agcaggctct
     6241 tcggcgtcat cacgacgagc ggcttgcgca ggcggcgaag cacctgcatg cgcaacaggt
     6301 ggaagacttg cgcgggcgtc gtcggctgcg cgacgcgcat gttgtgctcg gcgcacgact
     6361 ggaggtaacg ctcgaggcgc gcactcgagt gctccggccc ctgcccttcg tagccgtgcg
     6421 gcaacagcag cacgagaccg gagagtcgct tccacttgtc ttcggatgac gagatgaact
     6481 gatcgatgat gacctgcgcg ccgttcgcga agtcaccgaa ctgcgcttcc cacatcacga
     6541 gcgcgtcggg atagtcgagc gagtaaccga actcgaagcc catcacgccc gcttcggaca
     6601 gcggcgagtc gtagatgcgg cactggccct gatcgggatg gagctggccg agcagcatgt
     6661 gctcgcggcc gttgacgatg tcggtgatga ccgcgtggcg atggctgaac gtgccgcgcg
     6721 agacatcctg gccggtgagc cgcacgttga ccgcttgata gagcagcgaa ccgtacgtga
     6781 gcaattcgcc catgccccag tcgagcggct tgtcaccgcg gcccatctgc gcgcgctgtt
     6841 cgagcaagcg cacgatcttc gggtgcggcg tgaagccctg cggcacctcg gtcatgctct
     6901 gcgcgatctg ctcgagcagc ggacgcatca cgcgggtgtc gacttcttcg ggctcgtcgg
     6961 tgatgccgcc gcgatagccg cgccacatcg cggtcatcga tgcaccctcg gggcgctgct
     7021 tcgcggcctt tgctgcttcg agctcggtct cgagctcggc aacgcggcgc tgggtcatcg
     7081 cggtcgcttc atcggcggtg atcgtgtggt cggcgacgag cttgtgcgtg tagacctcga
     7141 cgggagactg cttctccttg atgcggtcgt acatgatcgg ctgcgtgaat gccggctcgt
     7201 cgttctcgtt atggccgtac ttgcgatagc agtacatgtc gatcacgacg tcggagccga
     7261 actgcgcgcg gtactcgcac gcgatttcga ccacgcgcgc gagcgcatcg aggtcctcgc
     7321 cgttgacgtg ccagatcggg cactcgatca tcttcgcgac gtcggtgcag tacggcgtcg
     7381 agcgctgctc agccggcgtc gcggtgaagc cgacctggtt gttgacgatg atgtggacgg
     7441 tgccgccggt gcgatagccc ggcaggttcg atagctgcag cgtctcgggc acgaggccct
     7501 gacccgcgaa agcagcatca ccgtggacga gcacgccgac gacacgcttg tgctcgacgt
     7561 cgccgtggcg ggtctgcttc gcacgcacgc ggccgacaac gaccggatcg acggcctcga
     7621 ggtggctcgg gttgaacgcg agcg
//
LOCUS       DASUFX010000062         7973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1816816, whole genome shotgun sequence.
ACCESSION   DASUFX010000062 DASUFX010000000
VERSION     DASUFX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816816"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFV99_02270"
     CDS             <1..282
                     /locus_tag="VFV99_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058151.1"
                     /translation="EPAELDREPAGGERADASSERKRLGDAIARGISALPDAQRAVLV
                     LREYYDLDYAEIADVLELDVGTVKSRLSRARTALREHLMATVPELGGRR"
     gene            279..2822
                     /locus_tag="VFV99_02275"
     CDS             279..2822
                     /locus_tag="VFV99_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5058152.1"
                     /translation="MSNDDELAEYDLSAWDAPPPPADLADAVIDRMGGTDVGIAVPVD
                     DQPASPRRAWIIAGAAVAALLLAVGVWSLIRSTHKAAPSSGSVLADKARTLDLDTVHA
                     DLDPGADVRWRRDGEVWLVEQRAGRAAWRVDGDEKLVIVAGAGLASVEATGANLRVEV
                     QMNAMDARVIGASALTAAAVAMVTVVVYEGHVNVGRGVGQQTVVVAPGTTYSVKNEDV
                     SQNLVGASRVAAEGGKKIAILGLQLDGKYGPDAPVVTQVMGATLRAAAQNDGRFQLSL
                     NADRELVDEKLLMNCADEATSCMSAIGADLGVDLLGFGHIEQLSSGYRITAKLLDVGK
                     QEIVSSGQWALGITEAQGKGLDTWGSHIYAELVGGSQTVCDAEALKEEGMQNINMGQH
                     AEALAKFEASLACRRDPYVTQLAFMESCSSSNSPKAKQYYKQLSPAQQAKFAQICIRQ
                     KVAYQDDFDFTGCDEVSCVLNNYDGACCQKFKKRSMPDGLDNAAIANGMAKIEDKALA
                     CGKPENAGAKVTMRVTVQPNGSVAGVLVEDESAPAGVTRPAPEVAQCIAAAINDAKFV
                     ATKNGGTFSYPFVFASPKTVAPTPPAHCDADALKEQGMEAVNRGQHATALAKFEASLK
                     CRNDPYVTQLVFMEACASKSSAKAKAYYVKLTSAQQAKFAQLCIRTNTDYQGGSAAPA
                     AAPAKCDADALKEQGMENINMGQHAAALAKFEAALACKGGDAQTIQLAFMESCNSANV
                     VKAKKYFKQMSQGAREKFEQICIRNKSAVRDYSDDPTAGFLQLVTKPEGAKVLIDGND
                     TGLRTPITGQTLPLKAGKHKVTIDVDGDRFAYPFTIQAGQTTKMSKDLQ"
     gene            2872..5394
                     /locus_tag="VFV99_02280"
     CDS             2872..5394
                     /locus_tag="VFV99_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058153.1"
                     /translation="MRMRAFAFLAVCALAACGDDSPPPTGVTTDLCAYEPVPATAHAG
                     GTVTAAQVRAGAAEKILDVPVGTALGGYTARAGFLGSAGVVDARKVKISGSFNPSIGV
                     TTAPRVKALALTAGDETVVILKMDAIFVYEGMVYDIESRLGPEYAGKVIVTASHSHSA
                     WAQFTGHGPLKLGAGQMRQLVYDRFLDTFEAAAREALDARRDAKIGFFYDPGFDPMNQ
                     INHDRRGDNDMLPGGNRKDNQFYMIRVDGTDNVPIAALPIFGEHGVLNDDDNPLASAD
                     APGALERVFQEQFDSKVVVMHVQSAGGDNSPNGHGGLDCNNKPGASGDPCFLWAAEEG
                     HGRGAASTMMAAWTAAGADMKSSIELEMLTRSVETGPKPETFTIRDGALKYAPFDNNR
                     VPDGMIYDGTGAIISPIDEYNAPVGAALCESETAMFPAAAILGDEGKLPYGSCLRLDV
                     AGEILGEIFSIDFGVDETHPVCETTRTTISALRIGDYVIGTMPGELTVMLKAYLTEHS
                     PVAADHTILFGYSQGHVGYMLTPEDWLKGGYEPSITFWGPLEAEHIAEQLVKLMPLAM
                     TPTREDGAKEGTTKVATAHMTDDFEIDNPAPDKGTVPATVPAGIWARTGQPTQAQPSA
                     QIPRVAGIATFVWIGDDPSTKTPHVTLQYETSTNVWTTVTRRSGRIVEDSEIVLAYTP
                     NPLQRSGPQTHYWVAEWQAVPWLGAPTVDSLNDRGGVPLGRYRFHVEGSGWTLDSDPW
                     QVVAGGESLLAMRTGGNIQTTVRWHAPKGWRLLDMALMSNQPVPVRSQSVTLELLDAA
                     DAVLSSANATTDAQGVVSVPNNASATKVRVTDRFGNVATQSL"
     gene            5520..6104
                     /locus_tag="VFV99_02285"
     CDS             5520..6104
                     /locus_tag="VFV99_02285"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX5058154.1"
                     /translation="MVPAVRSFVIFTAVVIAAGWVGIRYLPETAQARAEVVRPAPPRE
                     VQSISFDGRGLSVSTLREALSTQTGEVVDTAKLAHDREAMTEALAARGFLAAQVSPAQ
                     VVYTDRGAYVVFAVTQGPLFRVRSVTVTGASQLDAGVVTLGAGEIALADRITRAQEAL
                     ADRLAARGKRSDVRVNVSMDLAQSVVDIELVASR"
     gene            6150..>7973
                     /locus_tag="VFV99_02290"
     CDS             6150..>7973
                     /locus_tag="VFV99_02290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058155.1"
                     /translation="MGTVDQTMPALFASSGNGGAEIVPIAQLGAGPDGTAVLARKGEQ
                     LIELMQLSFGPDSPRWAPLEARLRTAGAVNHPGIRGVLGIESAPPTVMLEGDNSPPLA
                     ELVEQGADLQRVMRLLAEVARAIAAAHHVGLVHGRLDPWAVHVGASDRPRIELTGLAM
                     RSLDHAWVQRCIAPEARDGVAEAASDVYAIGALLDIFATGRKPGEAGASSDGVMAIIA
                     EATAADPDARPSATELVRKLAGAGTVVQRNTLRSAEIETARPSRPGLGLQLGRFELVK
                     QLGAGAMGEVWEARDNAGGPNVAIKLLKPEVAADADLLSRFRKEGRVLAKVGSPFIAN
                     FIDLNEDKGLHYLVLELVAGGSVAGALRRLGKLPERLALGIIADTCRALIEPHRLGIV
                     HRDIKPDNMMFVRAGIELEASPTGQLVKLGDFGIARLSEHNSGPEGATREGAVLGTPE
                     FMAPEQCQGAQITPATDVYALGCCLFALVAGRPPFVIQDDNQMAVILAHLKEPPPRLD
                     QLVPETAPAVAELVEKCLAKDPNARPQDAAEALAAIEQLLTGATALITAHPSPPIVRP
                     NLVQTYSFEWELQSSPEALWPFVSNTEKMNRAVGLPPVQFEI"
BASE COUNT         1410 a         2559 c         2791 g         1213 t
ORIGIN      
        1 gaacccgccg aactcgatcg cgagcccgcc ggtggcgagc gcgccgatgc gagcagcgag
       61 cgcaagcggc tcggcgatgc gatcgcacgc ggcatctcgg cactgcccga tgcgcagcgc
      121 gccgtgctgg tgcttcgcga gtattacgac ctcgattacg cagagatcgc cgacgtcctc
      181 gagctcgacg tcggcaccgt caagtcgcga ctcagtcgcg cgcgcacggc gttgcgcgag
      241 cacttgatgg caaccgtgcc cgagctcgga ggccgccgat gagtaacgac gacgagcttg
      301 ctgaatacga tctgtcggcg tgggatgcgc cgccaccgcc tgccgatctg gccgacgccg
      361 tgatcgatcg catgggcggc accgacgtcg gcatcgcggt tcccgtcgac gatcagccgg
      421 cgtcgccgag gcgcgcatgg atcatcgccg gtgcggcggt cgcggcgctg ctactcgcgg
      481 tcggcgtgtg gtcgctgatc cgttcgacgc acaaggcggc gccgtcgagc ggcagcgtgc
      541 tcgcggacaa ggcgcgcacg ctcgacctcg ataccgtgca cgcggatctc gatccgggcg
      601 ccgacgtacg ctggcggcgc gacggcgagg tgtggctcgt cgagcagcgt gcgggacgcg
      661 cggcatggcg cgtcgatggc gacgagaagc tggtgatcgt agccggagcc gggttggctt
      721 cggtcgaagc aacaggcgca aacctacgag tggaggtcca gatgaacgcg atggatgcgc
      781 gagtgatcgg cgcgagtgcg ttgacggctg cggccgtcgc gatggtgacc gtcgtcgtct
      841 acgagggtca tgtcaacgtc gggcgcggcg tcggccagca gacggtcgtc gtcgcgccgg
      901 gcacgacgta ctcggtgaag aacgaggacg tgagccagaa cctcgtcggc gcgagccggg
      961 tcgcggcaga gggcggcaag aagatcgcga ttctcggtct ccagctcgat ggcaagtacg
     1021 ggccggatgc accggtcgtc acgcaagtga tgggtgcgac gctgcgcgcg gcggcgcaga
     1081 acgacggtcg ctttcagctc tcgctcaacg ccgacaggga gctcgtcgac gagaagctgc
     1141 tcatgaattg cgcggacgag gcgacgtcgt gcatgtcggc gatcggcgct gacctcggtg
     1201 tcgacttgct cgggtttggc cacatcgagc agctgagctc ggggtaccgc atcacggcga
     1261 agctactcga cgtcggtaag caggagatcg tgagcagcgg acagtgggcg cttggcatca
     1321 cggaggcgca aggcaagggc ctcgacacgt ggggcagcca catctacgcc gagctcgtcg
     1381 gcggcagtca gaccgtgtgc gatgccgagg cgctcaaaga agagggcatg cagaacatca
     1441 acatgggcca gcacgcggag gcgctcgcga agttcgaagc atcgctcgcg tgcaggcggg
     1501 acccgtacgt cacgcagctc gcgttcatgg agtcgtgcag ctcgagcaac tcgccgaagg
     1561 cgaagcagta ctacaagcag ctctcgccgg cgcagcaggc caaattcgcc cagatctgca
     1621 ttcgccagaa ggtcgcgtac caggacgact tcgacttcac cgggtgcgac gaggtgtcgt
     1681 gcgtgctgaa caactacgac ggcgcgtgct gccagaagtt caagaagcgg agcatgcccg
     1741 acggtctcga taacgccgcg atcgcgaacg gtatggcgaa gatcgaggac aaggcgctcg
     1801 cgtgtggcaa gccggagaat gccggcgcca aggtgaccat gcgggtgacg gtacagccca
     1861 acggttctgt cgcgggcgtc ttggtcgagg acgaaagtgc gcccgctggc gtcacgaggc
     1921 ccgcgcccga ggtcgcgcag tgcatcgccg ccgcgatcaa tgatgcgaag ttcgtcgcga
     1981 cgaagaacgg cggcacgttc agctatccgt tcgtgttcgc gtcgccgaag acagtagcgc
     2041 cgacgcctcc cgcgcattgc gatgccgacg ccctcaagga gcaaggcatg gaggcggtca
     2101 acagggggca gcacgcgacc gcgctcgcca agttcgaggc gtcgcttaag tgcaggaacg
     2161 acccgtacgt cacgcagctc gtgttcatgg aagcgtgcgc cagcaagagc tcggcgaagg
     2221 ccaaggccta ctacgtgaag ctcacctcag cgcagcaggc gaagttcgcc cagctctgta
     2281 tcaggacaaa caccgactat cagggtggca gtgcggcgcc ggcagctgcc ccggcaaagt
     2341 gcgacgccga tgcacttaaa gagcaaggca tggagaacat caacatgggc cagcacgcgg
     2401 cggcgctcgc gaagttcgag gcagcgcttg cgtgcaaggg tggggacgcg caaaccattc
     2461 agctggcgtt catggagtcg tgcaacagcg ccaacgtggt gaaggcgaag aagtacttca
     2521 agcagatgtc acagggcgcg cgcgagaagt tcgagcagat ctgcatccgt aacaagagtg
     2581 cggtccgtga ctacagcgac gatccgaccg cggggttcct ccagctggtc acgaagcccg
     2641 aaggcgcgaa agtcctgatc gacggcaacg acactgggct gaggacgccc atcactggtc
     2701 agacccttcc actcaaagcc ggcaagcaca aggtcacgat cgacgtcgac ggcgatcggt
     2761 tcgcatatcc attcacgatt caggcgggcc agaccacgaa gatgtcgaag gacctccagt
     2821 agcgaaaacg atcacacgag gcgccccggg ctcgtgatat gccctccctc gatgaggatg
     2881 agggccttcg cgtttctagc ggtctgcgcg ctcgccgcgt gcggagacga ttcaccaccg
     2941 ccgaccggag tcacgaccga cctctgcgcg tacgagccag tgccggcgac tgcacatgca
     3001 ggcggcacgg tgacggcagc gcaggtccgc gcaggcgcag cggagaaaat tctcgacgtg
     3061 ccggtcggca ccgcgctcgg tggctacacg gcccgcgccg gtttcctcgg ctccgccggc
     3121 gtcgtcgacg cgcgcaaggt gaagatctcc ggctcgttca atccgtcgat cggcgtgacg
     3181 accgcgccgc gcgtgaaagc gctcgcgctc accgcgggtg acgagacagt ggtcatcctc
     3241 aagatggacg cgatcttcgt ttacgaaggc atggtctacg acatcgagtc gcggctcggt
     3301 cccgagtacg cgggcaaggt gatcgtcacc gcgtcgcata gccactcggc gtgggcgcag
     3361 ttcacgggcc acggtccgct caagctcggc gccggtcaga tgcgccagct cgtctacgat
     3421 cggttcctcg acacgttcga ggcggcggca cgcgaagcgc tcgatgcgcg gcgcgacgcg
     3481 aagatcgggt tcttctacga ccccggcttc gatccgatga atcagatcaa ccacgatcgc
     3541 cgcggcgaca acgacatgct gccgggcggc aatcgcaagg acaaccagtt ctacatgatc
     3601 cgtgtcgacg gcaccgacaa cgtgccgatc gcagcgctgc cgatcttcgg cgagcacggc
     3661 gtgctcaacg acgacgacaa cccgctcgcg tcggccgacg caccgggcgc gctcgagcgc
     3721 gtgttccagg agcagttcga ttcgaaggtc gtcgtcatgc acgtgcagag cgcgggcggg
     3781 gacaactcgc cgaacggtca cggtggcctc gactgcaaca acaagccggg cgcgtcgggt
     3841 gatccgtgct tcttgtgggc ggcggaagaa ggtcacggtc gcggtgcggc gtcgacgatg
     3901 atggcggcgt ggaccgcggc gggcgcggac atgaagagct cgatcgagct cgagatgctc
     3961 acgcgctcgg tcgagaccgg accgaagccg gagacgttca cgattcgcga tggcgcgctg
     4021 aagtacgcgc cgttcgataa caaccgcgtg cccgacggga tgatctacga cggtaccggc
     4081 gcgatcatct cgccgatcga cgagtacaac gcgccggtcg gcgcagcgct gtgcgagtcg
     4141 gagactgcga tgttcccggc cgccgcgatc ctcggcgacg agggcaagct cccctacggc
     4201 tcgtgcctgc gcctcgacgt cgcgggcgag atcctcggcg agatcttctc gatcgacttc
     4261 ggtgtcgacg agacgcatcc ggtttgcgag acgacgcgca cgacgatctc ggcgctgcgc
     4321 atcggcgact acgtgatcgg cacgatgccc ggtgagctca cggtgatgct caaggcgtat
     4381 ctgaccgagc actcgccggt cgccgctgat cacacgatcc tgttcggtta ctcgcagggc
     4441 cacgtcggtt acatgctgac gcccgaggat tggctcaagg gcggctacga gccgagcatc
     4501 acgttctggg ggccgctcga agccgagcac atcgccgagc agctcgtgaa gctgatgccg
     4561 ctcgcgatga caccgacgcg cgaagacggc gcgaaggaag gcacgacgaa ggtcgcgacc
     4621 gcgcacatga ccgatgactt cgagatcgac aatccggcgc cggataaagg cacggtgccg
     4681 gcgacggtgc cggcgggcat ctgggcgcgc acgggccaac cgacgcaggc gcagccgagc
     4741 gcgcagattc cccgcgtcgc cggcatcgcg acgttcgtgt ggatcggcga cgacccgagc
     4801 acgaagacgc cgcacgtcac gctgcagtac gagaccagca cgaacgtgtg gacgacggtc
     4861 acgcggcgca gcggccgcat cgtcgaggac agcgagatcg tgctcgcgta cacgccgaac
     4921 ccgttgcaac gcagcggacc gcagacgcac tactgggtcg cggagtggca ggcggtgccg
     4981 tggctcggcg caccgaccgt cgacagcttg aacgatcgcg gcggcgtgcc gctcggccgc
     5041 tatcgcttcc acgtcgaagg cagtggctgg acgctcgaca gcgacccgtg gcaagtcgtc
     5101 gcgggtggtg agtcgctgct cgcaatgcgc accggcggca acatccagac gaccgttcgc
     5161 tggcacgcac cgaagggttg gcgcctgctc gacatggcgc tgatgtcgaa ccaaccggtg
     5221 ccggtccgga gtcagtcggt gacgctcgag ctgctcgacg cggcggacgc ggtgctgtcg
     5281 agcgcgaacg cgacgaccga cgcgcagggc gtggtcagcg tcccgaataa tgcgtcggcg
     5341 acgaaggtgc gcgtgaccga ccggttcggc aacgtggcga cccagtcgct ctaaggaccg
     5401 acacgtacgt tgtggggacc cggggcgccg gggatacggg actgtgatgg gcagttcgcg
     5461 gcggatccca cgtaggtagg cctggttctg ccaggttggg cgtggtcttg cccttgcaca
     5521 tggtgccggc cgtgcggtcg ttcgtaatct tcacggcggt ggtcatcgcg gcaggctggg
     5581 tcggtatccg atacctgccc gagaccgcgc aggcgcgggc cgaagtcgtg cgcccggcgc
     5641 cgccgcgtga ggttcagagc atctcgttcg acggccgcgg cctgtcggtc tcgacgctgc
     5701 gcgaggcgct gtcgacgcag accggcgaag tggtcgatac cgcaaaactc gcgcatgacc
     5761 gcgaggcaat gaccgaggcg ctggccgcgc gtggctttct cgccgcgcag gtgtcgccgg
     5821 cgcaggttgt ctacaccgac cgcggcgcct acgtggtgtt tgcggtgacg cagggaccgc
     5881 tgttccgtgt gcgttcggtg acagtgaccg gcgcgtcgca gctcgatgcg ggcgtcgtca
     5941 cgctcggtgc cggcgaaatc gcgctggccg accgcatcac gcgggcgcaa gaagctctcg
     6001 cagaccggct cgcggcacgt ggcaagcgca gcgatgtgcg tgtcaacgtg tcgatggacc
     6061 tggcccaaag cgtcgtcgat atcgagctgg tcgccagtcg ctagggcagg aaatccgccg
     6121 gcgccggttt tgacggtatc ctgtgggccg tgggaacggt cgaccagacc atgcctgcgc
     6181 tgtttgcctc gtcgggcaat ggcggcgcag agatcgttcc gatcgcgcag ctcggcgcgg
     6241 gtcctgatgg caccgcggtg ctcgcgcgca aaggcgagca gctgatcgag ctgatgcagc
     6301 tgtcgttcgg gccggactcg ccgcgctggg cgccgctcga agcgcgactg cgcactgcag
     6361 gtgcggtgaa tcatcctggc attcgcggtg tgctcggcat cgagagcgcg ccacccactg
     6421 tgatgctcga aggcgataac tcgccgcctc tcgcagagct ggtggagcag ggcgccgatc
     6481 tgcagcgcgt gatgcggcta ctcgccgagg tcgcgcgcgc gatcgcggcg gcgcatcacg
     6541 tcggcctcgt gcacggacgc ctcgatccgt gggcggtgca cgtcggcgcg agcgatcggc
     6601 cgcgcatcga gctcaccggt ctcgcgatgc gatcgctcga tcacgcatgg gtccagcgct
     6661 gcatcgcacc cgaagcgcgc gacggcgtgg cggaagcggc atcggacgtc tatgcgatcg
     6721 gtgcgctgct cgacatcttc gctactggtc gcaaacccgg agaagccggc gcgtcgagcg
     6781 acggcgtgat ggcgatcatc gcggaggcaa ccgccgcgga tcccgacgcg cgaccgagcg
     6841 cgaccgagct cgtgcgcaag ctcgctggtg cgggcacggt cgttcagcgc aacacgctgc
     6901 gcagcgccga gatcgaaacc gcgcggccgt cgcgcccggg gctcggtctg cagctcggcc
     6961 gcttcgagct cgtgaagcag ctcggcgccg gcgcgatggg cgaggtgtgg gaagcgcgcg
     7021 ataacgccgg cgggccgaac gtcgcgatca agctgctcaa gcccgaggtc gccgccgacg
     7081 cggatctgtt gagtcgcttc cgcaaggaag gccgcgtgct cgcgaaggtc ggcagcccgt
     7141 tcatcgcgaa cttcatcgat ctcaacgagg acaaagggct gcactatctc gtgctcgagc
     7201 tcgtcgccgg cggcagtgtc gccggtgcat tgcgccgact cggcaagctg cccgagcgac
     7261 tcgcgctcgg catcatcgcg gacacgtgtc gcgcgctcat cgagccgcat cgcctcggca
     7321 tcgttcatcg cgatatcaag cccgacaaca tgatgttcgt gcgcgcgggc atcgagctcg
     7381 aggcgtcgcc gaccggtcag cttgtcaagc tcggcgactt cggcatcgcg cgtctcagcg
     7441 agcacaacag tggtcccgag ggcgcgacgc gcgaaggcgc cgtgctcggc acgccggagt
     7501 tcatggcgcc cgagcagtgt cagggcgcac agattacgcc cgcgaccgat gtctacgcgc
     7561 tgggctgctg cttgttcgcg ctcgtcgcag gtcgcccgcc gttcgtcatc caagacgaca
     7621 accagatggc ggtaatcctc gcgcacctga aggagccgcc gccgcgcctc gatcagctcg
     7681 tgccagagac cgcgccggcg gtcgccgagc tcgtcgagaa atgtctcgcg aaggatccga
     7741 acgcgcgccc acaggacgcc gccgaggcac tcgctgcgat cgagcagctc ctcacgggcg
     7801 cgaccgcgct gatcaccgcg catccgtcgc cgccgatcgt gcgcccgaac ctcgtgcaga
     7861 cgtactcgtt cgaatgggag ctgcagtcgt cgcccgaggc gctgtggccg tttgtctcga
     7921 acaccgagaa gatgaatcgc gccgtcggct tgccgccggt gcagttcgag atc
//
LOCUS       DASUFX010000063         4611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_565988,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000063 DASUFX010000000
VERSION     DASUFX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565988"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFV99_02295"
     CDS             complement(<1..535)
                     /locus_tag="VFV99_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058156.1"
                     /translation="MGRTIPIAPHPLLDAGAFITRFAQPLGELTTKIDAGVTPPLSTS
                     DAVRTEIRQLLRHGGFKPAGRSKPASEYLVAAHTEGRFPTINAAVDACNVASLWSGLP
                     ISLVDLDRVVGDLSIGICSAGTNYVFNPSGQVIDASGLLALFDTEGPTGTPVKDAQRT
                     KTHDGTLSTISIIWGTNQ"
     gene            593..2692
                     /locus_tag="VFV99_02300"
     CDS             593..2692
                     /locus_tag="VFV99_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058157.1"
                     /translation="MSAAGEAATSRPIRLGRYELITRIGQGGMAEVQLALQRGPAGFE
                     KLVVVKLVHNNLASQKAFVDMLLDEARVAALVKHPNVVDIYDLGQADGRYFIAMEYLE
                     GEPLLAVLRAGREGRRLDTLSTGRLIADTAEGLDAAHELRSMSGDRLELVHHDISLGN
                     IVVLYNGQVKLVDFGVAKATQSAGTRTKVQGKFSYMAPEKLKGAAGDRRSDIFSLGCV
                     MWEALTLKRLFRGGNDAETMKQVLEMAIQPPSKINGEVPPEYDPIVMRALDRDPNRRH
                     GTAKEMAVEIEELLRKRGYGAKNDKIARYMQETFKPHIDARKKLVQEVVNKGSASAEV
                     LDAAFSEPILASGSPVEPVGGSDFSLSYKRVDAPPPKPKLPVPNRHVSPAEGVPIPTA
                     AWADSSPRTQISTPPVHPMFDGGNTGTEIAPLPDMSSVVDQPESKPRGKSIGDRVRAL
                     TERVRPLAGRVRPLLVRGRDQAKAILADKKKRLYVAAGAGVLLLLIIIIAIASGGDDK
                     KAVAADGSGSGSEQHIVTGPGSDVIENNGNGSGSGSAIPEVGSDDIVIEHGSNDVGSN
                     AGSAINTEKNVKTEKTEKTVKTEKTVRNEKTVRNEKNEKTEKTEKVVQPQIDVVETVN
                     EGLKAFVTGDTKQALTLLKQAKAAKPGYAPTYRVLGKVYKKLGDNASAKASFQRYLKL
                     APTAPDAEQIRKEMEEL"
     gene            2692..3567
                     /locus_tag="VFV99_02305"
     CDS             2692..3567
                     /locus_tag="VFV99_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058158.1"
                     /translation="MRTAIVALVTAIALFTTVVAGPRKVIVLPLDGNASVAQKTQLND
                     SVAKIAKEKIAGDVTRGDTTFAETAAAVGCDPANPACADTVRSTLAVDELVYGTAKTS
                     DGKTTVTVYWASKGSPTTSRVAIISETDTGDKAEPALTTAFDTSVPTGSDVIVGSGSE
                     LGSGSGSETPERIPGKSFFDSRDRKLGVGLAAGGVIAIVIGLSLWSSKSDLQDQIDNH
                     PTRTLVDLQDLHSLEDRASSKALWGNILVGLGLGLGATGAYFLYRDHESRNTTVTPAP
                     VEAGTGMTLVLGGRW"
     gene            3564..4463
                     /locus_tag="VFV99_02310"
     CDS             3564..4463
                     /locus_tag="VFV99_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058159.1"
                     /translation="MKPAAVGTLVGTVAGVLLWSCSVNRVSDGYRCDTNADCALDRVC
                     TSGYCVIGKNPLLDAPPRDAAVCPAICGGTCDFQTTTCTITGTGSGNLACPPGWNCII
                     NCGSTGACGTISCTQAQSCDIHCTADSACLGITCQTKDCTVECVGQKACGNIGCTSGD
                     CTADCSGGGGSAACGTISCTSGNCDATCTGSNACGGISCTTGRCRTDCSGGSAACGTL
                     NCGLGECSATCVGSGSGSDSSCGSVNCANSCKCDVSCDGTTNICPASMMCPDRAPGAA
                     YCTIDGSNGTRCFSSFAAQCNNC"
BASE COUNT          912 a         1488 c         1488 g          723 t
ORIGIN      
        1 gttggttggt gccccaaatg atcgagatcg tgctcagcgt gccgtcgtgt gtcttcgtgc
       61 gctgagcatc tttgaccggt gtccccgtgg ggccttcggt gtcgaacagt gccaagaggc
      121 cactggcatc gatgacttga ccggagggat tgaacacata gttcgtcccg gcggaacaaa
      181 tcccgatcga gagatcgcca accacacggt cgagatcgac cagcgagatc ggaagccccg
      241 accacagcga cgcgacattg cacgcatcga cggccgcatt gatcgtcggg aagcgccctt
      301 ctgtgtgcgc agctacgagg tactccgacg cgggcttgct ccggcccgct ggcttgaagc
      361 cgccatgtcg taagagctgg cgaatttcag tgcgaacggc atcgctcgtc gacagaggcg
      421 gagtcactcc cgcgtcgatc ttcgtcgtga gctcgcctag cggctgggca aatcgagtga
      481 tgaacgcgcc ggcgtcgagg agcgggtgtg gcgcgatcgg gatggtgcgg cccataccta
      541 cacattagct tcgacgcgcc ctcgcaaacc ccagctgcga tacactcttt gcgtgagtgc
      601 tgcgggggag gccgctacat cgcgccccat ccgcctcggg cgctacgagt tgatcactcg
      661 tatcggtcaa ggcgggatgg cagaagtgca actcgcactt cagcgcggtc cggcggggtt
      721 cgagaaactg gttgtcgtca aactcgttca caacaatctc gcgagccaga aagcgttcgt
      781 ggacatgttg ttggacgagg cgcgcgtcgc tgcactcgtc aagcatccga acgtggtcga
      841 catctacgac ctcggccaag ccgatggtcg ctacttcatc gcgatggagt acctcgaagg
      901 cgagccactg ctcgccgtct tgcgcgccgg tcgcgaaggc agacgactcg acacgctctc
      961 gacgggtcgc ttgatcgccg acacggcaga aggactcgat gccgcgcacg agctgcgcag
     1021 catgtcgggc gaccgactcg agctcgtgca ccacgacatc tcgctcggaa atatcgtcgt
     1081 tctctataac ggccaggtta agctggtgga tttcggtgtc gcgaaggcaa cgcagagcgc
     1141 gggcacgcga acgaaggtcc agggcaagtt cagttacatg gcgcccgaga agctcaaggg
     1201 cgcggccggc gatcgacgca gcgacatttt ttcgttgggc tgcgtgatgt gggaagcgct
     1261 cacgctcaag cgcctgttcc gcggcggcaa cgacgccgag acgatgaaac aggtactcga
     1321 gatggcgatc caaccgccgt cgaagatcaa cggcgaagta ccgcccgagt acgacccgat
     1381 cgtgatgcgc gcactcgatc gcgatccgaa tcgcaggcac ggcaccgcga aggaaatggc
     1441 ggtcgagatc gaggagcttc tgcgcaagcg cggctacggc gcgaagaacg acaagatcgc
     1501 gcgctacatg caggagacgt tcaagccgca catcgacgcg cgcaagaagc tcgtccagga
     1561 agtcgtcaac aagggctcgg cgagtgccga ggtgctcgac gccgcattca gcgagccgat
     1621 cctcgcgtcg ggctcgcccg tcgaaccggt cggcggcagc gacttctcgc tgagctacaa
     1681 gcgcgtcgac gcgccgcctc ccaaacctaa gctgcccgtt cccaaccggc acgtttcgcc
     1741 ggccgagggc gtgccgattc caaccgcggc atgggccgac tccagcccgc ggacgcaaat
     1801 cagcacgccg cccgtgcatc cgatgttcga cggcggcaac accgggactg aaatcgcgcc
     1861 gctgcccgat atgagcagcg tcgtcgatca gccggagagc aaaccgcgcg gcaagtcgat
     1921 cggcgatcgc gtccgcgcgc tgaccgaacg agtgcgcccg cttgccggac gcgtgcggcc
     1981 actgctcgtg cgcggccgcg atcaagcgaa ggcaatcctc gccgacaaga agaagcggct
     2041 ctacgtggcc gccggcgccg gcgtgctgtt gctgttgatc atcatcatcg cgatcgcgag
     2101 cggcggcgat gacaagaaag ccgtcgcagc cgatggcagc ggtagcggca gcgaacaaca
     2161 catcgtcact ggacccggta gcgatgtcat cgagaacaac ggcaacggca gcggcagcgg
     2221 cagcgccatc cccgaggtgg gtagcgacga catcgtgatc gagcacggca gcaacgacgt
     2281 cggcagcaac gccgggtcgg cgatcaacac cgagaagaac gtcaagaccg agaagaccga
     2341 gaagaccgtc aagaccgaga agaccgtcag gaacgagaag accgtcagga acgagaagaa
     2401 cgagaagacc gagaagacag aaaaggtcgt ccagccgcag atcgatgtgg tcgagaccgt
     2461 caacgaaggc ctcaaggcgt ttgtcaccgg cgataccaag caggcactga cgctgctgaa
     2521 gcaagcgaag gctgcgaagc ccggctacgc gccgacgtat cgcgtgctcg gcaaggtcta
     2581 caagaagctc ggcgacaacg cgtccgcgaa agcctcgttc cagcgctatc tcaagctcgc
     2641 ccccaccgcg cccgacgcag aacaaatccg caaggaaatg gaagagctct agtgcgaacc
     2701 gccatcgttg ctctcgtcac cgcgatcgcg cttttcacga cggtcgtagc cggcccgcgc
     2761 aaagtgatcg tgttgccgct cgatggcaat gcatcggtcg cgcagaagac ccaactgaac
     2821 gactcggtcg cgaagatcgc gaaggaaaag atcgccggcg acgtcacgcg cggtgacacc
     2881 acgtttgccg agaccgcagc cgccgtcggc tgtgaccccg cgaatccggc atgcgccgac
     2941 accgtgcgct cgacgctcgc cgtcgacgag ctcgtgtacg gcaccgcgaa gacgagcgat
     3001 ggcaagacca cggtcaccgt gtattgggcg agcaaaggat cgccgacgac gtcacgcgtg
     3061 gcgatcattt cggagaccga caccggcgac aaggccgagc ctgctctgac cacggcgttc
     3121 gataccagcg tgcccaccgg ctccgatgtc atcgtcggct cgggctccga gctcggctcc
     3181 gggtcgggct ctgaaacgcc cgagcggatt cccggcaaga gcttcttcga ctcgcgtgac
     3241 cgcaagctcg gcgtcgggct cgcggcaggc ggcgtgatcg cgatcgtgat cggcctgtcg
     3301 ctgtggtcgt cgaagagcga cctgcaggac cagatcgaca atcacccgac gcgcacgctc
     3361 gtggacctgc aggacctcca ctcgctcgag gatcgcgcca gcagcaaggc gctgtggggc
     3421 aacatcctcg tcggcctcgg actcggtctc ggcgcgaccg gcgcgtactt cctctatcgc
     3481 gaccacgaga gccgcaacac gaccgtgacg ccagcgcctg tcgaagcagg aaccggcatg
     3541 acgctcgtgc tcggagggcg ctggtgaagc cggccgccgt cggtacgctc gtcggtaccg
     3601 tcgctggggt gctgttgtgg agctgctcgg tgaatcgcgt ctccgacggc tacagatgcg
     3661 acacgaacgc ggattgcgca ctcgatcgcg tctgcacgag cggctactgc gtgatcggca
     3721 agaacccgct gctcgatgcg ccgccacgcg acgccgcggt gtgtcccgcg atctgcggcg
     3781 gcacgtgcga cttccagacg acgacgtgca cgatcaccgg cactggcagt ggcaacctcg
     3841 cgtgcccgcc gggctggaac tgcatcatca actgcggctc gaccggtgcg tgcggaacga
     3901 tcagctgcac gcaggcacaa tcgtgcgaca tccactgcac cgccgacagc gcctgcctcg
     3961 gcatcacctg ccagaccaag gactgcaccg tcgagtgcgt cgggcagaag gcgtgcggca
     4021 acattggctg cacgagcggc gactgcacag cagactgctc gggcggtggc ggtagcgcgg
     4081 catgcggcac gatctcgtgc acgagcggca actgcgatgc gacgtgcacc ggcagcaacg
     4141 cgtgcggcgg catcagctgc acgaccggcc gctgccgaac cgactgctcc ggtggcagcg
     4201 ccgcctgcgg aacgctcaac tgtggcctcg gcgagtgttc tgcgacctgc gtcggctcgg
     4261 gatcgggctc cgattcgtcc tgtggctccg tcaactgcgc gaattcgtgc aaatgtgacg
     4321 tttcctgcga cggcaccacg aacatctgcc cggcttcgat gatgtgcccg gatcgcgccc
     4381 ctggcgcagc ctactgcacg atcgatggca gcaatggcac gcgttgtttc tcttcgttcg
     4441 ccgctcagtg caataattgt tgaccgatga atgcagtcag cttgggcgcg ctgatgagcg
     4501 tggcgcttat cgccgcgtcg tgtcttgtcg agcgcccatc gagcgcgttc gagtgcaaga
     4561 ccaccgctga ctgcgccagc ttcacggaca accgccagtg caccggcggc t
//
LOCUS       DASUFX010000064         7254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_427540,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000064 DASUFX010000000
VERSION     DASUFX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427540"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFV99_02315"
     CDS             <1..632
                     /locus_tag="VFV99_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5058160.1"
                     /translation="FSFADYYDPRHMGFRVLRVINEDYVAAGRGFGMHPHHDMEIVTY
                     VLEGELGHRDSMGTGSVIRPGDVQRMSAGTGVTHSEMNASKTQRVHLLQIWILPDKQG
                     YTPSYEQKAFSDADRRNKLRVVASPDGRDGSVTIHQDATISASLLSSGQRVEHTLDKN
                     RYGWLQVARGSVELDGEKLIAGDGAAIRGPGTVPIKAIDDAEFLLFDLP"
     gene            complement(629..1297)
                     /locus_tag="VFV99_02320"
     CDS             complement(629..1297)
                     /locus_tag="VFV99_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5058161.1"
                     /translation="MPGRKPVRRLLRAAAAVRPRRRIRLDNDERRAQLLQLARKAFSD
                     RSYDDVSIDDLAREAKISKGLLYHYFPTKRDLYVAGLREIAEELVQRVLTIPPDLAPI
                     ERVRAGIEAYLDHISQHSRAYVSLMRGGIGSDPEVAAVVEGVRKRLADNFLEQTPFAQ
                     MLSKDVRFQTAVRGWIGFVEGATIDWCANPRLSRDDLRELLTTILFSIITAVAPQLIK
                     DNIR"
     gene            1357..2163
                     /locus_tag="VFV99_02325"
     CDS             1357..2163
                     /locus_tag="VFV99_02325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5058162.1"
                     /translation="MAIPTPRKLAVDLSNVPRHWLAGNPAATAISNGINMLFPHGERF
                     FVRSVHHYLDEIKDPDLRAQVKAFFKQEGHHASAHDDFNEALRRQGFEIDRFLKRYQQ
                     ITNFVEARMPAKINLAGTAAAEHFTAILADGAFTKGVLDALHPSVQQLLAWHAAEEIE
                     HKAVAYDVLQQIDPSYAVRVAGLAYATVMLGMFWVWATTMLLRQEKLGWREILRQLGA
                     MRKTDPVIRRVFIAGIRQYIRRDFHPRQNANEHLAAQWFAARGFTLPEAA"
     gene            2160..2972
                     /locus_tag="VFV99_02330"
     CDS             2160..2972
                     /locus_tag="VFV99_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5058163.1"
                     /translation="MNLKDRVVVVTGAGSGIGRATALAFAAEGARVAACDVDEKRLES
                     LASELGDRKLLVRRVDVSDRLSMKLFADAVHELVPAADVIVNNAGVAVGGTFLDTTLD
                     DWDWLLGVNLRGVVHGCHYFIPKMVARASGGHVVNVSSILGIYPAPNVTAYVASKFAV
                     RGFSQSLRAELAPHKIGVTAICPGMINTAIVADGRMSGNVGSRKDKMVATFHKRGLAP
                     AKVAEAILDAVRSNPAVRPVGADAMVMAALMRVAPRTFNRLAGTIQRRFGVG"
     gene            3288..3965
                     /locus_tag="VFV99_02335"
     CDS             3288..3965
                     /locus_tag="VFV99_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX5058164.1"
                     /translation="MPKAAPKFVGFDRDALQFLHELTLEMNREWFEANKARYQARWVE
                     PMTALLGEVAGRLAKVYAPIKLGPPKLFRIYRDTRFSKDKSPYKTHVAGVIPLREGCT
                     AMYMHIGIDDEFVGVGTYYFEDSQLPRWRKLVAADKTGKEIAAIVTKLRKAGYKVGGH
                     EDYKKVPKGFDPEHPRAEFLKMRGLTGGFPAIPKGLLHKPALADWLFTHAKATAPMVS
                     WLYRNVK"
     gene            4091..5080
                     /locus_tag="VFV99_02340"
     CDS             4091..5080
                     /locus_tag="VFV99_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5058165.1"
                     /translation="MKLATLRDGSRDGRLLVVRRDGEAGAPAPDAWPTLQRALDDWDA
                     AEPALRALQDQLDRGALDGVTIDPNRLGAPLTRAYEWVDGSAFLNHVILVRKARGAEP
                     PKTLETDPLIYQGGSGDMLGPRDPIELHEPAWGLDFESEVCVVLGDTPIGTRAADAHH
                     HVRLLMLANDCTLRNLIPEELAKGFGFFQSKPATAFGPFAITPDELGDAWRGGKAHIR
                     VRSVYNEAQVGDCEAGPEMHFSFFDLIQFIAKTRRFTAGTILGSGTVSNADRSRGISC
                     LAERRMIETIEGGKPLTPFMSVGDHIEIEAIDPAGASPFGKIDQRVVAAGGSR"
     gene            5077..5721
                     /gene="maiA"
                     /locus_tag="VFV99_02345"
     CDS             5077..5721
                     /gene="maiA"
                     /locus_tag="VFV99_02345"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX5058166.1"
                     /translation="MKLVLHNYWRSSASQRVRIGLGLKQLAYDYVVVNILHKDQHADA
                     YRARNPMAQVPTLEITEDNGTVHALTQSLPILEYLDERFPEKPIMPRDPYLRARTRAL
                     AEIVNSGIQPLQNLTTTKAVKAFGGDEVAWPKDFIANGLAAFDRAASDIAGKFCVGDA
                     PTIADCCLIPQLTSARRFGVDISKFTLLLGIEERCLAMPAFTNAAPDRQPDAVK"
     gene            5729..6883
                     /locus_tag="VFV99_02350"
     CDS             5729..6883
                     /locus_tag="VFV99_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5058167.1"
                     /translation="MAKQESLGIKRLEGIHYYVHDLDRSRQFYCGKLDFAETWRSSPE
                     LEKTGRQKSACFSAGNINVVCSTPTGGGGGGRAARFLSKHPDGVGTLIFEVEDAEKTF
                     RLLEERGGTPIEDIQTFKDDSGGTLRQFSITTPFGDCTFRFRERRGFKALYPGAVAHD
                     GGGKNKFGFQEIDHVTSNFQTMKPMLLWLEHVLGFEQMWEVEFHTSDVDPNRKTGSGL
                     KSIVMWDPQSNTKFANNEPSRPFFKSSQINIFNEELRGDGVQHVAMTVADIVSAVRAL
                     RGNGVTFMPTPGTYYDMLPERITQSGIKQIDEDLKILRELEILVDGDHFHAYMLQIFL
                     KEASGLYGDPKAGPFFYEIIQRKGDRGFGAGNFRALFESIEREQRERGLI"
     gene            6883..>7254
                     /locus_tag="VFV99_02355"
     CDS             6883..>7254
                     /locus_tag="VFV99_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5058168.1"
                     /translation="MLDRMQVGEVAKKHHIQLRGTDGELRFEECFTRDGFDGPYTIMY
                     HLRRPHTQRIAPAAHGWAAPVAVTERPLAKRHYKTGDLAKQGSGAQVDARIPLVFNAD
                     VTCGVAFPTAEDPVYVVNGDAD"
BASE COUNT         1301 a         2386 c         2341 g         1226 t
ORIGIN      
        1 cgttttcgtt cgcggactac tacgacccac gccacatggg cttccgcgtg ctgcgcgtga
       61 tcaatgaaga ctacgtcgca gcagggcggg gcttcggcat gcacccgcat cacgacatgg
      121 agatcgtgac gtacgtgctc gaaggcgagc tcggccaccg ggacagcatg ggcacgggct
      181 cggtgatccg gcccggtgac gtgcagcgga tgagcgcggg gaccggcgtc acgcacagcg
      241 agatgaacgc gtcgaagacg cagcgcgtgc acctgctcca gatctggatc cttcccgaca
      301 aacagggcta cacgccgagc tacgagcaga aggcgttcag cgatgccgac cgtcgcaaca
      361 agctgcgcgt ggtcgcatcg cccgatggcc gcgacggctc ggtcacgatc caccaagacg
      421 ccacgatctc ggcgtcgctg ctgtcgtccg gccagcgcgt cgagcacacg ctcgacaaga
      481 atcgctacgg ctggctccag gtcgcgcgtg gctccgtcga gctcgacggc gagaagctca
      541 tcgctggtga tggcgctgcg atccgtgggc cgggcaccgt gccgatcaag gcgatcgacg
      601 acgccgagtt tctgctgttc gatctaccct aacgaatgtt gtccttgatc aactgcggtg
      661 cgactgcggt gatgatcgag aacaggatcg tggtgagaag ctcgcgcaag tcgtcgcgcg
      721 atagccgcgg gttcgcgcac caatcgatcg ttgcgccttc gacaaagccg atccagccgc
      781 gcacggcggt ctgaaaccgc acgtccttgc tcagcatctg cgcgaacggt gtctgctcga
      841 ggaaattgtc ggcgagccgc ttgcgcactc cttcgacgac ggccgcgact tccggatcgg
      901 agccgatgcc accgcgcatg agcgacacgt acgcgcgcga gtgctgcgag atgtgatcga
      961 gatacgcctc gatgccggcg cgcacgcgct cgatcggcgc taggtcggga ggaatggtca
     1021 gcacgcgctg cacgagctct tcggcgatct cgcgcagacc ggcgacgtac agatcgcgct
     1081 tggtcgggaa gtagtgatag agcaggccct tcgagatctt cgcttcccgc gcgaggtcgt
     1141 cgatcgacac gtcgtcatac gaacgatccg agaacgcttt gcgcgcgagc tgcagcagct
     1201 gggcgcgacg ttcgtcgttg tcgagacgga tgcggcgccg tggacgtacc gccgcagcgg
     1261 cacgcagcaa gcgtcgaacg ggtttacggc ctggcactta gcccatcata cacgaccgct
     1321 attgactgtt ggtcaatacg tgactagcct cggtgtatgg cgatcccgac gccgcgaaag
     1381 ctcgcggtcg acctgtcgaa tgttccccgc cactggctgg cgggtaaccc agcggcgaca
     1441 gccatctcga acggcatcaa catgctgttt ccgcacggcg aacggttctt cgtccgctcg
     1501 gttcaccact acctcgacga aatcaaagac cctgacctgc gggcgcaggt caaagcgttc
     1561 ttcaagcaag agggccatca cgccagcgcg cacgacgact tcaacgaagc gctgcgtcgc
     1621 cagggcttcg agatcgatcg gttcttgaag cgctaccagc agatcacgaa tttcgtcgag
     1681 gcgcgcatgc ccgcgaagat caacctcgcc ggcacagccg ccgcggagca tttcacagcg
     1741 atcctcgccg acggcgcgtt caccaaaggc gtgctcgacg cgcttcaccc ctcggtgcaa
     1801 cagctgctcg cgtggcacgc cgccgaagag atcgagcaca aggccgtcgc gtacgacgtg
     1861 ctgcagcaga tcgatccgtc gtacgccgtg cgcgtcgccg gtctcgccta cgccaccgtc
     1921 atgctcggca tgttctgggt gtgggcgacg acgatgttgc tccgccagga gaagctcggc
     1981 tggcgcgaga tcctgcgcca gctcggtgcg atgcggaaaa ccgatccggt gattcgccgc
     2041 gtgttcatcg cgggcattcg ccagtacatt cgccgcgact ttcatccgcg ccagaacgcg
     2101 aacgagcacc tcgccgcgca gtggtttgca gcgcgcggct ttacccttcc cgaggcagca
     2161 tgaatctcaa agaccgagtc gttgtcgtca ccggtgcagg tagtggaatt ggtcgcgcta
     2221 cggcgctcgc attcgccgca gaaggcgcgc gcgtcgcggc gtgcgatgtc gacgagaagc
     2281 ggctcgaatc gctcgcgagc gagctcggcg atcgcaagct gctcgtccgg cgcgtcgacg
     2341 tgtccgaccg gctgtcgatg aagctgtttg ccgacgcggt gcacgagctc gtgcccgcgg
     2401 ccgacgtcat cgtcaacaac gcgggcgtcg ccgtcggcgg gacgttcctc gacaccacac
     2461 tcgacgattg ggattggctg ctcggcgtga acctacgcgg cgtcgtgcac ggctgccact
     2521 acttcattcc gaagatggtc gcgcgcgcca gcggcggtca cgtggtgaac gtgtcgtcga
     2581 tcctcggcat ctatccggcg ccgaacgtca cggcgtacgt cgcgagcaag ttcgccgtgc
     2641 gcggcttctc gcagtcgctg cgcgccgagc tcgcgccgca caagatcggc gtcaccgcga
     2701 tctgtccggg catgatcaac accgcgatcg tcgccgatgg ccggatgtcc ggcaacgttg
     2761 gctcgcgcaa ggacaagatg gtcgcgacgt tccacaagcg tgggttggcg ccggcgaagg
     2821 tcgcagaggc gatcctcgac gcggttcgca gcaatccggc cgtgcgccca gttggcgccg
     2881 acgccatggt gatggctgca ctgatgcgcg tcgcgccgcg aacgttcaat cgtctcgccg
     2941 gtacgatcca gcgccgcttc ggcgtcggct gagctgtcga cgtgcacgtg tcacgtgtca
     3001 cgtgcacgtg cacgtgcacg acgggccgaa actcgttcgc cgacatccgg cgacatcgag
     3061 caggtcgtat cccggcatgc ctcgagcagt cgttgcccgg aaccgctcga cttgctccga
     3121 gcaaggtcgc gccggggaat cgctccgatc cggaccgccg gcaaatccga accgtcggcc
     3181 aggcgacgcc gcgtccgcta ggcagcccgc gattcgtgca cgagcacgag cacgagtacg
     3241 tgtacgtgca cggggaaggc gcgctcggct ttgctagtct tccgcgcatg cccaaggctg
     3301 cgccgaaatt cgtcggcttc gatcgcgatg cgctgcagtt cctccacgag ctgacgctcg
     3361 agatgaaccg cgagtggttc gaggcgaaca aagctcgcta ccaggcgcgc tgggtcgagc
     3421 cgatgaccgc gttgctcggc gaggtcgcgg ggcggctcgc gaaggtgtac gcgccgatca
     3481 agctcggccc gccgaagctg ttccggatct atcgcgacac gcggttctcg aaggacaaat
     3541 ctccgtacaa gacgcacgtc gccggcgtga tcccgctgcg cgagggctgc accgcgatgt
     3601 acatgcacat cggcatcgac gacgagttcg tcggcgttgg cacgtactac ttcgaggact
     3661 ctcagctccc gcgctggcgc aagctcgtcg ccgctgacaa gaccggaaaa gagatcgcag
     3721 cgatcgtgac caagttgcgc aaagccgggt acaaggtcgg tggccacgag gactacaaga
     3781 aggtcccaaa gggcttcgac ccggagcatc cgcgcgcgga gtttctgaag atgcgcggcc
     3841 tcaccggcgg atttcccgcg attcccaagg gcctcttgca caagcccgcg cttgccgatt
     3901 ggctgttcac ccacgccaaa gccaccgcgc cgatggtgag ctggctgtac cgcaacgtga
     3961 agtagagctc cgtgacgacc gcgtgaagca gggttctgtg acgccgcgcg gcgtgtgcgg
     4021 ctggtgcttc atccgcgatt tcctgggtct cttggtttgt ccgggtcctt cggataaggt
     4081 gccgtcgacg atgaagcttg cgaccttgcg cgatggctct cgagacggac gactcctcgt
     4141 cgtgcgacgt gatggcgaag ccggtgcgcc ggcgccggat gcctggccga cgctgcaacg
     4201 cgcgctcgat gattgggatg cggcggagcc cgcgctgcgt gcgctccaag atcagctcga
     4261 tcgcggtgcg ctcgatggcg tgacgatcga tccgaaccgg ctcggtgcgc cgctcacgcg
     4321 cgcgtacgaa tgggtcgatg gctcggcgtt cctcaatcac gtgatcctgg tgcgcaaggc
     4381 acgcggtgcc gagccaccga agacgctcga gaccgacccg ctcatctatc aaggtggctc
     4441 gggcgacatg ctcggcccgc gcgatccgat cgagctgcac gagccggcgt ggggcctcga
     4501 tttcgaatcc gaggtctgcg tcgtgctcgg cgatacgccg atcggcacgc gagcggccga
     4561 tgcgcaccac cacgtgcggc tcctcatgct cgcgaacgac tgcacgctgc gcaacctgat
     4621 ccccgaggag ctcgcgaagg gcttcggctt ctttcagagc aaacccgcga ccgcgttcgg
     4681 tccgtttgcg atcacgccgg acgagctcgg cgatgcgtgg cgcggtggca aggcgcacat
     4741 ccgcgtgcgc tcggtctaca acgaggcgca ggtcggcgac tgcgaagccg gtcccgagat
     4801 gcacttctcg ttcttcgatc tcatccagtt catcgcgaag acgcgccggt tcaccgcggg
     4861 cacgattctc ggcagcggca cggtgtcgaa cgctgaccgc agccgcggca tctcgtgtct
     4921 tgccgagcgc cggatgatcg agacgatcga gggcggtaag ccgctgacgc cgttcatgtc
     4981 agtcggcgat cacatcgaga tcgaagcgat cgatccggcg ggtgcgagcc cgttcggcaa
     5041 gatcgatcaa cgcgtcgtcg ctgcgggagg ttcgcgatga agctcgtgct ccacaactac
     5101 tggcgctcgt cggccagcca gcgcgttcgc atcggtctcg gcctcaagca gctcgcgtac
     5161 gattacgtcg tcgtcaacat cctccacaag gaccagcacg ccgacgccta tcgcgcgcgc
     5221 aatccgatgg cgcaggtgcc gacgctcgag atcaccgaag acaacggcac ggtgcacgca
     5281 ctgacgcagt cgctgccgat cctcgagtat ctcgacgagc ggttccccga gaagccgatc
     5341 atgccgaggg atccgtatct gcgcgcccgc acgcgcgcgc tcgccgaaat cgtcaactcc
     5401 ggcatccagc cactgcagaa cctgacgacg acgaaagccg tcaaagcgtt cggtggcgac
     5461 gaggtggcgt ggccgaagga cttcatcgcg aatggcctcg ctgcgttcga ccgcgccgcg
     5521 agcgacatcg cgggcaagtt ctgcgtcggt gatgcgccga cgatcgcgga ctgctgcctc
     5581 atcccgcagc tcacgtctgc gcgccggttt ggcgtcgaca tctcgaagtt cacgttgctg
     5641 ctcggcatcg aagagcgttg tctcgcgatg cccgcgttca cgaatgctgc accggaccgc
     5701 caacctgacg cggtgaagtg aggctgccat ggcaaagcaa gaatccctcg gcatcaagcg
     5761 gctcgaaggc atccactact acgtccacga cctcgaccgc tcacggcagt tctactgcgg
     5821 caagctcgac ttcgcggaga cctggcggtc gagccccgag ctcgagaaga ctgggcgtca
     5881 gaagtccgcg tgcttctccg ctggcaacat caacgtcgtg tgctcgacgc cgaccggtgg
     5941 tggtggcggc ggccgcgccg cacgcttcct gtcgaagcat cccgacggcg tcggcacgct
     6001 gatcttcgag gtcgaggacg ccgagaagac gtttcgcctg ctcgaggagc gcggcggcac
     6061 gccgatcgag gacatccaga cgttcaagga cgacagcggc ggcacgctgc gccagttctc
     6121 gatcacgacg ccgttcggcg actgcacgtt ccgcttccgc gagcggcgcg gcttcaaggc
     6181 gctgtatccg ggcgcggtcg cgcatgacgg cggtggcaag aacaagttcg gcttccagga
     6241 gatcgatcac gtcacgtcga acttccagac catgaagccg atgctgctct ggctcgagca
     6301 cgtgctcggt ttcgagcaga tgtgggaagt cgagttccac accagcgacg tcgatccgaa
     6361 ccgcaagacc ggctcgggcc tcaagtcgat cgtgatgtgg gatccgcagt cgaacacgaa
     6421 gttcgcgaac aacgagccgt cgcgcccgtt cttcaagagc tcgcagatca acatcttcaa
     6481 cgaagagctg cgcggtgatg gcgtgcagca cgtcgcaatg acggtcgccg acatcgtctc
     6541 ggcggtgcgt gcgctgcgcg gcaatggcgt cacgttcatg ccgacgcctg gcacgtacta
     6601 cgacatgctg ccggaacgga tcacgcagag cggcatcaag cagatcgacg aggacctcaa
     6661 gatcctccgc gagctcgaga tcctcgtcga cggcgatcac ttccacgcgt acatgctgca
     6721 gatcttcctc aaggaagcgt ccggcctcta cggcgatccg aaggcgggtc cgttcttcta
     6781 cgagatcatc cagcgcaagg gcgaccgcgg cttcggcgcc ggtaacttcc gcgcgctgtt
     6841 cgaatcgatc gagcgcgagc aacgcgagcg aggcctcatc taatgctcga tcggatgcag
     6901 gtcggcgagg tcgcgaagaa gcaccacatc cagctgcgcg gcaccgacgg cgagctgcgc
     6961 ttcgaagaat gcttcacgcg cgacggcttc gacggtccgt acacgatcat gtatcacctg
     7021 cgccgcccgc acacgcagcg catcgcgccg gccgcccacg gttgggccgc accggtagcc
     7081 gtcaccgagc gcccgctcgc gaagcgtcac tacaagaccg gcgacctcgc gaagcagggc
     7141 agtggcgcgc aggtcgacgc gcgcatcccg ctcgtgttca acgccgacgt cacctgcggc
     7201 gtcgcgttcc cgacggccga agatccggtc tacgtcgtca acggcgatgc ggac
//
LOCUS       DASUFX010000065         9777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_428015,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000065 DASUFX010000000
VERSION     DASUFX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428015"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFV99_02360"
     CDS             <1..355
                     /locus_tag="VFV99_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058169.1"
                     /translation="DDHSRGRTWTNPLDDEAVQTRLARALGGVAPAVPPAAEKPAKKP
                     AAKPAKPAARAKAKAAEKPAVKAPAKKPKAATKPKTTAKAAAQAKPAAKKPKAAAKPK
                     AKAKAKSQTRPKKKR"
     gene            618..1046
                     /locus_tag="VFV99_02365"
     CDS             618..1046
                     /locus_tag="VFV99_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5058170.1"
                     /translation="MGETLQFMQRMWDFVHALDVRSKRMMTTIGVTGPQRLVIRLVGQ
                     KPGQTASDIASMLGKHPSTLTGVLARLEERKMILREVDPEDRRRARFKLTAAGRKIDG
                     QRRGTVEAATRRALGRVKPADIETTLDLVAKLVEELERPD"
     gene            complement(1043..2083)
                     /locus_tag="VFV99_02370"
     CDS             complement(1043..2083)
                     /locus_tag="VFV99_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5058171.1"
                     /translation="MRILGASLVLLAACGNSGQDSGGGGGGGDGGDDDDVAMPDAPAP
                     PKPVKFIAVGDTGKGNADQRRVAIAMRDLCAAKGCDFVLMLGDNIYDAGVTSTTDSQW
                     QTKFEQPYMDVNLPFYVALGNHDYGGKLLIDIAGIGNEFDKGKVEVDYTQVSMKWNMP
                     ATHYTFTYGDVGFIVLDTNSILWSDTTYGDQAAWLPSALAEVSGKRWVFTAGHHPYLS
                     NGQHGNAGDYDAPELAGISIPNPLPIQNGAALKSFFDQNVCGISQAYICGHDHSRQWI
                     NEPTALCGTEMIISGAGSSVSSLQDRGNQSFYEDATKVGFMYVVIDGDKFTGQFYDAD
                     GALDYERTFMHP"
     gene            complement(2130..4337)
                     /locus_tag="VFV99_02375"
     CDS             complement(2130..4337)
                     /locus_tag="VFV99_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058172.1"
                     /translation="MGSPENSPPDRPRTVPEDARWDPKDPGFEWIAGGLDADGRRHGT
                     YRSWTRDGVLHGESNYVHGKVHGKNLNYHPDGTVASEADWVMGLILDSVFFRSDAPTP
                     EPFAQAAPNVWSVRYYTKDGKTNYTIRYFLRDGTECGPDGNLLPPRPANVSPDARWFP
                     DMDRWVDGSIERGTNNQVGHWRWWSREGTLRHEEMRDSSGQAALVAQYESDGALKKKT
                     TRNAQGEERDYYFDGGKLSTRYREDAKGRQTYKGSWLRDGELDEESTRTFENDTLASV
                     TERGRGGMLRFEARREGPALACVLYHDDGKTISATGVIESEKLVGTWRIFDAAGAVRR
                     EVDMTPLEIQQRPTAEGLSWELGHALYKTDEPSLPTPEQLVGVDDEPWGEPSGCFDEN
                     VEEFPRLLRGLTSRDELIRQYCLGAIDHEIEHQGSTYAATARVIPWLAKLLSHPDVDR
                     PTLLATIQSAGENAAPYFDQVQELDADDPDRIAIEGTANAVSAAWPQIFAVFPHATPA
                     ERRTILVLAKFASDAKPNIIEVARRDADPGMRACAIDSLTSLPGYDLADVTPCLTDKD
                     ALVRAATAIAIALSKGPDAPRDVVAVLRDAVHSYKDIASRWQELPYTDGHVLAYLALA
                     TGAVRSSDARSLAQALCERIDDVDGRSAVTYGHGLLALALGSGERPFAKRFIEILDTL
                     ATSKQFWVFNVNASEVLEKWNLPRSHDALVALVAELKATPDPEAWLHAKMHAE"
     gene            4381..4926
                     /locus_tag="VFV99_02380"
     CDS             4381..4926
                     /locus_tag="VFV99_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5058173.1"
                     /translation="MGYDWVMSEPPPLDPETLHDLELLAAAIAQREDHADLARRFEYL
                     VDTLIFRGQLPESFRRVLKKISDKRSLVRLATFRDKYQVESADIDCASRIPLCKARCC
                     SMEVTLSAQDVVEGGIPFQITEPYAMPRDAATKQCVCMDAAGACTIYDKRPGACRAYD
                     CRNDPRVWIDFEARIATPLPE"
     gene            complement(4915..5616)
                     /locus_tag="VFV99_02385"
     CDS             complement(4915..5616)
                     /locus_tag="VFV99_02385"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5058174.1"
                     /translation="MRALVLSCEHASWTLPPGIDLGVPPEVLQSQAGWDHGAFDIASR
                     LSEGVGLPVHTGAFTRMFVDLNRAPDHPDVIPAVSYGEPVPGNAHLSDGDRKARIELF
                     HAPYWQAVRRDVHARLHDTGEVLHLSSHSFAPELDPTNRTYDVGVLYDPSHAYEAEIA
                     ERLMFELRGAGINVRANQPYSGTGPAICTSLREELTGQRYAGIELETSHAVTRATGGC
                     ARVAAAVIRFLELLR"
     gene            complement(5717..6550)
                     /locus_tag="VFV99_02390"
     CDS             complement(5717..6550)
                     /locus_tag="VFV99_02390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5058175.1"
                     /translation="MRTALLLALIACSSPSKPATTTTTTAPTTNVPASTGPLTEEQFK
                     ALHASPTENKAPLHGTQIDLDGTKAYLSVPEGKGPFPGIVVIHEWWGLNENIEHWADR
                     LATAGYAALAVDLYGGVVATTPDDAMKAVKSVDKQKAIGIIKTAFDFLASDSRIMATK
                     RAVIGWCFGGGYSLQAALDIPQLDGAIIYYGQLETDPQKLATIKAKLLGIFGNRDKGI
                     PPEKVAEFDAALKKANVRHEIFQYDAEHAFANPSNPKYDEKSAADAWTHVLAFLDSLK
                     S"
     gene            complement(6563..7270)
                     /locus_tag="VFV99_02395"
     CDS             complement(6563..7270)
                     /locus_tag="VFV99_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEX5058176.1"
                     /translation="MSDNDDKVLDELAAAPPPAPPPMSAELQAELGSLTPVAPRRPLR
                     QLAIVVAVSLLYGAGLIAALSMRRDMDELPMGWLVATAIAWLVGFALPCYLALVPRKG
                     SMMPRTRWAAASAIVASIAFIALGLTVHPHGPSSLDYSQQFAHGHWCLEIGLATALVP
                     VIAGAVFLRGALPVGSRWIAAALGAGGGCLGGLVLHLHCRIADRWHVGLIHGGVVLVA
                     ALLSAALVPRATDRPLT"
     gene            complement(7276..7863)
                     /locus_tag="VFV99_02400"
     CDS             complement(7276..7863)
                     /locus_tag="VFV99_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5058177.1"
                     /translation="MADPRDLMARYQDGDANAFRELYMLVAPRLLGYLMKMTRSKAVA
                     DDLLQQTFLKIHRARAAYVRGADPLPWIYSIAHRTFIDETRRQKRAVVRVADDEVPEV
                     SADLSGVATEHRDDPIDPELTRAALDALAQLPEQQREAVVLTKLDGKTVAEAAEIAGT
                     SVGAMKVRAHRGYEALRKLLGGKPAAARKAAGGSL"
     gene            7936..8091
                     /locus_tag="VFV99_02405"
     CDS             7936..8091
                     /locus_tag="VFV99_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058178.1"
                     /translation="MGRRTKRSVKNRPKFKQKRRSKDRRMKAKKSAAHKRGLARNKAR
                     RRRRGKK"
     gene            complement(8101..8781)
                     /locus_tag="VFV99_02410"
     CDS             complement(8101..8781)
                     /locus_tag="VFV99_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEX5058179.1"
                     /translation="MTPLVIPTAVSALACLVLVAAEYKHWRVVRAIAKPIASFAFIAV
                     AVMTLTSCVPHASSWSEYQLWVIVGLVLGTAGDIALLGKSQGAFLVGLVAFLFGHIAY
                     VVAFAQLAAPSTWLDVRAVIPLAIGGTALRLLWPRLGDMRVPVIAYVLTIVTMVTAAI
                     AAARHAVLPEQNRMLLLAGAALFFVSDLAVARDKFVGASFINRAWGLPAYYAGQLLIA
                     WSLWPSFC"
     gene            complement(8778..>9777)
                     /locus_tag="VFV99_02415"
     CDS             complement(8778..>9777)
                     /locus_tag="VFV99_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5058180.1"
                     /translation="LPHSRWPAAIDAIYAAYWQRAEDITRDEVIAAALRGAGVSDPDI
                     TDAQSRADAMKDDLRARTDEAIALGIFGAPAWVVRRDGQAPLLIWGQDRMPWVDAALA
                     GWDPETSAPPAGARGFGDGMQGFVTTSSLDVFCDVSSPFSYLGLMQLPRLAELTGVTP
                     RLQPILLGALFRDIGQVNVPLLAMPPPKMRYVGLEMTRWARWWGVPFDMPKKFPQRTI
                     TAQRLILLATERGFPDAIRLANTLGRAMWAEQKNLEDDDTLRGLLADAGLPREWFERT
                     QEPAVKAALADRTARAQAAGVFGVPTWIVDEKYLFWGQDRLELVMRALGGWRPVHG"
BASE COUNT         1667 a         3189 c         3293 g         1628 t
ORIGIN      
        1 cgacgatcat tcgcgcggcc ggacgtggac gaacccgctc gatgacgagg ccgtgcaaac
       61 ccggctggca cgcgcgctcg gtggtgtcgc gccggcggtg ccgcccgccg cagagaagcc
      121 ggcgaagaag ccggccgcga aaccggcaaa gccggcggca agagccaagg ccaaagctgc
      181 cgagaagccc gccgtgaaag ctcccgcgaa gaagcccaag gccgcgacca agccgaagac
      241 aacggcgaag gcggcagcgc aggcgaagcc ggccgcgaag aagcccaaag cggcggccaa
      301 gccgaaggcc aaggccaagg ccaagtccca gacaagaccc aagaagaagc gctgagcggg
      361 tctcgccgga cccgcaccgc acgcgaatcc ggcgaaacta ctcggccgtt tcctggcaaa
      421 ccaggtgcag tccctgcgcg cgacatgacg cgcttcgatc gcttctggga accgctctgc
      481 ttcgtggttg cgttcgggat cgtatggatc ggaacgctgt gattcgcgcg tgaaatgctt
      541 tgggttgcaa ttgacggcta ccggcgccgg gccctagtgt gcgcggcaaa atggcgacgc
      601 cgtcgaagcc tcagccgatg ggtgagacgc tgcagttcat gcagcgaatg tgggatttcg
      661 tgcatgcgct cgacgttcgc tccaagcgga tgatgacgac catcggcgtc accgggccgc
      721 agcgcctcgt gatccgactg gtagggcaga agcccggcca gaccgcgagc gacatcgcgt
      781 cgatgctcgg caagcacccg agcacgctga ctggtgtgct cgcgcggctc gaggagcgca
      841 agatgatcct gcgcgaggtc gatccggagg atcgtcgccg cgcgcgcttc aagctgacgg
      901 ccgcaggcag gaagatcgac ggccagcgcc gcggcacggt cgaagcggcc acccggcgcg
      961 cactcggtcg cgtgaagccg gccgatatcg agaccacgct cgacctggtc gccaagctcg
     1021 tcgaagagct cgagcgcccg gactaaggat gcatgaaggt gcgctcgtaa tcgagcgcgc
     1081 cgtcggcgtc gtagaactga ccggtgaact tgtcgccgtc gatcacgacg tacatgaagc
     1141 cgaccttggt cgcgtcctcg tagaacgact ggttgccgcg atcctggagc gacgacacgg
     1201 acgagcctgc gccgctgatg atcatctcgg tgccgcacag tgcggtcggc tcgttgatcc
     1261 actggcggct atgatcgtgg ccgcagatgt acgcctggct gatgccgcag acgttttggt
     1321 cgaagaacga cttcagcgcg gcgccgttct ggatcggtag cggattgggg atcgagatgc
     1381 cggcaagctc gggcgcgtcg tagtcaccgg cgttgccatg ctgaccgttg ctgagatacg
     1441 gatgatggcc ggccgtgaac acccagcgct tgccggagac ctcggcgagc gcgctcggta
     1501 gccacgccgc ttgatcgccg tacgtcgtgt cgctccacag gatcgagttc gtgtcgagca
     1561 cgatgaagcc gacgtcgccg tacgtgaacg tgtagtgcgt cgcaggcatg ttccacttca
     1621 tcgagacctg cgtgtagtcg acctcgactt tgcccttgtc gaactcgttg ccgatgccgg
     1681 cgatgtcgat caagagcttg ccgccgtagt cgtggttgcc gagcgcgacg tagaacggca
     1741 ggttgacgtc catgtacggc tgctcgaact tcgtctgcca ctgagagtcg gtcgtcgacg
     1801 tgacgcccgc gtcgtagatg ttgtcgccga gcatgagcac gaagtcgcag cccttcgcgg
     1861 cgcacaggtc gcgcatcgcg atcgcgacgc ggcgttgatc cgcgttgcct ttgccggtat
     1921 cgccgaccgc gatgaacttg accggcttcg gcggcgccgg ggcatcaggc atcgcgacgt
     1981 cgtcgtcgtc accaccgtca ccgccaccgc caccaccacc gctgtcctgg ccgctattgc
     2041 cgcaggcagc gagcaacacg aggcttgccc caaggatccg catcgcggcg gaacctaacg
     2101 caggtcggaa ccgaaccgat cgagaaacgt tattcggcgt gcatcttggc gtgaagccac
     2161 gcctcgggat cgggcgtggc cttcagctcg gcgacgagcg ccacgagcgc gtcgtgcgag
     2221 cggggcaggt tccacttttc gaggacttca ctcgcgttga cgttgaacac ccagaactgt
     2281 ttgctggtcg cgagcgtgtc gaggatctcg atgaagcgct tcgcaaacgg ccgttccccg
     2341 ctgccgaggg cgagcgcgag caggccgtgt ccgtaggtca ccgcagagcg accatcgacg
     2401 tcgtcgatac gctcgcacag cgcctgggcg agcgagcgcg catcagatga gcgaaccgcc
     2461 ccggtcgcga gcgcgagata ggcgagcacg tgaccgtctg tgtacggcag ctcctgccag
     2521 cgcgacgcga tgtccttgta ggaatgcacg gcatcgcgca gcaccgccac gacgtcgcga
     2581 ggcgcgtcgg gacctttgct gagcgcgatc gcgatcgcag tcgcagcgcg cacgagcgcg
     2641 tctttgtccg tgaggcaggg cgtaacgtcg gcgaggtcgt agccgggcag cgacgtcaga
     2701 ctgtcgatcg cgcatgcccg catgccggga tcggcgtcgc ggcgcgccac ctcgatgatg
     2761 ttcggctttg cgtcggaggc gaacttcgcg aggacgagga tggtgcggcg ttcggcgggc
     2821 gtcgcatgcg gaaacaccgc gaagatctgc ggccacgccg cgctcaccgc attggccgtg
     2881 ccctcgatcg cgatgcggtc tggatcgtcg gcgtccagct cttgtacctg atcgaaatac
     2941 ggcgccgcgt tctcgccagc gctctggatc gtcgcgagca gcgtcggccg gtcgacatca
     3001 ggatgcgaca acagcttcgc gagccacggg atcacgcgcg cggtcgctgc ataggtcgag
     3061 ccttgatgct cgatctcgtg gtcgatcgcg ccgagacaat actggcggat gagctcgtcg
     3121 cgcgacgtga ggccacgcag caggcgcgga aactcctcga cgttctcgtc gaagcaaccc
     3181 gacggttcgc cccacggctc gtcgtcgact ccgacgagct gctcgggggt gggcagcgac
     3241 ggctcgtcgg tcttgtacag cgcgtgaccg agctcccacg acagaccttc cgccgtcggc
     3301 cgctgctgga tctcgagcgg cgtcatgtcg acctcgcggc ggacggcgcc cgctgcatcg
     3361 aagatgcgcc aggtgccgac gagcttctcg ctctcgatga cgcccgtcgc tgagatcgtt
     3421 ttgccgtcgt cgtgatagag cacgcaggcg agggcagggc cttcgcgtcg ggcctcgaac
     3481 cgcaacatgc cgccgcgacc gcgctcggtc acgcttgcca gcgtgtcatt ctcgaacgtg
     3541 cgcgtgcttt cctcgtcgag ctcgccgtcg cggagccagc tgcctttata cgtttgtcgc
     3601 cccttcgcgt cctcgcgata gcgcgtcgat agcttgccgc cgtcgaagta gtagtcgcgt
     3661 tcttcaccct gcgcgttgcg tgtcgtcttc ttcttgagag cgccgtcgct ctcgtactgc
     3721 gcgacgagcg cggcctggcc gctcgagtcc cgcatctcct cgtggcgcag cgtgccctcg
     3781 cgtgaccacc accgccagtg gccgacctga ttgttcgtgc cgcgctcgat cgagccgtcg
     3841 acccagcgat ccatgtcggg gaaccagcgc gcgtcggggc tcacatttgc aggacgcggc
     3901 ggcagcaggt ttccatcggg accgcactcg gtgccgtcgc gtaggaagta gcgaatcgtg
     3961 tagttcgtct tgccgtcttt cgtgtagtag cgaaccgacc acacgttcgg cgcggcttgc
     4021 gcgaatggct ccggggttgg cgcgtcgctg cgaaagaaca cgctgtcgag gatgaggccc
     4081 atcacccaat cggcttcgga cgcgaccgtg ccatcgggat ggtagttgag gttcttgccg
     4141 tgcactttgc cgtgcacgta gttgctctcg ccgtgcagaa cgccatcgcg cgtccagctg
     4201 cggtacgtgc cgtgacgacg tccatccgcg tcgaggccgc ctgcgatcca ctcgaagccc
     4261 ggatctttgg ggtcccagcg cgcatcctcc gggaccgtgc gcgggcggtc ggggggtgag
     4321 ttctccgggc tccccatctc cgacgatcct gatcgtgtga cctcccgaga tcaatgagac
     4381 atgggttacg actgggtcat gtcggagccg ccaccgctcg accccgagac gctgcacgat
     4441 ctcgagctgc tagcggctgc catcgcacag cgcgaagacc acgctgatct cgcgcggcgc
     4501 ttcgaatacc tcgtcgatac gctgattttt cgcggtcagc tacccgagtc gtttcggcgc
     4561 gtgctgaaaa agatcagtga caagcgatcg ctcgtgcgcc tcgcgacgtt tcgcgacaag
     4621 taccaggtcg agagcgctga catcgactgc gcatcgcgga tcccgctctg caaggcgcgg
     4681 tgttgctcga tggaggtcac cctatcggcg caagacgtgg tcgagggcgg cattccgttc
     4741 cagatcacgg agccgtacgc gatgccccgc gacgcggcga cgaagcagtg cgtatgcatg
     4801 gatgcggccg gcgcgtgcac gatctacgac aagcgcccgg gcgcgtgtcg cgcgtacgac
     4861 tgccggaacg atccgcgcgt gtggatcgac ttcgaggcgc gaatcgcgac gccgctaccg
     4921 gagtagctcg aggaagcgaa tcaccgcggc cgcgacacgt gcacagccgc cggtcgcgcg
     4981 cgtcaccgcg tgcgaggtct cgagctcgat accggcgtag cgctggccgg taagctcctc
     5041 gcgcagcgac gtgcagatcg ccgggccggt gccgctgtac ggctggttcg cgcgcacgtt
     5101 gataccggcg ccgcgcagct cgaacatcag ccgctcggcg atttccgcct cgtacgcgtg
     5161 cgacggatcg tagagcacgc cgacgtcgta cgtgcgattc gtcggatcga gctcgggcgc
     5221 gaagctgtgc gacgacaggt gcagcacttc acccgtatcg tgcaggcgcg cgtggacgtc
     5281 gcggcgcacg gcttgccagt acggcgcatg gaacagctcg atgcgtgcct tgcgatcgcc
     5341 gtcggagagg tgcgcgttgc cgggcacggg ctcgccgtag ctgaccgctg gaatcacgtc
     5401 gggatgatca ggcgcgcggt tgagatcgac gaacatccgc gtgaacgcgc cggtgtgcac
     5461 cggcaggccg accccctccg acaaccgcga tgcgatatcg aacgcgccgt ggtcccagcc
     5521 cgcttgcgac tgcaagacct ccggcggaac tcccagatcg attccaggcg gcaacgtcca
     5581 cgatgcgtgc tcacacgaca gcactaacgc gcgcactact ctcaacggta aaagcctctg
     5641 cctttggcgt cgaggaagcg accgggtagc gatgccttcc tgatcgggaa gcgatcggat
     5701 agccgcgaga tcgtcttcac gatttcagac tgtcgagaaa tgcgagtacg tgtgtccatg
     5761 catccgccgc cgacttctcg tcgtacttcg gattcgaggg gttcgcaaat gcatgctccg
     5821 cgtcgtattg gaaaatctcg tggcggacgt tcgctttctt gagcgcggcg tcgaactccg
     5881 cgactttctc cggcggaata cccttgtcgc ggttaccgaa gatgccgagc agcttcgcct
     5941 tgatcgtcgc gagcttctgc ggatcggtct cgagctggcc gtaatagatg atggcgccgt
     6001 cgagctgcgg aatgtcgagc gctgcctgta gcgagtagcc gccaccaaaa caccagccga
     6061 tgaccgcgcg tttcgtcgcc atgatgcgtg aatcgctggc gagaaagtcg aacgcggtct
     6121 tgatgatgcc gatggccttc tgcttgtcga cggacttcac cgccttcatg gcgtcgtctg
     6181 gagtcgtcgc gacgacgccg ccatacagat cgacggcgag cgcggcatag ccggcggttg
     6241 caagccggtc tgcccagtgc tcgatgttct cgttgagtcc ccaccactcg tggatcacga
     6301 cgatgcccgg gaacggtcct ttgccctcgg gcacgctcag gtaggccttc gtgccgtcga
     6361 gatcgatctg cgtgccgtgc agcggcgctt tgttctcggt tggtgacgcg tgcaacgctt
     6421 tgaactgttc ctcggtgagc gggccagtcg acgccggcac attggtggtc ggtgcggtgg
     6481 ttgtcgtggt cgtcgccggc ttgctcggcg agctgcatgc gatgagcgcg agaagaagag
     6541 cggtacgcat gcccgcgctt tatcacgtca gcgggcgatc ggtagcgcgt ggcaccagcg
     6601 ctgctgataa caatgcggcg accagcacga cgccgccgtg aatcaggccg acgtgccagc
     6661 gatctgcaat gcggcagtgc aagtgaagca cgaggccgcc gagacagccg ccgccggcac
     6721 cgagtgcggc tgcgatccag cgcgagccga ccggcaacgc gccgcggagg aataccgcgc
     6781 cggcgatcac cggcacgagc gccgtcgcga gcccgatctc gagacaccag tgaccgtgtg
     6841 cgaactgttg cgagtagtcc aagctgctcg gtccatgtgg atggacggtg agtccgagcg
     6901 cgatgaacgc gatcgacgcg acgatcgcgc tcgccgccgc ccaccgcgtg cgcggcatca
     6961 tcgacccctt gcgcggcacg agcgcgaggt aacacggcag cgcaaagccg acgagccatg
     7021 cgatcgcggt cgcgacgagc cagcccattg gtagctcgtc catgtcacgg cgcatgctga
     7081 gcgcggcgat gagcccggcg ccgtagagga gcgagacagc gacgacgatc gcgagctggc
     7141 gcagcggacg ccgcggcgcg acgggcgtga gcgagccgag ctcggcttgc agctccgccg
     7201 acatcggcgg tggtgcgggc ggcggcgcag cagcgagctc gtcgagcacc ttgtcgtcgt
     7261 tgtcgctcac ggcggtcaca ggctccctcc cgctgccttg cgcgcggccg cgggcttgcc
     7321 gccgagcagc ttgcgcaacg cttcgtagcc gcgatgcgcg cggaccttca tcgcaccgac
     7381 cgatgtgccg gcgatctcgg ccgcctcggc gacggtcttg ccgtcgagct tcgtgagcac
     7441 gactgcttcg cgttgttgct ccggcaactg cgcgagcgca tcgagtgcag cgcgcgtgag
     7501 ctcgggatcg atcggatcgt cgcggtgctc ggtcgccacg cctgatagat ctgccgatac
     7561 ctcgggcacc tcatcatctg cgacccgcac gaccgcgcgc ttctgcctgc gcgtctcgtc
     7621 gatgaacgtg cggtgcgcga tcgaatagat ccatggcagc ggatcggctc ctcgcacgta
     7681 cgcggcgcgt gcgcggtgga tcttcagaaa cgtctgctgc aggaggtcgt cggcgaccgc
     7741 cttgctgcgc gtcattttca tcaggtagcc gagcaggcgc ggggcaacga gcatgtagag
     7801 ctcgcgaaag gcattcgcgt cgccatcctg gtaccgcgcc atcaaatccc tgggatcggc
     7861 cattcgaccg gacctgggta tacgacggcc acgccctctg gttacaggtc ggcagagcga
     7921 tgtatagacg cggacatggg tcgtcgtacc aagcgaagtg tcaagaaccg tccgaagttc
     7981 aaacagaagc gccgcagcaa ggatcgccgg atgaaggcga agaagtccgc tgcgcacaag
     8041 cgcggcctcg cgcgcaacaa ggcgcgccgc cgtcgccgcg gtaagaagta atacggtcgt
     8101 tcagcagaag ctcggccaca gcgaccacgc gatgaggagc tggcctgcgt agtacgcggg
     8161 cagcccccat gcgcgattga tgaagctcgc tccgacgaac ttgtcgcgcg cgaccgcgag
     8221 gtcgctcacg aagaacaggg cagcgccggc gagcagcagc atgcgatttt gctccggcaa
     8281 caccgcgtgg cgggctgccg cgatggcggc ggtcaccatc gtgacgatcg tcagcacgta
     8341 cgcgatcacc ggtacgcgca tgtcgccgag ccgcggccac agcaggcgga gtgccgtgcc
     8401 gccgatcgcg agcgggatca cggcgcggac gtcgagccac gtcgacggtg ccgcgagctg
     8461 cgcgaacgcg acgacgtacg cgatgtgacc gaacaggaac gcgacgagcc cgacgaggaa
     8521 ggcaccctgg ctctttccga gtagcgcgat gtcgccggcg gtcccgagga cgaggccgac
     8581 gatcacccag agctggtact cgctccacga cgaggcgtgc ggcacgcagc tcgtcagcgt
     8641 catgacggcg accgcgatga atgcgaacga cgcgatcggt tttgcgatcg cgcgcacgac
     8701 acgccagtgc ttgtactcgg cggcgacgag tacgaggcac gcgagcgcgg agaccgctgt
     8761 cgggatcacg agcggcgtca tccgtgaacc ggtctccaac caccgagcgc gcgcatcacg
     8821 agctcgagtc ggtcttgtcc ccagaacaga tatttctcgt cgacgatcca cgtcggcacg
     8881 ccgaatacgc ctgccgcctg cgcacgcgca gtgcggtcgg cgagcgccgc tttgacggcg
     8941 ggctcctgcg tgcgctcgaa ccactcgcgt ggcagaccgg cgtcggcgag caatccgcgc
     9001 agcgtgtcgt cgtcctcgag gtttttctgc tcggcccaca tcgcgcggcc cagcgtgttc
     9061 gcgagtcgga tcgcgtcggg gaagccgcgt tcggtcgcga gcaggatcaa gcgctgtgcg
     9121 gtgatcgtgc gctgcggaaa cttcttcggc atgtcgaacg gcacgcccca ccagcgtgcc
     9181 caccgcgtca tctcgagccc gacgtagcgc atcttcggcg gtggcatcgc caacagcggc
     9241 acgttcacct gaccgatgtc gcgaaacagc gcgccgagca ggatcggctg caagcgcggc
     9301 gtcacgccgg tcagctcggc gagccgcggc agttgcatga ggccgaggta cgagaacggg
     9361 ctcgacacgt cgcaaaagac gtcgagcgat gacgttgtca cgaagccctg catgccatcg
     9421 ccgaacccac gcgcgcctgc aggcggtgcg ctggtctcgg gatcccaacc ggcgagcgcc
     9481 gcgtcgaccc acggcatccg atcctggccc cagatcaaga gcggcgcctg gccgtcgcgg
     9541 cgaacgaccc aggccggcgc accgaagatg cccagggcaa tcgcctcgtc ggtgcgggcc
     9601 cgcaggtcat ctttcatggc gtccgcccgg ctctgcgcgt cggtgatgtc ggggtcgctc
     9661 acacctgctc cgcggagcgc ggcggcaatg acctcgtcgc gggtgatgtc ctcggcgcgc
     9721 tgccaataag ccgcgtagat cgcatcgatg gcggccggcc accgggagtg ggggagg
//
LOCUS       DASUFX010000066         6093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_983995,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000066 DASUFX010000000
VERSION     DASUFX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983995"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFV99_02420"
     CDS             <1..599
                     /locus_tag="VFV99_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058181.1"
                     /translation="AKGLALEALVAVTPAPGKPRLKWLVVSLPAGADRAMTHLPGDYA
                     GATLTVVDASPFPRSCPSEGGCVDQLTPPTDIDRDAPQMVSGLTRVSGQDPTVDGSFG
                     VVGVAQLDARAKICVDVAGRVTSIELVQPSASAKYDQRIVETVKQWVFAPYVFAGKSR
                     PACTNASFRAYINNTPGWGDGLPAPKEVPTTPSGPSGP"
     gene            632..1258
                     /locus_tag="VFV99_02425"
     CDS             632..1258
                     /locus_tag="VFV99_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5058182.1"
                     /translation="MSSQLVTSEDVEKTITAFTRLDQRGDKKAMNKLAQRLQKEQPFL
                     LQHAAAVRTEHGDAVGEAAVFYATLVWAMFDRGHEATLPRLTAQNLTDADKIVTEELS
                     KIEGLADKPPHERVAPALVKTQPFIYAKLAELLAEDVKEAAMTADTASTIIKPTEVVI
                     EAFDAALSGRRPGERQGTVVAAEKVGRNEPCPCGSGKKFKKCHGLEAA"
     gene            complement(1263..2066)
                     /locus_tag="VFV99_02430"
     CDS             complement(1263..2066)
                     /locus_tag="VFV99_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058183.1"
                     /translation="MSNVINEANWKFHWRLTESAGIMVYLADYKGRRVLWEGSLPYVT
                     VDHQRASLEVEAEAETRGPWWVPLGTRSLQGPVRVQSFRGGIELSANFAVGPYQYTQL
                     WRFHDDGRLCPWLTIYGPGVHDQHTYHPHFRFDFDLDGADEDAFEYFEDGKWQRVTQE
                     GWFPYSGEADGEGNVWRQIDFGSRASINVRPHSWDDAEVFAIRYHDGEWAPFSPRSAA
                     GAQPFPAAYVGNEELEGQDLSLWYVAHVHFDQSFPYTAGPWIRVEGLAK"
     gene            complement(2066..2743)
                     /locus_tag="VFV99_02435"
     CDS             complement(2066..2743)
                     /locus_tag="VFV99_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058184.1"
                     /translation="MAPSKTAVKAKQPAGKPAAKEAKGKAKPKAEAKAPNGKGKRKPA
                     ADITEADPTLFDPLTPGETADALRTLIEDRRLSSMAKVGRYRVICTEPLVVKPPHWMA
                     GHRLARVVAYDYAADRSVDATVDLDAGTVAHLDITRAQPMLSREEEALAISIAMVDGR
                     VRDKLQMGDAPQSTMHYWGRTPNDLAFARRSAAVIFGRSGGHASLIAVVDLVDQTVTQ
                     VVPAELW"
     gene            2849..3955
                     /locus_tag="VFV99_02440"
     CDS             2849..3955
                     /locus_tag="VFV99_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058185.1"
                     /translation="MRMLLASLLFTAACSSAYADHAAVQVGPRIALAVSPDKVRAPYD
                     VQVITESGETAPTYAFKGRYYVQGNDSEHYTIRVTNPTGNRVEAVVSVDGLDVVDGED
                     GDLNKRGYIVPAYGEVRIEGFRTSLADVASFRFSSVDNSYAGKKGKSRNVGVIAVAIF
                     EEHAPPPEQQIIVPEVTRRPTYHWTDDMDGEASVDAGPAPRDRGSSTGGRVAQHNKQA
                     EKKTAAADRAEAKPSRPAPPPPAAPGGVVGGGGRGAPATATAPAPRRQMEEDFGGDEG
                     YYQPDPTPAPRTNRPGLGTEFGESRYSAASYTKFVRAANRPIAIAELRYNDVTGLVAL
                     GIPVQPAPDEGEIMLRETADPFPGDNRFSRPPGR"
     gene            complement(4125..6011)
                     /locus_tag="VFV99_02445"
     CDS             complement(4125..6011)
                     /locus_tag="VFV99_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058186.1"
                     /translation="MSFRCPVCQRSFAEPGFCPFDGKPLVQVSAAEQQTVLSAQHAAQ
                     SPTEAPDTDPIVESPHVDKLQTKPGLGDNTPNKLKALSAHEGHAEALETIRKRVSEYD
                     KLIGETLDGRYYVQRKIGEGGMGVVFAVKHAVIERPLAIKVLKREVMRDAAVIQRFIQ
                     EAKAASRIGHPNIVDVTDFGKTSEGMTYSVMEYVDGTTLSKVIKSAAPLPAERVVRVA
                     SQIARALAAAHAKGIVHRDLKPENVFLIDRDFRPDFVKIVDFGIAKVQPVDGNPDPTA
                     PRLTRVGSVFGTPEYMAPEQASGRGDTDHRVDIYALGVIMYEMLVGKVPHKGESMIRT
                     IAMQMLEPIIPPSKANPEVRIPPALEAIVMKALEKERENRYDKMSTLLAALEDIAPKL
                     EQLSSLPALPPGSTQAETLRRNKPATRPLHEPEFVAPGAPVSFSHVYDDVPVPEKRRW
                     PLWLAGLLMLLVGAGGMVLVISKLQSHDDEHAEAPHDAARVVELRDAPVEVPADAPVI
                     VDVPPDAGTHVIATVPRDAGVHSVVRPPKGNVTIEVLTRPGEANVYIAPNFRGPSGVK
                     ITEPYGAKRHIECKTDRQKGSVDVVFDGKVTAVMCTATRDRFCVPGLKNPYDDCEEDP
                     NAGP"
BASE COUNT          996 a         2022 c         2005 g         1070 t
ORIGIN      
        1 cggcaaaggg actcgcactc gaagcgctcg tcgccgtcac gcccgcgccg ggcaagccgc
       61 gactcaagtg gctggtcgtc agtctgccgg ccggtgccga ccgcgcgatg actcacctgc
      121 cgggcgatta tgcgggcgcg acgctcaccg tcgtcgacgc cagtccgttc ccacgcagct
      181 gcccgtcgga aggtggttgc gtcgatcagc tgacgccgcc gaccgacatc gatcgcgacg
      241 cgccgcagat ggtttcgggt ctgacgcggg tctcgggcca agaccccacc gtcgacggtt
      301 ccttcggtgt agtcggtgtc gcccagctcg acgcgcgtgc caagatctgc gtcgatgtag
      361 ctggccgcgt gacttcgatc gagctcgtcc agccgagtgc gagcgccaag tacgaccagc
      421 gcatcgtcga gacggtcaag cagtgggtgt tcgcgccgta cgtctttgcc ggtaagtcac
      481 gtccggcctg cacgaatgcg tcgttcaggg cgtatatcaa caacaccccg ggatggggtg
      541 atggcctccc cgcgcccaag gaagtaccga cgactccgtc agggccgtca gggccttgac
      601 gtccgccgct gacaagacaa gatcgctcgc catgtcgtcg cagctcgtca cctcagaaga
      661 cgtcgagaaa acaatcaccg ccttcacgcg cctcgaccag cgcggcgaca agaaggccat
      721 gaacaagctc gctcagcgcc tgcagaagga acagccgttt ctgctccaac acgcggccgc
      781 ggtgcgcacc gagcacggcg acgccgtcgg tgaagccgcc gtcttctacg cgacgctcgt
      841 gtgggcgatg ttcgatcgcg gccacgaagc gacgctgccg cgcctcactg cgcagaatct
      901 caccgacgcg gacaagatcg tcaccgagga gctgtcgaag atcgaaggcc tcgccgacaa
      961 gccgccgcac gagcgcgtcg cgccggcgct cgtgaagacg cagccgttca tctacgcgaa
     1021 gctcgccgag ctgctcgccg aggacgtcaa ggaagcggcg atgacggcgg ataccgcgtc
     1081 gacgatcatc aagccgaccg aggtcgtgat cgaagcattc gatgccgcgc tgtcgggacg
     1141 tcgtcccggc gagcgtcagg gcaccgttgt cgccgccgag aaggtcggcc gcaacgagcc
     1201 gtgtccgtgc ggcagcggca agaagttcaa gaagtgccac ggcctcgagg ccgcgtaacg
     1261 cactacttcg cgaggccttc gacgcggatc cacggccccg cggtgtacgg gaacgattga
     1321 tcgaagtgga cgtgcgccac gtaccacagc gatagatcct gaccctcgag ctcctcgttg
     1381 ccgacgtacg cggccgggaa cggctgtgca ccggcggcgc tgcgcggcga gaacggcgcc
     1441 cactcgccgt cgtgatagcg aatcgcgaac acctcggcgt cgtcccacga gtgcgggcgc
     1501 acgttgatcg aagcgcgcga tccgaaatcg atctggcgcc acacgttgcc ttcgccatcg
     1561 gcttcgcccg agtacgggaa ccagccttcc tgcgtcacgc gctgccactt accgtcctcg
     1621 aagtattcga acgcgtcttc gtcggcgccg tcgagatcga agtcgaagcg gaagtgcggg
     1681 tgatacgtgt gctggtcgtg gacgccgggg ccgtagatcg tgagccacgg gcagagccgg
     1741 ccgtcgtcgt ggaagcgcca cagctgcgtg tactggtacg ggccgacggc gaagttcgcc
     1801 gacagctcga tgccgccgcg gaacgactgc acgcgcaccg gaccttgcag cgagcgcgta
     1861 ccgagtggca cccaccacgg gccacgcgtc tcggcctcgg cctcgacttc gagcgatgcg
     1921 cgctgatgat cgacggtgac gtagggcagc gagccctccc acagcacgcg gcgacctttg
     1981 tagtcggcga ggtacaccat gatgccggcc gactcggtca gccgccaatg gaacttccag
     2041 ttcgcttcat tgatgacgtt gctcatcacc agagctccgc cgggaccacc tgggtcaccg
     2101 tttgatcgac gagatcgacg acagcgatca gcgacgcgtg accgccgctg cgcccgaaga
     2161 tgacggctgc gctgcgccgt gcgaacgcga gatcgttcgg cgtgcgtccc cagtaatgca
     2221 tcgtcgattg cggcgcgtcg cccatctgca gcttgtcgcg cacgcggcca tcgaccattg
     2281 cgatcgagat cgcgagtgct tcctcttcgc gggacagcat cggctgcgcg cgcgtgatgt
     2341 cgagatgcgc gacggtaccg gcgtcgagat cgaccgtcgc gtcgaccgag cgatcggccg
     2401 cgtagtcata ggcgacgacg cgtgcgaggc gatggccggc catccagtgc ggcggcttga
     2461 cgacgagtgg ctcggtgcag atcacgcggt aacgcccaac cttcgccatc gacgacaggc
     2521 ggcgatcttc gatcagcgtg cgcaacgcat cggcggtctc gccgggcgtc agcggatcga
     2581 acagcgtcgg atccgcctcg gtgatgtcgg ccgccggctt gcgcttgccc ttgccgttcg
     2641 gcgccttggc ttcggccttc ggtttggcct ttcctttggc ttctttcgcc gcgggcttcc
     2701 ccgcgggctg cttcgccttt acagcggttt tcgacggagc catggcgcgg actatacacg
     2761 cctacatacg caaatctctg aaaacccaca ggtgttttgt ctattccaag attagatcgc
     2821 gcgtagttgt cgggcgtttt gaacgggcat gaggatgctc cttgcttcgt tgctgttcac
     2881 cgcagcgtgc tcgtcggcat acgccgatca cgccgctgtg caagttggcc cgcgcatcgc
     2941 gctcgccgta tcgcctgaca aggtccgcgc gccgtacgac gtgcaggtca tcaccgagtc
     3001 gggcgagact gcgccgacgt acgcgttcaa gggccgctac tacgtgcaag gcaacgacag
     3061 cgagcactac acgatccgcg tcacgaaccc gaccggcaat cgcgtcgaag cggtggtctc
     3121 ggtcgacggt ctcgatgtcg tcgatggtga agacggtgac ctcaacaagc gcggctacat
     3181 cgtgcccgcg tacggcgagg ttcgcatcga aggtttccgc acgtcgctcg cggacgtcgc
     3241 gtcgttccgg ttctcgtcgg tcgacaactc gtacgccggc aagaagggca agtcgcgcaa
     3301 cgtcggcgtg atcgccgtcg cgatcttcga agagcacgcg ccgcctcccg agcaacagat
     3361 catcgttccc gaagtgacgc gtcgcccgac gtatcactgg acggacgaca tggatggcga
     3421 agcatcggtc gacgccggcc ccgcgccgcg cgatcgcggc tcgagcactg gtggccgcgt
     3481 cgcgcagcac aacaagcaag ccgagaagaa gactgccgct gcggaccgcg cagaagcgaa
     3541 gccgtcgcgt cccgctccgc cgcccccggc tgcaccgggc ggtgttgttg gtggtggtgg
     3601 tcgtggcgcg ccggcgacgg cgaccgcgcc ggctcctcgg cggcagatgg aagaagactt
     3661 cggcggtgac gaaggctact accagcccga cccgactccg gcgccgcgca cgaatcgccc
     3721 gggcctcggc accgagttcg gcgagtcgcg ctactcggcc gcgagctaca cgaagtttgt
     3781 ccgcgctgcg aatcgcccga tcgcgatcgc cgagctccgc tacaacgacg tgaccggcct
     3841 cgtcgcactc ggcattccgg ttcagcccgc gcccgacgag ggcgagatca tgctgcgcga
     3901 gaccgcggac ccgttccccg gtgacaaccg gttctcgcgc ccgcccggcc gctgaaccac
     3961 gccgcatcct ccacagcgcc gcccacgagg cggcgttgtt gtttgtgcgc ccagcatggg
     4021 catccatcca gaggtgaaag tcctctcgtg aacatgttgt tttcggacag ggtcagacat
     4081 gactttcatg caatgcatac aagacatgtc tgaccccgtc cgggctacgg gcccgcgttc
     4141 ggatcttctt cgcagtcgtc gtacgggttc ttgaggcccg gcacgcagaa gcgatcgcgc
     4201 gtcgccgtgc acatcaccgc ggtgactttg ccgtcgaaca cgacatcgac cgagcccttc
     4261 tggcgatcgg tcttgcactc gatgtggcgc ttcgcgccgt agggctcggt gatcttcacg
     4321 ccgctcgggc cgcggaagtt cggcgcgatg tagacgttcg cttcgccggg tcgcgtcaga
     4381 acttcgatcg tgacgttgcc cttcggcggg cgcaccacgc tgtggacgcc ggcatcgcgc
     4441 ggcaccgtcg cgatcacgtg cgtgccagcg tcgggcggca cgtcgacgat caccggcgcg
     4501 tcggccggca cttcgaccgg tgcgtcgcga agctcgacga cgcgcgcggc atcgtgcgga
     4561 gcttcggcat gctcgtcgtc gtgtgattgc agcttgctga tcacgagcac catgccgccc
     4621 gcaccgacga gcagcatgag caggccggcg agccacagcg gccagcgccg cttctcgggc
     4681 accggcacgt cgtcgtagac gtgcgagaac gacaccggtg cgccgggcgc aacgaactcg
     4741 ggctcgtgca gcggccgcgt cgccggcttg ttgcgacgga gcgtctcggc ctgcgtcgag
     4801 cccggtggca gcgctggcaa cgacgagagc tgctcgagct tgggcgcgat gtcctcgagc
     4861 gcggcgagca gcgtgctcat cttgtcgtag cggttctcgc gctctttctc gagcgccttc
     4921 atcacgatcg cttcgagcgc gggcgggatg cgcacctcgg gattcgcttt gctcggcgga
     4981 atgatcggct cgagcatctg catcgcgatc gtgcggatca tgctctcgcc cttgtgcggc
     5041 actttgccga cgagcatctc gtacatgatc acgccaagtg cgtagatgtc gacgcgatgg
     5101 tcggtgtcgc cgcgtcccga tgcttgctcg ggcgccatgt actcgggcgt gccgaacacc
     5161 gagccaacac gcgtgaggcg cggcgcggtc ggatcgggat tgccgtcgac gggctgcacc
     5221 ttcgcgatgc cgaagtcgac gatcttcacg aagtcgggtc ggaagtcgcg atcgatgagg
     5281 aacacgttct cgggtttgag atcgcgatgg acgatgccct tcgcgtgcgc ggcggcgagc
     5341 gcgcgagcga tctgcgatgc aacgcggacg acgcgctcgg cgggcagtgg cgccgcgctc
     5401 ttgatcacct tcgacagcgt cgtgccgtcg acgtactcca tcaccgagta cgtcatgcct
     5461 tcggatgtct tgccgaagtc ggtgacgtcg acgatgttcg gatggccgat ccgcgatgcg
     5521 gccttcgctt cctggatgaa gcgctggatc accgcggcgt cgcgcatgac ctcgcgcttg
     5581 agcactttga tcgcgagtgg ccgctcgatt accgcgtgct tgacggcgaa cacgacgccc
     5641 atgccgcctt cgccgatctt gcgctgcacg tagtagcggc cgtcgagcgt ctcgccgatc
     5701 agcttgtcgt attcgctgac ccgcttgcgg atcgtctcga gcgcttcggc gtgaccttcg
     5761 tgcgccgaca gcgccttcag cttgtttggc gtgttgtcgc cgaggccggg cttggtctgc
     5821 agcttgtcga cgtgcgggct ctcgacgatc ggatcggtat ccggtgcctc ggtggggctc
     5881 tgcgcggcgt gctgcgccga cagcacggtc tgctgttcgg cggcactgac ctggacgagc
     5941 ggtttaccgt cgaacggaca gaagcccggc tctgcgaacg agcgctggca aaccgggcag
     6001 cgaaagctca tctacggtaa cgcatcgtac agcgcagtct tgtagtaggc gaagacttcg
     6061 ctcttggccg cgatgtcgag gatgtctttg tcg
//
LOCUS       DASUFX010000067         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_705828,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000067 DASUFX010000000
VERSION     DASUFX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705828"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFV99_02450"
     CDS             <1..254
                     /locus_tag="VFV99_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058187.1"
                     /translation="HTCVYRWLWRRMRYAGRVAPGLPAGCRQELLPGARFREILRYVL
                     RFRRKQRPRIVDRLERVDGGQRVIALRTPREVDDFLAAL"
     gene            262..780
                     /locus_tag="VFV99_02455"
     CDS             262..780
                     /locus_tag="VFV99_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058188.1"
                     /translation="MQRIAVIGCCGAGKTQLALALGEKLGLPVTHLDQLGWSAGWVEV
                     DNDELARRQQAVFTRDGRWIADGNYGSTMMVRFAVADTIVFMDFATPICLYRVLKRRV
                     RYVGRTRPDMAPGCPESFLDGEFPKFLHYVATFRAKHRPRILAKLAQLDRSQRVITLR
                     SPREARRFLTAL"
     gene            complement(784..1437)
                     /locus_tag="VFV99_02460"
     CDS             complement(784..1437)
                     /locus_tag="VFV99_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX5058189.1"
                     /translation="MRILLALALIAACNKSEKAKPKDPQPHEEMAAKAEPVELPAAGT
                     APTPVTVSKELDPIWTAAIKTLRGKPLQLGEYKGKALLLVNVASQCGNTPQYAALEAL
                     QKKYEAKGFTVVGFPCNQFGGQEPGTAQEIEQFCSANYGITFPITEKIEVNGPSRHEI
                     YKSLETIKDAGGHDGDIRWNFEKFVVSADGTKITRFDPKTKPNDPAVIAAVEAALPH"
     gene            1514..2191
                     /locus_tag="VFV99_02465"
     CDS             1514..2191
                     /locus_tag="VFV99_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058190.1"
                     /translation="MVRLAVVWSVVLAACTFEHGIPVPGGSGNNPDAPVVSSPDGPVL
                     TPVDAPTVLPVDATPVDAPCGDADSDGVCDNVDDWPCGAKPTDPGTMMTMTGSNGATS
                     IRLTQVNLAQMGRYAVATSQQNISIAFSYDIKDTSCAGNCVDQIEIGWVPGGRSGCPF
                     DNAVSKQNGASGNISTMIRTPTNKGSYDLRANIGQNYSCTYNGASGWWGGTTPSASRT
                     IARLCVH"
     gene            2281..>2757
                     /locus_tag="VFV99_02470"
     CDS             2281..>2757
                     /locus_tag="VFV99_02470"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058191.1"
                     /translation="MRHVYADFIDRVAKPARYLGGEYQAVLKADADVDARVCLAFPDV
                     YDIGMSHLGTKIIYSLLNKQPRIACERAFAPWLDMEAELRARNLPLVSLESQRPLREF
                     DVLGISLQYELTFTNMLTILDLGGLPLRAADRAEDATLVLVGGPTASHPEPVAPFMD"
BASE COUNT          436 a          887 c          915 g          519 t
ORIGIN      
        1 cgcacacgtg cgtgtaccgc tggctgtggc gacgcatgcg ctatgccggc cgcgtcgcgc
       61 cggggcttcc agccggctgt cggcaagagc tgctccccgg cgcacgcttc cgcgagatcc
      121 tccgctacgt gctgcggttt cgtcgcaaac agcgtccgcg gatcgtcgat cggcttgagc
      181 gagtcgatgg cggacagcgc gtgatcgcgc tccgtacgcc acgtgaggtc gacgacttcc
      241 tcgcggcgct atgatgtcgg catgcagcgc atcgcggtga tcggctgctg cggcgcgggc
      301 aagacgcagc tcgcgctcgc gcttggcgag aagctcggat tgcctgtgac ccatctcgat
      361 cagctcgggt ggagcgcggg ctgggtcgag gtcgacaacg acgagctcgc gcgtcgccag
      421 caggccgtgt tcacgcgcga cgggcgctgg atcgccgacg gcaactacgg cagcacgatg
      481 atggtgcgct tcgcggtcgc cgacacgatc gtgttcatgg acttcgcgac gccgatctgt
      541 ctgtatcgcg tgctcaagcg ccgcgtgcgg tacgtcggcc gcacgcgtcc ggacatggcg
      601 ccgggatgcc cggagagctt tctcgacggc gaattcccga agttcctgca ctacgtcgcg
      661 acgtttcgcg cgaagcatcg cccgcgcatc ttggccaagc tggcccagct cgatcgctcg
      721 cagcgcgtga tcacgctgcg cagtccgcgc gaagcgcgtc gcttcctgac ggcgctctga
      781 aagctagtgg ggcagcgcgg cctcgaccgc agcgatcact gccgggtcgt tcggtttcgt
      841 tttcggatcg aagcgcgtga tcttcgtgcc gtcggcggag acgacgaact tttcgaagtt
      901 ccagcggatg tcgccatcgt ggccgcccgc gtccttgatc gtctcgagcg acttgtagat
      961 ctcgtggcgg ctgggtccgt tcacttcgat cttctcggtg atcgggaacg tgatgccgta
     1021 gttcgccgag cagaactgct cgatctcctg cgcggtgcct ggctcctggc cgccgaactg
     1081 gttgcacggg aagccgacga ccgtgaagcc cttcgcttcg tacttcttct gcagcgcctc
     1141 gagcgcggcg tactgcggcg tgtttccgca ctgcgaagcc acgttgacga gcagtagcgc
     1201 cttgcccttg tactcgccga gctggagcgg tttgccgcgc agcgtcttga tggcggcggt
     1261 ccagatcgga tcgagctcct tgctgacagt gaccggtgta ggtgctgtac ctgcagccgg
     1321 tagctcgaca ggttcggctt tggcggccat ttcttcgtgc ggttgtggat ctttaggctt
     1381 cgccttttcg gacttgttgc acgcagcgat cagggccaaa gcgaggagga tccgcattgc
     1441 cggtagggta actgagtggc gaagagagca aaagacagac gtatgcgccc ccacctctgt
     1501 tacgttggaa atcgtggtgc gccttgcggt tgtttggtca gtggtgctcg ccgcttgcac
     1561 gttcgagcac ggcattccgg tgccgggcgg aagcggcaat aaccccgacg ctcccgtcgt
     1621 gagctcgccc gacgggccgg tcctcactcc cgtcgacgcg ccgaccgtac taccggtcga
     1681 cgccacgccc gtcgacgctc cgtgcggcga tgccgacagc gatggcgtct gcgacaacgt
     1741 cgatgactgg ccgtgcggcg cgaagccgac cgacccgggc acgatgatga cgatgaccgg
     1801 cagcaacggc gcgacgagca tcaggctgac gcaggtcaac ctcgcgcaaa tggggcgcta
     1861 cgcggtcgcg acgtcacagc aaaacatctc gatcgcgttc tcgtacgaca tcaaggatac
     1921 gtcgtgcgcc ggtaactgcg tcgaccagat cgagatcggc tgggtgccgg gcggtcgttc
     1981 gggctgcccg ttcgacaatg cggtgtcgaa gcagaacgga gcgtcgggca acatctcgac
     2041 gatgatccgc acgccgacga acaagggcag ctacgacctg cgcgcgaaca tcggccagaa
     2101 ctattcgtgc acgtacaacg gagcgagcgg ctggtggggc ggaacgacac cgagcgcgag
     2161 ccgcacgatc gcccggctct gcgtccacta agtgagctgc gccagctcca acagcggcgc
     2221 cgccttatga atgtttgcgt gcgtttggcc ggtttctgag gtagcaacgc aggtcccgcc
     2281 atgcgccacg tctatgccga tttcatcgac cgggtcgcca agcccgctcg gtacttgggc
     2341 ggcgagtacc aggcggtgct caaagccgac gctgacgtcg acgcgcgcgt gtgcctcgcg
     2401 ttccccgatg tctacgacat cggcatgtcc catctcggga ccaagatcat ctactcgctg
     2461 ctcaacaagc agccgcgcat tgcgtgcgag cgcgcgttcg cgccatggct cgacatggaa
     2521 gcggagctgc gtgcgcgcaa cctgccgctc gtatcgctcg agagtcagcg gccgctgcgc
     2581 gagttcgatg ttctcggcat ctcgctgcag tacgagctga cgtttacgaa catgctcacg
     2641 atcctcgacc tcggcgggtt gccgctgcgc gcggcggatc gtgcggagga cgcgacgctc
     2701 gtcctcgtcg gtggcccgac cgcctcgcat ccggagccgg ttgcgccgtt catggac
//
LOCUS       DASUFX010000068        14755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1400602, whole genome shotgun sequence.
ACCESSION   DASUFX010000068 DASUFX010000000
VERSION     DASUFX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400602"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VFV99_02475"
     CDS             complement(<1..1564)
                     /locus_tag="VFV99_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5058192.1"
                     /translation="MKVTIKPMTPGVPRAARLRAYLASAIVTLGLAGAAMKAWALQVD
                     NGDHYRELAKRQHELRQEIPAPRGEVTDTNGNPLAISADADSIWANPREVHDVTGTAE
                     KLAALIHGDAATLEAKLAGDRGFVWIDRHVTPAVAKAVRDAKAKDKGKGLPGIEVVSE
                     PRRWYPAKSVAGPVIGRADIDGNGLDGIELSMNDLLKGKRGAVSALRDARGHKMLGDE
                     LAPAQPGAHVRLTLDRSIQAITDQALAEAVIAKNAKNGAAVVLDVATGKVLALSSYPT
                     FDPNGEYVNGARNRPVTDAYEAGSVMKVFTIAAALEDGTVKPSTGFVTGPGFRVPGRP
                     RPITDVHAQPYLTVSEIIKVSSNIGAAKIALRLGSEKLHDYLKKFGFGAQSGIELPGE
                     QTGKLRPGSTWRDSELATMAFGYGLTVTPLQIAAGLAAIGNRGVYHAPRIVEEVIGDD
                     GQVIYRGNGEAKQMISAKTAEQMRVMLASVFEKAHGPGTLGGTAKDINVPGFRCGGKT
                     GTAHKYDPAIKTY"
     gene            complement(1561..1833)
                     /locus_tag="VFV99_02480"
     CDS             complement(1561..1833)
                     /locus_tag="VFV99_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5058193.1"
                     /translation="MVLAATMLTALGVVRVNRQHDVLRLGYQLNRETELVRQQREVQR
                     RLEVEIATLTAPERIRRLATQLGMTTVAPDKIRIVDVPNRKKVAQQ"
     gene            2002..2448
                     /locus_tag="VFV99_02485"
     CDS             2002..2448
                     /locus_tag="VFV99_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058194.1"
                     /translation="MRAVAVVVLLACGCTQTQARKAHRAGELATMGGLIGVLACGVTA
                     ALVPSHEDTIMAVGLGFVPISILGALVYISTDERANEDHGPTLTLHERNRAAAWELTK
                     QAETAARGRDCTQVQAIDPRVRDLDADFHLNVFMRDIAIQTCLREH"
     gene            2472..3014
                     /locus_tag="VFV99_02490"
     CDS             2472..3014
                     /locus_tag="VFV99_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058195.1"
                     /translation="MRLTIGVAVRLASVRRLKHLVLAACLLAGCAGTPKKNERRAELA
                     IGASLLGVIASSVAMAAVPQGKPVLIPLTITFGGLAVASAIVYGVEHAKVADEPSGPP
                     PKPNPAWEYTKKAEAAARAGRCDEVRDLDATVLMLDADFHDVVFARDVAIKHCMEAAP
                     AAKAKPTSKATPPPKSKHKR"
     gene            complement(3149..4126)
                     /gene="rsmH"
                     /locus_tag="VFV99_02495"
     CDS             complement(3149..4126)
                     /gene="rsmH"
                     /locus_tag="VFV99_02495"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5058196.1"
                     /translation="MPDFVHESVLVGEVLEHLSPREDGVYVDCTVGGGGHSSAIPGRL
                     IGIDRDPAALAAAAIRLGVGTETNPGTERERITLVHGELGNIRKILADLGVDKVDGFL
                     ADLGVSSPQLDHADRGFSFAKEGPLDMRMDPTRGQTALDLLRSIDVDALGTVIGELGE
                     ERYAKRIARLIKEAIREDRIETTTDLAKVVAQGIPVVEQRKSKIHPATRTFQALRIAV
                     NAELDQLTQFLAAFPDLLKPGGRCVIISFHSLEDRLVKNAFRDLTWTSSLPARLAADA
                     GERVAPVVEVLTRKAVFATEAEVERNPRARSARLRACMRLDTPNVPARR"
     gene            4282..5088
                     /locus_tag="VFV99_02500"
     CDS             4282..5088
                     /locus_tag="VFV99_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5058197.1"
                     /translation="MLRYKETEDIDARFSEVERTVTAWRQAITTANQVARQEFNNRLR
                     ISLIHHDAALEGEVLSYSEIKAAVDPSIISDPSLIPSYEEIRRFDDACNYAAESALKK
                     KPFKLDTVREIYSILAPDEKAKGLPFRKENPLHRLYYHDIAPPEKIVPAMRKLSEWID
                     DPAQKHTHVIEKVASLHSKFMAIFPWAKESGRTIRIASNLLLQEAGYPIAIIHSIDRQ
                     RYYEALRGDHIGLTSLYLEAVQTTAETELRVYEEAGKAPAKKRTKKSSTG"
     gene            5102..5521
                     /gene="arfB"
                     /locus_tag="VFV99_02505"
     CDS             5102..5521
                     /gene="arfB"
                     /locus_tag="VFV99_02505"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5058198.1"
                     /translation="MHDVVVTPRLTIPAGELSLAFARSGGPGGQNVNKVSSKVELRWN
                     PTTSAALTLDDRSWLLSRLANRLTNDGTLIVTSTATRDQLKNRDDAFSKLTLIVRTAL
                     ERPKARRATKPSRASKRRRVDDKRHRAQIKRDRRGDD"
     gene            5562..6593
                     /locus_tag="VFV99_02510"
     CDS             5562..6593
                     /locus_tag="VFV99_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0007275 - multicellular organism
                     development [Evidence IEA];
                     GO_process: GO:0030178 - negative regulation of Wnt
                     signaling pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dickkopf N-terminal cysteine-rich
                     domain-containing protein"
                     /protein_id="HEX5058199.1"
                     /translation="MRIVLALAMVVMATGCPDDDGDGSNYVAINQIKSAYQDAYCQYL
                     VRCGQFPDQAACVGANLSSSFSLDPNIVASVLAGKTIYNGGAVKACFAALAQRSCDKT
                     SASARSVPLACRDFLRGTLGAGQACLLDEECVSQQCVGSQPDISCAMGSCLGDTPPAI
                     QLAAIGEQCTTTEGCVEGAYCDTSLLRCAALLDSGMPCTNNGECNYGLGCGGATGART
                     CKLLPTEGEPCPDFLCREDGQYCNPNGVCAKVGLPPATCTSNAECSLYYPCDFTTSTG
                     MCKQGPGLDQSCSGTNRCFAVGTFCDSSTFMCVAVRPIGGACSNDTQCESEFCDFDAG
                     MCAAPQTCF"
     gene            complement(6597..7148)
                     /locus_tag="VFV99_02515"
     CDS             complement(6597..7148)
                     /locus_tag="VFV99_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058200.1"
                     /translation="MVLVVPPATPSRATGEIEKLLVELGHPQLADLDDEQLVLAEQHD
                     DLFEEIAAFARRGWAYRFDAETGMDYTPREYELVLLEILRPLGVSDVELDDKTCILHV
                     DARKIHVQVPEQDDDWLDVGWIFEVANLAVSETGWLLLGIDDGDMHGVIIGVVPHDVW
                     SAISDKQLAGAWRAMTPVETLFN"
     gene            complement(7151..8608)
                     /gene="ahcY"
                     /locus_tag="VFV99_02520"
     CDS             complement(7151..8608)
                     /gene="ahcY"
                     /locus_tag="VFV99_02520"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5058201.1"
                     /translation="MDTRTEFPAFKIKDLTLAELGRKKIRMAEKEMPGLMSLRAEYGA
                     QKPLKGARVAGCLHMTIETAVLIETLVALGAEVTWTSCNIYSTQDEAAAAIAKAGIPV
                     FAWKGETLEDYWQNIELQLTAFQGGKGPNLILDDGGDLTLLVHKGAEVEKKGSAPNPA
                     TATNEEMRVIFNVLNASLAKSKDRFTKMAAEIKGVSEETTTGVHRLYDMAKSGTLLFP
                     CINVNDSVTKSKFDNLYGCRESLFDGVKRATDVMVAGKVVVVAGYGDVGKGCAHAARG
                     LGAKVLITEIDPICALQAAMEGYDVVTMEEAAPIGDIFVTATGCMDVIRSEHFTAMKD
                     EAILANIGHFDSEIQVAWLENNKEIKEENIKPQVDQFIFPNGKRITLLARGRLVNLGC
                     ATGHPSFVMSNSFSNQTIAQMALWANATKGKDDMTGMKFEAGKVYVLPKKLDEKVARL
                     HLAKVGVKLTKLSQEQAEYLGVPVDGPFKPDHYRY"
     gene            complement(8592..9542)
                     /locus_tag="VFV99_02525"
     CDS             complement(8592..9542)
                     /locus_tag="VFV99_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5058202.1"
                     /translation="MQAGRADLFRLLGDEDRLRLLALCAEEELTVGELAQLLGESQPQ
                     ITKKSQPLREVGLLNARRDGTRTLLKVDAEPDPVIDAAIQEGRTLCSRDGSLAKIARI
                     VSQREDLSRRLFETPTEPAPAPPADASLLAWTPLLAPLLPGRALALDIGTGEGALLPL
                     LSPLYERVIAVDRSPARLARCGARVADWGLPNVRLREGNIDDASLAEEVQSRGGADLV
                     VMARMLVHASRPQDAIASATRLLRAGGHLAIVDYVPHDDESLREQGHVWLGFEPAKLR
                     GWLEAAKLAPVALAPFPSAHQPPLQLAVGKKHGDHHGYAH"
     gene            9764..11467
                     /locus_tag="VFV99_02530"
     CDS             9764..11467
                     /locus_tag="VFV99_02530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058203.1"
                     /translation="MSTGSGDKPGGGSSPGRPAADTPGRLVVRSLDGTTDTSMGAGDT
                     AVAVAKDSSEHAMLETIGARPTPLAGTPALSSTGEIVGSTLSGRYLVTRKVGQGGMGA
                     VYEATHTLIGKRVAVKVLLEKYAQREAIVKRLKQEAQLASSIGNEHIIDITDFGNTED
                     GRTFVVMEFLEGESMAECLSRESKLPENRILRVAQQTASALAAAHAKGVVHRDIKPEN
                     IFLLKRKEMDFVKVVDFGISKSLRQSSEEEEAVRLTQTGMVLGTPLYMSPEQARGDED
                     LDHRVDIYALGVIMYEAATGRVPFSGNNYLSVISQVLNEEPKPLREIRPELSEEFEAI
                     VLRAMAKERNDRYANATDMLHDLGALMEDPTHSTERAKITGPRRLMPRAPKIPRMAFA
                     IGGIGIAVGAAAIIVTLLMGSKQKKANGQHLPAAGGDAGVAAIATLDGGPEQPAVETI
                     KLRIVTDPPGAEVYRESELIGNAPTEIPFVRNNKEVTITAQMAGYDDAKIVVNPLERQ
                     NGQELKLKLKKPVKGPPKTTKIKPAGQGTNQGSGSGPANHTGGELGTNPFATGGTVQK
                     K"
     gene            11464..13158
                     /locus_tag="VFV99_02535"
     CDS             11464..13158
                     /locus_tag="VFV99_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058204.1"
                     /translation="MRVSFAVFVIVMSATAAHAQVGDPRTRDAGYIGKEIPILEIDDC
                     PPPPQVTPEDLRKIGSEHFERGETLYVQGDYKGAVHELVAAYCISPYYTILKDIGQAY
                     ERELDYEKAIAYLERFVVAVPKDAQRANSCAPDPQDEKKNVIARINVLGALKAKILIN
                     TDPADSKVTLSNDAGIAGRGRSGQELEVAGGRYEVLIEHDGYHSVTQEIPAKIGKPYT
                     IFAKLEPLKGRLRVRVVPADARLFLDSKQVGAGAYEGELEGKRYTLSAEAPGRISVSR
                     EIEVFPNRDTPISFELPPEPQVGRRQLLGYAGIGGAFAGGFLGAATTNGGLIAGGVGA
                     GAAAGVLGAYFGTPKDVPLGTSSLTITSSLIGGTAVGAGALLATDDPQKVLPAIGGGL
                     IAGAGIGYYAGSRLRIRPGDAAVINSGALWGGVTGGLFVGSFDAGRTVGSGIVLSGLG
                     MGTLGGVLMTRYFNVSRGRAALIDVGGTVGIFIGIAIENVVTQANCDASDPSCNQSER
                     ERTANYSLGGMAAGLILAGVLTRNMDAPKLSVSPVISKTSTGAGPSTSTFGFGGRF"
     gene            13176..>14755
                     /locus_tag="VFV99_02540"
     CDS             13176..>14755
                     /locus_tag="VFV99_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5058205.1"
                     /translation="MEHRRSACPLDCPDLCGLTVTVDNGRVVEVDGDHRAPLTNGFIC
                     GKVRKIAEHVHGDARVLHPMIRTGAKGSGQWRQVSWDEALDTIAERIQTIRARAGSEA
                     ILPYHYGGSNGWLTEGALATRFFRRLGASNLDRTFCAGATTAATHGLYGKMAGIALED
                     YEHSKLIVLWGVNPSATGIHLVPVIERAIANGAKLVVVDPRRTPLARRADLHLPVRPG
                     ADLPVALAIVNQLFARGHADLAFLDKHAVDVAELRERAGAWSLEAAAREANVDVAALD
                     RFVELYASASPAGIRLGYGLERNRNGGSAVAAILAMPAVAGKFGVRGGGFTMSNGDAK
                     WTVSAEIAIDTPAPPTRHINMSEIGRVLATVNDPRIELLFIYNCNPVSTAPDQRTLVE
                     QLAREDRFVVVHEQVWTDTCNLADVVLPATTFLEHRELRRGYGNMRMYDSPAVIDPVG
                     EARSNNQLFGALLRKLDLVRPGDAMTDDELVAKTFAAGDGAALQAQLAEKHVAQIPGN
                     ARPVMFVDEFPSTPDRKI"
BASE COUNT         2564 a         4815 c         4824 g         2552 t
ORIGIN      
        1 agtacgtctt gattgcagga tcgtacttgt gcgcggtgcc ggtcttgccg ccacagcgaa
       61 atccggggac gttgatatcc ttcgcagtac cgccgagcgt gcctgggccg tgcgctttct
      121 cgaacaccga ggcgagcatc acgcgcatct gctcggcggt cttcgcagag atcatctgct
      181 tcgcttcacc gttgccgcga tagatgacct ggccgtcgtc gccgatgact tcctcgacga
      241 tgcgcggtgc gtgatacacg ccgcggttac cgatcgcggc gagaccggcg gcgatttgca
      301 gcggcgtgac cgtgagaccg tagccgaatg ccatcgtcgc gagctcggag tcgcgccacg
      361 tcgagcccgg ccgcagcttg ccggtctgct cgccgggtag ctcgatgccg ctctgcgcgc
      421 cgaagccgaa cttcttgagg tagtcgtgca gcttttcact gccgaggcgc agcgcgatct
      481 tcgcggcacc aatgttcgac gacaccttga tgatctcgct gaccgtcagg tacggctggg
      541 cgtgaacgtc ggtgatcggg cgcgggcggc cggggacgcg gaagcctggg ccggtgacga
      601 acccggtgct cggtttgacg gtgccgtcct cgagcgcggc ggcgatcgtg aacaccttca
      661 tcaccgagcc ggcctcgtac gcgtcggtga ccggccggtt gcgcgcgccg ttcacgtatt
      721 cgccgttcgg atcgaacgtc ggatagctcg acagtgcgag taccttgccc gtcgcgacat
      781 cgaggacgac ggcggcgccg ttcttcgcgt tcttcgcgat cacggcctcg gcaagcgcct
      841 ggtctgtgat cgcctgaatg ctgcggtcga gcgtgagccg cacgtgggca ccgggctgcg
      901 ccggcgcgag ctcgtcgccg agcatcttgt ggccgcgtgc gtcgcgcagt gcgctcaccg
      961 caccgcgctt gcctttgagc agatcgttca tcgacagctc gatgccgtcg agcccgttgc
     1021 cgtcgatgtc ggcacggccg atcacgggac cggcgaccga cttcgcggga taccagcggc
     1081 gtggctcgct cacgacctcg atgccgggaa ggcctttgcc cttgtccttg gccttcgcgt
     1141 cgcgcacggc ctttgcgacc gcgggcgtga cgtgacgatc gatccacacg aagccgcgat
     1201 cgcctgcgag cttcgcttcg agcgtcgccg cgtcgccgtg aatgagtgcg gcgagcttct
     1261 cggcagtacc ggtgacgtcg tgcacctcac gcggattcgc ccagatcgaa tcggcatcgg
     1321 cgctgatcgc gagcggattg ccgttcgtgt cggtgacctc gccgcgcggc gctgggatct
     1381 cctggcggag ctcgtgctgg cgtttggcga gctcgcgata gtgatcgccg ttgtcgactt
     1441 gcagcgccca cgctttcatc gcggcgccag caagaccgag cgtgacgatc gcactcgcga
     1501 gatacgcgcg tagccgcgcc gcgcgtggca cgcccggcgt catcggcttg atcgtcacct
     1561 tcattgctgc gcgaccttct tgcggttcgg cacgtcgacg atgcgaatct tgtccggcgc
     1621 gaccgttgtc atcccgagct gcgttgcgag gcgacgaatc cgctcgggag ccgtcagcgt
     1681 cgcgatctcg acttcgagtc ggcgctgcac ctcgcgttgt tgccgtacga gctcggtctc
     1741 gcgattgagt tgatacccga gtcgcagtac gtcgtgctgc cggttcacac gtacgacgcc
     1801 gagcgcggtc agcatcgtgg ctgcgagcac catcgcgatc acgatcgatc gaactggcgc
     1861 agcgccttct gcgcgcttga agattcggta aacagaccgg gctgccgacg acgtcatgtc
     1921 acgtcgctcc actacaaccc cgtgcgattg catgggcaat tttttgggcg acactcccga
     1981 cgaaaaccgc cacacttgat catgcgtgcg gtcgctgtcg tcgtgttgct ggcatgtggg
     2041 tgcacgcaga cgcaggcgcg gaaggcgcac cgggccggcg agctcgcgac gatgggcggg
     2101 ctcatcggtg tgctcgcctg cggcgttacc gccgcgcttg tcccctcgca cgaggacacg
     2161 atcatggctg tcgggctggg cttcgtcccg atcagcatcc tcggtgcgct cgtctacatc
     2221 agcaccgacg agcgcgcgaa cgaggatcac ggcccgacgt tgaccctgca cgagcggaac
     2281 cgtgcggcgg cgtgggagct gacgaagcaa gcagagaccg cggcgcgtgg tcgggactgc
     2341 acacaagtac aggcaatcga tccgcgcgtg cgtgatctcg acgccgactt tcacctcaac
     2401 gtgttcatgc gcgacatcgc gatccagacc tgcctgcgcg agcactgatc cacgcgtgat
     2461 acgctgactc gatgcggctg acgatcggcg tggcggtacg gctggccagc gttcgtcgac
     2521 tgaagcacct ggtgctcgcc gcgtgcctcc ttgcgggctg cgcgggtaca ccgaaaaaga
     2581 acgaacgccg cgccgagctc gcgatcggtg catcgctcct cggcgtgatc gcgtcgtcgg
     2641 tagcgatggc ggcagtaccg cagggcaagc cggtcctgat cccgctcacg atcacgttcg
     2701 gcggactcgc ggttgccagc gcgattgtct acggcgtcga gcacgccaag gtcgcagacg
     2761 agccgtcggg tccgccgcca aagccgaacc cggcatggga atacacgaaa aaggcggaag
     2821 ccgcggcgcg tgccggtcgc tgcgacgagg tgcgtgacct cgacgcgacc gtgctgatgc
     2881 tcgacgcaga ttttcacgac gtcgtgttcg cgcgcgacgt cgcgatcaag cactgcatgg
     2941 aagcggctcc agccgcgaag gcgaagccga cgtcgaaggc aacgccgccg ccgaagtcga
     3001 agcacaagcg gtagcgccgc tccgacaggt tcgttttcgt ccggcagccg gccattaccg
     3061 ccgggccggc acgtgcggca tgtcgccccg aacgtaccgg tgtcgccccg aacgtaccgg
     3121 ggttagtttt tcgtccggca gtcggccatt accggcgggc cggtacgttc ggcgtgtcga
     3181 ggcgcatgca ggcacgcagt cgcgccgacc gcgcgcgggg attgcgttcc acctcggcct
     3241 cggtcgcgaa caccgccttg cgggtcagca cctcgaccac gggcgcgacg cgctcgccgg
     3301 cgtcggccgc aagtcgcgcg ggcagcgacg atgtccacgt cagatcacga aacgcgttct
     3361 tcaccaaccg atcctcgagc gaatgaaaac tgatgatcac gcagcggccg cccggcttca
     3421 gcaagtcagg aaacgcggcg aggaactgcg tgagctgatc gagctcggcg ttcaccgcga
     3481 tgcgcagtgc ctgaaacgtg cgcgtcgccg gatgaatctt cgacttgcgc tgctcgacca
     3541 ccggaatccc ctgcgccacc accttcgcca gatccgtcgt ggtctcgatg cggtcctcgc
     3601 ggatcgcctc tttgatcagc cgcgcgatcc gcttcgcata ccgctcttcg ccaagctcgc
     3661 cgatcaccgt accgagcgcg tccacatcga tcgaccgcaa cagatcgagc gcggtctgtc
     3721 cgcgcgtcgg atccatgcgc atgtcgagcg ggccctcctt cgcgaacgag aaaccccggt
     3781 cggcatggtc gagctgcggt gacgacacgc cgaggtcggc aagaaagccg tcgaccttgt
     3841 cgacacccag gtccgcgagt atcttgcgaa tattgccgag ctcgccgtgc acgagcgtga
     3901 tccgctcacg ctccgtaccg gggttagttt ctgtgccgac accaagccgg atcgcagccg
     3961 cggcaagtgc ggcggggtcg cgatcgatgc cgatgagccg tccgggtatc gcgctcgagt
     4021 ggccaccgcc acccaccgtg cagtcgacgt aaaccccatc ttctcgcggg gataggtgct
     4081 cgagcacttc gccaactaga accgactcgt gcacgaagtc cggcacgtgg cgtgagtacc
     4141 acgcagggtg cgcaagactg cagaaatctt gcggttgaat cgcaactgat gtgcagttac
     4201 gtgtaaggaa gtgtcgccct gtttgcttga cgatccggat ggcgtcccgt agactccacc
     4261 ggttcgtggg ataggataac tgtgctcagg tacaaagaaa ccgaggacat tgacgcgcgg
     4321 ttcagcgagg tcgagcgcac cgtcacagcg tggcgacaag cgatcacgac tgcgaaccag
     4381 gtcgcgcgcc aagagttcaa caaccgcctt cgcatctcgc tcatccatca cgatgcagcg
     4441 ctcgaaggcg aggtcctgag ctacagcgaa atcaaagcgg ccgtcgatcc gagcatcatc
     4501 tctgatccgt cgctcattcc gtcgtatgaa gaaattcgcc gcttcgatga cgcgtgtaac
     4561 tacgcggccg aatcggcgct gaagaagaag ccgttcaagc tcgatacggt tcgcgagatc
     4621 tattcgatcc tcgcacccga cgagaaggcg aaaggtctcc cgttccgcaa ggagaacccg
     4681 ctgcaccggc tctactatca cgacatcgcg ccgccggaga aaatcgtccc ggcgatgcgc
     4741 aagctgagcg agtggatcga cgatcccgcg caaaagcaca cgcacgtgat cgagaaggtc
     4801 gcatcgctcc actccaagtt catggcgatc ttcccgtggg cgaaggagag cggtcgcacg
     4861 attcgcattg cctcgaacct cctgctccag gaagcgggct acccgatcgc gatcattcac
     4921 tcgatcgatc gccagcggta ctacgaagca ctgcgcggcg atcacatcgg gctcacctcg
     4981 ctctatctcg aagcggtgca gaccactgcc gagaccgagc tgcgcgttta cgaagaggcc
     5041 ggcaaggccc cggcgaagaa gcgcaccaag aagtccagca cgggctagtc ggcggcgtgt
     5101 cgtgcacgac gttgtcgtta cgccgcggct gacaattccc gcgggcgagc tgtcactggc
     5161 attcgcgcgc tcgggcggac cgggcgggca gaacgtcaac aaagtctcga gcaaagtcga
     5221 gctgcgctgg aacccgacga cgtcggcggc gctcacgctc gacgatcgct cgtggctgct
     5281 ctcgcgcctc gcgaaccggc tcaccaacga cggcacactg atcgtgacgt cgaccgcgac
     5341 acgcgatcag ctcaagaacc gcgacgacgc gttcagcaag ctcacactga tcgtgcgcac
     5401 cgcgctcgag cgaccgaaag ctcgccgcgc gacgaagccg agccgcgcat cgaagcgccg
     5461 ccgtgtcgac gacaagcgac atcgcgcaca gatcaagcgc gaccgccgcg gcgacgacta
     5521 gtcttcgacc ccgtcgaacg gcttcacggt accatcggcg catgcggatc gtgctcgcgc
     5581 tcgcaatggt cgtgatggcg acgggatgtc cagacgacga cggcgacggc agcaactacg
     5641 tcgcgatcaa tcagatcaaa tccgcgtacc aggacgccta ctgccagtat ctcgttcgct
     5701 gtggacagtt cccggatcag gcggcgtgcg tcggtgcgaa cctctcgagc agcttctcgc
     5761 tcgatccgaa catcgtcgcg agcgttctcg ccggcaagac catctacaac ggcggcgcgg
     5821 tcaaggcgtg cttcgctgcg ctcgcgcagc gctcgtgcga taagacgtcg gcgagcgctc
     5881 gcagcgtacc gctcgcgtgc cgtgacttcc tgcgcggaac gctcggcgcc gggcaagcat
     5941 gcctgctcga cgaagagtgc gtctcgcaac agtgcgtcgg gagccagcca gacatctcgt
     6001 gcgcgatggg ctcgtgtctc ggcgatacgc cgccggcgat ccaactggcc gccattgggg
     6061 agcagtgcac gacgacggaa ggttgcgtcg agggcgcgta ctgcgacacg tcgctgcttc
     6121 ggtgtgccgc gctgctcgat tccggcatgc cgtgcacgaa caacggcgag tgcaactacg
     6181 gtcttggctg cggcggagcg accggtgcgc gcacgtgcaa gctattgccg acggagggcg
     6241 agccgtgccc ggacttcttg tgccgcgagg acgggcagta ctgcaacccg aacggcgtct
     6301 gcgcgaaggt cggcctgcct ccggcgactt gcacgtcgaa tgcggagtgc tcgctctact
     6361 acccgtgcga cttcaccacc tcgacgggaa tgtgcaagca aggtccgggg ctcgatcaat
     6421 cgtgctcggg cacgaatcgg tgcttcgccg tgggcacgtt ctgcgactcg tcgacattca
     6481 tgtgcgtcgc ggtgcggccg atcggcggcg cgtgctcgaa cgacacccag tgcgagagcg
     6541 agttctgcga cttcgacgcc ggcatgtgcg cggcgccgca gacgtgcttc tagcgtctag
     6601 ttgaacagcg tctcgaccgg cgtcatcgcg cgccatgcgc cggcaagctg cttgtcactg
     6661 atcgccgacc acacgtcgtg tggcacgacg ccgatgatca cgccgtgcat gtcgccatcg
     6721 tcgatgccga gcagcagcca gccagtttcg gagaccgcga gattcgcaac ctcgaagatc
     6781 cagccgacgt cgagccagtc gtcgtcctgc tccggcacct gaacgtggat cttccgcgca
     6841 tcgacgtgca ggatgcatgt cttgtcgtcg agctcgacat cgctgacgcc gagcggtcgc
     6901 aggatttcaa gcagcacgag ctcgtactcg cgcggcgtgt aatccatgcc cgtctcggcg
     6961 tcgaagcgat atgcccagcc acggcgcgcg aacgccgcga tctcctcgaa caggtcgtcg
     7021 tgttgctcgg cgagcacgag ctgctcgtca tcgagatcgg ccagttgtgg atgaccgagc
     7081 tcgacgagca gcttttcgat ttcgccagtg gcccgcgacg gcgtggccgg agggacgacg
     7141 agcaccacgg ttagtatcga tagtgatccg gcttgaacgg accgtcaacc ggcacgccga
     7201 ggtactccgc ctgctcctgc gacagcttcg tcagcttgac gccgaccttt gccaggtgca
     7261 gacgcgccac cttctcgtcg agcttcttgg gcagcacgta gaccttgccg gcctcgaact
     7321 tcatgccggt catgtcatcc ttacccttcg tcgcgttcgc ccagagcgcc atctgcgcga
     7381 tcgtctggtt cgagaacgag ttcgacatca cgaacgacgg atggccggtc gcgcagccga
     7441 ggttgacgag gcggccgcgg gcgagcagcg tgatgcgctt gccgttcggg aagatgaact
     7501 ggtcgacctg cggcttgatg ttctcttcct tgatctcttt gttgttctcg agccacgcaa
     7561 cctgaatctc gctgtcgaag tgcccgatgt tcgcgaggat cgcttcgtcc ttcatcgcgg
     7621 tgaagtgctc gctgcggatc acgtccatgc agcccgtcgc ggtgacaaag atgtcgccga
     7681 tcggcgcagc ctcttccatc gtgacgacgt cgtagccttc catcgccgcc tgcagcgcgc
     7741 agatcggatc gatctcggtg atgaggacct tcgcgccgag accgcgcgcg gcgtgcgcgc
     7801 agcccttgcc gacgtcaccg tagccggcga cgacgacgac cttgccggca accatcacgt
     7861 ccgtcgcgcg cttgacgccg tcgaacagcg attcgcggca accgtaaagg ttgtcgaact
     7921 tcgacttcgt gaccgagtcg ttgacgttga tgcacgggaa cagcagcgtg ccgctcttcg
     7981 ccatgtcgta gaggcggtgc acgccggtcg tggtctcctc ggagacgccc ttgatctcgg
     8041 cggccatctt cgtgaagcga tccttgctct ttgcgaggct cgcgttcagc acgttgaaga
     8101 tcacgcgcat ctcttcgttc gtcgcggtcg ccgggttcgg agccgagccc ttcttctcga
     8161 cctcggcgcc cttgtggacg aggagcgtca gatcgccacc gtcgtcgagg atgaggttcg
     8221 gacccttgcc accctggaac gcggtgagct ggagctcgat gttctgccag tagtcctcga
     8281 gcgtctcgcc cttccacgcg aacaccggga tgccggcctt cgcgatcgcg gcggcggctt
     8341 cgtcctgcgt cgagtagatg ttgcacgacg tccacgtgac ttcagcgccg agcgcgacga
     8401 gcgtctcgat cagcaccgcg gtctcgatcg tcatgtgcaa gcagcccgcg acgcgcgcgc
     8461 ccttgagcgg cttctgcgcg ccgtactcgg cacggagcga catcaggccg ggcatttcct
     8521 tttcggccat gcggatcttc ttgcggccga gctcggcgag cgtgaggtct ttgatcttga
     8581 aggcggggaa ttcagtgcgc gtatccatga tggtctccgt gtttcttgcc aacagctaat
     8641 tgcagagggg gttgatgggc ggacgggaac ggcgcgagcg cgaccggcgc gagcttggca
     8701 gcttcgagcc atccacgcag cttcgctggc tcgaaaccga gccagacgtg cccttgctcc
     8761 cgaagtgatt cgtcgtcgtg cggaacgtag tcgacgatcg cgagatgacc gccggcgcgc
     8821 agcaggcgcg tggccgatgc gatcgcatct tgcggacgac tcgcatgcac gagcatgcgc
     8881 gccatcacga cgaggtcggc gccaccgcgc gattgcactt cctcggcgag gctcgcatcg
     8941 tcgatgttgc cttcgcgcag tcgcacgttc ggcaggcccc agtcggcgac gcgtgcaccg
     9001 caccgggcga ggcgcgccgg gctgcgatcg accgcgatca cgcgttcgta aagcggggac
     9061 agcagcggga gcagtgcgcc ctcgccagtg ccaatgtcga gcgcgagcgc tcggccaggc
     9121 aacagcggtg cgagcagcgg agtccaagcg agcagcgatg catcggcagg cggcgcaggt
     9181 gcgggctcgg tcggcgtttc gaacaggcgg cgggacagat cctcccgctg cgacacgatg
     9241 cgtgcgatct tcgcgagcga tccgtcgcgg ctgcacagcg tgcggccttc ctggatcgcg
     9301 gcgtcgatga ccgggtcggg ctcggcatcg accttgagca gcgtccgggt gccgtcccgg
     9361 cgggcgttga gcaagccgac ctcgcggagc ggctgcgact ttttcgtgat ctgcggctgg
     9421 ctctcaccga ggagttgcgc caactcaccg accgtcagct cctcctccgc acacagcgca
     9481 agcaggcgca gccggtcctc atccccgagg agacggaaca gatcagcgcg tcctgcctgc
     9541 acatcagtct atatattcca tcattcgaat gttggcaacg atgccgtacc ggggtttgtt
     9601 ttggtctgcc gatctagtcc gccaactaac cccgtagcgg tccgcccgca ggtccgcccg
     9661 cagtcccata tgtgggcaaa gttcaaaatg tttcggcacg taaggacgat gggccggttt
     9721 gacttggctg gggtggaccc ctaccatcgt cggtaaaggg cgaatgtcta cgggttctgg
     9781 cgacaagccc ggtggcggtt catcgccggg tcggcctgcg gccgatacac ccggtcggct
     9841 cgtcgtccgt tcgctcgatg gcaccaccga cacatcgatg ggtgccggtg acactgcggt
     9901 cgcagtcgcg aaagattcga gcgagcacgc gatgctcgag acgatcggcg cgcggcccac
     9961 accgctggcc ggcacgccgg cgctgagctc gaccggcgag atcgtcggct cgacgctgtc
    10021 gggccgctat ctcgtcacgc gcaaggtcgg tcagggcggc atgggcgccg tttacgaagc
    10081 gacgcacacg ctgatcggta agcgcgtcgc cgtaaaagtg ttgctcgaga agtacgcgca
    10141 acgcgaagcg atcgtgaagc gcctcaagca agaggcgcaa ctcgcgtcgt cgatcggcaa
    10201 cgagcacatc atcgacatca ccgacttcgg caacaccgag gacggtcgca cgttcgtcgt
    10261 gatggagttc ctcgagggcg aatcgatggc cgagtgtctc tcgcgcgaat cgaagctccc
    10321 cgagaaccgc atccttcgcg tcgcgcagca gactgcatcc gcgctcgccg ccgcgcacgc
    10381 gaagggcgtc gtccaccgcg acatcaagcc ggagaacatc ttcctgctca agcgcaagga
    10441 gatggacttc gtgaaggtcg tggacttcgg catctcgaag tcgctgcggc aatcgagcga
    10501 ggaagaagaa gccgttcgcc tcacgcaaac cggcatggtg ctcggtacgc cgctgtacat
    10561 gtcgcccgag caggcgcgcg gcgacgagga tctcgatcac cgcgtcgaca tctacgcgct
    10621 cggcgtcatc atgtacgaag ccgccaccgg gcgcgtgccg ttctccggca acaactatct
    10681 ctcggtgatc tcgcaagtcc tcaacgagga accgaagccg ctgcgcgaga tccgccccga
    10741 gctcagcgag gagttcgagg caatcgtcct gcgcgcgatg gcgaaagagc gcaacgatcg
    10801 ctacgcgaac gcgaccgaca tgctgcatga cctcggcgcg ctcatggagg atccgacgca
    10861 ctcgaccgag cgcgcgaaga tcaccgggcc acgccggctc atgccgcgcg cgccgaagat
    10921 tccccgcatg gcgtttgcga tcggtggcat cggcatcgcc gtcggtgccg ccgcgatcat
    10981 cgtgacgctg ctgatgggca gcaagcagaa gaaggcgaac ggccagcatc tgcccgccgc
    11041 cggtggcgat gctggcgtag ccgcgatcgc gactctcgat ggcggcccgg agcagccggc
    11101 cgtcgagacg atcaagctcc gcatcgtcac ggatccgcca ggcgccgagg tctatcgcga
    11161 gtccgagctg atcggaaatg ctccgaccga gatcccgttc gtcaggaaca acaaggaagt
    11221 cacgattacg gcgcagatgg ccggctacga cgacgcgaag atcgtcgtga atccgctcga
    11281 gcgccagaac ggccaggagc tgaagctcaa gctcaagaag ccggtgaagg gcccgccgaa
    11341 gacgacgaag atcaagccag cggggcaggg cactaaccag ggcagcggca gtggcccggc
    11401 gaaccacacg ggtggcgagc tgggtaccaa tccgttcgcg actggcggta cagttcagaa
    11461 gaagtgaggg tctcgttcgc ggtcttcgtg atcgtgatgt cagcgactgc ggcacacgcg
    11521 caggtcggtg acccgcgtac gcgcgatgcc ggctacatcg gcaaagagat tccgatcctc
    11581 gagatcgacg actgcccgcc gccaccgcaa gtcacgcccg aggatctgcg caagatcggc
    11641 tcggagcact tcgagcgcgg cgagacgctc tacgtgcaag gcgattacaa gggcgccgtc
    11701 cacgagctcg tcgccgcgta ctgcatctcg ccgtactaca cgatcctcaa ggacatcggc
    11761 caagcgtacg agcgcgagct cgattacgag aaggcgatcg cgtacctcga gcggttcgtc
    11821 gtcgcggtgc cgaaggatgc gcagcgcgcg aacagttgcg cgcccgatcc gcaagacgag
    11881 aagaagaacg tgatcgcacg catcaacgtg ctcggcgcgt tgaaggcgaa gatcctgatc
    11941 aacaccgatc cggccgactc gaaggtcacg ctgtcgaacg atgccggcat cgcggggcgc
    12001 ggtcgctcgg gccaggagct cgaggtcgcc ggtggccgtt acgaggtgct gatcgagcac
    12061 gacggctatc actcggtgac gcaggagatc cccgcgaaga tcggcaagcc gtacacgatt
    12121 ttcgcgaagc tcgaaccgct caaaggacgg ctgcgcgtgc gcgtcgtgcc ggcggacgca
    12181 cggctcttcc tcgacagcaa acaagtcggc gccggtgcct acgagggtga gctcgaaggc
    12241 aagcgctaca ccttgagcgc cgaggcgcct ggacgcatct cggtatcgcg cgagatcgag
    12301 gtgttcccga atcgcgacac accgatctcg ttcgagcttc cgccggagcc gcaagtcggt
    12361 cgccgccagc tcctcggata cgcaggcatc ggcggtgcgt tcgctggcgg cttcctcggc
    12421 gctgcgacga cgaacggcgg tttgatcgca ggtggtgtcg gcgccggtgc agcggcgggt
    12481 gtgctcggtg cgtacttcgg cacgccgaaa gatgtcccgc tcggcacgag ctcgctcacg
    12541 atcacgtcat cgctgatcgg cggcaccgcg gtgggcgcgg gcgcgttgct cgccaccgac
    12601 gatccgcaga aggtgctgcc cgcgatcggt ggtggtctca tcgcaggtgc gggcatcggc
    12661 tactacgcgg gctcgcggct gcgaattcga ccgggggatg ccgcggtgat caactccggt
    12721 gcattgtggg gcggcgtgac cggtggcttg ttcgtcggca gcttcgacgc cgggcggacg
    12781 gtcggctccg gcatcgtgct atcgggtctc ggcatgggca cgctcggtgg tgtgctgatg
    12841 acgcgctact tcaacgtgag ccgtggtcgt gccgcgctga tcgatgtcgg cggtacggtg
    12901 ggaatcttca tcggcatcgc gatcgaaaac gtcgtgacgc aggccaactg cgatgcgtcc
    12961 gatccgagtt gcaatcagag cgagcgtgag cgaaccgcaa actattcgct cggcggcatg
    13021 gctgccggcc tgatccttgc gggtgtgctg acgcgcaaca tggatgcgcc aaagctctct
    13081 gtgtccccgg taattagcaa gaccagtact ggtgccggcc cgtcgacgag cacattcgga
    13141 ttcggcggcc ggttctgatc ggctagagtg cgcctgtgga acaccggcgc agtgcatgtc
    13201 ctctcgattg ccccgacctg tgcgggttga cggtcaccgt cgataacggg cgggttgtcg
    13261 aggtcgacgg cgatcaccgc gcgccactca ccaacggctt tatctgcggc aaggtccgca
    13321 agatcgcgga gcacgtgcac ggcgacgcgc gcgtgctgca tccgatgatc cgcacgggcg
    13381 cgaagggtag cggccagtgg cgacaggtgt cgtgggacga agcgctcgat acgatcgcgg
    13441 aacgcatcca aacgatccgc gcgcgcgccg gctccgaagc gatcttgccg taccactacg
    13501 gcggctcgaa cggctggctc accgaaggcg cgctcgcgac ccggttcttc cgccggctcg
    13561 gcgcctcgaa tctcgatcgt acgttctgcg ccggcgcgac caccgcggca acgcacgggc
    13621 tctacggcaa gatggcgggc atcgcgctcg aagactacga gcacagcaag ttgatcgtgc
    13681 tctggggcgt gaatccgtcg gcaaccggca tccacctcgt tccagtgatc gagcgcgcga
    13741 tcgcgaacgg cgcgaagctc gtcgtcgtcg atccgcggcg gacgccgctc gcacggcgcg
    13801 ccgatctgca tctaccggtg cgcccgggtg ctgacttgcc agtcgcgctc gcgatcgtca
    13861 accaattgtt cgcgcgcggt catgcagacc tcgcattcct cgacaagcac gcggtcgacg
    13921 tggccgagct tcgcgagcgt gccggtgcgt ggtcgctcga ggcggcggcg cgtgaagcga
    13981 acgtcgatgt cgcggcgctc gatcggttcg tcgagctcta cgcgtccgcg tcaccggcgg
    14041 gcattcgcct tggctatggc ctcgagcgca accgcaatgg cggcagcgca gtcgctgcga
    14101 tcctcgcgat gcccgccgtc gcgggcaagt tcggcgtgcg tggcggtggc ttcacgatgt
    14161 cgaatggcga cgcgaagtgg acggtgagcg cggagatcgc gatcgatacg ccggcaccgc
    14221 cgacgcgtca catcaacatg agcgagatcg gtcgcgtgct cgccaccgta aacgatccgc
    14281 gcatcgagct gctgttcatc tacaactgca acccggtctc gaccgcaccc gatcagcgca
    14341 cgctcgtcga gcagctcgcg cgcgaggacc ggttcgtcgt cgtccacgag caagtctgga
    14401 ccgatacctg caatctcgcc gacgtcgtgc tgccggcgac gacgttcctc gagcatcgcg
    14461 agctgcgtcg gggctacggc aacatgcgca tgtacgacag ccccgcggtg atcgatccgg
    14521 tcggcgaggc acgcagcaac aaccagctgt tcggtgcgct gctgcgcaag ctcgacctcg
    14581 tgcgtcccgg tgacgcgatg accgacgacg agctggtagc gaagacgttc gccgccggcg
    14641 acggtgcagc gctccaggcg cagctcgccg agaagcacgt tgcccagatc cccggcaatg
    14701 cgcggccggt gatgttcgtc gacgagttcc cgagcacgcc cgatcgcaag atcca
//
LOCUS       DASUFX010000069         8038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_845456,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000069 DASUFX010000000
VERSION     DASUFX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845456"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFV99_02545"
     CDS             <1..560
                     /locus_tag="VFV99_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058206.1"
                     /translation="MNGATKDWIEVFTWAVGVVGALAGLLNLARETRQSRRQRSDELR
                     WRKASGANDMLNSFYADPVFRNAMLMLDWDGRPFQMDENRTITITREDIWAALARPPA
                     PFDDKNLFIRTAMDRMFEAFDRIGHAIKIEVMADRDITYALVYYVGVLAQNRDVVQKY
                     MLDLGFKEALTYFRKHQEWERLGQG"
     gene            599..1099
                     /locus_tag="VFV99_02550"
     CDS             599..1099
                     /locus_tag="VFV99_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058207.1"
                     /translation="MAEKLGFLDQMLWGKWADRAAINRNAEDLSNVEADVTALRTLVQ
                     RQAAEITQLRAMFMGMVEVLHQRGSLDETELESAVKAAFLKLSPPPPEPKPSASDPYR
                     GIATSEPTAEAIEAAKALLATAQKHHFGKRFDDARAVYQQIIDQYADTKQAATARQQL
                     ENLRKA"
     gene            complement(1108..1605)
                     /locus_tag="VFV99_02555"
     CDS             complement(1108..1605)
                     /locus_tag="VFV99_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058208.1"
                     /translation="MTQQHSIVFVACLGLVSAACSDNDTNLDTNDEDPEGGGTIELDL
                     GGGTDSFWADTDGVDPEVAGCHIEFTDDTCSTFAVPGRNFGEFCQNDGTLIESNPGKN
                     VCHTHEHDIGHPYVVDCYEWCTNMLVLPDGLRRGIPADDGECVVVHGLPCDGGIVESA
                     KCVCH"
     gene            complement(1768..2640)
                     /locus_tag="VFV99_02560"
     CDS             complement(1768..2640)
                     /locus_tag="VFV99_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5058209.1"
                     /translation="MTRLAYASDGETVAYRIHGAPGATPLITVHGLVSSIEHWQFFTP
                     YYAAKRPVVSWEYRGHGGRPAPRDPATVTIPHFAEDAYAVMRAAELPPSIVVGLSFGV
                     QVALELWRAHRDAVRALVLICGTAGHPLDRVSSSPLVRRFAADVVRGMASTRFLAEPV
                     LSFLRSKQGTKLACELAYLSGGAHRDACPDEVLQHLFAHAGALAPEVIGAITASYFEH
                     SAWDVLPTITVPTLIIAGDKDQLTPVATAERMHRAIPNSELVVFPGHSHLVQVEKPHE
                     VHAAIDAFLQRHAL"
     gene            complement(2642..4756)
                     /locus_tag="VFV99_02565"
     CDS             complement(2642..4756)
                     /locus_tag="VFV99_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5058210.1"
                     /translation="MKRLWLGVPLCLLALRADAVVTATWTVETYQQFDAGDAASAFIT
                     STGEVRPGWDTKRVKLEGDAIWSALRLNDGSVLLGSDANGTIYKVGESAKPFAKLDGA
                     IAVVSLAQTSDGTIWAGAMPGSKIWKIDSKGKATAGPALKDVETIWSLAAAGNTVYAG
                     TGPSGKLFAITGNSAKEVFDSEDKRITALTVTSDGAVWMGTSERALVFRYDPKDGKTR
                     AMADFAGNEVTAIAPYRTGVVVAANDLAETPPPTGKTTTQVEAAEKPSAAKGQAAKAP
                     DVGSKPGADKDPTPVTDLGRKGAKKGKGALFRVGGDGRLDQLHALTATYFTSVAVAPD
                     GTIYAGAADKGRVYMVDNDDSVGTAFDVDERAVSQLWFEKNLLGFTTDDAAALYRATG
                     RASQAKYMSDVLDAKAVARFGKLTWIAQGKVKIETRSGNTAKPGVGWSDWASPSQIGK
                     LGGGVDGGKITSPPGRYLQFRAALEDDDARVRRVSSFYVPQNSATDVQEITVEPSSKE
                     NLPTLKDLAAKARSPILKVKWKVENPDNDDTVYTLEARRDGEANWRPLQTGKAPLTAT
                     SWEWNTETYPDGWYKLRVTASDQAANSPDRALQSSKMSTLFAIDNTRPTIDGLQINVG
                     KGGATARATDQISTIAEMAFSVDDQPWQLGTTADGLFDDLTEDLRIDLPTGLSRGTHT
                     LAVRVADSAGNVGSTSSTFVVK"
     gene            complement(4771..6549)
                     /locus_tag="VFV99_02570"
     CDS             complement(4771..6549)
                     /locus_tag="VFV99_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEX5058211.1"
                     /translation="MKAGQTGYGLTTMSGTKPERFTFEVVSVVHNFLPKQDIILVKSD
                     DKKLAVTGFWQGMSGSPLYIDDKLVCAFSYGFRFNKVSLGGCTPIEYMKREGGTPRRA
                     NAVAQKGGGPKIVQQPAASMSDWKRLAPTVDPQAAMDALGPRHKSWLLSAPLPAPVPQ
                     PTETEGGVMTASVPLAVSGFSAPAFNQLEKLFGQSNIVPVRAGGTGGTSTGQSPEIGP
                     SKFEMGGSIAVQLIRGDMSAAATGTVSYVDGNNILAFGHPMFQTGETYAPVATSTVHT
                     VVPSAMSAFVLASPIKEIGSLTQDRQAAIAADTNLRTPPIPVSISIVTAAGKHSEKGQ
                     FNVEVLDNKFLTPAITGAALMNAVNYYLPDRDDVTAKVESTVKIKGGQPIEFTDYLYA
                     NDGASSVMGAVRGLRVLVPLMLNPYAPVQVESVDIKVDLRFEANYGDIKEIKVPSGDL
                     AVGRNTVQVLLSTWDGKDIVEEVPVDVPPALAGSIVQLEVTAGDSAKLDAAPPTDLPS
                     LLSAFRKLLPGNVWATTIYPADEGVALEGKLVKDLPASAMDKLHPQSHTQRAQSYKPL
                     VRTVAPAKRVVNGSASMLVRVRATTK"
     gene            6879..7106
                     /locus_tag="VFV99_02575"
     CDS             6879..7106
                     /locus_tag="VFV99_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058212.1"
                     /translation="MSNFVETWKGIATALGRSERWCRYMARRGADPLPVFKVGGIVRL
                     NNNDLEDWLSRQRDRSMRGPSPEDVALRLIA"
     gene            7135..7905
                     /locus_tag="VFV99_02580"
     CDS             7135..7905
                     /locus_tag="VFV99_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX5058213.1"
                     /translation="MTRPLSDLRGPVRALFSDVDGTMTTGDRIEAATYEALERVGEAG
                     VPVIMVTGRPAGFGHAFMKMTPVLACVAENGGVMFVREGRKLVKSYGVPAASLPEWRR
                     RMNDIAVDVMSKVPGARLSSDSKYREVDLAIDWNEEVQLSRDDAETCVQMIQKAGFAA
                     VRSSVHVNFGPPHFDKLSACQQVVRRVLGGDMSDLAPYVYVGDALNDAPMFHGFPTSV
                     GVANVRTWWDELAHKPAFITERPEGAGLRELVEHLLSL"
BASE COUNT         1320 a         2548 c         2718 g         1452 t
ORIGIN      
        1 acatgaatgg ggcgacgaag gactggatcg aggtgttcac gtgggcagtc ggcgttgtcg
       61 gcgcgctcgc tggcctcttg aacctcgccc gcgagaccag gcagagccgt cgtcagcgga
      121 gcgacgagct gcgctggcgc aaggcgagcg gcgcgaacga catgctcaac agcttctacg
      181 ccgatccggt gtttcgtaac gcgatgctga tgctcgattg ggatggccgg ccgtttcaga
      241 tggacgagaa caggacgatc acgatcactc gcgaggacat ctgggcagcg ctcgcgaggc
      301 caccggcgcc gttcgacgac aagaacttgt tcattcgtac ggcgatggat cggatgttcg
      361 aagcgttcga tcggatcggc cacgcgatca agatcgaggt gatggcggac cgcgacatca
      421 catacgcgct cgtgtactac gtcggtgtgc tcgcgcagaa ccgcgatgtg gtccagaagt
      481 acatgctcga cctaggcttc aaggaggcgc tcacgtactt ccgcaagcac caagaatggg
      541 aacggctggg gcaaggctga agcaccgcag cggcgattct tcgcgtactc ttgccgcgat
      601 ggcggagaag ctcggctttc tagatcagat gctgtggggc aaatgggcgg atcgcgcggc
      661 gatcaaccgc aatgccgagg acctgtcgaa tgtcgaagcg gacgtgaccg cgctccgcac
      721 gctcgtgcag cgccaggccg ccgagatcac gcagctgcgc gcgatgttca tgggcatggt
      781 cgaggtgctt catcagcgcg gcagcctcga cgagacagag ctcgagagcg cggtgaaggc
      841 ggcgttcctc aaactgtcgc caccgccacc cgagccgaag cccagcgcga gcgatccgta
      901 ccgcggaatc gccacgagcg agcccacggc cgaggcgatc gaggctgcaa aggcgctcct
      961 cgcgaccgcg cagaagcatc atttcggcaa acgcttcgac gacgcccgtg cggtgtacca
     1021 gcagatcatc gatcagtacg ccgacacgaa gcaggcagcg acggcacgcc agcagctcga
     1081 gaatctacgc aaggcgtgat gttgcactta gtggcagaca cacttcgccg attcgacgat
     1141 tccaccatcg cacggtaggc cgtgaacgac gacacactcg ccgtcgtcgg cggggatgcc
     1201 gcgccgcaat ccatccggta gcacgagcat gttcgtgcac cactcgtagc aatcgactac
     1261 gtagggatgc ccgatgtcgt gttcgtgcgt gtgacacacg ttctttcccg gattggattc
     1321 gatgagagtt ccgtcgttct ggcagaactc gccgaagttg cgacccggca ccgcgaaggt
     1381 agagcacgtg tcgtcggtga attcgatgtg acagccggcg acctctggat cgacgccgtc
     1441 ggtgtcggcc cagaacgaat ccgttccgcc tcccaaatcg agctcgatcg ttccaccgcc
     1501 ttcggggtcc tcgtcattcg tgtcgagatt cgtgtcgttg tcggagcaag ccgcagacac
     1561 gaggccaaga cacgctacga acacgatcga atgctgttgg gtcatgggag gctctcctcg
     1621 ggcagttctc cctaaccatg atgctgacgc gatgccggac aatcctcctc acgtcggcat
     1681 ctccccgaga gcgcgcgttg tgactgcgta cgacgaccgc agtacgcgac gtggtcgcgc
     1741 cgcgccacgt cgcagcgcga ctaccgctca gagcgcgtgg cgctgaagga acgcgtcgat
     1801 cgcggcgtgc acttcgtgcg gcttctcgac ttgcacgagg tgcgagtggc cggggaacac
     1861 gacgagctcg ctgttcggga ttgcgcggtg catgcgttcg gcggtcgcga cgggcgtcag
     1921 ctggtctttg tcacccgcga tgatcagcgt cggcacggtg atcgtcggca gcacgtccca
     1981 cgcgctgtgc tcgaagtagc tcgccgtgat cgcgccgatc acctcgggcg caagcgcgcc
     2041 ggcatgcgcg aacaggtgct gcagtacctc gtccggacac gcgtcgcgat gcgcgccgcc
     2101 gctgagatac gcgagctcgc aggcgagctt cgtgccttgc ttcgagcgca agaacgacag
     2161 cacgggctcg gcgagaaagc gcgtcgacgc catgccgcgc acgacgtccg ccgcgaagcg
     2221 acgcacgagc ggcgacgacg acacgcgatc gagcgggtgg ccggccgtgc cgcagatcag
     2281 cacgagcgcg cggacggcgt cgcgatgcgc gcgccacagc tcgagcgcga cctgcacgcc
     2341 gaacgagagg ccgaccacga tgctcggcgg cagctcggcg gcgcgcatga cggcatacgc
     2401 gtcctcggcg aagtgcggga tggtgaccgt ggccggatcg cgtggcgccg gacggccgcc
     2461 atgcccgcgg tattcccacg acacgaccgg ccgcttcgcg gcgtagtacg gggtgaagaa
     2521 ctgccagtgc tcgatcgacg agacgagccc gtgcaccgtg atgagcggtg tcgcgccggg
     2581 cgcgccgtgg atgcgatagg cgaccgtttc gccgtcgctg gcgtacgcga gccgggtcac
     2641 gtcacttgac gacgaacgtc gacgacgtcg agccgacgtt accggccgag tcggcgacgc
     2701 gcaccgcgag cgtgtgagtg ccgcgcgaca ggcccgtcgg cagatcgatg cgcagatcct
     2761 cggtgaggtc gtcgaacagg ccgtcggcgg tcgtgccgag ctgccacggc tgatcgtcga
     2821 ccgagaacgc catctcggca atcgtcgaga tctggtcggt ggcgcgggca gtggcgccgc
     2881 ccttgccgac gttgatctgc aggccatcga tcgtcgggcg cgtgttgtcg atcgcgaaca
     2941 gcgtcgacat cttgctcgac tgtagcgcgc ggtcgggcga gttcgccgct tggtccgagg
     3001 ccgtcacgcg cagcttgtac cagccgtcgg ggtacgtctc ggtgttccac tcccacgacg
     3061 tggcggtgag cggcgccttg ccggtctgca gcgggcgcca gttcgcttcg ccgtcgcggc
     3121 gtgcttcgag cgtgtagacg gtgtcatcgt tgtcggggtt ttcgaccttc cacttgacct
     3181 tgaggatcgg gctgcgcgcc ttcgccgcga ggtctttgag cgtgggcagg ttttccttcg
     3241 acgacggctc gaccgtgatc tcctggacgt cggtcgcgct gttctgcggc acgtagaatg
     3301 acgacacgcg acgcacgcgt gcgtcatcgt cctcgagtgc ggcgcggaac tgcaggtagc
     3361 ggccgggcgg cgaggtgatc ttgccgccgt cgacaccacc gccgagcttg ccgatctgcg
     3421 acggcgaggc ccagtcggac cagccgacgc cgggcttcgc ggtgttgccg ctgcgggtct
     3481 cgatcttgac cttgccctgc gcgatccagg tgagcttgcc gaagcgcgcg acggctttcg
     3541 cgtcgagcac gtcgctcatg tacttggctt gcgatgcgcg gccggtggcg cggtagagcg
     3601 ccgcggcgtc gtcggtcgtg aagccgagca ggttcttctc gaaccagagc tggctgaccg
     3661 cgcgctcgtc gacgtcgaag gcggtgccga cggagtcatc gttgtcgacc atgtacacgc
     3721 ggcccttgtc ggcggcgccg gcgtagatcg tgccatccgg cgcgaccgcg accgacgtga
     3781 agtacgtcgc cgtcagcgcg tgaagttgat cgaggcgacc gtcaccgccg acgcggaaca
     3841 gcgcgccttt acctttcttg gcacccttgc gaccgaggtc ggtgaccggg gtcggatcct
     3901 tgtcggcgcc cggcttgctg ccgacgtcgg gcgccttcgc agcctggcct ttcgcagccg
     3961 acggcttctc ggcagcctcg acctgggtcg tcgtcttgcc ggtcggcggc ggcgtctcgg
     4021 cgagatcgtt cgcggcgacc acgacgcccg tgcgatacgg cgcgatcgcg gtgacttcgt
     4081 tacccgcgaa gtcggccatc gcgcgcgtct tgccgtcctt cggatcgtag cggaacacga
     4141 gcgcgcgctc cgacgtgccc atccagaccg cgccgtcgct cgtgacggtc agcgcggtga
     4201 tgcgcttgtc ttcggagtcg aacacttcct tcgcgctgtt gccggtgatc gcgaacagct
     4261 tgcccgacgg gcccgtgccc gcgtagaccg tgttgcccgc ggcggcgagc gaccagatcg
     4321 tctcgacgtc cttgagcgcg gggccggcag tcgctttgcc cttcgaatcg atcttccaga
     4381 tcttcgagcc gggcatcgcg cccgcccaga tcgtgccgtc actcgtttgc gcgagcgaga
     4441 cgactgcgat cgcgccgtcg agcttcgcga acggtttcgc gctctcgccg accttgtaga
     4501 tcgtgccgtt cgcgtcggag ccgagcagca ccgagccgtc gttcaggcgc agcgcggacc
     4561 agatcgcgtc gccttcgagc ttcacgcgct tggtgtccca gccgggacgc acctcgccag
     4621 tcgaggtgat gaatgcactc gccgcgtcac ccgcgtcgaa ttgctgatag gtctcgacgg
     4681 tccacgtcgc ggtaacaacc gcgtctgcac ggagcgcgag caggcacaga ggcactccca
     4741 accagagtcg cttcacagat gtctcctgac ttatttcgtc gtcgcgcgga cgcgcacgag
     4801 catcgaggcc gaaccgttca ccacgcgctt ggccggagcc acggtgcgta cgagcggttt
     4861 gtaggattgt gcgcgctgcg tgtggctctg cggatgcagc ttgtccatcg cgctcgcggg
     4921 aagatctttg acgagcttgc cttcgagcgc gacgccctca tcggcagggt agatcgttgt
     4981 cgcccagacg ttgccgggca gcagcttgcg gaatgcagag agcagcgacg gcagatcggt
     5041 cggcggcgct gcgtcgagct tcgcgctgtc accggcggtg acttcgagct ggacgatgct
     5101 gcctgcgagc gccggcggca cgtcgaccgg aacctcctcg acgatgtcct tgccgtccca
     5161 cgtcgagagc agcacttgca ccgtgttgcg accgaccgcg aggtcgccgc tcggcacttt
     5221 gatctccttg atgtcgccgt agttcgcttc gaagcggagg tcgaccttga tgtcgacgct
     5281 ctcgacctgg accggcgcgt acgggttcag catcaggggc acgagcacgc gcaggccgcg
     5341 caccgcgccc atcaccgagc tcgcaccgtc gttcgcgtag agatagtcgg tgaactcgat
     5401 cggctggccg cccttgatct tgaccgtcga ctcgaccttc gcggtcacgt cgtcgcgatc
     5461 gggcaggtag tagttgaccg cgttcatcag ggctgcgccc gtgatcgccg gcgtgaggaa
     5521 cttgttgtcg agcacctcga cgttgaactg ccccttctcg ctgtgcttgc cggcggcggt
     5581 gacgatcgag atcgagaccg gaatcggcgg cgtgcggagg ttcgtgtcgg cggcgatcgc
     5641 agcctgccgg tcctgcgtga gcgagccgat ttctttgatc ggcgacgcga gcacgaacgc
     5701 gctcatcgcc gatggcacca ccgtgtgaac ggtcgacgtc gcgaccggcg cgtaggtctc
     5761 gccggtctgg aacatcgggt gaccgaacgc gaggatgttg ttgccgtcga cgtacgagac
     5821 ggtgccggtt gccgccgccg acatatcgcc gcggatcagc tgcaccgcga tcgatccgcc
     5881 catttcgaac ttcgatggac cgatctccgg cgactggccg gtcgacgtgc cgccggtacc
     5941 gcctgcacga accggcacga tgttcgattg gccgaacagt ttctcgagct gattgaatgc
     6001 cggcgccgag aaaccggaga ccgcgagtgg caccgacgcg gtcatcacgc cgccttcggt
     6061 ttctgtcggc tgtggaaccg gtgcgggcag cggtgcgctg agcagccagc tcttgtggcg
     6121 cgggccgagc gcatccatcg cggcctgtgg atcgacggtt ggcgcgaggc gcttccaatc
     6181 ggacatcgat gccgcgggct gctggacgat cttgggaccg ccacctttct gggcgaccgc
     6241 attcgcgcgg cgaggcgtgc cgccttcgcg cttcatgtac tcgatcggcg tgcagccacc
     6301 gagcgagacc ttgttgaagc ggaagccgta cgagaacgcg cagacaagct tgtcatcaat
     6361 gtagagcggg ctgcccgaca tgccttgcca gaagcccgtg accgcgagct tcttgtcgtc
     6421 gctcttgacc aggatgatgt cctgcttcgg caagaagttg tgcacgaccg agacgacctc
     6481 gaacgtgaac cgctccggct tcgtgccgga catcgtggtg agaccgtagc cggtctggcc
     6541 ggctttcact ttttcgaggg ggtatgtgtc tgggcctgcg agtgcgctcg cagacagcgc
     6601 caaacaagca cccaggaccg cggctccaag ccgcatgtgt ccacgcatgt tcaacaaaga
     6661 tacgcggcac ttctgcgttc tgccagtgtg ggaagcggcc gcggaggtgc ggaaaattag
     6721 cgacaactgt gtcagctctt gcgtcggttt tttctgacta gtgtctagtc gttgacaggg
     6781 tgatctccac cggatccatc gggtttgcca ggagttacac caaaggtccg accccggcac
     6841 gacctctgca caaagcggcg ggcacacgga ggacgtcaat gtcgaatttc gtcgaaacct
     6901 ggaaaggcat cgcaactgcc cttggccgct cggagcgttg gtgtcggtat atggcccggc
     6961 gcggagcgga cccgcttccg gtgttcaagg tcggcggcat cgttcggctg aacaacaatg
     7021 atctcgagga ctggctgtct cgccagcgcg atcgctcgat gcgtggcccg tcgcctgagg
     7081 acgtggcgct ccgcttgatc gcctgatcta gtcgcgtgct atagagcgcg cggcatgact
     7141 cgccctttgt ctgacctgcg cgggccggtc cgcgcgttgt tctcggatgt tgacgggacg
     7201 atgacgaccg gcgatcgcat cgaggccgcg acctatgagg ccctcgagcg agtgggtgag
     7261 gcgggagttc cggtgatcat ggtgaccggc cggcctgcgg gcttcggtca cgcgttcatg
     7321 aagatgacgc cggtgttggc gtgtgtcgct gaaaacggcg gcgtcatgtt cgtgcgcgaa
     7381 gggcgtaagt tggtcaagtc gtacggtgtg ccggcagcga gcttgcccga gtggcggcgt
     7441 cgcatgaacg acatcgccgt cgatgtgatg tcgaaggtgc cgggagcgcg actatcgagc
     7501 gactcgaaat atcgcgaggt cgatctggcg atcgactgga acgaagaggt ccagctgtcg
     7561 cgcgacgacg ccgagacctg tgtccagatg atccagaaag cgggcttcgc ggcagtgcgg
     7621 tcgagcgtgc acgtcaactt cggaccgccg cacttcgaca agctaagcgc ctgtcagcag
     7681 gtcgtacgtc gcgtgctcgg cggcgatatg agcgatctcg caccgtacgt gtacgtaggc
     7741 gacgcgctca acgatgcgcc gatgttccac ggttttccga cgagcgtcgg cgttgcgaac
     7801 gttcgcacgt ggtgggacga gctcgctcac aaaccagcgt tcatcaccga gcgcccagag
     7861 ggcgccggtc tgcgcgagct tgtcgagcac ttgctgtcgc tgtgacaccg gccgaggtca
     7921 tcgagcgcgt gttcacgagc gcgaacccga cgacgggctc gcagccgccg ccggccatgg
     7981 catgggtcgt gttcgcacat ggcaccgcgt tcttcaccgc gccgagcgac gacctgcc
//
LOCUS       DASUFX010000070        12214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1678576, whole genome shotgun sequence.
ACCESSION   DASUFX010000070 DASUFX010000000
VERSION     DASUFX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678576"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VFV99_02585"
     CDS             <1..1705
                     /locus_tag="VFV99_02585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX5058214.1"
                     /translation="DLIDLVRVDRGLAKGERRFPKKDTCLAIYSRTVNARTQLETVLA
                     ESYPWCREHAAALRTLFAAYVDEKLARHVLDYDDLLLWWWHAMSEPELAQAIRSRFDH
                     VLVDEYQDTNALQAEIVVALAPSGKGVTAVGDDAQAIYSFRAATVHNILDFPKVFATP
                     ARVVPLEENYRSTVPILAASNAVIERAPQRYAKHLRSQRPSEERPTLAIVDDELAEID
                     YVIEKVLAYREAGILLREQAVLMRAAHHADLLELELGRRRIPYVKYGGLKFLEAAHVK
                     DLLGILRWAENPVDPIAGFRTAQLVPGVGPAFARRAVDAVVAGGPATTGSMTRGRSGA
                     TPSLGALAELKPPAAARTAWPALCELLLALANPQTPWPGQIGRVRQFYEPLLVERYDG
                     VEARLGDLAQLEAIGGTYPARGGFLDDLTLDPPSVTGDYAGPPVIDEDWLVLSTIHSA
                     KGQEWKSVFILDIIDGRMPSDLAVGTPDQIDEERRLLYVAMTRARDHLHLVQPLRMFI
                     EKQHRHGDRHVYAPRTRFIPDAILDRFERVVRGRGVASVPGVDPPATTVDVASRLRAM
                     W"
     gene            1858..2559
                     /locus_tag="VFV99_02590"
     CDS             1858..2559
                     /locus_tag="VFV99_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5058215.1"
                     /translation="MTTIQVAGQPMRVSVRTPSASTNCGVVVMIHGPGLDAFIEKQVE
                     LLAAHGFIAASADLFHRQPDDGTDTMTKVSRLRDREIIEDVDALVAHMRELGAERLAV
                     IGFCMGGRNTYLLAGARPALWTCAGVFYGGNIMKPWGDGPSPFARTGQIACPMIGFFG
                     NDDTNPSPDDVTTIDAELSRLGKPHVFHRYDGAGHAFLNFTNPERHRPAQAADAWAKL
                     VAFLDDQFATSNVAR"
     gene            2717..4273
                     /locus_tag="VFV99_02595"
     CDS             2717..4273
                     /locus_tag="VFV99_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058216.1"
                     /translation="MLGGCLDFAGSDASDNDLTSVGGNEYVIDFDGFVDVTPGASDDV
                     VKDAIHRQLKSALGALREQGIGVSDRDAIRNLANPTLVRSHFDVVRTDGTVSHSLDRV
                     RYHYHDQALCEKGRCTGSTVDITLLFGDFVARASELIPLCSDDQSTEPDSLWYHYQPN
                     IASCKAAIVAEQRAIDHDRATLADMTAQITANDADRRFLPTRASLASVTAAPDLWPEH
                     DQLWGFAGDSARTKLVVYSFFGVDSDESKPSDLGLVEYLRFERELRKRMPKLRVTETN
                     PNAWLLDFYIGGQKVPNVTWDDVERWVVDGTGFPALVGNDATKKAELLRQVVTNFSER
                     WIVWSAPITVTRNGEQRTMTVEVRTWHGWEDGSPDIRLHARWRYLEAFWHADVFAYTG
                     HSHFGHGPLEPWEYSGSNFPDRYQTMLINSCLSFNYYDEDFLRMHPGGSTKLDVVVNG
                     LPAYWQGMGQATAAYVAGVTGGSQSWRQLLTAMRVNLPWQTGYDPMRAVNGELDNQFK
                     PQSGAITIVD"
     gene            complement(4270..4515)
                     /locus_tag="VFV99_02600"
     CDS             complement(4270..4515)
                     /locus_tag="VFV99_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058217.1"
                     /translation="MGHRLDVGDVIDMLEESAMMRRPMMVILTDGRTFEDRVQEIIKW
                     DGEDHVVFKDHELTPISNISDIRRGRPPEFTYAGKRV"
     gene            complement(4705..5160)
                     /locus_tag="VFV99_02605"
     CDS             complement(4705..5160)
                     /locus_tag="VFV99_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058218.1"
                     /translation="MVGKETEDWDPGTATNASIWPTTYGNPPVTITNTSVLVKGAPPD
                     SNGNPQFLAFVVWNTNFVGHIWLVKKGAEGVNFKGVMLNSFAAQTAGHAGFQSLFAGN
                     VDTDPPVPRGPGPTIDQYWIEQPELNNAKINSQTMIGAIENFTNFNGQQ"
     gene            complement(5363..8344)
                     /locus_tag="VFV99_02610"
     CDS             complement(5363..8344)
                     /locus_tag="VFV99_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5058219.1"
                     /translation="MTDCGQTERTHLLADGELSPAEAEAAREHLATCAHCQAELADIM
                     QLDAAVGDVAAKRGQVVSIAWYRKRNVQLVAAVVAMAAAVVIFLATRKHPPVDKPEQV
                     AIVLAPKRMTEARVTWDAAAKYREYDVPRGDVKHEQVGLAALAELEKRGDNHGIGVLA
                     LLEGDRKRAATYLEKVGETPDVLVDRAAIALGDGQPAVALALVDRAMEKAPQHGAALW
                     NRALALRDLGLVRSAAEAFRTVAKLGEPGWADEATQRAKAIDAEADVSVERFTRLVKA
                     GADLARGELTVSLDDARAQPGLARGITYDAIRSAATPEALDKLAPLAKAIDEADRDTA
                     MMDAIARAKTGLHPELSTAYAQIIRALAVEQQQLQNQADVPPVPRGATRAQLLANLRA
                     ARADDLLLGVLLKTSDDRVRVNASELAEFAKLAAASPDPWMQLLGLSQQAQAATGAND
                     LMGAEAILVRGKERCKAPGAPAYRCIQLQGNLGELYLKWQRLPEARATLEAAWAAARA
                     SNEWWLGVGLLPQLARLAVLGDDATGSGLPLLRAYNGETVLRVPPSNALERCQSDEWG
                     RTQRATVLINRLEFDAARRELAGPACAKPKTPGQAAQQLFVRAELATPADVAKLREDI
                     AAVRADKRATATDQLILDHIEGRAIIESDPPAGEALLERAIAAANAQPATALEARKMA
                     AYSYAVLAVAAAKRGDGAKALAMLAAEQGLTVPTTCVFGAVVEDRHQAFVARDAAGAV
                     HVHHDETRTVPQIDPATLVPAEITAALATCKEVDVIARPPVHGMARILGDKIAWRYLA
                     HRPSPIAPAAKRSLVVANVEPPAGLELPRLGTWQTGADETISGAAATPSRVLAAIASA
                     GEVVINAHGLVDAAQPEASFLALSPESDGRYALTVADVRKAKFTASPLVILAACRASQ
                     AAPVWHATWSLPTAFVYAGARAVIASASPIPDADANAFFDDVRARIRGGAAAAIAVRD
                     ARVAWLAQHRGDWVRDVIVFE"
     gene            complement(8341..8883)
                     /locus_tag="VFV99_02615"
     CDS             complement(8341..8883)
                     /locus_tag="VFV99_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5058220.1"
                     /translation="MTVAVAAWDRAPATMVREQAFAREVREHEAVLTAIARRLCANTA
                     DADDLVQETYERALRSLERYDDRGNLRSWLAAIMNNLFIDRCRKAKRAPQTEGIENLQ
                     VATPEPVAPPAWANVTQAEIRTALDRIGAEFRSVYELHASGLSYDDIARELGIPKNTV
                     GTRLIRARKKLKEILLEGLS"
     gene            complement(8932..9537)
                     /locus_tag="VFV99_02620"
     CDS             complement(8932..9537)
                     /locus_tag="VFV99_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5058221.1"
                     /translation="MSEPSPLLSDDAEVRMRILRRDHDGATVAAIRAYGPELIGWLNG
                     TLRSEADAHDAFSRMSEQLWRSLPSFDARCSVRAWCYMLARQAAARVRSGARHKHEVL
                     VSQVPSLGMAVSAAWSTRSRAERQLNNVYAEIRRELDEDDQTLLVLRVDRKLEWRDIA
                     QVILGEDATIEETTRSAAALRKQFERVKQRLKKLVAERIGE"
     gene            complement(9534..10880)
                     /locus_tag="VFV99_02625"
     CDS             complement(9534..10880)
                     /locus_tag="VFV99_02625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058222.1"
                     /translation="MDDEAGAVSTTDSIVRRIARAEVEPIAFAPGQVLGDHYELLERI
                     GGGGMGIVYRARDRRLDRDVALKVLRVQGNDDHVRRLFEREARATAQLLHPNIVTLHH
                     VGEHDGNPYLVLELLSGETLAARLARRGKLPVAEALVMLDGVLGALAFAHERGVLHRD
                     LKPHNVFVTGDERVKVLDFGVALSLDTSPGPVTRAAGTPGYMAPEQRDGGVQDARTDV
                     WAAALLLVECLTGHRPVDRDASHSVSALDAPPAVRTVLAQALSADPAQRHESAGQFRQ
                     ALARAAGARALDRAQRRRLGAIAAGAVVAAAAIATGDTNWVDHSQPKVGEPTAGEVSG
                     LWSTSYGTLDLKVAADGTAYGVYTHDSGIVMGHYEDGLFLGQWCELPSRRGPEDGGPL
                     QLWFTHSAHKLQLSGRWSYANLPTAQWRTDFSGSMIDDKPSSELVARFDQHELCPP"
     gene            11042..>12214
                     /locus_tag="VFV99_02630"
     CDS             11042..>12214
                     /locus_tag="VFV99_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HEX5058223.1"
                     /translation="MNGLMMMTALAGCLDTVDPAATDDHEPSADPSGNTEPEYGTLRI
                     RDAYGEVHAVTVKNVNGRAIYEGDMNLGDFARTKERAFASPVTVGNRWFGNVVRYQFQ
                     TTPAGCTSPLCSCEVMSGHLCHNLNWTEQWFIRTLMDKYTEETGIVFEESSTQPFISL
                     AGTDDPLASYTYADGDFHNLGFSPLITFSTSFASDDRVVLHELGHALGLTHEQKRNDA
                     DSTIKFNAICMEDPNQFDSAGSSFNYLSPFDDKSIMLYSSWTFARASTGCNTVTSNCC
                     PAILSKTGSPQKTFSYTNDTGKTIKVTFEGTQYIMNAGATTTFTGAIIDRFTDFSDED
                     LNAFYQMYPPNLGMNESGDRFGGHAAVGDFNGDGYKDLAVSAVTEGPNGHATSGAVY"
BASE COUNT         2041 a         4180 c         3879 g         2114 t
ORIGIN      
        1 ggacttgatc gatctcgtgc gcgtcgaccg cgggctggca aagggcgagc ggcgatttcc
       61 gaagaaggac acctgtctcg cgatctactc gcgcaccgtc aacgcgcgca cgcagctcga
      121 gaccgtgctc gccgagtcgt atccgtggtg tcgcgagcac gccgccgcgc tgcgcacgct
      181 gttcgcagcg tacgtcgacg aaaagctcgc ccgtcacgtg ctcgactacg atgatttgtt
      241 gctgtggtgg tggcacgcga tgagcgagcc cgagctcgcg caggcgattc gcagccgctt
      301 cgatcacgtg ctcgtcgacg agtaccagga cacgaacgcg ctgcaggccg agatcgtggt
      361 cgcgctcgca ccgagcggca aaggcgtgac cgcggtcggc gacgacgcgc aggcgatcta
      421 ttcgttccgc gcagcaaccg tccacaacat cctcgacttc ccgaaggtgt tcgcgacacc
      481 ggcgcgcgtc gtgccgctcg aagaaaacta tcggtcgacg gtgccgatcc tcgccgcgtc
      541 gaacgcggtg atcgagcgcg cgccgcagcg ctacgcgaag cacctgcgct cgcagcgccc
      601 gagcgaagaa cgccctaccc tcgcgatcgt cgacgacgag ctcgccgaga tcgactacgt
      661 gatcgagaaa gtactcgcgt atcgcgaggc cggcatcttg ctccgcgagc aggccgtgct
      721 gatgcgcgcc gctcaccatg ctgatctgct cgagctcgag ctcggccgcc gccgcattcc
      781 gtacgtgaag tacggcggcc tcaagttcct cgaagccgca cacgtcaagg atctgctcgg
      841 catccttcgg tgggcggaaa acccggtcga tccgatcgcc ggctttcgca ccgcacagct
      901 cgtgccgggc gtcgggccgg cgttcgcccg ccgcgccgtc gatgccgtcg tcgccggagg
      961 accggcgacg acgggatcaa tgacacgggg tcgctccggt gcaacgccgt cgctcggcgc
     1021 gctcgccgag ctcaagccgc ccgccgccgc acgcactgca tggcctgcgc tgtgcgagct
     1081 gctgctcgct ctcgcgaacc cgcagacgcc atggcccggc cagatcggcc gcgtgcgcca
     1141 gttctacgag ccgctactcg tcgagcgcta cgacggcgtc gaagcacgac tcggcgatct
     1201 cgcgcagctc gaggcgatcg gcggcacgta tccggcgcgc ggcggattcc tcgacgatct
     1261 cacgctcgat ccgccgagcg tcaccggcga ctacgccggg ccaccggtca tcgatgaaga
     1321 ctggctcgtg ctgtcgacga tccactccgc aaaaggccag gagtggaagt cggtgttcat
     1381 cctcgacatc atcgacggcc gcatgccctc ggacctcgcg gtcggcacgc ccgatcagat
     1441 tgatgaagag cgccggctgc tctacgtcgc gatgacccgc gcgcgcgatc acctgcacct
     1501 cgtccagccg ctgcgcatgt tcatcgagaa gcagcaccgg cacggcgatc gtcacgtcta
     1561 cgcgccgcgc acgcggttca tccccgacgc gatcctcgat cgcttcgagc gcgtcgttcg
     1621 cggccgtggc gtcgcgagcg taccgggagt cgatccaccg gcgaccaccg tcgatgtcgc
     1681 ctcacggttg cgtgcgatgt ggtgaccggg taccactgcg ggcaatggcc gactcggatc
     1741 cacgcgtcga gagcaagatg ggccgcctgc tcgttccgat ctttctcatc gtattcgcgg
     1801 cggcggtcgg catcatgatc tgggccgtcg tgacgatgta gcgagtagga tgcgcgcgtg
     1861 acgacgatcc aggtagctgg ccagccgatg cgggtgagcg tgcgcacgcc gagcgcgtcc
     1921 acgaactgcg gcgtcgtcgt gatgattcac ggccccgggc tcgatgcgtt catcgagaag
     1981 caagtcgagc tgctcgccgc gcacggcttc atcgctgcat cggcggatct atttcatcgc
     2041 cagcccgacg acggcaccga cacgatgacg aaggtaagcc gcctgcgcga tcgcgagatc
     2101 atcgaggacg tcgatgcgct ggtcgcgcac atgcgcgagc tcggagccga gcgcctcgcc
     2161 gtgatcggct tctgcatggg cggccgcaac acgtacttgc tcgcaggcgc acggcccgcg
     2221 ctgtggacct gtgccggcgt gttctacggc ggcaacatca tgaagccgtg gggcgatggc
     2281 ccctcgccgt tcgcccgcac cggccagatc gcgtgcccga tgatcggctt cttcggcaac
     2341 gacgacacga atccgtcacc cgacgatgtc acgacgatcg acgccgagct gagccggctc
     2401 gggaagccgc acgtcttcca tcgctatgac ggcgccggcc acgcgttcct gaacttcacg
     2461 aaccccgagc ggcatcggcc ggcacaagcc gccgacgcgt gggcgaaact tgtcgcgttt
     2521 ctcgacgacc aattcgcgac atcgaatgtt gctcgctaat gcagtggaag ctcgcgtagc
     2581 cacccttctc gttgcgaacc gcgacgcgct ccgacgacgt tctcgctaac tgctcataac
     2641 cgtttgcacg gtcgatggcg cagccgttgc ctatggcgcg gccgtgcgct tcgcttcgct
     2701 tagcttggtt cctctggtgt tgggtggctg tctggacttt gccggatcgg atgcgtccga
     2761 caacgacctg acgtcggtcg gcggcaacga gtacgtcatc gacttcgacg gcttcgtcga
     2821 tgtcacgccc ggcgcgtcgg acgacgtcgt caaagacgcc atccaccgcc agctcaagag
     2881 cgcgctcggc gcgctgcgcg agcaaggcat cggcgtgtcg gatcgcgacg cgattcgcaa
     2941 tctggcgaac ccgacgctcg tgcgctcgca cttcgatgtc gtgcgcaccg acggcaccgt
     3001 ctcgcattcg ctcgatcgcg tgcgctacca ctatcacgac caggcgctgt gcgagaaagg
     3061 tcgctgcacc ggcagcacgg tcgacatcac gctgctgttc ggcgacttcg tcgcgcgcgc
     3121 cagcgagctg attccgctgt gctccgacga tcagagcacg gagcccgact cgctctggta
     3181 ccactaccag ccgaacattg cgtcctgtaa agccgcaatc gtcgcggagc aacgagcgat
     3241 cgatcacgac cgcgcgacac tcgccgacat gacggcgcag atcacggcga acgatgccga
     3301 ccgccggttc ttgccgacgc gcgcgtcgct cgcgtccgtc accgccgcgc ccgacttgtg
     3361 gcccgagcac gatcagctgt ggggcttcgc cggcgattct gcgcgcacga agctcgtcgt
     3421 ctactcgttc ttcggcgtcg acagcgatga aagcaagccg agtgacctcg gcctcgtcga
     3481 gtacctccgc ttcgagcgcg agctgcgcaa gcggatgccg aagctacgcg tcaccgagac
     3541 gaacccgaat gcatggctgc tcgacttcta cattggcggc cagaaggtgc cgaacgtgac
     3601 gtgggatgac gtcgagcgct gggtcgtcga tggcacggga ttccccgcgc tcgtcggcaa
     3661 cgacgccacg aagaaggcgg agctactccg ccaggtcgtc accaacttct cggagcgctg
     3721 gatcgtatgg agcgccccga tcacggtcac gcgcaacggc gagcaacgca cgatgaccgt
     3781 cgaagtccgc acgtggcacg gctgggagga cggcagcccc gacatccgct tgcacgcgcg
     3841 ctggcgctac ctcgaagcgt tctggcacgc cgacgtattc gcgtacaccg ggcacagcca
     3901 cttcggtcac ggaccgctcg agccgtggga gtacagcggc tcgaacttcc cggatcgcta
     3961 ccagacgatg ctgatcaact cgtgcctgtc gttcaactac tacgacgaag acttcctacg
     4021 catgcaccca ggtggcagca cgaagctcga cgttgtcgtc aacggtctgc ccgcgtactg
     4081 gcagggcatg ggccaagcga ctgccgcata cgtcgctggt gtcaccggtg gctcgcagtc
     4141 gtggcgtcag ctactgacgg cgatgcgcgt gaacctgcca tggcagaccg gctacgatcc
     4201 gatgcgcgcc gtcaacggcg agctcgacaa tcagttcaag ccgcagagcg gcgcgatcac
     4261 gatcgtcgac tagacgcgct tacctgcgta tgtgaattcg ggaggtcgac cgcgtcggat
     4321 atcgctgatg ttcgagatcg gcgtcagctc gtggtccttg aacaccacgt ggtcctcgcc
     4381 gtcccacttg atgatttcct gcacccgatc ttcgaacgtt cgtccgtcgg tcaggatcac
     4441 catcatcggc cggcgcatca tggccgattc ttcgagcata tcgatcacgt ctccgacgtc
     4501 gagtcgatgt cccattggaa cctcctaccg tgaagcgtcc cagtgcgact cgttcacggc
     4561 aagcgtgcgc cgtcgtcaca tcaggagagc agcgttagca atactccgtc acggcacgcc
     4621 gccgccctac ccacccgaaa gctgggcaac acccgagctc cgccgggggt agaacgctac
     4681 ccccggcgag ccacgcccgc cgccctactg ctggccgttg aagttcgtga agttctcgat
     4741 cgcgccaatc atcgtctgcg agttgatctt cgcgttgttg agctccggct gctcgatcca
     4801 gtactggtcg atcgtcgggc ccggaccacg cggaaccggc ggatcggtgt cgacgttgcc
     4861 ggcgaagagc gactgaaaac cggcgtggcc cgctgtctgc gctgcgaacg agttgagcat
     4921 gacgcccttg aagttgacgc cttccgcgcc cttcttgacg agccagatgt ggcccacgaa
     4981 gttcgtgttc cagacgacga acgccaggaa ctgtgggttg ccgttcgaat cgggcggagc
     5041 gcctttcacg agcacggacg tgttcgtgat cgtcactggc ggattcccgt acgtcgtcgg
     5101 ccagatgctg gcattggtcg ctgtccccgg atcccagtct tcggtctcct tgccgaccaa
     5161 gtagctctcc gtttgcgacg tttgcgggtc agccccttga accgcacagc cggagatcgt
     5221 tgcgccgaga aatagaatcg aacaactcgc cagtctcatg tccacctctg ctgcgtttga
     5281 gctcacagag aggagtcaac gagccctgtg aatgtgacgg gcgcgcgcga acttttttcg
     5341 cctttggagt cgagaccttg cgctattcga acacgatcac gtcgcgcacc cagtcgccac
     5401 ggtgctgagc tagccacgcg acacgcgcgt cacgaaccgc gatcgcggcc gctgcaccgc
     5461 cacggatgcg agcccgcacg tcgtcgaaga atgcgttcgc gtcggcatcc gggatcggtg
     5521 acgccgaagc gatcaccgcc cgcgcaccgg cgtagacgaa cgccgtaggc agactccagg
     5581 tcgcatgcca caccggcgcg gcctgggacg cgcgacacgc tgccaggatc acgagcgggc
     5641 tcgctgtgaa ttttgccttg cgcacgtcgg ccacggtgag cgcgtagcgg ccatcggact
     5701 ccggtgacag cgcgagaaac gatgcttccg gctgtgcggc atcgacgagt ccatgcgcat
     5761 tgatcacgac ttcaccagcc gacgcgatcg cagcgagcac gcgtgacggc gtcgctgccg
     5821 caccgctgat cgtctcgtca gcgccggtct gccaagtgcc gaggcgcggc aactccaggc
     5881 cagcgggcgg ctcgacattc gcgaccacga gcgatcgctt cgcagcgggc gcgatggggc
     5941 tcggacggtg cgcgaggtaa cgccatgcga tcttgtcacc gagtatgcgc gccatgccat
     6001 gcaccggcgg tcgcgcgatc acatccactt ccttgcaggt cgcgagcgca gcggtgatct
     6061 cggcgggcac cagcgtcgcc ggatcgatct ggggcacagt ccgcgtttcg tcgtggtgca
     6121 catgcaccgc gccggcggca tcgcgcgcga cgaacgcctg gtgacgatct tcgacgacgg
     6181 caccgaacac gcacgtcgta ggaaccgtca acccttgttc agctgccagc atcgcgagcg
     6241 ccttggcacc gtcgccgcgt ttcgcagccg cgacggcgag cactgcatag ctgtaggccg
     6301 ccatcttgcg cgcttccaac gccgtcgcgg gttgcgcgtt cgctgcagcg atcgcgcgct
     6361 cgagcaacgc ttcgcctgcg ggcggatcgc tctcgatgat cgcgcgaccc tcgatatgat
     6421 cgaggatgag ctggtccgtc gccgtcgccc gcttgtctgc acgcactgcc gcgatgtctt
     6481 cgcgcagctt cgcgacgtcc gccggcgttg caagctccgc ccgaacaaag agctgctgcg
     6541 ccgcttgccc tggcgtcttc ggtttcgcgc acgcaggacc cgcgagctcg cgacgggcgg
     6601 catcgaattc gagccgattg atgagcacgg tcgcgcgctg ggtccgcccc cactcgtcgc
     6661 tctggcaccg ctcgagtgca ttggatgggg gaacgcgcaa aacggtctcc ccgttgtacg
     6721 cgcgtaacag cggtaggccg ctgccggtcg catcatcgcc gagcaccgcg agtcgcgcga
     6781 gctggggcag taatccgacg ccgagccacc actcatttga cgcacgcgct gctgcccagg
     6841 ctgcttcgag tgtggcccgc gcttcgggca accgttgcca tttcaggtag agctcgccga
     6901 ggttgccctg gagctggatg cagcggtacg ccggtgcgcc cggcgctttg caccgctcct
     6961 tcccgcgcac gaggatcgct tccgccccca tgaggtcgtt cgcgcctgtc gcggcctgcg
     7021 cctgctgtga cagaccgagg agctgcatcc acggatcggg agacgccgcc gcgagcttcg
     7081 cgaactccgc gagctcggat gcattgacgc gaacgcgatc gtcggacgtc ttgagcagca
     7141 cgccgagtag cagatcatcg gcgcgtgcag cgcgcaggtt cgccagcagc tgtgcgcgtg
     7201 tggcacctct cggcactggc ggcacatccg cctggttctg caattgctgc tgctcgaccg
     7261 cgagcgcgcg gatgatctgc gcatatgccg tcgagagctc gggatgcaga cctgtcttgg
     7321 cacgcgcgat cgcgtccatc atcgccgtgt cgcggtccgc ttcgtcgatc gccttcgcga
     7381 gcggcgccag cttgtcgagt gcctccggcg tcgcagccga gcgaatcgcg tcgtacgtga
     7441 tgccgcgagc gagccccggc tgcgcgcgtg catcgtctag cgagaccgtc aactcgccac
     7501 gagcgaggtc ggcgcccgcc ttcacgagac gagtgaagcg ttccacgctg acgtcggctt
     7561 ctgcatcgat cgctttcgcg cgctgcgtcg cctcgtcggc ccatccgggc tcgccgagct
     7621 tggcgacggt gcggaacgcc tccgccgcac tgcgcacgag gccgaggtca cgcagtgcga
     7681 gtgcacggtt ccaaagcgcc gcaccgtgtt ggggcgcttt ctccatcgca cgatcgacga
     7741 gcgcgagcgc tacagccggt tgaccgtcgc cgagcgcgat cgccgcgcga tcgacgagca
     7801 cgtccggggt ctcgccaacc ttctcgagat acgtcgccgc gcgcttgcga tcaccctcga
     7861 gcaacgcgag cacgccgatg ccgtggttgt cgccgcgctt ctcgagctcg gcgagcgcgg
     7921 ccagcccgac ctgctcgtgc ttcacgtcgc cgcgcggcac gtcgtattcg cgatacttcg
     7981 ccgcggcatc ccacgtgacg cgcgcctcgg tcatccgttt cggtgccagc acgatcgcga
     8041 cttgctccgg cttgtcgacc ggcgggtgct tgcgcgtcgc gaggaagatc acgaccgccg
     8101 ccgccatcgc aacaacagca gcgacgagct ggacgttgcg cttgcggtac cacgcgatcg
     8161 acaccacctg cccgcgtttc gccgcgacat cgccaactgc agcatccagc tgcatgatgt
     8221 cggcgagctc ggcttggcag tgcgcgcagg ttgcgaggtg ctcgcgcgct gcctcggcct
     8281 cggcgggcga tagctcgcca tcggcgagca ggtgcgtgcg ttcggtttga ccgcagtccg
     8341 tcatgacagt ccctcgagca ggatctcttt gagcttcttg cgtgcgcgaa tgagccgcgt
     8401 cccgaccgta ttcttcggga tgccgagctc gcgtgcgatg tcgtcgtacg agaggccaga
     8461 cgcatgcagc tcgtacacgc tgcgaaactc cgcgccgatg cgatcgagcg cggtacgaat
     8521 ctcggcctgc gtcacgttcg cccacgccgg tggggccact ggttcgggcg tcgcgacctg
     8581 gagattctcg atgccctcgg tctgtggcgc tcgcttcgcc ttgcgacagc gatcgatgaa
     8641 caggttgttc atgatcgcgg cgagccagct gcgcaggttg ccgcgatcgt cgtaccgctc
     8701 gagcgagcgc agcgcgcgct cgtacgtctc ctgcacgagg tcgtctgcgt cagcggtgtt
     8761 ggcacatagc cgtcgcgcga tcgcggtgag cacggcctcg tgctcgcgca cctcccgggc
     8821 gaacgcttgc tcccgaacca tcgtcgcggg ggcacggtcc catgcggcaa ctgcgacggt
     8881 caagtcacct ccttcgtctg ggtaacgacg gggggcgacg atcttcccga gctactcgcc
     8941 gattcgttcg gcgaccagct tcttgagccg ctgcttcacc cgctcgaact gcttgcgaag
     9001 tgcggcggca ctacgcgtcg tctcctcgat ggtcgcgtcc tcgccgagta tcacctgcgc
     9061 gatgtcacgc cactcgagct tgcgatcgac gcgcaggacg agcagcgtct gatcatcttc
     9121 gtcgagctcg cgacggatct ccgcgtagac attgttgagc tgccgctccg cgcgcgagcg
     9181 cgtgctccac gcggcgctga ccgccatccc gagcgatggc acttgcgaca cgagaacctc
     9241 gtgcttgtgc cgcgcgccac tgcgcacgcg cgccgcggct tggcgggcga gcatgtaaca
     9301 ccacgcgcgc accgagcacc gcgcatcgaa gctcggcaag ctgcgccaca gctgctccga
     9361 catccgcgag aacgcatcgt gtgcatccgc ttcgctgcgc agcgtgccat tgagccagcc
     9421 gatcagctcc ggaccatatg cgcggatcgc cgcgaccgtc gcgccgtcgt gatcgcggcg
     9481 caggatgcgc atgcgcactt cggcgtcatc ggagaggagc ggtgatggct cgctcatggc
     9541 ggacacagct cgtgttgatc gaagcgcgcg acgagctcgc tcgaaggttt gtcgtcgatc
     9601 atcgagcccg agaagtcggt gcgccattgc gccgttggca ggttcgcgta cgaccatcgc
     9661 ccgctgagct ggagcttgtg cgcgctgtgc gtgaaccaca gctgcaacgg cccgccatcc
     9721 tcgggcccac ggcggctcgg caactcgcac cactgaccga ggaagagacc gtcttcgtaa
     9781 tgccccatta cgatccccga gtcgtgcgtg tacacgccgt acgcggtgcc gtcggccgcc
     9841 actttgagat cgagcgtgcc gtaggaggtc gaccacagcc ccgacacctc gcctgccgtc
     9901 ggctcgccga ctttcggctg cgagtggtcg acccagttcg tgtcgccggt cgcgatcgct
     9961 gctgcagcga ccacagcgcc cgcggcgatc gcgccgagcc gccggcgttg cgcacgatcg
    10021 agtgcacgcg caccagccgc gcgagcgagt gcttgccgga actgccccgc agactcgtga
    10081 cgctgcgctg ggtctgccga cagcgcttgc gcgagcaccg tgcgcactgc cggtggagca
    10141 tcgagtgccg acaccgaatg cgacgcgtcg cgatcgacgg gacgatgccc ggtaaggcac
    10201 tcgaccagca gcagcgccgc ggcccacacg tcggtgcgcg catcctgcac gccgccgtcg
    10261 cgctgctcgg gcgccatgta tcccggcgtg cccgccgcgc gcgtgaccgg tcccggcgag
    10321 gtatcgagcg agagcgcgac gccgaagtcg agcaccttca cacgctcgtc gccggtcacg
    10381 aacacgttgt gcggcttcag atcgcgatgc agcacgccgc gctcgtgcgc gaacgcgagc
    10441 gcaccgagca caccgtcgag cattaccagt gcttcggcga ccggcagctt gccgcgccgg
    10501 gccagccgtg ccgcgagcgt ctcgcccgag agcagctcga gcacgaggta cggattgccg
    10561 tcgtgctcgc cgacgtggtg aagggtgacg atgttgggat ggagcagctg cgccgtcgcg
    10621 cgtgcttcac gttcgaacag cctccggacg tgatcgtcgt tgccctgcac gcgaagcacc
    10681 ttgagcgcga cgtcgcgatc gagccggcgg tcacgcgcgc ggtagacgat acccatcccg
    10741 ccgccaccga ttcgctcgag cagctcgtag tgatcgccga gcacctggcc gggagcgaat
    10801 gcgatgggtt cgacctcagc gcgcgcgatg cgccggacga tcgagtccgt cgtcgacacc
    10861 gcgccagcct cgtcgtccac agcgactgcc atcataaacg tttggctggg catccgactc
    10921 gataacgacg ggccgcggaa aacttcccgg tcttttttcg ctgggaatat gacgcgatcg
    10981 cgacgttctc ccgccggcga caggtttcgc cgaaacggag actcacatgc gatttgctgc
    11041 aatgaacgga ctgatgatga tgacggcgct cgccgggtgt ctggacacgg tggacccggc
    11101 ggcgaccgac gaccacgagc ccagcgccga tccctcaggg aacaccgagc ccgagtacgg
    11161 gacgctgcgc atccgcgacg cgtacggcga ggtccacgcg gtcacggtga agaacgtgaa
    11221 cggccgcgcg atctatgaag gcgacatgaa ccttggcgat ttcgcccgga ccaaagaacg
    11281 cgcgttcgca tcgccggtca ccgtcggcaa ccgctggttc ggcaacgtcg tgcgctatca
    11341 gtttcagacc actccggccg gttgtacgtc accgctgtgc tcctgcgagg tgatgtctgg
    11401 tcacctgtgc cacaacctca actggacgga gcagtggttc atcagaacgc tgatggacaa
    11461 gtacaccgag gaaacgggca tcgtcttcga ggaatcctcg acgcaaccgt tcatctcgct
    11521 cgcaggcaca gacgaccccc tcgcgagcta cacgtatgcc gatggcgact ttcacaacct
    11581 cgggttttcg ccgctcatca cgttttcgac gtcgttcgct tcagacgatc gcgtggtgct
    11641 tcacgagctc gggcacgcgc tcggcttgac ccacgaacag aagcgaaacg acgcggactc
    11701 gacgatcaag ttcaacgcga tctgcatgga ggacccgaat cagttcgact cggcgggcag
    11761 cagcttcaat tacctgtcgc ccttcgatga caagtcgatc atgttgtaca gctcgtggac
    11821 gttcgcgcgc gcgagcacgg gctgtaacac agtcacctcg aactgctgtc ctgcgatcct
    11881 gtccaagacg ggctcgccgc aaaagacgtt ctcgtacacg aacgacaccg gcaagaccat
    11941 caaggtgacg ttcgaaggca cgcagtacat catgaacgcg ggcgccacga cgacgttcac
    12001 gggtgccatc atcgaccgct tcaccgactt ctccgatgaa gacctcaacg cgttctacca
    12061 gatgtatccg ccgaacctcg gcatgaacga aagcggcgat cggttcggcg gtcacgcggc
    12121 ggtcggtgac ttcaacggcg atggctataa ggatctagcc gtctcggctg tgaccgaggg
    12181 ccccaacgga cacgctacgt caggtgccgt ctac
//
LOCUS       DASUFX010000071         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1123027, whole genome shotgun sequence.
ACCESSION   DASUFX010000071 DASUFX010000000
VERSION     DASUFX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123027"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFV99_02635"
     CDS             complement(<1..330)
                     /locus_tag="VFV99_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX5058224.1"
                     /translation="MKLLRGIVCLCLLASVAEAGSKMLKGPYLQDLAPSSITVMWQMD
                     ELVPAKLVVEGPGGERTQTVAPARIVEARIENLQPATRYRYRVEVGDDVWKGEFATAP
                     ETGKDVPF"
     gene            complement(405..>2233)
                     /locus_tag="VFV99_02640"
     CDS             complement(405..>2233)
                     /locus_tag="VFV99_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058225.1"
                     /translation="HYSTLDEGALYRFSWIFPAQKMGRGGIGFGGKETIESLESFAYL
                     PDDQIDARLGDELRDHPLLLVPVDERQKLIGKMLETSPNRAFQVGDYLRTGSLSPRNR
                     GIYDALLANYQGDYVQVLRHVRIERFEVSSRYRHGWVTVEPQLSVDATERQITADRTL
                     AALPPSLQSVSLHEYGGEVVGANRGMIEFDDLLKRPLEHYKYLLTTVERAALSMQSAT
                     LLLDLVFVGSCNDIHLAAFREIPDFQSFKGRIELVRVPFILDVRHEQALYRERLREAA
                     GGRHVAPHTAYVAALWAVLSRMRKPQIERFPSTLAEVIGKLTPLDKVELYSTGQVPED
                     LSPDRARELHAHIKELFHESDTYPIYEGRVGASPREMQTVLLAAAGSQRYEYVSPLVV
                     LDEIHELCKQASLYEFLRQDVQPGGYHDHKKLADVAKGKLFDVIDDEVRAAVGLVEES
                     EYARVFERYVTHVMHAIKKEKVRNPSTGRMEDPDEGMMREVERTLEIPGRADDFRQSL
                     IAKIGAWSLEAPNRGKKPIMTEIFADLLRKLKDAYFEKHKKTIAKGISDLVMLLSGNE
                     GHLSSEARTRADTALATMITRFGYTRESARDMVGALASMRYR"
BASE COUNT          361 a          741 c          741 g          390 t
ORIGIN      
        1 gaacggcacg tccttgccgg tctccggtgc cgtcgcgaac tcgcctttcc agacgtcgtc
       61 gccgacttcg acgcgatagc gatagcgggt cgcgggctgg aggttttcga tgcgcgcttc
      121 gacgatgcgt gccggcgcaa ccgtttgcgt gcgctcgccg ccgggtccct cgacgacgag
      181 cttcgcggga acgagctcgt ccatctgcca catcacggtg atcgacgacg gcgcgaggtc
      241 ctgaaggtac ggtcccttca gcatcttcga tcccgcctcc gccacactcg cgagcaggca
      301 gaggcacacg atcccgcgca gcagcttcac cagggaacag gatacggaat ggccggatgt
      361 aggcgcaagt tcgctgaagc gttgcgttcg ggtacgaccc ggcgtcagcg atatcgcatc
      421 gaggccagcg cgcccaccat gtcccgtgcg gactcccggg tatagccaaa ccgcgtgatc
      481 atcgtggcca gcgcagtgtc agcccgcgta cgagcctcgc tggacaagtg gccctcgttg
      541 cccgacagga gcatcacgag gtcggagatc cccttggcga tggtcttctt gtgcttctcg
      601 aaataggcgt cttttagctt ccggagcagg tcggcaaaga tctccgtcat gatcggcttc
      661 ttgccgcggt tcggcgcctc gagcgaccag gcgccgatct tggcgatcag gctctgccgg
      721 aagtcgtccg cgcggcccgg gatctcgagg gtgcgctcga cctcgcgcat catcccctcg
      781 tccggatcct ccatgcggcc cgtcgacggg ttccgcacct tctccttctt gatcgcatgc
      841 atcacgtgcg tgacgtagcg ctcgaacacg cgcgcgtact ccgactcctc gaccaggccg
      901 accgccgcgc gcacctcgtc gtcgatgacg tcgaacagct tccccttcgc gacgtcggcg
      961 agcttcttgt ggtcgtggta gccgcccggt tggacgtcct ggcgcaagaa ctcgtacagg
     1021 ctggcctgct tgcagagctc gtggatttca tcgagcacga cgagcggcga cacgtactcg
     1081 tagcgctgcg agccggcggc cgcgagcaac acggtctgca tctcgcgcgg cgacgcaccg
     1141 acgcggccct cgtagatcgg gtacgtgtcg gactcgtgga acagctcctt gatgtgcgcg
     1201 tgcagctcgc gcgcgcgatc aggtgacagg tcctcgggca cctgccccgt cgagtacagc
     1261 tcgaccttgt cgagcggcgt cagcttgccg atgacctcgg cgagcgtcga cgggaagcgc
     1321 tcgatctgcg gcttgcgcat gcgcgacagc acggcccaga gcgctgcgac gtacgcggtg
     1381 tgcggcgcga cgtgacggcc gccggcggct tcgcgcagcc gctcgcggta cagcgcttgt
     1441 tcgtggcgaa catcgaggat gaacggcacg cgcacgagct cgatgcggcc cttgaacgac
     1501 tggaagtccg ggatctcgcg gaacgcagcg aggtggatgt cgttgcagct gccgacgaac
     1561 acgagatcga gcaagagcgt cgcgctctgc atcgacagcg cggcgcgctc gacggtggtg
     1621 agcagatact tgtagtgctc gagcgggcgc ttcagcagat cgtcgaactc gatcatgccg
     1681 cggttcgcac cgacgacctc accgccgtac tcgtgcagcg agacgctctg cagtgacggc
     1741 ggcagcgcgg cgagcgtgcg atcggcggtg atctggcgct cggtcgcgtc gaccgacagc
     1801 tgcggctcga cggtgaccca gccgtggcgg tagcgcgacg acacctcgaa gcgctcgatc
     1861 ctcacgtggc gcagcacctg gacgtagtcg ccctggtagt tcgcgagcag cgcgtcgtag
     1921 atgccgcggt ttcggggcga cagcgagcca gtgcgcagat agtcgccgac ctggaacgcc
     1981 cggttgggcg aagtctcgag catcttgccg atcagcttct ggcgctcgtc gaccggcacg
     2041 aggagcagcg ggtgatcgcg cagctcgtcg ccgaggcgcg cgtcgatctg atcgtcgggc
     2101 aagtacgcga acgactcgag cgactcgatc gtctccttgc cgccgaagcc gatgccgccg
     2161 cgccccatct tctgcgccgg gaagatccaa ctgaagcgat acagcgcgcc ctcgtcaagc
     2221 gtcgagtagt gct
//
LOCUS       DASUFX010000072        19613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_845929,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000072 DASUFX010000000
VERSION     DASUFX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19613
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845929"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1256
                     /locus_tag="VFV99_02645"
     CDS             162..1256
                     /locus_tag="VFV99_02645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5058226.1"
                     /translation="MWSIVHVLSSFGVGGQERVALDLAIGQQARGHRVAVISLAPPPD
                     GAMADEFGEHGIAVGRIAKRDGIDLTLVPRLARALRRHRADVVHTHNPLPLIYGAPAA
                     RLAGAAAIHTKHGANPGGRGHRILRRAAAQLAHAFVAVSDTTFDQAMAQRDAPVARMH
                     TIANGIRLDRYHPDPEARAAARVELGLGDAWVVGTVGRLDPYKNQALLVRAMAPLLSS
                     DVRLVIIGDGHARGEVEAEIAKLREPRWVVMTGRRMDVPRLIPAFDVFALSSKTEGLP
                     LVVPEAMAAGLPIVTTAVGGLPGVVDDGKTGLLSPVDEGALRSALAKLEDDRQLARVM
                     GAQARTTALDRYSADRMVEAYLDLYREART"
     gene            complement(1253..1660)
                     /locus_tag="VFV99_02650"
     CDS             complement(1253..1660)
                     /locus_tag="VFV99_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058227.1"
                     /translation="MSPRIDVEALCWEVIGDRERSALLVDVSTEGARVERPYIGGRIE
                     YEVPLQIEVPGIDEVMWAKGDVVFDKLVPAKNGASNGGPFGLLRRTGYRIVIAAARDL
                     RLLRDYVWDMHNAQRARDTELEDLGMASCYLRG"
     gene            complement(1716..2774)
                     /locus_tag="VFV99_02655"
     CDS             complement(1716..2774)
                     /locus_tag="VFV99_02655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5058228.1"
                     /translation="MREHFARLLHRAGALAAMMNLRRHVPGPSTVSILTYHHIADEDA
                     AYPYDAGVADATPQQFRRQMELLARYGTPIGVDELIRALGGAQLPKNPVMVTFDDGYR
                     SCHDVALPILRAVGMRATFFVATGYINERKLYWWERVALCLSQARVPAAIITYPKTVE
                     VASRHPHSHRVLLDLIKDTPNLDIKRFIDELCAAFGVEWSDEIEATHAGNLIMSWDHV
                     RGLSRAGMDVESHSRWHRVLQTLDDGQLRDELEGSKHDLETQLGRPCRAIAYPVGRRI
                     SHLARIRNAVKAAGYRIGLSNASGATRIWPGSLGEVMPSDPFDIHRVSTDREMSDSMF
                     LAQIALPQLAYIGRHDSD"
     gene            2813..4192
                     /locus_tag="VFV99_02660"
     CDS             2813..4192
                     /locus_tag="VFV99_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5058229.1"
                     /translation="MFALPGIGLLIVFILARPQEFWPLLQKVPFLHLGAALAVLGYII
                     DVRLHRLQPIATNTLPWVIGFFTWCLISIAINAPEQLPHTAIEMAILFALYGTIAHGI
                     QRFRTMQAVMGVLAATCAFIAVVCVHQGLSARQCVGGQGVVGDIEGHPDGRPCETYLQ
                     CLGPDAEPGYEYRCERVGLFDTYSVDGRVRYMGELHDPNEIALTISAGGIGVLIAFAL
                     RKRRGGGKLAMVLGVLLMVTMVWMTKSRGGLIAAMLVPTVYVIRRFGIRAVIPMAMVA
                     IPVLLLGGRSDSSAEESTLMRYEAWSSGLEMVKQHPFVGVGPRMFTDHHYITAHNTYV
                     LTLAELGIVGMFLFVSLLFLSFKTLYVGIKELAKVPGTAAAQVWGLALLAGLAGITFQ
                     INTLSFAYHSVMWLMFGLIGAWYSTVRYHRPELRVRMTLRDVVVVAVLVFGYAFVALP
                     AFLKYKGEL"
     gene            complement(4254..5132)
                     /locus_tag="VFV99_02665"
     CDS             complement(4254..5132)
                     /locus_tag="VFV99_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058230.1"
                     /translation="MRSEFVEQLEPWFAASPRRSIVRDDDEEEESDDDEDEDEDEDED
                     EDEGEDEDGDDEPDDDDEGVDEVSDDWVGGGAGGGAADDEDEDWEESKDDKDEDEDED
                     DKDTGGWRDGDEPPWGADEDDEDEDEDEDEDEVSAEGDDEDEEDEEEDEDEDEDEEPA
                     PRPRPTLVRDDDEDEEESEDEDEDEDREEIDPEDRPRRGPLPPRPAKPLPTGKPEPIK
                     VPAKPAAGKPTPAPAKPAKPEPAPAKAPAAKAPAKPAAKPAAKAPAKPAAKAPAKKPA
                     AKAPAKKPAPPAKKKK"
     gene            5435..6613
                     /locus_tag="VFV99_02670"
     CDS             5435..6613
                     /locus_tag="VFV99_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058231.1"
                     /translation="MVQELTAETDIFYYFGRTDIDVTFRGDWKAFFLPTPRTEASLAA
                     DASYGQINALSASIVSNENPLQVMPTGNTNTANVAASEGASWVATEFVSLFQRGFARY
                     TTTDNNDPMTDVSTRSFEGGAGIGFERRMRYDSFSLEAGASYVYFKKNDPFFRQMGPR
                     LDEQVNPRMVGVWAHDFNKKWSAALNGGAVYVYPTFNLTGDTKPALFPTYGLTGAYTD
                     VWGRAQVFAGRVVTPNLFIAENTVADSLNAAAAMPLTFLDKDSRKRAPKVVGIGSAGV
                     ERTQLIDPTNGELTGSFLVARVDAAVAWQPRSGQTLGMRAELTYQDGDTIGEMVVPSY
                     HRFTFYFTYALRWPEDVGVRVPRRNNSVRADKSDLAPIGAEPVVIDPADLLEEGQGDR
                     "
     gene            6657..7043
                     /locus_tag="VFV99_02675"
     CDS             6657..7043
                     /locus_tag="VFV99_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5058232.1"
                     /translation="MERQLISSGSPYEHHIGLSRAVRVGPIIAVSGTAPLHVDGTTAH
                     IGDVYLQTKRCLEITLNAVERAGGARENVIRTRIMLVDIAHWKEAARAHGEVFATIRP
                     ACTFVQISRFIDPEWLVETEADCYVP"
     gene            7040..8086
                     /locus_tag="VFV99_02680"
     CDS             7040..8086
                     /locus_tag="VFV99_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058233.1"
                     /translation="MIDPSTFERCERATLVFDLAQIERTMQRVADAARATGIVPLFAL
                     KSFPHPRVRALAAQHLAGFDVASLGELHEAPPVSILSIVDPTGAAIAHAPKTARVIVG
                     CETVDHVRAAPAHAEIAIRVSASITERDPAVGALLEGSGRRRSRFGLDDREHIAALVH
                     AAAGRPIGVHVHHGPVTATNAERFIATARAALALFDHEPAFIDLGGAWHAITDLDAAF
                     AEIRAALPSALEILVEPGRLYAHDAGFATGRVLATREVGDRTLRVVELSRICHLRWSQ
                     VELVARAPRPGEGRKVQLVGPTCYEDDTIGEWIVEPADVAERVVLRNVSGYALAWNTS
                     FGGVPAADVVVVNA"
     gene            complement(8396..8722)
                     /locus_tag="VFV99_02685"
     CDS             complement(8396..8722)
                     /locus_tag="VFV99_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5058234.1"
                     /translation="MTFDCQRCGACCVNLPSNRAEGFVYWVEIADGDAILERADLVRK
                     HVVHDAGGVPHLRIVDDGRCLALRGALGRDVSCGIYHHRPSPCRTVQPGDALCLRYRD
                     EHGIES"
     gene            complement(8719..9387)
                     /locus_tag="VFV99_02690"
     CDS             complement(8719..9387)
                     /locus_tag="VFV99_02690"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX5058235.1"
                     /translation="MDELWPRIKKRWRAWLRAVHRDFGYLAIGFTVIYAISGIAQNHI
                     EDWGEVSYKSYEKTIHIAAIPDDAPDDVAIKAVADAAALGNPTSTYRAGDEVRLEYGS
                     GAKATAIGTDVTIQGRERRAFIGAANWLHTARNKKGWRYVSDVYAVLLLYLAISGIFM
                     IKGKLGLKWRGALLISMGVAVPVIAVLSSGPDARAKPAATETATAPAKDDGGIKFLPP
                     DQQP"
     gene            complement(9380..10108)
                     /locus_tag="VFV99_02695"
     CDS             complement(9380..10108)
                     /locus_tag="VFV99_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5058236.1"
                     /translation="MSDSPDTDRFPELKKALDRRNFIKSAAAGTIAMAFGGALFRIAA
                     DDLTRQARAEKRGDGRARLPPGQRVIEFLRPMGGQEGDGDVKTFKLKVHGLVKKPFEV
                     DYAGLLKLPQVTKAADVHCVTGWTMLGGEYKGVQIAKLAEIAEMTGEARYVILEAAHG
                     YTANVPLKEATTDNALITYRLNGKPFELKHGAPVRGLIPDLYFWKSAKWITGVKFVKD
                     DAPGYWEVRGYHNHADPWKEERYG"
     gene            10221..10589
                     /locus_tag="VFV99_02700"
     CDS             10221..10589
                     /locus_tag="VFV99_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058237.1"
                     /translation="MGRPSRFDIDWQRLGRWMLLGTIVMLLWLLAPVAKCSWYAFRDT
                     PIGEVNEDDLPGQSDKERVEQGTGFFSQWGTAIKGCYAQTPLFGQEPWKGKLLIGFGV
                     MTVLARTIGYIERRNKRTYS"
     gene            complement(10586..11497)
                     /locus_tag="VFV99_02705"
     CDS             complement(10586..11497)
                     /locus_tag="VFV99_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058238.1"
                     /translation="MRIWVALGLCAGCSFEHGTSVSGELPDAGDAVDVDGRMVDATPG
                     VFPTGPFGMPELVPISSANYRDDDITLTGDMLEIFFESDRVTLGNGDIYTSRRASVND
                     AWSLPARIDELSTQWIETSMEVSTDGLTMYFSSNRPPSTSVDVYITTRPDRSSAWTSP
                     VLVSSLSGSGGDWNAQPWSDTVLFLGSDRSPARGGSDIFRSTRASAGASWGTPGVIAG
                     LDTNRYEGEAFADTTGAIWFTGDAEGDDDIWRAEAVGDGSYKTPQLVLEIDTQYGEND
                     PWLSPDGHTLYFTSTRNGSLDIYVAKR"
     gene            complement(11500..12180)
                     /locus_tag="VFV99_02710"
     CDS             complement(11500..12180)
                     /locus_tag="VFV99_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5058239.1"
                     /translation="MNIVVTGATGALGAGLVAVLLERGADVYVPMVEPELPKYLAWRD
                     SARVHASPKVSLDDEAQVTAFYASVPNLWASIHLVGGFAMAPIAETSLADFEKQWRLN
                     TATCFLTCREAVKAIRKAGTGGRIVNVAARPVLVPTAGMVAYVASKAGVAAITQALAV
                     ELLAENILVNAVLPSVIDTPANRAAMPNADFASWPKPAQIAQTIAFLSSSDNALTTGT
                     LVPVYGHA"
     gene            complement(12180..13055)
                     /locus_tag="VFV99_02715"
     CDS             complement(12180..13055)
                     /locus_tag="VFV99_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5058240.1"
                     /translation="MRGDGDDDDTGSGSGSGSGSGSGEARQCDKMDIVFIVDNSGSMQ
                     EEQSNLASNFPMFAQLLDQYTVNNGMPLDYRVALTTTGRDVDYEIDLGGGFGTFPQSE
                     QGQNGEFQNNCSNTKRWLDRSDANMASTLSCRANVGTTGPSIEMPLLMAKWALKDRAV
                     DTNAGFLRDDALLAIVVLTDEDDASTTDNNFTLDASGMSPTNWNPQDEVSFLDGLKGN
                     RTRWAAGVIAGDGNCSSSFGSAADGVRLKQFVTLANGNGSTQAVFSSICDGNLTTGLK
                     EALDTFQAACGAIIL"
     gene            complement(13273..13965)
                     /locus_tag="VFV99_02720"
     CDS             complement(13273..13965)
                     /locus_tag="VFV99_02720"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5058241.1"
                     /translation="MGAQWKHAGRLASSAAKGKVFSRLAKEIIVAAKGGPDPALNARL
                     RAAVEAAKKASMPKDTLERAIKKGAGLLDEKANYENVVVEGFAPHRVPVIVECLTDNS
                     KRTMTNVRHVFRNGQLTPVAWDFTHVGIVDATPSGEADSEEAAIEAGAQDFEPDGEGG
                     VRFYTEPNDVDAVSKALSTRGWTVATMRIGWRAKNPVKLDDAAARAEVEKFLAELDED
                     DDVQHIYVGLEA"
     gene            14028..15761
                     /locus_tag="VFV99_02725"
     CDS             14028..15761
                     /locus_tag="VFV99_02725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058242.1"
                     /translation="MPHQVTGREPTEELAHEGEHTQIDSTEIGTQAESPKALSDAAAQ
                     GSLHATEHAPARPSGWATTSNNRTPHPTAAQAFDKSDLARSNMFHIFGICAPLGALVM
                     SLLIGGDETARYAFWIGAGMISLGNVGLVYFTSKPERYNATVVGLLWIFATIGVQPAV
                     YYFGPFSAVVMVDMLGIVFIAIGRVRWMARATAIVCIGGHLVIATPIIAGWIPDVGIM
                     ASIAATNEQMIVAEVLIVVFLTSGYFIGRWARSTASSAFRELQSAMRIIGDREQALQE
                     VVDEAARQKRVNEGRWTGQKLGTWRLGVVLGRGAMGEVYEAVGPKDRPAAIKLLNARS
                     TASTSIVERFHREMDVAARLESPHIVHVYEVSTPDAPVPYIAMELLQGVDLATKLRNE
                     NQNRMPSDELVVLIDQVARGLEVARLAGVVHRDLKPHNLFHHDGSVWKILDFGVSKVL
                     DSEGTLTGEGIVGTPQYMAPEQASGGQVTHLADVYALGAIAYRCLTGRSPFKGKDLAE
                     LVYQVVHTPPARPSTLGRVSQSIEDVLAVAMAKDPRRRFTSAMSFAQAFIAARRGRKV
                     AIDIPDNAWVL"
     gene            15834..17465
                     /locus_tag="VFV99_02730"
     CDS             15834..17465
                     /locus_tag="VFV99_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058243.1"
                     /translation="MIRRHVLAALAAGVEAGDDEAAREVLRGTDWVLRAPAQRELSRA
                     LIDAALLSGEFGASDRESVRHIHRVAALNLAKRFEVDVSNREDVAAAYATLNAAPPAR
                     APIATIFAGSAVALGVLLLVWLAVAIRRPSHPARPIPPLVTGAYFYGGTPARDEALEQ
                     YVVNELTDLVIETDAERGGSHDGAPRAKHAAELRNSSVIASRGPALAAAWRALIDTFD
                     HWVDVRVRGKEFRSVESELGRRAQAVSDQFAALGLGLYLQSDVMLDNGVAHAAIFVFA
                     VEEVVYVRAGGEPRRVLSLRRLDELNLRHTLLGRQGDELGDPVVLLDQIDEFVNARVL
                     PTLQGDVYPVGDEAWRSTIYGRGIAYRAGEAIRTELLAAVGTGSIEELRPKVARIIAA
                     SVRRHEARHGIDNERSTPLRFPRQLAAYVDNDGGPMALRARAELAGYLSQIGNEPVTP
                     QFALWNLASLTFNRTRWQSAEFYAGVVVIEGLARELGIGDGPTVKRGQFDRQRLVALA
                     EPLAAQSSDKLRAAARNLWIELYGEPMLPIIDVAR"
     gene            complement(17462..18067)
                     /locus_tag="VFV99_02735"
     CDS             complement(17462..18067)
                     /locus_tag="VFV99_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058244.1"
                     /translation="MRSGFVSAFVALALASGCVTVTSPRPLPPPRPVAPQPGAPPVQR
                     AADLPIPTTAPLVDERYVQWTLPVDLLAVIPLTYFMVQPDRYYLAAPSLLLPPLIHVA
                     YGNSESATISVLMRVAMVGGVYLAGRSAEDECNNSESFVCLPMRSILLGETAMILPIM
                     IDSFYLAKRTRGDDNWNRLPIVPSVAPMPGGGMTFGAATQF"
     gene            complement(18048..18413)
                     /locus_tag="VFV99_02740"
     CDS             complement(18048..18413)
                     /locus_tag="VFV99_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058245.1"
                     /translation="MRGMIVVLGLALAACTVDIARDVDLKYANARVPYQTDPAAVQVY
                     ETDVAVPYDVLGDLEVVVRQKNAFSEAPTKEHAIAALRAQAGRIGAHAIVLVDFGKQG
                     MSWWSYNELRGHGRAIRFR"
     gene            18520..18699
                     /locus_tag="VFV99_02745"
     CDS             18520..18699
                     /locus_tag="VFV99_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058246.1"
                     /translation="MRLLIRAVVTGFGLALGSALFKEARTRLGWDEEDDDSNAKRQEK
                     VNQQDGATDPGFQRS"
     gene            18831..19346
                     /locus_tag="VFV99_02750"
     CDS             18831..19346
                     /locus_tag="VFV99_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5058247.1"
                     /translation="MPQLADPNFSRAVVLMIEHSEQGSFGLVINHPSPIKASELLDSL
                     EMSWRGEDSAVVWAGGPVSPSTGWVLHEPVGGTQPGTIEITSTISLSTSPDRLRAIAN
                     QPPRNVRLLLGYSGWGPGQLAQEMARGAWLHTAADPRIVFETPPDEIWDRAMKSLGIN
                     PRDLFTGRGVN"
     gene            complement(19343..>19613)
                     /locus_tag="VFV99_02755"
     CDS             complement(19343..>19613)
                     /locus_tag="VFV99_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5058248.1"
                     /translation="MQIKVNFLCRDSILAAPIVLDLALFMDFAHRAGMSGIQEWLSFY
                     WKSPMTPEGLYPEHDLFIQLMKLKNTLRYTKGDELITHLGAEYYD"
BASE COUNT         3209 a         6500 c         6364 g         3540 t
ORIGIN      
        1 acgcggcctg cacgtcaccc atgcgtggcc catcgagtcg catcccaatc gacgccagca
       61 cagcgtcatc gtcgcccgaa ggggcaggag cgggagcggc ggtgtccgtc atgacgagcc
      121 caccttgcca ctccgtatcg caacgcggta gccaatctca cgtgtggtcg atcgtgcacg
      181 tgttgtcgtc atttggcgtc ggcggccagg agcgcgtcgc gctcgacctc gcgatcggtc
      241 agcaagcgcg cggccaccgc gtcgccgtga tctcgttggc gccaccgcca gatggcgcga
      301 tggccgacga gtttggcgag cacgggattg cggtcggccg catcgcgaag cgagacggga
      361 tcgacctcac actggtgccg aggctcgcgc gcgcactgag gcgccaccgc gcagatgtgg
      421 tgcatacgca caacccgttg ccgctgatct atggcgcacc ggcagcgcga ctcgcgggtg
      481 cggcagcgat ccacacgaag cacggcgcga atccaggcgg gcgcggtcac cggatcctgc
      541 ggcgtgccgc cgcgcagctc gcgcatgcgt ttgtcgcggt gtctgacacg acgttcgacc
      601 aagcgatggc gcagcgcgat gctccggtcg cacgcatgca cacgatcgcg aacggcatcc
      661 gcctcgatcg ctatcacccc gatcccgaag cgcgtgctgc ggcgcgggtc gagctcgggc
      721 tcggtgatgc gtgggtcgtc gggaccgtcg gccggctcga tccgtacaag aaccaagcgt
      781 tgctggtgcg cgcgatggcg ccgctgctct cgtcggacgt gcgactcgtg atcatcggcg
      841 acggtcacgc gcgtggcgag gtcgaagcag agatcgcgaa gctgcgcgag ccgcgctggg
      901 tcgtgatgac cggtcggcgc atggatgtac cgcggctgat tcccgcgttc gacgtgttcg
      961 cgctgtcctc gaagaccgaa ggactgccgc tcgtggtgcc cgaggcgatg gctgcagggc
     1021 taccgatcgt gacgaccgcc gtcggcggat tgccgggcgt cgtcgacgac ggcaagaccg
     1081 gtctgttatc gcccgtcgac gagggcgcac tgcggagcgc gctcgcgaag ctcgaagatg
     1141 atcgccagct cgcacgcgtg atgggcgcgc aggcacgcac gacggcgctt gatcgctaca
     1201 gcgccgatcg catggtcgag gcgtacctgg atctctaccg cgaagcgcgg acctaaccgc
     1261 gcaggtagca ggacgccatg ccgaggtctt cgagctcggt gtcgcgcgcg cgttgcgcgt
     1321 tgtgcatgtc ccagacgtag tcgcgaagca ggcgcaggtc gcgcgcggcc gcgatcacga
     1381 tgcgatagcc cgtacggcgg agcagaccga acggaccacc attggacgcg ccgttcttgg
     1441 ccggcacgag cttgtcgaag acgacgtcgc ccttcgccca catcacttcg tcgataccgg
     1501 gcacttcgat ctggagcggc acttcgtact cgatgcggcc gccgatatag ggccgctcga
     1561 cgcgggcgcc ctcggtcgac acatcgacga gaagtgcgct gcgctcgcga tcgccgatca
     1621 cctcccagca caaagcctcg acatcgatgc ggggactcac tcgacgaaga tcgcgcatac
     1681 ggaaaatttt cggctgccgc gcgccgcgga tccaattaat cgctatcgtg ccggcctata
     1741 taagcgagct gtggcagcgc gatctgggcc aggaacatcg agtcggacat ctcacggtcc
     1801 gtcgataccc ggtggatatc gaatggatcg gagggcatca cctcgccaag cgagcccggc
     1861 cagattcgcg tcgcgccgct cgcgttcgac agcccgatcc ggtacccggc tgccttcacg
     1921 gcattgcgaa ttcgcgcgag atgcgagatg cggcggccga ccggatacgc gatcgctcgg
     1981 catggacggc cgagctgcgt ttcgagatcg tgtttactgc cttcgagctc gtcgcggagc
     2041 tgcccatcgt cgagcgtctg cagcacgcga tgccagcgcg agtgcgactc gacatccatg
     2101 cccgcacgcg acaggccgcg cacgtgatcc cacgacatga tcaagttgcc ggcgtgcgtg
     2161 gcttcgatct cgtcgctcca ttcgacgccg aacgctgcac acagctcgtc gatgaagcgc
     2221 ttgatgtcga gattcggcgt gtccttgatc aagtcgagaa gcacgcgatg cgagtgtgga
     2281 tgtcgcgacg cgacctcgac cgtcttcgga tacgtgatga tcgcggccgg cacgcgcgct
     2341 tgcgacagac acagcgcgac gcgctcccac caatacagct tgcgctcgtt gatgtaaccg
     2401 gtcgcgacga agaacgtcgc tcgcattccg accgcgcgga gaatcggcag tgcgacgtcg
     2461 tgacacgatc gatacccatc atcgaacgtg accatcactg gattcttcgg cagctgtgct
     2521 ccgccgagtg cgcggattag ctcatcgaca ccgatcggtg tgccgtagcg cgcgagcagc
     2581 tccatctggc gacgaaactg ctgtggggtc gcgtctgcga cacccgcatc gtatggatat
     2641 gccgcgtcct cgtcggcgat gtggtggtac gtgaggatgc taacggtcga cggacctggg
     2701 acgtgacggc gcaggttcat catcgcggcg agcgctccag cgcgatggag caggcgggcg
     2761 aaatgttccc tcacggatgc gaaggatagc gtgggtgcta cggttcgcga gcgtgttcgc
     2821 gcttccaggc atcggtcttc tgattgtctt catcctggct cgcccgcagg agttctggcc
     2881 gctgcttcag aaggtgccgt ttctccatct gggtgcggcc cttgcggtac tcggttacat
     2941 catcgatgta cgtcttcacc gcctacagcc gattgctaca aacacgctgc cgtgggtgat
     3001 cggattcttc acgtggtgcc tgatttcgat cgcgatcaac gcgcccgagc agctgccgca
     3061 cacggcgatc gagatggcga tcttgttcgc gttatacggc acgatcgcgc acggcatcca
     3121 acgcttccgc acgatgcaag cggtgatggg tgtgctcgcg gcgacgtgcg cgttcatcgc
     3181 ggtcgtgtgc gtgcatcaag gcttgtccgc tcgccagtgc gtcggcggcc agggtgtcgt
     3241 cggcgatatc gaaggtcatc ccgacggccg gccgtgcgag acgtatctgc agtgcctcgg
     3301 ccccgatgcc gagccgggct acgaataccg ttgcgagcgc gtcggtctct tcgacacgta
     3361 ctcggtcgac ggccgcgttc gttacatggg tgagctgcac gatccgaacg agatcgcgct
     3421 gacgatctcc gcgggcggca tcggcgtcct gatcgcgttc gcgctgcgca agcgacgcgg
     3481 cggcggaaag ctcgcgatgg tgctcggtgt cttgctcatg gtgacgatgg tgtggatgac
     3541 gaaatcacgc ggtggactga tcgccgcgat gctcgtgccg accgtctacg tgatcagacg
     3601 tttcggcata cgcgccgtga tccccatggc catggttgcg ataccggtgt tgttactcgg
     3661 tggccgcagc gactcgagtg ccgaggagtc gacgctcatg cgctacgagg cgtggtcgtc
     3721 gggcctcgag atggtgaagc agcacccgtt cgtcggtgtc gggccgcgca tgttcaccga
     3781 tcaccactac atcacggcgc acaacacgta cgtgctcacg ctcgccgagc tcggcatcgt
     3841 cggcatgttc ttgttcgtgt cgctgttgtt cctgtcgttc aagacgctct acgtcgggat
     3901 caaggagctc gcgaaagtgc cgggcaccgc ggcagcgcag gtgtggggcc tcgcgctgct
     3961 cgccggtctc gccggcatca cgttccagat caacacgctc tcgttcgcgt accactcggt
     4021 gatgtggctg atgtttggat tgatcggcgc gtggtattcg accgtgcgct atcaccgccc
     4081 cgagctgcgc gtgcgcatga cgttgcgcga cgtggtcgtc gtcgccgtgc tcgtgttcgg
     4141 ctacgcgttc gtcgcgttgc cggcgttcct caagtacaag ggagagctgt agacccgctg
     4201 taacgccgca aggcccggcg ccctcacgca gatctcatcg acgagatcag cggctacttc
     4261 tttttcttcg cgggcggcgc cggcttcttc gcgggcgcct tcgcagcggg cttcttcgcg
     4321 ggcgccttcg cagcgggctt cgcaggcgcc ttggcggcgg gcttcgcggc cggcttagcg
     4381 ggcgcttttg ccgcaggtgc cttggcaggc gcaggctctg gcttcgcggg tttggcgggc
     4441 gccggtgttg gcttgccggc tgcaggtttc gcgggaacct tgatcggctc gggcttacct
     4501 gttggtagcg gtttcgcagg acgcggcggg agtgggccgc ggcgcggacg atcctccgga
     4561 tcgatctcct cgcggtcctc gtcctcgtcc tcgtcctcgg actcttcctc gtcctcgtcg
     4621 tcgtcgcgga caagcgtcgg gcgcgggcgt ggcgccggct cctcgtcctc gtcctcgtct
     4681 tcgtcttcct cttcgtcctc ttcgtcttcg tcgtcgcctt cggcggagac ctcgtcttcg
     4741 tcctcgtcct cgtcctcgtc ttcgtcgtcc tcgtcggcgc cccacggcgg ctcgtcaccg
     4801 tcgcgccagc cgccggtgtc cttgtcgtct tcgtcctcgt cttcgtcctt gtcgtccttg
     4861 gactcttccc agtcctcgtc ctcgtcgtcg gcagcaccgc caccagcgcc gccgccgacc
     4921 cagtcgtcgc ttacttcgtc gacgccttcg tcgtcgtcat ccggctcgtc atcgccgtct
     4981 tcgtcttcgc cttcgtcttc gtcctcgtcc tcgtcttcgt cctcgtcctc atcgtcatcg
     5041 gactcctctt cctcgtcgtc atcacggacg atgctgcggc ggggggaagc ggcgaaccat
     5101 ggttcgagct gttcgacgaa ttcactacgc atgatggaag cctttggacg cgattctaaa
     5161 gctcggcaac gtgaggtctg tcaacccgga gtgtggaact ttgctttcgg aaacgcactg
     5221 ctacaagtca cccgtgcttc ggcttttcgg ttgtagcgcg atctggtgcc tgatcggcac
     5281 tggcaccgcg cacgccgaca atgccgagat ccatgccctg gcctccggga gcgtggccac
     5341 cactgacaac gcgaacggct ccgcgacgga cccgcgcgga tccgtcttca ctgatgtccg
     5401 acccggcatg ctgtttacgt acaacgctcc gcggatggtg caagagctca ccgcggagac
     5461 ggacatcttc tattacttcg ggcggaccga tatcgacgtc accttccgcg gcgactggaa
     5521 ggcgttcttc ctacccaccc cgcgcacgga ggcgtcgctc gccgcggacg cgagctacgg
     5581 ccagatcaat gcgctctctg cctcgatcgt gtcgaacgag aatccgctcc aggtcatgcc
     5641 gaccggcaat accaacacgg cgaacgttgc tgcgagcgaa ggagcgtcgt gggttgcaac
     5701 cgagttcgtc tcactgtttc agcgaggttt tgcccgctac acgacgaccg acaacaacga
     5761 tccgatgacc gacgtatcga cgagatcgtt cgagggcggc gccggaatcg gcttcgagcg
     5821 ccgcatgcgc tacgacagtt tctcactcga ggccggcgcc tcgtacgttt acttcaagaa
     5881 gaacgatccg ttcttcaggc aaatggggcc gcgcctcgac gagcaggtga acccgcgcat
     5941 ggtcggtgtg tgggcgcacg acttcaataa gaagtggagt gcggcgctca acggcggcgc
     6001 ggtctacgtc tacccgacat tcaacctgac cggtgacacg aagccggcgt tgtttccgac
     6061 gtacgggctc accggtgcgt acaccgacgt gtggggccgc gcgcaggtct tcgccggccg
     6121 tgtcgtgacg ccaaacctct tcatcgccga gaacaccgtc gccgacagcc tgaacgcagc
     6181 ggctgccatg ccgctcacgt tcctcgacaa ggattcacgc aagcgagcac caaaggtcgt
     6241 tggcatcggc tccgccggcg tcgagcgcac gcagctgatc gatccgacaa atggcgagct
     6301 caccggcagc ttcctcgtcg cgcgcgtcga cgctgccgtc gcctggcagc cgcgctccgg
     6361 ccagacactc ggtatgcgtg ccgagctcac ctatcaggac ggtgacacca tcggcgagat
     6421 ggtcgtgccg tcctatcatc gcttcacgtt ctacttcacg tacgcgttgc gctggcccga
     6481 ggacgttggc gtgcgcgtac cgcgtcgcaa caacagcgtg cgcgccgaca agtccgacct
     6541 cgcaccgatc ggtgccgagc cggtcgtcat cgatccggcc gacctgctcg aagaaggcca
     6601 aggcgaccgc taagctcgcg accgcttgcc cagccgctcg gctagagttg cagccaatgg
     6661 aacgccagct catctcgtcg ggctcgccgt acgagcacca catcgggctc tcgcgggcgg
     6721 tccgggtcgg acccattatt gcggtctcgg gcaccgcacc cttgcacgtc gatggcacga
     6781 ccgcgcacat cggcgacgtc taccttcaaa cgaaacgctg cctcgagatc acgctgaacg
     6841 cggtcgaacg cgcgggcggc gcacgcgaga acgtgatccg cacgcgcatc atgctcgtcg
     6901 atatcgcgca ctggaaggaa gcggcccggg cacatggcga ggtattcgcg acgatccgac
     6961 ccgcatgcac gttcgtgcag atcagtcgct tcatcgaccc cgaatggctc gtcgagaccg
     7021 aagcggactg ctacgtcccg tgatcgatcc gagcacattc gagcgttgcg agcgggcgac
     7081 gctcgtgttc gatctggcgc agatcgagcg cacgatgcag cgagttgccg acgctgcgcg
     7141 ggcgacgggc atcgtgccgc tgttcgcgct caagtcgttt cctcatccgc gtgtccgcgc
     7201 gctcgcagcg cagcacctag ccgggttcga tgtcgcctcg ctcggcgagc tgcacgaagc
     7261 accgccggtg tcgatcctat cgatcgtcga tccgaccggc gccgcgatcg cccacgcgcc
     7321 aaaaactgcc cgcgtgatcg tcggctgcga gaccgtcgac cacgtacgcg cggcgcccgc
     7381 gcacgccgag atcgcgatcc gcgtgtcggc gtcgatcacc gagcgtgatc cagcggtcgg
     7441 cgcactgctc gaagggagcg gccgtcgtcg ctcgcgcttc ggcctcgacg atcgcgagca
     7501 cattgccgcg ctcgtgcatg cagctgcggg ccgcccgatc ggcgtccatg ttcaccacgg
     7561 cccggtcacc gcgacaaacg cagagcgatt cattgcgacg gcgcgtgcag cgctcgcgct
     7621 gttcgatcac gagcccgcgt tcatcgacct cggtggcgcg tggcacgcca tcaccgatct
     7681 cgacgcagca ttcgccgaga ttcgcgctgc actaccgagt gcgctcgaga tcctcgtcga
     7741 gcccggccgc ctctatgccc acgacgcggg cttcgcgacc ggacgcgtgc tcgccacgcg
     7801 cgaggtcggc gatcgcacgc tgcgcgtcgt cgagctgtcg cgcatctgcc acttgcggtg
     7861 gagtcaggtc gagctcgtcg cgcgcgcgcc gcgaccgggc gaaggccgca aggtccagct
     7921 cgtcggcccg acctgctacg aggacgacac gatcggcgag tggatcgtcg agcccgctga
     7981 cgtcgccgag cgcgtcgtgc tgcgcaacgt cagtggctac gcactcgcgt ggaatacgag
     8041 cttcggcggt gtaccggccg cagacgttgt cgtcgtgaac gcgtgaacgc gtgccggcgt
     8101 gggcgtcgag gatcgcgcca agcggcacgg tcttcctcac gccacttcga tgcgcaaggg
     8161 cagaccccta tatgtagagg gtctcctgtg ccgaagtggt acgaacggcg ttagtacgag
     8221 ccgcggccga gcgctgccgg acgaccaagg caacgtcatg gcccgatcgc catggcccga
     8281 tcctcatgcg ccatcgcgac gcgcgccgta ctaaccgcgt acgtaccact tcggcgggtg
     8341 acaccctctc catatacccg cctccggcct tcgcgccgcg gcggacggct tacgcttacg
     8401 actcgatgcc gtgctcgtcg cgatagcgca ggcacagtgc gtcacccggc tgcacggtgc
     8461 ggcagggcga cggacggtgg tgatagatgc cgcacgagac gtcgcgaccg agcgcaccgc
     8521 gcagcgcgag acagcggcca tcgtcgacga tgcgcaggtg cggcacgcca ccggcatcgt
     8581 gcacgacgtg cttgcgcacc agatcggcgc gctcgaggat cgcgtcgccg tccgcgatct
     8641 cgacccaata gacgaatccc tcggcacgat tcgacggcag attcacgcag caggcaccgc
     8701 agcgctgaca gtcgaacgtc acggctgctg atcgggcggc aagaacttga tgccgccgtc
     8761 gtcttttgcc ggcgccgtcg cggtctcggt tgccgcgggt ttcgcgcgcg catcgggccc
     8821 cgacgacagc acggcgatca ccggcactgc gacgcccatg ctgatcagca gcgcgccgcg
     8881 ccacttgagg ccgagcttgc ccttgatcat gaagatgccg ctgatcgcga gatagagcag
     8941 cagcaccgca taaacgtccg agacgtagcg ccagcccttc ttgttgcgtg cggtgtgcag
     9001 ccagtttgct gcgccgatga atgcgcgccg ctcgcgacct tgaatcgtga cgtcggtgcc
     9061 aatcgcggtg gctttcgcgc cgctgccgta ctcgagccgg acttcatcac cggcgcggta
     9121 cgtcgacgtc gggttgccga gcgcggcggc gtcggcgacg gctttgatcg ccacgtcatc
     9181 cggtgcgtcg tcgggaatcg ctgcgatgtg gatcgtcttc tcgtacgact tgtagctgac
     9241 ctcgccccag tcctcgatgt ggttctgggc gatgccgctt atcgcgtaga tgactgtgaa
     9301 gccgatcgcg agatacccga agtcgcggtg cacggcgcgc agccacgcgc gccagcgctt
     9361 cttgatccgc ggccacagct catccatacc gctcctcttt ccaaggatcg gcgtgattgt
     9421 gatagccgcg cacttcccag taaccgggcg cgtcgtcctt cacaaacttc acacccgtga
     9481 tccacttcgc gctcttccag aagtagagat cgggaatcag gccgcgcacg ggcgcgccgt
     9541 gcttgagctc gaacggcttg ccgttcaagc gatacgtgat cagcgcgttg tcggtcgtgg
     9601 cttccttgag cggcacgttg gccgtgtagc cgtgcgccgc ctcgaggatc acgtagcgcg
     9661 cctcgcccgt catctccgcg atctcggcga gcttcgcgat ctgcacgccc ttgtactcgc
     9721 caccgagcat cgtccagccg gtcacgcagt gaacgtccgc ggctttcgtg acctgaggca
     9781 gcttgagcaa gccggcgtag tcgacctcga acggcttctt cacgaggccg tgcaccttca
     9841 gcttgaacgt cttcacgtcg ccgtcgcctt cttggccacc catcgggcgc aagaactcga
     9901 tcacgcgctg cccgggaggt aggcgcgcac ggccgtcgcc gcgcttctcg gcgcgcgctt
     9961 gccgcgtcag gtcgtcagct gcgatacgga acagcgcgcc gccaaacgcc atcgcgatcg
    10021 ttccggctgc ggctgacttg atgaaattgc gacgatcgag cgccttcttc aactcgggaa
    10081 agcggtctgt gtccggggag tcgctcacgc tgcctccttc atcgtggcat acgagcaggg
    10141 cacgtattgg ttacgaacga cgccacgatc gggatcgccc aaaattcgcc gagttaccgg
    10201 gatgacgtat gcttagcgta atgggcagac ccagtcgatt cgacattgac tggcaacggc
    10261 tgggcagatg gatgctgctc ggcaccatcg tgatgttgtt gtggttactc gcgccggtcg
    10321 ccaagtgcag ctggtacgcg ttccgagaca ctccgatcgg cgaagtcaat gaagacgacc
    10381 tgccgggcca atccgacaag gagagggtcg agcagggcac cgggttcttt tcgcagtggg
    10441 gcaccgcgat caagggctgc tacgcgcaga cgccactgtt cggccaggag ccgtggaagg
    10501 ggaagctgct catcggcttc ggcgtcatga cggtgctcgc gcggacgatc ggctacatcg
    10561 aacggcgcaa caagcgcacg tacagctagc gtttcgcgac gtagatatcg agcgagccgt
    10621 tgcgcgtgct cgtgaagtag agcgtgtgtc cgtcgggaga cagccacgga tcgttttccc
    10681 cgtactgcgt gtcgatctcg aggaccagct gcggcgtctt gtacgaaccg tcgccgacgg
    10741 cttcggcacg ccagatgtcg tcgtctccct cggcgtcacc ggtgaaccag atcgcaccgg
    10801 tcgtatcggc gaacgcctcg ccttcgtagc ggttcgtatc gaggccggcg atcacgccgg
    10861 gcgtgcccca gctcgcaccc gccgacgctc gtgtcgagcg gaagatgtcg gagccgccgc
    10921 gcgcgggcga gcggtcggaa ccgaggaaga gcaccgtgtc gctccacggc tgcgcgttcc
    10981 agtcaccccc gctacccgag agcgagctga cgagcaccgg cgacgtccac gccgaagagc
    11041 gatccggccg cgtcgtgatg tagacgtcga cactcgtcga tggcggccgg ttgctcgaga
    11101 agtacatcgt gaggccgtcg gtcgagactt ccatcgacgt ctcgatccac tgcgtggaca
    11161 gctcgtcgat gcgtgccggc agcgaccacg cgtcgttcac gctcgcgcgg cgcgacgtgt
    11221 agatgtcgcc gttgccgagc gtgacgcgat ccgactcgaa gaagatctcg agcatgtcgc
    11281 cggtcagcgt gatgtcgtcg tcgcgatagt tcgcgctgga aatcggaacg agctcgggca
    11341 tgccgaaggg tccggttgga aacacgccag gcgtggcgtc gaccatgcgc ccatcgacgt
    11401 cgaccgcgtc gccagcgtcg ggcaattcac ccgatacaga agtgccgtgc tcgaagctac
    11461 agccggcgca cagccctaac gcgacccaga tccgcatccc tatgcatgac cgtacaccgg
    11521 cacgagtgtt cccgtcgtca acgcattgtc gctcgacgac aggaacgcga tcgtttgcgc
    11581 aatttgcgca ggctttggcc aactcgcgaa atcagcgttc ggcatcgccg cgcgattcgc
    11641 cggcgtgtcg atcaccgacg ggagcaccgc gttaactaaa atgttttcgg cgagcagctc
    11701 gacggcgagc gcttgcgtta tcgctgcgac gcccgctttc gatgcgacgt acgcgaccat
    11761 gccggccgtc ggcacgagca cggggcgcgc agcgacattg acgatgcggc caccggtgcc
    11821 ggccttgcgg atcgctttga ccgcttcgcg acacgtgagg aaacacgtcg cggtgttgag
    11881 ccgccactgc ttctcgaaat cggcgagcga cgtctcggcg atcggcgcca tcgcgaagcc
    11941 gccgacgagg tgaatcgacg cccacagatt cggcacgctc gcgtagaacg cggtgacctg
    12001 cgcttcgtca tcgagcgaca ccttcggaga cgcgtgcacg cgcgcgctgt cgcgccacgc
    12061 gaggtacttc ggcagctcgg gctcgaccat cggcacgtac acgtccgcgc cgcgctcgag
    12121 caatacagcg acgagaccgg cacccagtgc gccggtcgct cccgtcacga caatgttcat
    12181 cagagaatga tagcgccgca ggcagcctgg aacgtgtcca gagcctcctt caaacccgtc
    12241 gtcaggttgc catcacagat cgacgagaac acggcctgcg tgctcccgtt gccgttcgcg
    12301 agcgtgacga actgcttgag gcgcacgccg tccgccgcgc tgccgaagct cgagctgcag
    12361 ttgccgtcac cggcgatcac gccggctgcc caacgcgtac ggttgccctt gaggccgtcg
    12421 aggaacgaga cctcgtcctg cgggttccag ttcgtcggcg acatgccgct cgcgtcgagc
    12481 gtgaagttgt tgtccgtcgt cgacgcgtca tcttcgtcgg tgagcacgac gatcgcgagc
    12541 agtgcgtcgt cgcggaggaa gcccgcgttc gtatcgaccg cgcggtcctt gagcgcccac
    12601 ttcgccatga gcagcggcat ctcgatcgag gggccggtgg tgccgacgtt cgcgcggcac
    12661 gacagcgtcg acgccatgtt cgcgtccgag cgatcgagcc agcgcttggt gttgctgcag
    12721 ttgttctgga actcgccgtt ctggccctgc tcgctctgcg ggaacgttcc gaagccgccg
    12781 ccgagatcga tctcgtaatc gacatcgcga cccgtcgtcg tcaacgcgac gcgatagtcg
    12841 agcggcatgc cgttgttcac cgtgtactga tcgagcagct gcgcgaacat cgggaagttc
    12901 gacgcgaggt tcgattgctc ctcctgcatc gagcccgagt tgtcgacgat gaagacgatg
    12961 tccatcttgt cgcactgacg cgcttcgccc gagccactac cggatccaga accagatccc
    13021 gagcccgtgt cgtcgtcgtc accgtcacca cgcatcgtgg gaccgcacgc aaatagaagg
    13081 ctgctacacg caagaacaaa caatgcgttc ctcatcggaa tccttccctc cggttggccc
    13141 ctcagccacc cggcctcgat tgtgcgcgga ccatacacac gcctgatttc gatcggaagc
    13201 gaattcaggg cgcttcccgc ggtttgcgca agttgtgcaa gccgtccgtg gtgcgggctt
    13261 acgtttggga cgctacgcct cgaggccgac gtagatgtgc tggacgtcgt cgtcctcgtc
    13321 gagctcggcg aggaacttct cgacctcggc gcgtgctgct gcgtcgtcga gcttcaccgg
    13381 gttcttcgcg cgccagccga tccgcatcgt cgcgaccgtc cagccgcgcg tcgatagcgc
    13441 cttgctcacg gcgtcgacat cgttcggctc ggtgtagaag cgcacgccac cttcgccatc
    13501 cggctcgaag tcttgcgcgc ccgcttcgat cgcggcttct tcgctatcgg cttcgccact
    13561 cggcgtcgca tcgacaatgc cgacgtgcgt gaagtcccac gcgaccggtg tgagctggcc
    13621 gttgcgaaac acgtggcgca cgttcgtcat cgtgcgtttg ctgttgtcgg tgaggcactc
    13681 gacgatcacg gggacgcgat gcggcgcgaa accttcgacg acgacgttct cgtaattcgc
    13741 cttctcgtcg agcagcccgg cgcccttctt gatcgcgcgc tcgagcgtgt ccttgggcat
    13801 cgacgctttc ttcgcggctt cgaccgccgc gcgcagccga gcgttgagcg ccgggtcagg
    13861 gccgcctttc gcggcgacaa tgatttcctt cgccaagcgc gagaagacct tgcccttcgc
    13921 ggcggagctc gcgagccgac ctgcgtgttt ccattgggcg cccatggcgc ggtcttctat
    13981 acacgatcaa tccccggaga acgctatcga gcgctatcct cggggcggtg ccgcaccagg
    14041 tgacaggccg ggagccgacg gaggagttgg cgcacgaggg cgagcacacg caaatcgata
    14101 gcaccgagat cggcacgcag gccgaatcgc cgaaggcgct ctccgacgcc gcggcccagg
    14161 gctcgctgca cgcgaccgag cacgccccgg cgcgaccgtc gggctgggcg accacatcga
    14221 acaaccgcac gccgcacccg accgcggcgc aggcgttcga caagagcgac ctcgcgcgca
    14281 gcaacatgtt ccacatcttc ggcatctgtg cgccgctcgg cgcgctcgtg atgtcgctcc
    14341 tcatcggcgg cgatgagacc gcgcgctacg cgttttggat cggcgccggc atgatctcgc
    14401 tcggcaatgt cggcctcgtc tatttcacga gcaaaccgga gcgctacaac gcgacggtcg
    14461 tcggcctgct ctggatcttc gcgacgattg gcgtgcaacc ggcggtctat tacttcggcc
    14521 cgttctccgc cgtcgtgatg gtcgacatgc tcggcatcgt gttcatcgcg atcggccgcg
    14581 tgcgatggat ggcgcgcgcc accgcgatcg tttgcatcgg cggtcacctc gtgatcgcga
    14641 cgccgatcat cgcaggctgg attccagacg tcggcatcat ggcgagcatc gcggcaacga
    14701 acgagcagat gatcgttgcg gaggtgctca tcgtcgtgtt cctgacctcc gggtatttca
    14761 tcggtcggtg ggcgcgcagc acggcatcga gtgcgttccg cgagttgcag agcgcgatgc
    14821 ggatcatcgg cgatcgcgag caagcgctgc aggaagtcgt cgacgaagcc gcgcgccaga
    14881 agcgcgtcaa cgaaggccgg tggacgggcc agaagctcgg cacctggcgg ctcggcgttg
    14941 ttctcggtcg cggtgcgatg ggcgaggtct acgaagcagt gggcccgaag gatcgaccgg
    15001 ctgcgatcaa gctgctcaac gcgcgctcga cggcatcgac gtcgatcgtc gagcgctttc
    15061 atcgcgagat ggatgttgcg gcgcggctcg aatcaccgca catcgtgcat gtctacgagg
    15121 tgtcgacgcc cgacgcaccg gtgccgtaca tcgcgatgga gctgctccag ggcgtcgatc
    15181 tggcgacgaa gctgcgcaac gagaaccaga atcggatgcc gtccgacgag ctcgtcgtgc
    15241 tgatcgatca agtggcgcgc gggctcgagg tcgcgcggct cgcgggcgtc gtgcatcgcg
    15301 atctcaagcc gcacaacttg tttcatcacg acggctcggt ctggaagatc ctcgacttcg
    15361 gcgtctcgaa ggtgctcgac agcgaaggca cgctgaccgg cgaaggcatc gtcggcacgc
    15421 cgcaatacat ggcgcccgag caggcgtcgg gcggacaggt cacgcacctc gccgatgtct
    15481 acgcgctcgg cgcgatcgcg tatcgctgcc ttaccggccg ttcgccgttc aagggcaagg
    15541 acctcgcgga gctggtctac caggtcgtac atacgccacc cgcacgcccg agcacgctcg
    15601 gtcgcgtgtc gcagtcgatc gaggacgtgc tcgcggtcgc gatggcaaag gatccacggc
    15661 gccggtttac gtcggcaatg tcgttcgcgc aggcgttcat cgctgcacgg cgtggtcgca
    15721 aagtcgcgat cgacatcccc gacaacgcgt gggtgctgta gcagaacgcc gcgtagcaga
    15781 acgccacagc taaattcctg caacgcaacc cgagcggcag ttgtcacgat ttcgtgatca
    15841 gacgccacgt gctcgccgct ctcgcagccg gtgtcgaggc aggagatgac gaagccgcac
    15901 gcgaagtgct acgcggcacc gactgggtgc tccgcgcacc ggcgcagcgc gagctgtcgc
    15961 gcgcattgat cgatgcggcg ctgctgagcg gcgagttcgg cgcgagcgat cgcgagagcg
    16021 tgcgccacat ccatcgcgtc gctgcgctca acctcgcgaa gcgcttcgag gtcgacgtca
    16081 gcaatcgcga ggatgtcgcc gccgcatacg cgacgctgaa cgccgcacca ccggcgcgcg
    16141 caccgatcgc gacgatcttc gccggctcgg ccgtcgcgct tggtgtgctg ctgctcgtct
    16201 ggcttgcggt tgcgatccgc aggccgtcac atccggcgcg gcctattccg ccgcttgtca
    16261 ccggtgcgta cttctacggc ggtacgccgg cgcgcgacga agcgctcgag cagtatgtcg
    16321 tcaacgagct caccgatctc gtgatcgaga ccgacgccga gcgcggcggg agccacgacg
    16381 gtgcgccgcg cgcgaagcat gctgccgagc tgcgcaattc gtcggtgatc gcgtcgcgcg
    16441 gtccggctct ggcagcggcg tggcgcgcgt tgatcgacac gttcgatcac tgggtcgacg
    16501 tccgcgtgcg cggcaaggaa ttccgcagcg tcgaatccga gctcggccgt cgcgcgcagg
    16561 ccgtctccga tcagttcgct gcgctcgggc tcggcctcta tcttcagtcc gacgtgatgc
    16621 tggataacgg cgtcgcccac gcggcgatct tcgtgttcgc ggtcgaagaa gttgtctacg
    16681 tgcgtgctgg aggtgagccg cgccgcgtcc tgtcactacg gcgcctcgac gagctcaacc
    16741 tgcgccacac gttgctcggc cgccaaggtg acgagctcgg tgatccagtc gtgttgctcg
    16801 atcagatcga cgagttcgtg aatgcgcgcg tgctaccgac gctgcagggc gatgtctatc
    16861 cggtcggcga cgaggcgtgg cgctcgacga tctacggccg tggcatcgcg taccgcgccg
    16921 gcgaagcaat ccgaaccgag ctgctcgctg cggtcggcac cggatcgatc gaagagctcc
    16981 ggcccaaggt cgcgcgcatc atcgccgcga gcgtgcggcg tcacgaggcg cggcacggca
    17041 tcgacaacga gcggtcgact ccgctgcgct ttccgcgtca gctcgccgcg tacgtcgaca
    17101 acgacggcgg gccgatggca ttgcgcgcgc gtgccgagct cgccggctat ctcagtcaga
    17161 tcggcaacga gccggtgacg ccacagttcg cgctctggaa cctcgcgagt ctcacgttca
    17221 accgcacgcg ctggcagtcg gcggagtttt acgcgggcgt cgtcgtgatc gaagggctcg
    17281 cgcgcgagct tggcatcggc gatgggccca cggtgaagcg cgggcagttc gatcgccagc
    17341 gcctcgtcgc gctcgccgag ccgctcgccg cacagtcgag cgacaagctg cgcgccgccg
    17401 cacgcaatct gtggatcgag ctgtacggcg agccgatgct gccgatcatc gacgtcgcgc
    17461 gctagaactg ggtcgcggcg ccgaacgtca tcccgccgcc gggcatcggc gcgaccgagg
    17521 gcacgattgg cagtcggttc cagttgtcgt cgccgcgcgt acgcttcgcg aggtagaagc
    17581 tatcgatcat gatggggagg atcatggcgg tctcgccgag gaggatcgat cgcatcggca
    17641 ggcagacgaa cgactcggag ttattgcact cgtcctccgc cgaccggccg gcgaggtaga
    17701 cgccgccgac catcgcgacg cgcatcagca cgctgatcgt ggcggactcc gagttgccgt
    17761 acgcgacgtg aatcagtggt ggcagcagca gcgacggcgc tgcgaggtag tagcgatccg
    17821 gttgcaccat gaagtacgtc agcgggatca ccgcgagcaa atccaccggc agcgtccact
    17881 gcacgtagcg ctcgtcgacg agtggcgcgg tcgtcgggat tggcagatcg gcagctcgct
    17941 gcaccggtgg cgcgccgggc tgcggcgcga ctgggcgagg cggcggcaac ggccgcggcg
    18001 acgtgaccgt gacgcagccg ctcgcaagcg cgagggcgac gaacgcgcta acgaaaccgg
    18061 atcgcacggc cgtgccccct cagctcgttg tagctccacc agctcatgcc ctgcttgccg
    18121 aagtcgacga gcacgatcgc gtgcgcgccg atgcgtcctg cttgcgcacg cagcgcagcg
    18181 atcgcgtgtt ccttcgtcgg cgcctcgctg aacgcgttct tctggcgcac gaccacttcc
    18241 agatcgccga gcacgtcgta cggcacggcg acgtcggtct cgtacacttg cacggcggcc
    18301 ggatcggttt gatatggcac gcgcgcgttc gcgtatttca gatcgacatc gcgcgcgata
    18361 tcgaccgtgc acgccgcgag cgcgagcccg agcaccacga tcatcccccg catcattcga
    18421 tcgtaaccgg gtttcgcacg cgtccgacgg cggcgggcgg caatcgccag caacagacgg
    18481 ctggctgctt ccttgcctcg atttgctacg ctaatggtca tgagattgct catccgcgca
    18541 gtggtcactg gcttcggcct cgcactcggc tccgcgctgt tcaaagaagc gcgaacgcgc
    18601 ctgggttggg acgaagagga cgacgacagc aacgcgaaac gccaggagaa ggtgaaccag
    18661 caagacggcg cgacggaccc gggcttccaa cgtagttgac gctgcgtggc ggtgagaccg
    18721 ccaacactcc cgatccggaa cctgtcgaat tcagaagcgc attacgccgt tttgcggaac
    18781 actgagaggt agtggagtcc gccaatctgg cccccggctt attgctggcg atgccgcagc
    18841 ttgcggaccc gaacttctcg cgcgcggtcg tcctcatgat cgagcacagc gagcaaggct
    18901 ccttcggtct cgtgatcaat catccgagcc cgatcaaggc gagcgagctg ctcgatagcc
    18961 tcgaaatgag ctggcgcggc gaagactccg ccgtcgtgtg ggcaggtggc ccggtcagcc
    19021 cgagcacggg ctgggtgctg cacgagccgg tcggcggcac gcagcccggg acgatcgaga
    19081 tcacgtcgac gatctcgctc tcgacgtcgc cggatcgctt gcgcgcgatc gcgaatcaac
    19141 cgccacgcaa cgttcgcctg ttgctcggct attcgggctg gggccccggc cagctcgcgc
    19201 aggagatggc gcgcggtgcg tggctgcaca ccgcggccga tccgaggatc gtgttcgaga
    19261 cgccgcccga cgagatctgg gatcgcgcga tgaagtcgct cggcatcaac ccgcgcgact
    19321 tgttcaccgg ccgcggcgtg aactagtcgt agtactcggc gccgaggtgg gtgatcagct
    19381 cgtcgccctt cgtgtagcgc agcgtgttct tcagcttcat cagctgaatg aacagatcgt
    19441 gctcggggta gaggccctcg ggcgtcatcg gcgacttcca gtagaagctc agccactcct
    19501 ggatgcccga catgccggcg cggtgggcaa aatccatgaa cagcgcgagg tcgagcacga
    19561 tcggcgccgc gaggatcgaa tcgcggcaca ggaagttcac cttgatctgc atc
//
LOCUS       DASUFX010000073        15147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1957796, whole genome shotgun sequence.
ACCESSION   DASUFX010000073 DASUFX010000000
VERSION     DASUFX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15147
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957796"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV99_02760"
     CDS             <1..204
                     /locus_tag="VFV99_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058249.1"
                     /translation="ETASSIEIDGNAVAPSSKGGRFDVAPGHHRIRVKAPGRQPIDRE
                     FDVEPGGTAVLRVADDAGDAPSP"
     gene            complement(205..3270)
                     /locus_tag="VFV99_02765"
     CDS             complement(205..3270)
                     /locus_tag="VFV99_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5058250.1"
                     /translation="MLARSGFFCVVLFALVVGDAAADDHAAPTTLRMRPLRSRIFAKR
                     RLAQPTEPTAGERAAEGTEATPATPPADGTTPPADGAAAPPADQPNVAKTPDLTDEEL
                     AKMAEEQVGNETIVVTGSLIDRRELNTPAPVTVLDKADLDGAGVSTIGDIIQNLPAQS
                     NAINAQVNNGGDGSTRVDIRGLGANRTLVLLNGRRVVPGGTGADSSVDLNAIPLAVIE
                     RVEVLKDGASAIYGSDAIGGVVNIITRDDFNGTEVALYTGGSPRGDGFAYDMSFVTGH
                     SSKKGNIIFSAGWQHQSPVMAGDRTYSAFDKDYDFTEADPTMRESIGGSSATPGGRIA
                     PGAIDYDGDGIPDGMTNLCGAMVGACKPNGSGGWAPFTDDDLYNYQPSNYLFTPSQRY
                     NAFGKGHYDLSDNAKAFFEGLYLNRQSDQQLASEPFLAAVPISRDSIYNTTGGDVYDY
                     RRRLEEFGPRRSLQNVDTFRIVAGLSGKVPEDAPLLKNWKWELSYNYGHTTSTQEGDG
                     NLILSHLANAVGPSFIDTDGTPRCGTPAAPIPGCVPMNIMGPAGSINSQMRDYVTFTG
                     VSSGFNDQKTALAQAGGRITKLPNGGDVSLAVGTDYRQEAGGSTPDPLTATGDTTGNA
                     FAPTKGKYDVIEGFGELSVVPVSGLSAAQWLEFNLAARGFKYDTFGSGLTWKAGALFK
                     TVGGIAIRGTYSTAFRAPSVAELYQGKADDFPSATDPCDTDLDGDGVSDGPIADPVTA
                     RRCAQQGVAPNAVFGTSQQRAVVGGNADLDAETAKVFTAGLVIEPPAVKGLSFTLDYF
                     NIKISNAIQALGAQVILANCYTRDQDDACNQIHRDPQLAGAIDFVDDPVANVGGTTTD
                     GVDFALAYDHKQGGAGHFHHQFEGQILHKYTLDNTIQLLQGVGYYDLGVYPRLKANFS
                     TTWDLKPIDAGINVRYIGGYKECVDNDCNTPENLQMYSRNVDLNVTGDLFVGYTQKSK
                     VGTTRFTVGVNNITDQKPSLIYIGFAGDSDASTYDYMGRYVYARLSQLF"
     gene            complement(3338..3955)
                     /locus_tag="VFV99_02770"
     CDS             complement(3338..3955)
                     /locus_tag="VFV99_02770"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5058251.1"
                     /translation="MAETRSTSSKDRGDAILDAALDLAERGGFDHVRQREVAARANVA
                     LGTLYKRFRSKEDLLAAAMSREAERLEKKLERRPAHGSTIPTRVSAFFAIATRHICKR
                     PQVAHAMIRAMASGVPEIAAKVAGYQGRMTGLVIAAMRGRGKLEFVDAAGAPPTEREM
                     TYAFMLLQLWFASLVGWSAGLFVQTTVSDQMSRAAHVLAKGLDIG"
     gene            complement(3948..4877)
                     /locus_tag="VFV99_02775"
     CDS             complement(3948..4877)
                     /locus_tag="VFV99_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058252.1"
                     /translation="MMTTTRLALVSCLLAACASDNESELLAGFSPPPPTNGEVQYLSP
                     IVRDIKPGEDRIVCSYLDAYIDADSDIGRVAGYNTTGSHHIILYTTSLAQAPNTHDCK
                     DEEMVFFSIVGGTGGDAAESPENKLPEGLVRRVKGGNQLVIQTHWLNASDTAFDGQAA
                     FNVRYDSVSPNKTPADFLAVMNTSFDVTPGASKASVECTFEAGVNVWQLAGHQHSLGK
                     HVRIAYTPKAGEQRVLFDEDWKTEWSFNPKFLDFTLAPMYIAPGDKLRVDCDWENPGT
                     DTVRFPSEMCGAVGGFYPSTNQLICFNGDWLGG"
     gene            complement(4976..6271)
                     /locus_tag="VFV99_02780"
     CDS             complement(4976..6271)
                     /locus_tag="VFV99_02780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5058253.1"
                     /translation="MGADAQVAIVGAGFAGLAAARELAERGVSVVLIEATDRIGGRAR
                     TVRHVDSELPVELGPEFIHGCPDVTRTLAREAELAIEPIHERHYMHEGDRLVEVNDLW
                     EKLAAMLEGAPDASHDESARAFLERAKLPPRDARLFAMLVEGFYASPIDDIGIAGVAS
                     DAGGAGGASGDEPAQTRLSGGYGSLASWMRARLSTANTVVRHGCVVLAIEWQGSQVQL
                     ACSEYGRDTVVVADRAIVTLPLGVLQAGAVRFTPALGDHAMALSRLAMGQVVKIVLCL
                     REPVWHELGPHDLAFAHDTDTAFPSFWMRTRDHATTLTAWAGGPHARALASYSADALA
                     ERAIDEFALTVSLPRGRVAAAVQHYHFHDYGKDPFARGAYSYTRVGGMGVAAQLSRPL
                     GDRLVLAGEATDEEYEGSVAGALASGQRAAKQVLAMIGR"
     gene            complement(6299..7975)
                     /locus_tag="VFV99_02785"
     CDS             complement(6299..7975)
                     /locus_tag="VFV99_02785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058254.1"
                     /translation="MGKYADEFKAGDRLGKYEIIRELATGGMATIYLARVSGTAGFEK
                     LVVLKCILPSLARDRSFVNMFLDEARLAATLRHSNIADVFDVGVEDNTFFFAMEYIHG
                     QNARTVRIEAKQRQMPIPLEVSLAIVVGTASALGYAHDRTSADGPLHLVHRDVSPSNV
                     IVSYEGAVKLVDFGIARANLRRGAKTRTGFKRGKAPYMSPEQCRGEAIDNRSDLFSLG
                     TMLYELTVGQRPFLGNSDFEVMESIVVNDPAPPSILVRGYPARLEKIVMRLLARSPGA
                     RYQRANLVVDELESFIAERSMLTSSYVVAKYLGELFSELATSKFERTITNSSLVTPDT
                     REYPAGRPPTALMETLGSKHHHADDEPTAAFKRAEQLWTPELRVRVRTPGEEPSQTSA
                     FHEPTRQDPVLPFDPIAARSDELLAHLDDDAPAGEKPQARAYRRVETLLHRALAYAGM
                     DEMDKAVTAIELALDEEPRTDDVRELLQSHVETIVGVYEGLLEDPYRTPTLVRPMHEL
                     IPIEIEPRARLLLPWIDGTTSIRDLIARSGMQRLEAYHHLCQFLLRGIIN"
     gene            complement(8005..8778)
                     /locus_tag="VFV99_02790"
     CDS             complement(8005..8778)
                     /locus_tag="VFV99_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058255.1"
                     /translation="MAAPPTEAYSGGAAAYDPAPPSSYQPAHAAESGSSIAQAQPVPD
                     RPGLGTTYGEQVYAPISFAPFVRSSAQAWAEVLLHYNDADGIEAHAQYLGTRPQPLEI
                     YAGDGSLAVALVDESGHTLPGLQAQGRTLIVGEDGARYKIIVRNGTTARFEVVASVDG
                     LDVIDGKPADPNRRGYIVDPHDTLVIDGFRTSDANVAAFRFGKVADSYAARTSGDRNV
                     GVVGLAIFSERGAVWTPAELQTRDTANPFPSRGYAMPPR"
     gene            complement(8899..9549)
                     /locus_tag="VFV99_02795"
     CDS             complement(8899..9549)
                     /locus_tag="VFV99_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058256.1"
                     /translation="MTKLAALLLVASTASAQAQTYVATDGGPRDRTPREVDGRLGMLL
                     GGSDVGDADGFSMGISGALGYRMGDLTLRGLVDYYRVGDNADELMDRKGRATRLGGAL
                     RYSFANTGFESKFAADFWGELGAGYEHVAWRNGGVLDRPSGELAFGLDIGRRGAPNAR
                     GDRKRIGYFMAFRSLVAQGPEMAGATATCSGPCSEATKPPRTDVTMFFELGVHWGR"
     gene            complement(9661..10008)
                     /locus_tag="VFV99_02800"
     CDS             complement(9661..10008)
                     /locus_tag="VFV99_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058257.1"
                     /translation="MPRWQRAYVIAMCAIIGAAFAYAACDWGQWPKLVYAPLSGELMT
                     KAPANAITIVYLGVVAWGLGGAAVGAIIGGALCAITKTKPWPARALQLFGAWAITGIV
                     LAGSYYTWSLWPW"
     gene            complement(10008..11480)
                     /locus_tag="VFV99_02805"
     CDS             complement(10008..11480)
                     /locus_tag="VFV99_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5058258.1"
                     /translation="MRLAVAALVVIATLGLTGFDHIVHDRNLDVKGVGVLPCNKCHVE
                     TKGKLVGKPGHAACFGACHGAAPTAPKRGAKLVLGDNAQVCTSCHADAVLQKPYAGKL
                     PVFYPPYTADPDFNISFGHKQHSAAACTQCHDIRAKAKPATHARCTNCHDGSSAQGHT
                     TAMANCTTCHPRAVGKPQPPELALVRDSVTAVFSHTKHAGRSAAGRDCNTCHAAIRAT
                     DDTELPRPKQDDCATSGCHDGKAAFSTTSACTRCHDKAPDRFEVNRPQKRFLHTGVHA
                     DAVKGRPCAACHPLSPRGEVEIIGHAACTGCHAKDFGERSPLICGACHNATEPWRKLV
                     ADRALPERTEFGAMLDHDKHKGACASCHKLRTASAQLRTPRGHASCIGKGCHENKRGP
                     APTFESCDGCHRLGLAAERESTRLAAAWSVRRAFDHASHVETPDHQPLACVACHPVLS
                     GSVLELATPKKPACLPCHDDGKAAFKLTGTTCTRCHTGGR"
     gene            complement(11480..13237)
                     /locus_tag="VFV99_02810"
     CDS             complement(11480..13237)
                     /locus_tag="VFV99_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058259.1"
                     /translation="MTRAFAFVLALVLVPTATARAEPAKLTLTHEPPPRNLKMRVRTA
                     AASKPDPAPASALTPAPAEDASVKAAAREPAVELGSVRDLRRPVSVRFNMGYVVDGTA
                     LTGKDNLAGKPLEPYEFSQLHAYALGEGYFSSRGVMLQSLSTYFASRFQLTRSTMSFD
                     PMAPTPKDVRVAPPIANWFDRSGFDLREGWGEVQDFLPDKRLAPLRLRAGSLYVYGPW
                     MLHMVGAVAAWEGKLIRGSLYSGFRVADYTRVDRPDGKNRAGIGGSSLRIDFRALKTP
                     IPFAISGEFLAFTAVGAAADKASQHSQIQIDWRPRKDIAVIGSARTLDGVLANEHVQL
                     RSRYKQVTNLVFDVTHRRDTDWRWDPSVPQNDPLETKRYLDLGPVVPQLLVSGRAGTL
                     IAENIDLLLRGAYANDLVSNNIARNTFAASYFEVGGGLEVRLRRTIGLGLTGVTRQTS
                     RQATLAEQIPDVPLVADEMPRQFGPELGEESFTELGTTVRMSLGARRFSAQVEVYGRR
                     TRYAEVYCDAAAVADCRSDIDTGIQTSEYRGGGRATIDAWIGSRLRLLASYELSSRLD
                     LQPEIYGFKSLRLMIEGVY"
     gene            complement(13234..14457)
                     /locus_tag="VFV99_02815"
     CDS             complement(13234..14457)
                     /locus_tag="VFV99_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5058260.1"
                     /translation="MARSFLIAVLVLIGCSSARTIEAPAPPIDKTRLSHSQHAQIKCE
                     TCHAMDKRPGSDDHKPCDGCHKQAFLSAPGELCKVCHTQVTATPLAAPLRPYPIEDIW
                     RAEPVRFSHAIHMDFSRMEGRVGFHVACADCHVRDGSLATPNHATCARCHAPETHLDN
                     APAMTSCNGCHKNGVNLRTRARLIKNDLRFDHEHHKSDRRGTAIRCEECHAQSAASTT
                     YTDHAPPRIERCVGCHDDTQRTPAELRMRACETCHSQRTQTLTSLAPRNHLPATERPL
                     DHTLAFRRDHAEAAERDGARCGACHTQMSGNGRDNCDECHQTMRPSDHRITFRELDHG
                     AEAAADRDRCARCHVIEFCTACHAQRPRSHGFPGMFANGDEHGGIARVNVRSCLTCHD
                     QQTTCMGSGCHTVRP"
     gene            14590..14745
                     /locus_tag="VFV99_02820"
     CDS             14590..14745
                     /locus_tag="VFV99_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058261.1"
                     /translation="MRTSEVDPNASGEIEIPPPAKPQRVVDKKTVVVDKRGLLPIRKP
                     RALTIKE"
     gene            complement(14735..>15147)
                     /locus_tag="VFV99_02825"
     CDS             complement(14735..>15147)
                     /locus_tag="VFV99_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058262.1"
                     /translation="GIAPELRGSCDLVTCNAPIPEPGGGDPYRARWRMTDATFIERMF
                     VHARGFVAPDGMVVVHAALDALEPVLAELHGHRVLIAYTPEGVRGFAVAWWRPDGEDR
                     LVRGRRLLTDWQPHLTHEDRIAALTRTLPPLTTP"
BASE COUNT         2437 a         4906 c         5164 g         2640 t
ORIGIN      
        1 gagaccgcat cgtcgatcga gatcgatggc aacgcggtgg caccgtcgag caagggcgga
       61 cggttcgacg tcgcgcccgg tcatcaccgg attcgcgtca aggcaccggg gcgccagccc
      121 atcgatcgcg agttcgacgt cgagccgggt ggcaccgcgg tgctgcgcgt cgccgacgat
      181 gcgggcgacg cgccgtcgcc ctgatcagaa cagctgcgac aggcgcgcgt agacgtaacg
      241 acccatgtag tcgtaggtcg acgcgtccga gtcgccggca aagccgatgt agatgagtga
      301 cggcttctgg tcggtgatgt tgttgacgcc gaccgtgaag cgggtcgtgc ccaccttcga
      361 cttttgcgtg tagcccacga acaggtcgcc cgtgacgttg aggtcgacgt tgcgcgagta
      421 catctggaga ttctcgggcg tgttgcagtc gttatccacg cactccttgt agccgccgat
      481 gtagcgcacg ttgatgccag catcgatcgg cttgaggtcc cacgtcgtcg agaagttcgc
      541 cttgagccgc ggatacacgc cgaggtcgta gtagccgacg ccctggagca actggatcgt
      601 gttgtcgagc gtgtacttgt ggaggatctg gccctcgaac tgatggtgga agtggcctgc
      661 gccgccctgc ttgtggtcgt aggcgagcgc gaagtcgacg ccgtccgtcg tcgtaccacc
      721 gacgttcgcc accggatcat cgacgaaatc gatggcgccg gcgagctgcg gatcgcgatg
      781 gatctggttg catgcgtcgt cctgatcgcg tgtgtagcag ttcgcgagga tcacctgcgc
      841 gccgagcgcc tggatcgcat tcgagatctt gatgttgaag tagtcgagcg tgaacgacag
      901 gcccttcacg gccggtggct cgatcacgag accagcggtg aacacctttg ccgtctcggc
      961 atcgagatcc gcgttgccgc cgacgacggc acgctgctgc gaggtgccga acacggcgtt
     1021 tggcgcgacg ccttgctgcg cgcaacgacg cgcggtgacc ggatccgcaa tcggaccatc
     1081 gctgacgccg tcgccgtcga ggtcggtgtc acacggatcg gtcgcgctcg ggaagtcgtc
     1141 ggccttgccc tggtagagct cggcgaccga cggcgcgcgg aacgcggtcg agtacgtgcc
     1201 gcggatcgcg ataccgccga ccgtcttgaa cagtgcgccg gccttccagg tcaagccgct
     1261 gccgaacgtg tcgtacttga agccgcgtgc ggcgaggttg aattcgagcc attgcgcagc
     1321 ggacagaccc gacacgggca ccaccgacag ctcgccgaag ccttcgatca cgtcgtactt
     1381 gcccttcgtc ggcgcgaacg cgttgccggt cgtgtcgccg gttgcagtca gtgggtccgg
     1441 cgtcgaaccg ccggcttcct ggcgatagtc cgtaccgacg gcgagcgaga cgtcaccgcc
     1501 gttcggcagc ttcgtgatgc ggccacccgc ctgcgcgagc gccgttttct gatcgttgaa
     1561 gccgctgctg acgccggtga acgtgacgta gtcacgcatc tggctgttga tcgagcccgc
     1621 cgggcccatg atgttcatcg gcacgcagcc ggggatcggg gcagccggtg ttccgcaacg
     1681 cggcgtgccg tcggtatcga tgaagctcgg cccgaccgcg ttcgcgaggt ggctcaggat
     1741 caagttgcca tcgccctcct gcgtcgacgt ggtgtggccg tagttgtacg agagctccca
     1801 cttccagttc ttgagcagtg gcgcgtcctc gggaaccttg cccgacaggc cggcgacgat
     1861 gcggaacgtg tcgacgttct gcagcgagcg gcgcggaccg aactcttcga gccggcggcg
     1921 gtagtcgtac acgtcaccgc ccgtggtgtt gtagatgctg tcgcgcgaga tcgggaccgc
     1981 cgcaaggaac ggctcggacg cgagctgctg atcggattgg cggttgaggt agaggccctc
     2041 gaagaacgcc ttcgcgttat cgctgaggtc gtagtgaccc ttgccgaacg cgttgtagcg
     2101 ctgcgacggc gtgaacaagt agttcgacgg ctggtagttg tagaggtcgt cgtcggtaaa
     2161 cggtgcccag ccaccgctac cgtttggctt gcatgcgccg accatcgcgc cgcacaggtt
     2221 tgtcatgccg tcgggtatgc cgtcgccgtc gtagtcgatc gcaccggggg cgatgcggcc
     2281 gcccggcgtc gcgctcgatc cgccgatgct ctcgcgcatg gtcggatcgg cttcggtgaa
     2341 gtcgtagtcc ttgtcgaacg ccgagtacgt gcgatcgccg gccatcaccg ggctctggtg
     2401 ctgccagccc gccgagaaga tgatgttgcc cttcttcgat gagtgaccgg tgacgaagct
     2461 catgtcgtac gcgaagccgt cgccgcgcgg cgagccgccg gtgtagagcg cgacctcggt
     2521 gccgttgaag tcgtcacggg tgatgatgtt gacgacgcca ccgatcgcat cggagccgta
     2581 gatcgcggag gcgccgtcct tgaggacttc gacgcgctcg atcacggcga gcgggatcgc
     2641 gttgagatcg actgacgagt cagcgccggt accgccgggc acgacacggc ggccgttgag
     2701 gagcacgagg gtgcggttcg caccgaggcc acggatgtcg acgcgcgtcg agccgtcacc
     2761 gccgttgttg acctgcgcgt tgatcgcgtt cgactgcgcg ggcagattct ggatgatgtc
     2821 gccgatcgtc gacacgccgg cgccgtcgag gtcagccttg tcgagcacgg tgaccggggc
     2881 aggggtgttg agctcgcgac gatcaatgag cgagccggtc accacgatcg tttcgttgcc
     2941 gacttgctcc tcggccatct tggcgagctc ctcgtcggtc aggtccggtg tcttggcgac
     3001 gttcggctgg tccgcgggtg gcgcagccgc accatcggcg ggcggcgtcg tgccgtcggc
     3061 cgggggcgtg gccggggtcg cctcggtacc ttcggcggcg cgttcgccag ccgtcggttc
     3121 cgtcggctga gcgagccggc gcttcgcgaa gatccgtgaa cgcagcggac gcatccgcaa
     3181 cgtcgtcgga gcagcatggt cgtcagcggc agcgtcgccg acgacgagag cgaacagaac
     3241 aacgcagaag aatccacttc gtgccaacat gtcgcgggac cgtcgcacgg tgtcgcggta
     3301 gtccccaatg ctacatcggt aacggccgct gacttgatca gccgatgtcg agacctttgg
     3361 ctaacacgtg cgccgcacgc gacatctgat cgctgacggt cgtttgcaca aatagtcccg
     3421 ccgaccagcc gaccaatgat gcgaaccaaa gctgaagcaa catgaaggcg taggtcatct
     3481 cacgctcggt tggcggtgcg cccgcagcgt cgacgaactc gagcttgccg cgaccccgca
     3541 tcgcagcgat gacaaggccg gtcattcgtc cctgataccc ggcgaccttt gcggcgatct
     3601 cgggcacgcc ggacgccatg gcccggatca ttgcgtgcgc gacttgcggc cgtttgcaga
     3661 tatggcgcgt cgcgatcgca aaaaatgcgc tgacccgcgt ggggatcgtg ctgccgtgcg
     3721 cggggcggcg ctcgagcttc ttctccagtc gctcggcttc gcggctcatc gcggctgcga
     3781 ggagatcttc tttactacgg aaccgcttgt agagcgtgcc gagtgcgacg ttcgcgcgag
     3841 cggcgacttc gcgctggcgc acgtggtcga agccgccgcg ttcggcgagg tcgagcgcag
     3901 catcgaggat cgcgtcgccg cggtctttgg aagaagtaga acgtgtttca gccacccaac
     3961 cagtctccgt tgaaacagat tagctgattc gtcgaaggat agaagccacc aactgcacca
     4021 cacatctcgc tcgggaacct cacggtgtcg gtgccggggt tttcccagtc gcaatcgacg
     4081 cgcaacttgt cgccaggtgc gatgtacatc ggcgcgagcg tgaaatcgag gaacttcgga
     4141 ttgaacgacc actcggtctt ccagtcctcg tcgaagagca cgcgttgttc gccggccttc
     4201 ggcgtgtacg cgatgcgcac gtgcttgccg agcgaatgct gatgaccggc cagctgccac
     4261 acgttcacgc cggcttcgaa cgtgcactcg acgctcgcct tcgatgcacc aggcgtgacg
     4321 tcgaacgacg tgttcatcac ggccaagaag tcggccggcg tcttgttcgg cgacacggag
     4381 tcgtaacgca cgttgaatgc ggcctgcccg tcgaacgcgg tgtcgcttgc attgagccag
     4441 tgcgtctgga tcacgagctg gttgccgccc ttcacacggc gaacgagccc ctcgggcagc
     4501 ttgttctcgg gtgactcggc ggcatcgccg ccggtgccgc cgacgatact gaagaacacc
     4561 atctcttcgt ctttgcagtc gtgggtgttc ggcgcctgcg cgagcgaggt cgtgtacagg
     4621 atgatgtggt ggctgccggt cgtgttgtag ccggcgacgc gcccgatatc ggagtccgcg
     4681 tcgatgtatg cgtcgaggta actgcacacg atgcggtcct cgcccggctt gatgtcacgc
     4741 acgatcggcg acaggtactg cacctcgccg ttcgttggcg gcggcggcga gaagcccgcg
     4801 agcagctcgg attcgttgtc actcgcgcaa gccgcgagca ggcacgacac aagagcaagg
     4861 cgcgtggtgg tcatcatcgg ggcggactag aacatgttct acttttcacc gacaacgcag
     4921 ctgcgcagca aatgcgcctc agttgccgct gtgtctgcca attacgtaag agccgttagc
     4981 ggccgatcat cgcgagcacc tgcttggcgg ctcgttggcc gctcgcgagc gctcccgcga
     5041 ccgagccttc gtactcttca tcggtcgcct cgccggcgag cacgagccga tcaccaagcg
     5101 gcctgctcag ctgcgccgcg acgcccatgc caccgacgcg cgtgtagctg tacgcgccgc
     5161 gtgcgaacgg atccttgccg tagtcgtgaa agtgatagtg ctggacggca gctgcgaccc
     5221 gaccgcgggg caggctgacc gtcagtgcga actcgtcgat cgcgcgttcg gcgagtgcgt
     5281 ctgccgaata gctcgcgaga gcacgtgcat gcgggcctcc tgcccaggcg gtgagcgtag
     5341 tcgcgtggtc gcgcgtgcgc atccagaacg acgggaatgc ggtgtcggta tcgtgtgcga
     5401 atgccaagtc gtgcggcccg agctcgtgcc acaccggttc gcgcaggcag aggacgatct
     5461 tcacgacctg acccatcgcg agtcgcgaga gcgccatcgc gtggtcgccg agcgcaggtg
     5521 tgaagcgcac ggcacccgct tgcaggacgc cgagtggcag cgtgacgatc gcgcgatccg
     5581 caacgacgac ggtgtcgcgg ccgtattcgc tgcacgcgag ctggacctgg ctgccttgcc
     5641 actcgatcgc gaggacgaca cagccatggc gcacgacggt gttcgcggtg gacaggcgcg
     5701 cgcgcatcca gctcgcgagt gagccgtaac cgccggataa ccgcgtttgc gccggttcgt
     5761 ctccgctcgc gcctccggcg ccgccggcat ccgatgcgac gcccgcgatg ccgatgtcgt
     5821 cgatcggcga cgcgtagaag ccttcgacca gcatcgcaaa caagcgcgca tcgcgcgggg
     5881 gcagcttcgc gcgctcgaga aacgcgcgtg cggactcgtc gtggcttgcg tcgggcgcgc
     5941 cctcgagcat cgccgcgagc ttctcccaga gatcgttgac ctcgacgagg cgatcgccct
     6001 cgtgcatgta gtggcgctcg tgaatcggct cgatcgcgag ctccgcctcg cgtgcgaggg
     6061 tacgcgtcac gtcgggacag ccatggatga actcggggcc gagctccaca ggaagctcgg
     6121 agtcgacatg ccgcacagtg cgcgcacggc caccgattcg atcagtggct tcgatcagca
     6181 cgaccgagac gccgcgctcg gcaagctcgc gtgcggcagc aagtcccgcg aagccagctc
     6241 cgacgattgc gacctgggcg tccgctccca tgtgcggatc aggtgcaagc cgtacgcctt
     6301 agttgatgat gccgcgcaag agaaactggc agaggtggtg atacgcctcg agccgttgca
     6361 ttccggatcg cgcgatcaaa tctctgatcg acgtggtgcc atcgatccac ggcaacaaca
     6421 ggcgcgcacg tggctcgatc tcgatgggaa tgagctcgtg catcggccgc acgagcgtcg
     6481 gcgtgcggta cggatcttcg agcaggcctt cgtagacgcc gacgatcgtc tcgacgtggc
     6541 tctgcaacag ctcgcgcacg tcgtcggtgc gtggctcctc gtcgagggcg agctcgatcg
     6601 cggtgacagc tttgtccatc tcgtccatcc ccgcatacgc cagtgcacga tggagcaggg
     6661 tctcgacgcg ccgatacgca cgcgcctgtg gcttctcgcc ggcaggcgcg tcgtcgtcga
     6721 gatgagcgag cagctcgtcg ctgcgcgccg cgatcggatc gaacggtagt accgggtcct
     6781 ggcgtgtggg ctcgtggaat gccgaggtct ggctcggttc ctcacccggc gtgcggaccc
     6841 gcactctgag ctccggcgtc cacagttgct cggcacgttt gaacgcagcc gttggctcgt
     6901 catcggcgtg gtggtgtttg ctgccgagcg tctccatcag cgcggtgggc ggccggccgg
     6961 ccggatactc gcgggtgtcg ggcgtgacca gcgacgaatt ggtgatcgtg cgttcgaact
     7021 tcgaggttgc gagctcgctg aacagctcgc cgagatactt cgcgacgacg tacgacgacg
     7081 tcagcatgct gcgctcggcg atgaaactct cgagctcgtc gaccaccaag ttcgcgcgct
     7141 ggtagcgcgc acccggcgag cgagcgagca agcgcatcac gatcttctcc agccgggctg
     7201 gatagccgcg gacgaggatc gacggtggtg ccggatcgtt caccacgatc gactccatga
     7261 cctcgaagtc gctgttgccg aggaatgggc gctggccgac cgtgagctcg tagagcatcg
     7321 tgccgagcga gaacagatcg ctgcggttgt cgatcgcctc gccgcggcac tgttcgggcg
     7381 acatgtacgg cgctttgccg cgcttgaatc cggtgcgtgt cttcgcgcca cggcgcaggt
     7441 tcgcgcgcgc gatgccgaag tccacgagct tgaccgcgcc ttcatagctg acgatcacgt
     7501 tgctcggcga tacatcgcgg tgcacgaggt ggagcggtcc gtcggcgctc gtgcggtcgt
     7561 gcgcgtagcc aagcgccgag gcggtgccga ccacgatcgc gagcgagacc tcgagtggaa
     7621 tcggcatctg gcgctgcttc gcttcgatgc gaacggtgcg cgcgttctga ccgtggatgt
     7681 actccatcgc gaagaagaac gtgttgtctt cgacgccgac gtcgaacaca tctgcgatgt
     7741 tcgaatgccg cagcgtcgcc gcgagccgcg cttcatcgag gaacatgttc acgaagctgc
     7801 ggtcgcgcgc gaggctgggc aggatgcact tcaagacgac gagcttttcg aacccggccg
     7861 tacccgacac acgtgcgaga tagatcgtcg ccatcccgcc ggtcgcgagc tcgcgaatga
     7921 tctcgtactt gccgagtcga tcgcccgcct tgaattcgtc ggcgtacttg cccactccga
     7981 cgacacgcta acacgcggag cgcgtcagcg cgggggcatt gcgtagccgc gcgacgggaa
     8041 cggattggcc gtatcccggg tctggagctc ggccggcgtc cacaccgcgc cacgctcgga
     8101 gaagatcgca aggccgacga caccgacatt gcggtcgcca gaggtacgcg ccgcgtaaga
     8161 atcggcgacc ttgccaaaac ggaatgctgc gacgttcgca tccgacgtgc ggaagccgtc
     8221 gatcacgagc gtgtcgtgcg ggtcgacgat gtagccgcgg cgattcgggt ccgctggctt
     8281 gccgtcgatc acatcgaggc catcgacgga ggcgacgacc tcgaagcgcg ccgtcgtccc
     8341 attgcgcacg atgatcttgt agcgcgcgcc gtcctcaccg acgatcaatg tgcggccctg
     8401 ggcctggagg ccgggcaggg tatgccccga ttcgtcgacg agcgcgaccg cgagcgagcc
     8461 atcgccggcg tagatctcga gcggctgagg ccgcgtaccg agatattgcg cgtgcgcctc
     8521 gatgccatcc gcgtcgttgt agtggagcag cacttccgcc catgcctgcg ccgacgagcg
     8581 cacgaacggc gcgaagctga tcggagcgta gacctgctcg ccatacgtcg tgcccaggcc
     8641 cgggcgatcg ggcaccggct gtgcttgcgc gatcgacgat ccggactccg cggcgtgcgc
     8701 cggctgatac gacgacggcg gcgccgggtc gtatgccgcg gcgccgcccg agtacgcctc
     8761 ggtcggcggc gctgccatct ttggggagcc gccgtacatc gagccgccgc acgcggcgac
     8821 gagcgcaaga caaaccggta gcgatgagtg ccgcagcact ctggtggagc tcaacatgat
     8881 ctttcctttc gggttcgctc agcggcccca gtgcacgccg agctcgaaga acatcgtcac
     8941 gtcggtgcgc ggcggcttcg tcgcctccga gcacggacca ctgcacgtcg ccgtcgcgcc
     9001 ggccatctcc ggtccctgcg cgacgaggct gcggaacgcc atgaagtaac caatccgttt
     9061 gcggtcaccg cgcgcgttcg gtgcgccgcg gcggccgatg tcgagaccga acgcgagctc
     9121 accgctcggc cgatcgagga cgccgccgtt acgccacgcc acgtgctcgt aaccggcgcc
     9181 gagctcgccc cagaagtcgg ccgcgaactt cgactcgaag ccggtgttcg cgaacgagta
     9241 gcgcagcgcg ccgccgagtc gcgtcgcgcg acccttgcga tccatcagct cgtccgcgtt
     9301 gtcaccgacg cggtagtaat cgacgaggcc gcgtagcgtc aggtcgccca tgcgatagcc
     9361 gagcgcgccc gagatgccca tcgagaagcc gtcggcatcg ccaacgtccg agccgccgag
     9421 caacatgccg agccggccgt cgacctcacg cggcgttcga tctcgcggcc caccgtcggt
     9481 ggcgacgtac gtctgcgcct gcgccgaggc agtcgaagca accagaagca gcgcggctag
     9541 tttcgtcata cgccaaccca acgcaccttg cggccgtgac ttacagcgcg actgtttccg
     9601 cgctgtccca cactgtatgg ccatgcttcg cctggtcttg atctccgcgg gtttgctcga
     9661 tcaccacggc cacaagctcc acgtgtagta gctacccgcg agcacgatgc cggtgatcgc
     9721 ccacgcaccg aacagctgca gcgcgcgcgc cggccacggt ttggtcttcg tgatcgcgca
     9781 cagcgcgcca ccgatgatcg cgccgaccgc ggcgccgccg aggccccacg cgacgactcc
     9841 gagataaacg atcgtgatcg cgttcgctgg cgccttggtc atcagctcgc cgctcagcgg
     9901 tgcatagacg agcttgggcc actgtcccca gtcgcacgcg gcgtacgcga atgcagcgcc
     9961 gatgatcgcg cacatcgcga tgacataggc gcgttgccag cgcggcatca cctgcctccc
    10021 gtgtggcagc gcgtgcacgt cgtgccggtg agcttgaatg cggccttgcc gtcgtcgtgg
    10081 cagggcaggc atgccggttt cttcggcgtc gcgagctcga gcaccgaacc gctgagcacc
    10141 gggtgacacg cgacgcacgc gagcggctga tgatcgggtg tctcgacgtg cgatgcgtga
    10201 tcgaacgcgc ggcgcaccga ccacgcggca gcgaggcgcg tgctctcgcg ttccgcggcg
    10261 aggccgaggc gatgacagcc atcgcagctc tcgaacgtcg gcgccggccc acgcttgttc
    10321 tcgtgacagc ccttgccgat acacgatgcg tgaccgcgtg gcgtacgcag ctgcgccgat
    10381 gccgtgcgga gcttgtggca gctggcgcaa gcgcctttat gcttgtcgtg atcgagcatc
    10441 gcaccgaact cggtgcgctc gggcagcgcg cgatcagcga cgagcttgcg ccacggttcg
    10501 gtcgcgttgt ggcacgcgcc gcagatcagt ggtgagcgct caccgaagtc cttcgcgtgg
    10561 catcccgtgc acgcggcgtg accgatgatc tccacttcgc cacgcgggct cagtggatga
    10621 catgccgcgc acggccggcc cttgaccgcg tcggcgtgga caccggtgtg caggaagcgc
    10681 ttctgcgggc ggttgacctc gaaacgatcg ggtgccttgt cgtggcaccg cgtgcatgcg
    10741 gacgtcgtgc tgaacgcggc cttgccgtcg tggcagccgc tcgtcgcgca gtcgtcctgt
    10801 ttcgggcgtg gcagctcggt gtcgtcggtg gcgcggatcg cagcgtggca cgtgttgcag
    10861 tcgcggccag cggcgctccg tcccgcgtgc ttggtgtgcg agaacaccgc cgtcaccgag
    10921 tcacgcacga gcgcgagctc cggtggctgc ggcttaccaa ctgcgcgcgg atggcaggtc
    10981 gtgcagttgg ccatcgccgt cgtgtgtccc tgcgcgctcg agccatcgtg acaattggtg
    11041 cagcgcgcgt gcgtggccgg cttggccttc gcgcggatgt cgtgacactg cgtgcatgca
    11101 gcggcgctgt gctgcttgtg accgaacgag atattgaagt ccggatccgc ggtgtacggc
    11161 ggatagaaca ccggtagctt gccggcgtac ggcttctgca gcactgcatc ggcgtggcag
    11221 ctcgtgcaca cctgcgcgtt gtcaccgagc acgagcttcg cgccgcgctt gggcgccgtc
    11281 ggcgcagcgc catggcatgc gccgaagcag gctgcgtgtc ccggtttgcc gacgagcttg
    11341 cccttcgtct cgacgtggca cttgttgcat ggcaacacgc cgacgccttt cacgtcgagg
    11401 ttgcggtcgt gcacgatgtg atcgaagccg gtgagcccga gcgtcgcgat cacgacgagc
    11461 gcggcgacgg cgaggcgcat cagtacacgc cctcgatcat gagtcgcagg ctcttgaagc
    11521 cgtagatctc gggctggaga tcgagacggc tcgacagctc gtagctcgcg agcaaccgca
    11581 gccgcgagcc gatccacgca tcgatcgtcg cgcgacctcc gccgcggtac tcgctcgttt
    11641 gaatgccggt gtcgatgtcg cttcgacaat ccgcgacggc cgcggcgtcg caatacacct
    11701 ccgcgtagcg cgtgcgccgg ccgtacactt cgacctgcgc cgagaaccgg cgcgcgccga
    11761 gtgacatccg gaccgtggtg ccgagctcgg tgaaactttc ctcgccgagc tcggggccga
    11821 actgccgcgg catctcgtcg gccacgagtg gcacgtccgg aatctgctct gcgagcgtcg
    11881 cctgtcggct cgtctgtcgc gtgacgccgg tgagcccaag gccgatcgtg cggcgcaacc
    11941 gcacctcgag accgccgccg acctcgaagt agctcgccgc gaacgtgttg cgtgcgatgt
    12001 tgttcgatac gaggtcgttc gcgtacgcgc cgcgcagcaa caggtcgatg ttttctgcga
    12061 tcagcgtgcc ggcgcggccc gagacgagca gctgcggcac gaccgggccg aggtcgaggt
    12121 agcgtttcgt ctcgagcgga tcgttctgcg gcaccgacgg atcccagcgc cagtcggtgt
    12181 cgcgacgatg cgtgacgtcg aacacgaggt tcgtcacctg cttgtagcgg ctgcgcagct
    12241 gcacgtgctc gttcgcgagc acaccgtcga gcgtgcgcgc cgagccgatc acggcaatgt
    12301 ccttgcgcgg ccgccagtcg atctggatct ggctgtgctg cgacgctttg tcggcggcgg
    12361 caccgaccgc ggtgaacgcg aggaactcac cgctgatcgc aaacggaatt ggcgtcttca
    12421 aggcgcgaaa gtcgatgcgc agtgacgagc caccgatacc cgcgcggttc ttgccgtcag
    12481 ggcggtcgac gcgcgtgtag tccgcgacgc gaaaaccgct gtacaggctg ccgcggatca
    12541 atttgccttc ccaggcggcg accgcaccga ccatgtgcag catccacggt ccatagacgt
    12601 agaggctgcc ggcgcgcagg cgcaacggag cgagtcgctt gtcgggcaag aagtcctgca
    12661 cctcgcccca gccctcgcgc aaatcgaagc cgctgcgatc gaaccagttc gcgatcggcg
    12721 gcgcgacacg cacgtccttc ggcgtcggtg ccatcggatc gaacgacatc gtgctgcgcg
    12781 tcagctggaa ccggctcgcg aaataggtcg agaggctctg caacatgaca ccgcgcgagc
    12841 tgaagtagcc ctcgccgagc gcgtacgcat gtagctgcga gaactcgtag ggctcgagcg
    12901 gcttgccggc aaggttgtcc ttgccggtca acgcggtgcc gtcgacgacg tagcccatgt
    12961 tgaaccgcac cgagaccggg cggcgcagat cgcgaaccga gccgagctcg accgcgggct
    13021 cgcgtgcggc cgcttttacc gaggcgtcct cggcgggcgc cggtgtgagc gcggacgcag
    13081 gtgcagggtc tggcttgctc gccgcggcag tccgcactcg catcttgaga ttgcgcggcg
    13141 gcggctcgtg cgtgagcgtc agcttcgccg gctcggcgcg cgcggtcgca gtcggcacga
    13201 ggacgagcgc gagcacgaac gcgaatgctc gcgtcatggt ctcaccgtgt ggcagcccga
    13261 gcccatgcac gtcgtctgct ggtcgtgaca ggtcaagcac gagcgcacgt tgacgcgcgc
    13321 gatgccaccg tgctcatcgc cattcgcgaa catgcccgga aagccgtgcg agcgcggacg
    13381 ctgcgcgtga cacgcggtgc agaactcgat gacgtgacag cgcgcgcagc gatcgcgatc
    13441 ggctgccgcc tccgcgccgt gatcgagctc gcggaacgtg atgcggtgat cgctcggccg
    13501 catcgtctga tggcactcgt cgcagttgtc gcggccgttg ccggacatct gggtgtggca
    13561 cgcaccgcag cgagcaccgt cacgctccgc ggcctccgcg tgatcgcggc ggaatgccaa
    13621 cgtgtgatcg agaggacgct cggttgccgg caggtgattg cgcggagcga gcgacgtcag
    13681 tgtctgcgtg cgctgactgt ggcaggtctc gcacgcgcgc atgcgcagct cggccggcgt
    13741 gcgctgggtg tcgtcgtgac agccgacgca acgctcgatg cgcggcggtg cgtgatcggt
    13801 gtacgtcgtc gacgccgcac tttgcgcgtg gcactcctcg cagcgaattg cggtgccgcg
    13861 gcggtcggac ttgtggtgct cgtgatcgaa gcgcaggtcg ttcttgatca gtcgcgcgcg
    13921 cgtgcgcagg ttgacgccgt tcttgtgaca gccgttgcag ctcgtcatcg cgggcgcgtt
    13981 gtcgagatgg gtctcggggg cgtggcaacg cgcgcacgtc gcgtggtttg gcgtcgcgag
    14041 gctgccgtcg cggacgtgac agtcggcgca cgccacgtgg aagccgacgc gaccttccat
    14101 gcgcgagaag tccatgtgga tcgcatgcga gaaccgcacc ggctcggcgc gccagatgtc
    14161 ctcgatcgga tacgggcgca gcggtgccgc tagcggcgtc gcggtgacct gcgtgtggca
    14221 gaccttgcag agctcgcccg gggctgagag aaacgcctgc ttgtggcagc cgtcgcacgg
    14281 cttgtgatcg tcactgccgg gacgcttgtc catcgcgtgg cacgtctcgc acttgatctg
    14341 cgcgtgctgg gaatgcgaga ggcgcgtctt gtcgatcggt ggcgccggcg cttcgatcgt
    14401 gcgcgccgac gagcagccga tcagtaccag gactgcaatg aggaacgagc gagccatccc
    14461 cgaggccgcg tcgacggtaa cacgagcgca aacctcgtac gaccgcgcaa ggcccgtaca
    14521 gtgcggaacc cagatcctgc gtagtttgcg tgccgatcac cgggaacacg cgttgcaatc
    14581 tgcctagaca tgcgaacgag cgaggtggac ccgaacgcct ccggcgagat cgaaatcccg
    14641 ccgccggcca agccgcagcg tgttgtcgac aagaagacgg tggttgtcga caaacgcggt
    14701 ctgttaccga tccgcaagcc acgcgcgctg acgatcaagg agtagtgagc ggcggcagcg
    14761 ttcgagtgag cgctgcgatg cggtcctcgt gcgtgagatg aggttgccag tcggtcagca
    14821 agcgacgccc gcggacaagc cgatcctcgc cgtccggtcg ccaccacgcg accgcgaagc
    14881 cgcgcacgcc ctctggcgtg tacgcgatca ggacgcgatg accatgcagc tccgcgagca
    14941 ccggctcgag cgcgtcgagt gccgcgtgga cgacgaccat gccgtcgggt gcgacgaacc
    15001 ctcgcgcgtg cacgaacatg cgctcgatga acgtcgcgtc ggtcatccgc cagcgcgcgc
    15061 gataggggtc gccgccaccg ggttccggaa tcggtgcgtt gcaggtgacg aggtcgcagc
    15121 tgccgcgtag ctcgggcgcg atgccgt
//
LOCUS       DASUFX010000074         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1401198, whole genome shotgun sequence.
ACCESSION   DASUFX010000074 DASUFX010000000
VERSION     DASUFX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401198"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFV99_02830"
     CDS             <1..324
                     /locus_tag="VFV99_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5058263.1"
                     /translation="VVRAPGYVAAAQQVTVRSGRGMQDATFDLVRGATLAGEVRDRFG
                     RRVAGAKVSIGDVSTVTDGNGEFRLSGVESGTLAAESEGTRAALDLRLAPGDERLSLT
                     LNLSE"
     gene            complement(355..828)
                     /locus_tag="VFV99_02835"
     CDS             complement(355..828)
                     /locus_tag="VFV99_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058264.1"
                     /translation="MRRILLPLLLTGTWAACASDDPAPIVHVLSATPDQIDPADDLHD
                     DVRITVEYEDADGDLGEGVAEIHDCRDDTLTTVLPIPAIAPDDVVSDGAKITGSLDLY
                     LTDVGAATAATLPSVCADLGITALGADETVFCVVLVDQKEHAGPGDCTEAITLAP"
     gene            complement(837..1769)
                     /locus_tag="VFV99_02840"
     CDS             complement(837..1769)
                     /locus_tag="VFV99_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058265.1"
                     /translation="MKRLVYIALALGTLGACGNDERHDGDVDAGVDPDPLEVTATAAP
                     GSLDDLHQRIIARSCSGQPGLCHNGQFEPNLSTPALMYAYLVNRPGIEKDDRLRVKPG
                     SAAESLFIDKIRHRNGVATQMPLGAPPLEEEDIAELEAWINAGALREPGAAPAPNLNN
                     PPKRPQIGIFNGTTRLDGAGPVTVSVNTTLTLRHTVHDFETADNAIPFAAVILQIGDG
                     RQIVLDAAAQDPHVGPTTYDAAGPPAQGDVFNYKRTWTIPTTLSLRDPDTAAMSTAPA
                     SGQTVTIICVYIDGAPQQDGIVAIEIGATPIQIQ"
     gene            complement(1766..3202)
                     /locus_tag="VFV99_02845"
     CDS             complement(1766..3202)
                     /locus_tag="VFV99_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5058266.1"
                     /translation="MALSRRNLLKGLGAVAAATTLSPLIRVKRASAAGNGKRVVIVGI
                     GGGLRLRESLGMGEGATFPNLFGTTPLVTGFATGSQGAPRIAPEYAATAQPLALPAPR
                     ATPLHTLGTLVTNLRYADGPPGHLQGHGCLLSGFYNQLENRADAHLPVPTIFELHRRA
                     TNAPATDAWYVSMVGGFYRALITSDNPGYGPKYAGVYLQPPGAMQPLVPILTSGKRTI
                     DVQPGALPTIPRNAAEDAAVDRLTKILDGNSPPFSPTDPIVHLADEDNARIQNHLAEI
                     YSDGTYQSFFADSFGIGLRKTDGTIDGTNDAKTIYHAERVLSKFKPAVMAITLLDIDV
                     CHMDFNGYLRSQQLADAMVTHLWNFIQADTELKDTTTLIIMPEHGRHLFFNGNNPDSL
                     GRSGIDHGQGDNGDRDVWMLALGPDIKPNQVIAPTGIVQSGRTSGRYETIDSIMTAMS
                     VLGHETAMKDALVGENARPGLLMQEVMA"
     gene            complement(3212..3724)
                     /locus_tag="VFV99_02850"
     CDS             complement(3212..3724)
                     /locus_tag="VFV99_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058267.1"
                     /translation="MMRLLLIVALAAACTDVYDYDAASAGEPEGSERAPRGKTSTQFL
                     RGIYADLLGRTPESYEIVIKFQGTEVFRFTADEETLLSVSLDGLGDSQPMRNLIANGL
                     LYSAEVQIPDKGAVTARDFIRDQFRRLLGREPNTYELEAFAAEWDKDPAVGPRAIIRA
                     IVASREYQSQ"
     gene            complement(3721..>4226)
                     /locus_tag="VFV99_02855"
     CDS             complement(3721..>4226)
                     /locus_tag="VFV99_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058268.1"
                     /translation="DSCKCACNILPQLDTERTDLMKATADFKANLATSDIERRYAMAT
                     GYFILNGAPEARVTALFDDFLSRPAEADEIENGRAMIFGAIFPGAPAGLMFHRHGSNY
                     ADLIDIIFDSDVYRESLTRRVFNRYLAREPSAVELAYFPTTLDATKPDMRSLVRAVLS
                     SREYFDQ"
BASE COUNT          646 a         1320 c         1493 g          767 t
ORIGIN      
        1 gtggtgcgcg cgcccggtta cgtcgccgcc gcacaacagg tcaccgtgcg cagcggtcgc
       61 ggcatgcaag acgcgacgtt cgatctcgtg cgcggcgcga cgctcgccgg cgaggtccgc
      121 gaccgattcg gccgccgcgt cgctggtgcg aaagtatcga tcggcgacgt ctcgaccgtc
      181 accgacggca acggcgagtt ccggctctcc ggcgtcgagt ccggcacgct cgcggccgag
      241 agcgagggta cgcgggccgc gctcgatctg cggctcgccc ctggtgacga acggctgtcg
      301 ctcacgctaa atctgtccga gtgaagcgca cgatagacga gaacggcaac tacgtcatgg
      361 tgccaaggtg atcgcctcgg tgcagtcgcc cgggcccgcg tgctccttct gatcgacgag
      421 cacgacgcag aacacggtct cgtccgcgcc cagcgccgtg atgccgaggt ccgcgcacac
      481 ggacggcagt gtagccgcgg tcgcggcgcc gacatcggtg agatagagat cgagcgagcc
      541 ggtgatcttc gcgccatcgg acacgacgtc gtccggcgcg atcgccggaa tcggcagcac
      601 agtcgtcagc gtgtcgtcgc gacagtcgtg gatctcggca acgccttcac cgaggtcgcc
      661 gtcggcatcc tcgtactcga cggtaatgcg gacgtcgtcg tggaggtcat ccgcaggatc
      721 gatctgatcg ggcgttgccg atagcacgtg cactatcggc gccggatcat cgctggcgca
      781 ggcggcccag gtacccgtga gcagtagtgg gagcaggatg cgtcgcatgg tggtcctcat
      841 tggatctgga tcggcgttgc accgatctcg atggcaacga tgccgtcctg ttgcggcgca
      901 ccgtcgatgt acacgcagat gatcgtgacg gtctggcccg atgccggcgc cgtcgacatc
      961 gcggcggtgt ccggatcgcg gaggctcagc gtcgtcggga tcgtccatgt gcgcttgtag
     1021 ttgaagacgt cgccctgcgc cggcgggccg gctgcgtcgt acgtcgtcgg gccgacgtgc
     1081 gggtcttgcg ccgccgcgtc gagcacgatc tggcggccgt cgccgatctg caggatcacc
     1141 gcggcgaacg ggatcgcgtt atccgcggtc tcgaagtcgt gcacggtgtg ccgtagcgtc
     1201 agtgtcgtgt tcacgctgac cgtcacgggg ccggcgccgt cgaggcgcgt ggtgccattg
     1261 aagatgccga tctgcggccg cttcggcggg ttgttgaggt ttggtgccgg agcggcgccg
     1321 ggttcgcgca atgcgccagc gttgatccac gcctcgagct cggcgatgtc ttcttcttcg
     1381 agcggcggcg caccgagcgg catctgcgtc gcgacgccgt tgcgatggcg gatcttgtcg
     1441 atgaacaagc tctccgccgc gcttcccggt ttcacgcgca ggcgatcgtc cttctcgatg
     1501 cccggccgat tgacgaggta tgcgtacatc agcgcgggcg tcgagaggtt gggctcgaac
     1561 tgaccgttgt ggcaaaggcc gggctgcccc gaacagcttc tcgcgatgat ccgctgatgc
     1621 agatcgtcga ggctgcccgg tgcagcggtc gcggtcacct ccagcggatc gggatcgacg
     1681 ccggcatcga cgtcgccgtc gtgccgctcg tcgttgccac acgcgccgag cgtgcccagc
     1741 gcgagcgcga tgtagacgag gcgcttcatg ccatcacctc ctgcatgagg aggccgggcc
     1801 gtgcgttctc gccgacgagc gcgtccttca tcgcggtctc gtgaccgagc acgctcatcg
     1861 ccgtcatgat cgagtcgatc gtctcgtaac ggcccgaggt gcggccgctc tgcacgatgc
     1921 cggtcggagc gatgacctgg tttggcttga tgtccgggcc gagcgcgagc atccacacat
     1981 cgcgatctcc gttatcgccc tggccgtggt cgataccgga gcggccgagg ctgtcggggt
     2041 tgttgccgtt gaagaacagg tgacggccgt gctccggcat gatgatcagc gtcgtcgtgt
     2101 ctttgagctc ggtatcggcc tggatgaagt tccacaggtg cgtgaccatc gcgtcggcga
     2161 gctgctggct gcgcagatag ccgttgaagt ccatgtggca gacgtcgata tcgagcagcg
     2221 tgatcgccat gacggccggc ttgaacttcg agagcacgcg ctctgcgtga tagatcgtct
     2281 tcgcgtcgtt cgtgccatcg atggtgccgt cggtcttgcg tagaccgatg ccaaagctgt
     2341 cggcgaagaa cgattggtac gtgccatccg agtagatctc ggcgaggtgg ttctggatcc
     2401 gagcgttgtc ctcatcggcg aggtggacga tcggatcggt cggcgagaac ggcggcgagt
     2461 tgccgtcgag gatcttcgtc aggcggtcca ctgccgcgtc ctccgccgca ttgcgcggaa
     2521 tcgtcggcag cgcaccgggc tgcacgtcga tcgtgcgctt gccgctcgtg aggatcggga
     2581 cgagcggctg catcgcgccc ggcggctgca gatagacgcc ggcgtacttc gggccgtagc
     2641 ccggattgtc gctcgtgatc agcgcgcggt agaagccgcc gaccatcgac acgtaccagg
     2701 cgtcggttgc cggcgcgttc gtcgcacggc ggtgcaattc gaagatcgtc ggtacgggca
     2761 ggtgcgcgtc ggcgcggttc tcgagctggt tgtagaagcc cgagagcaag cagccgtggc
     2821 cttgcaggtg gcccggcggt ccgtcggcgt agcgcaggtt cgtgacgagc gtgccgagcg
     2881 tatgcagcgg tgtcgcgcgc ggcgccggca acgcgagtgg ctgcgcggtc gccgcgtact
     2941 cgggtgcgat gcgcggcgcg ccctgcgatc cggtcgcgaa gccggtgacg agcggcgtgg
     3001 tgccgaacag gttcggaaac gtcgcgcctt cgcccatgcc gagcgattca cgcaggcgca
     3061 ggccaccgcc gataccgacg atgacgacgc gcttgccgtt gcccgctgcg ctcgcgcgct
     3121 tgacgcggat gagtggtgac agcgtcgtcg cggccgctac cgcgccgagc cccttcaata
     3181 gattgcgtct gctgagtgcc atggctcgct cctattggct ctggtactca cgcgacgcga
     3241 cgatcgcgcg gatgatggct cggggaccga cggcgggatc cttgtcccac tcggcggcga
     3301 atgcttcgag ctcgtacgtg ttcggttcac gcccgagcaa gcggcggaat tgatcgcgga
     3361 taaagtcgcg cgcggtgacc gcgcccttgt cggggatctg gacttccgcc gaatagagca
     3421 ggccgttcgc gatcaggttg cgcatcggct gtgaatcgcc aaggccgtcg agcgagacgc
     3481 tgagcagcgt ttcctcgtcg gcggtgaagc gaaacacctc cgtgccctgg aacttgatca
     3541 cgatctcgta gctctccggc gtgcggccga gcagatcggc gtagatgccg cggaggaact
     3601 gcgtggaggt cttaccgcgc ggtgcgcgct cgctcccctc gggctcgccg gcactcgccg
     3661 cgtcgtagtc gtagacgtcg gtgcaggcgg ctgccagcgc gacgatcaac aggaggcgca
     3721 tcattggtcg aagtactccc gcgacgacag cacggcacgc acgaggctgc gcatgtccgg
     3781 cttggtcgca tcgagggtgg tcgggaagta cgcgagctcg acggcgctcg gctcgcgggc
     3841 gagatagcga ttgaacacgc ggcgcgtcag cgactcgcga tagacgtcgc tgtcaaagat
     3901 gatgtcgatc agatccgcat agttcgaacc gtggcgatgg aacatcaggc ccgccggagc
     3961 gccggggaag atcgcgccga agatcatcgc gcggccgttc tcgatctcgt cggcttccgc
     4021 gggacgcgac aagaagtcgt cgaacagcgc ggtcacgcga gcttcggggg cgccgttgag
     4081 aatgaagtag ccggtcgcca tcgcgtagcg acgctcgatg tccgacgtcg ccaggtttgc
     4141 cttgaagtcg gcggtcgcct tcatcaagtc ggtacgctcg gtgtcgagct gcggcaggat
     4201 gttgcatgcg cacttgcacg aatcca
//
LOCUS       DASUFX010000075         4009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_428850,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000075 DASUFX010000000
VERSION     DASUFX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428850"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..758
                     /locus_tag="VFV99_02860"
     CDS             276..758
                     /locus_tag="VFV99_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058269.1"
                     /translation="MAPATLNVFVPTPSRSIVAGATLVSQLLGTLAFALALVWIIKHG
                     YRGPINVTILVLGAVAVFAGGNAHRGSVVALVVCAVIDLAVAVACLANISAVDTFVHT
                     SVAWAAPAVVPKLHIMTVIAGIVAVLAASACMAAVPQTRRFAAWRERQILHAARAWNS
                     "
     gene            789..2213
                     /locus_tag="VFV99_02865"
     CDS             789..2213
                     /locus_tag="VFV99_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX5058270.1"
                     /translation="MSPFVRAPVVRIVDHTDTITVLGPKGARTFSGDSAELVRAVLEI
                     VAAPTTRAELRAELEQRAGEAVPETLVDELIGVLVADGALVAPEPARPKPVAVRRVVL
                     GITGAVAAVDAPALVRGLQSIGCEVRIALSKSAKKFVSVAALEALTHHAVWHGIWRRD
                     REMPVPHINLAEWAELFIVCPASATTLARIATGDCSDLVAAIAAATRAPVVLVPSMND
                     AMYESPAVQQNLTTLRAHGRYLVHPAMGHEVAHAPDERRSLLGPAPPAHALVDIVRHL
                     LAQSGPRVLPVGANAWERLWATATLEQLPWHAESVDGPLATALAERCAVGRKLIDLGT
                     GAGTVAIEAARRGYAVTATDIAASALRRARERAGALPIQLVLDDITATCIDTRYDVAV
                     DSGLFHCLPRERWDAYAAAIDRLVAPGGALLLVAHQPGTQLATNPVTDAELRVLLPGF
                     EIARTAPTTLSGGNATLFELEHRL"
     gene            2221..2481
                     /locus_tag="VFV99_02870"
     CDS             2221..2481
                     /locus_tag="VFV99_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058271.1"
                     /translation="MKPRRALRVPVALVFTVGTAAAVVASAASVSCGGDDDTKKDGGS
                     CGVFCFASQNDGGVPPPDGGCPLCADFSSGTPVCPAGCDPLG"
     gene            complement(3196..3306)
                     /locus_tag="VFV99_02875"
                     /pseudo
     CDS             complement(3196..3306)
                     /locus_tag="VFV99_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(3566..>4009)
                     /locus_tag="VFV99_02880"
     CDS             complement(3566..>4009)
                     /locus_tag="VFV99_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058272.1"
                     /translation="LPHHTLGAALRLEGKCREAIPHFLEAKAIWEKTLGTSHHQTSAA
                     YFDLGVCYTDLGRVQEALPLLEKTVEIRVAANRSNGAIAEAKWQLGRALWESGKDKPR
                     GLALVQESQRKLAGLRQGREIDTWLAAHKLAPAAPPPVAPTPQPK"
BASE COUNT          594 a         1279 c         1401 g          735 t
ORIGIN      
        1 tcacggtgcg cgcgcggtta ggtctgtaac gcgtgatgca ttcgtcagcg aacgtgaagg
       61 tggcacgtgc gagtgccaca ccggacaagg tgtgtgcagc gctcggattc ttttctgcga
      121 ccaaattggt gattttttca cagcgtgagt gagagcgcgg ctttcgctat cgtgcgctcg
      181 tgggtgagat gcggtctctt cattgtgagt gcggcgcgac gcgcgaagca ccggtgggca
      241 tcaggatcct cagctgcgtc aaatgcggcc gagcgatggc gccggcaacg ttgaacgtgt
      301 tcgtgccgac accgtcgcgc tcgatcgttg cgggcgcgac gttggtgagc cagctgctcg
      361 gcacgctcgc gttcgcgctg gcactcgtgt ggatcatcaa acacggctat cgcgggccaa
      421 tcaacgtgac gatactggtg ctcggtgcgg tcgccgtgtt cgcgggcggc aacgcgcatc
      481 gcggcagtgt ggttgcgctc gtagtgtgcg cggtgatcga tcttgcggtt gcagtcgcgt
      541 gcctcgccaa catctcggcc gtcgatacgt tcgtacatac atcggtcgcc tgggccgcgc
      601 cggcggtcgt tccgaagcta cacatcatga cggtgattgc cggcatcgtt gcagtgcttg
      661 cagcaagcgc gtgcatggcc gcggtcccgc agactcgtcg gtttgcggcg tggcgcgagc
      721 ggcaaatcct tcacgcagca cgcgcctgga acagctagcg gacacggttt cggcgttatg
      781 ctgcctcggt gtcaccgttc gttcgcgcgc cggttgttcg catcgtcgac cacaccgata
      841 cgatcacggt gctcggcccc aaaggagcgc gcacgttcag cggcgactcg gccgagctgg
      901 tgcgtgcggt gctcgagatc gtcgctgcgc cgaccacacg cgccgagctg cgtgcagagc
      961 tcgagcagcg cgccggtgag gcggtacccg agacactcgt cgatgagctg atcggcgtgc
     1021 tcgtcgcgga cggagcgctc gtcgcgccgg agccggcgcg gcccaagccg gttgccgtgc
     1081 ggcgcgtcgt gctcgggatc accggtgcgg tcgctgcggt cgacgcgcct gcgctcgtgc
     1141 gcggcctgca gtcgatcggc tgcgaggtgc gcatcgcgct ctcgaagagc gcgaagaagt
     1201 tcgtgtcggt tgcagcgctc gaagcgctca cccatcacgc ggtgtggcac ggcatctggc
     1261 gtcgcgatcg cgagatgccg gtgccgcaca tcaacctcgc cgagtgggcc gagctgttca
     1321 tcgtgtgtcc cgcgagtgcg acgacgctcg cgcgcattgc gacgggtgac tgctcggatc
     1381 tcgtcgcggc gatcgcggcc gcgacgcgtg cgccggtcgt gctcgtgccg tcgatgaacg
     1441 acgcgatgta cgaatcgccg gccgtgcagc aaaatctcac gacgctacgc gcgcacggcc
     1501 gttacctcgt gcacccggcg atgggccacg aagtcgcgca cgcgcccgac gagcggcgct
     1561 cgttgctcgg tcctgcaccg ccggcgcacg cgctggtgga catcgtgcgc catctgctcg
     1621 cgcaatcggg gccgcgtgtg ctgcccgtcg gtgcgaacgc gtgggagcgg ctgtgggcaa
     1681 cggcgacgct cgagcagcta ccgtggcacg ccgagagcgt cgacgggccg ctcgccaccg
     1741 cgctcgccga gcgttgcgcc gtcggccgca agctgatcga tctcggtacc ggcgcaggca
     1801 ccgtggcgat cgaggctgcg cgccgcggtt acgcggtgac cgcgaccgac atcgcggcaa
     1861 gcgccttgcg acgggcacgc gagcgcgcgg gtgcgctgcc gatccagctc gtcctcgacg
     1921 acatcacggc gacgtgcatc gacacgcggt acgacgtggc agtcgacagc ggcctgtttc
     1981 actgtcttcc ccgcgagcgc tgggatgcct atgccgcggc gatcgatcgc ctcgtcgcac
     2041 cgggaggcgc gctcttgctc gtcgcgcacc agccaggcac gcagctcgcg acgaacccgg
     2101 tcaccgacgc cgagctccgt gtgctcctgc ctggtttcga gatcgctcgc acggcgccga
     2161 ccacactgtc gggcggaaat gcgacgctct tcgagctcga acaccggcta tgatcgcgcg
     2221 atgaagccgc gccgcgcgct ccgtgtcccg gtggccttgg tgttcacggt cggcactgcc
     2281 gcggccgtcg tcgcaagcgc cgcaagcgtc agctgcggtg gcgacgacga caccaagaaa
     2341 gacggcggca gctgcggcgt gttctgtttc gcgagccaga acgatggtgg ggtgccgccg
     2401 cccgacggag gttgcccgct ctgcgccgac ttctcgtcgg gcacgcccgt gtgtcccgct
     2461 ggttgcgacc ccttgggttg atgaagtcat cgccgctcgg tcgagcggca agcatcgcaa
     2521 gcgctagatc ggcccgccga gcgtgtagac gacgaacgag ttgtactcgc cggtcacgcg
     2581 gttcgtgcga aagccaggga tgccgctggc gttctggttg ctcgtgaacg gtgcggtcgg
     2641 tccggtctgc agcgagtcga gcgcgcagta gtgattggat ccgtacgcgc cggcaaggat
     2701 ccgataacgg ccgcccgggg cgacatcggg gatcgtggcg cgattgcgtt cgaagtttgc
     2761 ggctccgttc tggatggtcc agatcatgag caatgacatc gcgggcgtga tcgtgtcgtc
     2821 gtgaaacacg gcgcatagcc agccgttgtt gcccgtaatc cgccacagga tgccgccatt
     2881 gacgttgccg gtatcgaggc tcgcgaacgt gaacgtcgcc tcggcgatgt agtcgttgac
     2941 cgcgatcgca gccggattgt gcatgcgctg gtcgctgacc aagctcatct gctgcacggc
     3001 gtcacccgag acgggccacg tgccgttgat gatcgtccag tcggggctca gcgtcgtgcc
     3061 gacgaacggc tcgaagtagg cgatcgactc gccgctctgg gtcggacgcg gatcgcacgc
     3121 gtcgccgacc gaatccgcgg tctgcccggc gtttgtttcg ccgaggttcg cctggttcgt
     3181 gttcgccacc gtcgggcagt tgtcgcaaac gtcgccgtag ccgtcggtgt cttcgtcgtg
     3241 ctgggccgga ttctggatcg agacacagtt gtcggtgtcg tcgaacacgc cgtcgccatc
     3301 ggtgtcgagc gtcgggtcac cgcgcggcgc atcgaagtca gggttcggat cggcatcgat
     3361 gggacagttg ccgggcacgc aggtacagtt gcccgacacg cagtcggcgg gcggcacgag
     3421 gtcgaagttg aaccgacatg ccgtgagcgt cacgctaagc gcgaaggcga ctgctcgcat
     3481 tgccacgatc atagagtcga tcgaacttcg acgcgcagca atcgttgtga tcttcctgac
     3541 gttcgctgcc acaaatcgcg gcggctcact tcggctgcgg ggtaggcgcg acggggggtg
     3601 gagccgcagg tgccagctta tgcgcagcca accaggtgtc gatctcgcgg ccttgacgaa
     3661 gcccagcgag cttgcgctgt gattcctgta cgagcgcgag tccgcgcggc ttgtccttgc
     3721 ccgattccca cagcgcgcgg ccgagctgcc actttgcctc ggcgatcgcg ccgttcgagc
     3781 gattggcggc tacgcggatc tcgacggtct tctcgagcag cggcagcgct tcttgcacgc
     3841 ggccgaggtc ggtgtagcag acgccgagat cgaaatacgc ggcggaggtc tgatggtgcg
     3901 acgttccgag cgttttctcc cagatcgcct tcgcctccaa gaagtgcgga atcgcctcgc
     3961 ggcattttcc ttcgaggcgc agcgcggcgc cgagcgtgtg gtgtggcag
//
LOCUS       DASUFX010000076         9483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1958097, whole genome shotgun sequence.
ACCESSION   DASUFX010000076 DASUFX010000000
VERSION     DASUFX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958097"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VFV99_02885"
     CDS             <1..1236
                     /locus_tag="VFV99_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5058273.1"
                     /translation="DAGTMCPSYMATLDERHSTRGRAHLLFEMMNADVITDGWRSEEV
                     REALDLCLACKACKRECPTHVDMATYKAEFMSHYFAGRIRSREAYVFGWLHRWLVLGR
                     AMPRLANFATHAPLLRAIAKWIAGIAPEREIPRIARHSFRHSFFPPVAANGRTRVLLW
                     PDTFNDTFFPSVLHDAARVLTHAGFAVDIPRRRLCCGRPLYEYGWVKQARHLWQRTLH
                     DLADDIDDGVAVVGLEPSCVASFRDELPALFPDDPRAARLAKQTYSLAEFLATTPYTP
                     LRVRPQHVLYHRHCHQAAVLEPEHDIRLLRAAGHRLEVLDSGCCGLAGAFGFQRAKYP
                     ISVALAERVLLPAVRANPDAVVVADGFSCREQLLQLGGVRALHLAELLADVPALVPAV
                     VPSDAELDRIRAAAARTLR"
     gene            complement(1233..1796)
                     /locus_tag="VFV99_02890"
     CDS             complement(1233..1796)
                     /locus_tag="VFV99_02890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5058274.1"
                     /translation="MPALTTERLRLVPITLAMIEGVMRRDRAAAEAALDACIRDCGLD
                     LPACARFPDAWPNEELVARAFPFSLEAIRADPDTRLWGDRLVLPLDGPPRVVGSVVFK
                     GRPADGIAEVGYGIEDGSRSQGLATEATRACVNWALDQAGILAVHATTFPWHHASLGV
                     IRKLGMVQCGTREHDMLGELLVFELKR"
     gene            complement(1796..2350)
                     /locus_tag="VFV99_02895"
     CDS             complement(1796..2350)
                     /locus_tag="VFV99_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058275.1"
                     /translation="MRRVLAAAAVIGALASPVAADPLPPGTIGFLFGVLSGTGADAKR
                     LGYGMYQFGMQAAWQPAATERSWGWSLRWSTLFGTLYNGTAPQIDTELHTVQMDLQVG
                     LRVRPWDTPSRYLTAHVGGELLRTNEPVPPLMQRVFAGGVATVGLDQYVGGFMFNVDV
                     RYGLIGNDGPQELALLVGVGLTGP"
     gene            complement(2353..2856)
                     /locus_tag="VFV99_02900"
     CDS             complement(2353..2856)
                     /locus_tag="VFV99_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5058276.1"
                     /translation="MGEEPRPSELDIKGKSTLEILSQIDLFSGLPLGHLRRVVDIGVE
                     EQYRNGATIFSEGEPGDKFYLIVEGAVRISRIVPGMGEEALAVLRPGNYFGEMSLIDD
                     APRSATAMCHEKCRLFVVNRRDLEDLLFVDRDLAYELLWNWVRTLSRRLRATNDKMTF
                     LATTSKF"
     gene            complement(2951..3313)
                     /gene="atpE"
                     /locus_tag="VFV99_02905"
     CDS             complement(2951..3313)
                     /gene="atpE"
                     /locus_tag="VFV99_02905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX5058277.1"
                     /translation="MKTKNIISFLTVLLILGISAVAFAQPEAAGAAFDAAAVKADGLA
                     KNFGLIALAAGLGIGIAALGGGIGQGRAAAAALDGIARNPGAAGQIRGPMILGLALIE
                     SLVIYALIISLLLVLKVG"
     gene            complement(3400..4179)
                     /gene="atpB"
                     /locus_tag="VFV99_02910"
     CDS             complement(3400..4179)
                     /gene="atpB"
                     /locus_tag="VFV99_02910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5058278.1"
                     /translation="MGEHGTWFDYLNRFSWWQDFNHWAQGTLGRKWKWMMFDESGWTL
                     THVLTTALVIMFCVWGAMSFYRGTKSKDKGLVPPRKMNLRNFFEYTAESVYGMVEGAM
                     GEANAKKFFPLIGALWMFILFGNLIGLVPGFVTPNDTLKTNVGIAVMVFLLTHYYGIK
                     EHGPINYFKHFMGPSPALAPLIFPIEMISHFARPLSLSVRLLGNMMADHKVVMSFFAL
                     VPILVPLPFMLLGLLVCLIQALVFCTLTMVYISMAIEHEEH"
     gene            complement(4172..4576)
                     /locus_tag="VFV99_02915"
     CDS             complement(4172..4576)
                     /locus_tag="VFV99_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5058279.1"
                     /translation="MATPSLLRIERMNYALGGVLVIAAAVTQPQDVALGIAVGVALTC
                     LNFFVLRKLVFKWTSDAAKGKTGNAPLLMLPKMIGLMGAVAVAILFLPIDPIAFTIGY
                     SLFIVSIVLDTTYSALRPQAEPPSTEHDEHHG"
     gene            complement(4563..4859)
                     /locus_tag="VFV99_02920"
     CDS             complement(4563..4859)
                     /locus_tag="VFV99_02920"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5058280.1"
                     /translation="MDRRSSRAADPAARNAKRAYNALNASSVGLELGLSVGIGLLVGY
                     YMDKWLGTQPWLMLLWLLLGLIAGFRGVFRAVARADRAAEAQANEDKGGGHGNA"
     gene            complement(5001..6278)
                     /gene="hemL"
                     /locus_tag="VFV99_02925"
     CDS             complement(5001..6278)
                     /gene="hemL"
                     /locus_tag="VFV99_02925"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5058281.1"
                     /translation="MGIQRSQSIFERAKQVIPGGVNSPVRACRSVGVDPVFVAQGDGA
                     YITDVDGNRYIDLIGSWGPLILGHSQPEILDAIAEAMSQGTTFGAPTEIEVRFAERIR
                     ESYPSMEMVRAVSSGTEATMTALRLARGFTGRNKILKTDGGYHGHADCLLVSAGSGVA
                     TLGIPGSAGVPDGAARDTLVVPYNDLAAVEATFAANPGEIAAIIIEPIAGNMGLVLPQ
                     PGYLQGLRDLTTKHGALLIFDEVITGYRVGYRGAQGLFGITPDITCLGKIVGGGLPAA
                     AFGGRADIMQKLAPLGPVYQAGTLSGNPLAMAAGLKALHILGRPGNYERLDQLGKRLG
                     DGLLAAAQAAGVPACVNRIGSMVTLFFANGPVTDYATAKTSDTARFGAFFRKMRDKGV
                     FLPPSQFEAMFVSLAHTEEDVDSVIAAARDSLS"
     gene            complement(6320..7222)
                     /locus_tag="VFV99_02930"
     CDS             complement(6320..7222)
                     /locus_tag="VFV99_02930"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5058282.1"
                     /translation="MLFHRFIALVATALALTMTSAHVLEMAPKLAYSYELYTAVNGTL
                     YRYFAIVGGAYTIVALIAVVTLAWRVRRQRSAHWTLLAALAVLASFVSWLVLVVPVNQ
                     QIAEAGPSAVEAWVTLRPRWEYGHLIGFVLTLVGFCALVIATLVDIPRAHGIHAEVSR
                     IVHAPPERLMALYMDYTAWPRLFPATIRGVRWVRTDGPAITVEVDHASAGTVPNVITP
                     TSEREIQLDEKKPSYQARFLNRFEPVPGGTRYTVIADIAPRGRLRALAWLARPFVRSR
                     IRRFVVEPMRARAEQEQLAATAPA"
     gene            complement(7251..8153)
                     /locus_tag="VFV99_02935"
     CDS             complement(7251..8153)
                     /locus_tag="VFV99_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5058283.1"
                     /translation="MKPIAALLIVMLLASCYREKRDNQTLQRLAQIEQRLDAQDKALA
                     ELRAQPGTTELTALAGQLADLHDQLDTLLDAVKQAKPARGQRREPDPAVTYSVPLGKS
                     PVYGSPKAKVTLVMAMDFDCPFCRKAWDTVEQLRQKYGPDLRVAYKPYVVHPKTAKLA
                     AEAACGAQKQGKWREMAQLIWTQAFDQRANNSDAFSPAHMTALAKQLKLDMNRYAQDV
                     AGVCVQEVRDEMATMTRLGVGATPTFFINGRYVAGALPIDKFSPLIDEELAKATAAEK
                     RGVKPEQYYEQEIVGKGTAQLAQP"
     gene            8177..9316
                     /locus_tag="VFV99_02940"
     CDS             8177..9316
                     /locus_tag="VFV99_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058284.1"
                     /translation="MRWGLVGLVLFVCACDSRYGAYFVIKSDVEFDAVEVYFGTPIDS
                     SGPGSGDSFATPTFGAQRGTLFDRTFADADVLTVGATTETTFYVPPTEANQQLGAYVA
                     AVALSGGHPVGIAEYFGFAVPSDVVHEYQLKLAPWNPQEAERWGDTPGCLVWKHARDN
                     GTGFVAVVHGDDRDCDTLVRNADCNDLCSAGSPACAADTSFCGGATGVCALGCSANGV
                     CSPSLCLPPATCDTTCAALGNVTDRLACGAAKTSNHAEIFVERNDTLQMCAMEFLFSP
                     GVPCVMPKIEAKVPADLTGFTYAISSVPGNTMMCNLGVIQDPMTPLDDFDEHHLLISF
                     APPTNIGPRPTVVIGIQAKPNSVACANQPYYLVQMPTPPQVYACP"
BASE COUNT         1663 a         3082 c         3132 g         1606 t
ORIGIN      
        1 gacgccggca cgatgtgccc gagctacatg gcaacgctcg acgagcgtca ctcgacgcgc
       61 ggccgcgcgc acctgttgtt cgagatgatg aacgccgacg tcattaccga cggctggcgt
      121 agcgaggaag tgcgcgaggc actcgatctc tgtctcgcat gcaaggcgtg caagcgggaa
      181 tgcccgacgc acgtcgacat ggcgacgtac aaggccgagt tcatgtcgca ctacttcgcg
      241 ggccggatcc gctcgcgcga ggcgtacgtg ttcggctggt tgcatcgctg gctcgtactc
      301 ggccgcgcga tgccacggct cgcgaacttc gcaacccatg caccactgct ccgtgcgatt
      361 gccaagtgga tcgccgggat cgcgcccgag cgcgaaatcc cgcgcatcgc gcgacactcg
      421 ttccgccact ccttttttcc gccggtcgcc gcgaacgggc gcaccagggt cctgttgtgg
      481 cccgacacgt tcaacgacac gttcttcccg tcggtcctcc acgatgccgc gcgcgtgctc
      541 acccacgcag ggttcgccgt cgacattccg cgccggcggt tgtgctgcgg tcgcccgctc
      601 tacgagtacg gctgggtcaa gcaggcgcgc cacctgtggc agcgcacgct ccacgacctt
      661 gccgacgata tcgatgatgg cgttgccgtg gtcggtctcg agccgagctg cgtcgcctcg
      721 ttccgcgacg agctccccgc gctgtttccc gatgatccac gcgcggcccg cctcgcaaag
      781 cagacgtaca gcctcgcgga gtttctcgcg acgacgccgt acacgccgct gcgcgtgcgg
      841 ccgcaacacg tgctctatca ccggcattgc catcaggctg cggtgctcga gccggagcac
      901 gacattcgcc tgttgcgcgc cgccggtcat cggctcgagg tgctcgatag tggttgctgc
      961 ggcctcgccg gcgcgttcgg cttccagcgc gcgaagtatc caatctcggt cgcgctcgcc
     1021 gagcgtgtgc tgttgcccgc ggtgcgcgcg aatcctgacg ccgtcgtcgt cgccgatggc
     1081 ttcagctgcc gcgagcaatt actccagctc ggcggcgtgc gtgcgctgca cctcgcggag
     1141 ctgctcgccg acgtcccggc actggtccct gccgtcgtgc cgagcgatgc ggagctcgat
     1201 cgtatacgcg ccgcggcagc gcgcacgctg cgttagcgtt tgagctcgaa cacgagcagc
     1261 tcgccgagca tgtcgtgctc gcgcgttccg cactgcacca tgccgagctt gcgaatcacg
     1321 ccgagcgacg cgtggtgcca cggaaacgtc gtcgcgtgca ccgcgaggat gccggcctgg
     1381 tcgagcgccc agttcacgca cgcgcgcgtt gcttcggtcg cgaggccctg gctgcgcgag
     1441 ccgtcctcga tgccgtagcc aacctcggcg ataccgtcgg cggggcggcc cttgaacacg
     1501 acgctgccga cgacacgggg aggcccatcc agcggcagca cgagcctgtc gccccacaga
     1561 cgcgtatcgg gatcggcgcg gatcgcttcg agcgagaacg gaaacgcacg cgcgacgagc
     1621 tcttcgttcg gccacgcgtc gggaaatcgc gcgcacgccg gcaggtccaa cccgcagtcg
     1681 cggatgcacg catcgagcgc ggcttcagcg gccgcgcgat cgcgtcgcat cacgccttcg
     1741 atcatcgcga gcgtgatcgg caccaggcgc aggcgttcgg tagtcagcgc tggcatcagg
     1801 gccccgtcaa tccgaccccg acgagcagtg ccagctcttg cggtccgtcg ttgccgatga
     1861 gcccgtagcg aacatcgacg ttgaacatga aaccgccgac gtattgatcg agaccgacgg
     1921 tcgcgacgcc gcccgcgaac acgcgctgca tcaacggcgg taccggttcg ttcgtgcgta
     1981 gcagctcacc gccgacgtgt gcggtgaggt atcggctcgg cgtgtcccac ggccgaaccc
     2041 gcaggccgac ctgtagatcc atctgcaccg tgtgcagctc ggtgtcgatc tgcggtgcgg
     2101 tgccgttgta gagcgtgccg aacaacgtgc tccagcgcag cgaccagccc cacgatcgct
     2161 cggtggccgc cggttgccac gcggcctgca tgccgaattg atacatgccg tagccgagac
     2221 gcttcgcgtc ggcgccggtg cccgacagca cgccgaacag aaagccgatc gtacctggcg
     2281 gcagcggatc ggcagcgacc ggagacgcga gcgcgccaat aaccgccgca gcagcgagca
     2341 cgcgccgcat ggctagaact tcgacgtcgt cgcgaggaac gtcatcttgt cgttcgtcgc
     2401 gcggaggcga cgcgacagcg tacgcaccca gttccacaac agctcgtacg cgagatcgcg
     2461 atcgacgaac agcagatcct cgaggtcgcg gcgattgacg acgaacagcc ggcacttctc
     2521 gtggcacatc gcggtcgccg agcgcggggc atcgtcgatc agcgacatct cgccgaagta
     2581 gttgccgggc cggagcaccg cgagtgcttc ttcgcccatg ccgggcacga tgcggctgat
     2641 gcggaccgcg ccttcgacga tcaggtagaa cttgtcgccg ggctcgccct ccgaaaagat
     2701 cgtcgcgccg ttgcggtact gctcctcgac accgatgtcg acaacacggc gcaggtgacc
     2761 gagcggcaga ccgctgaaca agtcgatctg cgagaggatc tcgagcgtcg acttaccctt
     2821 gatatcgagc tcgctcggac gtggctcttc gcccatggac ggtcccaagc gtcccacaca
     2881 ccccccgaaa acgcaaaaac ggctcgggcc gaaacccgaa ccgtttacca gcgacgcgcg
     2941 accagaagga ctagccgacc ttcaggacga gcaagagcga gatgatgagc gcgtagatga
     3001 ccagcgactc gatcagagcg aggccgagaa tcatcgggcc gcggatttga ccggcggcgc
     3061 cagggttacg ggcgataccg tcgagggccg cagccgctgc gcgaccctgg ccgatgccac
     3121 cgccgagggc ggcgataccg atgccgagac cggccgcgag ggcgatcagg ccgaagttct
     3181 tcgccaggcc gtcggccttg accgccgccg cgtcgaacgc tgcgccggcc gcttcgggct
     3241 gcgcgaacgc aacggccgag atgccgagga tgaggaggac ggtgaggaac gaaatgatgt
     3301 tcttggtctt catgacaaat ctcctggggg tcgcgcgggg cgccggttag ttagcgtgtc
     3361 ttcgatgatg ggtaaagcag gaaagaagcg aaaaatcgat cagtgctctt cgtgttcgat
     3421 cgccatcgag atgtagacca tcgttagcgt gcagaacacg agtgcctgaa tgaggcacac
     3481 gagcaggccg agcagcatga acggcagcgg gacgaggatc ggcacgagcg cgaagaacga
     3541 catgacgacc ttgtggtcgg ccatcatgtt gccgagcaaa cgcaccgaca gcgagagcgg
     3601 gcgggcgaag tggctgatca tctcgatcgg gaagatcaac ggcgcgagcg ccggcgacgg
     3661 tcccatgaag tgcttgaagt agttgatcgg cccgtgctcc ttgatgccgt agtagtgcgt
     3721 caggaggaac accatcaccg cgatgccgac gttcgtcttg agcgtgtcgt tcggcgtcac
     3781 gaagcccggc acgaggccga tcaggttgcc gaacaggatg aacatccaga gcgcgccgat
     3841 cagcgggaag aacttcttgg cgttcgcctc gcccatcgcg ccttcgacca ttccgtagac
     3901 gctctcggcg gtgtactcga agaagttgcg caggttcatc ttgcgcggcg ggaccaggcc
     3961 tttgtccttg gacttcgtgc cgcgatagaa cgacatcgcg ccccagacgc agaacatgat
     4021 cacgagtgcc gtggtcagga cgtgcgtgag cgtccagccg gactcgtcga acatcatcca
     4081 cttccacttg cggccaagcg tgccttgcgc ccagtggttg aagtcctgcc accaggagaa
     4141 gcggttcaag tagtcgaacc aggtgccgtg ttcacccatg gtgctcgtcg tgctccgtcg
     4201 aaggcggctc tgcttgcgga cgcagagccg aataagtcgt gtcgagaaca atcgacacga
     4261 tgaacagcga ataaccgatc gtgaatgcga tcggatcgat cggtaggaac aagatcgcca
     4321 ccgcgaccgc gcccatgagg ccgatcatct ttggcagcat caagagcggc gcgttgccgg
     4381 tcttgccctt cgcggcgtcg ctcgtccact tgaagacgag cttgcgcaac acgaagaagt
     4441 tgaggcaggt cagcgcgaca ccgaccgcga tgccgagcgc gacatcttgc ggctgcgtca
     4501 ccgccgcggc gatcacgagg acgccgccga gtgcgtagtt catgcgctcg atgcgaagaa
     4561 ggctaggcgt tgccatgacc gcctcctttg tcttcgttcg cttgcgcttc ggcggcgcga
     4621 tcggcgcgcg cgacagcgcg gaacacaccg cggaatccgg cgatgagacc gagcaagagc
     4681 cacagcagca tcagccacgg ctgggtgccg agccacttgt ccatgtagta gccgacgagc
     4741 aggccaatcc cgacggacag tccgagctcg agcccgaccg agctcgcgtt gagcgcgttg
     4801 tacgcgcgct tcgcgttacg ggcggcgggg tctgcagcac ggctgctacg gcgatccaca
     4861 gaggttcgat gcgtgtttcg catgaacgaa tcctgcgaca tttcgccgct atggccagag
     4921 ggggccgcca tcgagcgggc cgtgggtacc acgcgagaat tccagcgtca atcagccgag
     4981 gcgatcgcct ccgacgacga tcaggacaaa ctgtcgcggg ccgccgcgat caccgaatcg
     5041 acgtcttctt cggtgtgtgc gagcgatacg aacatcgcct cgaactgcga cggcgggaga
     5101 aacacgcctt tgtctcgcat cttgcggaaa aacgcgccga acctcgcggt atccgaggtc
     5161 ttcgcggtcg cgtagtcggt gaccggaccg ttggcgaaga acagggtgac catcgagccg
     5221 atccggttca cgcaggccgg gacaccggcc gcttgcgccg cggcaagcag gccatcgccc
     5281 aggcgtttgc cgagctggtc gagccgctcg taattccccg gccggccgag gatgtggagc
     5341 gccttgagcc cggccgccat cgcgagcgga tttccgctca gggtgcccgc ctgatagacg
     5401 gggccgagtg gcgcgagctt ctgcatgatg tcggcgcgac cgccgaacgc cgcagcaggc
     5461 aagccgccgc ctacgatctt accgaggcac gtgatgtccg gcgtgatccc gaacaggccc
     5521 tgggcgccgc gataaccgac gcgatagccg gtgatcacct cgtcgaagat cagcagcgcg
     5581 ccgtgcttgg tggtgaggtc gcgcaggccc tgcaggtagc cgggctgcgg cagcacgaga
     5641 cccatgttgc cggcgatcgg ctcgatgatg atcgcggcga tctcgcccgg gttggcggca
     5701 aacgttgctt cgaccgcagc gagatcgttg tacggcacga ccagcgtgtc gcgcgctgcg
     5761 ccgtcgggca cgcccgcgga tcccggaatg ccgagcgtcg cgacgcccga gcccgcagac
     5821 accagcaagc aatcggcatg gccgtgatag ccgccgtcgg tcttgaggat cttgttgcgg
     5881 ccggtaaagc cgcgcgcgag gcgcagtgcc gtcatcgtcg cctcggtgcc cgatgacacc
     5941 gcgcgcacca tctccatcga cggatacgat tcgcgaatgc gctcggcgaa gcgcacttcg
     6001 atctcggtcg gcgcgccgaa cgtcgtgccc tgactcatcg cctcggcgat cgcgtcgagg
     6061 atctcgggct ggctgtggcc gaggatcaac gggccccagc tgccgatcag atcgatgtag
     6121 cggttgccgt cgacgtcggt gatgtacgcg ccgtcgcctt gcgcgacgaa cacgggatcg
     6181 acgccgaccg agcgacacgc tcgcaccggc gagttcacgc cgccggggat gacctgcttc
     6241 gcgcgctcga agatcgactg actgcgttgg atgcccatcg cgggcgaagg tagcgcgttt
     6301 tacggctgcg cgatcgctgt cacgcggggg ccgtcgctgc gagctgttct tgttcagccc
     6361 gcgcgcgcat cggctcgacg acgaatctgc ggatccgaga gcgcacgaac gggcgtgcga
     6421 gccatgcgag tgcgcgcaac ctgccgcgcg gcgcgatgtc ggcgatgaca gtgtaacgcg
     6481 tgccgccggg caccggctcg aagcgattga ggaaccgtgc ttggtagctc ggcttctttt
     6541 catcgagctg gatctcgcgc tctgacgtcg gggtgatcac gttgggcacg gtccccgcgc
     6601 tcgcgtgatc gacctccacc gtgatcgccg gtccatcggt acgcacccag cgcacgccgc
     6661 ggatcgtggc cgggaatagc cgtggccatg ccgtgtagtc catgtacagc gccatcagcc
     6721 gctcgggcgg cgcgtgcacg atgcgtgaga cttccgcatg gatgccgtgg gcacgcggga
     6781 tgtcgacgag cgtcgcgatg acgagcgcgc aaaacccgac gagcgtcagc acgaagccga
     6841 tgaggtggcc gtactcccag cgcggacgca acgtcaccca ggcctcgacc gccgatggac
     6901 cggcctcggc gatctgctgg tttaccggca cgacgagcac gagccatgac acgaacgaag
     6961 cgaggactgc gagcgccgcg aggagcgtcc agtgcgccga tcgctgtcgg cgaacccgcc
     7021 acgccagggt cactacggcg atcaacgcga cgatcgtgta agcgccaccg acgatcgcga
     7081 aataccgata cagcgtaccg ttgaccgcgg tgtagagctc gtagctgtac gcgagcttcg
     7141 gagccatctc gagcacgtgc gccgacgtca tggtcagcgc gagcgcggtg gcgacgagtg
     7201 cgatgaagcg gtggaacaac atccaccatc acttggtgcg agcgacgtgc ctacggctgc
     7261 gccagctggg ccgtgccttt gccgacgatc tcttgctcgt aatactgctc cggcttcacg
     7321 ccgcgcttct ccgccgcggt cgctttcgca agctcctcgt cgatcagcgg cgaaaacttg
     7381 tcgatcggta gcgcgcccgc gacgtagcgg ccgttgatga agaacgtcgg cgtcgcgccg
     7441 acaccgagcc gcgtcatggt cgccatctcg tcgcgcactt cctggacaca cacgcccgcg
     7501 acatcttgtg cgtagcgatt catgtcgagc ttcagctgct tcgcgagcgc cgtcatgtgc
     7561 gccggcgaaa acgcgtcgct gttgttcgcg cgctgatcga acgcctgcgt ccagatcagc
     7621 tgcgccatct cgcgccactt gccttgcttc tgcgcaccgc acgcggcctc ggcggcgagc
     7681 ttcgcggtct tcggatgcac gacgtacggc ttgtatgcga cgcgcagatc cgggccgtac
     7741 ttctggcgca gctgctcgac ggtgtcccac gccttgcggc agaacggaca gtcgaagtcc
     7801 atcgccatca ccagcgtgac cttcgccttg ggcgagccgt agaccgggct cttgccgagc
     7861 ggcaccgagt acgtgaccgc gggatcgggc tcgcgccgct gcccgcgcgc cggcttcgct
     7921 tgcttcaccg cgtcgaggag cgtgtcgagc tggtcgtgca ggtcggcgag ctggccggcg
     7981 agggcagtga gctcggtagt gcctggctgc gcgcggagct cggccagcgc cttgtcctgg
     8041 gcgtcgagtc gttgctcgat ctgcgcgagc cgctgcaatg tctgattatc gcgcttttcg
     8101 cggtaacagc ttgcgaggag catcacgatc aacaaagcgg cgatcggttt catcgggggc
     8161 aagatacaat gaggacgtgc gctggggact tgtcgggctc gtgctgttcg tgtgcgcgtg
     8221 cgactcgcgg tacggcgcgt atttcgtgat caagagcgac gtcgaattcg atgccgtcga
     8281 ggtctacttc ggcactccga tcgactcgtc ggggccgggc agcggcgatt cgttcgcgac
     8341 gcctacgttc ggtgcgcagc gcgggacgct gttcgatcgt acgtttgccg acgcagatgt
     8401 gttgacggtc ggcgcgacga ccgaaaccac gttctacgta ccgccgaccg aagcgaatca
     8461 gcagctcggc gcctacgttg cggcagtcgc gttgtcgggc ggtcatcccg tcggcatcgc
     8521 cgagtacttc gggttcgcgg taccgagcga tgtcgtgcac gagtaccagc tgaagctggc
     8581 gccgtggaat ccgcaggaag ccgagcgctg gggcgatacg ccgggatgtc tggtgtggaa
     8641 gcacgcgcgc gacaacggca ctgggtttgt cgcggtggtg cacggtgacg atcgcgattg
     8701 cgacacgttg gtgagaaacg ccgactgcaa cgatctgtgc agcgcgggat cgccagcatg
     8761 cgcggcggat acttcgttct gtggcggcgc gaccggcgtt tgtgcgctcg gctgttcggc
     8821 gaacggcgtg tgctcgccgt cgctgtgctt gccgccggcg acgtgcgaca cgacgtgtgc
     8881 tgcactcggg aacgtcacgg acaggctcgc gtgcggcgca gccaagacga gtaaccatgc
     8941 cgagatcttc gtcgagcgca acgacacgct gcagatgtgc gcgatggagt ttctgttcag
     9001 ccccggtgtg ccgtgcgtga tgccgaagat cgaggcgaag gtgccggccg acttgacggg
     9061 cttcacgtac gcaatctcgt cggtccccgg caacacgatg atgtgcaacc tcggcgtcat
     9121 tcaggacccg atgacaccgc tcgatgattt cgacgagcat cacctgctga tctcgttcgc
     9181 gccgccgacg aacatcggtc cgcggccaac ggttgttatc ggcatccaag caaaaccaaa
     9241 ttcggtcgcg tgcgcgaacc agccgtatta cctggtccag atgccgactc ctccgcaggt
     9301 ctacgcctgc ccgtagccgc gccgaaaacg taggcgattg gagcaatcca gacggccagg
     9361 aagtcgccgg aataattgcg ttatcaggtg gcttcagaca ttgaggaatg acgctgggcg
     9421 agctatacga atgcactgcc gatggctgac gtaagcaccc ccaacatcac gccgatctcg
     9481 atc
//
LOCUS       DASUFX010000077         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1958130, whole genome shotgun sequence.
ACCESSION   DASUFX010000077 DASUFX010000000
VERSION     DASUFX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958130"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFV99_02945"
     CDS             <1..510
                     /locus_tag="VFV99_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX5058285.1"
                     /translation="AAETVGAGSRSVPAHSAQARLAPYAHRATDVVMTYQRGLLPLSS
                     LGAANSQITARAGQLRRRLRGAGYQLHGNLLEVSPAATIAALCGPRVARGYKRDADPW
                     VTRATILERFTDLSFAPQSRMAREDVLRNDHCFDAVISAYTAYLWARDGWELPTGVFA
                     DDGWIFSPP"
     gene            605..1651
                     /locus_tag="VFV99_02950"
     CDS             605..1651
                     /locus_tag="VFV99_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5058286.1"
                     /translation="MRGLSGTSVRRLAAPRVHAHRAHMKTTNEVSPPRCDVCAPSNNA
                     GTHAVVRPQKIARPSTDGMVRLDGGSFWMGSDGRDDAPADDEEGPSHRVRVNPFWIDR
                     TCVSNAEFSAFVADTDYRTEAENAGWSFVFYQLLPDDFPPTPPVPSAPWWRHVRGASW
                     RHPEGPHSSLAGRMDHPVVHVTWNDALAYAQWNGKRLPTEAEWEYAARGGLSKRVYPW
                     GDELAPDDEERCNVWQGHFAIDRMSRGDCAGTAPVASFLPNGYGLYNMAGNVWEWCGD
                     WFSPTYYLHSRHVDPRGPLSGHERVIRGGSYLCHDAYCHRYRVAARSHSTPDRATGHI
                     GFRCARDAGSISSG"
     gene            complement(1648..2463)
                     /locus_tag="VFV99_02955"
     CDS             complement(1648..2463)
                     /locus_tag="VFV99_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5058287.1"
                     /translation="MAVPRSNARVADGVRQMCGRGELVRLLYRFGRQGASGVLTITTA
                     GSHRAEIFVLRRGAIMLAEGDLAKRALVGRLVRLVALDRLEVVFEGGVTAYPPGGQNQ
                     LPLATWARTHLEQQLDSTLAEVLLRELAGVRLSIRVELAPEPPTCDEADRRMLLALAQ
                     PRRLDQIWSMARTPRFRLLSFIHFLRAVDALDVEGVVADRSARTRSIDPRREAARRLL
                     GLDDDADLESIKRAYRRLARELHPDLQPDAEGDARRTLERRFAEVTAAYEALI"
     gene            complement(2577..2819)
                     /gene="rpoZ"
                     /locus_tag="VFV99_02960"
     CDS             complement(2577..2819)
                     /gene="rpoZ"
                     /locus_tag="VFV99_02960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5058288.1"
                     /translation="MARVTVEDCLDKVPNRFALVILAAERARQLSRGATPGVDCDNKP
                     AVTALREIAAGLVKFREDVRNTVTEFIVERRKAGFM"
     gene            2958..3665
                     /locus_tag="VFV99_02965"
     CDS             2958..3665
                     /locus_tag="VFV99_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HEX5058289.1"
                     /translation="MPVRPLVPAANRGPVRIDLDWDALEIAVERNSPDTDSYLDLSTG
                     RVLTITSGDPEATLNRQQVSENIRNYLRVEPASSREQYRWMEKFVGSVTDEPLRERLI
                     ISIDGKGAFRRFKDVLLAYPAERERWFSYRAELLHWHIQQWLEQHDIHPTNEPPWGAV
                     AAPTELPTVGRPVVHGTEAPGEALRRQARDLIETIPAIELPTAIAFLEFLKARSAEAL
                     AKEHRENLAIQDDDPIK"
     gene            3662..4009
                     /locus_tag="VFV99_02970"
     CDS             3662..4009
                     /locus_tag="VFV99_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058290.1"
                     /translation="MSSIEELATALVQQAPDLDPRTIAELRDQLWAVGSPLALAIARI
                     YELVDIDVVDPAIALPALAEACATLVAGIEGRVGERELEAARYQIDTLEPVPDKPPRV
                     AAPDVPITKLRKR"
     gene            4042..4218
                     /locus_tag="VFV99_02975"
     CDS             4042..4218
                     /locus_tag="VFV99_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5058291.1"
                     /translation="MSEATKGIDTVVTLTLTRTEGGTEVKLTHANVPDDDMGRMHSEG
                     WRSILEAIAKHLEK"
     gene            complement(4219..>4609)
                     /locus_tag="VFV99_02980"
     CDS             complement(4219..>4609)
                     /locus_tag="VFV99_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058292.1"
                     /translation="EKPEHIVGDVFKVLARFVERGRKFDMVVVDPPAFASAAARGGKP
                     WSAMRDYAELITAALDVLEPGGLLAAASSTHKMSSEEFERALAEGAMAAGTRLQIIDR
                     RPLPPDFPTAPAFPEANYLKFVVAVRG"
BASE COUNT          801 a         1567 c         1514 g          727 t
ORIGIN      
        1 gccgccgaga cggtcggcgc cggctcgcgt tcggttcccg cgcacagcgc gcaggcgcga
       61 ctcgcgccct atgcacaccg cgcgaccgat gtcgtgatga cgtaccagcg cggcctgttg
      121 ccactgtcgt cgctcggtgc cgcgaacagt cagatcacgg cgcgcgccgg tcagctgcgg
      181 cgccgcttgc gaggtgccgg ttatcaactg cacggcaacc tgctcgaggt ctcgcccgct
      241 gccacgatcg ccgcgctgtg cggacctcgc gtcgctcgcg gctacaagcg cgacgccgac
      301 ccgtgggtca cgcgcgcgac gatcctcgag cgcttcacgg atctgtcgtt cgcgccgcag
      361 agtcggatgg cgcgcgaaga tgtgctgcgc aacgatcact gcttcgacgc ggtgatctcc
      421 gcgtacaccg cgtatctgtg ggcgcgcgac ggttgggagc ttcccaccgg tgtgttcgct
      481 gacgacggct ggattttctc gccgccctga gacgatcagg tctcgcgcat cttggcgtac
      541 gcaacgctcc cgcgcgcgct cgcgcgcagc gcacgtcgtg gaacccgccg tgatcgtgca
      601 gcgcgtgcgc ggcctttcgg gcacgtcggt tcggcgcctc gcggcaccgc gcgtgcacgc
      661 gcatcgcgcg cacatgaaga cgacaaacga ggtcagtccg ccgcgttgtg atgtgtgtgc
      721 gccttcaaat aacgccggca cacacgcggt cgtgcggccg caaaagatcg cgcggccttc
      781 gaccgacggc atggtgcgac tcgacggtgg atcgttctgg atgggcagcg atgggcgcga
      841 cgacgcgccg gccgacgacg aagaaggacc gagtcatcgc gtccgcgtga atccgttctg
      901 gatcgatcgc acgtgtgtgt cgaacgccga gttctccgcg ttcgtcgccg ataccgacta
      961 ccgcaccgaa gcggagaacg cgggctggtc gttcgtgttt tatcagctgc tgcccgacga
     1021 ctttccgcca acacctccgg tgccgagcgc gccatggtgg cgacacgtgc gtggcgcgag
     1081 ttggcgacat cccgaaggac cgcactcgtc actcgcgggc cgcatggatc atccagtcgt
     1141 ccacgtaacg tggaatgacg cgctcgcgta cgcgcaatgg aatggcaagc gattgccgac
     1201 cgaagccgag tgggagtacg cggcacgcgg cgggctatcg aagcgcgtct acccgtgggg
     1261 ggacgagctc gcgcccgacg acgaagaacg ctgcaacgtc tggcagggcc acttcgcgat
     1321 cgatcgcatg agccgcggcg attgtgctgg caccgcaccg gtcgcgagct ttctgcccaa
     1381 cggctatggc ctctacaaca tggccggcaa cgtctgggag tggtgcggcg attggttttc
     1441 gccgacgtac tatctgcaca gccgccatgt cgatccacgc ggaccactca gcggacacga
     1501 gcgcgtgatt cgcggcggct cgtacttgtg ccacgacgcg tactgccatc gctatcgcgt
     1561 cgctgctcgc tcgcactcga cgcccgatcg cgcgacgggc cacatcggct ttcgctgcgc
     1621 gcgcgacgcc ggctcgatca gctcgggcta gatcagcgct tcgtacgcgg cggtgacttc
     1681 ggcgaaccgg cgctcgagcg tgcggcgcgc atcgccttct gcatcgggct gcagatcggg
     1741 atgaagctcg cgcgcgagtc tgcgatatgc gcgcttgatc gattcgaggt cggcatcgtc
     1801 atcgaggccg agcaagcgac gcgctgcctc gcggcgcgga tcgatcgaac gggtccgcgc
     1861 cgaacggtcg gcgaccacgc cttcgacatc gagcgcatcg accgcgcgca agaagtgaat
     1921 gaacgagagc agacggaagc gcggcgtgcg cgccatcgac cagatctgat cgagccggcg
     1981 cggctgcgcg agcgcgagca acatgcggcg atccgcttcg tcgcaggtcg gcggctcggg
     2041 cgcgagctcg acgcgaatcg acagccgaac gcccgcgagc tcgcgcagca atacttcagc
     2101 gagcgtgctg tcgagctgtt gctcgagatg ggtgcgcgcc caggtcgcga gagggagctg
     2161 gttctggccg ccgggtggat atgcggtgac gccgccctcg aacacgactt cgagtcgatc
     2221 gagcgcgacg agccgaacga gccggccgac cagcgcgcgc tttgcgaggt cgccttccgc
     2281 gagcatgatc gcgccgcggc gcagcacgaa gatctccgcg cgatgcgagc ccgcggtcgt
     2341 gatcgtcagg acaccgctcg ctccctggcg gccgaaccga tacagcaggc gcacgagctc
     2401 gccacgacca cacatctgac gcacgccatc cgcgacacgc gcattcgaac gaggaaccgc
     2461 cacggaccca tcgttgatgc gtcgcgacgt cggggcaatt tttcagccga cccgagctag
     2521 cgaggcgact gttcagcgaa tctgcgcgtg caggagacga agatcgtctt gcgcgatcac
     2581 atgaagccag ctttgcggcg ctccacgatg aactcggtca ccgtgttgcg cacgtcctcg
     2641 cggaacttca cgaggccagc agcgatctcg cgcagggccg taaccgcagg cttgttatcg
     2701 cagtcgacac cgggagtcgc gccgcgcgag agctgccgcg cacgctcagc ggccaagatg
     2761 accagagcga agcggttggg gactttgtcc aagcagtctt cgacggtaac gcgggccatg
     2821 tgagcggcgg aacttacccc agcgcgctgg catgtcaagg gcacgcttgc cagacgttcg
     2881 actaccaact accctcatga aagcggccat gatcctaccg gcaagtaggg tcgatctccc
     2941 ttgggcgcct ttaacttatg ccggttcgac ccctcgtgcc tgccgcgaat cgcggccctg
     3001 ttcgcatcga cctcgattgg gacgccctcg agatcgccgt cgaacgcaat tcgccggaca
     3061 cggacagcta cctcgacctg agcacaggcc gggttctgac gatcaccagc ggcgaccctg
     3121 aagcgacgct caaccgtcag caggtttcgg agaacatccg aaattacctg cgcgtcgagc
     3181 ccgcctcgtc ccgcgagcag taccgctgga tggagaagtt cgtcggctcg gtgaccgacg
     3241 aaccgctgcg cgagcggctc atcatctcga tcgacggcaa gggcgcgttc cgccgcttca
     3301 aggacgtgct gctcgcctac cccgccgagc gcgagcgctg gttctcgtat cgcgccgagc
     3361 tgctccactg gcacatccag cagtggctcg agcaacacga catccatccg acgaacgagc
     3421 cgccgtgggg cgccgtcgca gcgccgaccg agctgccgac cgtcggccgg ccggtcgtcc
     3481 atggcacgga agcgcccggc gaggcattgc gccgtcaggc gcgcgacctg atcgaaacga
     3541 tcccggcgat cgagctgccg accgcgatcg cgttcctcga gttcttgaag gcgcgcagcg
     3601 ccgaagcgct ggccaaagag catcgcgaga acctcgcgat ccaagacgac gatccgatca
     3661 agtgagctcg atcgaagagc tggcgacagc gttggtgcag caagcacccg acctcgatcc
     3721 gcgcacgatc gccgagctgc gcgatcagct gtgggccgtc ggttcgccgc tggcactcgc
     3781 gatcgcgcgc atctacgagc tggtcgacat cgacgtcgtc gatcctgcga tcgccctgcc
     3841 tgcgcttgcc gaggcttgcg cgacgctcgt cgccggcatc gaaggccgcg tcggtgagcg
     3901 cgagctcgag gcggcgcgct accagatcga cacgctcgag ccggtgcccg acaagccgcc
     3961 gcgcgtcgcc gcgccagacg tgccgatcac gaagctgcgc aagcgctgac agatcaaaca
     4021 cgatccgccg tgcacacgtg gatgagcgaa gcgacgaaag gcatcgacac cgtcgtcacc
     4081 ctcacgctca cgcgcacgga gggcggcacc gaggtcaagc tcacgcacgc gaacgtgccc
     4141 gacgacgaca tgggccgcat gcatagcgaa ggctggcgct cgatcctcga ggcgatcgca
     4201 aagcacctcg agaagtgatc agccgcggac tgcgacgacg aactttagat agttcgcttc
     4261 cgggaacgcc ggtgccgtcg ggaagtcggg tggcagcggt cgccgatcga tgatctgcag
     4321 ccgcgtgccc gccgccatcg cgccttcggc cagcgcgcgc tcgaattctt ccgatgacat
     4381 cttgtgcgtc gacgatgccg cagcgagtaa cccaccaggc tcgagcacgt cgagcgctgc
     4441 cgtgatcagc tccgcgtagt cgcgcatcgc gctccacggc ttgccgccgc gtgctgctgc
     4501 gctggcgaat gcgggcggat cgacgacgac catgtcgaac ttgcggccac gctcgacgaa
     4561 gcgcgcgagg actttgaaca cgtcgccaac gatatgctcc ggcttctcg
//
LOCUS       DASUFX010000078        20263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_12699,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000078 DASUFX010000000
VERSION     DASUFX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12699"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VFV99_02985"
     CDS             <1..1338
                     /locus_tag="VFV99_02985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5058293.1"
                     /translation="QREVSADRAFDDNSAVWLLGAPADTPAPPLARDLDVDVAIIGGG
                     FTGVATAYRLSQRFPKLGIALLEAKQLANGASGRNGGLVLNGITVKDVDADLMVREHA
                     ITKRAIDDIEALIREHSLPVRFRRTGCVHVATTAEAAEHAHAMTEQLVARGLPLRFLR
                     GAELDAVLPVRGAHGAVFDPTEAMLVGVDLIRAMRPLLVAQGVQIYEGTRVTRVREGA
                     TIELATPGGVVRAKSVVIATSGYTPRLGYFTTGLLPVISHVIATDPLPADLLASVGLA
                     KIAGFFDDSPRLAYCSVDDAGRVVFGGGTTAAYAYRFGNATAYVPQRDDAAERALRAS
                     LVGYFPQLADVPIRHRWSGPLDLTLVRHCAMGVLGEHRNIYYAVGFSGHGITLANLAG
                     RVLTDLYAGEHDRWKDCAFYMRKPSGIPPEPFRWIGYHLYTKLTGKSPWKRPE"
     gene            complement(1341..3650)
                     /locus_tag="VFV99_02990"
     CDS             complement(1341..3650)
                     /locus_tag="VFV99_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5058294.1"
                     /translation="MWILLGLSALPSCATSGEEETAKYDDELVLTAATPINDEGLLAF
                     DTAVFPENPTTLLEQGDFHGYKFNGKAGGKITIAMSGERCGQPDTLVHLFGPENVDGD
                     RGADLIRNDDFFNGIACAKDSEIVDFTLPVDGEYLIVATSFAQQGGGHYRLTLICTNN
                     ACAPSGEMTFQSSRIAQTDIDHGAFTPDELFEVGDFLFENIYGVEDGLGNALTGAPAG
                     TNPRPNFRTVHQGGFGAPEAQSCITCHNVGGDDGAGDLNHNIFQNGDGVNRSSGLVRN
                     PPIVLGLGLRQQIGIEMTNELQAQLAAAKSAAASSATSVTRSLSSKGVSFGSVVVTPQ
                     GFVDITNLQGVDPDLVVKPFGWKGREATLRRFVEGGFRVHFGMQSAPSIAKHCAAPDT
                     ATFGTGSNCRDPDNDGVTDEITEGQLTAMATYMGLRETPVRVPPADTNGRARATAGEQ
                     LFGSVGCGDCHVAQMRLSSTTHVEPADTTGGAGITFDLATDMKSPHPARNSDGSTTVE
                     LWSDFKRHDMGSALADSKPFKSIAPAQFITTPLWGVATSPPYMHDGRAPTLRAAILAH
                     AGEALGARTAFAALSSDDQQKVLEFLGTLGRAPEDGVARTVEGVDVSRFTIRQANAAA
                     NFQLPAGTVVPHGGYVIVARDATRAELEAFYGRSLGSNVVYINSGAKFPQINGSETFS
                     LLNAQGTLVEGPMAQLPSAASQIFQRVSGAAPAGTASSWTVRPASPQNATPGSGQAST
                     GNGRVYLSELADAAGTGNFKFEFVELFVE"
     gene            complement(3683..4969)
                     /locus_tag="VFV99_02995"
     CDS             complement(3683..4969)
                     /locus_tag="VFV99_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5058295.1"
                     /translation="MRVSLFILVLATSACGTDVRPPGGIVETDEPTFEAIRSDVFATS
                     CGFGSCHGGPTPAAHLDLRSDDMCSALVGHTSCLFPNKMLVVPGKPELSFLLDKLTGT
                     HLDGAPATGCADSNEPMPFGAPRLDAEKIARIEAWIRAGARCERMGDYNDMPPDAGMT
                     VDAPVASIAAAATTIRAGEHTRITITLALPAPPGGQSIDLDVDNATVLGVPSAYHVDA
                     GVSSVDIDVIGKRPAHAVRITATAGSISRSTSIAVTGLQLSEIEFDPISTDDGNEWIE
                     LANTTDVAIDLSAYSLGSGRTGYTYSMAQLAGMIPAHGCFVVGGPTSTTANGGPVYGQ
                     TYNFSPDLINGSGTNGQAAGFALFDVPITQLSPSTIPLDAVLCGLNNGAGLLDASGTA
                     ATPRCPDVAAGHSVSRTSSTTWVDQPTPTPNTCSPQ"
     gene            complement(4969..6570)
                     /locus_tag="VFV99_03000"
     CDS             complement(4969..6570)
                     /locus_tag="VFV99_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5058296.1"
                     /translation="MSLSFLARCFVAAVAVSTLGCHADSGETFTRTLRWHQEPGSEIQ
                     DCHVFKLDNADPIEVERITVQFAAGSHHVHIYKSDTPVDDDVSDCWQGIDWTRWHLVL
                     GAQTAPIDWSLPAGRTVPLAPHQQLLVQVHWLNTTNTPIDGSVELAFHTTDHSDAHVG
                     VVFGINKQTAMQPHERKVIRQWCPLPSHTELLGLMGHFHGLGTRYSIDTRPEGAADGS
                     PVYDALDEQTFQFKVFDPPPPVPDGDGLQFECDFFNHTDMPITWGADTKRSEHCNMVA
                     YYYPAEELSTFCIVEAAEVRALEGPKTPVPLDALATYRIVLSEPAGSDGARVRIATID
                     PMALEVPDTVEIAAGQSEATFTARARRPHRVTVTATLGGTSKTAVTTIGGLVLSEVYT
                     GTAGDTTNQWVEIANLSNAAIDLSTYSLGAGRDDYTAMRKPLDVVVPAKGCVVVNGFS
                     PALGVAGSDASGIALFDNSADQITHTTLPYDALVYGGDNNSLLDPTGDLADVVAAAPA
                     SGTYVRTSERWTTQPVGTPGICEVH"
     gene            6891..7445
                     /locus_tag="VFV99_03005"
     CDS             6891..7445
                     /locus_tag="VFV99_03005"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5058297.1"
                     /translation="MITVDAILGSVERILEQDGPSALTTNRIAEVAGVSIGSLYQYFP
                     NKEALVGALQDKYADDTLSRVRSVLVDAERMPINLVIARVAEAVLSAQHDQRPIHRWL
                     NEWRSVTGSHERYRRALDEHVELIADFLSRRPDVHLSDHHAAAFVLVHAIEGIIEAAS
                     ERGAEVDATAIAMQTIRMVTVFIA"
     gene            complement(7447..9204)
                     /locus_tag="VFV99_03010"
     CDS             complement(7447..9204)
                     /locus_tag="VFV99_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5058298.1"
                     /translation="MTTVAIVYHSVSGRTRALADAVARGAGSVADTQTQVFSVANVDH
                     AALQAADAIVFGCPTYMGSASGAFKSFMDGTSAIWALQAWRDKLAAAFTHSAAPSGDK
                     LATLMQLSVFAAQHGMVWVGLGLPPTYAAPEPVATDTNRLGSHLGAMAQTPPGRTLDE
                     SDVRTAEHLGRRVAQAAQRWKLAETPRSEIARHAATNTWVFPKASRPALPAPLSRINL
                     REIVARPERFEHHLIVCATIGRVQLEIVTASEPLYFGHVNLSDEYAIPLPCGDDLVDR
                     FPLRTFLSDAASGADVGRYNHRNSDLVLHPIGYLHWPGRLRPPYEPFELPPGVRRCGI
                     SLVFCANQHTPSSAPRRGAPSGRDGDIKPYATPAPPMMIAAVSGEPGVVAKIGTASFE
                     LVDRPTAIAPARGGWVVIVEAAPTSAHAACDLIRIPAGASLDTTGVVRAFVLSDTDAS
                     PDEAPPSWRGLPPSPFAPFEDAARGNLPLHHGELAVEHASPTEVTIRVRDTVTTVPRY
                     WLARMLFRVALHGLRLGYLETYGGLFIDDDAAGRDFQLGIRTPSGKTAITVPASDALA
                     MIERLYRAVAPDGYRERLL"
     gene            complement(9201..10475)
                     /locus_tag="VFV99_03015"
     CDS             complement(9201..10475)
                     /locus_tag="VFV99_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058299.1"
                     /translation="MMLVSKTYWAGIPFWFRKPPEDAVVESLALRAHDLRQFRAHYWT
                     SKRHAAPKVGVVVMHPRVDFSHHYSIPRLVAAGFGVLAANTRHAGNDTMAEHEEMVLD
                     MAACVRHLLEKRGVDKVVLLGNCGGASLSAYYQAQATLPAAERITRSPAGNPTHFESA
                     PLTPAHAIIYTAPHRGQGKVLMEAIDPSVTDENDPLSVDPALDMYDARNGFAEPPAWS
                     EYSDAFLATYRAAQRARVARLDAKARELLQRAADASAAATAPDFASRSADERRDIMRR
                     RVADPMMVVHRTNANPAFVDKRIDPSGRDYGSLLSERPDLMNYSSLGLARTCTPRAWL
                     STWSGLSSKADLVANVANITAPTLLVHAGRDREIFPADIAAIDKAIVARDKRVTTVEQ
                     ARHYFEPELGEKATPHVEALMDIVVPWIQERT"
     gene            complement(10487..12739)
                     /locus_tag="VFV99_03020"
     CDS             complement(10487..12739)
                     /locus_tag="VFV99_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058300.1"
                     /translation="MRAGCILLLVVAAGCLDPRTQHCADGSVCGPGLFCATAGGATVC
                     VTDEQFTACNGLASGARCVTTDIFAGTCIGGACISSGCGNGITDDGEACDDGNLVSGD
                     GCSADCRSAEVCGNSITDTDEECDCGFDPDDPTEHNPACPGPNSDTSGPCSMDCHLRC
                     GDGVVSADEGCDPGITATVSCAGTVFDRGLTTCSASCQPVIKPETCRYIGFRTRVKQP
                     VMSSTSTPLVTKLAGVTESNGFYIAENRANTYTNYMMVGNPYISSTPFGGIWAANMTT
                     AIAVGGMGKAVHYSGTSWVVRTTGTTVDLRDVWGRSASDVYAVGDSTVLHYNGTIWSA
                     LSPPAGNYRAVSGDATHVYVVGDQGKVAIYDGTQWTTEDPGTTVDLVSVWAAGGMVVA
                     VGDAATIRMRASNGTWSQGRTATTVNLRSVWGSTTDGFFAVGERGTVLFYDGAVWRPL
                     ALGRGVTGSPAQTLFSVTGIEGVVVGIVGSDDVMGYEGAAWSPTIQPTSETLYSIWGT
                     APDNIYAVGRNGTILHHDGLSWTQQPSPTSFDLHAIQGTAANDIYAAGDSLALLHYNG
                     SQWTSVGGGGGNFTSLLATGTNRVLGASTSGIYEYDGTTFSRVTITTGRAIWGASATD
                     VWVAGAAFAHFDGTSWTTTTTMTDATTLIGTSANDIYAVGKSPLHYDGATWSAGPFKD
                     DRLIAACLTPSNRLFGVGQQGALRYWNGTAIEPMISRTTADLSAVFMTGHVLVMAGAD
                     GTLDTMVFHR"
     gene            12766..15480
                     /locus_tag="VFV99_03025"
     CDS             12766..15480
                     /locus_tag="VFV99_03025"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058301.1"
                     /translation="MGENDATRPPSGQARGAAGMRARVGQYELIREIGRGGMGTVYLA
                     RDTRLARRVAIKFIAYSSAAMNERFLVEARATAQCNHENIVVIHEVDELEGQPYMVLE
                     YLEGKSLRELLDGTPMSPGRAIELIVPVARALACAHERSIVHRDLKPENIFVTNAGQV
                     KVLDFGIAKVHGEPDRDGDDEGTGEHTQASGLVGTLAYMAPEQWGADDVDHRCDLWAL
                     GIVLYELLAGRHPIQPTTKDRLMSAAMRPDEPMPALGSPSIDAELQRVVARCLAKRKA
                     GRFATAAELVAALEPLLPRRRAVGAAEIEPYPGLTAFREEDADLFHGRRSEVARLSAL
                     LRDRPLVGVVGASGAGKSSLVRAGLIPELKSREAWEALITRPGRHPLDALAVTVAGEA
                     DSASLATKLANEPGALGVRLRERARTRGGKILLFVDQFEELYTLADDRARAAYLACLR
                     GAADDPLSPTRVVVSLRSDFLDRVATDAQFVEALSPGMQFVVGADRDGLRAALVEPAQ
                     YAGYRFETTDMIETMVDELATTAGALPLLQFAASSLWQQRDRGQRLLTRAAYDRIGGI
                     AGALASHADAVIASMPPATRDIARQVLVRLVTPERTRDVVEVAELHELRDARAVIDHL
                     AAARLVAIRSDQGPAVVELVHESLIERWPALRGWLDQTQGDTAFLARVRTAAKQWDDA
                     ARSPGLLWHGDPEQDARRWLRDYAGELGSRERAYLDAVFQQADRAARRRRFAIGGTIA
                     MLGLLVVAAIVALVFIRDAQTEAEREASRAHDEANKASKANRDLSDALANLQRETAAR
                     EAARQAEAAATQQVNAARVDLERVNGDLRIALTRAEAESNRAKSESERAKRESEHAQT
                     ESDRAKSEAERAELARRTAEEERARAEDLARKERERAERRGKLSTTLP"
     gene            15591..15896
                     /locus_tag="VFV99_03030"
     CDS             15591..15896
                     /locus_tag="VFV99_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058302.1"
                     /translation="MMTKLLALVAAVSLAACVDDTSAPTDESAGSIDVQKDEQVVEPV
                     GPTDLCAVLPTDEADPCSHLCDEEGGGINAFIPEHTCVLFVCHLTDGSTYHAGGCVP"
     gene            15926..16915
                     /locus_tag="VFV99_03035"
     CDS             15926..16915
                     /locus_tag="VFV99_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058303.1"
                     /translation="MTRGWGLVRAVLVLCTLSLAHPASADTRPWAVGVSDADQARAKE
                     LFEQGNKLLDQSAFGDALKLYEEAITIWDHPAIRYNIAVAQMNLQRPIEAYRELERAL
                     RFGAAALEADVYQQAVTYQRIIGGQIVHLTIDCVDAETRITLDNNEQRIPCPGSSTVL
                     ITPGGHKLVASKAGSLTKTINIAPRPGDAPRELIDLMTLTEATVTRHRWPRWKPWVVI
                     AAGVAVAGVGLGFELQAAATYRSYDTAVDTLCGTMPCSNLPPVVADAYDDGRRQNRIA
                     VGLFAAGGTTFAAGAVLLWLNRARSERIGYDRASIVTAKIGAHASLVAAQWTF"
     gene            17047..17793
                     /locus_tag="VFV99_03040"
     CDS             17047..17793
                     /locus_tag="VFV99_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058304.1"
                     /translation="MLATLFLFAGCEKDLLENVHDAGADADLPVVDAPVGRCKAPTTD
                     PPPAAQYTLFVNFDGVTLGVCSSPDSRANCSNLIQTDTTFPAFLDGNPARDSFIASIL
                     FPVRKALAPYSIDVVTTRPASGNYYMMVVGGTPALIGAPSGVGGIAPASCNPADLRNG
                     VSLIFDRGQAPTTSDYASSILSDMGVFAAIPVTNSPVTDCLCRVNGCDFKGGLCTYGV
                     NSTVNPDPQFNCGLTAVDEPAMLQAVLGCR"
     gene            17805..18542
                     /locus_tag="VFV99_03045"
     CDS             17805..18542
                     /locus_tag="VFV99_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058305.1"
                     /translation="MRKSLVFVVLLAAGCEQDLLADVHDAGPDGPPDAAHCTAPLTDA
                     HAPAMHTLYINFDGVTLTHCNKANAPGNCSDIVQGDTTFPPFMDGLPARDGYITWIVD
                     AVKQSLAPYSIDIVTTRPAPGTSYYMTVFGGTPALVGADDTVGGISPFTCSAANRNLV
                     SMIFDFGENRTTYDYASSVLSDLAMMAGLPGTTGPNNDCACRSCAYDQYQLCTYGVDS
                     PTIPDEHSCGLTTADEPALLRASLGCR"
     gene            18552..19298
                     /locus_tag="VFV99_03050"
     CDS             18552..19298
                     /locus_tag="VFV99_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058306.1"
                     /translation="MRSTLFVLLIIGTGCEKDLLADMHDATVADAQQVEVDGGRCMSP
                     MTDAHPAAMHTLFVNFDGVLLTPCGTADARLNCTGLVSTETMIPPFMDGNPARDAFIA
                     TILYPARKALARYSLDIVTTRPTSGDYYMMIYGGTPQLLGLPAANVGIAPFSCMPDNH
                     NNISFTFDRGQAPTNIDYADGILSDMGVFAGIPGTTVPGDCLCRSCQFPTDTVCTYGV
                     DVPVSTDPAFSCGKTTVDEPAMLQDVLGCR"
     gene            complement(19295..>20263)
                     /locus_tag="VFV99_03055"
     CDS             complement(19295..>20263)
                     /locus_tag="VFV99_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5058307.1"
                     /translation="LLRVVDPTVDGVSTMIRELAAGGVNVLILGETGAGKEVLAATIH
                     TLSKRRGSFVRVNCAAITEPLLESELFGHEKGAFTGADRRKLGLLEAADGGMVFLDEV
                     GELEAAAQAKLLRVIEDRQLRRVGGLDAIPIDVVFIAATNRDLTAEIAAGRFRADLYY
                     RLDGFSLTIPPLRERREEIIPIARMFIAMHARRMELPTPPALADDVIPILQAYRWSGN
                     IRELRNMMERAVLLAGGGAIQLEHLPLDNLKSSDELEESLARASADSEAPADLSAEER
                     TERDRIIETLASVYGNQTRAAELLGISRRWLTTKIARYRIPRPRKR"
BASE COUNT         3329 a         6665 c         6872 g         3397 t
ORIGIN      
        1 caacgtgaag tgagcgcaga ccgcgcgttc gacgacaact cggccgtctg gttgctcggc
       61 gcgcccgccg acacgcccgc accgccgctg gctcgcgatc tcgatgtcga tgtcgcgatc
      121 atcggtggcg gcttcaccgg cgtggcgacg gcctatcggc tgagccagcg ctttccgaag
      181 ctgggtatcg cgctactcga ggcgaagcag ctcgcgaacg gtgcgagcgg gcgcaacggc
      241 gggctcgtgc tcaacggcat caccgtgaaa gacgtcgacg cggatctgat ggtgcgcgag
      301 cacgcgatca cgaagcgcgc gatcgacgac atcgaggcgc tgatccgcga gcattcgctg
      361 ccggtccgct ttcgccgcac cggatgcgtg catgtggcaa cgaccgcgga agccgccgag
      421 catgcgcacg cgatgaccga gcagctcgtc gcgcgcggtt tgccgctcag gttcctccgc
      481 ggcgcagagc tcgatgccgt gttgccagtc cgcggtgcgc acggtgccgt cttcgatccg
      541 acagaagcga tgctcgtcgg cgtcgatctg atccgcgcga tgcggccgct gctcgtcgcg
      601 caaggcgttc agatctacga gggcacgcga gtcacgcgcg tgcgcgaggg cgcgacgatc
      661 gagctcgcca cgccgggcgg tgtcgtgcgc gcgaagtccg tcgtaatcgc gacgagcggc
      721 tacacgccgc gcctcggcta cttcacgacg ggccttctgc ccgtgatctc gcacgtcatc
      781 gcgaccgatc cgttgcccgc cgacctgctc gcgtctgtcg ggctcgcaaa gattgccgga
      841 ttcttcgacg actcgccacg cctcgcatac tgcagcgtcg acgacgccgg ccgcgtcgtg
      901 ttcggcggcg gcacgaccgc ggcgtacgcg tatcgcttcg gcaatgcgac ggcatacgtg
      961 cctcagcgcg acgatgcagc cgagcgggca ttgcgagcct cgctcgtcgg ctacttcccg
     1021 cagctcgccg acgtgccgat ccgtcatcgc tggagcgggc cgctcgatct cacactcgtc
     1081 cgtcactgcg cgatgggcgt gctcggcgag catcgcaaca tctattacgc cgtcggcttc
     1141 agcggccatg gcatcacgct cgcgaacctc gcaggtcgcg tgctcaccga tctctacgcc
     1201 ggcgagcacg accgctggaa ggactgcgcg ttctacatgc gcaagccgag cggcatcccg
     1261 ccggagccgt tccgctggat cggctatcac ctctacacga agctcacggg caagtcgccg
     1321 tggaagcggc ccgagtagcg ctactcgacg aagagctcga cgaactcgaa tttgaagttg
     1381 ccagtgcccg cggcgtcggc gagctccgag agatagacgc ggccattacc ggtgctcgct
     1441 tgtccgctgc cgggcgtcgc gttctgtggg ctcgcgggac gcacggtcca gctcgatgcg
     1501 gtgccggcgg gggccgcgcc gctgacgcgc tggaagatct gcgatgcggc gctcggcagc
     1561 tgggccatcg gaccttcgac gagcgtgcct tgtgcgttca gcagcgagaa cgtctcgctg
     1621 ccattgatct gcggaaactt cgcgcccgag ttgatgtaca cgacgttcga gccgagcgac
     1681 cggccgtaga acgcttcgag ctcggcacgc gttgcgtcgc gcgcgacgat cacatagccc
     1741 ccgtgcggaa cgacggtgcc agccggaagt tgaaagttgg cggccgcgtt ggcctggcga
     1801 atcgtgaacc ggctcacgtc gacgccctcg acggtccgcg cgacgccgtc ctctggtgcg
     1861 cggccgagcg tgccgaggaa ctcgagcacc ttctgctgat cgtcggacga tagcgctgcg
     1921 aatgcagtgc gcgccccgag cgcctcgccg gcatgcgcaa ggatcgctgc gcgtagcgtc
     1981 ggcgcgcgac cgtcgtgcat gtacggcggt gacgtcgcga cgccccacag cggcgtggtg
     2041 atgaactggg ccggcgcgat gctcttgaac ggcttgctgt cggcgagcgc gctgcccatg
     2101 tcgtggcgct tgaagtcgct ccacagctcg acggtcgtcg aaccgtcgct attgcgcgcc
     2161 gggtgcggcg acttcatgtc ggtcgccaga tcgaacgtga tgccggcgcc gccggtcgtg
     2221 tccgctggct cgacatgcgt cgtcgagctc aggcgcatct gcgcgacatg gcaatcgccg
     2281 caaccgacgc tcccaaacag ctgctcgcca gcggtggcac gcgcgcgacc attggtgtcc
     2341 gctggcggaa cccgcaccgg cgtctcgcgc agtcccatat aggttgccat ggcagtgagc
     2401 tgaccctcgg tgatctcgtc ggtcacgcca tcattgtcgg gatcgcggca gttcgagccg
     2461 gtgccgaacg tcgcggtgtc cggagccgcg cagtgcttcg cgatcgatgg cgcgctctgc
     2521 atgccgaagt ggacgcggaa gccaccctcg acaaaccggc gcagcgtcgc ctcgcgacct
     2581 ttccagccga acggcttgac gacgagatcc ggatcgacgc cttgcaggtt cgtgatgtcg
     2641 acgaagcctt gcggcgtcac cacgaccgag ccgaacgaga cccccttcga gctgagcgat
     2701 cgcgtcaccg aggttgcgct gctggccgcg gcggatttcg ctgccgcgag ctgcgcctgc
     2761 agctcgttcg tcatctcgat cccgatctgt tggcgcagac cgaggccgag gacgatcggc
     2821 ggattgcgca cgagccccga ggagcggttc acgccatcac cgttctgaaa gatgttgtga
     2881 ttgaggtcgc cggcaccatc gtcgcctccg acgttgtggc aggtgatgca gctctgcgct
     2941 tccggtgcgc cgaagccgcc ctggtggacg gtccgaaagt tcggtcgcgg gttggtaccc
     3001 gcgggcgcac cggtaagcgc gttgcctagc ccgtcttcga cgccgtagat gttctcgaac
     3061 aagaaatcgc cgacttcgaa tagctcgtcc ggcgtgaagg cgccgtgatc gatgtccgtc
     3121 tgcgcgatgc gcgagctctg gaacgtcatc tcgccagacg gcgcgcacgc gttgttggtg
     3181 cagatgagcg tcagacggta gtggcctccg ccctgttgag cgaagctggt ggcaacgatc
     3241 agatactcgc catcgacggg tagcgtgaag tcgacgatct cggagtcttt cgcgcacgcg
     3301 atgccgttga agaagtcgtc gttgcgaatc agatcggcgc cacgatcgcc atcgacgttc
     3361 tcgggcccga acaggtgcac cagcgtgtcg ggctgaccgc acctctcgcc actcatcgcg
     3421 atcgtgatct tgccgccggc cttgccgttg aacttgtagc cgtggaagtc gccttgctcg
     3481 agcaacgtcg tcgggttttc agggaacacc gccgtgtcga aggcgagcag accttcgtcg
     3541 ttaatcgggg ttgcggcggt gagcacgagc tcgtcgtcgt acttcgccgt ttcttcttcg
     3601 ccggaggtgg cgcagctcgg cagcgccgag aggccgagga ggatccacaa ggttcgtttc
     3661 gtgtagtgca tacagagttc cttcactgcg gtgagcaggt gttcggcgtg ggcgttggct
     3721 gatcgaccca cgtggtggaa gaggtgcgag agacgctgtg accggctgca acatcagggc
     3781 agcgcggcgt cgcggcggtg ccgctcgcgt cgagaaggcc ggcgccgttg ttcaggccgc
     3841 acagcacggc atcgagcgga atcgtcgacg gagacagctg cgtgatcggc acgtcgaaca
     3901 gcgcgaaacc ggcggcttgt ccgttcgtgc ccgagccgtt tattagatcg ggcgagaagt
     3961 tgtacgtctg gccgtagacc gggccgccgt tcgcggtggt ggaggtcggt ccgccgacga
     4021 cgaagcagcc gtgcgcggga atcatgcccg cgagctgcgc catcgagtac gtgtagccgg
     4081 tgcgccccga gccgaggctg tacgcggaca gatcgatcgc gacgtccgtc gtgttggcca
     4141 gctcgatcca ctcgttgccg tcatcggtgc tgatcggatc gaactcgatc tccgagagct
     4201 gcaagccggt gaccgcgatc gaggtcgaac gcgagatcga gcccgcggtc gccgtaatgc
     4261 gtacggcatg cgctggccgc ttgccgatca cgtcgatgtc gacgctcgag acaccggcat
     4321 cgacgtggta cgcgctcggc acgccgagca cggtcgcatt gtcgacgtcg aggtcgatcg
     4381 attggccgcc gggcggcgcg gggagcgcga gcgtgatcgt gatccgggtg tgctcgcctg
     4441 cgcggatggt cgtggcagcg gcggcgatag aggcgacggg ggcatcgacg gtcattcccg
     4501 cgtcgggcgg catgtcgttg tagtcaccca tccgctcgca gcgcgcgccg gcgcggatcc
     4561 atgcctcgat gcgtgcaatc ttctcggcgt cgagccgcgg tgcgccgaac ggcattggct
     4621 cgttcgaatc ggcgcagccg gtcgccggcg ctccgtcgag gtgcgtgccg gtgagcttgt
     4681 cgagcaagaa gctcagctcc ggcttgccgg gaacgacgag catcttgttc gggaacaagc
     4741 acgacgtgtg gccgacgagc gcgctgcaca tgtcgtcgct gcgcagatcg agatgtgctg
     4801 caggtgtggg accgccgtga cagctgccga agccacagct cgtggcgaag acgtcggagc
     4861 ggatggcttc gaacgttggc tcgtcggttt caacgatccc gccgggtggg cggacatcgg
     4921 ttccacatgc gctcgtggcg agcacgagga tgaacaaaga gactcgcatc agtgcacctc
     4981 acaaattccg ggcgtgccaa ccggctgggt cgtccagcgt tccgacgtcc ggacgtaggt
     5041 tccggatgca ggagccgcag cgacgacgtc ggcgagatcg cccgtcgggt cgagtaggct
     5101 gttgttgtcg ccgccgtaaa cgagcgcgtc gtacggcagc gtggtgtgcg tgatttgatc
     5161 ggccgagttg tcgaacagcg cgatgccgct cgcgtcgctg ccggcgacac cgagagccgg
     5221 tgagaatccg ttgacgacga cgcagccctt cgctggcact acaacgtcga gcggtttgcg
     5281 catcgcggtg tagtcgtcgc ggcccgcacc gaggctgtac gtggagagat cgatcgctgc
     5341 attcgagagg ttcgcgatct cgacccactg gttcgtcgtg tcgcccgccg tgcccgtgta
     5401 gacctcggag agcacgagcc caccgatcgt ggtgaccgca gtcttcgagg tgccccccag
     5461 ggttgcggtg acggtgacgc gatgaggacg gcgcgcgcgg gcagtgaacg tcgcctcgga
     5521 ctggccggcg gcgatctcga cggtatcggg cacctcgagc gccatcggat cgatggtcgc
     5581 gatgcgcacg cgcgcgccgt cgctgccggc gggctcgctg agcacgatgc ggtatgtggc
     5641 gagtgcatcg agtggcaccg gcgtcttcgg tccctcgagc gcccgaacct ccgcagcctc
     5701 gacgatgcag aacgtcgaga gctcttctgc ggggtagtag tacgcgacca tgttgcagtg
     5761 ctcggaccgc ttggtgtccg caccccacgt gatcggcatg tcggtgtgat tgaagaaatc
     5821 gcactcgaac tgcaggccat cgccgtcggg gacgggcggc ggcggatcga agaccttgaa
     5881 ctggaacgtc tgctcgtcga gcgcgtcgta aaccggtgac ccgtcggctg cgccctcggg
     5941 ccgcgtgtcg atcgagtagc gcgtgccgag gccgtggaag tgacccatca ggccgagcag
     6001 ctcggtgtga gagggcagcg gacaccactg gcggatcacc ttgcgttcgt gcggctgcat
     6061 cgcggtttgc ttgttgatgc cgaacacgac gccgacgtgc gcgtcggaat ggtcggtggt
     6121 gtggaacgcg agctcgacgc tgccgtcgat cggcgtgttc gtcgtgttga gccagtgcac
     6181 ctgcacgagc agctgctgat gcggggcgag cggcacggtg cgacccgccg gcaggctcca
     6241 atcgatcggc gctgtttgcg cgccgagcac caggtgccag cgcgtccagt cgatgccctg
     6301 ccagcaatcc gagacatcgt cgtcgacggg cgtgtcggac ttgtaaatgt gaacgtggtg
     6361 gctgccggcc gcgaactgaa cggtgatgcg ctcgacttcg atcgggtcgg cgttgtcgag
     6421 cttgaagacg tgacagtcct ggatctccga gccgggctcc tgatgccagc gcaacgtacg
     6481 ggtgaacgtc tcgccggagt cggcatgaca gccgagcgtg gacaccgcga cggcggcgac
     6541 aaagcagcga gcaagaaacg agagcgacat tgcggccgct gaaatagcga acacaccggc
     6601 gatctgtcag ctcgcgccag atctgcgcgc gcaacgcgca accatcactc gtgatccggc
     6661 gttcacgccg agctggtgac caccaggtgc tcacatcgcg cgctgcgttg acgcggccaa
     6721 gtacgcgaaa gtctcaccga tccctcgagt agcttttttg tgcgaccggc gccctcgcgc
     6781 cggctccact ctgcgtgccg caaccgctca agactgcgtt cgatagcgct cccatgcgca
     6841 tgcccaaagg aacacccgca ccccgccggc aaccccggag tcgccatgcc gtgatcacgg
     6901 tcgatgcgat cctcggctcg gtcgagcgca tcctcgaaca ggacggaccg tccgcgctca
     6961 cgacgaaccg catcgccgaa gtcgccggcg tcagcatcgg cagcctctac cagtatttcc
     7021 cgaacaagga ggcgctcgtc ggtgcgctgc aagacaagta tgccgacgac acgctgagcc
     7081 gtgtgcgctc cgtgctcgtc gacgccgagc ggatgccgat caatctcgtg atcgcgcgcg
     7141 ttgccgaagc tgtgctttcc gcgcaacacg accagcgtcc gatccatcgt tggctgaacg
     7201 agtggcgctc cgtcactgga agtcacgagc gctatcgccg cgcactcgac gagcacgtcg
     7261 agctgatcgc cgattttctc tcgcgccggc ccgacgtgca tctcagcgat caccacgcgg
     7321 ctgcgttcgt gctcgtccac gcgatcgagg gcatcatcga agccgccagc gaacgcgggg
     7381 ccgaagtcga cgcgaccgcg atcgccatgc aaacgatccg catggtgacc gtcttcatcg
     7441 cgtgagctac agcagccgct cgcggtagcc gtcgggcgcg actgcgcgat acagccgctc
     7501 gatcatcgcg agcgcgtcac ttgcgggtac cgtgatcgcg gtcttgccgg agggcgtgcg
     7561 aatgccgagc tggaagtcgc gacccgcggc gtcgtcatcg atgaacaggc cgccgtaggt
     7621 ctcgaggtaa ccgagccgca gcccgtgcag cgcgacgcgg aacaacatgc gtgcgagcca
     7681 gtagcgcggc accgtggtga cggtatcgcg cacacggatc gtgacctcgg tcggcgacgc
     7741 gtgctcgacg gcaagctcgc cgtgatgcag cggcaagttg ccgcgcgcgg cgtcctcgaa
     7801 cggcgcgaac ggcgagggtg gcaacccgcg ccacgacggc ggtgcctcgt cgggagacgc
     7861 atccgtatcg ctgagcacga acgcgcgcac gacgccggtc gtgtcgagcg acgcgccggc
     7921 gggaatgcgg atcagatcgc aggcggcgtg cgcactcgtc ggcgctgcct cgacaatcac
     7981 gacccaaccg ccacgcgcag gcgcgatcgc cgtgggccga tcgacgagct cgaacgacgc
     8041 cgtgccgatc ttcgcgacaa ccccgggctc gccgctcacg gccgcgatca tcatcggcgg
     8101 tgcaggtgtc gcgtacggct tgatgtcgcc gtcgcggccg ctcggagcac cacggcgtgg
     8161 tgcgctcgac ggcgtgtgct ggttcgcgca gaacacgagc gagatgccgc agcgccgaac
     8221 gccgggcggc agctcgaacg gttcgtaagg cgggcgcaga cgacccggcc agtggagata
     8281 gccgatcgga tgcagcacga gatcgctgtt gcgatgattg tagcggccga cgtcggcgcc
     8341 gctcgcggca tcggagagga acgtgcgcag cggaaagcga tcgacgaggt cgtcaccgca
     8401 cggtagtggg atcgcgtact cgtcggagag attgacgtga ccgaagtaga gcggttccga
     8461 cgcggtcacg atctcgagct gaacccggcc gatggtcgcg cacacgatca ggtgatgctc
     8521 gaagcgctcg ggacgcgcga cgatctcgcg cagattgatc cgcgacagcg gcgcgggtag
     8581 agcagggcgg ctcgccttgg gaaacaccca cgtgttcgtc gcggcgtgcc gcgcgatctc
     8641 cgagcgcggt gtctcggcga gcttccagcg ctgcgccgct tgcgcgacgc gacggccgag
     8701 gtgctcggcg gtgcgtacgt cactctcgtc gagcgtgcgg ccgggcggtg tttgcgccat
     8761 cgcgccgagg tgactgccga gccgattcgt atcggtcgcg accggttctg gcgcggcgta
     8821 cgtcggtggc aggccgaggc ccacccagac catgccgtgc tgcgcggcga ataccgacag
     8881 ctgcatcagc gtcgcgagct tgtcgccgct cggcgcagcc gagtgcgtga acgcggcggc
     8941 gagcttgtcg cgccaggctt gcagcgccca gatcgcgctc gtgccgtcca tgaacgactt
     9001 gaacgcgccc gacgcgctgc ccatgtacgt cggacagccg aacacgatcg cgtcggcggc
     9061 ttgcagcgcc gcgtggtcga cgttggcgac cgagaacacc tgcgtctgcg tgtccgcgac
     9121 ggagccagcg ccacgcgcga cggcatccgc gagcgcacgc gtgcggccgc tcaccgagtg
     9181 atagacgatg gcgacggtgg tcacgtgcgc tcctggatcc acggcacgac aatgtccatc
     9241 agcgcttcga cgtgtggcgt cgccttctca ccgagctcgg gctcgaagta gtggcgcgct
     9301 tgctcgacgg tggtgacgcg cttgtcgcgc gcaacgatcg ccttgtcgat cgcggcgata
     9361 tcggccggaa agatctcgcg atcgcgtccc gcgtgcacga gcagcgtcgg tgcggtgatg
     9421 ttcgcgacgt tcgcgacgag gtccgccttc gacgacaacc ccgaccacgt cgacagccac
     9481 gcgcgcggcg tgcacgtgcg cgcgagcccg agcgacgagt agttcatgag atcggggcgc
     9541 tcgctgagca gcgacccgta atcgcggccc gacggatcga tacgcttgtc gacgaatgca
     9601 gggttcgcgt tcgtgcgatg gacaaccatc atcggatcgg cgacacggcg gcgcatgatg
     9661 tcgcgacgct cgtcggcgga ccgcgatgcg aagtcgggtg ccgtcgccgc ggcgctggca
     9721 tcagccgcgc gctgcagcag ctcgcgcgcc ttggcgtcga gacgggcaac gcgggcgcgc
     9781 tgcgcggcgc gatacgtcgc gaggaacgcg tcgctatatt ccgaccacgc cggcggctcg
     9841 gcaaaaccgt ttcgcgcatc gtacatgtcg agcgctggat cgaccgacag cggatcgttc
     9901 tcgtcggtga ccgacggatc gatcgcctcc atcagcacct tgccttggcc gcgatgcggt
     9961 gcggtgtaga tgatcgcgtg tgcgggcgtg agcggcgcgc tctcgaagtg cgtcgggtta
    10021 ccggccggcg atcgcgtgat gcgctccgcc gctggcaacg tcgcttgcgc ctggtagtac
    10081 gcggagagcg acgcgccgcc gcaattgccg agcagcacga ccttgtcgac gccgcgcttc
    10141 tcgagcaggt gacggacaca cgccgccatg tcgagcacca tctcttcgtg ctcggccatc
    10201 gtgtcgttgc cggcgtggcg cgtgttcgcc gcgagcacac cgaagccggc cgcgacgagg
    10261 cgcgggatcg aatagtgatg cgagaagtcg acgcgcgggt gcatgacgac gacgccgacc
    10321 ttcggcgctg cgtggcgctt gctcgtccag tagtgcgcgc gaaactggcg caagtcgtgt
    10381 gcgcgcagcg cgagcgattc gacaacggcg tcttccggtg gcttgcgaaa ccagaacgga
    10441 atccccgccc agtacgtttt cgacaccagc atcatgtgga gcgaagctac cggtggaaga
    10501 ccatcgtgtc gagggtgccg tccgccccag ccatcacgag gacgtgcccg gtcatgaaga
    10561 ccgccgagag gtcggccgtg gtgcggctga tcatcggctc gatggcggtt ccgttccagt
    10621 agcgcagtgc accctgctgg ccgacgccaa acagccgatt gctgggcgtc aggcatgctg
    10681 cgatgagccg gtcatctttg aacggacccg cgctccatgt cgcgccgtcg tagtgcagcg
    10741 ggctcttgcc gacggcatag atgtcgttcg ccgacgtgcc gatcagcgtc gttgcatccg
    10801 tcatcgtcgt tgtcgtcgtc cacgacgtgc cgtcgaagtg cgcgaaggca gcgccagcga
    10861 cccacacatc ggtcgcgctc gcgccccaga tggcacgtcc ggtggtgatt gtgacccgac
    10921 tgaatgtcgt gccgtcgtac tcatagatgc cgctggtcga cgcaccgagc actcgattcg
    10981 ttccagtagc gagcagcgac gtaaaattcc cgccgccacc accgaccgag gtccactgcg
    11041 agccgttgta atggagcagc gccagagaat cgcccgccgc gtagatgtcg ttcgccgccg
    11101 tgccttggat cgcgtgcagg tcgaagctgg ttggcgaggg ctgctgggtc cagctcaggc
    11161 cgtcatggtg caggatcgtg ccgttgcggc cgaccgcata gatgttgtcg ggcgcggtgc
    11221 cccagatgct gtacagggtc tcggacgtgg gctggatcgt cggagaccat gccgcgcctt
    11281 cgtagcccat gacgtcgtcg gagccgacga tgccgaccac gacgccttcg atcccggtga
    11341 ccgaaaacag cgtctgcgcc ggcgagccgg tcacgccgcg accgagcgcg agtggccgcc
    11401 agaccgcgcc gtcgtaaaac agcaccgtgc cgcgctcacc gaccgcgaag aaaccatcgg
    11461 tcgtgctgcc ccacacgctg cggaggttca cggtcgtcgc ggttcgccct tgcgaccacg
    11521 tgccgttgct cgcacgcatc ctgatcgtgg ccgcgtcgcc gaccgcgacg accatgccgc
    11581 ccgccgccca cacgctcact agatcgacgg tcgtgcccgg atcttcggtc gtccattgcg
    11641 tgccgtcata gatcgcgacc ttgccttgat caccgacgac gtacacgtgt gtcgcatctc
    11701 cagacaccgc gcgatagttg ccggcgggcg ggctcagcgc cgaccagatc gtgccgttgt
    11761 agtgcagcac cgtcgaatcg ccgaccgcat acacatcgct cgccgagcgt ccccacacgt
    11821 cgcgcagatc gacggtcgtc ccggtcgtgc ggacgaccca gctcgttccc gagtaatgca
    11881 ctgccttgcc catgccgccg acggcgatcg cggtggtcat gttggccgcc cagatcccac
    11941 cgaacggagt gctcgagatg tacggattgc cgaccatcat gtagttcgtg tacgtgttcg
    12001 cgcggttctc ggcgatgtag aagccgttgc tctcggtcac gcccgcaagc ttggtgacca
    12061 gcggagtact ggtgctcgac atcaccggct gcttcacccg cgtgcgaaag ccgatgtagc
    12121 gacacgtctc gggcttgatc acgggctgac atgacgccga acatgtcgtc aatccgcggt
    12181 cgaagacggt gccggcgcag ctgaccgtcg cggttattcc gggatcgcag ccttcgtcgg
    12241 cggacacgac tccgtcgccg cagcgcaggt ggcagtccat gctgcacggg ccactcgtat
    12301 cgctgttcgg tccggggcac gccgggttgt gctcggtagg atcatcggga tcgaagccgc
    12361 agtcgcactc ttcgtctgtg tcggtgatgc tgttgccaca cacctcggcg gatcggcagt
    12421 cggcgctgca gccatcgccc gagacgagat tgccgtcgtc gcacgcctcg ccgtcgtcgg
    12481 tgatgccgtt gccgcagccg gagctgatgc atgcgccgcc gatgcacgtg ccggcgaaga
    12541 tgtcggtcgt cacgcagcgc gcgccgctcg cgaggccgtt gcaggcggtg aattgctcgt
    12601 cggtgacaca caccgtcgcg ccgcccgccg tggcgcagaa cagcccaggc ccgcagacgc
    12661 tgccgtcggc gcaatgctgg gtgcgcgggt ccaaacagcc ggccgcaacg accagcagga
    12721 ggatgcaccc cgcacgcatt gcgcacagta tatgatgaac gatcggtggg tgagaacgac
    12781 gcgacgcgac ctccgagcgg gcaggcccgc ggggccgccg gcatgcgtgc gcgcgtcggg
    12841 caatacgagc tgatccgcga gatcgggcgc ggcggtatgg ggaccgtgta cctcgcccgc
    12901 gatacccggc ttgcgcgtcg cgtcgcgatc aagttcatcg cgtacagctc ggccgcgatg
    12961 aacgagcgct tcctggtcga agcgcgcgcg accgcgcaat gcaaccacga gaacatcgtc
    13021 gtcatccacg aagtcgacga gctcgagggc cagccgtaca tggtgctcga gtatctcgaa
    13081 ggcaagtcgc tacgcgagct gctcgacggt acgccgatgt cgccgggccg ggcgatcgag
    13141 ctaatcgtgc cggtcgcgcg cgcacttgcc tgcgcgcacg agcggtcgat cgtccaccgc
    13201 gatctcaagc cggagaacat cttcgtcacg aacgcgggac aggtgaaagt gctcgacttc
    13261 ggcatcgcaa aggtgcacgg cgagcccgac cgcgacggcg acgacgaagg caccggcgaa
    13321 cacacacagg cgagcgggct cgtcggcacg ctcgcctaca tggcacccga gcaatggggc
    13381 gccgacgacg tcgatcaccg ctgcgatctg tgggcgctcg gcatcgtgct gtacgagctg
    13441 ctcgccggtc gccatccgat ccagccgaca acgaaggatc gcttgatgag cgccgcgatg
    13501 cgccccgacg agcccatgcc ggcgctggga tcgccgtcga tcgacgcgga gctgcaacgc
    13561 gtggtcgcgc ggtgcctcgc caagcgcaag gccggacggt tcgcgacagc tgccgagctg
    13621 gtcgccgcgc tcgagccgct gttgccacgc cgacgcgctg tcggtgcggc cgagatcgag
    13681 ccgtatcccg gcctgaccgc gtttcgcgaa gaagatgccg acctatttca cggccggcgc
    13741 tccgaggtcg ctcgcctcag cgcactactc cgcgaccggc cacttgtcgg tgttgtcggc
    13801 gcgtcgggcg ccggcaagtc gtcgctcgtg cgcgccggat tgatccccga gctcaagagc
    13861 cgcgaagcct gggaggcgct gatcacgcgg ccggggcgtc atccgctcga cgcgctcgcg
    13921 gtcaccgtcg ccggcgaagc agacagcgcg agtcttgcga ccaagctcgc gaacgagccg
    13981 ggtgcactcg gcgtgcgatt gcgcgagcgg gcgcgcacgc gcggcggcaa gattctactt
    14041 ttcgtcgatc agttcgagga gctgtatacg ctcgccgacg atcgcgcgcg tgccgcgtac
    14101 ctcgcgtgct tgcgtggcgc ggccgacgat ccgctgtcgc cgacgcgcgt tgtcgtgtca
    14161 ctgcgctcgg acttcctcga tcgcgtcgcc accgatgcgc aattcgtcga ggcgttgtcg
    14221 ccgggcatgc agttcgtagt cggcgccgat cgcgacggtt tgcgcgcggc gctcgtcgag
    14281 ccggcgcagt atgcggggta tcgcttcgag accaccgaca tgatcgagac gatggtcgac
    14341 gagctcgcga ccaccgcggg tgcgttgccg ctgctccagt tcgccgcgtc gtcgctgtgg
    14401 cagcaacgcg accgcggcca gcgattgctc acgcgtgctg cgtacgatcg gatcggtggc
    14461 atcgccggcg ccctcgcgag ccatgccgac gcagtgatcg cgagcatgcc gcccgccacg
    14521 cgtgacatcg cacgtcaagt gctcgttcgc ttggtcacac ccgagcgcac gcgcgacgtc
    14581 gtcgaggtcg cagagctgca cgagctccgc gatgcacgcg cggtgatcga tcatctcgcg
    14641 gcggctcgcc tcgtcgcgat tcgctcggac caaggtccgg ctgtagtcga gctcgtgcac
    14701 gagagcttga tcgagcgctg gcctgcgttg cgcggctggc tcgatcagac gcaaggcgac
    14761 accgcgtttc tcgcgcgcgt gcgcacggcg gcgaagcaat gggacgatgc agcacgctcg
    14821 ccgggtttgc tctggcacgg ggatcccgag caggacgcgc ggcgctggct ccgcgactac
    14881 gccggcgagc tcggcagccg agagcgcgcg tacctcgatg ccgtattcca gcaagccgat
    14941 cgcgcagcac gcaggcgacg attcgcgatc ggcggcacga tcgcgatgct cggcttgctc
    15001 gtcgtcgcgg ccatcgttgc gctggtgttc atccgcgatg cgcagaccga ggccgagcgc
    15061 gaggcatcac gcgcgcacga tgaagcgaac aaggcgagca aggcgaaccg cgatctctcg
    15121 gatgcgctcg cgaacttgca gcgcgagacc gctgcgcgcg aagcagcgcg ccaggcggaa
    15181 gcggctgcca ctcaacaggt caacgcggca cgtgtcgatc tcgagcgcgt caacggcgac
    15241 ctgcgtatcg cgctcacgcg cgccgaagca gaatcgaatc gcgcgaagag cgaatccgaa
    15301 cgcgcgaagc gtgagtcgga gcacgcacag accgaatcgg accgcgcgaa gtcagaggcc
    15361 gagcgtgcag agcttgcgcg gcgcacggcc gaggaggagc gcgcccgcgc cgaggacctc
    15421 gctcgcaagg agcgcgagcg cgccgaacgg cgcggcaagc tgtcgaccac acttccatag
    15481 gtcgcgaatt gggaattccc taattgggaa gcgaagcggt tgggaacggt tgggaccacc
    15541 gtggttacag cgagttagga gtggcatcgg cgctgcacta agggctcgcc atgatgacca
    15601 agctcctcgc tcttgttgcc gctgtctcgc tcgccgcctg tgtcgacgac acctccgcac
    15661 ctaccgacga gtccgcgggc agcatcgacg tgcagaagga cgagcaggtc gtcgagcccg
    15721 tggggccaac ggacttgtgc gcggtgttgc cgaccgatga agcggacccg tgcagccatt
    15781 tgtgcgacga ggaaggcgga ggcatcaacg cgttcattcc cgagcacacc tgcgtgctgt
    15841 tcgtgtgtca cctcaccgac ggctcgacgt atcacgcggg cggttgcgtt ccctgagctg
    15901 atcggtcgcg tgtatgctcg aaacaatgac tcgcggctgg gggcttgtga gggcggtgct
    15961 cgtgctctgc acgctctcgc tcgcgcatcc agcatcggcc gatacgcggc cgtgggcagt
    16021 cggagtaagc gacgccgatc aagcgcgggc gaaggagctg ttcgagcagg gcaacaagtt
    16081 gctcgaccag agcgcgttcg gcgacgcgct gaagctctac gaggaagcga tcacgatctg
    16141 ggatcacccg gcgattcgct acaacatcgc cgtcgcgcag atgaatctgc agcgaccgat
    16201 cgaagcgtat cgcgagctcg aacgcgcgct gcgcttcggc gcggccgcac tcgaagcgga
    16261 cgtctaccag caggcggtca cgtatcagcg aatcatcggc gggcagatcg tgcacctcac
    16321 gatcgattgc gtcgatgccg agacgcgcat cacgctcgac aacaacgagc aacgcattcc
    16381 gtgtcccggc tcgtccaccg tgttgatcac gcccggcggc cacaagctcg tcgcgagcaa
    16441 ggcgggctcg ctgacgaaga cgatcaatat cgcgcccagg ccaggtgatg cgccacgcga
    16501 gctgatcgac ctgatgacgc tcaccgaagc gacggtgacg cgccatcggt ggccgcgctg
    16561 gaagccgtgg gtggtcatcg cggcgggcgt cgcggtcgcg ggcgttggtc ttggcttcga
    16621 gttgcaagcc gcggcgacgt atcgcagcta cgacaccgcc gtcgacacgc tgtgcggcac
    16681 gatgccatgc tcgaacttgc cgccggttgt cgccgacgca tacgacgacg gccggcgcca
    16741 gaaccggatc gccgtcgggc tattcgcggc tggtggcacg acgttcgcgg ccggcgcggt
    16801 tctgctctgg ctcaaccgcg cccgcagcga gcgcatcggt tacgaccgcg cgtcgatcgt
    16861 caccgcgaag atcggtgcac acgcgtcgct ggtcgcggcg caatggacct tctagtgcgc
    16921 gaatcgagaa ttctcgaatt gagaacgaac gcggtcgcga gacgcgcgcg cgtcgcgatt
    16981 gcgcgtggtt gcccgagcga gcgaatggca tcgcgttcgc aatggtccgc gaacatgtcg
    17041 cgagcgatgc ttgctacgct cttcctgttc gctggttgcg agaaggatct cctcgagaac
    17101 gttcacgacg cgggcgccga tgcggacctg ccggtcgtcg atgcgccggt cgggcgctgc
    17161 aaggcgccga cgaccgatcc gccgccggcg gcgcagtaca cgctgttcgt gaacttcgac
    17221 ggtgtgacgc tcggcgtctg ctcgagcccc gactcgcggg cgaattgctc gaacctcatc
    17281 cagacagaca ccacgttccc tgcgttcctc gacgggaatc cggcgcgcga cagctttatc
    17341 gcgtcgatcc tgttccccgt gcgcaaggcg ctcgcgccgt actcgatcga cgtcgtcacg
    17401 acgcgtccgg cgtccggcaa ctactacatg atggtggtcg gcggcacgcc tgcgctcatc
    17461 ggggcaccgt caggtgtcgg cggcatcgcg ccggcgagtt gcaatccggc cgatctgcgc
    17521 aacggcgtca gcctgatctt cgatcggggc caggcgccga cgacaagcga ttatgcgagc
    17581 tcgatcctga gcgacatggg cgtgttcgcc gcgatcccgg tcaccaactc gccggtgacc
    17641 gattgcctgt gccgcgtcaa cggctgcgac ttcaaaggcg ggctctgcac gtacggcgtc
    17701 aactcgaccg tgaaccccga tccccagttc aactgtggcc tcaccgccgt cgacgagccc
    17761 gcgatgttgc aagcggtgct cggctgccgc tgagggcgaa gctcatgcgc aagtcacttg
    17821 tcttcgtcgt cttgctcgcc gccgggtgcg aacaggatct gcttgccgac gtgcacgacg
    17881 ccggtcctga tggtccgccg gacgccgcgc attgcaccgc gccgctcacc gacgcccatg
    17941 cgcccgcgat gcacacgctc tacatcaact tcgacggcgt gacgctgacc cactgcaaca
    18001 aagccaatgc gccgggaaac tgctctgaca tcgtgcaagg cgacacgacg tttcctccgt
    18061 tcatggacgg attaccagca cgtgatggct acatcacgtg gatcgtggat gcggtgaagc
    18121 agagcctcgc accgtactcg atcgacattg tcacgacgcg gccggcgccc ggcaccagct
    18181 actacatgac cgtgttcggc ggcacgcccg cgttggttgg cgctgacgac accgtcggcg
    18241 gtatctctcc cttcacctgc agcgccgcca accggaatct ggtctccatg atcttcgact
    18301 tcggtgagaa caggacgacc tacgactacg cgagctccgt cctcagcgac ctcgcgatga
    18361 tggcggggct gccggggacc accggaccga acaacgactg cgcgtgtcgc tcgtgcgcat
    18421 acgaccagta tcagctctgc acgtacggtg tcgactcgcc aaccattccc gacgagcaca
    18481 gctgcggcct cacgactgca gacgagcccg cgctgctccg agcatccctg ggctgccgct
    18541 agatggagtt tatgcggtca acgctgttcg ttctgctcat catcggcacg ggttgcgaga
    18601 aggacttgct cgccgacatg cacgacgcga ccgtcgcgga cgcgcagcag gtcgaggtcg
    18661 atggcgggcg ttgcatgtca ccgatgaccg atgcgcaccc agctgcgatg cacacgctgt
    18721 tcgtcaactt cgacggtgtg ctgctgactc cgtgcggcac ggccgacgcg cgcctcaact
    18781 gcacgggatt ggtgagcacg gagacgatga tcccgccgtt catggacggt aaccctgcgc
    18841 gcgacgcgtt catcgcaacg attctctatc ctgcgcgcaa ggcgctggcg cggtactcgc
    18901 tcgacatcgt cacgacccgg ccgacgagcg gcgactacta catgatgatc tacggcggca
    18961 cgccgcagtt actcgggcta ccggcggcga acgtcggcat cgcgccgttc agctgcatgc
    19021 ccgacaatca caacaacatc agcttcacgt tcgatcgcgg acaggcaccg accaacatcg
    19081 attacgcgga cggcattctg agcgacatgg gtgtgtttgc cggaattccg ggcaccacgg
    19141 tcccgggcga ctgcttgtgc cgcagctgcc agtttccgac cgacacggtc tgcacgtacg
    19201 gcgtcgacgt tccagtgtcc acagatcctg ccttcagctg tggcaagacg accgtcgacg
    19261 aaccggcgat gctgcaggac gttctcggct gtcgctaacg cttgcgcggg cgcgggatcc
    19321 gatagcgcgc gatcttcgtc gtcagccagc gccggctgat gccgagcaat tcggcggcgc
    19381 gggtctggtt gccgtacacc gaggcgagtg tctcgatgat gcggtcgcgc tccgtgcgct
    19441 cctcggcgct gaggtcggcc ggcgcctcgc tatcggcgct cgcacgcgcg agtgactcct
    19501 cgagctcgtc actggatttc aagttgtcga gtggcaggtg ctcgagctgg atcgcgccgc
    19561 cgccggcgag cagcaccgca cgctccatca tgttgcgcag ctcgcggatg ttgccgctcc
    19621 agcgataggc ctggagaatc ggaatgacgt cgtcggcgag ggcaggtggc gtcggcagct
    19681 ccatgcggcg tgcgtgcatc gcgatgaaca tgcgcgcgat cggaatgatt tcttcgcggc
    19741 gttcgcggag cggtgggatc gtgagcgaaa agccgtcgag gcggtaatag agatccgcgc
    19801 ggaagcggcc ggcggcgatc tcggcggtga ggtcgcggtt cgtcgccgcg atgaacacga
    19861 catcgatcgg aatcgcatcg agcccgccga cgcgacgcag ctggcgatcc tcgatcacgc
    19921 ggagcagctt cgcttgtgca gccgcctcga gctcgccaac ttcgtcgagg aacaccatgc
    19981 cgccgtcggc ggcttcgagc aggccgagct tgcggcgatc agcgccggtg aacgcgccct
    20041 tctcgtggcc gaacagctcg ctctcgagca agggctcggt gatcgcggcg cagttcacac
    20101 gcacgaagct gccgcggcgc ttcgacagcg tatggatcgt cgcggcgagg acctccttgc
    20161 cggcgccggt ctcgccgagg atcaggacgt tcacgccgcc ggccgcgagc tcgcggatca
    20221 tcgtcgacac gccgtcgacc gtcggatcga cgacgcgcag gag
//
LOCUS       DASUFX010000079        11827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_290211,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000079 DASUFX010000000
VERSION     DASUFX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290211"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFV99_03060"
     CDS             <1..193
                     /locus_tag="VFV99_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058308.1"
                     /translation="VDKAATNYRWGGLQCKGRDFSETSIEILFAAFRSEYQVKLEFVI
                     SDYKDKQYRCVTGFTVSKS"
     gene            190..1614
                     /locus_tag="VFV99_03065"
     CDS             190..1614
                     /locus_tag="VFV99_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5058309.1"
                     /translation="MMRVLLLLVLCVPARAFALGEVSPILFLNRCTNNCTVNGGVDDA
                     RAMSSSIPCAAGASCGGGSCSCNGTSAGTYTVEEFKNYMGQTGAAADEEWNMIVQCVR
                     EVYSPYNIAVTDVFPTGMAHTQGIVAGLPVNIGYGGLNTGGLGGTSSCEPVDNVISFT
                     FANIFTSTSQQARVRTLCYVVAQETGHSFGLDHAFEFSDGRSACTDPMSYRNDCGGQR
                     FFRNDLARCGENGPRPCKCPNLQNSHQKLLAIFGPGMPLTGPPTLTVSSPTDGATIQN
                     GTPVIATAFAQRGVHHIDLLLNGYKWLTVKGAPFGGNGQPETTYPLVLPSGVPDGIID
                     IVVKAYDDIDAETDATLTVTKGAPCTTADTCAKGQKCDAGKCFWEPPVGELGDPCTYD
                     QFCKTGSCVETTSGQFCSNVCVVGVDDSCGTGFTCEGAAGGNGYCVPVGAGDDTCCGI
                     GANGKTSALLSLFVVGFVLRRKRR"
     gene            complement(1615..2343)
                     /locus_tag="VFV99_03070"
     CDS             complement(1615..2343)
                     /locus_tag="VFV99_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058310.1"
                     /translation="MRTPLAILLMSIALLACSKGANEGAEEAKKQAEKEQKEKEASGG
                     VAKKISPPVAGKAKLDCASVINATQFQQVLGETEAITVTDSKSEPDAAASCSILRGGK
                     RLSDAEQKAKLKKEGRLGVMAGDEICNVSTFCWTIEDADRFRQKCHDMKDKDDDTLGF
                     YSCVLIVAQGADDVKRFRFFDDDTKCIIQVRGGPSNVDNDLIGKCAKAAHDLIGPDQI
                     KVGAPPAEAPAAPAEPAAEGSATK"
     gene            2396..2707
                     /locus_tag="VFV99_03075"
     CDS             2396..2707
                     /locus_tag="VFV99_03075"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HEX5058311.1"
                     /translation="MRYHHTKNKGDFGLVQAMADLTAKGWAILVPLTEHEAFDLVACR
                     EGRFLRVQVKYRAMSRGCVTLHFRSIKPEQHPEGRYLGERLCPDPTDSPRSSGGFRPA
                     T"
     gene            2761..2834
                     /locus_tag="VFV99_03080"
     tRNA            2761..2834
                     /locus_tag="VFV99_03080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2795..2797,aa:Arg,seq:tct)
     gene            2894..3805
                     /locus_tag="VFV99_03085"
     CDS             2894..3805
                     /locus_tag="VFV99_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5058312.1"
                     /translation="MKARAFVRQLGKEVVNDGVTDLGAMMAYYSLLALFPMLVFVISL
                     SMLVLDPETIRQGADMALNAAPPAAREVIEPRVNGFIEASHTGFAIAGALLALWGASR
                     GAVAMMTALNTIHQKKETRSWIKRQAIAIGVTVAVAVFIVIALGLLFVGPFVGHYVAD
                     YVGLGDTFDLVWGIGRWVGAGLLVMFVWAIVYKFLADTNAPFRVFTPGAICGVLVWLG
                     LSAGFSVYLQHFNSYEKTYGTLGGVIIFLTWLWLSNVTMLFGAEINDVLADFRAHKST
                     AAAQLSHATRRKDDGSARHGTGTPATT"
     gene            3983..6349
                     /locus_tag="VFV99_03090"
     CDS             3983..6349
                     /locus_tag="VFV99_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058313.1"
                     /translation="MKKLMACALVATMSGCAPDIDVDHDEVAGPTVEFDPARSQATGA
                     RFIPFPNDLVRDPTTGKVNLPPQACETPAQKATRENILNKLDGFGTYETAMQVTFTND
                     VDEASLAGHIVMFQLTDEGVPIMPSAATQPIPVTVVRTGLSPRFEKGNDFCTPMDANN
                     VPSLTMVPSVPLKQKSTYFVALLKGITDTSGTEFGASPTWGLVASQTPPVTLDAAGNV
                     VADRTPLDPSIPEQRAQLVALAGLWQLHQPGIEFVQATTGAARSDILVGFQFTTQTTT
                     DQLDPSVTGSPANKLSTVGVLMTESVTGKFGPYSALCGAEATTAPAQCFLKLALGGCS
                     PLTTGCDANNYNAGNVACQFLYNCAAVGDVIGGGILTANYQTQIDNPFDAKHPIQGAW
                     SDPINPEQQATLTLETIIVLPAGTPPATGWPTILFSHGLGSSKESVFAIAGRFAGAGF
                     ATVAIDTAAHGSRAVRISDDINLGCSGMCFSGTTPTGTHCDTKAQCQGGLGGLETCGS
                     LAATPQLLPPSPTTAPQCYAPFLSPDLAMTRDGIRQTVLDHQRVVLALKTCGATGCGP
                     VVVNPDKIDYVGMSLGGIIGTMSAGLSPDIGSAVINVGGVGWVDILENTQTLSIRCSL
                     VNGLIDAGVLQGEKWTGGTTGLCTTDEWKQQPGYQTFAAIGRWVLDPADGANFTRFLV
                     TKKLLIQEVVGDTVVPNIATERLAALTGLLPNAKQADSFNPAQPAPSAAIADMPTVSK
                     FVRYMSDATDVFVHSSLLRPAPTDDAQKGINGTLRLQVDAATFLFANQ"
     gene            6363..7595
                     /locus_tag="VFV99_03095"
     CDS             6363..7595
                     /locus_tag="VFV99_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5058314.1"
                     /translation="MKNIRYILFAAALGSSAVASANAFNINEHDARVTGRGGATVATN
                     TTPSAMVFNPGGLAVEEGTNAAVNGTIYVAEGSYTPDGSTMKTTTDSAPALVPTGFVT
                     TRINKMFAVGIGVHFPFGLAVSWPTGHAQSDVIQDQDLKTYFITPSFGVNLYEYVPGL
                     SVGGGIDIVPATVRLQRSLPFGDTTGTVMLGGDALGIGGRFGVMYHPPSVPKLKLGAM
                     WRSDVNLDFSGKGDFDVDPTFRSQLPPDGDISTSITLPQAVTAGVAYDVLPQLQLEFD
                     AVWTNWSKFNELRIKLPDGSETVTPENYENKTTLRFGGEYTFTSVRAALRGGFIYDPT
                     PIPTTTLTAQLPDYDRKNITIGGSYYFGNYGAHLGLLWVTHGERETSDALYMPVFKAN
                     YGVDAFVASAMVSGSFGK"
     gene            complement(7607..8956)
                     /locus_tag="VFV99_03100"
     CDS             complement(7607..8956)
                     /locus_tag="VFV99_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5058315.1"
                     /translation="MRSRLVTLLALALPMVLARATQSVITFADALQTEHLGAGSLLAT
                     ATGGLNVIGIVILPMGTVFIVQSFVAQLAGAGQRAEAPRFAWYGLAIAAISALVAVAL
                     IPAIAPLLSLTHYSPSVQDEMTSYMAIRFLSVGAVVGIETLGNWYGGLGNTWMQMVAG
                     VITMVAAVFCNWLLIDGHWGAPALGVDGAAWAAVIGSWLGFAFLVLAFTRGWGGAPRP
                     GKLNLSRREFGRVVRFGLPNGFNWFLEFAAFQVFVNFVLSNLGDQVVAALNAVLAVNT
                     IGFMPAFGLASAGAILAGQAIGRGARDDVWPQVKTTLLCTMTWMGAIAVFYVTIPAHI
                     LKLFDSKGQSHQLVTVGTTMLLISAAWQLFDAVSLTLAETLRAAGDTFWTAAARLILA
                     WFVFTPAAIFVVIEWDGGPVGAMLCLVGYLALLTAALAYRFRSGAWRRIELIEPKLL"
     gene            9006..10292
                     /gene="ligD"
                     /locus_tag="VFV99_03105"
     CDS             9006..10292
                     /gene="ligD"
                     /locus_tag="VFV99_03105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5058316.1"
                     /translation="MAKAEKEIFEFDGAEVALSNPSKIYFPKAGITKREIVDYYLAVK
                     DGVLRALARRPMILKRYVDGAAGEPFYQKRAPAKRPPWLDIATFSFPSGRTADEICVN
                     HAASLVYVINLGCIEMHPHAVRSEDMDRPDELRIDLDPVPGVGWSQIIDVAKVAREVL
                     AELGLVGWPKTSGSRGAHLWVRIQPRWTFVEVRRAALAFAREVARRAPTIATAKWWKE
                     ERRGVFLDYNQNARDRTTCCAYSLRAREDARVSMPLSWDDFVVADPAEFTLRTVPAIY
                     KARGDAHAAIDDNPGSLDALFELAAKQEAEGEQDAPWPPMFAKQAGEGSRVAPSRAKG
                     AGAGRRPKLPVITIAQAKQKPDALAGLERWKAKYPDVAAKLAPEDILIDTNRGRATAW
                     YRVRINLKNVPPAEHPPQEEPDPNYDPKTEYEGWQG"
     gene            complement(10289..11053)
                     /locus_tag="VFV99_03110"
     CDS             complement(10289..11053)
                     /locus_tag="VFV99_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058317.1"
                     /translation="MTKRLLVTFAVGAVAAPTLAHAQGAMPVARPVPLYDLPPPPPEP
                     PPPVDPSQEILDEDPPPMPDLPPAPDSGPPPPPAVTVSDADRPTFTRPSPVPSLRRTG
                     FHLGTTYGFEVARDGGSSFAMGGGAQLGVEIYWPQIAIELGVAVQAHGDTTTMPDHGF
                     WEAHPMVYLKRPFKDGVWEFFAGYGASPASFHAGDFAGDEAMHVLEAGFEDRTKWMYS
                     RLFVRWYAPFDGDLFGSPTAIVGASVGLFHFSKQKH"
     gene            complement(11050..11571)
                     /locus_tag="VFV99_03115"
     CDS             complement(11050..11571)
                     /locus_tag="VFV99_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058318.1"
                     /translation="MVARTYQVAIALVLCATSAAHAEENAGGGKAFEGELGLAVVYST
                     ADLVSVGGNVGFSFGKPILDVFYVEPVLHAELGLFNAGRLAGMLRCNFVASPGIVWSV
                     GFGAGTGWKTLTDDNGNETKLSRDFREIEVALKKGGKRRFFVGLAIAFDTDEMGVESK
                     SVMLQTTLLRAGP"
     gene            complement(11565..>11827)
                     /locus_tag="VFV99_03120"
     CDS             complement(11565..>11827)
                     /locus_tag="VFV99_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058319.1"
                     /translation="DAVYPGVKKMLEDWAFKTQVKVDKANKLDAKLDAKEQKRGEKFA
                     AGRSSVADSKNPSPSNSSTSSGTTRTNGYDPTKTINDTVNSW"
BASE COUNT         2065 a         3902 c         3841 g         2019 t
ORIGIN      
        1 ggtcgacaag gcggcgacga actaccgctg gggcggcttg cagtgcaaag gccgcgactt
       61 cagcgagacg agcatcgaga tcctgttcgc cgcgtttcgc agcgagtacc aggtcaagct
      121 cgagttcgtg atcagcgact acaaggacaa gcagtatcgc tgcgtcacgg gcttcacggt
      181 gtcgaagtca tgatgcgcgt ccttcttctc ctggtcctct gcgtgccggc gcgcgcgttc
      241 gcgcttggcg aggtgtcacc gatcctgttc ctcaaccgct gcacgaacaa ctgcacggtc
      301 aacggcggcg tcgatgacgc gcgcgcgatg agctcgtcga ttccgtgcgc cgcgggtgcg
      361 tcctgcggcg gcggatcatg ctcgtgcaat ggaaccagcg cgggcacgta caccgtcgag
      421 gagttcaaga actacatggg ccagaccggc gcggcggccg acgaagagtg gaacatgatc
      481 gtgcagtgcg tgcgcgaggt gtactcgccg tacaacatcg cggtcaccga cgtgttcccg
      541 acgggcatgg ctcacaccca gggcatcgtc gccggtctac cggtcaacat tggctacggt
      601 ggcctcaata cgggtgggct cggcggcacg tcgagctgcg agcccgtcga caacgtcatc
      661 tcgttcacgt tcgcgaacat cttcacgtcg acgtcgcagc aggcgcgcgt ccgcacgctg
      721 tgttacgtgg tcgcacaaga gactgggcac tcgttcggtc tcgaccatgc gtttgaattc
      781 tccgacggcc ggtcggcgtg caccgacccg atgtcgtatc gcaatgactg cggcggccag
      841 cggttcttcc gcaacgacct cgcgcgttgc ggcgaaaacg gaccgcggcc gtgcaagtgt
      901 cccaacctgc agaactcgca tcagaagctg ctcgcgatct tcggtccggg catgccactc
      961 accgggccgc cgacgctcac cgtgtcatcc ccgacggacg gcgcgacgat ccagaacggg
     1021 acgcctgtga tcgcgacggc attcgcgcag cgcggcgtcc atcacatcga cctcttgctg
     1081 aacggctaca agtggctcac cgtcaaaggc gcaccgtttg gcgggaacgg ccagcccgag
     1141 acgacgtacc cgctcgtgct gcccagcggc gtgcccgacg gcatcatcga catcgtcgtc
     1201 aaggcgtacg acgacatcga cgccgagact gacgcgacgc tcaccgtcac caagggtgcg
     1261 ccgtgcacga ccgccgacac ctgcgccaag gggcagaagt gcgacgccgg caagtgcttc
     1321 tgggagccgc cggtcggcga gctcggcgac ccgtgcacgt acgaccagtt ctgcaagacc
     1381 ggctcgtgcg tggaaaccac gtccggccag ttctgctcga acgtttgcgt cgtcggcgtc
     1441 gacgacagct gcgggacggg cttcacgtgc gaaggcgcgg cgggaggcaa cggctactgc
     1501 gtgcccgtcg gcgcaggcga tgacacctgc tgcgggatcg gcgccaacgg caagacgtcg
     1561 gcgctgctct cgctgttcgt cgtcgggttc gtgctgcgcc gcaagcgccg ctgattactt
     1621 cgtcgccgaa ccttcggcgg ccggctcggc aggcgccgcg ggtgcctccg ccggcggcgc
     1681 gccgaccttg atctgatcag gaccgatcag atcgtgtgcg gccttcgcgc acttgccgat
     1741 gaggtcgtta tcgacgttgc tcgggccgcc gcgcacctga atgatgcact tcgtgtcgtc
     1801 gtcgaagaag cggaagcgct tcacgtcgtc tgcgccctgc gcgacgatca gcacgcacga
     1861 gtagaagccg agcgtatcgt cgtccttgtc cttcatgtcg tggcacttct ggcggaaccg
     1921 atcggcgtct tcgatcgtcc agcagaacgt cgacacgttg cagatctcgt cgccggccat
     1981 cacgccgagg cggccctctt tcttcagctt cgccttctgc tcggcatcgc tgaggcgctt
     2041 gccaccgcgc aagatcgagc acgatgccgc ggcatccggc tccgacttcg agtcggtgac
     2101 cgtgattgct tcggtctcgc cgagcacctg ctgaaactgc gtcgcgttga tcacgctcgc
     2161 gcagtcgagc ttcgctttgc cggcgacggg cgggctgatc ttcttggcga caccgccgct
     2221 cgcctccttc tctttctgtt ctttctccgc ctgcttcttg gcctcttcgg ccccttcatt
     2281 tgcacccttg ctacaggcga gcagggcgat gctcatcaga agaatcgcga gtggcgtccg
     2341 catcgcccgg aaacctaaca cgaatgtcag agcggttccc tacgatcgga tttcgatgag
     2401 gtaccaccac acgaagaaca agggcgactt cgggttggtt caagcaatgg cggacctgac
     2461 cgcgaagggc tgggcgatct tggtcccgct caccgagcac gaagcgttcg acctcgtcgc
     2521 gtgtcgcgag ggccggtttc tgcgggtgca ggtgaagtat cgcgcgatgt cgcgcggctg
     2581 cgtcacgttg cactttagat cgatcaagcc ggaacaacat ccagaagggc gttacttggg
     2641 cgagagactt tgcccagatc ccaccgacag tcctcggagt tccggcgggt ttcggcctgc
     2701 cacttgagac aatgcctttc gtgcggcaca gtcgtgagcc gcgagccgct tggctcacca
     2761 gcacccatag ttcagctgga tagaacacgg accttctaag tccggagccg caggttcgag
     2821 tcctgctggg tgcgctgcac cgtcggagct tttccgagat tcgtcggcac gtgtgctgca
     2881 cttttcgcag tgcatgaagg ccagggcgtt cgtgcgtcag ctcggcaagg aggtcgtcaa
     2941 cgacggcgtg acggatctcg gggcgatgat ggcgtactac tcgctgctcg cgctgttccc
     3001 gatgctcgtg ttcgtgatct cgctgtcgat gctcgtgctc gatcccgaga ccatccggca
     3061 gggcgccgac atggcactca acgcagcacc tcccgcggcg cgtgaggtga tcgagccgcg
     3121 cgtcaatgga ttcatcgaag cgtcacacac cggcttcgcg atcgcaggcg cgttgctcgc
     3181 gctgtggggc gcatcgcgcg gtgcggtggc gatgatgact gcgctcaaca cgatccatca
     3241 gaagaaagag acgcggtcgt ggatcaagcg gcaggcgatc gcgatcggcg tcaccgtggc
     3301 ggtggcggtg ttcatcgtga tcgcgctcgg cctgttgttt gtcggaccgt tcgtcggtca
     3361 ctacgttgcc gactacgtcg ggctcggcga cacgttcgat ctcgtgtggg gcatcggtcg
     3421 ctgggtcggc gccggtttgt tggtgatgtt cgtctgggcg atcgtttaca agtttctcgc
     3481 ggacacgaac gcgccattcc gagtgttcac tcctggcgcg atctgcggcg tgctcgtgtg
     3541 gctcggtctc agcgctggct tcagcgtgta cttgcagcac ttcaacagct acgaaaagac
     3601 gtacggcacg cttggcggcg tgatcatctt cctcacgtgg ctgtggctgt cgaacgtgac
     3661 gatgctgttc ggcgccgaga tcaatgacgt gctcgccgac ttccgcgctc acaagagcac
     3721 tgcggcagcg cagctgtcgc atgcgacgcg acgaaaagac gacggttcag cgcgccacgg
     3781 tacgggaacg cctgcgacga cctgatgatc aaaacgtagc gctaccgcac aatcatttct
     3841 gcggcgtcgt tagcgacgtg tggatgctca cgctctcttc gcgagctgcg tgatcgcacg
     3901 cagcgtacct ctcgcgaatc ctcgtgtttc tcgacgaggt tcgtatactg ctttgcgttt
     3961 cgcattcctc tacggagcaa caatgaagaa gttgatggcc tgtgcgctcg tcgcgacgat
     4021 gagcggctgt gcacccgata tcgacgtcga ccacgacgag gtcgctggtc cgacggtcga
     4081 gtttgatccc gcgcgatcgc aagcaaccgg cgcgcgcttc attccgtttc cgaacgatct
     4141 cgttcgcgat ccgacgacgg gcaaagtgaa cttgccgccg caggcgtgcg aaacgccggc
     4201 gcagaaggcg acgcgcgaga acattctcaa caagctggac ggcttcggca cgtacgagac
     4261 cgcgatgcag gtgacgttca cgaacgacgt ggacgaagca tcgcttgcgg gtcacatcgt
     4321 gatgttccag ctgaccgacg agggcgtgcc gatcatgccg tcggcagcga cgcagccgat
     4381 tccggtcacg gtcgtgcgca ccggcttgtc gccgcgcttc gagaagggca acgacttctg
     4441 cactccaatg gatgcgaaca acgtcccgtc gctcacgatg gtgccgtcgg tgccgctcaa
     4501 gcagaagagc acgtacttcg tcgcgctcct gaagggcatc accgacacca gcgggaccga
     4561 gttcggcgcg tcgccgacgt ggggtcttgt cgcgagccaa acacctccgg tcacgctcga
     4621 tgccgcgggc aacgtcgtcg ccgatcgtac gccgctcgat ccatcgatcc ccgagcagcg
     4681 cgcgcagctc gtcgcactcg caggcttgtg gcagctgcat cagccgggca tcgagttcgt
     4741 gcaggcgacc acgggcgccg cgcgcagtga catcctcgtc ggcttccagt tcacgacgca
     4801 gaccacgacc gatcagctcg atccgagtgt gacgggcagt cctgcgaaca agctctcgac
     4861 ggtcggcgtg ctgatgacgg agtcggtgac cggcaagttc ggtccgtact cggcgctgtg
     4921 cggcgcagag gcgacgaccg cacccgcgca gtgcttcttg aagctcgcgc tcggcggttg
     4981 ctcgccgctg acgacgggct gcgatgcgaa caactacaac gcaggcaacg ttgcgtgcca
     5041 gttcctctac aactgtgccg cggtcggcga cgtcattggt ggtggcattc tcaccgcgaa
     5101 ctaccagacg cagatcgaca acccgttcga tgcgaagcat ccgatccagg gcgcgtggag
     5161 tgatccgatc aaccccgagc agcaggcgac gctgacgctc gagacgatca tcgtgctgcc
     5221 cgcgggcacg ccgccggcga cgggctggcc gaccattctg ttcagccacg gcctcggcag
     5281 ctccaaggag agcgtgttcg cgatcgcggg ccgcttcgcc ggtgctggct tcgcgacggt
     5341 cgcgatcgat accgcggcgc acggtagccg cgcggtccgc atctccgatg acatcaacct
     5401 cggctgtagc ggcatgtgct tctcgggcac gacgccgacc ggcacgcact gcgacacgaa
     5461 agcgcagtgt cagggcggtc tcggcggcct cgagacctgc ggcagcttgg ccgcgacgcc
     5521 gcagctcctc ccgccgtcgc cgacgaccgc gccgcagtgt tacgcgccgt tcttgtcgcc
     5581 tgacctcgcg atgactcgcg atggtattcg ccagaccgtg ctcgatcacc agcgcgtcgt
     5641 gctggcgctc aagacgtgcg gcgccaccgg atgcggtccg gtcgtcgtca acccggacaa
     5701 gatcgactac gtgggtatgt cgctcggcgg gatcatcggc acgatgagcg cgggcctgtc
     5761 gcccgacatc ggatcggccg tcatcaatgt cggcggcgtc ggttgggtgg acatcctcga
     5821 gaacacccag accctctcga ttcgctgctc gctcgtcaac ggcctcatcg acgccggcgt
     5881 gctgcagggc gagaagtgga ccggcggcac gacgggcctg tgcaccaccg acgagtggaa
     5941 gcagcagccc ggctaccaga cgttcgccgc gatcgggcgc tgggtgctcg acccggccga
     6001 cggcgcgaac ttcacgcgct tcctcgtcac caagaagctg ctgatccagg aagtggtcgg
     6061 cgacaccgtg gtgccgaaca tcgcgaccga acgcttggcg gcactgaccg gcctgttgcc
     6121 gaatgcgaag caagccgatt cgttcaaccc ggcgcagcct gcgccgtcgg cggcgatcgc
     6181 ggacatgccg acggtcagca agttcgtgcg gtacatgagc gacgcgaccg acgtcttcgt
     6241 ccactcgtcg ttgctccgcc ccgcaccgac cgacgatgcg cagaagggca tcaacggcac
     6301 gctgcgtctg caggtcgatg ccgccacgtt cctctttgcc aaccagtagc caggacctga
     6361 ccatgaagaa catccgttac atcctcttcg cggcggcgct tggctcctcg gctgtcgcgt
     6421 ctgcgaacgc attcaacatc aacgagcacg acgcacgcgt caccggccgc ggtggcgcga
     6481 ccgtggcgac gaacacgaca ccgtcggcga tggtgttcaa ccccggcggc ctcgcggtcg
     6541 aggagggcac gaacgccgcc gtcaacggca cgatctacgt cgccgaaggc agctacacgc
     6601 ctgacggcag cacgatgaag acgacgaccg attcggcgcc cgcattggtg ccgaccggat
     6661 ttgtcaccac gcgcatcaac aagatgttcg cggtcggcat cggcgtgcac ttcccgttcg
     6721 gtctcgcggt gtcgtggccg accggtcacg cacaatcaga cgtgatccag gatcaggatc
     6781 tcaagacgta cttcatcacg ccgagcttcg gcgtcaacct gtacgagtac gtgccggggc
     6841 tctcggtcgg cggcggcatc gacatcgtgc cggccaccgt ccggctccag cgctcgcttc
     6901 cgttcggcga cacgaccggc acggtgatgc tcggcggtga tgcactcggc atcggcggcc
     6961 gcttcggcgt catgtatcac ccgccgagcg tgccgaagct caagctcggc gcgatgtggc
     7021 gcagcgacgt caacctcgac ttctccggca agggcgactt cgacgtcgac cccacgttcc
     7081 gctcgcagct gccgcccgac ggcgatatct cgacgtcgat cacgttgccg caagcggtga
     7141 cggccggcgt cgcctacgac gtgctgccgc agctccagct cgagttcgac gcggtgtgga
     7201 cgaactggtc gaagttcaac gagctccgca tcaagctccc cgacggctcc gagaccgtga
     7261 cgccggagaa ctacgagaac aagaccacgc tgcgcttcgg cggcgagtac acgttcacgt
     7321 cggtcagagc ggcgctgcgc ggcggtttca tctacgaccc gacgccgatt ccgaccacga
     7381 cgctgaccgc gcagctcccc gactacgacc gcaagaacat cacgatcggt ggtagctact
     7441 acttcggcaa ctacggcgcg cacctcggcc tgctctgggt cacgcacggt gagcgcgaga
     7501 cgtcggacgc gctgtacatg ccggtgttca aggcgaacta cggcgtcgac gcgttcgtcg
     7561 cgtcggcgat ggtcagcggc agcttcggca aatagctaga agcccgctag agcagcttcg
     7621 gctcgatcag ctcgatccgc cgccaggcgc cgctgcgaaa ccggtaggcg agggcggcgg
     7681 tgagcagcgc gaggtagccg acgaggcaca gcatcgcgcc gaccggcccg ccgtcccact
     7741 cgatcacgac gaatatcgcg gccggcgtga acacgaacca ggcgaggatg aggcgtgcgg
     7801 cagcggtcca gaacgtgtcg ccggccgcgc gtagcgtctc ggcgagcgtg agcgagaccg
     7861 cgtcgaacag ctgccatgcg gcgctgatga gcagcatcgt cgtaccgacg gtgacgagct
     7921 ggtgcgattg gcccttgctg tcgaacagct tcaggatgtg cgcggggatc gtgacgtaga
     7981 acaccgcgat cgcgcccatc cacgtcatcg tgcaaagcag cgtcgtcttg acctgcggcc
     8041 acacgtcgtc gcgcgcgcca cggccgattg cctggcccgc gaggatcgcg cctgccgacg
     8101 cgagcccgaa tgcgggcatg aagccgatcg tgttcaccgc gagcacggcg ttgagcgcgg
     8161 cgacgacctg atcgccgagg ttcgacagca cgaagttgac gaacacctgg aacgcggcga
     8221 attcgaggaa ccaattgaag ccgttcggca gcccgaagcg tacgacgcgc ccgaattcgc
     8281 ggcgcgacag gttcagcttg cccgggcgcg gcgcgccgcc ccagccgcgc gtgaatgcga
     8341 gcacgaggaa cgcgaagccg agccagctgc cgatcacggc agcccacgcg gcgccgtcga
     8401 cgccgagcgc aggtgcgccc cagtggccgt cgatgagcag ccagttgcag aacacggccg
     8461 cgaccatcgt gatgacgccc gcgaccatct gcatccacgt gttgccgagg ccgccgtacc
     8521 agttgccgag cgtctcgatg ccgacgaccg cgccgaccga caggaagcgg atcgccatgt
     8581 agctcgtcat ctcgtcctgc acggagggcg agtaatgcgt gagcgacagc agcggcgcga
     8641 ttgcaggaat gagcgcgacc gcgacgagcg ccgagattgc ggcgatggcg aggccatacc
     8701 aggcgaaccg cggcgcctcc gcgcgttggc cggcgccggc aagctgcgcc acgaagctct
     8761 gcacgatgaa cacggtgccc atcggcaaga tcacgatgcc gatgacgttg aggccgccgg
     8821 tcgccgtggc caatagcgag cccgcaccga ggtgctcggt ctgcaacgcg tcggcgaacg
     8881 tgatcaccga ttgcgtggcg cgcgcgagga ccatcggcag cgccaacgcg agcaacgtga
     8941 cgagccggga ccgcatgagg gcggtgaaat gtacgcgcaa acgccgggca tgcaaggatg
     9001 cacgcatggc caaggccgag aaggagattt tcgaattcga tggcgccgag gtggcgctgt
     9061 cgaatccgag caaaatctac tttcccaagg ccgggatcac gaagcgcgag atcgtcgact
     9121 actacctcgc cgtgaaggac ggcgtactgc gcgcgttggc gcgccggccg atgatcctga
     9181 agcgctatgt cgacggcgcc gcgggcgagc cgttctacca gaagcgtgcg ccggcgaagc
     9241 gaccgccgtg gctcgacatc gcgacgttct cgtttccatc cggccgtacc gccgacgaga
     9301 tctgtgtcaa tcacgccgca tcgctcgtct acgtgatcaa cctcggctgc atcgagatgc
     9361 acccgcacgc ggtccgcagc gaggacatgg atcgtcccga cgagctgcgc atcgatctcg
     9421 atccagtccc aggtgtcggc tggtcgcaga tcatcgatgt cgcgaaggtc gcccgcgagg
     9481 tgctcgcgga gctcgggctc gtcggttggc cgaaaaccag tggctcgcgc ggcgcgcacc
     9541 tgtgggttcg cattcagccg aggtggacgt ttgtcgaggt gcggcgtgca gcgctcgcat
     9601 tcgcgcgcga ggttgcgcgg cgtgcgccga cgatcgcgac ggcgaagtgg tggaaggagg
     9661 agcggcgcgg cgtgttcctc gactacaacc agaacgcgcg cgatcgcacg acctgctgcg
     9721 cctactcgct gcgcgcgcgc gaggacgcgc gagtgtcgat gccgctgtcc tgggacgact
     9781 tcgtcgtcgc agatccggca gagttcacgt tgcgtacagt gccggcgatc tacaaagcac
     9841 gcggcgacgc gcacgctgcc atcgacgaca accccggctc gctcgacgcg ttgttcgagc
     9901 tcgccgccaa gcaagaagca gaaggcgagc aggatgcacc gtggccgccg atgttcgcga
     9961 agcaggccgg cgagggcagc cgcgttgcac caagccgcgc gaaaggcgcc ggcgcaggtc
    10021 gtcggccgaa gctaccggtg atcacgatcg cgcaggcaaa gcagaagccc gacgcgctcg
    10081 ccggcctcga gcgctggaag gcgaagtatc ccgacgtcgc ggcgaagctc gcacccgagg
    10141 acatcttgat cgatacgaac cgcggccgcg cgaccgcctg gtaccgcgtg cggatcaatc
    10201 taaagaacgt gccgcccgcc gagcatccgc cccaggaaga accggatccg aactacgatc
    10261 cgaagaccga atacgagggc tggcagggct agtgcttttg tttcgagaag tgaaataggc
    10321 ccacgctcgc gccgacgatc gcagtcggtg agccgaatag atcaccgtcg aacggcgcgt
    10381 accagcgcac gaacaaccgc gagtacatcc acttcgtacg gtcctcgaag cccgcctcga
    10441 gcacgtgcat ggcctcgtcg ccggcgaagt cgcccgcgtg aaacgacgcc gggctcgcgc
    10501 cgtagccggc gaagaattcc cagaccccat ccttgaacgg tcgcttgaga tacaccatcg
    10561 ggtgcgcttc ccaaaacccg tggtccggca tcgtcgtcgt atcgccgtgt gcttgcaccg
    10621 ccacgccgag ctcgatcgcg atctgcggcc agtagatctc gacgccgagc tgggcgccac
    10681 cacccatcgc gaagctcgac ccgccgtcgc gcgcgacctc gaagccgtac gtcgtgccga
    10741 ggtgaaagcc ggtgcggcgc agacttggca ccggtgacgg acgcgtgaac gtcggccggt
    10801 ccgcgtcgct caccgtcact gcgggcggtg gtggcggacc gctgtcggga gccggcggca
    10861 gatccggcat cggcggcgga tcctcgtcga ggatctcctg actcggatcg accggcggcg
    10921 gcggctccgg cggcggcggc ggcagatcgt agagcggcac cggccgcgca accggcatcg
    10981 cgccttgggc gtgcgcaagc gtgggcgcgg cgaccgcgcc gaccgcgaag gttacgagca
    11041 agcgcttcgt cacggcccgg ctcgcaatag cgtggtttgc aacatcacgg atttgctctc
    11101 gacacccatc tcgtcggtgt cgaatgcgat cgcgaggccg acgaagaagc ggcgcttgcc
    11161 acctttcttg agcgcgacct cgatctcgcg aaagtcgcgc gacagcttcg tctcgttgcc
    11221 gttgtcgtca gtcagcgtct tccaaccggt cccggctccg aatccgactg accacacgat
    11281 gcctggcgat gccacgaaat tgcagcgcaa catgcccgcg agccggccag cgttgaacag
    11341 accgagctca gcatgcagta ccggctcgac gtagaacacg tcgaggatcg gcttgccgaa
    11401 cgagaacccg acgttgccgc caacgctcac gagatcggcg gtgctgtaca cgaccgcgag
    11461 cccgagctcg ccttcgaacg ccttgccgcc accggcgttc tcctcggcat gtgcggcgct
    11521 cgtcgcgcag agcaccagcg cgatcgctac ctgataggtg cgcgctacca cgagttgacg
    11581 gtgtcgttga tggtcttcgt gggatcgtac ccgttggtgc gagtggttcc cgagctcgtc
    11641 gacgagttgc tcggtgacgg attcttgctg tcggcgacgc tgctgcggcc agcggcgaac
    11701 ttctcgccac gcttttgttc cttcgcgtcg agcttcgcgt cgagcttgtt tgccttgtcg
    11761 accttcacct gcgtcttgaa cgcccagtct tcgagcatct tcttcacgcc ggggtacacc
    11821 gcgtcgt
//
LOCUS       DASUFX010000080         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1541811, whole genome shotgun sequence.
ACCESSION   DASUFX010000080 DASUFX010000000
VERSION     DASUFX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541811"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3396)
                     /gene="smc"
                     /locus_tag="VFV99_03125"
     CDS             complement(<1..3396)
                     /gene="smc"
                     /locus_tag="VFV99_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5058320.1"
                     /translation="MTQAFTRDVEVTRRLYRSGESEYLIDGEVCRLRDVHELLMDTGL
                     GAKAYAIIEQGKIGMILSSRPTDRRQLIEEAAGITKYRSRRRAAELKLEAAQQNLVRI
                     DDIVFEVEKQRGTLKRQAAKARRYKRLRDEYRRWEKVLFARKYRELAASIEGSRNKLG
                     LAREREAAAAARLAEVETELGRLRLSQTEADNSASAIREDAHARELDINRRQQQLEFN
                     RHQADTLDVRGTEIEAEIRDLEARREPARIAMESRRSAQTDAERSRDEAADVMASANA
                     AYAAAQQQIEGLDAHVEGARQGVYMSANSVMAVRHALEHTASQHERVGEALAKLDVEE
                     DDLRREQDRVTYERTAAIESLSRAQDALESIRLQHAAKKSELASARAEHEGRAREVRT
                     REQELAAVEARLASLQELATSRADFGDAARMVLVQANGHVGQQGAVADYVDVDRRYER
                     AVEACLGELLEHVIVERHDQAAAGLSLVRQHDAGRCGFVVVDPGSNGYHPREVLRMPG
                     IVPVSDVLHVQGPHAVTIHKVLPEAYVADTFEQAVSFSRQTSAPIATLEGDVLRGPHL
                     VTGGAKVESRGILATRREIKELLERVAGDREQLTRLAADVAQLELTIAQATGAITGLQ
                     AEEHAQEKAIVAYEAQRDRADEEQARLSRKAEVIAVERRTAEEERAALEARRSEADAS
                     SVRLESEQRAAEERLAEAQRALADARDTAGTLGAQAAEARANHAALVERSAALAAEVL
                     RLEEGARELELRIGAHHAELQQTHTRRESLLQSIVDGQRTLDEDIQTLVGKRDELRVA
                     DEHAATLRAAVDAQDSVIREARGAVEAIRSEATDLEVQCATAQSDLTHLAQTCMDAVQ
                     VSLDEVLAEVEQMEQAGQATPDAAAITAEEPDPEAEDEMPTEGAAYAATSDAAVVASE
                     PEHATAAAQPPASMTAEEAITRLKAKIDRLGPVNMMAIEQFDELEQRHVFLTTQRRDL
                     VESIAQTSQAIARIDETTKARFTEAFTAIQANFQVTFSTLFGGGRAGLTLLDENDPLE
                     SGIDIVASPPGKRLQSVMLLSGGEKALTAIALMFAIFKYKPSPFCLLDEIDAPLDDAN
                     VHRFIEMLRGMLDRTQFILITHNRQTMEIANRLYGVTMEEPGVS"
BASE COUNT          516 a         1153 c         1065 g          677 t
ORIGIN      
        1 cgacacaccc ggttcttcca tcgtcacccc gtacaaccga ttggcaatct ccatcgtctg
       61 gcgattgtgg gtgatgagga tgaactgggt gcgatcgagc atgccgcgca acatctcgat
      121 gaaccgatgc acgttggcat catcgagcgg cgcatcgatc tcgtcgagca gacagaacgg
      181 gctcggcttg tatttgaaaa tcgcgaacat caacgcaatt gccgtcagcg ccttctctcc
      241 acccgacagc agcatcacgc tctgcaaccg cttccccggc ggtgatgcca cgatgtcgat
      301 gccgctttcg agcggatcgt tttcatcgag cagcgtcagg ccggcgcgac cgccgccgaa
      361 gagcgtgctg aaagtcacct ggaagttcgc ctggattgcg gtaaacgctt ccgtgaagcg
      421 ggccttcgtg gtttcgtcga tgcgggcgat ggcctgcgac gtctgcgcga tcgactcgac
      481 cagatcgcgg cgctgcgtgg tcaggaacac gtgacgctgc tcgagctcgt cgaactgctc
      541 gatggccatc atgttcaccg gaccgaggcg atcgatcttc gctttgagac gcgtgatcgc
      601 ttcttccgcc gtcatcgatg ccggcggctg tgccgcagcg gtggcatgct caggttcact
      661 cgccacgaca gcggcatcac tcgtggccgc gtaggcggcg ccttccgtgg gcatctcgtc
      721 ttcggcttcg gggtccggtt cctcggccgt gatcgccgcg gcatcgggcg tggcctgccc
      781 cgcctgttcc atctgctcga cttcggcgag gacctcgtcg agagacacct gcacggcatc
      841 catgcatgtc tgcgcgaggt gcgtgagatc gctctgcgcg gtcgcgcact ggacctcgag
      901 atcggtggcc tcgctgcgaa tcgcttcgac cgctcccctg gcctcgcgaa tgaccgagtc
      961 ctgggcgtca accgctgcac gcagcgttgc cgcatgctcg tcagcgacac gcagctcgtc
     1021 gcgcttgccg accagtgtct gaatgtcttc atcgagcgtg cgctgtccat ccacgatcga
     1081 ttgcaacagc gactcacgac gagtgtgggt ctgttgcagc tcggcgtgat gcgcgccgat
     1141 gcgcagttcg agctcccgcg caccttcctc gaggcggaga acttcggcgg cgagcgccgc
     1201 cgagcgctcg acgagtgccg cgtgattggc acgcgcttca gcggcctgtg cgccaagcgt
     1261 tcctgctgtg tcgcgcgcat cggccaacgc tcgctgcgcc tcggcgaggc gttcttccgc
     1321 tgcgcgctgt tcgctctcga ggcgcacact cgaggcgtcg gcctcactgc gcctggcttc
     1381 gagcgcggcg cgctcctctt ccgccgtgcg gcgctcgacg gcaatcacct cggctttgcg
     1441 cgacaaacgc gcctgttcct catcggctcg atcgcgctgc gcttcgtagg cgacgatcgc
     1501 tttttcctgc gcgtgctcct cggcctgcag gccggtgatc gcgccggtgg cttgggcgat
     1561 ggtcagctcg agctgcgcga cgtcggcagc caaccgcgtg agctgctcgc gatcgccggc
     1621 gactcgctcg agcaattcct tgatctcgcg acgtgtcgcg agaatgcccc gcgattcgac
     1681 ttttgcgccg ccagtcacca gatgcggacc gcgcagcacg tcaccttcga gcgtggcaat
     1741 cggcgcggac gtctggcgcg agaacgacac cgcctgctcg aaggtgtcgg cgacgtaggc
     1801 ttcgggaagg actttgtgga tcgtgacggc gtgcgggcct tgcacgtgga gcacatcgga
     1861 taccggcacg atgcccggca tgcgcagcac ctcacgtggg tgatacccgt tcgaacctgg
     1921 atcgaccacg acaaacccac agcggccggc gtcgtgctgg cgaaccagtg acaagccggc
     1981 ggcggcctgg tcgtgacgct cgacgatgac gtgctcgagc aattcaccga gacacgcttc
     2041 gaccgcgcgc tcgtaacgac gatcgacatc gacatagtcc gcgacagcgc cctgctggcc
     2101 gacgtgaccg ttggcctgca cgagcaccat ccgtgcagcg tcgccgaaat cagcgcggct
     2161 cgtcgcgagc tcctgcaatg aggccagccg tgcttcgact gcggcgagct cctgctctcg
     2221 cgtccgtacc tcacgcgcgc gaccttcatg ttccgcacgc gcgctggcca attcggattt
     2281 cttcgccgcg tgctgcaggc ggatcgattc gagcgcgtcc tgcgcacggc tcagcgattc
     2341 gatcgcggcg gtccgctcgt aggtgacccg gtcctgctcg cggcggagat cgtcttcctc
     2401 gacatccagc ttggcgagcg cctcgcctac tcgttcgtgc tgcgacgccg tgtgctcgag
     2461 cgcatggcgc acggccatga ccgagttcgc cgacatgtac acgccctggc gtgctccctc
     2521 gacgtgcgcg tcgagtcctt cgatctgctg ctgcgccgcg gcgtaggcgg cattcgccga
     2581 tgccatcaca tcggccgcct cgtctcgcga gcgctcggca tcggtctgcg cactgcggcg
     2641 cgattccatc gcaatccgcg ccggctcgcg acgggcttcg agatcgcgaa tctctgcttc
     2701 gatctcggtt ccacggacgt cgagcgtgtc ggcctgatgg cgattgaatt cgagctgctg
     2761 ctggcggcga ttgatgtcga gctcacgcgc atgcgcgtct tcgcgaatcg ccgatgcgct
     2821 gttgtctgct tcagtctgcg agagacgcag acggccgagc tcggtttcaa cttcagccag
     2881 ccgtgcggcg gcggcggctt cgcgctcgcg tgcaagaccg agcttgttac gcgaaccctc
     2941 gatactcgcg gccagctcgc gatacttgcg cgcgaagagc actttctccc accggcgata
     3001 ctcgtcgcga agccgcttgt agcggcgtgc cttcgcggcc tgacgtttga gtgtgccgcg
     3061 ctgcttctcc acttcgaaga cgatgtcgtc gatgcgaacc aggttctgct gcgcggcctc
     3121 gagcttcaat tcggcggcgc gacgacgcga tcgatatttg gtaatgccgg cggcctcttc
     3181 aatcaactga cggcggtcag tcggtcgaga gctgagaatc atgccgatct tgccctgctc
     3241 gatgatcgcg taggccttcg cacccagccc cgtatccatc agcagttcgt gcacatcgcg
     3301 cagtcggcag acctcgccgt cgatcagata ctcgctttca cccgatcgat acagccgtcg
     3361 cgtgacttcg acgtcgcgcg tgaacgcctg cgtcaactcc tcgagatcga c
//
LOCUS       DASUFX010000081         9385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_290426,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000081 DASUFX010000000
VERSION     DASUFX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290426"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFV99_03130"
     CDS             <1..787
                     /locus_tag="VFV99_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5058321.1"
                     /translation="HLAEHSPIGTFEALDYALWASSTFGDALDRLVRFLRIIGDDLTL
                     HVTRAGKWARVHRVVVHDQRHRAECFLAILVLRGRELLGRPLRLREVRFTHRRPADIR
                     AHRRLFHCPIRFGSSVTELVFDVNQLDAPVRSARPGLAAVLDRHLRDLLSRLPQRGSF
                     VHRVHQSIARTLHAGRPSLKTTARALHMSSRTLQRHLQDVGVTHRQIVEDVRRDLAER
                     LLATRRMSITEVAFLLGFEDVSGFRRAYRQWTGMNPSRARAKI"
     gene            787..1803
                     /locus_tag="VFV99_03135"
     CDS             787..1803
                     /locus_tag="VFV99_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5058322.1"
                     /translation="MARFAPIIGAFVRFCPHQLPNLGVMQAVVVLTCGDPARRAALEA
                     ALAEQGLGATDSFDGSWSLHVIDNAVGVTMRSENEVLARVSVAISPEEVAALARLAQE
                     VMMARAKIRSFDDAESLGLLALMVAHDARNALVPMVVAADALSDLEHARTVELAQVLV
                     DGCRHLTSILRRITTVEQNRRPEVVDVNSMIRNYSATLSAMLAPRVSLVTSLDAAVPT
                     VAIDVADLERMLLNLVANAYEASPEGAEVVVSTASTRKRDGLPLGDWVVITVEDRGRG
                     MDATTRDSASRLFYTTKSPSLGAGLGLSSVARLARSAGGHLLIESEPGSGTKVGIWLP
                     PAER"
     gene            complement(1805..2011)
                     /locus_tag="VFV99_03140"
     CDS             complement(1805..2011)
                     /locus_tag="VFV99_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058323.1"
                     /translation="MSNVERTTEAPSEIETLETLETVTGGCSFGGCYGGGCGGRIAYR
                     SSGFGFDPMFMLLAMTLMSNSSTK"
     gene            2185..2610
                     /locus_tag="VFV99_03145"
     CDS             2185..2610
                     /locus_tag="VFV99_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5058324.1"
                     /translation="MREIELSDYRALADFRHQLRRFIAFSETAARGVSLEPRQHQVLL
                     ALRGLPEGVEPTVQVLADRLVLRHNTVVELLDRLEAEGLIRRGRAAEDRRRAVITITA
                     RGLELLSKLSRSHLDELRSLAPALVGALNSALRASRSRV"
     gene            2607..4259
                     /locus_tag="VFV99_03150"
     CDS             2607..4259
                     /locus_tag="VFV99_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5058325.1"
                     /translation="MKELGDFTATRRMIPITGVAIGIGVLAAFVALALLRLIGLFTNL
                     CFFQRWDTTLVAPAGHHLGPFVMLVPVAGGLIIGLMAKYGSEKIRGHGIPEALESILL
                     RGSRVETKLAILKPLSAAISIGSGGPFGAEGPIIMTGGAFGSLVAQRFHLTAAERKTL
                     LVAGSAAGMAATFGTPLAATLLAVELLLFEWKPRSFVPVALACASAMATRHFLFDSAP
                     LFPVNAPVSGDDLAVLLGCIGAGLGAGVLSAAMTASVYFFEDRFLRLRMHWMWWPALG
                     GLAIGIGGLIEPRALGVGYDVIGDLLHGHLALAVIVTLVLTKWAIWAVSLGSGTSGGV
                     LAPLLMLGASLGALLVPIMPSAPTGFWQLIGMGAILGGTMRSPLTGILFAAEVSGDFN
                     ALLPLSVAVVVAHAFTVLTLRRSVLTEKVARRGFHITREYEIDPLEVLFVREVMRPEV
                     TADTKGEVAFVQETLRTATLRMAKHGVIALRVVNEQSQPVGTIVLEDVLGARKRHLEE
                     ETRREALRPLPSWMPIPQWVPLVPPRERSRSLAVEERSRHDE"
     gene            complement(4221..7487)
                     /locus_tag="VFV99_03155"
     CDS             complement(4221..7487)
                     /locus_tag="VFV99_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058326.1"
                     /translation="MAGSAPTADELLAHTEWLSRVARALVGDASADDVVQDTYEVALA
                     KPPKRAGPLRPWLGGVARNVARMATRGRVRREQREQAAPVHDEVPSPEELVARVQMQQ
                     KVGRLVLELHEPLRSTLLLRFFEGMNASEIARAQGVPAATVRSRIKDALDRIRAALDE
                     EHGNDRTAWAGLLAPLPAAMPHGTAVFAGGLLVKTSIKVLLVALAAAVLVAGTRFAGL
                     WGGGEKDKPAVTAKTVPPTKPLPKPTEPAPTNGSTRELPTIHDDDPKGTLRLEGQVID
                     EHDAPVAHAMVAIDANPPMVVETESDGGFVFDGLIRRDYRIEATAGDRYAGPARLRLA
                     DKPEPVTLRMHKGGTVEVAITERQGGTPVKGAEVELRSTLTWKATTNADGIAQLRGVG
                     AGWAPLVARAKGYSQAAMMLGTSGNGDTTEHVALSLAHGAALSGRVIDEKGKPIANAR
                     VVPTFASEPLPVVDPRRDGVLTGADGAFSLPALSAGTWRITATAGDFAPATTMPLTVD
                     GEHAKSGVEIQLTSGAIVRGVVQDSSGKPVAAADVSVVVHGFVPWRARRQAFTDADGK
                     FAISGLARRPMDVVAWHESGASAIVAVDLAAKREQDVALKLDVSGAIAGTVVDKAGQP
                     IADAQVIAEPEFTGGTADRAVWVVRGVQETVSDQSGAFRFAGLPDGSYRVRAARPGAS
                     EAALELSQGVAAKPNGAAIKIVVPADGRVIGKVQLADGKAPIAFTAALGGTNPLPFAA
                     KDGAFALPAAAGTYPLTISGPGFVTTTKQATITEGKDADLGTITVAAGRSLSGRVIDE
                     HGAPVAKATVAAGMLLTGGGAELYIKDESIMAQDTETDENGRFVMQGFTPGPITVVAG
                     KSAVGRSASIQIPAGPDSATVDLVLSGTSSLEGKVVRAGQPLPDTVVIANPIGAVASN
                     FFVTTGPDGTFALDALAPGSYLISPMLGGGGNRPKDMYFRRVEVTAGAKTKVELDATP
                     GTITLTLSVKTDKGAPVPVAQLITFQAAANPQTLAELRDGTRFPFSEQVMPIYMRTVV
                     DGAATIEGMKAALHTACAVLGDPRDPSTAKFKCSQFKLTGAAKQTASLVVPAAFLDGK
                     "
     gene            complement(7544..7975)
                     /locus_tag="VFV99_03160"
     CDS             complement(7544..7975)
                     /locus_tag="VFV99_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5058327.1"
                     /translation="MAAVLGLVADVLGEFGLEFGKGSATDAELHGLPASYTTRGGAFW
                     IALRDGELLGTCGVFPVAPGIYELRKMYLRGASRGLGLGKQLLEVAVAWSRKRGGTHM
                     VLDTVEQMTRAIAFYEANGFVRDDAQIRGARCTRGYVRVLA"
     gene            complement(8005..8373)
                     /locus_tag="VFV99_03165"
     CDS             complement(8005..8373)
                     /locus_tag="VFV99_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5058328.1"
                     /translation="MKHSPGFLKLVDDAKRNIRETTVSAVAERLKRGDKFHFVDTRED
                     DEWRAGHIPGAIHLGKGVIERDIETKIPNHGDEIILYCGGGFRSALAAEALQKMGYTN
                     VASMDGGIRAWRAQGLPEDK"
     gene            8423..>9385
                     /locus_tag="VFV99_03170"
     CDS             8423..>9385
                     /locus_tag="VFV99_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5058329.1"
                     /translation="MEVIPVHIAVLTIADDAASADRATAQAISERATAAGHQIVDEEV
                     VADTESAIRDQLVRWIAEDNIDVVVVAASVESEAASAALAPLVHQTLPGFTDLFRWLT
                     FQEIGASAMLSAAEAAQCQSTFVFVLPAHEGAVRAAMDKLILPQLDVRTKPKNLVSQM
                     PRLKDAAKKFADANKSQRVSVPAAAVAPTPSAPAPVAIPAAAYAPHPTARPETEPEAV
                     PKPIAPEKTEGGHGLPPKLPAGVAPAVARSKPRTANTIARKTDDPPTKEIDLANLEKQ
                     IQLSNQDGPTKKIDLSAKTKVVDMSAHQAKTRVVDSGRLPRVPPG"
BASE COUNT         1433 a         3133 c         3153 g         1666 t
ORIGIN      
        1 gcacctcgca gagcattcgc cgatcggcac gttcgaggca ctcgattacg cgctatgggc
       61 aagctcgacg tttggcgatg cactcgaccg cctggtccgc ttcctgcgca tcatcggcga
      121 cgatctcacg ctgcacgtga ctcgcgcggg caaatgggcg cgtgtgcatc gcgtcgtcgt
      181 gcacgatcaa cgtcaccgcg ccgaatgttt ccttgccatt ctcgtgctgc gcggccgcga
      241 attgctcgga cggccgctcc gcttgcgcga ggtgcggttt acgcatcgac gccccgcaga
      301 catccgtgct catcgccgcc tgtttcattg tcccatccgg tttggctcgt cggtcaccga
      361 gctcgtgttc gacgtcaatc agctcgacgc accggtgcgc agcgcgcggc cgggcctcgc
      421 agccgtgctg gatcgccatc tgcgcgattt gctttctcgt ctgccgcagc gcgggtcgtt
      481 cgtgcatcgc gtgcaccagt cgatcgcacg cacgctgcat gcaggccgcc cgtcactgaa
      541 gaccaccgcg cgcgctctcc acatgtcgtc acgcacgttg caacgtcatc tccaggacgt
      601 tggcgtcacg catcgtcaga tcgtcgagga cgtgagacgc gaccttgcgg agcgattgct
      661 cgcgacgcgt cggatgtcga tcaccgaggt cgcgtttctg ctcggcttcg aagatgtcag
      721 cggattccgc cgtgcgtacc ggcagtggac gggtatgaat ccatcgcggg cgcgcgcgaa
      781 gatctagtgg cgcgcttcgc gcccattatt ggcgcgtttg tccgcttttg cccgcaccag
      841 ttgccgaacc tcggcgtgat gcaagctgtt gtggttctga cctgcggcga ccctgcccgt
      901 cgcgcagcgc tcgaagccgc gcttgccgag caagggctcg gcgcgacgga ttcgttcgac
      961 ggatcgtggt ctctgcacgt gatcgacaat gcggtcggcg tgacgatgcg gagcgagaac
     1021 gaagtcctcg cccgcgtgtc ggtcgccatt tcgccggaag aagtcgctgc gttggctcgg
     1081 ctcgcgcagg aagtgatgat ggcacgcgca aagatccgat cctttgatga cgccgaatcg
     1141 ctcggtctgc tcgcgctgat ggtcgcgcac gacgcgcgca atgcgctcgt gccgatggtc
     1201 gttgcggcgg acgcgctgag cgatctcgaa cacgcacgca ccgtcgagct cgcgcaggtc
     1261 ctcgtcgacg gctgccggca tctcacgtcg atcctgcggc ggatcacgac ggtcgagcag
     1321 aatcgtcggc ccgaggtcgt cgacgtgaac tcgatgatca ggaactactc ggctacgctc
     1381 agcgcgatgc tcgctccgcg ggtgagcctc gtgaccagcc tcgatgctgc agttccgacc
     1441 gtcgcgatcg atgtcgcgga tctcgagcgc atgctgctca acctcgtcgc caacgcgtac
     1501 gaggcaagcc ccgagggcgc tgaggtcgtc gtctcgaccg cgtcgacgcg gaagcgcgat
     1561 ggcttgccgc tcggcgattg ggtcgtgatc acggtcgaag atcggggcag ggggatggat
     1621 gcgacgaccc gcgatagtgc gtcgcgactg ttctacacga cgaagtcacc gagcctcggt
     1681 gcaggtctcg gcttgtcgag cgtggcgcga ttggcacggt ccgctggcgg gcatctcctg
     1741 atcgagagcg agcccggcag cggcacgaag gtcggaatct ggttaccacc cgccgagcgg
     1801 tgagctactt cgtgctgctg ttcgacatca gtgtcatcgc gagcagcatg aacatcgggt
     1861 cgaagccgaa gccactcgac cggtacgcga tgcgcccgcc gcagccgccg ccgtagcagc
     1921 cgccgaagga gcagccgccg gtgacggttt cgagggtctc gagggtctcg atttcagagg
     1981 gagcttcggt ggtgcgttcg acgttcgaca tggtgggtcc tttggtggat gcagatcttc
     2041 tgatttgcag cgcccgtgcc tcaccgcgac cgcaatagtg acgggcacct gcgtcgctcg
     2101 gcccacgcgt gcgtcgtctc ccgtagtagg cggactgggg atggcctctg gccgaatata
     2161 tcgccttacg atataaagcc ggtcgtgcgt gaaatagagt tgtctgacta tcgagcgctt
     2221 gctgacttcc ggcaccagct gcgccgcttc atcgcgttct cggagaccgc agcccgcggg
     2281 gtgagtctcg aaccgcgcca gcatcaagta ctactcgcgc tacgcggctt gccggagggc
     2341 gtcgagccca cggtgcaagt gctcgcggat cgcctcgtgc ttcgccacaa cacggttgtc
     2401 gagctgctcg atcgcctgga ggccgaaggg ctgatccggc gggggcgcgc agcggaggat
     2461 cgccggcgcg ctgtcatcac gatcacggca cgcgggctcg agctcctgag caagctgtca
     2521 cggtcgcacc tggacgagct tcgctcgctg gcccccgcac tcgtcggagc actcaacagt
     2581 gcgctgcgtg catcgcggag ccgcgtatga aggagctagg tgatttcacc gcaacacgcc
     2641 ggatgattcc gatcaccggt gtcgcgatcg gcatcggcgt gctcgccgcg tttgtcgcgc
     2701 tggcattgct gcgtctgatc ggtctgttca cgaacctctg cttcttccag cgctgggaca
     2761 ccacgctcgt cgcgcccgca ggccatcacc tcggtccgtt cgtgatgttg gtgccggtcg
     2821 ccggcggcct catcatcggt ctgatggcga aatacggctc ggagaagatc cgaggccacg
     2881 gcattccgga ggcgctcgaa tcgatcttgc tccgcggcag ccgcgtcgag acgaagcttg
     2941 ccattctcaa acccctgtcg gctgcgatct cgatcggctc cggcggtccg ttcggtgccg
     3001 agggcccgat catcatgacg ggcggcgcgt tcggctcgct cgtcgcgcag cgcttccacc
     3061 tgacagcggc cgagcgtaag accctgctcg tcgccggttc cgcagcgggt atggcggcga
     3121 cgttcggtac gccgctcgcc gcgacgctgc tcgccgtcga gctcctgctg ttcgagtgga
     3181 agccgcgtag cttcgtcccg gtcgcgctcg cgtgtgcgag tgcaatggcg acgcgtcact
     3241 tcctgttcga cagcgcgccg ctattcccgg tgaatgcgcc ggtctccggc gacgacctcg
     3301 ccgtgctgct cggctgcatc ggcgctggcc tcggcgcggg cgtcttgtcg gcggcgatga
     3361 cggcgagcgt ctacttcttc gaggaccggt ttctgcgcct gcgcatgcac tggatgtggt
     3421 ggcccgcgct cggtggcctc gcgatcggca tcggcggcct catcgagccg cgcgcactcg
     3481 gcgtcggcta cgacgtgatc ggcgatctgc tgcacggtca tctcgcgctc gcggtgatcg
     3541 tgacactcgt gctgacgaag tgggcgatct gggcggtgtc gcttggctcg ggcacatccg
     3601 gcggtgtgct cgcgccgttg ctcatgctcg gtgcgtcgct cggcgcattg ctcgtaccga
     3661 tcatgccgag cgcgcccacg ggcttctggc agttgatcgg catgggcgcg atcctcggcg
     3721 gtacgatgcg ctcgccgctg acgggcatct tgttcgccgc ggaagtaagc ggtgacttca
     3781 atgcgctgct gccgctatcg gtcgcggtcg tcgtcgcgca tgcatttacg gtgctcacgc
     3841 tgcgccgctc ggtgctcacc gagaaggtcg cgcggcgcgg ctttcacatc acgcgcgagt
     3901 acgagatcga tccgctcgag gtgctattcg tgcgcgaagt gatgcgcccc gaggtgaccg
     3961 ccgacacgaa gggcgaagtc gcgttcgtcc aggaaacgct gcgcacggcg acactgcgga
     4021 tggcgaagca tggcgtgatc gcgctgcgcg tcgtcaacga gcagagccag ccggtcggca
     4081 cgatcgttct cgaggacgtg ctcggcgcgc gcaagcgcca cctcgaggaa gagacgcgcc
     4141 gcgaagcgct ccgcccgttg ccgtcatgga tgccgatccc gcagtgggtg ccgctggtgc
     4201 cgccgcgcga gcgctcgcga tcacttgccg tcgaggaacg cagccggcac gacgagtgac
     4261 gccgtctgct tcgcggcacc ggtcagcttg aactgcgagc acttgaactt cgcggtgctc
     4321 ggatcgcgcg gatcgcccag caccgcacac gccgtgtgca aggcagcctt catgccttcg
     4381 atcgtcgccg cgccgtcgac gacggtgcgc atgtagatcg gcatcacttg ctcggagaac
     4441 ggaaaccgcg tgccgtcgcg gagctcggcg agcgtctgcg gattggcggc cgcttggaac
     4501 gtgatcagct gggctaccgg cactggcgcg cccttgtcgg tcttgaccga aagcgtgagc
     4561 gtgatcgtgc ctggcgtcgc gtcgagctcg accttcgtct tcgcgccagc agtgacctcg
     4621 acccgacgga agtacatgtc cttcggtcga ttaccgccgc cgccgagcat cggagagatg
     4681 aggtacgagc caggcgcgag cgcgtcgagc gcgaacgtgc cgtccggacc cgtcgtgacg
     4741 aagaagttcg acgccacggc gccgatcggg ttcgcgatca cgacggtgtc gggcagcggc
     4801 tgacccgcgc gcacgacctt gccctcgaga ctgctggttc cggagaggac gaggtcgacc
     4861 gtcgcgctgt ccggcccggc gggaatctga atgctcgcgc tgcggccgac agctgacttg
     4921 ccggcgacca cggtgatcgg cccgggtgtg aagccttgca tcacgaaccg tccgttctcg
     4981 tcggtctcgg tgtcctgcgc catgatgctc tcgtccttga tgtagagctc ggcgccaccg
     5041 ccggtgagca gcatgcctgc agcgacggtt gctttcgcga ccggcgcacc gtgctcgtcg
     5101 atcacgcggc ccgacagcga gcggccggcg gccactgtga tcgtgccgag gtcggcgtcc
     5161 ttgccctcgg tgatcgtcgc ttgcttcgtc gtggtgacga agccggggcc actgatcgtg
     5221 agcggatacg tgccggcggc agcaggaagc gcaaacgcgc cgtccttcgc cgcgaacggc
     5281 agcggatttg tgccgccaag cgccgcggtg aacgcgatcg gtgccttgcc gtcggcgagc
     5341 tggaccttgc cgatcacgcg gccgtcggcg gggacgacga tcttgatcgc ggcgccgtta
     5401 ggcttggcag cgacgccttg cgacagctcg agcgcagctt cgctcgcacc gggacgcgca
     5461 gcgcgcaccc gatacgagcc gtcggggagg cccgcgaagc ggaatgcgcc gctctgatcg
     5521 gagacggtct cctgaacgcc gcgcacaacc catactgcac ggtcggcggt accgccggta
     5581 aattcgggct ctgcgatcac ctgcgcgtcg gcgatcggtt gcccggcttt gtcgacgacg
     5641 gtgccagcga tcgcgccgct gacgtcgagc ttgagcgcga cgtcttgctc gcgcttggcc
     5701 gcgagatcga cggcgacgat cgccgatgca ccactctcgt gccacgcgac gacgtccatc
     5761 ggtcgccggg cgagcccgct gatcgcgaac ttgccgtcgg cgtccgtgaa tgcctggcgg
     5821 cgcgctcgcc acggcacgaa gccgtgcacg acgacgctga catcagcggc cgccaccggt
     5881 ttgccggagc tgtcttgcac gacgccgcgc acgatcgcgc ccgacgtgag ctggatctcg
     5941 acgccactct tcgcgtgctc gccgtcgacg gtgagcggca tcgtggttgc gggcgcaaag
     6001 tcaccggcgg tggcggtgat gcgccaagtg cccgccgaga gcgcgggcag cgagaatgcg
     6061 ccatcggcgc cggtgagcac gccatcgcgc ctgggatcga cgaccggcaa cggctcggag
     6121 gcgaatgtcg gaacgacgcg cgcgttcgcg atcggtttgc ctttctcgtc gatcacacgg
     6181 ccggagagcg cggcgccatg tgcgagcgat agcgcgacgt gctccgtcgt gtcgccgtta
     6241 cccgacgtgc cgagcatcat cgcggcttgc gagtagccct tcgcccgtgc gacgagcggt
     6301 gcccagcccg caccgacgcc gcgcagctgt gcgatgccat cggcgttcgt cgtcgctttc
     6361 cacgtcagcg tggaacgtag ttcgacctca gcgcccttca cgggcgtgcc accttgtcgc
     6421 tcggtaattg cgacctcgac ggtgccgcct ttgtgcatgc gcaacgtgac tggttcgggc
     6481 ttgtcggcga gccgcagccg cgccgggccc gcgtaacgat cacccgccgt cgcttcgatc
     6541 cggtaatcgc gccggatcag gccgtcgaac acgaagccac cgtcggactc ggtctcgacg
     6601 accatcggtg ggttcgcatc gatcgcgacc atcgcatgag cgaccggcgc gtcgtgctcg
     6661 tcgatcacct ggccctccag gcgcagcgtg cctttcggat cgtcgtcgtg gatcgtcggc
     6721 agctcgcgtg tcgatccgtt ggtgggtgcg ggctcggtcg gcttcggcag cggtttcgtc
     6781 ggaggcaccg tcttcgcggt gaccgcgggc ttgtcttttt caccaccgcc ccacaggccg
     6841 gcgaagcgcg tgccggcgac gagcacggca gcggcgagag caacgagcaa taccttgata
     6901 cttgtcttca cgagcaatcc tcctgcgaac acggcggtgc catgaggcat cgcggctgga
     6961 agtggtgcga gcagaccggc ccatgccgtg cggtcattgc cgtgctcttc gtcgagcgcg
     7021 gcgcggatcc gatcgagcgc atccttgatc cgcgaccgaa cggtggccgc gggcacgcct
     7081 tgcgctcgtg cgatctccga cgcgttcatg ccctcgaaga agcgcagcag tagcgtcgag
     7141 cgcagcggct cgtgcaactc gaggacaagc cgaccgactt tctgttgcat ctgcacgcgc
     7201 gcgacgagct cttcgggcga tggcacctcg tcgtgcaccg gcgccgcttg ctcgcgctgt
     7261 tcgcgacgca cgcgaccccg cgtcgccatg cgcgccacgt tgcgcgcgac cccgccgagc
     7321 catggccgca gcggacctgc gcgcttcggc ggcttcgcga gcgcgacctc gtaggtgtcc
     7381 tgcaccacgt cgtcggcact cgcatcgccg acgagcgcac gcgcgacgcg cgacaaccac
     7441 tcggtatgcg cgagtagctc gtcagctgtg ggggcggatc ccgccatgcc cccatcttgc
     7501 cgccgccggg caacgtgttg ccgggcgacg gacgatttct acgtcatgca agtacgcgga
     7561 catagccgcg cgtgcaacgg gcaccgcgga tttgcgcgtc gtcgcgcacg aacccgttcg
     7621 cctcgtagaa cgcgatcgcg cgcgtcatct gctcgaccgt gtcgagcacc atatgcgtgc
     7681 cgcctcgttt gcgcgaccac gcgaccgcga cctcgagcag ctgcttacca aggccgagcc
     7741 cccgcgatgc gccacgcagg tacatcttgc gcagctcgta gatcccgggc gcgaccggaa
     7801 acacgccgca cgtaccgagt agctcgccgt ctcgcagcgc gatccagaac gcaccgccgc
     7861 gcgtggtgta ggaagcgggc aggccgtgca gctcggcgtc ggtggctgac cccttgccga
     7921 actcgagccc gaattcgccg agcacatcgg cgaccagacc gaggaccgcg gccacgtccg
     7981 tgtgttcgac gctgcggacc tcgatcattt gtcttccggc agaccttgtg cgcgccacgc
     8041 gcggatgccg ccatccatcg atgcgacgtt cgtgtagccc atcttctgca acgcttcggc
     8101 cgcgagcgcg gagcgaaacc cgccgccgca atagaggatg atctcgtcgc cgtgattggg
     8161 aattttggtc tcgatgtcgc gctcgatcac acctttgccg aggtggatcg caccggggat
     8221 atggccggcg cgccactcgt cgtcctcgcg cgtgtccacg aagtggaact tgtccccacg
     8281 tttcagccgt tcggcaacgg ccgatacggt ggtttctcgt atgttgcgct tcgcgtcgtc
     8341 gacgagcttc aagaaaccgg gcgagtgctt catggcgtcg gaagcctatc tcgatgggcc
     8401 cgccgtgcaa taccatcggg gcgtggaggt gattccggtc cacattgcgg tcctgactat
     8461 cgcggatgat gccgcgtcag ctgatcgtgc aaccgcccag gccatctccg agcgggcgac
     8521 cgccgcgggg caccagatcg tcgacgaaga agtggtcgcc gacacggaat cagcgattcg
     8581 cgatcagctc gtgcgctgga tcgcggagga caacatcgac gtggtcgtcg tcgcggcgag
     8641 tgtcgagagt gaggccgcat cggccgcgct ggcgcctctg gtccaccaga cgctgcccgg
     8701 gttcacggac ctgttccgct ggctgacatt ccaggaaatc ggtgcgtcgg cgatgctcag
     8761 cgcagccgaa gcggcgcagt gccagtcgac gttcgtgttc gtgctgcccg cacatgaagg
     8821 tgcggtgcgc gcggcgatgg acaagctgat cctgccgcag ctcgacgtgc gcacgaagcc
     8881 gaagaatctg gtcagccaga tgccgcggct caaggacgcc gcgaagaagt tcgcagatgc
     8941 gaacaagtcg caacgcgtgt cggttccggc ggccgcggtt gcgccgactc cgtccgcgcc
     9001 tgcaccggtc gcaattccgg cggccgccta cgcgccgcat ccaaccgcac gaccggaaac
     9061 cgagccggaa gcggtgccga agccgatcgc gcccgagaag acagaaggcg ggcacgggct
     9121 cccgccgaag ttgcccgccg gggtggcacc ggcggtggcg cgtagcaagc cgcgtacggc
     9181 gaacacgatc gcgcgcaaga ccgatgatcc accgacgaag gagatcgatc tcgcgaacct
     9241 cgagaagcag atccagctca gcaatcagga tgggccgacg aagaagatcg atctcagcgc
     9301 gaagacgaag gtcgtcgaca tgtcggcgca ccaggcgaag acgcgcgtcg tcgacagcgg
     9361 gcgcttgccg cgcgtgcctc ccggt
//
LOCUS       DASUFX010000082         9626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1263067, whole genome shotgun sequence.
ACCESSION   DASUFX010000082 DASUFX010000000
VERSION     DASUFX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263067"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..2431)
                     /locus_tag="VFV99_03175"
     CDS             complement(83..2431)
                     /locus_tag="VFV99_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058330.1"
                     /translation="MRKTLAGFLLASALAGCGIRHNYIKVDNVALGRVVVYRNGVAFY
                     ERRATVTGGALTVSVPRDRVDDFLKSLTVVDAISHKPLPVSIPRQQADDGSYLVMKLQ
                     LPTKDKADVVLTYVTESPSWKPSYRVVVGDKGKVMLEGWAIVDNTSGEDWKGVRVGVG
                     SSSALSFKYDLWSVRQVQRETLAAEEKFAIAPPTGISPYGGTRPEDIAKNGGEAVLTE
                     LGDEEIRRPDGHPESTTSRVAVKSEPAPTQPGMGAAVTGSVVQPSPPPPSAPMADMDD
                     GGGYEAESLSVEGGGGGGGGRYHVAKKPMRKVKTKDARKSTPTVQKAPTPQLNEQRVA
                     TGDQKMRVVSQQLVNSDKIIVIEGNADANRVGGNSRANDRANIVRNQLIDQGVAPGRI
                     KVITNVTPGNAERVRLIAQAPSPSEQAQAAKQKSVEMDAQPVGESHFANPTPMTVERG
                     ASAMVSMVRQETEGEVVYLYTPESERGNSNFAFRAVRFKNPTTSTLETGPVTVYGEEK
                     FIGEGLTEPIPPKSSAVVPYALDRQIVVERNTGEENKLQKLVTLERGVLTAEVQHIRR
                     QKMTITNRLNDVAKVFIRHTVNKGWKLLEAPPAFERVGEAHLFEVHLKPGETKTVEIA
                     EATPIERTLDLNADVTLDMMKVYVDAPEGSAELKDSMKKLLGIHRKLVDLMTEQTSLD
                     RRLRDYRERMDELHAQIVTLQAVKTGGDLMSHLKSKMKDISERVQKATIGMVDTEEKI
                     MLTRVEFQDALAELTLPDVTQTAGAAPVGAAIAAPPAVKLKK"
     gene            complement(2493..3065)
                     /locus_tag="VFV99_03180"
     CDS             complement(2493..3065)
                     /locus_tag="VFV99_03180"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5058331.1"
                     /translation="MMRAAMIPASMLEERRRLGHDRFDEVWEGVLHMVPPPPTVHKRV
                     ESNLQDVLKRIVAPRGFEVFHDPGLYDPAVPGQASYRVPDLAVVASEHLTMRGIEGLA
                     ELVVEIISPNDESRDKLPFYARVGVREVWLLDPARKSIEVFNGMTAVVSDARGRITAP
                     SLRLVLAIEGTTLKIKDGAEMHAVDIRDSL"
     gene            complement(3120..4142)
                     /locus_tag="VFV99_03185"
     CDS             complement(3120..4142)
                     /locus_tag="VFV99_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5058332.1"
                     /translation="MPELPDVTVYVERLAALTVGKPIDGIRIASPFVLRTVSPSPREL
                     YGAKVTGIERLGKRIVIGVEGDRYIVIHLMIAGRLRWKKRGEKIPNKVGLAAFDFPNG
                     TVSFTEASPKKRASIHIVAGRAALAEFDRGGLDVLGSTAAVFGERLRSERHTIKRSLT
                     DPTLFDGIGNSYSDEILHAAKLSPFRMTSSITDDEVKTLHRACIDVLTRYTKLLRDEV
                     GDGFPDKVTAFRPEMAVHGKYGQPCPVCGTKVQRIVYADNEANYCPTCQTEGRLLADR
                     SLSRLLKDDWPKTLEELEERKRPQSAAAVVAAPPSARPAIASVPETTAAERRPKRTAK
                     RDSSSG"
     gene            complement(4212..5069)
                     /locus_tag="VFV99_03190"
     CDS             complement(4212..5069)
                     /locus_tag="VFV99_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5058333.1"
                     /translation="MPARSIGTATISFGLVSIPTKLYTTNETSGDIHFNMLHEADGAR
                     LKQQYICTKCNEVVDREHTVKGYEHAKGQYVVFTAEELKALDAVATQTIALEEFVPAT
                     AVDPLYVEKSYYLGPDKGGERAYKLIRDAMLETELVGIASYSARGKQYIVCLRPFKEG
                     LILHQLRYSQEVKDWSEVPLPALPELKPAEIGLAKQIIQQIAHETFSADKYKDEVQER
                     MMQLINQKVEGQEITIAPEAPAGKVIDLMEALKASLGMSKTDDRKGAVAAEAKEEEPA
                     KKKTASKKK"
     gene            5093..7672
                     /gene="ligD"
                     /locus_tag="VFV99_03195"
     CDS             5093..7672
                     /gene="ligD"
                     /locus_tag="VFV99_03195"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5058334.1"
                     /translation="MSDPKDEGLDAYRAKREGGSTPEPMASTTGLSGKGPRMFVVHQH
                     DATRMHWDLRLEIDGVLCSWAVPKEPSLDPDDKRLAVKVENHPLEYVHFEAVIPAGNY
                     GAGPMICWDRGLFRPLIDPAQGMLDGEIKFELYGYKLTGAFTLVHTGKGTRGKFSGRG
                     SNDWLLIKKRDESAEAFLARGKPLSGASILSGLTIDELDARAPRHQAVVQALADAKVP
                     KKAIAHGSIEPMLCQTADAAFSSKDWIYELKWDGFRMMAYGGAGQAKLRYRSGLDPTD
                     RYPEITSAIRALPIPDLILDGEIVALDAEGKPDFHKLSFRGQMHRTSEIARAALSAPV
                     TYMVFDILGAAGHDLRGVPLIERKALLDKILPRLGPLRVSEHIPEHGEALLEQVVARG
                     MEGVVAKKANSTYKSKRSPDWLKLKKDPEADFAVCGYTPPKGTRTGLGALHLCVRVDS
                     QWVWAGKVGSGFDDKLLAQLTAELSAKPRWKPTFPRPEGSGDALWIEPELVVQVRYRE
                     WPEDSSLRFPVFERLRRDKNPMDCTMPARRTGQAAEPREPKPGESMELVVDSQVRELR
                     LSNPKKVFWKDENITKGDLIEYYRSVAPWLLPYLKDRPTVLHRYPDGIDGDMFYQKDM
                     PDWVPPWLRTTTLWSEHSQREIHYVLIDDPDGLAFVANLASIPIHCWASRIADLERPD
                     WTIIDLDPKNAPREHVVPLALAIHRLCDDIGLPNFVKTSGQSGLHVLIPLGGQCTFDQ
                     ARTLAYLIGLIIERQHPDMATTNRNPQARGGRVYLDWGQNAHGQLLVAPYSVRPVANA
                     PVSMPLVWDEVVPELDAKQFNLRNALDRLTSWPGDPIAPVLEMRPDLAAALTALEKYA
                     KDA"
     gene            complement(7669..8844)
                     /locus_tag="VFV99_03200"
     CDS             complement(7669..8844)
                     /locus_tag="VFV99_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058335.1"
                     /translation="MRVTRIGLVVGFALAACGTDSINDGADLSGAATKHPPDHKPPVG
                     ATGQEIFRFDTFGDEQLWTDKLRMHEVIASAVSPTIALSVGLKVDADALPPGILATAD
                     LNDPKTTVALIGLDAVVGVMGKVDGSGKLHEVGITCALCHSTVDDSVAPGIGHRLDGW
                     ANRDLDPGAIIALSPALTDAQRAVYSSWGPGYYDARYNIDGINDPAVIPPAYGLAGVA
                     AETYTGDGPVSYWNNYVAVTQMGGHGNFSDPRIGINIVQTPDLVTPKLPALRDYQLSI
                     PKPPIPESFNVASAARGKAVFEANCSRCHEGAIYTDVARTLHAAAETGMDPLYATRSA
                     TKLYRTTPLRALWQHPPYFHDGSAKTLEDVVDHYARVLGLRLADCDKSDLVAFLKTI"
     gene            complement(8926..9570)
                     /locus_tag="VFV99_03205"
     CDS             complement(8926..9570)
                     /locus_tag="VFV99_03205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5058336.1"
                     /translation="MLRSTRAIVFDLDGTLVDSLPDIMTHLNGALVDHGLPSYNREEI
                     GHWVGYGAEQLVIRAVPQQDLVAPVLATFRARYRSRPVIATRVFDGLGEVLDGLAPRF
                     SLAVLSNKPHDLTAHVCTALLGRWPFKVVAGARPDRPHKPDPQALRDVVADLGVTVET
                     TVLVGDSEVDVATAHAAGATSIAVAWGLRPVAVLEAANPTHLLHAPHELAALFA"
BASE COUNT         1599 a         3172 c         3099 g         1756 t
ORIGIN      
        1 atcaccgcac gcgctgcacg ctgcgaggag cggaaggaag ccgagtcgct tcatgcgcga
       61 acgctagcac gcgtgcgtgc ggttacttct tcagcttgac ggcgggaggc gcggcaatcg
      121 ctgcgccgac cggtgcagct cctgcggtct gggtcacatc cggaagcgtg agctcggcga
      181 gcgcgtcctg gaactccaca cgcgtcaaca tgatcttctc ttcggtgtcg accatgccga
      241 tcgtcgcttt ttgcacgcgc tcggagatgt ccttcatctt cgacttgagg tgcgacatca
      301 agtcgccccc cgtcttcacc gcctgaagcg tgacgatctg cgcatgcagc tcgtccatgc
      361 gctcgcggta gtcgcgcagc cggcgatcga gactcgtctg ctcggtcatc agatcgacca
      421 gtttgcgatg gatgccgagc agcttcttca tgctgtcctt gagctccgcc gagccttcgg
      481 gcgcgtcgac gtagaccttc atcatgtcga gcgtgacgtc ggcgttgagg tcgagcgtgc
      541 gctcgatcgg cgtcgcttcc gcgatctcga cggtcttcgt ctcgcccggc ttgaggtgca
      601 cctcgaacag gtgcgcttca ccgacgcgct cgaacgcggg cggcgcctcg agcagcttcc
      661 agcccttgtt cacggtgtga cggatgaaca ccttcgcgac gtcgttgagg cggttcgtga
      721 tcgtcatctt ctggcgccgg atgtgctgga cctcggcggt gagcacgccg cgctcgagcg
      781 tgacgagctt ctggagcttg ttctcctcac cggtgttgcg ctcgacgacg atctgacgat
      841 cgagcgcgta cggcacgacc gccgatgact tcggcggaat cggctcggta aggccctcgc
      901 cgatgaactt ctcttcgccg tagacggtga ccggtccggt ctcgagcgtc gaggtcgtcg
      961 ggttcttgaa ccgcaccgcg cggaacgcga agttcgagtt accgcgctcg ctctccggcg
     1021 tgtacagata cacgacctcg ccttcggtct cctggcgcac catcgacacc atcgcgctcg
     1081 caccgcgctc gaccgtcatc ggcgtcgggt tcgcgaagtg actctcgccg accggctgcg
     1141 catccatttc gacgctcttc tgcttcgcgg cctgcgcctg ctcgctcgga ctcggcgcct
     1201 gcgcgatgag ccgcacgcgc tccgcatttc ctggcgtcac gttcgtgatc accttgatgc
     1261 ggccgggcgc gacgccctga tcgatgagct gattgcgcac gatgttcgcg cggtcgttcg
     1321 cgcggctgtt accgccgacg cgattcgcat ccgcgttgcc ctcgatcacg atgatcttgt
     1381 ccgagttgac gagctgctgc gacacgacgc gcatcttctg gtcgcccgtc gcgacgcgct
     1441 gctcgttcag ctgcggcgtc ggcgccttct gcacggtcgg cgtggatttg cgcgcgtcct
     1501 tcgtcttcac tttcctcatc ggcttcttcg cgacgtgata gcgaccgcca ccgccgccac
     1561 cgccgccttc gacgctcagc gactccgcct cgtagccacc gccgtcgtcc atgtccgcca
     1621 tcggcgccga gggtggcggc ggcgacggtt gcaccacgct accagtgaca gcggcgccca
     1681 tgcccggctg cgtcggcgcg ggctcgctct tcaccgcgac gcgcgaggtc gtgctctccg
     1741 ggtgaccgtc gggacggcga atctcctcgt cgccgagctc ggtcagcacc gcttcgccgc
     1801 cgttcttcgc gatgtcctcg ggacgcgtgc caccgtaggg cgagatgccg gtgggcggcg
     1861 cgatcgcaaa cttctcctct gcggcgagcg tctcgcgctg cacctgacgc acgctccaca
     1921 ggtcgtactt gaatgacagc gcagacgagg aaccgacgcc cacccgcacg cccttccagt
     1981 cctcgccgct cgtgttgtcg acgatcgccc agccctcgag catcaccttg cccttgtcac
     2041 cgacaacgac gcggtagctc ggcttccacg acggcgactc ggtcacgtat gtcagcacga
     2101 catcggcctt gtctttcgtc ggcagctgca gcttcatcac gaggtagctg ccgtcatcgg
     2161 cttgctgtct cggaatcgag accggcagcg gcttgtgcga gatcgcatcg acgacggtca
     2221 gcgacttcaa gaagtcgtcg acgcgatcgc gcggcacgct gaccgtgagc gcaccgccgg
     2281 tcactgtcgc acggcgctcg tagaacgcga cgccgttgcg gtagacgacg acgcggccga
     2341 gcgcgacgtt gtcgaccttg atgtagttgt gcctgatgcc acaccccgcg agggcggagg
     2401 cgagcagaaa tccggccagc gtcttcctca tgttctcggc gcaaacgcac cgagtcgaag
     2461 cgcggtctaa aaaacttccc aagaagcagc ggtcataggg agtcgcgaat atccacggcg
     2521 tgcatctcgg cgccgtcctt aatcttcagc gtcgtgccct caatcgccag cacgagacga
     2581 agtgacggcg cggtgatgcg gccacgggcg tcgctgacca ccgcggtcat gccgttgaac
     2641 acctcgatcg acttcctagc gggatcgagg agccacactt ctcgcacgcc aacccgcgcg
     2701 tagaacggca gcttgtcgcg actctcgtcg ttaggcgaaa tgatctcgac aacgagctct
     2761 gcgaggcctt cgattcctcg catcgtcaga tgctccgagg cgacgacagc aaggtcgggg
     2821 acccggtagc tggcttgccc aggtactgcc ggatcataca gtccgggatc gtggaacacc
     2881 tcgaaaccgc gaggtgccac gatgcgtttc agcacgtcct gaagattgga ttcgacgcgc
     2941 ttatgcacgg tcggcggcgg cgggaccatg tggaggactc cctcccagac ctcgtcgaac
     3001 cgatcgtggc cgaggcgacg acgttcctcg agcatcgagg caggtatcat cgctgcgcgc
     3061 atcatgcgtc gactatagcc cagggacatc cgccgccctt cagcaacgca tatgccgcgc
     3121 taaccgcttg aactgtcgcg cttggctgtc cgctttggcc ggcgctcggc cgcggtcgtc
     3181 tccggtacgg acgcgatcgc cggacgcgcg ctcggcggtg cagcgacgac cgcagcagct
     3241 gactgcggac gcttgcgctc ctcgagctcc tcgagcgtct ttggccagtc gtccttgagc
     3301 aggcgcgaca gcgatcgatc ggcgagcaac cgcccttcgg tctgacacgt cgggcagtag
     3361 ttcgcctcgt tgtccgcgta gacgatgcgc tggactttgg tgccgcacac cggacacggt
     3421 tggccgtact tgccgtgcac ggccatctcg ggccggaacg cggtgacctt gtcggggaag
     3481 ccgtcgccga cctcgtcgcg cagcagcttc gtgtaccgcg tgagcacgtc gatgcaggcg
     3541 cggtgcaacg tcttcacttc gtcatcggtg atcgacgacg tcatccgaaa cggcgacagc
     3601 ttcgccgcgt gcaggatctc gtcggagtac gagttgccga tgccgtcgaa cagcgtcgga
     3661 tcggtgagcg agcgcttgat cgtatggcgc tcgctgcgca gccgctcacc gaacacggcg
     3721 gcggtcgagc cgagcacgtc gagcccgccg cgatcgaact cggcgagcgc cgcgcggccg
     3781 gcgacaatgt ggatcgacgc gcgcttcttc ggcgacgcct cggtgaagct caccgtgccg
     3841 ttcggaaagt cgaacgccgc gagcccgacc ttgttgggaa tcttctcgcc gcgcttcttc
     3901 cagcgcaggc gccccgcgat catcagatga atcacgatgt agcggtcgcc ctccacgccg
     3961 atcacgatcc gcttgccgag gcgctcgatg ccggtgacct tcgcgccgta cagctcgcgc
     4021 ggcgatggcg acaccgtgcg cagcacgaac gggctcgcga tgcggatgcc gtcgatgggc
     4081 ttgccaacgg tcagcgcagc aagccgctcg acgtagacgg tgacgtcagg cagctcgggc
     4141 acggccagac catagcaggc ctctggcctc agacctcagg cctcaggttc agagcgaagg
     4201 cctgacctgc gctacttctt cttcgaggcg gtcttcttct tcgccggctc ttcttccttc
     4261 gcctcggcgg cgaccgcgcc tttgcggtcg tcggtcttcg acatgccgag gctcgccttc
     4321 agcgcctcca tcagatcgat gaccttaccg gccggcgcct cgggcgcgat cgtaatttcc
     4381 tggccttcga ccttctggtt gatgagctgc atcatccgct cttggacttc gtccttgtac
     4441 ttgtcggcgg agaacgtctc gtgcgcgatc tgctggatga tctgcttcgc gagcccgatc
     4501 tcggcgggct tgagctcggg gagcgcgggc aatggaacct cgctccagtc cttcacctct
     4561 tgcgagtagc gcagctggtg caggatcagg ccctctttga acgggcgcag acagacgatg
     4621 tactgcttgc cgcgcgccga gtacgacgcg atgccgacca gctcggtctc gagcatcgcg
     4681 tcgcggatca gcttgtacgc gcgctcgccg cccttgtcgg gaccgaggta gtagctcttc
     4741 tcgacgtaga gcgggtcgac agcggtggcc ggtacgaatt cctctagcgc gatggtctgg
     4801 gtggcgactg cgtcgagcgc cttgagctct tccgcagtaa acacgacgta ttgccccttg
     4861 gcgtgctcgt agcctttgac ggtgtgctcg cggtcgacga cctcgttgca cttcgtgcag
     4921 atgtactgct gcttcaaacg cgccccatcg gcctcgtgaa gcatgttgaa atggatgtct
     4981 cccgaggtct cgttcgtcgt gtagagcttg gtcgggatag aaaccaggcc gaacgaaata
     5041 gtggccgtac cgatggaacg agcgggcatg aagtaaattg taccgggctg tcgtgagtga
     5101 tcccaaagac gaaggtttgg atgcttaccg tgccaagcgc gagggtggga gcacacctga
     5161 accaatggcc agcaccactg gcctatcagg caagggtcca cgcatgtttg tcgtgcatca
     5221 gcacgacgcg acccggatgc actgggatct gcgcctcgag atagacggtg tgttgtgctc
     5281 gtgggccgtt cccaaggagc cctcgctcga tcccgatgac aagcgactcg cagtcaaggt
     5341 cgagaaccac ccgctcgagt acgtccactt cgaagcggtg attccggccg gcaactacgg
     5401 cgccggtccg atgatctgct gggaccgcgg cctgttccgt ccgctgatcg atccggcgca
     5461 gggcatgctc gatggcgaga tcaagttcga gctctacggc tacaagctca ccggcgcgtt
     5521 cacgctcgtg cacaccggca aggggacgcg cggcaagttc tccggccgcg gcagcaacga
     5581 ctggctcctg atcaagaaac gcgatgagtc cgcggaagcg ttcctcgcgc gtggcaagcc
     5641 gctgtcaggc gcatcgatcc tgagtggtct cacgatcgac gagctcgatg cgcgcgcgcc
     5701 tcggcatcaa gcggtcgtgc aggcgctcgc cgacgcgaag gttccgaaga aagcaatcgc
     5761 gcacggctcg atcgagccga tgctgtgtca gaccgcggac gctgcgttct cgtcgaagga
     5821 ctggatctac gagctcaagt gggacggctt tcgcatgatg gcgtacggcg gcgcgggaca
     5881 agcgaagctg cgctatcgct ccggtctcga tccgacggat cgctatccgg agatcacgag
     5941 cgcgatccgc gcgctgccga ttcccgacct gatcctcgac ggtgaaatcg tcgcgctcga
     6001 tgccgaaggc aagcccgact ttcacaagct gtcgttccgc ggccagatgc atcgcacgag
     6061 cgagattgca cgcgccgcgc tctctgcacc ggtcacgtac atggtgttcg acatcctcgg
     6121 cgccgccggc cacgatctgc gcggggtgcc gctgatcgag cgcaaagcgc tgctcgacaa
     6181 gatcctgccg aggctcgggc cactccgcgt gtcggagcac atccccgagc acggcgaggc
     6241 actgctcgag caagtcgtcg cacgcggcat ggaaggcgtc gtcgcgaaga aggcgaattc
     6301 gacgtacaag agcaagcgct cgccggactg gctcaagctg aagaaggatc cggaggccga
     6361 cttcgcggtg tgcggctaca cgccgccgaa gggcacgcgc accggcctcg gcgcgctcca
     6421 tctctgcgtg cgcgtcgaca gccagtgggt ctgggccggc aaggtcggct ccggcttcga
     6481 cgacaagctg ctcgcgcagc tgacggccga gctgtccgcg aagccgcgct ggaaaccgac
     6541 gttcccgcgt cccgaaggca gtggcgacgc gctgtggatc gagcccgagc tggtcgtgca
     6601 ggtgcgctat cgcgaatggc ccgaggactc gtcgttgcgc ttcccggtat tcgagcgatt
     6661 gcgccgcgac aagaacccga tggattgcac gatgccggcg cgccgcaccg gccaagctgc
     6721 agagccgcgc gagccgaagc ccggcgagtc gatggagctc gttgtcgatt cgcaggtgcg
     6781 cgagctacgg ctgtcgaatc cgaagaaggt gttctggaag gacgagaaca tcacgaaggg
     6841 cgacctcatc gagtactacc gctcggtcgc accgtggctc ctgccctacc tcaaggatcg
     6901 gccgacggtg ctgcatcgct accccgatgg catcgacggc gacatgttct accaaaagga
     6961 catgcccgac tgggtgccgc cgtggctgcg caccacgaca ctgtggagcg agcacagcca
     7021 gcgcgagatc cactacgtgc tgatcgacga ccccgatggg ctcgcattcg tcgcgaacct
     7081 cgcgtcgatt ccgatccact gctgggcgag ccgcatcgcg gacctcgagc gtcccgactg
     7141 gacgatcatc gatctcgatc cgaagaacgc gccgcgcgag catgtcgtgc cgctcgcgct
     7201 cgcaatccat cgcttgtgcg acgacatcgg cctgccgaac ttcgtgaaga catcgggcca
     7261 gagcggtctg cacgtgctga ttccgctcgg tggccagtgc acgttcgatc aagcgcgcac
     7321 actcgcgtat ctgatcggcc tcatcatcga gcgccagcat cccgacatgg cgacgacaaa
     7381 tcgcaacccg caggcacgcg gcggccgcgt ctatctcgac tggggacaga acgcgcacgg
     7441 tcagctgctc gtcgcgccgt acagcgtgcg gcccgtcgcg aacgcaccag tgtcgatgcc
     7501 gctcgtctgg gatgaggtcg tgcccgagct cgatgcgaag cagttcaacc tgcgcaatgc
     7561 gctcgatcgg ctgacgagct ggccgggcga tccgatcgcg ccggtgctcg agatgcgtcc
     7621 cgatctcgcg gccgcgctga ccgcgctcga gaagtacgcg aaggacgcct agatcgtctt
     7681 caggaacgcg acgaggtcgc tcttgtcgca gtcggcgagg cgaagaccga gaacgcgagc
     7741 gtagtggtcg acgacgtcct cgagcgtctt agcggagccg tcgtggaagt acggtggatg
     7801 ctgccagagc gcacgcagcg gtgtcgtgcg gtacagcttc gtcgcgctgc gcgtcgcgta
     7861 gagtggatcc atgccggtct cggcggctgc atggagcgtc cgcgcgacgt cggtgtagat
     7921 cgcaccctcg tggcaacgcg agcagttcgc ctcgaacacg gccttgcccc gcgcggcgga
     7981 cgcgacattg aagctctccg gtatcggcgg cttcggaatg ctcaactggt agtcgcgcaa
     8041 tgcgggcagc ttcggggtca cgaggtccgg cgtctgcacg atgttgatgc cgatgcgcgg
     8101 atcggagaag ttgccgtggc cgcccatctg cgtgacggcg acgtagttgt tccagtacga
     8161 caccggacca tcgcccgtgt aggtttcggc ggcgacgccg gcgaggccgt acgcaggtgg
     8221 gatcaccgcc ggatcgttga tgccgtcgat gttgtagcgc gcgtcgtagt agccggggcc
     8281 ccacgacgaa tacacggcac gctgtgcatc ggtgagcgcg ggtgacagcg cgatgatcgc
     8341 gcccggatcg agatcgcgat tcgcccagcc gtcgagccga tggccgatgc ccggcgcgac
     8401 cgagtcgtcg actgtcgagt gacatagtgc gcacgtgatg ccgacttcgt gcagcttgcc
     8461 gctgccgtcg accttgccca tcacaccgac aaccgcatcg aggccgatca gcgcgaccgt
     8521 tgttttcgga tcgttcagat cggcggtcgc gaggatgccc ggcggcaacg cgtcggcatc
     8581 gaccttgagg ccaaccgaga gcgcgatcgt cggtgacacg gcggacgcga tcacctcgtg
     8641 catgcgcagc ttgtcggtcc acagctgttc gtcgccgaac gtgtcgaacc gaaatatctc
     8701 ctgacccgtc gccccaactg gaggcttgtg gtcgggcggg tgcttcgtcg ccgctccgct
     8761 gagatccgcg ccgtcattga tcgaatcggt tccacacgcc gcgagcgcga agccgactac
     8821 aagtccaatt cgagttactc gcatgcatgt agtgcgagca acgcgagtgc cacgcggagc
     8881 ggtccgatcc ggtgtcgtca gcgccctcgg gtgatcgctg aaccgctatg cgaacaacgc
     8941 cgcaagctcg tgtggcgcat ggaggagatg agtcgggttg gcagcctcga gcacggccac
     9001 cgggcgtagc ccccacgcca ccgcgatgct cgtcgcaccc gcagcgtgcg ccgtcgcgac
     9061 atcgacttcc gagtcaccga cgagaacggt cgtttcgact gtaacgccga ggtcggcaac
     9121 gacgtcgcgc agcgcctgtg gatcgggctt gtgcggccga tccggacgcg cccccgccac
     9181 gaccttgaac ggccagcgcc cgagcagcgc ggtgcagacg tgggccgtga gatcgtgcgg
     9241 cttgttcgac agcacggcga gcgagaagcg cggcgcgagc ccgtcgagta cctcgcccaa
     9301 accgtcgaag acgcgcgtcg cgatcaccgg gcgcgagcga tagcgcgcgc gaaacgtcgc
     9361 aagcaccggc gcgaccagat cttgctgcgg gaccgcacgt atcacgagct gctcggcgcc
     9421 gtaaccgacc cagtgcccga tctcctcgcg gttgtacgac ggcaagccgt gatcaacgag
     9481 cgctccgttg aggtgcgtca tgatgtccgg aagactgtcg acgagcgtgc cgtcgaggtc
     9541 gaagacgatc gcgcgcgtgc ttcgaagcat gttgcttacg gcttgccggt cctgagcatg
     9601 cgaatttccg ctctgagctt cgctat
//
LOCUS       DASUFX010000083        21466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1124434, whole genome shotgun sequence.
ACCESSION   DASUFX010000083 DASUFX010000000
VERSION     DASUFX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21466
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124434"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFV99_03210"
     CDS             complement(<1..536)
                     /locus_tag="VFV99_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058337.1"
                     /translation="MPDRKLPQLETGRLETIVLAVALVVLVLDAGGGPGWNVAAANAV
                     GAARLMHVASVPLYNVLASAAALLPWGEVGFRLAILDAVLAAFTLAGVVAAVRALVPR
                     DVGAALVSVALLLIAPPFRDAAAFASPAILAACGAVWSVAFAARYARDNDARNGAAAI
                     GACAVVVGSTPWLGAVIT"
     gene            578..1525
                     /locus_tag="VFV99_03215"
     CDS             578..1525
                     /locus_tag="VFV99_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058338.1"
                     /translation="MAQIRIVDDWLRSPASPPSHYRSVMVIAEKWAEQRVLMRAYAQS
                     RDQTMPTIMLHAVELAIGPAGLDVNGPWGIHVGSNPGMEAQAVKAGLEDAARRMAGSK
                     GNPPRLVDESNTFDREPTGNWAPGTPRVLPQRPDQRAPAVEHVAAAAAARVPQAQMAT
                     YIPQAPAAQHVPQAQTAVVAPSNPELRKTPVPRRSSGKTRPPPTNVRTALGYTSGSGA
                     QSAIVKLGLAPQISARLGRLVDRVVPADFSIDAGERRVLNALGEHALTARSIGHMLDI
                     ADATTFMEELTSKLERYGLGDLIEPGEPQGGEPTYRMRR"
     gene            1568..2944
                     /gene="tig"
                     /locus_tag="VFV99_03220"
     CDS             1568..2944
                     /gene="tig"
                     /locus_tag="VFV99_03220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5058339.1"
                     /translation="MQVRIEEVSPVEKKMFVEIPWETVSQKLGDAYRELSKGVALKGF
                     RKGKVPRPVLEQVYGPRVNAEVAYELVRESFFRANQEHNLAAVAEPRVEEADPIKKGQ
                     PFKFAAIIEVRGEVVPQDYKGLSIERRKLNIADSAVEESLAQLRREHTELRPIEGREV
                     TAAGDIVGLSITGTIGEHQINQPQFAVDLDDVEREPLPGIREALLGIPLDTKEKKLEL
                     TVPDDYKDESLRGRKADLTVTILEARAKDVPELDDEFAKDTGKADTLDGLKAALRKEL
                     EDRETEVIDREAREGVLRELIKKNQIPVASSLVDRAVEMQYQRLRQMLGMKPERNAQI
                     PDDLKDKMKPAGADEVRGQLLLEAIADKENVVVTDEELGKHVEQTAKARNMAPAKLRA
                     EWQRDGRLDSVTYSLRQDKVLKFLVDAAQITEVEKLTQPGTPLPEGPAHGEEGHVHGP
                     DCDHDHDH"
     gene            3050..4981
                     /locus_tag="VFV99_03225"
     CDS             3050..4981
                     /locus_tag="VFV99_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058340.1"
                     /translation="MESDKTQPGMSGPHPTIAQLRNPTEPGIDDHVASCSMCRSVMAA
                     DVDETPIPLELPKGLVSETAFKWPPDPIARGGMAAIFAGEDRRLGRTVILKTPREDEN
                     LPKYMTDMFQSRVTAEARVLAKLQHPSIVTIYELGKSSVGWPFCVLERVDGTSLRERL
                     DELADAEEKLMKPATRERLELLSNLVSIAEALAYAHERRVVHRDVTPNNILLGKRGEA
                     TLIDWGIARDLDAPGGTEPSIHDEDPSTTGRMVTISAGTPPYIPLEQSQGRKADPSFD
                     VYSFGVTLYEVVSGKTPFVWGGATSPGGRTAQVDAFMAWLQSDDDIPPAMPKDTELSG
                     IIAKAMARKPADRFTADELVRALKQYLTGDLVFSHRYSLSGRLARWARRHKTATVSML
                     FLTVLVVAGALGWAAWSRQQKEEAELRALAASAQVDASEKGRLADEASREADAAKALA
                     EQAQREGKDAQALKREADRKRKAAEETRRAAEAAATQAKGTAEDALKRFQQAMKEKDE
                     ANTQRDAANSARDQAEREREAARASQVAAERDRDASKAARDAADKERDAAKQTAAAAE
                     AERNAARAARDASDKERDAARAAQAASEKERDAAIAARSNIEKERDEARRRVADLEQK
                     LRELQQGGPPGPPPPPPSP"
     gene            5123..5716
                     /locus_tag="VFV99_03230"
     CDS             5123..5716
                     /locus_tag="VFV99_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058341.1"
                     /translation="MLGRLTLSLLMTLTISSVVACTDLTSEDEETSNLSSEEQACMHD
                     EAAKLAVLKKLHDRYQDINVALADGYKLGVIYNGNLVVNGCVSNLANPALGAMGYHYF
                     LQDRFDDAKIKELKPEVLVYHTGANGTRVLGAVEWVVPKPAWDAKHGVDADAPEVYGQ
                     MMMILNPMLNWYVAHAWLWKDNPSGVLSDWNPDVTCP"
     gene            complement(5770..6933)
                     /locus_tag="VFV99_03235"
     CDS             complement(5770..6933)
                     /locus_tag="VFV99_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058342.1"
                     /translation="MWRVGCCLVLGGCALAACGRIDFAELNANGGDAPGSGDGPNGSG
                     FSSLVAYGDQTCALYKGTAYCWGANNNGQIGDGSNNDAITPSRVLLPAGSVNALSEGD
                     VSGCAIVDANLYCWGTPTGPTPTQIPLGAPATAVAAGKNFSCVIADMTRCWGQNDTGQ
                     CGTGSVAPPATPTSVNFIPAYPATQIDAGDDHACAFAPGQPPQCWGHNDSGALGTGSM
                     SPTTSVDPVGVIGGITSLPRIAGWHACGLVGGTVWCWGEGNSGELGNGMTNNSATPVM
                     VSGLSNVTALETGGGPVDHDASCAISGGNVSCWGRGELGRLGNGMTVSESTPVDVLGL
                     PGAATSVAVGFAHGCALLVDGDIWCWGRGDRGQLGDGNKTDSMAPVKVVRPTT"
     gene            complement(6947..7609)
                     /gene="radC"
                     /locus_tag="VFV99_03240"
     CDS             complement(6947..7609)
                     /gene="radC"
                     /locus_tag="VFV99_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5058343.1"
                     /translation="MTLVDRPRERLWSRGTTMIGDEELLSIVLGTGVRDRPALAVASE
                     LVKATGSVAALSRASPRELALVNGVGTARATRIAAAFELGRRALESEQHRTILGHPAD
                     VFGIVGPRLAGLAQEVFIVMGIDIRNGLLDVVEVARGSVHAVEVHPREVFRPLVRMAA
                     AGAVLVHNHPTGDPTPSPEDIALTERLRSVGDVIGIPIIDHVVVGDGKYRSIAEWMGA
                     VL"
     gene            7791..9995
                     /locus_tag="VFV99_03245"
     CDS             7791..9995
                     /locus_tag="VFV99_03245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058344.1"
                     /translation="MFVCAECGASQPGPGPCRADGTPMMPIGDDLLLGTTIGAYRVAR
                     LLGVGGMGRVYKGVHPTIGSRVAIKVLSRECSDRRDLVARFFSEAKAVNVISHESIVN
                     VLDLATLPDGRPYIVMEYLDGSPLAGIIEHAQRSGMPVPLGGLARLVAEVLDALGAAH
                     AKGIVHRDLKPDNIYVTPAGRAKVLDFGIAKLMQPVGEAASGSATHTGSLLGTPHYMS
                     PEQAAGRPVDARADLYAIGVILFECSTLQKPFIADSLFDLLRKHVEAPPPSPRALRRD
                     MPEALEHIIYTALAKLPDQRFASAHAMSIALQHATMNLPSEQWTPIQPASSSRSAPSG
                     GWNPTPPASWAGGNRPSVAVAPTQTAGQVTRGERGGSKKGLWLALGALVLVGGGITAA
                     VIAGGGKGDKQVVAQGSGSEAAASGSDAWATPGSAARDGVTPPVTPPVEHEAPDPDDD
                     ADDGELDAASTEAMDQAFAEAFNNLDPAARAMFPPEVNAALKKYGKWSKIPKAERMKL
                     LAKIAGMTQNLTKSSRDINRALAGDPGSTAPTPPTTPPTTPKTSGGPVAGLDANGWVV
                     SRRFNPSGYNKKNLSVEKYLPTAIAEAKKLVPDAVLFRIDADGVYPDGHADITLAEHG
                     SLDFRFISQKRAKGDPKLPLGAKQEYKCMFRIMFDDEGGWSAPIDGWECKETLIGSPK
                     CTTAQVWKKAIAKGAPANAIAELGYRAWDGKARWYFDIGGTKFSEVFDDDCR"
     gene            complement(9992..12628)
                     /locus_tag="VFV99_03250"
     CDS             complement(9992..12628)
                     /locus_tag="VFV99_03250"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058345.1"
                     /translation="MGRRLVVFAILLVACGDNLKPEPDEDTIVQVKLETHAPAQVAAG
                     DPINVSCTLTENDIESMVTGEIAVTAETSVIRMGAEIIAKTAGSVDIACVLPGRGIVD
                     TTPATVEIVAGAAANLVTTIVPDPVVAGNSITTTCVVYDAYGNVVTGTMPTLQLTPDD
                     SANTITDLQALMTRAGHYTGRCYLPGTTSNNAAFDVVPNLPANLAISKFPDLPVYAVG
                     QTVQVDAVVSDRYGNDIAPPPVTNTSAPVTGVGPTMIVNANTFRYNGEGLYRITVAVT
                     PPTDNNVPLSATTDVVVNSRGPAVTCAGDATMVNLTPGSTMTVTGQANDVNGVSSISV
                     NGNSVAVGANGAFSAPIVTRFGLNFVDVTALDAFGEPTTKLCTYLVSNTYGSPTNLIN
                     DEISLKLTQAAVDDGNRGGAINSLADVLYVVLNSSGLATTIDSSLKASNPLKPQSCDS
                     QVCIPLIGCACLYSSRIDYQGLSLPGPQTVDLTLTTNGIIAHAHIPNIGVNLRVRGAV
                     SGIGYDTSGWVTVSYIDVTLTLDLSISGGKPHISVRPGSVSSAVGSISTAFGGVDGWI
                     INNIVVPLAQGSLRTTLTNLITNFVTNNFNAVLDGLVANLDISTLGTSFNVPRLDGSG
                     NVPLSFGVGFSSLSTTTSRALFGISTKFTAPAANAYPSLGVPLPTGTILLDPTVSAPS
                     NTGVGAHIGIIEHALHALWRANYLAATLTGAQLGAGLPAGTTISIVTRLPPVATILGN
                     GTVQLQLGAIDLVIQHPFLPVNLAVRAGADAHASVTLVGNDLVFGNIVIDQTHVGTDD
                     VNLDASEQQSLQTALNTLLQQLVNQSLNNALPAIPIPSFTIPASLGQYGLPVGGQLGI
                     KSPSLSVAPQHFTLRGQFGVLP"
     gene            complement(12674..14050)
                     /locus_tag="VFV99_03255"
     CDS             complement(12674..14050)
                     /locus_tag="VFV99_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5058346.1"
                     /translation="MKLVVTLGALFVSAPVFAQPQPTQPQPPQPQPAQPQPKPQFVQP
                     PAPTPPPPNAQTLTMAEAVALAMRQDPSLRQSRASIEVARGRVDQVQATRRPTLTVTG
                     NLTASGGGGSGALARDFLDPTTGASVGGAASWRIYDFGQTAAQLHAAKLNAEAVTAGL
                     DTTMLDVVRNVELSYLEAVARARLVTVAEATVKSEEAHVDQAKRFVAAQAKDPIEVAT
                     AQARAANARAALALAYSNQAIALATLRAAIGAVDPNQSFVVANSWPVPPGDAPPLTSL
                     VETARRQRPELVQADKEVAASDASIDAASAQLRPVLSAAARADWSPQFGGNWEHPGWT
                     AGLTLSWPIYDGGRTKADVRIARANKQSAIAQRDALLIDLTSQLDATRSQIEANRAGV
                     ESSKEAVDAANAQLKLANARYAQGLGSQIELSDAETAVTTAQGNLVQSEWQLANAWTN
                     LRRSLGER"
     gene            complement(14047..15282)
                     /locus_tag="VFV99_03260"
     CDS             complement(14047..15282)
                     /locus_tag="VFV99_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5058347.1"
                     /translation="MSIFTTLKLAFRALWRNKVRSVLTMLGIIFGIGTVIAMISAGQG
                     ARQAVADVFSSMGTNLLIVTNGSSQSGGARGGAGTQMALTWADLEALQNGEVPTARYV
                     APVLTTKVQIAAEGQNWNTSVTGTTPEYFKIKNWDAKEGTLFDDDAAAAGPKVAVIGT
                     TIVTQLFAGTNPIGQAITISGQPFEVVGVLASKGQSAMGQDQDDVVIIPLKAYRSKLD
                     SGVGKYITRGQIYVSVKTEDDISRGEQQIAALLRQNHKLNATDDDDFRIRNLAELALN
                     RKASTDKVTTLLAIVAMMSLVVGGIGVMNIMLVSVIERTREIGIRMAVGAKPIDVMTQ
                     FIVEALVLAIVGGILGLGFGAGLAKFMAGYYGWNFTFPASTAAIAFAVAGGVGVVFGF
                     YPAMRASQLDPIQALRYET"
     gene            complement(15279..16022)
                     /locus_tag="VFV99_03265"
     CDS             complement(15279..16022)
                     /locus_tag="VFV99_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5058348.1"
                     /translation="MLVDMRGIERTYIMGDNEVRALRGVDLQIEKGASLAIMGTSGSG
                     KSTMMNIMGCLDQPTKGQYFLNGKDVSRLSRAQLADVRGRMIGFVFQSFNLLSRTTAL
                     ENVEMPLMYQGVPGRQRHKRAMEALERVGLHNREHHHPNQLSGGQQQRVAIARALVCN
                     APIIMADEPTGNLDTKTSHEVMAILQGLGDQGITVILVTHESDIAAHARRVITLRDGL
                     IVDDKTQEPVRAGDGGAATGARPQEGSAA"
     gene            complement(16022..17380)
                     /locus_tag="VFV99_03270"
     CDS             complement(16022..17380)
                     /locus_tag="VFV99_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5058349.1"
                     /translation="MKLKRWIPRIILLAVVAGGVAFLIIKKPWAKSAPEITFSTVNVG
                     KGNIAAQVTASGTLSAVGTVQVGAQVSGRVVELHADFNEKVKKGQIIAKLDDSVLKAQ
                     IEQSQAAYNLAVANLNKAKVTAANAKRQLDRQKSLQEQQLVAGATVDQFQTDYDTANV
                     SVSASQADVSRAAAALKQARTNLGYATIYSPVDGVVLSRAVELGQTVASSLQAPTLFT
                     IAEDLARMQIDTAVAEGDVGALKESMKATFTVDAFPGKTFEGTVRQVRNAPTTNQGVV
                     TYDAVIDVDNKELNLRPGMTANVTFVLSQVTDAIKIPNAALRFKPSRDQMMAIFDKFG
                     GGRGRNGSGGSGGSGGGRRGRGERGSGGGSATDGSGGSDRPRAADAVTGASGGGMRGQ
                     GDRKPVWKLVDGKPKMTYVKTGLTDGSSTQMLEGDLQPGDQLITEIQGLPTQSRGRMG
                     AF"
     gene            complement(17377..18678)
                     /locus_tag="VFV99_03275"
     CDS             complement(17377..18678)
                     /locus_tag="VFV99_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5058350.1"
                     /translation="MSRRRLALSLYALTAAMSIAIMVALLVLPRFVGSPRYLEPQAAL
                     VQYMVDRLSTRDPQRLAERMDRIGKRLKGKLTLYDTRGTVLRSTAEPLPVATKGELKT
                     LETEKWALAWRRIVVRSDDATMIGVYVPNSPGFPWGFVLPLGAGVLLVVGAATFWFSR
                     RLVKPLGTLATAARNFGGGDISARAKLRRDDELGDVGRAFDDMADRTSAMISAQRQLM
                     GDVSHELRTPLARIRVALELAAEDPVAAKDVLADVGRDLDEIDQLIEDILTTARLDGE
                     HTDVKREPISVGELADRAAARFSSRHPGRVLEKTVDDSERAIECDPVLLRRALDNLLD
                     NAAKYSDAAVELTVHPNGKQIEFSIVDRGVGMTSEELARAGTPFWRADGSRARRTGGV
                     GLGLALARRIARAHGGDVSLDSAPGRGTTARLQIPLLESAS"
     gene            complement(18671..19381)
                     /locus_tag="VFV99_03280"
     CDS             complement(18671..19381)
                     /locus_tag="VFV99_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5058351.1"
                     /translation="MSEAPATIKVLLVEDDSRLAQLTSRYLEGHGVVVTLAADGVEGQ
                     NEALRRQYDCIVLDLMLPGRDGIEVCRQLRSRSDVPIIMVTARGEESDRVLGLEVGAD
                     DYVTKPFSPRELLARIKANVRRVRGQAGPQQELITVGGLSLDPAKMTVTLDGNPIEVT
                     AYEFAILRALAQRPGKVLSREQLLDLAKGSADLSFDRSIDVHVSRLRAKLGDDSRSPK
                     ILKTVRGVGYLLAGGGHE"
     gene            complement(19421..20965)
                     /locus_tag="VFV99_03285"
     CDS             complement(19421..20965)
                     /locus_tag="VFV99_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058352.1"
                     /translation="MSRETELEGRIAALEAELRQAREQRETTASILDLVLRQMPAIYW
                     IVDRDLRLLRTGGAIMEVLGYDQNRYVGRRLQDAIADDPSTNDTVATHTRACNGETVT
                     YEAEYRAKVLATVIAPYRNATGEIIGAIGTCLDVTVWRALERRMVDAQRAESLGVLAG
                     GLAHDFNNLLVAVLGNADIAMREQAEGKNARSTLENIRTAGLRAAELTEQLLAYAGRG
                     GAGTIRVEPALVVEELLRIISPSIPSEVRITVEIPTKLAVRGDPAQLRQVMLNLINNA
                     RDVLQGRGGEIAVRAAIEEISGEPHPHDVLTAPAGTHVVIEVEDDGPGMDADTRRHVF
                     EPFFTTKNRGHGLGLAAVLGIVRAHGGGIRVISAPGTGAKFQVLWPADAGTATRASTN
                     GSAHGRTVLVIDDEALVRDVVARMVEDLGYDAITAADGPAGLDVVARQAVDVVLVDLT
                     MPAMSGADVVAALRERKPDLPVVLCSGYDRDGRGPVFADAYLPKPFRIDALERTLAKL
                     LPLRSV"
     gene            complement(20975..>21466)
                     /locus_tag="VFV99_03290"
     CDS             complement(20975..>21466)
                     /locus_tag="VFV99_03290"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5058353.1"
                     /translation="EIVDQIVETVVAIRTRQRRARRVIVIKAEGVQMQVDKLKTEVDA
                     RLRERHPDAEPSTIETRVTVLGHVVRGGRPSAFDRLLGSRLANVALRGLLANATHKMA
                     AWMPPVELPEEVGMRSPYDPYCWLVDLGAVLAETENLLKGRSPLARWRAGAFEEIEDA
                     LLL"
BASE COUNT         3686 a         7068 c         7235 g         3477 t
ORIGIN      
        1 atcgtgatca cggcaccgag ccacggcgtg ctgccgacga cgaccgcgca tgcgccgatc
       61 gcagcggcgc cgttgcgcgc atcgttgtca cgtgcgtagc gtgctgcgaa cgcgacgctc
      121 cacaccgcgc cgcacgcggc aaggatcgca ggcgatgcaa acgcggcggc gtcccggaat
      181 ggcggcgcga tcaggagcag cgcgaccgac acgagcgcgg ccccgacatc gcgcggcacg
      241 agcgcgcgca ccgcggcgac gacaccggcg agcgtgaacg cggcgagcac cgcatcgagg
      301 atcgcgagcc gaaacccgac ttcgccccac ggtaggagcg ccgcggccga tgcgagcacg
      361 ttgtagagcg ggacgctcgc gacgtgcatc aggcgcgccg cgccgacagc gttggctgcg
      421 gcaacattcc agcccggacc gccacccgcg tcgagcacga gcacgacgag cgcgacggcg
      481 agaacgatcg tctcgagcct tcccgtctcg agctgcggaa gcttccggtc tggcacggcg
      541 ccaaggctgc cacagacgcg atatgatgac cgcaggcatg gcccagatcc ggatcgtcga
      601 cgactggttg cgcagcccgg cgtcgccgcc cagtcattac cgatccgtga tggtgatcgc
      661 cgagaagtgg gccgagcagc gcgtgctcat gcgtgcgtat gcccagagcc gcgatcagac
      721 gatgccgacg atcatgctgc acgcggtcga gctcgcgatc ggtccggccg gtctcgacgt
      781 caacggaccc tggggcatcc acgtcggctc gaacccgggc atggaggcgc aggcggtcaa
      841 agccggcctc gaagatgccg cgcgccggat ggccggttcg aaaggaaatc cgccgcgcct
      901 cgtcgacgag tcgaacacgt tcgatcgcga accgaccggc aactgggcgc cgggcacgcc
      961 gcgcgtgctg ccgcaacgtc ccgatcagcg cgcgcccgcc gtcgagcatg tcgctgccgc
     1021 cgccgccgct cgcgttccgc aggcgcagat ggcgacctac attccgcaag cgccagccgc
     1081 gcagcatgtt ccgcaggcac aaacggccgt cgtcgcgccg tcgaatcccg agttgcgcaa
     1141 aacccccgtc ccgcggcggt cgagcggcaa gacacggccc ccgccgacga atgtgcgaac
     1201 ggcgctcggt tatacgtcgg gctccggagc gcagtcggcg atcgtcaagc tagggctcgc
     1261 tccgcagatt tcggcgcggc taggccggct cgtcgatcgg gtggtccctg cggatttctc
     1321 gatcgatgcg ggcgagcgcc gggtcctcaa cgcgctcggc gaacatgcac ttactgcacg
     1381 ctcgatcggc cacatgctcg atatcgcaga tgcgacgacg ttcatggaag agctgacgtc
     1441 caagctcgag cgctacggcc tcggggacct gatcgagccc ggtgaaccgc aaggcggtga
     1501 gccgacgtac cgcatgcgcc gctagtgttt cccaggggaa ggccctgtgg taaccagtcg
     1561 tgcccctatg caggtacgca ttgaagaagt gtctcccgtc gagaagaaga tgttcgtcga
     1621 gatcccgtgg gagacggtga gtcagaaact cggcgatgcg taccgcgagc tcagcaaggg
     1681 cgtcgcgctc aaaggtttcc gcaagggcaa ggtgccgcgc ccggtgctcg agcaggtcta
     1741 cggcccgcgc gtgaacgccg aggtcgccta cgagctggtg cgcgagtcgt tcttccgcgc
     1801 gaaccaggag cacaacctcg ccgcggtcgc cgagccgcgc gtcgaagagg ccgatccgat
     1861 caagaagggt cagccgttca agttcgccgc gatcatcgaa gtgcgcggcg aagtcgtgcc
     1921 gcaggactac aagggcctct cgatcgagcg ccgcaagctc aacatcgcgg acagtgcggt
     1981 cgaggagtcg ctcgcccagc tccgccgcga gcacaccgag ctgcgtccga tcgaaggccg
     2041 cgaggtgacg gctgcgggcg acatcgttgg cctcagcatc accggcacga tcggcgagca
     2101 ccagatcaac cagccgcagt tcgcagtcga tctcgatgac gtcgagcgcg agccgctgcc
     2161 gggcatccgc gaggcgctgc tcggcattcc gctcgacacc aaagagaaga agctcgagct
     2221 caccgttccc gatgactaca aggacgagtc gctgcgcggc cgcaaagccg atctgacggt
     2281 gacgatcctc gaagcgcgcg cgaaggacgt gcccgagctc gacgacgaat tcgcgaagga
     2341 caccggcaag gccgacaccc tcgacggcct caaggccgcg ctccgcaagg agctcgagga
     2401 tcgcgagacc gaggtcatcg atcgcgaggc gcgcgagggc gtgctgcgcg agctgatcaa
     2461 gaagaaccag atcccggtcg cgtcgtcgct cgtcgatcgc gcggtcgaga tgcagtacca
     2521 gcgcctgcgc cagatgctcg gcatgaagcc ggagcgcaac gcgcagattc cggacgatct
     2581 caaagacaag atgaagccgg ccggcgccga cgaggtccgt ggccagctgc tgctcgaagc
     2641 gatcgccgac aaagagaacg tcgtcgtgac cgacgaagag ctcggcaagc acgtcgagca
     2701 gaccgcgaag gcacgcaaca tggcgcccgc gaagctgcgc gccgagtggc agcgcgacgg
     2761 tcgcctcgac agcgtcacgt actcgctgcg ccaggacaag gtgctcaagt tcctcgtcga
     2821 tgctgcgcag atcaccgagg tcgagaagct cacgcagccg ggcacgcccc tgcccgaagg
     2881 tcccgcgcac ggcgaagagg gccacgtcca cggccccgac tgcgatcacg accacgacca
     2941 ctgataaatt tgcgcgacgt agagcgctgg ttttgctggc tggagcggag gcgcgcgtcg
     3001 tgaagcacat ttcgggcgtc gacgggaggg tggcgttaca ttcaccggca tggagagcga
     3061 caagacgcag ccggggatga gcgggccgca cccgaccatc gcccagttgc gaaatccaac
     3121 cgagcccggc atcgacgacc acgtcgcgtc gtgttcgatg tgccgctcgg tgatggcggc
     3181 cgacgtcgac gagacgccga tcccgctcga gctaccaaag ggtctggtca gcgagaccgc
     3241 gttcaagtgg ccaccggatc cgatcgcgcg tggtggcatg gcggcgatct tcgcgggtga
     3301 agatcggcgg ctcggtcgca cggtcattct caagacgccg cgcgaagacg agaacctgcc
     3361 gaagtacatg accgacatgt tccagagccg cgtcaccgcc gaagcgcgcg tgctcgcgaa
     3421 gctgcagcat ccgtcgatcg tcacgatcta cgagctcggc aagtcgagcg tgggctggcc
     3481 gttctgcgtg ctcgagcgag tcgatggtac gtcgctacgc gagcgcctcg acgagctcgc
     3541 cgacgccgaa gaaaaactga tgaagccggc gacgcgcgag cgtctcgagc tgctctcgaa
     3601 cctcgtctcg atcgccgaag cactcgcgta cgcgcacgag cgtcgcgtcg tgcatcgcga
     3661 tgtcacgcct aacaacatcc tgctcggtaa gcgcggcgaa gccacgctga tcgactgggg
     3721 catcgcgcgt gatctcgacg cgcctggcgg taccgagccg tcgattcatg acgaggaccc
     3781 gtcgacgacc ggccgcatgg tcacgatcag cgccggcacg ccgccgtaca ttccgctcga
     3841 gcagagccag ggccgcaaag ccgatccgag cttcgacgtc tacagctttg gcgtcacgct
     3901 gtacgaggtc gtgtcgggca agacgccgtt cgtgtggggc ggagcgacct cgccgggcgg
     3961 ccgcaccgcg caagtcgacg cgttcatggc gtggctgcag tccgacgatg acatcccgcc
     4021 cgcgatgccg aaggataccg agctctccgg catcatcgcg aaggcgatgg ctcgcaagcc
     4081 cgccgatcgg ttcaccgccg acgagctggt gcgcgcgctc aagcaatacc tgaccggcga
     4141 cctcgtgttc tcgcatcgct actcgctgtc gggccggctc gcgcggtggg cacgtcgcca
     4201 caagaccgcg acggtgtcga tgctgttcct caccgtgctc gtcgtcgccg gcgcgcttgg
     4261 atgggccgcg tggtcgcgcc agcaaaagga agaagccgag ctccgtgcgc tcgcggcgtc
     4321 tgcacaggtc gacgcgagcg agaaaggtcg cctcgccgac gaagcgagcc gcgaagccga
     4381 cgccgcgaag gcgctcgccg agcaagcaca gcgcgaaggt aaggacgcgc aggcgctgaa
     4441 gcgcgaagcc gatcgcaagc gcaaagccgc cgaggaaacg cgccgcgctg ccgaagctgc
     4501 cgcgacccaa gcgaagggca ccgccgagga cgcgctcaag cgcttccagc aggcgatgaa
     4561 ggaaaaggac gaagcgaaca cgcaacgcga cgccgcgaac agcgcgcgtg atcaagccga
     4621 gcgcgaacgt gaagccgcgc gcgcgtccca ggtcgccgcc gagcgcgatc gcgatgcgtc
     4681 gaaagccgca cgtgacgccg ccgacaagga gcgcgacgcc gcgaagcaaa ccgcagcggc
     4741 cgctgaagcc gagcgcaacg cggcgcgtgc cgcgcgcgac gcctcggaca aggagcgcga
     4801 tgccgcgcgt gccgcacagg ccgcctccga gaaggagcgc gacgctgcga tcgctgcacg
     4861 ctccaacatc gagaaggaac gcgacgaagc gcgtcgccgc gtcgccgacc tcgagcagaa
     4921 gctacgcgag ctccagcagg gtggcccgcc cggtccgccg cctccgccac cgtcgccgta
     4981 acgctggcgc ccacgccgat cgcggagcag agtgcgcgat ctgcgcgccg ttgattaacg
     5041 gacgtgtgcc ggcacgttgc ctgtgctggc atgaatcgtt catcaggacg gaggcgcacg
     5101 aacaaccgtc cgaggtgaat ccatgttggg aagactcact ctgtcgttgc tcatgacgct
     5161 cacgatttcg agcgtcgtgg catgtactga cctcacgagc gaggacgaag agaccagcaa
     5221 tctctcgagc gaggagcagg cctgcatgca tgacgaggcc gcaaagctcg cagtgctaaa
     5281 gaagctccac gaccgctacc aagacatcaa cgttgcgctc gccgatggtt acaagctcgg
     5341 tgtcatctac aacggcaatc tcgtcgtcaa cggctgcgta tcgaatctag ctaatccggc
     5401 gctcggcgcg atgggctatc actacttcct acaagaccgc ttcgacgacg cgaagatcaa
     5461 agagctcaag cccgaggtcc tcgtctatca caccggcgca aacggtacgc gcgtgctcgg
     5521 cgcggtcgag tgggtcgtgc cgaagccagc ctgggacgcc aaacacggcg tcgatgccga
     5581 cgcacccgag gtgtacggac agatgatgat gatcctcaat ccgatgctga actggtacgt
     5641 ggcgcacgcc tggctctgga aagacaatcc gtccggcgtc ctctcggact ggaacccaga
     5701 cgtgacctgt ccctgacgac acggcgtact gacggcgcac gttccggaac gccagcagtc
     5761 tgtgcagcgc tacgtcgtcg gccgcacgac tttgacgggc gccatgctgt ccgtcttgtt
     5821 gccgtcgccg agctggccgc gatcgccgcg gccccagcac cagatatcac cgtcgacgag
     5881 gagcgcgcag ccgtgcgcga agccgactgc gacgctcgtc gcggcaccgg gtaatccgag
     5941 cacatcgacg ggcgtcgact cggatacggt catgccgtta ccgagacggc cgagctcgcc
     6001 gcggccccag cacgagacgt tgccgccgct gatcgcgcag ctcgcatcgt gatcgacagg
     6061 accacctcca gtctcgagtg cagtgacgtt ggagagacca ctgaccatga ccggcgtcgc
     6121 cgagttgttc gtcatgccgt tgccgagctc gccgctgttg ccttcgcccc agcaccacac
     6181 ggtgccgccg acgaggccgc acgcgtgcca gcccgcgatg cgcggcagcg acgtgatgcc
     6241 gccgatcacg ccaacgggat cgaccgatgt cgtcggtgac atgctgccag tgccgagcgc
     6301 gccgctgtcg ttgtggcccc agcattgtgg cggttgaccg ggcgcaaatg cgcaggcgtg
     6361 atcgtcgccg gcatcgatct gcgttgcggg gtaggcgggg atgaagttga cggacgttgg
     6421 cgtcgcaggt ggagccaccg agcccgtgcc gcactggccg gtatcgtttt gtccccagca
     6481 ccgcgtcatg tcggcgatca cgcacgagaa gttttttccg gcggcgacag cggtcgcggg
     6541 cgcaccgagc gggatctgcg ttggcgtggg gccggtcggc gtgccccagc aatagagatt
     6601 cgcgtcgacg atcgcgcagc cactgacgtc accttcggag agagcgttga cggagcctgc
     6661 tggcagcagc acgcgtgatg gggtgatcgc gtcgttgttc gaaccgtcgc cgatctggcc
     6721 gttgttgttc gcgccccagc aataggcggt gcctttgtag agcgcgcacg tctgatcgcc
     6781 gtaggcgacg agcgagctga acccggagcc gttcggtccg tcgccggagc cgggcgcgtc
     6841 gccgccgttc gcgttgagct ccgcgaaatc gatgcggccg catgcggcga gcgcgcaacc
     6901 accgagcacg agacaacaac caacacgcca cacggttgcg attgtctcag agaacggcgc
     6961 ccatccactc cgcaatcgat cggtacttgc cgtcgccgac gacaacgtgg tcgatgatgg
     7021 gtataccgat cacatcaccg accgagcgca ggcgctcggt cagcgcgatg tcctcgggac
     7081 tcggcgtcgg atcgccggtg ggatgattgt ggacgagcac ggcgcccgcg gcggccatgc
     7141 gcacgagcgg ccgaaacact tcgcgtggat gcacctcgac ggcatgcacg ctcccacgcg
     7201 cgacctcgac gacatcgagc aggccattgc ggatgtcgat ccccatcacg atgaagactt
     7261 cttgggcgag accggcgagg cgtggaccga cgatgccgaa cacgtcggcg ggatgaccga
     7321 ggatcgtgcg gtgttgctcg ctctcgagcg cgcggcggcc gagctcgaat gcggccgcaa
     7381 tgcgcgtggc gcgagccgtg ccgacgccgt tgacgagtgc gagctcgcgc ggtgatgcgc
     7441 gtgacagtgc ggcgacgctg ccggttgctt tgacgagctc gcttgcaact gcgagcgccg
     7501 gtcgatcgcg cacaccggtg ccgagcacga tcgagaggag ctcttcgtcg ccgatcatcg
     7561 tggtgccgcg actccacagt cgttcacgcg ggcggtctac gagagtcatg gcgtaacgcg
     7621 taggcaagct gcgcgccacg tggcgagcgc gaggagtcgc atggttgcga atcgcggtgg
     7681 ccggtgaccg gtcgctggcc accgagattc ggacacgact ggccggacag cgccgagcag
     7741 gcggcggcgg cagcggcggc atcatgacga ccgcgtgtta ggctctgcgg atgttcgtgt
     7801 gcgcggagtg cggtgcgtcg cagccggggc ctggaccgtg ccgcgccgac ggcacgccga
     7861 tgatgccgat cggcgacgac ctgctgctcg gcacgacgat cggcgcgtat cgcgttgcgc
     7921 gcttgctcgg cgttggcggc atgggccgcg tgtacaaggg cgtgcacccg acgatcggca
     7981 gccgcgtcgc gatcaaggtg ctgtcgcgcg agtgcagcga ccggcgcgac ctcgtcgctc
     8041 ggttcttctc cgaggcgaaa gcggtgaacg tgatctctca cgagagcatc gtcaacgtgc
     8101 tcgacctcgc gacgctgccc gatggcaggc cgtacatcgt catggagtat ctcgacgggt
     8161 cgccgcttgc cgggatcatc gagcacgcgc agcgcagcgg tatgccggtt ccgctcggcg
     8221 gcctcgcgcg actcgtcgcc gaagtgctcg acgcactcgg cgctgcgcac gcgaagggca
     8281 tcgttcaccg cgacctcaag cccgacaaca tctacgtcac gcctgcgggt cgcgcgaagg
     8341 tgctcgactt cggcatcgcg aagctaatgc agcccgtggg cgaagcggcg agcggcagcg
     8401 ccacgcatac cggctcgttg ctcggcacgc cacattacat gtcaccggag caggcagcgg
     8461 gccggccggt cgacgcacgc gccgacctgt acgcgatcgg cgtgatcttg ttcgagtgct
     8521 cgacgctgca gaagccgttc atcgcggact cgttgttcga cctcttgcgc aagcacgtcg
     8581 aggcaccgcc gccgtcgccg cgcgcgttgc gtcgcgacat gcccgaagcg ctcgagcaca
     8641 tcatctatac ggcgctcgcg aagctgcccg accagcggtt cgcatcggcg cacgcgatga
     8701 gcatcgcgct gcagcacgcg acgatgaatc tgccgagcga gcagtggacg ccgattcaac
     8761 ccgcgagctc gagtcgcagc gcaccgtcgg gcggctggaa tccgacgcca ccggcgagct
     8821 gggcgggcgg caatcgaccg agcgtcgcgg tcgcgccgac acagacggca ggacaggtca
     8881 cacgaggaga gcggggcggt tcgaagaagg ggttgtggct cgcgcttggc gcgttggtcc
     8941 tcgtcggcgg cggcatcact gcggcagtga tcgcgggcgg cggcaaaggt gacaagcagg
     9001 tcgtcgcgca aggctcgggt agtgaggcgg ccgcatcggg cagtgatgcg tgggcgacac
     9061 caggaagcgc cgcgcgcgat ggggtgacgc caccggtgac gcctccggtc gagcacgagg
     9121 cgccggatcc cgacgacgac gcagacgacg gcgagctcga tgcagcgtcg accgaagcga
     9181 tggaccaagc attcgccgag gcattcaaca acctcgatcc ggctgcgcgc gcgatgttcc
     9241 cgcccgaggt gaacgccgcg ctcaagaaat acggcaagtg gtcgaagatt ccgaaggcgg
     9301 agcgcatgaa gctgctcgcg aagatcgcgg gcatgacgca aaacctcacg aagtctagcc
     9361 gcgacatcaa tcgcgcgctt gcgggcgatc ccggcagcac ggctccgaca ccaccgacga
     9421 cgccaccgac gacgccgaag acgagcggag ggcctgtcgc cggtctcgac gcgaacggct
     9481 gggtcgtgtc gcgccggttc aatccgtccg gctacaacaa gaagaacctg tcggtcgaga
     9541 agtatctgcc gacggcgatc gccgaagcga agaagctcgt gcccgacgcg gtgctgttcc
     9601 gcatcgacgc cgacggtgtc tatcccgacg gtcacgccga catcacgctg gccgagcacg
     9661 gctcgctcga cttccgcttc atctcgcaaa agcgcgcgaa gggcgatccg aagctgccgc
     9721 tcggcgcgaa gcaggaatac aagtgcatgt tccgcatcat gttcgacgac gagggcggct
     9781 ggtccgcgcc gatcgacggc tgggagtgca aggagacctt gatcggatcg ccgaagtgca
     9841 cgaccgcgca ggtgtggaag aaggcgatcg cgaaaggcgc gccggcaaac gcgatcgccg
     9901 agctcggtta ccgcgcgtgg gacggcaagg cgcgctggta cttcgacatc ggcggcacga
     9961 agttcagcga agtgttcgac gacgactgcc gctaagggag cacgccgaac tggccgcgca
    10021 gcgtgaagtg ctgcggtgcg accgatagcg acgggctctt gatgccgagc tgcccgccga
    10081 ccggcagacc gtattgaccg agcgacgccg gaatcgtgaa cgacgggatc gggatcgcgg
    10141 gcagcgcgtt attgagtgac tggttgacca gctgctgcag cagcgtgtta agcgcggtct
    10201 gcaggctctg ctgctcggac gcgtcgaggt tcacgtcgtc tgtgccgacg tgcgtctgat
    10261 cgatcacgat gttaccaaac acgaggtcat ttccgacgag cgtcaccgat gcgtgtgcat
    10321 cggcgcccgc gcgaaccgcc aggttcacgg gcaggaacgg atgctggatc acgaggtcga
    10381 tcgcgccgag ctgtagctgc acggtgccgt tgccgaggat cgtcgcgacc ggtggcagcc
    10441 gcgttacgat ggagatcgtc gtgcctgcgg gcagtccggc gccgagctgg gcgccggtga
    10501 gcgtcgctgc gaggtagttc gcgcgccaca gtgcgtgcag cgcgtgctcg atgatgccga
    10561 tatgcgcgcc gacacctgta ttgctcggcg ccgatacggt cggatcgagc aggatcgtgc
    10621 cggtgggtag cgggaccccg agcgacggat aggcgttcgc tgccggtgcc gtgaacttgg
    10681 tgctgatgcc gaacagtgcg cgcgacgtcg tcgtgctcag cgacgagaac cccacgccaa
    10741 agctgagcgg tacgttgccg ctgccgtcga ggcgcggaac gttgaacgag gtgccgagcg
    10801 tgctgatgtc gaggttggcg acgagaccat cgaggacggc gttgaagttg ttcgtgacga
    10861 agttcgtgat caggttcgtc agcgtggttc gcagcgagcc ctgcgccagc ggtacgacga
    10921 tgttgttgat gatccatccg tcgacgccac cgaatgccgt cgagatcgag ccgacggccg
    10981 acgagaccga acccggtcgc accgagatgt ggggcttacc gcccgagatc gacaggtcga
    11041 gcgtcagcgt gacatcgatg tagctgaccg tgacccagcc cgaggtgtcg tatccgatcc
    11101 cgctgacagc gccacgtacg cgcagattga cgccgatgtt cggaatgtgc gcgtgcgcga
    11161 tgatgccgtt cgtcgtcagc gtgaggtcga cggtctgcgg accaggcaag ctgagccctt
    11221 gatagtcgat gcggctcgag tacaagcagg cacagccgat cagcgggatg cagacctgtg
    11281 agtcgcagct ctgcggcttg agcggattgg atgccttgag cgagctgtcg atcgtggtcg
    11341 cgaggcccga gctgttgaga acgacgtaga gcacgtccgc gagcgagttg atcgcgccgc
    11401 cgcggttgcc gtcatcgacg gcggcttgcg tgagcttgag cgagatctcg tcgttgatca
    11461 ggtttgttgg acttccgtag gtgttcgaga cgagataggt gcacagcttc gtcgtcggct
    11521 cgccgaacgc atcgagcgcg gtgacgtcga cgaagttgag gccgaaccgc gtcacgatcg
    11581 gcgccgagaa cgcgccattc gcgccgaccg caaccgagtt gccgttgacg ctgatcgacg
    11641 agacaccgtt cacgtcattc gcctggccgg tgaccgtcat cgtgctacct ggcgtgaggt
    11701 tcaccatcgt cgcgtcgccc gcgcacgtga cagcggggcc acggctgttg acgacgacat
    11761 ccgtcgtcgc gctgagcggg acgttgttgt cggtcggcgg agtgaccgcg acggtgatgc
    11821 gatagaggcc ctcgccgttg tagcggaacg tgttcgcgtt cacgatcatc gtcgggccga
    11881 cacccgtgac cggtgcgctc gtgttcgtga cgggcggagg agcgatgtcg ttgccgtagc
    11941 ggtcagagac gaccgcatcg acctgcacgg tctggccgac ggcgtagacc gggaggtcgg
    12001 gaaacttcga gatcgcgaga ttcgcgggca ggttcgggac gacgtcgaac gccgcgttgt
    12061 tgctcgtcgt gccgggcaga tagcagcggc cggtgtaatg accggcgcgc gtcatcagcg
    12121 cttgcagatc ggtgatcgta ttcgccgagt catcgggcgt cagctgcagc gtcggcatcg
    12181 tgccggtgac gacgttgccg taggcgtcgt agaccacgca ggtcgtcgtg atcgagttac
    12241 cggcgacgac cgggtcggga acgatcgtgg tgacgaggtt cgccgccgcg cccgcgacga
    12301 tctcgaccgt ggcgggcgtc gtgtcgacga tgccgcgacc gggcagcacg catgcgatgt
    12361 cgacgctgcc ggcggtcttc gcgatgatct ccgcgcccat tcgaatcacc gacgtctctg
    12421 ccgtcacggc gatctcgccg gtgaccatcg actcgatgtc gttctcggtc agcgtgcacg
    12481 agacgttgat cggatcgccg gcggcgactt gcgccggcgc gtgcgtctcg agcttcacct
    12541 gcacgatggt gtcctcgtcg ggctctggtt tcaggttgtc accgcaggcc accaacagaa
    12601 tcgcgaatac gacaagacga cgccccatcg cggtccagct tatcagcggg tgtcgtacgc
    12661 ccgcgtacta aaatcagcgc tcgcctaacg atcggcgcag gttcgtccag gcgttcgcaa
    12721 gctgccattc cgattggacc aggttgccct gggcggtcgt gacggccgtc tcggcatccg
    12781 acagctcgat ctggctgcca agcccctgcg catagcgcgc gttggcaagc ttgagctgcg
    12841 cgtttgcggc gtcgacggct tccttcgatg actcgacacc tgcgcggttc gcttcgatct
    12901 ggctgcgcgt ggcatcgagt tgtgaagtca aatcgatgag gagcgcgtcc cgctgcgcga
    12961 tcgcagactg cttgttcgcg cgggcgatcc gcacatctgc cttcgtgcga ccgccatcat
    13021 agatcggcca tgacaacgtc aggccggccg tccagccggg atgctcccag ttgccgccga
    13081 actgcggcga ccagtcggcg cgcgcagcag ccgacagcac cgggcgcaac tgtgcggagg
    13141 cggcgtcgat cgaggcatcg gacgcagcga cctccttgtc cgcctgcacg agctcggggc
    13201 gctgcctgcg cgccgtctcg acgagcgagg tgagcggcgg cgcgtcgccg ggcggcaccg
    13261 gccagctgtt cgcgacgacg aacgactggt tcggatcgac cgcgccgatc gccgcacgca
    13321 gcgtcgcgag tgcgatcgcc tgattcgaat aggcgagcgc tagcgcggca cgcgcgttcg
    13381 cggcgcgcgc ttgggccgtc gcgacttcga tcggatcctt cgcttgcgcg gcgacgaagc
    13441 gtttcgcttg atcgacgtgt gcctcttcgc tcttcacggt ggcctcggcc acggtgacca
    13501 agcgcgcacg tgcgacggcc tcgagatagc tgagctcgac gttgcggacc acgtcgagca
    13561 tcgtcgtgtc gaggccggcg gtcacagctt ccgcgttcag cttcgctgca tgcagctgcg
    13621 ccgcggtctg cccgaagtcg tagatgcgcc acgaggctgc accgccaacc gatgcaccgg
    13681 tcgtgggatc gaggaaatct ctcgcgaggg cgccgctgcc accgccacca cttgcggtga
    13741 ggttgccagt caccgtgagc gtcgggcggc gggtggcctg cacttgatcg acgcggccac
    13801 gagcgacctc gatcgacgcg cggctctgcc gcaacgacgg gtcttgcctc atcgcgagtg
    13861 ccaccgcttc ggccatcgtc agcgtctgcg cgttgggcgg cggcggcgtc ggcgcgggcg
    13921 gctgcacgaa ctgcggtttc ggctgcggct gcgcgggctg cggctgcggg ggctgcggtt
    13981 gcgtgggctg cggctgcgcg aatactggcg ccgagacgaa gagggcgccg agggttacta
    14041 ccaacttcat gtttcgtacc tcaatgcctg gatcgggtcg agctgcgatg cccgcatcgc
    14101 gggatagaac ccgaacacca caccgacgcc accagcgact gcgaatgcga tcgcagcggt
    14161 gctcgccggg aacgtgaaat tccatccgta gtagccggcc atgaacttcg cgagtccagc
    14221 accgaagcca aggccgagga tgccgccgac gatcgcgagc acgagcgcct cgacgatgaa
    14281 ctgggtcatc acgtcgatcg gcttcgcgcc gacggccatg cggatgccga tctcacgcgt
    14341 tcgttcgatc acgctgacga gcatgatgtt catgacgcca atgccgccga cgacgagcga
    14401 catcatcgcg acgatcgcga gcagcgtcgt gaccttgtcg gtcgatgctt tgcggttgag
    14461 tgcgagctcc gcgaggttac ggatgcggaa gtcgtcatcg tcggtggcgt tcagtttgtg
    14521 gttttgacgc agcagcgctg cgatctgttg ctcgccacgt gagatgtcat cctcggtctt
    14581 gaccgacacg tagatctgac cgcgcgtgat gtacttgccg acgccactgt cgagcttcga
    14641 tcggtaggcc ttgagcggaa tgatcacgac atcgtcctga tcctgaccca tcgccgattg
    14701 gcccttcgac gcgagcacgc cgacgacctc gaacggctgg ccgctgatcg tgatcgcctg
    14761 accgatcgga ttcgtgcccg caaacagctg cgtcactatg gtcgtgccga tgaccgcaac
    14821 cttcgggccg gccgcggccg cgtcgtcgtc gaacagcgtg ccttctttcg cgtcccagtt
    14881 cttgatcttg aagtactccg gcgtcgtgcc ggtgaccgac gtgttccagt tctggccctc
    14941 ggcggcgatc tgcaccttcg tcgtgagcac gggcgcgacg tagcgcgccg tcgggacctc
    15001 cccgttctgg agcgcctcga gatcggccca cgtcagcgcc atctgtgtgc cggcaccgcc
    15061 acgtgcgccg ccgctctggc tcgagccgtt ggtgacgatc aggaggttcg tgcccatcga
    15121 gctgaacacg tcggcgacgg cctggcgcgc accttggccc gccgagatca tcgcgatcac
    15181 cgtgccgatg ccgaagatga tgccgagcat cgtcagcacc gagcgcacct tgttgcgcca
    15241 cagtgcgcgg aacgcgagct tgagcgttgt gaagatgctc acgcggccga accttcctgg
    15301 ggccgcgcac cggtcgcagc cccgccatct ccggcgcgaa cgggttcctg cgtcttgtcg
    15361 tcgacgatga ggccgtcgcg cagcgtgatc acgcggcgcg cgtgcgcggc aatgtcggac
    15421 tcgtgcgtga cgaggatgac cgtgatgccc tgatcaccga ggccctgcaa gatggccatg
    15481 acctcgtgcg atgtcttggt gtcgaggttg ccggtcggct cgtcggccat gatgatcggc
    15541 gcgttgcaca cgagcgcgcg tgcgatcgcg acgcgctgct gctggccacc ggacagctgg
    15601 ttcgggtggt gatgctcgcg gttgtgcaag ccgacgcgct cgagcgcttc catcgcgcgc
    15661 ttgtggcgct ggcggcccgg cacgccctga tacatcagcg gcatctcgac gttctcgagc
    15721 gcggtcgtgc gcgacagcag attgaagctc tggaacacga agccgatcat ccggccgcgc
    15781 acgtcggcaa gctgggcgcg cgacagtcgc gacacgtcct tgccgttcaa gaagtactgg
    15841 cccttcgtgg gctgatcgag gcagcccatg atgttcatca tcgtcgactt gcccgagccg
    15901 gacgtaccca tgatcgcgag cgacgcgccc ttctcgatct gcagatcgac gccacgcagg
    15961 gcgcgcacct cgttgtcgcc catgatgtat gtgcgctcga tgccgcgcat gtcgaccaac
    16021 atcagaacgc tcccatccgc ccgcgcgact gggtgggcag gccctggatc tcggtgatca
    16081 attgatcgcc gggctgcaag tcgccctcga gcatctgcgt cgacgagccg tcggtgaggc
    16141 cggtcttgac gtacgtcatc ttcggcttgc cgtcgacgag cttccacacc ggcttgcgat
    16201 cgccttgccc gcgcatcccg ccaccggatg caccggtgac cgcatcggcg gcgcgtggcc
    16261 gatcactgcc gccgctgcca tcggtcgcgc tgcccccgcc ggagccacgc tcaccgcgtc
    16321 cacgccgacc tccgccgctg ccgccgctgc cgccgctgcc gttacggccg cgaccgccgc
    16381 cgaacttgtc gaagatcgcc atcatctgat cgcgcgacgg tttgaagcgc aacgcggcgt
    16441 tcgggatctt gatcgcgtcg gtgacctgcg agagcacgaa cgtgacgttc gcagtcatac
    16501 cgggacgcag gttgagctcc ttgttgtcga catcgatcac cgcgtcgtag gtgacaacgc
    16561 cctggttcgt cgtcggcgcg ttgcgcacct gacgcacggt accttcgaat gtcttgccgg
    16621 ggaacgcatc gaccgtgaac gtcgccttca tgctttcctt gagcgcaccg acgtcgccct
    16681 cggcaaccgc ggtgtcgatc tgcatgcgcg cgaggtcctc ggcgatcgtg aacagagtcg
    16741 gcgcttgcag cgacgaggca acggtctggc caagctcgac cgcgcgtgac agcacgacgc
    16801 cgtcgaccgg cgagtagatc gtcgcgtagc cgagattcgt gcgcgcctgc ttcagcgcag
    16861 ctgcggcgcg gctgacgtca gcttgcgagg ccgagaccga cacgtttgca gtgtcgtaat
    16921 cggtttggaa ttgatcgacg gtagcgccag cgacgagctg ctgctcttgc agcgacttct
    16981 gacgatcgag ctgtcgcttc gcgttggcgg ccgtgacctt cgccttgttg aggttcgcga
    17041 ccgcgaggtt gtaggcggct tgcgattgtt cgatctgcgc cttgagcacg ctgtcgtcga
    17101 gctttgcgat gatctggccc ttcttcacct tctcgttgaa gtccgcgtgc agctcgacga
    17161 cgcggccgga cacctgggca ccgacctgca cggtgccgac ggcggacagc gtgccgctcg
    17221 cggtgacctg cgcggcgatg ttgcccttgc cgacgttcac cgtgctgaac gtgatctcgg
    17281 gggcgctctt ggcccacggc ttcttgatga tcagaaacgc aacgccgccg gcgacgactg
    17341 cgagcagaat gatgcgcggt atccaacgct ttaacttcac gatgcactct ccaacaacgg
    17401 aatctgcagg cgcgcagtcg tgccacggcc tggagctgaa tcgagcgaga cgtcgccgcc
    17461 atgcgcgcgt gcgatgcggc gagcgagcgc gagcccgagg ccgacgccgc ccgtacgacg
    17521 agcgcggcta ccatcggcgc gccagaacgg cgtacctgct cgtgcaagtt cttcggacgt
    17581 catacccaca cctcgatcga cgatcgagaa ctcgatttgt ttaccgttcg gatgcacggt
    17641 gagctccacg gctgcgtcgg aatatttcgc cgcgttgtcg agtagattat cgagcgcacg
    17701 ccgcagcagc accggatcgc attcgatcgc tcgctcgcta tcgtcgacgg tcttctcgag
    17761 cacgcggccg ggatggcgcg acgaaaaccg tgctgcggcg cgatcagcga gctcgccgac
    17821 gctgatcggc tcgcgcttga cgtcggtgtg ttccccatcc aggcgcgcgg tggtcagaat
    17881 gtcttcgatc agctgatcga tctcgtcgag gtcgcggccg acgtcggcca ggacatcctt
    17941 cgccgcgacc ggatcctcgg cagcgagctc gagcgcgacg cgaatgcgtg cgagcggcgt
    18001 gcgcagctcg tgcgagacgt cgcccatcag ctgccgctgt gccgagatca tcgcggacgt
    18061 gcgatccgcc atgtcgtcga acgcgcggcc gacgtcgccg agctcgtcat cgcgacggag
    18121 cttcgcgcgc gccgagatgt cgccaccgcc gaagttacgc gcagccgtcg cgagcgtgcc
    18181 gagcggcttc acgagtcggc gcgagaacca gaacgtggcc gcgccgacga cgagcagcac
    18241 gccggcgccg agcgggagca cgaaccccca cgggaagccg ggcgagttcg gtacgtagac
    18301 accgatcatg gtcgcgtcgt cgctgcgtac gacgatgcgg cgccatgcga gcgcccactt
    18361 ctcggtctcg agcgtcttca gctcgccctt ggtcgcgacc ggtagcggtt ccgccgtcga
    18421 gcggaggaca gtgccacgcg tgtcgtagag cgtgagcttg cccttcaggc gcttgccgat
    18481 ccggtccatg cgctcggcga gccgctgcgg atcgcgcgtc gacaaccgat cgaccatgta
    18541 ctggacgagc gcggcctggg gctcgaggta gcgcggactg ccgacgaacc gcggcaacac
    18601 gagcaacgcc accatgatcg cgatcgacat cgcggcggtc agtgcgtaaa ggctgagcgc
    18661 gagccgtcgc ctactcatgg cctccgccag cgagtagata gccgacgcca cgcaccgtct
    18721 tgaggatctt cgggctgcgg ctgtcgtcgc cgagctttgc ccgtagccgc gagacgtgga
    18781 cgtcgatcga gcggtcgaag ctcagatccg cggagccctt cgcgaggtcg agcagctgtt
    18841 cgcgcgacag caccttgccg ggccgctgcg cgagcgcacg caggatcgcg aactcgtacg
    18901 cggtgacctc gatcggattg ccgtcgagcg tgaccgtcat cttcgccggg tcgagcgaca
    18961 ggccaccgac tgtgatcagc tcctgctgcg ggccggcttg accgcgcacg cggcgcacgt
    19021 tcgccttgat gcgcgcgagc aattcgcgcg gcgagaacgg tttggtcacg tagtcgtcgg
    19081 cgccgacctc gaggccgagc acgcgatcgg attcttcgcc gcgcgccgtc accatgatga
    19141 tcggtacgtc actgcgcgag cgcagctgcc ggcagacctc gatgccatcg cgaccgggca
    19201 acatgagatc gagcacgatg cagtcgtatt gccggcgcaa cgcttcgttc tggccttcga
    19261 cgccatccgc cgcgagcgtc actacgacac cgtgtccttc gagatagcgc gacgtcagct
    19321 gcgcgaggcg cgagtcgtcc tcgacaagca gaaccttgat cgtggcaggc gcttcggaca
    19381 caccgagaag atacccatcc cggcgcctac cagaaggtcc ctacacactt cgtaacggca
    19441 ataatttcgc caaagtgcgc tctagcgcgt cgattcgaaa gggcttgggt aaataagcat
    19501 ccgcgaaaac cgggccgcgg ccgtctcggt catatccgga gcagaggacc acgggcaggt
    19561 cgggtttacg ttcgcgcagc gccgcgacca cgtctgcgcc gctcatcgcg ggcattgtca
    19621 ggtcgacgag cacgacatcg accgcctggc gcgcgacgac gtccaggccc gcgggaccat
    19681 cggccgccgt gatcgcgtcg tatccgagat cctcgaccat ccgcgcgacg acatcgcgga
    19741 ccagcgcttc gtcgtcgatc accagcaccg tgcgtccgtg cgccgagcca ttcgtcgacg
    19801 cgcgtgtcgc agtacctgca tccgccggcc acagcacttg aaacttcgca ccggtgccag
    19861 gcgccgagat cacgcggatg ccgccgccgt gcgcgcgcac gatgccgagc accgcggcca
    19921 ggccgaggcc atggccccga ttcttggtcg tgaagaacgg ctcgaagaca tgacggcgcg
    19981 tgtcggcatc catgccgggg ccgtcatcct cgacctcgat cacgacgtgc gtgcccgcgg
    20041 gcgccgtgag gacgtcgtgc ggatgcggct cgccgctgat ctcttcgatc gccgcgcgaa
    20101 ccgcgatctc accgccgcgg ccctgcagaa cgtcacgcgc gttgttgatg aggttgagca
    20161 tcacctggcg gagctgcgcg ggatcgccgc gcacggcgag cttggtcggg atctcgacgg
    20221 tgatgcgcac ctcgctcgga atgctgggcg agatgatccg caacagttcc tcgacgacga
    20281 gcgccggctc gacgcggatc gtccctgcgc cgccacgtcc ggcatacgcg agcaactgct
    20341 cggtgagctc ggcggcacgc agcccggcag tgcggatgtt ctcgagcgtg ctgcgtgcgt
    20401 tcttgccttc ggcttgctcg cgcatcgcga tgtcggcatt gccgagcacg gcgacgagca
    20461 ggttgttgaa gtcgtgcgcg aggccaccgg cgagcacgcc gagactctcg gcgcgttgcg
    20521 cgtcgaccat gcgccgctcg agcgcgcgcc agaccgtaac gtcgaggcac gtaccgatcg
    20581 cgccgatgat ctcgccggtg gcgttgcgat acggcgcgat caccgtcgcg agcaccttcg
    20641 cgcgatactc tgcttcgtac gtgacggtct cgccgttgca cgcgcgcgtg tgcgtcgcga
    20701 ccgtgtcgtt cgtcgatggg tcatcggcga tcgcgtcctg gaggcgccga ccgacgtagc
    20761 gattctggtc atagccgagc acctccatga tcgcgccgcc ggtgcgcagc aaacgcaggt
    20821 ccctatcgac gatccagtag atcgcgggca tctgccgcaa cacgaggtcg agaatcgacg
    20881 cggtcgtttc gcgttgctcg cgcgcctggc gaagctccgc ctccaacgcc gcgatccgtc
    20941 cctctagctc cgtctctctc gacactggca cgcactataa cagcagcgcg tcctcgatct
    21001 cctcgaatgc gccagcccgc cagcgggcga gtgggcttcg ccccttcaat agattttcgg
    21061 tctcggccag cacggcgccc agatcgacga gccagcagta cggatcgtag ggcgaccgca
    21121 tgcccacttc ctcgggaagc tcgacgggcg gcatccacgc cgccattttg tgggtcgcgt
    21181 tcgcgagcag gccgcgcagc gcaacatttg cgaggcggct gccgagcaag cggtcgaatg
    21241 cagatggacg accgccgcgc acgacgtggc cgagcaccgt cacgcgggtc tcgatcgtgc
    21301 tcggttccgc gtcgggatgg cgctcgcgca acctcgcgtc gacctccgtc ttgagcttgt
    21361 cgacctgcat ctgcacgcct tcggccttga tgacgatcac gcgacgcgcg cgccgttgcc
    21421 gagtgcgaat cgcgacgact gtctcgacaa tctggtcgac gatttc
//
LOCUS       DASUFX010000084         9708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_568961,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000084 DASUFX010000000
VERSION     DASUFX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568961"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..781
                     /locus_tag="VFV99_03295"
     CDS             440..781
                     /locus_tag="VFV99_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5058354.1"
                     /translation="MATSDLFWDQIVAIEPAGEAEVYDLTVPGPASWIANSSIISHNS
                     GAIEQDADVIMFIYRDVVYNKDCENPHVAEVILGKNRHGATGTVETHFEGRFTRFENL
                     AHRSDGPPRGD"
     gene            complement(783..1694)
                     /locus_tag="VFV99_03300"
     CDS             complement(783..1694)
                     /locus_tag="VFV99_03300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5058355.1"
                     /translation="MGNALEHWPLFFAVDALRYAIPASLGFAVFWLWKWDALSHRRIQ
                     KRRPSRNAFRREILYSISTAVIFSGVGVATFALVHAGVLHMYFRVDDYGWPWFFASIV
                     LAILIHDTYFYWTHRAIHHPWLFKRVHRVHHLSTSPSPWAAYAFAPPEALINALVFPL
                     ILLVVPMHELAGFVFLIYMITMNVIGHLGIELYPRGFTTSRWTRWYSTSTHHNLHHRD
                     FHGNYGLYFTWWDRMMRTQHEAYQQTFAQVTSRSEETDVQTFAQVTSDSEETEPADTI
                     VTGVFVRVHHDDGRDDAEGSHYVRKSA"
     gene            1837..3261
                     /locus_tag="VFV99_03305"
     CDS             1837..3261
                     /locus_tag="VFV99_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5058356.1"
                     /translation="MGLRTLVVVGLGAGLVLAWSPARADAPPPIADAIKQAAAAHKPL
                     VLEFGAQWCGPCKAFEKQVLPKSDVVSALTAVVFVRYDVDENVGAEAAAKYDVHSYPT
                     FIVVDRQGVVIARESGFLSAKPFIALIEKAKTEAVDEAEMRARVKEKPDDIAMQFVLA
                     SWLASHKRPLDAAPIYERISTHKLATKQQRVTATASLVRIRRVDQWKKHLVAEKAALI
                     RAAPEQAADDDILLAAMGSSIPIDERRALLKAMIAAEVDTARVNNLAYVGLAAGLTDE
                     ALVAAQRAVKEERIDQHLDTLAECFHIRGDRKAALATIDEAIGWARYNEILPTLQRNR
                     ARFESGGGDSPEVLQLRARTEDLWSRMEQVDQVAERVATPDDLEARRKVQADMTSFYR
                     AQSELSKKVAKNCAQDANGNEEAIARVELDSQGTIKSIVLLLEPTATPTLRTCLTQQL
                     TGQTLVADPKRTKTRLTIAFKGRE"
     gene            3428..3910
                     /locus_tag="VFV99_03310"
     CDS             3428..3910
                     /locus_tag="VFV99_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058357.1"
                     /translation="MRLDYGFADATVGIDNSPWNPSLRFEARTGDGYWQASVEHGRGA
                     CIDFDIDDPAYNNMPWRDSSSADHVLVDRIMRLGGDEAWLQANAVSTTSAIAFRRELQ
                     FALSECLLDARGVELADPPDGLCNFDAEDRCPDEPSADSDEGCPDPAPSPTQSATPAR
                     "
     gene            3964..5517
                     /locus_tag="VFV99_03315"
     CDS             3964..5517
                     /locus_tag="VFV99_03315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058358.1"
                     /translation="MPNPTPGTILGAYRVEGQLGAGSMGEVFKGVDTGLNRKVAIKIL
                     SEKHRDSPELRQRFVREGRAVAAISHPNVVQVFATGTFDDRPYIAMELLDGTDLGSIV
                     EKNGPLSSAEAAHAILDAAQGLAAASKAGLIHRDVKPSNLVRLSSNEVKVTDFGLAKP
                     VDPGSEPALTAMGVVVGTPDYIAPEQARGETIDERVDIYALGGTMYFLLTGIPPFRTG
                     KPAEDKYLKVVARHLRNPAPDAATTNPSVDRELANLTKRMMAKKPGERPSYQELIEGL
                     TTLLARIDPGSVPQLIATSRERSRPFKIPPDAGPGASLPEGGATLSDGELAAVPRAGL
                     PSWLVVFSLLCVALFVAGLVVYLQRGGDEPASPTPGSGSTAIAGSAAVVTPDAPPAPK
                     VPPAGMILVKLPDGNQLYVDAQPVSAERFRKLFADHKQPGKPDDAVVDITYDEARSFA
                     QSNEGRLLRAAEWTAASTTPGFISIDKMYEWVESPKEARQVVQHGKSQGRPDKAQKDV
                     TFRVARDIR"
     gene            5544..6458
                     /gene="deoC"
                     /locus_tag="VFV99_03320"
     CDS             5544..6458
                     /gene="deoC"
                     /locus_tag="VFV99_03320"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX5058359.1"
                     /translation="MSDVDRLVDLITKRVQERLNGQPQVKLNVLPRDRGECNDDQAPG
                     EDCTECGSCVVRRPWSVRAMEGAGAMRVGAAAGVGEVPADLGKLIDHTLLKPEATRDE
                     VVKLCEEARKHRFASVCVNTTWVPLCKSLLAGTDVMVCAVVGFPLGAMSPTAKAYEAR
                     EAVRQGAREIDMVINIGALKSRDYETVFEDICRVVKASGPAGVKVILETSALTQEEKI
                     IAISLSKLGGAAFVKTSTGFGKGGATVEDVQLMRQLVGTELGVKASGGVRTAEDAIKM
                     AQAGANRIGASASVAIVTGKTDAKAKGY"
     gene            6474..7844
                     /locus_tag="VFV99_03325"
     CDS             6474..7844
                     /locus_tag="VFV99_03325"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX5058360.1"
                     /translation="MLPVQLIRKKRDGGALDDDEIRAFIAGVTDGTIPDYQQAAMLMA
                     VFFEGLDARELATWADAMTRSGDVIDTSRVKAPKIDKHSTGGVGDKISIPLAPAVAAC
                     GVAVPMVSGRGLGHTGGTLDKLESIPGFRVDLSVDRFVDLVDEIGVCLIGQTERIAPA
                     DKRLYALRDVTATVESLPLIASSIMSKKLAEGIDGLVLDCKVGRGAFMKTVENARALC
                     ELIRTIGHGNGKRVTCVLTDMEAPIGRTIGNALEIRESIEVLRGGGPVDTRELTYVLG
                     AEMLMLAGVAKDPEDGRARIEKVLGDGSALEVFRKVVEAQGGDPRVCDSPGSVLPKPT
                     QRAELSLPPGRIAAIDSEALGIAALVLGAGRRKKEDSIDPAAGLVIDAYLGEVIEPDA
                     PPQIMLHHSLSPEDGRVAEARAMIFSAFVIEPVDHPAPPVRAEGASQAGPLGSVTRSR
                     ILEVIR"
     gene            7841..8704
                     /locus_tag="VFV99_03330"
     CDS             7841..8704
                     /locus_tag="VFV99_03330"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX5058361.1"
                     /translation="MSTKRVIETLYDRVNTAATSIKDRTGFAPQVGLILGSGLGAYGD
                     KLANAKKIPYADIPYFPQSHVVGHKGTLALGERTGVRCVAMQGRVHMYEGHSAATVAF
                     PARVLIALGAKVLIITNAAGGLNPSWSPGTLMLIRDHIDMLRDHALRGPNDERLGPRF
                     PDMTKAYAPELRAIAKEAAAGAKITMQEGVYVAMPGPTYETPAEVQMLQRIGADATGM
                     STVPEVVVARHMGARVIGISCITNQAAGISGQELSHDEVTETANRVRATFETLLDAIL
                     AKLVARGELGR"
     gene            8701..9099
                     /gene="cdd"
                     /locus_tag="VFV99_03335"
     CDS             8701..9099
                     /gene="cdd"
                     /locus_tag="VFV99_03335"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX5058362.1"
                     /translation="MSDKELEDRARAVQPRAHAPYSKFKVGAAVRVDGKVFEGVNVEN
                     ASYPLGVCAERNAIAAAVLAGAHHLERVAVYTDASPPSSPCGGCRQVLREFADDPTRV
                     TIIAINGKGERREWTLDQLLPDSFSGRELP"
     gene            9096..>9708
                     /locus_tag="VFV99_03340"
     CDS             9096..>9708
                     /locus_tag="VFV99_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5058363.1"
                     /translation="MSEVVFRGGTILTIDPEHRVLAGDVATLDGAIVHVGGSYTPQTS
                     DYTILDCAGCVVMPGLVQAHVHTVQTLARGRADDLELLDWLRNVIWPYEAALDGEAAS
                     AAGELACAELLLGGTTAILDMATVHHTDAIFSAAERAGIRATIGKAMMDEPDPQIPPG
                     LRETTKHSLDESATLIKRWHGAANDRLRYAYAPRFVLSCTDELL"
BASE COUNT         1767 a         3212 c         3228 g         1501 t
ORIGIN      
        1 atcccgcgtt tacttcgcga caaatagcaa aggcctcgct accgacgtcg cggcattgct
       61 gctccggctg gatgtcgtcg cccgcaccca tgaggtagcg cagggaacgt atcgctcgca
      121 gtttccagtc gacatccgtg gcagcgaaca gcagcgtcgc ttcctagagc tcgtgggagc
      181 ccatgggccg cgcgtcccac aagccgaaac gttgcgcgag tacctccacg atcgcgttgc
      241 caacacgaac gtcgacacac ttcctgagag tcttgtgcat gaacgcgtcg agctcctgat
      301 gaaggagcga ggcatgactc gccatgacgt cgccaaggca caaggcgtcg cgtccggaag
      361 cggaatgttc tcgtacgcgc cttcgcgtag gacagtctcg cgctacgccg aactattcga
      421 cgatgctgag ttgagcgcga tggcaacgag cgatctgttt tgggatcaga tcgtagcgat
      481 cgagccggca ggggaagcag aggtctacga ccttaccgtt cctggacctg cctcctggat
      541 cgcaaattca tcaataattt cacataattc gggcgcgatc gagcaggatg cagacgtcat
      601 catgttcatc taccgcgacg tcgtctacaa caaggactgc gagaacccgc acgtcgccga
      661 agtcatcctc ggcaagaacc gccacggcgc gacgggcacg gtcgagacgc acttcgaggg
      721 ccgcttcacg cggttcgaga acctcgcgca ccgcagcgac ggcccgccgc gcggcgactg
      781 agctaggcgc tctttcgaac gtagtgcgag ccttcggcgt catcgcgccc gtcgtcgtgg
      841 tgcacgcgca cgaagacgcc agtcacgatc gtatcggcgg gttcggtttc ctccgagtcc
      901 gaggtcacct gagcgaacgt ctgcacatcc gtttcctctg agcgcgacgt cacctgcgcg
      961 aacgtctgct ggtaggcctc gtgctgcgtg cgcatcatgc gatcccacca tgtgaagtag
     1021 aggccgtaat tgccgtggaa gtcgcggtgg tggaggttgt ggtgcgtgga tgtcgagtac
     1081 cagcgcgtcc accggctcgt cgtgaacccg cgcggataga gctcgatgcc gagatgaccg
     1141 atcacgttca tcgtgatcat gtagatgagg aagacaaacc cggcgagctc gtgcatcggc
     1201 acgacgagca ggatcagcgg gaacacgagc gcgttgatca gtgcctcggg cggcgcgaac
     1261 gcgtatgcag cccacggcga cgggctcgtc gacaggtgat ggacgcgatg cacgcgcttg
     1321 aacagccacg ggtgatgaat cgcgcgatgc gtccagtaga agtacgtgtc gtggatcagg
     1381 atcgcgagca cgatactcgc gaagaaccac ggccagccgt agtcgtcgac gcggaagtac
     1441 atgtgcagca cgcccgcgtg cacgagcgca aacgttgcga cgccgacgcc tgagaagatc
     1501 accgccgtcg agatcgagta gagaatctcg cgccggaatg cgttgcggct cggccgccgc
     1561 ttctggatgc ggcgatgcga gagcgcgtcc cacttccaga gccagaacac ggcaaacccg
     1621 agcgatgccg ggatcgcgta gcgaagcgca tcgaccgcga agaacagagg ccagtgttcc
     1681 agagcattcc ccattgcagt cggccttctt tcacacagcg cgccacatcg ccgcgtcgcg
     1741 gaaacaagtg gttggcgcgc agccgcaatg tccctgcgac tgtcgcagct actgccgctg
     1801 tcgcagacgg gtggccgaaa gcgtagggtc ttgaacatgg gtctacgaac gttggtcgtc
     1861 gtcggactgg gcgccgggtt ggtcctcgcc tggtcgccag cgcgtgcaga tgcgccaccg
     1921 ccgatcgcgg acgcgatcaa gcaggccgcc gctgcgcaca agccgctcgt gctcgagttc
     1981 ggcgcgcagt ggtgcgggcc gtgcaaggcg ttcgagaagc aggtcctgcc gaaatccgac
     2041 gtcgtctctg cgctcacggc cgtggtgttc gttcgttatg acgtcgacga gaatgtcggc
     2101 gcagaagccg ccgcgaagta cgacgtccat tcgtacccga cgttcatcgt ggtcgatcgc
     2161 caaggtgtgg tgatcgcgcg cgagagcggc tttctgagtg cgaagccgtt catcgctctg
     2221 atcgagaaag ccaagaccga agccgtcgac gaggccgaga tgagggcccg ggtcaaagag
     2281 aagcctgacg acatcgcgat gcagttcgtg ctcgcgagct ggcttgcatc gcacaaacgg
     2341 cctctcgacg cggcgccgat ctacgaacgc atctcgacgc acaaactcgc gaccaagcag
     2401 caacgcgtaa ctgccacggc gtcgctcgta cgcatccgcc gtgtcgatca atggaagaag
     2461 cacctggtgg cggagaaggc cgcgctgatt cgcgcagcac ccgagcaagc ggcggatgac
     2521 gacatcctgc tcgcggcgat gggctcgagt attccgatcg acgaacggcg tgctctgctc
     2581 aaggcgatga tcgcggcgga agtcgacacg gcacgggtga acaaccttgc ctatgtcggc
     2641 ctcgccgccg ggctcaccga cgaggccctc gtggctgcgc aacgcgccgt taaagaggag
     2701 cgaatcgacc agcatctcga cacgctggcc gagtgctttc acatccgcgg cgatcgcaag
     2761 gcggcacttg caacgatcga cgaagcgatc ggttgggcgc gctacaacga gatcctgccg
     2821 acgctgcagc gaaatcgtgc acgtttcgag tccggcggcg gcgactcccc cgaggtccta
     2881 cagctgcgcg cgcggacgga ggatttgtgg tcacggatgg agcaggtcga tcaggtcgcc
     2941 gagcgcgtgg cgacgccgga tgacctcgaa gcacgtcgaa aagtgcaggc cgacatgacc
     3001 agcttctatc gggcccagtc cgagctctcg aaaaaggtcg cgaagaactg cgcccaggat
     3061 gcgaatggca acgaggaggc gatcgcacgc gtcgagctcg actcgcaggg caccatcaaa
     3121 tcgatcgtgt tgctgctcga accgaccgcg acgccgacgc tgcgtacatg cctgacccag
     3181 cagctgaccg gtcagacgct ggtcgccgat ccaaagcgaa cgaagacgcg gctgaccatc
     3241 gctttcaagg gccgcgagta accacggcgg tcgcgcacgg cactgaccgg tatgcgcgcg
     3301 gtctggctcg tcaccctcgc tgcctgcggt cacccacccg ccaaggcgcc cgcgccgcgc
     3361 gtcgagctat cgaccacgca gtggaccgcg ctgccgcgat cgcatcgagc ttgcgcgact
     3421 cgcgcccgtg cgcctcgatt acgggtttgc cgatgccacg gtcgggatcg acaactcgcc
     3481 ctggaatccg tcgctgcgct tcgaggcccg cactggcgac ggctactggc aggcgagcgt
     3541 cgagcacggc cgtggcgcct gcatcgactt cgacatcgac gaccccgcct acaacaacat
     3601 gccgtggcgc gacagcagct ccgcggacca cgtgctcgtc gatcggatca tgcgcctcgg
     3661 cggcgacgag gcgtggctgc aggcgaacgc ggtctcgacg acgagcgcga tcgcgtttcg
     3721 ccgcgagctg cagttcgcgc tcagcgagtg cctgctcgat gcgcgcggcg tggagcttgc
     3781 cgatccaccg gacgggctgt gcaatttcga cgcggaggat cgctgccccg atgagccgag
     3841 cgcggacagc gacgagggtt gcccggatcc tgctccgtcg cccacgcagt ctgcgacgcc
     3901 cgctcgctag aaattcgcgg ccgtgtcgcg aagatagcgg ccaagccgcg ttaagatggc
     3961 gccgtgccga accctacgcc ggggaccatc cttggtgcat atcgcgtcga gggacagctc
     4021 ggcgcgggct cgatgggcga ggtcttcaag ggcgtggaca cggggctcaa tcgcaaggtc
     4081 gcgatcaaga tcctctccga gaagcaccgc gatagccccg agctgcgcca gcgcttcgtg
     4141 cgcgagggac gcgcggtcgc ggcgatcagt cacccgaacg tcgtgcaggt gttcgcgacc
     4201 ggcacgttcg acgaccggcc gtacatcgcg atggagctcc tcgacggcac cgacctcgga
     4261 tcgatcgtcg agaagaacgg cccgctgagc tccgcggaag cggcgcacgc gatcctcgac
     4321 gccgcgcagg gactcgccgc cgcatcgaag gctggcctga tccaccgcga cgtcaagccg
     4381 tcgaacctgg tgcgactgtc gagcaacgaa gtgaaagtga ccgacttcgg cctcgcaaag
     4441 cccgtcgatc cgggcagcga accggcgctc actgcgatgg gcgttgtcgt cggcacgccc
     4501 gattacatcg cgcccgagca ggcacgcggc gagacgatcg acgagcgcgt cgatatctac
     4561 gcacttggcg gcacgatgta cttcctgctc actggcatcc cgccgtttcg caccggcaag
     4621 cccgccgagg acaagtacct caaggtcgtc gcgcgccacc tgcgcaaccc cgcacccgac
     4681 gcggcgacga cgaatccgtc ggtcgatcgc gagctcgcga acctcacgaa gcggatgatg
     4741 gcgaagaagc ccggcgagcg gccgtcgtac caggagctca tcgaagggct cacgacgctg
     4801 ctcgcgcgca tcgatcccgg ctcggtgccg cagctgatcg cgacatcgcg cgagcgatcg
     4861 cgaccgttca agattccgcc cgacgcgggg ccaggcgcct cgcttcccga aggcggcgcg
     4921 acgctcagcg acggcgagct cgcggcagtc ccgcgtgcgg ggctcccgtc ctggctcgtc
     4981 gtgttctcgc tgctctgtgt cgcgttgttc gtcgccggcc tcgtcgtcta cctgcagcgg
     5041 ggcggcgacg agcctgcctc gcccacgccc ggcagcggct cgacagcaat cgcgggtagc
     5101 gccgcggtcg tgacgccgga tgcaccgccg gcgccgaagg tcccgcccgc cggcatgatt
     5161 ttggtgaagc tgcccgacgg caaccagctc tacgtcgacg cccagccagt cagcgccgag
     5221 cgcttccgca aactcttcgc cgaccacaag cagcccggta agcccgacga cgcggtcgtc
     5281 gacatcacgt acgacgaggc gcgatcgttc gcgcaatcca atgagggccg cctcctgcgc
     5341 gcggccgaat ggaccgccgc ctcgacgaca ccgggcttca tctcgatcga caagatgtac
     5401 gaatgggtcg aatcgcccaa agaggcccgc caggtcgtcc agcacgggaa atcgcagggc
     5461 cggcccgaca aggcacagaa ggacgtcacg ttccgggtag cgcgcgacat ccgctgagat
     5521 tccctgggct atagtgcgcc ggcatgtcgg atgtagaccg cctcgtcgac ctgatcacca
     5581 agcgggttca ggagcgcctg aacggccagc cgcaggtgaa gctcaacgtg ctgccgcgcg
     5641 atcgcggcga gtgcaatgac gaccaggcgc cgggcgaaga ctgcaccgag tgcggctcgt
     5701 gtgtggtgcg ccggccgtgg tcggtgcgcg cgatggaagg tgccggcgcg atgcgcgtcg
     5761 gtgcggccgc aggcgtcggc gaggtccccg cggatctcgg gaaactgatc gaccacacgc
     5821 tgctcaagcc cgaggcgacg cgcgacgagg tcgtgaagct ctgcgaagaa gcgcgcaagc
     5881 atcgcttcgc gagcgtgtgt gtgaacacga cgtgggtgcc gctctgcaag tctctgctcg
     5941 cgggcacgga cgtcatggtg tgcgcggtcg tcggttttcc gctcggtgcg atgtcgccga
     6001 cggcgaaggc ctacgaggcg cgcgaggccg tacgtcaggg cgcgcgagag atcgacatgg
     6061 tgatcaacat cggcgcgctc aagtcgcgcg attacgagac cgtgttcgag gacatctgcc
     6121 gcgtcgtgaa ggcgtcgggt cccgccggcg tgaaagtgat cctcgagacc agcgcgctca
     6181 cgcaggaaga aaagatcatc gcgatctcgc tctcgaagct cggcggcgct gcgttcgtca
     6241 agacgtcgac tggcttcggc aaaggcggcg cgaccgtcga ggacgtccag ctgatgcgtc
     6301 agctcgtcgg taccgagctc ggcgtcaaag catccggcgg cgtgcgcacg gccgaagatg
     6361 cgatcaagat ggcgcaggcg ggcgcgaacc gcatcggcgc gagcgcgtcg gtcgcgatcg
     6421 tgacgggcaa gacggacgcg aaggcgaagg gctactagtc cgctgacagt ccgatgctgc
     6481 ccgttcaatt gatccgcaag aagcgcgacg gcggcgcgct cgatgacgac gagattcgcg
     6541 cgttcatcgc cggcgtcacc gacggcacca tcccggacta ccagcaagcc gcgatgctga
     6601 tggccgtgtt cttcgaaggc ctcgacgcgc gcgagctcgc gacctgggcc gacgcgatga
     6661 cccgctcggg cgacgtgatc gacacgtcgc gcgtgaaggc gccgaagatc gacaagcact
     6721 cgacaggcgg cgtcggcgac aagatctcga tcccactcgc gccggcagtt gctgcgtgcg
     6781 gcgtcgcggt gccaatggtg tcgggtcgcg gactcggtca caccggcggc acactcgaca
     6841 agctcgagtc gattcccggc ttccgcgtcg atctcagcgt cgatcggttc gtcgatctcg
     6901 tcgacgagat cggcgtgtgc ttgatcggcc agaccgagcg catcgcgcct gccgacaaac
     6961 ggctctacgc gctgcgcgac gtcacagcaa cggtcgagtc gctgccgctg atcgcgtcgt
     7021 cgatcatgag caagaagctc gcggagggca tcgacggcct ggtgctcgac tgcaaggtcg
     7081 gccgcggcgc gttcatgaag accgtcgaga acgcgcgcgc gctatgcgag ctcattcgca
     7141 cgatcggtca cggcaacggc aagcgcgtga cctgcgtcct caccgacatg gaagcaccga
     7201 tcgggcgcac gatcggcaac gcgctcgaga ttcgcgagtc gatcgaggtg ctgcgtggcg
     7261 gcggcccggt cgatacgcgc gagctgacct acgtgctcgg cgccgagatg ctgatgctgg
     7321 ccggcgtcgc gaaagatccc gaggacggcc gcgcgcgcat cgagaaggtg ctcggcgacg
     7381 gctccgcact cgaagtgttc cgcaaggtgg tcgaagccca gggcggcgac ccgcgcgtct
     7441 gcgactcgcc cggcagcgtg ctgccgaaac cgacccagcg cgcagagctg tcgctaccgc
     7501 ctggccgcat cgccgcgatc gacagcgagg cgctcggcat cgccgcgctc gtactgggcg
     7561 caggccgtcg caagaaggaa gactcgatcg atcccgccgc gggcctcgtc atcgacgctt
     7621 atttaggtga ggtcatcgag cctgacgcac cgccgcaaat catgctgcat cacagccttt
     7681 cgcccgaaga tggcagggtc gccgaggcgc gtgcgatgat tttttctgcc ttcgtgatcg
     7741 agcccgttga ccaccccgcc ccaccggtcc gcgccgaggg cgccagccag gctggccccc
     7801 tcggatcggt gacacggagc cgaatcctcg aggtgatccg gtgagcacca agcgcgtcat
     7861 cgagacgctc tacgatcgcg tgaataccgc ggcgacctcg atcaaggatc gcaccgggtt
     7921 cgcgccgcag gtcggcttga tcctcggctc gggcctcggc gcctacggcg acaagctcgc
     7981 gaacgcgaaa aagattccgt acgcggatat cccgtacttc ccgcagtcgc acgtcgtcgg
     8041 ccacaagggc acgctcgcgc tcggtgagcg caccggcgtc cgctgcgtcg cgatgcaggg
     8101 ccgcgtgcac atgtacgaag gccacagcgc ggcgaccgtc gcgttcccgg cacgtgtgct
     8161 catcgcgctc ggcgcgaagg tcctcatcat cacgaacgcg gccggtggtc tcaatcctag
     8221 ctggtcgccc ggcacgctga tgctgatccg cgatcacatc gacatgctgc gcgaccacgc
     8281 gctgcgcggt cccaacgacg agcgactcgg cccgcgcttc cccgacatga cgaaggcgta
     8341 cgcgccggag ctgcgcgcga tcgcgaagga agctgccgcc ggcgcgaaga tcacgatgca
     8401 agaaggcgtg tacgtcgcga tgcccggccc gacctacgaa acgcccgccg aagtgcagat
     8461 gttgcaacgg atcggcgccg acgcgaccgg catgtcgacc gtgcccgaag tcgtcgtcgc
     8521 gcgacacatg ggcgcgcgcg tgatcggcat ctcgtgcatc acgaatcagg cagccggtat
     8581 cagcggccag gagctatcgc acgacgaggt caccgagaca gcgaaccgcg tgcgcgcgac
     8641 gttcgagacg ctgctcgacg cgattctcgc caagctcgtc gctcgcggag agctcggacg
     8701 atgagcgaca aagagctcga ggatcgcgcg cgtgcggtgc aaccgcgggc gcatgcgccg
     8761 tactcgaagt tcaaggtcgg cgcagccgtg cgcgtcgacg gcaaggtgtt cgagggcgtc
     8821 aacgtcgaga acgcgtcgta tccgctcggc gtgtgcgcgg aacgcaacgc gatcgcagcc
     8881 gccgtactcg ccggtgcgca tcacctcgag cgcgttgcgg tgtacaccga cgcatcgccg
     8941 ccgtcgtcgc cgtgcggtgg ttgccgccag gtgttgcgcg aattcgccga cgatccgacg
     9001 cgcgtcacca tcatcgcgat caacggcaag ggcgagcgcc gcgagtggac gctcgatcag
     9061 ctcctgcccg acagcttctc ggggcgcgag ctgccatgag cgaggtcgtc ttccgcggcg
     9121 ggacgatcct gacgatcgat cccgagcaca gagtgctggc cggcgacgtc gcgacactcg
     9181 acggcgcgat cgtgcacgtc ggcggttcgt acacgccgca gacgagcgac tacacgatcc
     9241 tcgactgcgc aggctgcgtc gtgatgcccg gcctcgtgca ggcacacgtc cacaccgtgc
     9301 agacgctcgc gcgcggccgc gccgatgatc tcgagctact cgattggttg cgcaacgtga
     9361 tctggccgta cgaggccgcc ctcgacggcg aggccgcgag tgccgcgggc gagctcgcat
     9421 gcgcggagct cctcctcggc ggcacgacgg cgatcctcga catggcgacg gtgcatcaca
     9481 ccgacgcgat tttctcggcc gccgagcgtg ccggcattcg cgcgacgatc ggcaaggcga
     9541 tgatggacga gccggatccg caaatcccac cgggcttgcg cgagacgacg aaacactccc
     9601 tcgacgaatc ggcgacgctc atcaagcgct ggcacggagc cgcgaacgat cgcctgcgct
     9661 acgcgtacgc gccgcggttc gtgctgtcat gtaccgacga gctgcttc
//
LOCUS       DASUFX010000085         6180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1124495, whole genome shotgun sequence.
ACCESSION   DASUFX010000085 DASUFX010000000
VERSION     DASUFX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124495"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VFV99_03345"
     CDS             <1..1638
                     /locus_tag="VFV99_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5058364.1"
                     /translation="PAVRAATGAADHRAGVAPMTSASFWSEPRGVRSWLMTHDHKRIG
                     ILYLGSVILFLMLGGVFALVLRLSLVKPDNALVDAHTYDELFTQHGIVMVWLFMIPSI
                     PSALGNFLVPLMIGARDVAFPRINLASYWIFVAGAIIVLAALVAGGVDTGWTFYAPYS
                     TSTRTAVVLAAVGVFVTGISSILTGLNFIATVHTMRVQGLRWRDMPLFVWTIYGTSII
                     LVLATPVLGLALLLVGADHVWHLGLFDPSTGGDPILFEHIFWFYSHPAVYIMILPAMG
                     VVSEIVCTFSHRRPSWYGAIVVATIGIAIVGFLTWGHHMFVAGMSTFDVGMFGILSMM
                     VAIFSAIKVFTWTATMYGGSITIATPILYVFTFIFLFVFGGMSGVAVATSSLDVHWHD
                     TYFIVAHFHFIMVGGTMTGFLAALHYWFPKITGKRYSEQWARLGCLTVSFGFILTFTP
                     QFLLGNAGMPRRYATYPEQYQPLHAVSTAGACLLAAGMVITLGYFVIAIIRGKPAGAN
                     PWRSKSFEWRSPSPPPPENFDELPLWSDTPYSYEEPS"
     gene            1635..2237
                     /locus_tag="VFV99_03350"
     CDS             1635..2237
                     /locus_tag="VFV99_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5058365.1"
                     /translation="MNVTNEERRYRIAVWIVIASEALLFSGLFTLYASYRHEYASAFD
                     AGVREGVHWIGGINTLILLTSSFAIAWSIHLIRSDRGRGAIWSLAAVLGLGLAFLVLK
                     GIEWTKHIHDGIVPGIAYAGPDHGPGTSLYFTLYYGMTGLHALHVIAGLGLVTWVFTR
                     AYRRRYAPERHLSLELVALYWHFVDAIWVFLWPMFYLMHG"
     gene            2242..2505
                     /locus_tag="VFV99_03355"
     CDS             2242..2505
                     /locus_tag="VFV99_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX5058366.1"
                     /translation="MHAPFTGRALVLTFIALIALAGISWVAATLGTGTGVALSIASLK
                     ALAIALVFMELGRAHAVDRLIPVVAVGFVLLLCLGAVADVAFR"
     gene            complement(2509..5466)
                     /locus_tag="VFV99_03360"
     CDS             complement(2509..5466)
                     /locus_tag="VFV99_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058367.1"
                     /translation="MAVRVRRAAWIGCLVALTSTAHAYVPKSGPQVVADSLTALPAAN
                     ITKPLRLQPQLRYNEQTPPLPWSQFIAQRGSKWHASWDVATGVPNRIWGAGMWAPGTV
                     RNADAAERFARQVLADHLGLLAPGAALADFELASNVSDGTMRTIGFFQRVSGRRVIGG
                     QISFRFKNDRLFVIGSEALPNVAASMPMVKQPMAKLPPATMRARAIDRLRGQLALPNA
                     PVDALGDEVIVPLITDDAILGYRIARPTTIDGGADGRYLAYVDITTGEPLAVRQLNAY
                     ASATVLYNTVDRYKLAGRGRASAPAPRAYLKINGTDVTSTMAGGVMWSPDSPTSVQTS
                     VTGDLVTVVNKWDADENGMADAAVTGSLAVSPGDTLTWDTSAVEEDDAQVNAYIATGL
                     AKEYVRNNIDAAMPTLDEQMTVNVNIDQACNAFFDGKALNFLHSSSSCQNTALIQDVV
                     FHEYGHRLHTAEILQGVGDFDSAMSEGASDFLAASMTGDSGMGRGFQYLDAPLRELDP
                     PDHEWSWPLDIGEAHHTGQIYGGIFWDLRKQLIADLGMGPGSALTNKLYLGTLRRAVN
                     IPTSLIEALAEDDDDGNLDNGTPHECAIRAAFGRHGLRTATGTVVAPGHLEENALAIG
                     VHIEVTGLSDRCTGDEVESAQLEYSSFKLTPSSGKVDATPAGTNTFFAQLPLAPQDSV
                     YYKAVVHFVDGSTMELADNRADPWYQLYSGPTVKLYCTDFESTDPFADGWTAGTDNNP
                     DHFWQWGVPTSGAYDPHAAFSGQKILALGLNGNYMPKQHTWAKTPVINIGQYSDVRLH
                     YRRWLTVEDNHFDQATINANGKKAWNNFDSAQGDSSAVHHVDREWRFQDVPLSGYFSG
                     HNVQVSWDLTSDEGLELGGWQLDDVCIVANPYSICGDGVRTPTEQCDNGAANQDKPDV
                     CRTDCRLPTCGDSIVDMGEECDEGSDGTMRCTDKCTIIDEGGGCCSANGGEVGSLSLA
                     GLVGLLLLRRRRR"
     gene            complement(5561..>6180)
                     /locus_tag="VFV99_03365"
     CDS             complement(5561..>6180)
                     /locus_tag="VFV99_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX5058368.1"
                     /translation="ASSHSDSPYARLAKTHSLPCSRVDPQPLRELSVRHGRSGGTPNA
                     PRGCTMSRVLVAYACYIGRARAIAEDLAGRLRARGHEVDVANARAGRLPPPQDYDAVI
                     LGSQVEHRHPCREIREYARIYGHELDHMPTGFFSVSLAASVPFANPDPGRHVAQFVAD
                     VGWVPDHVASFGTFGTPQSWKLADLVTVSLAEHPSCELSPSYGPV"
BASE COUNT          933 a         2063 c         2007 g         1177 t
ORIGIN      
        1 cccgccgtgc gtgcagcaac cggtgccgcc gatcatcgag ccggagttgc gccgatgacg
       61 tccgctagct tctggagtga gccgcgtggg gtgcggtcgt ggctgatgac ccacgatcac
      121 aagcgcatcg gcattctcta cctcggctcg gtaatcctgt tcctgatgct cggcggggtg
      181 ttcgcgctcg tgctgcggct gtcgctcgtc aagcccgaca atgcgctcgt cgatgcccac
      241 acgtacgacg agctgttcac gcagcacggc atcgtcatgg tctggctgtt catgatcccg
      301 tcgatcccgt cggcgctcgg caatttcctc gtgccgttga tgatcggcgc gcgcgacgtc
      361 gcattcccgc gcatcaatct ggcgagctac tggatcttcg tcgccggcgc gatcatcgtg
      421 ctggccgcgc tcgttgccgg cggcgtcgac accgggtgga cgttctacgc gccgtacagc
      481 acgagtacgc gcactgcggt ggtgctagct gcggtcggcg tgttcgtcac cggcatctcg
      541 tcgatcctca ccggtctcaa cttcatcgcg accgttcaca cgatgcgggt ccagggcctg
      601 cgttggcgcg acatgccgct gttcgtgtgg acgatctacg gcacgtcgat catcctcgtg
      661 ctcgcgacgc ccgtgctcgg gctcgcgctg ttgctcgtcg gcgccgacca cgtctggcat
      721 ctcggtctct tcgatccgag caccggcggc gatccgatcc tgttcgagca catcttctgg
      781 ttctactcgc accccgccgt ctacatcatg atcttgcctg cgatgggcgt ggtcagcgag
      841 atcgtgtgca cgttctccca ccgcagaccg tcgtggtacg gcgcaattgt cgtcgcgacg
      901 atcggcatcg cgatcgtcgg cttcctcacc tggggccacc acatgttcgt cgccggcatg
      961 tcgacattcg acgtcggcat gttcggcatc ctgtcgatga tggtcgcgat cttctcggcg
     1021 atcaaagtct tcacgtggac ggcgacgatg tacggcggct cgatcacgat cgcgacgccg
     1081 atcctctacg tgttcacgtt catcttcttg ttcgtgttcg gcggcatgtc gggcgtcgct
     1141 gtcgctacgt cgtcactcga cgtgcactgg cacgacacgt acttcatcgt cgcgcacttc
     1201 cacttcatca tggtcggcgg gacgatgacg gggttcctcg cggcactcca ctactggttc
     1261 ccgaagatca cgggcaagcg gtactcggaa cagtgggcgc ggctcggctg cctcacggtc
     1321 tcgttcggct tcatcctcac gttcacgccg cagttcctgc tcggcaacgc tggcatgccg
     1381 cgccgctacg cgacgtaccc ggagcagtac caaccgctgc acgcggtctc gacggcgggt
     1441 gcgtgtctgc ttgccgccgg catggtgatc acgctcggct atttcgtcat cgcgatcatc
     1501 aggggcaagc ctgcgggcgc gaacccgtgg cggtcgaaga gcttcgagtg gcgctcgccg
     1561 tcaccgccgc cgccggagaa cttcgacgag ctgccgctct ggagcgacac cccgtattcg
     1621 tacgaggagc cgtcgtgaac gtcaccaacg aggagcgccg ctatcggatc gcggtgtgga
     1681 tcgtgatcgc gagcgaggcg ctcctgttct ccggcctgtt cacgctctac gccagctacc
     1741 gccacgagta cgcgtccgca ttcgacgccg gcgtgcgcga gggcgtccac tggatcggcg
     1801 gcatcaacac gctgatcctc ctgacgtcga gctttgcgat cgcgtggtcg atccatctga
     1861 tccgaagcga tcgcgggcgc ggcgcgatct ggagccttgc cgcggtgctc gggctcgggc
     1921 tcgcgttcct cgtgctgaag ggcatcgagt ggaccaagca catccacgac ggcatcgtgc
     1981 ccggcattgc ctacgcaggc cccgatcacg gccccggtac gtcgctgtac ttcacgctct
     2041 actacggcat gaccgggctg cacgcgctgc atgtcatcgc cggtcttggc ctcgtcacct
     2101 gggtgttcac gcgcgcgtat cgccgccgct acgcccccga gcgccacctc tcgctcgagc
     2161 tcgtcgcgct gtactggcac ttcgtcgatg cgatctgggt gtttctgtgg ccgatgttct
     2221 acctcatgca cggatgacgc tatgcacgca ccttttaccg gccgcgctct cgtcctcacg
     2281 ttcatcgcgt tgatcgcgct tgccgggatc agctgggtgg cggcgacgct tggcaccggc
     2341 accggcgtcg cactctcgat cgcatcgctg aaggcgctcg ccatcgcgct cgtattcatg
     2401 gagctcgggc gcgcgcacgc ggtcgatcgc ctcatcccgg tcgtcgcggt cgggttcgtg
     2461 ctgctgctct gcctcggcgc ggtcgccgac gtcgcgttcc gttaggaact aacggcgccg
     2521 ccggcgcagt agcagcaagc cgaccagacc cgcgagcgag agcgaaccga cctcgccacc
     2581 gttcgccgag cagcaaccgc cgccctcgtc gatgatcgtg cacttgtcgg tgcatcgcat
     2641 cgtgccgtcg gagccttcgt cacactcctc acccatatcg acgatgctgt cgccgcacgt
     2701 cggcagccgg caatcggtgc gacacacgtc gggcttgtcc tggttcgccg caccgttgtc
     2761 gcactgctcg gtcggtgtcc gcacgccgtc gccgcagatc gagtacgggt tcgcgacgat
     2821 gcagacgtca tcgagctgcc agccgccgag ctcgaggcct tcgtcgctcg tgaggtccca
     2881 gctcacctgg acgttgtgac cactgaagta gcccgacagc gggacgtcct ggaatcgcca
     2941 ctcgcgatcg acatgatgga ccgcgctcga atcaccctgc gcgctgtcga agttgttcca
     3001 cgccttcttg ccgttcgcgt tgatcgttgc ctgatcgaag tggttgtctt cgacggtgag
     3061 ccaccggcgg tagtgcaaac gcacgtcgct atactggccg atgttaatca ccggcgtctt
     3121 cgcccacgtg tgctgcttcg gcatgtagtt gccgttgagc ccgagcgcga ggattttctg
     3181 gcccgagaac gccgcatgcg gatcgtaggc gccgctcgtc ggtacacccc actgccagaa
     3241 gtggtccggg ttgttgtcgg tgcccgcggt ccagccatcg gcaaacggat ccgtcgactc
     3301 gaagtcggtg cagtacagct tcaccgtcgg ccccgagtac agctggtacc aagggtcggc
     3361 tcggttgtcg gcaagctcca tcgtcgagcc atcgacgaaa tgaacgaccg ccttgtagta
     3421 gacggagtcc tgcggtgcga gcggcagctg ggcaaagaac gtgttcgtac cggctggtgt
     3481 cgcgtcgact ttgcccgacg atggcgtcaa cttgaacgag gaatactcga gctgcgcgct
     3541 ctcgacttcg tcgccggtgc agcgatcgga caggccggtg acttcgatgt ggacgccgat
     3601 cgcgagcgcg ttctcttcga ggtggcccgg tgcgaccacg gtgcccgtcg ccgtgcgcag
     3661 gccatggcgg ccgaacgcgg cgcgaatcgc gcactcgtgc ggcgtgccgt tatcgaggtt
     3721 gccgtcatcg tcgtcttcgg cgagcgcctc gatgaggctc gtcgggatgt tgaccgcgcg
     3781 gcgcagcgtg ccgaggtaga gcttgttcgt gagcgcgctg cccgggccca tgccgaggtc
     3841 ggcgatcagc tgcttgcgca gatcccagaa gatgccgccg tagatctggc ccgtgtggtg
     3901 ggcctcgccg atgtcgagcg gccaactcca ttcgtgatct ggtggatcga gctcgcgcag
     3961 cggcgcgtcg agatactgaa accctctccc cattcccgag tcccccgtca tgctcgcggc
     4021 gaggaagtcc gacgcacctt cgctcatcgc gctgtcgaag tcgccaacgc cctggagaat
     4081 ctcggccgtg tgcaggcggt ggccgtactc gtggaacacg acgtcctgga tgagcgcggt
     4141 gttctggcag ctcgagctcg agtgcaggaa gttgagcgct ttgccgtcga agaacgcgtt
     4201 gcatgcctgg tcgatgttga cgttgacggt catctgctcg tcgagcgtcg gcatcgcggc
     4261 gtcgatgttg ttgcgcacgt actccttcgc gagtcccgtc gcgatgtacg cgttcacctg
     4321 cgcgtcgtcc tcttcgaccg cgctcgtgtc ccaggtcagc gtgtcgcccg gtgacaccgc
     4381 gagcgagcca gtcacggctg cgtccgccat cccattttca tcggcgtccc acttgttgac
     4441 gacggtcacg aggtcgccgg tcaccgaggt ctggaccgac gtcgggctat cgggggacca
     4501 catcacgccg ccggccatcg tcgacgtcac gtcggtgccg ttgatcttga gataagcgcg
     4561 cggggccggc gcactcgcac gaccgcggcc ggcgagcttg tagcgatcga cggtgttgta
     4621 gagcaccgtc gcgctcgcat aggcgttgag ctggcgcacc gcgagtggct cgccggtcgt
     4681 gatgtcgaca tacgcgagat accggccgtc ggcaccgccg tcgatcgtcg tcggccgcgc
     4741 gatgcgatag ccgaggatcg cgtcgtcggt gatcagcggg acgatcactt cgtcgccgag
     4801 cgcatcgacc ggcgcattcg gcagtgcgag ctggccgcgc aacctgtcga tcgcacgtgc
     4861 gcgcatcgtc gccggcggca acttcgccat cggctgcttc accatcggca tcgaggccgc
     4921 gacgttcgga agcgcttcgg aaccgatcac gaacaagcga tcgttcttga agcggaagct
     4981 gatctggccg ccgatcacgc ggcgtcccga cacgcgctgg aagaacccga tcgtgcgcat
     5041 cgtgccgtcg ctgacgttcg acgcgagctc gaagtcggcg agcgcagcac cgggcgcgag
     5101 caggccgaga tggtccgcga gcacctggcg cgcgaaccgc tccgcggcgt ccgcgttgcg
     5161 caccgtgcca ggcgcccaca tgccggcgcc ccagatccgg ttgggcacgc cggtcgcgac
     5221 gtcccacgac gcgtgccact tgctgccgcg ttgcgcgatg aactgcgacc acggcagcgg
     5281 tggcgtttgt tcgttgtagc gaagttgtgg ctgcagccgc agcggcttcg tgatgttggc
     5341 ggccggcaac gcggtcaacg agtcggcgac gacctgcggg ccggacttcg gaacgtacgc
     5401 gtgcgcagtc gacgtcagcg caacaagaca gccgatccac gcggcgcggc gaacccgtac
     5461 agccatgact tgggattaga gcgcatctcg gcggattggc gaagcggtcc gattgactgc
     5521 cgctggcaaa ttgtcggagc ccgagacctc agccgaggcc tcaaaccggc ccgtagctcg
     5581 gcgacagctc acagctcggg tgctcggcca gcgagacggt gaccaagtcc gcgagcttcc
     5641 acgattgcgg cgtgccgaag gtcccgaagc tcgcaacgtg atcgggcacc caaccgacgt
     5701 cggcgacgaa ctgggcgaca tgacgcccgg ggtcagggtt cgcaaacggc acggacgccg
     5761 cgaggctgac cgagaagaat ccggtcggca tgtgatcgag ctcgtggcca tagatccgcg
     5821 cgtattcgcg gatctcgcgg cacgggtggc ggtgctcgac ctgcgagccg agaatcaccg
     5881 catcgtaatc ctgcggtggc ggcaatctgc cggcacgcgc attcgcgacg tcgacctcgt
     5941 gcccgcgcgc tcgaagccgc ccggcgaggt cttccgcgat cgcgcgagcg cgaccgatgt
     6001 aacaggcgta cgcgacgagg acccggctca tggtgcaccc acgcggtgca ttcggcgtac
     6061 cgccggatcg accatgtcga accgacaatt cgcgcagcgg ttgcgggtca acccgcgagc
     6121 acggcagcga gtgcgtcttc gcgagccgcg cgtacggcga gtccgagtgc gagctggccg
//
LOCUS       DASUFX010000086         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_13776,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000086 DASUFX010000000
VERSION     DASUFX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13776"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFV99_03370"
     CDS             complement(<1..767)
                     /locus_tag="VFV99_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058369.1"
                     /translation="MDDVLRKGGDVATLVSSWKSVDERRRKLQGELDTLRQHKNAANE
                     KMASFKDKKSAEFAAARDEMKIVSNRIKEGEATLTQLETEADDLRLAIPNAPHESVPN
                     GTSDADNTVLHTWGHKPTFSFKPQTHADLGPKLGILDFDAGAKIAGARFTVLRGAASK
                     LTRGLINYMLDLHTSHGYDEVWPPAIVRRASLRGTGQLPKFEADLFKLDIPTGPDHVA
                     DNDLFLSPTAEVQVTNLYADSIFEPGELPKRLTAYSA"
     gene            906..1460
                     /locus_tag="VFV99_03375"
     CDS             906..1460
                     /locus_tag="VFV99_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058370.1"
                     /translation="MTDSVDPDRDLAERFRSGDRSAFDLLVRKHQKGVWRIVRRYIKR
                     DADAKDVTQQVFVRAFKGLTTFRGAATVRSWLYRIAINCALSWIRDHRRELPTEIAED
                     SLTAMPIAPGRIANDQEGERLRAAIEQLPPKQKLVLELRVFDDLSFKEVAELADCSEN
                     TAKVNFHYAVKKLRDILGGADARG"
     gene            1447..1980
                     /locus_tag="VFV99_03380"
     CDS             1447..1980
                     /locus_tag="VFV99_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058371.1"
                     /translation="MREDESPELDADDRALVAKLKDLPSEGTEPNWQELEAAIRAEVG
                     DQAPRPWWRNWRWIVPVWALATTAVIALVVMCSNHEPERQAVVPAVFDAGVPSAPEQA
                     EPTTTTALWLDGEAVQLDDVPGSALDELDGEARAALTPDDDVSNGILPATDYGWLDTL
                     DDEAMVAAEDWLNRKRT"
     gene            1980..2618
                     /locus_tag="VFV99_03385"
     CDS             1980..2618
                     /locus_tag="VFV99_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058372.1"
                     /translation="MRLFLVFMLVFSSVTALAQPAPMQDKAAERREKIKQRIFALRAY
                     TLTSELQLDEATAGRLFPLLSKFDGEFEKLLVARVDITRRLRTVDTIKDPKAVNKLID
                     EAAANQRALWDIEEKRLVELRKILTPQQTARLLVVLPALERKIQNQLRKAVQRPKLRR
                     QQAPVDPFATDNDDDDDPFDDAPPAKQRPATKAPRAGKAACNPFDSLQGCAK"
BASE COUNT          496 a          889 c          924 g          474 t
ORIGIN      
        1 cacgccgagt acgcggtgag tcgcttcggc agctcgcccg gctcgaagat ggagtcggcg
       61 tagaggttcg tgacctgcac ctcggccgtt ggcgacaaga acagatcgtt gtcggcgacg
      121 tgatcgggcc cggtcggaat atcgagcttg aacagatcgg cctcgaactt cggcagctgc
      181 ccagtgccgc gcagcgacgc gcgccgcacg atcgccggcg gccacacttc gtcgtagccg
      241 tgactcgtgt gcagatcgag catgtagttg atgagcccgc gcgtgagctt cgatgcagcg
      301 ccgcgcagca cggtgaaccg cgcgcccgcg atcttcgcgc ccgcgtcgaa gtcgagaatg
      361 ccgagcttcg gtccgaggtc cgcatgcgtc tgcggcttga acgagaacgt cggcttgtgt
      421 ccccacgtgt ggagcacggt gttgtccgcg tcgctcgtgc cgttcggcac gctctcatgc
      481 ggcgcgttcg ggatcgccag gcgcagatca tcggcctcgg tctcgagctg cgtgagcgtc
      541 gcttcgcctt ccttgatgcg attgctgacg atcttcatct cgtcgcgcgc ggccgcgaac
      601 tccgccgact tcttgtcttt gaagctggcc atcttctcgt tggccgcgtt cttgtgctgg
      661 cgaagcgtgt cgagctcgcc ttgcagcttg cggcgacgct cgtccaccga cttccagctg
      721 ctcacgagcg tggcaacatc gccccctttg cgcagcacgt cgtccattcg gcttggatcg
      781 agcattcgtt acctcaataa tgaccgtggg ttggaagcgg tgtttacctt gggcttaacg
      841 caagtggcaa ggccggggtc aaatgtctcg tgcaagccct ggccctgccc gcgcttctgg
      901 agcgcgtgac agactccgta gatcccgatc gcgacctcgc cgagcgcttc cgcagcggtg
      961 atcgcagcgc gttcgatcta ctcgtgcgca agcaccagaa gggcgtgtgg cggatcgtcc
     1021 ggcgctacat caagcgcgac gctgacgcga aggacgtgac gcaacaagtg ttcgtgcgag
     1081 cgttcaaagg cctcacgacg tttcgcggcg cggccaccgt gcgcagctgg ctgtatcgga
     1141 tcgcgatcaa ttgcgcgctc agctggattc gcgatcaccg gcgcgagctg ccgacggaga
     1201 tcgccgagga ttcgctgacc gcgatgccga tcgcccccgg tcgcatcgcc aacgatcagg
     1261 agggcgagcg cctgcgcgcc gcgatcgaac aattgccgcc gaagcaaaag ctcgtgctcg
     1321 agctgcgcgt attcgacgat ctcagcttca aggaagtcgc cgagctcgcc gactgcagcg
     1381 agaacaccgc gaaggtgaac ttccactacg cggtgaagaa gctgcgtgac atcctcggag
     1441 gtgccgatgc gcgaggatga atcccccgag ctcgacgcgg acgaccgcgc gctcgtcgcg
     1501 aagctgaagg atctgccgtc cgagggtacc gagcccaact ggcaggagct cgaggccgcg
     1561 attcgcgccg aggtcggcga tcaggcgccg agaccgtggt ggcgcaactg gcgctggatc
     1621 gtgccggtgt gggcactcgc gacgacggcg gtgatcgcac tcgtcgtgat gtgcagcaac
     1681 cacgagcccg agcgacaagc ggtcgtgccg gcggtgttcg atgcgggcgt gccgtctgcg
     1741 ccggagcaag cggaacctac gacgacaacc gcgctgtggc tcgacggtga agcggtgcag
     1801 ctcgatgacg tccccggctc ggcactcgac gagctcgatg gcgaagcgcg tgcagcgctc
     1861 acccccgacg acgatgtatc gaacggaatc ttgccggcga ccgattacgg ttggctcgac
     1921 acgctcgacg atgaagcgat ggttgcggcc gaagactggc tgaacaggaa gcgaacgtga
     1981 tgcggctgtt cttggtattc atgctcgtat tctcgtcggt gacggcgctc gcgcagcccg
     2041 cgccaatgca agacaaagcc gccgagcggc gcgagaagat caagcaacgg atctttgcgt
     2101 tgcgcgccta cacgctgacg tcggagctcc agctcgacga agcgacggcc ggcaggctgt
     2161 tcccgctgct gtcgaagttc gacggcgagt tcgagaagct cctggtcgcg cgcgtcgaca
     2221 tcacgcggcg gctgcggacg gtcgacacga tcaaggaccc caaggcggtc aacaagctga
     2281 tcgacgaggc ggcggcgaac cagcgcgcgt tgtgggacat cgaggagaaa cggttggtgg
     2341 agctgcgaaa gatcctgaca ccgcagcaga cggcccgcct gctcgtcgtt ctcccggcgc
     2401 tcgagcgcaa gatccagaat cagctccgca aggcggtcca gaggcccaaa ctgcgccggc
     2461 aacaagcccc ggtcgatccg ttcgcgacgg acaacgacga cgacgacgac ccgttcgacg
     2521 acgcgccgcc tgcgaagcag cgcccggcca ccaaggcccc gcgggctggt aaggcagcct
     2581 gcaatccgtt cgactccctg cagggctgcg ccaagtagga cccggcagac agtccgcgct
     2641 atcatcgtcg ttcgatgatc gaggcgactt gtgcggcgtg cggcacggtg aaccgcatcg
     2701 ctgaagccga cgtcccgaca ggggcaaagt ttgtttcttg cacgagttgc aagtccaagg
     2761 tggcgctgcc cgcgaaggcg ccc
//
LOCUS       DASUFX010000087         6287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1680771, whole genome shotgun sequence.
ACCESSION   DASUFX010000087 DASUFX010000000
VERSION     DASUFX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680771"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFV99_03390"
     CDS             complement(<1..268)
                     /locus_tag="VFV99_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058373.1"
                     /translation="MSTDGDDPKDKKPQPPSTLAGVVPQQPPGRPGLPPPRSTHGGPP
                     RPGSSTSRPPFNPGATPRPTTPTGSPRPGTPSQSPPFSQSGATPR"
     gene            complement(318..692)
                     /locus_tag="VFV99_03395"
     CDS             complement(318..692)
                     /locus_tag="VFV99_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058374.1"
                     /translation="MQHQRTGEFRRPSDMYPLAGDDCDDLIESLDDELVEFADGTEPT
                     PRFDPADIIRQFDDIPTVNMEPPNISIARLQLVPRRVPTPFPRRERAASPTVEIEQPA
                     RPRRPSTVEIDAGYESAVRKLR"
     gene            complement(880..2025)
                     /locus_tag="VFV99_03400"
     CDS             complement(880..2025)
                     /locus_tag="VFV99_03400"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5058375.1"
                     /translation="MLLRVAIVLALVACKPKSEPAKAPPLAHAGDAGTADAAKSRTLD
                     EELEAIRSETKLPALAAAVWRNGKLVEIGAVGLRKVDDPTSKVTTKDYWHLGSNTKAM
                     TATLIGIYVDRGALHWDDTLGKLFGKVDPGYAKVTLDQILRHEGGAPGEPPDALWKQL
                     WADGDKPDARAKFVAGILAKPPAHTPGIFVYSNAGYMIAGAALEKVTKKPWQQLMRDE
                     LFAKLGMTSCGFGAPGRKDVVDEPWGHDAGGTPIAPGPAADNPPGLGPAGTVHCSLED
                     YGKFLAMHSTGEPALVTPETMQHLHAARDASTGRGYAGGWLEITTTRGTVLAHSGSNT
                     MWYVTAMVALASKPDDKWIFVMATNSGTGNAIENNFERLVRRHIVPK"
     gene            complement(2035..2700)
                     /locus_tag="VFV99_03405"
     CDS             complement(2035..2700)
                     /locus_tag="VFV99_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058376.1"
                     /translation="MRSLAILLLITSTASASPYDIGWATLGLGLGPAPDLSGDLADRL
                     ETEPCESTCPVGVRMAIGGGLGRWGVDLQIQAAAVEDTMATDYRDHDRNLFRAGPIMR
                     YTLVRSYGVDLSVRGGLQIGSLIGESSTETMPDPKCPISREGMCAPIKTTYDPEGYTI
                     FSMPLGATLRLGTRVDGGGFMGLFADFDYSIAHVSFPDDGRWGTLRSMTFGFTFGSMF
                     DLR"
     gene            complement(2801..3121)
                     /locus_tag="VFV99_03410"
     CDS             complement(2801..3121)
                     /locus_tag="VFV99_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058377.1"
                     /translation="MSEFPQPDLMDQRGRMRRIVIALLLGGAAGALGYFIADSLAKPD
                     EMIAAGKTAGSVARASQFVFYIAGFAFVVVFLVALKVQNTVADRKYRAGLVPRAEVRK
                     PPEG"
     gene            complement(3118..3678)
                     /locus_tag="VFV99_03415"
     CDS             complement(3118..3678)
                     /locus_tag="VFV99_03415"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX5058378.1"
                     /translation="MSHGERHVALLRGINVGGKNIIPMASLVKTFERLKFSSVKTFIA
                     SGNVIFEAAKQDLRKLEERIEMELEKDFDYAAKVVVKTKPEMDLIAKGIVKTWPKPSA
                     AERYYVMFLRHAINNKKLLDEFEPRPSVETLTYVPGALLWAAKIAALGKSTVAKKMLA
                     KPIYQEITVRNLNTTQKLAELMGKAK"
     gene            complement(3695..4108)
                     /gene="atpC"
                     /locus_tag="VFV99_03420"
     CDS             complement(3695..4108)
                     /gene="atpC"
                     /locus_tag="VFV99_03420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX5058379.1"
                     /translation="MAASFLAVNLVTPKGVVAHTDTDSVQAPGELGEFELLPGHVPML
                     TALKPGVLTVGSKQRARYAVSTGYLRIDPQGAVEILVEQAQPATEVDPEAARSDLKTA
                     ETELAKWGDKAQDGDYQNLVARAGWAKARLDAASH"
     gene            complement(4122..5537)
                     /gene="atpD"
                     /locus_tag="VFV99_03425"
     CDS             complement(4122..5537)
                     /gene="atpD"
                     /locus_tag="VFV99_03425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5058380.1"
                     /translation="MVQVIGPVVDVAFDSAELPEINTALLLTNKAIDSREDNLTVEVA
                     QHLGEKMVRCVAMDTTDGLVRGQSVKNTNAAISMPVGPEVLGRILNVVGNPVDEAGPV
                     NAKQRSPIHRSAPKFTDQSVSVEMFETGIKVIDLLAPYRKGGKIGLFGGAGVGKTVLI
                     MELINNTAKKSGAYSVFAGVGERTREGNDLYHELADSKLFDGSTVLSKTALIYGQMNE
                     PPGARQRVAISALTVAEYFRDILKQDLLLFVDNIFRFTQAGSEVSALLGRIPSAVGYQ
                     PTLSTEMGQLQERITSTKDGSITSIQAIYVPADDLTDPAPATAFAHLDATTVLNRAIT
                     EKGIYPAVDPLDSTSSILSPAVVGDRHYKVAREVQRILQRYKDLQDIIAILGMDELSE
                     DDKLTVSRARKIEKFFGQPFDVATTFTGMKGIYVSRDDTVRSFEEVLAGKCDDLPEQA
                     FEMTGTIDDVRAKAAELAKRA"
     gene            complement(5580..>6287)
                     /gene="atpG"
                     /locus_tag="VFV99_03430"
     CDS             complement(5580..>6287)
                     /gene="atpG"
                     /locus_tag="VFV99_03430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX5058381.1"
                     /translation="SGVAGKDAHPLFEEREAKRAAIVVITADRGLAGAFNTNVIKAVE
                     RYSHNELAGASEITLRIIGKKANQHFARRNANIASYDPAPTGPTALTTAREAANRVIA
                     DFLDNKVDRVFLAYNEFKNAGTNIVRVVQILPVSPGSTDSGDETTQATDYIYEPSKEA
                     LLSRLVPLYVQIQIYRAALESIAAFFGAQMMAMESATKNAGEMINRYTLQYNRARQAA
                     ITKELLEIIGGAEALKG"
BASE COUNT          998 a         2067 c         2058 g         1164 t
ORIGIN      
        1 gccgcggtgt cgcgcccgat tgcgagaacg gtgggctctg cgacggcgta cccggccgcg
       61 gcgaacccgt cggtgttgtc ggccgcggcg tcgcgccggg attgaacggc ggccgcgacg
      121 tcgacgatcc aggacgcggc ggtccaccgt gcgtcgagcg cggcggcggc aggcctggtc
      181 gtcccggcgg ctgttgcggc acgacaccgg caagtgtcga cggcggctgc ggcttcttgt
      241 ctttcggatc gtccccgtcc gtggacatgg gcgcaatggt accggcgcta cctgtgcgtc
      301 gctagcgcgg aggcccgtta ccgcagcttg cggacggcag actcgtagcc cgcatcgatc
      361 tcgacggtcg acggcctgcg cggccgagcc ggctgctcga tctcgacggt tggacttgcg
      421 gcgcgctcgc ggcgcggaaa tggcgtcggc acgcggcgcg gcacgagctg caggcgcgcg
      481 atcgagatgt tcggcggctc catgttgacc gtcgggatgt cgtcgaactg ccggatgatg
      541 tctgccggat cgaagcgggg cgtgggttcc gttccgtcgg cgaattcgac gagctcgtcg
      601 tcgagcgatt cgatgaggtc gtcgcagtcg tcgccggcga gcggatacat gtcgctcggc
      661 cgcctgaact cgccggttcg ttggtgttgc aagggatcgt gaacgtaggg atccgtctga
      721 tcccccactt ctagctgctc tagcgctgtt actaccgctg gcatcgaagc tgatttgtgc
      781 aatgcgcgga ccgcgcataa ctgccgacaa ttcggtgcga atacccacag ccccatgtcc
      841 gtcgcctgca caactgtgca agcagcgtcg ggtcgcgggc tatttcggca cgatgtgtcg
      901 acgcacgagt cgctcgaagt tgttctcgat cgcgttgccg gtaccgctgt ttgtcgccat
      961 cacgaagatc catttgtcgt cgggcttcga cgcaagcgcg accatcgcgg tcacgtacca
     1021 catcgtgttg ctgccgctgt gcgcgagcac cgttccacgc gtcgtcgtga tctcgagcca
     1081 accgcccgcg tagccgcgcc cggtcgacgc gtcgcgcgcg gcatgcaagt gttgcatggt
     1141 ctccggggtg accagcgcgg gctcgccggt cgagtgcatc gcgaggaact tgccgtagtc
     1201 ctcgagcgag cagtgcacgg tgcccgcggg cccgagtcca ggaggattgt ccgcggccgg
     1261 tcccggcgcg atcggcgtgc cgccggcgtc gtgtccccac ggctcgtcga caacatcctt
     1321 ccgccccggc gcgccgaacc cgcacgacgt catgccgagc ttcgcgaaca gctcgtcgcg
     1381 catgagctgt tgccacggct tcttcgtcac cttctcgagc gcggcgccag cgatcatgta
     1441 ccccgcgttc gagtacacga atatgccggg cgtatgcgcg ggcggcttcg cgagaatgcc
     1501 agcgacgaac ttcgcgcgcg catcgggctt gtcgccgtcg gcccacagct gcttccacag
     1561 ggcatccggt ggctcgcctg gcgcgccgcc ctcgtggcgt aggatctgat cgagcgtgac
     1621 cttcgcgtaa ccgggatcga ccttgccgaa cagcttgccg agcgtatcgt cccagtgcag
     1681 tgcgccgcga tcgacataga tgccgatcag cgtcgcggtc atcgccttcg tgttcgaacc
     1741 gaggtgccag tagtccttcg tcgtcacctt gctcgtcgga tcgtcgacct tgcgcagccc
     1801 gaccgcaccg atctcgacga gcttgccgtt gcgccacacc gcagcagcga gcgccggcag
     1861 tttcgtctcg ctgcgaatcg cctcgagctc ctcgtcgagc gtgcgcgact tggcggcgtc
     1921 tgcagttccg gcatcgcccg catgcgcgag aggtggcgcc ttcgccggct cgctcttcgg
     1981 tttgcaggca acgagcgcca ggacgatcgc gacacgcagc agcatgccgc gacactaccg
     2041 caggtcgaac atcgaaccga acgtgaagcc aaacgtcatc gaccgcagcg ttccccaccg
     2101 gccgtcgtcc ggaaagctga cgtgcgcgat cgaataatcg aagtcggcaa acagccccat
     2161 gaagccgccc ccatcgacac gtgtgccgag ccgcagcgtc gcgccgagcg gcatcgagaa
     2221 gatcgtgtag ccctctgggt cgtacgtcgt cttgatcggc gcgcacatac cctcgcggct
     2281 aatggggcac ttcggatccg gcattgtctc cgtcgaggac tcgccgatca gcgaaccgat
     2341 ctggagacca ccgcgcacgg agaggtcgac gccgtaactg cgaacgagcg tgtagcgcat
     2401 gatcggtccg gcgcgaaaca ggttgcgatc gtgatcgcgg tagtccgtcg ccatcgtgtc
     2461 ctcgacggca gctgcttgga tctgcaagtc gacaccccag cgaccaagcc cgccgccgat
     2521 cgccatcctc acgccaaccg ggcacgtcga ctcgcacggc tcggtctcga gacgatcggc
     2581 gaggtcgccg gagagatccg gcgctgggcc gagcccgagc ccaagcgtcg cccagccgat
     2641 gtcgtacgga gaggccgagg ccgtggatgt gatcaacagc aagatcgcga gtgagcgcac
     2701 ggcgacgcat tcagcaacac acgcaccacg cgcacggctg ggttcgcatc cacttagccg
     2761 gcgctcgtcg cacggcaaac gccagctcgc gacatcgctg tcagccctca ggcggcttgc
     2821 gaacttcggc gcgcggtacg agcccggcgc ggtacttgcg gtcggcgacc gtgttctgga
     2881 ccttgagcgc aaccaggaac acgacgacga atgcgaaccc tgcgatgtag aacacgaact
     2941 gcgaggcgcg tgcgacgctg cccgcggtct tgccggcggc gatcatctca tcgggtttcg
     3001 cgagcgaatc ggcgatgaag tagccgagcg cgccggccgc accaccgagc aagagcgcga
     3061 tcacgatgcg ccgcatgcga ccgcgctggt ccatcagatc tggctgcgga aattcgctca
     3121 cttggccttc cccatcagct cggcgagctt ctgcgtcgtg ttgaggttgc gcacggtgat
     3181 ctcctggtaa atcggcttcg cgagcatctt cttcgcgacc gtgctcttac cgagcgcagc
     3241 tatcttcgcc gcccacagga gcgcgcccgg cacgtacgtc agcgtctcga cactggggcg
     3301 cggctcgaat tcatcgagca gcttcttgtt gttgatcgcg tggcgcagaa acatcacgta
     3361 gtagcgctca gccgcactcg gcttcggcca cgtcttcacg atgcctttcg caatcaggtc
     3421 catctcgggc ttcgtcttca cgacgacctt cgccgcgtaa tcgaagtcct tctcgagctc
     3481 catctcgatc cgctcttcga gcttgcggag atcctgcttc gctgcttcga agatgacgtt
     3541 gccgctcgcg atgaacgtct tcacggatga aaacttcagc cgctcgaacg tcttcacgag
     3601 cgacgccatc gggatgatgt tcttcccgcc gacgttgatg ccgcgcagta gcgcgacgtg
     3661 ccgctcgccg tggctcatgc ggcgagcgta gcgcttagtg cgacgcggcg tcgaggcggg
     3721 ccttcgccca gcccgcgcgt gccacgaggt tctgatagtc gccgtcttga gccttgtcgc
     3781 cccacttggc gagctcggtc tcggcggtct tcagatcgct gcgcgcagcc tcgggatcga
     3841 cctcggtcgc aggctgtgcc tgctcgacga ggatctcgac cgcgccttgc ggatcgatgc
     3901 gcaggtagcc ggtcgacacc gcgtagcgcg cacgctgctt gctgccgacg gtgagcacgc
     3961 ccggcttgag cgcggtcagc atcggcacgt gtcccggcag cagctcgaac tcgccgagct
     4021 cgcccggcgc ctgcacgctg tcggtatccg tgtgcgcgac gacgcccttc ggcgtcacca
     4081 ggttgactgc gagaaacgac gcggccattg gacgatctcc ctcaggccct cttcgccagc
     4141 tcggccgcct tcgcgcggac gtcgtcgatc gtgccggtca tctcgaacgc ctgctcgggg
     4201 aggtcgtcgc acttgccggc gaggacttcc tcgaagctgc gcactgtgtc gtcgcgcgac
     4261 acgtagatgc ccttcatgcc ggtgaacgtc gtcgcgacgt cgaacggctg accgaagaac
     4321 ttctcgatct tgcgcgcgcg cgacacggtg agcttgtcgt cttccgagag ctcgtccatg
     4381 ccgagaatcg cgatgatgtc ctgcagatcc ttgtagcgct gcaggatgcg ctgcacttcg
     4441 cgggcgacct tgtagtgacg gtcgccgaca acggcgggcg agagaatcga cgacgtcgag
     4501 tcgagcggat cgaccgccgg gtagatgccc ttctccgtga tcgcgcggtt gagcaccgtc
     4561 gtcgcgtcga ggtgggcgaa cgccgtcgcc ggcgccgggt cggtcaagtc gtcggcgggc
     4621 acgtagatcg cttggatcga cgtgatcgag ccgtccttcg tcgacgtgat gcgctcctgg
     4681 agctgaccca tctcggtcga cagcgtcggc tgataaccga cggcgctcgg gatacggccg
     4741 agaagcgccg acacctcgga gcccgcctgc gtgaagcgga agatgttgtc gacgaacagg
     4801 aggaggtcct gcttgaggat gtcacggaag tactcggcga ccgtgagcgc cgaaatcgcg
     4861 acgcgctggc gcgcaccggg cggctcgttc atctggccgt agatcagcgc cgtcttcgag
     4921 agcaccgtcg agccgtcgaa tagcttcgag tcggcgagct cgtgatagag gtcgttgcct
     4981 tcacgcgtgc gctcgccgac accggcgaac accgagtacg cacccgactt cttcgcggtg
     5041 ttgttgatga gctccatgat gagcacggtc ttgccgacgc ctgcgccgcc gaacagaccg
     5101 atcttgccgc ccttacggta tggcgcgagg aggtcgatga ccttgatgcc ggtctcgaac
     5161 atctcgacgc tgacgctctg gtcggtgaac ttcggtgccg agcggtggat cggcgaccgc
     5221 tgcttcgcgt tgaccgggcc cgcttcgtcg acggggttac cgacgacgtt caggatgcgg
     5281 ccgagcactt cggggccgac cggcatcgag atcgcggcgt tcgtgttctt caccgactga
     5341 ccgcggacga gaccgtccgt cgtgtccatc gcgacgcagc gaaccatctt ctcgccgagg
     5401 tgctgagcga cctcgacggt gaggttgtct tcgcggctgt cgatcgcctt gttggtgagc
     5461 aacagcgcgg tgttgatctc cggcagctct gccgaatcga aggccacgtc gacgacggga
     5521 ccgatgacct ggaccacgcg gcccaacgag ttaggagaaa aatcggtagc catgatgcgt
     5581 taccccttga gagcctcggc gccgccgatg atctcgagca gctccttggt gatcgccgcc
     5641 tgacgagcgc ggttgtactg aagcgtgtag cgattgatca tctcgccggc gttcttcgtc
     5701 gcgctctcca tcgccatcat ctgggctccg aagaacgcag cgatcgattc gagcgccgcg
     5761 cgatagatct ggatctggac atagagcgga acgaggcgcg acagcagcgc ctccttcgag
     5821 ggctcgtaga tgtagtcggt cgcctgcgtc gtctcgtcgc cggaatctgt agacccgggc
     5881 gaaaccggca ggatctggac gacgcgcacg atgttggtgc ccgcgttctt gaactcgttg
     5941 tacgcgagga agacgcggtc gaccttgtta tcgaggaagt cggcgatgac acggttcgcc
     6001 gcttcgcgag cggtcgtgag cgccgtcggc ccggtgggcg ccgggtcgta gctcgcgatg
     6061 ttcgcgttgc gacgagcgaa gtgctggttc gccttcttgc cgatgatgcg gagcgtgatc
     6121 tccgacgcgc cggccagctc gttgtgcgag tagcgctcga cggccttgat cacgttcgtg
     6181 ttgaacgcgc cggcgagacc gcggtccgcc gtgatcacga cgatcgcagc ccgcttcgcc
     6241 tcacgctctt cgaacagcgg gtgcgcgtcc ttaccggcga cacccga
//
LOCUS       DASUFX010000088         5770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_430626,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000088 DASUFX010000000
VERSION     DASUFX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430626"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VFV99_03435"
     CDS             <1..821
                     /locus_tag="VFV99_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5058382.1"
                     /translation="PQVRMEACAALGELREPSAVPHLIKRLDDGAAPVRQNAAIALGT
                     IGHPDGFSPLADALNGGPADLRFQAATSLAEIDPARAFALVIAALGDKDAEVVGAAAL
                     SVGAIAKLDETKRAPAIAALTGKIDHATPGTRFDVAYALAELGDPAGRSALSAALGDM
                     DRAWDAVSALGELQAKEELAKAVATRTAPMEARVLAAGKLLALGVDSDEARALLLEAL
                     GVRKVNVRGLAVEQLGEIGGTWAKQPLEKLARSGKGTELLEPIALALRAIEARK"
     gene            818..1603
                     /locus_tag="VFV99_03440"
     CDS             818..1603
                     /locus_tag="VFV99_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5058383.1"
                     /translation="MIELFDSHAHVDGPEFDADREAVMQRARDAGVKRIVVIGAVGDP
                     SSAERSVALAEKDSNVWATIATHPHDVKQMTDDWWATHERLARHPRVVAIGETGLDYY
                     YDHSPHDTQQAAFRRFIELAHAVKKPVVCHIRDAHDDARAILREGRAAELGCVIHCFT
                     GTPDDAREYAAMGCYVSFSGIVTYKTAQNLRDSVPLVPRERLMIETDCPYLAPVPKRG
                     KRNEPAFVVHTAEVVAQCAGLSFEDLARITTENASRVYRLPTG"
     gene            1663..2400
                     /gene="rplC"
                     /locus_tag="VFV99_03445"
     CDS             1663..2400
                     /gene="rplC"
                     /locus_tag="VFV99_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5058384.1"
                     /translation="MALNIGLLGKKIGMTQVFADDGEAVPVTVIQAGPCHVIGTRTQE
                     RDGYSALVLGFDEKPLRNANKAELGAVKANGGKPQRFIREIRLKADEVAKFQVGQVLG
                     PKDVFAENIAVDVEGWSKGKGYQGVIKQHHMSGMTRAHGTHEYFRHGGSIGCRLTPQR
                     VHKGKRMAGQMGNEKVTQQNLQLFKILADENCILVRGAVPGANNEYVVVTKAATRTTY
                     KRKGVGKEEVRSKNPLKASKKAAAGRG"
     gene            2458..3057
                     /locus_tag="VFV99_03450"
     CDS             2458..3057
                     /locus_tag="VFV99_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX5058385.1"
                     /translation="MSKLDDRSTRRDRFHQKAKKQGFLARAVFKLEELDKQFKLFKPG
                     QRVLDLGCAPGSWLQYARTKVGDKGVLVGLDRGPLKGDVAGARIVVGDVMSIDVAELK
                     GELPAFDVVLSDMAPDTSGIRHLDQARSEALFERALEIATLTLAPGGNFVGKLFQGPD
                     FKKLIEAVRAKFETAKSAKPDSSRQISIEQYVIGKGYKP"
     gene            3054..3728
                     /locus_tag="VFV99_03455"
     CDS             3054..3728
                     /locus_tag="VFV99_03455"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5058386.1"
                     /translation="MTLRVVIEQWGEPNPRKIEQAVEVLHRGGVAAYPTDSIYALGCA
                     IEARDAIEKIFRAKQMNKNQRLALICPDLSTASEFGLFSQTAFRLAQRIFPGPYTLVV
                     PATRVVPRTLTDHKRRTVGIRITSHPIAQAIAKELGRPLLTTSAISPETHEPCRDAEE
                     ILEAFGKFVDVVVDAEQTPAEPSTVLEVDGEEIKLLRLGQGSIEGLEVIEETPEDNRK
                     VAGRRR"
     gene            complement(3766..4662)
                     /locus_tag="VFV99_03460"
     CDS             complement(3766..4662)
                     /locus_tag="VFV99_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058387.1"
                     /translation="MRCTGSVLVVACLLRAVSSSAQPLPPQPPQDPAAPPPSQPLPPT
                     APQSSQSPSDPAAPPPSQPLPPTAPPPSQQLPPTTPQSSQPPPDPAAPPPLPPTAPPQ
                     LPPPVTPANVDHEQPKPREYRSILGIGVSGTLKPDWDQRFTIATELERNVHTGRFHLG
                     WNIHWSFRNWSVIDAVYKQISADDISAPGKAFGYVFGWISGTSMLGAGWQLRYAQEDG
                     AKGWALLGTRVVLFLDPIAGGEGDDVDASLSHALHGAIGYDFGDFGLGLRAMWAPDAL
                     LFGSTDSAPLFLSMATLELRKE"
     gene            complement(4803..5231)
                     /locus_tag="VFV99_03465"
     CDS             complement(4803..5231)
                     /locus_tag="VFV99_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058388.1"
                     /translation="MRRRARSRAGAIAVAVALAACSRDTAKAPAHEPAATVKPPAKQL
                     VEPPRPEQLAHVTIKALGMYCEESCPLKVRYALADVKAVYELGFDVSNESIFVSYDAT
                     LGSAKDVTKPMIAAIKSAGFNPWLSKEEWPADAKAQVVLR"
     gene            complement(5228..>5770)
                     /locus_tag="VFV99_03470"
     CDS             complement(5228..>5770)
                     /locus_tag="VFV99_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058389.1"
                     /translation="DMKNAQPALEVAVKDLGEDAPNPLQYRTRTALAEIYLAAGNIPE
                     AGKQLDEALKINPGYFPTRVLQAKVVLRNDDPDRALNLLVPIRNEYTSTEIDLLTIEA
                     GCASKKTTAKEKDEAKGALAKMKDAPNAPLAEMSRVAAICDPDLPKQLGLPEAGKAGS
                     SSKPDKPAKSTKSSGKRRRK"
BASE COUNT         1001 a         1874 c         1949 g          946 t
ORIGIN      
        1 atccgcaggt ccgcatggaa gcgtgcgccg cgctcggcga gctccgcgag cccagtgcag
       61 tcccgcacct gatcaagcga ctcgacgacg gtgctgcgcc ggtgcgccag aacgcggcga
      121 tcgcgctcgg cacgatcggt catcccgacg ggttttcgcc tctggccgac gcgctgaacg
      181 gcggaccggc cgatttgcgc tttcaagcgg cgacgtcgct cgccgagatc gatccggcgc
      241 gtgcgttcgc tttggtgatc gcagcgctcg gtgacaagga tgccgaggtt gtcggtgcgg
      301 ccgcgttgtc ggtcggcgcc atcgcaaagc tcgacgaaac caagcgcgcg cccgcgatcg
      361 cagcgctcac cggcaagatc gatcacgcga cgccgggcac tcggttcgac gtcgcgtacg
      421 cgctcgccga gctcggcgat ccggcgggcc gcagcgccct ttccgcagca ctcggcgaca
      481 tggatcgcgc atgggacgcg gtgagcgcgc tcggcgagct gcaggcgaag gaagagctcg
      541 cgaaagcggt cgcgacgcgc actgcgccga tggaagcacg cgtgctcgcg gcaggtaagc
      601 tgctcgcgct cggcgtcgat agcgacgagg cacgcgcgct gctgctcgaa gcactcggcg
      661 tgcgcaaagt gaacgtgcgt ggcctcgccg tcgagcaact cggcgagatc ggcggcacct
      721 gggcgaagca accgctcgag aagctcgccc gctcgggcaa gggcaccgag ctgctcgagc
      781 cgatcgcgct cgcgctccgt gcgatcgagg cgcgcaaatg atcgagctgt tcgattcaca
      841 cgcgcacgtc gacggtcccg agttcgacgc cgatcgcgaa gcggtgatgc aacgcgcgcg
      901 cgatgccggc gtgaagcgga tcgtcgtgat cggtgcggtc ggcgatccgt cgagtgcaga
      961 acgctcggtc gcgctcgccg agaaagattc gaacgtgtgg gcgacgatcg cgacgcaccc
     1021 gcacgacgtg aagcagatga cggacgactg gtgggcgacg cacgagcggc tcgcgcgcca
     1081 tccgcgcgtc gtcgcgatcg gcgagaccgg gctcgactac tattacgacc actcgccgca
     1141 cgacacgcag caggccgcgt tccgccgctt catcgagctc gcgcacgcgg tgaaaaaacc
     1201 ggtcgtctgc cacatccgcg atgcccacga cgatgcgcgc gcgatcctgc gcgagggccg
     1261 cgcggccgag ctcggctgcg tgattcactg cttcacaggc accccggacg acgcccgcga
     1321 gtacgcggcg atgggctgct acgtctcgtt ttcgggcatc gtcacgtaca agacggctca
     1381 gaatcttcgg gactcggtgc cgctcgttcc ccgggaacgg ctgatgatcg agaccgactg
     1441 cccctacctg gcgccggtcc ccaagcgcgg caagcgcaac gaaccggcct tcgtcgttca
     1501 cacggcggag gtcgttgccc agtgcgccgg cttatctttc gaggacttag cccggatcac
     1561 caccgagaac gcctcccggg tctaccggct accaactggt tgactcccaa ggacttggcc
     1621 ggtatagatt cgctcctcgt ttctggaaga gagaagtctg ccatggcact caacatcggc
     1681 ctgctcggca aaaagatcgg tatgacccag gtgttcgcgg acgacggtga agccgtcccc
     1741 gtgaccgtga tccaggccgg accgtgccac gtgatcggta cccgcaccca agagcgcgac
     1801 ggctattcgg ccctcgtgct cggcttcgac gagaagccgc tccgcaacgc caacaaggcc
     1861 gagctcggcg cggtgaaggc gaacggcggc aagccgcagc gcttcattcg cgagattcgc
     1921 ctcaaggcgg atgaagtcgc gaagttccag gtcggccagg tactcggccc gaaggacgtc
     1981 ttcgccgaga acatcgcggt cgacgtcgag ggctggagca agggcaaggg ctaccagggc
     2041 gtcatcaagc agcaccacat gtcgggcatg actcgcgcgc acggtacgca cgagtacttc
     2101 cgccacggcg gatcgatcgg ttgccgcctc accccgcagc gcgtccacaa gggcaaacgc
     2161 atggcgggcc agatgggcaa cgagaaggtc acgcagcaga acctccagct gttcaagatc
     2221 ctcgcggacg agaactgcat cctcgtgcgc ggcgcagtgc cgggcgcgaa caacgaatac
     2281 gtcgtcgtca ccaaggcggc gacgcgcacc acctacaagc gcaagggcgt tggtaaggaa
     2341 gaggtgcgtt cgaagaaccc gctgaaggcg tcgaagaagg cggcagcggg tcgcggctaa
     2401 ccagccaacc aggcagttgt ttacgcccgg cacgttcgtg tcgggcgttt gtgttttatg
     2461 tccaagctcg acgacagaag tactcgccgc gatcgctttc accagaaggc gaagaagcag
     2521 ggcttcctcg cgcgcgcggt gttcaagctc gaagagctcg acaagcagtt caagctgttc
     2581 aagccgggtc agcgcgtgct cgatctgggc tgcgccccgg gctcgtggct gcagtacgcg
     2641 cgcacgaagg tcggcgacaa gggcgtgctg gtcggtctcg atcgcgggcc actgaagggt
     2701 gacgtggctg gcgcgcggat cgtcgtcggt gacgtgatgt cgatcgatgt cgccgagctc
     2761 aagggcgagc tgccggcgtt cgacgtcgtg ctgtcggaca tggcgcccga tacgtcggga
     2821 attcgccatc tcgatcaggc gcgcagcgag gcgctgttcg aacgcgcgct cgagatcgcg
     2881 acgctcacgc tcgcgccggg cggcaacttc gtcggcaagc tgttccaggg cccggacttc
     2941 aagaagctca tcgaagcggt gcgcgcgaag ttcgagaccg cgaagtccgc gaagcccgac
     3001 tcgagccgtc aaatctcgat cgagcagtac gtgatcggga aaggctacaa accgtgaccc
     3061 tgcgcgtggt catcgagcag tggggcgagc cgaacccgcg caagatcgag caggccgtcg
     3121 aggtgctcca tcgtggcggc gtggccgcct accccaccga ttcgatttac gcgctcggct
     3181 gcgcgatcga agcgcgcgac gcgatcgaga agatcttccg cgcgaagcag atgaacaaga
     3241 accagcggct cgcgctgatc tgccccgacc tgtcgaccgc gtcggagttc ggtctgttct
     3301 cgcaaaccgc atttcgcctc gcgcagcgga tcttccccgg cccgtacacg ctcgtcgtgc
     3361 cggcgacgcg agtcgtgccg cgcacgctca ccgatcacaa gcggcgcacg gtcggtatcc
     3421 gcatcacgag ccatccgatc gcccaggcga tcgcgaagga gctcggccgc ccactgctca
     3481 cgacgagcgc gatttcgccg gagacgcacg aaccgtgtcg cgatgccgaa gagatcctcg
     3541 aggcatttgg caagttcgtc gatgtcgtcg tcgatgccga gcagacgccg gcggagccct
     3601 cgacggtgct cgaagtcgat ggcgaggaga tcaagctgct gcgcctcggc caaggatcga
     3661 tcgaaggcct cgaagtgatc gaggagacgc cggaagacaa tcgcaaggtc gccgggcgtc
     3721 gccgctagcg gccagactcg tatcgcagca ggccttggcg ctgcatcact ccttacggag
     3781 ctcgagcgtc gccatgctca ggaacagcgg cgcgctatcc gtcgagccga acagcaaggc
     3841 atcgggtgcc cacatcgcgc gcagaccaag cccgaagtcg ccgaagtcat agccgatggc
     3901 cccatggagc gcgtgcgaca gcgacgcgtc gacgtcgtca ccctcgccgc ccgcaatcgg
     3961 atcgaggaag agaacaacgc gcgttccaag taatgcccag cccttcgcgc catcctcttg
     4021 cgcgtacctg agttgccagc ccgcaccgag catcgaggtt cctgagatcc aaccgaagac
     4081 gtagccgaac gccttcccag gagcgctgat gtcgtcggcg ctgatctgtt tgtagacggc
     4141 gtcgatcacg ctccagttgc gaaacgacca atgaatgttc cagccaagat gaaaacggcc
     4201 cgtatgcacg ttgcgctcga gctcggtcgc aatcgtgaac cgctgatccc agtctggctt
     4261 cagggtgccc gaaacgccga ttccaaggat gctgcgatac tcgcgtggct tcggctgttc
     4321 gtgatcgacg ttcgcaggcg tgacaggagg aggaagctgc ggaggcgcgg tcggaggtag
     4381 cggcggaggc gcggccggat ccggtggtgg ctgcgaggat tgaggcgtgg tgggcggtag
     4441 ctgctgcgac ggcggaggcg cggtgggtgg tagcggctgc gacggcggag gcgcggccgg
     4501 atccgatggt gactgcgagg attgaggcgc ggtgggtggt agcggctgcg acggcggagg
     4561 cgcggccgga tcctgtggtg gctgtggcgg tagtggctgt gcgctcgacg acaccgcgcg
     4621 cagcaggcac gcaacgacca gaacgctccc cgtgcacctc attcctccaa ggatcaccgt
     4681 ttgctgctgt ggcgacaact tgcaaagcgc ggcaacggac gagcttaccg ggttgtattt
     4741 cggcccggca ttgacctcgt ccgacagcgg acgcgccgcg accaccgacc gtgcccggac
     4801 ccttagcgca gcacgacctg ggccttcgcg tccgctggcc actcttcttt cgagagccac
     4861 ggattgaagc ccgccgattt gatcgcggcg atcatcggct ttgtcacgtc tttcgccgaa
     4921 ccgagcgtcg cgtcgtacga gacgaagatg ctctcgttcg agacgtcgaa gccgagctcg
     4981 tagaccgcct tcacgtcggc gagcgcgtag cgcaccttga gcgggcagct ctcctcgcaa
     5041 tacatgccga gcgccttgat ggtgacgtgc gcgagctgct cgggacgcgg cggctcgacg
     5101 agctgcttgg ccggcggctt caccgtcgcg gcaggctcgt gcgcgggcgc cttcgctgtg
     5161 tcgcgactgc acgctgcaag cgcgaccgcg acggcgatcg cacctgcgcg cgagcgagcg
     5221 cgacgcctca tttgcggcgg cgcttgcccg acgacttggt ggacttggcc ggcttgtccg
     5281 gcttgctgct gctgcccgcc ttgcccgctt ccggcaggcc gagttgcttc ggcaggtccg
     5341 gatcgcagat cgccgcgaca cgagacatct cggcgagcgg tgcgttcggc gcatccttca
     5401 tcttcgcgag cgcgcccttc gcttcgtcct tttccttcgc cgtcgtcttc ttgctcgcgc
     5461 agccggcttc gatggtgagc agatcgatct cggtcgacgt gtactcgttg cgaatcggca
     5521 cgagcaggtt gagcgcgcga tccgggtcgt cgttgcggag cacgaccttc gcctgcagca
     5581 cgcgcgtcgg gaagtagccc gggttgatct tcagtgcctc gtcgagctgc ttgcccgcct
     5641 cggggatgtt gccggctgcg aggtaaatct cggcgagcgc ggtgcgcgta cggtactgca
     5701 gcgggttggg cgcgtcctcg ccgaggtcct tcaccgccac ttcgagcgcg ggctgcgcgt
     5761 tcttcatgtc
//
LOCUS       DASUFX010000089         5600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_430883,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000089 DASUFX010000000
VERSION     DASUFX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430883"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFV99_03475"
     CDS             <1..331
                     /locus_tag="VFV99_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058390.1"
                     /translation="GALIGEHDFSAFRAAGCTAKTTMRRVDAIALTRAQPHLLDIDIR
                     GNAFLRQMVRIIVGTLAEVGTGKRSVAQVAEILASKDRTQAGITAPAHGLELMEVRYD
                     GVRPRRV"
     gene            365..982
                     /locus_tag="VFV99_03480"
     CDS             365..982
                     /locus_tag="VFV99_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5058391.1"
                     /translation="MASEPATQVELEEEQRQVEEAQAGNLDAMRPILERYAQPLYGTV
                     ILPRLGDAASAEEVLRDTLATAVEKIRRFTWQGKSIYPWLRQIAINKVYDVHRQSKRS
                     RRLADAMVHEVPIETDPDSHADAQLIADQERAAHRVRIDDAMAQIAERYRVAIELRLI
                     QELSREDCAKKLGITIGTFDVLLFRAVRAFRKHFGERVEGESDGD"
     gene            972..1565
                     /locus_tag="VFV99_03485"
     CDS             972..1565
                     /locus_tag="VFV99_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058392.1"
                     /translation="MATDDDDILTGPVPAPDAEATPTERAQAKTFADIVDKTLAGRTP
                     PAMSAEDRSLLEVATVIRAATGKAELSATKRRSLVEDALRQAVGGAGTAVASGVTPIS
                     RARKRWVPWAIAGASTLVAAAAILLLLLRTPHVPNTAPPVAPPPIAENWKSRPADPLI
                     GPISREHAGDAATRIDAIYADRLDGYRERTLARGRTP"
     gene            1562..2134
                     /locus_tag="VFV99_03490"
     CDS             1562..2134
                     /locus_tag="VFV99_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058393.1"
                     /translation="MKTYLAVLVAAVIAITGWISCGSDKDQTTPTTKTPEPTDVIGKD
                     LMISLSKAKNFHHKARVLMSDGKFAEAIASVREILSLQFPKNAPEAEDVRNDARALLA
                     KLLVGQGQVEEAMTVVTEGIAQSQRESFFVANLYTVKGEVHEARAALLDANDPNTKAK
                     IVEEKRAAIQAYDKSNEINTELQKTLMEER"
     gene            2131..2583
                     /locus_tag="VFV99_03495"
     CDS             2131..2583
                     /locus_tag="VFV99_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058394.1"
                     /translation="MRASTLGVVLLTAIMIAIACGPKQPPNGPKQDEITALWIQIRDW
                     RREAGLPVDPRREDVISIGPLTIDQAKHVCPDSHPVPPSCGDVCSLAENICDNAESIC
                     NLADELGKDDKWAQDKCSNAKASCRDAKKRCCQCSETDSKSASKASTW"
     gene            2577..3029
                     /locus_tag="VFV99_03500"
     CDS             2577..3029
                     /locus_tag="VFV99_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058395.1"
                     /translation="MVKAWPAALFCALVVACAAAPKPMQTEAPPTKNHDGGELLPAGK
                     KQQIEQLEAAIAADRDKLELAEPAQPDYINTRPEPMGTTPAVDNPSCKPAKTDKCTTS
                     CTLSNSICTNADSICRIAVELDDDWSRGRCAKANKTCEGARDKCCGCQ"
     gene            3011..3478
                     /locus_tag="VFV99_03505"
     CDS             3011..3478
                     /locus_tag="VFV99_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058396.1"
                     /translation="MLRLSVIALLVVGVSCGGRKMGAKAPATPSESAPAEAGAAPSIL
                     APSTHDEIEKLDAEITASRQQLKLDEPTEQAIQAAPTQALGAMPSTQDTKCHPAQNDT
                     CKTSCTLSDSICSNANKICNLANSMPGDNWAMNKCAKANTTCESSKTKCCGCQ"
     gene            3739..4164
                     /locus_tag="VFV99_03510"
     CDS             3739..4164
                     /locus_tag="VFV99_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058397.1"
                     /translation="MSSVLADETEIEIDELWRRCAKGYGSSAERDPWLHASRTDHPGH
                     HGLVYILRSTYHLDAVMIARHDPVESAIVNYCGPAQDRDLMAELGAHVIADLARRKTV
                     RIRAATTHAPMMDALESIGFVHSRRPGAAVSARIVAYGP"
     gene            4154..5152
                     /locus_tag="VFV99_03515"
     CDS             4154..5152
                     /locus_tag="VFV99_03515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5058398.1"
                     /translation="MVREATNRAPEFTVRLASAADGPTIVAAVRELLPGVDVAQRHMW
                     LYRENPHGEALTWIATEEATGAVAGVTSFFPRRIVANGREVLAALGGDGYVRPAFRRR
                     GLASAMHGMSRRDMQRFGIEVMFGTPMPANATPLARHGTQNVVEVVRYSRWLGGWLAP
                     IGAFVRRALRAHVRLDEFSERDWRVDELWERTRPELGIATIRDAQFYDWRFRRSPSQR
                     QQPFVVLDRGRPIAACALERVGRRLRVIDLLAPRDAWPRALSAIVASARDCDVVDIKL
                     ARTDANTRRLWQAGFLARDAPKPLNIMIPEGSRSADLYFDGAKWFFTLAESDQDTA"
     gene            complement(5159..5488)
                     /locus_tag="VFV99_03520"
     CDS             complement(5159..5488)
                     /locus_tag="VFV99_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058399.1"
                     /translation="MLVRIAAVVALLSACTGGASSTGIEPITCPPEGTNLTYANFGEE
                     LFADTCAMSGCHKSEKPVLTTHAAIKQNASAILDEAVYTDSMPRDRNMPLAQREMLGE
                     WLSCGAP"
BASE COUNT         1044 a         1800 c         1891 g          865 t
ORIGIN      
        1 gggcgcgctg atcggcgagc acgatttctc ggcgtttcgg gcggccggct gcactgccaa
       61 gaccacgatg cggcgcgtcg acgccatcgc acttacgcgc gctcagcccc acctgctgga
      121 catcgatatt cggggaaacg cgttcttgcg ccagatggtc cgcatcattg tcggcaccct
      181 tgccgaggtc gggacgggaa aacgctcggt ggctcaagta gcagagatcc ttgcgtccaa
      241 ggaccggacc caggccggaa ttaccgcgcc ggcgcacggc ctcgagctca tggaagtccg
      301 ctacgacggt gtgcgtccca ggcgggtgta agctcgcgcc tcgactcgcg ttgaggggat
      361 gtcggtggcc tccgaaccag ccacacaggt agagctcgaa gaagagcaac gccaggtcga
      421 ggaggcccaa gccggcaacc tcgacgccat gcgacctatc ctcgaacgtt acgcgcagcc
      481 gctctacggc acggtgatct tgccgcgcct gggcgatgcc gcgagcgccg aagaagttct
      541 gcgcgacacg ctcgcaacgg cggtcgagaa gattcgtcgg ttcacgtggc agggcaagtc
      601 gatctatccg tggctgcgcc agatcgcgat caacaaggtc tacgacgttc accggcaatc
      661 gaagcgctcg cggcgcttgg ccgacgcgat ggtgcacgag gttccgatcg agaccgatcc
      721 ggactcgcac gccgacgcgc agctgatcgc cgatcaagag cgcgccgcac atcgcgtgcg
      781 gatcgacgac gcgatggcac agatcgcgga gcgctatcgc gtcgcgatcg agctgcgact
      841 gatccaggag ctgtcgcgcg aagactgcgc gaagaagctc ggcatcacga tcggcacgtt
      901 cgacgtgctg ctgttccgcg cggtgcgcgc attccgcaaa cacttcggcg aacgcgtcga
      961 gggagaatcc gatggcgact gacgacgacg atatcctcac cggccccgtg cccgcgcccg
     1021 acgcggaagc gacgccgacc gagcgtgcgc aggcgaagac gttcgccgac atcgtcgaca
     1081 agacgctggc cggccgtacg ccgcccgcga tgagcgcgga ggatcgctcg ctgctcgagg
     1141 tcgccacggt cattcgcgcc gcgaccggca aggccgagct gtcggccacg aagcgtcgct
     1201 cgctcgtcga ggatgcgctc cgtcaggccg tcggaggtgc tggcactgcg gttgcgagcg
     1261 gcgtcacgcc gatctcgcgt gcgcgcaagc gctgggtgcc gtgggcgatc gcaggcgcga
     1321 gcacgctcgt cgccgcggcc gcgattctgc tgctgctact gcgcacgccg cacgtgccga
     1381 acacggcgcc gccggtggcg ccgccgccca tcgcggagaa ctggaagtcg cgcccggcgg
     1441 atccgctgat cgggccgatc agccgcgagc acgcgggtga tgcggcgacg cgcatcgatg
     1501 cgatttacgc cgaccggttg gacggctatc gcgagcgaac gctcgctcgc gggaggacgc
     1561 catgaagacg tacttggcag tgttggtcgc tgcggtgatc gcgatcacgg gctggatctc
     1621 gtgcggcagt gacaaggatc aaacgacgcc gacgacgaag acgcccgagc cgaccgatgt
     1681 catcggcaag gacctgatga tttcgttgtc gaaggcaaag aattttcatc acaaggctcg
     1741 cgtcttgatg agcgatggca agttcgctga agcgattgca tcggtccgcg agatcctgtc
     1801 gctccagttc ccgaagaacg cgccagaagc cgaagacgtt cgcaacgacg cccgcgcgtt
     1861 gctcgcgaag ctgctcgtcg gtcagggcca ggtcgaggaa gcgatgacgg tcgtcaccga
     1921 gggcatcgcc cagagccagc gcgagagctt cttcgtggca aacctataca cggtgaaggg
     1981 tgaggttcac gaagcgcgtg cggcattgct cgacgcaaac gatccgaaca ccaaggcgaa
     2041 gatcgtcgaa gagaagcgcg cggcgatcca ggcgtacgac aagtcgaacg agatcaacac
     2101 cgagctgcag aagacgctca tggaggagcg atgagggcgt cgacactcgg cgtcgtcctc
     2161 ttgacggcga tcatgatcgc gatcgcatgt ggtccgaagc agccgccgaa cggacccaag
     2221 caggacgaga tcaccgcact gtggatacag atccgggact ggcgccgcga ggcagggttg
     2281 ccggtcgatc cgcgtcgcga ggacgtgatt tcgatcgggc cgctgacgat cgaccaagcg
     2341 aagcacgtgt gcccggattc gcatccggtg ccgccgagct gcggtgacgt ctgcagcctt
     2401 gccgagaaca tctgcgacaa tgccgagtcg atatgcaacc tcgccgacga gctcggcaag
     2461 gacgacaagt gggcgcagga caagtgctcg aatgcaaaag cgtcgtgtcg tgacgccaag
     2521 aagcgctgct gtcagtgcag cgagaccgac agcaagtcag cgagcaaggc gtcgacgtgg
     2581 tgaaagcctg gccggcggcg ctgttctgcg cgctcgtggt cgcatgcgca gcggctccga
     2641 agccgatgca gaccgaagca ccgccgacca agaaccacga cggcggcgag ctgctgccgg
     2701 ctggcaagaa gcagcagatc gagcaactcg aagctgcgat cgcagccgat cgcgacaagc
     2761 tcgagctcgc cgagcccgcg caaccggact acatcaacac gcgtccagag cccatgggca
     2821 cgacaccggc ggtcgacaat ccgtcgtgca agccggcgaa gaccgacaaa tgcacgacga
     2881 gctgcacgct cagcaattcg atctgtacga acgccgactc gatctgccgg atcgcagtcg
     2941 agctcgatga cgactggtcg cgcggacgtt gcgcgaaggc gaacaagaca tgcgagggag
     3001 cgcgggacaa atgctgcggc tgtcagtaat cgcgttgttg gtcgtcggtg tgtcgtgtgg
     3061 cggtcgcaag atgggtgcga aagcgccagc gacgccctcc gagagcgcgc cagccgaggc
     3121 gggcgctgcg ccgtcgatcc tggcaccgag cacacacgac gagatcgaga agctcgacgc
     3181 cgagatcacg gcgagccggc aacaactgaa gctcgacgag ccgaccgagc aagcgatcca
     3241 ggcggcgccc acgcaggcgc tcggcgcgat gccgtcgacg caagacacca agtgtcatcc
     3301 ggcgcagaac gatacgtgca agacgagctg cacgctcagc gactcgatct gctccaacgc
     3361 gaacaagatc tgcaatctcg cgaacagcat gcccggcgac aactgggcga tgaacaagtg
     3421 cgcgaaggcg aacacgacgt gcgagtcgtc gaagacaaag tgctgcggct gtcagtagtc
     3481 gcaacgcgcc gcgtaggcat acgtatgcat tgacccgcgc ggcgagcctc tcgacgacag
     3541 gtggacgctg gtcgctcggg tgcgactacg cggaatgagt gagcggtctc cgcgagccga
     3601 gatggctcac tcgatgcacc gcgccgagcg gtgctgttct tccatgttgc ggaagcgggc
     3661 gtgttgggaa tcgcgaagaa cctccgatcg cgtctgggca acatgcgggc gtttggactg
     3721 cgctaccgtc gatgcgacgt gagctcggtg ctcgccgacg aaacggagat cgagatcgac
     3781 gagctgtggc ggcgatgcgc gaaaggctac ggcagcagcg ccgagcgtga tccgtggttg
     3841 catgcgagtc gcacggacca tccggggcat cacggcctcg tctacatact gcgttctacg
     3901 tatcacctcg atgccgtgat gatcgcgcgc cacgatccag tcgagtccgc catcgtcaat
     3961 tattgtggcc cggcgcagga ccgcgatctg atggccgagc tcggcgcgca tgtgatcgct
     4021 gacctggcgc gacggaagac cgtacggatt cgcgcagcca cgacgcacgc gccgatgatg
     4081 gacgcgctcg agtccattgg cttcgttcac tcgcggcgac cgggcgcggc cgtctccgca
     4141 aggatcgtgg cgtatggtcc gtgaagcgac gaaccgggca ccggagttca ccgtgcggct
     4201 cgcaagcgcc gcggacgggc cgacgatcgt agcggcagta cgcgagctgt tgccgggcgt
     4261 cgacgtcgcg cagcgccaca tgtggctcta tcgcgagaac ccgcatggcg aggcacttac
     4321 ctggatcgcg accgaggaag ctacgggcgc tgtcgctggc gttacctcgt tcttcccgcg
     4381 tcgcatcgtc gcgaacgggc gtgaggtcct ggcagctctc ggcggcgatg gctacgtgcg
     4441 tccggcgttc cggcgacgcg gactcgcatc ggcgatgcac ggcatgtccc gacgcgacat
     4501 gcagcgcttc gggatcgagg tgatgttcgg cacgccgatg cccgcgaacg ccacgccgct
     4561 tgcacgtcat ggcacgcaga acgtggtcga ggtcgtccgc tattcgcggt ggctcggcgg
     4621 ctggctcgcc ccgatcggcg cgttcgttcg ccgggcgttg cgagcgcacg ttcgcctcga
     4681 cgagttcagc gaacgcgatt ggcgcgtcga cgagctgtgg gagcgcacgc ggcccgagct
     4741 cggcattgcc acgattcgcg acgcccagtt ctacgactgg cggttccggc ggtcgccgtc
     4801 gcagcggcag cagccgtttg tcgtgctcga tcgcggacgc ccgatcgcgg cgtgcgcgct
     4861 cgagcgcgtg gggcgtcggt tgcgcgtgat cgacttgctc gctccgcgcg acgcgtggcc
     4921 ccgcgcactg agcgcgatcg tcgcctctgc gcgcgactgc gacgtcgtcg acatcaagct
     4981 cgcgcgcacc gacgcgaaca ctcgtcgcct gtggcaagcg ggtttcctgg cacgcgacgc
     5041 gccgaagccg ctcaacatca tgatccccga gggctcgcgc agcgcggacc tctacttcga
     5101 cggcgcgaag tggttcttca cgctcgccga gtcggaccaa gacacagcgt gaaccgcgct
     5161 acggcgcgcc gcacgacagc cactcgccga gcatctcgcg ctgggccagc ggcatgttgc
     5221 gatcgcgcgg catcgagtcg gtgtacacgg cctcgtcgag aatcgccgac gcgttctgct
     5281 tgatcgccgc gtgcgtcgtc agcaccggct tctcgctctt gtggcaaccg gacatcgcgc
     5341 acgtgtccgc gaacagctcc tcgccgaagt tcgcgtatgt gaggttcgtg ccctctggcg
     5401 ggcatgtaat gggttcgatt cccgtcgagc tcgctccgcc tgtgcaagcc gagagcaacg
     5461 cgacgactgc cgcaatgcgt accaacatca gaacaactcc tgtccgcgca ccctagcgcc
     5521 cgagctacga cctggcgagg tgagatcgcg ttggccgcat tcactgcgct ggcgtgcgcg
     5581 agaactacgc cgtcggcaat
//
LOCUS       DASUFX010000090         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1403090, whole genome shotgun sequence.
ACCESSION   DASUFX010000090 DASUFX010000000
VERSION     DASUFX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403090"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFV99_03525"
     CDS             complement(<1..275)
                     /locus_tag="VFV99_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058400.1"
                     /translation="MLTPGRDYLFFEKVDCTQQSLITTMTRSAVVITTNLIAVIPLES
                     TGSVLVATIATKHDGDALRFVKALLDDPAVDIEKLENALSSMYAGSHT"
     gene            394..1011
                     /locus_tag="VFV99_03530"
     CDS             394..1011
                     /locus_tag="VFV99_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058401.1"
                     /translation="MVTTVFLLSPARCSGERAGQLITSKRSELGKQLHAGRATVGEVM
                     AWLSALYFRGKLAYASRFGAAHPLPGALVMTPGFGLRPPEWLLSADDLRAMGSVDVET
                     PAFVRPLRRDAEQVISQYGSDARVVLLGSIATGKYLDTLLEVFGERLLFPETFVGRGD
                     MSRGGLMLRAARSGEELAYRPVVGAKLHGTRPPKLAKPAKLVGSP"
     gene            1011..1580
                     /locus_tag="VFV99_03535"
     CDS             1011..1580
                     /locus_tag="VFV99_03535"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX5058402.1"
                     /translation="MRRLIAFVLLAGCTDPSASKETTSVGACDGMLTVESDTSGQHVD
                     TGSEISWTNNPPTSGPHYPTWAAWDREYTDLPRGYYVHNAEHGGIVLLYRCDAGCPDV
                     VAQLLDTARRMAPDPLCQSPITKRVIVAPDPLLPDGVQVAAIAWNHAYTASCYDSYID
                     TFARENYAKAPENFCNDGTSLGNGTPIGP"
     gene            complement(1582..>2433)
                     /locus_tag="VFV99_03540"
     CDS             complement(1582..>2433)
                     /locus_tag="VFV99_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058403.1"
                     /translation="AVLDTAHDERTIDGQWRRACYLYPEQQTQRGQTHEPTKRAARPD
                     HSRHSRCPTLSPQKNSSGSVHTYERSKLLQQTSRGASPFDRPLDLGLTFGFVVAIASD
                     TTHLREVRTVLLVEDFAPLLRKWVRELQHSGKRVLAAANGDDALELIRDERPDVAVVD
                     LFLSTENGVEVIRKLKEQDTGLHAILVSAHLSVAYAIAGMRAGANDVCFKPGSIKHVV
                     DCVERGIDPEEADVEGTGPTLEQLEWEHISRVLLDCEGNITHAAERLGIYRQTLQRKL
                     RKYMPRS"
BASE COUNT          408 a          792 c          788 g          445 t
ORIGIN      
        1 gtgtgcgagc cggcgtacat cgagctcagc gcattctcga gcttctcgat atcgacggct
       61 ggatcgtcga gcagtgcctt cacgaagcga agcgcatcgc cgtcgtgctt cgtcgcgatg
      121 gtcgccacga gcacgctgcc ggtggactcg agcgggatca ccgcgatcag gttcgtggtg
      181 atgaccaccg cgctccgcgt catcgtcgtg atcagcgatt gctgcgtgca atcgaccttc
      241 tcgaagaaca gatagtcgcg tcctggcgtg agcacgcggg tgcaatcagc aaacgtcgtg
      301 cctcgtccag gccaagcgcg cacgcgcact tgcgcgccgg tgcacccacg tgcagctgcc
      361 agcctggcag gtgcggcgtg ctacaacgcg cgcgtggtga cgaccgtctt cttgctgagc
      421 cctgctcgtt gcagcggcga gcgagccggc cagctcatca cgtcgaagcg gtcggagctc
      481 ggaaagcagt tgcacgccgg tcgcgcgacg gtcggtgaag tgatggcgtg gctctccgcg
      541 ctctacttcc gcggcaagct cgcatacgcg tcgcgcttcg gtgccgcgca tccactgccg
      601 ggcgcgctcg tgatgacgcc gggcttcggc ttgcgtccac ccgagtggct gctgtccgcc
      661 gatgatctgc gcgcgatggg tagcgtcgat gtcgagacgc ccgcgttcgt gcggccactg
      721 cgccgcgatg ccgagcaggt catttctcag tacggcagcg atgcgcgcgt cgtgctcctc
      781 ggctcgatcg cgacaggcaa gtatctcgat acgctgctcg aagtcttcgg cgagcggctg
      841 ctgttccccg agacgttcgt cggccgtggc gacatgagtc gcggcggctt gatgttgcgt
      901 gcggcgcgca gcggcgaaga gctcgcgtat cgacccgtcg tcggcgcgaa gctgcacggc
      961 acgcggccac cgaagctggc aaagccggcc aagctggtag gctcgccgtg atgcgtcgcc
     1021 tgatcgcgtt cgtgctgctc gcgggttgca ccgacccatc ggcgtcaaag gagacgacga
     1081 gcgtcggcgc gtgcgatggc atgctcacgg tcgaatccga tacctcgggc cagcacgtcg
     1141 ataccggctc cgagatttcg tggacgaaca atccgccgac gagcgggccg cattatccga
     1201 cgtgggccgc gtgggatcgc gagtacacgg acctcccgcg cggctactac gttcacaacg
     1261 cggagcacgg cggcatcgtg ctgctctacc gctgcgacgc gggctgtccc gacgttgtcg
     1321 cgcagctgct cgataccgcg cgccgcatgg ctcccgatcc actgtgccaa agcccgatca
     1381 cgaagcgcgt gatcgtcgcg cccgatccgc tgctgcccga cggcgtccag gttgctgcaa
     1441 tcgcgtggaa ccacgcgtac accgcgagct gctacgactc gtacatcgat acgttcgcgc
     1501 gcgaaaacta cgcgaaggcg cccgagaact tctgtaacga tggcacgagc ctcggcaacg
     1561 gtacgccgat cgggccgtag ctcacgaccg cggcatgtac ttgcgcagct tgcgctgcag
     1621 cgtctggcga tagatgccga gtcgctccgc cgcgtgggtg atgttgcctt cgcaatcgag
     1681 caacacgcgg ctgatgtgct cccactcgag ctgttcgagg gtcggaccgg tcccctcgac
     1741 gtccgcctct tccggatcga tgccacgctc gacgcaatcg acgacgtgct tgatcgagcc
     1801 gggcttgaag cagacgtcgt tcgctcctgc gcgcataccg gcgattgcgt acgcgacgct
     1861 caagtgagca ctgaccagaa tcgcgtgcag gccggtgtcc tgttccttga gcttgcggat
     1921 gacctccacg ccgttctcgg tcgacaagaa caagtcgacg actgcaacat cagggcgctc
     1981 atcgcgaatg agctccagtg cgtcatcgcc attcgccgcg gccagcacgc gcttgcccga
     2041 gtgctgcagc tcgcgaaccc atttccgcaa aagcggagca aagtcttcga ccagcagaac
     2101 ggtacggacc tcgcgcaggt gcgttgtatc ggacgcgatc gccaccacga aaccaaacgt
     2161 tagccccaaa tccagaggac ggtcaaaggg ggaagcccct cgcgaggtct gctgcaacaa
     2221 tttgctgcgc tcataggtgt gcacactccc actggaattt ttctgtggcg acaatgtggg
     2281 acaccgggag tgcctggaat gatcaggcct cgcggcgcgt tttgtcggct catgcgtctg
     2341 cccgcgttgt gtctgttgct cggggtacag gtagcaggcg cgacgccact gaccgtcgat
     2401 cgtgcgttcg tcgtgggccg tgtcgagaac ggc
//
LOCUS       DASUFX010000091        13322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1264308, whole genome shotgun sequence.
ACCESSION   DASUFX010000091 DASUFX010000000
VERSION     DASUFX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13322
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264308"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="VFV99_03545"
     CDS             <1..1671
                     /locus_tag="VFV99_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058404.1"
                     /translation="KLTVTNLDTGAKVASTVGPRATVSVPGGDGSEHTFKIRVENHST
                     ATLKAKLRAVGHGGAGVTPALLAAARANLDRIAKEIDYTHLKNYGLSGSLTDQFMSAL
                     SAEYETQHRDQYIARVKALASMAFFALPEVLPPADGTSTPFHGLDMNQFDTLMNIEDQ
                     VFNRLVGLNNNDTNGVRPFSVCETRFIIETYVRPHVAFPGFDPYKAAYTTYAASCPQK
                     DKDEWYNFRGLGGLRPSWVESNLADRFLRRMAKTCQNPTSAWTAECGNWNADRLGYRQ
                     QKNKELAARTMFYAPADEAYLINPSNSLVLLEDRNGDGVGEFLRPGAIKLKNGDTGTL
                     QVNATSQFAGTLKFQPTSGSLRTVSPGEVVAEDAVDSRWQPSLMSRPDLGLMQVFSSS
                     TGCTAASLDPAQCPLMRRFYSMIDRHENFYQTFSALQPTYYGISSQPSPLVACSITLA
                     ASHQWDSAGTPPGGTAGFIFLMRIPFKDILTGNETSVSTLMPGPKTTSLQSLYAGTGH
                     LDFSSLWLDVASLSNNQYETEHEISAFGAVRADQIEGILVVRKPAAVP"
     gene            complement(1755..2972)
                     /locus_tag="VFV99_03550"
     CDS             complement(1755..2972)
                     /locus_tag="VFV99_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058405.1"
                     /translation="MRWLGIVLVAACGAKAPPKVENAQFTAPAPNNLTYRPLTIAMRR
                     PAIDLTVVMPEFHEELRWPLSGMNHPALVPRFAVAAELAVPGVEWEELCARGVQNRTS
                     GAQKELLAYLRGWCAVQKRDIDAACAHLRPLLGSVMAGMTAAVRQDLANILVEQGDSE
                     KAEHWLSKHDIRDMRTLDLLAANYIEVGSQADAFAINRRAMDSDDHATPATKCYRLVR
                     RIVIGLDVEPMLAVEALKELGLKAEIPDPICKRLWNKILCWQDHDRCAEYFNDENVDA
                     RERLLVDAYYAWPSGAVSAAEWLTYADKAELAIPVPGASELVVSALETAMRIIGDCQT
                     GHAADINSTIATVRADPNSAMYEARLQKLEHDCPRPPQWMPSTLPGAVAVPPPESTSP
                     PNSVTNGKLPTVP"
     gene            3256..4095
                     /locus_tag="VFV99_03555"
     CDS             3256..4095
                     /locus_tag="VFV99_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058406.1"
                     /translation="MLLTLPAAACIEPSPDITAVDQYDWAQYEGQRDTRMVQYTGTWS
                     SSCQYNTRFGCGSALVHVSIRVRPAEHADLAWKRVGVVYHTPDDRTERTAVGSYSKTL
                     TDGDEEWTVSFYAPSSDAIVVFDAWYQDGAGKTWIDDNAGELHVVNVGPAYQVVRVEP
                     WLNTVTVGANGVTGNISLQLADLDYDKQLELVATKDHWQTTLHFGMGEANEKNKLHWV
                     EDFPYSPGRERWQIELDVPGGSEHFEYALVYRHGVVNGARTYEFWDNNGGGNYRVEAA
                     ILQ"
     gene            4092..4940
                     /locus_tag="VFV99_03560"
     CDS             4092..4940
                     /locus_tag="VFV99_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5058407.1"
                     /translation="MTETIATDLHGHTRFSDGRAEPEDYVAFRAGLGMQVIAVSDHDT
                     FAGVPRAAEAARAHGVTLVPAMEVTSFIHFGTPRAEQIHVLAYFPPERALDGSLADTF
                     LGARAVAVSRKWRELCLGWLTGLPEEQRYFIDPERSLAQHRGTEFPQLQDFLNLVYDR
                     NRLVYQDFIRYHVQFWDNDADLFGWQPEEAIEAIRSDGALDIVAHPVRVRDKTRMDRV
                     IDEASGIEVYTSRHRHDIAAGFRERAEAAGKHWTASADDHGHVKYVRPPDGTPRRTIE
                     LILTGR"
     gene            4937..8002
                     /locus_tag="VFV99_03565"
     CDS             4937..8002
                     /locus_tag="VFV99_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEX5058408.1"
                     /translation="MSERVIDEGGVLVAVTARLDRPVSALELCGELPCWQQPIAMQRT
                     GSAWQAHVRARPGVYEIKLREPSGTWSVDPEWRTVARDGEHNGVLVIGGSDEPVLHAP
                     ASPWLRVLDDGRIAVRAALRHGHRDRLALHVDDGAGMRTCPMRETGRDGSHRWFEIEL
                     AGAGRTLEYAFVLADGRVIASSTGAFRIDPHDLVPHVPPWWRDAVVYTIFVDRFRRGG
                     SAGGWRDPQAWAREHRAGGDLAGVREALPYLADLGVTALHLTPVCVAPSVHRYDAIEP
                     TSIDPELGGDVAFARLVDEAHARGLRILVDVATTHVDRDFAPFRDVRAHGPASRYWHW
                     FRGHRWPFIDGPDPGYDHYQKGQWREPLLALDEPEVRDLIVSWFDTWARRGVDGMRVD
                     AAADLPLPLLARVRSAVRAVRPDAIVFGEVVPSCLDRFAPHVLDAATDFAHREAMLGW
                     LGGESATRWASAAAALRRRGAATEVALGFAGTHDQPRIATVTGDAALARLGLVAIALG
                     ARIPLLYYGDEVGLVATAVREFEDSWPDRQPMPWQEAAWDHATLDAVRAALALRRDHT
                     LLRRGDEELRAIDDDTVLIRRHRLGDAIDIVIHRGTSPQTVELPPGAARVLFALGDVE
                     LAGAQLRLGARALAVIDRREVLDHDLVAHNATIAAEAFAGGRAECPAYPTRLYVTVTE
                     ACNLRCAHCITDAPARTRSGRARTLQPWLLDALDEAFAHADYVAFTHGGESLAAPIFP
                     HVLGRLARARGRSPGRADVHLASNGMLLDADRVAMLIDRGVTSVMVSLDGAMPATNDR
                     IRVLGRFERVVANIAAAVDARSRRGADLRIGISTVVGASNVRELPALGRLCLDLGVDW
                     LKLEETYPATSFARVDLLAADDPAVLAAMSALRDVVAGYSLVLVEHIAPPTECTCSGD
                     AAARAFRTADDFANRATFRPCRAAWEQAAIDPDGTVHVVDYAGSRLGSLLDAPMLALW
                     NAPAALAARDRALAASSVVRRRVCIAHDPLITKIPHLATSLRHEPQHRR"
     gene            complement(7973..8431)
                     /locus_tag="VFV99_03570"
     CDS             complement(7973..8431)
                     /locus_tag="VFV99_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5058409.1"
                     /translation="MVADHVEVAQRYIIELLEERSLDVLSALVVDDYVLREPLALLEG
                     RDALSERLCDTAFSDIIIVIEDLIAADDRVVVRSTWQGVHRGTFFGVEATGQRVTLDV
                     VQVLTFVGSRVSEDCTYYDAYALFEQIGALPPVDKLAKPVRLAPVLRLVP"
     gene            complement(8438..9775)
                     /locus_tag="VFV99_03575"
     CDS             complement(8438..9775)
                     /locus_tag="VFV99_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5058410.1"
                     /translation="MLRWPGNFLHFIFLLLVLILCGVVYGLARLGIALFIWGPRRARA
                     VGTLRGWILKQAMSTLGATFIKLGQVMSTRPDLFAPEVIEQLRTLQDRIPPFAFRKVK
                     RILEEDFGTSIAELFSELDETPVAAASVAQVHRARLRDGSEVAVKVLRPGVRRQVERD
                     QVILIWFARVISIHPKWRRNDPVGHTRHFIEAIHDQTDLAIEQANYERFHANFKGTPR
                     VRFPVVHKELCSKRVLTMEFIRGTKVDALPPGNYPKLAEVVRTTFFKMCFEDGFVHAD
                     LHPGNMVVQDNDSIVVYDVGLAKLLHEDVLIQFIDMTKCIAMGTPEDIVAHLKRFHTY
                     IGEIDWEALRVEVTAFAAKFRQKDAAKLEYGELIGEMFAIGRKHRVRPVTDMMLVFVA
                     LITAQGIGKMLEPGHNVFGAVAMYLIPILQKRNEKVPDTDEARAARGGMTAAV"
     gene            9829..10434
                     /locus_tag="VFV99_03580"
     CDS             9829..10434
                     /locus_tag="VFV99_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5058411.1"
                     /translation="MIAAFRGFAKAHFLEACAVMNTFKADFEEIRRRAREHMEQGSVT
                     EAYKADVDKVVSVLNEVLATEIVCTLRYKAHYYMAQGIHSQAIAEEFLQHSNEEQQHA
                     DMVAKRITELNGKPNFNPDGLHTRSHAQYSVGAKLEDMIREDLVAERIAIGTYSEIVR
                     WLGTDDPTTRRMMEQILATEEEHADDMAKLLARIGAKSDGH"
     gene            10487..12022
                     /locus_tag="VFV99_03585"
     CDS             10487..12022
                     /locus_tag="VFV99_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058412.1"
                     /translation="MYGVLSDEEIIHGLADDLPVGLFVARAPGGELVYVNRMFAEIMG
                     QSGREDASAGSYSEPYGILTRDGKPYPEEKLPFVLALEEKRVVVVDDIMVRRPDRSVV
                     HVRAFANPVMKDGEVTHVVICFFDISEEIVAKLAHEESERRLYRSQRLEAIGTLAGGI
                     AHDFNNLIFGIKLLAAELSANEHDPQRRASLRMIDDITERSAMLTKSLLGFAGRGNHR
                     AVPVALNDVIVGMRELLARTLGGIQVIFQLDATQRGTVIGDRAQLEQVVMNLVVNARD
                     ALKEKGGRVVVKTKSGAGTVMLEVLDDGPGIPVELRERIFEPYFTTKTSGPQRGTGLG
                     LATVFGIVERHKGAIEAGPGLHGAGAAMRVTLPAAPEAADEEPAVDDLGLEPGSGTIL
                     VVDDDLMVRRAVSMTLRGLGYTTFEAASGLEAVDLYRQHHAIIRAVVLDVIMPGMSGR
                     ATYAAMRAVDPTVAVLLMSGYTKNEDVQVLLEQGARGFVMKPYSVEVLARSLADALKQ
                     PNA"
     gene            complement(12023..12880)
                     /locus_tag="VFV99_03590"
     CDS             complement(12023..12880)
                     /locus_tag="VFV99_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5058413.1"
                     /translation="MQTLPGNQEAIEAWNTVLFDKFVAYRPMVTTALAQHGNRAIERL
                     GLKAGMKVVDIGCGFGDTTQVLGQQVGPSGRAVGLDAASRFIETARTEATGLEQVHFE
                     VADIEAGVPGGPYDAAFSRMGTMFFNQPVIALRNIRKALAPGAPLRMVVWRKKETNEA
                     FYNAELIVRDILGDPPKNDQVTCGPGPFSMASPDLVSDQMKGAGWTDITFERSDADLF
                     FGSTVEQAIDFALTLGPAGEVVRLAGAEGVRRRDEIVTALKSAIEPMMRGDGCYAPSS
                     CWIVGARNQ"
     gene            12921..>13322
                     /locus_tag="VFV99_03595"
     CDS             12921..>13322
                     /locus_tag="VFV99_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEX5058414.1"
                     /translation="MVPDAWRHILEREVAVYRKLDVRQRAKLVEDLLVFAGKEFVGLD
                     GFVVDDRVRVHVSATAALMVLGIGIDRFAHVRRIEMREARYLDERGNVAEGHYEYATH
                     ESIGPIGILHLAWKGMLDGAARNDGAHVGIHE"
BASE COUNT         2244 a         4477 c         4340 g         2261 t
ORIGIN      
        1 aagctcacgg tcacgaacct cgacaccggc gcgaaggtcg cgagcacggt cggtccgcgc
       61 gcgactgtct cggtacccgg cggagacggt agcgagcaca cgttcaagat tcgcgtcgag
      121 aaccactcga ccgccacact caaggccaag ctccgcgcgg tcggtcacgg cggcgccggc
      181 gtcacgcccg cgttactcgc cgcagcacgc gcgaacctcg atcgcatcgc gaaagagatc
      241 gactacacgc acctcaagaa ctacggcctc tcgggctcgc tcactgatca gttcatgagc
      301 gcgctctcgg ccgagtacga aacgcagcat cgcgaccagt acatcgcgcg cgtcaaggca
      361 ctcgcctcga tggcgttctt cgcgctgccc gaggtactgc cgcccgccga cggtacgtcg
      421 acgccgttcc acggcctcga catgaaccag ttcgacacgc tgatgaacat cgaagaccag
      481 gtcttcaacc gcctcgtcgg cctcaacaat aacgacacga acggcgtccg tccgttctcg
      541 gtctgcgaga cgcgcttcat catcgagacg tacgtgcggc cgcacgtcgc gttcccgggc
      601 ttcgatccat acaaggccgc gtacacgacc tacgccgcga gctgcccgca gaaggacaaa
      661 gacgagtggt acaacttccg cggcctcggc ggtctgcgcc cgagctgggt cgagtcgaac
      721 ctcgccgacc gcttccttcg ccgcatggcg aagacgtgcc agaacccgac gtcggcgtgg
      781 accgccgaat gcggcaactg gaacgccgac cgtctcggct accgccagca aaaaaacaag
      841 gagctcgcgg cgcgcacgat gttctacgcg cctgccgacg aggcctacct gatcaatccg
      901 agcaactcgc tcgtcctcct cgaagaccgc aacggtgacg gcgtgggcga gttcctccgc
      961 cccggcgcga tcaagctcaa gaacggcgac acgggtacgc tccaggtcaa cgcgacgagc
     1021 cagttcgccg gcacgctcaa gttccagccc acgagcggct cgctccgcac cgtcagcccg
     1081 ggcgaagtcg tcgccgaaga cgctgtcgac tcgcgctggc agccgtcact gatgagccgc
     1141 ccagacctcg gtctcatgca ggtgttctcg tcgagcaccg gctgcacggc cgcctcgctc
     1201 gatcccgcgc agtgcccgct gatgcgtcgc ttctactcga tgatcgatcg tcacgagaac
     1261 ttctaccaaa cgttctcggc gctgcagccg acctactacg gcatctcgag ccaaccgtcg
     1321 ccgctcgtcg cgtgctcgat cacgctcgcg gcgtcgcacc agtgggacag cgcgggcacg
     1381 ccgccgggcg gcaccgcggg cttcatcttc ctgatgcgca tcccgttcaa ggacatcctg
     1441 accggcaacg agacgagcgt ctcgacgctg atgcccggcc ccaagaccac cagcctgcag
     1501 agcctctacg ccggcaccgg tcacctcgac ttctcctcgc tgtggctcga tgtcgcgagc
     1561 ctgtcgaaca accagtacga gaccgagcac gagatcagcg cattcggcgc cgttcgcgcg
     1621 gatcagatcg agggaatcct cgtcgtccgc aagccggcgg cggtgcccta acgagctttt
     1681 tgtctgctac cggcggtcac ggcaacgcgt tcgtctgcag acgctgtgac cgccggtagc
     1741 gggcgccttg tgcgctacgg caccgtcggc agcttgccgt ttgtcacgga gttcggcggt
     1801 gacgtcgatt ccggcggtgg caccgccacg gctccgggca gagtcgatgg catccactgt
     1861 ggcggtctcg gacagtcatg ttcgagcttc tgcaaacgcg cttcgtacat ggcgctgttc
     1921 gggtcggccc gcaccgtcgc gatcgtgctg ttgatgtccg cggcatggcc cgtctggcag
     1981 tcgccaatga tgcgcatggc cgtttcgagc gcactgacaa cgagctccga cgcacctggt
     2041 actggaatcg ctagctctgc tttgtccgca tacgtaagcc actcggcagc gctcacagcc
     2101 ccggacggcc aggcatagta cgcatcgaca agcagtcgct cgcgcgcatc gacattctcg
     2161 tcgttgaagt actcggcgca gcggtcgtgg tcctgccagc acaagatctt gttccacaga
     2221 cgcttgcata tcgggtcggg gatctccgcc ttgaggccga gctctttgag cgcctcgacg
     2281 gcgagcatcg gctcgacatc gagcccgatc acgatccggc gcacgagtcg ataacacttc
     2341 gttgccggtg tcgcgtgatc atcggaatcc atcgcgcggc gattgatcgc gaacgcatct
     2401 gcctgcgatc cgacctcgat gtagttcgcc gcaagaagat cgagcgtccg catgtctcgg
     2461 atgtcgtgct tgctgagcca gtgctcggcc ttctccgagt ccccttgctc gacgaggatg
     2521 ttcgcaaggt cctgacgcac cgctgccgtc atgccggcca ttacggaacc gagcagcggc
     2581 ctcaggtgcg cgcatgccgc atcgatgtcg cgcttctgca ctgcgcacca gccacgcaga
     2641 tatgcgagca gttccttttg cgcacccgac gtacggttct gcacgccacg cgcgcagagc
     2701 tcctcccatt cgacgcccgg caccgcgagc tcagcggcca cggcgaatcg cggaacaagt
     2761 gccggatgat tcatgcccga gagcggccag cgcagctctt cgtgaaactc gggcatgacc
     2821 acggtgagat cgatcgcggg tcgccgcatg gcgatcgtca acggtcggta cgtaaggttg
     2881 ttcggcgccg gcgctgtgaa ctgcgcgttc tcgacctttg gcggcgcctt cgcgccgcac
     2941 gcagcgacga gcacgatccc cagccaacgc acgacctgat gctcgcacgg cgcccgtcga
     3001 ggatcgtcac gattctgtga gcgccgcaag cagcagccgc tctagcgatc ggaccgctgt
     3061 ggcagctcta aacgccacgg ttgcgatctg cgacatcgcc agccggatcg cacgcgcaag
     3121 ccgcggattc tcgcggctgg ccggtggcat gtcggttgct gttagacgcg ggccaaaatg
     3181 cccgaatcat cttctagact tttcgctgcc gcgctcgccc ggctgcgtcg ccgcggcgca
     3241 gcgctcggta gcggcgtgct cctgaccctg ccggcggcgg cgtgtatcga gccgtcgcca
     3301 gatatcaccg ccgtcgatca gtacgattgg gcgcagtatg aaggccagcg cgacacgcgc
     3361 atggtccagt acaccggcac gtggagctcg agctgccagt acaacacgcg cttcggttgc
     3421 gggtcggcgc tcgtgcacgt gagcatccgc gtgcgtccgg ccgagcacgc ggatcttgcg
     3481 tggaagcgcg tcggcgtcgt gtatcacacg cccgacgatc gcaccgagcg cacggcggtc
     3541 ggcagctact cgaagacgct gacggacggt gacgaggaat ggacggtgtc gttttacgcg
     3601 ccgagctctg acgcgatcgt cgtgttcgac gcctggtatc aagacggtgc gggcaagacg
     3661 tggatcgacg acaacgcagg tgagctccac gtggtcaatg tcggtcccgc gtatcaggtc
     3721 gtgcgcgtcg agccgtggct caacaccgtc acggtcggcg ccaacggtgt gaccgggaac
     3781 atctcgctgc agctcgcgga tctcgactac gacaaacagc tcgagctcgt cgcaacgaag
     3841 gaccactggc agacgacgct gcatttcggt atgggcgagg cgaacgagaa gaacaagctg
     3901 cactgggtcg aggacttccc gtactcgccc ggtcgcgagc gctggcagat cgagctcgac
     3961 gtgcccggcg gctcggagca tttcgagtac gcgctcgtgt atcgccatgg cgtcgtcaac
     4021 ggagcgcgca cgtacgagtt ctgggacaac aacggcggcg gtaactatcg cgtcgaggcg
     4081 gcgatcctcc agtgaccgag acgatcgcga cggatctgca cggccacacc cgattctccg
     4141 acgggcgtgc cgagcccgag gactatgtcg cgtttcgcgc cggcctcggt atgcaggtga
     4201 tcgcggtgtc cgaccacgac acgttcgccg gcgtgccgcg cgcggcggag gcggctcgcg
     4261 cgcacggcgt cacgctcgtg ccggcgatgg aagtcacctc gttcattcac ttcggcacac
     4321 cgcgcgccga gcagatccat gtgctcgcgt attttccacc cgaacgcgcg ctcgacggct
     4381 cgctcgccga cacgttcctc ggcgcgcgcg ccgtcgccgt gagccgcaag tggcgcgagc
     4441 tgtgtctcgg ctggctgacc ggcttgcccg aagagcagcg ctacttcatc gatcccgagc
     4501 gctcgctcgc ccagcatcgg ggcaccgagt tcccgcagct gcaggacttc ctgaatctgg
     4561 tctacgaccg caatcggctc gtctaccagg acttcatccg gtaccacgtg cagttctggg
     4621 acaacgatgc cgacctgttc ggctggcagc cggaggaagc gatcgaggcg atccgcagcg
     4681 acggcgcgct cgacatcgtc gcgcacccgg tgcgcgttcg cgacaagacc cgcatggatc
     4741 gcgtgatcga cgaagcgagt ggcatcgagg tgtacacgtc gcggcaccgc cacgacatcg
     4801 ccgccggctt tcgcgagcgc gccgaggccg ccggaaaaca ctggaccgcg tcggccgacg
     4861 atcacggcca cgtgaagtac gtgcggccac ccgatggcac gccgcgccgc acgatcgagc
     4921 tcatcctcac gggccgatga gcgagcgcgt gatcgacgag ggcggggtgc tggtcgcagt
     4981 gactgcgcga ctcgaccggc cggtcagcgc actcgagctg tgcggcgagc tgccgtgctg
     5041 gcaacagccg atcgcgatgc agcgcaccgg cagcgcgtgg caagcgcacg ttcgcgcgcg
     5101 ccccggcgta tacgagatca agctgcgcga accgtcgggc acgtggtctg tcgatcccga
     5161 atggcgaacg gtcgcccgcg acggcgagca caatggcgtg ctcgtgattg gcggcagcga
     5221 cgagcccgtt ctgcacgcgc cggcctcgcc gtggctgcgc gtgctcgacg atggccgcat
     5281 tgccgtgcgc gcggcgctgc gtcatggtca tcgcgatcgc ctcgcgttgc acgtcgacga
     5341 tggtgccggc atgcgtacgt gtccgatgcg cgagaccgga cgcgacggct cgcatcgctg
     5401 gttcgagatc gagctcgcgg gcgcagggcg cacgctggag tatgcgttcg tgctcgcaga
     5461 cggtcgcgtg atcgcgtcga gcacgggcgc gttccgcatc gacccgcacg acctcgtgcc
     5521 gcacgtgcca ccgtggtggc gagacgcggt cgtctacacg atcttcgtcg accgatttcg
     5581 tcgcggcggt tcggccggcg gctggcgcga tccgcaggcg tgggcacgtg agcaccgcgc
     5641 gggtggcgat ctcgccggcg tgcgcgaagc attgccgtat ctggccgacc tcggcgtgac
     5701 cgcactgcat ctcacgccgg tgtgcgttgc gccctcggtg caccgctacg acgcgatcga
     5761 gccgacatcg atcgatcccg agctcggcgg cgacgtcgca ttcgcgcgtc tcgtcgacga
     5821 agcgcatgca cgcggcctcc gcatcctcgt cgatgtcgca accacgcacg tcgatcgcga
     5881 cttcgcgccg ttccgggacg ttcgcgcaca cggcccggcg tcgcggtact ggcactggtt
     5941 tcgcggacac cgctggccgt tcatcgacgg acccgatccc ggctacgacc actatcagaa
     6001 gggacagtgg cgggagccgc tgctcgcgct cgacgaaccc gaagtgcgcg acctcatcgt
     6061 gtcgtggttc gacacctggg cgcggcgcgg tgtcgacggc atgcgcgtcg atgcggcggc
     6121 ggacttaccg ctgccgctgc tcgcgcgcgt acgcagcgct gtgcgggccg tgcgtcccga
     6181 tgcgatcgtg tttggtgagg tcgttccctc gtgcctcgat cgattcgccc cgcatgtgct
     6241 cgacgctgcg acggacttcg cgcaccgcga ggcgatgctc ggctggctcg gcggcgagtc
     6301 agcaacgcgg tgggcgagcg ccgcggcagc actccggcga cgcggtgctg cgaccgaggt
     6361 cgcgcttggc ttcgccggca cccacgacca gccgcgcatc gcgacggtta ccggtgatgc
     6421 cgcgctcgcc cggctcggac tcgtcgcgat tgcactcggt gcgcgcatcc cgctgctcta
     6481 ttacggcgac gaggtcggtc ttgtcgcgac cgcggtccgc gagttcgaag actcgtggcc
     6541 cgatcggcag ccgatgccgt ggcaggaagc agcatgggat cacgcgacgc tcgacgccgt
     6601 tcgcgcggca ctcgcacttc gtcgcgacca cacactcctg cgccgcggcg acgaggagct
     6661 gcgcgctatc gatgacgaca cggttctcat tcgccgccac cgcctcggcg acgcgatcga
     6721 tatcgtcatc catcgcggta cttcgccgca aacggtcgag ctgccgcccg gcgccgcgcg
     6781 cgtgttgttc gcgctcggcg acgtcgagct tgcaggcgcg cagcttcgac tcggtgctcg
     6841 cgcgctcgcc gtgatcgatc gccgcgaggt gctcgaccac gatctcgtcg cgcacaacgc
     6901 cacgatcgcg gccgaagcgt tcgccggcgg tcgtgccgag tgcccggcgt acccgacgcg
     6961 gctctatgtg accgtcaccg aggcgtgcaa cctgcgttgc gcgcactgca tcaccgatgc
     7021 acctgcacgc acgcgctccg gccgcgcgcg cacgctgcag ccatggttgc tcgacgcgct
     7081 cgacgaggcg ttcgcgcacg cggattacgt cgcgttcacg cacggcggtg agtcgcttgc
     7141 cgcgccgatc ttcccgcacg tactcggccg cctcgcacgc gcgcgcggtc gctctcccgg
     7201 acgcgcggac gtccacctcg ccagcaatgg catgctgctc gatgccgatc gcgtcgcgat
     7261 gttgatcgat cggggcgtca cctcggtcat ggtctcgctc gacggcgcga tgcccgcgac
     7321 caacgatcgc attcgcgtgc tcggccgctt cgagcgcgtc gttgcgaaca ttgccgccgc
     7381 agtagatgcg cgctcgcgtc gcggcgccga cctccggatc ggaatctcga ctgtcgttgg
     7441 cgcgagcaac gtccgcgagt tgcccgcgct ggggcggctc tgtctcgacc tcggtgtcga
     7501 ttggctcaag ctcgaagaga cgtacccggc aacgtcgttc gcacgcgtcg acctcttggc
     7561 agcagacgat cccgcagtgc tcgccgcgat gtcggcgctt cgcgatgtgg tcgcgggcta
     7621 ctcgctcgtg ctcgtggaac acatcgcgcc gcccaccgag tgcacgtgta gcggtgacgc
     7681 cgccgcacgt gcgttccgca ccgccgacga cttcgcgaat cgcgcgacgt tccggccgtg
     7741 tcgcgccgcc tgggaacagg ccgcgatcga tccggacggc accgtgcatg tggtcgacta
     7801 cgctggctcg cgactcggct cgcttctcga cgcgccgatg cttgcgctgt ggaatgcacc
     7861 tgcggcgctc gcggcccgcg atcgtgcgct ggccgcttca tcggttgtgc gtcgccgcgt
     7921 gtgcatcgct cacgatccgc tgatcacgaa gatcccgcac ctcgcgacga gcctacggca
     7981 cgagccgcag caccggcgct agccgcaccg gcttcgcgag cttatcgacg ggaggcagcg
     8041 cgccgatctg ctcgaacagc gcataggcgt cgtagtacgt acagtcctcg ctgacacggc
     8101 tgcccacgaa cgtcagcacc tgcacgacat cgagcgtgac gcgctggccc gttgcctcga
     8161 cgccgaagaa cgtaccgcgg tggacacctt gccatgtcga ccgcacgacg acgcgatcgt
     8221 ccgcggcgat caggtcttcg atcacgatga tgatgtcgct gaacgcggtg tcgcagaggc
     8281 gctcgctgag tgcatctcgg ccttcgagca aggcaagcgg ttcgcgcagc acgtaatcgt
     8341 ctacgacgag cgccgacagg acatcgagag agcgttcttc gagaagctcg atgatgtaac
     8401 gctgcgcaac ctcgacatgg tcggcgacca tgcctcgtca gactgcagcg gtcatgccac
     8461 cgcgcgcggc gcgcgcttca tccgtgtcag gcaccttctc gttccgcttc tgcaagatcg
     8521 ggatcaggta catcgcgacc gcgccgaaca cgttgtggcc gggctcgagc atcttgccga
     8581 tgccttgcgc tgtgatcagc gcgacgaaca cgagcatcat gtccgtgaca ggacgtacgc
     8641 ggtgtttgcg gccgatggcg aacatctcgc cgatgagctc gccgtattcg agcttggcgg
     8701 cgtccttttg gcggaacttc gcggcgaacg cggtcacttc gacacgcagc gcttcccagt
     8761 cgatctcgcc gatgtacgtg tggaatcgct tgaggtgcgc cacgatgtcc tcgggcgtgc
     8821 ccatcgcgat gcatttcgtc atgtcgatga actggatgag gacgtcctcg tgcagcagct
     8881 tcgcgaggcc gacgtcatag accacgatgc tgtcgttgtc ttgcacgacc atgttgccgg
     8941 gatggaggtc ggcgtggacg aagccatcct cgaagcacat cttgaagaac gtcgtgcgca
     9001 cgacctcggc gagcttggga tagttgccgg gcgggagcgc gtcgaccttc gtaccgcgga
     9061 tgaactccat cgtgagcacg cgcttggagc acagctcctt gtgcacgacc gggaagcgga
     9121 ccctcggcgt gcctttgaag ttcgcgtgaa agcgctcgta gttcgcctgc tcgatcgcga
     9181 ggtcggtttg atcgtggatc gcttcgatga agtggcgcgt gtggccgacg ggatcgttgc
     9241 ggcgccactt gggatggatc gagatcacac gcgcgaacca gatcaggatc acttgatcgc
     9301 gctcgacctg gcggcgcacg ccgggacgca gcaccttgac cgcgacctcg ctgccgtcgc
     9361 ggaggcgcgc tcggtgcacc tgtgccacgc tcgcagcggc gaccggcgtt tcgtcgagct
     9421 cgctgaacag ctcggcgatc gacgtgccga agtcctcttc gaggatgcgc ttgaccttgc
     9481 ggaacgcgaa cggcgggatt cgatcctgga gcgtgcgcag ctgctcgatc acttcgggtg
     9541 cgaagagatc ggggcgcgta ctcatgacct gaccgagctt gatgaacgtc gcgccgagcg
     9601 tgctcatcgc ttgcttgagg atccagccgc gcagtgtgcc taccgcacga gcgcgacgtg
     9661 gtccccagat gaacagcgcg atgccgagtc gcgcgaggcc gtacaccacg ccgcacaaga
     9721 tcaggacgag gagcaggaaa atgaagtgga ggaagttccc gggccagcga agcactgcgg
     9781 gcccatccta accgcggcga ccgcgcacgt gtgtcactct cgcgcgctgt gattgcggcc
     9841 tttcgaggct ttgcaaaggc gcatttcttg gaagcctgcg cggttatgaa caccttcaag
     9901 gcagacttcg aggagattcg gcgccgcgca cgcgagcata tggagcaggg ctcggtgacc
     9961 gaggcctata aggccgacgt cgacaaagtc gtgagcgtgc tcaacgaggt gctggcgacc
    10021 gagatcgtct gcacgctgcg ctacaaggcg cactactaca tggcccaggg catccacagc
    10081 caggcgatcg cagaggagtt cctccagcac tcgaacgagg aacaacagca cgccgacatg
    10141 gtggcaaaac ggattaccga gctaaacggt aagccaaact tcaatcccga cggattgcat
    10201 acgcgcagtc acgctcaata cagtgtcggc gcgaagctcg aagacatgat tcgtgaggac
    10261 ctcgttgcgg aacgcatcgc cattgggacg tattcagaaa ttgtccggtg gctcggcacg
    10321 gacgatccaa cgacacgccg catgatggaa cagatcttgg cgaccgaaga agagcacgcc
    10381 gacgacatgg cgaagctgct cgcgcggatc ggtgccaaga gcgacggtca ctaatcacgt
    10441 actttcgtac atacgcaacg cccgtcatga tatgagatga cttcgggtgt acggagtgct
    10501 gtcagacgag gagatcattc atggcctcgc cgatgacctt ccggtgggcc tcttcgtggc
    10561 tcgcgcacct ggcggcgagc tcgtttacgt caaccgcatg tttgcggaga tcatggggca
    10621 gagcggacgc gaggatgcct ccgctgggag ctactcggag ccgtacggca tcctgactcg
    10681 ggacggcaag ccgtacccgg aagagaagct gccgttcgtg ctcgcgctcg aggagaagcg
    10741 cgtcgttgtc gtcgacgaca tcatggttcg gcggcccgac cgcagcgtcg tgcacgtgcg
    10801 ggcgttcgcg aatccggtga tgaaggacgg cgaggtcacg cacgtcgtga tttgcttctt
    10861 cgacatcagc gaagaaatcg tcgcgaagct agcgcacgaa gagagtgagc ggcgcctcta
    10921 tcgctcgcag cgcctcgagg ccattggcac gctcgccggc ggcatcgccc acgacttcaa
    10981 caacctgatc ttcggcatca agctgctcgc ggccgagctg tcggccaacg agcacgatcc
    11041 gcaacggcgc gcgtcgctgc gcatgatcga cgacatcacc gagcgctcgg cgatgctcac
    11101 caagtcgctg cttggctttg ccggccgcgg caaccatcgt gcggtgccgg tcgcgctcaa
    11161 cgacgtgatc gtcggcatgc gcgagctgct cgcgcgcacg ctcggcggca tccaggtcat
    11221 cttccagctc gacgccacgc agcgtggcac ggtcatcggc gatcgcgcgc agctcgagca
    11281 ggtcgtgatg aacctggtcg tcaacgcacg cgacgcgctc aaggaaaagg gcggccgcgt
    11341 cgtcgtcaag acgaagagcg gggccgggac tgtcatgctc gaggttctcg acgacggccc
    11401 gggcattccc gtcgagctgc gcgagcgcat cttcgagcca tacttcacga cgaagacgag
    11461 cgggccgcag cgcggcaccg ggctcggcct cgcgaccgtg ttcgggattg tcgagcggca
    11521 caaaggcgcg atcgaagcgg ggccgggatt gcacggcgcg ggcgcggcga tgcgcgtgac
    11581 gttgccggcg gcacccgagg ccgcggacga agagccggcc gtcgatgacc ttggcctcga
    11641 gcccggaagc ggcacgattc tcgtcgtcga cgacgatctg atggtgcgcc gagcggtgtc
    11701 gatgacgctg cgcggccttg gctacacgac gttcgaggcg gcgagcggcc tcgaagcggt
    11761 cgatctgtac cgccagcatc acgcgatcat ccgcgcggtc gtgctcgacg tgatcatgcc
    11821 gggcatgagc gggcgtgcca cgtacgcggc aatgcgcgcg gtcgatccga cggtcgcggt
    11881 gctgctgatg tcgggctaca cgaagaacga ggacgtgcag gtgctgctcg agcagggcgc
    11941 gcgcggcttc gtgatgaagc cgtactcggt cgaagtgctc gcgcgctcgc tcgccgacgc
    12001 gctcaaacaa ccgaacgcgt agctactggt tacgcgcgcc gacgatccag cacgatgacg
    12061 gtgcatagca gccgtcaccg cgcatcatcg gctcgatcgc agatttcaac gcggtcacga
    12121 tctcgtcgcg acggcgaacg ccttcggcgc cagcgagccg cacgacctca cctgcaggcc
    12181 ccagcgtgag cgcgaagtcg atcgcttgct cgacggtact gccgaagaag aggtccgcat
    12241 cgctgcgttc gaacgtgatg tcggtccagc ccgcgccctt catctgatcg ctgacgagat
    12301 cagggctcgc catcgagaac ggacccggac cacacgtgac ctgatcgttc ttcggcgggt
    12361 caccgaggat gtcgcgcacg atgagctcgg cgttgtagaa cgcctcgttc gtctccttct
    12421 tgcgccacac gaccatgcgc agcggcgcgc ctggcgcgag cgccttgcga atgttgcgca
    12481 gcgcgatcac cggctggttg aagaacatcg tgcccatgcg cgagaacgcc gcgtcgtagg
    12541 gaccgccggg cacgccggcc tcgatgtcgg cgacctcgaa gtggacctgc tcgagccccg
    12601 ttgcctcggt gcgcgcggtc tcgatgaagc gcgacgcggc gtcgagcccg acggcgcggc
    12661 cgctcggccc gacctgctgg ccgagcacct gcgtcgtgtc gccgaagcca cagccgatgt
    12721 cgaccacctt catcccggcc ttgaggccaa gccgctcgat ggcgcggtta ccgtgctgcg
    12781 cgagcgcggt cgtcaccatc ggtcgatagg ccacgaactt gtcgaagagg accgtgttcc
    12841 acgcctctat cgcctcctga ttccccggca gcgtttgcat tgccgcaggc tagcccgacc
    12901 ggacgtgcta cgaaagaccc atggtgcctg acgcgtggcg gcacatcctc gagcgcgagg
    12961 ttgcggtcta tcgcaagctc gacgtccgcc agcgcgccaa gctcgtcgaa gatctactgg
    13021 ttttcgcggg caaggagttc gttggcctcg acggcttcgt cgtcgacgac cgcgtgcgcg
    13081 tccatgtgtc ggcgaccgcg gcgctgatgg tgctcgggat cggcatcgat cggttcgccc
    13141 atgtgcgccg catcgagatg cgcgaggcgc gctatctcga cgagcgcggc aacgtcgccg
    13201 aaggtcacta cgagtatgcg acgcacgaaa gcatcgggcc gatcggaatc ctccacctcg
    13261 cgtggaaggg catgctcgat ggcgccgctc gcaacgacgg cgcacacgtc ggcattcacg
    13321 ag
//
LOCUS       DASUFX010000092         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1543453, whole genome shotgun sequence.
ACCESSION   DASUFX010000092 DASUFX010000000
VERSION     DASUFX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543453"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1016
                     /locus_tag="VFV99_03600"
     CDS             123..1016
                     /locus_tag="VFV99_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5058415.1"
                     /translation="MSKHASPTVIGAFVVTALSLLLAGLLVLGGGKYFRSTAKLAVYF
                     DGSIAGLRVGAPVKFRGIDIGEVTSIKINMTGAIRDPQNVRVRVVIQIDEGLLSSQGV
                     NGIDLNDKKQVSMLVDRGLRAQLATESFVTGMRYVSLDIKPNAEAVLMNDRKYPEIPS
                     IRTSQQAITDKLERVLDKIADTDLPALIDSLKATIDDAHDLLRAPGLVRAIDRLDDVV
                     ASVEKTVADIAQTTRGFKPAVEQLTQAATSARKTVVSAEPLSHQLDVTLRDIQMAARS
                     VRRLSDQLSRDPGSILRGGKQ"
     gene            1013..1609
                     /locus_tag="VFV99_03605"
     CDS             1013..1609
                     /locus_tag="VFV99_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX5058416.1"
                     /translation="MMRAALLLMCACSFGGHRPNYQYFVLTTTAQRTPSSTASDESQP
                     TLAVDRVSLPGYLDREQIAMRTRDHSVIYSRTDRWAEPLEPAFERTLREDLAVHLRPA
                     GIQVPTRTEGPTYYLDVEVLRFERNGDRVELWARWTMRSSTEVLDTGDTHFFVPMRGV
                     DSNAMAVAASDAIAQMAAELAVQVRKTDRTLAARERRP"
     gene            1667..2188
                     /locus_tag="VFV99_03610"
     CDS             1667..2188
                     /locus_tag="VFV99_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058417.1"
                     /translation="MQAQRVIFLDTRHEQLPLAMREAYLADVQAWVLGTCRAAVRAGG
                     CSDRSALGLIEALYGHRSFEGSLARWRSIGPHASIRREFQLSDDAVSLMLLAAAPRLW
                     GALTHVYAAICMPGRNFVVSPLLLSMLLGSRPATLRELSADAPLVKYGLIAIRPSGAI
                     LASSEVVCRLAGS"
     gene            2208..3119
                     /locus_tag="VFV99_03615"
     CDS             2208..3119
                     /locus_tag="VFV99_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5058418.1"
                     /translation="MRIVSLLPSATEIVCALGAEAELVGISHECDFPPAIRDRRVLTR
                     SRIDAAGTSAAIDSAVRDVITNALSIYAIDDAALGALAPDVIVTQDLCEVCAVSLDDV
                     RAAVARLAHREDVQIVSLSPTRLADVLEDVTRVAAALGRKDRGAETRRALQQRIDAIA
                     ARVAKLPTRPRVVSLEWIEPLMVGGTWMPELIDLAGGTTIGATAGAPAPTITPPALAA
                     LAPEVVVIKPCGFAIPRTLAERAVIERDVLPHVGGARVYVTDGNAFFNRPGPRLVESL
                     EIMAACVHPSAFADFAAAHADTIVRLR"
     gene            complement(3121..3543)
                     /locus_tag="VFV99_03620"
     CDS             complement(3121..3543)
                     /locus_tag="VFV99_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX5058419.1"
                     /translation="MVVAVVMGVFAVMQIIPYGRDHSNPVANQEPAWDSPATRDLAVR
                     ACFDCHSNRTTWPWYSHVAPFSWVVQHNVDSARSVLNFSDWGHTYQMREQAAANVIAG
                     EMPPRSYRLMHPMAQLTDDEKIQLARGLQMTFGLPWRE"
     gene            3718..4029
                     /locus_tag="VFV99_03625"
     CDS             3718..4029
                     /locus_tag="VFV99_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058420.1"
                     /translation="MSFDDVARRMKSRHAEGMIPPMDTSPDVVRPAMDADAFAHQLIE
                     SERRAAKTRDLTIGGILLGLGLAITLITYDSASQSGGTYVVAYGPMIVGAIRLFKGLA
                     A"
BASE COUNT          694 a         1438 c         1269 g          696 t
ORIGIN      
        1 atctatctcg acgcgcagga gaagaccgtc accgcgctcg gcgatccgaa gcaaatgctc
       61 gcgcggccgc cgaacccgcg catcggcgca ttcctcacgc gcagcgccga caaggagtcc
      121 gcatgagcaa gcacgcctcg ccgaccgtca tcggagcgtt cgtggtgacc gcgctctcgc
      181 ttctcctcgc cggtctgttg gtcctcggcg gcggcaagta cttccgctcc actgcgaagc
      241 tagcggtcta cttcgacggc tcgatcgcgg gtctacgcgt cggcgcaccg gtgaagttcc
      301 gcggcatcga cattggtgaa gtcaccagca tcaagatcaa catgaccggc gcgatccgcg
      361 acccacagaa cgtgcgcgtg cgcgtcgtga tccagatcga cgagggtcta ctgagctccc
      421 aaggcgtgaa cgggatcgat ctcaacgaca agaaacaggt cagcatgctc gtcgatcgcg
      481 gcctgcgtgc gcagctcgct accgagagct tcgtcacggg aatgcgctac gtgtcgctcg
      541 atatcaagcc gaacgcggag gcagtcctga tgaacgatcg caagtatccc gagattccat
      601 cgatccggac ctctcaacaa gcgatcaccg acaagctcga gcgcgtgctc gacaagatcg
      661 ccgacaccga tcttcccgcg ctcatcgact cgctgaaggc caccatcgac gacgcccacg
      721 acctgctgcg cgcgccgggt ctcgtccgtg caatcgatcg cctcgacgac gtggtcgcgt
      781 cggtcgagaa gaccgtcgcc gacatcgcgc aaaccacgcg cggcttcaaa cctgctgtcg
      841 aacagctcac gcaagcagca acctcagcgc gcaagacggt cgtctccgcc gagccgctct
      901 cccatcagct cgacgtcacg ctgcgcgaca tccagatggc cgcgcgatcg gtgcgccggc
      961 tcagcgatca gctcagtcgc gatcccggat cgatcctgcg cggaggcaaa caatgatgcg
     1021 cgctgctctg ctcctcatgt gtgcgtgctc gtttggcggc catcgcccga actaccagta
     1081 cttcgtgctc acgacgacgg cgcagcgcac gccatcatcg accgcgagcg acgagtcgca
     1141 gcccacgctc gctgtcgacc gcgtctccct gcccggctat ctcgatcgcg agcagatcgc
     1201 gatgcgcacg cgcgaccata gcgtcatcta ctcgcgcacc gatcgctggg cagagccgct
     1261 cgagcccgcg ttcgagcgaa cgctgcgcga agacctcgct gtccatctga gacctgccgg
     1321 cattcaggta ccgacccgca ccgaaggccc gacgtactat ctcgatgtcg aggtgctgcg
     1381 cttcgagcga aacggcgacc gcgtggagct gtgggcgcgc tggaccatgc gatcgagcac
     1441 cgaagtgctc gacaccggcg acacgcattt cttcgtgccg atgcgaggcg tcgacagcaa
     1501 cgccatggcc gtcgccgcta gcgatgccat cgcccagatg gccgccgagc tggccgtgca
     1561 agtgcgcaag accgaccgca ccctcgccgc acgcgaacgg aggccgtagt tccaggcagt
     1621 taggcgcggc ttgcagggtt tgtctccgaa ccgcgtgtgt ctggatatgc aggcccaacg
     1681 cgtcatcttc ctcgacaccc gccacgagca actgccgctc gcgatgcgcg aggcgtatct
     1741 cgccgacgtg caggcgtggg tgctgggaac gtgtcgcgct gcagtccgcg ccgggggctg
     1801 cagcgatcgt tccgctctcg gattgatcga agcgctctac ggtcaccgca gcttcgaggg
     1861 cagcctcgcg cgttggcgct cgatcggtcc gcacgcgagt attcgccgcg agttccagct
     1921 gtcggatgac gcggtctcgc tgatgctgct cgccgcagcg ccgcgtctgt ggggcgcgct
     1981 cacgcacgtg tacgccgcga tctgcatgcc gggccgcaac ttcgtggtca gcccgctgtt
     2041 gctctcgatg ctgctcggca gccgccccgc cacgttgcgc gagctgtccg ccgacgcgcc
     2101 gctcgtcaag tacggtctga tcgcaattcg cccatcgggt gcgatcctcg cgagctccga
     2161 ggtcgtatgc cgcctcgccg gcagctgatc gggcatgatg cgcccgcatg cgtatcgtct
     2221 ctcttctgcc ctccgcgacc gagatcgtgt gtgcactcgg tgctgaagcc gagctcgtcg
     2281 gcatctcgca cgagtgtgac tttccgcccg ccattcgcga tcgccgggtg ctgacgcgct
     2341 cgcgcatcga cgcagccggc accagcgccg cgatcgattc cgccgtgcgc gacgtgatca
     2401 cgaacgcgct cagcatctat gcgatcgacg acgcggcact tggcgcgctc gcacccgacg
     2461 tgattgttac ccaggacctc tgcgaggtct gcgcggtatc gctcgacgac gtgcgcgccg
     2521 cagtcgcccg gctcgcgcat cgcgaggacg tccagatcgt gagcctcagc ccgacgcgac
     2581 tcgccgacgt gctcgaggac gtcacgcggg tcgcagctgc acttggccgc aaggaccgtg
     2641 gcgccgagac gcgccgcgcg ttgcagcagc ggatcgatgc gatcgcggcg cgtgtcgcca
     2701 agctgccaac ccggccgcgg gtcgtcagcc tcgaatggat cgagccgctg atggtcggcg
     2761 gcacgtggat gcccgagctg atcgatctcg cgggcggaac gacgattggc gcgaccgccg
     2821 gcgcacccgc accgacgatc acgccaccgg ccctcgccgc gctcgcgccc gaggtcgtcg
     2881 tgatcaaacc gtgcggattc gcgatcccac gcacgctcgc cgaacgcgcc gtgatcgagc
     2941 gcgatgtcct gcctcacgtc ggtggcgcgc gggtctacgt caccgacggc aacgcgttct
     3001 tcaatcgccc aggtccgcgc ctcgtcgaat cgctcgagat catggccgcg tgcgtgcacc
     3061 cgtcggcgtt cgccgacttc gcagccgcgc acgccgacac gatcgttcgc ttgcgttagc
     3121 tcactcgcgc cacggcagac cgaacgtcat ctgcagaccg cgcgcgagct ggatcttctc
     3181 gtcgtcggtg agctgcgcca tcggatgcat cagccgatag ctgcgcggcg gcatctcgcc
     3241 cgcgatcacg ttcgccgccg cctgctcacg catttgatac gtgtgacccc agtcggagaa
     3301 attcaacacg ctgcgtgcgg agtcgacgtt gtgctggacg acccacgaga acggcgcgac
     3361 gtgcgagtac cacggccacg tcgtgcggtt gctgtggcag tcgaagcacg cacggaccgc
     3421 gaggtcgcga gtcgccggcg agtcccacgc cggttcctgg ttcgcgaccg ggttggagtg
     3481 atcgcgtcca taaggaatga tctgcattac ggcaaacacg cccatcacca ccgcgaccac
     3541 catgaggaat cgtttgatga agcgaaacaa catagataca gcgggtccct ggtagcacgg
     3601 cgccccgccg agccgtccgc ggcctcctgt cgcactcgtt acaattgccc ccgtttcggg
     3661 cgtttggacg attgatcttt tcgtgcgacc ggcgcctagg gtaaggctct tcaccgcatg
     3721 agcttcgacg acgtcgcccg ccgcatgaaa agccgccacg ctgagggaat gatcccgccg
     3781 atggacacgt cgcccgacgt cgttcgcccg gcaatggatg ccgacgcgtt cgcgcatcag
     3841 ctgatcgaaa gtgaacgtcg cgccgccaaa acgcgcgacc tcacgatcgg tggcatcctg
     3901 ctcggcctcg gcctcgcgat cacgctcatc acctacgaca gcgcatcgca gtcgggcggc
     3961 acgtacgtgg tcgcgtacgg accgatgatc gtcggcgcga tccggttgtt caaaggcctc
     4021 gcagcctgag ctgcgatcgc agatcgcgta gtcgctcgcg cgaatgggca cgtttcactg
     4081 acaatgtcgt actcccg
//
LOCUS       DASUFX010000093         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_292774,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000093 DASUFX010000000
VERSION     DASUFX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292774"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFV99_03630"
     CDS             <1..489
                     /locus_tag="VFV99_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5058421.1"
                     /translation="LPINCLSWYDAMAFCIWDGGFLPTEAEWNYAASGGSEQRVYPWS
                     NPPTMEVVDCQHVNYYNGTDYCVNPPTGGTLRVGTHSPLGDGRWGHADIAGNIWEWVL
                     DYDAAYATPCDNCAQLTPGTNRGMRGGYFKNSMGNMRAANRSSNQPVDRPQYLGVRCA
                     RY"
     gene            complement(517..1554)
                     /locus_tag="VFV99_03635"
     CDS             complement(517..1554)
                     /locus_tag="VFV99_03635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058422.1"
                     /translation="MQPGGQLIDGKYQLVSVAGEGGMATVYKAVTKGAAGFQRTVAIK
                     HIKPEYRALKNYVDMFIEEARVGSELAHPNIVQVFDFIAEDGSYYLVMEWVEGIDLGH
                     FIRMHRDNNIHVEWPLAVAIGIGTLRGLGAAHDRRGADGTPSPVIHRDVTPHNILLGV
                     NGVVKLTDFGLARARDRVASMTAPGTVKGKLSYLAPEVSFGKTNTPQSDLFGVGSVMW
                     ETLAGERLFDGKSDVEIFKKIRACKVPPIGERRPDIPGSLVAVLDVALAANPADRFES
                     ATAFAHALSQVMKQAVGIDPQTALGQAVRELKTRGTSGSVEVKWSVEADYDVSRADSD
                     ADPIELTKPKK"
     gene            complement(1595..>2577)
                     /locus_tag="VFV99_03640"
     CDS             complement(1595..>2577)
                     /locus_tag="VFV99_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5058423.1"
                     /translation="YGEHVVFPSLDLTVRRGEKVGIIGVNGAGKTTLLRMIAGELPYD
                     SGAIKIGSGIEVGYYAQHHAETLDMSATIEEVLQRAAPDAPPARVRSMAGAFMFSGDD
                     IDKQCKVLSGGERARVALARLCLAPGNLLLMDEPTNHLDLASSESLAQSLKTYDGTLV
                     FVSHNRSLIRQLATRIWNVEDGRVETYPGTLDEYMYSMAQRRQALAATDGAPAAKGPA
                     VAPAAKTSREDDKARKRREAEARQKRTAKLGPLEKQVAQLEERVGVLEAEQKVRSAEL
                     ADPTVYDDAARRNKLLADYQSAQEKLEELTARWEAAMAELEAAKADLEAT"
BASE COUNT          393 a          860 c          852 g          472 t
ORIGIN      
        1 ctgccgatca attgcctcag ctggtacgac gcgatggcgt tctgcatctg ggacggcggc
       61 ttcttgccga ccgaggcaga gtggaactac gccgcgtcgg gcggttcgga gcaacgtgtg
      121 tatccgtggt cgaatccgcc caccatggag gttgtcgact gtcagcacgt caactactac
      181 aacggcaccg actactgcgt gaacccgccg accggcggca cgttgcgtgt cggtacccac
      241 tcgccactcg gcgacggacg ctggggccac gccgatatcg cgggcaacat ctgggagtgg
      301 gtcctcgact acgacgccgc gtacgcgacg ccatgcgaca actgcgcgca gctgacgccc
      361 gggacgaatc gcgggatgcg cggcggatac ttcaagaact cgatgggaaa catgcgcgcc
      421 gcgaatcgct cgagcaacca gcccgtcgac cggccgcagt acctcggtgt gcgctgcgcg
      481 cgctactgag ggccttcagt catccccgac gagggactac ttcttcggtt tcgtcagctc
      541 gatcggatcg gcgtccgagt ccgcgcgcga gacatcgtag tcggcctcga ccgaccactt
      601 cacctcgaca ctaccgctcg tgccgcgcgt cttgagctcg cgcactgcct ggccaagcgc
      661 ggtctgcgga tcgatgccga cggcttgctt catcacttgc gacagtgcgt gcgcgaacgc
      721 ggtcgcggac tcgaagcgat cggccggatt cgcggcgagt gcgacgtcga gcacggcgac
      781 gagtgagccg ggaatatcgg gccggcgttc gccgatcggc gggaccttgc acgcgcggat
      841 cttcttgaag atctcgacgt cgctcttgcc gtcgaacaac cgctcgcccg ccagcgtctc
      901 ccacatcacg ctgccgacgc cgaacaagtc gctctgcggc gtgttcgtct tgccgaacga
      961 gacctcgggc gcgaggtagc tgagcttgcc cttgacggtg cccggcgcgg tcatgctcgc
     1021 gacgcgatcg cgggcgcgcg cgaggccgaa gtcggtgagc ttcacgacgc cgttcacgcc
     1081 gagcaggatg ttgtgcggcg tgacatcgcg atggatgacc ggcgacggcg tgccgtcggc
     1141 accgcggcgg tcgtgcgcag cgccgagccc gcgcaacgtg ccgatgccga tcgcgacggc
     1201 gagcggccac tcgacgtgaa tgttgttgtc gcggtgcatg cgaatgaagt ggccgaggtc
     1261 gatgccttcg acccactcca tgacgaggta gtacgaaccg tcctccgcga tgaagtcgaa
     1321 cacctgcacg atgttcgggt gcgcgagctc cgaaccgacg cgcgcctcct cgatgaacat
     1381 gtcgacgtag ttcttgagcg cgcggtattc gggcttgatg tgcttgatcg cgacggtgcg
     1441 ctgaaaacca gcggcaccct tggtgaccgc cttgtacacg gtcgccatac cgccctcgcc
     1501 cgcgacggac acgagctggt acttcccatc gatcagctgc ccaccgggct gcacggaaca
     1561 agtatcgccc cagtcgccct agcctggaag gtacctacgt cgcttctagg tcggctttgg
     1621 cggcctcgag ctcggccatt gcagcctccc agcgggccgt cagctcttcg agcttttcct
     1681 gagcgctctg gtagtcggcg agaagcttgt tgcggcgtgc ggcatcgtcg tagacggtcg
     1741 ggtctgcgag ctctgccgag cgcacctttt gctcggcctc gagcacgccg actcgctctt
     1801 cgagctgcgc gacctgcttt tcgagcggcc cgagcttcgc ggtgcgtttt tgacgcgcct
     1861 cggcttcgcg ccgtttgcgc gccttgtcgt cttcgcgtga cgtcttggca gcgggagcga
     1921 cggctgggcc ctttgcagcc ggtgcgccat ccgtcgccgc gagcgcctgt cgccgctggg
     1981 ccatggagta catgtactcg tcgagcgtgc cggggtaggt ctcgaccctg ccgtcttcca
     2041 cgttccagat ccgggtcgcg agctggcgga tgaggcttcg gttgtggctc acgaacacga
     2101 gcgtgccgtc gtaggtcttg agcgactgcg cgaggctttc gctcgacgcg aggtcgaggt
     2161 ggttcgtcgg ctcgtccatc agcagcaagt tcccgggcgc gagacacagc cgtgcgagtg
     2221 cgacgcgcgc acgttcacca ccggagagca ccttgcactg cttgtcgatg tcgtcgcccg
     2281 agaacatgaa cgcgccggcc atcgagcgca cgcgcgccgg cggcgcatcg ggggccgcgc
     2341 gctgcaacac ctcttcgatc gtcgccgaca tgtcgagcgt ctcggcgtgg tgctgcgcgt
     2401 agtagccgac ctcgatgccg ctgccgatct tgatcgcgcc gctgtcgtac ggaagctcgc
     2461 ccgcgatcat gcggagcagc gtcgtcttgc cggcgccgtt gacgccgatg atgccgacct
     2521 tctcgccgcg ccgcaccgtg agatcgagcg acgggaacac gacgtgctcg ccgtacg
//
LOCUS       DASUFX010000094        11841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1821856, whole genome shotgun sequence.
ACCESSION   DASUFX010000094 DASUFX010000000
VERSION     DASUFX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821856"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1043)
                     /locus_tag="VFV99_03645"
     CDS             complement(54..1043)
                     /locus_tag="VFV99_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058424.1"
                     /translation="MRAGIALAFLWSLSLGSAAYADKNDLVLDRLSTRITDGSGNLMS
                     VVGQNLEFRSLASELGVVLAPHLLTPADTLGFGGFQLTVDYSSTTIDSDASYWRVLQS
                     SPDPAGTGGAAHGDGMMRTVGLFARKGMWFPIPSFEVGAGAVHLVDSHTWAGQLYAKV
                     ALHEGYHDLLIPSLAVRGSVSRMMNQRELDLTVASLDIAMSKHIGVGSTWRLDPFAGW
                     NLLMIVPRSEVIDPTPNIDPLQPGNQDDLLLNFVFKDQDAIIRNRLFIGAKFQYYVFQ
                     ATVEAVIAFAGKSVDDRTGTSDPCMPNSMTTVCDAKDTAGSQRTLSLSAGFDF"
     gene            1116..1883
                     /locus_tag="VFV99_03650"
     CDS             1116..1883
                     /locus_tag="VFV99_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5058425.1"
                     /translation="MPEYATILVDDRGPVGRITLNRPDKRNPIGPATCGELVAAFATL
                     KANAAVRAVILTGAGSVFSAGGDLSAMTQAQDGPIASLVDLFTTMHELGKPIIAMVNG
                     PALAGGLGLMVACDLVIAADTAVFGTTEISVGLWPMMITAEITRSVGRKKTLEMMLTG
                     RKLDANEAVECGLVNRAVPAAELEAETMKLASELAERSPAAIRLGLHAFYRSQDMELE
                     PQLRYLQAELGRVLALEDAREGITAFLQKRKPQWKGK"
     gene            1880..2233
                     /locus_tag="VFV99_03655"
     CDS             1880..2233
                     /locus_tag="VFV99_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5058426.1"
                     /translation="MKILRIDHVQLAIPPGGEAKARAFYGELLGLTEIPKPAPMAVRG
                     GMWFDGGIHLGIEPDMRPSVKMHPALVVDDLDALVATLVAAGYEWKPSDELPGVRRGH
                     TKDPFGNRIELIAAT"
     gene            2314..3105
                     /locus_tag="VFV99_03660"
     CDS             2314..3105
                     /locus_tag="VFV99_03660"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5058427.1"
                     /translation="MSRVAVAVLASAFVLAAASGEAARKKTATTSKAKSAAAKKAALA
                     KKKKLAMKKKSRAKGRHRVAVAEKVSTSKEGMPHGFAWPPNELMKTASVACEKHLDEI
                     GLTWERATPEGKIADPIYVPAMEIGGIRYVAAYGKGPRRLDCQLVKTLDQIGPELVKL
                     GVREIRYGSIYRNTNVRVHGTTKPILSRHALGIAMDIKAFLDDKGRVANVELDYLKGD
                     PLLHSIEDTVNASKLFRIVLTPGNDPLSHYDHFHFEAAVDYTAFR"
     gene            3115..3621
                     /locus_tag="VFV99_03665"
     CDS             3115..3621
                     /locus_tag="VFV99_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5058428.1"
                     /translation="MADPVVEVATNFGTFVIQLDQAHAPKSVENFLKYVDSKHYDGTI
                     FHRIIPTFMVQGGGYDQQYEKKRTLAPIQNEADNGLKNKRGTVAMARTGDPHSATAQW
                     FVNVVDNAFLDHTSKDDRGWGYTVFGTVTDGMDVVDKIKAVKTGANGPFAKDAPDEPV
                     VIKHVKRR"
     gene            3835..4536
                     /locus_tag="VFV99_03670"
     CDS             3835..4536
                     /locus_tag="VFV99_03670"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5058429.1"
                     /translation="MRSAAVLIALAGCAQGFATAPKPDAAPSELGQTFGVPGEAMEYQ
                     VSLRGVTVGRVQVAVGKAGFVDGKHAIIVKSRGTSAGLITLITNLKWELTTTLDMTTG
                     LPIREIEESWLEEDGEPEHHRNEREWNDHGFNLHAAAGALRGWHSRIGQRASFQVTID
                     RADIDVELWDAAREYLASANQPAVRYDGIALEKYSFSVWISDDEVRVPLFLRTGTKWG
                     EVTVELVRYDPPTEP"
     gene            complement(4538..5659)
                     /locus_tag="VFV99_03675"
     CDS             complement(4538..5659)
                     /locus_tag="VFV99_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058430.1"
                     /translation="MSNRGQRFVFGLIATVVGAVLFEVASSYIVFRGYPRWLAAGVGA
                     FAFPVGPLTWHLLAERKRKKKQADAKTPAKASLTAGDRYKLRFLVVALLVLGPMIAMG
                     RFNVARAVWKKGLWWWPTSYEPTTSHGGPLSTIGTGTPRTIDVIGTPLARVPPDAELV
                     VLVQPPPDDKKNEGHAILAWGDGQIMMMAEGKLDKTDKPEDMIRELNKARGKVPWLPM
                     GEVSLISSSDSQVLAATDRWKPLVELPGMGPSTDILRELGRAPKDAFFVAGFVPRTTR
                     DVLSTKSGAVWLYQTGEKLVFEGRAEMKDEAAAKQLVSEATRALDKAAHDAPESCREQ
                     VGALIKTIKLDQTGAAVTGRVELDGGVLMGVMFCAMKDK"
     gene            5739..6365
                     /locus_tag="VFV99_03680"
     CDS             5739..6365
                     /locus_tag="VFV99_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058431.1"
                     /translation="MRLLALAILLTAGTAAAHPPPEPEETIQWRTDRAFFEWSTWARL
                     GFGVERVPVDNVARTVTPPSTSDTHDQRTLWDAALGADVTLPLPTGRVRMGPWIELRP
                     KGVFAGGELSIAGDDLDMFWNDGEKVYTVRAGSSMTDLTAAIAYGYRCPWKLWGPYNH
                     ATRYMIGVRIVASATRNVQDPNDWSMSLGLEFEPVGSLRYVAGIKSWY"
     gene            complement(6335..7690)
                     /locus_tag="VFV99_03685"
     CDS             complement(6335..7690)
                     /locus_tag="VFV99_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5058432.1"
                     /translation="MSAVVMSFILATVVGEYMNSAIASRVRDMIGNAMPSVQLLGSAR
                     GDLGRLDLDLDEYAGAPDDRRSVLRDRLIGSRNDVEAALASYVSLPLFPNEWPLFAHV
                     QEGTAAVDTTLANYLAGAPTDRTGLQHVHQQVELLDQAIDRVVSFDASQGQRLGLEIE
                     GIRSETRAVVVLLAGLSVMLAIVAVFLSLRQLRRIARLERAERNALELRDRELSAENE
                     ALGQFAGSVAHDILSPLASAMLALDMVHESGDVDAESRRSAERGIMAVTRVKRLVDAL
                     LAFSRAGGRPEPGVSTEVAPVIADIVEGLGQQAREANITLIASSVPAGAIACSEGVLT
                     SVVSNLVTNAIKHMGNATQRRVDVRAFDLGTRWRFEVEDTGPGISPEQQAKIFQPYVQ
                     LDRGASGIGLGLATVDRLVRAHGGTVGVQSRGPGSLFWFELPKPAIATPVQYQLLIPA
                     T"
     gene            complement(7945..8460)
                     /locus_tag="VFV99_03690"
     CDS             complement(7945..8460)
                     /locus_tag="VFV99_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058433.1"
                     /translation="MGALSQARFEELVNAGCTCGGRLLEIRSYIDRSLVVMAAEPNNE
                     GRWAHDGEKFADGTYRITCATCAKVVFESDICPRCNAAGGLARALGETSRLTLPKRCP
                     QCNELELMALAFVPASARYGGEAPKPKQLVDFGDPGYHVVAYGCNSCDRAVVAEKCPL
                     CDAHGPLRPRP"
     gene            complement(8460..9350)
                     /locus_tag="VFV99_03695"
     CDS             complement(8460..9350)
                     /locus_tag="VFV99_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058434.1"
                     /translation="MAKKAKTEEAAPETETAIEAKPEQQIGFPKKTLYWIGGITAVIW
                     AFAISTGSMVFMIIIGVLTALLAFVLLRAFLFIKKQKNVVGLLQGAATSPEKRKEALE
                     KLEQAKDPNDPTNIFARSQLMAAEDPQGAIKLIEKTPLAKFPPAMQDDVALLKTQLYL
                     SLGRTADARKNADTINLDNPERKAARPLAGSIVAEAWARTGKPKEALALIDSIELPKK
                     DGEQIAIQLQVARVFAKFAANQRKQAETELVALSDQDVNLLGRFLDPRFRVHPELQKL
                     ARRVLERNPAARRKIQVQRR"
     gene            9464..10948
                     /locus_tag="VFV99_03700"
     CDS             9464..10948
                     /locus_tag="VFV99_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5058435.1"
                     /translation="MASRAGTVALVILKVVGRALWVSTMVLIPLFGFWLASSLAAYHN
                     ATQWLSLLVGLLLFPILPVGWDLLYAWRQKRKKYDGPRYLTGVDRLVLRTLLINAVFL
                     GGMMWKAKQESFRALAVRGDWMLDGFNGPVANTMRGWLLGFADHFDKRTTTVVENQYG
                     KGDQAPKDIKPIEDTETPGEKPTNPMGWPLAVAFDPIVAEIPDSEQTSIESVGAYFAA
                     RISDKKQLVKALHDYVVNRLHYDYDALKLIEAHDYEHTPAQTAEAVFARRAGVCEGYA
                     RLMVALGNAAGVEIAFVTGYIRDSARRLAVSDDPWDTSSREALEGVGHAWNAVKLDGQ
                     WYLMDVTWDDPTKGENSTTYLFVPPKLMAFDHYPEDSNWQLLEKPMSLGDFVRQPLLS
                     PRIGELGITLLSPTRSQVTVDGTVEIVLDNPYGAEVMADAHRDGSSHDDGGSPCKVTS
                     GTHTTITCNLADGEYEVQMFAAPAAQRTGRYTLDYVGSILVNSH"
     gene            11038..>11841
                     /locus_tag="VFV99_03705"
     CDS             11038..>11841
                     /locus_tag="VFV99_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX5058436.1"
                     /translation="MKRFLALALLAACGDSPTDIPEIKSDVARDLQPNVSAGDFATLV
                     DGNTEFATDLYAQVHGQSGNLFMSPHSISIALAMTWAGANTNTAAQMADTLHFTLPPD
                     QLHAAFNKLDLELASRAANAMSGTIPFKLKTANAIFGQEGKTFLDPFLDALARNYGAG
                     MRVLDFLSDPEGSRATINHWVEDQTNDRIKDLLPMGSVTPDTRLVLSNAIYFSAAWDE
                     PFKAEETADRAFKLGDGTTISVPTLHQVHERGYGEGDGYKAAELPYDGGQ"
BASE COUNT         2098 a         3891 c         3867 g         1985 t
ORIGIN      
        1 acggcttcta ctgcgaggcg cacagcttcg caggcactgt cacggtgcag tagtcagaag
       61 tcgaagccgg cggatagcga cagcgtgcgc tggctgcccg ccgtgtcctt ggcgtcgcac
      121 accgtcgtca tcgaattcgg catgcacgga tcgctcgtac cggtgcgatc gtcgacggac
      181 ttgccggcaa acgcgatcac ggcttcgacg gtcgcctgaa acacgtagta ctggaacttc
      241 gcgccgatga agagccggtt gcggatgatc gcgtcctgat ccttgaacac gaagttgagg
      301 agcaggtcgt cctggttacc cggctgtagc ggatcgatgt tcggcgtcgg atcgatgact
      361 tccgagcgcg gcacgatcat gagcaggttc cagccagcga acgggtcgag ccgccacgtg
      421 ctgccgacgc cgatgtgttt cgacatcgcg atgtcgagcg atgcgacggt caggtcgagc
      481 tcgcgctggt tcatcatgcg cgacaccgag ccgcgcaccg cgagcgacgg gatcaacagg
      541 tcgtgatagc cctcgtgaag cgcgaccttc gcgtagagct ggccggccca cgtgtgcgag
      601 tcgacgaggt gcaccgcacc ggcgcccacc tcgaacgacg ggatcgggaa ccacatgccc
      661 ttgcgcgcga acaggcccac cgttcgcatc atgccgtcgc cgtgcgccgc gccgccggtg
      721 cctgccgggt cgggcgagct ctgcaggacc cgccagtacg atgcgtcgct gtcgatggtc
      781 gtgctcgagt agtcgactgt gagctggaac ccgccgaagc cgagcgtgtc ggcgggcgtc
      841 agcaggtgcg gcgcgagcac gacgccgagc tccgaagcca gcgagcggaa ctcgaggttc
      901 tggccgacga cagacatcag gttgccgctg ccgtcggtga tgcgcgtgct caggcgatcg
      961 agaacgaggt cgttcttgtc ggcgtacgca gcggaaccca gagagagaga ccagaggaac
     1021 gcaagcgcga tcccagctcg cattctgcga gcgtaacacc ccagcttgcc gaactgaagc
     1081 cggcggcgcg gcccggttgc gtgctatgca gcggcatgcc cgagtacgcg acgatcctcg
     1141 tcgatgatcg cggtccggtc ggccgcatca cgctcaaccg ccccgacaag cgcaacccga
     1201 tcgggccggc gacctgcggc gagctggtcg cagcatttgc gaccctcaaa gccaacgccg
     1261 ctgtgcgcgc cgtcatcctc accggagccg gttcggtgtt ctccgccggt ggcgatctgt
     1321 cggcgatgac ccaggcccag gacggcccga ttgcgagcct cgtcgacctg ttcacgacga
     1381 tgcacgagct cggcaagccg atcatcgcga tggtcaacgg tcctgcgctc gcgggcggcc
     1441 tcggcctgat ggtcgcgtgc gatctggtga tcgcggccga cactgcagtg ttcggcacga
     1501 ccgagatctc ggttggcctg tggccgatga tgatcaccgc cgagatcacg cgttcggtcg
     1561 gccgcaagaa gacgctcgag atgatgctga ccgggcgcaa gctcgacgcc aacgaagcgg
     1621 tcgaatgcgg gctcgtcaat cgcgcggtgc cggctgccga gctcgaggcc gagacgatga
     1681 agctcgcgag tgagctcgcc gagcgtagtc ccgcggcgat ccggctcggc ctgcacgcgt
     1741 tctatcgctc gcaggacatg gagctcgagc cgcagctgcg ctacctgcaa gccgagctcg
     1801 gccgcgtgct cgcgctcgag gatgcgcgcg aaggcatcac ggcgttcctg cagaagcgca
     1861 agccccagtg gaagggcaag tgaagatcct ccgcatcgat cacgtgcagc tcgccatccc
     1921 gcccggcggc gaagcgaagg cgcgcgcgtt ctacggcgag ttgctcggtc tcaccgagat
     1981 cccgaagcct gcgccgatgg ccgtacgcgg cggcatgtgg ttcgacggcg gcatccatct
     2041 cggcatcgag cctgacatgc ggccgtcggt gaagatgcac cctgcgctgg tcgtcgacga
     2101 tctcgatgcg ctggtcgcga cgctcgtcgc ggcaggctac gagtggaagc catccgacga
     2161 gctcccgggc gtgcggcgcg gccacacgaa ggacccgttc ggcaatcgca tcgaattgat
     2221 cgctgccacg taattacgtg gagctcaaag tatcagcgct tactacagaa attgtcagcg
     2281 gtaagcctac gcgggccctg ctacctttga gctatgtcgc gggtcgctgt tgcggtgctg
     2341 gcgagcgcat tcgtgctcgc ggccgcatcg ggcgaagcgg ctcgcaagaa gaccgcgacg
     2401 acgtcgaagg cgaagagcgc cgccgcgaag aaggccgcgc tcgcgaagaa gaagaagctc
     2461 gcgatgaaaa agaagtcgcg cgcgaagggc cggcatcgcg tcgcggtggc cgagaaggtc
     2521 agcacgagca aagaaggcat gccgcacggc ttcgcgtggc cgccgaacga gctgatgaag
     2581 accgcgagcg tcgcgtgcga gaagcatctc gacgagatcg ggctgacgtg ggagcgcgcg
     2641 acaccggagg gcaagatcgc cgatccgatc tacgtgccgg ccatggagat cggcggcatc
     2701 cggtatgtcg ccgcgtatgg caagggaccg cgccgcctcg actgccagct ggtaaagacg
     2761 ctcgatcaga tcgggccgga gctcgtgaag ctcggcgtgc gcgagattcg ttacggcagc
     2821 atctatcgca acacgaacgt gcgcgtgcac ggcacgacga agccgatcct gtcgcggcac
     2881 gcgctcggga tcgcgatgga cataaaagct ttcctcgacg acaagggcag ggtcgcgaat
     2941 gtcgagctcg actacctgaa gggcgatccg ctcttgcact ctatcgagga cacggtcaac
     3001 gcgagcaagc tgttccgcat cgtgctcact cccggcaacg acccgctgtc gcattacgac
     3061 catttccact tcgaagcggc ggtggactac acggcgtttc ggtagtcttc tggcatggcc
     3121 gatccagtcg tcgaagtcgc aaccaacttc ggaacctttg tcatccagct cgatcaggcg
     3181 cacgcgccga agtcggtcga gaacttcctc aagtacgtcg acagcaaaca ctacgacggc
     3241 acgatctttc accgcatcat cccgacgttc atggttcagg gcggcggcta cgatcagcag
     3301 tacgagaaga agcgcacgct ggcgccgatc cagaacgagg cggacaacgg gctcaagaac
     3361 aagcgtggca ccgtcgccat ggcgcggacg ggcgatccgc actcggcgac cgcacaatgg
     3421 ttcgtcaacg tcgtcgacaa tgcgttcctc gatcacacgt cgaaggacga ccgcggttgg
     3481 ggctacacgg tgttcggcac ggtcaccgac ggcatggacg tcgtcgacaa gatcaaagcg
     3541 gtgaagaccg gcgcgaacgg cccgtttgcg aaggacgcac cggacgagcc ggtcgtcatc
     3601 aagcacgtca agcgccgcta gagcgtcgac gctcacgcgg attcgcacgg ccgcctcgcg
     3661 cgaacacgcg cggccgcaat gcgcgaacac gcgcggccgc atcgcgcggg ggaaccaccc
     3721 ctgcgagccg gcgagcgtgt tcgcgttctg cagagcggtg cacggcgagt gcgaaagacg
     3781 cgcgacgacg attttgtgca ccaaacggag gctcgatgcg tcttctagga cgccatgcga
     3841 tccgccgccg tcctcatcgc gctcgccgga tgtgcccaag gctttgcaac cgcaccgaaa
     3901 cccgacgcag cgccgagcga gctcggtcag acgttcggtg tcccaggcga ggcgatggag
     3961 tatcaggtct cgctgcgcgg cgtgaccgtc gggcgcgtgc aggtcgctgt gggcaaggcc
     4021 ggcttcgtcg acggcaagca cgcgatcatc gtgaagtcgc gcggtacgag cgctggcctg
     4081 atcacgttga tcacgaatct caagtgggag ctgacgacga cgctcgacat gacgaccggc
     4141 ttgccgattc gcgagatcga agagagctgg ctcgaggaag acggcgaacc cgagcatcat
     4201 cgcaacgagc gcgagtggaa cgatcacggc ttcaacctgc acgcggccgc cggtgcgttg
     4261 cgtggttggc actcgcggat cggtcagcgc gcgagcttcc aggtcacgat cgatcgcgcg
     4321 gacatcgacg tcgagctctg ggacgcagct cgcgagtacc tcgcgtcggc gaaccagccc
     4381 gcggtgcgct acgacggcat cgcgctcgag aagtacagct tctcggtgtg gatctccgac
     4441 gacgaagtgc gcgtgccgct gtttctgcgc acgggaacga agtggggcga ggtcacggtc
     4501 gagctcgtgc gctacgaccc accgaccgag ccgtagctca cttgtccttc atcgcgcaga
     4561 acataacgcc catcagcacg ccgccgtcga gctcgacgcg gccggtcacc gcagcgccgg
     4621 tctgatcgag cttgatcgtc ttgatcagcg cgccgacttg ttcgcggcag ctctcgggtg
     4681 cgtcgtgcgc ggccttgtcg agtgcgcgtg tcgcttcgga aacgagctgc ttcgcggccg
     4741 cctcgtcttt catctcggcg cgaccttcga acacgagttt ctcgccggtc tgatagagcc
     4801 acaccgcgcc ggacttcgtc gacagcacgt cgcgtgtcgt cctcggcacg aagcccgcga
     4861 cgaagaatgc gtccttcggt gcgcggccga gctcgcgcag gatgtcggtg ctcgggccca
     4921 tgcccggcag ctcgacgagc ggcttccagc gatcggtcgc cgcgagcact tgcgagtcgg
     4981 acgagctgat cagcgagacc tcgcccatcg gtagccacgg cactttgccg cgcgccttgt
     5041 tgagctcgcg aatcatgtcc tcgggtttgt cggtcttatc gagcttgccc tcggccatca
     5101 tcatgatctg gccgtcgccc cacgcgagga tcgcgtggcc ttcgttcttc ttgtcatcgg
     5161 gtggcggctg cacgagcacg accagctccg catcgggtgg cacgcgcgcg agcggagtac
     5221 cgatcacgtc gatcgtgcgt ggcgtgccgg taccgatcgt cgatagcgga ccgccatgcg
     5281 acgtcgtcgg ttcgtagctc gtcggccacc accacaggcc cttcttccac acggcacgcg
     5341 cgacgttgaa gcgccccatc gcgatcatcg gaccgagcac gagcagcgcg acgacgagga
     5401 agcgcagctt gtagcgatcg cccgcagtga gcgacgcctt cgccggcgtc ttcgcgtcgg
     5461 cttgcttctt cttgcgcttc cgctcggcga gcagatgcca ggtcagcggt ccgaccggga
     5521 acgcaaacgc accgacgccg gcggcgagcc agcgcgggta gccgcgaaac acgatgtatg
     5581 acgaggcgac ctcgaacagc accgcaccca cgacggtggc gatgaggcca aacacgaagc
     5641 gttgtccgcg gttcgacacg caaaaagcgt agcacccgga tgcagcacgt tcccggagat
     5701 ttcttggacc agcttccggc gctgtgcgtc caagggacgt gcgcttactc gccctcgcga
     5761 tcctgctaac tgccggtacg gctgccgcgc atccgcctcc ggagcccgag gagacgatcc
     5821 agtggcggac cgaccgcgcg ttcttcgagt ggtcgacctg ggcgcggctc gggtttggcg
     5881 tcgagcgcgt gcccgtcgac aatgtggcgc gcaccgtgac tccgccgagc acgagtgaca
     5941 cccacgacca gcgcacgctg tgggatgccg cactcggcgc cgacgtcacg ctcccgttgc
     6001 cgaccggcag agtgcggatg ggcccgtgga tcgagctgcg accgaagggt gtgtttgccg
     6061 gcggtgagct gtcgattgcc ggcgacgacc tcgacatgtt ctggaatgac ggcgagaagg
     6121 tctacacggt gcgcgctggc tcgagcatga ccgacctgac cgcggcgatc gcctacggct
     6181 atcgctgccc gtggaagttg tggggtccgt acaaccacgc gacgcgctac atgatcggcg
     6241 tgcgcatcgt cgcgtcggcg acgcgcaacg tgcaagaccc gaacgactgg tcgatgtcgc
     6301 tcgggctgga gttcgaacca gtcggctcgc tccgctacgt cgccgggatc aagagctggt
     6361 actgaacagg ggtggcgatg gccggcttgg ggagctcgaa ccagaacagc gaaccgggtc
     6421 cacgcgactg cacaccgact gttcccccgt gcgcacgcac gagccggtcg accgtcgcca
     6481 gcccgagccc gatgccactg gcgccgcgat cgagctgcac gtacggttgg aagatctttg
     6541 cctgttgctc gggtgagatg cccgggcccg tgtcttcgac ttcgaaccgc catcgagtgc
     6601 ccagatcgaa ggcgcgcacg tcgacccggc gctgcgtcgc attgcccatg tgcttgatcg
     6661 cattcgttac gaggttcgag acgacgctgg tcagcacgcc ctcgctgcac gcgatcgcac
     6721 cagccggaac gctggatgcg atcagggtga tgtttgcctc gcgtgcttgt tggccaagcc
     6781 cctcgacgat atccgcgatc accggtgcga cctcggttga cacgccaggc tcgggtcttc
     6841 cacccgcacg cgagaacgcg aggagcgcgt cgacgagcct cttcacgcgc gtcacggcca
     6901 tgatgcctcg ctccgccgag cggcgagatt cagcatcgac gtcgcctgat tcgtgaacca
     6961 tgtcgagcgc gagcatcgca gatgccagcg ggctcaaaat gtcgtgcgcg acgctccccg
     7021 cgaactggcc gagcgcctcg ttctcggcag agagctcgcg gtcgcgcaac tcgagtgcgt
     7081 tgcgttcggc gcgctctaat cgggcgatcc tccgtagctg gcgcagcgag agaaataccg
     7141 cgacgatcgc gagcatgacg ctcagaccag cgagcagcac cacgacggcc ctggtctcag
     7201 agcggatgcc ctcgatctcg agccccagcc gctgtccctg cgaggcgtcg aacgacacga
     7261 cgcggtcgat tgcctggtcg agaagctcga cctgttgatg cacgtgctgg aggcctgttc
     7321 gatcggtcgg cgcgcccgcc aaatagttgg ccagagtcgt gtcgacggcc gcagttccct
     7381 cctggacgtg cgcgaacagt ggccactcgt tcgggaagag cggcagcgac acatacgacg
     7441 caagcgccgc ctcgacatcg ttgcgcgagc cgatgagtcg atcccggagt accgagcgtc
     7501 gatcatcagg tgcgccagcg tactcgtcga gatcgagatc gaggcgtccg agatcaccac
     7561 gcgcgctacc gagcagctgc acgctcggca tcgcgttgcc gatcatgtcg cgtactcggc
     7621 tcgcgatcgc actgttcatg tactcgccga cgaccgtcgc caagatgaag ctcatgacga
     7681 ccgcggacat gactacgacc aaccaccgtg ctgtggagac cgatcggcgg gtgctggcct
     7741 cgctggccgt gctcataacc acagctaccc gacggggatc gcatcgacaa gctgcctgga
     7801 tcgcgctgcg acaacctcgg tgtgcgcagc accaaagcca tcgcagagtg cgagcccgtg
     7861 taggatttcc tccgtcggcg ttcgcgacta cgcgaaaccg tcgttcgtcg gcgcggatcg
     7921 agctccagcg ccgggacgat tttactaggg gcgcggccgc aacggaccgt gcgcatcgca
     7981 cagcgggcac ttctcggcga cgacggcgcg atcgcacgaa ttgcagccgt acgcgacgac
     8041 gtgataaccg gggtcgccga aatcgacgag ctgcttcggc ttcggcgctt cgccgccgta
     8101 acgcgcactc gccggcacga acgcgagtgc catcagctcg agctcgttgc actgcgggca
     8161 gcgcttcggc agggtcagcc ggctcgtctc gccgagtgcg cgtgccaggc cgcctgccgc
     8221 attgcaacgc ggacatatgt cgctctcgaa cacgaccttc gcgcacgtcg cacacgtgat
     8281 ccggtacgtg ccgtcggcga atttctcgcc gtcatgcgcc cagcggccct cgttgttcgg
     8341 ctcggccgcc atcacgacga ggcttcgatc gatgtagctg cggatctcca gcaaccgacc
     8401 gccgcacgtg cagccagcgt tgacgagctc ttcaaagcga gcttgcgaga gcgcgcccat
     8461 cagcggcgct gcacttggat cttgcgacgc gcggcaggat tccgctcgag tacgcgacgc
     8521 gcgagcttct gcagctccgg gtgcacgcgg aagcgcggat cgaggaagcg gccgaggagg
     8581 ttcacgtcct gatcgctgag cgcgacgagc tcggtctccg cctgcttgcg ctgattcgcc
     8641 gcgaacttcg cgaacacgcg cgcgacctgc aactggatcg cgatctgctc gccatccttc
     8701 ttcggcagct cgatcgaatc gatcagcgcg agcgcttcct tcggcttgcc ggtgcgcgcc
     8761 cacgcctcgg cgacgatcga gccggcgagc ggccgcgctg ctttgcgctc cgggttgtcg
     8821 aggttgatcg tgtccgcgtt cttgcgggcg tcggcggtgc ggccgagcga caagtagagc
     8881 tgcgtcttga gcagcgcgac gtcgtcctgc atcgccggcg ggaacttcgc gagcggcgtc
     8941 ttctcgatca gcttgatcgc gccctgcgga tcctcggcag ccatcagctg cgagcgcgcg
     9001 aagatgttcg tcggatcgtt cggatccttc gcctgctcga gcttctcgag cgcttccttg
     9061 cgcttctcgg gcgacgtcgc cgcgccctgc agcaggccca cgacgttctt ctgcttcttg
     9121 atgaacaaga acgcgcgcag caggacgaac gccagcagcg ccgtgaggac gccgatgatg
     9181 atcatgaaca ccatgctgcc ggtgctgatc gcgaacgccc agatcacggc cgtgatgccg
     9241 ccgatccagt agagcgtctt cttcgggaat ccgatctgct gctcgggctt cgcctcgatc
     9301 gcagtctcgg tttcgggtgc agcttcctcg gtcttggcct tcttcgccac ggcggggggc
     9361 agactagcgc acccgcccgc cggcacaatg gccgatcgtc gctacttagc ggcttctccg
     9421 aaactctttg cgcggacggc acatcgtagt taccacctaa gccatggcat cccgtgccgg
     9481 gacggtcgcg ctggtcattc tgaaggtcgt gggtcgggcg ctctgggtga gcacgatggt
     9541 gctgatcccg ctgttcgggt tctggctcgc gtcgtcgctc gctgcctacc acaacgcgac
     9601 ccagtggctg tcactgctcg tcggtctctt gctgtttccg atcctgccgg tcggctggga
     9661 tctgttgtac gcctggcgcc agaagcgcaa gaagtacgac gggccgcgct atctcaccgg
     9721 cgtcgatcgg ctcgtgctcc gcacgctgct catcaacgcg gtctttctcg gcggcatgat
     9781 gtggaaggcg aagcaggagt cgttccgcgc gctcgcggtg cgcggcgact ggatgctcga
     9841 cggcttcaac ggcccggtcg cgaacacgat gcgcggttgg ctgctcgggt tcgcggacca
     9901 cttcgacaag cgcacgacca ccgtcgtcga gaatcagtac ggcaaaggtg accaggcgcc
     9961 gaaggacatc aaaccgatcg aggacaccga gacgcctggt gagaagccga ccaacccgat
    10021 gggctggccg ctggcggtcg cgttcgatcc gatcgtcgcc gagattccgg acagcgaaca
    10081 gacgtcgatc gaatcggtcg gcgcgtactt cgcggcgcgc atctccgaca agaagcagct
    10141 cgtcaaggcg ctacacgact acgtcgtcaa ccgcctgcac tacgactacg acgcgctgaa
    10201 gctcatcgag gcgcatgatt acgagcacac gccggcgcaa accgccgagg ccgtgttcgc
    10261 gcggcgcgcc ggtgtatgcg agggctacgc gcggctgatg gttgcgctcg gcaatgccgc
    10321 cggcgtcgag atcgcattcg tcactggcta catccgcgac tcggcccgca ggctcgcggt
    10381 cagcgacgat ccgtgggaca cgagctcgcg cgaggcactc gagggtgttg gccacgcatg
    10441 gaacgcagtg aagctcgacg gccagtggta cctgatggac gtcacgtggg acgatccgac
    10501 gaagggtgaa aactcgacga cgtacctgtt cgtgccgccg aagctgatgg cgttcgatca
    10561 ctatcccgaa gactcgaact ggcagctgct cgagaagccg atgtcgctcg gcgacttcgt
    10621 gcgccagccg ctgttgtcac cgcgcatcgg cgagctcggc atcacgctgc tgtcgccgac
    10681 gcgctcgcag gtcaccgtcg acgggacggt cgagatcgtg ctcgacaatc cctacggcgc
    10741 ggaggtgatg gccgacgcgc accgtgatgg cagctcgcac gacgacggcg gcagcccgtg
    10801 caaggtcacg agcggcacgc acacgacgat cacgtgcaac ctcgccgatg gcgaatacga
    10861 ggtgcagatg tttgccgcac cggccgcgca acgtaccggg cgctacacgc tcgattacgt
    10921 cggctcgatc ctcgtgaata gtcactgagc gtctcggaaa actgagtcgg tgctgcattc
    10981 aagtgtgcga gatggcatgc gcacgtcgtg gcacatgcct tgaagtcgca ggcccgcatg
    11041 aaacgtttcc ttgcgctcgc tcttctcgcc gcgtgcggcg acagcccgac cgacattccc
    11101 gagatcaagt ccgacgtcgc gcgcgacctg cagccgaacg tcagcgccgg cgacttcgcg
    11161 acgctcgttg acggcaacac cgagttcgcg accgacctct acgcgcaggt gcacggccag
    11221 tccggcaacc tcttcatgtc gccgcactcg atctcgatcg cgctcgcgat gacgtgggcc
    11281 ggcgcgaaca cgaacaccgc ggcgcagatg gccgacacgc tgcactttac gctgccgccc
    11341 gatcagctcc acgccgcgtt caacaagctc gatctcgagc tcgcttctcg cgctgcaaat
    11401 gcgatgagcg gcacgattcc gttcaagctc aagaccgcga acgcgatctt cggccaggag
    11461 ggcaagacgt tcctcgatcc cttcctcgac gcgctcgccc gcaactacgg cgccggtatg
    11521 cgcgtgctcg atttcctgtc cgatcccgaa ggctcgcgcg cgacgatcaa ccactgggtc
    11581 gaagatcaaa cgaatgatcg catcaaggac ctgctgccga tgggctcggt gacgcccgac
    11641 acgcggctgg tactctcgaa cgcgatctac ttctccgccg cgtgggacga gccgttcaag
    11701 gccgaagaga ctgcggatcg cgcattcaag ctcggcgacg gtacgacgat ctcggtaccg
    11761 acgctgcacc aggtacacga gcgcggttac ggcgaaggcg atggctacaa ggcagccgag
    11821 ctgccgtacg acggtggcca g
//
LOCUS       DASUFX010000095         6803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_432231,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000095 DASUFX010000000
VERSION     DASUFX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432231"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VFV99_03710"
     CDS             complement(<1..1358)
                     /locus_tag="VFV99_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058437.1"
                     /translation="MEADGPDTIADGGPSLPVDDLQEEAAALIGVDVGGYVIDGELGR
                     GGMGVVFSATHPVIGKRAAIKVLKPSLSNNPATVERFVQEARSVNQIGHPNIVDIFAY
                     GALPDGRSYLVMDLLQGESLRKRVKRGPLLVGEALHVIDQVASALAAAHDKGFIHRDL
                     KPDNVFLVEHAGRTECKLLDFGLAKLLPTASMVRAYRTATGAQLGTPDYMSPEQLKGP
                     AGVDTRTDVFALGVLAFEILVGERPRRYSDGTFELGGKTISEAIMAKAKPPQELAQLV
                     EAMLALDKEKRPSLAAVHAVLKRARTAVPSTSVIGLEVSIPPVQANVATPRTGLPTVD
                     EGPRERAPSQPPGRGSQPLLRPSTPALSQPRPNQVRVSDAETNPGTFSQTPSSPALSA
                     NSAAGLRPAASTHGGTKLGVAPPGERPSKPHPVALRAMTGQPSGGGRTWLIVAVVLVV
                     VSA"
     gene            complement(1374..2681)
                     /locus_tag="VFV99_03715"
     CDS             complement(1374..2681)
                     /locus_tag="VFV99_03715"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5058438.1"
                     /translation="MNRGWFALWISALVSVIACGDNLPGEDRQGGDTTVDDRTRLAFR
                     QPAANATEADRARYQAGRSPFDFVWEVPKLGPLFNNSACLGCHGGNGRGMSQIGGMEP
                     TSQALVRVSLNEGEPGDPGGPIPVPGFGTQLQDHATVGLAEVRVTLTWIDSQVMYDDG
                     ELVDMREPRVDIRTPMGDLLPANTRFSYRIAPPLIGLGLLEAIPEADLRALADPDDAD
                     GDGISGKLNEVWDPVQQATVVGRFGWKANTSRLAIQVPGAFVNDMGLTSYVFPEVDGN
                     RDVNDEQLDDTVFFASIVGVPVAAPRDAAASRGRDLFDDFGCASCHVPTHTTGPHPIG
                     LVGRQRIHPYTDLLLHDVGDRLTDARPDFLAEGVEWRTPALWGIGLTQTVLPTATFMH
                     DGRARTLPEAILWHGGEAMSAREAFRTASRADRDALIAFLRTL"
     gene            complement(2711..3967)
                     /gene="lysA"
                     /locus_tag="VFV99_03720"
     CDS             complement(2711..3967)
                     /gene="lysA"
                     /locus_tag="VFV99_03720"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5058439.1"
                     /translation="MGPFQYRNNELYCEDVALAKIAEEVGTPVYVYSRSEIERAYRAF
                     DAALEGVPHEVCYAVKANSTLGVLGVLVQLGAGADIVSVGELYRWLRAGGDPDGVIFS
                     GVGKTDAEMKTALEAGIGCFNVESGEELIALDRVAQAIGKRAKISIRVNPDVDAQTHP
                     YISTGLKQNKFGVSVAEARQLFRDAAKAKGLQVAGVDFHIGSQLTKTSPFVDAIARLV
                     DLVQALASDGIKLEHVDIGGGLGIDYGKGETITSPAEYGKAVRAALQPLTSLGIKVLT
                     EPGRVIVGQAGALVTRVLFRKHNEAKHFTIVDAAMNDLMRPALYQAHHPMKPVKNPER
                     PVTATDIVGPICETGDFFARDRELPALEQGELLWIGAAGAYGSAMASNYNTRPRAPEV
                     LVSGATYSVIRTRETYEQLVANEKLV"
     gene            complement(3980..5362)
                     /gene="argH"
                     /locus_tag="VFV99_03725"
     CDS             complement(3980..5362)
                     /gene="argH"
                     /locus_tag="VFV99_03725"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5058440.1"
                     /translation="MSGPKGARGRRFEGALDPVAAAVNASVDFDKRLLPHDVAGSIAH
                     AEMLAAQGIISAADAKAIVDGLRRVEQRLANGELAWDEALEDVHMNVEARLIEEIGDA
                     GRRLHTARSRNDQVATDLRLYALSAAEKLIDAIDDVRRALIAQAKQHVDTLLPGYTHL
                     QRAQPVRLAHHLLAYEAMLDRDRGRIADAAHRADASPLGSGALAATTLPIDREHTARA
                     LGFSEVTTNSLDAVGDRDFAIELVAACALAQVHLSRLGEELVLWTSQEFGFAKIGEAY
                     CSGSSLMPQKMNPDLAELIRGKSGRVIGSWVALVTVVKGLPLAYNKDLQETQEPLYDA
                     VETVLACLQIARGMIASLTFDTQRMRAAIDAGHLVATEVADYLVTKGVAFREAHDIAG
                     HLVRVASERGVELAALDLETLRAAHAAFDTDVASWLDPARAVDRRDVVGGPARNRVLA
                     EIARIERELG"
     gene            5443..6066
                     /locus_tag="VFV99_03730"
     CDS             5443..6066
                     /locus_tag="VFV99_03730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5058441.1"
                     /translation="MLFLPDGGDKRGGVVLIHEWWGLNEQIESLCEKWTREGFVAFAP
                     DLYHGNVVPIGHAKEAEAAMMALDFPKALQEITGAVAYLKSHPRCNGKVAVTGFCMGG
                     ALSFATATTVPGLAAVVPYYGLPPSADWSRVDAPIQAHFAIEDDWATVDGAKAVQEQL
                     AKYKRPMELHAYAAKHAFCNDRRPEVYNAEACAQAWQRSVEFVRAYT"
     gene            6222..6611
                     /locus_tag="VFV99_03735"
     CDS             6222..6611
                     /locus_tag="VFV99_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058442.1"
                     /translation="MMTAMPQPMTNHNMGMPMMPMMNPMMGNMMSPMMGMNPMMGMNP
                     MMGMMNPMMGMMPMMMPMMGMGMPMMCNMTMEMGKDGMICKMMPMDMAQMDMMKERMS
                     QMMNMMKMGMPCMMMCGGMPMMMGSAS"
BASE COUNT         1167 a         2210 c         2273 g         1153 t
ORIGIN      
        1 gccgatacga caaccagaac gacggcgacg atcagccagg tccgaccgcc gccgctgggt
       61 tgtccggtca tggcccgtaa tgcgaccggg tgcggcttac taggacgttc gcctggaggt
      121 gcgacgccca gcttcgtgcc accgtgcgtg ctcgcagcag ggcgcaagcc cgcggcagaa
      181 ttggccgaca gcgccggcga gcttggcgtt tgcgaaaacg taccggggtt agtttcggcg
      241 tccgaaactc ggacctggtt cggacgcggc tgcgacagcg ctggcgtgct cgggcggagc
      301 aatggttgcg agccgcgacc cggcggctgc gacggtgcgc gctcgcgcgg cccctcgtcc
      361 accgtcggta acccggtccg cggcgtcgcg acgttcgcct gtaccggcgg gatgctgacc
      421 tcgaggccga tcaccgaggt cgacgggacc gccgtgcggg cgcgcttgag caccgcgtgc
      481 acggccgcga gcgacggtcg cttttccttg tcgagcgcga gcatcgcttc cacgagctgt
      541 gcgagctctt gcggcggctt cgccttcgcc atgatcgctt cgctgattgt cttgccaccg
      601 agctcgaacg tgccgtcgct gtagcgcctc ggccgttcgc cgacgaggat ctcgaatgcg
      661 agcacgccga gcgcgaagac gtccgtccgc gtgtcgacgc cggccggtcc cttcagctgc
      721 tcgggcgaca tgtaatcggg cgtgccgagc tgcgcgcccg tcgcagtccg gtatgcgcgc
      781 accatcgacg cggttggcaa cagtttcgcg agcccgaagt cgagcagctt gcactcggtg
      841 cgtcctgcgt gctcgacgag aaacacgttg tcgggtttca gatcgcggtg gatgaagccc
      901 ttgtcgtggg cagctgcgag cgccgacgcg acctggtcga tcacgtgcaa cgcctcaccg
      961 acgagcagcg gtccgcgctt gacccgtttg cgcagcgact cgccttgcaa cagatccatc
     1021 acgagataac tgcggccatc gggcaacgcg ccgtacgcga agatgtcgac gatgttcgga
     1081 tgaccgatct gattcaccga tcgcgcttcc tgcacgaagc gctcgacggt cgcaggattg
     1141 ttcgacaacg acggcttgag cactttgatc gccgcgcgct tgccgatcac cggatgcgtc
     1201 gcgctgaaca cgacgcccat tccaccgcgg ccgagctcgc catcgatcac gtagccgccg
     1261 acgtcgacac cgatcaacgc cgccgcttcc tcctgcagat cgtcgacggg aagtgaaggc
     1321 ccaccgtccg cgatcgtgtc cggtccgtcc gcctccacga accaaagtgt aggtcaaagc
     1381 gtccggagaa atgcgatcaa tgcgtcgcga tccgcgcgtg acgccgtgcg gaatgcttcg
     1441 cgtgcagaca tcgcctcacc gccgtgccac aagatcgcct cgggcagtgt gcgcgcgcgc
     1501 ccatcgtgca tgaacgttgc ggtcgggagt acggtctgcg tgaggccgat gccccacaac
     1561 gctggtgtgc gccactcgac accctcagcg aggaaatcgg gacgcgcatc ggtgagccga
     1621 tcgcccacgt cgtggagcaa cagatccgta tacggatgaa tgcgctgcct gccgacgagc
     1681 ccgatcggat gcggaccggt cgtgtgcgtc ggcacgtggc agctcgcgca accgaaatcg
     1741 tcgaacaagt cgcggccgcg tgacgcggca gcatcgcgcg gagcagccac cggtacgccg
     1801 acgatcgacg cgaagaacac ggtgtcgtcg agttgttcgt cgttgacgtc gcggttgccg
     1861 tcgacctccg ggaacacgta gctggtcagt cccatgtcgt ttacgaatgc gccgggcacc
     1921 tggatcgcca gccgcgacgt atttgctttc cagccgaacc ggccgacgac ggtcgcttgc
     1981 tggaccggat cccacacctc gttgagcttg ccggagatgc catcgccgtc cgcatcgtcg
     2041 ggatcggcaa gcgcgcgcag atccgcttcc ggaatcgctt cgagcaagcc gagaccgatc
     2101 agcggcggcg cgatgcgata cgaaaaccgc gtgttcgcgg gcagcagatc gcccatcggc
     2161 gtgcggatgt ccacgcgggg ctcgcgcatg tcgacgagct cgccgtcgtc gtacatcacc
     2221 tgcgaatcga tccacgtcag cgtcacccgt acctcggcga gaccgacggt cgcgtgatcc
     2281 tgcaattgcg tgccgaagcc aggcaccgga atcggaccgc ccggatcgcc gggctcgcct
     2341 tcattcaagc tgacgcgaac cagcgcctgc gacgttggct ccatgccgcc gatctgcgac
     2401 atgccgcgcc cgttgccgcc gtggcagccg aggcacgcac tgttgttgaa cagcggcccg
     2461 agctttggca cctcccacac gaaatcgaac ggactgcggc ccgcttggta gcgtgcgcga
     2521 tccgcttcgg tcgcattggc ggcgggctgc cgaaacgcaa gccgcgtgcg atcgtcgacg
     2581 gtagtgtcac caccctggcg atcttcgccg ggcagattgt caccgcacgc gatgacggag
     2641 acgagcgccg agatccaaag ggcaaaccac ccgcggttca cccgccaagg atacagcgac
     2701 cgcgagccga ctacaccagc ttctcgttcg cgacgagctg ctcgtacgtc tcgcgcgtgc
     2761 gaatcaccga ataagtcgca ccgctgacga ggacttcggg cgcgcgcggc cgcgtgttgt
     2821 agttcgaggc catcgcggag ccgtacgcac cggcggcgcc gatccagagc agctcgcctt
     2881 gctcgagtgc gggcagctcg cgatcgcgcg cgaagaagtc gccggtctcg cagatcgggc
     2941 cgacgatgtc ggtcgcggtg acgggacgct cgggattctt caccggcttc atcggatgat
     3001 gtgcctgata cagcgcgggc cgcatcaggt cgttcatcgc cgcgtcgacg atcgtgaagt
     3061 gcttcgcttc gttgtgcttg cggaacagca cgcgcgtcac gagcgcgccg gcctggccga
     3121 cgatcacgcg gccgggctcg gtgagcacct tgatgccgag cgacgtcagc ggttgcagcg
     3181 ccgcgcgcac cgccttgccg tactcggcgg ggctcgtgat cgtttcgccc ttgccgtagt
     3241 cgatgccgag gccaccgccg atgtcgacgt gctcgagctt gatgccgtcg ctggcaagcg
     3301 cttgcacgag atcgacgagg cgcgcgatcg cgtcgacgaa cggtgacgtc ttcgtcagct
     3361 gcgaaccgat gtggaagtct acgccggcaa cctgcaggcc cttcgccttc gcggcgtcgc
     3421 ggaacagctg gcgcgcctcg gcgaccgaga cgccgaactt gttctgcttg aggcccgtcg
     3481 agatgtacgg gtgcgtctgc gcgtcgacgt ccgggttgac gcggatcgag atcttggcgc
     3541 gcttgccaat cgcctgcgcg acgcgatcga gcgcgatcag ctcttcgccc gactcgacgt
     3601 tgaagcagcc gatgccggct tcgagcgcgg tcttcatctc tgcatcggtc ttgccgacgc
     3661 cgctgaagat gacgccgtcc ggatcgccac cggcacgcag ccagcgatac agctcgccga
     3721 ccgagacgat gtcggcgccg gcgccgagct ggaccagcac accgagcacg ccgagcgtcg
     3781 agttcgcttt gaccgcgtaa cagacttcgt gcggaacacc ttcgagtgca gcgtcgaacg
     3841 cgcgatacgc acgctcgatc tcgctccggc tgtagacgta gaccggcgtg ccgacctcct
     3901 cggcaatctt cgccagtgcg acgtcttcgc agtagagctc gttgttgcgg tactgaaacg
     3961 gacccatagg tccgctgttc tagcccaact cgcgctcgat gcgggcgatt tccgccagaa
     4021 cccgattccg tgccgggcca ccgacgacgt cgcgccggtc gacggcacgc gcaggatcga
     4081 gccatgaggc cacgtcggtg tcgaacgccg cgtgggcagc gcgcagcgtc tcgagatcga
     4141 gcgccgccag ctcgacgcca cgctcgctcg cgactcgcac gagatggcca gcgatgtcgt
     4201 gcgcttcgcg aaacgcgacg cccttggtga cgagataatc ggcgacctcg gtcgcgacca
     4261 gatggccggc gtcgatggcg gcgcgcatgc gctgcgtgtc gaacgtgagg ctcgcgatca
     4321 tgccgcgcgc gatctgcagg catgcgagca cggtctcgac ggcgtcgtag agtggctcct
     4381 gcgtctcttg cagatccttg ttgtacgcga gcggcaatcc cttcacgacg gtgacgagcg
     4441 caacccacga tccgatcacg cgcccgctct tgccgcggat cagctccgcg agatcggggt
     4501 tcatcttctg tggcatcagc gagctgcccg agcagtacgc ctcgccgatc ttcgcgaagc
     4561 caaactcctg cgacgtccac agcacgagct cttcgccgag ccgcgagaga tggacttgcg
     4621 cgagcgcgca cgccgcgacc agctcgatcg cgaagtcgcg atcgccgacg gcatcgagcg
     4681 agttggtggt gacctcggag aagccgagcg cacgcgcggt gtgctcgcga tcgatcggca
     4741 gcgtggtcgc ggcgagcgca cccgaaccga gcggcgaggc atctgcgcga tgagcggcgt
     4801 cggcgatgcg gccgcgatcg cgatcgagca tcgcttcgta tgcgagcagg tgatgcgcga
     4861 ggcgaaccgg ctgcgcgcgt tgcagatgcg tgtagccagg cagcagcgtg tcgacgtgct
     4921 gcttcgcttg tgcgatcagc gcgcggcgaa cgtcgtcgat cgcatcgatc agtttctcgg
     4981 cggcgctcag cgcatagagt cgcaggtcgg tcgcgacttg atcgttgcgg ctgcgcgcgg
     5041 tgtgaaggcg gcggccggcg tcaccgatct cttcgatcag ccgcgcctcg acgttcatgt
     5101 ggacgtcttc gagtgcttcg tcccacgcga gctcgccgtt tgcgagccgt tgttcgacgc
     5161 ggcgcagccc gtccacgatc gccttcgcgt cggccgcgga gatgatgcct tgcgcggcga
     5221 gcatctcggc gtgcgcgatc gagccggcga cgtcgtgcgg gagtaagcgc ttgtcgaagt
     5281 cgaccgacgc gttgacggcg gcggcgacag gatcgagcgc tccttcgaac cttcgtcctc
     5341 gtgcgccttt cggtccggac atcgcgcgca gtataaagcc gcccggaggg acccatggcg
     5401 caacgagtga cgtttccagc ggcaacaggc gaagcatccg gcatgctgtt tctgcccgac
     5461 ggtggcgaca aacgaggcgg agtcgtcctc attcacgagt ggtggggcct gaacgagcag
     5521 atcgagtcgc tgtgcgagaa gtggacgcgc gagggcttcg tcgcgttcgc gccggacctc
     5581 tatcacggca acgtcgtgcc gattggccac gcgaaggaag ccgaggcagc gatgatggcg
     5641 ctcgacttcc ccaaggcgct gcaagaaatc acaggcgcgg tcgcgtatct gaagagtcat
     5701 ccgcgctgca acggcaaggt ggcggtcacc ggcttctgca tgggtggcgc gctcagcttc
     5761 gcgaccgcga cgacggtgcc ggggctcgcg gccgtcgttc cgtactacgg gctgccgccg
     5821 agcgcggatt ggtcgcgcgt cgatgcgccg atccaggcgc acttcgcgat cgaggacgac
     5881 tgggcaaccg tcgacggtgc gaaggccgtg caagagcagc tcgcgaagta caagcggccg
     5941 atggagctgc acgcgtacgc ggcgaagcac gcgttctgca acgaccgtcg gcccgaggtc
     6001 tacaacgccg aggcgtgcgc gcaggcgtgg cagcggtccg tcgagttcgt acgcgcgtat
     6061 acgtagccat ccgcgatgtc ggcactcagt ccgcctcgac ggagatttct ccgaggagcg
     6121 cagacactcc tccgtgcaaa cagaggaatc atcctgccgt cgcaggtacg tcacggccca
     6181 atgcttgcgc cgtcaacggg tgaccatagg atgccagcat catgatgaca gccatgccgc
     6241 agccgatgac gaaccacaac atggggatgc cgatgatgcc gatgatgaac ccgatgatgg
     6301 gcaacatgat gagccccatg atgggtatga accccatgat gggcatgaac cccatgatgg
     6361 gcatgatgaa cccgatgatg ggcatgatgc cgatgatgat gccgatgatg ggcatgggca
     6421 tgccgatgat gtgcaacatg acgatggaga tggggaagga cggcatgatc tgcaagatga
     6481 tgccgatgga catggctcag atggacatga tgaaagagcg catgtcgcag atgatgaaca
     6541 tgatgaagat gggtatgccg tgcatgatga tgtgcggtgg catgccgatg atgatgggta
     6601 gcgcgtcgta agctgaccgc gatcacaagc gagagagccg ccacgtgcgg ctcttgtcgc
     6661 tttcggctca ggtcgccggc ggtgagatct ggccaccgcc cgtcgaggtc gcgcgataga
     6721 gcttgatgat gccggcgccg ccgccgccac caccgcccgc gccaggtgtc gtgcacgttc
     6781 cgctacacat ggatgcgccg ggt
//
LOCUS       DASUFX010000096         8269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_709725,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000096 DASUFX010000000
VERSION     DASUFX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709725"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="VFV99_03740"
     CDS             complement(<1..1865)
                     /locus_tag="VFV99_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058443.1"
                     /translation="MSSAAPQVAEPLPFGAPVAPASAKPAQPVPLWHARPGVPIAAVF
                     ATEDGSAVVSIDESNHARLWPTLDGKSEPWVLPLGMPVEAVVERDGDRFAIAARDESG
                     GVEIVAVTANGELTADVKLSPEPGFDAVIENGDGFLALRRDQTLVQFDRHGEEAGKLV
                     PPPGEHVMKLLHRGGRTLALVRTKEGMRGHWVAADALAWSDITPKLAINLEHVFLSPD
                     HQRLVTFSNSRGEHMLVDLETGKSSTFARDDKAPEGFPAGFMGSRLVFAFNDFELSEL
                     QWWTPNGNEVGVVGGSNYALEFVAMEHPVVTDNNVIAFSGHEIAIATMNTASSPSQVR
                     FLGYRTSRAKGVKSSPVGVVATIGSVATLLDDNVRVDKRVPALETIPFAKDLALIKFT
                     ATDWGEPVVNVSNGIDPDWLEDPNARPTLRGHSTPRIALFDLERKTELQRWPSAGALH
                     YEPATKLMAAERGNKITFSRFDPATRKFTDETMLTAPGSDVTLLDPALAGGNIALLTR
                     ARKGAVSVRAIRDLAAAQPAPTELTGNLEAIDRAGRIYLRVDADTIVVHGSEGEQRIT
                     GMTGWTLRPSPTGTQIAAVGKSRLMLLDARGQSVWSVGFPGVRDAAWTSEGALVVL"
     gene            complement(2003..3565)
                     /gene="proS"
                     /locus_tag="VFV99_03745"
     CDS             complement(2003..3565)
                     /gene="proS"
                     /locus_tag="VFV99_03745"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5058444.1"
                     /translation="MSKDAKPQTAITPTRADNYQDWYQEVIKAADLAENSDVRGCMVI
                     KPWGYALWENVQRVLDGMFKATGHVNAYFPLFIPVSFLEKEAAHVEGFAKECAVVTHH
                     RLEARNGKLIPVGELEEPLVVRPTSETIIGASFAKWVQSYRDLPLLINQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHASKDEAIEETMKMLGVYAEFAEQYMAMPVIQGEKTAGE
                     RFPGAVSTYCIEGMMQDRKALQAGTSHFLGQNFSKASGIQFTDESNKLEHAWTTSWGV
                     STRLVGGMIMTHADDDGMICPPKLAPAHVVIMPITRKPEDRQRVLDWCKALKKDLESQ
                     QYAGAPIRAQLDDRDVGGGGKAWDWIKKGVPIRLEVGPRDIDQDSVFMGRRDKGAKEK
                     SSMPRAEFIAKVGGILDEIQTALFERARTFRKEHTRVIDTKEEFYAFFADPPKKREND
                     PTPIHAGFALTHFDGDPALEAKIKDDLKVTVRCIPIVDRADSVLGITAEPGTCPFTGK
                     PSKQRVVWAKSY"
     gene            complement(3623..4894)
                     /locus_tag="VFV99_03750"
     CDS             complement(3623..4894)
                     /locus_tag="VFV99_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEX5058445.1"
                     /translation="MRALVLCLLCGCGRIGFEDVRDNARADAGDAGAPFVEAVGQLDA
                     TFGSAGVTIVPAGMYGSQPYAVVERADGGYFAVGVHEGASLSRYFGVFAFTQSGALDT
                     TYGTSGIADLGPTANDFGYGAIRGADGRTALVGDGNDGSGDLDNVTVALLDTDGRLVS
                     SFATNGFSRTDIGGLQLDDTSRGVAFLADRLVVCGTHDYVAMNSQIILLELGLDGTPL
                     AGFGTSGVVTDDFSTGIDECNAVIRMPSGDLVVSGRTAGRAMLAAYSPDGTRDAAFAS
                     GGFVRVGGASTNAYDVALAGDGDLVTCGDGNGVAMCARAGPDGTLRTSFGQSGLVMVP
                     SLSLFWAVEVQPNGKIVATGQTAANGSGVVVRLAADGTLDQSFGSGGMVTLPTSGDTE
                     LQGLLLQADGSIVTTGYVSGTLDRAVIARIR"
     gene            complement(4967..5155)
                     /locus_tag="VFV99_03755"
     CDS             complement(4967..5155)
                     /locus_tag="VFV99_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058446.1"
                     /translation="MLDAIPEPVFPEEDPYPAYRAPAPIETAQEPKSPIDKYVLAAVV
                     ATGTVLVKNKFVRRITEL"
     gene            complement(5190..6299)
                     /locus_tag="VFV99_03760"
     CDS             complement(5190..6299)
                     /locus_tag="VFV99_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEX5058447.1"
                     /translation="MTACADSGEEGDAADIQGEEKADGTVGIEVTARVKPGTTDAQLS
                     TKVPRRGYVFYAAEGTKVSLEVKQGGSSAGLDTVLKVYGPRLADGTYPKTLATDEDSG
                     YGKLSKIKELAITIPGFYLAEVSFGEAATPVDGKKARLELACNGTCDTTLPIDTDLGL
                     KWYRRSAERRALVVQAYSLAIEKLQAKVSAGISGPWGVVLDIDETTLNNSQYQQERLD
                     LGLGYSPGSWTDWVNRKAASPLDGVLDFTHSVKQLGGKVVFVSNRLAATECAQTEANL
                     MAAGIVYDEILCKTDTSDKNPRFMAVQNGTMPSTLPALKVVLFVGDNILDFPALTQDI
                     RKMPASAYAKFGQDFILVANSMYGSWEKNTDDNSQ"
     gene            complement(6518..7183)
                     /gene="tsf"
                     /locus_tag="VFV99_03765"
     CDS             complement(6518..7183)
                     /gene="tsf"
                     /locus_tag="VFV99_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5058448.1"
                     /translation="MEINATMIKDLRERTGAGMSDCKKALTEVSGDMDKAIDYLRAKG
                     LAKAAKKAGREATEGLVVSYIHGGGRIGVLVEINCETDFVARNEDFVAFTRDVAMQIA
                     AMAPQYVRKEEVPAEAVEHEKAVLIAKAKEDPKMAGKPDAILAKITEGQVTKWMKEIC
                     LLDQPFVKEPDKTIEQLQQELIAKIGENIKIRRFVRFELGEGLEKKKDDFAAEVAKQA
                     GLA"
     gene            complement(7189..8010)
                     /gene="rpsB"
                     /locus_tag="VFV99_03770"
     CDS             complement(7189..8010)
                     /gene="rpsB"
                     /locus_tag="VFV99_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5058449.1"
                     /translation="MRALLEAGVHFGHNTGRWNPKMKQFIFGARNGIHIIDLQHTVKL
                     FRQAFNAVVETTSSGDTILFVGTKKQAQEVIVEEATRSNQHYVTQRWLGGTLTNFRTV
                     KGSLERLRSLEKMEEDGTLFALTKREQVMIKRDRDKLLKSLGGIKGMSKLPGMLFVID
                     PHKEHIAVDEARKLEIPVVAITDTNCDPDQIDYVIPGNDDAIRAIKLFAARIADAAII
                     GQKVWKETARHRGQERQEEGERVIHVSSGGDGPRVEMSTGGSMFNPEASASPEGE"
BASE COUNT         1303 a         2760 c         2651 g         1555 t
ORIGIN      
        1 agcacgacga gcgctccctc ggatgtccac gcagcatcgc gcacgccggg gaatccgacg
       61 ctccacacgc tctgaccgcg cgcatcgagc aacatcaagc gactcttgcc gacggcggcg
      121 atctgcgtgc cggtcgggct cggccgcagc gtccagccgg tcatgccggt gatgcgctgc
      181 tcgccttcgg aaccgtgcac gacgatcgtg tcggcgtcga cgcgcaggta gatccggccc
      241 gcgcgatcga tcgcttcgag atttccggtc agctccgtcg gtgcaggctg cgccgcggcc
      301 agatcgcgaa tcgcgcgcac agagacggca cctttgcgcg cgcgcgtgag cagcgcgatg
      361 ttgccacccg cgagcgccgg atcgagcagc gtgacgtcac tgccgggcgc ggtgagcatg
      421 gtctcgtcgg tgaacttgcg ggtcgcagga tcgaagcgcg agaacgtgat cttgttgccg
      481 cgttcggctg ccatcagctt cgtcgccggc tcgtagtgca gcgcacccgc ggacggccag
      541 cgctggagct cggtcttgcg ctcgagatcg aacagcgcga tgcgcggcgt gctgtggccg
      601 cgaagcgtcg gtcgcgcgtt cggatcttcg agccagtcgg ggtcgatgcc attcgatacg
      661 ttgacgaccg gctcgcccca atcggtcgca gtgaacttga tcagcgcgag gtccttcgcg
      721 aacggaatcg tctccagcgc gggcacacgt ttgtcgacgc gcacgttgtc gtcgagcagg
      781 gtcgcgaccg agccgattgt cgcgacgacg ccgaccggcg agctcttcac gcctttcgcg
      841 cgactcgtgc gatagcccag aaagcgcacc tgactcggcg agctcgctgt gttcatcgtc
      901 gcgatcgcga tctcgtgacc cgagaacgcg atcacgttgt tgtcggtcac gaccgggtgc
      961 tccatcgcga cgaactcgag cgcgtagttg ctgccaccga cgacgccgac ctcgttcccg
     1021 ttcggcgtcc accactgcag ctcgctcagc tcgaagtcgt tgaacgcgaa cacgagtcgc
     1081 gatcccatga agccagccgg gaatccttcg ggcgccttgt cgtcgcgcgc gaacgttgac
     1141 gacttaccgg tctccagatc cacgagcatg tgctcgccac gcgagttgct gaacgtgacg
     1201 agccgctgat ggtccgggct caagaacacg tgctcgagat tgatcgcgag cttcggcgtg
     1261 atgtcgctcc acgcgagcgc gtcggcggcg acccagtgac cgcgcatacc ttccttcgtg
     1321 cgcacgagcg cgagcgtgcg accgccgcgg tgcagaagct tcatgacgtg ctcgccggga
     1381 ggcggcacga gcttgcccgc ctcctcgccg tgccgatcga attgcacgag cgtctggtcg
     1441 cggcggagcg cgaggaaacc gtcgccgttc tcgatcactg cgtcgaagcc cggctcgggc
     1501 gacagcttga catcggcggt gagctcgccg tttgcggtca ccgcgacgat ctcgacgcca
     1561 ccactttcgt cgcgtgctgc gatcgcgaac cgatcgccat cgcgctcgac caccgcttcg
     1621 accggcattc cgagcggtag cacccacggc tcgctcttgc cgtcgagcgt cggccatagc
     1681 cgcgcgtgat tcgattcatc gatcgagacg accgcgctgc cgtcctcggt ggcaaacact
     1741 gccgcgatcg gcacgccggg gcgcgcgtgc cacagcggaa ccggctgcgc aggcttggcg
     1801 gaagcgggag cgaccggcgc gccaaacgga agcggctcgg cgacctgcgg ggccgcacta
     1861 gacacaccgg cgggcggtgc agttgcagcg ggagccgcgt ctttctgcgg ctggttacat
     1921 gcaacgaggc aaatgggcca aacccagcgc attgtgcgtc aaacgcacga caccgctggg
     1981 ttgttctacg tttcgtcgat tttcagtagc tctttgccca cacgacgcgc tgcttgctcg
     2041 gcttgcccgt gaacgggcac gttccgggct cggcggtgat gccgagaacg ctgtccgcgc
     2101 ggtcgacgat cggaatgcac cgaacggtga ccttgaggtc gtctttgatc ttcgcttcga
     2161 gcgcggggtc accatcgaag tgcgtgagcg cgaagccggc atggatcggc gtcggatcgt
     2221 tctcccgctt cttcggcggg tcggcgaaga acgcgtagaa ctcttccttc gtgtcgatca
     2281 cccgcgtgtg ctccttgcgg aacgtacgcg cgcgctcgaa cagcgcggtc tggatctcgt
     2341 cgaggatgcc gccgaccttc gcgatgaatt cagcgcgtgg catcgacgac ttctccttcg
     2401 cgcccttgtc gcgacgcccc atgaacacgc tgtcctgatc gatgtcgcgc gggccaacct
     2461 cgaggcgaat cggcacgcct ttcttgatcc agtcccacgc cttgccgcca ccgccgacgt
     2521 cgcgatcgtc gagctgcgcg cggatcggcg cgccggcgta ctgctgcgat tcgagatctt
     2581 tcttgagcgc cttgcaccag tcgagcacgc gctggcgatc ctccggcttg cgcgtgatcg
     2641 gcatgatcac gacgtgcgcg ggcgcgagct tcggcgggca gatcatgccg tcgtcgtcgg
     2701 cgtgggtcat gatcatgccg ccgacgaggc gcgtcgacac accccacgat gtcgtccacg
     2761 cgtgctcgag cttgttgctc tcgtcggtga actggatacc cgacgccttc gagaagttct
     2821 ggccgaggaa gtgcgacgtg ccggcctgca acgccttgcg gtcctgcatc atgccttcga
     2881 tgcagtacgt cgacactgcg cccgggaatc gctcgcccgc ggtcttctcg ccttggatga
     2941 ccggcatcgc catgtactgc tcggcgaact cggcgtagac gccgagcatc ttcatcgtct
     3001 cctcgatcgc ttcgtccttc gacgcgtgcg cggtgtggcc ctcttgccag aggaactccg
     3061 cggtgcgcag gaacaagcgc gtgcgcatct cccagcgcac gacgttcgcc cactggttga
     3121 tgagcagcgg cagatcgcga tagctctgca cccacttcgc gaacgacgcg ccgatgatcg
     3181 tctccgacgt cggccgcacg accagcggct cctcgagctc gccgaccgga atcagcttgc
     3241 cgttgcgcgc ttcgagccgg tgatgggtga cgaccgcgca ctccttcgcg aagccctcga
     3301 cgtgcgccgc ttccttctcg aggaacgaca ccgggatgaa cagcgggaag tacgcgttga
     3361 cgtggccggt ggccttgaac atgccgtcga ggacgcgttg gacgttctcc cacagcgcgt
     3421 agccccacgg cttgatcacc atgcagccac ggacatcgct gttctcggcg aggtcggcag
     3481 ccttgatgac ttcctggtac cagtcctggt agttgtccgc gcgcgtcggc gtgatcgcgg
     3541 tctggggctt tgcgtcctta ctcatcgccg cggaggcata ccatctgcgc gagccaaggc
     3601 acaggcgtgg agcccctcgc gctcagcgaa tgcgcgcgat taccgcgcga tccagcgtcc
     3661 cgctcacgta gcccgtcgtc acgatggagc cgtcggcctg gagcagcaag ccctgaagct
     3721 cggtgtctcc gctcgtcggc agtgtcacca tcccgccgct tccgaagctt tgatcgagcg
     3781 tgccgtcggc ggcgagccgc acgaccacgc cactgccgtt cgctgcggtc tgtccggtcg
     3841 cgacgatctt accgttcggc tggacctcga ccgcccagaa cagcgagagc gacggcacca
     3901 tgacgaggcc gctctgcccg aagctcgtgc gcagggtccc gtccggccca gcccgcgcgc
     3961 acatcgcgac gccattgccg tcgccacacg tgacgaggtc gccgtcgccg gcgagcgcga
     4021 cgtcatacgc gttcgtgctg gcgcctccta cccgcacgaa gccgccgctt gcgaatgccg
     4081 cgtcgcgcgt accgtcgggg ctgtaggccg cgagcatcgc gcgaccagct gtgcgccccg
     4141 agacgacgag gtcgccgctc ggcatccgga tcactgcatt gcattcgtcg atacccgtcg
     4201 agaagtcatc ggtgacgacg cccgacgtcc cgaagccggc gagcggcgta ccgtcgagac
     4261 cgagctcgag caagatgatc tgcgaattca tcgcgacgta atcgtgcgtg ccgcacacga
     4321 cgaggcggtc ggcgaggaat gcgacgccgc gcgaggtgtc gtcgagttgc aggccgccga
     4381 tgtcggtgcg cgaaaagccg ttcgtcgcga acgaggaaac gagacgaccg tcggtatcga
     4441 gcaacgcgac cgtgacgttg tcgagatcgc cgctgccgtc gttgccatcg ccgacgagcg
     4501 ctgttcgccc atccgcgccg cggatcgctc cgtagccgaa gtcgttggcg gtcgggccga
     4561 ggtcggcgat gccgctcgtg ccgtacgtcg tatcgagtgc accggattgc gtgaacgcga
     4621 acacgccgaa gtaacgcgag aggctcgcgc cctcgtgaac gccgacggca aaatagccac
     4681 catccgcgcg ctcgacgacc gcataaggct gcgagccata cattcccgcc gggacgatcg
     4741 tgacaccggc ggaaccgaac gtcgcatcga gctggccgac ggcctcgaca aagggggcac
     4801 cggcgtcgcc tgcgtcagcg cgggcgttat cgcgcacgtc ctcgaagccg atgcgtccgc
     4861 acccgcacaa caggcagagg acgagcgccc gcatcgtgat cgagagtctc acgatcgcgc
     4921 tgcgagaggg cgttgatcga ccgcgagcgc cgcgtcggca agaggcctac agctcggtga
     4981 ttcgccgaac gaacttgttc ttcacgagca cggtaccggt cgcgacgacg gccgcgagca
     5041 cgtacttgtc gatcggcgac ttcggctcct gcgcggtctc gatcggcgcc ggcgcacgat
     5101 atgcggggta cggatcttct tcggggaaca cgggttcggg aatggcgtcg agcatggcta
     5161 ctacttcagg tggctagagg tgcagttggt tattgagagt tgtcgtcggt gttcttctcc
     5221 cacgagccgt acatcgagtt cgccacgagg atgaagtcct ggccgaactt tgcgtacgcg
     5281 ctcgcgggca tcttgcggat gtcctgcgtc agcgcaggga agtcgaggat gttgtcgccg
     5341 acgaacagca cgaccttcag cgccggcagc gtcgacggca tcgtgccgtt ctgcactgcc
     5401 atgaagcgcg ggttcttgtc gctcgtgtcg gtcttgcaca ggatctcgtc gtagacgatg
     5461 cccgcggcca tgaggttcgc ttcggtctgc gcgcactcgg tcgcggcgag gcggttcgac
     5521 acgaacacga ccttgccgcc gagctgcttc acgctgtgcg tgaaatcgag gacaccgtcg
     5581 agtggcgacg ccgctttgcg attcacccaa tcggtccagc tgcccggcga atagccgagg
     5641 ccgagatcga gacgctcttg ctgatactgc gagttgttga gcgtggtctc gtcgatatcg
     5701 agcacgacgc cccacggacc gctgatgccc gccgacactt tcgcctggag cttctcgatc
     5761 gcgaggctgt aggcctgcac tacgagcgcg cggcgctcgg cggagcggcg ataccacttg
     5821 aggccgaggt cggtgtcgat cggcagcgtc gtgtcacacg tcccgttgca cgccagctcg
     5881 aggcgcgcct tcttgccgtc gaccggcgtg gcggcttcgc cgaagctgac ctccgcgagg
     5941 tagaagccgg ggatcgtgat cgcgagctct ttgatcttgg acagcttgcc gtagccgctg
     6001 tcctcgtccg tcgcgagcgt cttcgggtac gtgccgtcgg cgaggcgcgg accgtagacc
     6061 ttgagcaccg tgtcgaggcc ggcgctcgag ccgccctgct tcacttcgag cgacaccttc
     6121 gtaccttcgg cggcgtagaa cacgtagccg cggcgcggca ccttcgtcga cagctgagca
     6181 tcggtcgttc ccggcttcac ccgggcggtg acctcgatac cgacggtgcc atcggccttc
     6241 tcttcgcctt ggatgtcggc tgcgtcaccc tcctcgccgg agtcagcgca agccgtcacc
     6301 agggacgcta gaagcagggc cgcaaccgtc gtcgacgcac gcatcgtgcg cgcgcccagt
     6361 gcagcgagca tgccgccgca gtgacgcggc ggtctgcccg cttacaccat tcagtgccgg
     6421 caactggagt tgccgtggcc agtcaaacct gcgaatcggg caagctttgc gtcgtcgacg
     6481 aatcaccctt tcgggcgtcg acgacgcaag cgctgcacta cgcgagaccg gcttgcttcg
     6541 cgacctcggc cgcgaagtcg tccttcttct tctcgagacc ctcacccagc tcgaagcgca
     6601 cgaagcggcg gatcttgatg ttctcgccga tcttcgcgat cagctcctgc tggagctgct
     6661 cgatggtctt gtcgggttcc ttcacgaacg gctggtcgag gagacagatc tccttcatcc
     6721 acttcgtgac ctgaccctcg gtgatcttcg cgaggatggc gtcgggcttg cccgccatct
     6781 tcgggtcttc cttcgccttc gcgatgagga cggccttctc gtgctcgacg gcctccgccg
     6841 gaacctcttc cttgcgaacg tactgcggcg ccatcgcggc gatctgcatc gcgacatcgc
     6901 gggtgaacgc gacgaagtcc tcgttgcgcg cgacgaagtc ggtctcgcag ttgatctcga
     6961 cgaggacgcc gatgcggccg ccgccgtgga tgtacgaaac gacgaggccc tcggtcgctt
     7021 cacgaccggc cttcttcgct gccttcgcga gacccttcgc gcggaggtag tcgatcgcct
     7081 tgtccatgtc accactgact tccgtcagtg ccttcttgca gtccgacatg ccggcgccgg
     7141 tgcgctcgcg caggtctttg atcatcgttg cgttgatttc catcgcagtt actcgccttc
     7201 cggagatgcc gaggcctcgg ggttgaacat gctgccgccc gtcgacatct cgacgcgcgg
     7261 accatcgcca cccgacgaca cgtggatcac gcgctcgccc tcttcctgac gctcctgacc
     7321 acgatgacgc gcggtctcct tccagacctt ctggccgatg atcgcggcgt cggcgatgcg
     7381 ggctgcgaac agcttgatcg cgcggatcgc gtcgtcgtta cccgggatga cgtagtcgat
     7441 ctgatccgga tcgcagttcg tgtcggtgat cgcgacgacc ggaatctcga gcttgcgcgc
     7501 ctcgtcgacg gcaatgtgct ctttgtgcgg gtcgatcacg aacagcatgc cgggcagctt
     7561 gctcatgcct ttgatgccgc cgagcgactt gagcagcttg tcgcgatcgc gcttgatcat
     7621 cacttgctcg cgcttcgtga gcgcgaacag cgtgccgtcc tcttccatct tctcgagcga
     7681 gcggaggcgc tcgagcgagc ccttcaccgt gcggaagttg gtgagcgtgc cgccgagcca
     7741 gcgctgcgtc acgtagtgct ggttgctgcg cgtcgcctct tcgacgatca cttcctgcgc
     7801 ctgcttcttc gtgccgacga acaggatcgt gtcaccgctg ctggtcgtct cgaccacggc
     7861 gttgaacgcc tggcggaaca gcttgaccgt gtgctgcagg tcgatgatgt ggatgccgtt
     7921 gcgcgcgccg aagatgaact gcttcatctt ggggttccaa cgacccgtgt tgtgtccgaa
     7981 gtgcacgccc gcctcgagaa gggcgcgcat gctgatgggg ttttgccccg tcgcttctgc
     8041 catcgtttcc atgtcaatca ccttttggtt gcgtagccac ctgcaccgtc atcgatgacc
     8101 ggaaaccctc gaaggggcca ccgccggaaa tctcggatgc agtgagtagt ccccggattc
     8161 attccggagc gccgaggatc tagcccatct cgcggccggt tacaaggctc ggtgaatccg
     8221 atgcgaggcc ggtttggaca tgtcatccat cgtcgcggaa acgatggat
//
LOCUS       DASUFX010000097         5475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_293560,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000097 DASUFX010000000
VERSION     DASUFX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293560"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFV99_03775"
     CDS             complement(<1..446)
                     /locus_tag="VFV99_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058450.1"
                     /translation="MTTPRDQLDLECEIVYLRALIAIAARDRNEDDAQRLRQQLATAG
                     ALHDGWERTGAVRTTPMARLVERYALTPLETELVWAIIATSADPRLAIPLEALWGAGS
                     RRGISVAAFTHLRGVPSDAGRDLALWLAQRSALVAGGLIVATDTSAP"
     gene            complement(443..1267)
                     /locus_tag="VFV99_03780"
     CDS             complement(443..1267)
                     /locus_tag="VFV99_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058451.1"
                     /translation="MRITVGLAVLALASCAKTPAEHTVARPIDATQVVIAPDKASPPP
                     AAPAPAPAPAATDDKQCVIAINTLMRDIAGTYAAMDSATPHKKAVEAWPAVPATCRNG
                     RWYLAAALLIDAGDKDLTAGTIKVASEEAALTSALAQPDDIDVLVRVGLVNGLGRGPK
                     LPADACDRAKAAVAGSTKGDDADKAAYVCARAAIAANDGKAAGQLLASLKVPKLFVDS
                     ELARAQAAKLAKDSKTTKAQAKLAMKLDSTRVAAAKVSEADRKAIIAAAKQLAAGK"
     gene            complement(1277..2779)
                     /locus_tag="VFV99_03785"
     CDS             complement(1277..2779)
                     /locus_tag="VFV99_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX5058452.1"
                     /translation="MRESAPRHEQGLAEGAREIHMEPSPELQRLARTVVSDQAQHGQA
                     DAGTLTTSIDLPFREEMEARFGQSFAQVRAHVDPAAAAAVGARAYTLGTDIVFATATP
                     DRELVAHELMHVVQQMHGTSSDVASDEAQAETAAQGGDVAPAAQAAGGAPTRALRKDG
                     ILNAQQIKAAIDFNNSHWKDPHRAEILKTLRVEASAAEFVDTDVIKIASMQQTEGVDP
                     KAVDGKLGPTSMAILLRKGLHLSEIAAKAEDVRLVFYPGEFENLKAWAEARDKAKADA
                     AKPGGGGREAEFGHMYNYAPKDGHGTIYVYYKGNVVDAIQARGGPPFTMHDRGHTADP
                     SQAGTYSLGKGASVVTKSWNTSQIAWGAPLREVDGYIQFKNPGQDWQWATGPKAKIDP
                     IPDEYFREPDGSLSPTYKNNDFGETAFRVEGSPGLYVHTGPETEATLRQEQEEHKVHE
                     SMQLSHSHGCLHVDPYDRNRLMKSGYLQKGVTMVIKKYEDALDPGALNKK"
     gene            complement(3030..3932)
                     /locus_tag="VFV99_03790"
     CDS             complement(3030..3932)
                     /locus_tag="VFV99_03790"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5058453.1"
                     /translation="MTDWVKPLPQHHGAHGGLMALGLLVASGIVVWATHPRQSIPPVS
                     EAPPAAHAPPVPTPLPAGRLAETPSEPVIVAPLPTRVTATGFVSFDERRTTHIGVPVA
                     GLFEKKRATSVGRWIRQGETLGTIYSPEVYLTTVDLLGELRTFTSQENVDRERIRLLR
                     WGMPRPTLAAIEKKMTPQLALPLVTRTAGIVVAEQGESRRMIDPSSRFELFTITEPAY
                     TWVFVQVPDADAARTQPGTPAKLTVEGITRPIAAKVAYVFRRSEEGKRTVRFDVYSPR
                     VMLPPNARVSAELQLSATPAPAKQ"
     gene            4072..4716
                     /locus_tag="VFV99_03795"
     CDS             4072..4716
                     /locus_tag="VFV99_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058454.1"
                     /translation="MHSRWKVGIAVALTVSCSGSPQRSDKPTVAQRPIGNQAAALAWC
                     DQDYGHVPCAFTAFGGCAEKVGKGRTIIDDDFGPTTCPAGTPPPFMTKQQPVCGRRAT
                     ETVIAKKSQFTYEASCNQRETTWANVAHEHRTCASDADCVVVPFNAQCDTFPLAKSAA
                     TRNEYAQGPCGNPAQGFCAGQLPAAKCVAGCCNLDRAIDRWGTLPVEYPKLALP"
     gene            4906..>5475
                     /locus_tag="VFV99_03800"
     CDS             4906..>5475
                     /locus_tag="VFV99_03800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058455.1"
                     /translation="MIAPGDTLADRFELRRRIGSGGMGEVYEAFDRDTAEVVALKTLA
                     HADGDTVTRFKREFRALQSTSHPNLVGLRELIRDGDHWFLTMELVNGRHFLEHVRGDA
                     DKLRAALRQLVQGLRVLHESGLIHRDIKPSNVMVSNEGRVVLLDFGLVTALDPARQSI
                     DGRATGTVEYMAPEQGGFGRPVTEAADWYA"
BASE COUNT          856 a         1840 c         1760 g         1019 t
ORIGIN      
        1 ggtgccgatg tgtcggtcgc gacgatcagc ccgcccgcca cgagtgcgct gcgctgcgcg
       61 agccacagcg cgagatcgcg cccggcatcc gacggcacgc cgcgcagatg ggtaaacgcg
      121 gcgaccgaga tgccacgccg actgcccgcg ccccacagcg cttcgagtgg aatcgcgagg
      181 cgcggatcag cagaggtcgc aatgatcgcc cagacgagct cagtctcgag cggagtcagt
      241 gcgtaacgtt cgacaagacg ggccatcggc gtggtccgca cggcgccggt tcgctcccaa
      301 ccgtcgtgta acgcacctgc tgtcgcgagc tgctgccgga ggcgttgcgc gtcgtcttcg
      361 ttgcgatcac gcgctgcgat cgcgatcagc gcgcgcaggt acacgatctc gcattcgaga
      421 tcgagctgat cgcggggtgt cgtcacttgc cggccgcgag ttgcttggca gctgcgatga
      481 ttgccttgcg atccgcttcg ctgactttcg cggcagcgac gcgcgtgctg tcgagtttca
      541 tcgcgagctt cgcctgtgcc ttcgtcgtct tgctgtcttt cgcgagcttc gctgcctgcg
      601 cgcgcgcgag ctcgctatcg acaaacagct tcggcacctt caggctcgcg agtagctgac
      661 cggcggcctt gccgtcgttc gctgcgatcg cggcacgcgc gcacacgtag gcagccttgt
      721 cggcatcgtc gcctttcgtg cttccagcga ccgcggcctt cgcgcgatca caggcatcag
      781 ccggcagctt cggcccacgg ccgagtccgt tgacgagtcc gacgcgaacg agtacgtcga
      841 tgtcgtcggg ttgcgcgagc gcactcgtca gcgcggcctc ttcgctggct actttgatcg
      901 tgcccgcggt cagatcctta tcgcccgcat cgatcagtag cgcagcggcg agataccagc
      961 ggccgttgcg gcaggtcgct ggtaccgcag gccatgcctc gaccgccttc ttgtgcggcg
     1021 ttgccgagtc catcgcggcg taggtgcctg cgatgtcgcg catcagcgtg ttgatcgcga
     1081 tcacgcactg tttgtcgtcg gttgctgccg gcgctggcgc tggcgctggc gccgcaggtg
     1141 gcggcgatgc tttgtcgggc gcgatcacga cctgggtggc gtcgatcggg cgcgcgacag
     1201 tgtgctcggc cggtgtcttc gcgcagctcg cgagcgcgag cacggccaac cccacggtga
     1261 tgcgcatagc tcgagcttac ttcttgttga gcgcgcccgg atcgagcgcg tcctcatact
     1321 tcttgatgac catcgtgacg cccttctgca ggtagccact cttcatcaag cgattgcgat
     1381 cgtacggatc gacgtgcaag cagccgtggc tgtgcgacag ctgcatcgac tcgtggacct
     1441 tgtgctcctc ttgttcctgc cgaagcgtcg cctcggtttc gggaccggtg tgaacgtaca
     1501 aacccgggct gccctcgacg cgaaacgcgg tctcgccgaa gtcgttgttc ttgtacgtcg
     1561 gcgatagcga accgtcgggc tcgcgaaaat actcgtcggg aatcggatcg atcttcgcct
     1621 tcggtccggt ggcccactgc cagtcctggc cgggattctt gaactggatg tagccatcga
     1681 cttcacgcag cggcgcgccc caagcgatct ggctcgtgtt ccagctcttg gtcacgaccg
     1741 acgcgccctt gccgagcgag tacgtgccgg cctgtgatgg atcggcggtg tgaccgcgat
     1801 cgtgcatcgt gaacggtgga ccgccgcgcg cctgaatcgc atcgacgacg ttgcccttgt
     1861 aatagacgta gatcgtgccg tgcccgtctt tgggcgcgta gttgtacatg tggccgaact
     1921 cggcctcgcg gccaccgccg ccgggcttcg ctgcatcagc tttcgctttg tcgcgcgcct
     1981 cggcccacgc cttgaggttc tcgaactcgc ccggatagaa cacgaggcga acgtcctcgg
     2041 ccttcgcggc gatctcgctc aggtgcagac ccttgcggag caagatcgcc atcgacgtcg
     2101 gaccgagctt gccgtcgacc gcctttggat cgacaccttc cgtctgctgc atcgatgcga
     2161 tcttgatgac gtcggtatcg acgaactccg cggccgacgc ttcgacgcgc agtgtcttca
     2221 agatctccgc gcgatgcgga tccttccagt gtgagttgtt gaagtcgatc gccgccttga
     2281 tctgctgcgc gttgagaatg ccgtccttgc gcagcgcgcg tgtcggcgcg ccacctgcgg
     2341 cctgcgcggc cggcgccacg tcgccaccct gtgccgccgt ttctgcctgc gcttcgtcac
     2401 tcgcgacgtc gctgctcgtg ccgtgcatct gctggacgac gtgcatgagc tcgtgcgcaa
     2461 cgagctcgcg atccggtgtc gccgtcgcga acacgatgtc cgtgccgagc gtgtacgccc
     2521 gcgcaccgac cgctgcggcc gccgcgggat cgacatgcgc gcgcacctgc gcgaacgatt
     2581 gcccgaaccg cgcctccatc tcctcgcgaa acggcagatc gatcgacgtc gtcagggtcc
     2641 cggcatccgc ttggccgtgc tgcgcttgat cgctcacgac cgtgcgcgcg agccgttgca
     2701 gctcggggct cggctccata tggatctcgc gcgcaccctc tgccaaccct tgctcgtgcc
     2761 gtggcgctga ttcccgcatc cgtccccatt ctacgtaacc accgggaccc agtcgccctt
     2821 gccagttcac ttggcgacgc gcccacttat ccatgacagc catgcgtcct cgccttcgca
     2881 ggtgcgacgc gaatcgcggg caacgccgtg tcacacggcg ctagctcgcc ccgctggcgc
     2941 accgaggcac gatcgatcga cagctagggc atgagctcac gtgcgtggtg gttggtcctg
     3001 ctgctcggtt gcgacgtccc cgcgctagct cattgcttcg ccggagccgg cgtcgccgac
     3061 agctgcagct ctgcgctcac gcgtgcgttc ggcggcagca tgacgcgtgg gctgtagaca
     3121 tcgaagcgca ccgtgcgctt gccctcctcg ctgcgccgaa agacgtacgc gaccttcgcc
     3181 gcgatcggcc gtgtgatgcc ctcgacggtt agcttggccg gcgtgcccgg ctgcgtgcgc
     3241 gctgcatccg catccggcac ctggacgaac acccacgtgt acgccggctc ggtgatcgtg
     3301 aacagctcga agcgactcga cggatcgatc atccgtcgcg actcgccctg ctctgcgacg
     3361 acgataccgg cggtccgcgt gacgagcggc agcgcgagct gcggtgtcat cttcttctcg
     3421 atggccgcga gcgtcggtcg tggcattccc caccgcagca gtcgaatgcg ctcgcgatcg
     3481 acattctcct gcgatgtgaa cgtgcgcagc tcgccgagta gatcgacggt cgtcaggtag
     3541 acctctgggc tgtagatcgt cccgagggtc tcgccttggc ggatccaccg gccgacggac
     3601 gtcgcgcgtt tcttctcgaa cagccccgcg accggcacgc cgatgtgcgt cgtgcgtcgt
     3661 tcgtcgaagc tgacgaagcc ggtcgcggtc acccgcgtcg gaagcggtgc cacgatgacg
     3721 ggctcactcg gcgtctcggc cagcctgcca gcgggcagcg gcgtcggcac cggtggcgcg
     3781 tgagctgccg gcggcgcctc gctcaccgga gggatcgact gacgcgggtg cgtcgcccac
     3841 accacgatgc cagaggcgac gagtaggcca agcgccatga ggccaccatg tgcgccatga
     3901 tgctgcggca atggtttcac ccaatcagtc atgaccgacc tcgcctgttg tctttaccga
     3961 cgagcgtttc aaggcgcatg cctgcgcagg tgacggaaga cggcgacaaa gtccgcaacc
     4021 cgtgcgccac gcggccggag cctgcagtgc ctgcgcggta cgcttggcct gatgcacagc
     4081 aggtggaagg tcgggatcgc agttgcgctc acagtttcat gctcggggtc gccgcagcgc
     4141 tccgacaaac cgaccgtcgc gcagcggccg atcggcaatc aggccgcagc actcgcgtgg
     4201 tgcgatcaag actacggtca tgtgccatgc gcgttcaccg cgttcggcgg atgcgccgaa
     4261 aaggtcggca agggccgcac gatcatcgat gacgatttcg gaccgactac gtgtccagcc
     4321 gggacgccgc cgccattcat gaccaagcag cagccagtct gtggccgccg tgcaaccgaa
     4381 acggtgatcg cgaagaagtc ccagttcacg tacgaggcaa gctgcaatca acgggagacg
     4441 acgtgggcga acgtcgcgca cgagcaccgc acctgcgcga gcgacgcaga ctgcgtcgtg
     4501 gtgccgttca acgcacaatg cgacacgttt ccactcgcga agagcgcagc gacgcgcaac
     4561 gagtacgcgc agggcccgtg cggcaaccct gcgcaggggt tctgcgctgg acagcttcct
     4621 gccgcaaaat gcgtcgctgg ttgctgcaat ctcgatcgcg cgatcgatcg atggggcaca
     4681 ctccccgtcg agtatccgaa gctcgcattg ccctgaccac tcgcgggctg gggatcaagg
     4741 tgccagctcc gaattcctcg cgtgtgtggt aattagcctg gaagacgcac agctcgacga
     4801 gtggtttgat cgcaaaccat ctcgtccacg tctggagcta tcgaatcgcg atggccgccg
     4861 cgatatcgtt tactgagaag tgaacgatca accgcgtgga gtcaggtgat cgcgccgggc
     4921 gacacacttg cggatcgatt cgagctgcga cgccgaatcg gatctggcgg gatgggcgaa
     4981 gtctacgagg cgttcgatcg cgacaccgca gaggtcgtcg cgctcaagac gctcgcccat
     5041 gcggacggtg acaccgtcac gcggttcaag cgcgagttcc gcgcgctgca gtcgacctcg
     5101 catccgaacc tggtcggcct gcgcgagctg attcgcgacg gcgaccactg gtttttgacg
     5161 atggagctcg tcaacggccg ccacttcctc gagcacgtcc gtggcgacgc cgacaagcta
     5221 cgcgccgcgc tgcgccaact cgtccagggc ttgcgcgtcc tccacgagag cggcctgatt
     5281 caccgcgaca tcaagccgtc gaacgtgatg gtgagcaacg aagggcgcgt cgtgctgctc
     5341 gactttggct tggtgacggc cctcgatccg gcccgccaat ccatcgacgg acgcgcgacc
     5401 ggcaccgtcg agtacatggc acccgagcaa ggcggatttg gtcgtccggt caccgaggcg
     5461 gctgattggt acgcc
//
LOCUS       DASUFX010000098         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1545402, whole genome shotgun sequence.
ACCESSION   DASUFX010000098 DASUFX010000000
VERSION     DASUFX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545402"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFV99_03805"
     CDS             complement(<1..852)
                     /locus_tag="VFV99_03805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5058456.1"
                     /translation="MSDTLLSKTIASTATLKIANINSLDLFYTPIEERFERITRIARR
                     ALHAPVAAISLLNEDKQWFKSAAGWGISELPRDQAICKLTVEANQIVTINDTLLDPRV
                     AKLPIVMAAPRFRAYAGHPLVDDEGNCVGTFCVFDLKPREFAPVDRQALIDMAALVQR
                     ELFSEQLSSAHAALTSKLGMARREALMDPLTHLWNRRGASVLLKAAFARADQRGTPLA
                     LALLDLDNFKRINDTRGHQAGDEVLRRVASRLLSTVRGDDAICRLGGDEFLILMADTD
                     AAIASRIA"
     gene            1055..>2533
                     /locus_tag="VFV99_03810"
     CDS             1055..>2533
                     /locus_tag="VFV99_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058457.1"
                     /translation="MLQRKTRKEAADGRAHLEVAALESSSVVAAIAVAADGAILTANA
                     RMRRFLGLHGGDVEKASFRAHLADAAAWATWRDAARASRPIELELRGVDGTTKRFRGD
                     VLLIGDGAEQRFVGVLVDGDDSKALRAALQHSARMDALASLTAGVAHDFNNLLTVLVG
                     NLYLIGEELRERPRVFEKLKAARDAGKRGSELIKQLMTFARREELEVGTIDPSKVIGD
                     LLPLLRRALGPRITLETALQKHVGTVRASVAQLESVVVNLAVNSRDAIEGKGRISIDV
                     SAVEILDHDAALRGLARGGRYVAVSVRDTGMGIASDALEQVFEPFFSTKRERGGTGLG
                     LSMVRWFAENAGGCAAIESVVGRGTTVTLLLPRQQGDQAAETAEMTMPLSTLPTGTER
                     VVVLAMDDALRATIHQTLEVLGYGVTVAGNTEDLLAAVAATPTDLLVIDGLGRGDEVL
                     ARARALRPTLRIIATAHPGRTGEQAAGLDIASLTKPFTLADLA"
BASE COUNT          441 a          806 c          862 g          424 t
ORIGIN      
        1 cgcgatgcgg ctcgcgatgg cggcatccgt gtcggccatc aggatcaaga actcgtcgcc
       61 accgagccgg caaatcgcat cgtcgccgcg gaccgtgctc aacaggcggc tcgcgacacg
      121 tcgcaggacc tcgtcgccgg cttgatggcc gcgcgtgtcg ttgatgcgct tgaagttgtc
      181 gagatcgagc aaagccagtg cgagcggcgt tccgcgctgg tccgcgcgcg cgaacgcggc
      241 cttgagcagc accgacgccc cgcgtcggtt ccacaaatgc gtgagcgggt ccatcagcgc
      301 ttcgcggcga gccatgccga gcttcgacgt caaggcagcg tgcgcactcg acaactgctc
      361 ggagaacagc tcccgctgca cgagcgcggc catgtcgatc aacgcttgcc tatcgaccgg
      421 cgcgaactcg cgcggcttca gatcgaagac acagaacgtg ccgacgcaat tgccttcatc
      481 gtcgacgagc ggatgaccgg cgtaggctcg aaagcgcggc gccgccatca cgatcggcaa
      541 tttcgcgaca cgcggatcca gcagagtgtc gttgatcgtg acgatttgat tcgcctcgac
      601 cgtgagcttg cagatcgctt ggtcgcgcgg cagctcggag atgccccaac cagccgccga
      661 cttgaaccac tgcttgtcct cgttcagcaa cgagatggcg gcgaccggtg cgtgcagcgc
      721 acggcgcgca atgcgtgtga tccgttcgaa acgctcctcg atcggagtgt agaagaggtc
      781 gagcgaattg atgttcgcga tcttgagcgt ggccgtgctc gcgatggttt tgctgagaag
      841 ggtgtcgctc acggggctcc gggccggcca actgacgcct gaggatggac cctaatgtaa
      901 ctcgcgagtc agcctacggc gtgtgagttg cgtctcgctt gcaatggccc ctagggacgg
      961 ccgtgcgcgg taaagtgtga cgtcagtcaa cagcgtgcgt ggcgcaagta gttagcctgt
     1021 caatacacct gccgctccgc cggtcggaga aacgatgctg cagcgaaaaa ctcgcaagga
     1081 ggccgctgat gggcgcgcgc acctcgaggt cgcggcgctc gaatcgagca gcgttgtggc
     1141 tgcgatcgcg gttgcggccg acggcgcgat tctcaccgcc aatgctcgca tgcgccgctt
     1201 cctgggtctt cacggcggcg atgtcgagaa ggcatcgttc cgcgctcacc tcgcggacgc
     1261 cgccgcgtgg gcgacttggc gcgatgccgc gcgcgccagt cggcccatcg agctcgagct
     1321 gcgcggcgtc gacggcacga caaagcgttt tcgcggcgac gtgctgctga tcggcgatgg
     1381 cgccgagcag cgtttcgtcg gcgtgctcgt cgatggcgac gacagcaagg cgctacgtgc
     1441 cgcacttcaa cacagcgcgc ggatggatgc gctcgcgagc ttgaccgcgg gcgtcgcgca
     1501 cgacttcaac aatctgctca cggtcctcgt cggcaatctc tatctcatcg gagaagagct
     1561 ccgcgagcgg ccgcgggtat tcgagaaact caaagcggcc cgcgacgcgg gtaagcgcgg
     1621 gtccgagctc atcaagcagc tcatgacgtt cgctcggcgc gaggagctcg aggtcggcac
     1681 catcgatccg tcgaaagtga ttggcgacct gctgccgctg ctgcgccgcg ccctcggccc
     1741 gcggataacc ctcgagaccg cgttgcagaa gcacgtcggc acggtgcgag cgagcgtggc
     1801 acaactcgag agcgtcgtgg tcaatctggc cgtcaattca cgcgacgcga tcgaaggaaa
     1861 ggggcgcatc tcgatcgacg tgtcggcagt cgagatcttg gatcacgacg cggcgctacg
     1921 cggactcgcg cgcggtggac ggtacgtcgc cgtcagcgtg cgcgataccg gaatggggat
     1981 cgcgtcggac gcgctcgagc aggtgttcga gccgttcttc tcgacgaaac gcgagcgcgg
     2041 cggcactggc ctcgggctca gcatggttcg atggtttgcc gagaatgccg gtggttgcgc
     2101 cgcgatcgag agtgtcgttg gtcggggaac gaccgtgact ctgctgttgc cgcgccagca
     2161 aggcgaccag gcggccgaaa ctgcagagat gacgatgcca ctttcgacgt tgccaaccgg
     2221 cacggagcgc gtcgtcgtgc tcgcgatgga cgacgcccta cgcgcgacga tccatcagac
     2281 gctcgaggtc ttgggctacg gagtcacggt cgcggggaat accgaggatc tgctcgcggc
     2341 cgtcgcggcc acgccgaccg acctgctggt aatcgacggc ttaggacgcg gcgatgaggt
     2401 gctcgcccgc gcacgggcgc tacgcccgac actacgcatc atcgcgacgg cccatccggg
     2461 ccgcaccggg gagcaagctg ccgggctcga catcgcgtcg ctgacgaagc cgttcacgct
     2521 ggcggacctc gcg
//
LOCUS       DASUFX010000099        14069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1266468, whole genome shotgun sequence.
ACCESSION   DASUFX010000099 DASUFX010000000
VERSION     DASUFX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14069
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266468"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..362)
                     /locus_tag="VFV99_03815"
     CDS             complement(18..362)
                     /locus_tag="VFV99_03815"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX5058458.1"
                     /translation="MIIRILLLLGIAATGYLIFLRRNRLPLHIMTVFGLLAAGAAAVI
                     DPNLADDVAHMVGVGRGADFIFYISITGVLFVLMHYYTKFVEMQQQITDLTRELAILR
                     AEVDRTSKPTDT"
     gene            complement(359..1033)
                     /locus_tag="VFV99_03820"
     CDS             complement(359..1033)
                     /locus_tag="VFV99_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5058459.1"
                     /translation="MTEQDRRGTWVVIAAFNEGKVIRDVVSSVVRDGWPVVVVDDGSS
                     DDTATAARVPGAIVVRHAINRGQGAALQTGIDYALRRGAKHIVTFDADGQHDAGDIPT
                     LIDALGEADIALGSRFLGKVEGASSSRMAMLKTATVVSNSLSGMKLTDSHCGLRAFRA
                     SIAEKLRITQDRMAHASELLRKIRTSKLNVVEVPVTIRYTEHSMSKGQGGIQAVRILF
                     DYFFRA"
     gene            complement(1564..1866)
                     /locus_tag="VFV99_03825"
     CDS             complement(1564..1866)
                     /locus_tag="VFV99_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058460.1"
                     /translation="MRAVPLAGLVDELGCVLDVRRLLSRPDVVRDQKEARLLHVDVHA
                     SVDRVEDRLHRADLVLPGQLVAEIELVRFPDCGERPGEVTGAAGDAIDVTSESERR"
     gene            1928..2074
                     /locus_tag="VFV99_03830"
     CDS             1928..2074
                     /locus_tag="VFV99_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058461.1"
                     /translation="MFDVVEPYFANNLAQGRDATCNVHDNFFGVDVDVEAGLLPEYRD
                     LLDD"
     gene            2103..3086
                     /locus_tag="VFV99_03835"
     CDS             2103..3086
                     /locus_tag="VFV99_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5058462.1"
                     /translation="MPRVAIAMPVYNDEQYLALALEGLCRQTDRDFALTVVDDGSTDA
                     SAAVAESFADRLPITVIRAEHRGLRTTKRAAVEASSPDAEFVLMHDADIELPDDAIER
                     MVAMLDSDANVAAVSATARSVPSRRWGRGQAFFEYLVRHSLVNDRDESRGIVGGCVML
                     RRTALAGIRLRVDINEDVDLSLQLRNRFKLMWPRDLVADHWGVPTTLRGIWNRGYRDG
                     LRVRAIMRVHPKDFQLGTVARLVPLPLSAAMAIGALTLQPWLVGGAATVLAAYTAAFL
                     YASRHVPGDLGTRLEGTALFLLSNFGFAAGFVRESLSKPTDEELSEPPRTG"
     gene            complement(3095..4285)
                     /locus_tag="VFV99_03840"
     CDS             complement(3095..4285)
                     /locus_tag="VFV99_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058463.1"
                     /translation="MTTIVDIVNFNADASCLSSGAWLQALAGGEQSAVCSWLRLYVEL
                     RKKVMLGFTGATVADMRAFNPEAIDLVNAHPDIFESIARPFSHDISLLRSREGFLINL
                     DLGWRYLQREFNRVHPLYLPPEFMLTNEQVSLLVERGFSAVTVNAARFSAELQQRIPR
                     EPYVLKGIAGAQLRCLPVRGALTQAYLDSIHELDAAPWANVLRDNGVPLLGSWRDGES
                     PFLIPDGVARERAWLATEPPLERAHLSDLAVEYRPHEELSAEAYHSYPVHSFTAWMKE
                     FRMLGYVSRLQQLEQHVSSFDATELALWLCAINSDVLSAVEKASPTVNLKKARGETAT
                     FPFVIYRSERGLEGEEYLVLLEGLRRAGSARDYIDRFLASGQPHAKKLAARITAIRGS
                     LRGV"
     gene            complement(4282..5319)
                     /locus_tag="VFV99_03845"
     CDS             complement(4282..5319)
                     /locus_tag="VFV99_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5058464.1"
                     /translation="MRRFDAEKLRATMTRVLGARGVAPEAVAHVVASVIQTSLRGVDS
                     HGIQLFPHYARVAETGRINLAPKLAFEQTAPGTAILDADHAYGHHAGAVAMDHAIQCA
                     KQVGIAVVAVRNSSHFGAAAYFALRAPAAGCIGLAFTNADALVKATGAKHSFTGTNPI
                     CFTAPIEGEQPFCFDAATSLVSWNKVVNARRTGGSLGEDWACDENGLPVTDANAARSL
                     QPAGLYKGYGLSIVVDILCALLAGGGISKDLLPMYKHVEVRRAISHCFMAIDVARFAA
                     LGDFAHRMKDMVERARGLEPLDPAAPVMVPGDPEKRAFAERTARGIPIDDAKLAEFIA
                     TSADFESALLP"
     gene            complement(5306..6475)
                     /locus_tag="VFV99_03850"
     CDS             complement(5306..6475)
                     /locus_tag="VFV99_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5058465.1"
                     /translation="MKVLVVGAGMYVTGRHGTGAGTVMAALAQASRTMPIDEVLVVAR
                     DPGNAALVTEAAARVNQLLGSSLAFRYQVIDGDRRLDQQLSLADFACSIVVVPDHLHF
                     RYTAELLEARVPPLVVKPLVPTVAEARKLIEIQDRTDTYAAVEFHKRWDESNLMTRRY
                     IAENRLGPLVYAVVDYSQRIRIPTEVFKGWADRTNIFQYLGVHYVDLLYFLTGLTPLR
                     AMAVGTRGVLTSRGVNTYDSVHAQVLWGKPGDAEHRFVSLLSINWIDPNTTSALSDQK
                     MKVICGEGRIELDQKDRGIEVVTNRDGIQHVNPYFADYLPSDSGSLDFQGYGYKSIAA
                     FFADVLALQAGKTTRAKLEPLRPTLRQSLVSVAIVEAVNESLAHGSEWRTIDASL"
     gene            complement(6472..7182)
                     /locus_tag="VFV99_03855"
     CDS             complement(6472..7182)
                     /locus_tag="VFV99_03855"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEX5058466.1"
                     /translation="MSNVVAIIPARGGSKGIKRKNLVPLAGKPLVAHSVQHALDAKLV
                     DRVIVSTEDAEIAEAARAAGAEVPFLRPDELAGDLVLDWPVFEHALRWLEAHGDKPEV
                     VVHLRPTAPYRKAGWIDEAVELLRANPNADSVRSVSKVDQHPWRVFKIGDDGVLDPLM
                     KAEHPQPYLLRRQDLPPLYYYNCVIDVTRRSTILDKQSMTGDRMLAYVMNANDVLDID
                     TPRDLAFARFVLEGGGRS"
     gene            complement(7179..8168)
                     /locus_tag="VFV99_03860"
     CDS             complement(7179..8168)
                     /locus_tag="VFV99_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058467.1"
                     /translation="MATRHFCTFFDKNYLSRGLALYESLRAHAGDFRWYVLCLDDEAH
                     RYLSERALPEVVLVKRSELEAADPALAATRPTRSLVEYYFTITPAWVCYVLATYDLDI
                     VTYVDADFRFFSSPEPLFTELGDKSVGVVEHRFPPARAHLEKWGRFNVGWLSFRNDEQ
                     GKACAAWWRERCIEWCYDRIEGDKFADQKYLEQWPRLFSQLAILQHKGVSVAPWNLEP
                     EKLRIDAGRTMIDDQPLICFHYHGFKHVVGPLYETGLRAFGLRLDDTLRTALFAPYLT
                     ELLSHEARLARAGITKGHAKTLRLRNGGLGDLRRRATNAVGAIVKRTGLWART"
     gene            complement(8168..8962)
                     /locus_tag="VFV99_03865"
     CDS             complement(8168..8962)
                     /locus_tag="VFV99_03865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5058468.1"
                     /translation="MADPRVSFMIPTRNHAQFIRTCIDSCLAQQLPSYEIVVMDGAST
                     DNTVEVLKSYGDKLRFVSEKDSGQSDAINKGIKAARGEIIAWINSDDYYPNGDVLRRV
                     LAKFDEAPDVDIVYGHGMMVDIEHKPLKRFEAYQINALKEMAIHGRCFVAQPAVFFKR
                     QLFLDVGGVAEGLHLAMDYDLWMRLFPAARSRRFLDEDLACIVSHQDAKSVHSVWKQI
                     REMHRIKRRYIPKLGLDAKDWVKLAMGEAVIYTYGIAVRTGLWKVT"
     gene            complement(8955..10043)
                     /locus_tag="VFV99_03870"
     CDS             complement(8955..10043)
                     /locus_tag="VFV99_03870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5058469.1"
                     /translation="MTIPMNDFVRQVATMRRELDEAFARVLDSGWFILGKEVEAFERE
                     MAAFAGVSHAIGVGNGMDAIELALRACDIGAGDEVITTTNSAFATALAIVRAGATPVF
                     ADIDPQTYSIDPARVEALITPRTRALLPVHIYGQAADLTALETIATRHKLTLIGDAAQ
                     AHGATHDGRDVATFGAATCYSFYPTKNLGAFGDGGMVVTNDAKLAERVRRLRNYGQTN
                     RYEHADLGLNSRLDELHAALLRVKLRHLPAHNARRRAISARYRATLADLPVQLPVEKH
                     GESVWHLFVVRTPKRDALGQFLREHGITTHVHYPIILPKQPALALAGSWPVAEQCAQE
                     YLSLPIFPELTDDEIDTVCARIAEFFRG"
     gene            complement(10040..11089)
                     /locus_tag="VFV99_03875"
     CDS             complement(10040..11089)
                     /locus_tag="VFV99_03875"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5058470.1"
                     /translation="MTVQELPAIPELVSRRDDVRAYYSGRSVLVTGGAGFIGSNLART
                     LVALGAKVTVLDNLAPNYGGNRFNLGGVELRFIEGDQTDQREMAKLVRDFDSVFNMVG
                     QVSHVDSMEDPLTDLRTNVMTHIGLLEALRKERPDVKVVFAGTRGQYGRTAGLVDESA
                     HIAPIDVNGINKHAGETYHFVYARAYGLRVTSLRLTNTYGPRHTMRTARQGVLAWFVR
                     QALDGQAIRLFDGGEQLRDCNHVSDVVAALLIAMVSPAADGEVFNLGSKKPISLREIA
                     QTIVAQAKSGSVTNVPYPPHLKSIEIGDYVGDYTKAHRVLGWEPAIDLARGMADTVEY
                     YREHREHYWTHEAYP"
     gene            11186..12028
                     /locus_tag="VFV99_03880"
     CDS             11186..12028
                     /locus_tag="VFV99_03880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5058471.1"
                     /translation="MFECRNCKAHRAEPIYHQLRDRFEGHPGTFDYVRCGECSLVQLA
                     EIPPDLEALYMGYRVHGRTSSLYHLLRKLTIGHAYLSTSGEGRSLLDFGCGSGWYLQQ
                     MAELGWQATGYESNADHAAHVSRVTSLPVLSGLDSLKARTGEFDLVTMNFVFEHLADP
                     HEMLDLVSRVLRPGGQLYISVPNIESREARIFKDRWFHLDPPRHLTFFTKAMLSEMFA
                     ARGLVDIEVKDLPIPTGLAGSLSYKVWGKFEPVTWYSLIVPGLVFSKLVRDGNFAISA
                     RRPL"
     gene            complement(12029..13033)
                     /gene="gmd"
                     /locus_tag="VFV99_03885"
     CDS             complement(12029..13033)
                     /gene="gmd"
                     /locus_tag="VFV99_03885"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5058472.1"
                     /translation="MKKALVTGVTGQDGSYLAELLLDRGYEVYGVVRRSSSFNTERLD
                     GIYQDPHVSDYKLRLVYGDLEDGSGLANLIKRIQPDEVYNLGAQSHVRVSFDMPEYTA
                     STVAMGTLRLLEAVRELDRKCRFYQASSSEMYGSAPAPQSERTPFQPRSPYACAKVFA
                     HQLCQNYRDAYGMFICCGILFNHESERRGIPFVTRKITRAAARIKHGLDKKLFLGNLD
                     AKRDWGHAKDYVEAMWLMLQQDTPDDYVVATGESHTVRECLDVAFGALDLDWHQYVEL
                     DPRYLRPTEVDHLLGDATKAREKLGWKPRITFQQLITQMVHADEQDVRQSLTGRAPRM
                     "
     gene            complement(13030..13986)
                     /locus_tag="VFV99_03890"
     CDS             complement(13030..13986)
                     /locus_tag="VFV99_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX5058473.1"
                     /translation="MLDSPLIGKRIVVTGGAGFLGSFVVDTLRARGVKDIIVPRKQQY
                     DLVEREACRRLLADARPDVVFHLAARVGGIGANRANPGLFLYENAMMGLQLIEECRLA
                     NVGKVVIAGTICAYPKFAPVPFKEDDLWNGYPEETNAPYGIAKKLLLVQSQAYREQYG
                     LNSIMLFPVNLYGPRDNFDLASSHVIPAMIRKFLEAKERNATEVPLWGDGSPTREFIY
                     VEDAAEGLVLAAERYDSSDPVNIGTGNEISIRDLATQIAAACGYTGRFVWDPSQPNGQ
                     PRRRLDVTRAKERFGFVAKTPLAVGLERTVAWAIQQRKAGQL"
BASE COUNT         2404 a         4584 c         4582 g         2499 t
ORIGIN      
        1 acatcgcttg ccttgtatca cgtgtccgtc ggtttcgacg tgcggtccac ctcagcgcgc
       61 agaatggcga gctcgcgagt caagtccgtg atctgctgct gcatttcgac gaacttcgtg
      121 tagtagtgca tgagcacgaa caagacgccg gtgatgctga tgtagaagat gaagtcagcg
      181 ccgcggccga caccaaccat gtgagctacg tcgtcggcga ggttcggatc gatgaccgca
      241 gcagcgcctg ccgccagcag cccgaacacc gtcatgatgt gcagcggcag ccggttgcgg
      301 cgcagaaaga ttagatagcc ggtcgcggcg atcccgagca ggagcaggat gcggatgatc
      361 atgcgcggaa gaagtagtcg aagagaatgc gcaccgcttg gatgccaccc tggcctttgc
      421 tcatcgagtg ctcggtgtag cgaatcgtca ccgggacctc gacgacgttg agcttgctcg
      481 tccggatctt gcggagaagc tccgatgcat gcgccatgcg atcctgagtg atgcgaagct
      541 tttctgcgat cgacgcgcga aacgcgcgca agccgcagtg cgagtcggtg agcttcatcc
      601 cggagaggct gttcgacacg acggttgcgg tcttgagcat cgccatgcgg ctactactcg
      661 caccttcaac cttgccgagg aaccgcgaac cgagcgcgat gtctgcctcg ccgagtgcgt
      721 cgatcagcgt cgggatgtcg ccggcgtcgt gttgcccatc ggcgtcgaac gtgacgatgt
      781 gcttcgcgcc gcggcggagc gcgtagtcga tcccggtctg cagcgccgca ccctgcccgc
      841 ggttgatcgc gtggcgcacc acgatcgcgc cgggtacccg cgcagcagtc gccgtgtcat
      901 cgctcgagcc gtcgtccacg acgacgaccg gccagccatc gcggaccaca ctggacacga
      961 catcgcggat caccttgcct tcgttgaagg cagcgatcac gacccatgtc ccgcgccgat
     1021 cctgctcggt caccgaccct tttaaacatg gtctccgacg aggagcaagt gttgatgatc
     1081 acgcgagaac cgttacgtga cagaagcggg agaagcgggc acgctcggaa cgtgcctcgc
     1141 tggctgattc tcgtatttct cgcagcctgt gacgggccgg tgaacgagat ctgggtcgcg
     1201 cccgatggca atgacgccgg ccccgggaca caggaccaac cgtttgcgac gctcgcccgc
     1261 gcacgaaatg cggtccgcga gctcggtccc gatcatccgc ggatgaccgt gtacctccgc
     1321 ggcggccggt acgttctcga ggatgtgctc gtgctcgacg agcgtgacgt caacatcgac
     1381 ttcgtagcgg cgccgagtga gccacgcttc gtgacggcgc cgagagagac gcccatcgtg
     1441 agcggcggca ggatcgtcaa cggctggcgt gccgacggcg atcgcatgat cgcgagcacg
     1501 ggcgtcgatg cgtttcggca ggtctgggtc aacggcctgc gccagcctcg cgcgcgcggc
     1561 ggcctaccgc cgctcggatt cagaggtgac gtcgatagca tcgccggcag cgccggttac
     1621 ttcgccgggg cgttccccgc aatcggggaa ccgaaccagc tcgatttcgg ctacgagctg
     1681 acctggcagc acaagatctg cccggtgcag tcgatcttcc acgcgatcaa cggaggcgtg
     1741 aacatcgaca tgcagcagcc gtgcttcttt ctgatcacgc accacgtccg gcctgctcaa
     1801 caaccggcgt acatcgagaa cgcagccgag ctcatcgacc agcccggcga gtggtacggc
     1861 gcgcacggcc cccggcggta tctacctcga cgagggctcg ggatttatcg aggtcgtcgg
     1921 aaactcggtg ttcgacgtcg tcgagccgta cttcgcaaac aatctcgcgc agggccgcga
     1981 cgcgacctgc aacgtccacg acaacttctt cggagtcgat gtcgacgtcg aggccggact
     2041 gcttcccgaa taccgcgatc tgctcgacga ctaaccgcgc tgcgtgatac gtggaggagt
     2101 ccatgccccg cgtcgcgatc gccatgcccg tctacaacga cgagcagtat ctagcgctcg
     2161 cgctcgaggg tttgtgccgt cagacggatc gcgacttcgc actcacggtc gtcgacgatg
     2221 gctcgacgga tgcgtcggcg gccgtcgcag agtcgttcgc cgatcggctg ccgatcaccg
     2281 tgatccgcgc cgagcaccgc ggcttgcgga cgacgaaacg cgcagcggtc gaagcatcat
     2341 cgcccgatgc cgaattcgtc ctcatgcacg atgccgacat cgagttgccg gacgacgcga
     2401 tcgaacgcat ggtcgccatg ctcgactccg acgcgaatgt cgctgccgtc agcgcgaccg
     2461 cgcgctcggt accgagtcgc cggtggggac gaggacaggc gttcttcgag tatctcgtcc
     2521 gccattcact ggtgaacgat cgggacgaga gccgcggaat tgtcggcggc tgtgtaatgc
     2581 tccggcgcac tgcgctcgct gggattcgtc tccgcgtcga catcaacgag gatgtcgatc
     2641 tgtcgctgca gctgcgcaac cgcttcaagc tgatgtggcc gcgcgacctc gtcgccgatc
     2701 actggggtgt gccgactacg ttgcgcggaa tctggaaccg cggctatcgc gatggcctcc
     2761 gcgtgcgcgc gatcatgcgc gtacacccga aagactttca gctcggcacc gtcgcgcgcc
     2821 tcgtgccgct gccgctgagc gcagcgatgg cgatcggcgc gctcacgttg caaccctggt
     2881 tagtcggtgg cgccgccacc gtgctggctg cgtacacggc cgcgttcttg tatgcctcgc
     2941 gccacgtccc gggtgatctc gggacgcgac tcgagggaac ggccttgttc ctgctgagca
     3001 acttcgggtt tgcagccggc ttcgtcagag aatcgctgtc gaagcccacg gacgaagagc
     3061 tgagcgagcc gccgcggacc gggtaacaga ccgactacac gccgcgcagc gatccgcgaa
     3121 tcgccgtgat tcgcgccgcg agcttcttcg catgcggctg gccgctcgcg aggaaacggt
     3181 cgatgtaatc gcgcgcactg ccggcgcggc ggagaccctc gagcaggacc aagtactcct
     3241 cgccctcgag accacgctcg gagcgataga tcacgaatgg aaacgtcgcg gtttcgccgc
     3301 gcgccttctt caaattaacg gtcggcgacg ccttctcgac ggccgacagc acgtcggagt
     3361 tgatcgcgca cagccacagc gcgagctcgg tcgcgtcgaa cgagctcacg tgctgctcga
     3421 gctgctgcag ccggctgacg tagccgagca tgcggaactc tttcatccag gcagtgaacg
     3481 agtgcactgg atatgagtgg tacgcctcgg ctgacagctc ttcgtgcggg cggtactcga
     3541 cggcgagatc cgagaggtgc gcgcgctcga gtggcggctc ggtcgcgagc catgcgcgct
     3601 cacgtgcaac accgtcgggg atcaggaacg ggctctcgcc gtcgcgccac gagccaagca
     3661 gcgggacgcc attgtcgcgg agcacgttcg cccatggcgc tgcatcgagc tcgtggatcg
     3721 aatcgagata tgcctgcgtg agcgcgccgc gtaccgggag acagcgcagc tgcgcgccag
     3781 cgatcccttt caacacgtac ggctcgcgcg gaatgcgctg ctgcagctcg gccgagaagc
     3841 gcgctgcgtt cacggtgacc gcggagaagc cgcgctcgac gagcagcgac acttgctcgt
     3901 tcgtcagcat gaactccggc ggcagataca gtggatgcac gcggttgaat tcgcgttgca
     3961 ggtagcgcca tccgagatcg aggttgatca gaaagccctc gcggctgcgg agcagcgaga
     4021 tgtcgtgcga gaacggccgc gcgatcgact cgaagatgtc gggatgcgcg ttgacgaggt
     4081 cgatcgcttc gggattgaac gcgcgcatgt cggcgacggt cgccccggtg aagccgagca
     4141 tgaccttctt gcgtagctcg acgtagaggc gcaaccagct gcacaccgcc gactgctcgc
     4201 cgcctgcgag cgcctgcagc caggcaccgg agctcaggca cgacgcatcg gcattgaagt
     4261 tgacgatgtc gacgatcgtg gtcatggcag cagcgcggat tcgaagtcgg ccgacgtcgc
     4321 gatgaactcg gcgagcttcg cgtcgtcgat cgggatgccg cgcgccgtgc gctcggcgaa
     4381 cgcgcgcttc tccggatcgc ccggcaccat caccggcgcc gccggatcga gtggctcgag
     4441 cccacgcgcg cgctcgacca tgtccttcat gcggtgcgcg aagtcgccga gcgcagcaaa
     4501 ccgcgcgaca tcgatcgcca tgaagcaatg gctgatcgcg cgccgcacct cgacgtgctt
     4561 gtacatcggc aacaggtcct tgctgatgcc gccgccggcg agcagcgcgc acaggatgtc
     4621 gacgacgatc gacaggccat agcccttgta caggccggcc ggttgcagcg agcgcgccgc
     4681 attcgcgtcg gtgaccggca ggccgttctc gtcgcacgcc cagtcctcgc cgagcgagcc
     4741 accggtgcgc cgcgcgttga cgaccttgtt ccacgacacg agcgaggttg ccgcgtcgaa
     4801 gcagaacggc tgctcaccct cgatcggcgc agtgaagcag atcgggttcg tgcccgtgaa
     4861 cgagtgcttc gcgcctgtgg ctttgaccag cgcgtcggcg ttcgtgaacg cgaggccgat
     4921 gcagcccgcg gcgggtgcgc gtaacgcgaa gtaggcagcc gcaccgaagt ggctcgagtt
     4981 gcgcacggcg acgaccgcaa tgccgacttg cttcgcgcac tggatcgcgt gatccatggc
     5041 gaccgcaccc gcgtggtggc cgtatgcgtg atcggcgtcg aggatcgcgg tgcccggggc
     5101 cgtctgctcg aacgcgagct tcggcgcgag gttgatgcgg ccggtctccg cgacgcgcgc
     5161 gtagtgcggg aacagctgga tgccgtgcga gtcgacgccg cgcagcgacg tctggatcac
     5221 cgacgccacg acatgcgcga cggcttccgg cgcgacaccg cgggcaccga gcacgcgagt
     5281 catggtcgcg cgcagcttct cggcatcaaa gcgacgcatc gatcgtcctc cattccgaac
     5341 cgtgcgcgag gctctcgttg accgcctcga cgatcgcgac cgataccagc gactggcgca
     5401 acgttggccg cagtggctcg agcttggcgc gcgtcgtctt gcccgcctgc agtgcgagca
     5461 cgtcggcaaa gaacgctgcg atgctcttgt agccgtagcc ctggaagtcg agcgagccgc
     5521 tgtcgctcgg taggtagtcg gcgaagtatg ggttcacgtg ctggattccg tcgcggttgg
     5581 tgacgacctc gatgccgcga tccttctgat cgagctcgat gcgaccttcg ccgcagatga
     5641 ccttcatctt ttgatctgac agggcgcttg tcgtattcgg atcgatccag ttgatcgaca
     5701 gcaggctcac gaagcgatgc tccgcgtcgc cgggcttgcc ccacaggacc tgcgcgtgga
     5761 ccgagtcgta cgtgttgacg ccgcgcgacg tcagcacacc gcgcgtaccg accgccatcg
     5821 cgcgcagcgg cgtgaggccg gtcaagaagt acaacaggtc gacgtagtgg acgccgaggt
     5881 actggaagat gttcgtgcga tcggcccagc ccttgaacac ctcggtcgga atgcgaatgc
     5941 gctggctgta gtcgacaacc gcgtagacga gcgggccgag gcgattctcg gcgatgtagc
     6001 gccgcgtcat caaattactc tcgtcccagc gcttgtgaaa ctcgacggcc gcatacgtgt
     6061 cggtgcggtc ctggatctcg atgagctttc gcgcctcggc gaccgtcggc acgagtggct
     6121 tcacgacaag cggcggcacc cgcgcttcga gcagctccgc cgtgtagcgg aagtgcaagt
     6181 gatcgggcac gacgacgatg ctgcaggcga agtcggcgag cgatagctgc tgatcgagcc
     6241 ggcgatcgcc gtcgatcacc tggtagcgga acgcgagcga gctaccgagc agctggttca
     6301 cgcgcgcggc cgcctcggtc acgagtgccg cattgcccgg gtcgcgcgcg acgacgagca
     6361 cctcgtcgat cggcatggtg cgcgaggcct gcgcgagcgc agccatcacg gttcccgcgc
     6421 ccgtgccgtg ccgtcccgtc acgtacatgc cggcgccgac gacgagtacc ttcacgagcg
     6481 acctccgcct tcgagcacga aacgagcaaa cgcgaggtcg cgtggcgtgt cgatgtcgag
     6541 cacgtcgttg gcattcatga cgtaggcgag catgcggtct ccggtcatcg actgcttgtc
     6601 gaggatcgtc gagcgacgag tcacatcgat cacgcagttg tagtagtaga gcggcggcag
     6661 atcctggcgg cgcagcaggt agggctgcgg atgctcggcc ttcatcagcg gatccagaac
     6721 gccatcgtca ccgatcttga acacgcgcca cggatgctgg tcgaccttcg acaccgagcg
     6781 tacggagtcc gcgttggggt tcgctcgcaa cagctcgacc gcttcgtcga tccagcctgc
     6841 cttgcgatac ggcgcggttg gccgcagatg cacgacgacc tcgggcttgt cgccatgcgc
     6901 ctcgagccag cgcagcgcgt gctcgaacac aggccagtcg agcacgagat cgcccgcaag
     6961 ctcgtcggga cgcaggaacg gaacctcggc cccggccgca cgcgcagcct ctgcgatctc
     7021 ggcgtcctcc gtcgacacga tcacgcgatc gacgagcttc gcgtccaacg cgtgctgcac
     7081 cgagtgcgca acgagtggct tgccggcgag tggcaccagg ttcttgcgct tgatgccttt
     7141 cgagccaccg cgggcgggaa tgatcgcgac gacgttactc atgtgcgtgc ccacaacccg
     7201 gtgcgcttga cgatcgcacc aaccgcgttc gtggcgcgcc ggcgcagatc gccgaggccg
     7261 ccgttgcgga ggcgcagcgt cttcgcgtgg cccttcgtga tcccggcgcg agcgaggcgc
     7321 gcctcgtggc tcagtagctc cgtcagatac ggcgcgaaga gagcggtgcg cagcgtgtcg
     7381 tcgagcctca ggccgaacgc acgcagaccg gtctcgtaga gcggtcccac cacgtgcttg
     7441 aagccgtgat agtggaagca gatcaatggt tggtcgtcga tcatcgtgcg gcctgcgtcg
     7501 atgcgcagtt tctcgggctc gagattccac ggcgcgacgc tgacgccttt gtgctgcagg
     7561 atcgccagct gcgagaacag acgcggccac tgctccaggt atttctgatc ggcaaacttg
     7621 tcgccctcga tgcggtcgta gcaccactcg atgcagcgct cgcgccacca cgccgcgcac
     7681 gccttgccct gctcgtcgtt gcgaaagctc agccagccga cgttgaaacg cccccacttc
     7741 tccagatgtg cgcgcgccgg cgggaaacga tgctcgacga cgccgaccga cttgtctccg
     7801 agctcggtaa acagcggctc gggcgacgag aagaagcgga agtcggcatc gacatacgtg
     7861 acgatatcga ggtcgtaggt cgcgagcacg tagcacaccc acgcgggcgt gatcgtgaag
     7921 tagtactcga caagcgagcg cgtcggccgc gtcgcagcga gcgccggatc tgcagcctcg
     7981 agctcggagc gcttcacgag cacgacctcg ggaagtgcac gctcgctgag gtagcgatgc
     8041 gcctcgtcgt cgaggcacag cacgtaccag cggaagtcac ccgcgtgcgc gcgcagcgac
     8101 tcgtagagtg cgaggccgcg cgacagatag ttcttgtcga agaacgtgca gaagtggcgc
     8161 gtcgccatca cgtcaccttc cagagcccgg tgcgcaccgc gatgccgtac gtgtagatca
     8221 cggcctcgcc cattgcgagc ttgacccagt ccttggcatc gagcccgagc ttcggaatgt
     8281 agcggcgctt gatgcgatgc atctcgcgaa tctgcttcca gacgctgtgc acgctcttcg
     8341 catcctggtg ggacacgatg cacgcgagat cctcgtcgag gaatcggcgc gagcgggcgg
     8401 cgggaaacag ccgcatccac aggtcatagt ccatcgccag gtgcaggccc tcggccacac
     8461 cgccgacgtc gagaaagagc tggcgcttga agaacaccgc cggctgcgcc acgaagcagc
     8521 gaccgtggat cgccatctct ttgagcgcgt tgatttgata cgcctcgaac cgcttgagcg
     8581 gcttgtgctc gatgtcgacc atcatgccgt ggccgtagac gatgtcgacg tccggtgcct
     8641 cgtcgaactt cgcgagcacc cggcgcagca cgtcaccgtt cgggtagtaa tcgtcggagt
     8701 taatccacgc gatgatctcg ccgcgcgctg ccttgatgcc cttgttgatc gcgtcggact
     8761 ggccggagtc cttctcggac acgaaccgca gcttgtcgcc gtagctcttc aggacctcga
     8821 ccgtgttgtc ggtcgacgcg ccgtccatca ccacgatctc gtagctcgga agctgctgtg
     8881 cgaggcagct gtcgatgcac gtgcggatga actgcgcgtg gttgcgcgtc gggatcatga
     8941 acgagacgcg cggatcagcc acggaagaac tccgcgatgc gcgcacagac cgtgtcgatc
     9001 tcgtcgtcgg tcagctcggg gaagatcggc agcgacagat actcctgcgc gcactgctcg
     9061 gcgaccggcc agctaccggc gagcgcgagt gcgggctgct tcggcaagat gatcggatag
     9121 tggacgtgcg tcgtgatgcc gtgctcgcgc aggaactgac cgagcgcatc gcgcttcggc
     9181 gtgcgcacga cgaacaggtg ccacaccgac tccccgtgct tctcgacggg aagctgcacc
     9241 ggaagatccg caagggttgc acggtaccga gccgagatcg cgcgccgccg agcattgtgc
     9301 gcaggcagat ggcgcagctt cacgcgcagc agcgctgcgt gcagctcgtc gagccgactg
     9361 ttgaggccga ggtcagcgtg ctcgtagcgg ttcgtttggc cgtagttgcg caagcggcgc
     9421 acgcgctcgg cgagcttggc gtcattcgtg acgaccatgc cgccgtcgcc gaacgcaccc
     9481 aggttcttcg tcgggtagaa gctgtagcaa gtggccgcac cgaacgtcgc gacgtcgcgg
     9541 ccatcgtgtg tcgcgccgtg cgcttgcgcc gcgtcgccga ttagcgtgag cttgtgccgc
     9601 gtggcgatcg tctcgagcgc ggtgaggtcg gccgcttgcc cgtagatgtg caccggcaac
     9661 agcgcgcgcg tgcgcggcgt gatcaacgct tcgacccgcg ctggatcgat cgagtaggtc
     9721 tgcggatcga tatcggcgaa taccggtgtg gcgcccgcac gcacgatcgc gagcgcggtc
     9781 gcgaacgccg agttcgtcgt cgtgatcacc tcgtcgcccg cgccgatatc acatgcacgc
     9841 agcgcgagct cgatcgcatc catgccgttg ccgacgccga tggcatgcga aacgcctgcg
     9901 aacgccgcca tctcgcgctc gaacgcctcg acttccttgc cgaggatgaa ccagccgctg
     9961 tcgagcacgc gcgcgaacgc ctcgtcgagc tcgcggcgca tcgtcgcgac ctggcgcacg
    10021 aagtcgttca ttggaatcgt cacggatatg cctcgtgcgt ccagtagtgc tcgcgatgct
    10081 cgcggtagta ctcgaccgtg tcggccatgc cgcgcgcgag atcgatcgcc ggctcccagc
    10141 cgagcacgcg gtgggccttc gtgtagtcgc cgacgtagtc accgatctcg atgctcttca
    10201 ggtgtggcgg atacgggacg ttcgtcacgc tgcccgactt ggcctgcgcg acgatcgtct
    10261 gtgcgatctc gcggaggctg atcggcttct tcgaaccgag attgaagacc tcgccatcgg
    10321 ccgctggaga caccatcgcg atcaatagcg cggcgacgac atcggacacg tgattgcagt
    10381 cacgcaactg ctcgccgccg tcgaacaacc ggatcgcctg gccgtcgagc gcctggcgca
    10441 cgaaccaggc cagcacgccc tgacgcgcgg tgcgcatcgt gtgccgtggc ccatacgtgt
    10501 tggtcagccg cagcgaggtc acccgaagcc cgtatgcgcg tgcgtacacg aagtggtagg
    10561 tctcgcctgc gtgcttgttg atgccattga cgtcgatcgg cgcgatgtgc gcgctctcgt
    10621 cgacaaggcc ggcggtgcgc ccgtactggc cacgcgtccc cgcgaacacg accttcacat
    10681 cgggccgctc tttgcgcagc gcttcgagca ggccgatgtg cgtcatcaca ttcgtgcgca
    10741 ggtcggtcag cggatcttcc atgctgtcga cgtggctgac ctgcccgacc atgttgaaca
    10801 cgctgtcgaa atcgcgcacg agcttggcca tctcgcgctg gtcggtctga tcgccctcga
    10861 tgaaacgcag ctcgacgccg ccgaggttga accggtttcc gccgtagttc ggcgcgaggt
    10921 tgtcgagcac ggtcaccttc gcgccgagag ctacgagcgt gcgcgccagg ttcgatccga
    10981 tgaagcccgc accgccggtg acgaggacgg accggccgct gtaatacgcg cgcacgtcgt
    11041 ctcgccggct gacgagctcg gggatcgcgg gtagctcctg gaccgtcatc gtcgagcgac
    11101 gttcttatca gggcgctccc gccctggaaa gggaccccgc cgagactaag tcaggcgcgg
    11161 agttccggta tggtggggcc gcttgatgtt cgaatgccgc aactgcaagg cgcaccgcgc
    11221 cgagcccatc tatcatcagt tgcgcgatcg cttcgaaggg caccccggca cgttcgatta
    11281 cgtccgctgc ggcgagtgca gcctggtcca gctcgcggag attccgcctg atctcgaggc
    11341 gctctacatg ggctaccgcg tgcacggtcg aacgtcgtcg ctgtatcacc tcctccgcaa
    11401 gctcacgatc ggtcatgcct acctctccac atccggtgag gggcgctctc tgctcgactt
    11461 cggctgcggc agtggctggt acttgcaaca gatggccgag ctcggctggc aggcaaccgg
    11521 ctacgagtca aacgcagatc atgcggccca cgtgtcacgc gtcacgtcac tgccggtgct
    11581 gtctggtctc gattcgctga aggcgcgtac cggcgagttc gatctggtga cgatgaactt
    11641 cgtgttcgaa cacctcgccg acccgcacga gatgctcgat ctcgtcagcc gtgtgctcag
    11701 acccggcggc cagctctaca tctcggtgcc gaacatcgag agccgcgagg cccgcatctt
    11761 caaggatcgc tggttccacc tcgatccgcc tcgccacctc acgttcttca cgaaggcgat
    11821 gctgagcgag atgtttgccg cgcgcggcct cgtcgacatc gaggtcaagg atcttccgat
    11881 tccgaccggg ctcgcgggca gcctctcgta caaggtctgg ggcaagttcg agcctgtgac
    11941 ctggtactcg ctgatcgtgc ccggcctcgt attcagcaag ctcgtgcgcg acggcaactt
    12001 cgcgatctcg gcacgccggc ccctgtagct acatgcgagg cgcgcggccg gtgaggcttt
    12061 gacgcacgtc ctgctcgtcg gcgtgcacca tctgcgtgat cagttgctgg aacgtgatgc
    12121 gcggcttcca gccgagcttc tcgcgtgcct tcgttgcatc cccgagcagg tgatcgacct
    12181 cggtcggccg caggtagcgc ggatcgagct cgacgtactg atgccaatcg aggtcgagcg
    12241 cgccgaacgc gacgtcgagg cactcgcgca cggtgtgtga ctcgccggtc gccaccacgt
    12301 agtcgtcggg cgtgtcctgc tggagcatga gccacatcgc ctcgacatag tccttcgcgt
    12361 gtccccagtc gcgcttcgcg tcgaggttgc caaggaacag cttcttgtcg aggccgtgtt
    12421 tgatgcgtgc agccgcgcgc gtgatcttgc gcgtcacgaa tggaatgccg cggcgctcgc
    12481 tctcgtggtt gaacaggatg ccgcagcaaa tgaacatgcc gtacgcgtcg cggtagttct
    12541 ggcagagctg atgcgcgaac accttcgcgc acgcgtacgg gctacgcggt tggaatggcg
    12601 tgcgctcgct ctgcggtgct ggtgccgagc cgtacatctc gctcgacgac gcttgataga
    12661 accggcattt gcgatcgagc tcgcgtacgg cctcgagcaa gcgcagcgtg cccatcgcga
    12721 ccgtcgacgc cgtgtactcg ggcatgtcga agctgacgcg gacgtggctc tgcgcgccga
    12781 ggttgtagac ctcgtcgggc tggatgcgct tgatgaggtt cgcgagcccg ctgccgtctt
    12841 cgagatcccc gtagacgagg cgcagcttgt agtcgctgac gtgcggatct tgatagatac
    12901 cgtcgagccg ctcggtgttg aacgagctcg agcgacgaac cacgccgtag acctcgtagc
    12961 ccctgtcgag cagcagctcg gctagatacg agccatcttg tccggtgact cccgtaacga
    13021 gcgctttctt cacagctgac ctgctttgcg ttgttgaatg gcccacgcga ccgtccgttc
    13081 gagtccgacg gcgagcggcg tctttgcgac gaacccaaac cgctctttcg cacgcgtgac
    13141 gtcgagccgc ctgcgcggct gaccgttcgg ttgcgacgga tcccagacga agcggccggt
    13201 gtaaccgcac gccgccgcga tctgcgtcgc gagatcgcga atcgagattt cgttgccggt
    13261 gccgatgttg accggatcgc tcgagtcgta gcgctcggct gccagcacga gtccttccgc
    13321 cgcatcctcg acgtagatga actcgcgagt cggtgagccg tcaccccaca gcggcacttc
    13381 ggtggcgttc cgctccttcg cctcgaggaa cttgcgaatc atcgccggga tcacgtgcga
    13441 gctcgcgaga tcgaagttgt cacgaggtcc gtagaggttc accgggaaca gcatgatgct
    13501 gttgagcccg tactgctcgc gatacgcctg cgactgcacg agcaggagct tcttcgcgat
    13561 gccgtacggt gcgtttgtct cctcggggta gccgttccag aggtcgtcct ccttgaacgg
    13621 caccggcgcg aacttcggat acgcgcagat tgttcccgcg atcacgacct tgccgacgtt
    13681 cgcgagccgg cactcctcga tgagctgtag gcccatcatc gcgttctcgt agaggaacag
    13741 tcccggattc gcgcggttcg cgccgatccc accgacacgc gcggcgagat ggaacacgac
    13801 atcgggacgt gcgtcggcaa gcaagcgccg gcacgcctcg cgctcgacga gatcgtactg
    13861 ctgcttgcgc ggcacgataa tgtccttcac gccgcgggcg cgcagcgtgt cgaccacgaa
    13921 gctgcccaga aagccggcgc cgccggtgac gacgatccgc ttgccgatga gaggagaatc
    13981 aagcacgcac gagactctta ctataggatt cgcgccgcac gccatgcctc gcctcgccga
    14041 gctcattccg cccgtcatgc atcgctggc
//
LOCUS       DASUFX010000100         4616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1962304, whole genome shotgun sequence.
ACCESSION   DASUFX010000100 DASUFX010000000
VERSION     DASUFX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962304"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFV99_03895"
     CDS             <1..413
                     /locus_tag="VFV99_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058474.1"
                     /translation="GLGKWLPLGVIALLLMISGPSMLLAWLKLRRRNLGPILDANGWA
                     INSRARINVAFGAAMTELAAIPAGSRRSLSDPFADRTPPWRLYIALTGLLVLGGTWYV
                     GRLDRYLPNSIRSTTVLGEYAPAYKPPAETPPAK"
     gene            454..1704
                     /locus_tag="VFV99_03900"
     CDS             454..1704
                     /locus_tag="VFV99_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX5058475.1"
                     /translation="MRFTPRRYAKPRTVDELAALVRDSKRIRAVGAAHSWSPAIVTDD
                     TLVSLDRMRGVIALDRDAKRITVQGGMRLRELNAYLDREGLALASLGSIDSQSVAGVI
                     ATGTHGSGKNFRCLSAQVAKLDLIDGTGNRVTLERGQPDFDGAVVGLGALGIVHAVTF
                     DVVDAFRLHDRTGLAKLDEVIEAIDEHVASADHFKLWWFPPHDHAIIYRYMRTDRASN
                     DARFRRWFKERVIAVGVYRSLVAIGQLSGRRWIPAFNRFLTGQAGQPLNRITPSHIGY
                     LTPIPPVHRESEWAFDIKDAKPLLREYRRLLPGDGHTYNFIQELRFSRADNLWLSPGY
                     QRDSIWLSLYNIDRKNWSAQLAKFEAFARANGGRPHWGKEATFDRTYLRGQYARLDEF
                     AALAARFDPDRKFRNAWLDEILGV"
     gene            1982..2518
                     /locus_tag="VFV99_03905"
     CDS             1982..2518
                     /locus_tag="VFV99_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058476.1"
                     /translation="MSEPATVTEKLVGSHREFLEYVQRRVGDRALAEEIVQDALVRSL
                     EHEGDIRESAIGWFYRVLRNAIIDRARRAAVQRGRLDALAHEPEAAPDAELQAVVCRC
                     VATLADTLKPEYADALRRIDIEDMPVKDYAEQAGISASNAGVRVFRAREALRKQVARA
                     CGTCATHGCLDCSCGTTS"
     gene            complement(2508..4028)
                     /locus_tag="VFV99_03910"
     CDS             complement(2508..4028)
                     /locus_tag="VFV99_03910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058477.1"
                     /translation="MGRRDVWTPPVEWSGQGRASVRRGLRAETFGDEKSTIILSGEIT
                     APRAWPRNDSPDPTATFGEYSIGEQLGTGGMASVHRAERRVKGVRQRVALKRMLPHAA
                     ADPDLVKLFLDEARLVSLLRHRNIAEVYDFGTVGEEYFLAMELVAGPTLKQLLRHGAA
                     TVGLIPYPIALNLLIQICDALAYAHDRCDEHGKPLRIVHRDVSPSNIVISSNGVVKLI
                     DFGIAKTVSTHTQAGIIKGKLGYIAPEYLEDTGLDRRADLWAVGVVAYEMLCNARLFD
                     ADDDFEMMRKVRSEPITPPSTYNHDIPAELESIVMTALQRDPMLRWQNATALRNALAG
                     VAKPSSNAEVMEWVEWVFKLAGETARPVRGSRPITAQPAPRTSAASLVDAKPILPPPL
                     VPSMPTVQLAPVLQTPVPMQRPQTPAPLPLPPEPTPPPVVYQPMRALQLRAPPPLQLR
                     APRPLQPRWPAVGAAMVMRRRRRRIILPLFLAMLGAFAGALSAPLVYELLAQYFNW"
     gene            complement(4174..>4616)
                     /locus_tag="VFV99_03915"
     CDS             complement(4174..>4616)
                     /locus_tag="VFV99_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058478.1"
                     /translation="CLQRRIGSVMAGAVRLELAFVSNNSETVTRMLLDFQLEDVVRGR
                     RGAHADDVALGYRKLTMSSDPSVRAAAHARLAQLARVTKHSANAATETEACIREVRSS
                     LVADGWGRICERERAALKLPPLDPLRERLAAPTASTDIAIEPPL"
BASE COUNT          779 a         1538 c         1559 g          740 t
ORIGIN      
        1 tcggcctcgg caaatggttg ccgctcggcg tcatcgcgct gttgctcatg atctcgggcc
       61 cgtcgatgtt gctcgcgtgg ctcaagctgc gccgccgcaa cctcggcccg atcctcgacg
      121 cgaacggctg ggccatcaac agtcgcgctc gcatcaacgt cgcattcggc gctgcgatga
      181 ccgagctggc cgcgatcccc gcgggctcgc gccggtcgct gtccgatccg ttcgctgatc
      241 gcacgccacc gtggcggctg tacatcgcgt tgaccggtct gctcgtgctc ggcggcacct
      301 ggtacgtcgg ccggctggac cgctacctgc cgaattcgat ccgcagcacg accgtgctcg
      361 gcgagtacgc acccgcgtac aaaccgcccg cagagacgcc gcccgcgaag tagccgcgtt
      421 actctggcgc agatgcgcaa ctacggtggg aacgtgcggt ttacgccgcg acgctacgcc
      481 aagccgcgca ccgtcgacga gctcgcggcg ctcgtccgtg acagcaagcg cattcgcgcc
      541 gtcggagccg cgcattcgtg gtcgccggcg atcgtcaccg acgacacact ggtctcgctc
      601 gatcgcatgc gcggggtgat cgcgctcgat cgcgacgcaa agcgcatcac cgtgcagggc
      661 ggcatgcggc tgcgcgagct caacgcgtat ctcgatcggg aaggcctcgc cctcgcgagc
      721 ctcggctcga tcgacagcca atcggtcgcc ggtgtgatcg cgacgggcac gcacggtagc
      781 ggcaagaact ttcgctgcct cagcgcgcag gtcgcaaagc tcgatctgat cgacggcaca
      841 ggcaaccggg tcacactcga gcgcggccag ccagacttcg acggcgccgt cgtcggcctc
      901 ggcgcgctcg gcatcgtcca tgcggtgaca ttcgacgtcg tcgatgcgtt ccggctacac
      961 gatcgcaccg ggctcgcgaa gctcgacgag gtaatcgagg cgatcgacga gcatgttgcg
     1021 agcgccgacc acttcaagct gtggtggttc ccgccgcacg accacgcgat catctaccgc
     1081 tacatgcgca ccgaccgcgc gtcgaacgac gcccggttcc gccgctggtt caaggagcgc
     1141 gtgatcgcgg tcggcgtgta tcgcagcctc gttgcgattg gccagctgag cggccggcgc
     1201 tggattcccg cgttcaaccg cttcctcacc ggccaagcgg gccagccgct gaatcgcatc
     1261 acgccgagcc acatcggcta cctgacgccg atcccaccgg tgcaccgcga gtcggagtgg
     1321 gcgttcgaca tcaaggatgc caagccgctg ctgcgcgagt atcgccgcct gctgcccggc
     1381 gacggccaca cgtacaactt catccaggag ctgcggttca gcagagccga caatctttgg
     1441 ctcagccctg ggtatcaacg cgactcgatc tggttgtcgc tctacaacat cgatcgcaag
     1501 aactggtcag cacagctcgc caagttcgaa gcgttcgcgc gcgcgaatgg cggccgcccg
     1561 cactggggca aggaagcgac gttcgatcgc acctacctgc gcgggcagta cgcgcgcctc
     1621 gacgagttcg cggcgctcgc cgctcgcttc gatcccgatc gcaagttccg caacgcgtgg
     1681 ctcgacgaga tactcggggt gtaatgccgg cgcccatggc gcgtcgtagg ggcaaacgcg
     1741 caatcaagcg ccaggccccc ggagacaacc atggaaacga agaacgagac ctcgaactgt
     1801 cgctgtgcca acgccacttg cccctgcgct cagccgcaaa gccagcggtg cacctgcggc
     1861 gatcagtgca agtgtgcggc gacctgccgc tgtgaccagg agtgccgctg cgcagctgca
     1921 aaatagtgtg tcgccgtgtg taatgccggc gcggcgaatg cgtcgtacgg tcatggagac
     1981 catgtccgag cccgcaaccg tcaccgagaa gctcgtaggg agtcaccgcg agtttctgga
     2041 gtacgtgcaa cgccgcgtcg gcgatcgtgc cctcgccgag gagatcgtcc aggacgcgct
     2101 cgtgcgcagc ctcgagcacg agggcgacat ccgcgagtcg gcgattggct ggttctatcg
     2161 tgtgctgcgc aacgcgatca tcgatcgcgc gcggcgcgcg gccgtgcaac gcggacgcct
     2221 cgacgcgctc gcgcacgagc ccgaggctgc gcccgatgcc gagctgcaag cggtcgtgtg
     2281 tcggtgcgtc gcgacgctcg ccgatacgct caagccggag tacgccgacg cgctgcgcag
     2341 gatcgatatc gaagacatgc cggtgaagga ctatgcggag caggccggca tctctgcgag
     2401 caacgcaggc gttcgcgtgt tccgcgcccg cgaggcgctg cgcaagcaag tggcgcgcgc
     2461 gtgtggcacg tgcgcgacgc acggctgcct cgactgctcg tgcggcacta ccagttgaag
     2521 tactgcgcga gcagctcgta gacgagcggt gcgctgagcg cgccggcaaa cgcgccgagc
     2581 attgcgagga acagcggcag aatgatgcgc cgccgtctgc gccgcatcac cattgccgca
     2641 ccaaccgcgg gccagcgtgg ctggagcgga cgcggtgcgc gcagctggag tggaggcggt
     2701 gcgcgcagct ggagcgcgcg catcggttgg tacacgaccg gcggcggggt gggttcgggc
     2761 ggcaacggca gcggtgctgg cgtctgggga cgttgcatcg ggaccggtgt ttggagcacc
     2821 ggagccagtt gcaccgtcgg catcgaaggc acgagcggcg gcggaaggat cggtttggca
     2881 tcgaccagcg acgcagcgct ggtccgcggc gcgggctgcg cggtgatcgg ccgactaccg
     2941 cgaacaggac gcgcggtctc gcccgcgagc ttgaacaccc actcgaccca ctccatcacc
     3001 tcggcgttcg acgacggctt ggcgacgccg gcgagcgcgt tgcgcagcgc ggtcgcgttc
     3061 tgccagcgca gcataggatc gcgctgcaac gcggtcatca cgatcgattc gagctcggcg
     3121 gggatgtcgt ggttgtatgt ggaaggcggc gtgatcggct ccgagcgcac cttgcgcatc
     3181 atctcgaagt catcatcggc atcgaacagg cgcgcgttgc agagcatctc gtacgcgacg
     3241 acgccaaccg cccacaaatc cgcgcggcga tcgaggccgg tgtcttcgag atattcgggc
     3301 gcgatgtagc cgagcttgcc cttgatgatg ccggcctgcg tgtgtgtgct gactgtcttc
     3361 gcgatgccga agtcgatcag cttcacgaca ccgttgctcg agatcacgat gttcgacggc
     3421 gacacgtcgc gatgcacgat gcgcagcggt ttgccgtgct cgtcgcagcg atcgtgcgca
     3481 tacgcgagcg catcgcagat ctggatcagg aggttgagcg cgatcgggta cggaatcagt
     3541 ccgacggttg cagcaccgtg gcgaagcagc tgcttgagcg tcgggcctgc aaccagctcc
     3601 atcgcgagga agtactcctc gccgaccgtg ccgaagtcgt agacctcggc gatgttgcga
     3661 tggcgcaaga gcgagacaag ccgcgcttca tcgaggaaga gcttcacgag atccggatcg
     3721 gcagccgcgt gcgggagcat gcgcttgagc gcgacccgct ggcgtacgcc tttgacccga
     3781 cgctcggcgc gatgcacgga cgccatgccg ccggtaccga gctgctcgcc aatcgagtac
     3841 tcgccgaagg tcgcggtcgg gtccggcgag tcattgcgcg gccatgcgcg tggggcggtg
     3901 atctcgcccg acaggatgat ggtcgacttc tcgtcgccga acgtctccgc ccggaggcca
     3961 cggcggacag aggcacgacc ctgtcccgac cactccactg gcggagtcca cacgtcgcgg
     4021 cgacccacgc aagctcatcg agcaaagcgc gagccacctc acagcgcgat tcgacgttga
     4081 agtttcaggc gcttggcgtg gtccgcagcg ggggatgggg cctcagggac ggcgagtgaa
     4141 atcgccgggt tggctatccg tgttttgcac acatcaaagc ggcggctcga tcgcgatgtc
     4201 ggtactcgcg gtcggcgccg ccagccgctc gcgcagggga tcgagcggcg ggagcttcag
     4261 cgccgcacgc tcgcgttcgc agatccgccc ccatccgtcg gcgaccagag agctgcgcac
     4321 ctcgcggatg cacgcctcgg tctcggtcgc cgcgttggcg gaatgcttcg tgacgcgcgc
     4381 gagctgagcg agacgtgcat gcgctgccgc acgcacgctc ggatcgctac tcatcgtcag
     4441 cttgcgatac ccaagcgcga catcgtcggc atgtgcaccg cgccggccgc ggacgacgtc
     4501 ttcgagctgg aagtcgagga gcatgcgcgt cacggtctcg ctgttgttcg atacgaaggc
     4561 gagctcgaga cgcacggcgc cggccatcac gctgccgata cgtcgctgta gacaca
//
LOCUS       DASUFX010000101         8333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_155847,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000101 DASUFX010000000
VERSION     DASUFX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155847"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..909)
                     /locus_tag="VFV99_03920"
     CDS             complement(100..909)
                     /locus_tag="VFV99_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5058479.1"
                     /translation="MSRIVAVANQKGGVGKTTTSINLAASIASRGYRVLLVDFDPQGN
                     ASSGVGYPRDKVELTVYDVLVGEVALEDVIRPTDITTLFVAPATTDLVGAEIELIGAE
                     HRERYLANALAPVANEYDYIVIDCPPSLGMLTLNALVAADGIVVPMQAEYFALEGLSA
                     LTATIEKVKAAYNPHLTIDGVLFTMFDGRMNLANQVRAEVAKHFGDKVLDTTVPRNVR
                     LSEAPSHGKPVLLYDLRCPGTKSYLAVADEFLDRTCQSLSLASRRERATVA"
     gene            1024..1209
                     /locus_tag="VFV99_03925"
     CDS             1024..1209
                     /locus_tag="VFV99_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058480.1"
                     /translation="MSSGIPRIYTSFAEFEREELRRLDSLHTSVDDLVEERFAEELDF
                     GQSDQPRRRGRPRKHPR"
     gene            complement(1210..2271)
                     /locus_tag="VFV99_03930"
     CDS             complement(1210..2271)
                     /locus_tag="VFV99_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058481.1"
                     /translation="MKRACLLFALAACTAEPAEINYTGETRRFVVDQIAVPLTTNEAR
                     ATGADLDGDRTIDNQLGMVTATLASVADDVTKHGNDMIASGVIASSVELVADDFTTDE
                     TVALRYFGADGDDAELIAGSLFDGTFEVSTKERGAGAVHLPVFVDADPVILPLVQMRA
                     RLVADGHGGFDATIQGAVPPEVARGAAYTSLVQMFDSYPSAHVAFFRMLDTQPYDFQI
                     SRAEFEANSLIKSLMAPDIEISDERLLSIGFRVHLAPCADGTCVTAPPADACFDRVQD
                     AGETGVDCGGPCHACEANATCFDGVRNGLETDVDCGSECGPCATGKDCWSGSDCVSGT
                     CGQPCSGTFCGTYTLDVCR"
     gene            complement(2418..4319)
                     /locus_tag="VFV99_03935"
     CDS             complement(2418..4319)
                     /locus_tag="VFV99_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5058482.1"
                     /translation="MSLARLVVIEGPDAGREFELRMRGTKVGRDEKCDVQLSDPTVSR
                     NHGLIELRDGALTFVAEKRTLINGAEISEHRLESGDEIVLGATKLAFLPADGVAVTKA
                     SSHVTMEVSSRQLLALTGRDDRARRHLAAIAQLGDRLRAESAAGRDAVARAACDAART
                     ALEAHRAFVLSAGKRPSPVAAAIGPSDTSQLQIPQEVIDKVINQRQVVTATISASAGG
                     RALIAAPVHGSADEVVAVLWIDRKGAEWDEADSLAAGCLAHLTGAAWVGADTRDQLSR
                     RAEALEERYSDGELIGRSPAAQRVVDFVNRVGPSDATILLGGESGSGKEMVARAIHRA
                     SRRAKGPCVAVNCAALTESLIESELFGHEKGAFTGATEKKAGRFEMADRGTLFLDEVG
                     ELPLGLQTKFLRVLEERRFERVGGQKAIEVDVRVVAATNRDLAEMVKRGTFREDLYYR
                     LSVIHIEVPPLRDRLDDVPLLADHFLTRFRHQAARRITGFAPDAIAAMTRYHWPGNVR
                     ELRNAVERAIVLGEREQIMAQDLPPQVLAQAAPPRTRNSPPTPPLGSAISTAVPSIAP
                     AITPAVTIDAAPPTALAGRAKSLRELEKEGILAALAATNGNKAQAAAILEIDRSTLYK
                     KLKDYDIEG"
     gene            4348..4782
                     /locus_tag="VFV99_03940"
     CDS             4348..4782
                     /locus_tag="VFV99_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058483.1"
                     /translation="MKRLVLAILLVAACSGSKQKQGDTTGSGSALYAKKILVAWGISQ
                     NGSSAEIFLQTTDETGKQTSYPVGTYEGTCQQIKPAEDMKALTGVNCTAGNGGIELHA
                     VVNDADIVVLKLKVQVGVTPDPMAREEVTRVKAPAGAAIEAA"
     gene            4956..6164
                     /locus_tag="VFV99_03945"
     CDS             4956..6164
                     /locus_tag="VFV99_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058484.1"
                     /translation="MDARVGDTQTVDAQIDAKVFMDAKVYMDAPPDAAGVCNPLTQTG
                     CNVGDKCTWLLDAVTPQYVGHIGCAPAGTANTGDACIYGAPGVTGYDGCKKGLVCGDY
                     RGGAGVCKQICDNQGGSPACGANTSCVTYSGLFSTGSTTPAAAGVCDPSCDPLADNDF
                     DGAGALTKTGTTCGSSATVGCYGYPSFGTPPITAWSCTSDINYLEAQPAGFRHRVQCT
                     ETSGCADPGPTLYVNSCNQGYLPLLRESTMVSTAVCVAMCKPHNCYLGSCGTNNDFRL
                     GDAPHRCNTSDAMGTFDTSAGGEHCRFIWSFEIDDNNTFLRSPSSDTVGFCFDHSKYL
                     YDSNNDGTADTPLPPCATLPNGTGSGTSFGAADLGCVDTANAGLPFTGKPHVANHLDL
                     RLLFHPTRAP"
     gene            6310..6618
                     /locus_tag="VFV99_03950"
     CDS             6310..6618
                     /locus_tag="VFV99_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058485.1"
                     /translation="MRTALLLMVVLGGCVADAEDPPPVKTVAGGDVTLLDEQDTEMDV
                     CALAAELPTEDICSLACDPPAMAARMVSDGMGEPGTCYQLYCALPDDAHVLVGVCLPP
                     "
     gene            6690..7901
                     /locus_tag="VFV99_03955"
     CDS             6690..7901
                     /locus_tag="VFV99_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058486.1"
                     /translation="MRLYLVAFFAVVACGDDGAAVHDAAPDTPDLCAKPASSCNPLSQ
                     SGCGAGEKCTWILDALMPQYAGHIGCARAGNGDIGAACTYRAPGCDGYDNCKQGLVCS
                     DFFGGAGVCKQVCDYQGGIPACDAQHSCVPYSGLFETETTMQAAGVCDVACNPLTDND
                     FDGAGTASTRTTNACGSDPTIGCYGYPSFGTFPKTEWSCTIDRNAAIAQPTGLRHRVL
                     CTTANGCADPSVYINSCNQGYLPLLRESTMVSTAICTALCKPKNCFAGSCGTGDIDRL
                     GAAPHRCQTPDRVGSFNTGADGEHCRFIWSFEIDTQGNFLRSSTSDTVGFCFDHSKYL
                     YDSNADNTPDTPLPACATLPDGFATTTSKVDGAADIGCVDTTHAQLAHGKAPAQPTFD
                     DLRPLFTRAVK"
BASE COUNT         1428 a         2720 c         2792 g         1393 t
ORIGIN      
        1 tcgacttgtc cgacgtacgg atcggcgacg gcgcagccgc cgtcggcaac agcgccgaca
       61 gaccacgccc gagcccacgc ttcttgagat ccgaggtcat catgcgactg ttgccctttc
      121 acggcgggac gccaaggaca atgattgaca tgtgcgatcg agaaactcgt ccgccaccgc
      181 caggtaactc ttcgtacccg gacagcgcaa atcgtagagc agcaccggtt tgccgtggct
      241 cggcgcctcc gagaggcgga cgttgcgcgg caccgtggtg tcgaggacct tgtcgccaaa
      301 atgcttggcc acctcggcgc ggacctggtt cgccaggttc atgcggccgt cgaacatcgt
      361 gaacaacacg ccgtcgatcg tgaggtgcgg gttgtacgcc gccttcacct tctcgatcgt
      421 cgcggtcagc gccgataggc cctcgagtgc gaagtattcg gcctgcatcg gtacgacgat
      481 gccgtccgcc gcgaccagcg cgttgagcgt gagcatgccg agcgatggcg ggcagtcgat
      541 gacgatgtag tcgtactcgt tcgcgacggg cgcgagcgcg ttcgccagat accgttcgcg
      601 gtgctcggcg ccgatcagct cgatctctgc accgacgagg tccgtcgtcg ccggcgcgac
      661 gaacagcgtc gtgatgtcgg tcggccggat cacgtcctcg agcgcgacct cgccaacgag
      721 cacgtcgtag acggtgagct cgaccttgtc gcgcgggtag ccgacgccac tcgacgcatt
      781 gccttgggga tcgaaatcga ccagcagcac gcgatagccg cgagaggcga tcgatgcagc
      841 gagattgatc gacgtcgtcg tcttgccgac gccccccttc tggttcgcta cagcgacgat
      901 gcgggacatg ttcagcggcg tatcacgcag ctatgacgat ccaaaaattt ggccctcgta
      961 tcgcgttcgg cgtaccttgt acgcagatgt aggacatgtg ggcatgtcct tgaaggagcg
     1021 ccaatgtcgt ctggaattcc gcgtatttat acttcgtttg ccgagttcga gcgcgaggag
     1081 ctgcgccggt tggattccct ccacacgtcg gtcgatgatc tcgtggagga gcgcttcgca
     1141 gaagagctcg acttcggtca gagcgaccag ccgcgccgtc gcggccgccc gcgcaagcac
     1201 ccgcgctgat cagcggcaga catccagcgt gtacgtgccg cagaaggtgc cgctgcatgg
     1261 ctggccgcac gtaccgctga cacagtccga accgctccag caatctttgc ccgtcgcgca
     1321 cgggccacat tcagatccgc aatcgacgtc ggtctcgagt ccgttgcgaa ccccgtcgaa
     1381 gcaggtcgcg tttgcttcgc acgcgtgaca cggcccgccg caatcgacgc cggtttcgcc
     1441 cgcgtcctgg acgcgatcga agcacgcgtc tgccggcggc gccgtcacgc acgtcccgtc
     1501 ggcacagggg gcgaggtgga cgcgaaaccc gatcgacaac agccgttcgt cgctgatctc
     1561 gatgtcgggc gccattaacg acttgatgag cgagtttgcc tcgaactcgg cgcgcgagat
     1621 ctggaagtcg tagggctgcg tgtcgagcat gcggaagaac gcgacgtgcg cgctcggata
     1681 cgagtcgaac atctgcacga ggctcgtgta cgccgcacca cgcgcgacct cgggtggcac
     1741 cgcaccttga atcgtcgcgt cgaagccgcc atggccatcg gcgaccagcc gcgcacgcat
     1801 ctgcacgagc ggcaggatga ccggatcggc gtcgacaaat accggcaggt gcacggcacc
     1861 ggcgccgcgc tccttcgtcg agacctcgaa tgttccgtcg aacagcgagc cggcgatcag
     1921 ctcggcgtcg tcaccatcgg cgccgaagta acgaagcgcg acggtttcgt cggtcgtgaa
     1981 atcgtccgcg acgagctcga ccgacgatgc gatcacgccg gatgcgatca tgtcgttgcc
     2041 gtgcttggtg acgtcgtcgg cgaccgacgc cagcgtggcg gtcaccatgc cgagctggtt
     2101 gtcgatcgtg cggtcgccgt cgaggtcggc accggtcgcg cgggcctcgt tggtcgtgag
     2161 cggcaccgcg atctgatcga cgacgaagcg gcgcgtctcg ccggtgtagt tgatctcggc
     2221 gggctcggcc gtgcaggcgg cgagggcgaa caaaaggcag gctcgtttca tggccgttcg
     2281 tgactgcagt catggtgcca ggtccgacca ttcgagaaac cgtgtacttg cggtcgggag
     2341 atcgacggtt gccggcgaat cgcgacgcgg atgtcgtggc ttcgccgaag gtgtcgagct
     2401 ttcaccgtcg acgacggtca accctcgatg tcgtagtcct tgagcttctt gtagagcgtc
     2461 gagcgatcga tctcgaggat cgcggcggct tgcgccttgt tgccattcgt tgccgcgagc
     2521 gcggcgagga tgccttcctt ctcgagctcg cgcagcgact tcgcgcgacc tgccagcgcc
     2581 gtgggtggag cggcatcgat cgtcaccgcc ggcgtgatcg cgggtgcgat cgatggaacg
     2641 gcggtcgaga ttgccgaacc gagcggcggt gtcggcggcg agttgcgggt gcgcggtggc
     2701 gctgcctgcg cgagcacctg tggcgggaga tcctgcgcca tgatctgttc gcgttcgccg
     2761 agcacgatcg cgcgctcgac cgcgttgcgg agctcgcgca cgttgcccgg ccagtgatag
     2821 cgcgtcatcg cggcgatcgc gtcgggcgcg aagccggtga tgcggcgcgc ggcttgatga
     2881 cggaagcgcg tgaggaagtg gtcggcgagc agcggcacgt cgtcgaggcg atcgcgcagc
     2941 ggcggcacct cgatgtggat cacgctgagt cggtagtaga ggtcctcgcg gaacgtgccg
     3001 cgcttgacca tctcggcaag gtcgcggttc gtagcggcga cgacgcggac gtcgacctcg
     3061 atcgccttct gcccgccgac gcgctcgaac ctgcgctcct cgagcacgcg gagaaacttc
     3121 gtctgcaagc cgagcggcag ctcgccgacc tcgtcgagga acagcgtgcc gcgatcggcc
     3181 atctcgaagc ggccggcctt cttctcggtc gcgccggtga atgcgccctt ctcgtggccg
     3241 aacagctcgc tctcgatcaa tgactcggtg agcgcggcgc agttgaccgc gacgcacggg
     3301 cccttcgcgc gccggctcgc ccggtgaatc gcgcgcgcga ccatttcctt gccggagccc
     3361 gactcgcccc cgagcaagat cgtcgcgtcg ctcggaccca cgcggttcac gaagtcgacg
     3421 acgcgctggg cggcgggcga ccgcccgatg agctcgccgt cgctgtagcg ttcctcgagc
     3481 gcctcggcgc gacgcgacaa ttgatcgcgc gtatcggcac cgacccacgc ggcaccggtg
     3541 aggtgagcga ggcagccggc ggcgaggcta tcggcctcgt cccactcggc acctttgcga
     3601 tcgatccaca gcaccgcgac cacctcgtcg gcactgccgt gcaccggagc agcgatcagc
     3661 gcgcggccgc ccgccgaggc tgagatcgtc gcggtcacga cctggcgctg gttgatcacc
     3721 ttgtcgatca cttcctgggg gatctgcagc tgcgacgtgt cgcttggccc gatcgcggcg
     3781 gcgaccggag acgggcgctt gcccgccgat agcacgaacg cgcggtgcgc ttcgagcgcg
     3841 gtgcgcgcgg catcacacgc ggcacgggcg acggcatcgc ggccggccgc agattcggcg
     3901 cgcaggcgat ctccaagctg cgcgatggcg gcgaggtggc ggcgggcgcg gtcgtcgcgg
     3961 ccggtgagtg cgagcagctg gcggctcgac acttccatcg tgacgtggct cgacgccttg
     4021 gtcaccgcga cgccatcggc cggcaggaac gcgagcttcg tcgcgccgag cacgatctcg
     4081 tcgcccgact cgagccggtg ctcggagatc tcggcgccgt tgatcagcgt gcgcttctcg
     4141 gcgacgaacg tcagcgcgcc gtcgcgcagc tcgatgaggc cgtgattgcg cgacacggtc
     4201 ggatcgctga gctgcacgtc gcatttctcg tcgcggccga ccttcgtgcc acgcatgcgg
     4261 agctcgaatt cgcggcccgc atccggacct tcgatgacga cgagtcgcgc caacgacatg
     4321 ccggcatctt agtgtaagtt tcgcgcgatg aagcgcctgg tgctcgcgat cctcctcgtc
     4381 gccgcctgca gcggcagcaa gcagaagcag ggcgatacga ccggcagcgg ttctgccctc
     4441 tatgccaaga agatcctcgt cgcgtggggc atctcgcaga acgggtcgtc ggccgagatc
     4501 ttcttgcaga ccaccgacga gaccggcaag cagacgagct acccggtcgg cacctacgaa
     4561 ggcacctgcc agcagatcaa gccggccgag gacatgaagg cgctgaccgg cgtcaactgc
     4621 accgcgggca acggcgggat cgagctgcac gccgtcgtca acgacgccga catcgtcgtg
     4681 ctcaagctca aggtgcaggt cggcgtcaca cctgatccga tggcgcgcga agaagtcacg
     4741 cgggtgaaag cacccgcagg tgctgcgatc gaagcggcat aaattgcact accccgacgg
     4801 cacggcgagc ccggtaacgg ccggcaacgc acgtgaagat cgcgtgaaca tcttccgggg
     4861 cgcgcgagcc atgcgacatt cgccggatgc gaagcgcggt tgtggcagtg gtattggttg
     4921 cggcgtgcgg cggcgacccc gacggaacgc gcccgatgga cgcacgggtt ggcgacaccc
     4981 agaccgtcga cgcccagatc gacgcgaagg tgttcatgga cgcgaaggtg tacatggacg
     5041 cgccgcccga tgcggccggc gtgtgcaatc cgctcacgca gaccggctgc aacgtcggcg
     5101 acaaatgcac gtggctgctc gacgcggtca cgccgcaata cgttggccac atcgggtgcg
     5161 cgccggcggg aaccgcgaac actggcgacg catgcatcta cggcgcaccc ggcgtcaccg
     5221 gctacgacgg ctgcaagaaa ggcctcgtgt gcggcgacta tcgcggtggc gcgggcgtct
     5281 gcaaacagat ctgcgacaac cagggcggca gtcccgcgtg tggtgcgaac acctcgtgcg
     5341 tgacgtactc ggggttgttc tccaccggct cgacgacgcc tgctgccgcc ggagtctgcg
     5401 atccgtcatg cgatccgctc gccgacaatg acttcgacgg tgcgggcgca ctcaccaaga
     5461 ccggcacgac ctgcggaagc tcggcgaccg tcggttgcta cggctatccg agcttcggta
     5521 caccgccgat cacggcgtgg agctgcacga gcgacatcaa ctacctggaa gcgcaacccg
     5581 ccggcttccg ccatcgcgtg cagtgcaccg agaccagcgg ctgcgccgat ccagggccta
     5641 cgctctacgt gaacagctgc aaccaaggtt acctgccgct attgcgcgag agcacgatgg
     5701 tgtcgaccgc ggtctgcgtc gcgatgtgca agccgcacaa ttgctacctg ggcagctgcg
     5761 gcaccaacaa cgacttccgc ctcggcgatg cgccgcaccg ctgcaacacg agcgacgcga
     5821 tgggcacgtt cgatacgagc gcgggcggcg agcactgtcg cttcatctgg agcttcgaga
     5881 tcgacgacaa caacaccttc ctgcgctcgc cgtcgagcga cacggttggc ttctgcttcg
     5941 atcactcgaa gtacctctat gacagcaaca acgacggcac ggccgacacg ccgttgccgc
     6001 cgtgcgcgac gttgcccaac gggacgggct cggggacgag ctttggcgct gccgatctcg
     6061 gctgcgtgga taccgcgaac gccggactgc cgtttaccgg caagccgcac gtggccaacc
     6121 acctcgattt gcgcctgttg ttccatccga cgcgcgcgcc gtgaacgcgc attagcgcag
     6181 cgccgacgct gttagcgcac accggatcta cggcgcggtc ggcagggttt catcgcgcag
     6241 tgagacgcgt gcacacacag gaacttgcac gcgccgatcg accgtctgta gcgtggaaga
     6301 tgggcgttcg tgcggacagc gttgctcttg atggtcgtgc tgggtggctg cgttgcagac
     6361 gcagaggatc cgccgccggt gaagaccgtg gctgggggcg atgtcacgct gctcgatgaa
     6421 caggacaccg agatggatgt ctgtgcgctc gccgccgagc tgccgaccga ggacatctgc
     6481 tcgctcgcgt gtgacccgcc ggcgatggcc gcgcgcatgg tgagcgatgg catgggcgag
     6541 cctggcacgt gctatcagct ctattgcgcg ctgccagacg atgcccacgt gctcgtcggc
     6601 gtttgtctgc cgccgtgacg cgtacgcgtt gcagcagctg ccagtcgtgg taatgcgggt
     6661 gcgcgaccat gcagcgcgat actcgaacca tgcggctgta cctcgtcgcc ttcttcgcgg
     6721 tcgtcgcgtg cggtgacgat ggcgctgcgg ttcatgacgc cgcacccgac acaccggatc
     6781 tgtgcgcgaa gccggcgagc tcgtgcaatc cgctctcgca gagtggctgc ggtgccggcg
     6841 agaaatgcac gtggatcctc gacgcgctca tgccgcagta cgcgggacac atcggctgtg
     6901 cacgcgcagg caacggcgac atcggtgcgg catgcacgta ccgtgcgcct ggctgcgacg
     6961 gctacgacaa ctgcaagcag ggtctcgtgt gcagcgactt cttcggcggc gcgggtgtgt
     7021 gcaagcaggt ctgcgattac cagggcggca ttcctgcgtg cgatgcgcag cactcgtgcg
     7081 tgccgtattc aggtctgttc gaaaccgaaa cgacgatgca agcggctggc gtttgcgacg
     7141 tcgcgtgcaa tccgctcacg gacaacgact tcgacggcgc aggcacggcg tcgacgcgca
     7201 cgacgaacgc gtgcggtagc gatccgacga tcggctgcta cggctatccg agcttcggca
     7261 cgttcccgaa aaccgaatgg agctgcacga tcgatcgcaa tgcggcgatc gcgcagccga
     7321 ctggcctgcg ccatcgcgtg ctgtgcacca ccgcgaacgg ctgtgccgac ccgagcgtgt
     7381 acatcaatag ctgcaaccag gggtacctgc cgctgctccg cgagagcacg atggtatcga
     7441 cggcgatctg cacggcgctg tgcaagccga agaactgctt cgctggcagc tgcggcacgg
     7501 gcgatatcga tcggctcggc gcggcaccgc atcgctgcca gacgccggat cgcgttggct
     7561 cgttcaacac aggtgctgac ggcgagcact gccgcttcat ctggagcttc gagatcgata
     7621 cccagggcaa tttcctgcgc tcgtcgacga gcgacaccgt cggcttctgc ttcgatcaca
     7681 gcaagtacct ctacgattcc aacgccgaca acactccaga tacgccgctg ccggcgtgcg
     7741 cgacgttgcc cgacggcttc gcgacgacaa cgagcaaggt cgatggcgca gcagacatcg
     7801 gctgcgtcga tacgacacat gcgcagctcg cgcacggcaa ggcacccgcg cagcccacat
     7861 ttgacgatct ccgcccgctg ttcacgcgcg cagtgaagta gatcgcacgc gacgcgctgt
     7921 cacggcgttg atgtggtggc gtgcgcgctg cgcgtgcgct gcaccacgcg gcgcgatgtc
     7981 ggcaacgcag gagtggtttg cggtggacag cggcagcgcg cgcgatcgat cacgcagcga
     8041 ttcattgtcg acgcaggagt ggagagcggt gaagagcgct gataactgcg atcgcgcgct
     8101 cgtagcatct gtccggaaac cggacgtcag caggcgcgtc gcacgcgatg cgacggcgca
     8161 gcggatagtg gtctcatgcg caaactcctc tgtatcgcga tcgcgctact cggcggctgt
     8221 ggtggttgtg acaaggaaaa attgcaccct gatccacccg acagcggtgg cgacgccatc
     8281 atcgatggct gcaacccgct cacccaagca ggctgtgcag ccaccgaaaa atg
//
LOCUS       DASUFX010000102        10475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1684366, whole genome shotgun sequence.
ACCESSION   DASUFX010000102 DASUFX010000000
VERSION     DASUFX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684366"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFV99_03960"
     CDS             <1..385
                     /locus_tag="VFV99_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058487.1"
                     /translation="RKRIAGLLSGLDVHYDLGAGHPLLGRRMPDLDVVTADGPRRVFA
                     LLHEARPVLLNLGEPLDIAAWADRVRRVDARYTGAWELPVLGAVAAPTAVLIRPDGHV
                     AWVGDGTDRGLRDALTTWFGPPTAT"
     gene            complement(389..1339)
                     /locus_tag="VFV99_03965"
     CDS             complement(389..1339)
                     /locus_tag="VFV99_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5058488.1"
                     /translation="MFLDGGLPPAMGCGAMDDDFDFKELSNAPLARVPWYFRLGRFLS
                     KRKIRGGDRLLSLARHRGLLDHLVVYSLGDVELRVPLWRPCNQWLEDDVRDYEAAFMR
                     ALSSAVCQLSGDVTLVDCGADIGTVSAHLVARCKNIKRVVAYEPNRAAYGVLERNLRA
                     MRVVADARHAAVGNFAGRGRLVRPPQDPSAHAMYVAPAKDGPIDVQRVDDLCLPSGAA
                     CVIKIDVEGTEASVVEGAAQTIRRAREVLVAFEAHPRVAQRVGRDPVEVMQTLLAIRR
                     NLTFLVDTTPAVTITADRPLFDQLPPNRVYNVIARSPAGT"
     gene            complement(1475..3298)
                     /locus_tag="VFV99_03970"
     CDS             complement(1475..3298)
                     /locus_tag="VFV99_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5058489.1"
                     /translation="MAAATQQDSRRQIVSRPPQPQPAIEDNALIGDLHTAGLVGKDGS
                     INFMCLPEFDDGSCFASLLGTPENGQWKIAPVRPLKSVKRRYRGESLILETEMETADG
                     IVRLTDFMPIRRGAPRVVRIIEGVRGSVTMQFQLHPRFAYGLTVPREETNGSVSSAIA
                     GPDAVYLRGGRGRGAPPFDAELTVNAGERVAYELAWARPYESVPPPLDIAAALRDTEA
                     FWQDWTSRIRLPNKYQALVMRSLITLKACTYRPNGAIVAAPTFGLPETPGGERNWDYR
                     FTWIRDSVLALNALMSAGLTGEAAAFGDWLIDAIGGAPEQFQIMYGIRGERILTEIPL
                     PWLSGHGGAKPVRIGNGAYDQFQLDVLGELAAVVFLFARSQGRLNPRARETMKSLCHV
                     VEQMWMKEDHGIWEMRGPTRSFTASKVAAWVTIDRWIRLIDMFELQEDKAPWEALRDK
                     IRVEILDKGFDPKRNTFTQYYGSPNVDASLLFIPMSGFLPATDPKVVGTVKAIEKELV
                     QDGLVLRYRTDASDDGLVGEEGVFLACSFWLADVYHMMGRRDDARRLFERVASLCNDV
                     GLLAEEYLTREKRQIGNFPQAFSHLALVHAAYLLSDGLPLP"
     gene            complement(3370..5082)
                     /locus_tag="VFV99_03975"
     CDS             complement(3370..5082)
                     /locus_tag="VFV99_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5058490.1"
                     /translation="MWPDEHRPGPRPLIMIIDDRPHGLSAMLDAIARRYGGDYRVLAH
                     LSAQAALDELARAKRDGEQVALIIADQWMPEMPGAELLLRAHEIHPHAQRALLVGWGD
                     ARANEVVLQGCALNQLDNFILKPWAPPEVHLYPVVNEFLAEWMRMHGPRLELVRIVSE
                     SPSPRGEELRDMVERNGIPHGYYTIDSDAGQDIVKRHGVDMARLPAVVMFDGRVLHDP
                     TNVALADSLGVPQITDCDCDLAIVGAGPSGLAAAVNAASEGLSTIVVEREAVGGQAGT
                     SSLIRNFLGFPRGISGADLAQRAYQQAWLFGAKYALARNAVGIRLTDGMRVVSLDDGT
                     QIRARALIVATGAAYRRLGVPQVDKFSGRGVFYTAGADVALALRGQDVVVCGGGNSAG
                     QAVVHLAKSVRRVVLAVRGKSLGEHMSAYLVDEIRRLRNVEVRLEAEVVDANGERALA
                     EVMLRERRNGSVDAVRATALFVMIGAVPYSDWLHGALERDANGFILTDHELSPRARRA
                     FGDRTPMLLETSMPGVFAAGDVRAGSTKRLASAVGEGAVAVRVVFDFLRADVTTARLT
                     ASAR"
     gene            5362..8199
                     /locus_tag="VFV99_03980"
     CDS             5362..8199
                     /locus_tag="VFV99_03980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058491.1"
                     /translation="MQRDSQDEATETLAEGPRRKSQKRSREQPTRLGRYVILSELGRG
                     GMSVVYVAYDPTLDRRVALKVVRGDKLTTAHRARLHREAQALARLSHPSVVTVFDVGD
                     LTDDTFVAMELIDGMSLRDWLKTKRTWREIIKVILAAGRGLAAAHAAGIVHRDIKPDN
                     IVIATSGAVKVVDFGLARDLGDRSIETGEHSGDDLDQSASSYDSGRHSGSISSSSGSS
                     RPLDTITQMGNVVGTPAYMPPEARSKRPEADERSDQFSLCVTIYEALYGQKPFKVSRK
                     GVLDRAEMPTVADDPEADMRTLAAAPPRDTDVPAWLQKVVSRGLAVEPGQRYPSVNAL
                     LADLDRDPQRTRRRIAAAAGALLAVAGVATFITSRVMPTEQVAAKPSCSTGDERINAV
                     WNPQRLAAMKASPAARGAPWVAGAIDAFGARVDKYAGEWKAMYVESCQATRVNGIQSE
                     EALDLRTACLDRHLESLGALVNLMADAKVETLRRAGEVVDNLPPVADCADVKALRLVV
                     KRPTDPKVIARLDAIDRNLAQLSALYAIGDVNKAIEVADRVIAESEAIGYAPPLAQGL
                     YWRGRSIADRTGGEEAVAMFDKTFSAALSAGEDQMAADAASRIAQEALFAAQLPEFDR
                     WQRITHAMANRVNAANIVAFSDQLGCMANHFYGRPKTRLACLQKLVARVGPNEWLVTT
                     LGIAASEAGKPAEAIHWLEKGVELARSENGPDHPRTLEMRAYLCKGLHELGDYKRSAD
                     ECRDALTRLQKSAPDDHLLIARIRMYLAESEIELQHPDVARALLEAAKGGGEDEVKLE
                     ATTTLTELSGAPGTVSAEAIAQHRAAVAENQKVFEPFNKRHPNIVAARHELGVALLRR
                     GDAPAALIELAKADTDADPAEISSLELAQIRYAHAEALFKARASDRAEARKLAAEALD
                     IYVKDAPDTERFRKERTAIEGFIAKLDAGAR"
     gene            complement(8196..8693)
                     /locus_tag="VFV99_03985"
     CDS             complement(8196..8693)
                     /locus_tag="VFV99_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058492.1"
                     /translation="MRTVHMTKKYITVALFVAACGGKAPATQKPETETPPPADMAFKD
                     MNADQRMAFMKHTVLPAMKQTFSAFDPKFANMNCKTCHGKGAEDGSFEMPNPDLPRLP
                     KPDAFMAFAQDPKHAPWVKFMIEKVEPEMANLLKMKPFDPQTNTGDFSCHACHLTEGE
                     EAKKD"
     gene            8961..10043
                     /locus_tag="VFV99_03990"
     CDS             8961..10043
                     /locus_tag="VFV99_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5058493.1"
                     /translation="MLALVLAGRWRVGRRVPAASRVEDELATAPTDRIIFDTTAVDAW
                     DSALVAFAARLLTVTAHTEVTVDRTGLPAGVQRLLALAEATPARATRATKPSLSFFER
                     VGLWSLGRHEHIGKTLEWIGLLTTAFAKFLVGRAKFRRSDLGLQMQLAGAHAIGIVSL
                     VAALVGLILAFIAAVQLQSFGAQLYVANLVGIGVVRELGAVMAAIVVAGRTGAAFAAE
                     LGTMRVTQEIDALTTFGVSPVEYLVLPRVLAVTLMMPLLCVYADLLGVLGGAAVGIGL
                     MHISPRIYYDQTIQAIDLADLLGGLLKAGTYGFLIGAAGCFTGLRAGRSAAAVGKATT
                     SAVVAGIVLVIVACGIYAVVFYRLRI"
     gene            10046..>10475
                     /locus_tag="VFV99_03995"
     CDS             10046..>10475
                     /locus_tag="VFV99_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5058494.1"
                     /translation="MASSPHIVVRDLTVSYGSYVVLHDVSFTVERGDVFVIMGGSGSG
                     KSTLLNAMIGLVEPVTGDVLYDDTSFTHADPDERRRISRKFGVLFQFGALFSGLTLAE
                     NVALPLEELTRLGRREIRELAELKLALVGLGSFTEFYPSEI"
BASE COUNT         1802 a         3577 c         3311 g         1785 t
ORIGIN      
        1 gcgcaaacgc atcgcagggt tgctctcggg tctcgacgtt cactacgacc tcggcgcggg
       61 tcatccactg ctcggtcgcc gtatgccgga tctggatgtg gtcaccgccg acggtccgcg
      121 ccgtgtgttc gcattgctgc acgaagctcg accggtgttg ctgaatctcg gcgagccgct
      181 cgacatcgca gcgtgggcgg atcgcgttcg ccgcgtcgac gctcgttaca caggcgcgtg
      241 ggagctcccc gtgctcggcg cagtcgccgc tccaaccgcc gtgctgatcc gaccggacgg
      301 ccacgttgcg tgggtcggcg acggaacgga tcgcggcctg cgcgatgcgt tgactacgtg
      361 gttcggaccg ccaaccgcga cgtagcgatc aggtgcccgc aggtgagcgc gcgatcacgt
      421 tgtagacgcg attcggcggt agctgatcga acagcggacg atcagcggtg atcgtgaccg
      481 cgggtgtcgt atcgacgagg aacgtcaggt tgcggcgaat cgcgagcagc gtctgcatca
      541 cttcgaccgg atcgcggcca acgcgctgcg cgacgcgcgg atgcgcttcg aacgcgacga
      601 gtacctcgcg ggcgcgtcga atcgtctgtg cggcaccctc gacgacggaa gcctcggtgc
      661 cttcgacatc gatcttgatc acacacgccg caccactcgg taggcacaga tcatcgacgc
      721 gctggacgtc gatcggccca tccttggcgg gcgccacgta catcgcgtgt gcgctcgggt
      781 cctgcggcgg acgcacgagt ctgccgcggc ctgcgaagtt gccgactgct gcatgacgcg
      841 catcggcgac cacgcgcatc gcgcgcaggt tgcgctcgag cacgccgtag gccgcgcgat
      901 tgggttcgta cgcgacgacg cgcttgatgt tcttgcaccg cgcgaccaga tgcgcagaca
      961 ccgtaccgat gtcggcgccg caatcgacga gggtgacgtc tcccgagagc tggcacacag
     1021 cggagctgag tgcgcgcatg aacgcagcct cgtaatcacg cacgtcgtct tcgagccatt
     1081 ggttgcacgg gcgccacagc ggcacccgga gctcgacgtc accgagcgaa tacacgacga
     1141 gatgatcgag gaggccgcga tgacgcgcta atgacaacag ccgatcgcca ccgcggatct
     1201 tgcgcttcga caagaagcgt ccgagtcgga agtaccaagg tacccgcgcg agcggcgcgt
     1261 tcgagagctc cttgaaatcg aagtcgtcat ccatggcacc gcagcccatt gcaggcggca
     1321 gaccaccgtc gagaaacacc gtcgaacgtc gcgcgccgtc acgaccggct acgtgtgcac
     1381 acggcgaagc gtccctcggg ggctcgcgct gcgggctctg cgaacggttt gttccatcgc
     1441 gcttgtgtca cagcgggcgc gatggcgctc ggcatcacgg cagcgggaga ccgtcgctga
     1501 gtagatatgc ggcgtggacc agcgcgaggt ggctgaacgc ctgcgggaag ttgccgatct
     1561 gtcgcttctc gcgcgtcagg tattcctcgg caagcaggcc gacgtcattg cacaggcttg
     1621 cgacccgctc gaagagacgg cgcgcgtcat cgcggcgtcc catcatgtga tagacgtccg
     1681 cgagccagaa cgagcacgcg aggaacacac cctcctcgcc caccaggccg tcgtcgctcg
     1741 cgtccgttcg ataacgcaga acgaggccgt cctgcacgag ctccttctcg atcgctttca
     1801 ccgtaccgac gactttcgga tcggtggcgg gcaagaagcc gctcatcggg atgaacaaga
     1861 ggctcgcatc gacgtttggc gatccgtagt actgcgtgaa cgtgttgcgc ttcggatcga
     1921 agcccttgtc gaggatctcg acgcggatct tgtcgcgtag cgcttcccac ggcgccttgt
     1981 cttcttggag ctcgaacatg tcgatcaggc ggatccagcg atcgatcgtc acccatgcgg
     2041 cgaccttcga cgccgtgaat gatcgtgtcg gcccacgcat ctcccagatg ccgtggtcct
     2101 ccttcatcca catctgctcg acaacatggc acaacgattt catcgtctct cgcgcacgcg
     2161 gattcaaccg accctgtgat cgcgcgaaca ggaagacgac tgcggcaagc tcgccgagca
     2221 cgtcgagctg gaactgatcg tacgcgccat ttccgatgcg caccggcttc gcgccgccgt
     2281 ggcccgacag ccatggcaac gggatctcgg tgaggattcg ctcgccgcgg atgccgtaca
     2341 tgatctggaa ctgctcgggt gcaccaccga ttgcatcgat caaccagtcg ccgaacgccg
     2401 ctgcttcgcc ggtcaggccc gcggacatca gagcgttgag cgcgagcacg gagtcgcgga
     2461 tccacgtgaa gcgatagtcc cagttgcgtt cgccgcccgg cgtttccggc aagccgaacg
     2521 taggcgctgc gacgatcgcg ccgttcggcc gatacgtgca cgccttcagc gtgatcagtg
     2581 accgcatcac gagcgcttgg tacttgttcg gcaaccggat ccgagacgtc cagtcctgcc
     2641 agaacgcctc ggtgtcgcgc aaagccgcag cgatatcgag cggcggcggt accgattcgt
     2701 acggccgcgc ccacgccagc tcgtatgcga cccgctctcc cgcattgacc gttagctcgg
     2761 cgtcgaacgg cggcgcaccg cggccgcgcc caccgcgcag gtacacggcg tcggggccgg
     2821 cgatcgccga tgacaccgag ccgttcgtct cctcgcgtgg cacagtcagg ccgtacgcaa
     2881 agcgcggatg cagctggaac tgcatcgtca ccgaaccgcg cacgccctcg atgatgcgca
     2941 cgacacgcgg agcacctcgt cggatcggca tgaaatcggt cagccgcacg atgccgtcgg
     3001 ccgtttccat ctcggtctcg aggatcagcg attcgccacg gtagcgccgc ttcaccgact
     3061 tcagcggacg cacgggtgcg atcttccatt gcccgttctc cggtgtgccg agcagcgacg
     3121 cgaagcagga accatcgtcg aattccggta ggcacatgaa gttgatcgag ccgtccttgc
     3181 cgaccaaccc cgccgtatgc agatcaccga tcagtgcgtt gtcctcgatc gccggctgcg
     3241 gctggggagg acgcgaaacg atttggcgac gactgtcctg ctgcgttgct gctgccatgg
     3301 cgcaccatct ggttgttcac gacaacccta cggcccgtac tcgttgctgc aatcgcgtga
     3361 gctacgcagt caccgcgcgc tcgcggtgag ccgtgcagtc gtgacatcgg cgcgcagaaa
     3421 gtcgaacacg acgcgcaccg cgacggcacc ttcgccgacg gccgaagcaa gtcgcttcgt
     3481 cgagcccgcg cgcacatcac cagccgcaaa cacgccgggc atgcttgtct ccagcagcat
     3541 cggcgtgcga tcaccgaacg cgcggcgcgc gcgcggcgac agctcgtggt ccgtcaggat
     3601 gaaaccgttg gcgtcgcgct ccagcgcacc atgcagccaa tcgctgtacg gcaccgcacc
     3661 gatcatcacg aacagtgcag tggccctcac ggcgtcgacg ctgccattac ggcgttcgcg
     3721 caacatgact tcggcaagcg cacgctcgcc gtttgcgtcg acgacttcgg cctccagccg
     3781 cacttctacg ttgcgtagcc gccggatctc gtcgacgaga tacgcagaca tgtgctcgcc
     3841 aagcgactta ccgcgcaccg caagcacgac gcggcgcaca ctcttcgcga ggtggacgac
     3901 cgcctgccct gccgagttgc caccgccgca caccacgaca tcctgaccgc gtaacgcgag
     3961 cgcaacgtct gcgccggccg tgtagaacac accacggccg gaaaacttgt cgacttgcgg
     4021 cacaccaagc cgccgatacg ccgcaccggt tgccacgatc agtgcgcgcg cccggatctg
     4081 cgtaccgtcg tcgagcgaga cgacacgcat accgtcggtg agccgaatgc cgacagcgtt
     4141 gcgcgccagt gcgtacttcg caccgaacaa ccacgcctgt tgatacgcgc gctgtgcgag
     4201 gtcggcgccg ctgatgccgc gcgggaagcc gagaaagttc cggatcagcg agctggtgcc
     4261 ggcttgtccg ccaaccgcct cgcgctcgac cacgatcgtg ctcaagccct ccgatgcagc
     4321 attgacggcg gcagcgagtc cgctcggccc ggcgccgacg atcgcgaggt cacaatcgca
     4381 gtcggtgatc tgcgggacgc cgagcgagtc ggccagtgcg acgttggtcg gatcgtgcag
     4441 cacgcggcca tcgaacatca cgacggccgg cagccgcgcc atatcgacgc cgtgcctctt
     4501 cacgatgtcc tgccccgcgt ccgagtcgat cgtgtagtac ccgtgcggga tcccattgcg
     4561 ctcgaccatg tcgcgaagct cttcgccccg cggcgacggc gactcgctca cgatccgcac
     4621 cagctcgagc cgaggtccgt gcatccgcat ccactcggca aggaactcgt tgaccacggg
     4681 atagagatgc acctcgggcg gcgcccacgg cttgaggatg aagttgtcga gctggttgag
     4741 cgcgcagcct tgcaggacga cctcgttcgc gcgcgcgtcg ccccagccga cgagcaatgc
     4801 gcgttgcgcg tgcggatgga tttcgtgcgc gcgcaacagc agctcggcgc ccggcatctc
     4861 cggcatccat tgatcggcga tgatgagcgc gacctgttct ccgtcgcgtt tcgcgcgagc
     4921 gagctcgtcg agcgcggctt gcgctgacag gtgcgcgagc acgcgataat cgccaccgta
     4981 gcgccgcgcg atcgcgtcta gcatcgctga caatccgtgc ggccggtcgt cgatgatcat
     5041 gatcagcgga cgcggaccgg ggcgatgctc atcgggccac atacgcgcaa gctatcgccg
     5101 caaatcgcag gaacaatcgc gcgcccgacg cacggcggct cgtccaaacg cctaccgtcg
     5161 ctacgcaaac cgcgcgtgac tttcggtacc cgagcccgca ccctttgcgt cgagtgatcg
     5221 ctcaggcctc gttagaaggg tcatgccagc aacactggcg agccggtcag cggaaacgat
     5281 cgatgcccac ataggccggt ctgggccgtt cgtgtagcgt ggagagtggc ggggttttgc
     5341 cgtcacttct gcggttgttt catgcagcgt gattcccagg acgaagctac cgagacgctg
     5401 gccgagggcc cacgtcgcaa atcacagaag cgtagtcgtg agcaaccaac gcgtctcggt
     5461 cgctatgtga tcttgtccga gctcggtcgc ggcggcatga gcgtcgtcta cgtcgcctac
     5521 gatccaacgc tcgaccgccg cgttgcgctc aaggtcgtcc gcggcgacaa gctgaccact
     5581 gcgcatcgcg cacgcctgca ccgcgaagcg caagcgctcg cgcgcctgtc ccacccgtcg
     5641 gtcgtcacgg tcttcgatgt cggcgatctc accgacgaca cgttcgtcgc gatggaactg
     5701 atcgacggca tgagccttcg cgattggctg aagaccaagc gcacgtggcg cgaaatcatc
     5761 aaggtgatcc tcgctgctgg ccgaggcctc gccgctgcac acgccgccgg catcgttcac
     5821 cgcgacatca agcccgacaa catcgtgatc gcgacgagcg gtgcagtgaa ggttgtcgac
     5881 ttcggtctcg cgcgcgacct cggcgatcgc tccatcgaga ccggcgagca ctcgggcgac
     5941 gatctcgatc agtcggcgag cagctatgac agcggtcgtc actccggcag catttcgagt
     6001 agcagcggct cgtcgcgccc gctcgacacg atcacgcaga tgggcaacgt cgtcggcacg
     6061 ccggcgtaca tgccacccga agcgcgcagc aaacggcccg aggccgacga gcgctcggat
     6121 cagttcagcc tctgcgtcac gatctacgaa gcgctgtacg ggcagaagcc gttcaaggtc
     6181 tcgcgcaagg gcgtgctcga tcgcgcggag atgccgacgg tcgccgacga tccagaagcc
     6241 gacatgcgca cgctcgctgc cgcgccgccg cgtgacactg acgtgcccgc gtggctccag
     6301 aaggttgtca gccgcgggct cgcagtcgaa cccggccagc gctatccgtc ggtcaatgcc
     6361 ctcctcgccg atctcgatcg cgatccacag cgcacgcgtc gccggattgc cgccgctgcc
     6421 ggcgcgctcc tcgcagttgc cggcgtcgcg acgttcatca cctcacgagt gatgccgacc
     6481 gaacaggtcg ccgcgaagcc gagctgcagc actggtgacg aacgcatcaa tgccgtgtgg
     6541 aatccgcaac gcctcgcggc gatgaaggcg tcgccggctg cacgtggcgc gccgtgggtt
     6601 gccggcgcga tcgatgcatt cggtgcgcgc gtcgacaagt acgctggcga gtggaaagcg
     6661 atgtacgtcg agtcgtgcca ggcgacgcgc gtcaacggca tccaatcgga agaggcgctc
     6721 gacctgcgga ctgcgtgtct cgatcgccat ctcgagagcc tcggcgcgct cgtgaatctg
     6781 atggcggacg caaaggtgga gacgctgcgg cgcgccggcg aagtcgtcga caacctgccg
     6841 ccggtcgccg actgcgcgga cgtcaaggcg ctgcgcctcg tcgtcaagcg gcccaccgat
     6901 cccaaggtca tcgcgcgcct cgatgcgatc gaccgcaacc tggcgcagct ctccgcgctc
     6961 tacgcgatcg gtgacgtcaa caaggcaatc gaggtcgccg atcgtgtgat cgcggaatcc
     7021 gaggcgattg gctatgcacc accgctcgcg cagggactct actggcgcgg gcgctcgatc
     7081 gcggatcgca cgggcggcga agaggccgtc gcaatgttcg acaagacgtt ctcggccgcg
     7141 ctcagcgccg gcgaggacca aatggctgcc gacgccgcct cgcgaatcgc gcaagaggcc
     7201 ctgtttgccg cacagctccc cgagttcgat cgctggcagc gcatcaccca cgcgatggca
     7261 aaccgcgtca acgcggccaa catcgtggcg ttcagcgatc agctcggctg catggcgaac
     7321 cacttctacg ggcgaccgaa gacgcggctc gcgtgtctgc agaagctcgt cgcgcgcgtt
     7381 ggcccgaacg agtggctcgt caccacgctc ggcattgccg cgagcgaagc gggtaaaccg
     7441 gccgaggcca tccattggct cgaaaaaggc gtcgagctgg cacgctctga gaacggcccc
     7501 gatcacccgc gcacactcga gatgcgcgcg tatctttgca aaggcctgca cgagctcggc
     7561 gactacaagc gctctgccga tgaatgtcgc gatgctttga cccgcttgca gaagtcagcg
     7621 cccgacgatc acctgctaat cgcacgcatc cgcatgtacc tcgccgaatc cgagatcgag
     7681 ctccagcacc ccgacgtcgc gcgcgcactg ctcgaagcag cgaagggcgg tggcgaagac
     7741 gaggtcaagc tcgaagccac gacgacgtta acggagctct cgggcgcacc cggcaccgtc
     7801 agcgccgaag cgatcgccca gcatcgcgcc gccgtcgccg agaatcagaa ggtgttcgaa
     7861 ccgttcaaca agcgtcaccc gaacatcgtc gcagctcgtc acgagctcgg cgtcgcgctg
     7921 ctcaggcgcg gtgacgcgcc ggccgccctc atcgagctgg cgaaggcgga taccgacgcg
     7981 gaccccgcgg agatcagctc gctcgagctc gcccagatcc gctatgcgca cgcggaggcg
     8041 ctgttcaagg cgcgcgcgag tgaccgcgcc gaagcgcgca agctcgccgc cgaagcgctc
     8101 gatatctacg tcaaggacgc gccagacacc gaacgcttcc gcaaggagcg caccgcgatc
     8161 gaaggcttca tcgcgaagct cgacgcgggc gcgcgctagt ccttcttcgc ttcttcgcct
     8221 tcggtgaggt gacaagcgtg gcagctgaag tcgcccgtgt tcgtctgcgg atcgaacggc
     8281 ttcatcttga gtaggttcgc catctcgggt tcgaccttct cgatcatgaa cttcacccat
     8341 ggcgcgtgct tcggatcctg cgcgaacgcc atgaacgcgt cgggctttgg tagtcgggga
     8401 agatccgggt ttggcatttc gaacgagccg tcctccgcgc ctttgccgtg acaggtcttg
     8461 cagttcatgt tcgcgaattt cggatcgaac gcgctgaacg tctgcttcat cgccggcagc
     8521 acggtgtgct tcatgaaagc catgcgctgg tcagcgttca tgtccttgaa cgccatgtcc
     8581 gcgggcggcg gcgtttcggt ttccggcttc tgggttgcgg gtgctttgcc gccgcaggcc
     8641 gcaacaaaca acgctacggt gatgtacttc ttcgtcatgt gtacggtcct cacgcctgtg
     8701 catagcgaag tgcgcgggcg tcgctccagt ggctgctgtt ccaaaatctg aaacgctgac
     8761 ttcctcgatc tccccgagcg aaccggttgc aaaaccgcat ggacgcgcta gccgccgccc
     8821 cttggcgcac gcgcgctgcc gcgagcaccg tagcgacgcc gagcgtgcca ccagggaacg
     8881 attgctgcat cacgtagtac cgcgccatgc agtcgtctca cgcggcacct cagctgacga
     8941 tgacgcgacc agaaccgcgc atgttggcgc tcgtgctcgc gggcaggtgg cgcgtcgggc
     9001 ggcgagtacc tgccgcctcg cgcgtcgagg acgagctcgc gaccgcgccc accgaccgca
     9061 tcattttcga cacgaccgcc gtcgacgcgt gggactcggc gctcgtcgcg ttcgctgccc
     9121 gcctcctgac cgtcacggcg cacacggagg tgaccgtcga ccgcaccggt ctaccggccg
     9181 gcgtccagcg cctgctcgcg ctcgccgaag cgacacctgc acgcgcgacg cgagcgacga
     9241 agccatcgct ctcgttcttc gagcgcgtcg ggctgtggtc gctcggtcgc cacgagcaca
     9301 tcggcaagac gctcgaatgg atcggcctct tgaccacggc attcgcgaag ttcctcgtcg
     9361 gacgcgcgaa gtttcgccgc tcggacctcg ggctccagat gcagcttgca ggcgcccatg
     9421 cgatcggcat cgtcagtctc gtcgccgctc tggtcgggct cattctcgca ttcatcgccg
     9481 cggtgcaact gcagtcgttc ggcgcacagc tgtacgtcgc gaacctcgtc ggcatcggcg
     9541 tcgttcgcga gctcggtgcc gtgatggccg cgatcgttgt cgccggccgc accggcgccg
     9601 cgtttgctgc cgagctcggc acgatgcgcg tcacccagga gatcgacgcg ctgaccacgt
     9661 tcggtgtatc gccggtcgag tacctcgtcc tgccgcgcgt gctcgcggtg acgctgatga
     9721 tgccgctctt gtgcgtctat gccgatctgc tcggcgtgct cggcggtgcc gcggtcggca
     9781 tcggcctgat gcacatctcc ccgcgcatct attacgacca gacgatccag gcgatcgatc
     9841 tggccgatct gctcggtggg ctgctcaagg ccgggaccta tggctttctg atcggcgccg
     9901 ccggctgctt cacgggccta cgtgccggac gttcggcggc cgccgtcggc aaggccacga
     9961 cctccgctgt tgtcgccggc atcgtgctgg tgatcgtcgc ctgcggcatc tacgccgtcg
    10021 tcttctaccg ccttcgcatc tagtcatggc ctccagtcca catatcgtcg ttcgtgatct
    10081 aaccgtcagc tacggcagct acgtcgtgct gcacgatgtc agcttcaccg tcgagcgcgg
    10141 tgacgtcttc gtgatcatgg gcggcagcgg cagcggcaag agcacgctgc tcaacgcgat
    10201 gatcggcctc gtcgagccag tcaccggcga cgtcctctat gacgacacga gcttcacgca
    10261 cgccgacccc gacgagcgcc gacgcatctc gcgcaagttc ggcgtgttgt tccagttcgg
    10321 cgcgctgttc agcggcctga cgctcgccga gaatgtcgct ctcccgctcg aggagctgac
    10381 ccggctcggc cgccgcgaga tccgagagct cgccgagctc aagcttgcgc tcgtcgggct
    10441 cggaagtttc accgagttct acccgagtga gatca
//
LOCUS       DASUFX010000103         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_433872,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000103 DASUFX010000000
VERSION     DASUFX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433872"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..790)
                     /locus_tag="VFV99_04000"
     CDS             complement(17..790)
                     /locus_tag="VFV99_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058495.1"
                     /translation="MRGLLTIARYELTRRWALWLVMAGVGFLARILASFTYDDRLFNV
                     CVVGAVVLSWVVAFIVGMSLFGRALHDGRLGFFFTRPIHGVVIAAGKLVGGLVLILGM
                     EVLLLIPLFSSDFTTTGDDGPRFVIGIALAFLAAGLVVGILARSRSRWFIADAIGGAI
                     AVLLAIIVFGESNAAQRYAIVQKWTPEEAGPMLGRIDTLMRGLAIAAIVVVLAAVTAA
                     ITLGRTDRERVHRSLAMTLWSSLAVTGLVGVAFAYWGLL"
     gene            complement(787..1539)
                     /locus_tag="VFV99_04005"
     CDS             complement(787..1539)
                     /locus_tag="VFV99_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5058496.1"
                     /translation="MDNVTVRIGGRTLLDRLSLTVKTGSVFALLGRNGAGKTTMFRCL
                     LGLRRPDEGRVLLFGRDAWTDRAAAMARLGVVPEEPDVPLDMTPLALARFCAPLYARW
                     NSDTFSSRLRHFGIREKTPFGRLSRGQKTQVMLAAALCASPEALVLDDPTLGLDPIAR
                     RELLAELVRELADRGTTTLVSTHDFAAVEGLATHIGVLRDGRLVLSGELEAIKTSYGQ
                     VDATPQRASGASPASLEEVFNDIVERQRGVAA"
     gene            complement(1557..1967)
                     /locus_tag="VFV99_04010"
     CDS             complement(1557..1967)
                     /locus_tag="VFV99_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5058497.1"
                     /translation="MNRKTLKLDPASATPLWRQLEESIRQLVGSGAWEPGEAVPSVRT
                     LATELLVNPATIAKAYQRLVDLGVLVVRRGEGTFVAAKPPILARAERTRTLREGAERF
                     ASLTATIGAEPEEAHAALREAFKGYVRVSAGAKS"
     gene            complement(2016..3932)
                     /locus_tag="VFV99_04015"
     CDS             complement(2016..3932)
                     /locus_tag="VFV99_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5058498.1"
                     /translation="MSTNRPAPPPASSSARSAGPPMRGPMGMTGPPQKAKNFGPSMRR
                     LIGLLAPQRKLIVFVLAFAIASVSLSVAGPKVLGHATDIVFKGFVAHGGHVNTALASN
                     MNVHAGEGIDFDALAHTLILVMALYIGASVLMWLQSYILNYVVQRTIRKLRADVDAKL
                     MRVPLAYFDRQPHGEVLSRVTNDIDNVAMALQQTMSQILIALLTVVGILVMLVWISPL
                     LSVIALVTVPISVMAAAKIGKRAQKGFIAQWKHVGVLNGQIEEAFTGHALVKVFGRRK
                     QIEDRFRAKNDELFDAGFRAQFISSLMMPVINFVGNLTYITIAVVGALRVATGKILLG
                     SVQAFIQYSRQFTQQLSQLAQVSNILQSGVASAERVFELLDEADQSPDRAAAFLPHPL
                     RGEVRFEDVSFRYKPEVPLIEKLSLVASAGQTVAIVGPTGAGKTTLVNLLMRFYDLDD
                     GAIRVDGVDIATAPRADVRARFGMVLQDTWLFGGTIRDNIKYGSPKATEDDMLAAAKA
                     TYVDRFVRALPDGYDTVIDEESSNLSTGEKQLITIARAFLANPAILILDEATSSVDTR
                     TEVLVQRAMATLRERRTSFVIAHRLSTIRDADIILVMEQGKIVEQGTHEQLLAAGGAY
                     HTLYHAQFAAPAAA"
     gene            complement(3929..>4119)
                     /locus_tag="VFV99_04020"
     CDS             complement(3929..>4119)
                     /locus_tag="VFV99_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058499.1"
                     /translation="RLAKTAVIVVAQRVASIRHAHQIVVLDGGKIVGKGTHDQLVTTC
                     PTYAEIVTSQTEEQEAAA"
BASE COUNT          737 a         1352 c         1356 g          674 t
ORIGIN      
        1 atgccaacgc tgtgtttcat agcaggcccc aatatgcgaa cgcgacgccc acgagtcccg
       61 tcacggcgag cgacgaccac aacgtcatcg cgagcgagcg atgcacgcgc tcgcgatccg
      121 tgcgaccgag ggtgatcgcg gctgtcaccg cggcgagcac gacgacgatc gccgcaatgg
      181 caagaccgcg catgagcgtg tcgatccgac cgagcatggg tcccgcttcc tccggggtcc
      241 acttctggac aatcgcgtaa cgctgcgccg cattggattc gccgaacacg atgatcgcga
      301 gcagcacggc gatcgctcca ccaatcgcgt cggcgatgaa ccagcgcgac cgtgaccgtg
      361 ccaggatccc gacgacgagc cccgccgcga gaaaggcgag ggctatgccg atcacaaacc
      421 gcggtccgtc atccccggtg gtcgtgaagt cagagctgaa caaggggatc aggagcagca
      481 cctccatccc aagaatcagg acgagcccac cgacgagctt gccggccgcg atgaccacac
      541 cgtggatcgg gcgcgtgaag aagaacccca gccgtccgtc gtgcaacgca cgcccgaaca
      601 gcgacatgcc aacgatgaac gcgacgaccc acgataggac gacggcaccg accacgcaga
      661 cgttgaacag ccgatcgtcg tacgtgaacg atgccaagat cctcgcgagg aatccgacgc
      721 cggccatgac caaccacagt gcccagcgcc gcgtcagctc gtagcgagcg atcgtgagca
      781 ggcccctcac gctgcgactc ctcgttgccg ctcgacgatg tcattgaaga cctcctcgag
      841 cgaggcaggc gaggcacccg acgcacgctg cggcgttgca tcgacctggc catacgatgt
      901 cttgatcgct tcgagctcgc cgctgagcac cagccgaccg tcgcgcagca ctccgatgtg
      961 cgtggcgagc ccttccaccg cggcgaagtc atgcgtcgac acgagcgtcg tcgtgccgcg
     1021 atcggcgagc tcgcggacaa gctccgccag cagctcgcgc cgcgcgatcg ggtcgagccc
     1081 gagcgttgga tcgtcgagca cgagcgcctc ggggctcgcg cacagcgcgg ccgcgagcat
     1141 gacctgcgtc ttctggccgc gcgatagccg cccgaacgga gtcttctcgc ggatgccgaa
     1201 gtggcgcagg cggctcgaga acgtgtcgct gttccagcgc gcatagagcg gagcgcagaa
     1261 gcgcgcgagc gcgagcggtg tcatatcgag cgggacgtct ggctcttccg gcacgacacc
     1321 caagcgagcc atcgccgcgg cgcgatccgt ccatgcgtcg cgcccgaaca gcagcacgcg
     1381 cccctcgtcg ggacgccgca aaccgagcaa gcagcgaaac atggtcgtct tgccggcgcc
     1441 gttgcgcccg agcagcgcga acaccgatcc ggtcttcacg gtcagcgaca gccgatccag
     1501 cagcgttcgc ccgccgatcc gaaccgttac gttgtccaag accaacggct ctgtcgtcat
     1561 gacttagctc ccgcgctcac acgcacgtag cccttgaatg cttcgcgaag cgccgcgtgt
     1621 gcctcctcag gttccgcccc gatcgtcgcc gtcaacgacg cgaacctctc cgcaccttcg
     1681 cggagcgttc gagtccgttc ggctcgcgcg aggatcggcg gcttcgctgc gacgaacgtc
     1741 ccctctccgc gccgaacgac gaggacgccg aggtcgacca atcgctgata cgccttggcg
     1801 atcgtcgcgg gattgaccag cagctcggtc gcgagcgtgc gcacggaagg aacggcctca
     1861 cccggctccc acgcaccgct accgacgagc tgccgaatgg attcctcgag ctggcgccac
     1921 agcggcgtcg cgctcgcagg atcgagcttc agcgttttcc ggttcactgt atcagtacag
     1981 tgatacaccg gaccggtgat tgtcaactgt cttcgttacg cggcggcggg cgcggcgaac
     2041 tgcgcgtgat agagcgtgtg gtacgcgcca cccgcagcga gcagctgctc gtgcgtgccc
     2101 tgctcgacga tcttgccctg ttccatgacg aggatgatgt cggcgtcacg gatcgtcgag
     2161 agccggtgcg cgatcacgaa gctcgtgcgc cgctcgcgca gcgtcgccat cgcgcgttgc
     2221 acgagaacct cggtgcgggt gtcgacggac gatgtcgcct cgtcgaggat caggatcgcc
     2281 gggttcgcga ggaacgcgcg cgcgatcgtg atcagctgct tctcgccggt cgacagattc
     2341 gagctctcct cgtcgatgac ggtgtcgtag ccatcgggaa gcgcgcgcac gaaacgatcg
     2401 acatacgttg cctttgcggc cgcgagcatg tcgtcttcgg tggccttcgg gctgccgtac
     2461 ttgatgttgt cgcggatcgt gccgccgaac agccaggtgt cttgcagcac catgccgaag
     2521 cgtgcgcgga cgtcggcgcg cggcgcggtc gcgatatcga cgccgtcgac ccggatcgcg
     2581 ccatcgtcca ggtcgtagaa gcgcatgagc agattgacga gcgtcgtctt gccggcaccg
     2641 gtcgggccga cgatcgcgac ggtttggccg gcggacgcga cgagcgacag cttctcgatc
     2701 agcggcacct cgggtttgta acggaacgag acgtcctcga agcggacctc accgcgtagc
     2761 ggatgcggca gaaatgccgc cgcgcgatcg ggtgactggt cggcctcgtc gagcagctcg
     2821 aacacgcgct cggccgatgc gacgccggac tgcaggatgt tcgagacctg cgcgagctgg
     2881 ctgagctgtt gcgtgaactg acgcgagtac tggatgaagg cttggacgct gccgagcagg
     2941 atcttgcccg tcgcaacgcg cagcgcgccg accacggcga tcgtgatgta cgtgaggttt
     3001 ccgacgaagt tgatcaccgg catcatcagg ctcgagatga actgggcgcg gaagccggcg
     3061 tcgaacagct cgtcgttctt cgcgcggaag cggtcttcga tctgcttgcg gcggccgaac
     3121 actttgacga gcgcgtgccc ggtgaatgct tcttcgatct gaccgttgag cacgccgacg
     3181 tgcttccact gcgcgatgaa gcctttctgc gcgcgcttgc cgatctttgc ggcggccatg
     3241 acggagatcg gcacggtgac gagcgcgatc accgacagca gcggcgagat ccacacgagc
     3301 atgacgagga tgccgacgac cgtgagcagc gcgatcaaga tctggctcat cgtctgctgg
     3361 agcgccatcg cgacattgtc gatgtcgttc gtgacgcggc tcaggacttc gccatgcggc
     3421 tggcgatcga agtacgcgag cgggacgcgc atgagctttg cgtcgacgtc ggcgcgcagc
     3481 ttgcgaatcg tgcgctgcac gacgtagttg aggatgtagc tctgcagcca catcagcacc
     3541 gacgcgccga tgtaaagcgc catgacgagg atcagcgtgt gcgcgagcgc gtcgaagtcg
     3601 atgccctcac ccgcgtgcac gttcatgttg ctggcgagcg cagtgttgac gtggccgccg
     3661 tgggcaacga aacccttgaa cacgatgtcg gtcgcgtgcc cgagcacctt cggacctgcg
     3721 accgagagcg agacgctcgc gatcgcaaac gcgaggacga agacgatcag cttgcgctgc
     3781 ggcgcgagca ggccgatcaa gcgccgcatc gagggaccga agttcttcgc cttttgcggc
     3841 gggcctgtca tgcccatcgg accgcgcatc ggcggaccgg cggaacgcgc gctcgagctt
     3901 gcgggtggag gagcgggtcg gttggtgctc atgccgcggc ctcctgctct tcggtctgtg
     3961 atgtgacgat ctcggcgtag gtcgggcagg tcgtgaccag ctggtcatga gtgcctttgc
     4021 cgacgatctt tccgccgtcg agcacgacga tctgatgtgc gtggcgaatg ctcgcgacgc
     4081 gctgggcgac gacgatgacc gcagtcttcg cgagccgtg
//
LOCUS       DASUFX010000104         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_17128,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000104 DASUFX010000000
VERSION     DASUFX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17128"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="VFV99_04025"
     CDS             complement(<1..1512)
                     /locus_tag="VFV99_04025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058500.1"
                     /translation="MGERLGRYSVLKHLASGGMADVLLARTDGIEGFERHVVLKRIRS
                     EHAKDQRFIQMFLDEARVAAGLHHQNIVQVYDIGEDKGEYFFAMEYIHGEDLRKLLSA
                     VSKKRTHMPLGYVCAILSAAAAGLHYAHERRDNKGKPQNIVHRDVSPSNILIGYDGAV
                     KVVDFGIAKAAMRQVETRSGSLKGKVSYMSPEQCKGEDIDRRSDVFALGVLLYELATT
                     TRLFKGDNDYLVMDAIVNTKVPLPRVRRPDLPNEMASIIMRALSRDPNRRYQTADELR
                     LALDHYAIKNNLTASTSALASYMHKMFGARPEPWLESEASTPNIDVDAPTQAAHHSWT
                     EMARDDAAGFETDDTAATPGTPSRTSSKPGTDSKRVSRRSGVQNAAAVADTIAPPIST
                     RETRTSMKMGWELRRFVGAPEHRNKVLTITVALIALVGIGIWRVAARDDSAPAKAPAP
                     AVAAAKPQPTVEPILTPAPAPVVEQPAAPEIPTVRVAGAEPIPAPAPAVPPRAP"
     gene            1596..>2286
                     /locus_tag="VFV99_04030"
     CDS             1596..>2286
                     /locus_tag="VFV99_04030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058501.1"
                     /translation="MSGAFGNWQLDTLVAVGGLGEVWRARRDGTVAALKRLHTHLARN
                     EEATRQFSVEQKLATTLPRHTNVVHGFESGDVDGRPYVVLELLEGEDLRRMVVPPATK
                     QDATPAQVVIPHARVVQLVAAACDGVAHLHAAGFVHGDVCPGNLMVTTGTSGDRVVVI
                     DLGVARAIGEAGAVRGTHAYMAPEQVRGEAWTRATDVFALGVVLWELVAGERLFHRGP
                     PWLSMAAVVEAE"
BASE COUNT          358 a          739 c          775 g          414 t
ORIGIN      
        1 cggcgctcgt ggaggcacgg ccggcgctgg agccggaatc ggctcggcac ctgccacgcg
       61 cacggtcgga atctcgggcg cggccggttg ctcgacgacc ggcgcgggtg ccggcgtcag
      121 aatcggctcg accgtcggct gcggcttagc cgctgcgacc gctggcgcgg gcgctttggc
      181 aggcgcgctg tcatcgcgtg cagcgacgcg ccagatgccg atgccgacga gcgcgatcag
      241 cgcgaccgtg atcgtcagga ccttgttgcg gtgctcgggc gccccgacga agcgccgcag
      301 ctcccagccc atcttcatgc tcgtgcgcgt ctcgcgcgtc gagatcggcg gcgcgatcgt
      361 gtcggcaacc gccgctgcgt tctggacgcc gctgcgccgc gacacgcgct tgctgtcggt
      421 tccgggcttg ctgctcgttc tgctcggcgt tcctggtgtg gccgcggtgt cgtcggtctc
      481 gaagcccgcg gcgtcgtcac gcgccatctc ggtccagctg tgatgcgcgg cttgcgtcgg
      541 cgcatcgacg tcgatgttcg gcgtgctcgc ttcgctctcg agccacggtt cggggcgcgc
      601 accgaacatc ttatgcatgt agctcgcgag cgccgaggtc gatgcggtga ggttgttctt
      661 gatcgcgtag tgatcgagcg cgaggcgcag ctcgtcggcg gtttgatagc ggcgattcgg
      721 atcgcggctc agcgcgcgca tgatgatcga tgccatctcg ttcggcagat cgggacggcg
      781 cacgcgcggc agcggcacct tcgtgttcac gatcgcgtcc atcacgaggt agtcgttgtc
      841 gcccttgaac agacgcgtcg tggtcgcgag ctcgtagagc aacacaccga gcgcaaagac
      901 gtcggagcgg cgatcgatat cctcgccctt gcactgctcg ggcgacatat acgagacctt
      961 gcccttgagg ctgcccgagc gcgtctcgac ctggcgcatc gccgctttcg cgatgccgaa
     1021 gtcgacgacc ttcacggctc cgtcgtagcc aatcaggatg ttcgacggcg acacgtcgcg
     1081 gtggacgatg ttctgtggct tgcctttgtt gtcgcggcgc tcgtgcgcgt agtgcaggcc
     1141 ggccgcggcc gccgacaaga tcgcgcacac gtagccgagc ggcatgtgcg tgcgcttctt
     1201 cgagaccgcg gaaagcagct ttcgcagatc ctcgccgtgg atgtactcca tcgcgaagaa
     1261 gtactcgccc ttgtcttcac cgatgtcgta gacctgcacg atgttctggt gatgcaggcc
     1321 ggcggcaaca cgcgcctcat cgaggaacat ctggatgaag cgctggtcct tcgcgtgctc
     1381 gctgcgaatt cgcttgagca cgacgtggcg ttcgaagccc tcgatgccat cggtgcgggc
     1441 cagcagtacg tccgccatgc cacccgaggc gaggtgcttg agaacgctgt accgccccag
     1501 tcgctcgccc atcgctcatc aatcgtatga accaaagacc atcggatcaa gttcaacgag
     1561 taagcagcac cacgtgctag ctttgagagc cctacatgtc gggagccttc ggtaactggc
     1621 aactcgacac actcgtcgcg gtgggcggcc tcggcgaagt gtggcgcgca cgacgtgacg
     1681 gaacggtcgc agcgctcaag cgattgcaca cgcatctcgc acgcaacgaa gaagcaacga
     1741 ggcaattctc cgtcgagcag aagctcgcaa cgacgctgcc acgccacacg aacgtcgtgc
     1801 acggattcga atccggcgac gtcgatggca ggccctacgt cgtgctcgag ctgctcgaag
     1861 gcgaagatct ccggcgaatg gtcgtgcctc ccgcgacgaa gcaagacgcc actcccgcgc
     1921 aagtcgtgat tccacacgca cgtgtcgtac agctcgttgc cgctgcgtgc gatggcgtcg
     1981 cacatctgca cgccgcggga ttcgttcacg gcgacgtgtg tcccggaaat ctgatggtca
     2041 cgacaggcac gagcggagat cgcgtcgtgg tgatcgatct cggcgtcgcg cgcgcgatcg
     2101 gcgaggccgg tgcagtgcgc ggcacgcacg cgtacatggc gccggaacag gtgcgtggcg
     2161 aagcgtggac gcgcgcgacc gacgtgttcg cgctcggcgt cgtgctgtgg gagctggtcg
     2221 cgggcgagcg gctgttccac cgcgggcccc cgtggctctc gatggccgcg gtcgtcgagg
     2281 ccgagg
//
LOCUS       DASUFX010000105         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_851231,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000105 DASUFX010000000
VERSION     DASUFX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851231"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFV99_04035"
     CDS             complement(<1..889)
                     /locus_tag="VFV99_04035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5058502.1"
                     /translation="MLGHRGDSCRSNACADPLICSPKLPGGYCASTCGTGEACDGTCV
                     DSRDGELCAKSCASDRDCRSDEGYVCDPQWHACLVPNFAAPVVKQCPTKTPPRDTAFG
                     DAALVAMTGDRRIGTSAVLTDDGDVVTRTDARLARDRKGTVFSVMRSSRVIELTTSTD
                     RGATWSTPLAVDDLDDCGERGCDRPLITIGKEIVYVLYAAGDNGLRVRASHDGGKTFT
                     TGAIALGGTNASAVATGDGKLHVVAMTGSALGAYGSAQQVIQYTVSSDGGATFSPPIV
                     VSGRDEKLPYFFATPVLAVD"
     gene            1108..1947
                     /locus_tag="VFV99_04040"
     CDS             1108..1947
                     /locus_tag="VFV99_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058503.1"
                     /translation="MRRLALSLLVLVLWATGAAAQGMPKVGDPMTLTGLGDSKLQWSY
                     NAPNANDAAGRVVVHWFCTPKVAACVDDLARIVTLREAGGVYIIAYINGSQRDAKKLD
                     PIRESEGVGRGTVASGPSVKKLFKDLGIAKGPASIVVDVDGKVKLVSTSGDINELDSR
                     DEVIKGLVDAVKPYTTTNKAPTKAKVGEKMTFSITVNLASWLSYSKKSTPQLTFTAAK
                     EVKCEPQHVTQADKAMTVSVTCTAPKGSYQARGDIRFGYDSPNGATGLGQEGATWKFD
                     VAP"
     gene            complement(2234..2986)
                     /locus_tag="VFV99_04045"
     CDS             complement(2234..2986)
                     /locus_tag="VFV99_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5058504.1"
                     /translation="MTRKVFLVDDEALALKRLTRLLDATGRVEIVGQGTDPIAAVAAI
                     AALPDLDLVFLDISMPELDGFGVAARLPPRVMVVFTTAHHEHALRAFEVNAIDYLLKP
                     IREADLARALDKHDRLRALPAAEAQAQLAAAMARISLAAPRAPERIASRLGDKIQLVD
                     LERITHFVAEDKLTYAHADGKSYVVDASINQLEERHAGFVRIHRATLVRLAAIVELRA
                     SVDGTRVGLADGTELVVARERVKSLKESLGLG"
     gene            complement(2983..>3332)
                     /locus_tag="VFV99_04050"
     CDS             complement(2983..>3332)
                     /locus_tag="VFV99_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058505.1"
                     /translation="GDRLSYAIEVPDELRALEVPAFAIQTLVENSVKYAVSARKQGAH
                     IQVRARRDGERVAIDIADDGPGFGSEIWVPGHGLDGLRSRLEALYGQRARLIAPIAAQ
                     QGAAVRIEIEAPS"
BASE COUNT          577 a         1075 c         1153 g          527 t
ORIGIN      
        1 tgtcgacggc gaggacgggt gtcgcgaaga agtacggcaa cttctcgtcg cggccgctca
       61 cgacgatcgg cgggctgaat gtggcgccgc catcgctcga cacggtgtat tggatcacct
      121 gttgcgcgga gccatacgcg ccgagcgcgc tgcccgtcat cgcgacgacg tgcagcttgc
      181 cgtcgccggt cgcgacagcg ctggcattcg tgccgccgag cgcgatcgct ccggtcgtga
      241 atgtcttgcc gccatcgtga gatgcgcgca cgcgcaggcc gttgtcaccc gcggcgtaga
      301 ggacgtacac gatctccttg ccgatcgtga tcaacggccg atcgcagccg cgctcgccgc
      361 agtcgtcgag atcgtcgacg gcgagcggcg tgctccatgt cgcgccgcga tcggtcgacg
      421 tcgtcagctc gatcacgcgc gagctacgca tcaccgaaaa caccgtaccc ttgcgatcgc
      481 gtgcgagccg cgcatcggtg cgcgtgacga catcgccatc gtcggtgagc acggcactcg
      541 tgccgatgcg gcgatcgccc gtcatcgcga ccagcgcggc atcgccaaac gcggtgtctc
      601 gcggcggcgt cttggtcggg cactgcttga cgaccggcgc cgcgaagttc ggcaccaggc
      661 acgcgtgcca ctgcggatcg cacacgtagc cttcgtcgct gcggcagtca cggtcactcg
      721 cgcagctctt cgcgcacagc tcgccgtcgc gcgagtcgac gcaggtgcca tcgcaggcct
      781 ccccggtgcc gcaggtcgac gcgcaatagc cacccggcag ctttggcgag caaatcagtg
      841 ggtcggcaca cgcattcgat cggcagctat cgccacggtg accgagcact ggggcggaag
      901 gcgcgtcggg cgcggcgtcg tccagccccg cgtcgatggg agctggaggg gtaggtacgg
      961 ccaactgcct ttgcggactg ggcgaagagc aggctgcggc gatgaataag acgacgattc
     1021 gcaacgtcgc aaggaacgca cgagacccgg gcgtgtgcaa ccgcaacaaa cgcgcccgtt
     1081 ttccctcgtc gcgatataaa aacgcccatg cggagattag ctctaagcct gctcgtgctt
     1141 gttctgtggg caacgggggc ggcggcgcag gggatgccca aggtcggcga cccgatgacg
     1201 ctgaccggcc tcggtgattc gaagctgcag tggtcgtaca acgcgccgaa tgcgaacgac
     1261 gcagcgggcc gcgtcgtcgt gcactggttc tgcacgccca aggtggctgc gtgcgtcgac
     1321 gatctcgcgc gtatcgtgac gctgcgcgaa gccggcggcg tctacattat tgcgtacatc
     1381 aacggcagcc agcgcgacgc gaagaagctc gatccgattc gcgagagcga gggcgtcggt
     1441 cgcggcacgg tcgcgtcggg gccgagcgtg aagaagctgt tcaaggacct cggcatcgcc
     1501 aagggtccgg cgtcgatcgt tgtcgatgtc gacggcaagg tgaagctcgt gagcacgagc
     1561 ggcgacatca acgagctcga ctcgcgcgac gaggtcatca agggcctcgt cgacgcggtg
     1621 aagccctaca cgacgacgaa caaggcgccg acgaaggcga aggtcggcga gaagatgacg
     1681 ttctcgatca ccgtgaacct cgcgagctgg ctctcgtaca gcaaaaagtc aacgccacag
     1741 ctcacgttca cggccgcgaa ggaagtgaag tgcgagccgc aacacgtcac gcaggccgac
     1801 aaggcgatga cggtgagcgt gacgtgcacg gcgcccaagg gaagctacca ggcgcgcggc
     1861 gacatccggt tcggctacga cagcccgaac ggagcgaccg gcctcggcca agagggcgcg
     1921 acctggaagt tcgacgtcgc gccgtaagag tgcgtccggg caggcgtcga tggagcgcgt
     1981 ctgcggcttc gccgctccca gaggaagcca aggcgccaag acgccgagaa cgccagaaga
     2041 aaggtttcgg gatcgcgttc tgctcgcacg accgcgcctg cagatcgcgt cgaagacctg
     2101 ccttctgatg gcgtccttgg cgggcctcgc ttggcgttct tggcgttaga tctggaacac
     2161 gttgtgcggc gccgaaggcg ccagtcggta cctaacgacg gcggtgcggc atcgtgcgtc
     2221 tcggattcga ggcctagccc aggccgagcg attccttcag cgacttgacg cgctcgcgcg
     2281 cgacgacgag ctcggttcca tcggcaagcc cgacgcgcgt tccgtcgacg ctcgctcgca
     2341 gctccacgat cgcggcaagt cgaaccagcg tggcgcgatg gatgcgcacg aagccggcgt
     2401 gacgctcctc gagctggttg atcgatgcat cgacgacgta gctcttgccg tcggcgtgtg
     2461 cgtacgtgag cttgtcctcg gcgacgaagt gcgtgatccg ctcgaggtcg acgagctgga
     2521 tcttgtcgcc cagccgagac gcgatgcgtt cgggagcgcg cggtgcggcg agcgagatcc
     2581 gtgccatcgc ggcggcgagc tgagcttgcg cttcggcggc gggcagtgcg cgcagccgat
     2641 cgtgcttgtc gagcgcgcga gcgagatcgg cctcgcggat cggcttcagc aagtaatcga
     2701 tcgcgttgac ctcgaacgcg cgcaacgcgt gctcgtgatg cgcggtcgtg aacacgacca
     2761 tcacgcgcgg cggcaggcgc gcggcaacgc cgaagccgtc gagctcgggc atcgagatgt
     2821 cgaggaacac gagatcgagg tcggggagcg cagcgatcgc ggcgaccgcg gcgatggggt
     2881 cggtcccttg gcccacgatc tcgacacggc ctgtcgcatc gaggagccga gtgaggcgct
     2941 tgagtgcgag cgcttcgtcg tcgacgagaa acaccttgcg ggtcacgacg gcgcctcgat
     3001 ctcgatgcgt acggctgcac cttgttgcgc cgcgatcggt gcgatcaacc gcgcacgctg
     3061 gccgtacagc gcctcgagtc ggctgcgcag cccgtcgagg ccgtgacccg gtacccagat
     3121 ctcgctaccg aaacctggac cgtcgtcggc gatgtcgatc gcgactcgct cgccgtcgcg
     3181 gcgcgcacgt acctggatgt gcgcaccttg cttgcgcgct gacaccgcgt acttgacgct
     3241 gttctcgacg agcgtctgga tcgcgaacgc cggcacctcg agtgcgcgca gctcgtcggg
     3301 gacctcgatc gcgtagctca gccgatcgcc ga
//
LOCUS       DASUFX010000106         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_434085,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000106 DASUFX010000000
VERSION     DASUFX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434085"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFV99_04055"
     CDS             <1..493
                     /locus_tag="VFV99_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058506.1"
                     /translation="DRALRDVASAVTDELARIADAAALGAPTTRGPVADGLIRRLRHG
                     RLDTLEAGFTRWEERRHSGAVRAPIDEWREWVALRTAYDAAVTAGGLELRRLAFPHAY
                     SKGTSMAVWLWNTRNEYALSHAISKWLLGEALTVGDTEAIELCSRNVKLTVPTRTGEV
                     RGN"
     gene            complement(494..1660)
                     /locus_tag="VFV99_04060"
     CDS             complement(494..1660)
                     /locus_tag="VFV99_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5058507.1"
                     /translation="MKIIRVLVVFAIVCAAAGGFADRDDAPFSPVVYPPQRLPLIFSH
                     AKHAARGTTCVQCHPAAPTSRSTLDNLIPTEAVCRGCHAIDRNDPQKAATPVAACTGC
                     HPGFTPGQPVERIYVMPSPLKFDHSAHAKSSCESCHGDLRNIDLATTRQLPTMASCLS
                     CHKDGSDDRRCTTCHFAQVGGLMETKFSHGELAPQHTGLGDDHGPQFAADHKQQARRA
                     DATCNACHDRSECVECHQGVVKPADFHQANYIALHSIEAKRGRPDCSACHRAQTFCVG
                     CHERSGIGTRGETDFNSRDPLQQFHPPGWASQGAGPNLHAPVARRSINTCASCHREDD
                     CLTCHSADATGLRISPHPAGWRGSSRCRMLDRGNRRMCLRCHITQDELGCDWSK"
     gene            complement(1657..3249)
                     /locus_tag="VFV99_04065"
     CDS             complement(1657..3249)
                     /locus_tag="VFV99_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058508.1"
                     /translation="MLTVATPAHAYEFWFRARTYGQAYQLRDYRLVGPDLFLGRRRVT
                     QMLILRIYDIGDLEATRRLSRLPPGGPRITWQSYLRIDHDFGDFSSGRVLLPGPIRRD
                     ALDVIPELGESVAGLDLMYGYLEVSGLANDRLTLQLGRILTEDGWGTSAFDGGATRVD
                     IPGTPVTLTATGGLHVRASSPLGVAAYELDGTSGAGCTEYVEGPTPGTGSWQLIDRQR
                     TITNRKLASDYEYCPQRDVRQPTIGATLAMTRVHGVSAEVGYRRTWSETVGIIGSVDR
                     FDFPDRGLYPNEFGQAPESGVDENRVYARAHVDVQAGPYAVTPFASARYSILHGLFDR
                     GDLGLRMQRGAHAIEPAIEYYFPTFDGDSIFNAFSIEPTTDMRLAYQYAPRTAWQASA
                     SAWLRKYQNADELSPYAGGVDARLEHMFGGAWRGRVDALYDDGWGGRRVGGAVEGAWK
                     RSQSLFLRGRFVMLDVKEDETSASALGPRNFVTTSAVTSLTYRIGDQAAIHAIAETDY
                     DATNELQFRMFGVLDLNFSPEP"
     gene            complement(3313..>3586)
                     /locus_tag="VFV99_04070"
     CDS             complement(3313..>3586)
                     /locus_tag="VFV99_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058509.1"
                     /translation="PGEAARIEQLSAGTTQTLCSGMSVSAAELEGAVTLTVSGGTATL
                     GIGPLGNVITKVSCAVPTTDRGAWGIAAAGANARIDVGAVTVGRNR"
BASE COUNT          589 a         1188 c         1248 g          561 t
ORIGIN      
        1 cgatcgcgcg cttcgcgacg tcgcgagtgc ggtcaccgac gagctcgcac ggattgccga
       61 tgccgcggca ctcggcgcgc cgacgacgcg tggtccggtc gccgacggac tcattcgccg
      121 gctgcggcac ggccgcctcg acacgctcga agccggattc acgcgctggg aagagcgccg
      181 tcactccggc gccgtgcgtg caccgatcga cgagtggcgc gagtgggtcg cgctgcgcac
      241 ggcatacgac gcggcagtca ccgcaggtgg cctcgagctg cgccgcctcg cgtttccgca
      301 tgcgtactcc aagggcacga gcatggccgt ctggctgtgg aacacgcgca acgagtacgc
      361 actctcgcac gcgatctcga agtggctgct cggcgaagcg cttaccgtcg gtgataccga
      421 agcgatcgag ctgtgctcgc gcaacgtcaa gctgacggtg ccgacgcgca ccggcgaagt
      481 ccgcggcaac tagctacttc gaccaatcgc agccgagctc gtcctgcgtg atgtgacagc
      541 gcaggcacat gcggcgattg ccgcgatcga gcatccggca ccgtgagcta ccgcgccagc
      601 cggcgggatg cggcgaaatc cgtaggccgg tcgcgtctgc actgtggcac gtcaggcaat
      661 cgtcctcgcg atgacacgac gcgcacgtgt tgatgctgcg gcgagcaacc ggcgcgtgga
      721 ggttcggccc cgcgccttgc gacgcccagc ccggcgggtg gaactgctgc aatggatcac
      781 ggctgttgaa gtcggtctcg ccgcgcgtgc cgatgccgga ccgctcgtgg cacccgacgc
      841 agaacgtctg ggcgcggtgg catgccgagc agtcgggacg cccgcgcttc gcctcgatcg
      901 agtgcagcgc gatgtagttc gcctggtgaa agtcggctgg cttcacgacg ccctggtgac
      961 actcgacgca ctcggagcgg tcgtggcacg cattgcacgt ggcgtcggcg cggcgcgctt
     1021 gctgcttgtg atcggccgcg aactgcggcc cgtggtcgtc accgaggcca gtgtgctgcg
     1081 gcgcgagctc gccgtgcgag aacttcgtct ccatcaggcc accgacctgc gcgaagtgac
     1141 acgtcgtgca gcggcggtcg tcgctgccgt ccttgtgaca gctcaggcac gacgccatcg
     1201 tcggcagctg gcgcgtggtc gcgagatcga tgttgcgtag atcgccgtga cagctctcgc
     1261 acgacgactt cgcatgggcg ctgtgatcga acttgagggg cgacggcatc acatagatgc
     1321 gctcgaccgg ctgaccaggc gtgaaacccg gatggcagcc ggtacacgca gcgactggcg
     1381 tggcggcctt ctgcggatcg ttacgatcga ttgcgtggca gccgcggcac acggcctcgg
     1441 tcgggatgag gttatcgagc gtcgagcgcg acgttggcgc tgccgggtga cactgcacgc
     1501 aggtcgtgcc gcgtgccgcg tgcttcgcgt gcgagaagat gagcggcaga cgctgcggcg
     1561 ggtagacgac aggcgaaaag ggcgcgtcgt cgcgatcggc gaatccgcct gctgcggcgc
     1621 agacgatcgc gaatacgaca agaacgcgga tgatcttcat ggctcgggtg agaagttgag
     1681 gtcgaggacg ccgaacatgc ggaactggag ctcgttcgtc gcgtcgtagt cggtctcggc
     1741 gatcgcgtgg atggccgctt ggtcgccgat gcgataggtg agcgaggtga cggcgctcgt
     1801 cgtcacgaag ttgcgcggac cgagcgcgga cgcggacgtt tcgtcttcct tgacgtcgag
     1861 catcacgaac cggccgcgca ggaacagtga ctgcgatcgt ttccacgcgc cctcgacggc
     1921 accaccgacg cgccgccctc cccagccgtc gtcataaagc gcgtcgacgc gaccgcgcca
     1981 cgcgccgccg aacatgtgct cgaggcgcgc atcgacacca ccggcatacg ggctgagctc
     2041 gtccgcattc tggtacttgc gcagccacgc gctcgcgctg gcctgccatg ccgtgcgcgg
     2101 cgcgtattga taagcgagtc gcatatcggt ggtcggctcg atcgagaacg cgttgaagat
     2161 cgaatcgcca tcgaaggtcg ggaagtaata ctcgatggcc ggctcgatcg cgtgggcgcc
     2221 gcgctgcatg cgcaagccga ggtcgccgcg atcgaacaag ccgtgaagga tcgagtagcg
     2281 ggcgctcgcg aacggtgtca ccgcgtaggg accggcttgc acgtcgacgt gcgcgcgcgc
     2341 gtagactcga ttctcgtcga cgccggactc gggcgcctga ccaaactcgt tcggatagag
     2401 gccgcggtcg gggaagtcga agcgatcgac cgagccgatg atgccgacgg tctccgacca
     2461 cgtgcgccga tagccgacct ccgccgagac gccgtgcacg cgggtcatgg cgagcgtcgc
     2521 gccgatcgtc ggctggcgca cgtcgcgctg cgggcagtac tcgtagtcgc tcgcgagctt
     2581 gcggttcgtg atcgtgcgct ggcgatcgat cagctgccac gacccggtgc ccggcgtcgg
     2641 tccttcgacg tactcggtgc aaccggcgcc cgacgtgccg tcgagctcgt atgcggcgac
     2701 gccgagcggc gacgatgcgc gcacgtgcaa gccgcccgtt gcggtgagcg tgacgggagt
     2761 ccccggaatg tcgacgcgcg tggcgccgcc gtcaaacgcg ctcgtgcccc aaccgtcttc
     2821 ggtgaggatg cgaccgagct gcagcgtcaa gcgatcgttg gcgagccccg acacctcgag
     2881 gtagccgtac atgagatcga gcccggcgac cgactcaccg agctcgggga tcacgtcgag
     2941 tgcgtcgcga cgaatcggtc ccggtagcag cacgcgaccg ctcgaaaaat caccgaagtc
     3001 gtgatcgata cgcaggtagc tctgccacgt gatgcgcggt ccgccgggcg gtagtcgaga
     3061 cagcctgcgc gttgcttcga gatcgccgat gtcgtagatg cgcagaatca gcatctgcgt
     3121 cacgcggcgg cggccgagga acagatccgg accgacgagc cggtagtcgc gcagctgata
     3181 cgcctgtccg tacgtgcgcg cgcgaaacca gaactcgtac gcgtgcgccg gcgtagccac
     3241 agtcagcatg gcggcaatga cgcacgcccg gcgcagcatc agtcggacgg taacaccgcg
     3301 cgactccgcg agctaccggt tgcggccgac ggtgacggcg ccgacatcga tgcgcgcgtt
     3361 cgcgccggcg gctgcaatgc cccacgcgcc acggtccgtg gtcggcaccg cgcacgacac
     3421 cttcgtgatc acattgccga gcgggccaat gccgagcgtc gccgtgccac cgctgaccgt
     3481 cagcgtgacc gcgccttcga gctccgccgc actcaccgac atgccactgc acagcgtctg
     3541 cgtcgtgccc gcagaaagct gctcgatccg cgcggcctcg ccgggc
//
LOCUS       DASUFX010000107         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_434702,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000107 DASUFX010000000
VERSION     DASUFX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434702"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3036
                     /gene="carB"
                     /locus_tag="VFV99_04075"
     CDS             <1..3036
                     /gene="carB"
                     /locus_tag="VFV99_04075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5058510.1"
                     /translation="IYIEPIHWRAIAEIIAKERPSALLPTMGGQTALNCALDLVCEGV
                     LEKYGVELIAASREAIDMAEDRELFRHAMKDIGLATPRAGIAHNLEEARQVQEEVGFP
                     TIIRPSFTLGGSGGGIAYNRDEFEDIVTRGLDLSPTNEVLIEESVLGWKEFEMEVVRD
                     KNDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQRMRDASIAVLRKIGVDTGG
                     SNVQFAVSPKDGRLLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNEI
                     TGGATPASFEPTIDYVVTKVPRFTFEKFPQAADRLTTQMKSVGEVMAIGRTFQESLQK
                     ALRGLEIGTDGLNPIMKSVETEEDWAKLKHELRMAGSRRLWYVGDAFRHGLAFEEVAE
                     LTAIDPWFLAQIKELVDIEKQVTAMGPEALMPEELRKLKRRGFSDRRIGDLIGVKEKI
                     VRTARHKENIRPVFKRVDSCAAEFASHTAYLYSTYEEFCEANPTDRQKIMILGGGPNR
                     IGQGIEFDYCCVHASFALKDAGYETIMVNCNPETVSTDYDSSDRLYFEPVTLEDVLEI
                     VHKEKPKGVIVQYGGQTPLKLARALEEAQVPIIGTSPDSIDLAEDRERFQQLVTRLKL
                     KQPPNRTARNVEEAVAMAKEVGYPLVVRPSYVLGGRAMEIVHGEDELRDYVDRAVSVS
                     NDSPILLDRFLDLAVEVDVDAICDGKEVVIGGIMEHVEQAGIHSGDSGCSLPPFSLAP
                     DVQKALSDQVRQMAQALQVNGIMNTQFAIQNGVIYVLEVNPRASRTIPFVSKAIGQPL
                     AKIAAKAMAGISFKEQGFTMPGKRPYFSVKESVFPFSKFPDADPILGPEMKSTGEVMG
                     TGRTFGEAYAKAQLASGVVLPRRGLAFISVRDRDKPGVVELGKMLIARGFEIVATGGT
                     AKALKDGGVPCQRVNKVREGRPHVVDMIKNRELSLIVNTTEGKQAIRESHSIRGEAVH
                     YKVTYYTTVAAAKATCDALDHLDNVIVNRLQDLHAETSV"
BASE COUNT          643 a          990 c         1060 g          502 t
ORIGIN      
        1 atttacatcg agccgatcca ctggcgcgcg atcgcagaga tcatcgcgaa ggagcggccg
       61 agcgcgttgc tgccgacgat gggcggccag acggcgctca actgcgcgct cgatctcgtg
      121 tgcgagggag tgctcgagaa atacggcgtc gagctcatcg ccgcgtcgcg cgaagccatc
      181 gacatggccg aggaccggga gctcttccgc catgcgatga aagacatcgg gctcgcgacg
      241 ccgcgcgccg gcatcgcgca caacctcgag gaagcgaggc aggtgcaaga ggaggtcggg
      301 ttcccgacga tcatccgccc gtctttcacg ctggggggca gcggcggcgg catcgcctac
      361 aaccgcgacg agttcgagga catcgtgacc cgcgggctcg acctgtcgcc gacgaacgag
      421 gtgctcatcg aagagtccgt gctcggctgg aaagagttcg agatggaagt cgttcgcgac
      481 aagaacgaca actgcatcat cgtctgctcg atcgagaatc tcgatcccat gggcgtccac
      541 acgggcgact cgatcaccgt ggcgcccgcg cagacgctga ccgacaagga atatcagcgc
      601 atgcgcgacg cttcgatcgc cgtgctgcgc aagatcggcg tcgacacggg cggctcgaac
      661 gtgcagttcg cggtctcacc caaggatggg cggctgctcg tcatcgagat gaatccgcgc
      721 gtctcgcgtt cgtcggcgct tgcctcgaag gcgacggggt tccctatcgc gaaggtcgcg
      781 gcgaagctcg cagtcggcta cacgctcgac gagctcaaga atgagatcac gggcggcgcg
      841 acgcccgcgt cgttcgagcc gacgatcgat tatgtcgtca cgaaagtgcc gcggtttacg
      901 ttcgagaaat tcccgcaagc cgccgaccgg ctgacgacgc agatgaaatc cgtcggcgag
      961 gtcatggcga tcggccgaac gttccaggag tcgctgcaga aagcgctgcg cggcctcgag
     1021 atcggaaccg acggcttgaa tccgatcatg aagtcggtcg agaccgagga agattgggcg
     1081 aagctcaagc acgagctgcg catggccggg agccgccggc tctggtacgt cggcgacgcg
     1141 ttccgtcacg ggttggcgtt cgaggaagtg gccgagctca cggccatcga tccgtggttc
     1201 ctggcgcaga tcaaggagct cgtcgacatc gagaagcagg tcaccgcgat ggggcccgag
     1261 gcgttgatgc ccgaggagct gcgcaagctc aagcgccgcg gtttcagcga ccgccgcatc
     1321 ggtgacctga tcggcgtcaa ggaaaagatc gttaggacgg cgcgccacaa agagaacatc
     1381 aggcccgtct tcaagcgagt cgacagctgc gccgccgagt tcgcgagcca caccgcatac
     1441 ctctactcga cgtacgagga gttctgcgaa gcgaacccca ccgatcgcca aaagatcatg
     1501 atcttgggcg gcggcccgaa ccgaatcggg caggggatcg agttcgacta ctgctgcgta
     1561 cacgcgtcgt tcgcgctgaa ggacgccggg tacgagacca tcatggtcaa ctgcaacccg
     1621 gaaactgtat cgacggacta cgacagctcg gatcggctgt acttcgagcc cgtcacgctc
     1681 gaggacgtgc tcgagatcgt gcacaaggaa aagccgaagg gcgtcatcgt gcagtacggc
     1741 ggccagacgc cgctgaagtt ggcgcgcgcg ttggaagaag cccaagtgcc gatcatcggc
     1801 acgtcgcccg attcgatcga cctcgccgag gatcgcgagc gcttccagca gctcgtcacc
     1861 cggctgaagc tcaagcaacc gccgaatcgc accgcgcgca acgtcgaaga ggcggtcgcg
     1921 atggcgaagg aagttggcta tccgctcgta gtacggccgt cctacgtgct cggcggccgc
     1981 gccatggaga tcgtgcacgg cgaggacgag ctgcgcgact acgttgatcg cgccgtcagt
     2041 gtctcgaacg acagcccgat tctgctcgac cggttcctcg atctcgcggt cgaggtcgac
     2101 gtcgacgcga tttgcgacgg caaggaagtc gtcatcggcg gcatcatgga gcacgtcgag
     2161 caggccggta ttcattccgg cgactccggt tgctcgctac cgccgttcag cctcgctccc
     2221 gacgtgcaaa aagcgctgag cgaccaagtt cggcagatgg cgcaggcgct gcaggtcaac
     2281 gggatcatga acacgcagtt cgcgatccag aacggcgtca tttatgtgct cgaggtgaac
     2341 ccgcgcgcat cgcgaacgat tccgttcgtg tcgaaggcga tcggccaacc gctcgcgaag
     2401 atcgcggcaa aagcgatggc cggcatcagc ttcaaggagc agggtttcac catgccgggc
     2461 aaacggccgt acttctctgt aaaggagtcg gtgttcccgt tcagcaagtt ccccgatgcc
     2521 gatccgatct tggggcccga gatgaagtcg acgggcgagg tcatgggcac cgggcgaacg
     2581 ttcggcgagg cgtacgccaa agcgcagctc gcctcgggcg tcgtgctgcc gcggcgtggg
     2641 ctcgcgttca tcagcgtgcg tgatcgcgac aagcccggcg tcgtcgagct cggcaagatg
     2701 ctcatcgccc gcggcttcga gatcgtcgcc acgggcggca ccgcgaaggc gctgaaggat
     2761 ggcggagtgc cgtgccagcg cgtcaacaag gtgcgcgagg ggcgcccgca tgtcgtcgac
     2821 atgatcaaga accgcgagct ctcgctgatc gtcaacacga ccgagggtaa gcaagcgatt
     2881 cgcgagtcac actcgattcg cggcgaggcc gtgcactaca aggtcactta ctacacgacg
     2941 gtggccgccg ccaaggcgac ttgcgacgcg ctcgaccatc tcgataatgt catcgtgaat
     3001 cggctccaag acttgcacgc ggagacctcg gtttgagcaa ggtgcccctg acggcgcgcg
     3061 gcgccgccca actcaaagag gagctgcgtc ggctcaagct cgaagagcgg ccgaaggtca
     3121 cggccgcgat cgcgacggcg cgcgcgcacg gcgatctgaa ggagaacgcc gagtaccacg
     3181 cggccaagga gcagc
//
LOCUS       DASUFX010000108        23349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_712417,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000108 DASUFX010000000
VERSION     DASUFX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23349
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712417"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFV99_04080"
     CDS             <1..441
                     /locus_tag="VFV99_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX5058511.1"
                     /translation="SIFAEASELGSDLLHLRKVVLPARSVLTDLSTRKSPFVSEATQP
                     YLGNMVGTIERVLQDVLVDRDILSNSLNNYMSLVAHKTNQVMSKLTVVSIIFLPLTFL
                     CGVYGMNFDIIPELHLEHGYLMFWGLAVVLVGGLLWLMRRNKLL"
     gene            complement(438..1196)
                     /locus_tag="VFV99_04085"
     CDS             complement(438..1196)
                     /locus_tag="VFV99_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5058512.1"
                     /translation="MLKQSLAALVLASSLTACGDRTPQVIAVPTPAADVDKPGQMMIN
                     GQATLEVSPDCADLTITLAVDHIKPGTAVKQLDAKKQALIAALNKIGVETGDVKLSNL
                     QLDPMYEPNPEGWATLKVRTYRAQITVTATTRDFSKIADIMDSAASAGATSVSNQFRR
                     SDLPALKKKVRDMALAAAKDKAKQTADALGIKLGRITSVVENQGGMMWHATYFPQVAN
                     SMEVRNTSDAAVLGGSLQPLTLDVTIGYELGKQS"
     gene            complement(1272..1691)
                     /locus_tag="VFV99_04090"
     CDS             complement(1272..1691)
                     /locus_tag="VFV99_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058513.1"
                     /translation="MKGLVLCLALAACATNPRERPPEVRAVTQAPKAHLPTRGPVHTT
                     PRELFVDFTRPDADGVVLLDKYRDGATFTATIKTVGAEEDGTPVVWVDVDGENLLTLD
                     FADPAPGNLRAGARLTVTCRIGGASGALMMVTRCVSS"
     gene            complement(1821..2291)
                     /locus_tag="VFV99_04095"
     CDS             complement(1821..2291)
                     /locus_tag="VFV99_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058514.1"
                     /translation="MKKWIALGAVIAAGAIVAAIVLTRHHEPAAPAADQVVEDFRATE
                     RQAIAAFNAALRQQRANEIDEIELALAIERDVLEPWRAMRARVAAAPVPANRTELYTV
                     MRRYIDERQVAWQAYADALRTHSEADARPFYDAYHQKNAAAQDDARVLGGLLRR"
     gene            2380..3717
                     /locus_tag="VFV99_04100"
     CDS             2380..3717
                     /locus_tag="VFV99_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX5058515.1"
                     /translation="MTYRPVGEVPEAIHTIARPLHGEQDLDRLVELVAGKRIVMIGEA
                     SHGTHEFYDFRAALSRRLIQHHGFAAVTIEGDWPDALRADRFVREQVTDDRDASTALE
                     AFERFPRWMWRNEDVVDFITWLHGHNSQRDEHLRVGFYGLDLYSLHASMRAVIEYLDD
                     NDHEAAQRARQRYACFDHVQGDPQQYGLEAHLGIGPKCETEVVAQLVDLQLRKLARSG
                     RSPAGDAWFHAVQQAHVVRNAEQYYRMMFAGRSASWNLRDTHMADTLDLLASHLGDGA
                     PAKLIVWAHNSHVGDARATEMSEDGQITLGQIARQRHPGDVALIGFTTHTGTVIAARD
                     WEEPAERMRVRPSLPGSWEELLHESGLPRFYMTAGDMRRVIGEGAERLQRAIGVVYRP
                     VTERRSHYLHARLADELDVVIHIDSTRGVEPLDPIEEAHTPMNELPETFPSGI"
     gene            3720..4091
                     /locus_tag="VFV99_04105"
     CDS             3720..4091
                     /locus_tag="VFV99_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058516.1"
                     /translation="MAHRSKKKHLKHLHEHEPATRKTRPQIVKERAPAAAVAKKGGNG
                     KRANGTAKGPATKPAPKPAPKPAPTKAPEPKAAAPKKGIVRRLAAKARNRITARPRRV
                     VAKMKSRVKSRVQSLLDRIED"
     gene            complement(4127..5416)
                     /locus_tag="VFV99_04110"
     CDS             complement(4127..5416)
                     /locus_tag="VFV99_04110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5058517.1"
                     /translation="MRRILATSGSWGVTEIPWPDVPPPDALNAIQLYILGTSADGPSE
                     DLIGFLRARGPSTPLVVIGRNPARMAVPSLWLPTAPPPALLVAIVNQFLGGEGEGDGS
                     GSKAPWRRKGDMILGSSQHVRDLLHSLDHLAPAQTPVCITGESGVGKELVARALHYSS
                     PRAPQPFIAINCGAVPETLFESELFGYQKGAFTGAVATHIGAFEAADKGTLFLDEIGE
                     MPMAMQVKLLRVLQTYAVQRVGATEAKQVNFRLVTATNRDLAAEVKVGRFREDLFYRI
                     HVYPLHIAPLRERPEDILPIVTHHLSMIAEREKRPHIRVSNGALEKLLAYAWPGNVRE
                     LINTLERAALLAGADQIEAEHVMLPTGTANEAAASLLPYREAKAKFEHEYYSQLMRTA
                     DGNVSLAAKLGQKTRKEIYDALKRLGLDAMAFRSDGD"
     gene            5734..7155
                     /locus_tag="VFV99_04115"
     CDS             5734..7155
                     /locus_tag="VFV99_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058518.1"
                     /translation="MSKTLVWFMAAGLAGCATDPNLESTDEFLSRHNGFIPNGVQIPN
                     ENGHSTTISSQGRIDLSNEFFQDLGINQRRCISCHLPTAQWTVTPEQMQFTFEVTRGG
                     VRDDGFGLGAAFRLNDGANSPNAAVGTLEERRAAYSMLLNKGLIRVGIGIPMGAEFSL
                     VTADDPYGFAHAGELSLFRRPLPATNLKFLSTVMWDGRETFKDEMGEFKSIHFDLSDQ
                     ANGATQGHAQSPVPLSDAQREAIVAFETSLATAQVQDNKAGSLTSGGATGGPAAIFGE
                     EFHIGINDNFGDPVTGAPFTPIVFDLYDKWQGSSKKDRAQIARGQKLFNTRTIHLSGV
                     NGLNVDGLNLGDFDGTCTTCHNTPNAGDHSVVAPLNIGLVAESERTPDMPLYVLQCNA
                     GPHAGQVYRVTDPGRALISGKCADIGKFKGPILRALSGRAPYFHNGSAATLGDAVDFY
                     DRRFNMHLTKNEKADLVAFLGAL"
     gene            7228..8652
                     /locus_tag="VFV99_04120"
     CDS             7228..8652
                     /locus_tag="VFV99_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058519.1"
                     /translation="MRTTLGIIVFALLVGCGGSKPKSTPPPPPGGGGGDTAENTDKTD
                     AVAEAPKPDKPAASGNKALYDRLGGLPAITAVVEEFVTRTTTDPRIKDRFFNTDAANL
                     KKQLVDFVCMATGGPCKYTGRDMVTAHGGMELVDDEFTALVENLVGALDKFKVPEKEK
                     GELLGALGPLKPQIVTPADKLKPIDQKKLDAVTKLAATLKDKEAQELLGLAVQAGKRG
                     QRNYAEQIFSRVEMKVGPKAVASVATTFRAGAPTRVTTATKTLKDEGAQPQVGAVEAE
                     PPPAAKAKVGGLSGNLKIDGKTPSGLGVVMLTPVKGGGKKRVAKTRIIEQRGKEFAPH
                     VMAIPTGSTVSFPNYDQIYHNVFSLSKSKSFDLGMYKNGETREMKFDKPGIIRLGCNL
                     HAAMSAYLIVVAAPHYVVVDKDGKFNFKALAPGKYKVQVWNESAGDPMDAEVEIKEGD
                     NSKDFDLKASPAGVSPDKFGTSRG"
     gene            complement(8718..10517)
                     /locus_tag="VFV99_04125"
     CDS             complement(8718..10517)
                     /locus_tag="VFV99_04125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058520.1"
                     /translation="MGAPSVAVKSGDTLEGRYKIIKTVGEGGMGTVYLAEHMLIKRKV
                     AIKILRSELAADSAVVERFMNEARAAGAIGHPHIVESTDMGFTRDEVPFIVFEYLEGT
                     LLTDEIYRVRGMPPRRALRIATQIASALHAAHTAGIIHRDLKSDNVFLTDKEDASDHV
                     KVLDFGISRFLEAEVGNDDSSNRHLVMGTPEFMAPEQILTPEKVDKRADIYALGVVLY
                     EMLTARRPFDFDEQKLTAERRLTETDKLLHKIVTEPVPLINRFDLPPGLEELIKEKLL
                     AKDPNQRFESMKDVQGAFEAFFSVARRDSEPIPVASMSIPDMPQAAVAVAPGPAPKKP
                     SFGLLIAALLIGGAGAALLAVEPKPNEASAASNDADVKALEDVAAKIGSTLDGQAQAT
                     RLRAEGIGSLPMLRAAIETDAATLKDMVKDRDVLFKAQPDEVLEIFQVRDGKASTMLR
                     IPDEASALKPLGENDTRVESDGTRLHMVVGAPVVNQKGINEGVIALSAPIDLSMIQPL
                     LAPHTKAAAIVGADKPILLAGTAAPGSPTTFAIKTSPQTKINGLSLAAAVDTPAQTAA
                     KAASPTFTYARFACWGLAGLLLVAFGVTRARKN"
     gene            complement(10533..11294)
                     /locus_tag="VFV99_04130"
     CDS             complement(10533..11294)
                     /locus_tag="VFV99_04130"
                     /inference="COORDINATES: protein motif:HMM:NF028434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5058521.1"
                     /translation="MAKILVPLPDHDFDVTEVAVPWKLLREAGHDVVFATEAGATPAC
                     DPLLITGVVFGKLGARPEAIAFYREMEQAPEFVSPRRWAECRVDEFDALFLAGGHAPG
                     MRQYLGSDVVQQIVAAFFASPKPIAAICHGVLVAARAKRADGQSVLHGLRTTCLPKYM
                     ERSAFFSTFWRRGRYYRTYPAYVEDEVRAALAAPEHFARGPRNLSKRGTRGDDTHAFV
                     VEDGRYMSGRWPGDAYLLGKVLIRRLAEGTARISA"
     gene            complement(11319..11669)
                     /locus_tag="VFV99_04135"
     CDS             complement(11319..11669)
                     /locus_tag="VFV99_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058522.1"
                     /translation="MDARSRKMPDDDDRDERDRFVTSGYEDERRRTVRDDNDDREERN
                     RFVHIEERIRTFVGALPAADRERFTDERTRMFEDEEPGRLFGGNRTFGSEERTRERHR
                     SHGHSRFPERHRRR"
     gene            11809..11997
                     /locus_tag="VFV99_04140"
     CDS             11809..11997
                     /locus_tag="VFV99_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058523.1"
                     /translation="MQRHGDMPRKQRFKPSRKPKTDQATVSNPQVDDRKEIHPTTHDI
                     EREVPVDQGPRDIEAEQG"
     gene            complement(12055..12639)
                     /locus_tag="VFV99_04145"
     CDS             complement(12055..12639)
                     /locus_tag="VFV99_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5058524.1"
                     /translation="MQRDPHFRIVVALDLSEHAEIVLEHALDQAARHHAPDIHVIHVV
                     ARDNEARIEDAKRRLGALVMDTLQSFDRGPEWRVRLHIRAGKPSDEVIGLAEDIEAQL
                     IVCGRFGAHHSWRKLGSVAHRILENANCPVFAVHMIDRGVEAPRQCPKCAAVRADTDG
                     EVWFCAEHSASNRESLATVDLPMHADYLGGGPMW"
     gene            complement(12701..15154)
                     /locus_tag="VFV99_04150"
     CDS             complement(12701..15154)
                     /locus_tag="VFV99_04150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5058525.1"
                     /translation="MTACSSRSMPPPAPPIVVAPKVDAPAKAPAEPAPPADRPKRLPH
                     TFEPTSYAARLHVDPAQPEFSGTIEITGALSEPSQLIWLNAEDLRITSVTASRDGKQV
                     PLTVRYALSYGRRNEASAPELALRSEEPLAAGTWTLTLTYTGTWNTRHAVGGFRETAQ
                     GLRLASNETNPKQDAYVFTQFEPDYARGVFPCFDEPDRKVPWQLTLDIPDGLVAASNS
                     AIESETRSGGPYSTKTVRFARTLPLPSYLIAFAVGPFDIVDAGTSKHGTPIRILALRG
                     EGKNVSAAATATPALLDKVEAWLDLTFPYPKLDLVAVPSTENWWLAMENAGLITFNTN
                     ALRDRWEEVVLHEIVHHWFGDFVTPAWWDDFWLNESFAYWLGGELSDSYYWTGDPGEV
                     PIISAVTDPRAHHGGPYASVFKGAQLLALLDATVGTEPLKRALRGYLTAHAHGTVRND
                     DLRDALKPSLGTDFAGIFERYLSSPTAELDVELNCEGKSKRIEVKGFDTVGLLCVAYD
                     HDGARADRCTKLAWGSATLELGTKRCPRWVLPKTLHHLTWTAQELEALRDRAWPMLTK
                     NERMTVISVALANGTRGALRLSFIEKAAELDDPNLTKWITTYLYGLGPQIPASLRPQY
                     DAWITKHFASRARAIGIAPTGVSIEKRRDAYELLALVLDAGDPTLRADALKLLPKLDD
                     LDAASRVLVLRAAILDKPAFADELVSQLPKVNRTRMTDIAFALERTPDLLGLLQRHVA
                     EIKLLQDVEKVRLLNQVCDGTKRADVEKLGRQIDPKWDQFSIRNFEWCVTERKALEPA
                     LRAFLTGAPVAPKASQANH"
     gene            complement(15177..16121)
                     /locus_tag="VFV99_04155"
     CDS             complement(15177..16121)
                     /locus_tag="VFV99_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5058526.1"
                     /translation="MCLVLAACGQDTLATKPDARPDDTHPDALGVCGIRTGMRGKTDR
                     TIRAAALDRTYIVYLPPNVDPKERIPLVFVHHGFTMSGQAMFDITGYNELADAEHIAV
                     AYPDGQGGPDTLNAPWNVGIDVCPAQGGGVPPNAPGDDFTLLDAIKADISADQCLDLD
                     HIYVTAFSMGGYFSHHTGCMRDDIRAVAPHSGGTHPFDSCTTQHKPVIIFHGTSDTIV
                     PNSCDDPSVPQPQGLTPSATEWARKNGCAGTVTRKDVNGGSCIYYDGCPADGQVALCT
                     FTSMGHCWAGGKSGSPFACPTFESATQLEWAFFKQYAW"
     gene            16233..17255
                     /locus_tag="VFV99_04160"
     CDS             16233..17255
                     /locus_tag="VFV99_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5058527.1"
                     /translation="MSSPRLRRTSRLADAKVALSLARAAFDKDRPLIANLIITRRCNL
                     SCGYCYEYDKVSPPVPLETLKERIDHIARLRAVFVTMTGGESLLNPDADKAVAYIREK
                     GMVPFLNTNAYLLTKQWIDRLNAAGLYGMQISIDNVNPNDVSKKSLKTILPKLKLLAK
                     YARFRVRINCVLGSSPPAEAIEVARTVIGLGFDMNTSLVRAEDGTLLPVSEETRAAYE
                     TIRGLGKRAPFFLDDDYTKPLMDNGESEWKCRAGARTFHVDEFGMVHLCAPRVGSPGK
                     SIAEYTALDIRYWFNAPKSCASKCPVAYARHASRLDGWRSQNGDLMQLPAPQPQLVQL
                     RLSRAA"
     gene            complement(17256..17468)
                     /locus_tag="VFV99_04165"
     CDS             complement(17256..17468)
                     /locus_tag="VFV99_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058528.1"
                     /translation="MYRLVTCPETAHLELIEYEETPVGKVIAACSRFRPACAVGCPRT
                     CAARMDRRDMHTLGLEFGEDTDVTSR"
     gene            complement(17611..18282)
                     /locus_tag="VFV99_04170"
     CDS             complement(17611..18282)
                     /locus_tag="VFV99_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5058529.1"
                     /translation="MEARTLGQFVADEGILESLRTMIADINARATSEAKKVGKQEKLP
                     VVQCFKCDAKKACCHSVVVARLYEGIVVAAHLKQASRDTPALRDELRTRAEAMEAASP
                     YEWRVPCLFLDDNERCTVYAARPTPCGTLYVYTPPAHCNDPSKPVHAYIPDAEHAVAQ
                     QIEDEFRDKLSLRKKVGRRYIGVLPRMVLVALEAWDRTDFRDYLRQLEWPTDTEADRW
                     RPRAP"
     gene            18289..18870
                     /locus_tag="VFV99_04175"
     CDS             18289..18870
                     /locus_tag="VFV99_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5058530.1"
                     /translation="MLNVVVAGVIKKTATIDAGDAQSGGSVRLAVVADTHSAPHPKTA
                     ERLAELKPDAILHGGDIGDLQVLDELAKVAPVYAVRGNIDTHARDLPDVLTLEVGNVR
                     ILLTHIAVYGPRLRGEIAKLAREEHAKLVVCGHSHVPFIGLDKGIEVFNPGSIGPRRF
                     NLPILLGAIDITPTSLKMRHIEAETGKLWLPPR"
     gene            complement(18884..19585)
                     /locus_tag="VFV99_04180"
     CDS             complement(18884..19585)
                     /locus_tag="VFV99_04180"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HEX5058531.1"
                     /translation="MTSILVPACTSQPADELSDESADGEGGKADSAATFTFLNLIPDT
                     RACSLDSPGDCGNGFFVSRANRSTIQCGRGPAQSQCKVQTIDWSGTAMPASVAQGYED
                     DLRAGKPLLVKGTVEPDPADRGLVVNVTEIWVASAPEWVDGVFALVHDNGIRCVRAPC
                     PSLTEKKLNSSLSAQITGIDFDASGADQKAIDRARDALYGDGLIVVGYRDYDSLGGKT
                     RTANKFFTKAPVPLH"
     gene            complement(19709..20446)
                     /locus_tag="VFV99_04185"
     CDS             complement(19709..20446)
                     /locus_tag="VFV99_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058532.1"
                     /translation="MADRAATRLAIDLGFVGLLIACAITVYAQTRAPDIPDGVCRADS
                     RGPRYDAMLADGRITVAAVFGELQGGPTDHNLWSYRAFASALRDRGFAEDGDRFTLGN
                     VTIDLALVPDDPAQTSAALAGALARHDVVYYNGHSHDGAIDISPPHDYRLIVLDSCWS
                     TQEYAQRLVGPERDVLTNSDRSVTGSVESFVALLDAIRAHEHWPLDEMNALAEARAHK
                     RAPVSRFKDPERYRVDGPCNASASRRD"
     gene            complement(20419..22500)
                     /locus_tag="VFV99_04190"
     CDS             complement(20419..22500)
                     /locus_tag="VFV99_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5058533.1"
                     /translation="MRSLLVLALLASCHAGMTMSADVTISGSVGYAAMDEIPVAGGEL
                     RLVAPQVAVFEGVKQEVIGFPLKHTAITANVGGMMAVYNVEQIFENPFDEPIEAVYVF
                     PLGDDGAVSGYEIGIGERTITGEIKTKEDARRIYDEAKSSGHTAGLVEQNKPNIFTQH
                     IANIAPHETIKIRIRYVELLDYDNGAYTMAVPLTVGPRYLPADRQGRNPVSAASMPYV
                     SSERASSTVSFTADIDAGVPIVGVESPSHDILATQPAVTRSHITLGRSDEIPNRDLVI
                     RYKTAGPQTTVGLLTHKVDEDGYFMLAVQPKATYRTGDITPREVMIVIDRSGSMDGQP
                     LAQAKAVASAIIDTLTERDSFNVVAFASGVEAMADNPIRGDGAGKQRGLEYLKVLQSG
                     GGTEMEQGVVRMLTATPGSDRIRVVYFLTDGFVGNDDVVVGAAKRFLGTNRIFTVGIG
                     SAPNRSLLDKLAGAGRGFASYLTLTESAEKLAKNLVFKSAYPYMTDVQVDWGGLAVEE
                     LTPAMVPDVYAGQPLIVTGRYRRPGQAVIKVSATTAGKRVTIPIAVTLPPVNNFEPVA
                     SLWARRKIDVLMDKADEEDVRKPVTELGLRFHLVTEFTSFVAVDRTRIVSNGQVRVVE
                     QPSVVPEGVNPATTVNEAPDPAPSSSRRSTYDDGGGFGGWGGGGRSLGDWWILVFFGI
                     GALWLIVRRLV"
     gene            complement(22633..>23349)
                     /locus_tag="VFV99_04195"
     CDS             complement(22633..>23349)
                     /locus_tag="VFV99_04195"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058534.1"
                     /translation="TNARAAVGALPADPLAAARDAHTRGLVRRALALLDLAIEAKPDD
                     PAAYAEKGRQLFFASFPEHAATLLKRAIALGDQTPSTHGWLGAVLADTPEQADAVPLL
                     QRAVQGELDGGMWWHSWVRLLVRLDRIDEAEAELSRALALPLDARWHGGLLTEKVNVL
                     CARKRPADALEAADAALQLAPGSDYAHFVRGRALGMLGRLDEAELEMDKILHNTPNHA
                     DATRAKQVYAAARARRQAKR"
BASE COUNT         3978 a         7552 c         7708 g         4111 t
ORIGIN      
        1 tcgatcttcg ccgaggcgag cgagctcggc tccgatctct tgcatctgcg caaggtcgtg
       61 ctgcccgcgc gctcggtgct caccgatcta tcgacgcgca aatcgccgtt cgtcagcgaa
      121 gcgacgcagc cgtacctcgg caacatggtc ggcacgatcg agcgcgtgct gcaggacgtg
      181 ctcgtcgatc gcgacatcct gagcaactcg ctcaacaact acatgtcact cgtcgcgcac
      241 aagacgaacc aggtgatgag caagctgacc gtcgtcagca tcatctttct gccactgacg
      301 ttcttgtgcg gcgtctacgg catgaacttc gacatcatcc ccgagctgca cttggaacat
      361 ggttatttga tgttctgggg gctcgcggtt gtcctcgtcg gtggcttgct ctggctaatg
      421 cgtcgcaaca agctgctcta gctctgcttg ccgagctcgt agccgatcgt gacgtccagc
      481 gtgagcggct gcagcgagcc gccgagcacc gccgcgtccg acgtgttgcg cacctccatc
      541 gagttcgcga cctgcgggaa gtacgtcgcg tgccacatca tcccgccctg gttctcgacg
      601 acggacgtga tgcggccgag cttgatgccg agcgcgtcgg ccgtctgctt cgccttgtcc
      661 ttcgcggcgg cgagcgccat gtcacgcact ttcttcttga gcgcggggag atcgctacgc
      721 cggaactgat tcgagaccga cgtcgcgccc gcgctcgcgg cgctgtccat gatgtccgcg
      781 atcttcgaga agtcccgggt cgtcgcggtg acggtgatct gcgcgcggta ggtacgaacc
      841 ttgagcgtcg cccagccttc cgggttcggc tcgtacatcg gatcgagttg caggttcgat
      901 agcttcacgt cgccggtctc gacgccgatc ttgttgagcg cggcgatcaa cgcctgcttc
      961 ttcgcgtcga gctgcttcac ggcagtgccc ggtttgatgt gatcgaccgc gagcgtgatc
     1021 gtgagatcgg cgcagtcggg gctgacctcg agcgttgctt ggccgttgat catcatctga
     1081 ccgggtttgt cgacgtccgc cgccggcgtc ggtacggcga tgacctgcgg cgtgcgatca
     1141 ccgcacgcgg tcagcgagga agcgaggacg agggcggcga gactctgttt cagcatggcg
     1201 agctccttct gccatgaaga cggacgtcac cgcgttcggt atgcctcgcg cggcggtgcc
     1261 ttacacaagc cttacgagga cacgcaccga gtgaccatca tgagcgcgcc gctcgctccc
     1321 ccgatgcggc acgtgacggt gagtcgggca ccggctcgca ggttgccggg cgccggatcc
     1381 gcgaagtcga gcgtcagtag gttctcgccg tcgacgtcga cccacacgac cggtgtgccg
     1441 tcctcttctg cgccgacggt cttgatcgtc gccgtgaacg ttgcaccgtc gcgatacttg
     1501 tcgagcaaca cgacgccatc ggcgtcgggc cgcgtgaagt cgacaaacag ctcgcgcggg
     1561 gtcgtgtgca ccggaccgcg cgtcggtagg tgggctttcg gcgcctgtgt cacggcgcgc
     1621 acttcgggtg gccgctctct tggattcgtc gcgcaggctg caagcgcgag gcacagcacg
     1681 aggcccttca tgcgcgcgtg tagatcaagt cgcgtgccgg tctaagtccg cgacggcgcg
     1741 tgcgtgcgcg ggtctacgga gacccaaaca agccgggaag cgggcccgtg cagcgctcga
     1801 gatcgccggc ttgagcccag ctatcgacgg agcaggcctc cgagcacgcg cgcgtcgtcc
     1861 tgcgccgctg cattcttctg gtgatacgcg tcgtagaacg gacgcgcgtc ggcttcggag
     1921 tgtgtccgca acgcgtcggc gtatgcctgc cacgcgacct gacgttcgtc gatgtaacgg
     1981 cgcatcaccg tgtacagctc cgtgcgattt gcgggaacgg gcgccgccgc gacgcgtgcg
     2041 cgcatcgcac gccacggctc gagtacgtcg cgctcgatcg ccagcgctag ctcgatctca
     2101 tcgatctcgt tcgcgcgctg ttggcggagt gccgcattga atgcggcgat tgcttgccgc
     2161 tcggtcgccc gaaaatcttc gacgacctga tcggcagcgg gcgctgcggg ttcgtgatgg
     2221 cgcgtcagca cgatcgcagc aacgatcgcg ccggccgcga tcaccgcacc gagagcgatc
     2281 cacttcttca ccgcgcgttc atagcactgc gcgccgacgc gcggcgtgcg atgcggtgcg
     2341 cacatttgac tgcagtttta ctgacttcaa ggtttgctca tgacgtatcg ccccgtcggc
     2401 gaggtgcccg aagcgatcca cacgatcgcg cggccactgc acggcgagca ggatctagat
     2461 cggctcgtcg agctcgtcgc gggcaagcgc atcgtgatga tcggcgaggc gagccacggc
     2521 acccacgagt tctatgattt tcgtgccgcg ctctcgcgcc gattgatcca gcatcacggc
     2581 ttcgccgccg tgacgatcga aggcgattgg ccggacgcac tccgtgcgga tcgcttcgtg
     2641 cgcgagcaag tcaccgacga ccgcgacgcg agtaccgcgc tcgaagcgtt cgaacggttt
     2701 ccgcgctgga tgtggcgcaa cgaagatgtc gtggacttca tcacgtggct gcacggccac
     2761 aacagccagc gcgacgagca cctgcgcgtc ggcttctacg gcctcgatct gtattcgctg
     2821 cacgcgtcga tgcgcgcggt gatcgaatat ctcgacgaca acgatcacga agcagctcag
     2881 cgcgcacgcc agcgctacgc gtgcttcgat cacgtgcagg gcgatccgca gcaatacggg
     2941 cttgaggcac acctcggcat cggcccgaag tgcgagaccg aggtggtcgc gcagctcgtc
     3001 gatctgcagc tgcgcaagct cgcacgctcg ggacgctcgc ccgccggcga cgcatggttc
     3061 catgccgtcc agcaagcaca cgtcgtccgc aacgccgagc agtactaccg gatgatgttc
     3121 gcgggccgta gcgcgtcgtg gaacctgcgc gacacgcata tggccgacac gctcgacctg
     3181 ctcgcaagtc atctcggcga cggtgcgcct gcgaagttga tcgtctgggc gcacaactcg
     3241 cacgtcggcg acgctcgcgc gaccgagatg agcgaagacg gccagatcac gctcggccag
     3301 attgcacgcc agcgtcatcc cggcgacgtc gcgctgatcg gcttcacgac gcacacaggc
     3361 accgtgatcg cagcgcgcga ctgggaagag ccggccgagc gcatgcgcgt gcggccgtcg
     3421 ctaccgggca gctgggaaga actgctgcac gaatccggat tgccgcgctt ctatatgacg
     3481 gcgggcgaca tgcggcgggt gatcggcgaa ggcgccgaac gattgcagcg cgcgatcggc
     3541 gtcgtgtatc ggccggtcac cgagcgccgc agccactatc tgcacgcgcg gctcgccgac
     3601 gagctcgatg tcgtcatcca tatcgattcg acccgcggcg tcgagcccct cgatcccatc
     3661 gaggaagccc acacgccgat gaacgagctg cccgagacct tcccctcagg aatctagtta
     3721 tggcccatcg atccaagaag aagcacctca agcacctgca cgagcacgag ccggcgacgc
     3781 ggaagacgcg gccgcagatc gtgaaggaac gcgcacccgc agcggctgtc gcgaagaagg
     3841 gcggcaacgg gaaacgcgcg aacggcaccg cgaagggacc ggcgacaaag ccagcgccga
     3901 agccagcacc aaagcccgca ccaacgaagg cacccgagcc gaaggcagct gcgccgaaga
     3961 aaggcatcgt gcggcgcctc gctgcgaagg cgcggaaccg gatcaccgcg cggccgcgtc
     4021 gcgtggtcgc gaagatgaag tcgcgcgtga agtcgcgcgt tcaatcgctg ctcgaccgca
     4081 tcgaggacta atcggccgcg ccgctggcgt tcacgcctcc gcgcgatcag tcgccgtcgc
     4141 tacgaaatgc catcgcgtcg aggccgaggc gcttgagtgc gtcgtagatc tctttgcgtg
     4201 tcttctggcc gagcttggca gcgagcgaca cgttgccatc ggcggttcgc atcagctgcg
     4261 agtagtactc gtgctcgaac ttcgccttcg cctcgcgata cggcagtagt gacgcggcgg
     4321 cctcgttcgc ggtgccggtc ggcaacatca cgtgctcggc ttcgatctgg tcggcaccgg
     4381 cgaggagcgc cgcgcgttcg agcgtgttga tgagctcgcg gacgttgcct ggccacgcgt
     4441 acgcgagcag cttctcgagc gcgccattcg acacgcggat gtgcggccgc ttctcgcgct
     4501 ccgcgatcat cgataggtga tgcgtcacga tcggaagaat gtcctcggga cgttcgcgca
     4561 gcggcgcgat gtgaagcgga tagacgtgga tgcggtagaa caggtcctcg cggaagcgcc
     4621 cgaccttcac ctcggctgcg agatcacggt ttgtcgcggt cacgaggcgg aagttgacct
     4681 gcttggcttc ggtcgcgccg acgcgttgca cggcataggt ctgcagcacg cgcagcagct
     4741 tgacctgcat cgccatcggc atctcgccga tctcgtcgag gaacagcgtg cccttgtcgg
     4801 ccgcttcgaa cgcaccgatg tgcgttgcaa cggcgccggt gaacgcgccc ttctggtaac
     4861 cgaacagctc cgattcgaac aacgtctcgg ggaccgcgcc gcagttgatc gcgatgaacg
     4921 gttgcggcgc gcgcggcgag ctgtagtgga gtgcacgggc gacgagctcc ttgcccacgc
     4981 cactctcgcc ggtgatgcac accggcgtct gcgccggcgc gagatggtcg agcgagtgca
     5041 gcagatcgcg cacgtgttgg ctcgaaccga ggatcatgtc gcccttgcgc cgccacggcg
     5101 ccttcgaacc gctgccgtcg ccctcaccct caccgccgag gaactgattg acgattgcca
     5161 cgagcaacgc gggaggagga gcggtgggca gccacaagct cggcaccgcc atgcgcgctg
     5221 gattgcgccc gatcacgacg agcggcgtgc tcgggccgcg ggcgcgcaga aagccgatca
     5281 ggtcctcgga tggtccatcc gcgctggtgc cgaggatgta gagctggatt gcgttcagcg
     5341 cgtcgggagg cggaacgtcc ggccaaggga tctcagttac cccccaacta cccgaggtag
     5401 ctaagattct tcgcacctcg gccaacccac tagacccagg tccacataca ccgatatgcc
     5461 cgaaattact cggaggcatc cttaccttat ttaaccccta tggctgtaaa acgatacacc
     5521 gtaatcgagc ctgcaaaact ttcatccgag tttccagtaa ttagccgcag atcggccgat
     5581 gccctaagtt ccgtacaagg tttcagctac ttgcgcggga gtggctggtt tttggtacac
     5641 ggttagtttt gggttaccta aaacccaaag gaaaatcagt aactaacgcc cagggtgtcg
     5701 ttggcgctcg gcctgcagag agaacccccc actatgtcga agacactcgt gtggttcatg
     5761 gcggcgggtc tggctgggtg tgcgacagat ccaaacctgg aatcgacgga cgagttcctg
     5821 tctcgtcaca acgggttcat cccgaacggc gtgcagatcc cgaacgagaa cggtcactcg
     5881 accaccatct cgtctcaggg ccgcatcgac ctgagcaacg agttcttcca ggacctcggc
     5941 atcaaccagc gccgttgcat cagctgccac ctgccgaccg cgcagtggac ggtgacgccg
     6001 gagcagatgc agttcacgtt cgaagttacg cgcggtggcg tgcgtgatga tggcttcggt
     6061 ctcggcgcag cattccgctt gaacgacggc gcgaactcgc cgaacgcggc ggtcggcacg
     6121 ctcgaggagc gccgcgctgc gtacagcatg ctcttgaaca agggcctcat ccgcgtcggc
     6181 atcggcatcc cgatgggcgc ggagttctcg ctcgtcaccg cggatgaccc gtacggcttc
     6241 gcgcacgccg gcgagctctc gctgttccgc cgcccgcttc cggcgacgaa cctcaagttc
     6301 ctcagcacgg tcatgtggga cggtcgcgag acgttcaagg acgagatggg cgagtttaag
     6361 tcgatccact tcgacctctc ggatcaggcg aacggcgcga cgcagggcca cgcccagtcg
     6421 ccggtcccgc tcagcgacgc gcagcgagaa gcgattgtcg cgttcgagac ttcgctggcg
     6481 acggcgcagg tccaggacaa caaggcgggc agcttgacca gcggcggcgc cacgggtggt
     6541 ccggcggcga tcttcggtga agagttccac atcggcatca acgacaactt cggtgacccg
     6601 gtcaccggcg cgccgttcac gccgatcgtt ttcgacctct acgacaagtg gcagggcagc
     6661 tcgaagaagg accgcgcgca gatcgctcgt ggccagaagc tgttcaacac ccgcacgatt
     6721 cacctctcgg gcgtcaacgg cctcaacgtc gacgggctca acctcggtga cttcgacggt
     6781 acgtgcacca cgtgccacaa cacgccgaac gcgggtgacc actcggtcgt cgcgccactc
     6841 aacatcggcc tcgtcgccga gtcggagcgt acgcctgaca tgccgctgta cgtcctgcag
     6901 tgcaacgcgg gtccgcacgc gggccaggtc tatcgcgtga ccgacccggg ccgcgcgctc
     6961 atcagcggca agtgcgccga catcggtaag ttcaagggcc cgatcctccg cgctctctcg
     7021 ggtcgcgcgc cgtacttcca caacggctcg gcggcgacgc tgggtgacgc ggtggacttc
     7081 tacgaccgcc gcttcaacat gcacctgacg aagaacgaga aggcggacct cgtggccttc
     7141 ctcggcgcgc tctaagcaat ccgctgtcac aaaggaaacg ccctgccatt gcgcagggcg
     7201 tttttatttg gtgataacgt cccgcgcatg aggaccaccc tagggatcat tgtgtttgcg
     7261 ttgcttgtcg gctgtggtgg ctcgaagcca aagtcgaccc caccaccgcc accgggaggc
     7321 ggcggaggag acaccgcaga gaatacggac aagaccgacg cggtcgcgga agctccgaag
     7381 ccggacaagc ccgctgcatc gggaaacaag gcgctgtacg accgcctggg cggcctgccg
     7441 gccatcacgg ccgtcgtcga ggagttcgtg actcgcacga cgaccgaccc gcgcatcaag
     7501 gaccggttct tcaacaccga cgcggcgaac ctcaagaagc agctcgtcga cttcgtgtgc
     7561 atggcgacgg gtggtccctg caagtacacc ggccgcgaca tggtgacggc gcacggcggc
     7621 atggagctcg tcgatgacga gttcaccgca ctcgtcgaga acctcgtcgg cgcactcgac
     7681 aagttcaagg tgcccgagaa ggagaagggc gagctgctcg gcgcgctcgg cccgctgaag
     7741 ccgcagatcg tgacgccggc cgacaagctg aagccgatcg accagaagaa gctcgacgcg
     7801 gtgaccaagc tcgccgcaac gctcaaggac aaggaagcgc aggagctgct cggtctcgcc
     7861 gtccaggcgg gcaagcgcgg ccagcgtaac tacgcggagc agatcttcag ccgcgtcgag
     7921 atgaaggtcg gcccgaaggc ggttgcctcg gttgcgacga cgttccgcgc gggcgcgccg
     7981 acgcgcgtca ctaccgcgac gaagacgctc aaggacgaag gcgcgcagcc gcaggtcggc
     8041 gccgtcgagg cagagccgcc gccggccgcg aaggcgaagg tcggcggtct gtcgggtaac
     8101 ctcaagatcg acggcaagac gccatcgggc ctcggcgtcg tcatgctgac gccggtcaag
     8161 ggcggcggca agaagcgcgt cgcgaagacc cgcatcatcg agcagcgcgg caaggagttc
     8221 gcgccccacg tgatggcaat ccccaccggc tcgacggtgt cgttcccgaa ctacgaccag
     8281 atttatcaca acgtgttctc gctctcgaag agcaagtcgt tcgatctcgg catgtacaag
     8341 aacggcgaga cgcgtgagat gaagttcgac aagccgggca tcatccgcct cggttgcaac
     8401 ctgcacgcgg cgatgtcggc gtacctgatc gtcgtcgcgg cgccgcacta cgtcgtcgtc
     8461 gacaaggacg gcaagttcaa cttcaaggcg ctcgcgcccg gcaagtacaa ggtgcaggtc
     8521 tggaacgagt cggccggcga tccgatggac gccgaggtcg agatcaagga aggcgataac
     8581 agcaaggact tcgatctgaa ggcctcgccc gctggcgtct cgcccgacaa gttcggcacg
     8641 tcgcgcggct agtccagcgc gctactccac gtcgccccga gcaacgatct gattcggggg
     8701 cagcagcgtg gagcgcctca gttcttgcga gctcttgtca cgccaaacgc gacgagcaag
     8761 agcccggcaa ggccccagca cgcgaagcgt gcataggtga acgtcggcga cgctgccttc
     8821 gcagcagttt gcgcgggggt atcgacggcc gctgcgagcg agagcccgtt gatcttcgtc
     8881 tgcgggctcg tcttgatcgc aaacgtcgtc ggcgatccgg gcgccgccgt gcccgcgagc
     8941 aggatcggct tgtcggcgcc gacgatcgcc gccgccttgg tatgcggcgc gagcagcggc
     9001 tgaatcatcg agaggtcgat gggcgccgac agtgcgatca cgccctcgtt gatgcccttc
     9061 tgattgacga ccggtgcacc cacgaccatg tgcaagcggg tgccgtcgct ctcgacgcgc
     9121 gtgtcgttct cgccgagcgg cttgagcgca gacgcttcgt cggggatgcg caacatcgtc
     9181 gacgccttgc cgtcgcggac ctggaagatc tcgaggacct cgtcgggctg cgccttgaac
     9241 aagacgtcgc ggtctttgac catgtctttg agcgtcgccg cgtccgtctc gattgcagcg
     9301 cgcagcatcg gcaggctgcc aatgccttcc gcgcgtaggc gtgtggcctg cgcttggccg
     9361 tcgagcgtcg agccgatctt ggcagcgaca tcctcgagcg ctttgacatc ggcgtcgttg
     9421 ctggcggcgg acgcttcgtt cggcttgggc tcgacggcga gcagcgcagc accggcacca
     9481 ccgatgagca gcgcggcgat gagcaggccg aagctcggct tcttcggcgc tgggccgggc
     9541 gcgaccgcga ccgccgcttg cggcatgtcc ggtatcgaca tcgacgcgac cggaatcggc
     9601 tcgctgtcgc gacgcgcgac gctgaagaac gcctcgaatg cgccctgcac gtccttcatc
     9661 gactcgaaac gctggttggg atctttcgcg agcagctttt ccttgatcag ctcctcgagg
     9721 cccggcggca gatcgaagcg attgatcagc ggcaccggct ccgtgacgat cttgtgcagc
     9781 agcttgtcgg tctcggtgag acggcgctcg gcggtgagct tctgctcgtc gaagtcgaac
     9841 ggccggcgcg cggtgagcat ctcgtagagc accacgccga gcgcgtagat gtcggcgcgc
     9901 ttgtcgacct tctccggcgt caggatctgc tcgggcgcca tgaattccgg cgtacccatg
     9961 acgaggtggc ggttcgacga gtcgtcgttg ccgacctccg cctcgaggaa gcgcgagata
    10021 ccgaagtcga gcaccttgac gtgatcgctc gcgtcctctt tgtcggtgag gaagacgttg
    10081 tccgacttga ggtcgcggtg aatgatgccg gctgtgtgcg cggcatgcag tgcggatgca
    10141 atttgtgttg cgatgcggag cgcacggcgc ggcggcatac cccggacgcg ataaatctcg
    10201 tcggtgagca gcgtaccttc gaggtactcg aacacgatga acggcacttc atcgcgcgtg
    10261 aatcccatgt ccgtcgactc gacgatgtgg ggatgaccga tcgcgccggc cgcgcgtgct
    10321 tcgttcatga agcgctctac aaccgcagaa tctgcggcga gctcgctgcg caggatcttg
    10381 atcgcgacct tgcgcttgat cagcatgtgc tcggccagat agaccgtgcc catcccgcct
    10441 tcgccgaccg tcttgatgat cttgtaacgg ccttcgagcg tatcgcctga cttgaccgcg
    10501 acgctcgggg cacccattac agaaccattc tatcaggcgg agatacgtgc tgtgccttcc
    10561 gcaagccgcc tgatgagtac ctttccgagg aggtacgcgt ccccgggcca tcggcccgac
    10621 atgtagcggc cgtcctcgac gacgaacgcg tgcgtgtcgt cacctcgcgt gccacgctta
    10681 gataagttgc gggggccgcg cgcgaagtgc tcgggtgcgg caagtgcagc gcgcacctcg
    10741 tcttcgacat aggccggata ggttcggtaa tagcggccac gtctccagaa cgtcgagaaa
    10801 aacgctgacc gttccatgta cttcgggaga caggtcgtcc gcaggccgtg gagcaccgat
    10861 tggccgtccg cccgcttggc ccgcgcggcg accagcacgc catgacagat ggcagcgatc
    10921 ggtttgggcg acgcgaagaa ggccgcaacg atttgctgca ctacgtcgct gcccaggtac
    10981 tggcgcatgc ctggcgcgtg accgccagcg agaaacagtg catcaaattc gtcgacccgg
    11041 cattccgccc accgccgcgg ggacacgaat tccggcgcct gctccatctc gcgatagaac
    11101 gcgatcgctt cgggccgtgc gccgagcttg ccgaacacga cgccggtgat cagcagcgga
    11161 tcacacgcgg gtgtcgcgcc ggcttcggtc gcaaaaacga cgtcgtggcc ggcctcgcgg
    11221 agcaacttcc acggcacggc gacttcggtc acgtcgaagt cgtgatcggg gagcggcacg
    11281 aggatcttcg ccatgcgctc cgattgtgca cgaagctgtc agcgacggcg atggcgttct
    11341 gggaagcgtg agtgcccatg cgaacggtgc cgctcgcgcg tgcgctcctc ggatccgaac
    11401 gtgcggttgc caccgaacag acggccgggc tcttcgtctt cgaacattcg ggtgcgctcg
    11461 tcggtgaaac gttcgcggtc ggccgcgggc aacgcgccga cgaacgtacg aattcgctcc
    11521 tcgatgtgga cgaagcggtt gcgctcttcg cggtcgtcat tgtcatcgcg gacggtacgc
    11581 cggcgttcat cctcgtatcc gctcgtcacg aatcgatcgc gctcgtcgcg atcatcgtcg
    11641 tcgggcatct tgcggctgcg cgcgtccacg ttgcgctcct tgcgatgtgc catctctacc
    11701 tccccgtcgt gagcccgtgc tctcgaaacc ctctcgcctg cgcgtaacgc gagtcctgcg
    11761 atgtacgtgc cacggagacc tacgtcacgt cttcgaggtg cacaggcgtt gcaacgccac
    11821 ggcgacatgc cccgtaaaca gcgtttcaaa ccgagccgca agccgaagac ggaccaagcc
    11881 accgtttcga acccgcaggt ggatgaccgc aaggaaattc acccaaccac gcacgacatc
    11941 gagcgcgaag tgcccgtcga ccaagggcct cgcgacatcg aagccgagca gggttgagcg
    12001 agcgcatagg cgacgccgag cgctcccgcc cgagcgacgt aggtgcgtct cacctcacca
    12061 catcgggccg ccgccgaggt aatcggcgtg catgggtagg tcgaccgtag cgagcgactc
    12121 gcgattgctg gccgagtgct cggcgcagaa ccacacttct ccgtcggtat cggcacgcac
    12181 tgctgcgcac ttcggacact gcctcggcgc ctcgacgccg cgatcgatca tgtggacggc
    12241 gaacaccggg cagttcgcgt tttcgagaat gcggtgcgcg acgctaccga gcttgcgcca
    12301 cgagtgatgg gcgccaaacc gtccgcacac gatgagctgc gcctcgatgt cctcggccag
    12361 cccgatcact tcgtcggatg gcttgccggc gcgaatgtgc agccgcacgc gccactcggg
    12421 cccgcgatcg aatgactgga gcgtgtccat cacgagcgcg ccgagccggc gcttcgcgtc
    12481 ctcgatgcga gcttcgttgt cgcgagcgac gacgtgaatg acgtggatgt cgggggcgtg
    12541 gtggcgcgcg gcctgatcaa gcgcatgctc gaggacgatc tcggcgtgct cggacaggtc
    12601 gagcgcgacg acgatgcgga agtggggatc gcgctgcatg tcgaggatcg ctgcatccga
    12661 tgtgccgcaa tcgaccgcgc aagttcgcgc agcgcgtgcg tcagtggttt gcctgcgagg
    12721 ctttcggtgc aacaggtgcg ccggtcagga acgcgcggag cgctggttcc aatgctttgc
    12781 gctcggtgac gcaccactcg aagttgcgga tcgagaactg atcccacttc gggtcgatct
    12841 ggcggccgag cttctcgaca tcggcgcgct tggtgccatc gcagacctgg ttgaggaggc
    12901 ggactttctc gacatcttgc agcaacttga tctcggcgac atgacgttga agcagaccga
    12961 gcaagtcggg tgtgcgctcc agcgcgaatg cgatatccgt catgcgtgtg cgattcacct
    13021 tcggtagttg actaacaagc tcgtccgcaa acgctggctt gtcgaggatg gcagcgcgca
    13081 gcacgagcac gcgcgacgct gcgtcgaggt catcgagctt cggtagcagc ttcaatgcgt
    13141 cggcgcgcag cgtggggtcg ccagcgtcga gtacgagcgc gagcagctcg tacgcgtcac
    13201 ggcgcttctc gatcgataca ccggtaggcg cgatgccgat ggctcgtgcg cgcgacgcaa
    13261 agtgtttcgt gatccacgcg tcgtactgcg gacgcagcga ggcaggaatc tgcgggccga
    13321 gtccgtacaa ataggtggtg atccacttcg tcagatttgg atcatcgagc tcggccgctt
    13381 tttcgatgaa cgagagccgt aacgcgcctc gcgttccgtt ggccagtgcg acgctgatga
    13441 cggtcatgcg ctcgttcttc gtgagcatcg gccacgcccg gtcgcgcagc gcttcgagct
    13501 cctgcgctgt ccacgtcagg tggtgcaacg tcttcggcag cacccaccgc gggcaccgct
    13561 tcgttccgag ctcgagcgtg gcggaacccc atgcgagctt cgtacagcga tcggcgcgcg
    13621 ctccgtcatg atcgtaggcg acgcagagca gcccgaccgt atcgaaccct ttcacctcga
    13681 tgcgcttcga tttgccctcg caattcagct cgacgtcgag ctccgccgtc ggcgaggaca
    13741 ggtagcgttc gaatatgccc gcgaagtcgg tgccgagtga tggtttgagt gcatcgcgca
    13801 ggtcgtcgtt gcgaacggtg ccgtgggcgt gtgcggtgag atagcctcgc agcgcgcgct
    13861 tcagcggttc tgtaccgacg gtcgcatcga gcagcgcgag cagctgggcg cctttgaaga
    13921 cgctcgcgta cggcccaccg tgatgcgctc tcggatcggt gaccgcactg atgatcggca
    13981 cctcgccggg gtcgccggtc cagtaatagc tgtcgctgag ctcgccgccc agccagtacg
    14041 cgaagctctc gttgagccag aagtcgtccc accaggctgg tgtgacgaag tcgccgaacc
    14101 agtgatggac gatctcgtgg agaacgactt cctcccatcg gtctcgcagc gcattcgtgt
    14161 tgaacgtgat gagcccggcg ttctccatcg ccaaccacca gttctcggtg cttggcactg
    14221 cgacgagatc gagctttgga tagggaaacg ttagatcgag ccaggcttcg accttatcga
    14281 gcagagcagg tgtcgctgtc gcagccgcgc tgacgttctt gccctcacca cgcagcgcga
    14341 ggatgcggat cggtgtgccg tgcttggacg tgcccgcgtc gacgatatcg aatggaccga
    14401 cggcgaacgc gatcagatag ctcggcaacg gcagcgtgcg cgcgaaccgc actgtcttcg
    14461 tcgagtacgg accgccgctg cgcgtctcgc tctcgatcgc gctgttgctc gccgcgacca
    14521 ggccgtcggg gatgtcgagc gtgagctgcc acggcacctt gcgatcgggt tcgtcgaagc
    14581 aaggaaacac gccgcgcgcg tagtctggct cgaattgtgt gaatacgtac gcgtcctgct
    14641 tcggattggt ctcgttcgat gcgaggcgca gtccctgtgc ggtttcgcga aagccaccaa
    14701 cagcgtgacg ggtgttccac gtgccggtgt acgtgagcgt caatgtccag gtgcccgccg
    14761 cgagcggttc ttctgaccgc aaggcgagct cgggcgccga cgcctcgtta cggcgaccat
    14821 aggagagtgc gtacctcacc gtcagcggaa cctgcttgcc gtcgcgcgac gcggtcacgc
    14881 tcgtgattcg caggtcctcg gcgttgagcc agatcagctg cgatggttcc gagagcgctc
    14941 ccgtgatctc gatcgtgccc gagaactccg gctgcgccgg atcgacatgc agccgcgccg
    15001 cgtagctcgt cggctcgaac gtgtgcggca gtcgcttcgg gcgatctgcg ggcggtgccg
    15061 gctcggcagg tgccttggcc ggtgcgtcga ccttcggcgc gaccacgatc ggcggcgccg
    15121 gcggcggcat gctccgcgac gagcacgcgg tcaccgcgag cgcgacgagc gtcagctcac
    15181 caagcgtact gcttgaagaa cgcccactcg agctgcgtcg ccgactcgaa cgtcggacat
    15241 gcgaaggggc tcccgctctt gccacctgcc caacaatgac ccatcgacgt aaacgtgcaa
    15301 agggcaacct gcccgtcagc ggggcagccg tcgtaataga tgcacgagcc gccgttcaca
    15361 tccttgcgcg tgaccgtacc ggcgcagccg ttcttcctcg cccactcggt agccgacggc
    15421 gtgaggcctt gcggctgggg aacgctcgga tcgtcgcagc tgttcggcac gatggtgtct
    15481 gaggtgccat gaaagatgat gaccggcttg tgctgcgtcg tgcagctgtc gaacggatgt
    15541 gtgccgcccg agtgcggcgc gaccgcgcgg atgtcgtcgc gcatgcagcc ggtgtgatgc
    15601 gagaagtagc cgcccatcga gaaggcggtc acgtagatgt gatcgagatc gagacattga
    15661 tcggccgaga tgtcggcttt gatcgcgtcg agtagcgtga agtcgtcacc gggcgcgttc
    15721 ggcggcacgc caccgccctg cgcggggcac acgtcgatgc cgacgttcca tggggcgttg
    15781 agcgtgtcgg ggccgccttg accgtcggga tacgcgaccg cgatgtgctc ggcgtcggcg
    15841 agctcgttgt agccggtgat gtcgaacatc gcctggcccg acatcgtgaa gccgtggtgc
    15901 acgaacacga gcgggatgcg ttcctttgga tcgacgttcg gcggcagata cacgatgtac
    15961 gtgcgatcga gtgcggccgc gcggatcgtg cgatcggtct tgccgcgcat gccggtgcga
    16021 atgccgcaaa cgccgagcgc gtcgggatgc gtgtcgtcgg gccgtgcatc gggctttgtt
    16081 gccagcgtgt cttggccaca cgcggccaaa accaggcaca tgaaggcgcg tcgacacata
    16141 tttgccggtg aactctcgac gattttcgcc acccgatcca ccgagattcg cagacatgca
    16201 tgcggcacgg cggttgcgat aggtgcggac aaatgtcgtc acctcgcctc cgtcgtacgt
    16261 ctcgcctcgc cgacgccaag gtcgctttgt cgttggcgcg tgcggccttc gacaaggacc
    16321 gcccgctgat cgcgaacctc atcatcacgc gtcgttgcaa tctgagctgc ggctattgtt
    16381 acgaatatga caaggtctcg ccgccggttc cgctcgagac attgaaggag cgcatcgatc
    16441 acatcgcgcg gttgcgcgcg gtgttcgtga cgatgacagg cggcgaatcg ctgctcaatc
    16501 ccgacgccga caaggcagtg gcgtacatcc gcgagaaggg catggtgccg ttcctcaaca
    16561 cgaacgcgta tctgctcacg aagcagtgga tcgatcggct caatgcggcc ggcctatacg
    16621 gcatgcagat cagcatcgac aacgtgaacc cgaacgacgt gtcgaagaag tcgctcaaga
    16681 cgatcttgcc gaagctgaag ctgctcgcga agtacgcgcg gtttcgcgtc cgcatcaact
    16741 gcgtgctcgg ctcgagtccg cccgcggaag cgatcgaagt cgcgcgcacg gtgatcgggc
    16801 ttggctttga catgaacacg tcactcgtgc gcgccgagga cggcacgctg ctgccggtgt
    16861 cggaggaaac gcgcgcggcc tacgagacga tccgcggtct gggtaagcgc gcgccgttct
    16921 tcctcgatga cgactatacg aagccgctga tggacaacgg cgagagcgag tggaagtgcc
    16981 gagccggtgc gcgcacgttc cacgtcgatg aattcggcat ggtgcatctg tgcgcgccgc
    17041 gcgtcggatc gcccggtaag tcgatcgcgg agtacaccgc cctagacatt cgctactggt
    17101 tcaacgcgcc gaagtcgtgc gcgtcgaagt gcccggtcgc gtacgcgcgc catgcaagcc
    17161 gcctcgacgg ctggcgctcg cagaacggtg acctcatgca gctgcctgcg ccgcagccgc
    17221 agctcgtgca gttgcgcttg agccgcgcgg cctgatcacc gactcgtgac gtcggtgtcc
    17281 tcgccgaact cgaggccgag cgtgtgcatg tcgcggcgat ccatccgagc tgcacaggta
    17341 cgcggacagc cgacggcgca cgccggccgg aatcgcgagc acgccgcgat caccttgccg
    17401 acgggtgtct cctcgtactc gatcagctcc agatgggcgg tctcggggca ggtgacgagc
    17461 cggtacatgg aggtgtcacc agtgcagcgc gtgggccaga tcgaggcgct gcaagggttg
    17521 cagtgccccg accattcgcg cagcgggtgc gcgaatgcgg tcgcgctccg gcagtggatg
    17581 cgcaattagt ccccaatgct tggggcggcg ctatggagcg cgcggtcgcc agcggtcggc
    17641 ctcggtgtcc gtcggccact cgagctgacg caggtagtcg cgaaagtcgg tgcggtccca
    17701 ggcctcgagc gcgacgagca ccatccgcgg caggacgccg atgtagcggc ggccgacctt
    17761 cttgcgcagg gacagcttgt cgcggaattc gtcctcgatc tgctgggcga ccgcgtgctc
    17821 ggcatcgggg atgtacgcgt gcaccggctt gctcggatcg ttgcagtgcg ccggtggcgt
    17881 gtagacgtag agcgtgccgc atggcgtcgg ccgcgccgcg tagaccgtgc agcgctcgtt
    17941 gtcgtcgagg aacaggcacg gcacgcgcca ctcgtacggg ctcgccgcct ccatcgcctc
    18001 ggcgcgtgtg cgcaactcgt cgcgcagtgc gggcgtgtcg cgactcgctt gcttgaggtg
    18061 cgcggcgacg acgatgcctt cgtacaggcg cgcgacgaca accgagtgac agcacgcttt
    18121 cttcgcatcg cacttgaagc actgcacgac cggcagcttc tcctgcttgc cgaccttctt
    18181 cgcctcgctc gtcgcgcggg cgttgatgtc ggcgatcatc gtgcgcagcg attcgaggat
    18241 tccttcgtcg gcgacgaact gacctaacgt gcgggcctcc acgccgccat gctaaacgta
    18301 gttgtggccg gcgtgatcaa gaagaccgcg acgatcgatg ccggcgacgc gcagagcggc
    18361 ggttcggtgc gtctcgcagt tgtcgcagac acgcacagcg cgccgcatcc gaagactgcc
    18421 gagcggctcg ccgagctgaa gcccgacgcg atcttgcacg gcggcgacat cggcgatctg
    18481 caggtgctcg acgagctcgc gaaggtcgcg ccggtctacg cggtgcgcgg caacattgac
    18541 acgcacgcgc gcgacttgcc ggacgtgctc acgctcgagg tcggcaatgt gcggatcttg
    18601 ctcacgcaca tcgccgtcta tggaccgagg ctacgcggcg agatcgcgaa gctcgcacgc
    18661 gaggagcacg cgaagctcgt cgtgtgcggt cactcgcacg tgccgttcat cggtctcgac
    18721 aagggcatcg aggtgttcaa cccgggctcg atcggcccgc gtcgtttcaa tctgccgatc
    18781 ttgctcggcg cgatcgacat cacgccgacc agtctcaaga tgcgtcacat cgaagccgag
    18841 accggcaagc tctggctgcc gccgcggtag tccgcgccgc tagtcagtgc agcggaaccg
    18901 gtgccttcgt gaagaacttg ttcgccgtgc gggtcttgcc gccgagcgag tcgtagtcgc
    18961 ggtagccgac gacgatgagg ccgtcgccgt agagcgcgtc gcgcgcgcgg tcgatcgcct
    19021 tctggtcggc gcccgacgcg tcgaagtcaa tgccggtgat ctgcgccgac aggctcgagt
    19081 tgagcttctt ctcggtcagg ctcgggcacg gagcacgaac gcagcggatg ccattgtcgt
    19141 gaacgagcgc gaacacgccg tcgacccatt cgggcgcgct ggcgacccag atctcggtga
    19201 cgttgacgac gaggccgcga tcggccgggt ctggctcgac ggtgcccttg acgagcagcg
    19261 gcttgccggc gcggagatca tcctcgtagc cctgcgcgac cgacgccggc atcgcggtgc
    19321 cactccagtc gatcgtctgt accttgcact gcgactgcgc ggggccgcgg ccacactgga
    19381 tcgtcgagcg attcgcacgc gagacgaaga agccgttgcc gcaatcgccg gggctgtcga
    19441 ggctgcacgc gcgggtgtcg gggatcaggt tgaggaacgt gaaggtcgcg gcgctgtcgg
    19501 ccttgccgcc ctcgccatcg gcgctctcgt cggagagctc gtcggcgggc tgcgaggtgc
    19561 aagcggggac cagaatcgaa gtcatcaagc caagaaatag agcggaacgc atgatgcgcg
    19621 cgccatgtgc atcgcccagg ccacgtagcg cacgtggaat ctcgatgggg tagagggttc
    19681 agatcgtggc cagtccgctg gccggcggct agtcccgcct tgatgcgctt gcgttacacg
    19741 gcccatcaac gcgatagcgc tcgggatctt tgaagcgcga aaccggcgct cgcttatgtg
    19801 cgcgtgcttc ggcgagcgcg ttcatttcgt cgagcggcca atgctcgtga gcgcgtattg
    19861 catcgagcag cgcgacgaac gattcgacgc tgccggtgac gctgcgatca ctgttcgtga
    19921 gcacgtcacg ttccggtccg accagacgct gcgcgtactc ctgcgtcgac cagcacgagt
    19981 cgagcacgat caatcggtag tcgtgtggtg gcgagatatc gatcgcgccg tcgtgactgt
    20041 ggccgttgta gtagacgacg tcgtggcgcg cgagtgcacc ggcgagcgct gcgctcgttt
    20101 gtgcggggtc gtcgggcacg agtgcgagat cgatcgtcac gttgccgagc gtgaaccggt
    20161 cgccgtcttc ggcaaagcca cgatcgcgga gcgcgcttgc gaacgcgcga tagctccaca
    20221 gattgtggtc ggtgggtccg ccctgcagct cgccgaacac cgctgcgacg gtgatgcgac
    20281 catccgcgag catcgcgtca tagcgcgggc ctctactgtc cgcgcggcac acgccgtcgg
    20341 gaatgtcagg cgcgcgcgtc tgcgcgtaga cagtgatcgc gcacgcgatc aaaagcccga
    20401 caaacccgag atcgatcgct agacgagtcg ccgcacgatc agccatagcg ctccgatgcc
    20461 gaagaagacg agaatccacc aatcgccgag cgaccggccg ccaccgcccc agccaccgaa
    20521 gccaccacca tcgtcgtacg tcgagcgacg cgacgacgac ggagccggat cgggcgcttc
    20581 gttgacggtc gttgccgggt tcacgccctc gggcacgacc gacggctgct cgacgacccg
    20641 cacctggccg ttcgacacga tgcgcgtgcg atcgaccgcg acgaacgacg tgaactcggt
    20701 gacgaggtga aagcggaggc ccagctcggt gaccggcttg cgcacgtctt cctcgtcggc
    20761 tttgtccatc agcacgtcga tcttgcggcg cgcccacagc gacgcgaccg gctcgaagtt
    20821 gttgaccggc ggcagcgtga ccgcgatcgg aatcgtcacg cgcttgcccg cggtcgtcgc
    20881 gctcaccttg atcacggcct ggccgggacg gcgatagcgg ccggtgacga tgagcggctg
    20941 gccggcatag acgtcgggca ccatcgcggg cgtcaattcc tcgacggcga gcccacccca
    21001 atcgacctgc acgtcggtca tgtacgggta cgcggacttg aacacgaggt tcttcgcgag
    21061 cttctcggcg gactcggtga gcgtcaggta gctcgcgaag ccacggcccg cgccggcgag
    21121 cttgtcgagc aagctccggt tcggcgccga gccaatgccg acggtgaaga tgcggttcgt
    21181 gccgaggaag cgcttcgctg caccgacgac gacatcgtcg ttgccgacga acccgtcggt
    21241 gaggaagtag acgacgcgaa tgcgatcgct gcccggcgtc gcggtgagca tgcgcacgac
    21301 gccttgctcc atctcggtgc cgccgccgct ctgcagcact ttcaagtact cgaggccgcg
    21361 ctgcttgccc gcgccatcgc cgcgaatcgg attatcggcc atcgcctcga ccccgctcgc
    21421 aaacgcgacg acgttgaacg agtcgcgctc ggtcagcgta tcgatgatcg cgctcgccac
    21481 cgccttcgcc tgcgcgagcg gttggccgtc catcgagccc gaccgatcga tcacgatcat
    21541 cacttcgcgc ggggtgatgt cgccggtgcg gtacgtcgcc ttcggctgca ccgcgagcat
    21601 gaagtagcca tcctcgtcga ccttgtgcgt gagcaggccg accgtcgtct gtggaccggc
    21661 ggtcttgtag cgaatcacga ggtcgcggtt cggaatctcg tcgctgcgac cgagcgtgat
    21721 gtgactgcgc gtgacggcgg gctgcgtcgc gagaatgtcg tgcgacggac tttcgacacc
    21781 gacgatcgga acacctgcat cgatgtcggc ggtgaagctc accgtgctgc tcgcgcgctc
    21841 cgagctcacg tacggcatcg acgccgcgct caccggattg cgcccttgcc gatcggcggg
    21901 caggtagcgc gggccgaccg tgagcggtac cgccatcgtg tacgcgccgt tgtcgtaatc
    21961 gagtagctcg acgtagcgga tccggatctt gatcgtctcg tgtggcgcga tgttcgcgat
    22021 gtgctgcgtg aagatgttcg gcttgttctg ctcgacgagg cctgcggtgt gcccgctgct
    22081 cttcgcttcg tcgtagatgc ggcgcgcgtc ttccttcgtc ttgatctcgc cggtgatcgt
    22141 gcgctcgccg atgccgatct cgtagccgga gaccgcgccg tcgtcgccga gcgggaacac
    22201 gtagacggcc tcgatcggct cgtcgaacgg gttctcgaag atctgctcga cgttgtagac
    22261 cgccatcatg ccgccgacgt tcgcggtgat cgcggtgtgc ttgagcggga agccgatcac
    22321 ttcctgcttc acgccttcga acaccgcgac ttgcggcgcg acgaggcgta gctcgccacc
    22381 ggcgacggga atctcgtcca ttgccgcgta gccgacgctc ccgctgatcg tcacgtcggc
    22441 agacatcgtc attcccgcgt gacacgacgc gagcaacgcc agaacgagca agctgcgcat
    22501 gggatcctcc gggcgtgcgt gtgcagcaac tcacgcgccg atcacgcttt gacgagattc
    22561 accacggcct gcccgagacg tgcgcgcgct gccgcgattt ccgtgacagc gatcgaacgt
    22621 cgcgtcgaac gtctaccgct tagcttgccg gcgagcgcgc gctgcggcgt agacctgctt
    22681 cgcgcgcgtc gcgtcggcgt ggttcggcgt gttgtggagg atcttgtcca tttcgagctc
    22741 ggcctcgtcg agccgaccga gcatgccgag tgcgcggcca cggacgaagt gcgcgtagtc
    22801 gctgccgggc gcgagctgca gcgcggcgtc ggcggcttcc agtgcatcgg ccggccgctt
    22861 gcgcgcgcac agcacgttca ctttctcggt gagcaagccg ccgtgccagc gcgcatcgag
    22921 tggcagcgcc aacgcgcgcg acagctcggc ctcggcttcg tcgatccgat cgaggcgcac
    22981 gagcaagcgc acccagctgt gccaccacat gccgccgtcg agctcgcctt gcacggcgcg
    23041 ctggagcagc ggcaccgcgt cggcctgctc cggcgtatcg gcgagcacgg cgccgagcca
    23101 gccgtgtgtc gatggcgttt gatcgccgag cgcgatcgcg cgtttgagga gcgtcgccgc
    23161 gtgctcgggg aacgacgcga agaacagctg gcggcctttc tccgcgtacg cggcggggtc
    23221 gtcgggcttc gcttcgatcg cgagatcgag cagtgcaagc gcgcgccgca ccagaccgcg
    23281 tgtgtgtgcg tcgcgagctg cggcgagcgg atcggccggc agtgcaccaa ccgccgcgcg
    23341 cgcgttcgt
//
LOCUS       DASUFX010000109         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_17955,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000109 DASUFX010000000
VERSION     DASUFX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17955"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFV99_04200"
     CDS             complement(<1..309)
                     /locus_tag="VFV99_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058535.1"
                     /translation="MQRSLVALVLAAIVLVVYAPIVLGGRTWDDARYHTEVAPPRIAA
                     ADAVLHGELPTWWEGTALGVPLLAEPSHGAAYPLTWIAATPLTFDLAVIAHILWCALG
                     V"
     gene            380..712
                     /locus_tag="VFV99_04205"
     CDS             380..712
                     /locus_tag="VFV99_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058536.1"
                     /translation="MLVAVLAAGCGHKKDEASCGAVATRLFTLAHDDLGSATVDPAIR
                     RAVADQLPAMRDSLTQICTDGKWSTQVRNCMVSAGDHLALQACQQHLTDEQRRALDRA
                     TVGETTSH"
     gene            740..1048
                     /locus_tag="VFV99_04210"
     CDS             740..1048
                     /locus_tag="VFV99_04210"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX5058537.1"
                     /translation="MEIDFQYSLPDPDAKSRLEILGQYLANKHGIMVTWLDGKRAKFS
                     GKYLVVKIDGELSIGNGRAQFKGEDPGFLWRNRAKDYIQSKLAKYLDPSLQPAQLPTS
                     "
     gene            complement(1131..1271)
                     /locus_tag="VFV99_04215"
     CDS             complement(1131..1271)
                     /locus_tag="VFV99_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058538.1"
                     /translation="MKAQNEMSVWSIIERLQRRLERQGMSAAEADRAVAAALKSLALA
                     RA"
     gene            1395..2162
                     /gene="xth"
                     /locus_tag="VFV99_04220"
     CDS             1395..2162
                     /gene="xth"
                     /locus_tag="VFV99_04220"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5058539.1"
                     /translation="MKLVTWNINSIRARTEKLLTWIGNERPDILCLQETKVEDSGFPL
                     EALRARGYEVVTYGQRSYNGVAIAARSPLTDVVRGFGDGQPDEEARLIAATVDGIRVV
                     CVYVPNGQDLTSDKFPYKLAWYRRLRAFLDRTVKTTDNVVVCGDMNVTADDRDVCDPV
                     KWAGHIHCSADERAALGELVGFPLVDLFRKHHPNGGVYSWWDYRGVAFFKDQGLRIDY
                     IFATQPVADRCTACTIDRNARKGQDASDHAPVIATIE"
     gene            complement(2201..>2912)
                     /locus_tag="VFV99_04225"
     CDS             complement(2201..>2912)
                     /locus_tag="VFV99_04225"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5058540.1"
                     /translation="IAGFMVWLMTPVERMLLGRVSPNAVTAVSLAMCIITGVAAGMGA
                     LAAAMWLYTFGGVLDILDGRLARLSGKQTAAGALFDSVSDRWGELFVFGGYAWYLRDT
                     PWLLAAFGAFGASMMVSYTRARAESLGIELSAGMMQRAERIVVVVFGTLGALLYGDVE
                     AAVPIIGITMLICAATSAATAINRWIIAFRELSKRTAPVVVEEAPAMSLPVVASIAPA
                     ELRKSAPELRKVRPLEQH"
BASE COUNT          559 a          952 c          920 g          481 t
ORIGIN      
        1 cacgccgagc gcgcaccaca ggatgtgcgc gatcacggcg agatcgaacg tcagcggcgt
       61 tgctgcgatc catgtcagcg gatacgccgc gccgtgactc ggctccgcga gcagcggcac
      121 gccgagcgcg gttccttccc accacgtcgg cagctcgccg tgcagcaccg catcggcggc
      181 cgcgatgcgc ggcggcgcga cctcggtgtg gtagcgcgcg tcgtcccacg tccgaccgcc
      241 gagcacgatc ggcgcgtaga cgacgagtac gatcgcggcc agcacgagcg cgactagcga
      301 tcgctgcacg cggcgactat agggccgccg acttgacagc tggagcgcgc gagacgaaaa
      361 cctcgccatg cgctgtctcg tgctcgtcgc ggttcttgcc gctggctgcg gtcacaagaa
      421 agacgaggcc agctgtggcg ccgtcgcaac gcggctgttc acgctcgcgc acgacgatct
      481 cggcagtgcg acggtcgatc ccgccatccg tcgggccgtc gctgatcagc tgccggcgat
      541 gcgagactca ttgacccaga tttgtacaga tgggaaatgg tctacgcagg tgcgtaactg
      601 catggtcagt gctggcgacc atcttgcgct ccaggcctgc cagcagcatt tgaccgacga
      661 gcaacgtcgc gcacttgacc gagccactgt cggcgagacg acctcgcatt agacgtgcta
      721 agaacgttga accaacgcga tggagatcga ctttcagtac tccctcccgg accccgacgc
      781 gaaatcgcgc ctcgagatcc tcggtcaata cctcgcgaac aagcacggca tcatggtgac
      841 gtggctcgac ggaaaacggg ccaagttctc cggcaagtac ctcgtcgtga agatcgacgg
      901 tgagctgagc atcggcaatg gccgcgcgca gttcaaaggc gaagaccctg ggttcctctg
      961 gcgcaatcgc gcaaaggact acatccagag caagctcgcc aagtacctcg atccgagctt
     1021 acagcctgcg caattaccga cgagctgacg gtcgatcgtc gctcgggagc cggattgaac
     1081 tggccgttct agtggactga cggttctact gagacggacc ggtgccagtc ttacgcgcgt
     1141 gcgagcgcga gtgacttgag cgcagcggcg acggcgcgat cggcctcggc ggccgacatg
     1201 ccttgccgct cgaggcggcg ttgcagacgc tcgatgatgc tccacacgga catttcgttc
     1261 tgagccttca tgttgatcct cacgtatgag ggctgatgtc ggtctaacag cagggggact
     1321 gccctcattc ccgaagacag atcacgagtt ttctggttgc agcactcgct ggtagtccga
     1381 tgtaggttcg cgttgtgaag ctcgtgacct ggaacatcaa ttcgatccgc gcgcggaccg
     1441 agaaactgct gacgtggatc ggcaacgagc gtcccgacat cctgtgcctc caggaaacca
     1501 aggtcgagga ctccgggttt cccctcgaag ctttgcgtgc gcgcggctac gaagtcgtca
     1561 cctacggcca gcgcagctac aacggcgtcg cgatcgcagc gcggtcacca ctcacggacg
     1621 tggttcgcgg cttcggtgat ggccagcccg atgaagaagc gcggttgatc gcggcgacgg
     1681 tcgacggcat tcgtgtcgtc tgcgtctacg tgccgaacgg tcaggatctg acgagcgaca
     1741 agtttccgta caagctcgcc tggtatcgcc ggctgcgcgc gtttctcgat cgcacggtga
     1801 agacgacgga caacgtcgtc gtgtgcggcg acatgaacgt caccgccgac gatcgtgacg
     1861 tctgcgatcc ggtgaagtgg gccggccaca ttcactgcag cgccgacgaa cgcgcagcac
     1921 tcggcgagct cgtcgggttc ccgctcgtcg atctatttcg caagcaccac ccgaatggtg
     1981 gcgtctattc gtggtgggac tatcgcggcg tcgcgttctt caaggatcag ggcctgcgca
     2041 tcgactacat cttcgcgacg cagccggtcg ccgaccgctg caccgcgtgc acgatcgatc
     2101 gcaacgcgcg caaaggtcag gacgcgagcg atcacgcacc ggtcatcgcg acgatcgagt
     2161 agagatccga ttctggccga agtccgaagc ccgaagcctg tcaatgctgc tcgagcggac
     2221 gaaccttgcg cagctcgggc gctgacttcc gcagctccgc cggcgcgatc gaagcaacaa
     2281 ccggcagcga catcgccggc gcttcttcga cgaccaccgg cgcggtgcgc ttgctgagct
     2341 cgcggaacgc gatgatccag cgattgatcg cggtcgccgc cgacgtcgcc gcgcagatca
     2401 gcatcgtgat gccgatgatc ggcactgccg cctcgacatc gccgtagagc agcgcgccga
     2461 gtgtgccgaa cacgacgacg acgatgcgct ctgcacgctg catcatgccg gccgacagct
     2521 cgatgccgag actctcggca cgcgcgcgcg tgtagctcac catcatcgac gcgccgaacg
     2581 cgccaaacgc agcgagcagc cacggcgtgt cgcgcaggta ccaggcgtag ccgccgaaca
     2641 cgaagagctc gccccagcga tcgctcaccg aatcgaacaa cgcacctgca gcggtctgct
     2701 tgccactgag gcgcgcgagc cgaccgtcga gaatgtcgag cacgccaccg aacgtgtaga
     2761 gccacatcgc cgcggcaagc gcgcccatcc cggcggcgac gccggtgatg atgcacatcg
     2821 cgagcgacac tgcggtcact gcgttgggag acacacgacc gagcagcatt cgctcgacgg
     2881 gggtcattag ccagaccatg aacccggcga tg
//
LOCUS       DASUFX010000110         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1824795, whole genome shotgun sequence.
ACCESSION   DASUFX010000110 DASUFX010000000
VERSION     DASUFX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824795"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /gene="hscA"
                     /locus_tag="VFV99_04230"
     CDS             complement(<1..1746)
                     /gene="hscA"
                     /locus_tag="VFV99_04230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX5058541.1"
                     /translation="MLMQISEPGTSQVKEACKPRVVGIDLGTTNSLVAHVEDGGPKVL
                     GDDPIVPSVVWYAPSGGTLVGRRAQAMAVEEPGRTLASVKRLIGRGMKDVESVRGMLP
                     YELVGDDRAVRMRVGGRELSPVEVSAEILKELKSRAEEQLGGTIEGVVITVPAYFDDA
                     QRQATRDAGRLAGLEVMRLMAEPTAAAVAYGLDRGSKGVYAVFDLGGGTFDISILKLV
                     EGVFEVKATGGDSALGGDDLDRAIAQDRFAADIASGDRHRIATAVARARSLKERLTDA
                     PEVDGFTRDDLRRIAQPLLDKCGKACRRVLKDAELGKDDLDGVILVGGSTRSPVVRAF
                     VRELFGKEPLHDLDPDQVVALGAAVQADALAGGEQQVVLLDVVPLSLGVEMMGGVVEK
                     LVYRNTTIPCGATQTFTTYADNQTGFDIHIVQGERETVDACRSLGRFTLRGIPPMIAG
                     LARLEVTFLIDADGLLKVMAKELTTGKEASIEVKPSYGLSDEEVERMLLESFEHAEED
                     LEKRNLAVERVEAERILAATKQAFETDAALLTDDVRAAGEQAMTALDAAMKSPDYLAI
                     RHAVEALDIATKPFAQ"
     gene            complement(1748..2269)
                     /gene="hscB"
                     /locus_tag="VFV99_04235"
     CDS             complement(1748..2269)
                     /gene="hscB"
                     /locus_tag="VFV99_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEX5058542.1"
                     /translation="MERASSSNDAFGLLGLPAQFDLDPGVIERAFFDKSKELHPDRFA
                     NSPAAERVAALSKSRALNDAYQTLKKPVQRAEYLLERAGVSIGDNERLDPTFLMEILE
                     LREELAEARVAKRTSEIEKLCGVMKARRKATVDSLTALFAKNDLPAIKEQLIVLRYLD
                     RYLEECDNALDED"
     gene            complement(2247..2633)
                     /locus_tag="VFV99_04240"
     CDS             complement(2247..2633)
                     /locus_tag="VFV99_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5058543.1"
                     /translation="MAAAPQSDTSTSAGPPAVGKKEIAITEAAAQEIARQREKRGTPN
                     ARIRVGVRGGGCTGFTYVFEWADEVKPTDSEFADHGVAIVVDPKSLVYLGGMTLDFVR
                     GMMGHGFKFNNPNAKGSCGCGESVQF"
     gene            complement(2644..3036)
                     /gene="iscU"
                     /locus_tag="VFV99_04245"
     CDS             complement(2644..3036)
                     /gene="iscU"
                     /locus_tag="VFV99_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEX5058544.1"
                     /translation="MAYSDKVIDHYENPRNVGTFKNETDVSKDEVGTGLVGAPACGDV
                     MRLEIRVVDGLIADARFKTFGCGSAIASSSLATEMIKGMTLDQAAGIKNTQIVEELNL
                     PPVKIHCSVLAEDAIKAAIDDYKKKHSR"
BASE COUNT          489 a         1072 c          999 g          565 t
ORIGIN      
        1 ctgcgcgaac ggcttcgtcg cgatgtcgag cgcctcgacg gcgtgacgaa tggcgagata
       61 gtcggggctc ttcatcgcag catcgagcgc agtcatcgcc tgctcgcccg cggcgcggac
      121 atcgtccgtg agtagcgcgg catcggtctc gaacgcctgc ttcgtcgcag cgaggatgcg
      181 ctcggcttcg acacgctcga ccgcgaggtt gcgtttctcg agatcctctt ccgcgtgctc
      241 gaacgactcg agcaacatgc gctcgacttc ctcgtcggag aggccgtagc tcggcttgac
      301 ctcgatcgag gcctccttgc cggtggtcag ctccttcgcc atgaccttga gcaggccatc
      361 ggcgtcgatc agaaacgtga cctcgagccg cgccaggccc gcgatcatcg gcgggatgcc
      421 gcgcagcgtg aaccggccga gcgaacgaca cgcatcgacc gtctcgcgct caccttgcac
      481 gatgtggatg tcgaaaccgg tctggttgtc ggcgtacgtc gtgaacgtct gcgtcgcgcc
      541 gcacggaatc gtcgtgttgc ggtagacgag cttttcgacg acaccgccca tcatctcgac
      601 gccgagcgac agcggcacga cgtcgagcag cacgacctgt tgctcgccgc ccgcgagcgc
      661 gtcggcctga accgccgcgc cgagcgcgac gacttgatcg ggatcgagat cgtggagcgg
      721 ctccttgccg aacagctcgc gtacgaaggc gcgcacgacc ggcgagcgcg tcgagccacc
      781 gacgaggatc acgccgtcga gatcgtcctt gcccagctcg gcatccttga gcacgcggcg
      841 acaggccttg ccgcacttgt cgagcaacgg ctgcgcgatc cggcgcagat cgtcgcgcgt
      901 gaagccatcg acctcgggcg catcggtgag acgctccttg aggctgcgcg cacgcgcgac
      961 ggcagtggcg atgcgatgcc gatcgcccga cgcgatgtca gccgcgaagc ggtcctgtgc
     1021 gatcgcgcga tcgagatcgt cgccgccgag tgcggagtcg ccgccggtgg ccttcacttc
     1081 gaacacacct tcgacgagct tgagaatcga gatatcgaac gtaccgccgc cgagatcgaa
     1141 cacggcgtag acgcccttgc tgccgcgatc gagaccatag gcgacggccg cagcggtggg
     1201 ctcggccatc aggcgcatga cctcgagacc agcgagccgg cccgcatcgc gcgtggcctg
     1261 gcgctgcgca tcgtcgaagt acgcgggcac cgtgatgacc acgccttcga tcgtgccgcc
     1321 gagctgctcc tcggcgcgcg acttgagctc cttgaggatc tcggccgaga cctcgaccgg
     1381 cgatagctcg cggccgccga cgcgcatgcg caccgcgcgg tcgtcaccga ccagctcgta
     1441 cggcagcatg ccgcggacag actcgacgtc cttcatgccg cggccgatca gccgcttcac
     1501 cgaggcgagc gtgcgtcccg gctcctcgac cgccatggct tgcgcgcggc gaccgaccag
     1561 cgtgccgccg ctcggcgcgt accacacgac cgagggcacg atcggatcgt caccgagcac
     1621 cttcgggcca ccgtcctcga cgtgtgcgac gaggctgttc gtcgtaccga gatcgattcc
     1681 gacgacgcgc ggcttgcagg cctctttgac ctgcgacgta ccgggctcgg agatttgcat
     1741 caacatctta gtcctcgtcg agtgcgttgt cgcactcctc gagatagcgg tcgaggtagc
     1801 gcagcacgat cagctgctcc ttgatcgccg gcagatcgtt cttcgcgaac agcgcggtca
     1861 gcgaatcgac ggtcgccttg cggcgagcct tcatcacgcc gcagagtttt tcgatctcgc
     1921 tcgtgcgctt cgcgacgcgc gcctccgcga gctcttcgcg cagctcgagg atctccatca
     1981 ggaacgtcgg atcgaggcgc tcgttgtcgc cgatcgagac gccggcgcgc tcgagcagat
     2041 actccgcgcg ctgcaccggc ttcttcagcg tctggtacgc gtcgttgagc gcgcgcgact
     2101 tcgacagcgc agcgacccgc tccgccgccg gcgaattcgc gaaccggtcg gggtgcagct
     2161 ccttgctctt gtcgaagaac gcgcgttcga tcacgccggg atcgagatcg aactgcgctg
     2221 gcaaaccgag cagcccgaat gcgtcgttag aactggacgc tctctccaca gccgcacgag
     2281 cccttcgcgt tcgggttgtt gaacttgaac ccgtgaccca tcatgccgcg cacgaagtcg
     2341 agggtcatgc caccgagata cacgaggctc ttcggatcga cgacgatcgc aacgccgtgg
     2401 tcggcgaact cactgtcggt gggcttcact tcgtcggccc actcgaacac atacgtgaag
     2461 cccgtgcagc cgccgccgcg cacacccacg cgaatgcgcg cgttcggcgt gccgcgcttc
     2521 tcgcgctgac gcgcgatttc ctgcgctgcc gcctcggtga tcgcgatctc cttcttgccc
     2581 acggccgggg ggcccgcgga cgtcgaagtg tcgctctggg gtgctgctgc catcatcatg
     2641 gccttacctc gagtgcttct tcttgtagtc gtcgatcgcc gccttgatcg catcctcggc
     2701 gagcaccgag cagtggatct tcaccggcgg gaggttcagc tcctcgacga tctgcgtgtt
     2761 cttgatcccg gccgcctgat cgagggtcat gcccttgatc atctcggtcg cgagcgagct
     2821 cgacgcgatc gccgagccgc agccgaacgt cttgaagcgc gcgtcggcga tcaggccgtc
     2881 cacgacgcgg atctcgaggc gcatgacatc gccgcacgcg ggtgcgccga cgagcccggt
     2941 gccgacctcg tccttcgaga cgtccgtctc gttcttgaac gtgcccacgt tgcgggggtt
     3001 ttcgtagtgg tcgatgacct tgtcggagta cgccatggtg gtttctctct gcttgtgcag
     3061 ctcagtgcgg ggtccactgg atcgagttga ggtcgatgcc gtcctggtgc atctcccaga
     3121 gcggc
//
LOCUS       DASUFX010000111         7096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_852454,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000111 DASUFX010000000
VERSION     DASUFX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852454"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFV99_04250"
     CDS             <1..1191
                     /locus_tag="VFV99_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058545.1"
                     /translation="LWEDTGRWLGSSVGPNISDVTIEVEGYRNGKEHHTYLMPVMRHD
                     NFTDKTADIKIEKILVPVGNQTENGQLKLVTLKELLENPEQYMSTRDKGKIKNSTLLA
                     KRDTHVLVSAQHAFLPVPQEGQAKFWPVIFNYQSTKKNPAVLAILVTRQGTSMTIIDN
                     NRDTVTGGDSWGQRLYFNQSGQKAPLIAERLTDVKNKGTTANGEDAQSLGADANVLML
                     IQVPLKYKEPPRPKYGGAPMAMGLADGGGGGGGYAAPAKMAKEAQASRAGGDRERSDV
                     DTAVLGHGATEGPYTELDGLTIQRDSRFPVRVTLQFYQATSNGVISKTNVAAMAAQIK
                     KVYGKGDYVGSLVVPTELDRLRPTNWVGVTEAPLDVWNHLPGLLERIGQYGWRRPQMR
                     AGVM"
     gene            complement(1196..2272)
                     /locus_tag="VFV99_04255"
     CDS             complement(1196..2272)
                     /locus_tag="VFV99_04255"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5058546.1"
                     /translation="MRWLAAVACVVGCASSAENPKPVTKAELGEQLFNDPKLSEPAGQ
                     ACADCHDPKATFVDPEFERVSPGVLRERVGTRNSPTVLYSQFTPPLHKDASGKLLGGQ
                     FWDGRANTLEEQAAFPLVNPLEMNNPSKAAVVAKVKKAYGAQFRQLYGKDAFADTDKA
                     FALVGDAIAAFERTPQMAPFSSKYDKYLAGTAQLTDSEARGLAVFEDPAKGNCASCHP
                     NRPGADGSPPLFTTYTYENLGLPKFADSPFYGLAKDLNPDGEAFIDHGLMTTTNDPAH
                     DGMFRTPTLRNVGKTSPYGHNGYIHRLDEFVEFISGSCRRVGACKWPEPEVATNAQRV
                     RTGGKLTPQEINDLVAFLRTLDDD"
     gene            complement(2315..3406)
                     /gene="apbC"
                     /locus_tag="VFV99_04260"
     CDS             complement(2315..3406)
                     /gene="apbC"
                     /locus_tag="VFV99_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX5058547.1"
                     /translation="MAITEAQVREALAKVNDPAFDKDIVSYRILSKVEVSGDDVLVRL
                     DIPTAAYPLALRKELIARVDTAVKALGAKRTTIIPEVNTAFMPAPSDKAVLKGPKNVI
                     AVAAGKGGVGKSTVAVNLALALQRHGAKVGLLDADVFGPSIPTMLGSPEVPPQASKES
                     RIIPAIHHGVKVISVGFFVDKDEAVVWRGPMVHRLLQQFLGDVDWGELDYLVCDLPPG
                     TGDVQLSLSQLIPIAGAVMVTTPQEVSIVDVVKGIAMFEKVEIPILGIVENMSYYKCP
                     ACGHHDEIFSHGGGKRLAQEVGTEFFGEIPIDTRIRFGGDAGVPVIIASPDSENAKLF
                     MDLATKAALAVAKSVLSKPKRSPRLAVIK"
     gene            complement(3424..4035)
                     /gene="ribA"
                     /locus_tag="VFV99_04265"
     CDS             complement(3424..4035)
                     /gene="ribA"
                     /locus_tag="VFV99_04265"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5058548.1"
                     /translation="MLTRYSDARLPTPHGEFRVVVYRTGEPGGAGASAVGLENEEHVA
                     MVLGDVTGQAVLTRVHSSCFTGEVMGSLRCDCRAQLEGALARIAREGRGVLVYLVQEG
                     RGIGLGNKVRAYQLQDNGHDTVEANLALGFDADLRSYDLAAGILRDLGVTSVRLMTNN
                     PKKAAGLEHAGVIVDALEAHWVGESEHNADYLNAKKLKLGHLP"
     gene            4097..4639
                     /gene="def"
                     /locus_tag="VFV99_04270"
     CDS             4097..4639
                     /gene="def"
                     /locus_tag="VFV99_04270"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5058549.1"
                     /translation="MAVRPTVIWPDPRLREPTVEITTIDDAVRELYRDLCDSMYAENG
                     LGMAAPQLGEARKMFIVEPKLAGLAETDPPLCFMNPEVLETSEEMQDSEEGCLSFPDI
                     YIKVKRPMRCKVRALGIDGKQFTIEGEGLLARCLLHENDHLTGKLLVDFVGPLKRQMI
                     KKTLQRHKDAEAEELEEAEA"
     gene            complement(4636..5220)
                     /locus_tag="VFV99_04275"
     CDS             complement(4636..5220)
                     /locus_tag="VFV99_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058550.1"
                     /translation="MRLAWVVVLAGCYTKAPPATISNRAEPSKRGLLTITEDGFGPLT
                     AGSKATLTALRSAFAGYDVRAANDSGLSYSVYLGDEKLAWVIPNESDGSLFNVHATSP
                     RVETFGHDWRVGQSFQGAATLTDCECWGDNPTCYRRGGHVAVNFKRSCEGLVGSDARA
                     LRVLDGVEIQRVIWSPKPFAGSSSEEYAVPSQAP"
     gene            5261..5668
                     /gene="folB"
                     /locus_tag="VFV99_04280"
     CDS             5261..5668
                     /gene="folB"
                     /locus_tag="VFV99_04280"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5058551.1"
                     /translation="MTDSVFVRGLEFEGNHGYTAAERRGTRRFRVNLTLELSLAAAAV
                     SDKISDTIDYWKVSEAVVAIGTKSTFKLLEALAGAIGSRIQELYPRAAVEIELEKLAP
                     PCPGVPAACGVKLVFPARQSESGGLRLGPAKEA"
     gene            5689..6099
                     /locus_tag="VFV99_04285"
     CDS             5689..6099
                     /locus_tag="VFV99_04285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5058552.1"
                     /translation="MRFADVDHAGIVYYPRFFHYFHVAFEELWRARIGPQAYSDIIDR
                     DRIGFPAVRAECDFKAPLKFGDTAEIDVTIPRLGTKSITFRYRIYRGEAVPSGTRVLC
                     AEGQVVCAVVDLAKFAAVAIPEHVTNMLRDLVES"
     gene            6213..6944
                     /locus_tag="VFV99_04290"
     CDS             6213..6944
                     /locus_tag="VFV99_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX5058553.1"
                     /translation="MVTALIEHRVSTSLVDVERALAKWRSGQHSALAAHGAVLRHAAR
                     CERTVERVTAAAGDRPEGILRDAVDAGLMSSEEFVQLVGKQPNEVPSIDALRDEDDAS
                     APDKRRTLETLLARGPVLVHVDARRAEVSVPPRFRADPSLVLRFGYTLSPAIADLGVD
                     DNAISGTLTFGGQPFHCVLPWTAVYAAMVEGEQRGTVWPEDVPEDVLTGGGEAPAPLP
                     AAQPQQQAEPQPQRAKRTSHLKLVE"
BASE COUNT         1248 a         2379 c         2262 g         1207 t
ORIGIN      
        1 ctctgggagg acaccggtcg ctggctcggc agctcggtcg gtccgaacat cagcgacgtc
       61 acgatcgagg tcgagggcta tcgcaacggc aaggagcacc acacgtacct gatgccggtg
      121 atgcgccacg acaacttcac cgacaagacc gccgacatca agatcgagaa gatcctcgtg
      181 ccggtcggca accagaccga gaacggccag ctcaagctcg tcacgctcaa ggagctgctc
      241 gaaaaccccg agcagtacat gtcgacgcgc gacaagggca agatcaagaa ttcgacgctg
      301 ctcgcgaagc gcgacacgca cgtgctcgtc agcgcgcagc atgcgttcct gccggtgccg
      361 caagaaggtc aggcgaagtt ctggccggtc atcttcaact atcaatcgac gaagaagaac
      421 ccggccgtgc tcgcgatcct cgtcacgcgc cagggcacga gcatgacgat catcgacaac
      481 aaccgcgaca ccgttaccgg cggtgactcg tggggccagc gcctctactt caaccagtca
      541 ggacagaagg cgccgctcat cgccgagcgc ctcaccgacg tgaagaacaa gggcacgacc
      601 gcgaacggcg aggacgcgca gagcctcggc gctgacgcga acgtgctgat gctgatccag
      661 gtgccgctca agtacaaaga gccgccgcgc ccgaagtacg gaggcgcacc gatggcgatg
      721 ggcctcgccg acggcggcgg aggtggcggc ggctacgcgg caccagcgaa gatggcgaag
      781 gaagcgcagg cgtcgcgcgc gggtggcgat cgcgagcgct cggacgtcga caccgcggtg
      841 ctcggccacg gggcgaccga aggtccatac accgagctcg acggtctcac gatccagcgc
      901 gactcgcgct tcccggtccg tgtgactttg cagttctatc aagcgaccag caacggcgtg
      961 atctcgaaga ccaacgtcgc tgcgatggcc gcgcagatca agaaggtcta cggcaagggt
     1021 gactacgtcg gctcgctcgt cgtgccgacc gagctcgatc gcctgcgccc gacgaactgg
     1081 gtcggcgtga ccgaagcacc gctcgacgtg tggaaccacc tgcccggcct cctcgagcgg
     1141 atcggccagt atggctggcg ccgtccgcag atgcgcgccg gcgtgatgtg atcgattagt
     1201 cgtcgtcgag cgttcgcaag aacgcgacga ggtcgttgat ctcttgcggc gtgagcttgc
     1261 caccggttcg cacgcgctgc gcattcgtcg cgacctcggg ctcgggccat ttgcacgccc
     1321 cgacgcgcct gcaggagccg ctgatgaatt cgacgaattc gtcgaggcga tggatgtagc
     1381 cgttgtggcc gtacggcgac gtcttgccga cgttgcgcag cgtcggcgta cggaacatgc
     1441 cgtcgtgcgc tggatcgttc gtcgtcgtca tcaggccgtg atcgatgaat gcctcgccgt
     1501 cgggattgag atccttcgct agcccgtaga acggactgtc ggcgaacttc gggaggccga
     1561 ggttttcata cgtatacgtc gtgaacagcg gcggcgagcc gtcggcacct ggccggttcg
     1621 gatgacagct cgcgcagttt cccttcgccg gatcctcgaa caccgcgagg ccgcgcgctt
     1681 cgctgtcggt gagctgcgcg gtgcccgcga ggtacttgtc gtacttcgac gagaacggcg
     1741 ccatctgcgg cgtgcgctcg aacgcggcga tcgcatcacc gacgagcgcg aacgctttgt
     1801 cggtatcggc gaacgcgtcc ttgccgtaga gctggcggaa ctgcgcgccg tacgccttct
     1861 tcaccttcgc gacgaccgct gctttgctcg gattgttcat ctcgagcggg ttgacgagcg
     1921 gaaatgctgc ctgctcctcg agcgtgttcg cgcgaccgtc ccagaactga ccgccgagca
     1981 gcttaccgct cgcgtccttg tgcagcggcg gcgtgaattg cgagtagagc accgtcggcg
     2041 agttgcgcgt accgacgcgc tcgcgcagca cacccggcga cacgcgctcg aactcgggat
     2101 cgacgaacgt tgcctttgga tcgtggcagt cggcgcacgc ttggcctgcg ggctcggaca
     2161 gcttcgggtc gttgaacagc tgctcgccga gctcggcctt cgtgacgggt ttggggtttt
     2221 ccgccgacga cgcacagccg acgacgcacg cgacagcggc caaccacctc atcgagtagt
     2281 cagtgtagga ccacgcccga tgctcgcaac cgagctactt gatgacagcc aagcgcggag
     2341 accgcttcgg cttcgacaac acgctcttcg cgaccgcaag cgcggccttc gtggcgaggt
     2401 ccatgaacag cttcgcgttc tcggagtcgg ggctcgcgat gatcacgggt acgccggcgt
     2461 cgccgccgaa gcgaatccgc gtgtcgatcg gaatttctcc gaagaactcg gtgccgactt
     2521 cctgcgcgag gcgcttgccg ccaccgtggc tgaagatctc gtcgtggtga ccgcaggccg
     2581 ggcacttgta gtagctcatg ttctcgacga tgccgaggat cggaatctcg accttctcga
     2641 acatcgcgat gcccttcacg acatcgacga tcgacacctc ttgcggcgtc gtcaccatta
     2701 ccgcgcccgc gatcggaatc agctgcgaga gcgacagctg cacgtcgccg gtgccgggcg
     2761 gcagatcgca gacgagatag tcgagctcgc cccagtcgac gtcgccgagg aactgctgga
     2821 gcaggcgatg caccatcggg ccgcgccaca cgacggcctc gtccttgtcg acgaagaagc
     2881 cgaccgagat caccttcaca ccgtgatgga tcgccggaat gatccgcgac tccttcgacg
     2941 cctgcggcgg cacttcgggc gagccgagca tcgtcgggat cgatgggccg aatacgtcgg
     3001 cgtcgagcag gccgaccttc gcgccgtggc gctgcagcgc gagcgcgagg ttgaccgcga
     3061 ccgtcgactt gccgacgccg cccttaccgg cggccaccgc gatcacgttc ttcgggcctt
     3121 tgagcacggc cttgtcgctc ggcgccggca tgaacgccgt gttgacctcg ggaatgatcg
     3181 tcgtgcgctt ggcaccgagc gccttcaccg cggtgtcgac acgcgcgatg agctccttgc
     3241 gcagcgcgag tggatacgcg gcggtcggaa tgtcgagccg cacgagcacg tcgtcgccgg
     3301 agacttcgac cttgctcagg atgcggtacg aaacgatgtc cttgtcgaac gcgggatcgt
     3361 tgaccttcgc gagtgcttct cgaacttgtg cttccgtgat cgccatcgac ttcgtctccc
     3421 ggctcagggc aggtggccca gcttgagctt cttcgcgttc agatagtctg cgttgtgctc
     3481 ggactcgccg acccagtgcg cttcgagcgc gtcgacaatg acgccggcat gctcgagccc
     3541 cgccgccttc ttggggttgt tcgtcatcag ccggacgctc gtcacgccga gatcgcgcag
     3601 gatgccggcc gcgaggtcgt agctacgcaa gtcggcgtcg aacccgagcg cgaggtttgc
     3661 ctcgaccgtg tcgtgaccgt tgtcttgcaa ctggtacgcg cggaccttgt tgccaaggcc
     3721 aatgccgcga ccctcctgca ccaggtacac gagcacgccg cggccttcac gggcgatccg
     3781 cgcgagcgca ccctcgagct gagcccggca gtcgcagcgc aggctgccca tgacttcgcc
     3841 ggtgaaacac gacgagtgca cgcgggtcag cacggcctgg cccgtcacat cgcccaagac
     3901 catcgcgaca tgttcttcgt tctcgagccc aaccgcgctc gcccctgccc cacctggctc
     3961 gccggtgcgg tagaccacga ccctaaactc gccatgtggc gtcggcagtc gtgcgtcgga
     4021 atacctcgta agcatgcggg ttcggcgtgt atggccgggt cgcaagggct tgtcaatctg
     4081 ctaaggtggt ggggtcatgg cggttcgtcc cacggtgatt tggcccgatc ctaggttgcg
     4141 tgaaccaacc gtagagatca cgaccatcga cgacgccgtg cgcgagcttt accgagacct
     4201 ctgcgattcc atgtacgcgg aaaatggcct cggaatggcc gcgccgcagc tcggcgaggc
     4261 gcgcaagatg ttcatcgtcg agcccaagct cgccggcctc gccgaaacgg atccgccgct
     4321 gtgcttcatg aaccctgagg tgctcgaaac ctcggaagag atgcaggaca gcgaagaggg
     4381 gtgcctgtca ttccccgaca tctacatcaa agtgaagcgg ccgatgcgct gcaaggtccg
     4441 cgcacttggc atcgatggaa agcagttcac catcgaaggt gaaggcctgc tcgcccgctg
     4501 cctgctccac gaaaacgatc acttgaccgg caagctgctc gtcgatttcg tcggcccgct
     4561 gaaacggcag atgatcaaga agacgctgca gcgtcacaaa gacgccgaag ccgaagagct
     4621 cgaagaagcc gaagcctagg gcgcctgcga cggcaccgcg tactcttcgc tgctacttcc
     4681 cgcgaacggc ttcgggctcc agatcacgcg ctggatctcg acgccgtcga gcacacgtaa
     4741 tgcgcgcgcg tcggaaccga cgagcccttc gcagctgcgt ttgaagttca ccgcaacgtg
     4801 accgccgcgg cgatagcacg tcgggttgtc gccccagcac tcgcagtcgg tgagcgtcgc
     4861 cgcgccttgg aacgattgcc cgacgcgcca gtcgtggccg aaggtctcga cgcgcgggct
     4921 cgtcgcgtgc acgttgaaca gcgagccatc gctctcgttc gggatcaccc acgcgagctt
     4981 ctcgtcgccg agatataccg aatacgacag ccccgagtcg ttcgcagcgc gcacgtcgta
     5041 gccggcgaac gcggagcgaa gtgcggtcaa cgtcgccttc gacccggcgg tcagcgggcc
     5101 gaatccatcc tcggtaatcg taagcaggcc gcgcttgctg ggctccgccc gattggaaat
     5161 cgtcgccggg ggcgctttcg tgtagcagcc cgcgagcacg acgacccaag ccagccgcat
     5221 ggtgtcgcga ttgtaacgca cgtgcggtat agccgccgcg atgaccgatt ctgtgttcgt
     5281 acgcggcctc gaattcgagg gcaatcacgg ctatacggcg gccgaacggc gcggcacgcg
     5341 caggtttcgc gtcaacctca ccctcgagct ctcgctcgca gcggcagcgg tctccgacaa
     5401 gatctcggac acgatcgatt actggaaggt cagcgaagca gtcgtcgcga tcggcaccaa
     5461 gtcgacgttc aagctgctcg aagcactcgc gggcgcgatc ggctcgcgca tccaagagct
     5521 ctatccgcgt gcggccgtcg agatcgagct cgagaagctc gcgccgccct gtcccggcgt
     5581 gcccgctgcg tgcggcgtga agctcgtctt ccccgcccgc cagagtgaat ctggcggtct
     5641 ccgcctcggg cccgcgaagg aggcgtgatg ttttcctacg cgacacccgt gcgcttcgcc
     5701 gatgtcgatc acgcgggtat cgtctactac ccgcgcttct ttcattactt tcacgtcgcg
     5761 ttcgaagagc tgtggcgcgc gcgaatcgga ccacaggcgt actccgacat catcgatcgc
     5821 gatcgcatcg gctttcctgc ggtgcgcgcc gagtgcgact tcaaagcacc cctgaaattc
     5881 ggagacaccg ccgagatcga cgtcacgatt ccgcgcctcg gcactaagtc gatcacgttc
     5941 agatatcgca tctatcgcgg agaggccgtg ccatccggca cacgcgtgct gtgcgctgaa
     6001 ggccaggtcg tgtgcgcggt ggtcgacctc gcgaaatttg ccgcggttgc gattccggag
     6061 catgtgacca acatgttgcg tgatttagtc gaatcgtgat gccctccacg cggcatagaa
     6121 aaagcagctt gctctttacg cccgctgggc gatcgtacta agtccccgtc atgatcgagc
     6181 gcgaccccaa gactctggcc aggctgcagg cggtggtaac cgccctcatc gagcaccgcg
     6241 tctcgacgtc cctggtcgat gtcgagcggg cgctcgcgaa atggcgcagc ggtcagcact
     6301 cggccctcgc cgcacacggc gcggtcctgc gccacgctgc gcgctgcgag cgcacggtcg
     6361 agcgcgtcac cgcagccgct ggtgaccgcc ccgaaggcat tctgcgtgac gcggtcgatg
     6421 ccggcctgat gtcgagcgaa gaattcgtgc agcttgtcgg caaacagccg aacgaagtcc
     6481 cctcgatcga cgcgctccgc gacgaagacg acgcctcagc ccctgacaag cgccgcacgc
     6541 tcgagacgct gctcgcgcgc ggccccgtgc tcgtccatgt cgatgcgcgc cgcgccgaag
     6601 tctcggtccc gccgcgtttc cgcgccgatc cgagcctcgt gctccgcttc ggctacacgc
     6661 tatcgccggc gatcgcggat ctcggcgtcg acgacaacgc gatctccggc acgctcacgt
     6721 ttggcggcca gccgttccac tgcgtgctgc cgtggaccgc ggtctacgcc gcgatggtcg
     6781 aaggcgagca gcgcggcacc gtgtggccgg aagacgtccc cgaagacgtg ctcaccggcg
     6841 gcggcgaagc cccggctccg ctgccggccg cgcagccgca acagcaagcc gagccgcagc
     6901 cgcagcgcgc caagcgcacg agccacctga agctcgtcga gtaacgcatg cgcgcgatcg
     6961 tcgcgctcgc actgctcgcc gcatgcgcac cgggcaagcc cgagaagctg cgcagcccag
     7021 gcggtggtgg ccaccgtcca ccgggcgggc caccgaaccc gccgcacgtc gcgtggttcg
     7081 gtcgtttcga gctcac
//
LOCUS       DASUFX010000112         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1825118, whole genome shotgun sequence.
ACCESSION   DASUFX010000112 DASUFX010000000
VERSION     DASUFX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825118"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..2111)
                     /gene="thrS"
                     /locus_tag="VFV99_04295"
     CDS             complement(147..2111)
                     /gene="thrS"
                     /locus_tag="VFV99_04295"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5058554.1"
                     /translation="MSEPNVTITLPDGSTKQVAAGTTISDFVRTQIGTGLAKAALFAK
                     YDGAEVDLTHKLDHDGALAIFTTKNPEALDLVRHDAAHIVASVVQRLFPGTQVTIGPT
                     IEDGFYYDFYREKPFTEEDLKTIEAAANEEIKKDLPFHRKEVSKDEAIALFTRLGEKF
                     KLELVEDIFNKGAPTLSLYEHGDWVDFCLGPHGPSTGRVGVIKILSVAGAYWRGDHRN
                     PQLQRIYGTAFFTQKDLDAWTHQQEEAKKRDHKRLGPALDLFHFHPAAPGAAFWTAKG
                     TVIYTTLQTAMRNMCLANGYQEIKTPLIYNKSLWETSGHWGKYRENMFLIFDKESDEK
                     LPAEERASSSLKPMNCPSHYLFYQIKRRSYRELPLRLHTQDVLHRNEAAGALSGLTRV
                     RQFQQDDAHIMLMESQIADEVAALTKLISRVYSAFGLEFTAKFATRPEVKIGDDALWD
                     KAESALKAALIATGLPYELKPGDGAFYGPKIDFDVADSIGRKWQLGTIQLDYNAPERF
                     DMTYIGDDNKEHRPVVIHRAIYGSFERFIGILIEHFAGNVPVWLAPEQARILPVAERH
                     HAWADEVAAKMRAAGLRVDVDRSHGRIGGMIRESEIAKVPYALVVGDKEVEAQGVSPR
                     KHGAGKEGDLGLMPLDQFVARLTDEAKIPY"
     gene            2364..>3390
                     /locus_tag="VFV99_04300"
     CDS             2364..>3390
                     /locus_tag="VFV99_04300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058555.1"
                     /translation="MIQVGQVLDKYELLERVGQGGMAIVYRGIDRSLKRTVAIKVLHK
                     HLSDYQEARDRFEREAQAVAKLRHENILEIFDYSAKADSESYIVTEFIDGQTLKQFFT
                     DRPIGYPEVGAMLMLQVARALAHAHAIGILHRDIKPENIMIRSDGVVKLMDFGISHMV
                     DLERLTVTGQLLGSPAYMAPEHVEGRQLDFRTDVFAVGIVLYQLTVGRLPFEGKNPHE
                     VLKRIAECRFVDPRQANPRIGNRLGKIILRAMAAQPADRYPAIGEMVTALEAYLDESG
                     LATDKVTSELARYFKAPASYEQALKERLVDHLTRRGQELLGNGEQAPALDVFDRVLTI
                     DPKNEKVL"
BASE COUNT          585 a         1093 c         1092 g          620 t
ORIGIN      
        1 gcggcttcca ctgggtgcaa gagcagccgt actaccgctg acatccacgc gcgcaccgcg
       61 cgccaccacg gcaacggcgc ttcagcgtgc gcgggctcga acggacgacc gagtcgcggc
      121 gtgagaagtc gcgtcgcgct cggagctcag tacggaatct tggcttcgtc ggtgagccgc
      181 gcgacgaact gatcgagcgg catcaggccg aggtcgcctt ccttgccggc gccgtgcttg
      241 cgcggcgata cgccctgcgc ctcgacttcc ttgtcaccga cgacaagcgc gtacggcacc
      301 ttcgcgatct cgctctcgcg gatcatgccg ccgatacgcc cgtgcgaacg atcgacgtcg
      361 acgcgcaggc cggccgcgcg catcttcgcg gcgacctcgt cggcccacgc gtgatgacgc
      421 tcggccaccg gcaggatgcg cgcctgctcg ggcgcgagcc acaccgggac gttgccggcg
      481 aagtgctcga tcaagatgcc gatgaagcgc tcgaacgagc cgtagatcgc gcgatggatg
      541 acgaccgggc gatgctcctt gttgtcatcg ccgatgtacg tcatgtcgaa gcgttcgggc
      601 gcgttgtagt cgagctggat cgtgccgagc tgccacttgc gaccgatcga gtccgcgaca
      661 tcgaaatcga tcttcggacc gtagaacgcg ccgtcgccgg gcttgagctc gtacggcaga
      721 ccggtcgcga tgagcgcagc cttgagcgcc gactctgctt tgtcccacag cgcgtcgtca
      781 ccgatcttca cctcgggacg cgtcgcgaac ttcgcagtga actcgagacc gaacgccgag
      841 tacacgcgcg agatcagctt cgtcagcgcg gcaacctcgt cggcgatctg cgactccatc
      901 agcatgatgt gcgcgtcgtc ttgctggaac tggcgcacgc gcgtcaggcc gctgagcgca
      961 cccgccgctt cattgcgatg gagcacgtcc tgcgtgtgca gacgcagcgg cagctcgcga
     1021 tagctgcggc gcttgatttg atagaacaag tagtgcgacg ggcagttcat cggcttgagc
     1081 gatgagctcg cgcgctcctc ggcggggagc ttctcgtcgc tctccttgtc gaagatgagg
     1141 aacatgttct cgcggtactt gccccagtgg ccgctcgtct cccacaacga cttgttgtag
     1201 atgagcggcg tcttgatctc ctggtagccg ttcgcgaggc acatgttgcg catcgcggtc
     1261 tgcagcgtcg tgtagatgac ggtgcccttc gcggtccaga acgctgcgcc cggcgcggcc
     1321 gggtggaagt ggaagagatc gagcgcgggg ccgagccgct tgtggtcgcg cttcttcgct
     1381 tcctcctgct ggtgcgtcca cgcgtcgaga tccttctgtg tgaagaacgc ggtgccgtag
     1441 atgcgctgca gctgcgggtt gcgatgatcg ccgcgccagt acgcgccggc gaccgagagg
     1501 atcttgatca cgccgacgcg cccggtcgac ggaccgtgcg gaccgaggca gaagtcgacc
     1561 cagtcgccgt gctcgtagag cgacagcgtg ggcgcgcctt tgttgaagat gtcctcgacg
     1621 agctcgagct tgaacttctc gccgagccgc gtgaacagcg cgatcgcctc gtccttgctc
     1681 acctctttgc gatggaatgg gaggtccttc ttgatctcct cgttcgccgc ggcctcgatc
     1741 gtcttgaggt cttcctcggt gaacggcttc tcgcggtaaa agtcgtaata gaagccgtcc
     1801 tcgatggtcg ggccgatggt cacctgcgta ccggggaaca gccgctggac gaccgacgcg
     1861 acgatatgtg ccgcatcgtg ccgcaccaga tcgagcgcct ccgggttctt cgtggtgaat
     1921 atcgcgagcg cgccgtcgtg atcgagcttg tgggtcagat cgacctcggc accgtcgtac
     1981 ttcgcgaaca atgcagcctt tgccaggccg gtcccgatct gcgtgcggac gaagtcgctg
     2041 atcgtcgtgc cggcagccac ttgcttggtg gagccatcgg gcaaggtgat cgtgacgttc
     2101 ggttcactca tgggcggcgc agcctacccc gagttgccta ccgccgcagg ttcccagcga
     2161 tcgggatcca tgaggaaatg cggacgaacc ccgatgacgc ccacgtcaga ccctcgcagc
     2221 gctcccgcct tgctgaatct tctagaagcg gcgtagactt ggcaccttag acaaccttat
     2281 gagactgccc gcggtcgtct catttcggca tgaacgcatg gatggccccg tgacatctac
     2341 gtcatggcgg tggagcgaac ggattgatcc aggttggtca ggttctcgac aagtacgagc
     2401 tgctggagcg cgtgggccag ggcgggatgg cgatcgtcta tcgaggcatc gatcgatcac
     2461 tgaagcgcac cgtcgcgatc aaggtcctgc acaagcacct gtctgactac caggaggcac
     2521 gcgaccgctt cgagcgcgag gcccaggctg tcgcgaagct gcgccacgag aacatcctcg
     2581 agatcttcga ctactcggcg aaggcggact ccgagagcta catcgtcacc gagttcatcg
     2641 acgggcagac gctcaagcag ttcttcaccg accggccgat cggctatccc gaggtcggcg
     2701 ccatgctcat gctacaggtc gcgcgcgcgc tcgctcacgc gcacgccatc ggcatcctgc
     2761 accgcgacat caagccggaa aacatcatga tccggtccga cggtgtcgtg aagctgatgg
     2821 acttcggcat ctcgcacatg gtcgacctcg agcggctcac cgtcacgggc cagttgctcg
     2881 gctcgccggc gtacatggcg cccgagcacg tcgaaggccg acagctcgac tttcgcaccg
     2941 acgtgttcgc cgtcggcatc gttctctatc agctcaccgt cggcagattg cccttcgagg
     3001 gcaagaaccc gcacgaggtg ctcaagcgca tcgccgagtg caggttcgtc gatccacggc
     3061 aagcgaatcc gcgcatcggc aaccggctcg ggaagatcat cttgcgcgcg atggccgcgc
     3121 agccggccga tcgatacccg gcgatcggcg agatggtgac cgcactcgag gcgtatctcg
     3181 acgagtcggg gctcgcgacc gacaaggtga cgagcgagct cgcgcgctac ttcaaggcgc
     3241 cggcgtcgta cgagcaggcg ctcaaggagc ggctcgtcga tcacctcacg cggcgcggcc
     3301 aggagctgct tggcaacggc gagcaagcgc ccgcactcga cgtgttcgat cgcgtgctga
     3361 cgatcgatcc gaagaacgag aaggtgctcg
//
LOCUS       DASUFX010000113        11895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1685757, whole genome shotgun sequence.
ACCESSION   DASUFX010000113 DASUFX010000000
VERSION     DASUFX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685757"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFV99_04305"
     CDS             complement(<1..852)
                     /locus_tag="VFV99_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5058556.1"
                     /translation="MVATASGFLYEHLADELGQAIDRGALRAGDRLPSVRRLAQERSV
                     SVSTVLEAYLQLENAGLIEVRPKSGHFVRRRAPLTAEPRTPRVCQKPSRVTVGDAYTK
                     ILEAMRDPELVPFGCATIDPEYLPLAQLNRIVSQLSREMTTLGARYEAAPGLLTLRRQ
                     VARRAVEWGVALSENDVCTTIGATEAISLALRAVAKPGDVIAVESPAYFGVLQAIESL
                     GLRALEIPAHPRTGFDVSAFEEAIRAQPVRAVFVSPTVSNPLGAVMPEDERERLVKIT
                     RRHDIPII"
     gene            991..1899
                     /locus_tag="VFV99_04310"
     CDS             991..1899
                     /locus_tag="VFV99_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5058557.1"
                     /translation="MITSTTPDQLADKPSVDPRLIASLASVYIIWSSTYLAMRIVVVE
                     LPPLFSAAIRFTLAGTVMMVMALRRGARLPSLRDWLRVAPAGLLLFVGGNGFVSIASQ
                     SLTSGGVAVVCATMPLWIGVLGYAIGERPTAREWLSLLIGFAGVLVLMGSPSLAGKPL
                     HIALVIGSPILWAAGSLLARRTKDVGGVHGGLVGPAVQMLTGGAALAVIALVRGERMP
                     LDAHVSSYLALAYLCVFGSLVAFTAYAWLLRNARPVVATSYAYVNPILAVLIGAALYG
                     EPLSWTTALANILIVGAVMLALTRRR"
     gene            1937..3133
                     /locus_tag="VFV99_04315"
     CDS             1937..3133
                     /locus_tag="VFV99_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058558.1"
                     /translation="MLRVVLRLLLATAVVGGVTTACSGGSKTVKHAKKGKKPKNNDAR
                     ALVSEARDDAKNGEYDAADKAYADAYEMSKDFDVLEERVDFLIHSGRATKAVDAAKAY
                     YDANVSDNKGYALYAEALLAASKGTEALEVADQLVQLNGDDPAGHEKKGRALILLEKN
                     EEGLDELRKATQLDQTSATYHRSLGLALNKLGKIDESALEFRAAIKAAPDDAEAHVLL
                     GMALRGQQEYDEAKSYLDKAIELDPRNGRAYFELGLLYNAQKKQADSEIAFSKAVQLA
                     PNESLFWYAYGEIYRVQERFDEAISAYKKAVEIDPPYPKALAKLGLLLVERKQYDDAE
                     MLLTQAVRREPKAAPAYFTLGQLYEAKKKNKLAIENYQKFLDLAPKTDPDREHASAAI
                     KALKRK"
     gene            3208..3894
                     /locus_tag="VFV99_04320"
     CDS             3208..3894
                     /locus_tag="VFV99_04320"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5058559.1"
                     /translation="MVDIGAASRALEGEPPLRILQWASMQIPRLTFATGFGAEGCVII
                     DLIGTHELPIDIFTLDTGLLFPETYALWKQLEAKYGITIRGVTPAQTVDEQAVTHGPA
                     LWERVPDRCCDLRKVQPLTRALSGFAAWVTAIRRDQTEERANAQIVESDHKFGLIKIN
                     PLVAWTHDDVWGHLYANDVPYNVLHERGYPSIGCQPCTSQVVPGENLRAGRWRGTGKN
                     ECGLHVKEKP"
     gene            3891..4475
                     /locus_tag="VFV99_04325"
     CDS             3891..4475
                     /locus_tag="VFV99_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX5058560.1"
                     /translation="MTAALYPAFLKLEGLPVVVVGGGTVAASKLDGLLAAGAHVTVVA
                     PHVVPSIRERVAVTVAVTVIEQAFRPEHLSGARWVVAAAPPAVNREVATAAAARGLFV
                     NAVDDPSVATAYLGGVVRRGPVEIAISTGGLAPALAGLLREALEAILPDDLDKWIEIA
                     KSERAAWKQARVPMPERRPLLLRALERLYSRGAA"
     gene            4472..5230
                     /gene="cobA"
                     /locus_tag="VFV99_04330"
     CDS             4472..5230
                     /gene="cobA"
                     /locus_tag="VFV99_04330"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5058561.1"
                     /translation="MSGFVSLVGAGPGDPDLLTLRAVDRLRRADLVLYDALVEEELLT
                     LAPQAKRFYVGKRAGRHSIDQDGIHRLMVRAAQRGERVVRLKCGDPFVLGRGGEEALA
                     LEAAGVPYEVVPGLSSAVAAPALAGIPVTHRGLAAGFAVLSGHAAASYGPALDSIAPG
                     SLTLVVLMGLRTRGEIAERLVQRGWASDTPAAIVLGASHAGSTRWIGTLATLASADID
                     SELAGVIVVGAVVELADKIANDYAAVPAAALENA"
     gene            5254..5544
                     /locus_tag="VFV99_04335"
     CDS             5254..5544
                     /locus_tag="VFV99_04335"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEX5058562.1"
                     /translation="MTGKFTEDAANAWRRADGTWSRQLAEAGLYTEEAAAKAQVVDTV
                     AKEQREITDPYVIEVYAANGTIDPLTARERIRATGPTIRIRRPDPAPKADRG"
     gene            5546..7234
                     /locus_tag="VFV99_04340"
     CDS             5546..7234
                     /locus_tag="VFV99_04340"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5058563.1"
                     /translation="MYQYDDYDRRIITERAQQFRGQVARRLSGEITEEEFKPLRLQNG
                     LYAQLHAYMLRVAIPYGLLSSTQVRKLAHIARTYDKGYGHLTTRQNIQYNWPKLADVP
                     TILDELATVEMHAVQTSGNCVRNITADHFAGIAPDELEDPRPYCEILRQWSTFHPEFA
                     ALPRKFKIAITGAPGEDRAAVRFHDIGLRIVENADGERGFEVLVGGGLGRTPEIGRVI
                     REFLPKKHFLSYMDSILRVYNLFGRRDNKFKARIKILVTALGVEEFRRRVEEDWEASR
                     TPELELPDEEIARIAAFFTPPPYEKLPANDTQVAGITLGRDRELGNWVRNNVMRHKVP
                     GYSAVVVSVKVPGTPPGDVTDTQLDAIADVADRYSFGRIVITHTQNFVLSDVKTSDLP
                     RLHAELVALDLGKSNVNKLTDVITCPGLDFCDLANARSIPLSLEIAERFDRIDYLNDI
                     GDVSIKMSGCINACGHHHVGNIGILGIDKRGEEWYQLMLGGSPGNDASIGQILGAALA
                     QDEIVDAVERVIERYLEIRESADERFIETVRRVGLDPFKVAVYGAPRTALAAGA"
     gene            7231..7713
                     /locus_tag="VFV99_04345"
     CDS             7231..7713
                     /locus_tag="VFV99_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX5058564.1"
                     /translation="MTMQIIRNRTIVDDDFVHVPDGAELPDGKPIVTLARYLAAREEL
                     LARYPALGVRIASDKLPTDIPELTRLALIAIEFPRFTDGRGYSVARMLRERHKFTGEL
                     RAVGWVLRDNLFYMERCGFTAFELKPGKPLNSALEAFGELSVTYQAATDEKRPIYRRR
                     "
     gene            complement(8071..8376)
                     /locus_tag="VFV99_04350"
     CDS             complement(8071..8376)
                     /locus_tag="VFV99_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058565.1"
                     /translation="MSRVVALVACVALLGGCFGYNSSAKKWAYAGNTVLILGGGGAIA
                     ADLLTHEKCMSTATIGDNCPAFDPPFGGGTVVGAVLVTAGIVGMVINATRAEVKTSR"
     gene            8437..9867
                     /locus_tag="VFV99_04355"
     CDS             8437..9867
                     /locus_tag="VFV99_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5058566.1"
                     /translation="MPQRSPLLPIFLIVLVDVLGFTIVIPLLGFYAEKFGASPLVATT
                     LVSVYALCSLISTPIIGNLSDRYGRRRMLLISQAGTLAGFLMLANSSALWMVFAGRIL
                     DGTTAGNLSLAQAYISDHTAPENRAKAFGVIGVAFGVGFMFGPGIGGVLAEYGMHVPF
                     IAAASLSLLSIIATYTLLGKETPPQKTQDGPLPGGKRPGAFDVSTYSEYFRRPMLGRL
                     YLQFFMFTFAFSCFTSGFALFSERRFTAEHEIHRVESSCVLELDTRIEMPGAEFGTIR
                     LDGRALAYGRDWIVFGPVIVLTHAEDCARLDSAKLSARWPWTAHEVGLLFMFSGFLGI
                     LLQGGLIGRLVKRYGEPKLIVAGFAAASVAYVALGLTFTLSLLLVVAVLMSFGNGVLR
                     PVITSRITQVAGRHEQGTAIGISGSLSSFAMMLAPPTGGSLINGHHLLGWALIPATVT
                     AIGLIIASLPAKTAGTKQDPELSSPP"
     gene            9864..10370
                     /locus_tag="VFV99_04360"
     CDS             9864..10370
                     /locus_tag="VFV99_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058567.1"
                     /translation="MKTLGWMATALVAGGVAFTIKGGCLSSEKAPPDARLAGHFEKLM
                     CGIARDNIKTPEKGVRELGRYMDKHAGHMFGDFGDMLATIERIPDDAKHDRRAEKARD
                     RLAKPAIACGRDWNRFGEAISDDPKAMELLERFNERLGRTFEIIFGSGQHFDVLQLPQ
                     QMLGTLEK"
     gene            10487..11218
                     /locus_tag="VFV99_04365"
     CDS             10487..11218
                     /locus_tag="VFV99_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058568.1"
                     /translation="MRNILCALVCLLAIPVVAACSHASPKPAAPTPATVATAEPERFD
                     DKVRADFFDGMRGDEAAMARAMKVCDDALAKNPKNAEAMVWHGAGVVARARTAFAAGN
                     RDQGIALYTQGIGEMDAAVALEPNNVGVRIPRGAVFLAMAPFVPEPEKTKLLEHGLDD
                     YETTYALQQKAGYFPKLTLHAREQLLYGLTDGYANLGKADKAAAYVEKMKSEAAGSEL
                     LPRAASRARGEAANGPAPCEQCHRR"
     gene            11228..>11895
                     /locus_tag="VFV99_04370"
     CDS             11228..>11895
                     /locus_tag="VFV99_04370"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5058569.1"
                     /translation="MAEPLRLRVTRAALLSVGLSVVIGFLFSLHNLTAIRFALNTAVS
                     LLYSLSIGVPATLIFDRLQPRIGRPVLRQWAIYVGVMLAIVLVASLFVGVVLVLVGLI
                     ESSMLWGVFRQGLVVSLVISIPVTLAVITVSRLYEKLGDTERDKARALASAAEARLAS
                     LESRVRPHFLFNALNSAIALIPDSPRRAEDVLERLSALLRSSLDAQARLVPLGDELRV
                     ATDYL"
BASE COUNT         2021 a         3998 c         3861 g         2015 t
ORIGIN      
        1 gatgatcggg atgtcgtggc ggcgcgtgat cttgacgaga cgctcgcgct cgtcctccgg
       61 catcaccgcg ccgagcgggt tcgacaccgt cggcgatacg aagacggcgc gcaccggttg
      121 cgcgcggatc gcttcttcga acgccgacac gtcgaagccc gtgcgcggat gcgccggaat
      181 ctcgagcgcg cgcaggccga gactctcgat cgcctggagc acgccgaagt acgcaggtga
      241 ctcgaccgcg atcacgtcgc cgggcttggc gaccgcgcgc agcgcgagcg agatcgcttc
      301 cgtcgcgccg atcgtcgtac acacgtcgtt ctccgacaac gccacgcccc actcgacagc
      361 acggcgtgcg acctgacggc gcaacgtgag cagacccggt gcggcttcgt agcgagcacc
      421 gagcgtcgtc atctcgcgcg agagctggga aacgatccga ttgagctgcg cgagcggtaa
      481 gtattcggga tcgatcgtgg cgcacccgaa tggcacgagc tcgggatcgc gcatcgcttc
      541 gaggatcttc gtgtacgcat ctccgacggt gacacgcgac ggcttctggc acacgcgcgg
      601 cgtgcgcggt tctgccgtca gcggcgcgcg ccggcgcacg aagtggccgg atttcgggcg
      661 cacctcgatc agacctgcgt tctcgagctg cagatacgct tcgagcacgg tcgacacgct
      721 cacgctgcgc tcctgcgcga gccggcgtac cgacggcagc cgatctccgg cgcgcaacgc
      781 accgcgatcg attgcctggc ccagctcgtc tgcgaggtgc tcgtaaagaa acccgctcgc
      841 tgttgccacc atgcgcaaaa gtgtacccat cgtattccca ttgaaccagt acagatgccg
      901 aggccagtag cggtacagga gatctgtgct ggtgatcgcg cgaatttcgt gtagctgtac
      961 tgctcgcgat cgcgcgtcat catgaggccc gtgattacat cgacgacccc cgatcagctc
     1021 gctgacaagc catccgtgga tccgcgcctg atcgcgagcc tcgcgtcggt ctacatcatc
     1081 tggagctcga cgtacctcgc gatgcgtatc gtggtcgtcg agctaccacc gctgttctcg
     1141 gcggcaatcc gcttcacgct cgccggcacc gtgatgatgg tgatggcgct tcgccgcggc
     1201 gcgcggctgc cgtcgctgcg cgattggctg cgcgtggcgc cggccggcct cctgctgttc
     1261 gtcggtggca atggcttcgt ctcgatcgca tcgcagtcgc tgacgtccgg tggcgttgca
     1321 gttgtctgcg cgacgatgcc gctgtggatc ggcgtgctcg gctatgcgat cggcgagcgg
     1381 ccgaccgcgc gcgagtggct gtcgctactg atcggctttg ccggcgtgct cgtgctgatg
     1441 ggcagcccgt cgttggcggg caagccgctc cacatcgcgc tcgtgatcgg atcgcccatc
     1501 ttgtgggcgg caggctcgct gctcgcgcgt cgcacgaagg atgtcggcgg tgtccatggc
     1561 gggctcgtcg gccctgccgt gcagatgctc accggcggcg cagcgcttgc ggtgatcgcg
     1621 ctcgtgcgtg gcgagcgaat gccactcgac gcccacgtgt cgtcgtacct cgccctcgcc
     1681 tatctctgcg tcttcggctc gctggtcgcg tttaccgcct acgcgtggct gttgcgcaac
     1741 gcgcggcccg tcgtggccac gagctacgcg tatgtgaatc cgatcctggc cgtcctgatc
     1801 ggcgcggcgc tctacggcga accgctgagc tggaccacgg ccctcgccaa tatcctgatt
     1861 gtcggcgcag tgatgcttgc actgaccaga cgccggtgaa gtggcgcgcg cccggggctt
     1921 tcgctacgct caataaatgc tccgggtggt ccttcgcctc ttgctcgcca cggccgtcgt
     1981 cggcggggtc acgaccgcgt gcagcggcgg cagcaagacc gtcaagcatg ccaagaaggg
     2041 caagaagccg aagaacaacg acgctcgcgc gctcgtctcc gaggcacgcg acgacgcgaa
     2101 gaatggcgag tacgacgcgg ccgacaaggc ctacgccgat gcgtacgaga tgtcgaagga
     2161 cttcgacgtc ctcgaagagc gcgtcgactt cttgatccac tccggccgcg cgacgaaggc
     2221 tgtcgacgcc gcgaaggcgt attacgacgc gaacgtcagc gacaacaaag gctacgcgct
     2281 ctacgccgaa gcactgctcg cggcgagcaa aggcaccgag gcgctcgagg ttgcggatca
     2341 gctcgtccag ctcaacggcg atgatcccgc gggccacgag aagaagggcc gcgcgctgat
     2401 cctgctcgag aagaacgagg agggtctcga cgagcttcgc aaggcgacgc agctcgatca
     2461 gacgagcgcg acctatcacc gctcgctcgg tctcgcgctg aacaagctcg gcaagatcga
     2521 cgagtcggcg ctcgagttcc gcgccgcgat caaggccgcg ccggatgacg ccgaagcgca
     2581 cgtgctgctc ggcatggcgc tgcgtggcca gcaggagtac gacgaggcga agagctacct
     2641 cgacaaggcg atcgagctcg atccacgcaa cggtcgcgcg tacttcgagc tcggcctgct
     2701 ctacaacgcg cagaagaaac aagccgactc cgagatcgcg ttctccaaag cggtccagct
     2761 ggcgccaaac gaatcgctgt tctggtacgc gtacggcgag atctatcgcg tgcaggagcg
     2821 cttcgacgaa gcgatcagcg cgtacaagaa ggccgtcgag atcgatccgc cctatcccaa
     2881 ggcgctcgcc aagctcggcc tgctgctcgt ggagcggaag cagtacgacg acgccgagat
     2941 gctcctgacc caggccgtcc gccgcgagcc caaggccgcg cccgcctact tcacgctcgg
     3001 ccagctctac gaagccaaga agaagaacaa gctcgctatc gagaattacc agaaattcct
     3061 ggatcttgcg ccgaagaccg accccgatcg ggagcacgcg agcgcggcga tcaaagcgct
     3121 gaagcggaag tagcctagcg gcccatccgc tatcgttgac agtctgtccg ctgagggtat
     3181 agacatatat ccgttatgac ggacgtcgtg gtcgacatcg gagccgcctc gcgcgctctg
     3241 gaaggcgagc cgccgctccg cattctgcag tgggcaagca tgcagatccc acggctgacg
     3301 ttcgcgaccg ggttcggcgc cgaaggctgc gtgatcatcg acctgatcgg cacgcacgag
     3361 ctgccgatcg acatcttcac gctcgatacg ggtctgttgt ttccggagac ctacgcgctg
     3421 tggaagcagc tcgaagcgaa gtacggcatc acgatccgcg gcgtcacgcc cgcgcaaacg
     3481 gttgacgagc aagcagtgac gcacggcccg gcgctgtggg agcgcgtgcc tgatcgctgc
     3541 tgcgatctgc gcaaggtgca gccgctgacg cgcgcgctgt ctgggttcgc ggcatgggtc
     3601 accgcaattc gccgcgatca aaccgaagaa cgcgcgaacg cgcagatcgt cgagagcgat
     3661 cacaagttcg gtttgatcaa gatcaatcca ctcgtcgcgt ggacgcacga cgatgtgtgg
     3721 ggccacctct acgcgaacga cgtcccgtac aacgtgctcc acgagcgcgg ctatccgtcg
     3781 atcggctgcc agccgtgcac gtcacaggtc gtgcccggcg agaacctgcg cgcagggcgg
     3841 tggcgcggca ctggcaagaa cgagtgcgga ctgcacgtga aggagaagcc atgaccgctg
     3901 cgctctaccc ggcgtttctc aagctcgaag ggcttcccgt cgtggtcgtc ggtggcggaa
     3961 ccgtggctgc gagcaagctc gacggtctcc tcgccgcggg cgctcacgtc accgtcgtcg
     4021 cgccgcacgt cgtgcccagc attcgcgagc gcgtcgccgt gacggtggcc gtgacagtga
     4081 tcgagcaggc gtttcggccg gagcacttgt ccggcgcacg ctgggtcgtt gctgcggcgc
     4141 cgcccgcggt gaatcgcgag gtcgcgacgg cagctgccgc gcgcggtctg ttcgtcaacg
     4201 ccgtcgacga tccgagcgtg gcgactgcgt acctcggcgg cgtcgtgcgc cgcggtccgg
     4261 tcgagatcgc gatttcgacc ggcggcctcg cgcccgccct cgcaggccta ctgcgcgaag
     4321 cactcgaagc gatcctgccc gacgacctcg acaagtggat cgaaatcgcg aagtccgagc
     4381 gcgcggcgtg gaagcaagcg cgcgtgccga tgcccgagcg gcgcccgctg ctcttgcgcg
     4441 cactcgaacg cctctacagc cgaggtgcgg catgagcggc ttcgtctcgc tcgtcggcgc
     4501 cggccccggc gatccggatc tgctgacgct gcgcgcggtc gatcggctgc gccgcgccga
     4561 cctcgtgctc tacgacgcgc tcgtcgagga agagctgctc acgctcgcac cccaggcgaa
     4621 gcgcttctac gtcggcaagc gcgccggccg ccactcgatc gatcaagacg gcatccatcg
     4681 cttgatggtg cgcgccgccc agcgcggcga gcgcgtcgtc cgcctcaagt gcggcgatcc
     4741 gttcgtgctc ggccgcggcg gtgaagaagc gcttgcactc gaagcagcgg gcgtgccgta
     4801 cgaggtcgtg cccgggctgt cgtcggcggt tgccgcgccc gcgctcgccg gcattcccgt
     4861 cacgcatcgc gggctcgccg ccggtttcgc cgtgctgtca ggtcacgcgg cagcgagcta
     4921 cggccccgca ctcgattcga tcgcacctgg ctcgctgacg ctcgtcgtgc tgatgggcct
     4981 gcgcacgcgc ggcgagatcg ccgagcgcct cgtccaacgc ggctgggcga gcgatacgcc
     5041 ggcggcgatc gtgctcggtg cgtcgcacgc tggtagcacg cgctggatcg gcacgctcgc
     5101 gacacttgca agtgccgaca tcgatagcga gctggccggc gtgatcgtcg tcggcgcggt
     5161 cgtcgagctg gcggacaaga ttgcaaacga ctacgccgcg gtgcccgcgg ctgcactgga
     5221 gaacgcgtaa tgccgacggc gaatcggtgg atagtgaccg gaaagttcac ggaggacgca
     5281 gcgaacgcgt ggcggcgcgc tgatggtacg tggtcgcggc agcttgcaga ggcgggcttg
     5341 tacaccgagg aagctgcggc caaggcgcag gtcgtcgaca ccgtggcgaa ggagcagcgc
     5401 gagatcaccg atccctacgt gatcgaggtc tacgccgcga atggaaccat cgatccgttg
     5461 accgcacgcg agcggattcg cgcgacgggt ccaacgattc ggatacgccg accggatccc
     5521 gcgccgaagg cagacagagg ctgacatgta ccaatacgac gactacgacc gcaggatcat
     5581 cacggaacgt gcgcagcagt tccgtggcca agtcgcacgt cgcctgtcag gcgagatcac
     5641 cgaggaggaa ttcaaaccac tgcggctgca gaacggtttg tacgcgcagc tacacgccta
     5701 catgctgcgc gtcgcgatcc catacggact cttgagctcg acgcaggtcc gcaagctcgc
     5761 gcacatcgca cgcacgtacg acaagggcta cggccacctg acgacgcgcc agaacatcca
     5821 atacaactgg cccaagctcg ctgacgtgcc cacgatcctc gacgagctcg cgaccgtcga
     5881 gatgcacgcg gtgcagacga gcggcaactg cgttcgcaat atcaccgccg atcacttcgc
     5941 cggcatcgcc cccgacgagc tcgaggatcc gcgcccgtac tgcgagatcc tgcgccagtg
     6001 gtcgacgttc catcccgagt tcgcagcgct gccgcgcaag ttcaagatcg cgatcactgg
     6061 cgcgccgggt gaagaccgcg ccgcggtgcg cttccatgat atcggcctgc gcatcgtcga
     6121 gaacgcagac ggcgagcgtg gcttcgaagt gctcgtcggc ggtggcctgg gtcgcacgcc
     6181 ggagatcggc cgcgtgattc gcgagttcct cccgaagaag cacttcctgt cctacatgga
     6241 cagcatcttg cgcgtgtaca acttgttcgg tcgccgcgat aacaagttca aggcgcgcat
     6301 caagatcctg gtcaccgcgc tcggcgtcga agaattccgc cgtcgcgtcg aggaggactg
     6361 ggaagcgagc cgcacgcccg agctcgagct ccccgacgag gagatcgcgc gcatcgccgc
     6421 gttcttcacg cctccgccgt acgagaagct gcccgcaaac gacacgcagg tcgcgggcat
     6481 cacgcttggc cgcgatcgcg agctcggtaa ctgggtccgc aacaacgtca tgcggcacaa
     6541 ggtgccgggg tacagcgctg tcgtcgtctc ggtgaaagtg cctggcacgc cgccgggcga
     6601 cgtcaccgac acgcagctgg atgcgatcgc cgatgtcgcg gatcgctact cgttcggccg
     6661 catcgtcatc acgcacacgc agaacttcgt gctctcggac gtcaagacgt cggacctgcc
     6721 gcgcctgcac gccgagctcg tcgcgctcga tctcggcaag tcgaacgtga acaagctgac
     6781 cgatgtgatc acgtgcccgg gtctcgattt ctgcgacctc gcaaacgcgc gcagcattcc
     6841 gctcagcctc gagatcgccg agcgcttcga tcgcatcgac tatctcaacg acatcggcga
     6901 cgtcagtatc aagatgagcg gctgcatcaa cgcgtgcggt caccatcacg tcggcaacat
     6961 cggcatcctc ggcatcgata agcgcggcga ggagtggtac cagctgatgc tcggcggcag
     7021 cccgggaaac gacgcgtcga tcggtcagat cctcggcgct gcactggcgc aggacgagat
     7081 cgtcgatgcc gtcgagcgcg tgatcgagcg ctatctggaa atccgcgagt cggcggacga
     7141 gcgcttcatc gagaccgtgc ggcgcgtcgg tttggatccg ttcaaggtcg cggtctacgg
     7201 cgcgccacgc actgcgctgg cggcgggagc atgacgatgc agatcattcg caaccgcacg
     7261 atcgtcgacg atgacttcgt ccacgtgccc gacggcgcgg agctccccga cggcaagccg
     7321 atcgtaacgc tcgcgcgcta cctcgccgcg cgggaagagc tgctcgctcg ctaccctgcg
     7381 ctcggcgtcc gcatcgccag tgacaagcta ccgaccgaca ttcccgagct gacgcggctc
     7441 gcgctgatcg cgatcgagtt tccgcgcttc accgacggac gcggctactc ggtggcgcgc
     7501 atgctgcgcg aacgccacaa gttcaccggc gagttgcgcg cggtcggttg ggtgctgcgc
     7561 gacaacctgt tctacatgga gcggtgcggc ttcacggcgt tcgagctgaa gccggggaaa
     7621 ccactcaata gtgccctgga agctttcggc gagctctcgg tgacgtacca agccgccacc
     7681 gacgagaagc ggccgatcta ccgccgccgc taggagccgc cagaagctca tctagctgac
     7741 cgacgacgac gacctgttgc agcgcggtct gtcgaatcgc aaccagtgct tcctcgcgcc
     7801 gcgccggatt cttgctgaat cgcgcgactt cctcccacaa caagatcacc gatagcccgc
     7861 cgcgcagctc gtgagcgagc ttgcgtccag cgagatgtgt cggcttcccc accgacgatg
     7921 tatcgacggc ttcgcgctgc gaggcaagcc ttgcctgaag cgcgtctcgc gccagcgtca
     7981 acggcgtggc acccgcgcac gttctgatcg aaatcgatcc tgatcgcatg gcagcagcgc
     8041 acacttcgat cagcgtacgg ctgtcgatgc ctagcgcgac gtcttcacct cggcgcgcgt
     8101 cgcgttgatc accatgccga cgatgcccgc ggtcacgagc accgcgccca cgacagtgcc
     8161 gccaccgaaa ggtggatcga acgccgggca gttgtcaccg atggtcgccg tcgacatgca
     8221 cttctcgtga gtgagtaggt cggctgcgat cgcaccaccg ccgcccagga tcagcacggt
     8281 attgccggcg tacgcccact tcttcgcgga cgagttgtag ccaaagcaac cgccgagcag
     8341 ggcgacgcat gcgacgaggg ccaccaccct ggacatgtac gaacaatatc acgcgtgcgt
     8401 ggtcggccgc ctcacttgtg ccatcctgcg cgcgcagtgc cccagcggtc tcccctctta
     8461 ccgattttcc tgatcgtgct ggtcgacgtg ctcggcttca cgatcgtgat cccgttgctc
     8521 gggttctacg ccgagaaatt cggtgcctcg cctctcgtcg ccaccacgct cgtctcggtg
     8581 tacgcgctgt gctcactcat ctcgacgccg atcatcggca acctgtctga tcgctacggc
     8641 cgccgccgga tgctgctgat cagccaggcc ggcacgctcg ccgggttcct gatgctcgcc
     8701 aactcgtccg cgctctggat ggtgttcgcc ggccgcatcc tcgacggcac caccgccggt
     8761 aatctgtcgc tcgcgcaggc gtacatctcc gaccacaccg cgcccgagaa tcgcgcgaag
     8821 gcgttcggtg tgatcggtgt cgccttcggc gtcggcttca tgttcgggcc cggtatcggt
     8881 ggcgtgctcg ccgagtacgg catgcacgtg ccgttcatcg cggctgcatc gttgtcactg
     8941 ctgtcgatca tcgcgacgta cacgctgctc ggtaaggaga cgccgccaca gaagacgcaa
     9001 gatggtccgc tacccggcgg caagcgccca ggcgcgttcg acgtcagcac gtacagcgag
     9061 tacttccgcc ggccgatgct cggcaggctc tacctgcagt tcttcatgtt cacgttcgcg
     9121 ttctcgtgct tcacgagcgg cttcgcgctg ttctcggagc gacgcttcac tgccgagcac
     9181 gagatccatc gcgtcgaatc gagctgtgtg ctcgagctcg acacccggat cgaaatgcca
     9241 ggggccgagt tcggcacgat ccgcctcgac ggccgcgcgc tcgcgtacgg ccgcgattgg
     9301 atcgtgttcg gtccggtgat cgtgctgact cacgccgaag actgcgcgcg cctggatagc
     9361 gcgaagctct cggcgcgctg gccatggacc gcgcacgagg tcggcctctt gttcatgttc
     9421 tccggcttcc tcggcatcct gctgcaaggt ggcctgatcg gccggctcgt caaacgctac
     9481 ggcgagccca agctgatcgt cgcgggcttt gccgccgcga gcgtcgcgta cgtcgcgctc
     9541 ggcctcacgt tcacgctctc gctgctgctc gtcgtcgcgg tgctgatgtc attcggcaac
     9601 ggcgtgttgc gtccggtgat cacgagccgg atcacgcagg tcgccggccg tcacgagcaa
     9661 ggcaccgcga tcggcatctc cggatcgctc agctcgttcg cgatgatgct cgcaccaccg
     9721 acgggcggct cgttgatcaa cggccatcac ctgctcggct gggcgctcat cccggcgacg
     9781 gtcaccgcga tcggcctcat catcgcttca ctcccggcaa aaaccgcggg caccaagcaa
     9841 gaccccgagc tatcgtcccc gccatgaaaa ctctcggctg gatggctacc gcgctcgtcg
     9901 caggtggcgt ggcgtttacg atcaagggtg gttgtctgag ctcggagaag gcaccgcccg
     9961 acgcgcggct tgcgggtcat ttcgagaagc tgatgtgcgg catcgcgcgc gacaacatca
    10021 agacgcccga gaagggcgta cgcgagctcg gccgttacat ggacaagcac gccggccaca
    10081 tgttcggcga cttcggcgac atgctcgcga cgatcgaacg catccccgac gacgcgaagc
    10141 acgatcgccg cgccgagaag gcacgcgacc ggctcgcgaa gccagcgatc gcatgcgggc
    10201 gcgactggaa ccggttcggc gaagcgatct ccgacgaccc gaaggcgatg gagttgctcg
    10261 agcggttcaa cgagcgcctc ggccgcacgt tcgagatcat cttcggctct ggccagcact
    10321 tcgacgtgct gcagctgccg cagcagatgc tcggcacgct cgagaaatag cgagaaaatg
    10381 gggtcagacc ggtcagaccc cttttcgacg acggttcgtc cgcccggatc gacggttcgt
    10441 cggatgcccc gttccgcgag ccgcggcgcg gagctacgtg ctgcgcatgc gaaacatcct
    10501 ctgcgcactg gtctgtttac tggccattcc tgtcgtagct gcttgtagcc acgcctcgcc
    10561 gaagccggcg gctccgacac ccgccaccgt cgctacggcc gagcccgagc ggtttgacga
    10621 caaagtccgc gccgacttct ttgacggcat gcgtggcgac gaggccgcga tggcgcgtgc
    10681 gatgaaggtc tgtgacgacg cgctcgcgaa gaacccgaag aacgccgaag cgatggtctg
    10741 gcacggcgcg ggcgtcgttg ctcgcgcgcg cacggcgttc gcggccggca atcgcgacca
    10801 gggaatcgca ctctacacgc agggcatcgg cgagatggac gccgcagtcg cgctcgagcc
    10861 gaacaacgtc ggcgtgcgca tcccgcgcgg cgccgtattc ctcgcgatgg cgccgttcgt
    10921 tcccgagccg gagaagacca agctgctcga gcacggcctc gacgattacg agacgacgta
    10981 cgcgctgcaa cagaaagcgg ggtacttccc gaagctgacg ttgcacgcgc gcgagcagct
    11041 gctctacggg ctcaccgacg gctacgcgaa cctcggcaag gccgacaagg ctgcggccta
    11101 cgtcgagaag atgaagagcg aagcggcggg ctcggagctg ctgccgcggg cggcatcgcg
    11161 tgcgcggggt gaagccgcca acgggccggc accctgcgag cagtgccatc ggaggtaagc
    11221 tggtcccatg gccgagccgt tacgccttcg ggtgacacgc gctgcgctgc tgagcgtcgg
    11281 gctcagcgtc gtgatcggct ttctgttctc gctccacaac ctgacggcga tccggtttgc
    11341 gctgaacaca gcggtctcac tgctctactc gctctcgatc ggcgtgccgg cgacgctgat
    11401 cttcgatcgg ctacagccgc gtatcgggcg gccggtgctg cggcaatggg cgatctacgt
    11461 cggcgtcatg ctcgcgatcg tgctggtggc gtcgttgttc gtcggcgttg tcctcgtgct
    11521 cgtcggcctc atcgagtcgt cgatgctgtg gggcgtattc cggcaaggcc tcgtggtctc
    11581 gctcgtgatc tcgatcccgg tgacgctcgc agtgatcacg gtgtcgcggc tctacgagaa
    11641 gctgggcgac accgaacgcg acaaggcaag agcgctcgca tcggcggccg aagcgcgtct
    11701 tgcgtcgctc gagtcgcgcg tgcgcccgca ctttctgttc aacgcgctca acagcgcgat
    11761 cgcgctgatc ccggacagtc cacgacgcgc cgaggatgtc ctcgagcgac tgagcgcgct
    11821 gctgcgctct tcactcgacg cgcaggcgcg gctcgtgccc ctcggtgacg agctgcgcgt
    11881 cgccaccgat tatct
//
LOCUS       DASUFX010000114         5609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_157431,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000114 DASUFX010000000
VERSION     DASUFX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157431"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFV99_04375"
     CDS             complement(<1..278)
                     /locus_tag="VFV99_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058570.1"
                     /translation="MVGRRLWVVAALALVPQLALADKYEDAFAKTQQLFDAYKASADQ
                     YNAMRAKYDQYYAPIAPQWQVVVTARKVADDECNKNKRTRACRDKTLA"
     gene            complement(366..1454)
                     /locus_tag="VFV99_04380"
     CDS             complement(366..1454)
                     /locus_tag="VFV99_04380"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5058571.1"
                     /translation="MPLQLPFQPPIEPMLAKLSHDIPDGNQWIYEPKWDGFRALVFKS
                     GNDVYIQSRDLKPLGRYFPELEVILREQLPDRCVLDGEIVIATDGRLDFEALLLRIHP
                     AATRVKMLADEKPSSYVAWDLLAFGDEDLRGLPQAKRRERLEEVMAHVRPPVHVTPAT
                     RDKAIAHDWFSRFEGAGLDGVIAKPDDLPYMPGKRLMTKVKHQRTVDCVVAGFRWYKG
                     GKDRLVGSLLLGLYDKAGVLHHVGVCASFKQAERAALTEKLAPYREGALEAHPWKDWA
                     DWQEIETERRLPGATSRWNRGKDLSWVPLRIELTVEVSFDHMQGTRFRHGSHLKRWRP
                     DKPPSECTYEQLEVTPPQEIKDIFGHRG"
     gene            1477..1785
                     /locus_tag="VFV99_04385"
     CDS             1477..1785
                     /locus_tag="VFV99_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058572.1"
                     /translation="MLRALVLVALLSSTAYAERPMCGRNVKYHGARIDLDVKDADIHD
                     VMRLLADVGHVNIVVADSVRGNVTLRLKRVPWDQVACTIAAVHKLTMSVDGNVVMVTK
                     "
     gene            complement(1786..2547)
                     /locus_tag="VFV99_04390"
     CDS             complement(1786..2547)
                     /locus_tag="VFV99_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5058573.1"
                     /translation="MTIPTGRFVWFDYVAKDIAKAQGFFGELFNWKTQEVPMGDGNSY
                     TMITLGDQAIGGYMPTPEGAPAVGHWLSHLQTPDAKATCAKITSLGGKVYKEPAKMGE
                     HGTMAIVADNAGAVLALWQPNKSEGNGDYKDANGAWCWNELAAHDPVKAVAFYSQLGF
                     TEEKMEMPGMGTYHVLNSDGKGRAGVLKSPMPEAPQAWMPYVQVADCDATIAKAKKLG
                     AQIVHEPANVPSVGRIAIFIDPQGGFLGVLQPEKK"
     gene            complement(2638..3084)
                     /gene="msrB"
                     /locus_tag="VFV99_04395"
     CDS             complement(2638..3084)
                     /gene="msrB"
                     /locus_tag="VFV99_04395"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5058574.1"
                     /translation="MPTSRSGYDVTRLSDDRVNALAAGFTPEERHVILHQGTERPFCG
                     LLLDNKMAGTYVCRLCGLPLFSSNSKFESGTGWPSFFKPYDPDHVQNLEDASHGMVRV
                     EIRCARCDAHLGHVFDDGPPPTHKRFCLNSVSMEFVPDGQPIQLRS"
     gene            3281..>5609
                     /locus_tag="VFV99_04400"
     CDS             3281..>5609
                     /locus_tag="VFV99_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5058575.1"
                     /translation="MVCDNHGGCALPDQLSTCTGKPDGTLCSYKDRANATINGACASS
                     LCVPLNCGNMIVSPDEQCDDGNNNSGDGCSADCQSLETCGNGYTDAAKGEQCDDGNAV
                     DGDGCQTNCRLPVCGDGIKDTSLDEECDAGPANANLPDAVCRPNCQLPRCGDGVTDPS
                     NSEICDDGNNASADGCSGDCKSNETCGNNIVDALAGEVCDDGNAVGGDGCSANCRSVE
                     VCGNGVTDTAFGEVCDDGNTVSGDGCSSDCRSLEVCGNGIIDTINEQCDLGAMNSNAA
                     DSACRTNCQLPRCGDTIRDPLHGEQCDDGPMNANAADACRTNCQLPRCGDMIQDTMEV
                     CDDGNTTSGDNCSSDCRSLETCGNGIVDTVKGEQCDTGAANSNNPNATCRPDCKTQRC
                     GDTIVDTAFGEGCDLGTMNSNAANAMCRVNCQPQRCGDGVKDNLMGEVCDDGNVVSGD
                     NCSADCKSNETCGNGIVDAAKGEQCDAGAMNANTANAPCRTNCQLPRCGDSAVDTNLG
                     EACDLGASNSNAANAFCRTNCQPQRCGDGIADTTKGEVCDDGNATAGDGCSPDCKSNE
                     TCGNGVVDASKGEQCDAGAANSNNPNAACRTNCQLPKCGDNVVDSSLGETCDAGAMNS
                     NAANATCRTNCQPQRCGDSILDNTKGEVCDDGNVVSGDMCSADCKSNETCGNGVIDVV
                     KMEQCDNGALNSNSPNATCRPTCVSQKCGDSIVDNAFGEGCDLGAANSNSPNAMCRVN
                     CTPRRCGDGVIDSASGEICDDNNTTSGDGCSADCKS"
BASE COUNT         1055 a         1772 c         1830 g          952 t
ORIGIN      
        1 tatgcgagcg tcttgtcgcg acacgcgcgc gtgcgcttgt tcttgttgca ctcgtcgtcg
       61 gcaaccttgc gtgcagtgac gacgacctgc cattgcggcg cgatcggcgc gtagtactga
      121 tcgtacttgg cgcgcatcgc gttgtactga tcggcggaag ccttgtacgc atcgaacagc
      181 tgctgcgtct tggcgaacgc gtcctcgtat ttgtctgcga gcgcgagctg cggaacgagc
      241 gcgagcgcgg ctaccaccca caagcgtcta ccgaccatag gtacatggta gcgccgcaaa
      301 cgtcgcgtct cggtagagcc tcggtcaacg gtcggttagc cgccggtcgg ttagccgccg
      361 gtcggttagc cgcgatgacc aaagatgtct ttgatctcct gcggaggcgt gacctcgagc
      421 tgctcgtagg tgcactccga cggcggctta tccgggcgcc agcgcttcag atgcgagccg
      481 tggcgaaacc gcgtgccctg catgtgatcg aacgaaacct cgacggtgag ctcgatgcgg
      541 agcggcaccc acgacagatc cttgccacgg ttccaccggc tcgtcgcacc aggcagccga
      601 cgctcggtct cgatctcctg ccagtcggcc cagtccttcc acggatgcgc ctcgagcgcg
      661 ccttcgcggt acggcgcgag cttctcggtg agcgcagcgc gttcggcttg cttgaacgac
      721 gcgcacacgc cgacgtgatg gagcacgccg gccttgtcgt agagcccgag caggagcgag
      781 ccgacgaggc gatccttgcc gcccttgtac caacgaaagc cggcgacgac gcagtcgacg
      841 gtgcgctggt gcttgacctt cgtcatcagg cgcttgcctg gcatgtacgg caggtcgtcg
      901 ggtttcgcga tgacgccgtc gaggccagcg ccttcgaagc gcgagaacca atcgtgcgcg
      961 atcgccttgt cgcgcgtcgc cggcgtgaca tggaccggcg gccgcacgtg cgccatcacc
     1021 tcctcgagcc gctcgcggcg cttggcctgc ggtaagccgc gcagatcctc gtcgccgaat
     1081 gcaagcaggt cccacgcgac gtacgacgat ggtttctcgt cggcgagcat cttcacgcgc
     1141 gtcgcggctg gatgaatgcg caagagcagc gcttcgaagt cgagccggcc atcggttgcg
     1201 atcacgatct cgccgtcgag cacgcagcga tcgggcagct gctcgcgcaa gatgacctcg
     1261 agctcgggga agtagcggcc gagcggcttc agatcgcggc tctgtatgta cacgtcgttg
     1321 ccagacttga agaccagcgc gcgaaagccg tcccacttcg gctcgtaaat ccactgattg
     1381 ccgtcgggaa tgtcatgcga cagcttcgcg agcatcggct cgatcggcgg ctgaaaaggc
     1441 aactgcagcg gcaccgcgag aagctacact gaactcgtgc tgcgcgctct cgtcctcgtc
     1501 gcgttgttgt cgtcgacagc gtacgcagag cgtccgatgt gtgggcgcaa tgtgaagtat
     1561 cacggcgcgc ggatcgatct cgacgtcaag gacgccgaca tccacgacgt gatgcggctg
     1621 ctcgccgacg tcggccacgt caacatcgtc gtcgcggatt ccgtgcgcgg caacgtcacg
     1681 cttcgcttga agcgcgtgcc gtgggatcaa gtcgcgtgca cgatcgctgc cgtccacaag
     1741 ctcacgatga gcgtggacgg caacgtcgtg atggtgacga agtaactact tcttctcggg
     1801 ctggagcacg ccgaggaaac cgccctgcgg atcgatgaag atcgcgatgc gtccgacgct
     1861 cggcacattc gccggctcgt ggacgatttg cgcgccgagc ttcttcgcct tcgcgatcgt
     1921 cgcgtcgcaa tcagccactt gcacgtacgg catccacgct tgtggcgcct cgggcatcgg
     1981 cgacttcagc acaccagcgc gacctttgcc gtcgctgttc agcacgtggt acgtgcccat
     2041 gcccggcatc tccatcttct cttcggtgaa gccgagctgc gagtaaaacg cgactgcctt
     2101 caccggatcg tgagcggcga gctcgttcca gcaccacgcg ccgttcgcat ccttgtagtc
     2161 gccgttgcct tcggacttgt tcggctgcca cagcgcgagc acagcgccgg cgttgtcggc
     2221 gacgatcgcc atcgtgccgt gctcgcccat cttcgccggc tccttgtaga ctttgccgcc
     2281 gagcgacgtg atcttcgcgc acgtcgcctt cgcgtcgggc gtttgcaggt gcgagagcca
     2341 atgaccgacg gccggcgcac cctcaggcgt cggcatgtag ccgccgatcg cttggtcacc
     2401 gagcgtgatc atcgtgtacg agttgccatc gcccatcggg acttcttgcg tcttccagtt
     2461 gaagagctcg ccgaagaagc cctgggcctt cgcgatgtcc ttcgccacgt agtcgaacca
     2521 cacaaacctg ccagtcggga tcgtcatgtt cgtatctcct tcgttgtgcc cgagggtcga
     2581 atggcgcggt gacggatcga catcgccttc gaaaaactcg ccgaatgcgt taaccggtca
     2641 ggatcgcaac tggatcggct gcccgtcggg cacgaactcc atcgatacgg agttcaagca
     2701 gaagcgctta tgggtcggcg gcggtccgtc gtcgaagacg tgtcccagat gtgcgtcgca
     2761 gcgcgcacac cggatctcga cacgcaccat tccatggctc gcgtcctcca aattttgcac
     2821 atggtcgggg tcgtacggct tgaagaagct gggccaaccg gtgccactct cgaatttcga
     2881 gttgctcgag aacagcggca aaccgcacag cctgcacaca taagtgccgg ccattttgtt
     2941 gtctaacagc aggccacaaa aggggcgctc tgtcccttgg tgaaggatga cgtggcgctc
     3001 ttctggcgtg aagccagccg cgagcgcgtt gacgcgatcg tcgctgagac gggttacatc
     3061 gtagccagag cgggatgtcg gcatagcggg atccttacga tgcagggatg caaaagttac
     3121 gtgcttccga tattgcgcga aacgggccca acccaggtac catgacaaag tggtcgtttc
     3181 aggcctcctg agggggatgg ctctcgtaac gctgtgcgcg cttaccgcgt gtttcagcgc
     3241 cgagattaca cattgtccgt cggttgattg cccgaaggaa atggtctgcg acaaccacgg
     3301 cggttgcgca ctacccgatc agctatcgac gtgcactgga aagcctgacg gcacgctctg
     3361 cagctacaag gatcgcgcga acgcgacgat caatggtgcg tgtgcgagca gcctgtgcgt
     3421 gccgctcaac tgcggaaaca tgatcgtcag cccggacgag cagtgcgacg acggtaacaa
     3481 caacagtggc gacggttgct cggcagattg tcagtcgctc gagacgtgcg gcaacggcta
     3541 cacggatgcg gccaaaggcg agcaatgcga tgacggcaac gctgtcgacg gcgatggctg
     3601 tcagacgaac tgccggctgc cggtctgcgg tgacggtatc aaggacacca gcctcgacga
     3661 ggaatgcgat gcgggtcccg cgaacgcgaa cctgccggac gcggtgtgcc ggccaaactg
     3721 ccagctaccg cgctgtggtg acggcgtcac ggatccgagc aatagcgaga tctgcgacga
     3781 cggtaacaac gcgagcgcgg acggctgctc gggtgactgc aagtcgaacg agacgtgcgg
     3841 caacaatatt gtcgatgcgc tcgcaggtga agtctgcgat gacggcaacg cggtcggcgg
     3901 cgatggctgc tcggcgaact gccgctctgt cgaggtgtgc ggcaacggcg tgaccgacac
     3961 ggcgtttggc gaagtctgcg acgacggcaa cacggtgagc ggcgatggct gctcgtcgga
     4021 ctgccgctcg ctcgaggtct gcggcaacgg catcatcgat acgatcaacg agcagtgcga
     4081 cctcggcgcg atgaactcga atgctgccga ctcggcgtgc cgcacgaact gccagttgcc
     4141 gcgctgcggc gacaccatcc gcgacccgct gcatggcgag caatgcgacg acggcccgat
     4201 gaacgcgaac gctgcggacg cgtgccgcac gaactgccag ctgccgcgtt gcggcgacat
     4261 gatccaggac acgatggagg tctgcgacga cggcaacacg acgtcgggcg acaactgctc
     4321 cagcgactgt cgctcgctcg agacctgcgg caacggcatc gtcgacacgg tcaagggcga
     4381 gcagtgcgac accggcgcgg ccaacagcaa caacccgaac gcgacgtgcc ggcccgactg
     4441 caagacgcag cgctgcggcg acacgatcgt cgacacggcg ttcggcgagg gctgcgatct
     4501 cggcacgatg aactcgaacg cggcgaacgc gatgtgccgc gtcaactgcc agccacagcg
     4561 ctgcggcgac ggcgttaaag acaacctgat gggcgaagtc tgcgacgacg gcaacgtcgt
     4621 gtcgggtgac aactgctcgg cggactgcaa gtcgaacgag acctgcggca acggcatcgt
     4681 cgacgccgcg aagggcgagc agtgcgatgc cggcgcgatg aacgcgaaca ccgcgaacgc
     4741 accgtgccgt acgaactgcc agctgccgcg ctgcggcgac agcgccgtcg acaccaacct
     4801 cggcgaggcg tgcgacctgg gcgcttcgaa ctcgaacgcg gcgaacgcgt tctgccgcac
     4861 gaactgccag ccacaacgct gtggtgacgg catcgcggac accacgaagg gcgaagtctg
     4921 cgacgacggc aacgcgaccg ccggcgatgg ctgctcgccg gactgcaagt cgaacgagac
     4981 ctgtggcaac ggcgtcgtcg acgcgtcgaa gggcgagcag tgcgacgcgg gcgcggcgaa
     5041 ctcgaacaac ccgaacgcgg cgtgccggac gaactgccag ctgccgaagt gcggtgacaa
     5101 cgtcgtcgac agctcgctcg gcgagacctg cgacgccggt gcgatgaact ccaacgctgc
     5161 gaacgcgacg tgccggacca actgtcagcc gcagcgctgt ggcgacagca tcctcgacaa
     5221 cacgaagggt gaggtctgcg acgacggcaa tgtcgtgtcg ggcgacatgt gctcggcgga
     5281 ctgcaagtcg aacgagacct gcggcaacgg cgtcatcgac gtcgtgaaga tggagcagtg
     5341 cgacaacggc gcgctcaact cgaacagccc gaacgcgacg tgccgcccga cctgtgtgtc
     5401 gcagaagtgt ggcgacagca tcgtcgacaa cgcgttcggc gagggctgtg acctcggcgc
     5461 ggcgaactcg aatagcccga acgcgatgtg ccgggtcaac tgcacgccgc ggcgctgtgg
     5521 cgacggtgtc atcgatagcg cttcgggcga gatctgcgac gacaacaaca cgacgtcggg
     5581 tgatggctgc tcggcggact gcaagtcgc
//
LOCUS       DASUFX010000115         6906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1686558, whole genome shotgun sequence.
ACCESSION   DASUFX010000115 DASUFX010000000
VERSION     DASUFX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686558"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFV99_04405"
     CDS             <1..529
                     /locus_tag="VFV99_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058576.1"
                     /translation="ESVSEVLDHVADRLTHGHANADAYVLAVVELATNPDMATVLHGL
                     VAAGSALPDVGAATNALKTIDPAALSRLQQLLFGDPLETAAKDLMKARRLGVVVLGHT
                     HSVGGFVRHVDGGYYANTGSWISVASVAELRARGIRWEQLSILDRATFPSKMTAIVID
                     YNGTTPRAPVVENQR"
     gene            complement(534..851)
                     /locus_tag="VFV99_04410"
     CDS             complement(534..851)
                     /locus_tag="VFV99_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX5058577.1"
                     /translation="MMLRAAAICVALLACHKHEPRTYQVAIKGMQFVPAELSVEIGDT
                     VVWTNEDILPHTVTSGIPAPATFDSREIASKKQWSLTVSAAGEYTYNCTYHPPMTAKL
                     IVR"
     gene            complement(848..1453)
                     /locus_tag="VFV99_04415"
     CDS             complement(848..1453)
                     /locus_tag="VFV99_04415"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX5058578.1"
                     /translation="MSFTPACKKKESTSAPPPSAPAVTEQAPAPAPAPEPAPAGPALS
                     DAKIAAIVVAANQVDVDAGQLALKKSKNAEVKKFAERMVTDHTAVNKSAVDLVTKLKV
                     TPEESDVSKSLTTGGTAKRAELEKLDGDAFDKAYVDNEVAYHEAVIGVLKSQLIPAAS
                     NQELKDALVGVQPAFDAHLEHAKKIQEALASGGSADHAHHK"
     gene            complement(1563..1751)
                     /locus_tag="VFV99_04420"
     CDS             complement(1563..1751)
                     /locus_tag="VFV99_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058579.1"
                     /translation="MAAVLCLVLLPLMAAAGFMLGVVNGQFSAASLLGGVTFVVLGFG
                     MFFGIFRMSRDWENEQEH"
     gene            complement(1860..3455)
                     /gene="norR"
                     /locus_tag="VFV99_04425"
     CDS             complement(1860..3455)
                     /gene="norR"
                     /locus_tag="VFV99_04425"
                     /inference="COORDINATES: protein motif:HMM:NF003451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HEX5058580.1"
                     /translation="MCTVRGRDNRPRCTDAVRSYRSDNNMLEPLLQFASDLAADLTPS
                     ERYQRFLALARRMIPCDSVVLLRMDEGAFVPIAVDGLRAEAIARRYHPDDHPRLARIR
                     EARAPVRFDDTGMPDPFDGLFAHAGDALSRAHSCMGCAVLVDSDVIGAVVFDALTPGC
                     FDRIDLENVSMLATLAGAAIRTSALSSALAQLGAPETGKTRQLVREALDRMGGNLLGT
                     SDQLARVRREIDLLARSELTALITGETGVGKELVAQAIHTKSSRADKPMVVVNCAALP
                     DSLAESELFGHLRGAFTGAVDHRAGKFEVADGGTLFLDEVGELPLAVQSKLLRALQYG
                     EVQRIGSDRLLRVDVRVIAATNRDLGEEVRAGHFRADLLYRLGVFPLHIPPLREHPED
                     IGVLSGHFLDLARERLGVGPIRLAPAARKVLREHGWPGNVRELEHTLMRGSLRASERY
                     RGGTVLIDVADLGLADPAPVARTSSGKAPRLRDAVDGFTKDLISTTVRECDGNWAEAA
                     RRLGLQRGNLHRLATRLRLHGRA"
     gene            3477..3848
                     /locus_tag="VFV99_04430"
     CDS             3477..3848
                     /locus_tag="VFV99_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058581.1"
                     /translation="MQQDHEQLDLELGRLAQALRGGDHMLACLQLAEFALKLDIYIRR
                     EERALSFAHRCVGAPPRAVTIIQREHASLRELVASIASALDRADDRRGVEMIGKLRSV
                     LLFHMVKEQLVLFERTAAETW"
     gene            complement(3856..4827)
                     /locus_tag="VFV99_04435"
     CDS             complement(3856..4827)
                     /locus_tag="VFV99_04435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5058582.1"
                     /translation="MDRFGPPEVVHTEVLPVPRVGKHDVLVRVATAGVGAWDPDLVDG
                     SFQDVKPRFPRVVGSDGAGTVVAVGDDVENFAVGDRVYGWGLGNAKGGFFAEYISINE
                     REIALVPDSVSFEEAGALAVSGITALAGLDELNLSEGQSLIIFGASGGVGHVAVQLAK
                     ALGLRVFAVASKDDGVELVKRLGADAVAEGHSKTLLRELREFAPDGFDGALVFAGGHG
                     WKNELELVVKGGKVAWPNGVQPAPEVPKGVNRASFNGEESPEAFAQLNQLAARRPLHV
                     ELSKVYPLDATAQALRDVHEHHIGKLAIQIEAVGGHRTKRGPSTHRH"
     gene            4960..6174
                     /locus_tag="VFV99_04440"
     CDS             4960..6174
                     /locus_tag="VFV99_04440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5058583.1"
                     /translation="MLAPRMCCLDLDTFFVSVERLLDPSLRGKPVVVGGRRGGRGVVT
                     SASYEVRAFGVRSGMSIREASERAPHDTVFVPGHHDEYTSYSKRARDIVERFSPVVVA
                     ASIDEHYLDFRGCERLYRRHGDADDDATILRVVREMTKAIESELGLPASAGIATSKPM
                     AKVASGLAKPNGVLLVAAGTEAATLAPLPTRKLPGIGPVSETRLRAIGVETLGQLAAA
                     PDTMLRRIFGAYTGSILLAARGGGTDDLGRERPAFREHDPHGVVAGTISNERTFVESN
                     AETAHVVLSGLCERVCSRARRRGVVAGQVTLKLRYTDFHTITRARSITPTSVDVEMHR
                     IVLELYREARTRPLPIRLLGVAVAKLRLDTVQLPLFDAGERRGIVVDRVRDKFGYDAV
                     HLATTLSRGRRR"
     gene            complement(6171..6827)
                     /locus_tag="VFV99_04445"
     CDS             complement(6171..6827)
                     /locus_tag="VFV99_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5058584.1"
                     /translation="MPTKLLNDDGTASMATMIMTSHHAFHRDVACFAKALAETTRVVD
                     VLREEWTRFRGALHGHHTVEDTGLFPDMRAKHPELAAAIDKLDEQHRAIDPLLERGDQ
                     VFADLANQRAAAQDVIASLAALLAEHLDAEEAAVIPHLRAAKQFPLPPSDDALAMYAE
                     GFAWSTAGLASTVLEQVFAMLPPALVEKIPAARSAFDDRSRRVWGYTHTGASVTSSPA
                     "
BASE COUNT         1212 a         2300 c         2215 g         1179 t
ORIGIN      
        1 cgagtcggtg agcgaggtgc tcgatcacgt cgccgatcgc ctgacgcacg ggcacgcgaa
       61 tgccgatgcg tacgtcctcg ctgtcgtcga gctggcgacg aacccggaca tggccactgt
      121 actgcacggt ctggtagccg cgggtagtgc cctgcccgac gtcggtgctg cgaccaacgc
      181 gctcaagacg atcgacccgg cggcgctctc gcggctccag caactgctgt tcggtgaccc
      241 actcgagacg gcagcgaagg atctgatgaa agcgcgccgc ctcggcgtcg tggtgctcgg
      301 ccatacgcac tcggtcggcg gcttcgtgcg acacgtcgat ggtggctatt acgcgaacac
      361 cggctcgtgg atctcggtgg catcggtcgc ggagctccgc gcgcgcggca tccgctggga
      421 gcagctatcg attctcgatc gcgcgacgtt tccgtcgaag atgaccgcga tcgtcatcga
      481 ctacaacggc acgacaccac gtgcgccggt cgtcgaaaac cagcggtgat cggtcagcgc
      541 acgatgagct tcgccgtcat cggcgggtga tacgtgcagt tgtacgtgta ctcaccggcc
      601 gcagatacgg tcaggctcca ctgcttcttg ctcgcgatct ctcgcgagtc gaatgttgca
      661 ggggccggga taccagacgt cacggtgtgg ggaaggatgt cttcgttcgt ccacaccacg
      721 gtatcgccga tctccacgga cagctcggcg ggcacgaact gcatgccctt gatcgcgacc
      781 tggtacgtac gcggttcgtg cttgtgacag gcgagcagcg cgacgcaaat tgcggccgcg
      841 cgcagcatca tttgtgatgc gcgtggtcgg cggatccacc gctggctagc gcctcctgga
      901 tcttcttcgc gtgctcgaga tgcgcgtcga acgcgggctg cacgccgacc agcgcatcct
      961 tgagctcttg attgctcgcg gcaggaatca gctggctctt gagcacgccg atcacggcct
     1021 cgtgataggc gacttcattg tcgacgtacg ccttatcgaa cgcgtcgccg tcgagcttct
     1081 cgagctcggc acgtttcgcg gtgccgcccg tcgtgagcga cttgctgacg tcggactcct
     1141 ccggcgtcac cttcagcttc gtcacgaggt cgacggccga cttgttgacg gccgtgtggt
     1201 cggtaaccat gcgctcggcg aacttcttca cttcggcgtt cttcgacttc ttgagcgcga
     1261 gttgcccggc gtcgacgtcg acttgattcg ccgcgacgac gatcgccgcg atcttcgcgt
     1321 cggacagagc cggtccagca ggcgccggct cgggcgcggg agctggggcc ggcgcttgct
     1381 ccgttacagc gggcgcagac ggcggcggcg cggacgtgga ttccttcttc ttgcacgccg
     1441 gcgtaaacga catgcaaaag agaaagagcg cagatgcaac aaaccaacgt gctctcatcg
     1501 tgttctccag atgcgtggtg acactgcaat ggagagtgac cggcgccgaa acgtgacaga
     1561 cgtcagtgct cttgttcgtt ttcccagtcg cgagacattc tgaagatgcc gaagaacatg
     1621 ccgaacccga gcacgacgaa cgtgacgccg ccgagcaggc tcgcggcaga gaactgaccg
     1681 ttgacgacac cgagcatgaa gccagcggcc gccatcagtg ggagcagaac gagacacaac
     1741 acagctgcca tgcagcagtg cattgcgatg ttcgtgccgg ctgcggctag acggacaggc
     1801 tcctgaatcg cgagcgcgtg ttgtctttcc gagaacgagc ggctcgaaaa gagaacggct
     1861 caggctcgac cgtgcaaccg cagccgggtc gcgagccggt gcaaattccc gcgctgcaac
     1921 ccgagccgac gcgcggcttc tgcccagttg ccgtcgcatt cgcggacggt cgtgctgatc
     1981 agatccttcg tgaaaccgtc gacggcgtcg cgaagacgag gcgccttgcc ggagctggtg
     2041 cgcgccaccg gcgcaggatc tgcgagaccg aggtccgcga cgtcgatgag cacggtgccg
     2101 ccgcgataac gttcggacgc tcgcagcgaa ccgcgcatca gcgtgtgctc gagctcgcga
     2161 acgttgcccg gccaaccatg ctcgcgcaga accttgcgcg ccgcaggtgc cagccggatc
     2221 ggaccgacgc caagtcgctc gcgcgcgaga tcgagaaagt gcccgctcag cacgccaatg
     2281 tcttcggggt gctcgcgcag cggcggaatg tgcagcggga acacgcctag ccggtacagc
     2341 aagtccgcgc gaaagtgccc cgccctgacc tcttcgccta gatcgcggtt cgtcgccgcg
     2401 atgacgcgca cgtcgacgcg cagtagccga tcagacccga tccgctggac ttcaccatac
     2461 tgcagggcgc gcaacagctt cgactggacg gcgagcggta gttctccgac ctcgtcgagg
     2521 aagagcgtgc cgccatcggc cacctcgaac ttgccggcgc gatgatcgac ggctcccgtg
     2581 aacgcaccac gcaaatggcc gaacagctcg ctctcggcga gagagtccgg cagtgcagcg
     2641 cagttgacca cgaccatcgg cttgtcggcg cgcgacgatt tcgtatgaat cgcctgcgcg
     2701 acgagctcct tgccgacacc ggtctcaccc gtgatcagcg ccgtgagctc cgagcgagcg
     2761 agcagatcga tctcgcgtcg aacgcgggcg agctgatcgc tagtgccgag gaggttccct
     2821 cccatgcgat cgagcgcctc gcgaacgagc tgacgtgttt ttccggtttc cggtgctccg
     2881 agctgggcca acgcggatga gagcgcgctc gtcctgatcg cagcccccgc caaggttgcg
     2941 agcatcgaga cgttctcgag gtcgatgcga tcgaagcaac cgggcgtcag cgcgtcgaac
     3001 acgaccgctc cgatcacatc ggaatcgacg agcaccgcgc agcccatgca cgaatgcgca
     3061 cgcgacagcg cgtcgccggc gtgggcgaac aggccatcga atggatcggg catcccggtg
     3121 tcatcgaagc gaaccggcgc ccgcgcctct ctgatgcgcg cgagtcgagg gtggtcatca
     3181 ggatgataac gacgagcgat cgcctccgcg cgaagtccgt cgaccgcgat ggggacgaat
     3241 gcaccttcgt ccatccgcaa gagcacgacg gaatcacacg ggatcattcg gcgtgcgagc
     3301 gcgaggaaac gctgatagcg ctcggacggg gtcaagtcgg cggcgagatc cgatgcgaac
     3361 tgcagcagcg gttccagcat gttgttatcg gatcgatagc tccggacggc gtcggtgcag
     3421 cgtggccgat tgtcgcgccc gcgaacggtg cacattgctt gcaacacaaa cgatctatgc
     3481 agcaggacca cgagcaactc gacctggaac tgggacgtct cgcgcaggct ctacggggcg
     3541 gcgatcacat gctcgcgtgc ctccagctgg ccgaattcgc gttgaagctc gacatctaca
     3601 tccggcgtga agagcgcgct ctgtcgtttg ctcatcgctg cgtcggggcg ccgccgcgcg
     3661 cggtcacgat cattcaacgc gagcacgcca gcttgcgcga gctcgtcgca tcgatcgcga
     3721 gcgccctcga ccgcgccgac gatcgccgcg gcgtcgagat gataggcaag ctgcgaagcg
     3781 tgctgttgtt ccacatggtc aaggagcagc tcgttctctt cgagcgcacc gcagccgaaa
     3841 cttggtaagc gtcacctagt gccgatgcgt tgaagggccg cgcttggtgc ggtgtccgcc
     3901 cacggcttcg atctggatcg cgagcttgcc gatgtggtgc tcgtgcacgt cgcgcagcgc
     3961 ctgcgcggtt gcatcgagcg gatagacctt cgatagctcg acgtgcaacg gacgacgtgc
     4021 agcgagctgg ttcagctgag cgaacgcttc tggcgattcc tcgccattga agctcgcgcg
     4081 attcacgccc ttcggaacct caggtgccgg ttggacgcca tttggccacg cgaccttgcc
     4141 ccctttcacg acgagctcga gctcattctt ccagccatgg ccgcccgcaa acacgagcgc
     4201 gccatcgaat ccatcgggtg cgaactcgcg aagctcgcgc agcaacgtct tgctgtgtcc
     4261 ctctgcgacg gcatcggcgc cgagtcgttt caccagctcc acgccatcat ccttcgatgc
     4321 aaccgcaaac acgcgcagcc cgagcgcctt cgcgagctgg accgccacgt gaccgacacc
     4381 gccgctcgcg ccgaagatga tcaggctctg tccttcggag agatttagct cgtcgagccc
     4441 ggcgagcgcc gtgatccccg agaccgctag cgcacccgct tcttcgaacg agacggagtc
     4501 cgggacgagc gcgatctcac gttcgttgat cgaaatgtac tcggcaaaga agccgccctt
     4561 cgcattgccg agcccccagc catacacgcg atcgccgacg gcaaagttct cgacgtcgtc
     4621 gccaaccgcg acgaccgtac ctgctccatc ggagccaacc acacgcggaa accgcggctt
     4681 gacatcctgg aacgaaccgt cgacgagatc cggatcccac gcaccgacgc ccgccgtcgc
     4741 cactcgcacg agaacatcgt gcttacccac gcgcggtacc ggcaacacct cggtatgaac
     4801 gacctcgggc ggtccgaacc tgtccatcgc cgccgctctc atcgttgtcg ggatctctcg
     4861 cgctgtcgcc atgcgaacct ccgcatccaa gcgctgcaat ctcaagaccc tgagcacact
     4921 gccgttgcct gaacgcatgt tcagatcgag aatcgaacga tgctggcgcc gcggatgtgc
     4981 tgcctcgatc tcgacacgtt cttcgtgtcc gtcgagcggc tcctcgatcc gagcttgcgc
     5041 ggaaagccgg ttgtcgtcgg tgggcgtcgc ggcggacgcg gcgtcgtgac gtcggcgagc
     5101 tacgaggtgc gtgcgttcgg cgtgcgatcc ggcatgtcga tccgcgaggc gtcggagcgc
     5161 gcgccgcacg acaccgtgtt cgtgcccgga caccatgacg agtacacgag ctactcgaag
     5221 cgagcgcgag acatcgtcga gcgattcagt cccgtcgtcg tcgccgccag catcgacgag
     5281 cactacctcg actttcgcgg ctgcgagcga ctgtaccgcc ggcatggcga cgccgacgac
     5341 gacgcgacga tcctgcgcgt cgtgcgcgag atgacgaagg cgatcgagag cgagctcggc
     5401 ctcccggcga gcgccggcat cgccacgagc aagccgatgg caaaagtcgc gagcgggctc
     5461 gcgaagccaa acggcgtcct gctcgtggcg gcaggcaccg aggctgcgac gctggcgccg
     5521 ctgccaacac gcaagctgcc cggcatcggc cccgtatccg agacgcgatt gcgtgcgatc
     5581 ggcgtcgaga cactcggcca gctcgccgca gcgcccgaca cgatgcttcg ccgcatcttc
     5641 ggcgcgtaca ccggctcgat cctgcttgcc gcgcgcggcg gcggaaccga cgacctcggc
     5701 cgcgagcgac ctgcattccg cgagcacgat ccgcacggtg tcgtcgccgg cacgatctcg
     5761 aacgagcgca cgttcgtcga gtcgaacgcg gagaccgcgc atgtcgtgct gtcgggacta
     5821 tgcgagcgcg tgtgctcgcg tgcgcgccgg cgtggtgtcg tcgccggcca ggtcacgctc
     5881 aagctccgtt acaccgactt ccatacgatc acgcgcgcgc gctcgatcac acccacgagc
     5941 gtcgacgtcg agatgcatcg catcgtgctc gagctgtatc gcgaagcgcg cacacgaccg
     6001 ttgccgattc gcctgctcgg cgtcgcggtc gcgaagcttc gtctcgacac ggtgcagctg
     6061 ccgttgttcg atgcaggcga acgccgcggc atcgtcgtcg atcgcgtacg cgacaagttt
     6121 ggctacgacg ccgttcatct cgcgaccaca ctctcgcgcg gtcgtcgccg ttaggcgggc
     6181 gaagacgtga ccgacgcgcc ggtatgcgtg tacccccaga cgcggcggct gcggtcgtcg
     6241 aaagcgctgc gagcggcggg aatcttctcg acgagtgcgg gcggcagcat cgcgaacacc
     6301 tgctcgagca ccgtgctcgc gaggccggcc gtgctccacg cgaagccctc ggcgtacatc
     6361 gcgagcgcgt catcgctcgg cggcaacggg aactgcttcg cggcgcgcag atgcggaatg
     6421 accgcggcct cctcggcgtc gaggtgctcg gcgagcaatg ccgcgagcga cgcgatcaca
     6481 tcttgcgccg cagcgcgctg gttcgcgagg tcggcaaaca cctggtcgcc gcgctcgagt
     6541 agcggatcga tcgcgcgatg ctgctcgtcg agcttgtcga tcgctgcagc cagctcgggg
     6601 tgcttggcgc gcatgtcggg aaacagcccg gtgtcttcga ccgtgtgatg accgtgcagc
     6661 gcgccgcgga accgcgtcca ctcctcccga agcacatcga cgacgcgcgt cgtttccgcc
     6721 agcgccttcg cgaagcacgc cacgtcacgg tggaacgcgt ggtgcgaggt catgatcatc
     6781 gtggccatcg aggccgtgcc gtcgtcgttg aggagcttgg tgggcatcgg attcttacct
     6841 ttcgcctaca cgacgaatga cgacggccgc gttcgacatg ccgcgtgaac ttttctgcta
     6901 agtccg
//
LOCUS       DASUFX010000116         8928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1965018, whole genome shotgun sequence.
ACCESSION   DASUFX010000116 DASUFX010000000
VERSION     DASUFX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965018"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFV99_04450"
     CDS             <1..438
                     /locus_tag="VFV99_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058585.1"
                     /translation="EANDVIVLAHTRSSAPVAYEVATRLGLPLELFDHGSHVADKTVL
                     LIDDGDDARGMVRSISELRERAAVMVVAATAVASPAVYTMLRTAADHVSCILSPQHIY
                     SVQAWFADFEPPNDEEIQQLLAAAAQNLLLLRRSNFLTSGVDT"
     gene            514..1104
                     /locus_tag="VFV99_04455"
     CDS             514..1104
                     /locus_tag="VFV99_04455"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5058586.1"
                     /translation="MNSRSSSESPKLRRILERAARLIFEKGYEGTSMQDIADACGLTK
                     AGLYHHVATKEVLLIEIMEYGMDLFEEVVLARAEPIRDPLERLKKTMACNIELVTTDS
                     SKEVTIILHEHQTLTGEAQKKINGRKKRYVRFLEDAFKQAMAAGQIRNVDPTLASFSF
                     LGTVLWTYKWYRPDGRLTPQQLSDGTIDLFFNGLVA"
     gene            1115..1918
                     /locus_tag="VFV99_04460"
     CDS             1115..1918
                     /locus_tag="VFV99_04460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5058587.1"
                     /translation="MTRWVRLDEAAAAVPDGAWIAPGGFMLGRAPMALIFALIRAEKR
                     LRVISLPNPLPAEMLVATNLAARVELLFAALTLANRVRPMPALKRAIERGEVPWAEHD
                     GYRIVQRLRAAAMGLPFLPAPDADVSELSTLEPPSYTLDPFTGARVPVERAFHPDVAL
                     VHAHAADDAGNLYIEDPTTDLLVVGAAHRVIATAEVRVRQLTRVTVPAFQVDVVAEAT
                     GGAWPTGCVGLYPHDEKALLAYLAASEAGEGADWIQSVLQGRIPLRGAA"
     gene            1915..2643
                     /locus_tag="VFV99_04465"
     CDS             1915..2643
                     /locus_tag="VFV99_04465"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5058588.1"
                     /translation="MITPAERLVAQMAGEIHDGAVVATGVASPLAVLAIAVARATHAP
                     RLTYIACVGSLNPNVTRLFGSSEDLAYLDGREAEISIPDLFDHARRGRVDTVFFGAVE
                     VDGEGRTNLSAAGPLASPKIKFPGVVGACSLRRWCKNPVLVVPRQNARCLVPRVNVTS
                     TEDVRRTRLLTDLAVFSIGEGGAILEAIAPHASLADVRVRTGFAYGEAAPIAHFTDPD
                     ADVLAAIRRIDPDNLRDELVGQLN"
     gene            2655..3689
                     /locus_tag="VFV99_04470"
     CDS             2655..3689
                     /locus_tag="VFV99_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5058589.1"
                     /translation="MEAVVLSQFGSAHYLAMQSVPDPHPGPGEVLLRVRACGVCYHDV
                     INRRGSLPRTSVPAILGHEVAGEVIEVGAGVTSWKVGDRAATLQRLSCGDCAVCRSGR
                     PSLCKHDNRFFGEELAGGYATKMVAPIAGIGRVPDGMSWAVAATVCCTAGTAVHVVRT
                     RGRVKKGETVLVTGASGGVGLQTVQLARLDGAHVIAVTSSADKVEALHNAGAHEVVVS
                     PDLDFSRQVRKLTGGDGVQVAIEIVGSATFAQTLKSMMPGGRVVVVGNLESGTIDLNP
                     GLVIVKELEIIGAYATTQQELETALQLTHAGLLTPFVTEVLPLREAASAHVRLENRKV
                     AGRLVLSPDI"
     gene            3701..4945
                     /locus_tag="VFV99_04475"
     CDS             3701..4945
                     /locus_tag="VFV99_04475"
                     /inference="COORDINATES: protein motif:HMM:NF020129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX5058590.1"
                     /translation="MKIRVGIDAMGVAVPRRYIDIEDLARARGVEPAKYTAGLGAREM
                     AVAEPGEDTVSLAAEAARRALAVAKLDGNDLGLLVVGTETGVDHSKPVASYVHGLLDL
                     PRTMRVYDTQHACYGGTAGFMTALEWIASGSAAGKSALVICSDIARYGINTAGEPTQG
                     AGAVAMVISDSPGMLEVDVGISGTSSTHVHDFWRPLGLREAQVDGHYSVQCYLDALAG
                     AYRGWKQQAIARDIVRGGGDHRDIVSEKLARICYHVPFCKMAKKAHLHVRQCDLEDTR
                     AAWDSAAEEQKAAVSFKQQVEPSLAVCARVGNIYTGSLYLALAGLLDSQAPALSGKRI
                     GLFSYGSGCTSEFFSGIVAARAAERVAAAPLAEVLAGRERIPIAEYEKIMALTAPLTG
                     TPHAGAFRYSGTVEHRRQYTRA"
     gene            5100..6173
                     /locus_tag="VFV99_04480"
     CDS             5100..6173
                     /locus_tag="VFV99_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5058591.1"
                     /translation="MIATAEAALTPVDGFGSNPGALDMYEYVPANLPSGRPLVVVMHG
                     CTQMASGMEAAGWNALADKYQFAVLYPQQRNANQQLGCFTWYAASDIDRDAGEAQSMV
                     QMVDKMIAMHGIDKNRVYATGLSAGAAFTAVLLAAYPDRFRAGSIMAGVPYKCATDVS
                     SGSSCASSGVMKTPEQWGALVRDAFPSFTGTYPRVQIWHGSADHTVATANATELVNQW
                     TNVWGTDQTADATEMISTAMRTQYKAGDKVAVELYLIAGMGHAIATGSDAIGDCPATS
                     GAFFTDEKICSTLRAAEFFGLLGSGSGSGSDGDGDGNGDGNGDGNGSDNDASSGGCSA
                     GGNAGLAVIAAFALVGFVRRRRS"
     gene            6292..6837
                     /locus_tag="VFV99_04485"
     CDS             6292..6837
                     /locus_tag="VFV99_04485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5058592.1"
                     /translation="MRFNPTAYTVRGEHSLVVAFLDLKGFAAQSARITDREIADGIDG
                     YYSRVAERVTKAGGYVVKFIGDGALIVFRSEAADMAIETFLALKSELDDWLEQLGWSC
                     TLTIKIHVGDVIVGPFGGRLDVIGREVNTTAMLDSTGVALSVPAFRALGPEMRQRFKK
                     HTPPVTYIRHEDPRRFRYRPA"
     gene            complement(6834..7478)
                     /locus_tag="VFV99_04490"
     CDS             complement(6834..7478)
                     /locus_tag="VFV99_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5058593.1"
                     /translation="MLLRETFPAGPFECNCTVLACADTKDAVVIDPGGETDRIAEIIK
                     AYDLRVRSIIHTHAHLDHIYCTRDVKEEHGGEICLHKGDAFLYDGFAMQAAMFNWKPR
                     DTLPVERWIQDNDTIVLGKRKLSVIHTPGHTPGSVCFEIVDPTLGDPLMFSGDTLFRR
                     SIGRTDLPGGDSALIQRSIKERLYTRSLDTVVIPGHGPTTKLGDEARGNPFVRR"
     gene            complement(7478..7915)
                     /locus_tag="VFV99_04495"
     CDS             complement(7478..7915)
                     /locus_tag="VFV99_04495"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7784..7786),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5058594.1"
                     /translation="MLSEGELAPDFAATAHDGTHVKLSDFAGQYVLLWFYPMADTPGU
                     TIEGNGFRDRIQEFSAKNVVVLGASYDDPAANKAFADKFKYPYKLLSFSKDDGKAYDA
                     YDASADQYARRISYLIGPDRKIVKAYPKVKPAEHPSQVLADVP"
     gene            7993..8655
                     /locus_tag="VFV99_04500"
     CDS             7993..8655
                     /locus_tag="VFV99_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5058595.1"
                     /translation="MKTRSLPKRALVGFGIAMVAALVFVLLMKEVLEGNADAFDRRWA
                     LAIHDRLATPILTWFLIGVTYVGSGWGVVAVIAVMTLALYRQHHWRTGLILLCDALAA
                     EVLMRILKAYVERPRPTLFDEITRPETWSFPSGHALTAMAVYGGIGAVFITLYPARRL
                     PIIIATAVWVFLIGFSRVYLGVHWPTDIVAGWAAGVPLLIVTVRLLHRKERLKIGRPL
                     PG"
     gene            8672..>8928
                     /locus_tag="VFV99_04505"
     CDS             8672..>8928
                     /locus_tag="VFV99_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5058596.1"
                     /translation="MSVSSVRNQLRSQLRESVSTAILEAAEDLIAAKGLQGAPLLQIA
                     KKAGVAVGTLYNYFEDRDALIRALFEMRRATLWPQLRAAVA"
BASE COUNT         1490 a         2866 c         2983 g         1589 t
ORIGIN      
        1 gaagcgaacg acgtgatcgt tctcgcccac acgcggagca gcgcgccggt cgcgtatgag
       61 gtcgcgacgc gcctcggcct cccgctcgaa ctgttcgatc acggcagtca tgtcgccgac
      121 aagaccgtgc tcttgatcga cgatggcgat gacgcccgag ggatggtgcg ctcgatcagc
      181 gagctgcgcg aacgcgctgc cgtgatggtc gttgcggcca ccgcggtcgc atcaccagcg
      241 gtctacacga tgcttcgcac ggccgccgat cacgtgtcgt gcatcctgtc gccgcagcac
      301 atctactcgg tgcaggcgtg gtttgccgac ttcgagccgc caaacgacga ggagatccag
      361 cagttactgg ccgcggcggc ccagaatctc ttgctattgc gacgcagcaa ttttctgacg
      421 tccggcgttg acacgtagcg ttaccgtaca tagggtacct accgaacggt cggtaggtga
      481 ctatgcactc gctcgacgtg ttacgaaagc tcggtgaatt cgcggtcctc cagcgaaagt
      541 ccgaagctgc gtcggatcct cgaacgcgct gctcgattga ttttcgagaa gggctacgag
      601 ggcacgagca tgcaggacat cgccgatgcc tgcgggctca cgaaggctgg gctgtatcac
      661 cacgtggcaa cgaaggaagt gctcctgatc gagatcatgg agtacgggat ggatctgttc
      721 gaggaggttg tcctcgcgcg ggccgagccc atccgcgatc cgctcgagcg cttgaagaag
      781 acgatggcgt gcaacatcga gctcgtcacg acggactctt cgaaagaagt gacgatcatc
      841 ctgcacgagc atcagacgct caccggcgaa gcgcagaaga agatcaacgg tcgcaagaag
      901 cgctacgtgc ggttcttgga ggatgcgttc aagcaggcga tggccgccgg gcagattcgc
      961 aacgtcgatc cgacgctcgc gtcgttctcg ttcctcggta ccgtgttgtg gacgtacaag
     1021 tggtacaggc cggatggccg gctcacacca cagcagctct ccgacggcac catcgatctg
     1081 ttcttcaacg ggctcgttgc ctaggcggtg actcgtgacc cggtgggttc gcctcgacga
     1141 ggcggccgcc gcggtgcccg atggcgcgtg gatcgcgccg ggcggcttca tgctcggtcg
     1201 cgcgccgatg gcgctgatct tcgcgctgat ccgcgcggag aaacgactgc gcgtgatctc
     1261 gctgccgaac ccgctcccgg cggagatgct cgtggcgaca aatcttgccg cgcgcgtcga
     1321 gctgttgttt gcggcgctga cgctcgcgaa ccgcgtgcga ccgatgcctg cgctcaagcg
     1381 cgcgatcgag cgcggcgagg tgccctgggc cgagcacgac ggctatcgga tcgtgcagcg
     1441 gcttcgcgcc gcagccatgg ggctgccatt cctgccggca cccgacgccg atgtgtccga
     1501 gctgtcgacg ctcgagccac cgtcgtacac gctcgatccg tttaccggag cgcgcgtacc
     1561 ggtcgagcgc gcgtttcatc ccgacgtcgc cctcgtgcac gcgcacgcgg ccgatgacgc
     1621 cggcaacctc tacatcgaag atccgacgac ggacctgctc gtcgtcggcg ctgcgcatcg
     1681 cgtgatcgcg accgccgagg tccgcgtgcg gcaactgacg cgcgtcaccg tgccggcgtt
     1741 tcaggtcgac gtcgtcgccg aggcgacggg tggtgcgtgg ccgacgggct gcgtcggtct
     1801 ctatccacac gacgagaagg cgctgctcgc gtatctcgcg gcatcggaag cgggcgaggg
     1861 cgccgactgg atccagtcgg tgttgcaagg acgcatcccg ctgcgaggtg ctgcgtgatc
     1921 acgcccgccg agcggctcgt cgcgcagatg gctggcgaga ttcacgacgg cgcggtcgtc
     1981 gcgaccggtg tcgcgtcgcc acttgccgtg ctcgcgattg ccgtcgcccg cgcgacccat
     2041 gcgccacggc tgacatacat cgcgtgcgtc ggctcgctga acccgaacgt cacgaggctg
     2101 ttcggttcga gcgaagacct cgcgtacctc gacgggcgcg aagccgagat ctcgattcct
     2161 gatctgttcg atcacgcgcg gcgcggccgc gtcgacacgg tgttcttcgg cgcagtcgaa
     2221 gtcgatggcg aaggccgcac gaacctgtct gcggctgggc cgctcgcgag cccgaagatc
     2281 aagttccccg gcgtcgtcgg cgcatgctcg ctgcgccgct ggtgcaagaa tccggtgctt
     2341 gtcgtgccgc ggcagaacgc gcgctgcttg gttccgcgcg tcaacgtgac gagtaccgag
     2401 gacgttcggc gtacgcgcct gctcaccgac ctcgcggtgt tctcgatcgg cgagggcggc
     2461 gcgatcctcg aagcgattgc accgcatgcg tcgcttgccg acgtgcgcgt gcgcacgggc
     2521 ttcgcgtacg gcgaggcggc gccgatcgca cacttcaccg atcccgacgc ggacgtgctc
     2581 gctgcgatcc gccgcatcga tcccgacaac ctgcgtgacg agctggtcgg ccagctcaac
     2641 tgaaagagga tgccatggaa gcggtagtgc tctcacaatt cggctcggcg cattacctcg
     2701 cgatgcaatc ggttcccgat ccgcatccgg gacccggcga agtgctgctg cgcgttcgcg
     2761 catgcggcgt ctgctatcac gacgtgatca atcgccgcgg cagcctgcca cgcacgtcgg
     2821 tgccggcgat cctcggccac gaagttgccg gtgaggtgat cgaagtcggt gccggtgtga
     2881 cgagctggaa ggtcggcgat cgcgccgcga cgctacaacg actgtcgtgc ggtgactgcg
     2941 cggtgtgccg atccgggcgt ccgagcctct gcaagcacga caatcgcttc ttcggtgaag
     3001 agctcgcagg cggctacgcg acgaagatgg tcgcgccgat cgctggcatc ggtcgcgtgc
     3061 ccgacggcat gtcgtgggcc gtcgccgcga cggtgtgctg tacggcaggc accgcggttc
     3121 acgtcgtgcg aacgcgcggc cgcgtgaaga agggcgagac cgtgctcgta acgggtgcga
     3181 gcggcggcgt tggcctgcag accgtgcagc tcgcgcggct cgacggcgca cacgtgatcg
     3241 cggtgacgtc gagcgccgac aaggtggaag cgcttcacaa cgcaggtgct cacgaggtcg
     3301 tcgtgtcgcc ggacctcgac ttctcgcgcc aggtgcgcaa gctcaccggc ggcgatggcg
     3361 tgcaggtcgc gatcgagatc gtcggcagcg cgacgttcgc gcagacgctg aagtcgatga
     3421 tgcccggcgg tcgtgtcgtc gtcgtcggta acctcgagtc gggaacgatc gatctcaatc
     3481 ccggcctcgt catcgtgaag gagctcgaaa tcatcggcgc ctacgcgacg acacagcagg
     3541 agctcgagac cgcgctccag ctcacgcacg ccggtctact caccccgttt gtcaccgaag
     3601 tgctcccgct gcgcgaagcc gcgagtgcac atgttcggct cgagaatcgc aaggttgcgg
     3661 gtcgcctcgt gctcagcccc gacatctgaa aggaacgcat atgaagattc gagttggaat
     3721 cgacgccatg ggcgtggctg tgccccgccg ctacatcgac atcgaagacc tcgcgcgcgc
     3781 gcgcggcgtc gagccggcaa agtacacggc ggggctaggt gcacgtgaaa tggcggtcgc
     3841 ggagccgggc gaagataccg tgtcgctcgc agccgaagcg gcgcgtcgcg cactggcggt
     3901 cgccaagctc gacggcaacg acctcggcct gctcgtcgtc ggcaccgaga ccggggtcga
     3961 tcactccaag cccgtggctt cgtacgtaca cggtttgctc gacctgccgc gcacgatgcg
     4021 cgtctatgac acccagcacg cctgctacgg tggcaccgcc ggtttcatga ccgcgctcga
     4081 gtggatcgcg tcgggctcgg ctgctggcaa gagcgcactc gtgatctgct ccgacatcgc
     4141 gcgctacggc atcaacaccg ccggcgagcc aacgcaaggt gcaggcgcgg tcgcgatggt
     4201 gatcagcgac agccccggca tgctcgaagt cgacgtcggc atctcgggca cgtcgagcac
     4261 gcacgttcac gatttctggc gtccgctcgg tctgcgcgag gcgcaggtcg acggccacta
     4321 cagcgtgcag tgctatctcg acgcgctcgc cggtgcgtat cgcggctgga agcagcaagc
     4381 gatcgcgcgc gacatcgtgc gcggcggcgg cgatcatcgc gacattgtca gcgagaagct
     4441 cgcgcgcatc tgctaccacg tgccgttctg caagatggcg aagaaggcgc acctccacgt
     4501 gcgccagtgc gatctcgaag acacgcgcgc agcgtgggac tcggcggccg aagagcagaa
     4561 ggcagcagtg agcttcaagc agcaggtcga accgtcgctc gcggtgtgcg cgcgcgtcgg
     4621 caacatctac accggctcgc tctacctcgc gctcgcggga ttgctcgata gccaggcgcc
     4681 cgcgctctct ggcaagcgca tcggcctgtt ctcgtacggt agcggctgca cgagcgagtt
     4741 cttctccggc atcgttgccg cgcgcgccgc tgaacgtgtc gctgccgcgc cgctcgccga
     4801 agtgcttgcg ggccgcgagc gcatcccgat cgcagagtac gagaagatca tggcgctgac
     4861 tgcaccactc acgggcacgc cacacgcggg cgcgttccgc tacagcggta ccgtcgagca
     4921 tcgccggcag tacacgcgag cctgatggca cgcgccaaca ggcgcggttc gatacgtgaa
     4981 tgtaggtcta ctgaaagaat ccgcgacata gctctcgcga tcggtcggca tatgcggtgc
     5041 agtcggctga ccgcggaggg cccatgcgtc tcatctcgag cttcgttgta ctcgccgcga
     5101 tgatcgcaac cgcggaagcg gcgctgacgc cggtcgatgg ctttggcagt aacccgggcg
     5161 cgctcgacat gtacgagtac gtgccggcca acctgccgag tggtcggccg ctcgtcgtcg
     5221 tgatgcacgg ctgcacgcag atggcatcgg gcatggaggc ggcgggctgg aatgcgctcg
     5281 ccgacaagta tcagttcgcg gtgctctacc cgcagcagcg caacgcgaac cagcagctcg
     5341 gctgcttcac ctggtatgcc gcgagcgaca tcgatcgcga tgccggcgaa gcgcagtcga
     5401 tggtgcagat ggtcgacaag atgatcgcga tgcatggcat cgacaagaac cgcgtctacg
     5461 cgaccggtct ctctgccggc gccgcgttca ccgccgtgct gctcgccgcc tatcccgatc
     5521 gcttccgtgc gggcagcatc atggccggcg tgccatacaa gtgcgcgacc gacgtgtcgt
     5581 cgggatcgag ctgcgcgtcg agcggcgtga tgaagacacc ggagcagtgg ggcgcgctcg
     5641 tgcgcgacgc attcccgagc ttcacgggca cgtatccgcg cgttcagatc tggcacggct
     5701 cggccgacca cacggtcgcc accgccaacg caaccgagct cgtcaaccag tggacgaatg
     5761 tgtggggcac agatcagacc gctgacgcga ccgagatgat cagcaccgcg atgcgcacgc
     5821 aatacaaggc gggcgacaag gttgcggtcg agctctacct catcgccggc atgggccacg
     5881 cgatcgcgac tggctccgac gcgatcggcg actgtccggc gacgtccggc gcgttcttca
     5941 cggacgagaa gatctgctcg acgctgcgcg cggccgagtt cttcggcctg ctcggcagtg
     6001 gcagcggtag tggcagtgac ggcgacggtg acggcaacgg cgatggcaac ggtgatggca
     6061 acggcagcga caacgatgcc tcgtcgggcg gctgcagtgc gggtggcaac gcggggctcg
     6121 cggtcattgc agcttttgcg ctcgtcggat tcgtgcgccg gcgccgttcg taactgcgca
     6181 gcgaccggct tcacggtacg cttggcgtaa cagcgggact tcgggcccgt gactctcctc
     6241 cttactgtcg gacatccggg ccgacgctgg attcaggaac caggagaagc catgcgtttt
     6301 aatccgaccg cgtacacggt gcgcggcgag cactcgctcg tcgtcgcctt cctcgatctc
     6361 aaagggttcg ccgcgcagag cgcgcgcatc accgatcgcg agatcgccga cggtatcgac
     6421 ggctactact cgcgcgtcgc cgagcgcgtc accaaggccg gcggctacgt cgtcaagttc
     6481 atcggcgacg gcgctctgat cgtgtttcgc agcgaagcgg ccgacatggc catcgagacc
     6541 tttctcgcgc tcaagtccga gctcgacgac tggctcgagc agctcggctg gagctgcacg
     6601 ctcacgatca agattcacgt cggtgacgtg atcgtcggcc cgttcggcgg ccggctcgac
     6661 gtgatcggcc gcgaggtcaa cacgacggcg atgctcgact cgacgggcgt cgcgctatcc
     6721 gtgccggcgt ttcgcgcgct cggaccggag atgcggcagc gcttcaagaa gcacacgcct
     6781 ccggtcacgt acatccgcca cgaagatccg cggcggttcc gttaccgccc ggcttagcgg
     6841 cgcacgaacg gattgccgcg cgcctcgtcg ccgagcttcg tcgtcgggcc atggcccgga
     6901 atcacgacgg tgtcgagact ccgcgtgtac agccgctctt tgatggagcg ctggatcaat
     6961 gcgctgtcgc cgccgggcag gtcggtgcga ccgatgctgc gccggaacag cgtgtcgccg
     7021 gaaaacatca gcgggtcacc caatgtggga tcgacgatct cgaagcagac cgatccgggc
     7081 gtgtgacctg gcgtgtggat cacgctgagc ttccgcttgc cgaggacgat cgtgtcgttg
     7141 tcctggatcc agcgttcgac cggcaacgtg tcgcgcggct tccagttgaa catcgccgcc
     7201 tgcatcgcga agccgtcgta caggaacgcg tcgcctttgt gcagacagat ctcgccgccg
     7261 tgctcttctt tgacgtcgcg cgtgcagtag atgtgatcga ggtgcgcgtg cgtgtggatg
     7321 atcgatcgca ccctgagatc gtacgctttg atgatctccg cgatgcggtc ggtctcgccg
     7381 cctggatcga tcacgacggc gtctttcgta tcggcgcacg cgagcacggt gcaattgcac
     7441 tcgaacggac cggcgggaaa cgtctcgcgc aacaacatca aggcacgtcg gcaagcacct
     7501 ggctggggtg ttcggcgggt ttcactttgg gatacgcctt cacgatcttg cggtccggac
     7561 cgatcaggta cgagatccgg cgcgcgtact ggtcggcgga tgcgtcgtac gcgtcgtagg
     7621 ccttgccgtc gtctttcgag aagctgagca gcttgtacgg gtacttgaac ttgtcggcga
     7681 acgctttgtt tgcggcgggg tcgtcgtagc tggcaccgag cacgaccacg ttcttggcgg
     7741 agaactcttg gattcgatca cggaatccat tgccttcgat cgttcaacca ggtgtgtcgg
     7801 ccattggata gaaccagagc agcacatatt gaccggcgaa atcggacagc ttcacgtgcg
     7861 tgccgtcgtg cgccgtggct gcaaagtctg gagcgagctc gccttcggaa agcatgcctg
     7921 gagcgtacgc caagtcggag gcagacgttg cgagttttcc tgaaggcacg ttcactgcac
     7981 gatgtactga ccgtgaagac ccgatcgctc ccgaagcgcg cgctcgtcgg gttcgggatc
     8041 gctatggtgg cggcgctggt cttcgtcctc ctcatgaagg aggtgctcga gggcaatgcc
     8101 gacgcattcg accggcgatg ggcgctggcg atccatgacc ggctcgcaac gcccatcctc
     8161 acgtggttct tgatcggcgt gacgtacgtc ggcagcggct ggggcgtcgt cgcggtgatc
     8221 gcggtcatga cgctcgcgtt gtatcgccag catcattggc ggaccgggct catcctgttg
     8281 tgcgacgcgc tcgcagccga ggtgctgatg cgcatcctca aggcctacgt cgagcgaccg
     8341 cggcctacgc tattcgacga gatcacgcgc cccgagacct ggagcttccc gagcggtcac
     8401 gcgctgaccg cgatggccgt ctacggaggt atcggagcgg tcttcatcac cctgtatccg
     8461 gcacgccgac tcccgatcat catcgcgacc gcggtgtggg tcttcttgat tggcttttcg
     8521 cgcgtgtact taggcgtgca ctggccgacc gacatcgtgg ctggttgggc cgccggtgtt
     8581 ccattgctga tagtcactgt tcgcctgttg caccgaaagg agcgcctgaa aatcggtcgc
     8641 ccgctacccg gatgaatgta tattcacctc cgtgagcgtg agttcagttc gcaaccagct
     8701 ccggtcgcaa ttacgcgagt cggtgagcac ggcgatcctc gaagccgccg aggacctgat
     8761 cgccgcgaag gggttgcagg gcgctccgct gctccagatc gcgaagaagg ccggggtcgc
     8821 ggtcgggacg ctctacaact acttcgagga tcgcgatgcg ctgatccgcg cgctgttcga
     8881 aatgcgccgg gcgacgctgt ggccgcagct ccgtgccgct gtcgcgag
//
LOCUS       DASUFX010000117         7805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1548467, whole genome shotgun sequence.
ACCESSION   DASUFX010000117 DASUFX010000000
VERSION     DASUFX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548467"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFV99_04510"
     CDS             <1..1007
                     /locus_tag="VFV99_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058597.1"
                     /translation="VRRKTGGRPRLVLAVDKQPMQLPLGYTIVDLEDILAPGAYLLDI
                     VDAKGELLGLTIGASIGQLRNGDASGGDEDGETPTMAPLLPSTGSETRLVLEANVRAT
                     QMAFQHNQKTLELGLRMAETLRDGVHVLADAQAEWIKSLSSARGFFRNAAPPPPALPS
                     PAPSKDEDEDDEDDGYEEGPSSLIETVGPLVAMAAEKIATTIMQIKMGGGGASTDVQF
                     GDLFDWRRPAQRNRAAREKAERPQLDAAEAAAPPLATARANTRTLEAMVEADPKLKMH
                     LFAIMAALSEEERRHAQAAAKILSDEDRLAWLNSLKAMSVSDAVQQIRGLIAQPAAQT
                     "
     gene            1294..2232
                     /locus_tag="VFV99_04515"
     CDS             1294..2232
                     /locus_tag="VFV99_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5058598.1"
                     /translation="MSDAVQEHVVSMRERGLRSNTVTRAEYHLRSFFHLDAEEDGKTI
                     PFAKSGGLLEDLSPRRCEALYQQLAKTVSVDTHRNALAAAKTFGKCCVDRGWIRINPA
                     VGVKPFGQRNRGKKQLRIDEARRLVDLCIKKANAGDDAAVAVLTSLMLGLRASEVTDR
                     VVRDLDDEGRLLWIEFGKTKRSRRTLEVPAMLRPYLLALAKGRAPDEQLITRKQTKRG
                     NKRDRHWLLHWLHAYCDEASIPSVCSHSLRGLHATLAMEAGQTSHAVASALGHSTPAV
                     THAHYIEAGAKRRASTRRVTGKLGDSAASMRLVGNG"
     gene            complement(2300..2381)
                     /locus_tag="VFV99_04520"
     tRNA            complement(2300..2381)
                     /locus_tag="VFV99_04520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2345..2347),aa:Leu,seq:cag)
     gene            2586..4205
                     /locus_tag="VFV99_04525"
     CDS             2586..4205
                     /locus_tag="VFV99_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5058599.1"
                     /translation="MAKLIVISGEERQEFELAAFNTIGRHPDNTIQILDRIISKEHAQ
                     IQRSADGRFLLRDLRSLNGTFLRGERVSDHYLADGDEFTMGSTRIVFVDKPKADDALQ
                     RVTIAPGLTESHIRGRIQANTGDFMPERQIADEKILRRDYERLRIGHELARAVGSELD
                     LDKLLPKILDKAFELVGADRGVILLVDEKGELAPRFVKTRSGKSDPNIVLSKTVMAEV
                     TSNKAAVLSSDATMDARFSGAHSIIMQGIRSTMTLPLLYANELLGIMHLDSLFTSNAF
                     TEKDLQICTGMAQQAAISIQNARLASRIEREAQTRAQISRLIPPSVVEQVVKGELTIE
                     KGGRLTECTMLFSDIRGFTTMSDGRPAQEIVNTLNEYFEVMVDILFKYSGTLDKFVGD
                     EIIGLFGAPIAIDDAPFKAVACGIAMLQGLEEFNRTRASENQAPIRIGIGINTGNVIT
                     GSIGSTRALQYTAIGDAMNVASRLVNVAGSGEIIISENTYRHVAGRVEAAQLPPVKVK
                     GKAEELKVYRVTGLRNLQTPVPSDWSGPTNG"
     gene            4308..5504
                     /locus_tag="VFV99_04530"
     CDS             4308..5504
                     /locus_tag="VFV99_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058600.1"
                     /translation="MSDEAPPAGSSDSVRHARDLGLNVLVVGVALIALQNFPPLPIRG
                     AQLSDGIAGLGAPLVLATTLRIGGWSALVPSRAATVAAVGYALFACVSFATAGGSAMR
                     LLGYVWLVGFGFAVSVAGQDADVAQRLRRALIVAALIGAVTGLVGAVLFWTGKPTGLL
                     NIAGDLVPGNYPRIRGTLGRANALAGLLATGLLLVGDVPRRWRVPVAIVLAVALVFTF
                     SRSWIALGASGAVWWLALTPKHTRMRDIAAVLVVLATIAVMLAVSWLEIRVDPTRPFA
                     TTIGDGVGTRWIHLNDALSTISAHPLGVGPGRAATYNGWDAHFSPINIAAVIGIPAAI
                     AFVVLFALALVRSIRAARDGDIVFRGVAAALVLFGIDALARDIEDQRALWVLLGLALV
                     GRVRRR"
     gene            complement(5512..6333)
                     /locus_tag="VFV99_04535"
     CDS             complement(5512..6333)
                     /locus_tag="VFV99_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058601.1"
                     /translation="MAAAAVMREPSAPQPPPPLDRSVFARDPNGQLSEDMLQKILGTP
                     IELDLPSRVGILPIITATDWRGPSPDWDRVPPGVSPLVARLRKDAAFSLVTEMMPIPS
                     GSLGMEALREAAARYRLRYVILYREVLAKKSHQNVWTWGYATAVGALFLPGKQHEVYG
                     YIEATMFDVKTGLLMFTTRRAIMAEKRSNEWRTADKLAQLASNAVTKFAPDLAVDFLL
                     DVRRFAHAAVAENERRFGAQTVSEVGSSGPAIVQVPPVSGELVTVPPSSGTVAHD"
     gene            6580..6981
                     /locus_tag="VFV99_04540"
     CDS             6580..6981
                     /locus_tag="VFV99_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058602.1"
                     /translation="MTKMKLALVLCGCLIGGVAAAAPKWDANGDGTISAQEKAQRHEE
                     MKAKREEMKAKMLAKFDTNKDGKLDQTERGAMRETFAIDAFKRLDTNGNGSLSLDEFK
                     AGQHFMGKHHGRFGKGGARGFHRGAFKTRSK"
     gene            7293..>7805
                     /locus_tag="VFV99_04545"
     CDS             7293..>7805
                     /locus_tag="VFV99_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058603.1"
                     /translation="MARRVPPLAAAIVLVAAGLVATAWSTRRAVNDAFDAVGEGQAFT
                     IEQGVRADLADLGGPPGNEELAGILGEHAQEGLVYLATLDGRGRVQAAAGTPLGGSAG
                     RADRVGVHIEHVGDRVRIEVRTMFRRAWGPGRGSLWVAMEIEPVQAAELRSAASRTLA
                     IGLVGAIMLLG"
BASE COUNT         1333 a         2451 c         2686 g         1335 t
ORIGIN      
        1 gggttcgtcg caagaccggc ggtcgaccgc gcctcgtgct tgcggtcgac aagcaaccga
       61 tgcagctgcc gctcggctac acgatcgtgg acctcgaaga catcttggcg ccgggcgcgt
      121 acctgctcga tatcgtcgac gcaaaaggtg agctgcttgg acttacgatc ggcgcctcca
      181 tcgggcagct tcgcaacggc gacgccagtg gcggcgacga ggacggcgag acaccgacga
      241 tggcaccgct tctgccatcc actggcagcg agacgcggct cgttctcgaa gcgaacgtgc
      301 gtgcgaccca gatggcgttc cagcacaacc agaagacgct cgagctcggg ctgcgcatgg
      361 cagagacgct gcgcgacggc gtccatgtcc tcgccgatgc acaggcagag tggatcaagt
      421 ctctctcttc agcgcgcggt ttctttcgca atgccgcgcc accgccgccc gcattgccgt
      481 cgccggcacc ctccaaggac gaagacgagg acgacgaaga tgacggctac gaggaaggac
      541 cgtcctcgct catcgaaacg gttggtccac tggtcgcgat ggcagccgag aagatcgcga
      601 cgacgatcat gcagatcaag atgggcggtg gcggggcgtc cacggacgtg cagtttggcg
      661 acctattcga ttggcgtcga ccggcgcagc ggaacagggc ggcgcgcgaa aaggcggagc
      721 gacctcagct cgatgcagcg gaggctgctg caccgccgct cgcaacagcg cgcgccaaca
      781 cgcgaacgct cgaagcaatg gtcgaggctg acccgaagct gaagatgcac ttgttcgcga
      841 ttatggcggc cctgtccgag gaggagcgcc gtcacgcgca ggctgctgca aaaatcctgt
      901 ccgacgaaga ccggctcgca tggctcaatt cgctgaaggc aatgagcgtc tcagatgcgg
      961 tgcagcagat ccgcgggctg atcgcgcaac cggcggcaca gacatgacga gatctcgtgc
     1021 gctcaccgtc gaggacatcc ctgatctcgt cgacgccatc gtgctcgcgc tcgaacagcg
     1081 tggcatgctc cccggcggga atccgtcgcc gggattggaa gacacccgat gcgacagaat
     1141 ggagagagag ttcatggacc gtacaggcac cgcaaccgat ggcgtgtcgt gctccgtgga
     1201 ggaggcggcc gccaagaggt tgtctcgttt tcgtcggaag ccgaagccag gcagtacaag
     1261 gctgccctta gcaagcagat cgaaggccgc acggtgagcg atgccgtgca ggagcatgtg
     1321 gtctcgatgc gtgagcgcgg attgcgtagc aacaccgtga cgcgtgccga ataccacctg
     1381 cgcagcttct tccacctcga cgccgaggag gatggcaaga cgattccgtt cgccaagagc
     1441 ggcggcttgc ttgaagacct cagtccccgc cggtgtgaag ccctgtacca gcagctcgcg
     1501 aagacggttt ccgtcgacac gcaccgcaat gcgcttgcag ctgcgaagac gttcggcaag
     1561 tgctgtgtgg accgcggctg gatccggatc aatcccgcgg tcggcgtgaa gccgttcggt
     1621 caacggaatc gggggaagaa gcagctacgt attgacgagg cacgcaggct cgtcgatctc
     1681 tgcatcaaga aggcgaatgc cggtgatgat gcagcggtcg cggtgcttac gtcgttgatg
     1741 ctcggccttc gtgcctccga ggtgaccgat cgagtggttc gcgatctgga tgacgaaggg
     1801 cgtctgctct ggatcgagtt cggcaagacg aagcggtcgc ggcgcacgct ggaggttccg
     1861 gcgatgctgc ggccgtactt gctcgcgctc gcgaaaggga gggcgccgga cgagcagctg
     1921 atcacccgga agcagaccaa gcgcggtaac aagcgagacc gccactggct tctgcattgg
     1981 ctgcacgcgt actgcgacga ggcttcgatc ccgagcgtct gctcccacag cttgcgcggc
     2041 cttcacgcaa cattggcgat ggaggcgggc cagacctcgc acgcggtcgc gagcgcgctg
     2101 ggccacagca cgcctgcggt cactcacgcc cactacattg aagcgggcgc gaaacggcgg
     2161 gcgagcacgc gccgggtaac gggcaagctc ggcgattcgg ctgcgagcat gcgcctggtg
     2221 ggtaacgggt aatgagccca cctgttaccc gcgcgtcgaa aagccttttg gggcttttgc
     2281 aacgccgcgt gtttcttcgt gcgatcgggg ggactcgaac ccccacggtg ttacccactg
     2341 gaacctgaat ccagcgcgtc taccaattcc gccacgatcg cttaattgtc agtcccctca
     2401 gcgcaggctc cagggaggca gactgatgcc acaaaacacg ggagatcaca agaccgcagg
     2461 agggcctgtg ggagccagaa acctacccat tttccgagtg tgggcaagga gttggtcggc
     2521 ccggtcgctt tactcggttg ggcctgttcc gtaccatcgt tgaagcgtcc ctttcgagct
     2581 tcgcgatggc aaagctgatc gtcatttcag gtgaagagcg gcaggagttc gagctcgccg
     2641 ccttcaatac gattggtcgg catcccgaca acacgatcca gatcctggat cgcatcatct
     2701 cgaaggagca cgcgcagatt cagaggtcgg cggacggccg gttcttgctg cgtgacttgc
     2761 gctcgctcaa cggcacgttc ctgcgcggcg agcgcgtcag cgaccattat ttggcggacg
     2821 gcgacgagtt cacgatgggc tcgacccgca tcgtgttcgt cgacaagccg aaggccgacg
     2881 atgcgctcca gcgcgtcacg atcgccccgg gcctgaccga gagccacatt cgcggccgta
     2941 tccaggccaa caccggcgac ttcatgccgg agcgccagat cgcggatgag aagatcctgc
     3001 ggcgcgacta cgagcgtctg cgcatcggcc acgagctcgc gcgcgccgtc ggcagcgagc
     3061 tcgatctcga caagctgctt ccgaagatct tggacaaagc gttcgagctg gtcggcgccg
     3121 accgcggcgt gatcttgctc gtcgacgaga agggcgagct cgcgccgcgc ttcgtgaaga
     3181 cgcggtcggg caagagcgat ccgaacatcg tgctctcgaa gaccgtgatg gccgaggtca
     3241 cgagcaacaa ggccgccgtg ctgtcgagcg acgcgacgat ggatgcgcgg tttagcggcg
     3301 cgcactcgat catcatgcag ggcatccggt cgaccatgac gctgccgctg ctgtacgcga
     3361 acgagctgct cggcatcatg cacctcgact cgctgttcac gtcgaacgcg ttcaccgaga
     3421 aggatctgca gatctgcacc ggcatggcgc agcaggccgc gatttcgatc cagaacgcgc
     3481 gccttgcgag ccgcatcgag cgcgaagcgc agacgcgcgc gcagatctcg cgactgattc
     3541 cgccgagcgt cgtggagcag gtcgtcaaag gcgagctgac catcgaaaag ggcggccggc
     3601 tgaccgagtg cacgatgctg ttctccgaca ttcgcgggtt caccacgatg tcggacggtc
     3661 gtcccgcgca ggaaatcgtt aacaccttga acgagtactt cgaggtgatg gtggacatcc
     3721 tgttcaagta cagcggcacg ctcgacaagt tcgtcggtga cgagatcatc ggcctgttcg
     3781 gcgcgcccat cgcgatcgac gacgcgccgt tcaaggcggt cgcgtgcggc atcgcgatgc
     3841 tgcaaggcct cgaggagttc aaccgcacgc gcgcgagcga gaaccaggcg ccgattcgca
     3901 tcggcatcgg tatcaacacc ggcaacgtga ttaccgggtc gatcggttcg acccgcgcgc
     3961 tgcagtacac cgcgatcggt gacgcgatga acgtggcctc gcgcctcgtc aacgttgcgg
     4021 gctcgggcga gatcatcatt tcggagaaca cgtaccgcca cgtcgcggga cgcgtcgaag
     4081 ccgcgcagct gccgccggtc aaggtgaagg gcaaggcgga agagctcaag gtctatcgcg
     4141 tgacggggct gcgcaacctg cagacgcccg tcccgagcga ctggtccggc ccgacgaacg
     4201 gctaacgacg ggcgaagccg cggctgcttg ctgtgccgac gaacggctga gccgccggcg
     4261 tcgccgcgta gtccgcgtgg tcgtgcggcc tcgtgtatcg ttgccgcgtg agcgacgaag
     4321 cgccgccagc cggctcgagc gacagcgttc gccacgcgcg cgacctcggg ctcaacgtgc
     4381 tggtcgtcgg tgtcgcgctg atcgcgctgc agaatttccc gccgctaccg attcgcggcg
     4441 cgcagctctc tgatggcatc gccgggctcg gtgctccgct cgtgctcgcg accacgctgc
     4501 ggattggcgg gtggtcggca ctcgtgccgt cgcgtgccgc gaccgttgcg gccgtggggt
     4561 atgcgctgtt cgcgtgcgtg tcgtttgcga cggccggcgg tagcgcgatg cgcttgctcg
     4621 gctacgtgtg gctcgtcggg tttggttttg cggtgagcgt tgccgggcag gatgccgacg
     4681 tcgcgcagcg cctgcgccgc gcgctgatcg tcgctgcgtt gatcggcgcc gtgaccgggc
     4741 tcgtcggcgc cgtgctgttc tggactggca agccgacggg gctcctgaac atcgccggcg
     4801 atctcgtgcc cggcaactac ccgcgtattc gcggcacgct cggtcgcgcg aacgcactcg
     4861 ccggcttgct cgcgacgggc ctgttgctcg tcggtgacgt gccgcgcagg tggcgcgtac
     4921 cggttgcgat cgtgcttgcg gtggcgctcg tgttcacgtt ctcgcggagc tggatcgcgc
     4981 tcggcgcctc gggggccgtg tggtggctcg cactcacgcc gaagcacacc cgcatgcgcg
     5041 acatcgccgc ggtgctcgtc gtgctcgcca cgatcgccgt gatgctcgcg gtgtcgtggc
     5101 tcgagatccg tgtcgatccg acgcggccgt tcgcgacaac gatcggcgat ggtgtcggaa
     5161 cgcgctggat ccatttgaac gacgcgcttt cgacgatcag tgcgcatcca ctcggcgtcg
     5221 gacccggccg cgccgccacg tacaacggct gggacgcgca cttctcgccg atcaacatcg
     5281 ccgcggtgat cggaattccc gcggcgatcg cattcgtcgt gttgttcgcg ctcgcactgg
     5341 tgcgctcgat tcgcgcggcc cgcgatggcg acatcgtgtt tcgcggcgtc gctgcggcgc
     5401 tcgtgttgtt cggtatcgac gcactcgcgc gggatatcga ggaccagcgc gcgttgtggg
     5461 tgctgctcgg gctcgcgctc gtcggtcgcg ttcggcgccg gtaatcggac ctcagtcgtg
     5521 cgcgaccgtg ccactcgacg gcggcaccgt gacgagctcg ccgctcacgg gcggcacctg
     5581 cacgatcgcc gggccactcg agccgacttc cgagacggtc tgcgcaccga agcggcgctc
     5641 gttctcggcg acggccgcgt gcgcgaagcg gcgcacgtcg agcaggaagt cgaccgcgag
     5701 gtcaggcgcg aacttcgtca ccgcattcga tgcgagctgc gcgagcttgt ccgcggtgcg
     5761 ccactcgttc gagcgcttct cggccatgat cgcgcgacgc gtggtgaaca tgagcaggcc
     5821 tgtcttgacg tcgaacatcg tcgcctcgat gtagccgtac acctcgtgct gcttgccggg
     5881 caggaacagc gcgccgacgg cggtcgcgta gccccacgtc cacacgtttt gatggctctt
     5941 cttcgcgagc acttcgcgat agaggatgac gtagcgcagg cgatagcgtg cggcggcctc
     6001 gcggagcgcc tccatgccga gcgagcccga gggaatcggc atcatttcgg tcacgagcga
     6061 gaatgcagcg tctttgcgta gacgcgcgac gagtggcgac acgcccggtg gcacgcggtc
     6121 ccagtcgggg ctcgggccgc gccagtcggt cgcggtgatg attggcagga tgccgacgcg
     6181 cgacggcagg tcgagctcga tcggcgtgcc gaggatcttt tgcagcatgt cctccgacag
     6241 ctggccgttc ggatcgcgcg cgaacacgga gcgatcgagc ggcggtggtg gttgcggtgc
     6301 cgacggctca cgcatcacag cggcagcggc cattggcgcc gacgccatac gcggacggct
     6361 agcaccacag gcagagacaa agagaacgag gactagtgcg gctcgggtca tttgcgcaac
     6421 caacgagcgt cgacctcgat cacttacgcg gatctcgcat gcggttttcg cagccgacct
     6481 gcgggttccg caggacctac atcggcacgc tgcaatgcga tcgcgtggtt acgggcggca
     6541 cgccggtcgc aaataggggc ggaacaggag gatgtgcaaa tgacgaagat gaagctcgcg
     6601 ctcgtgctct gtggctgttt gatcggtggc gtcgcggcgg ctgcgccgaa gtgggacgcc
     6661 aacggcgacg gcacgatcag cgcgcaagag aaggcgcagc gccatgaaga gatgaaggcg
     6721 aagcgcgaag agatgaaggc gaagatgctc gccaagttcg acaccaacaa ggacggcaag
     6781 ctcgatcaga ccgagcgtgg cgcgatgcgc gagacgttcg cgatcgatgc gttcaagcgc
     6841 ctcgacacca acggtaatgg ctcgctctcg ctcgacgagt tcaaggccgg ccagcacttc
     6901 atgggcaagc atcatgggcg cttcggtaag ggcggcgcgc gtggtttcca tcgcggcgcg
     6961 ttcaagactc gctcgaagta atgggggcag accgggcaga ccccgtttcg gatgccccct
     7021 tttcgtaact gcgcacaacg tagatttgct gggctgggtt ggagcacgtc agtcgcggtc
     7081 acggctgacg tcacaggatt gccgcgctgt catggtgcgc gacgatccgg ggcacgggcg
     7141 cgcgtgatgg ggcggtcact ctgtcacgcg cgcccgcggt cttttgctcg gtcggccagc
     7201 tcccactcat cgagccacgg tcaggctcga tgccgtgttg tcttgtgttg cgtcgatcgc
     7261 gcgcggaggt cgcaagtcgt atgctgctcg ccgtggctcg tcgtgttccg cctctcgctg
     7321 cagcgatcgt gctggtcgcg gcgggactcg tcgcgaccgc atggtcgacg cgacgcgcgg
     7381 tcaacgatgc gttcgacgcg gtgggcgaag gtcaggcatt caccatcgag cagggggtgc
     7441 gcgccgacct cgcggacctc ggtgggccgc ctggcaacga ggagcttgcc gggatcctcg
     7501 gcgagcacgc gcaggaaggt ctcgtgtacc tcgcgacgct cgatggccgc ggtcgcgtgc
     7561 aggcggccgc tggtacgccg ctcggtggaa gcgcgggccg cgccgatcgc gtaggcgtcc
     7621 atatcgagca tgtcggcgat cgcgtgcgca tcgaggtgcg cacgatgttt cgccgcgcgt
     7681 ggggcccggg acgcggctcg ctgtgggtcg cgatggagat cgagccggtg caggcagccg
     7741 agctgcgcag tgccgcgagc cggacgctcg cgatcgggct cgtcggtgcg atcatgctgc
     7801 tcggc
//
LOCUS       DASUFX010000118         5866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_992584,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000118 DASUFX010000000
VERSION     DASUFX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992584"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..525)
                     /locus_tag="VFV99_04550"
     CDS             complement(139..525)
                     /locus_tag="VFV99_04550"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5058604.1"
                     /translation="MGKDADCCDTCRMFGDESKVPEPPPPAPAAHADRIKLVIESRGQ
                     TREVLPHGPHMKVGRGKQCDITIPDTSLSRVQCILFFDERGMFVKDSGSTCGTFVDGR
                     KTSGPAQLQVGTKIFFGDSVVRVVAR"
     gene            complement(544..1002)
                     /locus_tag="VFV99_04555"
     CDS             complement(544..1002)
                     /locus_tag="VFV99_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058605.1"
                     /translation="MPNKEPIPDKQDETLNGDEDCPDAMRGVSLAVKERPGGVALTFT
                     TKDRSNLAELRKGVREAAAYLEFRSKMVQVHKDELESVADSMPALDVSVKDLPRGVMA
                     TIRAEDAKDAHELVAQAHNLKESWDNSACATPNVDISRLQRTRFSPSSAP"
     gene            complement(1149..3497)
                     /gene="pbpC"
                     /locus_tag="VFV99_04560"
     CDS             complement(1149..3497)
                     /gene="pbpC"
                     /locus_tag="VFV99_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX5058606.1"
                     /translation="MDRKAALKARWLRRGLIAAIALNALWLVLIGAVFAVPLPERSSD
                     WSVAVEYRDGQPAYIFLTHDEKWRLPVTLDRVDPKYTAALIALEDKRFYSHVGVDPIA
                     ITRAAWTDIIARRRVSGGSTISMQLARLLEPRPRTIASKLIEMFRAMQLDARLSKREI
                     LENYLSRTPFGRNLEGIESAAWSYFGHGAQHLSPLEIATLLAVPQGPARYSPTKQNAE
                     RLRERRDTILKKLIDAGVFSADDTAAAIAEAAAVAPPTKMRQMPRNAAHAAVWLRDLS
                     PGQVRIRSTLEPGAQALAERVVALHAPELQRKHIYNGAVVVVDHRTREIVGLVGNLDF
                     TDFKHGGQIAMFKRARSPGSTLKPLLYSLAIDRGIALPDYLVADVPVEYGTYRPHNFD
                     GDFMGLVTLRDSLARSLNVPFVELLQKLGVEPFISELQRSGVAPTRAKHGEYGLSLIA
                     GGIELTPLEIASLYATLAENGTYVPLRLVFPEGRSESVPLHLGTDKPGHPQAIYGAPA
                     AWLTKEALSLRDRPDFPKRRDILRGIPPEIHWKTGTSFGYRDAWSVGSGPAYTVAVWT
                     GNVDQKASYELVGSEAAGPLLFDVLEGLADRTRSAVPPSAPADLIEIEVCAYSGHIPT
                     DACTHRVKTRAPLHSVPTEPCPYHQVCRAEGKQPESYVALPSSVTAWLVERNRAVPEV
                     PKACAATDIGAPIMMMPTEGQVITLIPGVPAANQVVPLQVSTRAGTVTWFVDGELVGT
                     AAASERMYWTPTVGKHDVVVSDEAGRKARRKLVVQMGASQLR"
     gene            complement(3476..>5866)
                     /locus_tag="VFV99_04565"
     CDS             complement(3476..>5866)
                     /locus_tag="VFV99_04565"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX5058607.1"
                     /translation="TFAMVAVVDEGILSLTGFKTPDPLTELFAKRALGVETYETIGWT
                     MLHQPAGASSKTGGGDYDEDGSEGGGGALDAGRVQPVKPVALFSGVLPVGPDGKVSIP
                     FHVPTYRGELRIMAVTAGPTKIGRAEAKVTVKDPLVIQVTFPRFVTQGDEMQIPVFMT
                     NMSGGPLDVQVTMNSTAIPIAGLAQPKNAAAPLTFAGKDNGALKIQDGLAETLVFRAN
                     ANMPVGGAKLRVVAKAKGKAGAFEVADEVDIPFLPSGPKERVVQKIKIAPGKLDLASQ
                     PALKGWMPLSEQTTFWLTSNPYGESFDHLKYLIHYPYGCIEQTTSSTRPLLYVGNIVE
                     QVDPQLAQLKIEDMVLSGINRVLSMETPSGGFGYWPGSTEPVEWGTAYATHMLLDAKK
                     AGYAVPEDRLQAVLTWIENRAAERESGHLNTASWHHYDEQAEAYLHYVLALAGKGKKA
                     RILKLIEQIPAAKATGEQLEDLYMLKAALYLAGDRRYAADLKNVDASPVTKDRVNYWS
                     FYSDRRRRGLQLSTFFDLFGNDPAGEPLANRVAEILTAEKYAYYYNTQELVWGITGLG
                     KWVQGRVGKGTAAGTLVADGATIEPRKMKKPTNDKSWSLQRASEYKSLTLDLPQAADG
                     MWLVISSEGVRPNQPYKVGGNGLTVSRTYRNSAGDPIDLSKGEVKLGEVVYVEVEVAN
                     TSGNQIQNLALVDRLPAGFEIENPRLGRSFKADWIEPDDQWQLDFLNMRDDHLEAFGM
                     LQSGASKKVVYTVRAVTSGTYTVPPVEVEAMYDPTLWAREDGGKAVIGGPWTGKLL"
BASE COUNT          935 a         1912 c         1964 g         1055 t
ORIGIN      
        1 aggtgcccgc acgacgggca acgccaccac gtcatgaacg tcgtcgattg accgtcgggg
       61 aatcggctcg tgcgcgtgtg tgcggactcg acgttgcagc agcgcagcgg agcgaaggac
      121 atgcgtcgag tgtaagcgtc agcgcgcgac gacgcgcacc acgctatcgc caaaaaagat
      181 cttggtgccg acttgaagct gcgcgggccc gctggtcttg cggccgtcga cgaacgtccc
      241 gcacgtgctg ccgctgtcct tcacgaacat gccgcgctcg tcgaagaaca gaatgcactg
      301 cacgcggctg aggctggtgt ccgggatcgt gatgtcgcat tgcttgccgc ggccgacctt
      361 catgtgcgga ccgtgcggca gcacctcgcg cgtctgaccg cgactctcga tcacgagctt
      421 gatgcgatcg gcatgcgcgg ccggcgcggg cggcggtggt tccggcacct tcgactcgtc
      481 gccgaacatc ctgcacgtgt cgcaacagtc ggcgtccttc cccatgtgtc gagcgtaccg
      541 tgcttacggc gcgctcgacg gggaaaatct agtccgctgc aggcgcgaga tgtcgacatt
      601 gggcgtagcg catgcgctgt tgtcccacga ctccttgagg ttgtgcgcct gcgccacgag
      661 ctcgtgcgcg tcctttgcat cttctgcacg gatcgtcgcc atgacgcctc tcggtagatc
      721 cttcacggag acgtcgagcg ctggcatcga atcggcgacc gattcgagct cgtccttgtg
      781 gacttgcacc atcttgctgc ggaactcgag gtacgcggcg gcctcgcgca cgcctttcct
      841 cagctctgcg aggttgctgc ggtccttcgt ggtgaacgtg agcgcgacgc ctccggggcg
      901 ttccttgacg gcaagagaaa caccgcgcat ggcgtcggga cagtcctcgt cgccgttaag
      961 tgtctcgtcc tgtttatccg gtatcggctc cttgttcggc atggggtccg cgccggcgga
     1021 ggcggtcaac cagctgatcc cgagcccaac gatcaaagca acgcgcgatg tcatgcaaac
     1081 ctccagtgcg atgacattca cgcgccacgc cacgcgtgga cggtgcatgc gcatcgagca
     1141 gtttcggatc agcgcaattg cgaggcgccc atctgaacga cgagcttgcg acgcgccttg
     1201 cggcccgcct cgtcggacac gacgacatcg tgcttgccga cggtcggagt ccagtacatg
     1261 cgttcgctcg ccgcagcggt accgacgagc tcgccgtcga cgaaccacgt gacagtgccc
     1321 gcacgcgtcg agacttgcag tggcacgacc tgattggcgg cgggcacgcc ggggatgagc
     1381 gtgatcacct ggccttcggt cggcatcatc atgatcggag cgccgatgtc ggtggcggcg
     1441 cacgctttcg gaacctccgg cacggcgcgg ttgcgctcga cgagccacgc ggtcaccgag
     1501 ctgggcagcg cgacgtacga ctcgggctgt ttaccttcgg cgcggcacac ttggtgatac
     1561 gggcacggtt cggtcggcac cgagtgcagt ggtgcgcgtg tcttcacgcg gtgcgtgcat
     1621 gcgtcggtcg ggatgtggcc ggagtacgcg cagacctcga tttcgatgag gtcggcaggc
     1681 gcggacggcg gcacggcgct gcgtgtgcga tcggcgagac cttcgagcac gtcgaacagg
     1741 agaggcccag cggcttccga tccgacgagc tcgtagctcg ccttctgatc gacgttgccg
     1801 gtccacacgg caaccgtgta cgcgggcccc gagcccaccg accacgcatc gcgatagcca
     1861 aagctcgtgc cggtcttcca gtgaatctcg ggcgggatgc cgcgcaggat gtcgcgccgc
     1921 tttggaaagt cggggcgatc gcgaagcgag agcgcttctt tggtgagcca cgcggcaggc
     1981 gcgccgtaga tcgcttgcgg gtggccgggc ttgtcggtcc caagatggag agggacggat
     2041 tcactcctgc cctcggggaa gaccaggcgt agcggcacgt aggtgccgtt ctcggcgagg
     2101 gtcgcgtaga ggctcgcgat ctcgagtggc gtgagctcga tgccgccagc gatcagcgac
     2161 agtccgtatt cgccgtgttt cgcgcgtgtc ggcgcgacgc cggagcgttg cagctcggag
     2221 atgaatggct cgacgccgag cttctgcagt agctcgacga acggaacgtt gagcgagcgc
     2281 gcgagcgagt cgcggagcgt gacgaggccc atgaagtcgc cgtcgaagtt gtgcggccga
     2341 tacgtgccgt actcgaccgg cacatcggcg acgaggtagt cggggagcgc gatgccgcga
     2401 tcgatcgcga gcgagtagag caggggcttg agcgtcgagc ccggcgagcg tgcgcgcttg
     2461 aacatcgcga tctgaccgcc gtgcttgaaa tccgtgaagt cgaggttacc gacgaggccg
     2521 acgatctcgc gcgtgcggtg atcgacgaca acgacagcgc cgttatagat gtgcttgcgc
     2581 tggagctcgg gcgcgtgcag cgcgacaaca cgctcggcga gtgcctgcgc gccgggctcg
     2641 agcgtcgagc ggatgcgcac ctgccccggt gacagatcac gcagccagac cgcggcgtgc
     2701 gctgcgttgc gcggcatctg ccgcatcttc gtcggcgggg cgacagcggc tgcctcggcg
     2761 atcgcggctg cggtgtcatc cgcggagaac acgccggcgt cgatgagctt cttcaaaatc
     2821 gtgtcgcgac gctcgcgcag gcgctcggcg ttctgtttcg tcggcgagta gcgtgcgggc
     2881 ccttgcggca ccgcgagcag cgtcgcgatc tcgagtggcg agaggtgctg cgcgccatgg
     2941 ccgaagtaag accatgcggc cgactcgatg ccctcgaggt tgcggccgaa tggcgtacgc
     3001 gacaggtagt tctcgaggat ctcgcgcttc gacagccgcg catcgagttg catcgcgcga
     3061 aacatctcga tcagcttgct cgcgatcgtg cgcggccgcg gttcgagcaa gcgcgccagc
     3121 tgcatcgaga tcgtggagcc gccggagacg cgacggcgcg cgatgatgtc ggtccaggcc
     3181 gcgcgcgtga tcgcgatcgg atcgacaccg acgtgcgagt agaaccgttt gtcctcgagc
     3241 gcgatcaggg cagcggtgta cttcgggtcg acgcgatcga gcgtgaccgg caagcgccac
     3301 ttctcgtcgt gcgtgaggaa gatgtacgcc ggctgaccgt cgcgatactc gaccgcgacg
     3361 gaccaatcgc tgctgcgctc cggcagcggc accgcgaaca cggcgccgat cagtacgagc
     3421 catagcgcgt tgagcgcgat cgccgcgatc aacccgcgcc gcagccagcg ggcttttaga
     3481 gcagctttcc ggtccacggg ccgccgatca cggctttgcc gccatcctcg cgcgcccaca
     3541 gcgtcgggtc atacatcgcc tcgacctcga cgggcgggac cgtataggta cccgacgtca
     3601 ccgcgcgcac ggtgtacacg accttcttcg acgcgccgga ctgcagcata ccgaacgctt
     3661 cgaggtgatc gtcgcgcatg ttgaggaagt cgagctgcca ctgatcgtcg ggctcgatcc
     3721 agtcggcctt gaagctgcgg ccgagccgcg ggttctcgat ctcgaagccg gcgggtagcc
     3781 gatcgacgag cgcgaggttc tggatctggt tgccgctcgt gttcgccacc tcgacctcga
     3841 cgtacacgac ctcgccgagc ttgacctcgc ctttgctcag atcgatcgga tcgcccgcac
     3901 tgttgcgata cgtgcggctg accgtgagcc cgttgccgcc aaccttgtac ggctggttcg
     3961 ggcgcacgcc ttcgctcgag atcacgagcc acatgccgtc ggccgcttgc ggcaggtcga
     4021 gcgtcagcga cttgtactcg cttgcgcgct gcagtgacca tgacttgtcg ttcgtcggct
     4081 tcttcatctt gcgcggctcg atcgtggcgc catcggcgac gagcgtgccc gcggccgtgc
     4141 ccttgccgac ccggccctgc acccacttgc caagcccggt gatgccccag accagctcct
     4201 gggtattgta gtagtacgcg tacttctccg ccgtcaggat ctcggcgacg cggttcgcga
     4261 gcggctcgcc cgccggatcg ttgccgaaca gatcgaagaa cgtcgacagc tgcaggccgc
     4321 gacggcggcg atccgaatag aagctccagt agttcacgcg gtccttggtg accggcgacg
     4381 cgtcgacatt cttgaggtcc gccgcatagc gccgatcgcc agcgaggtag agcgccgcct
     4441 tcagcatgta gagatcctcg agttgctcgc cggtcgcttt cgccgccggg atttgctcga
     4501 tgagcttcag gatgcgcgcc ttcttgccct taccggcgag cgcgagcacg tagtggaggt
     4561 acgcctcggc ttgctcgtcg tagtggtgcc agctcgccgt gttcaggtga ccgctctcgc
     4621 gctcggcggc gcggttctcg atccacgtca gcactgcttg caggcgatcc tcggggaccg
     4681 cgtagccggc cttcttcgcg tcgagcagca tgtgcgtcgc gtacgcggtg ccccactcga
     4741 cgggctcggt cgaaccgggc cagtagccaa agccgcccga cggcgtctcc atcgacagca
     4801 cgcggttgat gccagacagc accatgtcct cgatcttcag ctgcgcgagt tgcggatcga
     4861 cctgctcgac gatgttgccg acgtagagca gaggccgcgt cgacgacgtc gtctgctcga
     4921 tgcagccgta gggatagtgg atgaggtact tcaggtggtc gaacgattcg ccgtacgggt
     4981 tcgaggtcaa ccagaacgtc gtctgctccg acagcggcat ccagcccttc agcgccggct
     5041 gcgacgcgag gtcgagcttg ccgggcgcga tcttgatctt ctgcacgaca cgctctttcg
     5101 ggcccgacgg caggaacgga atgtcgacct cgtcggcgac ctcgaacgcg cccgccttgc
     5161 ccttcgcctt cgcgacgaca cgcagcttcg cgccgccgac cggcatgttc gcgttcgcgc
     5221 ggaacacgag cgtctcggcg aggccatcct gaatcttcaa cgcgccattg tccttgccgg
     5281 caaacgtcag cggtgccgcc gcgttcttcg gttgcgcgag gccggcgatc ggaatcgccg
     5341 tgctgttcat cgtgacctgc acgtcgagcg ggccaccgga catgtttgtc atgaacaccg
     5401 gaatctgcat ctcgtcgccc tgcgtgacga agcgcgggaa cgtgacttga atgacgagcg
     5461 gatccttgac ggtgaccttc gcctcggcgc ggccgatctt cgtcggaccg gcggtgaccg
     5521 ccatgatgcg gagctcgccg cgataggtcg gcacgtggaa cgggatcgag accttgccgt
     5581 cgggaccgac cggcaacacg cccgagaaca gcgcgaccgg tttcaccggc tgcacgcggc
     5641 ccgcgtcgag cgcgccaccg ccgccttcgc tgccatcctc gtcgtagtca ccaccgcctg
     5701 tcttcgacga cgcgccggcc ggttgatgca gcatcgtcca gccgatcgtc tcgtacgtct
     5761 cgacgccgag cgcgcgcttc gcgaacagct cggtcagcgg atcgggtgtc ttgaagcccg
     5821 tcaacgacag gatgccctcg tcgacgacgg ccaccatggc gaacgt
//
LOCUS       DASUFX010000119         4772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1130663, whole genome shotgun sequence.
ACCESSION   DASUFX010000119 DASUFX010000000
VERSION     DASUFX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130663"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFV99_04570"
     CDS             <1..287
                     /locus_tag="VFV99_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058608.1"
                     /translation="HTQWSFTPARFDHSKVGCNLTGLHRTFACQDCHRTGNYVGLGAQ
                     CVNCHRDDSVRKDDLHKGYTTCANCHNPNYWTPQSPGTPAAAFGRESVCR"
     gene            284..2104
                     /locus_tag="VFV99_04575"
     CDS             284..2104
                     /locus_tag="VFV99_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058609.1"
                     /translation="MRRALAFALVLSAIAPPVWAKQEDEDEESDDSGDDEGSDEGDEE
                     AAAPKEEAKGATKEEEDAEDVTDDALRPKQDLSGHDLGTNKRSNEFERDRFFVDKVDT
                     EKTEDATLIQGSIASSTFLYKETGGAYQAPAGGMLMAGNNAGPTRLFTEMRLQTDFRH
                     IKASRWDARIDARARLVPQPTNTDPLTPTNQVQSGLTGKNEYELRELWLVRSGKRSDV
                     FVGRQFVADLGAVKFDGLRVDYAKSAKLTFIAFGGLYPVRGSRSISTDYVQLRDPESG
                     NNAGRFVTTGGFGGAYRTINAYGAIGGVAMYPLQKESPRFYVTSQGYLRSGSKLDFYH
                     FALVDLLGSNATEAGSHIQLTNLSAGVNVKPNPRLRLTASFNRVDTETLNVQAAAFLD
                     DLDTSATGNKIVQNDAQIIRLATDAARAGVSAGLGKNQRFEISTALSIRRRPELTLRS
                     PDNTVNATVPAAQSVEVWASIVDRHSIKQTRIGLDGGQTFGIGDVAYQRSEVFMGRLF
                     VNREIANGRGEWEGEANYSKVKDSTIGQNLTCTKLEDCFGTSNSTVMSVGGQLFYRLK
                     ADWFGIGTLHLMRIGNKRSDGLVDPPVTGITGFVRIAKRF"
     gene            2104..3216
                     /locus_tag="VFV99_04580"
     CDS             2104..3216
                     /locus_tag="VFV99_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058610.1"
                     /translation="MSPRGTLVVALVVAACSSSGKRVYEPQHVPRVPLRPGYLPFVDE
                     APMKRRPSIGLAAIAPAADRNVPWPLVELPSLQPHHDYAMARDLCTGAWAQRNKANAD
                     LVAYGAAWCRIRAGDRDGVVPLAQLARLAPSDIQRAARQDVINLVAGTDALGAFLRLR
                     ELKLDTVENLDTLAATYTALDMPEEAEVVVERVARLAKTATPQERCERLLAWSTLDDS
                     TLARLRELPDDLTGLCAKRAAATACAIDAAAQLLPNLAVVRECYNEFPEDADAERRAW
                     LLLAWVQWPVHTMPFWMALSRDAEKAIGVAGAEDLAVTALENAVRESACEPAELYDIA
                     GAAYRIANLPLHRAHYESRLDELRSMTEKRCSELHE"
     gene            3240..4022
                     /locus_tag="VFV99_04585"
     CDS             3240..4022
                     /locus_tag="VFV99_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5058611.1"
                     /translation="MSVGTVSLERRGHVLLMGLDRAAKRNAFDLPMWDALCAAYAELE
                     RDDELRVGVLHAKGDHFTGGLDLPQWGAAFSSGKWHIKDGTIDPLGLTGKRISKPVIA
                     AVQGTCLTIGIELMLATDIRIAAASTKFGQIEIKRGIYPVGGATIRFPREVGWANAMR
                     WLLTGDEFDAAEALRIGFVQEVVPHGEQLARAIALAEVVAAQAPLGVYATLASSRATI
                     SAQEIEAGNRLMPDLQPIMKTEDVQEGLRAFMERRPGSFKGR"
     gene            4059..4382
                     /locus_tag="VFV99_04590"
     CDS             4059..4382
                     /locus_tag="VFV99_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058612.1"
                     /translation="MKCSRWMMTALFALPMLTTTAFADEDRDDNHQHEMVKMEDLPAP
                     VKTTVQREAKGKTIVSIEKETENGATMYELELESKGKRTEVHISRTGKVLGRHPENHD
                     MPDYD"
     gene            complement(4383..>4772)
                     /locus_tag="VFV99_04595"
     CDS             complement(4383..>4772)
                     /locus_tag="VFV99_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058613.1"
                     /translation="VTQTLPMAQTAAGRYTADFKIQKYGSYLLKAVHKRNGNTVAESL
                     GSVALSYPLEYLRSSPDPEPLRHAAQVSGGHDQAKPAEVWDAQGEKVDYTEDLWPYVL
                     LVVVGLFILDLYAKRVRLFGYRTIRFQ"
BASE COUNT          882 a         1534 c         1605 g          751 t
ORIGIN      
        1 gccatacgca gtggtcgttc acgccggcgc gcttcgatca cagcaaggtc ggttgcaacc
       61 tgacgggttt gcaccgcaca tttgcgtgtc aggactgcca ccgcaccggt aactacgtcg
      121 gcctcggcgc gcagtgcgtg aactgccacc gcgacgactc cgtgcgcaaa gatgacctgc
      181 acaagggcta cacgacctgc gcgaactgcc ataacccgaa ctactggacg ccgcagtcgc
      241 cggggacacc tgccgcggcg tttggccgag agtcggtgtg ccgatgaggc gcgcgctcgc
      301 gttcgcgctc gtgctctctg caatcgcacc gccggtgtgg gcgaagcaag aggacgagga
      361 cgaagagtcc gacgacagcg gcgatgacga aggcagcgac gagggcgacg aggaagcggc
      421 cgcgcccaaa gaagaggcga agggcgcgac gaaggaagaa gaggacgccg aggacgttac
      481 ggacgacgcg ctgcggccga agcaggacct gagcggtcac gatctcggca cgaacaagcg
      541 gtcgaacgag ttcgagcgcg atcggttctt cgtcgacaag gtcgataccg agaagaccga
      601 ggacgccacg ctgatccagg gcagcatcgc gtcgagcacg ttcctctaca aggagacggg
      661 cggggcctac caggcaccag ccggcggcat gctgatggct ggcaataacg ccggtccgac
      721 ccggctgttc accgagatgc ggctgcagac cgacttccgc cacatcaagg cgtcgcgctg
      781 ggacgctcgc atcgacgcgc gcgcgcgcct cgtgccgcaa ccgacgaaca cggatccact
      841 cacgccaacg aaccaggtcc agtccgggct caccggcaag aacgagtacg agctacgcga
      901 gctctggctc gtgcgctcgg gcaagcgcag cgacgtgttc gtcggtcgcc agttcgtggc
      961 cgatctcggc gcagtcaagt tcgacggcct gcgtgtcgat tacgcgaagt cggcgaagct
     1021 gacgttcatc gcgtttggtg gtctctaccc ggtacgcggc tcgcgctcga tctcgacgga
     1081 ctacgtccag ctgagagatc cagagtccgg aaacaacgcc ggtcgattcg taacgaccgg
     1141 cgggtttggt ggcgcgtacc gcacgatcaa cgcgtacggc gcgatcggcg gcgtcgcgat
     1201 gtacccgctg cagaaggagt cgccgcggtt ctacgtcact tcacagggct acctgcgcag
     1261 cggcagcaag ctcgacttct atcacttcgc gctcgtcgac ctcctcggca gcaacgcgac
     1321 cgaagcaggc tcgcacatcc agctcacgaa cctgtcggca ggcgtcaacg tcaagccgaa
     1381 cccgcgcctg cgcctgaccg cgagcttcaa tcgcgtcgat accgagacac tcaacgtgca
     1441 ggccgccgcg ttcctcgacg acctcgacac gtcggcgacc ggcaacaaga tcgtccagaa
     1501 cgatgcccag atcatccggc tcgcgaccga cgccgcccgt gccggcgtgt cggcgggtct
     1561 tggcaagaac cagcgcttcg agatctcgac ggcgctgtcg attcgtcgcc gccccgagct
     1621 cacgctgcga tcgccggaca atacggtgaa tgcaacggtg ccggcggcgc agagcgtcga
     1681 ggtttgggcc agcatcgtcg atcgccactc gatcaagcag acgcggatcg gcctcgatgg
     1741 cggccagacg tttggcatcg gtgacgtcgc gtaccagcgc agcgaagtgt tcatgggccg
     1801 cctgttcgtc aaccgcgaga tcgcgaacgg tcgcggcgag tgggagggcg aggcgaacta
     1861 ctcgaaggtc aaggacagca cgatcgggca gaacctgacc tgcacgaagc tggaggactg
     1921 cttcggcacg tcgaacagca ccgtgatgtc ggtaggcggc cagctgttct accgcctgaa
     1981 agctgactgg tttggcatcg gtacgctgca cctcatgcgg atcggcaaca agcgcagtga
     2041 tgggcttgtc gatcctcccg tgactggcat taccggcttt gtgcggatcg ccaagcgctt
     2101 ctgatgtccc cgcgagggac gctggtcgtc gcgctcgtcg tcgcggcgtg ttcgtcgtcg
     2161 ggcaagcgcg tctacgagcc gcagcacgtg ccgcgcgtgc cactgcggcc tgggtacctc
     2221 ccgttcgtcg atgaagcgcc gatgaaacgg cggccgagca tcgggctcgc ggcgatcgcg
     2281 ccagccgccg accgcaacgt accgtggccg ctcgtcgagc tgccgtcgct ccagccgcat
     2341 catgattatg caatggcgcg cgacctgtgt actggcgcgt gggcgcagcg caacaaggcg
     2401 aacgcggacc ttgtcgcgta cggcgctgcg tggtgccgca ttcgcgcagg ggaccgtgat
     2461 ggggtcgtgc cgctcgcgca gctcgcgcgg ttggcgccga gcgacatcca gcgggccgcg
     2521 cgccaggacg tcatcaacct cgtcgccggc actgacgctc tgggggcgtt cttgcgactg
     2581 cgcgagctga agctcgacac cgtcgagaac ctcgacactc tcgccgcgac gtacaccgcg
     2641 ctcgacatgc cggaggaagc ggaggtcgtc gtcgagcgcg tcgcgcggct cgccaagacc
     2701 gccacaccgc aagaacgctg cgagcggttg ctcgcgtggt ccacgctcga cgacagcaca
     2761 ctcgctcggc tgcgcgagct tcccgacgac ctcacgggcc tgtgcgcgaa gcgggctgcc
     2821 gcaactgcat gtgcgatcga cgccgccgcg cagctcctgc ccaatctcgc ggtcgtgcgc
     2881 gagtgctaca acgagtttcc cgaggacgcg gacgccgaac ggcgcgcatg gctgctgctc
     2941 gcctgggtcc agtggcccgt gcatacgatg ccgttctgga tggcactgtc gcgtgacgcc
     3001 gagaaagcga tcggcgtggc gggggcggag gatctcgcgg tcaccgcgct cgagaacgcg
     3061 gtgcgcgaat ctgcgtgcga gccggccgag ctctacgaca tcgcgggcgc cgcgtaccgg
     3121 attgcgaatc tgccgctcca tcgcgctcac tacgaatcgc gcctcgatga gctgcgctcg
     3181 atgaccgaga agcgctgcag cgagctgcac gaatagcaga gccgacatac agttcgcgca
     3241 tgtcggttgg aaccgtttcg ctcgaacgtc gcggtcacgt attgctgatg ggtctcgatc
     3301 gcgctgccaa gcgcaacgcg ttcgatttgc cgatgtggga cgcgctatgc gcagcctacg
     3361 ccgagctcga acgcgacgac gagctgcgcg tcggcgtgct gcacgcgaag ggcgaccact
     3421 tcacgggcgg cctcgacctg ccgcagtggg gcgcggcgtt ctcgtcgggc aagtggcaca
     3481 tcaaggacgg cacgatcgat ccgctcgggc tcacgggtaa gcgcatcagc aagccggtga
     3541 tcgcggcggt gcagggcacg tgcctcacga tcggcatcga gctcatgctc gcgaccgaca
     3601 ttcggatcgc tgcggcgtcg acgaagttcg gccagatcga gatcaagcgc ggcatctatc
     3661 cggtcggcgg cgcgacgatc cggtttccgc gcgaggtcgg ctgggcgaat gcgatgcgct
     3721 ggctgctcac cggcgacgag ttcgacgcgg cggaagcatt gcgcatcggc ttcgtgcagg
     3781 aagtcgtgcc gcacggcgaa cagctcgccc gcgccatcgc gctcgccgag gtcgtcgctg
     3841 cgcaggcgcc gctcggcgtg tacgcgacgc tggcctcgtc acgcgccacg atctcggccc
     3901 aggagatcga ggccggcaat cggctgatgc ccgacctcca gccgatcatg aagaccgagg
     3961 acgtgcagga aggcctacgt gcgttcatgg agcgccggcc gggcagcttc aagggccgtt
     4021 aggcacacac actgcttgtg agcacttcag aggaacccat gaagtgctct agatggatga
     4081 tgactgcgtt gttcgcgcta ccgatgctta cgacgacggc gtttgccgac gaggacaggg
     4141 atgacaacca tcaacacgag atggtgaaga tggaggattt gcctgctccg gtgaagacca
     4201 cggtacagcg cgaggctaag ggtaagacca tcgtctcgat cgagaaagag accgagaacg
     4261 gcgcgacgat gtacgagctc gagctcgaat cgaagggtaa gcgcaccgag gtccacatct
     4321 cgcgaacggg caaggtgctc gggcgtcatc ccgaaaatca cgacatgccc gactacgact
     4381 aattactgga agcgaatcgt gcggtagccg aacaggcgca cgcgtttcgc atacaggtcg
     4441 aggatgaaca ggccgacgac gacgagcagc acgtacggcc acaggtcctc cgtgtaatcg
     4501 accttctcgc cttgcgcatc ccacacctcg gcgggcttcg cctgatcgtg gccgccggag
     4561 acctgcgctg cgtggcgcag cggctcggga tccggcgacg agcgcagata ctcgagcgga
     4621 taggagaggg cgaccgagcc gagcgactcg gcgacggtgt tgccgttgcg cttgtgcacg
     4681 gccttgagca gatagctgcc gtacttctgg atcttgaagt cggcggtgta acggccggcg
     4741 gcggtttgcg ccatcggcag cgtctgcgtg ac
//
LOCUS       DASUFX010000120         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_297173,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000120 DASUFX010000000
VERSION     DASUFX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297173"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..959
                     /locus_tag="VFV99_04600"
     CDS             114..959
                     /locus_tag="VFV99_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058614.1"
                     /translation="MAQLDPARQATLAAVANLASVVDAHYAAGTTAWPSVTVSRERYI
                     EELARRITMRGSEAADRVLLTMPGADLYLVVACADNDATALAAFRDAFVPGLRQALGR
                     LALEPPTIDETIQRVLVMLFVDGQIKGYGGKGTLRSWVRSIAVRTGRRLAGVGHDSGS
                     DDDLAELPAAVADPELEMLRARYAEQVRVAFAAAFAELSERERNVLRQYHMDGLTIDQ
                     LAALYQVNRATTARWVAGARLAIVARTRNQLVDRFGIAATEVDSIIRLVRSQLAVSVR
                     DLATE"
     gene            1006..2055
                     /locus_tag="VFV99_04605"
     CDS             1006..2055
                     /locus_tag="VFV99_04605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058615.1"
                     /translation="MHAIPTPDPDTANDVFDGGTLYCPICGESFSPLCDRCPDDECPV
                     VGRPLTGDPFIGRLLDRRFRVVQLLAQGAMGTVYEGVQLPIKRPVAIKVIRDELGNDD
                     RTAQRFLREAHLLTRIAHPNIVDVLDFGETPDGHLYLVMDRLRGKTLDVALAEIGRFS
                     VRRTCEIGLQLCNALVAAHAHGVVHRDLKPANIYLLAELGDWVKVLDFGLAKTITADP
                     ASEITVTGAVLGTPLYMAPETITDNTADPRSDLYALGCILHEMLNGAPAFYGESTALV
                     LARHLDDPPPALPIMVPPTLRQLIYALLAKEPDQRPANALTVCAVLEHCLAAELVEDM
                     PTLIQPALPDLDEKS"
     gene            2147..>3702
                     /locus_tag="VFV99_04610"
     CDS             2147..>3702
                     /locus_tag="VFV99_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5058616.1"
                     /translation="MARSLGPGRNDPKREPDAIIVEGAHEHNLQVDRLELPKHRLVVI
                     TGPSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLGQMEKPKYERIRGLSPTIAIQQ
                     KSASSNPRSTVGTITEIYDYLRVLYARAGEQRCYQCGGPVGARTASEIVDELAGLPAK
                     TAVTVLAPKAENRKGEFRELFAELRKAGFVRVRIDGMVVRLDDVDALEKQKKHSIELV
                     IDRITINPDDKGRLTDSVETALREGKGKIMVDVPGEKIPRVYSEANACPTCGIGFPEL
                     SPQSFSFNSPLGMCVECNGLGERLAADPDLVVPDPTRSIRDGAVAVWGEAIAKDSGWT
                     TNIVKALAKAFKIDLDKPWNKLGEKQKDVLLYGTGDKRVNVTWEGRHSTGEWAMRFEG
                     ILAQLERRHRESSSDRTKAHYEQFFRAIPCAVCNGTRLRPESRAVFVNEKPIVEVTGM
                     TVKQTYQFISSMKLTGSRAQIAGEVLKEIKNRLTFLLDVGLDYLTLHRSAGTLSGGEA
                     QRIRLASQLG"
BASE COUNT          634 a         1271 c         1201 g          596 t
ORIGIN      
        1 cgagccggcc gtcgacgtag gccgcgagcg tgtcggggtc gaggtgctgg tccatgagcg
       61 cgctcgtcgc catgctagcc tgggtggcat cgcctgtgac tgtcgcgatg ctcatggcac
      121 agctggaccc cgcccgccaa gcaactcttg cagcggtcgc aaatcttgcc agcgtcgtcg
      181 acgcccacta cgccgccggg accaccgcat ggcccagcgt cacggtcagc cgcgaacgct
      241 acatcgagga gctcgcgcgc cggatcacga tgcgcggcag cgaggcagcc gaccgcgtgc
      301 tgctcacgat gccaggcgcc gacctgtacc tcgtcgtcgc gtgcgccgac aacgatgcca
      361 ccgcgctcgc ggcatttcgc gacgcgttcg tgccgggatt gcgccaggca ctcggcaggc
      421 tcgcgctcga gccgccgacg atcgacgaga cgatccagcg cgtgcttgtc atgctgttcg
      481 tcgacggcca gatcaaaggc tacggtggta agggcacgct gcgcagctgg gtgcgctcga
      541 tcgcggtccg cacgggccgg cggctcgccg gcgtcggcca cgattccggc agtgacgacg
      601 atctcgccga gctacccgcc gcagtcgccg accccgagct cgagatgctg cgcgcacgtt
      661 atgcggagca agtccgggtc gcgttcgcgg ccgcgttcgc cgagctgtcc gagcgcgagc
      721 gcaacgtgct gcgccagtac cacatggatg gcctcaccat cgatcagctg gctgctctct
      781 atcaggtcaa ccgggccacg accgcccgct gggtggccgg cgcgcggctc gcgatcgtcg
      841 cccggacccg aaatcagctc gtcgaccgat tcgggatcgc agcgacggaa gtcgacagca
      901 tcattagact cgtgcgcagt cagctcgcgg tcagcgtccg cgatctcgcg actgaatagc
      961 gctcgcaatg cgacaaatcg ctgccacctc ctaccagggg gtgccatgca cgcgatccca
     1021 accccggatc ccgataccgc caacgacgtc ttcgacggcg gaacgctgta ctgcccgatc
     1081 tgcggcgaat cgttctcgcc gctgtgtgac cggtgtccgg acgatgagtg tccggtcgtc
     1141 ggccgtccgc ttaccggcga tccgttcatc ggccgactgc tcgatcgccg gtttcgcgtc
     1201 gtccagctgc tcgcacaggg cgcgatgggc acggtctacg aaggtgtgca gctgccgatc
     1261 aaacggccgg tcgcgatcaa agtgatccgc gacgagctcg gcaacgacga cagaaccgcg
     1321 cagcgcttct tgcgcgaggc gcatctgctc acgcgcatcg cgcacccgaa catcgtcgac
     1381 gtgctcgact tcggcgagac gccggacggc catctctacc tcgtgatgga tcggttgcgc
     1441 ggcaagacgc tcgacgtcgc gctcgcggag atcggtcgat tctcggtgcg ccgcacgtgc
     1501 gagatcgggc tgcagctgtg caatgcgctc gtcgctgcgc atgcgcacgg tgtcgtgcat
     1561 cgcgatctca agccggcgaa catctacctg ctcgccgagc tcggcgactg ggtgaaggtc
     1621 ctcgacttcg gactcgccaa gacgatcacg gccgatccgg cgagcgagat cacggtcacc
     1681 ggcgccgtcc tcggcacgcc gctctacatg gcgcccgaaa cgatcacgga caacacggcc
     1741 gatccgcgca gcgatctcta cgcgctcggc tgcatcctgc acgagatgtt gaacggcgcg
     1801 cccgcgttct acggcgagtc gaccgcgctc gtgctcgccc gccacctcga cgatccgccg
     1861 cccgcgttgc ccatcatggt cccgcccacg ctgcgccagc tcatctacgc gctgctcgcg
     1921 aaggaacccg atcagcgccc ggcgaatgcg ctgacggtat gcgcggtcct cgagcactgc
     1981 ctcgccgcgg agctcgtcga ggacatgccg accctcatcc agccggcgct cccggacctc
     2041 gacgaaaaat cctgacagcc gtttgtcacg gtttcgtcga tctgacttcg gcccgccgtt
     2101 tcgccagctt gacacctgaa cagacgctcc gtagggtgtc gttcctatgg ctcgtagcct
     2161 cggtcctggt cgtaacgatc ccaagcgcga gcctgacgcg atcattgtcg agggggccca
     2221 cgagcacaac ttgcaggtcg accggctcga gctgcccaag caccggctgg tcgtgatcac
     2281 cgggccgtcg ggctcgggaa agtcgtcgct cgcgttcgac acgctctacg ccgagggcca
     2341 gcgccgctat gtcgagtcgc tgtcggcgta cgcgcgccag ttcctcggcc agatggagaa
     2401 gccgaagtac gagcgcatcc gcgggctgtc accgacgatc gcgatccagc agaagagcgc
     2461 gtcgagtaac ccacgctcga ccgtcggcac gatcaccgag atctacgact acctgcgcgt
     2521 gctctacgcg cgtgccggcg agcagcgctg ctatcagtgc ggcggtccgg tcggcgcgcg
     2581 caccgcgagc gagatcgtcg acgagctggc tgggctgccc gcgaagacgg cggtcacggt
     2641 gctcgcgccg aaggccgaga accgcaaggg cgagttccgc gagctgttcg ccgagctgcg
     2701 caaggccggc ttcgtgcgcg tgcgcatcga cggcatggtc gtgcgactcg acgatgtcga
     2761 cgcgctcgaa aaacagaaga agcactcgat cgagctcgtg atcgatcgca tcacgatcaa
     2821 tcccgatgac aagggccgcc tcaccgactc ggtcgagacc gcgctgcgcg agggcaaagg
     2881 caagatcatg gtcgacgtgc cgggtgaaaa gatcccgcgc gtctattcgg aagcgaacgc
     2941 gtgcccgacg tgcggcatcg gcttccctga gctgtcgccg cagagcttct cgttcaactc
     3001 gccgctcggc atgtgcgtcg agtgcaacgg cctcggcgag cgtctcgctg ccgaccccga
     3061 cctcgtcgtg ccggatccga cgcgctcgat ccgcgatggc gcggtcgcgg tgtggggcga
     3121 ggccatcgcg aaagattccg gctggacgac aaacatcgtc aaggcactcg cgaaggcatt
     3181 caagatcgat ctcgacaagc cgtggaacaa gctcggcgag aagcagaagg acgtgctgct
     3241 ctacggcacg ggcgacaaac gcgtcaacgt gacgtgggaa ggccgccatt cgaccggcga
     3301 atgggcgatg cgcttcgagg gcatcctcgc gcaactcgag cgccggcacc gcgagtcatc
     3361 gagtgatcgc acgaaggcgc actacgagca gttcttccgc gcgatcccgt gcgcggtctg
     3421 caacggcacg cgcttgcggc cggagtcgcg cgccgtgttc gtcaacgaga aaccgatcgt
     3481 cgaggtcacc ggcatgacgg tgaagcagac gtaccagttc atctcgtcga tgaagctcac
     3541 cggttcgcgc gcgcagatcg caggcgaggt gctcaaggag atcaagaacc ggctcacgtt
     3601 cttgctcgac gtcggcctcg attacctgac gctgcaccgc tcggcgggca cgctgtcggg
     3661 tggcgaggcg cagcgcattc ggctcgcatc acagctcggc ag
//
LOCUS       DASUFX010000121         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1130977, whole genome shotgun sequence.
ACCESSION   DASUFX010000121 DASUFX010000000
VERSION     DASUFX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130977"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFV99_04615"
     CDS             complement(<1..315)
                     /locus_tag="VFV99_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058617.1"
                     /translation="MNESSAVSDIIQRMNSRRLQTDPADALLFAPATGGAPRARRDQA
                     RWFESSAVETAPVRAPQPVAASPRANPRAQEPAARTAPERGRVGLLIVATFVAGAVGI
                     YGA"
     gene            complement(468..1874)
                     /locus_tag="VFV99_04620"
     CDS             complement(468..1874)
                     /locus_tag="VFV99_04620"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEX5058618.1"
                     /translation="MTSSLAKTWPVLACLASGCSHGPSSDGVDAAVVVEPALVETTFT
                     EDPANVLAPERGFYTGIDLLGGSDFASVRAGGMTLAIASIHLDSYRTTALDPNLLDGL
                     SAGFMRVRAAGIKVILRFSYNDGPNGAPDASLAQIEAHIAQLAPILRANADVIAFMQA
                     GFIGAWGEWHDSTNGLDTTPNRLAIISALLDALPANRMVQVRTPGFVDAMFPGGPLDP
                     AQGFSGTPRSRVGHHNDCFLASPTDYGTYADPVETWKDYIAADSRFTPMGGETCNVDA
                     PRSDCESATAEMARLHMTYVNSQYHQGVLDGWTTQGCMPAIQKRLGYRLVLESARHSE
                     QVKPGGRLRLEIALRNDGFASPLNVRPLRVVLDRGSERYVVTLANVDVRRWEAGEHQV
                     ATTLRIPSELAAGTYRVSLWLPDADLETRPEYAIAFANTDVFDATAGLDVIVPALVVD
                     PNAPGEAIGDAHELVELP"
     gene            1988..2599
                     /locus_tag="VFV99_04625"
     CDS             1988..2599
                     /locus_tag="VFV99_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5058619.1"
                     /translation="MTSPKINLSVYHDGHRQLQDQFDSRRIADRLAQHTYRNALTDGD
                     RNFIEGASMFFLATADPDGNPDCSYKGGDPGFVRVVSANEIAWPDYDGNGQFRSLGNV
                     AVHSPVALLFIDWTRPNRLRLHGHARLCFEDPLLADMPGGQLVVRVSIERVFPNCPRY
                     IHTMQEVARSPYVPHEGEDAPVPGWKKNELFADALPVRAKADE"
     gene            2592..3773
                     /locus_tag="VFV99_04630"
     CDS             2592..3773
                     /locus_tag="VFV99_04630"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5058620.1"
                     /translation="MSDSAATLSAWLLDPTTSELEPSTLLDGLAVRLAAADLDLHRIS
                     IWIPTKHPELWGHQLMWTPDGGATVTRRSHEVSESTDYVGTPAEALHNSGNSTLRCRL
                     DRAPDRNRFAMLAKMADAGATDYLLTSAEPRRRHGSWVSYATRRSGGFDDAQLALLQQ
                     LTPVLALHIQLLGAHFATRSLLEVYLGRNAARRVIDGEFRRGTGTEIDAALWFCDMRG
                     FTAMSDRLPARAVVGVLDRYFEHVATPIEEHGGEILKFIGDATLAVFPIGEASAADAC
                     RRALAAAQAVLTRIDAWSSSEAGAIQLGVALHTGRVFYGNIGGRERLDFTVIGASVNE
                     VCRVEAMCKTLGEPLLMTSAFVAALGDSGHSGDVVSLGNHALKGVAQSQEILTMRRRP
                     M"
     gene            3784..>4267
                     /locus_tag="VFV99_04635"
     CDS             3784..>4267
                     /locus_tag="VFV99_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058621.1"
                     /translation="MSASALHGRDAALAVLRGALDAALGGRGGLVVVSGEAGIGKSAL
                     ATELARESEQRGALVTWGRAWEFADAPPYFPVLPCLRALGLEAHGDAFALWEQVLGAL
                     AHAPTPLVWLVEDVHAADLGTLDLLTFLAQPLRAVRALVVATARLADARVDERMLQRL
                     A"
BASE COUNT          694 a         1441 c         1416 g          716 t
ORIGIN      
        1 cgcgccatag ataccgaccg cacctgcgac gaacgtcgcc acgatcagca gtcctacgcg
       61 gccgcgttcc ggcgccgtcc gcgcggccgg ctcttgcgca cgtggattcg cacgtgggct
      121 tgccgcgact ggctgtggtg cgcgaaccgg ggcggtctcg actgccgagc tctcgaacca
      181 gcgcgcctga tcgcgacgcg cacgtggcgc gcccccggtc gcgggcgcga acaacagcgc
      241 atcggccggg tcggtctgga gtcgtcgcga attcatgcgc tggatgatgt cgctgactgc
      301 gctcgattcg ttcatagagg cccaccgtcg agcctgcagt cagtagacca cttcgcatac
      361 atggattccg cgaacttatc gtcgagggga tgagagtcga gcacccgtca ccagctgccc
      421 caggggtcga gagtccctag atccggcgag gacggctcac gcgatggtca cggaagctcg
      481 acgagctcgt gcgcatcgcc gatcgcttcg ccgggtgcgt tcggatccac gacgagtgcg
      541 gggacgatga catccagccc ggccgtcgca tcgaacacgt cggtgttggc gaacgcgatc
      601 gcgtactcgg ggcgcgtctc gagatccgca tcgggcaacc acagcgacac ccgatacgtt
      661 ccggccgcga gctccgacgg aatacgcagt gtcgtcgcga cctggtgctc gcccgcttcc
      721 cagcgacgaa cgtcgacgtt cgcgagcgtc acgacgtatc gctcactccc acgatcgagc
      781 acgacgcgca gcggccgcac gttgagcggc gatgcaaaac catcgttacg cagcgcgatc
      841 tcgaggcgca gacgtccgcc cggtttgacc tgctcggagt gacgcgccga ctccagtacg
      901 agccgatagc cgagccgctt ctggatcgcc ggcatgcagc cttgcgtggt ccatccgtcg
      961 agcacgccct ggtgatattg cgagttgacg tacgtcatgt gcaggcgcgc catctcggcg
     1021 gtcgcgctct cgcaatcgga gcgcggtgca tcgacgttgc aggtttcacc gcccatcggt
     1081 gtgaagcgcg agtccgcggc gatgtagtcc ttccacgtct cgacggggtc ggcatacgtg
     1141 ccgtaatcgg tcgggctcgc gaggaagcaa tcgttgtgat ggccgacgcg tgaacgcggg
     1201 gtgccagaga agccctgcgc gggatcgagc ggaccgccgg ggaacatcgc gtcgacgaag
     1261 ccgggcgtcc gcacctgcac catgcgattc gcgggcaacg cgtcgagcag cgcgctgatg
     1321 atcgcgagcc ggttaggagt cgtgtcgaga ccgttcgtgg agtcgtgcca ctcgccccac
     1381 gcgccgatga acccggcctg catgaatgcg atgacgtccg cgttggcgcg gaggatcggt
     1441 gcgagctgtg cgatgtgagc ttcgatctgc gcgagcgacg catccggcgc accgttcgga
     1501 ccgtcgttgt agctgaaccg caggatcact ttgattccgg cggcgcgaac gcgcatgaac
     1561 cccgcgctga gtccgtcgag caagttcgga tcgagcgccg tcgtgcgata gctatcgaga
     1621 tggattgatg caatcgcgag cgtcatgcca ccagcgcgca cgctcgcgaa gtcggatccg
     1681 ccgagtagat cgatgccggt gtagaaaccg cgctcgggcg cgagcacgtt cgccggatcc
     1741 tctgtgaagg tggtctcgac gagcgcgggc tcgacgacca ctgcggcatc gactccatcg
     1801 gacgagggcc cgtgcgaaca gccgctcgcg aggcaggcga gaactggcca ggtcttcgcg
     1861 agcgagctcg tcatggacag cgatgtaacc cccgagcagg tgcgccggac aacgaaaaag
     1921 gatgaccggt tcttacagcg acgccctccg tatcgcccac gcttcgccga acgtgtaatg
     1981 gtgaagcgtg acctcaccca agataaacct gagcgtgtat cacgacggtc accgccagct
     2041 gcaggaccag ttcgactcgc gccgcatcgc cgatcgtctg gcacagcaca cgtaccgcaa
     2101 cgcgctcacc gacggcgatc gcaacttcat cgaaggtgcg agcatgttct ttctggcgac
     2161 cgcggacccc gatggcaatc ccgattgctc gtacaaaggc ggcgatccgg gcttcgtgcg
     2221 cgtcgtgtcg gcgaacgaga tcgcgtggcc cgactacgac ggcaacggcc agttccgctc
     2281 gctcggcaat gtggccgtgc attctccagt cgcgctcctg ttcatcgatt ggacgcgtcc
     2341 gaaccgcctg cgcctccacg gtcacgctcg gctgtgtttc gaagatccgc tgctcgccga
     2401 tatgccgggc ggccaactcg tcgtgcgggt cagcatcgag cgcgtgtttc ccaactgccc
     2461 gcggtacatt cacacgatgc aagaggtggc gaggtcgccg tatgtcccgc acgagggcga
     2521 agacgcgccc gtgccgggct ggaaaaagaa cgagctcttc gccgatgcgc ttcccgtgcg
     2581 cgccaaagcg gatgagtgat tccgccgcca cgctctcggc ctggctcctc gatccaacga
     2641 cgagcgagct cgagccgtcg acgcttctcg acggtctggc ggtgcgtctc gctgccgccg
     2701 acctcgatct ccaccgcatc agcatctgga tcccgaccaa gcatcccgag ctatggggtc
     2761 accagctcat gtggacgccg gatggcggag cgacagtcac gcgccgctcg cacgaggtct
     2821 cggaatccac cgactacgtc ggcacacccg ccgaggcgct ccacaatagc ggcaacagca
     2881 cgcttcgctg ccgcctcgat cgagcgcccg accggaaccg cttcgcgatg ctcgcaaaga
     2941 tggcggacgc cggcgccacc gactacctgt tgacgtcggc cgagccacga cggcgccacg
     3001 gctcgtgggt gagctacgcg actcgacgct cgggcggctt cgacgatgcc cagctcgcgc
     3061 tcctccagca gctaacgccg gttctcgcgc tgcacatcca gctgctcggc gcccatttcg
     3121 cgacccggtc gctactcgag gtctacctcg gccgcaacgc cgctcgccgc gtgatcgacg
     3181 gcgagttccg ccgcggcact ggcaccgaga tcgacgccgc tctctggttc tgtgacatgc
     3241 gtgggttcac cgcgatgagc gatcgattgc cggcacgcgc cgtcgtcggc gtgctcgatc
     3301 gttacttcga gcacgtggcc acaccgatcg aggagcatgg cggcgagatc ctgaagttca
     3361 tcggcgacgc gacgctcgcc gtcttcccga tcggcgaggc cagtgctgct gacgcgtgcc
     3421 ggcgcgcgct cgccgccgcg caggctgttc tgacccgcat cgatgcttgg tcctcgagcg
     3481 aggccggcgc gatccagctc ggcgttgccc ttcacacggg cagagtgttc tacgggaaca
     3541 tcggcggccg cgaacgcctc gacttcaccg tgatcggtgc gtcggtcaac gaggtctgcc
     3601 gcgtcgaggc gatgtgcaag acgcttggcg aaccgctgct gatgacgtcg gcgttcgtcg
     3661 cggcgctcgg cgacagcggc cacagcggag atgtagtctc gctcggcaat cacgcgctga
     3721 agggcgtcgc acagtcgcag gagatcctga cgatgcgccg tcgccccatg tagactggcg
     3781 ccggtgtccg cttctgctct gcatggtcga gatgcggcgc tcgcggtgtt gcgcggggcg
     3841 ctcgacgccg cgctcggcgg acgcggcggg ctcgttgtcg tgtcgggcga ggccggcatc
     3901 ggcaagagcg cgctcgcgac cgagcttgca cgcgagtcgg agcagcgagg cgcgctggtc
     3961 acgtgggggc gcgcgtggga gttcgccgac gcaccgccgt actttcccgt gctgccatgc
     4021 ctgcgcgctc tcgggctcga agcccacggc gacgcgttcg cgctgtggga gcaagtgctc
     4081 ggtgcgctcg cacacgcgcc gacgccactc gtgtggctcg tcgaagatgt gcatgctgcc
     4141 gacctcggca cgctcgatct gctgacgttc ctcgcgcagc cgctacgcgc ggtacgtgca
     4201 ctcgtggtcg cgaccgcgcg gctggccgat gcacgcgtcg acgagcgcat gttgcagcgg
     4261 ctcgcgc
//
LOCUS       DASUFX010000122         5499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_854331,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000122 DASUFX010000000
VERSION     DASUFX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854331"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFV99_04640"
     CDS             <1..321
                     /locus_tag="VFV99_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058622.1"
                     /translation="ERLRSFLRDVFAVFESHRKFIKVAVETEHFKQTPSTTQNDLASA
                     IGEIVKAGVKEKVLSPRLAEMLPFVISGTLKGYGLRRIGENGALEAADAEALVTIFLD
                     GARK"
     gene            318..1856
                     /locus_tag="VFV99_04645"
     CDS             318..1856
                     /locus_tag="VFV99_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5058623.1"
                     /translation="MSATPAPVNKWVVAVSIAFGSLMATIDTSIVNVAMPQIRGELGA
                     SLQEITLITTGYMIAMVLVMPLTGFLGALFGQKRVYLASLAIFTIGSVLCGTARSLEM
                     LVVWRILQGLGGGALQPTQQAILRQTFPPEEQGMAMALFSMVIMVGPAVGPVLGGWLT
                     DGFSWPWIFFVNLPVGLLGIYLTMRNVHEPEDVRVENRARAQIVKKHFDYAGIILMGV
                     AIGGLQYVLEEGSAADWFDSTKIVVATYIAGISLAAFIIRELTATAPVVDLRMFRDPT
                     FASGTAIAGLVFAMLMGSMFLLPVFTQEMLHYSATQSGIVLLPRTIAMMIASPFVGKL
                     YNRTRPAYLVAFGMLATALGCWMQADITLHTSTSDLFWPLAVTGIGFAFLFVPLTTAA
                     LSNVPRANLAGAAGVNSFVRQVGGSIGLSIFATMFQNYGARAQAALASGISVLRPEVA
                     AQVDAGLTAHAAYGRTALEGVVIGFDKMFLLQTFAFVIVLPLLLFLRVQRQAAPAHVE
                     LPAE"
     gene            1861..3045
                     /locus_tag="VFV99_04650"
     CDS             1861..3045
                     /locus_tag="VFV99_04650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5058624.1"
                     /translation="MEASVKPKPRRGKRAYLILGVISLIVAGLWLGHRLWTRGKQSTD
                     DAQVEADVTPIASRVAGTIAATKVHDNQAVKKGDVLFEIDPANLDVEVLRTEADLEAA
                     RAQLGAAQAQVAIVHSSSKGGLSSAQAALSGAAASARGAADSIRAADAAVARARADLQ
                     NTQSELARVQALFDKEAVTRQELEHAQHARDVAQAALNAATAQLDGARDQRNMAQARV
                     SEAEGKVAQSAPVDQQVAAAQAAADLATARVKAAEAAVAKAKLDRAHATIVAPVDGVV
                     SKLGAHVGQSVNIGQPLLMLVPNETYVVANFKESQIAHMKPGDKVDIELDAFEGDFAG
                     VVETLSPATGARFSLIQPDNATGNFVKVVQRVPVKIKWADTPKVSVRPGLSAEVTVHV
                     GD"
     gene            3070..3447
                     /locus_tag="VFV99_04655"
     CDS             3070..3447
                     /locus_tag="VFV99_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5058625.1"
                     /translation="MRYAALVLVMLVGTATADPAADETAAYENAKPVFTKYCASCHTK
                     AGAKATAKKLDHFDMTTYPFGGHHATTIAATMRKVLAIKGGKPTMPSDRKGAVKGDEL
                     ALIAAWCDAFDAAHKANSTHHHH"
     gene            3619..4863
                     /locus_tag="VFV99_04660"
     CDS             3619..4863
                     /locus_tag="VFV99_04660"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5058626.1"
                     /translation="MRTATILTALLVVVPSVAAAEDQITVDNQVRPDLFASPPTDTHI
                     TFVRKMLPATNAPIAAVAQSRIIYLNKNGVTLTPGNDDSRTNRSSIISSQRTVPAWNA
                     SATLWSDTVKCMTDMFSRWDVQVTDVNPGNVPHMEAVFGGSPQDIGMQQGVGGVSPFT
                     TDCGIIENSIVFTFTSVFPQNAQTICEVMSQEVAHSYGLDHEMLASDPMTYQSYNGKR
                     TFKDQTVSCGEYSNRQCGIGGSVCRPNQNSVQLLTQRLGLADAIAPTMTITSPADGAT
                     VMPGFEVTATATDNVAVTQATFFIDDVMITQTPGAGPYSFPTDPALAEGSHSIAIEVT
                     DGRNPLKQTIHVTVAKDSGSGSGSGSGSGSGSDGDGGGGGGGGGDDDGGLSGGCSSTQ
                     GVGFAIGLFFLAHMMIRRRRYV"
     gene            complement(4877..>5499)
                     /locus_tag="VFV99_04665"
     CDS             complement(4877..>5499)
                     /locus_tag="VFV99_04665"
                     /inference="COORDINATES: protein motif:HMM:NF041144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="expansin EXLX1 family cellulose-binding protein"
                     /protein_id="HEX5058627.1"
                     /translation="NCNDTPAEAMGDGTYYDATGAGNCGFDASPNDLLVAAMNAADYN
                     NAAWCGACLAVTGPMGSVTVRVVDQCPGCAHGDLDLSQEAFGMIAPLSAGRVAITWHE
                     VACNVSGPIAYHFKDGANAYWTAIQIRNHRYPIASVEAMSPVATSGWQEIPRLSYNYF
                     VEDAGLGAGPYTLRVTDTRGHVLEDPGIALGDNITRTGADQFPLCP"
BASE COUNT          967 a         1790 c         1777 g          965 t
ORIGIN      
        1 gaacgactgc gctcgtttct gcgcgacgtg ttcgcggtgt tcgagtcgca tcgcaagttc
       61 atcaaggttg cggtcgagac cgagcacttc aaacaaacac cctcgacgac ccagaatgac
      121 ctcgcgagcg cgatcggcga gatcgtcaag gcgggtgtga aggagaaggt gctgtcgccg
      181 cggctggccg agatgctgcc gttcgtgatc tcgggaaccc tcaagggcta cggcttgcgc
      241 cggatcggtg agaacggtgc gctcgaagcg gcggatgccg aagcactggt cacgatcttc
      301 ctcgacggtg cgcgtaaatg agcgcgaccc cggcgccggt aaacaagtgg gtcgtcgcgg
      361 tctcgatcgc gttcggctcg ctgatggcaa cgatcgacac ctcgatcgtc aatgtcgcga
      421 tgccgcagat ccgcggcgag ctcggtgcgt cgctgcaaga gatcacgctg atcacgaccg
      481 gctacatgat cgcgatggtg ctcgtcatgc cgctgacggg ctttctcggc gcgctattcg
      541 gtcagaagcg cgtctatctc gcgtcgctcg cgatttttac gatcggctcg gtgctgtgcg
      601 gcactgcgcg ttcgctcgag atgctcgtcg tgtggcgcat cctgcaaggt ctcggcggcg
      661 gagcgctgca gccgacacag caagcgatct tgcgccagac gttcccgccc gaagaacagg
      721 gcatggcgat ggcgctgttc tcgatggtga tcatggtcgg cccggccgtc ggccccgtgc
      781 tcggcgggtg gctcaccgat ggtttctcgt ggccgtggat cttcttcgtc aacctgccgg
      841 tcggcctgct cggcatctac ctgacgatgc gcaacgtgca cgagcccgag gacgtgcgtg
      901 tcgagaaccg cgcccgcgcg cagatcgtga agaagcactt cgactacgcc ggcatcatat
      961 tgatgggcgt cgcgatcggc ggactgcagt acgtactcga agagggctcg gccgccgact
     1021 ggttcgactc gacgaagatc gtggtcgcga cctacatcgc tggtatctcg ctcgcggcgt
     1081 tcatcattcg cgagctgacg gcgaccgcgc cggtcgtcga tctgcgcatg ttccgcgatc
     1141 ccacattcgc atcgggcacc gcgatcgcgg gcctcgtatt cgcgatgctg atgggctcga
     1201 tgttcctgct gccggtgttc acgcaggaaa tgctgcacta ctcggcaacg cagtccggca
     1261 tcgtgctgct gccgcgcacg atcgcgatga tgatcgcgtc gccgttcgtc ggcaagctct
     1321 acaaccgaac gcgccctgcg tacctcgtcg cgttcggcat gctggcgacc gcgctcggtt
     1381 gctggatgca ggccgacatc acgctgcaca cgtccacgag cgatctgttc tggcctctcg
     1441 cggtcaccgg tattggcttc gcgttcctgt tcgtgccgct caccaccgct gcgctatcca
     1501 acgtgccgcg cgcgaacctc gcgggcgctg ctggcgtgaa cagcttcgtg cgtcaggtcg
     1561 gcggctcgat cggcctgtcc atcttcgcca cgatgttcca gaactacggg gcgcgcgcac
     1621 aggccgcgct cgcctccggt atcagcgtgc tgcggcccga ggtcgcggcg caggtcgatg
     1681 cgggcctcac cgctcacgct gcatatggcc gcaccgcgct cgaaggcgtc gtgatcggct
     1741 ttgacaagat gttcctcctt cagacgtttg cgttcgtcat cgtgttgccg ctgctgctgt
     1801 tcttgcgcgt gcaacgtcaa gcggcgccgg cgcacgtcga actgcccgcg gagtaaaccc
     1861 atggaagcgt cagtcaaacc taaaccacgt cgcggcaagc gcgcgtatct cattctcgga
     1921 gtcatctcgc tcattgtcgc gggactctgg cttggccatc gcttgtggac ccgcgggaaa
     1981 caatccaccg atgacgcgca ggtcgaagcc gacgtcacac cgatcgcatc gcgtgtggcc
     2041 ggcacgatcg ctgcgacgaa ggtccacgac aaccaggccg tgaagaaggg cgacgtgctc
     2101 ttcgagatcg atccggcgaa cctcgacgtc gaggttctgc gcaccgaggc cgacctcgaa
     2161 gcggcgcgcg cgcagctcgg cgcagcccag gcgcaggttg cgatcgtgca ctcgtcctcg
     2221 aagggcggcc tgtcgtcggc gcaagctgcg ctgtccggcg ctgccgcaag cgcacgcggt
     2281 gccgccgact cgattcgcgc cgccgatgca gcagtcgcgc gcgcccgcgc cgatctgcag
     2341 aacacgcaga gcgagctcgc tcgcgtacag gcgctgttcg acaaggaagc cgtcactcgt
     2401 caggagctcg agcacgcgca gcacgcccgc gatgtcgcgc aggctgcact caacgcggca
     2461 accgcgcagc tcgatggagc gcgcgaccag cgcaacatgg cccaggcccg cgtgagtgaa
     2521 gccgagggca aggtcgcgca gagcgcgccg gtcgatcagc aggtcgcggc cgcgcaagcc
     2581 gctgccgacc tcgccacggc gcgggtaaaa gccgccgagg cggcagtcgc caaggcgaag
     2641 ctcgatcgcg cacacgccac gatcgtcgcg cccgtcgacg gcgtcgtctc gaagctgggt
     2701 gcgcacgtcg gccagtcggt caacatcggt cagccgctgc tgatgctcgt gccgaacgag
     2761 acctacgtcg tcgcaaactt caaggagagc cagatcgctc acatgaagcc gggcgacaag
     2821 gtcgacatcg agctcgatgc attcgaaggt gacttcgcgg gtgtcgtcga gaccctgtca
     2881 ccggccaccg gcgcgcggtt ctcgctgatc cagcccgaca acgcgaccgg caacttcgtg
     2941 aaggtcgtgc agcgcgtgcc ggtgaagatc aaatgggcgg acacgccgaa ggtctcggtg
     3001 cgacccggtc tgtcggccga ggtcaccgta cacgtgggcg actgaggccc ggcgcggtac
     3061 gcttcagcca tgcggtacgc agcgctcgtg ctcgtgatgc tcgtcgggac ggccaccgcc
     3121 gaccccgcgg cggacgaaac ggcggcctac gagaacgcga aacctgtctt cacaaaatat
     3181 tgcgcgagct gccatacgaa ggccggcgcg aaggcgaccg cgaagaagct cgatcacttc
     3241 gatatgacca cgtacccatt tggtggccat cacgcgacga cgattgccgc caccatgcgg
     3301 aaggtgctcg ccatcaaggg cggcaagccg acgatgccct ccgatcgaaa gggcgcagtc
     3361 aaaggtgacg agctcgctct catcgccgcg tggtgcgatg cattcgacgc tgcacataaa
     3421 gcaaactcga cgcatcacca tcactgactc acaccagtga tgcgaatcgt ttacggagaa
     3481 caacgccccg atcagggtga cagctcggcg cactgaatgt gttcgtgcac tagttgtgca
     3541 acgcgcgctt tcttgcgaat tctcgacgat gaagcgctgc gcgagttgcg tggcacgcgg
     3601 cgtgattagt gcgaccgcat gcgcaccgcg acgattctga cagcccttct cgtcgtcgtt
     3661 ccttctgtcg ctgcggcgga agatcagatc acggtcgaca accaagtacg accggatctg
     3721 ttcgcatctc cgcctaccga cacccacatc acgttcgtac gcaagatgtt gccggcgacg
     3781 aacgcgccga tcgcggccgt cgcacagagc cgcatcatct acctcaacaa gaacggcgtg
     3841 acgctcacgc cgggcaacga tgactcgcgc acgaaccggt cgtcgatcat ttcgtcgcag
     3901 cgaaccgttc cggcatggaa cgcgagcgcg acactgtggt ccgacacggt caagtgcatg
     3961 acggacatgt tctcgcggtg ggacgttcag gtcaccgacg tcaatccggg caacgtgccg
     4021 cacatggagg ccgtgttcgg cggcagcccg caggacatcg gtatgcagca aggcgtcggc
     4081 ggcgtgtcgc cgttcaccac ggactgcggg atcatcgaga actcgatcgt cttcacgttc
     4141 acgagcgtgt tcccccagaa cgcgcagacg atctgcgagg tcatgtcgca agaggtcgcg
     4201 cacagctacg gcctcgacca cgagatgctt gcgtcggatc cgatgacgta ccagtcgtac
     4261 aacggcaagc gcacgttcaa ggatcagacg gtgtcgtgcg gcgagtactc gaatcgccag
     4321 tgcggcatcg gcggctcggt gtgccgtccg aatcagaact cggtgcaact gcttacccag
     4381 cggctcggcc tcgccgacgc gatcgcgccg acgatgacga tcacatcccc cgcggacggt
     4441 gcgaccgtga tgccgggatt cgaggtcacg gcgacggcga ccgacaacgt cgcggtcacg
     4501 caggcgacgt tcttcatcga tgacgtgatg atcacgcaga cgccgggcgc cggcccgtac
     4561 agcttcccga ccgacccggc actcgccgaa ggttcgcact cgatcgccat cgaggtcacc
     4621 gacggccgca acccgctgaa gcagacgatc cacgtgaccg tcgcgaagga ctcaggcagc
     4681 ggcagtggta gcggtagcgg cagcggcagt ggcagcgatg gcgacggtgg cggtggcggc
     4741 ggcggtggtg gcgacgacga cggcggccta tcgggcggtt gttcgagcac ccagggtgtc
     4801 ggctttgcga tcggcctgtt cttcctcgct cacatgatga ttcgccgccg gcgctacgtt
     4861 taggtggcgg tcctcttcac gggcagagcg gaaactggtc ggcgccggtc cgcgtgatgt
     4921 tgtcgccgag cgcgatgccc ggatcttcga gcacatggcc gcgagtgtcg gtgacccgca
     4981 gcgtataggg accagccccg aggcccgcgt cctcgacgaa gtagttgtac gagaggcgcg
     5041 gaatctcctg ccatccgctc gtggccacag ggctcattgc ctcgacgctc gcgattggat
     5101 agcggtggtt gcggatctgg atcgcggtcc agtacgcgtt cgcgccgtcc ttgaagtgat
     5161 acgcgatcgg accgctgacg ttgcacgcca cctcgtgcca tgtgatcgcg acgcggcctg
     5221 ctgacagcgg cgcgatcatc ccgaacgctt cttggctgag atcgagatcg ccgtgcgcgc
     5281 agcccgggca ttgatcgaca actcgcaccg tgacactgcc catcggtccc gtgaccgcga
     5341 ggcacgcgcc gcaccatgca gcgttgttgt agtcggcggc gttcatcgcc gcgacgagca
     5401 ggtcattcgg tgatgcatcg aacccgcaat tgccggcacc ggtcgcatcg taataggtgc
     5461 catcgcccat cgcttccgcg ggtgtgtcgt tgcagttgc
//
LOCUS       DASUFX010000123        14145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1549082, whole genome shotgun sequence.
ACCESSION   DASUFX010000123 DASUFX010000000
VERSION     DASUFX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549082"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1236
                     /locus_tag="VFV99_04670"
     CDS             16..1236
                     /locus_tag="VFV99_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5058628.1"
                     /translation="MSVYQHAGITILTKHDARARGERKSRIALVLAGGAITGGAFKAG
                     GLRALDELLRPAMTLNDFDQFIGLSAGSVLASALAAGIDAEELFKITIGKSERFSQLQ
                     AYDFMRPNVREPAERIALFIQKVAEILGAYVSGREDPRTGQPFSAKQTALKLVSVLAR
                     LAPTGVFDPNGVRDYLRRQIKELGMPDDFRELQRLRGKELYVTAVDINTGTQVVFGPR
                     EHYSAVRVSDAVSASCALPVWYRTARVRNPRYREPTERRWLDLADGGVVRTANVGTAL
                     RAGADLVVVYNPFTSIRYTAPDRSLYEHGAYALASQLFRTVLGSRLDIGKEKIVVDPN
                     VRADIVYIEPRSEDLDFFAMNPLNFWSKQRAAQHGYDSVRVALAENHEPLREVFERHG
                     VELSGGRLLTKVVA"
     gene            1233..1613
                     /locus_tag="VFV99_04675"
     CDS             1233..1613
                     /locus_tag="VFV99_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058629.1"
                     /translation="MIADPPVIARVRAQLVDQVQPPLVVSTTSQQYYFDEEHAAYCEI
                     EVKEGEAFAAVVVRGSLPSEEFVVRVWHRYADEVWLVDSVEQVMFVVPREGEIRVFEI
                     GETMRSVRVPDVHIAVATVFGIVN"
     gene            complement(1606..5037)
                     /locus_tag="VFV99_04680"
     CDS             complement(1606..5037)
                     /locus_tag="VFV99_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5058630.1"
                     /translation="MSWEPTALFEALDDAVSVFDADLRWVYANAAAAKVSGIVPADLI
                     GKRVWDVFPDGKDTEFYIAFERTRATGASQIAESYYAPLDRWFRIQTYRVGEYIFVVS
                     RDITEQKQGEARLLVLSRASHAFARVAELEPMFEVIARTLAELVGDACIVRMLEHGKL
                     RPVAVHHPDPERQTVLRQFFATPFGLREGLSVKVLESGEPLLVEHLDRAALRNTFTTE
                     SHRMRVESGGVHSVMASALSHGAQRVGVVTLLRDRTARPYTRGDLSLLQDLADRASMA
                     VSRARLYEDAQQERRRALAAAAASRSFSAAERDTHAVLDLLARATATEVGEIAVAAVV
                     SEDGQYLQPVGCHANSEIADEIRAKLTARAPVAGSMSERVMATRKPLRMANLDVEAFA
                     ASAQEQYRGMVHRFQPRSFMLVPIIRSGHALGTLFVSRQDSFEPYSDDDERLLLELAE
                     RAGIAVSNGRVLEAERLARLSAERVAEHTRRLQTIGAQLSHRRSPREVAETILRESTE
                     MLGGDAAGTIWLLGDSETKLEVLAHVGLGVPEQFTTLPLDSDIPLVSAIRSQTPLFIA
                     NLDEYTARFPSSARRVAPAAPAEFAVACVPLVNEGRAIGGFSFMFPHAHVFLPDERTF
                     LEVIATQCAQAIDRAQLLAQEQAATAALAETNRTLNALIHASPAAIIMFDLDGAIRLW
                     NPAAERIFGWAAADVLGKPWPLLDKGQQAERDSKIAQIARGQELRGFETKRPRRDGTQ
                     IDVAVWAAPVQRPDGEAQVVALFVDITDRKHAEELARAADRRKDEFLAMLGHELRNPL
                     APILTALELMRIRKETGGEHERATIERQTRHLVRLVDDLLDISRITRGTIELRKSRVD
                     LGAAIAHALEMASPLLEQRGHHVSIAAPRGLVYVDGDEIRLAQVFQNLLTNAAKYTPT
                     GGSITARLTVRDNHALAEIEDNGVGIAPSVLPTIFEPFVQGAQKLDRSQGGLGIGLTL
                     VRSIVDLHGGRVEAHSAGPGHGSRFTVTLPQARDHLRDVQGTPQGMVPLRGIKRRVLV
                     VDDNRDAAEMLAELLRAAGHEVAVAYDAPTALSTLPSFKPEIALLDIGLPVMDGYDLA
                     RKLKAALPNTPRLVAITGYGQEHDRRRSEEAGFDEHLVKPVQAAQVLAAVDAVAS"
     gene            complement(5096..5434)
                     /locus_tag="VFV99_04685"
     CDS             complement(5096..5434)
                     /locus_tag="VFV99_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058631.1"
                     /translation="MPGPGPQDRLGPQITILASMAPAGGSDGTYHFIDDNIWDIRVID
                     PNGHIVFENDKLKGGGGDKVVLGTGQIDQFTFRMPFGSAEGTYTVEAQLLQSGVQPIV
                     TAVFEYGWAQ"
     gene            5709..6383
                     /locus_tag="VFV99_04690"
     CDS             5709..6383
                     /locus_tag="VFV99_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058632.1"
                     /translation="MSAAFASATFAQPGPLPAADSAKQGTDLYNAGDYAGAVAPLQRA
                     VEMEPNNFEYRFMLAQALRQSGNCAEAMPQYKQLEASAPPERANDVKTAMAGCPSTDI
                     KQPDAVPAPPPPPPPEPVVKSGLSRGNALMLMGAGAGIATSVCLLFAASNDASDADAA
                     AKYDDHDRLSTRSEREYIGAAVAGGVGVVLGVIAFRRINSSNEGTQLSLRPRSGGGTL
                     VLERSW"
     gene            6437..7465
                     /locus_tag="VFV99_04695"
     CDS             6437..7465
                     /locus_tag="VFV99_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058633.1"
                     /translation="MRLAFLSALGLLVSLPVGCGDDGVSVSADENNFCGEIAEVGCHN
                     FYQCCSEGEIENLLNVSEPRTEAQCRDDLTKLCDRLGNQLTDSIKAGRVSFDAQIMND
                     CLNAIVAPSDSCAAIDTALPWKQACMTSAFVGKVATGAACFFNHDCAGAPDSFCGPDQ
                     KCQQKPTAGFPCGTGCASQFYCGPSGTCVAKVSTGGPCTSSDACTTDLFCDFSQNPTM
                     GMCAAKGPGGTACLGNDGCASGSCIPGRCMGTNFTCYQDTQCSGHCANSTLSCTTSAT
                     CGTGTCSVSSISCTTQTTCDNTGQTGDTCVFPVLCLPGDCVGDPVCTNALTTVDYCTG
                     TASALPVL"
     gene            7477..9468
                     /locus_tag="VFV99_04700"
     CDS             7477..9468
                     /locus_tag="VFV99_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HEX5058634.1"
                     /translation="MRSKHLCAIALLAAACGDDGATSRDGGVDVQLIDAPTSVPMITS
                     FVPSPSQIPANTPTQVTFTWTYLMEPTLPDPTCSIDNGIGAVMRGVPVTVTIAGTTLL
                     TLTCTNSAGAGSKQVVLAVPPAAPILATFTATPAPIMPNAATSVTFSWTYSNSPSPAP
                     MCTIDGVAATSGTAMQLTLSQARTYRLRCSNTQGFAIRDVTVPVNECAGGTHDCNAHA
                     TCNDTVEGFTCSCNTGYVGNGDVCSQQNVNCVAGCGANATCTTATNACTCNVGFVGDG
                     VTCTRERLMFVTSAFGTGNLSSWSGAVGTGLAAADSICQAEAATLGLTGTKFVAWMSD
                     SNTDAYCHIHDLTGKKATGCGLGAGNLPVAAGPWARADATRSPAAPAIDKWLAPMRQT
                     FTPVTFTAAGNDVSATTPFFLYTGTDDSGVFTGTACTDWTTTAGTGAMGDISGGGTAW
                     TDQGTDPSCATSGRLRCVEVGTGPALPSRHPANAKRGFVTSVSGTGAFSTWADAQGSS
                     GITAADEICRSRARFAGYANAASFKAWASYSTVSAPNRFTYSGPWYRPDGIQIGTRTT
                     LTTGRILAPMYQTEANDYASGNAETGSVWTGTTAGGSYYTSSGNCSSWGFTTSTAIIG
                     RTDMADFRWVAVGSSSSVPTYTNSCSATNYRLYCLDDSP"
     gene            9596..11083
                     /locus_tag="VFV99_04705"
     CDS             9596..11083
                     /locus_tag="VFV99_04705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058635.1"
                     /translation="MSPGTQVGAYRIVKQIGEGGMGSVWLAEHAMLGRRAAVKLLHPS
                     YSSHQEIVQRFFNEARAATSISDPGIVQIFDFGNQPDGQAYIVMELLEGEPLDTRLER
                     QGRLPIDETLRIMRQVASSLGAAHARGIVHRDLKPENIFMVRDPEVVGGERAKLLDFG
                     IAKLGEQSGVQTQTNMIMGTPAYMSPEQCRGAKHIDARSDVYALGCVMFTLLTGRPPF
                     TAEGHGDWIVMHITQPAPRLASVLPHASAMLDQLVAKCLEKDPNARFANGAELAGALN
                     QLVTHMSQPGMPIAAHAAVTIAAKPQTTLRSAAAQSAAVTGKQSSTLAIVGGLVVLLA
                     IGGGIAFYATRDKGEPDGQAQASAPTPMPPKAEPPKPEPLPPPSPAPAPPEPSPPAEA
                     KPETVDIRIESNPSGAAVFVAEEKVPRGKTPYTLSIKKSDPAIEIKLVATGYAPKKAK
                     VSPATDTSIAVPLTKARRGTTATQTPSSPVSDDSDETMNPFNKKK"
     gene            11106..11921
                     /locus_tag="VFV99_04710"
     CDS             11106..11921
                     /locus_tag="VFV99_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058636.1"
                     /translation="MVVAALILSSGLVLAQSGEKAAEEWFNKGNVAYDLGRYDEAADN
                     FTKAYEAWQRPEFLYNIAQAYRLGGNCAKALHFYKRFKTLKEKDTLAPLPAKKREEVD
                     RFISELTDCAAKGQSSANVPPDTTDRPPQSPPATTPTPTTIQTAAAPPAEPEEPEQPV
                     DTPARSSNVVPIAVGAGAVVLFGAALGLELWARSTYSDAKAENDPATQESLWHSANTK
                     RYTADGLAVGGAACAGIAVWLFIRNRNNPSEKEVTIAPIGGPGLAGIGIAGSY"
     gene            12698..13543
                     /locus_tag="VFV99_04715"
     CDS             12698..13543
                     /locus_tag="VFV99_04715"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX5058637.1"
                     /translation="MRQLGTSADDIARGVAIDPNGNIYVAGTTRGNLDGTSAGGYDGF
                     VAKYNGSGALQWVRQFGTTADEGGSGAIADPSGNVYVTGYTSGSLDGTNAGATDIYLV
                     KYDTTGVKQWTRQLGTSDYDAGDAIAIDGMGNIFIAGETMGGLDGNTSAGGQDLVLVK
                     YNDAGSKLYTRQFGSTANDVGYGIAMNLGGEAYVTGNTAGGLEGNTNAGMQDLYVVKY
                     ALNGTRVWIRQMGSASYDVGLGITVLSNDVVVFGSTGGGVDGNTNAGATDCIIVKYQQ
                     DGRKR"
     gene            13634..13861
                     /locus_tag="VFV99_04720"
     CDS             13634..13861
                     /locus_tag="VFV99_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058638.1"
                     /translation="MADALAQLRGVAACRGEYWARCVAERVVRTRLWPAYDGRCKSIA
                     HDKVRDLGRDERLREELARLCASWAARWWAH"
     gene            complement(13858..>14145)
                     /locus_tag="VFV99_04725"
     CDS             complement(13858..>14145)
                     /locus_tag="VFV99_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058639.1"
                     /translation="RVRMVARDGGGVGTIAEDQPLVFDLAADDKNLYFVTAYSDVYRI
                     KQVSRFGGPVHMLACGHHQGQALYLTVAGDYVYWSDSFSLQRIEKFDPTFR"
BASE COUNT         2540 a         4486 c         4717 g         2402 t
ORIGIN      
        1 gtatagtgcc gcatcgtgag cgtttatcag cacgcgggga tcacgatcct gaccaagcac
       61 gacgctcgcg cgcgcggtga gcggaagagc cggatcgcgc tcgtcctcgc cggcggcgcc
      121 atcacgggtg gcgcgttcaa agcaggtggc ttgcgggcac tcgacgagtt gctgcgcccc
      181 gcgatgacgc tcaacgactt cgatcagttc atcgggctgt ccgccggttc agtgctcgcg
      241 agcgcgctcg ctgccggcat cgatgcagaa gagctgttca agatcacgat cggcaagtcg
      301 gagcggttca gccagctgca ggcctacgac ttcatgcggc cgaatgttcg cgagcccgcc
      361 gagcggatcg cgctgttcat ccagaaggtc gccgagatcc tcggtgccta cgtgtcgggc
      421 cgagaggatc cgcgcaccgg ccagccgttc tcggcaaagc agaccgcgct caagctcgtg
      481 agcgtgctcg cgcggctcgc gccgaccggc gtattcgatc cgaatggcgt gcgcgactat
      541 ctgcgccgcc agatcaaaga gctcggcatg cccgacgact tccgcgagct ccagcgcctg
      601 cgcggcaagg agctctacgt caccgccgtc gacatcaaca cgggcacaca ggtcgtgttc
      661 ggaccgcgcg agcactattc ggcggtgcga gtctccgatg cggtgagtgc atcgtgtgcg
      721 ttgccggtct ggtaccgaac tgctcgcgta cgtaatccgc ggtatcgcga gccgacggag
      781 cggcgctggc tcgatctcgc cgatggcggc gtcgtgcgca ccgccaacgt cggaacagcg
      841 ctcagggcag gcgccgacct cgtcgtcgtt tacaacccgt tcacctcgat ccggtacacc
      901 gcgcccgatc ggtcgctcta cgagcacggc gcgtacgcgc tcgcgagcca gctgtttcgc
      961 accgtcctcg gctcacggct cgacatcggc aaggaaaaga tcgtcgtcga tccgaacgtg
     1021 cgcgccgaca tcgtgtacat cgagccgcgc agcgaagacc tcgacttctt cgcgatgaat
     1081 ccgctgaact tctggtcgaa gcaacgcgca gcacagcacg gctacgacag cgtgcgcgtc
     1141 gcactcgccg agaaccacga gccgttgcgt gaggtgttcg agcgtcacgg cgtcgagctg
     1201 tcgggcggcc gattgctcac gaaggtcgtc gcgtgattgc agatcctccg gtgatcgcgc
     1261 gcgtacgcgc ccagctcgtg gaccaagtcc aaccgccact cgtcgtcagc acgacgtcgc
     1321 agcagtacta cttcgacgaa gagcacgcgg cgtattgcga gatcgaggtg aaggagggcg
     1381 aggcgttcgc cgccgtggtc gtgcgcggct cattgccgtc ggaggagttc gtcgtgcggg
     1441 tgtggcatcg ctacgcggac gaggtgtggc tcgtcgatag cgtcgaacag gtgatgttcg
     1501 tcgtgccgcg cgaaggcgag attcgcgtgt tcgagatcgg cgagaccatg cgctcggtgc
     1561 gcgtgcccga cgttcacatc gcggttgcga ctgtgttcgg gatcgtcaac tagctactgc
     1621 gtcgacggcg gcgagcacct gcgcggcctg cacgggcttg acgaggtgtt cgtcgaatcc
     1681 agcttcttcg ctgcgacgac gatcgtgctc ctggccatac ccggtgatgg caaccagccg
     1741 gggcgtgttc ggcaacgcgg ccttcagctt gcgcgcgagg tcgtagccgt ccatcaccgg
     1801 caacccgatg tcgagcagcg cgatctccgg cttgaacgag ggcagggtgc tgagcgcggt
     1861 gggcgcatcg tacgcgacgg cgacttcgtg gcctgcagcg cggagcagct cggcgagcat
     1921 ctcggcggcg tctcggttgt cgtcgacgac gagcacgcgg cgtttgatgc cgcgaagcgg
     1981 aaccatgcct tgcggtgtgc cctggacgtc gcgaaggtga tcgcgcgcct gggggagcgt
     2041 gaccgtgaag cggctgccat gccctggtcc cgcgctgtgc gcctcgacgc gaccgccgtg
     2101 caggtcgacg atgctgcgca cgagcgtgag gccgatgccg aggccgcctt gcgagcgatc
     2161 gagcttctgt gcgccctgta cgaacggctc gaagatcgtc ggcagcacgc tcggcgcgat
     2221 gccgacaccg ttgtcctcga tctcggcaag tgcgtggttg tcgcgcacgg tgagccgtgc
     2281 cgtgatcgat cctccggtag gcgtgtactt cgcggcgttc gtcaataggt tctgaaacac
     2341 ctgcgcgagc cggatttcgt cgccatcgac atacacgagt ccgcgcggtg cggcgatcga
     2401 gacgtgatga ccgcgttgct cgagcagcgg actcgccatt tcgagcgcgt gtgcgatcgc
     2461 tgcgccgaga tccacgcggc tcttgcgcag ctcgatcgtg ccgcgcgtga tgcgcgagat
     2521 gtcgagcaag tcatcgacga gccgcacgag atggcgcgtc tgccgctcga tcgtcgcgcg
     2581 ctcgtgttcg ccgccggtct ctttgcggat gcgcatcagc tcgagcgcgg tgaggatcgg
     2641 cgcgagtggg ttgcgcagct cgtggccgag cattgcgagg aactcgtcct tgcgacggtc
     2701 ggcggcacgc gcgagctcct cggcgtgttt gcgatcggtg atgtcgacga acagcgcgac
     2761 gacctgggct tcgccatccg ggcgctgcac cggcgcggcc catacggcga catcgatctg
     2821 ggtgccgtcg cgtctcggcc gtttcgtctc gaagccacgc agctcttggc cgcgtgcgat
     2881 ctgggcgatc ttactgtcgc gctccgcttg ttggcccttg tcgaggagcg gccacggttt
     2941 gccgagtacg tcggcggcgg cccagccgaa gatccgctct gcggcaggat tccagagtcg
     3001 gatcgcgcca tcgagatcga acatgatgat cgcagccggc gatgcgtgga tgagcgcgtt
     3061 gagcgtgcgg ttggtctcgg cgagcgccgc ggtcgccgct tgctcttgtg cgagcagctg
     3121 cgcgcgatcg atcgcctgcg cacattgggt cgcgatcact tcgaggaacg tacgttcgtc
     3181 gggaaggaag acgtgcgcgt gcgggaacat gaacgaaaag ccgccgatcg cgcggccctc
     3241 attgaccagc ggcacacatg ccacagcgaa ctcggcgggg gccgccggag cgacacgcct
     3301 ggcggacgac ggaaagcgtg ccgtgtattc gtcgaggttc gcgatgaaca gtggggtctg
     3361 cgatcgaatc gcgctgacga gaggaatgtc ggagtcgaga gggagcgtcg tgaactgttc
     3421 cggcacgccg aggccgacgt gcgccaatac ctcgagcttt gtctcgctgt cgccgagcag
     3481 ccagatcgtg ccggcggcat caccgccgag catttcggtg ctctcgcgga ggatcgtttc
     3541 ggcgacctcg cgcggcgagc ggcggtgtga cagctgcgcg ccgatcgttt gcaagcgtcg
     3601 ggtgtgctcg gccacgcgct cggcggatag tcgtgcgagg cgctcggctt cgagcacgcg
     3661 gccattgctg accgcgatac cggcgcgttc ggcaagctcg agaagcaacc gctcgtcgtc
     3721 gtcgctgtac ggctcgaacg aatcctgccg gctcacgaac agcgtgccga gtgcgtgacc
     3781 ggagcggatg atcggcacga gcatgaagct gcgaggctgg aacctgtgca ccatgccgcg
     3841 gtattgctcc tgcgcgctcg cggcgaatgc ctcgacgtcg aggttcgcca tccgcagtgg
     3901 cttgcgtgta gccatcacgc gctccgacat cgagccggcg actggtgctc gtgccgtgag
     3961 cttggcgcgg atctcgtccg cgatttcgct gttcgcgtga cagccgaccg gttgcaggta
     4021 ctgaccatct tcgctcacga ccgctgcaac cgcgatctcg ccaacctcgg tcgccgtcgc
     4081 gcgcgcgagc aggtcgagga ccgcgtgcgt gtcgcgctcg gcagcgctga acgagcgcga
     4141 ggccgccgct gccgccagcg cacgacgccg ctcttgttgc gcatcctcgt agaggcgggc
     4201 gcgcgacact gccatgctcg cgcggtccgc gaggtcctgc agcagcgaca ggtcgcctct
     4261 cgtgtacggc cgtgccgtgc gatcgcggag cagcgtcacg acaccgacgc gctgagcgcc
     4321 atggctgagt gcactcgcca tcaccgaatg cactccgccg gactcgaccc gcatgcgatg
     4381 gctctcggtc gtgaacgtgt tgcgcagcgc cgcgcggtcg aggtgctcga cgagaagcgg
     4441 ctcaccgctc tcgaggacct tcacgctcag accttcacgc aagccgaacg gcgttgcgaa
     4501 gaactggcgg agcaccgtct gccgctctgg atccgggtga tggacggcga ccgggcgcaa
     4561 cttgccgtgc tcgagcatgc gcacgatgca ggcatcgccg acgagctcgg cgagtgtgcg
     4621 agcgatgacc tcgaacatcg gctcgagctc cgctacgcgt gcgaacgcgt gagaggcgcg
     4681 cgacagcacg agcaatcgcg cctcaccttg tttctgctcg gtgatatcgc gagagacgac
     4741 gaagatgtac tcgccgacgc gatacgtctg gatgcgaaac cagcgatcga gcggcgcgta
     4801 gtagctctcg gcgatctggc tcgcgccggt cgcgcgcgtg cgctcgaacg cgatataaaa
     4861 ctcggtgtcc ttgccgtcgg gaaagacgtc ccagactcgt ttgccgatca ggtccgccgg
     4921 cacgatgccg ctgaccttcg cggcggccgc gttcgcatag acccagcgca ggtcggcgtc
     4981 gaatacgctg acggcatcgt cgagagcctc gaacagcgcg gtcggctccc aggacatctc
     5041 cgacgatttc agccccagac cgggaaaccg tcaactgccc gggccggcca agtggtcact
     5101 gcgcccagcc gtactcgaac accgcggtga cgatcggctg cacgccggat tgtaggagct
     5161 gcgcttcgac cgtgtatgtg ccctcggcgc taccaaacgg catgcgaaac gtgaactgat
     5221 cgatctggcc ggtaccgagg acgaccttgt cgccgccgcc gcctttcagc ttgtcgttct
     5281 cgaacacgat gtggccgttc ggatcgatca cgcggatgtc ccaaatgttg tcgtcgatga
     5341 agtgatacgt gccgtcgcta ccaccggcgg gcgccatgct cgcgaggatc gtgatctgcg
     5401 ggccgagccg atcctgtgga ccaggacctg gcatgtgatt ctcccaatac gcttgggcat
     5461 ccgtggcgac cgctaccaac agcacccctt aagtagagca cgctcacatt agatggagtg
     5521 cgcggatgta tgcggtggtc acttcactaa gtcccgcgtg ccttccgatg ccgttcgctg
     5581 cgtagcgact cacacgcgcg aaccgcgcga gtactgatct ttcggggtat cgtggacgtt
     5641 gtagtgcgtg catggggaag cacgcaggag gaggcttcgt gggatcgaga ctggctttgg
     5701 ccgcggtgat gagtgcggca tttgcgtctg cgacgttcgc gcagcctggc ccgttgccgg
     5761 cggcggatag cgccaagcag ggaacggatc tctacaacgc tggtgactac gcgggcgccg
     5821 tcgcgccgct gcagcgcgcc gttgagatgg agccgaacaa cttcgaatac cgcttcatgc
     5881 tcgcgcaggc gctccggcaa tcgggcaact gcgcagaggc gatgccgcag tacaagcagc
     5941 tcgaagcgag tgcaccgccc gagcgcgcga acgacgtcaa gaccgcaatg gccggatgcc
     6001 cgagcacgga catcaagcag ccggacgcag tgccagcgcc gccaccacca ccgccgcccg
     6061 agcctgtagt gaagagcggc ttgagccgcg gcaatgcgct gatgctcatg ggcgcgggcg
     6121 ccggcatcgc gactagcgtg tgcttgctgt tcgccgcgag caacgacgcc agcgatgcgg
     6181 acgccgcagc gaagtacgac gatcacgaca ggctgtcgac gcgctccgaa cgcgagtaca
     6241 tcggtgctgc ggtcgccggc ggcgtcggcg ttgtactcgg tgtgatcgcg ttccgccgca
     6301 tcaacagctc taacgaaggc acgcagctgt cgctacgccc acgttcaggc ggcggcacgt
     6361 tggtgctcga gagatcctgg tgagacatcc gttttcccgt ccgtcgtcac gcacaatcga
     6421 tcttggggga gatgccatgc gtctagcttt tctgtctgct cttggtttgc tcgtcagtct
     6481 gcccgttggc tgcggcgatg acggcgtcag cgtcagcgcc gacgagaaca acttctgtgg
     6541 cgagatcgca gaggttggtt gccacaactt ctaccaatgc tgctctgagg gtgagatcga
     6601 aaatctgctc aacgtcagcg agccgcgcac ggaagcgcag tgtcgtgacg atctgacgaa
     6661 gctctgcgat cgcctcggta accagctcac tgactcgatc aaagcgggtc gcgtgtcgtt
     6721 cgatgcgcaa ataatgaacg actgcctcaa cgcgatcgtc gcgccgtcgg attcatgtgc
     6781 agcgatcgac accgcgctgc cgtggaaaca ggcgtgcatg acgagcgcgt tcgtcggcaa
     6841 ggttgcgacc ggtgcggcct gcttcttcaa ccacgactgc gcgggcgcgc ccgatagctt
     6901 ctgcggcccg gaccagaagt gtcagcagaa acccacggcg ggtttcccgt gcggcaccgg
     6961 ctgcgcgagc cagttctatt gcggacccag cggaacgtgc gtcgcgaagg tcagcacggg
     7021 cggaccgtgt acgtcgagcg acgcgtgcac gacggatctg ttctgtgact tcagccagaa
     7081 cccgacgatg ggtatgtgcg cggcgaaggg tccgggcggt acggcgtgcc tcggcaacga
     7141 tggttgtgcg tcgggctcct gcatccccgg ccgctgcatg ggcacgaact tcacgtgcta
     7201 ccaggacacc cagtgctcgg ggcactgcgc gaacagcacg ctgtcgtgta cgacgtcggc
     7261 gacgtgcggc accggcacct gctcggtgag cagcatctcg tgtacgacgc agacgacatg
     7321 tgacaacact gggcagaccg gcgacacgtg cgtgttcccg gtgctgtgtc tgccgggtga
     7381 ctgcgtcggc gacccggtgt gcacgaacgc gctcacgacg gtcgactact gcacgggcac
     7441 cgcttcggcg ctgccggtgc tgtgagggag gtcgtcatgc gcagcaagca cctttgtgca
     7501 atcgcgctac tcgctgcggc atgcggcgat gatggtgcga cctcgcgcga cggtggcgtc
     7561 gacgtgcagc tcatcgacgc gccgacgtcg gttccgatga tcacgtcgtt cgtcccgtcg
     7621 ccgtcgcaga ttccggcgaa tacgccaacc caggtgacgt tcacgtggac gtacctgatg
     7681 gagccgacgc tccccgatcc gacatgctcg atcgacaacg gcatcggcgc agtcatgcgc
     7741 ggcgtgccgg tcaccgtgac gatcgctggc acgacgctgc tcacgttgac ctgcacgaac
     7801 tctgcgggcg ccggctcgaa acaggtcgtg ctcgcggtgc cgccggcggc gccgatcctc
     7861 gcgacgttca ccgcgacacc ggcgccgatc atgccgaacg ccgccacgtc ggtgacgttc
     7921 agctggacct actcgaactc gccgtcgccg gcaccgatgt gcacgatcga cggcgtcgcg
     7981 gcgacgtcgg gcaccgcgat gcagctcacg ttgtcgcaag cgcgcacgta tcggttgcgc
     8041 tgctcgaaca cgcagggctt cgcgatccgc gacgtgaccg tcccggtcaa tgaatgcgcg
     8101 ggcggcacgc acgactgcaa cgcgcacgcg acctgcaacg ataccgtcga aggcttcacg
     8161 tgctcgtgca acacgggtta cgtcggcaac ggcgatgtgt gtagccagca gaacgtgaac
     8221 tgcgtagcgg ggtgcggtgc aaatgcgacc tgcacgactg cgacgaacgc ttgcacgtgc
     8281 aacgtcgggt tcgtcggcga cggcgtgacg tgtacgcgcg agcggctgat gttcgtcacg
     8341 tcagcattcg gcacgggcaa cctgtcgtca tggtcgggcg cggtcggtac cggcctcgca
     8401 gcggctgact cgatctgcca ggccgaagcc gcgacgctag gccttactgg cacaaagttc
     8461 gtcgcgtgga tgagcgacag caacacggac gcgtactgcc acattcatga ccttacaggc
     8521 aagaaggcca ctggctgcgg cttgggcgca ggcaacttgc ccgtagccgc gggtccgtgg
     8581 gcacgggctg acgccacacg atcaccagcc gcacctgcga tcgacaagtg gcttgcgccg
     8641 atgcgccaga ccttcacgcc agtcacgttc actgccgccg gtaatgacgt ctcggcgacg
     8701 acgccgttct tcctgtacac cggtaccgac gatagcggcg tgttcaccgg cacggcgtgc
     8761 acggactgga cgacaacggc gggcacgggc gcgatgggtg acatctccgg cggcggcacg
     8821 gcgtggaccg atcagggcac ggatccgagc tgtgcgacga gcggccgcct gcgctgcgtc
     8881 gaggtcggta ccggtcctgc tctcccgtcg cgccatcccg cgaacgcgaa gcgcgggttc
     8941 gtgacgtccg tgtctggcac cggcgcgttc agtacgtggg ctgatgcgca gggctcgagc
     9001 ggcatcaccg ctgccgacga gatctgtaga tcgcgtgcgc gatttgcggg ctacgcgaat
     9061 gccgcgagct tcaaagcatg ggcctcgtac tcgacggtca gcgcaccgaa ccggttcact
     9121 tacagcgggc catggtaccg gcccgacggt atccagatcg gtacgcggac gacgctgacg
     9181 accggacgca tcctcgcgcc gatgtaccag acggaggcga acgactacgc ttcgggtaat
     9241 gccgagaccg gcagcgtgtg gaccggtaca accgcgggcg gctcgtacta caccagctcc
     9301 ggcaactgtt cgagctgggg gttcacgacg tccacggcga tcatcggcag gaccgatatg
     9361 gcggacttcc gctgggtggc ggtcggctcg agctccagcg tgccgacgta cacgaactcg
     9421 tgctcggcaa cgaactaccg tctgtactgc ctcgacgata gtccgtagag ccgagcgctc
     9481 gcgggttccg ggggcaacca cgcacctctg gacccgttcg tgcgttcgcg gggcgtatcc
     9541 gtagatgtac taaccgcttg agtagttgcc gaatccgcgg tttccttttg ggtcgatgag
     9601 cccgggcact caggttggcg cgtatcgcat cgtcaaacag atcggcgagg gcgggatggg
     9661 ttcggtctgg cttgccgagc acgcgatgct cggtcgccgc gcggccgtaa agctattgca
     9721 tccttcgtac agctcacacc aagagatcgt gcagcgcttc ttcaacgagg cgcgcgcggc
     9781 cacatcgatc tccgatccgg gaatcgtcca aatcttcgat tttggcaatc agcccgacgg
     9841 gcaggcctac atcgtcatgg agctcctcga gggcgagcca ctcgataccc gactcgagcg
     9901 ccagggcagg ctgccgatag acgagacgct tcgtattatg cgccaagtcg caagctcgct
     9961 gggcgcggcg cacgcgcgcg gcatcgtgca tcgcgacctc aagcccgaga acatcttcat
    10021 ggtgcgcgac ccggaggtcg tcggcggaga gcgtgcgaag cttctcgact tcgggatcgc
    10081 caagctcggc gagcagtcgg gcgtgcaaac gcagacgaac atgatcatgg gcacgccggc
    10141 gtacatgtcg cctgagcaat gccgcggcgc caaacacatc gatgcccgct ccgacgtcta
    10201 cgcactcggc tgcgtgatgt tcaccctgct caccggcagg ccgccgttta ccgcagaagg
    10261 gcatggcgac tggatcgtca tgcacatcac gcagcccgcg ccccggttgg cgagcgtgct
    10321 gccacacgcg tcggcgatgc tcgatcagct ggtcgccaag tgtctggaga aagatcccaa
    10381 cgcgcgcttc gcgaacggcg cagagctcgc aggcgcgctc aaccagctcg tgactcatat
    10441 gtcgcagccc ggcatgccta tcgcggcgca tgcagcggtg acgatcgcgg cgaagccgca
    10501 gactacgctg cgttcagccg ccgcgcagtc ggccgcggtg acgggaaagc aaagctcgac
    10561 gcttgcgatc gtcggtgggc tcgttgtgtt actcgcaatc ggcggcggga tcgcgttcta
    10621 cgccacgcgc gataagggcg agccagatgg acaagcgcag gcgagcgcgc cgacacctat
    10681 gccaccgaag gcagagccac cgaaaccgga gccactgccg ccaccctcgc cagcaccggc
    10741 gccacccgag ccatcgcctc cagcggaggc aaagcccgag accgtcgaca ttcgcatcga
    10801 gtcgaacccg agcggtgcag ccgtgtttgt cgcggaggag aaggtcccgc gcggcaagac
    10861 gccatacacg ctctcgatca agaagtcgga tccggcgatc gagatcaagc tcgttgcaac
    10921 cggctacgcg cccaagaagg cgaaggtgtc accagcgacg gatacgtcga tcgcggttcc
    10981 cctcacgaag gccagacgcg gtaccacggc gacgcaaacg ccgtcatcac cggtgagcga
    11041 cgacagcgac gagacaatga atccattcaa caagaagaag tgagtcgcct atgaatcgaa
    11101 ttgcgatggt ggtcgcagcg ttgatcctga gcagtgggct cgtgcttgcg cagtcgggcg
    11161 agaaagccgc cgaggaatgg ttcaacaaag gcaacgtcgc ctacgatctc ggccgttacg
    11221 acgaagccgc ggataacttt acgaaggcgt acgaggcgtg gcaacgcccg gagttcctct
    11281 acaacatcgc gcaggcctat cggctcggtg gcaactgcgc taaggcgctc cacttctaca
    11341 agcgattcaa gacgctcaag gaaaaggaca cgctcgcgcc gctccccgca aagaagcgcg
    11401 aagaagtcga tcggttcatc agcgaactca cggactgcgc agccaagggg caatcgagcg
    11461 cgaacgtccc gcccgatacc accgacagac cgccgcagtc cccgccagcg acgacgccaa
    11521 cgccaacaac gatacagacg gccgccgcgc cgccggccga gccggaggaa cccgaacagc
    11581 cggtcgacac gccggcgcgc tcgagcaacg tcgtgccgat cgccgtcggt gccggagccg
    11641 tcgtcctgtt cggcgcggcg cttggcctag agctctgggc gcgatcgaca tacagcgacg
    11701 cgaaggcgga aaacgatcca gccacgcagg agtcgctatg gcactcggcg aataccaagc
    11761 gttataccgc cgacggcctc gcggtcggcg gcgctgcttg tgcaggcatc gcggtgtggc
    11821 tgttcattcg caatcgcaac aacccgagcg agaaggaagt aacgattgca ccgatcggcg
    11881 ggccagggct tgccggcatc gggatcgcag ggagctactg atggcgaagc gaatttgtct
    11941 ggcgctgctc atcgcgtcgt gcggtgcgtt tccacaacca ccgcgcaatc agaatcccga
    12001 cgctgccccc gacaccgctg ccgccgacac gcccaacggt gcgagtttgg cagtcacgac
    12061 cctctcaccg cctgacaaca gcactggcaa tcaggcgagc actaccacgg tgacagctac
    12121 gttcgcggag actctcgact gtgcggcaat cacggatgcg agtttgaagg tgtccgagag
    12181 tggtgcggac gtcgttggtc aggtgacatg cgcggccggc acgctgacgt tccaacccac
    12241 gaacggcgta ttgcccacgg cgacgacatt gctggcgacc attgatgcga gcgtgcgtag
    12301 cgttggaggc gcgactctgg gggcaccacg ctcgtggacc ttcgacgtag cgccgtggac
    12361 gcggcagctc ggaacggcag cgctcgacgc cccttcggcg attgtttccg acgctgccgg
    12421 taacgtgtac gctgtcggct atacatcggg tgcgctcgac ggcggcacga acgtcgggct
    12481 caacgacgcg ttcatcacga agtacagcgt tacaggcgca aggcagtgga cgcgcgtatt
    12541 cggcacgacc gccaacgatc aggcgtacgg tattgcgcgt gacacggcag gagaccttgt
    12601 ggttgtcggc tcgacgaaag gtgacctcca ggccacgaat gctggtggag aagacttgtt
    12661 cgtgacgaag tacgatctcg gcgggaaccg gacttggatg cgccagctcg gcaccagcgc
    12721 ggatgacatc gctcgcggtg ttgccatcga tccaaacggc aacatctacg tcgccggtac
    12781 gacgcgcgga aacctcgatg gcacgagtgc aggtggctat gatggattcg tcgccaagta
    12841 caacggcagc ggagcactgc aatgggtccg ccagttcggc acgacagccg acgaaggtgg
    12901 gagtggtgcg atcgccgatc catcgggcaa cgtctacgtt accggctaca catcgggaag
    12961 cctcgacggt acgaacgcag gggcgacgga tatctatctg gtgaaatacg acacgaccgg
    13021 cgtcaaacag tggacacgcc aactcggaac ttcggactac gacgcaggtg acgcaatcgc
    13081 aatcgatggg atgggtaaca ttttcatcgc cggcgaaaca atgggcggcc tcgacggcaa
    13141 tacgagtgcg ggtggccagg acctcgtcct cgtcaagtac aacgacgctg gtagcaagct
    13201 ctacactagg caatttggca gcactgcgaa cgacgtaggg tatgggatcg cgatgaatct
    13261 gggtggcgag gcgtacgtga ccggaaacac agccggcgga cttgagggca atacgaacgc
    13321 tggcatgcag gacctgtacg tcgtgaagta tgcgctgaac ggcacgcggg tgtggatccg
    13381 tcaaatgggg agcgcaagct atgacgtggg gctgggcatc accgttttga gcaacgacgt
    13441 cgtcgttttc ggatctaccg gcggcggagt cgacgggaac acgaacgctg gcgccactga
    13501 ctgcatcatc gtcaagtacc aacaagacgg ccgcaaacgc tgagtgccgt ctcacgtagc
    13561 gtccgcgtgt cacacgcggg cgctacggtc aatcatgggc tgttgctcgc gcgatcagct
    13621 ctgcgatcgc tgtttggccg acgcgctcgc gcagctacgc ggcgtggcgg cctgtcgtgg
    13681 cgagtactgg gcccgctgcg tcgcggagcg ggtggtacgc acgcggctgt ggccggccta
    13741 cgacggcagg tgcaagagca tcgcgcacga caaggtccgt gatctcggcc gcgacgaacg
    13801 gctacgcgag gagctcgcgc gactctgcgc gtcgtgggcc gctcgctggt gggcgcatta
    13861 gcggaatgtc gggtcgaatt tctcgatgcg ctggagcgag aagctgtcgg accagtagac
    13921 gtaatcgccg gcgacagtga ggtagagggc ctggccctga tggtgaccgc aggcgagcat
    13981 atgaaccggg ccgccgaatc gcgacacctg cttgatgcga tacacatccg agtacgcggt
    14041 gacgaagtag aggttcttgt cgtccgcggc caggtcgaag acgagcggtt gatcctcggc
    14101 gatcgttccg actccgccgc cgtcgcgtgc caccattcgc actcg
//
LOCUS       DASUFX010000124        16306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_297988,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000124 DASUFX010000000
VERSION     DASUFX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16306
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297988"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFV99_04730"
     CDS             <1..345
                     /locus_tag="VFV99_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058640.1"
                     /translation="HEPHGHWGGWIGIGYAVPLQSTGKDPTTELKIDPQPRLDFHLGT
                     VLSLVPKWDIYADFAILDRGDRENPATMLPILDGGFDQKQIIFGITRHIEVSRKNRDY
                     DYDNDSVRLGSL"
     gene            376..1248
                     /locus_tag="VFV99_04735"
     CDS             376..1248
                     /locus_tag="VFV99_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5058641.1"
                     /translation="MANGLSVHLILILAQVCFASLPVAGRLAMQGHIGPAAIVLARMS
                     GGALVFALIAWRRGVLRIDKRDLPALFGCSILGVAANQTLFVQGLARSTATNATVLGS
                     TIPVFTALVAITLGREPVHPRRLIGIAVAFSGVAVLVGADNLSLSSGHLLGSAMILLN
                     SLCYGTYLVVVRPLAEKYDPLGLIAWLFILGVPLVLPLGLIEFARTPAFHASDIGYFA
                     FLIAVPTVSAYGLVQVALRRTESSLVAAYIYLQPVIATVGAMFLLDEQPTLRTAICGV
                     IVLFGVWLAARRAR"
     gene            complement(1202..2701)
                     /locus_tag="VFV99_04740"
     CDS             complement(1202..2701)
                     /locus_tag="VFV99_04740"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5058642.1"
                     /translation="MGVIVFAVVLSSMHYEGDVTAANGDYVDVPFDVPAGTVEIQVTH
                     TDGSDFVILDWGVWAPDGSYRGWGGGNTEDAIIGVDQSSRSYLPGPIAPGTWTVSIGK
                     AKLDATGGHYSIDVVCRDNATLQVLPKAPYSPVVLNPERRWYKGDFHVHSVQSGDAGA
                     TFDQIATLAKSRGLDFVNLSDHNTYAQHALIAAYQPSQPDLLFLRGSEITTYSGHTNS
                     VGTHDYIEHRLGYKGRAMAGIADDIAAEGAVMIVNHPVLDLGDQCIGCSWGHVDDTPW
                     EKVSAMELITGNFDIGVQAFVPRVVTLWDSLLDEGNHIAVVGGSDDHRAGMDTGPTAS
                     PIGSPTTLVLADNLSEAAIVDALHKGRTAVQLRGPDDPFVELTIGDAEIGDEISDIAD
                     VEATVHVVGGDGTIAQLWRDGKKIDQKPIAGNDTKVTFSDEPGAQLRRYRVELINDLN
                     QRIVVTSHIWVHGVAADDGCGCRSSSGSGWLGAAVIWLGALLTRRRRAR"
     gene            complement(2772..2978)
                     /locus_tag="VFV99_04745"
     CDS             complement(2772..2978)
                     /locus_tag="VFV99_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058643.1"
                     /translation="MMDELLVTCPETGRREEIGCYVARDGELLVVLRCSRFEPPAAVT
                     CATACAQCSTRSAAPVDPWRLPAD"
     gene            3436..4473
                     /locus_tag="VFV99_04750"
     CDS             3436..4473
                     /locus_tag="VFV99_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058644.1"
                     /translation="MNRDVMRASFSAVIFTLVLGVLIILGSRHLAHFDAALVGYTFAT
                     LFATFGITYRYAMWLRRPPTQMYWRRGWRTFLSPARLPGNFWRFLKRAVVEVAGNRFI
                     FRRNKLRGLAHFLLMWGCLLAAAITFPLVWGWIHFETVPGQLDTYRAYLFGFATGEFP
                     VRSVPAFIVFHGLVWASFLVLAGVMLAFRRRMRDRGAEAVQQFGEDILPLVLLAAISV
                     TGLMITISYTWLRGYAYDFLAILHAVTVIVTLLWLPFGKFFHIFQRPAQLGVGFYKDE
                     GARGEQARCKRCDRPYASAAMVRDLITVERQLGFRYEMTSGASHYQEICPACRRALFG
                     LAQGALWTETT"
     gene            4476..6743
                     /locus_tag="VFV99_04755"
     CDS             4476..6743
                     /locus_tag="VFV99_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5058645.1"
                     /translation="MSKLPVDDLAITRSFGPHLSPGTRGGTEQVPDRLVKTHCCFCGQ
                     QCGIQLKVAGNEVIGFEPWEDFPFNRGMLCPKGVKRYLQGSHPDRLLTALRRDPSAHG
                     GFSSMGYDAAIAQVATEIARLQKDHGNASVAVLGGASLTTEKTYLLGKFARVCLKTPF
                     IDYNGRLCMVSAGAANKKAFGIDRTTNPWSDMVGTDVIWVAGSNVAECSPITTNYIWQ
                     AREMGAKVIVQDPRITPVARTCDLYLPVRPGRDAALFAGVLGLMIEHGWVDRDFIAQH
                     TVGFEAVEAYCKEWPVARTAEVTGVPERSLRKAAELWGTAKTSFLFHARGIEHHSNGV
                     QNSLGTINLVLASGRIGKPKSGYGTIVGQANGQGGREHGQKCDQLPGWREIANPAHRA
                     YIADVWKIDEKELPGPGVDAYELFRKIDAGEIKGLIAICFNPKVSLPDNQFVTRALEK
                     LEFFVAIDFFLNETARHADIVLPGSLQEEDEGTVTQVEGRVIKINKAVECPGEARQDW
                     RIIQDIARALERPHGFTFDSPRAIFEELRVASKGGVADYSGITYEKIEAQMGVFWPCY
                     STDPKTGEATPDHQGTPRLFEAGSYNPVAKGAGPFYFPDGKARFNVADYRSAVDDVSA
                     DYPLFLTTGRVVSQFLSGTQTRRIGPLVKQYPEPRLELHPRLAEKLGIADGEWATCET
                     RRGAITIRAMVVTTIRPDTVFIPYHWAGDKSANRLTVAAQDPISKIPQYKVCGCRVRR
                     ADAPPSYAAEREPQQ"
     gene            6743..7393
                     /locus_tag="VFV99_04760"
     CDS             6743..7393
                     /locus_tag="VFV99_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5058646.1"
                     /translation="MPVPQHLEFFVDPSRCIGCQACVQACTECDTHKGEAMIHLEYVD
                     RAHSIQTVPVVCMHCEQPTCAEVCPADAIKRTGDGVVQSARKPRCIACGNCVVACPFG
                     VPELYIDRAIMMKCDMCYDRTSIGKKPMCATVCPSQALFFGTREQAMALRPQSMPTNK
                     FRFGEQTITTRVNVMAPRRAMPSHVDVTAAMEDKPHNRGLRLPVIDTADPFAEVEV"
     gene            7396..8052
                     /locus_tag="VFV99_04765"
     CDS             7396..8052
                     /locus_tag="VFV99_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5058647.1"
                     /translation="MPDDPQSRFEEDQQLDPRIDSPAREGLTGTARSDVYEPTRDPEE
                     VTVGPDHRPMAEQPRWRRDFATDWPEDHFVARRDFAKFLVLTSGAFVAGQVWIAANSL
                     LRKQRSAPARQKIGSLSALPPGSATMFAYPTDRDPCLLIRTRDGKLLAYSQKCTHLSC
                     AVVPKLDEGILHCPCHEGVFDLASGRNIAGPPPRPLPAIELEVVGDDIFAIGVKERTV
                     "
     gene            8049..8276
                     /locus_tag="VFV99_04770"
     CDS             8049..8276
                     /locus_tag="VFV99_04770"
                     /inference="COORDINATES: protein motif:HMM:NF042796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HEX5058648.1"
                     /translation="MTPKPQRQLTRAQRTTVVTGVLCFVLILVVLQLWLLTATMNAYL
                     GGDDGAVWPALGASVVCLMLNLGLLRYLMKR"
     gene            8273..9310
                     /locus_tag="VFV99_04775"
     CDS             8273..9310
                     /locus_tag="VFV99_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX5058649.1"
                     /translation="MTQPAPPPAGLAGMHPAYFALVMATGIESLASELMSMHSLAVGL
                     FALNLVFYPLLWILTIARIVRHRDRVRADLLHHGRAVGFFTTVAATCVLGTQCLLIGG
                     SVTAAFALWCGGIVLWALLIYGVFTLLMVKSDKPTLAEGINGGWLISVVAAQSVAVLG
                     AQLAPSFGDHAAQAALFSLAMWLGGGMLYLWIISLIFYRYMFFTLSPSDLAPPYWINM
                     GAVAISTLAGTMLVAAAPQSPAIQQILPFVRGLTLLWWATATWWIPMLVILGIWRHIV
                     RKFPLRYDPLYWGAVFPLGMYTVCTARLSKAIEAAFLVEISRGFVYVSLAAWCVATLG
                     MLLHFVRRRSA"
     gene            9696..9935
                     /locus_tag="VFV99_04780"
     CDS             9696..9935
                     /locus_tag="VFV99_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058650.1"
                     /translation="MTFSNALAVDGDTLALTGAGRLVARFGSAAGTGAIIITNGDKAI
                     AIGFLPVEATHDGDADGKSDAQELYANMIAYLCGY"
     gene            complement(9940..10584)
                     /locus_tag="VFV99_04785"
     CDS             complement(9940..10584)
                     /locus_tag="VFV99_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058651.1"
                     /translation="MQLASRIGHAAGLIERVTVRFTDINGPRGGVDTVCRIKVVMSGR
                     PSILVEKRAHAHDVAFARAVSAVGTAIERTRKKHQLHTGRRPGPSGTSRPHRPRRATR
                     DEGELIGRRVGRGPSALARALKRPEKANRAYYVDTAARGASAADRRAGGPFTARRNTL
                     ARTTRATATLEDSRTRPSRKSTRRSANRGKPSQMKERAAAAKLAMPHARASRRG"
     gene            complement(10904..12019)
                     /locus_tag="VFV99_04790"
     CDS             complement(10904..12019)
                     /locus_tag="VFV99_04790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX5058652.1"
                     /translation="MNLGDEAILEGILGELRATLPAHITVFSRNPADTLARHKVDASI
                     STRALTRREMVPEIRRLDLLILGGGGILYDRDAAEYLREVVIANELGIPVMLYAISAG
                     PLVTPSARRAVQEALNASPTTVISVRDRLGYRLLEDVGVTNEIHLTADPAFLLEPEEL
                     SVEALAAEGVDFDRHLIGFSVREPGPAAPDIRPEEYYALLANAADFMVERYDSDVVFV
                     PMEKTDVQHSHAVVAHMQNAERAEILRRRYSPRQILDLMSRFDLAVGMRLHFLIFAAL
                     RGTPFAALPYASKVTGLLEDLAMESPPLGSIGIGQLVARIDHSWDTRDAIRTKIRERV
                     PALRARAHQSNELLLELVKHREEMSAIETHQEQVTPH"
     gene            complement(12078..13646)
                     /locus_tag="VFV99_04795"
     CDS             complement(12078..13646)
                     /locus_tag="VFV99_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5058653.1"
                     /translation="MEALRTDVAIIGGGLGACAAALAVARVGRRVILTEETHWIGGQL
                     TNQAVPPDEHPWIEEMGATASYRTLRNGIRAYYRAHLPLTPQARASETLNPGNGWVSR
                     LCHDPRIAVAVLHQMMIPHQLSGRLYVMHPQRPIAAWTHGDRITGVVVRGLESGRDTL
                     IEAKFFIDATPYGDLLELAAVEHVLGAEARSETGEPHAAEQADVRDQQAITHCFAMEY
                     LPGEDHTTDKPRQYDLWRELRPPGWPGPLLSWTTVRPETLEPITRYLFEAADDHPWWR
                     FRRILDHKNFEPGFASSDITVVNWPQNDYWFGPVVGVDPAERARNLEAARELSLSLLY
                     WLQTEAPRRDGGIGYPGLRLRGDVVGGTADRLAPAPYVRESRRIRAEFTVVEQHIAHP
                     LRPDGPQLFADSVGIGCYRIDLHPRVSGAGYLDLGCWPFQIPLGAMIPLRVENLLPGG
                     KNLGVTHITNGAYRVHPVEWNVGEVAGLLAAYCLERSITPRAVRNRREELATFQDLLR
                     REGIELAWRTLKPL"
     gene            13888..14286
                     /locus_tag="VFV99_04800"
     CDS             13888..14286
                     /locus_tag="VFV99_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058654.1"
                     /translation="MLQFVQLAAGVDNVDDPIEHSIIQSIAQMVSSLAGLHIEELQPV
                     PPVGDELARLRWLQRLGAQLQSPAVRELTYALVFLTSVADLKLTSSERDALEEFQHAL
                     ALEHERVTDLVVFLTDVVATSDTSQHSATV"
     gene            complement(14328..15371)
                     /locus_tag="VFV99_04805"
     CDS             complement(14328..15371)
                     /locus_tag="VFV99_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5058655.1"
                     /translation="MSQSGRVHSIRHRSSWPGSSRGELGGTMRDACIGVDVGGSKIHV
                     GVVNREGQILATRRAPTEIERGAAALVETIATLAEQCCDEASVAPLAVGVGIAGQIDT
                     RAGVVRSSPNLPELREVALGLRLGERLQCRVIITNDLRAIARGEWRFGAGRGVSDLVV
                     LFIGTGVGGAVITGGQPLVGAGGFAGELGHMPIVANGRKCHCPGRGCVEAYVSGWALA
                     ARAAELLALHPEHTPAFSSAHGVVTAHDLAGAFAAGDMLAESIIAEAAQHLGAALVGI
                     TNVFNPARIVLGGGVLEGLPHLVHLAKKTLERDSIAVSYNRVELVHSALGSEAGVIGA
                     ATWSWDELVLENP"
     gene            complement(15304..>16306)
                     /locus_tag="VFV99_04810"
     CDS             complement(15304..>16306)
                     /locus_tag="VFV99_04810"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX5058656.1"
                     /translation="HEICIAELGMSPRTVAVLTSGGDAAGMNAAIRAVVRAALDRGWR
                     TFGVERGYAGLLDGAFRELRDRDVGGILQRGGTMLGSAPSPELHTDTGRARALGNLST
                     VADALVVIGGNGSLAGAHALARAGLTVVGIPATIDNDVHGTDFALGVDTALGVALESI
                     DRLRVTAASHRRAFVIETMGRDCGFLAATAGIAGGAEVIVIPEAPLSVDAIASEVRAA
                     YGRGKAHAIIVVAEGVRGGGAGIMQQLREQHADLGFDLRVTILGHVQRGGRPGPFDRL
                     LGSRCGIAAIDALAGGERAVMIGEQCGTLRRVPLADVAIGPRPFDPAPLELARILAR"
BASE COUNT         2722 a         5384 c         5253 g         2947 t
ORIGIN      
        1 cacgagccgc acggacactg gggcggttgg atcggtattg gctacgctgt gccgctgcaa
       61 agcaccggta aagatccgac gaccgagctg aagatcgatc cgcaaccgcg cctcgacttc
      121 cacctcggca ccgtgctgtc actcgtgccg aaatgggaca tctacgcgga cttcgcgatc
      181 ctcgatcgcg gtgatcgtga gaatccggcc acgatgctcc cgatcctcga tggcggcttc
      241 gatcagaagc aaatcatctt cggcatcacg cggcacatcg aagtcagtcg caagaatcgc
      301 gattacgact atgacaacga ctcggtgcgc ctcggcagct tgtagtccgc gccagctcag
      361 ctacgctgtg tccgtttggc taacgggctt tcagttcacc tgatcctgat cctcgctcag
      421 gtgtgcttcg cctcgctgcc ggtcgccggc aggctcgcga tgcagggcca catcggtccg
      481 gcggcgatcg tgctcgcgcg gatgtcgggc ggcgcactcg tattcgcgct gatcgcgtgg
      541 cggcgcggcg tgctgcgcat cgacaagcgc gatctaccgg cactgttcgg ctgctcgatt
      601 ctcggcgttg ctgcgaatca aacgctgttc gtacaaggcc tcgcccgttc gaccgcgaca
      661 aacgcgaccg tgctcggctc gacgatcccg gtattcacgg cgctcgttgc gatcacgctc
      721 ggtcgcgagc ccgtgcaccc gcggcgcttg atcggtattg ccgtcgcgtt cagcggcgtg
      781 gccgtgctcg tgggcgccga taacctgtcg ctgtcgtcgg gtcacctgct gggcagcgcg
      841 atgatcctgc tcaactcgct gtgctacggc acgtacctcg tcgtcgtgcg gccgctcgcc
      901 gagaagtacg atccgctcgg cctcatcgcg tggttgttca tcctcggcgt gcccttggtg
      961 ctgccgctcg gcctgatcga attcgcacgc acaccggcgt tccatgccag tgacatcggc
     1021 tacttcgcgt ttctgatcgc ggtaccgacg gtctccgcgt atggcctcgt ccaggtcgcg
     1081 ctgcgccgta ccgagtcgtc gctggtcgcc gcgtacatct atctgcagcc ggtgatcgcg
     1141 accgtcggcg cgatgttcct gctcgacgaa cagccgacgc ttcgcaccgc gatctgcggc
     1201 gttatcgtgc tcttcggcgt ctggttagca gcgcgccgag ccagataaca gccgcaccca
     1261 gccagccgct accgctgctc gagcggcagc cgcaaccgtc gtcggcggcg acgccgtgca
     1321 cccagatgtg gctcgtgacg acgatgcgct gattgagatc gttgatcagc tcgacgcgat
     1381 agcgccgcaa ctgcgcgcca ggctcgtcgg agaacgtgac cttcgtgtcg ttgccggcga
     1441 tcggcttctg atcgatcttc ttaccgtcgc gccacagctg cgcgatggtg ccgtcgccac
     1501 cgacgacgtg aacggtcgcc tcgacatccg cgatatctga aatctcgtcg ccgatttcag
     1561 cgtcgccgat cgtcagctcg acgaacggat cgtcggggcc acgcagctgg acggcggtgc
     1621 ggcccttgtg aagcgcgtcg acgatcgccg cttcgctcaa gttgtcggca agcacgagtg
     1681 tggtcgggct gccgatcggc gatgcggtcg gaccggtgtc catgccggcg cgatggtcat
     1741 cgctgccgcc gaccacggcg atgtgattgc cctcgtcgag cagcgagtcc cacagcgtga
     1801 cgacgcgcgg cacgaatgcc tgcacgccga tgtcgaagtt gccggtgatc agctccatcg
     1861 cggagacctt ctcccacggc gtgtcgtcga cgtgacccca cgagcaaccg atgcattgat
     1921 cgccgagatc gagcaccgga tggttgacga tcatcacggc gccttcggca gcgatgtcgt
     1981 cggcgatgcc ggccatggcc cggcccttgt agccaagccg atgctcgatg tagtcgtgcg
     2041 tgcccaccga gttcgtgtgg ccgctgtacg tcgtgatctc gctgccgcgc aggaacagca
     2101 gatccggctg cgacggctga tacgccgcga tcagcgcgtg ctgcgcgtac gtgttgtgat
     2161 cggacaggtt cacgaaatcg aggccgcggc tcttcgcgag cgtcgcgatc tgatcgaacg
     2221 tcgcgccggc gtcgccgctc tggaccgagt ggacgtgaaa gtcgcccttg taccagcggc
     2281 gctccgggtt cagcacgacc ggcgagtacg gcgccttcgg cagcacctgc agcgtcgcgt
     2341 tgtcgcggca cacgacgtcg atcgagtagt ggccgcccgt cgcatcgagc ttcgctttac
     2401 cgatcgacac ggtccacgtg ccgggtgcga tcgggccggg caagtagctg cgcgacgatt
     2461 ggtcgacgcc gatgatcgcg tcttcggtgt tgccgccacc ccagccgcga tagctgccgt
     2521 cgggcgccca cacgccccag tcgaggatca cgaagtcgga tccgtccgtg tgcgtgacct
     2581 ggatctcgac ggtgccggcc ggcacgtcga acggcacgtc gacgtagtcg ccgttcgcag
     2641 cggtgacgtc gccctcgtag tgcatcgacg ataggacgac agcaaagacg atcacaccca
     2701 tggctcatcg tacgctgcat ggcgtattcg tgtggagctc gaaaccggcg aagcgtgacg
     2761 cctggacgag ctcagtccgc aggtagtcgc cacggatcga ccggtgcggc gcttcgtgtc
     2821 gaacattgcg cgcatgcggt cgcgcacgtc accgccgccg gcggttcgaa tcggctgcag
     2881 cgcagcacga cgagtagctc gccatcgcga gcgacgtagc agccgatctc ctcacggcgg
     2941 ccggtctcgg ggcaggtaac gagcagctca tccatcatgc gcctcggcga ttcaacgatc
     3001 gtgccgcaat tcgtcgatcc gtcgtccagg gcaggagttg cgcgttttgt cctgaaataa
     3061 ccgtgggcat gcggcttgat aggtccacgg gccatgcgat ccatgctcat cacctgtccg
     3121 gaaacggcgg atttcgaaga gatcgagtac gtcgccagcc cgctgggcat gttgatcacc
     3181 tcgtgcacgc gattctcgcc tgcgtgcgca gttcactgtc agcgcgagtg cgcagcgcgg
     3241 ctcgaccgca agcgactgca gtcactcgtt cgtcgacgac gaagacacgc tcgtcggcga
     3301 ggacaccctt ctcaaattcg cgcagccgtt gcgtccgtga agtcgaggcc gacgtgattg
     3361 cgcgtttgtc acgggagcgt gaggtgaacg atccggaccc atgacacgtc cggttcggtg
     3421 caatatcccg ggcacatgaa ccgagacgtc atgcgcgcga gtttctcggc ggtgatcttc
     3481 acgctcgtgc tcggcgtgtt gatcattctc gggtcgcgcc atctcgctca tttcgacgcg
     3541 gcgctcgttg gctacacgtt tgcgacactg ttcgcgacgt tcggcatcac ctaccgctac
     3601 gcgatgtggc tgcgccggcc gccgacgcag atgtactggc gacgcggctg gcgtacgttc
     3661 ctgtcgccgg cgcggcttcc gggcaacttc tggcgctttc tcaagcgtgc ggtcgtcgag
     3721 gttgcaggga accgcttcat cttccgtcgt aacaagctgc gcgggctcgc gcacttcctt
     3781 ctgatgtggg ggtgtttgct cgccgctgcg atcacgttcc cgctggtgtg gggctggatc
     3841 catttcgaga cggtgcccgg tcaactcgac acctaccgcg cgtatctgtt cggcttcgcc
     3901 accggcgagt tcccggtccg ctcggtgccc gcgttcatcg tgtttcacgg gctagtgtgg
     3961 gcgtcgttcc tcgtccttgc gggcgtgatg ctcgcgttcc gccggcgcat gagggaccgc
     4021 ggcgccgagg cggtgcagca gttcggcgag gacatcctgc cgctcgtgct gctcgccgcg
     4081 atcagcgtca ccggtctgat gatcacgatc agctacacgt ggctacgtgg ctacgcgtac
     4141 gacttcctcg cgatcttgca tgcggtcacg gtgatcgtga cgctgctgtg gctgccgttc
     4201 ggaaagttct tccatatctt ccagcggccc gcgcagctcg gtgtcggctt ctacaaggac
     4261 gagggtgcgc gcggcgagca agcgcgctgc aagcgctgcg atcggccgta cgcgtccgcg
     4321 gcgatggtcc gcgacctgat caccgtcgag cgccagctcg gctttcgcta cgagatgacg
     4381 agtggcgcga gccattacca ggaaatctgt ccggcttgcc ggcgagcgct gttcgggctc
     4441 gcgcaaggcg ctctgtggac ggaaacgacg taaccatgtc caagctaccc gtcgacgatc
     4501 tcgcgatcac gcgcagcttt ggcccgcacc tgtcgcccgg cacgcgcggt ggtaccgagc
     4561 aggtgcctga ccgcctcgtc aagacgcact gctgcttttg cggccaacaa tgcgggatcc
     4621 agctcaaggt cgcgggtaac gaagtgatcg gcttcgagcc gtgggaagac tttccgttca
     4681 accgcggcat gctgtgcccg aagggcgtca aacggtacct ccagggctcg cacccggatc
     4741 ggctgctcac cgcgctgcgc cgagatccga gcgcgcatgg cgggttctcg tcgatgggct
     4801 acgacgcggc catcgcgcag gtcgcgacgg agatcgcgcg gctgcagaaa gaccacggca
     4861 acgcgtcggt cgccgtgcta ggcggcgcga gcctgacgac ggagaagacg tacttgcttg
     4921 gcaagttcgc gcgcgtctgc ttgaagacgc cgttcatcga ctacaacggc cggctctgca
     4981 tggtgagcgc gggcgccgcg aacaagaagg cgttcggcat cgatcgcacg acgaatccgt
     5041 ggtcggacat ggtcggcacc gacgtgatct gggtcgcggg atcgaacgtc gcagagtgct
     5101 cgccgatcac gacaaactac atctggcaag cgcgcgagat gggcgcgaag gtcatcgttc
     5161 aggatccgcg gatcacgccc gtcgcgcgga cgtgcgatct gtacctgccg gtgcgccccg
     5221 gtcgcgacgc cgcactgttc gccggcgtgc tcggtctcat gatcgagcac ggctgggtcg
     5281 accgcgactt catcgcgcaa cacaccgtcg gcttcgaagc ggtcgaggcg tactgcaagg
     5341 agtggccggt cgcgcgtacg gcagaagtga ccggcgtacc cgagcgctcg ctgcgcaagg
     5401 cggccgagct gtggggcacc gcgaagacga gctttttgtt ccacgcgcgc ggcatcgagc
     5461 atcactcgaa cggcgtccag aattcacttg gcacgatcaa cctcgtgctc gcctcgggcc
     5521 ggatcggaaa acctaagagc ggctatggca cgatcgtggg acaagcgaac gggcagggcg
     5581 gacgcgagca cggccaaaag tgcgaccagc tgccgggatg gcgcgagatc gcgaatcccg
     5641 cgcaccgcgc ctatatcgcg gacgtgtgga agatcgacga gaaggagctg ccgggtcccg
     5701 gcgtcgacgc gtacgagcta ttccgcaaga tcgacgctgg cgagatcaaa ggtctgatcg
     5761 cgatctgctt caacccgaag gtatcgctgc ccgacaatca gttcgtcacc cgcgcgctcg
     5821 agaaactcga gttcttcgtc gcgatcgact tcttcttgaa cgagaccgcg cggcacgccg
     5881 atatcgtgct cccgggaagc cttcaggaag aagacgaagg caccgtcacg caggtcgagg
     5941 gccgcgtcat caagatcaat aaggcggtcg agtgcccggg cgaggcgcgg caggactggc
     6001 ggatcatcca ggacatcgcg cgcgcgctcg agcgaccgca cgggttcacg tttgacagcc
     6061 cacgcgcgat cttcgaggag ctgcgcgttg cgagcaaagg tggcgtcgcc gactactcgg
     6121 gcatcacgta cgagaagatc gaggcgcaga tgggcgtgtt ctggccgtgc tacagcaccg
     6181 atccgaagac cggcgaggcg acgccggatc accagggcac gccgcggctg ttcgaggcag
     6241 ggagctacaa cccggtcgcg aaaggcgccg gaccgttcta cttccccgac ggcaaggcgc
     6301 gattcaacgt cgccgactac cggtccgcag tcgacgacgt ctccgcggac tatccgctgt
     6361 tcctcacgac gggccgcgtt gtcagccagt tcctgtctgg aacgcagacg cgccggatcg
     6421 gaccgctcgt caaacagtac cccgagccgc gtcttgagct gcacccgcgc ctcgccgaga
     6481 agctcggaat cgccgatggc gaatgggcga cgtgcgagac ccgccgcggc gcgatcacga
     6541 tccgcgcgat ggtcgtcacg acgatccgcc ccgacaccgt gttcattccg tatcactggg
     6601 caggcgacaa gagcgcgaac cggctgaccg tcgctgcaca ggatccgatc agcaagatcc
     6661 cgcagtacaa ggtgtgcggc tgccgcgtgc gtcgtgccga tgcgcctccc tcgtacgccg
     6721 ccgaacggga gccccaacag taatgcccgt cccacagcac ctcgaattct tcgtcgaccc
     6781 gagccgctgc atcggctgcc aggcatgcgt gcaagcgtgc accgaatgcg acacgcacaa
     6841 aggcgaggcg atgattcacc tcgagtacgt cgatcgcgcg cactcgatcc agacggtccc
     6901 cgtcgtgtgc atgcactgcg agcagccaac gtgcgcggag gtctgcccgg ccgacgcgat
     6961 caagcgcacc ggcgacggcg tcgtgcagag cgcgcgcaag ccgcgctgta tcgcgtgcgg
     7021 taactgcgtc gtcgcgtgtc cgttcggagt gcccgagctc tacatcgatc gcgcgatcat
     7081 gatgaagtgc gacatgtgct acgaccgcac gtcgatcggc aagaagccga tgtgcgcgac
     7141 cgtctgtccg agccaagcgc tgttcttcgg aacacgcgag caggcgatgg cgctgcggcc
     7201 ccaatcgatg ccgacgaaca agtttcgctt cggcgagcaa acgatcacga cgcgcgtgaa
     7261 cgtgatggca ccgcgccgcg ccatgccgtc gcatgtcgac gtcaccgccg cgatggagga
     7321 caagccacac aatcgcgggc tgcggcttcc cgtcatcgat accgccgatc cgttcgccga
     7381 ggtggaggtc tgatcatgcc cgacgatccg caatcgcgct tcgaggaaga ccagcagctc
     7441 gatccgcgca tcgactcgcc cgcgcgcgag ggcctcaccg gtacggcgcg cagcgatgtc
     7501 tacgagccga cgcgcgatcc cgaggaggtt accgtcggtc ccgatcaccg cccgatggcg
     7561 gagcagccgc gttggcggcg cgacttcgcg accgattggc ccgaggacca cttcgtcgcg
     7621 cggcgcgact tcgcgaagtt tctggtgctc acgagcggcg cattcgtagc cggtcaggtg
     7681 tggatcgcgg ccaatagcct gttgcgcaag cagcggtccg cgccggcgcg tcagaagatc
     7741 ggctcgctat ccgcgctgcc acccggtagc gccacgatgt tcgcgtaccc gacggaccgc
     7801 gatccatgtc tcttgatccg gacgcgcgat ggcaagctgc tcgcgtatag ccagaagtgc
     7861 acgcacctat cctgcgccgt cgtaccgaag ctcgacgaag gcatcctgca ctgtccgtgt
     7921 catgagggcg tcttcgatct cgcatccggt cgcaacatcg cagggccgcc gccacgcccg
     7981 ctccccgcga tcgagctcga ggttgtcggc gatgacatct tcgcgatcgg ggtgaaggag
     8041 cgcaccgtat gacaccaaag ccgcaacgcc agctcacgcg cgctcagcgc accacggtcg
     8101 tcaccggcgt gctgtgcttc gtcctgatcc tcgtcgtgct ccagctctgg ctgctcaccg
     8161 cgacgatgaa tgcctatctc ggaggcgacg atggcgcggt gtggccggca ctcggcgcga
     8221 gcgtcgtgtg cctaatgttg aacctcggcc tgcttcggta tttgatgaag cgatgacgca
     8281 gccggcgcca ccgcccgcgg gactcgcggg aatgcacccg gcctacttcg cgctcgtgat
     8341 ggcgaccggc atcgagtcgc tcgcgagtga gctgatgagc atgcattcgc tcgcggtcgg
     8401 tctgttcgcg ctcaatctcg tcttctatcc gctgctgtgg attctcacga tcgcgcgcat
     8461 cgtccgacac cgtgatcgcg tgcgcgccga tctcttgcac cacggccgcg ccgtcgggtt
     8521 cttcacgacc gtcgccgcaa cgtgcgtcct cggcacgcag tgcttgctga tcggaggaag
     8581 cgtaacggct gcgttcgcat tgtggtgcgg tggcatcgtg ctgtgggcgc tgctcatcta
     8641 cggcgtgttc acgctgttga tggtcaaatc cgacaaacca acgctggccg agggcatcaa
     8701 tggcggctgg ctgatcagcg ttgtcgccgc gcaatcggtg gccgtgctcg gcgcccagct
     8761 cgcaccaagc ttcggcgatc acgcagccca agcggccctg ttctcgctcg cgatgtggct
     8821 cggcggcggc atgctctatc tctggatcat ttcgctgatc ttctaccgct acatgttctt
     8881 cacgctcagc ccgtcggacc tcgcccctcc gtactggatc aacatgggcg cggtcgcgat
     8941 ctcgacactt gccggcacga tgctcgtcgc ggcggcaccg cagtcgcccg cgatccagca
     9001 gatcctaccg ttcgttcgtg gtctgacgct gttgtggtgg gcgaccgcaa cctggtggat
     9061 cccgatgctc gtcatcctcg gcatctggcg gcacatcgtt cgcaagtttc cactgcgcta
     9121 cgacccgctg tattggggcg ctgtgttccc gctcggtatg tacaccgtgt gcacggcgcg
     9181 gctctcgaag gcgatcgagg ctgcgttcct cgtcgagatc tcgcgcgggt ttgtgtacgt
     9241 ctcactcgcc gcgtggtgcg tcgccacgct cgggatgctg ctccacttcg tacgacgccg
     9301 gtcagcctga gccacgaacg cgcgtcgccg taaacctctg gcgcgctaag ggcaggctgc
     9361 gggcgagtac gcccacgtgt cgtcgagcgc gtcgacagcg tcggtggtgc cgccatgaat
     9421 gattagctgc tggcgtagcg gagcgtaagc tatcgtctgc cggttgcgcg gcgaaggccc
     9481 gctcgacgga gcttccgtcc acatcacgcc atcccactcc caggtgtcgc cgagcgatcc
     9541 gctcggcgga ttcaccgcca aacatcacga cgcgcccaag tcgtcgggtg gcccggttcg
     9601 ggggagtagc agttccatgc ggtcgtggcg gcggaaacga cggtggacgt ctcttcggca
     9661 ccctctttga tcgcgtcgaa cagctcgcgt cgccgatgac gttcagcaac gcgctcgcgg
     9721 ttgatggcga cacgctcgcg ctgaccgggg cgggccggct tgtcgcgcgg ttcggttccg
     9781 cggctggaac cggcgcgatc attatcacga atggagacaa agcaatcgcg atcgggttct
     9841 tgccggtcga ggcgacacat gacggcgacg ccgacggcaa gtccgatgcg caggagctct
     9901 acgcgaacat gatcgcgtac ctctgcgggt attgagttcc tagccgcggc gcgacgcgcg
     9961 agcatgcggc atcgcgagct tcgcggcagc cgcccgctcc ttcatctggc tcggctttcc
    10021 gcggttcgcc gagcgccgcg tgctcttgcg tgatggccgc gtgcgcgagt cctcgagcgt
    10081 cgctgtcgca cgcgtcgtac gcgcgagcgt attccggcga gccgtgaacg gaccacccgc
    10141 gcgacgatcc gctgcgctcg ccccgcgcgc ggcagtgtcg acgtagtacg cgcgattggc
    10201 tttctcggga cgtttcaacg cacgcgcgag tgcggatggt ccgcgaccga cgcgccggcc
    10261 gatcaactca ccttcgtcac gcgtggctcg acgcggccga tgtggtcgac tcgtgccgct
    10321 cggcccgggg cgacgtcccg tatgcagctg gtgtttcttc cgcgtacgtt cgatcgcagt
    10381 gccgaccgcg cttaccgcgc gcgcgaacgc gacgtcgtgc gcgtgtgctc gtttctcgac
    10441 gaggatcgaa ggtcgaccgc tcatgacaac cttgatgcgg cagacggtgt cgacaccgcc
    10501 tcgcgggccg ttgatgtcgg tgaagcggac ggtgacacgc tcgatcaatc cggccgcgtg
    10561 accgattcgc gatgcgagct gcatccggat acggtgctcg aagccacttc ctagcttgac
    10621 ggaagatcgc gtcgtcacgg gaagctcgcc gatcgagcgg cgacctgcgg accgctttcc
    10681 gattttcgag gagccctttg cgaatcgtgc gcgcgtcatg aacaaggata ccgccatgac
    10741 aaccgatggc gacgggggcg cggttgctgc cgcgtacttc gtggttacgg tcctgtcatg
    10801 agcacgatga tcatggcgat cacgttcgcc ctgctgacca tgcttgctgt tgcgtccctc
    10861 ggtcgctcgc tgaggcgatc gatggatgcg cactgagcgg tcactagtgt ggcgttacct
    10921 gctcttggtg tgtctcgatc gcactcatct cttccctgtg cttgacgagc tcgagcagta
    10981 gctcgttgct ctggtgcgct cgcgcacgta atgcgggcac acgctcacgg atcttcgtgc
    11041 gaatcgcatc gcgcgtatcc catgaatgat cgatgcgtgc gacgagctgg ccgatgccga
    11101 tgctgccgag cggcggactc tccatcgcga gatcctcgag caggccggtc actttggatg
    11161 cgtagggaag ggcggcgaac ggggtgccgc gaagcgcggc gaagatcaga aagtgcaagc
    11221 gcatgccgac ggcaagatcg aatcggctca tgagatcgag aatttgacgc ggcgaatagc
    11281 gacgtcgcag gatctcggcg cgctctgcgt tttgcatgtg cgcgacgacg gcatgactgt
    11341 gctgcacgtc ggtcttctcc atcggcacga acacgacatc ggagtcgtat cgctcgacca
    11401 tgaagtcggc cgcgttcgcg agcagcgcgt aatactcctc gggacggata tcgggggccg
    11461 ccggacctgg ctcgcgcacc gaaaatccga tcaggtgccg atcgaaatcc acgccttccg
    11521 ccgccagagc ttcgacggag agctcttctg gctcgagcaa gaatgccggg tcagcggtca
    11581 ggtgaatctc gttggtgact ccaacgtctt cgaggagccg gtagccgagg cgatctcgca
    11641 ccgagatgac cgtcgtcggc gaggcgttca gcgcctcttg caccgcacgc ctcgcggagg
    11701 gggtgaccag aggaccggcg ctgatcgcat agagcatgac cggaataccg agctcgttcg
    11761 cgatcaccac ctcgcgcagg tactccgcag catcgcggtc gtagaggatg ccgccgccgc
    11821 cgaggatcaa cagatcgagt cggcggattt ccggcaccat ctcgcgtcgc gttaacgctc
    11881 gagtcgagat cgatgcgtcg accttgtggc gcgcgagcgt atcggcgggg ttgcgcgaga
    11941 acacggtgat gtgcgcaggc agcgtcgcgc gcagctcgcc gaggatgcct tccaggattg
    12001 cctcgtcgcc gaggttcatg cctccgtacg agccggagat accaatacgc agaggttgtg
    12061 accgttccat atgcacctca cagcggtttc aatgtgcgcc acgcgagctc gatgccctcg
    12121 cgccgcagta gatcttggaa cgtcgcgagc tcctcgcgtc gattccggac ggccctcggg
    12181 gtgatgcttc gctcgaggca gtacgccgcc agcaatccag cgacttcgcc tacgttccac
    12241 tcgactggat gcacgcggta ggcgccgttc gtgatgtgcg tgacgccgag gttcttgccg
    12301 ccaggcagta agttctcgac gcggagcgga atcatcgcac cgaggggaat ctgaaacggc
    12361 cagcagccga gatcgaggta tcccgcgccg ctaacgcgag gatgcagatc gatgcggtag
    12421 caaccaatgc ccaccgaatc cgcgaatagc tgcggaccgt cgggccggag cgggtgcgcg
    12481 atgtgttgct cgacaacggt gaattccgcg cggatgcgcc gcgactcgcg gacgtacggc
    12541 gcgggcgcca ggcggtccgc cgttccaccg acgacatcgc cgcgtagccg caatccggga
    12601 tatccgatac cgccatctcg ccgcggcgct tccgtctgca accagtacag aaggctgagg
    12661 ctgagctccc gcgcggcttc gaggttgcgc gcacgttctg ccgggtcgac gccgacgacc
    12721 ggcccgaacc agtaatcgtt ctgcggccag ttgacgaccg tgatgtcgct gcttgcaaac
    12781 cctggttcga agttcttgtg atcgagaatt cggcgaaagc gccaccacgg atgatcatcc
    12841 gcggcctcga acaggtagcg cgtgatcggt tccagcgtct ccggacgcac ggtcgtccag
    12901 ctcaatagcg ggccaggcca accgggtggt cggagctcgc gccagagatc gtactggcga
    12961 ggcttgtcgg tcgtgtgatc ctctccgggc aagtactcca tcgcgaagca gtgcgtgatc
    13021 gcttgctggt cgcgcacgtc cgcttgctcg gccgcgtgag gttcgcccgt ctccgatctc
    13081 gcctcggcgc cgagcacgtg ctccacggcg gcgagctcga gcaaatcgcc gtagggcgtc
    13141 gcgtcgataa agaacttcgc ttcgatcaac gtatcgcggc ccgactcgag acctctcacg
    13201 acgacgccgg tgattcgatc accgtgcgtc cacgccgcga tcggccgctg cgggtgcatg
    13261 acgtacagac gaccgctgag ctgatgcggg atcatcatct gatggagcac tgcgaccgcg
    13321 attcgcgggt cgtgacacag acggctcacc caaccgttgc caggattcag cgtttcgctc
    13381 gctcgcgctt gcggtgtgag cggaagatgc gcgcggtagt aggcgcggat gccattgcga
    13441 agcgtgcggt agctcgcggt cgcgcccatc tcctcgatcc atggatgctc gtcgggcggg
    13501 acagcctggt tcgtgagctg gccgccgatc cagtgcgtct cctccgtcaa gatcacgcga
    13561 cggccgacac gagcaacggc gagcgcagcc gcgcacgcgc ccagtccgcc accgatgatc
    13621 gcgacatctg ttcgtagcgc ttccacggta tctcgtcgtc gagtcttcga atcgcagcgg
    13681 ttcaagaagc ggacccggaa gcggatgagt tatcaagaca gcacagcgcg cgcgactgtc
    13741 acgatgcctg gtgcagcgta cggggcggcg cttgcatggc ttcacgtggg agcttcgatg
    13801 agcatagctg tcgaccaaca acggcttcgc aggatcgtcg agacattgct accgagtggc
    13861 agactcggtc agccagaagc catcacgatg ttgcagttcg tccaactcgc cgccggtgtc
    13921 gataacgtcg acgatccgat cgagcacagc atcatacaga gcattgcgca gatggtgtcc
    13981 agcctcgcgg gactgcacat cgaggagctg cagccggttc cgccagtcgg cgacgagctc
    14041 gcacggctgc gatggttgca gcgcctcggc gcacagctac agtctccagc cgtacgtgag
    14101 cttacgtacg cgctcgtctt tctgacgagc gtcgccgatc tgaagctcac atcgtcagag
    14161 cgagacgcgc tcgaggagtt tcagcacgca cttgcgctcg agcacgagcg agtgaccgat
    14221 ctcgtcgtgt tcctgaccga cgtcgttgca acatcggaca cgtctcagca cagcgcgacc
    14281 gtgtgacgct caccgcaagc gccgcgctct tgttcagggc tcgtctgtca cggattctcg
    14341 agcacgagct catcccagga ccatgtcgcc gcaccgatca cgcccgcttc gctgccgagc
    14401 gccgaatgca ccagctcgac ccggttgtag gaaacggcga tgctgtcgcg ctctagcgtc
    14461 ttctttgcga ggtggaccag atgcggaagg ccttcgagca cgccgccacc gagcacgatc
    14521 cgcgccggat tgaacacatt ggtgataccc acgagcgccg cgcccaggtg ctgtgcggcc
    14581 tcggcgatga tcgattctgc gagcatgtct cccgcggcga acgcgccggc gagatcgtgc
    14641 gctgtcacga cgccatgtgc tgaagagaac gccggggtgt gctcggggtg aagcgcgaga
    14701 agctctgccg cgcgcgctgc caatgcccat cccgagacat acgcctcgac gcagcctcgc
    14761 cccgggcagt gacattttcg gccatttgcg acgatcggca tgtgaccgag ctcgcccgcg
    14821 aagccgccag cgccgacgag cggctgaccg cccgtgatca ccgcgccgcc cacgccagtg
    14881 ccgatgaaca gcaccacgag atccgacact ccgcggccag cgccgaaccg ccattcgccg
    14941 cgcgcgatcg ctcgcagatc gttcgtgatg atcacgcgac actgcaagcg ctcgccgagc
    15001 cgcagaccga gagccacctc ccgcagctcg ggcaggtttg gagacgaacg gaccacgcca
    15061 gctcgagtgt cgatctgtcc ggcaatcccg acacccaccg cgagtggcgc gacgctggct
    15121 tcatcacagc actgttccgc gagcgtggcg atcgtctcta cgagtgctgc agcgccgcgt
    15181 tcgatttcgg ttggtgcgcg cctggtcgct aggatctgac cttcgcgatt cacgacgccg
    15241 acgtgaatct tcgagccgcc aacgtcgacc cctatgcacg cgtcacgcat cgtccctccg
    15301 agctcaccgc gcgaggatcc tggccagctc gagcggtgcc ggatcgaatg gacgcggccc
    15361 gattgcgaca tcggccagcg gaaccctgcg cagcgtgccg cattgctcgc cgatcatcac
    15421 ggctcgttcg ccgcccgcga gcgcatcgat cgccgcaatg ccgcagcggc ttccgagcag
    15481 gcgatcgaac ggtcctgggc ggccgccgcg ctggacatgg ccgaggatcg tcacgcgtag
    15541 gtcgaatccc agatctgcgt gctgctcgcg aagctgctgc atgatgccgg cgccgccacc
    15601 tcgtacgccc tcggcgacta cgatgatcgc gtgcgctttg ccgcggccgt acgccgcacg
    15661 gacctcgctg gcaatcgcgt ccacagacag cggagcttcc ggaatgacga tcacctcggc
    15721 cccgccggca ataccggctg ttgccgccag gaacccacag tctcggccca tcgtctcgat
    15781 gacgaatgcc cgccggtgcg acgctgcggt aacgcgaagc cgatcgatcg actccagcgc
    15841 gacgccgagc gccgtgtcga caccgagcgc gaagtcagtg ccgtgcacgt cgttgtcgat
    15901 cgtcgctggg attcccacta ccgtgagtcc cgcgcgcgcg agcgcatgtg cgcctgccag
    15961 agagccgttg ccgccgatca ccacgagtgc atcggcgacc gtcgacaggt tgccaagcgc
    16021 gcgggcgcgc cccgtgtcgg tgtgaagctc cggggagggt gcgctgccca gcatcgttcc
    16081 accgcgctgc aggattccac cgacgtcgcg atcgcgcagc tcgcggaagg caccgtcgag
    16141 caggcctgca tacccacgct cgactccgaa cgtgcgccaa ccgcgatcga gcgccgcacg
    16201 cacgaccgcg cggatcgctg cgttcattcc ggccgcatct cctccgctgg tcaggacggc
    16261 gaccgttcgc ggcgacatgc cgagctcggc gatgcagatc tcgtga
//
LOCUS       DASUFX010000125         4934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_298031,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000125 DASUFX010000000
VERSION     DASUFX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298031"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2535
                     /locus_tag="VFV99_04815"
     CDS             <1..2535
                     /locus_tag="VFV99_04815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058657.1"
                     /translation="PYMVLEYLEGKTLSQVVEGKPSLHQFTEIMVSVVRALERAHEYG
                     IVHRDLKPSNIFVTDRGQVKVLDFGVARLIDRTTAATELAATRPSNAPRTEGDPRTAE
                     ATFTSNSLVGTLPYMSPEQWGIDTVDHLSDLWAVGIMFWRTFTDVHPAGSHAPDKLRA
                     VLTDLKTPLPSLGKQDPKLPAELVAIVDKCLQKQKANRYQSATELLAAMQAFLAPKQA
                     RLEADACPYRGLAAFGEDDAKYFFGRSNEIRTALGQLESWPLLAVIGPSGVGKSSFVH
                     AGLIPAMRATGTWQVCMLRPGRAPLHRLANVLDEMRATGDHLTDVLDQLTDSPGVFGK
                     LLRTSALHRSHRILVVVDQLEELFTLCDSDDARRTFLAALLAAADDPSSPVRVVLSMR
                     ADFLDRLANHKQFLSELSRGLFFLTAPDADNLRETIVRPAELAGVAFESASIVEDMML
                     AATSRGALPLVSFAATRLWDARDRERKLLTSSAYQHMGGVGGAFARHADQIAAAVPPK
                     SQPLLRAIMLRLVTPEGTRAVVDRGELVSLGDPHEVEQVLDQLVRARLIQVHVDSDQA
                     ATVEIVHEMLITEWPTLARWLTEGQALRGFMHELRQAARQWDSHGRSGDLVWRGATAQ
                     DALANAQRHVLDLSAVESAFLAAVRALAVRTRRRKVAAISTIIVMLCAVIAGGAIFTL
                     KLARANAVAQDREAAANRAAQEAQTARAALQQKLDVIEEKEQARRKAEAEAKAAQEKA
                     VKADETVKLSQEELEKANVELRRALADAESEKQRAQTEKHNAEAAAEVARKASAEARA
                     AKATAEAAAVREKARADKLEQEAKSIYNKDLRARPAAGSNQPGELK"
     gene            2532..3497
                     /locus_tag="VFV99_04820"
     CDS             2532..3497
                     /locus_tag="VFV99_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058658.1"
                     /translation="MKRVALAVLVLALVTGMPVRADDEPPWAHGVAEATQAKANELFA
                     EGNALFAQLAHAAALDKYRAAIALWDHPMIRFNMAVTLVRLDRMLEAADELDKALRFG
                     ATPFTSELYQQALDYQNLIRRQLGNIEIRCDLPGTQILLDGKPWFVAPGSQKLRVAGG
                     EHIVVAERKEYLTITRRLVVAGGSTASEQLKLVPLESAVIVEYPQPRWLPWTVTAVGA
                     AAGFGGLGLWLHAKKRLDDFDRDYTHDCATGCNMDLDKYKSLIARQDSALLEGKVGVS
                     MMVAGGAVAVGGAIWAIMNRPTRRLPKVEVAPTSGGAAATATWSF"
     gene            3518..>4934
                     /locus_tag="VFV99_04825"
     CDS             3518..>4934
                     /locus_tag="VFV99_04825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5058659.1"
                     /translation="MTLTSVLDVLRAISGYRELPELFAALVKALRPVVPADWHVMTLR
                     RGREGELFNAYSEPTIGEFSNRLRDWLPPDIMQEPHVADDARALRLYESDAVNPPILY
                     DLWQRHGVRSSLRIPLVVEGALVGAVSGWSRDPHGFDNINLDVATELGMAISVVVDSC
                     LAYEHLAQLRDEADDDHSYLLDALAMVRATEDGLIGRGAAFSTVRQQIEVVAPTDAPV
                     LLTGESGTGKDVVARAIHAASSRRDRPLVTVNCAAIPANLAESELFGHEEGAFTGATK
                     PRVGRFEQANGCTLFLDEVGELSLPVQAKLLRVLQERELERVGSTESLKIDVRIIAAT
                     NRDLPELIKADRFREDLYFRLAVFPIRLPPLRSRLEDLPALVERFVKTAAERFRVPVR
                     RVTPDAIRRLTDHDWPGNVRELQHAIERAMIISPGRELSLDAIVPRRLAPISSGSDSE
                     PLRREYLAALESTRWVIEGTDG"
BASE COUNT          864 a         1708 c         1599 g          763 t
ORIGIN      
        1 ccgtacatgg tgctcgagta cctcgaaggc aagacgttgt ctcaggtggt cgaggggaaa
       61 ccgtcgctgc accaattcac cgagatcatg gtgtcggtgg tacgtgcgct cgagcgagca
      121 cacgagtacg gcatcgttca ccgcgacctc aagccgagca acatcttcgt cacggaccgc
      181 ggtcaggtga aggtcctcga cttcggcgtc gcgcggctca tcgatcgcac gacggcggcc
      241 accgagctcg ctgcgacccg gccgtcgaac gccccgcgca ccgagggtga cccacgtact
      301 gcggaggcga cattcacgag caactcgctc gtcggcacgc tgccgtacat gtcccccgag
      361 cagtggggca tcgatacggt cgatcacctc tccgatctat gggccgtcgg catcatgttc
      421 tggcgaacgt tcacagacgt ccatccggcg ggctcgcacg cgcccgacaa gctccgcgcg
      481 gtcctcaccg atctgaagac ccctttgccg agcctcggca agcaagatcc gaagctgcca
      541 gccgagctgg tcgcgatcgt cgacaagtgc ctccagaagc aaaaggcgaa ccgctaccag
      601 agcgctaccg agctgcttgc cgcgatgcag gcgttcctcg cgccgaaaca ggcgcgcctc
      661 gaggccgatg cgtgtcccta tcgcgggctc gccgcgttcg gcgaggacga cgcgaagtac
      721 ttcttcggcc gctcgaacga gatccgcacg gcgctcggtc agctcgagag ctggccgcta
      781 ctcgcggtga tcggcccctc gggtgttggc aagtcgtcgt tcgtgcacgc cggactgatc
      841 cccgcgatgc gtgccaccgg tacctggcag gtctgcatgc tgcggcccgg acgcgcgccg
      901 ttgcaccgcc tcgcgaacgt cctcgacgag atgcgcgcga ccggcgacca cctcaccgac
      961 gtcctcgatc agctgaccga ctcgccaggc gtgtttggca agttgctccg cacctccgcg
     1021 ctacaccgca gccaccgcat tctcgtcgtc gtcgatcagc tcgaggagct gttcacgctg
     1081 tgcgactccg acgacgcgcg ccgcacgttc ctcgctgcac tgctcgcagc ggccgacgat
     1141 ccgagctcgc cggtgcgcgt cgtgctgtcg atgcgcgccg acttcctcga tcgcctcgcg
     1201 aatcacaagc agttcctcag cgagctctcg cgcggcctgt ttttcttgac cgcgcccgac
     1261 gccgacaacc tgcgcgaaac gatcgtgcgt cctgccgagc tcgcaggtgt cgcgttcgag
     1321 agcgcctcga tcgtcgaaga catgatgctg gccgcgacca gccgcggtgc gctgccgctg
     1381 gtctcgttcg cggcgacgcg actctgggat gcgcgcgatc gcgagcgcaa gctcctcacg
     1441 agcagcgcat accagcacat gggtggcgtc ggcggtgcat tcgcgcggca cgccgatcag
     1501 atcgcggctg ccgtaccgcc gaagagtcag cccctgctcc gcgccatcat gctgcggctc
     1561 gtgacgcccg aaggcacgcg tgcggtcgtc gatcgcggcg agctggtgtc gctcggcgac
     1621 ccgcacgaag tcgagcaagt actcgaccag ctcgtgcgcg cacgcctcat ccaggtgcac
     1681 gtcgattccg atcaggcggc gaccgtcgag atcgtgcacg agatgctgat caccgagtgg
     1741 ccgacgctcg cgcgctggct caccgaaggc caggcactac gcggcttcat gcacgagctc
     1801 cgccaggccg cccgccagtg ggactcgcac ggccgatccg gcgacctcgt ctggcgcggc
     1861 gcaaccgccc aggacgcgct cgcaaatgcg cagcgccacg tgctcgatct gtcggccgtg
     1921 gaatcggcat tcctcgccgc ggtacgcgcg ctcgccgtac gcacgcgacg ccgcaaggtc
     1981 gccgcgatct cgacgatcat cgtcatgctc tgtgccgtga tcgccggcgg cgcgatcttc
     2041 acgttgaagc tcgcgcgcgc gaacgctgtc gcccaggatc gcgaggcggc cgcgaaccgc
     2101 gccgcacagg aagcacagac cgcgcgcgca gctctgcaac agaagctcga cgtgatcgaa
     2161 gagaaggaac aagcgcgacg caaggccgag gccgaggcga aggccgcgca agagaaagcg
     2221 gtcaaggccg acgagaccgt gaagctctcg caggaggagc tcgagaaagc gaacgtcgag
     2281 ttgcgccgcg ccctcgccga cgccgagagc gagaagcaac gcgcgcagac cgagaagcac
     2341 aacgcagagg cggccgccga ggtcgcgcgt aaggcgagcg cagaagcacg cgcagcgaag
     2401 gcaacggccg aagccgcagc tgtccgcgag aaggcacgcg ccgacaagct cgagcaagaa
     2461 gcgaagagca tctacaacaa ggatctgcgc gcgcgcccgg ccgcgggctc gaaccaacca
     2521 ggagagctga agtgaagcgc gtcgcgctcg cggtcctcgt gctcgcgctc gtcaccggga
     2581 tgccggtacg cgccgatgac gaaccaccgt gggcgcacgg cgtcgcggag gccacgcaag
     2641 caaaggcaaa cgagctgttc gctgaaggca acgcactgtt cgcgcagctc gcgcacgcgg
     2701 ccgcgctcga caagtaccgc gccgcgatcg cgctctggga tcatccgatg atccgcttca
     2761 acatggccgt gacgctcgtc cggcttgatc gcatgctcga ggccgccgac gagctcgaca
     2821 aggcgctccg cttcggcgcg acaccgttca cgtccgagct ctatcaacag gcactcgact
     2881 atcagaacct catccgccgc cagctcggca acatcgagat ccgctgtgac cttcccggca
     2941 cgcagattct cctcgacggc aagccgtggt tcgtcgcgcc tggctctcag aagctgcgtg
     3001 tcgccggcgg cgagcacatc gtcgtcgccg aacggaagga atacctgacc atcacgcgtc
     3061 ggctcgtggt tgcgggcggc tcgacggcga gcgagcagct gaagctcgtg ccactcgaga
     3121 gcgcagtgat cgtcgagtac ccgcagccgc ggtggttgcc gtggaccgtc accgccgtcg
     3181 gtgcagccgc cgggttcggc gggctcggcc tgtggctgca cgcgaagaaa cggctcgacg
     3241 acttcgatcg cgactacacg cacgactgcg cgacgggatg caacatggat ctcgacaagt
     3301 acaagtcgct cattgccagg caagacagtg ctcttctcga aggtaaggtc ggcgtatcga
     3361 tgatggtcgc cggcggcgcg gttgccgtcg gcggtgcgat ctgggcaatc atgaaccgac
     3421 cgacccgccg gttgccgaaa gtcgaagtgg caccgacgag cggcggcgct gccgcgactg
     3481 cgacctggtc cttctgatga cgaacgacgc tacgccgatg accctcacgt ccgtgctcga
     3541 tgtcctgcgc gcgatctcgg gctaccgcga gctgcccgag ctgttcgccg cgctcgtcaa
     3601 ggcactgcgg ccggtggtcc ccgccgactg gcacgtgatg acgctccggc gcgggcgcga
     3661 aggcgagctg ttcaacgcgt actccgaacc gacgatcggt gagttctcga accggctccg
     3721 cgactggtta ccgccggaca tcatgcaaga gccgcacgtc gcagacgacg cacgcgcgct
     3781 ccggttgtac gagtcagatg cggtcaatcc accgattctg tacgacctgt ggcaacgcca
     3841 cggcgtgcgc tcctcgttgc gcattccgct cgtcgtcgag ggtgcgctgg tcggtgcggt
     3901 atccggttgg tcgcgcgacc cgcacggctt cgacaacatc aatctcgatg tcgccaccga
     3961 gctcggcatg gcgatcagcg tcgtcgtcga cagctgcctc gcgtacgagc acctggctca
     4021 gctgcgcgac gaggcggacg atgaccactc gtatctgctc gacgcactcg caatggttcg
     4081 cgcgacggag gacggcctga tcggccgggg cgccgcgttc tcgaccgtgc ggcagcagat
     4141 cgaggtggtc gcgccaaccg atgcacccgt cttgctcacc ggcgagagcg gtactggcaa
     4201 ggatgtcgtc gcgcgcgcga tccacgccgc gagcagccgc cgcgatcggc cgctggtgac
     4261 cgtcaactgt gcggccattc ccgcgaacct cgccgagtcc gagctgttcg gtcacgaaga
     4321 gggtgcgttc acgggcgcga cgaagccacg cgtcggccgc ttcgaacaag cgaacggttg
     4381 cacgctgttc ctcgacgagg tcggcgagct ttcgcttccg gtccaagcga agttgttacg
     4441 tgtgctccaa gaacgcgagc tcgagcgcgt cggcagcacc gagtcgctga agatcgacgt
     4501 gcggatcatc gcagcgacga atcgcgatct cccagagctg atcaaagcgg accgatttcg
     4561 cgaggacctg tacttccgcc tcgcggtgtt cccgatccgg ctaccgccgc tgcgttcgcg
     4621 actcgaggat ttgcccgcgc tcgtcgaacg attcgtcaag actgccgccg aacgcttccg
     4681 cgtaccggtt cggcgcgtca cgccagacgc gattcgcagg ctcaccgatc acgattggcc
     4741 cggcaacgtg cgcgagctcc agcacgcaat cgagcgcgcg atgatcatca gccccggccg
     4801 cgagctatcg ctcgacgcga tcgtgccccg ccggctcgcg ccgatcagca gtggcagcga
     4861 tagcgaaccg ctgcgccggg agtacctcgc ggcgctcgag tccacacgct gggtgatcga
     4921 aggcaccgat ggcg
//
LOCUS       DASUFX010000126        15495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1688207, whole genome shotgun sequence.
ACCESSION   DASUFX010000126 DASUFX010000000
VERSION     DASUFX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15495
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688207"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VFV99_04830"
     CDS             <1..1598
                     /locus_tag="VFV99_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5058660.1"
                     /translation="RRLLFIATLFAAACGSDVRSTPPSAADRFPRLTHAQWEATVQDL
                     FHLPAPSGLSASFTPDPQLGRFDNNIARLTTTSGLWRDYQTAAETLADRVVITPELYA
                     QLVPDPANADARELITTFGLHAFRRPLKDAEVERYLELFNSAAAVYPQYDAFKAGVRQ
                     TIAGMLQSPFFLYRPELSTTKIGIGIPLDGYEVASRLSYLFWNSMPDDELLAAAKAGK
                     LLDADGVREEANRLFDSPRAQEQFRRFHFQAFKVSQYADLDKNTTTFPLWRKEIGGMM
                     QEEALRFLDSVVTHDGGVAELLTSTKAYVNNDLARIYGVRGTFGDDYQEVDLDPNERA
                     GLLTRAGFLAKNATLTETDPIHRGVFINLDIICRQISAPPNIPTNLMKTGNTTREKVD
                     SVTGEGTCGENCHHTIINPIGFAFENYDAIGQYRLQDNGYPVNAADHYTFLDGREISY
                     TNAIDLSKQLAVSPEVHACYAQQLLEFMLGRDLQQVDKVIVSDLAKESLDDKLSIKEM
                     VLSVVSSNTFRVRAVDVQPRSQP"
     gene            1595..2977
                     /locus_tag="VFV99_04835"
     CDS             1595..2977
                     /locus_tag="VFV99_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5058661.1"
                     /translation="MITRRRFLSGVGGAAVALPFLESVRFGSRANADTPEKRVYSFFI
                     RQGNGCQQAGYNNEPERFWPQALGALTTASLAGQTDRALSVLADHAASLTLVRGTRFG
                     FPGNGCGHSGGLNQVLTAANLTGEGKDTLALGESVDWFISQRVNSAGMEPLTLMSGPQ
                     SAYIAHGLSYSGPTQLRGARNNPCAVYPTLMGLSASPEVLMQIATRRKSVNDLVREEM
                     QELLGNPKLSTTDRNRLDTHFQAIRDLEIGMAVVLDDAKVAEMKAVQTTFEANENRVK
                     VAQFMLELTALAFACDAARTGTLQIGEGNDQTRYTVDGVLQNTFHRISHRVDSDGSDG
                     TPIPNADMLHHKIDVIFAGIFKFFLDKLATYPGPSGGSLLDDSAVLWTNDLSNGPPHS
                     YSNVPQVLAGGAGGFLKTGQYIDAGNVTHNKFLNTIINAVGIRNGDGSYYDSFGDPSL
                     QKGVIAAMIK"
     gene            3046..3732
                     /locus_tag="VFV99_04840"
     CDS             3046..3732
                     /locus_tag="VFV99_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058662.1"
                     /translation="MLPHGELERLADNLYTVEGKLRMPLGETSRRMTVVRLQGNRLAI
                     YSAIALDDARMAKLESLGAPAFLIVPNALHRIDARPWQRRYPYIEVIAPHGAADKVSK
                     VVPIDANQADFGDARVTLEPVLGTDQRELAMMVETETGKTLVINDLIFNLPPRKGLMG
                     FGMRVLGFGPGHPTIPRAVKRKLIADEWEVKTQLRDWAGLDGLERIVVSHGAPIENPR
                     ETLLELAGEA"
     gene            4007..4966
                     /locus_tag="VFV99_04845"
     CDS             4007..4966
                     /locus_tag="VFV99_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5058663.1"
                     /translation="MGGPLFTFRLIPLISNELAHRGVDCADLLLEAGVPLEAQRGEVT
                     APLARIQEFLDLVSARLGDELLGLTLSRLVPPGAYAIAEFLVRAAPTVEVALRTMTDV
                     SPLINTSLRFEFEADNDEGRLHLSLVGQRDTLGRHLNEYTLAYVARQFDAMVDGGLTP
                     RSVWFSHVRDQYQQTIAETFGCEVRYGARDCGMAFPREIIARAARTSDPPLFAFLLDQ
                     ARTQLARIGSNDVVSQVMNVIDLRLARGDVGAAAVAEAMATTVRSMQRHLGEAGTTYR
                     EVLDHVRQRRLEELRRGGIAEPEIARELGFSDTKSLRRALKRA"
     gene            complement(4963..5814)
                     /locus_tag="VFV99_04850"
     CDS             complement(4963..5814)
                     /locus_tag="VFV99_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058664.1"
                     /translation="MRHLLIAVLVAACGKPGAPPPRERVLLHEDGVENAYPRLSNDGR
                     QIVYQSNRTGTWQLHILDIASRTSRALTTAANNNLPDWSADNRRIAFVSDRDGNEEIY
                     VMNRDGSESRRLTTNPGRDIHPYFSPDGATLLYNSERDGAPFDVYALDLESGRERRIT
                     NTHEDETCARYSADASQIVLLRNDARADDVWIIDAHGERNLTATPTVRDGWPTFGPHD
                     WVYFAGMDTGSFSLYRVHNDGTGREQLTSADRDEEDARPFVARDGSRVIFNRRHPGYI
                     DIVERTL"
     gene            complement(5817..8249)
                     /locus_tag="VFV99_04855"
     CDS             complement(5817..8249)
                     /locus_tag="VFV99_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058665.1"
                     /translation="MTPDAVMRELAGLHALARSLVHGDADADDLIQDTAIAAIEHPPD
                     EDRAVRPWLATVLRNRWRMDRRGRGRRQAREHEVALGDIADAEATPDAPDAIDRARTL
                     EKLASALVGLDEPFRAVVIRRYLDGQSAAEIARALNIPAGTVRWRLKTGLERLRAAMD
                     RSTPRWQRALVPFAAVKGAAVMKAKATIVSLIVLLLLVGGGIVALLVKRGDSGADKKP
                     VATVTKSAKQPGLAVTNVTGQDDAAPLRPDRLPGQGRVVVEPVAIDGGVLGGRVINWS
                     TGEGVAGAELTFTSAAGAVTVRTGKEGAFELAPPKPTMLSLTTIIAPGFLPYAPEFQH
                     SSVRIALGAKQAVRGLTLFLFPALDYYGTVVDAAGAPVAGAKVKMADPPAGEQVIEKI
                     VTEWTTDKQGTFVFHAPDFTVFEALKGSKRGWAVVDGNVATTHKMVIKIGDAAARDAT
                     IKGKTVDASGAPVADVLITAVPEDPGRKGESTRSVAFATSGPDGGFVLDGLDRQTYTL
                     VAELDEYAPVNKDKVAGGTQNVTLTLDEGQPLAGVVRDPDDKPVPAYTLLVTKREGLV
                     REVLTTRSVIDPRGHFEVRMPKGDYELLAYATGWAPSEPVKEAAGTTDAKIQLTGGAT
                     LQGVVVDADTGAPLEYARVTREGAGGGASASPANAGTVTRSDGTFELTGIPAGPFTVS
                     IGAGDYHPRLEAGLVATEGGVLGPIKVAMKKLAEGEVPKIELVGIGVKLSADGEALKV
                     EMVVPESGAAAAGITVGDHIVAIDGVPVTTLGMEGSVSRIRGVAHTKLAVSIKRGEQI
                     VPLVVERLPIRV"
     gene            complement(8334..8681)
                     /locus_tag="VFV99_04860"
     CDS             complement(8334..8681)
                     /locus_tag="VFV99_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058666.1"
                     /translation="MSGDLEAELLRVAHVLCVKRQGLREGVTASELREFIEQAPLDKT
                     FRLGHRLLSHLIEGLLEMLAAARLLERIDAMTYALTPEARAYVAREPAYLSADDWAIA
                     GTALAPDRKDRAT"
     gene            8986..10494
                     /locus_tag="VFV99_04865"
     CDS             8986..10494
                     /locus_tag="VFV99_04865"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5058667.1"
                     /translation="MTTSYITTAIPFVNARPHLGFAYELVLADILARHRRRRGHDVRF
                     LTGTDDNSLKNVVAAEREGLTTRALVDRNASAFQTLGPTLRIEADDFIRTSTDVRHRP
                     AVEHLWRACAARGDLYRRAWHGRYCPGCEAFVEDDVVVCAEHGVVPEPVSEENWFFRL
                     SRYREVVRDAIVSGRLAIVPEAARAETLAFLDGEVRDLSVSRDATRSRGWGIPVPDDP
                     TQVIYVWFDALVNYVSALGLAGDDTLLQRYWRDSERVHVIGKGISRFHAVYWPAFLLS
                     AGLPLPDRIFVHGYLTVDGEKISKSRRTVEVGPVLDDCGADALRWYFARWCPPHTDAD
                     VSIDAICAAYDRDLVDRLGNLVQRSTTLAAKLTEGRVPEPCGTPAARELEARANELPA
                     RIDAALDAFAPHEAASAAIELLDAANRYLEAAAPWRVARTNPHAAATALYAPLVAARV
                     AAAELAPFVPDVAAVLAARLGDLQPGASLIAGPPPLPRKRPRSAAPTKEQGS"
     gene            11021..13033
                     /locus_tag="VFV99_04870"
     CDS             11021..13033
                     /locus_tag="VFV99_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX5058668.1"
                     /translation="MPAADVHDKHHSEDPPPRVVILAVRPEVDGGRYPAKRVVGDTVD
                     VEVDLTSDGHDVVRAMLLHRPPGDAVWHEVELRAVGNDTWRASFVATALGRHHYTVTG
                     WVDAFATWRHGLERKVQVGTDVSVELLEGALLVDAAALRAERSGLGTAAMLRDLAGVL
                     RSAEPMESRIATALSATLADTMARAPDRTHAARYRHELELHVERPLAATSSWYELFPR
                     STGDGRHGTFRDAERWLEYVADLGFDILYLPPIHPIGLAFRKGPDNSPTSGPEDVGSP
                     WAIGAAEGGHTSVHPQLGTLDDFDHFFAAARSRGLDVALDIAFQCSPDHPWVREHPQW
                     FKQRPDGTIQYAENPPKKYQDVYPFDFECADWRNLWAALRDVFLFWCDRGVRVFRVDN
                     PHTKPIPFWEWCLREVQARVPETVFLSEAFTRPKLMYALAKSGFSQGYTYFTWRTTKW
                     ELENYLTELVHSPVADFYRPNFWPTTPDIFPEHLVYGGHAIFTQRLILAATLGANYGI
                     YGPSFELMENVQRAGVEELARNEKYQLRSWDIMRPSSLRHVIARLNRIRRAHPALHDL
                     RSLHFHRTDNELVMAYSKRRGDSIVLVVVNLDAHHTHRAWLDLDLAALGVQPEQTFQV
                     HDLLSGARYSWRGPRNFVELVPDEMPAHIFEVRRFARSENQFEYFL"
     gene            13030..>15495
                     /gene="treS"
                     /locus_tag="VFV99_04875"
     CDS             13030..>15495
                     /gene="treS"
                     /locus_tag="VFV99_04875"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX5058669.1"
                     /translation="MMTDDPLPDDPLWFKDAVIYEIHIRAFQDSTNDGIGDINGLITR
                     LDYLADLGITAIWLLPFYPSPLRDGGYDIADYTNVHPHYGQREDIAKLLREAHRRGIR
                     VVTELVLNHTSSEHPWFQRARRAPPGSPEREFYVWSDTPNRYQDTRIIFQDYELSNWS
                     WDPIANAYYWHRFYSHQPDLNFDNPAVHEALFSVIDFWFAMGVDGMRLDAVPYLYERE
                     GTSCENLPETHEFLKRLRAHIDTKFKNRMLLAEANQWPSDAAAYFGNGDECHMNFHFP
                     LMPRMFMAIELEDSFPIINILKRTPTAPPGCQWATFLRNHDELTLEMVTDEDRDTMYR
                     AYASERAARVNLGIRRRLAPLLGVRRKIELMNALLLSLPGTPVLYYGDEIGMGDNIYL
                     GDRDGVRTPMQWSSDRNAGFSRANPQKLFLPPIIDPEYHYEAVNVEAQQANPSSLLWW
                     MKRLIAKRKEHRLFGRGSLDFVTGDNPRVLAFVREYENDIVLVVANLSRYVQCSRLNL
                     ERFRGVEPTELFGRTRFPEITELPYFISLGPYDFYWLALERPQAAELASDWPTLNARG
                     SWTELLAPRQRRSLAHALIRYASQRRWFRSKARARKDVSIKDVIRLDTEGRYAIVLLH
                     VEYSHGRPETYVMPIAFAEDAAPQESLRVPSLVIANVTVDDVPGRGQVTGMLYDALIA
                     DTFGAMLLRTMTQGGGGTGERGALTGTALSALRDLPPDTPLPPRLTSVEQTNSSIVFG
                     DRLMLKLFRVIEEGPSLEYEVGHFLATREPPYRGVPRLAGALEYKAPDIDASTLGTLF
                     EFVPNQGDAWRFTLDALDRYFDHV"
BASE COUNT         2647 a         5276 c         4873 g         2699 t
ORIGIN      
        1 tgcgacgtct attgttcatc gccacgttgt ttgccgccgc ctgcggcagc gacgtccgca
       61 gcacgccgcc gagcgctgcg gatcgattcc cgcggctgac ccatgcgcaa tgggaggcga
      121 cggtccagga cctgttccat ctcccagcac cgtcgggctt gtcggcgtcg ttcacgcccg
      181 atccgcagct cggccggttc gacaacaaca tcgcgcgact caccacaacg tccggcttgt
      241 ggcgcgacta tcaaaccgcg gccgagacgc tcgccgatcg cgtcgtcatc acgccggagc
      301 tctacgcgca gctcgtgccc gaccctgcga acgcagacgc gcgcgagctc atcaccacgt
      361 tcggtctcca tgcatttcgt cgtccgctga aggacgccga ggtcgagcgc tacctcgagc
      421 tgttcaacag cgcggccgcg gtgtacccgc aatacgacgc gttcaaagcc ggcgtgcgcc
      481 agacgattgc cggcatgctg cagtcgccgt tcttcctgta tcgccccgag ctgtcgacga
      541 cgaagatcgg aatcggcatc cctctcgatg gctacgaggt cgcctcgcgg ctgtcctatc
      601 tgttctggaa ctcgatgccc gacgacgagc tactcgcagc cgcgaaggcc gggaagctcc
      661 tcgatgccga cggcgtgcgt gaggaagcga accggctgtt cgacagtccg cgtgcgcagg
      721 agcagttccg ccgcttccac ttccaggcgt tcaaggtgtc gcagtacgcc gacctcgaca
      781 agaacacgac cacgttccca ctctggcgca aggagatcgg cggcatgatg caggaggagg
      841 cactccgctt cctcgatagc gtcgtgacgc acgatggcgg tgtcgccgag ctgctcacct
      901 cgacgaaggc gtacgtcaac aacgacctgg cgaggatcta cggcgtgcgc ggtacgttcg
      961 gtgacgacta ccaggaagtc gatctcgatc cgaacgagcg cgcgggtctg ctcacacgcg
     1021 cgggcttcct cgcgaagaac gcgacgctga ccgagaccga tccgattcat cgcggcgtgt
     1081 tcatcaacct cgacatcatc tgccggcaga tctcggcacc gccgaacatc ccgacgaacc
     1141 tgatgaagac cggcaacacg acgcgcgaga aggtcgacag cgtcaccggc gaaggcacct
     1201 gcggcgagaa ctgccaccac acgatcatca atccgatcgg gttcgcgttc gagaactacg
     1261 acgcgatcgg ccagtatcgc ctgcaggaca acggctaccc ggtcaacgcc gccgatcact
     1321 acacgttcct cgacggccgc gagatctcct acacgaacgc gatcgacctc tcgaagcaac
     1381 tcgccgtgtc accggaagtc cacgcctgct acgcgcagca gttgctcgag ttcatgctcg
     1441 gccgcgatct gcagcaggtc gacaaggtga tcgtgagcga cctcgcgaag gagtcgctcg
     1501 acgacaagct ctctatcaag gagatggtgt tgtccgtcgt atccagcaac acgttccgcg
     1561 tccgcgcggt cgacgtccaa ccccggagcc agccatgatc acccgacgcc gattcctcag
     1621 tggtgttggc ggcgccgccg tggcgctgcc gttcctcgag agcgtgcgct ttggatctcg
     1681 cgccaacgcg gatacgccgg agaagcgcgt ctactcgttc ttcatccgtc agggcaacgg
     1741 ttgccagcag gcgggctaca acaacgagcc cgagcggttc tggccgcaag cgctcggtgc
     1801 gctgacaacc gccagcctcg caggccagac tgatcgcgcg ttgtcggtgc tggcggacca
     1861 cgcggcgtca ctcacgctcg tgcgcggcac gcgattcggg ttccccggca acggttgcgg
     1921 ccactccggt ggcttgaacc aggtgctgac ggccgcgaac ctcaccggcg agggcaagga
     1981 cacgctcgcg ctcggcgagt cggtcgactg gttcatctcg cagcgcgtga attcggcggg
     2041 catggagccg ctcacgctga tgtccgggcc gcagtcggcg tacatcgcgc acggcctctc
     2101 gtacagcggg ccgacgcagc tgcgcggcgc gcgcaacaac ccgtgtgcgg tctacccgac
     2161 gctgatgggt ctatctgcct cgcccgaagt gctgatgcag atcgcgacgc gtcgcaagag
     2221 cgtcaacgat ctcgtgcgcg aggaaatgca ggagctcctc ggcaacccga agctgtcgac
     2281 taccgatcgc aaccgcctcg acacgcactt ccaggcgatt cgcgatctcg agatcggcat
     2341 ggcggtcgtg ctcgacgacg cgaaggtcgc cgagatgaaa gcggtccaga cgacgttcga
     2401 agcgaacgag aaccgcgtca aggtcgcgca gttcatgctc gagctcaccg cgctcgcatt
     2461 cgcttgcgac gccgcccgca cgggaacgct gcagatcggc gagggcaacg accagacgcg
     2521 ctacaccgtc gacggcgtgc tccagaacac gttccatcgc atctcgcatc gcgtcgacag
     2581 cgatggcagc gatggcacgc cgatcccgaa cgcggacatg ctgcaccaca agatcgacgt
     2641 gatcttcgcc ggcatcttca agttcttcct cgacaagctc gcgacgtatc cgggcccgag
     2701 cggcggctcg ttgctcgatg attcggccgt gctgtggacg aacgatctgt ccaacggtcc
     2761 gccgcactcg tactcgaacg ttccgcaggt gctcgccggt ggtgcgggtg gcttcttgaa
     2821 gacaggccag tacatcgatg ccggcaacgt cacccacaac aagttcctga acacgatcat
     2881 caacgcggtc gggatccgga acggtgacgg gagctactac gacagcttcg gcgatccatc
     2941 actccagaag ggtgtcatcg ctgcgatgat caagtagggc gcggttcttg cgttgcttcc
     3001 tctcatagca gggaggagca cgtgaaaccg cacgatactt gggaggtgct gccgcacggc
     3061 gagctcgagc gcctggcaga caacctctac accgtcgaag gaaaacttcg gatgccgctc
     3121 ggcgagactt cgcggcggat gaccgtggtg cgcctacaag gtaaccgact cgcgatctac
     3181 agcgcgatcg cactcgatga cgcgcgaatg gcgaagctcg agtcgctcgg cgcacccgcg
     3241 tttctgatcg tgccaaacgc tctccaccgc atcgacgcgc ggccgtggca gcggcgttat
     3301 ccgtacatcg aagtcatcgc gccgcacggc gcggccgaca aggtcagcaa ggtcgttccc
     3361 atcgatgcaa atcaggcgga cttcggtgac gcgcgcgtca cgctcgagcc tgtgctcggc
     3421 accgatcaac gcgagctcgc gatgatggtc gagaccgaga ccggcaagac gctcgtgatc
     3481 aacgatctga tcttcaatct gcctccgcgg aagggcctca tgggtttcgg catgcgcgtg
     3541 cttgggttcg gacctggcca tccgacgatc ccgcgagccg tgaagcgcaa gctgatcgcc
     3601 gacgagtggg aagtgaaaac gcagctccgc gactgggccg gtctcgacgg gctcgagcgc
     3661 atcgtggtgt cgcacggtgc accgatcgag aacccacgcg agacgttgtt agagctggcc
     3721 ggggaagcct agctcgtcga ggaatcgtag ccgcgaggcg ttcagaagcg taaaccgcga
     3781 gagacaggga gcgcggtcac cggtgtctac gcgagtccgc ggctcgtcgg agattggtaa
     3841 cggcgcagac gagtgatttt gcatacgcag aaagcgcacc ggcgggaccg ctatccaccg
     3901 gaagctggag ccgccaggtg tcaccagcta acgccgcgag aatgcgtgac tgggcctcgg
     3961 aacacctctt gatagggtcg ccggacatca cttcgagcgt ggtgtcatgg gtggtcccct
     4021 tttcacattc cgactgatcc cgttgatcag caacgagctc gctcaccgcg gcgtcgactg
     4081 cgccgacctg ctcctcgaag caggcgtccc actcgaggcc cagcgcgggg aagttactgc
     4141 gccgctcgcg cgtatccagg agttcctcga cctcgtgagc gcgcgcctcg gcgacgagct
     4201 gctcggcctt acgctctcgc gcctcgtccc gccgggtgcg tacgcgatcg cggagttctt
     4261 ggttcgcgcc gcgcctacgg tcgaggtcgc gcttcgcacg atgaccgacg tctcgccgct
     4321 catcaatacg tcgctacgct tcgagttcga agcagacaac gacgaggggc gcctgcacct
     4381 ctctctcgtc ggccagcgcg acacgctcgg ccgccacctc aacgagtaca cgctcgcgta
     4441 cgtcgcgcgg cagttcgacg cgatggtcga tggcggcctg acgccgcgaa gcgtctggtt
     4501 ctcccatgtc cgcgatcagt accagcagac gatcgccgag acgttcggtt gcgaggtgcg
     4561 ctacggcgcg cgcgattgcg gcatggcgtt cccgcgcgag atcatcgcgc gcgcagctcg
     4621 gacatcggac ccaccgctgt tcgcgttcct gctcgatcag gcgcgcacgc agctcgcccg
     4681 catcggttcg aacgacgtcg tctcgcaagt gatgaacgtg atcgacctgc gccttgcccg
     4741 tggtgatgtc ggcgcggccg cggttgccga agcaatggca acgaccgtgc gcagcatgca
     4801 gcgccacctc ggcgaggcgg gcacgaccta tcgcgaggtg ctcgatcacg tacggcaacg
     4861 ccggctcgaa gagctgcgcc gcggcggcat cgccgagccc gaaatcgcac gcgagcttgg
     4921 cttctccgac acgaagtcgc tgcgtcgcgc actcaagcgc gcttagagcg tgcgctcgac
     4981 gatatcgata taacccgggt gacgccggtt gaagatcacg cgcgagccgt cgcgcgcgac
     5041 gaacggtcgc gcatcttcct cgtcgcgatc ggcactcgtc agctgctcgc gacccgtgcc
     5101 gtcgttatgc acgcggtaca aactgaagct gccggtgtcc atccccgcga agtacaccca
     5161 gtcgtgcggg ccgaacgtcg gccagccatc gcgcacggtt ggcgtcgcgg tcagattgcg
     5221 ttcgccgtgc gcgtcgatga tccagacatc gtcggcgcgc gcgtcattgc gtagcaggac
     5281 gatctggctc gcgtcggccg agtaacgcgc acacgtctcg tcctcgtgcg tgttggtgat
     5341 gcgccgctcg cggccactct cgagatcgag tgcgtagacg tcgaacggcg cgccgtcgcg
     5401 ctcggagttg tagagcagtg tcgccccgtc gggactgaag tacggatgga tgtcgcggcc
     5461 cggattcgtc gtgagccggc gcgactcgct accatcgcgg ttcatcacgt agatctcctc
     5521 gttgccgtcg cgatcgctga cgaacgcaat gcgacgattg tcagcgctcc agtcgggcag
     5581 gttgttgttc gcggccgtcg tgagcgcgcg ggaggtgcgg ctggcgatgt cgaggatgtg
     5641 cagctgccac gtgccggtgc gattcgactg atagacgatc tggcgaccat cgttcgacag
     5701 ccgcggatac gcgttctcga cgccgtcttc gtggagcagc acgcgctcgc gaggcggcgg
     5761 tgcgcccggt tttccgcagg ccgctaccaa gaccgcgatc agcagatggc gcatcactac
     5821 acccgaatcg gcagccgttc gacgacgagc ggcacgatct gctcgccgcg cttgatcgag
     5881 actgcgagct tcgtgtgcgc gacgccgcga atgcgcgaca cggagccttc catcccgagc
     5941 gtcgtcaccg gcacgccatc gatcgcgacg atgtggtcgc ccaccgtgat gcctgccgcc
     6001 gcggccccgc tctcgggcac gaccatctcg accttcagtg cctcgccatc tgccgacagc
     6061 ttcacgccga ttccgacgag ctcgatcttc ggcacctcgc cctcggcgag cttcttcatc
     6121 gcgaccttga tcggaccgag cacaccgccc tcggtcgcga cgagcccagc ttcgagccgc
     6181 ggatgatagt cgcctgcgcc gatcgacacc gtgaacggtc cggcgggaat cccggtgagc
     6241 tcgaacgtgc cgtcgctccg cgtcaccgtg cccgcattcg ccggcgacgc actcgcacca
     6301 ccgcctgcgc cttcgcgcgt gacccgcgca tactcgagcg gcgctccggt atccgcgtcg
     6361 acgacgacgc cctgcagcgt cgcgccgccg gtcagctgga tcttcgcgtc ggtcgtgccc
     6421 gccgcttcct tgaccggctc actcggcgcc cagccggtag cgtacgcgag tagctcgtag
     6481 tcgcctttcg gcatccgcac ctcgaagtgt ccgcgcggat cgatcaccga acgcgttgtc
     6541 aggacctcgc gtaccaaccc ctcgcgcttc gtcaccagca acgtgtacgc gggcacgggc
     6601 ttgtcgtcgg gatcccggac cacgcctgcc agcggctgac cttcgtcgag ggtcagcgtg
     6661 acgttctgcg tgccgccagc aaccttgtct ttgttcaccg gcgcgtactc gtcgagctcg
     6721 gcgacgagcg tgtacgtctg ccgatcgagc ccgtccagca cgaacccgcc gtcgggacca
     6781 ctcgtcgcga atgcgaccga gcgcgtcgat tcgcccttcc tccccggatc ttcgggcacc
     6841 gccgtgatca gcacgtcggc aaccggcgcg ccgctcgcat ctaccgtctt gcccttgatc
     6901 gttgcgtcgc gcgctgccgc gtcgccgatc ttgatcacca ttttgtgtgt cgtcgcaaca
     6961 ttgccgtcga cgaccgccca accgcgcttg ctccctttca acgcctcgaa caccgtgaag
     7021 tcgggcgcgt gaaacacaaa cgttccctgt ttgtcagtcg tccactcggt cacgatcttc
     7081 tcgatcacct gctcgccggc cggcggatcc gccatcttca ccttcgcgcc cgccaccggc
     7141 gcacctgccg catcgacaac cgtcccgtag taatcgagcg cgggaaacag aaacagcgtc
     7201 agcccccgca ccgcttgctt cgccccgagt gcgatccgca ccgacgagtg ctggaactct
     7261 ggcgcatacg gcagaaaccc cggcgcaatg atcgtcgtca gcgacaacat cgtcggcttc
     7321 ggcggcgcga gctcgaacgc accctccttg cccgtgcgta cggtgaccgc gcccgccgct
     7381 gacgtgaacg tcagctccgc accggccacg ccctcgcctg tcgaccagtt gatgacgcgg
     7441 cccccgagca caccgccgtc gatcgcaacc ggctcgacga cgacccggcc ctgccctggc
     7501 aagcgatccg gccgcaacgg tgccgcgtcg tcctgacccg tcacattcgt cacggcaagc
     7561 ccaggttgct tggcgctctt cgtgaccgtc gccaccggct tcttgtcggc accgctgtcg
     7621 ccgcgtttga cgagcagcgc gacgatgccg ccgccgacga gcagcaacaa cacgatgaga
     7681 gaaacgatcg tggccttggc tttcatgact gcagctcctt tgaccgcagc aaacggcacg
     7741 agtgcgcgct gccatcgcgg tgtcgagcga tccattgcgg cacgcagccg ctcgagtccg
     7801 gtctttagac gccagcgcac ggtgccggcg ggtatgttca acgcacgcgc gatctcggcg
     7861 gcggattggc cgtcgagata gcggcggatc acgacggcgc gaaacggttc gtccaacccg
     7921 accaaggccg acgccagctt ctcgagcgtg cgcgcgcgat cgatcgcatc gggagcatcg
     7981 ggcgtggcct cggcatccgc gatgtcgcca agcgcgacct cgtgctcgcg cgcctggcgt
     8041 cgtccgcgtc cgcgtcgatc catgcgccag cgattgcgca gcacggttgc gagccacggc
     8101 cgtaccgcac gatcctcgtc gggcggatgc tcgatcgcgg cgatcgcggt gtcctggatc
     8161 aggtcatcgg cgtcggcgtc gccgtgcacg agagaacgcg ccagcgcatg caggccggcg
     8221 agctcgcgca tgaccgcgtc gggcgtcacc tccagagcat gccgcgaacg cgcatagtgt
     8281 tggcggtgat cgcctgcgac gcctgcgacg cctgcggcat tctcggatcc gcgtcatgtg
     8341 gctcggtcct ttcggtcggg tgcgagtgca gtgccggcaa tcgcccagtc gtccgcggac
     8401 aaataggcag gctcgcgtgc gacgtaggcg cgagcctccg gcgtcaacgc gtaggtcatc
     8461 gcatcgatgc gttcgagaag cctggcggct gccagcatct cgagcagtcc ctcgatcagg
     8521 tgcgatagca aacggtggcc gagccgaaac gtcttgtcga gcggagcttg ctcgatgaat
     8581 tcccgcagct ctgaagccgt gactccttcg cgtaggccct ggcgctttac gcacaggacg
     8641 tgcgcgacgc gcagtagctc ggcttcgagg tcacccgaca tgccgcgacg acacgagcaa
     8701 ctgcgatgcc gacgccggcg cctgtcactt cacgacactt gcgtcgcgct cgacggtatc
     8761 ggcgcagaca aagtgacacg gctaccgaga acgcgatggc gttctgacgt ggtcaggcta
     8821 taacagtcac gtgaaccgcg cgtttcgaat gtcgatgccc atgtgctctg cgaagagctt
     8881 cgggcatccg tgcgcgcact gatctaatcg cgccgagcgg ctgccctcgt ggcagctgca
     8941 ccggcttcgg cgagggcggc ctccggcgtc acctggagga acgccatgac cacgtcttac
     9001 atcacgactg ccattccctt cgtcaacgct cgcccgcacc tcggcttcgc gtacgagctc
     9061 gtgctcgccg acatcctcgc gcgccaccgg cggcgccgcg gccacgacgt ccgcttcctg
     9121 accggtaccg atgacaacag cttgaagaac gtcgtcgccg ccgagcgcga agggctcacg
     9181 acgcgcgcgc tcgtcgatcg caacgcgtct gcgtttcaga cgctcgggcc cacgctgcgc
     9241 atcgaagcgg acgactttat ccgcacgtcg accgatgtac ggcatcggcc tgcggtcgag
     9301 catctttggc gcgcgtgcgc ggcgcgcggc gatctgtacc gtcgggcgtg gcatggccgc
     9361 tactgccccg gatgcgaggc gttcgtcgag gatgacgtcg tcgtgtgtgc ggagcatggt
     9421 gtcgtgcccg agccggtcag cgaagagaac tggttctttc gactgtcgcg ctatcgcgag
     9481 gtcgtgcgcg acgcgatcgt ctcgggccga ctcgcgatcg tgccggaggc ggcacgcgcc
     9541 gagacgctcg cgttcctcga cggtgaggtg cgtgatctga gtgtgtcgcg tgacgcgaca
     9601 cgatcgcgcg gctgggggat tccggtgccg gatgatccga cgcaggtcat ctacgtctgg
     9661 ttcgacgcgc tggtgaacta cgtgtcggcg ctcgggctcg ccggtgacga cacgctgctc
     9721 cagcggtact ggcgtgacag cgagcgcgtg cacgtgatcg gtaaaggcat ctcgcggttc
     9781 cacgccgtgt actggcccgc cttcttgctg tcggcaggcc tgccgttgcc ggatcggatc
     9841 tttgtccacg gttacctcac ggtcgacggc gagaagatca gcaagtcgcg ccgaaccgtc
     9901 gaagtcgggc cggtactcga cgactgcggg gcggacgcac tgcgctggta tttcgcgcgc
     9961 tggtgcccgc ctcacaccga tgccgacgtt tcgatcgatg cgatctgcgc tgcgtacgat
    10021 cgcgacctcg tcgatcggtt gggcaatctc gttcagcgca gcacgacgct ggcggccaag
    10081 ttgaccgaag gccgcgtacc ggagccgtgc ggtacgccgg ctgcgcgcga gctcgaagcg
    10141 cgagcgaatg agcttccggc acgcatcgac gcagctctcg atgcgtttgc acctcacgaa
    10201 gcggcaagtg cggcgataga actgctcgac gctgcgaatc gctacctcga agccgccgcg
    10261 ccctggcgag tcgcgcgcac gaatccacac gctgccgcga ctgcgctgta tgcaccgctc
    10321 gtagcagcac gcgtggccgc agccgagctc gcgccgttcg tgcccgatgt cgctgccgtg
    10381 ctcgcggcac ggcttggcga tctgcagcct ggcgcatcac tgatcgccgg accaccgccg
    10441 ttaccccgca agcgtcctcg tagcgccgca ccgacgaagg agcaaggctc ctgaagaatc
    10501 gatccgccga gcaactctcg tgctcgctgc tcgatcacct atcggcgtgg tttgcggccg
    10561 accattggcg cgtgcgctag tcaacgcggc cccagcgctg cggtgggcgt ccccgtgtca
    10621 cctacgcgac caacagagat ggcctcgact ggcgctggca cattcgctgc tctcttcggg
    10681 tggaaccacg aggacctgga gcaccgaaat gagattcgcg cgtcgttcgt tcttttttcg
    10741 ctggcgagac gacaccgcgc ccagtcagcc gtcggcgccg ctagccgacg cggtcccagc
    10801 agagcggcgc gacgcgcacg ccgtcggtgt tcggcttgac ggcaacgatg taccacctgc
    10861 catcgacggc gatgatctcg ggtgacgaga cacgcacctc gccaccttac gtcggcatcc
    10921 ggatcgcgag cgagcgccag agcacaggtt aggtgcgagc taggtcgagg gcgcgcggct
    10981 cccgatcgtt gcgaaagcgc tagccttcta tgagcgcggc atgccggcgg ctgatgtaca
    11041 cgacaagcat cacagcgagg acccaccacc ccgcgtcgtg atcttggcgg tacgacctga
    11101 agtcgatggc gggcgctacc cggcaaaacg cgtcgttggt gacaccgtcg atgttgaagt
    11161 cgatctcacg agcgacggcc acgacgtcgt acgcgcgatg ctactgcacc gtccgcccgg
    11221 cgatgccgta tggcatgaag tcgagctacg cgcggtcggc aatgacacct ggcgcgcgag
    11281 cttcgtcgcg accgcgctcg gccgccatca ctacacggtg accggatggg tcgatgcgtt
    11341 cgccacgtgg cgtcacggcc tcgagcgcaa ggtgcaggtc ggcaccgatg tcagcgtcga
    11401 gctactcgag ggcgcgctgc tcgtcgatgc ggcggcactg cgcgccgagc gatcgggcct
    11461 cggtacggcc gcgatgctgc gcgatctcgc cggcgtattg cgcagcgccg agccgatgga
    11521 atcgcgcatc gcgaccgcgc tcagcgcgac actcgccgac accatggcgc gagctcccga
    11581 tcgcacgcat gcggcgcgct atcggcacga gctcgagctc catgtcgagc gaccgcttgc
    11641 cgcgacgtca tcgtggtacg agctgtttcc ccgctcgacc ggcgacggcc gacacggcac
    11701 gtttcgcgac gccgaacgct ggctcgagta cgtcgccgac ctcggcttcg acatcctgta
    11761 cttgccgccg atccatccca tcggtctcgc gttccgcaaa ggtccggaca actcgccgac
    11821 ctccggcccg gaagacgtcg gcagcccgtg ggcgatcggc gccgccgagg gcgggcacac
    11881 gagcgtgcat ccgcagctcg gcactctcga cgatttcgat cacttcttcg cagcggcacg
    11941 ctcgcgcggc ctcgacgtcg cactcgacat cgcgttccag tgctcgcccg atcatccgtg
    12001 ggtgcgcgag catccgcagt ggttcaagca acgcccagac ggcacgatcc agtacgccga
    12061 gaatccgccc aagaagtacc aggacgtcta cccgttcgac ttcgagtgcg cggactggcg
    12121 taacctgtgg gcggcgctgc gtgacgtgtt cttgttctgg tgcgatcgtg gcgtccgcgt
    12181 gtttcgcgtc gacaatcccc acacgaaacc gattccgttc tgggagtggt gccttcgcga
    12241 agtccaggca cgcgtgcccg agaccgtgtt tctgtccgaa gcgttcacgc gaccgaagct
    12301 gatgtacgca ctcgcgaaga gcggcttctc gcagggctac acgtacttca cgtggcgcac
    12361 gacgaagtgg gagctcgaga attatttgac cgagctcgta cattcgccgg tcgcggattt
    12421 ctatcgaccg aacttctggc cgacgacgcc cgacatcttt cccgagcacc tcgtgtatgg
    12481 cggccacgcg atcttcacgc agcggctgat cctcgccgcg accctcggcg cgaactacgg
    12541 catctacggc ccgtcgttcg agctgatgga gaacgtgcag cgcgccggcg tcgaggagct
    12601 cgcgcgcaac gagaaatacc agctgcgcag ctgggacatc atgcgaccga gctcgttgcg
    12661 ccacgtcatc gcgcggctca atcggatccg gcgcgcacac cctgcgttgc acgacctgcg
    12721 ctcgctgcac tttcaccgca cagataatga gctcgtgatg gcgtactcga agcgccgcgg
    12781 agatagcatc gtgctcgtcg tggtgaatct cgacgcgcat cacacccacc gcgcatggct
    12841 ggatctcgac ctggcggcgc tgggcgtgca gcccgagcag acgttccagg tgcacgacct
    12901 gttatctggc gcccgttatt cgtggcgagg tccgcgtaac ttcgtcgagc tggtccccga
    12961 cgagatgccg gcgcatattt ttgaggtgcg ccgcttcgcg cgcagcgaga atcagttcga
    13021 gtacttccta tgatgacgga cgacccgcta cccgacgatc cgttgtggtt caaagacgcc
    13081 gtcatctacg agatccacat ccgcgcgttc caggactcga cgaacgacgg catcggcgac
    13141 atcaacggtc tgatcacgcg gctcgattat ctggcggacc tcggcatcac cgcgatctgg
    13201 ctcttgccgt tctatccgtc gccgctgcgc gacggtggct acgacatcgc ggactacacg
    13261 aacgtgcacc cgcactacgg ccagcgcgaa gatatcgcga agctgttgcg cgaggcgcat
    13321 cgccgcggca ttcgcgtggt caccgagctc gtgctcaatc acacttcgtc ggagcatccg
    13381 tggtttcagc gcgcgcggcg cgcaccgccc ggcagccccg agcgcgagtt ctacgtgtgg
    13441 agcgacacgc cgaatcgcta ccaggatacg cgcatcatct ttcaggacta cgagctgtcg
    13501 aactggtcgt gggacccgat cgcgaatgcg tactactggc accggttcta ttcgcaccaa
    13561 ccggatctca acttcgataa tcccgcggtt cacgaagcgc tgttctcggt gatcgatttc
    13621 tggtttgcga tgggcgtcga tggcatgcga ctcgacgccg tgccgtacct ctacgagcgc
    13681 gagggcacga gctgcgagaa cctgcccgag acccacgagt tcttgaagcg gctgcgcgcg
    13741 cacatcgaca ccaagttcaa gaaccgcatg ctgctcgcag aagcgaacca gtggccatcc
    13801 gacgccgccg cgtattttgg caacggcgac gagtgccaca tgaactttca cttcccgctc
    13861 atgccgcgca tgttcatggc gatcgagctg gaggacagct tcccgatcat caacatcctc
    13921 aagcgcactc cgacggcacc gcccggctgt cagtgggcga cgttcttgcg caaccacgac
    13981 gagctcacgc tcgagatggt gactgacgaa gatcgcgaca cgatgtaccg cgcgtacgca
    14041 tcggaacgcg ccgcgcgtgt gaacctcggc attcgccgcc ggctcgcgcc gctgctcggt
    14101 gtgcgacgca agatcgagct gatgaacgcg ctgctgttgt cgctgccggg gacgccggtc
    14161 ctctactacg gcgacgagat cggcatgggc gacaacatct acctcggtga tcgcgatggc
    14221 gtgcgcacgc cgatgcagtg gagctcggac cgcaacgccg gcttctcgcg cgcgaacccg
    14281 cagaagctgt tcctgccacc gatcatcgac cccgagtatc actacgaggc ggtcaacgtc
    14341 gaagcgcagc aagcgaaccc gtcgtcgctg ctgtggtgga tgaagcggct gatcgcgaag
    14401 cgcaaagagc atcgcttgtt cggccgtggc tccctcgact tcgtgaccgg cgacaacccg
    14461 cgcgtgctcg cattcgtccg cgagtacgaa aatgatatcg tgctcgtcgt cgcaaatctg
    14521 tcgcgttacg tgcagtgttc tcgtctcaac ctcgagcgct tccgtggcgt cgaacccacg
    14581 gagctgttcg gccgcacgcg attccccgag atcaccgagc tgccttactt catctcgctc
    14641 gggccatacg acttctactg gctcgcgctc gaacgcccgc aagctgccga gctggcatcc
    14701 gactggccga cgctgaacgc gcgcggctcg tggacagagc tgctggcccc gcggcaacgc
    14761 aggtcgctcg cgcacgccct gatccgttac gcgtcgcagc gtcgttggtt ccgcagcaag
    14821 gcgcgtgctc gcaaagacgt ctcgatcaag gacgtgatcc ggctcgacac ggaaggccgc
    14881 tacgcgatcg tgctgctcca cgtcgagtac tcgcacggcc gtccggagac atacgtgatg
    14941 ccgatcgcgt tcgccgagga tgcggcgccg caggaatcac tgcgcgtgcc gtcgctcgtg
    15001 atcgcgaatg tcaccgtcga cgatgttccc ggccgcggtc aggtgaccgg tatgctgtac
    15061 gacgcgctca tcgcggatac gtttggtgcg atgctgctgc gaacgatgac gcagggaggc
    15121 ggcggtacgg gcgaacgcgg cgcgctcacg ggcacggcgc tgagtgcgct ccgcgacctg
    15181 ccgcccgaca cgccgctgcc gccgcgattg acctcggtcg agcagacgaa cagcagcatc
    15241 gtgttcggcg atcgtctgat gctgaagctc ttccgcgtga tcgaagaggg tccgagcctc
    15301 gagtacgagg tcggccactt cttggcaacg cgcgaaccac cgtatcgcgg cgtgccacgc
    15361 ctcgcaggcg cgctcgagta caaggcgccc gatatcgatg cgagcacgct cggcacgctg
    15421 ttcgagttcg tgccgaacca aggcgatgcc tggcgcttca cactcgatgc actcgatcgc
    15481 tatttcgacc acgta
//
LOCUS       DASUFX010000127        15722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1966597, whole genome shotgun sequence.
ACCESSION   DASUFX010000127 DASUFX010000000
VERSION     DASUFX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15722
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966597"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFV99_04880"
     CDS             <1..905
                     /locus_tag="VFV99_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5058670.1"
                     /translation="LPRQGLGNAAVAAVASPEQKKAFAKRWIAMAITEPSAGSDSAAI
                     RTTAKRDGDHWILDGEKIFVTAGERADAVVVWASLDLSLGRAAIKPFLVPKDAPGIRV
                     ARLEHKLGIRASDTATIVLESCRIPAENILGSPEIDTSKSFAGVMKTFDNTRPVIAAM
                     AVGVARAALERTRELLAASDPSIRDPRPGPRASHAAHVLFGLEAELEAARLLALRAAW
                     MADRGIPNSLEASMAKAKAGRTVTEITLRCVELCGATGYAEGELLEKWARDAKILDIF
                     EGTQQIQLLIVARRMLGKSSAELR"
     gene            905..1120
                     /locus_tag="VFV99_04885"
     CDS             905..1120
                     /locus_tag="VFV99_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058671.1"
                     /translation="MQELLIEAQSELGLADPAPLATLTPAQLAWLRDAARDARKREEA
                     SLDAAVDRALEHVPWLLRGAVKRILFP"
     gene            1117..2139
                     /locus_tag="VFV99_04890"
     CDS             1117..2139
                     /locus_tag="VFV99_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058672.1"
                     /translation="MSDRRGELVKLARVLDLPPDVLAHLALDADSLRSVREALSASLF
                     DASRPTMQRVAKASKLLPNGTVARVGEHVFGPLLCARIVGLLAPEHAIDLALRMPDEF
                     LADTSALVDPRQAQTLIAAMPTERVVEVAGRLVERGDYVTLARFVDFLSRETIEAVIA
                     SIRDELVLLHIAAFVESQKKLTELVGLIPRTRLRAMIVAVGATGGPLWLEALALTEHL
                     DQHWKQTIGDLAAELDAAVLQSMLYTARDYAAWDSVLPLIVAMSEWAQQRFIELPGID
                     DVVLVGLLGAADRLGLWQELLPIVPKMPLRLREMLATLLGGLPTDVIDRILTAADPHP
                     WLTAAS"
     gene            2161..2667
                     /locus_tag="VFV99_04895"
     CDS             2161..2667
                     /locus_tag="VFV99_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058673.1"
                     /translation="MFVTGTSFGQVLAQTIQWCAPRARLDDPAGSLRSERLRPWPLAR
                     DRIAVVSMLATMRTNQLLGSPHVADARSADDLHGGRLLVHFPGDTLFEEACFDSSRGF
                     FDKYDAPPWDTWITVRADGKREYLVSWVPPMFFELAEIGIKVSSTSCVRWLEDAPEAD
                     ELWRFRVR"
     gene            complement(2664..3578)
                     /locus_tag="VFV99_04900"
     CDS             complement(2664..3578)
                     /locus_tag="VFV99_04900"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5058674.1"
                     /translation="MDTLETMTYEVTGKIARITLNRPKRGNGITLALPQELAACVERA
                     NLDPKVHVIALSGNGTGFCGGYDLVESAEGMAMGVEEAKRQKDTPLDPAVIATNHMPT
                     NVWDPVTDYQMMSRNVRGFMSLFYGDKPVVCKVHGFCVAGGTDMALCSDLLVIADDAK
                     IGYPPARVWGSPTTSLWFYRLGLEKAKRLLFTGDCLSGKEAKEWGLAIESAPPDKLNE
                     RFEILLERIARMPINQLVMMKLLLNQTLLAQGLHTTQLVGTVFDGITRHTKEGYGFQQ
                     RAMEKGFREAVRERDEPFGDFGLSTFKG"
     gene            complement(3627..4442)
                     /locus_tag="VFV99_04905"
     CDS             complement(3627..4442)
                     /locus_tag="VFV99_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX5058675.1"
                     /translation="MKAITIGFVPLVLALATSTAHADRALTYERLYFRAGLLHISPLS
                     DSRELVLEGVHGPASLALMDGPIAGSGAALEPMTIPAVIAGYTLPVLHDRLSLETVLS
                     TPLHVRFRATGSLADMSIAPEALGISTGVPALGSELGEADAAPPIVTAVYRVADLGPV
                     HPYGGLGVGVLVTYNAHATNPVLTEVDDPTFHIDPAPGLVLQAGTDVTLWRRVSFRVD
                     VKYIAFMKAHATVENLQVRTPEIPIFETAKVGSATMEMWVNPLIVQLGLGVDF"
     gene            complement(4439..7327)
                     /locus_tag="VFV99_04910"
     CDS             complement(4439..7327)
                     /locus_tag="VFV99_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5058676.1"
                     /translation="MRDRRPPLLFACCLAGCYSAGPVDEPPPPSVVFTYPVDNQLDVP
                     LGARVLVSYSDAVADQAGCTLAGPDGNVEITKQVVGHGKTVAITSSALLPLTTYQVTC
                     GGSGELAGEPLFRFTTRNDRPLAGAPSLIAVNGSALATLDALHPGAFRPIFESSTLQL
                     VFSEPLDPRSVTTDNGAVELVDANGAPVPATLLANGIHVTLDPAAPLAAGATYQLRLG
                     NQLADVGGERLVPTAIAFTPLDSLGRGPIRQVFRTWQSGDPVASVARTDDMNVMAVSH
                     PLIGEANAAMKPNVLETELGDPLALDGPIAFRIPKGQRLTSAGLAIQLAGAIPSGLET
                     GDIAIEMLTDGGGRLYRNRYQPAGTLPDNTTSPLLVDLSFDLAIYATDPTGNAVLAQT
                     VLGIQLTGLAIADEGALAIETLGALDINLLGMSSAPTNIVLDLISDLKASPAPDVQPP
                     TLLSSLPAADSHDLITDEGIELVFDEPVDIDRARAGAITLRDGNTVVPSSLEIHGSVV
                     VVRPRATLLGNHAYQVDLSEVGDLAGNVMPLQTFAIATQLVAQTDVPPSVTAVYPGAP
                     CSLVDVKTNTAGRCSGGASADDRYRPFALAANERIAVVFDQPLYGASLTLGDACNTGS
                     IRVERVDAMGTCLEAVPGTLVKHQRDIGFIPDGGWRPGEHYQLRLVSGPNATCDVGEV
                     CGANGRPANFDPLGGTTASASGGPDLLVSFVGTDPTKATTLLATASPNTDLNGSGRME
                     QGEQLREDNRVALRIAGATGLISSASFQGPDCMPSTPEVESCMYVAGAIPAQLGERRE
                     NCILPGGTMVPTCIPVAMSAQAMYSTSVTMTAGALGILPITTETGMSVMRVRENPDGP
                     LEGYIVDRNGTPTMIAALGLYMDAPDMSLPIAQHDMHSKPLWVTLEGPLQFKPDGRIA
                     ISLRNIADVPISVGIDAPLGIKGAVQLIVPAGEMRLQLMSRAQRGSLP"
     gene            complement(7488..8177)
                     /locus_tag="VFV99_04915"
     CDS             complement(7488..8177)
                     /locus_tag="VFV99_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5058677.1"
                     /translation="MAAGKAKKVKTTKPKASAPRRATGVQLGSDVARNMIMFGATRVF
                     AMKGFRGVSVEDLLEASQVSRRTFYRFFKSKEDVALAMYTLGTSSLLEACRRAITNEK
                     DLITQLERCIDIHLGNARSMGRLVYVLGGEAQSLESPLHARRMEVHDLLIAMFHDASD
                     ESKRVDPLLIRTLVFALEQIVRTVLEQGDEGRRVTNESILRARTVMMRVATGTVAGHG
                     DRVAPLPMTLA"
     gene            complement(8298..9257)
                     /locus_tag="VFV99_04920"
     CDS             complement(8298..9257)
                     /locus_tag="VFV99_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058678.1"
                     /translation="MRRLRSADWPHAMVVASATASSIACGRFGFVDAPDGAIDVPNVI
                     DASTCFGKPFGAAQPVAMIHQAGYDDWGGSPTANGSELYFHSFRPAALAGANIFVAKG
                     TAPNSYGIPELVMELSTGGAEFQPTVTADGLDVIFAADMGLPYLLELYESQRASTTTA
                     WSPPARIAELGTDAQHQLDPWLTADGLALYYGIGPDLNNAKLYRATRASRQSMWNGPV
                     LVPGIQGAGPFAVSPTLSPDEREIWFATDLDSPGNLDIVTARRPDRDSPFGALERVMP
                     LNTPADDAALHLSVDGRYLYLVENTDYVVGGNATISVATRICE"
     gene            9280..10335
                     /locus_tag="VFV99_04925"
     CDS             9280..10335
                     /locus_tag="VFV99_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEX5058679.1"
                     /translation="MTRWVASLLVVVFVGCGGSTGGGGGGDGGGGGDDMPIDAPLGTW
                     TYVPIDGMVCADGSPTGIGVNLSDTSSDVFIFVAGGGACWDATTCFTTKSAVHIEGGY
                     GQADFNTEINGLAGAGIFQRIAQNPFKDASWVYVPYCTGDLHDGANVATYDAAHVVHH
                     VGRTNAAALLARVAATRPAAQTVWLVGVSAGGYGIAFDWDLARAEWPQSKVHALADSS
                     PLVAMEPTRWSAMQASWKLTFPTACASCTSDLGAMNAALRSTQRPGERYALLANTRDQ
                     TISTYFGVTMDQLQTETLAAQAAMSPSMGQAAYVIAGTGHVLLGNPSVQTSNGVVLAT
                     WFAQWASGDPAWANAGP"
     gene            10373..11323
                     /locus_tag="VFV99_04930"
     CDS             10373..11323
                     /locus_tag="VFV99_04930"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEX5058680.1"
                     /translation="MSTGAADILTREEIRSFTQRSNFVGAIAIAWSWLVIIGCFVAVA
                     LVPHPAVFVGAVILLGGRQLALAVAMHEAAHGTLFKSRWANEVLTDWLCARPVWSDTA
                     RYRVHHLQHHAHTGTDRDPDLGLALAEPMSRRSLRRKLLRDIFGASGVRRVVGLLAID
                     AELLEFDVGGSPRRAKFRSRGYHLRALAKNIWKPILANLVMFGALYAAGVAWVYLAWV
                     VAYLTMFSLVIRVRSLAEHACTARTDDQLLNTRTTRANLAARMTLAPLHVNYHLEHHL
                     LPTVPWWRLPALHRALVERGALPSQSLARGYLHVLQLVSR"
     gene            11331..12143
                     /locus_tag="VFV99_04935"
     CDS             11331..12143
                     /locus_tag="VFV99_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058681.1"
                     /translation="MRARLAVGVVALIGCGRLGFDSAPGPRDAPASSDVDSPPAYQAS
                     SVRFDPAGGDYMWTNMLENTPNSGRGTFSAWFHFNGGDGQQQLITVAQTIGFGGVLRT
                     TTNKFHFILPACTGITVLDMQTQNAYTSTSGWIHVLAAWDVTTGKADLYVNDIPDRAA
                     NPLIVNGSICYAALRWGIGGYTGNGLDADVADFYSALGTYIDLSVEANRRKFIDNTGR
                     PVDLGPACNGPTGATSTGCFTGDPATWNMNKGVAAGFTLEGDGLAPAASPSD"
     gene            complement(12140..>15722)
                     /locus_tag="VFV99_04940"
     CDS             complement(12140..>15722)
                     /locus_tag="VFV99_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058682.1"
                     /translation="QLSSFADEVTRVARDVGVEGKLGGQAAVRGVAGTWKELTDNVNQ
                     MASNLTNQVRDIAYVTTAVANGDLTRKITVEVRGELLQLKNTINTMVDQLGSFASEVT
                     RVAREVGIDGVLGGQAEVRGVSGTWRELTDNVNVMARNLTEQVRGIANVVTAVANGDL
                     DRKLNLAARGEIATLVDTINNMIDTLSTFAAQVSGVARDVGVDGRLGGQADVPGAAGV
                     WRDLTNNVNELAGNLTNQVRAIRDVATAVTQGDLTRSITVEARGEIAQLKDTVNQMIR
                     TLAETTKVNQEQDWLKTNVARFTRMLQGQRDLLTVARQILNELAPLVNAHHGAFYMAE
                     ADDEGQILRLFASYAFQERKAVANVWRFGQGLVGQAALEGKRIVVGKVPADYVQITSA
                     LGEAPPHSIVVVPILFEGEVKGVIELASFEKFSGIQLAFLDQMLESLGIVIATIEATM
                     RTDELLRQSQSLTEELRSQQDELQQTNEELEEKAHQLTEQKAEVERKNRQVELARQEL
                     EEKAEQLALTSKYKSQFLANMSHELRTPLNSLLILSRQLADNAEQNLTDKQIRYAETI
                     RQAGTDLMTLINEILDLAKIESGTMAVEIGPVRCANLRDYVDQTFRQVAEEKGLQFSV
                     ELDEHLPGSIETDDMRLRQVLRNLLSNAIKFTERGRVKLRITKTSNDVIAFSVADTGI
                     GIPADKQRLIFEPFTQADGSTSRKYGGTGLGLAISREIAGLLGGELRVDSVIGQGSTF
                     TLYLPTVYKQRDPSVRRVPQPVPQLPQVIDLTQPESVDKEEAPPPPPLTRTVPDDYES
                     LEPGDRVLLIVEDDVTFAMTMLEMARASSFKGVVAMTGHQALELARSIKPDAITLDLR
                     LPDLDGWVLLDRLKHDAATRHIPVHIVSGLDEERRGLQSGAHGFLQKPVTEEDLKAGL
                     DKVRGFVEKRVKQLLVVEDDPVQSQAISDLIGDGDVETTTVASGEIALQTLAEKPYDC
                     VVLDLRLPGMSGFDLIEQIKADPQHRRLPVIVYTGRDLTDEDRQRLHGLAQTVIIKDV
                     TTMERLLDETALFLHRVEANLPERKQRVLRRLAKSDPQLAERTVLVVDDDLRNIFALT
                     SLLEGHKMNVLYAENGKRALAKLEENPSIDVVLMDIMMPEMDGYEAVRRIRERSEWKD
                     LPVIALTAKAMKGDREKCLEAGASDYITKPVDADQLLSLLRVWLYQS"
BASE COUNT         2484 a         5159 c         5289 g         2790 t
ORIGIN      
        1 cgttgccgcg gcagggtctc ggtaacgccg ccgtcgccgc cgtggccagc cccgagcaga
       61 agaaggcgtt cgcaaagcgc tggatcgcga tggcgatcac cgaaccgtcc gccggttcgg
      121 attccgcggc gattcgcacc accgcaaagc gcgacggcga tcactggatt ctcgacggcg
      181 agaagatctt cgtgaccgcc ggcgagcgcg cggatgccgt cgtcgtgtgg gcgtcgctcg
      241 atctgtcgct cggccgcgcc gcgatcaagc cgttcctcgt accgaaggat gctcccggca
      301 ttcgcgtcgc acggctcgag cacaagctcg gcattcgcgc gtcggacacc gcgacgatcg
      361 tcctcgagag ctgccgcatc cctgccgaga acatcctcgg cagccccgag atcgatacga
      421 gcaagtcgtt cgcgggcgtg atgaagacgt tcgacaacac gcgacccgtg atcgccgcga
      481 tggcggttgg cgtcgcgcgc gcggcactcg aacgcacgcg cgagctgctt gcggcgagcg
      541 atccgtccat tcgcgatccg cggcccggtc cgcgcgcgtc gcacgccgcg cacgtgctgt
      601 tcgggctcga agccgagctc gaagccgcgc gccttctcgc actacgtgcc gcctggatgg
      661 cggatcgtgg cataccgaat tcgctcgaag cctcgatggc caaggcgaag gcgggccgca
      721 ccgtcaccga gatcacgctt cgttgcgtcg agctgtgcgg agccaccggt tacgccgagg
      781 gcgagctgct cgagaagtgg gcacgcgacg ccaagatcct cgacatcttc gagggcacgc
      841 agcagatcca gctgctgatc gtcgcccgca gaatgctggg caagtccagc gcggagcttc
      901 gctgatgcaa gagctattga tcgaagccca atccgagctc ggcctcgctg accccgcgcc
      961 actggccacg ctcacgcctg cgcaactcgc ctggttgcgc gacgctgcgc gagatgcgcg
     1021 caaacgcgaa gaggcatcgc tcgacgccgc cgtcgatcgc gcgctcgagc acgtgccgtg
     1081 gctgttgcgt ggcgcggtga agaggatcct gttcccgtga gcgaccgccg cggagagctc
     1141 gtcaagctcg cacgcgtgct cgacctgccg ccggacgtcc tcgcgcatct cgcgctcgac
     1201 gccgatagcc tgcgcagcgt gcgcgaggca ttgtcggcat cgctgttcga cgcgtcgcgg
     1261 ccgacgatgc aacgagttgc gaaggcgtcg aagctcctgc cgaacggcac ggtcgcccgt
     1321 gtcggcgagc acgtgttcgg gccgctgctc tgcgcgcgca tcgtcggcct gctcgcgccg
     1381 gagcacgcga tcgatctcgc gctgcggatg cccgatgagt ttctcgccga tacctccgcg
     1441 ttggtcgatc ctcggcaagc gcagacgctg atcgcggcga tgccgaccga gcgtgttgtc
     1501 gaggtcgctg gccggctcgt cgagcgcggc gactacgtca cgctcgcccg cttcgtcgac
     1561 ttcctgtcac gcgagacgat cgaagccgtg atcgcaagca tccgcgacga gctcgtgctg
     1621 ctgcacatcg cagcatttgt cgagtcgcag aagaagctga ccgagctcgt gggcctgatt
     1681 ccccgcacac ggctacgcgc gatgatcgtc gcggtggggg caacaggcgg tccgctgtgg
     1741 ctcgaagcgc tcgcgctcac cgagcatctc gatcagcact ggaagcaaac gatcggcgac
     1801 ctcgcggccg agctcgatgc ggccgtgctc cagtcgatgc tgtacaccgc gcgcgactac
     1861 gcggcgtggg acagcgtgct gccgctgatc gtcgcgatga gcgagtgggc gcagcagcgc
     1921 ttcatcgagc tacctggcat cgacgacgtc gtgctcgtcg gcctgctcgg tgccgctgac
     1981 cgcctcgggt tatggcaaga gctactcccg atcgtgccga agatgccgct gcgtctgcgc
     2041 gagatgctcg cgacgctact cggtggctta cccaccgacg tcatcgatcg aatcctgacc
     2101 gccgccgacc cacatccgtg gctgactgca gcgagctaac ccagctgcta cgctagaggt
     2161 gtgttcgtca ccggcacgtc gttcgggcaa gtgctcgcgc agacgatcca gtggtgcgcg
     2221 ccacgtgctc ggctcgacga tccggcgggc tcgctgcgca gtgaacggct caggccatgg
     2281 ccgctggccc gtgatcgaat cgcggtcgtg agcatgctcg cgacgatgcg gacgaaccag
     2341 ctgctcggct cgccacatgt cgccgatgcg cgctcggcag acgacctcca tggcgggcgc
     2401 ctgctcgtcc acttccccgg cgacacgctg ttcgaagaag cgtgcttcga tagctcgcgc
     2461 ggcttcttcg acaagtacga cgcgccgccc tgggacacgt ggatcacggt ccgcgccgac
     2521 ggcaaacgtg agtacctcgt ctcgtgggtg ccgccgatgt tcttcgagct cgccgagatc
     2581 ggaatcaagg tgagctcgac gtcctgcgtg cgctggctcg aggacgcgcc cgaggccgac
     2641 gagctctggc gatttcgcgt tcgctagcct ttgaacgtcg acaggccgaa gtccccgaat
     2701 ggttcgtcgc gctcgcgcac ggcttcgcgg aagcccttct ccatcgcgcg ttgctggaag
     2761 ccgtagcctt ccttcgtatg gcgcgtgatg ccgtcgaaca cggtgccgac gagctgcgtc
     2821 gtgtgcaggc cctgcgcgag cagcgtctgg ttgagcaaca gcttcatcat gacgagctga
     2881 ttgatcggca ttcgcgcgat gcgttcgagc aggatctcga agcgctcgtt cagcttgtcg
     2941 ggtggcgccg attcgatcgc gagcccccac tccttcgctt ccttgccgga tagacagtcg
     3001 cccgtgaaca gcagccgctt cgccttctcg aggccgagcc ggtagaacca cagcgacgtc
     3061 gtcggcgaac cccagacgcg tgccggcggg taaccgatct tcgcgtcgtc ggcgatgacg
     3121 agcaggtccg agcacagtgc catgtcggtg ccgcccgcga cgcagaagcc gtgcacctta
     3181 cacacgacgg gcttgtcgcc gtagaacagg ctcatgaagc cgcgaacgtt gcggctcatc
     3241 atctgatagt cggtcaccgg gtcccacacg ttcgtcggca tgtggttcgt cgcgatgacc
     3301 gctggatcga gcggcgtgtc tttctggcgc ttcgcctcct cgacgcccat cgccatgcct
     3361 tcggcgctct cgaccagatc gtagccgccg cagaatccgg tgccgttgcc gctgagcgcg
     3421 atgacatgca ccttcggatc gaggttggcg cgctcgacgc acgcggcgag ctcttgtggc
     3481 agcgccagtg tgatgccgtt accgcgcttc ggccgattga gcgtgatgcg cgcgatcttg
     3541 cccgtgacct cgtaggtcat tgtctcgaga gtgtccactt catgcctcca gcaggtgcga
     3601 tgcgcgcagc atcgctcaaa gcgtcgctag aagtcgacgc cgagacccag ctggacgatc
     3661 agcggattga cccacatctc catcgtcgcg ctcccgacct ttgcggtctc gaagatcggg
     3721 atttcgggcg tgcgaacctg cagattctcg acggtggcgt gcgctttcat gaacgcgatg
     3781 tacttgacgt cgacgcggaa gctgacgcgt cgccacagcg tgacgtcggt accggcctgc
     3841 agcacgagcc cgggcgccgg gtcgatgtga aacgtcgggt cgtcgacttc ggtcaggacc
     3901 gggttcgtcg cgtgggcgtt gtacgtgacg agcacgccaa cgcccaggcc tccgtacgga
     3961 tgcacggggc cgagatcggc aacgcgatag acggccgtga cgatcggcgg tgcggcgtcg
     4021 gcttcgccca gctccgagcc gagcgccggc acaccggtcg agatgccgag cgcctcgggc
     4081 gcgatcgaca tgtctgcgag cgagccggtc gcgcggaagc gaacgtggag cggcgtgctg
     4141 agcacggtct cgagcgagag ccgatcgtga agcaccggca gcgtgtagcc ggcgatcacc
     4201 gccgggatgg tcatcggctc gagcgctgcg ccggagcccg cgatcgggcc atccatcagc
     4261 gcgaggctcg ccgggccgtg cacgccttcg agcacgagct cgcgcgaatc ggagagcggc
     4321 gagatgtgga gcaggcccgc gcggaagtag aggcgctcgt aggtgagcgc gcggtcggcg
     4381 tgcgcggtcg acgtggcgag ggcgaggacg aggggaacga agccgatcgt gatcgctttc
     4441 atggcaggct cccccgttgc gcgcgggaca tcagttgcag ccgcatctcg cccgctggga
     4501 cgatgagctg gacagcgcct ttgatgccga gcggtgcgtc gatcccgaca gagatcggaa
     4561 catcggcgat gttgcggagc gagatcgcga tacgcccatc cggtttgaac tgcagcggcc
     4621 cctcgagcgt cacccatagc ggcttcgagt gcatgtcgtg ctgcgcgatc ggcaagctca
     4681 tgtcgggcgc gtccatgtac aggccgagcg cggcgatcat cgttggcgtg ccgttgcgat
     4741 cgacgatgta gccctcgagc ggcccgtcgg ggttctcgcg cacgcgcatc acgctcatgc
     4801 cggtttcggt cgtgatcggc aggatgccga gcgcgccggc cgtcatcgtc accgacgtcg
     4861 agtacattgc ctgggcgctc atcgcgacgg gaatgcaggt cggaaccatc gttccaccgg
     4921 gaaggatgca gttctcgcgg cgttcgccga gctgcgccgg aatcgcgccg gcgacgtaca
     4981 tgcacgactc gacttcgggc gtgctcggca tgcagtccgg accctggaac gacgccgacg
     5041 agatcaggcc ggtcgcgccg gcgatgcgca gcgcgacgcg attgtcctcg cgcagctgct
     5101 cgccttgctc catgcggccg gagccgttga ggtcggtgtt cgggctcgcc gtagccagca
     5161 gcgtcgttgc cttcgtcggg tcggtgccga cgaagctcac gagcagatcc ggtccgccgc
     5221 tcgcgctcgc ggtcgtgccg ccgagcggat cgaagttcgc cggccgacca ttcgcgccac
     5281 agacctcgcc gacgtcgcac gtcgcgttcg ggcccgacac gagccgcagt tggtagtgct
     5341 cgcctggccg ccagccaccg tcggggataa agccgatgtc gcgctggtgc ttcacgagcg
     5401 taccaggcac ggcttcgagg cacgtgccca tcgcatcgac gcgctcgaca cgaatgctcc
     5461 ccgtgttgca ggcgtcgccg agtgtgagtg acgccccgta cagcggctga tcgaacacga
     5521 ccgcgatgcg ttcgtttgca gctagcgcga acgggcgata gcgatcgtcg gcactcgcgc
     5581 cgccggaaca gcgcccggcg gtgttcgtct tgacgtcgac gagcgagcac ggcgcacctg
     5641 gatagaccgc agtcaccgac ggcggtacat cggtctgcgc gaccagctgc gtcgcaatcg
     5701 cgaacgtctg cagcggcatc acgttgccgg ctaggtcgcc cacttcgctg aggtcgacct
     5761 gatacgcgtg attgccgagc aaggtcgcgc gcggccgcac gacgacgacg gagccgtgaa
     5821 tctcgaggga gctgggcacg acggtgttgc catcgcgcag cgtgatcgca ccagcgcgag
     5881 cgcggtcgat gtcgacgggc tcgtcgaaga caagctcgat gccctcgtcg gtgatcaggt
     5941 catgcgagtc cgccgcaggc agcgatgaca acagcgtggg cggttggaca tccggcgccg
     6001 gcgacgcctt cagatcggag atcaggtcga gcacgatgtt cgtcggtgcc gagctcatcc
     6061 cgaggaggtt gatgtcgagc gcgccgagtg tctcgatcgc gagcgcgcct tcgtcagcga
     6121 tcgcgaggcc cgtgagctgg atgccgagca cggtctgtgc gagcacggca ttgccagtcg
     6181 gatcggtcgc gtagatcgcg aggtcgaagc tcagatcgac gagcaacggc gaggtcgtgt
     6241 tgtctggaag cgtgccggcc ggctggtacc ggttgcgata caggcggccg ccgccgtcgg
     6301 tgagcatctc gatcgcgatg tcaccggttt cgagaccgga tgggatcgcg cctgcgagct
     6361 ggatcgcgag gcccgcggac gtcaggcgct ggcctttcgg aatcctgaac gcgatcgggc
     6421 cgtcgagcgc gagtgggtcg ccgagctcgg tctcgagcac gttcggtttc atcgccgcgt
     6481 tcgcttcacc gatcagcggg tgcgagacgg ccatcacgtt catgtcgtcg gtgcgcgcga
     6541 cggatgcgac cggatcgccg ctctgccacg tgcgaaacac ctggcggatc ggtccgcggc
     6601 cgagactgtc gagcggcgtg aacgcgatcg ccgtcggcac gaggcgttcg ccgccgacgt
     6661 cggcgagctg attgccgagc cgcagctggt acgtcgcgcc ggctgcgagc ggcgcggccg
     6721 gatcgagcgt gacgtggatg ccgttcgcga ggagcgtcgc cgggacgggc gcgccgttcg
     6781 cgtcgacgag ctcgaccgct ccgttgtcgg tcgtgaccga acgcggatcg agtggttcgg
     6841 agaatacgag ctggagcgtc gagctctcga agatcgggcg gaacgcgccg gggtggagcg
     6901 cgtctaatgt cgcgagggcc gagccattga ccgcgatcag cgatggcgcg cccgcgagtg
     6961 ggcgatcgtt gcgcgtcgtg aaccggaaca gcggctcccc ggccagctcg cccgagccgc
     7021 cacacgtgac ctggtaggtc gtcagcggta gcagcgcgct cgacgtgatc gcgacggtct
     7081 tgccgtgacc gacgacttgt ttggtgatct cgacgttgcc atcggggcct gcgagggtgc
     7141 acccggcctg gtcggcgacc gcatccgagt acgagacgag cacccgcgcg ccgagcggca
     7201 cgtcgagctg gttgtcgact ggataggtga acacaacgct cggaggcggc ggttcgtcga
     7261 cgggaccagc cgagtagcag ccggcgagac agcaggcgaa caggaggggc gggcggcggt
     7321 cgcgcatcgg tttgtgacca ggtatacatg atgtatcccg tgcgtgatag agagtccgat
     7381 gacagccgct cacagtgccg cgcggcgtca cggcccggac cagcgccagc gcagtgatcg
     7441 tccgtggcta ggcgcggccg gcaggcgcgg cttggtcgcc gtcgaaccta cgccagcgtc
     7501 atcggcaggg gcgctacgcg atcgccgtgg cctgcaaccg tgcccgtcgc cacgcgcatc
     7561 atcaccgtgc gtgcgcgcaa gatgctctcg ttcgtgacac ggcgaccttc atcgccttgt
     7621 tcgagcaccg tgcggacgat ctgttcgagc gcgaagacga gcgtccggat gagcagcgga
     7681 tcgaccctct tgctctcgtc ggaagcgtcg tgaaacatcg cgattagcag gtcgtggact
     7741 tccatgcgcc gggcgtggag cggcgattcg aggctctggg cctcaccgcc gaggacgtaa
     7801 acgagacggc ccatcgacct cgcgttgcca aggtgaatgt cgatgcagcg ttcgagctgc
     7861 gtgatgaggt ccttctcgtt cgtgatcgcg cgccggcagg cttcgagcaa gctgctcgtg
     7921 ccgagcgtgt acattgcgag cgcgacgtct tccttgctct tgaagaagcg atagaacgtg
     7981 cgccgcgaga cctggctcgc ttcgaggagg tcttcgaccg agacgccgcg gaaccccttc
     8041 attgcgaata cgcgggtcgc accgaacatg atcatgttgc gcgcgacatc gctcccgagc
     8101 tggacgcccg tcgcgcggcg aggcgcggac gccttcggct tcgtagtctt gaccttcttg
     8161 gctttcccgg ctgccacgaa gccagtatgc cccaatcgcc cgacgacagg cggaaattgt
     8221 gcaaggtgta cacagtgttg ctctcgtcga cctgtccgtc cgataggtag actcggcgtc
     8281 tacctccgac atcggcgtca ctcgcagatg cgcgtagcga cggagatcgt tgcgttgccg
     8341 ccgacgacgt agtccgtgtt ctcgacgaga tacaggtacc ggccgtcgac ggacaggtgc
     8401 agcgcggcat cgtccgcggg tgtgttgagc ggcatgaccc gctcgagcgc gccgaacggt
     8461 gaatcgcgat cggggcgccg cgcagtgacg atgtcgaggt tccctgggct gtcgagatcg
     8521 gtcgcgaacc agatctcgcg ctcgtcggga gagagggtcg gcgacaccgc gaacggcccg
     8581 gcaccctgga tgccaggcac gagcacgggt ccattccaca tcgactggcg cgatgcgcgg
     8641 gtcgcccggt agagcttcgc gttgtttaga tcgggcccga tcccgtagta cagcgcgagg
     8701 ccgtccgcgg tcagccatgg atcgagctgg tgctgcgcgt cggtgccgag ctccgcgatc
     8761 cgcgccggtg ggctccaggc cgtcgtcgtg cttgcccgtt gcgactcgta gagctcgagc
     8821 aaatagggca ggcccatgtc ggccgcgaag atcacgtcga gcccatcggc cgtcaccgtc
     8881 ggctgaaact cggcgccccc cgtgctgagc tccatcacga gctcgggtat gccgtagctg
     8941 ttcggcgcgg tgcccttcgc gacgaagatg ttcgcgccgg cgagcgcggc cgggcggaac
     9001 gaatggaagt agagctcgct gccgttcgcc gtcggcgagc caccccagtc gtcgtagccc
     9061 gcctgatgaa tcatcgcgac gggctgcgcc gcgccgaacg gtttgccgaa gcacgtcgac
     9121 gcgtcgatga cgttcggcac atcgatcgca ccgtcgggtg cgtccacaaa gccaaaccga
     9181 ccacacgcga tgctgctcgc ggttgcgctt gcgaccacca tcgcatgcgg ccagtcagcc
     9241 gagcgcagcc ttctcacgaa gaaacgttat actcgggacg tgactcgatg ggtggcaagt
     9301 ctcctcgtgg tggtgttcgt cggctgtggc ggctcgacgg gtggtggcgg cggcggtgat
     9361 ggcggtggcg gcggcgatga catgccgatc gacgcgccgc ttggaacatg gacctacgtg
     9421 ccgatcgacg gcatggtctg cgccgacggc tcgccgaccg gcattggcgt caatctctcg
     9481 gatacgtcga gcgacgtctt catcttcgtc gctggcggtg gtgcatgctg ggacgcgacc
     9541 acgtgcttca cgacgaagag cgcggtgcat atcgagggtg gctatggcca ggccgacttc
     9601 aacaccgaga tcaatggcct cgcaggcgcg gggatctttc agcgcatcgc gcagaacccg
     9661 ttcaaggatg caagctgggt ctacgtaccg tactgcaccg gtgatctgca cgatggtgca
     9721 aacgtcgcga cgtacgatgc ggcgcatgtc gtgcatcacg tcgggcggac gaacgctgcc
     9781 gcattgctcg cgcgcgtggc ggcgacacgg ccggcggcgc agacggtgtg gctcgtcggc
     9841 gtgtctgccg gcggctacgg catcgcattt gactgggatc tcgcgcgcgc cgaatggccg
     9901 cagagcaagg tccatgcact cgccgattca tcgccgcttg tcgcgatgga gccaacgcgg
     9961 tggagcgcga tgcaggcgtc gtggaagctc acgttcccga ccgcgtgtgc gagctgcacg
    10021 tctgatctcg gcgccatgaa cgcggcgctt cgcagcacgc agcgcccggg cgagcgctac
    10081 gcgctactcg cgaacacacg cgaccagacg atctcgacgt atttcggcgt cacgatggat
    10141 cagttgcaga ccgagacgct cgctgcgcag gcggcgatgt cgccgagcat ggggcaggcc
    10201 gcgtacgtga tcgcgggtac ggggcacgtg ctgctcggca atccgagcgt tcagacgagc
    10261 aatggcgtcg tgcttgcgac ctggtttgcc cagtgggcaa gcggcgatcc ggcatgggcg
    10321 aacgccggcc cttgatcgtt gtcctccagt atacgagttg tatactatcg ccgtgagcac
    10381 gggcgccgcc gacatcctga cccgcgagga gatccggtcg ttcacacagc gttcgaactt
    10441 cgtcggcgcc atcgccatcg cgtggtcgtg gctcgtgatc atcggctgtt tcgtcgcggt
    10501 cgcgctcgtg cctcatccgg cggtgttcgt cggtgcggtg atcctgctcg gcggtcgtca
    10561 gctcgcactc gcggtcgcga tgcacgaagc cgcgcacggc acgctattca agtcgcgctg
    10621 ggcgaacgag gtgctgaccg actggctgtg cgcgcgaccg gtgtggtcgg acaccgcgcg
    10681 ctatcgggtg catcacctcc agcaccacgc gcataccgga accgatcgcg accccgatct
    10741 cggcctcgca ctcgccgagc cgatgtcgcg ccgctcgctg cgtcgcaagc tcttgcgcga
    10801 catcttcgga gcgagcggag tccgccgagt ggtcggcctg ctcgcgatcg acgccgagct
    10861 gctcgagttc gacgttggcg gtagtccgcg ccgggcgaag tttcgctcgc gtggctacca
    10921 cctgcgcgcg ctcgcgaaga acatctggaa gccgatcctc gcgaacctcg tcatgttcgg
    10981 tgcgctctac gcagccggcg tcgcctgggt ctacctcgcc tgggtcgtcg cgtacctcac
    11041 gatgttcagc ctcgtgattc gcgtgcggtc actcgccgag cacgcgtgca ccgcgcgcac
    11101 cgacgaccag ctgctcaaca cgcggacgac gcgtgcaaac cttgcagcgc gcatgacgct
    11161 cgcgccgctc cacgtcaact atcacctcga gcatcacctg ctacccaccg tgccgtggtg
    11221 gcgcttgcct gcgctacatc gcgcgcttgt cgagcgcggt gcgctgcctt cgcagtcact
    11281 cgcccgcgga tatctgcacg tactgcagct agtcagcaga taatagcctc atgcgcgcgc
    11341 gactcgcggt gggtgtcgtt gcactgatcg gctgcggccg gctgggcttc gactccgcgc
    11401 ctggtccgcg cgatgcgcca gcgagctcgg acgtcgacag cccaccagcg taccaagcga
    11461 gcagcgtgcg cttcgatccg gcaggcggcg actatatgtg gacgaacatg ctcgagaaca
    11521 cgccgaattc cggtcgcggc acgttctccg cgtggtttca cttcaatggc ggcgacggcc
    11581 agcaacagct gatcacggtc gcgcagacga tcggcttcgg tggcgtgctg cgcacgacga
    11641 ccaacaagtt tcactttatt ctgcctgcgt gcaccggcat cacggtgctc gacatgcaga
    11701 cgcagaacgc gtacacgagc acgtcgggct ggatccatgt gctcgcagcc tgggacgtga
    11761 ccactggcaa ggccgacctc tacgtcaacg acatcccgga tcgcgccgcc aatccgctga
    11821 tcgtcaacgg ctcgatctgc tacgcggcgc tgcgctgggg cattggcggc tataccggca
    11881 acggtctcga cgccgatgtc gccgatttct actccgcgct cggcacgtac atcgacctga
    11941 gtgtcgaggc gaaccgccgt aagttcatcg acaacaccgg gcggcccgtc gatctcgggc
    12001 ctgcgtgcaa cggcccgacc ggcgcgacct cgaccggctg cttcaccggt gatccggcga
    12061 cctggaacat gaacaaaggc gttgccgccg ggttcacgct cgaaggcgac gggcttgccc
    12121 ccgccgcttc acctagcgat tagctctggt agagccagac gcgcagcagc gacagcagct
    12181 gatcggcgtc gacgggcttg gtgatgtagt cgctcgcgcc ggcctcgagg cacttctcgc
    12241 ggtcgccctt catcgccttg gcggtcaacg cgatcaccgg caggtccttc cactcggaac
    12301 gctcgcggat gcgacgcacg gcttcatagc catccatctc cggcatcatg atgtccatga
    12361 ggacgacatc gatgctcggg ttctcctcga gcttcgcgag cgcgcgcttg ccgttctcgg
    12421 catacagcac gttcatcttg tgaccttcca ggagcgatgt cagcgcgaag atgttgcgga
    12481 gatcgtcgtc gacgacgagc accgtgcgct ccgcgagctg cggatcgctc ttcgcgagtc
    12541 gacgcagcac gcgctgcttg cgctcgggga ggttggcctc cacacgatgc aggaacagcg
    12601 cggtctcgtc gaggagccgc tccattgtgg tcacgtcctt gatgatgacg gtctgtgcga
    12661 ggccgtgcag acgctggcga tcctcgtcgg tgagatcacg gccggtgtac acgatcaccg
    12721 gcaagcgccg gtgctgcggg tcggccttga tctgctcgat gaggtcaaag ccactcatgc
    12781 cgggcaggcg aagatcgagc acgacgcagt cgtatggctt ctccgccaac gtctgcagtg
    12841 cgatttcacc cgacgcgacg gtcgtggtct cgacatcacc atcaccgatg aggtcgctga
    12901 tcgcctggct ctgcaccgga tcgtcctcga cgacgagaag ctgcttgacg cgcttctcga
    12961 caaacccgcg gaccttgtcg agccctgctt tgaggtcctc ctcggtgacc ggcttctgga
    13021 ggaagccgtg cgcgccgctc tgcaggccgc gccgttcctc gtcgagaccc gacacgatgt
    13081 gcaccgggat gtgacgcgtc gccgcgtcgt gcttcagccg atcgagcagc acccaaccgt
    13141 cgaggtcggg caaccgcaga tcgagcgtga tcgcatccgg cttgatcgag cgcgcgagct
    13201 cgagcgcctg gtgaccggtc atggccacaa cgcccttgaa gctcgacgcg cgtgccatct
    13261 cgagcatcgt catcgcgaac gtcacgtcgt cttcgacgat caacagcacg cggtctccag
    13321 gctcgaggct ctcgtagtcg tccggcaccg tgcgtgtgag cggcggcgga ggcggagctt
    13381 cctccttgtc gaccgattcg ggctgcgtga ggtcgatgac ctgcggcagc tgcggcaccg
    13441 gctgtggaac gcgccgcacg ctcggatcgc gctgcttgta cacggtcggc aggtacagcg
    13501 tgaacgtgct gccctgaccg atgaccgagt cgacgcgcag ctcgccaccg agcagaccgg
    13561 cgatctcgcg tgaaatcgcg aggccgaggc cggtgccgcc gtacttacgg ctcgtcgaac
    13621 cgtccgcttg cgtgaacggt tcgaagatca ggcgctgctt atccgcgggg atgccgatgc
    13681 cagtatccgc caccgagaac gcgatgacgt cgttcgacgt cttcgtgatc cgcagcttga
    13741 cgcggccacg ctcggtgaac ttgatcgcgt tcgacaagag atttcgcagc acctggcgca
    13801 agcgcatgtc gtcggtctcg atcgagccgg gcaggtgctc gtcgagctcg acggagaact
    13861 gcagcccctt ctcttcggcg acctggcgga acgtctggtc gacgtagtcg cgcaggtttg
    13921 cgcaacgcac cggtccgatc tcgaccgcca tcgtgccgga ctcgatcttc gcgagatcga
    13981 gaatctcgtt gatgagggtc atcaggtcgg tacccgcctg gcggatcgtc tccgcgtagc
    14041 ggatctgctt gtcggtcagg ttctgctcgg cgttgtcggc gagctgacgg ctgaggatga
    14101 gcagcgagtt gagcggcgtg cgcagctcgt gggacatgtt cgcgaggaac tggctcttgt
    14161 acttcgacgt gagggccagc tgctccgctt tctcttcgag ctcctgacga gcgagctcga
    14221 cctggcggtt cttcctctcg acttctgcct tctgctcggt gagctgatgc gctttctcct
    14281 cgagctcctc gttcgtctgc tgcagctcgt cctgctggct gcgcagctcc tcggtgaggc
    14341 tctgcgactg acgcagcagc tcgtcggtac gcatcgtcgc ttcgatcgtc gcgatcacga
    14401 tgccgagtga ctcgagcatc tggtcgagga acgcgagctg gatgccgctg aacttctcga
    14461 agctcgccag ctcgatgacg cctttgacct caccctcgaa caggatcggc acgacgacga
    14521 tcgagtgcgg cggcgcttcg ccgagagcac tggtgatctg gacgtaatcc gcgggcacct
    14581 tgccgacgac aatgcgcttg ccctcgagcg ccgcctggcc gacgagcccc tggccgaatc
    14641 gccacacgtt cgcgacggcc ttgcgctcct ggaacgcata gctcgcgaac agccgcagga
    14701 tctggccttc gtcgtcggct tctgccatgt agaacgcgcc gtggtgggcg ttcacgagtg
    14761 gagccagctc gttgaggatc tggcgcgcga cggtgagcag atcgcgctgg ccctgcagca
    14821 tacgggtgaa ccgcgcgacg ttcgtcttca gccagtcctg ctcttggttg accttcgtgg
    14881 tctccgcgag cgtgcggatc atctggttga cggtgtcttt cagctgcgcg atctcgccgc
    14941 gcgcttcgac ggtgatcgac cgggtcaggt cgccttgcgt cacggcagtc gcgacgtcgc
    15001 gaatcgcgcg gacctggttt gtcaggttgc cggcgagctc gttgacgttg ttcgtcagat
    15061 cacgccacac accggcagcg ccgggcacgt ctgcctggcc accgaggcgg ccgtcgacac
    15121 cgacgtcacg cgccacgccg gagacctgtg ccgcgaacgt cgagagcgtg tcgatcatgt
    15181 tgttgatcgt gtcgaccagc gtcgcgatct caccacgggc ggcgaggttc agcttgcgat
    15241 cgaggtcgcc gttcgcgacc gcggtgacga cgttcgcgat gccgcgcacc tgctcggtca
    15301 ggttgcgcgc catcacgttg acgttgtcgg tcagctcgcg ccacgtgccg gacacgccgc
    15361 gcacctcggc ctggccgccg agcacgccgt cgatgccgac ctcgcgggcg acgcgcgtca
    15421 cctcggacgc gaacgagccg agctgatcga ccatcgtgtt gatcgtgttc ttgagctgga
    15481 gcagctcgcc gcggacctcg acggtgatct tccgggtcag gtcgccattc gcgacggcgg
    15541 tcgtcacgta ggcgatgtcg cggacctggt tcgtcaggtt cgacgccatc tggttgacgt
    15601 tgtcggtcag ctccttccac gtgccggcga cgccgcgcac cgccgcctgg ccgccgagct
    15661 tgccctcgac gccgacgtcg cgcgcgacgc gcgtcacctc gtcggcgaac gacgagagct
    15721 ga
//
LOCUS       DASUFX010000128         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_298313,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000128 DASUFX010000000
VERSION     DASUFX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298313"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VFV99_04945"
     CDS             <1..1698
                     /locus_tag="VFV99_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5058683.1"
                     /translation="DRPPCNGRLEDCVRDATTRQSRLAKLQAAQRAEACRGDSCTAQW
                     QDARRIYTALAGFEDTGPAALLGLARVSGRLGDSKGVQGALSDLELRYGDRTVDELPA
                     RLVVAIMRAEKDRGALLEVAEAIVAGTYDVHPVVGAGVLAQLRQRLDGPMAPDLAARR
                     ATLDEQMTRIKSEARAAAGLADDVQDIMRDATLAWRGKPAQKQPARTLIYKKRADGSV
                     IGMAVDAAMLEQAAGHAPSDNVAVHVQHLVLPVGQSPPSDVWRQLTLKPFPQGLSHLT
                     LHLVNPRKDRDPLDAVLSARANRHIMYTSALAIMLGLGILATIRGAARARELAQLKSD
                     FVSTVSHELKTPLTSIRMFAEMLEQGVAQGDAAKMARYHGVIVQESQRLGLLIANLLD
                     YSQIERGTRRYTPSRESVEKLARHAVTTFETLRDPEKGKRNPIELIVSPEAMKTDVEV
                     DRDVVVQAVLNLLANAVKYGAADKAIEVTVAADDFAATIAVTDHGPGIPAHEQARIFR
                     EFYRTPDAYRSGVEGTGLGLALVKRHIEALGGSVDVQSTVGHGATFTIRLPRVEAKA"
     gene            1695..2426
                     /locus_tag="VFV99_04950"
     CDS             1695..2426
                     /locus_tag="VFV99_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5058684.1"
                     /translation="MTRILVIEDDPSIRMGLEDTLTAKGYQVEVVGKGGEGAEKAMSG
                     RYDLIVLDVMLPDIDGFEVCRRIRQSRGPTRRLPVIMLSARGAELDRVRGLELGADDY
                     VTKPFSLMELLARVASVLRRAQGESAEPIGLAFGDIDIDLVGQVALRSGKRIELPSRA
                     FAILKVFARRPGEVVSRDVLLDEAWGYEDYPNTRTVDNHLVKLRRALEDEPDKPRFLV
                     TIHGAGYKLDVPKDAVRWAGAVTRT"
     gene            2579..>2771
                     /locus_tag="VFV99_04955"
     CDS             2579..>2771
                     /locus_tag="VFV99_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058685.1"
                     /translation="MLLALVACGDDQQRAATHVDSPVRGSGGADLAAGMKTELDGDAP
                     AFGDGVLAELTGDDVAARAS"
BASE COUNT          474 a          905 c          963 g          429 t
ORIGIN      
        1 gatcggccgc catgcaatgg ccgcctcgaa gactgcgtgc gcgatgcgac gacgcgccag
       61 tcgcgactcg cgaagctgca ggctgcgcaa cgcgcggaag cgtgccgagg cgattcgtgc
      121 accgcgcaat ggcaggacgc gcgccgcatc tacacggcgc tcgcgggctt cgaggatacg
      181 ggaccggccg cgctactcgg cctcgcgcgc gtctccggcc ggctcggcga cagcaagggc
      241 gtgcagggcg cgctctcgga tctcgagctg cgctacggcg atcgcacggt cgacgagctg
      301 ccggcgcgcc tcgtcgtcgc gatcatgcgc gcggagaagg atcgcggtgc gctcctcgaa
      361 gtggcggaag cgatcgtcgc cggcacctac gacgtgcatc cggtcgtcgg cgccggtgtg
      421 ctcgcgcaac tgcgccagcg gctcgatggc ccgatggcac ctgacctcgc ggcgcggcgc
      481 gcaacgctcg acgagcagat gacgaggatc aagagcgagg cccgtgcggc agcggggctc
      541 gccgacgacg tgcaggacat catgcgcgac gcgacgctcg cgtggcgggg caagccggcg
      601 cagaagcagc ccgcgcgcac gctgatctac aagaagcgcg cggacggcag cgtgatcggc
      661 atggctgtcg acgccgcgat gctcgagcaa gccgcaggcc acgcgccgag cgacaacgtc
      721 gcggtgcacg tgcagcatct cgtgctgccg gtcggtcagt cgccgccgag cgacgtgtgg
      781 cgccagctga cgctgaagcc gttcccacaa gggctgtcgc acctcacgct gcatctcgtg
      841 aatccgagga aggatcgcga tccgctcgac gcggtgctta gtgcgcgcgc caaccggcac
      901 atcatgtaca cctccgcgct cgcgatcatg ctcgggctcg gcatcctcgc gacgattcgt
      961 ggcgccgcgc gtgcgcgcga gctcgcgcag ctcaagagcg acttcgtgtc gacggtctcg
     1021 cacgagctga agacgccgct gacgtcgatc cgcatgttcg ccgagatgct cgagcagggc
     1081 gtcgcccagg gcgatgcggc gaagatggcc cgctatcacg gcgtgatcgt ccaggagagc
     1141 cagcggctcg gcctgctgat cgcgaacctg ctcgactact cgcagatcga gcgtgggact
     1201 cgtcgctaca cgccgagccg cgagagcgta gagaagctcg cgcgtcacgc agttacgacg
     1261 ttcgagacgc tgcgcgaccc cgagaagggc aagcgcaatc cgatcgagct gatcgtgtct
     1321 ccggaagcaa tgaagacgga tgtcgaagtc gaccgcgatg tcgtcgtgca ggcggtcctc
     1381 aacctgctcg caaacgccgt gaagtacggt gctgccgaca aggcgatcga ggtcacggtc
     1441 gctgccgatg atttcgcggc gacgatcgcg gtgaccgatc acggtcccgg cattcctgca
     1501 cacgagcaag cgcgaatctt ccgcgagttc tatcgcacgc ccgatgcgta ccgctcgggt
     1561 gtcgaaggaa ctggccttgg cctcgcgctc gtcaaacgcc acatcgaagc actcggtggc
     1621 agcgtcgacg tgcaatcgac cgtcggtcat ggcgcgacgt tcacgattcg actaccgcgt
     1681 gtggaggcaa aagcgtgacc cgcatccttg tcatcgaaga cgatccgtcg atccgcatgg
     1741 gcctcgagga cacgctgacc gcgaagggct atcaggtcga ggtcgtcggt aaaggcggcg
     1801 agggcgccga gaaggcgatg tcgggtcgct atgacctgat cgtcctcgac gtgatgctgc
     1861 ccgacatcga cggtttcgag gtgtgtcgac ggattcggca gagccgcggc ccgacgcgcc
     1921 ggttgccggt gatcatgctg tccgcgcgcg gtgcggagct cgatcgcgtg cgcggtctcg
     1981 agctcggcgc cgacgattac gtcaccaagc cgttctcgct gatggagctg ctcgcacggg
     2041 tcgcgtcggt actgcgtcgc gcgcaaggcg agtcagccga gcccatcggc cttgcgttcg
     2101 gcgacatcga catcgatctc gtcggccagg tcgcgctgcg cagcggcaag cgcatcgagc
     2161 tcccgagtcg cgcattcgcg atactcaagg tgttcgcgcg gcggcccggt gaagtcgtca
     2221 gccgcgacgt gctgctcgac gaagcgtggg gctacgagga ctatccgaac acgcggaccg
     2281 tcgacaatca cctcgtgaag ctgcgccgcg cgctcgaaga cgaaccggac aagccgcgct
     2341 tcttggtgac gatccacggc gctggctaca agctcgatgt tccgaaggat gcagtgcgct
     2401 gggcgggcgc agtgacaaga acatgacaag cttttgcgag agcgtgacgg tgcgatgcga
     2461 agcggccatc agccgcgctc gtctccttgt taccaaggat atgggcgagg cgatggcgcc
     2521 ggcatcagag agccaacctt ccggtgggac acgctctggt gccggcgccg cgctgattat
     2581 gctgctcgca ctcgtcgcgt gcggcgacga tcaacagcgc gccgcgacac acgtcgactc
     2641 tccggtgcgt ggcagcggtg gcgccgatct cgcggccggt atgaagaccg agctcgacgg
     2701 cgacgcgcct gcattcggcg atggcgtgct cgccgagctc acgggcgatg acgtcgcggc
     2761 gcgcgcgtcg t
//
LOCUS       DASUFX010000129        10030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_159784,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000129 DASUFX010000000
VERSION     DASUFX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159784"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="clpX"
                     /locus_tag="VFV99_04960"
     CDS             <1..1158
                     /gene="clpX"
                     /locus_tag="VFV99_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5058686.1"
                     /translation="KLISGPRVFICDQCVTLCNDILAKEEASERPKYPPPRAIVEELD
                     RYVVGQKDAKRALSVAVYNHYKRIGLRGKQTDVELQKGNILLIGPTGSGKTLLAQTLA
                     KKLDVPFAIADATTLTEAGYVGEDVESVVKALFRAAGGDVEKTARGIICIDEIDKIAR
                     KGGTPSPTRDVSGEGVQQALLKILEGKTATITPDGARNRPQQELIQIDTTDILFICCG
                     AFNGLEDIVRRRTGQRGLGFGAEVSTAEDDKDALRRQARTEDLIKFGMIPEFMGRLPV
                     IVSCDALDVDALTEVLWKPKNALAKQYQRLFELEGVKLNFRPDALKAIAEEASRRNSG
                     ARGLRAILEEVMLDVMYEIPSLSGVKECVVTREVVEHRARPELVLESKAAS"
     gene            complement(1312..2220)
                     /locus_tag="VFV99_04965"
     CDS             complement(1312..2220)
                     /locus_tag="VFV99_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058687.1"
                     /translation="MRAWLCAAVLAAAACGGGGGSTGDDDQPDAAVDGSVTPGWTSLI
                     ERSWSLDPTVEGFKCIRIKIEQDTYVNAFHVKSPPGTHHELLTISTTPSPIGEYECDA
                     LNLDKQMVFAGGIDTNDLQFPAGVAIKLAAGTYINLNLHVANFTDSPISGTSGIEVKT
                     LKASEVVNEAEAMFLGTFNIHILPGATNQMVPGSCVAPTEWRILDLWPHMHSYAKHQN
                     VKVARSTGPVETLLNVDYTYMEQKNYPMTNVAIHPGDELQVECYYDNPTQQEVTYGDS
                     AVAEMCFAGFYTYPANGVDKYFCSQL"
     gene            2333..2746
                     /locus_tag="VFV99_04970"
     CDS             2333..2746
                     /locus_tag="VFV99_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5058688.1"
                     /translation="MPELRNVIVFAIGAARYAVELRWVREVVTLGFVTAVPTAHPVLG
                     GVCNLHGTILPVLDVGALLDQPAGPAPRQGDGALVIEADGTLCGLRVDQVDHVASLFE
                     DGGAVVDAAGRPLTLLDPQRLLRRALELVSSAVPA"
     gene            2743..5007
                     /locus_tag="VFV99_04975"
     CDS             2743..5007
                     /locus_tag="VFV99_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058689.1"
                     /translation="MSDTDLDFDPDELAMLRQLFRSEAHDALEAVTARVLATGSAKPT
                     GETLTEMMRVTHTLKGAAGTVGLNVMVELAHRLESAFAALGRETAPWTASTADLIVEV
                     TDGLRGYLDQLAVEPAAAEPLAQRIRDQIDRVARTRDSAPDVTLPPEKSDPPPDRPSL
                     SEMMSPNESLSMPTITELPAPEGLPEPILDIPSTDLGVPEPKSYLRVEPERIDALMSS
                     AGELLFDRTRIERRVQLLRTLARDLARTRQSLRDAIATDTKSKDARSLSDAENELASQ
                     ATQLSQTTAALLDEIEALRRTIGELQRGLVRIRMESARNLMTHAARTLRALRRATGVR
                     VELRTLGEETEFDKAVAEQLVDPITQLLRNAVAHAIERPEERAARGKPPSATITIRAR
                     QDGNLLVLEVSDDGRGVDTAALRDRLVATGRWSQARAQLATDADVLDALGTGVSVRGD
                     ADELAGRGIGLDLVRQTIARLGGEVKVISAPGRGTTFSLRLPLSTSLAQAMLFKVGGQ
                     VYAIPAVHVSETTIVDANASTATVRQVPVPVLRLESLLGQSTGNERRPGVVVSFAGKT
                     MVCSVDKIVGPREIIIKPLGPLLAPLTLYAAATISGSGKVQLILDPAQLVRRVYPDAH
                     EYQDPTSGPMVLAGRALVVDDSRAIREAMTSMLGREGWIVDVAEDGARALQMTRQLSY
                     DLVVTDLEMPELSGFELIARLRKDQRFATTPIVIITSRANPEHRRRAKDLGVRALVAK
                     PITRRKLLEALAAR"
     gene            complement(5332..8103)
                     /gene="rnr"
                     /locus_tag="VFV99_04980"
     CDS             complement(5332..8103)
                     /gene="rnr"
                     /locus_tag="VFV99_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX5058690.1"
                     /translation="MQPIDRQAILDSLSRLVTPGIKLTALADELGLRKQDYSALRAQI
                     LELVEEGYVNVLPGGAFALSPQGRKADPHAKPVPGGPQASGPRPQPEKKMKPTKAAKE
                     SRRKPEAKLPWKQGALRVVPPKASPAPREEYDPETGQIRVVDLPPREDLVSKPPAAER
                     TGKVPEGTAETRIPEARGLRPEAGGLVGRITVHPAGYGFVATEEGTVFVPAKYRGTSM
                     DGDQVAVETWPGVRGTEGRVVDVLKRGRARITGILRRAGRAIYLEPDDPRIAADFGRA
                     VIYDAPYGKDGDAIVVEITRYPDAYNKELGGNVLKVLGDPEDPRTEIEKILACAVIPL
                     EFPDDVLAQAKATSQELTPADLADRIDLRDRRFCTIDPETARDFDDALCIEDGPHGGP
                     RVWVAVADVSHYVRWNDPLDREAAIRGVSVYLPDRVIPMLPKELSAGICSLNPLVDRC
                     AMVVRLDYMPNGDIVDIGYSAAAIRSKARLDYPGVAAALEGDFRGRREQYRPWIDELT
                     KLNTLARTLRTRRQARGALELEIAEPKVVLDADDPRLVRDVVKAKGDPAVKQAYELVE
                     EFMIAANEAVGTFFRKRGAPTVWRVHAPPDPDRVAQLAELFNAYGIRVDVEEATTPLG
                     MKAVLDQVAQKPGAQALSFLVLRTLTQAVWDTVPIGHFGLASGDYLHFTSPIRRYPDL
                     LVHRLLKHHLHRDGNASGGGYAKPPPNVETLAQLAADSSLHERRAMEAERESVAMYRA
                     YLMRDQVGQRFEGTVSAVTSFGAFVEIEEPFVEGLIKLDSLGEPMDYDEVHMRLSGKR
                     TGKTIELGDKVLVDINNVSVLRRRIDFTLIATATRKGKPPKLEASGKAGIAQRKKAGR
                     EVERAQLGRVERGAQPRGRAGARGGGGGGGGALRLGVSHRGMRDGGGGGGGGRKKGGG
                     GHKGKRGKR"
     gene            complement(8124..9407)
                     /locus_tag="VFV99_04985"
     CDS             complement(8124..9407)
                     /locus_tag="VFV99_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5058691.1"
                     /translation="MRVWKAAAVAVAVVELACSGTQARQTGCSKDTDCKDPRVCEKGV
                     CVDPHPVGQSQSVRPTTRDGGVEAAPVKGPPAFAMFGGDARHTGRRGGPAPVQAPKEL
                     WSVDVKGVVAGSPTIGPDGTIYVTSHEGSLFAIDPKGSIKWSFKTGDRSWSTPAVAKD
                     GTIYVGSDDDHLYAVKSDGSLKWKLRLGDCDPKGFGPESSRCDVDGGPTIGPDGTIYV
                     GGDGIHAVWADGTLRWKLATSEHVASTPALATDGTVYAGSEDDALYAVAPDGTKKWEL
                     RTGGDVDATPAIGADGTIYVGSDDHTIYAVSPKGELQWRVITGEDVRGGAAIGNDGTI
                     YVGSYDKKLYAISQSGRVQWKLAAADKISSTPGIASNGLILFGAQDEHLYAVAPDGTL
                     RWHLALAADVDTTPAIASDGTLYVAGDDGHLHAFR"
     gene            complement(9441..9716)
                     /locus_tag="VFV99_04990"
     CDS             complement(9441..9716)
                     /locus_tag="VFV99_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058692.1"
                     /translation="MAKLVDGFKDGKPELGRRTFLVPENHSEVLALLYHEQTGIVGGL
                     PVGPVTTDPFGAHGIRMYPDQDPVVLEETRRYFEKLAERFSNDLPKA"
     gene            9850..>10030
                     /locus_tag="VFV99_04995"
     CDS             9850..>10030
                     /locus_tag="VFV99_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5058693.1"
                     /translation="MRLELWFDFSCPYAYLASRRAPHVARAAGVELDYKPMLLGGVFR
                     GIGAGDGPMATLGPAK"
BASE COUNT         1614 a         3416 c         3285 g         1715 t
ORIGIN      
        1 aagctcatct ccggcccgcg cgttttcatc tgcgatcagt gcgtgactct gtgtaacgac
       61 atcctcgcaa aagaggaggc gtcggagcgg cccaagtacc cgccaccgcg ggcgatcgtg
      121 gaagagctgg accgctacgt agtcggccag aaggacgcca agcgcgccct ctcggtcgcc
      181 gtctacaacc actacaagcg catcggcctg cgcggcaagc agacggatgt cgagctccag
      241 aagggcaaca tcctcctcat cggcccgacc ggctcgggca agacgttgct cgcgcagacc
      301 ctggccaaga agctagacgt gccgttcgcg atcgcggacg ccaccaccct caccgaagcc
      361 ggctacgtcg gcgaggacgt cgagagcgtg gtcaaggcgc tgttccgcgc tgccggcggc
      421 gatgtcgaga agaccgcacg cggcatcatc tgcatcgacg agatcgacaa gatcgcacgc
      481 aagggcggca cgccgtcgcc gacgcgtgac gtctcgggcg aaggcgtgca gcaagcgctg
      541 ctcaagatcc tcgaaggcaa gaccgcgacg atcacgccgg atggcgcgcg caatcggccg
      601 cagcaagagc tcatccagat cgatacgacg gacatcctgt tcatctgctg cggtgccttc
      661 aacggcctcg aggacatcgt ccgccgccgc accggtcagc gtggcctggg cttcggcgcc
      721 gaggtctcga cggccgagga cgacaaggac gcgctgcgcc gccaggcgcg cacggaagac
      781 ctgatcaagt tcggcatgat ccccgagttc atgggacgtc tgccggtgat cgtgtcgtgc
      841 gacgcgctcg acgtcgatgc gctgaccgag gtgctctgga agccgaagaa cgcgctggcc
      901 aagcagtacc agcggctgtt cgagctcgaa ggcgtgaagc tgaacttccg ccccgacgcg
      961 ctcaaggcga tcgcagagga agcgtcgcgc cgcaactcgg gtgcgcgtgg tctccgcgcg
     1021 atcctcgagg aagtgatgct cgacgtgatg tacgaaattc cgtcgctctc gggcgtcaag
     1081 gaatgcgtcg tcacccgcga agtcgtcgag caccgcgctc gccccgagct cgtcctcgag
     1141 tcgaaggctg cgagctaaga acgcccgccg ccatcacgta ggcgcaagcg taagcggccc
     1201 aggtgctgcc cctgcgtcgc gcccgtcgaa cgacgcgcgc cgaccacgtc ggtgatcgtg
     1261 aagtcgacgc tgcaccggcc gcaccgagct cgcagtcgat tcgccacggc actacagctg
     1321 cgagcagaag tatttgtcga cgccgttcgc cggatacgtg tagaagccgg cgaagcacat
     1381 ctcggcaact gcactgtcgc cgtacgtgac ctcttgctgc gtcgggttgt cgtagtagca
     1441 ctcgacctgg agctcgtcgc ccgggtggat agcgacgttc gtcatcgggt agttcttctg
     1501 ctccatgtac gtgtagtcga cgttgagcag cgtctcgacg gggccagtcg agcgcgcgac
     1561 cttcacgttc tggtgcttcg cgtagctgtg catgtgcggc cagaggtcga gaatgcgcca
     1621 ctcggtcggc gccacgcagc tgccgggcac catctggttc gtcgcgccgg gcaagatgtg
     1681 aatgttgaac gtgccgagga acatcgcctc ggcctcgttc acgacctcgc tcgccttcag
     1741 cgtcttcacc tcgatacccg acgtgcccga aatcggcgag tcggtgaagt tcgcaacgtg
     1801 gaggttcaag ttgatgtacg tgccggcagc gagcttgatc gcgacaccgg ccgggaactg
     1861 cagatcgttc gtgtcgatgc cgcctgcgaa caccatctgc ttgtcgaggt tgagcgcgtc
     1921 gcactcgtac tcgcctatgg gactcggggt ggtcgaaatc gtgagtagct cgtgatgcgt
     1981 gcccggcggc gacttgacgt ggaacgcgtt gacgtacgtg tcctgctcga tcttgatgcg
     2041 aatgcacttg aagccttcga cggtcggatc gagcgaccag ctgcgttcga tcaggctggt
     2101 ccagccgggt gtcacgctgc catccaccgc tgcgtccggt tggtcatcgt cgccggtcga
     2161 gccgccgccg cctccgcagg ccgccgccgc caaaaccgcc gcacacaacc aagctcgcat
     2221 atggttgcac tctacaccca ggcctgcggt gtcgagcatt ttgttcgcga tcgggtgttc
     2281 tcgctcacgc tggccgcagc atggggttca gaggccgcgc tatgctgctt ccgtgcccga
     2341 gctgcgcaac gtaatcgtgt tcgccattgg tgcagcacgt tatgcggtcg agctgcgctg
     2401 ggtgcgagaa gtcgtcacgc tcggcttcgt gacggcagtt ccgacggctc acccggtcct
     2461 cggtggtgtc tgcaatttgc acggaacgat tctgccggtg ttggacgtag gtgctttgct
     2521 ggatcaaccg gccggaccag cgccgcgcca gggtgatggc gcactggtca tcgaggccga
     2581 cggcacactg tgcgggctgc gcgtcgatca ggtcgatcac gtagcgtcac tgttcgaaga
     2641 tggtggtgcg gtggtggatg cggccgggcg gccgctcacg ctactcgacc cgcagcgcct
     2701 gctccggcgc gcgctcgagc tggtcagctc ggcggttccg gcatgagcga caccgatctc
     2761 gatttcgatc ccgacgagct cgcgatgttg cgccagctgt ttcgcagcga agcgcacgac
     2821 gcgctcgaag cagtgaccgc gcgcgtgctc gcgaccggct ctgccaagcc gaccggcgag
     2881 acgctgaccg agatgatgcg cgtgacccac acgctcaaag gcgcggccgg cactgttggc
     2941 ctcaacgtga tggtcgagct cgcacaccgg ctcgagagcg catttgccgc gctcggtcgc
     3001 gagaccgcgc cgtggaccgc atcgaccgcg gacttgatcg tggaagtcac ggatggcctg
     3061 cgcggctatc tcgatcagct cgccgtcgag ccggcagcgg ccgagcccct cgcgcagcgc
     3121 attcgcgacc agatcgatcg cgtcgcgcgc acgcgcgatt ccgcgcccga cgtcacgctg
     3181 ccgcccgaga aatccgaccc gccgcccgac cggccatcgc tcagcgagat gatgtcgccg
     3241 aacgagtcgc tgtcgatgcc gacgatcacg gagctgccgg cgcccgaagg cctgcccgag
     3301 ccgatcctcg atatcccgtc gaccgacctc ggcgtgcccg agccgaaatc gtacctgcgc
     3361 gtcgagcccg aacgcatcga tgcgctgatg tcgagcgccg gcgagctgct cttcgaccgc
     3421 actcgcatcg agcgccgcgt ccagctgctc cgcacgctcg cgcgcgacct cgcacgcacc
     3481 cggcagagcc tgcgcgacgc gatcgccacc gatacgaaga gcaaggacgc acgcagcctg
     3541 agcgacgccg agaacgagct cgcgagccag gcgacgcagc tctcgcaaac cacggccgct
     3601 ctgctcgacg agatcgaagc gctgcgccgc acgatcggcg agctgcagcg cggcctcgtg
     3661 cgcattcgca tggagagcgc gcggaatctg atgacgcacg cggcgcgtac gctgcgcgca
     3721 ttgcgccgtg cgaccggcgt ccgcgtcgag ctgcgcacgc tcggcgaaga gaccgagttc
     3781 gacaaagctg tcgccgagca actcgtcgat ccgatcacgc agttgctccg caatgcggtc
     3841 gcccacgcga tcgagcgccc cgaggaacgc gccgcacgcg gcaagccacc gagcgcgacg
     3901 atcacgatcc gcgcacgcca ggacggcaac ctcctcgtcc tcgaagtctc cgatgacggt
     3961 cgcggcgtcg acaccgccgc gctccgcgat cgcctggtcg cgaccggccg gtggtcgcaa
     4021 gctcgcgccc agctcgcgac cgacgccgat gtgctcgacg cgctcggcac cggtgtctcg
     4081 gtgcgcggcg acgccgacga gctcgcgggc cgcggcatcg gcctcgatct cgtgcgccag
     4141 acgatcgcgc gcctcggcgg cgaggtgaag gtcatctccg cgcccggccg cggcacgacg
     4201 ttcagcttgc gcctgccgct gtcgacatcg ctcgcgcaag cgatgctgtt caaggtcggc
     4261 ggccaggtct acgcgattcc cgccgtgcac gtcagcgaga cgacgatcgt cgacgcgaac
     4321 gcatcgaccg cgaccgtgcg ccaggtgcca gtgcccgtgc tgcgcctcga gagtctgctc
     4381 ggccagagca ccggcaacga gcgccggccc ggcgtcgtcg tgtcattcgc gggcaagacg
     4441 atggtgtgca gtgtcgacaa gatcgtcggc ccgcgcgaaa tcatcatcaa gccgctcggt
     4501 ccactgctcg caccgctcac gctgtatgcc gcggccacga tcagcggctc gggtaaggtg
     4561 cagctgatcc tcgatcccgc gcagctcgtc cgccgcgtct atccggacgc acacgagtac
     4621 caggatccga cgtcgggacc gatggtgctc gccggccgcg cgctcgtcgt cgatgattcg
     4681 cgcgcgattc gtgaagcaat gacctcgatg ctcggccgcg aaggctggat cgtcgacgtc
     4741 gccgaggacg gtgcgcgcgc gttacagatg acgcgccagc tgagctacga cctcgtcgtt
     4801 accgatctcg agatgcccga gctgtcgggc ttcgagctga tcgcgcgcct gcgcaaggac
     4861 caacggttcg ccaccacgcc gatcgtgatc atcacgtcgc gcgcgaatcc cgagcaccgc
     4921 cgccgcgcga aagacctcgg cgtacgcgcg ctcgtcgcga agccgatcac gcggcgcaag
     4981 ctgctcgaag cgctggccgc ccgctagcgc tgcgaccgcg agctcctcat acgcttgcga
     5041 tcaatcgtcg atctcctcgt cggccgcagc ctcgtgccgc tcaggttgcg ctcggcccag
     5101 aaaaacgcca agaacgccaa gatctcgcca agaacgccac cagaggatcg tctcaggatc
     5161 gcgttctgct cgagctcgcg ttcgagcaga gcgcgcgctc cccaaaggct tcttgccttg
     5221 gcgttcttgg ccggcttggc atcttggcgt ttactctggg agctgcgccc tgaagctgac
     5281 gctgaggccg tactcgacgc gggcgggctc gtcgcgttct gcgcccaggt actaacgctt
     5341 gccgcgcttg cccttgtggc cgccgccgcc cttcttgcgg ccaccgccgc cgccgccgcc
     5401 gccgtcgcgc atgccgcgat gcgagacacc gagacggagc gcaccgccac cgccgccacc
     5461 accgccacgt gcgccagcgc gaccgcgcgg ttgcgcacca cgctcgaccc ggccgagctg
     5521 cgcacgctcg acctcgcgcc ctgccttttt gcgttgcgcg atgcctgctt tgccgcttgc
     5581 ttcgagcttc ggcggcttgc ccttgcgggt ggcggtcgcg atcagcgtga agtcgatgcg
     5641 ccggcggagc acggacacgt tgttgatgtc gacgagcacc ttgtcgccga gctcgatcgt
     5701 cttgccggta cgcttgccgg acagacgcat gtgcacctcg tcgtagtcca tcggctcacc
     5761 gagcgagtcg agcttgatga ggccctcgac gaacggctcc tcgatctcga cgaacgcgcc
     5821 gaagctcgtg acggcagaga ccgtaccctc gaagcgctgg ccgacctgat cgcgcatcag
     5881 gtacgcgcgg tacattgcga ccgactcgcg ctcggcttcc atcgcgcgcc gctcgtgcag
     5941 cgaggaatcg gcggcgagct gcgccagcgt ctcgacgttc ggcggcggtt tggcataacc
     6001 gccgcctgac gcgttgccat cgcggtgcag gtggtgcttg aggaggcggt gcacgagcaa
     6061 gtcgggatag cggcggatcg gcgacgtgaa gtgcaggtag tcacccgacg cgaggccgaa
     6121 gtggccgatc ggcaccgtgt cccagaccgc ttgcgtgagc gtgcgaagca ccaggaacga
     6181 gagcgcctgt gcgcccggct tctgcgcgac ctgatcgagc acggccttca tgccgagcgg
     6241 cgtggtcgct tcctcgacat cgacccgaat gccgtacgcg ttgaacagct cggcgagctg
     6301 ggcgacgcga tccggatcgg gcggcgcgtg cacgcgccac acggtcggcg cgccgcgctt
     6361 gcggaagaac gtgccgaccg cctcgttcgc ggcgatcatg aactcttcga cgagctcgta
     6421 cgcttgcttg accgccgggt cacccttcgc tttgacgacg tcgcgcacga gacgcggatc
     6481 gtcggcgtcg agcacgacct tcggctcggc gatttccagc tcgagcgcgc cacgcgcttg
     6541 gcggcgagtg cgcagcgtgc gcgcgagcgt gttgagcttc gtgagctcgt cgatccaggg
     6601 ccggtactgt tcgcggcggc cgcggaagtc accttcgagt gcggctgcga cgcccggata
     6661 atcgaggcgc gccttgctgc gaatcgccgc ggccgaatag ccgatgtcga cgatgtcgcc
     6721 gttcggcatg tagtcgagcc gcacgaccat cgcgcagcga tcgacgagcg ggttcagcga
     6781 gcagatgccc gccgacagct ccttcggcag catcggaatc acgcgatcgg ggaggtagac
     6841 cgagacgccg cggatggctg cttcgcgatc gagcggatcg ttccaccgca cgtagtgcga
     6901 gacgtccgcg acggcgaccc atacacgtgg accgccgtgc ggaccgtcct cgatgcacag
     6961 tgcatcgtcg aagtcgcgcg cggtctccgg atcgatcgtg cagaagcggc gatcgcgaag
     7021 atcgatgcga tcggcgaggt ccgcgggcgt cagctcctgt gacgtcgcct tcgcttgcgc
     7081 gagcacgtcg tcggggaact cgagtgggat gaccgcgcag gcgaggatct tctcgatctc
     7141 ggtgcgcgga tcctcgggat cgccgaggac cttgagcacg ttgccgccga gctccttgtt
     7201 gtaggcgtcg ggatagcgcg tgatctcgac gacgatcgcg tcgccgtcct taccgtacgg
     7261 cgcgtcgtag atcacggcgc ggccgaagtc ggcggcgatg cgcggatcat cgggctcgag
     7321 atagatcgcg cgtcctgcgc gccgcaggat gccggtgatg cgcgcgcgac cgcgcttgag
     7381 cacatcgacg acacggcctt cagtgccgcg cacgccgggc cacgtctcga ctgcgacctg
     7441 gtcgccgtcc atcgacgtgc cgcgatactt cgcgggcacg aacacggtgc cttcctcggt
     7501 cgcgacgaag ccatagcccg ccggatgcac ggtgatgcgt cctacgaggc ctccggcctc
     7561 aggcctcagg cctcgggctt ccggaatgcg cgtctctgca gttccttcgg ggaccttccc
     7621 cgtgcgttcc gctgccggtg gcttcgagac cagatcttca cgtgggggaa ggtcgacaac
     7681 gcggatctgc cccgtctcgg gatcgtattc ctcacgaggc gcgggcgacg ccttcggcgg
     7741 tacgacgcgc aacgcgccct gtttccaagg cagcttcgct tctggtttgc ggcgcgattc
     7801 cttcgcggct tttgtcggct tcatcttctt ctctggctga ggcctggggc ctgaggcctg
     7861 gggcccgcca ggcactggct tcgcgtgcgg atcggctttg cgaccctgcg gagatagcgc
     7921 gaatgcgccg cccggcagta cgttcacgta cccttcctcg acgagctcga ggatttgcgc
     7981 gcggagcgcg ctgtagtctt gtttgcgcag gccgagctca tcagcgagcg cggtcagttt
     8041 gatgcctgga gtgaccaggc gggacaggct gtccaaaatt gcctgtcgat cgatcggttg
     8101 cattggttgc tttcttatct tggttatcga aatgcgtgga gatgcccgtc gtcgccggcg
     8161 acatacaacg tgccgtcgct cgcgatcgcg ggtgtggtgt cgacatcagc cgcaagcgcg
     8221 agatgccagc gcagcgtgcc atcgggcgcg actgcgtaga gatgctcgtc ttgggcaccg
     8281 aacagaatga gtccgttcga cgcgatgcca ggggtggatg agatcttgtc ggcggcggcg
     8341 agcttccact gcacgcggcc actttgcgag atcgcataga gcttcttgtc gtaactgccg
     8401 acgtagatcg tgccgtcgtt gccgatcgcg gcaccgccgc gcacgtcctc gccggtgatc
     8461 acgcgccact gcagctcgcc cttcggcgat acagcgtaga tcgtgtgatc gtcgctgccg
     8521 acgtagatgg tgccgtcggc gccgatcgca ggcgtcgcat cgacgtcgcc gccggtccgc
     8581 agctcccact tcttcgtgcc gtcgggcgcg accgcataga gcgcgtcgtc ttcgctaccg
     8641 gcgtagacgg tgccgtcggt ggcgagcgcc ggcgtcgatg cgacgtgctc gctcgtcgcg
     8701 agcttccagc gcagcgtgcc gtcggcccac acggcgtgga tgccgtcgcc accgacatag
     8761 atcgtgccgt cggggccgat cgtcggaccg ccatcgacat cgcaacgcga cgactcggga
     8821 ccgaagccct tcggatcgca gtcgccgagc cgtagcttcc acttcagcga accgtccgac
     8881 ttcaccgcgt agaggtgatc gtcgtcggag ccgacgtaga tcgtgccgtc cttcgcgacg
     8941 gcaggtgtcg accacgagcg atcgccggtc ttgaagctcc acttgatcga gccctttgga
     9001 tcgatcgcga acagcgagcc ctcgtgggac gtgacgtaga tggtgccgtc gggaccgatc
     9061 gtcggcgagc cggcgacgac gcctttgacg tcgacgctcc acagctcctt cggcgcttgc
     9121 acgggcgcag gcccgccgcg gcgaccggtg tggcgcgcat cgccgccgaa catcgcgaac
     9181 gccggtggtc ccttcacagg agccgcctcg acaccgccat cacgagtcgt cggccgcacc
     9241 gattgcgact ggccgaccgg atgcggatcg acacagacac ccttctcgca gacgcgcgga
     9301 tccttgcagt cggtgtcttt gctgcacccg gtctggcgag cctgcgtacc cgagcacgcc
     9361 agctccacca ccgcgacggc cacagcggcc gccttccaaa ctctcacgcg cgagacccta
     9421 ctccaacaac gccgatgcgg ctacgccttc ggcaaatcgt tcgagaatcg ctcggcgagc
     9481 ttctcgaaat aacggcgggt ttcttcgagc acgaccggat cttgatccgg gtacatgcgg
     9541 atgccatggg ccccaaacgg gtcggtcgtc accgggccaa cgggaagccc acccacgatg
     9601 cccgtctgct cgtgatagag caacgcgagc acctccgagt gattctcggg caccagaaag
     9661 gtcctgcgcc cgagctcagg ttttccgtct ttaaagccgt cgaccagctt ggccatgtcc
     9721 gacgaagcca gtctaccgtt gcggcttgca tagggaaagg tccaactggc cgctcggtga
     9781 tcacggccgc ctcccacacg tgaacaccgc ttccccgggg cacatgaaga ctcggttagt
     9841 ctcctcaccg tgcgactcga gctgtggttc gacttttcgt gcccgtatgc ctatctcgcg
     9901 tcgcggcggg ccccgcacgt cgcccgggcc gcgggcgtgg agcttgatta caagcccatg
     9961 ctgctcggcg gcgtgtttcg cgggatcggc gcgggtgacg ggcctatggc cacacttggc
    10021 cccgccaaag
//
LOCUS       DASUFX010000130        18100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_21031,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000130 DASUFX010000000
VERSION     DASUFX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18100
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21031"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFV99_05000"
     CDS             complement(<1..257)
                     /locus_tag="VFV99_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058694.1"
                     /translation="MSLAQRFSVFVDAGLLQRVPTRWQLLQGEIEMTPYVISTDATAE
                     EGYRPHAFSHPLVRQLLIFSHVGRDHLRTGSALGAKLESICA"
     gene            complement(266..805)
                     /locus_tag="VFV99_05005"
     CDS             complement(266..805)
                     /locus_tag="VFV99_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058695.1"
                     /translation="MAAKKAKAKAKPTTKPAAKGKAAAKGKAAAKGKLAAKGKAAAKG
                     KAAAKGKAAAKSKAATKGTVTAKPKAKTVKAAPQSKTKASPKPKARPTEQASLNTVAV
                     ADPPKPQMELFPVDDVAPAKKGPLARLAGGVGSLIARMTGKKPQDVEQRSPDATIELA
                     TGDILMESAVPPPLPKPKR"
     gene            complement(901..1353)
                     /locus_tag="VFV99_05010"
     CDS             complement(901..1353)
                     /locus_tag="VFV99_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5058696.1"
                     /translation="MTDPIRNLSHDHADLNQRVVAIASLLRTQAPDPETHDELVLALG
                     HLREDLFLHFAREEEAVFPFIATTVPELAAQIDAMATAHDTICGALARACHLAENDAA
                     VDAIAPVFERFQVVYADHASAESILLRSLDRKLQPAQRDELAALVAGI"
     gene            complement(1353..2315)
                     /locus_tag="VFV99_05015"
     CDS             complement(1353..2315)
                     /locus_tag="VFV99_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5058697.1"
                     /translation="MSDYLVAHGHPLDRVLDAMGVDAETLADRDGRIPRRAWLHAFTV
                     AVEVTGDPALGLRVGESIRPAHLGAVGYILMSCESMRQAAELDQRWHSLIADGERLEY
                     TRDGTLSKRTLFLPDGEPPPPSCAVDCAAAAAVGFVRWLAGADDGLRRVALPYPAPAH
                     RSEHDQLFRCEVVFGATHIMVWRDPTVLRKPLAQADPELRARMEERAARLAAERSAPD
                     ELVTKVRELVARGMRDALPDLERAATVLELSPRTLKRRLADRGTSFSKIVDDTRRQLA
                     LGYIADASLSLVDVAYFCGFSEQSAFNRAFKRWTGLTPGEYRRR"
     gene            2446..3609
                     /locus_tag="VFV99_05020"
     CDS             2446..3609
                     /locus_tag="VFV99_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEX5058698.1"
                     /translation="MRIALCIVVAALTACSTDPNTGDDDIPAGCGNGTLDTGEQCDDG
                     NNNQFDGCRPDCTAVDPLMPTAMTWQYFEIPGTKCLGGTPAGFSVNYNPASTKLAIYL
                     EGGGACFNQYCESLFTPGPNQPGNGGIFDRTNAANPVKDWTWVYVPYCSGDVYAGQAD
                     TMVGGKMRAFYGYSNVTAFLERLVPSFHPDQVLLTGSSAGGFGAAVNYPQTQRAFGDV
                     PVTLIDDSGPPMSAMVFPPCLQTLWKTTWGLDKTFMKECGNDCPDATNYIADTFEHMR
                     KEFPNMNGGLFSSTGDQTIRTFAGYGWSGGYNMCKDIPNAVTAAVFTQGLDEIRTKAM
                     AAGPNFGTYYITGSSHTILRTTSFYSTTVGGKTIPQWIQGTLDGATSHVGPSN"
     gene            complement(3606..4808)
                     /locus_tag="VFV99_05025"
     CDS             complement(3606..4808)
                     /locus_tag="VFV99_05025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058699.1"
                     /translation="MPSPSRFGAYTIREPLGAGGMASVDLADWTPLEGPQRRVALKRL
                     FPHIAANPDLVAMFIDEARLARYLKHPNIAQVFEFGRIGGTYFIAFEFVQGPTVQQLA
                     RQCDLHVGYMPVPIVLEIGIQICDALHHAHNLCDEEGLPLGIVHRDISPQNLIVSTAG
                     FVKLIDFGLAKAKMSSVESQSGIIKGKLSYIAPEYLDGKLDVRCDLWAVGVVLHELLT
                     GRKLFEAPDDFKMIERVRKMAIPPASRSRAEVLLPFDDILYKALERDPRKRWQNAAAL
                     RAALVAHAKEYPPVTRSQLVAWVEWAFGQNTKARQDSGLSALHEIIESKQVVPVDEHD
                     EVSALERLPASSAAAAARQRESVAAMRIGDAMLSRRRDAPLWPWIALVVVAVILIGLA
                     ALKHSGKI"
     gene            4851..7685
                     /locus_tag="VFV99_05030"
     CDS             4851..7685
                     /locus_tag="VFV99_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5058700.1"
                     /translation="MRFIVVIALLLSARVFAQPANQEIQALEATLHKQEAANDRPGQE
                     KTLRKLVAAERSAFGEDSYQVWRREQSLLSVFMQQFEHSEAIALEKSMLAKAERVHGK
                     ESREVRDVLGDMVGSLEMARMYGEMDPVFQRLLAVSKKLNGERSSLYAYDLMRYGNSL
                     SSRAEYVAAQRVLEQSLAIMDALKENPASILTTLGLIYMQTDQAKAKAIFDRRLADDA
                     KLPTLQRAQSIWWVASIYRQRDRLDLAQPLEAQAAAILRTEIQRIEKANGKEAPDLGM
                     PLFSLGSMLMEVGDLAAADPLLTRAVALQEKLKAPFIPYAQIATLRRKQGRPKEALAL
                     YAKAQATMPGGTGLYSMMGDIERELGNTRHAEELYLAAQKDLDKLFGKHAMLVLRLHL
                     GLHGVYVAAKQLDKAERALNEELDIGEHELAFVLASGTEADHLAYFGRQASVLDTTIS
                     FHSLVAPTRASAARLALTTILRRKGRILDAAAASLGTLRTRLSPDDQKLLVELDSARA
                     KLAKVAVGGAQMTPDFAKQVAALSDQIQKLEVTLAKKNADLKVAFQTVTLADVQKKIP
                     KGARLVELVNYQPTDVTAPFSASPKLPPRRYAAYVLADHGDPTLVDLGEAAAIDDATA
                     KLRSALADPDNDRVADVAKTLHDLTFGKLTKALGNAKHVLIAPDGALNVVPFAALNDG
                     KDYLVTKYAFTYLTSGRDLLRFGVKANAKSSGPVIFADPDFDGAKTAQPAPTRRSRAM
                     QGLTWRRLPGTAQEADALAKSLAGATVYREKQATEATLKSLHAPSIVHLATHGFFLSD
                     ADASVENPLLRSGLVFAGANARSSGADDGVVTALEAAGLDLRGTRLVVMSACETGVGK
                     ITNGDGVYGLRRAFVIAGAESLVMSLWEVDDAATRDLMDGYYKKLKAGLGRSAALQAI
                     QQEMHANAKYAHPYYWASFVAAGDSAPIGK"
     gene            7719..9377
                     /locus_tag="VFV99_05035"
     CDS             7719..9377
                     /locus_tag="VFV99_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058701.1"
                     /translation="MGSRWCIVAALAASACTIDEPMPPPADLGTFPYHPLVFELDLAI
                     LAYQVHAQSMVWPIDPFFEEHAGDRGTPRDVFMTRVHEWAARRGPAQVDAMVGLDGYR
                     GPGVLAGIADNPTLDPILYDYARIHPWSDTISNNDGRWTEYLTPDAITRRIRDVYVSA
                     RPIGSDGSTVVLTQVTSPRVDYDADATDMLCAFEGGTGDRGEPGQPASYSLMGFVLLR
                     DTPGGGYDIHINFRGSRSGSAGRAVGEALDSDEAKGNPDWVTDLGWFQIGAKDAGDVT
                     SVGGVHRGIAHAMRLILPGAFQCLAKAAELRGNASPTNIYITGHSLGGGLAQHFASAV
                     LLGNQYGPRGNGPAMPDALRAWPWQHLKLVTFSAPRAGDYTWAKALSGDALDSPFYDP
                     GPIETVDADARLLLDPGIVAALHDASRPAAFRILISTDPITTTKFGGDGTHVGTTVYV
                     NGTSFIDWVGVDDPASHEPVNVRADMLGQQKDPRTPKLAWRYRPIEDFVPDRKTSEMG
                     TQAEMEKLATGLRTYYVDHDIYFDDSAFVRDLDLMFAIERGDAN"
     gene            complement(9380..10150)
                     /locus_tag="VFV99_05040"
     CDS             complement(9380..10150)
                     /locus_tag="VFV99_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058702.1"
                     /translation="MGTRPLVIVCALLATAGVAQAGDIPDASLRAVTDHVVRLQRDNA
                     PVLEGRLLAFEAATITIALSTTKEVVSVQREHVTHVIMVDEVAPATELAQPARTRMIG
                     LQMSLLGTLAVDIDYKWLHAFASTSLLLPIATAEGGGSAWYAGAAGAGVSVPMGKTSR
                     WKLDVFGELLPFHTTSFYTYLGIGTGAGFHYTAASGFTIGFTMPVIGFSARLGSSPYG
                     YDAPFRYNDSLKYFYASGLAGMPIVTLGYRFATKCRRN"
     gene            10195..10656
                     /locus_tag="VFV99_05045"
     CDS             10195..10656
                     /locus_tag="VFV99_05045"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5058703.1"
                     /translation="MIQVREPTAADDAAIATVIASGIATLRQTYRPTPAAVARKADLV
                     LHRLVAIDGDAIVGTVEYACNGERLHLMGLYTLDSHRGRGVARRVIDALVVIANAHGA
                     RTLSLYTIRETGNVPIFERLGFAVIREEAARDIASDRHPYLTEAYLERWLG"
     gene            10826..11107
                     /locus_tag="VFV99_05050"
     CDS             10826..11107
                     /locus_tag="VFV99_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058704.1"
                     /translation="MRLALLAVVALLFGFTGDARAGGNVDWSDYIDKDGSSKLPPSKT
                     PVVADSSDDAAPPPAAKATKSARTSKAKTSKKSKAKARSKSKTKAKKKH"
     gene            11139..11684
                     /locus_tag="VFV99_05055"
     CDS             11139..11684
                     /locus_tag="VFV99_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058705.1"
                     /translation="MPESRYLILLAGIVGVLAMFQPMIVIGRGPLKVKTSAYELSFGL
                     DRVHKAINKKLPGFVEKRIPPDVLQTRDDIKLVADASRGAALAYLPSLIILLLGAFSV
                     WRKRTDLAVAIAAIPLALVSIAAWFGVRYGVAYGREEEPALARLHLELQWASHVMLVV
                     GIVVLVAVGRDLLERRRTAKT"
     gene            11723..12838
                     /locus_tag="VFV99_05060"
     CDS             11723..12838
                     /locus_tag="VFV99_05060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5058706.1"
                     /translation="MKLAVLAVAVLGCSSSEPSWEWPESTPAEQGMDATVLEGARTYA
                     FQPGKNTQGVVVTRNGVLVAEWYEDGRDATSYGASWSMAKSFTSAVVGIAIDEGKIPN
                     VSKKLVDYYPQWAGSPRDEISIEHVLHQSTGLMWDEDYVIEHATTSDVVHLVFATDSP
                     LDYVLGKPLEFPPDTMFEYSSGNTLLLSGVIAKATGMRAGDYAQKKLFSKLGIKDAEW
                     WRAKTGETLTYCCLDMTSRDYARFGLLFMQHGMWDGEQIIPAAWVDASVSSAPTYAGY
                     GYQWWLEGKTDPDLPPDTFAAEGHDGQFIYVIPSLGLVVVRNGHYDKFDGPPIADPQL
                     FLRYPSDGLVEGGGTLPPDSWSAAEFLRPIISSITSP"
     gene            12889..14673
                     /locus_tag="VFV99_05065"
     CDS             12889..14673
                     /locus_tag="VFV99_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058707.1"
                     /translation="MGAHVLVVDDDPQIRRILGELLTKRGFGVDVAEDGEEGYAKALA
                     RSPDLLVTDIMMPKLDGWELVRMLRLEPTLANLPVIFLTALSTDDSRLQSFRLGPDDY
                     VNKPFKFNDLVARIEKVLGAKVGVDRKKSSGLAGDLAQVGLSTLLVLIEMERKTGVLQ
                     LTADDGRTGSISVRNGKVIDASLEHLDQLDGADAVYVMLTFTAGRFEFSVRLVDIEDR
                     IEQSTTHLLMEGARLMDEADAPDAVEQLDENAIGDWGVAERTPVVAAAKLADTVRRRA
                     RKTVPPYSPERPSSPNLRPSSPNLRPSDPKLRPSGPKLRPSGPNLRPSGSNLRPSNSN
                     LRPSGSSLRPSGSNLESAKPSRAARRASPRLLVACAVVATAGAATFMISPKPTASPTL
                     DLHGDAIAIGAALDRGVAAAQARADGIAANPDGKVEAASGEEIEVFQLRNGTLAATLR
                     VPLTAREFAPQPTPRVRVDEGIVEVVASAPSKNQQGETTGTVAVSLAVDLKDVAAPLA
                     RHTQGAKLVGLDQPVILVPGGTSAATSVTVPITSKAVTSLALEARVATTAAGPSWVAA
                     TRYAAFALAGILLLLYVGFAVRAASSRD"
     gene            14701..15585
                     /locus_tag="VFV99_05070"
     CDS             14701..15585
                     /locus_tag="VFV99_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058708.1"
                     /translation="MRALPWFLAILIGGCHPSATVEHTMPVANLQTYRTVALRVQSTA
                     FAAQGLAAYMETAVLNKLHEKCGFEHAGRPGAQPADVVLDLNITNHGRGGGGWMQNSG
                     TATIDTLLVLTDGMNGELLGTARIHGKSSGMIINGAPPENEAIEVVAKTVAELLAKSG
                     CSGPRLARAEPPPPPPNTGSGAGSGSAEQQGSGSGSGSGTPPIDDKKRADAEALNEQG
                     KEKLRTADIAGALVAFQQAVALVPDGRYQYNVCLTLEAQERWDDAINACKQARGMTSD
                     ARLVAKIDHRLDLIAHHQ"
     gene            complement(15579..15938)
                     /locus_tag="VFV99_05075"
     CDS             complement(15579..15938)
                     /locus_tag="VFV99_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058709.1"
                     /translation="MRTICIASFLLVITGCTSDTLDNTTYRRLVDQFDTESQCLETGN
                     FTPCYQTLTFCADGRVNANLDYRQEGDYTVRDSRAIARLPAVTAVFDLETLSSPQLPG
                     RHRWEQVEPLSIDCAGY"
     gene            complement(16070..16225)
                     /locus_tag="VFV99_05080"
     CDS             complement(16070..16225)
                     /locus_tag="VFV99_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058710.1"
                     /translation="MTGLSSRRARAAPCTKYADTPREVSELVEQSAGGFGYHSQEGGL
                     NNNGNDN"
     gene            complement(16222..17367)
                     /locus_tag="VFV99_05085"
     CDS             complement(16222..17367)
                     /locus_tag="VFV99_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058711.1"
                     /translation="MNVLDVFGDFNNPATGTRSWRTILTFQTGHGANGASPAAYALDV
                     TDPTAPVVVWEYTKPTSPGVKDFGTGLTVAAGPVLLNGQRTNVVVLETNNGGSGTTGV
                     VWTALSHETGAELWQRGYQYPSPPRGNSADNPLPTTGIPGGAVTADLAGSGYVTDFVF
                     GDLYGNLWLVAASTGLTRAANDESTPLFSFSTNKHPIGAPPAIYSNGSKQFAAFVSGG
                     YADPTANSWAATTQYIIAAKLDTSATSTVTEATTACMQASCALAVNTTLGNVGATGDK
                     GFAQALVVGNQLFITTDSTDVNSSSYGTTSNTGHLTAINLATGTATTAVVINSGGTSL
                     VNSSTTLFSASATKQQQTATSATSTTGTRVDLTTATLLKRALWLRTE"
     gene            complement(17342..>18100)
                     /locus_tag="VFV99_05090"
     CDS             complement(17342..>18100)
                     /locus_tag="VFV99_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5058712.1"
                     /translation="GVEALQVTWNEGALATLISADIMKKYVALAERPGKVARNDGDAS
                     ATLTSATRKVDRVYEAPFLAHATMEPMNCTADVKAGSCDVYVPTQGQTASHQAAIAAS
                     GLSADKVKIHTTYMGGGFGRRGEGDFVTEAVETSKAVGSADRVDLAGLGHHHGELAND
                     HGQDSVGLARRRRSFDRRRDRHEHTRRREPADDGVLWCRRRHASRGVRQRDRRMQRPL
                     RAGAMGVHPARPAASYPHQRATDRRIGERPGRVR"
BASE COUNT         3101 a         6026 c         5944 g         3029 t
ORIGIN      
        1 gcacagatcg attcgagctt cgcgccgagt gcgctgcccg tgcgcaggtg atcgcgaccg
       61 acgtgactga agatcaagag ctggcgcacg agcggatgac tgaacgcgtg cgggcgatag
      121 ccttcctcgg cggtggcgtc ggtcgagatc acgtacggcg tcatctcgat ctcgccctgg
      181 agcaactgcc agcgcgtcgg gacacgctgc aggagtcccg catcgacgaa caccgagaag
      241 cgctgcgcta gcgacatcgc gagagctacc gcttcggctt tggaagcggc ggcggtaccg
      301 cactctccat caagatgtca ccggtcgcga gctcgatcgt cgcatcggga gatcgctgct
      361 cgacgtcctg aggtttcttg ccggtcatcc gcgcgatcaa gcttccgaca ccgcccgcca
      421 gccgcgcgag cggacctttc ttcgcgggtg ccacgtcgtc gacggggaag agctccatct
      481 gcggcttcgg cggatctgcg acggcgaccg tgttgaggct ggcctgctcg gtcggtcttg
      541 ctttgggctt cggcgacgct ttggttttcg attgcggcgc agccttgacg gtcttggcct
      601 tgggtttcgc ggtgaccgtg cctttcgtcg cggctttgct cttggccgca gccttgcctt
      661 tggccgcagc cttgcctttg gccgcagcct tgcccttggc cgcaagcttc cctttggctg
      721 cagccttacc cttggccgca gccttgccct tggccgcggg ctttgtggtc ggctttgcct
      781 tcgctttcgc tttcttcgca gccatgggcc gaagctaacg cgatttcggg tcacgcggcg
      841 cgcctcgagt agcagtgggt tgttggcgca tggctgacgc ggctcaaacc tgagcgctcg
      901 ctagattccc gccaccaagg ccgcgagctc atctcgctgc gccggctgca atttgcgatc
      961 gagcgaccgc agaaggatcg attcagcgct cgcgtgatcc gcgtacacga cttgaaatcg
     1021 ctcgaacacc ggcgcgatcg catcgaccgc ggcgtcgttc tcggcaagat gacacgcacg
     1081 cgcgagcgca ccgcagatgg tgtcgtgggc ggtcgccatc gcgtcgatct gcgccgcgag
     1141 ctcgggcact gtcgtcgcaa tgaacgggaa caccgcttct tcttcgcgcg cgaagtgtag
     1201 aaacagatcc tcgcgcaaat ggccgagcgc gagcacgagc tcgtcgtgcg tctcggggtc
     1261 gggcgcttgc gtccgcaaca gtgacgcgat cgcgacgacg cgctgattga ggtcggcgtg
     1321 atcgtgggag aggttgcgaa tcgggtcggt cactagcgcc gacggtactc gcccggcgtc
     1381 aggcccgtcc agcgcttgaa cgcgcggttg aatgcgctct gctccgagaa gccacagaag
     1441 tacgcgacgt cgacgagact gagcgacgca tcggcgatgt agccgagtgc gagctggcgg
     1501 cgggtatcgt cgacgatctt cgaaaagctc gtgccgcgat cggcgagccg ccgcttgagt
     1561 gtgcgcggcg acagctcgag cacggtcgct gctcgctcga gatccggcaa cgcgtcgcgc
     1621 atgccgcgtg cgacgagctc acgaaccttg gtcacgagct cgtcgggcgc gctgcgttcg
     1681 gctgccagac gcgccgcgcg ttcctccatc cgcgcgcgca gctcgggatc cgcttgcgcg
     1741 agtggcttgc gcaataccgt cggatctcgc cacaccatga tgtgcgtcgc cccgaacacg
     1801 acctcgcagc gaaacagttg atcgtgttcg ctgcgatgcg cgggtgcggg atacggcagc
     1861 gcgacccggc gcaggccgtc gtcggcaccg gcgagccagc gcacgaaccc gactgccgcg
     1921 gcagctgcgc agtcgaccgc gcacgaaggt ggcggcggct cgccatcggg caagaacagc
     1981 gtgcgcttgg acagcgtacc gtcacgcgtg tactcgagcc gttcgccgtc ggcgatgagc
     2041 gagtgccagc gctggtcgag ctcggccgcc tgacgcatcg actcacagct catcaagatg
     2101 tagccgaccg ccccgagatg ggcgggccgg atgctctcgc cgacccgcag cccgagggcg
     2161 ggatcgcctg tgacctcgac cgcgacagtg aacgcgtgca gccacgcgcg gcgcgggatc
     2221 cggccgtcgc gatcggcgag cgtctcggcg tcgacgccca tcgcgtcgag aacccggtcg
     2281 agcgggtgcc cgtgcgcgac gaggtagtcg ctcaagatcc tgcaaaagct cgtgctgtag
     2341 gtgagcacac cgtccataca ctgcgggatt tcgggtcaat cgcggtgccg cagggtcaag
     2401 acgacctcgc accggcggcg gcagtgtacg ccgcgggagg ctcccatgcg tatcgcgttg
     2461 tgcatcgtgg ttgcggcttt gaccgcttgt tcgacggatc cgaacaccgg cgacgacgac
     2521 attcccgccg gctgcggcaa cggcacgctg gacaccggcg agcagtgcga cgacggcaac
     2581 aacaaccagt tcgacggttg ccgccccgac tgcaccgcgg tcgatccgtt gatgcccacc
     2641 gcgatgacct ggcagtactt cgagatcccg ggcaccaagt gcctcggcgg cacgcccgcg
     2701 ggcttctcgg tcaactacaa cccggcgtcg acaaagctcg cgatctatct cgaaggcggc
     2761 ggcgcgtgct tcaaccagta ctgcgagagc ttgttcacgc cgggcccgaa ccagccgggg
     2821 aacggcggca tcttcgatcg cacgaacgcg gcgaatccgg tcaaggactg gacctgggtc
     2881 tacgtgccgt actgctcggg tgacgtctac gccggccagg ccgacacgat ggtcggcggc
     2941 aagatgcgcg cgttctacgg ctactcgaac gtcacggcgt tcctcgagcg tttggtgccg
     3001 agcttccatc ccgaccaggt gctgctcacc ggttcgagcg ccggcggctt cggcgcggcg
     3061 gtcaattatc cgcagacgca gcgcgcgttc ggcgacgtac cggtcacgct gatcgacgac
     3121 tcgggcccgc cgatgtcagc catggtgttt ccgccgtgcc tgcagacgtt gtggaagacg
     3181 acgtggggcc tcgacaagac gttcatgaag gagtgcggca acgactgccc cgacgcaacg
     3241 aactacatcg ccgacacgtt cgagcacatg cgcaaagagt tcccgaacat gaacggcggc
     3301 ttgttctcga gcacgggcga ccaaacgatt cgcacgttcg ccggctacgg ctggtcgggt
     3361 ggctacaaca tgtgcaagga catcccgaac gcggtcaccg cggcggtgtt cacgcagggc
     3421 ctcgacgaga tccgtacgaa ggcgatggcg gcggggccga acttcggcac gtactacatc
     3481 accggcagca gccacacgat cctgcggacg acgagcttct attcgacgac cgtcggcggc
     3541 aagacgatcc cacagtggat ccagggcacg ctcgacggcg cgacgtcgca cgtcggtcca
     3601 agcaactaaa tcttgccgga gtgcttgagc gcggcgagtc cgatcaggat caccgcgacc
     3661 acgacgagcg cgatccacgg ccacagcggt gcgtcgcgcc ggcgactcag catcgcgtcg
     3721 ccgatgcgca tcgccgcgac actctcgcgc tgacgcgcag ctgcggcagc cgacgaggcc
     3781 ggcaggcgct cgagcgccga gacctcgtcg tgctcgtcga ccgggacgac ctgtttgctc
     3841 tcgatgatct cgtggagcgc agagaggccg ctgtcctgac gtgccttggt gttctggccg
     3901 aacgcccact cgacccacgc gacgagctgt gaccgcgtga ccggcggata ctccttggcg
     3961 tgcgcgacga gtgccgcgcg cagggccgct gcgttctgcc agcgctttcg cggatcacgc
     4021 tcgagtgcct tgtagagaat atcgtcgaac ggcaacagca cctcggcgcg cgagcgagac
     4081 gccggcggga tggccatctt gcgcacgcgt tcgatcattt tgaagtcgtc gggcgcttcg
     4141 aacagcttgc ggccggtgag caactcgtgg agcacgacgc cgaccgccca tagatcgcag
     4201 cgcacgtcga gcttgccgtc gaggtactcg ggcgcgatat agctgagctt gcccttgatg
     4261 attccggact gactctcgac cgacgacatc ttcgctttcg cgagcccgaa gtcgatcagc
     4321 ttgacgaagc ctgccgtcga cacgatgagg ttttgtggcg agatgtcgcg gtggacgatg
     4381 ccgagcggga ggccctcttc gtcgcagagg ttgtgcgcgt gatgaagcgc gtcgcagatc
     4441 tggatcccga tctcgagcac gatcggcacc ggcatgtagc cgacgtgaag gtcgcactgc
     4501 cgtgcgagct gctgcacggt ggggccttgg acgaactcga acgcgatgaa gtacgtgccg
     4561 ccgatccggc cgaactcgaa cacctgggcg atgttcgggt gcttcaggta gcgagcgagc
     4621 cgcgcctcgt cgatgaacat cgcgacgaga tccggatttg cggcgatgtg cgggaacaac
     4681 cgcttgagcg cgacccggcg ctgcgggccc tcgagcggcg tccagtccgc gagatcgacc
     4741 gacgccatgc cgcctgcgcc gagtggctct cggatcgtat acgccccgaa tcgggacggc
     4801 gacggcatcg ggtaagccta actcaaatcg ctactatgtt aggaagcgac atgcgcttca
     4861 tcgtcgtgat cgccttgctg ctgagcgcgc gcgtgttcgc gcaacccgcg aatcaggaaa
     4921 ttcaggcgct cgaagcgacg ctgcacaagc aagaggccgc gaacgatcgc cccggtcagg
     4981 agaagacgct gcgcaaactt gtcgccgccg agcgatcggc gttcggcgag gactcgtacc
     5041 aggtctggcg acgcgagcag tcgctgctgt ccgtcttcat gcagcagttc gagcactccg
     5101 aagcgatcgc gctcgagaag tcgatgcttg cgaaggccga gcgcgtgcac ggcaaggaat
     5161 cgcgcgaggt gcgtgacgtg ctcggtgaca tggtcggctc gctcgagatg gcgcgtatgt
     5221 acggcgagat ggatccagtg ttccagcgcc tgctcgcggt cagcaagaag ctcaacggcg
     5281 agcgcagctc gctctatgcg tacgacctca tgcgctacgg caactctctg tcctcgcgcg
     5341 ccgagtacgt cgcggcgcag cgcgtgctcg aacaatcgct cgcgatcatg gacgcgctga
     5401 aggagaaccc cgcgagcatt ctgaccacgc tcggcctgat ctacatgcag accgatcagg
     5461 cgaaggcaaa ggccatcttc gatcgcaggc tggcagacga tgcaaagctc cccaccctgc
     5521 agcgcgcaca gtcgatctgg tgggtggcga gcatctatcg ccaacgcgac cggctcgatc
     5581 tcgcgcaacc gctcgaagcg caggcggcgg cgatcctccg caccgagatc cagcgcatcg
     5641 agaaggcgaa cggcaaggaa gcgccggacc tcggcatgcc gctgttctcg ctcggctcga
     5701 tgctgatgga ggtcggcgat ctcgcggccg ccgatccatt gctcacgcgc gccgtcgctc
     5761 tacaggagaa gctcaaggcg ccgttcattc cgtacgcgca gatcgcgacg ctgcggcgca
     5821 agcaaggccg gcccaaggag gcgctcgcgc tgtacgcgaa agcgcaggcg acgatgcccg
     5881 gcggcaccgg cctgtattcg atgatgggtg atatcgagcg cgagctgggc aacacgaggc
     5941 acgccgagga gctctacctc gcggcgcaga aggatctcga caagctgttc ggcaagcacg
     6001 cgatgctcgt cctgcgattg cacctcggcc tccacggcgt ctacgtcgcc gcgaagcagc
     6061 tcgacaaagc agagcgcgcg ctcaacgagg agctcgacat cggcgaacac gagctcgcgt
     6121 tcgtacttgc ctccggcacc gaggcggacc acctcgccta cttcgggcgg caagcgagcg
     6181 tgctcgacac cacgatcagc tttcattcgc tcgtcgcgcc cacgcgcgcg tcggcggcac
     6241 gcctcgcact caccacgatt ctgcgccgca agggacgcat cctcgacgcg gcagccgcga
     6301 gcctcgggac gctgcgcacg cggttgtcgc ccgatgatca gaagctgctc gtcgagctcg
     6361 acagcgcccg cgcgaagctc gcgaaggttg ccgtcggcgg cgcgcagatg actcctgact
     6421 tcgcgaagca agtggctgcg ctgagcgatc agatccagaa gctcgaggtc acgctcgcga
     6481 agaagaacgc cgacctcaag gtcgcgtttc agaccgtcac gcttgccgac gtgcagaaga
     6541 agattcccaa gggcgcgcgc ctcgtcgagc tcgtcaacta ccagccgacc gacgtcaccg
     6601 caccgttctc ggcctcgccg aagctaccgc cgcgccgtta tgccgcgtac gtgctcgccg
     6661 accacggtga cccgacgctc gtcgatctcg gcgaggccgc agcgatcgac gacgcgaccg
     6721 ccaagttgcg cagcgcgctc gccgatccgg acaacgatcg cgtcgccgac gtcgcgaaga
     6781 cgctgcacga cctgacgttc ggcaagctga cgaaggcact cggcaacgca aagcacgtgt
     6841 tgatcgcgcc cgacggtgcg ctcaacgtcg tgccgttcgc cgcgctcaac gacggcaagg
     6901 attatctcgt cacgaagtac gcgttcacgt acctcacgtc gggccgcgac ctgttgcgct
     6961 tcggcgtgaa ggcaaacgcg aagagctcgg gtccggtgat cttcgcggac cccgacttcg
     7021 acggcgcgaa gaccgcacag cccgcaccga cgcgccgctc gcgcgcgatg caaggtctca
     7081 cgtggcgccg gctgccgggc accgcgcagg aagcggatgc gctcgcgaag tcgctcgccg
     7141 gggccactgt ctatcgcgag aagcaagcga ccgaggccac cctcaagagc ctgcacgcgc
     7201 cgagcatcgt gcacctcgcg acgcacggct tcttccttag cgatgctgat gccagcgtcg
     7261 agaatccgct gctgcgctcc ggtctcgtgt tcgccggcgc gaatgcacgc agctccggcg
     7321 ccgacgacgg tgtcgtcacg gcgctcgaag cggcgggcct cgacctgcgc ggcacgcgcc
     7381 tcgtcgtgat gtcggcgtgc gagaccggcg tcggcaagat cacgaatggc gatggcgtgt
     7441 acggcctgcg ccgcgcgttc gtgatcgcgg gcgccgagag cctcgtgatg tcgctgtggg
     7501 aagtcgacga cgcggcgacc cgcgatctga tggacggcta ctacaagaag ttgaaggcgg
     7561 gcctcggccg cagcgcggca ttgcaggcga tccagcaaga gatgcacgcc aatgcgaagt
     7621 acgcgcaccc gtactactgg gcgagcttcg tcgccgccgg tgacagcgca ccgatcggca
     7681 agtaagctgc cttcgtgtgg catcatcgcc cgattgcggt gggatctcgc tggtgcatcg
     7741 tggccgcgct tgccgcgtcg gcgtgcacga tcgacgagcc gatgccgccg cccgccgacc
     7801 tcggcacgtt tccctatcac ccgctcgtgt tcgagctcga tctcgcgatc ctcgcgtacc
     7861 aagtgcacgc gcaatcgatg gtgtggccga tcgatccgtt cttcgaggag cacgccggcg
     7921 atcgcggcac tccacgtgat gtgttcatga cacgggtcca tgagtgggca gcgcgccgcg
     7981 gtccggcgca ggtcgacgcg atggtcggac tcgacggcta tcgcggccca ggtgtgcttg
     8041 ccggcatcgc cgacaatccg acgctcgatc cgatcctcta cgactacgca cggattcatc
     8101 cgtggagtga cacgatctcc aacaacgacg gccggtggac cgagtacctc acacccgacg
     8161 cgatcacgcg acggattcgc gacgtctacg tcagcgcacg gccgatcggc agcgacggct
     8221 cgacggtcgt gctgacccaa gtaacgtcac cgcgcgtcga ctacgacgcc gacgcgaccg
     8281 acatgttgtg cgcgttcgag ggcggcaccg gcgatcgcgg cgagcccggg cagcccgcgt
     8341 cctacagctt gatgggcttc gtgctgctgc gcgatacgcc cggcggcggc tacgacattc
     8401 acatcaactt ccgcggcagc cgcagcggct cggcggggag ggccgtgggc gaagcgctcg
     8461 atagcgacga agcgaagggc aaccctgact gggtcaccga tctcggctgg tttcagatcg
     8521 gcgcgaagga tgccggcgat gtcacgagtg tcggcggcgt gcaccgcggc atcgcgcacg
     8581 cgatgcggtt gatcttgccc ggcgcgttcc agtgcctcgc gaaggcggcc gagctgcgcg
     8641 gcaacgcgtc gccgacgaac atctacatca ccggccacag cctcggcggc ggcctcgcgc
     8701 agcacttcgc gagcgcggtg ctgctcggca atcagtatgg cccgcgcggc aacggccctg
     8761 cgatgcctga tgcgttacgc gcgtggccgt ggcagcacct caagctcgtg acgttcagcg
     8821 cgccgcgcgc cggcgactac acgtgggcga aggcgctcag cggcgacgcg ctcgactcgc
     8881 cgttctacga tccaggaccg atcgagaccg tcgatgccga cgcacgcctg ctgctcgatc
     8941 ccggcatcgt cgccgcgctc cacgacgcgt cgcgaccggc cgcctttcgc atcctgatct
     9001 cgaccgatcc gatcacgacg acaaagttcg gcggcgacgg cacccacgtc ggcacgacgg
     9061 tctacgtcaa cggcacgagc ttcatcgact gggtcggcgt ggacgatccg gcatcacacg
     9121 agcccgtgaa cgtgcgcgcc gacatgctcg gccagcagaa agatccgcgc acgccgaagc
     9181 tcgcgtggcg ctaccgaccg atcgaagact tcgttccgga tcgcaagacc tccgagatgg
     9241 gcacgcaagc cgagatggag aagctggcca ccggtctgcg cacgtactac gtcgatcacg
     9301 acatctactt cgacgactcc gcgttcgtgc gcgacctcga tctgatgttc gcgatcgagc
     9361 gcggcgacgc gaactaagtt tagttgcgcc gacacttcgt cgcgaaccgg tagccgagcg
     9421 tgacgatcgg catgccggcg agccccgacg cgtagaagta cttgagcgaa tcgttgtagc
     9481 ggaacggcgc gtcgtagccg tatggcgagc tgccgagccg cgccgagaac ccgatcaccg
     9541 gcatcgtgaa accgatcgtg aagcccgacg ctgcggtgta gtggaagccg gcgccggtgc
     9601 cgatgccgag atacgtgtag aagctcgtcg tgtgaaacgg cagcagctcg ccgaacacgt
     9661 cgagcttcca gcggctcgtc tttcccatcg gcaccgacac gcccgcgccg gccgcgcccg
     9721 cgtaccacgc ggaaccgcct ccctccgcgg tcgcgatcgg cagcaacaag ctcgtgctcg
     9781 cgaatgcgtg cagccacttg tagtcgatgt cgacggcaag cgtaccgagc aggctcatct
     9841 gcaacccgat catgcgcgtg cgcgcgggct gcgccagctc ggtcgcgggc gccacctcgt
     9901 cgaccatgat cacgtgcgtc acgtgctcgc gctgcaccga cacgacttct ttcgtcgtcg
     9961 acagcgcgat cgtgatcgtc gcggcctcga acgcgagcag ccgaccttcg aggacgggcg
    10021 cgttgtcgcg ctggagccgc acgacgtgat ccgtcacggc gcgcaagctc gcatccggaa
    10081 tgtcgcccgc ctgcgcgact cccgccgtcg cgagcagtgc gcacacgatc acgagaggcc
    10141 tggtccccac agcgtttgcc attcccgatt cgacgtcgga gagtacctca acgcatgatc
    10201 caggtgcgcg aaccaacggc cgccgatgac gctgcgatcg caaccgtgat cgcatctggc
    10261 atcgcgacgc ttcgccagac ctaccggccg acacctgccg cggtggcgcg caaagcggat
    10321 ctcgtgctcc atcgcctcgt cgcgatcgac ggcgacgcga tcgtcggcac ggtcgagtac
    10381 gcgtgcaacg gcgagcgcct ccacctgatg ggcctctaca cgctcgactc gcatcgcgga
    10441 cgcggcgtcg cgcggcgcgt gatcgacgcg ctcgtcgtga tcgcgaacgc gcacggcgca
    10501 cgcacgctct cgctctacac gatccgggag accggcaacg tcccgatctt cgagcggctc
    10561 ggctttgctg tcattcgcga ggaagccgcc cgggacatcg cgagcgatcg ccacccgtac
    10621 ctgaccgagg cgtatctcga gcgctggctg gggtagccgg tgccatgaac ggtcgcgtca
    10681 atcgggactt cggcatcttg ccggcacggc ccggtcagcc agcttccgtt ccctcaccga
    10741 cgtgacgtcg tgtgcaagca ggctgtgagg cggtgctaga ggtcgcctct gccacccaga
    10801 gggcgttggt cgccactctt cttacatgag actcgcgctg ctggccgtcg tcgcactcct
    10861 cttcggattc accggcgatg cccgcgcagg cggcaacgtc gattggagcg attacatcga
    10921 caaggatgga tcgtccaagc tcccgccgtc gaagacaccg gtcgtcgccg attcgtccga
    10981 cgatgcggcg ccgccgcccg ccgcgaaggc gacgaagagc gcgcgtactt cgaaagcgaa
    11041 gacgagcaag aagtcaaaag cgaaggcgcg ctcgaagtcg aagacgaagg ccaagaagaa
    11101 acactagctg acgcaggtgc gttcataatg aacgcgccat gcccgagtct cgttacctga
    11161 tcctgcttgc aggcatcgtc ggcgtgctcg cgatgttcca gccgatgatc gtgatcggtc
    11221 gcgggccgct caaggtgaag acctccgcgt acgagctctc gttcgggctc gatcgcgtgc
    11281 acaaggcgat caacaagaag ctccctggtt tcgtcgagaa gcgaatcccg ccggatgtgc
    11341 tgcagacgcg cgacgacatc aagctcgtcg ccgatgcctc ccgcggtgcc gccctcgcct
    11401 acctgccgag cctgatcatc ctgcttctcg gtgcgttctc ggtgtggcgg aagcgcaccg
    11461 acctcgccgt cgcgatcgcg gccatcccgc tcgcgctggt ctcgatcgcg gcctggttcg
    11521 gggttcgcta cggcgtcgcg tacggtcgcg aggaagaacc agcgcttgcg cgcctgcacc
    11581 tcgagttgca atgggcttca cacgtgatgc tggtcgtcgg aatcgtggtg ctggtcgcgg
    11641 tcggccgcga tctgctggaa cgtcgccgca cagcaaaaac ctgatgacgg cgagacggcg
    11701 gttgtggcta tcgtcgccgg ctatgaagct cgcggttctc gcagtcgccg ttctcggttg
    11761 ttcgtcgtcc gagccctcgt gggagtggcc ggaatcgacg ccggccgagc aaggcatgga
    11821 cgcgaccgtg ctcgagggcg cgcgcacgta tgcgtttcag cccggcaaga acacgcaagg
    11881 cgtcgtcgtc acccgcaacg gtgtgctcgt cgcggagtgg tacgaggatg gccgcgacgc
    11941 gacgagctac ggcgcgagct ggtcgatggc gaagagcttc acgagcgcag tcgtcggcat
    12001 cgcgatcgac gaaggcaaga ttccaaacgt cagcaagaag ctcgtcgact actacccgca
    12061 atgggcgggc tcgccgcgtg acgaaatctc gatcgagcat gtgttgcacc agtcgacggg
    12121 cctcatgtgg gacgaggact atgtgatcga gcacgcgacg acgtccgatg ttgtccacct
    12181 cgtgttcgcg accgacagcc cactcgacta cgtgctgggc aagccgctgg agtttccgcc
    12241 cgacacgatg ttcgagtatt cgagcggtaa cacgctgctg ctgtcgggcg tgatcgcgaa
    12301 agcgaccggc atgcgcgcgg gtgactacgc gcagaagaag ctgttctcca agctcggcat
    12361 caaagatgcg gagtggtggc gagcgaagac cggcgagacg ctcacatact gctgcctcga
    12421 catgacgtct cgcgactacg ctcgcttcgg tctgctcttc atgcagcacg ggatgtggga
    12481 cggcgagcag atcatccccg ccgcatgggt cgatgcgtcc gtgagctcgg cgccgacata
    12541 cgcgggatac ggttatcagt ggtggctcga gggcaagacc gatcccgacc tgccgcccga
    12601 tacgttcgcg gcagaggggc acgacggcca gttcatctac gtgatcccga gcctcgggct
    12661 cgtcgtcgtt cgcaacggtc actacgataa gttcgacggt ccgccaatcg ccgacccgca
    12721 gctgttcttg cggtacccga gcgacggtct cgtcgagggc ggcggcacac tgccaccgga
    12781 ttcgtggtcg gccgcggaat tcttgcgccc catcatctcg tcgatcacca gtccctaaag
    12841 ggcagctgag ttgcccccgc tcggcctggt gggctagcgt ggaaggcggt gggtgcgcac
    12901 gttctcgtcg tcgacgacga tccgcagatc aggcggatcc tcggcgagct cctcacaaag
    12961 cgcggcttcg gcgtcgacgt cgccgaggac ggcgaagagg gctacgcgaa agcactcgcg
    13021 cgctcgcccg atttgctcgt caccgacatc atgatgccga agctcgacgg ctgggagctc
    13081 gtgcgcatgc tgcgcctcga accgacgctc gcaaacctgc cggtcatctt cttgaccgcg
    13141 ctgtcgacag acgacagtcg cctgcaatcg ttccgactcg ggcccgatga ctacgtcaac
    13201 aagccgttca agttcaacga cctcgtcgca cggatcgaga aagtgctcgg cgcgaaggta
    13261 ggcgtcgatc gcaagaagag ctcgggcctc gccggcgatc tcgcgcaggt cggcctcagc
    13321 acgttgctcg tgctcatcga gatggagcgc aagacgggcg tgctgcaact gaccgccgac
    13381 gacgggcgca ccggctcgat ctcggtgcgc aacggcaagg tcatcgacgc gagcctcgag
    13441 cacctcgatc aactggacgg cgccgacgcc gtgtacgtga tgctgacgtt cacggccggt
    13501 cggttcgagt tctcggttcg cctcgtcgac attgaagatc gaatcgagca gtcgacgacg
    13561 catctgctca tggagggcgc tcgcctcatg gacgaggccg acgcgcccga cgctgtggaa
    13621 cagctcgacg agaatgcgat cggcgactgg ggcgtcgcgg agcgcacccc ggtggtcgct
    13681 gccgcaaagc tcgccgacac cgtgcggcgc cgcgcgcgca agacggtccc accctactcg
    13741 cccgagcgac cgtcgtcccc gaacctacgc ccgtcgtcgc ccaacctacg gccgtctgat
    13801 ccgaagctgc gaccgtcggg tcccaagcta cgaccgtcgg gcccgaacct tcgtccgtcg
    13861 ggctccaatc tccggccgtc gaattccaac cttcgaccgt cgggctcgag ccttcggccg
    13921 tcgggctcga acctggaatc cgcgaagccg tcgcgcgcag ctcgccgtgc ctcgccgcgc
    13981 ttgctcgtcg cctgcgcagt cgtcgctacc gccggtgcgg ccacgttcat gatcagcccg
    14041 aagccaaccg catcgcccac tctcgacctc catggcgatg cgatcgcgat cggtgcagca
    14101 ctcgaccgcg gcgtcgctgc ggcacaagcg cgcgccgacg ggatcgcggc gaaccccgac
    14161 ggcaaggtcg aagctgcgag cggcgaagag atcgaagtgt tccagctgcg caacggcacg
    14221 ctggcggcga cactgcgggt cccgttgact gctcgcgagt tcgcaccgca gcccacgccg
    14281 cgtgtgcgtg tcgacgaggg catcgtcgag gttgtcgcaa gcgcgccgag caagaaccag
    14341 caaggcgaga cgaccggtac ggtcgcggtc tcgctcgccg tcgatctgaa agatgtggct
    14401 gctccgctcg ctcgccacac ccagggcgcg aagctcgtcg ggctcgatca gccggtcatc
    14461 ctcgtacccg gcggcacgag cgccgcaacc agtgtcaccg tgccgatcac gagtaaggcc
    14521 gtgaccagcc tcgcactcga agcacgcgta gcaacgactg cagctggacc gagctgggtc
    14581 gccgcaacgc gctacgccgc gtttgcgctc gccgggatcc tgctgctgct ctatgtcggg
    14641 tttgccgtgc gggcggcatc gtcgcgggac tagctcgtcg cgtcgcgata tgctctggcc
    14701 gtgcgcgcac tcccctggtt cctagctatc ttgatcggcg gctgccatcc gtcggcgacc
    14761 gtcgagcaca cgatgccggt cgcgaacctt cagacgtacc gcaccgtcgc attgcgcgtg
    14821 cagtcgaccg cgttcgcggc gcagggtctc gccgcgtaca tggagaccgc ggtcctcaac
    14881 aagctgcacg agaagtgcgg cttcgagcac gcgggacgcc cgggcgcgca gcctgctgac
    14941 gtcgtgctcg atctcaacat cacgaaccac ggacgcggtg gcggtggctg gatgcagaat
    15001 tccggcacgg cgacgatcga cacgctgctc gtgctcactg atggcatgaa cggtgagcta
    15061 ctcggcacgg cgcgcattca tggcaagtcg tcgggcatga tcatcaacgg cgcgccgcca
    15121 gagaacgaag cgatcgaggt cgtcgcgaag acggtcgccg agctgctcgc gaaatccggt
    15181 tgctccggcc cgcgcctcgc acgtgccgag ccgccacctc cgccgccgaa cactggctcc
    15241 ggcgcaggct cgggcagcgc cgagcagcaa ggcagcggca gtggcagcgg cagcggaacg
    15301 ccgccgatcg acgacaagaa gcgcgccgac gccgaggcac tcaacgagca aggcaaggag
    15361 aagctgcgta ccgccgacat cgccggcgcg ctcgtcgcgt tccagcaagc ggtcgcgctc
    15421 gtgcccgacg gtcgctatca gtacaacgtc tgcctcacgc tcgaagcgca ggagcgctgg
    15481 gacgacgcga ttaacgcgtg caaacaggcg cgcgggatga cgtcagacgc gcgactcgtg
    15541 gcgaagatcg atcatcgtct cgatctgatc gcgcaccatc agtaaccggc gcagtcgatc
    15601 gacagcggct cgacctgctc ccagcgatgg cggcccggca gctgcggcga tgacagcgtc
    15661 tccagatcga acacggccgt gaccgccggc agcctcgcga tcgcgcgcga atcacgcacc
    15721 gtgtagtcgc cctcctggcg gtagtcgaga ttcgcgttga cgcgaccgtc cgcgcagaac
    15781 gtgagcgtct ggtagcacgg cgtgaagttg ccggtttcca aacactggct ctcggtgtcg
    15841 aactggtcga cgagccggcg ataggtcgtg ttgtcgagcg tgtcgctggt gcagccggtg
    15901 atcacgagca gaaacgaggc gatgcagatc gtgcgcatga cccggctagc catcgcaaac
    15961 tccacgccag caccgcggcc gctgatctcg acgggttacg cgcgcagaac cctgctcgtc
    16021 gcctcgatcg gagaggactg tctccccggt actcccgccc catgggcgct tagttgtcgt
    16081 tgccgttgtt gttgagcccg ccttcttgcg agtgatagcc gaagcctcct gccgattgct
    16141 cgaccagctc ggagacttcg cgcggcgtgt ccgcgtactt cgtgcagggg gcggcacgag
    16201 cccggcggct gcttagcccg gtcactcggt gcgcaaccac agagctcgtt tgagcagcgt
    16261 ggctgttgtc aggtcgacac gggttccggt cgtggacgtc gcgctcgtag ctgtctgttg
    16321 ctgcttggtc gccgacgcgc tgaagagcgt cgtcgacgag tttactagcg aggtcccgcc
    16381 cgaattgatc acaacggcgg tggtggcggt gccggtcgct aggttgatcg ctgtcaagtg
    16441 cccggtgttg gacgtcgtgc catagctcga cgagttgacg tcagtcgaat cggtcgtgat
    16501 gaacagctga ttgccgacga cgagcgcctg cgcaaagcct ttgtctccgg ttgcaccgac
    16561 gttgccgagc gtcgtattga ccgcaagcgc acacgacgct tgcatgcacg cagtggtggc
    16621 ctcggtgacg gtgctggtcg cgctggtatc gagcttggcg gcaatgatgt actgggtcgt
    16681 cgctgcccag gagttggcag tcgggtctgc gtagccgccc gagacgaacg ccgcaaattg
    16741 cttgctgccg ttggaataga tcgcgggcgg cgcaccgatc ggatgcttgt tcgtagagaa
    16801 cgagaacagg ggtgtgctct catcgttcgc cgcgcgcgtc agaccggtgc ttgcagcgac
    16861 gagccagaga ttcccgtaca ggtcgccaaa cacgaagtcg gtgacgtagc cggaaccggc
    16921 gagatcggcc gtgaccgctc cgccggggat gcctgtcgtg ggcagtgggt tatcggcgct
    16981 gttgccacgc ggcgggctcg ggtactggta gccgcgctgc cagagctcgg cgccggtctc
    17041 gtgcgagagc gctgtccaca caacacccgt cgtacccgag ccgccgttgt tggtttcgag
    17101 gacgacgacg ttcgtgcgct ggccgttgag caggaccgga ccggcggcaa cagttagccc
    17161 agtgccgaag tctttcacac cgggtgacgt tggcttcgtg tattcccaca cgacaacagg
    17221 cgccgtgggg tcggtgacgt ctagcgcgta ggcggctggg cttgccccgt tcgccccgtg
    17281 gccggtctgg aacgtgagga tcgtgcgcca gctgcgtgtc ccagtcgccg ggttgttgaa
    17341 gtcaccgaac acgtccagga cgttcaccga tccgtcgatc cgttgcacgt tggtgcggat
    17401 aagaggcagc tggacgcgcg ggatgaacgc ccatagctcc tgcccgaagt ggccgctgca
    17461 tgcgccggtc gcgctggcgc acaccgcgtg aagcatgccg tctgcggcac caaagtacgc
    17521 catcgtcggc cggctcgcgc cggcgagtgt gctcgtgtcg atcacggcga cggtcgaacg
    17581 atcgacgccg ccgagcgagc ccgaccgaat cttgcccatg atcgtttgcc agttcgccgt
    17641 ggtgatgccc gagacctgcg aggtcgacgc gatcagcgga cccgacggct ttcgatgtct
    17701 cgaccgcttc ggtgacgaag tcgccctcgc cgcgccggcc gaagccgcca cccatatagg
    17761 tggtgtggat cttgaccttg tccgcgctca gacccgacgc cgcgatggcc gcctgatgcg
    17821 aggccgtctg tccctgcgtc ggcacgtaca cgtcacagct cccagccttc acatccgccg
    17881 tgcagttcat cggctccatc gtcgcgtgcg cgaggaaagg agcttcgtac acccgatcca
    17941 ccttgcgcgt tgcgctggtc agtgtggcgg acgcgtcgcc gtcgttgcgc gcgaccttcc
    18001 ctggccgctc ggcgagcgcg acatacttct tcatgatgtc ggcgctgatc agcgtggcga
    18061 gagcaccttc attccatgtg acctgcagcg cttccacgcc
//
LOCUS       DASUFX010000131         4979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_298845,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000131 DASUFX010000000
VERSION     DASUFX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298845"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFV99_05095"
     CDS             <1..651
                     /locus_tag="VFV99_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX5058713.1"
                     /translation="RDLGCGMRAVIVVLALVLASRTAAAEPDNRDLVLWGLAMTPPTY
                     LIGVTLHEGSHALAAELMGGDVTEVRLFPPGVDPKVHKFRFGWTYVQGLQTKPQKIVF
                     YLAPKITDSLLLGGFAALILTNTWPDNRYAQLALTVGATGLWVDFSKDVVLFSKHNDV
                     SKAFHAWCMYGIKQLPARLVYGALVAASGYTVFRGYQKTFGEQVGTTMMPLLSARF"
     gene            765..1568
                     /locus_tag="VFV99_05100"
     CDS             765..1568
                     /locus_tag="VFV99_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5058714.1"
                     /translation="MLDLSWWQLFALVGTGVVAVFAGKLVGNWAHRAVYRRVLLTRTS
                     IDDRFVQRLEGPAECVGMVVVWQLFVSFGDYPHGVLDFCRAVGNIGLLLALGWGAMRM
                     IDTGVELLATRSRWITNQRASQALLPLARRITKIMLGVLIGVMVLARLGYSIGPLLVL
                     LAIVGAAMALASHRPLENVLAAYAILGDHGVREGDTVRLDSGIVGDIEQISLYSTRIR
                     TGEGSYVIVPNRKLADAQIERSVVRPSTRAHAAVPPPNAMRSTSPGGLS"
     gene            1565..2068
                     /locus_tag="VFV99_05105"
     CDS             1565..2068
                     /locus_tag="VFV99_05105"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5058715.1"
                     /translation="MKRACIAIVDAARARLYTYQEDAIPGREMREIRDLANAGRRMKA
                     GDMFSETRPDAAGLGRPGQGAVPNSTKDDHRDDHIEMMDAKFAKEVVEEVEKVIRTEG
                     YNHLILIAPPKMLGELRKANGLLKRNGLVVDEVQRDLSNLSATQLHDHLASLDMIPGR
                     QRLNLAR"
     gene            2314..3435
                     /locus_tag="VFV99_05110"
     CDS             2314..3435
                     /locus_tag="VFV99_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058716.1"
                     /translation="MLKTNLVLSLGLAMFAACTNDGADDDMMGDDGGVDPCVMDSSQC
                     ARPPITEKGASTLAGWSDAGYKDGNRQINLFNNPTGVAVGPDGKVYVADFDNSKLRAV
                     DSNGNSSTVIAKTGFARPFSLAFIGDTLYVGTDNDCSGMHDPGENNTQMSGAIWKVDI
                     NAKAAMCLIDRIGRPRGLAALKDGRLGVADYAHHTIQIFDPNSRTLTPLAGSFNAPGA
                     ADGAGAAATFNVPYGIVQRNDGKLVVADWANHKIRVVGLDGSVATMGGSSAGFADGGM
                     AGAKFNHPLAMSIGSNGDIYLTDTDNFRIRKISADGSSVSTVAGSSSGGFKDSENASE
                     AQFYGLEGVSVSSDGKTIYVGDGNRGENMPYNRVRIVKF"
     gene            3583..4749
                     /locus_tag="VFV99_05115"
     CDS             3583..4749
                     /locus_tag="VFV99_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5058717.1"
                     /translation="MTIERPILDAAVRMRRQRDPHLIATVVAVRGSGCRRPGARMLLT
                     QFRWLTGVATGGQLAGELAKQAWSRTRSGDPIVISYDSRAGDADDDLRSAFGLGCEGV
                     VDVMIERAGIPGRLDPLEFASACIRMQQRGAIVTVIRSHVPDIKIGSRVAVRAGGDAY
                     ADVADAILTTAMMADARAAIASGDSTNCTYNSTGGSVEVFVEAIVPPPRMFVLGTGHD
                     AVPVVQLARTLGWDVTVCTDSVSESTRRRFGHADELLVGAPAELAARIEECDRAVAVV
                     MNHDYELDRAHLAMLVKTRARYIGVLGPRGRTSRMLDELGLVAQADPRLHAPVGLELG
                     AETPQEVALAILAEVQAVLAHVPAQSLREHVGSIHERPLPRISYEIETLVTVAS"
BASE COUNT          826 a         1614 c         1636 g          903 t
ORIGIN      
        1 cgagatttgg gttgcggcat gcgcgcggtg atcgtcgtct tggcgctcgt gctcgcctcg
       61 cgcaccgcgg ccgccgagcc cgacaatcgc gacctcgtgc tgtgggggct cgcgatgaca
      121 ccgccgacgt atctcatcgg cgtcacgctg cacgaaggca gtcacgcgct cgccgccgag
      181 ctcatgggtg gcgacgtcac cgaggtgcgg ttgtttccgc cgggcgtcga tccgaaggtg
      241 cacaagttcc ggtttggctg gacgtacgtg caaggtttgc agaccaagcc gcagaagatc
      301 gttttctatc tcgcgccaaa gatcaccgac tcgctcctgc tcggtgggtt cgcggcgctc
      361 attttgacga atacgtggcc ggacaaccgg tatgcgcagc tcgcgttgac cgtcggtgcg
      421 acgggactgt gggtcgattt ctcgaaggac gtcgtgctgt tctcgaagca caacgacgtc
      481 agcaaagcgt ttcatgcctg gtgcatgtac gggatcaagc aactgcccgc gcggctcgtg
      541 tatggcgcct tagtggctgc gtcgggctat acggtgttcc gcggatacca gaaaacattc
      601 ggcgagcaag tcgggacgac gatgatgccg ctgttgtcgg cgcgcttctg agcttcgtcg
      661 cgatgccgtg cgtgggcgcg cgcgcgggtt ggctcgacga cggtgcaacg gttcgtttca
      721 catcgtcgtg agcgcgggca ccatgattgc agcctctgcg gcgcatgctg gacctcagct
      781 ggtggcagct gtttgctctc gtcggaaccg gggtggtggc ggtgttcgcg gggaagctcg
      841 tcggtaactg ggcgcaccgc gccgtgtatc gacgcgtgct gctgacgcga acatcgatcg
      901 acgacaggtt cgtgcagcgc cttgaaggcc ccgccgaatg tgtcggcatg gtcgtcgtct
      961 ggcagctgtt cgtcagcttc ggcgactacc cgcacggcgt gctcgacttc tgccgcgcgg
     1021 tcggaaacat cggcctgttg ctcgcgctcg gctggggtgc gatgcgcatg atcgacacgg
     1081 gtgtcgagct cctcgcgacg cgctcgcgtt ggatcacgaa ccagcgcgca tcgcaggcgc
     1141 tgcttccgct cgcgaggcgc attaccaaga tcatgctcgg tgtgctgatc ggtgtgatgg
     1201 tgctcgcgcg actcggctac agcatcggac cgctactcgt gctgctcgcg atcgtcggcg
     1261 ctgcgatggc gctcgcgtcg catcggccgc tcgagaatgt cctcgcggct tacgcgattc
     1321 tcggcgacca cggtgttcgc gaaggcgata ccgtgcgcct cgacagcggg atcgtcggcg
     1381 acatcgagca gatcagtctg tactcgacgc gcatccgcac cggcgaaggc tcgtacgtga
     1441 tcgtaccgaa ccgcaaatta gccgacgcgc aaatcgagcg ctcggtcgtg cgtccgtcga
     1501 cgcgcgcgca tgccgccgta ccgcctccca acgcaatgag gtcgacgtca ccaggagggc
     1561 tttcatgaaa cgtgcttgta tcgcgatcgt cgacgcggcc cgcgcgcgtc tatacacgta
     1621 ccaagaggat gcgattcccg gccgtgagat gcgcgagatc cgcgatctcg cgaacgcagg
     1681 ccggcgcatg aaggccggcg acatgttcag cgagacgcga cctgatgccg cagggctagg
     1741 gcgcccgggc cagggggcgg taccgaactc cacgaaggac gaccaccgcg acgaccacat
     1801 cgagatgatg gacgcgaagt ttgcgaagga ggtcgtcgag gaagtcgaga aggtcatacg
     1861 caccgagggt tacaatcacc tgatcctgat cgcgccgccg aagatgctcg gcgagctccg
     1921 caaggcgaac ggcctgctca agcgaaatgg cctcgtcgtc gacgaagttc agcgcgatct
     1981 gtcgaacctc agcgcgacgc agctgcacga ccacctcgcc tcgctcgata tgattccggg
     2041 gcgccaacgt ttgaatctcg cgcgctgact cgtgtggcgg cgtacatcgc tgcccatcgc
     2101 cgtcgccgag caaacgttgc ttgcgcgctg aaccacgcac gatgtgcgac tgcacgtcgt
     2161 gcgtttttgc gtgagatgag atcacgcagc gtgacctcgc gccgcgtgcg ggtccgttgc
     2221 atctcaccgc gcgtggacgc acgtagtcgt cgacgaaact ccgcgcatcc gcacgcgttt
     2281 cgcacggcaa gcttcttgct cccttggttc gccatgttga agacgaacct cgtactttct
     2341 ctggggctcg cgatgtttgc ggcctgcacc aacgacggtg cggatgacga catgatgggc
     2401 gatgacggcg gtgtcgatcc ttgcgtgatg gactccagcc agtgcgcgcg tccaccaatc
     2461 accgaaaagg gcgcgagcac gctcgcgggc tggtcagacg ctggctacaa ggacggcaac
     2521 cgccagatca atctcttcaa caacccgacc ggcgtcgcgg tcggccccga cggcaaggtc
     2581 tacgtcgccg acttcgacaa cagcaagctg cgcgcggtcg atagcaatgg caattcgagc
     2641 acggtgatcg cgaagaccgg cttcgcacgt ccgttcagcc tcgcgttcat cggcgatacg
     2701 ctctacgtcg gcaccgacaa cgactgcagc ggcatgcacg atcccggcga gaacaacacg
     2761 cagatgtcgg gtgcgatctg gaaggtcgac atcaacgcga aggctgcgat gtgcctgatc
     2821 gatcgcatcg gccggccgcg cggtctggct gcgctaaagg acggtcgcct cggcgtcgcc
     2881 gactacgcgc accacacgat ccagatcttc gatccgaatt ctcggacgct cacgccgctc
     2941 gcaggcagct tcaacgcgcc aggcgcggcg gatggtgcgg gcgcggccgc gacgttcaac
     3001 gtcccgtacg gcatcgtgca gcgcaatgat ggcaagctcg tcgtcgccga ctgggcgaac
     3061 cacaagattc gcgtcgtcgg cctcgacggt agcgtcgcga cgatgggcgg cagcagcgcg
     3121 ggcttcgccg acggcggcat ggccggcgcg aagttcaacc atccgctcgc gatgtcgatc
     3181 ggatcgaacg gcgatatcta tctgaccgac accgacaact tccgcatccg caagattagc
     3241 gctgacggct cgagcgtgtc caccgtcgcc ggtagcagca gcggtgggtt caaggacagc
     3301 gagaacgcgt ccgaggcgca gttctacggc ctcgaaggcg tgtcggtgtc gagcgatggc
     3361 aagaccatct atgtcggcga cggcaaccgc ggcgagaaca tgccgtacaa ccgcgtccgc
     3421 atcgtgaagt tctagacgcg cgggctgcag acccgctgcc tctcgttgtt cgtcgcacta
     3481 cgttcgcagt accccgttgt ccctcgatgc ggactcgcct ccgcgggctc gcatcgacca
     3541 ggcgcctctc ggcgtcctgc cacccgtttg cccgagctgt ctatgacgat cgaacgcccc
     3601 attctcgatg ccgcggtccg catgcgccgc caacgcgatc cgcacttgat cgcaaccgtg
     3661 gttgctgtgc gcggctctgg ctgccgccgt cccggcgctc gcatgctgct tactcagttt
     3721 cgttggctca ccggcgttgc gaccggtggc cagctcgctg gcgagctcgc gaagcaggcg
     3781 tggtcgcgga cgcgcagcgg tgatccgatc gtcatctcct acgactcacg tgccggcgat
     3841 gccgacgacg atctgcgctc ggcattcggc cttggctgcg agggcgtcgt cgacgtcatg
     3901 atcgagcgcg ccgggatacc cggccgtctc gatccgctcg agtttgcatc ggcgtgcatc
     3961 cgcatgcagc aacgcggcgc gatcgtcacc gtcattcgtt cgcacgtgcc cgacatcaag
     4021 atcggttccc gcgtcgcagt gcgcgccggc ggcgacgcgt atgcagatgt cgcggacgcg
     4081 atcctgacga cggcaatgat ggcggacgcg cgtgcggcga tcgcgagcgg cgattcgacg
     4141 aattgcacgt acaactcgac cggcggcagc gtcgaggtgt tcgtcgaagc gattgttccg
     4201 ccgccgcgga tgttcgtgct cggcaccggt cacgatgccg tgccggtcgt ccagctcgcg
     4261 cgcaccctcg gctgggacgt caccgtatgc acggacagcg tgagtgagtc gacgcgtcga
     4321 cgcttcggtc acgcggacga gcttctcgtc ggtgcgccgg ccgagctcgc agcacgcatc
     4381 gaggagtgtg atcgtgcggt cgccgtcgtg atgaaccacg actacgagct cgatcgcgcg
     4441 cacctcgcga tgctggtcaa gacccgtgcg cgttacatcg gcgtgcttgg tccgcgtggt
     4501 cgcacgagcc gcatgctcga cgagctcggt ctcgtcgcgc aggctgatcc gcggctgcat
     4561 gcgccggtcg gcctcgagct tggcgccgag acgccgcagg aggtcgcgct cgcgattctc
     4621 gccgaggtcc aggcggtgct cgcacacgtg cccgcgcaga gcctgcgaga gcatgtcgga
     4681 tcaattcacg agcgaccgct gcctcgcatc agctacgaga tcgagacgct cgtcacggtc
     4741 gcatcgtaga cgggcgcagg cgcgcgccgg ccgtcaggga ctcccgctgg catgcggcgt
     4801 gctcgttgta cggacatgac ccgcacgctg ctcgcgctcg ctctcctcgc tgtgatttgc
     4861 gcgtcggcgt acgggatcgc gaacgcagac gacaaaccac cagcgcagat gagcgccgcc
     4921 gacacacaga agtggctcgc gttcttcgac aagcttgtcg tcgtggtcgt cggctcgtc
//
LOCUS       DASUFX010000132        17805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_21254,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000132 DASUFX010000000
VERSION     DASUFX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21254"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFV99_05120"
     CDS             complement(<1..419)
                     /locus_tag="VFV99_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058718.1"
                     /translation="MLAASAAVVPSWSSNGMADAVRDGDVHAANPDDTIDAASPRPEP
                     QSAAPVGMPVEVIAATRDYKELQGVERSHYVLTRELARGGIGRVFEARDLRLGRQVAI
                     KELLPKNRDAARRFEREARITARLQHPSIIHVYEAGL"
     gene            504..1694
                     /locus_tag="VFV99_05125"
     CDS             504..1694
                     /locus_tag="VFV99_05125"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5058719.1"
                     /translation="MIHDAFTPEQWAHLQEQFAPPQVDSQHLCTFAFKVADCVEAVRL
                     GQQLFFEPRLSGGAGVTTCATCHAASLGNGNAAWYTDIRSLNAVSQGAMNPTPHNTLT
                     VVNIGLSERTSYGWTGVCDDRPCQTVSDIIDKIALPKAMASTPAIVANVIRSTPSYLT
                     SYERAFGYAVQADDAQVQTHVEVLFEAYMRHLVSINAPFDDFIRGDNNDAISPAAKRG
                     FALFVGRAMCAECHYGGMFMDDSFHVTGVQDAGFDGGLNGTGAFYTPSLRNVEKTAPY
                     MHKGTKMGLGDVIDFYHWGGDKLGYTGDKDPLVQPFDITPDEGKDLEAFLRALTGQPI
                     SDELRKDTHVVMHDRDLCFVNEAAAGMWCGSTCISPGSDPNNCGDCGTKCDKDQVCMG
                     TCVP"
     gene            1691..3190
                     /locus_tag="VFV99_05130"
     CDS             1691..3190
                     /locus_tag="VFV99_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058720.1"
                     /translation="MTGTWGLALVLVLVLAPMVATAQTAPSGNQQQPAPANPPPANAP
                     PADAPPANAPPANAPPSDAMPAEPAPQAPTAEELARRLEDNAEETDRELERNQREIKR
                     LQRETVSLREKVITQSWLTRYINLFVDVGAFSVGGDGSGIRPDIGHLYYPEYVGKVPA
                     VWVFMGDPLSTAINTNGEPSDTSTSREIERDTVNSEGRPSVMVNAVGLSIAREITDHT
                     SVSALAEFLPRPDHSMLDIELATVKYRPLSEKNLEFELGKVQSVLGVEYRYQDATRRK
                     GITPSLICRYTCGRPLGLRGQLNEGRLSVSASLTNGNNFQQLFEHDLELKSNSLPTAS
                     GHVQWTLPVGQGLEVGVSGAIGPQDGQDDSSVRQWHFGFDARVLDLENFDITAEYVQG
                     KQPGKTESMTECDAAPCLRYKGGYLMVDRYVTQKFIPYVRLDVRSAVHIHGADFVYES
                     HTFRTTMGAQFEITAKIFGKVEYTYNRELGRIPQFPDDVLTTSLVLKTD"
     gene            3190..3882
                     /locus_tag="VFV99_05135"
     CDS             3190..3882
                     /locus_tag="VFV99_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058721.1"
                     /translation="MRALYLVLLLALLAADSPPSGGSVSGTVLAIQNSKVVSRKQVYV
                     YLLPLGKHDARPGAGIKKQIVQKNRQFSPRVVVVPVGAQVWFPNADRDRHNVYSPTEP
                     TFDLGIYGPNAQGKDHVFEDEAEFDIYCDIHRFMSAKVKVVDSPYIVQAVNGKFSFSG
                     IAPGKYRVIAWGPNSTEVKYPKEITVTAGGSVALDRELHLQIKTPPLCHDRLDGTEYD
                     KNEYGKCPPDFD"
     gene            4037..5359
                     /locus_tag="VFV99_05140"
     CDS             4037..5359
                     /locus_tag="VFV99_05140"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5058722.1"
                     /translation="MACGDGKSATPDSNTPPVDAPNVDAPTPPLTDVDMAALGMQSPL
                     PAVPADTTNAYADNAAAARLGQMLFFDKSYSGAIAAAAADTGTNGGVGATNDTGKVSC
                     ASCHAVGSESLDDRRSQPGKVSVGTGVGTRNALGAVNSAFYAWTNWGGRFDSQWALPT
                     AVAEGGATMNSTRLQIAHMLYAKYRTEYNAVFPVALDADLDPAATNASRFPASGKPGQ
                     TAYETMAQADKDILNRIYANYGKALAAYMRTLVSRNSAFDRFMAGDKTALSVSAQRGA
                     KLFLTKGCAGCHSGPNFSDNKFHALAAPDATAPPTDLGRYTDVAGLLGATNLFSVNGA
                     FSDDTSTDKLTMLAQDNAQKGQFRTKSLRNIAQSAPYMHSGQLATLEDVVELYNQGGG
                     DPGTSGITKDSLLMPLNLDAGQKADLVEFMKSLTGEPLPVTIMIDTSK"
     gene            complement(5371..7725)
                     /locus_tag="VFV99_05145"
     CDS             complement(5371..7725)
                     /locus_tag="VFV99_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX5058723.1"
                     /translation="MATEVPKGSPGANIIAEEERLYGQVQARVAMGEEDEGPRVGASD
                     LDKDLLSLRDQISEARAEDLPPLIEQMTRLAALRARLGGGRSLPVDITSPYFAHLRLK
                     ESGKSRDVMVGKRGFIDREANVQIVDWRNAPVSRIYYRYEEGDDYEEEIAGRRVEGMV
                     EARRNVSIHKGNLRRIGSPQGTYLKGASGAWVQAVGQISPVLHGGMGKAARPVAKSGE
                     KGKLGIHHGVARADKSLPEIAALIDKEQFELITQPSSGIVVIQGGAGSGKTTVALHRV
                     AFLNFQDGKRFRAQNTLFVVPSQALVRYVAGVLPALGVSGVPVVTYTGWARTTRMRCL
                     PDAPTKYNPEPPEQVSRVKKHPVMLAVLERWVERQAEAIGSELAEISAEAVEAWNSKV
                     DRELVPRLQSLSAWTKKAQIDANVRVALEGFTKRWKKRADDVTLDWAELLTDPVFLKE
                     GFAGSDVSERDVERLTQWVRRQLAKPQPPPVDEEGKPVLDSEGKALGIDEDDPAGRFD
                     DEDDPLLLRLIQLKRGGLVQPGGEEMIWEHVAIDEAQDRSALEVKVLVEAVRAPDNDP
                     EKRSVTIAGDTAQRLVFDNNFTGWSELLAQIGQPVIVRPLKLSYRSTAEVMLLAREIL
                     GPELAPDEPLAARPGEPVELHEFGDVGEAVAFLGDALRNLMNREPTASCAVISRHPEQ
                     ADAYFEGLRRAEVPALRRVRRDEFNFQPGVDVTDVAQVKGLEFDYVVMVDVNDTSYPT
                     VNWARHLLHIGVTRAAHQLWLVSTGEPSRLIPSALRDGGRMGVG"
     gene            7903..9087
                     /locus_tag="VFV99_05150"
     CDS             7903..9087
                     /locus_tag="VFV99_05150"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058724.1"
                     /translation="MTVQEQALPLLTRLGNFELVRLLAQGGMADIYLARQVGIGQFER
                     HVAVKVLNAQRASDPESCALFLDEARLVGMLNHGNLASVHEVAAQGGVHYLAMEYVHG
                     ADLREILAQAQRGDASIAYECALTIVMQAAAGLDHAHRRCAPDGRPLHLVHRDVSLSN
                     IMVGHDGSVKLIDFGIATSAASIHRTNPGIVRGKASYMSPEQCLGDPVDLRTDVFALG
                     VVLYEVATGRRCFQGNTDFERMLAVVQGNFVLPSEHVADFPPALEQVICKALAIKADD
                     RFATASELIEALETVALLEGWTLGTHHTQRMMRGLFGTVVEPWATAQGDDTTYVCPPP
                     VPPPSALDVPQPTVVITKPRRFARGTESDCYETPPLPEPWHDDDAPTRGRRSLARFWA
                     AA"
     gene            9100..9933
                     /locus_tag="VFV99_05155"
     CDS             9100..9933
                     /locus_tag="VFV99_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058725.1"
                     /translation="MRLELRASRAVPVVLCLFAIFPICLIAFAPLVGKVIGVVLTALL
                     IALAIVLGKRKLIIDERGVTAKGVFGVKRVDWDEVDHYTYWSMDQQMMYAGSGQGALG
                     ALIIIGIVALVRAFRKDPNKGNRRFAQGQLKLIAKTGTKLPIDNRYNKVADALDLVFN
                     ELHPRLRAGTPDFAPFALGDTELRHAKKGAIGLADIQHIGAGGMSITIKKSGKRLSWV
                     SVRMKKIKNVLLFIELLAERGLVIKANAEVFMPPTVLDKLRAAASRQAALPQARVVAR
                     D"
     gene            10042..11979
                     /gene="speA"
                     /locus_tag="VFV99_05160"
     CDS             10042..11979
                     /gene="speA"
                     /locus_tag="VFV99_05160"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX5058726.1"
                     /translation="MARAKALLAHDPLRRWTSTDANDTYNIPRWGCGYFAVNDQGNLI
                     VQPRGEGNGAIDMKECIDELVARGIQLPILLRFSDILKARIELLCASFNTAIREYGYQ
                     GHYRGVYPIKVNQNRTVVEEIIEYGRPFHYGLEAGSKPELLAIMAIHQDDEALIVCNG
                     YKDEEYIETALLASKMGKTVILVVEKPTELDNIHRVSERVKIKPSLGFRARLSSRGAG
                     RWEQSGGDFSKFGLTAAEMVEAVEKLKGWGEVETLKLLHFHLGSQISAIKSVKNALRE
                     AGRLFCEMYKLGAKGLSYMDVGGGLGVDYDGSQTNFASSMNYTVQEYANDVVYAIKEI
                     CDADQVPHPNIVSESGRAVAAHHSVLVVNVLGVTEFETHVPAQIEKPVPPLVTNMWET
                     YQGVSQKNLLESYHDAVEYKDQVLQLFNLGHLSLQHRVLCENLFWAICEKVLMISRNQ
                     AHVPEELEGLEKALSDTYFCNFSMFQSVPDSWAVDQLFPICPIHRLTEEPTRRATLAD
                     ITCDSDGKIDSFIDLRDVKHVLELHPKHEGQDYYLGIFLVGAYQEILGDLHNLFGDTN
                     TVHVQLSPEGDYDVKEVVAGDTVSEVLQFVDYSPSDLLGRMRNNVEQALRKKLMTIEE
                     SRTLINRFRQGMIGYTYLDRE"
     gene            11985..12470
                     /locus_tag="VFV99_05165"
     CDS             11985..12470
                     /locus_tag="VFV99_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058727.1"
                     /translation="MMRLAAAMVVLVGCAPSERECIDLRADLDGPHGAGMPRRYWDYT
                     DHRASDRDSAELAAAVVGCKQSDRERDCTEVRRILARPFATPPSGDTGIWDPSPFEQL
                     RKMHFRDDEVWEAALEYTKDAGWTYYTPYSTEAETSNSRSRLAKLCGLQTITVSVDSP
                     R"
     gene            12509..13837
                     /locus_tag="VFV99_05170"
     CDS             12509..13837
                     /locus_tag="VFV99_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058728.1"
                     /translation="MRLAALAAFVVIGLVRPALADDRWMGWQTLPDGQSNGIEVSYRP
                     AQTNVCGKGKPWVAMSPRFRNNYTERVAGTLEAVYAETTGTRTSTTTFELAPGETKVI
                     EGGAICHNTNQSLKLAIVGFRFPDRDAEQKKIAEQKAAADKAAADKAAAQKAAADKAA
                     AEQRAADEARRKQLAEHHDQATEAGRQIGEHEARKSQAEADENERKRIAALEQEQAAQ
                     AARERVAASRKAQLSDLRRGEFSVRLPTGFERVDYASVSDNGLLFGVRAEVRGFAWWA
                     ADGERVNPSGTGVEFALAAGYAKITNNLASMDSPVETSLTNVFSRVRLWHGPLALGAA
                     VEWTRYSHADSGAPSQDDSLVSLSPEVAFGLLATKLVAVELGARIGAVGTSLGNLSFS
                     ASNDLYTAAYADVAINYIYAGATAMRYLSSSGNVGEAWNAMGAIGFRVPF"
     gene            complement(13840..14463)
                     /locus_tag="VFV99_05175"
     CDS             complement(13840..14463)
                     /locus_tag="VFV99_05175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5058729.1"
                     /translation="MIAGSFEYVIKIQPMRGEGQDRAAVFERNDGGITIALADGAGGT
                     SNGDLAAQAVLDTVQVLAGSDADWSVILQTLDRDTARLRGGQTTAIVVVLDEAKIRGA
                     SVGDSEAWLVYRDRRIEAITGNQERKPLLGSGAHVMPTSEAALGEATLLVASDGLFRT
                     AKPMDIARIAAGPDLAAAADNLVDLVRTDIGAVLDDVSIVLVRRLVV"
     gene            14489..14929
                     /locus_tag="VFV99_05180"
     CDS             14489..14929
                     /locus_tag="VFV99_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5058730.1"
                     /translation="MIRVRAATVADVAEILPRTRALNAQEDIVVDDAMLEAALHRLLG
                     DATLGAVWLIEDERPIGHAVVTYGFDLEFAGRDSFLTEFWIDESHRSRGAGRIALDLI
                     VDELRARDIRALHLGVRPENPARRLYERAGFTTSPRLLMTRLLA"
     gene            complement(14886..15887)
                     /locus_tag="VFV99_05185"
     CDS             complement(14886..15887)
                     /locus_tag="VFV99_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5058731.1"
                     /translation="MRVLRIVGAILALGLLAAVIYHLGPAAIAHQLLMVGPGFLWLLV
                     IHALGIAIAGLPWHVLLPRSARPTIWQSIASRFVAAGANAVTPVVSFAGDLVRLFWLP
                     HKSDRPAGVAAIVVDRLTYGAANVLFVLGGAIALVHTGLPPEYTRITVIGFIVLAVAV
                     GLIALLASRFRLVGRVHHLITRLRRKERDHQFGDDVDTSVQRMLRREPGQLALAFFFN
                     VLFRLAMSAQIVVAFHLLGVTLSWEQALVFAALPIVMAVAGFMVPSQIGVQEGAQALL
                     AHSFGIPETTAVAVVLLLRIRSLIGGAFVAVLIATKKSTLEANSTRAAASSGGAATS"
     gene            complement(15969..16655)
                     /locus_tag="VFV99_05190"
     CDS             complement(15969..16655)
                     /locus_tag="VFV99_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058732.1"
                     /translation="MKQVATDVWQVHGPGMRMPGGVVMPLNTAVIRLADRSLLLYSPI
                     KFDDAQVEAINAAGEVKHIVAPNLYHHLHLKAAKERWPHAIVHAPPGLAAKRSDLTID
                     DELGSASIGDLEVQLIAGAPKINETLLFHRPTGALLCGDFMFNVTEPANLRTKMVLAM
                     MGVGGKQLKQSRLWGFLTKDREAARASIDRVLGWPIATVVPVHGEPVAIDATALAPRL
                     SRSYGAAKPA"
     gene            complement(16664..17176)
                     /locus_tag="VFV99_05195"
     CDS             complement(16664..17176)
                     /locus_tag="VFV99_05195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5058733.1"
                     /translation="MMIRPAAEDDFTAIASITNHYIATTAIHFAYEPITADYLLEMWK
                     SSDRYPWFVVGDRQGAVGYAKSGVWRERTAYSWTAEVGLYLAPHACGRGLGTGLYDAL
                     LDELPRRGFRSAVAGITLPNEASVKLHQKCGFERVGTFYDAGWKLDGWHAVEFWQRRF
                     ATGPEGPTRV"
     gene            17203..17562
                     /locus_tag="VFV99_05200"
     CDS             17203..17562
                     /locus_tag="VFV99_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058734.1"
                     /translation="MTRLLVVAALLAACSKGDSGPPCDKVVDHMMDLTKQMMPGHDPE
                     SLGERKQMIEQCKSRKYSAATRRCLLDAKSFNDLATCQTRDQKTSTPTPPAPPEQPAP
                     TPQAPAPAPPAGSGSSS"
     gene            17581..>17805
                     /locus_tag="VFV99_05205"
     CDS             17581..>17805
                     /locus_tag="VFV99_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HEX5058735.1"
                     /translation="MHDAIVQVPEPHNEPIYLYSPGSAEKARLKAALADVEREVVEIP
                     CVVGGQRVTTGRIREVVMPHRHKHVVARFHA"
BASE COUNT         3245 a         5958 c         5702 g         2900 t
ORIGIN      
        1 cacaggccag cttcgtagac gtggatgatc gagggatgct gcagccgcgc tgtgatgcgc
       61 gcttcgcgtt cgaagcggcg cgccgcgtcg cgattcttcg gcagcagctc cttgatcgca
      121 acctgccgcc ccagccgcaa atctcgcgct tcgaagacgc gaccgatgcc gccgcgcgca
      181 agctcgcgcg tcagtacgta atggctgcgc tcgacgccct gcagctcttt gtagtcgcgg
      241 gtagccgcga tgacctcgac gggcataccc accggcgcag ccgattgcgg ctccggacga
      301 ggtgacgccg cgtcgatggt gtcatcgggg ttcgcggcgt gcacgtcgcc gtcacgaact
      361 gcatccgcca tgccgttcga cgaccacgat ggcacgaccg ctgcagaagc ggcaagcatg
      421 atacgttggt ggatagggtg atggagcggc gtctcctagc agtagccttc tgcgtcctcg
      481 ccggatgttg gactgacgaa ccgttgatcc acgacgcgtt cacgccggag caatgggccc
      541 atctgcagga gcaattcgcg ccgccgcaag tggactcaca gcacctctgc acgtttgcgt
      601 ttaaggtcgc cgactgcgtc gaggccgtgc gactcgggca acagttgttc ttcgagccgc
      661 ggctctcggg tggtgccggg gtgacgacct gcgcgacttg ccatgcggca tcgctcggga
      721 acggcaatgc ggcctggtac acggacatcc gctcgctgaa cgcggtgtcc cagggcgcaa
      781 tgaacccgac accacacaac acgctcacgg tggtcaacat cgggcttagc gagcgcacgt
      841 cgtacggatg gaccggcgtc tgcgacgatc gtccgtgtca gaccgtatcg gacatcatcg
      901 acaagatcgc gctgccgaag gcgatggcaa gcacgcccgc gatcgtcgcg aacgtcatca
      961 ggagcactcc tagctacctc acttcgtacg agcgggcttt cggctatgcc gtgcaagccg
     1021 acgatgcgca agtgcagacg cacgtcgagg tcctgttcga ggcgtacatg cgtcatctcg
     1081 tcagcatcaa tgcgccgttc gacgacttca tacgcggcga caataacgac gcaattagcc
     1141 cggctgcaaa gcgcggcttt gcgttgttcg tcggccgtgc gatgtgcgcc gagtgtcact
     1201 acggcggaat gttcatggac gactcgttcc acgtgaccgg cgtgcaagac gccggcttcg
     1261 atggcggact gaacggtacc ggcgcgttct acacaccaag cctgcgcaac gtcgagaaga
     1321 ccgcgccgta catgcacaag ggcacgaaga tggggctcgg tgacgtcatc gatttctatc
     1381 actggggtgg cgacaagctg gggtacaccg gcgacaagga tccgctcgtc cagccgttcg
     1441 acatcacgcc agacgagggg aaggacctag aggcgttcct gcgggcactg accgggcaac
     1501 cgatcagcga cgagctgcgc aaggacacgc acgtcgtcat gcacgaccgc gacttatgct
     1561 tcgtgaatga ggccgctgcg ggaatgtggt gcggaagcac ctgcatcagc ccaggcagcg
     1621 atccgaacaa ctgcggggac tgcggcacga agtgcgacaa agatcaggtc tgcatgggga
     1681 cgtgcgttcc atgaccggca cgtgggggct ggcgctcgtg ctcgtgctcg tgctcgcgcc
     1741 gatggtcgca actgcgcaga cggctccaag cggcaaccag cagcagccgg ctcctgccaa
     1801 ccctccgccg gccaacgctc cgccggccga tgctccgccc gcgaacgctc cgcccgccaa
     1861 cgctccgcct tccgatgcca tgcctgccga gcccgcgccg caggcgccga ccgcagagga
     1921 gctcgcgagg cgactcgaag acaacgcgga agaaaccgac cgcgagctcg aacgcaatca
     1981 acgcgagatc aagcggctcc agcgcgagac agtttcgctg cgcgagaagg tgatcacgca
     2041 gagctggctg acccgttaca tcaacctgtt cgtcgacgtc ggtgccttca gtgttggcgg
     2101 cgacgggtcc ggaattcgcc ctgacatcgg ccatctctac taccccgaat acgtcggcaa
     2161 ggttcccgcc gtgtgggtgt tcatggggga tccactctcg accgcgatca acacgaatgg
     2221 cgaaccgagc gacacgtcga catcgcgcga gatcgagcgc gacaccgtga atagcgaggg
     2281 tagaccttcg gtgatggtca acgctgtcgg actctcgatc gcacgagaga tcaccgacca
     2341 cacgtcggtc tcggcgctcg ccgagttcct gccgcgtccc gaccacagca tgctcgacat
     2401 cgagctcgcg acggtcaagt accgcccact cagcgagaaa aatctcgaat tcgagctcgg
     2461 caaggtccaa tcggtgctcg gcgtcgagta ccgctatcag gacgcgacgc gacgcaaggg
     2521 catcacgccg tcgctgatct gccgctacac atgcggacgt ccgctcggcc tgcgcggcca
     2581 gctcaacgag ggtcggctca gcgtctcggc gtcgctcacg aacggcaaca acttccagca
     2641 gctgttcgag cacgacctcg agctcaagag caactcgctg ccgaccgcgt cgggtcacgt
     2701 gcaatggacg ctgcccgtcg gtcagggcct cgaagtcggc gtctcggggg cgattggccc
     2761 gcaggatggc caggacgact cgtcggtccg gcagtggcac ttcggtttcg atgcgcgcgt
     2821 gctcgacctc gagaacttcg acatcacggc cgaatacgta cagggcaagc aacccggcaa
     2881 aacagagtcg atgaccgagt gcgatgccgc accgtgtctt cgctacaagg gcggctacct
     2941 gatggtcgat cggtacgtga cgcaaaagtt catcccgtac gtccgcttgg acgtgcgttc
     3001 tgccgtgcat atccacggcg cagacttcgt ctacgaatca cacacgttcc ggaccaccat
     3061 gggcgcccag ttcgaaatca cggcaaagat cttcggcaag gtcgagtaca cgtacaatcg
     3121 cgagctcggc cggatcccgc aattccccga cgacgtcctc accacctccc tcgtgctcaa
     3181 gaccgactga tgcgcgccct ttacctcgtt ttgcttctcg cgttgctcgc ggctgactcg
     3241 ccgccgagcg gcggtagcgt gagcggcacc gtgctcgcga tccagaacag caaggtcgtg
     3301 tcgcgcaaac aggtctacgt gtacctgctg ccgctcggca aacacgacgc gaggccgggg
     3361 gccggcatca aaaagcagat cgtgcagaag aaccggcagt tctcgccacg agtcgtcgtg
     3421 gtgccagtcg gcgcgcaggt ctggtttcca aacgccgatc gcgaccgtca caacgtgtat
     3481 tcaccgacgg agccgacgtt cgatctcggc atctacgggc cgaacgccca gggcaaggac
     3541 cacgtcttcg aagacgaggc cgagttcgac atctactgcg acatccatcg cttcatgtcg
     3601 gcgaaggtga aggtcgtcga ttcgccgtac atcgtgcagg cggtcaacgg caagttctcg
     3661 ttctcgggca tcgcgccggg caagtaccgc gtgatcgcgt ggggaccgaa cagcaccgag
     3721 gtgaagtatc cgaaggagat cactgtcacc gcgggcggct cggtcgccct cgaccgcgag
     3781 ctgcacctgc agatcaagac gccaccgctc tgccatgacc gcttggacgg taccgagtac
     3841 gacaagaacg aatacggcaa gtgcccgcct gacttcgact agcgagtacc cgatcgagct
     3901 atgcagcggc ggccggcagg ttttgcctgg gtaggcaaag actgcctgcg gaacgcgtgc
     3961 gtctgcgtgt gtgacgctgg cgctcgcctt gctactcgcc cggcccatgc gcgttgcata
     4021 tgcattggtc tttgtaatgg cgtgcgggga cggaaagtct gcgacgcccg acagcaacac
     4081 tccaccggtc gacgctccga atgtcgacgc accgacgccg cctctcaccg acgtcgatat
     4141 ggccgcgctc ggcatgcagt cgccgctgcc cgcggtaccg gccgacacga cgaacgccta
     4201 cgccgacaat gccgccgccg cgcggctcgg ccagatgctg ttcttcgaca agagctactc
     4261 gggtgcgatc gcagcggctg ccgctgacac cggcacgaac ggtggcgtcg gcgcgacgaa
     4321 cgacaccggc aaggtctcgt gcgcgtcctg tcacgcggtc ggcagcgaga gcctcgacga
     4381 tcgccgctcg cagccgggca aggtctcggt cggcaccggc gtcggtacgc gcaacgcact
     4441 cggcgccgtc aactcggcgt tctacgcgtg gacgaactgg ggcggtcgct tcgactcgca
     4501 gtgggcgctg ccgacggcgg ttgccgaagg cggagcgacg atgaacagca cgcggctcca
     4561 gatcgcgcac atgctctatg cgaagtaccg caccgagtac aacgcggtgt tcccggtggc
     4621 actcgatgcc gatctcgatc cggcggcgac gaacgcttcg cgcttcccgg caagcggcaa
     4681 gcccggccag accgcgtacg agacgatggc gcaggccgac aaggacatcc tcaaccgcat
     4741 ctacgcgaac tacggcaagg cactcgccgc gtacatgcgc acgctcgtca gtcgcaactc
     4801 ggcgttcgat cgcttcatgg ccggcgacaa gaccgcactc agcgtgagcg cgcagcgcgg
     4861 cgcgaagctg ttcttgacga agggctgcgc gggctgtcac tcgggaccga acttcagtga
     4921 caacaagttc cacgcgcttg ccgcgccgga tgcgacggca ccgccgacgg acctgggccg
     4981 ctacaccgac gtcgctggac tactcggcgc gacgaacctc ttcagcgtca acggcgcgtt
     5041 cagcgacgac acgagcaccg acaagctcac gatgctcgcg caggacaacg cgcaaaaggg
     5101 tcagttccgc acgaagagcc tgcgcaacat cgcgcagtcg gcgccgtaca tgcactcggg
     5161 tcagctcgcg acgctcgaag acgtcgtcga gctctacaat cagggcggcg gggatccagg
     5221 tacgagcggc atcaccaagg actcgctcct gatgccgctc aacctggacg cggggcagaa
     5281 ggctgacctc gtcgagttca tgaagtcgct gaccggcgag ccattgccgg tcaccatcat
     5341 gatcgacacg tcgaaatagc tcagccgacc ttagccgacg cccatgcgcc cgccgtcgcg
     5401 cagcgccgac gggatcagcc ggctcggctc gccggtcgac accagccaca gctggtgcgc
     5461 cgcgcgcgtg acgccgatgt ggagcaggtg ccgcgcccag ttgaccgtag gatagctcgt
     5521 gtcgttgacg tcgaccatca cgacgtagtc gaactcgagg cccttcacct gcgcgacatc
     5581 ggtgacgtcc acgccgggct ggaagttgaa ctcgtcgcga cgcacgcggc gcaacgccgg
     5641 cacttcagcg cggcgcagac cttcgaagta cgcgtcggcc tgctcggggt ggcgcgagat
     5701 cacggcgcac gatgcggtcg gttcgcggtt catcaagttg cgcagtgcat cgccgaggaa
     5761 cgcgaccgct tcgccgacgt cgccgaactc gtggagctcg accggttcgc cgggacgcgc
     5821 cgcgagtggc tcgtcgggtg cgagctcggg gccgagaatc tcgcgcgcga gcaacatgac
     5881 ctccgccgtc gagcgatacg acagtttcag cggccgcacg atcaccggct gaccgatctg
     5941 cgccagcagc tccgaccagc ccgtgaagtt gttgtcgaac acgagacgct gcgcggtgtc
     6001 gcccgcgatc gtgacgctgc gcttctccgg atcattgtcg ggcgcgcgca ccgcttcgac
     6061 gagcaccttg acctcgagcg cgctgcgatc ctgcgcctcg tcgatcgcga cgtgctccca
     6121 gatcatctct tcgccgcccg gttggacgag gccaccgcgc ttgagctgga tgagccgcag
     6181 cagcaacgga tcatcttcgt catcgaagcg gccggccgga tcgtcctcgt cgatgccgag
     6241 cgccttgcct tcggagtcga ggaccggctt gccctcttcg tcgaccggcg gcggctgcgg
     6301 cttggcgagc tgacggcgca cccactgcgt caggcgctcg acgtcgcgct cgctgacatc
     6361 cgagccggcg aagccttcct tcaagaacac cgggtcggtg agtagctcgg cccaatcgag
     6421 cgtgacgtcg tcggcgcgct tcttccagcg tttcgtgaag ccttcgagcg cgacccgcac
     6481 gttggcgtcg atctgtgcct tcttcgtcca cgccgacagc gactgcaggc gaggcacgag
     6541 ctcgcgatcg accttgctgt tccacgcttc gaccgcttcg gcggaaatct cggcgagctc
     6601 gctgccaatc gcctcggctt ggcgctccac ccagcgctcg aggaccgcga gcatcaccgg
     6661 gtgcttcttc acgcgcgaca cttgctcggg cggctcgggg ttgtacttcg tcggcgcgtc
     6721 gggcaagcag cgcatgcgcg tcgttcgcgc ccagccggtg tacgtcacga ccggcacgcc
     6781 cgacacgccg agcgccggca acacgccagc gacataacgc acgagtgcct gcgacggcac
     6841 gacgaacaac gtgttctgcg cgcggaaccg cttgccgtcc tggaagttca agaacgcgac
     6901 acgatggagc gcgacggtcg tcttgcccga gcccgcgccg ccttgaatca cgacgatgcc
     6961 gctcgacggc tgcgtgatca gctcgaactg ttccttgtcg atcagcgctg cgatctccgg
     7021 cagcgacttg tcggcgcgcg cgacgccgtg atgaataccg agcttgccct tctcgccgct
     7081 cttcgcgacc ggccgcgccg ccttgcccat gccgccgtgg agcaccggcg agatctggcc
     7141 gacggcctgg acccacgcgc cgctcgcgcc cttcaagtac gtgccctgcg gcgagccgat
     7201 gcgacgcagg ttgcctttgt ggatcgaaac gtttcgccgc gcctcgacca tgccttcgac
     7261 gcggcgaccg gcgatctctt cttcgtagtc gtcgccttct tcgtagcggt agtagatgcg
     7321 cgacaccggc gcgttgcgcc aatcgacgat ctgaacgttc gcttcgcgat cgatgaagcc
     7381 gcgcttgccg accatcacgt cgcggctctt gccgctttcc ttgagccgca gatgcgcaaa
     7441 atacggcgac gtgatgtcga ccggcagcga gcgaccgcca ccgagccgcg ccctcaacgc
     7501 cgcgaggcgc gtcatctgct cgatcagcgg cggcaggtct tcggcgcgtg cttcactgat
     7561 ctgatcgcgg agcgacaaca agtccttgtc gagatccgac gcgcccacgc ggggaccttc
     7621 gtcttcctcc cccatcgcga cgcgcgcctg gacctggcca tagaggcgct cctcttcggc
     7681 gatgatgttc gcccctggac tgcctttagg aacctcggtc gccatgcaga gaaacggcga
     7741 atctatccga atccttacgc ctgacaagag caaccaatgc gacgtctggc cgattgggcc
     7801 ctctagctca tgaaaggccc catggcgaag ctaagtacgc gacagaccat gccgctgaat
     7861 cagctgggaa tccgatccca ggccccgctc gtaggatgtg cgatgacggt tcaagagcag
     7921 gcgctgcctc tgctgacccg cctcggtaac ttcgagctcg tccgcctgct cgcgcagggc
     7981 ggcatggctg acatctacct cgcgcgccag gtcggcatcg gtcagttcga gcgtcacgtc
     8041 gccgtcaaag ttctcaacgc acaacgcgcg agcgatcccg aatcctgtgc gctgttcctc
     8101 gacgaagcgc ggctggtcgg catgctcaac cacggaaatc tcgcgagcgt gcacgaggtc
     8161 gccgcgcagg gtggcgtgca ctacctcgcg atggagtacg tccacggtgc cgacctccgc
     8221 gagatcctcg cgcaggcgca gcgcggcgac gcatcgatcg cctacgagtg cgcgctcacg
     8281 atcgtgatgc aggccgcggc cggtctcgat catgctcacc gccgctgcgc tcccgatggc
     8341 cgcccgctcc acctcgtgca ccgcgacgtc tcgctgtcca acatcatggt tggccacgac
     8401 ggctcggtga agctgatcga cttcggcatc gcgacgtcgg ccgcgtcgat ccaccgtacg
     8461 aaccccggca tcgtgcgcgg caaggcgagc tacatgtctc ccgagcagtg cctcggcgat
     8521 ccggtcgatc tgcgcaccga cgtgttcgcg ctcggtgtcg tgctctacga ggtcgcaacc
     8581 ggccgccgct gctttcaggg caacacggac ttcgagcgca tgctcgcggt cgtgcaaggc
     8641 aacttcgtgc tgccgagcga acacgtcgcg gacttcccgc ctgcgctcga gcaagtcatc
     8701 tgcaaggcgc tcgcgatcaa ggccgacgat cggttcgcca ctgcgtcgga gctgatcgaa
     8761 gcgctcgaga ccgtcgcgct cctcgagggc tggacgctcg gcacgcacca cacgcagcgc
     8821 atgatgcgcg ggctgttcgg caccgttgtc gaaccgtggg cgacggcaca gggcgacgac
     8881 accacgtacg tttgcccacc gccggtgccg ccgccgtcgg cgctcgacgt cccgcagccg
     8941 accgtcgtga tcacgaagcc gcgccggttt gcgcgtggca ccgagagcga ttgttacgag
     9001 acgccgccgc ttcccgagcc gtggcacgac gatgacgcac cgacgcgcgg tcgtcgatcg
     9061 ctcgcgcgat tttgggctgc agcctgatac agtctcggca tgcgtctcga gctgcgtgcg
     9121 agccgggcgg tcccggtggt gttgtgcctg ttcgcgatct ttccgatctg cctgatcgcg
     9181 ttcgcaccac tggtcggcaa ggtgatcggt gtcgtgctca cggcgctgct catcgcgctc
     9241 gcgatcgtgc tcggcaagcg caagctgatc atcgacgagc gcggcgtcac cgccaaaggc
     9301 gtgttcggcg tgaagcgcgt cgattgggac gaggtcgacc actacacgta ctggtcgatg
     9361 gatcagcaaa tgatgtacgc gggcagcggc caaggcgcgc tcggcgccct catcatcatc
     9421 ggcatcgtcg cgctcgtgcg cgcgttccgg aaggacccca acaagggcaa tcggcgattc
     9481 gcgcagggtc agctcaagct gattgccaag accggcacga agctgccgat cgacaaccgg
     9541 tacaacaagg tcgccgacgc gctcgacctg gtgttcaacg agctgcaccc gcgcctgcgc
     9601 gccggcacgc cggacttcgc gccgttcgcg ctcggcgaca ccgagctgcg ccacgccaag
     9661 aagggcgcga tcgggctcgc cgacatccag cacatcggcg ccggcggcat gagcatcacg
     9721 atcaagaaga gcggtaagcg gctgtcgtgg gtcagcgtgc ggatgaagaa gatcaagaac
     9781 gtgctcttgt tcatcgagct gctcgccgag cgcggcctcg tcatcaaggc gaacgcggag
     9841 gtattcatgc cgccgaccgt cctcgacaag ctgcgcgcgg ctgcgtcgcg gcaggcggcc
     9901 ctcccgcagg cgcgagtcgt ggcgcgcgac tgaattgtca cgcagcaggt gcaccacggt
     9961 ggcagagcgg cgccgcccgg cgtgccgtag cctcagatcc ccattgcacc atcgggcaat
    10021 tggccgtaac aacgtgctga gatggcacgc gcgaaagctc tactcgcaca cgatccgttg
    10081 cgccgctgga cgtcgacgga cgcgaacgac acctacaaca ttccgcggtg gggctgcggc
    10141 tacttcgcag tgaacgacca gggcaacctg atcgtgcagc cgcgcggtga gggcaacggc
    10201 gcgatcgaca tgaaggagtg catcgacgag ctcgtcgctc gcggcatcca gctgccgatc
    10261 ctgctccgct tcagcgacat cttgaaggcg cgcatcgagc tgctgtgcgc gtcgttcaac
    10321 acggcgattc gcgagtacgg ctatcagggc cactaccgcg gcgtctatcc gatcaaggtc
    10381 aaccagaacc gcaccgtcgt cgaggaaatc atcgagtacg ggcggccgtt ccactacggc
    10441 ctcgaggccg gcagcaagcc cgagctgctc gcgatcatgg cgatccatca ggacgatgaa
    10501 gcgctgatcg tatgcaacgg ctacaaggac gaggagtaca tcgagaccgc gctgctcgcg
    10561 tcgaagatgg gcaagacggt catcctcgtc gtcgagaagc cgaccgagct cgacaacatt
    10621 catcgcgtca gcgagcgcgt gaagatcaag ccgtcgctcg gctttcgcgc ccgcctgtcg
    10681 tcgcgcggcg ccggtcgctg ggagcaatcg ggcggcgact tctcgaagtt cggcctgacg
    10741 gccgccgaga tggtcgaggc tgtcgagaag ctcaagggct ggggcgaggt ggagacgctc
    10801 aagctgcttc acttccatct cggctcgcag atctcggcga tcaagagcgt caagaacgcg
    10861 ctccgcgaag ccggtcgctt gttctgcgag atgtacaagc tcggtgccaa gggcctctcg
    10921 tacatggatg tcggcggcgg tctcggcgtc gactacgacg gctcgcagac gaacttcgcg
    10981 tcgtcgatga actacacggt tcaggagtac gcgaacgacg ttgtctacgc gatcaaggaa
    11041 atctgcgacg ccgaccaggt tccgcacccg aacatcgtca gcgaatcggg ccgcgcggtc
    11101 gccgcgcacc actcggtgct cgtcgtcaac gtgctcggcg tgaccgagtt cgagacgcac
    11161 gtcccggcgc agatcgagaa gccggtgccg ccgctcgtca ccaacatgtg ggagacgtac
    11221 caaggcgtca gccagaagaa cctgctcgag agctatcacg acgccgtcga gtacaaggat
    11281 caggtccttc agctgttcaa cctcggccac ctgtcgctcc agcaccgcgt gctctgcgag
    11341 aacctcttct gggcgatctg cgagaaggtg ctgatgatct cgcgtaacca ggcgcacgtg
    11401 cccgaggagc tcgaaggtct cgagaaggcg ctctcggata cgtacttttg caacttctcg
    11461 atgttccagt cggtgccgga ctcgtgggcc gtcgatcagc tgttcccgat ctgcccgatc
    11521 catcgcctga ccgaggagcc gacgcggcgc gcgacgctcg ccgacatcac gtgtgactcc
    11581 gacggcaaga tcgactcgtt catcgatctg cgcgacgtca agcacgtgct cgagctgcat
    11641 ccgaagcacg aaggccagga ctactacctc ggcatcttcc tggtcggcgc gtaccaggag
    11701 atcctcggcg acctccacaa cctgttcggc gacacgaaca cggtgcacgt ccagctgtcg
    11761 cccgaaggcg actacgacgt gaaggaagtc gtcgcgggcg acacggtgag cgaagtgctc
    11821 cagttcgtcg actattcgcc gagcgacttg ctcggccgca tgcgcaacaa cgtcgagcag
    11881 gcgctgcgca agaagctgat gacgatcgaa gagagccgca cgctgatcaa tcgcttccgt
    11941 cagggcatga tcggctacac gtacctcgat cgcgagtgat cgtaatgatg cggctcgcgg
    12001 cggcgatggt ggtgttggtc gggtgtgctc cgagtgagcg cgagtgcatc gacctgcgcg
    12061 ccgatctcga tggtccgcac ggcgcgggca tgccacgacg ctactgggac tataccgacc
    12121 accgcgccag cgatcgtgac agtgctgagc tcgcggctgc ggtcgtgggc tgcaagcaga
    12181 gcgatcgcga gcgcgattgc accgaagtcc gcaggattct cgctcgacca ttcgcaacgc
    12241 cgccgagcgg agacaccggc atctgggatc cgtctccgtt cgagcagctg cgcaagatgc
    12301 acttccgcga cgacgaggtc tgggaggcgg cgctcgagta cacgaaagac gccgggtgga
    12361 cgtattacac gccgtacagc acggaagccg agacgtccaa ctcgcgcagc cgcctcgcga
    12421 agctgtgcgg cttgcagacg atcacagtct cggtcgactc gccgcgatag gactggccca
    12481 cggcgcgggc ctgcgcgatc attgcgacat gcggttggca gcgctcgctg cgttcgtcgt
    12541 gatcgggctc gtcaggccgg cgctcgccga cgaccgctgg atgggttggc agacgctgcc
    12601 cgacgggcag tccaacggca tcgaagtgtc gtaccggccc gcccagacca acgtgtgcgg
    12661 caagggcaag ccgtgggtcg cgatgagccc gcgcttccgc aacaattaca ccgagcgcgt
    12721 cgccggcacg ctcgaagccg tgtacgccga gacgaccggc acccgcacca gcacgacaac
    12781 gttcgagctc gcgccgggcg agacgaaagt catcgaaggc ggcgcgatct gtcacaacac
    12841 gaaccagtcg ctcaagctcg cgatcgtcgg cttccgcttc ccggatcgcg acgccgagca
    12901 gaagaagatc gccgaacaga aagccgccgc tgacaaggct gctgccgata aggccgccgc
    12961 gcagaaagcc gccgccgaca aagccgctgc agagcagcgt gcggccgacg aagcacggcg
    13021 caagcaactc gctgaacacc acgaccaggc gaccgaggcc ggacgccaga tcggcgagca
    13081 cgaggcccgc aagagccagg cggaggccga cgagaacgag cgcaaacgaa tcgccgcgct
    13141 cgagcaagag caggctgcac aggccgcacg cgaacgagtc gctgcatccc gcaaggcaca
    13201 gctctccgat ctgcgacgcg gcgaattcag cgtacggctg ccgaccggct tcgagcgcgt
    13261 cgattacgcg agcgtctccg acaacggcct cctgttcggc gtgcgcgccg aggtccgcgg
    13321 cttcgcgtgg tgggcggccg acggtgagcg cgtcaatccg agcggcacag gtgtcgagtt
    13381 cgcgcttgcg gccggctacg ccaagatcac gaacaacctg gcgagcatgg attcgcccgt
    13441 cgagaccagt ctgacaaacg tgttctcacg cgttcggctc tggcacggtc cgctcgcgct
    13501 gggtgccgcg gtcgagtgga cccgctactc acacgccgac agcggtgcac cgtcgcagga
    13561 tgactcgctg gtctcgctca gcccggaagt cgcgttcggc ctgctcgcga ccaagctcgt
    13621 cgccgtcgag ctcggcgcgc ggatcggcgc ggtcgggacc tcgctcggca acctctcgtt
    13681 cagtgcctcg aacgacctct acaccgctgc gtacgcggac gtcgcgatca attacatcta
    13741 cgccggcgcg acggccatgc gctatctctc gtcgagcggc aacgtcggcg aagcatggaa
    13801 cgcgatgggt gccatcgggt tccgcgtccc gttctgacgt cagacgacga gccggcgcac
    13861 gagcacgatc gagacgtcgt cgagcacggc gccgatgtcg gtgcgcacga ggtcgacgag
    13921 attgtcggcc gccgccgcga gatcggggcc tgccgcgatg cgcgcgatgt ccattggctt
    13981 cgcggtgcga aacaagccgt cactcgcgac gagcagcgtc gcctccccga gtgcggcctc
    14041 gctcgtcggc atgacatgcg cgcccgagcc gagcagcggc ttgcgctcct ggttgccggt
    14101 gatcgcttcg atgcgccgat cgcgatagac cagccacgcc tcggaatcac cgacgctcgc
    14161 gccgcggatc ttggcttcgt cgagcacgac gacaatcgcg gtcgtctggc cgccgcggag
    14221 gcgtgcggtg tcgcgatcga gcgtctgcaa gatcacggac cagtcagcgt cggagccggc
    14281 gagcacttgc accgtgtcga gcaccgcttg cgccgcaagg tcgccgttgc tcgtgccgcc
    14341 cgcgccgtcg gctagcgcga tcgtgatgcc tccgtcgttg cgctcgaaca ccgctgcccg
    14401 atcttgcccc tcgccgcgca tcggctggat cttgatcacg tactcgaaac tgccggcgat
    14461 cactgcgggc gcagtataga ctgcgcggat gattcgcgtg cgagctgcga ccgttgccga
    14521 tgtggcagag atcctgccgc gcacgcgcgc actaaatgcg caagaggaca tcgtcgttga
    14581 cgatgcaatg ctcgaggcag cgctgcatcg gttgctcggt gacgcgacgt tgggtgcggt
    14641 gtggctcatc gaagatgaac ggccgatcgg ccatgcggtg gtcacgtacg gtttcgatct
    14701 cgagtttgcc ggccgcgatt cgttcctgac cgagttctgg atcgacgagt ctcaccggtc
    14761 acgcggggcc ggcaggatcg cactcgatct gatcgtcgac gagttgcgtg cgcgcgatat
    14821 ccgggcattg catctcggcg tgcggcccga gaatccagca cgccggctct acgaacgcgc
    14881 agggttcacg acgtcgccgc gcctcctgat gacgcggctg ctcgcgtaga attcgcttcc
    14941 agcgtgctct tcttcgtcgc gatcaagacc gcgacaaacg cgccaccgat cagcgagcgg
    15001 atacgtagca gcagaacgac ggcgacggcc gtggtctctg gaatgccaaa gctgtgcgcg
    15061 agcagcgcct gtgcgccttc ctggacgccg atctgactcg gcaccatgaa gccggcgacc
    15121 gccatcacga tcggcagcgc ggcaaacacg agcgcttgct cccaggacaa ggtgacgccg
    15181 agcaggtgga acgcgaccac gatctgcgcg ctcatcgcga gccggaacag cacgttgaag
    15241 aagaacgcga gtgcaagctg gcccggctcg cggcgcagca tccgctggac gctggtgtcg
    15301 acgtcatcgc cgaactgatg atcgcgttcc ttgcggcgca gacgcgtgat caagtgatgc
    15361 acgcgaccga ccagtcggaa ccgcgatgcg agcagcgcga tgagaccgac cgcgacggcg
    15421 agcacgatga agccgatcac ggtgatgcgg gtgtattcgg gcggcagtcc cgtgtgaacg
    15481 agcgcgatcg caccaccgag cacgaacaga acgtttgcgg ctccgtacgt caggcgatcg
    15541 acgacgatcg cggcgacacc ggccggccga tccgacttgt gcggcagcca gaacaggcgc
    15601 acgagatcgc cggcaaacga gacgaccggc gtcactgcgt tcgcgcccgc cgcgacgaat
    15661 cgactcgcga tcgactgcca gatcgtcggc cgcgcagacc gcggtagcag cacgtgccac
    15721 ggcagcccgg cgatcgcgat gccgagcgca tggatcacga ggagccagag gaagcccggc
    15781 ccgaccatga gcagctgatg cgcgatcgcg gccggaccga ggtggtagat gacggccgca
    15841 agcagtccga gcgcgaggat cgcgcccacg atccgcagaa cgcgcatgcg acgacggcgc
    15901 tgcgagaact cctcaaccgg tgcgagcgct gtcgtcacac catcgagaga cgcaagcgac
    15961 gggccacgct aggcgggctt tgcggctccg tacgaccgcg aaagccgcgg tgcgagagca
    16021 gtggcatcga tcgcgacggg ctcgccgtgc actgggacca ccgtcgcgat cggccagccg
    16081 agcacgcgat cgatcgatgc acgtgctgcc tcccgatctt tcgtcagaaa gccccacaag
    16141 cggctctgct tcagctgctt gccaccgacg cccatcatcg cgagcaccat cttggtgcgc
    16201 agattcgccg gctccgtgac gttgaacatg aaatcgccgc agagcagcgc gcccgtcggg
    16261 cgatggaaca gcagcgtctc gttgatcttc ggcgcgcccg cgatgagctg gacctcgagg
    16321 tcgccgatcg aggcgctgcc gagctcgtcg tcgatcgtga gatcactgcg cttcgcggcg
    16381 agaccgggcg gcgcgtgcac gatcgcgtgt ggccagcgct ctttcgccgc tttgagatgc
    16441 aggtgatggt agaggttcgg cgcgacgatg tgtttgacct cgcctgcggc gttgatcgct
    16501 tcgacctgcg cgtcgtcgaa cttgatcggc gaataaagta gcagcgatcg atcggcgagc
    16561 cggatcactg cggtgttgag cggcatgacg accccacccg gcatccgcat ccccggtcca
    16621 tgcacctgcc acacatccgt agcgacttgc ttcacgcggc tagtcaaaca cgcgtcgggc
    16681 cttcgggccc tgtggcgaat cgtctctgcc agaactcgac ggcatgccag ccgtccagct
    16741 tccagccggc atcgtaaaac gtaccgacgc gctcgaagcc gcacttctgg tgcagcttca
    16801 cgctcgcctc gttcggcagc gtgatgccgg cgaccgccga acgaaagcca cgccgcggca
    16861 gctcgtcgag aagcgcgtcg tagagtccgg taccgagacc acggccacac gcgtgcggcg
    16921 ctagatacag gccgacctcg gccgtccagc tgtacgccgt gcgctcgcgc catacgcccg
    16981 acttcgcata gcccacggcg ccctggcgat cgccgacgac aaaccatgga tagcgatcgc
    17041 tcgacttcca catctcgagc aagtagtccg cggtaattgg ctcgtacgcg aagtggattg
    17101 cggtcgtcgc gatgtagtga ttcgtgatcg aggcaatcgc ggtgaagtcg tcctcggctg
    17161 ccgggcgaat catcatggcc ctcgtatgct accgtcgaag tgatgacgcg cttgctcgta
    17221 gtcgccgctt tactcgccgc ctgcagcaaa ggcgattcag gaccaccttg cgacaaggtc
    17281 gtcgatcaca tgatggatct gacgaagcag atgatgcccg gccacgaccc ggagtcgctc
    17341 ggcgagcgca agcagatgat cgagcagtgc aagagccgca agtactctgc cgcaacgcgg
    17401 cgatgcctgc tcgacgcgaa gtcattcaat gatctcgcga cctgccagac tcgcgatcag
    17461 aagacttcga cgcctacgcc accggcgccg cccgaacagc ctgctccgac gccgcaggca
    17521 ccggcaccag ctccgccggc cggctctggt agcagcagct aggcggtggt agagagggcc
    17581 atgcacgacg cgatcgtcca ggtccccgag ccgcacaacg agccgatcta tctctactcg
    17641 ccgggcagcg ccgagaaggc gcgcctcaag gcggcgctcg cggatgtcga gcgcgaggtg
    17701 gtggagattc cgtgcgtcgt cggtggccag cgcgtcacga ccggccgcat tcgcgaagtg
    17761 gtgatgccgc accgccacaa gcacgtggtc gcgcgcttcc acgct
//
LOCUS       DASUFX010000133         5477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_160387,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000133 DASUFX010000000
VERSION     DASUFX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160387"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFV99_05210"
     CDS             complement(<1..537)
                     /locus_tag="VFV99_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058736.1"
                     /translation="MRRVVVGSLVLSFATVARADDPRDVFGLGKKPTPQKSTPQKSTP
                     QKSTTEKTEDVRDVFGLTPKQQQAPLDCSDGTEFGCVDASDPLDEHETPYGLVQWLSG
                     KYLLSLPVADATHEQVAHYALGGSRDEAGPAFRGANGLENRWLIDGAPADGLRTGVAD
                     TRIPLTFLDGMWVQSGGFT"
     gene            complement(556..960)
                     /gene="trxA"
                     /locus_tag="VFV99_05215"
     CDS             complement(556..960)
                     /gene="trxA"
                     /locus_tag="VFV99_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5058737.1"
                     /translation="MATVEITAENFKDTIEKGGVVLLDFWAEWCGPCRAFAPVFEKAS
                     ETHVDVVFGKIDTESEQELAGAAGIQAIPTLMLFRDGILLFRESGALPPAALEELVTQ
                     AKKLDMEKVRKEIAEHQHEHAHEHGPDCDHDH"
     gene            1048..2967
                     /locus_tag="VFV99_05220"
     CDS             1048..2967
                     /locus_tag="VFV99_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058738.1"
                     /translation="MAALLVVACGDDAISVPPEANPLGTTTNILPYPSSLYEVRDGDG
                     VHLDVPVGAFPMNTVTMTAFDPAPLAKRRGWPASTTILWAVKGGADPAGFVGHGQIAD
                     SLTDASATVLLDMTTGERVLHFAEVDVNELDIFDHQAVYIRPGARLLGGHRYAVGIRK
                     SLHARDGSPLPISEGFQAVLDDKNTGHARLDAARPRLREAVAALETAGIKRTDLVVAW
                     DFTVEPDDSAIADPVAARDAALTAMGPLGANLTYTVTSDLGTVNNDPRLARRIEIDYQ
                     SPAVTGEAHAGFYRDDSGTVIAMGTMTTQAYVMIPACATFNNKAGILIYGHGIFGGLP
                     ELRNGEYARDLAQDGCLIVAGTVWTGMSNDDIPNALLALNDLNKGKGFGERIFQGIVN
                     TIALEQLLRGKFATELFIDSQGRSIVDTSRVYFLGVSMGHVLGTVFMAYDPFITRGTL
                     NVGGANWALMFERSNSWAAYGLPIKSSYDTLLDAVIMEQVLQMSLQPADGATIAGISV
                     PNTPEKHLLLQTSLNDASVPNVASFYHARSAGVDLLSTTIVTPYGLPAAVDSSTKSAI
                     VVVDEKPSQVPPETNEVFNFNTIAHENPRRRTLLQQQMRDFWSTGTISNVCTGPCDCA
                     AGACGTLRTAMYGGS"
     gene            3071..3646
                     /locus_tag="VFV99_05225"
     CDS             3071..3646
                     /locus_tag="VFV99_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5058739.1"
                     /translation="MSANHSQVVVGFDFSHSGRAALDRAIGLATRAPWHVLHFVCVID
                     PHFAFPALPTKHIDLEYANRVQEEVTAIVEQELKAKRTDMPIHFFVHARLGKPAAEIL
                     GCARDVGADLIIVGSKGLTGIERAVLGSVSEKVVREAGCSVEVARPKTYEYVPLLAIT
                     DAQQHQKYAAPHRYTYEDRRASRRPNDWPLY"
     gene            3701..4348
                     /locus_tag="VFV99_05230"
     CDS             3701..4348
                     /locus_tag="VFV99_05230"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5058740.1"
                     /translation="MAKLRITYNAPVVLTFALAAVIAFILEKTISGSVEWFAAYPHLD
                     RTQAYVGLFTHILGHANWQHLIGNFMLILLLGPILEERHGSGPLLLMILLTAFITGMA
                     NLVFSNGALMGASGIVFMMILLASMANVRGGEIPLTFIAVAVIYMGGEIIRAFREDQI
                     SQMAHLVGGIAGAAFGFIIAGKKGVAKAPKGGTAADLEKMLAPARAQTAKPKAKI"
     gene            complement(4382..5200)
                     /locus_tag="VFV99_05235"
     CDS             complement(4382..5200)
                     /locus_tag="VFV99_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5058741.1"
                     /translation="MSLRGKTLFITGASRGIGLAIALRAARDGANIAIVAKTAEPNPK
                     LPGTIFTAAKEIEAAGGKALPIACDIRMDDHVAAAVAKTVETFGGIDILVNNASAINL
                     TGTEQMDMKRYDLMHGINTRGTFLCSKLCIPHLKKAQNPHVLNLSPPLNMETKWFAPH
                     VAYTMAKFGMSMCVLGMAGEYKSAGIAFNALWPRTTIATAAVKNLLGGDQMIKASRKP
                     DIMGDAAYVIFNRPARECTGNFFIDDEVMMSTGKTDLSEYAVDPSQKLMPDFFV"
     gene            complement(5255..>5477)
                     /locus_tag="VFV99_05240"
     CDS             complement(5255..>5477)
                     /locus_tag="VFV99_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058742.1"
                     /translation="MSWPIAPTSIALLGVMIGLITGCVFVATKPGPPGMVGVGLSFWI
                     FGVGCVMGIAGAQMMAKVNRPPEPEWTVD"
BASE COUNT          950 a         1809 c         1718 g         1000 t
ORIGIN      
        1 ggtgaagccg cccgactgca cccacattcc gtcgaggaac gtgagcggaa tccgcgtgtc
       61 ggcgacgccg gtgcgcagtc cgtcggcggg cgcaccgtcg atgagccagc ggttctcgag
      121 cccgttggcg ccgcggaacg ccggaccggc ttcgtcgcgg gagccgccga gcgcgtaatg
      181 cgcgacttgc tcgtgcgtcg cgtcggcgac cggcaagctc agcagatact tccccgacag
      241 ccactgcacg aggccatacg gcgtctcgtg ttcgtcgagc ggatcgctcg cgtcgacgca
      301 accgaactcg gtgccatcgc tgcagtcgag cggcgcctgc tgctgcttcg gcgtcagacc
      361 gaaaacgtcg cgaacatctt ccgtcttctc ggtcgtcgac ttctggggcg tcgacttctg
      421 gggcgtcgac ttctggggcg ttggcttttt gccgagcccg aagacgtcgc gcggatcatc
      481 tgcgcgcgca acggtcgcga agctcaatac gagcgatccg acgacgacgc gccgcacgct
      541 gcgacggtag cacgcttagt gatcgtggtc gcaatccggg ccgtgctcgt gcgcgtgctc
      601 gtgctggtgc tcggcgatct ctttgcgcac cttctccatg tcgagcttct tggcctgcgt
      661 gaccagctct tcgagcgcgg ccggcgggag cgcgccgctc tcgcggaaca acaggatgcc
      721 gtcgcggaac agcatgagcg tcgggatcgc ctgaatgccc gcggcaccgg cgagctcttg
      781 ctcgctctcg gtgtcgatct tgccgaacac gacatcgaca tgcgtctcgg aggccttctc
      841 gaataccggc gcgaatgcac ggcacggccc gcaccactct gcccagaaat cgagcaggac
      901 gacgccgccc ttttcgatcg tgtctttgaa gttctctgcc gtgatctcga cggtagccat
      961 ttacctgcag cctggcgcgt tgccatgcaa acggcaagca agtcatccga gattccatgg
     1021 caaactggca cgatgcgtgg agtcatcgtg gctgctctgc ttgtcgtcgc gtgcggcgac
     1081 gacgcgatca gcgttccccc cgaggcaaat ccgctcggaa cgacaaccaa cattctccct
     1141 tatccttcat cgctctacga ggtccgcgac ggcgacggcg tgcacctcga tgtcccggtc
     1201 ggcgcgttcc cgatgaacac ggtgacgatg acggcgttcg acccggcgcc cctcgccaag
     1261 cggcgagggt ggccggcatc gaccacgatc ttatgggcag tgaagggcgg cgccgacccc
     1321 gccggtttcg tcggccacgg ccagattgcc gatagcctca ccgacgccag cgcgaccgta
     1381 ctgctcgaca tgacgacggg cgagcgcgtc cttcacttcg ccgaagtcga cgtcaacgag
     1441 ctcgacatct tcgatcatca ggcggtttac atccggcctg gcgcgcggct tctcggtggc
     1501 catcgctacg cggtcggcat tcgcaagtcg ctccacgcgc gcgacggtag cccgctgccg
     1561 atcagcgagg gctttcaagc cgtgctcgat gacaagaaca cgggacatgc gcgtctcgat
     1621 gctgcgcggc cgcggttgcg tgaggcggtc gccgcgctcg agaccgccgg catcaaacgt
     1681 accgatctcg tcgttgcgtg ggattttacg gtcgagcccg atgattccgc gatcgcggac
     1741 ccggtcgccg cgcgcgatgc ggcactcacc gcgatggggc cgctcggcgc gaacctcacg
     1801 tacaccgtca cgtccgacct cggcaccgtc aacaacgatc caaggctcgc gcgccgcatc
     1861 gagatcgact accagtcgcc ggcagtgacc ggcgaagcgc acgctggctt ctaccgcgat
     1921 gacagcggca ccgtgatcgc gatgggcacg atgaccacgc aggcctacgt gatgattccg
     1981 gcgtgcgcga cgttcaacaa caaggccggc atcctcatct atggtcacgg catcttcggc
     2041 ggcctgcccg agctgcggaa cggcgagtac gcgcgcgatc tcgcgcagga tggctgcttg
     2101 atcgtcgccg gcacggtgtg gaccggcatg tcgaatgacg acatcccgaa cgcgctgctc
     2161 gccctcaacg atctcaacaa agggaagggc ttcggcgagc gcatcttcca gggcatcgtc
     2221 aacacgatcg cgctcgagca gctgctgcgc ggcaagttcg cgaccgagct gttcatcgat
     2281 tcgcaaggcc gctcgatcgt cgacacgtcg cgcgtctact tcctcggcgt gtcgatgggc
     2341 cacgtgctcg gcaccgtgtt catggcctac gatccgttca tcacgcgcgg cacgctcaac
     2401 gttggcggcg cgaactgggc gctgatgttc gaacgctcga acagctgggc cgcgtatgga
     2461 ctgccgatca agagctcgta cgacacgctg ctcgatgccg tgatcatgga gcaagtgctg
     2521 cagatgtcgc tgcagccggc agatggcgcg acgatcgccg gcatcagcgt accgaacaca
     2581 ccggagaagc acttgctgct gcagacgtcg ctcaacgatg cgtcggtgcc gaacgtcgcg
     2641 agcttctacc atgcgcgttc ggcgggcgtc gatctgctgt cgacgacgat cgtcacgccg
     2701 tacggactcc ccgctgcggt cgactcgtcg acgaagagcg cgatcgtcgt cgtcgacgag
     2761 aagccatcgc aggtgccgcc cgagacgaac gaggtcttca acttcaacac gatcgcgcac
     2821 gagaacccgc ggcggcggac gctgctgcag caacagatgc gcgatttctg gtcgacaggc
     2881 acgatctcca acgtttgcac cggcccctgc gactgcgccg cgggtgcgtg cgggacgctg
     2941 cgaaccgcga tgtacggcgg cagttaattg cgcggttccc cgacgggaac gcagctcctg
     3001 cgcgcttttc gcactgccat cgggatttcc gccggattcg gctggcatca caaatgctgt
     3061 gagtcgggtc atgagcgcaa accacagcca ggtcgtcgtc gggttcgatt ttagccacag
     3121 cgggcgggca gctctcgatc gagcgatcgg gctagcgacg cgagcgccgt ggcacgtgct
     3181 ccatttcgtg tgcgtgatcg atccgcactt tgccttcccg gccttgccga cgaagcacat
     3241 cgatctcgag tacgcgaatc gcgtgcaaga ggaggtgacc gcgatcgtcg agcaagagct
     3301 aaaggcaaaa cgcacggaca tgccgatcca cttcttcgtg cacgcgcgcc tcggcaaacc
     3361 ggcggcagag atccttggct gtgcgcgcga tgtcggcgcc gacctgatca tcgtcgggag
     3421 caaaggactc acgggtatcg agcgcgcagt gctcgggtcg gtctcggaga aggtggttcg
     3481 tgaggccggc tgcagcgtcg aggtcgcgcg gcccaaaacg tacgagtacg tgccgctcct
     3541 cgcgatcacc gacgcccagc agcaccagaa gtacgcggcg ccgcatcgct acacgtacga
     3601 agaccgtcgg gcaagtcgcc gccccaacga ctggccgctc tattaagccg ggaataaacg
     3661 gctagctaga tcgacgtagg ccacgtgcta gccttcagac gtggcgaagc tgcgaatcac
     3721 gtacaacgca ccggtcgtcc tgaccttcgc tctcgcggcg gtcatcgcgt tcatcctcga
     3781 gaagacgatc agcggatcgg tcgagtggtt cgccgcgtat ccgcatctcg atcgcacgca
     3841 ggcctacgtc ggtctgttca cgcacatcct cggtcacgcg aactggcagc acctgatcgg
     3901 caacttcatg ctgatcctgc tgctcggtcc gatcctcgag gagcgacacg gctcgggccc
     3961 gctgctgctg atgatcttgc tcaccgcatt catcaccggc atggcgaacc tcgtgttctc
     4021 gaacggcgcg ctgatgggcg cgagcggcat cgtgttcatg atgatcctgc tcgcgtcgat
     4081 ggcgaacgtg cgaggcggcg agattccgct cacgttcatc gcggtcgccg tcatctacat
     4141 gggcggcgag atcattcgcg cgttccgcga ggatcagatc tcgcagatgg cccacctcgt
     4201 cggcggcatc gccggcgcgg cgttcggctt catcatcgcc ggcaagaagg gtgtggcgaa
     4261 ggcgccgaag ggcggcaccg ccgccgacct cgagaagatg ctcgctccgg cacgcgcgca
     4321 gaccgcaaag cccaaagcga agatctgacc cgaagtccga agcctgaggc ccgaagcctg
     4381 atcagacgaa gaagtcgggc atcagcttct gcgacggatc gactgcgtac tcgctgaggt
     4441 ccgtcttgcc cgtcgacatc atcacttcgt catcgatgaa gaaattgcca gtgcactcgc
     4501 gcgccggtcg gttgaagatc acatacgccg cgtcgcccat gatgtcgggc ttacgcgacg
     4561 ccttgatcat ctgatcgccg ccgagcaggt tcttgaccgc ggcggtcgcg atcgtcgtgc
     4621 gcggccatag cgcattgaac gcgatgccgg cgctcttgta ctcgcctgcc atgccgagca
     4681 cgcacatgct catgccgaac ttcgccatcg tgtacgcgac gtgcggcgcg aaccacttcg
     4741 tctccatgtt gagcggcggc gacagattca agacgtgcgg gttctgggcc ttcttgaggt
     4801 gcgggatgca gagcttgctg cataagaacg tgccgcgcgt gttgatgccg tgcatcaggt
     4861 cgtagcgctt catgtccatc tgctcggtgc ccgtcaggtt gatcgcgctc gcgttgttca
     4921 cgaggatgtc gatgccgccg aacgtctcga cggtcttcgc gacagccgcg gccacgtgat
     4981 catccattcg gatgtcgcat gcgatcggca gcgccttgcc acccgctgct tcgatctcct
     5041 tggccgccgt gaagatcgtg cccgggagct tgggattcgg ctcggcggtc ttcgcgacga
     5101 tcgcgatgtt ggcgccgtcg cgcgctgcac gcagtgcgat cgcgaggccg atgccgcggc
     5161 ttgcgccggt gatgaacaga gtctttccgc gcagtgacat gcgcggaacc tagcacgatc
     5221 aaccctgatt acagtctgcg ctgactgttt atgttcagtc gaccgtccac tcgggctctg
     5281 gtggccgatt cactttcgcc atcatctgcg ctcccgcgat ccccatcacg cacccgacac
     5341 cgaagatcca gaacgacagc ccgaccccga ccatgccggg cggtcctggc ttcgtcgcga
     5401 cgaacacgca gcccgtgatc agcccaatca tcacgccgag tagcgcgatg ctcgtcggtg
     5461 cgatcggcca gctcatc
//
LOCUS       DASUFX010000134         4335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_716131,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000134 DASUFX010000000
VERSION     DASUFX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716131"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFV99_05245"
     CDS             <1..410
                     /locus_tag="VFV99_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058743.1"
                     /translation="GIEEVGKRLQEARDTIVKLNGIVGKIPDREHPGDNRSALERQAE
                     AAAKAGKVADLQKLLHDSEEELERGLTIINDDLDAVEAWLANYDRKTLALNVAAPAGG
                     QTGEQAPAANPEQPVNPAQPPAPAPAQPEPPKQ"
     gene            432..1943
                     /locus_tag="VFV99_05250"
     CDS             432..1943
                     /locus_tag="VFV99_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX5058744.1"
                     /translation="MRIPANPKVILRSCREYDPEKIRTIIREGLEELGLRPFGRTLVK
                     PNLVAAGPLFPNAFTRPEFGEGVLRALRDVGGDQDNGSIKELAVGERCGITVPTRVPF
                     RESGWDDMLKKVGVKKYHFEEQQQVEIPLTHENRLRDYIFTPEPIARADFFVNCPKFK
                     AHPWTTVTFSLKNYIGIQDDRHRLIDHDHKLNEKIADLQHIIQPQFIAIDAITAGEGR
                     MLTPTPFDLGMIIMGNSQVAFDAVCCAIIGIDPMTVDHIRLAAEHGFGTTDLKKIEVT
                     GDISLDDAKKRAKGFKVGLIRVEKYFEGTHISAYAGPPPESEHGDYCWGGCPGAIEEA
                     IEILRQFDKHTDEKMPRLHVVFGAYDGQIPAKPGEKVIFIGDCANWQGELHGQPIDIK
                     NLYKDRSTMDPHSASSEDIFVKLSKAGAKMRGDVVRLEGCPVSVSEQVLALVAVGKLN
                     NPFLDPSQAATFTRGYLGWKSRVLLNKLQLKRYQKNGAFAERGQAAPDLGGTS"
     gene            2075..2359
                     /locus_tag="VFV99_05255"
     CDS             2075..2359
                     /locus_tag="VFV99_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058745.1"
                     /translation="MNTEIVLFDYGPAEFPVASMRPRETRARIVAALASLQSWFFARW
                     RWLRPRSVPCAVAMLGMLAVIAAANYLAHYHGACPHDAPAAAVYIEIAHG"
     gene            2378..3202
                     /locus_tag="VFV99_05260"
     CDS             2378..3202
                     /locus_tag="VFV99_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5058746.1"
                     /translation="MTIRAVHHLNCATMCPIAGFLLGGNSWRGKMVAHCLLLETERDG
                     LVLVDTGFGTRDVEGTSGLSKSFRALCGPALSHAETALAQVQALGYKPTDLKHIVVTH
                     LDLDHAGGLVDFPHAKVHLHAREHAAAMARKHFKERERYIAAHWAHGPKWEVYSEDGD
                     TWRGLPAVTRLRGIDADIGLLPMHGHTRGHSAVIANSGDRWLVHAGDAYFHRSSIEGS
                     SKVPFGFAAFERATEMNPAARRASLAALRQLAASYPDLDLFCAHDEREYSALRGRA"
     gene            3311..3796
                     /locus_tag="VFV99_05265"
     CDS             3311..3796
                     /locus_tag="VFV99_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5058747.1"
                     /translation="MVRLALAPLLLVGCVQVFGLDDPFPEGGDGSGSGVNEACTGAVV
                     AGTCYERFAGPAIWTDARDACAKNGARLAKMESPDVRSAVGGLLGLDDEAYLGATDEA
                     QFGTYLWLDLSPVTYAGWLPTQPDGDPDEHCIILHNAGAIGWEDDKCDDERAYICEHK
                     L"
     gene            complement(3824..>4335)
                     /locus_tag="VFV99_05270"
     CDS             complement(3824..>4335)
                     /locus_tag="VFV99_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058748.1"
                     /translation="ATAFHGIPQATFTVDADPTKTTMTAPNGRFILCVAPSDGLATIT
                     PMGLSANYIGGKVKIDKTVIQSGATLSYRSFTMARAADFGFDAGLAHVFVHVHGGTRT
                     VTTADAPAVKKHFDGTSWVDGNTGDNIYLGNIAGTTTAIQVSGGGATGASSIPLMAGA
                     FTYVTIIAK"
BASE COUNT          782 a         1427 c         1420 g          706 t
ORIGIN      
        1 ccggcatcga ggaggtcggc aagcgcctcc aagaagcccg cgacaccatc gtgaagctga
       61 acggcatcgt cggcaaaatc cccgacagag agcacccggg tgacaaccgg tcggcgctcg
      121 agaggcaagc ggaagccgcc gcgaaagctg gcaaggtcgc cgatctgcaa aagctgcttc
      181 acgacagcga ggaagagctc gaacgcggcc tgacgatcat caacgacgat ctcgatgccg
      241 tcgaagcgtg gctcgccaac tacgatcgca agacgctcgc gctaaacgtc gctgctcccg
      301 caggtggtca aacgggcgag caggctccgg cagcgaatcc cgagcaaccg gtgaaccccg
      361 cccagccgcc agcgccggcg ccggctcaac ctgaaccgcc gaagcagtag cctcgctgct
      421 atgctgggat catgaggatc ccggcgaacc ccaaagtcat cctgcggtcc tgccgcgagt
      481 acgacccgga gaagatccgg acgatcattc gtgaagggct cgaggagctc gggttgcgtc
      541 cgttcgggcg cacgctcgtc aagccgaacc tcgtcgcggc cggcccgctg tttccgaacg
      601 cgttcacgcg gccggagttt ggcgagggcg tactgcgcgc gctccgcgac gtcggcggcg
      661 accaggacaa cggctcgatc aaagagctcg ccgtcggcga gcgctgcggc atcacggtgc
      721 cgacccgcgt gccatttcgc gagtcgggct gggacgacat gctcaagaag gtcggcgtca
      781 agaagtacca cttcgaagag cagcagcagg tcgagattcc gctcacccac gagaaccggc
      841 tgcgcgacta catcttcacg cccgagccga tcgcgcgcgc cgacttcttc gtgaactgtc
      901 cgaagttcaa agcgcacccg tggacgacgg tgacgttctc gctcaagaac tacatcggca
      961 tccaggacga tcgacatcgc ctgatcgatc acgatcacaa gctcaacgag aagatcgcgg
     1021 acctccagca catcatccag ccgcagttca tcgcgatcga cgcgatcacc gccggcgaag
     1081 gccgcatgct gacgccgacg ccattcgacc tcggcatgat catcatgggc aacagtcagg
     1141 tcgcgttcga tgcggtctgc tgcgcgatca tcggcatcga tccgatgacg gtcgatcaca
     1201 tccggcttgc ggccgagcac ggcttcggca cgaccgatct gaagaagatc gaggtcaccg
     1261 gcgacatctc gctcgacgac gcgaagaagc gcgcgaaggg cttcaaggtc ggcctcatcc
     1321 gcgtcgagaa atacttcgag ggcacgcaca tctcggcgta tgcgggaccg ccgcccgagt
     1381 cggagcacgg cgactactgc tggggcggtt gcccgggcgc gatcgaggaa gcgatcgaga
     1441 tcctgcggca gttcgacaag cacaccgacg agaagatgcc gcgtctgcac gtcgtgttcg
     1501 gcgcgtacga cggccagatt ccggcgaagc cgggcgagaa ggtcatcttc atcggcgatt
     1561 gcgcgaactg gcagggcgag ctgcacggtc agccgatcga catcaagaac ctctacaaag
     1621 atcgcagcac gatggatccg cacagcgcga gctccgagga catcttcgtg aagctgtcga
     1681 aggcgggcgc gaagatgcgc ggcgatgtcg tgcggctcga aggctgcccg gtatcggtgt
     1741 ccgagcaggt gctcgcgctc gtcgcagtcg gcaagctcaa caacccgttc ctcgatccgt
     1801 cgcaggccgc gacgttcacg cgcggctacc tcggctggaa gtcgcgcgtg ctgctcaaca
     1861 agctgcagct caagcgctac cagaagaacg gcgcattcgc cgagcgcggc caagcagcgc
     1921 cggatctcgg aggcacctcg tgatgggacc gggctcgttg cctcggtacc gcccgcgcgg
     1981 tgtgttcccg ttgtcgaagc gcaccctcgc ctacacgcgc atgcggcgca aggcgcgatc
     2041 gtcggcgaat aaacaggcgc accacacggg acctgtgaat actgagatcg tgctcttcga
     2101 ctacggtccc gcggagtttc ctgtcgcgtc gatgcggccg cgtgaaacgc gcgcgcggat
     2161 cgtcgcggcc ctcgccagct tgcagagctg gttcttcgcg cgctggcggt ggctgcgtcc
     2221 gcgcagtgtg ccgtgcgccg tcgcgatgct cggcatgctc gccgtgatcg ccgcggcgaa
     2281 ttaccttgcg cactatcacg gcgcctgccc gcacgacgcg cccgctgctg ccgtgtacat
     2341 cgagatcgca cacggctaac gcggtatcgt cggcgcaatg acgattcgcg ccgtccatca
     2401 tttgaactgc gccacgatgt gcccgatcgc gggctttctg ctcggcggca attcatggcg
     2461 cggcaagatg gtcgcgcatt gcttgttgct cgagaccgag cgcgacggtc tcgtgctcgt
     2521 cgataccggc ttcggtacgc gcgacgtcga gggcacgagt gggttgtcga aatcatttcg
     2581 cgcgctgtgc ggacctgcgc tgtcacacgc cgagaccgca ctcgcgcagg tgcaggcgct
     2641 cggttacaag ccgacggacc taaagcacat cgtcgtcacg catctcgatc tcgatcacgc
     2701 gggcgggctc gtcgactttc cgcacgcgaa ggtccatctg cacgctcgcg agcacgcagc
     2761 cgcgatggcg cgcaagcatt tcaaggaacg cgagcgctac atcgccgcgc actgggcaca
     2821 cggtccgaag tgggaggtct atagcgagga cggcgacacg tggcgcggcc tgcctgccgt
     2881 cacgcgcctg cgcggcatcg atgccgacat cggcctgttg ccgatgcacg ggcacacgcg
     2941 cggtcactcg gccgtgatcg caaactctgg cgaccgctgg ctcgtgcatg cgggcgacgc
     3001 gtactttcat cgcagctcga tcgagggctc gagcaaagtg ccgttcggct tcgctgcatt
     3061 cgagcgagcg acggagatga atcctgcagc gcgccgcgcg tcgctagcgg ccctgcggca
     3121 gctcgcggcg agctaccccg atctcgatct gttctgcgcc cacgacgagc gcgagtactc
     3181 ggcgttacgg ggccgggcgt agcaaacaac catccccacc gcgcgcggga gtttcccacc
     3241 caggtcacgc gaactcgcgc gaaaccgcga gcggatggcg atggtgcgcc cgttgcagtg
     3301 cgcgacctgt atggtgcgtc ttgcgctagc accactgctg ctcgtcggtt gtgtccaggt
     3361 gttcgggctc gacgatccgt tccccgaagg tggcgacggg tctggctccg gggtgaacga
     3421 agcttgcaca ggtgcggtcg tcgccggcac ttgctacgag cggttcgccg gtccggcgat
     3481 ctggacggac gcgcgcgatg cgtgtgcgaa gaacggcgcg aggctcgcaa agatggaatc
     3541 gcctgacgtg cgatcggcgg tcggcggtct gctcgggctc gacgacgagg catatctcgg
     3601 cgcgaccgac gaggcccagt tcggcaccta tctgtggctc gacctgtcac ccgtcacgta
     3661 cgccggctgg ctaccaacgc agcccgacgg cgaccctgac gagcactgca tcatcctgca
     3721 caacgcaggc gcgatcggct gggaagacga caagtgcgac gacgagcgcg cgtacatctg
     3781 cgagcacaag ctctgagcgt gcttgggcgt ccgcgacgga ggcctacttc gcgatgatcg
     3841 tgacgtaggt aaacgcgccc gccatcagcg ggatcgagct cgcgccggtc gcaccgccgc
     3901 cgctgacttg aatcgcggtc gtcgtgcccg cgatgttccc gaggtagatg ttgtcgccgg
     3961 tgttgccgtc gacccagctc gtgccgtcga agtgcttctt taccgctggc gcgtcggccg
     4021 tcgtcaccgt gcgcgtgccg ccgtgaacat ggacgaacac gtgcgcgaga cccgcgtcga
     4081 agccgaagtc cgcggcgcgc gccatcgtga acgagcgata gctgagcgtc gcgccgctct
     4141 ggatcacggt cttatcgatc ttcaccttgc cgccgatgta gttcgcggag agtcccatcg
     4201 gcgtgatcgt cgcgaggcca tcgctcggcg cgacgcacaa aatgaagcgg ccgttcggcg
     4261 cggtcatcgt ggtcttcgtc ggatcggcgt cgaccgtgaa cgtcgcctgc ggaatgccgt
     4321 ggaacgcggt cgccg
//
LOCUS       DASUFX010000135        24735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1551650, whole genome shotgun sequence.
ACCESSION   DASUFX010000135 DASUFX010000000
VERSION     DASUFX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24735
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551650"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFV99_05275"
     CDS             <1..859
                     /locus_tag="VFV99_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5058749.1"
                     /translation="ETTKTKDITGGLPRVAELFEARKPKDHAVICDIDGVVHFGKDTK
                     GKRKVLIVPEVGDPAEYLIPKGKHLSVREGDRVRAGEALMDGPANPHDILKVLGERAL
                     AKYLVDEIQEVYRLQGVRINDKHIEVIVRQMLRRVSVSDPGDTNFLVDEHVEKHIFEE
                     ENERVIVAGGQPAKGDALLLGITKASLSTESFISASSFQETTKVLTEASINGRVDYLR
                     GLKENVIMGRLIPAGTGLGAYKRLTVDVETEDEDEDLAEGGSYSSHGGAMDMPRDSDD
                     MGSTAEALE"
     gene            complement(903..2201)
                     /locus_tag="VFV99_05280"
     CDS             complement(903..2201)
                     /locus_tag="VFV99_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058750.1"
                     /translation="MRVRWILLLAMAGVIACGYPGLMAADSADQLDLARRWEHGDWHP
                     ATMSVLWRAIEVVIAGPVGMFVLQVVGFIVGAYWVLRHRLQDKHAAIVVVALSWFPPV
                     LTVMLVVWKDAPMAAFLLLGIAGLLASRRRWNWLGLGALLFASAMRHNGAAATFAPLL
                     LLWSVGTGWRRIAMAAGLWVAITVASVGINNALATRQEHPWVVSLAVTDIVGTIRYAP
                     PIDDAEARQLLAGAPLTVDHDIQSTIVTRYKPRGWNAYYADNPQITWPATDAERDGVT
                     HAWRSVVRAYPLAYLQYRWEVFKRVIRLGIRPRVPTPMFDFAFTGDKDLYERLHHEAS
                     PSDIQRAAFWLLRRVGGIWYVPFIYIVISLVLLPLARGDRLVMALVGSGIGYELSYFF
                     FAPAPDYRYSQWLIVTTILAAVIVFARRYATAREQQKSPA"
     gene            complement(2678..3142)
                     /locus_tag="VFV99_05285"
     CDS             complement(2678..3142)
                     /locus_tag="VFV99_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058751.1"
                     /translation="MLTYLAALLIGVIAGLRTFTAPAAVSWAAYFGALPLAGTRLAFF
                     GAGLTPYIFTALLIWESIFDQLPRVPTRKSPPQVIGRVASGALCGAAVVMPHSWWVFG
                     LCEGIIGALIGTFAGYELRVRLARAVGRDRPVAIIEDVVAVVAAVLIVTLLV"
     gene            3348..5642
                     /gene="gspD"
                     /locus_tag="VFV99_05290"
     CDS             3348..5642
                     /gene="gspD"
                     /locus_tag="VFV99_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX5058752.1"
                     /translation="MRWLIALLAVSVPVAASAETVDESIYNCKPRDAAVEITFKPEIE
                     LKELLSWAVGFTCKKFVFDPRIVSTGRKVTLVAPGKQTPAQAYDMFTTALGTLGLTVV
                     PKGRLLEIVESAVGKTKTPPIYAPNQVPTASEQIVRYLMRPQYAQPETMRQAVTAFKS
                     DAGDVQVIGSLVLVTDHASNIRDMASIAKLIDVPGGADGIYAIPVKHADASKLAEKLG
                     QLLDVSAGASIGRGPTKGDQQVTRDTTQTPSKLLVDERTNTLIVSGTDAAFQRVKALV
                     ERLDISLEIEGGASMHVYQLGSAISEELAKTLNDAIQAQTQSGQGDKKAPPGTASGDL
                     TQLEGPVRVISDKPTNKLIVMSSGRDFLAIREVIRELDVPRRQVYIEAMILEVQMGNG
                     LSLGTSSHGGLNYGADNSSLLIGGVQAPSLKSTNLETLTGASGLIGGLLGKTLPGSEA
                     LFGKSIPSYAVLFQALADTSRMNILSTMPLIVVDNEQAKYKLGTNVPYIKGVMPTSVT
                     NTQLTTNIDRKDLLLELEIKPHISTDDSVLLEVRQASEDLGDSNSQLGPSWSTRSIET
                     RVLVRDQQTIVLGGLIQNREQIATSKVPLLGDIPILGHLFKYTSKSQKKMNLLVMLTP
                     YIVKDNLDLENIRARKQREYEEFAGSFRHLDGQKYLPRIDYTRKRGVVEEINRIVLDI
                     EAETAARGKLHTAVSVSPGLVEPARFEAEAPDDKPAPEEKPAQVAPPTPSSAAPRIVP
                     SGFSTTGTAATPRAPWQTPMGFAD"
     gene            5870..6571
                     /locus_tag="VFV99_05295"
     CDS             5870..6571
                     /locus_tag="VFV99_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5058753.1"
                     /translation="MAKQQVQGELDLKPRTWGGAREGAGRKPNANKRDPAHRVRPALK
                     RYHPVHVVMRVKKDVPRLRTGKAYRAIRRVLVKCLGNETFRVCHLSIQRNHLHFLVEA
                     NDKRALSSGMQRLNILAARALNAELGRSGSVFEFRYHATPITSPKQARNALAYVLNNW
                     RRHDEDESCERAQTAKLDPYASGLSFAGWDGIAFAKPVDFTPLSVSEPQTWLLRVGWR
                     RHGLLRVDEVPGRLH"
     gene            complement(6847..7323)
                     /locus_tag="VFV99_05300"
     CDS             complement(6847..7323)
                     /locus_tag="VFV99_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEX5058754.1"
                     /translation="MSGKRGGNEPAQAVRFYSVNAEYGEFSNFAPYPITLRGKRWPTS
                     EHFFQAQKFAAIKDQDEIRAVASPMIAARMGRDRKRKLRRDWERVKVGIMREAVEAKF
                     RQHGELRTLLLSTGDAKLVEHTDRDDFWGDGGDGSGRNELGRILMAVRESLREDVE"
     gene            7313..7603
                     /locus_tag="VFV99_05305"
     CDS             7313..7603
                     /locus_tag="VFV99_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058755.1"
                     /translation="MPDIDARMTRLGTIANWCYPDAMKIELNADEALVLFEWIMRFSD
                     RGTYPVEHEAEELVLWQIEAQLETTLVEPFEPNYVQLVEQARARIVADQRAR"
     gene            7655..8032
                     /locus_tag="VFV99_05310"
     CDS             7655..8032
                     /locus_tag="VFV99_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058756.1"
                     /translation="MELTPLEAAVLDMLLAGDDPWLAILREQRRLLHVTGREHSGVGF
                     SADLAVPADAPKLPGGATVRFGDVSADIDGLEQGAGFLLFIDGGVITVLEGYLYGDAE
                     WPVEVTKFTLRYDREPRDLAPLH"
     gene            8142..10340
                     /locus_tag="VFV99_05315"
     CDS             8142..10340
                     /locus_tag="VFV99_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5058757.1"
                     /translation="MRVAALALVAVMGCVHSESVMCGDRACPGGTECDDVHMLCIDHD
                     QRTACNTSANGDACTTSKRAGICDQNVCLPGCTDGVADPGEECDDGNRVDHDGCSATC
                     VTEEPTWTVWQSPWTPRSGHMAAYDSDRKRVVVFGGADANGATDDLWERDTDGAWTQI
                     DITRPSSRAYGAMAYDPVRKVTVLFGGASKLGTLLRDTWEYDGTSWKQITPPTSPTGR
                     AYSAMVFDPIRQRITLVDGLDGVDVPASGLVADVWQYDGTTWTQLTTTGTPPPRRAHA
                     LAWDTARQRLVMFGGIRPIPPTTAATSETWELSFGTSWTWTCTSGEATSPSACPQAVT
                     RPTNRYSSSLVYSPLHNTIVMYGGLTNAGTATQNASDTWTYGATGWTATTQAVSPGGR
                     SAAALSDCEIQIDATTFQHRPVLIGGSTDEGATDEVWELTSAWLRTLTPGNAPPRYDI
                     ALSYDAKRDRTVSVGGYLPPLARADTFVFDGKVWKRQPPLPAIRFAMSATYDSTRELV
                     GVFGGLTQSSNRTNETYALTGLTWMQLPGPAPSPRYGAALAHDPRASVNVLFGGNDMS
                     GTNLRDTWELDMNGWSQNMSAGGPPAQLGAAMAFDPERQELVLFDGAAKTWLYTNRVW
                     TPLETTASPPARNGARLVYFPSRKRLVLYGGVADNGTSTTLLTDMWELDTDGDARAWH
                     EVVYANPPPPRRGFGFVAHASLDSLVLRAGQTSSGEPLDDTWLFRWQSAK"
     gene            10411..11544
                     /locus_tag="VFV99_05320"
     CDS             10411..11544
                     /locus_tag="VFV99_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058758.1"
                     /translation="MRGMLIAFVSLTACASGGDKPDAAPARDTAVDVPTVVSAVCPSG
                     QLAVDVSSSKELTCVPLDGFTRSALADHCSVYLGWRDSCDACTTPPAKWGRVETGSCS
                     PGTGTNNTCTMPTLGGTPVHMFGLDLDGDMDGNDKLYAGLHCTSEDDSGAIAPCPAGQ
                     LVNGFNGTAWSCASYAAAVVSYVHANCSIYLGWQDSCDGCTSAPPKWGLTSNDTCMNG
                     AGTDNTCIAPTQLGLEQVRLFGLNTDGDVDGNDKLHVGLRCEPPGQGSSIQTSMCPTG
                     QFVAETLTQGGFRCESPAATVAQYFGSHCTLYFGWHDSCDGCITPPTKWGTVRVGACM
                     NGTGVDDTCSKFTLGGQSVDMFGLSPDGNVDENDVLYVGFRCE"
     gene            complement(11632..12099)
                     /locus_tag="VFV99_05325"
     CDS             complement(11632..12099)
                     /locus_tag="VFV99_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058759.1"
                     /translation="MRLLALFVLTIAASCGGSPKPAAPPPTPDPIPKTAGPSCDVVAD
                     KLAIVIHADQPDAQADAKTQLRSRCTGDKWSDEARSCFATVESDAELDGCRQHLDDAQ
                     KSGFAKVTGGDGAAAPAAAAAAPAAEPPKPKAKRTTRGAQPKGDSSDPQEGGE"
     gene            12138..15212
                     /locus_tag="VFV99_05330"
     CDS             12138..15212
                     /locus_tag="VFV99_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5058760.1"
                     /translation="MRLLGTALVVAVWLCSVAEAKPKQTAAPKPTPAATAPGKPAKPG
                     KQATPQQQAEIAKLEQELTQHQIKQAYFAALKVAKQLWDLQKKATGEDSPEAQRRKAA
                     VASAMSNAGDYAGAQKLYEEILHTTEKEKGPESREALYALMPLTSTYWSQSRFGEVEP
                     LMKRQLELTKKVEGEKSPMYSGQLQMYAGFLTLRNEFSAAQRVYEQVLQLAESTATSK
                     TDISLLGPVQALANSFWQTNQQQKAIQLFERAITIVDAQTVNGPIMKMSTRWGIAAQY
                     SYGGRKDLGLPLMKKAIEIGEKDIAQLEKDKPDDYQIPAMLGQIGYMYRQIGDLPHAE
                     KAYLKTIALAEKKKTYSGYEASLADLRREQGKPKEALALLEKGRDQMAKLSPISATVY
                     NTQIAQVLSELGEYKRAMQLLDEHRKFVIKQYGKKHPMYITAQMSAVNVYAAAGEIKE
                     AESILDDALDAAERDLANVMRSGTDADHAIYFARNSYILDTALNFGFNYAPNSGSAAR
                     LALTTLLRRKGRILDAAAASLATIRSKLSPDDKKILDELNSARAQLAKLVNAGPGATG
                     GDADAYGKEVAALEEKVLKLEIAVSKKSAAYRTVTQPIELAAIQKVIPSDARLIEIVN
                     YQPWDPRMAYTISPKLQPRHYAAYVLGAKGDPAVIELGEAAAIDKAIEDFRKAVADPD
                     NDRAGDLGNALFKLTMGKIVPKLGGATNILVAPDGALNVVPFSALVDDNKQFLIKKYT
                     FTYLTSGRDLLRVGLKTKAQGGGVVFADPKFDSTGPAAPGGGTTRGARSVDLRSLRWV
                     PLPGTGQEADALIKAMKNLKVYRGDQATEGALKGLHAPKILHLATHGFFLPDEAPPPA
                     PDQSQGGPPMGLAMPGSAVNTSVGSTYENPLLRSGLALAGANKLQSGDDDGVLTALEA
                     SGLDLWGTKLVVLSACETGVGKVSNGDGVYGLRRSLVIAGAESLVMTLWQVDDFATKD
                     LMAGFYKKLAAGKPRSAALREMQLEIAGQEKYAHPFYWASFLPAGALTPIKD"
     gene            15216..17714
                     /locus_tag="VFV99_05335"
     CDS             15216..17714
                     /locus_tag="VFV99_05335"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5058761.1"
                     /translation="MSLRPLLILCLLTATVAAGPKPKKAKQPPVKSPAAKLDKKQLEI
                     ERHEWMAQYYILRANDMKGAAKEYQAILKLDAKNLNASLALSSIYRRDKQDKQALDVL
                     VKATKANPASIDAWLSLGELQSQMNDSKGMKASVDKAIALDGWNTSAYWLLFDDASRR
                     LKGGEAAAKTEALAAARKIMETTPPRRRTGTLYKVAERAVVELSGEPIELTIYDAKAA
                     YAAAFETGLMGTINQQMDKARRGFDECTKSQPKNEECHYFLGLVYSSVKSSDSYDPKK
                     ALAQFAAAPTTPLAWVETARIQRSTDKNGDARTSLEKALSLDGDLAVAHIELGILDKL
                     DGKLDNAVNHFIAAMDADPYGATGSRALTELTKAKPTHPRVTEGMLSGKNIDVFSTER
                     YASIVHEIEKELGGVDPKAPEKAMLEDVVRRLADGSGIKMQFKVNLLNTDMVNAFALA
                     SGDVYVTRGMLDLIAKKTGKKPDANNDALGHILAHELQHVIRKHTINTAVFQEAVKDG
                     GRYLDASILTHVERIHEIDADREGMVMAFLAGYHPRGGIELMETMGKEMEIPKNLDHP
                     TFQERVEYLTEYWTNDVRYAFVSFKLGVGAMDRGAKLEATDMKKAVEAYEEAADDFKR
                     FRAMLPTVKEAANDLGIAYTKLGVLAMNKDESPLGRWQSRFSLERESSVKYVNLARDE
                     EKTSTRGTEKNRMPWQLREAIAAFKDGLAIDEGYNKARLNLAAAYIAGNQIDNANAMI
                     AKVSASGGVTDGDIELIRGIALAEGKDYDKAKASFEKALGSAAAKRAATYNIAKTLDL
                     AGKKDEAKKAWGQYLKLYPGGPWAAAATAASAKL"
     gene            17791..18201
                     /locus_tag="VFV99_05340"
     CDS             17791..18201
                     /locus_tag="VFV99_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058762.1"
                     /translation="MLGEKFTQAQGKVTGTRVIPGIDGGPPLVETSFHVQGKVYGVDV
                     TDIGTYTSRMVPGGTLVGEGQGVSMTPDGDSIVWKGFGVGKPTGRGLAAQYRYSMSFQ
                     TTSQKLSKLNGLLAVGEWEVDENGNCKGQGWEWK"
     gene            18241..19785
                     /locus_tag="VFV99_05345"
     CDS             18241..19785
                     /locus_tag="VFV99_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058763.1"
                     /translation="MTTVSTTPGPAAIGSDDKELISGQEVGEYRIEGVLGRGGFGTVY
                     GAVQPVIGKRVAIKVLSRKYAADQDIVSRFAAEARAVNQIRHRNIIDIFSFGQLPDGR
                     HYYVMEYLDGEPLDSHLRTYGAMALEEAIPILRAIARALDAAHAKGIAHRDLKPENIF
                     LANDPDGGVYPKLLDFGIAKLLAPEETTMHRTGTGVPIGTPYYMSPEQCRGRDVDHRT
                     DIYSFGIVVYRMLTGALPFEAPDYVELLYKQVSEEPALPSRVNPKLPESVDIAIAWMM
                     QKDREKRPKTVLEGVNALDPANPMRPTPLPRSHRPSVGPTGATGRPQQTPSEIAFAET
                     GVSSPNANAATGAQTSAPPNPRGTRITMVIVAGLVVAGIGVGVYMKTRPPHVTVVERD
                     KGPTGSAVPAQEPPHEPVKQPIAEPADAAATPETVMLELTGAPEGTEVLRDGKLIGVV
                     PKVPLPYSTKVQYLVLQADGFQPLPITVLPDQDRRMAVKLKPKKRAGGSRPSHDDVED
                     PFKKAD"
     gene            19785..22418
                     /locus_tag="VFV99_05350"
     CDS             19785..22418
                     /locus_tag="VFV99_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058764.1"
                     /translation="MRAFAIAVIAVIAAGCADLPAFEETCGNGVIDPDEDCDNNGPTC
                     DSQCRVTCANTVDCDALPGAYVCGADSICHAPSGVFVQTPAVAFPFVVGQGAVADLDG
                     DRIGDVVGFSDVTIDVRFGDLDADLGTRSTMLLPYSNTSIAIRRFDDATGHSDIVVPT
                     PDGLAAYTAASKQLVSYPFSSAPGASGDCVTLGQTADPVATFELDKHRLAFVARVFAT
                     GEARVGVLDVVTGECKSGPLCNLTLPDAQQLAGRLFTDHYETQASPLTSTIAVGFASS
                     MTSEQVCALRFTGTPGNFTFTALTATPLVAEPGPIVLARAQPAAGCPTLFTGGGGGIE
                     LYAAAGIPAACSLATLPSALPGFGANVLPTGRIPLVPAIAGAAPDALVVAKGVGFAAS
                     GDIYTLAVPTTTLMYHSPRPLFPVTAADLDGDGSIEAVASVLGAGGPQGQPDLDVLYR
                     TAMPVDSFVPYRITTQGVPTNLKLGDFDGNALADIAYTERIGTVERLMVAYGTRDRPL
                     PAVQITAFDQIVGFAKLDLPDSIEPTGQVLDDLVVINLPPTAMPTMTILHGNPQRSML
                     AYFDPRAMFTATDSSFVAALTGNFDATPGVDVLAFERYTPTAGSEAVRMWLAQGTVGS
                     QRLTGAGQPITTSLVTDCTQAGPLCLTGARFLTWPLGDRDVVIAADAQMRPGRSIVTI
                     DPMGPVTPATDPSTALPSEPFVFAMWGAELMATGQHKLLLSLGGIRGLGGTAKQVLDC
                     TVDMRGTVSSCVEFGGEVPELAGWTCQYAEPGRVAPRGRHDPPPRSAAGSVLALCKKE
                     GDGGRVVRISFGDGSYHAEPVLDHAENYEFLSIGDINGDSLDDVAGIAFDESFTPFVQ
                     IHLQCDARDVQCRNTNAGAPL"
     gene            22415..23629
                     /locus_tag="VFV99_05355"
     CDS             22415..23629
                     /locus_tag="VFV99_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5058765.1"
                     /translation="MMRIALALVMLCAATAHADRKNAEQLFRRGEKAYRAQRFLAAAQ
                     AFEAAYKELAAPELAFSAAQAYRRQYRVDPDHPEYVELAVKYYEIYLREVKSGGRTGD
                     AADSLDDMKAELRHLEKTGKLGKVTAETVPMLAVSVDFADRQQAPTKLHEIDDTRLTQ
                     AETPVNAFVDGKTVDPDVLLPVKEGEHVVRAEADGYKPAEKRVRVIAGVPAYEQLTLE
                     PLPAQLDVKTEAGATVIVDGRGVGTTPLAAISLAGGTHVVTVVRTGRKPAAREIHVER
                     AQTTSVSIELEPTVRRRAVPWVLAGAGVLTLGAGLSAVGAAYWNSKAVDRRDALIAGD
                     QDPSVLADYNDDRENRDRAITGVWVAGGAAVAVGLTAAWLYYFDSPSAEGVRVVPVAS
                     GSAGGAVISGHF"
     gene            23673..24071
                     /locus_tag="VFV99_05360"
     CDS             23673..24071
                     /locus_tag="VFV99_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058766.1"
                     /translation="MGSTGKRNLAAVATTLTFEKFWQWLGGHANCIVRAGTPEVVLID
                     HDDFHWTLISEDDHTKVVQLARAKDLVGEILVFSAEVAYVQCEPSESEGEWLFECIVE
                     SEKAREVAYHFVMAHEYDDGEHRREEKWTH"
     gene            24183..>24735
                     /locus_tag="VFV99_05365"
     CDS             24183..>24735
                     /locus_tag="VFV99_05365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058767.1"
                     /translation="MGAVFKAERVPVGKLVAVKFLHASYANDSEFQARFDRETRVMSR
                     LNHPNCVSVVDFGVWEAEPYLVMDFVDGVTLRSIMDKGPLPPNHALQIARQILSGLAH
                     AHKQEIFHRDVKPANVMISEEIGHGQRVRILDFGLARLQGNVGRDATQTNMVVGTPNY
                     MAPEQTVPGSTVDARTDLYAVGVI"
BASE COUNT         4600 a         8135 c         8092 g         3908 t
ORIGIN      
        1 tgaaacgacg aagacgaagg acatcaccgg cggtctgccc cgcgtggccg agctgttcga
       61 agcgcgcaag ccgaaagacc acgcagtcat ctgcgacatc gacggcgtcg tgcacttcgg
      121 caaggacacg aagggtaagc gcaaggttct catcgtcccc gaggtcggcg atccggccga
      181 gtacctgatc ccgaagggca agcacctctc cgtccgcgaa ggcgaccgcg ttcgcgccgg
      241 cgaggccctg atggacggcc ccgcgaaccc gcacgacatc ctcaaggtcc tcggcgagcg
      301 cgcgctcgcg aaatacctgg tcgatgaaat ccaggaggtc taccgactcc agggcgtgcg
      361 catcaacgac aagcacatcg aggtgatcgt tcgccagatg ctccgtcgcg tctcggtctc
      421 ggacccgggc gatacgaact tcctggtcga cgagcacgtc gagaagcaca tcttcgagga
      481 ggagaacgag cgcgtgatcg tcgccggtgg tcagccggcg aagggcgacg cactcttgct
      541 cggcatcacg aaggcgtcgc tgtcgaccga gtcgttcatc tcggcgtcgt cgttccagga
      601 gaccacgaag gtgctcaccg aggcgtccat caacggacgc gtcgactacc tccgcggcct
      661 caaggagaac gtcatcatgg gtcgcctcat ccccgccggc accggcctgg gcgcgtacaa
      721 gcgcctcacg gtcgacgtgg agaccgagga cgaggacgag gatctcgccg agggtggctc
      781 gtactcgagc cacggcggcg cgatggacat gccccgtgac agcgacgaca tgggctcgac
      841 ggccgaagcg ctcgagtaga gtcagacgtc tcgcgattgc gagatcgaga aagcccagcg
      901 tctcacgctg ggcttttttg ttgctctcgc gcggttgcgt accgacgcgc gaacacgatc
      961 accgcggcga gtatcgtcgt cacgatcagc cactgcgagt accggtagtc gggcgcgggc
     1021 gcgaagaaga agtacgagag ctcgtagccg atgccgcttc cgacaagtgc catgaccagg
     1081 cgatcgccgc gcgcgagcgg taggaggacc agcgagatga cgatgtagat gaacggcacg
     1141 taccagatgc cgcccacacg gcgtagcagc cagaacgcgg cgcgctggat gtccgacggc
     1201 gatgcctcgt ggtgcaggcg ctcgtacaga tccttgtcgc cggtgaacgc aaagtcgaac
     1261 atcggcgtcg gcacgcgcgg ccggatgccg agccggatca cgcgtttgaa cacttcccag
     1321 cggtactgca gatatgcgag cggataggcg cgcacgacgg agcgccatgc gtgggtcacc
     1381 ccgtcgcgct cggcatccgt cgcgggccag gtgatctgtg ggttgtcagc atagtaggcg
     1441 ttccagccgc gcggcttgta gcgcgtgacg atcgtgctct ggatatcatg gtcgacggtc
     1501 agcggcgctc ctgcgagcaa ctggcgcgct tcggcgtcat cgatcggcgg cgcgtagcgg
     1561 atcgtgccga cgatatcggt gaccgcaagc gagacgaccc acggatgttc ctggcgcgtc
     1621 gcgagcgcgt tattgatgcc gacactcgcg accgtgatcg cgacccacag cccggcggcc
     1681 atcgcgatcc gtcgccagcc cgtgccgacg ctccacaaca gtagcagcgg tgcaaacgtc
     1741 gcggccgcgc cgttatggcg catcgccgat gcgaagagca gcgcaccgag gccgagccag
     1801 ttccagcgtc gtctgctcgc gagcagcccg gcgatcccga gcagcaggaa cgccgccatc
     1861 ggagcgtcct tccacaccac gagcatcacg gtcagcaccg ggggaaacca cgacagcgcg
     1921 acgacgacga tcgctgcgtg cttgtcctgc agccggtgac gcaacaccca gtacgcgccg
     1981 acaatgaagc cgacgacctg gagcacgaac atgcccaccg ggccggcaat cacgacttcg
     2041 atagcccgcc acaacacgga catcgttgcc gggtgccagt cgccgtgctc ccagcggcgc
     2101 gccagatcga gttgatcggc ggaatcagcg gccatgagcc cgggatagcc acacgcgatg
     2161 accccagcca tcgcgagcaa gaggatccag cggacccgca cgtgctctag gtaccacgag
     2221 ggatgcgagc cgtggcactt cagctcgggc agcacgcgac agtaacgaag aatgcggcgc
     2281 cgagttgctg ctcctcgagg gtggcggtcg tgcctgaatc gagacacccg tagtgctggt
     2341 gcaggcagct ggcttgttgg cagcccgctt gcgcgcattg ggtcaagcgc acgcacggat
     2401 cggtcacgcc cgacgtgcgc cagccggccc aggttggagc tggcaccgtc gccaggttgc
     2461 tgcagttcgc ggcctgggcg gctcgtggtg ccggcgccgg ggtcgtcggc tgctgtgcgg
     2521 tcgtcggcgt cgagtcgctg gctgctccac acgcgcaaac caaaaccaaa gcaaggtagt
     2581 gcatgaccgc gttgtactgc tcgcgacgcg gcgtcgatgt caggtccgcg taagcgcgga
     2641 tgtcaggtcc gcgtaagcgt tgcgcgcgcg acgcgcatca cacgagcagc gtgacgatca
     2701 atacggcggc aacgaccgcg accacatctt cgatgatcgc gaccggtcga tcgcgaccga
     2761 cggcgcgagc caaccggacg cgcagctcgt agccggcaaa cgttccgatc agcgcgccga
     2821 tgatgccctc gcaaagcccg aacacccacc acgagtgggg catcacgacg gctgcaccgc
     2881 acaatgcgcc actcgcgacg cggccgatga cctgcggagg cgactttcga gtcggcactc
     2941 gtggcagctg atcgaagatg gattcccaga tcaggagcgc cgtgaagatg tacggcgtaa
     3001 ggccagcgcc gaagaacgca agtcgcgtcc ccgcgagtgg cagcgcaccg aaataggccg
     3061 cccagctcac ggcagcgggt gccgtgaacg tgcgtagccc cgcgatcaca ccgatcagca
     3121 acgcggccag gtaggtaagc atcaggtcct caccgatcgt aaggcgagca accatcggac
     3181 cagccgccgc ccaccggcgt acgtcgagcc acgcatggga ttgccgcgac ggcggaacgc
     3241 tcgcagtgca agcctgacac gttggcaacg ctgcgccgac cacgcaccgg gaatcgcgcg
     3301 cttatgtagt cgcgtgcgtt ggcccgcgag ttggtaatgc gaacgccatg cgctggctca
     3361 tcgcattgct cgccgttagc gtcccggttg cggcatccgc cgagaccgtc gacgagtcga
     3421 tctacaactg caagccgcgc gacgcagcgg tcgagatcac gttcaagccc gagatcgagc
     3481 tcaaggagct cttgtcgtgg gcagtcggct tcacgtgcaa gaagtttgta ttcgatccgc
     3541 gcatcgtgtc gacgggccgc aaggtcacgc tcgtcgcgcc gggcaagcaa acgccggcgc
     3601 aggcctacga catgttcacg accgcgctcg gcacactcgg cctcacggtc gttcctaagg
     3661 gccgtctgct cgagatcgtc gagtcggcag tcggcaagac caagacgcca ccgatctacg
     3721 cgcccaacca ggtccccacg gcgagcgagc agatcgttcg ctacttgatg cgtccgcaat
     3781 acgcgcagcc cgagacgatg cggcaggccg tcaccgcatt caaatccgac gccggcgacg
     3841 tccaggtcat cggctcgctg gtgctcgtca ccgatcacgc atcgaacatc cgcgacatgg
     3901 cgtcgatcgc caagctcatc gacgtcccgg gcggtgccga cggcatctac gcgattccgg
     3961 tcaagcacgc cgacgcgagc aagctcgccg agaagctcgg ccagctgctc gatgtcagcg
     4021 ccggcgcgtc catcggccgc ggcccaacaa aaggcgatca gcaagtcacg cgcgacacga
     4081 cgcaaacgcc atcgaagctg ctcgtcgatg aacgcacgaa cacgctcatc gtgtcgggta
     4141 ccgacgcggc gttccaacgg gtcaaggcgc tcgtcgagcg gctcgacatc tcgctcgaga
     4201 tcgaaggcgg tgcgtcgatg cacgtctatc agctcggcag cgcgatctcc gaagagctcg
     4261 cgaagacgct caacgacgcg atccaagccc agacgcagag cgggcagggc gacaagaagg
     4321 caccacccgg aaccgcgagc ggcgacctca cgcagctcga aggcccggtg cgcgtgatca
     4381 gcgacaaacc gaccaacaag ctgatcgtga tgtcgtcggg ccgcgacttc ctcgcgatcc
     4441 gcgaagtgat ccgcgagctc gatgtgccgc gccgccaggt ctacatcgag gcgatgatcc
     4501 tcgaggtcca gatgggcaac ggcctctcgc tcggcaccag ctcgcacggc ggcttgaact
     4561 acggcgccga caattcgtca ctcctgatcg gcggcgtcca ggcgccgagc ctcaaatcga
     4621 cgaacctcga gacgctcacc ggcgcgagcg gcctcattgg cggcttgctc ggcaagacgc
     4681 tgcccggctc cgaagcattg ttcggcaaat cgattccgtc ctacgcggtt ctgttccagg
     4741 cgctcgcaga cacgtcgcgg atgaacatcc tgtccacgat gccgctgatc gtcgtcgaca
     4801 acgagcaagc caagtacaag ctcggcacca acgtcccgta catcaaaggc gtgatgccga
     4861 cgtcggtcac gaatacccag ctcacgacga acatcgatcg caaagacctc ctcctcgagc
     4921 tcgagatcaa accgcacatc tcgactgatg actccgttct cctcgaggtt cggcaggcct
     4981 ctgaagacct cggcgacagc aattcgcagc tcggcccatc atggtcgacg cgcagcatcg
     5041 agacgcgcgt gctcgttcgc gatcaacaga ccatcgtgct cggcggcctg atccagaacc
     5101 gcgagcagat cgcgacgagc aaggtaccgc tgctcggcga cattccgatc ctcggtcacc
     5161 tgttcaagta cacgtcgaag tcgcagaaga agatgaacct gctcgtcatg ctgacgccgt
     5221 acatcgtcaa ggacaacctc gacctcgaga acattcgcgc ccgcaagcag cgcgagtacg
     5281 aggagttcgc cggctcgttc cgtcacctcg acggccaaaa gtacttgccg cgcatcgact
     5341 acacgcgcaa gcgcggtgtc gtcgaggaaa tcaatcgcat cgtgctcgac atcgaagccg
     5401 agaccgccgc ccgcgggaag ctgcacacgg ccgtctcggt ctcgcccggc ctcgtcgagc
     5461 ccgctcgttt cgaagcggag gcgcccgacg acaagcccgc acccgaggag aagccggccc
     5521 aagtcgcgcc accaacgccg tcatcggctg caccgcggat cgtgccgagc gggttttcga
     5581 ccaccggcac cgccgccacg ccgcgtgcgc catggcaaac gccgatgggc ttcgcggact
     5641 agcaccgcgc cgccgccctg ccgcgcgcct ggtcccgtcg cagtgacccg gccgcgcctg
     5701 gtccatttgg cgatttgtcc gcgacggtgg tcgcgtatcc gatgctcttg gatcgatgcc
     5761 tccgaggcgt cgtgattgcg cttctgcacg gtgtcatgga cctcgtttgt gtgctggaac
     5821 gacgccgcat cgtcgatgtc agaccgatct attatctgcc taaatcaaga tggcgaagca
     5881 gcaggtgcag ggcgagctcg atctgaaacc gcgcacgtgg ggtggcgcgc gcgaaggcgc
     5941 cggtcgcaag ccgaacgcga ataagcgcga cccggcacat cgcgtacgac ccgctctcaa
     6001 gcgttatcac ccggtgcacg tcgtgatgcg tgtgaagaag gacgtgccac gcttgcggac
     6061 gggcaaagca tatcgcgcga tccgccgcgt gctcgtgaag tgtctcggca acgaaacgtt
     6121 tcgcgtgtgc cacctatcga tccagcgcaa tcacctccac tttctcgtcg aggcgaacga
     6181 caagcgtgcg ctgtcgtcgg gcatgcagcg cctcaatatt ctcgcggctc gcgcgctcaa
     6241 cgctgagctc gggcgttccg gcagcgtctt cgagtttcgc taccatgcaa cgccgatcac
     6301 gagccccaaa caagcgcgga atgcgctcgc ctacgtgctc aacaactggc gccggcacga
     6361 cgaagacgag agctgcgagc gcgcccagac cgcgaagctc gatccctacg cgagcggcct
     6421 ttcgttcgcc ggttgggacg ggattgcctt cgccaagcct gtcgacttca ccccgttgtc
     6481 cgtatcggag ccgcagacct ggttgttacg cgtcggctgg cgaaggcacg gcttgctccg
     6541 cgtcgatgaa gtacctggcc gtctgcacta atttcgtcgc gtccgtgtag tgctccgcat
     6601 cggctaccgt acgaaacggt taggttcatc ggccccgtca cgtccgcgga atcgatcgcg
     6661 acctgcagcg ttcgccccga caccgcccac gcgccacgcg ctctcaggtc gtcgcgaccg
     6721 gtcatgggtc gtcgcgaccg gtcatggtcg tcaccgccgg tcgtggtcga ggatgattcg
     6781 atctcctcac gcaaatctcg gggaccacta tcaagctgcg tccgtgcagt gctctgcatc
     6841 aggtattcat tcgacgtctt cgcgcaacga ctcgcggacc gccatcaaga tgcgcccgag
     6901 ctcgttgcga ccactgccat cgccgccgtc accccagaag tcatcacggt ccgtgtgctc
     6961 gacgagcttt gcgtcgcccg tcgacagcag cagcgtccgg agctcgccgt gctggcggaa
     7021 cttcgcttcg acggcctcgc gcatgatccc taccttcacg cgttcccagt cgcgtcgcag
     7081 cttgcgcttg cgatcgcgcc ccatccgcgc cgcgatcatc ggcgacgcaa cggcgcggat
     7141 ctcgtcctgg tctttgatcg ctgcgaactt ctgtgcctgg aagaagtgct ccgacgtcgg
     7201 ccagcgcttg ccacgcaacg tgatcggata cggcgcgaag ttcgagaatt cgccgtattc
     7261 agcattcacg ctgtagaacc ggacagcctg cgccggttcg ttgccaccac gcttgcccga
     7321 catcgacgcc aggatgaccc gactcggcac aatcgccaac tggtgctacc cagatgcgat
     7381 gaagatcgag ctcaatgccg acgaggcgct cgttttgttc gaatggatca tgaggttctc
     7441 ggaccgagga acctacccgg tcgaacacga agcggaggag ctcgtgctgt ggcagatcga
     7501 ggcccagctc gaaacgacac tcgtcgagcc gttcgagccg aactatgtac agctcgtcga
     7561 gcaagcgcgt gcacgaatcg tggcggacca gagggcacgc tagagcgcag cttcgggacc
     7621 tggctgttcg tgctgcgcag gctacgctgt tgccgtggaa ctgacaccgc tggaggcggc
     7681 ggtcctcgac atgttgctcg ccggcgacga tccgtggctc gcaattcttc gcgaacagcg
     7741 acggctgctt cacgtcaccg gtcgagagca cagtggggtc gggttctccg ctgatctcgc
     7801 ggtgccggca gacgcgccga agctccccgg tggtgcaacc gtccgcttcg gcgacgtgag
     7861 cgcggacatc gatggtctcg agcagggtgc tggcttcctg ctgttcatcg acggcggcgt
     7921 gatcacagtg ctcgagggct acctctatgg cgacgcggag tggcccgtcg aggtgacgaa
     7981 gttcacgttg cgttacgacc gcgagccacg ggatctagcg ccgctacatt aggcgcgggc
     8041 ttcgtcgaac gggtgacaac gctgcgccgc agaccacccg tgtatcgtct ccgatcagct
     8101 gcgctagcga acgagcgccg caatcgtcta ccctggtaga gatgcgggtt gcggctttgg
     8161 cgctggtcgc cgtgatgggg tgcgtgcact cggagtcggt gatgtgcggc gatcgcgcct
     8221 gtcccggcgg caccgagtgc gacgacgtcc acatgttgtg catcgatcac gatcagcgca
     8281 ccgcgtgtaa tacctcggcc aatggcgatg catgcacgac gtcgaagcgc gctggcatct
     8341 gcgaccagaa cgtgtgcctt cccggttgca ccgacggcgt cgcggatccc ggcgaggaat
     8401 gcgacgatgg caatcgcgtc gatcacgacg gctgctccgc gacgtgcgtg accgaggagc
     8461 cgacgtggac ggtctggcag agcccgtgga cgccgcgctc cggccacatg gccgcgtacg
     8521 actccgatcg caagcgtgtc gtcgtgttcg gcggtgccga cgcgaacggg gcgaccgacg
     8581 atctgtggga acgtgacact gatggcgcgt ggacgcagat cgacatcacg cggccatcgt
     8641 cgcgcgcgta tggcgccatg gcctacgatc cggtccgcaa ggtcacggtg ctgttcggtg
     8701 gcgcgtcgaa gctagggacg ctgctgcgcg atacctggga gtacgacggc acgagctgga
     8761 agcagatcac gccgccgacg tcgccgacgg ggcgtgcgta ttcggcgatg gtgttcgatc
     8821 cgattcgcca gcgcatcacg cttgtcgacg gactcgacgg tgtcgatgtg ccggcgtcgg
     8881 ggctcgtcgc cgatgtgtgg caatacgacg gcacgacctg gacgcagctc acgacgaccg
     8941 gcacgccgcc accacgtcgt gcgcacgcgc tcgcatggga taccgcgcgt cagcggctcg
     9001 tgatgttcgg cggcattcgc cctatccccc ccaccacggc cgccacctcc gagacgtggg
     9061 agctgtcgtt cggcacgagc tggacgtgga catgcaccag cggcgaggcg acgtcgccaa
     9121 gcgcgtgccc ccaggcagtc acgcggccga cgaatcgcta cagctcctcg ctcgtgtact
     9181 cgccgcttca caacaccatc gtcatgtacg gcggcctgac aaacgccggc accgcgacgc
     9241 agaacgcgtc ggacacctgg acgtatggcg ccaccggttg gaccgccact acgcaggccg
     9301 tgtcgcccgg cggacgcagt gcggctgcgc tgagcgactg cgagatccag atcgatgcca
     9361 cgacattcca gcatcgtcct gtcctcatcg gcggctcgac cgatgaggga gcgaccgacg
     9421 aggtctggga gctgacctcg gcatggctac gcacgctcac gcccggcaac gcgccgccgc
     9481 gctacgacat cgctttgagc tacgacgcga agcgcgatcg caccgtcagt gtcggcggct
     9541 atctgccgcc gctcgcgcgc gccgacacgt tcgtgttcga cggcaaggtc tggaagcgcc
     9601 agccgccgct gccggcgatc cgattcgcga tgtcggcgac ctacgattcg acgcgcgagc
     9661 tcgtcggcgt gttcggcggg ctcacccaat cgagcaaccg tacgaacgag acgtacgcgc
     9721 tgaccggcct cacctggatg cagctgccgg gtcccgcacc atcgccgcgc tacggtgccg
     9781 cgcttgcaca cgatccgcgc gcgagcgtga acgtgctgtt cggcggcaac gacatgagcg
     9841 gcaccaacct gcgcgacaca tgggagctcg acatgaatgg ctggtcgcaa aacatgtcgg
     9901 ctggcggtcc gcccgcgcag ctaggcgcgg cgatggcgtt cgatcctgaa cgacaagagc
     9961 tcgtgctgtt cgacggtgcg gcgaagacgt ggctctacac gaatcgcgtg tggacgccgc
    10021 tcgagacgac cgcatcaccg cccgcacgta atggcgcgcg cctcgtgtat ttcccgtcgc
    10081 gcaagcggct cgtgttgtac ggaggcgtcg cggacaacgg cacgtcgaca acgctgctaa
    10141 cggatatgtg ggagctcgac accgacggcg acgcgcgcgc gtggcacgag gtcgtctacg
    10201 cgaatccgcc gccgccgcgc cgcgggtttg gctttgtcgc gcacgcgtca ctcgattcgc
    10261 tggtcttgcg cgcgggtcag acgagcagcg gcgaaccgct cgacgacacg tggctgttcc
    10321 ggtggcagtc cgcaaagtga acgtgcgcca ctaacgtacg agttaagatc tcagcgctgc
    10381 gggcagcaga tcagttacga tcgcgtccag atgcgcggga tgttgattgc gttcgtgtcg
    10441 ctcacggcct gcgcgtcggg aggcgacaag cccgatgcgg cgccggcgcg cgacactgcc
    10501 gtcgacgtgc cgaccgtcgt cagcgcggta tgtccgagcg ggcaactcgc agtcgacgtc
    10561 tcgtcgtcga aggagctgac ctgcgtgccg ctcgacggct tcacgcgatc cgcgctcgcc
    10621 gatcactgca gcgtgtatct cggctggcgc gatagctgcg atgcctgcac gacgccgccc
    10681 gcgaagtggg gccgcgtcga gactggtagt tgctcgcccg gcaccggcac gaacaacacg
    10741 tgcacgatgc cgacgctcgg cggcacaccg gtgcatatgt tcgggctcga tctcgacggc
    10801 gacatggacg gcaacgacaa gctgtacgcc ggccttcact gcacgagcga agacgacagt
    10861 ggcgcgatcg cgccgtgccc ggcaggccag ctcgtcaacg gcttcaacgg caccgcgtgg
    10921 agctgcgcgt cgtatgcggc ggccgtcgtc tcgtatgttc acgcgaactg ttcgatctat
    10981 cttggctggc aagacagctg cgatggctgc accagcgcgc cgccgaaatg gggcctcacg
    11041 tcgaacgata cgtgcatgaa cggcgcaggc accgacaaca cgtgcatcgc gccgacgcag
    11101 ctaggcctcg agcaagtgcg gctgttcggg ctcaacacgg acggtgacgt cgatggcaac
    11161 gacaagcttc acgtcgggct gcgctgcgag ccgccggggc agggcagctc gatccagact
    11221 tcgatgtgtc cgacgggaca gttcgtcgcc gagacgctca cgcaaggcgg ctttcgctgc
    11281 gagagtccgg ccgcaaccgt cgcgcagtat ttcggtagtc actgcacgct gtacttcggc
    11341 tggcatgaca gttgtgatgg ctgcatcacg ccgccgacga agtggggcac ggttcgcgtc
    11401 ggcgcgtgca tgaatggcac cggcgtcgac gacacgtgct cgaagttcac gctcggcggg
    11461 cagtccgtcg acatgttcgg gctgtcgccc gacggcaacg tcgacgagaa cgacgtgctc
    11521 tacgtcggtt tccgctgcga gtgactgtcg cggaacttgc tgtagttgtt ggcagatcgc
    11581 gaagcgtcgc gacctgggcc cgtgccgcgg ctctaccctg gtgatgactg cttactcgcc
    11641 gccttcttgc ggatcgctcg agtcgccctt cggttgcgcg ccgcgcgtcg tgcgcttggc
    11701 tttgggtttc ggcggctcgg cggcaggtgc ggcggctgcg gcggcgggcg cggcagcacc
    11761 gtcaccgccc gtcacctttg cgaacccgga tttctgggcg tcgtcgagat gctggcgaca
    11821 gccgtcgagc tcggcgtcac tctcgacggt cgcgaaacaa ctgcgcgctt cgtcggacca
    11881 cttgtcgccg gtacaccgtg agcgcagctg ggtcttggcg tctgcttgcg cgtcgggttg
    11941 gtcagcgtgg atgacgatcg cgagcttgtc cgcgacgacg tcgcacgacg gaccggcagt
    12001 tttcggaatc ggatccggag ttggcggcgg agccgcgggt ttgggcgagc caccacagga
    12061 ggcggcgatc gtgaggacga acaacgcgag gagtctcatc cacttcattg tagccggcct
    12121 cgtgtaagaa tcgggcgatg cgtcttttgg ggactgcgct tgtcgtcgct gtgtggctgt
    12181 gctccgtcgc ggaagcaaag ccgaagcaga cagctgcgcc caagccgacg cccgcggcga
    12241 ccgcgcccgg caagcccgcg aagcccggca agcaggcgac cccgcaacaa caggcggaga
    12301 tcgcgaagct cgaacaggag ctgacgcagc accagatcaa gcaggcgtac ttcgccgcgc
    12361 tcaaggtcgc gaagcagctc tgggacctac agaagaaagc gaccggcgag gactcgcccg
    12421 aagcgcagcg ccgcaaggct gcggtcgcaa gcgcgatgtc gaacgccggt gattacgccg
    12481 gcgcgcagaa gctgtacgag gagattctcc acacgaccga gaaggagaag ggaccggagt
    12541 cgcgcgaagc gctgtacgca ctgatgccgc tgaccagcac gtactggtcg cagagccggt
    12601 tcggcgaggt cgagccgctg atgaagcgac agctcgagct gaccaagaag gtcgagggcg
    12661 aaaagagccc gatgtattcg ggtcagttgc agatgtacgc cgggtttttg accctgcgga
    12721 acgaattctc ggccgcgcag cgcgtctacg aacaggtgct gcagctcgcc gagtcgaccg
    12781 cgacgagcaa gaccgacatt tcgctgctcg ggccggtcca ggcgcttgcg aacagtttct
    12841 ggcaaacgaa ccagcagcag aaagcgatcc agctgttcga gcgcgcgatc accatcgtcg
    12901 atgcgcagac cgtcaacggt ccgatcatga agatgagcac gcggtggggc atcgccgcgc
    12961 agtactcgta tggcggtcgc aaagatctcg ggttgccgct catgaagaag gcgatcgaga
    13021 tcggcgagaa ggacatcgct cagctcgaga aggacaagcc ggacgactat cagattccag
    13081 cgatgctcgg ccagatcggc tacatgtatc ggcagatcgg cgacctgccg cacgccgaga
    13141 aggcgtatct gaagacgatc gcgctcgccg agaagaagaa gacgtactcg ggctacgaag
    13201 catcgcttgc cgacctccgg cgcgaacagg gcaaaccgaa agaggcactc gcgctgctcg
    13261 aaaagggccg cgaccagatg gcgaagctct cgccgatctc ggcgacggtc tacaacacgc
    13321 agatcgcgca ggtgctgagc gagctcggcg agtacaagcg cgcgatgcag ctgctcgacg
    13381 agcaccgcaa gttcgtgatc aagcagtacg gcaagaagca cccgatgtac atcacggccc
    13441 agatgtcggc ggtgaacgtg tacgccgccg ccggtgagat caaagaggcg gagtcgatcc
    13501 tcgacgacgc gctcgacgcc gcggagcgcg atcttgcgaa cgtgatgcgc agcggcaccg
    13561 acgccgatca cgcgatctat ttcgcgcgta actcgtacat cctcgatacc gcgctgaact
    13621 tcggcttcaa ctacgcaccg aacagcggaa gcgcagcccg gctggcgctg accacgctgc
    13681 tgcgtcgcaa aggccgcatc ctcgatgcag cggctgcctc gctcgccacg atccgcagca
    13741 agctgtcgcc cgacgacaag aagatcctcg acgagctgaa cagcgcacgc gcacagctcg
    13801 cgaagctcgt gaatgcaggc cccggcgcga ccggcggcga tgccgacgcg tacggcaagg
    13861 aagtcgccgc actcgaagag aaggtgctca agctcgagat cgcggtctcg aagaagagcg
    13921 ccgcctatcg cacggtgaca cagccgatcg agctggctgc aatccagaag gtgattccga
    13981 gcgacgcgcg gctcatcgag atcgtgaact atcagccgtg ggatccgcgg atggcgtaca
    14041 cgatctcgcc gaagctccag ccgcgccact acgccgcgta cgtcctaggt gcgaagggcg
    14101 acccggcggt catcgagctc ggcgaggcgg cagcgatcga caaggcgatc gaagacttcc
    14161 gcaaggcagt cgcggatccg gacaacgatc gcgcaggtga cctcggcaac gcgctgttca
    14221 agctcacgat gggcaagatc gttccgaagc tcggcggcgc gacgaacatc ctcgtcgcac
    14281 ccgacggtgc gctcaacgtc gtgccgttct cggcgctcgt cgacgataac aagcagttcc
    14341 tcatcaagaa gtacacgttc acgtacctga cgagcggtcg cgacctgttg cgcgtgggct
    14401 tgaagacgaa agcgcagggc ggcggcgtgg tgttcgccga tccgaagttc gactcgaccg
    14461 gtcccgcggc tccgggaggc ggcacgacgc gcggcgcgcg ctccgtcgat ctgcgttcgc
    14521 tgcgctgggt accgctgccg ggcaccggcc aggaagccga cgcgctgatc aaggcgatga
    14581 agaacctcaa ggtctatcgc ggcgatcaag cgaccgaggg cgcgctcaag ggcctccacg
    14641 cgccgaagat cctccacctc gcgacgcacg gcttcttcct ccccgacgaa gcaccgccgc
    14701 cggcaccgga ccagagccaa ggcggaccgc cgatgggatt ggcgatgccc ggcagcgcgg
    14761 tgaacacatc cgtgggctcg acctacgaga acccgctgct gcgttcgggc ctcgcgctcg
    14821 ccggcgcgaa caagctgcag agcggcgacg acgacggcgt gctcaccgcg ctcgaagcca
    14881 gcggcctcga tctctggggc acgaagctcg tcgtgctatc ggcatgcgag accggcgttg
    14941 gcaaggtgag caacggtgac ggcgtctacg gcctccgtcg ctcgctcgtg atcgccggcg
    15001 ccgagagcct cgtcatgacg ctgtggcagg tcgacgactt cgcgacgaag gacctcatgg
    15061 ccggcttcta caagaagctg gctgctggca agccgcgcag cgctgcactc cgcgagatgc
    15121 agctcgagat cgcggggcaa gagaagtacg cgcatccgtt ttactgggcc agctttttgc
    15181 cggccggtgc actcactccg atcaaggact gacccatgtc actacgcccg ctgttgatcc
    15241 tgtgtctcct caccgcgacc gtcgccgctg ggcccaagcc caagaaggcg aaacagccgc
    15301 cggtgaagtc gcccgccgcc aagctcgaca agaagcagct cgagatcgag cggcacgagt
    15361 ggatggcgca gtactacatc ctccgcgcga acgacatgaa gggcgccgcg aaggagtacc
    15421 aggcgatcct caagctcgac gcgaaaaatc tgaacgcgtc gctcgcgctg tcgtcgatct
    15481 atcgccgcga caagcaggac aagcaagcgc tcgacgtgct cgtcaaagcg acgaaggcaa
    15541 acccggcgag catcgatgcg tggctctcgc tcggcgagct gcagtcgcag atgaacgaca
    15601 gcaaaggcat gaaggcgtcg gtcgacaagg cgatcgcgct cgacggttgg aacacgagcg
    15661 catactggct cttgttcgac gatgcgagcc ggcgcctcaa gggcggcgag gctgctgcca
    15721 agaccgaagc gctcgctgct gcgcgcaaga tcatggagac cacgccgccg cgccgccgca
    15781 ccggcacgct gtacaaggtg gctgagcgtg cggtcgtcga gctgtcgggt gagccgatcg
    15841 agctgacgat ctacgacgcg aaggccgcct acgccgccgc gttcgagacc gggctcatgg
    15901 gcacgatcaa ccagcagatg gacaaggcgc ggcgcggctt cgacgagtgc acgaagagcc
    15961 agccaaagaa cgaggagtgc cactacttcc tcggcctcgt ctattcgtcg gtgaagtcat
    16021 ccgacagcta cgatccgaag aaggcgctcg cccaattcgc agcagcaccg acgacgccgc
    16081 tcgcgtgggt cgagaccgcg cggatccagc gctccaccga caagaacggc gacgcgcgta
    16141 cctcgctcga gaaggcgctt tcgctcgatg gcgatctcgc agtcgcccat atcgagctcg
    16201 gcatcctcga caagctcgac ggcaagctcg acaacgcggt caaccacttc atcgccgcga
    16261 tggacgcgga cccgtacggt gcgaccggtt cgcgcgcact caccgagctc acgaaggcca
    16321 agccgacgca cccgcgcgtc acagaaggca tgctgagcgg caagaacatc gacgtgttct
    16381 cgaccgagcg ctacgcgtcg atcgtgcacg agatcgagaa ggagctcggt ggcgtcgacc
    16441 cgaaggcacc cgagaaggcg atgctcgagg acgtcgtgcg ccggctcgcc gatggcagcg
    16501 gcatcaagat gcagttcaag gtgaacttgc tcaacaccga catggtcaac gcgttcgcgc
    16561 tcgcctcggg cgacgtctac gtcacgcgcg gcatgctcga cctgatcgcg aagaagaccg
    16621 gcaagaagcc ggacgcgaac aacgacgcgc tcggccacat tctcgcgcac gagctgcagc
    16681 acgtgatccg caagcacacg atcaacacgg ctgtgttcca ggaagccgtc aaggacggcg
    16741 gccgctacct cgacgcgtcg atcctcacgc acgtcgagcg cattcacgag atcgacgccg
    16801 accgcgaagg catggtgatg gcgttcctcg ccggctacca cccgcgcggt ggcatcgagc
    16861 tgatggagac gatgggcaag gagatggaga ttcccaagaa cctcgatcac ccgacgttcc
    16921 aggagcgcgt cgagtacctc accgagtatt ggacgaacga cgtgcgctac gcgttcgtgt
    16981 cgttcaagct cggggtcggc gcgatggatc gcggcgcgaa gctcgaggcc accgacatga
    17041 agaaagcggt cgaggcctac gaggaagcgg cggatgactt taagcgcttc cgcgcgatgc
    17101 taccgaccgt gaaggaagcc gcgaacgacc tcggcatcgc atacaccaag ctcggtgtgc
    17161 tcgcgatgaa caaggacgag tcgccgctcg gccgctggca gagccggttc tcgctcgagc
    17221 gcgaatcgag cgtgaagtac gtcaacctcg cgcgcgacga ggagaagacg agcacgcgcg
    17281 gcaccgagaa gaaccgtatg ccgtggcagc tgcgcgaagc gatcgccgcg ttcaaggacg
    17341 ggctcgcgat cgacgagggc tacaacaagg cgcgcctcaa cctggctgcc gcgtacatcg
    17401 cgggcaatca gatcgacaac gcgaacgcga tgatcgcgaa ggtctccgct tccggcggcg
    17461 tcaccgacgg tgacatcgag ctcatccgcg gcatcgcgct cgccgagggc aaggactacg
    17521 acaaggcgaa ggcgtcgttc gagaaggcac tcggctcagc agccgcgaag cgcgcggcga
    17581 cgtacaacat cgccaagacg ctcgacctcg ccggcaagaa ggacgaggcg aagaaagcgt
    17641 gggggcagta cttgaagctg tacccaggcg ggccgtgggc tgcggctgca accgctgctt
    17701 ctgcgaagct gtgatcccgt agctctacgt attgtcgcga tcgatcgccc gatggtttcg
    17761 tgacgcctca cccattcggg agaggaaaac atgttgggcg aaaagttcac gcaggcgcag
    17821 ggcaaggtca cggggacgcg cgtcattcca ggcatcgacg gcggcccgcc gctggtcgaa
    17881 acgtcgtttc acgtgcaggg caaggtgtac ggcgtcgatg tcaccgacat cggcacgtac
    17941 acgtcacgga tggtgccggg cggcacgctc gtcggtgaag gtcagggcgt ctcgatgacg
    18001 cccgacggcg actcgatcgt gtggaagggc ttcggcgtcg gcaaaccgac gggcaggggg
    18061 ctcgccgcgc agtatcgcta ctcgatgagc tttcagacca cgtcgcagaa actctccaag
    18121 ctcaacggcc tcctcgctgt tggcgaatgg gaagtcgacg agaacggcaa ctgcaagggg
    18181 caaggctggg agtggaagta ggcacgtgtc gccgcccctg acggggtaca atcgttaggg
    18241 gtgacgaccg tctcgacgac accaggccct gctgcgatcg gctcggacga caaggagctg
    18301 atttcagggc aagaagttgg cgagtaccgc atcgagggtg tactcggccg cggcgggttc
    18361 ggcaccgtgt acggcgcggt gcagccggtg atcggcaagc gcgtcgcgat aaaggtgctc
    18421 tcgcgcaagt acgccgcgga tcaagacatc gtgtcgcggt tcgcggccga ggcgcgcgcg
    18481 gtcaaccaga tccggcaccg caacatcatc gacatcttct ctttcgggca actccccgac
    18541 ggccgtcact actacgtgat ggagtatctc gacggcgagc cgctcgactc gcacttgcgc
    18601 acgtacggcg cgatggcgct cgaggaggcg attccgatcc tgcgtgcgat cgcgcgcgca
    18661 ctcgatgccg cgcacgccaa aggcatcgcg caccgcgacc tcaagcccga gaacatcttc
    18721 ctcgcgaatg atcccgacgg cggcgtttat ccgaagctgc tcgacttcgg catcgcgaag
    18781 ctgctcgcgc cggaagagac cacgatgcat cgcaccggga cgggcgttcc gatcggcacg
    18841 ccgtattaca tgtctcccga gcagtgccgc gggcgcgacg tcgatcaccg gaccgacatc
    18901 tactcgttcg gcattgtcgt ctaccgcatg ctcaccggcg cgctgccgtt cgaagccccc
    18961 gattacgtcg agctgctcta caagcaggtc agcgaggagc cggcgctacc gtcgcgtgtc
    19021 aatcccaagc tgcccgagag cgtcgacatc gcgatcgcat ggatgatgca gaaggatcgc
    19081 gagaagcggc cgaagaccgt gctcgaaggc gtcaacgcgc tcgatccggc gaacccgatg
    19141 cgaccgacgc cgctgccgcg ctcgcaccgt ccatccgtcg gtccgaccgg cgcgacgggc
    19201 cgcccgcagc agacgccgag cgagatcgcg ttcgccgaga ccggtgtgtc gtcgccgaac
    19261 gcgaacgcag cgaccggtgc gcagacgtcg gcgccaccga atccgcgggg cactcggatc
    19321 acgatggtca tcgtcgccgg gctcgtggtc gcgggcatcg gtgtcggcgt ctacatgaag
    19381 acgcggccgc ctcacgtcac ggtcgtcgag cgcgacaagg gaccgaccgg atccgcggtg
    19441 ccggcccagg agccgccaca tgagccggtg aagcagccga tcgccgagcc cgccgatgcg
    19501 gcagcgacgc ccgaaaccgt gatgctcgag ctcaccggtg cgcccgaggg tacggaggtg
    19561 ttgcgcgacg gcaagctgat cggcgtcgtg ccgaaggtgc cgctgccgta cagcacgaag
    19621 gtgcaatatc tcgtgctgca agcggacggc tttcagccgc tgccgatcac cgtccttccg
    19681 gatcaagatc gcaggatggc ggtgaagctc aagccgaaga agcgcgctgg cggctcgcga
    19741 ccgagtcacg acgacgtgga ggacccattc aagaaagccg attgatgcgc gcattcgcga
    19801 tcgccgtcat cgccgtcatc gccgccggct gcgccgatct gcctgcgttc gaagaaacat
    19861 gcggcaacgg cgtcatcgat ccagacgagg actgtgacaa caacgggccg acctgtgact
    19921 cacaatgccg cgtgacgtgc gcgaacaccg tcgattgcga tgcactcccc ggcgcgtacg
    19981 tatgtggcgc ggactcgatc tgccacgcgc cgagcggcgt cttcgtgcag accccggcgg
    20041 tcgcatttcc attcgtcgtc gggcagggcg cggtcgccga tctcgacggc gatcgcatcg
    20101 gtgacgtcgt cggcttctcc gatgtcacga tcgacgtgcg gttcggcgat ctcgacgctg
    20161 atctcgggac gcgctcgacg atgctgctgc cgtactcgaa cacctcgatc gcgatccgcc
    20221 ggttcgacga cgcgaccggt cattccgaca tcgtcgtgcc gacgcccgac ggcctcgccg
    20281 cgtacaccgc ggccagtaag cagctcgtct cgtacccgtt ctcgagcgcg cccggcgcga
    20341 gtggcgattg cgtgacgctg ggacagaccg ccgacccggt cgcgacgttc gagctcgaca
    20401 agcaccggct cgcatttgtc gcgcgcgtgt tcgcgaccgg cgaggcgcgt gtcggcgtgc
    20461 tcgatgtcgt gactggcgag tgcaagtcgg ggccactctg caacctgacg ctgccggatg
    20521 cgcaacagct cgccggccgg ttgttcaccg atcactacga gacccaggcg tcaccgctca
    20581 cgtcgacgat cgcggtcggg ttcgcatcgt cgatgacgag cgagcaagtc tgtgcgctgc
    20641 gcttcaccgg cacgcccggc aacttcacgt tcacggcgct caccgcgacg ccgctcgtgg
    20701 ccgaacccgg accgatcgtg ctcgcacgcg cgcagcccgc cgccggctgt cctacgctgt
    20761 tcaccggcgg aggtggtggc atcgagcttt atgcggccgc cggcatcccg gctgcgtgct
    20821 cgctcgcgac gttgccgagc gcgctgccgg gcttcggtgc gaacgtgctc ccgaccggcc
    20881 ggattccgct cgtgcccgcg atcgcaggcg ctgcgcccga cgcgctcgtc gtcgcgaagg
    20941 gcgtcggatt tgcagcgtcg ggcgacatct atacgctcgc ggtcccgacg accacgctga
    21001 tgtatcactc gccgcggccg ctctttccgg tcaccgcggc ggatctcgac ggcgatggca
    21061 gcatcgaagc cgttgcgagc gtgctcggtg ccggtgggcc acaaggccag ccggacctcg
    21121 acgtcctcta tcgcaccgcg atgccggtcg acagcttcgt gccgtaccgg atcacgacgc
    21181 agggcgtgcc gacgaatctc aagctcggcg acttcgacgg caacgcgctc gccgatatcg
    21241 cgtacaccga gcgcatcggc accgtcgagc gtctgatggt cgcgtacggc acgcgcgatc
    21301 gtccgctgcc tgccgtgcag atcacggcgt tcgatcaaat cgttggcttc gcgaagctcg
    21361 atttgccgga ctctatcgag ccgaccgggc aagtgctcga cgatctcgtc gtcatcaatc
    21421 tgccgccgac cgcgatgccg accatgacga tcctgcacgg caacccgcag cgctcgatgc
    21481 tggcgtactt cgatccgcgc gcgatgttca ccgcaaccga ctcgtcgttc gtcgcagcgc
    21541 tcaccggtaa cttcgacgcg acgcctggcg tcgacgtgct cgcgttcgag cgttacacgc
    21601 cgacggcggg cagcgaagca gtgcggatgt ggctcgcaca gggaacggtc ggcagccagc
    21661 ggctcaccgg tgcggggcaa ccaatcacga cgtcgctcgt caccgactgc acgcaagccg
    21721 gtccgttgtg cctcaccggc gcgcgcttcc tcacgtggcc gctcggcgac cgcgatgtcg
    21781 tgatcgctgc cgatgcccag atgcggcccg gtaggtcgat cgtcacgatc gatccgatgg
    21841 ggcccgtgac gcctgcaacc gatccgtcga cggcgctgcc gagcgagccg ttcgtgttcg
    21901 ccatgtgggg cgccgagctc atggccaccg gacagcacaa actgctcctg tcgctcggcg
    21961 gcattcgagg cctcggcggc actgccaagc aggtgctcga ctgcaccgtc gacatgcgtg
    22021 gcaccgtgtc gtcgtgcgtc gagtttggcg gcgaggttcc cgagctcgcg ggctggacgt
    22081 gtcagtacgc ggagcccggt cgcgtcgcac cgcgcggccg ccatgatccg ccgccgcggt
    22141 cagccgccgg cagcgtgctc gcgctgtgta agaaggaggg ggacggcgga cgcgtggtcc
    22201 gcatctcctt cggcgacggc agctatcacg ccgagccggt gctcgatcac gcggagaact
    22261 acgagttctt gtcgatcggg gacatcaacg gtgactcgct ggacgatgtc gccggcatcg
    22321 cattcgacga gtcgttcacg ccgttcgtgc agattcacct gcagtgcgac gcgcgcgacg
    22381 tccagtgccg caacaccaac gcaggagcgc cgctatgatg cggatcgcac tcgcgctcgt
    22441 catgctgtgc gctgcaaccg cgcacgccga tcgcaagaac gccgaacagc tgttccgtcg
    22501 cggcgagaag gcctatcgcg cacagcgctt cctcgcggcg gcgcaggcat tcgaggccgc
    22561 atataaggag ctggccgcgc ccgagctcgc gttctcggcg gcccaggcgt atcgccgcca
    22621 gtatcgcgtc gatcccgatc atcccgagta cgtcgagctc gcggtgaagt actacgagat
    22681 ctatctgcgc gaggtgaaga gcggcggccg caccggcgat gccgccgaca gcctcgacga
    22741 catgaaggcc gagctgcgcc atctcgagaa gaccggcaag ctcggaaagg tcacggccga
    22801 gacggtgccg atgctcgcgg tcagcgtcga cttcgccgat cgccagcagg ctccgacgaa
    22861 gctccacgag atcgacgaca cgcggctgac ccaggcagag acgcccgtga acgcgttcgt
    22921 cgacgggaag accgtggatc ccgacgtgct tctacccgtg aaagaaggcg agcacgtcgt
    22981 gcgcgccgaa gccgacggct acaaaccggc cgagaaacgc gtgcgcgtga tcgcaggcgt
    23041 tccggcgtac gagcagctca cgctcgagcc gctgcccgcg cagctcgatg tgaagaccga
    23101 ggcaggcgcg accgtgatcg tcgatggtcg cggtgtgggc acgacgccgc tcgccgcgat
    23161 ctcgctggcg ggcggcaccc acgtcgtcac cgtcgtgcgc acgggccgca aaccggcggc
    23221 gcgcgagatc cacgtcgaac gtgcgcagac gacgagcgtg tcgatcgagc tcgaaccgac
    23281 ggtgcgccgc cgtgcggtgc cgtgggtgct cgccggcgca ggcgtgctca cgctcggtgc
    23341 gggactgtcc gcggtcggtg ccgcctactg gaacagcaaa gcggtcgacc ggcgcgatgc
    23401 cctgatcgcc ggcgatcagg atccgagcgt gctcgccgat tacaacgacg atcgcgaaaa
    23461 ccgcgatcgc gcgatcaccg gcgtatgggt cgccggaggt gcagccgttg cggtgggcct
    23521 gaccgcggcg tggctgtact acttcgactc gccctccgcg gagggtgttc gcgtggtccc
    23581 ggttgcctcg ggtagcgcgg gaggcgctgt catttcaggg cacttctgag gtcttggcgg
    23641 cggtaggatc tagggctata agccggcacg acatgggttc gacgggtaag aggaatctgg
    23701 ctgcggtagc aacgacgctg accttcgaga agttctggca gtggcttggt ggtcacgcga
    23761 actgcatcgt gcgcgcaggc accccggaag tcgttctcat cgatcacgac gactttcact
    23821 ggaccctgat tagcgaggac gaccacacca aggttgtcca gctggcgcgg gcgaaagatc
    23881 tcgtgggcga gatcctcgtg ttttcggccg aggtcgccta cgtgcagtgc gagcccagcg
    23941 agtccgaagg ggaatggctc ttcgagtgca tcgtggagtc ggagaaggct cgtgaagtcg
    24001 cttatcactt cgtgatggcg cacgaatacg acgacggcga gcatcgacgc gaagagaagt
    24061 ggacccacta gcacccgtgc tagattcacg gggctggtat gcagcccgtt gaggcctctg
    24121 atcgcgtcgg cagcacaatc gacgggcgtt acaaaatcac cgaggtgatg gcggccggct
    24181 cgatgggcgc ggtctttaag gccgagcgcg ttcctgtcgg caagctcgtc gcggtgaagt
    24241 tcctccacgc gtcgtacgcc aacgacagcg agttccaggc tcgcttcgat cgcgagacgc
    24301 gggtgatgag caggctcaat cacccgaact gcgtgtccgt cgtcgacttc ggcgtgtggg
    24361 aggccgagcc gtacctggtg atggatttcg tcgacggcgt gacgctgcgc tcgatcatgg
    24421 acaaaggtcc attgccgccg aatcacgcgc tgcagatcgc gcggcagatc ctctccggcc
    24481 tcgcgcacgc gcacaaacag gaaatcttcc accgcgacgt gaagcccgcg aacgtgatga
    24541 tcagcgaaga gatcggtcac ggccagcgcg tgcgcatcct cgacttcggt ctcgcgcgcc
    24601 tgcagggcaa cgtcggtcgc gacgcgacgc agacgaacat ggtcgtcggc acgccgaact
    24661 acatggcacc cgagcaaacg gtgcccggtt cgacggtcga cgcgcgcacc gacctctacg
    24721 ccgtcggcgt catca
//
LOCUS       DASUFX010000136        19465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_578007,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000136 DASUFX010000000
VERSION     DASUFX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19465
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578007"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFV99_05370"
     CDS             <1..453
                     /locus_tag="VFV99_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058768.1"
                     /translation="KPDTTKPDTTKPDTTKPDTTKPDATKPNRYDLVQKVEPGRPRSK
                     LVPILVGVGGLALVGGSVGFMVWAGSTYEKAQNEGNDDKQLDLWHSANTRRYVAEGLG
                     AAGIAAIGVSVWLYLRSGSEPQRTIDHPAMIVPAVDGERATFVVSGRF"
     gene            546..2696
                     /locus_tag="VFV99_05375"
     CDS             546..2696
                     /locus_tag="VFV99_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058769.1"
                     /translation="MFATDQPFAFAGDEVWLEGTFEDGALVNFAGASAPIPLNVRYEH
                     RAAVTVPADATTGALSIQTSGGIDGKVPFRGLTFKPGLQPFMPHNDQNDYARLNYLDR
                     VHVGGTAETIGNRLYLIGGKVDVVVTADVESAAIGLDGGMGRPKMFESLQTARAYHSS
                     IRVGRYIYVIGGLGSGSLRSVERAQIGDDDSLGPFETVPGVELVEGRYGHKTTIIGNY
                     VYAIGGRSVSELATVERAPIYENDELGTFEPISDVALKTPRSHFTIVKGATYLYAIGG
                     LHNGIPITSVEKIEMFGNGYVSDFQEAGALREPRGNHGGIVVGNNIYVFGGAGLTGPL
                     ASVERAPFSEDGTLEFFGEFNHVLTEAKQDSATAVIGNYVYVIGGERGGNAPALLVER
                     AGLFDYGALDTFANAPVSLNQPRSQAAVVVIGKWLYVIGGVNGTGALNTTEVAEIKPD
                     GSLSDFGIVQAPGLGTARYGHRALINDNVLYILGGLDTAGNPLKTIEAAAINPDGSLA
                     NWGYHGTQMFTARGRFSLFTSGITVYGLGGYTSATQPTATATVEYASVNAGTQFGSFN
                     DLSVTTLDDARDGAAMVAVGRYLYVIGGDASAGSQVARALTKPLGTGFTGPFTPAGDP
                     LTTARSGHTVTRLGHSFYVIGGADTSVTVERAMVDDVESNVFGFSPSSTVQLMTNRSH
                     HATVTLGNYVYLIGGKAEPANGSYLYSLERAAIR"
     gene            2702..3301
                     /locus_tag="VFV99_05380"
     CDS             2702..3301
                     /locus_tag="VFV99_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058770.1"
                     /translation="MKRVVALLVAFGLVAALIAWRMWPHGETHAAAPSSPSSATQPGA
                     TSTAQSSRVRKLSPEQRRELGDKIAAAIERARTAQAASTSHGTSAGSAPALPEPPSIP
                     LEEAAKPLQEALQDAIPLLADCFEQHADNRAAAMMTMTSDPDLGTVIDTAAITDKDGK
                     PLEPKVDECLRNAIDSLALPPLGAQAGLLKVQYTFRFDE"
     gene            3311..3778
                     /locus_tag="VFV99_05385"
     CDS             3311..3778
                     /locus_tag="VFV99_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058771.1"
                     /translation="MRLALFVLLILAAPVHAEPTKVKSTALTLGKVKAKPARLLANRV
                     TVDVTRSVTPCILKLVVEPQHPLFKRFAIELGFEVNATGAIKNGSGPLTAEIKVAGAS
                     APFVAKTGRVDVTRKKSHDFDAKLMLEFEHEAVPWTLSGTVRIENAACLWDVE"
     gene            3964..4683
                     /locus_tag="VFV99_05390"
     CDS             3964..4683
                     /locus_tag="VFV99_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058772.1"
                     /translation="MRTTSSLLFVALLSASSSAFAQPAVRDHRDPAPVAAAAPASAPE
                     VRDHRDNSIEGRPRYRRRPGPRFMMPLKIDIGAAGANTSRGFAPGMSAAVGIHWASLS
                     PRPTDTDVGIGLFGGILAAPEDTSVMNDKNGVAYGGAYLELAHTLSQGTWWRTWASGR
                     GEYLGSTAFDQDHAGFGASGRLSAELYVSGVGIEPRGVFLGTYAIGVFVEAGVRDMVE
                     DVGKFQMSGGLTFRTPLVFAP"
     gene            4773..5318
                     /locus_tag="VFV99_05395"
     CDS             4773..5318
                     /locus_tag="VFV99_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058773.1"
                     /translation="MLNWFLRKRLTAFEKDFGYDVSYMRHVLDVDMRAFLRFARAASA
                     VKYRKDVPMDLYHAVGFTGIVAADCGPCTQLGVTMALREGLPAATIAKILRGNLDDMT
                     EPTQLGVRFARAVIARSPEADELREEIVRRHGPRAVISLGFALVASQMYPMFKYALGY
                     GHACQRIEVAGTTVTPKLAVA"
     gene            5315..6145
                     /locus_tag="VFV99_05400"
     CDS             5315..6145
                     /locus_tag="VFV99_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058774.1"
                     /translation="MSDFEEHRQFLIGVAYRMLGSFAEAEDIVQDAFLRWSNTDQAAV
                     EHPRAYLARVVSRLCLDRMKSAAARREQYVGTWLPEPMVAEPNEPFADDLSLALLLTL
                     ERLSPLERAAFLLHDVFDMDYSAIADTLERSEAAVRQLATRAREHVREERPRFAATDE
                     TRARLTDAFQHAIADGDIATLARLLADDAVFYSDGGGKRLAALNPIYGKEKIVRFFIG
                     ISSKRPVIERVDHVQINGLPGFVFHTDEGVETLAFDIAGEQVVAIYAIRNPDKLRHLA
                     "
     gene            complement(6142..9144)
                     /locus_tag="VFV99_05405"
     CDS             complement(6142..9144)
                     /locus_tag="VFV99_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5058775.1"
                     /translation="MRERRRATDTKAGLEIRLFGELVVARDGQPLTLPASKKTRALLA
                     YLAATGKPQLRERLCTLLWDGPDDPRAALRWSLTKLRPLVDDDGTPRLVADRDRVELV
                     PGDAAIDLVTLRAAIPGGTSAGVARASTEALVAVAPLVRGELLEGLDLPDCFRYDEWL
                     RAEREAARRLGVTVLSALVERLRDTPADALDHARTWLALDPLDEAAHAAVIRVLVELD
                     RKTEALAQYASCARLLERELGRGPSRELERLRIVIGSQTAPVPAPVATPVPALIAEAR
                     GQPRVALVGRASECATLAALLDQPRTRVVLLIGDPGMGKSRLLGELDTLAHARSIELL
                     RGRGVEAEQVRPYGAWLDAFETASVANHPFKAANVSETDRARLFESVVEWLVDRTHGG
                     TLVLVFDDVQWIDEATAALIHYVARSPRTTQIRIALAARAGELADNASALRLVRGLTR
                     EGAVQQIGLSPLDASETAALALAHAPGVDAARVFAESGGHPLFAVEIARALARGDTTW
                     ASLESLLVERLELVEGTARELLPWAAALGTAFSADVLGTITGLPLVELARAFEELERR
                     AILSANAAEWDFAHDLVRAAAYHQVSEPRRRLLHLQIARALAALPDPDGDRSGQIAHH
                     AGLGDDAALCATACLAAAERAMRLFAPEEAAALAERGLGHAVRLVGHERVRLQIGLLV
                     TALHADVRRQRRPAIAKALERAIVDAQIAGCAAEAARGLNELAHIPFGSGDYGRARSM
                     SLEATERARNAAPDDRARTLAYSAQCLALVGREIAEAEQLANEAAALLSQKPVDIPTL
                     PFAFAFIRMHQGDDDAAITLLRRGFELAERAGMWWLCALGWARAALIEVRRGRPVDAL
                     PYCAALRANAVRIGEAGDIPFGAVLEAIARQDLDEPVELEPAIEALRAIDAPNLLALA
                     LVVIAERELVRGDLLQAARRSDEAVASALRSQHENTIALAHCVAAQIAAADGRLSDVR
                     KHVDAVSAIDTAILSTRATAAVHALPRR"
     gene            complement(9157..10224)
                     /locus_tag="VFV99_05410"
     CDS             complement(9157..10224)
                     /locus_tag="VFV99_05410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5058776.1"
                     /translation="MTTKNVDDGKLNEFLGKVVTDVGGAMSAALVVIGDKLGLWKALA
                     RAEAPLSPSELAKRTETSERYIQEWLNAMAAGGYVTYSKDTLTYQLEPEQAVALADSE
                     SPAFVPGLFQVTAAMWAGESKIAANFRTGAGLEWGHQHPCLFEGTERFFRSGYLGNLI
                     QAWLPALDGVVAKLQRGAKVADVGCGVGASTIIMAKAYPKSTFVGFDYHAASIELARQ
                     RAREAGVADRVTFEVARATDFPGRDYDLIAHFDCLHDLEDPYGAAKRAREAIAPDGTW
                     LIVEPFAGDRPEQNHNPVGRVFYSASTMLCVPHSLSQGGPALGAQAGEARLRDVATRG
                     GWKAFRRATETPFNLVLEARP"
     gene            10299..10547
                     /locus_tag="VFV99_05415"
     CDS             10299..10547
                     /locus_tag="VFV99_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX5058777.1"
                     /translation="MPIFVVERVFSPPITEAQFYAAGEVLEKCIKERDIKWLSSHFSA
                     DGSRSICMYDAADAERVREANRTAGAPFERVWQARSMG"
     gene            complement(10605..11474)
                     /locus_tag="VFV99_05420"
     CDS             complement(10605..11474)
                     /locus_tag="VFV99_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5058778.1"
                     /translation="MKTLAIIAATIALFHVSTAAAEPDAKSTYKDIEKTLGFVPQFFK
                     AFPEEGIAAAWDELKSVQLNPKTALPGKTKELIGLGVAAQIPCKYCIYFHTQIAKLNG
                     ATDREISEAIAMAGATRHWSTVANGLQIDFTEFKQEVGRVFAYVKNPTPKGDAVAVTD
                     AASAYKDIERTLGSVPTFLRRFPEVGIASAWRMMKTVQLNPSTALPGKTKELLGLAVA
                     AQIPCQYCIYFHTEAAKLNGATDAEIREAVAIGAITRNWSTVLNGSQADESQFRKDVD
                     HVVANVKKAAAKK"
     gene            11560..13458
                     /locus_tag="VFV99_05425"
     CDS             11560..13458
                     /locus_tag="VFV99_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058779.1"
                     /translation="MRSLATMVGLGLVAACGDNLRPADNDIRGPYVLLDFTRPTLFSA
                     PVPAWDLTTEELAGIANPLDSAMVEQARELLANTRGFSRAGGVFFQISEPLAVDELPG
                     IAETVAADSPVMLVDVDPLSPELGRRIPLEVSFRTDASGYGAPNLLALVPLQGVPLHR
                     RTRYVAIVTTALHAASGPALLPAPASVLDNFRAVIAAAGVPETAVAGVTAFDTGDPTA
                     GLAAARTAALALPLPTLASAPTQTDLFSDFCVFQSTIGMPDWQSGAPPFATDGGTWEL
                     DANGAPIVQRTEVAKVVITLPRTAKPTAGYPLVVFVRTGAGGDRPLVDRGQQSMDGGP
                     PIVVGEGPARYLARAGFAAIQVDGPLGGLRNPTSGDEQFLTLNLENLGALRDNLREAA
                     VELDVIAHVATTLHVSGTSCPGAASDATFDAAHFAIMGHGMGAWIAQLAAAYEPLFGA
                     QILSGAGASWIATLLNQQKPLALRPLVASLLREQTIDSADPILTIVQWALEPADPQVY
                     ASSARPVLQIEGVVDSFAPPRMANASSLALRLDLAGSSLDDAADPRLVGQLPLVPLLA
                     LVGRSQVALPATGSVIAQFAEDGVEDGHEVLYQVDSPKHGYRCFLASWLTGTPTLPVA
                     ASRDAPCP"
     gene            13665..15416
                     /locus_tag="VFV99_05430"
     CDS             13665..15416
                     /locus_tag="VFV99_05430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058780.1"
                     /translation="MRCASCGAKTTLEQLTCPECGAPQRRSGVRSAGSVLGSYRLIEV
                     LGEGGMGVVYLAEHTRLGRRVALKTLRTDFAENPMAVRRFFGEARAVNKIRHQNIVEI
                     TDFVEEAGGDNYYIMELLRGKPLSNVIDNEGVQPLARTVNIMAQVARALQAVHDAGII
                     HRDLKPDNVFLIERGGQQDFVKLLDFGVAKLIDAVDSIGVSSTAAGAILGTPEYMSPE
                     QASGKTVDHRTDIYSFGIMLYELVTGQLPLRGDNFGELVVKHLTITPTAPSRVPDLPH
                     EVPAELEDAILACMEKNEDLRPDRIDIVGDQLEAIAAAAGWELPTYTAVPRATGRMTV
                     MSAQTGSGPVSGPVSIAPLRRTPPKGVPALGHAPTLEIAPTGSGEMPALRAPSGELRS
                     PSGELPSAASSVVLPTAPPAKRWPLYAAAAAVAVSAIAFLIVNLRGNDKPAPQAAPPV
                     AAPTKPTAPVNETIEIEIVTTPASAEVVRASDGHVLGKTPLVLPFARADRAEMVEIRL
                     AGYVAQKQSLRLDRGSRISTVLVPVPAETVAKPSRSRGKPTKKAQPTATGTPTRPAEP
                     AKGSANVDRSGVMDPFK"
     gene            15424..16041
                     /locus_tag="VFV99_05435"
     CDS             15424..16041
                     /locus_tag="VFV99_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058781.1"
                     /translation="MTVLVRALAIVCMLTAVGAAQPAPPPADPKVEAKRLFDEATEHY
                     KLAEFTQALAGYEKAYALFKAPAFLFNIAQCHFQLQTWDRAIFFFESYLREQPDASNR
                     TLVEDLMREARERQAAARQAEQRKLDLEKERLELEKRESEHLAKELELQTASLVAPTE
                     PPVYKKWWFWSIVGGVAVATIATTVAVTTRDTVLPSGSLGTVDQR"
     gene            16041..>19465
                     /locus_tag="VFV99_05440"
     CDS             16041..>19465
                     /locus_tag="VFV99_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058782.1"
                     /translation="MRTLALLTAVGLGLAGCADKVQTGITGVEITVTYPTDRNIDQLY
                     AWGIADGETPLEPTIVLEHPRKLTSGRDSFVVLVDNSVAGREMIVRVDGLAGSTVVAS
                     GLEVVTLTGGKLTHITVMLGAAAVCGDGVIAAGVEGCDDGDQDDGDGCAALCFVESGY
                     SCNSEPSRCTNGIACNDGVDNDGDGLIDTSDPGCADTMDTSESGSNACDDGIDNDKDG
                     KIDYRADSTGDDGCASLTGTTELNGGVCNNGTDDDGDGLIDYQTGSLGDPGCTSVMDL
                     SEHGAASCDNGADDDGDGVTDYPMDPGCTGAADTDEHGSLACDDGTDNDSDGRTDFLP
                     GGAGDPGCSSPSDNNERGTVACDDNVDNDNDGKTDFSLMAGVGDPGCSGPDDTNERGA
                     LACDDGVDNDGDGVSDYSTLAGGDPGCDSATDLSERGTAACDDGLDNDNDGNTDNLDF
                     GCMSPMDTSEHCMQGTGCPQCDDGVDNNADQKADFRLDGTGDPGCASPTDDEGGQSAC
                     NDGMDNDGDGATDAADPGCTDSSDTSERGTTTCDNGVDDDNDGLVDFRTDANGDPGCT
                     SVTDVSERGSLPCDNGSDDDDDGTQDFRLDGTGDPGCAAVTDPSERGTAICDDGIDND
                     NDGLVDFSMSAGGDPGCSGLTDGSEHGAMVCDDGVDNDSDGTIDFQLTNGDAGCANVA
                     DGSERGTIACDNGMDDDGDGAMDFVAGGGGDPGCADAVDTSERCGGTGCPACDNGLDD
                     DGDTLADAPADPQCAGPSDNNEQGASACSDGMDNDGDGLADANDPGCTSANDTSEKGT
                     TICDNGMDDDGDGTLDYVEMGGDPGCSSPTDGTETGTVACDNGMDDDNDGKTDYKTNG
                     TGDPGCAAPTDGSERGAGQCDDGADNDGDGRTDFNVTAALNDPGCSSPADASERGTLA
                     CDDGSDNDSDGRVDFNMNATLSDPGCTAPDDNNERGTLACDDGTDNDSDGFTDYSTNS
                     GTSDPGCTGPDDTSENCSGTGCPLCDDGMDNDNDQKTDMADCGCMTPADTQEAGVCTP
                     QCSDNIDNDNDGKTDMMDPGCANMMDNNEHAAGLTCDDGQDNDKDGLKDFQLSGGDPG
                     CTSLTDTNERGSLVCDDEIDADGDGEAAFATVGQFDTGCSSPFDTSELSTLQC"
BASE COUNT         3408 a         6389 c         6516 g         3152 t
ORIGIN      
        1 aagcccgaca cgaccaagcc cgacacgacc aaaccggata cgacgaagcc cgacacgacc
       61 aagcccgatg cgacgaagcc gaaccgctac gacctcgtcc agaaggtcga gcccggacga
      121 ccacgttcga agctggtgcc aatcctggtc ggcgtcggcg gtctcgcgct cgtcggcggt
      181 agcgtcgggt tcatggtgtg ggcgggctcg acgtacgaga aggcgcagaa cgaaggcaac
      241 gacgacaagc agctcgatct ctggcactcg gcgaacacca gacgctatgt cgccgaaggc
      301 cttggcgcgg ctggcatcgc ggcgatcggt gtttcggtgt ggctctacct tcgcagcggc
      361 agcgagccgc aacgtacgat cgatcatccg gcgatgatcg tgcctgccgt cgacggtgag
      421 cgcgcgacct tcgtcgtgag cggacgcttc tgatgcgcct tgcgtggata gcacccgcgg
      481 tcgtcctcgc gtccactgcg tgcggatttc ccaaacccgc ggatcgctgc gaagggccat
      541 gcagcgtgtt cgcgacggat cagccattcg cgtttgcggg tgacgaggtc tggctcgagg
      601 gcacgttcga agacggtgct ctcgtcaatt tcgccggcgc cagcgcaccg atcccgctca
      661 acgtgcgcta cgagcaccgg gcggcggtca cggttcccgc cgatgccacg acaggagcgc
      721 tctcgatcca gacgagcggc ggcatcgacg gcaaggttcc gttccgcggc ctgacgttca
      781 agcccgggct gcagccgttc atgccgcaca atgaccagaa cgactacgcg cggctcaact
      841 atctcgatcg cgtgcatgtc ggcggcactg ccgagacgat cggcaaccgt ctctacttga
      901 tcggcggcaa ggtcgacgtc gtcgtgacag ccgacgtcga gagtgcagcg atcggtctgg
      961 atggcggcat gggccgcccg aagatgttcg agtcgctgca gaccgcgcgc gcgtatcact
     1021 cgtcgattcg cgtcggtcgc tacatctatg tgatcggtgg tctcggttcg gggtcgctgc
     1081 gtagtgtcga acgcgcgcag atcggcgacg acgattcgct cgggccgttc gagacggtcc
     1141 caggtgtcga gctggtcgaa ggccgctacg gacacaagac gacgatcatc ggcaattatg
     1201 tctacgcgat tggcgggcga tcggtcagcg agctcgcgac cgtcgagcgc gcgccgatct
     1261 acgagaacga cgagctcggc acgttcgagc cgatctcgga tgtcgcgctg aagacaccgc
     1321 gttcgcactt cacgatcgtg aaaggcgcca cgtacctgta cgcgatcggc ggcttgcaca
     1381 acggcattcc gatcacgagc gtcgagaaga tcgagatgtt cggcaacggc tacgtctccg
     1441 acttccagga ggccggtgct ctgcgcgagc cgcgcggcaa ccacggcggg atcgtcgtcg
     1501 gcaataacat ttacgtgttt ggcggcgctg gtctcaccgg tccgctcgca agcgtcgaac
     1561 gtgcgccgtt cagcgaggat ggcacgctcg agttcttcgg cgagttcaat cacgtgctga
     1621 ccgaggccaa gcaggactca gccactgcgg tcatcggcaa ctacgtctac gtcatcggtg
     1681 gtgagcgcgg aggcaatgcg ccggcgctac tcgtcgagcg cgcaggtctc ttcgactacg
     1741 gtgcgctcga cacgttcgca aacgcgccgg tttcgctgaa ccagccgcgc tcgcaggccg
     1801 cggtcgttgt catcggcaag tggctctacg tgatcggtgg agtgaacggc acgggtgcgt
     1861 tgaatacgac cgaggtcgcg gagatcaaac ccgacggtag tctcagcgac tttggcatcg
     1921 tgcaagcacc cggtctgggc accgcgcgct acgggcatcg cgcgctgatc aatgacaacg
     1981 tgctgtacat cctgggcggc ctcgataccg caggcaatcc gctcaagaca atcgaggcag
     2041 cggccatcaa ccccgatggc tcgcttgcca actgggggta tcacggcacg cagatgttca
     2101 cggcgcgtgg ccggttctcg ctgttcacca gtggcatcac cgtctacggc ctcggcggct
     2161 acacgagcgc gacacaaccg acggcaacgg caacggtcga gtacgcatcg gtcaacgctg
     2221 gtacacagtt cggcagcttc aatgacctga gcgtcacgac gctcgacgat gcacgcgacg
     2281 gcgctgcgat ggtggcggtc ggccggtacc tgtacgttat cggtggcgac gcgtccgccg
     2341 gcagccaagt tgcacgcgct ctgacgaagc cgctcggcac cggctttacc ggtccgttca
     2401 cacccgctgg ggacccactc accaccgcgc gcagcggtca taccgtgacg cggctcggcc
     2461 attcgttcta cgtgatcggt ggtgccgata cgagcgtcac cgtcgaacga gcgatggtcg
     2521 atgacgtcga gagcaacgtc tttgggttct caccgtcgtc gaccgtgcag ctgatgacca
     2581 accggagcca ccacgcaacc gtgacgctcg gtaactacgt ctatctgatc ggcggcaagg
     2641 cggagccggc gaacggctcg tacttgtact cgctcgagcg cgcagcgatt cgctagcgat
     2701 catgaaacgc gttgtcgcgc tgctcgtcgc gttcggtctt gtcgccgcac tgatcgcgtg
     2761 gcggatgtgg ccccacggcg agacccacgc tgcagcgccg tcgtcaccga gcagcgcgac
     2821 gcagccaggc gcgacgagta ccgcgcagtc ctcgcgcgtg cgcaagctga gccccgagca
     2881 acggcgcgag ctcggcgaca agatcgccgc cgcaatcgaa cgcgcgcgca cggcgcaagc
     2941 ggcgagcacg tcgcacggta cgagcgcggg tagcgcaccg gcgctgcccg agccgccgtc
     3001 gatcccgctc gaagaagcgg cgaagccact gcaggaagcg ctccaggacg cgattccgct
     3061 gctcgccgac tgcttcgagc agcacgcgga taatcgtgct gctgcgatga tgacgatgac
     3121 gagcgaccca gacctcggca cggtgatcga taccgcagcg atcaccgaca aggatggcaa
     3181 gccgctcgag ccgaaggtcg acgagtgttt gcgcaatgca atcgattcgc tcgcgttgcc
     3241 gccgctcggg gcgcaggccg gcctgctgaa ggtgcaatac acatttcggt ttgacgagta
     3301 agatgagccg gtgcggctcg cgctattcgt gctcctgatc ctcgcggcac ccgtgcacgc
     3361 ggagccgacg aaggtgaagt caacggcact gacgctcggc aaggtgaagg caaagccggc
     3421 gcggctgctc gcaaaccgag tcacggtcga tgtcacgcgc tcggtgacgc cgtgcatctt
     3481 gaagctcgtc gtcgagccac aacatccgct gttcaagcgg ttcgcgatcg agctgggttt
     3541 cgaggtgaac gcgaccggtg cgatcaagaa cggcagtgga ccgctcaccg ccgaaatcaa
     3601 ggtcgctggc gcgagcgctc cgttcgtcgc aaagaccggc cgcgtcgacg tcacgcgcaa
     3661 gaagtcacat gacttcgacg ccaagctcat gctcgagttc gagcacgagg ccgtgccgtg
     3721 gacgctcagc ggcacggtgc gcatcgagaa cgccgcgtgc ctgtgggacg tcgagtagct
     3781 ataacgggcg aaccgacagg caccatccga ccgaggcgtt ttcacatagc agtcgtgtga
     3841 aaagtccccg cgtgccggcg tgaccgggtg ttcacgagac gggcccggaa ggctcggcga
     3901 tacgcctaca tggcgctggc atgcaggttg gacttggtcc gggcaaccgt caccaaaggt
     3961 ctaatgcgca ccacctctag cttgctgttt gtcgcccttc tctccgcttc gtcgtccgcg
     4021 ttcgctcaac ccgccgttcg cgatcatcgc gatccagctc cggttgctgc tgcggcgccc
     4081 gcatccgccc ccgaggttcg tgatcatcgc gataactcga tcgagggtcg tccgcgctac
     4141 cgccggcgtc cgggcccgcg cttcatgatg ccgctcaaga tcgacatcgg cgccgccggt
     4201 gcgaacacgt cgcgtggctt cgcgcccggc atgagtgctg cggttggtat ccactgggcg
     4261 tcgctgagcc cgcggccgac tgacaccgac gttggcatcg gcctgttcgg cggcatcctc
     4321 gccgcgcccg aggacacgag cgtgatgaac gacaagaacg gtgtcgcgta cggcggcgcg
     4381 tacctcgagc tcgcgcacac gctgtcgcaa ggtacctggt ggcgcacgtg ggcgtcgggc
     4441 cgtggcgagt atctcgggag caccgcgttc gaccaggacc acgctggctt tggcgcgtcg
     4501 ggtcggctca gcgcggagct ctacgtcagc ggtgtcggca tcgagccgcg cggcgtgttc
     4561 ctcggcacgt atgcgatcgg cgtctttgtc gaagcgggcg tgcgcgacat ggtcgaagac
     4621 gtcggcaagt tccagatgag cggcggcctc acgttccgca cgccgctcgt gttcgcgccc
     4681 taagcgtcag ccctacacgg gctgatccga cggcatgtcg cgggcttgcg ctcgcgatgt
     4741 cacaggcgcg ggagctacct cgtctcgagg gcatgttgaa ctggtttctc cgcaaacggc
     4801 tcacggcgtt cgagaaggac ttcggctatg acgtcagcta catgcgtcac gtgctcgacg
     4861 tggacatgcg ggcatttctc cgattcgccc gcgccgccag cgccgtgaag tatcgcaagg
     4921 acgtgccgat ggacctctat catgcggtcg gcttcaccgg catcgtcgcc gcggactgcg
     4981 gtccgtgcac gcaactcggc gtcacgatgg cgctgcgcga aggactgccg gcggcgacga
     5041 tcgccaagat cctccgcggc aacctcgacg acatgaccga accgacacag ctcggcgttc
     5101 gcttcgcacg cgcggtgatc gcgcgctcac ccgaagccga cgagctccgc gaagagatcg
     5161 tacgccgcca tggcccgcgt gctgtgatca gcctcggctt cgcgctcgtc gcgtcgcaga
     5221 tgtatccgat gttcaagtac gcgctcggct acggacatgc gtgtcagcgc atcgaagtgg
     5281 ctggcacgac cgtgaccccc aagctggcag tcgcatgagt gacttcgaag agcatcgtca
     5341 gttcttgatc ggtgtcgcgt accggatgct cggttcgttc gccgaggccg aggacatcgt
     5401 gcaggacgcg ttcttgcggt ggtcgaacac cgatcaagct gcggtcgagc acccgcgcgc
     5461 gtatctcgct cgcgtcgtgt cgcggctgtg cctcgatcgg atgaagtccg ccgcggcgcg
     5521 ccgcgagcag tacgtcggca cgtggctgcc ggaaccaatg gtcgccgagc cgaacgaacc
     5581 gttcgccgac gacctatcgc tcgcactact gctcacgctc gaacgtctct cgccgctcga
     5641 acgcgcggca tttctgcttc acgacgtgtt cgatatggac tacagcgcga tcgccgacac
     5701 gctggaacgc agcgaagccg cagtccgcca gctcgcgacg cgagcacgcg agcacgtgcg
     5761 tgaagagcgt ccgcgcttcg cggcgaccga cgagacccgt gcgcggctca ccgatgcgtt
     5821 ccagcacgcg attgccgacg gcgacatcgc gacgttggcg cgcctgctcg ccgacgatgc
     5881 agtgttctat tcagacggtg gtggcaagcg cctcgccgcg ctcaacccga tctacggcaa
     5941 agaaaagatc gtgcgattct tcatcggcat ctcgagcaag cgccctgtga tcgagcgcgt
     6001 cgaccacgtg cagatcaacg ggctgcctgg gttcgtcttc cacaccgacg agggcgtcga
     6061 gacgctcgcg ttcgatatcg cgggtgagca ggtcgtcgcg atctacgcga tccgcaatcc
     6121 cgacaagctg cggcacctgg cctagcgtcg cgggagcgca tgcaccgcgg cggtggcgcg
     6181 cgtgctcagg atggccgtgt cgattgccga caccgcatcg acgtgcttgc ggacatcgct
     6241 gaggcggcca tcggccgctg cgatctgcgc ggctacgcaa tgcgcgagcg cgatcgtgtt
     6301 ctcgtgttgc gaacggagcg ctgacgcgac tgcttcgtcg ctacgtcgcg ctgcttgcag
     6361 caagtcgccg cgcacgagct cccgctcggc gatcacgacg agagcaagcg ccagcaagtt
     6421 cggtgcatcg atcgcacgga gggcctcgat cgccggctcg agctccacgg gctcgtcgag
     6481 atcctggcgt gcgatcgctt cgagcaccgc gccgaacggt atgtcgcctg cctctccgat
     6541 gcgcaccgca tttgcccgga gcgccgcgca ataggggagg gcgtccaccg gacgcccgcg
     6601 gcgcacttcg atcaacgcgg cgcgggccca gccgagcgca cagagccacc acatgccagc
     6661 gcgctcggcg agttcgaagc cgcgacgcaa cagcgtgatc gccgcgtcgt catcaccttg
     6721 atgcatgcga atgaacgcga acgcgaacgg cagcgtcggg atgtcgaccg gcttctggct
     6781 gagcagcgcg gccgcctcat ttgccaactg ttcggcctcc gcgatctcgc gaccgaccag
     6841 cgcgaggcac tgcgccgagt acgcgagcgt gcgcgcacgg tcgtcgggtg cggcattacg
     6901 tgcgcgctcg gtggcttcga gcgacatcga tcgcgcacgg ccgtagtcac cggagccgaa
     6961 cggtatgtgc gcgagctcgt tgaggccacg cgcggcttca gcggcacaac cggcgatctg
     7021 ggcgtcgacg atcgcgcgct ctagtgcctt cgcgattgct gggcgacgct ggcggcggac
     7081 gtctgcgtgc agcgcagtga cgagcaggcc gatctgtagc cgcacccgct cgtgaccgac
     7141 gaggcgaacc gcgtgaccga gcccgcgctc ggcgagcgct gccgcttcct cgggcgcgaa
     7201 tagccgcatc gcgcgctcgg cggcggcgag acacgcggtc gcacacagtg ctgcgtcgtc
     7261 gccgagtccc gcatgatgcg cgatctggcc cgatcgatcg ccgtcgggat cgggcagcgc
     7321 tgcgagcgcg cgcgcgatct gtaagtggag cagccggcgg cgcggctccg agacctggtg
     7381 atacgcggcc gcacgcacga ggtcatgcgc gaagtcccac tcggcggcgt tcgcgctgag
     7441 gatggcgcgg cgttcgagct cttcgaacgc acgtgcgagc tcgacgagcg gcaaaccggt
     7501 gatcgtgccg agcacatcgg ccgagaatgc ggtgccgagc gcggcggccc acgggaggag
     7561 ctcgcgcgca gtgccctcga cgagctcgag gcgttcgacg agcagtgatt cgagcgacgc
     7621 ccacgtcgtg tcgccgcgcg cgagtgcacg cgcgatctcg acggcaaaca gcggatgtcc
     7681 gccgctctcg gcgaacacgc gcgcggcatc gacacccggc gcgtgggcga gcgcgagtgc
     7741 ggcggtctcg ctcgcgtcga gcggcgacag gccgatctgt tgcacggcgc cttcgcgggt
     7801 gaggccgcgc acgaggcgaa gcgcagatgc gttatcggcg agctcgccgg cgcgtgccgc
     7861 gagtgcgatc cggatctgcg tcgtgcgtgg cgaacgcgcg acatagtgga tcagcgcagc
     7921 ggtcgcttcg tcgatccact ggacgtcatc gaacacgagt acgagcgtgc cgccgtgcgt
     7981 gcggtcgacg agccattcga ccaccgactc gaacaggcgc gcgcgatcgg tctccgagac
     8041 gtttgccgcc ttgaatggat ggttggcgac actggccgtc tcgaacgcgt cgagccacgc
     8101 gccgtacggc cgcacttgtt ccgcttcgac gccgcggccg cgcaacagct cgatcgaacg
     8161 cgcatgcgcg agcgtgtcga gctcgccaag caagcgactc ttgcccatgc ccggatcacc
     8221 gatgagcaga acgacgcgcg tgcgcggttg atcgagcaga gccgcgagcg tcgcgcattc
     8281 cgacgctcgg ccgacgagcg cgacccgcgg ttgtccgcgc gcctccgcga tcagcgccgg
     8341 cacaggcgtg gcaacaggtg caggcacagg cgctgtttga ctgccaatca cgatgcgcag
     8401 ccgctcgagc tcgcgcgacg ggccgcggcc gagctcgcgc tcgagaagcc gcgcgcagct
     8461 cgcatattgc gcgagcgctt cggtcttgcg atcgagctcg acgagcacgc ggatcacggc
     8521 ggcatgggcg gcctcgtcga gcggatcgag tgcgagccac gttcgcgcgt gatcgagcgc
     8581 gtcggccggc gtgtcacgga ggcgttcgac aagcgccgac aacacggtca cgccgagccg
     8641 gcgagccgct tcgcgttcgg cgcgcagcca ttcgtcgtaa cgaaagcagt cggggaggtc
     8701 gagaccttcg agtagctcgc cgcgcacgag cggtgcgacc gcgacgagtg cctcggtcga
     8761 agcgcgcgca acgcccgcgg atgtgccgcc gggaattgcc gcacgcaacg tcacgagatc
     8821 gatcgcagca tcgccgggca cgagctcgac gcgatcgcgg tcggcgacca agcgcggcgt
     8881 gccgtcgtcg tcgacgagcg gacgcagctt cgtcaggctc cagcgtagcg cggcgcgtgg
     8941 atcatcggga ccgtcccaca gcagcgtgca caatcgctcg cgcagctggg gcttcccagt
     9001 cgccgcgaga tacgcgagca gcgcgcgcgt cttcttcgac gccggcaacg tgagcggctg
     9061 gccatcacgg gccacgacga gctcgccgaa gagccggatc tcgagtccgg ccttcgtgtc
     9121 agtcgctcgc cgtcgttccc gcacgtggct accctattac gggcgcgctt cgagcacgag
     9181 gttgaacggc gtctccgtcg cgcgccggaa tgccttccag ccgccgcggg tcgcgacatc
     9241 gcgcagccgt gcttcgcccg cctgtgcacc gagcgccggt ccgccctgcg acagcgagtg
     9301 aggaacgcac agcatcgtcg atgccgagta gaacacgcgt ccaacgggat tgtgattctg
     9361 ctcgggacgg tcgccggcga acggctctac gatcaaccac gtaccatctg gtgcgatcgc
     9421 ctcgcgcgcg cgcttcgcgg cgccgtacgg atcctcgagg tcgtgcaagc aatcgaagtg
     9481 cgcgatcaga tcgtagtcgc gacccgggaa gtcggtggcg cgcgcaacct cgaacgtcac
     9541 gcggtcggcg actccggcct cgcgtgcgcg ctggcgtgcg agctcgatcg acgcggcgtg
     9601 atagtcgaag cccacgaacg tcgatttcgg gtacgccttc gccatgatga tcgtcgatgc
     9661 gccaacgcca cagccgacgt cggcgacctt cgcgccgcgc tgcagtttcg cgacgacgcc
     9721 gtcgagcgcg ggcagccacg cctggatcag attgccgagg taccccgagc ggaagaaccg
     9781 ttcggtgccc tcgaacaagc acggatgctg atggccccac tcgaggcccg cgccggtgcg
     9841 gaagttcgcc gcgatcttcg actcgccggc ccacatcgcc gcggtcacct ggaacaaccc
     9901 gggcacgaat gccgggctct ccgaatcggc gagcgcgacc gcctgctccg gttccagctg
     9961 gtacgtgagc gtgtccttgg agtacgtgac gtagccaccg gccgccatcg cgttcaacca
    10021 ttcctgaatg tagcgctcgc tggtttcggt gcgcttcgcg agctcgctcg gtgacagcgg
    10081 cgcttcagcc cgcgcgagcg ctttccacaa tccgagcttg tcaccgatca cgacgagggc
    10141 cgcgctcatc gcgccgccga cgtccgtcac gaccttgccc aaaaactcgt tcaattttcc
    10201 gtcatcaacg ttcttcgtgg tcattggttc ctcctgtgag cacaccttcg accgcgggcg
    10261 tttgagccac cgtttgaatc tccgtggtat gaggcggcat gccgatcttc gtcgtcgaac
    10321 gtgtgttctc tccgccgatc actgaagcgc agttctacgc agcgggcgaa gtgctcgaga
    10381 aatgcatcaa ggagcgcgac atcaagtggc tctccagcca cttctccgcc gacggcagcc
    10441 gcagcatctg catgtatgac gcggccgacg ccgagcgtgt gcgcgaagcg aaccggactg
    10501 ccggtgcgcc gttcgagcgc gtgtggcaag cgcgcagcat gggctagccg aaactgcgaa
    10561 cgggagggca ctgccctccc gaccacgtgc gctcttgcga gtggttactt cttcgcagca
    10621 gccttcttca cgttggcaac gacgtgatcg acgtccttgc ggaactgact ctcgtcggcc
    10681 tgcgagccgt tgagcaccgt gctccagttg cgcgtgatcg cgccgatcgc gacggcctca
    10741 cgaatctccg cgtcggtcgc gccgttcagc ttcgcagcct cggtgtggaa gtagatgcag
    10801 tactgacacg ggatctgcgc cgcgaccgcg agaccgagca gctccttcgt cttgccgggc
    10861 agcgccgtcg acgggttgag ctggacggtc ttcatcatgc gccacgccga cgcgatgccg
    10921 acctccggga accggcggag gaacgtcggc accgaaccga gcgtgcgctc gatgtccttg
    10981 tatgccgacg ccgcgtcggt cactgccacc gcatcgccct tcggcgtcgg gttcttgacg
    11041 tacgcgaaca cgcgtccgac ctcttgcttg aactcggtga agtcgatctg caggccgttg
    11101 gccacggtgc tccagtggcg ggtcgcgcca gccatcgcga tcgcctccga gatttcgcgg
    11161 tcggtcgcgc cgttgagctt ggcgatctgg gtgtggaagt agatgcagta cttgcaagga
    11221 atctgtgcgg cgacgccgag cccgatcagc tcctttgtct tgccgggcag cgcggtcttc
    11281 gggttgagct ggacgctctt gagctcgtcc cacgccgcgg cgattccctc ctctggaaac
    11341 gccttgaaga actgcgggac aaagccgagc gtcttctcga tgtccttgta cgtggacttg
    11401 gcgtcgggtt cggcggcagc cgtcgagacg tggaacagcg cgatggtggc agcgatgatt
    11461 gcgagggtct tcatggtgtt tctctcctgt gagctcaccc tgaagccgca gcgttggaac
    11521 ccgcgttgga acgggcgttg aatccgttat catcggcccg tgaggtcgct ggcgacgatg
    11581 gtcggcctgg ggctggtagc ggcctgcggc gacaatttac gccctgctga caacgacata
    11641 cgcggtccgt acgtattgct cgatttcacc cggccgaccc tgttttcggc gcctgtgccc
    11701 gcgtgggatc tgacgaccga ggagcttgcg ggcatcgcca atccactgga ctcggcgatg
    11761 gtcgagcagg cgcgcgagct gctcgcgaac acgcgtgggt tctcgcgcgc aggtggcgtg
    11821 ttcttccaga tttcagaacc gctcgccgtc gacgagctgc cggggatcgc agagaccgtc
    11881 gccgcggatt cgccggtcat gctcgtcgat gtcgatccgt tgtcgcccga gctcggtcgg
    11941 cggattccac tcgaggtgag ctttcgcacc gacgcatcgg gctacggcgc gccgaatctg
    12001 ctcgcgctcg tgccgctgca gggtgtgccg ctgcaccggc gcactcgtta tgtcgccatc
    12061 gtgacgaccg cgctccacgc ggcgagcgga cccgcgctgt tgccagctcc tgcatcggta
    12121 ctggacaact tccgcgccgt gatcgcggcc gctggcgtgc cggagaccgc ggtcgctggc
    12181 gtcaccgcgt tcgacaccgg cgatccgacg gcggggctcg cggcagcgcg cactgccgca
    12241 ctcgctctgc cgctgccgac gctcgcgagc gcaccaacgc aaaccgatct gttcagcgac
    12301 ttctgcgtgt tccaatcgac gatcggaatg cccgactggc agagcggcgc accaccgttc
    12361 gcgacggacg gcggcacgtg ggagctcgac gcgaatggcg cgccgatcgt gcagcgcaca
    12421 gaggtcgcga aggtggtgat cacgctgccg cgcacggcaa agccaacggc cggctatccg
    12481 ctcgtcgtgt tcgtgcgcac gggcgccggc ggtgatcggc cgctcgtcga tcgcggtcag
    12541 cagtcgatgg acggtggccc gccgatcgtc gtcggtgaag gaccggcacg ctacctggcg
    12601 cgcgcgggct tcgcggcgat ccaggtcgat ggtccgctcg gcgggctgcg caacccgaca
    12661 agcggcgacg agcaattcct cacgctcaac ctcgagaacc tcggcgcgct gcgcgacaac
    12721 ctgcgcgagg cagcggtcga gctcgacgtg atcgcgcacg tggcgacgac gctgcatgtg
    12781 agcggtacga gctgcccggg ggccgccagc gacgccacgt ttgatgcagc gcacttcgcg
    12841 atcatgggac acggcatggg cgcgtggatc gcgcagctcg ccgctgcata cgagccactg
    12901 ttcggcgcgc agatcctgtc gggtgcgggc gcgagctgga tcgcgacatt gctcaatcag
    12961 cagaagccgc tcgcgctgcg gccgctcgtc gcgtcgctgc tgcgcgagca gacgatcgac
    13021 agcgcggatc cgattctgac gatcgtacag tgggcactcg agccggccga tcctcaagtg
    13081 tacgcctcga gcgcgcggcc tgtcttgcaa attgaaggcg tcgtcgacag cttcgcgccg
    13141 ccgcggatgg ccaacgcgtc tagcctcgcg ctgcgtctcg atctcgccgg ttcgtcgctc
    13201 gacgatgcag cggatccgcg ccttgtgggt cagctgccgc tcgtgccctt gcttgcactt
    13261 gtcggccgct cgcaggtcgc actgccagcg acaggatccg tcatcgcaca gttcgcggag
    13321 gacggcgtcg aggacgggca cgaggtgctc tatcaagtcg attctcccaa acatgggtat
    13381 cggtgctttc tcgcaagctg gctgaccgga accccgacgc tacctgtcgc tgcgtcgcgc
    13441 gacgcgccgt gtccataacg cgcaattcgc gatcggtccc gggcatccga tcgggtgagg
    13501 cgcgaaacgc gccagcaccg acacaagtgg tgccgataat cttcccttaa gatcacttgc
    13561 gggtttttac gtagcctcgc aaactggacc gtggtggacc gggctgcgcg tctgagcgct
    13621 gtccgtggtg ggtcatggcc gatctcatta caacgccaaa acatttgcgt tgcgcgagtt
    13681 gcggggcgaa gacaacgctc gagcagctga cgtgccccga gtgtggtgca ccgcagcggc
    13741 gcagcggcgt gcgaagcgcg ggatcggttc tcggctcgta tcgtttgatc gaagtgctcg
    13801 gcgaaggcgg catgggtgtc gtgtatctcg ccgagcacac gcgtctcggc cgccgcgtcg
    13861 cgctcaagac gctgcgcacg gacttcgccg agaacccgat ggcggtgcgt cgcttcttcg
    13921 gtgaagcacg cgccgtgaac aagatccgcc accagaacat cgtcgagatc accgacttcg
    13981 tcgaggaggc gggcggcgac aactactaca tcatggagct gttgcgcgga aagccgttgt
    14041 cgaacgtcat cgacaacgag ggcgtgcagc cgctcgcgcg cacggtcaac atcatggcgc
    14101 aggtcgcgcg cgcactgcag gcagtgcacg acgccggcat cattcatcgc gatctcaaac
    14161 ccgacaacgt gttcttgatc gagcgcggtg gccagcaaga cttcgtcaag ctgctcgatt
    14221 tcggcgtcgc caagctgatc gatgccgtcg attcgatcgg cgtgtcgtcg acggcagcag
    14281 gcgcgatcct cggcacgccc gaatacatgt cgcccgagca agcgagcggc aagaccgtcg
    14341 atcaccgcac cgacatctat tcgttcggca tcatgctgta cgagctcgtc accggtcagc
    14401 tgccactgcg cggtgacaac ttcggcgagc tggtcgtcaa gcacctgacg atcacaccga
    14461 cggcgccgag tcgtgtaccg gaccttccgc acgaggttcc ggcggagcta gaagacgcga
    14521 ttctcgcgtg catggagaag aacgaggacc tgcgaccgga tcggatcgac atcgtcggtg
    14581 atcagctcga agcgatcgca gccgccgccg gctgggagct gccgacgtat acggcggttc
    14641 cgcgcgcgac gggccggatg accgtgatga gcgcgcagac ggggagcgga ccggtgagtg
    14701 gcccggtgag catcgctccg ctacggcgca cgcctccgaa aggcgtaccg gcgctcgggc
    14761 acgcgccgac cctcgagatc gcaccgacgg gcagtggcga gatgcctgca ttgcgggcgc
    14821 cgtcgggaga gctgcggtcg ccgtccggcg agctgccgtc tgccgcatcg tcggtcgtgc
    14881 tccccacggc gccaccggcg aagcgctggc cgttgtacgc cgctgcggcc gccgtcgcgg
    14941 tgtcagcgat cgcgttcttg atcgtcaacc tgcgcggaaa cgacaagcct gctccacagg
    15001 ccgcaccacc cgtcgctgcg cccacgaaac cgaccgcgcc ggtcaacgag acgatcgaga
    15061 tcgagatcgt gaccacaccg gcaagtgcgg aggtcgtgcg ggcgtccgat ggtcacgtgc
    15121 tcggaaagac gccgctcgtg ctgccgttcg cgcgcgcgga tcgtgcggag atggtcgaga
    15181 ttcgcttggc cggatacgtc gcccagaagc agtcgttgcg actcgatcgc ggctcacgga
    15241 tttcgaccgt gctcgtgcct gttcccgcag agactgtcgc gaagccctcg cgctcgcgcg
    15301 gtaagccgac caagaaggcg caaccaacag cgacaggcac gccgacaaga cctgcggagc
    15361 cggcgaaggg ctcggcgaac gtcgaccgtt ccggtgtgat ggacccgttc aagtgaggat
    15421 ctgatgaccg ttctcgttcg cgctctcgcg atcgtttgca tgctgactgc cgtcggggca
    15481 gcgcagcctg caccgccacc ggcagatccg aaggtcgaag cgaagcggct gttcgacgaa
    15541 gcgaccgagc attacaagct cgccgagttc acccaggcgc tcgccggcta cgagaaggcc
    15601 tacgcactgt tcaaggcgcc ggcattcctg ttcaacatcg cgcagtgtca cttccagctt
    15661 cagacctggg accgagcgat cttcttcttc gagagctacc tgcgcgaaca gcctgacgcg
    15721 tcgaaccgca ccctcgtcga ggacctgatg cgcgaagcgc gcgaacgtca agccgccgcg
    15781 agacaagccg aacaacgcaa gctcgacctc gaaaaggaga ggctcgagct cgagaagcgg
    15841 gaaagcgaac acctcgccaa ggagctcgag ctacaaaccg catccttggt ggctcccacg
    15901 gagcctccgg tttacaagaa atggtggttc tggtcgatcg tcggcggcgt cgcggtcgcg
    15961 accattgcaa ccacggtcgc ggtcacgacg cgtgacaccg tgctgccatc gggcagcctt
    16021 ggaacggtgg atcaacgctg atgcgtacgc tcgcgctact caccgccgtc gggctcgggt
    16081 tggcgggctg cgctgacaag gtgcagaccg gcatcaccgg cgtcgagatc accgttacct
    16141 atccaaccga tcgcaacatc gatcagctct atgcgtgggg tatcgcggac ggcgagactc
    16201 cgctcgagcc gacgatcgtg ctcgagcatc cgcgcaagct gacctcgggt cgtgacagct
    16261 ttgtcgtcct cgtcgacaac agtgtcgccg gcagggagat gatcgtccgc gtcgacgggc
    16321 tagccggcag caccgtcgtc gcatcgggtc tcgaagtggt cacactcacc ggaggtaagc
    16381 tcacacacat caccgtcatg ctcggtgctg cggcggtgtg cggcgatggt gtgatcgcag
    16441 ccggcgtcga agggtgtgac gacggcgacc aggacgacgg cgatggttgt gcggcactgt
    16501 gtttcgtcga gtcgggctat tcgtgcaact ccgagccgtc gcgatgcacg aatggcatcg
    16561 cgtgcaacga cggtgtcgat aacgacggcg atggcctgat cgatacgtct gatccaggtt
    16621 gcgccgacac gatggatacc agcgagtccg gatcgaacgc gtgcgacgac ggaatcgaca
    16681 acgacaagga cggcaagatc gactaccgcg cggatagcac cggcgatgac ggctgcgctt
    16741 cgctgaccgg cacgaccgag ctcaacggtg gcgtatgtaa caacggcacc gatgacgatg
    16801 gcgatggctt gatcgactac cagactggct cgctcggcga tccgggctgc accagcgtga
    16861 tggacttgtc ggagcacggc gcggcgtcgt gtgacaacgg cgccgacgac gatggtgatg
    16921 gtgtcacgga ctatccgatg gacccgggct gcaccggcgc cgcagacact gacgagcacg
    16981 gctcgctcgc ctgcgacgac ggcaccgaca acgacagcga cggccgcacg gacttcctac
    17041 cgggcggtgc cggcgatccg gggtgcagca gtccgagtga caacaacgag cgcggcaccg
    17101 tcgcctgcga cgacaacgtc gacaacgaca acgacggcaa gacggacttc tcgctgatgg
    17161 ccggagtcgg cgatccggga tgctctggtc ccgacgacac gaatgaacgc ggcgcgctgg
    17221 cgtgcgacga cggcgtcgat aacgacggcg acggcgtctc cgactacagc acactcgcgg
    17281 gcggcgatcc cgggtgcgac tcggcgaccg atctgtcgga gcgcggcacg gcagcctgcg
    17341 acgacggtct cgacaacgac aacgacggca acacggacaa tctcgacttc ggctgcatgt
    17401 cgccgatgga cacgagcgag cactgcatgc agggcacggg ctgcccgcag tgcgacgacg
    17461 gcgtcgacaa caacgccgat cagaaggccg acttccggct cgacggtacg ggcgatccgg
    17521 gctgcgcgtc gccgaccgac gacgaaggtg gccaatcggc gtgcaacgac ggcatggaca
    17581 acgatggcga tggcgcgacc gatgcggccg atccgggctg taccgatagc tccgatacgt
    17641 ccgagcgcgg caccacgacg tgtgacaacg gcgtcgacga cgacaatgac ggccttgtcg
    17701 acttccgtac cgatgcgaat ggtgacccgg gctgcacgag cgtgaccgac gtgtcggagc
    17761 gcggctcgtt gccgtgcgac aacggcagcg acgacgacga cgacggtacg caggacttcc
    17821 ggctcgacgg caccggcgat ccaggctgtg cagcggtgac cgatccgagc gagcgcggta
    17881 ctgcgatctg cgacgacggc atcgataacg ataacgacgg cctcgtcgac ttctcgatga
    17941 gcgccggcgg cgatccggga tgctcgggcc tcacggacgg ctcggagcat ggcgcgatgg
    18001 tttgcgatga cggtgtcgac aacgacagcg acggcacgat cgacttccag ctcacgaacg
    18061 gcgacgccgg ttgtgcgaac gtggccgacg gctccgagcg cgggaccatc gcgtgcgaca
    18121 acggtatgga cgacgacggc gacggcgcga tggacttcgt cgccggtggc ggcggcgatc
    18181 cgggctgcgc ggatgcggtc gatacgagcg agcgctgcgg cggcaccggg tgccctgcgt
    18241 gcgacaacgg gctggacgac gatggcgaca cgctcgccga tgcgccggcc gatccacagt
    18301 gcgcgggccc gagcgacaac aacgagcagg gtgcgtcggc atgcagcgac ggcatggaca
    18361 acgacggcga cggtctcgct gacgcgaacg atccaggttg cacgtccgcg aacgacacga
    18421 gcgagaaggg cacgaccatc tgcgacaacg gcatggacga cgacggcgac ggcacgctcg
    18481 actacgtcga gatgggcggc gatccaggct gctcttcgcc gaccgacggc accgagaccg
    18541 gcaccgtcgc gtgcgacaac ggcatggacg acgacaacga cggcaagacc gactacaaga
    18601 cgaacggcac cggcgatccc ggttgcgctg cgccgacgga tggcagcgag cgcggtgctg
    18661 gccagtgcga cgacggcgcc gataacgatg gcgacgggcg caccgacttc aacgtgaccg
    18721 ctgcgctgaa cgacccgggc tgctcgagcc cggccgatgc gagcgaacgc ggcacgctcg
    18781 cgtgcgacga cggcagcgac aacgacagcg atggccgcgt cgacttcaac atgaacgcga
    18841 cgctctccga tccggggtgt actgcgccgg atgacaacaa cgagcgcggc acgctcgcct
    18901 gcgatgacgg cacggacaac gacagtgacg gcttcaccga ttactcgacg aatagcggta
    18961 cgtcggatcc tggatgcacg ggacccgatg acacgagcga gaactgctcg ggtaccgggt
    19021 gcccactatg cgacgacggc atggacaacg acaacgacca gaagacggac atggcggact
    19081 gcggctgcat gacgcccgcg gacacgcaag aagccggtgt gtgcacgccg cagtgcagcg
    19141 ataacatcga caacgacaac gacggcaaga ccgacatgat ggatccgggc tgcgcgaaca
    19201 tgatggacaa taacgagcac gcggctggcc tcacgtgcga cgacgggcaa gacaacgaca
    19261 aggatggcct caaggacttc cagctcagcg gcggtgatcc tggttgcacg tcactcaccg
    19321 acacgaacga gcgcgggtcg ctcgtgtgcg atgacgagat cgacgccgac ggcgacggcg
    19381 aggctgcgtt cgcgacggtc ggtcagttcg acaccgggtg ctcgagcccg ttcgacacca
    19441 gcgagctgag cacgctgcag tgtga
//
LOCUS       DASUFX010000137        11923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_439315,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000137 DASUFX010000000
VERSION     DASUFX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439315"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFV99_05445"
     CDS             <1..333
                     /locus_tag="VFV99_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058783.1"
                     /translation="GQTLALPASWSELAFGRGPWHGADHGAKNDLVVCTKTEFVSRRA
                     GRLYRAGHQLEVASLDQADELVVRRASGETVRPARTARGRAIVKPDDTIELGDGKGGA
                     VKLIVQRH"
     gene            335..1162
                     /locus_tag="VFV99_05450"
     CDS             335..1162
                     /locus_tag="VFV99_05450"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEX5058784.1"
                     /translation="MAAIHKDGIDQFRVKFAELARVRRGEPVHVDPFLIAQAIGLVMR
                     ECNVRSALGKPIVWNEYRVILARRDFDAIRPLQAPLERDLKTVLAQEAKSRQAELVGE
                     LRVTVVFDEADELSAAEGVVRVAFVPTEKLSAPKPGEMTMRLDSWAVSGEIAVKQASP
                     TDTVIVDDQTAPGHVVHWAGGSAQLPLGTTVVVGRPHPEAPAHFIGLAGAGAKVNKQH
                     CWLAATPTSLRIGRFAKANPVHVNGQPIAAGEDLEVALPAEVSLSRGDFVFTVQRRT"
     gene            1628..4537
                     /locus_tag="VFV99_05455"
     CDS             1628..4537
                     /locus_tag="VFV99_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5058785.1"
                     /translation="MGLELAAFGLAILVAWWLGSIGTTAWLARVAKLGLSAPSEPVVR
                     GSARIACLYFTVGIGALAAARVWCVRKAESAINVPWLLPVAVGAALFGLAVHLGTVEV
                     SKGAALLPTAGGFAQGFLVGCVLAAGILVAPVDIAELAARARKPIAITIAAIFAALAV
                     AGSGPAGSGTRINLGPLQPIELIKPLAILFLAVYLGSRASKLRWHRTRFLRLRWPRMD
                     LLVPAVGVLLLIVAGLYVIGDLGPVLLLALVFLGMFFVVSRATGWVVVALSILGGVLA
                     LLATWPGLAGSGTVKTRLVMWTDPWTNGLTNGSQLGEGLWAMSAGGWQGQGLGRALTP
                     LVPAGKTDLVLATLTEQMGALGLVAYQLALAALVIGALYVAARSRTAERVLIASGIAI
                     LFLAQWFVILGGTLGFLPLTGIVVPFVSAGRTSMAVFVALVALVARLATDGRVRVAST
                     ELDELHASTRGVRDLATLAIVFAIAASVNAAVGVRNTIATHTILARLRDGTLIARQNP
                     RLAVLAARVRRGEILDRNGIALATSPTPTTRKYPLGNALGTLMGVSPAKVLLPPWALE
                     RQFDAKLTGYADLAGFVPLLDLDDTAREKALAARDADIASRSVKLTLDSKLQAKVAAL
                     ARDAGKSWLTVAAVVMNAQTGEVLARVQVPDYDPNQPAWQDRVLANDTSFLARFRGAY
                     GEWPDKTGTQGMYQSGSVGKLFTAIAAVRANVPASQRFECREEDDQGPLFTLKGWPKP
                     IHDHTKDRPHGNPDLVEAIAVSCNVYFAQLGLLLGPHPFEELRKAGLEIGYGTKLVPG
                     DPGSRQLASTAFGQGVMAMNVMQAARMVAAIANGGRYIKCPPTMELGAKCTESQLVPD
                     PASLDVIRTGMSDVMTHGTGARLSPPAGIRVYGKTGTADVRGFAGEQPFGIAPRQIAA
                     PHSWFVAFAESAQGGTSSSRIVVAVVIPRGGTGASAAGPLAMQILAAARDLGYLP"
     gene            4581..6041
                     /locus_tag="VFV99_05460"
     CDS             4581..6041
                     /locus_tag="VFV99_05460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058786.1"
                     /translation="MPEDPDGTQHVEDGPAPATETQFVPDGTVLGPAVSKPNASSGAI
                     NPVRSGPAPRSPWARPMGSRPPDQTQHVDDLPRFGRFRALAEIGSGAMGTVYRAHDDV
                     LGRSVAIKALSSRDKSVRERFLREARAIGAVLHPNILAVYDAGTDDDTPYLVTELAAG
                     GTLRDRIHQGALSVDTVRELGIQIARALAAAHAANIIHRDVKPANILLAGDTWKLADF
                     GIARLPDSTLTVEGQFMGSPSYAAPESLRSGQFSPASDIYGLGATLYEALAGAPPHGD
                     HDMQSVVRKLEHDAPPLVTRRQVPGPLNDALMATLARDPAKRPTADELARLLANTSDE
                     QPVPRAPSTRTKVIALALAAIAIVLVIFAIVRDKPGSAPNLQPAFGPRGKVVTPPNEP
                     RRHDNPRARADEPRAHDDEQRGRNEDHFAEPPSAPEPPSAPEAPVEPSQPQPQVVDPW
                     GNPVDEETAAKILDQMHRDAQTEMDNWGHKRKRRKH"
     gene            6111..6719
                     /locus_tag="VFV99_05465"
     CDS             6111..6719
                     /locus_tag="VFV99_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058787.1"
                     /translation="MDRLACIVLCAVVSSGGAAYADDDYDAPAGPYRLLAGLEMGLTV
                     SPADGPRRDTLPIGGLAFRVRIPFSERTALSLGTSIPFPPFITGQVIALYEWGPIRST
                     SGLVLRGGIRPLVGLVGLCDASGEAQCPLDRAAEPGDRAGWAIGVMSELGAGWRFRLG
                     SKVSLEAIASYLGGLFDGRQRKTESALDGYYQGGMLSIDLLF"
     gene            complement(6763..9183)
                     /locus_tag="VFV99_05470"
     CDS             complement(6763..9183)
                     /locus_tag="VFV99_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5058788.1"
                     /translation="MGACRSLAIAVVVLCGCGDNIHPGGGTLMVSPQLDLHTTELGGT
                     ATFTVALTNQPLEKVTIEITSMDTTEGTVSPATLSFDRDNFADARTVTVTGVDDDRAD
                     GDQSYLVRVGSDELGAVDLDITNDDDDVAGFEVSPVVGLETSESGATAMFSVMLTSQP
                     AADVMVPVTSSDPTEGVTDRASLVFTDTNWFSPQTVTVTGQPDLISDGNVSYTIMLGA
                     ASSSDNGYEGLDPDDVTLMNVDENVGGISVSAPATLMTTEGGGQATFSVVLMTQPMAD
                     VTIPIASTDTGEASVSTASLVFTAANWNVAQTVTVTGVDDSIIDGPQPFTITLGPATS
                     TDTSYAGLDPNDLAGVNADNDSASITVQPTSGLVTSEAGTTATFGIVLGSQPAANVTI
                     AVMSSDASEASVSTTQVTFTSANWSTVQTVTVTGVDDVIDDGDQPFTILLAPAASGDP
                     AYSGIDPDDPTGTNTDNDTSAIVVTPTSGLVTSEAGGADSFTVVLTSQPSGNVTIPVA
                     SNDTTEGTVSPTSITFTPANWNVARTVTVTGVNDILADGTQTYTIVLSPATSSDTLYN
                     GINPADVTVQNLDNDIASVTVDPINGLVVSEFQDTDTFTIVLDTQPIANVTISLMSSD
                     TTEGTVLPASVTFTPANWNQPRTITVTGVDDTLNDGNQNFTIITGNAVSLDPRYSGIA
                     VANVDVTNIDNDIAQVYVKARRRLLVSENGQSATFRVRLTIPPTAQVTCTLQSTDLTE
                     GLVSPTTLNFTPGNFGFQTVTVSGVDDTMVDGDVTFTVQLNACTSTDNAYNGSNPRDV
                     TAVNRDND"
     gene            complement(9208..10026)
                     /locus_tag="VFV99_05475"
     CDS             complement(9208..10026)
                     /locus_tag="VFV99_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058789.1"
                     /translation="MSGPIGTLDAMAIDYLLGVGCQPQRELGVEKLVNLNRTRILARS
                     ALAHMREDGDARAPNEIEIQLTMRTTDGDSARGVTLQDLLEESRPLDSVAEYCGNCPA
                     HLPREFACHRRIRYPIPERVEAWLMSRLPESLACTAGALLVRGLAEFGWDGAPTAKMR
                     SAGTTFFESRAPYGVRWESDDGVIEISSDQLFQMMFMVGNLQPTHCLMLALFFGVIPH
                     DTSLHDLKDEPGRTRALEAANVATEADADIEQLAAFLRALAVAARLDVPILVDG"
     gene            10047..10120
                     /locus_tag="VFV99_05480"
     tRNA            10047..10120
                     /locus_tag="VFV99_05480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10081..10083,aa:Arg,seq:ccg)
     gene            10124..10837
                     /locus_tag="VFV99_05485"
     CDS             10124..10837
                     /locus_tag="VFV99_05485"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058790.1"
                     /translation="MKTLAEIAIEAGLVTKAGAAKAGRMAEERKEPLVVVLIRELGVN
                     EVALIGALRKQTRVPLLDPAQVEVDPEALRIVPRDSCARLRVLPLGLTLDGKTRVLRV
                     AMADPTDTSAILELEVIANCEIEVTALPLSAIDELVDKGYKQFSTAVMSRQKLFGRNL
                     TVTTKNNKIQPIDSAEAAGASEVSVTAQIQLASLVAAPDPEERLAALIHVLVSKGVVT
                     EVELADALKKLESGKPSES"
     gene            10946..>11923
                     /locus_tag="VFV99_05490"
     CDS             10946..>11923
                     /locus_tag="VFV99_05490"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058791.1"
                     /translation="MITCAGCKHSYADSALYCPNCGTPKAREAIATDPLVGKVLGERF
                     QVIELLGQGGSGTIYRAEHVTLRRKVAIKVLHNELSRDDLAVERFRREATTVAEIDNE
                     HIVEIHDFGRAHDGRLYLAMELLEGETLDGVLARERQLSVERTADILIQVGEALMEAH
                     AIGYVHRDLRPRNIYLANRRGKANFVKLLDFGLAKLVEGDAQAASTSLGMTFGDPRYM
                     SPEQARGDRIDRRADIYQLGCIAYEMLTGAPPFTGNRVFDILSKQVTEIPAPLPTRRP
                     GVPLWMEAAVAKMLAKDPDNRFATTTRLVEALRRGIDTGDVMEDEIARRR"
BASE COUNT         1887 a         3956 c         4038 g         2042 t
ORIGIN      
        1 ggccagacac tcgcgctccc ggcgagctgg agcgagctgg cgttcggtcg cgggccgtgg
       61 cacggagctg accacggtgc gaagaatgat ctcgtcgtct gcaccaagac cgagttcgtc
      121 agccgccgcg cgggacggct ctatcgcgcg ggacatcagc tcgaggtcgc gtcgctcgat
      181 caagcggacg agctcgtcgt gcggcgcgcg tcgggggaga ccgtgcggcc tgcccgcacc
      241 gcacgcggcc gtgcgatcgt caaacccgac gacacgatcg agctcggcga tggcaaaggc
      301 ggcgcggtga aactgatcgt gcagcgacac tgacatggcg gcgatccaca aagacggcat
      361 cgatcagttc cgcgtgaagt tcgcggagct cgcgcgcgtg cgtcgcggcg agccggtgca
      421 tgtcgatcca ttcttgatcg cgcaggcgat cggcctcgtg atgcgcgagt gcaacgtgcg
      481 ctcggcactc ggcaagccga tcgtatggaa cgagtatcgc gtgatcctcg cgcggcgcga
      541 cttcgacgcg atccggccac tgcaggcgcc gctcgagcgc gacttgaaga ccgtgctcgc
      601 gcaggaggcg aaaagccgcc aggccgagct cgtcggcgag ctgcgcgtca ccgtcgtgtt
      661 cgacgaagcc gacgaattgt ccgcggccga gggcgttgtg cgcgtcgcgt tcgtcccgac
      721 cgagaaactg tcggcgccca agcccggcga gatgacgatg cgactcgaca gctgggccgt
      781 ctcgggagaa atcgcagtca agcaagcgtc gccgaccgac acggtcatcg tcgacgacca
      841 gaccgcgccc ggccacgtcg tgcactgggc cggaggcagt gcgcaactgc cgctcggtac
      901 aaccgttgtc gtcggtcgtc ctcatcccga agcgcccgca catttcatcg gcctggccgg
      961 ggccggggcg aaagtaaaca agcagcattg ctggcttgcc gcgacgccga cgagcctgcg
     1021 catcggcagg ttcgccaagg caaatccggt ccacgtcaac ggtcaaccga tcgccgccgg
     1081 tgaggatcta gaggtggcgc tgccggccga ggtctcgctg tctcgtgggg acttcgtctt
     1141 taccgtgcag cgccgcactt gaagccgtgc cggcagagct cactctagga catgtgttgc
     1201 gccgtcttgc gtcgcgcgac cggagtcgcg tccggagatc taacgccaag acgagccaag
     1261 gtcgccaaga acgccaagaa gaagggttct tgatcgcgct ctgcacgcgc gatcattcga
     1321 gcagaacgcg atcggaaaac ctttccttgg cgttcttggc gttcttggca tcttggcgtt
     1381 ccctctggga ggaatcgaaa tggggtcaga ccccattttc tagaggctgc gtccacgcgc
     1441 cggacgactt tgcgaaatgg ggtcagaccc cttttctggg gtcgaccgcg ttctgcgcgc
     1501 gcgatcgttc gagcagaacg cgatcggaaa aaggtccctt ggcgttcttg gcgttcttgg
     1561 catcttggcg ttacatctgg gcggggagcc gatgacgcgc gggcgttggc ttgtggtcgg
     1621 tggttcgttg ggcctggagc tcgcggcgtt cgggctcgcg atcctcgtcg cgtggtggct
     1681 cggatcgatc ggcacgactg cttggctcgc acgggtcgcc aagctcggac tctccgcgcc
     1741 cagcgagccg gtcgttcgcg gcagtgcgcg gatcgcatgc ctctacttca cggtcggcat
     1801 cggcgcgctc gcggccgcgc gcgtgtggtg cgtgcgcaaa gcggagagcg cgatcaacgt
     1861 gccgtggttg ctgcccgttg cggtcggtgc cgcgctgttc ggattggcgg tgcatctcgg
     1921 gaccgtcgag gtctcgaagg gcgccgcgct gctgccgacc gcgggtggtt tcgcgcaggg
     1981 ttttctcgtc ggctgcgtgc tcgcggccgg gatcctcgtc gcgcccgtcg acattgcgga
     2041 gctcgcggcg cgtgcgcgca aaccgatcgc gatcacgatc gctgcgatct tcgcagcact
     2101 cgcggtcgct ggctcgggac cggcgggctc gggcacgcgg atcaatctcg ggccgctgca
     2161 accgatcgag ctgatcaagc cgctcgcgat cttgttcctc gccgtgtatc tcggctcgcg
     2221 cgcgagcaag ctgcgctggc accgcacgcg gttcttgcgg ctgcgctggc cgcgcatgga
     2281 tctgctcgtg ccagcggtcg gcgtgctgct gctgatcgtc gccggcctgt acgtgatcgg
     2341 cgacctcggt ccggtgctgc tgctcgcgct cgtgttcctc ggcatgttct tcgtcgtcag
     2401 ccgcgcgacc gggtgggttg tcgtcgcgct gtcgatcctc ggcggcgtgc tcgcgctgct
     2461 cgcgacgtgg cccgggctcg ccggcagtgg cacggtgaag acgcggctcg tgatgtggac
     2521 cgatccgtgg acgaacgggc tcacgaacgg ctcgcagctc ggcgagggcc tgtgggcgat
     2581 gagtgccggc ggctggcaag ggcagggcct cggccgcgcg ctgacgccgc tcgtgcccgc
     2641 cggcaagacc gacctcgtcc tcgcgacgct gaccgagcag atgggcgcgc tcggcctcgt
     2701 cgcgtatcag ctcgcactcg ccgcgctcgt gatcggcgcg ctctacgtcg ccgcgcgcag
     2761 ccgcaccgcc gagcgtgtgc tgatcgcgag cggcattgcg atcctgttcc tcgcgcagtg
     2821 gttcgtgatc ctcggcggca cgctcggttt tctgccgctg actggcatcg tcgtgccgtt
     2881 cgtgtcggcg gggcgcacga gcatggcggt gttcgtcgcg cttgtcgcac tcgtcgcgcg
     2941 gctggcgacg gacggccgcg tgcgcgtcgc atcgacggag ctcgacgagc tgcacgcgtc
     3001 gacgcgcggc gtgcgcgacc tcgcgacgct cgcgatcgtg tttgcgatcg cagcgagcgt
     3061 caatgcggcg gttggcgtgc gcaacacgat cgcgacgcac acgatcctcg cgcgcctgcg
     3121 cgacggcacg ttgatcgcgc ggcagaaccc gcggctcgcc gtgctcgcgg cgcgcgtgcg
     3181 acggggggag atcctggatc gcaatggcat cgcgctcgcg acgagcccca caccgaccac
     3241 gcgcaaatat ccgctcggca atgcgctcgg cacgctgatg ggcgtgtcgc cggcgaaggt
     3301 gctgctgccg ccgtgggcgc tcgagcgtca gttcgacgca aagctcaccg gctacgccga
     3361 cctcgcgggg ttcgtgccgc tgctcgatct cgacgacact gcgcgggaga aggcgctcgc
     3421 cgcgcgcgac gccgatatcg cgtcacggtc ggtcaagctc acgctcgaca gcaagctgca
     3481 ggcgaaggtc gccgcgctcg cgcgcgatgc cggcaagagc tggctcaccg ttgcggcggt
     3541 cgtcatgaac gcgcagaccg gcgaggtgct cgcgcgcgtg caggtgcccg actacgatcc
     3601 aaaccagccg gcgtggcagg accgcgtgct cgcgaacgac acgtcgtttc tcgcgcggtt
     3661 tcgcggcgcg tacggcgaat ggcccgacaa gaccggtaca cagggcatgt atcagtcggg
     3721 ttcggtcggc aagctgttca ccgcgatcgc cgcggtccgc gcgaacgtgc cggcatcgca
     3781 gcgcttcgag tgccgcgagg aggacgacca gggcccgctg ttcacgctca agggctggcc
     3841 gaaaccgatc cacgatcaca cgaaggatcg gccgcacggc aatcccgatc tcgtcgaggc
     3901 aatcgcggtc agctgcaacg tgtacttcgc ccagctcggc ctgttgctcg gtccacatcc
     3961 gttcgaggag ctacgcaagg ccggcctcga gattggctac ggcaccaagc tcgtaccggg
     4021 cgaccctggc tcgcgtcagc tcgcgtcgac ggcgttcggc cagggcgtga tggcgatgaa
     4081 cgtgatgcag gcagcgcgca tggtcgcagc gatcgcgaac ggcggccgct acatcaaatg
     4141 ccccccgacg atggagctcg gcgcgaagtg caccgagtcc cagctcgtcc ccgatccggc
     4201 gagcctcgac gtgatccgga ccggcatgag cgacgtgatg acgcatggca ccggcgcgcg
     4261 gctgtcgcca ccagccggca tccgcgtcta cggcaagacc ggcaccgccg acgtccgcgg
     4321 cttcgccggc gagcagccat tcggcatcgc cccacggcaa atcgcggcac cccactcgtg
     4381 gttcgtcgcg ttcgccgagt ccgcgcaggg cggaaccagt agctcacgca tcgtcgtcgc
     4441 ggtcgtgatt ccgcgcggcg gcaccggcgc atccgccgcc ggtccacttg cgatgcagat
     4501 cctcgcggcg gcgcgcgacc ttggctacct cccatgacag tgcttgcgcg cccaccggac
     4561 gtgacgtcat gatttcgtcg gtgcccgagg atcccgacgg aacgcagcac gtcgaagacg
     4621 gaccggctcc tgctacggag acgcagttcg tccccgacgg caccgtgctc ggtcccgcgg
     4681 tctcgaagcc gaacgcctcg tcgggtgcga tcaacccggt gaggtccggt cctgcgccgc
     4741 gctccccgtg ggcacggccg atgggctcgc ggccgccgga tcagacgcaa cacgtcgacg
     4801 acctgccgcg cttcggtcgg ttccgcgcgc tcgccgagat cggcagcggt gcgatgggca
     4861 cggtctatcg cgcgcacgac gatgtgctcg gtcgctccgt ggcgatcaag gcgttgtcat
     4921 cgcgcgacaa gagcgtgcgc gaacggttcc tgcgcgaggc acgcgcgatc ggcgctgtgc
     4981 tgcatccgaa catcctcgcg gtctacgacg cgggcaccga cgacgacacg ccgtatctcg
     5041 tcaccgagct cgccgcgggt ggcacgctgc gcgatcgcat ccaccaaggc gcgctctcgg
     5101 tcgacaccgt gcgcgagctt ggcatccaga tcgcccgcgc actcgccgcc gcgcacgctg
     5161 ccaacatcat ccatcgcgac gtcaagccgg cgaacattct cctcgcgggc gacacgtgga
     5221 agctcgccga tttcggcatc gcgcggctgc ccgactcgac gctcaccgtc gagggtcagt
     5281 tcatgggctc gccgtcgtac gcggcgcccg aatcgctgcg ctccggtcaa ttcagcccgg
     5341 cgtccgatat ctacggcctg ggcgccacgc tctatgaagc gctcgccggt gcgccaccgc
     5401 acggcgatca cgacatgcag tccgtcgtgc gcaagctcga gcacgacgcg ccgccgctgg
     5461 tcacgcgccg ccaggtgccc ggtccgctca acgacgcgct gatggcgacg ctcgcgcgcg
     5521 atccggcgaa gcggccgacc gccgacgagc tcgcgcgcct gctcgcaaac acgagcgacg
     5581 agcagccggt gccccgcgcg ccatcgacgc gtacaaaggt gatcgcgctc gcgctcgccg
     5641 ccatcgcgat cgtcctcgtg atcttcgcga tcgtgcgtga caagccaggc agcgccccga
     5701 acctgcagcc tgcgttcggt ccgcgcggga aggtcgtgac gccgccgaac gagccgcgca
     5761 ggcacgacaa tccgcgcgcg cgcgccgacg agccgcgcgc acacgacgac gagcagcgcg
     5821 ggcgcaacga ggatcacttc gcggagccgc cgagtgcacc cgagccaccg agtgcgccgg
     5881 aagcgccggt cgagccctca cagccgcagc cgcaggtcgt cgatccgtgg gggaacccgg
     5941 tcgacgagga gacagcggcg aagatcctcg accagatgca ccgcgatgcc cagaccgaga
     6001 tggacaactg gggccacaag cgcaaacgcc gcaagcatta aacgggctcc gtgctgccgc
     6061 cgcatcgatc gaagcaaccc ggtactgctc gatccgatat ggagcggatc atggaccggc
     6121 ttgcctgtat cgtgttgtgt gctgtcgtat cgagcggtgg cgcggcgtat gccgacgacg
     6181 actacgacgc gcccgctgga ccttaccgct tgcttgccgg tctcgagatg ggtctgacgg
     6241 tcagtcccgc cgacggacca cgccgagaca cgttgccgat cggcggcctc gcgtttcgcg
     6301 ttcgcatccc gttctcggag cgcaccgcgc tgtcgctcgg cacatcgatc ccgtttccac
     6361 cgttcatcac gggacaagtc atcgcgctct acgaatgggg accgattcgc tcgacctcgg
     6421 ggctcgtgtt gcgcggtggt atccgcccgc tcgtcgggct cgtggggctg tgcgacgcca
     6481 gcggagaagc ccagtgtccg ctcgatcggg ccgccgagcc tggcgatcgc gcgggctggg
     6541 cgatcggtgt gatgagcgag ctcggtgccg gttggcggtt tcggctcggc tcgaaggtgt
     6601 cactcgaagc gatcgcctcg tacctcggcg gtctgttcga cgggcggcag cgcaagacgg
     6661 agagcgcgct ggatggctac taccagggcg gcatgctgtc gatcgacctg ctgttctgaa
     6721 gtaacgatcg cgcgcgccga caagaatggt ggggcgcgtc ctctaatcgt tgtcgcggtt
     6781 gaccgcggtg acgtcgcgcg gattcgagcc gttgtaggca ttgtccgtgc tcgtacacgc
     6841 gttgagctgc acggtgaacg tcacgtcacc gtcgaccatc gtgtcgtcga cacccgacac
     6901 ggtgacggtc tggaagccga agttgcccgg cgtgaagttg agtgtcgtgg ggctgacgag
     6961 accttcggtt agatccgtgc tctgtagcgt gcaggtgacc tgcgctgttg gcgggatcgt
     7021 cagccgcacg cggaacgtcg cggactggcc gttctcggag acgagcaagc gcctgcgcgc
     7081 cttcacgtag acctgcgcga tgtcgttgtc gatgttcgtc acgtctacgt tggcaactgc
     7141 gatgccggag tagcgcgggt cgaggctgac cgcgttgccc gtgatgatcg tgaagttctg
     7201 gttgccgtcg ttcagcgtgt cgtcgacacc ggtcaccgtg atcgtgcgcg gttggttcca
     7261 gttcgcaggc gtgaacgtca cgctcgccgg cagcacggtg ccttcggtcg tgtcgctgct
     7321 catcagcgag atcgtcacgt tcgcgatcgg ctgggtatcg aggacgatcg tgaacgtgtc
     7381 ggtgtcctgg aactcggaga cgaccaaccc gttgatcgga tcgacggtga cgctcgcgat
     7441 gtcgttgtcg aggttctgta ccgtgacgtc ggccgggttg atgccgttgt agagcgtgtc
     7501 gctcgacgtc gcgggcgaca gtacgatcgt gtacgtctgc gtgccgtcgg cgaggatgtc
     7561 gttgacgccc gtcaccgtca cggtccgcgc gacgttccag tttgccggcg tgaacgtgat
     7621 cgacgtcggc gacactgtac cctcggtcgt gtcgttcgac gcgaccggaa tcgtgacgtt
     7681 gccgctcggc tgcgatgtca gcacgacggt gaacgagtcg gcgccgcccg cttcgctcgt
     7741 cacgaggccg ctcgtcggcg tcacgacgat cgcggacgta tcgttgtcgg tgttggtgcc
     7801 ggtcggatcg tctggatcga ttccgctgta cgcaggatca ccgctcgccg ccggcgcgag
     7861 caggatcgtg aatggttgat cgccatcgtc gatcacatcg tcaacgccgg tcaccgtcac
     7921 ggtctgcacc gtgctccagt tcgcggacgt gaacgtaacc tgcgtcgtcg acaccgaggc
     7981 ttcgctcgca tcgctcgaca tcaccgcaat cgtgacgttc gccgcgggct gcgagccgag
     8041 cacgattccg aacgtcgcgg tcgtgccggc ttcgctggtc acgaggccac ttgtcggctg
     8101 taccgtgatc gaggcgctgt cgttgtccgc attgacgccg gcgagatcat tcgggtcgag
     8161 acccgcgtag ctcgtatccg tgctcgtcgc tgggccgagc gtgatcgtga acggctgcgg
     8221 accgtcgatg atgctgtcgt cgacgccggt cacggtgacc gtctgcgcga cgttccagtt
     8281 cgcggccgtg aacacgagcg acgccgtcga caccgacgcc tcaccggtgt cggtcgacgc
     8341 gatcggaatc gtcacgtctg ccatcggctg cgtcatcaat acgaccgaga acgtcgcttg
     8401 cccgccacct tcggtggtca tcagcgtcgc cggcgcgctg accgagatgc cgccgacatt
     8461 ttcgtcgacg ttcataagcg tgacgtcgtc gggatcgagg ccttcatagc cgttgtccga
     8521 gctcgatgcc gcgcccagca tgatcgtgta gctgacgttg ccgtcggaga tcagatcggg
     8581 ctggccggtc acggtgaccg tctgcggcga gaaccagttc gtatcggtga acacgagcga
     8641 cgcgcggtcg gtgacgccct ccgtcggatc cgagctcgtg actggcacca tcacatcggc
     8701 cgccggttgt gacgtcagca tgaccgagaa catcgctgtc gcgccgctct cgctcgtctc
     8761 gaggccaacc actggcgata cctcgaagcc ggcgacatcg tcgtcgtcgt tcgtgatgtc
     8821 cagatcgact gcgccgagct cgtccgatcc gacgcgcacg aggtagctct gatcgccatc
     8881 ggcgcgatcg tcgtcgacgc cggtcaccgt caccgtgcgt gcatcagcga agttgtcgcg
     8941 atcgaacgac agcgtcgccg gcgacaccgt gccctcggtc gtgtccatgc tcgtgatctc
     9001 gatcgtgacc ttctcgagcg gctggttggt cagcgcgacc gtgaacgtcg cggtcccgcc
     9061 cagctccgtc gtatggagat cgagctgtgg cgacaccatg agcgtgccgc caccgggatg
     9121 gatgttgtcg ccgcagccgc acaagacgac gacggcgatc gcaagcgatc ggcaagctcc
     9181 catcgtggaa aatggtatca gggatcgtca gccgtcgacg agaatcggca catcgagccg
     9241 ggccgccacc gcgagtgccc gcaggaacgc ggcgagttgt tctatatcag catcggcttc
     9301 tgtcgcgacg ttagccgctt ctagggcacg tgtgcgcccg ggctcgtcct tgaggtcatg
     9361 caggctcgtg tcgtgcggaa tcacgccgaa gaacagcgcg agcatgaggc aatgggtcgg
     9421 ctgcaggttg ccgaccatga acatcatctg gaacagctga tccgacgaga tctcgatcac
     9481 accgtcgtcg ctctcccagc gcacaccgta gggcgcacgg ctctcgaaga atgtcgtacc
     9541 tgcagaacgc atctttgcgg tcggcgcgcc gtcccagccg aactcggcga gtccgcgcac
     9601 gagcaacgcg ccggcggtgc atgcgagcga ttccggcagg cgcgacatca gccacgcctc
     9661 gacgcgctcg ggaatcggat agcgaatgcg gcgatgacac gcgaactcgc gcggcaggtg
     9721 cgcggggcag ttgccgcaat attcggcgac gctgtcgagc ggccgcgact cttcgagcaa
     9781 gtcctgcagc gtgacgccgc gcgcggaatc gccgtcggtc gtgcgcatcg tcagctggat
     9841 ctcgatctcg ttcggcgcgc gcgcgtcgcc gtcttcgcgc atgtgagcga gcgcggagcg
     9901 ggcgaggatg cgtgtgcggt tcaggttgac cagcttctca acgcctagct cgcgttgtgg
     9961 ctggcacccg acgccgagga gataatcgat cgccattgcg tcgagcgtac caatggggcc
    10021 cgacataggg taagaacagc cccgacgcac ccgtagctca gctggataga gcgttggcct
    10081 ccggagccaa aggtcacagg ttcgaatcct gtcgggtgca cgagtgaaga cgctcgcgga
    10141 gatcgcgatc gaggccggcc tggtgacgaa agccggtgcc gcgaaggccg gccgtatggc
    10201 cgaggagcgc aaggaaccgc tggtcgtcgt gctcatcagg gagctcgggg tcaacgaggt
    10261 cgccctgatc ggcgcactgc gtaaacaaac gcgggtgccg ctgctcgacc ccgcgcaggt
    10321 cgaggtcgat cccgaggcgc ttcgtatcgt gccgcgcgat tcatgcgccc ggctccgggt
    10381 gctgccgctg gggctgaccc tcgacggtaa aacccgcgtt ctccgcgtcg cgatggccga
    10441 cccgaccgac acctccgcga tcctcgagct cgaggtcatc gcgaactgcg agatcgaagt
    10501 gaccgcgctc ccgctgtccg cgatcgacga gctcgtcgac aagggctaca agcagttctc
    10561 gacggccgtg atgagccggc agaagctgtt cggccgcaac ctcaccgtca cgacgaagaa
    10621 caacaagatc cagccgatcg acagcgcgga ggccgccggc gcgtcggaag tgtcggtgac
    10681 cgcacagatt cagctcgcgt cgttggtcgc ggcgccagac cccgaagagc ggctggctgc
    10741 gttgattcac gtgctcgtct ccaagggggt cgtgaccgag gtcgagctgg ccgacgccct
    10801 caagaagctc gaatccggca agccctcaga atcttgagag ttggccaggt tttgccacgg
    10861 gcatcccagg caaccctgcg ctaccatctc ggagtcgtcc gtcaggcgac acatgtcccg
    10921 tcatgagatg actaccgcgt cttcaatgat cacctgcgcc ggttgcaagc acagctatgc
    10981 cgattcggct ctgtattgcc cgaactgcgg cacgcccaag gctcgcgaag ccatcgcgac
    11041 agatccgctc gtcggcaagg tactcggcga gcgcttccag gtgatcgagc tgctcggcca
    11101 aggtggctcc ggcacgatct atcgcgccga gcacgtcacg ctgcgccgca aggtcgcgat
    11161 caaggtgctc cacaacgagc tctcgcgcga cgacctcgca gtcgagcggt ttcgccgcga
    11221 ggcgacgacg gtcgcggaga tcgacaacga gcacatcgtc gagatccacg acttcggacg
    11281 cgcgcacgac ggccggctct atctcgcgat ggagctgctc gaaggcgaga cgctcgatgg
    11341 cgtgctcgcg cgcgagcggc agctgtcggt cgagcgcacg gccgatatct tgatccaggt
    11401 cggcgaggcg ctgatggagg cgcacgcgat cggctacgtc catcgcgacc tgcggccgcg
    11461 caacatctac ctcgcgaatc ggcgcggcaa ggcgaacttc gtcaagctgc tcgacttcgg
    11521 tctcgcgaag ctcgtcgagg gtgatgcgca agcagcgtcg acgagcctcg gcatgacgtt
    11581 cggcgatccg cgctacatgt cacccgagca agcgcgcggc gatcgcatcg accggcgcgc
    11641 cgacatctat cagctcggct gcatcgcgta cgagatgctc actggtgcgc cgccgtttac
    11701 cggcaatcgc gtgttcgaca tcctgtcgaa gcaggtcacc gagattcccg cgccgctacc
    11761 gacgcgacgt cccggcgtgc cgctgtggat ggaagcggcc gtcgcgaaga tgctggcgaa
    11821 ggatccggac aatcgatttg cgacgacgac gcgtctcgtc gaggcactac gccgcggcat
    11881 cgacaccggc gacgtgatgg aggacgagat cgcgcgtcgc cgc
//
LOCUS       DASUFX010000138         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1967971, whole genome shotgun sequence.
ACCESSION   DASUFX010000138 DASUFX010000000
VERSION     DASUFX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967971"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFV99_05495"
     CDS             complement(<1..600)
                     /locus_tag="VFV99_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5058792.1"
                     /translation="MARERLDKILIDRGLVASRERARALVMSGAVLVRGQPETKPGTM
                     LDPNVEITLREQDHPYVSRGALKLVKGLDTFAIDPTGKVALDIGASTGGFTDVLLRRG
                     ATRIYAIDVGYGQLAWQLRQDARVVVLERTNVRNMDLALVPEACDLAVIDVSFISLTL
                     VLPRVGELLRAHAAEGAATTGERAATAGKPIVALVKPQFE"
     gene            complement(729..1118)
                     /locus_tag="VFV99_05500"
     CDS             complement(729..1118)
                     /locus_tag="VFV99_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058793.1"
                     /translation="MKWLVLAIAFVAACKKQEAAPKQQRVTPMPAAEIQRSQDACKTY
                     VERICACAERAPANGTGSPGGSLAPDVAKQCELARALPEAVRIGLEVAASPDSQPDIV
                     QQSYASVRKTAKECIEQTAKLPALGCQ"
     gene            complement(1115..1909)
                     /locus_tag="VFV99_05505"
     CDS             complement(1115..1909)
                     /locus_tag="VFV99_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5058794.1"
                     /translation="MSTSFPLDNARVRFAGDTNIGRKRDHNEDSIALPETGERLAIVC
                     DGMGGHASGEVASRLAVELIVDHFTETGKQQTLTWPYKVDRDMRKDINRMVTGVMLAN
                     LEIWERSQREPRFKGMGTTCVGIYFLDDHLIIGHVGDSRCYRARGTEMIQLTEDHSLI
                     NDYIRMKRVTPEEAENWPHKNVIVRALGMKESVQVDIITEVPRVGDTYMLCSDGLTGM
                     LKDDTIQHILMSERDLDRAVDRLIQAANEEGGVDNISVVLARVEAS"
BASE COUNT          351 a          705 c          737 g          442 t
ORIGIN      
        1 ctcgaattgc ggtttgacga gcgcgacgat cggctttcct gccgttgcgg cgcgctcgcc
       61 cgtcgttgct gcgccctcgg ccgcatgtgc gcgcaacagc tcgcccacgc gcggtagcac
      121 gagcgtgagc gagatgaacg agacgtcgat gacagcgagg tcgcacgctt ccggcacgag
      181 tgcgaggtcc atgttgcgaa cattcgtccg ctcgagcacg acaacgcgcg cgtcttggcg
      241 tagctgccat gcgagctggc cgtagccgac gtcgatcgca tagatgcgtg tagctccgcg
      301 ccgcagcagg acgtcggtga agccgccggt cgacgcgccg atgtcgagcg cgaccttgcc
      361 agtcggatcg atggcgaacg tgtcgagccc tttgacgagc ttgagcgcgc cgcgcgagac
      421 gtacggatga tcctgttcgc ggagcgtgat ctcgacgttc ggatcgagca tcgtgccggg
      481 ctttgtctcg ggctgaccgc gcacgagcac ggcgccgctc atcacgagtg cgcgcgctcg
      541 ctctcggctc gcgacgagac cgcgatcgat caagatcttg tcgagacgct cgcgagccat
      601 cgctcgtgca atgtagcgct cgcggcgcag tgcgtgcgaa tcggcgatgc gattccgggc
      661 tgatgagatg acgcacagcg cgcggggtac gagcgcaccg agtccggctt cgtggcggcg
      721 atgcgcgact actgacaacc gagcgccggc agcttcgcgg tctgctcgat gcactctttc
      781 gccgtcttgc gcacgctcgc gtacgactgc tgcacgatat ccggctgcga gtccgggctc
      841 gcggcgactt cgaggccgat gcgcaccgct tcgggcaacg cgcgcgcgag ctcgcattgc
      901 ttcgccacgt caggcgccag cgagccgcca ggcgagccag tcccgttggc gggagctctc
      961 tcggcgcatg cgcagatgcg ctccacgtat gtcttgcacg cgtcctggct gcgctggatc
     1021 tcggcggccg gcatcggtgt cacgcgttgc tgcttcggcg cggcctcttg cttcttgcag
     1081 gccgcgacga acgcgatcgc gagcaccaac cacttcatga cgcctcgacg cgcgcgagca
     1141 cgaccgagat gttgtcgacg ccgccttctt cgttcgccgc ttggatcaag cgatcgaccg
     1201 cgcgatcgag gtcgcgctcg ctcatgagaa tgtgttggat cgtgtcgtcc ttgagcatgc
     1261 cggtcaggcc atccgagcac agcatgtacg tgtcaccgac gcgcggcacc tcggtgatga
     1321 tgtcgacctg cacgctctcc ttcatgccga gtgcgcgcac gatgacgttc ttgtgcggcc
     1381 agttctccgc ctcttccggc gtgacccgct tcatgcggat gtagtcgttg atcagcgagt
     1441 gatcctcggt gagctggatc atctcggtgc cgcgtgcgcg gtagcagcgg ctgtcgccga
     1501 cgtgcccgat gatgaggtgg tcgtcgagaa agtaaattcc gacgcaggtc gtacccatgc
     1561 ccttgaatcg gggttcccgt tgtgaccgct cccagatctc gaggttcgcg agcatgacgc
     1621 ccgtgaccat gcggttgatg tctttccgca tgtcgcgatc gaccttgtac ggccaggtca
     1681 gcgtttgctg tttgccggtt tcggtgaagt ggtcgacgat cagctcgacg gcaagccgac
     1741 ttgcgacctc gcccgaagca tgaccgccca tgccgtcgca cacgatcgcg aggcgttcgc
     1801 cggtctcggg cagcgcgatc gaatcctcgt tgtggtcgcg cttgcgaccg atgttcgtat
     1861 cgcccgcaaa tcgcacacgc gcgttatcga gcggaaacga ggtgctcacg cgcaaattct
     1921 accagtggct ggcggattga ctccactaca gcgggatggt cagttctagg ggaatgaagg
     1981 agccggcccc tgctgcggga tggtggggtt acgctgaagg caacgctact ctcgtcggaa
     2041 tcgcagtcgt aatcgtcgtg attgccgctg taatattcgt cgttcggaag tactggaggc
     2101 gttgaaacat gggtctgctt gctcgaggct cggtgaccga tcgcccgtgg ggaatgaccc
     2161 tcggtgcgct cggcatgcgt ggtctcaccg ggcagctcac cctcggcggc cagtacaagg
     2221 tcgcgttcca gcagg
//
LOCUS       DASUFX010000139         7491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_439500,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000139 DASUFX010000000
VERSION     DASUFX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439500"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFV99_05510"
     CDS             complement(<1..505)
                     /locus_tag="VFV99_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058795.1"
                     /translation="MLVAALCACGRIAFDTTANGDGGGGSGDTGGAGDGSGGSGDASG
                     DGGAPANWTVFSPSPSTASDLFAVASFAGNNVWIGGAAGITYQFDGTTWTPRTGPTVD
                     VRRFWGQSATDLWAVGVNCVVQRWDGNAWNGSTVTGCVSGAFFGIGGVAATDLWVGGA
                     GGRLNHLV"
     gene            complement(594..1541)
                     /locus_tag="VFV99_05515"
     CDS             complement(594..1541)
                     /locus_tag="VFV99_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058796.1"
                     /translation="MKHFWGLCLLLTGCILPISTGAPMPATTVGKGHIGGAMSGEAPV
                     LDLIADENAGAGSGGDPISYGAAPAAALTFTLSYGLGDNTDLEVAGEGALYYFILPLP
                     TGGSIGLRQHFDAGDALDIAVAARFGHVGSSSSVTDSNGNSTESSASANYGALQLVAQ
                     TKQGWVRPLLAINLMPSKIHRAPSEDPAYDFKGFASSVTGGLMLVGAHTVFGPYLAAT
                     NFYSDRFDNSGWFVSGGIMFAVRPDRNRPKPPPPPMYAPPPPMMGPPPPSAPPPMLPP
                     AGPPGDQPPGAAVPPNASPTAAPPAPQAPPVPTPTTTPM"
     gene            complement(1638..3686)
                     /gene="ligA"
                     /locus_tag="VFV99_05520"
     CDS             complement(1638..3686)
                     /gene="ligA"
                     /locus_tag="VFV99_05520"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5058797.1"
                     /translation="MAQMDLFGSRPTSSGGSKDEYLKLVEELSEHDRRYYVEAAPTIS
                     DVEYDKLLVKLREMEAAHPDWVVAWSPTKRVGYAPISEFPKVERAVAMLSLDNTYGHD
                     DLREFHDRVMKGLDGDSVSYSIEPKIDGFGIELTYKQGVLALGATRGDGRIGEDVTAN
                     VRTVRGVVLKLREPVDIVVRGEIYMTKAEFAAINAERIKEGEEPFKNPRNTAAGTIKQ
                     MDPREVAKRPLRTILYEVVDGERYAKGHGASLDYIKRLGLPVSPHNEQAASWDSLLAC
                     VEAWQAKRDELPYEVDGLVIKVDDFGQRAALGTTSKFPRWAIAYKFPARQVTTRLIDL
                     EPNIGRTGAVTPVAILDPVDVSGTTVSRASVHNYDQVLRLGLGRGDRVLIEKAGEIIP
                     QILGVTEKGPGPAFETPTQCPSCGGPLAKEQGKVALLCINRLGCPAQQLGAIEFFASR
                     GQMNIDGLGEKVVAQLVGAGMVKDVADLFVLEWEDIAELERFADQSAKNLVEAIAKAK
                     EAATFSRLLAALGIAHVGSVLAKPIAQKYGTLSGMRAACAAKDSAGFVAELCDIEGVG
                     ETTAEGIDRFLREPHVQAVLDKLAARGVDPGEPVVATSDGPLKGMTLVVTGTLSQPRT
                     DVQKKIEQAGGKVAGSVSKKTNYLVAGADTGKTKLEAAEKHGVKVIDETELEKLLAGS
                     "
     gene            complement(3707..4789)
                     /locus_tag="VFV99_05525"
     CDS             complement(3707..4789)
                     /locus_tag="VFV99_05525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5058798.1"
                     /translation="MSVQQKRTLKVEKLARIYDEEIAPVWGTRFGKMLLRNLAVPDRG
                     QVLDISCGTGYPTVEILRRMSDGSRLIAIDASSAMLDVARRKIADLGPLGKKGVFFRT
                     ESAVPKLSFADCVYDLVVCNLGLSEMPSIEIALRDFARVTKIGGEVRCTLPLAGTFEE
                     FHDLYREVLIKHDKHEALDRLERHIARYPTIEHVERCMKSANLAVDIEAEEFTLLFKS
                     SREFFFAPVIEYGPLQEWKDIAGNGQEMQDVFWYIKEAIDAYFDGRPFQVTVKAGCLI
                     GKRIEGAMLDVDEIELDLDGPSVEELDEEPDTDLPRRPYKMAAGSVRVDEVSEDDVID
                     EESPDEQALDAFIEGKKRPSHLDDDD"
     gene            complement(4862..6499)
                     /gene="thiC"
                     /locus_tag="VFV99_05530"
     CDS             complement(4862..6499)
                     /gene="thiC"
                     /locus_tag="VFV99_05530"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX5058799.1"
                     /translation="MSSKLPIVETVNVTPIAGFPSSEKVYVEESGLSVPMRRIHLDGG
                     EPPFDVYDTSGPQGHDPERGLPKLRQPWIDARLQSRSTHDGDDGNRSQMHYARRGIIT
                     PEMKFVAIRENVAPEFVREELARGRAILPANIKHPESEPMIIGKNFLVKINANIGNSA
                     VKSSIADEVDKLRWAVKWGADTVMDLSTGKQIHETREWIIRNSPVPIGTVPIYQCLEK
                     VGGDPAKLDIDVFIETLIEQAEQGVDYFTIHAGVRLAYIPYTAERVTGIVSRGGSIIA
                     KWCLSHHKENFLYTEFERICEVMKKYDVAFSLGDGLRPGSQADANDRAQLAELDTLGE
                     LTQIAWKHDVQTMIEGPGHVPMHKIKENVDIQMKVCHEAPFYTLGPLVTDIAPGYDHI
                     TSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKAGVIAYKIAAHAADLAKGHPGAI
                     ARDNALSKARFEFRWKDQFRLSLDPDTAQQFHDETLPAEGAKVAHFCSMCGPKFCSMK
                     LTEEVRELAAKNELETNLAAKAEEFRQGGGEIYVKNG"
     regulatory      complement(6490..6597)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6621..6992)
                     /locus_tag="VFV99_05535"
     CDS             complement(6621..6992)
                     /locus_tag="VFV99_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058800.1"
                     /translation="MLTVLVADDEFAVLEVLSMALEGEGHRVLKAGDGADALRILSGQ
                     PCDVIVCDENMPVMNGHQLIAAVRAEPKLDGTPIILMADTLGRPLPEIDGTVVLGKPI
                     LLSELFSHVERLGNSKARRKL"
     gene            complement(6992..>7491)
                     /locus_tag="VFV99_05540"
     CDS             complement(6992..>7491)
                     /locus_tag="VFV99_05540"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5058801.1"
                     /translation="IVEVMWQPALRYGLDQLAERLLDDVRKRKVRRVVIDGIDGFRQG
                     AAFPERTIRFVTALTNELRAVGTTVLITEETQKLFGPDVEVRIQGLSALVDNIILLEY
                     MDVGSELKRLLSVIKQRGSGHDGNVRELMISDRGIELATDSQTAHAILAGMFRGRETR
                     RLRDS"
BASE COUNT         1227 a         2525 c         2334 g         1405 t
ORIGIN      
        1 cgacgaggtg attgaggcgc ccgcccgcac caccgaccca caaatcggtc gcagcgacac
       61 cgccgatccc gaagaaggcg ccactcacgc agccggtcac tgtcgatccg ttccacgcgt
      121 tgccatccca gcgctggacg acacagttca cgccgaccgc ccacagatcc gttgccgact
      181 gtccccagaa tcgcctgaca tcaacagtcg gaccggtgcg cggtgtccac gtggtgccgt
      241 cgaactggta cgtgatgccg gccgcaccac cgatccagac attgttgccc gcaaagctcg
      301 cgaccgcgaa tagatcgctc gcggtgctcg gcgacggcga gaacacggtc cagttagcgg
      361 gagcgccgcc atcgccagag gcatcgccgc tgccgccact tccgtctccg gcgccaccgg
      421 tgtcgccgct gcctccgcct ccgtcgccgt tcgctgtcgt gtcgaaggcg atcctgccgc
      481 atgcgcacag cgcggcaacg agcatgaccc caaacgaacg acgcaccatc tccacagtac
      541 ctcgaacggt gtggcaccgg agcaccgacg aaatgcaagc tctgcgcgct cgcttacatc
      601 ggcgtcgtcg ttggtgtcgg caccggtggt gcttggggtg ccggcggggc agcggtcggt
      661 gaagcattcg gtggcaccgc cgcgcccggt ggttggtcgc ccggtggccc cgcgggcggc
      721 agcatcggcg gcggcgcgct cggcggcggc ggtcccatca tcggcggcgg tggtgcgtac
      781 atcggcggcg gtggcggctt cggccgattc cgatcgggcc gcaccgcgaa catgatgccg
      841 cccgatacga accagccgct gttgtcgaag cgatcgctgt agaagttcgt cgctgcgagg
      901 tacggaccga acacggtgtg agcgccgacg agcatcagcc cgccggtgac cgacgacgcg
      961 aagcctttga agtcgtatgc cgggtcctcg ctcggcgcac gatggatctt cgagggcatc
     1021 aggttgatcg cgagcagcgg ccgcacccag ccctgcttcg tttgcgcgac cagctgaagc
     1081 gcgccgtagt tcgccgaggc gctactctcg gtggaattgc cattcgagtc ggtcaccgac
     1141 gagctgctgc cgacgtgacc gaagcgcgct gcgacggcga tgtcgagtgc gtcgcccgcg
     1201 tcgaagtgct ggcgcaagcc aatcgagccg ccggtcggca acggcaagat gaagtaatag
     1261 agcgcgcctt cgccggcgac ctcgagatcg gtgttgtcgc cgagtccgta gctcagcgtg
     1321 aacgtcagcg ccgccgcggg cgccgcgcca tacgagatcg gatctccacc gctgccggcg
     1381 ccggcattct cgtcggcgat cagatcgagg accggcgctt caccactcat cgctccgccg
     1441 atgtgacctt taccgacggt cgtcgccggc atcggcgctc ccgtcgaaat cggcaagatg
     1501 cagccggtca gtagtaagca cagtccccag aagtgcttca tacggccatt ggatcatggt
     1561 ttggcgccat gcgatagcgt ccgcggctca aacgccgacg aatgccacgt gcagtgaccg
     1621 ctgctggatc gtgagattca gcttcccgcg agcagcttct cgagctcggt ctcgtcgatc
     1681 accttgacgc cgtgcttctc tgcagcctcg agcttggtct tgcccgtgtc ggcaccggcg
     1741 acgagatagt tcgtcttctt cgacaccgag ccggcgacct tgccgcccgc ctgttcgatc
     1801 ttcttctgca catcggtgcg cggctgcgac agcgtgccgg tcacgacgag cgtcatgccc
     1861 ttgagcggac cgtcgctggt agcaacgacc ggctcgcctg gatcgacgcc gcgcgctgcg
     1921 agcttgtcga gcacggcttg cacgtgcggc tcgcgcagga agcgatcgat gccctcggcg
     1981 gtcgtctcac cgacgccctc gatgtcacac agctcggcga caaaccccgc tgaatccttc
     2041 gcggcgcacg cggcgcgcat tccggagagc gtgccgtact tctgcgcgat gggctttgcg
     2101 agcacgctgc cgacgtgtgc gatgccgagc gcggcaagca ggcgcgaaaa cgtcgccgcc
     2161 tccttcgcct tcgcgatcgc ctcgacgagg ttcttcgccg attgatcggc aaaccgctcg
     2221 agctcggcga tgtcctccca ctcgagcacg aacagatcgg cgacgtcttt gaccatcccc
     2281 gcgccgacga gctgcgcgac gaccttctcg ccgaggccat cgatgttcat ctgaccgcgc
     2341 gatgcaaaga actcgatcgc gccgagctgc tgcgccgggc aaccaagccg gttgatgcag
     2401 agcagcgcga ccttgccctg ctctttcgcg agcgggccgc cacacgacgg acactgcgtc
     2461 ggcgtttcga acgccgggcc tggccccttc tcggtgacac cgaggatctg cgggatgatc
     2521 tcgccagcct tctcgatcag cacgcggtcg ccgcgcccga ggccgagccg cagcacctga
     2581 tcgtagttgt gcacgctcgc acgcgagacc gtcgtcccgc tgacgtcgac cggatcgagg
     2641 atcgcgaccg gcgtgacggc acccgtgcga ccgatgttcg gctcgaggtc gatgagacgc
     2701 gtcgtgacct ggcgcgccgg aaacttgtac gcgatcgccc agcgcggaaa cttcgacgtc
     2761 gtgccgagcg ctgcccgctg gccgaaatcg tcgaccttga tcacgaggcc atcgacctcg
     2821 tagggcagct cgtcacgctt cgcctgccac gcctcgacgc acgcgagcag cgagtcccag
     2881 ctcgccgcct gctcgttgtg cggcgacacc ggcagtccga gccgcttgat gtagtcgagc
     2941 gacgcgccgt ggcccttcgc atagcgctcg ccgtcgacga cttcatagag gatcgtgcgt
     3001 agcggccgct tcgcgacctc gcgcggatcc atctgcttga tcgtaccggc cgccgtattg
     3061 cgcgggttct tgaacggctc ctcgccctcc ttgatgcgct cggcgttgat cgcggcgaac
     3121 tccgccttcg tcatgtagat ctcgccgcgc acgacgatgt cgaccggctc gcgaagcttg
     3181 agcacgacgc cgcgcaccgt gcgcacgttc gcggtcacgt cctcgccgat gcggccatca
     3241 ccgcgcgtgg cgccgagcgc gagcacgcct tgcttgtacg tcagctcgat gccgaagccg
     3301 tcgatcttcg gctcgatcga gtacgacacg ctgtcgccgt cgaggccctt catcacgcgg
     3361 tcgtggaact cgcgcagatc gtcgtggcca tacgtgttgt cgagcgacag catcgcgact
     3421 gcacgctcga ccttcggaaa ctcagaaatc ggcgcatagc cgacgcgctt cgtcggcgac
     3481 cacgcgacca cccagtcggg atgcgcggcc tccatctcgc gcagcttgac cagcagcttg
     3541 tcgtactcga cgtcgctgat cgtcggcgcg gcttcgacgt agtaccggcg gtcgtgttcg
     3601 ctcagctctt cgacgagctt cagatactcg tctttcgagc ccccactgct cgtcggccta
     3661 ctcccgaaca gatccatctg cgccataacc ggcgcagcat agcggatcag tcgtcatcat
     3721 cgaggtgcga cgggcgcttc ttgccttcga tgaaggcgtc gagcgcctgc tcgtcggggg
     3781 attcctcgtc gatcacgtcg tcttcggaga cttcgtcgac gcggaccgag cccgccgcca
     3841 tcttgtacgg ccggcgcggc aagtcggtat ccggttcctc gtcgagctct tcgaccgacg
     3901 ggccatcgag atcgagctcg atctcgtcga cgtcgagcat cgcgccctcg attcgcttgc
     3961 cgatcagaca gccagccttg acggtgacct ggaacggccg gccatcgaag tacgcgtcga
     4021 tcgcttcctt gatgtaccag aagacgtcct gcatctcctg gccgttgccg gcgatgtctt
     4081 tccactcctg gagcggtccg tactcgatca ccggcgcgaa gaagaactcg cgcgagctct
     4141 tgaacagcag cgtgaactct tcggcttcga tgtcgacggc gaggttcgcg ctcttcatgc
     4201 agcgctcgac gtgctcgatc gtcggatagc gcgcgatgtg gcgttcgaga cgatcgagcg
     4261 cctcgtgctt gtcgtgcttg atcagcactt cccgatagag gtcgtggaat tcctcgaacg
     4321 tgccggcgag cggtaacgtg cagcgcactt cgccgccgat cttcgtcacg cgcgcgaagt
     4381 cgcgcagcgc gatctcgatg ctcggcatct cggagagccc gagattgcag accacgaggt
     4441 cgtagacgca gtcggcaaac gacagcttgg gcaccgcgct ctcggtgcgg aagaagacgc
     4501 ccttcttacc gagcggcccg aggtccgcga tcttgcggcg cgcgacatcg agcatcgcgc
     4561 tcgacgcatc gatcgcgatc aagcgcgagc catcactcat ccgtcgcaag atctcgacgg
     4621 tggggtaacc ggtgccacac gaaatatcga gcacctgtcc gcgatctggt accgcgaggt
     4681 tccgcagcag catcttgccg aaacgagtgc cccaaacggg cgctatctct tcgtcataga
     4741 tgcgcgccag cttctctact ttgagcgtgc gcttttgctg aaccgacacg acggatacta
     4801 ggtacccgga atcatgacgg aacggtgacg ggatgtctac actcaggttc tagctgcctg
     4861 attacccatt cttcacgtag atctcgccgc cgccctgccg gaactcttcg gctttcgccg
     4921 ccaggttggt ctcgagctca ttcttggctg ctagctcgcg aacttcctcg gtgagcttca
     4981 tcgagcagaa cttgggaccg cacatcgagc agaaatgcgc gaccttagct ccctcggcag
     5041 gcagggtctc atcgtggaat tgctgcgctg tgtcggggtc gagcgacagc cggaactggt
     5101 ccttccagcg gaactcgaag cgcgccttcg acagggcgtt gtcacgggcg atcgccccgg
     5161 ggtgtccctt ggcgagatcg gccgcgtgcg ccgcgatctt gtaggcgatc acgcccgcct
     5221 ttacgtcgtc tcgatcgggc agaccgaggt gctccttcgg cgtcacgtag cagagcatcg
     5281 cggtgccgaa ccaaccgatc atcgcagcgc cgatcgcgct cgtgatgtgg tcgtagccgg
     5341 gcgcgatgtc cgtgaccagc gggccgagcg tgtagaacgg cgcctcgtgg cagaccttca
     5401 tctggatgtc gacgttctct ttgatcttgt gcatcggcac gtggccgggg ccttcgatca
     5461 tcgtctggac gtcgtgcttc caggcgatct gcgtgagctc gccgagcgtg tcgagctccg
     5521 caagctgcgc gcgatcgttc gcgtcggcct gcgagccggg acgtagaccg tcgccgagcg
     5581 agaacgcgac gtcgtacttc ttcatgacct cgcagatgcg ctcgaactcg gtgtagagga
     5641 agttctcctt gtggtgcgag aggcaccact tcgcgatgat cgagccgccg cgcgacacga
     5701 tgccggtcac gcgctcggcg gtatacggga tgtacgcgag ccgcacgccc gcgtgaatcg
     5761 tgaagtagtc gacgccctgc tccgcttgct cgatcaacgt ctcgatgaag acgtcgatat
     5821 cgagcttcgc cggatcaccg ccgactttct cgaggcactg atagatcggc accgtgccga
     5881 tcggcaccgg cgagttgcga atgatccact cgcgcgtctc gtggatctgc ttgccggtcg
     5941 aaaggtccat gaccgtgtcg gcgccccact tgacggccca ccgcagcttg tcgacctcgt
     6001 ctgcgatcga cgacttaacg gcgctgttgc cgatgttcgc gttgatcttc acgaggaagt
     6061 tcttgccgat gatcatcggc tcggactcgg ggtgcttgat gttcgcgggc aggatcgctc
     6121 gaccgcgtgc gagctcctca cgcacgaact ccggcgcgac gttctcgcga atcgcgacga
     6181 acttcatttc cggcgtgatg atgccgcgcc gtgcgtagtg catctgcgag cggttgccgt
     6241 cgtccccgtc gtgggtggag cgcgactgaa gtcgcgcatc gatccacggc tggcgcagct
     6301 tcggaaggcc gcgctccgga tcgtgaccct gcgggcccga cgtgtcgtag acatcgaacg
     6361 gcggctcacc accgtcgagg tggatgcggc gcatcggcac cgagagaccg ctctcttcga
     6421 cgtacacctt ctcggagctg ggaaagcctg cgatcggcgt gacgttgacg gtttcgacga
     6481 tcggtaactt gctgctcatt gcttcctccc tacgccggca ttacccgggt caggttctgg
     6541 ggtcgcacgt tgccgtgcct ctcagcctcc tcgtcggaag ctcccccgtt atgtgcggga
     6601 tttgcggacc ctaccccagc tcagagcttc cgtcgagcct tactgttgcc gagccgttcg
     6661 acatggctga acagctccga tagcaggatc ggcttaccga gaactacggt gccgtcgatc
     6721 tccggcagcg ggcgacctaa cgtatccgcc atcaggatga tgggagtgcc atcgagcttt
     6781 ggctcggcgc gtacggccgc gatcagctgg tggccgttca tcaccggcat gttttcgtca
     6841 cagacaatta catcgcaggg ctggccggac agaatgcgca acgcatccgc gccatcgccg
     6901 gctttcagca cgcgatgccc ttcgccttcc agtgccatcg acagcacttc gagcacagcg
     6961 aattcgtcgt ctgcgacgag gaccgtcagc atcagctgtc gcgcagcctc cgggtttcgc
     7021 gacctcgaaa catgcccgcc aagatcgcgt gtgccgtctg cgagtccgtc gcgagctcga
     7081 tgccgcggtc gctgatcatc agctcgcgca cgttgccgtc gtggcccgag ccgcgctgtt
     7141 tgatcacgga cagcaagcgc ttcagctcgg acccgacgtc catgtactcg agcaagatga
     7201 tgttgtcgac gagcgccgag aggccctgga tgcgaacctc gacatcgggg ccgaacagct
     7261 tctgcgtctc ctcggtgatg aggaccgtcg tgccgaccgc ccgcagctcg ttcgtcagtg
     7321 ccgtgacaaa ccggatcgtg cgctcgggga acgcggcgcc ctgccggaat ccgtcgatac
     7381 cgtcgatcac gacgcgacgc accttgcgct tccgcacgtc gtcgagcagc cgctcggcga
     7441 gctgatcgag tccgtaccgc agcgcgggct gccacatcac ctcgacgatg c
//
LOCUS       DASUFX010000140        10814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_856816,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000140 DASUFX010000000
VERSION     DASUFX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856816"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..680)
                     /locus_tag="VFV99_05545"
     CDS             complement(162..680)
                     /locus_tag="VFV99_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5058802.1"
                     /translation="MKRELVVTADGRDRTVTVEGPLPEAPGRFRVTIDGTAHEVDGRA
                     IRPGTWSVVVGGRSFLVDLDRRRAGIAASVGASEAMLQVEDALHRRLAAAAQPRGAAR
                     GETIRAPIAGKVVKVLVAVGDNVTPGTPVIVLEAMKMENELVAERGGTVKTVNKQAGQ
                     AVDTGDLLIDIE"
     gene            714..1865
                     /locus_tag="VFV99_05550"
     CDS             714..1865
                     /locus_tag="VFV99_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5058803.1"
                     /translation="MPKLVDVLLERRIIVCVGSGGVGKTTTAATLALAAARRGKRTLV
                     LTIDPAKRLANSLGLEALGHQVQEVPPALIRDGAPSDRGQLHAMMLDQKAAFDEMVQR
                     HARDPVAVKRILTNPVYAQISGSLSGAQEYAAMAKLHDFDATGEWDTIVVDTPPTAHA
                     LDFLDAPRKLSEAIDSPAIEWFRKLQGGSGSGWSLVGKTGAFVLKRLAKFVGSQFIDD
                     LGVFFTEFNDILGGFRKRAEETFSLLRQPRVGFVLVASPEPMAVREALFFHERLRSAQ
                     MPFVGFVINKVHPALPIRADEPAIAAALGHQASVAALGLSATTRTMAAQALFAAHGEL
                     ETLAEADRMAVARMRDAGGANALVIQVPLLRDDVHDVSRLVGLERFLLA"
     gene            complement(2160..3446)
                     /locus_tag="VFV99_05555"
     CDS             complement(2160..3446)
                     /locus_tag="VFV99_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058804.1"
                     /translation="MATLAVLLVSVAAHADTDPLAALLARTDAIAKQVSKQRGLPIKK
                     KVEQEVVDRDELRARLIKLAAEQKTQTETRAEGLALARWGMIPLDTDYLQLMIDLLTD
                     QIAGYYDPDTKKLTISKTAGSDPDWAEMVLAHELDHALQDQTYDLDKFEAVPDTEGDA
                     ALARQALVEGDGVALMIELMLARKKMDPPWRDPGVARELSLAMALPSGDSLDKAPLAV
                     REALLFPYRAGFTFIAALRRKQPWSAVDAAFKRPPRSTEQILHPERYQADEKPIVVDI
                     KPPMALAEYHVTHSSVWGELGFSLFVRSHGLDERAGAIAADGWGGDRVITLTRGDDQK
                     PEHAIGIARFEWDSEPDAIEAHEAVVRALDDAILGATVEHSETRTRWLALDGRVTIVE
                     RRGTSIAIAHGVPLSLLETVQTDLWTMTSVVAAAKP"
     gene            3527..5524
                     /locus_tag="VFV99_05560"
     CDS             3527..5524
                     /locus_tag="VFV99_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5058805.1"
                     /translation="MWTRITRFVPLTLLFALLVIPRSSRADDDDKSLSPYFFVEGAKP
                     GVEAMPLESTKADVHITGTIADVTVKQTYRNDGDRTISAKYVFPASTRAAVYGMQMKI
                     GSRVIVAKIKEREEARKEYEEAKAAGKTASLLEQDRPNVFSMNVSNILPKDRIEVELR
                     YTELLVPTDGVYELVYPTVVGPRYASETLDAKKADNKFVSSPYQHAGDTPTYAFDINV
                     SVAAGMKIQQLDSPSHPIKTAFGANNMNATVALDGGKAQGNKDFVLHYRLAGDDISSG
                     LLLFPGAKENFFLMMVQPPRRPAIEQIPPREYVFIIDVSGSMSGFPLDTTKVLMKDLL
                     GKLRPTDHFNVLLFAGGSSLYKDKSVPAEADKISDAIAFIDRETGGGGTELLPALQRA
                     MKLPVLDKNVSRTFVVVTDGYIAEETAMFEQVRSHLGEANVFAFGIGSSVNRFLIDGI
                     AKAGQGEPFVVLDQSKADTAAAKFRAYVEQPVLTQVKVAFDGMQTYDVEPKVLPDVFA
                     NRPVVVFGKYKGSPTGKITLTGVSGRGRFVNTIDASTVSPDAKNSALQYLWARTKISE
                     LSDFFQGDANKKEVVALGLQYNLLTKFTSFIAVQQIVRTDTGSTDVDQPLPMPAGVSD
                     LAVASGEMEVGAEPPLLVLIGLVALGMTIAMFRTYRRRRAA"
     gene            5521..6234
                     /gene="xrtK"
                     /locus_tag="VFV99_05565"
     CDS             5521..6234
                     /gene="xrtK"
                     /locus_tag="VFV99_05565"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase K"
                     /protein_id="HEX5058806.1"
                     /translation="MSFSLETGSRVLREHRWRIAAVVVALAVVVAGKQYYRAASANDL
                     RWILAPTAQLVSWVTGHEFAYEAGQGWMNTDVMFVIAPACAGVNFALAAFLALVLGGL
                     AGMTSLPATAKRFGFAVLLAYVATLVVNTTRISIAVDMHRADLHRLEGIVVYLVGLCG
                     LYAVARALDGRKLRSLNWLAIPLCTYVMITLVMPLANGAAQRDDFVRHATTVLAVCVV
                     MVAIASFCELVRWSWRRKQ"
     gene            6231..6866
                     /locus_tag="VFV99_05570"
     CDS             6231..6866
                     /locus_tag="VFV99_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058807.1"
                     /translation="MKAWLIPFSLVVLADTAHADQCAWIDKPTAQKAEAIVLKASKYI
                     EFCEPCGEKAPGIPQKAQSVAVQDADGDYKEVTINGKGVDLAYTFVKTDAGHYRNLAA
                     LAGCPAEGVSPSLAIEDETPNGVMIRAADDTLRPQPPPPQPLPAPAAAPPQLQVPPQV
                     YVYNTTRDIAWLPIALAACGGFVTGSALTLLVFAARRRRAMRPRAVDIAAR"
     gene            complement(6868..7596)
                     /locus_tag="VFV99_05575"
     CDS             complement(6868..7596)
                     /locus_tag="VFV99_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058808.1"
                     /translation="MTDEPRFLRSGDSAAEPGASGDKPAPQIEDTPERRALLEKVRAL
                     REQREVLEAELTQRLDEHSRKCIGCDSTDTSPAYVLVNYGSHEIARRYSIVGTVSIQS
                     STLAPSVSPMCERCYARVAANRQRHRIGAAVAAVGIVIGMGYFVAALAKASTAVSLGL
                     LFGGGALMALAMVHAVFNLRSLHHQLKLPKLTGMAVLLVTRKRDELERLLDRCELNSR
                     ELQSQIDRKASDAAALPAARIHPK"
     gene            complement(7609..9333)
                     /locus_tag="VFV99_05580"
     CDS             complement(7609..9333)
                     /locus_tag="VFV99_05580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058809.1"
                     /translation="MTDTHALDGENLTKVNDDEQPVDVEDTPLVSDAERFDSIGLLGK
                     GGMGEVRLMRDRRISRYVAYKVLRAGVENDPGYRARFLLEARVQGQLEHPAIVPVHDL
                     GETAGGELYFAMKCVRGRTLRQAIDSLAAGVRTPQYSRRRLLTAFSSVCLAVDFAHAR
                     GVVHRDLKPENVMLGQFGEVYVLDWGIAKVMHRGETPLADTIDVGIGTNATKAGSTLG
                     TPKYMSPERQLGVANPQTDVFALGVILGEILAAHSEHDVPPELETIQMRASALDAGGR
                     YPTARALHEALEQFLDGDRDLETRKRVSEEHARRAAEVLARADHDPASRTIAGQEIGR
                     ALGLDPDNRLALHTLMRMLTDVPAVLPPAAKAEMDRRWTDRQRRQLRRATLPTAMMLL
                     AVPLILWLGVRNWPLFGAYVGLVLLATALQYVSANLTRPTAGPAQFATVAASAVLMTS
                     FGLLGGVPAALAIIALGWRMNVDKAIHGLLILVFIGLWLVAPFVLERLGLQSPMYEVR
                     DGAIVLLPKMNAFPPGRTFVAMVLTTFGAIGAAVIWGRLFINELRRAEHKLSFQAWQL
                     QQLVPPER"
     gene            complement(9359..10111)
                     /locus_tag="VFV99_05585"
     CDS             complement(9359..10111)
                     /locus_tag="VFV99_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5058810.1"
                     /translation="MRSYVVLVLAGCGFQHGTATEDAAIPDAPDANVDMMIDAPAPFC
                     AADPHLRLCYSFDQDPLPPSLANEGAANVSATLTNVTHTARGATGAAQIDSTSMIYVP
                     YTAEVTNIQGFEVSFRADGAPAINGARLGILDTNVIPPNVSFFLYRVDPGYQLRCGLG
                     GALYAFDAPLVLGMWHDAVCTCTADTLQIYLDGAKIGEQATSGCSSGGGFVSAGMTIG
                     QNNNGGPTGADEWLVGAVDNVRLWDVPVAPPQ"
     gene            10170..>10814
                     /locus_tag="VFV99_05590"
     CDS             10170..>10814
                     /locus_tag="VFV99_05590"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX5058811.1"
                     /translation="MRTVRATRYLTPLREGGSVPAIIEADDDGTYVAKFRGAAQGEKA
                     LIAEIICGELARLLGLPVPQIVHVDIDLLLSRSEPDQELQELLRKSAGTNVALDYLPG
                     ALNWEPPLAPPPDRDLAAQIVWFDALITNVDRTVKNPNMLIWHRRLHLIDHGAALYFH
                     HQWDDHLARAKGAFPAIRTHALLPLAGDLAAADAALAPKIDDAGLRAILADVPDE"
BASE COUNT         1790 a         3643 c         3559 g         1822 t
ORIGIN      
        1 gcaactcgtc gctcggcctg ctcggcatgc gcgagcgtgc gcgccgcgcc ggtggcgact
       61 gcacgatccg ccgccgcgat ccgcggggca cgatcgtcgc gctctcggtg ccgctccgct
      121 ttccagccga gcacgatcac gatcacgagc tactcgcgta gctattcgat gtcgatcagc
      181 agatcgccgg tatcgacggc ctgaccggcc tgcttgttca cggtcttgac cgtgccgccg
      241 cgctccgcga cgagctcgtt ttccatcttc atcgcttcga gcacgatcac cggcgtgccc
      301 ggcgtgacgt tgtcgccgac cgcgacgagc actttgacga ccttgccggc aatcggtgca
      361 cggatcgtct caccgcgagc ggctccacgc ggctgcgcag cggccgcaag gcggcgatgc
      421 aacgcgtctt cgacctgaag catcgcctcg ctggcgccga ccgatgccgc gattcccgcg
      481 cgccggcggt cgagatcgac caggaagctg cgaccaccca ccacgaccga ccacgtcccc
      541 ggccggatgg cacggccatc gacctcgtga gcggtgccgt cgatggtgac gcggaagcgc
      601 ccaggggcct ccggaagcgg gccctcgacc gtgaccgtgc ggtcgcgccc gtcggcggta
      661 acgactagct cgcgcttcat gtgcgcctac ttatatgcta tccgcagtcg aacgtgccga
      721 agcttgtcga cgttttgcta gagcggcgca tcatcgtgtg tgttggctcg ggaggcgtgg
      781 gcaagaccac gaccgctgcg acgctggcac ttgccgccgc acgccgcggc aaacgcacgc
      841 tcgtgctcac gatcgacccg gcgaagcggc tcgcgaattc gctcggtctc gaagcgctcg
      901 gccatcaggt gcaagaggta ccgcccgcgc tgatccgcga cggtgcgccg agtgatcgcg
      961 gtcagttgca cgcgatgatg ctcgatcaga aagccgcatt cgacgagatg gtgcagcgtc
     1021 acgccagaga tccggtggct gtgaaacgca ttctcacgaa tccggtttac gcgcagatct
     1081 ctggttcgct gtcgggcgcg caggagtacg cggcgatggc gaagctgcac gacttcgacg
     1141 cgaccggtga atgggacacg atcgtcgtcg acacgccgcc gaccgcgcac gcgctcgact
     1201 tcctcgatgc gccgcgcaag ctgtccgaag cgatcgactc gccggcgatc gagtggtttc
     1261 gcaagctgca aggcggctcg ggctcggggt ggtcgctcgt cggcaagacc ggcgcgttcg
     1321 tgctcaagcg gctcgcgaag ttcgtcggct cgcagttcat cgacgacctc ggcgtgttct
     1381 tcaccgagtt caacgacatc ctcggcggtt tccgcaagcg cgccgaagag acgttctcgc
     1441 tgttgcggca gccgcgcgtc ggcttcgtac tcgtcgcgag ccccgagccg atggccgtgc
     1501 gcgaagcgct gttcttccac gagcggttgc gatcggcgca gatgccgttc gtcggcttcg
     1561 tgatcaacaa ggtgcacccc gccctgccga tccgcgccga cgagcctgcg atcgcagctg
     1621 cgctcggcca ccaggcaagc gtcgccgcgc tcggtctatc ggcgacgacg cgcacgatgg
     1681 cggcgcaggc gttgttcgcc gcccacggcg agctcgagac gctggccgaa gccgatcgga
     1741 tggcagtcgc acgcatgcgc gacgccggtg gcgcgaacgc gctcgtgatc caggtgccgc
     1801 tcttgcgcga cgacgttcac gacgtgtcgc ggctcgttgg cctcgagcgc ttcctgctcg
     1861 cgtagacctc gacagcgcga cgcacgatgg cgtgcgcact ggactggccg tccttggagc
     1921 ggcgattggc gcctgcgacg ccgcacgacg tgttccagat ctaacgccaa gaacgccaag
     1981 cgaagcccgc cgagaacgcc accggaacgt gggccttcgc agtggtctgc acgcgcggtc
     2041 gtgcgagcag aacgcgatcc gaaacccttc ttctggcgtt cttggcggct tggcgccttg
     2101 gcgttcctcc ggggccgcga agcgaacgcg ggctcctgcg acgctgtgcc caacgcacgc
     2161 tagggcttcg ccgcggcgac gacgctcgtc atcgtccaca ggtcggtctg gacggtttcg
     2221 agcaacgaca gcggcacacc gtgcgcgatc gcgatcgacg tgccgcgacg ctcgacgatc
     2281 gtgactctgc cgtcgagtgc gagccagcgc gtgcgggtct cgctgtgctc gacggtggca
     2341 ccgaggatcg cgtcgtcgag cgcgcgtacg accgcttcgt gggcctcgat cgcatcgggt
     2401 tcgctgtccc actcgaagcg cgcgatgccg atcgcgtgct cgggcttctg atcgtcgcct
     2461 cgcgtcaacg tgattacgcg atcgccgccc cagccgtcgg ccgcgatcgc gccggcgcgc
     2521 tcgtcgagac catgcgaacg aacgaacagc gagaagccga gctcgcccca tacgctcgag
     2581 tgcgtgacgt ggtactcggc cagcgccatc ggcggcttga tgtcgacgac gatcggcttc
     2641 tcgtccgcct ggtatcgctc gggatgcagg atctgttcgg tcgaccgcgg cggccgcttg
     2701 aatgcagcgt cgaccgccga ccacggctgc ttgcggcgca gcgccgcaat gaacgtgaac
     2761 ccagcgcgat acggaaacag caacgcttcg cgcactgcga gcggcgcttt atcgagcgag
     2821 tcgccgctcg gcagcgccat cgccagtgac agctcacgag cgacgcccgg atcgcgccag
     2881 ggcgggtcca tcttcttgcg cgcgagcatc agctcgatca tgagcgcgac accgtcgcct
     2941 tcgacgagcg cctggcgagc gagcgccgcg tcgccctcgg tatccggcac ggcttcgaat
     3001 ttgtcgaggt cgtacgtctg gtcctgcagc gcgtgatcga gctcgtgcgc gagcaccatc
     3061 tcggcccagt ccggatcgga gccggccgtc ttcgagatcg tcagcttctt ggtatcggga
     3121 tcgtagtagc cagcgatctg atcggtgagc agatcgatca tcagctgcag gtaatcggta
     3181 tcgagcggga tcatgcccca gcgcgcgagt gcgagcccct cggcacgcgt ctcggtctgt
     3241 gtcttctgtt cggcagcgag cttgatcaag cgagcgcgca gctcgtcgcg atcaacgacc
     3301 tcttgctcga ccttcttctt gatcggcagt ccgcgctgct tggacacctg cttcgcgatc
     3361 gcatcggtgc gggcgagcaa agccgcgagt ggatcggtat cggcatgtgc agccacgctg
     3421 acaagcagca ccgcgagcgt tgccacaaaa gtagtggccc gcgagacacg ctcgcagcgg
     3481 tttcgacgag atgcgcgtgg cactgccatt gctctagcac gccttcatgt ggaccaggat
     3541 cacgcgtttc gtacctctca cgcttctctt cgcgctgtta gtcattcctc gctccagccg
     3601 tgcggatgac gacgacaagt cgctttcccc ctacttcttc gtcgagggcg ctaagcccgg
     3661 cgtcgaagcg atgccactcg agagcacaaa ggcagacgtc cacatcaccg gcacgatcgc
     3721 cgatgtgacg gtcaagcaga cgtataggaa tgatggcgat cgaacgatca gcgccaagta
     3781 tgtattcccg gcatcgacgc gcgccgcggt ctacggcatg cagatgaaga tcggcagtcg
     3841 cgtcatcgtc gcgaagatca aggaacgcga ggaggcccgt aaggaatacg aggaggcgaa
     3901 ggccgccggt aagaccgcgt cgctgctcga gcaggaccgg ccaaacgtgt tctcgatgaa
     3961 cgtctcgaac atcctgccca aggaccggat cgaggtcgag ctccgctaca ccgagctgct
     4021 cgtgccgacc gacggcgtgt acgagctcgt gtatccgacc gtggtcggcc cgcgctacgc
     4081 gagcgagaca ctcgatgcca agaaggccga caacaagttc gtcagctcgc cgtatcagca
     4141 cgccggtgac accccgacct acgcgttcga catcaacgtc agcgttgccg cgggcatgaa
     4201 gatccagcag ctcgactcgc cgtcgcatcc gatcaagacg gcgttcggcg cgaacaacat
     4261 gaacgcgacc gtcgcgctcg acggtggcaa ggcgcaaggc aacaaggact tcgtgttgca
     4321 ctatcgcctc gcgggcgacg acatctcgtc gggcctgctg ttgttcccgg gcgcgaagga
     4381 gaacttcttt ctgatgatgg tgcagccacc gcgccgcccc gccatcgagc agattccgcc
     4441 acgcgagtac gtgttcatca tcgacgtgtc gggttcgatg agcggcttcc cgctcgacac
     4501 cacgaaggtt ctgatgaagg acctactcgg caagctgcgc ccgaccgatc acttcaacgt
     4561 gctcctgttc gcgggtggct cgtcgctgta caaggacaag tcggtaccgg ccgaggcaga
     4621 caagatcagc gacgcgatcg cattcatcga tcgcgagacc ggtggtggcg gaaccgagct
     4681 gctccccgcg ctgcagcgcg cgatgaagct gccggttctc gacaagaacg tgtcgcgcac
     4741 gttcgtcgtg gtgaccgacg gctacatcgc cgaggagacc gctatgttcg agcaggtgcg
     4801 ctcgcacctc ggtgaagcaa acgtgttcgc gttcggcatc ggcagctcgg tcaaccgctt
     4861 cctgatcgat ggcatcgcga aggcgggcca aggcgagccg ttcgtcgtgc tcgaccaatc
     4921 gaaggccgac accgcagcgg cgaagttccg cgcgtatgtc gagcagccgg tgctgacaca
     4981 ggtcaaggtc gcgttcgacg gcatgcagac ctacgacgtc gagccgaagg tgctgcccga
     5041 cgtgttcgcg aaccgccccg tcgtcgtgtt cggtaagtac aagggttcgc cgaccggcaa
     5101 gatcacgctc accggcgtgt cgggccgcgg ccgcttcgtg aacacgatcg acgcgagcac
     5161 ggtgtcgccc gacgcgaaga actcggcgct gcagtacctg tgggcgcgca ccaagatctc
     5221 ggagctctcg gacttcttcc agggcgacgc gaacaagaag gaagtcgtgg cgctcggcct
     5281 ccagtacaac ctgctgacca agttcacctc gttcatcgcg gtccagcaga tcgtgcgcac
     5341 cgacaccggt tcgaccgacg tcgaccagcc gctgccgatg cccgcgggcg tcagtgacct
     5401 cgcggtcgca agcggcgaga tggaagtcgg tgccgagccg ccgctgcttg tcctgatcgg
     5461 cctcgtcgcg cttggcatga cgatcgcgat gttccgcacg tatcgccgcc ggagggctgc
     5521 gtgagctttt cgctggagac cgggtcgcgc gtgctgcgcg agcatcgttg gcggatcgcg
     5581 gcggttgtcg tcgcgctggc tgtagtcgtc gcgggaaagc agtattaccg cgccgcctcg
     5641 gcgaacgacc tgcgatggat cctcgcgccg accgcacagc tggtctcctg ggtcaccggc
     5701 cacgagttcg cctacgaggc gggccagggc tggatgaaca ccgatgtcat gttcgtgatc
     5761 gcgccggcgt gcgcaggtgt gaatttcgcg ctggccgcgt ttctcgcact cgtgctcggc
     5821 ggactcgcgg gcatgacatc gttgcctgcc accgcgaagc ggttcggttt cgccgtgctg
     5881 ctcgcgtatg tcgcgacgct cgtcgtcaac acgacacgga tctcgatcgc ggtcgacatg
     5941 caccgcgctg acttgcaccg cctcgaaggc atcgtcgtgt acctcgtcgg cttgtgcggg
     6001 ctctatgcgg ttgcacgcgc gctcgatggc cgcaagttgc gaagcttgaa ctggctcgcg
     6061 attccgctgt gcacgtacgt gatgatcacg ctcgtgatgc cgctcgccaa tggtgcggcg
     6121 caacgtgacg actttgttcg ccacgccacc accgtgctcg cggtgtgcgt cgtgatggtc
     6181 gcgattgcat cgttctgcga gctcgtccgt tggagctgga ggaggaaaca atgaaagcct
     6241 ggctgatccc gttctcgctc gttgtcctcg ccgacaccgc gcacgctgac cagtgcgcgt
     6301 ggatcgacaa gccgaccgcg caaaaagcag aagcgatcgt tctgaaggcg tcgaagtaca
     6361 tcgagttctg cgagccgtgc ggcgagaagg cacctggcat tccgcagaaa gcgcagtcgg
     6421 ttgccgtgca ggatgcggac ggcgactaca aagaggtgac gatcaacggc aagggcgtcg
     6481 acctcgcgta cacgttcgtc aagaccgacg ccggtcacta tcgcaacctc gccgcgctcg
     6541 ccggctgccc ggccgaaggc gtctcgccca gtttggcgat cgaggacgag acgccgaatg
     6601 gcgtgatgat ccgcgcggcg gacgacacgc tgcgtccgca accaccgccg ccgcaaccgc
     6661 tgccggcgcc cgccgctgcg cctccgcagt tgcaggtacc gccgcaggtc tacgtctaca
     6721 acacgacgcg tgacatcgca tggctgccga tcgcgctcgc cgcgtgcggt ggcttcgtca
     6781 ccggctcggc gctcacgctc ctcgtgttcg cagcgcgtcg ccggcgtgcg atgcggccgc
     6841 gtgcggtcga catcgcggcg cgctagctca cttcgggtgg atccgtgcgg ccggtaatgc
     6901 cgccgcatcg gatgccttgc ggtcgatctg gctctgtagc tcgcgcgagt tgagctcgca
     6961 gcgatcgagg agccgttcga gctcgtcgcg cttgcgggtc acgagcaaga cggccatgcc
     7021 cgtcaacttg ggcaacttca gctggtggtg cagcgagcgc aggttgaaca ccgcgtgcac
     7081 catcgcgagc gccatcagcg cgccgccgcc gaacaagagg ccgagcgaga ccgccgtcga
     7141 cgccttcgcg agcgccgcga cgaagtagcc catgccgatg acgatgccga ccgcggcgac
     7201 ggcagcaccg atgcgatgcc gctggcgatt cgcggcaacc cgcgcgtagc agcgctcgca
     7261 catcggcgac acgctcggcg cgagtgtcga actctggatc gagaccgtac cgacgatcga
     7321 gtagcggcgc gcgatctcgt gcgagccata gttcaccagc acgtatgcgg gcgacgtgtc
     7381 ggtcgagtcg caaccgatgc acttacgcga gtgctcgtcg aggcgctgcg tgagctccgc
     7441 ttcgagcacc tcgcgctgct cgcgcagcgc gcgcaccttt tcgagcaacg cgcgccgctc
     7501 gggcgtgtcc tcgatctgcg gcgcgggctt gtcgccggac gcaccgggct cggcggcgga
     7561 gtcgccggag cggagaaagc gcggctcgtc ggtcacgcgc gcatcctatc agcgctcggg
     7621 cggcacgagt tgctgtaact gccaggcctg aaagctgagc ttgtgctcgg cgcggcgcag
     7681 ctcgttgatg aacagccgtc cccagatcac cgcggctccg atcgcaccga acgtcgtgag
     7741 caccatcgcc acgaacgtac ggcccggcgg gaacgcattc atcttcggta acagcacgat
     7801 cgcgccgtcg cgcacctcgt acatcggcga ctgcaagccg aggcgctcga gcacgaacgg
     7861 tgcgacgagc cacagcccga tgaacaccag gatcagcagg ccgtggatcg ccttgtccac
     7921 gttcatgcgc cagccgagcg cgatgatcgc gagcgcggcg ggtacaccac ccagaagacc
     7981 gaacgacgtc atgagcacgg ccgatgcggc gacggtcgcg aactgagccg ggcccgcggt
     8041 gggccgcgtg aggttcgcgc tcacgtactg cagcgcagtc gcgagcagca cgagaccgac
     8101 gtaggcgccg aacaaaggcc agttgcgtac gccaagccac aggatcagcg gcacggccag
     8161 cagcatcatc gccgtcggta gcgtggcgcg ccggagctga cggcgctggc ggtcggtcca
     8221 gcggcggtcc atctcggctt tcgccgcggg cggcaacacc gccggcacgt cggtgagcat
     8281 ccgcatcagc gtgtgcagcg cgagccggtt gtccggatcg aggccgagcg cgcgaccgat
     8341 ctcctggcct gcgatggtgc ggctcgccgg atcgtgatcg gcacgcgcga gtacttctgc
     8401 agcccgccgc gcgtgctcct cgctcacccg cttgcgcgtc tcgagatcgc gatcaccgtc
     8461 gaggaattgc tcgagcgctt cgtggagtgc acgtgccgtc gggtagcggc caccggcatc
     8521 gagcgccgat gcgcgcatct ggatcgtctc gagctcgggc ggcacgtcgt gctcgctgtg
     8581 ggcggcgagg atctcaccga ggatcacgcc gagcgcgaac acgtcggtct gcggattcgc
     8641 gacaccgagc tggcgctcgg gcgacatgta cttcggcgtg ccgagcgtcg agccggcctt
     8701 cgttgcattc gtgccgatgc cgacgtcgat cgtgtcggcg agcggcgtct cgccgcggtg
     8761 catgaccttc gcgatgcccc agtcgagcac gtagacctca ccgaactggc cgagcatcac
     8821 gttctcgggc ttgagatcgc gatgcacgac tccgcgcgcg tgcgcgaagt cgaccgcgag
     8881 gcacacgctc gagaacgcgg tgagcaaccg gcgccgcgag tactgcgggg tgcgcacacc
     8941 ggccgccagc gaatcgatcg cctgacgcag ggtccggccg cgcacgcact tcatcgcgaa
     9001 gtacagctcg ccgccggcgg tctcgccgag atcgtgcacc ggcacgatcg ccggatgctc
     9061 gagctgtccc tgcacacgcg cttcgagcag gaaccgcgcg cggtaacccg gatcgttctc
     9121 gacgccggca cgcagcactt tgtacgcgac gtagcgcgag atccgccggt cgcgcatcag
     9181 gcgcacttcg cccatcccgc ccttaccgag taggccgatc gaatcgaacc gttcggcgtc
     9241 ggacacgagc ggcgtgtcct cgacatcgac gggctgctcg tcgtcgttga ccttggtgag
     9301 gttctccccg tccagcgcgt gcgtgtccgt catcacgcac catgctaacg ccgtcgcact
     9361 actgcggggg cgcgactggc acgtcccaca agcgaacgtt gtcgaccgcg ccgacgagcc
     9421 actcgtcagc cccggtcggg ccaccgttgt tgttctggcc gatcgtcatg ccggcggaaa
     9481 caaacccgcc gccgctcgag cagcccgacg tggcctgctc gccgatcttc gcgccgtcga
     9541 ggtagatctg cagcgtatcg gcagtgcacg tgcaaaccgc gtcgtgccac atgccgagca
     9601 cgaggggcgc gtcgaatgcg tagagcgcgc caccgagccc gcaacgcagc tgatagcccg
     9661 gatcgactcg atacaagaag aagctgacgt tcggcgggat gacgttggtg tcgaggatgc
     9721 cgagccgcgc gccgttgatc gccggtgccc cgtcagcgcg aaacgagacc tcgaacccct
     9781 ggatgttcgt gacctcggcc gtgtatggca cgtagatcat gctcgtcgaa tcgatctgag
     9841 ccgcgccggt cgcacctcgc gccgtatgcg tgacgttcgt gagcgtcgca ctgacgtttg
     9901 ccgcgccctc gttcgcgagt gagggcggca gcggatcctg atcgaacgag tagcagagcc
     9961 gcaggtgcgg atcggccgcg cagaacggcg ctggcgcgtc gatcatcatg tcgacgttcg
    10021 cgtcgggcgc gtcggggatc gccgcgtcct cggtcgccgt gccgtgctga aagccgcaac
    10081 cggcgagcac gagtacgacg tacgaccgca cctgtcgatc atagtccgcg agcgggcgaa
    10141 agggtgcgtg atcgatgtag cctgcgctcg tgcgcacggt ccgcgcgact cgctacttga
    10201 cgccgctccg cgaaggtggc tccgtgcccg cgatcatcga ggccgacgac gacggcacgt
    10261 atgtcgcgaa gtttcgcggc gccgcgcagg gtgaaaaggc gctcatcgcc gagatcatct
    10321 gcggcgagct cgcccgcctg ctcggtctgc cggtgcctca gatcgtgcac gtcgatatcg
    10381 acctgttgct gtcgcgctcc gagcccgacc aggagctgca ggagctactg cgcaagagcg
    10441 ccggtacgaa cgtcgcgctc gactacctgc caggcgcgct caactgggag ccgccgctcg
    10501 cgccgccacc ggatcgtgac cttgccgcgc agatcgtgtg gttcgacgcg ctgatcacga
    10561 acgtcgaccg cacggtgaag aacccgaaca tgctgatctg gcaccgccgc ctgcatctca
    10621 tcgatcacgg cgcggcgctc tactttcatc atcagtggga cgatcacctg gcgcgcgcga
    10681 agggcgcgtt ccctgcgatc cgcacgcatg ccctgttgcc gcttgccggc gatctcgccg
    10741 ccgccgacgc cgcgctcgct ccgaagatcg acgacgccgg tttgcgtgcg attctcgcgg
    10801 acgtgcccga cgag
//
LOCUS       DASUFX010000141        17071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_717320,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000141 DASUFX010000000
VERSION     DASUFX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717320"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFV99_05595"
     CDS             complement(<1..567)
                     /locus_tag="VFV99_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5058812.1"
                     /translation="MRILVGTALGAIIGYERDIHGRPAGLRTHAIVALASATFMVVST
                     HLAYYQGYREGDFTEVDGSRIAASVVSGIGFLAGGAILRTGLTIKGLTTAAGLWLVAA
                     IGLCAGGGMYLESIAATVVGVATLTILRRFEDKDDRVRRRLTLTLTVEALATDALVDK
                     LTKLDLRPTVEGWNLEHAASLRQVTLGVR"
     gene            complement(686..1966)
                     /locus_tag="VFV99_05600"
     CDS             complement(686..1966)
                     /locus_tag="VFV99_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5690 family protein"
                     /protein_id="HEX5058813.1"
                     /translation="MSQSGEHRVTRWLAKASPGLFATYAVAMAFTTYFAMYSYRKPFA
                     AAHFAGQSFWILDLKSALIVSQLFGYALSKFLGIKFNSEMPPQRRAWALVFLIVWAEV
                     ALVLFAVLPPQGKVVALFLNGLPLGTVWGVVFSFLEGRQTSEILGAGLSCAYVVASGA
                     VKSIGATLLGHGISEAWMPALTGVMFLPIFFAAVYGLSLIPAPSPADIAARTHREPMN
                     AVERRAFVRRFVPGLLLLVIVYLFLTSYRDFRDNYAAEIWADLGHDQQAGVFTLTEIP
                     IAMSVMVVLGLLYLVKNNKRGLLLTYVIMGTGALMVGASTLLFDGGAIGPQAWMILVG
                     LGLYLGYVPYGCVLFDRMIAALGIVATAVFLIYVSDAVAYGGSVGIVLYKVLGQADIS
                     KLQFFRYFSYAMSAVCMLFLAVSGTYFLRRARST"
     gene            2067..2873
                     /locus_tag="VFV99_05605"
     CDS             2067..2873
                     /locus_tag="VFV99_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058814.1"
                     /translation="MVTVDAIIERGRAAHPQVTVARDVIAPFVTLRLDGEERPDELDA
                     EEVFLASACAAGDRTAIAAFERRYFGVIPQALSRLSLGRDEIREIEQVLRVRLFVAAD
                     GDSVPRVVSYAGRGQLGGLLRVAAIRAGLNLLRDRGRLDASAADDLEDVPIANDDPEL
                     AKLKAQHRTAFKAAFEEAISTLVPRERSLLDLAIVKRVGIDRIGAIYGVHRATAARWV
                     VTAQNNLARAVHRILGARLGVREAELENLLPLVESQLELSLERLLKSRAG"
     gene            complement(2870..5773)
                     /locus_tag="VFV99_05610"
     CDS             complement(2870..5773)
                     /locus_tag="VFV99_05610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058815.1"
                     /translation="MVCPDERVMLDLVAGRIPAQAIALRDHLDSCDSCRDVVAALSSS
                     VEPTPASVQPGSRIGRYVIVDELGRGAMATVYRARDPELDREVALKLVHFRDGGTSAE
                     RLLREARAMAKLTHPNVTRIYDVGTEGDAVFLAMELVAGMDLARWLRDSHTWREVVAV
                     FAAAADGLAAAHRQLLIHRDFKPANVLVGDDGRVLVSDFGLVHTPSEADRELAAGQVA
                     DDVLLTRTGAIVGTPAYMAPELLATAAAADELSDQFSFAVALFEGLYGVRPFVGDDLQ
                     ALRANVLAGRIRVLPADRDVPGWLHQLVLRGLGPRERRFASMAALAGALRADPRRRRR
                     LIGAFAGVALVAATAVAGASKLAAKEAVDPCAAGDDKIAASWSPLKAIELRVGFAKSG
                     VPFAADAYTATAARLDRFRTDWAAGYRAACQASRAGVQSGDLLDRRMQCLDSRRHELD
                     ALLGVLAAPDRSTIAHAPETADALSRVADCGNTVALGTLMPPPAGVDAGAIDKVKAGI
                     ARVHVLRNSPNPKHVIEEADQVLADAKKLGYAPVLAEANVASAIVHMVANQTKEANQA
                     VEDAVVAAEAGRDFYTKAHALAVGMELAYNTGDFTGAVNRDRLARAALSAIGGNEKLE
                     ADLESVLGWIAARQEDESAAEKHLRRALELRIHALGAQDLTTARAYENLGTALGNRDL
                     AAALGEYEKGLAIERAQLGGKHPAVAQTLGNVAIIQSSLGKQTEAIATAKEALAILEG
                     SMPVNHPAVAFATLQLAGVYHQAHDYEHALSYQRKAVERYEAAVGPDHADLGRALTNL
                     AATLAELERYDEMVPVARRALAIQEKAFGANSPGTATIRNNLATALAGVGKNDEALEQ
                     FQLALALREKAYGKDDPRLLSTLLGISAVHQARKAWREQIAILERAMTFEPKAKAPRD
                     KSLIAKAAFELAMVQRAHGNKQRAKPLAERALAMYRELDRADDAAAVEKFLRG"
     gene            complement(5877..6500)
                     /locus_tag="VFV99_05615"
     CDS             complement(5877..6500)
                     /locus_tag="VFV99_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5058816.1"
                     /translation="MIDDVLAFWFGEPATTTEEYGRKIRRWYMGGPALDAEIRDRFGA
                     LVERALAGELADWTQTTRGRLALILLLDQFTRAMYRNDPKTYAGDARAQALAVEAFDR
                     GIDRELSVEERQFLIMPLTHAENLALQERSAATMAALNADAANFQKGPLSMGLEQTRK
                     YRDLIARFGRFPHRNKVLGRTNTPEEDAFLVDWEAKMRPSGADKLPS"
     gene            complement(6497..8416)
                     /locus_tag="VFV99_05620"
     CDS             complement(6497..8416)
                     /locus_tag="VFV99_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058817.1"
                     /translation="MSALESTLVHLPSLDSATLWGAIESAAAEARFGIFIVKIDVQPP
                     HIVYVNERAAEIVGRSRDEMIGQEPSIFLRPQDRPMVPASIARPTGAGAPPTSHELVI
                     QRPDGQQIPISLASTRMQTAIGMLSFGYFRDVSRERDAVEALRSSEARFRLLVEAAPD
                     GVVILRRGDIAFINPRATQMLGLEKGEDPIGRSIASFLMPADAALATQRITAMLRDGV
                     EFPPSEYSLRESARVVEIKSIVCEWEGGPGVLAFARDVTERRAIQRRLVESDRLAALG
                     TLAAGVAHEINNPLTYARLSAQRIERIIDTLDVPTAALATLHGHLGDINHAISRIASI
                     TQSLRTFVSRDDDAPGPVDMSMVLARSLKMVDNELRHVARLVYTPASLPPVTGNASRL
                     EQVFVNVLINAIKALPPNPPTPHEIRVAVEHSGARVTIAITDTGIGIPPALLSRIFDP
                     FFTTRDVGHGMGLGLSVSKTIIEKLGGEIAVESTENVGTTVRVHLPTHRASSPVVAAA
                     VATAVPNLPRLRVLVVDDEPMICASLERMLRKEHDVSVAASGPEALGAVATTPFDVIL
                     CDVMMPGMNGDEVFHTLANRHPGFERRIVFMSGGTIGSDVLRLLDGLPNERIAKPFTV
                     DQVLGVIAQAYGGSK"
     gene            complement(8483..9625)
                     /locus_tag="VFV99_05625"
     CDS             complement(8483..9625)
                     /locus_tag="VFV99_05625"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5058818.1"
                     /translation="MTIVRASGIALVALLAGCGDDSNNNNKTPDAFIIHDMGIDSRAC
                     ESVTPGMLDFNGFDPQGFVSWKGPITATGLGTNLEYQFEFYDNIESSLMGTFDLKAGN
                     QSNYSTCAICVRAFERDAQGQVVKQYYQSGGSITLTEDPFTNKRLVGSLTDLQLEEVT
                     IAQQTFVSTPVPGGKCGNFGGYAVDHDRVPNAWTCTHTDYDSGTNCNCVCGTPDPDCI
                     INNATVVGCTTGQACFNDACVAKPANDTCAQAVTLTLGTSVMGTTAGADRNYNAGLEG
                     SACTGFGQPGPDVVYKIDLTANQAITVTLSGLAATYDGSIALVGPGADTLCDANPIAT
                     CVAGADDKFDGQDETFTYTATTAGTYYVIVDAYAPNVGGTFTLLVN"
     gene            9698..10096
                     /locus_tag="VFV99_05630"
     CDS             9698..10096
                     /locus_tag="VFV99_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058819.1"
                     /translation="MSKLAALGVTVKRQYVTVDGAEMTDLVVTLPKSTGVEATFSAED
                     FADKVFKVFKREIQVGDPIFDEAVHIKTDTTDQTEKLLQSSDMRAIIERVIVNGGAIE
                     IDGNTVTMKVAGRQEKDDEVFELFVTTLLG"
     gene            complement(10113..11471)
                     /locus_tag="VFV99_05635"
     CDS             complement(10113..11471)
                     /locus_tag="VFV99_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058820.1"
                     /translation="MRSTRSATLRHILTAGLVLFATRGAVAQPAPTDPPPATPPADQE
                     QPPATPTAPAAEPPVPVEETPLKPEPEKKTEEKRAVTIKYDNGMKFSTADDQFELKLQ
                     FRNQVRFEANRPLDDETPTRHNQWFNRFYIPRARLQAEGHLFGNDNRFKAELGMGDSG
                     SFAFLRDMYLEKRLPDSPIYLRFGQWKRPYNRMEIVSDFSSTFNERSIENEQAGGGRD
                     LGIALHNDYEKSPAPIEWAVGMFNTLNGGQDRPTWTTACVQDPVTLKIACTNTRPTTF
                     PLDFEPALVARVGYNTPNIKGYSESDLEGGPLRYAVAASYKVGLGNLNKGAQDSWADN
                     LSHGFEIDTMIKAWGFGLEAGVELQKLKTADANVGFYVQPSYFLTPKTIEVAGRFALI
                     TVKEIADDRKEIETRVAFNYYWHGHIWKIASDGGFIQFLGDTPAPDQPDLQFRVMMQM
                     QI"
     gene            11612..12688
                     /gene="pstS"
                     /locus_tag="VFV99_05640"
     CDS             11612..12688
                     /gene="pstS"
                     /locus_tag="VFV99_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX5058821.1"
                     /translation="MKMWLASVVSIGLIASCGKKREDGGGSTGTAGTASGGGAASARK
                     ITINGSGSTFQKAYQEIAIETYANAHGEVTVNYGGGGSGKGRQDFADMVTDFGCTDAP
                     FKDAEKSKIKGGELVYVPVLLGAISVSYNVPEIEKLQLSAETLAKIFQREIKKWNDPA
                     IAQDNPGAKLPATDIVVAHRSDGSGTTANFTLFLTKAAGAAWKLKSGSTVEWSADTQA
                     GNGNSGVAQIVKSTHGAIGYVDLPDAKASGLKYAAIKNQAGKYVEPTPESTQAAGEGI
                     EVKDDLTFEAVNAKGDAAYPITAQSWCMAYTKPADQAKGGAVKAYYKYLVTDGQGLLK
                     EIDFAPLPKTLQDKAIAQVEKIQI"
     gene            12688..13641
                     /gene="pstC"
                     /locus_tag="VFV99_05645"
     CDS             12688..13641
                     /gene="pstC"
                     /locus_tag="VFV99_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5058822.1"
                     /translation="MADKIDLRGTPSRADQAFRGLAMGAACLVLLVLALIAIQMSGRM
                     GPVFDRMGFSYFTSKRWSPAEGLFGALPFLWGTLYTACIAIVLSVPVSLFVALFVTQV
                     ASPRWRKPIVYVLDLLAVVPSVVYGLWGVLVLAQPLQKIFLKIHNAVDGIPGLQSIFG
                     PTPQGRSFMTAGVILAIMITPIITSLAREVIETTPQNEKEAAYALGATRWEMIRASVW
                     PHSKSGLVGAVMLGLGRAMGETIAAALVIGSSIGQITINAFAPGNSMPAVIANEWGEA
                     NDLRKSAMIALALLLFAITIIVNLVSSAIVQRSLRRSRGAL"
     gene            13638..14534
                     /gene="pstA"
                     /locus_tag="VFV99_05650"
     CDS             13638..14534
                     /gene="pstA"
                     /locus_tag="VFV99_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5058823.1"
                     /translation="MRHPGDPVVAISLEPRLSWRTIQNWLMTGLMIAAFVAITIPLIA
                     ILYSLVSRGAAIALRGFPDFFTATIPIVSRRAGPGMGPAIVGTLICTGGATIIAVPLG
                     ILGAVYLHEYGKQSKFARLVRFMAMVMTGVPSIVMGLFVYVLWTMRFGYSAFGGGLAL
                     ACLMLPIIIGATEQMLRLVPDHLREAAFALGASKSRVILTVALPAALPGIVSGCLLAI
                     ARAAGETAPLLFTIGAATKFNPHIFDNANTALSVQIFGNATSSFAAAQERAWGAALTL
                     VVLTFLLTLVARIVAARFAAKR"
     gene            14555..15427
                     /gene="pstB"
                     /locus_tag="VFV99_05655"
     CDS             14555..15427
                     /gene="pstB"
                     /locus_tag="VFV99_05655"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5058824.1"
                     /translation="MQTIIIEPAHPSRPSYRVHVDDPMPAVEDAPVVFALDKLEVFYG
                     AFRAVRDVSLDIHKNEITAFIGPSGCGKTTVLRCLNRMNDLVPGARIGGHITYHGIDL
                     YGSDVSAAEVRRRIGMVFQKPNPFPKSIYENIAFGPRINGIRDRGKLDEVVEKSLRAA
                     ALWDEVKDRLHSSALAMSGGQQQRLCIARTIAVEPEVVLMDEPCSALDPIATARIEEL
                     MRQLKRTYTIVIVTHNMQQAARVSDRTAFFTAEVDPKAERRTGVLVEYDVTSRIFSNP
                     REERTENYITGRFG"
     gene            complement(15437..16696)
                     /locus_tag="VFV99_05660"
     CDS             complement(15437..16696)
                     /locus_tag="VFV99_05660"
                     /inference="COORDINATES: protein motif:HMM:NF002461.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX5058825.1"
                     /translation="MHKKPFFTTLYFRVLVAIGLGVLVGVLWPSTAAKLAPLGEGFIK
                     LIKLTIAPLIFCTIVGGIASMKSMKSVGKAGGLALLYFEVASTLALAIGLVIVNFVQP
                     GVGMNIDPSKIDPASIAKYAGPGKMKSTVDFFLDVIPDTFVGAFVHGELLQVLLVAIL
                     FGFALHAIGERGKALFDLVEELGQVIFKIVGLIMHLAPIGAFGAMAFTVGANGLHSLV
                     ALGELMACFYGTCLIFIFGVLGTVARVHGFSILAFLRYIREELFIVLGTSSSESVLPR
                     MIDKLERLGAGPSTVGLVVPAGYSFNLDGTSIYLTMGAMFVAQATNTHLDVTDQLALL
                     AVLLLTSKGAAGVTGSGLVVLAATLKAVGDVPVAGIAVIVGIDRFMSEARALTNVIGN
                     GVATLVVAKWVGDLNKPKLDEELASGP"
     gene            complement(16698..>17071)
                     /locus_tag="VFV99_05665"
     CDS             complement(16698..>17071)
                     /locus_tag="VFV99_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058826.1"
                     /translation="RDYDLHYMANANPTLEPFALADVHADSDVTLTAAWPPEAVETYL
                     YYDALTQRLVDRREAMRVSWFAMGGELAVDASAVDEDGTATSVSTTWHTPAAGDAWLW
                     LVLRDSRGGLAWQELHVTVTP"
BASE COUNT         2993 a         5561 c         5546 g         2971 t
ORIGIN      
        1 ccgaacgcca agtgtcacct gtcggagtga cgccgcgtgc tccaagttcc agccttccac
       61 cgtgggacgc aggtcgagct tggtgagctt gtcgacgagg gcgtccgttg cgagcgcttc
      121 taccgtgagc gtcaacgtca gccgccggcg aacgcgatcg tccttgtcct cgaaccgtcg
      181 caagatcgtc agtgtcgcga cgcccacaac ggtcgccgcg atgctctcga gatacatacc
      241 gccgcccgca cacagcccga tggcggccac gagccaaagg cccgctgccg tcgtgagtcc
      301 tttgatcgtg agtcctgtgc gaaggatcgc accaccagcc agaaagccga tgccggatac
      361 gaccgaggcg gcgattcgag atccgtccac ctcggtgaag tcgccctcgc ggtacccttg
      421 gtagtatgcg aggtgtgtcg agacgaccat gaacgtcgcc gacgccaagg cgacgatcgc
      481 gtgggttctc aaacccgctg gtcgcccgtg aatgtcgcgc tcgtatccga tgattgcgcc
      541 gagcgcggtt ccgacgagga ttctcacgat cagctcgaca tcctggttca actctcgacc
      601 tccgttgcct tgaagtgcat cgatcgatgc tctgcctacg caagaatttg tcaaccaaga
      661 ttcacacttc gacacagtcg agaaatcaag tcgaacgcgc tcgacggagg aagtacgtac
      721 cgctgaccgc gaggaacaac atgcacacgg cgctcatcgc gtacgagaaa tagcgaaaga
      781 actgcagctt cgaaatgtcg gcctggccga gcaccttgta gagcacgatc ccgaccgagc
      841 cgccgtacgc gacggcatcg ctcacgtaga tcagaaatac cgccgtcgcg acgatgccga
      901 gcgccgcgat cattcgatcg aacagcacgc atccgtacgg tacgtagccg aggtacaacc
      961 caaggccgac gaggatcatc caggcctgcg gcccgatggc gccaccgtcg aacagcagcg
     1021 tcgacgcgcc gaccatcagc gcgccggttc ccatgatcac gtaggtgagc aacaacccgc
     1081 gcttgttgtt cttcaccagg tagagaagac cgagcacgac catcacggac atcgcgatcg
     1141 gaatctcggt gagcgtgaag acgccggctt gctgatcgtg accgaggtcc gcccagatct
     1201 ccgcagcata gttgtcgcga aaatcgcggt acgacgtcag gaacaaatag acgatcacga
     1261 gcagcaacag gccgggtacg aatcgcctca cgaacgcgcg ccgctcgacc gcattcatcg
     1321 gttcgcgatg cgtacgcgcc gcgatgtcgg cgggactcgg cgccgggatc aagctcagcc
     1381 cgtacaccgc cgcgaagaag atcggcagga acatcactcc ggtgagcgca ggcatccacg
     1441 cttcgctgat gccgtggccg agcagcgtcg cgccgatcga tttcacggcg ccgctcgcga
     1501 cgacgtacgc acagctcagg ccggcgccga ggatctcgga ggtctgacga ccctcgagaa
     1561 agctgaacac gacgccccac acggtgccga gcggcagtcc gttcaggaac aacgcaacga
     1621 cttttccctg cggcggcaac acggcgaaca gcaccagcgc cacttccgcc cacacgatca
     1681 ggaacacgag cgcccacgcg cgtcgctgcg gcggcatctc cgagttgaac ttgatcccca
     1741 gaaatttcga gagcgcatag ccgaacagct ggctgacgat gagcgcgctc ttcagatcga
     1801 ggatccagaa gctctggcct gcgaaatgcg cagccgcgaa cggcttccga tagctgtaca
     1861 tcgcgaagta ggtcgtgaat gccatcgcga ccgcgtacgt cgcgaacagt ccgggagatg
     1921 ccttcgctag ccaccgcgtg actcgatgct caccagactg actcatgggt cgtcaccgta
     1981 acccggtttg tgtccttggc ctcacgagga gcgccgtgtg tccgctacaa aacacacgta
     2041 ggtgacacct gtgatatgag cgcgccatgg tgacggtgga cgcgatcatc gagcgaggcc
     2101 gagcggcgca tccgcaggtc acagtcgcgc gagacgtgat cgcgccgttc gtgaccctgc
     2161 gcctggacgg cgaggagcgc cccgacgagc tcgacgccga ggaagtgttt ctcgcgtccg
     2221 catgcgccgc aggtgaccgc actgcaatcg cggcgttcga gcgccgctac ttcggcgtga
     2281 tcccgcaggc gctgtcgcga ctctcgctcg gccgcgacga gatccgcgag atcgagcagg
     2341 tcctgcgcgt gcggctgttc gtcgccgccg atggcgattc ggtaccgcgc gtcgtcagct
     2401 acgccgggcg cgggcagctc ggcggcctgt tgcgggtggc cgcaattcgc gccgggctca
     2461 acttgctgcg cgatcggggt cgcctcgatg cgagcgccgc cgatgacctc gaagacgtgc
     2521 cgatcgcgaa cgacgacccc gagctcgcaa agctcaaggc gcagcatcgc actgcgttca
     2581 aagccgcgtt cgaggaggcg atcagtacgc tcgtgccgcg cgagcgcagc ttgctcgatc
     2641 tcgcgattgt caaacgcgtc ggcatcgatc gcatcggcgc gatttacggc gttcatcgcg
     2701 cgacggccgc tcgctgggtg gttactgcac agaacaacct cgcgcgggcc gtgcaccgga
     2761 tcctcggtgc gcgactcggg gtccgcgagg ccgagctcga gaacttgttg ccgctcgtcg
     2821 agtcgcagct cgagctatcg ctggagcggc tgctcaagtc acgcgccggt tagccgcgca
     2881 ggaacttctc gacggccgca gcatcgtctg cgcggtcgag ctcgcggtac atcgcgagtg
     2941 cgcgctcggc gagcggcttc gcgcgctgct tgttgccgtg cgcacgctgc accattgcga
     3001 gctcgaacgc ggctttcgcg atcagcgatt tgtcccgcgg cgcctttgcc ttgggctcga
     3061 acgtcatcgc acgctcgagg atagcgatct gctcgcgcca tgccttgcgc gcctgatgca
     3121 ccgcggagat tccgagaagc gtcgacagca ggcgcggatc gtccttgccg tacgccttct
     3181 cgcggagcgc gagcgcgagc tggaactgct cgagtgcctc gtcgttctta ccgaccccgg
     3241 ccagcgcggt cgcgaggttg ttgcggatcg tcgcggtgcc cggcgaattc gcgccgaatg
     3301 ctttttcctg aatcgcgagg gctcggcgcg cgaccggcac catctcgtcg tagcgctcca
     3361 gctccgcgag cgtcgcagcg aggttcgtga gcgcgcgacc gaggtcggcg tggtcgggac
     3421 cgacggctgc ctcgtagcgt tcgacggcct tgcgctgata actcagcgcg tgctcgtagt
     3481 cgtgtgcctg gtgatagaca ccggccagct gcagcgtcgc gaacgcgacc gccgggtgat
     3541 tgaccggcat gctgccctcg aggatcgcga gcgcctcctt cgccgtcgcg atcgcctcgg
     3601 tttgcttgcc gagcgacgac tggatgatcg cgacattgcc gagcgtctgc gcgacagcgg
     3661 ggtgcttgcc gccgagctga gcgcgctcga tcgccaaacc tttttcgtat tcgccgagcg
     3721 ctgcggcgag gtcgcgattg ccgagagcgg tgccgaggtt ctcgtacgcg cgcgcggtcg
     3781 tcagatcctg cgcgccgaga gcgtggatgc gcagctcgag ggcacggcgc aggtgcttct
     3841 cggcagcaga ctcgtcctcc tgacgcgcgg cgatccagcc gagcacgctc tcgaggtcgg
     3901 cctccagctt ctcgttgcca ccgatcgccg atagcgcagc gcgagcgagc cggtcgcggt
     3961 ttaccgcgcc ggtgaagtcg ccggtgttgt aggcgagctc catgccgacg gcgagtgcgt
     4021 gcgccttcgt gtagaagtcg cgacctgcct cggcggcgac gactgcgtct tcgaccgcct
     4081 ggttcgcttc cttcgtctgg ttcgcgacca tgtgcacgat cgccgacgct acgttcgctt
     4141 cggcgagcac cggcgcgtag ccgagcttct tcgcgtcggc gagcacctgg tcggcttcct
     4201 cgatcacgtg cttcggattc ggcgaattgc ggagcacgtg cacgcgcgcg atgcctgcct
     4261 tcaccttgtc gatcgcgccg gcatcgacac cggccggcgg cggcatcagc gtgccgagtg
     4321 cgacggtgtt cccgcagtcg gcgacgcggc tcagcgcatc tgccgtctcc ggtgcgtgtg
     4381 cgatcgtcga acgatctggc gccgccagca cgccgagcaa cgcgtcgagc tcgtggcgcc
     4441 gcgagtcgag gcactgcatg cggcgatcga gcaggtcgcc cgactgcacc cctgccctgc
     4501 tcgcctggca ggccgcgcga taacccgctg cccagtcggt gcggaatcga tcgagccgcg
     4561 ccgcagtcgc ggtgtacgca tctgcggcga acggcacacc gctcttcgca aacccgacgc
     4621 gcagctcgat cgccttcagt ggcgaccacg atgcggcgat cttgtcgtcg ccggcggcgc
     4681 acggatcgac cgcttccttc gcggcgagct tgctcgcccc agcgactgca gttgcagcga
     4741 cgagcgcgac acccgcaaac gcgccgatca agcgacggcg gcggcgagga tccgcgcgca
     4801 gcgcacctgc caacgccgcc atcgacgcga agcgacgctc gcgtggcccg agcccgcgca
     4861 gcacgagctg atgtagccag cccggcacgt cgcgatcggc cggcaagacg cgaattcgac
     4921 cagcgagcac gttggcgcgc agcgcttgca gatcgtcgcc gacgaacgga cgcacgccgt
     4981 agagtccttc gaacagggcg accgcgaagc tgaactgatc cgacagctcg tcggcagcgg
     5041 ccgcggtcgc gagcagctcg ggcgccatgt acgccggcgt gccgacgatc gctccggtcc
     5101 gcgtcagcag aacatcgtcg gctacctggc cagctgcgag ctcgcgatcg gcttcgctcg
     5161 gcgtgtgaac gaggccgaag tcgctaacga gcacgcgacc gtcgtcgcca acgagcacgt
     5221 tcgcgggctt gaagtcgcgg tggatcagca gctggcgatg cgccgctgcc agaccgtccg
     5281 ccgcggcggc aaacacggca acgacctcgc gccacgtgtg gctgtcgcgc agccagcgcg
     5341 cgagatccat gcctgcgacc agctccatcg cgagaaacac ggcgtcgccc tcggtgccga
     5401 cgtcgtagat gcgcgtcacg ttcgggtgcg tgagctttgc catcgcgcgc gcttcgcgca
     5461 gcaagcgttc agcgctggtg ccgccgtcgc ggaaatggac gagcttgagc gcgacctcgc
     5521 gatcgagctc cggatcgcgt gcgcgatata cggttgccat cgcaccgcgg ccgagctcgt
     5581 cgacgatcac gtagcgaccg atccgactgc ccggctgtac gctcgcgggc gtaggctcga
     5641 cgctgctgct gagtgccgcg accacgtcgc gacacgaatc gcagctgtcg agatgatcgc
     5701 gaagcgcgat cgcctgcgcg gggatccggc cagcgacgag gtcgagcatc acgcgttcgt
     5761 cggggcagac catgcggccg ccgtgtgtaa gccaatcgcg accccgccgt cgagcacgat
     5821 cttgagtcgg gccagttacg tcatgtacgc gcggacttgt ggcgtacctg acgctgctac
     5881 gacggcagct tgtcggcgcc gctcggcctc atcttcgcct cccagtcgac gaggaaggca
     5941 tcctcttcgg gcgtgttcgt ccggccgaga accttgttgc gatgcggaaa ccgcccgaag
     6001 cgcgcgatga gatcacggta cttgcgcgtt tgctcgaggc ccatcgagag cggccccttc
     6061 tggaagttcg cggcatctgc gttcagtgcc gccatcgtag cggcgctgcg ctcttgcaac
     6121 gcgaggttct ccgcgtgcgt gagcggcatg atcaagaact gacgctcttc gacggacagc
     6181 tcgcgatcga tgccgcgatc gaacgcctcg acggcgagcg cctgcgcccg cgcgtcaccg
     6241 gcgtatgtct tcggatcgtt gcgatacatc gcgcgggtga actgatcgag cagcaggatc
     6301 agcgcgaggc ggccgcgtgt ggtctgcgtc cagtcggcca gctcgccggc gagcgcacgc
     6361 tcgaccagcg caccgaaccg atcacgaatc tcggcatcga gcgccggtcc gcccatgtac
     6421 cagcgccgga tcttgcgccc gtactcttcg gttgtcgtgg ccggctcgcc gaaccagaac
     6481 gccagcacat cgtcgatcat ttgctccctc catacgcctg cgcgatcacg ccgagcacct
     6541 ggtcgaccgt gaacggcttt gcgatccgct cgttcggcaa cccgtcgagt agcctgagca
     6601 cgtccgatcc gatcgtgccg ccgctcatga acacgatgcg gcgttcgaag ccgggatgtc
     6661 gattggcgag cgtgtgaaag acctcgtcgc cattcatccc gggcatcatg acatcgcaca
     6721 ggatcacgtc gaacggcgtg gtcgcgacgg cacctagcgc ttcaggacca ctcgccgcga
     6781 cgctgacgtc gtgctccttg cgcagcatgc gctcgagcga tgcacagatc atcggctcgt
     6841 cgtcgacgac cagcacgcga aggcgtggca ggttcggcac ggccgtcgcc accgccgcag
     6901 cgacgactgg cgagctcgca cgatgggtcg gcagatgaac ccgcacggtc gtgccgacgt
     6961 tctcggtcga ctccaccgcg atctcaccgc cgagcttctc gatgatcgtc ttcgacaccg
     7021 agagcccgag ccccatgccg tggccgacgt cacgcgtcgt gaagaacgga tcgaagatac
     7081 ggctcagcaa cgcgggcgga atgccgatgc cggtgtccgt gatcgcaatc gtcacacgcg
     7141 caccgctgtg ctcgaccgca acacgaatct cgtgcggtgt tggcgggttc ggtgggagcg
     7201 ctttgatcgc gttgatcagg acgttgacga agacctgctc gagtcgcgat gcgttcccgg
     7261 taaccggcgg caacgacgcc ggcgtataga cgagccgcgc cacgtgccgg agctcgttgt
     7321 cgaccatctt gagcgagcgc gcgagcacca tggacatgtc gactggtccc ggcgcgtcgt
     7381 cgtcgcggct gacgaacgtg cgcagtgatt gtgtgatcga cgcgattcgc gagatggcgt
     7441 ggttgatgtc gccgagatgg ccgtgcaggg ttgccagcgc cgcggtcggg acatcgagcg
     7501 tgtcgatgat ccgctcgatg cgctgcgcgc tcagtcgtgc gtacgtgagc ggattattga
     7561 tctcgtgcgc gacgccggcc gcgagcgtac cgagcgccgc aagccgatcg ctctcgacga
     7621 ggcgacgctg gatcgctctg cgttcggtca cgtcgcgcgc gaacgccagc acacccggcc
     7681 cgccttccca ctcgcacacg atcgacttga tctcgacgac acgcgccgac tctcgcagag
     7741 agtattcgct cggcgggaac tcgacgccgt cgcgaagcat cgcggtaatc cgttgcgtcg
     7801 caagcgcagc atccgcaggc atcagaaacg acgcgatcga gcggccgatc gggtcctcgc
     7861 ccttctcgag gccgagcatc tgcgtcgcgc gcgggttgat aaacgcgatg tcgccgcgcc
     7921 gcagaatcac gacgccgtcg ggagccgcct cgacgagcaa tcgaaaccgc gcctccgagc
     7981 tccgcaacgc ttcgaccgca tcgcgctcgc ggctgacgtc acggaagtaa ccaaagctca
     8041 gcatcccgat cgcggtctgc atccgcgtcg acgcgagcga gatcgggatt tgctgcccgt
     8101 cgggacgctg gatgacgagc tcgtgcgacg tcggaggagc ccccgccccg gtcggacgtg
     8161 cgatcgacgc cgggaccatc ggtcgatctt gcgggcgcag gaagatcgac ggctcctggc
     8221 cgatcatctc gtccctgctg cggcccacga tctccgcggc ccgctcgttc acgtagacga
     8281 tgtgcggcgg ctggacatcg atcttgacga tgaagatgcc gaagcgagct tctgccgcag
     8341 cactctcgat cgcaccccac aatgtggcgc tatcgagaga gggcaaatgg acgagcgtcg
     8401 actcgagcgc cgacatctca accattggac ctgggaatca ccgccgaggg aacggccccg
     8461 gccgtggatt ttccgcttcc ggtcagttca caaggagcgt aaacgtgccg cccacgttgg
     8521 gggcatacgc gtcgacgatg acgtagtacg taccggcggt cgtggccgtg taggtgaacg
     8581 tctcgtcctg cccgtcgaac ttgtcgtctg cacccgcgac gcaggtcgcg atcgggttgg
     8641 cgtcgcagag cgtgtcggct cccggaccga cgagcgcgat gctgccgtcg taggtcgccg
     8701 cgaggcccga cagcgtgacc gtgatcgcct ggttcgcagt gaggtcgatc ttgtagacga
     8761 cgtccgggcc gggttgcccg aagccggtgc acgccgagcc ctcgagaccc gcgttgtaat
     8821 tgcggtcggc gccggcggtc gtgcccatga ccgaggtgcc gagcgtcagc gtgaccgcct
     8881 gtgcacaggt gtcgttcgcc ggcttcgcca cacacgcgtc gttgaagcat gcctgaccgg
     8941 tcgtgcagcc aaccaccgtg gcgttgttga tgatgcaatc gggatccggc gtaccgcaca
     9001 cgcagttgca gttcgtgccg ctgtcatagt cggtatgcgt gcaggtccac gcgttcggta
     9061 cgcggtcgtg atcgacggcg tacccgccaa agttgccgca cttgcctcct ggcaccggcg
     9121 tcgagacgaa cgtctgctgc gcgatcgtga cctcctcgag ttgcaggtcg gtaagcgatc
     9181 cgacgaggcg cttgttcgtg aacgggtcct cggtcagcgt gatcgagccg cccgactggt
     9241 agtactgctt gacgacctga ccttgcgcat cgcgctcgaa cgcgcgcacg cagatcgcgc
     9301 acgtcgaata gttggactga tttcctgcct tcagatcgaa ggtgcccatc agggacgatt
     9361 caatgttgtc gtagaactcg aactggtatt cgaggttggt gccgagtccc gtggccgtga
     9421 tcggaccttt ccacgagacg aatccctgcg gatcgaatcc gttgaaatcg agcatccctg
     9481 gtgtcaccga ctcgcaggcc cgggaatcga ttcccatgtc gtggatgatg aacgcgtcag
     9541 gcgtcttgtt gttgttgttg ctgtcgtccc cgcaccctgc gaggagggca acgagcgcaa
     9601 taccagaggc acgtacgatg gtcattcgcc gaggctggca ggtagtcccg tcccggcgca
     9661 atggcatcgg tcacggatca actgttaggc tcgatccatg tcgaagctgg cggcactggg
     9721 agtaaccgta aagcggcagt acgtgacggt cgatggggcc gaaatgaccg acctcgtcgt
     9781 gaccctgccg aaatcgaccg gggtcgaagc cacgttttcc gccgaggact tcgcagacaa
     9841 ggtgttcaag gtcttcaagc gggagatcca ggtcggcgat ccgatcttcg acgaagcggt
     9901 tcacatcaag accgatacga ccgaccagac cgagaagctg ctgcagtcca gcgacatgcg
     9961 cgcgatcatc gaacgcgtga tcgtgaacgg cggcgcgatc gagatcgatg gcaacaccgt
    10021 gacgatgaag gtcgctggtc gtcaagagaa ggacgacgag gtgttcgagc tgttcgtgac
    10081 gacgctgctc ggttagccac cgacggcacc ggtcagatct gcatttgcat catgacgcgg
    10141 aactgcaggt cgggctggtc gggcgcgggc gtgtcaccga ggaactggat gaaaccgccg
    10201 tcgctggcga tcttccagat gtggccgtgc cagtagtagt tgaatgcgac gcgcgtttcg
    10261 atctccttgc ggtcgtccgc aatctctttg accgtgatca gcgcgaagcg ccccgccacc
    10321 tcgatcgtct ttggcgtgag gaagtagctg ggctgcacgt agaagccaac gttcgcatcg
    10381 gcggtcttca gcttctgcag ctcgacgccg gcttcgagcc cgaagcccca cgccttgatc
    10441 atcgtatcga tctcgaatcc gtggctcagg ttgtcagccc acgagtcttg cgcgcccttg
    10501 ttcaggttgc cgaggccgac cttgtatgac gcggcaaccg cataacggag cggaccgcct
    10561 tcgagatcgc tctcgctgta ccccttgatg ttcggcgtgt tgtagccgac gcgcgcgacg
    10621 agcgccggct cgaagtcgag cggaaaggtc gtcggtcggg tgttcgtgca cgcgatcttg
    10681 agcgtcaccg gatcctgcac gcacgctgtc gtccacgtcg gccgatcctg gccgccattg
    10741 agcgtgttga acatgccgac cgcccactcg atcggcgcag gtgacttctc gtaatcgttg
    10801 tgcagagcga tgccgaggtc acgaccacca cccgcttgct cgttctcgat cgaccgctcg
    10861 ttgaacgtgc tcgagaagtc gctcacgatc tccatgcggt tgtacgggcg tttccactga
    10921 ccgaagcgca ggtagatcgg agaatcgggc agccgcttct cgaggtacat gtccctgagg
    10981 aacgcgaagc tgccggaatc gcccataccg agctcggcct tgaagcggtt gtcgttgcca
    11041 aacagatgac cttcggcctg caggcgcgcg cgtgggatat agaaccgatt gaaccactgg
    11101 ttgtggcgcg tcggcgtttc gtcgtcgagc gggcggtttg cctcgaaccg cacctggttg
    11161 cggaactgga gcttgagctc gaactgatcg tccgccgtcg agaacttcat gccgttgtcg
    11221 tacttgatcg tgacggcacg cttttcttct gtcttcttct cgggctccgg cttgagcggc
    11281 gtctcctcga cgggcacggg cggctcggcc gctggcgccg tcggtgttgc cggcggctgc
    11341 tcctggtcgg ccggtggcgt cgccggtggt gggtcggtgg gtgctggctg cgcgacggcg
    11401 ccacgcgtcg cgaacagaac gaggccggca gtgaggatgt gcctgagcgt tgcggatcgc
    11461 gttgatcgca tgccgtgacg gtaccgtgag gcgcgcacga cgtgcgtcgc cgcccgctcc
    11521 cgcggccggc aaacgagaga tctccgacgt cgtcactgtc ccgtcacaca gccgccgctc
    11581 acgtgcaaca ccggggagct agggtgccac catgaagatg tggctcgcgt ctgtcgtgtc
    11641 gattggcctg atcgcctcgt gcggcaagaa gagggaagac ggcggcggca gcaccgggac
    11701 ggcgggcacc gcgagcggcg gcggcgcagc gtcggcgcga aaaataacga tcaatggcag
    11761 cgggtcgacg ttccaaaagg cctaccagga aatcgcgatc gagacgtacg cgaacgcgca
    11821 cggcgaagtc acagtgaatt atggcggcgg cggctcgggc aaaggccgcc aggacttcgc
    11881 cgacatggtc accgacttcg gttgtaccga cgcgccgttc aaagacgcgg agaagagcaa
    11941 gatcaagggc ggcgagctcg tctacgtacc ggtgctgctc ggcgcgatct cggtgagcta
    12001 caacgttccc gagatcgaga agctccagct ctcggcagag acactcgcca agatcttcca
    12061 gcgcgagatc aagaagtgga atgaccccgc gatcgcgcag gacaacccgg gcgcgaagct
    12121 gcctgcgacc gacatcgttg tcgcgcatcg ctcggatggc tcgggcacga ccgcgaactt
    12181 cacgctgttt ctgaccaaag cggcgggcgc agcgtggaag ctcaagagcg gctcgacggt
    12241 cgagtggtcg gccgacaccc aggccggcaa cggcaacagc ggcgtcgcgc agatcgtgaa
    12301 gagcacgcat ggcgcgattg gctacgtcga tctgccggat gcgaaggcgt ccgggctcaa
    12361 gtacgccgcc atcaagaacc aggccggcaa gtacgtcgag ccgacacctg agtcgaccca
    12421 ggctgccggc gaaggcatcg aggtcaaaga cgacctgacg ttcgaagctg tcaacgccaa
    12481 gggcgacgcg gcatatccga tcaccgcgca gagctggtgc atggcctaca cgaagcccgc
    12541 tgaccaggcg aagggcggcg cggtcaaggc gtactacaag tacctcgtca ccgacggtca
    12601 ggggctcctg aaggaaatcg acttcgcgcc gctgccgaag acgctgcagg acaaggcgat
    12661 cgcgcaggtc gagaagatcc agatctgatg gccgacaaga tcgatcttcg gggcacgccg
    12721 agccgcgccg accaggcctt ccgaggcctc gcgatgggtg cggcttgtct cgttctgctc
    12781 gtgctcgcgt tgatcgccat ccagatgtcg ggacgcatgg gcccggtctt cgacaggatg
    12841 ggcttttcgt acttcacgtc gaagcggtgg tcgccggcgg aggggctgtt cggtgccctg
    12901 cccttcttgt ggggcacgct gtacaccgcg tgcatcgcga tcgtgctgtc cgtcccggtg
    12961 agcctcttcg tcgcgctgtt cgtgacccag gtagcctcac cgcgctggcg caagccgatc
    13021 gtgtacgtac tcgacctgct cgcggtcgtg ccctcggtgg tctatggcct gtggggtgtg
    13081 ctcgtgctcg cgcagccgct gcagaagatc ttcctgaaga tccacaacgc ggtcgacggg
    13141 attcccggcc tgcagtcgat cttcggtccg acgccgcaag ggcgctcgtt catgaccgcg
    13201 ggcgtgatcc tcgcgatcat gatcacgccg atcatcacgt cgcttgcacg cgaagtgatc
    13261 gagacgacgc cgcagaacga gaaggaagcg gcgtacgcgc tcggtgcgac ccgctgggaa
    13321 atgattcgcg cgtcggtgtg gccgcacagc aagagcggcc tcgtcggtgc agtgatgctc
    13381 ggcctggggc gcgcgatggg cgagacgatc gcggctgcgc tcgtgattgg ctcgtcgatc
    13441 ggccagatca cgatcaacgc attcgctccc ggcaactcga tgcccgcggt catcgcgaac
    13501 gaatggggcg aagcaaatga cctccgcaag tcggcgatga tcgcgctcgc gttgctgcta
    13561 ttcgcgatca cgatcatcgt caacctcgtg tccagcgcga tcgtccagcg ctcgttgcga
    13621 cgcagtcgag gtgcgttgtg aggcatcccg gcgatccggt cgtcgcgatc agtctcgagc
    13681 cgcggctgtc gtggcgcacg atccagaact ggctgatgac cggcctcatg atcgccgcgt
    13741 tcgtcgcgat cacgattccg ctgatcgcga tcctgtactc gctggtatcg cgtggcgccg
    13801 cgatcgcgtt gcgcggtttc cccgatttct tcaccgcgac gattccgatc gtgtcgcgac
    13861 gcgccggacc ggggatgggc cccgcgatcg tcggtaccct gatctgcacc ggtggcgcaa
    13921 cgatcatcgc cgtaccgctc ggcattctcg gcgcggtcta cctgcacgag tacggcaagc
    13981 agtccaagtt cgcgcgcctc gtacgcttca tggcgatggt gatgaccggt gtgccctcga
    14041 tcgtcatggg cctgttcgtc tacgtgctgt ggacgatgcg cttcgggtat tcagcgttcg
    14101 gtggcgggct tgcgcttgcg tgcttgatgt tgccgatcat catcggcgcc accgagcaga
    14161 tgctgcgcct cgtccctgac catctgcgcg aggcggcgtt cgcactcggt gcgagcaaga
    14221 gtcgcgtgat cctgacggtc gcgttgcctg ccgcattgcc cggcatcgtg tcgggttgcc
    14281 tactggcgat cgcgcgcgca gccggtgaga ccgcgccact tctgttcacg attggcgcgg
    14341 cgacgaagtt caaccctcac atcttcgaca acgccaacac ggcgctgtcg gtacagatct
    14401 tcggcaacgc gacgtcttcg tttgccgctg cgcaagaacg cgcatggggt gccgcgctga
    14461 cgctcgtcgt cttgacgttc ttgctgacgc tcgtcgctcg catcgtagcc gcgcggttcg
    14521 cggcaaagag gtaaccgtgc cagaggagtc gaccatgcaa acaatcatca tcgagccggc
    14581 gcacccgagc cgtccgagct atcgtgtgca tgtcgacgac cccatgcccg ctgtagagga
    14641 cgcacccgtc gttttcgcgc tcgacaagct cgaagtgttc tacggagcct ttcgcgccgt
    14701 ccgcgacgtc tcgctcgaca tccacaagaa cgagatcacg gcgttcatcg gtccgtcggg
    14761 ctgcggcaag accacggtcc tgcgctgcct gaaccggatg aacgatctgg tgcccggcgc
    14821 tcgcatcggt ggtcacatca cgtatcacgg catcgatctc tacggctcgg atgtctcggc
    14881 cgccgaagtg cgccgccgca tcggcatggt gttccagaag ccaaacccgt tccccaagag
    14941 catctacgag aacatcgcgt tcggtccgcg tatcaatggc atccgcgacc gcgggaagct
    15001 cgacgaggtc gtcgaaaagt cgctgcgtgc tgccgcgctg tgggacgagg tcaaagaccg
    15061 tctgcatagc tcggcgctcg cgatgtcagg cggccagcag cagcgcctct gcatcgcccg
    15121 cacgattgca gtcgagcccg aggtcgtgct catggacgag ccgtgcagcg cgctcgatcc
    15181 gattgcgacc gcgcgcatcg aggagctgat gcgccagctc aagcgcacgt acacgatcgt
    15241 gatcgtcaca cacaacatgc agcaggcggc acgtgtcagc gaccgcaccg cgttcttcac
    15301 ggcggaagtc gacccgaagg ccgagcgtcg caccggcgtc ctcgtcgagt acgacgtcac
    15361 ctcgcgcatc ttctcgaatc cgcgcgagga gcggaccgag aactacatca ctggccgctt
    15421 cggttagctg tccgcgtcac ggcccgctgg cgagctcctc gtcgagcttt ggcttgttga
    15481 ggtcgccgac ccacttcgcg acgacgagcg tcgcgacgcc attgccgatc acgttcgtga
    15541 gcgcacgcgc ctccgacatg aagcgatcga tgccgacgat caccgcgatg ccagcgacgg
    15601 ggacgtcacc gacggccttc agcgtcgccg ccagtacgac gagcccactg ccggtcacgc
    15661 cagccgcgcc cttcgacgtc aggagcaaca cggcgagcag cgccagctga tcggtgacgt
    15721 cgaggtgcgt attcgtggcc tgcgcgacga acatcgctcc catcgtgagg tagatcgagg
    15781 tgccgtcgag gttgaacgag tagccagcgg gaacgacgag accgacagtt gacgggccgg
    15841 cgccgagtcg ctcgagcttg tcgatcatgc gcggcagcac cgattccgac gacgacgtgc
    15901 caagcacgat gaacagctcc tcgcggatgt agcgcaggaa cgcgaggatc gagaagccgt
    15961 gcacccgcgc gacggtgccg aggacaccga agatgaagat gaggcacgtg ccgtagaagc
    16021 acgccatcag ctcgccgagc gcgacgagcg agtgcaggcc gttggcgccg acggtgaacg
    16081 ccatcgcgcc gaacgcaccg atcggcgcga ggtgcatgat gaggccgacg atcttgaaga
    16141 tcacctgacc gagctcctcg acgagatcga acaacgcctt accgcgctca ccgatcgcgt
    16201 gcagcgcaaa gccgaacaga attgcgacga gcagcacctg gagcagctcg ccgtgcacga
    16261 acgcgccgac gaacgtgtct ggaatgacat cgaggaagaa gtcgacggtc gacttcatct
    16321 tgcccggtcc cgcgtacttc gcgatcgatg caggatcgat cttcgacgga tcgatgttca
    16381 tgccgacgcc gggctgtacg aagttgacga taacgagacc gatcgcgagt gcgagcgtgc
    16441 tcgcgacctc gaagtagagc agcgcgagac cgccggcctt gccgacggac ttcatgctct
    16501 tcatgctcgc gatgccgccg acgatcgtgc agaagatcag cggcgcgatc gtcagcttga
    16561 tcagcttgat gaagccttcg ccgagcggcg cgagcttcgc cgcggtcgat ggccacagca
    16621 cgccgaccag cacaccgagt ccgatggcga cgagaacgcg aaagtacagc gtcgtgaaga
    16681 acggcttctt atgcacgcta gggcgtgacg gtaacatgca gctcttgcca cgcgaggccg
    16741 ccgcggctgt cgcgcagcac gagccacagc catgcgtcgc cggcggccgg tgtgtgccag
    16801 gtcgtcgata cgctcgtcgc agtgccgtcc tcgtccaccg cactggcatc gacggcgagc
    16861 tcgccgccca tcgcgaacca cgagacacgc atcgcctcgc ggcggtcgac gagcctctgg
    16921 gtcaacgcgt cgtaatacag gtacgtctcg acggcctcgg gcggccatgc cgcggtcagc
    16981 gtgacatcgc tgtccgcatg aacatcggcg agcgcgaacg gctcgagcgt cgggttcgcg
    17041 ttggccatgt agtggaggtc gtaatcgcgc g
//
LOCUS       DASUFX010000142         8508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_439760,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000142 DASUFX010000000
VERSION     DASUFX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439760"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VFV99_05670"
     CDS             <1..1505
                     /locus_tag="VFV99_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5058827.1"
                     /translation="ARGTYAGVIEKIPYLRELGVTVVELMPVFQKDPQASDYWGYMPV
                     SFFAPDRSYAPAEETAIDSFRAMVAALHEANIEVVLDVVYNHTAEGGTDGPIYSFKGL
                     DNSTYYQMRDGAYANYSGCGNSLNANNRYVRKMILDSMRYWSREMHVDGFRFDLASVF
                     TRNADGTINFEDPPIFGDISSHPSFDRARMIAEPWDGAGVYELGRAFPGTAWLQWNSR
                     FRDDVRRFVRGDAGLTGALVQRLYGSDDLFPDDVIDAYHAYQSVNYVTSHDGFTLCDL
                     VSYEQKRNEANGENNRDGPDANWSWNCGWEGDDGVPAHVIALRERQAKNLIALLMLAN
                     GTPMLRAGDEFLVTQHGNNNPYNQDNATSWLDWRRRDEHAGFWRFVQRMIAFRKAHPS
                     LSRSRFWREDVRWYGPRGVADLARPTIAMLLHGASQDDDDLYVMINGGTESVSFVVQD
                     ARDGWCVVVDTARPAPEDIELERPRALERPSYTVEPRSIVVLLRSTKPGA"
     gene            complement(1502..2002)
                     /locus_tag="VFV99_05675"
     CDS             complement(1502..2002)
                     /locus_tag="VFV99_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058828.1"
                     /translation="MLGQLFLLACIAAALSVVGIFFVLFGPVPVKRRVTIVPRLAVPV
                     VAPAMPSLAAQLAPSREQHFTPTFAVESAPVLDYEPPTLQTPHAPVGVKPKGQKVQPL
                     PKKRSAKGTGSPAPFAPVVRARQYEDRRDVREDRRPYYREEEVQTNPVRRVSFDSGEM
                     TTVDDY"
     gene            2164..3351
                     /locus_tag="VFV99_05680"
     CDS             2164..3351
                     /locus_tag="VFV99_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058829.1"
                     /translation="MALLFSAVGCLDDKGPESDLPDLDGKADTQRKPTDHGGIAFGTP
                     VTSALTATAMYHAWTFELSADAHVDLTTSYAVLGQRRTDTVLYLYKQTATGTWGSYVA
                     RNDNYGSTTYSQLKKDLGAGRYRVLVKGYTETTYGKFKLTAMCSGAGCFNTSCAFGTT
                     YGEIFENPTMAIINQNVITPATLSTLNDVDRQHVVDAVKQSAHTDVTTPEEAIGRVDQ
                     MEINVTWFHEPAAQRAFVAFEYGAGDNSYGAIFGRTDDAMAARIHDGDLELCTVKNET
                     CLLPEDWSALKTDPAFTRTSTRVITAASQLTGIESQQALATFQRTYDGQVSSVADGLA
                     MADGNELNVVKFTHAATGTQLIVFEHGAGDTSVGVVFYSGELRRAGTIDDLFIGNCTF
                     FAH"
     gene            3483..4013
                     /locus_tag="VFV99_05685"
     CDS             3483..4013
                     /locus_tag="VFV99_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058830.1"
                     /translation="MRMLSAVLVLAATATTAHAEEAPTPAETEAPKEAGRPSEKGTLG
                     LGLIFGEPTGITGKLYLKDDQAVQGAFGASFYADAWQLHGEYVWHPWILQDRDTFVLP
                     VYLGPGMRFIRYNGGRGGDAEYAADLRAVAGLLFDFKNVPLDVFIEVGGAFGYNFSDS
                     GVDLGLNIGAGIRYYP"
     gene            complement(4010..7612)
                     /locus_tag="VFV99_05690"
     CDS             complement(4010..7612)
                     /locus_tag="VFV99_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5058831.1"
                     /translation="MKSELAELERYEIRRALGSGGLGVVYEAVDRESGATVAIKTLHD
                     VTPEALYRLKREFRLLQGLEHPNVCQHYELFEHAGDWFIAMECVRGDHILHAIRDEQG
                     DFDNDKLRDAMLQLAEALEALHEADLVHRDLKTSNVLVEPTGRVVLIDFGFVEASRDE
                     GAVQRSQTIVGTPVYMAPEQAVEAEVGPAADWYSFGVILFEALTKQLPHDGETALAVM
                     LAKQKTDPPRASSIAPDVPPDLDALCFELLRADPAKRPSGQGVLRRLGKREGTRDSRP
                     AMTSQASLAGSFIGRRAELETLRGALGDSERGKTVSVLVEGPSGVGKSALVRHFADEA
                     IARGALVLGGRCYERESVPYKAFDSVIDALARHLRRLSDVDVAALLPRNPELLVRMFP
                     VLGGVPAMSSVHSRVPVGEPHEQRNQAFAALREVLHRLGQRRALVITIDDWQWADADS
                     VVLARDLVRHRDSPPMLLVVTARPSEDSTTRLEAVATPDTRRIELEALVEAQAIELVD
                     QLRKAFAPTLDLDLAGIARETHGHPLYISELVRYAATRGSAGEPDKAIHLDEAILARI
                     AELPLEARAIVDVLSVAGEPLPIDVVRDAVDFDAAAVQRNAAMLRVAHLVRSANLDGA
                     LEPYHDRVSEAVLASMDDKQRLTWHKRIVYAIEASPLARVRPELLLRHLDTIGEGARA
                     AEIAIAAAQRAVIAGAFDQAASLYALALRTGNFDDLDARTLRIEMGQALANAGRGHEA
                     AQAFLDAAFESDPATRMDCQRQAAEQLLMVGDLERGLDILRKLLADFGVKMPTTQKRA
                     LFSVLWGRTKLRVRGLHWKERRETEIAPETLLRLDVLRAASHSLAMVDTIRGADFNAR
                     WLSLALGVGERSRVTVAIATESILQASYDKRSVARAMKLVETVRQLAAGSNNPLLTGW
                     VAMCEGSCDYWACRLPTAEQGIREAERVFRENAGTASELKSARMFLTFILRHRGSWSQ
                     LRDLREELVEAAERRGDRYVVTSMNRYCVSVWLAEDDPDTARRVVADAKWMLPNISFH
                     TQHWYELDARAEIAMYARTVERDMPEIAPLFDGLDSSVLLRVTTVHAMAFWLRGRLAL
                     CLGDRKAAQRAVDALTKNDNDRARVCGAMIEAGIAGRSRDVVAVAKLREATKLAEQLD
                     LRLHGAAARYQLGKLLAGSEGAEHIAAAEKIMVAEGVAKPAHFANWFVPGLDKI"
     gene            7642..8499
                     /locus_tag="VFV99_05695"
     CDS             7642..8499
                     /locus_tag="VFV99_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5058832.1"
                     /translation="MHEELGTRTADGVTLRVDRVPAQGVRRGAVVCLHAMMTDGRYFG
                     ARRDGSFAASLATAGLDVYVADFRGHGHSVPPYAGEGDWSFDDLVELDLPAILNLVSE
                     ASNLPAHEISILGHSLGGLVACAALGTGKIASPRVLVLAATSVWLGETASRRALMAAY
                     RGVTALFGRAPIKALRAGTADESVSYVGQLTSWAMQGRWTSLRGVDYLVALETITTPT
                     YAFAGAGDWMCKPFDAGAIAHRIRTCEPLRVVGRKHGDRLDPDHFQLFTRPELTKLRR
                     EVADKLTPA"
BASE COUNT         1450 a         2849 c         2779 g         1430 t
ORIGIN      
        1 cggcgcgcgg cacgtacgcc ggagtgatcg agaagatccc gtacctgcgc gagctcggcg
       61 tcaccgtggt cgagctgatg cccgtgttcc aaaaggatcc ccaggccagc gactactggg
      121 gctacatgcc ggtgagcttc tttgctcccg accgcagcta cgcgcccgcc gaggagaccg
      181 cgatcgattc gttccgcgcg atggtcgccg cgctacacga ggcgaacatc gaggtggtgc
      241 tcgatgtcgt ctacaaccac accgccgagg gtggtaccga cggcccgatc tacagcttca
      301 agggcctcga caacagcacg tactaccaga tgcgcgatgg cgcatacgcc aactattcgg
      361 gatgcggcaa cagcctcaac gcaaacaacc gctacgtccg gaagatgatc ctcgacagca
      421 tgcgctactg gtcgcgcgag atgcatgtcg acggtttccg cttcgacctc gcatcggtgt
      481 tcacgcgcaa tgcggacggc acgatcaact tcgaggaccc gccgatcttc ggcgacatct
      541 cgagccatcc ctcattcgat cgtgcacgca tgatcgccga gccgtgggat ggcgccggcg
      601 tgtacgagct cggtcgcgcg ttccccggca cggcgtggct ccagtggaat agccgcttcc
      661 gcgacgacgt ccgccggttc gtgcgcggcg acgctggact gacaggtgca ctcgtccagc
      721 gcctctacgg tagcgatgac ctgttccccg acgacgtgat cgacgcgtac cacgcgtacc
      781 agagcgtcaa ttacgtgaca tcgcatgacg gcttcacgct ttgcgacctc gtctcgtacg
      841 agcaaaaacg gaacgaggcc aacggcgaga acaaccgcga tggccccgac gccaactgga
      901 gctggaactg cggctgggaa ggcgacgacg gcgtaccggc ccacgtcatc gcactacgtg
      961 agcgccaggc aaagaatctg atcgcgctgc tcatgctcgc caacggcacg ccgatgctcc
     1021 gcgcgggcga cgaattcttg gtgacgcagc acggcaacaa caatccgtac aatcaggaca
     1081 acgccacgag ctggctcgat tggcgtcgcc gcgacgagca cgcggggttc tggcgattcg
     1141 tgcagcgaat gatcgcgttc cggaaggcgc atccgtccct gtcgcgcagc cgattctggc
     1201 gtgaggacgt tcgttggtac ggtccacgcg gagttgccga cctcgcgcgt ccgacgatcg
     1261 cgatgctgct ccatggtgca tcgcaagacg acgacgacct ctatgtgatg atcaacggtg
     1321 gaaccgagtc ggtgtcattc gtcgtgcagg acgcgcgcga tggctggtgc gtggtcgtgg
     1381 acaccgcgcg gccagcgccc gaggacatcg agctcgagcg cccgcgcgcg ctcgaacggc
     1441 cctcgtacac ggtcgagcca cgctcgatcg tggtcctgct tcgctccacg aagccaggcg
     1501 cttagtagtc gtcgaccgtc gtcatctcgc cggagtcgaa cgacacgcgg cgcaccggat
     1561 tcgtctggac ttcttcctca cggtagtaag gccggcgatc ctcgcgcacg tcgcgacggt
     1621 cctcgtactg gcgcgcgcgc acgaccggtg cgaacggcgc tggtgatccg gttcccttcg
     1681 cggagcgctt cttcggcagc ggctggacct tctggccctt cggctttacg ccgaccggcg
     1741 cgtggggcgt ctgcaacgtc ggcggttcgt agtcgaggac cggcgcggac tcgaccgcga
     1801 aggtcggcgt gaagtgctgc tcgcgcgacg gcgccagctg ggcggcaaga ctcggcatcg
     1861 cgggagcgac gaccggcact gcaagccgcg gcacgatagt cacccgccgc ttgaccggta
     1921 ctggcccaaa cagaacgaag aagatgccca cgacgctcag cgccgcggcg atgcaggcga
     1981 ggagaaagag ttggcccagc acggctcgct gaagtgcaat tctcagtccg cgattttgcg
     2041 gctcaactgc gcgagaaagc tcgcggaaca gcgcacccca ctgggggtcg tcaccccgga
     2101 ttttctcctc gacctgggag tcgtccggtt gatagaactc gcccccttat gcgcctagct
     2161 ttgttggctc tgttgttttc cgccgtcggc tgcctcgacg acaaaggtcc cgagtcggac
     2221 ctccccgacc tggacggtaa ggccgatacc cagcgcaagc cgaccgacca cggcggcatc
     2281 gcgttcggca cgcccgtgac gtcggcgctg accgcgaccg cgatgtacca cgcgtggacg
     2341 ttcgagctct cggcagacgc gcatgtcgat ctgaccacga gctacgccgt gctcggccag
     2401 cgccgcaccg acacggttct ctatctttac aagcagacgg cgacgggcac gtggggcagc
     2461 tacgtcgcgc gcaacgacaa ctacggcagc acgacgtact cgcagctcaa gaaggatctc
     2521 ggcgccggcc gctatcgcgt gctcgtcaag ggctacaccg agacgacgta cggcaagttc
     2581 aagctgaccg cgatgtgcag cggcgccggt tgcttcaaca cgagctgcgc gttcggcacg
     2641 acgtacggcg agatcttcga gaacccgaca atggcgatca tcaaccagaa cgtgatcaca
     2701 ccggcgacgt tgtcgacgtt gaacgacgtc gatcgccagc acgtcgtcga cgccgtgaag
     2761 cagtcggcgc acacggacgt aacgacgccc gaagaagcga tcggccgcgt cgaccagatg
     2821 gagatcaacg tgacgtggtt ccacgagccc gccgcgcagc gcgcgttcgt cgcgttcgaa
     2881 tacggtgccg gcgataactc gtacggcgcg atcttcggcc gcaccgatga cgcgatggcc
     2941 gcgcggatcc acgatggtga cctcgagctg tgcacggtca agaacgagac ctgcctgttg
     3001 cccgaggatt ggtcggcgct gaagaccgac ccggcattca cgcgcacgag cacgcgcgtg
     3061 atcacggcgg cgagccagct caccggcatc gaatcgcagc aggcgctcgc gacgttccag
     3121 cgcacgtacg acggtcaggt atcgtcggtc gccgacggtc tcgcgatggc agatggcaac
     3181 gagctcaatg tcgtgaagtt cacgcacgcg gcgaccggca cacagctcat cgtgttcgag
     3241 cacggtgcgg gagacacatc ggtcggcgtt gtgttctaca gcggcgagct gcgccgcgcg
     3301 ggcacgatcg acgatctgtt catcggcaac tgcacgttct tcgcgcactg aagcacgatc
     3361 gaaatttgtc ccacgcggac gcattccccg cagcgagctc gccatcgtca ctgcgcgacg
     3421 acggctcgtc gatccgtgcg gggaatttgc gtcgcaccgt cgcggcgggc taggttcgcg
     3481 ccatgcggat gctgtctgcc gtgctcgtgc tggccgccac tgcgacgacc gcgcatgccg
     3541 aggaagcgcc gacgcccgcc gagaccgagg cgccgaagga agccgggcgc ccgagcgaaa
     3601 aaggcacgct cggccttggc ctgatcttcg gcgagccgac cggcatcacc ggcaagctgt
     3661 acctaaaaga cgatcaggcc gtgcaaggtg cattcggcgc gtcgttctac gcggatgcgt
     3721 ggcagctgca cggcgaatac gtgtggcacc cgtggatcct gcaagaccgc gacacgttcg
     3781 tgctgccggt ctatctcggc ccgggcatgc ggttcattcg ctacaacggc gggcgcggcg
     3841 gcgacgcaga gtacgcggct gacctgcgtg ccgtcgcggg cctgttgttt gacttcaaga
     3901 acgtgccgct cgacgtcttc atcgaggtcg gcggcgcgtt cggctacaac ttcagcgatt
     3961 ccggcgtcga cctcggtctc aacatcggcg ctggcattcg ctactacccc tagatcttgt
     4021 cgagccctgg cacgaaccag tttgcgaaat gcgcaggttt ggcgacgccc tctgccacca
     4081 tgatcttctc tgcggccgcg atgtgctcgg cgccctcgct gcctgcgagc agcttgccga
     4141 gctggtagcg cgccgccgcg ccgtgcagcc gtagatcgag ctgctcggca agtttcgttg
     4201 cctcgcgtag ctttgcgact gccacaacat cgcgagagcg gccggcgatg ccggcctcga
     4261 tcatcgcgcc gcagacacgc gcgcgatcgt tgtcgttctt cgtgagcgcg tcgacggcac
     4321 gttgcgccgc cttgcgatcg ccgagacaca acgcgagccg gccgcgcagc cagaacgcca
     4381 tcgcgtgcac ggtggtcacg cgcaaaagga ccgaactgtc gagtccgtcg aacagcggcg
     4441 cgatctccgg catatcgcgc tcgacggtcc tcgcgtacat cgcgatctcg gcgcgtgcat
     4501 cgagctcgta ccagtgctgc gtgtgaaacg aaatgttcgg cagcatccac ttcgcatcag
     4561 caacgacgcg gcgagcggtg tccggatcgt cctcggcgag ccacaccgag acgcaatagc
     4621 ggttcatcga cgtcacgacg tagcgatcgc cgcgacgctc tgcggcttcg accagctcct
     4681 cgcgaaggtc gcggagctgg ctccacgacc cgcgatgccg caggatgaat gtcaggaaca
     4741 tgcgggcgct cttcagctcc gacgcggtgc cggcattttc tcggaacacg cgctcggcct
     4801 cgcggatacc ctgttccgcc gtcggcaacc ggcatgccca gtagtcgcag ctaccctcgc
     4861 acatcgcgac ccaaccagtg agcagcggat tgttcgaacc ggcggcgagc tgtcggacgg
     4921 tctcgaccag cttcatcgcg cgcgcgacgc tgcgcttgtc gtacgacgcc tggaggatac
     4981 tttcggtcgc gatcgcgacg gtgacacgcg accgctcgcc gacgcccaat gcgagcgaca
     5041 gccagcgtgc gttgaaatcc gcgccgcgaa tcgtgtcgac catcgcgagg ctgtgactcg
     5101 cggctctgag cacgtcgagg cggagcagcg tctcgggtgc gatctcggtc tcacggcgct
     5161 ccttccaatg aagaccgcgc acgcgcaact tcgtgcgtcc ccagagcacc gagaacagcg
     5221 cgcgcttctg cgtcgtcggc atcttcacgc cgaaatcggc gagcagcttg cgcaagatgt
     5281 cgaggccgcg ctcgagatcg ccgaccatga gcagctgctc ggccgcttgg cgctggcagt
     5341 ccatgcgcgt tgccggatca gactcgaacg cggcgtcgag aaacgcttgc gcagcttcgt
     5401 gaccgcgacc ggcgttcgcg agcgcctgtc ccatctcgat gcgcagtgtt cgcgcgtcga
     5461 gatcgtcgaa gttgcccgtg cgaagcgcga gagcgtacaa gctcgccgct tgatcgaatg
     5521 cgcctgcgat gactgcacgc tgcgcggccg cgatcgcgat ctctgccgcc cgcgcaccct
     5581 cgccgatcgt gtcgaggtgg cgtagcagca gctccggccg cacgcgtgca agcggcgatg
     5641 cttcgatcgc gtagacgatg cgcttgtgcc acgtgagccg ctgcttgtca tccatgctcg
     5701 cgagcaccgc ttcgctgacg cggtcgtgat acggctcgag cgcgccgtcg aggtttgcgc
     5761 tgcgcacgag gtgagcgacg cggagcatgg cggcgttgcg ctgcacggca gcggcatcga
     5821 aatcgacggc atcgcggacg acgtcgatcg gcagcggctc gccggcgacc gacagcacgt
     5881 cgacgatcgc gcgcgcctcg agtgggagct cggcgatacg cgcgagaatc gcctcgtcga
     5941 ggtgaatggc cttgtcgggc tcaccggccg agccgcgcgt cgcggcatag cggacgagct
     6001 ccgaaatgta gagcggatgg ccgtgggtct cgcgcgcgat tcccgcgaga tcgaggtcga
     6061 gcgtcggcgc aaatgccttg cgcagctgat cgacgagctc gattgcctgg gcctcgacga
     6121 gcgcttcgag ctcgatgcgc cgcgtatccg gcgtcgcgac tgcctcgagc cgtgtcgtgc
     6181 tgtcttcgga cgggcgcgcc gtgacgacga gcagcatcgg cgggctgtcg cgatgacgca
     6241 cgagatcgcg cgcgagcacg acgctgtcgg cgtcggccca ctgccaatcg tcgatcgtga
     6301 tcacgagcgc tcgtcgctga ccgagccggt gcagcacctc gcgcagggcg gcgaacgctt
     6361 ggttacgctg ctcgtgcggc tcgccgaccg gaacgcgtga atgcacgctg ctcatcgccg
     6421 gtacgccgcc gaggacgggg aacatgcgga ccaggagctc gggattgcgc ggtaacagcg
     6481 cggcgacgtc gacatccgag agccggcgca agtggcgcgc gagcgcgtcg atcacgctgt
     6541 cgaatgcctt gtagggcacc gactcgcgct cgtagcagcg gccgccgagc acgagcgcgc
     6601 cgcgcgcgat cgcctcgtcg gcaaagtgac gaacgagcgc gctcttgccg acacccgatg
     6661 gcccctcgac gagaaccgac acggtcttgc cgcgctcgct gtcgccgagt gcgccgcgta
     6721 gcgtctcgag ctccgctcgg cgaccgatga acgaaccggc gagcgacgcc tgtgatgtca
     6781 tcgccggtcg gctgtcgcgc gtgccttcgc gcttgccgag ccggcgcagc acgccttgcc
     6841 ccgacggtcg cttcgccggg tccgccctca acagctcgaa gcacaacgca tcgaggtcgg
     6901 gtggcacgtc gggcgcgatg ctcgacgcgc gcggcggatc ggtcttctgc ttcgcgagca
     6961 tgactgcgag cgcggtctcg ccgtcgtgcg gcagctgctt cgtcagtgcc tcgaacagga
     7021 tgacgccgaa gctgtaccaa tcggccgcag gaccgacctc ggcttcgacc gcctgctcgg
     7081 gcgccatgta caccggcgtg ccgacgatgg tctggctgcg ctgaaccgcg ccctcgtcgc
     7141 gactcgcttc gacgaacccg aagtcgatca gcacgacgcg cccggtcggc tcgacgagca
     7201 cgttcgacgt cttcagatcg cgatgcacga ggtccgcttc gtgaagcgcc tcgagtgctt
     7261 cggcgagctg gagcatcgca tcgcgcaact tgtcgttgtc aaagtcgccc tgctcgtcgc
     7321 gaatcgcgtg caggatgtgg tcgccgcgca cgcactccat cgcgatgaac caatcgcccg
     7381 cgtgctcgaa cagctcgtag tgctggcaca cgttcggatg ctcgaggcct tgcagcagcc
     7441 ggaactcgcg cttcaggcgg tacagcgcct ccggggtcac gtcgtgcagc gtcttgatcg
     7501 cgacggtcgc gcctgactcg cgatcgaccg cttcgtagac gacgcccagt ccgcccgagc
     7561 ccagtgcgcg gcggatctca tagcgctcca gctcagccag ctccgacttc aacgtgtaat
     7621 cacgactata gtacgagcgg gatgcacgaa gagctgggaa cgcggacggc ggatggggtg
     7681 acgcttcggg tagatcgcgt ccccgcgcaa ggcgtgcggc gcggggctgt ggtctgccta
     7741 cacgcgatga tgacggatgg gcgttatttt ggtgcccgcc gcgacggttc gtttgctgcc
     7801 tcgctggcga ctgcaggtct cgacgtctac gtcgctgatt ttcgtggcca cggacattcg
     7861 gtgccaccgt acgccggtga aggcgactgg agcttcgacg acctggtcga gctcgacctt
     7921 ccggcgatcc tgaatctggt gtccgaagcc tcgaacctgc ccgcccacga gatttcgatc
     7981 ctcggacatt ctctgggtgg cctcgtcgcg tgtgcggcgc taggcacggg aaaaatcgca
     8041 tcgccgcgcg tgctcgtgct cgccgcgacc tcggtgtggc tcggtgagac cgccagccgc
     8101 cgcgcgctca tggcggccta tcgcggtgtc actgcgctat tcggtcgcgc accgatcaaa
     8161 gcgctgcgcg ccggtaccgc ggacgaatcg gtgagctacg ttggccagct cacgagctgg
     8221 gcgatgcaag gccggtggac gagccttcgc ggcgtcgatt acctggtcgc tctcgagacg
     8281 atcacgacgc cgacctatgc gttcgccggc gccggcgact ggatgtgcaa accgttcgat
     8341 gccggcgcga tcgcgcatcg aatccgcacc tgcgaaccat tgcgcgtcgt cggacgcaag
     8401 cacggtgacc gactcgatcc cgatcacttc cagctgttca cgcggcccga gctcacgaag
     8461 ctgcgccgcg aggtcgccga caagctcact cccgcgtgag tcgtgata
//
LOCUS       DASUFX010000143         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1411777, whole genome shotgun sequence.
ACCESSION   DASUFX010000143 DASUFX010000000
VERSION     DASUFX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411777"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV99_05700"
     CDS             <1..342
                     /locus_tag="VFV99_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058833.1"
                     /translation="ERVAAYEHEHGYFAEHSTPERYLESNWALLDGTTLRNPPGATRG
                     IDPSARVDASARIVEPVRICANAVIAANVTVGPYAVVGEGARIERSIERTVVWSGATA
                     DASNAGEPIVS"
     gene            423..1022
                     /locus_tag="VFV99_05705"
     CDS             423..1022
                     /locus_tag="VFV99_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5058834.1"
                     /translation="MSKGEETRQAILSRAFELATVVGVSGLSIGRLAEEMGLSKSGLF
                     AHFGSKEALEVAVVEEASRQFVQEVMVPALRHPRGEPRLRALFDGWMQWGQRPGGCFF
                     VGASTELDDRPGAPRDTLVRALKDWLDELAKAVRISISEGHFRADVNPEQVAFEIYGL
                     MLGMHTFHRFLREPTTLDRARQAFERLLTDSRVASPRSR"
     gene            1061..2011
                     /locus_tag="VFV99_05710"
     CDS             1061..2011
                     /locus_tag="VFV99_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5058835.1"
                     /translation="MSTPKNSTVVRSKNAVRLARAALQTAYVVSEDLGTSLAERLFTT
                     PRRHRRPDRERNVLATAREGSIDITLRAPRWHNMRVPVATWRWGVGPTVLLVHGWEGR
                     GSQLGAFVAPLVAAGMSVVTFDAPGHGNSPEHRLYLTDHADAIADVAAAVGPLHGIIA
                     HSFGAAAALLAFTRRGTHAPRNVMVAPNVLIDDAVRRFAKMVGLDEADRIALEQRLAT
                     QTGVGIGDLRLPNIAAQRDDALLIYHDREDREVSFEHASQLAATWPNAQLVETAGLGH
                     RRILREGAVLAAIVEFVRDGISLPQSDLVREVDRQLEGQP"
     gene            2008..2496
                     /locus_tag="VFV99_05715"
     CDS             2008..2496
                     /locus_tag="VFV99_05715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5058836.1"
                     /translation="MKVARSYEYEARTPDIAVMLSMPGLDYLRGVLRGDFPAAPIAAT
                     LNFFPTEFDPGRAVFEGIPERFAYNPLGDIHGGWAATILDSALGCAVHSTLPAGKYYT
                     TVDLSVSLVRAITEKTKRVRCEANIIHSGSSVATAEAKLVDDRGNLCAHGTTTCLVMV
                     PR"
BASE COUNT          423 a          885 c          772 g          431 t
ORIGIN      
        1 gagcgcgtcg cggcatacga gcacgagcac ggctacttcg cggaacactc gacacccgag
       61 cgctatctcg aatcgaactg ggcgttgctc gacggcacca cgctgcgcaa tccgcctgga
      121 gcaacacgcg gcatcgatcc gagcgcgcgt gtcgacgcga gcgcgcgcat cgtcgagccg
      181 gtgcgcatct gcgccaatgc agtgatcgcg gcgaacgtga ccgtcggccc gtacgcggtc
      241 gtcggcgagg gcgcgcggat cgagcgttcg atcgaacgca ccgtcgtgtg gtccggagcg
      301 accgcggatg cgagcaacgc cggcgagccg atcgtcagct aactagtgcc ccggactcgg
      361 gtttgtcttc atcggcgatc gcagacattg acaaatcgaa cgaccgttct attctaactt
      421 tcgtgagcaa gggcgaagaa acccggcaag cgatcctgtc ccgagcgttc gagctcgcga
      481 cggtcgtcgg cgtttcgggc ctctcgatcg gccgcctcgc ggaagagatg ggcctctcga
      541 agagcggtct gttcgctcat ttcggttcca aagaagcgct cgaggtcgcg gtcgtcgaag
      601 aggcgtcgcg ccagttcgtg caagaggtca tggtgccggc gctgcggcat ccgcgcggcg
      661 agccacgcct gcgcgcactg ttcgatggtt ggatgcagtg gggacagcgc cccggcggct
      721 gcttcttcgt cggcgcgagc accgagctcg atgatcggcc cggcgcaccg cgcgacacgc
      781 ttgtccgcgc tctcaaggac tggctcgacg agctggcgaa ggccgtgcgc atctcgatca
      841 gcgagggcca cttccgcgcc gacgtcaatc ccgagcaggt cgcgttcgag atctacggcc
      901 tcatgctcgg catgcatacg tttcatcggt ttctgcgcga gcccactacg ctcgaccgcg
      961 cacgtcaggc gttcgaacgc ctgctcacgg attcccgcgt agcgtccccg cgttctcggt
     1021 agcagttcca cacgagatca aggaggttcc tgtgcagcac atgtcgacgc ccaaaaatag
     1081 cacggtcgtt cgttctaaaa acgccgtccg cttggcccga gctgcgctcc aaactgcgta
     1141 cgttgtctcc gaggatctgg gtaccagtct cgccgaacga ctgttcacca caccgcgccg
     1201 acaccggcgt ccggaccgcg aacgcaatgt tctcgcgacc gcgcgcgaag gcagcatcga
     1261 catcacgctt cgcgcaccgc gctggcacaa catgcgcgtc ccggtcgcga cgtggcggtg
     1321 gggtgtcgga ccgaccgtac tgctcgtgca cggctgggaa ggtcgcggct cgcaactcgg
     1381 tgcgttcgtc gcgccgctcg tcgccgccgg aatgtcggtc gtcacgttcg atgcacctgg
     1441 tcacggcaac tcgcccgagc atcggctcta tctcaccgat cacgcggacg cgatcgccga
     1501 cgtcgccgcc gcggtcggcc cgctgcacgg catcatcgcg cactcgttcg gtgctgccgc
     1561 cgcgctgctc gcattcactc gtcgtggcac gcacgcgccg cgcaacgtga tggtcgcgcc
     1621 gaacgtgctg atcgacgacg ccgtgcgtcg cttcgcgaag atggtcggcc tcgacgaagc
     1681 cgatcgcatc gcgctcgagc agcgactcgc cacgcaaacc ggcgtcggca tcggcgacct
     1741 tcgcctgccg aacatcgccg cccagcgcga cgacgcactg ctcatctatc acgatcgcga
     1801 ggaccgcgaa gtttcgttcg agcacgcgtc gcaactcgcc gcgacgtggc cgaacgcgca
     1861 gctcgtcgag acggccggcc tcggtcaccg ccgcattctg cgcgaaggcg ctgtgctcgc
     1921 tgccatcgtc gagttcgtac gtgacggcat ctcgctgccg caatccgatc tcgttcgcga
     1981 agtcgatcgt caactcgaag gtcagccatg aaagtcgcac gcagctacga atacgaagca
     2041 cgcacgcccg atatcgcggt catgctctcg atgccgggcc tcgattactt gcgtggcgtc
     2101 ctccgcggcg actttcccgc tgcaccgatc gctgccacgc tcaacttctt cccgacagag
     2161 ttcgaccccg gccgcgcggt cttcgaaggc atccccgagc gcttcgcata caacccgctc
     2221 ggcgatattc acggcggctg ggcggccacg atcctcgact ccgcgctcgg ctgcgccgtt
     2281 cactcgaccc tccccgcggg caagtactac acgaccgtcg atctctcggt ctcgctcgtc
     2341 cgcgcgatca ccgagaagac aaagcgcgtc cgctgcgaag ccaacatcat tcattcgggc
     2401 agcagcgtcg cgaccgccga ggccaagcta gtcgacgacc gcggcaacct ctgcgctcac
     2461 ggcacgacca catgcctcgt catggtgccg cgctaaatcc ggcgtcgcat c
//
LOCUS       DASUFX010000144         4807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1411803, whole genome shotgun sequence.
ACCESSION   DASUFX010000144 DASUFX010000000
VERSION     DASUFX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411803"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFV99_05720"
     CDS             complement(<1..565)
                     /locus_tag="VFV99_05720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5058837.1"
                     /translation="MQREKVQLEGEKQTLLVTLYGKALDSRKLHPILGDTYAADVVDH
                     LDVDFEKLRIPKGADVSLPVRAKHLDGWTREFLIAHPTATVLHLGCGLDSRVWRVDPP
                     ATVRWYDVDYPDVIELRKRLYPERHDYTMIGTSVTDVHWLDAIPRDNPVLVVAEGLVQ
                     YLKPADGIALFRSITETFLSGELIFDAY"
     gene            complement(641..1246)
                     /locus_tag="VFV99_05725"
     CDS             complement(641..1246)
                     /locus_tag="VFV99_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX5058838.1"
                     /translation="MQDCEPIREVLAKFLPSQGTVLEIGSGSGERVIHFARHLKALTW
                     QPTAADPDTLATLTQRVSEAALENLLPPLDLDVQRQPWPGSQVAAVLAIDVVNTMPWE
                     NVLAMFFGASRSMLSAGLLFIYSAVRFNGRYTAKVNEDRDQALRARDEKFALRDIREL
                     TVAGTRTGFGLERTLAMPDDYHSLVFKRRQILPPSGAFNIG"
     gene            1300..3288
                     /locus_tag="VFV99_05730"
     CDS             1300..3288
                     /locus_tag="VFV99_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058839.1"
                     /translation="MALDRRLVIALAVSFGLAGQAYAEAKKTFSVEVVAAEAWRADAL
                     GTVLRSDLVDDRQALAETKADIVVHVDVEADGLHYELRALWAHAPAPVRGVIAAGASR
                     TDVAGVLRDRMHRLARHAKGETDAGSALELPAVGTVLLALAGLLALLALPLVFVARAR
                     GGVRAALPALREAAVSIAIAGGAAVVLAIVGDVPNAEGIVVGAGGLAWGTWLVATFPI
                     VCPPLVGLWRVEHEALPRVLRLWLVVVVQRVGAVTVLYGPFALVTWFVGDALGLAPAT
                     TVALAIPLALIAVRMWLRVGVAVAAVALDRALVDEDANISAWNAATRAYLVGYHTRNG
                     LPLDEHLLSRVQFLPGRGDAVAVYGGGLTDSRIVVPRKLLELALAPAGRPHDYAAPRV
                     SILSWTHWNVGLVMASEPGAVGATREQRQPGQLAVEGEYERVLIGEPPTLGYVVEPSA
                     LDERKDHRPHDDPMWLEWDPGDEYDGTDAGDRDFLFGLLVHALGRIRRHEERLHTLRV
                     LAMRVPWLARIGERLKRATRVLLRPSASLADEHAALGGARHHLVQYLAWQLWRRDDLL
                     TARAYAPELVLTTRRVCSELAAKASVPDPELAQRVQRLGALVPGVPATRATRWGRFAV
                     AAVALAGVGLATFAIVDAVSYHPTYVEHVKAETHGKTK"
     gene            3272..4057
                     /locus_tag="VFV99_05735"
     CDS             3272..4057
                     /locus_tag="VFV99_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5058840.1"
                     /translation="MAKQSENVAGLALGESSAGAGALGLIGAAAVFIVAIVFFVAESY
                     FLAMLLVSFGMLVYVAGAELGKLLRSSFTVFFSGRHLIKNATYVQDTVTALRKHLRMK
                     RDEQGWIKVGPIESGQKLKLPDNPFVREIQAVLKRKKGKEYAQYVAHQYYVDCRELYD
                     HLHAHLEFVASVMPLFGLIGTVVGLIGMFDRLGSNTSVESLAPELAISLQCTLWGAIL
                     ASAYMSAASRFDQRIKALEYDYDILCNALDVLVENNADIEVEA"
     gene            4054..4677
                     /locus_tag="VFV99_05740"
     CDS             4054..4677
                     /locus_tag="VFV99_05740"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5058841.1"
                     /translation="MTRRRPHNTGRKGGHGALWSIIYIDLMTQIMAFFVIVWTVEKGT
                     KATQTAAIGTGIGMGNQTVRMVDLPGDVLFASGKTDVADEGKKVIGELFGDPSSGVLS
                     FDQGGLAERKLVIHGHTDDVGDKDKNFQLGYERAYAVYREIRAHSTEIPDHVVLCTHA
                     DNTPANDVPVAAKPDDATKAAAASARSKNRRITIEDLVVDKRRQGAE"
BASE COUNT          765 a         1483 c         1721 g          838 t
ORIGIN      
        1 tgtacgcgtc gaagatgagc tcgccgctca gaaacgtttc cgtgatggat cggaacagcg
       61 cgatcccgtc agccggcttc agatactgca cgagcccttc ggcgacgacg agcacggggt
      121 tgtcgcgcgg aatcgcatcg agccagtgca cgtcggtgac cgacgtgccg atcatcgtgt
      181 agtcgtgacg ctcgggatac aggcgtttgc gaagctcgat cacgtcggga tagtcgacgt
      241 cgtaccagcg cacggtggcc ggcggatcga cgcgccacac gcgactgtcc aaaccacacc
      301 cgagatgcag cactgtcgcg gtcgggtggg cgatgagaaa ctcacgcgtc cagccgtcga
      361 ggtgcttcgc gcgcaccggc aacgagacat ccgcgccctt cggaatccgc aacttctcga
      421 aatcgacgtc gagatgatcg accacgtccg ccgcgtacgt gtcgccaagg attggatgca
      481 acttgcggct atcgagcgct ttgccgtaga gcgtgacgag gagcgtctgc ttctcgccct
      541 cgagctgaac cttctcgcgc tgcatgctcg agtctaccgc cgacgaggca gcggacgagt
      601 cgagcgttac gcgctcctaa cttgccaacg tgttcgacgt ctaaccgatg ttgaacgcgc
      661 cgctcggtgg gaggatctgg cggcgcttga acacgagcga gtgatagtcg tcgggcatcg
      721 caagcgtgcg ctctaggccg aagccggtgc gtgtgccggc gacggtcagc tcgcggatgt
      781 cgcgtagcgc gaacttctcg tcgcgcgcgc gaagcgcttg gtcgcggtct tcgttgacct
      841 ttgcggtgta gcggccgttg aagcggacgg cagagtagat gaagagcaag cccgccgaga
      901 gcatcgagcg cgatgcgccg aagaacatcg cgagcacgtt ctcccacggc atcgtgttca
      961 cgacgtcgat cgcgagcacg gcggcgactt gcgagcccgg ccatggctgg cgctggacgt
     1021 cgagatcgag cggcggcagg aggttctcga gtgccgcttc gctgacgcgc tgagtgaggg
     1081 ttgcgagcgt gtccggatcg gccgcggtcg gctgccaggt gagggccttg aggtggcgcg
     1141 cgaagtggat gacgcgctcg ccactcccgc tgccgatttc gaggacggtt ccttgggacg
     1201 gcaggaactt cgcgagcact tcgcggatgg gttcgcagtc ctgcatttcc ggcattgtag
     1261 gcgcgatgtt gggggggcgt gttagctcga tcgggtctga tggcactcga tcgacgcctc
     1321 gtgatcgcgc tcgccgtgag ctttgggctc gccggccagg cgtatgcgga ggcgaagaag
     1381 acgttttcgg tcgaggtggt ggcggccgag gcgtggcgcg cagatgcgct cggcaccgtg
     1441 ctgcgcagcg accttgtcga cgatcggcag gcactcgccg aaacgaaggc cgacatcgtg
     1501 gtgcacgtcg acgtcgaggc ggatgggctg cattacgagc tgcgcgcatt gtgggcacac
     1561 gccccggcgc ccgtgcgtgg ggtgatcgcc gcgggcgcga gccgaacgga tgtcgccggc
     1621 gtgctgcgcg atcgcatgca ccgactcgcg cgccacgcga agggtgagac cgatgcgggg
     1681 agtgcgctcg agctgccggc ggtggggacc gtgctgctcg cgctcgccgg cctactcgcg
     1741 ttgctggcgc tgccgctggt gtttgtcgcg cgcgcgaggg gtggcgtgcg cgcggcgttg
     1801 ccggcgttgc gggaggcggc ggtgtcgatc gcgatcgcgg gcggtgcggc ggtcgtgctc
     1861 gcgatcgtcg gcgacgtacc gaatgcggag ggaatcgtgg tcggcgccgg cgggctcgcg
     1921 tgggggacgt ggctcgtcgc gacctttccg atcgtgtgcc ctccgctcgt cgggctgtgg
     1981 cgcgtcgagc acgaggcatt gccgcgcgtg ttgcggctgt ggctcgtggt cgtcgtgcag
     2041 cgcgttggcg cggtgaccgt gctgtatggc ccgttcgcgc tcgtgacgtg gtttgtcggc
     2101 gatgcgctcg ggcttgcgcc ggcgacaacc gtcgcgttgg cgattccact cgccttgatc
     2161 gcagtgcgca tgtggctgcg cgttggcgtc gcggtggcgg cggttgcgct ggaccgcgcg
     2221 ctcgtcgatg aggatgcgaa tatcagcgcg tggaacgcgg cgacacgtgc gtatctggtc
     2281 ggctatcaca ctcggaatgg attgccgctc gacgagcatt tgctgtcgcg cgtgcagttc
     2341 ttgcccggta gaggcgatgc agtggcggtg tacggcggcg ggctcaccga cagccgcatc
     2401 gtggttccgc gcaagctact cgagcttgca ctcgcgccgg ccgggcggcc gcacgattac
     2461 gccgcaccgc gcgtgtcgat cctgagctgg acgcactgga acgtcggcct cgtgatggcg
     2521 agcgagcccg gtgcggtggg cgcgacacgc gaacagcgcc agcccggtca gctcgcggtc
     2581 gagggcgagt acgagcgcgt gctgatcggc gagccgccga cgctcggcta tgtcgtcgag
     2641 ccgagcgccc tcgacgagcg caaggaccat cggccgcacg acgatccgat gtggctcgag
     2701 tgggatcccg gcgacgagta cgacggcacc gacgcaggcg atcgcgactt cttgtttggc
     2761 ttgctcgttc acgcgctcgg ccggatccgg cgccacgaag aacgattgca tacactgcgc
     2821 gtgctcgcaa tgcgcgtgcc gtggctcgcg aggatcggcg agcgactgaa gcgcgccacc
     2881 cgtgtgctgc tgcggccctc ggcgtcgctc gcggacgagc acgctgcact cggcggtgcg
     2941 cgtcatcacc tggtgcagta cctcgcgtgg cagctgtggc ggcgcgacga tctgttgacc
     3001 gcgcgcgcat atgcgccgga gctcgtgctg acgacgcggc gcgtgtgtag cgagctcgct
     3061 gcgaaggcga gcgtgcctga cccggagctc gcgcaacgcg tccagcggct gggcgcgctg
     3121 gtgcccggcg tacccgcgac gcgcgcgacg cgctggggac gattcgcagt tgccgcggtc
     3181 gcacttgcag gcgtcggcct cgcgacgttc gcgatcgtcg acgctgtttc ttatcacccg
     3241 acctacgtgg agcacgtgaa ggcagagact catggcaaaa caaagtgaaa acgtagccgg
     3301 gttggcactc ggcgaatcga gcgcgggcgc gggtgcactc gggctgatcg gtgccgcggc
     3361 ggtgttcatc gtcgcgatcg tgttcttcgt cgccgagagc tacttcctcg cgatgctgct
     3421 cgtgtcgttc ggcatgctcg tgtatgtcgc gggtgccgag ctcggcaagt tgctgcggtc
     3481 gtcgttcacg gtgttcttct cgggacggca cttgatcaag aacgcgacgt acgtgcagga
     3541 cacggtgact gcgctgcgca agcacctgcg catgaagcgc gacgagcaag gctggatcaa
     3601 ggtcgggccg atcgagtcgg gccagaaact gaagctgccg gacaacccgt tcgtgcgcga
     3661 gatccaggcg gtgctcaaac gcaagaaggg caaggagtac gcgcagtacg tcgcgcacca
     3721 gtactacgtc gattgccgcg agctgtacga ccacctgcat gcgcacctcg agttcgtcgc
     3781 gtcggtgatg ccgctgttcg gtctgatcgg caccgtggtc gggttgatcg gcatgttcga
     3841 tcgtttggga tcgaatacgt cggtcgagag cctggcaccc gagctcgcga tctcgctgca
     3901 gtgcacgctg tggggcgcga tcttggcgtc ggcgtacatg agcgcggcgt cgcgcttcga
     3961 tcagcgcatc aaggcgctcg agtacgacta cgacatcttg tgcaacgcgc tcgacgtgct
     4021 cgtcgagaac aacgccgaca tcgaggtcga ggcatgacgc gccggcgccc acacaacacc
     4081 ggacggaaag gcggtcacgg cgcgctgtgg tcgatcatct acatcgacct gatgacgcag
     4141 atcatggcgt tcttcgtgat cgtatggacc gtcgagaaag gtacaaaggc gacgcagacc
     4201 gcagcgatcg gcaccggcat cggcatgggc aatcaaaccg tgcgcatggt cgacctgcca
     4261 ggcgacgtgc tgttcgcgtc gggcaagacc gacgtcgccg acgaaggcaa gaaggtgatc
     4321 ggcgagctgt tcggcgatcc gtccagtggc gtgttgtcgt tcgatcaagg cgggcttgcg
     4381 gagcgcaagc tcgtcattca cggccacacc gacgacgtcg gcgacaagga caagaacttc
     4441 cagctcggct acgagcgcgc gtacgcggtc tatcgcgaga tccgagcgca ctcgacagag
     4501 attcccgacc acgtcgtgct gtgcacgcac gccgacaaca cgcccgcgaa cgacgtgccg
     4561 gtcgctgcga agcccgacga cgccacgaag gccgccgccg ctagtgcgcg cagcaagaac
     4621 cgccgcatca cgatcgaaga tctcgtcgtc gacaagcgac gacagggagc cgagtagcgt
     4681 tgcgtttgct cggcgccaag agcatggcag cgatcgtgct cgcgaccgcg ctcgtcatcg
     4741 cggcggcgct gaccggcaag ctgtcgcctg cgcacctcgc gcacctgagc cccggcgcgt
     4801 gcctcgc
//
LOCUS       DASUFX010000145         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1134224, whole genome shotgun sequence.
ACCESSION   DASUFX010000145 DASUFX010000000
VERSION     DASUFX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134224"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..949)
                     /locus_tag="VFV99_05745"
     CDS             complement(263..949)
                     /locus_tag="VFV99_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058842.1"
                     /translation="MGYGLDLFPAPAGSTAAELRIIFEGRDVLTSADKDMGSYTRPPC
                     AEQVRISHLLRDRFPSLEVTLSCENDIELTENRLGVQIYIFQREAGISLHPGGYSTAL
                     YIESLQLTWASIEILQRELGLATYDTQIERFLDLKTDFEAVLACYGGSKAVKLYRSQR
                     EKVEQSHVYQLTKALETRSATPYNATASYAVGDVVQHPTLGLGVVRVLGPKRMTILFE
                     TGTKILVCGT"
     gene            complement(1249..1330)
                     /locus_tag="VFV99_05750"
     tRNA            complement(1249..1330)
                     /locus_tag="VFV99_05750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1294..1296),aa:Leu,seq:gag)
     gene            1420..2019
                     /locus_tag="VFV99_05755"
     CDS             1420..2019
                     /locus_tag="VFV99_05755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5058843.1"
                     /translation="MSYEPSPPPPLSRASLVIGLYAAMGIVAFLIAAGRGNPDLYRLG
                     GSPEAWQLGAGPAIGVALGLAVVGLTRVATRNFQWARELHTSFRDLLGPLTAREIVIL
                     ALASSIGEELLFRGALLPWFGVWIQAAVFAALHIGPGRRFLPWTMSALVLGIGFGLIA
                     KWTGNLGGPIAAHFVINYLNLKYIVGTDPVRAVAAIDAG"
     gene            complement(2020..2628)
                     /locus_tag="VFV99_05760"
     CDS             complement(2020..2628)
                     /locus_tag="VFV99_05760"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5058844.1"
                     /translation="MTERLPAEVLARRADLPKKPAAITLTGTLVTVRPLDLDADVDEL
                     HAVSSGVDCDARVWRYMSGGPFASAAELRAWLAHQDAADDGRPFTVWMRGQRVGVANL
                     IANQPQHLKIELGSIWYAPHAQRTGASREVTALLLAHVFGLGYRRAEWKCDALNEPSR
                     RAALSYGFTFEGIQDAHYIIKDHNRDTAWYRMLDHEWRARRS"
     gene            2649..>4098
                     /locus_tag="VFV99_05765"
     CDS             2649..>4098
                     /locus_tag="VFV99_05765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058845.1"
                     /translation="MKPGEVVEDRFALERVAGSGGMGTVYRAIDRTTGTPVAFKVLRS
                     ASQDAASRFAREVRILSSLRHPGIVRYIADGRTADGEMWLAMEWLDGESLNQRLARTG
                     VSASESVELVRRVAEALGAAHERGVIHRDLKPSNLFLMDNDLERVKVLDFGVARIADA
                     SATRTGVMIGTPGYMAPEQARGERNVGARSDVFALGCLLFECLTGKPPFVGDNVMAIL
                     AKVLLEEAPRVGVVRQDLPEALDDLVARTLSKAPEARPRDGAQLAAELAALGTITAAE
                     RPQNVNVAALTATERRLLCVVLVDVGTEAPVDLDATRSSVPSLEVDATMPSTPSHDTI
                     AEQHRPELKPLREAAEAHGARLQRLVDGSLVVTLAGTGGTADQAMQAARCALALKGQL
                     AGTPMALATGRGVMAGRWPVGEAIDRAAALLAAARKASPHLVRVDEVTAGLLDTRFDV
                     GGDEAGLYLLGERDVVQATRTLLGKPTPCVGRD"
BASE COUNT          667 a         1308 c         1381 g          742 t
ORIGIN      
        1 agcacgtcgg cttcggcgag cggctgcacc gtgcagagct gcccgctcga aatcttgccg
       61 accatgccgt taccgcgcgg tctgaattgc acggtctcgc ctcgcgccag acgcgcgatg
      121 tggcagtctg cccatcccat gagttccttc gcttcgaagg aagccgagca ccgtcatcca
      181 ggcgaactgc gccgctccgg tcatccgcgc ggcgatgacc gcgcccttct cccccgccag
      241 tagcaatcga gcgcatcgga tgctacgtgc cgcagacaag gatcttcgtt cccgtttcga
      301 acaggattgt cattcgcttc ggaccgagga cgcggacgac gccgagtccg agcgttggat
      361 gctgaacgac gtcgccgacg gcgtaggacg cggtcgcgtt gtatggtgtt gcgcttcggg
      421 tctcgagtgc ctttgtaagc tggtagacgt ggctctgctc caccttctcc cgctgcgagc
      481 ggtagagctt cacggccttc gagccaccgt agcacgccag caccgcttcg aaatcggtct
      541 tcaaatcgag gaagcgctcg atctgggtgt cgtaggtcgc gagaccgagc tcgcgctgga
      601 ggatctcgat gctggcccag gtgagttgga gcgactcgat gtacagcgcc gtgctgtagc
      661 cgccgggatg caggctgatt ccagcttccc tctgaaagat gtagatctgg acccccagtc
      721 ggttctcggt cagctcgatg tcgttctcac aggaaagcgt cacttcgagc gagggaaagc
      781 gatcgcgtag gagatgggag atgcggactt gctcggcgca tggcggcctc gtgtagctgc
      841 ccatgtcctt atcggcggac gtgagtacat cgcgaccctc gaagatgata cggagctccg
      901 ctgccgttga acccgctggt gccgggaaca gatccagtcc atagcccacg cggcgagcat
      961 ggcatagtga gcgttcactt cgcacagccc ttctggggtc ggcgacagcg caagtcacac
     1021 cagtagcgag aggcgtgtca acgggctacg tccgcgaggg cggcctgccc ctgcattttg
     1081 agaagagccg ttagttggct ccggaggagg gtttcgaagc cccacacggt taagtactag
     1141 cacccaaagc tagcgcttct cccaattcca ccacccgcgc tgttcgcgtt gtctcccgaa
     1201 aacgaaacaa gcggcattgc tgccgctcga tcggtttctc ggggttcgtg cgcgaggggg
     1261 gactcgaacc cccacacggt taagtactag cacctcaagc tagcgcgtct accaattccg
     1321 ccacccgcgc aattcagttg tcaccgaagg gctgtttgag taccgctcga cgctacctct
     1381 gtcaagcgcc gcccaatcct gcatagtatg gggccggcca tgtcgtacga gcccagcccg
     1441 ccaccaccac tgtcgagggc gagcctcgtc atcgggctct acgcggccat gggtatcgtg
     1501 gcgttcttga ttgccgctgg ccgcggcaac cctgacctct accgcctcgg cggaagtccc
     1561 gaagcgtggc agcttggcgc tggccccgcc atcggcgttg cgctcgggct tgccgttgtc
     1621 ggcctgaccc gcgtggcgac gcgtaacttt cagtgggcgc gcgagcttca tacgagcttt
     1681 cgggatttgc tcggcccgct gaccgcgcgc gagatcgtga tcttggcgct cgcgagctcg
     1741 atcggcgagg agctgttgtt tcgcggggcg ctgctcccgt ggttcggggt gtggattcag
     1801 gcggccgtgt tcgcggcgct gcacatcggg ccgggccggc gcttcttgcc gtggacgatg
     1861 tccgcgctcg tgctcggcat cggctttggt ctgatcgcca agtggaccgg caatctcggt
     1921 ggcccgatcg ccgcgcactt cgtgatcaat tatctgaacc tgaagtacat cgtcggcacc
     1981 gacccggtgc gcgcggtcgc cgcgatcgac gcgggctaat cacgagcgcc tggcgcgcca
     2041 ctcgtgatcg agcatgcggt accaggcggt atcgcgattg tgatccttga tgatgtagtg
     2101 cgcgtcctga atgccttcga acgtgaagcc gtacgagagc gcggcacggc gcgacggctc
     2161 gtttagcgcg tcgcacttcc attcggctcg tcggtaaccg agtccgaaga cgtgcgcgag
     2221 caagagtgcg gtgacttcac gggaggcacc cgttcgctgc gcgtgcggcg cgtaccagat
     2281 actgccgagc tcgatcttta ggtgctgcgg ttgattcgcg atcagattcg cgacaccgac
     2341 gcgttggccg cgcatccaga ccgtgaacgg ccggccgtcg tcggcggcgt cttggtgagc
     2401 gagccatgcg cgcagctcgg ccgcgctcgc gaacggtccg cccgacatgt agcgccacac
     2461 gcgggcatcg cagtcgacac cgctcgacac ggcatgcagc tcgtcgacgt cggcgtcgag
     2521 atcgagcggc cgtacggtaa cgagcgtacc agtgagcgtg attgcggcgg gcttcttcgg
     2581 caggtctgcg cgacgcgcga gcacttcggc agggagacgt tcggtcacgt gttagcttta
     2641 gcagagccat gaaacccggg gaggtcgtgg aggaccgctt cgcactggag cgtgttgcgg
     2701 gctctggtgg catgggcacg gtgtatcggg cgatcgatcg cacgactgga acgccggtcg
     2761 cgttcaaggt gctgcgcagc gcatcgcaag acgcggcgtc gcggttcgcg cgcgaggtgc
     2821 gaatcctgtc gagcctgcgt catcctggca tcgtgcggta catcgccgat ggccgcactg
     2881 ccgatggcga gatgtggctc gcgatggaat ggctcgacgg cgagagcctg aaccagcgcc
     2941 tcgcacgcac cggcgtcagc gcgagcgaga gcgtcgagct cgtgcgacgc gtcgccgagg
     3001 cgcttggcgc agcacacgaa cgcggtgtca ttcatcgtga cttgaagcca tcgaacttgt
     3061 ttctgatgga caacgatctc gagcgcgtga aggtgctcga cttcggtgta gcgcgcatcg
     3121 ccgacgcgtc agcgacgcgc accggcgtca tgatcggcac gcctggctac atggcgcccg
     3181 agcaagcgcg cggcgagcgc aacgtcggcg cgcgctcaga cgtgttcgcg ctaggctgcc
     3241 tgttgttcga gtgcttgacc ggcaagccgc cgttcgtcgg cgacaacgtg atggcgatct
     3301 tggcaaaggt gctgctcgaa gaagcgccgc gcgtcggcgt ggttcgccag gatctgcccg
     3361 aagcactcga cgatttggtc gcgcgcacgc tgtcgaaggc gccggaggca cggccgcgcg
     3421 acggtgcgca gctcgcggca gagctcgcgg cactcgggac gatcactgcg gccgaacgac
     3481 cgcagaacgt caacgttgcg gcgttgaccg cgaccgagcg acgcctgttg tgcgtcgtgc
     3541 tcgtcgacgt tggcaccgaa gcccccgtcg acctcgatgc gacgcggtcg agcgtgccat
     3601 cgctcgaggt cgacgcgacg atgccgtcga cgccgtcgca cgacacgatc gcggagcaac
     3661 atcgccccga gctgaagccg ttgcgcgaag ccgcggaggc gcatggcgca cgcctgcaac
     3721 gactcgtcga cggctcgctc gtcgtcacgc tcgccggcac cggcggtact gcggatcaag
     3781 cgatgcaggc agcccgctgc gcgctcgcgc tcaaagggca gctcgcaggc acgccgatgg
     3841 cactcgcgac cggtcgcggc gtgatggccg ggcggtggcc cgtgggcgaa gcgatcgatc
     3901 gcgcggcagc gctgctcgcg gcggcgcgca aggcgtcgcc acatctcgtc cgcgtcgacg
     3961 aggtgaccgc cggtttgctc gacacgcgat tcgatgtcgg cggcgacgaa gccggcttgt
     4021 atctgctcgg cgagcgcgat gtggtgcaag cgacgcgaac gctgctcggc aagccaacgc
     4081 cgtgtgtcgg ccgcgatc
//
LOCUS       DASUFX010000146         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_717688,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000146 DASUFX010000000
VERSION     DASUFX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717688"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1394)
                     /gene="rimO"
                     /locus_tag="VFV99_05770"
     CDS             complement(33..1394)
                     /gene="rimO"
                     /locus_tag="VFV99_05770"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5058846.1"
                     /translation="MASATQRIYFVSLGCPKNQVDTELMLGQVEAAGHSLVHAPDDAD
                     VIVVNTCAFIDAAKEESVDTILEMAEYKKKTKRKLVVTGCLAQRYSDELSKDIPEIDH
                     ILGSADFQSIAKVLAPAKTRKKLPMVQVSETPEYIYDHDAPRVRIGANHSAYVKVAEG
                     CDRPCSFCIIPKLRGPQRSRAIADIVAEVEQLGREGVQEANLIAQDLTRYGWDTGSDP
                     ATRATLAQLLRALGKSDSAVKWIRLHYTFPSAFDDELIDVIASEPKVVKYIDVPLQHI
                     SDPMLKRMRRGHSSRITRELVTKLRERIDNVVLRTTFIVGHPGETDAEFDELCEFVAE
                     SKFDRAGAFTYSIEPGTVSAMLPNRVDPQVMAARQEKLMEIQRQISRARHEAMVGKTI
                     DVLVEGVSSESEYLQEGRWYGQAPGIDGMTYLADGPATPGTIVKARVTQGSDYDLAAS
                     LEL"
     gene            complement(1430..2827)
                     /locus_tag="VFV99_05775"
     CDS             complement(1430..2827)
                     /locus_tag="VFV99_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058847.1"
                     /translation="MRALSFLLVLVPSLAVAGIERRTIERAGSGAFPDDADELVYRLD
                     FTWQGQPTETSEISVRKSAIALNDQFRGDLRGMREGEVRRFSGHEIDGLTCDHFGCGP
                     AKRFTARIELTKVIAHHDRLPDGMHIAAHPLRVVDATDSAELGGDDVASIREVRVVGT
                     NVEIDVTYQPETRPGETRTMSIDELRARLADARAVLALRRGELRSARELLDHALALAP
                     SLDEARAHRANVLLRTDGADAANEALTDLAARRPVWLLYRVSTDKLLAGLAGLPAVKA
                     LTTAGGNVEPSAFEERDLVVLAEPSGRYLGVYWTATYTDWVVRVIDRQSARVVESVSV
                     MCSRGRIHRESLETCRAKSPGARVLAELGFAPTNTATMLGRYDAQVERIDDIEIIRTI
                     GRELTVKRVVASPPATIPATNVALATTAAPATTSTSAATEPRRPHAWPLAGLAAVLLI
                     IVAARVSISGHKHPR"
     gene            complement(2870..3388)
                     /locus_tag="VFV99_05780"
     CDS             complement(2870..3388)
                     /locus_tag="VFV99_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5058848.1"
                     /translation="MKNAANPGAASRGKVTVERTFDSPIEDVWELWTTKEGIESWWGP
                     DGFAVVVHAIDLRPGGKLRYAMSAVQPEMVAYMKQQGMPTTNECTLTFTEVVPNQRLA
                     YLHLADFIPGVEPYEVVHVVELQQTPAGVRMTLTFDTMHADEWTERAKQGWENEVNKL
                     GRALATRAKRPA"
     gene            complement(3385..3717)
                     /locus_tag="VFV99_05785"
     CDS             complement(3385..3717)
                     /locus_tag="VFV99_05785"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5058849.1"
                     /translation="MHIDVFQTLADPTRRQIVEALRGGEHPVNDIVDRLHIHQSGVSR
                     HLRILQEAGFVRVRPDGAQRFYSLRPEPFRELETWIASYRDLWERRFDRFEQALERKK
                     QQRKGKQS"
     gene            complement(3782..4894)
                     /locus_tag="VFV99_05790"
     CDS             complement(3782..4894)
                     /locus_tag="VFV99_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058850.1"
                     /translation="MGWSVTYELRRTAKLTAEERTWLEQHIARHEKRGYDLHIAPKRS
                     VLASGMAKVPFDAEHDEWRDLLVAITELRRRFSNATCKVADTYDCIYWDEELDAYANG
                     MRDGTEELGDADNDADDDEDDGAEILDDEAEDEVVGRLQLAPEAIAAPFNVSLDARLE
                     GQNLTIDIGRTIVGVKLQITQIRFVARAADGAPCFSKLLASPSGERLYVSEDFADIVS
                     SFELFATVRRDLNELVASYDAKASIAAKSALAPIELTRRESAAPIGIELVSVDGWTAS
                     EFGDQEIKLFATAKAALPLAFDGTLVFRLLDDAGNELEHGDGLLRSPIELVRAGRSPL
                     PQSTITALRRIDVLAKGRLEGQLPLGRFRIVKPASS"
     gene            complement(4894..>5584)
                     /locus_tag="VFV99_05795"
     CDS             complement(4894..>5584)
                     /locus_tag="VFV99_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058851.1"
                     /translation="ASLALAGDVAVTTVERRLRLAGTATSALPIKLEYIYATLRDAGG
                     RVIGIPDGSCRSRTGETHELALEQFIGASTLAATRSIELSVPYKIEVSNELAIARLGP
                     PAGDGRVPLDITRRGAVPFVDIVSIAAYFNAGAPIAAYRGLQVFVELDVRRGARGTYC
                     TVSGRVRLDGDKVLGEERAYPGGPDSDTTRRIYEIPFRYTEGTATQLELAITVDAVGI
                     GRLGPIAVEGL"
BASE COUNT          844 a         1844 c         1890 g         1006 t
ORIGIN      
        1 ccgtgctgcc gctgccgcgc cgatcttgag ggctacagct cgaggctggc ggcgaggtcg
       61 tagtccgagc cctgcgtgac gcgagctttg acgatcgtgc ccggggtggc cgggccgtcg
      121 gcgaggtacg tcatgccgtc gataccaggc gcctggccgt accagcgtcc ctcctggagg
      181 tactcgctct cgctagacac gccctcgacg agcacgtcga tcgtcttgcc gaccatcgcc
      241 tcgtggcgcg cgcggctgat ctgccgctgg atctccatca gcttctcttg gcgcgcggcc
      301 atcacctgcg gatcgacgcg gttcggcagc atcgctgaca ccgtgcccgg ctcgatcgag
      361 tacgtgaacg cgccggcgcg atcgaacttc gactcggcga cgaactcgca aagctcgtcg
      421 aactcggcgt cggtctcgcc gggatggccg acgatgaacg tggtgcgcag cacgacgttg
      481 tcgatgcgct cgcgcagctt cgtgacgagc tcgcgcgtga tgcggctcga gtggccgcgg
      541 cgcatgcgct ttagcatcgg gtcgctgatg tgctggagcg gcacgtcgat gtacttgacg
      601 accttcggct cgctcgcgat gacgtcgatc agctcgtcgt cgaatgcgct cgggaacgtg
      661 tagtggaggc ggatccactt cacggcgctg tcgctcttgc cgagcgcgcg gagtagctgc
      721 gcgagcgtcg cgcgcgtcgc cgggtcgctg ccggtatccc agccgtagcg cgtgaggtcc
      781 tgcgcgatca ggttcgcttc ctgaacgcct tcgcggccga gctgctcgac ctcggcgacg
      841 atgtcggcga tcgcgcggct gcgctgcggc ccgcgcagct tcgggatgat gcagaacgag
      901 cacggacgat cgcagccctc agccaccttc acgtaggcgc tgtggttggc gccgatgcgc
      961 acgcgcggtg cgtcgtgatc gtagatgtac tcgggcgtct cgctgacctg gaccatcggc
     1021 agcttcttcc tcgtcttcgc gggtgcgagc accttcgcga tcgactggaa gtcggcggag
     1081 ccgaggatgt gatcgatctc gggaatgtcc ttcgacagct cgtcggagta gcgttgcgcg
     1141 aggcagccgg tgacgacgag cttgcgcttc gtcttcttct tgtactccgc catctcgagg
     1201 atggtgtcga cggactcttc cttcgcggcg tcgatgaacg cgcacgtgtt gaccacgatg
     1261 acgtcggcgt catcgggcgc gtgcacgagc gagtggcccg ccgcctcgac ctggccgagc
     1321 atcagttcgg tgtcgacctg gtttttcgga caaccaagcg acacgaagta gatgcgttga
     1381 gtggcagacg ccatcgggcc gggcagtctg tgccgccgct cccgatgtgt caacggggat
     1441 gtttgtgccc ggaaatcgag accctggccg cgacaatgat gagcagaaca gcggccagac
     1501 ccgccagtgg ccacgcgtgg ggtcggcgcg gctcggtcgc ggccgaggtg ctggtcgtcg
     1561 ccggggcggc ggtcgtggct agcgcgacgt tggtagccgg tatggttgcg ggcggcgacg
     1621 cgacgacgcg cttcaccgtg agctcgcggc cgatggttcg aatgatttcg atgtcgtcga
     1681 tgcgctcgac ctgcgcgtca taccgcccaa gcatcgtcgc cgtgttggtc ggcgcgaagc
     1741 ccagctcagc gagcacgcgt gcgccgggag acttcgcgcg acaggtctcg agcgactcgc
     1801 ggtgtattcg cccgcggctg cacatcacgg acaccgattc gacgacccgt gcgctctggc
     1861 gatcgatcac gcgcacgacc cagtctgtgt acgtcgcggt ccagtacacg ccgaggtatc
     1921 gcccgctcgg ctcggccagc acgacgaggt cacgctcttc gaatgcggag ggctctacgt
     1981 tgccgcccgc ggtcgtgagg gctttgacag ccggtaggcc agcgagcccg gcgagcagct
     2041 tgtccgtgct cactcgatac aggagccaca ccggtcggcg cgccgcgagg tcggtgagtg
     2101 cctcgttcgc ggcgtcggcg ccgtcggtgc gcagcaggac attggcgcga tgagctcgag
     2161 cctcgtcgag cgacggtgcc agcgcgagcg cgtgatcgag caactcgcgg gcgctgcgca
     2221 gctcgccgcg gcgaagtgcg agcaccgcgc gtgcatcggc gagccgcgcg cggagctcgt
     2281 cgatcgacat cgtgcgggtc tcaccggggc gggtctcggg ctggtacgtc acgtcgatct
     2341 cgacattcgt gccgacgacg cgcacctcgc gaatcgacgc aacgtcatcg ccgccgagct
     2401 cggccgagtc ggtcgcgtcg acaacgcgca gcgggtgcgc ggcgatgtgc atgccatcgg
     2461 gcaagcgatc gtggtgcgcg atcaccttcg tcagctcgat gcgcgcagtg aatcgcttcg
     2521 cggggccaca gccgaagtga tcgcacgtca gcccgtcgat ctcgtgacct gagaaccgcc
     2581 gcacttcgcc ttcgcgcatt ccgcgcaggt cgccgcggaa ctggtcgttg agcgcgatcg
     2641 cgctcttgcg cacgctgatc tccgacgtct cggtcggttg gccctgccac gtgaagtcga
     2701 ggcgatagac gagctcgtca gcgtcgtcgg ggaacgcgcc gcttccggca cgctcgatcg
     2761 tgcgtcgctc gatgccagcg acggcgagcg acggtacgag gacgagcagg aaactgagcg
     2821 cgcgcatgtc gacgaaaacg cgccgccgca gcgcgctatt tcctgccgat cacgccggcc
     2881 gcttggcgcg agtcgcgagg gcacggccga gcttgttgac ctcgttctcc cagccctgct
     2941 tcgcgcgctc ggtccactcg tcggcgtgca tcgtgtcgaa cgtcagcgtc atgcgaacgc
     3001 cagcgggcgt ctgttgcagc tcgacgacgt gcacgacctc gtacggctcg acgcccggaa
     3061 tgaagtcggc gaggtggagg tacgcgagcc gctggttcgg cacgacctcg gtgaacgtga
     3121 gcgtgcattc gttcgtcgtc ggcatcccct gctgcttcat gtacgcgacc atctccggct
     3181 gcaccgcgct catcgcatag cggagctttc cgcccggccg cagatcgatc gcgtggacga
     3241 cgacggcaaa gccatcgggt ccccaccacg actcgatgcc ctccttcgtc gtccacagct
     3301 cccagacatc ctcgatcggc gaatcgaacg tgcgttcgac ggtgaccttg ccgcgcgatg
     3361 cggcacctgg attggcggcg tttttcatga ctgttttcct ttgcgctgtt gcttcttgcg
     3421 ttccagagcc tgttcgaacc gatcgaagcg gcgttcccac agatcgcgat acgacgcgat
     3481 ccacgtctcg agctcgcgga acggctcggg acgcagcgag taaaaccgct gtgcgccatc
     3541 gggccgcacc cggacgaagc ccgcctcctg caggatgcgc aggtggcgcg acacgccgga
     3601 ctggtggatg tgcaagcggt cgacgatgtc attgaccgga tgttcgccgc cgcgcagcgc
     3661 ctcgacgatc tgtcggcgcg tcggatcggc cagggtctga aacacatcga tatgcatgtc
     3721 tgtgcatatg catatcagtg catgtcttgc ctgtcaaccc ggacccgtcc gcggtgacgt
     3781 gctacgagct ggcgggcttg acgatccgga agcgaccgag cggcagctgt ccttcgaggc
     3841 ggccttttgc gagtacgtcg atgcggcgca gcgcggtgat cgtgctctgt gggagcggcg
     3901 accgtccggc ccgcacgagc tcgatcggtg agcgcagcag gccgtcgccg tgctcgagct
     3961 cgttgccggc gtcgtcgaga agcctgaaca ccaacgttcc gtcgaacgcg agtggaaggg
     4021 ccgccttggc agtggcgaac aacttgatct cctggtcgcc gaattcgctc gccgtccagc
     4081 cgtcgaccga aacgagctcg ataccgatgg gggccgctga ctcgcgccgc gtgagctcga
     4141 tcggtgcgag cgcgcttttg gccgcgatgc tcgccttcgc gtcgtacgac gcgacgagct
     4201 cgttaaggtc gcgacgtacg gtcgcgaaca gctcgaacga gctcacgatg tcggcaaagt
     4261 cctccgacac gtacaaccgc tcgccactcg gactcgcgag cagcttcgaa aagcatggag
     4321 cgccatcggc cgcgcgcgcg acgaatcgga tctgtgtgat ctggagcttc acccccacga
     4381 tcgttcgacc gatgtcgatg gtgaggttct ggccctcgag cctcgcatcg agggacacgt
     4441 tgaacggagc agcgattgct tcaggcgcga gctgcagccg accaaccacc tcgtcctcgg
     4501 cttcgtcgtc caggatctcg gcgccgtcgt cctcgtcgtc gtccgcgtcg ttgtctgcgt
     4561 cgccgagctc ctcggtgcca tcgcgcatgc cgttcgcgta ggcgtcgagc tcctcgtccc
     4621 agtagatgca gtcataggtg tcggcgacct tgcacgtggc gttcgaaaag cgccggcgca
     4681 gctcggtgat cgcgaccagc aggtctcgcc actcgtcgtg ctcggcgtcg aacggaacct
     4741 tcgccatgcc actcgcgagc acgctgcgtt tcggcgcgat gtggaggtcg tacccgcgtt
     4801 tctcgtgacg cgcgatgtgc tgctcgagcc aggtgcgttc ctctgcggtg agctttgccg
     4861 tgcgacgcag ctcgtatgtg accgaccagc ccatcagagt ccctccaccg cgatcgggcc
     4921 gagcctgccg attcccacgg cgtccacggt gatcgcgagc tcgagctgtg tagccgtgcc
     4981 ttcggtatac cggaacggga tctcgtagat gcggcgcgta gtgtcactgt cgggaccgcc
     5041 gggatatgcg cgctcctcgc ccagcacctt gtcgccgtcg agacgcacgc gaccagacac
     5101 ggtgcagtac gtcccgcgcg cgccgcggcg gacgtcgagc tcgacgaata cctggaggcc
     5161 gcgatatgcg gcgatcggcg caccggcgtt gaagtaggcg gcgatcgaca cgatgtccac
     5221 gaacggcacg gccccgcgcc tcgtgatgtc gagtggcacg cggccatcgc ccgccggcgg
     5281 cccgagccgc gcgatcgcga gctcgttcga cacctcgatc ttgtatggca cggacagctc
     5341 gatgctgcgc gtcgccgcca gggtcgacgc gccgatgaac tgttcgagcg cgagctcgtg
     5401 agtctcgccg gtgcggctcc ggcacgatcc atcgggaatg ccaatgacgc gcccgccggc
     5461 gtcacgcagc gttgcgtaga tgtactcgag cttgatcggc agcgcgctgg tcgcggtgcc
     5521 cgcgaggcga agccggcgct cgacggttgt cacggcgacg tcgccggcaa gcgcgagcga
     5581 tgcc
//
LOCUS       DASUFX010000147         9899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_857292,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000147 DASUFX010000000
VERSION     DASUFX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857292"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFV99_05800"
     CDS             <1..459
                     /locus_tag="VFV99_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEX5058852.1"
                     /translation="VKIAPDLADADIDAIADLALELALDGIIATNTTIARTGLTTPDS
                     RVQALGAGGLSGAPLKARSLDVLRRLRARVGDKLVLIAVGGIENGDDAWARIRAGATL
                     VQAYTGFIYGGPLWPRRVQRELAAHVRAAGLTSIEQAIGADVPSASSAAS"
     gene            complement(452..967)
                     /locus_tag="VFV99_05805"
     CDS             complement(452..967)
                     /locus_tag="VFV99_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058853.1"
                     /translation="MFACGGDDGGGGNGNPDAKVFMDSKVFMDAPGGGGGLTGLGQKC
                     GSGQPACPTNAPDCIALGLGGGSASTSYCTPHCLEDGSGTTNAQGQLTTTTPAPDNAK
                     CSAVYTGGSVGMPACGVILATTPMDNPLKANTSYTMIDLGCVIVCGTGMMCPTGTTAT
                     TAGSTCFCFPN"
     gene            1142..1714
                     /gene="def"
                     /locus_tag="VFV99_05810"
     CDS             1142..1714
                     /gene="def"
                     /locus_tag="VFV99_05810"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5058854.1"
                     /translation="MAIRKIAQIGHPVLRQTAREVSREELASVATQQFIDDLVETMRD
                     ADGAGLAAIQVYEPVRICAIEVRNNPRYPYKPQIPLTILVNPVLTPVGDEQFDNYEGC
                     LSVPNLRGKVKRYVHLRVQALDRNGNPIDEVVHGLKAGTYQHEVDHLNGKIFVDRADP
                     STFATWTEFERHHRAKFIEEAQALVARVGS"
     gene            1781..2443
                     /locus_tag="VFV99_05815"
     CDS             1781..2443
                     /locus_tag="VFV99_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5058855.1"
                     /translation="MARRSKSGSVAPKRGRSKKKPAGPLINVRHMTRRDIGDAWEFLK
                     VSFRAVNQETVEYQRPISRRRFEEVFEEEGIEQLLFEVNGEIVAYAECSYEVTGSDNW
                     INPRYFEKRDMKPLFVDELAVHPDWSGKGVGSFVLEQLHHLARVHGLNHLVLEVAENN
                     ERALNWYKKRSFKKLDAAIFMACEVEIEPELLPPRALPVRGSLPPPSSGDDGDGGDQT
                     ED"
     gene            2629..2874
                     /locus_tag="VFV99_05820"
     CDS             2629..2874
                     /locus_tag="VFV99_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058856.1"
                     /translation="MRSLDKRLLHAIRELARRERISLNKAALRLLERGAGLAPASQEN
                     RIGSSLDHLAGTWSALEAKAFEQALKSCEQIDEAMWK"
     gene            2871..3263
                     /locus_tag="VFV99_05825"
     CDS             2871..3263
                     /locus_tag="VFV99_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5058857.1"
                     /translation="MRRLLDTNAYVAWKRGDRSVMDIVNGSSELAFSTIVIGELLYGF
                     RHGNRFSKNANELDELLADARVSVLPVTTTTADRFGRIAAVLRKAGTPIPTNDIWIAA
                     HAFESGAELVSFDAHFESVPGLVWTQLG"
     gene            3372..4466
                     /locus_tag="VFV99_05830"
     CDS             3372..4466
                     /locus_tag="VFV99_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058858.1"
                     /translation="MGGWPTACAMLALAGCSFEHGVAPDELGSDGGYQDAVGTGLDGA
                     IGSSCYGPAAGAWRVCLGSVPTGGVMLPPTLDTTTSMLCLQAQPAEWTTSGQPPACFV
                     VGDAIQTSKTVVTGSRPLVLLSSGTITIGSLDLASHADSSATGPGADPSVCKPFAANP
                     QTGDGNGGGAAGSFMTKGGDGGDGHDGDEKGGRAATADGTAPAVLRGGCRGQKGGGFS
                     GGAGGHGGGAAYLVAATSIMVTGTINASGAGGSAGNNNKPGGGGAGSGGMIVLYAPLI
                     GVTGTVFANGGGGGGGGDGDGSPGDDPLFAGVTAGGGSPAGGDGYALSVNATSGGRDK
                     DGGGGGGGGAGYIRSNLTIGATSPAADIVP"
     gene            complement(4477..5253)
                     /locus_tag="VFV99_05835"
     CDS             complement(4477..5253)
                     /locus_tag="VFV99_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058859.1"
                     /translation="MTELSRSLIAAAREGLAPDDAVAARVRAKVAAAVGATAATGAAA
                     AIPAAKVGSSSLLLKLGAALLAVGAVATVVVVARPNRGGEAPRVDVAPRAGDEVRSEV
                     RVVAPAEPPLQPDRSQPVVSQPEHHAHVQSTSDAVEDGAESREPDDGAASAHPSKARG
                     SRVAPEEEGVSLAREVELIDLAGVSLKRRAPHAALEAIKAYERETHGRGQMAEEAAAI
                     AIEARCNMDLDVTAAIERFDARWPESAQRERIQTACFAKP"
     gene            complement(5250..5825)
                     /locus_tag="VFV99_05840"
     CDS             complement(5250..5825)
                     /locus_tag="VFV99_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5058860.1"
                     /translation="MKNRDGSGAVAAPIPEMPAALAHAMQPEVAIPSFDEVYEAHFAF
                     VWRVLRTFGVAEAALEDAAQDVFVVVHRRLPEWEGRAQITTWLFAIARRVAQAARRKD
                     GRTDPLETEPVGAADTFAAFSRAQAAATVMSILETMDEDKRVVFALVELEQLSVPEVA
                     RMLDLNLNTAYSRLRLARHAFELAVRARVTP"
     gene            complement(5825..6790)
                     /locus_tag="VFV99_05845"
     CDS             complement(5825..6790)
                     /locus_tag="VFV99_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058861.1"
                     /translation="MRSFVLVVVTVAAIAPAAAQPATPASPGSSVAMADPTPTLQWNA
                     PPGCPDAADLRARIERRLERSLDDVVLGIEVDVAQKGSRYIARVDLRAMTVANDVRTL
                     TSTHCDEIADAVAVIVTRVASDAIARKRTAVREQDVNEKIIVEPEPRPTPRMWSVGGR
                     LGAVSGIGVIPKVGVAAELAITVRRDELMVEVAGTRWLDSYAQFHDGAPAVVNVFLDT
                     AATRVGWRPARLPLRAWAAIEFGMMDGNSTKLPGEQFDSGRWIAAGAGFGVAWQITKW
                     LRLVGSNETMLAIDRMRFRTGDGLVVYAPAPMSFRASAGIEVGWQ"
     gene            complement(6966..7787)
                     /locus_tag="VFV99_05850"
     CDS             complement(6966..7787)
                     /locus_tag="VFV99_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5058862.1"
                     /translation="MPARLHRELIGTNVSQWLLFTHGIYGSGGNWRSIARKIHERRPE
                     WGVVLVDLRQHGRSEPGDPPHTLEACAEDVRALFDELHGITVIAGHSFGGKVVLATRA
                     LAPTTLRQTWMFDASPSPRPGAETDPNNSVTRVLNLMRRLPKTWSKRDDFIAAITGAG
                     EALPLAQWLAMNLVPETSGQLALRLDLDAVGDMLQDYYAQDLWPIALDPNLPGELDVV
                     IADQSTTLSAEDRTRLAAAPPHVHVHHIDAGHWLHIEAPANVVALLTSALPTTLT"
     gene            7838..8446
                     /locus_tag="VFV99_05855"
     CDS             7838..8446
                     /locus_tag="VFV99_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058863.1"
                     /translation="MIAILSDAGFDVVHPFAAHAVARELGLSMLADPERPAGWLVANT
                     RAWWPRFLAARRADPDLAASRDPINDYVEKTCAKISGARCFFPHRQYDGAWLPFQRLA
                     VAAGFGTLSPSNLVVHPTFGPWFALRAIVLTGDTPITRVLPAAPCACAERCPVAFARA
                     REAQDSWRAWLAVRDACCVGAEHRYSEDQIEYHYTKSLALLR"
     gene            8575..9312
                     /locus_tag="VFV99_05860"
     CDS             8575..9312
                     /locus_tag="VFV99_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5058864.1"
                     /translation="MRILLATVVVAGCGFEHGVDPAGQPDSGGTDPSADASVNVPPVP
                     CKFPDGSLRLCVEFQDRKYSGSVSDTSPYQLNAIADELGEWTRNNQPAAATYWNTLVR
                     VPESPMLDITGSITFEIWVRSLYQYGTMLSNDGQYAIGMDLSGRVTCRIGNTTVTSDP
                     IGQDVWRHIACTKTDEKIAIHIDGVATKCQDASSSIPTAGTQGTKITPGFIGFIDDIR
                     IYARKLTALEICTHADKTQCQSGCTDD"
     gene            9392..>9899
                     /locus_tag="VFV99_05865"
     CDS             9392..>9899
                     /locus_tag="VFV99_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5058865.1"
                     /translation="MADVLEAFHPLVAGWFRDRFGAPTAPQLEGWPRIGAGEDVLIAA
                     PTGSGKTLAAFLACMDDLVRRGYEAPLPDHTAILYISPLKALSNDVHRNLEAPLAELA
                     ARAAIEGSAFPEIRVAVRTGDTPVGERAKMAKKPPHILVTTPESLFILLTTVKGRAAL
                     GQLRTVIVD"
BASE COUNT         1644 a         3281 c         3345 g         1629 t
ORIGIN      
        1 gtcaagatcg cgcccgatct cgcggacgct gacatcgatg cgatcgcaga cctcgcgctc
       61 gagctcgcgc tcgacggcat catcgcgacg aacacgacga tcgcgcgcac cggtctcacg
      121 acgcccgatt cgcgcgtgca agcgctcggc gccggcgggc tgtccggcgc gccgctcaag
      181 gcgcggtcac tcgacgtgct gcgccggctg cgtgcacgtg tcggcgacaa gctggtgctg
      241 atcgcggtcg gcgggatcga gaatggtgat gacgcgtggg cgcgcatccg cgccggcgcg
      301 acgctcgtgc aggcgtacac cggcttcatc tacggcgggc cgctatggcc acgccgcgtg
      361 caacgcgagc tcgcggctca cgtgcgcgca gcgggactca cgtcgatcga acaagcgatc
      421 ggcgcggatg tcccgagcgc gtcctccgct gctagttagg gaagcagaag caggtcgagc
      481 ccgccgtcgt ggcggtcgtg cccgtcgggc acatcatccc cgtgccgcac acgatgacgc
      541 agccaaggtc gatcatggtg tagctcgtgt tcgccttcag cgggttgtcc atcggggtgg
      601 tggcgaggat cacgccacac gccggcatgc caactgaacc ccccgtgtac actgccgagc
      661 acttcgcgtt atctggggcc ggggttgtgg tcgtgagctg tccctgtgcg ttggtcgttc
      721 cagaaccatc ttcgaggcaa tgcggcgtgc aatagctggt actcgcgctg ccgccaccga
      781 ggccgagcgc gatgcaatcg ggcgcgttcg tcgggcacgc cggctgaccc gagccgcact
      841 tctggccgag gccagtcagt ccgccaccgc cgccgggtgc gtccatgaac accttgctgt
      901 ccatgaatac cttcgcgtcc gggttgccgt tcccgccgcc gccgtcgtcg ccgccgcagg
      961 cgaacacagt cgttccgacg gcaacaccaa gtacgagaca cgaactaact agacgtgact
     1021 tccccatagg atggttcctc cggcgcgcac catacgaccg tttccgtcgg gaaaccaagt
     1081 gcgaatctcg cactctctgg cagatgcagc ggcgttcggt tcgctgagct acagtgcgaa
     1141 catggccatc cgcaagatcg cccagatcgg tcaccccgtc ttgcgacaaa ccgcacgcga
     1201 ggtctcgcgc gaggagctcg cctctgtggc gacgcaacag ttcatcgacg acctcgtcga
     1261 gacgatgcgc gacgccgacg gcgccggctt ggccgcgatt caggtctacg aaccagtgcg
     1321 catctgcgcg atcgaagtgc gcaacaaccc acgctacccc tacaagccgc agatcccgct
     1381 gaccatcttg gtaaacccgg tcctgacccc ggtcggcgac gagcagttcg ataactacga
     1441 gggctgcctc tcggtcccga acctacgcgg caaggtaaag cgctacgtcc acctccgggt
     1501 ccaggccttg gaccgcaatg ggaacccgat cgacgaggtc gttcacggcc tcaaggccgg
     1561 cacctaccaa cacgaggtcg accacctgaa cggcaagatc ttcgtcgacc gcgccgaccc
     1621 gagcacgttc gcgacatgga ccgagttcga acgccaccac cgcgcaaagt tcatcgagga
     1681 agcgcaagcg ctcgtcgcgc gcgtcggttc ctgaggcttg cgcgcctaat ttccgcgcgc
     1741 acgcttgccc gcaggtcggc ggcattgttt gctggcggcg atggcccgcc gtagcaagtc
     1801 cggttcggtt gcgcccaaac gcgggcgcag caagaagaag ccggccggcc cgctgatcaa
     1861 cgtgcggcac atgacccgcc gcgatatcgg cgacgcatgg gagttcctga aagtcagctt
     1921 ccgcgcggtc aaccaggaga ccgtcgagta ccagcggccg atctcgcgcc gccgcttcga
     1981 agaggtgttc gaagaggaag gcatcgaaca gctgctgttc gaggtcaacg gcgagatcgt
     2041 cgcgtacgcg gagtgcagct acgaggtcac cggttcggat aactggatca acccgcgcta
     2101 cttcgagaag cgcgacatga agccgctgtt cgtcgacgag ctcgcggtgc atccagactg
     2161 gagcggtaag ggcgtcggct cgttcgtgct cgagcagctg catcacctcg cgcgcgtgca
     2221 cggtctcaat cacctcgtgc tcgaggtcgc ggagaacaac gagcgcgcac tcaactggta
     2281 caagaagcgc tcgttcaaga agctcgacgc tgcgattttc atggcgtgtg aggtcgagat
     2341 cgagcccgag ctgctgccgc cacgggcgtt gccggtccgc ggttcgttgc cgccgccgag
     2401 ctcgggcgac gacggcgacg gcggcgatca gaccgaagat tgatacggcc tccaccgtcc
     2461 ggtgggcgtc ctacaaccgg acgcctcgtc cgagctgcgt gcccgatcga acgaggcgcg
     2521 acgcgatttc aaccggttcg acctggcacg gaccgtgctc gatgtcgtag ccagctgctt
     2581 ccgcctcatt tgtgagtcat tatgcctcta gttatgagtc agttgaccgt gcgcagtctc
     2641 gacaagcgtc tgctccatgc gatccgcgag cttgcgcgcc gggaacggat ttcccttaac
     2701 aaggccgcgc tgcgtctgct cgagcgcggc gccggtcttg cgccggccag ccaagagaat
     2761 cggatcggaa gctcgctcga tcacctcgct ggaacgtgga gtgcgctgga ggcaaaggcc
     2821 ttcgagcagg cgctcaagtc gtgtgagcag atcgacgagg caatgtggaa atgaggcggc
     2881 tcctcgacac gaacgcgtat gtcgcgtgga agcgtggcga tcggtcggta atggacatcg
     2941 tgaacggatc ctcggagctc gcgttttcga ccatcgtgat tggcgagctc ttgtacggct
     3001 tccgccacgg caaccgcttc tccaagaacg cgaacgagct cgacgagctc ttggccgacg
     3061 cgcgtgtgag cgtgttgccc gtgaccacca cgaccgcaga tcggttcggc cggatcgcag
     3121 cggtgctgcg caaggccggc acgccgatac cgacgaacga tatctggatc gccgcccacg
     3181 cattcgagag tggtgccgag ctcgtcagct tcgatgcgca cttcgagtcg gtacccgggc
     3241 tcgtctggac acaactcggc taaggggccg agcgatcaag gtgctgtaca ggcatgtggg
     3301 gcagtgagtg cggccgcgtg cgcaacaatc gtacgcgcac ggcgcgcgcc ccgacgcata
     3361 tgatggtggg gatgggaggc tggccgactg catgtgcgat gctcgcgctc gcgggatgct
     3421 cgttcgagca cggcgtcgcc cccgacgagc tcggcagcga cggcgggtat caagatgctg
     3481 tcggcaccgg gctggatggt gcgatcggat cgagttgcta cggaccggcg gccggcgcgt
     3541 ggcgcgtgtg cctgggcagc gtgccgacgg gaggcgtgat gctgccgcca accctcgaca
     3601 cgacgacgag catgttgtgc ctgcaagcgc agccggcgga atggacgacg agcggtcagc
     3661 cgcccgcgtg cttcgtcgtc ggcgacgcga tccagacgtc gaagaccgtg gtgacgggta
     3721 gtcggccgct cgtgctcctg tcgtccggca cgatcacgat cggcagcctc gatctcgcga
     3781 gccacgcgga ttcgagcgcc accggccccg gcgccgatcc gtcagtatgc aaaccgttcg
     3841 cggcgaatcc gcagacgggc gatggcaacg gaggtggcgc cgccggcagc ttcatgacca
     3901 agggtggcga cggtggcgac ggccacgacg gagatgagaa aggcggccgc gcggcgacgg
     3961 cggatggcac ggcaccggcg gtgctgcgcg gtggttgccg cggtcagaaa ggcggcgggt
     4021 tctcgggcgg ggcgggtggc cacggcggcg gcgctgcata tctggttgct gcgacctcga
     4081 tcatggtgac cggcacgatc aacgcgagcg gcgccggtgg ctcggctggc aacaacaaca
     4141 agcccggcgg cggcggtgcc ggttcgggcg gcatgatcgt gttgtacgcg ccgctcatcg
     4201 gagtgaccgg caccgtgttc gccaatggcg gtggcggtgg cggcggcggc gacggcgacg
     4261 gttcgcctgg cgatgatcca ttgttcgcgg gcgtcactgc aggcggagga agtcctgccg
     4321 gtggcgacgg ctatgcgctt tcggtcaacg cgaccagtgg tggtagagac aaggacggcg
     4381 gcggtggcgg aggtggcggc gccggctaca tccgatcgaa tctgacgatc ggcgcgacct
     4441 cgccagccgc tgacatcgtg ccgtagctca tcgcgatcaa ggcttggcga agcaggcggt
     4501 ctggattcgc tcgcgctgcg ccgactcggg ccacctcgcg tcgaaccgct cgatcgcggc
     4561 ggtcacgtcg aggtccatgt tgcagcgcgc ctcgatcgcg atcgcagcgg cttcctcggc
     4621 catctgaccg cgcccgtgcg tctcgcgttc gtacgctttg atcgcttcga gtgccgcgtg
     4681 cggggcacgc cgcttcaacg agacacccgc gagatcgatc agctcgactt cgcgcgcgag
     4741 cgacactccc tcttcttcag gagcgacgcg agagccacgc gccttgctcg gatgggccga
     4801 cgcagctccg tcgtcgggct ctcgagattc ggctccgtcc tcgaccgcgt cgctggtcga
     4861 ctgcacgtgc gcgtgatgct ccggttgcga tacgaccggc tgcgagcggt ctggctgcaa
     4921 tggcggttcc gcgggtgcga cgacgcgaac ctcgctgcga acctcgtcgc ccgcgcgcgg
     4981 ggcgacatcg acacggggcg cttcaccgcc gcgattcggc cgcgctacca cgacgaccgt
     5041 ggcgaccgcg ccgacggcga gcaatgcggc accgagcttg agcagcagcg acgacgagcc
     5101 gacctttgct gcgggaattg ccgccgcggc accggtggcg gccgttgcgc cgaccgcggc
     5161 agcgaccttc gcgcgcacgc gtgcggcgac ggcgtcgtcg ggcgcgaggc cttcccgcgc
     5221 ggcggcgatg agggagcgag acagctcggt catggcgtca cccgggcgcg cacggcgagc
     5281 tcgaaggcgt gacgcgcgag gcgcaatcgc gagtatgccg tgttgaggtt gaggtccagc
     5341 atgcgagcga cctcgggaac cgagagttgc tcgagctcga cgagtgcgaa cacgacgcgc
     5401 ttgtcttcat ccatcgtctc gaggatcgac atgacagtcg cggcggcttg cgcgcgcgag
     5461 aacgcggcga acgtatcggc ggcgccgacc ggttcggttt ctagcggatc ggtgcggccg
     5521 tctttgcgcc gcgcggcctg cgcgacgcga cgagcgatcg cgaacagcca tgtcgtgatc
     5581 tgcgcgcgcc cttcccactc gggaaggcga cgatgcacga cgacgaacac gtcctgcgcc
     5641 gcatcttcga gcgcagcctc ggcgacgccg aacgtgcgca gcacccgcca cacgaatgcg
     5701 aagtgcgcct cgtagacttc atcgaacgat gggatcgcga cctcgggttg catcgcgtgc
     5761 gcgagcgcag caggcatctc tggaataggt gcagcgactg cccccgatcc gtcacggttc
     5821 ttcactactg ccaccccact tcgatgccgg ccgacgcgcg gaacgacatc ggtgccggcg
     5881 cgtagacgac gagaccgtcg ccggtgcgaa agcgcatgcg atcgatcgcg agcatcgtct
     5941 cgttgctacc gacgaggcgg agccacttcg tgatctgcca cgcgacgccg aagcccgcgc
     6001 ccgctgcgat ccagcggccc gagtcgaact gctcgcccgg cagcttcgtg ctgttgccat
     6061 ccatcatgcc gaattctatc gctgcccacg cacgcaacgg cagacgagcc gggcgccagc
     6121 cgacgcgcgt cgctgcggtg tcgagaaaca cgttgacgac tgcgggcgcg ccgtcgtgga
     6181 actgcgcgta gctgtcgagc cagcgtgtac cggcgacctc gaccatcagc tcgtcgcgac
     6241 gtacggtgat cgcgagctcg gcagcgacac caaccttcgg gatgacgccg atgccactga
     6301 ccgcgccgag gcggccaccg actgaccaca tgcgaggtgt cggccgtggc tccggctcga
     6361 cgatgatctt ctcgttcaca tcttgttcgc gcaccgcggt gcgcttgcgc gcgatcgcat
     6421 cgctcgcgac gcgagtcacg atcaccgcga cagcatccgc gatctcgtcg cagtgcgtgc
     6481 tcgtcagcgt gcggacatcg ttcgcgaccg tcatcgctcg cagatccacg cgcgcgatgt
     6541 agcgactgcc tttctgcgcg acatcgactt cgatcccgag caccacatcg tcgagcgagc
     6601 gctcgaggcg gcgctcgatg cgggcgcgca gatccgctgc gtcgggacaa ccaggcggcg
     6661 cgttccactg cagggtgggc gtcggatcgg ccatggcgac cgagctaccc ggcgaggccg
     6721 gtgtggcagg ctgcgcggca gcaggtgcga tggcggcgac cgtgacgaca acgaggacga
     6781 acgaccgcat gagacggcga cagccgctcg gttgccgagt tgcctgacaa cgtctgccgc
     6841 gagtgcggag cgtcggctgg ggacatgggt cgacagtgcc ggccacgggt gcagtggtta
     6901 gtgccggaac cgggccagat ccgtcacggc ctaacaccgc gacttcgcgt catatagctg
     6961 ccgactcagg tcaacgtcgt tggcagcgct gaggtcagca gagccaccac gttggcgggg
     7021 gcctcgatgt gaagccagtg gccggcatcg atgtgatgta cgtggacgtg cggcggggcg
     7081 gcggcgagcc gggtgcggtc ctcggcggag agcgtcgtcg actggtccgc gatcacgacg
     7141 tcgagctcgc ctggcaaatt cggatcgagc gcgatcggcc acagatcctg cgcgtagtag
     7201 tcctgcagca tgtcgccgac cgcatcgaga tcgaggcgca gcgcgagctg accactcgtc
     7261 tccggcacga ggttcatcgc gagccattgc gcgagcggca gcgcttcacc ggcgccggtg
     7321 atcgcggcga tgaaatcatc gcgtttgctc cacgtcttcg gcagccgtcg catcagattc
     7381 agcacgcgcg tcaccgagtt gttcggatcg gtctcggcgc ccggacgcgg gctcgggctc
     7441 gcatcgaaca tccaggtctg gcgcagcgtc gtcggtgcga gcgcgcgcgt cgcgagcacg
     7501 accttgccgc cgaagctgtg ccccgcgatc accgtgatgc cgtgcagctc gtcgaacaat
     7561 gcgcgcacgt cctcggcgca ggcttcgagt gtgtgcggcg ggtcgccggg ctcggaccgg
     7621 ccatgctggc gcagatcgac gagcacgacg ccccactccg gccgacgctc gtgaatcttg
     7681 cgcgcgatgc tgcgccagtt gccgcccgag ccgtagatgc cgtgcgtgaa caaaagccac
     7741 tgactgacgt tggttccgat cagctcgcga tgtagacgcg ccggcaccgc gtgatgatgc
     7801 cattgcctcg caatcgtggc accgtgacgg cgtggacatg atcgcgatcc tcagtgacgc
     7861 cggcttcgat gtcgtccatc cgttcgctgc gcatgcggtc gcgcgtgagc ttggcctcag
     7921 catgctcgct gatccagagc ggccggcggg ctggcttgtc gccaacacgc gtgcctggtg
     7981 gccgcggttt ctcgccgcgc ggcgcgcgga tccggacctc gctgcgagtc gcgatccgat
     8041 caacgactac gtcgagaaga catgcgcgaa gatttccggc gcgcgttgct tctttccgca
     8101 tcgccagtac gacggtgcgt ggctgccgtt tcaacggctc gccgtcgccg cgggattcgg
     8161 cacgctctcg ccgagtaatc tggtcgttca cccgacgttc gggccgtggt tcgcgctgcg
     8221 tgcgatcgtc ctgaccggcg acacgccgat cacgcgcgtg ttacctgccg ccccatgcgc
     8281 ctgcgccgag cgctgtcccg ttgcgttcgc acgtgcacgt gaagcgcaag attcatggcg
     8341 tgcgtggctc gcggtccgcg acgcgtgctg cgtcggcgcc gaacaccgct acagcgaaga
     8401 tcaaatcgag taccactaca cgaagagcct cgcgctcctt cgctagcgtg cgcagggctc
     8461 gtgcagagga cgcgggttcc caacccagtg gggactccac tactccagcg cggttcgagt
     8521 ggtcgtcgga tcgagacctt agccacggcc tgacgcttgc actctccgac gggcgtgagg
     8581 atcctgctcg cgacagtggt ggtcgccggc tgcggcttcg agcacggggt ggacccggcc
     8641 gggcagcccg acagtggagg caccgatcca agcgccgatg cgtcggtgaa cgtcccgccg
     8701 gttccgtgca agttccccga cggctcgctg cgcctctgcg tcgagttcca ggatcgcaag
     8761 tactcgggca gcgtctcgga tacgtcgccc tatcagctca acgcgattgc cgacgagctc
     8821 ggcgagtgga cccgtaacaa ccagccggca gccgcgacct actggaacac gctcgtacgc
     8881 gtcccagaat ctccgatgct ggacatcacg ggctcgatca cgttcgagat ctgggtgcgg
     8941 tccctgtatc agtacggcac gatgctgtcg aacgatggcc agtacgcgat cggcatggat
     9001 ctgtcgggcc gtgtgacctg ccggatcggc aacacgacgg tcaccagcga cccgatcggc
     9061 caggacgtgt ggcgccacat cgcgtgcacg aagaccgacg agaagatcgc gatccacatc
     9121 gacggcgtcg cgacgaaatg tcaagacgcg tcgagctcca tcccgaccgc cgggacgcag
     9181 ggcaccaaga tcacgcccgg cttcatcggc ttcatcgatg acatccgcat ctacgcgcga
     9241 aagctgaccg cgctggaaat ctgtacgcac gcagacaaga cgcagtgcca atccggctgc
     9301 accgacgact gagggatctc tccctccgga tccgccaatc gtcgtcggtg ggattccgtt
     9361 tgccgtcgac tgcggtacgc tgattggccg catggcagac gtgctcgaag cattccaccc
     9421 gctcgtcgcg ggttggtttc gcgatcgctt cggcgcgccg accgcacccc agctcgaagg
     9481 ctggccgcgg atcggtgccg gcgaagacgt gttgatcgcg gcgcccacgg gctcgggcaa
     9541 gacgctcgcc gcgttcctcg cgtgcatgga cgatctagtc cggcgcggat acgaggcgcc
     9601 actgccggat cacaccgcga tcctgtacat ctcgccgctc aaggcgctgt cgaacgacgt
     9661 ccatcgcaac ctcgaagctc cgctcgccga gctggcggcg cgggcagcga tcgagggctc
     9721 ggcgtttccg gagattcgcg tcgcggtgcg taccggtgac acaccggtcg gcgagcgcgc
     9781 gaagatggcg aagaagccgc cgcatattct ggtcacgacg ccggagtcgc tcttcatcct
     9841 gctgacgacc gtgaagggcc gcgcagcgct cggtcagctg cgcacggtga tcgtcgacg
//
LOCUS       DASUFX010000148         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1690693, whole genome shotgun sequence.
ACCESSION   DASUFX010000148 DASUFX010000000
VERSION     DASUFX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690693"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFV99_05870"
     CDS             <1..945
                     /locus_tag="VFV99_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058866.1"
                     /translation="AGLPIHVHRGGQGGEDDVGCEKAALRVYFNIGMCAAECMVGHLH
                     NGPGGSQTEIDDAECSAACPEYVRAKSEVSGMCAFMATNKVPKLEDAPGGAAAINTAV
                     LDRGKKVFKLACAGCHSNGGPPGHEVYSNDLINPMILVGTNSCRARSTNWKEGQIWGQ
                     FSSDEYKARATGGPGYYRNVPLLGVWATAPFFHNNRLGSYNGDPSVAGRLAAYEDAMD
                     QLLNPAHRNFGASVQRTTVQITIPTAGGPVTLPAGTPIGAFANVNPATGQNLCPVPGT
                     EALGALAPPDVVENQGHYFGAALSDSDKYALKEWLKTK"
     gene            1164..2180
                     /locus_tag="VFV99_05875"
     CDS             1164..2180
                     /locus_tag="VFV99_05875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058867.1"
                     /translation="MNLAAGTIFAERYEILATQGRGGMGYVYRARHLGLAKNVALKVL
                     ATSKLDYEERFAREARAVARLDHPGCVRVLDHGRADGAQYIAMELIDGETLGTVLQRG
                     RFTKAHALDIARQLLHALAHAHAQGVIHRDIKPENIMLTPLRHQTGGAGLSAGPGGKR
                     AVLIDFGLATVRNELPLTGAGMCVGSPSYVAPERLLGRPHDTRTDLYAVGVVLYEMIA
                     GVKPFNGTSPEHTMRLALHRPPRPLRAFRRDIPAVLDRVIRRALQKDPGRRFQDAEEM
                     LLALNDVPTDEEIAERIAAAEREEQDEAAATIAIARLEIREPSLLSRIWTWLRYGGWR
                     WRHG"
     gene            2196..>2651
                     /locus_tag="VFV99_05880"
     CDS             2196..>2651
                     /locus_tag="VFV99_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5058868.1"
                     /translation="MPTRRDVLAGSLAGGAAALLGPTLATAKTAPPPAPTKPGAHEVK
                     PLPFDAAKLKGLSAAMLTSHHDNNYAGAVKNLNKVELDIEKINKDTPGYLVSSLRERE
                     LTFTNSKILHEHYFGNLGGDGKLAGAIDKRLAAQFGSTARWEELFRASAM"
BASE COUNT          468 a          888 c          880 g          415 t
ORIGIN      
        1 gcgggtctgc cgatccacgt ccaccgtggc ggccaaggcg gcgaggacga cgtgggctgc
       61 gagaaggcag cactgcgcgt ctacttcaac atcggtatgt gcgcggccga gtgcatggtc
      121 ggccacctcc acaacggccc cggtggctcg caaaccgaga tcgacgacgc cgagtgctcg
      181 gcggcgtgcc ccgagtacgt gcgtgcgaag tctgaagtca gcggcatgtg cgcgttcatg
      241 gcgaccaaca aggtgccgaa gctcgaggac gctccgggcg gcgcggctgc catcaacacc
      301 gcggttctcg atcgcggcaa gaaggtcttc aagctcgcgt gcgccggctg ccactcgaac
      361 ggcggtcctc caggtcacga ggtctactcg aacgacctga tcaacccgat gatcctcgtc
      421 ggcacgaact cgtgccgcgc gcgcagcacg aactggaagg aaggccagat ctggggccag
      481 ttctcgtcgg acgagtacaa ggcacgcgcc acgggtggcc cgggctacta ccgcaacgtg
      541 ccgctgctcg gcgtgtgggc gaccgctccg ttcttccaca acaaccggct gggtagctac
      601 aacggcgacc cgtcggtggc cggccgcctc gccgcgtacg aggacgcgat ggatcagctg
      661 ctcaacccgg cgcatcgcaa cttcggcgcg tcggtgcagc gcacgacggt ccagatcacg
      721 atcccgaccg cgggcggtcc ggtgacgctg cctgccggta cgccgatcgg tgcgttcgcg
      781 aacgtcaacc cggcaacagg tcagaacctg tgcccggtgc cgggtacgga ggcactcggc
      841 gctctcgcgc cccccgacgt ggtggagaac cagggccact acttcggcgc ggcgctgtct
      901 gactcggaca agtacgccct caaggagtgg ttgaagacca agtagccctc accgacttcc
      961 agtagtccga cgacgcgcga tggttgaccc catcgcgcgt tgtcgtttcg gcttcgttaa
     1021 gcgtgacacg gcgacgtgga ttcgcttgca ctttctcggc gacgtgaagc tcgtcggcca
     1081 caggcaccgt gaacagttct cctcgatgtg cgtccaagga ggcggataca cgagaggcat
     1141 gtaccttgcg tgcgacttcg atagtgaacc tcgcggcggg aacgatcttc gccgaacgct
     1201 acgaaatcct cgcgacccaa ggccgcggcg gcatgggcta cgtatatcgc gcgaggcacc
     1261 tcgggctcgc caaaaacgtg gcgctcaagg tccttgcgac gagcaagctc gactacgagg
     1321 agcggttcgc ccgcgaggcg cgcgcagtcg cacgcctcga ccatccgggc tgtgtgcgcg
     1381 tgctcgacca cggccgtgcg gacggcgcgc aatacatcgc gatggagctc atcgacggtg
     1441 agacgctcgg tacggtgctg cagcgcggtc gcttcacgaa ggcccatgct ctcgacatcg
     1501 cgcgacagct gctgcacgcg ctcgcgcatg cgcacgcgca aggcgtcatt catcgcgaca
     1561 tcaaacccga aaacatcatg ctcacgccgc tgcgtcatca gaccgggggg gccggattgt
     1621 ccgccggccc tggcggcaag cgcgcggtgc tcatcgactt cggactcgcg accgtgcgca
     1681 acgagctgcc gctcaccggc gcgggcatgt gcgtcgggtc gccatcgtac gtcgctcccg
     1741 agcggctgct cggccggcct cacgacacgc gtaccgacct ctacgcggtt ggcgtcgtgc
     1801 tgtacgagat gattgccggc gtgaagccgt tcaacggcac gtcgccggag cacacgatgc
     1861 gactcgcact gcatcggccg ccgcgcccgc ttcgcgcatt tcgccgcgac atcccggccg
     1921 tgctcgaccg cgtgatccgc cgtgcgctgc agaaggatcc ggggcgtcgc tttcaggacg
     1981 ccgaggagat gctgctcgcg ctcaacgacg ttcccaccga cgaggaaatt gccgaacgca
     2041 tcgccgcggc ggagcgcgag gagcaagacg aagcggcggc gaccatcgcg atcgcgcgcc
     2101 tcgagattcg cgagccgtcg ctgttgtcac gaatctggac ctggttgcgc tacggcggct
     2161 ggcgctggcg acacggctag cgttacatct cgggcatgcc gacacgacgt gatgtgctcg
     2221 caggctcatt ggcgggcggg gctgctgctc tgctcggtcc cacgctcgcg actgcgaaga
     2281 ccgcgccgcc tccggctccg acgaagccgg gcgcgcacga ggtcaagccg ctgccgttcg
     2341 atgcagcgaa gctcaagggc ctatcggcgg cgatgctgac gtcgcaccac gacaacaact
     2401 acgccggtgc ggtgaagaat ctgaacaagg tcgagctcga catcgagaag atcaacaagg
     2461 acacgccggg ctatctcgtc tcgagcctgc gcgagcgcga gctgacgttt acgaactcga
     2521 agattctgca cgagcattac ttcggcaacc tcggcggcga cggcaagctc gcgggcgcga
     2581 tcgacaagcg actggccgcg cagttcggct cgacggcacg ctgggaggag ctgttccgcg
     2641 cgagcgcgat g
//
LOCUS       DASUFX010000149        10344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1829837, whole genome shotgun sequence.
ACCESSION   DASUFX010000149 DASUFX010000000
VERSION     DASUFX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829837"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1278
                     /locus_tag="VFV99_05885"
     CDS             241..1278
                     /locus_tag="VFV99_05885"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5058869.1"
                     /translation="MELRPIASYAQAVRPTLPDAVFEPARSRLLWLPVHVTVIAGIAW
                     ALAAGHVPAPLWPVASLVIGVCMSAVAFLGHETLHGAVVRGRLAIRVVGWICLLPFTC
                     SPTLWTAWHNRVHHNQCAKPGADPDMYPTLSEYETQPAMRIMADYFGLGGRRLSSVFS
                     LLLGFTGQSQQMLWEARKHGFLDGPRYMRAIGELVLGVGFWTAVALLIGFVPFAFVYL
                     LPLIVANVVVMSFIMTNHNLSSLTPTVNDPLVNSLSVTLPRPLEWLSLDFGYHVEHHV
                     FPTMSARHGRRVREALRAQFPDHYQSMPLPRALGQLWRTGRVYQDNTTLIDPRTGRTA
                     PTLQPRRDASL"
     gene            complement(1270..1968)
                     /locus_tag="VFV99_05890"
     CDS             complement(1270..1968)
                     /locus_tag="VFV99_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058870.1"
                     /translation="MLCWRAGVGVCAALLASCSFSHGLGIDDASPADDVASDSGNHPS
                     DTATPSDTSSPLIDAPPPPLNLIASSAGATLVSFTSEYCTQNNPPLLCQNGYWNRTNL
                     NDGQHANGDNTTAYRNAWASTNKQNNNHEEFELAFANNSNALIDRFVIQNWGRGNGVT
                     LYYSTHARIYGRDPQTMNWAMLVDTALATNETPQTFVLSDQVVVDRVRLSITDGLRTD
                     YWELGEFEAWGWLQ"
     gene            2087..2740
                     /locus_tag="VFV99_05895"
     CDS             2087..2740
                     /locus_tag="VFV99_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058871.1"
                     /translation="MRSVVLVTVAFVVTGCGRLNFDPLGRSSGGDDAGNDAGDDAREF
                     ADGPGSGGTFTYTATIAECIDPTAPDPAFCRTTNGQTELPVDLDDSNLHVPFYSYLRF
                     DFDASIAGKEVAAVVLRAVISDGAQSDGPDSGEIWRVSTFTLASLSTQAPTQQGGAAL
                     AASQGPVVQLQEVDWLLPTTVIDSSSLCLGMIPVSAAGVNYWNLQGATPPTLRVELR"
     gene            2945..3793
                     /locus_tag="VFV99_05900"
     CDS             2945..3793
                     /locus_tag="VFV99_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5058872.1"
                     /translation="MRLLCLVLVAGCYAPTYAPGGRCTTTCPGDLVCVDSVCVVPGTV
                     VDHDAAVMIDAPGNVIMIDAPPPPPPIDAPPPDPTLIAHWKFDDNPANGTALDSTGNG
                     HNGTCAGAQCPTLVTTGVDGGAYRFDPAVPQYLVVPDSTAFRGVFTIAMWMYTDNTTK
                     QIAAMSKPVGTGTGNSWQLEDLTDDTVSFSGGSVHSLVSPAAVPQMTWAHIAGTWDGT
                     TKRLYINGVMVASTASQITYDTHNIYLGADENNGTLALPFDGELDDLRVYNRVLSTSE
                     IAALAQ"
     gene            complement(3794..5122)
                     /locus_tag="VFV99_05905"
     CDS             complement(3794..5122)
                     /locus_tag="VFV99_05905"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5058873.1"
                     /translation="MVRHLTSVPDDLDELTGGLRRMWSTASLSSKLGAKAASRMFFRR
                     KDKADDVDIDASLASTDAAIAAARKLVTRMGNLKGLVMKAGQIASYMPGSLPPAAQEV
                     LAELQAQSTPMAFHRIADVLRTELGAPASELFETIEERPFAAASIGQVHRATHRGQAV
                     AVKVQYPGIEDAIRSDLKMVAVIARLSSIGSPLDGAGLAGELRDRLLEECDYEREATS
                     QTLFARLLASVDGAGVPAVIGERSSRRVLTTVLGDGAPLAQSDPAARDRAGQVIFRAC
                     FELMWRRCIYNADPHPGNYLVAADGTVTFLDFGCVRRFEPAMIEIWREMARAVIVGDR
                     EGFATRFRVLGFVGKEKGFDWDYQWNAMRFLYTPFLERGFRHNPAFVAKSFGVLMFDN
                     PNRFRLAMPPEWLFLNRLQWGLNAVLAQLGATGDWGGIIDEILASKIEPA"
     gene            5164..6174
                     /locus_tag="VFV99_05910"
     CDS             5164..6174
                     /locus_tag="VFV99_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5058874.1"
                     /translation="MKGATVLTVSSRALVDACDALGMDTAALLAATGLTRAEVNNPDG
                     RIPVEKMTALWREAHARAGDPDLALHAAEALPFGAYAVIDFLARTSATIGAALERISR
                     YFPLINSVVELPIERGSDYVRLDMVDRRGPGKLPRAYAEYVLAAVVLRTRGAVGVAFP
                     LVRVELAYEAPALVCEHERIFGCPVVFGTERTGIVLAHSVADKSLERGDSGLAAVLER
                     HAQLLISQLPPVSDSISEVRATIKGELEGGDPSLDHVARKLGTSRRSLQRRLADENLT
                     YAQVLDEVRSTMARAYLGRPELSIAEVAYLLGFSEQSSFTRAFRRWTGTSPAEFRRTS
                     TS"
     gene            complement(6075..6755)
                     /locus_tag="VFV99_05915"
     CDS             complement(6075..6755)
                     /locus_tag="VFV99_05915"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX5058875.1"
                     /translation="MRRLRLGLVVFLSIGVGLLAPLPWLLGLVEPAANQPPLVEHLLA
                     RYTVAQLVFVVHVVGGGIALLAGPWQLMPRLRARRPRLHRATGYIYVSAVLLGGSAGF
                     VMGPNAWGGLVAQVGFTALAIAWLTTTAVGLHRILSGDRDGHRAWITRSYALAFAAVS
                     LRLQLPLLGLLGVQGAIAYATVAWSCWVPNLVVAQLVLVRRNSAGDVPVQRRNARVNE
                     LCSENPSR"
     gene            complement(6948..7259)
                     /locus_tag="VFV99_05920"
     CDS             complement(6948..7259)
                     /locus_tag="VFV99_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058876.1"
                     /translation="MRKYLLGAALALASIPGCAKDKQADKTGSAATATDNLPTMTMDD
                     VEKALADKQAVAVDCNGDRTRKKYGVLPGAILTSEEDFPASELPPDKATKLVFYCTDS
                     G"
     gene            complement(7305..7727)
                     /locus_tag="VFV99_05925"
     CDS             complement(7305..7727)
                     /locus_tag="VFV99_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058877.1"
                     /translation="MLFERAVVMGGSMAGLLAARVLAEYASEVVIVDRDQFPVQPEFR
                     SGVPQARHAHALLRYPGTEGERKFAHRLAYAYMDRLFIRSAREPAVRLALIHVFGMTK
                     PPTHLFRLPLAFRALTTSIPPTLVRPQHALAQKPLAPG"
     gene            complement(7758..8165)
                     /locus_tag="VFV99_05930"
     CDS             complement(7758..8165)
                     /locus_tag="VFV99_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058878.1"
                     /translation="MPQPSKRAVWLLAGLCVAVALLVVLTLQGLRSSTEPAKPSNAAV
                     PTTPSRATATPGTTPAVPDETPPWKTTPQTTGTLPPGTVPAWQVNPPPPDPSTPPPPA
                     PREPANPALHRPAMDNPGGVNGARPERKVPGVE"
     gene            complement(8185..>10344)
                     /locus_tag="VFV99_05935"
     CDS             complement(8185..>10344)
                     /locus_tag="VFV99_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058879.1"
                     /translation="FEELHETSAAQAGVLEGKAMEWQVMARGVEVGTLTGHHTPEQNE
                     EELIELVDYVLPTIWMSVDNAISFTEVLQFKRTLEDKVVERTAQLAEANEQLVEAKAA
                     RDRFFANINHEIRTPLTLIQLASDAIMRSGEALSGNTRRNLDEINASTRRLLHLVNSL
                     LLLAAGDEGKLRVRPGPCDVATCLRRLTRNWVTAAQKGDLEITFVGPDECAATIDEAA
                     LETIVGNFISNAVKFSPPGGRITVSLSAKEHAVTVNVRDTGPGIEPEFIPKLFGRFER
                     AATAVGRGVRGTGIGLALSKELVELQHGTIRVDRHDDPSGTSFEVTLPRFQTGSAILP
                     QEEPLQRPSALEEDPTARNVRAASRETAEPREPEATIVLAEDDLGLAQHITDLLSGRY
                     RVLAATNGKAALALAKEHIPDLLVTDLEMPEMNGLDLTREFLALQGTALSPVLIVSAH
                     AGLGERLAGFEAGAVDYVLKPFSAEELLARIRSQLAIRKMALKLHESEKLAALGMLSA
                     GLAHELRNPANALVNALQPMMANLPELQKGSNTAEAMLAEIAVDAANQIRERCSRILD
                     FSRSEQVVRRPEDVRALIARARRVLTTQLANVEIREQIELDEPLYCAGPLIEQIVINL
                     LDNAAFAAGPQGWVQLSVRSEGALAVIEVGDSGPGVPPQLLERIFDPFFTTKPVGKGT
                     GLGLTISRRIAKNHGGDLRVVRQGNGSMFRLELPRGA"
BASE COUNT         1693 a         3386 c         3406 g         1859 t
ORIGIN      
        1 atgtcgaacc acgacacgcc cgcgacgagc ggaaggaagc agccgatgag accgaggacg
       61 ccaaacgcgg agatggagcg cttcatcatg gggtagaacc ccggcgtgcg aggtagtcgc
      121 ataaattctt gtaaccgccc gccaccacga cagattttgc ctgaggagca gatccttgca
      181 gtacgatgac cggatggtgc gctccggagc tgcatcgcgg gtctacaagc cgacgtgagc
      241 atggagttgc ggccaatcgc gagctacgcg caggcggtgc gaccgacgtt gcccgacgcg
      301 gtgttcgagc ccgcgcgttc gcgcctattg tggctgcccg tgcacgtgac cgtgatcgcg
      361 gggatcgcgt gggcgctcgc ggcgggtcat gttccggcgc cgctgtggcc ggtcgcttcg
      421 ctcgtaatcg gcgtgtgcat gtcggccgtg gcctttctcg gccacgagac gctgcacggc
      481 gctgtcgtgc gcggacgact cgcgatccgc gtggtcggtt ggatctgcct gctgccgttc
      541 acatgctcac cgacgctgtg gaccgcctgg cacaaccgcg tccatcacaa tcaatgcgcg
      601 aagcctggcg cggatccgga catgtacccg acgctgagcg agtacgagac gcagccggcg
      661 atgcgcatca tggccgatta cttcggtctc ggcggccggc ggctgagcag cgtgtttagc
      721 ttgctgctcg gcttcaccgg ccagagtcag cagatgctgt gggaggcacg caagcacggc
      781 tttctcgatg gcccgcggta catgcgcgcg atcggtgagc tcgtgctcgg cgtcgggttt
      841 tggaccgccg tcgcgctgct gatcggcttc gtgccgttcg cattcgtgta tctgctgccg
      901 ctgatcgtcg cgaacgtggt cgtgatgagc ttcatcatga cgaatcacaa cctcagctcg
      961 ctgacgccga cggtgaacga tccgctcgtc aactcgctct cggtcacgtt gccgcgcccg
     1021 ctcgaatggc tctcgctcga cttcggctat cacgtcgagc atcacgtgtt tccgacgatg
     1081 agcgcgcggc acggtcgtcg cgtgcgcgag gcactgcgcg cacagttccc cgatcactat
     1141 caatcgatgc cgctgccccg ggcgcttggc cagctctggc gcaccggccg cgtatatcag
     1201 gacaacacga cgctcatcga tccgcgcacc ggccgtaccg cgccgacgtt gcagccgcgg
     1261 cgcgacgcat cactgtagcc agccccaggc ctcgaattcg ccgagctccc agtagtcggt
     1321 gcgcaggcca tcggtgatcg acaggcgaac gcgatcgacg accacttgat cgctgagcac
     1381 gaacgtctgc ggcgtctcgt tcgtcgcgag cgcggtatcg acgagcatcg cccagttcat
     1441 cgtctgcgga tcgcggccgt agatccgcgc gtgcgtcgag tagtacagcg tgacgccgtt
     1501 gccgcggccc cagttctgga tcacgaagcg atcgatcaga gcgttgctgt tgttcgcgaa
     1561 cgcgagctcg aactcttcgt ggttgttgtt ctgcttgttc gtcgacgccc acgcgttgcg
     1621 atacgccgtc gtgttgtcgc cgttcgcgtg ctggccgtca ttgaggttcg tgcggttcca
     1681 gtagccgttc tggcacaaca gtggtggatt gttctgcgtg cagtactcgc tggtgaatga
     1741 cacgagcgtc gcgcctgcgc tcgacgcgat gaggttgagc ggaggtggtg gcgcgtcgat
     1801 cagcggtgac gatgtgtcgc ttggcgtcgc ggtgtcgctc ggatgattcc cgctatcgct
     1861 cgcgacgtcg tcggccggcg atgcgtcgtc gatcccgagg ccgtgcgaaa acgagcaact
     1921 cgcaagtagg gcagcacaca cgccaacacc tgcacgccag cacagcacgc gtccattttg
     1981 ccacgacagc ccgcaacgct ctgatcgaga aattcagtcg tgaaacgtcg gaactggcga
     2041 gggatttcgc gcgcagcgac gtaccgcgtc ctgataagct ggagcgatgc ggtcggttgt
     2101 gctcgtcacg gtcgcgtttg tcgtgacggg ctgcgggcgc ctcaacttcg atccgctcgg
     2161 cagatcatcc ggtggcgatg acgcaggcaa tgacgcaggc gatgacgcgc gcgaattcgc
     2221 tgacggccca gggagcggcg gcacgttcac gtacaccgcc acgatcgccg agtgcatcga
     2281 tccgacggcg cccgatccgg cgttctgtcg tacgaccaac ggtcaaaccg agctgccggt
     2341 cgatctcgac gactcgaacc ttcacgttcc gttctactcg tatctgcgct tcgacttcga
     2401 tgcgtcgatc gcgggcaagg aagtggcggc ggtcgtgctt cgtgcggtga tcagcgacgg
     2461 cgcgcaatcc gacggtcccg actctggcga gatctggcgc gtctcgacat tcacgctcgc
     2521 cagtctctcg acgcaggcgc cgacgcagca gggcggcgca gcgctcgcgg cgtcgcaagg
     2581 tcccgtcgtt cagctccaag aggtcgactg gttgctgccg acgaccgtca tcgacagcag
     2641 ctcgctgtgc ctcggcatga tcccggtctc cgcggccggc gtgaactact ggaacctgca
     2701 gggcgcgacc ccgccgacgc tgcgggtcga gctgcgctag ctcggtcggg acggacgtct
     2761 acatcatggc gcgtgcttac ccgagctact ctggggtaac gctgtccgcg atttgagcaa
     2821 tctgcgcagc gcgagtacag aacgtaggct gcactgcaat cgcttcaacc ggtcgccagc
     2881 cccgcgtgtc gggtgtcagc ggattcgagt tctacactcg cgcaagcctg gatagacttg
     2941 gaacgtgcgg ctgttgtgcc tcgtgctcgt tgctggatgc tacgcgccga cgtatgcgcc
     3001 gggcggccgt tgcacgacca catgtccggg tgatctcgtc tgtgtcgata gcgtgtgcgt
     3061 ggtcccggga acggtggtcg atcacgacgc cgcggtcatg atcgatgccc ccggtaacgt
     3121 gatcatgatc gacgcgccgc caccaccacc gccgatcgac gcgccgccgc cggatccgac
     3181 actgatcgcg cactggaagt tcgacgacaa cccggcgaac ggaaccgcgc tcgacagcac
     3241 cggcaacggc cacaacggca cgtgcgcggg cgcccagtgt cctacgctcg ttacgactgg
     3301 cgtcgacggt ggtgcctacc ggttcgatcc ggcagtgccg cagtacctcg tcgtccccga
     3361 cagcacggcg ttccgcggtg tgttcacgat cgcgatgtgg atgtacacgg acaacacgac
     3421 gaagcagatc gccgcgatgt ccaagcccgt cggtacgggc accggtaact cgtggcagct
     3481 cgaggacctc accgacgata ccgtgtcgtt ctcgggtggc tccgtgcatt cgctcgtgag
     3541 cccggcggcg gtgccgcaga tgacgtgggc acacatcgcc ggtacgtggg acggcacgac
     3601 gaagcggctc tatatcaacg gcgtgatggt ggcgtcgacc gcgtcgcaaa tcacgtacga
     3661 cacgcacaac atctacctcg gtgccgacga gaacaacggg acgctcgcgc tgccgttcga
     3721 cggcgagctc gacgatctcc gcgtctacaa tcgcgtgctc tccacgtcgg agatcgcggc
     3781 gctcgcgcag tagctacgcg ggctcgatct tcgacgccag gatctcgtcg atgatcccgc
     3841 cccagtcgcc ggttgcgccg agctgcgcga gaactgcgtt gagtccccat tgcagccggt
     3901 tgaggaacaa ccactcgggt ggcatcgcga gccggaaccg gttcggattg tcgaacatca
     3961 gcacgccgaa gctcttcgcg acgaacgccg ggttgtggcg gaatccgcgc tcgaggaacg
     4021 gcgtgtacag aaagcgcatc gcgttccact ggtagtccca gtcgaaccct ttctccttgc
     4081 cgacgaagcc gagcacgcga aaccgggtcg cgaagccttc gcgatcgcca acgatgacgg
     4141 cgcgcgccat ctctcgccag atctcgatca tggcgggctc gaagcggcgc acacagccga
     4201 agtcgaggaa cgtcacggtg ccgtccgctg cgaccagata gtttccggga tgcggatcgg
     4261 cgttgtagat gcagcgccgc cacatcagct cgaagcacgc gcggaagatc acctgacccg
     4321 cgcggtcgcg cgctgccgga tcggactgcg cgagcggcgc accgtcgccg agcacggttg
     4381 tcagcacgcg gcgactcgag cgctcgccga tcacagcggg cacgcccgca ccgtcgaccg
     4441 aggcgagcag ccgcgcgaac agcgtctgcg atgtagcctc gcgctcgtaa tcacactcct
     4501 cgagcaagcg gtcgcgcagc tcgccggcga gcccggcgcc atcgagcggc gatccgatcg
     4561 acgacaaccg cgcgatcacc gccaccatct tgaggtcgct gcgaatcgcg tcctcgatgc
     4621 cgggatactg aaccttcacc gcgaccgctt gcccgcggtg ggtcgcgcgg tgcacctgac
     4681 cgatcgaagc cgccgcgaac ggacgctcct cgatcgtttc gaatagctcg ctcgccggtg
     4741 caccgagctc ggtccgcagg acgtccgcga tccggtgaaa cgccatcggc gtcgattgcg
     4801 cctggagctc ggctagcacc tcctgcgcgg cgggcgggag cgagcccggc atgtagctcg
     4861 cgatctggcc ggccttcatg acgaggcctt tgaggttgcc catccgcgtc actagcttgc
     4921 gcgcggcggc gatcgcagcg tcggtgctgg cgagcgacgc gtcgatgtcg acatcgtctg
     4981 ccttgtcctt gcggcggaag aacatgcgcg aggccgcttt cgcgccgagc ttgctgctca
     5041 ggctcgcggt cgaccacatc cggcgtaggc caccggtcag ctcgtcgaga tcgtcgggca
     5101 ccgatgtgag atgccggacc acgcgtaacc gttgcagggg cgcccggtta tgatgcgcgg
     5161 tggatgaagg gcgcgacggt tctaacggtg tcgagccgtg cgctggtcga tgcgtgcgac
     5221 gcgctgggga tggacacggc ggcgctgctc gcggcgacgg ggctcacgcg cgccgaggtc
     5281 aataatcccg atgggcggat tccggtcgag aagatgaccg cgctctggcg cgaggcacat
     5341 gcgcgcgcag gcgaccccga tctcgcgctc cacgccgccg aggcgttgcc gttcggcgcg
     5401 tacgccgtga tcgatttctt ggctcgcacg agcgccacca tcggtgcggc gctcgaacgg
     5461 atctcgcgtt acttcccact gatcaacagc gtggtcgagc tgccgatcga gcgtggcagc
     5521 gactacgtcc gcctcgacat ggtcgatcga cgtgggccgg gcaagctgcc gcgcgcgtac
     5581 gccgagtacg tgctcgccgc tgtcgtgttg cgaacgcgag gggctgttgg cgtcgcgttt
     5641 ccactcgttc gcgtcgagct cgcatacgag gcgccggcct tggtttgcga gcacgagcgg
     5701 atcttcggct gtcccgtcgt attcggcacc gaacgcaccg gcatcgtgct cgcgcactca
     5761 gtggcggaca agtcgctcga gcgcggcgac tccggccttg ctgcagtgct cgagcgtcac
     5821 gcgcagctct tgatctcgca gctgccgccc gtgtccgatt cgatcagcga agttcgcgcg
     5881 acgatcaaag gtgagctgga gggaggcgat ccgagcctcg accatgtcgc gcgcaaactg
     5941 gggacgagtc gccgctcgct gcagcgccgg ctcgccgacg agaacctgac ttacgcgcaa
     6001 gtgctcgacg aagtccgctc gacgatggcg cgcgcgtatc tcggccggcc cgagctctcg
     6061 atcgccgagg tcgcttatct gctcgggttc tcggagcaga gctcgttcac gcgcgcgttt
     6121 cgccgctgga cgggcacgtc accggcggag tttcgccgca cgagcacgag ctgagcgacg
     6181 acgagattcg gtacccagca cgaccacgcg accgtcgcgt acgcgatcgc gccttgaacg
     6241 ccaagcaggc cgagtagggg gagctgtagc cgcaagctga cggccgcaaa tgcgagcgcg
     6301 tagctgcgcg ttatccatgc gcgatgaccg tcgcgatctc cggacagaat gcggtgaagg
     6361 ccaaccgccg tcgtcgtgag ccacgcgatc gcgagcgcgg tgaacccgac ctgcgcgacg
     6421 agaccgcccc acgcgttcgg ccccatcacg aagcccgcac tgccgccgag caggaccgcg
     6481 ctcacataga tgtagccagt cgcgcgatgc aaacgcggac ggcgcgcacg cagtctcggc
     6541 atgagctgcc acggtccggc gagcaatgcg atgccgccgc ccaccacgtg caccacaaac
     6601 acgagctgcg cgaccgtgta tcgcgcgagc aagtgctcga cgagcggcgg ctgattcgcg
     6661 gcgggctcga cgaggccgag cagccacggc aacggtgcga ggagcccaac gccgatcgaa
     6721 aggaacacca ccaacccgag ccgtagtcgc cgcatgtgct caaggtagtg acggctccgt
     6781 cgcgcgtctt tgaccgacgg cgtcagaccc ttacccgagc gcgccaatcg tcagatcgac
     6841 tgcgtcttct taccggctgt gacccagccc ttgatgccgt cgggcatgat gtaaacgttc
     6901 ttgtaaccga agctaatggc gcgcccggcg ccatcgtgac aagcgtgtca accagagtcc
     6961 gtgcagtaga agacgagctt cgtcgccttg tcgggtggca gctcactcgc ggggaagtcc
     7021 tcttcggagg tcaggatcgc gcccggcaat acgccgtact tcttgcgcgt gcgatcgccg
     7081 ttgcagtcga ccgcgactgc ttgcttgtcg gcaagcgcct tctcgacgtc gtccatcgtc
     7141 atcgtcggaa ggttgtctgt cgcggtggcc gccgagccgg tcttgtccgc ttgcttgtcc
     7201 ttcgcgcaac cggggatcga tgcgagcgcg agtgccgcgc cgagcaagta cttccgcatg
     7261 ggcgtagcgt actcgaatcg ccacgcgcgg tccggtgaaa ttctttatcc gggtgctagg
     7321 ggtttttgcg cgagcgcgtg ctgcggtcgc actagcgtcg gcgggatcga cgtcgtcagg
     7381 gcacggaacg cgagcggcag ccgaaacaga tgcgtcggcg gcttggtcat cccgaacacg
     7441 tggatgagcg cgaggcggac cgccggctca cgcgcggagc gtatgaacag ccgatccata
     7501 taggcgtagg ccaaacgatg cgcgaatttg cgttcgcctt cggtgcccgg gtaccgcagt
     7561 agcgcgtgcg cgtgacgcgc ttgcggaacg ccggagcgaa actccggctg cactgggaac
     7621 tgatcgcgat cgacgatcac gacctcgctc gcatactcgg ccagtacacg cgcggcgagg
     7681 agcccagcca tgctcccgcc catcaccacg gctcgctcga acaacatccg ctcatcgtat
     7741 cagcggatgc gtcgcgctta ctcgacgccg ggcactttgc gttcgggacg agcgccgtta
     7801 acgccgcccg ggttatccat cgctggccga tgcagcgcag ggtttgccgg ctcgcgtggg
     7861 gccggcggtg gcggggtcga tggatcaggc ggcggtggat tcacctgcca cgccggtacg
     7921 gttcccggcg gtagcgttcc ggtggtttgc ggcgtcgtct tccacggcgg cgtctcgtca
     7981 gggacggccg gcgtcgtgcc cggagttgcg gttgctcgtg acggcgtcgt gggtaccgcg
     8041 gcgttgctag gtttcgcggg ctcggtcgag ctgcgcagtc cttgcagcgt gagcacgaca
     8101 agcaacgcaa ccgcaacaca caagccggcg agcagccaga cagcgcgctt cgatggttgc
     8161 ggcattgtcc tcagtctgcg ttatctacgc gccccgcgga agctcgagac ggaacatgct
     8221 gccgttgccc tgcctgacga cgcggaggtc gccaccatgg ttctttgcga tgcgacgcga
     8281 aatcgtcagg ccgagcccgg tgcctttgcc gacgggcttg gtcgtaaaga acggatcgaa
     8341 gatccgctcg agtagctgcg gtgggacgcc aggtccgcta tcgccgacct cgatcacagc
     8401 cagcgcgccc tcgcttcgca cgctgagctg aacccacccc tgcggtccgg cggcaaacgc
     8461 cgcgttgtcg agcaagttga tcacgatctg ctcgatcaat ggtccggcgc agtacagcgg
     8521 ttcgtcgagc tcgatctgtt cgcgaatttc gacgttcgcg agctgtgtgg tgagcacgcg
     8581 gcgcgctcgc gcgatcagcg ctcggacgtc ctcggggcgc cggaccacct gttcgctgcg
     8641 cgagaaatcc aggatcctgc tgcaacgctc gcggatctgg ttcgctgcat cgacagcgat
     8701 ttcggccagc atcgcttcgg cggtattcga acccttctgg agctcgggca ggttcgccat
     8761 catcggttgc agcgcgttga cgagcgcgtt ggccggattg cgaagctcgt gcgcgaggcc
     8821 ggccgacagc attcccaacg ccgcgagctt ctcgctctcg tggagcttga gcgccatctt
     8881 gcgaatcgcg agctgcgacc gaattcgtgc caagagctcc tcggccgaaa acggtttcag
     8941 gacgtagtcg acggcgccgg cctcgaatcc agccaggcgt tcgccgagcc ctgcgtgtgc
     9001 gctgacgatg agaacaggcg agagcgcggt gccctgcagc gcgagaaact cccgcgtgag
     9061 atcgaggccg ttcatctcgg gcatctcgag atcggtcacg agcagatcgg ggatgtgttc
     9121 cttggcgagc gcgagtgcgg ccttgccgtt cgtcgccgcg agcacgcggt accgaccgga
     9181 gagcagatcg gtgatgtgct gcgcgagtcc aagatcgtcc tcggcaagca cgatcgtcgc
     9241 ttccggttcg cgcggctcag ccgtctcgcg cgacgccgcg cgcacattac gtgcagtcgg
     9301 atcctcctcg agtgcgctcg gtcgctgcag cggttcttct tgcggcagaa tcgctgagcc
     9361 cgtctgaaag cgtggcagcg tgacctcgaa gctcgttccg ctcggatcgt catgacggtc
     9421 cacgcggatc gtgccgtgct ggagctcgac gagctccttc gacagtgcga gtccgatacc
     9481 cgtaccgcgt acgccacgtc ccacggccgt cgcggctcgc tcgaaccggc cgaacagctt
     9541 ggggatgaac tcgggctcga tgccggggcc agtgtcgcgc acgttgaccg tgaccgcgtg
     9601 ttccttcgcc gagagcgaga ccgtgatccg gcctccgggc ggcgagaact tcaccgcgtt
     9661 cgagatgaag tttccgacga tcgtctccag cgcagcttcg tctatcgtgg cggcgcattc
     9721 gtcgggaccg acgaacgtga tctcgaggtc acctttctgc gcggccgtca cccagttccg
     9781 cgtcaaccgc cgcaagcagg tcgccacatc gcagggaccg ggacgaacgc gtagcttgcc
     9841 ctcgtcgccg gcggcaagca gcagcaggct gttgaccagg tgcaacaacc gccgtgtgct
     9901 cgcgttgatc tcgtcgagat tgcgccgcgt gtttccgctg agcgcttcgc ccgaacgcat
     9961 gatcgcatcc gacgcgagct ggatcagcgt gagcggcgtg cggatctcgt ggttgatgtt
    10021 cgcgaagaat cggtctcgcg ccgccttcgc ttcgacgagc tgctcgttgg cctcggcgag
    10081 ctgtgcagta cgttcgacga ccttgtcttc gagggtccgc ttgaactgga gcacctcggt
    10141 gaacgagatc gcgttgtcga cgctcatcca gatcgtcggc agcacgtagt cgacgagctc
    10201 gatcagttct tcttcgttct gttctggcgt gtgatggccg gtgagcgtcc cgacttcgac
    10261 gccgcgcgcc atgacctgcc actccatggc cttaccctcg agtacccccg cttgcgcggc
    10321 gctcgtctcg tgaagctcct cgaa
//
LOCUS       DASUFX010000150         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_23529,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000150 DASUFX010000000
VERSION     DASUFX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23529"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFV99_05940"
     CDS             <1..298
                     /locus_tag="VFV99_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058880.1"
                     /translation="ARTLATFSNAVAQVWAHVLRAGSAIRAGDKSRGEAATAAVEQGA
                     LAAGMKLTSAAARFRLAELRGDDSLLATASAEMTKLGIRVPRKMTALLFPIGER"
     gene            461..1192
                     /locus_tag="VFV99_05945"
     CDS             461..1192
                     /locus_tag="VFV99_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058881.1"
                     /translation="MIRNGVMVVAAATLMGGCFSPGSSEENTSQMAFGLGGGDFAGNS
                     VRICGQRQMAADSKYFCDSVLPGDSSSVEVPGPHPMEECPCFDFASDGTLVDPTTHAP
                     VVINGLCPSIDLPHAPWRFDYTIYTQTSCHGSVLNTAANNFTCFDSKDLASQDHPNSS
                     IERLYPGANDNHVLCTTENSSKTWEFESCAITTTSADEQACRTRYDCDCAPVNSHCAC
                     GGLVASDLEQGCHFEAGTCDILCNN"
     gene            1360..1719
                     /locus_tag="VFV99_05950"
     CDS             1360..1719
                     /locus_tag="VFV99_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058882.1"
                     /translation="MAPRRLLGCLPAAALAVCMWTGCKKDAAPTGLDKRCEQLAKACG
                     DKDKHIEKITGECKQAAQKQTEKTCMDKAIAAYDCYEKELCGGEKVWALDDFRVLADR
                     HKKCTAERDALKECTGK"
     gene            1855..2754
                     /locus_tag="VFV99_05955"
     CDS             1855..2754
                     /locus_tag="VFV99_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058883.1"
                     /translation="MVINHRFQITLAGVACALVAWVAPAAADSFDCRKNETQAECHAR
                     LKCKANEELEDCQKRLRAAAQSQGNGNNQDDERSGRDRQSDERSGRDNSDRDRDDRGG
                     DDNGRRDRGDRGGDDNGRRERGGGGGGDRGSRRRGGGGGGGFEANKTFGIGLELGEPT
                     GINGKYFFGPKIALDFGLGYIYRHYYYRRGEGLHVYGDVLFHPVSLVHADAFELPLYI
                     GGGLRFWDFDYCIGNDCAHSGSAIGIRVPIGISFDFNNVPLDFFLQVVPVIDFIRGDY
                     YDQYGDREHFGADFSLGIRFWFK"
     gene            2913..3611
                     /locus_tag="VFV99_05960"
     CDS             2913..3611
                     /locus_tag="VFV99_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX5058884.1"
                     /translation="MRSSMLRALLLASALLAPACVADDDPASPDGLDPSSGTGSDRGS
                     DSGSGSNETPPGPVEKVLDHDYQVQQTGYWCGPAATRVVLSARISPPSQQTLANELGT
                     TVNGTDWIGQVTAVLNDDLGGNYYKTTEMPNDPPTQPQRDRLWNDVVHSIDNDFAIVA
                     NIVAPPGNQPPGYPSNQTIYHYFAVVGYNSEKREVYIADSANFSGYQHYWLTLDKLAT
                     LIPPKGYSALDPAQ"
BASE COUNT          706 a         1190 c         1147 g          610 t
ORIGIN      
        1 cgcccgcacc ctcgccacgt tctcgaatgc ggtcgcccag gtctgggcgc acgtgcttcg
       61 cgccggctcc gcgatccgcg ccggcgacaa atcgcgcggt gaagcagcga cagccgccgt
      121 cgagcaaggt gccctggccg ccggcatgaa gctcacgtcc gccgcggcgc gcttccggct
      181 tgccgagctg cgcggcgacg attcacttct cgccaccgcc tccgccgaga tgacgaagct
      241 gggcattcgt gtaccgcgaa aaatgaccgc actgctgttc ccgatcggcg agcgctgaca
      301 cgcgaagggg acctcatgcg cgtgccgctg cgcgcaacgt gacttcaatg cacatcgatc
      361 gcgttcacgt gatcgccgta tcgcaattcg tgcgcccacg cgcggtacgt ccgttgcaaa
      421 gggggccgcc gcgaatcagc gcaagaagag agaggaagca atgatcagga acggagtgat
      481 ggtggtggcg gcggcgacgt tgatgggcgg atgtttttct ccgggcagct cggaagagaa
      541 cacgtcgcag atggccttcg gactcggcgg cggcgacttt gccggcaact ccgtccggat
      601 ctgcggccag cgtcagatgg cagccgacag caagtacttc tgcgattccg tgttgccagg
      661 cgatagcagc tcggtcgagg ttccggggcc gcacccgatg gaagagtgcc cgtgcttcga
      721 cttcgcctca gacgggacgc tcgtcgatcc gacgacacat gcccccgtcg tgatcaatgg
      781 cctgtgtccg tccatcgacc tgccgcacgc gccgtggcgg ttcgactaca cgatctatac
      841 gcagacgtcg tgtcatggct cggtgctcaa cacggcggcg aacaacttca cgtgcttcga
      901 ctcgaaggac ctcgcgtcgc aggatcatcc caactcgtcg atcgagcgtc tgtacccggg
      961 tgccaacgac aatcacgttc tctgcaccac ggagaactcg tcgaagacgt gggagttcga
     1021 gagctgcgcg atcacgacga cctcggcgga cgagcaagcg tgccggacgc gctacgattg
     1081 cgattgcgcg ccggtgaaca gccactgcgc gtgtggcggt ctcgtcgcct ccgacctcga
     1141 gcagggctgc cacttcgaag caggcacgtg cgacatcctc tgcaacaact gagggctccg
     1201 cgttagcatc ggcagcgatg cccgtttgtg tccgagcagt atgcggcgct cgagcgcgat
     1261 ggcgtcgcag cgctacatgt gtatccagtc acgggcacga atcagctcgg cgtcgacgag
     1321 catctgcctg cgtaacagcg attgaggtat gctcgcggca tggcacctcg acgacttctc
     1381 ggatgccttc ccgccgcggc gctggccgtg tgcatgtgga caggctgcaa gaaagatgcg
     1441 gcacccaccg gcctcgacaa acgctgcgag cagctggcca aggcgtgcgg cgacaaagac
     1501 aagcacatcg aaaagatcac aggcgagtgc aagcaggctg cgcagaagca gaccgagaag
     1561 acttgcatgg ataaagcaat cgccgcgtac gactgctacg agaaagaact atgtggcggc
     1621 gagaaggtgt gggcgctcga cgacttccgc gtcctcgccg atcgccacaa gaagtgcacc
     1681 gcagaacgcg atgcgctcaa agagtgcacc ggcaaatagc cgccacgcac cgagcacgag
     1741 ctagccccgt acgcaagctt gcaaacgcgc cgcgcgtcgc gatcgaccgc gctcgctttc
     1801 gcgtgtatcc gaatgatgcg acgatgcacg aaccaacgtc ggggaggact tcgcttggtt
     1861 atcaaccatc gattccagat cacattggcc ggtgtcgcgt gtgcgctcgt cgcctgggta
     1921 gctcctgctg cggcggacag tttcgactgc cgcaagaacg agacgcaggc cgaatgtcac
     1981 gcgcgtctga agtgcaaggc gaacgaagag ctcgaggact gtcagaagcg actgcgagcc
     2041 gctgcccagt cacaaggcaa cggtaacaac caggacgacg agcgcagcgg acgcgatcgt
     2101 cagagcgacg agcgtagcgg acgcgacaac tcggaccgcg atcgcgacga ccgtggtggc
     2161 gatgacaacg gccggcgtga ccgcggcgat cgcggcggtg atgacaacgg ccggcgcgag
     2221 cgcggcggcg gtggtggcgg cgatcgcgga agccgtcgtc gtggcggtgg cggcggtggc
     2281 ggcttcgagg caaacaagac gttcggtatc ggcctcgagc tcggcgagcc aaccggcatc
     2341 aacggtaagt acttcttcgg gcccaagatc gcgctcgact ttggtctcgg ctacatctac
     2401 cggcattact actaccgccg cggcgaggga cttcacgtct acggcgacgt cttgttccac
     2461 ccggtgtcgc tcgtccatgc cgacgcgttc gagttgccgc tctacattgg cggcggtctg
     2521 cggttctggg acttcgacta ctgcatcggc aatgactgcg cccacagcgg ctccgcgatc
     2581 ggcatccgcg ttccgatcgg catctcgttc gacttcaata acgtgccgct cgatttcttc
     2641 ctgcaggtcg tcccggtgat cgacttcatt cgcggcgact actacgacca atatggtgac
     2701 cgagagcact tcggtgccga cttctcgctc ggcattcgct tctggttcaa gtaggcggcg
     2761 tgtaggctga cagctcctga agagcacagc tcagcgccac cagtcggttg cactggctgt
     2821 ttgcactgag gacagccgtc cctctgcgag ccgcgcaaac gccgaattgc gcgagcgaaa
     2881 tgttggtacg catgatgtag ctagcagacg gcatgcgctc ctcgatgctc cgcgcgctgc
     2941 ttctcgccag cgccctgctc gcccctgcct gcgtcgccga cgacgatccc gcctcgcccg
     3001 atggactcga tccgtcgagc ggtactggct cggatcgcgg ctccgacagc ggatctggat
     3061 ccaacgagac gccacccggc cccgtcgaga aggttctcga tcacgactac caggtgcagc
     3121 agaccggcta ctggtgcgga cccgctgcca cacgcgtcgt gctgtcggcg cgcatctcgc
     3181 caccgagcca gcagacgctc gcgaacgagc tcggcacgac cgtgaacggc accgactgga
     3241 tcggtcaggt cacggcggtg ctcaacgacg acctcggcgg caattactac aagaccaccg
     3301 agatgccgaa cgatccgccg acgcaaccgc aacgcgaccg gctgtggaac gacgtcgtgc
     3361 acagcatcga caacgacttc gcgatcgtcg cgaacatcgt cgcaccgccc ggcaatcagc
     3421 cgcccggcta tccgagcaac cagaccatct atcactactt cgcggtcgtc ggttacaaca
     3481 gcgagaagcg cgaggtctac atcgccgact cggcgaactt cagcggctac cagcactact
     3541 ggctgacgct cgacaagctc gcgacactga tcccgccgaa gggttactcg gcgctcgacc
     3601 ccgcgcagtg agctagtgcg cggttagcgc gtgaagttca gcgacagcac gcc
//
LOCUS       DASUFX010000151         8680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1273882, whole genome shotgun sequence.
ACCESSION   DASUFX010000151 DASUFX010000000
VERSION     DASUFX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273882"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFV99_05965"
     CDS             complement(<1..1126)
                     /locus_tag="VFV99_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HEX5058885.1"
                     /translation="MRGTLLLLCALTACTVEASRSDHAITNGTVDTGDPAVVALVDTS
                     DIVACTASVIGAHTAITAAHCFINRAPHSLRVAFGSSLADATFAQIADARSHPDFDPA
                     TLAHDVAMVTFRDESPVAPLVLDTRTIDASLIGTMFRAVGFGVTASTAADAGGKREGM
                     AQISEVTAEEFTAMPSPSQPCRGDSGGPALLASDAVAAVVSRGDGACSDHAIYARIDV
                     ARAPLVDPYLADTAPSTAHTGDACFYDGHCSEGSCLQTHDDPLLYFCSQPCARDADCP
                     AAMECASDGCRYPEPSPGALGSACDQDPQCTSDTCRESVCTISCVLDTSACPAGYECR
                     GVGNGEYCFAAADDGCGCASSGTSAPLWLVVIWLARARRRS"
     gene            1260..1862
                     /locus_tag="VFV99_05970"
     CDS             1260..1862
                     /locus_tag="VFV99_05970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5058886.1"
                     /translation="MDKLPGVLRRGPLTSKRVALTFDDGPDEMTLQYLEMLDDLGVPA
                     TFFVCGKLAAEHPELIREYLRRGHQVANHGYDHTRFTKLSRRALLDQLDRTDRVIGGQ
                     VSGRSWVRPPHGTLDATSLLAMRTAGYVVALWSIDSCDYDVRDPALLAEKCSPAIVGA
                     GDVLLFHEGQPWTLEALPRIVAALHGSGLECVTMHDLFAR"
     gene            complement(1859..2785)
                     /locus_tag="VFV99_05975"
     CDS             complement(1859..2785)
                     /locus_tag="VFV99_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5058887.1"
                     /translation="MDWRVKGAIQKALGYVPGGDRLHYMLQRYAGGMRDFGRECDIKV
                     DDWRLMMGHLRAAQIPVEGATLVEIGTGWYPTFPFCLYLAGVAKVFTFDLHRLIKSDM
                     VYLLADRLAAHVALVAREARRSEDEVKAEQVALVTAMTNGASLTFATGGVIDYRAPAD
                     ASETSLLSETVDVVFSNSVLEHVPRPVIEACFTEAMRILRPGGVMFHSVNCGDHYAYS
                     DRTIDQLHYLQYSEADWAKWNNEFLYQNRLRAIEFIDMARHAGFTIEIDTSRASPQRL
                     AQLDQITVDPVFAHYTRDQLAITSVDFIARKP"
     gene            complement(2796..4004)
                     /locus_tag="VFV99_05980"
     CDS             complement(2796..4004)
                     /locus_tag="VFV99_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5058888.1"
                     /translation="MIGAGLSGLVCATRLVAGGATVRVLEARERVGGRLHTGTVGGAS
                     VDLGGQWMTAGQPKLAALAGELGVAIEHHDRAGKALVDDRGGLLAAFGQWRVMRRIEK
                     LMKQLDRELDNQSLGQWFAREIRHSVARERIALHADLIFATDPSSLSLLHYLTTMNAT
                     SGFRPESTDEYRFSGGAQGLALRLAEGLSDRVQLGEPVTAIEQDEDGVTVRARESYRA
                     KRVVLAIPPPLVKKLAVDLPPGARAYVNAAFSGRVVKCFAAYAKPAWRDRGYSGESYH
                     PRGAVRATVEIAGEPPVLLAFVVGTEAARWSTREPEDRKREALAAFAPFSADPPLDYL
                     EVDWGSDPWSAGCVPALPPRALSAGARWRECHGRLHIAGTESAAQWPGYMEGAIDAGE
                     RAATEVLAAL"
     gene            4156..4776
                     /locus_tag="VFV99_05985"
     CDS             4156..4776
                     /locus_tag="VFV99_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5058889.1"
                     /translation="MPRLKQETAKSIQENVEAIALDLFVRRGFAATTTREIATAAGYT
                     AGALYAHYPSKEALFAAVVARYRRKLADDNVLADALRRTSFPHDIRELARAIKSLIKE
                     NRAYWMLWYIDVLEFDSKHFKSQLAPKALIAQPEVRTRLLQLRGKRVLRIEPELAFTM
                     VYMHLFNYFLIETVFGGDEHYGISEAKAVDTIADVFLHGVLAKEAA"
     gene            4773..5972
                     /locus_tag="VFV99_05990"
     CDS             4773..5972
                     /locus_tag="VFV99_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5058890.1"
                     /translation="MTVVDFLDRSEEAMAAQLSDALANDTVLHMGAQLWRIGLAGIAA
                     LPPALRNLAPPPLAREALFEVLRAGWSVAARSFALYPLAQHIERTPLPAFEPTPARVV
                     HVTSDYQLHAIEGTGEPILIVSSMINHWYVLDLDDNHSFLAMLRTLGRPIYVLDWLAA
                     KPADDRSFGEICAGPIRSAVDHICAAHDVDALSIMGYSMGGTLATCFAARFPERIARL
                     ATLCAPIRFDNAGAFSRWLSADLVDVNLVAAAWDRVPSQLVHMPFWYLHPTVKLRKLV
                     QLARSFEKPGYLDHFLATETWNHDNVDVPRGVFRSWIGELYQRNALLAGEVVIGGRAA
                     SLDAIRCPKLVISGASDTITPPASAEALPGARILRLDAGHVGVLTSRRALAAQAAALS
                     TWLGERA"
     gene            5969..7405
                     /locus_tag="VFV99_05995"
     CDS             5969..7405
                     /locus_tag="VFV99_05995"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5058891.1"
                     /translation="MKTVDQYLDSLKARNVRLFVRGQLVKDPVEHPLVAPSVRTVAET
                     YRLAHEPDTRELFTAHSRFIDGPVNRFTHIFEAPEDLTNKVLMQRTLGRYTGTCFQRC
                     VGMDALNALYTTTHAMDARDGTQFHARFVEFLQHVQREDLVLCGAMTDPKGDRRKAPI
                     EQPDMYLRIVARTPNSIVVRGAKMHQTGVVNSHMIVAMPGRALTPDEADFAVAFAIPV
                     DAKGITLVYGRQPSDDRRAGCDIDQGNARYGGQEAIVLFEDVEVPLEHVFMAGQTGWA
                     APLVDMFAGYHRQSYGGCKPGNGDVLIGAAALLAKLNGVEKVPHVRDKLVEMTHLNET
                     MHAGGLACSSKCTRTPAGTYMVDPLLANVTKQNVTRMPYEMARLAEDIAGGLLVTLPA
                     AEDFDHPEVGPLLRKYVQGATGSDEDRRRVLRLVETMTIGATAVAFRTESMHGAGSPQ
                     AQRMRIERQGDFEAKMGDAKRIAGLRRD"
     gene            complement(7547..7834)
                     /locus_tag="VFV99_06000"
     CDS             complement(7547..7834)
                     /locus_tag="VFV99_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058892.1"
                     /translation="MRFLSVIVACLMFVGCKDASKDFEQLADRACECGQEDLACGNKV
                     LAEVSKFTETHKMSDGDIKRFNEAGKRINECLGSAGILPNQLTAALEKMGR"
BASE COUNT         1473 a         3023 c         2699 g         1485 t
ORIGIN      
        1 gcgatcgccg ccttgcgcgc gcgagccaga tgacgacaag ccacagtggc gcactggttc
       61 cactgctcgc gcagccgcag ccatcgtcgg ccgcagcgaa gcaatactct ccgttgccaa
      121 cgccacggca ctcgtagccg gcaggacacg cgctcgtgtc gagcacgcag ctgatcgtgc
      181 acacgctctc gcgacacgtg tcactcgtgc actgcggatc ctggtcgcac gccgagccga
      241 gcgcgccagg tgatggctcc ggataccgac aaccatcgct cgcgcactcc atcgccgcgg
      301 ggcaatcggc gtcgcgcgcg cacggttgcg agcaaaagta gagcaacgga tcgtcgtgcg
      361 tctgcaagca cgagccctcg ctgcaatgtc cgtcgtagaa gcacgcatca ccggtgtggg
      421 ctgtactcgg cgcggtatcc gcgaggtacg gatcgacgag cggtgcgcgc gcgacgtcga
      481 tgcgcgcata gatcgcgtga tcggagcatg cgccgtcgcc gcgactcacg accgccgcga
      541 ccgcgtcgct cgccagcagc gccggtccgc ccgaatcacc gcggcacggc tgcgacgggc
      601 ttggcatcgc ggtgaattcc tccgccgtca cttccgagat ctgcgccatg ccttcgcgct
      661 tcccgccggc atcggccgcc gtgctcgcgg tcacgccaaa tccgaccgcg cgaaacatgg
      721 tgccgatcag cgacgcatcg atcgtgcgcg tgtcgaggac cagcggcgcg accggcgatt
      781 cgtcgcgaaa cgtcaccatc gcaacgtcgt gagcgagggt cgccggatcg aaatcgggat
      841 gtgagcgcgc atcggcaatt tgcgcgaacg tcgcgtcggc gagcgagctt ccgaacgcga
      901 cacgcaacga atgcggggct cggttgatga agcaatgcgc cgccgtgatc gcagtgtgcg
      961 ctccgatcac gctcgcggtg cacgcgacga tgtcactggt gtcgacgagc gcgaccaccg
     1021 ccggatcgcc ggtgtcgacc gtgccattcg tgatcgcgtg gtcgctgcgg ctcgcttcga
     1081 ccgtgcacgc ggtgagcgcg cacagaagca acagcgttcc gcgcatgtca catcgtaaca
     1141 tcaatgagtt atcggtctgg ctgcggtctc acctacgcgg ccttgttggc ctgccgaaaa
     1201 ctcggtacgc agtgatcaac gtggctactt ccgtacgctc gagggtcaag aacgccgtca
     1261 tggacaagct tcccggcgtg ctgcgccgcg gtccactgac gtcgaagcgc gtcgcgctca
     1321 cgttcgacga cggtcccgac gagatgacgc tccagtacct cgagatgctc gatgatctcg
     1381 gcgtacccgc aacgttcttc gtgtgcggca agctcgccgc cgaacacccc gagctgattc
     1441 gcgagtacct gcgacgcggt catcaggttg cgaaccacgg ctacgaccac acgcgcttca
     1501 cgaagctgtc gcgccgggcg ctcctcgacc agctcgatcg caccgaccgc gtgatcggcg
     1561 gtcaggtcag cggccgctcg tgggttcgtc ctccacatgg cacgctcgac gcgacctcgc
     1621 tgctcgcgat gcgcaccgcc ggctatgtcg tcgcattgtg gtcgatcgat tcgtgcgact
     1681 acgacgtgcg cgaccctgcg ctactcgccg agaagtgcag ccctgcgatt gtcggtgccg
     1741 gcgatgtcct gctgttccac gagggccagc cgtggacgct cgaagcactg ccgcggatcg
     1801 tcgccgcgct ccacggcagt ggcctcgagt gcgtgacgat gcacgacctg ttcgcccgct
     1861 agggcttgcg cgcgatgaag tcgacgctcg tgatcgcgag ctggtcacgc gtgtagtgcg
     1921 caaacaccgg atcgaccgtg atttgatcga gctgcgcgag ccgctggggt gacgctcgcg
     1981 atgtatcgat ctcgatcgtg aagccggcgt gtctcgccat gtcgatgaac tcgatcgcgc
     2041 gcagccggtt ctggtagagg aactcgttgt tccacttcgc ccagtccgcc tcggagtact
     2101 gcagatagtg gagctgatcg atcgtgcgat cgctgtacgc gtagtgatcg ccgcagttga
     2161 ccgagtggaa catcacaccg cccggacgca gaattcgcat ggcctcggtg aagcacgctt
     2221 cgatcaccgg tcgcggcacg tgctcgagca cgctgttcga gaacacgacg tcgaccgtct
     2281 cggaaagcaa tgacgtctcg gacgcgtcgg ccggagcgcg atagtcgatc accccgccgg
     2341 tcgcgaacgt gagtgacgcg ccgttggtca tcgcggtgac gagcgcgact tgctcggcct
     2401 tcacttcgtc ttcggagcga cgtgcctcgc gcgcaaccag tgcgacatga gccgcgagtc
     2461 gatcagcaag caggtagacc atgtctgatt tgatcagccg gtgcagatcg aacgtgaaga
     2521 ccttcgcgac gccggcgaga tacagacaga acggaaacgt cgggtaccag ccggttccga
     2581 tctcgacgag cgtcgcgccc tcgacgggaa tctgcgccgc acgcagatgt cccatcatca
     2641 gtcgccagtc gtcgaccttg atatcgcatt cgcgaccgaa gtcgcgcatg ccgcccgcat
     2701 agcgctgcag catgtaatga aggcggtcgc cgccgggaac gtagccgagc gctttttgga
     2761 tcgcgccttt gactcgccag tccatctggc gcgaatcata gcgccgcgag cacctcggtc
     2821 gcggctcgct cacctgcgtc gatcgctcct tccatgtagc ccggccactg cgcagcgctc
     2881 tcggtgcccg cgatgtgaag gcgaccgtga cactcgcgcc agcgtgcgcc agcgctcagt
     2941 gcccgtggcg gcaatgctgg aacacaaccc gcactccatg gatcgctacc ccagtcgact
     3001 tcgagatagt cgagcggagg gtctgcgcta aacggcgcaa acgccgcgag cgcttcccgc
     3061 ttgcgatcct ctggctcgcg cgtcgaccat cgcgctgcct cggtgccgac gacgaacgcg
     3121 agtaacaccg gcggctcgcc ggcgatctcg acggtcgcgc gcaccgcgcc gcgcgggtga
     3181 tagctctcgc cggagtagcc gcgatcacgc cacgccggct ttgcgtaggc agcgaagcat
     3241 ttgacgaccc gaccagagaa cgccgcattg acgtacgccc gcgcccccgg cggcaaatcg
     3301 accgcgagct tcttcacgag cggcggcgga atcgcgagca cgacgcgctt ggcccgatac
     3361 gactcccgcg cgcgtaccgt cacgccgtcc tcatcctgct cgatcgcggt cacgggttcg
     3421 ccgagctgca cgcggtcact cagcccctcc gcgagccgca gcgcaagccc ctgcgcgccg
     3481 ccgctgaacc ggtactcgtc cgtcgactcc ggccgaaacc cgctagtcgc gttcatcgta
     3541 gtcaggtagt gcagcagcga caggctcgac ggatcggtcg caaagatcag atccgcatgc
     3601 agcgcgatcc gctcccgcgc gacagagtgc cggatctcgc gcgcgaacca ttggccgagc
     3661 gattgattgt cgagctcgcg atcgagctgc ttcataagct tctcgatgcg ccgcatcacg
     3721 cgccactgac cgaacgccgc gagcaggccg ccgcgatcat cgacgagcgc cttgccagca
     3781 cgatcatggt gttcgatcgc gacgccgagc tcacccgcaa gtgcggcgag cttcggctgc
     3841 cccgcggtca tccactgccc gccgagatcg accgaggcgc cgccgacggt gccggtgtgc
     3901 aaccggcccc cgactcgctc ccgcgcctcg agcacgcgca cggtggcgcc gcccgcgacg
     3961 agtcgagtcg cgcacaccag gcccgagaga ccggctccga tcacgagtac gtctgtcacg
     4021 gtagaggagc tggctgccga ggcacggtag ggggctggct ggcgaggcag cgaggctccg
     4081 ccaccgattc tagtcgcccg ccgaatccag gttgacctgg gccccgaatc tctgctaaat
     4141 gagcgctcgt ttaatatgcc tcggctcaag caagagaccg cgaagtcgat ccaggagaac
     4201 gtcgaagcga tcgcgctcga cctattcgta cgccgcggct ttgcggcaac caccacccgc
     4261 gagatcgcca cggccgccgg ctacaccgct ggcgcccttt acgcgcacta cccgagcaag
     4321 gaggcgctgt ttgccgcggt cgtggcgcgc tatcgccgca agctcgccga tgacaacgta
     4381 cttgccgatg cgttgcgccg cacgagcttc ccgcacgaca tccgcgagct cgcgcgcgcg
     4441 atcaagtcgc tcatcaagga gaaccgtgcg tactggatgc tctggtacat cgacgtgctc
     4501 gagttcgaca gcaagcactt caagtcgcag ctcgcaccga aggcactgat cgcgcagccc
     4561 gaggtgcgca cgcgtctgct gcaactgcgt ggcaagcgcg tactgcgcat cgagcccgag
     4621 ctcgcgttca cgatggtgta catgcacctg ttcaactact tcttgatcga gaccgtgttc
     4681 ggcggcgacg agcactatgg catctcggag gcgaaagccg tcgacacgat cgccgacgtc
     4741 tttctgcatg gcgtgctcgc caaggaggcc gcgtgaccgt cgtcgacttt ctcgatcgca
     4801 gcgaagaggc gatggccgcg caactgtcgg acgcgctcgc gaacgacacc gtgctgcaca
     4861 tgggcgcgca gctctggcgc atcggacttg ccggcatcgc cgcgttgcca cccgcactac
     4921 gcaatctcgc gcctcctccc ctcgcgcgcg aggcgctctt cgaggtgctg cgcgccgggt
     4981 ggagcgttgc ggcgcgcagc ttcgcgctgt atccgctcgc gcagcacatc gagcgtacgc
     5041 cgttgcccgc attcgagccg acacccgcac gcgtcgtgca cgtgacgagc gattaccagc
     5101 tccacgcgat cgaaggcacc ggcgagccaa tcctgatcgt gtcatcgatg atcaaccatt
     5161 ggtacgtcct cgatctcgac gacaaccact cgttcctcgc gatgctgcgc acgctcggcc
     5221 gcccgatcta cgtgctcgac tggctcgccg cgaaacccgc cgacgaccgc agcttcggcg
     5281 agatctgcgc cggaccgatt cgctccgcgg tcgatcacat ctgcgccgcg cacgacgtcg
     5341 atgcgctctc gatcatgggt tactcgatgg gcggtacgct cgcgacgtgt ttcgcggcgc
     5401 gctttcccga gcgcatcgcg cggctcgcga cgctctgcgc accgatccgc ttcgacaacg
     5461 cgggcgcgtt ctcgcgctgg ctctcggcgg atctcgtcga cgtcaatctg gtcgccgccg
     5521 cctgggaccg cgttccgtcg cagctcgtac acatgccgtt ctggtacctg cacccgaccg
     5581 tcaagctgcg caagctcgtg cagctcgcgc gcagcttcga gaagccgggc tatctcgatc
     5641 acttcctggc gaccgagacg tggaatcacg acaacgtcga cgttccgcgc ggtgtgtttc
     5701 gttcatggat cggcgagctc tatcagcgca atgcgctcct cgccggcgag gtcgtgatcg
     5761 gcggtcgcgc cgcttcactg gacgcgattc gctgtccgaa gctcgttatc tccggcgcct
     5821 ccgacacgat caccccaccc gcttccgccg aggctctccc cggcgctcgc atcttacggc
     5881 tcgacgctgg gcacgttggc gtgctcacct cgagacgtgc gctcgccgca caagccgcgg
     5941 ccctttcgac ctggctagga gaacgtgcat gaagaccgtc gaccaatatc tggacagcct
     6001 caaagctcgc aacgtgcgcc tgttcgtgcg cggccagctc gtcaaagatc cggtcgagca
     6061 tccgctcgtc gcaccgagcg tgcgcaccgt cgccgagacc taccggctcg cgcacgagcc
     6121 cgacacgcgc gagctgttca cggcgcactc gcggttcatc gacggcccgg tcaatcgctt
     6181 cacccatatc ttcgaagcac ccgaggacct gacgaacaag gtgctcatgc aacgcacgct
     6241 gggccgctac accggcacgt gcttccagcg ctgcgtcggc atggatgcgc tcaacgcgct
     6301 ctacacgacc acgcacgcga tggacgcgcg cgacggcacg cagttccacg cccgcttcgt
     6361 cgagttcctc cagcatgtcc agcgcgagga cctcgtgctg tgcggcgcga tgaccgatcc
     6421 gaaaggcgac cgccgcaagg cgccgatcga gcagcccgac atgtacctgc gcatcgtcgc
     6481 gcgcacaccg aattcgatcg tcgtgcgcgg cgccaagatg caccagaccg gcgtcgtcaa
     6541 ctcgcacatg atcgtcgcga tgccgggccg cgcgctaaca cctgacgagg ccgacttcgc
     6601 ggtcgcgttc gccattcccg tcgacgccaa gggcatcacg ctggtctacg gccgtcagcc
     6661 gagcgacgat cgccgcgccg gttgcgatat cgatcaaggc aacgcacgct acggcggcca
     6721 ggaggcgatc gtgctgttcg aggacgtcga ggtcccgctg gagcacgtct tcatggcggg
     6781 ccagaccggc tgggccgcgc cgctcgtcga catgtttgcg ggctatcacc gccagagcta
     6841 cggcggctgc aagccaggca acggcgacgt cttgatcggc gcagctgcat tactcgccaa
     6901 gctcaatggc gtcgagaagg tgccgcacgt ccgcgacaag ctcgtcgaga tgacgcacct
     6961 caacgagacc atgcacgccg gcggtctcgc gtgctcgagc aagtgcacac gcacaccggc
     7021 tggcacctac atggtcgatc cgctgctcgc gaacgtgacg aagcaaaacg tcacgcgcat
     7081 gccctacgag atggcgcgcc ttgccgagga catcgccggc ggcttgctcg tcacgctccc
     7141 cgccgccgag gacttcgatc atccggaggt cggtccgctg ctgcgcaagt acgtccaagg
     7201 tgcgaccggc agcgacgaag atcgtcgccg cgtcctgcgc ctcgtcgaga cgatgacgat
     7261 cggcgcgacc gcggtcgcgt tccgcaccga gtcgatgcac ggcgcgggct cgccgcaggc
     7321 gcagcgcatg cgcatcgagc ggcaaggcga cttcgaggcg aagatgggcg acgcgaagcg
     7381 catcgccggt ctgcgccgcg actagccctc gcacgctatg cacggccact cgcccctcgc
     7441 cggcctgcgc cgcgactagt ccctcgcacg ctacgcgcgg ccactcgccc ctcgccggcc
     7501 tgcgccgcga cagccccttc gaccgacggt ttgcccgacg gcggtgctag cggcccatct
     7561 tctcgagcgc cgcagtcagc tggttcggca ggatgccggc actcccgagg cactcgttga
     7621 tccgcttgcc cgcctcgttg aaccgcttga tgtcgccatc cgacatcttg tgggtctcgg
     7681 tgaacttcga gacttcggcc aggaccttat ttccgcacgc tagatcctct tgcccacatt
     7741 cgcaggcgcg gtcggcaagc tgttcgaagt ccttcgacgc gtccttgcaa ccaacaaaca
     7801 tcaggcaggc cacgatgacg gagagaaaac gcattgccac cgtcttatct cagcgctcag
     7861 gtacttcgcg gttagcgatc cggacgcact gggtgtctcg cgatcgctga tcgcgatcgc
     7921 tgatcgcgat cgacagatgt cgcgcttcgg cgattgcatt tccgagacgc tcgaccggga
     7981 ccttcaggat cccttcgatc cgctgtacgt ggtgtttgcg gtgacgcagg cccggcagtc
     8041 tcgcgtcaac ccatggtgcc gacgagctac ataggtaggg ttctgcgacg atctggttag
     8101 tacgccgtta tgaccgcggt gtgatcagag agccggacca tgtgcagggt ctcctgccac
     8161 gatccggtta gtacgccgtc atgacggcgg ccgcttcgca ccggcgcata tatgcagggt
     8221 ctcttgccgc gatccggtta gtacgtcgtt atgacggcgg ccgctgcaag atcggcgggt
     8281 acatgtgcag tgcctcttgg cgcgatcggg ttagtacggc gttatgacgg cgccgcgcag
     8341 tcgctctctt cggctcgagt gcgttcaaca tcgcgagcgt actaacgctg tactaaccag
     8401 attgcggtgg gacacccttc atgtataggg atctgctccc gaggcgctgc tcccgaggcg
     8461 ctgcgcccga ggcgctctct cccgaggcgc tctctcccga ggcgctctct cccaagctcg
     8521 ttgaggacgc gttgcgctga accgcggcgc tgacctagtc gcgctgcgcc cagaagttcg
     8581 ccatgaaccg cggcgctgaa ctagtcgcgc tgcgcccaga agttcgccat cacaggccac
     8641 aggcgatcgg ccgccttgcg cgacacgacc gcaccgacgt
//
LOCUS       DASUFX010000152         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_996786,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000152 DASUFX010000000
VERSION     DASUFX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996786"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1301)
                     /locus_tag="VFV99_06005"
     CDS             complement(150..1301)
                     /locus_tag="VFV99_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5058893.1"
                     /translation="MTREPHPPYRESALTKVLALLLLVVAGALVFTVIQQRRQTTQVV
                     NYEPKPIAQRPDDKLGASEQATIDVFEKFSRSVVHVTSLASRRNLMTLDVTEIPQGTG
                     SGFVWDQQGHIVTNFHVVQGGDRASVTLNDGSTYPATIIGTAPDKDIAVLHIDAPPTK
                     LLPLPVGQSSTLKVGMSVLAIGNPFGLDQTLTTGVISGLGREIKSVSGRPITDVIQTD
                     ASINPGNSGGPLLDSSGRLIGINTAIYSPSGANAGIGFAVPVDTVNAIVPQLLKHGKI
                     VRPGLGISIANDQQAARIGIDGVIVYQVSPNGAAANAGVQGVAIDPDSGGAIPQDVIV
                     GIDNVVIHKQNDLFKAMDAHKVGDVVDVQVDNKQTGKRRTVKVTLQAVQ"
     gene            complement(1407..2354)
                     /gene="trxB"
                     /locus_tag="VFV99_06010"
     CDS             complement(1407..2354)
                     /gene="trxB"
                     /locus_tag="VFV99_06010"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5058894.1"
                     /translation="MAENVVIIGSGPAAHTAAIYAARANLSPVLFEGFLAGGIAAGGQ
                     LTTTTDVENFPGFPDGIMGPELCERFRAQSARFGTRIFTETVTKLDLSSRPFKYETDE
                     QKGEAKTIIIATGATAKRDDVPGTRDGELWQRGVSACAVCDGALPMFRQKPLFVVGGG
                     DSAMEEANFLTKFASKVYIVHRRDELRASAIMQDRAKKNDKIEFLLSHKVVAVEGGNS
                     VELVKVHDLKTSQERSIPAAGLFFAIGHVPNTAFLGGQVKCDEQGYVLTTPGTTHTSV
                     PGVFAAGDVQDKKYRQAITAAGTGCMAALEAEHFLAAHH"
     gene            2429..3205
                     /locus_tag="VFV99_06015"
     CDS             2429..3205
                     /locus_tag="VFV99_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5058895.1"
                     /translation="MSLASLDQLDRSHRRHDDVMTQLLDAARRLAAGRPHDTDVNVVR
                     DAVAYFERAVTRHFLDEEGSVFPRLSTRRPELAEALASLSAEHPAQIALQNAVAECVK
                     QLDDESRPGAGKALLEIAEKLADQHRKHIEREDEIFRTAHEALTEQDDTDIVAEMETR
                     RDRDSEDSGRIRAGGGGGGGGGGRGRGGGGGGGGGQKPAAAAKPASKPAKKTVAKAKP
                     AAKSAKTTKQAAKTARSASKPKTSAKKSAKPAKKSAKKKR"
     gene            complement(3508..3759)
                     /locus_tag="VFV99_06020"
     CDS             complement(3508..3759)
                     /locus_tag="VFV99_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058896.1"
                     /translation="MDALDARLDRLETRLAASDRFADQQAEKQSDGFARVHSKFDESE
                     ERAKARSARIEARLDAIDGRLTTLTATVALHDLQLREAK"
BASE COUNT          666 a         1224 c         1267 g          680 t
ORIGIN      
        1 gcgcgcgatc gacgacagcc cgccgaaaaa cgcagtcgcc gcacccgagc ggacgcggcg
       61 aaagatggtg tcgcgataga agcgagtccg tgacggcgca gcaggctgtg taagcgaatc
      121 aaccgaggcg actgcgcggg cgcggcgaat cactggactg cttgcaatgt taccttgacc
      181 gtgcggcgtt tgcctgtctg cttgttgtcg acctgcacgt ccacgacgtc gccaaccttg
      241 tgggcgtcca tcgccttgaa tagatcgttc tgcttgtgaa tcacgacgtt gtcgatgccg
      301 acgatgacgt cctgcgggat cgctccgccg ctgtcgggat cgatcgcgac accctgcacg
      361 cccgcgttcg cggcggcacc attgggtgac acctggtaga cgatgacgcc gtcgatgccg
      421 atgcgcgcgg cctgctggtc gttcgcgatg ctgatgccga ggccggggcg cacgatcttt
      481 ccgtgcttga gcagctgcgg aacgatcgcg ttgaccgtgt cgacgggcac cgcgaagccg
      541 atgccggcgt tcgcgccact cggcgagtag atcgcggtgt tgataccgat gagccggccc
      601 gagctgtcga gcagcggacc gcccgagtta ccggggttga tcgatgcgtc ggtctggatc
      661 acgtcggtga tcggccggcc cgacacgctc ttgatctcgc ggccgaggcc cgagatcacg
      721 ccggtcgtga gcgtttgatc gagaccgaac gggttgccga tcgcgagcac gctcatgccg
      781 accttcagcg tcgacgactg gccgaccggc agcggcaaca gcttcgtcgg tggcgcgtcg
      841 atgtggagca ctgcgatgtc cttgtcgggc gcagtgccga tgatcgtcgc cggatacgtc
      901 gagccgtcgt tgagcgtgac gctcgcgcga tcgccgccct gcacgacgtg gaagttcgtc
      961 acgatgtggc cctgctgatc ccacacgaag ccggagcccg tgccttgcgg gatctcggtc
     1021 acgtcgagcg tcatcaagtt gcgccgcgac gcgaggctcg tgacgtgaac gaccgagcgc
     1081 gagaatttct cgaacacgtc gatcgtcgcc tgttcgctag cgccgagctt gtcatcgggc
     1141 cgctgcgcga tcggctttgg ctcgtagttg acgacttgcg tggtctgtcg tcgctgctgg
     1201 atgacggtga acaccagcgc gccggcaacg accagcaata gcaacgcgag taccttggtg
     1261 agggcagact cgcgatacgg cggatgaggc tctcgagtca cgggatagac cgattgtgac
     1321 atggcacctg ccgggtcagc acactcggca taccgggagc cgtggtccga tattcccgtg
     1381 gcactccgtg ccacttcggc acgcgcctag tggtgcgccg ccaaaaagtg ctcggcttcg
     1441 agcgctgcca tgcagccggt gccagccgcg gtgatcgcct gacggtactt cttgtcctga
     1501 acgtcgcctg cggcgaacac gcccggcacg ctcgtgtgcg tagtgcccgg cgtggtcagg
     1561 acgtagccct gctcgtcgca cttcacctgg ccgccgagga acgcggtgtt gggcacgtgg
     1621 ccgatcgcga agaacagacc ggcagccgga atcgaccgct cctggctcgt cttcagatcg
     1681 tgcaccttca cgagctcgac gctgttgccg ccttcgaccg cgacgacctt gtgggagagc
     1741 aggaactcga tcttgtcgtt cttctttgcg cggtcctgca tgatcgccga cgcgcgcagc
     1801 tcgtcgcggc ggtgcacgat gtagaccttc gacgcgaact tggtcaggaa gtttgcctct
     1861 tccatcgccg agtcgccgcc gccgaccacg aacagcggct tttggcggaa catcggcaac
     1921 gcgccatcgc acaccgcgca cgccgagacg ccgcgctgcc acagctcgcc atcgcgcgtg
     1981 cccggaacgt cgtcgcgctt cgcggtcgca ccggtcgcga tgatgatcgt cttcgcttcg
     2041 cccttctgct cgtccgtctc gtacttgaac gggcgcgacg acagatcgag cttggtcacc
     2101 gtctcggtga agatgcgcgt accgaaccgc gccgattgcg cgcgaaaccg ctcgcacagc
     2161 tcggggccca tgatcccgtc cgggaagccc ggaaagttct cgacgtcggt ggtcgtcgtg
     2221 agctgaccgc cggcagcgat cccgcctgcc aggaagcctt cgaacagcac aggactgagg
     2281 ttcgctcgcg cagcgtaaat ggcagctgta tgcgctgcgg gcccgcttcc gatgatgacg
     2341 acgttctcgg ccatgcgcct gtgtaccagc gttttttcgc cgaggacagt ggtcccgcac
     2401 cgcactggtg tggatcatgg ggccccccat gtcactggcc agcctcgacc agctcgaccg
     2461 ctcgcaccgg cgtcacgacg atgtgatgac gcagctgctc gatgcggcac gacgcctcgc
     2521 ggcaggccgc ccgcacgaca ccgatgtgaa cgtggtccgc gatgcggttg cgtacttcga
     2581 gcgcgcggtc acccggcact ttctcgacga ggaaggctcg gtgtttcccc ggctgtcgac
     2641 gcgccgtccc gagctggccg aagcgctagc tagtctttcg gcggagcacc ccgcgcagat
     2701 cgcattgcaa aacgcagtcg cagaatgcgt gaagcagctc gatgacgaat cacgaccggg
     2761 tgccggcaag gcgctgctcg agatcgccga gaagctcgcc gaccagcatc gcaagcacat
     2821 cgagcgcgag gacgagatct ttcgcaccgc acatgaagcg ctgaccgagc aggacgacac
     2881 cgacatcgtt gccgagatgg agacgcgccg cgatcgcgac agcgaagaca gcggccgcat
     2941 ccgagccggt ggtggtggtg gtggcggcgg cggtggccga ggtcgtggcg gtggcggtgg
     3001 cggcggtggc ggccagaaac cggctgcagc cgcgaagccg gcgagcaagc ccgcgaagaa
     3061 gaccgtcgcg aaagcgaagc cggccgcgaa gagcgcgaag acgacgaagc aagcggcaaa
     3121 gaccgcgcgc tcggcgagca agcccaagac gagcgcaaag aagtctgcga agccggcgaa
     3181 gaagtcagcg aagaaaaagc gctgagcggt cgtcgtagta tcgaagagcg gatcgacact
     3241 cggcatggaa tcgcgtgagc tgcgttccga tccgaccgat acgctcgagc ttctcgagga
     3301 tctggagctg gacatgcgtg tagaccacac gtggcttcag tggtttcggt ggaggcggcg
     3361 cgatcggacc gctccagtca ctcgcccgca accgagattg catcgcaacg aggttagcct
     3421 cgatgtcgcg gagctgatcg ccaagccgaa atgcgagcaa tgtccatcga acttcgtggc
     3481 gccacagctc gtcggcgtct tcgtgggtca cttcgcctct ctgagttgga ggtcgtgcag
     3541 cgcgacggtc gccgtgagtg tggtcagacg accgtcgatt gcatcgagac gcgcttcgat
     3601 gcgcgcggag cgcgccttgg cccgttcttc gctctcgtca aacttgctgt gcacgcgcgc
     3661 gaaaccatcc gactgttttt cagcctgctg gtcggcaaac cgatcgctcg ccgcaagcct
     3721 cgtttcgagt cggtcgagcc gcgcatcgag cgcatccatt cgccccgaga cgttcgcgtc
     3781 gagcttgacg accaggtccc tggttgcgtc gttcgacttc ttcatgtcga agatgta
//
LOCUS       DASUFX010000153        17417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_718475,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000153 DASUFX010000000
VERSION     DASUFX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718475"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFV99_06025"
     CDS             complement(<1..926)
                     /locus_tag="VFV99_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX5058897.1"
                     /translation="MRENSRELELLFEELLIGVTSFFRDPGEWERLRQEVLPTLVNAR
                     ASTGLLRAWVAGCSTGEEAYSLAILFKEAIETIRPHKNIAVQIFATDLDREAIEKARV
                     GLYPENVAADVSPERLRRFFVREDRGYRVSKDIRESVVFAPQNVIMDPPFTKVDIVAC
                     RNLLIYLSAEVQRKLIPLFHYCLNPGGVLFLGTAETVGSFSNMFAPLDGRTRLYKRLD
                     QTVGAMPMDLQFTTFGRGEPARGEREDAPPQAKQVTPNVQTLVDRLLVQRFAPLGVLC
                     NTKGDVLYLSGRAGKYFEPAVGKANLNIVAMAR"
     gene            complement(943..1836)
                     /locus_tag="VFV99_06030"
     CDS             complement(943..1836)
                     /locus_tag="VFV99_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5058898.1"
                     /translation="MPMATKKTKPTSKGKPKNGKPKNGKARTLSAASVLPVENPSLPT
                     SGHEHQHAASFPIIGIGASAGGLEALEVFLARVPRQAGAAFVIVQHLDPQYKGMLVEL
                     LQRVTQLQVIEAGDGTPVQPEHVYVIPPGKDMSLLHGSLHLLPQASARGRVLPIDFFF
                     RSLAQDQGERSIGVILSGMGTDGTLGLRAVKEAAGATFVQSPDSAKFDSMPRSAIDAG
                     LADVVAAVEELPARILSYRDHAPFISSHDEMRVTDAQSAVEKICVLLREYSGNDFTVY
                     KRSTIYRRIERRMASSPDPQL"
     gene            complement(1887..3554)
                     /gene="treZ"
                     /locus_tag="VFV99_06035"
     CDS             complement(1887..3554)
                     /gene="treZ"
                     /locus_tag="VFV99_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX5058899.1"
                     /translation="MNRSLRVWAPDAHRVDVVVGAEEQTAARESHGWWRGPRLHEGDL
                     YWIRIDGGPPRPDPRSTFQPQGVHGPSCWLDTATLGAPGPAPYVSLRDAVIYELHVGT
                     FTQAGTFSSAVEHLDDLVELGVTHVELMPIAQFPGVHGWGYDGVDLYAAHTPYGGPPG
                     LRDLIAECHARGLAVIIDVVHNHFGPEGAYLAGIAPYKTSHHITPWGDAINLDDVGSD
                     EVRRFLIDSAIAWLRDFGADGLRLDALHALYDDSEHHFVEQLVDEVRVLERDLDRRFV
                     LIGEYDHHDPKVLIEWGLDAHWNDDFHHAVHALVTGEHDSYYVDFVGKLGTVLEHGYA
                     LDGGFSKFRGERHGRPFGALSRDRLVAYVQSHDQVGNRPRGERLHQLAGFKRAQLAAA
                     LLLTSPFVPMLFQGEEWAASTPFLYFAQLESEQLRRAVREGRRKEHRHDGVTPPDPEA
                     PSTRDASVLRWRERGEGEHAQMLAWYRTLIAARRAHPALRDPSPEATRVEEHGGLLTV
                     HRDDLALVCNFTKEPRRMLAGELLVASSGSHAAHELSGLACALVRRR"
     gene            3748..5004
                     /locus_tag="VFV99_06040"
     CDS             3748..5004
                     /locus_tag="VFV99_06040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058900.1"
                     /translation="METVGGRYALDGRLGEGGMGHVYRARHIQLGKAFALKIISPAFA
                     ADNAARVRFNQEAKLASEISHPNIVSVVDFGEDPQFGAYMVMELVEGEPLIAPGVLPM
                     SVKRAIDVLGQVADALDHIHKRGIVHGDVKAENIMLTAETTSQSGGARRRRIVRLLDF
                     GLARRPDHAEEEGLSGSPHYLAPERAAGGPASVSSDVYALGVLAYLLLTGTLPYEGGV
                     VEVLMAQINNTPEPISVRRREEVDPALESLILRAMAKDPAQRHGSAAAFRYELNTVMD
                     MLDMGRRRPRGSGAIPPENARETSITQAFDRSRIPQAILSVEGLFVATNRAFNKLVNQ
                     DEKGCDGLILSDTTLSTFVPGLMRALRTVHVDGKPVERRAKVFRGNDRPPYELSIWLS
                     PLPIPGQEIHMLVRVEEVDPRREHDR"
     gene            5022..5864
                     /locus_tag="VFV99_06045"
     CDS             5022..5864
                     /locus_tag="VFV99_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5058901.1"
                     /translation="MRGVVAVPWLALSACSFQHGVSVGDASPPDTQRSDADIDAPNEG
                     LVSRNLVARYFIDEAAAGQSPTQLTDAAANPFPLAMTYTGTLSFTEVATQRGLHWTTP
                     GDLGRASAPIDNTKITMALAGAKRWTYELVVDLRNATSGECRLISIGNGTATYGDAAL
                     ITSDLTTLRLHVDATYAHWNVTFGSGRVVLHAVVDTTLATSTSRYQLFVNGTQALRIA
                     GTVPVQDAGLVIPANDYLAIGNVEGAGRSPGGDVYYAAIYSDALTPAEITANTARLLA
                     NDDH"
     gene            complement(5902..6981)
                     /locus_tag="VFV99_06050"
     CDS             complement(5902..6981)
                     /locus_tag="VFV99_06050"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5058902.1"
                     /translation="MKLLAGALAIVAITGCKKKETKPTTEQGSGSAAEEVKMPRASQG
                     VLPQMAALELPADPKRDAKVALGHQLFFDKRLSGASDRACYSCHMNEDGNGGHDPLAI
                     GSGDKPLTRHSPVIWNVGYWKNAFYWDGRAPTLEDNVKGAWGGPNMNAGKDNLDKKAA
                     ELAALPSYKKLFADAYDKEPIKGEQVAQALAEYMRTLVCKDTAYDKFAAGDKAALNEQ
                     QQRGLDVFAGKGACITCHAPPYFSTAMGVDGGIYFNAGIGTGVAEDKVDIGRMKVTND
                     PKDWAAFKPPSLRNVTKSAPYFHDGSVAKLEDAVKIMSTGGVKNKNLNPALADRGLSD
                     AERADLVAFLGALECPGTLEVVEPK"
     gene            complement(6987..7439)
                     /locus_tag="VFV99_06055"
     CDS             complement(6987..7439)
                     /locus_tag="VFV99_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058903.1"
                     /translation="MKQLGQGRVSLMMKNLIFVVALAACGHKGSTPIANEGSGAGSGS
                     SAPVGPADTRTELEKRRDGACETLGPKITQCAVEDAKRELAAGKVKQKDFDANTAPDV
                     LRKNTDEFIKSCEKPQYSSRQVRVLEVCQREETQCEPLLACLENLNKK"
     gene            complement(7436..7792)
                     /locus_tag="VFV99_06060"
     CDS             complement(7436..7792)
                     /locus_tag="VFV99_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058904.1"
                     /translation="MKVWALAIALGACSSTHGTGTGSGGSGLGSAPPVANAKTCDDVK
                     ARVEQMYRGEAQVKEPKRVDEAVADNTAMVMKDCAKDPDKTVPCLARAGSVAELEKQC
                     LIQLDDEGTEGEALAK"
     gene            complement(7816..8580)
                     /gene="surE"
                     /locus_tag="VFV99_06065"
     CDS             complement(7816..8580)
                     /gene="surE"
                     /locus_tag="VFV99_06065"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5058905.1"
                     /translation="MPRPLVLCSNDDGVHSPHLEALAQRIEEFADVIVVAPERQRSAA
                     SHAITLHKPLRLTEVKPRRYSLSGTPVDCVYLGVLKLCDRKPAVVVSGINDGYNLGSD
                     VFYSGTVAAAVEGALRGAAGIACSLAPRAKDVDHAVDFAARVVRAAINEPIPSGTVLN
                     VNMPGTGSQNYQWTTLGRRLYEDDVDERKDPRGRSYYWVGGGPAGHDHVEGTDCVCVS
                     RGDNSITPMHLDLTDRPRIARPPWEIEGFRVVVGPS"
     gene            complement(8652..8725)
                     /locus_tag="VFV99_06070"
     tRNA            complement(8652..8725)
                     /locus_tag="VFV99_06070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8689..8691),aa:Pro,seq:ggg)
     gene            complement(8803..9102)
                     /locus_tag="VFV99_06075"
     CDS             complement(8803..9102)
                     /locus_tag="VFV99_06075"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX5058906.1"
                     /translation="MARMTKADIIESVYEKVGGFSKKEAAEIVETVFNVVKETLERGE
                     KIKISGFGNFVVRDKKDRMGRDPQRAVPILIPARRVLTFKPSQVLKNILNGLPPV"
     gene            complement(9140..9946)
                     /locus_tag="VFV99_06080"
     CDS             complement(9140..9946)
                     /locus_tag="VFV99_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058907.1"
                     /translation="MSVPFGRWVWIDRLPDEPILAFVYLDQHAGPSAKGGLASDPNVA
                     TMPSRTVRLAGIEVRELGAHEVAERKLPARPPWLDHYGPQPDAEAPWRTDPLLEGKFH
                     PQFPDDAQVLVHDGDPRRTRRGPELCWVRIQRVARAPTRQLLHPSAIEAPETAGRPPS
                     TIEPSRHVYIGELLNQPHTLQSVKKGDRLKVISVHGMPHPLHVTDEYLAEREDWAISP
                     CHKCGATECFDPPSVMARFRFPDAPPDAVFEAFTSFCSLCGGMQMLKHLA"
     gene            complement(10005..12446)
                     /gene="pheT"
                     /locus_tag="VFV99_06085"
     CDS             complement(10005..12446)
                     /gene="pheT"
                     /locus_tag="VFV99_06085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5058908.1"
                     /translation="MKVLWSWLLELCDLDNVPTVEEGARALTRGGIEIEGMTNLGAGF
                     KGVVVAEVVGKRPHPQADKLTLVDVITERGGAATQVVCGAPNVPAPGRKVLWAQIGAE
                     LPGGLKLAAKAVKGIESPGMLCAEDELGLSEDHAGIVVLDADDATPLGAPAQRALGID
                     DWLLEVNAPANRGDLNGHIGVARELVAMLRGKLVLPDADLSAYAAGGNAKVEIAIASG
                     EDCPRYVARVIDGLTVKPSPRRIAQRLRAVGVRPISNLVDVTNYIMFELGQPLHAFDA
                     AKLTTGIVGVSPAADGEKFTTLDNQQRTLVKGDLMIRDGDRGIALAGVMGGADTEVTD
                     GTTRVLLESASFRPLSVRKTARRLGLHSEASHRFERGVDPELAGFASARATRLLCMVG
                     GGKVIGDAVDAYPGKREIAPIPTRLPRVQMLTGVPLTETACRDALERLGFTVQSTQSE
                     LTVTPPSPRSDVVREVDVIEEILRVTGYEQVPSTLPVMRQAPQTREANRADVARRALA
                     AAGAAEAITYGFQSVARVDALGLAATDRRAQPIIIRNPMSIDQAAMRTSLLPNLIAAI
                     ARNQSFGRGDVALFEVGSVFLRRGERVTEGAMHELADEPTWAAGVLAGNRPGQIGDGA
                     PWDAFDAKALALEAIRAVAGEVRVRANPVSNVSYLHPGVAGELVAELHDGKLVVGWFG
                     EVHPDTREKLGVTGPAFAFEVELDKLPLAQPAQMRTIPRFPGSERDVSLLLAEDILAA
                     RVVDVIEAANEPLVSRVRLLEDYRDAKLPAGHKSMLWSIAYRSPERTLTDAEVDKAHE
                     TIVGRLVENLPAQRR"
     gene            complement(12446..13477)
                     /gene="pheS"
                     /locus_tag="VFV99_06090"
     CDS             complement(12446..13477)
                     /gene="pheS"
                     /locus_tag="VFV99_06090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5058909.1"
                     /translation="MSTTTDLIAKLEQLGDEFLAQAQALADEQSIRAAQAQFLGKKGK
                     VSELMKELGKLPAGDRPQVGAAANKVKEKIEQIVDASLSRLTNAKKQADLARKVDITL
                     PARPVGGGHLHLLSQVRLEAISVFAELGFVVAEGPQIETDWHTFEALAIPKDHPARDM
                     QDTFYVSEEIVLRTHTSPVQVRTMLTQPPPIRIVAPGHVYRRDDDPTHSPMFTQIEGL
                     AVDENISFADLKGTLLHFVKRFFMKDLGIRLRPSYFPFVEPGAEVDMQCSFCGGSGCR
                     ICKHTGWVEIGGAGMVDPDVFKNCGIDGERYTGFAFGMGLERMAMLRHGVNDIKYYYE
                     GDIRFLEQF"
     gene            complement(13535..13873)
                     /gene="rplT"
                     /locus_tag="VFV99_06095"
     CDS             complement(13535..13873)
                     /gene="rplT"
                     /locus_tag="VFV99_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5058910.1"
                     /translation="MPRAKRGFKARRRRNRVLKWAKGFRGKRKNCHAIANESVQHAWM
                     HMYRSRKVKKRDFRALWIQRINAAARNLGISYSKLMGKLSGGNVQLNRKMLAEIAISD
                     PVAFEKILNA"
     gene            complement(13889..14086)
                     /gene="rpmI"
                     /locus_tag="VFV99_06100"
     CDS             complement(13889..14086)
                     /gene="rpmI"
                     /locus_tag="VFV99_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5058911.1"
                     /translation="MPKVKTHSGAKKRFKKTASGKFKFMRVFKRHLLTGRSKKRKRQL
                     RNASYVPSAHHHQIDVLLPYA"
     gene            complement(14417..15151)
                     /gene="infC"
                     /locus_tag="VFV99_06105"
     CDS             complement(14417..15151)
                     /gene="infC"
                     /locus_tag="VFV99_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5058912.1"
                     /translation="MNVRPPGGRFDRRRPEQGLRINHRIRVPEVRVIADEEQLGVMPT
                     HEALRLAEEKGLDLVEISPRAFPPVCRIMDYGKYKYEEAKKKQQARKRASTVETKEIK
                     FRPKTEEHDMAFKVKHVRRFLEGGNKVRLAVVFRGREITHPQTGMNVLNKIVDLCNDI
                     AVVEATPNMEGRRMIMVIAPKPGVVRKAQEAKKAQVAAQMAAEAAKRNQQPGKPSAAP
                     PPEPDEDEDLDEDEINADVEDDDDEE"
     gene            complement(15310..15855)
                     /locus_tag="VFV99_06110"
     CDS             complement(15310..15855)
                     /locus_tag="VFV99_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5058913.1"
                     /translation="MRAVVLEMPPHWLEERRRLGHDKRDEVWDGVLHMVPQPGTTHQG
                     LEVELIYALKALVEARGYRLFVETSLFGAAGEKNYRVPDLSVVAPSDFSERGLEQHAE
                     IVVELLSPNDESRDKLPFYAMCGVGEVWLIEPKTRTIEVLSLDDAYGSTRTESAVLGI
                     RFSTVDGPKLRLDWQGGSAEV"
     gene            complement(16027..16098)
                     /locus_tag="VFV99_06115"
     tRNA            complement(16027..16098)
                     /locus_tag="VFV99_06115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16064..16066),aa:Val,seq:gac)
     gene            16230..>17417
                     /locus_tag="VFV99_06120"
     CDS             16230..>17417
                     /locus_tag="VFV99_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058914.1"
                     /translation="MQVERWSLPRVVAFRFGVLAALLLVSPTVLWPVPGAAPLVIAIM
                     RGWHWLATQFGALLGLDVPPLAPTGSGDALWNWLQALLSILLAVPGTLVWLLVDRRRA
                     HPRLAAAMTVFLRYYLAAVLIGYGMAKVVPMQFAPLPLARYDTTLGEMSPMGLMWTFM
                     AFSQPYVFFAGIAEVIGSVLLLSRRTYVAGALLLIVVMANVVVMNFCYDVPVKLFSML
                     LIVLLCALVAPHAGRLIGALLGYPTREVPPRVRGSVMTERVRLALKLAMVAVIAVRAY
                     EHVVISRWVEEQKTITALHGTWRAERVVIDGIERPPLFTDDARWRKLIFHEWGLTVRF
                     ATDRRLNFATQVDATAQTINVGQGPFRQVWRYRLVDSEHLIIDTPTVRGELVLEPEPP
                     LTTR"
BASE COUNT         2807 a         5865 c         5620 g         3125 t
ORIGIN      
        1 cgcgccatcg ccacgatgtt gaggttcgcc ttaccgacag ccggttcgaa gtacttcccg
       61 gcgcggccgc tgaggtagag aacatcgccc ttggtattgc agagcacgcc gagcggcgcg
      121 aaccgttgca cgagcaaccg atcgaccaac gtctgcacgt tgggcgtgac ctgcttggcc
      181 tgcggaggtg cgtcctcgcg ctctccgcgc gccggttcac cgcgaccaaa cgtcgtgaac
      241 tgcagatcca tcggcatcgc gccgacggtc tgatcaagcc gcttgtacag ccgcgtccgg
      301 ccgtcgagcg gcgcgaacat gttgctaaac gacccgacgg tctcggcggt cccgaggaag
      361 agcacgccac cgggattcag acagtagtgg aacagcggga tcagcttacg ttggacttcc
      421 gccgacaggt agatcaacaa attccggcac gcgacgatgt ccaccttcgt gaacggcgga
      481 tccatgatca cgttctgcgg cgcaaacacg acggactcgc ggatgtcctt gctcacgcga
      541 taaccgcgat cctcccggac gaagaaccgc cgcagccgtt cgggcgatac gtcggccgcg
      601 acgttctcgg gatacaaccc gacgcgcgct ttttcgatcg cctcgcgatc gaggtcggtc
      661 gcgaagatct ggaccgcgat gttcttgtgc gggcggatcg tctcgatcgc ttccttgaac
      721 aggatcgcga gcgagtacgc ctcctcgccc gtcgagcaac cggcgaccca cgcgcgcaga
      781 agacccgtcg acgcgcgcgc attcacgagt gtgggcaaga cctcctgacg cagccgctcc
      841 cattcgcccg gatctctgaa aaaactcgtg accccgatca ggagctcctc gaacaacagt
      901 tcgagctcgc ggctgttctc gcgcacgtag cgcaggtagt cgctaaagct gcggatctgg
      961 tgaagacgcc atccggcgct cgatgcgccg atagatcgtg cttcgcttgt aaaccgtgaa
     1021 gtcgttgccc gaatactcgc gcagcagcac gcaaatcttt tcgaccgcac tttgcgcgtc
     1081 ggtgacccgc atctcgtcgt ggctgctgat gaacggggcg tggtctcggt acgacaagat
     1141 tcgcgcgggc agctcctcga ccgccgcgac cacgtcagcc aggcccgcgt cgatcgcgct
     1201 gcggggcatg ctgtcgaact tcgccgagtc cggcgattgg acaaacgtcg caccagccgc
     1261 ctctttcacg gcgcgcagac cgagcgtgcc gtcggtaccc atgcccgata agatgacgcc
     1321 gatgctgcgc tcgccttgat cctgagcgag cgagcgaaag aagaagtcga ttggcaagac
     1381 gcgaccgcgc gcggacgcct gcggtagcag gtgaagcgaa ccatgcagca gcgacatgtc
     1441 cttgcccggc ggaatcacgt agacatgctc gggctgtacc ggcgtgccgt caccagcctc
     1501 gatcacctgg agctgggtga cccgctgcaa cagctcgacg agcattcctt tatattgcgg
     1561 gtcgagatgc tggacaatca cgaaggcggc gccggcctgg cgcggcacgc gcgcgaggaa
     1621 cacctcgagc gcttcgaggc cacccgcgga cgcaccgatg ccgatgatcg gaaagctcgc
     1681 cgcgtgctga tgctcgtgcc cggacgtcgg cagcgacgga ttctcgaccg gcaagaccga
     1741 ggccgcagag agcgtccgcg ccttgccgtt cttgggcttg ccgttcttgg gcttgccctt
     1801 gctcgtcggc ttcgtcttct tggttgccat cggcatggga tgcgtaacac aggcacgttg
     1861 caggcgcagt gcacccttcc gatctctcag cgtctccgca cgagggcaca cgcgaggccc
     1921 gagagctcgt gtgccgcatg gctcccactc gacgcgacga gcagctcgcc ggcgagcatg
     1981 cggcgtggct ccttcgtgaa gttacagacc aacgcgaggt catcacggtg cacagtcaag
     2041 aggccgccgt gctcctcgac gcgcgtcgcc tcgggtgacg gatcgcgaag cgccgggtga
     2101 gcacgccgcg ccgcgatcaa cgtgcggtac caggcgagca tctgcgcgtg ctcaccctcg
     2161 ccacgttcgc gccagcgcaa caccgaggcg tcgcgcgtgg aaggcgcctc ggggtccggt
     2221 ggcgtcacgc cgtcgtggcg gtgttctttg cgccgtcctt cgcgcacggc ccgccgcagc
     2281 tgttcggact ccagctgggc gaagtagaga aacggcgtcg aggccgccca ttcctcgccc
     2341 tggaacagca tcggcacgaa cggcgacgtc agcagcagcg ccgctgcgag ctgggcgcgc
     2401 ttgaaaccgg cgagctggtg gagacgttcg ccgcggggac gattaccaac ctggtcgtgc
     2461 gactgcacgt acgcgacgag tcgatcgcgt gacagcgcgc cgaacggccg gccgtgacgc
     2521 tcgccgcgaa acttcgagaa cccgccgtcg agcgcatagc catgttcgag caccgtgccg
     2581 agcttgccga cgaagtcgac gtagtagctg tcgtgctcgc cggtgacgag cgcgtgcacg
     2641 gcgtggtgaa agtcgtcgtt ccagtgcgcg tcgagtcccc actcgatcaa caccttcgga
     2701 tcgtgatggt cgtactcgcc gatcagcacg aaccggcgat cgagatcgcg ctctagcacg
     2761 cgcacctcgt cgacgagctg ctcgacaaag tgatgctcgc tatcgtcgta aagcgcgtgc
     2821 agcgcatcga gccgcaggcc atcggcgccg aagtcacgca gccatgcgat cgcgctgtcg
     2881 atcaagaacc gccgcacctc gtcgctgccg acgtcatcga ggttgatcgc atcaccccac
     2941 ggcgtgatgt ggtggctcgt cttgtagggc gcgatgccgg cgaggtacgc gccctcgggt
     3001 ccgaagtggt tgtgaacgac atcgatgatc accgcgagcc cgcgcgcgtg acattcggcg
     3061 atcaaatcgc gcagcccggg cggcccgcca tacggagtgt gcgccgcata cagatcgacg
     3121 ccgtcgtagc cccaaccatg cacgcctggg aactgcgcga tcggcatcag ctcgacgtgc
     3181 gtgacgccga gctcgacaag gtcgtcgagg tgctcgactg cggagctgaa cgtaccggcc
     3241 tgcgtgaacg taccgacatg cagctcgtag atgacggcgt cgcgcaggga cacgtacggc
     3301 gcaggcccgg gcgcaccgag cgtcgccgtg tcgagccagc acgatggccc gtgcacgccc
     3361 tgtggctgaa acgtcgagcg cggatcgggc cgcggcggtc cgccatcgat ccggatccag
     3421 tacaagtcgc cttcgtgcag cctcggtcca cgccaccaac cgtgcgactc gcgcgcggcg
     3481 gtctgctcct cggctccgac gacgacatcg acgcgatggg cgtcgggggc ccagacgcga
     3541 agcgaccggt tcatggctcg gtgcgatgcc atccgtgtgc cgctgagccg gcggattgcg
     3601 cagcacaaac cgtgcgatgg cgccgcagac tctgcgcggt acgagcgttt cgcaggcacg
     3661 agaaaggtcg taccggtgcc gatcacccag gactgttgaa gtcacaagtg gatatcgctt
     3721 gtgagctggc tttatattct tgctgcaatg gaaacggtgg gtggtcgtta cgccctagac
     3781 ggtcggctcg gtgaaggagg gatgggacac gtctatcgcg caaggcatat ccagctcggc
     3841 aaagcgttcg cgctgaagat catctcgccg gcgttcgctg ccgataacgc tgcgcgcgtg
     3901 cgcttcaatc aagaagcgaa gctcgcgagc gaaatctcgc atccgaacat cgtctccgtc
     3961 gtcgacttcg gcgaggaccc acagtttggc gcgtacatgg tgatggagct cgtcgagggc
     4021 gagccgctca tcgcgcccgg tgtgctgccg atgtcggtca agcgcgcgat cgacgtgctc
     4081 ggccaggtgg cggacgcgct cgatcacatt cacaaacgcg gcatcgtgca cggcgacgtc
     4141 aaagccgaga acatcatgct caccgccgag acgacgtcgc agtcgggcgg tgcgcgccgc
     4201 cgccgcatcg tgcgcttgct cgacttcgga ctcgcgcgcc gtcccgatca cgccgaggag
     4261 gaaggtctga gcggcagccc gcattacctc gctcccgagc gcgcagcggg cggtcccgca
     4321 tcggtctcgt cggacgtgta cgcgctcggc gtgctcgctt atctgctgct caccggcacg
     4381 ctgccctacg aaggcggtgt cgtcgaagtg ttgatggcgc agatcaacaa cacgccggag
     4441 ccgatttcgg tgcgtcgccg cgaagaagtc gacccggcgc tcgagagctt gatcctgcgc
     4501 gcgatggcga aggatccggc gcaacgtcac ggcagtgccg cggcgttccg ctacgagctc
     4561 aacacggtga tggacatgct cgacatgggc cgtcgccgtc cgcgcggtag tggcgcgatt
     4621 ccgcccgaga acgcgcgcga aacctcgatc acgcaggcat tcgatcgctc acgcattccg
     4681 caagcaattc tctccgtcga gggcttgttc gtcgcgacga accgcgcgtt caacaagctc
     4741 gtcaaccagg acgagaaggg atgcgacggc ctcattctct ccgacaccac gctgtcgacg
     4801 ttcgtgcccg gcctcatgcg cgcgctgcgc acggtgcacg tcgacggcaa gccggtcgag
     4861 cgccgcgcga aagtgttccg cggcaacgat cgcccgccgt acgagctatc gatttggctg
     4921 tcgccgctgc cgattcccgg ccaagaaatt cacatgctcg tgcgcgtcga agaggtcgat
     4981 ccgcgccgcg agcacgaccg ctagtcgcga tacgataagg cgtgcgaggc gtcgtggccg
     5041 taccgtggct cgctctgtcg gcgtgctcgt tccagcacgg cgtcagcgtg ggcgatgcgt
     5101 cgccgcccga cacgcaacgt agcgacgccg acatcgacgc gccgaacgag ggcctggtct
     5161 cgcgcaacct ggtcgcacgt tacttcatcg acgaagccgc agcggggcag tcgccaacgc
     5221 agcttaccga tgcggccgcg aatccgttcc ccctcgcgat gacgtacacg ggcacgctgt
     5281 cgttcaccga ggtcgcgacc cagcgcgggc tgcactggac gacaccgggc gacctcggcc
     5341 gcgcgtcggc gccgatcgac aacacgaaga tcacgatggc gctcgccggc gccaagcgat
     5401 ggacgtacga gcttgtcgtc gaccttcgca acgcgacgtc cggcgagtgc cgcctgatct
     5461 cgatcggcaa tggcactgcg acctatggcg acgcggcgct gatcaccagc gacctcacga
     5521 cgctgcgcct ccacgtcgac gcgacgtatg cacattggaa cgtcacgttc ggttcgggcc
     5581 gtgtcgtatt gcacgccgtc gtcgacacca cgctcgcgac ctccacgagc cgctaccagc
     5641 tgttcgtcaa cggcacacag gcgctgcgca tcgcgggcac cgtgcccgtg caagacgccg
     5701 gcctcgtgat tccggccaac gactatctcg cgatcggcaa cgtcgaaggc gcaggccgct
     5761 cgcccggcgg cgacgtctac tacgccgcga tctactccga cgcgctcacg cccgccgaga
     5821 tcacggcgaa cacggcgcgc ctcctggcca acgacgacca ctgacggcgg aagcgttgcg
     5881 tgcgcggaat gtcgatccgg actacttcgg ctcgacgact tcgagcgtgc ccgggcactc
     5941 gagcgcaccg aggaacgcga ccaggtcggc gcgctcggca tcgctgagcc cacggtccgc
     6001 gagggcgggg ttcagattct tgttcttcac gccgcccgtc gacatgatct tcaccgcgtc
     6061 ctcgagcttg gcgaccgagc catcgtggaa gtacggcgcg ctcttcgtca cgttgcgcag
     6121 gctcggcggc ttgaacgctg cccagtcctt cggatcgttc gtgaccttca tgcggccgat
     6181 atcgaccttg tcctcggcga cacccgtgcc gatgccggcg ttgaagtaga tgccaccgtc
     6241 gacgcccatg gcggtcgaga agtacggcgg tgcgtggcac gtgatgcacg cgcccttgcc
     6301 cgcaaacacg tcgaggccgc gctgctgctg ctcgttcagc gccgctttgt cacctgcggc
     6361 gaacttgtcg tacgcggtgt ccttgcagac gagcgtacgc atgtactccg cgagcgcctg
     6421 cgcgacctgc tcgcctttga tcggctcctt gtcgtacgcg tccgcgaaca gcttcttgta
     6481 gctgggcagt gcggcgagct cggcggcctt cttgtcgagg ttgtctttgc ccgcgttcat
     6541 gttcggaccg ccccacgcgc ccttcacgtt gtcctcgagc gtcggcgcac gaccatccca
     6601 gtagaacgcg ttcttccagt agccgacgtt ccagatcacc ggcgagtgac gcgtcagcgg
     6661 cttgtcgccc gagccgatcg cgagcggatc gtgaccgccg ttgccatcct cgttcatgtg
     6721 gcacgagtag caggcgcgat cgctggcgcc cgacaaccgc ttgtcgaaga acagctggtg
     6781 accgagcgcg accttcgcgt cgcgcttcgg atcggcgggc aactccagcg ctgccatctg
     6841 cggcaacacg ccttgcgatg cgcgcggcat cttgacctct tcggcggccg agccactgcc
     6901 ctgctccgtc gtcggcttgg tttccttctt cttgcagcca gtgattgcaa cgatcgccag
     6961 cgcacccgcg aggagtttca tgatggtcac ttcttgttga ggttttcgag acacgcgagc
     7021 aacggctcgc actgcgtctc ttcacgttgg cagacctcga gcacgcgtac ctggcgcgac
     7081 gagtactgcg gcttctcgca tgacttgatg aattcgtcgg tgttcttgcg cagcacgtcc
     7141 ggcgccgtgt tcgcgtcgaa gtccttctgc ttcaccttgc cggcggcgag ctcgcgcttc
     7201 gcgtcctcga ccgcgcactg cgtgatcttc gggccgagcg tctcgcacgc gccatcgcgg
     7261 cgcttctcga gctcggtacg cgtatcggcc ggaccgaccg gcgcgctact gccgctacct
     7321 gccccgctgc cctcattggc gatcggcgtc gatcccttgt gcccgcacgc ggcgagcgcg
     7381 accacgaaaa tcaggttctt catcatcagc gagacacgtc cttggccgag ctgtttcatt
     7441 tggcgagcgc ctcgccttcg gtgccttcgt cgtcgagctg gatcaagcac tgcttctcga
     7501 gctcggccac cgagccggca cgtgcgaggc acggcacggt cttgtccgga tccttggcgc
     7561 aatccttcat caccatcgcg gtgttgtcgg cgaccgcttc gtcgacgcgc ttcggctctt
     7621 tgacctgcgc ctcgccgcga tacatctgct cgacccgcgc cttcacatcg tcgcaggtct
     7681 tcgcattcgc gaccggcggt gcgctgccaa ggccgctgcc gccgctaccg gttccggtgc
     7741 cgtgcgtgct cgagcacgcg ccgagcgcga tggccaaggc ccacaccttc atcgcgcgga
     7801 gcctaacgcg gtctgttagc tcggtccaac gactacgcga aagccttcga tctcccacgg
     7861 cgggcgcgca attcgcggcc gatcggtcag atcgaggtgc atcggcgtga tgctgttgtc
     7921 gccacgcgag acacagacgc agtcggtgcc ttcgacgtgg tcgtgtcccg caggaccgcc
     7981 gcccacccag taatacgagc gaccgcgcgg atccttacgc tcgtcgacgt catcctcgta
     8041 gagccggcgc ccgagcgtcg tccactgata gttctggctg cccgtgccgg gcatgttcac
     8101 gttgaggacc gtgcccgacg gaatcggctc gttgatcgcg gcgcgcacga cgcgtgcggc
     8161 gaagtcgacc gcgtgatcca cgtccttcgc acgcggtgcg agcgagcacg cgatcccagc
     8221 cgcaccacgc agcgcacctt cgacggcagc ggcgacggtg cccgagtaga agacgtcgct
     8281 gccgaggttg tagccgtcgt tgatgcccga cacgacgacc gcaggtttgc gatcgcaaag
     8341 cttcaacact ccgaggtaca cgcagtcgac cggcgtgccc gacagcgaat agcggcgcgg
     8401 cttgacctcg gtgagccgca gcggcttgtg cagcgtgatc gcatgcgagg ccgcggaccg
     8461 ctggcgctcg ggcgccacca cgatcacgtc cgcgaactcc tcgatgcgct gcgcgagcgc
     8521 ttcgaggtgc ggcgagtgaa cgccgtcgtc gttggaacag agcacgagtg gacgcggcat
     8581 gccggacagt gaaagtgccg cggagatcgc ggcgggaaaa ggtggacggc tgggggagcc
     8641 gtccgtcccg gtcgaggcga caggatttga acctgcgacc cccagacccc cagtctggtg
     8701 cgctaccagg ctgcgctacg cctcgggtgg gaagcagttc ttagtagaac gacgccgctc
     8761 cgtcaacggc atcgactact tcactagtta cggcgcgctc gatcagacgg gcggcaagcc
     8821 gttgagaatg tttttcaaaa cctgcgaggg tttgaacgtc agcacgcggc gagccgggat
     8881 caggatcggc acggcgcgct gcgggtcgcg ccccatccgg tccttcttgt cgcgcacgac
     8941 gaagttaccg aaacccgaga tcttgatctt ctcgcctcgc tcgagcgtct ccttgacgac
     9001 gttgaagacg gtctcgacga tttccgcggc ctctttcttc gagaaaccgc ctactttttc
     9061 gtagaccgac tcgatgatgt ccgccttggt cattcgcgcc atagggtcac agtaacacac
     9121 tcccggcgcg aaaagccaat caagccaggt gcttcagcat ctgcatgccc ccacagagcg
     9181 agcaaaacga ggtgaacgcc tcgaagacgg cgtcgggagg ggcgtccggg aagcggaatc
     9241 gggccatcac gctgggcggg tcaaaacact cggtcgcccc gcacttgtgg cacggcgaga
     9301 tcgcccaatc ctcgcgctcg gccaggtact cgtccgtcac gtgcagcggg tgcggcatgc
     9361 catggaccga gatcaccttg agccgatcgc ccttcttcac cgactggagc gtgtggggct
     9421 ggttcagcag ctcaccgata tacacgtggc ggctcggctc gatggtgctt ggtggccggc
     9481 ccgcggtctc gggggcctcg atcgcgcttg gatgcagcag ctgacgcgtt ggtgcgcgcg
     9541 ccacgcgctg gattcgcacc cagcacagct cgggaccgcg gcgtgtgcgg cgcggatcgc
     9601 cgtcgtgcac gagcacctga gcgtcgtcgg gaaattgcgg atgaaacttc ccctcgagca
     9661 gaggatccgt tcgccacggc gcctcggcgt ccggctgcgg tccatagtga tcgagccacg
     9721 gcggtcgcgc cggcagcttg cgctcggcga cctcgtgcgc accgagctcg cggacttcga
     9781 tgcccgcgag ccgcaccgtg cgggatggca tcgtcgcaac gttcggatcg ctcgcgagtc
     9841 cgcccttcgc actcggcccg gcgtgctggt cgagatagac gaacgcgagg atcggttcgt
     9901 ccggcaaacg gtcgatccac acccagcgac cgaacggaac gctcacgcgg ccatcttacc
     9961 gagagtcgcg ctcggaaaaa tagtccgact attcttggcg ggccctaccg gcgctgggcc
    10021 ggcaggttct cgacgagccg gccgacgatc gtctcgtgcg ccttgtcgac ctcggcatcg
    10081 gtcagcgtgc gctcgggcga ccggtacgcg atcgaccaca gcatgctctt gtgtcccgcc
    10141 ggcagcttgg cgtcgcggta gtcctcgagc aggcgcacgc gcgacacgag cggttcgttc
    10201 gcggcctcga tgacatcgac gacgcgtgcc gccaagatgt cctccgcgag cagcagcgac
    10261 acgtcacgct cggagccggg gaagcgcgga atcgtccgca tctgcgccgg ctgcgcgagc
    10321 ggcagcttgt cgagctcgac ctcgaacgcg aatgcgggcc cggtgacacc gagcttctcg
    10381 cgcgtatcgg ggtgcacctc gccgaaccag ccgacgacca gcttgccgtc gtgcagctct
    10441 gcgaccagct cgccggcgac gccgggatgc aggtacgaga cgttcgagac cggattcgca
    10501 cgcacgcgca cctcgccggc gaccgcgcgg atcgcctcga gcgcgagcgc cttggcatcg
    10561 aacgcgtccc acggcgcgcc gtcgccgatc tggcccggcc gattgcccgc gagcacgccc
    10621 gcagcccagg tgggctcgtc ggccagctcg tgcatcgcgc cttcggtgac gcgctcaccg
    10681 cgccgcagga acacgctacc gacctcgaac agcgccacgt cgccacgtcc gaagctctgg
    10741 ttgcgcgcga tcgcggcgat caggttcggc agcagcgacg tgcgcatcgc ggcctggtcg
    10801 atgctcatcg ggttgcgaat gatgatcggc tgcgcacggc gatccgtcgc cgccaggccg
    10861 agcgcgtcga cgcgcgcgac cgactggaag ccgtacgtga tcgcttcggc ggcgccggct
    10921 gcagcaagcg cgcgccgtgc cacgtctgcg cgattcgctt cacgcgtctg cggcgcctgg
    10981 cgcatcaccg gcagcgtcga cggcacctgc tcgtagccgg tgacgcgcag gatctcttcg
    11041 atcacgtcga cctcgcgcac gacgtcgctg cgcggcgacg gtggcgtcac cgtgagctcg
    11101 ctctgtgtcg attgcacggt gaacccgagg cgctcgagcg catcgcgaca cgcggtctcg
    11161 gtgagcggca cgccggtgag catctgcacg cgtggcaggc gcgtcgggat aggtgcaatc
    11221 tcgcgttttc ccggatacgc gtcgaccgcg tcgccgatga ctttgccgcc accgaccatg
    11281 cagagcaggc gcgtcgcacg tgcggacgcg aagccggcga gctcgggatc gacgccgcgc
    11341 tcgaagcgat gcgacgcttc cgagtgcagg ccaagccggc gcgcggtttt gcgcaccgac
    11401 agcgggcgga agctcgccga ctcgagcagc acgcgcgtcg tgccgtcggt gacttcggta
    11461 tcggcaccgc ccatcacgcc ggcgagcgcg atgccgcgat caccgtcgcg gatcatcagg
    11521 tcccctttga cgagcgtgcg ctgctggtta tcgagcgtgg tgaacttctc gccatcggcc
    11581 gccggcgaca cgccgacgat gccggtcgtc agcttcgcgg catcgaacgc gtgcagcggc
    11641 tggccgagct cgaacatgat gtagttcgtg acgtcgacga ggttcgagat cgggcgcacg
    11701 ccgacggcgc ggagccgttg cgcgatccgc cgcggcgacg gcttcacggt caggccatcg
    11761 atcacgcgcg cgacgtagcg cgggcagtct tcgccgctcg cgatcgcgat ttcgaccttc
    11821 gcgttgccac cggccgcata cgccgacagg tccgcgtcgg gcagcacgag cttgccgcgc
    11881 agcatcgcga cgagctctcg cgcgacgccg atgtggccgt tcaagtcgcc gcggttcgcg
    11941 ggcgcattga cctcgagcag ccagtcgtcg atgccgagcg cgcgctgcgc cggtgccccg
    12001 agcggcgtcg cgtcgtcggc gtcgagcacg acgatgccgg cgtgatcttc ggacaagccg
    12061 agctcgtcct cggcgcacag catgccgggc gactcgatgc ccttgactgc cttcgcggcg
    12121 agcttgaggc cgccgggcag ctcggcgccg atctgcgccc acagaacctt gcgtcccggt
    12181 gccggcacgt tcggcgcgcc gcacacgacc tgggtcgccg cgccgccgcg ctccgtgatc
    12241 acgtcgacga gcgtcagctt gtcagcttgc ggatgcggcc gcttgccgac gacttcggcg
    12301 acgacgacgc ctttgaatcc ggcgccgagg ttcgtcatgc cttcgatctc gatgccgccg
    12361 cgcgtgagcg cacgcgcgcc ctcctccacc gtcggcacgt tgtcgaggtc gcacagctcg
    12421 agcagccagc tccacaggac cttcatcaga actgctccag gaagcgaatg tcgccttcgt
    12481 agtagtactt gatgtcgttg acgccgtggc gaagcatcgc catgcgctcg aggcccatgc
    12541 cgaacgcgaa gccggtgtag cgctcgccgt cgatgccgca gttcttgaac acgtcgggat
    12601 cgaccatgcc agcgccaccg atctcgaccc agccggtgtg cttgcagatg cggcatccgc
    12661 tgccaccaca gaagctgcac tgcatgtcga cttcagcgcc cggctcgacg aacgggaagt
    12721 agctcggacg caggcgaatg ccgaggtcct tcatgaagaa gcgcttcacg aagtgcagca
    12781 gcgtgccctt gaggtcggcg aacgagatgt tctcgtcgac ggcgaggcct tcgatctgcg
    12841 tgaacatcgg gctgtgcgtc ggatcgtcgt cgcggcgata gacgtgaccg ggcgcgacga
    12901 tgcgaatcgg cggcggctgc gtcagcatcg tgcggacctg caccgggctc gtgtgcgtgc
    12961 gcagcacgat ctcttccgac acgtagaacg tgtcctgcat gtcgcgcgcg gggtgatcct
    13021 tcggaatcgc gagcgcctcg aacgtgtgcc agtcggtctc gatctgcggg ccctcggcga
    13081 ccacgaagcc gagctcggcg aacacgctga tcgcttcgag ccgcacctgc gagagcagat
    13141 ggaggtgacc accgccgacc ggccgcgccg gcagcgtgat gtcaaccttg cgcgccaggt
    13201 cggcctgctt ctttgcgttg gtgagccgcg agaggctcgc gtcgacgatc tgctcgatct
    13261 tctctttgac cttgttcgca gcggcaccga cctgcggccg gtcgccagcg ggcagcttgc
    13321 cgagctcttt catcagctcg gagaccttgc ccttcttgcc gaggaactgc gcctgcgcgg
    13381 cgcggatcga ctgctcgtcg gcgagcgcct gcgcttgcgc gaggaactcg tcgccgagct
    13441 gctcgagctt cgcgattaga tctgttgtgg tgctcataaa acaaaaccgg cgaccctttt
    13501 gagccgccgg ttaaagtacc tggtgaagtc ggctctacgc gttgaggatc ttttcaaacg
    13561 caaccgggtc gctgatcgcg atttcggcga gcatcttgcg gttgagctgg acgttgccgc
    13621 cgctcagctt gcccatcagc ttcgagtagc tgatgccgag attgcgcgca gccgcgttga
    13681 tgcgctggat ccacagcgcg cggaagtcgc gcttcttcac cttgcgcgag cggtacatgt
    13741 gcatccacgc gtgctgaacg ctctcgttcg cgatcgcatg gcagttcttc cgcttgccgc
    13801 ggaaaccctt cgcccacttc aggacgcggt tacggcgacg gcgggctttg aatcctcgtt
    13861 tagcgcgggg catgacagtc ctctacaact aggcgtacgg cagtagtacg tcgatctggt
    13921 ggtgatgcgc ggacggcacg tagctcgcgt tgcggagctg acgcttacgc ttcttgctcc
    13981 gaccggtgag caggtggcgc ttgaacacgc gcataaactt gaacttgccc gacgcggtct
    14041 ttttgaaccg cttcttggcg ccggaatggg tcttgacctt gggcatgacg cgaggttgcg
    14101 ttttatgccc cagccgccgt ttggagacaa gcgatttgta cggattagtc gcgatcggtc
    14161 ggtttccggc cggggcccaa tatccgtggc atcccaggga tttcccgggg cttcgaccca
    14221 ggtggatcgg ccggttgcgc cgcggaggcc ccgtggcgcg acctaaatag cgccagaacc
    14281 cggcgcggca ggaggcgtag cctccggtcg ttcctcatcc catagacgct acgccaacgc
    14341 cggtttggcc cggcgcggcg ttcacgcgaa gctcacgcgc gcgcacgcga agcgcgtccg
    14401 tgaccgccgc gagcgcctac tcctcgtcgt cgtcgtcctc gacgtcggcg ttgatctcgt
    14461 cttcgtcgag atcctcatcc tcgtcgggct ccggcggcgg cgctgcgctc ggcttaccag
    14521 gctgctggtt gcgcttggcg gcttccgcgg ccatctgcgc cgcgacctgc gccttcttcg
    14581 cttcctgcgc cttgcgcacg acgcccggct tcggcgcgat gaccatgatc atgcgccgac
    14641 cttccatgtt cggcgtcgct tcgacgaccg caatgtcgtt gcaaaggtcg acgatcttgt
    14701 tcagcacgtt catgccggtc tgcgggtgcg tgatctcacg gccgcggaac acgacagcga
    14761 ggcggacctt gttgccaccc tcgaggaagc gacggacatg cttcaccttg aaggccatgt
    14821 cgtgctcttc cgtcttcggg cggaacttga tctccttcgt ctccaccgtc gacgcgcgct
    14881 tgcgcgcctg ctgtttcttc ttcgcctctt cgtacttgta cttgccgtaa tccatgatcc
    14941 ggcacaccgg cgggaacgcg cgtggagaaa tctcgacgag gtcgagtcct ttctcctcgg
    15001 cgagacgcaa cgcttcatgc gtcggcatca cgccaagctg ttcctcatcg gcgataacgc
    15061 ggacttcggg aacgcgaatc cggtgattga tacgaaggcc ctgctccggg cggcggcgat
    15121 cgaaacgtcc tccaggcggt cgaacgttca ttggaaaagg tcactctgct gcatgttttc
    15181 gtcggatctc ctgcgtttgt gcaggccgtg tttgtacccc gcccgccagt cggctgcgag
    15241 cgacttttcg tagcttcttt caaccccgcg acccggaggg gcgatccgcc ccacccggtc
    15301 agggtcgacc tagacctcgg cgctgccgcc ctgccagtcg agccgcagct tcgggccgtc
    15361 caccgtcgag aagcgaattc cgagtacggc ggactcggta cgagtagagc cataggcatc
    15421 gtcgagtgaa aggacctcga tggtcctggt cttcggttcg atgagccaca cctcgcccac
    15481 gccgcacatc gcgtagaacg ggagcttgtc gcggctctcg tcgttgggag agagaagctc
    15541 aaccacgatc tctgcgtgct gctcgagccc gcgttccgaa aagtcacttg gcgccacgac
    15601 cgaaagatcg ggaactcggt agttcttctc gccggctgcg ccaaataggc tggtctcgac
    15661 gaacaatcga tacccccgag cctcgacgag agccttcaac gcgtagatca actcgacttc
    15721 aagtccctga tgcgtggtcc ccggttgcgg aaccatgtgc agaactccgt cccagacctc
    15781 gtcacgtttg tcgtggccga gacggcggcg ctcctctagc cagtgcggag gcatttccag
    15841 cacgacggcg cgcatcgatc cgattctagc aggtgaggtc atgcacgcgt gttctgcagc
    15901 cagcgcgcca ccaccaacgt cgcagaatcg ctgcgtcgcg tttgtccgcc cggacgatcg
    15961 ccgcccgctc gtcggacggc gtgtccgtcg tgcggacgtg gcgaccgatt cgtcgccgaa
    16021 gtttcgtagg cgtgtgcggg attgaaccgc agacccccac cgtgtcaagg tggtgctctc
    16081 ccactgagct acacgcctgg gagggctgtt tacctacgcg atcgcgacta cctagtcaag
    16141 cagcgacacg gagtcgaacg cgggcgagcg cttggcgacg acgctgcttc ggtatccgaa
    16201 tcggtttcgg cggcgcgtag actgatcgcg tgcaggtcga acgctggtcg ctcccgcgcg
    16261 tggtcgcgtt tcgcttcggc gtcctcgcag cgctgctgct ggtgtcgccg accgtgcttt
    16321 ggccggtgcc gggcgccgca ccgctcgtga tcgcgatcat gcgtggctgg cactggctcg
    16381 cgactcagtt cggcgcgctg ctcgggctcg atgtcccgcc gctcgcaccg acgggtagcg
    16441 gcgacgcatt gtggaactgg ctgcaggccc tgctctcgat cctgctcgcc gtgcccggca
    16501 cgctcgtgtg gctacttgtc gatcgtcggc gcgcgcatcc gcgcctcgcc gccgcgatga
    16561 ccgtcttctt gcgctactac ctcgcggccg tgctgatcgg ctacggaatg gcgaaggtgg
    16621 tgccgatgca gttcgcaccg ctgccgctcg ctcgctacga cacgacgctc ggcgagatgt
    16681 cgccgatggg cctgatgtgg acgttcatgg cgttctcgca accgtacgtg ttcttcgccg
    16741 gcatcgcgga agtgatcggc agcgtgctgt tgctatcgcg ccgcacgtat gtcgccggtg
    16801 cgctgctgct catcgtcgtg atggcgaacg tcgtcgtgat gaacttctgc tacgacgttc
    16861 cggtcaagct gttctcgatg ctcctgatcg tgctgctgtg cgcgctcgtc gcgccgcatg
    16921 cgggacggct catcggtgca ttgctcggct atccgacgcg cgaagttccg ccgcgcgtgc
    16981 gtggctcggt gatgaccgag cgcgtgcggc tcgcgctcaa gctggcgatg gtcgcggtga
    17041 tcgcggtgcg cgcctacgag cacgtcgtga tctcgcgctg ggtcgaggag cagaagacga
    17101 tcacagcgct acacggcacg tggcgcgccg aacgtgtcgt gatcgatggc atcgagcggc
    17161 cgccgctatt cactgacgat gcgcgctggc gaaagctcat ctttcacgag tggggcctca
    17221 ccgtgcggtt cgccaccgat cgccgcctca acttcgcgac gcaggtcgac gcgacggcgc
    17281 agacgatcaa cgtcggacaa ggtccgtttc ggcaggtgtg gcgctaccgg ctcgtcgata
    17341 gcgagcacct gatcatcgac acaccgacgg tccgcggcga gctcgtgctc gagcccgagc
    17401 caccgctcac gacgcgc
//
LOCUS       DASUFX010000154         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_718534,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000154 DASUFX010000000
VERSION     DASUFX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718534"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2010
                     /locus_tag="VFV99_06125"
     CDS             109..2010
                     /locus_tag="VFV99_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5058915.1"
                     /translation="MLALAAGCSSSTSSSNPPADGDMPGDSTGSGSDGAGSGSDDGGT
                     TGPVEPPPATDPVEPVWEPNSAMTRIKSPNQRMHFTAGLPFRILADGNDPLAYQCPPG
                     HPPYACSDSTMTFYVDGTSVGVIPPDPANQNLWELRLPNGLPAGNHVLTVKFKPHGVA
                     AVDGIVPIYITVDPAPPKQVVTLTQDMVLTGSTNLDWTNVFVKGNGHTVTAAPGYSGK
                     IIIRDSFVTGLAAFDNQIGMRVTTTGGVEIRKSIFEATAPLQLTVNGSAPVTINNNEL
                     RSTNYVTYVSSDPDKSPILELSGNTSGAKTMQGNNIGAGIIRIENMAGWQIGGLRDSQ
                     SNIFVGPRVVLTLVDSPNAVIQGNYMHHDYYNGFSQGYNLRLDGGSSALAEHNVIRDS
                     SWPLQSFGGELRYNLLINSGHDFVRSLQSGARLHHNIFAHQQAPNSGYEGGVLMYGGE
                     QNVMFDNNTFDVGGAAARFDGPALSIGSGVQLASLRNNAFTQWSSISWGNRSIVAGAK
                     GESSVGGSRITRADYNAWYNPLATNTGHYMPGIAGSAGTHDVSANPLFAGPVPQVPCK
                     IDEGSMWSRTYGVSQLLQYYRSLYTPGAGSPLIDAGDPSDGAGNDIGAIGAGAPNAAD
                     KFGTVMQAN"
     gene            complement(2011..3186)
                     /locus_tag="VFV99_06130"
     CDS             complement(2011..3186)
                     /locus_tag="VFV99_06130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5058916.1"
                     /translation="MQLYDLIIIGGGPAGLSTALHVHALAPHLKLVVLEAKSYPREKI
                     CAGGIGARAFAILDKLGITVDVPMVKLDAVAMRIGGEEVVARVPGCGVVVRRVEFDHA
                     LAKQTLARGIDVRDATPVSSIEVTDEHVQVVTERGDLLRARAIVGADGVSGITRRATG
                     FPRCELRAQVCELDTEATSFDMPRDMVVFDFEARDLNGYRWDFPTIVGGKDMVCRGAY
                     VIHNMGHDSPKTRMATYLRERGLDLANYKLKQYAERGFEPGAEIASPRVLLVGEAAGI
                     DIATGEGIAQAIEYGALAGTYLASAFERDNLLFSTWRHTVERRHIGKQLRIRHLCYRF
                     FYGRHRPRMERMLPKLEKWFRVGVQDFAGVPLSKLALAQGAAIFIGQLAREGMRAPD"
     gene            complement(3216..3944)
                     /locus_tag="VFV99_06135"
     CDS             complement(3216..3944)
                     /locus_tag="VFV99_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058917.1"
                     /translation="MGYVSRHKRRDLSKYQTIYEDPEYDRLAASLTSRDDKVTAGMLA
                     GGSTLETMGGLAAVILSVIGFEERANEMGSIATIAIGIALLSHGASLMARWGHAFRRV
                     RGARPDRDEMVESIGTEVFGGVVGIVLGVIALVGIKPAVILPVAAIVYGSSLLLGGAS
                     QPDLVYLAPETNPRYARVTFSAIQASGGIMVLVGVAAAVLGVLALLEVGPPISLALVA
                     MLSIGFSLLFAGGALTARFVRRFT"
     gene            4023..4274
                     /locus_tag="VFV99_06140"
     CDS             4023..4274
                     /locus_tag="VFV99_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058918.1"
                     /translation="MHADRELLAMLSVGGSRERPIKVASASVVEVRTSALPCPLCGGS
                     YRLDEHVSLAPGLRRVDVHCRHCSTPRSLWFRIVVDDLN"
     gene            complement(4276..4596)
                     /locus_tag="VFV99_06145"
     CDS             complement(4276..4596)
                     /locus_tag="VFV99_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEX5058919.1"
                     /translation="MPPQGRLGDLSNVPADAHGCIACPHPCVGPAIAGSPNVNVNKMP
                     ALRVDDTGIHMACCGSNTWTAQAGSSTVFINNKAAHRQGDADKHCGGMGQLISGSPNV
                     IVGG"
     gene            complement(4596..>4786)
                     /locus_tag="VFV99_06150"
     CDS             complement(4596..>4786)
                     /locus_tag="VFV99_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058920.1"
                     /translation="PFHAELFEAIIDGFMKCLTIWKASTMVTNVLGTGPVPTFAPPYV
                     PVGPVVGGVGTMTPGGFV"
BASE COUNT          839 a         1579 c         1568 g          800 t
ORIGIN      
        1 gtgctgctcg tcgggtgggc atcgcatgtg aacgatgcga gataggtcgc gatgtactcg
       61 acctctgttg ttcacggcgc gcgcgagagc gcacgcttgg tcacaatcgt gctggctctt
      121 gctgccggct gttcgagctc gacaagctcg tcgaacccac ccgctgacgg cgacatgccg
      181 ggcgattcca ccggttcagg ttcagacggt gcgggctcgg gcagcgacga cggcgggacg
      241 acgggacccg tcgagccgcc tcccgctacc gatccggtcg agcccgtgtg ggagccgaac
      301 tcggccatga cgcggatcaa gtcaccgaat caacgcatgc atttcacggc aggcctgccg
      361 ttccgcattc tcgccgacgg taacgatccg ctcgcgtatc agtgcccgcc cgggcacccg
      421 ccgtacgcgt gctccgacag cacgatgacg ttctacgtcg acggcacctc cgtcggtgtg
      481 attccgcccg atccggcgaa ccagaacctc tgggagcttc gcctgccgaa tggtttgccg
      541 gccggcaatc acgtgctgac cgtgaagttc aagccgcatg gcgtggccgc ggtcgatggg
      601 atcgtgccga tctacatcac ggtcgatccg gcgccgccga agcaggtcgt caccttgacg
      661 caagacatgg tgctcaccgg ttccaccaat ctcgactgga cgaacgtgtt cgtgaagggc
      721 aatggccaca cggtcacggc cgcgcccggg tattccggca agatcatcat tcgcgacagt
      781 ttcgtgacgg gactcgctgc gttcgataac cagatcggga tgcgcgtgac gaccaccggt
      841 ggcgtcgaaa tcaggaagtc gatcttcgag gcgacagcgc cgcttcagct caccgtgaac
      901 ggcagcgcgc cggtcacgat caacaacaac gagcttcgct cgacgaacta cgtcacgtac
      961 gtgtcgtcgg atccggacaa atcgccgatc ctcgagctgt cagggaacac gtcgggcgcg
     1021 aagacgatgc aaggtaacaa catcggggcc ggcatcatcc gcatcgagaa catggcgggt
     1081 tggcagatcg gcggcctgcg tgactcgcag agcaacatct tcgtcgggcc gcgcgtcgta
     1141 ctcacgctgg tcgactcgcc gaacgcggtc atccagggca actacatgca tcacgattac
     1201 tacaacgggt tctcgcaggg ctacaacctc cggctcgacg gtggcagcag cgcactcgcg
     1261 gagcacaacg tgattcgcga ctcgtcgtgg ccgttgcagt cattcggcgg cgagctccgc
     1321 tacaacctgc tgatcaacag tggtcacgat ttcgtgcgct cgctccagag cggtgcgcgg
     1381 ctccaccaca acatcttcgc gcaccagcag gcgccgaact cgggctacga gggtggcgtc
     1441 ctcatgtacg gcggcgagca gaacgtgatg ttcgacaaca acacgttcga tgtcggtgga
     1501 gccgccgcgc ggttcgacgg gccggcactc tcgatcgggt cgggtgtgca actcgcctcg
     1561 ctgcgcaaca acgcgttcac gcagtggtcg agcatcagct ggggcaaccg ctcgatcgtc
     1621 gccggcgcca agggtgaatc gagcgtcggc ggctcccgga tcaccagggc cgactacaac
     1681 gcctggtaca acccgctcgc gacgaacacg ggccactaca tgcccggcat cgccggtagc
     1741 gcgggcaccc acgacgtctc ggcgaacccg ctgttcgccg gtccggttcc acaggtgccc
     1801 tgcaagatcg acgaaggctc gatgtggagc cgcacgtacg gcgtctcgca gctgctccag
     1861 tactaccgct cgctctacac cccaggcgcg ggctcgccgc tcatcgacgc gggcgatcca
     1921 agcgacggcg cgggcaacga catcggcgcc atcggcgcag gcgctccgaa cgcagccgac
     1981 aagttcggca ccgtgatgca ggcgaactaa tcagtccggt gcgcgcatgc cttcgcgggc
     2041 gagctggccg atgaagatcg cggcgccctg tgcgagggcg agcttcgaca gcggcacgcc
     2101 cgcgaagtcc tggacgccga cgcgaaacca cttctcgagc ttcggcagca tgcgctccat
     2161 ccgcggccgg tgcctaccgt agaagaagcg gtagcagagg tggcgaatgc ggagctgctt
     2221 gccgatgtgg cggcgctcga cagtgtgccg ccacgtcgag aacagcaagt tgtcacgctc
     2281 gaaggcgctg gcgagatagg tgccggccag cgcgccgtat tcgatcgctt gcgcgatgcc
     2341 ttcgccggtc gcgatgtcga tgccagccgc ttcaccgacg agcagcacac gtgggcttgc
     2401 gatctcggca ccaggctcga agccgcgctc cgcgtactgc ttgagcttgt agttcgcgag
     2461 gtcgagtccg cgctcgcgca gataggttgc cattcgcgtc ttcggcgagt cgtggcccat
     2521 gttgtggatc acgtacgcgc cgcgacacac catgtccttg ccgccgacga tggtgggaaa
     2581 gtcccatctg tagccgttga ggtcgcgcgc ctcgaagtcg aacacgacca tgtcgcgcgg
     2641 catgtcgaac gacgtcgcct cggtgtcgag ctcgcacacc tgtgcgcgaa gctcgcagcg
     2701 cggaaagccg gtcgcgcgcc gcgtgatgcc actgacgcca tcggcgccga cgatcgcgcg
     2761 cgcgcggagt aggtcgccgc gctcggtcac gacttggacg tgttcgtcgg tgacttcgat
     2821 cgagctcacc ggggtcgcgt cgcgcacgtc gatgccacgc gccagcgttt gcttggcgag
     2881 cgcgtgatcg aactcgacgc ggcggacgac cacaccgcag cccggcacgc gcgctacgac
     2941 ttcctcgcca ccgatgcgca tcgcgaccgc gtcgagcttc accatcggta catcgacggt
     3001 gatgccgagc ttgtcgagga tcgcgaacgc gcgcgcgccg atgccgccgg cgcagatctt
     3061 ctcgcgcgga taggacttcg cttcgagcac gaccagcttc agatgtggcg cgagcgcgtg
     3121 gacgtgcagt gccgtcgaca ggccggcggg accgccgccg atgatgatga ggtcgtagag
     3181 ctgcagggcg ccgggaaggt acctccgccg ggccgttacg tgaagcgccg gacaaaccgc
     3241 gccgtcagcg ctccgcccgc aaacagcaac gaaaacccga tgctgagcat cgcaacgagc
     3301 gcgagcgaga tcggcggtcc gacctcgagg agcgcgagca cgccgagcac cgctgcggcg
     3361 acaccgacga gcaccatgat gccgcccgat gcctggattg ccgagaacgt gacgcgcgcg
     3421 taccgcgggt tcgtttcggg tgcgaggtac acgagatcgg gctgggatgc gccaccgagc
     3481 aagagcgagc tgccgtagac gatcgcggcg accggcagga tcacggccgg tttgatgccg
     3541 acgagcgcga tcacaccgag cacgatgccg accacgccgc cgaacacttc ggtgccgatg
     3601 ctctcgacca tctcgtcgcg atcgggccgt gcaccgcgga ccctgcgaaa cgcgtggccc
     3661 cagcgcgcca tcagcgacgc gccgtgggag agcagtgcga tgccgatcgc gatcgtcgcg
     3721 atcgagccca tctcgttcgc gcgctcctcg aaaccgatga cgctgaggat gacggccgcg
     3781 agaccgccca tcgtctcgag ggtgctgccg ccggcgagca tgccggccgt gaccttgtcg
     3841 tcgcgtgagg tcagactagc cgccagtcga tcgtattcgg gatcttcgta gatcgtttgg
     3901 tacttggata ggtcgcggcg cttgtgacgg ctgacgtaac ccatgcacgg tatggctgca
     3961 tcgctcatgc ctgcctgatc gcgtaggtgc agccgaaact cgcgaacgtt atcttcttag
     4021 ctatgcacgc tgatcgagag ctacttgcca tgctgtcggt gggcggctca cgcgagcggc
     4081 cgatcaaagt tgcgtcagcc tccgtcgtcg aggttcgtac gagcgcgctg ccgtgcccgc
     4141 tgtgcggcgg cagctatcga ctcgacgagc acgtgtcgct cgcgccgggg ctgcggcgcg
     4201 tcgacgtgca ctgccggcac tgctcgacgc cgcgttcgct gtggttccgg atcgtcgtcg
     4261 acgatctgaa ctgaactagc cgccgacgat cacgttcggg gagcccgaga tgagctggcc
     4321 catgccgccg cagtgcttgt cggcgtcgcc ttggcggtgc gccgctttgt tgttgatgaa
     4381 caccgtgctc gagccggcct gcgcggtcca cgtgttcgag ccgcagcacg ccatgtgaat
     4441 gcctgtatcg tcgacacgca acgcgggcat cttgttgacg ttcacgttcg gcgagcccgc
     4501 gatcgcggga ccgacacacg gatgcggaca cgcgatgcag ccatgcgcat cggccgggac
     4561 gttcgagaga tcgccgagtc gtccttgagg tggcattaca cgaaccctcc tggcgtcatc
     4621 gtgccgacgc caccgacgac cggaccgacg ggcacgtagg gcggcgcgaa cgtcggcacc
     4681 ggacccgtgc cgagcacgtt cgtgaccatc gtcgacgctt tccagatcgt gaggcacttc
     4741 atgaagccgt cgatgatcgc ttcgaacagc tcggcgtgaa acggtg
//
LOCUS       DASUFX010000155        12075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_718606,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000155 DASUFX010000000
VERSION     DASUFX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718606"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFV99_06155"
     CDS             <1..414
                     /locus_tag="VFV99_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058921.1"
                     /translation="TNDVYVTGTRGTILHWTGSQMVDESIPPSVTVTSIWGVNGNDLW
                     AVAQTGLIYRKMNNGIWIQQTSPTTQFLWQIWGTAADDIWAVGDGATVIHYDGTQWST
                     VSVPIASTVSLRGIAPVPGGGLRAVGTSGTVLAHP"
     gene            complement(411..1079)
                     /locus_tag="VFV99_06160"
     CDS             complement(411..1079)
                     /locus_tag="VFV99_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5058922.1"
                     /translation="MWWSYYAFWLLVPTVLAVVSAHPVVLVVVVVGLVARRWLPDPFM
                     YLRHAGRVHSLRQQVEINQANATARAQLAEIWLAKRRPRRAIPLLQQALERDAGSAEL
                     RYLLGVAYLRAGQPQKALEPLSEALAKDQKLRYGSAYLAIGDALLAVGRKDEAIEAYE
                     RYVKINTSSLEGYVKLSDARHKNGDKAGYEKARAEVFETYRVLPPYQRRRQLGWWLRA
                     KFLS"
     gene            1186..1401
                     /locus_tag="VFV99_06165"
     CDS             1186..1401
                     /locus_tag="VFV99_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058923.1"
                     /translation="MAQLWSPLKAAIVVFLVVVVIGAVVGVVITSANGTIHDHSFDRG
                     QAMGQGIGTIAIVAGVVAYFIQRKRRG"
     gene            complement(1398..2135)
                     /locus_tag="VFV99_06170"
     CDS             complement(1398..2135)
                     /locus_tag="VFV99_06170"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5058924.1"
                     /translation="MTFLESVGAAVRSQRERKGWSRRELAGASGVSERFLAQLETGDG
                     NISLRRFSEVAHALGTTPAALLAPAEAPAAHKPIALLGVRGAGKSTVGAALARKLGMR
                     FVEVDQEIEAAAGLALGDVFTLHGEAYYRRVEREVLTRLLADANPMVLATGGSIVNDP
                     TNYALLEKRAYTIWLRARPEDHWNRVVAQGDQRPMAENPHAFEELRALLAARQKLYAR
                     ADRTIDTSRRSIKQVVAEIADATEGSS"
     gene            2208..3842
                     /gene="boxC"
                     /locus_tag="VFV99_06175"
     CDS             2208..3842
                     /gene="boxC"
                     /locus_tag="VFV99_06175"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX5058925.1"
                     /translation="MDPTSFETHPSRYKHWRLTVNGDIATMVMNVDNEHPHRPGYELK
                     LNSYDLSVDIELADAIQRLRFEHPEVKCLVFTADLDRVFCSGANIYMLGQSTHSFKVN
                     FCKFTNETRLYLEDASAHSGLASLAACKGTTAGGGYELALACDETMLVDDGSSAVSFP
                     ETPLLAVLPGTGGLTRLVDKRKVRRDRADVFCTTAEGIKGKRAKDWGLVDHLVSRTKW
                     DEAVATRAKELAAKQQVSRGPAVNLSPLEPKVTPERIAYRFVELTFDAAERTANLVVR
                     APEGAEDPDKLAAAGTEPWALRAFRELDDALLRLRFDYPEINVVTVRTTGKRDAVKEC
                     DELLAKSTSGFAREVRLLQRRVLKRYDNTARSLYAVADTADSCFAGVLLELALGADRF
                     YMLIDGDEKIGVETSVANTGFMTMSSGLSRLDARFYAEQGRAQKVLARGTEGPIPSEE
                     AEQLGIATIAADDIDFEDELRIAIEERASLSPDALTGMEASLRFVGPETLETKIFGRL
                     SAWQNWIFTRANSTGQHGALTLYGQPERPQFQWKRT"
     gene            3846..5255
                     /gene="boxB"
                     /locus_tag="VFV99_06180"
     CDS             3846..5255
                     /gene="boxB"
                     /locus_tag="VFV99_06180"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX5058926.1"
                     /translation="MSTVLTDEKIPNNVNLAGDKRLQRALEAWQPKFIDWWKDMGPVG
                     WQERDIYLRTAISVEANGWAHFDYVKMPDYRWGIFLEPKDPNRVHGFGDNMGQPVWDE
                     VPGEFRNMLRRLVVTQGDTEPASVEQQRNLGATAPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHTYFGRDGREEAEELLMRRSGDVDKPRILGAFNEPTSHWLSFFMFTMFTDRDGKY
                     QLAALAESGFDPLARTCRFMLTEEAHHMFVGETGVQRIVKRACELMKEPGGDVRALGG
                     VDLPTIQKYLNLWYSLSLDLFGGEVSSNAADAFAAGLKGRYKEDRYEEHKLIGSHFAM
                     DVLEDGKVVHKDIAMRNALNEVLRSEYIKDCQRGVDRWNKTIKDEGIDFELRLPSNKF
                     HRQIGMWAGHRFSPAGEMLDTATWDARRGEWLPNDADETYIKSLMKPVHERGKMANWI
                     APPAKGINGLPGDFEYVRL"
     gene            complement(5256..6983)
                     /locus_tag="VFV99_06185"
     CDS             complement(5256..6983)
                     /locus_tag="VFV99_06185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5058927.1"
                     /translation="MHRDDDTRDLSPTHVDDVRTTPVNVDDAPLVADADRYASVALLG
                     KGGMGEIRLCKDSRIARQVAVKMLRGSLRDDPEYRARFLREARLQGQLEHPAIVPVHD
                     LGTTGDGDLYFTMKRVRGATLQEALTAIEGGDRTRFSRRRLLTAFSSVCLAVDFAHSR
                     GVVHRDLKPSNVMLGDFGEVYVLDWGIAKVMDRADAPLAEGVEVPDTDTPTRVGAVMG
                     TPRYMAPEQRMGHADARSDVFSLGLILGEILACDRDAPPELVAIASHATQPEPEDRYA
                     SARELHDAIERFLDGDRDLEARRRAADEHAHQAEQALERAQVAPTPDARVEAARHVGR
                     ALGMEPGNVRAMRALTRLLHEVPNHLPPAAQAAMERRWLERSMRMAKSGILVMFAFFT
                     FIPFAAVLGVRHWGALGAFSGFIVGALLFHWLSRGQIYVWQRAMTYVMTLGALAIMSR
                     SMGLLGVLPVAMTLVLLAYRLYVVTWQHGLALSTGTFITLLLPFVLEWLHVIPPIHVF
                     RDGGVTIVPQLHALPPGLTQLYLIISCAFALGVTVIYARQYVVELRRAEARAAFHTWQ
                     LEQLLPPDA"
     gene            7157..7720
                     /locus_tag="VFV99_06190"
     CDS             7157..7720
                     /locus_tag="VFV99_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058928.1"
                     /translation="MRCVIIILVLLVATPRAFADEAKLAEKTRVPASYAGLGIEANDQ
                     RQGRLGFSGDAGLRLDNSPLFLRARLTFGLSGADGHYEQLRVGLEGRSCGRMWVCVFA
                     GLDFGGQRDHAVGAPFNFQGWLDNEPPPMTVVDSLDVLLVPRIGIETPTKFRARIALD
                     AVMFKELEGQGTTGVAWTPSISAAYTF"
     gene            complement(7717..8226)
                     /locus_tag="VFV99_06195"
     CDS             complement(7717..8226)
                     /locus_tag="VFV99_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058929.1"
                     /translation="MSSDPDPVADELLALLVKYADAPFGLLADDEVQRRFRIQSDRGI
                     KIRLSLEPDKYVVESPVIDCVSRLHLCRARCCSLDVVLSPQDLAERKLPVIVDQPYLL
                     ARDPITKKCACMDSKGACTAYDYRPATCRSYDCREDTRVWVDFEARIPAPLPENLKPL
                     ENAFVSDDD"
     gene            complement(8223..8801)
                     /locus_tag="VFV99_06200"
     CDS             complement(8223..8801)
                     /locus_tag="VFV99_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058930.1"
                     /translation="MSSDPDPVPDELLALMEKLASDLPQLPNHDEALRRFEWLLDALI
                     MRGQLPERFRRMAAKIQADRGTKIRLSLATDKYVVDSPDIDCASRIHLCGARCCSFEV
                     ALSPQDIVERKLPFLVEQPYLLPRDPITKKCACMDAKGACTAYDYRPATCRTYDCRED
                     ARVWIDYAARIPAPLPENLKPLENAFVNPEDE"
     gene            complement(8798..9835)
                     /gene="speB"
                     /locus_tag="VFV99_06205"
     CDS             complement(8798..9835)
                     /gene="speB"
                     /locus_tag="VFV99_06205"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX5058931.1"
                     /translation="MSTQEHDADAAPPADAGLFGLPTTVDEARLVIVPVPWDATTSYR
                     PGTSRGPAAIRSASHQLDLYDADLGEPWRAGITMLDIPDEVVRWNMSARVDAEKIIEV
                     GGRIGDDTALAQSLARVNEAGARLNRHVYEQVDRLLKAGKLVGVVGGDHSVPFGAIAR
                     MAESYDSFGILHVDAHCDLRDAYEGFEWSHASIMYNVIKKIPQVKRLVQVGIRDFAKR
                     EADAVAAANGRVVVHHDASLAERGFAGETWRDICDAIVKDLPRQVYVSFDIDGLDPAL
                     CPSTGTPVPGGLSFQQAAYLIKRVVERGHTIIGFDLNEVVPGPDGDEWDANVGARVLY
                     KLCGWTLHSNR"
     gene            complement(9832..10038)
                     /locus_tag="VFV99_06210"
     CDS             complement(9832..10038)
                     /locus_tag="VFV99_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058932.1"
                     /translation="MRIALVIVVSLATLAFGGCGKKKAPQSPAASESQQMKEGEDEKD
                     VKAPAEGDEDDRDTRSSDPQEGGE"
     gene            complement(10048..11199)
                     /gene="rpsA"
                     /locus_tag="VFV99_06215"
     CDS             complement(10048..11199)
                     /gene="rpsA"
                     /locus_tag="VFV99_06215"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5058933.1"
                     /translation="MATEDEDFAAMFAESERDKPKAKRPKIGDVIKGKVISIGKEAVF
                     VDLGGKAEGQLEKNQVSDKDGKLLVKVGDVIEARIVSDTGGVLMLRTKVTRGPEARAE
                     LQQAFELGIPVEGTVTEVIKGGVSVDVAGVRGFCPASQVDNRFVEDLATYVGQKLTFK
                     ITRYEPRNLVLSRRALVEEAQEKLATETRKKLAPGAVLRGKVVGFKPFGAFVDLGGIE
                     GMLHVSELGFARVEKPEDVLSLGQELDVAVLKIEPGEKGDRISLSLKALANDPWRDAT
                     ASLGEGARVKGTITRLQPFGAFVEIAPGVEGLVHISELGAGRRINHPKEVVSIGQQVE
                     ATVLAVDHDKRRLSLSLAASTDASPDDVAAVPRAPQKLGTLGDLLQRKK"
     gene            complement(11271..>12075)
                     /locus_tag="VFV99_06220"
     CDS             complement(11271..>12075)
                     /locus_tag="VFV99_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5058934.1"
                     /translation="ILGKGKLDTLLLDVTSLASIEAAARAVDALTSGRGLDVLVNNAG
                     FGVLGPTSEITDSEMRRQYETNVFGLMNVTRAFLPAMMQRRSGRIINVSSVGGRITLP
                     YFGVYNSTKYAVESLSDAMRYELRPFGIDVAMIEPGVIRTNFEATAAGNLKQLENTPY
                     KVAVAKYEQMSKTADRFASNPIVIAKAIARAVNARRPAARYVAPFSTNFVILFARMFP
                     TRVWDWAMRKVGFLSARSLGLTNAARTVIDDLVVPPPSSAKPAEVRASN"
BASE COUNT         2075 a         3950 c         3982 g         2068 t
ORIGIN      
        1 acgaacgatg tgtacgtcac cggcacgcgc ggaacgatcc tgcattggac cggcagccag
       61 atggtggacg agagcatccc gccgagtgtc accgtcacgt cgatctgggg tgtcaacgga
      121 aacgatctgt gggcggtggc gcagacgggc ctcatctacc gcaagatgaa taacggaatc
      181 tggatccagc agacgagccc gacgactcag ttcctgtggc agatctgggg aacggccgcg
      241 gacgatatct gggcagtcgg tgatggcgcg accgtcatcc actacgacgg cacgcaatgg
      301 tcgacggtgt cggtgccgat cgcgtcgacg gtctcgttgc gcggtatcgc gccggttcca
      361 ggcggcggcc tgcgtgcagt cggcacgtca ggcaccgtgc tcgcgcatcc ctagctgagg
      421 aacttcgcgc gcagccacca gccgagctgg cgacggcgtt gatacggcgg cagcacgcga
      481 tacgtctcga acacctcggc acgcgctttc tcgtagccgg ccttgtcgcc gttcttgtgc
      541 cgcgcgtccg atagcttgac gtagccttcg aggctcgacg tgttgatctt cacgtaacgc
      601 tcgtacgctt cgatcgcctc gtctttgcgg cccaccgcaa gcagcgcgtc accgattgcg
      661 aggtatgcgc tgccgtaacg gagcttctgg tctttcgcga gtgcctcgga caatggctcg
      721 agcgcctttt gcggctgccc cgcgcgcagg tacgccacgc cgagcaagta gcggagctcc
      781 gcagagccgg cgtcgcgttc gagtgcttgt tgaagcagcg ggatggcgcg gcgcggccgg
      841 cgcttcgcga gccagatctc ggccagctgt gcacgcgcgg tcgcgttcgc ctggttgatc
      901 tcgacctgct ggcgaaggct gtgcacgcgg ccagcgtggc gtagatacat gaatggatcg
      961 ggcagccaac gacgcgccac caacccgacg acgacgacca cgagcacgac ggggtgggcc
     1021 gacacgaccg ccagcacggt cggcacgagc agccagaatg cgtagtagga ccaccacata
     1081 cgagaaggag ccgagctccg agcccgggtc gcgcgatctt gccgatcttc gcgtcggaaa
     1141 caacgatttt atgcgaccga acgcgcgtga gaagctcgat ccggcatggc tcagctgtgg
     1201 tcacccctaa aggccgctat cgtggtgttt ctggtcgtgg tggtcatcgg ggccgttgtt
     1261 ggcgtcgtga tcacgagcgc gaacggcacg atccacgatc attcgttcga tcggggccag
     1321 gcgatgggcc agggcattgg cacgatcgcg atcgtcgcag gcgttgtggc ctacttcatc
     1381 cagcgcaagc gccgcggtta gctcgatccc tctgtcgcgt cggcgatctc ggcgacgacc
     1441 tgcttgatgc tgcgccggct cgtgtcgatc gtgcgatccg cgcgcgcata aagcttctga
     1501 cgagcggcga gcaacgcgcg cagctcttcg aacgcgtgtg gattctccgc catcggccgt
     1561 tggtcgcctt gggcgacgac gcggttccag tgatcctcgg ggcgtgcgcg cagccagatc
     1621 gtgtacgccc gcttctcgag cagcgcgtag ttcgtcgggt cgttgacgat cgagccgccg
     1681 gtcgcgagca ccatcggatt cgcatccgcg agcaggcggg tgagcacttc gcgttcgacg
     1741 cgccggtaat acgcttcgcc gtggagtgtg aatacgtcgc cgagcgcgag gccagcggcg
     1801 gcttcgatct cttgatcgac ctcgacgaac cgcataccga gcttgcgcgc gagcgccgcg
     1861 ccgacggtcg acttgccggc gccacgcaca ccgagcagtg cgatcggctt gtgagcggca
     1921 ggtgcttcgg ccggagcgag cagagcggcc ggcgtggtgc caagcgcatg cgcgacctcg
     1981 gagaaccggc gtagcgagat attgccgtcc ccggtctcga gctgggccaa gaaccgttcg
     2041 gagacgccac tcgcgcccgc gagctcgcgc cgcgaccacc ccttgcgctc gcgttgcgaa
     2101 cgcacggctg cgccgacgga ttcgaggaac gtcatggagg acgtagcgaa atatactgca
     2161 tgcagaaggt gcgtgacaag cagtaaattg cctgatagac gatcgggatg gatcccacga
     2221 gcttcgagac ccatccaagc cgctacaaac actggcggct cacggtgaac ggcgacatcg
     2281 ccaccatggt gatgaacgtc gacaacgagc acccgcatcg cccgggctac gagctcaagc
     2341 tgaactcgta cgacctcagc gtcgacatcg agctcgcgga cgccatccag cgcctccgct
     2401 tcgagcatcc cgaggtgaag tgcctcgtgt tcacggcgga cctcgaccgc gtgttctgct
     2461 cgggcgcgaa catctacatg ctcggccaat cgacgcactc gttcaaagtg aacttctgca
     2521 agttcacgaa cgagacccgg ctctatctcg aagacgcatc ggcgcactcg ggcctcgcgt
     2581 cgcttgccgc gtgcaagggc acgacggccg gtggtggtta cgagctcgcg ctcgcctgcg
     2641 acgagacgat gctcgtcgac gacggcagct cggcggtcag cttcccggag acgccgctcc
     2701 tcgccgtgct tcctggcacc ggcggtctca cccgcctcgt cgacaagcgc aaggtccgtc
     2761 gcgatcgcgc cgacgtgttc tgcacgacgg ccgaaggcat caagggcaag cgcgcgaagg
     2821 actggggcct tgtcgatcac ctcgtgtcgc gcacgaagtg ggacgaggca gtcgccacgc
     2881 gcgcgaagga gctcgctgcc aagcagcaag tatcgcgtgg cccagcggtc aacttgtcgc
     2941 cgctcgaacc aaaggtgaca ccggagcgca tcgcgtatcg cttcgtcgag ctcacgttcg
     3001 atgccgcgga gcgcaccgcg aacctcgtcg tgcgcgcacc cgaaggcgcc gaggatcccg
     3061 acaagctcgc ggctgccggc accgagccgt gggcactgcg cgcgttccgc gagctcgacg
     3121 acgcgctgct ccgcctgcgg ttcgactatc ccgagatcaa tgtcgtcacc gtccgcacga
     3181 ccggtaagcg cgatgcggta aaggagtgcg acgagctgct cgcgaagtcg acgtcggggt
     3241 tcgcgcgcga ggtgcggctg ctgcagcgcc gcgtgctcaa gcgctacgac aacacggcgc
     3301 gcagcctcta cgctgtggca gataccgccg acagctgctt tgcgggcgtg ctgctcgagc
     3361 tcgcgctcgg tgccgaccgc ttctacatgt tgatcgacgg cgacgagaag atcggcgtcg
     3421 agaccagcgt cgcgaacacc ggcttcatga cgatgtcgag cgggctgtcg cgcctcgacg
     3481 cgcggttcta cgccgagcaa ggtcgcgcac agaaggtgct cgcgcggggc accgagggcc
     3541 cgattccgag cgaggaggcc gagcagctcg gcatcgcgac gatcgccgcc gacgatatcg
     3601 acttcgagga cgagctccgc atcgcgatcg aagagcgcgc gtcgctgtcg cccgacgcgc
     3661 tcaccggcat ggaagcgtcg ctccgcttcg tcggtccgga gacactcgag accaagatct
     3721 tcggccggct ctcggcctgg cagaactgga tcttcacccg cgccaactcg accggccagc
     3781 acggcgcgct cacgctctac ggccaacccg aacgcccaca gttccagtgg aagaggacct
     3841 agccgatgtc caccgtactc accgacgaga agattccgaa caacgtcaac ctggcgggtg
     3901 acaagcgcct gcagcgcgcg ctcgaagcgt ggcagccgaa gttcatcgac tggtggaaag
     3961 acatgggccc ggtcggttgg caggagcgcg acatctacct gcgcaccgcg atctcggtcg
     4021 aggccaacgg ctgggcgcac ttcgactacg tgaagatgcc cgactaccgc tggggcattt
     4081 tcctcgagcc gaaggatccg aaccgcgtgc acggcttcgg cgacaacatg gggcagcccg
     4141 tgtgggacga agtgccgggc gagttccgca acatgttgcg tcgcctcgtc gtcacccaag
     4201 gcgacaccga gccggcgagc gtcgaacagc aacgcaacct cggcgcgacc gcgccgtcgc
     4261 tctacgacct gcgcaacttg ttccaggtca acgtggagga aggccgtcac ctgtgggcga
     4321 tggtctatct gcttcatacg tacttcggcc gtgacggtcg cgaggaagcc gaggagctac
     4381 tgatgcgccg ctcgggcgac gtcgacaagc cgcgcatcct cggcgcgttc aacgagccga
     4441 cgagccattg gctctcgttc ttcatgttca cgatgttcac ggaccgcgac ggcaagtatc
     4501 agctcgccgc gcttgccgag agcggcttcg atccgctcgc gcgcacttgc cgcttcatgc
     4561 tcaccgagga agcgcatcac atgttcgtcg gcgagaccgg cgtacagcgc atcgtgaagc
     4621 gcgcgtgcga gctgatgaag gagccgggcg gtgatgtccg cgcgctcggc ggcgttgatc
     4681 taccgacgat ccagaagtat ctgaacctct ggtactcgct ctcgctcgat ctgttcggtg
     4741 gcgaagtgtc gtcgaacgca gccgacgcat tcgcggcggg tctcaagggc cgttacaaag
     4801 aggaccgcta cgaggagcac aagctcatcg gctcgcattt cgcgatggac gtgctcgagg
     4861 acggcaaggt cgtccacaag gacatcgcga tgcgcaacgc gctcaacgaa gtgctgcgca
     4921 gcgagtacat caaggactgc cagcgcggcg tcgatcgctg gaacaagacg atcaaggacg
     4981 agggcatcga cttcgagcta cggctgccct cgaacaagtt ccatcgccag atcggcatgt
     5041 gggccggtca ccggttctcg cccgcaggcg agatgctcga cacggcgacc tgggatgcgc
     5101 ggcgtggtga gtggctgccg aacgacgccg acgagacgta catcaagagc ctgatgaagc
     5161 cggtgcacga gcgcggcaag atggcgaact ggatcgcccc gccggcaaag ggcatcaacg
     5221 gcctgcccgg cgacttcgag tacgtccggc tctgatcacg cgtcgggtgg cagcagctgt
     5281 tcgagctgcc acgtgtgaaa cgcggctctc gcctcggcgc gccgcagctc gacgacatat
     5341 tgccgcgcgt agatcacggt gacgccgagc gcaaaggcgc agctgatgat cagatagagc
     5401 tgcgtcagcc cgggcggcag cgcgtggagc tgcggcacga tcgtcacacc gccgtcgcga
     5461 aacacatgaa tcggcgggat cacgtgcaac cactcgagga cgaatggcaa tagcagcgtg
     5521 atgaacgtgc cggtcgatag cgcgaggccg tgctgccacg tcacgacgta caaccggtac
     5581 gcaagcagca cgagcgtcat cgcgaccggc aagacgccga ggaggcccat cgagcgactc
     5641 atgatcgcga gcgcccccag cgtcatcaca tacgtcatcg cgcgctgcca gacgtagatc
     5701 tggccgcgtg acagccaatg gaacagcagc gcaccgacga tgaatccgct gaacgcgccg
     5761 agcgcgcccc aatggcggac gccgagcacg gctgcgaacg gaatgaatgt gaagaacgcg
     5821 aacatcacga ggatgccgct cttcgccatg cgcatgctgc gttcgagcca gcgccgctcc
     5881 atcgccgcct gggcggcggg cggcaagtgg tttggcacct cgtgcaacag gcgagtgagc
     5941 gcacgcatcg cgcgcacgtt gccgggctcc atgccgagtg cgcggccgac gtgacgtgct
     6001 gcttctacgc gcgcatcagg cgtcggcgcg acctgtgcgc gctcgagcgc ttgctcggct
     6061 tggtgtgcgt gctcgtcggc cgcgcgcctg cgtgcctcga ggtcgcgatc accgtcgagg
     6121 aagcgctcga tggcgtcgtg cagctcgcgc gcgcttgcgt accgatcttc tggctctggt
     6181 tgcgtcgcgt ggctcgcgat cgccacgagc tcgggtgggg catcgcggtc gcacgcgagg
     6241 atctcaccga ggatcaggcc gagcgagaac acgtcgctgc gtgcatcggc atggcccatc
     6301 ctctgctcgg gtgccatgta gcgcggcgtg cccatcacag cgcccacgcg cgtcggcgtg
     6361 tcggtatcgg gaacctcgac gccctcggca agcggcgcgt cggcccgatc cataaccttc
     6421 gcgatgcccc agtcgagcac gtacacctcg ccgaagtcgc cgagcatcac gttcgacggc
     6481 ttcagatcgc gatggacgac gccgcgcgag tgcgcgaagt cgacggcgag gcacacgctc
     6541 gagaacgcgg taagcaaacg ccggcgcgag aaccgcgtgc ggtcgccgcc ttcgatcgcg
     6601 gtcagtgcct cctgcagcgt cgcaccgcgc acgcgcttca tcgtgaagta cagatcgccg
     6661 tcgcccgtcg tgcccaggtc gtgcaccggc acgatcgcag gatgctcgag ttgtccctgg
     6721 agccgcgctt cgcgaagaaa ccgcgcgcgg tactccgggt cgtcgcgcag cgagccgcgc
     6781 agcatcttca ccgcgacctg tcgcgcgatc cgcgagtcct tgcacagccg gatctcgccc
     6841 atgccgcctt tgccgagcag cgcgaccgac gcataacgat cggcatctgc gaccagcggt
     6901 gcgtcgtcga cattgacggg ggttgttcgt acgtcgtcga cgtgcgttgg actcaggtca
     6961 cgcgtgtcgt cgtcccgatg catcccccat tgtccgccgg ttcggtggtc agcttctagc
     7021 gaggagcgtt tcgtcgaaac gcgtcgcctg agcgtcatta tttgaacgtc aggtcctgcc
     7081 gactgacacc gcagaatcgg ccgatttcat cagcttgtac gactggtccg accattgctg
     7141 aatagagcga tcgcctatgc gttgcgtgat catcattctc gtactgctcg tcgcaacgcc
     7201 gcgcgcgttc gccgacgaag cgaagctcgc cgagaagacc cgtgtccccg cgtcgtatgc
     7261 ggggctcggc atcgaggcca acgatcagcg ccagggccgg cttggcttct ccggtgatgc
     7321 gggtctccgt ctcgacaact cgccgctctt tctgcgtgcg cggctgacgt tcgggctcag
     7381 tggcgccgac ggccactacg agcagctgcg cgttggactg gagggacgtt cgtgcgggcg
     7441 catgtgggtg tgcgtcttcg cggggctcga cttcggcggc cagcgcgacc atgccgtcgg
     7501 tgcgccgttc aacttccaag ggtggctcga caacgagccg ccgccgatga cggtcgtcga
     7561 ttcgctcgat gtgctactcg ttccgcgcat cggaattgaa acgccgacga agtttcgcgc
     7621 gcggatcgcg ctcgatgcgg tgatgttcaa ggagctcgaa gggcagggaa cgaccggcgt
     7681 cgcgtggaca ccctcgatca gcgccgcgta cacgttctag tcgtcgtccg agacgaatgc
     7741 gttctcgagc ggcttcaggt tctcggggag cggcgctgga atgcgggcct cgaagtcgac
     7801 ccacactcgt gtgtcttcgc ggcagtcgta cgagcggcag gtcgcggggc gatagtcata
     7861 ggcggtgcac gcacccttgc tgtccatgca cgcgcacttc ttcgtgatcg gatcgcgcgc
     7921 aagcaagtac ggctgatcga cgatcacggg cagcttgcgc tcggcgaggt cctgcggtga
     7981 cagcacgacg tcgagcgagc agcagcgcgc gcgacacaga tgcaggcgtg acacgcagtc
     8041 gatgacggga ctctcgacca cgtacttgtc gggctcgagc gagagccgga tcttgatgcc
     8101 gcgatcggat tggattcgaa agcggcgctg gacctcgtcg tctgcgagca gcccgaacgg
     8161 tgcgtcggcg tacttcacga gcagcgcgag cagctcgtcg gccactggat ccggatcgct
     8221 gctcattcgt cctcgggatt gacgaacgcg ttctcgagcg gcttgagatt ctcggggagc
     8281 ggcgccggaa tgcgcgcggc gtagtcgatc cagacgcgcg cgtcttcgcg gcaatcgtag
     8341 gtgcggcacg tcgcggggcg atagtcgtag gcggtgcatg cgcccttcgc gtccatgcac
     8401 gcgcacttct tcgtgatcgg atcgcgcggc aacaggtacg gctgctcgac gagaaatggc
     8461 agcttgcgct cgacgatgtc ctgcggcgat agcgcgactt cgaacgagca gcagcgcgcg
     8521 ccgcacagat ggatgcgcga cgcgcaatcg atgtccgggc tgtcgacgac gtacttgtcg
     8581 gtcgcgagtg agagccggat cttggtgccg cgatcggcct ggatcttggc ggccatgcgg
     8641 cggaagcgtt cgggcaactg tccgcgcatg atgagcgcat cgagcagcca ctcgaagcgg
     8701 cgcagcgctt cgtcgtgatt cggcagttgc ggcagatcgg aggcgagctt ctccatcagt
     8761 gcgagcagct cgtcggggac cggatccgga tcgctgctca tcgattggag tgtaacgtcc
     8821 aaccgcagag cttgtagagc acacgcgcgc cgacgttcgc gtcccactcg tcgccgtcgg
     8881 ggccggggac gacctcgttg aggtcgaagc cgatgatcgt gtggcccctc tcgacgacgc
     8941 gcttgatcaa atacgcggcc tgctggaacg acagcccacc gggcacaggc gtacccgtac
     9001 tcgggcagag cgcagggtcg aggccatcga tgtcgaacga cacgtagacc tgacgcggca
     9061 ggtccttgac gatcgcgtcg cagatgtcgc gccaggtttc gccagcgaac ccacgctcgg
     9121 cgagcgatgc atcgtgatgc acgaccacgc ggccattcgc ggcggcaacg gcatcggcct
     9181 cgcgcttcgc gaagtcgcgg ataccgacct gcacgagccg cttcacttgc ggaatcttct
     9241 tgatcacgtt gtacatgatc gatgcgtgcg accactcgaa gccttcgtag gcatcgcgca
     9301 gatcgcagtg cgcgtcgaca tgcaggatcc cgaagctgtc gtagctctcg gccatccgcg
     9361 cgatcgcgcc gaacggcacc gagtgatcgc caccgacgac gccgacgagc ttaccggcct
     9421 tcagcaggcg atcgacttgt tcgtaaacgt ggcgattgag acgcgcaccg gcttcgttga
     9481 cccgcgcgag cgattgcgcg agtgctgtgt cgtcgccaat gcggccgccg acctcgatga
     9541 ttttctcggc atcgacgcgc gcgctcatgt tccagcgcac gacctcgtcg ggaatgtcga
     9601 gcatcgtgat gccagcgcgc cacggctcgc cgagatcggc gtcgtagaga tcgagctggt
     9661 gactcgcaga gcgaatcgcc gccggaccgc gcgatgtgcc ggggcgatac gacgtcgtcg
     9721 cgtcccacgg caccggcacg atcacgagcc gcgcctcgtc gaccgtcgtc ggcagcccga
     9781 acagaccggc gtcggcgggt ggcgccgcgt cggcgtcgtg ctcctgcgtg ctcactcgcc
     9841 gccctcttgc ggatcggagg aacgcgtgtc gcggtcgtcc tcgtcgcctt ccgcgggcgc
     9901 tttgacgtcc ttctcgtcct cgccttcctt catctgctgc gactcggacg cggcggggct
     9961 ctgcggagct ttcttcttgc cgcagccccc gaaagcgagt gtcgcgaggc tgacgacgat
    10021 cactagtgcg atgcgcatgg cggcatacta cttcttgcgc tggagcaagt cgccgagcgt
    10081 gccgagcttc tgcggcgccc tcggtacagc agcgacgtcg tcggggctcg catccgtcga
    10141 cgcggcgagc gacagtgaca gccggcgctt gtcgtggtcg accgcgagca cggtcgcttc
    10201 gacctgctga ccgatcgaga ccacttcctt cggatgattg atgcggcggc cggcgccaag
    10261 ctcgctgatg tgaacgaggc cttcgacgcc gggcgcgatc tcgacgaatg cgccgaacgg
    10321 ctgcagccgc gtgatcgttc ccttcacgcg cgcgccttcg ccgagtgatg ctgtcgcgtc
    10381 gcgccatgga tcgttcgcga gcgccttcaa gctcaggctg atccggtcac ccttctcgcc
    10441 cggctcgatc ttgagcaccg caacgtcgag ctcctggccg agcgacagca cgtcctcggg
    10501 cttctcgacg cgcgcgaacc cgagctccga cacgtgcagc atgccttcga tgccaccgag
    10561 atcgacgaac gcgccaaacg gcttgaagcc gacgaccttg ccgcgtagta cggcaccggg
    10621 cgcgagcttc ttgcgcgtct cggtcgcgag cttctcttgc gcttcctcga cgagcgcgcg
    10681 gcgcgacagg acgaggttgc gcggctcgta gcgcgtgatc ttgaacgtca gcttctgccc
    10741 gacgtacgtc gcgagatcct cgacgaagcg attgtcgacc tgcgacgccg ggcaaaaccc
    10801 acgcacgccg gcgacgtcga cggatacgcc gcccttgatg acctcggtca ccgtgccttc
    10861 gacgggaatg ccgagctcga acgcctgctg cagctcggcg cgcgcttcgg ggccgcgcgt
    10921 caccttcgtg cgcagcatca gtacgccgcc ggtatccgag acgatgcgcg cttcgatcac
    10981 gtcgccgact ttgacgagca gcttgccgtc cttgtcgctg acctgattct tctcgagctg
    11041 gccttccgcc ttgccgccga gatcgacgaa cacggcttcc ttgccgatcg agatcacctt
    11101 gcctttgatg acgtcgccga tcttcgggcg cttcgccttc ggcttgtcgc gttcgctctc
    11161 cgcaaacatt gcggcaaagt cttcgtcctc ggtggccatg ggtggcgcac tctacgcgac
    11221 gagacccgca tcgtaaaggc gatgcgaacg gcgccagtac cgcggcttgc ttagttcgac
    11281 gcgcggactt ccgcgggctt cgccgacgac ggcggcggga cgacgaggtc gtcgatcacc
    11341 gtgcgggcgg cgttcgtgag gccgagcgac ctcgcgctga ggaagccgac cttgcgcatc
    11401 gcccagtccc acacgcgcgt cgggaacatg cgtgcgaaca ggatcacgaa gttcgtcgag
    11461 aatggcgcga cgtagcgcgc ggccggacgg cgagcattga ccgcgcgtgc gatcgccttc
    11521 gcgatcacga tcgggttcga cgcgaaacgg tcagccgtct tcgacatctg ctcgtacttc
    11581 gcgaccgcga ccttgtaagg cgtgttctcg agctgcttga ggttgccggc cgcagtcgcc
    11641 tcgaagttcg tgcggatcac gccgggctcg atcatcgcga cgtcgatgcc gaacggacgc
    11701 agctcgtacc gcatcgcatc cgacagcgac tcgaccgcat acttcgtcga gttgtagacg
    11761 ccgaagtagg gcagcgtgat gcggccgccg acgctggaca cgttgatgat gcggccgctg
    11821 cggcgctgca tcatggcggg caagaacgcg cgcgtgacgt tcatgaggcc gaacacgttc
    11881 gtctcgtact ggcgacgcat ctcggagtcg gtgatctccg acgtcgggcc gagcacgccg
    11941 aagcccgcgt tgttgacgag gacatcgaga ccacgaccac tcgtcagcgc atcgactgca
    12001 cgcgcggccg cttcgatcga tgcgagcgac gtgacgtcga gcagaagcgt gtcgagcttg
    12061 cccttgccga ggatc
//
LOCUS       DASUFX010000156        10516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_718709,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000156 DASUFX010000000
VERSION     DASUFX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10516
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718709"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1498
                     /locus_tag="VFV99_06225"
     CDS             41..1498
                     /locus_tag="VFV99_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058935.1"
                     /translation="MRFALVFVAVAACDGGPKLTPDAGADSNAMADAPVDAPSGILGT
                     WRDTFITVSGTQSMSACSAAPSALVVDATTAAVTPYSGACKTDGSFHIMAPANLGAYY
                     LRVAGALYETTKHADLDLSTDHLGRNDVAAITGVDLTLSMTGLDAWGAGDVLMAFGAN
                     IGYFQNLSFTSGGPVNGSTNLTGTAPWAGYKIDAAKSDSLQVVQLGVHSTAGGLSYLS
                     LDRSFDVSPFTMTNNTAQNVQGAFTPPSAGTLALNVNVNSFNQFATVASPDVSAKTIA
                     GSAYAAPSAEVIPSPSLISFARDSTGLSALNFGTLDYGDPFPTAWSRFVKVTEAFSVP
                     YTWNNISGTMNAFMTRVMTKAEAEANIIDARLGPPLNPTFDGANAFTATNISPVPMVS
                     WQAPSLGTATDYELVVYEVQINGTSLKFSPTLRLSTKQTSVRVPAGYLLGQRQYMFVI
                     RARSRDGEDLYGTPLRMGESTSTADTLTALVTTDS"
     gene            complement(1501..2325)
                     /locus_tag="VFV99_06230"
     CDS             complement(1501..2325)
                     /locus_tag="VFV99_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058936.1"
                     /translation="MWKRVAKVIVGVLAGWLVVLWVLGIAIGSHQERKTVDRLAESLQ
                     AIVTIDDSNLALIRGRWSMERLAVRHDDPLGKLSIDVAGVRCELGPLGWALVDRDCSE
                     LAVTGVRMEVSSTQLFKVPRPKRKPIHAEHIVIDDAVLVFQPSAVMPSLGRIEIAIEH
                     AESGPTTLRTPLSWLLTLETLRAKLELPAGITLHLSYAKGVLTASGSLFGSAPVELPV
                     QLPVVALAHDTHEEMQQLIALGKDIAERLVAKRATDWLESKLKRPAIPPPSGSAAP"
     gene            complement(2384..4357)
                     /locus_tag="VFV99_06235"
     CDS             complement(2384..4357)
                     /locus_tag="VFV99_06235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5058937.1"
                     /translation="MAQASKYTGEEIQVLEGLDPVRKRPGMYIGGTGKDGYHHLLWEV
                     VDNSIDEVINKHATKVEVTLHKDGKTCTVEDNGRGIPVDVMKKFKKSALEVIFTTLHS
                     GGKFERGKSYAVSGGLHGVGAAVVNALSSELVATVKRDSERFEMTFQRGEPKGKLKKL
                     GPARGSGTTVTFSPDDEVFGGKLHFDAELIAERLEAKSYLHGGLEIIFVDETGTSSPT
                     GKAGGGPSMTHRFVHPQGIAEYLPKLVTERGKNPVPANGGVFYMEKRDDEAQLGIELA
                     LQWTESPDDLFKTYVNSVPTPDGGTHDAGLRSAIVKAIRNYIATHKLDPKGVTLTAED
                     IREGVVAVLSTYVHDPQFQSQTKNRLNNPEVAAQVEALVRPALENYLNANPNWGQAIV
                     ARTIIAARAREASRAAQQVTRKTAVSHRLNLPGKLADCSSTDPADSELFIVEGESAGG
                     SAKQGRDRKTQAILPLKGKVLNTEQASGQKLLANKELADIVSALGCGMGETFDLGKLR
                     YGKIILLMDADADGHHISTLLMTFFFRHMRPLIDAGHVFLGQPPLYRIDIGKQTLWAL
                     DDLHKERLLKTHAKSNSKPSITRFKGLGEMNPETLKQTTLDPKTRQVLRVTVKDGEQL
                     LTDQVISDLMGKDVAARFKLITENAAVIGELDV"
     gene            complement(4359..6827)
                     /locus_tag="VFV99_06240"
     CDS             complement(4359..6827)
                     /locus_tag="VFV99_06240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5058938.1"
                     /translation="MAKKSDKKASPKGDGASKKSGGKKAKAAAGGGGGGGGGGVAAGG
                     GGDGMGMPGMHDASLEAEARRRYLNYALSVITSRALPDVRDGLKPVQRRILYAMLNDN
                     HLRPDAKHRKSAKVVGDVIGRYHPHGDTAVYDAMVRMAQDWALRVPLVDGSGNFGSLD
                     GDEAAAYRYTECRLAPPAMELLRELDFNTVDMRANYDGTSEEPVVLPARFPNLLVNGS
                     TGIAVGMATNIPPHNLKEIVEALIALSKDRKIEHVTLMKYINGPDFPTGGQLMNSKVE
                     LRQIYKEGAGTIRIRGEYKTEEKKRGGQDIVITSIPYAMSKADLVQKIADVIVSRKMP
                     FLLDVRDESTTDVRIVLELKKDADPNLVMGYLYKHTPLQNTFHVNLTCLVPPELLDDG
                     SVPSGAPPQPKKLGIKEMLEHFLDFRIITTTRRFEYQLAQLKKRIHILDGFVTIFDAL
                     DETIKIIRASDGKEDAAQKLIKRFKLDEIQVDAILELKLYKLAKLEINVIREELEEKS
                     KEAKRIQKILNDEEKLEDVVRTELEEIAKEYGTPRRTKTAAAGAEEMEFDENAFIVEE
                     DANVVVSKDGWIKRVRELKDPAQTRTREGDAVAHVLPGSTREKVIFFTNRGSAYVIKI
                     NDIAPTTGYGDPAQKYFKFGDGEQIVAAMTLDPRAMVPPTLLAVSKHGFGLRFATAQH
                     TEVTTKAGRRYARPKEGDEILNVIPCNDSDVVVAATHRGHILWCKADEIAKLEGAGRG
                     VTVIKVTPGDHVVGFIAGGKGDVLQLESEKGGRDYSEKADPKQVTSRGGKGRQVIKKG
                     TLVAVPKPVTIQPLANVEGSPKVN"
     gene            7080..8378
                     /locus_tag="VFV99_06245"
     CDS             7080..8378
                     /locus_tag="VFV99_06245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5058939.1"
                     /translation="MLLILGALVLVNLYVFVWDKKTGIKAVQKQAEAVTPSMAVPSAP
                     LAVAPPVAPDAAGNAAAPAPVGPPGAIEGTVGKSDTLGKVLKKSGLSAGEADEVIRSL
                     AGVLDFKKIRPGQTFKIERGPDGRVKLFELELSKIQKVRAEREPTGELVGKADDSQTR
                     LELKAVTGTIDSSLYAAIKNSGETAALVDFFVDVFAYDIDFYNDTFDGDTFRVVVEKR
                     YKDQEFIQYGRILAAEYKGKAGTFKTFFWNGKYYDESGESSERGMLKTPLKFARVSSG
                     FDRKRMHPVLHTVRAHLGIDYAAPVGTPVWAAAPGVVTVRAPSGGAGNLVMIRHDSGI
                     ETAYMHLSKFASIKVGQRIDAKTVIGFVGTTGLSTGPHLHFGVKKNGEYIDPTKLAPM
                     RGKPVPRSQIDAFRAEAAKLEGKMTDATIGLADPRLPRHA"
     gene            8382..8822
                     /locus_tag="VFV99_06250"
     CDS             8382..8822
                     /locus_tag="VFV99_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058940.1"
                     /translation="MVKLLVGLLKGAAIGGAVGYGAVALADATGFSNAWLTVGLIGAL
                     VGLFVGKPLWALLKDKNQTNIIALIKAAFGFGVGCGLYALLAKAWAPDPKLLEVSGYP
                     LLYWPPTVGALIGGLYGAFVELDDSIGDDGSGKKKPAAAKQLDK"
     gene            complement(9068..10459)
                     /locus_tag="VFV99_06255"
     CDS             complement(9068..10459)
                     /locus_tag="VFV99_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5058941.1"
                     /translation="MLARCAAFVTLVAWCATARGEAPIVGSRTARSIGRAGVGTVSDD
                     GGGALLANPAAIARRESTRIQFGLLFLDDEMFWVAAKTAPAALDQSSSRLVPQIAIEG
                     AICNWVIGIGAGTSVRSERAFRRPGRLPPSDFGNSFEYRYTGLAGAIRRDTLTAAGAH
                     RLTDSVAIGVSLGTSRVSIRESRRLWAGDVDRVVLGVPQPDLLGDPAHDVELALTGTD
                     NFSPSATAGVLIAPEDSRIELGAAVMWSAPARVTGDVESTNEDPDAKVRADTNAAHAR
                     LEVEQPIIVHTGVRWLGERVIGEINGDLYWFPQRAAATEWNLSGVMLTDITTPTQPES
                     VELPKLASRISSRTHGALRGAVDVELIGGFLWATAGYAFTTSGTPDARMSPTFGDLGG
                     HTVGLGLEATTGGFTVTLGWARTWSIKEAEPVSRWRLDNPFGSGDGMIASGTYDGSTD
                     MIGLSVDADLDAE"
BASE COUNT         1685 a         3512 c         3378 g         1941 t
ORIGIN      
        1 acctgtcggc ctgtcgcacc tcgtgatacg tagagccact atgcgttttg ccttggtctt
       61 tgtggcggtg gcggcgtgtg acggtgggcc caagctcacc ccggacgcgg gcgcagacag
      121 caatgcaatg gccgatgcac cggtcgatgc tccgtccgga atcctgggca cgtggcgcga
      181 cacgttcatc accgtcagcg gcacgcaatc gatgtcggcg tgcagcgcgg cgccgtccgc
      241 gctcgtggtc gatgcgacga ctgcggcggt cacgccgtac tcgggcgcgt gcaagacgga
      301 tggctcgttc cacatcatgg cgccagcaaa cctcggcgcg tattacctgc gggtcgcggg
      361 cgcgctctac gagacgacga agcacgccga cctcgatctg tcgaccgacc atctcggccg
      421 caacgacgtc gccgcgatca ccggcgtgga cctcacgctc agcatgaccg gcctcgacgc
      481 gtggggcgcg ggcgacgtgc tgatggcgtt cggcgcgaac atcggctact tccagaacct
      541 gtcgttcacg tcaggtggtc cggtgaacgg cagcacgaac ctcaccggca ccgcgccgtg
      601 ggctggctac aagatcgacg cggcgaagtc cgacagcctg caagtcgtcc agctcggcgt
      661 gcactcgacc gccggcgggc tctcgtatct gtcgctcgat cgttccttcg acgtctcgcc
      721 gttcacgatg acgaacaaca cggcgcagaa cgtccagggt gcgttcacgc caccatccgc
      781 gggcacgctg gcgctcaacg tcaacgtcaa cagcttcaac cagttcgcga cggtcgcgag
      841 tcccgacgtc agcgcgaaga cgattgcggg cagcgcgtat gctgcgccgt cggccgaagt
      901 gattccgtca ccgtcgctga tctcgttcgc gcgtgacagc acgggcttgt cggccctgaa
      961 cttcggcacg ctcgactatg gcgatccgtt cccgacggcg tggagccgct tcgtcaaggt
     1021 taccgaggcg ttctcggtcc cgtacacgtg gaacaacatc agcggcacga tgaacgcgtt
     1081 catgacgcgc gtgatgacga aggccgaagc cgaagcgaac atcatcgacg ctcgcctcgg
     1141 cccgccgctc aatccgacgt tcgacggtgc caacgcgttc accgcgacaa acatcagccc
     1201 ggtgccgatg gtcagctggc aagcaccgtc gctcggtacg gcgactgatt acgagctcgt
     1261 cgtctacgag gtgcagatca acggcacgag cctgaagttc agcccgacgc tccgcctgtc
     1321 gacgaagcaa acgtcggtgc gcgtgcccgc cgggtatctg ctcggccagc gccagtacat
     1381 gttcgtgatc cgcgcgcgct cgcgcgacgg cgaagatttg tacggcacgc cgctgcgcat
     1441 gggcgagtcg acgtcgaccg ccgacacgct gaccgcgctc gtcacgacgg actcgtaacg
     1501 ctacggcgcc gccgaaccgc ttggaggcgg aatggccggg cgcttcagct tcgattccag
     1561 ccagtccgtg gcgcgcttcg cgacgagccg ctccgcgatg tctttgccga gcgcgatcag
     1621 ctgctgcatt tcttcgtgcg tgtcgtgcgc caacgcgacg accggcagtt gcacggggag
     1681 ctcgaccggc gccgacccga acagcgaccc cgacgcggtg agcacgccct tcgcgtacga
     1741 caggtgcagc gtgattccag ccggcagctc gagcttcgcg cgcagcgtct cgagcgtgag
     1801 cagccaggac agcggtgtgc gcagcgtcgt cggcccgctc tcggcgtgct cgatcgcgat
     1861 ctcgatgcgg ccgaggctcg gcatcaccgc gctcggctgg aacacgagca ccgcgtcgtc
     1921 gatgacgatg tgctcggcgt ggatcggttt tcgcttcggc cgcggcacct tgaacagttg
     1981 cgtgctcgac acctccatgc gcacgccagt gaccgcgagc tcgctgcaat cgcgatcgac
     2041 gagcgcccag ccgagcggcc caagctcgca gcgcactccg gcgacatcga tcgacagctt
     2101 gccgagcgga tcatcgtggc gcaccgcgag gcgctccatc gaccaccggc cgcgaatcag
     2161 cgcgaggttg ctgtcgtcga tcgtgacgat cgcctgcagc gactcggcga gccggtcgac
     2221 cgtcttgcgc tcctggtggc taccgatcgc gatgcccagg acccacagca cgacgagcca
     2281 gccggcaaga acgccgacga tgaccttcgc gacccgcttc cacacccgcc gatcctagcg
     2341 tgtctccgcg cctgaggcct gagggcccga ggcctgaggc ccgctagacg tcgagctcgc
     2401 cgattacagc ggcgttctcg gtgatcagct tgaatcgcgc agcaacgtct ttgcccatca
     2461 ggtcggagat gacctgatcg gtgagcagct gctcgccgtc cttcaccgtc acgcgtagca
     2521 cctggcgcgt cttcggatcg agcgtcgtct gcttcagcgt ctctgggttc atctcgccga
     2581 gacctttgaa ccgcgtgatg ctcggtttgc tgttgctctt cgcgtgcgtc ttgagcagcc
     2641 gctctttgtg cagatcgtcg agtgcccaga gcgtctgttt gccgatgtcg atgcgataca
     2701 gcggcggctg gccgaggaag acgtggccgg catcgatcag cggccgcatg tgacggaaga
     2761 agaacgtcat cagcagcgtc gagatgtgat gcccgtcggc gtcggcatcc atcagcagga
     2821 tgatcttgcc gtagcgcagc ttgccgagat cgaacgtctc gcccatgccg cagccgagcg
     2881 cactgacgat gtccgccagc tccttgttcg cgagcagctt ctgaccactc gcctgctccg
     2941 tgttgagcac cttgcccttg agcggcaaga tcgcctgcgt cttgcgatcg cggccttgct
     3001 tcgcggagcc acctgccgat tcaccctcga cgatgaacag ctcgctgtcg gccggatcgg
     3061 tcgacgagca gtcggcgagc ttgcccggca ggttgagccg gtggctgacc gccgtcttgc
     3121 gcgtgacctg ctgcgctgca cgcgatgctt cacgtgcacg cgccgcgatg atcgtgcgag
     3181 cgacgatggc ctggccccag ttcgggttcg cgttgagata gttctcgagc gcggggcgca
     3241 cgagcgcctc gacctgcgcc gcgacctcgg ggttgttgag ccggttcttg gtctggctct
     3301 ggaactgcgg gtcgtggacg taggtcgaga gcacggcgac gacgccctcg cggatgtctt
     3361 ccgcggtgag cgtcacgccc ttcggatcga gcttgtgcgt cgcgatgtag ttgcggattg
     3421 ccttgacgat cgcagagcgc aagcccgcat cgtgcgtacc gccgtcgggc gtcggcaccg
     3481 aattgacgta cgtcttgaac aggtcgtccg gcgactcggt ccactgcagc gcgagctcga
     3541 tgccgagctg cgcttcatcg tcccgcttct ccatgtagaa cacgccgccg ttcgccggca
     3601 ccgggttctt gccgcgctcg gtgacgagct tcggcaggta ctccgcgatg ccctgcggat
     3661 gcacgaaccg gtgggtcatc gacggccccc caccggcttt gccggtgggt gatgaggtgc
     3721 cggtctcgtc gacgaaaatg atctcgaggc cgccgtgcag gtagctcttc gcctcgagtc
     3781 gctccgcgat cagctcggcg tcgaagtgca gcttgccgcc gaacacctcg tcgtcgggtg
     3841 aaaacgtcac cgtcgtgccg ctaccgcgcg cggggccgag cttcttcagc ttgcccttcg
     3901 gctcgccgcg ctgaaacgtc atctcgaagc gctcgctatc gcgcttcacg gtcgcgacca
     3961 gctcactcga cagcgcgttg acgaccgccg cgccgacgcc gtgcagaccg cccgatactg
     4021 cgtagctctt gccgcgttcg aacttaccgc ccgagtgaag ggtcgtgaag atcacctcga
     4081 gggcgctctt cttgaacttc ttcatcacgt cgacgggaat gccgcgcccg ttgtcctcga
     4141 cggtgcaggt cttgccatcc ttgtgcagcg tgacctcgac cttcgtcgcg tgcttgttga
     4201 tcacctcgtc gatcgagttg tcgacaactt cccagagcag gtggtggtag ccgtccttgc
     4261 cagtaccacc gatgtacatg cctgggcgct tacgaacggg atcgagaccc tcgagtacct
     4321 ggatctcttc gccggtgtat ttcgatgcct gcgccatctc agttcacctt cgggcttccc
     4381 tcgacgttcg cgaggggttg aatggtcacg ggcttcggca cggcaacgag cgtgcccttc
     4441 ttgatcacct ggcggccctt gccgccgcgc gaggtgacct gtttcggatc ggccttctcg
     4501 gagtaatcgc gaccgccctt ctcgctctcg agctggagca cgtcgccctt accaccggcg
     4561 atgaagccga cgacgtggtc acccggcgtc accttgatca cggtgacacc tcggcccgcg
     4621 ccctcgagct tcgcgatctc gtcggccttg caccacagaa tgtgacccct gtgcgtggcc
     4681 gcgacgacga catcgctgtc gttgcacgga atcacgttga ggatctcgtc gccttctttc
     4741 ggtctcgcat agcggcggcc ggccttcgtc gtgacctcgg tgtgctgcgc agtcgcgaag
     4801 cggaggccaa agccgtgctt cgagaccgcg agcagcgtcg gcggcaccat cgcgcgtggg
     4861 tcgagcgtca tcgccgccac gatctgttcg ccgtcgccga acttgaagta cttctgcgcc
     4921 ggatcgccgt aaccggtagt cggtgcgatg tcgttgatct tgatgacgta ggcgctaccg
     4981 cggttcgtga agaagatgac cttctcgcgc gtcgagccgg gcagcacgtg ggcgaccgca
     5041 tcgccttcgc gcgtacgggt ctgcgccgga tccttgagct cgcggacgcg cttgatccag
     5101 ccgtccttgc tgacgaccac gttcgcgtct tcctcgacga tgaacgcgtt ctcgtcgaac
     5161 tccatctcct cggcgccggc cgccgcggtc ttcgtgcggc gtggcgtgcc gtactccttc
     5221 gcgatctctt cgagctcggt gcgcacgacg tcttcgagct tctcttcgtc gttgaggatc
     5281 ttctggatcc gcttcgcttc cttgctcttc tcttcgagct cttcgcggat gacgttgatc
     5341 tcgagcttcg ccagcttgta gagcttcagc tcgaggatcg cgtcgacctg gatctcgtcg
     5401 agcttgaacc gcttgatcag cttctgcgcc gcgtcctcct tgccgtccga ggcgcggatg
     5461 atcttgatgg tctcgtcgag cgcgtcgaag atcgtgacga agccgtcgag gatgtggatg
     5521 cgcttcttga gctgagcgag ctggtactcg aaccgccgcg tcgtcgtgat gatgcggaag
     5581 tcgaggaagt gctcgagcat ctccttgatg ccgagcttct tcggctgcgg cggcgcgccg
     5641 ctcggcacgc tgccatcgtc gagcagctcg ggcgggacca ggcacgtgag gttcacgtgg
     5701 aaggtgttct gcagcggcgt gtgcttatag aggtagccca tcacgaggtt cggatcggcg
     5761 tccttcttga gctcgagcac gatgcgcacg tcggtcgtcg actcgtcgcg cacgtcgagc
     5821 aggaacggca tcttgcgcga cacgatcacg tccgcgatct tctggacgag atccgccttc
     5881 gacatcgcgt acgggatcga cgtgatgacg atgtcctgcc cgccgcgctt cttctcctcg
     5941 gtcttgtact cgccgcgaat gcggatcgtg ccggcgcctt ccttgtagat ctggcgcagc
     6001 tcgaccttgc tgttcatcag ctggccaccg gtcgggaagt ccggcccgtt gatgtacttc
     6061 atcaacgtga cgtgctcgat cttgcgatcc ttcgacagcg cgatcagcgc ctcgacgatc
     6121 tccttcaagt tgtgcggcgg gatgttcgtc gccatgccga ccgcgatgcc ggtcgagccg
     6181 ttgacgagca ggttcggaaa ccgcgccggc agcacgaccg gctcttcgct cgtgccgtcg
     6241 tagttcgcgc gcatgtcgac ggtgttgaag tcgagctcgc gcaagagctc catcgcgggc
     6301 ggcgcgagcc ggcactcggt gtaacgatag gcagcagcct cgtcgccatc gagtgaaccg
     6361 aagttgcccg agccatcgac gagcggcacg cgcagcgccc agtcctgggc catgcgcacc
     6421 atcgcgtcgt acaccgcggt gtcgccgtgc gggtgatagc gaccgatcac gtcaccaacg
     6481 accttcgcgc tcttgcgatg cttcgcgtcc gggcggaggt ggttgtcgtt cagcatcgcg
     6541 tagaggatgc ggcgctgcac cggcttcaat ccgtcgcgca catccgggag cgcgcgagac
     6601 gtgatgaccg agagcgcgta attcagataa cgacggcgtg cctcggcttc gagcgacgcg
     6661 tcgtgcatgc ccggcatgcc catgccgtca ccgccaccgc ccgcggccac tccgccgcct
     6721 ccgccaccgc caccgccacc agctgcggcc tttgccttct tcccaccgga tttcttgctg
     6781 gcgccatcgc cctttggcga cgccttctta tcggacttct tggccatgtc ttgctcggat
     6841 tgggtcgcac cttgccccga gttggagaaa agttccagct gctcacgcga cggcagatcg
     6901 ccaggggtgc ttgccatgat taggagacgc tccgaatatg gattgttggt ccgacaccaa
     6961 gaggcgtgat attgctcagc ctctcgaggg ccatggtccg cagacgtacg tccccgcgca
     7021 agaaaaggac tgcctaccct gcattgggag cggctcccgc caagcgcagc aacgcgtggg
     7081 tgctcctcat tttgggcgcg ctcgtcctcg tgaacctgta cgtcttcgtc tgggacaaga
     7141 agaccggcat caaggcggtc cagaagcagg ccgaagcggt caccccgtcg atggccgttc
     7201 cttcggcgcc gctcgccgtt gccccgccgg tcgccccgga tgctgccggc aatgccgcag
     7261 cccctgcgcc ggtcggcccg ccgggtgcga tcgagggcac ggtcggcaag tccgacacgc
     7321 ttggcaaggt gctcaagaag agcggcctgt ccgcaggcga agccgacgaa gtgatccgct
     7381 cgctggccgg cgtgctcgat ttcaagaaga tccgccccgg ccagacgttc aagatcgagc
     7441 gcggtcccga tggccgcgtg aagctgttcg agctcgagct ctcgaagatc cagaaggtgc
     7501 gcgccgagcg cgagccgacc ggcgagctgg tcggcaaggc cgatgacagc cagacgcgtc
     7561 tggagctaaa ggcggtcacg ggcacgatcg attcgtcgct ctacgcggcg atcaagaact
     7621 cgggcgagac cgccgcgctg gtcgacttct ttgtcgacgt gttcgcgtac gacatcgact
     7681 tctacaacga cacgttcgac ggcgacacgt tccgcgtcgt ggtcgagaaa cgctacaagg
     7741 accaggagtt catccagtac ggccgcatcc tcgccgccga gtacaaaggc aaggcgggca
     7801 cgttcaagac gttcttctgg aacggcaagt actacgacga atccggtgag tcgtcggagc
     7861 gcggcatgct gaagaccccg ctcaaattcg cgcgcgtctc gtcgggattc gatcgcaagc
     7921 gcatgcatcc ggtgctgcac acggtgcgcg cgcacctcgg catcgattac gccgcgcccg
     7981 tcggcacacc ggtgtgggcg gctgcgccag gcgtcgtgac cgtccgcgca ccgagcggcg
     8041 gcgcgggcaa cctcgtgatg atccgtcacg acagcggcat cgagacggcg tacatgcacc
     8101 taagcaaatt cgccagcatc aaggtcggtc agcgcatcga tgcgaagaca gtgatcggct
     8161 ttgtcggcac caccggattg tcgacgggcc cacaccttca tttcggggtg aaaaagaacg
     8221 gggaatacat cgacccgaca aagctcgcgc cgatgcgcgg aaagcccgtg ccacgcagcc
     8281 agatcgacgc gttccgtgcc gaagcggcaa agctggaagg caagatgacg gatgccacga
     8341 tcggccttgc cgatccgaga cttcctcgtc atgcttgatc tgtggtcaag ctcctcgtcg
     8401 gactactaaa gggcgcggca atcggtggag ccgtcggcta tggcgcggtc gcgctcgcgg
     8461 acgcgacggg cttcagcaat gcctggctca cggtcggact gatcggagcg ctggtgggcc
     8521 tgttcgtggg caaaccgctg tgggcgctgc tcaaggacaa gaaccagacc aacatcatcg
     8581 cgctcatcaa ggcggcgttc ggcttcggcg taggctgtgg cctctacgca ctgctcgcaa
     8641 aggcctgggc gcctgacccg aagctcctcg aggtgtcggg gtacccgctc ctgtactggc
     8701 cgccgactgt cggtgcgctg atcggcggtc tctacggcgc gttcgtggag ctcgacgatt
     8761 cgatcggcga cgacggctcc ggcaagaaga agccggccgc cgcgaagcag ctcgataagt
     8821 agcggtccga gctacggatt tcgcgggagc acagcgtacg agcagatcct tggtcgcctc
     8881 gctgaggcgc gctcgtctcg actagccact ggctaaggca tcagccagtg agacactgca
     8941 agttccgcgg ggttgagcgc ggcgcgccgg ctgcatttaa tgccgccatc catgcgcgac
     9001 atccggctct tcatcgttgc ccacctgatg ctcgttgcgg tcgcgatcgt cggcagcctg
     9061 tagcgcgtca ctcggcgtcg aggtcggcat cgaccgagag gccaatcatg tcggtcgagc
     9121 catcgtaggt tcccgacgcg atcattccgt cgcccgagcc gaacgggttg tcgagccgcc
     9181 agcggctcac cggttcggct tccttgatcg accacgtgcg cgcccacccg agcgtgaccg
     9241 tgaagccgcc ggtcgtcgcc tcgagcccga ggccgacggt gtggccgccg agatcgccaa
     9301 acgtcggcga catccgcgca tccggcgtcc ctgacgtcgt gaacgcatag ccggcggtcg
     9361 cccacagaaa gccgccgatg agctcgacat cgactgcgcc gcgcagcgcg ccgtgcgtgc
     9421 gcgacgagat ccgcgacgcc agtttcggca gctcgacgct ctcgggttgc gtcggcgtcg
     9481 tgatgtccgt cagcatcacg ccgctgaggt tccactcggt tgccgccgcg cgctgcggaa
     9541 accagtagag gtcgccgttg atctcgccga tcacccgctc gccgagccag cgcacgccag
     9601 tgtgtacgat gatcggttgc tcgacctcga gtcgcgcatg cgcggcgttc gtgtcggcac
     9661 gcaccttcgc gtcgggatct tcgttcgtcg actccacgtc gccggtgacg cgggcgggcg
     9721 cgctccacat cacggctgcg ccgagctcga tgcgcgagtc ctcgggcgcg atcagcacgc
     9781 cggccgtcgc gctcggcgag aagttgtcgg tgcccgtgag cgcgagctcg acgtcatgcg
     9841 caggatcgcc gagcaagtcg ggctgcggca cgccgagcac gacgcgatcg acatcaccgg
     9901 cccacagtcg ccgcgattcg cggatcgaca cgcgcgacgt tccgagggat acgccgatcg
     9961 cgacgctgtc ggtgagccgg tgcgcgccgg cagcagtcag cgtgtcgcga cggatcgcgc
    10021 cagcgaggcc cgtgtagcga tactcgaagc tgttgccgaa gtcgctcggt ggcaaacgac
    10081 ccggccggcg gaacgcgcgc tcgctgcgca cgctcgtacc ggcgccgatg ccgatcaccc
    10141 agttgcagat cgcgccttcg atcgcgatct gcggcacgag ccgcgagctc gattgatcga
    10201 gcgcagcggg tgcggtcttc gcggcaaccc agaacatttc gtcgtcgaga aacaggagcc
    10261 cgaactggat gcgcgtgctc tcgcgccgcg cgatcgccgc aggatttgcg agcagtgcgc
    10321 cgccgccgtc gtcggagacc gtgcccaccc ctgctctacc gatcgatcga gcggtgcgac
    10381 tgcccacgat cggcgcttcg ccgcgcgccg ttgcgcacca cgcgacgagc gtcacgaagg
    10441 cggcgcagcg agctaacaca gcgggacgat atcgcgtcgg cgccgccgag tggtcggcgc
    10501 aggtgcacgc gatgca
//
LOCUS       DASUFX010000157        12314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1553687, whole genome shotgun sequence.
ACCESSION   DASUFX010000157 DASUFX010000000
VERSION     DASUFX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553687"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFV99_06260"
     CDS             <1..302
                     /locus_tag="VFV99_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5058942.1"
                     /translation="MYLGRIVELADAKVLYREPKHPYTQALLSAVPRVAVSGAKSKRE
                     RIILQGDVPSPLAPPTGCAFHPRCPVKDKPEACFKEVPKLRVLSNGSRAACHVAE"
     gene            320..3172
                     /locus_tag="VFV99_06265"
     CDS             320..3172
                     /locus_tag="VFV99_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5058943.1"
                     /translation="MQSRSGELAMRPLPAVLLDLHEDIVTGRLTLRRGKVSKSVDLIN
                     GNPVSTASTPRDETLGHFLTSTGVITEDQHREAVQRAAKADSKLGEALVAMQVLSVEQ
                     LIEQLGKQARHKLVQALRWPQGAWRFEEGREPIEGMQLRMIEVVLGGLRETAVEDLGR
                     LSRLDGMSFELTERGTRLKHELKKAFGERALAVLAAGGPITDIEKALGDRVQARVALD
                     SMLLCDAIVSKAKAVGLGAQLAKVKVAQVPAPSPAIEIEPDEPPPEGEEGEELFALLF
                     DEIEGGEDDHKTKDGEKPLDFELDAVEAVDDHNDSGVVSMVDVQETIQKRAKAQAARQ
                     AIIAEHQRIQGADHYAVLLIDRDATKDDIDGGYQVKLTLLQRKTDEVTDPQDRAKLDR
                     IITAYNTARATLLDPQKRAAYNRELAGGELVEGPPAIDTELQFRMAEELLAKKQWAQA
                     IGMLKTVISQSPNEADYHAALGWATWNAGNAQANAADEARNYLNHALSINPDHPAAHD
                     YKGRIDASLRVDDANAVFHLERALDLDPQRTEALATLENLLVARGELRRFERIMKRLL
                     FRLRGKTHSVEASAWARLTRLYLDHLDDPGAAAVALGNAKKLAPTLPEVVALANRAER
                     RRPPEPPRAGWREALGDASTGVALVNSTAAAGHTDAAFLAASTMVALGTADDSLGALY
                     EQHRIRNLVIPSPLDHDHWSLLRHKDDTPELGGLLELVAPAVHALAPMRLADSDLDAG
                     QRVADSELPPVFSRMRNQLAEVLGIGGDAAVYARTELGFQIHVVASDPPVLVAGDEAL
                     TAPERPDLMFRLARAMTFLWPGRAVGASRPGRVLKAVVLSVVREAAGTDLGIDDPLAK
                     TADVAMIAVAPELREQARAAALRLLSRVGGGLNLSLWSRSLSRTADRTGMLLCGDVPA
                     AFTGAKEMGDLDRDLVEFAYSAAHVALRRQLGMSRG"
     gene            3195..3560
                     /locus_tag="VFV99_06270"
     CDS             3195..3560
                     /locus_tag="VFV99_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058944.1"
                     /translation="MDDRPLAKPEHLRDAPTSRGAWLAQRPAALASLVLGVLSFIVVA
                     ATQTQLWSTPDWRISVPGFALAAGASLMSLWRRERGHALWLAGVGFAGAALVLGWFFM
                     LALVIGATLLLMLILHTFM"
     gene            3568..4812
                     /locus_tag="VFV99_06275"
     CDS             3568..4812
                     /locus_tag="VFV99_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEX5058945.1"
                     /translation="MTREDSDLIYDWNSVEKVAPLSPKKRIQFLDETLRDGIQSPSVV
                     DPAIDDKLRIVDLANDLGIDTMDIGLPGAGKRAIEDVTVIAEHIRDAKLRVKASCAAR
                     THINDVQAIVDISQKVGIEIEVLCFIGSSPIRQYAEDWDISRMMKLSADAIDLGRKYN
                     LPVAYVTEDTTRSRPEVLTKLFTNAVEHGASRLIVCDTVGHVTPDGIRNLLKFTRNVL
                     DGMGRPDVGIDWHGHNDRGFGVVNSIFAIEYGADRIHGTALGIGERVGNAALDQILFN
                     LKLLGELPEHDLTKLVLWCKVVSQATRVPIHPQYPLVGSDAFRTATGVHAAAIIKAEK
                     KGHEWLADRIYSGVPAGMFGKEQEIEVGHYSGESNVVYWLKKRGYDPTKDLVAAVLAA
                     AKRGNRVLTDDEIVAVIKSQPA"
     gene            complement(4809..5279)
                     /locus_tag="VFV99_06280"
     CDS             complement(4809..5279)
                     /locus_tag="VFV99_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058946.1"
                     /translation="MQLTPELIEKMRAIGLRLDRAGTFWHEGSPITHPRLRVALLRWL
                     DVRDDGRDIVRLDEKRYAYVDVDDAHLRAQSARWDGGDRCMILWDDGQEEELDYASLR
                     QADDHALYARVRGGKLKGRIGGSAYHQVAEHVVEDDAGFVLAAAGKRWPIVNAA"
     gene            complement(5287..5472)
                     /locus_tag="VFV99_06285"
     CDS             complement(5287..5472)
                     /locus_tag="VFV99_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX5058947.1"
                     /translation="MSTNELSKETLLAELTAEHRRLDELLQQLERRRALTPTERNEIA
                     RLKKQKLLTKDRITRLS"
     gene            complement(5558..6238)
                     /gene="tsaB"
                     /locus_tag="VFV99_06290"
     CDS             complement(5558..6238)
                     /gene="tsaB"
                     /locus_tag="VFV99_06290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5058948.1"
                     /translation="MKVLGIDTASNIASVAVVQGQHVLAEQRSDTSGHRADLLVLVDA
                     VCTAAGVTARDLDGIAIGAGPGSFTGLRIGMATAKGIAFAAVRPLWAVSSLAALALPD
                     LVADPSAIVVGVLDARRGEVYAGAYAIGSSGPALVGEERVLPPGELAAFAPAGARFVG
                     DAAGAYPELAQLPGTWGQTPSGSAVAIIAQGGARVDALVSGAPTYIRPSEAEVKYPNG
                     VPGALRKR"
     gene            complement(6235..7884)
                     /gene="rseP"
                     /locus_tag="VFV99_06295"
     CDS             complement(6235..7884)
                     /gene="rseP"
                     /locus_tag="VFV99_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5058949.1"
                     /translation="MSVLYFLLLVGVLVVIHELGHFAAAKLLDVKVLRFSFGYGRPLV
                     RAKIGETEYQVAVFPLGGYVRILGIEGDSLAKGDAGRSFASRPLWQRVVIVLAGPAAN
                     LILPVVIYFVFFAGHTMLPAAQVGDVLEAGPAARAGIEPGDRVLEINGRTMHYWEDIE
                     QIVQSSPGKELQLRISRNGKEFERFVTPVQETVRQRDGRVKLQGRVGITHPPFVPLVG
                     VIDAESPAGRAGLVTGDLIISVDGQPVRNWNDVIHDLGRIARRSSIVYFRGTELPGVP
                     QIQLLSPGFADLVPETELDPTLKRHTYTGLEHAEMFVAHVDPGSPADSAGLKPGDLIV
                     ALDDKPVAHWLEFDQRLQAEPTKTYKLTWKRATTAGKTETLSAELTQVQRKELDDYGH
                     TVTRLVFGARNDVDRGTGAMTPIEGRFGYALSKALERTGDTISTMVSGFFQIVSGDKP
                     SDALGGPLMMYRVASVSGNQGWDAFLLMIALISINLGLINLLPIPMLDGGHLLVFAVE
                     GAMRKPLPAKARVRIQQAGLVVIGLIVLLAARNDVIRFFFS"
     gene            7931..9829
                     /locus_tag="VFV99_06300"
     CDS             7931..9829
                     /locus_tag="VFV99_06300"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX5058950.1"
                     /translation="MASKSQQDTPSVDVEELLDTVDSTLDRLKTLYEQYFLGIQKQAP
                     AFIHSDVERKLRDLTQINIRNTGLRYRLATLQQKFGSYNSYWRRTLRQIENGTYARNL
                     AKIGREAAKNGADIPEEILAAMPKRMREQVLRDRDQALALAKRRKQLEQAASEDGAAD
                     YEVLEDTAYDSEPEVTEATPIAAAPVVEKKTKTGAFVLDEDEPIDYNALFSAFDADDA
                     PKAGVRNKIPTVQPATRPQPLRMPKPSEQPRATPAAGTPPPASTAKPAVGTSPPPSAS
                     AKPAEPAKSAATVDAMINSIPRPVAQQPSGPTSSGVPRAIGQQPSQPTSSGVPRPVAK
                     QPSEPTSSGVPRPVGQQPSQPTATPPGGARLARVTPVQVAVPRSQDPTRQTGPVPRIS
                     DPSRQTGPVPAIDPSRQTGPMQAGDPSRQTGPVQRVDPSRQTGPTPAIPRPATGQVHT
                     IPRPAPSQQSRPIPVIPGSAQQTGPVPVESMNGPFPRTPASEKSGPTPTTQRQTGSVP
                     VPTTQRQTASVPVPTTQRQSGSVPVPTTQRQTGPIAKPTPSSTPKPPLKPPPGMSEAD
                     VNALHAKYVQAKQAIGEKVDAGSRDKLLKTINQTAPKIMEQYKASSVDFSVVVKDNQV
                     IIKAKPKT"
     gene            9921..11252
                     /gene="thrC"
                     /locus_tag="VFV99_06305"
     CDS             9921..11252
                     /gene="thrC"
                     /locus_tag="VFV99_06305"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5058951.1"
                     /translation="MPFTAQFRCIAGCSGTYPLEQVIYRCPQCGDLLEVVHDIAALRE
                     RSATQWKQLFGERWRSVEEPYGSGVWGKHEWVAPQLRASNIVSFLEGVTHLTRVPHYA
                     RELGLGDVRVKQCGTSHSGSFKDLGMTVLVSMVKQMITNGQPIRAIACASTGDTSAAL
                     AAYAAAAGIRAVVILPRGKISTAQLVQPLAMGALVLAIDTDFDGCMRLVQELASREGV
                     YLANSMNSLRLEGQKTVAIEIAQQLGWTVPDWVVIPGGNLGNVSALGAGFEMLHALGL
                     VDRRPRICVAQAAHAAPLATSYQHNWAELEPVKAKTTLASAIQIGNPVSFKKAVRALR
                     AFEGVVEAVSEAELADASARADRGGLFTCPHTGVALAALEKLAARGEVTKDQRVVVIS
                     TASGLKFADFKVGYHEGTLKDVPAPKYRNTPVELPEDYDAVRTALLRGLDK"
     gene            11249..11650
                     /gene="tsaA"
                     /locus_tag="VFV99_06310"
     CDS             11249..11650
                     /gene="tsaA"
                     /locus_tag="VFV99_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX5058952.1"
                     /translation="MTPVLTLIGVVESTLVNLDDSPRQGDEGAPDAWLVIAADFAAAL
                     DGLAAGDDILVFTWLDRARRDVLRVHPRGDRTRPEEGVFATRSPHRPNPIGLHRVTIV
                     AIEGTRVHVRPLEALDGTPIVDIKPVLDDER"
     gene            11669..11824
                     /locus_tag="VFV99_06315"
     CDS             11669..11824
                     /locus_tag="VFV99_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058953.1"
                     /translation="MRIAARWLGGGSIVLGTLAALLMVVSLYPGALERLSRADAQRAE
                     QFQSLAK"
     gene            11888..>12314
                     /locus_tag="VFV99_06320"
     CDS             11888..>12314
                     /locus_tag="VFV99_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5058954.1"
                     /translation="MARRPTEPLDEVTLRRAQRGDQRAWRDLIDRYQQPVHSLIWRLL
                     AGRARHRVEDLTQETFVRVLRALPGFDPSGPASLSTWILTIAARLALNELRRPEAAAI
                     EREPIGRDRTDADAERRRIGAAIAAGVAELSDDHRAVFVL"
BASE COUNT         2098 a         4135 c         4160 g         1921 t
ORIGIN      
        1 tgatgtattt gggccggatc gtggagctcg cggatgcgaa ggtcctgtat cgtgaaccga
       61 agcatcccta cacacaggca ctgctatcgg cggtgccgcg tgtcgctgta tccggggcga
      121 aatcaaagcg ggagcgcatc attttgcaag gtgatgtgcc gagcccgctg gcgccgccta
      181 ctgggtgcgc gttccatcca cgatgcccgg tcaaggacaa gcccgaagcc tgcttcaaag
      241 aggtgccgaa gctgcgtgtg ttatcgaacg gcagccgcgc cgcttgtcac gtcgccgaat
      301 aagttagtat cgaaagtcca tgcagtcgcg ctcgggggag cttgcgatgc ggccgctacc
      361 ggccgtgctc ctcgatcttc acgaggacat cgtcaccggt cgactcacgc tgcgtcgcgg
      421 caaggtcagc aagagcgtcg acctcatcaa cggcaacccg gtcagcaccg cgtcgacgcc
      481 gcgcgacgaa acgctcggtc attttctgac ctcgaccggc gtgatcactg aagaccagca
      541 ccgcgaggcc gtgcagcgcg cggcgaaggc ggacagcaag ctcggcgagg cgctcgtcgc
      601 gatgcaggtg ctgagcgtcg agcagctgat cgagcagctc ggcaagcagg cccgccacaa
      661 gctcgtgcaa gcgttgcgct ggccgcaggg tgcgtggcgg ttcgaggagg gtcgcgagcc
      721 gatcgagggc atgcagctgc gcatgatcga ggtcgtgctc ggcggcctgc gcgagaccgc
      781 ggtcgaagac ctcggccggc tgtcgcgact cgacggtatg tcgttcgagc tgaccgagcg
      841 tggcacgcgg ctcaagcacg agctgaagaa ggcgtttggc gagcgcgcgc ttgcggtgct
      901 cgcggcgggc gggccgatca ccgacatcga gaaagcgctc ggtgatcgcg tgcaggcgcg
      961 cgtcgcgctc gattcgatgc tgctctgcga cgcgatcgtg tcgaaggcga aggccgtcgg
     1021 gctcggcgcg cagctcgcga aggtgaaggt tgcacaggtg ccggcaccat cgccggcgat
     1081 cgagatcgag cccgacgagc cgccgccgga aggcgaggag ggcgaggagc tgttcgcgct
     1141 gctgttcgac gagatcgaag gcggcgagga cgatcacaag acgaaggacg gcgagaagcc
     1201 gctcgacttc gagctcgacg ccgtcgaggc cgtcgacgac cacaacgatt cgggcgtcgt
     1261 ctcgatggtc gatgtccagg agacgatcca gaagcgcgcg aaggcgcagg cggcgcggca
     1321 ggcgatcatc gccgagcacc agcgcatcca gggcgccgat cattacgctg ttctattgat
     1381 cgatcgcgac gcgacgaagg acgacatcga cggcggctac caggtcaagc tcacgctgct
     1441 gcagcgcaag accgacgagg tcactgaccc gcaggaccgc gcgaagctcg atcgcatcat
     1501 caccgcgtac aacaccgcgc gtgcgacgct gctcgatccg cagaagcgtg ctgcctacaa
     1561 tcgcgagctg gcaggtggcg agctcgtcga gggtccgccc gcgatcgata ccgagctgca
     1621 gttccgcatg gcggaggagc tgctcgcgaa gaagcagtgg gcgcaggcga tcggcatgct
     1681 gaagaccgtg atctcgcaat cgccgaacga ggccgattat cacgcggcgc tcggctgggc
     1741 gacgtggaat gcgggcaacg cgcaggcaaa cgccgccgac gaagcgcgca attacctgaa
     1801 ccacgcactg tcgatcaatc ccgatcaccc ggctgcgcac gactacaagg gccgcatcga
     1861 tgcgtcgttg cgcgtcgacg acgcgaacgc ggtgtttcac ctcgagcgcg cactcgatct
     1921 cgatccgcaa cgcaccgagg cgctcgcgac gctcgagaac ctgctcgtcg cgcgcggcga
     1981 gctgcgccgc ttcgagcgga tcatgaagcg cttgctgttc cgcttgcgcg gcaagaccca
     2041 cagcgtcgag gcgtcggcat gggcgcggct cacacggctc tatctcgatc atctcgacga
     2101 tcctggtgcc gccgcggtcg cgctcggcaa cgcgaagaag ctcgcgccga cgctcccaga
     2161 ggtcgttgcg ctcgcgaatc gcgccgagcg ccgccgacca cccgagccgc cgcgcgcggg
     2221 ctggcgcgaa gctctcggcg acgcgagcac cggcgtcgcg ctcgtcaaca gcacggccgc
     2281 cgccggtcac accgatgccg cgttcctcgc cgcgtcgacg atggtcgcgc tcggcaccgc
     2341 cgacgactcg ctcggtgcgc tctacgagca acatcgcatc cgcaacctcg tgatcccgag
     2401 cccgctcgat cacgatcact ggtcgctgtt acgccacaag gacgacacgc cggagctcgg
     2461 tgggctcctc gagctcgtcg cgcccgcagt tcacgcactc gcgccgatgc gactcgccga
     2521 cagcgacctc gacgcgggcc agcgtgtcgc cgactccgag ctgccgccgg tgttctcgcg
     2581 catgcgtaac cagctcgccg aagtgctcgg cattggcggc gacgccgcgg tgtacgcgcg
     2641 caccgagctc ggctttcaga tccacgtcgt cgcgagcgat ccgccggtgc tcgttgccgg
     2701 cgacgaggcg ctcacggcac ccgaacgccc ggacctcatg ttccggctcg cacgcgcgat
     2761 gacattcttg tggccgggcc gcgcggtcgg tgcgtcgcga ccaggtcgcg tgctcaaggc
     2821 agtcgtcctg tcggtcgtgc gcgaagcagc cggtaccgac ctcggcatcg acgatccgct
     2881 tgcgaagacc gcggacgtcg cgatgatcgc ggtagcgccg gagcttcgcg agcaggcacg
     2941 cgccgcggcg ctgcgtctgc tgtcgcgcgt gggcggtggc ctgaacctgt cgctgtggtc
     3001 gcgctcgttg tcgcgtaccg ccgaccggac cggcatgctg ctctgcggcg acgttcccgc
     3061 ggcattcacg ggcgctaagg aaatgggcga tctggaccgc gacctcgtcg agtttgccta
     3121 tagcgcggcg catgtcgcgc tgcgccggca actcggcatg tctagaggat agaacccgcg
     3181 ctataagacg gtcggtggac gaccgaccct tggcgaagcc cgagcatctg cgcgacgcac
     3241 cgacgtcgcg tggggcatgg ctcgcgcagc gcccagcggc gctcgcatcg ctggtgctcg
     3301 gcgtgctctc gttcattgtc gtcgccgcga cgcaaacgca gctatggtcg acaccagact
     3361 ggcgcatcag cgtgccgggc tttgcgctcg ctgcgggtgc gtcgttgatg tcgctgtggc
     3421 ggcgggagcg tggtcacgct ctgtggctcg caggtgtcgg ctttgccggc gccgcgctcg
     3481 tgctcggctg gttcttcatg ctcgcgctgg tgatcggcgc gacgttactc ttgatgctga
     3541 ttctccatac cttcatgtga ggctgccatg acccgcgaag attccgacct catctacgac
     3601 tggaactccg tcgagaaggt ggcaccgctc tctccgaaga agcgcatcca gttcctcgac
     3661 gaaacgctgc gcgatggcat tcagtcgccg tcggtcgtcg atccggcgat cgacgacaag
     3721 ctgcgcatcg tcgacctcgc gaacgatctc ggcatcgaca cgatggacat cggtctgccg
     3781 ggcgccggca agcgcgcgat cgaagatgtc accgtgatcg ccgagcacat tcgcgacgcg
     3841 aagctgcgcg tgaaggcgtc gtgcgcggcg cgcacgcaca tcaacgacgt ccaggcgatc
     3901 gtcgacatct cgcagaaggt cggcatcgag atcgaggtgc tctgcttcat cggcagctcg
     3961 ccgatccgcc agtacgccga ggactgggac atctcgcgga tgatgaagct gtcggcggac
     4021 gcgatcgatc tcggtcgcaa atacaacctg ccggtcgctt acgtcaccga ggacacgacg
     4081 cgctcgcgtc ccgaggtgct cacgaagctg ttcacgaacg cggtcgaaca cggcgcgtcg
     4141 cgcctgatcg tctgcgacac cgtcggccac gtcacgccgg atggaattcg gaatttgttg
     4201 aagttcacgc gcaacgtgct cgacggtatg ggccgcccgg acgtcggcat cgattggcac
     4261 ggtcacaacg accgcggctt cggcgtggtg aactcgatct tcgcgatcga gtacggcgcc
     4321 gatcgcatcc acggcaccgc gctcggcatc ggtgagcgcg tcggcaatgc ggcgctcgat
     4381 cagatcctgt tcaacctcaa gctgctcggc gagctgcccg agcatgatct gacgaagctc
     4441 gtgctgtggt gcaaggtcgt gtcgcaagcg acgcgcgtgc cgatccatcc gcagtatccg
     4501 ctcgtcggca gcgatgcgtt ccgcaccgcg accggcgtgc acgctgccgc gatcatcaag
     4561 gccgagaaga agggccacga gtggctcgcc gaccgcatct actccggcgt gcccgcgggc
     4621 atgttcggca aagagcagga gatcgaagtc ggtcactact cgggcgagtc gaacgtcgtc
     4681 tactggctca agaagcgcgg ctacgacccg acgaaggacc tcgtcgctgc cgtgctcgct
     4741 gccgcgaagc gcggcaaccg cgtgctcacc gacgacgaga tcgtcgccgt gatcaaatcg
     4801 cagccggcct aggccgcgtt gacgatcggc cagcgtttgc cggccgccgc gagcacgaag
     4861 ccggcgtcgt cctcgacgac gtgctcggcg acctggtggt acgccgatcc gccgatgcgc
     4921 cctttgagct tgccgccgcg taccctggcg tagagcgcgt ggtcgtcggc ctggcggagt
     4981 gatgcgtagt cgagctcttc ctcctggccg tcgtcccaca ggatcatgca gcgatcgccg
     5041 ccgtcccagc gtgccgattg cgcgcgcaga tgcgcgtcat cgacgtcgac atacgcgtag
     5101 cgcttttcgt cgagacgcac gatgtcgcgg ccatcgtcgc ggacatcgag ccagcgcagc
     5161 agcgctacac gaagtcgtgg gtgcgtgatg ggcgagcctt cgtgccagaa cgtgccggcg
     5221 cggtccaggc ggagcccgat cgcccgcatc ttttcgatca gctcgggcgt cagttgcata
     5281 cgcgcactat gacagacgcg tgatgcgatc cttggtgaga agcttttgct tcttcagccg
     5341 agcgatttcg tttctctcgg tcggggtcag tgcccgtcgt ctctcgagtt gttgcaggag
     5401 ctcatcgagg cggcggtgtt cggcagtcag ttcggcgagc agcgtttctt tcgaaagctc
     5461 gttggtgctc aagcggcgtc ctccgacact gtgaccatgt acctcgacga tacccccgga
     5521 tcgggagtgc ggcaacgcga gagtgcatga cgaacaatca ccgcttacgg agcgcgcccg
     5581 gtaccccgtt gggatatttg acctccgcct ccgacggccg gatgtacgtc ggcgcaccgc
     5641 tgacgagcgc gtcgacgcgt gcaccgcctt gcgcgatgat cgcgaccgcg ctgccgctcg
     5701 gtgtttgccc ccatgtgcca ggtagctgcg cgagctcggg atacgcgcca gctgcatcgc
     5761 cgacgaagcg cgcacccgcg ggcgcgaatg cggcaagctc gccgggtggc agcacgcgct
     5821 cctcgccgac gagcgcggga ccactgctgc cgatcgcgta cgcgcccgcg tagacctcac
     5881 cgcgtcgcgc gtcgagcacg ccgacgacga tggcgctcgg atccgcgacg agatcgggca
     5941 gcgcgagcgc cgcgagcgac gacaccgccc acagtggcct cactgccgcg aacgcgatgc
     6001 ccttcgcggt cgccatgccg atacgcaggc cggtgaacga tccgggaccg gcgccgatcg
     6061 cgatgccatc gaggtcgcgc gcggtgacgc cggcagcggt gcacacggcg tcgacgagaa
     6121 cgagcagatc ggcgcgatgg cctgacgtgt cgctgcgctg ctcggcgagc acgtgctggc
     6181 cctgcacgac cgcgacgctc gcgatgttgc tcgccgtatc gatgccgagg accttcacga
     6241 gaagaagaac cggatgacgt cgttgcgcgc tgcgagcagc acgatgaggc cgatcacgac
     6301 gaggcccgct tgctggatac gcacgcgcgc cttcgcaggt agcggcttgc gcatcgcgcc
     6361 ttcgaccgca aagacgagga ggtggccgcc gtcgagcatc ggaatcggca gcaggttgat
     6421 gaggccgaga ttgatgctga tcagcgcgat catcagcagg aacgcgtccc agccctgatt
     6481 gcccgagacc gatgcgactc gatacatcat gagcgggccg ccgagcgcat ccgacggctt
     6541 gtcgccgctc acgatctgga agaagccgct gaccatcgtg ctgatcgtgt cgccggtgcg
     6601 ctcgagcgcc ttcgacaacg cgtagccgaa gcggccttcg atcggcgtca tcgcgccggt
     6661 gccgcgatcg acgtcgtttc gcgcgccgaa cacgagccgc gtgacggtgt ggccgtagtc
     6721 gtcgagctcc ttgcgctgca cctgcgtgag ctcggcggac agcgtctcgg tcttgcccgc
     6781 ggtggtcgcg cgcttccacg tcagcttgta tgtcttcgtc ggctccgctt gcaggcgctg
     6841 atcgaattcg agccagtgcg cgaccggttt gtcgtcgagc gcgacgatca gatcgccggg
     6901 cttgaggccg gcgctgtcgg cgggtgagcc cggatcgacg tgcgcgacga acatctcggc
     6961 gtgctcgagg ccggtgtacg tgtgacgctt gagcgtcgga tcgagctcgg tctcggggac
     7021 gagatccgcg aagcccggcg acagcagttg gatctgcggc acgcccggca gctcggtacc
     7081 gcggaagtac acgatgctgc tccggcgtgc gatgcggccg agatcgtgga tgacgtcgtt
     7141 ccagttgcga accggctgac catcgacgct gatgatcaag tcgccggtca cgaggccggc
     7201 gcgacccgcg ggtgactccg catcgatcac accgacgagc ggcacgaacg gcggatgtgt
     7261 gatgccgacg cggccttgca gcttcacgcg gccatcgcgc tggcgcacgg tttcctggac
     7321 cggcgtgacg aagcgctcga actccttgcc gttgcgcgag atacggagct gaagctcctt
     7381 gcccggcgag ctctgcacga tctgctcgat gtcttcccag tagtgcatcg tgcggccgtt
     7441 gatctcgagc acgcgatcgc cgggctcgat gcccgcgcgc gcggcgggac ctgcttcgag
     7501 tacgtcgccg acctgcgcag cgggcagcat cgtgtggcct gcgaagaaca cgaagtagat
     7561 cacgaccggc agaatgagat ttgcggccgg gccggcgagc acgatcacga cgcgctgcca
     7621 cagcgggcgg cttgcgaagc tacggcctgc gtcgcccttt gccagcgagt cgccttcgat
     7681 gccgagaatg cgcacgtacc cgccgagcgg gaagacggcg acctggtact cggtctcacc
     7741 gatcttcgca cgtacgagcg ggcggccata cccgaacgag aaccgcaata ccttcacgtc
     7801 cagcaacttg gccgcggcga agtgcccgag ctcgtggatg accacgagga ccccgacgag
     7861 cagcagaaag tacaggacgg acatggaagc gcgtccgagg ctatagcaga gctggtatcc
     7921 tggggactag gtggcttcaa agtcccaaca ggacacgccg tctgtagacg tcgaagagct
     7981 cctcgacacg gtcgattcga cgctcgaccg gctcaagacg ctctacgaac aatactttct
     8041 cgggatccag aagcaggcgc cggcgttcat ccacagcgat gtcgagcgca agctgcgcga
     8101 cctgacccag atcaacatcc gcaacacggg cctgcgctac cggctcgcga cactgcagca
     8161 gaagtttggc tcgtacaact cgtactggcg ccgcacgctg cgccagatcg agaacggcac
     8221 gtacgcgcgc aacctcgcca agatcgggcg cgaggctgcg aagaacggcg ccgacatccc
     8281 cgaagaaatt ctcgccgcga tgccaaagcg catgcgcgag caagttctcc gcgatcgcga
     8341 tcaagcactc gcgctcgcga agcgccgcaa gcagctggag caggccgcga gcgaggacgg
     8401 cgcggccgac tacgaggtgc tcgaggacac cgcctacgac agcgagcccg aggttaccga
     8461 ggcgacgccg attgcggctg cgcccgtcgt cgaaaagaag acgaagaccg gcgccttcgt
     8521 gctcgatgag gatgagccga tcgactacaa cgcgctgttc tccgcgttcg acgctgacga
     8581 cgcgccgaag gccggcgtgc gcaacaagat cccgacggtt cagccggcca cgcggccaca
     8641 accactgcgc atgccgaagc cgagcgaaca gccacgcgcg acgcccgccg cgggaacgcc
     8701 gccgcctgcg agcacagcga agcccgccgt cggcacgtcg ccgccgccgt cggcgagcgc
     8761 aaagcccgcc gagcccgcga agtctgcggc aaccgtagac gcgatgatca attcgattcc
     8821 gcgcccggtc gctcagcagc cgagcgggcc gacgtcgagc ggtgtgccgc gtgcgatcgg
     8881 tcagcagccg agccaaccga cgtcgagcgg cgtgccgcgt ccggtcgcta agcagccgag
     8941 cgagccgacg tcgagtggcg tgccgcgccc cgtcggtcag caaccaagtc agccgaccgc
     9001 gaccccacct ggcggagcca ggctcgcgcg cgtcacgccg gtgcaggtag cggtgccacg
     9061 ctcgcaggat ccgacgcggc agaccggtcc cgtcccgcga atttcggatc cgtcacggca
     9121 gaccggtccc gtgccggcaa tcgatccatc gcgacagacg ggccctatgc aggcgggtga
     9181 tccgtcgcgg cagacggggc cggtgcagcg ggtcgatccg tcgcggcaga ccgggccgac
     9241 gcccgccatc ccacgcccgg ccactggcca agtgcacacg attccgcgtc cagcgccgtc
     9301 gcaacagtcg cggccgattc cggtgatccc gggctcggcg cagcagaccg ggccggtgcc
     9361 ggtcgagtcg atgaacggcc cgttcccgcg cacgcccgcc tccgaaaaat caggaccgac
     9421 gccgacgaca cagcgtcaga ccggttcggt tccggtgccg acgacgcagc gccagaccgc
     9481 ctcggtgccg gtgccgacga cgcagcggca gagcggttcg gtgccggttc cgacgacgca
     9541 gcgccaaacc gggccgatcg cgaagccgac gccatcgtcg acgccgaagc cgccactcaa
     9601 accgccaccc ggcatgagcg aagccgacgt gaacgcgctg cacgcgaagt acgtgcaggc
     9661 gaagcaggcg atcggcgaga aggtcgacgc cggcagccgc gacaagctgc tcaagacgat
     9721 caatcagact gcgccgaaga tcatggagca gtacaaggcg tcgagtgtcg acttctcggt
     9781 cgtcgtcaaa gacaaccagg tcatcatcaa agcgaagccg aagacgtaat cctgctccgc
     9841 ccggccactg ggccacgccg gcggtagccg agcgcgcgtc atcgccggcc gttcgaccga
     9901 gcccggggta cagtcgcgtc atgccgttca cggcgcagtt tcgctgcatc gcaggttgct
     9961 cggggaccta tccgctcgag caggtgatct atcgctgccc gcagtgcggc gacttgctcg
    10021 aagtcgtgca cgacatcgcg gcgctgcgcg agcgctcggc gacgcagtgg aagcaactgt
    10081 tcggggagcg ctggcgttcc gtcgaggagc cgtacggctc gggcgtgtgg ggcaagcacg
    10141 agtgggtcgc gccgcagcta cgggcgtcga acatcgtgtc gttcctcgaa ggcgtcacgc
    10201 acctgacgcg cgtgccgcac tacgcgcgcg agctcgggct cggtgacgtg cgcgtcaagc
    10261 aatgcggcac gtcgcactcg ggttcgttca aggacctcgg catgaccgtg ctcgtgtcga
    10321 tggtcaagca gatgatcacg aacggccagc cgatccgcgc gatcgcgtgc gcgtcgacgg
    10381 gcgatacatc cgctgcgctc gccgcctacg ccgctgccgc cggcattcgg gcggtcgtga
    10441 tcctgccgcg ggggaagatc tcgacggcgc agctcgtcca gccgctcgcg atgggcgcgc
    10501 tcgtgctcgc gatcgacacc gacttcgacg gctgcatgcg gctcgtgcaa gagctcgcgt
    10561 cgcgcgaagg cgtctatctc gcgaactcga tgaactcgct gcgcctcgaa ggccagaaga
    10621 ccgtcgcgat cgagatcgcg cagcagctcg gctggacggt gcccgactgg gtcgtgattc
    10681 cgggcggcaa cctcggcaac gtgtccgcgc tcggtgccgg cttcgagatg ctgcacgcgc
    10741 tcggcctcgt cgatcgccgg ccgcggatct gcgtcgcgca agccgcacac gccgcgccgc
    10801 tcgccacgag ctatcagcac aactgggccg agctcgaacc ggtcaaagcg aagaccacgc
    10861 tggcgtccgc gatccagatc gggaacccgg tcagcttcaa gaaggccgtg cgtgcgctgc
    10921 gcgcgttcga gggcgtcgtc gaagccgtca gcgaagccga gctcgcggat gcatccgcac
    10981 gcgccgatcg cggtggcctg ttcacgtgcc cgcacaccgg tgtcgcgctc gccgcgctcg
    11041 agaagctcgc ggcacgcggc gaggtcacca aggatcagcg cgtcgtcgtg atctcgacgg
    11101 cgagcggcct caagttcgcc gacttcaagg tcggctatca cgagggcacg ctgaaggacg
    11161 tgccggcgcc gaagtaccgc aacacgccgg tcgagctgcc cgaggactac gacgccgtcc
    11221 gcacggcgct gcttcgcggc ctcgacaaat gacgccggta ctgacgctga tcggcgtggt
    11281 cgagtcgacg ctcgtcaatc tcgacgattc gccgcgccaa ggtgacgaag gtgcgcccga
    11341 tgcgtggctc gtgatcgctg ctgacttcgc ggccgcgctc gacggtctcg ccgccggcga
    11401 cgacattctg gtgttcacgt ggctcgatcg tgcgcggcgc gacgtgctgc gcgtgcatcc
    11461 gcgtggcgat cgcacgcggc cggaggaggg agtgttcgcg acgcgctcgc cacatcggcc
    11521 aaatccgatc ggcttgcacc gcgtgacgat cgtggcgatc gaaggtacgc gcgtacatgt
    11581 gcgtccgctc gaagcactcg acggtacgcc gatcgtcgac atcaagccgg tactcgacga
    11641 cgaacgctga cccgcgtacg atcggttcat gaggatcgcg gcgcgttggc tcgggggcgg
    11701 ttcgatcgtg ctcggaaccc tcgccgccct tctgatggtg gtctcgctgt atcccggggc
    11761 gctcgaacgg ctgagccggg ccgatgccca gcgggccgaa caattccagt cgcttgcaaa
    11821 atagtcccgc ggtccgcgga acatttgccg ccgtcgcggg tgatacccga cacggatgca
    11881 ggcggccatg gcgcggcgac cgaccgagcc gctcgacgaa gtcacgcttc gtcgggcgca
    11941 gcgcggcgat cagcgcgcgt ggcgtgatct gatcgatcga tatcagcaac cggtgcactc
    12001 gctgatctgg cgactgctcg ccgggcgcgc acgccatcgc gtcgaagacc tcacgcagga
    12061 gacgttcgtg cgcgtgctgc gcgcgctgcc cgggttcgat ccatcggggc cggcgagcct
    12121 gtcgacgtgg atcctgacga tcgcggcgcg cctcgcgctc aacgagctgc gccgtcccga
    12181 ggccgctgcg atcgagcgcg agccgatcgg tcgcgatcgt acagatgcgg acgccgagcg
    12241 gcggcgcatc ggggccgcga tcgcagccgg cgttgccgag ctctcggatg accatcgcgc
    12301 cgtgttcgtg ctgc
//
LOCUS       DASUFX010000158         6438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1969739, whole genome shotgun sequence.
ACCESSION   DASUFX010000158 DASUFX010000000
VERSION     DASUFX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969739"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFV99_06325"
     CDS             complement(<1..935)
                     /locus_tag="VFV99_06325"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5058955.1"
                     /translation="MAVATYPGTVSPLRGLDPFGEAERDVWQGRESERDELARMVTAD
                     GFRAGLLFGEQGVGKTSLVRAGLIPHLRDHGVVALACEDLSQPAQSFAIGLSTFGIQP
                     NSGEAPIPFMIRAVSNAVQGQQFVFIVDDVDLAVHDDRITSELSEIFAKVVSRSAGRA
                     RFLFVCASDRLNALGALERRTGSLFPPSNRYELPRVQAAAAAQIFDRVLSLSGVAADP
                     QLAEAVVRGLDGGQGVIAADLQIAAMAMRDLRITSVAALQKIGGASELEAAWLHDACR
                     ATGNERSALRLCAEMATGSHGPIGADEIIRRTNIEP"
     gene            997..2457
                     /locus_tag="VFV99_06330"
     CDS             997..2457
                     /locus_tag="VFV99_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX5058956.1"
                     /translation="MGRSSAVLWFGVVLAVGATDAGAGGAQTSITVFATQPAAISPYG
                     GISYGGYGSPTGAMITERRELDVTSGGEVRISGIASTVDPATVQLRSLTEPGGLSVSE
                     QRFVPGATTPDEILARHVGDTVTIVTPKGDVNGVLRSIDSQTLVLEVGTGDQRRLQMM
                     RRDGYVQDIKLPAGKQVDKPSLVWRLATKKPGKHTVEVTYRADGLSWAADYLAIFDET
                     AKTIDFSAWATLRNSTGTSFDGAELTLVSGGSMLPSVSVNPYQMYGQAPVRQSTPPLR
                     FTVPSLIHVGNGESVQVELMPPKIGAKARSIVTYEAVQDLSINYQQYPANDCTMLTQT
                     TPGGGRAEVAIEVDIPGAKQLPDGRVRVFKRAAKMPDRLEVLTEEQLRASAGIARVRL
                     ATHTELVGERKTTCTVDERARTLTEKVEVKVENKGKQSVDAVVREFLWRYPVWKIDPA
                     DESIKGTRGGAQTQEYRVAVPAGAKKSLTYTVVYQW"
     gene            2543..3313
                     /locus_tag="VFV99_06335"
     CDS             2543..3313
                     /locus_tag="VFV99_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058957.1"
                     /translation="MRVAAIMVLLCACGDDGASNAVDASLADAPPDAQVKKAGLVAIV
                     EDKAMNQTIVLGNFFVDHSTVVASRDDGSCHIEQRNTVMELPINGGTITIMGGGAGTV
                     SVPFDPMVGGYVAQGTGLSYGPGDQLTISTSGAMVPPLSGQLVFPVRVTVTSGTPTVL
                     HKSGFTATWDPTAGPVTIGITQYPSGAPELRITCAFDGTAGTGTIPAAALSDVLTTTS
                     VGIGVSMQTRLMVMAGDYPVNLFAIYGALDANSVPVQP"
     gene            3364..4737
                     /locus_tag="VFV99_06340"
     CDS             3364..4737
                     /locus_tag="VFV99_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058958.1"
                     /translation="MRERFAVQLSAALVLIGCGARQPASKPPAVVGDDVTLYRDWSVV
                     RQRIDLDVPATATTLSVIVAAGIAADQVMVIDRGGLTINALHAQTDPNADDANNEEPL
                     DERVSEAPAIDEPPPPEPEDDGEEPPKPVKAVAEISEPRHDVKPTELRLDVTAPHTGK
                     YSIVIGYITNRLHWDVAYTMAATPKRDRGVLRGALAIRNTTGVTLHAATARLIDAELG
                     AWRGKSAEQLATSLVGGTPASTAPAAARELGSIDVGAGETRVELIGASTRKMSSVLVY
                     DPIGTKLDNPGAAPLRDARLGLDPPPSTRVTESFEVARDRAATVGLPAGPVRLLERRA
                     NGALVVLGESRLFEATARVANLDTISVGTADRVTGTRERHELTIDDENRRIVEEFVIT
                     LDNQREAPVSVLVREHLYRGQNWTLAYHSAVVASKEGPQQIALRAEVPAKQKLKILYV
                     VVYTWGQ"
     gene            4734..5459
                     /locus_tag="VFV99_06345"
     CDS             4734..5459
                     /locus_tag="VFV99_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5058959.1"
                     /translation="MIASVSELTIQYGKRVAVDGLDLTLDGGEIALLLGPNGAGKSTT
                     LSAMAGAVLPSRGTIKVDGENLATAPRSARAKVGFADQPPALYEFFTVEEHVGFVAEA
                     RGAAATATAAVLADLSLSKIAQRPCRELSFGMRQRVGLAAALVGPVKLVLLDETLNGL
                     DPHASRAARATLRAAADKGAAILMSTHLLGVAERMCDRILVMNKGKLVADKRGDEVKH
                     LLAQGPNAVEELYVSLIADEGPT"
     gene            5755..>6438
                     /locus_tag="VFV99_06350"
     CDS             5755..>6438
                     /locus_tag="VFV99_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058960.1"
                     /translation="MTSKQFLHWTRLYEKRSGHALRGWIVALVAGVAFAGFVYWRADV
                     AGPIAASHAWLAGALAAFALAFMRVPFHVYWRQDAALLAQLPIGGGALFDAAWLRCIR
                     AALATCITVAIGALPLALLDRELVSFATREFLPTPIAGPSAAGLTPIEFALRHLALTG
                     VLGLLAACLMPAVATWAASLVTHGRDALEFATKLAGAPVRAEAKATAEQITYTDRGST
                     SAILGAVPGF"
BASE COUNT         1092 a         2243 c         2116 g          987 t
ORIGIN      
        1 ggctcgatgt tcgtacggcg aatgatctcg tcagccccga tcggcccgtg cgagccggtc
       61 gccatctcgg cgcacagccg gagtgccgag cgctcgttgc cggtggcgcg acacgcgtcg
      121 tgcagccatg ccgcttcgag ctcgctcgcg ccgccgatct tctgcaatgc cgcaaccgac
      181 gtgatccgca ggtcgcgcat cgccatcgct gcgatctgga gatccgcagc gatcacgccc
      241 tgaccaccat cgagcccgcg cacgaccgct tccgcgagct gcggatcggc cgcgacgccc
      301 gacagcgaca acacgcgatc gaagatctgc gccgccgcag cggcctggac acgcggaagc
      361 tcgtaccggt tgctcggcgg aaacagcgag ccggtccgcc gctcgagcgc gccgagcgcg
      421 ttcaatcgat cgctcgcgca cacgaacaag aaccgcgcgc ggccagcgga gcggctcacc
      481 actttcgcga aaatctcact cagctccgac gtgatccggt cgtcgtggac cgcgagatcg
      541 acgtcatcga cgatgaacac gaactgctgg ccctgcaccg cgttcgagac cgcacggatc
      601 atgaacggaa tcggcgcctc gcccgagttc ggctgaatcc caaacgtcga gaggcctatc
      661 gcaaagctct gcgcgggctg gctcaagtcc tcgcacgcaa gggcaacgac gccgtggtca
      721 cgcaaatgcg ggatcaatcc cgcgcgcacc aacgaggtct tgccgacacc ttgctcgccg
      781 aacagcaggc ccgcgcggaa gccgtccgcc gtaaccatgc gagccaactc atctcgttcg
      841 ctctcgcggc cctgccacac atcacgctcg gcttcgccga acggatcgag gccccgcaag
      901 ggcgacaccg ttcccggata agtcgcgacc gccacggccg cactatacac aaggatgtac
      961 cggcgctggc gccgaccatc atctatgctt cgtcgcatgg ggcgctcgtc cgcagttctc
     1021 tggttcggcg tcgtgctcgc cgtcggtgca accgatgccg gtgccggcgg tgcgcagaca
     1081 tcgatcacgg tgttcgcgac gcagcccgcg gcaatttcgc cgtacggcgg catttcatac
     1141 ggcggctacg gctcgccgac cggtgcgatg attaccgagc ggcgcgagct cgacgtcacg
     1201 agtggcggcg aggtccgcat cagcggcatc gcgtcgacgg tcgatccggc caccgtgcag
     1261 ctacgtagtc ttaccgaacc gggcggcctc tccgtcagcg aacagcgttt cgtgcccggc
     1321 gcgacgacgc ccgatgaaat cctcgcgcgc cacgtcggcg acaccgtcac gatcgtgacg
     1381 cccaaaggcg acgtcaacgg cgtgctgcgc tcgatcgact cgcagacgct cgtcctcgag
     1441 gtcggcaccg gcgatcaacg ccgcctccag atgatgcgcc gtgacggcta cgtccaggac
     1501 atcaagctgc cggccggcaa gcaagtcgac aagccgagcc tcgtctggcg cctcgcgacg
     1561 aagaagcccg gcaagcacac cgtcgaggtc acgtaccgcg ccgacggcct gtcgtgggcg
     1621 gccgactatc tcgcgatctt cgacgagacc gcgaagacga tcgacttctc cgcgtgggcg
     1681 acgctgcgca actccacggg aacgagcttc gacggcgcgg agctcacgct cgtcagcggc
     1741 ggcagcatgt tgccgtcggt ctcggtgaat ccgtaccaga tgtatgggca agcaccggtg
     1801 cgacagtcga cgccgcccct gcgcttcacg gtgccgagcc tcatccacgt cggcaacggc
     1861 gagtcagtgc aagtcgagct gatgccgccg aagatcggcg cgaaggcgcg ctcgatcgtc
     1921 acctacgagg cggttcagga tctatcgatc aactatcagc agtatcccgc gaacgactgc
     1981 acgatgctga cgcagaccac acccggtggc ggccgcgccg aggtcgcgat cgaggtcgac
     2041 attcccggcg cgaagcagct acccgacggg cgcgtccgcg tgttcaagcg cgcggcgaag
     2101 atgcccgatc gactcgaagt gctcaccgaa gaacagctgc gcgcgagcgc cggcatcgcg
     2161 cgcgtgcgcc tcgcgacgca caccgagctc gtcggcgagc gcaagacgac gtgcaccgtc
     2221 gacgaacgcg cgcgcacgct gaccgagaag gtcgaagtca aggtcgagaa caagggcaag
     2281 cagtccgtcg acgcggtcgt ccgcgagttc ttgtggcgct acccggtgtg gaagatcgat
     2341 cccgccgacg agagcatcaa gggcacgcgc ggtggcgcgc agacccagga gtatcgagtc
     2401 gcggtacccg cgggcgcgaa gaagtcgctg acgtacaccg tcgtctatca gtggtgagcg
     2461 actagagccg cgtacgtggc tcgtcgcaca ccgccgtatt tggttgcggc ggcagcgccg
     2521 ctttcgacga tatgatcgag ctatgcgagt ggccgcgatc atggttctcc tgtgtgcctg
     2581 tggcgacgat ggtgcaagca acgcggtaga tgcatcgctc gccgatgcac cgcctgacgc
     2641 gcaggtgaag aaggcaggcc tcgtcgccat cgtcgaagac aaggcgatga accaaacgat
     2701 cgtgctcgga aacttcttcg tcgatcacag cacggtcgtc gcgagtcgcg atgacggctc
     2761 gtgtcatatc gaacagcgca acacggtgat ggagctgccc atcaacggcg gcacgatcac
     2821 gatcatgggt ggcggcgcgg gcacggtatc cgtcccattc gatccgatgg tcggcggcta
     2881 cgtcgcgcag ggcactggcc tctcgtacgg gcccggcgac cagctcacga tctcgacgtc
     2941 gggggcgatg gtacctccgt tgtccgggca gctcgtattc cccgtgcgag tgacggtgac
     3001 gagcgggaca cccaccgtgc tccacaagtc cgggttcacg gcgacgtggg acccgacggc
     3061 gggccccgtc acgatcggca tcacgcaata cccgagcggc gcgcccgagt tgaggatcac
     3121 ctgtgcgttc gacggtaccg ctggcaccgg aaccatcccg gcagccgcac tgagcgacgt
     3181 gctgacgacc acctctgtcg gcatcggagt ctcgatgcag acccggctga tggtgatggc
     3241 cggcgattac cccgtgaatc tgtttgccat ctacggtgcg ctggacgcca acagcgttcc
     3301 ggttcagccc tgaccgttcg ctcgatagca aaccgcgacg ttcgccgtca tactttgaag
     3361 acgttgcgcg agcgattcgc cgtccagttg tccgcagcac tcgtgctgat cggctgtggc
     3421 gcgcggcagc ctgcatcgaa gccccctgct gtggtgggcg atgacgtcac gctgtatcgc
     3481 gattggtcgg tggtgcggca gcgcatcgac ctcgacgtgc cggcgaccgc gacgacactc
     3541 tcggtaatag tcgccgctgg aatcgccgcc gatcaagtca tggtgatcga tcgcggcggc
     3601 ctcacgatca acgcgctgca cgcgcagacc gatccgaacg ccgacgatgc gaacaacgaa
     3661 gagccgctcg acgagcgggt cagcgaggcg ccggcgatcg acgagccacc accgccggaa
     3721 cccgaggatg atggcgaaga gccgccgaag ccggtgaagg cagtcgccga gatttccgag
     3781 ccccgtcacg acgtcaagcc gaccgagctg cgcctcgatg tcaccgcgcc gcataccggc
     3841 aagtactcga tcgtgatcgg ctacatcacg aaccggctgc actgggacgt cgcgtacacg
     3901 atggcggcga caccgaagcg cgaccgcggc gtgctgcgcg gtgcactcgc gattcgcaac
     3961 acgaccggcg tcacgcttca tgccgcgacg gcccgcctga tcgacgcgga gctcggcgcg
     4021 tggcgcggca agtcggccga gcagctcgcg acgtcgctcg tcggcggcac accggcatcg
     4081 accgcacccg cagcggcgcg cgagctcggc tcgatcgacg tcggcgccgg cgagacccgc
     4141 gtcgagctga taggcgcgtc gacgcgcaag atgagctcgg tgctcgtcta cgatccgatc
     4201 ggtacgaagc tcgacaatcc gggcgccgcg ccgcttcgcg atgcgcgcct cggcctcgat
     4261 ccaccgccgt cgacgcgcgt caccgagagc ttcgaggttg cgcgcgaccg cgccgccacc
     4321 gtcgggttgc cggcgggtcc ggtccgcctg ctcgagcgcc gcgcgaacgg cgcactcgtc
     4381 gtgctcggcg agtcgcgcct gttcgaagcg accgcgcgcg tcgcgaacct cgacacgatc
     4441 tcggtcggca cggccgatcg cgtgaccggc acgcgcgaac gccacgagct caccatcgac
     4501 gacgagaacc gccgcatcgt cgaggaattc gtgatcacgc tcgacaacca gcgcgaagcg
     4561 ccggtctcgg tgctggtacg tgaacatctg taccgcggcc agaactggac gctcgcctat
     4621 cacagcgccg tggtggcctc caaagaggga ccgcaacaga tcgcgctgcg tgccgaagtt
     4681 ccggccaagc agaagctcaa gatcctctac gtcgtggtgt acacctgggg tcagtgatcg
     4741 ccagcgtctc cgagctcacg atccagtacg gcaagcgcgt cgcggtcgac ggtctcgacc
     4801 tcacgctcga cggcggcgag atcgcgctgc tgctgggacc gaacggtgcg ggcaagagca
     4861 cgacgctttc cgcgatggcg ggcgccgtgt tgccgagccg aggcacgatc aaggtcgacg
     4921 gcgagaacct cgcgacggca ccacgctcgg cgcgcgcgaa ggtcggcttc gccgatcagc
     4981 cgccggcact ctacgagttc ttcaccgtcg aggagcacgt cggcttcgtc gccgaggcgc
     5041 gcggtgccgc tgccactgcg accgcggcgg tgctcgccga tctctcgctc tcgaagatcg
     5101 cgcagcggcc gtgccgcgag ctgtcattcg gcatgcgcca gcgcgtgggc ctcgccgcgg
     5161 cgctcgtcgg tccggtcaag ctcgtgctgc tcgacgagac gctgaacggg ctcgacccgc
     5221 acgcatcgcg ggccgcgcgc gcgactctgc gtgctgccgc cgacaagggc gcagcgatct
     5281 tgatgtcgac gcacttgctc ggtgtcgccg agcgcatgtg cgatcgcatc ctcgtcatga
     5341 acaaggggaa gctcgtcgcc gacaagcgcg gtgacgaggt gaaacacttg ctcgcgcaag
     5401 gcccgaacgc cgtcgaggag ctctacgtct cgctgatcgc cgacgagggc ccgacgtgac
     5461 gtcgcagatc cgatcaaccg ccaagacgtc gacggacgcc aagccggctc ccggcaatgc
     5521 acagagatca accgccaaga cgccggcaga cgccaagcgc agaagggttt cgatccagag
     5581 cgagctccgc gctggttcgc gatcgtcgtg ctcgagcgcg gcgcatgttc gtacagagcg
     5641 ccgtcctgaa gaagccctcc ggccttggcg tctgcggcgt cttggcggtt caactcgaga
     5701 gcgccggcct tggcgtttgt ggcggcttgg cggttcaact ctccgaggaa cgccgtgacg
     5761 agcaagcagt tcctccactg gacccgtctc tacgagaagc gctccggtca cgcgctgcgc
     5821 ggctggatcg tcgcgctcgt cgccggcgtc gcgttcgccg gcttcgtgta ctggcgcgcc
     5881 gacgtcgcgg gtccgatcgc ggccagccat gcgtggctcg ccggcgcact cgccgcgttc
     5941 gcgctcgcgt tcatgcgcgt gccgtttcac gtctactggc gccaggatgc ggcgctgctc
     6001 gcgcagctcc cgatcggcgg cggcgcgttg ttcgacgctg cctggctgcg ctgcatccgc
     6061 gccgcgctcg ccacctgcat cacggtcgcg atcggcgcgc taccgctcgc gctgctcgat
     6121 cgcgagctcg tctcgttcgc gacgcgggag tttctgccga cgccgatcgc gggcccgagc
     6181 gccgcagggc tgacgccgat cgaattcgcg cttcgccacc tcgccctcac cggtgtgctc
     6241 ggcctgttgg ccgcgtgcct catgccggcc gtggccacgt gggcggcgtc gctcgtgacg
     6301 cacggtcgcg acgcactcga attcgcgacc aagctcgccg gcgcgcccgt gcgcgcggag
     6361 gcgaaggcga ccgccgagca gatcacctac accgatcgcg ggagcacgag cgcgatcctc
     6421 ggcgcggtcc ccggcttc
//
LOCUS       DASUFX010000159         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1831094, whole genome shotgun sequence.
ACCESSION   DASUFX010000159 DASUFX010000000
VERSION     DASUFX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831094"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..>2220)
                     /locus_tag="VFV99_06355"
     CDS             complement(219..>2220)
                     /locus_tag="VFV99_06355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HEX5058961.1"
                     /translation="IYDAGTDNVRGPFYVMRKVTETSLEMILHQRKTGEGNPHDYTLN
                     RLLRYFLQVCNAVDYAHHRGVIHCDIKPANILLGDYGEVLLVDWGLAQSRNHPLGVRG
                     GTLGYMAPEQMDPKIERLDWRTDVFALGAIIYEILCGLPAFKDARNSDVTAVGKDPAR
                     IYKTPELPSLKTAVEIPREVEEICMQAIAVDREQRLETVRELANAIEEWIEGSKQKER
                     QRIEADKSADTGDELAERYHEFVESRPEKLTVFRALRADVLPFAPLDDKQDLWDAEDI
                     VRVTDALQVRTFHSAVSAYERALDAVPGHTRARRGLARLYWAELQRARHRGESLDQVA
                     YEQLLREVDDGTFVRELQRDGLLELTIGAHAGRVTLAKLVERQRRLVEGSSEVIEERS
                     SAHRSLAAGRYVVTSTIGDGPGTVSWPVSIEAGQLCRISVEPPEVGRIGDNEVLIPAG
                     PARLGGDPFASESDEVVIADVPAFVLQRFPVTFLQWNQFLDDMRKHGLAFASKHVPKL
                     RVGDQGNPDVPVFGISAESAEAYAEWLSKKDGRTWRLPTEIEWEKAARGTDGRIYPWG
                     DHFDALFCKMRDSRPGTPTAEPIGTFEWDVSPYGVRDLAGGVADWCIPDHRRTAPREP
                     REVVSRGGAWCDWAIDCRLASRRRYLATEHSARVGVRLARDP"
BASE COUNT          378 a          771 c          682 g          389 t
ORIGIN      
        1 gtcgacgagt tgaagtgctc gatcttctcg aagtgcatct cgatcgagcc cttcttctcg
       61 ctcactgaag cgagaagctt ctcgaaccat ggcttcaaga tttcgggcgg gttgcgctcg
      121 ttgctcttgc cacgccacac gcaactaaat gtggcgtccg gtgacatcgt cacctcgatt
      181 tgtaggtcgc ctgaggtcag attctccata gctctcctct aaggatcacg cgccaaccgc
      241 acgcccacgc gtgccgaatg ctctgtcgcc agataacgcc ggcgggaagc caaccggcaa
      301 tcgatcgccc aatcacacca cgcaccacca cgcgacacca cttcgcgcgg ttcgcgcgga
      361 gcggtgcggc gatgatcagg aatgcaccaa tcggcgacgc cgccggcgag gtcgcgcacg
      421 ccgtagggcg acacatccca ctcgaacgta ccgatcggct cggcggtcgg cgtgcccggt
      481 cggctgtcgc gcatcttgca gaacaacgcg tcgaagtgat cgccccacgg atagatgcgg
      541 ccgtcggtgc cgcgcgccgc cttctcccac tcgatctcgg tcggcagccg ccacgtgcgc
      601 ccatccttct tcgagagcca ctccgcgtac gcctccgccg attcggcgct gatgccgaac
      661 accggcacgt caggattgcc ctgatctcca acgcgtagct tcgggacatg cttcgaggcg
      721 aacgccaagc cgtgcttgcg catgtcgtcg aggaattgat tccactgcag gaacgtgacc
      781 gggaaccgtt gcagcacgaa cgcaggcacg tcggcgatca caacctcgtc gctctcgctc
      841 gcgaacggat cgccgccgag tcgcgccggg cccgccggaa tcagtacctc gttgtcgccg
      901 atccggccga cctcgggcgg ctcgaccgag atccggcaca gctgccccgc ctcgatgctg
      961 accggccacg acaccgtgcc cggaccgtcg ccgatcgtcg acgtcacgac gtagcggccg
     1021 gcggcaagtg accggtgtgc actcgagcgc tcctcgatca cctcggacga gccctcgaca
     1081 agccggcgct gacgctcgac gagcttcgcg agcgtcacgc gaccggcatg cgcgccgatc
     1141 gtcaactcga gcaaaccgtc gcgctggagc tcgcgcacga acgtgccatc atcgacctcg
     1201 cggagcagct gctcgtacgc gacctgatcg agcgactcgc cgcgatgtcg tgcgcgctgg
     1261 agctccgccc agtacaaacg tgcgaggccg cgccgcgcac gcgtatggcc tggcactgcg
     1321 tcgagcgcac gctcgtaggc agagacggcc gagtggaacg tgcggacctg cagcgcgtcg
     1381 gtgacccgca cgatgtcctc ggcatcccac agatcctgtt tgtcatcgag cggcgcaaac
     1441 ggcagcacgt ccgcgcgcag cgcacgaaac acggtgagct tctccggccg cgactcgacg
     1501 aactcgtgat agcgctccgc cagctcgtca ccggtgtcgg cactcttgtc ggcctcgatg
     1561 cgctgccgct ccttctgctt gctaccttcg atccattctt cgatcgcgtt cgcgagctcg
     1621 cgcacggtct cgaggcgctg ctcgcggtcg accgcgatcg cctgcatgca aatctcctcg
     1681 acctcgcggg gaatctcgac cgcggtcttc agcgagggca gctccggtgt cttgtagatg
     1741 cgcgccgggt ccttgccgac cgcggtcacg tcggagttgc gcgcgtcctt gaacgccggc
     1801 aggccgcaca ggatctcgta gatgatcgcg ccgagcgcga acacgtcggt gcgccagtcg
     1861 aggcgctcga tcttcggatc catctgctcg ggcgccatgt agccaagcgt tccgccgcgc
     1921 acgccgagcg gatggttgcg cgattgcgcg aggccccagt cgacgagcaa gacctcgccg
     1981 taatcaccga gcagaatgtt cgccggtttg atgtcgcaat ggatcacgcc gcgatgatgc
     2041 gcgtagtcga ccgcgttgca gacctgaagg aagtaacgta gcaggcggtt gagcgtgtag
     2101 tcgtgcgggt tgccctcgcc ggtcttgcgc tggtgcaaga tcatctcgag cgacgtctcc
     2161 gtgaccttgc gcatcacgta gaacggacca cgcacgttgt cggtgcccgc gtcgtagatc
//
LOCUS       DASUFX010000160         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_998161,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000160 DASUFX010000000
VERSION     DASUFX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998161"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..764
                     /locus_tag="VFV99_06360"
     CDS             54..764
                     /locus_tag="VFV99_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058962.1"
                     /translation="MRWAALVLCVVVVVACKKKDKEPEPAAMPTTPSTGHSSTKIADQ
                     PEPEPGSAHAGGSAVAHAPAAAGEEAERPTHGGNGQPAYRDEGGHVKGPGGPLFMGHS
                     DECTDKIDHCLREGVWFAVGNVQRGHIYRATPVFQFEDKWWSWRGDPETDWQTLYRTK
                     VAESSSDVRAGSPVIWLIEENSSSKWLVSEFDALTTSRWEAGIVESVGGSTIKVDGWD
                     HPISLDTVRVIVQQKKAG"
     gene            795..1394
                     /locus_tag="VFV99_06365"
     CDS             795..1394
                     /locus_tag="VFV99_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058963.1"
                     /translation="MTEVLERGDIGFFFRPAVQPADAVETRLGVQSFFVVLSTASGVH
                     RRVRIGRKRMPAPTGERFWARVERVGSLQRVLGDQLEAEQYTTKTRGERYQPGARHIA
                     QGCYAFVRHDDHMHLVYRVEQVEQDAPEEVRVPDAMSLLVLFERGPRGPRGRAIWSTE
                     GGPPLLDDEGFELVLVGVHDEPERELGIDVLGHPMDHPI"
     gene            1443..2258
                     /locus_tag="VFV99_06370"
     CDS             1443..2258
                     /locus_tag="VFV99_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5058964.1"
                     /translation="MSDPLILGAVAYDAKVVPIWDGFRAWFGARGVDFDYVLYTNYER
                     QVEAHLRGDVHVAWNSPLAWIETVRLARKHGRDASAIAMRDTDCDLTSVVIVRDDSPV
                     KKLTDLRGKRVGVGAGDSPQATLIPLLAFAEAGVDVEAVRHDVLLGKHGDHIGGERDA
                     VKALLAGTVDAACVLDGNRVGFAREGLGGTRVVHATGQYDHCNMTVLDRAPTSVTKFR
                     ELLLSMSYDDPEVRSLLDMEGLKVWQPGRTTGYALLERACDRFGTLETWLAAR"
     gene            2255..3415
                     /locus_tag="VFV99_06375"
     CDS             2255..3415
                     /locus_tag="VFV99_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058965.1"
                     /translation="MTHAIDLEDLGVDEGAHLLVKRALRDHEQVLVRGESPSLAVDLQ
                     AWCRGEGHKLERGNGVITILRSPHDRWRTAERAGSVDRVADYAPARWGLAARGALVES
                     GVRDIGLPLAYKLDVWADEAARMYQQAAALQWDPATAIPWDAPIDHPEEVEDAVVQVM
                     TYLIENETAALLVPARFLGRLHPHFREVMQLLAIQTADEARHIEVFTRRARLRRTELG
                     LSTAGGQASLATLFDEPDFAIASFLLSVLGEGTFLSLLWFLRDHAPDLCTREVARLAA
                     QDEARHVAFGLAHLGRHVQEDPAIRSRLAAATERRHGALANTAGLNREVFDALVLLAA
                     GGWSPAQLRHGHQAVVALVGEMDQARSQRLIRLGFEPAQAALLSSLHTRNFM"
     gene            complement(3421..3858)
                     /locus_tag="VFV99_06380"
     CDS             complement(3421..3858)
                     /locus_tag="VFV99_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX5058966.1"
                     /translation="MSKAFTKDDEGTPPPPMRKRGAPVPDPNFVTPAGARAARAELEQ
                     LVRDGGDPDRIRELTEHLATAQEVSPDDRTVVGLGATVTLETDGAKRTTYRIVGAIEA
                     DTKRGWLSWQSPIANLLWGARVGDTVELPRGDEAEILAINYDE"
     gene            4082..4471
                     /gene="speD"
                     /locus_tag="VFV99_06385"
     CDS             4082..4471
                     /gene="speD"
                     /locus_tag="VFV99_06385"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX5058967.1"
                     /translation="MTSLGRHLLGEYHGCDRELLNRPEHIRAEMLAAVARSGATVIQD
                     FVHTFTPHGVTGIVVIAESHFAIHTWPEYGFAAVDLFTCGDTVDPWRAFEHLASALRA
                     QTHSVVEMRRGLMPSRDGQRLPHKPGA"
     gene            complement(4468..>5031)
                     /locus_tag="VFV99_06390"
     CDS             complement(4468..>5031)
                     /locus_tag="VFV99_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058968.1"
                     /translation="TREVSLQLLPGFDAAFMVIADDPSALYRVWTRERCEDMLVSFAA
                     GTLRSLGDAIELDWPSDPTIVKVKRGIDFLISLARADVYGMAALRALPDATPTADGYV
                     EIAGPGAIRIGPHRREHSMVTRASASVELDPARTPPPTLGAAALACANGETTIEWLEI
                     EENPTRLTAAIEFLRSLAVAPSEGVFR"
BASE COUNT          830 a         1602 c         1746 g          853 t
ORIGIN      
        1 agcgcgtgaa gtcgacgaac cgcagagacc gtgccctgag gtaggatgca gcaatgcgtt
       61 gggctgccct ggtgctatgc gtcgtcgtcg tcgtcgcctg caagaaaaag gataaggaac
      121 ccgagccggc tgcgatgcca accacgccgt cgaccggcca ttcctcgacg aagatcgctg
      181 atcagcccga gccagaaccc ggcagcgcgc acgctggtgg tagcgcggtg gcgcacgcac
      241 ctgccgccgc gggggaggaa gccgagcggc cgacgcacgg cggcaacggt cagcccgcct
      301 atcgtgacga gggtggtcac gtaaaaggtc ccggcggccc gctgttcatg ggccacagcg
      361 acgagtgcac cgacaagatc gatcactgct tgcgcgaggg tgtgtggttc gcggtcggga
      421 acgtgcaacg cggccacatt taccgcgcga cgccagtgtt ccagttcgag gacaagtggt
      481 ggtcgtggcg cggcgatccc gagacggact ggcagacgct ctaccgcacg aaggtggccg
      541 agtcgtcctc cgacgtacgc gccggctcac cggtgatctg gctgatcgaa gagaactcgt
      601 cgtcgaaatg gctggtcagc gagttcgacg cgctcacgac gtcgcggtgg gaagcgggaa
      661 tcgtcgagtc ggtgggcggc agcacgatca aggtcgacgg ctgggaccac ccgatttcgc
      721 tcgataccgt gcgcgtgatc gtgcagcaga aaaaggccgg ttgacgtcct cgcgtggtgg
      781 tccacgctgg agacatgacc gaggtcctgg agcgaggcga catcggcttc ttcttcaggc
      841 ccgcggtgca acctgcggac gcggtcgaga cgcggcttgg cgtgcagagc ttcttcgtcg
      901 tgctcagcac agcgagcggt gtccaccggc gcgtgcggat cggccgcaag cggatgccag
      961 cgccgacggg cgagcggttc tgggcgcgcg tcgagcgcgt cggctcgctg cagcgcgtac
     1021 tcggtgatca gctcgaagcc gagcagtaca cgacgaagac acgcggcgag cgttaccagc
     1081 cgggtgctcg gcacatcgcg cagggttgct acgcgttcgt acgccacgac gaccacatgc
     1141 atctcgtgta tcgggtcgaa caagtcgagc aggacgcgcc agaagaggtg cgggttcccg
     1201 acgcgatgag tctcctcgtc ctgttcgagc gcgggccacg cgggccgcgc gggcgcgcga
     1261 tctggagcac cgagggcgga ccgccgctgc tcgacgacga agggttcgag ctggtcttgg
     1321 tcggtgtcca cgacgagccc gaacgcgagc tcggcatcga cgtgttgggg caccctatgg
     1381 accacccgat ctagggaaat cgccgtcgac agcgacgggg cccgtgctag cgtgaccgcc
     1441 gcatgagcga tccattgatt ctcggtgcag ttgcgtacga cgcgaaggtc gtaccgatct
     1501 gggacggctt ccgcgcatgg ttcggcgcgc ggggagtcga tttcgactac gtcctctata
     1561 cgaactacga gcgccaggtg gaggcgcatc tgcgcggcga tgtccatgtt gcgtggaact
     1621 cgccgctcgc gtggatcgag accgtgcgac tcgcgcgcaa gcatggccgc gacgcgagcg
     1681 cgatcgcgat gcgcgacacc gattgtgatc tgacgagcgt cgtgatcgtg cgcgacgaca
     1741 gccccgtgaa gaagctcacc gatctgcgcg gcaagcgcgt cggtgttggc gcgggagatt
     1801 cgccgcaagc gacgctgatc ccgctgctcg catttgcaga ggcaggcgtc gacgtcgagg
     1861 cggttcgtca tgacgtcctg ctcggcaagc atggcgatca catcggaggt gaacgcgatg
     1921 cagtgaaggc gttgcttgcc gggacggtcg atgctgcatg cgtcctcgat ggaaaccgag
     1981 tcggattcgc gcgcgaagga ctcggcggca cgcgcgtcgt gcacgcgact ggccagtacg
     2041 atcactgcaa catgaccgtg ctcgatcgcg ccccgacgag cgtcacgaag tttcgcgagc
     2101 tgttactctc gatgtcgtac gacgatccag aggtcaggag cctgttggat atggaaggac
     2161 tgaaggtgtg gcaaccggga cgcacgaccg gctacgcgtt gctcgagcgc gcgtgtgacc
     2221 ggttcggcac gctggagacg tggttagcag cgcgatgaca cacgcgatcg atctcgagga
     2281 cctcggcgtc gacgaaggag cacacctcct cgtcaagcgc gcgctgcgtg atcacgagca
     2341 ggtgctcgtg cgcggcgagt cgccgagcct cgcggtcgat ctgcaagcat ggtgtcgtgg
     2401 ggaggggcac aagctcgagc gcggcaacgg cgtcatcacg atcctgcgca gtcctcatga
     2461 tcgctggcgc acggccgagc gggcagggag cgtcgatcga gtcgcggact atgcgcctgc
     2521 acgctggggc ctggcagcgc gcggcgcgct cgtcgagtcc ggtgtgcgcg acatcggcct
     2581 gccgcttgcg tacaagctcg acgtgtgggc cgacgaagcc gcacggatgt accagcaggc
     2641 cgccgcgctg caatgggatc cggcgaccgc gattccgtgg gacgcaccga tcgatcaccc
     2701 cgaggaggtc gaggacgcgg tcgttcaggt gatgacgtat ctgatcgaga acgaaaccgc
     2761 ggcgctgctc gtgcccgcgc ggttcctcgg ccgcctgcat ccgcactttc gcgaggtgat
     2821 gcagctgctc gcgatccaga ccgccgacga ggcgcggcat attgaagtat ttacgcggcg
     2881 cgcccggttg cgccgtaccg agctcgggct atcgaccgca ggtggccaag cgtcgctcgc
     2941 gacgctattc gacgagccgg acttcgcaat cgcgagcttc ctgctctcgg tgctcggcga
     3001 aggcacgttc ctgtcactgc tctggttcct ccgcgaccac gcgcccgatc tgtgcacgcg
     3061 cgaggttgcc cgacttgccg cgcaggacga agcgcgccac gtcgcgttcg ggctcgcgca
     3121 cctaggccgt cacgtgcagg aggatcccgc gattcgtagt cggctcgcgg cggccaccga
     3181 gcgccggcat ggtgcgctcg cgaacaccgc ggggctcaac cgcgaggtgt tcgacgcact
     3241 cgtgttgctc gctgcaggcg gctggtcgcc ggcgcagttg cggcacggtc accaggccgt
     3301 ggtcgcgctc gttggcgaga tggaccaggc ccggagccag cggttgatcc gactcggctt
     3361 cgagcccgcg caggcggcgc tactgtcgtc gctgcacacg cgaaacttca tgtaagcgag
     3421 ctactcgtcg tagttgatcg cgaggatctc ggcttcgtca ccgcgcggaa gctcgacggt
     3481 gtcgccgacg cgcgcgcccc acagcagatt cgcgatcggc gattgccagc tcagccagcc
     3541 gcgcttggta tcggcttcga tcgcaccgac gatgcgatag gtcgtgcgct tagcgccatc
     3601 ggtttcgagc gtcaccgtcg caccgaggcc gaccactgtg cggtcgtcag gcgagacttc
     3661 ctgagcagtc gcgagatgct ccgtgagctc gcggatgcga tccggatcgc cgccatctcg
     3721 gactaactgc tcgagctccg cgcgagcagc gcgagctcct gcgggcgtta cgaagttggg
     3781 atcaggaacc ggcgccccgc gcttacgcat tggcggcggc ggtgttccct cgtcgtcctt
     3841 cgtaaaagct ttgctcacgt gctccagtct accgctgggc atgtcgattg ctccctacgc
     3901 ggttgggagg ttatcgcgat gtctacaact gcttggatcg ttgttctcgt cttgctcgct
     3961 ctactcctcg gtggcggtgg ctacttctac cgtcggtgag cgaataggca ctctgggcgg
     4021 gtgctctgag agcattcgct agacagcacc cggcgtcatc gccgacagta ccgcgatggc
     4081 gatgacgtcc ctgggcagac atctgctcgg cgaataccac ggatgcgatc gcgagcttct
     4141 caaccgcccg gagcacattc gggccgagat gttggcggcg gtcgcgcgct ccggcgcgac
     4201 ggtgatccag gacttcgtgc acacgttcac gccgcacggg gtcaccggca tcgtcgtgat
     4261 cgccgaatcc cacttcgcga tccacacctg gccagagtac ggattcgccg cggtcgatct
     4321 gttcacctgc ggcgacaccg tcgatccatg gcgcgcgttc gagcacctcg cgtcggcact
     4381 gcgcgcgcag acgcactcgg tcgtcgagat gcgccgcggt ctcatgccgt cgcgcgatgg
     4441 ccaacgcctg ccacacaagc cgggcgccta gcggaagacg ccttcactcg gcgcgaccgc
     4501 gaggctgcgg agaaactcga tcgcggcagt gagccgggtc ggattctctt cgatctcgag
     4561 ccactcgatc gtggtctcgc catttgcgca ggcgagtgcg gccgctccga gcgtgggcgg
     4621 cggtgtgcgc gccgggtcga gctcgacgct cgccgatgca cgcgtgacca tgctgtgttc
     4681 gcggcgatgc gggccgatgc ggatcgcgcc gggccccgcg atctcgacgt aaccgtcggc
     4741 ggtcggagtg gcatcgggga gcgcgcggag cgccgccatc ccgtagacgt cggcgcgcgc
     4801 gaggctgatc aggaaatcga tgccgcgttt caccttcacg atcgtcgggt cgctcggcca
     4861 gtccagctcg attgcgtcgc ccagcgagcg gagcgtgccg gccgcgaatg aaacgagcat
     4921 gtcttcacac cgttcacgcg tccatacgcg atagagcgcg gacgggtcgt cagcgatgac
     4981 catgaacgct gcgtcgaagc cgggcagcag ctggaggctg acctcgcgcg t
//
LOCUS       DASUFX010000161         8816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_302783,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000161 DASUFX010000000
VERSION     DASUFX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302783"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFV99_06395"
     CDS             <1..1336
                     /locus_tag="VFV99_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5058969.1"
                     /translation="FRKAIAASIDEIAGVLHRENGKPELEAYVEVMMALNHVQHAAAH
                     AEKAMEPKKVGAGMMANFRATVSYHPLGVVGVIGPWNYPLFTPMGSIAYALAAGNAVV
                     WKPSELTPLVALEIEKIARRTFALPDLLQVVTGAGTTGAALAKSAVDKIAFTGSAATG
                     KRVMMAAAERLTPVLLELGGKDAMIVDSDADIEKAAEAAVYGGLTNCGQACVSVERVY
                     VADAVYDRFVDEVVKQVRELKVGGDDGDIGAMTSPAQVSIVKDHLEDAIAKGAKVLTG
                     GPDAISGSYIQPTVLTNVDHRMKVMTEETFGPVIPIAKVASVDEAVKLANDSRYGLGS
                     SVFAGKAARKIASQLRAGMTAVNSVMAFAGIPNLPFGGIGDSGFGRIHGDEGIREFTR
                     IKSTAEQVMSIPLNMMSFRQPKDAVKRMRGMVKQLYGDGVVSKAGDWFRKLR"
     gene            1333..2133
                     /locus_tag="VFV99_06400"
     CDS             1333..2133
                     /locus_tag="VFV99_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058970.1"
                     /translation="MRWLLITALVGFGCRPQHVKQHTCDKPIGGLATYLKPGAIVWFG
                     EMHGTEESPRFVGDAACEAAKVAHVQLGLEIPHAEQPAFDRYLKSKGSDADRAALVAG
                     PFWQQADGRSSLAMVALVERVRVLRAAGAPIDLVLYDDPTAGRDEAMAKMVMRLRDPK
                     AIFVALSGNVHSRRTKGARWDPNLVPTVAHLVAAKLPVTTFNVSAAGGTLWACMSGPD
                     EEPECGEHPNSNDGAGTPWTLGPPRDDAHDGVYFVGATQASYPAKQSR"
     gene            complement(2152..2961)
                     /locus_tag="VFV99_06405"
     CDS             complement(2152..2961)
                     /locus_tag="VFV99_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5058971.1"
                     /translation="MRSLVVALALLAFATTVDAKPRRAKKSIATKNEKSTKKRAKQAD
                     RSSRKRKRVGMTTHKHPHFEIRGPIVGQSVGAPWSGRLRDAAKLAESDTWYIRRPYRS
                     YGTRTTVDFVQRVLSDIADRFPDIHKIAIGDISAETGGQISDHSSHQSGRDIDIGLIF
                     SEKPDGYPNSFVVGTEDNLDLEATFVMVEEFAKTTREQGGVLMIFLDFNVQKLLYDWA
                     LENGESEDYLGKLFQYPHGRGSSEGIVRHEPNHRDHIHVRFRCPAGDSACR"
     gene            complement(2961..4040)
                     /locus_tag="VFV99_06410"
     CDS             complement(2961..4040)
                     /locus_tag="VFV99_06410"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5058972.1"
                     /translation="MKSAVAVVAVLAIALLSGCLYTSYVTQAAQGQFQLLGKAKPIDQ
                     VVNDPDTPLRVAILLAEIPSIKMYGRRYGLKIRKNYETYSSLGRPAAVWFTAAADPVA
                     FKPRKWCFPIVGCFAGLGWFDEDEAVAFKQKMEAAGYDAIVRPASAYSTGGWFPDPVL
                     STMLGGGDDALPELFNVIIHESVHATVLVPDEPFFNESLASYVADTLTDAWIAERFGP
                     GSPEHVAWTLGQALHLPRVARLLAAYKALKTVYESKATREQKLASKAKIIDELVADLK
                     LRKRPNNASLTEVRVYNGGTAPLILAHRACGDLRALVESAKTLRRSDFQKSLQEDLTG
                     IGQLIGQRCATKKLDQGIKSAHDSR"
     gene            complement(4037..4444)
                     /locus_tag="VFV99_06415"
     CDS             complement(4037..4444)
                     /locus_tag="VFV99_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5058973.1"
                     /translation="MAETIFGKILRGEIPCHRVYEDDKVLAFLDVNPIARGHVLVIPR
                     EPAETIDKLSDDSAAALGRVLPRLARAVMAVTGTKAYNVLQNNGPLAHQAVMHVHFHI
                     IPKYDDGTGLGIGWNATKLDGGAELAKAIASNL"
     gene            complement(4466..5542)
                     /locus_tag="VFV99_06420"
     CDS             complement(4466..5542)
                     /locus_tag="VFV99_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058974.1"
                     /translation="MKRLLYVGFLSLPLVGCYGGERRESGKCPAGELCSDLTPGGLDF
                     VGNGLIGELLLSGPRPTAIGGTQAVALEYDRGDGVDVALDLPFSADDDGAAGVKVDAT
                     SGSQVIVRGMGSRSNYLRILDSDGLLMDRKELTGAALDTMELVPGWFESVPADKLIAF
                     APGKRELGVALYGDVQGTGGPRSERLIDTSMELTLAGSTRKAWDTLEVEAVVGTTSLA
                     VTAGDKPTANLDIVVADHADTVSLIQPAPTTVMPNSSASVCFQANTGGRYLAGLNWTY
                     VVDGGEPVVKGDGIFTRNCISVSTQKTSGIIIVQASAGGQSAQTQLTIGAGARTTPRE
                     SVLAGTPVRAASHATATAGDRAAM"
     gene            complement(5660..6358)
                     /locus_tag="VFV99_06425"
     CDS             complement(5660..6358)
                     /locus_tag="VFV99_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5058975.1"
                     /translation="MTSRILIVEDDDAIATGLALNLKLAGRTTTIARDGDDAIRQATD
                     EDFALILLDINLPKKNGLEVLSLLRATDNLVPVIVLSARDGEYDKVAALRLGADDYVT
                     KPFALAELLARIDAVLRRTNQLTTVPVAAPPSEQLTFDDVVIVPAQRTAARAGVELKL
                     THLEFELLLFFVMHPNQVFSRTQLFNLVWGQSAGSVRTVDNFVGQLRKRFEADPEQPR
                     HFITVRGSGYRFDP"
     gene            complement(6355..7308)
                     /locus_tag="VFV99_06430"
     CDS             complement(6355..7308)
                     /locus_tag="VFV99_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5058976.1"
                     /translation="MRFPPATLQLRRAQLILMLVALLPTVAMSVVGILLLVLGSGSTH
                     NIVAGVLVLTFCTSGITGYILGSVYLGKGASLVRVQNDFVSAVSHELRTPVTSIRLLL
                     ESLRDGRLADDDRKQVLTLLARETTRLEQLVGRVLELSRMESGAHVYARDRVDIKELV
                     DEAIAAFDASTLDNPTPIEIELEPGLAIIGDRPTLVRAILNLLTNAWKYTGEKKRIVI
                     EATGAARWVELRVRDNGPGIDRDEQRSIYEQFKRGRAAHESGKAGVGLGLAFVRTIVR
                     GQRGKLDFDSKPGETTFRMRLRRAREPVPQGAPALGEKVTS"
     gene            complement(7316..8257)
                     /locus_tag="VFV99_06435"
     CDS             complement(7316..8257)
                     /locus_tag="VFV99_06435"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX5058977.1"
                     /translation="MTVTPVETARAKGWRHVYFNTLSLPFWGVHILAIIGIAITGFSW
                     LGLLWCAALYWPRMFFVTGAYHRFFAHRSYKTSRWFQFVLALGATFTGQKGPLWWAAH
                     HRVHHKYSDLPGDLHSAKRDGFWWSHMGWILARDLEGTDLSRIKDFSKYPELRWLDRY
                     WMLPPVAAGVIAFLIGGFFGLIWAFAVPQVLCWHGTFTINSLSHLWGGRRYATEDDSR
                     NNVVLALITMGEGWHNNHHHYQVAARQGFYWWEIDCTYYVLRALSAVGLIWDLHGVPD
                     HIIQASDLSPQASGSESAEALDSGRGLKPEARGLNVA"
     gene            8329..>8816
                     /locus_tag="VFV99_06440"
     CDS             8329..>8816
                     /locus_tag="VFV99_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5058978.1"
                     /translation="MQYRNLGGAGVKVSSLCLGAMTFGEADEKSFMHKVGMDEATSHA
                     VLDRALAAGINFIDTADVYGQDGLSERVLGNWLSTRGTRDKIVLATKFRFTMGDGPNR
                     SGASRFRIVKCCEDSLRRMKTDRIDLYQIHMQDITVPEEETLRALDDLVSAGKILYTG
                     CS"
BASE COUNT         1539 a         2735 c         3013 g         1529 t
ORIGIN      
        1 atttcgcaag gcgatcgccg cgtcgatcga cgagatcgcc ggagtcctgc accgcgagaa
       61 cggcaagccc gagctcgagg cgtacgtcga agtgatgatg gcgctcaatc acgtgcagca
      121 cgcggctgca cacgccgaga aggcgatgga gccgaaaaag gttggcgccg gcatgatggc
      181 gaacttccgc gcgacggtca gctatcaccc gctcggtgtg gtcggcgtga tcggtccgtg
      241 gaactacccg ctgttcacgc cgatgggctc gatcgcgtac gcgcttgccg ccggtaacgc
      301 ggtcgtgtgg aagcctagcg agctgacgcc gctcgtcgcg ctcgagatcg agaagatcgc
      361 gcgtcgcacg ttcgcgctac ccgatctgct ccaggtcgtg acgggcgcgg gtaccacggg
      421 tgccgcgctt gcgaagtccg cggtcgacaa gatcgcattc accggctccg cagcgacggg
      481 caagcgcgtg atgatggcgg cggccgagcg gctgacgccc gtgctgctcg agctcggcgg
      541 caaggacgcg atgatcgtcg actccgacgc cgacatcgag aaggccgcgg aagctgcggt
      601 ctacggcggg ctcacgaact gcggccaggc ctgcgtctcg gtcgagcgcg tctacgttgc
      661 cgatgccgtc tacgatcggt tcgtcgacga ggtcgtcaag caagtgcgcg agctcaaggt
      721 cggcggcgac gacggcgaca tcggtgcgat gacgagcccg gcgcaggtct cgatcgtcaa
      781 ggatcatctc gaggacgcga tcgcgaaggg cgcgaaggtg ctcaccggcg gtccggacgc
      841 gatcagcggc agctacatcc agccgaccgt gctcacgaat gtcgatcacc gcatgaaggt
      901 gatgaccgag gagacgttcg gtccggtgat tccgatcgcg aaggtcgcct cggtcgacga
      961 ggccgtgaag ctcgcgaacg attcgcgcta cggcctcggc tcgtcggtgt tcgccggcaa
     1021 agcggcgcgc aagatcgcga gccagctccg cgcgggcatg accgcggtca actcggtgat
     1081 ggcgtttgcc ggcattccga acctgccgtt cggcggcatt ggcgattcgg ggttcggccg
     1141 cattcacggc gatgaaggca tccgcgagtt cacgcgcatc aagtcgacgg cagagcaggt
     1201 gatgtcgatc ccgctcaaca tgatgtcgtt ccgacagccc aaggatgccg tcaagcgcat
     1261 gcgcggcatg gtcaagcagc tgtacggcga cggcgtcgtg tcgaaggccg gtgactggtt
     1321 ccgcaagctg cgatgagatg gctgctgatc accgcgctcg tcggcttcgg atgccggccg
     1381 cagcacgtga agcagcacac gtgtgacaag ccgatcggtg ggctcgcgac gtatctgaag
     1441 cccggcgcga tcgtgtggtt cggcgagatg cacggcaccg aggagtcgcc gcgctttgtc
     1501 ggcgatgccg cgtgcgaggc ggcaaaggtc gcgcacgtgc agctcgggct cgagattccg
     1561 cacgccgagc agccggcatt cgatcgctat ctgaagagca aggggagcga cgcggatcgt
     1621 gcggcgctgg tcgccggacc gttctggcag caagcggatg gccggagcag cttggcgatg
     1681 gtcgcgctcg tcgagcgcgt gcgtgtgctt cgagccgccg gcgcgccgat cgatctcgtg
     1741 ctctacgacg atccgacggc gggtcgcgac gaggcgatgg cgaagatggt gatgaggctg
     1801 cgtgatccga aggcgatctt cgtcgcgctg tcgggcaacg tgcattcgcg gcgcacgaag
     1861 ggcgcgcggt gggatccgaa tctcgtcccg accgtcgcgc atctcgtggc tgcgaaactg
     1921 ccggtgacga cgttcaacgt atcggcggcg ggcggcacgc tgtgggcctg catgagcggt
     1981 cccgacgaag aaccggagtg tggcgagcat ccgaatagca acgatggcgc aggcacaccg
     2041 tggacgctcg gcccgccgcg tgatgacgcg cacgacggtg tgtatttcgt cggtgcgacg
     2101 caggcgtcgt atccggcgaa gcagtcgcga taagatcgag cgcgacgggc gctagcgaca
     2161 ggcgctgtcg ccggccgggc agcggaagcg cacgtgaatg tgatcgcgat ggttcggctc
     2221 gtggcgcacg atgccttcgc tcgatccgcg gccgtgcggg tactggaata gcttgccgag
     2281 gtagtcctcg ctctcgccgt tctcgagcgc ccagtcgtac aggagcttct gcacgttgaa
     2341 gtcgaggaag atcatcagca cgccgccttg ttcgcgtgtc gtcttcgcga attcctcgac
     2401 catcacgaac gtcgcctcga gatcgaggtt gtcctcggta ccgacgacga aactgtttgg
     2461 atagccatcg ggcttctcgc tgaagatgag accgatgtcg atgtcgcgcc ccgattggtg
     2521 cgagctgtgg tcgctgatct ggccgccggt ttcggccgag atgtcgccaa tcgcgatctt
     2581 gtggatgtcg gggaagcgat cggcgatgtc gctgagcacg cgctgcacga agtcgacggt
     2641 cgtgcgcgtg ccgtacgagc gatacgggcg gcggatgtac caggtgtcgc tctcggcgag
     2701 ctttgcggca tcgcgcaggc gacccgacca cggtgcgccg acgctctgac cgacgatcgg
     2761 gccgcggatc tcgaagtgcg gatgcttgtg cgtcgtcatc ccgacgcgtt tgcgtttgcg
     2821 gctggaccga tccgcctgct tcgcgcgttt cttcgtcgac ttctcgttct tcgtcgcgat
     2881 cgatttcttg gcgcgtctgg gctttgcgtc gacggtggtg gcgaatgcga gcaaagcaag
     2941 agctacgacg agcgagcgca tcacctggaa tcgtgagcac tcttgattcc ctgatccagt
     3001 ttttttgtag cgcagcgttg accgatcagc tggccgatac ccgtgaggtc ttcctgcagc
     3061 gacttttgga aatcgctgcg ccggagtgtc ttcgcactct cgacgagcgc acgtagatca
     3121 ccgcaggcgc ggtgcgcgag gatgagtggc gccgtaccgc cgttgtagac gcgtacttca
     3181 gtaagcgacg cgttgttcgg ccgcttgcgc agcttcagat ccgcgacgag ctcgtcgatg
     3241 atcttcgctt ttgacgcgag cttctgctcc cgcgttgcct tggactcgta gacagtcttg
     3301 agcgccttgt atgcagcgag caacctggca actcgtggca ggtgcaatgc ctgaccgagt
     3361 gtccaggcaa catgctcggg tgacccgggg ccgaaccgct ccgcgatcca ggcgtcggtg
     3421 agcgtatccg ccacatacga ggccaagctc tcgttgaaga acggctcgtc tgggaccagg
     3481 acggtcgcgt ggaccgactc gtggatgatg acgttgaaca gctcggggag ggcgtcgtca
     3541 ccgccgccga gcatcgtcga cagcaccggg tcggggaacc agccgccggt cgagtaggcg
     3601 gaggccgggc ggacgatcgc gtcgtaaccg gccgcctcca tcttctgctt gaacgcgacc
     3661 gcctcgtctt cgtcgaacca gccgaggccg gcgaagcagc cgacgatcgg gaagcaccac
     3721 ttgcgcggct tgaacgcgac cgggtcggcg gcagccgtga accacacagc ggcgggacgg
     3781 ccgagcgagg agtaggtctc gtagttcttg cggatcttga ggccgtagcg gcggccgtac
     3841 atcttgatcg atggaatctc ggcgagcagt atcgcgacgc ggagcggcgt gtccggatcg
     3901 ttcaccacct gatcgatcgg tttggccttg ccgagcagct ggaactgccc ctgcgcggcc
     3961 tgggtgacgt agctcgtgta caggcagccc gagagcaggg cgatcgcgag gacggcgacg
     4021 accgcgacgg cggacttcac aggttgctcg cgattgcctt cgccagctcg gcgccaccgt
     4081 cgagtttcgt cgcgttccag ccgatgccga gcccggtgcc gtcgtcgtac ttcgggatga
     4141 tatggaagtg gacgtgcatc acggcctggt gcgcgagcgg accgttgttc tgcagcacgt
     4201 tgtaggcctt cgtgccggtc accgccatca ccgcgcgggc gaggcgcggg agcacgcggc
     4261 cgagcgcagc ggccgagtca tccgagagct tgtcgatcgt ctcggcaggc tcgcgcggaa
     4321 tcacgagtac gtggccacgc gcgatcggat tgacgtccag aaacgcgagg accttgtcat
     4381 cctcgtagac gcgatgacat gggatttcgc cgcgcaggat ctttccgaaa atcgtctcgg
     4441 ccatgcgtcg agactagcgc atcaatcaca tcgctgcgcg gtcacccgcg gtcgcggtcg
     4501 cgtgcgaggc ggcgcgcacc ggcgtacccg ccagcactga ctcccgcggc gtcgtgcggg
     4561 cgcctgcgcc aatcgtgagc tgggtctgcg ccgactgacc acccgcgctc gcctgcacga
     4621 tgatgatgcc cgacgtcttt tgcgtggaca cgctgatgca gttacgggtg aagatgccgt
     4681 cgcctttgac gaccggttcg ccgccgtcga ccacgtacgt ccagttcaac ccggcgagat
     4741 agcgaccacc ggtgttcgcc tggaagcaga ccgatgccga gctgttcggc atgaccgtgg
     4801 tcggcgctgg ctggatcagc gagacggtgt ccgcatggtc ggcgaccacg atgtcgaggt
     4861 ttgcggtcgg cttgtcgcct gcggtgaccg caagtgatgt cgtgccgacg acggcttcga
     4921 cctcgagcgt gtcccacgcc ttgcgcgtcg agccggcgag cgtgagctcc atcgacgtat
     4981 cgatcaagcg ctcgctccgg gggccacccg ttccctgcac gtcgccatag agcgcgacgc
     5041 caagttcacg cttgcccggc gcgaacgcga tcaacttgtc ggccggcacg ctctcgaacc
     5101 agcccggcac cagctccatc gtgtcgagcg ccgcaccggt cagctccttg cggtccatca
     5161 gcaagccatc gctatcgagg atacggagat agttgctgcg actacccatg ccgcgcacga
     5221 tgacctgcga gccactcgtc gcatcgacct tcacgccggc ggcgccgtca tcgtcggccg
     5281 agaacggcag gtcgagcgcg acatcgacac cgtcgccgcg gtcgtactcg agcgcgacgg
     5341 cctgggtgcc gccgatcgcg gtcggccgcg ggccgctgag caagagctcg ccgatgagcc
     5401 cattgccgac gaagtcgaga ccacccggcg tcaggtcgga gcacagctcg ccggctggac
     5461 acttgccaga ttcgcggcgc tcgccgccgt agcaaccgac cagtggcaaa gacaggaacc
     5521 ctacataaag gaggcgcttc atggtcgtcg gaaagagcac cggttgtgcc atggccggac
     5581 cagccagctt tcgccgagtt ggccgccgcg cgagcacggc ttgccacacg ttccggatcg
     5641 gaatgtcagg gcctacgact cacggatcga aacgatagcc cgagccgcgc acggtgatga
     5701 aatgccgcgg ctgctcggga tcggcttcga agcgcttgcg gagctgaccg acgaagttgt
     5761 cgaccgtgcg cactgaccct gctgactgtc cccacacgag gttgaacagc tgggtgcgcg
     5821 agaacacctg gttcgggtgc atcacgaaga acagcagcag ctcgaactcg aggtgcgtga
     5881 gcttgagctc gacaccggcg cgtgcggcgg tgcgctgcgc gggaacgatg acgacatcgt
     5941 cgaaggtgag ttgctcgctc ggcggcgccg cgaccggcac ggtggtgagc tggttcgtgc
     6001 ggcgcaacac cgcatcgatg cgcgcgagca attcggcgag cgcgaatggc ttcgtcacgt
     6061 agtcgtcggc gccgagtcgc agcgcggcga ccttgtcgta ctcgccatca cgcgcagaca
     6121 gcacgatgac gggcacgagg ttatcggtcg cgcgcaagag cgacagcact tcgagcccgt
     6181 tcttcttcgg caggttgatg tcgagcaaga tgagcgcgaa atcctcgtcc gtcgcctggc
     6241 ggatcgcgtc gtcgccgtcg cgcgcgatcg tggtcgtgcg gccggcgagc ttgaggttga
     6301 gcgcgaggcc cgtcgcgatc gcgtcgtcgt cttcgacgat caagatgcgt gacgtcacga
     6361 cgtcaccttc tcgccgagcg cgggggcgcc ttgcggaacc ggctcacgcg cgcgccgcag
     6421 ccgcatccgg aacgtggtct cgcctggctt cgaatcgaag tcgagcttgc cgcgctggcc
     6481 gcgcacgatc gtgcgtacga acgcgaggcc gagaccgacg cccgctttgc cgctctcgtg
     6541 ggccgcgcgg ccgcgcttga actgctcgta gatcgagcgt tgctcgtcgc gatcgatgcc
     6601 aggaccgtta tcgcggacgc ggagctcgac ccagcgcgct gcgcccgtcg cttcgatcac
     6661 gatgcgcttt ttctcgccgg tgtacttcca cgcgttcgtc agcagattga ggatcgcgcg
     6721 cacgagcgtc ggacggtcac cgatgatggc gaggcccggt tccagctcga tctcgatcgg
     6781 cgtcgggttg tcgagtgtgc tcgcgtcgaa cgctgcaatc gcttcgtcga cgagctcctt
     6841 gatgtcgacg cgatcgcgcg cgtagacgtg ggcaccggat tccatgcgcg acagctcgag
     6901 cacgcggccc acgagctgtt cgagtcgcgt ggtctcgcgc gcgagcagcg tcagtacctg
     6961 tttgcggtcg tcatcggcga gccggccatc gcgcagcgat tcgagcagca ggcggatcga
     7021 ggtgaccggc gtgcggagct cgtgggaaac cgccgacacg aagtcgtttt gcacgcgaac
     7081 cagtgacgcg cccttgccga ggtagaccga gccgaggatg tagccggtga tccccgacgt
     7141 gcagaacgtg agcacgagga caccggcgac aatgttgtgc gtggatccgc tgccgagcac
     7201 gagcaacaag atgccgacga cggacatcgc gaccgtcggc agcagtgcga cgagcatcag
     7261 gatcagctga gctcgacgaa gttgcagggt agcgggtggg aaccgcacgg gcggactatg
     7321 ccacgttcag gccgcgggcc tcgggcttca gacctcggcc agaatccaga gcttcggcgg
     7381 attctgatcc tgaggcctga ggactgaggt ctgaggcctg gatgatgtgg tccgggacgc
     7441 cgtgcaggtc ccagatcagc ccgacggcgg acagcgcacg taacacgtag tacgtgcagt
     7501 cgatctccca ccagtagaag ccttgccgcg cagccacttg gtagtggtgg tggttgttgt
     7561 gccagccttc gcccatcgtg atcagggcga gcacgacgtt gttgcgcgaa tcgtcctcgg
     7621 tcgcgtagcg gcggccgccc cacaggtgcg agagcgagtt gatcgtgaac gtgccgtgcc
     7681 agcacagcac ctgcggaact gcgaacgccc agatgaggcc gaagaagccg ccgatcaaga
     7741 acgcgatcac gccggccgcg acaggcggca gcatccagta gcgatcgagc cagcgcagct
     7801 cgggatactt cgagaagtct ttgatgcgcg agaggtcggt gccttcgagg tcacgcgcga
     7861 ggatccaacc catgtgtgac caccagaagc cgtcgcgctt tgccgagtgc agatcgcctg
     7921 gcaggtcgga gtacttgtgg tgcacgcgat gatgcgcggc ccaccacaac ggacctttct
     7981 ggccggtgaa cgtggcgccg agcgcgagca cgaactgaaa ccaccgcgac gtcttgtagc
     8041 tgcgatgcgc gaagaagcgg tgatacgcgc cggtcacgaa gaacatccgc ggccagtaca
     8101 gcgcggcgca ccagagcagg ccgagccacg agaagccggt gatcgcgatg ccgatgatcg
     8161 cgaggatgtg aactccccag aagggcaggc tcagcgtgtt gaagtagacg tggcgccaac
     8221 ctttggcacg agccgtctcg acgggagtca ctgtcatcgc ttaccgcaag gatagaaaac
     8281 gtcgcccaag gcgacggtca cgattccgtc aggagtagat tgcactccat gcaataccgc
     8341 aatcttggcg gcgccggcgt gaaggtgtcg agtttgtgtc tcggcgccat gacgtttggc
     8401 gaggccgacg aaaaatcctt catgcacaag gtcggcatgg acgaggcgac gtcgcatgcc
     8461 gtgctcgatc gcgcactcgc tgcggggatc aacttcatcg atacggccga cgtctatggt
     8521 caggacggcc tgtcggagcg cgtactcggc aattggctga gcactcgcgg cacgcgcgac
     8581 aagatcgtac tcgcgaccaa gttccgcttc acgatgggcg atggaccgaa tcgcagcggt
     8641 gcgtcgcgct ttcgcatcgt caagtgctgc gaggactcgc tgcgtcgcat gaagacggat
     8701 cgcatcgacc tctatcagat ccacatgcag gacatcacgg tgcccgagga agagacgctg
     8761 cgcgcgctcg atgatctcgt gagcgccggc aagatcctct acacgggttg ctcgaa
//
LOCUS       DASUFX010000162        10101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1971254, whole genome shotgun sequence.
ACCESSION   DASUFX010000162 DASUFX010000000
VERSION     DASUFX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971254"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFV99_06445"
     CDS             <1..640
                     /locus_tag="VFV99_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5058979.1"
                     /translation="GVALVGWLTPTVAELVAPGSHATKWIDAHITEEIVGLFAGCSLF
                     VLPGGKDRPALTWREATQIEWGVILLFGGGILLGTLAKSTGLAAEWGHALVDATGASS
                     TWSITVLVTGVSIVLSEATSNTATASMMAPLAGALAAAAGAAPIPAVLGATMGASFGF
                     IMPISTAPNALAYASGRVSIGQMLRVGIVFDGIGFLLIVGSLRVMCPLLGWA"
     gene            686..1501
                     /locus_tag="VFV99_06450"
     CDS             686..1501
                     /locus_tag="VFV99_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5058980.1"
                     /translation="MDVLVIGGGGALGRLVCSQLTARGHRALAIGRRDGNLRDPALIA
                     RAGAPLIINCAGASVQMGLGHGWRGYRAVDVPIGLAAVEAARRTKARLVYVGVHHPPA
                     LRATAYVDAHERVAEAMADIDGAVVRPTGFFSAISGAFLPMAKRGRMFDVGDGSARTN
                     PICERDLAEIVVDVALGGDGPRDVSVGGPEVMTRREMLERVASAAGYRANVTGMPLAL
                     CKLNAALLRAVHPRMGQFVQFVVGLAQHDVIAPACGTTKLGDYVASLPIARAA"
     gene            complement(1498..1791)
                     /locus_tag="VFV99_06455"
     CDS             complement(1498..1791)
                     /locus_tag="VFV99_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058981.1"
                     /translation="MDDESGYDVLEELDAETPVPQVEDEYAIPQLDDEFHDTERMEVA
                     AVREDTYLGWPPAPPLPAEVELAQGTVDSHPPVRKAGMFAAVRRPTTNSETDD"
     gene            complement(1861..2592)
                     /locus_tag="VFV99_06460"
     CDS             complement(1861..2592)
                     /locus_tag="VFV99_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX5058982.1"
                     /translation="MRFGVAIAVALVAMTAYVVMQATQNRDPAVKKKPTPPECKLGPT
                     TAGIDVSYYQGDITWSRVKRAGVQFAFIRVSDGATIVDTKFEANWTGAKRAHVLRGAY
                     QFFRPNESPIEQANLLVRTLRARGAGELPPVIDIEVTDGVPLATVVANARVWIEQVRS
                     QLGVEPIVYTNYGMFRWGGAEPLARQLLWLAHYTEACPSIPPPWQRWTFWQYTENGRV
                     DGIDGAVDLDVFDGTSDELRRRFGL"
     gene            2673..3917
                     /locus_tag="VFV99_06465"
     CDS             2673..3917
                     /locus_tag="VFV99_06465"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5058983.1"
                     /translation="MALRLGGLADLDPSAVPLPAGTEVTTRVDRAVDGGEMRPQGATG
                     RVAKLDGDRVEVVFLDGKRATYLRGEVMPRKLGVVRYAQRRAAAWELLHPCVAIDTVV
                     GSRAWGVADAGSDEDHRGVFVLPMPWTTGLVDPPLDLVSADGSSTYWELGKAIRQALR
                     ADPNTLEMLFASPAIVDPIGEPLVAARSAFLSHEIYGSFGRYALSQLDRLEHNQRLAE
                     HRVTVIGWLRDDPDLALDTAAERLADAARIEAPSRADAIGRARDYIKQLYRSMYDQGL
                     IAANDWASLRAVARTDLELPRDLRPKNAYNLIRLLDLAIRWLAGESPDVRVSDELRPT
                     LVAIKRGELPMPDVMALARALTPKLEAARQTSPLPRYPDVAVADRVLRAARDEAARRW
                     VQREPGPWGAAAPPPPEAHYDE"
     gene            3907..4779
                     /locus_tag="VFV99_06470"
     CDS             3907..4779
                     /locus_tag="VFV99_06470"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5058984.1"
                     /translation="MTSDIVILSPHLDDAVLSLGGMIGREIAAGRSVEVVSCFTAGPP
                     LDTIAPAHRVFGDYSMRRAEDERALAVLGARHRWLDLHERIWRQPPLPPSGVFPTHVF
                     RTPERMEDFAELKTIRAAIAEVLDGGATTVYAPLAIGHHVDHVEVALAALREVLGRGA
                     FERIHFYEDPYALGRACRKQHFVAKRRMWRPFGAPAWASPRVGGLLRMVAFVAKGPKL
                     EDYLPEADRLEWTCTPHEIAFADEERKLTAISEYVSQVRAFGGMERVAAFMRRAHKVL
                     GGEPLWSCRPAMPR"
     gene            4821..5438
                     /locus_tag="VFV99_06475"
     CDS             4821..5438
                     /locus_tag="VFV99_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058985.1"
                     /translation="MKVWLLAALLVVSTAHADTFELRDVPSTTNRPPFVTRTRDCNPN
                     GVSQMRTLTATIGGYEVELHRDCMAAPQMSSLTVRIHEDWFVAEGFTLTDFGGNMSDS
                     RIEIHRVSESLVKGTFADGSPAIVYRAVTRTDFVSSCDGDDCVDPVTESVKHDNFLVC
                     SVAMETVRCGRIEYECPATGCGAPAFVLGQLVAIEQRTYVPARRN"
     gene            5458..6246
                     /locus_tag="VFV99_06480"
     CDS             5458..6246
                     /locus_tag="VFV99_06480"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5058986.1"
                     /translation="MSFDPWSVLTAKQATIARAFLADRERERKHLVIYLSGAHAYGFP
                     SPDSDLDLKCVHIAPTHDLVGLDVVADPNDRIDIVDGVELDYGSNELSPVLRGAIKGN
                     GNYLERILGELALGGDMRLLDEARPLVKPLLSRRLAKHYGGFATSQLRAFDDKPTAKR
                     ALYVLRTAATGRHAMAHGEIVTDVARLGAFVPAEIGELLAIKQRGERQELDAEHAKVW
                     RTRLVTAIDAIDTAWPSSVLPIDPPPAAVEAADQWLRGIRKAHW"
     gene            complement(6251..6934)
                     /locus_tag="VFV99_06485"
     CDS             complement(6251..6934)
                     /locus_tag="VFV99_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5058987.1"
                     /translation="MKRFALGLLCVSSSAFAGSIKGTVLYEGEPPTQETLKRDSDPKC
                     SKDKTDEAVVVTKGKLRDVVVRIKNGTAGKHAAPTTPVLLDQKDCMYVPRVVGIVAGQ
                     KLLVRNSDNTFHNVWGQVANKDAFNKPQAPKAADLTLDPSSAKADDVVELKCGVHGWM
                     HAYVAVQDHPYFAVTAADGTFEIKGLPQGKYTLEAWHPTLGTKSMQVEIGKGKRADVT
                     ARFSYKQTD"
     gene            6977..8761
                     /locus_tag="VFV99_06490"
     CDS             6977..8761
                     /locus_tag="VFV99_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058988.1"
                     /translation="MVDLPVKLGTMDAVPPGLRYRIKDVLGLLPIQGGSLDGVARERL
                     TRLIEDGEPWLGADKSLAQVGYWMLDAKHAVELRKQGCIWLTMFPSVETVARLATIAR
                     DAATPPLVREQAIWTLGYRQAQALHPTTQWPAEAVQLADEALVKLADTATTAGKITSE
                     ELPFAMRHVQWEGASAVFARAPGLWGEAIECFATPALARVLLVCLEDIPPQHRLRAIR
                     LVGAVLGEEAVPMLLARAAQAAIDEKLEMLFMVVSLAGEAKLGLLEDALKGMKYVDLL
                     RQRAKWHLQHSRVVPTVRGLRVARTTGVMAPAERAKACAQAADDLGALTKFARHPEAY
                     LYTMWGWMVRGSADPVRARDLVAAHPESQRLVRDLYIEDLARRGRVKQLTAAAQTLQG
                     ADLGAMHLATFGRPIAALELAATARLHTPELVFARALACYRAGRPDLTDRILAEDMPP
                     AEIVNDDSLPPFPGPHEQWLIEHAPTARPAVVALAKGREAILAAAKGAPFDAEGDTPS
                     IDPIGAVVRRLGRGLRGSTVYLAGEFKYLDKGKLAKAVEAAGARLVNGPFPGTDYYVH
                     GDWCLVQTIAQLERQGARRLRSGELEGL"
     gene            8762..>10101
                     /locus_tag="VFV99_06495"
     CDS             8762..>10101
                     /locus_tag="VFV99_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058989.1"
                     /translation="MPPRTSSPVGSGAALPKPHRIAFLMPDVKIEGPEGAYEREAAVL
                     LWVACIETCQRHPRLAVLDADATPLFPQDGHFAPQHAGRGGRATDAFYGPTRRDEVMW
                     LELLISNKPGVVRLHVIGRDGKQETFDALGPKLGEQIQQVFGAWLTARGLGALPRRFD
                     HVTSDDVLGVLRVIAPTLAEQARAWQLPVATKPTWSLSIVEDETEEADPDDDPEPTGS
                     GESDDDEDDGADVDEFDRAFDRAVDSTIDSTLDLPASQAPPDSAPPQHMISRPLINRL
                     AMPFKLPALRLLELALREDLGDAILAVDPSHPQALFARFEKMAPNGKDFALLRQVIAS
                     APGWARPYEELQADEDEQDDHFAMARPGVPSDLESIAAAGMASLCRPAALHALSTIGD
                     RLADDGRVDEGVRLLERGVEMEDDDAGAHLALLDLHERTDRVGAWLEQSMRSASL"
BASE COUNT         1530 a         3402 c         3477 g         1692 t
ORIGIN      
        1 tggggtcgca ctcgtcgggt ggctgacacc aacggttgcc gagctcgtcg cacctggttc
       61 gcacgcgacg aagtggatcg acgcccacat caccgaggag atcgtcggtt tgttcgctgg
      121 ctgttcgttg ttcgtgctcc cgggcggcaa ggaccgtcct gcactcacgt ggcgcgaagc
      181 aacgcagatc gaatggggcg tgatcttgtt attcggcggt ggcatcttgc ttggcacgct
      241 cgcgaagagc accgggctcg ccgccgagtg gggccatgcg ctcgtcgatg cgaccggcgc
      301 atcgagcacg tggtcgatca ccgtgctcgt gaccggcgtc tcgatcgtgc tctccgaggc
      361 gacgagcaac accgcgaccg cgagcatgat ggcgccgctc gccggtgcgc tggcggctgc
      421 tgccggtgcc gcgccgattc ccgccgtgct cggcgcgacg atgggcgcgt cgttcggctt
      481 catcatgccg atctcgacgg caccgaacgc actcgcctac gcgagtggtc gtgtgagcat
      541 cggccagatg ctgcgcgtcg gcattgtgtt cgatgggatc ggttttctct tgatcgtcgg
      601 gtcactcaga gtcatgtgcc cgctcctcgg ctgggcgtga cgatctgccg cacctcggtg
      661 cggcgacctg gcgcatccca gttccatgga cgtgctcgtg atcgggggcg gcggtgcgct
      721 cggccgcctt gtttgcagcc agctcaccgc gcgcgggcat cgcgcactgg ccatcggtcg
      781 ccgcgacggc aacctccgcg acccggcatt gatcgcacgc gcgggtgcgc cgctgatcat
      841 caactgtgca ggtgcgagcg tgcagatggg tctcgggcac ggctggcgcg gctatcgcgc
      901 cgtcgatgtg ccgatcgggc ttgctgcggt cgaagcggcg cgacgtacga aggcgaggct
      961 cgtctacgtc ggcgtgcatc atccaccggc cctgcgggcg accgcgtacg tcgatgcgca
     1021 cgagcgggta gccgaggcga tggctgacat cgacggcgcc gtcgtgcgac cgaccgggtt
     1081 tttctcggcg atcagcggcg cgttcctgcc gatggcaaag cgcggccgca tgttcgacgt
     1141 cggtgacggc agcgcgcgca cgaatccgat ctgcgagcgc gatcttgcgg agatcgtcgt
     1201 cgatgtcgcg ctcggcggcg atggcccgcg cgatgtctcg gtcggcggtc cggaggtgat
     1261 gacccgtcgc gagatgctcg agcgcgtcgc gtcggccgcc ggctatcgcg ccaacgtcac
     1321 cggtatgccg ctcgcgctgt gcaagctcaa cgcagcgctg ttgcgcgccg tgcatccacg
     1381 gatgggccag ttcgtgcagt tcgtggtcgg gctcgcacaa cacgacgtga tcgcgccggc
     1441 ctgcggcacg acgaagctcg gcgactacgt cgcatcgttg ccgatcgcgc gcgcggctta
     1501 gtcgtcggtc tcgctgttcg tcgtcggcct acgaaccgcg gcgaacatac cggccttgcg
     1561 cacgggcggg tggctatcga ccgtaccctg cgcaagctcg acctccgcag gcagcggtgg
     1621 cgccggcggc cagcccaagt acgtgtcctc gcgtaccgcc gccacctcca ttcgctcggt
     1681 gtcgtggaat tcgtcgtcga gctgcggaat cgcgtactcg tcctcgacct gcgggacggg
     1741 cgtctccgcg tcgagctcct cgagcacgtc atatccggac tcgtcgtcca tacctggtct
     1801 tcaatcgtat cccgctggcg gacattccct gaagacaatc ggctgtgccg cgatgtgcgc
     1861 ttacaagccg aagcgtcggc gtagctcgtc ggaagtgccg tcgaacacat cgagatctac
     1921 ggcgccgtcg atgccatcga cgcggccatt ctcggtgtac tgccagaacg tccaccgctg
     1981 ccacggcggc gggatcgacg gacacgcttc ggtgtagtgc gcgagccaga gcaactggcg
     2041 cgcgagcggt tcggcaccgc cccagcggaa catgccgtag ttcgtgtaca cgatcggctc
     2101 gacgccgagc tgactgcgca cctgctcgat ccagacgcgc gcgttcgcga cgacggtcgc
     2161 gagcggcacg ccatcggtca cctcgatatc gatcacgggc ggtagctcgc cagcgccgcg
     2221 tgcgcgcagc gtgcgaacca gcaggttcgc ctgttcgatc gggctctcgt tcggccgaaa
     2281 gaactgatac gcgccgcgca gtacgtgtgc gcgcttcgcg ccggtccagt tcgcctcgaa
     2341 cttggtgtcg acgatcgtcg cgccatccga gacgcgaatg aacgcgaact gaacaccggc
     2401 acgcttcacg cgcgaccacg tgatgtcacc ctggtagtac gagacgtcga tgccggccgt
     2461 cgtcggcccg agcttgcact cgggcggcgt cggcttcttc ttgaccgccg ggtcgcggtt
     2521 ctgcgtcgcc tgcatgacga cgtaggcggt catcgcgacg agcgccacgg cgatggctac
     2581 gccaaaccgc acgctgcgct tacgttttcg caccactgag ctaaccgccg agtcctgcca
     2641 agtattcgat cacgtggcac catgatggtg acatggccct gcgcctgggt gggttagctg
     2701 acctcgatcc gagcgcggtt ccgctgccgg cgggaaccga ggtgacgacg cgcgtcgatc
     2761 gcgcggtcga cggcggcgag atgcgcccgc agggtgcgac gggcagggtt gccaagctcg
     2821 acggcgatcg cgtcgaggtc gtgttcctcg acggcaagcg cgcgacctat ctgcgcggcg
     2881 aggtcatgcc gcgcaagctc ggcgtcgtgc gctatgcgca gcgccgggcg gcggcgtggg
     2941 agctcctgca tccatgcgtt gcgatcgaca cggtcgtcgg ctcgcgcgcg tggggcgtcg
     3001 ccgacgcggg ctccgacgag gatcatcgcg gcgtgttcgt actgccgatg ccgtggacga
     3061 ccggtctcgt cgatccgccg ctcgatctcg tgtcggccga tggctcgtcg acctactggg
     3121 agctcggcaa ggcgatccgc caggcgctac gcgcggatcc gaatacgctc gagatgctgt
     3181 tcgcatcgcc ggcgatcgtc gatccgatcg gcgagccgct ggtggccgcg cgcagcgcgt
     3241 tcttgtcgca cgagatctac ggcagcttcg gccgctacgc gctgtcgcag ctcgaccgcc
     3301 tcgagcacaa ccagcggctc gccgagcatc gcgtcaccgt gatcggctgg ctgcgcgacg
     3361 atccggatct cgcgctcgac accgctgccg agcggcttgc cgatgctgcg cggatcgaag
     3421 cgccgtcgcg cgccgacgcg atcggtcgcg cacgcgacta catcaagcag ctctaccgct
     3481 cgatgtacga tcagggtttg atcgccgcga acgattgggc atcgctgcgc gccgtcgcgc
     3541 gcaccgacct cgagctgccg cgcgatctgc ggccgaagaa cgcgtacaac ctgatccggc
     3601 tgctcgatct cgcgatccgc tggctcgccg gcgaatcgcc cgacgtgcgc gtctccgacg
     3661 agctgcggcc aacgctcgtc gcgatcaagc gcggcgagct accaatgccc gacgtgatgg
     3721 cgctcgcgcg cgcgctcaca ccgaagctcg aggccgcgcg ccagaccagt ccgttgccac
     3781 gctaccccga tgtcgccgtt gccgatcgcg tgctgcgcgc cgcgcgggac gaagcggcgc
     3841 gacgttgggt ccagcgcgag cccgggccat ggggagctgc ggcgccaccg ccgccggagg
     3901 cgcactatga cgagtgacat cgtcatcctg tcgccgcacc tcgacgacgc cgtgctctcg
     3961 ctcggcggga tgatcgggcg cgagatcgcg gccggccgct cggtcgaggt cgtgtcgtgc
     4021 ttcaccgccg gtccgccgct cgacacgatc gcgccggcgc accgcgtgtt cggcgactac
     4081 tcgatgcgcc gcgccgaaga cgaacgcgcg ctcgccgtgc tcggtgcgcg gcaccgctgg
     4141 ctcgatctac acgagcgcat ctggcgccag ccgccactgc cgccgagcgg cgtgttcccg
     4201 acgcacgtgt tccgcacgcc cgagcgcatg gaagatttcg ccgagctcaa gacgatccgc
     4261 gccgcgattg ccgaggttct cgacggtggc gcgacgacgg tctacgcacc gctcgcgatc
     4321 ggtcaccacg tcgatcacgt cgaggtcgca ctcgccgcgc tgcgcgaagt gctcggccgc
     4381 ggcgcgttcg agcgcattca cttctacgaa gacccgtacg cgctcggccg cgcgtgccgc
     4441 aagcagcact tcgtcgcgaa gcgccggatg tggcggccgt tcggcgcgcc cgcatgggcg
     4501 agcccgcgcg tcggtggcct gctccgtatg gtcgcgttcg tcgcgaaagg gccgaagctc
     4561 gaagattacc tgcccgaggc cgatcgcctc gagtggacgt gcacgccgca cgagatcgcg
     4621 tttgccgacg aggagcgcaa gctcaccgcg atcagcgagt acgtctcgca ggtgcgcgcg
     4681 tttggcggca tggagcgcgt tgccgcgttc atgcgtcgcg cccacaaggt actcggcggc
     4741 gagccgctat ggtcgtgccg gccggcgatg ccgaggtagc gtcgcggcaa tagcgaacgc
     4801 ggccggggcg ttgcgcttcc atgaaggttt ggctgctcgc cgcgctgctc gtcgtctcca
     4861 ccgcgcacgc tgacacgttc gagcttcgtg acgtgccgag tacgacgaat cgcccgccat
     4921 tcgtcacgcg cacgcgcgac tgcaatccga atggcgtgtc gcagatgcgc acgttgaccg
     4981 cgaccattgg cggctacgag gtcgagctgc atcgcgactg catggcggcg ccccagatgt
     5041 catcgctcac agtacgcatc cacgaggact ggttcgtcgc cgagggtttc acgctcacgg
     5101 acttcggcgg caacatgagc gactcgcgga tcgagatcca tcgcgtgagt gaatcgctcg
     5161 tcaaagggac gttcgccgat ggctcgccgg cgatcgtgta ccgcgcggtc acccggaccg
     5221 acttcgtcag ctcatgcgac ggcgatgact gtgtcgatcc ggtcaccgag agcgtgaagc
     5281 acgacaactt cctcgtgtgc agcgtcgcca tggagaccgt ccggtgcggc cgcatcgagt
     5341 acgagtgtcc ggccacgggg tgcggtgcgc cggcgttcgt cctcggtcag ctcgtcgcga
     5401 tcgaacagcg cacgtacgtg ccggctcgcc gcaactgacc gcggtaagct catctcgatg
     5461 agcttcgatc cgtggagcgt gctgaccgcg aagcaggcga cgatcgcgcg cgcgttcctc
     5521 gccgaccgcg aacgcgagcg caagcacctc gtgatctatc tgtccggcgc gcacgcgtac
     5581 ggattcccgt cccccgatag cgatctcgat ctcaagtgcg tacacatcgc gccgacccac
     5641 gacctcgtcg gtctcgacgt cgtcgctgat ccaaacgatc gcatcgacat cgtcgacggt
     5701 gtcgagctcg attacggcag caacgagctg tcgccggtgc tccgcggcgc gatcaaaggc
     5761 aacggcaact acctcgagcg tatcctcggc gagctcgcgc tcggcggcga catgaggctg
     5821 ctcgacgaag cgcggcccct cgtgaaaccg ctgctctcac gccggctcgc caagcattac
     5881 ggtggcttcg cgacgagcca gttgcgcgcg ttcgacgaca agccgaccgc gaagcgcgcg
     5941 ctctatgtcc tgcgcaccgc agcgaccggc cgccacgcga tggcacacgg cgagatcgtg
     6001 accgacgtgg cgcggctcgg cgccttcgtg ccggccgaga tcggcgagct gctcgcgatc
     6061 aagcagcgcg gcgagcgcca ggagctcgac gcagagcacg cgaaggtgtg gcggacccgg
     6121 ctcgtgaccg cgatcgacgc gatcgatacc gcgtggccgt cgtcggttct tccgatcgat
     6181 ccaccacccg ctgcggtcga ggccgccgac cagtggctac gcggaatccg aaaagcgcac
     6241 tggtgagtcg ctaatcggtc tgcttgtacg agaaccgagc agtcacgtcg gcgcgcttgc
     6301 ccttgccgat ctcgacctgc atcgacttcg tgccgagcgt cgggtgccac gcctcgagcg
     6361 tgtacttgcc ctgcggtagg cctttgatct cgaacgtgcc gtcggcggcg gtcaccgcga
     6421 agtacggatg atcctggacc gcgacgtagg cgtgcatcca gccgtggacg ccgcacttga
     6481 gctcgacgac gtcgtcggcc ttcgcgctcg acgggtcgag tgtgaggtca gcggccttcg
     6541 gggcctgcgg cttgttaaag gcgtccttgt tcgcgacctg tccccacacg ttgtggaacg
     6601 tgttgtcgct gttgcggacg agcagcttct ggccggcgac gataccgacc acccgcggca
     6661 cgtacatgca gtccttctgg tcgaggagga ccggcgtggt cggtgcggca tgcttgccgg
     6721 ccgtaccgtt cttgatgcgg accaccacgt cgcgcagctt gcccttcgtg acgacgaccg
     6781 cctcgtcggt cttgtccttg gagcacttcg gatccgagtc gcgcttgagc gtctcctggg
     6841 tcggcggctc accctcgtac agcaccgtgc ccttgatgga gccggcgaag gcggaggagg
     6901 acacacacaa caaaccgagc gcgaaacgct tcatggatga tggcctgact atgacacggg
     6961 cgcttattct cgtgccgtgg tggatttgcc cgtgaagctg ggcacgatgg acgcagtccc
     7021 gccgggactg cgctatcgga tcaaggacgt cctcgggctg ctccctattc aaggcggctc
     7081 cttggatggc gttgcacggg agcggctgac ccggctcatc gaggatggcg agccgtggct
     7141 cggcgcagac aagtcgctcg cacaggtcgg ctactggatg ctcgacgcca agcacgcggt
     7201 cgagttgcgc aagcaaggct gcatctggct gacgatgttt ccgtcggtcg agacggtggc
     7261 ccggctcgcg acgatcgccc gagatgccgc aaccccgccg ctggtgcgcg agcaggcgat
     7321 ctggacgctc ggctaccggc aagcacaggc gctgcatccg acgacgcagt ggccggccga
     7381 ggccgtgcag cttgccgatg aagcgctcgt gaagctcgcc gacacggcga cgaccgcggg
     7441 caagatcacg agcgaggagc tgccgttcgc gatgcgccac gtgcagtggg aaggcgcgtc
     7501 ggcagtgttc gcgcgcgcac ccggcttgtg gggagaggcg atcgagtgtt tcgcgacacc
     7561 ggcactcgcg cgcgtgttgc tcgtctgtct cgaggacatt ccgccgcagc accggctgcg
     7621 tgcgatccgg ctggtcggtg cggtgctcgg cgaagaagcg gtgccgatgc tgctcgcgcg
     7681 tgcggcgcag gcggcaatcg acgagaagct cgagatgctg ttcatggtcg tgtcgctcgc
     7741 tggcgaggcg aagctcggcc tgctcgagga cgccctgaaa ggcatgaagt acgtcgacct
     7801 gttgcgccag cgcgcgaagt ggcacctgca gcattcacgc gtggtgccga cggtgcgcgg
     7861 gctgcgcgtc gcacggacga ccggtgtgat ggcgccggcc gaacgcgcga aggcatgcgc
     7921 gcaggcggcc gacgatctcg gcgcgctgac gaagttcgcc cgccatcccg aggcatacct
     7981 ctacacgatg tggggctgga tggtgcgcgg ttccgcggat ccggtgcgcg cgcgcgacct
     8041 cgtggcggcg catcccgaat cgcagcggct cgtccgcgat ctgtacatcg aggacctcgc
     8101 gcggcgcggt cgcgtgaagc agctgaccgc cgcggcgcag acactgcaag gcgccgatct
     8161 aggcgcgatg cacctcgcga cgttcggcag gccgatcgcg gcgctcgagc tcgcagcgac
     8221 tgcgcgcctg cacacgcccg agctcgtgtt tgcccgcgcg ctcgcgtgct atcgcgcggg
     8281 caggccggac ctgacggatc gcatcctcgc cgaggacatg ccgccggcgg agatcgtcaa
     8341 cgacgacagc ctgcctccgt tccccggccc ccacgaacag tggctgatcg agcacgcgcc
     8401 caccgcgcgg ccggccgtcg tggcgctcgc gaaaggacgc gaagcgatcc tcgccgctgc
     8461 gaagggcgcg ccgttcgatg cggagggcga tacgccgtcg atcgatccga tcggcgcggt
     8521 cgtgcgccgg ctcggccgcg ggttgcgcgg ctcgacggtg tatctcgccg gcgagttcaa
     8581 gtacctcgac aagggcaagc tggccaaagc cgtcgaggcg gcgggtgcgc ggctcgtcaa
     8641 tggtccattc cccggaaccg attactacgt gcacggcgat tggtgcctcg tgcaaacgat
     8701 cgcgcagctc gaacgccagg gtgcgcggcg cctgcgttcc ggcgagctgg agggcctcta
     8761 ggtgccgccc aggacgtcat caccggtcgg ctcgggtgcg gcgctgccga aaccgcatcg
     8821 catcgcgttc ttgatgcccg acgtcaagat cgaaggcccc gagggcgcgt acgagcgcga
     8881 ggctgcggtg ctgctgtggg tcgcgtgcat cgagacgtgt cagcgtcatc cgcggctcgc
     8941 ggtactcgac gccgacgcaa cgccgctgtt tccgcaagac ggtcacttcg cgccgcagca
     9001 cgcaggacgc ggtggccgcg cgaccgatgc gttctacggg ccgacgcgtc gcgacgaagt
     9061 gatgtggctc gagctgctca tctcgaacaa gcctggcgtc gtgcgcttgc acgtcatcgg
     9121 tcgtgacggc aaacaagaga cgttcgatgc gctcggcccc aagctcggcg agcaaatcca
     9181 gcaggtgttc ggtgcgtggc tcaccgcgcg tggactcggc gcgctgccgc gccgcttcga
     9241 tcacgtgacg agcgacgacg tgctcggcgt gttgcgtgtg atcgcaccga cgctcgccga
     9301 gcaagcgcgc gcgtggcagc tgcccgtcgc gacgaagccg acctggagtc tctcgatcgt
     9361 cgaggacgag accgaagagg ccgatcccga cgatgatccc gagccgacgg ggagcggcga
     9421 gtccgacgac gacgaggatg acggcgcgga tgtcgacgaa ttcgatcgcg cgttcgatcg
     9481 cgcggtcgat tcgacgatcg actcgacact cgatctgccg gcatcgcaag caccgcccga
     9541 tagtgcgccg ccgcagcaca tgatctcgcg gccgctgatc aaccggttgg cgatgccgtt
     9601 caagctgcct gcgttgcgcc tgctcgagct cgcgctgcgc gaagatctcg gcgatgcgat
     9661 tctcgcggtc gatccgtcgc atccgcaggc actgttcgcg cggttcgaga agatggcgcc
     9721 gaacggcaag gacttcgcgc tgctccgtca ggtgatcgcg agcgcgccgg gttgggcgcg
     9781 cccgtacgag gagctccagg ccgacgagga cgagcaggac gatcacttcg caatggcgcg
     9841 gccgggcgtg ccgagtgatc tcgaatcgat cgctgccgcc ggtatggcgt cgctgtgccg
     9901 gcccgccgcg ttgcacgcgc tgtcgacgat cggcgaccga cttgccgacg acggtcgtgt
     9961 cgacgaaggc gtgcgattac tcgagcgcgg cgtcgagatg gaggacgacg atgcgggcgc
    10021 gcacctcgca ctgctcgatc tgcacgagcg caccgatcgc gtcggcgctt ggctcgagca
    10081 atcgatgcgt tcggcgagcc t
//
LOCUS       DASUFX010000163         8320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1693168, whole genome shotgun sequence.
ACCESSION   DASUFX010000163 DASUFX010000000
VERSION     DASUFX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693168"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="VFV99_06500"
     CDS             complement(<1..1970)
                     /locus_tag="VFV99_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5058990.1"
                     /translation="MPPLRWLAVLALCACGDNIFGDGGPLVPSDDLVIVAHQDDDLLF
                     MQPDVLDTVKRGGGLTSVYVTAGNGTSGTSSEERYAGLRAAYAEAAGSSNWECGWILV
                     LDHYAQHCRLADRPVSLVFLGYPDGGKTGESGDSLLHLWEGRVTRVETVGDHTTTYDR
                     SELVETVAQIIRATQPRHVHTLEVAATHGRDHSDHMLVGALALLAMARANSLADVLAY
                     RGYKIADEPPNKLPAVYDAAFAVLARYEACTSDCGECGGTCTAIDQTHVTWLLRRYAV
                     GFRNAAAGRLRIDTRCLGNDLTLADCATAPSWRFDPYGELRTSDGRCLAVDASGNLSL
                     DACLGGPRRRVFLDDEGHIWSGVPPVPTTNMDYAHLSCLTPTDTGPRMQLCGLDAAPA
                     WEVVPRTVATPRADIGFSATGRDVRLGDLTGDHHADLCTIDGGLGLLCAPGDGTGGFA
                     AAVRIDSITKPLTIDPKSLTLGDVDGDDRLDACGRDAEGILCATAASGFFAARWSPSF
                     NDDVARSGTSQSITAIDANDDGRADICGLDLSGVVCAPHGLTLQPLIRSAWPQPTSVV
                     WPADLDGDRQADWCSATDDGPMCAVEAERNVTTDGAPWGYASDGVVEVAPATTVTVSF
                     GDIDGDHRADQCSTREDRIVCARSQGRAFGPRT"
     gene            2013..4784
                     /locus_tag="VFV99_06505"
     CDS             2013..4784
                     /locus_tag="VFV99_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058991.1"
                     /translation="MDRLVRLARWFGANPAAQLVVGGLVLMVAFPGAPIGTIGRLVVY
                     GALGAAFALGWPAMPAAVRTPPPRIATAVALGVVALVGLSVFWPVLSQSPSPEWQTGD
                     WGPQHAVLARIMPHLPGLDIPVWNHAVSTGDAPLELYPAFTYIVTGHLALLFGLENDL
                     PHAFMIVATLTHLGLALTTVALAMRVASKPIAMIVGIFFLVDSGAISHGGTVGLFHWA
                     LLHSAFAHLFSMIAALGILAALRRPRLGASMTIWLGIAVSTAAHPAALITTATYCIAL
                     AMVALLASDVQPRRALAAMGHVVIGLALGATVWMPAAERLLEYGQHFPNELYTPDKLL
                     ELLGPYALPITAYSFIVYSGHLGTLFGMWTRRANVIFIAIIVLVMMLGLSDGPYLALG
                     LAPSKMVARLGAIRMMLLVRPFVFAAAAFTFHSLVRRAREAWVGAGERQRVIAAVLLA
                     IMIGCFSRVVPEYWTNESDRALGEANQYAPDLEGQNQLEQWAEQQAAQITPERWARAM
                     FEVTTHEHMHLTAKTGLPSFHLTPIPDLLLRERIEDASPESLARFNVRWVVGQGASPQ
                     LGDPATEKQFGIYHVREVKEWDGKFARIERGVGTVRVTRLDDEAVEIDVDAQAPVLVA
                     LGMGYYPRWRATHAGGANEPVFAMPTIQGGSLHVVAAWVAPGHTTFTCDGRLPSDGKG
                     RFISLLAALFAVAAIIVWRRARWRVRVLRRAIWLRDLVRARWQRVIEMAVPAVLIFLV
                     VVGAITQRQSATALLVGSSGIRPTAHVEARFVNNGEWETCGFSPFTGAYRCPDLVNVS
                     DATVNLMNDAPPSWAFITPAINAYAETAQVEIRITRTVRLGGRYWLGSSYGNVKLLMD
                     NSFAHEFGAKSTIDIPRGQHTIELTTQLPDDGSKSIVFVAERTLVPERTFLVEPPLQP
                     PASVSAIAK"
     gene            complement(4765..5700)
                     /locus_tag="VFV99_06510"
     CDS             complement(4765..5700)
                     /locus_tag="VFV99_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5058992.1"
                     /translation="MEAQRSAWQRYGWLLRWLGTAAGIAYIATLIKPDEVKDAFAKIS
                     IGAWVLATALVGLNVVIGAARWRALLSAYGALTRPDLWRATQLYFISFFYNNYLPGAV
                     AGDVVRGVITRDVFGERGATAGLAVVLVERALGLFGVFLLLAVGLLAAGGSLDTGSLW
                     LWSALGIAGSFALVLALPFARRLARFLPGKLRSIAERVPALSSTLPFIGAAVQSVLTQ
                     VCVAMAGYVLLHAIEPRVDLGSSLLIVPLAAATTFLPITVGGAGAREAVYITLGASLF
                     QMTRSDALAASLALWIAHLINGAIGGVVQLTSRSR"
     gene            5744..7126
                     /locus_tag="VFV99_06515"
     CDS             5744..7126
                     /locus_tag="VFV99_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058993.1"
                     /translation="MSFVSRVRALSPTTILAIGWLGLVLYAFPGHMSFDSAAQLIESR
                     GGSYTDAHPPAMAALWRLVEVFVSGPVGILLIQTTTLLAGAYLLLKRRMSPRAAAVTT
                     SLLLWFPVVSNTMSTIWKDSLMSAFLLLGTALLLSERRNVRIGGLAFLAAATAMRHNA
                     FAMTLPLVVLLFVWNPAYAWWRRYAIAFAAWIVVTGSAQFVNRALTRHELHLWHQSLA
                     ELDIVGTLQYVDDIPDAELHRILDGTGAVLTDHQHAKLRVARAPDVDVVAQLWVVTDA
                     LFARPRPPKLGLRWPDVQTPAQRDAMARAWKEVVPTHLSAYLHYRWDVFRQLMQMTEV
                     PVGSAAYLWFNDIQDIDDSAYKIGHDASYSGIQRPLHDAMWWLGSTVLFNVYVYVLLA
                     IALLPWCLRDREVLGLVASGLVGSAGLFVVAPTTDFRYMFWLVLCALLGLIMTVTRQI
                     AWVTASTMKQ"
     gene            complement(7087..>8320)
                     /locus_tag="VFV99_06520"
     CDS             complement(7087..>8320)
                     /locus_tag="VFV99_06520"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HEX5058994.1"
                     /translation="ATVLYAGVAGAFIGYLMIFFAPGQGQRYEGLAERYTLTQQILVR
                     GFAGNMDIFLDFLAASAPLLLLMVAAFVLGIVTDGGEDAAEQRDRQRKAFTFVGIALL
                     AGSLITITVFASPKLGPRFYMHGGALLLAATLGVLSISLRKTKTLVPFVVFAVIASIY
                     AGARTIPLYTKMSGVSDKRLADLAATKPGSVFTAASWYPVPETWWALGDDVRDQKKQE
                     MVARYFGLSRVVFRGGDQWKTLGVSDVKLVMDYEFDKPLCVDEIEHLDIKDYVGRDVG
                     AIHHAFLDAIAQIEIVSGERPRKVDLKVTFLGAQPPMPSQRVYVGRWREGVLEGYTAK
                     LRRAGRSKERMIVLGDKLKQDSWDMYLMRVGEQPKLLGTNRDTDKLKYVPWASGEYWT
                     FACKPDYCFIVLAVTHAI"
BASE COUNT         1309 a         2761 c         2842 g         1408 t
ORIGIN      
        1 gtgcgcgggc cgaacgcgcg gccctggctg cgcgcgcaca cgatccgatc ctcgcgtgtc
       61 gagcactgat cggcgcggtg gtcgccgtcg atgtcgccga acgagacggt cacggtggta
      121 gcgggcgcga cctcgacgac accgtcgctc gcgtagcccc acggcgcgcc gtcggtcgtg
      181 acgttgcgct cggcctcgac cgcgcacatc gggccgtcgt ccgtcgcgct gcaccaatcg
      241 gcctggcgat cgccgtcgag atcggcgggc cacacgaccg acgttggttg cggccaggcg
      301 gagcggatga gcggctgcag cgtgaggcca tgcggcgcgc agacgacgcc gctgagatcg
      361 aggccgcaga tgtcggcgcg accgtcgtcg ttcgcgtcga tcgcagtgat cgattgcgac
      421 gtgccggagc gtgcgacatc gtcgttgaac gacgggctcc agcgcgctgc aaagaagccg
      481 cttgccgcgg tcgcgcatag gatgccctca gcgtcgcggc cacaggcgtc caggcgatcg
      541 tcgccatcga catcgccgag cgtcaggctt ttcggatcga tcgtgagcgg cttcgtgatc
      601 gaatcgatgc gcaccgctgc cgcgaagccg ccggtgccat cgccgggcgc gcagagcagg
      661 ccgaggccgc cgtcgatcgt acagaggtcg gcgtggtgat cgcccgtgag atcgccgagg
      721 cggacatcgc ggcccgtcgc ggagaacccg atgtcggcgc gtggcgtcgc gaccgtgcgg
      781 ggcaccactt cccatgcagg cgcggcgtcg agcccgcata gctgcatgcg cgggccggtg
      841 tcggtcggcg tcaggcacga caggtgcgca tagtccatgt tcgtggtggg cacgggtgga
      901 acgcccgacc agatatggcc ttcgtcgtcg agaaacacgc ggcgtcgcgg accgccgaga
      961 cacgcgtcga gcgagaggtt accgctggcg tccacggcga gacagcggcc atccgacgtg
     1021 cgcagctcgc cgtacggatc gaagcgccag ctcggtgcgg tcgcgcaatc ggcaagtgtg
     1081 aggtcgttgc cgaggcagcg cgtgtcgatg cggagccggc cggccgcggc gttgcgaaag
     1141 ccgaccgcgt agcgacgcaa cagccaggtc acatgcgtct gatcgatcgc ggtgcacgtg
     1201 ccgccgcact cgccacagtc gctcgtgcat gcctcgtagc gcgcgagcac tgcaaacgca
     1261 gcgtcgtaga ctgctggcag cttgttcggt ggctcgtcgg cgatcttgta accgcgatac
     1321 gcgaggacgt cggccaggct gttcgcgcgc gccatcgcga gcagtgcgag tgcaccgacg
     1381 agcatgtgat cggagtggtc gcggccatgc gttgccgcga cctcgagcgt gtggacgtga
     1441 cgcggctggg tcgcgcgaat gatctgcgcg acggtctcga cgagctcgct gcgatcgtag
     1501 gtcgtggtgt ggtcgccgac ggtctcgacg cgcgtgactc tgccttccca caagtgcagc
     1561 aagctgtcgc cgctctcgcc cgtcttgccg ccatcgggat agccgaggaa cacgagcgag
     1621 accggacgat ccgcgagccg gcagtgctgc gcgtaatgat cgagcaccag gatccagccg
     1681 cactcccagt tgctgctgcc cgcggcttcg gcgtaggcgg cgcgcagacc tgcatagcgc
     1741 tcctcgctgc tggtgccgct ggtgccgttg ccggcagtga cgtagacgct ggtgagcccg
     1801 ccgccgcgct tgacggtgtc gagcacgtcg ggctgcatga agaggaggtc gtcgtcctgg
     1861 tgcgcgacga tcacgaggtc gtccgagggg acgagcgggc cgccgtctcc gaatatgttg
     1921 tcgccgcatg cgcagagcgc caggacggcg agccaacgca agggcggcat cggcgcattg
     1981 tgcagcattt cggcgcgtgg tacgcgggga gcgtggaccg cctggttcgg ctagcgcgtt
     2041 ggttcggcgc gaacccagca gcgcaactcg tggttggcgg cctcgtgctc atggtcgcgt
     2101 ttcccggcgc gccgatcggg acgatcgggc gtctcgtcgt gtacggcgcg ctcggcgccg
     2161 cgttcgcact cggttggccg gcgatgcccg ccgccgtgcg cacgcctccg ccgcgcatcg
     2221 cgaccgcggt ggcgctcggt gtcgtcgcac tcgtggggct gagcgtgttc tggccggtgc
     2281 tctcgcagtc gccgtcgccc gaatggcaga ccggtgattg gggcccgcag cacgcggtgc
     2341 tcgcgcggat catgccgcac ctgccgggcc tcgacatccc ggtgtggaat cacgcggtgt
     2401 cgacgggcga cgcgccgctc gagctctacc ccgcgttcac gtacatcgtg accgggcacc
     2461 tcgcgctgtt gttcgggctc gagaacgatc tgccgcatgc gttcatgatc gtcgcgacgc
     2521 tcacccacct cgggctcgcg ctgacgacgg tcgcgctcgc gatgcgcgtg gcctcgaagc
     2581 cgatcgcgat gatcgtcggc atcttcttcc tcgtcgacag cggcgcgatc tcgcacggtg
     2641 gaacagtggg cctgttccac tgggcgctgc tgcacagcgc gttcgcgcac ctgttctcga
     2701 tgatcgccgc gctcggcatc ctcgcggcac tgcgacgacc gcggctcggc gcgtcgatga
     2761 cgatctggct tggcatcgcg gtcagcacgg cggcgcatcc ggcggcgctg atcacgacgg
     2821 cgacgtactg catcgcactc gcgatggtcg cgttgctcgc gagcgacgtg cagccgcgac
     2881 gcgcgcttgc cgcgatggga cacgtcgtga tcggcctcgc gttgggtgcg acggtgtgga
     2941 tgccggcggc cgagcggttg ctcgagtacg gccagcactt cccgaacgag ctctacacgc
     3001 ccgacaagct gctcgagctg ctcggtccgt atgcgctacc gatcacggcg tactcgttca
     3061 tcgtctacag cggccacctc ggaacgctgt tcggcatgtg gacccgacgg gcgaacgtga
     3121 tcttcatcgc gatcatcgtg ctcgtgatga tgctcggtct ctccgatggg ccgtacctcg
     3181 cgcttggcct cgcgccgagc aagatggtcg cgcgcctcgg cgcgatccgc atgatgctgc
     3241 tcgtgcggcc gttcgtcttc gcggcggccg cattcacgtt ccactcgctc gtgcggcgtg
     3301 cgcgcgaggc gtgggtgggt gcgggtgagc ggcagcgcgt gatcgcagca gtgctgctcg
     3361 cgatcatgat cggttgtttc tcgcgcgtcg ttcccgagta ctggacaaac gagagcgacc
     3421 gggcgctagg cgaggccaat cagtacgcgc cggatctcga gggtcagaac cagctcgagc
     3481 agtgggccga gcaacaggcc gcgcagatca cgcccgagcg gtgggcgcgc gcgatgttcg
     3541 aggtgacgac gcacgagcac atgcacctga ccgcgaagac cggtttgccg tcgttccacc
     3601 tcacgcccat cccggacttg ctgcttcgcg agcgcatcga ggacgcgtcg ccagagagcc
     3661 tcgcgcgctt caacgtgcgc tgggtggtcg ggcagggcgc gtcgccgcag ctcggcgatc
     3721 cggcgaccga gaaacagttc ggcatctatc acgtgcgcga ggtgaaggag tgggacggca
     3781 agttcgcgcg catcgagcgc ggcgtcggca ccgtgcgggt cacgcggctc gacgacgaag
     3841 cggtcgagat cgacgtcgat gcgcaggcac cggtgctcgt cgcgctcggc atgggctact
     3901 acccgcgctg gcgagcgacg cacgcgggcg gtgccaacga acccgtgttc gcgatgccga
     3961 cgatccaagg cggcagcctc cacgtcgttg ctgcgtgggt cgcgccgggt catacgacgt
     4021 tcacgtgcga cggtcgctta ccgtcggatg gtaagggtcg tttcatctca ttgctcgccg
     4081 cattgttcgc agtggcggcg atcatcgtgt ggcgtcgcgc acgctggcgc gttcgcgtgc
     4141 tgcgccgtgc gatctggctg cgcgatctgg tgcgtgcgcg gtggcagcgt gtgatcgaga
     4201 tggcggtgcc cgcggttctg atcttcctcg tcgttgtcgg tgcgatcacg cagcggcagt
     4261 ccgcgaccgc gctgctcgtc ggctcgtcgg gcattcggcc gaccgcgcac gtcgaggcgc
     4321 gcttcgtgaa caatggcgag tgggagacct gcggcttctc gccgttcacg ggggcgtatc
     4381 gctgccccga tctcgtcaac gtcagcgacg cgaccgtaaa tctcatgaac gatgcgccgc
     4441 cgagctgggc attcatcacg cccgcgatca acgcgtacgc ggagaccgcg caggtcgaga
     4501 ttcgcatcac gcgcacggtg cggctcggcg ggcgctactg gctcggctcg agctacggca
     4561 acgtcaagct gctgatggac aactcgtttg cgcacgagtt cggtgcgaag agcacgatcg
     4621 acatcccgcg cggccagcac acgatcgagc tgaccacgca gcttcccgac gacggctcga
     4681 agagcatcgt gttcgtcgcc gagcgtacac ttgtgccgga gcgcacgttc ctcgtcgagc
     4741 cgcctctgca gccgccggcg agcgtcagcg cgatcgcgaa gtgagctgga cgacgccgcc
     4801 gatcgcgccg ttgatcaggt gcgcgatcca cagcgcgagt gacgcagcga gggcatccga
     4861 ccgcgtcatc tggaacaagc tcgcgccgag cgtgatgtag accgcctcgc gggcaccggc
     4921 gccgccgacc gtgatcggaa ggaacgtcgt cgccgcggcg agcgggacga tcagcagcga
     4981 cgagccgagg tcgacacgcg gttcgatcgc atgcagcagc acatagcccg ccatcgcgac
     5041 gcagacctgg gtgagcaccg attgcaccgc cgcgccgatg aacggcagcg tcgacgacag
     5101 cgccggcacg cgctccgcga tcgagcgcag cttgccgggc agaaaccgcg cgagccgccg
     5161 cgcgaacggc agcgcgagca cgagtgcgaa cgaaccggcg atcccgagcg cactccacag
     5221 ccacagcgag ccggtgtcga gcgaaccgcc tgcggcgagg agaccgaccg cgagcagcaa
     5281 gaacacgccg aacaagccga gcgcgcgctc gacgagcacg accgcgaggc cggcggttgc
     5341 accgcgctcg ccgaacacgt cgcgcgtgat cacgccgcgt acgacatcgc ccgcgaccgc
     5401 gccaggcagg taattgttgt agaagaacga gatgaagtag agctgcgtcg cgcgccacag
     5461 atccgggcgg gtgagcgcgc catatgcgga caacagcgcc cgccaccgcg ctgcaccgat
     5521 cacgacattg aggccgacga gcgcggtcgc gaggacccat gcgccgatgg agatcttcgc
     5581 gaacgcgtct ttgacctcgt ccggtttgat cagcgtcgcg atgtacgcga tgccggcggc
     5641 ggttccgagc cagcgcagca gccagccgta gcgttgccac gcggaacgtt gcgcctccat
     5701 ggacggcgca gcatgccacg aatgtgcgta tagtcgcgca gctgtgtcgt tcgtgagccg
     5761 ggtgcgcgcg ctttcgccga cgacgatcct ggcgatcggt tggctggggt tggttctcta
     5821 cgcgttcccc ggccacatga gcttcgactc cgcggcgcag ttgatcgagt cgcgcggcgg
     5881 ctcctacacc gatgctcatc cacccgcgat ggcggcgctg tggcgcctcg tcgaggtatt
     5941 cgtcagtggc cccgtcggga tccttctgat ccagaccacg acgctgctgg ccggcgcgta
     6001 tctgttactc aagcgccgaa tgtcgccgcg agcggccgcg gtgaccacga gcctgctgtt
     6061 gtggttcccg gtcgtcagca acacgatgtc gacgatctgg aaagactcgc tgatgtcggc
     6121 attcttgctg ctcggcacag cgctgctgct atcggagcgc cgcaacgttc gcatcggcgg
     6181 gctcgcgttc ctcgctgcag cgaccgcgat gcgccacaac gcatttgcga tgacgctgcc
     6241 gctcgtcgtg ctgctgttcg tgtggaatcc ggcgtatgcg tggtggcggc ggtacgcgat
     6301 cgcatttgcg gcgtggatcg tcgtgaccgg cagcgcgcaa ttcgtcaatc gcgcgctcac
     6361 gcgccacgag ctgcacctct ggcaccagag tctcgccgag ctcgacatcg tcggcacgct
     6421 gcaatacgtc gacgatattc cggacgccga gctgcatcgt atcctcgacg gcaccggcgc
     6481 ggtcctcacc gatcaccagc acgcaaagct acgcgtcgcc cgcgcgcccg atgtcgacgt
     6541 cgtcgcgcag ctctgggtgg tcaccgacgc gctgttcgcg cggccgcgcc cgccgaagct
     6601 tggcttgcgc tggcccgacg tccagacgcc ggcgcagcgc gatgcgatgg cacgggcgtg
     6661 gaaggaggtc gtgccgacgc atctgtcggc ctatcttcat taccgctggg acgtgttccg
     6721 ccagctcatg cagatgaccg aagtgccggt cggctctgcc gcgtatctat ggttcaacga
     6781 catccaggat atcgacgaca gcgcatacaa gatcgggcac gacgcctcgt acagcgggat
     6841 ccagcgacca cttcacgacg cgatgtggtg gctcggctcg accgtgctgt tcaatgtcta
     6901 cgtctacgtg ctgctcgcca ttgcgctgtt gccgtggtgc ttgcgcgatc gcgaggtgct
     6961 cgggctcgtg gcgtcgggac tcgtcggctc ggctggcctg ttcgtcgtcg cgccgacgac
     7021 ggactttcgc tacatgttct ggctagtgct gtgcgcacta ctcggcctga tcatgaccgt
     7081 cacacgtcag atcgcgtggg tgaccgcgag cacgatgaag cagtagtccg gcttgcacgc
     7141 gaacgtccag tactcgcccg atgcccacgg cacgtacttc aacttatcgg tatcgcgatt
     7201 cgtgccgagt agcttcggct gctcgccgac gcgcatgagg tacatgtccc acgaatcctg
     7261 cttcagcttg tcgcccagca cgatcatgcg ctccttgctg cgaccggcac gcctgagctt
     7321 cgccgtgtag ccttcgagca caccctcgcg ccaccgcccg acgtacacgc gctggctcgg
     7381 catcggtggt tgcgcgccga ggaaggtgac cttgagatcg accttgcgcg gcctttcgcc
     7441 gctcacgatc tcgatctgcg cgatcgcatc gaggaacgca tgatgaatcg cgccgacatc
     7501 acggccgacg taatccttga tgtcgagatg ctcgatctcg tcgacgcaca gcggcttgtc
     7561 gaactcgtag tccatcacga gcttgacgtc gctcacgccg agcgtcttcc actgatcgcc
     7621 gccgcggaac acgacgcgag acagcccgaa gtacctggca accatctcct gcttcttctg
     7681 gtcgcgcacg tcgtcgccga gtgcccacca cgtctccggc accgggtacc acgacgccgc
     7741 tgtgaagacg ctaccgggtt tcgtcgcggc gagatcggcg agtcgcttgt cggagacgcc
     7801 cgacatcttc gtgtagagcg gtatcgtgcg cgcgcctgcg tagatactcg cgatcaccgc
     7861 aaacacgacg aacggtacga gtgtcttcgt cttgcgcagc gaaatcgaca acacgccgag
     7921 cgttgccgcg agcaacagcg ctccgccgtg catgtagaag cgcgggccga gctttggtga
     7981 tgcgaacacc gttatcgtga tcaaggaacc tgcgagcagc gcgatgccga caaacgtgaa
     8041 cgccttgcgc tggcggtcgc gttgctcagc ggcgtcctcc ccgccgtcgg tgacgatgcc
     8101 gagcacgaac gcggcgacca tcaagagcag cagcggtgcc gatgcggcga ggaagtcgag
     8161 aaagatgtcc atgttcccgg cgaagccgcg caccaggatc tgctgcgtga gcgtgtagcg
     8221 ttcggcgaga ccttcgtaac gctggccctg acctggcgcg aagaagatca tcaagtagcc
     8281 gatgaacgcg ccggcaacgc ccgcatacaa gaccgtcgcg
//
LOCUS       DASUFX010000164         9533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1971513, whole genome shotgun sequence.
ACCESSION   DASUFX010000164 DASUFX010000000
VERSION     DASUFX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971513"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFV99_06525"
     CDS             complement(<1..996)
                     /locus_tag="VFV99_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5058995.1"
                     /translation="MTKWLVLVLAVSACAAQGASSAGGDDSSGSGGGGVSFGGQQDIG
                     EFRGILESGGVPGKDTLDANGFFNEHYSEPPKSDCTSALCMTPGVSVGRDWLTGAHQA
                     TLQIAVNTNVDPTTITRKPLNLVVVVDHSGSMWDDQRLEKVKVGLHTLVDNLADGDRL
                     ALVQFDDRVDVLAPFSETPNKDLLHTLITNLTPRGSTDIYDGLATGLELASSAFSSDR
                     QNRVIFLSDGMATAGNTDPNAIIAMTDGYIEQGIGVTTIGVGLSFDVKLMRGLAEHGA
                     GNFYFLEDAAAATEVFNQELDYFVTPLALDVQIKATAGSGWKFGEVIGSSMWTSTP"
     gene            1154..2938
                     /gene="ggt"
                     /locus_tag="VFV99_06530"
     CDS             1154..2938
                     /gene="ggt"
                     /locus_tag="VFV99_06530"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5058996.1"
                     /translation="MRRLLLVVLVLGLVNCRQSQPTPEQQPRQAPVVVAKDAAVVVDA
                     GPPPPKFRTEASTFDVDPAYAGTGKTFMVASEDQLASQTGRDVLAAGGNAVDAAVAVA
                     FTLAVTRPSAGNLGGGGFAVVRTGKGKASTIDFRETAPAAATPTMYDDAGPKASLAGA
                     KAVGVPGSVAGLWELHKKYGTKKWAELIAPAIKIAKDGYPVTPLLAMGIKNRVKGLGM
                     TGALADRFAPGGTPLEVGATVKNPELAVVLERIAAKGPDGFYKGETAAAIVDVMKKEG
                     GLITEKDLAGYKAVWREPIKLTYRGKTLMTMPPPSSGGVVLAMTANMLRKVDLAKAGW
                     HSTEHLHQVVEVWRRAFAARNEVLGDPKFVKDMPVAKLTSQAEADRLAATITDRATPS
                     KDVPALLGGTHTTNFCVVDKKGMAVALTTTLNTSFGSGVMAEGVLLNNEMDDFATKPG
                     QPNVYGLVQGKANQIEPGKRMLSSMTPTIVEDEKGELYMVVGGQGGPRIITEVWQAIS
                     NVIDFGMTPDAAIMAPRFHHQHLPDEVVVEAEAITRDVDEALRGLGYTIVWNQPERIF
                     GAANMIVRSATGWAGSADRRGGGAALGD"
     gene            2968..3864
                     /locus_tag="VFV99_06535"
     CDS             2968..3864
                     /locus_tag="VFV99_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5058997.1"
                     /translation="MSGQGEPSGSVVYPKLPVPSNRNASEAKVKGFNQDDGYSKPKIA
                     AAVALVAAIGGAVGYLLAPDKGKELAAAKKEAAAQQTAAKTEKDRADGIAKQVDVLTK
                     DKTELEKQLSDMTAKAGEMEKKAQAANEAAQKKLKNAIGDEDGSVSTEGEEIHLKLVD
                     KVLFAVGDDQLTDKGKTVLDKVAKALKDIPDKQIWVQGHTDDSPIIVPPPPKKDPKKK
                     GKDKEPELVPFRFKSNWELSAARALQVVHYLQDHAKVDPTKLAALAFGEYRPVAKNNK
                     AANRRIEIVLYPHRAVIERAKK"
     gene            complement(3889..4674)
                     /locus_tag="VFV99_06540"
     CDS             complement(3889..4674)
                     /locus_tag="VFV99_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5058998.1"
                     /translation="MTGAAAKGGRILVVDDEPMVRETLGQVLAEEGYVVDVAVDGEDA
                     LDRVHAARPDVILLDLMMPGMNGRQFLQALRNDATYVNVPVLIMTAVHGLEVNLSSIG
                     ASEVVEKPFDVEDLLNKVALAVYRSRDADRLTHGPYVTNPTEPLPEPSDRGVVLIVER
                     DRSRLQQLDLVLSERGYTVVSMTRALAPLSRLARALRPRAILLELTSEGVHEVVRELR
                     SGRELEPMPVLLFARDGASGAGELDALEHAADSDLVSFVERQR"
     gene            4728..5198
                     /locus_tag="VFV99_06545"
     CDS             4728..5198
                     /locus_tag="VFV99_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5058999.1"
                     /translation="MSTAPSVARAYDGLRTKILQRILDMRATIDPSLGRVDEATARTQ
                     IGAVLDHIGNFIATGDLGLHRAFLHTFLAMRAAEAQGPAQVLAMLVAIGDTAAQVSQE
                     ESQGAEGSELTLLLTRVTAATARAVNDLIADELERRLAQWAELSTKEREALKPS"
     gene            5195..7039
                     /locus_tag="VFV99_06550"
     CDS             5195..7039
                     /locus_tag="VFV99_06550"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5059000.1"
                     /translation="MSTAVAPTLKVAGDTHVGKVRATNEDSMICDPVRGLYVVLDGMG
                     GANAGDIASQTARDAIRDFVTQRRLTMEPRALLEAAIQYGSAAVFAAAQAARERHGMG
                     TTVVACLVIDTKRVIIAHVGDSRAYLLRHGRLQSLTRDHTIVEELVDRGVLAAEEAER
                     HPYKNVLSRNLGAKAETRVDVLELDMKPGDRLLLCSDGLYGYASTEGMQYLLGSGDPP
                     EVVARDLIDLALRGGGGDNVSVIVLEAPQLAPTSTQVVRTSGATAWWQMRQRFLQVAK
                     ERGITKNPICRGLQPDEALDLVALSLCQAIFHDLEKSTGVNVWTFAQNIAGGWFERGG
                     EWSALRSLMDILAMSARTVVDEIRAADAKLGFLLDVAVSRALVVAELALGGLLAERLR
                     QVDSDLIAVHNAIQDTIDAEVDDVMSDVRFTEQATIPFLRPDRPVTHPGISAELVSGI
                     EKTLTIARARTSPRAELVKQILSSLETIATDTAGNFSAAVLAARELYGVRTVDDAGIM
                     SLFDALDSARIITASSVHQTSFPDALRAKVLGAISSAHQRLVGAMTGLVLEAVAPVTE
                     KLKAAQAVTAELRDKVGRAERHRAELAEKFATTIDQSLPWEKRGVTEW"
     gene            7041..7526
                     /locus_tag="VFV99_06555"
     CDS             7041..7526
                     /locus_tag="VFV99_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059001.1"
                     /translation="MSDVDLVEAVSVLRDEVLDTVEHGDRDPPGAEVFDALIRALTIG
                     GESVPGLDLTLHDSVTRRLAWGDSEEVVLADAEMVFDRLMVAVERALRDPADRMVVIE
                     AATQVAVTVARVVSLAAVGRASRDRAARLREEMAQRQLNEVLDKQRASIVNLERETRD
                     R"
     gene            complement(7523..7732)
                     /locus_tag="VFV99_06560"
     CDS             complement(7523..7732)
                     /locus_tag="VFV99_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059002.1"
                     /translation="MGLPELESYSIGEFVTYKLTKDPSSGTFTITLTGELKHALLIAH
                     KVANLTISEAELAELDVPLPTTATS"
     gene            complement(7835..8518)
                     /locus_tag="VFV99_06565"
     CDS             complement(7835..8518)
                     /locus_tag="VFV99_06565"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5059003.1"
                     /translation="MSRRAPRYRISLNVTVCPLLGGKMAVCHTRDVSEVGICLDTSEV
                     SEWLPVGTRVSLTMTDPRSGTAMEVIGDVVREVAGTAPALGVLLIEPPPEWRALVAAA
                     ARQSGTIEKPGRRMRVLVVGDDHRQRGAMALYVSSGWDVLFASDDYAVHEALEHIKLD
                     AVIAELDAADPRIEPIMDDVRKAQPAARRIVRGTGRTNTDLVHRYVDRDSGLEALLDA
                     ITANITPSA"
     gene            8634..9299
                     /locus_tag="VFV99_06570"
     CDS             8634..9299
                     /locus_tag="VFV99_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059004.1"
                     /translation="MRVALAIAALIAAPLTAAADEPAPSAPSAPPPTAGLPGMPTPTP
                     TPAPTGGAPGDTPSTTAPAPAPAPAPAGDAPDPAYDTRNFPAPKGADVVIVSYGERSR
                     KNVLMLGGIAAAGVVFGAVGLYFHLDSRSASNEVSSNKPTGEPWTPERADTYDRVHSS
                     SVAAGVFYGIGGALLLTSAVMYIVTEPKEEKTVIHPHYDPKPTALVAPTRGGAMIGGA
                     WRF"
     gene            9299..>9533
                     /locus_tag="VFV99_06575"
     CDS             9299..>9533
                     /locus_tag="VFV99_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059005.1"
                     /translation="MRALLLCIPLAACTPDVVSGSYLCGPDELCPDGQKCNGTEDKTT
                     GLAADSCVIPSLARPFACTPKVDVEPDDSMAQAH"
BASE COUNT         1689 a         3144 c         3190 g         1510 t
ORIGIN      
        1 cggggtcgag gtccacatcg acgagccgat cacctcgccg aacttccacc cgctgccggc
       61 cgtcgccttg atctggacgt cgagcgcgag cggcgtgacg aagtagtcga gctcctggtt
      121 gaacacctcg gtcgctgccg ccgcgtcttc gaggaagtag aagttgcctg cgccgtgctc
      181 ggcaaggccg cgcatgagct tgacgtcgaa cgagagaccg acgccgatcg tggtgacgcc
      241 gatgccttgc tcgatgtagc catcggtcat cgcgatgatc gcgttcggat cggtgttgcc
      301 ggccgtcgcc atgccgtccg acaggaagat gacgcggttc tggcgatcgc tggagaacgc
      361 cgacgatgcg agctcgaggc cggtcgcgag gccgtcgtag atgtccgtcg agccgcgcgg
      421 cgtgaggttc gtgatgagtg tgtggagcaa atctttgttc ggcgtttccg agaacggcgc
      481 gagcacgtcg acgcgatcgt cgaactgcac gagcgcgaga cgatcgccgt ccgcgagatt
      541 gtcgacgagc gtgtggaggc cgaccttgac cttctcgagc cgctggtcgt cccacatcga
      601 accggagtga tcgacgacga cgacgaggtt gagcggtttg cgcgtgatcg tggtgggatc
      661 gacgttcgtg ttgaccgcga tctgcaacgt cgcttgatgc gcgccggtga gccagtcgcg
      721 gccgaccgac acgccgggcg tcatgcacag cgcgctcgtg caatcgctct tcggcggctc
      781 gctgtagtgc tcgttgaaga agccgttcgc gtcgagcgtg tctttacctg gcacgccgcc
      841 gctttcgagg atgccgcgaa actcgccgat gtcctgctgg ccgccgaagc tgacgccacc
      901 gccgccgctg ccactgctgt cgtcgccgcc ggcggaactg gcgccctgcg cggcgcacgc
      961 ggacacggcg aggacgagaa cgagccattt cgtcatgcgg tcctgttcag caacgcgggt
     1021 gccagagccg gtttctgcga tttctgtgaa ccatcgcgtg cagttgcagc gcgcgacatg
     1081 ggcagccgcg ccgatcgcga gatccggcgc gaaccggcaa gtcacgacat cggtgtcgta
     1141 ggatgcgccg gtgatgcggc gcctccttct ggtcgtcctg gtcctcggtc ttgtcaattg
     1201 ccggcaatcg cagcccacgc cggagcagca gcctcggcag gcaccggtcg tcgtcgcgaa
     1261 ggatgcggcg gtcgtcgtcg atgccggtcc accgccgccg aagttccgta cggaagcgtc
     1321 gacgttcgac gtcgatcccg cgtacgccgg caccggcaag acgttcatgg tcgcgtcgga
     1381 agaccagctc gcgagtcaga ccgggcgcga cgtgctcgcg gcgggcggca atgcggtcga
     1441 tgcagcggtc gccgtcgcgt tcacgctcgc cgtgacgcgg ccgagtgcgg gcaatctcgg
     1501 cggcggcggc ttcgcggtcg tgcgtaccgg caagggcaaa gcgagcacga tcgattttcg
     1561 cgagaccgcg cccgcggcag cgacgccgac gatgtacgac gacgcgggac cgaaggcgtc
     1621 gctcgccggt gcgaaggcgg tcggggtgcc cggcagcgtt gccggcttgt gggagctgca
     1681 caagaagtac ggcacgaaga agtgggccga gctgatcgcg ccggcgatca agatcgcgaa
     1741 ggacggctac ccggtcacgc cactgctcgc gatggggatc aagaatcgcg tgaaggggct
     1801 cggcatgacc ggtgcgttgg ctgaccgttt cgcgcccggc ggcacgccac tcgaggtggg
     1861 cgcgacggtg aagaaccccg agcttgcggt cgtgctcgag cggatcgcag caaagggacc
     1921 cgatggcttc tacaaaggcg agaccgccgc cgcgatcgtc gacgtgatga agaaggaagg
     1981 cggcctgatc accgagaagg atctcgcggg ctacaaggcc gtgtggcgcg aaccgatcaa
     2041 gctcacatat cgcggcaaga cgctgatgac gatgccgccg ccgtcgtcgg gtggtgtcgt
     2101 gctcgcgatg acggcgaaca tgctgcgcaa ggtcgacctc gccaaagccg gctggcactc
     2161 caccgagcat ctgcatcaag tcgtcgaggt gtggcgacgg gcattcgctg cgcgcaacga
     2221 agtgctcggc gatccgaagt tcgtcaagga catgccggtc gcgaagctga cgtcgcaagc
     2281 ggaggccgac cggctcgctg cgacgatcac ggatcgcgcg acgccgtcga aggacgtgcc
     2341 ggcgctactc ggcggcacgc acacgacgaa cttctgcgtc gtcgacaaga aaggcatggc
     2401 ggtcgcgttg acgacgacgc tcaacacgtc gttcggcagt ggcgtgatgg ccgagggcgt
     2461 gctgctcaac aacgagatgg atgacttcgc gacgaagcca gggcagccga acgtctacgg
     2521 gctcgtgcag ggcaaggcca atcaaatcga gcccgggaag cgcatgctgt cgtcgatgac
     2581 gccgacgatc gtcgaggatg aaaagggcga gctgtacatg gttgtcggcg ggcagggcgg
     2641 accacgcatc atcaccgagg tgtggcaggc gatctcgaac gtgatcgatt tcgggatgac
     2701 gcccgatgca gcgatcatgg cgccgcgctt tcaccatcag catcttcccg atgaagtcgt
     2761 tgttgaagcg gaagcgatta cgcgtgatgt cgacgaagcg ctgcgtgggc tcggttacac
     2821 gatcgtgtgg aaccaacctg aacgcatctt cggcgctgcg aacatgatcg tgcgaagcgc
     2881 aactggttgg gcgggcagcg cggatcgacg cggcggcggc gctgcgctgg gtgattagcc
     2941 gcgcgaagcg tggcagactg atctcccatg tctggtcagg gtgaacccag cggcagtgtc
     3001 gtctacccga agctgccggt tccctccaac cgaaacgcga gcgaggcgaa ggtgaagggc
     3061 ttcaaccaag acgacggata cagcaaaccg aagatcgcgg cagcggtcgc gctggtcgcg
     3121 gcgatcggtg gcgccgtcgg ctacttgctc gcgcccgaca agggcaaaga gctggcggcg
     3181 gcaaagaagg aagcggctgc acagcagacg gcggcgaaga ccgagaaaga tcgcgccgac
     3241 ggcattgcga agcaggtcga cgtcctcacc aaggacaaga ccgagctcga gaagcagctc
     3301 tccgacatga cggcgaaagc cggcgagatg gagaagaagg cgcaggccgc gaacgaggct
     3361 gcgcagaaga agctgaagaa cgcgatcggc gacgaggacg gctcggtgtc gaccgagggc
     3421 gaagagattc acctcaagct cgtcgacaag gtgctgttcg cggtcggcga cgatcagctc
     3481 accgacaagg gcaagacggt cctcgacaag gtcgcgaagg cgctcaagga cattccggac
     3541 aagcagatct gggtccaggg ccacaccgac gactcgccga tcatcgtgcc gccgccgccg
     3601 aagaaagatc cgaagaagaa gggcaaggac aaggagcccg agctggtgcc gttccgcttc
     3661 aagtcgaact gggagctgtc ggccgcgcgc gcgctccagg tcgtgcacta cttgcaagat
     3721 cacgcgaagg tcgatccgac gaagctcgcc gcgctcgcgt ttggcgagta ccgcccggtc
     3781 gcaaagaaca acaaggccgc gaaccgccgc atcgagatcg tgctctaccc gcatcgcgcg
     3841 gtgatcgagc gcgcgaagaa gtaattcccc gtcggggatg ccaagcgctc agcgctggcg
     3901 ctcgacgaag ctgacgaggt cgctgtcggc ggcgtgctcg agcgcatcga gctcgcccgc
     3961 accgctcgcg ccatcgcgcg cgaacaggag caccggcatc ggctcgagct ctctgcccga
     4021 gcgcagctcg cgcacgactt cgtgcacgcc ttcgctcgtc agctcgagca agatcgcgcg
     4081 cggccgtagt gcgcgtgcaa gtcgcgacag cggcgcgagt gcgcgcgtca tcgacacgac
     4141 cgtgtagccg cgttcgctca gcacgagatc gagctgctgc aggcgcgacc gatcgcgctc
     4201 gacgatcagc acgacgccgc gatcgctcgg ttcgggcagc ggctcggtcg ggttcgtgac
     4261 gtacggaccg tgcgtgagtc gatcggcatc gcgcgagcga tacacggcaa gcgcgacctt
     4321 gttgagcaga tcctcgacgt cgaacggctt ctcgacgacc tcgctcgcgc cgatcgagga
     4381 caggttgacc tcgaggccgt gcacggccgt catgatcagc accggcacgt tcacgtaggt
     4441 ggcgtcgttg cggagggcct gcaggaactg ccgcccgttc atgccgggca tcatgaggtc
     4501 gagcaggatg acgtcggggc gcgccgcgtg gacgcgatcg agagcatcct cgccgtcgac
     4561 agcaacatcg acgacgtagc cctcctccgc gaggacctgg cccagcgtct cccgcaccat
     4621 cggctcgtca tcaacgacga gaatccggcc gcctttcgct gcagctcccg tcattccggt
     4681 ttgacggagc gtaccacccg ctgtgcctcc ggggtacgat ggcaacgatg tcgacggcac
     4741 catcggtggc tcgtgcctac gacgggctac gcaccaaaat cctgcagcgc attctggaca
     4801 tgcgcgccac gatcgatccg tcgcttggcc gcgtcgacga ggccactgca cgtacgcaaa
     4861 ttggcgcagt gctcgatcac atcggcaact tcatcgcgac tggtgacctc ggccttcacc
     4921 gcgcgttctt gcacacgttc cttgccatgc gcgcagcgga ggcacaaggc ccggcgcagg
     4981 tgctcgcgat gctcgtcgcg atcggcgaca ccgctgcgca agtctcgcag gaagaaagtc
     5041 agggcgccga gggttccgag ctcacgctcc tgctcacgcg agtgaccgca gcgaccgcac
     5101 gtgcggtcaa cgatctgatc gccgacgagc tcgagcgccg gctcgcgcag tgggccgagc
     5161 tatcgaccaa agagcgagag gcactgaagc cgtcgtgagc accgcggtcg ctcccacgct
     5221 caaggtcgcc ggcgatacgc acgtcggcaa ggttcgcgcg accaacgaag actcgatgat
     5281 ctgcgatccg gtgcgcggcc tctacgtcgt cctcgacggc atgggcggtg cgaacgcagg
     5341 cgacatcgcg tcgcaaaccg cacgcgacgc gattcgcgac ttcgtcaccc agcgccggct
     5401 cacgatggag ccgcgcgcac tgctcgaagc agcgatccag tacggctcgg ccgcggtgtt
     5461 tgccgccgcg caagctgcac gcgagcgaca cggcatgggc acgaccgtcg tcgcgtgcct
     5521 cgtcatcgac accaagcgcg tgatcatcgc gcacgtcggc gattcgcgcg cctacctcct
     5581 ccgccacggc cgcctgcaat cgctgacgcg cgaccacacg atcgtcgagg agctcgtcga
     5641 tcgcggcgtg ctcgcggccg aggaggccga gcgtcatccg tacaagaacg tgctgtcgcg
     5701 caacctgggc gcgaaggccg aaacccgcgt cgacgtgctc gagctcgaca tgaagccggg
     5761 cgaccggctg ctgctctgct ccgacgggct ctacggctac gcctcgaccg aaggcatgca
     5821 gtacctgctc ggctccggcg atccgcccga agtcgtcgcg cgcgatctga tcgatctcgc
     5881 gctgcgcggc ggtggtggcg acaacgtctc ggtgatcgtg ctcgaagcac cgcaactcgc
     5941 ccccacctcg acgcaggtcg tccgcacgag tggcgcgacc gcgtggtggc agatgcgcca
     6001 gcgcttcctg caagtcgcga aggagcgcgg catcacgaag aacccgatct gccgcggcct
     6061 ccagcccgac gaggcactcg atctcgtcgc gctctcgctg tgccaggcga tcttccacga
     6121 cctcgagaag tccaccggcg tcaacgtgtg gacgttcgcg cagaacatcg cgggcggctg
     6181 gttcgagcgc ggcggcgagt ggtccgcgct gcgcagcctg atggacatcc tcgcgatgag
     6241 cgcgcgtacc gtcgtcgacg aaattcgcgc ggccgacgcc aagctcggct tcttgctcga
     6301 cgtcgcggtc tctcgcgctc tcgtcgttgc cgagctcgca ctcggtggcc tgctcgccga
     6361 gcgcctgcgc caggtcgaca gcgacctgat cgcggtccac aacgcgatcc aggacacgat
     6421 cgatgccgag gtcgacgacg tcatgtcgga cgtgcgcttc accgagcaag cgacgatccc
     6481 attcctgcgt cccgatcgtc cggtcaccca tcccggcatc tccgccgagc tggtctccgg
     6541 gatcgagaag acgctcacga tcgcccgcgc tcgcacgtcg ccgcgcgccg agctcgtcaa
     6601 gcagatcctc tcctcactcg aaacgatcgc gactgacacc gcgggcaact tctcggcggc
     6661 agtcctcgcc gcgcgcgagc tctacggcgt tcgcaccgtc gacgatgcgg gcatcatgtc
     6721 gctgttcgac gcgctcgatt ccgcccgcat catcaccgcc tcgtcggtcc accagacctc
     6781 gttccccgac gcgcttcgcg ccaaggttct cggcgcgatc tcgtccgcac accaacgcct
     6841 cgtcggcgcg atgaccggcc tcgtgctcga agccgtcgcg cctgtcaccg agaagctcaa
     6901 ggctgcgcaa gccgtcaccg ccgagctgcg cgacaaggtc ggccgcgccg agcgtcaccg
     6961 cgccgagctc gcggaaaaat tcgcgaccac catcgatcaa tccctgccgt gggaaaaacg
     7021 cggcgtgacg gagtggtgac atgagtgacg tcgatctcgt cgaagcggtc agcgtcctcc
     7081 gggacgaagt actcgatact gtcgagcacg gcgatcgcga tccgcccggc gccgaagtct
     7141 tcgacgcgct cattcgcgca ctcacgatcg gcggcgaatc cgtccccggc ctcgatctca
     7201 cgctccacga ttcggtcacg cgccggctcg cctggggcga cagcgaagag gtcgtgctcg
     7261 ccgacgccga gatggtgttc gatcgcctca tggtcgcggt cgagcgcgcg ctccgtgatc
     7321 ccgccgatcg catggtcgtc atcgaggccg ccacgcaggt cgcggtcacc gtcgcgcgtg
     7381 tcgtatcgct tgccgccgtc ggccgcgcat cacgcgatcg cgccgcacgc ctccgcgagg
     7441 agatggcgca gcgtcagctc aacgaagtgc tcgacaagca gcgcgcgagc atcgtgaatc
     7501 tcgagcgcga gacccgcgat cgctaagacg ttgccgtcgt cggcagtggg acatcgagct
     7561 cggcgagctc ggcttccgag atcgtcaggt tcgcgacttt gtgcgcgatg agtagtgcgt
     7621 gctttagctc gcccgtcagc gtgatcgtga acgtgccgct cgaaggatcc ttcgtcagct
     7681 tgtaggtcac gaactcgccg atcgagtacg actccagctc tggcaagccc atgccgtcga
     7741 ttatggtcgc gcactcgcgt gatggaaatg gggcgcgcgc gcagagagtg cccgagagtg
     7801 catcgacgca gaacgtcgta tcgcgcctca cgcgttatgc ggagggcgtg atgttcgcgg
     7861 tgatcgcgtc gagcagcgct tcgaggcctg aatcgcgatc gacgtagcga tgaacgagat
     7921 cggtgttggt tcgaccggtg ccgcgcacga tgcgacgagc tgcgggctgc gccttacgaa
     7981 cgtcatccat gatcggctcg atgcgcggat cggccgcgtc gagctcggcg atcaccgcat
     8041 cgagcttgat gtgctcgagt gcttcgtgca ccgcgtagtc gtcactcgcg aacagcacgt
     8101 cccagccact cgacacgtag agtgccatcg cgccgcgctg gcgatgatcg tcaccgacca
     8161 cgagcacgcg catacggcgg cctggttttt cgatcgttcc cgactgccgc gcggccgcgg
     8221 caacgagtgc gcgccactcg ggtggcggct cgatgagcag aacgccgagc gcgggggcag
     8281 tgcccgcgac ctcgcgcacg acatcgccga tcacttccat cgcggtgccc gaccgcggat
     8341 cggtcatggt gagcgagacg cgtgtgccga ccggcagcca ctcggacact tcggaggtgt
     8401 ccaggcaaat accgacctcg gacacgtcac gggtatggca gaccgccatc ttgccgccga
     8461 gaagcgggca caccgtgacg tttagcgaga tccggtaacg aggcgcgcga cgcgacaccg
     8521 agggtcattg taacagctgc cccactcgcg gcgtgggaat cgcggcatga taggtctggc
     8581 caggctggct tgcattgacc cgtgccagcc cgccgaggga aggtctggga cctatgagag
     8641 ttgctctcgc gatcgcagca ttgatcgcag cgccgctgac cgcagcggcc gacgagcccg
     8701 cgccgagcgc accgtctgcg ccaccaccga ccgcgggcct tcctggcatg ccaacaccaa
     8761 cgccgacgcc ggcaccgacg ggtggggcac cgggcgacac gccatcgacg accgcaccgg
     8821 caccggcacc ggcaccggcg ccggcaggcg atgcgcccga tcccgcttac gacacgcgga
     8881 actttcccgc cccgaaaggt gcggacgtcg tcatcgtttc gtacggcgag cgcagccgga
     8941 agaatgtgct gatgctcggc gggatcgctg cggccggcgt ggttttcggc gcggttggcc
     9001 tctacttcca tctcgactcg cggtctgcat ccaacgaagt cagctcgaac aagcccaccg
     9061 gcgagccgtg gacgcccgag cgcgccgata cgtacgatcg cgtgcacagc tcgtcggtcg
     9121 ccgcgggcgt gttctacggg atcggcggag ccttgctgct cacgagcgcg gtcatgtaca
     9181 tcgtcaccga gccgaaggaa gagaagacgg tgatccatcc gcactacgat ccgaagccga
     9241 ccgcactcgt cgcaccgacg cgtggtggcg ccatgatcgg cggagcgtgg aggttttgat
     9301 gcgcgcgctc ctgttgtgca ttccgctcgc cgcatgcacg cccgatgtcg tgtcgggctc
     9361 gtatctgtgc ggccctgatg agctgtgtcc cgacggtcag aagtgcaacg gcaccgaaga
     9421 caagaccacc ggcctcgccg ccgattcgtg cgtgatcccg tcactcgcgc ggccgtttgc
     9481 gtgtacgccg aaggtcgacg tcgagcccga cgacagcatg gctcaggcgc acg
//
LOCUS       DASUFX010000165        13334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_860816,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000165 DASUFX010000000
VERSION     DASUFX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860816"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2187)
                     /gene="mgtA"
                     /locus_tag="VFV99_06580"
     CDS             complement(<1..2187)
                     /gene="mgtA"
                     /locus_tag="VFV99_06580"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX5059006.1"
                     /translation="MRTYWAKSPAEVARSLGVTLDGLSSAFAAHSLELYGPNEIHARP
                     AATRLRVLWRQLRSPLVLLLVFAAIASTVSGEWVDASIVGAILGASVGVGYFREYRAE
                     TAIAKLLERVQIGTSVVRDGVQRAVPVREVVPGDVIVLAAGSIVPADAVLIEATELHV
                     DDAVLTGESFPVLKCVGPTAESAPLRDRSGCVQFGTNVRSGSARALAVATGKDTAFGA
                     IAQRLASRTPETEFERGLRRFGMLLLVAMLVMIVVVFAVNVLLGRPVIDTLLFAIALA
                     VGLSPELLPAIVGINLSRAATTLATTGVLVRRLDAIENLGSMTVLCTDKTGTLTEGVV
                     RVAGACDARGVASDTVIELAALNAALQAGFANPMDAAILDARAVDRSAIEKLAEIPYD
                     FTRKRVSVVVRNEHAVRLVMKGAFGHVVAVCTRLSDGTPIDATVRAELDARDRAWGAG
                     GIRVLAVATRTLESRERYSVDDEQDLTLAGFVTLYDRPKEDAGAALAKLRELGVRVKM
                     ITGDSRYVSRHVAREVGLGDAKLLTCEDLHDLTDIALVHAAMDADLFAEVDPNQKERI
                     LRALRKGDAVVGFFGDGVNDAPAMHAADVSISVESAVDVAKATADLVLTRKGLDVITR
                     GIEEGRHTFANTLKYILTTTSANLGNMISMAAASLVLPFLPLTAGQVLLNNFLSDIPA
                     VGIAGDNVDPELVAGPRRWDTHFIGRFMLEFGLLSSAFDALTFVVLI"
     gene            2377..3150
                     /locus_tag="VFV99_06585"
     CDS             2377..3150
                     /locus_tag="VFV99_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5059007.1"
                     /translation="MSFESHAQRPPFYDEGYGFDLFGLHPPAVERAVAAGAPFYDHYF
                     RVDSRGVEQIPETGPAILVANHGGVLPLDAAMLCLDVLRQTRRLPRAIADHFVPRLPI
                     VGTLFARIGVVSGARRNVERLLERGELVAIWPEGVTGPAKPFRSRYQIQQWRVGFAEL
                     AIRYRAPVIPAAVVGAEESWPMAKKLPGLRWFGVPYMPIPAFPVPLPTKLYIRYGQPI
                     RFDRVAADDPAVVAEEAHRVRVAIEQLIDETREERRRDQ"
     gene            3147..4133
                     /locus_tag="VFV99_06590"
     CDS             3147..4133
                     /locus_tag="VFV99_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5059008.1"
                     /translation="MNVLVTGATTPLGAAIVDELQRTSAAFVLAVGHEPCATYLHPGE
                     RVEYHSLDLTRPRSVHDLLWGPTRDHAIDTIIHLAQHRRVGDSGSRIHAQNVGATREM
                     LLGAMEHPTIRRFIYRSFSDVYALRHTTTELIDEEAPLDFEPQAPQWVRDRVEADTLV
                     SAHFGSRLQVAVLRCVEILASDVGSQLWDYLQSRVCLRPLGFDPMINVLSLADAAIAF
                     TAAAGSTETGVFNIVGADTLPLSRAIEESVRIEIPVPGPLMAPLYNLRRSLAGFEFNY
                     QMNLRRFHFGGVLDGTRAAERLGYVPQHHVEWPRPWWRMLIDRLTALRLAPQ"
     gene            complement(4173..4661)
                     /locus_tag="VFV99_06595"
     CDS             complement(4173..4661)
                     /locus_tag="VFV99_06595"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5059009.1"
                     /translation="MYRTCIVVADAARARLFTFERTAEAEGLAEELIERRDLVNPARR
                     LRSSKLFSDSRPGVSRMGKLQYAFDDHRDAHIDAFDAEFSRMIVGEVAAMMRATHAKR
                     LILCASPHMLGALRDASSGVGGDITIDEMPRNLVKLSPSRLRDQLAEYRLLPERPARQ
                     QV"
     gene            4820..5689
                     /locus_tag="VFV99_06600"
     CDS             4820..5689
                     /locus_tag="VFV99_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5059010.1"
                     /translation="MRFSKILCPVDFSPGSQQALRVATRLANEANAELVVVHAWYVPA
                     IAYSEFTFPADAIQGMADDANRALETTIAEAKRAGAKQISGKLLTGEPWSRIVGELDE
                     HGYDLCVIGTHGRTGLARVLLGSVAEKVVRHSPCSVLAVRPEGEVKPFKHPLVPTDFS
                     ASAEHALEMAVELAPEAITLLHVIELPVATGRTAVPNLERSLDESAMIALAEAANRLG
                     GKARGSITTRTRIGYPGAQTLAELDDDRSIDLVVMGTHGRTGVARALLGSVAEKVVRH
                     ARCPVLVARKVRS"
     gene            complement(5740..6837)
                     /locus_tag="VFV99_06605"
     CDS             complement(5740..6837)
                     /locus_tag="VFV99_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5059011.1"
                     /translation="MPQVDDDVYRVLGVSPDADAETVKAAYRAIAHENHPDRRPGDAA
                     AAAKFQAASHAYAQWMQANGERPAQAAPGQAAGFSNIEDIFSQFQDLFGDFFGAARTQ
                     PRDRGADLRLDLELTENEAIVGCKRDIEVNRGRVCTDCAGVGGFGEPTTCKDCAGTGK
                     QNMQQGFFMVQTTCAACRGHGKRWAKPCACERGLVRRAEKLAVVVPAGIQHGQILRLA
                     GKGDELAGKPTGYLYVAISIEGYETPGTAPPNGTPRDRGNDVVVDAAVRARHLWFGGT
                     LEVPTPDGSATVKVPRNIEDGHEIRLARRGKALAARSGEGGDPYRDVARGDLVVVLRV
                     TPAVQKRREAVGFAALASIVIAILVAVSHVV"
     gene            6933..7355
                     /locus_tag="VFV99_06610"
     CDS             6933..7355
                     /locus_tag="VFV99_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059012.1"
                     /translation="MHRFYVPGSRVIGGRFVTLTAPPAPYVDGGTLAAPTSIRGVPIP
                     AGAELFVDCDFDHIQVRFHAPVRVRGVPLVAGDEIQFLGRTLGFPGLRLPWWTLPLVL
                     VALPVLMIWRRRTTRDIWVMRDRKYVFAIKPDGSTTAI"
     gene            complement(7366..9036)
                     /locus_tag="VFV99_06615"
     CDS             complement(7366..9036)
                     /locus_tag="VFV99_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5059013.1"
                     /translation="MRWILLLVVACSSSSSPPAPETKSAPPKVSAEKLERGKPIEKAI
                     RKGESHRYRVEAGAAMVVTGVVMQNGIDVALHTYDPRGKHLAELDSPNGNDGPEPFVI
                     ETKRAGAYDIEVRPFVEPLPDGTPAPAEGRYEIRVDDIITADAYAEQLARQHIDSPRI
                     LDMWRAARARDRAALDKLWADLKGKAPIIEPYPEDKDSRLVTFVMRSKATYVGMLGAP
                     MARDTERAMLRIGDSDLWYLTGRIPAEAHFDYAFIETDGPPPLHEPYRGGAVRGVDQR
                     FAKRMQDPNNPLTHEGSSRVEVPGPPDEPFLVDNAATPKGTVTRIEIASSKLGENRVV
                     GVYVPAGFDAKQRYPLVIAFDGESYGLDPGSQIPLPRILDNLIAAKKIPPVVAALVAT
                     QAQRRRDLAESAPFAAFVVEELVPKMRADYHAGLTAADTLVTGSSLGGTESLYVGLHH
                     SNVVGNVLLNSAALWQRPHQFDGDPPDYIEGGAMIRELVKAAKLPLRFYVDTGLFEAH
                     LRDSNRRLRDVLEAKGYPLTYVEFPGGHDYSIWRHTIADGLIALLAKH"
     gene            9290..9637
                     /locus_tag="VFV99_06620"
     CDS             9290..9637
                     /locus_tag="VFV99_06620"
                     /inference="COORDINATES: protein motif:HMM:NF022703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEX5059014.1"
                     /translation="MKRRFLLGVVFTAVTVGPAIAEPTWKLAASDSSYSYVCGGDDWV
                     AINGKGNSITIRGECAIIEINGSGNKVSVESVGSIRISGNNNDLRYDRAPSGKTRPEI
                     KNKGAANTIRRNQ"
     gene            9760..10305
                     /locus_tag="VFV99_06625"
                     /pseudo
     CDS             9760..10305
                     /locus_tag="VFV99_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            10478..12061
                     /locus_tag="VFV99_06630"
     CDS             10478..12061
                     /locus_tag="VFV99_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5059015.1"
                     /translation="MRAAIALIAAAMVASCYATDEETAPALESQLRWISIDVDALDTA
                     RAIAPIDSVEVAGNIALVRFDERDLPSLSRAMHEQHNRCAGFIVHDTYEEGQLALAAA
                     NRDRTADFAPAYTIDNAAAVNRLLPALSESSLLMMIQQLSENYTSRYYNTSGGLAVPM
                     WLRDQWAGYAADRPDVTVELFTHQGFEQRSVIATITGSTKPNEVIVIGAHIDSIASGS
                     STAPGADDDASGIATISEVLHALMAKKFRPERTVKLMAYAAEEIGLKGSKSIVADYVA
                     KGINVVGVMQFDMTNYKGSPKDIYLMQDFTNAAQNTFVGNLIDTYVGATWGTSQCGYG
                     CSDHASWHNAGFAASMPAEATFEDTNPNLHTPNDTLEASNNTATHALKFARLATAYIA
                     ELAKGELMNAAPVVSITAPGAGETLTGSTPIELDATAMDDEDGELSASIEWTSSLDGA
                     LGSGASLKAKLSAGVHTITATAKDSAGATGEATMSLTITVGDGDDDGGTSSGCDAGGG
                     SGLAAACASLLAALRRRRR"
     gene            complement(12143..12556)
                     /locus_tag="VFV99_06635"
     CDS             complement(12143..12556)
                     /locus_tag="VFV99_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5059016.1"
                     /translation="MATLARDLMQANLVAVSPSSSLADVVHTLVVAQVSGVPVVDATG
                     AVVAVLSATDVLQAFEQALDEDNDEDEPAELADRLSAVTAGELATPDIVFVSPDAPVA
                     DVAQLMRAESIHRVLVGTRERVEGIITAFDLLAAV"
     gene            complement(12897..>13334)
                     /locus_tag="VFV99_06640"
     CDS             complement(12897..>13334)
                     /locus_tag="VFV99_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059017.1"
                     /translation="ATPTCEFVGVEWRPHLVTVANKLARDLAIPNVRFICGDALDVDW
                     GGYDAFYLYNPFAESLWGETFKLDSTIDHEKHDFASYVAGVRERLACAPVGTRIVTYH
                     GYGAPPPLGYELSVCEPGSSQLLELWVKTTPITGEWNTGALEV"
BASE COUNT         2351 a         4488 c         4304 g         2191 t
ORIGIN      
        1 gatcagcacg acgaacgtta gggcgtcgaa cgcagagctg agcaatccga actcgagcat
       61 gaaccggccg atgaagtgcg tgtcccagcg gcgcggaccg gccacgagct ccggatcgac
      121 gttgtcgccg gcgattccga ctgccgggat atcggagagg aagttgttga gcagcacctg
      181 accggctgtg agcggcagaa acggcagcac cagcgaggcg gctgccatgc tgatcatgtt
      241 gccgagattc gcgctcgtcg tcgtgaggat gtactttagt gtgttcgcga acgtatgtcg
      301 gccttcctcg atgccgcgcg tgatcacatc gaggcccttg cgcgtcagca cgagatcagc
      361 ggtcgccttc gcgacgtcga cggccgactc gacggagatc gacacatcgg cggcgtgcat
      421 cgcaggcgcg tcgttgacgc cgtccccgaa gaaaccgacg actgcatcgc ccttgcgcag
      481 cgcccgcaat atgcgttctt tctggttcgg atcgacttcg gcgaacagat ccgcatccat
      541 cgcggcgtgg acgagcgcga tgtcggtgag gtcgtgaagg tcctcacacg tcaggagctt
      601 tgcgtcgccg aggccgactt cacgtgcgac atggcgagag acgtaccggc tgtcgccggt
      661 gatcatcttc acccgcacac cgagctcgcg cagcttggcg agcgccgcgc cggcgtcttc
      721 cttcgggcgg tcgtagagcg tcacaaaccc ggcgagcgtc aggtcctgtt catcgtccac
      781 gctgtagcgc tcgcgcgact cgagcgtgcg cgtcgcgacg gcgagtacac ggatccctcc
      841 ggcgccccac gcgcgatcgc gtgcgtcgag ctcggcgcgc acggtggcat cgatcggcgt
      901 gccgtcggac aatcgcgtgc acaccgcgac cacgtgcccg aaggcgccct tcatgacgag
      961 ccgaacggcg tgctcgtttc gcacgacaac gctgacgcgc ttgcgcgtga agtcgtacgg
     1021 gatctcggcg agcttctcga tcgcgctgcg atccacggcg cgcgcatcga ggatggccgc
     1081 gtccatcggg ttcgcaaagc cggcctgcag tgctgcattc aacgcggcga gctcgatcac
     1141 ggtgtcgctc gccacgccgc gcgcatcgca agcaccggcg acgcgcacga caccctcggt
     1201 gagggtgccg gtcttgtcgg tgcacagcac cgtcatgctg ccgaggttct cgatcgcatc
     1261 gagccgacgt accagaacgc ccgtcgtcgc gagggtcgtc gctgctcgcg acaggttgat
     1321 gccgacaatc gcaggcagca gctcggggct cagtccaacc gcaagcgcga tcgcgaacaa
     1381 cagcgtgtcg atcaccggtc gtccgagcag gacattgacc gcgaacacga ccacgatcat
     1441 gacaagcatc gcgacgagga ggagcatccc gaatcgccgc aacccgcgtt cgaactcggt
     1501 ctccggtgtc cgcgacgcga gccgctgcgc gatcgcaccg aacgcggtgt ccttgccggt
     1561 cgcgacggca agcgcacgcg ccgaaccact gcgcacgttc gtcccgaact gcacgcagcc
     1621 cgaccggtcg cgcagtgggg cgctctccgc cgttggcccc acgcacttga ggaccgggaa
     1681 gctctcgccg gtcagcacag cgtcgtcgac atggagctcg gttgcctcga tcagcaccgc
     1741 atccgcagga acgatgctac cggcggcgag cacgatgacg tcgccgggca cgacctcgcg
     1801 aaccggcacc gcgcgttgga cgccgtcgcg cacgacgctc gtcccgatct gcacgcgctc
     1861 cagtagcttc gcgatcgccg tctccgcgcg gtactcgcgg aaatagccga cgccgacgct
     1921 cgcgccaagg atcgcgccga cgatcgacgc atcgacccat tcgcccgata ctgtcgacgc
     1981 aatcgcggcg aacacgagca gcagcaccag tgggctgcgc agctggcgcc acagcacacg
     2041 cagccgggtc gccgcgggcc gggcatggat ctcgttcggt ccgtagagct cgaggctgtg
     2101 cgctgcgaac gcggacgaca gaccatcgag cgtcacgccc agtgagcgcg cgacttcggc
     2161 cggcgacttc gcccagtacg tgcgcatacc tgctgtgtca gcctgcctgc tgcataagag
     2221 cactgcaaga caacaccccc ggtcgtctgc gtagcgggcc gtacacatgc ccgctaggca
     2281 tccgacgcag ccgctgcatg ccaccgctcc gtccgcgcgg cctttgcgcg ttcacgcggc
     2341 acgtagcgag cacgtggctt gctaccggga tagttgatga gcttcgaatc gcacgcgcaa
     2401 cgtccgccgt tctacgacga gggatacggc ttcgacttgt tcgggctcca tccgccggcg
     2461 gtcgagcgag cagtcgcggc gggcgcgccg ttctacgatc actactttcg cgtcgactcg
     2521 cgtggcgtcg aacagattcc tgaaaccggc cccgcaatcc tggttgcgaa tcacggcggc
     2581 gtgctgccgc tcgatgccgc gatgctctgc ctcgacgtac tgcggcaaac ccggcggctg
     2641 ccgcgcgcga tcgcggatca cttcgtcccg cggttgccga ttgtcgggac gctgttcgcg
     2701 cgcatcggag tcgtgagcgg cgcgcgcaga aacgtcgagc ggttgctcga gcgcggcgag
     2761 ctcgtcgcga tctggcccga gggcgtgacc ggccccgcca agccgttccg ctcgcgctac
     2821 cagatccagc agtggcgcgt gggattcgcc gagctcgcga tccgctatcg cgcccccgtg
     2881 atccccgctg ccgtcgtggg tgccgaggag agctggccga tggccaagaa gctccccggg
     2941 ctgcgctggt tcggcgtgcc gtacatgccg atcccggcgt tcccggtgcc gcttccgacc
     3001 aagctctaca tccgctacgg ccaaccgatc aggttcgatc gagtcgcggc ggacgatcca
     3061 gcggtcgttg ccgaggaggc ccaccgcgtg cgcgttgcga tcgaacagct gatcgatgaa
     3121 acacgggaag agcgtcggag ggaccaatga acgtcctggt aaccggcgcc acgacgccgc
     3181 tcggtgcagc gatcgtcgac gagctacagc gtacgagcgc ggcgttcgtg ctcgcggttg
     3241 gtcacgagcc gtgcgcgacc tacctgcatc cgggcgaacg ggtcgagtac cactcgctcg
     3301 atctgacgcg gccgcgcagc gtgcacgacc tgctttgggg gccgacgcgc gaccacgcga
     3361 tcgacacgat catccacctc gcgcagcacc gccgggtggg cgacagcggc tcgcgcatcc
     3421 atgcgcagaa cgtcggggca acgcgcgaaa tgctgctcgg tgcgatggag cacccgacga
     3481 tacgccgctt catctaccgc agcttctcgg acgtctacgc gcttcggcac acgacgacgg
     3541 agctgatcga cgaggaggcg ccgctggact tcgaaccgca ggctccacag tgggtgcgcg
     3601 accgcgtcga ggcagacacg ctcgtctcgg cgcacttcgg ttcgcggctt caggtggccg
     3661 tgctgcggtg cgtggagatc ctcgcatccg acgtcggcag ccagctgtgg gactacctgc
     3721 agtcgcgtgt ctgcctgcgc ccgctcggct tcgacccgat gatcaacgtg ctctcgcttg
     3781 cggacgctgc gatcgcgttt accgcggcgg caggctcgac cgagactggc gtgttcaaca
     3841 tcgtcggtgc cgacacgctg ccgctgtctc gcgcgatcga ggagagcgtg cggatcgaga
     3901 tcccggtccc tgggccgctc atggcacccc tgtacaacct gcgccgctca ctcgcggggt
     3961 tcgagttcaa ctaccagatg aacctgcggc ggtttcattt cggcggcgtg ctcgacggaa
     4021 cacgcgccgc cgagcggctc ggctatgtcc cgcagcacca cgtcgaatgg ccgcgcccgt
     4081 ggtggcgcat gctgatcgat cgccttaccg cgctgagact cgcgccgcag tgacgctccc
     4141 ggtcatggca cgcgcaccga gcgaagcccc cgtcacacct gctggcgcgc cggtcgttcc
     4201 ggcaacaagc ggtactcggc gagctgatcg cgcagccgcg atggagacag cttcacgagg
     4261 ttgcgcggca tctcgtcgat cgtgatgtcg ccgcccactc cgctgctggc atcgcgcaat
     4321 gcgccgagca tgtgcgggct cgcgcacagg atcaggcgct tcgcgtgcgt ggctcgcatc
     4381 atcgccgcga cctcgccgac gatcatgcgt gagaattcgg cgtcgaacgc atcgatgtgg
     4441 gcgtcgcgat gatcgtcgaa tgcgtattga agcttgccca tgcggctcac ccccggccga
     4501 ctgtcggaga acagcttcga ggatcggagc cgacgagccg ggttgacgag atcgcgtcgc
     4561 tcgatcagct cctccgccaa cccttcggcc tcggccgtgc gctcgaacgt gaacaagcgg
     4621 gcgcgcgcag cgtccgcgac aacgatgcat gtccggtaca tgcccctcgc cagtgcaaag
     4681 cgatgaccag gggcggcatg ggctgagtcg accagattgc gcaatcgctg cacccgacga
     4741 cagaagcggc gcggcttctt cgcgcgcttg gttgcgcgcg cgcgcaaatg ttggccggtt
     4801 cgttgcacga catcggggca tgcgcttctc gaagatcctg tgcccggtcg acttctcgcc
     4861 gggctcacaa caagcactgc gggtagcgac tcgcctggcg aacgaagcga atgccgagct
     4921 cgtcgtcgtg catgcctggt atgtcccggc gatcgcatac agcgaattca cgttccccgc
     4981 cgacgcaatc caagggatgg cggacgacgc gaaccgcgcg ctcgaaacaa ccatcgccga
     5041 agcgaagcgt gctggcgcga aacagatcag cggcaaactg ctcacgggcg aaccgtggtc
     5101 caggatcgtc ggcgagctcg acgaacacgg atatgatcta tgcgtcatcg gcacgcacgg
     5161 tcgaaccggg cttgcacgtg tcttgctcgg ctccgttgcc gagaaggtcg tccgtcattc
     5221 tccgtgctcg gtcctcgcgg ttcggcccga gggcgaggtc aaaccgttca aacatcccct
     5281 ggtaccaacg gacttctccg ccagtgccga gcacgcgctc gagatggcag tcgagctcgc
     5341 ccctgaagcg atcacgctgc tgcatgtgat cgagctaccg gtcgcgaccg ggagaacagc
     5401 cgtaccgaat ctcgagcgca gcctcgacga gagcgccatg atcgcgctcg cggaggcggc
     5461 aaatcggctc ggaggcaagg cacgcggatc gatcaccacg cgaacgcgca ttggctaccc
     5521 aggcgcgcag acgctcgccg agctcgatga cgatcgaagc atcgatctcg tcgtgatggg
     5581 aacccacggc cgtaccggcg tcgcgcgagc gcttctcggt tccgtcgcgg agaaggtcgt
     5641 acgccatgca cgctgcccgg tgctcgtcgc gcgaaaagta agatcgtaga ggcgcgctcg
     5701 ctcgcgaagc cggacccgcc gagcagatcg atgcgggctt cacacgacat gcgagaccgc
     5761 gacgaggatc gcgatcacga tgctggcgag cgcggcgaac ccaactgctt cgcgccgctt
     5821 ctgcaccgcc ggcgtcacgc gcagcacgac gaccagatcg ccgcgtgcga cgtcgcgata
     5881 cggatcgccg ccttcgcccg agcgggccgc gagagctttg ccacgcctgg caagccggat
     5941 ctcgtggcca tcttcgatat tgcgcggaac cttcacggtc gccgagccat cgggcgtcgg
     6001 cacctcgagt gttccgccga accagagatg tcgcgcgcgc accgcggcgt cgacgacaac
     6061 atcattgccg cgatcgcgcg gcgtgccgtt cggcggcgca gtgcccggcg tctcgtagcc
     6121 ttcgatcgag atcgcgacgt acaggtagcc ggtcggtttc ccggcgagct cgtcgccttt
     6181 gccggcgagg cgcaggatct gtccgtgctg aatgccggcc ggcacgacga cggcgagctt
     6241 ctcggcacga cgcacgagcc cgcgctcgca tgcgcacggc ttggcccacc gcttcccatg
     6301 gccgcggcac gccgcgcagg tggtctgcac catgaagaag ccctgctgca tgttctgctt
     6361 gccggtgccc gcgcagtcct tgcacgtcgt cggttcgccg aagccgccaa cgcccgcaca
     6421 atcggtgcac acgcgcccgc ggttcacttc gatgtcgcgc ttgcagccaa cgatcgcttc
     6481 gttctcggtg agctcgaggt cgaggcggag atccgcgccg cgatcgcgtg gctgggtgcg
     6541 cgccgcgccg aagaagtcgc cgaacagatc ctgaaactgc gagaatatgt cctcgatatt
     6601 cgagaagccg gcggcctgcc cgggcgccgc ctgcgcaggg cgctcgccat tcgcctgcat
     6661 ccactgcgcg tacgcgtggc tggcggcctg gaacttcgcc gccgcggccg cgtcgccagg
     6721 ccgacgatcg ggatggttct cgtgtgcgat cgcgcggtat gccgccttca cggtctcggc
     6781 gtctgcgtcg ggcgacaccc cgagcacgcg atacacgtcg tcgtcgacct gcggcacgtc
     6841 cggtctgtag cacggcttcg cggtgttacg ccgctggatt cgtcgacggc cgcgcgcaac
     6901 cgcgacgccc cgaccacgca tcccatgatc gagtgcatcg cttctacgtc cccggaagtc
     6961 gagtcatcgg tggtcggttc gtgacgttga ccgcgccgcc cgcgccgtac gtcgacggcg
     7021 gcacgctggc cgcaccgacg agcatccgag gcgtgccgat tccggccggt gccgagctgt
     7081 tcgtcgactg cgacttcgac cacatccagg tgcgctttca cgcgccggtc cgagtgcgcg
     7141 gcgtgccgct ggtcgcgggc gacgagatcc agttcctcgg tcggacgctc ggctttcccg
     7201 gcctgcggct gccgtggtgg acgctgccgc tagtactcgt ggcactgccg gttctgatga
     7261 tctggcgccg ccgcaccacg cgcgacatct gggtcatgcg cgatcgcaaa tacgtgtttg
     7321 cgatcaaacc cgatggctcg acgaccgcga tctgatccgc gcagctcagt gtttcgcgag
     7381 cagcgcgatc aacccgtcgg cgatcgtgtg tcgccagatc gagtagtcat ggccgcccgg
     7441 aaactcgacg tacgtgagcg ggtagccctt ggcttcgagc acgtcgcgca accggcggtt
     7501 gctgtcgcgc agatgcgcct cgaacaagcc cgtgtcgacg tagaaccgca gcggtagctt
     7561 cgccgccttc acgagctcgc ggatcatcgc gccgccctcg atgtaatccg gcgggtcgcc
     7621 atcgaactga tgcggccgct gccacagtgc agccgagttc aacaacacat tgccgacgac
     7681 attcgaatga tggaggccaa cgtagagcga ctcggtgccg ccaagactcg atccggtcac
     7741 gagcgtgtcc gccgccgtga gacccgcgtg gtagtcagcg cgcatcttcg gaacgagctc
     7801 ctcgacgacg aacgccgcga acggcgccga ctccgcgaga tcgcgtcggc gctgcgcctg
     7861 ggtcgcgaca agcgctgcca ccacaggcgg gatcttcttg gccgcgatca agttgtcgag
     7921 aatccgtggc agcggaatct gcgagccggg atcgaggcca tagctctcac catcgaacgc
     7981 gatcacgagc ggataccgtt gcttcgcgtc aaagcccgcc ggcacgtaca cgccgaccac
     8041 gcggttctcg ccgagcttgc tgctcgcgat ctcgatgcgc gtcaccgttc ccttcggcgt
     8101 cgccgcattg tcgacgagga atggctcgtc gggcggtccc ggtacctcga cgcgagatga
     8161 gccctcgtgg gtgagcgggt tattcgggtc ctgcatgcgc ttggcgaacc gttgatcgac
     8221 gccgcgtaca gcacctcccc ggtacggctc gtgaagcgga ggcggaccgt cggtctcgat
     8281 gaacgcgtag tcgaagtgcg cctccgccgg aatccggcca gtgagatacc agaggtccga
     8341 atcgccgatg cggagcatcg ctctctcggt gtcgcgagcc atcggtgcgc cgagcatccc
     8401 gacgtatgtc gctttcgagc gcatcacgaa cgtcaccagc cggctgtcct tgtcctcggg
     8461 atacggctcg atgatcggcg ctttgccttt gaggtccgcc cagagcttgt cgagcgcagc
     8521 tcgatcgcga gcacgcgcag cgcgccacat atcgaggatg cgcgggctat cgatgtgctg
     8581 tcttgcgagc tgctcggcgt acgcatcggc cgtgatgatg tcgtcgacac gaatctcgta
     8641 gcgaccctct gccggcgcgg gtgttccgtc gggcagcggc tccacaaacg gccgcacctc
     8701 gatgtcgtag gcaccagccc gcttcgtctc gatcacgaac ggctcgggac catcattgcc
     8761 attcgggcta tcgagctcgg caagatgctt accgcgcgga tcatatgtgt gaagcgcgac
     8821 gtcgattccg ttttgcatca cgacacctgt gacgaccatc gcggcgcccg cttcgacgcg
     8881 atagcggtgt gactcgccct tgcggatcgc cttctcgatt ggcttgccgc gctcgagctt
     8941 ctctgcgctg accttcggcg gtgccgattt cgtctccggc gccggcggag acgacgacga
     9001 cgagcacgct acgacgagaa gcaatatcca acgcatcggg ccgatgttct cactgcgaac
     9061 ggaccgaaca gcaaccggcc attcgggcgg gcatgccaca ccacaaaaaa caaacgagac
     9121 cgacgaacat atcgacgagc ccgtccccgg atggaacctg ggtgcccggg tctgacagaa
     9181 cgcgattctt tcgtgcccgt gcccacacac gcgcgaacgg ctcgcgtgga tcggcgcgca
     9241 cttgccgaaa acgcctgctg acatgcgcgc ggaggcatac gatcggccca tgaagcggcg
     9301 atttctcctc ggtgtcgtgt tcaccgctgt gacggtcggc cctgcgatcg ctgaacccac
     9361 gtggaaactc gccgccagcg acagctcgta ctcgtatgtg tgcggcggcg acgactgggt
     9421 cgcgatcaac ggcaaaggca actcgatcac gatcaggggc gagtgcgcca tcatcgagat
     9481 caacggctcg ggcaacaagg tctcggtcga gtcggtcggc tcgatccgga tcagcggcaa
     9541 caacaacgac cttcgctacg accgcgcgcc cagcggcaag accaggcccg agatcaagaa
     9601 caagggcgcg gccaacacga ttcgtcgaaa ccagtagatc gattgcgcgt aaccacgtgg
     9661 gcgtcgcggc gtagactggt cacgatgctt ctcctcgaca ccaagcagcc gacgacattc
     9721 gagacagcaa ctttcggact cggctgattc tggacgcctg acgcccggtt cgggtgtcat
     9781 ccggcgatct tgcgaacgcg cgtcggctac gccggcggca ccacgagtgc gccgacgtat
     9841 cacgacctcg ccgaccacac ggaagtgttc cagatcgact tcgatccggc gcgcctcagt
     9901 tatgccgacc tgctcgagca gatctgggcg aatcgccgag gcggccgttc gtacagcaag
     9961 cagtacatgg aggcagtgtt ctgcgcgaac ggacaacagg aacagctcgc gcgtgctcgc
    10021 ggtatcaccg cgccgatcat caccggcgcg cggttccatc tcgccgagga ctaccaccag
    10081 aagtactacc tgaggcacga cacgctgctg atgcgcgagc tcgctgacta ctcaccgcgg
    10141 cagctcgtcg actcgaccgt cgcagcacgt ctcaacggct acgtcgctgg caaaggctcg
    10201 gcgtcgcagc tacgcgagga gctcgcgagc ttcggcttgt cagcagccgc gaacacgcac
    10261 ctcgaaaagc tcgtcgggca acgtgcacgg atgacctgcg cctagcgcga agccccgtca
    10321 cgcctgacac ctgattgcgg aaggcctgcg caactcgagt agcagtggct gtcaggccgt
    10381 cgacaaaccg tcggtgcggt gctgccgcgc gcgtgcctca gtgcgagcag gtcgcacatc
    10441 gcccggctga cgagacaacg ttacggttct cgtcgagatg cgagcagcaa tcgcgctgat
    10501 cgcggccgcg atggtcgcca gttgctacgc caccgacgag gaaacggcgc cagcgctcga
    10561 gtcacagctg cgctggatct cgatcgacgt cgacgcgctc gacaccgcac gagcgatcgc
    10621 tccgatcgac tcggtggagg ttgccggcaa catcgcgctc gttcgattcg acgaacgcga
    10681 tctcccgagc ctgtcgcgcg ccatgcacga gcaacacaac cgctgcgccg gcttcatcgt
    10741 gcacgacacc tatgaggagg ggcagctcgc gctggccgcg gcgaaccgtg accgcaccgc
    10801 cgacttcgcg ccggcctaca cgatcgacaa tgcggctgcc gtcaaccggc tgttgcccgc
    10861 gctgagcgag tcgagcttgc tgatgatgat ccagcagctc tccgagaact acacgagccg
    10921 ctactacaac acgtcgggtg ggctcgccgt cccgatgtgg ctccgcgatc aatgggcggg
    10981 ctacgccgcc gaccgtcccg acgtgacggt cgagctgttc acgcatcaag gcttcgagca
    11041 acgctcggtc atcgcgacga tcaccggctc gacgaagccg aacgaggtga tcgtgatcgg
    11101 tgcacacatc gactcgatcg cgagcggcag cagcactgca ccgggagccg acgacgatgc
    11161 gtccggcatc gcgacgatct ccgaggtgct gcacgcgttg atggcaaaga agttccggcc
    11221 cgagcggacg gtcaagctca tggcctatgc ggccgaagaa atcggactga aaggctccaa
    11281 gtcgatcgtc gcggactacg tcgcgaaggg catcaacgtc gtcggcgtca tgcagttcga
    11341 catgacgaac tacaaaggct cgcccaaaga catctatctc atgcaggact tcacgaacgc
    11401 ggcgcagaac acgttcgtcg gcaacttgat cgacacgtac gtcggcgcga cgtggggcac
    11461 gtcgcaatgc ggctacggct gttccgacca tgcgtcgtgg cacaacgcgg gctttgcggc
    11521 gtcgatgccc gccgaagcga cgttcgaaga cacgaacccg aacctccaca cgccgaacga
    11581 cacgctcgag gccagcaaca acaccgcgac ccatgcgctc aagttcgcgc gcctcgcgac
    11641 cgcctatatt gccgagcttg ccaagggcga gctcatgaac gcagcgcctg tcgtttcgat
    11701 caccgcgccg ggtgccggcg aaaccctgac cggctcgacg ccgatcgagc tcgacgcgac
    11761 cgcgatggac gacgaggatg gcgagctgag cgcgagcatc gagtggacgt cgagcctcga
    11821 tggtgcgctc ggcagcggag catcgctgaa ggcgaagctc tcggccggcg tgcacacgat
    11881 caccgcgacg gcgaaggaca gcgcgggcgc gaccggcgaa gcgacgatgt cgctcacgat
    11941 cacggtcggc gacggagatg acgacggcgg aaccagcagc gggtgcgatg cgggtggtgg
    12001 ctcggggctc gcagcagcgt gcgcgagtct gctcgcggcg ctgagacgcc gccgtcgtta
    12061 gatcagctcg aagagctcgt cgaccgccat gtcgatgatc tcgcggatca cccggcatgg
    12121 atttgccggc cgccagcacc gattagacgg ccgcgagaag atcgaacgcc gtgatgatgc
    12181 cctcgacgcg ttcgcgcgtt ccgacgagca cgcgatgaat gctttcggca cgcatcaact
    12241 gggcaacatc cgcgaccggc gcgtcgggcg acacgaacac gatgtcgggc gtcgcgagct
    12301 cgccagcggt cacggcgctc agacggtccg cgagctcggc gggctcgtct tcgtcgttat
    12361 cttcgtcgag cgcttgctcg aacgcctgca ggacgtcggt ggcgctgagc acggcaacca
    12421 ccgctccggt cgcatcgacg acgggcaccc cgctcacctg cgcgaccacg agcgtgtgga
    12481 ccacatcggc aagcgacgag ctcggagata cagcgacgag attggcttgc atgagatcgc
    12541 gagcgagcgt tgccatgcga tccgctgcag caatccgtgg gccgtaccgt ggaagcgcta
    12601 cgaaccgcgg gcgctcagac gaacgccgtg atgcgcacgt cggtacttcg cgatgaacgc
    12661 atacaacgcc acgaggatcg gaagtccgag aagaatcaag agaagagccc caggatgctc
    12721 gtgcgcgttc cgactcacgg tgacgagtac gaggccagcg attagcggaa cgctggccgc
    12781 gagcgccgcc attgcgagat gcgacatgtt gttgacgtcg cggcgagtgg tgggacgatc
    12841 gcttgcactc attatgtgca caacgacatg caccggtcgc gccatgcggt ttgcgttcac
    12901 acctcgagcg ctccggtgtt ccattccccg gtgatgggtg tggtcttcac ccagagctcg
    12961 agcagctgcg agctgcccgg ctcgcatacg gacaactcgt agccaagcgg cggtggcgcg
    13021 ccgtatccgt gataggtaac gatgcgagtg ccgactgggg cgcacgcgag gcgctcgcgt
    13081 acaccggcga cgtacgacgc gaagtcgtgc ttttcgtggt cgatcgtact gtccaacttg
    13141 aacgtttcgc cccacagcga ttcggcgaac gggttgtaca agtagaacgc gtcgtacccg
    13201 ccccaatcga cgtcgagcgc gtcaccgcag atgaagcgca cgtttgggat tgccagatcg
    13261 cgtgcgagct tgttcgcgac cgttacgaga tgcggccgcc attcgacacc aacaaactcg
    13321 cacgtcggag ttgc
//
LOCUS       DASUFX010000166         8738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1415558, whole genome shotgun sequence.
ACCESSION   DASUFX010000166 DASUFX010000000
VERSION     DASUFX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415558"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFV99_06645"
     CDS             <1..992
                     /locus_tag="VFV99_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059018.1"
                     /translation="TAVGDNALEHECGRGGRIEHGRDVYRFTYLAKDGYGRWEIVLRE
                     QQIRDIAGGLLDEVDAVQLAEGTRTSRGDALIVWGEYDEDALRVRTKGDLGVALDALH
                     TVGIQEPCMVRLWSSSDDQAIAVFNGHDCAIYVVASEHGYGRSVGDPTRPDTFELTDF
                     RAGPVSIPWADVVPWRVARPALMMFAEKGQLGDNVILDGAIPTQFLFLSDFDRATELG
                     TRRQPVADPAQSSLPRKTPHGEWARRLVTSLLEIQLIEVDTQILEAVIARIAVLLIAH
                     DDALDSPDAAQKLAKEIERVRGVGALFATGGDLQIALRRTQDPPTQPTELPFT"
     gene            992..2335
                     /locus_tag="VFV99_06650"
     CDS             992..2335
                     /locus_tag="VFV99_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059019.1"
                     /translation="MRWLVGLVFVAFGCGDNLPPRGTFEVVGHSDLGARGMNSALAIA
                     GDIAYVGSRIDKKPVLIVDIKDPANPHVVGEIGEPAEGLPGMSSRELRATTNPPRLFI
                     MNLACSPDLHGCSGVPGEIENIKEYDISNPLAPQLLGSHGVTGSALRQRSPHEFFLWQ
                     DPSNLERVLLLISAPGVPSLEIVAAQDGDPKLLFDFDPYSEGGVTRGGENNILHSVSA
                     SDDGRMLFLSHQIAGLYIADASSVIDMVDPLQLTMLTTADAAVKFGAMGPHSAVAVPG
                     RKLLVTTEEVYPPPFGAGCPWGHMRLVDIANPAEPKLRGEYKLPENDPSCTAAGPMVA
                     FTAHNATVMNNIALITWYAGGLQAIEISDPDAPYQLGEFKPEPLPSVTVEDPGLSGSR
                     VEMWSYPIIKDGLIYVVDVRNGLYVLRYNGWRADEILARKFLEGNSNIGSFTPRI"
     gene            2377..2742
                     /locus_tag="VFV99_06655"
     CDS             2377..2742
                     /locus_tag="VFV99_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059020.1"
                     /translation="MTVARSALFAAAVATAAFGCHRRHQRNPEPALDLETSKPDRTVE
                     QTPPSQSEGFGTAAPNGGGALGGAVVSSAQGRTKIIQMRSRAKHHYNDTSQNHVEETA
                     PGDQPTQGIGDGDTYERNR"
     gene            complement(2743..3816)
                     /locus_tag="VFV99_06660"
     CDS             complement(2743..3816)
                     /locus_tag="VFV99_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HEX5059021.1"
                     /translation="MKRLAVAALLLAACGGGGDEECTIDGKMCSRLSSWQLFDDIASQ
                     TPAAGVIPYDLTTPLFSDYTTKQRFVRVPDGTPATWTADDPLDLPVGSVLVKTFSYLH
                     DRRDPANGRTLLETRVLVHGEKGWHGSSYTYGNETDDAKVAIAGAIIDAEWIHDDGSM
                     RTNQYNVPNQNQCKLCHEEHDEIVTPVGPKVRNLVPDRVQAMIDSGALVDAPPRDQWP
                     RAVNAFDATSGTLDERARAWLDINCGFCHNPRGAARTSGLYLDHAETDPAKFGLCKAP
                     VATGRGSGNLQYDIVPGNPDESIMIYRISSTEPEIRMPELGRNLVHEEGVALIREWIA
                     SMAGACSGSPASGDDRPSQRQTR"
     gene            complement(3813..4931)
                     /locus_tag="VFV99_06665"
     CDS             complement(3813..4931)
                     /locus_tag="VFV99_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HEX5059022.1"
                     /translation="MQTALIEIESGGTVAFGAGTFNVRTDLSLDVDNVTIKGAGMDAT
                     TLSFKSQTSGAQGLLVTSDAVTIHDIGIEDTRGDALKVLGSTDVTIDKVRVEWTGGPN
                     ATNGSYGLYPVQCKNVKITNSVVRGASDAGVYVGQSDTILLHDNLAEDNVAGIEIENS
                     MHADVFHNTATRNTGGILVFNLPGLEVPNGAITRVYENDVSGNNTENFAPAGNIVGLV
                     PTGTGIAVLAAHQVEIFDNRVSDHKSINVGVISYVPTGIVVTDPTYDQYPTAINIHDN
                     TLTGTSDMPTGMLGALLISALGEIRPNGPFIVPDVAWDGVLDPARTYGPEDRICVKNN
                     GDADFIDLKWPLADATVPTEDMAPHDCALAPLPAVTLP"
     gene            5135..6100
                     /gene="ligD"
                     /locus_tag="VFV99_06670"
     CDS             5135..6100
                     /gene="ligD"
                     /locus_tag="VFV99_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5059023.1"
                     /translation="MQWIPGVRLPKQLQLALLVDEAPDGDDWLHEQKFDGYRIVAELE
                     GRRLRLLSRRFNDWTAQFPTVVGAVAALKVKSVLLDGEVAVVMPDGRTSFQALQNAFG
                     RRDANLAYFVFDLLALDGEDLTKLPLEERKQRLAKLIGKRQGVLRYSDHVIGNGKAFF
                     ELACKKGLEGIVSKRRDAPYSPGRGKGWVKTKCLMRQELVIGGYTDPEGARTHIGALL
                     VGYYEGGALRYGGKVGTGFTMKDLVKIKQALSPLEVATSPFEPAPPRAWTGPNRHWVS
                     PTIVAEVAFSEWTNDGRLRHPSFQGLRADKPPNEIVREQATHVNR"
     gene            6115..6561
                     /locus_tag="VFV99_06675"
     CDS             6115..6561
                     /locus_tag="VFV99_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059024.1"
                     /translation="MRCALVFLVLLACSCKNEGSQRAPVGSAAGSAIAKPIIPDDVLV
                     GRWFAAMEKQLDADARALAVQKFASREDVEHALMGLTPAGFKPRPEFFQAFYAEAGEP
                     TTVAKRLADYASTHGDDMERRTDAMLKRLEPAMKLAADNAERQFAR"
     gene            6834..7049
                     /locus_tag="VFV99_06680"
     CDS             6834..7049
                     /locus_tag="VFV99_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059025.1"
                     /translation="MADDVKAAMQSEGAAVNGTMFLGTPLDLIEELERCTQLSAASVV
                     LVGRFAHDEVADALRDIYPGLEVRLAA"
     gene            complement(7046..8641)
                     /locus_tag="VFV99_06685"
     CDS             complement(7046..8641)
                     /locus_tag="VFV99_06685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059026.1"
                     /translation="MTTRGSISVMTNAADALRAEEIKRTRSLLGVGWIAGIVVAVAVL
                     IAPGDRRIAHALLALLGIGVLGSLWVYRDLRDATRYRTTTMTLLALVVVSCGVLGILY
                     TGTFSAAPVGLTLCIYIFCRTENLLSAIAIYAIAAIAHFVASMLVIAGVFAEPGFYPV
                     DRHASVQALITGQLILQMAYALCFWLARVTRRATLRSIEHLQRATRLAAQRDVQLAEL
                     RSDLDRALKIGGPGRFTGVAAGEWELGNVIGRGAMGEVYEATHAKTGDPAAVKLLRRE
                     LLADPRHIERFLREVRVAGAIDSPHVVRVLGASTPTDPMPYLAMEKLSGQTLSKLLRD
                     GRSLADPSIAALVDQIGAVLELARATGIVHRDIKPQNLFLTYDNTWKVLDFGVALLAE
                     TSGTLTRGAAIGTPAYMAPEQARGETVDHRADLYAFGAVLYRAITGRAPFAARDTPSL
                     LYAVTHDQPLRPSALAAVSPAVEQFLLLALAKSPEARLQTAAELVAAFQAARTGALPE
                     ELARRAAALPPWREQAPPPLGAN"
BASE COUNT         1473 a         2793 c         2998 g         1474 t
ORIGIN      
        1 gaaccgccgt gggcgacaat gcgctcgagc acgagtgcgg acgcggtggc cggatcgagc
       61 acgggcgcga tgtctatcgc ttcacgtacc tcgcgaagga tggctacggg cgctgggaaa
      121 tcgtgctgcg cgagcagcag attcgcgaca tcgcgggcgg cctcctcgac gaggtggacg
      181 cggtgcaact cgcggagggt acgcgcacgt cgcgcggtga tgcgttgatc gtgtggggcg
      241 aatacgacga ggacgcgttg cgcgttcgga cgaaaggcga cctcggcgtc gcgctcgacg
      301 cgctgcacac cgtcggcatc caggagccgt gcatggtgcg gctatggtcc tcgagcgatg
      361 accaagcgat cgcagtgttc aacgggcacg actgcgcgat ctacgtggtg gcgtccgagc
      421 atggttacgg ccgcagcgtg ggcgatccga cccggccgga tacattcgag ctgactgatt
      481 ttcgcgccgg tccggtatcg attccgtggg cggatgtcgt gccatggcgc gtcgcgcgcc
      541 cggcgctgat gatgtttgcc gagaaggggc agctcggcga caacgtgatt ctcgacggcg
      601 ccatcccgac gcagttcttg ttcctgtcgg acttcgatcg cgcgaccgag ctcggcacgc
      661 ggcgacagcc ggtcgctgat ccggcgcaat cgagcttgcc gcgcaaaacg ccgcacggcg
      721 aatgggccag gcggctggtc acgagcctgc tcgagatcca gttgatcgaa gtcgatacgc
      781 agatcctcga ggcggtgatc gcgcgtatcg cggtgctgct catcgcgcac gacgacgctc
      841 tcgacagccc ggacgcggcg cagaagctcg cgaaggagat cgagcgggtg aggggcgtcg
      901 gtgccctgtt cgcgaccggc ggcgacctcc agatcgcttt gcggcgcacg caggacccac
      961 caacgcaacc gaccgagtta ccgttcacct gatgcgttgg ctcgtcggtc tcgtgttcgt
     1021 ggctttcggg tgtggcgaca acctgccgcc acgcggcacg ttcgaggttg tcggccacag
     1081 cgatctcggc gcacgcggca tgaactcggc gctcgcgatc gcgggcgaca tcgcatacgt
     1141 cggctcgcgc atcgacaaga agccggtgct gatcgttgac atcaaagatc cggcgaatcc
     1201 gcacgtcgtc ggcgaaatcg gcgagcctgc cgagggcttg ccgggcatgt cctcgcgcga
     1261 gctgcgggcg acgacgaatc cgccgcggct cttcatcatg aacttggcgt gctcgccgga
     1321 tctgcacggc tgcagtggcg tgccaggtga gatcgagaac atcaaggagt acgacatctc
     1381 gaacccgctc gcgccgcagc tgctcggctc gcatggcgtc accggcagcg cgcttcgcca
     1441 gcgcagcccg cacgagttct ttctgtggca ggacccgtcg aatctcgagc gcgtgctgtt
     1501 gctcatcagt gcgccgggcg ttccgtcact cgagatcgtc gctgcgcaag acggtgatcc
     1561 gaagctgctc ttcgacttcg atccgtacag cgaaggcggc gtgacgcgcg gaggcgagaa
     1621 caacatcctg cactcggtgt cggcgagcga cgacgggcgc atgctgttcc tgtcgcatca
     1681 gatcgccggc ttgtacatcg ccgacgcgag cagcgtgatc gacatggtcg acccgctgca
     1741 gctcacgatg ctgacgaccg ccgacgccgc cgtgaagttt ggcgcgatgg gtccgcattc
     1801 ggccgtcgcg gtgccgggac gcaagctgct cgtcacgacc gaggaggtct acccgccacc
     1861 gttcggcgct ggctgtccgt ggggccacat gcggctcgtc gacatcgcga atcccgcgga
     1921 gccgaagcta cgcggcgagt acaagctgcc cgagaacgat ccgagctgca ccgcggccgg
     1981 gccgatggtg gcgttcaccg cgcataacgc gaccgtcatg aacaacatcg cgctcatcac
     2041 ctggtacgcg ggtggcctgc aggcgatcga gatttcggat cccgatgcac cgtatcagct
     2101 cggcgagttc aagccggagc cgcttccgag cgtcaccgtc gaggatccgg gactcagcgg
     2161 cagccgggtc gagatgtgga gctatccgat catcaaagat ggtctgatct acgtcgtcga
     2221 tgttcgcaac gggctctacg tgctgcgtta caacggctgg cgcgcggacg agatcctggc
     2281 gcggaagttc ctcgagggga actcgaacat cggttccttc acaccgcgta tctgacgcgg
     2341 catggatgtt gccaatcctc caccaggagg attgtcatga cagtcgcccg gtcagcattg
     2401 tttgcagccg cggtcgcgac agcggcattc ggatgtcatc gtcgtcatca acgaaatccc
     2461 gagcccgcgc tcgatctcga gacgagcaag ccagaccgta cggtcgagca gacgccgcca
     2521 tcccagtccg aggggttcgg caccgctgca ccgaacggtg gaggcgcgct cggcggtgcg
     2581 gtggtgtcga gcgcgcaggg ccgcaccaag atcatccaga tgcgtagccg cgcgaagcac
     2641 cactacaacg acacgagcca gaaccacgtc gaggaaactg cgccgggcga ccagccgacg
     2701 cagggcattg gcgacggcga cacctacgag cgcaatcgct gatcagcgcg tttggcgctg
     2761 gctcggtcga tcgtcaccgg atgcaggcga gccgctgcag gcgccggcca tcgacgcgat
     2821 ccattcgcgg atcaacgcga cgccttcctc gtggacgagg ttgcggccga gctccggcat
     2881 ccggatctcc ggttccgtcg agctgatgcg atagatcatg atcgactcgt cgggattgcc
     2941 cggcacgatg tcgtactgga ggttgcccga gccgcggccc gtcgcgaccg gcgctttgca
     3001 caggccgaac ttcgccggat cggtctccgc gtgatcgagg tagaggcccg acgtgcgcgc
     3061 agcgccgcgc gggttgtggc agaagccgca gttgatatcg agccacgcac gcgcacgttc
     3121 gtcgagcgtg ccgctcgtcg cgtcgaacgc gttcaccgcg cgcggccatt gatcgcgagg
     3181 cggggcatcg acaagcgcgc cgctatcgat catcgcctgc acgcggtcgg gtacgaggtt
     3241 gcgcaccttc gggcccaccg gcgtcacgat ctcgtcgtgc tcttcgtggc agagcttgca
     3301 ctggttctga ttcggcacgt tgtactggtt cgtgcgcatg ctgccgtcgt cgtgaatcca
     3361 ctcggcgtcg atgatggcgc ccgcgatcgc gaccttcgca tcgtcggtct cgttgccgta
     3421 cgtgtacgac gaaccgtgcc agcccttctc gccgtgaacg agcacgcgcg tctcgagcag
     3481 cgtgcggccg ttcgccggat cgcggcgatc gtggaggtag ctgaacgtct tcacgagcac
     3541 gctgccgacc ggcagatcga gcgggtcgtc cgcagtccat gtcgctggcg tgccgtcagg
     3601 cacccgcacg aaccgctgct tcgtcgtgta gtcggagaac agcggcgtcg tgaggtcgta
     3661 gggaatcacg ccggctgccg gcgtttgcga cgcgatgtcg tcgaacagct gccagctcga
     3721 cagccggctg cacatcttgc cgtcgatcgt gcactcctcg tcgccgccgc cgccgcatgc
     3781 ggccagtagc agcgctgcta cggccagtcg cttcacggca acgtcaccgc ggggagcgga
     3841 gcgagcgcgc agtcatgcgg cgccatgtct tcggtcggca cggtcgcgtc ggcgagcggc
     3901 cacttgagat cgatgaagtc agcgtcgccg ttgttcttga cgcagatgcg atcctcggga
     3961 ccgtacgtgc gcgccggatc gaggacgccg tcccacgcga cgtcgggcac gatgaacggg
     4021 ccgttcggcc ggatctcgcc gagcgccgag atcagcagcg cgccgagcat gccggtcggc
     4081 atgtcgctcg tgccggtcag cgtgttgtcg tggatgttga tcgcggtcgg gtactgatcg
     4141 taggtcgggt ctgtgaccac gatgcccgtc ggcacgtagc tgatcacgcc gacgttgatc
     4201 gacttgtgat cgctgacgcg gttgtcgaag atttcgacct ggtgcgcggc aagcactgcg
     4261 atgccggtac cggtcggcac gagaccgacg atgttgccag caggcgcgaa gttctccgtg
     4321 ttgttgccgc tcacgtcgtt ctcgtagacg cgcgtgatcg cgccgttcgg aacctcgagg
     4381 ccgggcaggt tgaacacgag gatgccgcca gtgttgcgcg tcgctgtgtt gtggaacacg
     4441 tccgcgtgca tcgagttctc gatctcgatg ccagcgacgt tgtcctcggc gaggttgtcg
     4501 tgcaggagga tcgtgtcgga ctgaccgacg tagacgccgg catccgacgc gccgcgcacg
     4561 acgctgttcg tgatcttcac gttcttgcac tgcaccgggt acaggccgta cgagccgttc
     4621 gtcgcgttcg ggccgcccgt ccactcgacg cgcaccttgt cgatcgtgac gtcggtcgag
     4681 ccgagaacct tgagcgcgtc gccgcgcgtg tcttcaattc cgatgtcgtg gatcgtgacc
     4741 gcgtctgacg tcacgagcag accctgcgcg ccggatgtct ggcttttgaa cgacagcgtc
     4801 gtcgcgtcca tacccgcgcc tttgatcgtc acgttgtcga cgtcgagcga caggtcggtg
     4861 cgcacgttga acgtgccggc accgaacgcg accgtgccac cgctctcgat ctcgatgagc
     4921 gccgtctgca catccgtgtc actcgcgccc tccgaaagac caatgcaggt acccgacacg
     4981 ccttcgcacg ggtcgtcact gccgcagcca gccacaacaa tcaaaagtga aagagctacg
     5041 cgacgcatcg cgggccagtc cgccagaaat tggtctgacc gtcaagggaa ttggtctgtc
     5101 caggtaatcg actgaagttc cggcggcata gccaatgcag tggattcctg gcgtgcggct
     5161 acccaagcaa ctccagcttg cgttgctcgt cgacgaagcg ccggacggcg acgactggct
     5221 gcacgaacaa aagttcgacg ggtaccgcat cgttgccgag ctcgagggtc gccggctgcg
     5281 gctgctgagc cgccgattca acgactggac cgcacagttt ccgaccgtcg tcggtgcggt
     5341 tgccgcgctc aaggtgaaga gcgtcctcct cgatggcgag gtcgcggtcg tgatgcccga
     5401 cggccgcacc agcttccagg cgctgcagaa cgcattcggt cgccgcgacg cgaacctcgc
     5461 gtacttcgtg ttcgatctgc tcgcgctcga tggcgaggac ctgacgaagc tcccgctcga
     5521 agagcgcaag caacggctcg cgaagttgat cggaaaacgg cagggcgtgc tgcgctactc
     5581 cgatcacgtc atcggcaatg gcaaggcgtt cttcgagctc gcgtgcaaga agggtctcga
     5641 aggcatcgtg tcgaagcggc gcgacgcgcc gtactcgccg ggccgcggca aaggctgggt
     5701 gaagaccaag tgcctcatgc ggcaggagct cgtgatcggc ggctacaccg atcccgaggg
     5761 cgctcgcacg cacatcggcg cgctgctcgt cggctattac gagggaggcg cgctgcgcta
     5821 cggcggcaag gtcggcaccg ggttcacgat gaaggacctc gtgaagatca agcaggcgct
     5881 gtccccgctc gaggtcgcga cgtcgccatt cgagcccgcg ccgccgcgcg cgtggaccgg
     5941 gccaaaccgg cactgggtct caccgaccat cgtcgccgag gtcgcgttct cggaatggac
     6001 gaacgacggg cgcctgcgcc atccgtcgtt tcagggcctg cgcgccgata agccgccgaa
     6061 cgagatcgtg cgcgagcaag cgacgcacgt gaatcgctag ggctacgatg cggcatgcgt
     6121 tgcgcgctcg tgttcctggt gttgctcgcc tgctcgtgca agaacgaagg ttcgcagcgg
     6181 gctccggtcg ggagtgcggc cgggagcgcg attgccaaac cgatcatccc ggatgacgtg
     6241 ctcgtcggtc ggtggttcgc tgcgatggag aaacagctcg atgccgacgc gcgtgcgctc
     6301 gcggtgcaaa agttcgcgag ccgggaggac gtcgagcacg cgctgatggg actgacacca
     6361 gcaggtttca aaccacgccc cgagttcttc caggcgttct acgccgaggc cggcgaaccg
     6421 acgacggtcg cgaagcggct cgctgactac gcgagcacac acggcgacga catggagcgc
     6481 cgcaccgacg caatgctgaa gcgtctcgag ccggcgatga agctcgccgc cgacaacgcg
     6541 gagcggcagt ttgcgcggtg atttccatgc tgcgtgatgt cacgtcaatt ctcgctgagc
     6601 gggcgtgggc gcgcggccga gcgacctggc ctcggcggct tttgcaggcg agatgtcgta
     6661 ggtcgtagcg gagcaggcac ttggaccgtc actccgtgtc atcgctgacg gagaaaatgg
     6721 gaaaggtgag ccggcctccc ggttgcacgt tctccacccg aggggcgatg agcgacacac
     6781 cgaagttttg gccaacgggt accgggcaat gggaccgact tgcgcgtctc gtcttggcgg
     6841 atgacgtcaa ggcggcgatg cagagcgagg gcgctgccgt gaacggaaca atgttcctcg
     6901 gcacgccgct cgatctcatc gaagagctcg agcgctgcac gcagctgtcg gcggcgagcg
     6961 tcgtgcttgt cggccgcttc gcgcacgacg aggtcgccga cgcgctgcgc gatatttatc
     7021 ccggtctcga agtccggctc gccgcttaat tcgcaccgag gggaggtgga gcttgctcgc
     7081 gccacggggg cagcgctgcc gcgcgccgtg cgagctcttc gggtaatgcg cccgtgcgcg
     7141 ccgcctggaa cgcggcgacg agctccgctg ccgtttgcag gcgcgcctcc ggagatttcg
     7201 cgagcgcgag caggagaaat tgctcgacgg caggcgaaac ggcggcgagc gctgacgggc
     7261 gcagcggctg gtcgtgggtg acggcgtaga gcagcgacgg cgtgtcgcgc gccgcaaacg
     7321 gcgcacggcc ggtgatcgcg cgatagagaa ccgcaccgaa cgcatagagg tcggcgcggt
     7381 ggtcgacggt ttcgccgcgc gcttgctcgg gtgccatgta cgcgggcgtg ccgatcgcgg
     7441 caccgcgcgt gagcgtgccc gacgtttcgg cgagcagcgc gacaccgaag tcgagcacct
     7501 tccacgtgtt gtcgtaggtg aggaacaggt tctgcggctt gatgtcgcga tgcacgatgc
     7561 cggtcgcgcg cgcgagctcg agcactgcac cgatctgatc gactagcgca gcgatggacg
     7621 gatcggcgag cgagcgcccg tcgcgcagca gcttcgacag cgtctgaccc gagagcttct
     7681 ccatcgcgag gtacggcatc ggatcggtcg gcgtcgatgc accgagcacg cggacgacgt
     7741 gcggcgaatc gatcgcaccg gcaacgcgca cttcgcgcaa gaagcgttcg atatggcgcg
     7801 ggtcggcgag cagctcgcgg cgcagcagct tgactgccgc cggatcgccg gtcttcgcgt
     7861 gggtcgcctc gtacacctcg cccatcgcgc cgcgtccgat cacgttgccg agctcccact
     7921 cgccggcggc gacgccggtg aaccggccgg ggccgccgat cttgagcgcg cgatcgagat
     7981 cgctgcgtag ctcggcaagc tggacgtcgc gttgcgcggc gagtcgggtc gcacgttgca
     8041 gatgctcgat cgagcgcaac gtggcgcgcc gagtgacgcg cgcgagccag aaacacagcg
     8101 cgtacgccat ctgcagaatg agctgcccgg tgatcaacgc ctgcaccgac gcgtggcgat
     8161 cgacgggata gaagcccggc tcggcgaaca caccggcgat cacgagcatc gatgcgacga
     8221 agtgcgcgat cgccgcgatg gcatagatcg cgatcgcgct cagcaggttc tcggtgcggc
     8281 agaagatgta gatgcagagc gtgaggccga ccggcgcggc ggagaacgtg ccggtgtaga
     8341 ggatgccgag gacgccgcag ctcaccacta cgagtgcgag cagcgtcatc gtcgtcgtcc
     8401 ggtagcgcgt ggcgtcgcgt aggtcgcgat acacccacaa gctgccgagg acaccgatgc
     8461 cgagcagtgc gagcagcgcg tgggcgatgc gccgatcccc gggcgcgatc agcactgcga
     8521 ctgcgacgac gatgccggcg atccacccga cgccgagaag gctgcgcgtg cgcttgatct
     8581 cctcggcgcg cagcgcatct gctgcgttcg tcatcaccga gatcgagccg cgggtcgtca
     8641 tatcggctcc cacgggtgtt tgcggatcaa cgcatcgccg cggcggcgca gctttggatc
     8701 gagactgccg gcgagcgcat cctcgagcgc gtcggcga
//
LOCUS       DASUFX010000167        12718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1833419, whole genome shotgun sequence.
ACCESSION   DASUFX010000167 DASUFX010000000
VERSION     DASUFX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833419"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2637
                     /locus_tag="VFV99_06690"
     CDS             <1..2637
                     /locus_tag="VFV99_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059027.1"
                     /translation="QAGRLLKMAAAADGEDEKVLAMYERVARNGGDQELLLDFLERRA
                     AHSSATPKQIREAVDLAIELGQDERAEALLVRAVAAARDTVDGIASAPWAVMALAERR
                     LAANDLVQARDLTYEIAPIATTEELASQVDGLAMRVATRALAHRKIEIAAEVYEFLRE
                     RSPSDRAVWQPLMTIYRELGDGDRLASVVSSTLPNLVTAGERNALRIEHTKFLIENLK
                     RHHDALDVLRDALKDDPDNLEAAELYEGTLKELGDDDGIAEFLWSRFEDAQRRGHRDS
                     TVDVALRLGDLLEKQESPDAVRVYRAALIVAPDDREILRRVVAQLGTHDNPREGALLM
                     ERLLAVETPERAPELAGKLATMWEAAGDRRGVQRTLELAHKSAPDDKAIHDRLEKWYR
                     DNEQWAELANLMTQDAERLGDTPAVERLREAAKVYSEFLGQPLKAAEVLRKARQRAPK
                     NVELVTDHAAALASAGELDAAQRAVGEALATVEGAQRTSLLLLRASFRQQLGDDSSAV
                     QDLTEAYELDKERVAEPIVMGLERLRGRAERDGDLPTERMATLKLAQLLTQHGDFERG
                     RGFLVGWIERDPRDAEPLYMLCDLDESIEHWDGVAAAATRLAYVTEGEAQVTAAMRAS
                     DACAKAGRPGEAVPVLELVFQQQPGVDALRDKLRDVYEAAGEYRQLAGILTADADHGS
                     DPATRYANYKRAAELLLYQLEDAVAAQVPAQKALELQPDDHGALMLNIDVLITSGQLE
                     DAGRTLEAAIAAQKKRTPELAQQQQRMGRVCAMMGDKEGQLNWLKKAFDVDRKNAEVA
                     AELAQLATELGDYELALKPLRAITLMDNPSPVTRPMALLWEAKIEHARGNRAKAELWA
                     KKALREDPQFADAQQFLDELGG"
     gene            complement(2717..3223)
                     /locus_tag="VFV99_06695"
     CDS             complement(2717..3223)
                     /locus_tag="VFV99_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059028.1"
                     /translation="MKALITLLIIGTSSVALAQPAIVDHRNDYAFEDRDHRFDERDRD
                     RRLDLDRGYARAFGYRMRPVTLASNVSLMRQRNRDQRPLLIDIDSRMGTFKTLRLDRV
                     DGRAYINDVVVMLADGHSIRYDVNKMMSLRSPSITIDVGQRAITGIYVYGMTERGRAS
                     FNVIGLRR"
     gene            complement(3235..3420)
                     /locus_tag="VFV99_06700"
     CDS             complement(3235..3420)
                     /locus_tag="VFV99_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059029.1"
                     /translation="MLIVCSSALAFADPPADKDKNERNWTIHAAKMYATKDGDNKPVV
                     TTPGVFVTAQRDPQDKK"
     gene            3528..4313
                     /locus_tag="VFV99_06705"
     CDS             3528..4313
                     /locus_tag="VFV99_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5059030.1"
                     /translation="MSEAVRYHADGMVGVITLDRPDNRNSMTPELLDAFAAASARARA
                     DTSIRCLVVTGTGPCFSSGADFKSTLQRGSSEALAPHERSYAMYVPFLSLLDIDVPIV
                     GALNGHTVGGGFGLALVCDIRVGATEAKYGANFVTLGLAPGMSISYLLPRLIGMAKAN
                     ELLLTGRLVMGPEAEALGILNKAVPAAQVMPEAMTIARTIAGNAPLAVRATKAAIRRG
                     LALTVREAAREEAVAQAETLATADCAEGIAALLAKRPPVFTGR"
     gene            complement(4310..4735)
                     /locus_tag="VFV99_06710"
     CDS             complement(4310..4735)
                     /locus_tag="VFV99_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059031.1"
                     /translation="MRWLTIALVATACGGRGADHTAPSYEPEPVVPIRVEWKVEQGAG
                     NEVNVALVVDGTSLAVGSLDAATEHEAGTPSTCALRAASQRRTEIVCGDTNGFAADLV
                     GNELLVTYVANEQPSEVKRVPVNGNVLNVKMLVLPGSKL"
     gene            complement(4723..5730)
                     /locus_tag="VFV99_06715"
     CDS             complement(4723..5730)
                     /locus_tag="VFV99_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059032.1"
                     /translation="MRAFLIALCACGGGAATISSPTKTALPKTKPAVETRAPVDVIDV
                     LAQPVAESSWIAPGPAQLLLGGASVQALEGAPRLEVTVLEEQGNDIRVGVRLDQARFA
                     LWMSRSRLLAIVGHDTRLTVPGVYTVGVDPMQVVLHAGAQVQRLAKKNGNTQVRYVGA
                     LEVEGWIPDDALTDRWPAGRTNRGRVPNGRRPLMLMSGAVIRVEPKWAGEQLAITNQG
                     YFVDEIKAVDEAWSEVSYEDSDLFVHGYVSKRDPPGRTHRRKAPEQTVPLVPNATVAD
                     GTCLYAGDEEIGFVVGDQQLIVDKGTRLGMFTVTIDTPWGPLAFEAKGATEQELATCG
                     G"
     gene            5895..6941
                     /locus_tag="VFV99_06720"
     CDS             5895..6941
                     /locus_tag="VFV99_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5059033.1"
                     /translation="MEIGVITNPNSRKNRNRPQRVERLQRIVGRMGEVHQTESIDSIK
                     PVLRDFLRKRARYWVADGGDGALHWMLRMGMEVLQEDEFAESSVTLPLTLPTKGGTID
                     FVANNVGIQGDAEGILATLRRHLEHGATIEETEVDSMVIDGIQLVDGQEVSFRTYGFA
                     SAAGGVGQRFYSKYYQAADPNPRTIVKVVANTVTSAPIAMSPLRKLPLGHLSTYAKEV
                     FAPTGCKITLDGMVLPGTQFTGVHIASMSINLGNVLRFFGKADQPGVMNALVGTPTAW
                     GIIRNLPRMMRGQEMVGRDVLDRPCREMKLVAHGEELLEPIIDGEYYRNVKQITFRVG
                     PRVRIPKVVAPKAA"
     gene            complement(6963..7289)
                     /locus_tag="VFV99_06725"
     CDS             complement(6963..7289)
                     /locus_tag="VFV99_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059034.1"
                     /translation="MSLRVVLLLIAFAFSCGGETMRVKKGEPQTAREKMLAEQKAHPD
                     NDDSGTGKKWTGWRYQGDRKDCFFLVGRKCYKTQNAACQAAHCRAPTKCTTEGAGPAT
                     MKCEKP"
     gene            7317..7919
                     /locus_tag="VFV99_06730"
     CDS             7317..7919
                     /locus_tag="VFV99_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059035.1"
                     /translation="MPDDDRAVMERLAALEAEVKADADAQRARKEAAIAKVREERAKH
                     QAERDELRSRQAALVTRKPREESRPRRDEEFDHADGLGGALELAGKANRVRGELAKKP
                     GKGDKSWIKSGIASTALGPIGWLYAGSLREAVPAATAWVAILFLLKFLPSLLLMPAML
                     VALPLSGLAGVLYALSYNRHGGRQRLFGDKEKKQLPSGDR"
     gene            8023..8871
                     /locus_tag="VFV99_06735"
     CDS             8023..8871
                     /locus_tag="VFV99_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059036.1"
                     /translation="MGRLLALLALLISTTANAVADTSPPPRVAIVPGIAVNLDPARVD
                     ALSQDLAQALMSELVVDAVGGLEVRRLLPADGLPPDCVTTPACTADVAKRTNASQLLF
                     VVMVDSGSGGSIQIDTTWVDAASAKTASRPAIDLTSTADAKAKFTTAAHDLLPDAALR
                     PKPTNGGGGIGVNTKMTEGVPRHFTTPAKITAAVGVVGLGAGIAFGLTTRSRYNACED
                     QLGTCTQDKRNSIRYLGVAADAGFVVAIGGAIATVVLYMQSAQEPHLIVSPTTDGATV
                     SAVGRF"
     gene            8871..10679
                     /locus_tag="VFV99_06740"
     CDS             8871..10679
                     /locus_tag="VFV99_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059037.1"
                     /translation="MGIESSAEKAIERLTGEVLANRYQIESKIGEGAMGAVYRAKHVK
                     VGRAFAVKVLHPRLLEDLKTTQRFEREAELAGRLRHPNVIGVVDVGETPDGMRYMVMD
                     FAAGPDLATLLNEAPFPPQRIIHLVRQLLEGLYHAHESGLIHRDFKPENVIVEKDTHG
                     SEVPRIVDFGIAILREGGDSTDGPGRLTTNGLVLGTPHYMAPEQAVADPIDHRIDLFA
                     LGIMIYEMLSGKLPFDGSGVEVARANLLMDPPPIAERVPYLEVDPLLEAYANRLMAKK
                     RDRRPATAKAARELLDLIERDRPKAAAALGVPLETAARGPQTTQPQAPVRQHSGNDTF
                     PPPLASAPTEKQAPSMRVPMAVAASAHEPHITTAQTNAKPSRAPIIIAAIAGLGVLGG
                     AAFVMLSGTKKHDDKVAVAAEPAKAEPAPPVEVTPAPVETKPAPVETKPAPVETKPDE
                     MKPAEFVAAEPGSAAEPEPKTQTAKVTPKTTKTTKTTKPQTTKTETKAQPTLPPPNNV
                     TTKVEHPVETKAEHPVQTKAEPKQATSSMVASQYQAVGSQLKALQDAKGVDAVSDLWS
                     RYRLIRLVDAMSTQPKRDEAAALLGKLASEIAARKK"
     gene            complement(10880..12433)
                     /locus_tag="VFV99_06745"
     CDS             complement(10880..12433)
                     /locus_tag="VFV99_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059038.1"
                     /translation="MRQYVAMLMPMVVGSGCSLLYNPSNLGKANPDASDAPMIDAEMT
                     ADADIDAPPLADANPGMLTLLDVTPKVLLEGQGQFGSRPAVLVIKGHHIVSDNLSVAI
                     TPSTGITVGATTVAMNGDYIAVEVSVAIDTNANAGMTPLTITVMQNGGPGGGVALTNK
                     LSLQNLPQFPAGTSIATGSLAELYSDVNTGNVTFTGTARASVRSVSRIKMGNITANGG
                     NGSTNGAGGAGPGACAGGAPASNAPCLQSDGGGVGAGGDSGGGGGGFAVAGNPGVTGD
                     AGTGGAGGGAHGDNQIISFAAQGAGGGGGGKGALSNSGGGGGGGGTIELTAGGNIEVG
                     TIDAKGGAGGAGSSVLSLGASGGGGGGSGGTVVLRSDTGTIVSGAIAATPGGGGASGG
                     TGAGAGGAGAPGRVRVDVASGSLPSSNPSLHRGPAFVNVPTVVTTNKVMLSMTGANAD
                     VVNAYVIPDDGMVHLGEPMGMMFNNGMLSFQGVLLPGYNKLCVTLEPGMRGHADTLAD
                     KCIELAYLP"
BASE COUNT         2224 a         4304 c         4160 g         2030 t
ORIGIN      
        1 caagcgggcc gtctgctcaa gatggccgcg gctgccgacg gtgaagacga gaaggtgctc
       61 gccatgtacg agcgcgtcgc gcgcaacggt ggcgatcagg agctgctgct cgacttcctc
      121 gagcgccgcg ctgcgcactc gagtgccacg ccgaagcaga tccgcgaggc ggtcgatctc
      181 gccatcgagc tcggccaaga cgaacgtgcc gaggcgttgc tcgtccgtgc cgtcgccgct
      241 gcgcgcgata ccgtcgacgg tatcgcatcg gcgccgtggg ccgtcatggc gcttgccgag
      301 cgccggctcg ccgcaaacga tctcgtccag gcgcgggacc tgacctacga aatcgcgccg
      361 atcgcgacaa ccgaggaact tgcctcgcaa gtcgatggtc tcgcgatgcg ggtggcgacg
      421 cgtgcgctcg cccatcgcaa gatcgaaatc gcggccgagg tgtacgagtt cttgcgcgag
      481 cgcagcccga gcgaccgcgc ggtgtggcag ccgctgatga cgatctaccg cgagctcggc
      541 gatggcgatc gcctcgcaag cgtcgtgtcg tcgacgttgc cgaacctcgt gaccgcgggc
      601 gagcgcaacg cgctccgcat cgagcacacg aagttcctga tcgagaacct caagcgccat
      661 cacgacgcgc tcgacgtgtt acgtgacgcg ctcaaggacg atccggacaa cctcgaagcg
      721 gccgagctct acgaaggcac gctcaaggag ctcggtgatg acgatggcat cgccgagttc
      781 ttgtggtcgc gcttcgaaga cgcgcagcgt cgtggtcatc gcgactcgac ggtcgacgtc
      841 gcactccggc tcggtgatct gctcgagaag caggaatcgc cggacgcggt tcgcgtctat
      901 cgtgcggcgt tgatcgtcgc gccggacgat cgcgagatcc tgcgtcgcgt cgtcgcgcag
      961 ctcggcacgc acgacaaccc gcgcgaaggc gcgttgctca tggagcgctt gctcgcggtc
     1021 gaaacgcccg agcgcgcccc cgagctcgcc ggcaagctcg cgacgatgtg ggaagccgcc
     1081 ggcgatcgcc gcggcgtcca gcgcacgctc gagctcgcgc acaagtcggc gcccgacgac
     1141 aaggcgatcc acgatcgcct cgagaagtgg tatcgcgata acgagcagtg ggccgagctc
     1201 gcgaatctga tgacgcagga cgcggagcgc ctgggcgata cgccagcggt cgagcgccta
     1261 cgcgaagccg cgaaggtgta ctcggagttc ctcggtcagc cgctcaaggc cgccgaggtg
     1321 ttgcgcaagg cacgccagcg cgcgccgaag aacgtcgagc tcgtcaccga tcacgccgcg
     1381 gcactcgcct cggccggtga gctcgatgcc gcgcaacgcg ccgtcggcga agcgctcgcc
     1441 accgtcgagg gtgcgcagcg cacgtcgctg ttgctcctcc gcgcgtcgtt ccgccagcag
     1501 ctcggcgacg attcgtcggc cgtgcaggat ctcaccgagg cgtacgagct cgacaaggag
     1561 cgcgtcgccg agccgatcgt catgggcctc gagcggctgc gtggccgcgc cgagcgcgac
     1621 ggcgacctgc cgaccgagcg catggcgacg ctcaagctcg ctcagctgct cacgcagcac
     1681 ggcgacttcg agcgcggccg cggcttcctc gtcgggtgga tcgaacgcga cccgcgtgac
     1741 gccgaaccgc tgtacatgct gtgcgatctc gacgagtcga tcgagcactg ggacggcgtg
     1801 gccgccgctg caacgcgtct cgcgtacgtg accgagggcg aagcgcaggt cacggccgcg
     1861 atgcgtgcga gcgatgcatg cgcgaaggcc ggtcgcccgg gcgaagcggt gccggtgctc
     1921 gagctggtgt tccagcagca gcccggcgtc gatgcgctgc gcgataaact gcgcgatgtc
     1981 tacgaagcag ccggtgaata ccggcagctc gcaggcattc tgaccgccga cgccgatcac
     2041 ggcagcgatc cggccacccg ctacgcgaac tacaagcgtg ccgccgagtt gctcttgtat
     2101 cagctcgagg acgcagtcgc ggcgcaggtc ccggcgcaga aggcgctcga gctgcagccc
     2161 gatgatcacg gtgcgttgat gctcaacatc gacgtgttga tcacgagcgg ccagctcgaa
     2221 gatgccggcc gcacgctcga agcggcgatc gccgcccaga agaagcgcac gccggagctc
     2281 gcgcagcagc agcagcgcat gggccgcgtg tgcgcgatga tgggcgacaa ggaaggccag
     2341 ctgaactggc tgaagaaggc gttcgacgtc gaccgcaaga acgccgaggt tgcggccgag
     2401 ctggctcagc tcgcgaccga gctcggcgac tacgagctcg cgctcaagcc gctgcgcgcg
     2461 atcacgctga tggacaaccc gtcgccggtg acccggccga tggcgctgct ctgggaagcg
     2521 aagatcgagc acgcgcgcgg caaccgcgcg aaggccgagc tgtgggcgaa gaaggcgctg
     2581 cgcgaagatc cgcagttcgc agacgcacag cagttcctcg acgagctcgg cggttagtca
     2641 ggccgctcgg gcaaagagaa accgccaggc tgatgcgcgc agcctggcgg ttttgttttc
     2701 ggcaacgtct ttggggttat cgacgaaggc cgatcacatt gaagctggca cgaccacgct
     2761 cggtcatgcc gtacacgtag atgccggtga tcgcgcgctg gccgacatcg atcgtgatcg
     2821 acggcgagcg caacgacatc atcttgttca cgtcgtagcg aatcgagtgg ccgtccgcga
     2881 gcatgacgac gacgtcattg atgtacgcgc ggccgtcgac gcggtcgagt cgcagcgtct
     2941 tgaacgtgcc catgcgcgaa tcgatgtcga tcaagagcgg gcgttgatcg cgattgcgct
     3001 ggcgcatcag cgagacattc gacgcgagcg tcaccggacg catgcggtac ccgaatgcgc
     3061 gggcgtagcc gcgatcgaga tcaaggcgac gatcgcggtc acgttcgtcg aagcggtggt
     3121 cgcggtcctc gaacgcgtag tcgttgcgat gatcgacaat cgctggctgc gcaagcgcaa
     3181 ccgacgacgt gccgatgatg agcaaagtga tgagtgcctt catgcttggt ctccttactt
     3241 cttgtcttgc gggtcgcgtt gcgcggtaac gaacacgccc ggcgtggtga cgaccggttt
     3301 gttgtcgccg tccttcgtcg cgtacatctt ggcggcgtga atcgtccaat tgcgctcgtt
     3361 cttgtccttg tcggcgggtg gatccgcaaa ggcgagcgcc gaggagcaaa cgatcaacac
     3421 ggcaatgatc ttcgaaagcg acatgccgta gagacgcggt gcttcacgta gcgattcaag
     3481 agtgctgtgt gaacgaatgt gaacgtgaac gcggtaactt cccgagcatg tctgaggcag
     3541 ttcgctatca cgccgatgga atggttggcg tcatcacgct cgatcgtccc gacaatcgca
     3601 acagcatgac gccggagctg ctcgatgcat tcgctgcggc gagcgcgcgt gcacgcgccg
     3661 acacgtcgat ccgctgcctg gtcgtcaccg gcaccggtcc gtgtttctcg tccggcgccg
     3721 acttcaagtc gacgctgcaa cgcggctcca gcgaagcgct cgcgccacac gaacgctcgt
     3781 acgcgatgta cgtgccgttc ttgtcgctgc tcgatatcga cgtcccgatc gtcggtgcgc
     3841 tcaatggcca cacggtcggc ggcggcttcg gcctcgcgct cgtctgcgat attcgcgtcg
     3901 gcgcaaccga ggcgaagtac ggcgcgaatt tcgtcacgct cggcctcgcg cccggcatgt
     3961 cgatcagcta cctgctgccg cgcctcatcg gcatggcgaa ggccaacgag ctcctgctga
     4021 ccggcagact cgtcatgggc cccgaagccg aggcgctcgg catcctcaac aaggccgtgc
     4081 ctgcggccca ggtgatgccc gaagcgatga cgatcgcgag gacgatcgcc ggcaacgcgc
     4141 cactcgctgt acgcgcgacg aaggcagcga ttcgccgcgg ccttgcactc accgtacgcg
     4201 aagccgcgcg tgaagaagcg gtcgcgcagg ccgagacgct cgcgaccgcc gactgcgcgg
     4261 agggcatcgc tgccctgctc gcgaagcgcc cgccggtctt caccggccgt tagagcttgc
     4321 tgcccggcaa cacgagcatc ttcacgttga gcacgttgcc gttgaccggc acgcgcttca
     4381 cctcgctcgg ttgttcgttc gcaacgtagg tgacaagcag ctcgttgccg acgaggtcgg
     4441 ctgcaaatcc gttcgtgtca ccgcacacga tctcggtccg gcgctggctc gctgcgcgca
     4501 atgcgcacgt cgacggcgtc cctgcttcgt gctcggttgc ggcgtcgagc gagccgactg
     4561 cgagcgaagt cccgtcgacg acgagcgcca cgttgacctc gttgccggcg ccctgctcga
     4621 ccttccactc gacacggatc ggcaccaccg gctcgggctc gtacgacggt gccgtgtgat
     4681 cagcgccgcg cccaccgcat gcagtcgcga cgagcgcgat cgtcagccac cgcatgttgc
     4741 gagctcctgc tccgtcgcgc cttttgcctc gaatgcgagc ggtccccacg gcgtatcgat
     4801 cgtaaccgtg aacatgccga ggcgagtccc tttgtcgacg atcagctgtt gatcgccgac
     4861 gacgaatccg atctcctcgt cgcccgcgta caggcacgtg ccgtcggcaa ccgtcgcgtt
     4921 cggcacgagc ggcaccgttt gctccggggc tttccgccga tgcgtacgcc ctggcggatc
     4981 gcgcttcgac acgtagccgt gcacgaacag atcggagtct tcgtagctca cctcggacca
     5041 cgcttcgtcg accgctttga tctcgtcgac gaagtagccc tgattcgtga tcgcgagctg
     5101 ctcacctgcc catttcggtt cgacgcgaat caccgcaccc gacatcagca tgagtggcct
     5161 gcgtccgtta ggtacgcggc cacgattcgt gcggcctgca ggccagcgat cggtcagggc
     5221 atcgtccgga atccagcctt cgacctcgag tgcaccgacg taccgaacct gcgtgttgcc
     5281 gttcttcttc gcgagccgtt gtacctgtgc gcccgcgtgc agcacgacct gcatcggatc
     5341 gacgccgacc gtgtagacac cgggcaccgt cagccgcgtg tcgtggccga cgatcgcgag
     5401 caatcgactg cgcgacatcc acagcgcgaa ccgcgcttga tcgagccgca cgccgacgcg
     5461 gatgtcattg ccttgctcct cgagcacggt gacttcgagc cgcggcgcac cctcgagcgc
     5521 ctggacactg gcgccaccga ggaggagctg ggctggacca ggcgcgatcc acgacgactc
     5581 ggcgaccggc tgcgcgagca catcgatcac gtcgaccgga gcgcgcgttt cgaccgcggg
     5641 cttcgtcttc ggcaatgcgg ttttcgtcgg tgacgaaatc gtcgcagcgc cgccgccaca
     5701 cgcacagagc gcgatgagga atgccctcat gtgccggatg aaattgccac gattccgtcg
     5761 cgccgccgcc accgttatcc actggacgcg taatgggcta cgcaccaagc caggctttgc
     5821 ctactgtcat cacgtgatat tcgtcgctta cgacgcggcg cgtctcgcac agctgttgca
     5881 accagggggg cgcgatggaa atcggcgtca tcaccaaccc aaatagccgt aagaaccgca
     5941 accgtcccca gcgggtggag cggcttcagc ggatcgtggg tcggatgggc gaagtgcacc
     6001 agaccgagtc gatcgactcg atcaagccgg tgctcagaga ctttctgcgt aagcgcgccc
     6061 gctactgggt cgcggacggc ggcgacggtg ctctgcactg gatgctccgc atgggcatgg
     6121 aagtcctcca ggaggacgag ttcgccgaga gctccgtgac gcttcccctc acgctcccga
     6181 ccaagggcgg caccatcgat ttcgtcgcga acaacgtcgg tatccagggc gacgcggagg
     6241 gcatcctcgc gacgctccgc cgccatctcg agcacggcgc cacgatcgaa gagacggaag
     6301 tcgactcgat ggtgatcgac ggcatccagc tggtggacgg ccaggaggtt tcgttccgca
     6361 cgtacggctt cgcgtcggcg gcgggcggtg tcggccagcg cttctactcg aagtactacc
     6421 aggcagcgga tccgaacccg cgcacgatcg tgaaggtcgt cgcgaacacg gtgacgtccg
     6481 cgccgatcgc gatgtcgccg ctgcgtaagc tgccgctcgg tcacctgagc acctacgcga
     6541 aggaagtgtt cgcgccgacc ggctgcaaga tcacgctcga cggcatggtg ctgccgggca
     6601 cgcagttcac cggcgtgcac atcgcgtcga tgtcgatcaa cctcggcaac gtgctccgct
     6661 tcttcggcaa ggccgaccag ccgggcgtga tgaacgcgct cgtcggtacg ccgaccgcgt
     6721 ggggcatcat tcgcaacctg ccgcgcatga tgcgtggcca ggagatggtc ggccgcgacg
     6781 tgctcgaccg tccatgccgc gagatgaagc tcgtcgccca cggcgaagag ctcctcgagc
     6841 cgatcatcga cggcgagtac taccgcaacg tcaagcagat cacgttccgc gtcggcccgc
     6901 gcgtccgcat cccgaaggtc gtcgcgccga aggccgcgta ggtaaccgta gctagcgcgc
     6961 agctacggct tctcgcactt catcgtcgcc ggaccggcac cctcggtggt gcacttcgtc
     7021 ggcgctctgc aatgcgctgc ctggcaggca gcgttctggg tcttgtagca cttgcgaccg
     7081 acgaggaaga agcagtcctt acggtctccc tggtagcgcc agcccgtcca tttcttgccc
     7141 gtgcccgaat cgtcgttgtc gggatgcgct ttctgctcgg cgagcatctt ctcgcgcgca
     7201 gtctgcggct cgcctttctt gacgcgcatg gtctcgccgc cacaggagaa cgcgaaggcg
     7261 atcaggagca ggacgacgcg cagcgacatt tgccgagatc atgccatagt ccagacgtgc
     7321 ccgacgacga tcgcgcagtg atggagcggc tcgctgcgct cgaggcggag gtcaaggccg
     7381 acgccgatgc gcaacgggcg cgcaaagaag ccgcgatcgc gaaggtccgc gaagaacgcg
     7441 ccaaacacca ggccgagcgc gacgagctac gctcgcgcca ggctgcgctc gtgacgcgta
     7501 aaccgcgcga ggagagccgg ccgcggcgcg atgaggaatt cgatcacgcc gatggtctcg
     7561 gcggcgcact cgagctggct ggcaaggcga accgcgtccg cggcgagctt gcgaaaaagc
     7621 ctggcaaggg cgacaagtcg tggatcaagt cgggcatcgc gtcgaccgcg ctcggcccga
     7681 tcggctggct ctacgctggc tcgctgcgcg aagcagtgcc ggccgcgacc gcgtgggtcg
     7741 ccattctgtt cctgctcaag ttcctgccat cgctgctgct gatgccggcg atgctcgtcg
     7801 cgctgccgct gtcgggcctc gcaggcgtgc tgtacgccct gtcgtacaat cgccatggcg
     7861 gacgccagcg cctatttggt gacaaggaaa agaaacagct tcccagcggc gaccgttagc
     7921 tgcgcgctag ggcacactac ccaacggaag actgtcgtga gggatgaccc tcgacgaaga
     7981 gtgtcgctag atcacccttc ctggggccgt taagatcaac tcatggggcg actgcttgcg
     8041 ctgctagcgc tgctgatttc gacgactgcg aacgcggtcg cagacaccag tccgccaccg
     8101 cgcgtcgcga tcgttcccgg catcgcggtc aacctcgatc ccgcgcgcgt cgatgcgctg
     8161 agccaggatc tcgcccaggc gctgatgagc gagctcgtcg tcgacgcggt cggtggcctc
     8221 gaagtgcgcc ggctgttgcc cgccgatggt ctgccgccgg actgcgtgac gacgcccgcg
     8281 tgcaccgccg atgtcgcgaa gcggacgaac gcgtcgcagt tgctgttcgt cgtcatggtc
     8341 gatagcggct cgggtggctc gatccagatc gacaccacgt gggtcgatgc ggcgtcggcg
     8401 aagaccgcgt cgcggcctgc gatcgatctc acgtcgactg ccgacgccaa ggcgaagttc
     8461 accaccgccg cccacgactt gcttcccgac gctgcgctgc gcccgaaacc gaccaatggc
     8521 ggcggtggca tcggggtcaa taccaagatg accgaagggg tgccgcgcca cttcacgacg
     8581 ccggccaaga tcacggcggc cgtcggtgtc gtcgggctcg gcgccggcat cgcgttcggc
     8641 ctcacgacgc gctcgcgtta caacgcgtgc gaggaccagc tcggcacctg cacgcaggac
     8701 aaacgcaatt cgattcgcta cctcggggtg gcggcagatg ccggcttcgt cgtggcgatc
     8761 ggcggcgcga tcgcgaccgt ggtgctctac atgcaatcgg cgcaggagcc gcacctgatc
     8821 gtgtcaccga caaccgatgg cgcgacggtg tcggccgtcg ggagattttg atgggcatcg
     8881 agtcgagcgc ggaaaaagcg atcgaacgcc tcaccggcga ggtgctcgcg aaccgttacc
     8941 agatcgaatc gaagatcggc gaaggcgcga tgggcgccgt gtaccgcgcg aagcacgtca
     9001 aagtcggtcg cgcgttcgcg gtcaaggtgc tgcacccgcg cctgctcgag gatctcaaga
     9061 cgacgcagcg cttcgagcgc gaagccgagc ttgcgggtcg gctccggcat ccgaacgtga
     9121 tcggtgtcgt cgacgtcggc gaaacgcccg acggcatgcg ctacatggtg atggacttcg
     9181 ccgcgggtcc ggacctcgcg acactgctca acgaggcacc gttccccccg cagcgcatca
     9241 tccacctcgt ccgccaactc ctcgaaggcc tctatcacgc gcacgaatcg ggcctgatcc
     9301 atcgcgactt caagcccgag aacgtgatcg tcgagaaaga cacgcacggc agtgaagtgc
     9361 cgcgcatcgt cgatttcggc atcgcgattc tgcgcgaggg cggcgactcg accgacgggc
     9421 ccggccgcct gacgacgaac ggcctcgtgc tcggcacgcc gcactacatg gcgcccgagc
     9481 aagccgtcgc cgatccgatc gatcaccgca tcgacctgtt cgcgctcggc atcatgatct
     9541 acgagatgct gtcggggaag ctgccgttcg acggcagcgg cgtcgaggtc gcgcgtgcaa
     9601 acctattgat ggatccgccg ccgatcgcgg agcgcgtgcc gtacctcgag gttgatccgc
     9661 tgctcgaggc gtacgcaaac cggttgatgg cgaagaagcg cgatcgccgc ccggcaacgg
     9721 cgaaggccgc acgcgagctg ctcgatctca tcgaacgcga tcgtccaaag gccgccgccg
     9781 cactcggcgt tccgctcgaa actgccgcgc gcggaccgca gaccacgcag ccacaggcgc
     9841 cggttcgcca gcacagcggc aacgacacgt tcccgccgcc gctcgcctcg gcaccgacgg
     9901 agaagcaagc gccgtcgatg cgcgtgccga tggccgtcgc tgctagcgcg cacgagccgc
     9961 atatcacgac ggcgcaaacg aacgcgaagc caagccgtgc gccgatcatc attgccgcga
    10021 tcgccggcct cggcgtgctc ggtggtgcgg cgttcgtgat gctgagcggc accaagaaac
    10081 acgacgacaa ggtcgcggtc gccgccgagc ctgcgaaagc cgagccggcg ccacccgtcg
    10141 aggtgacgcc agcgcccgtc gagacgaagc cagcgcccgt cgagacgaag ccagcgccgg
    10201 tcgagacgaa gccggacgag atgaagccgg ccgagttcgt cgcggccgag cccggcagcg
    10261 ccgccgagcc cgagccgaag acgcagaccg cgaaggtcac gccgaagacg acaaagacga
    10321 cgaagacgac aaagccgcag acaacgaaga ccgagacgaa ggcacagccg acgctgccgc
    10381 cgccgaacaa cgtcacgacg aaggtcgagc atccggtcga gacgaaggca gagcatcctg
    10441 tccagacgaa ggcagagccg aagcaagcca cgtcgtcgat ggtcgcgagc cagtatcaag
    10501 cggtcggcag ccagctcaag gcgctgcagg acgcgaaggg cgtcgacgcg gtgagcgacc
    10561 tgtggtcgcg atatcgcctg atccggctcg tcgatgcgat gtcgacgcag ccgaagcgtg
    10621 acgaggccgc ggcgctgctc ggtaagctcg cgagcgagat cgcggcgcgc aagaaatagg
    10681 gtctgacgcc gtttcgccgc gcctgatccg gctcgttgat gcgatgtcga cgcagccaaa
    10741 gcgtgacgag gccgcggcgc tgctcggcaa gctcgcgagc gagatcgcgg cgcgcaagaa
    10801 atagggtctg cccggtctgc ccccgtttcg cctcgcggaa cggaaatacg gggtctgacc
    10861 ggtctgaccc cttttcttgc tacggcaggt acgcgagctc gatgcacttg tcggcgagcg
    10921 tgtccgcgtg cccccgcatg ccgggctcga gcgtcacgca cagcttgttg tacccgggca
    10981 gcagcacgcc ctggaacgac agcatgccgt tgttgaacat catgcccatc ggctcgccga
    11041 ggtgaaccat gccgtcgtcg ggaatcacgt aggcgttgac gacgtcggcg ttcgcgccgg
    11101 tcatgctcaa catgaccttg ttcgtcgtga cgaccgtcgg cacgttcacg aaggcgggtc
    11161 cgcgatgcag gctcgggttc gacgacggca gcgaaccgct cgctacgtcg acgcgcacgc
    11221 gaccgggcgc gcccgcgcca ccggcgccag cgcctgtacc accgctcgcg ccaccaccgc
    11281 caggcgtcgc ggcgatcgca cccgagacga tcgtaccggt gtcgctgcgc agcacgacgg
    11341 taccgccgga cccaccgccg ccgccgccgg acgcgcccag gcttagcacc gacgaaccag
    11401 cgccgccggc gccgcctttc gcgtcgatcg taccgacctc gatgttgccg cccgcggtga
    11461 gctcgatggt gccaccgccg ccgccaccgc cgccggagtt cgacagcgca cccttgccgc
    11521 cgccgccgcc gccggcacct tgtgcggcga agctgatgat ctggttatcg ccgtgcgcac
    11581 cgccgcccgc accacccgtg ccggcgtcgc cggtgacgcc gggatttccc gccaccgcaa
    11641 agccgccacc accgccgcca gagtcgccgc ctgcgccaac gccaccgccg tcgctttgca
    11701 agcacggcgc attcgacgcc ggtgcaccgc ccgcgcacgc gccgggtcca gcgccgccgg
    11761 caccgttcgt ggagccattg ccgccgttcg cggtgatgtt gcccatcttg atccgcgata
    11821 ccgagcgaac actcgcgcgc gcggtccccg tgaacgttac gttgccggtg ttcacgtcgg
    11881 agtagagctc ggcgagcgag cctgtcgcga tcgacgtgcc cgccggaaac tgcggcaggt
    11941 tctgtaggct cagcttgttg gtgagcgcca cgccgccgcc cggcccgccg ttctgcatca
    12001 ccgtgatcgt cagcggcgtc atgcctgcgt tcgcgttcgt gtcgatcgcg accgaaactt
    12061 ccacggcgat gtagtcgcca ttcatcgcca ccgtcgtcgc ccccacggtg atgccagtgc
    12121 tcggcgtgat cgcgaccgag agattgtcgc tgacgatgtg gtgacctttg atcacgagca
    12181 ccgccgggcg actgccgaac tggccctggc cttcgaggag aactttcggc gtcacgtcga
    12241 gtagcgtgag catgccgggg ttcgcgtcgg cgagcggcgg cgcatcgatg tccgcatccg
    12301 cagtcatctc ggcgtcgatc atcggcgcgt cggaggcatc gggattcgcc ttgccgaggt
    12361 tgctcgggtt gtagagaagc gagcaaccac tgccgaccac catcggcatc agcattgcca
    12421 cgtactgcct cataaaccga ttatatccgt ggattctcgt cgcccggggg ctcacgccag
    12481 actcacgctc gacaaccagc tcaccgacga ctaccccgaa ttcaccgtcg tctaaccctc
    12541 cagttctagt ttctcgacgg tgatcgaccg aagatgaatc taatgggtgg acatcgaatc
    12601 gtcgtgacgt gcctcttcgc ggcaggctgt ttgccgcgcc ccgagccagc gcaaccgcaa
    12661 caaccgcagc cgcagtatgc gcagccgcaa tacgcgcaac cgcaatacgc gcagccgc
//
LOCUS       DASUFX010000168         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1694182, whole genome shotgun sequence.
ACCESSION   DASUFX010000168 DASUFX010000000
VERSION     DASUFX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694182"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..833)
                     /locus_tag="VFV99_06750"
     CDS             complement(54..833)
                     /locus_tag="VFV99_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059039.1"
                     /translation="MSDNDDDDKVKKLLAEAHDAGALSAKALAALELVDVGAQIQAGL
                     GVTIDDVAASEVVLLTMMPDDSQSIAAANNTDAVRDGHNFVLDALGKSKQSGEVLAHT
                     RYLNGSVLFPYTQLAHAQRMTAANYDPCLGTPLFDQAVVLLGTVIAKAQELAQAGIAV
                     RTVTLIITDGGDYGSTRFKPQDVAAIVADMLGQENHIVAAMGISDGTTDFKKVFRAMG
                     IPDKWILTPGNSSSEIRRAFQVFSQSAVRASQGLQLGGFSN"
     gene            complement(847..1890)
                     /locus_tag="VFV99_06755"
     CDS             complement(847..1890)
                     /locus_tag="VFV99_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEX5059040.1"
                     /translation="MLPIPSFFDAKHAAEWAYRPDAAQLATAANAWRAQHKIKPSAAD
                     ETRSHLLLIDVQKDFCFPDGTLYVAGRSGRGAIDDSRRIAEFVYRNLGALTDVTTTLD
                     THLAYQIFFPSFWLDKADQPLTPFRTITSDEIAAGEVRPNPAMAKWLCGGNYTWLCKQ
                     VLYYCKELERAGKYQLYLWPPHCLLGSDGHALAGVIHEARLFHAFARTSQSHVEVKGG
                     NPLTENYSVLRPEVLSRFDGAALAQRNTQFIQTLLAADAVVIAGQAASHCVKSTIDDL
                     LTEIAAQDPALASKVYLMTDCMSAVTVPDGKGGFAADFTAQADAALQKFADAGMHLVK
                     STDPITSWPGIRL"
     gene            complement(1899..>2551)
                     /locus_tag="VFV99_06760"
     CDS             complement(1899..>2551)
                     /locus_tag="VFV99_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059041.1"
                     /translation="LRYQTIPIADVAIASHRVGRSGAVWLDGGALMRSTRLGPERIGS
                     VLAGQTQAWVGAKLGIGFYRAGGYAVGFVFRPDRGVLDDRVTLPKIRGQLVAAHATVG
                     DDRAWLFLTCAESGRVVTTCIVIGADASVLAAETLPDATWLAGVGGACAAGPHLFVPT
                     DEGVARIEVVQRTVVATRVFAETAPLVSAGDVLALSSSGGGLDVIRRRDAVRMQLT"
BASE COUNT          427 a          827 c          866 g          431 t
ORIGIN      
        1 acatgactac acctcccttc tggcgcaagt aattgatgca tcgcgcgtac cgactaattc
       61 gagaaaccgc cgagctgaag gccttgcgat gcgcgcaccg ctgattgcga gaacacctgg
      121 aacgcgcgcc ggatctccga cgatgaattg cccggcgtca ggatccactt gtcagggatt
      181 cccatcgcgc ggaacacttt cttgaagtca gtcgtaccgt cactgatccc catcgcagcg
      241 acgatgtgat tctcctggcc gagcatgtcg gcaacgatcg ccgcgacatc ctgcggcttg
      301 aagcgcgtcg agccatagtc gccaccgtcc gtgatgatca gcgtcaccgt gcgcaccgcg
      361 atgccggcct gggcgagctc ctgggccttc gcgatcacgg tgccgagcag gacgaccgcc
      421 tggtcgaaca gcggcgtgcc gaggcacggg tcgtagttcg ccgcggtcat tcgctgcgcg
      481 tgcgcgagct gcgtgtacgg gaacagcacg ctgccgttga ggtagcgcgt gtgcgcgagc
      541 acctcgcccg actgtttcga cttcccgagc gcgtcgagca cgaagttgtg gccgtcgcga
      601 acggcatctg tgttgttcgc cgcggcgatc gactgcgagt cgtccggcat catcgtcagc
      661 agtacgacct cgctcgcggc gacgtcgtcg atcgtcacgc cgaggccagc ctggatctgc
      721 gcgcccacgt cgacgagctc gagtgcagcg agtgcctttg cggatagcgc gccggcgtcg
      781 tgcgcttcag caagcagctt cttgaccttg tcatcatcat cgttgtcgct catgcgtggt
      841 cactccttac agccggatgc ccggccagct ggtgattgga tctgtcgact tcacgaggtg
      901 catgcccgcg tccgcgaact tctgcagcgc ggcatcggct tgcgcggtga agtccgccgc
      961 gaagccacct ttgccatcgg gaaccgtgac ggccgacatg cagtcggtca tgagatagac
     1021 cttgctcgcg agcgccggat cttgcgcggc gatctcggtg agcaagtcat cgatcgtgct
     1081 cttcacgcag tggctcgcag cctggcccgc gatcacgacc gcatcggcgg cgagcagcgt
     1141 ctggatgaac tgcgtgttgc gttgcgcgag cgcggctccg tcgaagcgcg atagcacctc
     1201 gggacgcagc accgaatagt tctcggtcag cgggttgccg cctttcacct cgacgtgcga
     1261 ctgcgacgtg cgtgcgaatg cgtggaatag ccgcgcctcg tggatcacgc cggcgagcgc
     1321 gtgaccgtcg gagccgagca agcagtgcgg cggccacaag tacagctgat acttgccggc
     1381 gcgctcgagc tccttgcagt agtagagcac ctgcttgcac agccacgtgt agttgccgcc
     1441 acacagccac ttcgccatcg cgggattcgg gcggacctcg ccggccgcga tctcgtcgga
     1501 ggtgatcgtg cggaatggcg tgagcggctg gtcggctttg tcgagccaga agctcggaaa
     1561 gaagatctga tatgcgaggt gcgtatcgag cgtcgtcgtg acgtcggtca gcgcgccgag
     1621 gttgcggtag acaaactcgg cgatgcgccg cgagtcatcg atcgcaccgc gaccggatcg
     1681 gcccgcgaca taaagcgtgc cgtcgggaaa gcagaagtct ttctgcacgt cgatgagcag
     1741 caggtgtgag cgcgtctcgt cggcggcgga cggtttgatc ttgtgctgcg cgcgccacgc
     1801 attcgcggcg gtcgcgagct gagcagcgtc ggggcggtag gcccactcgg ctgcgtgctt
     1861 ggcgtcgaag aagctgggaa tcggcaacat gtggactcct aggtgagctg catgcgaacg
     1921 gcgtcgcgtc gacgaatgac gtcgaggccg ccgccggaac tggagagcgc gagcacgtcg
     1981 cccgcagata cgagcggcgc ggtctcggcg aacacgcgcg tcgcgacgac ggtgcgctgc
     2041 acgacttcga tgcgtgcgac gccctcgtcg gtgggcacga acaggtgcgg tcccgccgcg
     2101 cacgccccgc cgacaccggc aagccaggtc gcgtcgggca gcgtctcggc ggcgagcacg
     2161 ctcgcgtcgg caccgatcac gatgcacgtg gtgacgaccc ggccggactc ggcgcaggta
     2221 aggaacagcc acgcgcggtc gtcaccgacg gtcgcgtgcg ccgcgacgag ctggccgcgg
     2281 atcttcggca gcgtgacgcg atcgtcaagc acgccgcgat cgggtcgaaa cacgaagccg
     2341 accgcgtagc cgccggcgcg gtagaagccg atgccgagct tcgcgccaac ccacgcctgc
     2401 gtctggccgg cgagcaccga gccgatgcgc tcgggcccga gccgtgtgct gcgcatgagc
     2461 gcgccgccgt cgagccacac ggcaccgctg cggccgacgc gatgcgatgc gatcgcgacg
     2521 tcggcaatcg gaatcgtctg gtagcgcagc c
//
LOCUS       DASUFX010000169         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1000249, whole genome shotgun sequence.
ACCESSION   DASUFX010000169 DASUFX010000000
VERSION     DASUFX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000249"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFV99_06765"
     CDS             <1..890
                     /locus_tag="VFV99_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059042.1"
                     /translation="ADCNQLDKEQQVPLTANLLPTGTPIGGLQRPLKELADDPVAALC
                     GHKDWDDIDNFVKSIQPVKARKASDEQSVQRGRQLFVDGGCAKCHGGAGWTVSRRFFA
                     PSSATNTSLAQSAFTIPTFFPATWMYNADASGTKRTLVSAQPAIAAADPTGPEEPAPI
                     TIAQTACTLRNVGTFGVPGDTTKTDALEKRPFQGSLVRAEGRGGYNVPSLYGLALGAP
                     YLHHGQSPSLTDLFTNPAWQFHTNAGNANFSLTLAGAGKVDDLAAFLLSIDATTPEIA
                     VPTDAASGLSFDACPLQFP"
     gene            complement(1014..1646)
                     /locus_tag="VFV99_06770"
     CDS             complement(1014..1646)
                     /locus_tag="VFV99_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059043.1"
                     /translation="MKPNKTLVMLAFLLAPGLAAAQGYYGGSGPGYYNNPAPNPGGFH
                     QRAGRLTWGGSLGLGGMHDDGSNVTSCNNCDYNPLAVMLSGHIGGMLSNRFALLGEFQ
                     VNAQTVHSDFYDGDTILSQNVLMIAGQYWLTPQLWIKGGIGFAHLQADDSYFAYDLAD
                     GGAIMGGIGYELMSARNFSVDLQGRLIQGSYDSFNDSVTSGTVGVGINFY"
     gene            complement(1777..3981)
                     /locus_tag="VFV99_06775"
     CDS             complement(1777..3981)
                     /locus_tag="VFV99_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5059044.1"
                     /translation="MASAVLTQALATPMHASEHTQIVEAFLLIEQPRQRLGHWTKALA
                     ALDTPDAPPTLAVIAYYDPTSDQALIDLQYERAVANMKFVVEVAILAEVGMVQPAELS
                     EGERARFLADRLTRCTIRVTTQSTAASALTELVRRIKDGRMNKVSNPPPPPPARTVQP
                     RGDTQDPVMLVNAKGTRDDLERLDANADFVDPLAGIDDALGDGPEPPPPPRRDNTSHE
                     RPMTKADASHYFKSAERNRTVEMPFEMSQRLIVESSQMMKLSDLKPPGYREPHEFPLP
                     PPAETPAPEVMRAKSSERTPPPIPIRRTEKITVRAKSPNLAELKSPPHRPSGPIADLK
                     PPTHRPSGPIADLKQPTQRPSGPIADLKPPTQRPSGPIADLRQPTQPPQKMPPTMGRH
                     VTPPPRARTDAQRTDPYLPTGGPALPNVIYARYLRSGRWVPVRIGALSLKGAALLAGA
                     LPRANDRVDVAFAFGSYRALVRGTVLKVSTMREATATGAATFSIAFDLDDGSRRQLTN
                     LLTAARDAKITIKPPPPRATRRFPVEWQVALGTIRGAVKATALDVSTHGMFVQPAAAL
                     EVGATLTFSVMLDDGTAPIAGRAKVVRQIKEAEAKECGLLPGFGLLLVSMSEADRMRW
                     LGFLARIERRADKRVLIGADPARLAELQAGLASLGYAVTGGTDPGALVQLANSDGRQA
                     DAVLIDAGWLQNEASASLMENLFSARNVPCVTMQGEVRRARQAIDKLLEVVV"
     gene            4161..>5173
                     /locus_tag="VFV99_06780"
     CDS             4161..>5173
                     /locus_tag="VFV99_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5059045.1"
                     /translation="MKDDREPRGSGYRLPLLPLRDIIVFPHMVVPLFVGRDKSLLALE
                     DAMAGDKQLLLAAQRRAKTDDPSEEDIFAIGTVGHIIQLLRFPKGPVKVLVEGRARAR
                     IRGFSQIDPFFVCDVDDVEEPDEDTTEVQALMRSVQDVFEDYVKLNTRIPPEMLVSVR
                     TIDEPGRLADTIVAHVALKLKDKQELLETPSPSKRLERLGELMQGEIEIRQVEKKLRT
                     RVKKQMEKQQKEYYLNEQMQAIQKELGDRDEFKNELNELEQKIKQKKMSKEAKERCIK
                     ELKKLKMMSPISAEATVVRNYIDWVIALPWDDKTEDRHDINEAEKILEAEHYGLKKVK
                     ERIL"
BASE COUNT          953 a         1670 c         1680 g          870 t
ORIGIN      
        1 cggccgactg caaccagctc gacaaggagc agcaggtgcc gctcaccgcg aacctgctgc
       61 cgacgggcac gccgatcggc ggtctgcagc ggccgctcaa ggagctcgcc gacgatccag
      121 tcgccgcgct gtgcggtcac aaggattggg acgacatcga caacttcgtg aaatcgatcc
      181 aaccagtgaa ggcgcgcaag gcgagcgacg agcaatcggt gcagcgcggt cgccagctat
      241 ttgtcgacgg cggttgcgcg aagtgccacg gcggcgcggg ctggacagtg tcgcgtcgct
      301 tcttcgcgcc gtcgagcgcg acaaacactt cgctcgcgca gagcgcgttc acgatcccga
      361 cgttcttccc ggcaacctgg atgtacaacg ccgatgcgag cggcacgaag cgcacgctgg
      421 tgtcggcgca gccggcgatc gccgcggcag atccgaccgg tcccgaggag ccggcaccga
      481 tcacgatcgc gcagaccgca tgcacgctac gtaacgtcgg cacgttcggc gtgcccggcg
      541 acacgacgaa gacggacgcg ctcgagaagc gtccgttcca gggctcgctc gtgcgcgcgg
      601 aaggtcgtgg tggctacaac gtgccgtcgc tctacggcct cgcgctcggt gcgccgtacc
      661 tgcatcacgg ccagtcgccg tcgctgacgg atctgttcac gaacccggcg tggcagttcc
      721 acacgaacgc cggcaacgcg aacttcagtc tgacgctcgc gggcgcaggc aaggtcgacg
      781 atctcgcggc gttcctgctc tcgatcgatg cgacgacgcc cgagatcgcg gtgccgaccg
      841 acgcggcgag tggcctgtcg ttcgacgcgt gtccgctcca gttcccgtag caggcgaaag
      901 aggtcacctg ccgcggggcg cccgcgcatc acttctggtg cgcgggcgtt ttttcttgcc
      961 cgaaaacaac aacaccccca gtgcgggggc gctgccgtca ggaaccacga acgctagtag
     1021 aagttgatgc cgacgccgac ggtgcccgac gtgacgctgt cgttgaagct gtcgtacgag
     1081 ccctggatca ggcgaccctg caggtcgacc gagaagttac gcgcgctcat cagctcgtag
     1141 ccgatgccgc ccatgatcgc gccgccatcg gcgaggtcgt acgcgaagta cgagtcatcg
     1201 gcctgcaggt gcgcgaagcc gatgccgccc ttgatccaca gctgcggcgt gagccagtac
     1261 tggccggcga tcatcagcac gttctgcgac aggatcgtgt cgccgtcata gaagtccgag
     1321 tgaacggtct gcgcgttgac ctggaactcg ccgagcagcg cgaaccggtt gctcagcatg
     1381 ccgccgatgt gacccgacag catgaccgcg agcgggttgt agtcgcagtt gttgcagctg
     1441 gtgacgttgc tgccgtcgtc gtgcatgccg ccgagaccga ggctaccgcc ccaggtcagt
     1501 cggccggcgc gctggtggaa gccgcccggg ttgggcgccg ggttgttgta gtagcccggt
     1561 ccgctgccac cgtagtagcc ctgcgcggcc gcgaggcccg gggcgagcag gaaggcgagc
     1621 atgacgagtg tcttgttagg cttcatggct cccctatcat cgcaagtctg gggccagcac
     1681 gccaacgccc aaaccctcag tggtccacgt agttacaagg ttggaccgga gaacaatcgt
     1741 tcacagctgg cgcgattcaa caacgcgtgt gtgagatcac acgacaactt caagcagctt
     1801 gtcgatcgcc tggcgtgcac gtcgcacctc accctgcatc gtcacgcacg gcacgttgcg
     1861 cgcgctgaat aggttttcca tcagcgatgc actcgcttca ttttgcagcc agccggcatc
     1921 gatcaacacc gcgtctgcct gacggccatc cgagttcgcg agctgcacga gcgcgcccgg
     1981 atcggtgccg ccggtgaccg cgtagccgag gctcgcaagt ccagcctgca gctcggcgag
     2041 ccgcgccgga tccgcaccga tcagcacgcg cttgtccgcg cgccgctcga tgcgcgcgag
     2101 gaagccgagc cagcgcatgc gatccgcttc cgacatgctg acgagcaaga ggccgaagcc
     2161 gggcaagagg ccgcactcct tcgcctcggc ctctttgatc tgccgcacga ccttcgcgcg
     2221 tcctgcgatc ggtgcagtgc catcatcgag catcaccgag aacgtgagcg tcgccccaac
     2281 ttcgagcgca gcagccggct gcacgaacat gccgtgcgtc gagacatcga gcgccgtcgc
     2341 tttcaccgca ccgcgaatcg tgccgagcgc gacttgccac tcgaccggaa accgacgcgt
     2401 cgcacgcggt ggcggcggct tgatcgtgat cttcgcgtcg cgcgcagcgg tgagcagatt
     2461 ggtcagctgg cggcgcgaac cgtcatcgag atcgaacgcg atagagaacg tcgccgcgcc
     2521 ggtcgccgtt gcctcgcgca tcgtcgacac cttgagcacg gtgccgcgca cgagcgcacg
     2581 atacgaaccg aacgcgaacg cgacatcgac gcgatcgttc gcacgcggca acgcgcccgc
     2641 cagcagcgcc gcgcccttca agctgagcgc accgatgcgc accggcaccc agcgcccgct
     2701 gcgcagataa cgcgcgtaga tcacgttcgg cagcgccggg ccgccggtcg gcagatacgg
     2761 atccgtgcgc tgagcatcgg tgcgcgcgcg cggcggcggc gtcacgtgac ggcccatcgt
     2821 cggcggcatc ttctgcggag gctgcgtcgg ctgtctcaga tcggcgatcg gcccggacgg
     2881 acgctgcgtc ggcggcttca gatccgcgat cggccccgac ggacgctgcg tcggctgttt
     2941 cagatcggcg atcggccccg acggacggtg cgtcggcggc ttcagatccg cgatcggccc
     3001 cgacggtcgg tgcggcggcg acttcagctc ggcgagattc ggcgacttcg cgcgcacggt
     3061 gatcttctcg gtgcgacgaa tcgggatcgg cggcggcgtt ctctcgctcg acttcgcgcg
     3121 catgacctct ggcgcggggg tctcggccgg cggcggcaac gggaactcat ggggctcgcg
     3181 atagccgggc ggcttcaagt cgctgagctt catcatctgc gacgactcga cgatcagtcg
     3241 ttgcgacatc tcgaacggca tctcgacggt gcgattgcgc tcggcgctct tgaagtagtg
     3301 cgacgcgtcc gccttcgtca tcggtcgctc atgagaagtg ttgtcacgac gcggtggcgg
     3361 cggcggctcc gggccatccc cgagtgcatc gtcgataccg gcgagaggat cgacgaagtc
     3421 ggcgtttgca tcgaggcgct cgagatcatc gcgcgtgccc ttcgcgttga cgagcatcac
     3481 tggatcctgt gtgtcgccgc gcggctgcac ggtgcgtgcg ggtggtggtg gcggcggatt
     3541 cgagactttg ttcatacgtc cgtctttgat ccttcgtacg agctcggtca gcgcggatgc
     3601 ggcggtggac tgcgtggtga cgcgaatcgt gcagcgcgtg agtcgatcgg cgaggaaccg
     3661 cgcgcgctcg ccttcggaca gctctgccgg ctgcaccatg ccgacctcgg caaggatcgc
     3721 gacctcgacg acgaacttca tgttcgccac cgcgcgctcg tactgcagat cgatcagcgc
     3781 ctgatcgctc gtcgggtcgt agtacgcgat cactgcgagc gtcggcggtg cgtccggcgt
     3841 atcgagcgca gcgagtgctt tggtccagtg tccgaggcgt tgccgaggct gctcgatcag
     3901 aagaaatgct tcgacgatct gcgtgtgctc gctcgcgtgc atgggtgtag ccagtgcctg
     3961 cgtgagcacc gcagatgcca cggacaccta gtgaaacgcg ccttacagct gaatagagat
     4021 agagcgggat tccttacgat cgctgtgcac cgatcgcaca ggcatttcgt ggcatcagaa
     4081 ccacgactat cgtggcaatt ctaccactca gactttggtt ttcttgccca agtccccgtc
     4141 caccctaaag tatcgtggat atgaaagacg accgcgagcc acggggtagt ggctaccgtc
     4201 tgccactgct accgctccgc gacatcatcg tgttcccgca catggtcgtg cccttgttcg
     4261 tgggtcgcga caagtcgctg ctcgcgctcg aagacgcgat ggcgggagac aaacagctgc
     4321 tgctcgcagc acagcgtcgc gcgaagaccg acgatccatc cgaagaggac atcttcgcga
     4381 tcggcacggt cggtcacatc atccagctgc ttcgcttccc gaaaggaccg gtgaaggttc
     4441 tcgtcgaggg tcgcgcgcgt gctcgcattc gcggcttctc gcagatcgat ccgttcttcg
     4501 tctgcgatgt cgacgatgtc gaagagccgg acgaggacac caccgaagtc caagcgctga
     4561 tgcgctcggt gcaagacgtc ttcgaagact acgtgaagct caacacgcgc atcccgccgg
     4621 agatgctcgt cagcgtgcgc acgatcgacg agcccggtcg gctcgccgac acgatcgtcg
     4681 cgcacgtggc gctcaagctc aaagacaagc aagagctcct cgaaacgccg tcaccatcga
     4741 agcgcctcga gcgtctcggc gagctgatgc aaggcgagat cgagattcgt caggtcgaga
     4801 agaagctgcg cactcgcgtc aagaagcaga tggagaagca gcaaaaagag tactacctga
     4861 acgagcagat gcaggccatc cagaaggaac tgggtgaccg cgacgagttc aagaacgagc
     4921 tcaacgagct cgagcagaag atcaagcaga agaagatgtc caaggaggcc aaggagcgct
     4981 gcatcaagga gctcaagaag ctcaagatga tgtcgccgat aagcgccgag gccacggtgg
     5041 tgcgcaacta catcgactgg gtgatcgcgc tgccctggga cgacaagacc gaggatcgcc
     5101 acgacatcaa cgaagccgaa aagatcctcg aggccgagca ttacggtctc aagaaagtca
     5161 aagagcgcat tct
//
LOCUS       DASUFX010000170         7857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1138497, whole genome shotgun sequence.
ACCESSION   DASUFX010000170 DASUFX010000000
VERSION     DASUFX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138497"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1091)
                     /locus_tag="VFV99_06785"
     CDS             complement(111..1091)
                     /locus_tag="VFV99_06785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5059046.1"
                     /translation="MSYTIDDRVMPVVTREELESSLMELARDVRDPHEGILGPRSVAW
                     KLGGDLAVFLGGGRAALLQLAHPMVAFAVDHHSRTRADVVGRFQRTFRNVFAMVFGEL
                     DEAFTAARRVHSIHTRIHGEIPHQIGGWRAGTPYYANDVDALRWVHATLVDTTIAVRE
                     RLDGALPSALKDTYIVEMNRFGALFGIARDRLPDSWAAHESYMQRMLGSDLLAVAPCA
                     KEMATFLMGRTEGHAQPPLGRFAEALTATMLPKHLARDFGLQRTTLSTTGVQLALSAF
                     APIYRRVPTRYVAIPARSMATRRLRGQGPSRFASWTERQLFGLSRQVTGS"
     gene            1226..2713
                     /locus_tag="VFV99_06790"
     CDS             1226..2713
                     /locus_tag="VFV99_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059047.1"
                     /translation="MVPTASSAVVQPRGEPASFESASDDALPPGAKAGPWVVERELGR
                     GGMGAVYGVVHEEIGKRAALKVMHSRLVSGSSMERILLEAKVVNAVGHPNIVDIFETG
                     TLDDGRPYIVMERLEGVPLSFRADEGKILPDQVIGILLQVCDALIAAHAANIVHRDLK
                     LDNVFLIDNVDDPSSPRVKLLDWGIAKVISNDVRHTIEGQLVGTPQYLAPEQARGAAV
                     SPATDVYSLGVMAYELFLEQLPFEAETSAEIMAMHLRCTPPPPSELWPDIPATLEHLL
                     LAMLEKNADDRPSMLTVAHTLERVRADLDARRQPVAETTAARTPTVPPLRRSSRHDVG
                     HRSSNQLAQAFNGFSPTEPAAWPETRRHWHVAIGALAVAASAAMFLLTRNTERAAAAA
                     PKREAVVEQVTATTEPAPPSPVAPDAPDIAPPSREPSPAVIPAAFPIDPYADAADTEA
                     TAIKPAVLRSSAASKRSHSHTRKQPVKPRRSPQPLDPDGTLDPYL"
     gene            2710..3492
                     /locus_tag="VFV99_06795"
     CDS             2710..3492
                     /locus_tag="VFV99_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059048.1"
                     /translation="MSVRAITLAAVLAAAPIAHADNALRPERIPNKARELAEKGRAYH
                     NSGDYSAAIAAFKEAYVLAPSPGLLFNIAQAYRLAGDCDESAWMYRRFLDTNPVGEHR
                     SLAETHLASVEKCGRGGLRVTAPTPKQDALAVPEPATSVVTDADPDRRSRTYKRVGLG
                     TAIGGGALLAGAAIFAFDSYDASKTVAETYQHGGKWDDIKDVDARGKRSATIATVMGV
                     GGGLAVVSGAVLFAVGRHYEQAQRVAVTPTAHGAQVSLSWGF"
     gene            3501..4016
                     /locus_tag="VFV99_06800"
     CDS             3501..4016
                     /locus_tag="VFV99_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059049.1"
                     /translation="MASLVAIAGGCYSPELRDCTVTCSAAADCADGQVCGADHYCAAP
                     GIAGQCSTLPADAGLTIRDGGTDARTIVDARVDAPDATVWAAIDISIDGQGRVIVQNI
                     ASCEKAPPQNGSCTFYVPAGLAVTVHAQPYFGWHFDKWTMGACDHVVGMTCTFQANGT
                     SPVGAKFRKDD"
     gene            4016..4456
                     /locus_tag="VFV99_06805"
     CDS             4016..4456
                     /locus_tag="VFV99_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059050.1"
                     /translation="MKTIAALSLALAACYEPDAIDCTVECTAPRDCADGQVCGSDGFC
                     AAPDIAGLCNANNHDDPQLVSLTIEIEGNGKVSVEKVGMCDSESPSAGNCTFSVTPGV
                     AQQLKAVENKERTFVSWTATCTGSSATCALTPVMALTRVGARFE"
     gene            4601..5737
                     /locus_tag="VFV99_06810"
     CDS             4601..5737
                     /locus_tag="VFV99_06810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059051.1"
                     /translation="MAAAVANPDAVLQRASDPANALTRRRGRPDDGLTAGSRVSSWKV
                     DSELGRGGMSSVHAVVHTKFGKRAAIKIAHRSVLGDGGLSAQTFWREARIVHTVEHPA
                     VIDVFATGSHDGRPYLVMEKLIGEPLGRRVDHGPPMPRREAIQILLELCDVLRTAHAA
                     GLVHRDLKLDNVFLCDTLFAGGRRVKLLDWGVAYVEGEPDPFRGQIAGTLTYVAPEQI
                     RGDGLTGAADIYSLAVLAYHLLCRRPPFAGSDLALLNMHLRAEPPPPRFAWPDVPPAL
                     DDLLVRMLAKQPGDRPSLDEVEDTLQAAFAVVDQPPRLAEGSNPNIRLPAEEPWYLRW
                     LMVREGDVQSDVLGRPIVVPPFRPAWMAIGLTISALATLISALT"
     gene            complement(5734..6381)
                     /locus_tag="VFV99_06815"
     CDS             complement(5734..6381)
                     /locus_tag="VFV99_06815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX5059052.1"
                     /translation="MADNDSREGKRYTTPAILDYVNRVHAGHDTALAQAFAVPEGIPA
                     IQVGPSEGKLLYLLLKLANAKKVVEVGTLVGYSAIHMARALPADGHLYSIEYEAKHAE
                     VARKNIERAGLADRITVHVGAGLDVLPTLEKHGPFDAVFIDADKQNYDKYGQWATKHL
                     RKGGLVLGDNSFLFGELLDDSDRGRAMRAFHEHVAQTCDSVNMPTPDGLVVGIKR"
     gene            complement(6397..7584)
                     /locus_tag="VFV99_06820"
     CDS             complement(6397..7584)
                     /locus_tag="VFV99_06820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059053.1"
                     /translation="MASSTNAESAKLGVRVVPSITQVDAAAWDALDHGPSPFLRHGFL
                     RALEESGSIGGTGRRRSGWTPAYLLAERAGQLVGAVAAFIKDHSYGEYIFDWGWASAA
                     QRAGIRYYPKLVIAAPATPATGRRILLAPGAPGEVTAVLLAAVRAIADDTKCSSIHWL
                     FCTAEEQATLARFGFFPRMTLQFHWKNRGYGSFDEFLAQLKSRKRKQLRKERERARAA
                     IDQLAWVTGSEISEAQLDDLDRFYRITTANHGGRDYLREGFFHLLAAHLPDAMRVVEV
                     TKSGRRIAGALFLETDVALYGRYWGADTHVDLLHFETAYYAGIERAIERRIPLFEAGA
                     QGEHKLLRGFEPSTTYSAHWIRHEGLAAAVEDYTTREAMAMATELAEMAKFGPYRSDN
                     DDD"
     gene            7596..>7857
                     /locus_tag="VFV99_06825"
     CDS             7596..>7857
                     /locus_tag="VFV99_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5059054.1"
                     /translation="MALDPQIKSKIDQLVAQDPVVLFMKGTRSFPQCGFSASVVNILN
                     TLIPKYATVNVLSDPDVRGGMKEYSDWPTFPQLFIKGEFVGGA"
BASE COUNT         1330 a         2611 c         2614 g         1302 t
ORIGIN      
        1 gttcgtgctt tgcgtgacgt ctgcatcgcg cggcggcaag ggagcctccc gctttcggca
       61 aaagcacttt cgtgtcgtcg cgcgatgcgt ctccggacag cgtttctggt tcagctgccg
      121 gtgacttgac ggctgagccc gaacagctgg cgctccgtcc acgacgcgaa cctgctcggt
      181 ccctgcccgc gcagccgccg ggtcgccatc gagcgcgccg ggatcgcgac gtagcgtgtc
      241 ggcacccgcc gatagatcgg cgcgaacgcc gacagtgcga gctgcacgcc ggtggtcgag
      301 agcgtcgtgc gctgaagacc gaagtcgcgc gcgaggtgct tcggcaacat cgtcgcggtc
      361 agcgcttcgg cgaagcgacc gagcggcggt tgcgcgtgcc cttcggtgcg ccccatcaag
      421 aacgtcgcca tctccttcgc gcacggcgcg acggcgagta gatcagagcc gagcattcgc
      481 tgcatgtacg actcgtgcgc agcccagctg tccggaagcc gatcgcgagc gataccgaac
      541 agcgctccga agcgattcat ctcgacgatg taggtgtcct tgagcgcgct cgggagcgca
      601 ccatcgaggc gctcgcgcac ggcaatcgtt gtatcgacga gcgtcgcgtg aacccaacga
      661 agcgcgtcta cgtcgttcgc gtagtaagga gttcccgccc gccagccacc gatctgatgc
      721 ggaatctcac cgtgaatcct cgtatgaatc gagtgaacgc ggcgcgccgc ggtgaatgct
      781 tcgtcgagct cgccgaacac catcgcgaac acgttccgga acgtgcgctg gaagcgacca
      841 acgacatccg cgcgcgtgcg cgaatggtga tcgaccgcaa acgctaccat cggatgtgcg
      901 agctggagca gcgccgcgcg tccgccaccg aggaagactg cgagatcgcc accgagcttc
      961 cacgcgacgg agcgcggacc gaggatccct tcgtgcggat ctcgaacatc gcgcgcgagc
     1021 tccatcaatg aagactccag ctcttcgcgc gtcacgacgg gcatgacgcg atcgtcgatg
     1081 gtgtaggaca tcgcggtcaa gcgcgaagcg tgcgcgcgtg ccacaacggg ccgagtcgct
     1141 acgtgaggtg tgcgcgaagt gctggtccgc ggcgctctga gaaccggcat acacgctgca
     1201 cagaaggcga tgcggtatgc agaccatggt gcccacggca agcagcgcgg tggtccagcc
     1261 tagaggtgaa cctgcgtctt tcgagtccgc gtcggatgat gcgcttccgc cgggcgccaa
     1321 agcaggacca tgggttgtcg agcgtgagct cggtcgtggc ggtatgggcg cggtgtacgg
     1381 cgtggtgcac gaagagatcg gtaagcgagc cgcgctcaag gtgatgcaca gccgactggt
     1441 gagcgggagc agcatggagc ggatcctgct cgaggcgaag gtcgtcaacg cggtcggcca
     1501 tccgaacatc gtggacatct tcgagaccgg cacgctcgat gacggccgcc cctacatcgt
     1561 gatggaacgg ctcgaaggcg tgccgctgtc gttccgcgcc gacgaaggca agattctccc
     1621 tgatcaggtc atcggcatct tgctgcaagt ctgcgacgcg ctgatcgccg cgcacgccgc
     1681 gaatatcgtc catcgcgacc tcaagctcga caacgtgttt ctgatcgaca acgtcgacga
     1741 cccgagctcg ccgcgcgtga agctcctcga ctggggtatc gcgaaggtga tctcgaacga
     1801 cgtgcgccac acgatcgagg gtcagctcgt cggcacaccg cagtatctcg cgcccgagca
     1861 ggcgcgtggc gccgccgtct cgcccgcaac cgacgtctac tcgctcggcg tgatggcgta
     1921 cgagctgttc ctcgaacaac tgccgttcga agccgagacc agcgccgaga tcatggcgat
     1981 gcacctgcgc tgcacgccac ctccgccgag cgagctatgg cccgacattc cggcgaccct
     2041 cgagcatctg ttgctcgcga tgctcgagaa gaatgccgac gaccggccga gcatgctcac
     2101 ggtcgcgcac acgctcgagc gagtgcgtgc ggatctcgac gcgcgccggc aaccggtggc
     2161 cgagaccaca gcggcccgca cacccaccgt gccgccgctc cgtcgcagct cgcggcacga
     2221 cgtcggtcat cgcagctcga accagctcgc acaagcgttc aacggattct cgccgaccga
     2281 gccggccgcg tggccggaga cgcgccggca ctggcatgtc gcgatcggtg ctcttgccgt
     2341 tgccgcgagc gcggcgatgt tcctgctcac gcgtaacacc gagcgtgcgg ctgccgctgc
     2401 accgaagcgc gaggcagttg tcgagcaggt gaccgcgacg acggaaccgg cgccgccatc
     2461 gcccgtcgcg cccgacgcgc ccgacatcgc accgccgagc cgcgagccga gtcccgcggt
     2521 cattcccgcc gcatttccga tcgatcccta cgcggatgcc gcggacaccg aggcgaccgc
     2581 gatcaagccc gccgtgctgc gtagcagtgc tgcatccaag cgaagccaca gccacacgcg
     2641 gaagcaaccg gtcaagccgc gccggagccc gcagcctctc gatcctgacg gcacgctcga
     2701 tccgtacctg tgagcgtccg tgcgatcaca ctcgccgcgg tcctcgcagc ggcgccgatc
     2761 gcgcatgctg acaacgcgct gcggcctgaa cggatcccga acaaggcgcg cgagctcgcc
     2821 gagaagggcc gcgcgtatca caacagcggc gactacagcg ccgccatcgc cgcgttcaag
     2881 gaagcgtacg tgctcgcgcc gagcccgggc ctgttgttca acatcgcgca ggcgtatcga
     2941 ctcgccggtg actgcgacga atcggcgtgg atgtatcgcc gcttcctcga cacgaatccc
     3001 gtcggcgagc atcgctcgct cgccgaaacg cacctcgcat ccgtcgaaaa gtgcggccgc
     3061 ggcggcttgc gcgtgaccgc accgacaccg aagcaggacg ccctggccgt gcccgagccc
     3121 gccacgagtg tcgtgacgga cgctgatccc gaccggcgca gccgcacgta caagcgcgtc
     3181 ggtctcggca ccgccatcgg tggcggtgcg ctgcttgccg gtgcggcgat cttcgcgttc
     3241 gattcatacg acgcgtcgaa gacggtcgcc gagacgtacc agcacggcgg caagtgggac
     3301 gacatcaagg acgtcgatgc gcgtggcaag cgcagcgcga cgatcgcgac agtgatgggt
     3361 gtaggcggcg ggctcgcggt ggtgtcgggc gcggtgttgt ttgccgtcgg acggcactac
     3421 gagcaagcgc agcgcgtcgc ggtgacgcca actgcgcacg gggcccaggt cagcctctca
     3481 tggggatttt gagagccggc gtggcttcgc tcgtcgccat cgccggaggt tgttattcgc
     3541 cggagctccg cgattgcacc gtcacatgca gcgctgcggc cgattgcgca gacggccagg
     3601 tctgcggcgc agatcattac tgcgctgcgc ccgggatcgc cggccagtgc tcgacgttgc
     3661 cagcggatgc cggcctaacg attcgcgatg gcggcacaga tgcgcgaacg atcgtcgatg
     3721 cgagagtcga cgcgcccgac gcgaccgtgt gggccgcgat cgacatctcg atcgacggcc
     3781 agggacgagt catcgtgcag aacatcgcat cgtgcgagaa ggcgcctccg cagaatggct
     3841 cgtgcacgtt ctacgtgccc gccggtctcg cggtcaccgt tcatgcacaa ccgtacttcg
     3901 ggtggcattt cgacaagtgg acgatgggcg cgtgtgacca cgtcgtgggc atgacgtgca
     3961 cgttccaagc gaacggcacc tcgcccgtcg gcgctaaatt tcggaaagac gattgatgaa
     4021 gacgatcgcc gcgctctcgc tggccctcgc cgcctgctac gaaccggacg ccatcgactg
     4081 caccgtcgag tgtacggcgc cgcgtgattg cgccgacggt caggtgtgcg gcagcgacgg
     4141 attctgtgcc gcgccggaca tcgcaggcct gtgcaacgcg aataaccacg acgacccgca
     4201 gttggtgtcg ctgacgatcg agatcgaggg caacggcaag gtgagtgtcg agaaggtcgg
     4261 catgtgcgat agcgaaagcc cgtcggcggg gaactgcaca ttttccgtta cacccggtgt
     4321 ggcgcaacag ctcaaagcgg tcgagaacaa ggaacgtacg ttcgtgtcat ggacggcgac
     4381 gtgcactggc agcagtgcga cgtgcgcact cactccagtg atggcgctga cacgtgtggg
     4441 agcgcggttc gaatgactcc gaaccgcgag ctgcgttcgc acgtgcgtgg ccgccacgtt
     4501 cgagcgtcgt cgcgcattgc gtcttgcgag gtatccccat gtgccgcggt gttcgggctc
     4561 ctgtgcgctg gtccactccg tgcacaggct ccgaacatct atggcagcag cggttgcgaa
     4621 ccccgacgca gttctgcagc gggcgagtga tccagcgaac gcgctcacgc ggcggcgcgg
     4681 caggcccgac gatggcctca ctgccggctc gcgtgtgagc tcctggaagg tcgacagcga
     4741 gctcgggcgt ggtgggatgt ccagcgtcca cgccgtcgtc cacacgaagt tcggcaagcg
     4801 cgcggcgatc aagatcgccc atcgttcggt acttggagat ggtggcctgt cggcacagac
     4861 gttctggcgc gaggcacgta tcgtccatac tgtcgagcat cctgccgtca tcgacgtatt
     4921 cgcgaccggc tcccacgacg gccgaccata cctcgtgatg gagaagctca tcggcgaacc
     4981 gctcggccgc cgcgtcgacc acggcccgcc gatgccacgt cgcgaggcaa tccagatctt
     5041 gctcgagctc tgcgacgtgc tgcgcacagc gcacgctgcc ggcctcgtcc accgcgatct
     5101 gaaactcgac aacgtgttct tgtgcgacac gctgtttgcc ggtgggcgtc gcgtgaagct
     5161 gctcgactgg ggcgtcgcat atgtcgaagg cgaaccggat ccgttccgcg gccagattgc
     5221 cggcacgctg acgtacgtcg cgcccgagca gatccgcggg gacggcctga ccggcgcggc
     5281 cgacatctac tcgctcgccg tgctcgcgta tcacttgctg tgccgccgcc cgccattcgc
     5341 cggcagtgac ctcgcgctac tcaacatgca cctgcgtgcc gagccgccgc cgccgcgctt
     5401 cgcgtggccc gacgtgccgc ccgcgctcga cgacctgctc gtgcgcatgc tcgcgaagca
     5461 gcccggcgat cgaccgtcgc tcgacgaggt cgaggacacg ctgcaggctg cgttcgcagt
     5521 cgtcgatcag cccccgcggc tcgcagaagg ttcgaatccg aacatcaggc tgcccgctga
     5581 agaaccgtgg tacctgcgct ggctgatggt gcgcgagggc gacgtgcaga gcgacgtgct
     5641 cggcaggccg atcgtggtgc caccgttccg acccgcgtgg atggcaatcg ggctcacgat
     5701 cagcgcgctc gcgacgctga ttagcgcgct gacctagcgc ttgatcccga cgacgagacc
     5761 gtcgggcgtc ggcatgttca ccgagtcgca cgtctgcgcg acgtgctcgt ggaacgcacg
     5821 catcgcgcgg ccgcgatcgc tgtcgtcgag cagctcgccg aacaagaacg agttgtcgcc
     5881 gagcacgagg ccgcctttgc gcaggtgctt cgtcgcccat tgaccgtact tgtcgtagtt
     5941 ctgcttgtcg gcgtcgatga acaccgcgtc gaacggcccg tgcttctcga gggtcggcag
     6001 cacgtcgagg ccggcaccga cgtgcaccgt gatgcgatcg gcgaggcccg cgcgctcgat
     6061 attcttgcgc gcgacctccg catgcttcgc ttcgtactcg atcgaataga ggtgaccgtc
     6121 tgcgggcagc gcgcgcgcca tgtggatcgc cgagtagccg acgagcgtgc cgacttcgac
     6181 gaccttcttc gcgttcgcga gcttgaggag caggtagagc agcttgcctt cgctcgggcc
     6241 gacctggatc gccggaatgc cttccggtac cgcgaacgcc tgcgcgagcg cggtgtcgtg
     6301 gcccgcgtgc actctgttga cgtagtcgag gatcgcggga gtcgtgtagc gcttgccttc
     6361 gcgagagtcg ttgtcggcca tggcgtgatc taggcgctaa tcgtcgtcgt tgtcactgcg
     6421 gtacgggccg aacttcgcca tctcggcgag ctcggtcgcc atcgccattg cctcgcgggt
     6481 cgtgtagtcc tcgaccgcgg ccgcgaggcc ttcgtgtcga atccagtgcg ccgagtacgt
     6541 agtgctcggc tcgaaccctc gcaacagctt gtgctcgccc tgcgcgccgg cctcgaacag
     6601 cgggatgcgg cgctcgatgg cgcgctcgat accggcgtag tacgcggtct cgaagtgcag
     6661 caagtcgacg tgcgtgtcgg cgccccagta gcggccgtag agcgcgacat cggtctcgag
     6721 aaacagcgcg ccggcgatgc ggcgaccgct cttcgtcacc tcgacgacgc gcatcgcgtc
     6781 gggcagatga gcggcgagca ggtgaaagaa tccttcgcgc agataatcgc ggccaccgtg
     6841 gtttgccgtc gtgatgcgat agaagcgatc gaggtcgtcg agctgtgctt cgctgatctc
     6901 gctgccggta acccacgcga gctgatcgat cgctgcgcgc gcgcgttcgc gttccttgcg
     6961 gagctgctta cgcttgcggc tcttgagctg cgcgaggaac tcgtcgaacg agccgtagcc
     7021 gcggttcttc cagtgaaact gcagcgtcat gcgcgggaag aagccgaacc gcgcgagggt
     7081 cgcttgttct tcggcggtgc agaacagcca gtgaatcgac gagcacttcg tgtcatcggc
     7141 gatcgcgcgc accgcggcga gcagcacggc ggtgacctcg ccaggcgcac cgggtgcgag
     7201 caggatgcgg cggccggtcg cgggcgtcgc cggcgcggcg atcacgagct tggggtagta
     7261 gcggatgccg gcgcgctgcg cggcgctcgc ccagccccag tcgaagatgt actcgccgta
     7321 gctgtggtcc ttgatgaatg cggcgaccgc gccgacgagc tgccctgccc gttcggcgag
     7381 cagatacgcg ggagtccagc cgctgcgccg ccggccggtg ccgccgatcg accccgattc
     7441 ttcgagtgcg cgcagaaagc catgacggag aaatggcgag gggccgtggt cgagggcgtc
     7501 ccacgccgcg gcatcgacct gcgtgatgct cggaacgacg cgaaccccaa gttttgccga
     7561 ctcggcgttc gtgctagaag ccacctacaa ctcccatggc cctcgaccca cagatcaagt
     7621 ccaagatcga ccaactggtc gcccaagacc ccgtcgtcct gttcatgaag ggaacgcgca
     7681 gcttccccca gtgcgggttc tcggcctcgg tcgtgaacat cctcaacacc ttgatcccca
     7741 agtacgccac ggtcaacgtg ctatcggacc cggatgttcg gggaggcatg aaagagtact
     7801 ccgactggcc gacgttcccg cagctcttca tcaagggcga gttcgtgggc ggtgcgg
//
LOCUS       DASUFX010000171        17378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_305184,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000171 DASUFX010000000
VERSION     DASUFX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305184"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFV99_06830"
     CDS             <1..310
                     /locus_tag="VFV99_06830"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829605.1"
                     /note="catalyzes the formation of oxaloacetate from
                     L-aspartate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5059055.1"
                     /translation="LNWDEIRRFMWSYVGIVRSDKRLERARRRIEILRDEIREYYIDF
                     KVTSDLVELRNLALVAHLIIESARRRKESRGLHFTLDYPNKLPEARHSEIQLTDGPRG
                     "
     gene            555..1259
                     /locus_tag="VFV99_06835"
     CDS             555..1259
                     /locus_tag="VFV99_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059056.1"
                     /translation="MKRIAMVLALAACGPARGPSSPAADGVRAYLAALRSDDPHDAYA
                     MLTSDVKKKVSFQEFSIQWRESKQERAWQAKVLEESLKGNPDVGERALVSFSDGKRVT
                     LEREGKTWRLESELVTRSRAKEPRDAIRLFADAIAARDVGAALNLLTQRRRDGLTKQI
                     EGFISGLAKRVNQPFDNAFADRAELRWDENGIRYRIVLRKEEDEWRIDDIYIRPAPKE
                     EETNKPGEVEGIDDFQ"
     gene            complement(1260..3755)
                     /locus_tag="VFV99_06840"
     CDS             complement(1260..3755)
                     /locus_tag="VFV99_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX5059057.1"
                     /translation="MRALALVAVGLMSFAACGDNNEIPALPLVSGAAQLELAGDTLTF
                     SRSGTALLTFHTGAFQIGTVDDLDGDWSFDPYWLFVDAPQEPDGLAWHASGNFHVRRS
                     DVSQLLLAFDVPGGEATLSFTPGREGGFRAVFTTTAKNAAFLRIRPDADASEGFYGLG
                     EWADGIEHRGMLRPMQLEVDATIESTNNENHVPVPLLVGTRGWGLFAKSDRPGSFDVA
                     RQSDTLIDVTFGTGDDSDTGLEFHLFSAEQPLDVYKQYYDVAGYPGLPAEWAYGPLLW
                     RDENDNQAQVLDDIQQIRTRDLATSGIWFDRPYATGVNTFDFDATKFPAPETMLAALH
                     DAGLRYAIWQVPYVAAADRDDPAPAQNAYATEHGFFPPMIGVPVNQWGKPIDFTNPDA
                     YAWWKQNLSAYTLPLGGGGLGVEGFKLDYAEDIVLGLSGTRTPWQFADGSDERTMHYG
                     YTMLYHQIHRELLDTNGGFLLTRTGRWGDQTKGMIIWPGDLDADLTKFGDTPPGESRR
                     VVGGLPTALSFGLGLSASGFPFYASDTGGYRKSPPNNETWLRWVEANAVWPAMQVGDS
                     SSQMPWEFTAQNGRSQASLDAYRIYARLHMRLFPYVWTYAKQIAATGRPIARPYGLQY
                     HFENEQLSDQYVLGDYLMVAPVITAGQTSRAVKFPGASDWYDWWSGEHVALGTMAAAP
                     LDKLPLYIRGGGVVPMLRDTIDTLAPTTLNGVESYANDPGQLVVRTAPGADQAPFVMF
                     DGTTIEVTSGTRAIKLTQGTRFKQGVVFEVIAWSTAYSAPTAVNDASLGALTQRNSYT
                     ALQNATEGWFFDAAATSGTLWIKVPGAANITIQ"
     gene            complement(3758..4903)
                     /locus_tag="VFV99_06845"
     CDS             complement(3758..4903)
                     /locus_tag="VFV99_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059058.1"
                     /translation="MAEEPRPGLIVGGRYELIEIAGRGGMADVWRGRVRGDWGFVRDV
                     AVKQMHQNLASQPPYVAMFVEEARVGSALQSPNVAEVHDFVHDRGNYYMILEWIDGVD
                     LGTWIRWHVGRNEQTRWELIAAVAVGILRGLAAAHERVLPDGTLMPVVHRDVSPHNVL
                     LTNRGMVKLIDFGLALAPDRTQETTEPGIVKGKMSYLAPEIVAGGRPVPASDQFACGS
                     VLWEALVGRKLFEGSTDYETYTRLRDCMVQPLRPLRPDVPAPFVQIIHRALSAGIEQR
                     FPSTREMARQIGTTLKKVQLRKDLHTVLSKSVAEARQGMGLGPRTGDPSSATPVAQIM
                     ADDTPRIELSPKVETPPQGSPTVSQGSAMDKLRGLRHRLPFFGRKRG"
     gene            5015..5695
                     /locus_tag="VFV99_06850"
     CDS             5015..5695
                     /locus_tag="VFV99_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5059059.1"
                     /translation="MGREDFALDHAALLIGAWDYPERDLEGYRTMLDGIADRVQPDVA
                     RASNGIGRARAISDYLFDRLGYSGNADDYYDPRNSFLCEVIDRKRGIPISLSVLYLEV
                     ARRVGVLAQGVNFPGHFLVRVAIEDAWLFIDPFTQGRVLTPADLEQLLRKTTTPNAVL
                     EPSVIAAASKRQIVARMLVNLAGIYGRNGDLPRSLDVLERLAVLEPSNPRIARDLAQL
                     RERVVGLN"
     gene            5719..6861
                     /locus_tag="VFV99_06855"
     CDS             5719..6861
                     /locus_tag="VFV99_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5059060.1"
                     /translation="MSHEPFSPGSVARQLDYCTFCPKMCRHACPVSTASGRETYIPQV
                     KMDRLNQLRLGRSSWTTESTDPLWACTGCRHCTMYCDHGNEPGLVLLTGRAAAVDHGA
                     PHPALTGYSERFRAREQRLSALLVAGFPDQLASEGDVGYWPGCDAIDKGAADIAAAMA
                     LFERTGVDVKLVEAPQACAGYPLLAAGHRDLFRWHAGRVAAAMRGFRKVAINCSACLY
                     AVRAQYAAEGVTVRPEVVSLAELLAPAANSLACPLNRKSVYYHDPCYHARYNNVVEQP
                     RRALAQLAELREFSWHKTDTECCGGGGLLPKTMPLVADQMSKRRLEEVKNAGGGMVVT
                     SCATCTFMLKRNAPPSVEVANLATAMAQLSETPFTAPPSSPLDDEV"
     gene            7032..9134
                     /locus_tag="VFV99_06860"
     CDS             7032..9134
                     /locus_tag="VFV99_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059061.1"
                     /translation="MASVLGRYHVLKHLTSGGMAEVLLGRSDGIEGFERHVVLKRIRA
                     EHARDKRFISMFIDEARLAAGLHHQAIVQVFDIGETNGEYFFAMEYIHGEDLRRVLST
                     AAKGRAHVPLAYACSIVSAVAAGLHYAHERRDSRGKPLDIVHRDVSPSNILIGYDGAV
                     KIVDFGIAKAALRQAETVVGSLKGKTSYMSPEQCRGQSIDRRSDVYGLGVLLYELTTT
                     SRLFKGDNDYLVMDAIVNGKFAMPQMRRPDLPNELSSIIMRALAVEPERRYQTADELR
                     ADLDNFASRYDLTTGPSQLASYMRKLFGEKPEPWLELSNADTTEEMLPPETHRSWTDL
                     PSIEARDKRQQSLSEGRTSQQIAVDNERRRSSHHGSTESRRNSRGSVMPNVVAAVDTA
                     QTPKFSPYDTRPSMKLGWEAESPAPAPTRSPNKLLWAAVPVLALVAFALWKLGLDKDS
                     KQPPAAAKTTVVAMPAIPAPAPMPAAAPPPTRVVGPVVEEPAQVAAAPPMPEHKTIAK
                     QTPTKSVEKKAEVVEKKPDTEKRVAVEKKTEPAEKIVKKTAAAEPPSIPGIASTPAPT
                     PVAAVATAPLPVPTPAPAAAPAAVATPPAAPAITAAPAAKEIETTIPSLSNATVSAAA
                     SEHSRQLGACEGGVTLHGDVAVSFQIDGAGKVVHSQLSSTIKNPKVAACILKAVQSWK
                     FPRPVAGAAKGVYSISYQ"
     gene            9348..10817
                     /locus_tag="VFV99_06865"
     CDS             9348..10817
                     /locus_tag="VFV99_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059062.1"
                     /translation="MLSVIGLAFPTWFFVARLVLAFLALVGFLYLIQTWRHPRLGVAI
                     VFTSVETFGVSVLIALSAWLAAKQGERWEPVAALQLTMAAIPVIAPPFIWVGYVLLGG
                     FAAQLVLIELYQQYVGLGPFVGAEPFGSLCVPLAALGVLLVRKERQQIGLAYIRAQSE
                     AAALVRLAPVLASIREELGLANRALEVVALRSDRTPGALPRSIHRLDTLRDQLDALST
                     PIEPPHDGERDLLTRDAQYGAFVFALLGCIVLVFAGWSTHALPVGYSTEMSLAIAVGY
                     GFIALWLWLKRARPTIRRGVIACSLVLGSAMVLAVYMHSYWVTLDGLVFPFIGEKLIL
                     MILVVLRFPRLWIGALLIALIAASHIAIDAIFATAPIPLEPWHMLTFAAIGLGFLVMS
                     EQRRIAKLALLRAETIKSSLHRRANLSIALCDQLNTPLQTVLGIVELQPPTSATTEAF
                     ARIEQLRASSKRLARLVEELPPDLHRVSLDSAAHLRRRV"
     gene            complement(10825..11454)
                     /locus_tag="VFV99_06870"
     CDS             complement(10825..11454)
                     /locus_tag="VFV99_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5059063.1"
                     /translation="MSLTLYVGSKRYSSWSLRPYLALAHTGAQFDCKTILLDQNDTKA
                     NIAKVNPAGKVPVLHDDKLIVWDSLAICEYVNELYPDAKLWPADRAKRASARAISAEM
                     HSGFAPLRQNMPMDIGGKHPGKGHTPEALADARRVQAIWREALGASGGPFLFGAFSIA
                     DAMFAPVTTRFVTYGVDLDATCRAYVDAVYGLPGMKQWIADAAKEPQSR"
     gene            11568..13061
                     /locus_tag="VFV99_06875"
     CDS             11568..13061
                     /locus_tag="VFV99_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEX5059064.1"
                     /translation="MVTKASAKKAIPVDAQCPLSGASVHEDYDCMLNQTNIGANNNKF
                     YVIQLLEKGGKYYCWTRWGRVGEDGQNALIGPSDFGKALKAFQSKFKDKTKNAWEDRA
                     NFQPAAGRYTLIEIDRSVDDKKAAELEEKLKGIDADAAKVQKATAKKYAPCKLDAPVE
                     KFMNLIFDEDMFKGAMASFDIDVKKMPLGQLSKHQVEKGFEVLEELEDAIEKKKGKLI
                     ETVTSRFYTVIPHAFGRRVPPLIDSMELLQKKFDMLNVLNDIEIAVSVQKGDGKTSSK
                     LLPHPMDENYAGLNADIEYVDEKSAEYAFVDKYIDATARAGRKPKLINLFRLDRKAEG
                     KRFSAHDDIGHRKLLWHGTNVAVVAAICKTGMRIMPHSGGRVGKGIYLASENGKSINY
                     MGWASGGMGVMFLAEAALGKEHSITKDDSSLKAAPKGYDSIVARGQNEPDPKHDITFK
                     FEGKDVIIPQGKPIPQKQYAKSSFDQSEYLLYQESQVRIRYCCQVKI"
     gene            13061..14533
                     /locus_tag="VFV99_06880"
     CDS             13061..14533
                     /locus_tag="VFV99_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEX5059065.1"
                     /translation="MANVIEEVTLNWTDLTGAKTGATSLGSNKFYRAQIMSDFTVVFT
                     YGRVGQSGQVQTVKGKDLADAKKQLQKKIDSKIAKGYTKVELRSDQEEKKKAAAKAAA
                     GTNGATAAAPTKAKGPAKKSAFHPEVEELLGIIYNSTGNALASGLSSSAGATKDAPLG
                     NLSDAQLDKGADILDEIEKLVAGKPKNKELIDLTNDYLSNIPRQIDHARKGKKLDLDA
                     IVINSKERIAEQRQFIGLLRDAFLLKDVFADAAVVDDPHEVWYQGLKCDMDWIDQKSD
                     QYKEIKELFDEGQSPINSNFYGKLKVGRIWKLEQQGRKSRFEQYADSIAQKKDATGVT
                     PGWHGTRTENLMGICKTGLLMPQNLPKGVHVTGRAFGLGIYHTPRWPDSGGKTKDEKG
                     KTFTRYNGALKSCNYTSLKGAFYHRNNTSNTGFLFLEELALGVPELALEACFDKPAPK
                     KGCDYIYARAHGHASLSNDEVVTFDENASRRSAILEIIHK"
     gene            14546..15439
                     /locus_tag="VFV99_06885"
     CDS             14546..15439
                     /locus_tag="VFV99_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5059066.1"
                     /translation="MDVIRALLESRPPAVTTLATWWEATAVPRAAFATSLDRALVGGA
                     LADRLGFAFASAYGEALRALVPGTEGITALCITEAGGNHPKAIETKLVPAGDGYMISG
                     HKKWATVGSLASSLLVCATTGDHRLRMVRVPANAPGVTITGSSAPFVPEIPHAEITLD
                     HVAVRASDVLPGDGYDEYVKPFRTIEDAHVHAALLGYLVSVARRKGFAPDMIERLLAL
                     AVATRAAAAADVKAPTTHVALAGTFALVAREVAALEALWAALPDDEWERWQRDRMLLQ
                     VASKARDARRDKAWSLLRERG"
     gene            complement(15881..16468)
                     /locus_tag="VFV99_06890"
     CDS             complement(15881..16468)
                     /locus_tag="VFV99_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059067.1"
                     /translation="MNVRVGVPSSRGATAMTTIESIPYTYERLVQARAFRNARCYAPD
                     AVTTLLRGAMSAPDVALIVDVDTLERSALAKIDQVMLLALDALAHAGVHVVLVARYER
                     DRAAMLQARIRGAWCFDPSDGSVIHRLRQWRASAHTIVVTDDLALLAELGSADHGLAL
                     GRPELVRENIAALGDTSVRATLWWLLEQRRHGVAA"
     gene            16752..17306
                     /locus_tag="VFV99_06895"
     CDS             16752..17306
                     /locus_tag="VFV99_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059068.1"
                     /translation="MAAQFERIIRRVKSSMSYRGSYSTRDILGSLVARWLQSGEWDRL
                     KQLPPADRHIGESVRRFILDRIDQLRRRGDHEEMPEDLLVVPDEAMLTEIIEAAELRA
                     WIEARIAELEGGVVDPRVRIPLANVPQVGNALRLHMTGYTQRQIAGELGISLGAANKR
                     ILEGTNYLVVLQGIESGLAGGASA"
BASE COUNT         3224 a         5694 c         5578 g         2882 t
ORIGIN      
        1 gctgaactgg gacgagatcc gccggttcat gtggtcgtac gtcggcatcg tgcgttcgga
       61 caaacgcctc gaacgcgccc gccgccgcat cgagatcttg cgcgacgaga tccgcgagta
      121 ctacatcgac ttcaaggtca cgtcggacct ggtcgagctt cgcaacctcg cactcgtcgc
      181 acacttgatc atcgagagcg cacgccgccg caaggaatcg cgaggcctac atttcacgct
      241 cgactatccg aacaagcttc ccgaagcgcg tcattccgag atccagctga ccgacggccc
      301 gcgcggttag ccgtttcaca caacacggta tgaaccggcg ttgacacggt agccgggaat
      361 ggcacggcgg taggcccact tgcggccgtt gaatgccggc cggctggata cgtccatctg
      421 gcatctgcct cgcagtacct cgaggcatct actcctcgct gccggatctt gacggacggc
      481 cacgcagcgt aggggggcca ccggagcatg gtgctccggt ggctttgttt ttttcggcgc
      541 gctaatgtca cggcgtgaag cggattgcga tggttctggc gttagccgcg tgcggccctg
      601 cgcgtggtcc gtccagtcca gctgccgatg gtgtgcgtgc gtatctcgcc gcactccgca
      661 gcgacgatcc gcacgacgcg tacgcgatgc tgaccagtga cgtcaaaaag aaggtcagct
      721 ttcaggagtt ctcgatccag tggcgcgaga gcaagcagga gcgcgcgtgg caggcgaagg
      781 tgctggagga gagcctcaag ggcaacccag atgtcggtga gcgcgcgctc gtcagcttct
      841 cggatggcaa gcgcgtcacg ctcgaacgcg aaggcaagac gtggcgcctc gagtccgagc
      901 tcgtgacgcg atcgcgtgcg aaggaaccac gcgatgcgat ccggttgttc gctgatgcga
      961 tcgctgcgcg cgatgtcggc gccgcgctca atctgctcac gcagcgccgc cgcgacggcc
     1021 tgacgaaaca gatcgaaggc ttcatctccg gcctcgccaa gcgcgtcaat caaccgttcg
     1081 acaacgcgtt cgccgaccgc gccgagctgc gctgggacga gaacggcatc cgctaccgca
     1141 tcgtcctccg caaggaagaa gacgagtggc gcatcgacga catctacatt cgccccgcac
     1201 ccaaagaaga agagacaaac aagcccggcg aagtcgaagg catcgacgac ttccagtagc
     1261 tactggatcg tgatgttcgc ggcgccgggc accttgatcc acagcgtgcc gctcgtagcc
     1321 gccgcatcga agaaccagcc ttcggtcgcg ttctgcagcg cggtgtagct gttgcgctgc
     1381 gtgagtgcgc cgagcgacgc atcgttgacc gcggtcggcg cgctgtacgc ggtgctccac
     1441 gcgatcactt cgaacaccac gccctgtttg aagcgcgtgc cctgcgtgag cttgatggca
     1501 cgcgtaccgc tcgtcacctc gatcgtcgtg ccgtcgaaca tcacaaacgg cgcctgatcg
     1561 gcacccggcg ccgtacgcac gacgagctgg cccggatcgt tcgcgtacga ctcgacgccg
     1621 ttcagcgtgg tcggcgcgag cgtatcgatc gtatcgcgca gcatcggcac gacgccgccg
     1681 ccgcggatgt agagcggcag cttgtcgagc ggcgccgccg ccatcgtgcc gagcgcgacg
     1741 tgctcgccgg accaccagtc gtaccagtcg ctggcgcccg gaaacttcac cgcgcgcgac
     1801 gtctgtcccg cggtgatcac cggcgccacc atgaggtagt cgccgagcac gtattgatcg
     1861 ctcagctgct cgttctcgaa gtggtactgc agcccgtacg gccgcgcgat cggtcggccg
     1921 gtcgccgcga tctgtttcgc gtacgtccac acgtacggga acagccgcat gtgaaggcgc
     1981 gcgtagatcc gatacgcgtc gagcgacgcc tgcgagcggc cgttctgcgc ggtgaattcc
     2041 cacggcatct gactgctcga gtcaccgacc tgcatcgccg gccacaccgc gtttgcctcg
     2101 acccagcgca gccaggtctc gttgttcggt ggcgacttgc ggtagccgcc ggtatccgac
     2161 gcgtagaacg gaaagcctga cgccgacagc ccgaggccaa acgacagcgc ggtcggcaag
     2221 ccaccgacga cgcgcctcga ttcacccggc ggcgtgtcgc cgaacttcgt gagatcggcg
     2281 tcgagatcgc cgggccagat gatcatgccc ttcgtctgat cgccccagcg ccccgtgcgc
     2341 gtgagcaaga agccaccatt cgtgtcgagc agctcgcgat ggatctggtg atagagcatc
     2401 gtgtagccgt agtgcatcgt gcgctcgtcg ctgccatcgg cgaattgcca cggcgtgcgc
     2461 gtgcccgaca gaccgagcac gatgtcttcg gcgtaatcga gcttgaaacc ctcgacgccg
     2521 agaccaccac cgccgagcgg aagcgtgtac gcactgaggt tctgcttcca ccacgcgtac
     2581 gcatcgggat tcgtgaagtc gatcggcttg ccccactgat tgaccggcac accgatcatc
     2641 ggcggaaaga agccgtgctc ggtcgcgtac gcgttttgcg cgggcgccgg atcgtcgcga
     2701 tccgcggcgg caacgtacgg cacctgccag atcgcgtagc gcagaccggc gtcgtgcagc
     2761 gcggcgagca tcgtttccgg cgccgggaat ttcgtcgcat cgaagtcgaa cgtgttcacg
     2821 cccgtcgcgt acggtcgatc gaaccagatg ccgctcgtcg cgagatcgcg cgtgcggatc
     2881 tgctggatgt cgtcgaggac ctgcgcttgg ttgtcgttct cgtcgcgcca gagcaatggg
     2941 ccgtacgccc actcggcggg caatcccgga tagcccgcga cgtcgtagta ctgcttgtag
     3001 acatcgagcg gttgctcggc ggagaacagg tgaaattcga gaccggtgtc gctgtcgtcg
     3061 ccggtgccga acgtgacgtc gatcagcgtg tcggactgcc gcgcgacatc gaacgagccc
     3121 ggccgatcgc tcttcgcgaa caagccccag ccgcgagtac cgacgagcag cggcaccggc
     3181 acgtgattct cgttgttcgt cgattcgatc gtcgcgtcga cctcgagctg catcggccgc
     3241 aacatgccgc gatgctcgat gccatctgcc cactcgccga gaccgtagaa gccttcgctc
     3301 gcatcggcgt cgggacgaat gcgcaagaac gccgcgttct tcgccgtcgt ggtgaacact
     3361 gcgcggaagc caccctcgcg ccccggcgtg aacgacagcg tcgcttcgcc acccggcacg
     3421 tcgaacgcca gcagcagctg ggacacatcc gaacgacgca cgtgaaagtt accgctcgcg
     3481 tgccacgcga ggccgtcggg ctcttgcggc gcgtcgacga acaaccagta tggatcgaag
     3541 ctccaatcgc catcgagatc gtcgacggtg ccgatttgaa acgcgccggt atgaaacgtg
     3601 agaagtgctg tgccgctgcg cgagaacgtc agcgtgtcgc cggcgagctc gagctgcgcc
     3661 gcccccgaca cgagcggcag tgccggaatt tcgttgttgt caccacacgc cgcgaaggac
     3721 atcaagccga ccgccaccag agcgagcgcg cgcacgacta gccacgcttc cgcccgaaga
     3781 acggcaagcg gtgacgtagg ccgcgcagct tgtccatcgc gctgccctgg ctgaccgtcg
     3841 gcgagccttg cggcggtgtt tccacctttg gcgacagctc gatgcgcggc gtgtcatccg
     3901 ccatgatctg cgcgaccggt gtcgcgctcg acggatcgcc cgtgcgcggg ccaagcccca
     3961 tgccctgccg cgcttcggcg acgctcttcg acagcaccgt gtgcaggtct ttgcggagct
     4021 gcaccttctt gagcgtggtg ccgatctggc gcgccatctc gcgcgtcgac ggaaaccgtt
     4081 gctcgatgcc agccgacagc gcgcgatgaa tgatctggac gaacggcgcg ggcacgtcgg
     4141 gacgcagcgg ccgcagcggc tgcaccatgc agtcgcgcaa ccgcgtgtac gtctcgtagt
     4201 cggtcgagcc ttcgaacagc ttgcgaccga cgagcgcttc ccacagcacc gagccgcacg
     4261 cgaactgatc gcttgccggc accggccgac caccggcgac gatctcgggc gcgagatacg
     4321 acatcttgcc cttcacgatg ccgggctcgg tcgtctcctg cgttcgatcc ggcgcgagcg
     4381 cgagaccaaa atcgatgagc ttcaccatgc cgcgattcgt gagcagcacg ttgtgcggcg
     4441 atacatcgcg atggacgacc ggcatcaacg tgccgtcggg caacacgcgt tcgtgcgccg
     4501 ccgcgagccc gcgaagaatt ccgacggcga ccgctgcaat cagctcccag cgcgtctgct
     4561 cgtttcgacc gacgtgccag cggatccagg tgccgagatc gacgccgtcg atccattcca
     4621 ggatcatgta gtaattaccg cggtcgtgta cgaagtcgtg cacctcggcg acgttcggag
     4681 actgcaacgc cgatccaacg cgcgcttcct cgacgaacat cgcaacatac ggcggttgcg
     4741 atgcgagatt ctggtgcatt tgctttaccg cgacgtcgcg aacaaagccc cagtcacctc
     4801 tgacgcgtcc ccgccataca tccgccatgc caccacggcc agcaatttcg atgagctcgt
     4861 agcgtccgcc gacgattagc ccgggtcgtg gctcttcggc catggaaggg ctcatcttcg
     4921 catagtcgaa gcaaacaatc cgcgccgacg ccgtcgaatg gtaatctcgg gagctatgga
     4981 gcctagcccg ggtcttaccc ggttcagcga ggtggtgggt cgggaggatt tcgcgctgga
     5041 ccacgcagca ctgctgattg gcgcgtggga ttaccccgag cgcgatctcg aggggtaccg
     5101 cacgatgctc gacggtatcg ccgaccgcgt gcagccggat gtcgcgcgag catcgaacgg
     5161 tatcggccgc gcgcgcgcga tcagcgacta cctcttcgat cgccttggct acagcggcaa
     5221 cgcagacgac tactacgatc cgcgcaacag ctttctgtgc gaggtgatcg atcgcaagcg
     5281 cggcattccg atctcgctgt cggtgctcta cctcgaagtt gcccgtcgcg tcggcgtgct
     5341 cgcccaaggc gtcaacttcc ccggtcactt cctcgtccgc gtcgcgatcg aggatgcgtg
     5401 gctgttcatc gatccgttca cgcaaggccg cgtgctcacg cccgccgacc tcgagcaact
     5461 gttgcgcaag accacgacac cgaacgccgt gctcgagccg agcgtgattg ccgctgcgag
     5521 caagcgccag atcgtggccc gcatgctcgt caacctcgcc ggcatctatg gccgtaacgg
     5581 cgatttgccg cgttctctcg acgtcctcga gcgcctcgcc gtgctggaac cgtcgaatcc
     5641 gcgaatcgcg cgcgatctgg cgcagcttcg cgagcgagtc gttggtctga actaagccca
     5701 ctcgctatag tcggcgcaat gtcacacgag cccttcagtc cgggttcggt cgcgcgccag
     5761 ctcgactact gcacgttctg cccgaagatg tgtcggcacg cgtgcccggt gtcaacggcg
     5821 agcgggcggg aaacgtacat cccgcaagta aagatggacc gcctcaatca gctgcgcctt
     5881 ggccgcagct cgtggacgac cgagtcgacc gatccgctgt gggcgtgtac cggctgccgc
     5941 cactgcacga tgtactgcga tcacggcaac gagcctggcc tcgtgctgct cacgggccgc
     6001 gccgccgcgg tcgatcacgg cgcgccgcat ccagcactca ccggctacag cgaacggttc
     6061 cgcgcgcgcg agcaacggct ctctgcactg ctggtcgccg ggtttccaga tcagctcgcg
     6121 agcgaaggcg acgtcggcta ctggccgggc tgcgacgcga tcgacaaagg cgccgccgat
     6181 attgctgccg cgatggcgtt attcgagcgc accggcgtcg acgtcaagct cgtcgaggcg
     6241 ccgcaagcgt gcgccggcta cccgctactc gccgctgggc atcgcgatct atttcgctgg
     6301 catgcaggcc gcgtcgccgc cgcgatgcgc ggctttcgca aggtcgcgat caactgctcc
     6361 gcgtgtctct acgcggtgcg cgcgcaatac gctgccgaag gcgtcaccgt acgaccagag
     6421 gtcgtgtcgc tcgcagagct actcgcgccg gcggcgaaca gcctcgcctg cccgctcaat
     6481 cgcaagagcg tctactacca cgacccctgc tatcacgcgc gctacaacaa cgtcgtcgag
     6541 cagccgcgtc gcgcgctcgc gcaactcgcc gagcttcgcg agttctcgtg gcacaagacc
     6601 gataccgagt gctgcggtgg tggcggcctg ttgccgaaga cgatgccgct cgtcgccgat
     6661 cagatgagca agcgccgcct cgaagaagtg aagaatgccg gcggcggcat ggtcgtcacg
     6721 tcgtgcgcga cctgcacgtt catgctcaag cgcaacgcgc caccgtccgt cgaagtggca
     6781 aacctcgcca ccgcgatggc acagctctcc gagacgccgt tcaccgcgcc gccgagctcg
     6841 ccgctcgacg acgaagtgta accagcagaa aaactctggc gcgatctgtc atgcagatct
     6901 tgaatgccgc gaacgcgagc gctggacgcg tgcgccgatg tgcgagcgca agccttgcag
     6961 cgtgtgagct accagcaagg acccgacgcg accttgatcg tctcgacgac gattccatac
     7021 gatgagctgc aatggcctca gtcttgggtc gctatcacgt tctcaaacat ctgacgtcgg
     7081 gagggatggc ggaagtacta ctggggcggt ccgacggcat cgaaggcttc gagcgacacg
     7141 tcgtgctcaa gcgcatccgc gccgagcacg cgcgcgacaa gcgcttcatc tcgatgttca
     7201 tcgacgaggc gcgcctcgca gccggcctcc atcaccaggc catcgtccag gtgttcgaca
     7261 tcggcgagac gaacggtgag tacttcttcg cgatggaata catccacggc gaagatctgc
     7321 gccgagtcct gtccacggcg gcgaagggcc gcgcgcacgt accactcgcg tacgcgtgct
     7381 cgatcgtgtc ggccgttgcg gccggattgc actacgcgca cgaacgccgc gactcgcgcg
     7441 gcaagccgct cgacatcgtg caccgcgatg tgtcgccgtc gaacattctg atcggctacg
     7501 acggcgcggt gaagatcgtc gactttggca tcgcgaaggc ggcgctgcgt caggccgaga
     7561 ccgtcgtcgg cagcctcaag ggcaagacct cgtacatgtc gcccgagcaa tgcaggggcc
     7621 agtcgatcga tcggcgcagc gatgtctacg gcctcggcgt tctgctctac gagctcacga
     7681 ccacatcgcg cctgttcaag ggcgacaacg actacctggt gatggacgcg atcgtgaacg
     7741 gcaagttcgc gatgccgcag atgcggcgcc ccgatctgcc gaacgagctc tcctcgatca
     7801 tcatgcgcgc gctcgctgtc gaacccgagc gccgctatca gaccgccgac gagctgcgcg
     7861 ccgatctcga caacttcgcg agtcgctacg atctcacgac cgggccatcg cagctcgcga
     7921 gctacatgcg caagctgttc ggtgagaagc ccgagccgtg gctcgagctg tcgaacgcgg
     7981 atacgaccga ggagatgctg ccgccggaga cgcatcgcag ctggaccgat ctgccgagca
     8041 tcgaggctcg cgataagagg cagcagagcc tgagcgaggg acgcacctcg caacagatcg
     8101 cggtagacaa cgagcgccgc cgcagctcgc accacggctc gaccgagagc cgccgcaact
     8161 cgcgcggcag cgtgatgcca aacgtcgtcg cagctgtcga taccgcgcag accccgaagt
     8221 tctcgcccta cgacacgcga ccgagcatga agctcggttg ggaagcggag agcccagcgc
     8281 ccgcgccgac gcgtagcccg aacaagctgt tgtgggcggc tgtgccggtg ctcgcgctcg
     8341 tcgcgttcgc gctctggaag ctcgggctcg acaaggacag caagcagccg cccgccgcgg
     8401 cgaagacgac agtggtcgcg atgcccgcga tcccagcgcc cgcgccgatg cccgctgcag
     8461 cgccaccgcc gacgcgcgtg gtcggtccgg tcgtcgagga accggcacag gtcgccgccg
     8521 cgccaccgat gccggagcat aagacgatcg cgaagcagac gccgacgaag agcgtcgaga
     8581 agaaggcaga ggtcgtcgag aagaagccgg acaccgagaa gagggtggcg gtcgagaaga
     8641 agaccgagcc cgccgagaag atcgtgaaga agacggccgc cgccgagccg ccgtcgattc
     8701 ccggcatcgc gtcgacgcct gcgccgacgc ccgtggctgc cgttgcgact gcgcctcttc
     8761 cggtgcccac gcccgcgccg gcggctgcac ctgccgcagt cgctacaccg ccggctgcac
     8821 ccgcgatcac cgcggcgcct gctgcgaagg agatcgagac gacgatccca tcgctatcga
     8881 acgccacggt ctcggccgcc gcatcggagc actcgcgcca gcttggcgcg tgcgaaggcg
     8941 gcgtgacgct gcatggcgat gtcgcggtgt cgttccagat cgacggcgct ggcaaggtgg
     9001 tgcacagcca gctgtcctcg acgatcaaga acccgaaggt cgctgcctgc atcctcaagg
     9061 cggtgcagag ctggaagttc ccgcggcccg tcgcgggtgc tgcaaagggc gtctacagca
     9121 tctcgtacca gtgagagaac gccggggtag cgcgtagcga gcgcatccga attgtccgtc
     9181 gacgagccct ggtttccggt ggtggcaggc ggcttgctgg gtcaggttgt gtgccccgag
     9241 cccgagggga tccgcaaaac ccgcacacag tagagcggga actactggcc cgcgatagct
     9301 accgcagcca cgcgattctc acgctcctga tcatcatctg gatcggtgtg ctcagcgtga
     9361 tcgggctcgc gttcccgacg tggttctttg ttgcgcgcct cgtgctcgcg tttctcgcgc
     9421 tcgtcggctt tctgtacctc atacagacgt ggcgtcatcc gcgtctcggc gtcgccatcg
     9481 tcttcacatc ggtcgagacg ttcggcgtct cggtcttgat cgcgttgtcg gcctggcttg
     9541 ccgcgaagca gggcgagcgc tgggagccgg tggccgcact tcagctcacg atggcagcca
     9601 tccccgtcat cgcgccgcca ttcatttggg ttggctatgt gctgctcggt gggtttgccg
     9661 cgcagctcgt cctcatcgag ctgtaccagc agtacgtggg cctcggcccc ttcgttggcg
     9721 cggagccctt tggctcgcta tgcgttccgc tggccgcgct gggcgtgctg ctcgtgcgca
     9781 aggaacgtca gcagatcggg ctcgcctata tccgcgcgca atcggaggcg gccgcgctcg
     9841 tccgcctggc gcccgtactc gcctcgatcc gcgaagagct cgggctcgcg aatcgcgccc
     9901 tcgaggtggt cgcacttcgg tccgacagga cgccgggcgc gctcccgcgt tcgattcacc
     9961 gactggacac gcttcgcgat cagctcgacg cgttgagcac tcccatcgag cctccccacg
    10021 acggagagcg tgatctcctc acgcgcgacg cgcagtacgg cgcgttcgtc ttcgcattac
    10081 tcggctgtat cgttctcgtc ttcgcggggt ggagcacgca cgcactgccg gttggctact
    10141 cgaccgagat gtcgctagct atcgcagtcg gctacgggtt cattgcgctg tggttgtggc
    10201 tcaagcgtgc gcgtcccacg atccggcgcg gcgtcatcgc ctgcagcttg gtgctcggtt
    10261 cggccatggt gctcgcggtt tacatgcaca gctactgggt cacgctcgac gggctcgtgt
    10321 tcccgttcat cggcgagaag ctgatcctga tgatcctggt cgtcctgcgc tttccgcggc
    10381 tgtggatcgg tgcgttgctg atcgcactca ttgccgcctc gcacatcgcc atcgacgcga
    10441 tcttcgcgac cgcgccgatt ccgctcgagc cctggcacat gctgacgttt gccgcgatcg
    10501 gcctcggatt cttggtcatg agcgagcagc ggcggatcgc gaagctcgcg ctgctgcgcg
    10561 cggagactat caagtcgtcg ttgcaccggc gcgcgaacct gtcgatcgcg ctgtgcgatc
    10621 agctgaacac accgctgcag acggtgctcg gaattgtcga gctgcagcca cccacctctg
    10681 cgaccaccga ggcgttcgcc cggatcgaac agctgcgagc atcgagtaag cgcctcgcgc
    10741 ggctcgtcga ggagctgccg ccggaccttc atcgcgtttc gctcgacagc gcagcgcacc
    10801 tcaggcgccg cgtctaggcg agcattagcg cgactgcggt tccttcgcgg cgtcggcgat
    10861 ccactgcttc atgccgggca gcccgtagac agcgtcgacg tacgcgcgac acgtggcatc
    10921 gaggtcgaca ccgtaggtca cgaagcgcgt cgtcaccggt gcgaacatcg cgtcggcgat
    10981 cgagaacgcg ccgaacaaga acggaccacc gctcgcgccg agtgcctcgc gccagatggc
    11041 ctgcacgcgg cgggcgtccg cgagcgcctc gggcgtgtgg cccttgccgg gatgcttgcc
    11101 gccgatgtcc atcggcatgt tctggcgcag cggcgcaaag cccgagtgca tctccgcaga
    11161 aatcgcgcgg gcgctggcgc gtttggcccg gtcggccggc cacagcttgg cgtcggggta
    11221 gagctcgttg acgtactcgc agatcgccag cgaatcccag acgatgagct tgtcatcatg
    11281 cagtaccggg actttgccgg ccgggttcac cttggcgatg ttcgccttcg tgtcgttctg
    11341 gtccagcaag atcgtcttgc agtcgaactg cgctcccgtg tgcgcgagtg cgaggtaggg
    11401 acggagcgac cacgacgagt accgcttgct gccgacgtag agggtgagcg acatggccgc
    11461 gactctatct ccggattgac cgagcggtca cacagctgcg ccctgcagac accagtacaa
    11521 ctcccctcgc tgctacgatg gccgccgtcg cgaaacagag gggtgacatg gttacgaaag
    11581 cgtccgcgaa gaaggcaatt cccgtcgatg ctcaatgtcc gctcagcggt gcgtcggtcc
    11641 acgaggacta cgactgcatg ctcaatcaaa cgaacattgg agcgaacaac aacaagttct
    11701 acgtgattca gctgctcgag aagggcggga agtactactg ctggactcgc tggggccgcg
    11761 tcggtgaaga cggccagaac gcgctgatcg gaccgagcga cttcggcaaa gcgctcaagg
    11821 cgtttcagtc gaagttcaag gacaagacga agaacgcgtg ggaagaccgc gcgaactttc
    11881 agccggccgc cggtcgctac acgctgatcg agatcgatcg cagcgtcgac gacaagaagg
    11941 ccgccgagct cgaagagaag ctcaagggca tcgatgccga cgcggcgaag gtgcagaagg
    12001 cgaccgcgaa gaagtacgcg ccgtgcaagc tcgacgcccc ggtcgagaag ttcatgaacc
    12061 tcatcttcga cgaagacatg ttcaagggcg cgatggcgtc gttcgacatc gacgtcaaga
    12121 agatgccgct cggtcagctc tcgaagcacc aggtcgagaa gggcttcgag gtgctcgagg
    12181 agctcgaaga cgcgatcgag aagaagaagg gcaagctgat cgagacggtc acctcgcgct
    12241 tctacaccgt gatcccccac gcgttcggac gccgcgtgcc gccgctcatc gattcgatgg
    12301 agctgctgca gaagaagttc gacatgctga acgtgctcaa cgacatcgag atcgcggtca
    12361 gcgtgcagaa gggcgacggc aaaacgtcga gcaagctgtt gccgcacccg atggacgaga
    12421 actacgcggg cctcaacgcg gacatcgagt acgtcgacga gaagagcgcg gagtacgcgt
    12481 tcgtcgacaa gtacatcgac gcgacggcgc gcgccggccg caagccgaag ctcatcaact
    12541 tgttccgcct cgaccgcaag gccgagggca agcgcttctc ggcgcacgac gacatcggcc
    12601 accgcaagct gctctggcac ggcaccaacg tcgccgtcgt tgccgcgatc tgcaagaccg
    12661 gtatgcgcat catgccgcac tccggtggtc gcgtcggcaa gggcatctac ctcgcgtcgg
    12721 agaacggcaa gtcgattaac tacatgggct gggcgtccgg cggcatgggc gtgatgttcc
    12781 tcgccgaagc ggcgctcggc aaagagcact cgatcacgaa ggacgactcg tcgctgaagg
    12841 cggcgccgaa gggttacgac tcgatcgtcg cgcgcggcca gaacgagccc gacccgaagc
    12901 acgacatcac gttcaagttc gaaggcaagg acgtgatcat tccgcagggc aaaccgattc
    12961 cgcagaagca gtacgcgaag tcgagcttcg atcagagtga gtatctgctc taccaggaga
    13021 gccaggtccg cattcgttac tgctgccagg tgaagatcta atggcgaacg tcatcgagga
    13081 ggtcaccctc aactggaccg acctgaccgg cgccaagacg ggcgcgacgt cgctcggctc
    13141 gaacaagttt tatcgcgcgc agatcatgag cgacttcacg gtcgtcttca cgtacgggcg
    13201 cgtcgggcag agcggccagg tccagacggt gaagggcaag gacctcgccg acgccaagaa
    13261 gcagctgcag aagaagatcg actcgaagat cgcgaagggc tacacgaaag tcgagctccg
    13321 cagcgatcaa gaggagaaga agaaagcggc agcaaaggcg gcggctggaa cgaacggcgc
    13381 gacggccgca gcgcctacga aggcgaaggg ccccgcgaag aagagcgcgt tccatcccga
    13441 ggtcgaagag ctgctcggca tcatctacaa ctcgactggt aacgcgctcg cgtcaggtct
    13501 gtcgtcgtcg gcgggcgcga cgaaggatgc gccgctcggc aacctatccg atgcccagct
    13561 cgacaagggc gccgacatcc tcgacgaaat cgagaagctg gtcgcgggca agccgaagaa
    13621 caaagagctg atcgacctga cgaacgacta cctgtcgaac attccgcgtc agatcgatca
    13681 cgcgcgcaaa ggcaagaagc tcgatctcga cgcgatcgtg atcaactcga aggagcggat
    13741 cgccgagcag cgtcagttca tcggcctctt gcgcgacgcg ttcctcctca aagacgtgtt
    13801 cgccgacgcc gctgtcgtcg acgatccgca cgaggtctgg taccagggcc tcaagtgcga
    13861 catggactgg atcgatcaga agagcgacca gtacaaggag atcaaagagc tgttcgacga
    13921 gggccagtcg ccgatcaact cgaacttcta cggcaagctc aaggtcggcc ggatctggaa
    13981 gctcgagcag caaggccgca agtccaggtt cgagcagtac gcggactcga tcgcgcagaa
    14041 gaaggacgcg acgggtgtca ctccgggctg gcacggcacg cggaccgaga acctcatggg
    14101 tatctgcaag accggcctgc tcatgccgca gaacctgccg aagggcgtgc acgtcaccgg
    14161 gcgcgcgttc ggcctcggca tctatcacac gccgcgttgg ccagactccg gcggcaagac
    14221 gaaggacgag aagggcaaga ccttcacgcg ctacaacggc gcgctgaaga gctgcaacta
    14281 cacgtcgctg aagggcgcgt tctatcaccg caacaacacg tcgaacacgg gcttcttgtt
    14341 cctcgaagag ctcgcgctcg gcgtccccga gctcgcgctc gaagcgtgct tcgacaagcc
    14401 ggcgccgaag aagggctgcg attacatcta cgcccgcgcg cacggccacg cgtcgctgtc
    14461 gaacgacgaa gtcgtgacgt tcgatgaaaa cgcgtcacgt cgctcggcga ttctcgaaat
    14521 cattcacaag tagtaagaac gaggcgtgga cgtcatccgc gccttgctcg agagtcggcc
    14581 gccggcggtc accacactgg ccacttggtg ggaggcgacg gccgtgccgc gcgcggcgtt
    14641 cgccacctcg ctcgatcgcg cgcttgtcgg cggtgcactc gccgatcggc tcggcttcgc
    14701 gttcgcgagt gcttatggcg aagcgctgcg cgcgctcgtg cccggcacgg agggcatcac
    14761 tgcgctctgc atcaccgaag ccggcggcaa tcatccgaaa gcgatcgaga cgaagctcgt
    14821 acccgcgggc gatggctaca tgatttccgg ccacaagaag tgggcgacgg tgggctcgct
    14881 cgcatcgtcg ctgctcgtgt gtgcgacgac cggcgaccat cgactgcgca tggtgcgcgt
    14941 gcccgcgaac gcgccaggcg tgacgatcac aggcagcagc gcgccgttcg tgcccgagat
    15001 cccgcacgcc gagatcacgc tcgaccatgt cgcggtacgc gcgagcgacg tgctaccagg
    15061 cgatggctac gacgagtacg tgaagccgtt ccgcacgatc gaggacgcgc acgtgcacgc
    15121 cgcactgctc ggctatctcg tgagtgtcgc gcgccgcaag ggcttcgcac ccgacatgat
    15181 cgagcgcctg ctcgcactcg ccgtcgcgac gcgcgcagcg gctgccgccg atgtgaaggc
    15241 accgacgacg cacgtcgcgc tcgccgggac gttcgcgctc gtcgcgcgcg aggtcgctgc
    15301 gctcgaagcg ctgtgggccg ccctgcccga cgacgaatgg gaacgctggc agcgcgaccg
    15361 catgctgtta caagtcgcga gcaaggcacg cgacgcccgc cgcgacaagg cgtggtcgct
    15421 actccgcgaa cgcggttagc gcacgctcta tgaagagcga gcgcggcggt ccaccagagc
    15481 cgtcgcgact acctgtcgta ggatcgccgg cgatgctggc ttgacgagac acgcatcata
    15541 gtctgccgtg taccggtcga ccgctatgct ggacccggtg aatgcgatca acaccagatc
    15601 gtccccgctc gaacggagct cgcgggcaag cgagcagccc gtgccatcgt cgaggtgatg
    15661 atcgaggatc acgacgtgag gtcggtacgt acgcgcgagc tccagtgctt cgcgacgggt
    15721 ggtggcgagc ctgcagtcgt acccaacgga agcgaacacg ctcgagatca cgtccgcgag
    15781 ctcgtagtca tcgtctacaa cgagcacgcg tgcattcgcg ggcgccatgt tcaagtccga
    15841 gtgactgccg gacgcggaca ggctcgacga gcgcggcttg tcaggctgcc actccatgtc
    15901 gacgctgctc cagtaaccac catagcgtcg cccggacgga cgtatcaccg agcgccgcaa
    15961 tgttctcgcg aacgagctcc ggccttccga gcgcgagtcc gtggtccgcg ctgccgagct
    16021 cggcgagaag tgccaagtcg tccgtgacga caatcgtgtg cgcgctcgct cgccactgac
    16081 gcaaacgatg gatgaccgag ccatcgcttg gatcgaagca ccaggcgcca cgaatgcgag
    16141 cttgcagcat ggcggcgcgg tcgcgctcgt atctggccac gagcacgaca tgcacgccgg
    16201 catgtgcgag ggcatcgagg gctagcaaca tcacctggtc gatcttggcc agcgcgctcc
    16261 gctcgagcgt gtcgacgtca acgatcagtg ccacatcggg tgcgctcatc gcgccgcgga
    16321 gcaacgtcgt gacggcatca ggggcgtaac accgggcgtt gcggaacgcg cgcgcttgaa
    16381 cgaggcgctc gtatgtatac ggaatggact cgatggtggt catagcggtt gcccccctgg
    16441 aagacggcac gccgacgcgg acgttcatcg acgcccgcaa catcgcgatc tgcaaccact
    16501 agttcactag ctgcagatcg gctctccggt cgcgctgcca gccgacctcg tccgagttgc
    16561 accacaaaag gttgcagtac caagcattct ccaaggcgtg gtggtggggg gcgtgccaga
    16621 aaggacgcac caccatggga tacgttcacg gtgttatggt gatgaacaga ggagctcgac
    16681 cgacgtcctc acgatcagac gtcggcgtgt cgccgatggc tgaacgaacg gaatcaacgt
    16741 ccgatgacca gatggccgca cagttcgagc ggatcattcg gcgggtcaag agctcgatga
    16801 gctaccgcgg ctcgtacagc actagagaca tcctcggctc gctcgtcgcg cgctggttgc
    16861 agagcggcga atgggaccgg ctcaagcagc tacctccggc ggatcgacat atcggtgaga
    16921 gcgtgaggcg cttcatactc gacaggatcg atcagctgcg gcggcgtggg gatcacgaag
    16981 agatgccgga ggatctgctg gtcgttccgg atgaagcgat gctcactgaa atcatcgagg
    17041 cagccgagct ccgcgcgtgg atcgaggcac gcatcgcgga gctcgagggc ggcgtggtcg
    17101 atccgcgggt gcgaattcct ctcgccaacg tgccgcaggt cggtaacgca cttcgcctcc
    17161 atatgacggg gtacacacag cgtcagatcg ccggcgagct cggcatctcg ctgggggctg
    17221 ctaacaaacg aatcctcgag ggcacgaact atctcgtggt tctgcaagga atcgaatccg
    17281 gcctggcggg aggagcaagc gcgtgaccga cgcgcaggta cgagagaaac tcgcccgcat
    17341 ctctgctcga cctccggcgg agtggaccaa gcgcgtcg
//
LOCUS       DASUFX010000172         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1138733, whole genome shotgun sequence.
ACCESSION   DASUFX010000172 DASUFX010000000
VERSION     DASUFX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138733"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFV99_06900"
     CDS             complement(<1..900)
                     /locus_tag="VFV99_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX5059069.1"
                     /translation="MPPRVLVVDKSSSTRDVIADLLGDHASEVAWVSRDGALGFVRGG
                     QPFDVIVVDTDPPPTPALVRELASAGGNARVVLIASGVEGIALATKCGAADVVARPFV
                     AAELAFRVGRVTPGDRGVKRPRKSDVIIGTGSWIKELYDRIAMVAATDVTVAIFGESG
                     TGKELVARTIHNSSPPHDAPFVVVNCAAIPETLLEDELFGHVRGAFTDATRDREGLLA
                     AAHTGTLFLDEIGELPLPLQAKLLRVLQSQEFRRIGDDQDRRVDVRIITATNRNLDEL
                     VARGSFRQDLYYRINVFPMHLPPL"
     gene            complement(980..1933)
                     /locus_tag="VFV99_06905"
     CDS             complement(980..1933)
                     /locus_tag="VFV99_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059070.1"
                     /translation="MRRWLAPMLLLALAGWEPFRSANPDVEAGNAAFAAGDIAKALEA
                     YDRAAHDGTVDPAGLAYDRGTAELKQAEGIKDQKQHDAMVERGLESLKAAATSRDPRI
                     RGQANYNRGNTLLGQDKLDDAIESYKQALKDQPQLDDARMNLELALKRRQKKEQQQQQ
                     QGQQGQQGQQGQQGQQGQQGQQGQQGQQGQQGQQGQQGQQGQQGQPGQQGQQGQQQGQ
                     QGQQQQQQGQGQGQSQQKQNGQGQGSNDPQQGSGQGSNDQQNQNQQNSQRQHAGRTQP
                     QGPKTPTDGKLDDLEDYSRQLQKDGARRRASGRAKDPDQDW"
     gene            complement(1960..2859)
                     /locus_tag="VFV99_06910"
     CDS             complement(1960..2859)
                     /locus_tag="VFV99_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5059071.1"
                     /translation="MVALDVSKSMLVDDVGATREMQDKDIKPNRLARARELATALVDE
                     LPEDRIGPVVFAGAASHFPVTEDHQVAVRFLYDLGPADLPPGSNLAEVFRVSRCLLRP
                     DLYDDLGCARIGRRGHGGDALAGESLDPVDKKNDDIVEEKVERGKAIVIFTDGGDSDT
                     EALREVATSRELGIAVFVVGIGTTQGGVVHEMDYEGRPTATPKHAKDGSTVVSKRDDA
                     AMKLLAQAGGDPKRYFIASERGEVDPMPIVDALRAVNRGLATKKVDEKKDIFQPFLFA
                     GLMLLVIEAAISTRRRRKYPEEH"
BASE COUNT          411 a         1035 c         1018 g          584 t
ORIGIN      
        1 cagcggcggc aagtgcatcg ggaacacgtt gatgcggtag tagaggtcct ggcggaagct
       61 gccacgcgcg acgagctcgt cgaggttgcg gttcgtcgcg gtgatgatgc ggacgtcgac
      121 gcggcgatcc tgatcgtcgc cgatgcggcg aaactcctgc gactggagca cgcgcaagag
      181 cttggcctgc agcggcagcg gcagctcacc gatctcgtcg aggaacagcg tgccggtgtg
      241 ggccgcggcg agcaggcctt cgcgatcgcg cgtggcgtcc gtgaatgcgc cgcgcacgtg
      301 accgaacagc tcgtcttcga gcaacgtctc ggggatcgcc gcgcagttga cgacgacgaa
      361 cggcgcgtcg tgcggcggcg acgagttgtg gatcgtgcgc gcgaccagct ccttgccggt
      421 gccgctctcg ccgaagatcg cgaccgtgac gtcggtcgcg gcgaccatcg cgatgcggtc
      481 gtacagctcc ttgatccacg agccagtgcc gatgatgacg tcgctcttgc gcggccgctt
      541 gaccccacga tcaccaggcg tgacgcggcc gacgcggaac gcgagctcgg cggcgacgaa
      601 cgggcgcgca acgacgtcgg cggcgccgca cttcgtcgcg agcgcgatgc cctcgacgcc
      661 gctcgcgatt agcacgacgc gcgcgttgcc gccggccgac gcgagctcgc gcacgagcgc
      721 gggggtcggc ggcggatcgg tgtcgaccac gatcacgtcg aacggctgac cgccgcggac
      781 aaagccgagt gcgccgtcgc gcgaaaccca cgccacctcg ctggcgtgat caccgagcag
      841 atcagcgatc acgtcgcgcg tcgacgagct cttgtcgacg acgagcaccc tcggcggcat
      901 cccgatgatt gtaccccact gccttgcgcg tcggacttat cgcctcgggt agcgccatag
      961 gcattgtggc tgacgctcgc taccagtctt gatcaggatc tttcgcgcgc ccgctcgcac
     1021 gccggcgtgc gccgtctttc tgcaattggc gcgagtaatc ttcgagatcg tcgagctttc
     1081 cgtcggtggg cgtcttgggc ccctgcggct gcgtgcggcc agcgtgctgg cgctggctgt
     1141 tctgctggtt ctggttctgc tgatcgttcg atccctggcc agagccctgc tgcggatcgt
     1201 tcgatccttg tccctggccg ttctgcttct gctgactttg cccttggcct tggccctgtt
     1261 gctgttgctg ttgaccttgc tggccttgct gctggccttg ttggccttgt tggcccggtt
     1321 gcccttgctg gccctgctgc ccttgctggc cctgctgacc ctgctgaccc tgctgacctt
     1381 gttgtccctg ctggccttgc tgtccctgct gaccttgttg cccctgctgg ccttgttgcc
     1441 cttgctggcc ttgctgctgt tgctgctgtt ccttcttctg ccggcgcttg agcgcgagct
     1501 cgaggttcat gcgcgcgtcg tcgagctgcg gctgatcctt gagcgcttgc ttgtacgact
     1561 cgatcgcgtc gtcgagcttg tcttggccga gcagcgtgtt gccgcggttg tagttcgcct
     1621 ggccgcggat gcgcggatcg cgtgacgtcg ccgcggcctt gaggctctcg aggccgcgct
     1681 cgaccatcgc gtcgtgctgc ttctggtcct tgatgccttc ggcctgcttc agctccgcgg
     1741 tgccgcgatc gtaggcgagg cccgccggat cgacggtgcc gtcgtgcgcg gcgcggtcat
     1801 acgcctcgag cgccttggcg atgtcacccg cggcaaacgc cgcgttgccg gcttcgacgt
     1861 ccgggttcgc cgatcgaaac ggctcccagc ccgcgagcgc gaggagcagc atcggcgcga
     1921 gccagcggcg catgggtcga gcatgacacg cccgcgtcat cagtgctcct ccggatattt
     1981 gcgccgccga cgggtcgaaa tcgccgcctc gatgacgagc agcatcaagc ccgcgaacaa
     2041 gaacggctgg aagatgtcct tcttctcgtc gaccttcttc gtcgccaagc cgcggttgac
     2101 cgcgcgcagc gcgtcgacga tcggcatcgg atcgacctcg ccgcgctcgg acgcgatgaa
     2161 gtagcgcttg gggtcgccac ccgcctgggc gagcagcttc atcgcggcgt catcgcgctt
     2221 cgacacgacg gtcgagccgt ctttcgcgtg cttcggcgtc gcggtcggcc gaccctcgta
     2281 gtccatctcg tggacgacac cgccttgcgt cgtgccgatg ccgacgacga acaccgcgat
     2341 tccgagctcg cgtgacgtcg cgacctcgcg cagcgcctcg gtgtcggagt cgccaccgtc
     2401 ggtgaagatc acgatcgctt tgccgcgctc gaccttctcc tcgacgatgt cgtcgttctt
     2461 cttgtcgacg ggatcgaggc tctcgccggc gagcgcatcg ccgccatggc cacgcctacc
     2521 gatgcgcgcg cacccgaggt catcgtagag gtccgggcgg agcaagcagc gcgacacgcg
     2581 gaacacttcg gcgaggttcg agcccggcgg caggtcagcc ggcccgaggt cataaaggaa
     2641 gcgcaccgcg acctgatgat cttcggtgac cgggaagtgc gaggccgcgc ccgcgaacac
     2701 gaccggaccg atgcgatcct cgggcaattc gtcgaccagc gcggtcgcca gctcgcgcgc
     2761 gcgggcaagc cggttcggct tgatgtcctt gtcttgcatc tcacgcgtcg cgccgacatc
     2821 gtcgacgagc atcgacttcg agacgtcgag cgcgaccacg aggtcgaggc cgtgcagctc
     2881 gactttgcgt gtgccggcga tctgcggccg cgcgagcgac tatgccgcgt tcgatcgcgg
     2941 cttccagacc gtgcgcacga agctcgacac cacgaagcgc acggtcagcg agcgcgtgcc
     3001 ggatcagcaa ctgttcgtgc cgttcgcaac gctcgcggcg ttgctgat
//
LOCUS       DASUFX010000173         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1694763, whole genome shotgun sequence.
ACCESSION   DASUFX010000173 DASUFX010000000
VERSION     DASUFX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694763"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFV99_06915"
     CDS             complement(<1..347)
                     /locus_tag="VFV99_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5059072.1"
                     /translation="MLAEKWPDAVCELDHQNPYQLLVATILSAQSTDKMINTITPALF
                     AKYPDARALAAANPAELEQMIFKSGFYRNKTKSLIGMAKAVVENHDGEIPQTMEALVE
                     LPGVARKTANVVLG"
     gene            436..1359
                     /locus_tag="VFV99_06920"
     CDS             436..1359
                     /locus_tag="VFV99_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059073.1"
                     /translation="MAGRHRIIFCAALLAGACGNKKDVEHAKHSLYDTDFAIVYNTAL
                     SVTRGQYPTLNENPGPGKISTAWHQVSMASNQDDLSNPRTLAQQQGVQTGAGASAGMI
                     GAPTRLAYKRYFIRFDVTVLGGRPWRVKVVGHASEWDPGAALPSELRGAERPSWLDGR
                     TESLMVAIYKQLRKFAVPMKEEVEVTGEDDLPKTDPSTFTGVPAGAAKRLASLKDALA
                     KRDYTALRPQLDENVVWSLGGGTGADVALATWQADPGTFEAMVDVINGGCAAKGDKEV
                     RCPAGEPQAGKYQLVIEAKGDWKVTSFVRGE"
     gene            complement(1360..1893)
                     /locus_tag="VFV99_06925"
     CDS             complement(1360..1893)
                     /locus_tag="VFV99_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059074.1"
                     /translation="MRPVLVLLLAAACSDSSSMDTVDASGGGMPDTYDSARCLIKGDY
                     GAVGSLAGSATMGANTLSATLDARPPRDSFFLKLTAGKGVFTGGVATGTFTISGVDAG
                     YLTCGLCVHIIADIVAGSGPSKFYFADSGTVTLTSTAPIMGSATNLHFTAVDINSGAK
                     IADGCDATMASIMFANP"
     gene            complement(1956..2441)
                     /locus_tag="VFV99_06930"
     CDS             complement(1956..2441)
                     /locus_tag="VFV99_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059075.1"
                     /translation="MRCLLACVVVLALAQPRHAAAEDHGDIDPGTVYVLGGLYLGGTA
                     AFAIKDIAGSKPGMNYGIGEVLWNGTFTVLWTRFIISDALDGLSSHSGLPAASIFGGA
                     NAALLAHGIYTIVRNARHKTDAPPRNQGPPGAMQLGPVTAVVAPAAMQDGAGLGLAGT
                     F"
     gene            complement(2518..>4403)
                     /locus_tag="VFV99_06935"
     CDS             complement(2518..>4403)
                     /locus_tag="VFV99_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5059076.1"
                     /translation="VHNEAAAAILRKADRARGWSLSPQLQKLAVAWVEAHGLAGQHVR
                     GEGPMMMFAPLVAAVRELAPALGRVLGGGLQAHEATALVVDGLHVLNACDTAAVREGL
                     LDDVLLGKLARVRDQLTIVSAQAPWTDPYAALAALAPPLDRKRLAERLRNLRAGRQQA
                     GEPAASVDAAVATLGDQDLAVLGPALQTCQLWYCESATSGLSPAAQLRLLAAAVGAAR
                     KIGVDVSRPWHAQLRPLIARLHGDSSAVRYRLRLVEAALAQRSFAELLAGSAGLGPLG
                     TLSMKLASATDPDAVVIDYVDTDESAALVTLLGLYETRSQVAFHQMLKALCDLYGLRK
                     DELDRVNNEAAYLASMNAAKTDKERMLEYVRPGRIVEIGPGGGVVLDLLEAKFPSSEI
                     IGVDLSQEAVAALEAHARAGNHKWRVALGAAEVLPELVGSASVDTVVFCSILHEVYSY
                     TERGGTKFHLESVRDVIRAAWTTLKQGGRIVIRDGVMPLPGKRRIRFIAPDSRSTFDL
                     YVMQFEGRRIAFRELASDRVELSTADAMEFLYTYTWGPASFPYEVRELYGILPYDDYV
                     TAIVDWVGADAAKVVEIPTHLRSYLQPGYRDNLAGKIELTDEHDRAAELPDSNCLIVV
                     EKR"
BASE COUNT          745 a         1443 c         1493 g          722 t
ORIGIN      
        1 ccgaggacca cgttcgccgt cttgcgcgcg acgcccggca gctcgacgag cgcctccatc
       61 gtctggggga tttcaccgtc gtgattctcg acgacggctt tcgccatgcc gatcaggctc
      121 ttcgtcttgt tccggtagaa gccgctcttg aagatcatct gctcgagctc ggccggattg
      181 gccgctgcga gcgcgcgcgc gtcgggatat ttcgcgaaca gcgccggcgt gatcgtgttg
      241 atcatcttgt ccgtggattg cgcggacagg atcgtcgcga ccagcagctg ataggggttc
      301 tgatggtcga gctcgcagac cgcgtccggc catttctcgg caagcaaggc ggcaatctgc
      361 gcggcattct cgggggtagc ggtcgcaagg ccggcacgac gcggggctgg cgtgctattc
      421 cgtcggtgac ctcccatggc tggccggcac cgtatcattt tctgcgctgc gctcctcgcc
      481 ggcgcgtgcg gcaacaagaa ggacgtcgag cacgcgaagc actcgctgta cgacaccgat
      541 ttcgcgatcg tctacaacac cgcgctcagc gtcacgcgcg gccagtatcc gacgctgaac
      601 gagaatccgg gcccgggaaa gatctcgacc gcctggcacc aggtgtcgat ggcgagcaac
      661 caggacgatc tgtcgaaccc gcgcacgctc gcgcagcaac agggcgtgca gactggcgcg
      721 ggtgcgagcg cgggcatgat cggcgcgccg acacgactcg catacaagcg ctacttcatc
      781 cgcttcgacg tcaccgtgct cggtggccgc ccgtggcgtg tgaaggtcgt cggtcacgca
      841 tcggaatggg atccgggcgc ggcattgccg tcggagctgc gcggtgccga gcgcccgtcg
      901 tggcttgacg gtcgcaccga gtccttgatg gtcgcgatct acaaacagct gcggaagttc
      961 gcggtgccga tgaaggaaga ggtcgaggtc accggtgagg acgatctacc gaagaccgat
     1021 ccgtcgacgt tcaccggtgt gccggccggc gcggcgaagc gactcgcgtc actcaaggac
     1081 gcactcgcga agcgcgacta tacggcgctg cgcccgcagc tcgacgagaa cgtcgtgtgg
     1141 agtctcggcg gcggcaccgg tgccgacgtt gcgctcgcga cctggcaagc cgatccgggg
     1201 acgttcgaag cgatggtcga tgtgatcaac ggcggctgtg cggcgaaggg cgacaaagaa
     1261 gtgcgctgcc ccgcgggcga gccgcaagcc ggcaagtatc agctcgtgat cgaagcgaag
     1321 ggcgactgga aagtcacctc gttcgtccgc ggcgaatagc tacggattcg cgaacatgat
     1381 cgacgccatc gtcgcgtcgc aaccgtccgc gatcttcgcg ccgctgttga tgtcgaccgc
     1441 ggtgaaatgc aggttcgtcg ccgagcccat gatcggtgcg gtcgacgtca gcgtcaccgt
     1501 gccgctatcc gcgaagtaga acttcgacgg ccccgagccc gcgacgatgt ccgcgatgat
     1561 gtgaacgcac aagccgcacg tcagatagcc ggcgtcgacg ccgctgatcg tgaacgtgcc
     1621 tgtcgcgacg ccgccggtga acacgccctt gccagcggtc agcttgagga agaacgaatc
     1681 gcgcggcggc ctggcatcga gtgtggccga cagcgtgttc gcgcccatcg tcgcgctacc
     1741 ggctagcgag ccgaccgcgc cgtagtcacc tttgatcagg cagcgcgcgg agtcgtaggt
     1801 atcgggcatt ccgccgccgg atgcatcgac cgtgtccatc gacgacgaat ccgagcaagc
     1861 agcagcgagc agcagcacga gcacgggtcg catgcagaca atcgtctcac agccgccggt
     1921 gcgaggtgct cgcagaacat ctcacgcgct gacagctaga acgtgccggc gagcccgagt
     1981 ccggcgccgt cttgcatcgc tgcgggtgcg acgacggcgg tcactggacc gagttgcatc
     2041 gcgccggggg gaccctggtt gcgaggcggc gcgtcggtct tgtggcgcgc gttcctcacg
     2101 atcgtgtaga tgccgtgcgc gagcagtgcc gcattagccc cgccgaaaat ggaggccgcc
     2161 ggtagtccgc tgtggctgct cagcccgtcg agcgcgtcgc tgatgatgaa acgcgtccac
     2221 agtacggtga acgtgccgtt ccacaggacc tcgccgatgc cgtagttcat gccgggcttg
     2281 ctgccagcga tatctttgat cgcgaacgcg gcggtgccgc cgaggtacag gccgccgagc
     2341 acgtagaccg taccgggatc aatgtcgcca tgatcttccg cagcggcgtg acgcggctgc
     2401 gcgagcgcga gcacgacgac acaagcaagc aagcaccgca tgacctgatg gtgcccatgg
     2461 gcagatgtag gtcggaaacg gttcacgcgt tgttcacgcg cggcaatcgc cggcaaatca
     2521 ccgcttctcg acgacgatca agcagttcga gtcgggcagc tcggccgcac gatcgtgctc
     2581 gtcggtcaat tcgatcttgc cggcgaggtt gtcgcggtaa ccgggctgga ggtagctgcg
     2641 caggtgcgtg ggaatctcga cgaccttcgc ggcgtcggcg ccgacccagt cgacgatcgc
     2701 cgtgacgtag tcgtcgtacg gcaggatgcc gtagagctcg cgcacttcgt acgggaagct
     2761 cgccggcccc cacgtgtacg tgtagagaaa ctccatcgcg tctgcggtcg acagctcgac
     2821 gcgatcggag gcgagctcgc gaaacgcgat gcgcctgcct tcgaactgca tcacgtagag
     2881 atcgaacgtc gagcgcgagt cgggcgcgat gaagcggatg cgccgcttgc ccggcagcgg
     2941 catcacgccg tcgcggatca cgatgcgccc accttgcttc agcgtggtcc acgccgcgcg
     3001 gatcacgtcg cgcacgctct cgagatgaaa cttggtgccg ccgcgttcgg tgtacgagta
     3061 gacctcgtgc aggatcgagc agaacacgac ggtgtcgacg ctcgcgctgc cgactagctc
     3121 gggcagcact tcggccgcgc caagcgcgac gcgccatttg tgattgccgg cgcgtgcgtg
     3181 ggcttcgagt gcggcgactg cttcctgcga gagatcgacg ccaatgattt cagagctcgg
     3241 gaacttcgct tcgagcagat cgagcaccac gccgccgccg ggcccgatct cgacgatgcg
     3301 gccgggccgc acgtactcga gcatgcgctc cttgtcggtc ttcgccgcgt tcatcgacgc
     3361 gaggtacgca gcctcgttgt tgacgcgatc gagctcgtct ttgcgcagcc cgtagaggtc
     3421 gcacagtgcc ttcagcatct ggtggaatgc gacctgcgag cgcgtctcgt agaggccgag
     3481 cagcgtgacg agcgcggcgc tctcgtcggt atcgacgtag tcgatcacga ctgcgtccgg
     3541 atccgtcgcc gacgcgagct tcatcgacag cgtgccgagt ggcccgaggc cggctgagcc
     3601 tgccagtagc tccgcgaacg agcgctgtgc gagcgccgct tcgacaagcc tcagccgata
     3661 gcgaaccgcc gagctatcgc cgtgcaggcg cgcgatcagc ggacgcagct gcgcatgcca
     3721 cgggcgcgag acgtcgacgc cgatcttcct cgcggcaccg acggcggcgg cgagcaaccg
     3781 cagctgcgcc gcgggcgaca gaccgctcgt cgcggattcg cagtaccaga gctgacacgt
     3841 ctggagcgcc gggccgagca ccgcgaggtc ctgatcgccg agcgtcgcga ctgcggcgtc
     3901 tacgctcgca gccggctcac ccgcttgttg gcgcccggcg cgcaggttgc gcagccgctc
     3961 ggcgagccgc ttgcgatcga gtggcggcgc gagggcggcg agcgctgcgt atggatcggt
     4021 ccacggcgcc tgcgccgaga cgatcgtgag ctgatcgcgt acgcgggcga gcttgccgag
     4081 cagcacgtcg tcgagcaggc cctcacgcac cgcggccgta tcgcacgcgt tgagcacgtg
     4141 caggccatcg accacgagtg cggtcgcctc gtgtgcctgc aagccacccc cgagcacgcg
     4201 gccgagcgca ggcgcgagct cgcggactgc agcaacgagc ggtgcgaaca tcatcatcgg
     4261 tccttcgccg cgtacgtgct ggccggcgag gccatgcgct tccacccacg cgaccgcaag
     4321 cttctgcagc tgcggcgaca aactccagcc gcgtgcacga tccgctttgc gcaggatcgc
     4381 cgccgccgct tcgttgtgca cct
//
LOCUS       DASUFX010000174         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_862000,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000174 DASUFX010000000
VERSION     DASUFX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862000"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFV99_06940"
     CDS             <1..714
                     /locus_tag="VFV99_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059077.1"
                     /translation="VAVQAWFSMDIWYRPAVATWTREPGVLREIQKSQSLRAALRALG
                     SATETSHWLSLLIETVFESPFIFLFPETKEAYKLTVDGVSDMGQLSALVAPALREPLA
                     RIGITEQPDEEVLEVMRGDGPQQGEGTYSCGFHCYAVEATDPGSGMPRDDVFMWSAPG
                     GTGTHSLPPDFLPGTLTEVDGARVVLVVGPQSPGMRFVRVMPATRTFDGLMARVSPAT
                     KLSADDTRRWFDAAKQRAV"
     gene            751..1641
                     /locus_tag="VFV99_06945"
     CDS             751..1641
                     /locus_tag="VFV99_06945"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5059078.1"
                     /translation="MKVAILLNARAGGFTQVECEQRTQEILAACRAADVQATAHLCDP
                     ARLSVTARQIARDTDVDAVIAAGGDGTVSAVAAGLLGTNVALGVIPLGTLNHFAKDLG
                     IKDVETAIAAIASGATKRIDVGEVNGRVFINNSSIGLYPELVVERETERRRSGRSRWL
                     AMARAALRTLLRFPLLHVAIALAGSVFSTHTPFVLVGNNEYALSVPDVGSRKRLDAGR
                     LSIYTLRSTSRWRMFAMMVRSLLRRRDPELERISVTRADIVTNKRTLRVALDGEVTRM
                     APPLSYRSRPGALVVLGGNA"
     gene            1638..2456
                     /locus_tag="VFV99_06950"
     CDS             1638..2456
                     /locus_tag="VFV99_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5059079.1"
                     /translation="MKLVIHISDPHFGQADPVVVEAMLDEINALRPALVTISGDLTQR
                     ARRAQFMAAREWLDRLIDPYLVVPGNHDIPLYDVMRRFTSPRERYMHFISSDLAPSFI
                     DDEIAVVGIDTTKTLTFKHGRITREQAERVAALVAPHTYHWRILVMHHPFEHASGSED
                     VMPLLEAAGVDLVLTGHMHKTHFDEPAERNDAHTLVKVHAGTCMSTRLRGEPNGYNQL
                     HLDGDHIRIVHREWRDMRFVDGAQKTYRRSERGGERIVKEPPARLRVPQLHLVR"
     gene            complement(2453..2626)
                     /locus_tag="VFV99_06955"
     CDS             complement(2453..2626)
                     /locus_tag="VFV99_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059080.1"
                     /translation="MKKLLSFVLVMAIAAPLAGCIVRTGPRTSSRGSYARSCPPAHHW
                     EGGACVHNGRGRR"
     gene            complement(2694..4394)
                     /locus_tag="VFV99_06960"
     CDS             complement(2694..4394)
                     /locus_tag="VFV99_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5059081.1"
                     /translation="MRPIFVRPLLGLLLASTSLTACLSDENMGDEDLGDIGDGKSDSF
                     GIVDRSTYVNAGKTRSYTFSANAAFRLTITQPADVDQMLDVSITKPDGSKIDVGTGAE
                     PGAVHDPADSGGNGTYTLTIKNTGTKRASLILNVRPLGGFGELPNPNASVFPDAMWQP
                     PALGSWPASYVIFNNPGCGHTCTSAQSTEMAPRSVMVKMLVAAIHDVKQGGTIRVSNF
                     NISSSSSTKPVVDALLWAMQNKAATVKVVMDEAQNNATSRTTLLSQQGAQVRFLDGIH
                     YTNSVGAAVGIMHSKIVVVDDQIVFTGSNNFSSTGFIVNEENSVVLRAPGYSSRIAAF
                     TCDIDKMFEIGVAAGQPQKTDAERRTAILALDQCNTDDVWFPPTGTLATGDSVAYGKV
                     ASAIYNSKKSLSIAPDMLAHPALVTSIVSRAKKAKTAGQPYSVKLVLDASDEALGNPA
                     FGDCLSVAAQKYGLDLQVRYWRGTPEIYQLMHHKFMIVDQDDPAGAALYNGSANYSAK
                     ALKWSFENVTRYKGSELRQVVDSFTARFTKLFADAKDKAGMAADGHAVPNCPLATNSL
                     "
     gene            4667..>4919
                     /locus_tag="VFV99_06965"
     CDS             4667..>4919
                     /locus_tag="VFV99_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059082.1"
                     /translation="MRILPLLLLATAPLATIACVAEDDGEDLGSVIDGKGDGALIDVS
                     ITVPKKSTSGAVGVRNYTVHSTSDFDVSLDYTGDQAAKLT"
BASE COUNT          823 a         1614 c         1594 g          888 t
ORIGIN      
        1 gttgcagtgc aggcctggtt ctcgatggat atctggtacc ggcccgccgt ggcgacgtgg
       61 acgcgcgagc cgggcgtcct ccgcgaaatc cagaagtcgc aatcactccg cgccgcgttg
      121 cgcgcactcg gcagcgccac cgagacgtcg cactggctct cgctcctgat cgagaccgtc
      181 ttcgagtcgc cgttcatatt tctgtttccc gagacgaagg aggcgtacaa gctcacggtc
      241 gacggcgtgt ccgacatggg ccagctctcc gcgctcgtcg cgcccgcgtt gcgcgagccg
      301 ctagcgcgca tcggcatcac cgagcagccc gacgaggagg tgctcgaggt catgcgaggc
      361 gatggcccac agcaaggcga gggcacgtac tcgtgcggct ttcattgcta cgcggtcgaa
      421 gcaacggatc cgggcagcgg catgccgcgc gacgacgtct tcatgtggag cgcgcccggc
      481 ggcacgggca cacacagcct gccgccggac ttcttgcccg gcacgctgac ggaggtcgac
      541 ggtgcgcgcg tggtgctcgt cgtcgggccg caatcacccg gcatgcgctt cgtgcgcgtg
      601 atgccggcga cgcgcacgtt cgacggcttg atggcgcgcg tatcgccagc tacgaagttg
      661 tcggccgatg acacgcgacg ctggttcgat gcagcgaaac agcgcgctgt ctaacgaacg
      721 aggcatatcg cttgctctgt cgtcacatag atgaaggtcg ccatccttct caatgcgagg
      781 gcagggggct ttacgcaagt agaatgcgag cagcgcacgc aggaaatcct cgcagcgtgt
      841 cgcgcagctg acgttcaggc gactgcgcac ctgtgcgacc ccgcgcggct gtccgtcacc
      901 gcgcgccaga tcgcacgcga cacagatgtc gatgctgtaa tcgcggccgg cggtgacggc
      961 acggtgagcg ccgtcgccgc gggactcctc gggaccaacg tcgcgctcgg agtgattccg
     1021 ctcggcacac tgaatcactt tgcgaaggat ctcggcatca aggatgtcga gacggcgatc
     1081 gcggcaatcg cgagcggcgc gacgaaacgc atcgatgtgg gcgaggtgaa tgggcgcgtg
     1141 ttcatcaaca actcctcgat cggtttgtat ccggagctcg tggtcgagcg cgagaccgag
     1201 cgccggcgca gcggccgcag caggtggctc gcgatggccc gcgccgcgct gcgcacgctg
     1261 ctgaggtttc cgctgctcca cgtcgcgatc gcgctggccg gctccgtgtt ctcgacgcac
     1321 acgccgttcg tgctcgttgg caacaacgag tacgcgctga gcgtgcccga cgtcggcagc
     1381 cggaagcggc tcgacgccgg tcgcctttcc atctacacgc tgcgctcgac gagccggtgg
     1441 aggatgttcg cgatgatggt gcgctcgctg ctgcgccggc gcgaccccga gctcgaaagg
     1501 atcagcgtca cacgcgcgga catcgtcacg aacaagcgca cgttacgtgt cgcgctcgac
     1561 ggcgaggtca cgcgcatggc cccaccgctg tcgtatcgca gtcgacctgg tgccttggtc
     1621 gttctcggag gcaatgcatg aaattggtca ttcacatcag cgatccgcat ttcggccagg
     1681 ccgacccggt cgtcgtcgag gcgatgctcg acgagatcaa cgcgcttagg cccgcgctgg
     1741 taacgatctc cggcgacctg acccagcgtg cgcgtcgcgc tcagttcatg gctgcccgcg
     1801 agtggctcga tcgactgatc gatccgtacc tggtcgtgcc cgggaatcac gacattccgc
     1861 tgtacgacgt gatgcggcgg ttcacgtcgc cgcgcgagcg ctacatgcac ttcatctcgt
     1921 ccgatctagc gccgagcttc atcgatgacg agatcgccgt cgtcggtatc gacacgacga
     1981 agacgctcac gttcaaacac ggccgcatca cgcgcgagca ggccgaacgc gtcgccgcgc
     2041 tggtcgcgcc ccacacgtat cactggcgga tcctcgtcat gcatcatccg ttcgagcatg
     2101 cgtccggctc ggaggacgtc atgccgttgc tcgaggctgc gggcgtcgac ctcgtgttga
     2161 ccggtcacat gcacaagacg cacttcgacg aaccggccga gcgcaacgac gcgcacacgc
     2221 tggtcaaggt gcacgccggc acctgcatgt cgacgcggct gcgtggcgag ccgaacggct
     2281 ataaccagct gcacctcgac ggtgaccaca tccgcatcgt ccatcgcgag tggcgcgaca
     2341 tgcggttcgt cgacggtgcc cagaagacct atcgacggtc ggagcgcggc ggcgagcgca
     2401 tcgtcaagga gccgccggct cggcttcgtg ttccgcagct acatctcgtg cgctagcgac
     2461 gtccgcgacc gttgtgcacg caggcgccgc cctcccaatg gtgggcaggc gggcacgacc
     2521 gcgcgtacga gccgcggctg ctggtccgcg gacccgtgcg gacgatgcag cccgcgagcg
     2581 gagctgcgat agccatcacg agcacgaatg acagtagttt cttcatccca cgatcgtagt
     2641 ccgcgatcgt ggtgcgatgc cacccgcacc cctaccacct cgagacgctg tgattacagc
     2701 gaattcgtag cgagcgggca attcggcaca gcatgcccgt cggccgccat acccgccttg
     2761 tccttcgcgt cggcgaatag cttcgtgaag cgcgcggtga acgagtcgac gacctgacgg
     2821 agctcgctgc ccttgtagcg cgtgacgttc tcgaagctcc acttcagcgc cttcgccgag
     2881 tagttcgccg agccgttgta aagcgcggcg ccggccggat cgtcctggtc gacgatcatg
     2941 aacttgtggt gcatgagctg atagatctcc ggcgtgccac gccagtagcg gacctggagg
     3001 tcgaggccat acttctgcgc cgcgaccgag aggcaatcgc cgaatgccgg attgccgagt
     3061 gcttcgtcgc tcgcatcgag cacgagcttg accgagtaag gctggcccgc ggtcttcgcc
     3121 ttcttcgcgc gcgacacgat cgaggtcacc agagccggat gcgcgagcat gtcgggcgcg
     3181 atcgagagcg acttcttcga gttgtagatc gcgcttgcga ccttgccgta cgcgaccgag
     3241 tcgcccgtcg ccagcgtgcc ggtcggcggg aaccagacgt cgtcggtgtt gcactggtcg
     3301 agcgcgagaa tcgcggtgcg gcgctcggcg tcggtcttct gcggctgacc cgcggcgacg
     3361 ccgatctcga acattttgtc gatgtcgcac gtgaacgccg cgatgcgcga gctgtagcca
     3421 ggagcgcgca gcaccacgct gttctcttcg ttgacgatga agccggtgct cgagaagttg
     3481 ttcgagccgg tgaacacgat ctgatcgtcg accacgacga tcttcgagtg catgatgccg
     3541 actgcggcgc cgaccgagtt cgtgtaatgg atgccgtcga ggaagcgcac ctgcgcgccc
     3601 tgttgcgaca gcagtgtcgt gcggctcgtc gcgttgttct gcgcctcatc catcacgacc
     3661 ttcacggtcg cggccttgtt ctgcatcgcc cacaagagcg cgtcgacgac cggcttcgtg
     3721 ctcgacgagc tcgagatgtt gaagttcgac acccggatcg tgccgccttg cttcacgtcg
     3781 tggatcgccg cgaccagcat cttcaccatc acgctacgcg gcgccatctc tgtcgactgc
     3841 gccgacgtgc acgtgtggcc gcagcccgga ttgttgaaga tgacgtacga cgccggccag
     3901 ctgccgagcg caggcggctg ccacattgca tccgggaaca cgctcgcgtt cgggttcggc
     3961 aactcgccga agcccccgag cgggcgcacg ttcaggatca agctcgcgcg cttggtgccg
     4021 gtgttcttga tcgtcagcgt gtaggtaccg ttgccgccgc tgtccgccgg atcgtggacc
     4081 gcgccaggct cggcgccggt gccgacatcg atcttcgaac cgtcgggctt cgtgatcgag
     4141 acatcgagca tctgatcgac atcagctggc tgcgtgatcg tcagccggaa cgccgcattc
     4201 gcggagaacg tgtagctgcg cgtcttgccc gcgttgacgt aggtcgacct gtcgacgatc
     4261 ccgaacgagt cgctcttgcc atcgccgatg tcgcccaggt cttcgtcgcc catgttctcg
     4321 tccgagagac acgcggtgag cgaggtcgaa gcgaggagca aaccgaggag cggacgtacg
     4381 aaaatcggac gcatgttggc gccgtcggac tgcaacgaag gtgccgctgg ccggcattcg
     4441 gacagtcgtc ccagttccgt tacttcactc gcctttgtta cggattcgtt agcgtttccc
     4501 ggccaatcga taagccactg actggctcct gtggccgccg gccggacgcg cagccggccg
     4561 ttcgcacgaa gtgcagggcg aggaatgtcc gcaggttgtc tgccgtcgtg cgcacgattg
     4621 tccgcacgta ccgtccgggc acgtcggttg ctgaatgcgc cagccgatgc ggattctgcc
     4681 ccttctgctc ctggcaacgg ccccgctagc cacgatcgct tgcgtcgccg aagacgatgg
     4741 cgaggacctc ggctcggtca tcgatggcaa aggtgatggc gcactgatcg acgtctcgat
     4801 caccgtgccg aagaagtcga cctccggcgc cgtcggcgtc cgcaattaca cggtgcactc
     4861 gacgagcgac ttcgatgtct cgctcgacta cacgggcgat caggccgcca agctcacgg
//
LOCUS       DASUFX010000175         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1557067, whole genome shotgun sequence.
ACCESSION   DASUFX010000175 DASUFX010000000
VERSION     DASUFX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557067"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..>2109)
                     /locus_tag="VFV99_06970"
     CDS             complement(277..>2109)
                     /locus_tag="VFV99_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5059083.1"
                     /translation="MVTANRIEQPLSRIGNSVTLIEPEEVRASQKLTVADLLATTPGV
                     TVSRNGGLGGATQLRIRGAESDQTVVLIDGVKLNDPASAGGGFDFSNLLTGDYMRIEV
                     LRGPQSTLWGSQAIGGVVNVVTTIPEGPLTAAYSAEGGERGSADFQARAEAGGDRFAW
                     RVAAKYLTTDGVSALDDDLGGKEEDGYRNVGANVRGTVKINDAVSAELRSTYSHGRAE
                     FDGFPAPAFALSDTRDYAVTDELVAYAGLNASTFGGKLHNRVGFAYTDTDRQNIDPDS
                     PVPTTFDATGRNERWEYQGTLTLTETLGGVFGIESERSELNTRSADAFNPNPATLAHD
                     ARLDSVYTQLSYSPIDVLTLTAGLRYDDHDTFGSESTGGASLAWAATPTTLVRASYGE
                     GFKAPTLFQLFSDFGNEALAPEQADAWDVAVEQHLLNDALMLSATYFGRDTDDMIDFV
                     SCFPATDPRCTSRPFGFYDNVAKTEAEGIELGLVAHIGEHLRIDANYTDMDTTNRTPG
                     ANFGRELPRRPGQVFNGEVTYAWPFGLTTTVAVLDVSRNFDDPSNSVVVDGYTVVDFR
                     ASYALRDDLELFGRIENAFDEEYETIARYGTPGRGVFVGVRQTF"
BASE COUNT          354 a          690 c          698 g          367 t
ORIGIN      
        1 gcgcattcag ttcctgcagt tgcaacgacg cgcgacgggt ccgctactcc gaacgcctcg
       61 gccgcgatgg cttccaggcg agccagctct gtcggctccg gcaaccgatt gcgcgaacgc
      121 agcgacgcgc gcggcgcggg atacgaacgc ggattgatac ccgtcgaaag atcgatccac
      181 gcctgctgga cgtccggaaa caacgagcgc gcagcgccga gccgcccgcc atgatgggcg
      241 aagcggcccg ctgcgctttg cgtctcgctg tgcacgtcag aatgtctgac gcactccaac
      301 gaacactcca cgcccgggcg tgccgtagcg tgcgatggtc tcgtactcct cgtcgaacgc
      361 gttctcgatc cggccgaaca gctccaggtc gtcgcgcagg gcgtacgagg cgcggaaatc
      421 gacgaccgtg taaccgtcga ccacgacgct gttcgagggg tcatcgaagt tgcggctgac
      481 gtcgagcact gcaaccgtcg tcgtcaggcc gaacggccac gcgtaggtga cctcgccatt
      541 gaagacctgc cccggacgac gcggcagctc gcggccgaag ttcgcaccgg gcgtgcgatt
      601 ggtcgtatcc atgtccgtgt agttcgcgtc gatgcgcagg tgctcgccga tgtgggcgac
      661 gagcccgagc tcgatgcctt cggcctcggt cttcgcgacg ttgtcgtaga agccgaacgg
      721 ccgcgaggtg cagcgcggat cggttgccgg gaagcatgag acgaaatcga tcatgtcgtc
      781 ggtgtcgcgg ccgaagtatg tcgccgacag catcagcgca tcgttcagaa gatgctgctc
      841 gacagccacg tcccacgcat cggcttgctc tggcgcaagc gcctcgttgc cgaagtcgct
      901 gaacagctgg aacagcgtcg gagctttgaa cccctcgccg tagctcgcac gcaccagcgt
      961 cgtcggcgtc gcggcccacg cgagcgacgc gccgcctgtg ctctcgctac cgaacgtgtc
     1021 gtgatcgtcg taacgcaagc cggcggtcag cgtgagcacg tcgatcggcg aatacgacaa
     1081 ctgggtgtaa acgctgtcga gtcgcgcatc gtgcgccagc gtcgccgggt tcggattgaa
     1141 cgcatccgcg gagcgcgtgt tgagctccga gcgctcggac tcgatgccga acacgccgcc
     1201 gagcgtctcg gtgagcgtca gggtgccctg atattcccag cgctcgttgc gaccggtggc
     1261 gtcgaaggtc gtcggcaccg gcgagtcggg atcgatgttc tggcgatcgg tgtccgtgta
     1321 tgcgaagccg acgcgattgt gcagcttgcc gccgaaggtt gacgcgttga gacctgcata
     1381 cgcgaccagc tcgtcggtga cggcgtagtc gcgcgtgtcg gacagtgcga atgccggcgc
     1441 ggggaagccg tcgaattcgg cacggccgtg gctgtaggtc gagcgcagct cggccgaaac
     1501 cgcgtcgttg atcttcacag ttccgcgcac gtttgcgccg acgttgcgat agccgtcctc
     1561 ttccttgccg ccgagatcgt cgtcgagcgc ggagacgcca tccgtcgtca aatatttcgc
     1621 ggccacgcgc cacgcgaaac ggtcaccgcc cgcttcggcg cgcgcttgga agtcggccga
     1681 gccgcgctcg ccgccttcgg ccgaatacgc tgctgtcagc gggccctcgg gaatcgtggt
     1741 gacgacgttc accacaccgc cgatcgcctg gctgccccac agcgtcgact gcggaccgcg
     1801 cagcacttcg atgcgcatgt agtcgccagt caacaggttc gaaaaatcga aaccgccgcc
     1861 ggccgatgcc gggtcgttga gcttcacgcc gtcgatcagc acgacggtct gatccgactc
     1921 cgcgccgcga atgcgcaact gcgtggcgcc gccaagaccg ccattgcggc ttacggtgac
     1981 gcctggcgtg gtcgcgagca gatcggcaac ggtgagcttc tggctggcgc gaacctcttc
     2041 gggctcgatc aacgtgaccg agttgccgat gcgcgagagc ggctgctcga tgcgattggc
     2101 tgtgaccat
//
LOCUS       DASUFX010000176         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_862125,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000176 DASUFX010000000
VERSION     DASUFX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862125"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFV99_06975"
     CDS             complement(<1..334)
                     /locus_tag="VFV99_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059084.1"
                     /translation="MQRCFFVVIAAALLAPRIGAANSTPELLYFNQPWTDTWRTYYND
                     DWSYVPGVVGFLGQDLTTTPGEDPQLLVGESTLPNDVDLMAAQTAPNSLGTGGVASFY
                     LTDPVVALQ"
     gene            complement(412..2358)
                     /locus_tag="VFV99_06980"
     CDS             complement(412..2358)
                     /locus_tag="VFV99_06980"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5059085.1"
                     /translation="MASSRSVVVVAVFVLASLAGCTGGNDTAPDAGMSGPPAGWTCDP
                     RGYGDGVVCHCNCGVPDPDCASGDHIVSGCVNNEVCTPSGTCTNCGNGQVDSGEACDA
                     ALAATAECAPMGYQPGQVPCNSSCQWAYDQCAPLVTCGNGQLDPVELCDGSQIQSGLD
                     CTDYNRTSGTLKCGSSCQIDSSGCYTCGDGKVEGPEGCDDSGTTNGNGCSSSCAPESG
                     WSCSGSPSFCTPVCGDGMRVGSEACDDGNANSNDGCSASCQVEQDCSCSGSPSVCSCA
                     VVQLVTTTTERIDTGSLALDGNGQPHATWYYGINYTDPVTNHSMAHAYAMYASRPSTS
                     WTTSLLEQWDQIQAGETPESFVLAYDGGALHQYYHRYYTTGGTFAEATKSGSNWSMAY
                     GTQYYNYDVIRGGGNWHALVAGSGFGDYRYYVGSPSGWTTNEPLVNMNTQYPMRLAYA
                     SNGDVYLTTITPASGHTSYNLKLSKRVGPSNWQTMYDVATTGTCVYPVSHEPLALANG
                     EVITFEDGFGTSNQRWLKAHRWNGSSWVVENVADLSWLPYTSCMSSSASWSTQRMVTA
                     ADQLGRPHILFASMPQTNSLTFEDHYRDSTGWHVRTFPMSKGTPLDMIIDAQGTTHIL
                     AMAPSSTPSTTRIVYIRISASAWN"
     gene            2494..2760
                     /locus_tag="VFV99_06985"
     CDS             2494..2760
                     /locus_tag="VFV99_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5059086.1"
                     /translation="MLWTILIGFVVGVLARAIKPGEQAMGWILTTFIGVGGAVLATFI
                     GQGLGWYAYGARAGFLASIGGAILLLVIYEAIKPRLAKKSEQPE"
     gene            2844..4301
                     /locus_tag="VFV99_06990"
     CDS             2844..4301
                     /locus_tag="VFV99_06990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059087.1"
                     /translation="MAVPSEPASTIAWNCPTCGRSYPAEFAVCPLDATPQGELGKPGD
                     PQIGVVLARTYKIVRVLGAGGMARLYEAEHLRIGARFAVKIIHDELAREPSLLARFER
                     EAHAAGRIRSDHVVRLVDVLRTQDERPCIITELLEGEDLQERLDRVGKLSVAEAIPIV
                     RQICRGVAAAHAVGIVHRDLKPSNIFVCPREGEPLVKVFDFGVAKMEDDEKLTRTGAV
                     MGTAAYMAPEQARHAGNVGPLADVYSIGAILYHMLAGQPPYGSLPAVSRFALLLNEEP
                     ARPRSIEPSIPEGVEAVIQHAMARDLASRIPSALELERQLAVFDAPSAAPTPIAVQVS
                     EATAQGIALRARIARPTAVMVAAASSVAAGAWLAGFLGSLLEPMRRGERTLLAILALA
                     AVIGVATLHVRALRAAWASTPAVGRYIAPFARALIGGVMTFGALELIRLGWFSIAHSR
                     LFGVAIQLVGAGVAAVLGLGWRRWKLHTRLGRGMG"
     gene            4314..5285
                     /locus_tag="VFV99_06995"
     CDS             4314..5285
                     /locus_tag="VFV99_06995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059088.1"
                     /translation="MQEGSMIGGRYQLVDLVGEGGLSTVWHGVTRSSEVFARPVAIKV
                     MKRSFTAIGGPYLSMFLEEARIGATLQHANLIQVLDFIVEATAAGPEYCLVLEWVDGI
                     DLKSMLQVHHKLGTPTPWGVIAAVALRVLRGLAAAHERRLPDGTVSPVIHRDVSPPNI
                     LMAANGDVKLADFGMARARDRIAEQTAPGHVKGTLSYLAPELLVGVPATPASDIFSMG
                     CTMWEALAGRRLFYTESDAETFALLKACNVPPLDQIRSDLPVALVETIHEALQADPAN
                     RFPSARAMVHELSEIMRLDDTWGDADVIVGNAVTEARAARQKLAAEA"
     gene            5341..6741
                     /locus_tag="VFV99_07000"
     CDS             5341..6741
                     /locus_tag="VFV99_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059089.1"
                     /translation="MGRGWPVGFVLAVLGCGRIGFDAVGGGVASDANTNPPPDGFGPA
                     ATGCGPLPPTGSVVYVATTGDDNSGDGTMGSPYRTIQRTYDRVAPGTTILVQPGVYAT
                     PLDLTRTVSPERIAIAAVTPYTVTVEDLSQPLSCSGCTGLVLDGLYVHATGSGGLPVL
                     HINGGQDVVIRNCIVDNSGSSATIRLSGDPVNVTVLRNMVFDGNPPLHAAQITDVSIV
                     DNLVVHTRMLNGPLMWLESPLGGTSRIQRNILAGFRGCIDCGMLEMRLDTGTRVESNL
                     LVSSTAQSDGAFKLEGPIDATIQFNTVVGDVPGTAFALTGTEYNATPVSSLILRNNVF
                     ADPTGTLGDFSDGTTTDIPSPVLMSNAYWNNGAAMPPGVNDSVDWTNDPQAVLADPEL
                     PSFAAFTPPVWDAANHQFADGSKTICDEFYRIALSYGMPAAGSPLVGAATGPDRPLDL
                     LGFARTKGAIGALEPQ"
     gene            complement(6790..7635)
                     /locus_tag="VFV99_07005"
     CDS             complement(6790..7635)
                     /locus_tag="VFV99_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059090.1"
                     /translation="MRWAVAAVLLTGCVSIPAFRGSGDDAGADTPDGSIGPLAVRHIA
                     NAYREGGSDGQAFGTYMMTKAGYSIPTAGIVDGDLVLFIANVDNGSNDFWNLPTGFTP
                     ILQRFYGQDGQTYVAGWKIAASEPQTYAGMYKVGVSSSSAATVSLLAVTGYDPQSPIE
                     FAVPTDHQTPTNPADIGSAGLSTTADNSLLIYAAGADWTPENGQNTYELPSGFQLLTF
                     VGDRGDTPPHWDWTCQVIAYKLQATKGASGPLTGTMTGVSFSDGTTPIPGGGWDLLFA
                     IAPHS"
BASE COUNT         1306 a         2547 c         2692 g         1459 t
ORIGIN      
        1 cttgcagtgc aacgaccgga tccgtgaggt agaagctcgc gacaccacca gtgcccagcg
       61 aattcggagc agtctgggcc gccatgaggt cgacatcgtt tggcagcgtg ctctcaccga
      121 cgagtagctg agggtcttca cctggcgtcg tcgtgagatc ttggccgagg aagccgacga
      181 cacccggcac gtagctccag tcgtcgttgt aataagtccg ccacgtatcc gtccacggct
      241 gattgaaata gagcagctcc ggagtcgaat tggcggctcc gatgcgcggc gcgagcagtg
      301 ccgcggcgat cacgacgaag aaacagcgct gcacggcgag ccctcattgt agtcgatcgt
      361 ccgtgacgac cgaatgtgcg cgttcgcccg acagcaacaa agtgctgcgg ctcagttcca
      421 ggcgcttgcg ctgatccgga tatagacgat gcgcgtcgtg ctcggcgtcg agctcggtgc
      481 catcgcgagg atgtgcgtcg tgccctgggc gtcgatgatc atgtcgagcg gtgtcccctt
      541 cgacatcggg aacgttcgga cgtgccagcc ggtcgaatcg cgatagtggt cctcgaacgt
      601 gagcgagttg gtctgcggca tcgacgcgaa caggatgtgg ggtcggccga gctgatcagc
      661 ggcagtgacc atgcgctggg tcgaccacga tgcgctgctc gacatgcagc tcgtgtacgg
      721 cagccacgac aggtcggcga cgttctcgac gacccagctc gagccgttcc agcggtgcgc
      781 cttgagccag cgctggttcg acgtaccaaa gccgtcctcg aacgtgatca cttcgccatt
      841 cgcgagcgcg agcggctcgt gcgagaccgg atagacgcac gtgccggtcg tcgcgacgtc
      901 gtacatcgtc tgccagttcg acgggccgac gcgcttcgag agcttcaggt tgtacgacgt
      961 gtgtcccgac gccggcgtga tcgtcgtcag gtagacgtcg ccgttcgacg cgtacgcgag
     1021 ccgcatcgga tactgcgtgt tcatgttcac gagcggctcg ttcgtcgtcc agccgctggg
     1081 cgagccgacg tagtagcggt aatcgccaaa gccgctgcca gcgacgagcg cgtgccagtt
     1141 cccgccgccg cgaatgacat cgtagttgta gtactgcgtt ccgtacgcca tcgaccaatt
     1201 cgagccgctc ttcgtcgcct cggcgaacgt gccgcccgtg gtgtagtagc ggtggtagta
     1261 ctggtggagc gcaccgccgt cgtacgcgag cacgaagctc tccggtgtct cgccggcctg
     1321 gatctgatcc cactgctcga gcaggctggt cgtccaactc gtgctcgggc ggctcgcgta
     1381 catcgcgtag gcgtgcgcca tcgagtggtt cgtgaccggg tcggtgtagt tgatgccgta
     1441 gtaccaggtc gcgtgcggct ggccattgcc gtcgagcgcg agcgagccgg tgtcgatccg
     1501 ctcggtcgtc gtcgtgacga gctgcaccac ggcgcagctg cacaccgatg gcgaaccgct
     1561 gcacgagcag tcctgttcga cctgacagct cgcgctgcag ccgtcgttgc tgttcgcgtt
     1621 gccgtcgtcg cacgcttcgc tgccgacgcg catgccgtcg ccgcagaccg gcgtgcagaa
     1681 cgacggactg ccgctgcacg accagccgct ctcgggcgca cacgagctcg agcatccgtt
     1741 gccatttgtc gtgccgctgt cgtcgcagcc ttccggccct tcgaccttgc cgtcgccgca
     1801 cgtgtaacag ccgctcgaat cgatctgaca gctcgaacca cacttcagcg tgccgctcgt
     1861 ccggttgtag tcggtgcagt cgaggcccga ttggatctgc gagccatcgc agagctcgac
     1921 gggatcgagc tggccgttgc cgcacgtgac aagcggcgcg cattgatcgt aggcccactg
     1981 gcagctcgaa ttgcacggca cctgacccgg ctgatagccc atcggtgcgc actcggcggt
     2041 ggcagcgagc gcggcgtcgc atgcttcgcc gctgtcgacc tggccgttgc cgcagttcgt
     2101 acacgtgccg ctcggcgtgc agacctcgtt gttcacgcag cccgacacga tgtgatcgcc
     2161 gctcgcgcag tccggatcgg gcacgccgca gttgcaatga cagacgacgc cgtcgccgta
     2221 gccgcgcgga tcgcacgtcc agcccgctgg cggtccgctc atgccagcgt cgggcgctgt
     2281 gtcgttaccg ccggtgcagc cggcgagcga tgcgaggacg aatacggcta cgacaaccac
     2341 agaacgcgag gacgccatgc ccccacaacg tgcgaggcgc cagaactggg aactatttca
     2401 ggcgactccg atgcccattg acgggggccc catgcgtaca cacaacttag gacatcgcgc
     2461 cgactacctg gcgcagactg ttcggaggca gaaatgcttt ggaccatcct gatcggtttt
     2521 gtcgtgggtg tgctcgcgcg tgcgatcaag ccgggcgagc aggcgatggg ctggatcttg
     2581 acgacgttca tcggcgtcgg tggtgcggtg ctggccacgt ttatcggcca gggactcggt
     2641 tggtatgcgt atggcgctcg cgcagggttt ctcgcctcga tcggcggcgc gatcctactc
     2701 ttggtgatct acgaggccat caagccgcgg ctcgccaaga aatccgaaca gcctgagtga
     2761 acctcgtgag ctaacttccg tcgacaggtg ggaatgatcc ctgtcgcagc cggatcggcc
     2821 caaatccgga ctagtctgct gggatggctg tgccgtcgga acccgcgagc acgatcgcgt
     2881 ggaactgccc gacctgcggc cgttcctacc cggcggagtt cgcggtgtgt ccgctcgatg
     2941 cgacgccgca gggcgagctc ggaaagccgg gcgacccgca gatcggcgtc gtgttggcgc
     3001 gtacgtacaa gatcgtacgc gtactcggtg cgggcggcat ggcgcggctc tacgaagccg
     3061 agcacctccg catcggcgct cgtttcgcgg tcaagatcat ccacgacgag ctcgcgcgtg
     3121 agccgagctt gctcgctcgc ttcgaacgcg aggcgcacgc ggctggccgc atcagatcgg
     3181 atcacgtggt ccggttggtc gatgtcttgc gcacgcaaga cgagcggccg tgcatcatta
     3241 ccgagctcct cgaaggcgaa gacctgcaag agcggctcga tcgcgtcggc aagctgagcg
     3301 tcgccgaagc gattccgatc gtgcgtcaga tttgccgcgg tgttgcggca gcgcatgcag
     3361 tcggcatcgt gcaccgcgac ctcaagccgt cgaacatctt cgtgtgtccg cgcgaaggtg
     3421 agccactcgt gaaggtgttc gatttcggcg tcgcgaagat ggaggacgac gagaagctca
     3481 cgcgcactgg cgcggtgatg gggacggccg cgtatatggc tcccgagcag gcgcgccatg
     3541 ctgggaacgt ggggccgctc gccgacgtct actcgatcgg cgcgatcctc tatcacatgc
     3601 tcgccgggca gccgccgtac ggaagcttgc cagcggtgag ccgcttcgcg ctgcttctga
     3661 atgaagagcc ggcgcgtccg cgctcgatcg agccatcgat tccggagggt gtcgaagctg
     3721 tgatccagca cgcgatggcg cgtgatctgg cgtcgcgcat tccgtccgcg ctcgagctcg
     3781 agcgtcagct cgcggtgttc gatgcaccgt cggcggctcc gacgccgatt gcggtgcagg
     3841 tcagtgaagc gaccgcacaa ggcatcgcgc tgcgcgcacg catcgcacgc ccgacggcgg
     3901 tgatggtcgc tgctgcttcg agcgttgccg cgggggcgtg gctcgctgga ttccttggat
     3961 cgctgctcga gccgatgcga cgaggtgagc gcacgttgct cgcgatcctc gcgctcgcgg
     4021 cggtgatcgg cgtggcgact ctgcacgtgc gcgcgttgcg tgcagcgtgg gcgagcacgc
     4081 ctgcggtcgg tcgctacatc gcgccgttcg cgcgtgcgtt gatcggcggc gtgatgacgt
     4141 tcggtgctct cgagctgatc cggctcggtt ggttctcgat cgcacactcg aggttgttcg
     4201 gagttgcgat tcagctcgtc ggtgcaggcg tcgcggcggt gctcgggctc gggtggcgaa
     4261 gatggaagct ccacacgcga ctcggtcgcg ggatgggcta gcatcggggc ggtatgcaag
     4321 aaggctcgat gatcgggggc cgctatcaac tcgtcgactt ggtcggcgag ggcggcctct
     4381 cgacggtgtg gcacggcgtc acccggtcga gcgaggtgtt tgcgcgtccc gtcgcgatca
     4441 aggtgatgaa gcgctcgttc acggccatcg gcgggccgta cctgtcgatg tttctcgaag
     4501 aagcgcgcat cggcgcgacg ctgcagcacg caaaccttat ccaggtgctc gacttcatcg
     4561 tcgaagcgac tgccgccggg ccggagtatt gcctcgttct cgagtgggtc gatggcatcg
     4621 atctgaagtc gatgctgcaa gtgcatcaca agctcggcac gccgacgccg tggggcgtga
     4681 tcgcggcggt cgcgctgcgc gtgttgcgtg ggcttgcagc ggcgcacgag cgacggctgc
     4741 ctgacggcac ggtatcgccg gtgattcacc gcgacgtgtc accgccgaac atcctgatgg
     4801 cggccaatgg cgacgtgaag ctcgccgact tcggtatggc gcgcgcacgc gatcgcatcg
     4861 cggagcaaac cgcgccgggc cacgtcaaag gcacgctgtc gtacctggcg cccgagctgc
     4921 tcgtcggtgt accggcgacg ccggcgagcg atattttctc gatgggctgc acgatgtggg
     4981 aagcacttgc ggggcggcgg ctcttctaca cggaatctga cgccgagacg tttgcgctct
     5041 tgaaggcgtg caacgtgccg ccgctcgatc agattcgcag cgacttgccc gtcgcacttg
     5101 tcgaaacgat ccatgaagca ctccaggcgg atccagcgaa ccggtttccg tctgcgcgcg
     5161 cgatggtgca cgagctcagc gagatcatgc gactcgacga tacgtggggc gacgccgacg
     5221 tgatcgtcgg caatgcggtg accgaggcac gcgcggcacg tcagaagctc gccgccgaag
     5281 cctgagcagc agccacggaa tgctggaatt gcaaccagct ctcgcccaca atggttcggc
     5341 atggggcgtg ggtggcctgt cgggttcgtt ctggccgtgc tcggctgcgg acggatcggc
     5401 ttcgacgccg tcggcggtgg cgtggccagt gacgctaata cgaatccgcc gccggatggt
     5461 ttcggacctg ccgcgacagg ctgtggaccg ctgcctccga cgggctcggt cgtgtacgtc
     5521 gcgacgaccg gcgatgacaa tagcggcgat gggacgatgg gctcgcccta tcgcacgatc
     5581 cagaggacct acgatcgcgt cgcgccgggc acgacgatcc tcgtgcagcc gggcgtctac
     5641 gcgacgccgc tcgatctgac gcgcacggtg tcgccggagc ggatcgcgat cgctgcggtg
     5701 acgccgtata ccgtgaccgt ggaggacctg tcgcagccgc tgtcatgctc gggctgcacc
     5761 ggtctcgtgc tcgatgggct ctacgtgcac gcgaccgggt cgggcggctt gccggtcctg
     5821 catatcaacg gggggcagga cgtcgtgatc cgcaactgca tcgtcgacaa cagcggctcg
     5881 agtgcgacga ttcggctatc gggtgatccg gtgaacgtga ctgtgctccg caacatggtc
     5941 ttcgacggca acccgccgct ccatgctgcg cagatcaccg acgtgtcgat cgtcgacaac
     6001 ctggtcgtgc acacgcgcat gctcaacggg ccgctgatgt ggcttgagtc cccgctcggt
     6061 gggacgtcgc ggatccagcg aaacatcctc gctgggtttc gtggctgcat tgactgcggc
     6121 atgctcgaga tgcgcctcga caccggaacg cgcgtcgaga gcaacctgct cgtgagcagc
     6181 accgcgcaat cggatggtgc gttcaagctc gaggggccca tcgacgcgac catccagttc
     6241 aataccgtgg tcggtgatgt tccgggcacc gcgttcgcgc tcaccggcac cgagtacaac
     6301 gccacgccgg tctcgagcct catactcagg aacaacgtgt tcgccgatcc gacgggcacg
     6361 ctcggcgact tctcggacgg cactacgacc gacatcccga gcccggttct gatgagcaac
     6421 gcatactgga acaacggcgc ggcgatgccg cccggcgtca acgactccgt cgattggacg
     6481 aacgacccgc aggccgtgct cgccgaccca gagctgccga gcttcgcagc gttcacgccg
     6541 ccggtgtggg acgcggcgaa ccaccagttt gccgacgggt cgaagacgat ctgcgacgag
     6601 ttttatcgca tcgcactttc gtacggcatg ccggccgcgg gcagtccgct tgtcggtgcg
     6661 gcgacgggtc ctgatcgacc gctcgacttg cttggctttg cgcgcacgaa aggtgcgatc
     6721 ggcgcgctcg agccgcagtg acgccatcgc tatgcgctcg tgcacgcgtt gcgacccgga
     6781 ccgtgaccgc tacgaatgcg gtgcgatcgc gaacaagagg tcccagccgc cgccgggaat
     6841 cggcgtcgtg ccgtcgctga acgaaacacc cgtcatcgtg ccggtgagcg ggccgctcgc
     6901 acccttcgtc gcttgcagct tgtacgcgat gacctggcac gtccaatccc agtgcggcgg
     6961 cgtatcgccg cgatcgccga cgaacgtgag cagctgaaag cccgagggca gctcgtaggt
     7021 gttctggccg ttctccggtg tccagtcggc accggccgca tagatgagca agctgttgtc
     7081 ggcggttgtc gacaggccgg ccgagccgat atcggctggg ttcgtcggcg tctgatgatc
     7141 ggtcggcacc gcaaactcga tcggactttg cggatcgtaa ccggtgaccg cgagcagcga
     7201 caccgtcgcc gcggagctcg acgacacgcc gaccttgtac atgccggcat acgtctgcgg
     7261 ttcgctcgcg gcgatcttcc agccggcgac gtacgtctgg ccatcttggc catagaagcg
     7321 ctgcaggatc ggcgtgaagc ccgtcggcag gttccagaag tcgttagagc cattgtcgac
     7381 attcgcgatg aacaggacca gatcgccatc gacgatgccg gcagtcggaa tcgaatagcc
     7441 ggccttcgtc atcatgtacg tgccgaacgc ctggccgtcg ctgccgccct cgcggtacgc
     7501 gttcgcgatg tggcgcacgg cgagtggacc gatgctgcca tccggcgtat cggcgcccgc
     7561 gtcatcgccg ctgccgcgga acgctggtat cgacacgcat ccggtcagga gtacagcagc
     7621 cacggcccag cgcatgtttc gccgagtata gggcgcagct agccgatttg cgcactcgtg
     7681 attcgggctt attcgccggt cgtgcacgca gtatgcagtg catcaccaca tgcccacgct
     7741 atccaagcac cagcgcgact caggcgaatc ccgtgcggcg aaagacgcaa tgcggtttct
     7801 gagctggtcg agctttattg cggccgtggt cgtcctgctt cttctggcca tcatcgtcat
     7861 gcggatgagc tgacggtcga tcagcggctc gcgagcagcg acatgtgcgc cgggaacgat
     7921 ccagcctcgt cggacggagc cgggatcgtc agcgccgcca acttggtcgc gacgcagtcc
     7981 gcgaacgccg acggtgtggg tgcc
//
LOCUS       DASUFX010000177        11815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1416610, whole genome shotgun sequence.
ACCESSION   DASUFX010000177 DASUFX010000000
VERSION     DASUFX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416610"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFV99_07010"
     CDS             <1..455
                     /locus_tag="VFV99_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5059091.1"
                     /translation="SISGSTSRYRRGGLDAPWTSAETLCEMDGVGIMRRTHLAVIQDE
                     VERTRLFTTLGTNNLKNFWIGLSDRKTEGTMKWVTAEPIGVPQDGEDPPWAAGQPDNN
                     SMQTGGQDCVRMIAPLSTEQGLFDDVECLANFDFVCECDDYPAVLANF"
     gene            complement(452..826)
                     /locus_tag="VFV99_07015"
     CDS             complement(452..826)
                     /locus_tag="VFV99_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059092.1"
                     /translation="MKTDTPGFLDVKLTVVTKDKGPWLDGLAAIGAAESEETNGDWTI
                     RRVPTSAKLGELVIRVLGYVGPPTADVTVGWVGSDRIVSDGTTEIPDVSIAVVPRIAG
                     EPGKDTLMTALKDALREAAKSS"
     gene            1069..3192
                     /gene="pnp"
                     /locus_tag="VFV99_07020"
     CDS             1069..3192
                     /gene="pnp"
                     /locus_tag="VFV99_07020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5059093.1"
                     /translation="MGFVRETAIVGGKEIIIETGKWAKQAGGSVVVRCGDSMVLVTAA
                     GSQQPRDIDFMPLTCEYQEKFYSSGKIPGSFFRREGRSTNAEILVARLMDRPVRPLFP
                     KGWFIDTQIIANVVSFDKENATDVLAMTGASAALTISDLVWEGPLAGVRVGRIDGQFI
                     ANPTFAEREKSEMDIVVAASKDAIMMVEGEMNELQEDVVIDALLFAHQAVQPLIELQE
                     RLRASHGKEKRPFVKPETDATLADRVKELAWDRIAEAMQTKDKKERRNKLSQTHLDTR
                     NALVAEGQPWFGKNKEVDTAFGKLEKKWARGHTLKTRTRIDGRKLDEVRPIMIETNVL
                     PRAHGSALFQRGETQALVAVTLGTKYDEKKLDTLRGDEKRTFYMDYNFPPFSTGEVKP
                     LRGQSRREVGHGFLAERSVESVVPIYEDFPYTIRVVSDILESNGSSSMASGCGGSLAL
                     MDAGVPTKAPVAGIAMGLMKEGDDYAILSDILGDEDHMGDMDFKVTGTRRGICALQMD
                     IKVDGLTRKILEEALEQAKRGRVHILDKMDAAISAPRDEVSIYAPRIVTVQIKPDKVR
                     DIIGPGGKTIRAIQEQTGAQIDINDNGTVSIASADAKSIEQAQALISGLTMEPEVGQF
                     YNGIVKKIVEIGAFVEIMPGTDGLLHISEVAKERIRSVEDVLSEGDEVIVKCIKVDRD
                     GKIRLSRREALDANPGPEEVHNFVI"
     gene            3330..4664
                     /locus_tag="VFV99_07025"
     CDS             3330..4664
                     /locus_tag="VFV99_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX5059094.1"
                     /translation="MRSVLSFMLCVAAIGLASTAAHAQDTWSDPHPGMRRLHRTTADQ
                     NINVLVVDLCASGVSVRATKTGERQRTVSSFGALVGAEAAINGDFFSFETYGTNGPAM
                     SGGAAWGGTDHNYVAPVQFGAYRVALPPHEATGGVEPWAREVVSGHPTLLVAGAPRNN
                     NGDTLCTARHPRTALGLSADKEKLFLAVVDGRATGRIGMTCDELGALLEGLGASDAVN
                     LDGGGSSTMWLAGTGVLNFPSDGAQRVVANHLAINATGTGAAPNCPHPKFAATFVGSE
                     APMEMTSGDEAVVFLEATNDGNASWDITQTRVGTQDPQDRDSAFFKAENWIAPNRPTG
                     ADHTYAPGATGRFTWAMVAPEVEKSTMFDETFQLVHEGVAWFGEKRTMTILVHPRSGP
                     TTDDGDGSGSDDAPSNSGCNAADGSPTWPLVMIVLLAYWRRPRPDRLDRARR"
     gene            4713..5612
                     /locus_tag="VFV99_07030"
     CDS             4713..5612
                     /locus_tag="VFV99_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059095.1"
                     /translation="MGQVASLGSRGLAATLWFVVWGCGRVNFDSRSDANDASDATGQL
                     GPPMVVGELTVGGVDADDPTLTADMLEIYFASSRPGGVGSGDIWRSIRNSVDEPWQPP
                     VVVTELDSASVEESPGITADGLEMYFTSDRGGGNDIWASTRPTRGDMWAAPTVVAELS
                     TGSGDFQPQPSATKLRIVFYRSSGNRDIYEATRTAVGATWQSPAVLANVNSAAGDRSP
                     CLDGSELTMVFSSDRDSATADVQDLYLATRASLTDPFGAAQPLTDLNSQFDDDSAWLS
                     SDGHVLFFSSDRSGTSEIYVTQR"
     gene            5670..6110
                     /gene="dut"
                     /locus_tag="VFV99_07035"
     CDS             5670..6110
                     /gene="dut"
                     /locus_tag="VFV99_07035"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5059096.1"
                     /translation="MTTPRVQIRRLRPDAIVPRYQTADAAGMDLHAALDQPMQVAPGA
                     VAAVPTGLAMAIPRGFEGQVRPRSGLARKHAITVANAPGTIDADYRLEVMVLLINLGR
                     EPYVIQPNDRVAQLVIAPVVQAELVEVAELDETARTGGFGSTGR"
     gene            6118..6642
                     /locus_tag="VFV99_07040"
     CDS             6118..6642
                     /locus_tag="VFV99_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059097.1"
                     /translation="MSARSKKPTSKGQAPPPFTDENKKTVVYDSNKGKKSGGQYAEPD
                     PTVVDGPLGGAKMYEKPAPNAGPTVLEKPRPPVVAVSKKEQSAPIKAISMKTPNDQQA
                     PAPEKTPMLARPKLRAVSEVMPAQTPQNLGNLAQPYDPKEARSRAMREYVIWGCVAVI
                     LASAIALVVWFAAR"
     gene            complement(6639..8270)
                     /locus_tag="VFV99_07045"
     CDS             complement(6639..8270)
                     /locus_tag="VFV99_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059098.1"
                     /translation="MRWVLLGILALGCGKETAKQTDGGTDDACLAVTACEGGDGCCPA
                     GCSPADDNDCSTTCGNGTVEGNETCDPPESCPVACPAPICMAGTLIGSSANCNAACAT
                     TPITACTNGDSCCAPGCDSNNDNDCVPASSPLQDRLVVASITTPAGVIPGISNWRIWG
                     MGSLRIGPVYTVPFADCGTLVGYTTGTTTNPRARVARLNAMDQLVTTYDLGAFTLRGL
                     AAEPDGHFAALLWDQAPTPKSLHVTRFTSNGTAGWTTSLDNALAAPTDFAIGESRLEY
                     GGGKYGAYFHVHGISGFANGHEGDAIHWLAAATGADTLGWDWGCSHSMSELLRFSPAA
                     NLTLPVCVTDCYPGTTGSNFPTDSIGGIYLNHQDQKVMNVDGGCNGSVAGELGSIAPG
                     TAGWKLVFNTHQNAATNGQSSYSSSTMNQDIGFVSIANNKTPGAVVWLTTTTGNEANS
                     SIARWKPDGDATEQYVVGWSSGTTYSLARVSATGNFLEGPTTITTAKWGERDDPFRDH
                     LNGDIVWAWFDSAGSTTLKFARVRSGGTATCTSLP"
     gene            8325..9674
                     /gene="purB"
                     /locus_tag="VFV99_07050"
     CDS             8325..9674
                     /gene="purB"
                     /locus_tag="VFV99_07050"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5059099.1"
                     /translation="MIPRYSRPELAQLWDDKYRFELWLEIELSVCEAMEQRGTVPAGT
                     AAKVRAAAAGKLDPAKILEIEATTRHDVIAFLTHVEQLAGEPARWLHLGMTSSDVLDT
                     CLALQTGRALEKIVQGVMGLANVLRDRAREHAKTPTIGRSHGIHAEPITAGLVFARWH
                     AEVVRALNRIIRARHAIAVGKIAGAVGVYGNLDPEIERQALGSLGLEPETVATQIVAR
                     DRHAEVFCALALLGTAIEQIALGVRHWQRTEVGEAEEGFGAGQKGSSAMPHKKNPILS
                     ENLTGLARLLRSYAQAGLEDVALWHERDISHSSVERVAMPDATILADFMVARATGLVK
                     GLIVHERRMKANLEKTGGLYFSEAVLLALVKTGLPRQEAYVLVQKAALAAIAEAAQMG
                     HVGALPGRFRELLGQDSEVTKRLDAKALDACFDLEHHLRHAAEILDRALGAEGSHGN"
     gene            9664..10653
                     /locus_tag="VFV99_07055"
     CDS             9664..10653
                     /locus_tag="VFV99_07055"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5059100.1"
                     /translation="MATDLDTSLRGQLNQTLTHTPWKTIGGRTLPRYDGKVRDCYIDV
                     ERGERIIVVTDRLSAFDAVVGTIPFKGQVLNQLAQFWFEQTKAIAPNHMLRVPDANVM
                     VARECEPLPVELVMRSYLTGVTSTSIWKAYEAGARTFCGHPLPEGMKKNQRLPKAILT
                     PSTKAAKGDHDISVSKDELLAMGRIKPEIFERAAAIAEKLFAAGQAHAAKQGLILADT
                     KYEMGLSRDGTIVVIDEIHTPDSSRYWYAEDYEQRLANAEEPRSLDKEYVRRWLANEA
                     KWTGDGPPPDMPGEVRVEAARRYIASYEIVTGRTFVPDTREPIARIAAALGAA"
     gene            10712..>11815
                     /locus_tag="VFV99_07060"
     CDS             10712..>11815
                     /locus_tag="VFV99_07060"
                     /inference="COORDINATES: protein motif:HMM:NF037184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059101.1"
                     /translation="MLIVPGRAALSAARKASVLAKIREVSPHVTSLEARWVHMVVAER
                     ELTTDELTQLGHMLSYGPAEPPTRPSRRLETVETVSFVITPRIGTVSPWSSKATDIAH
                     VCGLTAITRIERGTEWTLVGDAIDLAAVARVLSDRMTESVITREQDLAKVVAPAGTPR
                     PLQFIQLGARGSETLRDASTRLGLALADDEIEYLVARYKELGRDPSDVELMMFAQANS
                     EHCRHKIFNAEWYLSGEKQDRSLFQWIKRSTESAPAGVLSAYKDNAAVIEGATAERFF
                     PDPDGVYRGHLEPAHILGKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAG
                     LVGFTVSDLRIPDALEPWERDEWIGSPARIATAL"
BASE COUNT         2123 a         3794 c         4015 g         1883 t
ORIGIN      
        1 tcagcatctc tggctcgacg tcgcgatatc ggcgcggcgg cctcgacgca ccgtggacga
       61 gcgctgaaac gctgtgcgag atggatggcg tcggcatcat gcgtcgcacg catctcgccg
      121 tgattcagga cgaggtcgag aggacgcgcc tgttcacgac gctcggtacg aataacctca
      181 agaacttctg gatcgggctt tcggatcgca agaccgaggg cacgatgaag tgggtcacgg
      241 ccgagccgat cggcgttccg caggacggcg aggatccgcc gtgggcggct gggcagccgg
      301 ataacaactc gatgcagacg ggcggccagg attgcgtgcg gatgatcgcg ccgctgtcca
      361 cggaacaagg cctcttcgac gacgtcgagt gcctcgcaaa cttcgacttc gtgtgcgagt
      421 gcgatgacta tccagcggtg ctagcgaact tctagctgct cttcgcagct tcgcggagcg
      481 cgtctttcag cgcggtcatc agcgtgtctt tgcccggctc accggcgatg cgcgggacca
      541 ccgcgatcga gacatcggga atctcggtag tgccatcgct gacgatgcgg tccgaaccga
      601 cccagcccac ggtgacgtcc gcggtcggcg gaccgacgta gccgaggacc cgaatgacca
      661 gctcgccgag cttggccgac gtcggcacgc gccggatcgt ccaatctcca ttggtctctt
      721 cgctctctgc ggcgccgatg gccgcgaggc cgtcgagcca agggcccttg tccttggtga
      781 cgacggtcag cttgacgtcc aaaaatccgg gagtgtccgt cttcatgggg ggcgcccgag
      841 acgctaacac gtggatctcg ttcgatcagg ccctttgtcg gtcgtcttgg ctttcgggag
      901 ccgctgtgca ataacgcccg cggaaacaac taggtagcgt cgtaggtcag cagggatcgc
      961 gtaggtttga tcgttccgtc gagctctcga acactcgaga gctggataga gggttcaggc
     1021 cgctcgatcg aagcgcccca cgacgtgatt tgaaaaagga cacgttcaat gggattcgtt
     1081 cgtgagaccg ccatcgtcgg cggcaaggaa atcattatcg agacgggcaa gtgggccaag
     1141 caggccggtg gctccgtcgt cgttcgctgc ggcgacagca tggtgctcgt caccgccgcc
     1201 ggttcgcagc agccgcgcga catcgacttc atgccgctga cctgcgagta ccaggagaag
     1261 ttctactcgt cgggcaagat ccccggcagc ttcttccgcc gcgagggtcg cagcacgaac
     1321 gcggagattc tggtcgcgcg tctcatggac cgcccggttc gcccgctgtt cccgaagggc
     1381 tggttcatcg acacgcagat cattgcgaac gtcgtgtcgt tcgataagga gaacgcgacg
     1441 gacgtgctcg cgatgacggg cgcctcggcg gcgctcacca tctcggacct cgtctgggaa
     1501 ggcccgctcg cgggcgtgcg cgtcggccgc atcgacggtc agttcatcgc gaacccgacg
     1561 ttcgcggagc gcgagaagag cgagatggac atcgttgtcg cggccagcaa ggacgcgatc
     1621 atgatggtcg aaggcgagat gaacgagctg caggaggacg tcgtgatcga cgcgctcctg
     1681 ttcgcgcatc aggctgtgca gccgctcatc gagctgcaag agcgcctgcg cgcgtcgcac
     1741 ggcaaggaga agcgtccgtt cgtgaagccc gagaccgacg cgacgctcgc cgaccgcgtg
     1801 aaggagctcg catgggaccg catcgccgag gcgatgcaga ccaaggacaa gaaagagcgc
     1861 cgcaacaagc tgagccagac gcacctcgac acgcgcaacg cgctcgtcgc cgaaggtcag
     1921 ccgtggttcg gcaagaacaa ggaagtcgac accgcgttcg gcaagctcga gaagaagtgg
     1981 gcgcgcggcc acacgctgaa gacgcgtacg cgcatcgacg gccgtaagct cgacgaagtt
     2041 cgcccgatca tgatcgagac gaacgtgctg ccccgcgcgc acggctcggc gctgttccag
     2101 cgtggcgaga cccaagcgct cgtcgcggtg acgctcggca cgaagtacga cgagaagaag
     2161 ctcgataccc tgcgtggtga cgagaagcgc acgttctaca tggactacaa cttcccgccg
     2221 ttctcgacgg gtgaagtgaa gccgctgcgc ggccagtcgc gccgcgaggt cggtcacggc
     2281 ttcctcgccg agcgttcggt cgagagcgtc gtcccgatct acgaggactt cccgtacacg
     2341 atccgcgtcg tcagcgacat cctcgagtcg aacggctcgt cgtcgatggc gtcggggtgc
     2401 ggcggctcgc tcgcgctcat ggatgcgggc gttccgacga aggcgccggt cgcgggcatc
     2461 gcgatgggcc tcatgaagga aggcgatgac tacgccatcc tgtcggacat cctcggcgac
     2521 gaggaccaca tgggcgacat ggacttcaag gtgacgggca cgcgccgtgg catctgcgcg
     2581 ctgcagatgg acatcaaggt cgacggcctg acccgcaaga tcctcgagga agcactcgag
     2641 caagcgaagc gtggccgcgt ccacatcctc gacaagatgg atgcggcgat cagcgcgccg
     2701 cgcgacgagg tctcgatcta cgcgccgcgc atcgtgacgg tgcagatcaa gccggacaag
     2761 gtccgcgaca tcatcggccc cggcggcaag acgatccgcg cgatccagga gcagaccggc
     2821 gcgcagatcg acatcaacga caacggcacg gtgtcgatcg cgtcggccga tgcgaagagc
     2881 atcgagcagg cgcaggcgct catctccggc ctcacgatgg agcccgaggt cggccagttc
     2941 tacaacggca tcgtgaagaa gatcgtcgag atcggcgcgt tcgtcgagat catgccgggc
     3001 accgatggtc tccttcacat cagcgaagtc gcgaaggagc gcattcgctc cgtcgaggac
     3061 gtgctgtcgg aaggcgacga ggtcatcgtc aagtgcatca aggtcgaccg tgacggcaag
     3121 atccgtctgt cgcgccgcga agcgctcgac gcgaacccgg gtcccgaaga agttcacaac
     3181 ttcgtgatct gatcgatcgc ggatcgatca ccatcgcgac cggtgcacac attcggtcgc
     3241 gatgctgcac acgagcgttc gcactggagc acttcaactg cagcaggcag cgcgtgtgag
     3301 cgggagcagc tcttgcagcc tggggccgca tgcgttcggt tctctcgttc atgctgtgcg
     3361 tcgcagcgat cggcctcgca tcgacggctg cgcacgcgca agacacgtgg tcggatccgc
     3421 atcccggcat gcgccggctg catcggacca ccgccgacca gaacatcaac gtgctggtgg
     3481 tcgatctgtg cgcgagcggc gtcagcgtgc gcgcgacgaa gaccggcgaa cggcaacgaa
     3541 ccgtgtcgag cttcggtgcg cttgtcggcg ccgaggccgc gatcaacggc gacttcttca
     3601 gcttcgagac ctacggcacg aacggcccgg cgatgagcgg cggtgccgcg tggggcggca
     3661 ccgatcacaa ctacgtcgct ccggtgcaat tcggagcgta tcgcgtcgcc ttgccgccgc
     3721 acgaagcgac aggcggtgtc gaaccgtggg cgcgcgaggt ggtgagcggc cacccgacgt
     3781 tgctcgttgc tggcgctcca cgcaacaaca acggcgacac gctgtgcact gcgcggcatc
     3841 cacggaccgc gctcgggctc tcggccgata aggagaagtt gttcttggcg gtcgtcgacg
     3901 ggcgcgcaac cggacggatc gggatgacgt gcgacgagct cggtgcgctg ctcgagggtc
     3961 tcggtgcgag cgacgcggtg aacctcgacg gcggcggttc gtcgacgatg tggctcgcag
     4021 gaaccggcgt gctcaacttc ccatcggacg gcgcacaacg tgtcgtcgca aatcacctcg
     4081 cgatcaacgc gaccggcacc ggcgcggcgc cgaactgccc gcatccgaag ttcgcggcga
     4141 cgtttgtcgg cagcgaagcg ccgatggaaa tgacgtcagg tgacgaggcc gtcgtgtttc
     4201 tcgaagcgac caatgatggc aacgcgagct gggacatcac gcagacgcgc gtcggaaccc
     4261 aagacccgca ggaccgcgac tccgcgttct tcaaagcgga gaactggatc gctccgaatc
     4321 ggccgaccgg cgccgatcac acctatgcgc ccggtgctac cggcaggttc acgtgggcga
     4381 tggtcgcgcc ggaggtcgag aagtcgacga tgttcgacga gacgttccag ctcgtgcacg
     4441 aaggggtcgc gtggttcggc gagaagcgga cgatgacgat cctcgtgcac ccgcgcagcg
     4501 ggccgaccac cgacgacggc gatggcagcg gaagcgacga tgcaccgagc aacagtggat
     4561 gcaatgcggc cgatggttca ccgacgtggc cgctggtcat gatcgtgttg ctcgcatact
     4621 ggcgccgacc tcgccctgat cgtctcgacc gagcgcgacg gtaatcccga gctctacgag
     4681 acacagcgtt agctggacgg tacgatgcta gggtggggca ggtcgcgtcg ctcggtagtc
     4741 gaggcctagc ggccacgctg tggttcgtcg tctgggggtg tgggcgcgtc aacttcgatt
     4801 cgcgtagtga cgcgaacgac gcgagcgatg cgaccggtca gctcggcccg ccgatggtcg
     4861 ttggcgagct gacggttggc ggtgttgatg ccgacgatcc gacgctgact gccgacatgc
     4921 tcgagatcta tttcgcgtcg tcgcgaccgg gcggcgtggg atcgggcgac atctggcgct
     4981 cgattcgcaa cagcgtggac gaaccatggc aaccaccggt ggtggtcacc gagctcgata
     5041 gcgcgagcgt cgaggaaagc ccggggatca cggctgacgg tctggagatg tacttcacga
     5101 gcgatcgcgg cggcggcaac gatatctggg cgagcacgcg accgacgcgc ggagacatgt
     5161 gggctgcgcc gaccgtcgtc gccgagctgt cgaccggctc gggcgacttc cagccgcagc
     5221 catcggcgac aaagctgcgc atcgtgttct atcgctcgtc gggaaaccgc gacatctacg
     5281 aggcgacgcg caccgcagtc ggcgcaacgt ggcagtcgcc cgccgtgctc gcgaacgtca
     5341 acagcgcggc cggcgatcgg agtccgtgcc tcgatggctc ggagctcacg atggtcttca
     5401 gctcggatcg cgattcggcg accgcggacg tgcaagacct ctatctcgcg acacgtgcca
     5461 gtctcaccga cccgttcggt gctgcgcagc cactgaccga cctcaactcc cagttcgacg
     5521 acgacagtgc gtggctctca tccgacggcc atgtgttgtt tttcagcagc gatcgcagcg
     5581 gtaccagcga gatttacgtg acccagcgct gactcgtggg tgtgctccgt cagctgtatt
     5641 gagcgccgcg attcggtaca ttcaggggcg tgactacccc ccgcgtgcag atccgtcggc
     5701 ttcgccccga tgcgatcgtg ccgcgctatc agaccgcaga cgcggcgggc atggatctgc
     5761 acgccgcact cgatcagccg atgcaagtcg cgcccggcgc cgtcgccgcg gtgccgaccg
     5821 ggctcgcgat ggcgatccca cgcggcttcg aaggacaggt gcggccgcgg tcgggcctcg
     5881 cgcgcaagca cgccatcacc gtcgcgaatg cgcctggaac gatcgacgcc gactaccgtc
     5941 tcgaagtcat ggtgttgctg atcaatctcg gtcgcgagcc ctatgtgatc caacccaacg
     6001 atcgcgtagc acagctcgtg atcgcgccgg tcgtgcaggc cgagcttgtc gaagtagccg
     6061 agcttgacga gacagcgcgc accggcggat tcggatcgac ggggcggtga gcgagcgatg
     6121 agcgcgcgtt cgaagaaacc aacctcgaag gggcaggcgc caccgccgtt caccgacgag
     6181 aacaagaaga ccgtcgtcta cgattcgaac aagggcaaga agtcgggcgg gcagtacgcg
     6241 gagccggatc cgaccgtcgt cgacggtccg ctcggtggcg cgaagatgta cgagaagccg
     6301 gcgccaaacg ctggaccgac cgtgctcgag aagccgcgac cgcccgttgt cgcggtgtcg
     6361 aagaaagagc agtccgcgcc gatcaaggcg atctcgatga agacgccgaa tgatcaacaa
     6421 gcgcccgcgc ccgagaagac accgatgctc gcgcggccga agctgcgcgc ggtctcggag
     6481 gtgatgccgg cgcagacgcc gcaaaacctc ggcaatctcg cgcagccgta cgatccgaag
     6541 gaagcgcgct cgcgtgcgat gcgcgagtac gtgatctggg gctgcgtcgc cgtgatcctc
     6601 gcgtcggcga tcgcgctcgt ggtctggttt gcagcgcgct agggcaggga cgtgcaggtc
     6661 gccgtgccgc cggagcgcac gcgtgcaaat ttcagcgtcg tcgagccggc gctgtcgaac
     6721 caggcccaca cgatgtcccc gttgagatga tcgcggaacg gatcgtcgcg ctcgccccac
     6781 ttcgcagtcg tgatcgtcgt cggtccttcg aggaagtttc cggtcgcgga cacgcgcgcg
     6841 agcgagtacg tggttccgct cgaccagcca acgacgtatt gctcggtcgc gtcgccgtcg
     6901 ggcttccacc gcgcgatcga cgagttcgcc tcgttgccgg tggtcgttgt cagccacacg
     6961 acggcgccgg gcgtcttgtt gttcgcgatc gacacgaagc cgatgtcttg attcatcgtc
     7021 gacgacgagt acgagctctg accgttcgtc gcggcgttct ggtgcgtgtt gaacacgagc
     7081 ttccagcctg cggtgccggg cgcgatgctg ccgagctcgc cggcgaccga gccgttgcag
     7141 ccaccatcga cgttcatgac cttctggtct tgatggttga gatagatgcc gccgatcgaa
     7201 tcggtcggga agttcgagcc ggtcgtgcct ggataacaat cggtcacgca caccggcagc
     7261 gtgaggttgg cagccggcga gaaccggagc agctcgctca tcgagtgcga acagccccaa
     7321 tcccagccga gcgtgtcggc gccggtggcc gcggcaagcc agtggatcgc atcgccctcg
     7381 tgaccgttcg cgaaccccga gatgccgtgg acatggaagt atgcgccgta cttgccgccg
     7441 ccgtactcga gccggctctc gccgatcgcg aagtcggtcg gcgcagcgag tgcgttgtcg
     7501 agcgacgtcg tccagcccgc ggtgccgttg gacgtgaagc gcgtgacgtg gagcgacttc
     7561 ggggtcggcg cttgatccca caggagcgcc gcgaagtggc cgtcgggctc ggcggcgaga
     7621 ccgcgcagcg tgaacgcgcc gaggtcgtag gtcgtgacga gttggtccat cgcgttgagg
     7681 cgggcgacgc gtgcgcgcgg gttcgtcgtc gtgccggtcg tgtagccaac cagcgtgccg
     7741 cagtcggcga acggcaccgt gtagaccggg ccgatgcgca ggctgcccat gccccagatg
     7801 cgccagttgc tgatgccggg gatgacgccc gcgggagtcg ttatcgaagc gacgacgagc
     7861 cggtcctgta gcggcgagct cgccggcacg cagtcgttgt cgttgttgct gtcgcaaccg
     7921 ggcgcgcaac acgaatcgcc gttcgtacag gcggtaatcg gcgtggtcgc gcacgccgcg
     7981 ttgcagttcg cggagctgcc gatcagcgtt ccggccatgc agatcggggc cgggcaagcg
     8041 accggacagg attcaggggg atcgcacgtt tcgttgccct cgaccgtccc gttcccgcaa
     8101 gtggtcgagc agtcgttgtc gtcggcgggc gagcagccgg caggacagca gccatcgcct
     8161 ccctcgcacg cagttactgc gaggcaagca tcatcggtgc cgccatcggt ttgtttcgca
     8221 gtttccttgc cgcagccgag cgcaagaatt ccgaggagaa cccacctcat atacggatga
     8281 tgccccattt cgcgatcagc cacgcatggt aaaaggggcc cgccgtgatt cctcgatata
     8341 gtcgacccga gctcgcacaa ctctgggacg acaaatatcg tttcgagctc tggctcgaga
     8401 tcgagctgtc ggtgtgtgag gcgatggagc agcgtggcac cgtgcccgcg ggcaccgccg
     8461 cgaaagttcg cgccgccgcc gctggcaagc tcgatcccgc gaagatcttg gagatcgaag
     8521 cgaccacacg ccacgacgtc atcgcgtttc tgacgcacgt cgagcagctc gcgggtgagc
     8581 ccgcgcgctg gctgcacctc ggcatgacct cgagcgacgt gctcgacacc tgcctcgcgc
     8641 tgcagaccgg ccgcgcactc gagaagatcg tgcagggcgt gatggggctc gcgaacgtgc
     8701 tccgcgatcg tgcacgcgag cacgcgaaga cgccgacgat cggccgctcg cacggcattc
     8761 atgccgagcc gatcacggcc ggactcgtgt tcgcacgctg gcacgccgaa gtcgtgcgcg
     8821 cgctcaaccg catcattcgc gcgcggcacg cgatcgcggt cggcaagatc gcgggtgcgg
     8881 tcggcgtcta cggcaacctc gatcccgaga tcgagcgcca ggcactcggc agcctcggcc
     8941 tcgagcccga gacggtggca acgcagatcg tcgcgcgtga tcgtcacgcg gaagtcttct
     9001 gcgcgctcgc gctgctcggc accgcgatcg agcagatcgc actcggcgtg cgccactggc
     9061 aacgcaccga ggtcggcgaa gcggaggaag gcttcggcgc cgggcaaaag ggcagctcgg
     9121 cgatgccgca caagaagaac ccgatcctca gcgagaacct cacgggcctc gcgcgcctgt
     9181 tgcgcagtta cgcgcaggcc gggctcgagg acgtcgcgct gtggcacgag cgcgatatct
     9241 cgcactcgag tgtcgagcgc gtcgcgatgc ccgacgcgac gatcctcgcc gacttcatgg
     9301 tcgcgcgcgc gaccgggctc gtgaagggac tgatcgtcca cgagcgacgc atgaaggcga
     9361 acctcgagaa gaccggcggt ctgtacttct cggaggccgt gttgctcgcg ctcgtgaaga
     9421 ccgggctgcc ccgccaggaa gcgtacgtgc tggtgcagaa ggcggcactc gccgcgatcg
     9481 ccgaggctgc gcagatgggc cacgttggcg cgctgccggg acgcttccgc gagctgctcg
     9541 gccaggattc cgaagtgacg aaacggctcg acgcgaaggc gctcgacgcg tgcttcgatc
     9601 tcgaacacca cctgcggcac gctgccgaga ttctcgatcg cgcgctcggc gcggagggtt
     9661 cccatggcaa ctgacctcga cacttcgctg cgcggccaat tgaaccagac gctgacgcac
     9721 acgccgtgga agacgatcgg cgggcgcacg ctgccgcgtt acgacggcaa ggtgcgcgat
     9781 tgctacatcg acgtcgagcg cggtgagcgt atcatcgtcg tcaccgatcg gctcagcgcg
     9841 ttcgacgcgg tggtcggcac gattccgttc aaggggcagg tgctgaacca gctcgcgcag
     9901 ttctggttcg agcagacgaa agccatcgcg ccgaatcaca tgctgcgcgt gcccgacgca
     9961 aacgtgatgg tggcgcgcga gtgcgagccg ctgcctgtcg agctcgtgat gcgctcgtac
    10021 ctgacgggcg tgacgtcgac gtcgatctgg aaggcctacg aagccggcgc gcgcacgttc
    10081 tgtggtcatc cgctgccgga aggcatgaag aagaaccagc ggctgccgaa ggcgatcctg
    10141 acgccgtcga cgaaagccgc gaaaggcgat cacgacatct cggtcagcaa ggacgagctg
    10201 ctcgcgatgg gccgcatcaa gcccgagatc ttcgagcgcg cagcggcgat cgccgagaag
    10261 ctgttcgcag cggggcaggc gcacgccgcg aagcaaggcc tcatcctcgc cgacaccaag
    10321 tacgagatgg gcctgtcgcg cgacggcacg atcgtcgtca tcgacgagat ccacacgccg
    10381 gattcgtcgc gctactggta cgccgaagac tacgagcagc gcctcgcgaa cgcggaggag
    10441 ccgcgcagcc tcgacaagga atacgtgcgg cgctggctcg cgaacgaggc gaagtggacc
    10501 ggcgatggcc cgccgcccga catgccgggc gaggttcgcg tcgaagccgc gcgtcgctac
    10561 atcgcgagct acgagatcgt gaccggccgg acgttcgtgc cggacacgcg cgaaccgatc
    10621 gcacgcattg ccgccgcact gggggctgcg tgatcaaggc gattcgacgt tttctggccg
    10681 aagcccgaag cccgaagccc gaggcccgat cgtgctgatc gttcctggcc gcgcagcact
    10741 cagcgcggca cgtaaagcga gcgtgctcgc gaagattcgt gaggtatcgc cgcacgtcac
    10801 atcgctcgag gcgcgctggg tccacatggt ggtcgcggag cgcgagctga cgaccgacga
    10861 gctcacccag ctcggtcaca tgctgagcta tggaccggcc gagccaccga cgcggccgag
    10921 ccgccggctc gagaccgtcg agaccgtcag cttcgtgatc acgccgcgta tcggcacggt
    10981 gtcgccgtgg tcgtcgaaag cgaccgatat cgcgcacgtc tgcgggctca ccgcgatcac
    11041 gcggatcgag cgcggcaccg agtggacgct ggtcggcgat gcgatcgatc tcgccgccgt
    11101 cgcgcgcgtg ctgtcggatc gcatgaccga aagcgtgatc acacgcgagc aggatctcgc
    11161 gaaggtggtc gcgcccgcgg gaacgccgcg cccgctccag ttcatccagc tcggcgcacg
    11221 cggtagcgag acgctgcgcg acgccagcac gcgtcttggc cttgcgctcg ccgacgacga
    11281 aatcgaatac ctcgtcgccc gttacaagga gctcggtcgc gacccgagcg acgtcgagct
    11341 gatgatgttc gcgcaggcga acagcgagca ttgccgtcac aagatcttca acgccgagtg
    11401 gtacctgtcc ggcgagaagc aagaccgctc gctgttccag tggatcaagc gctcgacgga
    11461 atccgcgccg gccggcgtgc tttcggcata caaggacaac gcggctgtga tcgaaggcgc
    11521 gaccgcggag cgcttcttcc cggaccccga cggcgtgtat cgcggccacc tcgagcccgc
    11581 gcacatcctc ggcaaggtcg agacccacaa tcacccgacc gcgatctcgc cgttcccggg
    11641 tgcggcgacg ggctcgggcg gcgagatccg cgatgagggt gcgacgggcc gcggcgcgaa
    11701 gccgaaggca ggtcttgtcg gcttcacggt ctccgacttg cgtatccccg acgcgctcga
    11761 gccgtgggag cgcgatgaat ggatcggctc gccggcgcgc atcgcgaccg cgctc
//
LOCUS       DASUFX010000178         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1000964, whole genome shotgun sequence.
ACCESSION   DASUFX010000178 DASUFX010000000
VERSION     DASUFX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000964"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFV99_07065"
     CDS             <1..1011
                     /locus_tag="VFV99_07065"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5059102.1"
                     /translation="IEAAGGWSTATTTSDYLAVTEQVPAHALELVLWLEAERMAGLAD
                     GITEHRLAGARDAVYGEWRAAYVDEPYALVTRELQQTLWNGKFAAQGLPVLGEGYAAH
                     VATFEAVRRFARERLVPNNAVIVVAGRFDATKARGFVEHYFGWIPTRETVTGRGDLFV
                     EPRPSAAEVTVKDAIAKVVVAARCEPGDAEIDVIAQVVAARSQRLVTAGLASEVSVDV
                     VRRRAAEFRLQATPRAGVDPAGLATALRDVVATLHAQPPDVEELSRAIASADRDLLLA
                     YENVAVRADLIATWSTIAVGTLVDKRARLQRVTPESVERTWQRWLRPPALVTVIGRPE
                     GP"
     gene            1011..2642
                     /locus_tag="VFV99_07070"
     CDS             1011..2642
                     /locus_tag="VFV99_07070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5059103.1"
                     /translation="MRVGAMRFVVVCAALACVQCGPKVRMVARDAPVPWEASGIDWTK
                     PPALGVPVPFAPPGTQTIVLPNGMRVIVVENHRLPLVAMAMVNEGAGSREDGKKPGLA
                     ALTADLLDDGAGKRTAAELRDALARGGARLETHIATDYASLEMVAPSQQLEANLALVA
                     DMVVAPRLESPDLLRVRAERAAEIEARTQRPRTIAAQVFDRVVFSAHPYAEPSEGSAK
                     LVAALTTADVRGFWARAYRPESATLIIAGDVRGAIVRQVVEQTFGTWSARTGTATGTA
                     AGTGAATGTGAATGTGTATGTGAATGTAAATGTATATGTAAATGTAAATATGTVSGGP
                     PLGRYAAQVALVDLPGARQSVVLIGGRASAAGDDKQLVDDVASSIVGGGFGARLDKEL
                     HEKLGLTLGASASYWRGRWAGSWALATTFATDKTLAGIRAALAVLDRARRDIGDDELA
                     TAKTNLIAAAQLSFETTAGTARAFERLVAQGLPVDSFATLEKRLAALTLADVRAASSW
                     SDLSIVIVGDRRKLEPVLSDLALPITHYDRDGTQP"
     gene            2702..3994
                     /locus_tag="VFV99_07075"
     CDS             2702..3994
                     /locus_tag="VFV99_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5059104.1"
                     /translation="MLWLAAPLVAQQAGSHLMGVVDAAMLGRYSDSALAAAGVGNNVY
                     FAVSCVGMGIVMGMDTVVPQALGAGRLDDARRAVGAGVRLAIIVGLLATLLLLASPIV
                     LVWADIDKDVLHEARPYLYMRALGVVPFLFTVALRSYLAAHGMTRPMVIAVIAGNIVN
                     AGLDLALIYGVDAIGLPAFGVIGAAAATTIVSILTASLYALAVRNLDRGVTRPRSTGS
                     DLKAIAHHGWPVGGQLLAEVGIFGVATVLAAHLGKVPAGAHSIALNLSSLTFSFGLGI
                     ASATSVRVGHAVGAGDLALARRRGLLGLVLGLVVMSCFAATFLLIPRLLATGFTDDST
                     VVAATIPLLQIAALFQLSDGTQAIGAGALRGRGDNKSTLIGNLLGHYVVGLPIIIGLG
                     FGAGLGAPGLWWGLSAGLTVTALFLLARFLRSTSQSRR"
     gene            complement(3966..>4835)
                     /locus_tag="VFV99_07080"
     CDS             complement(3966..>4835)
                     /locus_tag="VFV99_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059105.1"
                     /translation="GKDWKNQVEMGYAVSSQTPSLLSNFPNDPAMQAAFKSAVFPHTE
                     VVSNPFVEYTIYRANFRTIRNVDTFDLAEDLRTGPNLTIGLAQSLELLGSDYTFTRPS
                     FTAGWTFPWTRDGFVRVSAGGALRIQPGKRTEGQTTTTIDNTATAQIRAATPTTLYGR
                     LIAQVHAETRWNDTTNAFYTLGSESGLRAYDIGQFIGQRRAVGQLEWRTIPYPFWVLR
                     LGAVAFYEVGGVANSFSTMQLHQDVGVGLRMLIPQTARDLFRFDFAKPLDGSHACPIP
                     GNICFIAGFDSYF"
BASE COUNT          781 a         1582 c         1705 g          767 t
ORIGIN      
        1 atcgaagcgg caggcggctg gtcgacggcg acgacgacaa gcgattacct cgcggtcacc
       61 gagcaagtac ccgcgcacgc gctcgagctc gtgctctggc tcgaggccga gcgcatggcc
      121 ggactcgccg acggcatcac cgagcaccgg ctcgcgggcg cgcgcgacgc ggtgtacggc
      181 gaatggcgcg cggcatatgt cgacgagccg tacgcgctcg tgacgcgcga gctgcagcag
      241 acattgtgga acggcaagtt cgcggcgcag ggccttccgg tccttggcga gggctatgca
      301 gcgcacgtcg cgacgttcga agccgtgcgg aggttcgcgc gcgagcggct cgtgccgaac
      361 aatgcggtga tcgtggtcgc cggtcggttc gatgcgacga aggcgcgtgg gttcgtcgag
      421 cactacttcg gctggatccc gacgcgcgaa acggtcaccg ggcgcggcga tctgttcgtc
      481 gagccgcgac cgagcgcggc cgaggtgacg gtgaaggatg cgatcgcgaa ggtcgtggtc
      541 gcagcacgct gcgagcccgg cgacgcggag atcgatgtca tcgcgcaagt cgtcgccgcg
      601 cggtcacaga gactcgtcac tgcgggcctc gcgagcgagg tgagtgtcga tgtcgtgcgc
      661 cggcgcgcgg cagaatttcg gctgcaagca acaccgagag ctggcgtcga tccggcggga
      721 ctcgctacgg cgctccgcga cgtcgtcgcg acactgcatg cgcagccacc ggatgtcgaa
      781 gagctgtcgc gtgcgattgc gagcgccgac cgcgacctgc tgctcgccta cgagaacgtg
      841 gccgtgcgcg ccgatctgat cgcgacgtgg agcacgatcg cggtcggcac gcttgtcgac
      901 aagcgcgctc gcctgcaacg ggtgacgccc gagagcgtcg agcgcacgtg gcaacgctgg
      961 ctgcgtccgc cggcactcgt cacggtgatc ggccggccgg agggaccgtg atgcgcgtcg
     1021 gagcgatgcg gttcgtcgtc gtgtgcgctg ccctcgcctg cgtgcagtgc gggccgaagg
     1081 tgcgcatggt cgcgcgcgat gcgccggtgc cgtgggaggc atcgggcatc gactggacga
     1141 agccgccggc gctcggtgtg ccagtaccgt tcgcgccccc agggacgcag acgatcgtgc
     1201 tcccgaacgg catgcgcgtc atcgtcgtcg agaatcatcg gctgccgctc gtcgcgatgg
     1261 cgatggtgaa tgagggcgcc ggcagtcgcg aggacggcaa gaagccgggg ctcgcggcgc
     1321 tcacggccga cctcctcgac gacggtgccg ggaagcggac ggcagcggaa ctgcgcgatg
     1381 cgctcgcgcg cggcggcgca cgcctggaga cgcatatcgc aaccgactac gcgagcctcg
     1441 agatggtcgc accgagccag cagctcgagg cgaatctcgc gctcgtcgcg gacatggtcg
     1501 tcgcaccgcg actcgagtct ccagatctct tgcgcgtacg cgcagaacga gccgcggaga
     1561 tcgaggcacg gacgcagcga ccgcggacga tcgcggccca ggtgttcgat cgggtcgtgt
     1621 tcagcgcgca tccgtatgca gagccgtcgg aaggaagcgc gaagctcgtc gcagcgctga
     1681 ccaccgccga cgtgcggggg ttttgggcac gggcatatcg tccggagagc gcgaccctga
     1741 ttattgcggg ggatgttcgc ggtgcgatcg tgcgccaggt cgtcgagcag acgttcggca
     1801 catggagcgc gcgaaccgga accgcaacag gaaccgcagc aggaacgggc gccgcaacag
     1861 gaacgggagc cgcaacagga acgggaaccg caacaggaac gggagccgca acaggaacgg
     1921 cagccgcaac aggaacggca accgcaacag gaacggcagc cgcaacagga acggcagccg
     1981 caacagcaac aggaacggtt tcggggggcc ctcctctcgg acgatatgca gcgcaggtcg
     2041 cgctcgtcga tctgccggga gcacggcaga gcgtcgtgct gatcggcggc cgagcgtcgg
     2101 cggcgggcga cgacaagcag ctcgtcgatg atgtcgcgag ctcgatcgtc ggcggcgggt
     2161 tcggcgcgcg cctcgacaag gagctgcacg agaagctcgg cctgacactc ggcgcgagcg
     2221 cgagctactg gcgcggccgc tgggcgggct cgtgggcgct cgcgacgacg ttcgcgaccg
     2281 acaagacgct cgccggcatt cgcgcagcgc tcgccgtact cgatcgcgcg cgcagggata
     2341 tcggcgacga cgagctcgcg accgcgaaga cgaacctgat cgcagctgcg caactgtcgt
     2401 tcgagaccac cgcgggcacc gcgcgcgcgt tcgagcgcct cgtcgcgcaa ggcttgccgg
     2461 tcgatagctt tgcgacactc gagaagcggc tcgccgcgct cacgctcgcg gacgtacgcg
     2521 cggccagcag ctggagcgat ctctcgatcg tcatcgtcgg tgatcgccga aagctcgagc
     2581 ccgtgctatc cgacctggcc ctgccgatca cgcactacga tcgcgacgga acgcagccat
     2641 gaacgaccac gcgctcgcgc aagctagtac cgacacgcgc gggcgctccg aggcttccgc
     2701 gatgctctgg cttgccgcgc cactcgtcgc gcagcaggcc ggctcgcacc tgatgggcgt
     2761 cgtcgacgcg gcgatgctcg gccgctacag cgactcggca ctcgcggcag ctggcgttgg
     2821 caacaacgtc tacttcgccg tcagctgcgt cggcatgggc atcgtgatgg ggatggacac
     2881 ggtcgtgccg caggcacttg gcgcgggtag gctcgacgat gcacggcgcg ccgtcggcgc
     2941 cggtgtgcgt ctcgcgatca ttgtcgggtt gctcgcgacg ctgctcttgc tcgcgtcacc
     3001 gatcgtgctc gtgtgggcgg acatcgataa agacgtgctg cacgaagcgc ggccgtactt
     3061 gtacatgcgc gcgctcggcg tggtgccgtt cttgtttacg gtcgcgctgc gctcgtatct
     3121 cgccgcgcat ggcatgacgc gtccgatggt gatcgcggtg atcgcgggca acatcgtcaa
     3181 tgctgggctc gacctcgcgc tgatctacgg cgtcgacgcc atcggtctac ctgcgttcgg
     3241 cgtgatcggt gccgccgcgg caacaacgat cgtctcgatc ctgacggcga gcctgtacgc
     3301 gctcgcggtg cgcaacctcg atcgtggggt cacgcgacca cgatcgaccg gctccgattt
     3361 gaaggcgatc gcgcaccacg ggtggcccgt cggcggtcag ttgctcgcag aggtcggcat
     3421 ctttggcgtc gcgaccgtgc tcgccgcgca cctcggcaag gttccggccg gcgcacacag
     3481 catcgcactc aatctgtcga gcctgacgtt ctcgttcggg ctcggcattg cgagcgcgac
     3541 gtcggtgcgc gttggtcacg cggtcggcgc tggcgatctc gcgcttgcac gccggcgcgg
     3601 cttgctcggt ctcgtcctcg gtctcgtggt gatgtcctgc ttcgcggcga cgttcctgtt
     3661 gatcccgcgg ctgctcgcga ccggcttcac cgacgattcc accgtcgtcg ccgccacgat
     3721 tccgctgctg cagatcgccg cgctgttcca gctctccgac ggtacgcaag cgatcggcgc
     3781 cggtgcgttg cgcggccgcg gcgacaacaa atcaacgctg atcggcaacc tactcggcca
     3841 ctacgtggtc ggactcccga tcatcatcgg gctcggcttc ggcgctgggc tcggcgcacc
     3901 cgggttgtgg tggggcttgt cggcaggcct caccgtgacc gcactgttcc tcctggcgcg
     3961 cttcctcaga agtacgagtc aaagccggcg atgaaacaga tgttgcccgg aatcgggcac
     4021 gcgtggctgc catcgagcgg cttcgcgaag tcgaagcgga acaagtcgcg tgcggtctgc
     4081 gggatcaaca tgcgaaggcc gacgccgaca tcctggtgca gctgcatcgt gctgaacgaa
     4141 ttcgcgacgc cgccgacttc gtagaacgca acggcaccga gccgcagcac ccagaacgga
     4201 tacggaatcg tgcgccactc gagctgaccg acggcgcggc gctggccaat gaactgaccg
     4261 atgtcgtacg cgcgcaagcc ggactccgag cccagcgtgt agaacgcgtt cgtcgtgtcg
     4321 ttccagcgcg tctccgcgtg cacctgcgcg atcaagcggc cgtagagcgt cgtcggtgtc
     4381 gcagcgcgaa tctgcgcggt cgccgtgttg tcgatcgtcg tcgtggtttg gccctcggtg
     4441 cgcttgccag gctggatgcg cagcgcaccg cctgcggaca cgcgcacgaa gccgtcgcgc
     4501 gtccatggaa acgtccagcc cgccgtgaac gacggccgcg tgaacgtgta gtcagagccg
     4561 agcagctcga ggctctgcgc caacccgatc gtcaggttcg gtccggtgcg caggtcctcg
     4621 gcgaggtcga acgtgtcgac gttgcggatg gtgcgaaagt tcgcgcgata gatcgtgtat
     4681 tcgacgaacg ggttcgacac gacctcggtg tgcggaaaca ccgcgctctt gaaggccgct
     4741 tgcatcgccg ggtcattcgg aaagttcgac aacagcgagg gcgtctggct cgacacggcg
     4801 tagcccatct cgacctggtt cttccagtcc ttgcc
//
LOCUS       DASUFX010000179         9354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_27808,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000179 DASUFX010000000
VERSION     DASUFX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27808"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..558)
                     /gene="tsaE"
                     /locus_tag="VFV99_07085"
     CDS             complement(106..558)
                     /gene="tsaE"
                     /locus_tag="VFV99_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5059106.1"
                     /translation="MLTLADAAATTRAGERLAAALKGGDAIALVGDLGAGKTTLVAGL
                     VAALGGGDAQSPTFSLVNEYAGGRLIVWHVDLYRIDIPAELPELGLDDVIGDPRGIVV
                     VEWADKFDVMPGDHLRLELAHAGNARALTATGTGPRGRALADAVLASA"
     gene            complement(568..1206)
                     /locus_tag="VFV99_07090"
     CDS             complement(568..1206)
                     /locus_tag="VFV99_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059107.1"
                     /translation="MRNLICAHMPYVLLLSLVTAACSSDEVDSDEEARRAYLALDGSI
                     DKSLNLGFTGYNLATSANIDAQMTTGTKAGTLTVDGQVDKGASDNKGMRLYIAMVGYD
                     DGDVVYNDDGDTVHVVFDTSVDMTAQPYLDLHLMNVPTGTMDGTLGANTAMTGVYQLS
                     GDIEGSLTIEVTITANLMAGTGTDVLRVPGSTHILGTATNSDGGVYDIDVTR"
     gene            complement(1304..1915)
                     /locus_tag="VFV99_07095"
     CDS             complement(1304..1915)
                     /locus_tag="VFV99_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059108.1"
                     /translation="MPNRVCGLMPYVLPVALACAACSGVDSDEQAHRAFLALDQSIVR
                     TLNLAFQGFNMATNTTIAPQTAEGNHGGTLTVDGTIEQSAAMKTLRLDVAMVGYDDGD
                     VLFNDSDTVHVKFDTATDATMLPHLDLALTTNAFSGTLDANASMTGLYQLRGDLTGTL
                     ALDVMISGYTMVGNGTERVPGSTQIVGTATNGDGGVFEINITR"
     gene            2081..3796
                     /locus_tag="VFV99_07100"
     CDS             2081..3796
                     /locus_tag="VFV99_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059109.1"
                     /translation="MEIDRSWQRTVFVAGLDGNLVARSELVNTLVGERVLDPYRRALG
                     AAPLRLKRGSVMRYRVVRMDDTTDEKLAPDPESRDGDAEREQRASEARDELGTHETAL
                     ATIENKLPLIVRERPPIWGFWLWPLRWLLGFLHRRTTSSWRVTKQLVADAKRKLASID
                     EFKLKREERERAARETYYSELRLLCGGGPEGNGVREVELTIDRALPERVELVELMGEL
                     RASADIDAVLVVERDGLYAPNPGGDKVMLGPLSETIPDLPAVLERARALTLARRAIAK
                     LATARTDIEQQVDQVERKFQERIQKLAKLALPIDLARFRAAQLDRMRPMLRASVNAVM
                     EHAAVHMGTELANLGTEWMAAIQNATTSDELKAAVAKIEGWPTSAMRIAEEVRVLVMG
                     GAGGIARDLYAETVSPLRAHGLPEDHLKTPRRAPTVAPVTILDSLANPTSFTLGGNWF
                     AGLFKSFDAKKADIREKVHARIEKIRDVAAAELLNAEPELHGAVIITLGAQLDTAIEA
                     QQAWHQKALEEEHAAIGKEREKLMPLMRSGDALTKASNQLVHAVNALQAERPAVAAAA
                     VAAAS"
     gene            complement(3793..4728)
                     /locus_tag="VFV99_07105"
     CDS             complement(3793..4728)
                     /locus_tag="VFV99_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059110.1"
                     /translation="MAMRLTLGEMLGAGTRGVGRYTGTLLAVFLVQTFITIVCMIAIW
                     FALSRAYSHLPMWDEAVDGDLVALIFCIRYGKASLLASAGIAFGALLLWQVVSWFLVG
                     GIYGVLAQRPEGRAETAKCFGASGASTFLAYARLMLCSIPGWVLVVIALGLGLSQFAS
                     ESRLQHALTVPQLLGPIVLGSLPALVLLHFFWTVTDYARVELSLRQETHEPSVVMTYA
                     RTIMFVLRRPLTLAHGALGWLAFALISIGYAWLAHGHPMYGAEGAVTLFVIRQGVALA
                     RSAIRFGVLAGQLELGKTRALPPRRVEVKTETKKS"
     gene            complement(4728..6686)
                     /locus_tag="VFV99_07110"
     CDS             complement(4728..6686)
                     /locus_tag="VFV99_07110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5059111.1"
                     /translation="MKKLPKADGSPALRGGGAPRSPQIASYNIDARLDAVRHQVTGKE
                     TLVWMNTGQSAVDVLPFHLYLNAFKNEQSIFMKASKGEMRGAKASDTGWGWIEITSLK
                     IGGAEQVAKLRYPNLPDETVVELPLAEPVQPGSSIEIAIEFTDQLPEVFARTGYKGDF
                     HLVGQWFPKIGVRVSAPSGDRWECQPLHINTEFFADFGKYDVALTVPSTHVVAATGVL
                     VKAADLGGQMRQYTYHAEDVHDFVWMADPYMEIDKNTGRTPYIGQAKVDDGIVEVRVY
                     ARPEQAQFARRHLQAAIGAIEKFSNYFVPYPWPIMTVIDPPVDAADGAGGMEYPTLVT
                     TAGDSVFTRPGVRIPEYVTVHEVGHNWFQGILASNEPVEAWLDEGMNEWADLHVMQDL
                     YGPRANGIEWGGWQADIGWMREAIAGGDFDNLPSPIATAAYAFVDNKAYGQGTYAGTM
                     AVMTTLEKLVGSTKFLAAMKAYAKEWAFKHPTGRDLFAVLERELGMDLGWYFGPTFEQ
                     VGGHKLEVRTADCDKVHEARGVFGEGPGRKTKTEDQAPDTGTYQCEVIVQNTGVVHVP
                     VEVEFKFADGSTQRETWDDRGNGNWKRWVIERSSKLVEVRLDPDRKIMFGNPVTDQLR
                     IDGDGSASLRAAARIASWTQGFMQLVGP"
     gene            6817..7740
                     /locus_tag="VFV99_07115"
     CDS             6817..7740
                     /locus_tag="VFV99_07115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059112.1"
                     /translation="MERIGNYRIDCERSRDATGVVYEARHLVLPRRAILKVMEEKDAR
                     IAAVQLLREACILEALEHPGVVRVYESGLLPDRRPWFAHERVDGATVKDLLAGGALDP
                     AHASALLRDLAEVLEHAHRRGVIHCGLRPDRIAVTGRTRGFPLCIVDWADARTHDTSA
                     PPPQHAFPDYAAPELVNGGLLDDRADVFALGVIGYQALTGELPFDGVWIATVGDGTTQ
                     HAATEVRCPDAPPELCSVIDQMLAWDRWDRPSSAEAFADLAAIAAELAPAPPRPPSTV
                     RIRRPKWTPSLPLGDGLPDIIALDVTDEKSE"
     gene            7761..8168
                     /locus_tag="VFV99_07120"
     CDS             7761..8168
                     /locus_tag="VFV99_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone"
                     /protein_id="HEX5059113.1"
                     /translation="MDDVVVDHRARIAEFFDRFGRERNLNLPPLGDDGVGMIQRGSAV
                     VTIHVLADKNILLLLSRVAKQPTIDEARAKKLLVASFTETGDAAFALHPNTGDLYLRI
                     LRSLDGLDYEEFEDIVHSIAQTADLWDDKLADL"
     gene            complement(8457..>9354)
                     /locus_tag="VFV99_07125"
     CDS             complement(8457..>9354)
                     /locus_tag="VFV99_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5059114.1"
                     /translation="SPHGTLATHRHPASGAGPEPLRLVIGSEGALGVITEAWMRIVPR
                     PRWRASASVAFKDFSDGVAAARSLAQSGLQPANARLLDPNEARLHRVRFDGTAVLLVG
                     FESADHPMSPWLARAIELAKDHGGKVVSGPTEKDDAAKTGEAGAWRQAFFDAPYLQSA
                     LLSIGVISDTFETACTWTAFPELHARITAAVSRALDEECGGGIVACRFTHVYSDGPAP
                     YYTFIGAAKPGGELAQWRAIKAAASEALAASGGTITHHHAVGRVHKPWYDRERPALFG
                     DALVAVKRTLDPRGIMNPGVLV"
BASE COUNT         1622 a         3136 c         3059 g         1537 t
ORIGIN      
        1 tcccgcgcgg gatcttgtag acgaccttcg gatcgaagct gacttctgca cgcgcgctcg
       61 tcgcggtcgt cacgcacaga agtgccgcaa gtaccaacca acgcatcacg cagaagctag
      121 cacagcgtcg gcgagagctc gcccgcgcgg cccggtgccg gtggcggtca gcgcccgtgc
      181 gttgccggcg tgggcgagct cgaggcggag gtggtcgcca ggcatgacat cgaacttgtc
      241 ggcccactcg acgacaacga ttccgcgagg atctccgatc acgtcgtcga gcccgagctc
      301 gggcagctcg gctggtatgt cgatgcgata gaggtcgacg tgccagacga tcagccggcc
      361 accggcgtat tcgttgacga gcgagaacgt cgggctctgg gcatcgccgc ccccgagcgc
      421 ggcgaccagg ccggcgacca gcgtggtctt gcccgcgccg aggtcaccga cgagcgcgat
      481 cgcgtcgccg cccttcaagg cagcggcaag gcgctcgccg gcgcgcgtcg tggcggctgc
      541 gtcggcgagc gttagcacgc ctggagctta gcgcgtgacg tcgatgtcgt agacgccgcc
      601 gtcgctattc gtcgccgtac ccagaatatg ggtcgacccg ggcacgcgca gcacgtcggt
      661 gccggtaccc gccatcaggt tcgcggtgat cgtgacctcg atcgtcaacg agccctcgat
      721 atcgccgctc agttgataca cgcccgtcat ggcagtgttg gcgccgagcg tgccgtccat
      781 cgtaccggtc ggcacgttca tgaggtgaag atcgaggtac ggctgggccg tcatgtcgac
      841 cgacgtatcg aacacgacgt gcaccgtgtc gccgtcgtcg ttgtagacca cgtcgccgtc
      901 gtcgtagccg accatcgcga tgtacaggcg catgcccttg ttgtccgacg cacccttgtc
      961 gacctgccca tcgaccgtga gcgtgccggc tttggtaccg gtcgtcatct gcgcgtcgat
     1021 gttcgcgctc gtcgcgaggt tgtagccggt gaagccaagg ttcaggctct tgtcgatcga
     1081 tccgtcgagt gcgaggtacg cgcgacgtgc ttcttcgtcg gaatcgacct cgtcactgct
     1141 gcacgcagcg gtcacgaggg agaggagcag gacgtagggc atgtgcgcac aaattaagtt
     1201 ccgcatacga agatcgtatc cacgtcctgt cacgagcgcg atgtgcgaga agagcgctcc
     1261 aagcacttac gactgtgcaa tttccggacg gcgttacgca tgctcagcgc gtgatgttga
     1321 tctcgaagac gccaccgtca ccgttcgtgg cagtaccgac gatctgcgtc gagccaggca
     1381 ctcgctcggt accgttaccg accatcgtgt agccgctgat catgacgtcg agcgcgagcg
     1441 tgcccgtcag atcgccgcgt agctgatata gcccggtcat gctcgcgttc gcgtcgagcg
     1501 tgccgctgaa cgcgttcgtg gtcagcgcga gatcgagatg cggcagcatc gtcgcatcgg
     1561 ttgcggtatc gaacttgacg tgaacggtat cgctgtcgtt gaagagtacg tcgccgtcgt
     1621 catagccgac catcgccacg tcgaggcgca acgtcttcat cgcagccgac tgttcgatcg
     1681 tgccgtcgac cgtaagcgtg ccgccatgat taccctcggc tgtttgaggc gcgatcgtcg
     1741 tattcgtcgc catattgaag ccctgaaacg cgaggttcag ggtccgcacg atcgattgat
     1801 cgagcgcgag aaatgcacga tgtgcttgct cgtcggaatc gacgccgctg cacgccgcgc
     1861 acgcgagtgc gacgggcagg acgtagggca tcagcccaca aacgcgattc ggcatcccac
     1921 catcctatct cgcaccgtgc catgagcgcg atatgcgaga gctgcgatac cctgacacgc
     1981 aggtgtcgtg actgcgcgta cggttgtcca acaggcgctg accgcactgc ggagcgtgtc
     2041 gtttccagac gacgtgactc gcacgatgcg cgatgccgcc gtggagatcg accggtcctg
     2101 gcaacgcacg gtctttgtcg ccggcctcga tggcaacctc gtcgcgcgca gcgagctcgt
     2161 gaacacgctg gtcggcgagc gcgtgctcga tccgtaccgc cgtgcgctgg gcgccgcgcc
     2221 cctgcggctc aaacgcgggt ctgtcatgcg ctaccgcgtc gtacgcatgg acgacacgac
     2281 ggacgagaag ctcgcgcctg atccagagtc gcgcgatggc gacgccgagc gcgaacagcg
     2341 cgccagcgaa gcacgcgacg agctcggcac gcacgagacc gcgctcgcga cgatcgagaa
     2401 caagctgccg ctgatcgtgc gcgaacggcc acccatctgg gggttctggc tgtggccgct
     2461 gcgctggctg ctcggcttct tgcatcgccg caccactagc tcgtggcggg tgacgaagca
     2521 gctcgttgcc gacgccaaac gcaagctcgc gagcatcgac gagttcaagc tgaagcgcga
     2581 ggagcgcgag cgcgctgcgc gcgagacgta ctacagcgag ctgcgcctgt tgtgtggcgg
     2641 tggcccggaa ggcaatggcg tgcgcgaagt cgagctcacg atcgaccgcg cgttgcccga
     2701 gcgcgtcgag ctcgtcgagc tgatgggcga gctgcgtgca tccgccgaca tcgacgccgt
     2761 gctcgtggtc gagcgcgatg gcttgtacgc gccaaacccc ggcggcgaca aggtcatgct
     2821 cggtccgctc agcgaaacaa ttcccgatct gcctgcagtg ctcgaacgcg cacgcgcact
     2881 cacgctcgca cggcgcgcga tcgcaaagct cgcgacggcg cgtaccgaca tcgagcagca
     2941 ggtcgaccag gtcgagcgca aattccagga gcggatccag aagctcgcaa agctcgcgct
     3001 gccgatcgac ctcgcgcgct tccgcgcggc acagctcgac cgcatgcggc cgatgctccg
     3061 cgcgagcgtg aacgcggtga tggaacacgc cgcagtgcac atgggcaccg agctcgcgaa
     3121 cctcggcacg gaatggatgg ccgcgatcca gaacgcgaca acgagcgacg agctcaaggc
     3181 cgcggtggca aagatcgaag gctggccaac ctccgcgatg cgcatcgcgg aagaggtgcg
     3241 cgtgctcgtg atgggtggtg cgggcggcat cgcacgcgac ctctacgcag aaacggtgtc
     3301 gccgctgcgt gcgcacggac taccggagga ccacctgaag acaccgcgcc gcgcaccgac
     3361 ggtcgcgccg gtcacgatcc tcgactcgct cgcgaatccg acctcgttca cgctcggcgg
     3421 taactggttt gccggcttgt tcaaatcgtt cgacgccaag aaggcagaca tccgagagaa
     3481 ggtgcacgcg cgcatcgaaa agatccgcga cgtcgctgcg gccgagctgc tcaacgcaga
     3541 gcccgagctg cacggcgcag tcatcatcac gctcggcgcg cagctcgaca ccgcgatcga
     3601 agcgcagcag gcctggcacc agaaagcgct cgaagaggag cacgcggcga tcggcaagga
     3661 gcgcgagaag ctgatgccgc tcatgcgcag cggcgacgcg ctcaccaagg cgagcaatca
     3721 gctcgtacat gccgtcaacg cgctgcaggc cgagcgccct gccgtcgcgg cggcggcagt
     3781 cgcagcggca agctagctct tcttcgtctc ggtcttcact tcgactcgac gcggcggcag
     3841 cgcgcgcgtc ttgccgagct cgagctggcc ggcgaggacg ccaaatcgga tcgccgagcg
     3901 cgcgagcgcc acgccctggc gaatcacgaa cagcgtgacc gcgccctcgg cgccgtacat
     3961 cggatggccg tgcgcgagcc acgcgtagcc gatcgagatc agcgcgaacg cgagccagcc
     4021 gagtgcaccg tgtgcgagcg tcagcggacg gcgcagcacg aacatgatcg tgcgcgcgta
     4081 cgtcatcacg acgctcggct catgagtttc ctgccgcagc gacagctcga cacgtgcgta
     4141 gtcggtgacg gtccagaaga agtggagcag cacgagcgca ggcagcgagc caaggacgat
     4201 cggaccgagc aattgcggca cggtcaacgc gtgctgtagc cgcgactcgg atgcgaactg
     4261 gctgagcccg aggccgagcg cgatgacgac gagcacccag ccgggaatcg agcacagcat
     4321 gagccgcgca tacgcgagga acgtgctcgc accgctcgcg ccaaagcact tcgcggtctc
     4381 cgcgcggccc tccgggcgct gcgcgagcac gccgtagatg ccaccgacga ggaaccacga
     4441 tacgacctgc cagagcagca gcgcgccgaa agcgatgccc gcgctcgcga gcaagctcgc
     4501 cttgccgtag cggatgcaga agatgagcgc gacgaggtcg ccgtcgacgg cctcgtccca
     4561 catcggcaga tgcgagtacg cgcgactgag cgcgaaccag atcgcgatca tgcagacgat
     4621 cgtgatgaac gtctgcacga ggaatactgc gagcagcgtg ccggtgtaac gaccgacgcc
     4681 gcgcgtgcct gcgccgagca tctcgccgag cgtgagtcgc atcgccatta cggccccacc
     4741 agctgcatga acccttgcgt ccacgacgcg atccgcgccg ccgcgcgcag cgacgccgag
     4801 ccatcaccgt cgatgcgcaa ctgatcggtt accgggttgc cgaacatgat cttgcgatcc
     4861 ggatcgaggc gcacctcgac gagcttgctg ctgcgttcga tcacccagcg cttccagttg
     4921 ccgttgccgc gatcgtccca ggtttcgcgc tgcgtcgaac catcggcgaa cttgaattcg
     4981 acctcgaccg gcacgtgcac gacgcccgtg ttctgcacga tcacctcgca ttggtacgtg
     5041 ccagtgtcgg gcgcctgatc ttcggtcttc gtcttgcgac ccggtccctc gccgaacaca
     5101 ccgcgcgcct cgtggacctt gtcgcagtcg gcggtgcgca cctcgagctt gtgaccgccg
     5161 acctgctcga acgtcgggcc gaagtaccag cccagatcca tgccgagctc gcgctcgagc
     5221 accgcaaaca ggtcgcgacc ggtcggatgc ttgaacgccc actccttcgc gtacgccttc
     5281 atcgcagcga ggaacttcgt gctgccgacg agcttttcga gcgtcgtcat caccgccatc
     5341 gtgccggcgt acgtgccttg gccgtacgcc ttgttgtcga cgaacgcgta cgccgcggtt
     5401 gcgatcggtg atggcaggtt gtcgaaatcg ccgccggcga tcgcctcgcg catccagccg
     5461 atgtccgcct gccaaccacc ccattcgatg ccattcgcgc gtggcccgta gaggtcctgc
     5521 atcacgtgca gatcggccca ttcgttcatg ccctcgtcga gccacgcctc gacgggctcg
     5581 ttcgacgcga ggatgccctg gaaccagttg tgaccgacct cgtgcacggt gacgtactcg
     5641 gggatgcgga caccggggcg cgtgaacacc gaatcgccgg ccgtcgtgac gagcgtcgga
     5701 tactccatgc cgccggcgcc atcggccgcg tcgaccggtg gatcgatcac ggtcatgatc
     5761 ggccacggat acggcacgaa gtagttgctg aacttctcga tcgcgccgat cgctgcctgc
     5821 agatggcgac gagcgaactg cgcctgctcg ggccgagcgt acacgcgaac ctcgacgatg
     5881 ccgtcgtcga ccttcgcctg gccgatgtac ggcgtgcggc ccgtgttctt gtcgatctcc
     5941 atgtacgggt cggccatcca cacgaagtcg tgcacgtcct ccgcgtgata cgtgtactgg
     6001 cgcatctgac cgccgaggtc cgccgccttg acgagcacgc cggtcgcggc gaccacgtgc
     6061 gtgctcggta ccgtgagcgc gacgtcgtac ttgccgaagt cggcgaaaaa ttcggtgttg
     6121 atgtggagcg gctggcactc ccagcgatcg ccgctcggcg ccgatacccg cacgccgatc
     6181 ttcgggaacc actgcccgac gaggtggaaa tcgcccttgt acccggtacg cgcaaacacc
     6241 tcggggagct ggtccgtgaa ctcgatcgcg atctcgatcg acgagccagg ctgcaccggc
     6301 tcggcgagcg gcagctctac gacggtctcg tccggcagat tcggatagcg cagctttgcg
     6361 acttgctcgg cgccgccgat cttgagcgac gtgatctcga tccagcccca gccggtatcg
     6421 ctcgctttcg cgccgcgcat ctcgcccttc gacgccttca tgaagatcga ttgctcgttc
     6481 ttgaacgcgt tgagatagag atgaaacggc aggacgtcga cggcgctctg ccctgtgttc
     6541 atccagacga gcgtctcttt gcccgtcacc tgatgacgca cggcatcgag gcgggcatcg
     6601 atgttgtagc tcgcgatctg cggagatcgc ggagcaccgc cgccgcgcag cgcagggctg
     6661 ccatcggcct tcggaagctt cttcacgcgc gcaggaccat ggtcaggccg gcaagccgcg
     6721 accagtgcca ggagaacgac gacggcccga agcacgcctt tctataccct aaaaaggggc
     6781 tactcccacg agcggcgact tgatatgcct gcggccgtgg agcggatcgg gaactaccgc
     6841 atcgactgcg agcgcagtcg ggatgcgacc ggcgtggttt acgaggcacg tcacctcgtt
     6901 ttgccccgcc gcgcgatcct caaagtcatg gaggaaaagg acgcgcgcat cgccgcggtc
     6961 cagcttctcc gcgaggcatg catcctcgaa gcgctcgagc atcccggcgt cgttcgcgtg
     7021 tacgaatctg gcctacttcc agatcgccgc ccgtggttcg cgcacgagcg cgtcgacggc
     7081 gcgacggtca aggatctgct cgcgggcggt gcactcgatc ccgcgcacgc gagcgccctg
     7141 ctccgcgatc tcgccgaggt tctcgagcac gcgcaccgcc gcggcgtgat ccactgcggg
     7201 ctccgccccg atcgcatcgc ggtcaccggc cgcacgcgcg gcttcccgct gtgcatcgtc
     7261 gactgggccg acgcgcgcac gcacgacacg agcgcgccgc cgccgcagca cgcattcccc
     7321 gactacgccg cgccggagct cgtcaacggc ggcttgctcg atgatcgcgc ggacgtgttc
     7381 gcgctcggcg tgatcggcta ccaggcgctg accggcgagc tgccgttcga cggcgtgtgg
     7441 atcgctaccg tcggcgacgg caccacgcag cacgcggcga ccgaggtgcg ctgtcccgac
     7501 gcgccgcccg agctctgcag cgtgatcgat cagatgctcg cgtgggaccg ctgggatcgg
     7561 ccgagcagcg ccgaggcgtt tgccgacctt gccgcgatcg ctgccgagct cgcacctgcc
     7621 ccgccgcgcc cgccgagcac ggtgcggatc cgccggccga agtggacacc gtcgctgccg
     7681 ctgggcgacg gattgcccga tatcatcgct ctcgacgtca ccgacgagaa gtccgagtaa
     7741 acctcgtgta gattccggcc atggatgacg tggtcgtgga tcaccgagct cgcatcgcgg
     7801 agttcttcga tcggttcggg cgcgagcgga acttgaacct gccgcccctg ggcgacgacg
     7861 gcgtcggcat gatccagcgc ggctcggcgg tggtgacaat ccacgtgctc gccgacaaga
     7921 acatcttgct attgctatcg cgcgtcgcga agcaaccgac gatcgacgag gcgcgcgcga
     7981 agaagctgct cgtcgcgtcg ttcaccgaaa ccggcgacgc ggcgttcgcg ttgcatccga
     8041 acaccggcga cttgtactta cgcatcctgc gcagcctcga cggactcgac tacgaggagt
     8101 tcgaggacat cgtccactcg attgcgcaga ccgccgatct ctgggacgac aagctcgccg
     8161 atctctagac ctggagtgcg agacgcacca tcccgagtgc attcgtccgc cgttggagtg
     8221 cgaatggcgc ctccggcgcc gcacgacgtg ttccagatct aacgccaaga agccaaggcg
     8281 ccaaggacgc caccagaagg tggacttctt cgacgcggtc tgcagacacg atcgtgcgag
     8341 cagaacgcga tcccgaaacc ttcctcttgg cgttcttggc gttcttggcc gcttggcgtt
     8401 tcgtctcgga gcggcgaagc cgcagacggt tccttcgagg ccatgccgct acactgctag
     8461 acgagcacgc cgggattcat gatcccacgc gggtcgagtg tgcgtttgac cgcgacgagc
     8521 gcgtcaccga acagcgccgg gcgctcgcgg tcgtaccagg gtttgtgcac gcggccgact
     8581 gcgtggtggt gcgtgatcgt gccgccactt gcggcaagcg cttcgctcgc ggctgctttg
     8641 atcgcgcgcc attgcgcgag ctcgccaccc ggtttcgccg cgccgatgaa cgtgtagtac
     8701 ggcgccggcc cgtcggaata gacgtgggtg aagcgacacg cgacgatgcc gccgccgcat
     8761 tcctcgtcga gcgcgcgcga gacggcagcc gtgattcgtg cgtgcagctc ggggaacgcg
     8821 gtccacgtgc acgcggtctc gaacgtgtcg ctgatgacgc cgatcgagag caacgcggac
     8881 tggaggtatg gcgcgtcgaa gaacgcctgg cgccacgcgc cggcctcgcc ggtcttcgcg
     8941 gcgtcgtcct tctcggtcgg cccggagacg accttgccgc catgatcctt cgcgagctcg
     9001 atcgcgcgag cgagccatgg cgacattgga tgatcggcgg actcgaaacc gacgagcagc
     9061 actgcggtgc cgtcgaagcg cacgcggtga aggcgcgctt cgttcggatc gagcaggcgc
     9121 gcgtttgcgg gttggaggcc ggattgcgcg agcgagcgag ccgcggcaac accgtcggag
     9181 aagtccttga acgcgacact tgccgaggcg cgccagcgcg gtcgcggcac gatgcgcatc
     9241 catgcctcgg tgatcacgcc gagcgcacct tcgctgccga tcacgagacg cagtggctcg
     9301 ggtccagcgc ccgatgcggg atggcgatgc gtcgcgagcg tgccatgcgg actg
//
LOCUS       DASUFX010000180        12048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1557543, whole genome shotgun sequence.
ACCESSION   DASUFX010000180 DASUFX010000000
VERSION     DASUFX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12048
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557543"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..773
                     /locus_tag="VFV99_07130"
     CDS             66..773
                     /locus_tag="VFV99_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059115.1"
                     /translation="MIPTLVAVVACLVLAVLSELARRGAARVLGATPKRRALAMFAGS
                     LAIYVAIVVIAFVYFRTEGVATTQIEYVVQSVPDGYPASGKLEAGDRVIAFDGEPMYL
                     SLSTLVDERNGAPVRLTFRRNGATHDVTLQPVGHDGHWVLGFRPLADHARTTDGALGR
                     ALGFPVTQLEQLVPPPASDRADPGGPKRIIDIQLASEPSFGVKASRQTLLLATLLLVL
                     SLVTDVVRAIRTLRARA"
     gene            complement(777..1436)
                     /locus_tag="VFV99_07135"
     CDS             complement(777..1436)
                     /locus_tag="VFV99_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059116.1"
                     /translation="MKKLIMVAALIAAPISAHAQDLNYGAVEDGTDVATLTAGAEQGF
                     VVGAGYARAISVADRLLVLGGDLELDWAEIDIDDFRMQAGASAPIVTHRRLQLIGSLA
                     GVVRGTNNDAARMINVGVDAAVLGGYYAPRWFVAGEVGFDWAGATHITHSDTYRMVVY
                     ADAKDGWYGNTAGTFRYGIQAGLSFAGNDVVLRAGRLMDVAGNPALFPIYATLAYDRR
                     W"
     gene            complement(1433..2413)
                     /locus_tag="VFV99_07140"
     CDS             complement(1433..2413)
                     /locus_tag="VFV99_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5059117.1"
                     /translation="MRTIKPPLLVALTVSLAGCLCANDPGNLVPRTVAEDPSLPAIDL
                     NGTRLHSEAFGNPDAPVIIVMHGGPGSDYRAYLPLKALADDGLYVLFWDQRGTGLSER
                     LDPDAIDLDVYVEDLRLLIEHYAPHQPVVFVAKSWGAMYLTRFINQYGDYNGRIKGAV
                     LTEPGAFTDRQLSAYMARLQGSLNLVGEQFNDALWEGQFVSPADHERADYRAFQFALK
                     GAPSDHRDPNNLPPIWRPGAIVTAKMAKLAKQPFDWTTNLATFPNQVLFLRGDLNEAA
                     TLESQNEMAAAYPNARIETIAGVGHEVIWERTDEYLTHTRAYLREIGFLQ"
     gene            complement(2516..3670)
                     /locus_tag="VFV99_07145"
     CDS             complement(2516..3670)
                     /locus_tag="VFV99_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5059118.1"
                     /translation="MSGLEAGLLLGSVHSVVLAIALRRRVLNRRANQYLVALLVALAL
                     LLFDGFLRARGTLAAHPHLIGLTAWVPFVIGPLVFLYVREMTAPEPARLRPPWRHFII
                     PLAYIALLAVTFFPRSASYKRSIAAHEASWFVVALEAALLVYGIAYATAALVLLRRHR
                     EHVKELFSNLRGVSLRWLLVLALLNTMVWIAALVAFVIRMSGVVERGMASLIVPIGGT
                     ITAFVIGYFQLGQSEIFVPVKRDSVPPPSSPHEPVGEPATAEPTQSYARARLAEDDAT
                     ALESRIRAAMTEKQLYRRGGLTLGELADEVAATPHEVSQVLSTRLGRNFYTFVNEHRI
                     EHVKAELLRTDRPVLDIAFDAGFQSKSTFNSAFRKATGMTPSELRQRTTA"
     gene            complement(3690..4523)
                     /locus_tag="VFV99_07150"
     CDS             complement(3690..4523)
                     /locus_tag="VFV99_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059119.1"
                     /translation="MKTWIICALFLATASHVRAEGTEIDVRSFEFLDVIETADGNVWK
                     GVVIEQTPNVSYKIALAGGSIHVIPAADVQRMSKQRNGDFKNPRASAVREDGVEQSYE
                     PAAKLPAPYARSGLRVDPELSIVFPTGYYDHAGVQTSFAPGMRVGFESLFGNIGVSGG
                     GQARFTYWRLPGPTKDAAWLLETQLYGRAAVHIGRATPYIGLALGIDTNYVYSYALDD
                     SVTGVGLGMNLSTGLQLAVSPLVGVEIGGDYHPATDTISDMSEDSVSYFALRLGATVR
                     L"
     gene            4656..4919
                     /locus_tag="VFV99_07155"
     CDS             4656..4919
                     /locus_tag="VFV99_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059120.1"
                     /translation="MSAQLWKREGEEPRLTFRCPWCAVVGVSPVYGRHEQRAAIWLLA
                     GCPNVPCGRGVLIRVPAQAGWSQLDTGDDGMLLAPSCVYPHES"
     gene            complement(4916..5659)
                     /locus_tag="VFV99_07160"
     CDS             complement(4916..5659)
                     /locus_tag="VFV99_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5059121.1"
                     /translation="MRVVVLVALAACGRENFDLLDSPPTNPDADTRLPGLLAWYRMDD
                     EPSDGVLDDSSTNGRVARCIAGINCPTQVAGVRGMAAAFDGTQYARVSYGSWLATSAG
                     YSIAAWIYIDNDQFDQVAFAKPFGPSTSDAWGITTWSAAAGSGTCLETVDDAGGDQFV
                     CGPTLSAGRWFHVAGRWDGSTKALFIDAVKVGERTNAPASMIDTHDVVIGSDENAGMP
                     AYQFHGKVDELQIYDRALTDAEIAMLATP"
     gene            complement(5683..6075)
                     /locus_tag="VFV99_07165"
     CDS             complement(5683..6075)
                     /locus_tag="VFV99_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059122.1"
                     /translation="MLHAVEAPVLKLAAVAGAALLVGAFAIPRDQRVVQRLELHAPKR
                     AHAIYESAWNDGDIRITLPDGKPKPRVFYTRSFHWGCEWLSIETIRPDGENRYFYTYD
                     EEKLSCVEDAPPSWATPRVGYAYVVDTE"
     gene            complement(6144..7679)
                     /locus_tag="VFV99_07170"
     CDS             complement(6144..7679)
                     /locus_tag="VFV99_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5059123.1"
                     /translation="MHFWSVLAEQLWTARTPYVVVAYLLTRALINGTRVERYHLRAAA
                     TLVISHVVALAIGAGQTAFGYQSHVAETTSFAFEALAGMSIGVTMVFRALLPRVGFTL
                     PRILLDLLTAFGVIVVFIAVGRRAGFSVAGLITTSAVVTAVIGFSLQDTLGNVMGGLS
                     LQLDKSIAVGDWISLGPGQPSGQVTEIRWRYTAIELRTWDTVIIPNGMLVKSQITILG
                     RRTGAPQRTRRHIEFFVDFRWSPSEVIETVEAALQKDPVPRMALDPAPNVVMMGVRDS
                     YAQYIARYWLTDLAVDDQTDSAVRTRIWFALRRAGISLSIPATSIFLTHETPERETRK
                     AEREIEQRLRALKKVDLFSGLSAKLQRQLADQLELAPFARGEAITREGEQDDGLYMIV
                     EGDAVVQIGHADQSIRAVAQLGPGQFFGEMSLMTGEARTATVVAATDILCYRIQKAAF
                     QEILQETPAIADQIAEVLAMRRTALSAARDERDEVRKKRHETAKQDLLGRIRGFFGLD
                     ASR"
     gene            complement(7744..11502)
                     /locus_tag="VFV99_07175"
     CDS             complement(7744..11502)
                     /locus_tag="VFV99_07175"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5059124.1"
                     /translation="MRGLWPLALAFVYLSCANGHPGGTPDAGTGDEVDGSVDAAIDAP
                     PRYCGDGIKEIYEACDDGNTVDNDGCTNACTLPRCGDGIVQMNEGCDDGNMVDRDGCT
                     NHCALPTCGDGILQTGEECDDMNANETDACLSTCVTAKCGDGFVHTNVEQCDDMNSDN
                     TDNCITGCLLARCGDGYVHTNVEVCDDANNVDEGVCSPDCQMAGCPAGRAPCDSSPDN
                     GCETDITTSLGNCGGCNMQCTTPNATPVCNNGSCQIASCLTPYYDCNGLPVDGCEIDL
                     STDGANCGSCNHSCAAANTTTACVASACEVTGCLGTYADCTAVYGCETDISTSLGNCG
                     GCGQQCALANAGEACVSGTCSITTCDPGYADCNGLAADGCEVSVQSDLANCGSCGNQC
                     NLAHSAEACIAGACEIAACEGSYANCDGQTPDGCETNLATDGNNCGGCNHSCATANTT
                     TACVASSCEVTGCLGTYADCTAVYGCETNVATSMQNCGGCGQVCALANASETCAAGTC
                     NITACDAGFSNCNGADPDGCEISTASDVNNCGTCGNHCNLANAVEGCSAGACTITACD
                     GGYANCDGQTPDGCESNLATDGMNCGACGNSCATANSVTACVASSCQVLGCTGTYADC
                     TAAYGCETDTATSMQNCGGCGQQCALANASETCAAGSCNITACDAGFSNCNSNDADGC
                     EISTASDVNNCGICGNVCNLANAVEGCSAGACTIAACEGSYANCNGTTSDGCEINKGN
                     DALNCGACGNNCNNTYPNSVVGCSGGGCTFNGCQPSWWNLDGNLANGCEYSCTFVAGP
                     DLPDSNYTDSNCDGIDGDINAAIFVAINGNDSAPGTKTDPMQTIGAALTKAAVTGKTQ
                     VYVSNGTYNGRVTLRNGISIYGGYSRSNNWARSNLYVATIRSTTVPDGRITALDGTSI
                     SLPTTVALMTIDTVTTAPAGVSNYAVYCSSCSGLVLVNNVINAGNGGTGTAGGNGAFV
                     GNGSGGANGGGGSCDGSNWGAGGGGGTSACGRTGGTGGNGGPEGSNAGGNGTAGAGGT
                     PGGNGGSGGDPPSNGGNGQNGASGGTGAAGAGGSGGSTATNFWVSNNGGNGNGGGGGG
                     GGGGQGGFWVDDGSGNGGGGGGGGGCGGTAGDRGTGGGGSFGVFVVNSNGLLLSGNII
                     SSGNGGTGGNGGSGGGGGSGGNAGLGGTTCTGEVGRGGNGGFGGSGGSGGGGGGGAGG
                     PSYSVYRVGSGSVSTVSNALQFGGGGAGGVGGTPNGNNGATGASGGVF"
     gene            complement(11655..>12048)
                     /locus_tag="VFV99_07180"
     CDS             complement(11655..>12048)
                     /locus_tag="VFV99_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059125.1"
                     /translation="AADWLVDAALAAGDREMAGIAVRKLREAATTHKLKNIAAHAAFA
                     AGRFTLHAGDADAAAPELERAAELFTAANLPHEAARARLSLAQALAGKHAELAANEAK
                     TAATAFERLGAKRDASAAGELLRTLGRG"
BASE COUNT         1909 a         4106 c         3945 g         2088 t
ORIGIN      
        1 ctctacggcg agccggtgac gccgatcgtc gacgcgccgc cgcgcaatgt gctagctcaa
       61 gcacagtgat accgacgctg gtcgccgtcg tcgcgtgcct cgtactcgcc gtgctcagcg
      121 agctcgcgcg gcgtggcgca gctcgcgtgc tcggggcaac gccgaagcgt cgcgcgctcg
      181 cgatgtttgc cggctcgctc gcgatctacg ttgccatcgt cgtgatcgcg ttcgtgtact
      241 tccgcaccga aggtgttgcg accacgcaga tcgagtacgt cgtgcagagc gttcccgacg
      301 gatatcctgc gagcggcaag ctcgaggcgg gcgatcgcgt gatcgcgttc gacggtgagc
      361 cgatgtacct ctcgctcagc acgctggtcg acgagcgcaa cggcgctccg gtccgcctca
      421 cgttccgccg caacggcgcg acgcacgacg tgacgctgca gccggtcggc catgacggcc
      481 actgggtgct cgggttccgg ccgctcgccg atcacgcgcg cacgaccgat ggtgcgctcg
      541 ggcgcgcgct tggatttccg gtcacgcagc tcgaacagct cgtgccgccg ccggcgagcg
      601 accgcgccga tccgggcggt cccaagcgca tcatcgacat ccagcttgcg tcggagccat
      661 cgttcggcgt gaaggcgtcg cgtcagacgc tgctgctcgc gacgctattg ctcgtgctct
      721 cgctcgtcac cgacgtggtg cgcgcgattc gcacgctgcg cgcgagagcc tgaccgtcac
      781 caccgccggt cgtacgcgag cgtcgcgtag atcggaaaca gtgccggatt gccggcgaca
      841 tccatcaggc gtcccgcgcg caacacgacg tcattgccgg cgaacgacaa gccggcctgg
      901 atgccataac ggaacgtccc ggccgtgttg ccgtaccagc cgtccttcgc gtctgcgtaa
      961 acgaccatgc ggtatgtatc gctgtgcgtg atgtgcgtcg cgccggccca gtcgaagccg
     1021 acttcgccag cgacgaacca gcgaggcgcg tagtacccgc caagcacggc ggcatcgaca
     1081 ccgacattga tcattcgcgc ggcgtcgttg ttcgtgccgc gcactacgcc cgcgagactg
     1141 ccgatgagct gcagccggcg atgggtgacg atcggcgcgg acgcaccggc ttgcatgcgg
     1201 aagtcgtcga tgtcgatctc tgcccagtcg agctcgaggt cgccaccgag cacgagcaag
     1261 cggtcggcca cgctgatcgc gcgcgcgtag ccggccccga cgacaaatcc ctgttcggca
     1321 cccgctgtga gcgtcgcgac gtccgttccg tcttcgaccg cgccgtagtt gagatcctgt
     1381 gcgtgagcac tgatcggtgc ggcgatgagc gctgcgacca tgatgagctt cttcattgga
     1441 ggaatccgat ctcgcgcagg tatgcgcgcg tatgggtcag gtactcgtcg gtgcgttccc
     1501 agatcacttc gtggccaacg cccgcgatcg tttcgatccg tgcgttcgga tacgccgccg
     1561 ccatctcgtt ctggctctcg agcgtcgcgg cctcgttgag gtcgccgcgc aggaacagca
     1621 cctgattggg gaatgtcgcc aggttcgtcg tccagtcgaa cggctgcttc gcgagctttg
     1681 ccatcttcgc ggtgacaatc gcgccggggc gccagatcgg tggcaggttg ttcggatcac
     1741 gatggtcgga cggtgcgccc ttcaaggcga actggaacgc gcggtagtcg gcgcgttcgt
     1801 gatcggcggg cgacacgaat tggccctccc acagcgcatc gttgaactgc tcgccgacga
     1861 gattcagcga gccttgcagc cgcgccatgt acgcgctgag ctggcgatcg gtgaacgcgc
     1921 cgggctcggt caggactgcg cccttgatcc ggccgttgta gtcaccgtac tggttgatga
     1981 acctcgtcag gtacatcgcg ccccatgact tggcgacgaa caccaccggt tggtgtggcg
     2041 cgtagtgctc gatcaggagc cgcaggtcct cgacgtagac gtcgagatcg atcgcgtctg
     2101 gatcgagccg ctccgacaga cccgtcccgc gctggtccca gaacaggacg tacaagccat
     2161 cgtcggcgag cgccttcagc gggaggtacg cgcggtagtc cgagccggga ccgccgtgca
     2221 tgacgatgat gacgggtgca tccggattcc cgaaggcctc ggagtggagg cgcgtcccgt
     2281 tgaggtcgat cgcgggcagc gagggatcct cggcgaccgt cctgggcacg aggttgccgg
     2341 gatcgttcgc gcacaggcaa ccggcgagag agacggtgag ggcgactaag agaggaggtt
     2401 tgatcgttcg catgtccgca agctagagag cgcgagtgtc cacgtcgccc gggcccatag
     2461 gcccgcacgc gagcgcgtcc gacctgacgg gcccgcacgt ccgagccaag cagtttcagg
     2521 cagttgtgcg ctggcggagc tcgctcggcg tcatgccggt cgccttgcgg aaggcgctgt
     2581 tgaacgtcga cttcgactga aagccggcgt cgaacgcgat gtcgagcacg gggcgatcgg
     2641 tgcgcagaag ctcggccttg acgtgctcga tccggtgctc gttgacgaat gtgtagaagt
     2701 tgcgcccgag ccgcgtgctc agcacttgcg acacctcgtg cggggtcgcc gcgacctcgt
     2761 ccgcgagctc gccaagtgtg aggccaccgc gccggtacag ttgcttctcg gtcatcgccg
     2821 ccctgatccg cgattcgagc gcggtggcat cgtcctccgc gagtcgagcg cgtgcatacg
     2881 actgggtcgg ctcggctgtc gccggctcgc cgaccggctc gtgcggcgag ctcggcggcg
     2941 gaacggagtc gcgcttgacc ggcacgaaga tctcggactg gccgagctgg aagtagccaa
     3001 tcacgaacgc cgtgatcgtc cccccgattg gcacgatcaa cgacgccata ccgcgctcga
     3061 cgacgccgct catgcggatc acgaatgcca cgagcgccgc gatccagacc atcgtgttca
     3121 gcagtgcgag cacgagcagc cagcgcaaac tgacgccccg caggttcgag aacagttctt
     3181 tgacgtgctc gcgatgacgg cgcaagagca cgagcgccgc cgtcgcgtac gcgatgccgt
     3241 agacgagcaa tgcagcctcg agcgccacga caaaccacga tgcctcgtgc gcggcgatgc
     3301 ttcgcttgta gctcgcggag cgcgggaaga acgtgaccgc aagcagcgcg atgtatgcga
     3361 gcgggatgat gaagtggcgc cagggcggac ggagccgcgc cggctccggg gcggtcatct
     3421 cgcgcacgta gagaaacacg agcggcccga tcacgaacgg cacccatgcc gtgagtccga
     3481 tcaggtgcgg atgcgctgcg agcgtgcccc gcgcgcgcaa gaagccatcg aacaagagca
     3541 gcgcgagcgc gacgagcaac gccacgagat actggttcgc gcgccgattc aacacacggc
     3601 ggcgcagcgc gatcgcgagc accacgctgt gcacgctgcc gagaagcaga ccggcctcga
     3661 ggccactcat tcacggcaaa gctaactgat tacagccgca ccgtggcgcc gaggcgcagc
     3721 gcgaaatacg agacgctgtc ctcgctcatg tcggagatcg tgtcggtggc gggatggtag
     3781 tcgccaccga tctcgacgcc gacgagaggc gacaccgcga gctgcaaccc cgtcgacaga
     3841 ttcatgccga ggccaacgcc ggtcaccgaa tcgtcgagcg cgtacgagta gacgtagttc
     3901 gtgtcgatgc cgagcgcgag cccgatgtac ggcgtcgcgc ggccgatgtg caccgcggcg
     3961 cgcccgtaca gctgcgtctc gagcagccac gctgcgtcct tcgtcggccc gggcagacgc
     4021 cagtacgtga agcgcgcttg accaccgccg ctgacgccga tgttgccgaa caggctctcg
     4081 aatccgacgc gcatgcccgg tgcgaacgac gtctgcacgc ctgcgtgatc gtagtagccg
     4141 gtcggaaaca cgatcgacag ctcgggatcg acacgtaagc cagagcgtgc gtacggcgct
     4201 ggcagcttgg ccgccggttc gtagctctgc tcgacgccgt cctcgcgaac cgcgctcgcg
     4261 cgcggatttt tgaagtcgcc gttccgttgc ttcgacatcc gctggacgtc cgccgccgga
     4321 atcacgtgga tgctgccgcc ggcgagcgcg atcttgtagc tgacattcgg cgtctgctcg
     4381 atgacgacac ccttccagac gttgccgtcg gcagtctcga tcacgtcgag gaactcgaag
     4441 gagcgcacgt cgatctcggt tccttcagcg cgcacgtgcg aggcggtcgc gaggaacaat
     4501 gcacagatga tccaggtctt catgtcccgc taacccgcta gccgcaagaa gtcgcaagaa
     4561 atcgccggcc cccgcgcgct ggcagcgatc gcgacaaaac tgtcgcgtga tcacggttgc
     4621 ttccagctcg cgtgacctcg ggcacgatgc tggtcgtgtc tgcgcagctg tggaagcgag
     4681 aaggcgagga gcctcggctg acgttccggt gtccgtggtg cgccgtcgtc ggcgtctcgc
     4741 cggtctacgg ccgccacgag cagcgtgcgg cgatctggct gctcgccggc tgtccgaacg
     4801 tgccctgcgg tcgcggcgta ttgatccgcg tccccgcgca ggccggctgg tcgcagctcg
     4861 atactggcga cgacggcatg ctgctcgcgc cgagctgcgt ctatccgcac gagagttagg
     4921 gcgtggcgag catcgcgatc tcggcgtccg tcagcgcgcg atcgtaaatc tgcagctcgt
     4981 cgaccttgcc gtggaactga taggccggca tgcccgcatt ctcgtcagat ccgatcacga
     5041 cgtcgtgcgt gtcgatcatc gacgcggggg cgttcgtgcg ttcgccgact ttcaccgcgt
     5101 cgatgaacag cgccttcgtg cttccatccc atcggcccgc gacgtggaac caccggccgg
     5161 cgctcagcgt cggtccgcac acgaattggt caccgccagc atcgtcgact gtctcgaggc
     5221 acgtgccact gccggcggca gcagaccatg tcgtgatgcc ccacgcgtcc gatgtgctcg
     5281 gaccgaacgg cttggcgaac gcgacctggt cgaactgatc gttgtcgatg tagatccacg
     5341 ccgcgatcga gtagccggcc gatgtcgcga gccagctgcc atagctgacg cgtgcgtact
     5401 gcgtgccgtc gaatgcggcc gccattccgc gtacgccagc gacttgcgtc ggacagttga
     5461 tccccgcaat gcagcgtgcg acccggccgt tcgtgctgct gtcgtcgagc acaccatcgc
     5521 ttggctcatc gtccatgcgg taccaggcga gcaaccccgg cagccgtgtg tcggcgtcgg
     5581 ggttcgtcgg cggtgagtcg agcagatcga agttctctcg cccgcacgcg gcaagcgcga
     5641 ccaacacgac caccctcacc cgaatatctt acgccggaag tgctactcgg tgtcgacgac
     5701 gtacgcgtag ccgacgcgcg gcgtcgccca cgacggcggc gcatcttcga cgcacgacag
     5761 cttctcttcg tcgtaggtgt agaagtagcg gttctcgcca tcgggacgaa tcgtctcgat
     5821 cgatagccac tcgcagcccc agtggaacga tcgcgtatag aacacacgtg gcttcggctt
     5881 gccgtcgggg agcgtgatgc gaatgtcgcc atcgttccac gcgctctcgt agatcgcgtg
     5941 cgctcgcttc ggcgcgtgaa gctcgagccg ttgcacgact cgttgatcgc gcgggatcgc
     6001 gaacgcgccg accaagagcg ccgcccctgc aaccgccgcc aacttcagga caggagcctc
     6061 gacagcatgc agcatccttc aagcgtacgc gccgatcgta gacgcgccag cagttccttc
     6121 gggagcgcgt gcgagtgatg tgattaccgc gacgcgtcga gaccgaagaa gccacgaatc
     6181 cggccgagga gatcctgctt cgcggtctcg tgccgtttct tgcgtacctc gtcgcgttcg
     6241 tcgcgtgccg cgcttagcgc cgtgcgtcgc atcgccagga cctcggcgat ctgatccgca
     6301 attgcaggcg tctcctggag gatttcctgg aacgcggctt tctggatccg gtagcagagg
     6361 atgtcggtcg ccgcgacgac ggtggcggtg cgcgcttcgc cggtcatcag cgacatctcg
     6421 ccgaagaact gacccgggcc gagctgcgcg actgcccgaa tcgactgatc ggcgtgcccg
     6481 atctgcacga ccgcgtcgcc ctcgacgatc atgtacagcc cgtcgtcctg ctcgccctcg
     6541 cgcgtgatcg cttcgccgcg cgcaaacggt gcgagctcga gttggtccgc gagctggcgc
     6601 tgcagtttgg cgctgaggcc cgagaacaga tcgaccttct tgagcgcgcg caggcgctgc
     6661 tcgatctcgc gttcggcttt gcgcgtctcg cgctccggcg tctcgtgggt aagaaaaatg
     6721 ctggtggccg gaatcgacag cgagatgccg gcgcgccgca gcgcgaacca gatccgggtg
     6781 cgcacggcac tgtcggtctg atcgtcgacc gcgaggtcgg tgagccagta gcgcgcgatg
     6841 tactgcgcgt agctgtcgcg cacgcccatc atcacgacat tcggcgccgg atcgagtgcc
     6901 atgcgcggca ccggatcttt ctgcagcgcg gcctcgacgg tctcgatcac ctcgcttggt
     6961 gaccagcgaa agtcgacgaa gaattcgata tggcggcggg tccgctgcgg cgcgccggta
     7021 cgacggccga ggatcgtgat ctgactcttc acgagcatgc cgttgggaat gatcaccgtg
     7081 tcccacgtgc gcagctcgat cgcggtgtaa cgccaccgaa tctcggtgac ctgaccggat
     7141 ggttgaccgg ggccgagcga gatccagtcg ccgaccgcga tcgacttgtc gagctggagc
     7201 gacaagccgc ccatgacgtt gccgagcgtg tcctgcagcg agaagccgat cacggcggtg
     7261 acgaccgcgc tcgtcgtgat caaaccggcg accgaaaagc ccgcgcgccg accgactgcg
     7321 atgaacacga cgatcacacc gaacgcggtg agtagatcga gcaggatccg cggcagcgtg
     7381 aagccgacgc gcggcaacag tgcacggaag accatcgtga cgccgatcga catcccggcg
     7441 agcgcttcga acgcgaagct cgtggtctcc gcgacatggc tctggtagcc aaacgcggtc
     7501 tgcccggcgc cgatcgcgag cgcgacgacg tggctgatga ccagcgttgc agcggcacgc
     7561 aggtggtagc gctcgacacg cgtgccgttg atcagcgcgc gcgtcagcaa gtacgcgacg
     7621 acaacgtacg gcgtgcgtgc cgtccacagc tgttccgcga gcaccgacca gaaatgcacg
     7681 aaggtcgatg ctagccgatc gcggccgcct cctgccgcgt gcggcgtccg cgaactttac
     7741 cgattagaag acgccgccgg acgcaccggt cgcgccgttg ttgccgttcg gcgtgccgcc
     7801 aacaccgccg gccccaccgc cgccgaactg caaggcgttg ctcaccgtcg acaccgaccc
     7861 ggagccgacg cgatagacgc tgtacgacgg tccgcccgcg ccaccgccgc caccgccgcc
     7921 gctgccgccg ctgccgccga agccaccgtt gccgccgcga ccgacctcgc cggtgcacgt
     7981 cgtgccgccg agcccggcgt tgccgccgct gccgccacca ccgccgctgc cgccgttgcc
     8041 gccggtgccg ccatttcccg acgagatgat gttgccgctg agcaagaggc cattcgaatt
     8101 cacgacgaac acgccgaacg agcctccgcc gccggtgccg cgatcgcccg cggtgccgcc
     8161 gcagccgccg ccaccaccgc caccaccgcc attgccggaa ccgtcgtcga cccagaagcc
     8221 gccttgaccg ccccccccac caccaccgcc gccgccgttg ccgttgccgc cgttgttgct
     8281 gacccagaag ttggtcgcgg tgctgccgcc cgagccgccc gcgcccgcgg cacccgttcc
     8341 gccgctcgcg ccgttttgtc cgttgccgcc gttgctcggc gggtctccac cgctaccgcc
     8401 gttgccgccc ggagtgccgc cggctcccgc cgtgccgttg ccacctgcgt tcgaaccctc
     8461 tgggccgccg ttgccacccg taccaccagt gcggccgcac gcgctcgtgc caccgccacc
     8521 gccggcaccc cagttcgatc catcgcagct gccgccgccg ccgttcgcac cgccactgcc
     8581 gttgccgacg aacgcaccgt tgccaccggc cgtgccggtg ccaccgttgc cggcgttgat
     8641 cacgttgttc acgagtacga ggcccgagca gctcgagcag tacaccgcgt agttgctcac
     8701 gcccgccggc gccgtcgtca ccgtgtcgat cgtcatgagc gcgacggtgg tcggcagcga
     8761 gatcgacgtg ccatcgagcg cggtgatccg gccgtcggga accgtggtcg agcgaatcgt
     8821 cgcgacgtac aggttcgagc gcgcccagtt gttgctgcgc gaatagccac cgtagatcga
     8881 gatgccgtta cgcagcgtga cgcggccgtt gtacgtgccg ttcgacacgt agacttgcgt
     8941 cttgccggtc accgctgcct tggtcagcgc ggcgccgatc gtctgcatcg gatcggtttt
     9001 cgtgccgggc gccgagtcgt tgccgtttat cgcgacgaag atcgcggcgt tgatatcgcc
     9061 atcgatgccg tcgcagttgc tgtcggtata gttgctgtct ggtaggtcag gacccgcgac
     9121 gaacgtgcag gagtattcgc agccgttcgc gaggttgccg tcgaggttcc accagctcgg
     9181 ctggcagccg ttgaacgtac agccgccacc gctacagccg acgaccgagt tcggatacgt
     9241 gttgttgcag ttgttgccgc aggcgccgca gttgagcgcg tcgttgccct tgttgatctc
     9301 gcaaccgtcg gacgtcgtgc cattgcagtt cgcgtagctg ccctcgcacg cggcgatcgt
     9361 gcacgcgccg gccgagcagc cctcgaccgc gttcgcgaga ttgcagacgt ttccgcagat
     9421 gccgcagttg ttgacgtcgc tggcggtcga aatctcgcag ccatcggcgt cgttgctgtt
     9481 gcagttgctg aagcccgcgt cgcacgcggt gatattgcag ctgcctgccg cgcacgtctc
     9541 gctcgcgttc gcgagcgcgc actgctgacc gcacccgccg cagttctgca tgctcgtcgc
     9601 cgtatcggtc tcgcagccgt aggctgcggt gcagtcggca tacgtgccgg tgcagccgag
     9661 cacctggcat gacgacgcca cgcacgccgt gaccgagttc gcggtggcgc acgagttacc
     9721 gcacgcgccg cagttcatgc cgtcggtcgc gaggttggat tcacagccgt cgggtgtttg
     9781 cccgtcgcag ttcgcgtagc cgccgtcgca cgcggtgatc gtgcacgcgc cggccgagca
     9841 cccctcgacc gcattcgcga gattgcaatg attgccgcag gtaccgcagt tgttgacatc
     9901 gctggcggtc gagatctcgc agccgtccgg gtccgcgccg ttgcagttgc tgaaccccgc
     9961 gtcgcacgcg gtgatgttgc aggtgcctgc cgcgcacgtc tcgctcgcgt tggcgagcgc
    10021 gcacacctga ccgcacccgc cgcagttctg catgctcgtc gcgacattgg tctcgcagcc
    10081 gtacaccgcc gtgcagtccg cgtaggtgcc gaggcagccg gtgacttcgc acgacgatgc
    10141 gacgcatgcg gtcgtcgtgt tcgccgtggc gcacgagtga ttgcagccgc cgcagttgtt
    10201 gccgtcggtc gcaaggttgg tctcgcagcc atccggcgtt tgtccgtcgc agttcgcgta
    10261 gctgccctcg cacgcggcga tttcgcaggc gccggcaata cacgcctccg ccgagtgcgc
    10321 gaggttgcac tggttaccgc aactgccgca gttcgcgagg tcagactgca ccgagacctc
    10381 gcaaccgtcg gcggcgagcc cgttgcaatc ggcgtagccc gggtcgcacg tggtgatact
    10441 gcaggtgccg ctcacgcacg cttcgccggc gttcgcgagc gcgcactgct gaccgcagcc
    10501 gccgcagttc ccgaggctcg tcgagatatc agtctcgcag ccgtacaccg ccgtgcagtc
    10561 cgcataggtg ccgaggcagc cggtgacctc gcacgccgaa gcgacgcatg cggtggtcgt
    10621 gttcgcggcg gcgcacgagt ggttgcagct gccgcagttc gcgccgtcgg tcgacagatc
    10681 gatctcgcaa ccgtcgactg gcaggccgtt gcagtcgtag tacggcgtga ggcacgacgc
    10741 gatctggcag ctaccattgt tgcagaccgg cgtcgcgttc ggcgtcgtgc actgcatgtt
    10801 gcagccgccg cagttgccga gtgacgtggt gatgtcggtc tcgcagccat tgtccgggct
    10861 cgagtcgcat ggcgcgcgac ccgcggggca gccggccatc tggcagtcgg ggctgcacac
    10921 gccctcgtcc acattgttcg cgtcgtcgca gacctcgacg ttcgtgtgca cgtagccatc
    10981 accgcagcgc gcgagcagac agcccgtgat gcagttgtcg gtgttgtcgc tgttcatgtc
    11041 gtcgcactgc tcgacgttcg tgtgcacgaa tccgtcgccg cacttggcgg tcacgcacgt
    11101 cgacaagcac gcgtcggtct cgttcgcgtt catgtcatcg cactcttcgc cggtctgaag
    11161 aatgccgtcg ccgcacgtcg gcagcgcgca atggttcgtg cagccgtcgc gatcgaccat
    11221 gttgccgtcg tcgcagcctt cgttcatctg cacgatgccg tcaccgcagc gaggcagcgt
    11281 gcacgcgttc gtacagccgt cattgtcgac ggtgttgccg tcgtcgcacg cttcgtagat
    11341 ctctttgatg ccgtcgccgc agtagcgcgg cggtgcatcg atcgcagcgt cgaccgaacc
    11401 atcgacctcg tcgccggtcc ccgcatcagg cgttccacca ggatggccgt ttgcgcaact
    11461 cagatacacg aatgccaacg cgagcggcca cagtcccctc atgcgcgcga ctgtatcgat
    11521 cggcgatcgc gtcgccaatc gcaatctctc gacggcaacc cgaccgcgaa tacgacgccg
    11581 gctcaccatt gcaaaccgcc gtttgcgatc tcgggtggca atcgtcagct tgctgacgcg
    11641 acgaacatct cgggtcagcc gcggccgagc gtccgcaaca actcgccggc ggcggaggca
    11701 tcccgttttg caccgagccg ctcgaacgcg gtcgccgcgg tcttcgcctc gttcgcggca
    11761 agttccgcat gcttgcccgc gagcgcttgg gccaacgaca gccgagcgcg tgccgcttcg
    11821 tgcggcaggt tcgcggccgt gaacagctcg gccgcgcgct cgagctcggg ggctgccgcg
    11881 tcggcatccc cggcgtggag ggtgaagcga ccggcggcga aggcagcgtg cgcggcgatg
    11941 ttcttcagct tgtgggtggt ggcagcttcg cggagcttgc gcacggcgat cccggccatc
    12001 tcgcgatctc ctgccgcaag cgcagcgtcg acgagccaat ccgcagcc
//
LOCUS       DASUFX010000181         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_166736,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000181 DASUFX010000000
VERSION     DASUFX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166736"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFV99_07185"
     CDS             complement(<1..334)
                     /locus_tag="VFV99_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059126.1"
                     /translation="MKNLPGWAFGVLSVVGLVLIGSMALTWIDFGGMFSVRGYRLAID
                     DNHWLLLVPVAGLVLTLAASTRSPHARLAAIFAGLAVAGYVLFGLARSIIHSGLDTWL
                     MLGGAGMML"
     gene            complement(440..820)
                     /locus_tag="VFV99_07190"
     CDS             complement(440..820)
                     /locus_tag="VFV99_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEX5059127.1"
                     /translation="MKVLALALVLALAPTAAHAEKVFKKATANETWDCATDPVVRISQ
                     DKGTFGLLGECKRVTVSGKKNVVTIVSATKLTVSGTGNFVQVDDVGTIAVSGKGNQLT
                     WAKAKTGDKPKITKGKGNTVDQKK"
     gene            complement(871..2010)
                     /locus_tag="VFV99_07195"
     CDS             complement(871..2010)
                     /locus_tag="VFV99_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059128.1"
                     /translation="MALCACSISGPGPSQAPPETGATEPAGPDAETLPSAARLIPEPV
                     DSVEDLPGRYAQPFALPGQVSVVTLDAPNFGSGTYELYRSCGLPTCLHEVGQYHMVPT
                     NPAIGFAALSLVDQTGVVRSTYILDLLWRDGDGTLVAVQLRALIGDTTGPTQVWWRLP
                     DTASEPRSAPATDSSIDVSSPVALAAPSTNDNPPSFSGVFVRSLPILGDIGAITLDEA
                     AWYETTAVGTYNASYPYCLPWCFPETGGYELDLKNVKTGTGRLSLVPEGNPTQAHDYV
                     VYAIWRTVDGTPSAIQLQRIDGLVPSGPPFVMYRQWWNGQSAAPAPAAPSSCSVWLGL
                     AGYYRIRALQCGMSACWPLSGWYSSWAQYYQGLADAAGCSDESSP"
     gene            complement(2197..3138)
                     /locus_tag="VFV99_07200"
     CDS             complement(2197..3138)
                     /locus_tag="VFV99_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5059129.1"
                     /translation="MRRIVPSLVLVSACVTTDTHTIEQAIVGGTVANPADYPSVVALE
                     NTPGDWFCTGTLVSPEWVLTAAHCVDGQTAANTHVRFGDNDANSSAGGTVVSVTDVIG
                     YPGFDTNLWDNDVALIKLAQAVTNYTPTPIHREVVTPPAQVLEVGYGDTSETQGGGGI
                     LRQVTKTTVDCATANDASISGEHVICMNVGDGKGSCYGDSGGPLFETVAGARAVVGIT
                     SGGTDVCGRGFDIYTAVHDELAFIDATMGVMPPPDPMDPMDPGEGSGSGSGSGSGSGD
                     DDTGNGTTSAGCNATGSASSSLLVLLVALAFVRRRRF"
     gene            complement(3327..>4769)
                     /locus_tag="VFV99_07205"
     CDS             complement(3327..>4769)
                     /locus_tag="VFV99_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059130.1"
                     /translation="DDAPGAPLRRVVAADKLVLDAAQEHALAQLVTLLRGDAVIVIEG
                     PRGSGRSTAVARASGRPVVELDGARRERIDQQALLELRREVALGVAQPVIANADRLPA
                     ELGLGAFLDASRGSIVVTSAMPGLDLGTERPIVRVRWPLPEAPVRRTLWEQAAREPTG
                     DLDTLAFRYRVGAGVVHRAVASVRAEHGSRALDATDLMYGLRHNIAEDLGGLAHPVEV
                     TQSWEDLVLAEDTRDHVQALIARVRHAHQVLATWQYRSKIARGTGVPALFSGPPGTGK
                     TMVAGLIARELDLELYQVDLAQVVSKWVGETEKQLARVFDAAEQGHALLLFDEADALF
                     GKRSADMKGANDRYANLEVNYLLQRVEAFGGITILTTNLDTAIDPALKRRLAAHVVFE
                     LPDDDERTTLWQRNVTTRAAPLADGLDFEQLSRTFPKMSGANIRNAALAAAFLAAADG
                     TPTITQQHLLRAGRAEYRSMGHVLAERRAP"
BASE COUNT          796 a         1577 c         1587 g          809 t
ORIGIN      
        1 ccagcatcat gccggcgccg ccgagcatca gccacgtgtc gaggcccgag tggatgatcg
       61 agcgcgcgag gccaaacagc acgtagcccg caaccgcgag gcccgcgaag atcgccgcga
      121 gccgtgcatg cggcgagcgc gtcgatgccg cgagcgtgag cacgaggcct gcgacgggca
      181 cgagcaacag ccagtggttg tcgtcgatcg cgagccgata cccacgcacc gagaacatgc
      241 cgccgaaatc gatccacgtc agcgccatcg atccgatcag cacgagtccg acgacggaga
      301 gaacaccaaa cgcccagccc ggcaggttct tcatggcgcg cacggtaaag gagattggtc
      361 ggaccttcaa cagaaaccgg tcgacggggt gatttcagcc aacgtggtcg ccgtcgcatg
      421 atcggacgcg gggtcgccgt tacttcttct ggtcgaccgt gttccctttg cccttcgtga
      481 tcttcggctt gtcaccggtc tttgccttcg cccacgtgag ctgattgcct ttgccgctca
      541 ccgcgatcgt gccgacgtcg tcgacctgga cgaagttgcc agtgcccgac acggtgagct
      601 tcgtcgccga cacgatcgtg accacgttct tcttgccgct cacggtgacg cgtttgcact
      661 cgccgagcag cccgaacgtg cccttgtcct gactgatgcg gacaacggga tcggtcgcgc
      721 agtcccaggt ctcgttcgcc gtggccttct tgaagacctt ctccgcgtga gcggcggtcg
      781 gggcaagcgc gagaacgagc gcgagcgcga ggactttcat gccgcgagca taccgggttc
      841 cgaacgagcc catcgcgtca ggctaatgac tcagggtgac gactcgtccg agcagcccgc
      901 cgcgtcagcg aggccttgat agtactgcgc ccacgacgag taccagcctg acagcggcca
      961 gcacgcgctc atcccgcact gcaacgcgcg gatccggtag tagccggcga gtcccagcca
     1021 caccgagcag ctcgacggag cagccggcgc aggtgctgcg ctctgtccat tccaccactg
     1081 ccggtacatg acaaagggcg ggcccgacgg caccagacca tcgatgcgct ggagctggat
     1141 cgcagacggc gtgccgtcga cggtgcgcca gatcgcgtag acgacgtagt cgtgcgcttg
     1201 ggtcgggttg ccttcgggga ccaacgacaa ccggcccgtg ccggtcttga cgttcttcag
     1261 atcgagctcg tagccacccg tttccgggaa gcaccacggc aggcagtacg gatacgatgc
     1321 gttataggta ccgactgcgg tcgtctcgta ccaagccgct tcatcgagcg tgatcgcacc
     1381 gatgtcgccg agaatcggca acgagcgcac gaacacgccc gagaagctcg gcgggttatc
     1441 gttcgtgctc ggcgccgcga gagcgacagg cgatgacacg tcgatcgacg aatccgtcgc
     1501 gggtgcgctc ctgggctcgc tcgccgtgtc gggcagccgc caccagacct gcgtcggtcc
     1561 cgtcgtgtcg ccgatcagcg cgcgtagctg gacagcgacc agcgtgccgt cgccgtctcg
     1621 ccacaacagg tcgaggatgt acgtgctccg cacgacgccg gtctgatcga cgagcgacag
     1681 tgccgcaaac ccgatcgccg gattggtcgg caccatgtgg tactgcccga cctcgtgcag
     1741 gcacgtcggc agtccgcacg agcgatagag ctcgtaggtg ccactgccga agttcggcgc
     1801 atcgagcgtc accacgctca cctgccctgg caatgcgaac ggctgcgcgt agcggccggg
     1861 aagatcttcg accgagtcga ccggctccgg gatgagacgc gccgcggatg gcagcgtctc
     1921 ggcatcgggg ccagcaggtt ctgtcgcgcc ggtttcgggt ggcgcctggc tcggaccagg
     1981 gcccgaaatg ctgcaagcgc acaacgccaa agttgttacg attgttagta gcttattcat
     2041 tagtcggtct ccttttgctg cgccgcaaca tgagaccgtg gatccctcag gtcaagccgg
     2101 aaaaagtctt cagattccga aggcacgtcg aacagcggaa tgactcgcct attactttct
     2161 gtcctcggcg ttcgaacgtt tttcgcgcgc cggaacctag aagcgacggc ggcgcacgaa
     2221 cgcgagtgcg acaagcaaca cgagcaggct cgagctcgcc gagccggtgg cgttgcagcc
     2281 ggcgctagtg gtcccgttgc ctgtgtcgtc atcgcccgat cccgagccag acccggagcc
     2341 cgatcccgag ccctcgcctg gatccatcgg gtccatcggg tcgggtggcg gcatgacgcc
     2401 catcgtcgcg tcgatgaatg cgagctcgtc gtgaacggcg gtgtagatgt cgaacccgcg
     2461 cccgcacacg tcggtaccgc ccgaggtgat gccgacgacg gcgcgcgcgc ctgcgaccgt
     2521 ttcgaatagt ggcccgccgc tgtcgccgta gcagctgccc ttgccgtcgc cgacgttcat
     2581 gcagatcacg tgttcgcccg agatcgacgc gtcgttcgct gtcgcgcagt cgaccgtcgt
     2641 cttcgtgacc tggcgcaaga tgccgccgcc accttgggtt tcggacgtgt cgccgtaacc
     2701 gacctcgagg acctgcgccg gcggcgtgac cacctcgcgg tggatcggcg tcggcgtgta
     2761 gttggtgacc gcttgcgcga gcttgatgag cgcgacgtcg ttgtcccaga ggttcgtgtc
     2821 gaaaccggga tagccgatga cgtcggtcac cgagacgacc gtgccgcctg cggagctgtt
     2881 cgcatcgttg tcgccgaaac gaacatgggt gttcgccgcg gtctggccgt cgacgcagtg
     2941 cgcggcggtc agcacccatt cgggcgatac gagggttccg gtgcagaacc agtcgcccgg
     3001 ggtgttttcg agcgcgacga ccgacgggta gtcagccggg ttcgcgacgg taccgccgac
     3061 gatcgcttgc tcgatggtat gcgtgtcggt ggtgacgcac gcggagacga ggacaaggct
     3121 gggaacgatt cgccgcatgc gctgcccgtc gagcaagcgc cgcgccgatc gcgcaacgtg
     3181 agattcatgc gcaacggcgt gcgcgatttg gtgcgcgacg atgcattggc gtgtccgaag
     3241 ctgactactc gcggttacga cgacactccc agcgcgctgc gtcgtcgaga gtgagcgcgc
     3301 ctctgtcagt taactgcagt catcgatcac ggcgcgcggc gttcggcgag cacgtggccc
     3361 atcgaccggt actcggcgcg ccccgcacgc aacaggtgct gctgggtgat cgtcggcgtg
     3421 ccgtccgctg cggcgaggaa tgccgccgcg agcgccgcgt tgcggatgtt cgcgccactc
     3481 atcttcggga acgtgcgcga cagctgctcg aagtcgagac cgtcggcgag cggagccgcg
     3541 cgcgtcgtca cgttgcgttg ccatagcgtc gtgcgctcgt cgtcgtcggg cagctcgaac
     3601 acgacgtgtg cggcgagccg gcgcttgagc gcgggatcga tcgcggtgtc gaggttcgtt
     3661 gtcaggatcg tgatgccacc gaacgcctcg acgcgctgga gcaagtagtt gacctcgagg
     3721 ttcgcgtagc gatcgttggc gcctttcatg tccgccgaac gcttgccgaa cagcgcgtcg
     3781 gcctcgtcga acaagagcag cgcgtgacct tgctcggcgg cgtcgaacac gcgcgcgagc
     3841 tgcttctcgg tctcaccgac ccacttcgac acgacctgcg cgaggtcgac ttgatacagc
     3901 tcgagatcga gctcgcgtgc gatcagcccc gcgaccatcg tcttgccggt acccggtggt
     3961 cccgagaaca gtgccggcac accggtgcca cgcgcgatct tgctgcggta ctgccacgtc
     4021 gcgagtacct ggtgcgcgtg gcgcacgcgc gcgatcagcg cttgcacgtg atcgcgcgtg
     4081 tcctcggcga gcaccagatc ctcccacgac tgcgtgacct cgaccggatg cgcgaggcca
     4141 ccgagatcct cggcgatgtt gtggcgcaag ccgtacatca ggtcggttgc gtcgagcgca
     4201 cggctgccgt gctctgcacg caccgatgcc accgcgcgat gaacgacgcc ggcaccgaca
     4261 cgatagcgaa acgcgagcgt gtcgagatcg ccggtcggct cgcgtgccgc ctgctcccac
     4321 agcgtgcggc gcacaggtgc ttcgggaagt ggccagcgga cgcgtacgat cggccgttcg
     4381 gtgccgagat cgagcccggg catcgcggag gtaacgacga tgctgccgcg tgatgcgtcg
     4441 aggaatgcac cgagcccgag ctcggctggc aggcgatcgg cattcgcgat cacgggctgg
     4501 gcgacgccga gcgcgacctc acggcgcagc tcgagcagtg cttgctgatc gatgcgctcg
     4561 cggcgcgcac cgtcgagctc gacgaccggc cgaccactcg cgcgtgcgac cgcggtcgac
     4621 ctccccgacc ctcgcggccc ttcgatgacg atcacggcat cgccccgaag cagcgtgacg
     4681 agttgtgcga gcgcgtgctc ttgtgcggcg tcgagcacga gcttgtccgc agcgaccacg
     4741 cgacgcagtg gtgcgcccgg cgcatcgtc
//
LOCUS       DASUFX010000182         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_583900,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000182 DASUFX010000000
VERSION     DASUFX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583900"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFV99_07210"
     CDS             <1..356
                     /locus_tag="VFV99_07210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5059131.1"
                     /translation="AQPTTWIWEGVVMYLEQAAIEATLAVIQRRSASGSRIVVAYHAP
                     AWKLHLIGLVLKRVGEPLRSAFTPDAMRALLAKYEFRVVSDEDVPTIGSKLSPTLGRE
                     SRFLSHMRIVTADRG"
     gene            466..855
                     /locus_tag="VFV99_07215"
     CDS             466..855
                     /locus_tag="VFV99_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059132.1"
                     /translation="MTQALTTVDSNKRSVVVFPLRGDPWPTIATWAQRHKFMPREPQT
                     GNVKLFQKGSGFWTAAMRAQFTQNGDQMELQAWLPISIFARIAALFMLPAEMHIRSGG
                     FRAVLPRKIAREAINDLLAQVGATPIP"
     gene            complement(861..1295)
                     /locus_tag="VFV99_07220"
     CDS             complement(861..1295)
                     /locus_tag="VFV99_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5059133.1"
                     /translation="MPPVLVKHIMSAPVVTLFAEQTLPLAEDVMQFKHLRHLPVVDEH
                     QHLVGLVTHRDILRAQISSLTGLTDEQRRARQEDIKVSQIMTRDNIWTAGPETFASNA
                     GRLLLDHRFGCLPVLDTIGRLIGIVTERDYLRFAVKAIEMHD"
     gene            1448..1885
                     /locus_tag="VFV99_07225"
     CDS             1448..1885
                     /locus_tag="VFV99_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5059134.1"
                     /translation="MTAEDTRLLLLAQHDRLRQRSEACIRLAKLYETGEAWGAELDHA
                     LQELREEFATHNASETRVISELMHGPAAWGSLMIDRMLEEHLAEHAAFWELLSGTRAE
                     VAARIEELVDEIEAHMAAEERTFLAPVTLRDDVIRTRTREGSY"
     gene            complement(1886..4885)
                     /locus_tag="VFV99_07230"
     CDS             complement(1886..4885)
                     /locus_tag="VFV99_07230"
                     /inference="COORDINATES: protein motif:HMM:NF005898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5059135.1"
                     /translation="MRWAKRVRRLGLAWQNALEIARAGRLTAPYGAPFEVLHEERVYR
                     LRRYERAPDPGVTPIAEPLLLVPPLMVASEVYDISPDVSAVAFLARQGVDVWLVDFGA
                     PEREEGGMNRTLDDHVRAVSEAIGRVRTVTGKDVHLVGYSQGGMFAYQAAAFRKSEGL
                     ASIITMGSPVDLHRHLKVGEEITERLVAGMRAALAWPLSRLEGLPGMFTSTGFKLLSA
                     RKEALQLVDFVRNLHDRQALEKREAKRLFLGGEGFVAWPGPAFRKFVDDVIVGNRMAS
                     GGIVIDGHTITLADLTCPILYFVGNRDEMGRPGSVRGIRRAAPKVTKLYEVMLKAGHF
                     GLVVGSTALTVTWPGVVAWMRGEIPPSAIDVRAQAADREPVEVDDDEDDDDESPLDLA
                     KELVEKTAKAALDRVEELGEDLGTYLDNARWQLPRLHRLRNIDDDSRISLGKALADQA
                     ARIGDRTFFLWRGRAFTYADSNRRVDAIVRGLIATNVKVGTRVGVMMKSRPSHLSVVA
                     AVNRLGAVAVLLSPDTTDDVLPRALDLGEVEVLIVDPETAARVRAVTSIKVMVLGGVG
                     EQGELPPGMERPKRIIPPGVIDMETIDPDAVTLPAWYRPDAGRAHDLAMVFVTSGKYE
                     PPRAVRITNRRWAFSALGAAAAATLTTRDTVYCCLPLHHPSGSLVAAHSALIGGSRLA
                     LASRFAPETFWDEVRRYGVSVVYYAGEMCRRLVDADSVLGEKNNSVRLFVGSGMRADV
                     WRQLVDRFGPVGVLELYASTEANAVLANAAGKKIGSAGRPLPGSPEVAIAAYNFDDDD
                     LVHDGKGRLVRARLDEPGMLIARLSQRSADLAHIDPKRLLRDAFEPDDIWFVTGELFK
                     VDTMGDYWFVDRPSQMIHTRQGPVASTRIEDGLYDCPCVALCVAVPRPDPDEAGAQIP
                     VAAIQLHPNTSLDLAALATAVQALPEYARPRIIRVIDEIPLTDGFRPIKSRAFDRAGA
                     EYYWDPRTQSYEPLAPEPALRPVEAAR"
     gene            complement(4944..5375)
                     /locus_tag="VFV99_07235"
     CDS             complement(4944..5375)
                     /locus_tag="VFV99_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059136.1"
                     /translation="MNRAALALALSTAFLWGCATIVAGGPDTIPITTNPPGAYVYVNG
                     QVVGQTPMMVELDRSRSMADIRIYYPGFQPIQVMRYKTLNGWIFGNFFLAIFPVIVDF
                     ATDNWQRFDDEPIAIGLTPGQAPPPYGTQPMLPQQPMPPPR"
     gene            complement(5376..>5834)
                     /locus_tag="VFV99_07240"
     CDS             complement(5376..>5834)
                     /locus_tag="VFV99_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5059137.1"
                     /translation="TIRLGDVKFYFWPEALPKGEPPRGQGRTAPTRRDELVFSAKVTT
                     PRGHQLDLRQRVDGGVLRIGESSIELAAMEFGLLQLLVERRRSVADAELAYVAWHEIA
                     DALSFRSVQADSENVRELVHRVRRKLGSVQADDLIESKRGVGYRLAGEPG"
BASE COUNT          988 a         1909 c         1971 g          966 t
ORIGIN      
        1 cggcgcaacc gacgacgtgg atctgggaag gcgtcgtcat gtacctcgag caagcggcga
       61 tcgaagcgac actcgcggtg atccagcgcc ggtcggcgag cggcagtcga atcgttgtcg
      121 cgtatcacgc gccggcgtgg aagcttcatc tcatcgggct cgtgctcaag cgcgtcggcg
      181 agccgttgcg ctcggcgttc acgcccgacg cgatgcgtgc gctgctcgcg aagtacgagt
      241 ttcgagtcgt cagcgacgaa gacgttccga cgatcggcag caaactctcg ccgacgctcg
      301 gccgcgagtc gcgcttcttg agtcacatgc gcatcgtcac cgccgaccgc ggctagaacc
      361 cgcccttgcc gatgaacgcg acggcgggtt gcctgatctt ccggacgtat cctgcccgcc
      421 atgatctccc accctatacg gtcgcatggc gtagactccg tcgccatgac ccaggcactc
      481 acgaccgtgg actccaacaa gcgtagtgtt gtggtgttcc cgctccgcgg cgatccgtgg
      541 ccgaccatcg cgacgtgggc gcagcggcac aagttcatgc cgcgcgagcc gcagaccggt
      601 aacgtcaagc tgttccagaa gggcagcgga ttttggaccg cggcgatgcg tgcgcagttc
      661 acgcaaaacg gcgatcagat ggagctgcag gcgtggctgc cgatctcgat cttcgcgcgc
      721 atcgcagcgc tgttcatgct gccggccgag atgcacatcc gctcgggtgg tttccgcgcg
      781 gtgttgccgc gcaagatcgc gcgcgaagcg atcaacgatc tgctcgctca ggtcggcgcg
      841 acgccgattc cgtagcgcga tcagtcgtgc atctcgatcg ctttgaccgc gaatcgcaga
      901 tagtcgcgct cggtgacgat tccgatcagt cgaccgatcg tatcgagcac gggtaggcac
      961 ccgaagcggt gatcgagcaa gagcctgccg gcattggacg cgaacgtctc ggggccagcg
     1021 gtccagatgt tgtcgcgcgt catgatctgc gacaccttga tatcttcttg ccgcgcacgt
     1081 cgctgctcgt cggtgaggcc cgtcaacgag ctgatctgtg cacgcaggat gtcgcggtgc
     1141 gtgacgagcc cgacgagatg ttgatgctcg tcgacgaccg gtagatgccg caggtgtttg
     1201 aactgcatga catcctcggc gaggggcaac gtctgctccg cgaacagcgt cacgacgggc
     1261 gcgctcatga tgtgcttgac caggaccggc ggcatgcacc gaagcgttgc aagctcgtgc
     1321 ccaggtgtag tcgctcgatt tcgcgcagcc tctgcaccgc ccgaaggtcg actggcaact
     1381 cctgcgcaaa cctgacgagg tagcgccggg ttagctgctg gcccgcgact tgcgatgcct
     1441 ttgtccgatg acggccgaag acacacgtct actgttgctc gcccagcacg atcgtttgcg
     1501 tcagcgcagc gaagcctgca tccgccttgc gaagctctac gagaccggcg aggcgtgggg
     1561 cgccgagctc gaccacgcgc tccaggagtt gcgcgaggag ttcgcgacgc acaacgcgag
     1621 tgagacgcgg gtgatcagcg agctcatgca cggacctgcc gcatggggct cgctcatgat
     1681 cgaccgcatg ctcgaggagc atctcgccga gcacgcggcg ttctgggagc tcctctcggg
     1741 tacgcgtgcc gaggtcgcag cgcggatcga agagctcgtc gacgagatcg aagctcacat
     1801 ggccgcagag gaacggacgt ttctcgcacc ggtcacgttg cgcgatgacg tcattcggac
     1861 gcgcacgcgc gaaggcagct actgatcagc gagctgcttc gacgggccgg agcgcgggtt
     1921 ctggcgcgag cggctcgtag ctctgggtgc gcggatccca gtagtactcc gcacccgcgc
     1981 gatcgaatgc gcgcgacttg atcgggcgga agccgtcggt gagcggaatc tcgtcgataa
     2041 ctcggatgat tcgcggccgc gcgtactcgg gcagcgcttg caccgcggtc gcgagcgctg
     2101 cgagatcgag cgacgtgttt ggatgcagct ggatcgcagc gaccggaatc tgtgcgccgg
     2161 cctcgtcggg atcagggcgc gggacggcga cacacagcgc gacgcacggg cagtcgtaga
     2221 gaccgtcctc gatgcgcgtc gaggcgaccg ggccttggcg cgtatggatc atctggctcg
     2281 gccgatcgac gaaccagtag tcgcccatcg tgtcgacctt gaagagctca ccggtgacaa
     2341 accagatgtc gtcgggctcg aacgcgtcgc ggagtagacg cttcggatcg atgtgcgcga
     2401 ggtcagcgct acgttgcgac agccgggcga tcagcatgcc gggctcgtcg agccgcgcgc
     2461 gcacgagacg gcctttgccg tcgtgcacga gatcgtcgtc gtcgaagttg taggccgcaa
     2521 tcgcgacctc gggcgagccg ggcagcgggc gaccggccga accgatcttc ttgccggccg
     2581 cgttcgcgag cactgcgttc gcttccgtcg acgcgtagag ctcgagcaca ccgaccggac
     2641 cgaagcgatc gacgagctgg cgccagacat cggcacgcat gccggaaccg acgaacaacc
     2701 gcacggagtt gttcttctcg ccgagcaccg agtcggcgtc gacgaggcgg cggcacatct
     2761 cgccggcgta gtagacgacg ctgacaccgt agcgacgcac ttcgtcccag aacgtctcgg
     2821 gtgcgaagcg cgacgcgagc gcgagccgcg agccgccgat cagcgcgctg tgcgcagcga
     2881 cgagcgaacc tgacggatgg tggagcggca gacagcaata gacggtgtcg cgcgtcgtca
     2941 gcgtcgcggc ggctgcggcg ccgagcgccg aaaaggccca gcgccgattc gtgatgcgca
     3001 cggcgcgcgg cggttcgtac ttgccgctgg tgacgaacac catcgcgagg tcgtgcgcgc
     3061 ggccagcgtc gggccgatac cacgcgggca acgtgacggc gtcgggatcg atcgtctcca
     3121 tgtcgatcac gcccggcgga atgatgcgct tcgggcgctc catccccggc ggcaattcgc
     3181 cttgctcgcc gacgccgccg agcaccatca ccttgatgct agtgacagca cgcacgcgcg
     3241 cggcggtctc cggatcgacg atcagcacct cgacttcgcc gagatcgagc gcgcgcggca
     3301 ggacgtcgtc ggtggtgtcg ggtgacagca gcacggcgac ggcgccgagc cggttgacgg
     3361 cagcgacgac cgacaggtgc gacggccgtg acttcatcat cacgccgacg cgggtgccga
     3421 ccttgacgtt cgtcgcgatc aagccgcgca cgatcgcgtc gacgcgccgg ttcgagtcgg
     3481 cgtacgtgaa cgcgcggccg cgccacagaa agaacgtgcg atcgccgatg cgtgcggcct
     3541 gatcggcgag cgccttgccg agcgagatcc gcgaatcgtc gtcgatgttg cgcaggcgat
     3601 gcagccgcgg cagctgccag cgcgcgttgt cgagataggt accgagatcc tcgccgagct
     3661 cttcgacgcg atcgagcgcc gccttcgcgg tcttctcgac gagctccttt gcgagatcga
     3721 gcgggctctc gtcgtcgtcg tcctcgtcgt catcgacttc gaccggctct cgatcagctg
     3781 cctgcgcgcg cacgtcgatc gcgctcggcg gaatctcgcc gcgcatccac gccacgacac
     3841 cgggccacgt caccgtgagc gctgtcgagc cgacgacgag gccgaagtgc ccggccttga
     3901 gcatcacttc atacagcttc gtcaccttcg gtgcggcgcg ccgaatgccg cgcacggagc
     3961 ccgggcggcc catctcgtcg cggtttccga cgaagtagag aatggggcag gtgagatcgg
     4021 cgagcgtgat cgtatggccg tcgatcacga tgccgcccga cgccatgcgg ttgccgacga
     4081 tcacgtcgtc gacgaatttg cggaacgcag gacccggcca cgcgacgaat ccttcgccgc
     4141 cgaggaacag gcgcttcgct tcgcgctttt cgagcgcttg gcggtcgtgc aggttgcgca
     4201 cgaagtcgac gagctgcagc gcttccttgc gcgcggacag cagcttgaat cccgtcgacg
     4261 tgaacatgcc gggcagtcct tcgaggcgcg agagcggcca tgcgagcgcc gcgcgcatgc
     4321 ccgcgacgag ccgctccgtg atctcttcac cgaccttgag gtggcgatgt agatcgaccg
     4381 ggctgcccat cgtgatgatc gatgcgaggc cttcgctctt gcgaaacgca gcagcctggt
     4441 acgcgaacat gccgccctgc gagtagccga cgaggtggac atccttgccg gtgaccgtgc
     4501 gcacgcgacc gatcgcttcg gagaccgcgc gcacgtgatc gtcgagcgtg cggttcatgc
     4561 cgccttcttc acgctccggc gcaccgaaat cgaccagcca cacgtcgacg ccctggcgtg
     4621 cgaggaacgc gaccgcgctg acgtcgggag agatgtcgta gacctcggac gcgaccatca
     4681 gcggcggcac gagcagcagc ggctctgcga tcggcgtgac acccggatcc ggcgcgcgct
     4741 cgtagcggcg aagccgatag acgcgctctt cgtggagcac ttcgaacggc gcgccgtacg
     4801 gtgctgtcag gcgacccgcg cgcgcgatct cgagcgcgtt ctgccacgcg agcccgagcc
     4861 gacgtacgcg cttcgcccag cgcacttggc gacgacgtcg ttcggttgca ttcccgcgcc
     4921 tggactcgac catctcgtcg agtctatcgc ggcggcggca tcggctgttg cggcagcatc
     4981 ggttgcgtgc cgtatggcgg cggagcttga cccggcgtca gtccgatcgc gatcggttcg
     5041 tcgtcgaagc gctgccagtt atcagtcgcg aaatcgacga tgaccgggaa gatcgcgagg
     5101 aagaagttgc cgaagatcca gccgttcaac gtcttgtaac gcatgacctg aatcggctgg
     5161 aacccggggt agtagatgcg gatatccgcc atgctgcggc tgcggtcgag ctcgaccatc
     5221 atcggcgtct ggccgacgac ctggccgttg acatagacgt acgcccccgg cggattggtc
     5281 gtgatcggaa tcgtgtcggg accgccagcg acgatcgtcg cgcagcccca aagaaatgcg
     5341 gtgctcagtg cgagcgcgag tgccgcgcgg ttcacttagc cgggttctcc ggcgagccgg
     5401 tagccgacgc cgcgcttgct ttcgatcaag tcgtcggcct gcacgctgcc gagcttgcgg
     5461 cgcacgcggt gaacgagctc gcgcacgttc tcgctgtcgg cctgcaccga gcgaaacgac
     5521 agtgcgtccg caatctcgtg ccacgcgaca tacgcgagct cggcgtcggc gacagaccgg
     5581 cgccgctcga cgagcaattg caggaggcca aactccatcg cggcgagctc gatcgagctc
     5641 tcgccgatgc gcagtacgcc accgtccacg cgctggcgca gatcgagctg gtgaccgcgc
     5701 ggcgtggtga ccttcgcgga gaacacgagc tcgtcgcgac gcgtcggcgc agtgcggccc
     5761 tgaccgcgcg gcggctcgcc tttcgggagc gcctccggcc agaagtagaa cttcacgtca
     5821 ccgaggcgga tcgt
//
LOCUS       DASUFX010000183        13316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_166982,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000183 DASUFX010000000
VERSION     DASUFX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166982"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFV99_07245"
     CDS             <1..536
                     /locus_tag="VFV99_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059138.1"
                     /translation="IVDAGVTRARADARVAEPRADAAPRDASIAIDAVAMAVPPDAAV
                     ATTGFIVVKNDTWCEVTIDGDPRGRTGAMNASKPLRVDAGPHTVICEQPGTANKWTKQ
                     VEVGSGGSVTAAGSMLRAFAVTLGVDATIDGIPHKRGAVVTLKRGRHDFEINGVMTRF
                     DVSAACTVQSTPEPGCY"
     gene            660..1769
                     /locus_tag="VFV99_07250"
     CDS             660..1769
                     /locus_tag="VFV99_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059139.1"
                     /translation="MSRFALIGLVLSGCFYTDPINQRPSLDIVRLPPVADPYRGDMVS
                     LKANANDPEGYNVWFKWRAYACTDESDCDHAPYFEKSDERIDLMVPMDRDPTSTGAVR
                     AIHVFLEGVDDYGATARPIQQLWIGVADHAPTLELRAGSGYGNVVNTPINVYAKVGDQ
                     DDKPETPQLSWIVYTPTNNPAYEFVDITVPDDGDTKHKQYGKRITPHGTGKFTIEVTA
                     DDLLGSPTSTVTQTIELNVGDDRAPCLRQLTPLVATAPAALPMAEPTLFQVHVVEDDL
                     DPYPTINDTILGTTQFTWSLLAPGGSRQVLTGVTGNSVALDPASYQPGDILELRVEIA
                     DRVPRQIMCADSNATCSVISDNSCLQRQTWRVEVR"
     gene            1769..3538
                     /locus_tag="VFV99_07255"
     CDS             1769..3538
                     /locus_tag="VFV99_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059140.1"
                     /translation="MRKLVAIAMLSVAACSGDLSVGGDDNTTADGNTGMDAVPCGLQI
                     GFDPIDPVASSVLPIRAYTNLVNTAGVLSFTWTVTFNGANVPTTEQAADGSQINFIAA
                     TAGSYFVTVQVSGPTDCSYASTAKYVEAPGANADVFRLRTVPAPNLAPPQETYIQVKG
                     GANIDRAIALDSGISVTGLVKNTATSTGISAYLKFMPRSMPTAYTELFSSATGVYNLR
                     LLPYDHDVLVIPTATTLAPKLVPWSAVPFTTELHVGPGTVVSGTVHNPAGNGFAGAKV
                     QLYAGSVPSTIGTTAADGSFSVRTDFPTTSSQVTVKVTPPASSGLPRLEATSTFNLAN
                     ALNINYAASLATCNLQNTPVQRVGANQANAQVTVVGALGGVAGTIGGINATNTVRVSA
                     TADGTGRLPSMLVPRGALNVVTVLSMTDHAVSAIDTSACSVTQIDAPAMTTITGATKD
                     ATMTALPNIRIEAEPVGVLATAGVAPIHIMSNDTTGAFSLPLAAGGRYNVRFTDPQQR
                     AAPLTASNVAPGGVPTNAQLAKALALTGQVSIVNNSTPVIGASVQILCTTCSGLDLER
                     PIAETATNTVSRYAIAVPDPGTM"
     gene            3548..4369
                     /locus_tag="VFV99_07260"
     CDS             3548..4369
                     /locus_tag="VFV99_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059141.1"
                     /translation="MRRLLVLVCFLLFGARAYAETIAMLPLDGEKRLEIYGQPVAAEI
                     ARALKAAGLDVVVVGAKMDVPDRAQLIVDGTIKSTKTKQIELSIRVRDPRDGTVLDTL
                     PSPPTKLDDLDKAAADLSARVVPSVKTQLEKLVKPAVPNVPVEPTKPPPPKPIVPPQP
                     TLATAVTVRSKAPPLASLRDALDREAALFAAKQPYAGKLSLEVLTYKASDGQVPMARA
                     RVRAIIEGGTTKFDRIVRTDTIVGDPKKSTDDLAARTAREVLAILRPHLRRTLGH"
     gene            4369..5226
                     /locus_tag="VFV99_07265"
     CDS             4369..5226
                     /locus_tag="VFV99_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059142.1"
                     /translation="MQPRGTSSPGALVVLVLLAGTAVAQPSVEPSSDPALAPPSVEPS
                     SDPAIALREANAAATAGDWARVTATVQPLLARQLDRADLAEANRLAGIAAMFAQPPDH
                     AKAEQHFVAYLRIDIDGHLDPALYPPEVVNFFNDVRARHAAELKALRPKDRKRYAILS
                     VLPPFGQFQNGDKVKGIVIGSLLGAFAITNVTSYFVLRSWCFPQQGMGGSSTGCDGEM
                     VPEQNHNSSATTLRAVNITAGVGLILTYAYGVYDGIRGYRRDSRERALIPYATSSNGT
                     AMVGVHMNF"
     gene            5366..8299
                     /locus_tag="VFV99_07270"
     CDS             5366..8299
                     /locus_tag="VFV99_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059143.1"
                     /translation="MRQPVPFGKYLLLDRISVGGMAEVFKAKSYGVEGFEKIIAIKRI
                     LPTMGEDRDFIKMFIDEAKIAGQLAHANICQIFELGRIDGSHFIAMEYIWGKDLLQIQ
                     NRLRKTKQPMPISMASFVIAKVLEGLDYAHRKRDPLGRPLEIVHRDCSPQNVLVSYEG
                     EVKVIDFGIAKATSRNSRTMAGVLKGKFGYMSPEQVRGLPLDRRSDIFAIGTMLYECL
                     TGERLFQGETDFSTLEKVRNVDIAAPRSVNPAIPDAIEKIILKALAKDVEDRYQWCSE
                     MLADLQAYLMSQDVVFTAKSLSGWLKDVFSNEIERERQLLEQYKRVGRDGLIAGVPSA
                     QAKLDVVENLGEAGAPEGDATMLGGPNFDDVEKDTGAAPDVHQMPAAGGPRGSVQKKG
                     ASAPTPVGYQEDEEGEGDFGEEAPTEIFGETESPPPPVQQPAVGRSPSRPDIGGKAPP
                     KPPPGRPSTERPAVQPPMAARPASGGMGIQDGLAPTAGVGAYGQYAQPIGTPPGPQPA
                     MMGPPSGPQMAMGTPGAMEQQHPGAQGLAPHEGHQQPQRTVLGMQAPPGLGNYGSNYR
                     VSQQIPAQQPGMNPQVVQQNQSYPGYQQPYGYGQQGYPQQPQYGQYPSGQMPQMQMPM
                     GATPLDQGMPMAEGKRSTLVRDIGIGVGIAALVLVGFLVVKMFVLDNDSKSGGSGAQT
                     TSKFATVKLAMPAGVAAEMFVDNERVATVTSDSPGIPVSAGIKHIKLVGTNGLSCDDP
                     KVKLEAGKTTTLECNFGMTGSGGPATGSGTTATGSGETGTQVAQGGTAGSAETKATDT
                     KAVDPKATDTKATDTKAVDAKATDTKATDAKHVDTKAVDTKATDTKATDTKATDTKAT
                     DTKHTGDTKHVGDTKATDTKHADTKSTDPKSTDTKVTDTKATDTKAAAGDPNKGYLQV
                     FSKPPAKILVDGTDTNLKTPISGQSLSLAPGKHKITFVIGDDRFTYPVMIKAGTTETM
                     SKDLQ"
     gene            complement(8323..8700)
                     /locus_tag="VFV99_07275"
     CDS             complement(8323..8700)
                     /locus_tag="VFV99_07275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059144.1"
                     /translation="MIRYGEGVAAEDWFSLAQHVWPGAYGLAEVTAALARTSNIGAWD
                     GDTLIGAVRVLTDGHFLATVPEILVLPTYQRRGIGRELMRRALELAPRGKLFFGAQPQ
                     SVGFFERLGYERGPIGFVARRSP"
     gene            complement(8716..9348)
                     /locus_tag="VFV99_07280"
     CDS             complement(8716..9348)
                     /locus_tag="VFV99_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEX5059145.1"
                     /translation="MPETRIIKRYANRKLYDTEHSRYVTLDQISEMIRNGDDVKIVDN
                     KTKEDLTTVTLAQIIFEEEKKQRSFLPLNAMRNIIQSGGEWFAEAQRRVQSILPGKKK
                     EDEGGNGAPDDDSQAAAVADEVEGSPESEAAAKKRNLAALREWVDHSKHRLDEWQKQV
                     DSKVRGAIEDIQHAINPWASVNKDVKQLADRIAELEAKLHELEAHENKSS"
     gene            9473..10180
                     /locus_tag="VFV99_07285"
     CDS             9473..10180
                     /locus_tag="VFV99_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059146.1"
                     /translation="MLALVGALGEGSPDEWVQALSTIVTRAHLVDDADAIETLQALTH
                     AAAEPSLPYELRQRLYTAAVERNLPTIARLFLVASPQGELPHQLTKQLGPERPLRPTD
                     SRPLTLGERKALARTHRRDKLTLLIRDPHPQVVAIVLDNPHITEQDVVKMAAARPAVP
                     EALAKIAAHTRWSVRHAVKRALVLNPSTPLADAIRIATTLRAPELTELANDNSLPETL
                     RKHATEVLAELQTRPRA"
     gene            complement(10230..10508)
                     /gene="tatA"
                     /locus_tag="VFV99_07290"
     CDS             complement(10230..10508)
                     /gene="tatA"
                     /locus_tag="VFV99_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5059147.1"
                     /translation="MPGMGELVIILLIVLVVFGANKLPGIGDALGRSIKNFKKASSTD
                     DDKAVEDKKAAAELEAGKKAKELEAGKKDDDGEWEEVVVRRKKNKAEG"
     gene            10620..12605
                     /locus_tag="VFV99_07295"
     CDS             10620..12605
                     /locus_tag="VFV99_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5059148.1"
                     /translation="MRALVIAAICASTAHADPAVTAGPQPAPPVSAGEPKLNQELDGR
                     RNIRGCAVGESCARSADLLREFEAEAFPKPGASPWLDERTPVGSRIEAVTPRKVRRPS
                     ELRPDQAWLDKLELPDLPVTWTPRLIEYLLFYKNDPRGHSIMEAWLADQGRYRDLIVS
                     HLRKAKLPEDLLYVAMIESSYDVSTLSSAGALGLWQFMPEGGRIYGLRQDRWVDERRD
                     PLRATIAVVDYWKDLYQRFGDWQIAMGAFNVGYGAMLRSIARYNTNDYYQLCEYENGL
                     PWETCLYTPKVLATAIVGHNRAAFGFDKIKETPAETWDEVSVPASVSISVIARASGSD
                     ENVIKKLNPQLRKERTPPGETNYVVRVPRGARAEFGRKLAELSSDWDGMDAYVVAHGE
                     RLEDVATTFGISLNQLRKLNGIGRESEIEGGCVLVVPRISEDERAKNRAKAKANLHAS
                     GPDQKEGEPLIVAVPDKDLQIAGKKRVFYRVVSGDSITSVAKAMHVKTDQLVTWNTLD
                     PDAKLHPKMVLQAFVAPDFDAEREKVSLLDDAQLVIVTRGSDEHLDLAEERTGRVRTE
                     YISTGDEKLADVAKKYGMGSHDLARINRISYNTVLAKGQKIIVYQVADPSRSDRAEEQ
                     WKKMPKSKRSKTASQRNKSTSESDGPVTKPGQLEGDD"
     gene            12605..12967
                     /locus_tag="VFV99_07300"
     CDS             12605..12967
                     /locus_tag="VFV99_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059149.1"
                     /translation="MSMTRKEFLGTMLGAAGAVALVGCGGDDGASADAKARSCTMNGT
                     NVTIDANHGHVLMVSMADVMAGVDKTYDIMGTALHTHSVTVTAANFATLQNNPAGSVM
                     VTSSVSSAHSHGITILCA"
     gene            12993..>13316
                     /locus_tag="VFV99_07305"
     CDS             12993..>13316
                     /locus_tag="VFV99_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059150.1"
                     /translation="MTRLALAALLFAACGGDDGGGGGTTDGKMADAPSTNKVVTVTCP
                     TTPDREVSVNTAGTGYMPAATTAPVNAIVRFTMTATHDVAPNPQTTSDPGLRVGFGET
                     KCLKFT"
BASE COUNT         2485 a         4370 c         4300 g         2161 t
ORIGIN      
        1 tgatcgtcga tgcgggggtc acgcgtgcac gtgccgatgc gcgtgttgcc gagccacgtg
       61 ccgacgcggc gccacgcgat gcgagcatcg cgatcgatgc ggttgcgatg gcagtgccgc
      121 cagacgccgc cgtcgcgacg accgggttca tcgtcgtcaa gaacgacacc tggtgcgagg
      181 tcacgatcga tggcgatccg cgtggccgca cgggtgcgat gaatgcgtcg aagccgctgc
      241 gcgtcgatgc cggtccgcac accgtgatct gcgagcagcc cggcaccgcg aacaaatgga
      301 cgaagcaggt cgaggttggc agcggtggat cggtgacggc ggcgggctcg atgctccgcg
      361 cgttcgcggt cacgctcggc gtcgacgcga cgatcgacgg catcccgcac aagcggggcg
      421 ccgttgtgac cctcaagcgc ggccgccacg acttcgagat caacggggtc atgacccggt
      481 tcgatgtgag tgccgcctgc accgtccaaa gcacgcccga acccggctgc tattaggcac
      541 ttcggcggat ttctggaatc cgtgacatcg ctgtcgcgat cgtcgccgcg accccatgcg
      601 aggctctagt gattcgagta cttgcgggtg gcaggctggt tgcagcacaa cggaatctcg
      661 tgagtcgctt tgcgctcata ggactggtgt tgtcggggtg cttctacacc gacccgatca
      721 accagcgtcc atcgctcgac atcgtgcgtc tgccgccggt ggccgatccg taccgcggcg
      781 acatggtgag cctgaaggcg aacgcgaacg accccgaggg ctacaacgtc tggttcaagt
      841 ggcgggcgta cgcgtgcacc gacgagtccg actgcgacca cgcaccgtac ttcgagaagt
      901 cggacgagcg aatcgacttg atggtgccga tggatcgcga ccccacgtcg accggcgccg
      961 tgcgcgcgat ccacgtgttc ctcgaaggcg tcgatgatta cggtgcgacc gcgcgcccga
     1021 tccagcagct atggatcggc gttgccgatc acgcgccgac gctcgagctg cgcgcgggca
     1081 gcggttacgg caatgtcgtc aacacgccga tcaacgtcta cgcgaaggtc ggcgatcagg
     1141 acgataagcc tgagaccccg cagctgagct ggattgtgta cacgccgacg aacaatcccg
     1201 cgtacgagtt cgtcgacatc acggtcccag acgacggcga caccaagcac aagcaatacg
     1261 gcaaacgcat cacgccgcac ggcactggca agttcaccat cgaggtaact gcagacgact
     1321 tgcttggctc gccaacgtcg acggtgacgc agacgatcga gctcaacgtc ggcgatgacc
     1381 gtgcgccgtg cctgcgccag ctgacgccac tggtcgcgac ggcacctgcg gcgctgccga
     1441 tggcggagcc gaccttgttc caggtgcacg tcgtcgagga cgatctcgat ccgtacccga
     1501 cgatcaacga cacgatcctt ggcacgaccc agttcacgtg gtcgctgctc gccccaggcg
     1561 ggtcgcgcca ggtgctcacc ggtgtcacgg gtaatagcgt cgcgctcgat cctgcgagct
     1621 atcagccggg cgacattctc gagcttcgcg tcgagattgc cgatcgcgtt ccgaggcaga
     1681 tcatgtgtgc cgactcgaac gcgacatgct cggtgatctc ggacaacagt tgcctgcaac
     1741 gccagacctg gcgcgtggag gtgcggtgat gcgcaagcta gtcgcgatcg cgatgctgtc
     1801 cgtcgcggcg tgctcgggcg atctcagcgt cggcggtgat gacaacacga cggccgacgg
     1861 caatacgggc atggacgcag tgccgtgcgg cctgcagatc ggtttcgatc ccatcgatcc
     1921 ggtggcgagc tcggtgttgc caattcgtgc gtacacgaac ctcgtcaaca ccgccggcgt
     1981 gctgtcgttc acgtggacgg tcacgttcaa tggcgccaac gtgccgacga cggagcaggc
     2041 ggccgacggt tcgcagatca acttcatcgc ggccacagcc ggctcgtact tcgtcacggt
     2101 gcaggtcagt gggccgacgg actgctcgta cgcgtcgaca gcgaaatatg tcgaggcacc
     2161 cggtgccaac gcggacgtgt tccgcttgcg taccgtgcca gcaccgaacc tcgcgccgcc
     2221 acaggagacg tatatccagg tgaagggcgg cgcaaacatc gatcgcgcga tcgcactcga
     2281 cagtggcatc tcggtcactg gcctggtcaa gaacacggcg acgagcaccg gcatcagcgc
     2341 gtacctaaag ttcatgccgc gcagcatgcc gactgcgtac accgagctgt tctcgtcagc
     2401 gaccggtgtc tacaacctgc ggctcttgcc atacgaccac gacgtactcg tgattccgac
     2461 ggcgacgacg ctggcgccga agctcgtgcc gtggagcgcg gtgccattca cgacggagct
     2521 ccacgtcggg ccgggcaccg tcgtcagcgg caccgtgcac aaccccgccg gcaatggctt
     2581 cgcgggcgca aaagtgcagc tgtacgcggg cagcgtcccg tcgacgatcg gcacgacggc
     2641 ggccgacggc agcttctcgg tgcggaccga cttcccgacg acgtcgtcgc aagtgaccgt
     2701 caaagtcacg ccgcctgcgt cgagtggctt accgaggctc gaagcgacga gcacgttcaa
     2761 cctcgccaac gcactcaaca tcaactacgc ggcctcgctc gcgacgtgca atctacagaa
     2821 cacgcccgtg cagcgcgttg gtgcgaacca ggcgaacgcg caagtcacgg tcgtcggtgc
     2881 actcggcggc gtcgcgggta cgatcggcgg gatcaacgcg acgaacaccg tgcgcgtgag
     2941 cgcgaccgcc gacggcaccg gcagactgcc gtcgatgctg gtgccgcgcg gagcgctgaa
     3001 cgtggtcacc gtcttgtcga tgaccgatca cgcagtgagc gcgatcgaca ccagcgcgtg
     3061 cagcgtcacg cagatcgacg cgcccgcgat gacgacgatc accggtgcga cgaaggacgc
     3121 gacgatgact gcgttgccga acattcgcat cgaggccgag cccgtcggcg tgctcgccac
     3181 cgcgggcgtc gcgccgattc acatcatgtc gaacgacacg acgggcgcgt tctcgctgcc
     3241 gctcgctgct ggcggccgct acaacgtgcg gttcaccgat ccgcagcagc gcgccgcacc
     3301 gctcaccgcg agcaacgtcg cacccggcgg cgtgccgacg aacgcgcagc tcgccaaagc
     3361 actcgcgctc accggccagg tcagcatcgt caacaactcg accccggtga tcggcgcctc
     3421 ggtccagatc ctgtgcacca cgtgcagcgg gctcgacctc gagcgaccga tcgccgagac
     3481 cgcgacgaac accgtcagtc gctacgcgat cgcggtaccc gacccaggga cgatgtagga
     3541 tcggctcatg cgtcggctgc tggtgctcgt ttgcttcctg ctgttcggcg cacgtgcata
     3601 tgcagagacg atcgcgatgc tgccgctcga tggcgagaag cggctcgaga tctacggcca
     3661 gccggttgcc gccgagatcg cgcgtgcgct caaggctgcc ggcctcgacg tcgtcgtggt
     3721 cggcgcgaag atggacgtgc ctgatcgcgc gcagctgatc gtcgatggca cgatcaagtc
     3781 gacgaagacg aagcagatcg agctgtcgat ccgcgtccgc gatccgcgcg atggcaccgt
     3841 gctcgatacg ctgccgtcac cgccgacgaa gctcgacgat ctcgacaagg cggccgccga
     3901 tctctcggcg cgcgtggtgc cgtcggtgaa gacgcagctc gagaagctcg tgaagcccgc
     3961 cgtgccgaac gtgcccgtcg agccgacgaa accaccgccg ccgaagccca ttgtgccgcc
     4021 gcaacccacg ctcgcgaccg cggtgaccgt gcgctcgaag gcgccgccgc tcgcatcgct
     4081 gcgtgacgcg ctcgatcgcg aagccgcgct gttcgcagcg aagcagccct acgcaggcaa
     4141 gctctcgctc gaggtgctca cgtacaaggc gagcgacggc caggtgccga tggcgcgcgc
     4201 gcgtgtccgc gcgatcatcg agggcggtac gacgaagttc gatcgcatcg tgcgcaccga
     4261 caccatcgtc ggcgatccga agaagtcgac cgacgatctc gccgcacgta ccgcgcgcga
     4321 ggtgctcgcg atcctgcgtc ctcacctgag gcgaacgctg gggcactgat gcagcccagg
     4381 gggacttcct cgccgggtgc gctcgtcgtc ctcgtgctgc tcgcagggac cgctgtcgcg
     4441 cagccgtccg tcgagccgtc gagtgatcca gcgctcgcgc cgccctctgt cgagccgtcg
     4501 agcgatcccg cgatcgcgct tcgcgaagca aatgcagcgg cgaccgccgg cgattgggct
     4561 cgcgtgacgg cgaccgtcca acccctgctc gcacgccagc tcgaccgtgc ggacctcgcc
     4621 gaagcgaacc gcctcgccgg catcgccgcg atgttcgcgc agccccccga tcacgccaaa
     4681 gctgaacagc attttgtcgc gtatttacgt atcgacatcg acggccatct cgatcctgcg
     4741 ctgtacccgc ccgaggtcgt gaacttcttt aacgacgttc gcgcgcgcca tgccgccgag
     4801 ctcaaggcgc tgcgcccgaa ggaccgcaag cgctacgcga tcctgtcggt gctgccgccg
     4861 ttcggtcagt tccagaacgg cgacaaggtc aaaggcatcg tgatcggaag cctgctcggc
     4921 gcgttcgcga tcacgaacgt gacgagctac ttcgtgctgc gttcgtggtg tttcccccag
     4981 caaggaatgg gcggttcctc gacgggatgc gacggcgaaa tggtgccgga gcaaaatcac
     5041 aacagctcgg caactacact tcgtgcagtc aacatcacgg caggtgttgg tctcatcctc
     5101 acctacgcgt acggtgtgta cgacggtatc cgcggctatc ggcgcgactc acgcgagcgc
     5161 gcactgattc cgtacgccac gtcatcaaac ggcactgcga tggtgggcgt acacatgaat
     5221 ttctgaccgc gttcgggggc tacagcccct gggtttgcgt ccactgcacg agcgaacaga
     5281 cgcatactgt cgtcgcgccg ccgtggtttg ttggccgcgt tcggcgttgt gatctcccgt
     5341 tcgggcagat acgatttctt tcggagtgag gcagccggtc cccttcggga aatacttgct
     5401 cctcgatcgc atcagcgtcg ggggcatggc tgaggtcttc aaggccaagt cctacggcgt
     5461 cgagggtttc gagaagatca tcgcgatcaa acgaatcctg ccgacgatgg gtgaggaccg
     5521 cgacttcatc aagatgttca tcgatgaagc aaaaattgcg ggccagctcg cgcacgccaa
     5581 catctgccag atcttcgagc tcggccgcat cgacggctct cacttcatcg cgatggagta
     5641 catctggggc aaggacctgc tccagatcca aaaccggctg cgcaagacga agcagccgat
     5701 gccgatttcg atggcgagct tcgtcatcgc gaaggtgctc gagggtctcg actacgcgca
     5761 tcgcaagcga gacccgcttg gtcgtccgct cgagatcgtg caccgcgact gctcgccgca
     5821 gaacgtgctc gtctcgtacg agggcgaagt aaaggtcatc gacttcggca tcgcgaaggc
     5881 gacgagccgc aactcgcgca cgatggccgg cgtgctcaag ggcaagttcg gatacatgtc
     5941 gcccgagcag gtgcgcggtc tgccgctcga tcgccgcagc gacatcttcg cgatcggcac
     6001 gatgctctac gagtgtctga ccggtgagcg tctgttccaa ggcgagaccg atttctcgac
     6061 gctcgagaaa gtgcgcaacg tcgacatcgc cgcgccgcgc agcgtgaatc ctgcgattcc
     6121 ggacgcgatc gagaagatca tcctcaaggc gctcgcgaag gacgtcgagg atcgctacca
     6181 gtggtgcagc gagatgctcg ccgatctgca ggcgtacctg atgagtcagg acgtcgtgtt
     6241 cacggcgaag tcgctgtcgg gctggctcaa ggacgtcttc tcgaatgaga tcgagcgtga
     6301 gcgtcagctg ctcgagcaat acaagcgagt cggtcgcgat ggtctgatcg cgggtgtgcc
     6361 gtcggcgcaa gcgaagctcg atgtcgtcga gaatctcggc gaagcgggcg cgcccgaggg
     6421 cgacgcgacg atgctcggcg gccccaactt tgacgatgtc gaaaaagaca ccggtgcggc
     6481 gcccgacgtg caccagatgc ccgcggctgg ggggccgcgc ggttctgtgc agaagaaggg
     6541 ggcgtcggcg ccgacaccgg tgggctatca agaggacgag gaaggcgagg gcgatttcgg
     6601 cgaggaggct ccgacggaga tcttcggcga gaccgagtcg ccaccgccgc cggtgcagca
     6661 gcctgcggtc ggtcgctcgc cgtcgcgtcc tgacatcggt ggcaaagcgc cgccgaagcc
     6721 accgccgggc cgcccgagca ccgagcgtcc tgcggtgcag ccgccgatgg ccgcgcgtcc
     6781 cgcgagtggc ggcatgggca ttcaagatgg cctcgcgccg acggcaggcg tgggcgcgta
     6841 cggccagtac gcacagccga tcggaacgcc gcccggcccg cagcccgcga tgatgggtcc
     6901 gccgtccgga ccacagatgg cgatgggtac gcccggcgcg atggagcaac agcatccagg
     6961 cgcgcagggt ctcgcgccgc acgaaggtca tcagcagccg caacgcaccg tgctcggcat
     7021 gcaagcgccg ccgggcctcg gcaactacgg cagcaactat cgcgtgtcgc agcagatacc
     7081 cgcgcagcag ccgggcatga acccgcaagt ggttcagcag aaccagtcgt atcccggcta
     7141 ccagcagccc tatggctacg gccagcaggg gtacccgcag caaccgcagt acggccagta
     7201 tccgtcgggc cagatgccgc agatgcagat gccgatgggc gcgacgccgc ttgatcaagg
     7261 catgccgatg gcggagggca agcgctcgac gctggtccgc gatatcggca tcggcgtcgg
     7321 catcgctgcg ctcgtgctcg tcggtttcct cgtcgtgaag atgttcgtgc tcgacaacga
     7381 ctcgaagtcg ggcggctcgg gcgcgcagac gacgtcgaag tttgcgaccg tgaagctcgc
     7441 gatgccggct ggcgtcgccg cggagatgtt cgtcgacaac gagcgcgtcg cgacggtgac
     7501 cagcgactcg ccgggcatcc cggtcagcgc cggcatcaag cacatcaagc tcgtcggcac
     7561 gaacggcctg tcttgcgacg atccgaaggt gaagctcgag gccggcaaaa ccacgacgct
     7621 cgagtgcaac ttcggcatga ccggctcggg tggccccgca accggctcgg gcacaacggc
     7681 gaccggcagc ggcgagaccg gcacgcaggt ggcgcaaggc ggtacggctg gctccgccga
     7741 gaccaaggcg accgacacga aagccgtcga cccgaaggcg acggacacca aggcgacgga
     7801 caccaaagct gtcgacgcga aggcgacgga caccaaggcg accgacgcca agcacgtcga
     7861 cacgaaagcc gtcgatacga aggcgacgga caccaaggcg accgacacga aggcaaccga
     7921 caccaaggcg accgacacga agcacacggg cgacacgaag cacgtgggcg acaccaaggc
     7981 gaccgacacc aaacacgcgg acacgaagtc gaccgatccc aagtcgaccg acaccaaggt
     8041 gacggacacg aaggcgaccg acaccaaggc ggcagccgga gatccgaaca agggctacct
     8101 gcaggtgttc agcaagccgc cggcgaagat cctcgtcgac ggtaccgaca ccaatctcaa
     8161 gacgccgatc tctggccagt cgttgtcgct cgcgcccggc aagcacaaga tcacgttcgt
     8221 catcggcgat gaccgcttca cgtatccagt gatgatcaag gccggcacga ccgagacgat
     8281 gagcaaagac ctccagtagg tcgaaatggc gcgctgggtt cgctagggcg accggcgcgc
     8341 aacgaagccg ataggcccgc gctcgtaacc caagcgctcg aagaacccaa cactctgcgg
     8401 ctgcgccccg aagaacagct tgccgcgcgg tgctagctcg agcgcccgac gcatcagctc
     8461 gcggccgatt ccgcgccgct gataggtcgg caacaccagg atctccggaa ctgtagctag
     8521 gaagtgaccg tccgtgagga cccgaacggc tccgatcaac gtgtcgccgt cccaggcgcc
     8581 aatgttgctc gtgcgcgcta gtgcagctgt gacctcggca agcccgtaag cgccgggcca
     8641 gacgtgctgt gcgagcgaga accagtcctc cgccgcaacg ccttcaccgt agcggatcac
     8701 cttcgcggtg cctcgctacg acgacttgtt ctcgtgcgct tcgagctcgt gcagcttggc
     8761 ttcgagctcg gcgatgcgat ccgcgagctg cttgacgtct ttgttgacgc tcgcccacgg
     8821 attgatcgcg tgctgaatgt cttcgatcgc gccgcggact ttgctgtcca cctgcttctg
     8881 ccactcgtcg agtcgatgct tgctgtgatc gacccactcg cgtagcgcgg cgaggttccg
     8941 cttcttcgca gcggcttcgg actcgggcga accttcgact tcgtccgcaa cggcagcggc
     9001 ctgcgaatcg tcgtcggggg cgccgttgcc gccctcatct tccttcttct tgccgggcag
     9061 gatgctctgc actcggcgtt gcgcttccgc aaaccactcg ccaccgctct ggatgatgtt
     9121 gcgcatcgcg ttgagcggga ggaaggaacg ttgtttcttc tcttcctcga agatgatctg
     9181 cgcgagcgtg accgtcgtca gatcctcttt tgttttgttg tctacgattt ttacgtcatc
     9241 gccattccgg atcatttcgc tgatctggtc gagcgttacg taccgactgt gctcggtatc
     9301 gtagagcttt cgattcgcgt agcgctttat gatccgggtt tcgggcattg ggcgctccaa
     9361 tgattccaaa gttatcacgc accgcgccaa ggcctcaacg tgtcacactg aaagcgtggg
     9421 gccggaccac ataattgctc ggttaaaggc gatccgggac cctcgaatgc gggtgcttgc
     9481 attggtcgga gcactgggtg agggcagccc cgacgagtgg gtgcaggcgc tcagcaccat
     9541 cgttacccgg gcccacctcg tcgacgatgc cgacgccatc gagaccctcc aggcgctgac
     9601 ccacgcggcc gccgagccga gcctgcccta cgagctccgg cagcggcttt ataccgccgc
     9661 ggtcgagcgc aacctgccga ccatcgcgcg gctgttcctg gtcgcgtcgc cgcagggcga
     9721 gctgccgcac cagctgacca agcagctcgg ccccgagcgc ccgctacggc cgaccgactc
     9781 gcgcccgctc acgctcggcg agcggaaggc gctcgcgcgc acgcaccggc gcgacaagct
     9841 gacgctgctc attcgcgatc cgcacccgca agttgtcgcg atcgtcctcg acaacccgca
     9901 tatcaccgag caggacgtcg tgaagatggc ggcggcacgg cccgcagttc ccgaggcgct
     9961 ggcgaagatc gccgcgcaca cgcggtggtc cgtgcggcac gcagtcaaac gcgcgctcgt
    10021 gctgaatccg tcgacgccgc tcgccgacgc aattcggatc gcgacgacgc tgcgtgcacc
    10081 cgagctcacc gagctcgcga acgacaattc gttgcccgag acgctccgca agcacgccac
    10141 cgaggtgctc gccgagctgc agacgcggcc acgcgcataa agaccggcga gctggtgctc
    10201 gcggtttgaa gcccgaagtc ctaaggcggt tagccttcgg ccttgttctt cttgcggcgg
    10261 acgacgacct cttcccactc gccgtcgtcg tccttcttgc cggcctcgag ctccttggcc
    10321 ttcttgccgg cctcgagctc cgctgctgcc ttcttgtcct cgacagcctt gtcgtcgtcg
    10381 gtcgacgacg ccttcttgaa gttcttgata ctacggccga gcgcgtcgcc aatgcccggc
    10441 agcttgttgg ctccgaacac gacgagaacg atcagcagga tgatgactag ctcgcccatt
    10501 ccgggcatgg ggggctcctt cttagctgcc gcccaatgta gcaccgattc cgtcgacgct
    10561 cgaaaaactc cttgaaaact tggagacaag ttgtcgctcc gcctaatctt ccgttccgta
    10621 tgcgtgcgct agtcatcgcg gcaatttgcg cgagtacggc tcatgctgat ccggctgtta
    10681 cagcgggtcc gcaaccggca cccccggtca gcgcaggcga gcccaagctg aaccaggagc
    10741 tcgacggccg ccgaaatatt cgcggctgcg cggtcggcga atcttgtgcc cgcagcgccg
    10801 atctcctccg cgagttcgag gccgaggcgt ttccgaagcc gggcgcgagc ccctggctcg
    10861 acgagcgcac acccgtcggt agccgcatcg aagcggtcac gccgcgcaag gtaaggaggc
    10921 cgagcgagct gcggccggat caagcgtggc tcgacaagct cgagctcccc gacctgccgg
    10981 tgacatggac gccgcggctg atcgagtacc tgttgttcta caagaacgat ccacgtggcc
    11041 actcgatcat ggaggcgtgg ctcgccgatc agggtcgcta tcgcgatttg atcgtcagcc
    11101 atcttcgcaa ggcgaagctg cccgaggatt tgctctacgt cgcgatgatc gaatcgtcgt
    11161 acgacgtgtc gacgctgtcg tcggccggcg cgctcgggct ctggcagttc atgcccgagg
    11221 gcggccgcat ctacggcctg cgccaagatc gttgggtcga cgagcggcgc gatccgctgc
    11281 gcgcgacgat cgcggtcgtc gactactgga aggatctcta tcagcggttc ggcgactggc
    11341 agatcgcgat gggcgcgttc aacgtcggct acggcgcgat gctgcgctcg atcgctcgct
    11401 acaacacgaa cgactactac cagctgtgcg agtacgagaa cggtctgccg tgggagacgt
    11461 gcctgtacac gccgaaggtg ctcgcgaccg cgatcgtcgg tcacaaccgc gccgcgttcg
    11521 gcttcgacaa gatcaaggag acgccggcgg agacgtggga cgaggtgtcc gtgccggcga
    11581 gcgtgtcgat ctcggtgatc gcgcgcgcgt cgggcagtga cgagaacgtc atcaagaagc
    11641 tcaacccgca gctacgcaaa gagcgcacgc cgccgggcga gaccaactac gtcgtgcgcg
    11701 tgccacgggg cgcgagggcc gagttcggcc gcaagctcgc cgagctatcg agcgactggg
    11761 acggcatgga tgcgtacgtc gtcgcgcatg gcgagcgcct cgaggatgtc gcgaccacgt
    11821 tcggcatctc gctcaaccag ctgcgcaagc tcaacggcat cggtcgcgag agcgaaatcg
    11881 aaggcggctg cgtgctcgtc gtgccgcgga tcagcgaaga cgaacgcgcg aagaatcgcg
    11941 caaaggcgaa ggccaatctg catgcgtcag ggcccgatca aaaggaaggc gagccgctga
    12001 tcgtcgcggt gcctgacaag gacctccaga tcgccggcaa gaagcgcgtg ttctatcgcg
    12061 tcgtcagcgg tgactcgatc acgagtgtcg cgaaggcgat gcacgtcaag accgaccagc
    12121 tcgtcacctg gaacacgctc gatcccgacg caaagctgca tccgaagatg gtgctgcagg
    12181 cgtttgtcgc gccggacttc gacgccgagc gcgagaaggt atcgctgctc gacgatgcgc
    12241 agcttgtcat cgtcacgcgc ggctcggacg agcatctcga tcttgccgag gagcgcaccg
    12301 gccgcgtgcg taccgaatac atctcgacgg gtgacgagaa gctcgccgac gtcgcgaaga
    12361 agtacggcat gggctcgcac gacctcgcgc gcatcaaccg catttcctac aacacggtgc
    12421 tcgcgaaagg gcagaagatc atcgtctacc aggtcgctga tccgtcgcgc tctgaccgtg
    12481 cggaagagca gtggaagaag atgccgaagt cgaagcgatc gaagaccgcg tcgcagcgga
    12541 acaagagcac gtcggagtcg gacggtccgg tcacgaaacc gggacaactc gaaggagacg
    12601 actaatgagc atgacccgca aagaatttct cggcacgatg ctcggcgcgg ccggcgcggt
    12661 agcgctcgtg ggctgcggtg gtgatgacgg cgccagtgca gacgcgaagg cgaggagctg
    12721 cacgatgaac ggcacgaacg tcacgatcga cgcgaaccac ggccacgtgc tgatggtctc
    12781 gatggccgac gtcatggccg gcgtcgacaa gacatacgac atcatgggca ccgcgcttca
    12841 cacgcactcg gtgacggtca cggcggcgaa tttcgccacg ctgcagaaca acccggctgg
    12901 ctcggtgatg gtgacgtcga gcgtcagcag cgcgcactcg cacggaatca cgattctctg
    12961 cgcctgatgt tatcgtcgcg gcggaggtag caatgactcg actggctcta gctgcactgc
    13021 tgttcgcagc ctgtggcggc gacgatggtg gcggcggtgg caccacggac gggaagatgg
    13081 ccgatgcacc gtcgacgaac aaggtcgtca cggtgacgtg cccgacaacc cctgaccgcg
    13141 aggtcagcgt caacacggcc ggcacgggtt acatgccggc ggcgacgacc gcgccagtca
    13201 atgcgatcgt cagattcacg atgaccgcga cgcatgacgt ggcgccgaac ccacaaacga
    13261 cgagcgatcc gggcctgcgc gtcggcttcg gcgagaccaa gtgcctcaag ttcacg
//
LOCUS       DASUFX010000184        16014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1001715, whole genome shotgun sequence.
ACCESSION   DASUFX010000184 DASUFX010000000
VERSION     DASUFX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16014
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001715"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFV99_07310"
     CDS             complement(<1..527)
                     /locus_tag="VFV99_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5059151.1"
                     /translation="MVDDDAANELQIVIGADEAGGRLDAVIAAAIESLSRAQVQRLIE
                     DGQVQVNGVTAAKSARVRDGDVIDVRVPAPEPLEVLPEPIPLAILFEDPDLIVVNKPA
                     GLVVHPAAGHASGTLVNALLFHCKDLSGIGGVLRPGIVHRLDKDTSGVMVATKSERAH
                     AALTAAFAAKSRGEQG"
     gene            complement(521..787)
                     /locus_tag="VFV99_07315"
     CDS             complement(521..787)
                     /locus_tag="VFV99_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059152.1"
                     /translation="MATLIDNPKRARQLARAIASDLTLYHEPKILEGIANDNLFEVMS
                     DEINEGRELFKSRVTEDIFGQHIYERAIVDVLVKSKGHVKSKIW"
     gene            complement(820..2406)
                     /locus_tag="VFV99_07320"
     CDS             complement(820..2406)
                     /locus_tag="VFV99_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5059153.1"
                     /translation="MTPRVVAIGGGKGGVGKSLVAANVGIFLATLGKKVVLLDGAFGA
                     PNLHIFAGVPRPSRSLAEALVPGGPPLTELAVSTHVPGVKLIGGVYDPPWIANPDADQ
                     VRVIADQLHELPADWVVVDLGPGLGAATLELFLEADIQLLIGVPDPTSIELMHRFVRA
                     AFLKRLERKGFSHLAQIAGKQPNDFEGGTPSALDVYLGAVAAQHSELGELRDAILGFA
                     PHLVINSARSKSDMELGRAVASAARRRLGTPLRYLGHLEYDEAVWASTRRRRPLLIEH
                     PETRIAKCFERVARGLLAVKPVGRSDSGGFPTNTFDTDVLPSDSHYELLEVPPTASFE
                     DIRRANRRIRDIYGAESIAISGLYDPVSLEAVHRRLDLAYTTLMDAAKRKEYDLELFP
                     DGVPMPITPVERLADVSAPRPAAKVDDPATLTIRPPMPEISPRTEYSGPLLRQIREAV
                     GVELREIAEKSKIGMAYLQALEGEVFAKLPAPVYVRGFLAEYARALGLDAERVKQTYL
                     ERYRAARGPVIEAEDEAAKP"
     gene            complement(2451..3626)
                     /locus_tag="VFV99_07325"
     CDS             complement(2451..3626)
                     /locus_tag="VFV99_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5059154.1"
                     /translation="MKEQNMRIKNVIVFACLVACGDGDKPATEGPCDVADGQCIFRHD
                     TFGDEQLWTDTLRLHELVQNLKPTTALSVGLKVDADAVPPDVLASADLENPATTVALI
                     GLNAVVGVRGEVENGTITKIGITCALCHSTVDNSVTAGIGKRLDGWPNRDLNPGAIVS
                     LTPGLDALTAKLGVDNAVAKAALESWGAGKYDARFNQDTESHPVLIPPAYGLRDVALE
                     TYTGEGPVSYWNAYVAVTQMGGHGSFVDDRIGVAIHQEPDRVTPMLPALREYQFSFDP
                     PAPPAGSFDAAAAARGKTLFDGQARCASCHSGSSFTDAPTLHAATETGMATDEARRSA
                     TGKYRTTPLRGVWQHPPYFHDGSAATLLDVVGHYNTVLGLSLTAEQQSDLEQYLRSL"
     gene            complement(3702..5066)
                     /gene="selA"
                     /locus_tag="VFV99_07330"
     CDS             complement(3702..5066)
                     /gene="selA"
                     /locus_tag="VFV99_07330"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX5059155.1"
                     /translation="MSDPRLAKLPAVHALADEAEALGSLPRWAVVEAARRAIAARRTA
                     ILAGNADSSSIAALEVAELAATLARPALRRVINATGVVLHTNLGRAPLADSARAAIDE
                     VARGYSNLEYDLDKGERGSRHDHLRALIRELTGAEDAIVCNNNAAATVLGLAALATNK
                     EIVVSRGELIEIGGSFRLPEILALSRGTLVEIGTTNKTHKRDYENAIGPNTGLLLKVH
                     RSNFAIVGFTAEVSPEEVVAIGRAKGIATMIDLGSGMFVDRATQRSWHLPDEPTVAEC
                     VASGADLVTFSGDKLLGGPQAGILVGKTAAVETARKHPLMRALRPDKLTIAALAATLA
                     HYRDANLDEIPTVRMIGRTSEALHADAQRLREELGRLAGLTVTVEGCMSTVGGGAMPT
                     AQLPSWALVISGRSAEALDRKLRAARVPVIARIEDGKLWLDVRTLAETDYVELIEALR
                     SLAT"
     gene            complement(5336..5566)
                     /locus_tag="VFV99_07335"
     CDS             complement(5336..5566)
                     /locus_tag="VFV99_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059156.1"
                     /translation="MRAVVGRAPVSPRAYVPDSETVIGPRHYSRGYPAQNDDGSVNAV
                     IEIPTGTTGKFEVDEETGWLRWQHAREGGSAT"
     gene            5818..8079
                     /locus_tag="VFV99_07340"
     CDS             5818..8079
                     /locus_tag="VFV99_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059157.1"
                     /translation="MDVQAVSALLAALLILAIGTSVLLRSRSDKMYTTFAAMTFTVSA
                     WHLCSFIAAATGSPVMGWLSLWAAATIPPTAIRFFRVFLAQPSIGGPKRGPRVTLAWT
                     LLAYAALVYSAIVQPIHETTWFLVPFGMYVFGGLYRCVWDMFVQYRATSKRVERTRVG
                     YLTLGGFVATTLTLTDVLPKLGVAWPAIGNVLSILYLYFLSQTLFRYRLLDLNELVGK
                     MAVLGTLVALLSAVYGFLLYWVRGQSTQGLLLLNALVAAFVILILFEPVRNWLENSIN
                     RWLLRQRTELRGRLEAVRRELPGVVDVQDMVARIMTALEESRRVTEAGVYLLDADGAG
                     FDCAGSLGKTPPERLDANAERALLDRVRVGYLDLDALTRELDDLGQAPDVEAKRTALV
                     SLKTRVEELQATLIFPLFGSAETEQGPWLLGLFCVRDDRTESAFDPDDIDTFRQLAIG
                     AARVIESSQAYERVKERDRLAALGEMAAGLAHEIRNPLGAIKGAAQLLMTSDGPQQHH
                     TENAEFLQIIVEEANRLNNVVTRFLDYARAERPGTEGADKVDLNNVVRKTVQLLQKEQ
                     LKNIEVRVRTDEQLPQVAGDPESLLQVFLNLGQNAIQAMPDGGTLEILTTRRRRSRLG
                     YGQFCEIRFRDTGIGIPRDRLKKLFIPFYTTKQKGTGLGLAISHRIINQHGGTIEVRS
                     TIGQGSTFSVFLPAAEPMGKIEDITETGNLKTLPDKPEKPEKPEKSEKPETPADEPVV
                     PAAAPATSSTGTG"
     gene            8092..9531
                     /locus_tag="VFV99_07345"
     CDS             8092..9531
                     /locus_tag="VFV99_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5059158.1"
                     /translation="MQPAKILVADDEQNLRRVLTALLRREGHDVIQAASGLEAIDRLA
                     DVDVVITDLRMPGADGMEVLRTASKNHPQVPVIMITAYGSVGQAVEAIKAGAFDYIEK
                     PFEQDAIRIIIDKAIGQAAANKLAPQQQLYLSSESEQRGKFGLVGTSAEMQGIFAVIE
                     KVADTPSTVLITGESGTGKELVAKALHEQSSRKGGPFIKINCAAIPKTLMESELFGYE
                     KGAFTGATSSKPGRFELADGGTLFLDEIGEIPVEMQVKLLRAIQESEFERVGGLKTIK
                     VDVRLITATNRDLEHEIARGNFREDLFYRLNVVPLQIPPLRKRTGDIPLLVQHVIKKF
                     NERLKKNVTGMSDEALAALESHNWPGNIRELENVLERTILFCKGDRIERADLQLVLAP
                     SNQASNQSGSMAPIEAPLEDEIDEHAEISGSLKEVVRAETARVERELIVKALDETGGN
                     VTQAARLLKISRKSLQMKMKEFGLRDREG"
     gene            complement(9567..9992)
                     /locus_tag="VFV99_07350"
     CDS             complement(9567..9992)
                     /locus_tag="VFV99_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059159.1"
                     /translation="MTPYPLTLQLGMKRFHGHVFLAPNRLYFVCTKTGGAWAAAIGQG
                     LGGAIGGAIAAGFQPTAGAAPTVVDEGQLQQAVAQNEGSMIMDASKIEEIKETIWWRL
                     IRFDGKKFGLPRGLGKPLKQELGKWAAAHNVKTKGLPTA"
     gene            10028..11350
                     /locus_tag="VFV99_07355"
     CDS             10028..11350
                     /locus_tag="VFV99_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059160.1"
                     /translation="MLRPVSVLVVLGLSGSAFANPNSIVPGGQDPDYPDAGFAQKGVD
                     VYGEIEYEYELDSSKIVRERVGDPAADPLDGVTTVRDLDFKQYKHTLTPRLYVGVFRD
                     TFVYGALPIVITQARELSFINPSRASSTVNDGLLPMQGFDARDPGTPTPGDLAFRGPD
                     RHGLDQVHFGLGTALMNQLKDDTKPTWKLGAEVRLAIGTIMKFDAMAPEANKGVSKGV
                     QELKLWTTFARKLGWAEPWVEMYWQVPLSSKSGSLFDDPGFGATNVKPSQQAGIASGL
                     EIYALDNRADQTRISFDIGSKVDAHFEGREYTEMWEVFAAAGDPRSMGPLVLDADPTR
                     SGVQALSHPGISNIENYLEMTGRFALRAQIGPHVRFGVIADLIWKTDHVISFADAGVD
                     REDDNDLVNPGTDEVNPLHTPVIDLVGHRFRAEDAFDINIGVSGTVLF"
     gene            11444..13582
                     /locus_tag="VFV99_07360"
     CDS             11444..13582
                     /locus_tag="VFV99_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059161.1"
                     /translation="MLARRIIAVSPDKSFGKQLATALKAAGGAVDLHLKLDDMAKGEI
                     VAALVVLHLDGELATAAAEILPRLSSDAKVIAIIPKSNLAAIVDIMQSSDKIAGMLMA
                     ENFDSRQLSAMATRVIAGDIFGLEKNISWGTLVHNQLVGDYQEKSLCISQISEFAELM
                     GVRRKYREAIEQCIDEMLMNALYDAPVDEQGKPLFSEIPTKTRISLRVEQKVVVQYAC
                     DGKQFAVSVRDAFGTLERNTVLRYLYKCLHSEQQIDRKVGGAGLGLYLMVNSATTVFF
                     NVLTGVATEAVCVFDLETPKLQLERFGFFTEKIDAGGRLAAGTSRRLPQGHPVERRRS
                     PAPPPPAPRGLIAALSVAILAVLVLVGIAAWPRLFPATQTAKVTFTTIPKGATIEIEG
                     RNEGTATEGQLERELEVGRAYPVVARLDGYEPKTSVVQPQRGGSHVTFELVARTATVF
                     LDTQPTGATIEVDGKSLGTTPLSITTLPPGTSVQYTIKKTGYHPATGTLDVPGPGKEV
                     RMVMPLAVSDELARIKLTSDPAGAQVYQNGQLLAGVQTPAEVLVEAGKVQRFMLTMPN
                     MVPAFIEPFTPARGAVGVEKKARLVAGQPLRIEATNDGKVTVTPAQHCKELTPPAECV
                     LAPGTYTVDYNVPPSKFSHQVTVGTKPATDKFDLGFVEAAEGKHIMIGGKQVKKALFE
                     AGSRQVTIADDAGTRTVSVKVKAGATVTAK"
     gene            13661..14944
                     /locus_tag="VFV99_07365"
     CDS             13661..14944
                     /locus_tag="VFV99_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX5059162.1"
                     /translation="MKVAVAALLVLAAEASAAPSLTSSSDPYPGIHREIWRDTDPQQP
                     VIIRLMRIDLSSSEIAVYATKEADRGLTTSDYAARVGAQVAINGDSFAVNGYVPRGLA
                     VGDSSTWANTADDARSAVFHVRRVGERTIAGISADSIVAPGDLPDGTEGVISGRPLLV
                     RQSQVATDQINCNDPITMDCQVAPRTAIAVSPDGNRLWLAVVDGWQNASYGMTLPDLA
                     EFLRQRGAGMAMALDSGSSSTLVQDGSPINVPSDGLERTVANHLAIKYGSLPKGELFG
                     LICKHSVVNCGTDVPSRKISGATVILDDTRTQTTDATGTFDFQSVTPRLACVTVKKTG
                     YLTVTQCRQVEPGQLTYNSVALWEGTDSPDAGVGEDAGIGTDASTVLDAQTSDAGNGY
                     DGPGGGCCDSGRDRPDVALLVVVAFILTRRRGTTA"
     gene            14949..>16014
                     /locus_tag="VFV99_07370"
     CDS             14949..>16014
                     /locus_tag="VFV99_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5059163.1"
                     /translation="MSTVHTQLLTDDASGLLVERAFRLRVVSGPDQGKEHMLDEGTTM
                     VGTHADNDLVLTDATVSRYHLEIRVRRDGIEVRDLDTTNGTKHGGARIGSVVLTGAAR
                     LRLGKHTEMDVEPVDKNVELGEWPSDRFGDVLGTTSPMKRLFALLSKAAPTEATILLQ
                     GETGTGKEAIAEAVHRHSRRSKGPFIVVDCGSIPHELIASELFGHAKGSFTGAGADKQ
                     GLIEAANHGTLFLDEIGELALDLQPQLLRVLDRRQVRRVGETQSVDVDIRVVAATHRD
                     LRAMVKSGHFREDLYYRLAVVATFVPPLRERKADIPALATWFAEKMGRGSFAQSPTLL
                     EQLQRHDWPGNVRELRNVVER"
BASE COUNT         2742 a         5343 c         5295 g         2634 t
ORIGIN      
        1 ccctgctcgc ctctgctctt cgcggcaaac gccgccgtca gtgccgcgtg tgcacgctcg
       61 ctctttgtcg cgaccatcac gccgctcgta tccttgtcga gccggtgcac gatgcccggg
      121 cgcagcacac cgccgatgcc cgacagatcc ttgcagtgga acaggagcgc gttgacgagc
      181 gtgccgctcg cgtgacctgc cgccggatgc acgacgaggc cggccggctt gttcacgacg
      241 atgaggtcgg gatcctcgaa caagatcgcg agcggaatcg gctcgggcaa aacctcgagt
      301 ggttccggcg ccggcacgcg cacgtcgata acgtcgccgt cgcggacgcg cgccgatttc
      361 gcggcggtca cgccgttgac ttgcacctga ccgtcttcga tcaggcgctg cacttgcgca
      421 cgcgacagcg attcgatggc cgccgcgatc acggcgtcga ggcgtccccc cgcctcgtcc
      481 gcaccaatga cgatctgcag ctcgttcgct gcgtcgtcgt ctaccaaatc ttcgatttga
      541 cgtgaccctt cgacttgacg agcacgtcga cgatcgcgcg ctcgtagatg tgctggccga
      601 agatgtcctc ggtgacgcgg ctcttgaaca gctcgcggcc ctcgttgatc tcgtcagaca
      661 tcacctcgaa gaggttgtcg ttggcgatgc cttcgagaat cttgggctcg tgatacaggg
      721 tcaggtccga agcgatggca cgcgcgagct ggcgcgcacg cttgggattg tcgatcaagg
      781 tcgccatgtc cggatcgtag gtgcgcgctt gcacccgggt caaggcttgg cggcttcgtc
      841 ctcggcctcg atcaccggcc cacgtgctgc acgataccgt tccagatacg tctgtttgac
      901 gcgttctgcg tcgagcccga gcgcgcgcgc gtattcggcg aggaagccac gcacgtacac
      961 cggcgccggg agcttcgcga acacttcgcc ttcgagcgcc tgcagatacg ccatcccgat
     1021 cttcgacttc tcggcaatct cgcgcagctc gacgcccacg gcttcgcgga tctgcctgag
     1081 cagcgggccc gagtactccg tgcgcgggct gatctccggc atcggcgggc gaatcgtcag
     1141 cgtcgctgga tcatcgacct tcgcggctgg ccgcggcgcc gagacgtcgg cgagtcgttc
     1201 gaccggcgtg atcggcatcg gcacgccatc ggggaacagc tcgaggtcgt actccttgcg
     1261 cttcgccgca tccatcagcg tcgtgtacgc gaggtcgagc cggcgatgaa ccgcttcgag
     1321 gctcaccgga tcgtagagcc cagagatcgc gatcgactcc gcaccataga tgtcgcgaat
     1381 gcggcgattg gcgcgccgga tgtcctcgaa gctcgcggtc ggcggcactt cgagcagctc
     1441 gtagtgcgag tcgctcggga ggacgtcggt atcgaacgtg tttgtcggga agccgccgga
     1501 gtcgctgcgg ccaaccggct tcaccgccag gagcccgcgc gcgacgcgct cgaagcactt
     1561 cgcgatgcgc gtctccgggt gctcgatgag cagcggacgc cgcctacgcg tcgaggccca
     1621 caccgcctcg tcgtactcga ggtgaccgag gtagcgcagc ggcgtgccga gccggcgacg
     1681 cgcggccgat gcgacggcac ggccgagctc catgtcgctc ttgctgcgcg ccgagttgat
     1741 cacgaggtgc ggtgcgaatc cgaggatcgc atcgcgcagc tcgccgagct cggagtgctg
     1801 tgccgcaacc gcgccgagat agacgtcgag cgcgctcggc gtgccgcctt cgaagtcgtt
     1861 cggttgcttg cccgcaatct gcgcgaggtg cgagaagcct ttgcgctcga gtcgcttcag
     1921 gaatgctgcg cgcacgaagc ggtgcatcag ctcgatcgat gtcggatcgg gcacgccgat
     1981 gagcagctgg atgtcagctt cgaggaacag ctctagcgtc gcggcgccca ggcccggccc
     2041 gagatcgacg acgacccagt cggcgggcag ctcgtgcagc tggtccgcga tcacacgcac
     2101 ctggtcggcg tcgggattcg cgatccaggg cggatcgtag acgccgccga tcagcttcac
     2161 gcccggcacg tgcgtcgaca ccgcgagctc ggtgagcggc gggccgcccg gcacgagcgc
     2221 ctcggcgaga ctgcgcgacg gccgcggcac accggcgaag atgtgaaggt tcggcgcacc
     2281 gaatgcgccg tcgagcagca cgaccttctt gccgagcgtg gcgaggaaga tgccgacgtt
     2341 ggcggctacc aggctcttgc ccacgccacc tttgccgccg ccgatagcga ccacccgagg
     2401 agtcatgctc ggcgggcaca taaccgcgtc tgtcgacggc ggtcaaggct tcagagagag
     2461 cgcaggtact gttcgaggtc gctttgctgt tcggcggtca gcgaaaggcc gagcacggtg
     2521 ttgtagtgac cgacgacgtc gagcagcgtg gctgcgctac cgtcgtggaa gtacggcgga
     2581 tgctgccaga caccacgaag cggcgtcgtg cggtacttgc cggtcgcgct ccgccgcgcc
     2641 tcgtccgtcg ccatgccggt ttcggtcgcc gcgtgcagcg tcggtgcgtc ggtgaacgac
     2701 gatccgctgt ggcagctggc acaccgtgcc tggccgtcga acagagtctt gccgcgtgcc
     2761 gcggcggccg cgtcgaagct gcccgcaggt ggcgccggcg gatcgaacga gaactgatat
     2821 tcgcgcagcg ctggcagcat cggcgtgacg cgatcgggct cctggtggat cgcgacgccg
     2881 attcgatcat cgacgaacga gccgtggccg cccatctgcg tgaccgcgac gtacgcgttc
     2941 cagtacgaga ccggaccctc gcccgtgtac gtctcgagcg cgacatcgcg caaaccgtac
     3001 gcgggcggaa tcaacaccgg atgactctcg gtgtcctggt tgaagcgcgc gtcgtacttg
     3061 ccggcgcccc agctctcgag cgcagccttg gcaaccgcgt tgtccacgcc gagcttggca
     3121 gtcagcgcat cgaggcccgg cgtcaacgaa acgatcgcgc cgggattcag atcgcgattc
     3181 ggccaaccat cgaggcgctt gccgatacct gccgtcaccg agttgtcgac ggtcgagtgg
     3241 cagagcgcgc acgtgatgcc gatcttcgtg atcgtgccgt tctcgacttc gccgcgcacg
     3301 ccgacgaccg cattcaggcc gatcagcgcg acggtcgtcg ctggattctc gagatcggcc
     3361 gacgcgagca cgtcaggtgg gacggcgtcg gcgtcgacct tcagaccaac cgaaagcgcg
     3421 gtcgtcggct tcaggttttg cacgagctcg tgcaatcgca gcgtgtcggt ccacagctgt
     3481 tcgtcaccga acgtgtcatg tcgaaaaatg cattgcccgt cggctacgtc acacggacct
     3541 tcggtggctg gcttgtcgcc gtctccgcac gcgacgagac acgcgaagac gatgacgttc
     3601 ttgatgcgca tgttctgctc cttcacgcat gagaggcaga cgcgcgaaga aacgtgacac
     3661 gtcacatcgg ctgtaacgcg caagcgcgat tgcgactcta ttcaggtagc aagcgaacga
     3721 agcgcttcga tcagctcgac gtagtcggtc tcggcgagcg tacgcacgtc gagccagagc
     3781 ttgccgtctt cgatgcgagc gatcactggc acgcgcgctg cgcgcagctt gcgatcgaga
     3841 gcctctgcgc tgcggccact gatcacgagc gcccacgagg gcagctgcgc cgtcggcatc
     3901 gcgccgccgc cgacggtcga catgcatccc tcgacggtga ccgtgaggcc ggctagccga
     3961 ccgagctctt cgcgcagccg ctgggcgtcg gcgtgcagcg cttccgacgt gcgtccgatc
     4021 atccgtacgg tcggtatttc gtcgaggttt gcgtcacggt agtgcgcgag cgtcgcggcg
     4081 agtgcggcaa tcgtgagctt gtcggggcgc agcgcgcgca tcagcgggtg cttgcgcgca
     4141 gtctcgaccg ccgccgtctt gccgacgagg atgccggcct gcggaccgcc gagtagcttg
     4201 tcgcccgaga acgtgacgag gtcggcgcca ctcgcgacgc attcggcgac cgtcggctcg
     4261 tcgggcagat gccagctgcg ctgcgtggcg cggtcgacga acatgcccga gccgagatcg
     4321 atcatcgtcg cgatgccctt ggcgcggccg atcgcgacga cctcttccgg cgagacctcc
     4381 gcggtgaagc cgacgatcgc gaagttcgaa cggtgcacct tgagtagcag gcccgtgttc
     4441 ggaccgatcg cgttctcgta gtcgcgcttg tgcgttttgt tcgtcgtgcc gatttcgacg
     4501 agcgtgccgc gcgagagcgc gaggatctcg gggagccgga acgagccacc gatctcgatc
     4561 agctcgccgc gcgaaacgac gatctcttta ttcgtcgcaa gcgccgcgag gccgagcacg
     4621 gtcgcggccg cgttgttgtt gcagacgatc gcgtcctcgg caccggtcag ctcgcggatg
     4681 agcgcgcgca ggtgatcgtg acgcgagccg cgctcgccct tgtcgaggtc gtactcgagg
     4741 ttcgaatagc cgcgggcgac ctcgtcgatc gcggcgcgcg ccgagtcggc aagtggagcg
     4801 cggccgaggt tcgtgtgaag cacgacgcct gtcgcgttga tcacgcggcg caatgctgga
     4861 cgcgcgagcg tggcggcgag ctcggcgact tcgagcgcgg cgatcgaaga gctgtcggcg
     4921 ttgccggcga ggatcgcggt gcggcgcgca gcgatcgcac ggcgcgcagc ttcgacgacg
     4981 gcccagcgcg gcaggctgcc gagcgcctcg gcttcgtccg cgagcgcgtg gacggccggc
     5041 aacttcgcca aacgtggatc cgacacgcgc gcaccctagc actatgcgat ggtaggtcgc
     5101 gatggccgac cccgacttgc cgcccgcagg cctgacctgg tgcgactacg tcgaacgtcg
     5161 ggtcgacgac tgcggtggtt ggctcccgct cgccgaccaa ttgattcatc gcgctggcga
     5221 cgcggtcgag atcgcgctcg atccgcagac ggttgagcgc ggcctgcgcc gactcgcgag
     5281 ccgcggccac aagtccggcg gccagtacgg ccgctggatg ctcaggttct tcggctcacg
     5341 tcgccgagcc accctcgcgc gcgtgctgcc agcgcagcca gccggtctcc tcgtcgacct
     5401 cgaacttgcc ggtcgtcccg gtcgggatct cgatcaccgc gttgaccgag ccatcgtcgt
     5461 tctgcgccgg atagccacgg ctgtaatggc gcggaccgat gacggtctca ctgtcgggca
     5521 cgtacgcgcg cggagagacc ggagcgcgcc cgacgaccgc ccgcatcggc acgtcggaac
     5581 aaggaagcca gacaacacaa caccaaacga atgcacgaaa tctcgacacg ttcagacagg
     5641 tacgcccgcc gcgcctggcc gaccaggaca tgatctcgga catctgcctg cgtgatcgat
     5701 cgcgcgcttg cgaaggatcc gcgcgagagg tttgcagatg ccgacgactg cgctgtcgac
     5761 ggccggcgtg tgaagcggcg gtggtggcaa tggttttgac gctgtagact gcgctggatg
     5821 gacgtccagg cagtctccgc gctgctcgcc gcgctgctga tcctcgcgat cggcacctcg
     5881 gtgctgctgc ggtcgcgctc ggacaagatg tacacgacgt tcgcggcgat gacgttcacc
     5941 gtgtcggcgt ggcatctgtg ctcgttcatc gcagccgcga cgggcagccc ggtgatgggt
     6001 tggctgtcgc tgtgggccgc cgccacgatc ccgccgaccg cgatccgctt cttccgcgtg
     6061 ttcctcgcgc agccatcgat cggcggcccg aagcgcggac cgcgcgtcac gctcgcgtgg
     6121 acgctgctcg cgtatgccgc gctcgtctac tccgcgatcg tccaaccgat tcacgagacg
     6181 acatggttcc tcgtgccgtt cggcatgtac gtattcggcg gcctctaccg ctgcgtctgg
     6241 gacatgttcg tgcagtaccg cgcgacgtcc aagcgcgtcg agcgcacgcg cgtcggctat
     6301 ctcacactcg gtggcttcgt cgcgacgact ctgacgctca ccgacgtact gccaaagctc
     6361 ggcgtcgcgt ggcccgcgat cggcaacgtc ctcagcatcc tctacctcta cttcctgtcg
     6421 cagacgctgt tccgctaccg gctgctcgac ctcaacgagc tcgtcggcaa gatggccgtg
     6481 ctcggcacgc tcgtcgcgct gctgtccgcc gtctacggct tcttgctgta ctgggtcaga
     6541 ggccagagca cccaggggct gctcctcctc aacgcgctcg tcgccgcgtt cgtgatcctc
     6601 atcctgttcg agccggtgcg taactggctc gagaacagca tcaaccgctg gctgttgcgc
     6661 cagcgcaccg agctgcgcgg ccggctcgaa gcagtgcgcc gcgagctgcc cggcgtcgtc
     6721 gacgtgcagg acatggtcgc gcgcatcatg acggcgctcg aggagtcacg gcgcgtcacc
     6781 gaggccggcg tgtatctgct cgacgccgac ggcgcgggct tcgattgcgc cggctcgctc
     6841 ggcaagacgc cgccggagcg gctcgacgcg aacgccgagc gcgcgctgct cgatcgcgtg
     6901 cgcgtcggct atctcgatct cgacgcgctg acccgcgagc tcgacgacct cggccaggct
     6961 ccggatgtcg aggcgaagcg caccgcgctc gtgtcgctca agacgcgcgt cgaggagctg
     7021 caggcgacgc tgatcttccc gctgttcggc tctgccgaaa ccgagcaagg cccgtggctc
     7081 ctcggcctgt tctgcgtgcg cgacgaccgc accgagagcg cgttcgatcc agacgacatc
     7141 gacacgttcc gtcagctcgc gatcggcgcc gcgcgcgtga tcgagtcatc gcaagcgtac
     7201 gagcgcgtga aagagcgcga ccgtctcgcc gcgctcggtg agatggccgc cggtctcgcg
     7261 cacgagatcc gcaacccgct cggcgcgatc aaaggcgcgg cacagctgct gatgacctcc
     7321 gacgggccgc agcagcatca caccgagaac gccgagttcc tccagatcat cgtcgaggaa
     7381 gcgaaccggc tgaacaacgt cgtgacgcgc ttcctcgatt acgcgcgcgc cgagcggccg
     7441 ggcaccgaag gcgccgacaa ggtcgacctc aacaacgtcg tacgcaagac ggtccagctg
     7501 ctccagaagg aacagctcaa gaacatcgag gtgcgcgtgc gcaccgacga gcagctgccg
     7561 caagtcgccg gcgatcccga atcgctgctg caagtcttct tgaacctcgg ccagaacgcg
     7621 atccaggcga tgcccgatgg cggcacgctc gagatcctga cgacacgccg gcgccgttca
     7681 cggctcggct acggccagtt ctgcgagatc cgcttccgtg acaccggcat cggcattccg
     7741 cgcgaccgcc tcaagaagct gttcatcccg ttctacacga cgaagcagaa gggcaccggt
     7801 ctcggcctcg cgatcagcca tcgcatcatc aaccaacacg gcggcaccat cgaggtgcgc
     7861 tcgacgatcg gccagggctc gacgttctcg gtgttcctgc cggcggccga gccgatgggg
     7921 aagatcgagg acatcaccga aaccggcaac ctcaagacgc tcccggacaa gccggagaag
     7981 ccggagaagc cggagaagtc agagaagccc gagacgccag ccgacgaacc cgtggtgccg
     8041 gcggccgcac ccgctacctc ttcgaccgga acgggataga gtaagcgccc gatgcagccg
     8101 gccaagatcc tcgtcgccga tgacgagcaa aacctgcgac gcgtgttgac cgcgctgctg
     8161 cgtcgcgaag gtcacgacgt gatccaggct gcgagcggcc tcgaagcgat cgaccgcctc
     8221 gctgacgtcg acgtcgtgat caccgacctg cgcatgccgg gcgccgacgg catggaggtg
     8281 ctgcgcaccg cttcgaagaa ccacccgcag gtcccggtca tcatgatcac ggcgtacggc
     8341 tcggtcggtc aggccgtcga agcgatcaag gccggcgcgt tcgactacat cgagaagccg
     8401 ttcgagcagg acgcgattcg catcatcatc gacaaggcga tcggccaggc cgctgcgaac
     8461 aagctcgcgc cccagcaaca gctctatctc tcgagcgagt cggagcagcg cggcaagttc
     8521 ggcctcgtcg gcacgtcagc ggagatgcag ggcatctttg ccgtgatcga gaaggtcgcc
     8581 gacacgccgt cgacggtgct catcaccggc gagtccggca ccggcaagga gctagtcgcc
     8641 aaggcgctgc acgaacagtc gagccgcaag ggcggtccgt tcatcaagat caactgcgcc
     8701 gcgattccca agacgctgat ggagtcggag ctgttcgggt acgaaaaggg cgcgttcacc
     8761 ggcgctacct cgagcaagcc gggtcgcttc gagctcgccg acggcggcac gctgttcctc
     8821 gacgagatcg gcgagattcc ggtcgagatg caggtcaagc tcctgcgcgc gatccaggaa
     8881 tcggagttcg agcgcgtcgg cggtctcaag acgatcaagg tcgacgtccg gttgatcacc
     8941 gcgacgaacc gcgacctcga gcacgaaatc gcacgcggca acttccggga ggatctgttc
     9001 taccgactca acgtcgtgcc gctccagatc ccgccgctgc gcaagcgcac gggcgacatc
     9061 ccgctgctcg tccagcacgt gatcaagaag ttcaacgaac ggctcaagaa gaacgtcacc
     9121 ggcatgtccg acgaggcgct cgccgctctc gagtcacaca actggccggg caacatccgc
     9181 gagctcgaga acgtgctcga acgcacgatc ctgttctgca agggcgaccg catcgagcgg
     9241 gccgatctcc agctcgtgct cgcgccgagc aaccaggcca gcaaccagtc gggctcgatg
     9301 gcgccgatcg aagcgccgct cgaagacgag atcgacgagc acgccgagat ctcgggctcg
     9361 ctcaaggaag tcgtccgggc cgagaccgcg cgcgtcgagc gcgagctgat cgtgaaggcg
     9421 ctcgacgaga ccggcggcaa cgtgacgcag gccgcacgcc tgctcaagat cagccgcaag
     9481 agcctgcaga tgaagatgaa ggaattcggc ctccgcgacc gcgagggctg atcgggcttc
     9541 gggcttcgga cttcggccag attagatcag gcggtaggca ggcccttcgt cttcacgttg
     9601 tgggcagcgg cccacttgcc gagctcttgc ttgagcggct tacccaggcc gcgcggcagg
     9661 ccgaatttct tgccgtcgaa ccggatgagg cgccaccaga tcgtctcctt gatctcttcg
     9721 atcttggagg cgtccatgat catcgagccc tcgttttgcg cgaccgcctg ctgcagctgg
     9781 ccttcgtcga cgacggtcgg cgcagctcct gcggtgggct gaaaccctgc agcgatcgca
     9841 ccgccgatcg caccgccgag accctggccg atcgccgcag cccatgcacc gcccgtcttg
     9901 gtgcagacga agtacagccg gtttggggcg aggaacacgt ggccgtggaa tcgcttcatg
     9961 ccgagctgaa gagtgagcgg gtatggagtc atggcgatca ttctagtcgt gatacgctgc
    10021 gtgcagggtg ctgcgaccgg tcagcgtgct tgtcgttctc gggctgtccg ggagcgcatt
    10081 cgccaatccg aattcgatcg tgcctggtgg tcaggatccc gactaccccg acgccggttt
    10141 cgcgcagaag ggcgtcgatg tctacggcga gatcgaatac gagtacgagc tcgatagctc
    10201 gaagatcgtg cgcgagcgcg tcggcgatcc cgcagcggat ccgctcgacg gcgtgacgac
    10261 cgtacgcgac ctcgacttca agcagtacaa gcacacgctg acgccgcggc tctacgtcgg
    10321 cgtgttccgc gacacgttcg tctacggcgc gctgccgatc gtgatcaccc aggcgcgcga
    10381 gctgtcgttc atcaatccgt cccgcgcatc gtcgacggtc aatgatggcc tgttgccgat
    10441 gcagggcttc gacgcgcgcg atccgggcac gcctacgccc ggcgacctcg cgttccgcgg
    10501 tcccgatcgt cacggactcg atcaggtgca cttcggcctc ggcaccgcgc tcatgaatca
    10561 gctcaaggac gacacgaagc cgacgtggaa gctgggcgcg gaagtgcggc tcgcgatcgg
    10621 cacgatcatg aagttcgatg cgatggcgcc cgaggcgaac aagggcgtgt cgaaaggcgt
    10681 gcaggagctg aagctgtgga cgacgttcgc acgcaagctc gggtgggccg agccgtgggt
    10741 cgagatgtac tggcaggtgc cgctctcgag caagagcggc tcgttgttcg acgatccggg
    10801 cttcggcgcg acgaacgtca agccgagcca gcaggcgggc atcgcgtcgg ggctcgagat
    10861 ctacgcgctc gacaaccgcg ccgaccagac gcggatcagc ttcgacatcg gcagcaaggt
    10921 cgatgcccac ttcgagggtc gcgagtacac cgagatgtgg gaggtgttcg cggcggccgg
    10981 cgacccgcgc tccatgggtc cgctcgtgct cgatgccgac ccgacgcgct cgggggtaca
    11041 ggcgctcagc caccccggca tctcgaacat cgagaactac ctcgagatga ccggtcggtt
    11101 cgccctgcgt gcgcagatcg gcccacacgt acgcttcggc gtgatcgctg acctgatctg
    11161 gaagacagat cacgtgattt cgttcgcaga tgcgggcgtc gaccgcgaag acgacaacga
    11221 cctagttaac ccaggcaccg acgaagttaa cccgctccat acgccggtca tcgacctcgt
    11281 cggtcaccgc tttcgcgcag aagacgcgtt cgacatcaac atcggcgtct ccggcaccgt
    11341 gctgttctag ccgcttctat aagagcttct cgggcaggca ctgacggtcg cgtctaggtc
    11401 gcggagtttc gaagtcgagc cggctgggct accattcgca ctagtgcttg cacgtcgaat
    11461 cattgctgta tcgccggaca agtccttcgg taagcagctc gcgacggcac tcaaggcggc
    11521 agggggtgcg gtcgaccttc atttgaagct cgacgacatg gccaagggcg agatcgtggc
    11581 ggccctggtc gtgctgcatc tcgatggcga gctcgcgacc gcggcggcgg agatcctgcc
    11641 tcgcctctcg tccgacgcga aggtgatcgc gatcattccg aagtcgaacc tcgcggcgat
    11701 cgtcgacatc atgcagtcgt ccgacaagat cgccggcatg ctgatggcgg agaacttcga
    11761 ctcccgccag ctctccgcga tggcgacgcg cgtgatcgcg ggcgacatct tcggcctcga
    11821 aaagaacatc tcgtggggca cgctggtcca caaccagctg gtgggcgact accaggagaa
    11881 gtcgctgtgc atctcgcaaa tctcggagtt cgccgagctg atgggcgtcc gccgcaagta
    11941 tcgcgaagcg atcgagcagt gcatcgacga gatgctcatg aacgcgctct acgacgcgcc
    12001 ggtcgacgag caaggcaagc cgctgttctc ggagattccg accaagacgc ggatctcgct
    12061 gcgcgtcgag cagaaggtcg tcgtgcagta cgcgtgcgac ggcaagcagt tcgcagtgag
    12121 cgtgcgcgat gcgttcggca cgctcgagcg caacacggtg ctgcggtacc tgtacaagtg
    12181 cctgcactcc gagcagcaga tcgatcgcaa ggtcggcggc gccggcctcg gtctgtatct
    12241 gatggtcaac tctgcgacga cggtcttctt caacgtgctg accggtgtcg cgaccgaggc
    12301 ggtgtgcgtc ttcgatctcg agacaccgaa gctgcagctc gagcgcttcg gcttcttcac
    12361 ggagaagatc gacgccggtg gccgactcgc cgctggtaca tcgcgccggt tgccgcaggg
    12421 ccatcccgtc gagcgccgtc gctcacctgc cccaccgccg ccagcgccgc gcggactcat
    12481 cgcagcactc tcggtcgcga tcctcgcggt gctcgtgctc gtcggcatcg ctgcatggcc
    12541 gcgcctgttc cccgccacgc agaccgcgaa ggtcacgttc acgacaattc ccaagggtgc
    12601 aacgatcgag atcgaagggc gcaacgaagg caccgccacc gagggtcagc tcgagcgcga
    12661 gctcgaagtc ggccgcgcgt atccggtggt cgcgcgcctc gacgggtacg agcccaagac
    12721 gagcgtcgtg cagccgcaaa ggggcggcag ccacgtcacg ttcgagctcg tggcgcgcac
    12781 cgcgaccgtg ttcctcgaca cgcaaccgac cggcgcgacg atcgaggtcg acggcaagtc
    12841 gctcggcacc acgccgctct cgatcacgac gctgccgccc ggcacgtcgg tgcagtacac
    12901 gatcaagaag accggctatc acccggcgac cggtacgctc gacgtgcccg ggccaggcaa
    12961 ggaagtccgc atggtgatgc cgctcgccgt gtcggacgag ctcgcgcgca tcaagctcac
    13021 gtcggatccc gccggcgcgc aggtctatca gaacggtcag ctgctcgcgg gcgtgcagac
    13081 gccggccgag gtgctcgtcg aagcaggcaa ggtccagcgc ttcatgctga ccatgccgaa
    13141 catggtgccg gcgttcatcg aaccgttcac gccggcccgt ggcgcggttg gcgtcgagaa
    13201 gaaggccagg ctcgtggccg gtcagccgct caggatcgaa gcgacgaacg acggcaaggt
    13261 cacggtcacc cctgcgcagc actgcaaaga gctgacgccg cccgccgagt gcgtcctcgc
    13321 gcccggcacg tacacggtcg actacaacgt cccgccgtcg aagttctcgc accaggtcac
    13381 ggtcggcacc aagcctgcca ccgacaagtt cgacctcggc ttcgtcgagg cagccgaggg
    13441 caagcacatc atgatcggcg gcaagcaggt caagaaggcg ctgttcgagg ccggctcgcg
    13501 ccaggtcacg attgctgacg acgccggcac gcgcaccgtg tccgtgaagg tcaaagccgg
    13561 cgccaccgtt accgccaagt agccgccaga ttaggcgaag tcatggcctg tgagcggcgg
    13621 ttacagagcg cgtgagggcg ctccagtatg ctgcgccgcg atgaaggtcg ccgtcgctgc
    13681 tctgctcgta ctcgccgccg aggcgagcgc agcgccgtcg ctgacgtcgt cgagtgatcc
    13741 gtatccaggc attcaccgcg agatctggcg cgacaccgat ccgcagcagc ccgtgatcat
    13801 ccggctgatg cgcatcgatc tctcgtcatc ggagatcgcg gtctatgcga cgaaggaagc
    13861 ggaccgtggg ctgacgacca gcgactacgc ggcgcgggtc ggtgcgcagg tcgcgatcaa
    13921 cggcgattcg ttcgccgtta acggttacgt gccgcgcggg ctcgccgtcg gtgactcgag
    13981 cacgtgggcg aacaccgccg atgacgcgcg cagcgcggtg tttcatgtgc gccgcgtcgg
    14041 cgagcgcacg atcgcgggga tttccgcgga cagcatcgtc gcgccgggcg atttgcccga
    14101 tggcaccgaa ggcgtgatct cggggcgacc gctgctcgtg cggcaatcgc aggtcgccac
    14161 cgatcagatc aactgcaacg acccgatcac gatggattgt caggtcgcgc cgcgcaccgc
    14221 gatcgcagtc tcgccggatg gcaacaggct gtggctcgcg gtcgtcgatg gctggcagaa
    14281 cgcgtcgtac ggcatgacgc tgcccgatct cgccgagttt ctgcggcaac gcggcgccgg
    14341 catggcgatg gcgctcgaca gcggcagctc gtcgacgctc gtgcaggacg gtagcccgat
    14401 caacgtgccg tccgatggcc tcgagcgcac ggtcgcgaat cacctcgcga tcaagtacgg
    14461 ctcgctgccc aagggcgagc tgttcggcct gatctgcaag cacagtgtgg tcaactgcgg
    14521 gaccgacgtg ccgagtcgga aaatctcggg ggcgaccgtc atcctcgatg acacgcgcac
    14581 gcagaccacc gacgcgacgg gcacgtttga cttccagagc gtcacgccgc ggctggcgtg
    14641 cgtcaccgtc aagaagaccg gctacctcac ggtgacgcag tgccgccagg tcgagcccgg
    14701 gcagctcacg tacaactcgg tcgcgctgtg ggaaggcacg gattcgcctg acgcgggtgt
    14761 cggcgaagat gcaggcattg gcacggatgc gtcgacggtg ctcgacgcac aaaccagcga
    14821 cgctggcaac ggttacgacg ggccaggtgg tggctgctgc gacagtggtc gcgatcgtcc
    14881 cgatgtcgcg ctcttggtcg tcgtcgcttt catcctgacg cgccgtcggg gtacaaccgc
    14941 ctagggacgt gagtacagtc catactcagc tacttacgga tgacgcctcc ggcctgctgg
    15001 tcgagcgcgc gttccggctc cgcgtggtct ccggccccga tcagggcaaa gagcacatgc
    15061 tcgatgaggg cacgacgatg gtcggtacgc acgccgacaa cgatctcgtg ctgaccgacg
    15121 cgaccgtgtc gcgctaccac ctcgaaattc gcgtgcgccg cgacggcatc gaggttcgcg
    15181 acctcgatac gacgaacggc accaagcacg gcggtgcgcg catcggcagc gtcgtgctga
    15241 ccggcgctgc acgcctgcgt ctcggcaagc acaccgagat ggacgtcgag ccggtcgaca
    15301 agaacgtcga gctcggcgag tggccaagtg atcggttcgg cgacgtgctc ggcacgacat
    15361 cgccgatgaa gcggctgttc gcgctgctct cgaaggcggc gccgaccgaa gcgacgatcc
    15421 tgctccaggg cgaaaccggt accggcaagg aagcgatcgc cgaggccgtg catcgccaca
    15481 gccgccgcag caagggcccg ttcatcgtcg tcgactgcgg ctcgatcccg cacgagctga
    15541 tcgcgtccga gctgttcggt catgcgaagg gtagcttcac cggcgccggc gccgacaagc
    15601 agggactgat cgaagccgcg aatcacggca cgctgttcct cgacgagatt ggcgagctcg
    15661 cgctcgacct ccaaccgcag ctgctgcgcg tgctcgatcg ccgccaggtg cgccgggttg
    15721 gcgagacgca gtccgtcgat gtcgacatcc gcgtcgtcgc cgcgactcac cgcgacctgc
    15781 gcgcgatggt gaagtccggc catttccgcg aggacctcta ctaccgtctc gcagttgtcg
    15841 cgacgttcgt gccgccgctg cgcgagcgca aagcggacat ccctgccctc gcgacctggt
    15901 tcgccgagaa gatggggcgc ggcagcttcg cgcaatcgcc gacgctactc gagcaactac
    15961 agcgccacga ctggcccggc aacgtgcgcg agctgcgtaa cgtcgtcgag cgcg
//
LOCUS       DASUFX010000185         9074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_583908,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000185 DASUFX010000000
VERSION     DASUFX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583908"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV99_07375"
     CDS             complement(<1..336)
                     /locus_tag="VFV99_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059164.1"
                     /translation="MSVIIYGERRYGTVEAYGGEYAQTRFFHIWFVPLIPVGSHWMTS
                     RNAGYTIGLHGKSVLAAYGRVWGPAIALGTFVAGVGGNPLMLLFSALAIAATVVAWSW
                     RNLRGEAAIR"
     gene            complement(402..2594)
                     /locus_tag="VFV99_07380"
     CDS             complement(402..2594)
                     /locus_tag="VFV99_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059165.1"
                     /translation="MRLALAIIATLGLCACGSHETTVTTPDVVVRHEARLPSTPGKHA
                     PSLVAIENETPGELSTGTMLADVDSCATCHPDVAAQWSSSAHSFASFGNPIYRFDVEL
                     VRHDLGKTPSRHCGGCHDMPLMVDGLMTGDAPIPASDLRAHSGVTCRLCHGVQQTTHD
                     GNGSYVWNAAPIDAPVLGDAASIAKHKKQVTTKVDTELCVGCHRGFLSPDMTMPVHLS
                     GIDEPGNWRNSAYTGNGMARIDNVEKQNCIDCHMQRTQVSSAELGAKQGSVASHRFVG
                     GHTWMAAMRGDTEQLKLTRAKLEGAASIDVAGARVVKDGKPGDWHLPADGAPVEPGTR
                     LELDVVIRNLLVGHRFPGGVLDIQDTWVELELVDAKGKRIAASGLRHETDPNDEDTHV
                     LRTLVVDKNGEVLERHEMGQFRTQVATQTLAPREAQAVRYAFDVPARIALPVAVNARL
                     RHRARTLKMQAATCEAARTEEGKAFIAGARGARYVDFDPCKPQPVTLVAQTRIEIGAG
                     AKISTARPAWERIYEHGMALGAMVVLRVPEAAAVLDVAYKLAPDARAKAMVAVQLGWV
                     ASKLGKLDTALARVKEARELLATTVAPGQPAPNPPVFDALTADVYMRLNKYKEAVAPA
                     KSCTERAPSNASAWSVYARALVAVGDYTKALETARKGLELSPRDPDLLRSQATSLAAL
                     KDPQMEAAQNAYVRFRVPDEAAVLRIRCAKGEARCYRNRNPVQTIVLR"
     gene            complement(2594..4537)
                     /locus_tag="VFV99_07385"
     CDS             complement(2594..4537)
                     /locus_tag="VFV99_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX5059166.1"
                     /translation="MREVRHALAAGLALLTAGAVLLAQTPVAQACGCLSPPAVTEGQY
                     AVNQSAEQIIFETEPGWVTAHVLIKYAGDPAKFAWIIPVPEVPELAISPVSAFGLLDK
                     LTAPDISVSSENICPISEWTCQYNDPVYCGGFGGADDLGEGGGSGAGASADAGAGAPG
                     DPVTVINEQVVGDYQTVTFRASEAAAATQWLRDNGFIVNQTTSIYMESYVQANMVFVA
                     AKLIPGAGVKAIKPLKMRYRAAYPMVPLILTAVAAEPHLTVTTFIYGDRPFRPLGHPV
                     VEINPERIARDSANRSNYPQVLARTIDEAGGDAFAIEYRGSPLASNLGNSFCCQNSYD
                     SCNVGNNGQCECPGSTLDEADCSKQGDLNDGVKLLDELATKYTALTRITTRVSPEEMT
                     FDPQYEPDFAAQFTGRLTARGTQPSLSGCTTQVIDQKQLAKIDALQRCAATYCGAGGR
                     CGTTQFSSGCECAPGFVSQRFTDLDGQPSVTCIPETPPVDLRAGGFQLPDACAGVNCG
                     MGSCVDRNGVATCKCNEGTVAVAGNGTVPHCDPIQFATYTQGAQDYSEALRGLAVCAP
                     AYPSCGEDGWLVKQKSPRPGVACGNVNDPPPWLTTPKPQKTCGAFGCGGCETSGGGPL
                     SIGIAWVVLLLVLRPRRRRAKAD"
     gene            complement(4673..5167)
                     /locus_tag="VFV99_07390"
     CDS             complement(4673..5167)
                     /locus_tag="VFV99_07390"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX5059167.1"
                     /translation="MALIRLVPMLAFVIACGGKSPAPSAPAPIAPAAASKLLVLVDDQ
                     GVGLGGNDPIAYAIDKAAVGGNTEHSSQHGGATYRFASAEHKATFDGAPAKHSPQYGG
                     YCAFAASQNRLSEADPAVFQIVDGQLLVFTNADFKDQFNKDVAGNKQKADQNWPGLVE
                     RYGK"
     gene            complement(5185..5418)
                     /locus_tag="VFV99_07395"
     CDS             complement(5185..5418)
                     /locus_tag="VFV99_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059168.1"
                     /translation="MSFAYTDQTIRVRKLPTGPTRNLRQQFAVERPRRWPYVVIVALA
                     AFALATPVRMPERAPTGAERTTEAQQLLRLLGR"
     gene            5614..6249
                     /locus_tag="VFV99_07400"
     CDS             5614..6249
                     /locus_tag="VFV99_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5059169.1"
                     /translation="MRILIDVANGRVSEELLDVLGRAGHEVERGSVATKRRFDVVVVG
                     SVDVAERLRRERPTDAIIVFTRVGDVEARIRALDIGASDAVDASFPPSQIAARFGAAG
                     RRAAAIPRDPDRIAIDGCTIDLSACTGERDGSAFALTKREVDIVRWLSRHAGQVVSRG
                     DLLQHVWGLSPRTETRSVDVAIAGLRAKLERNPSEPAIIISVKGAGYRWGA"
     gene            complement(6266..6472)
                     /locus_tag="VFV99_07405"
     CDS             complement(6266..6472)
                     /locus_tag="VFV99_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059170.1"
                     /translation="MNDLTVGIRVFSATKAKDRAGLGDVVTEWIRAHPQFEIFDRRVL
                     QSSDSDFHCLTIVLLFHYVVPLVN"
     gene            complement(6538..6936)
                     /locus_tag="VFV99_07410"
     CDS             complement(6538..6936)
                     /locus_tag="VFV99_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059171.1"
                     /translation="MITSETMGAAASRAWLEERRLDAKKIRHWFVEIVIDSDSPDAVL
                     DLNIYPEEWGFVFRRGNRVSSIRITDIPFVHGRDDFGLLPETPSLDRIGDLLATLERR
                     FDFRFQRVGPRVKTNLVRGAMVVGAWLVHQ"
     gene            7082..>9074
                     /locus_tag="VFV99_07415"
     CDS             7082..>9074
                     /locus_tag="VFV99_07415"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059172.1"
                     /translation="MAGRPLILVSATLVAALSVLVLIGLRVLERDRAELLARYGQSRE
                     QVIEEAARGVAAEVADIGEDLDLASTLLQSAETTQLAERELHAIATIKREYLVMYARA
                     NGSDATTKVTAFDAPPGVAAKADATLERLLKLAEDQPGELHVSTAFADSHDSAAWYRV
                     YARKVKAGRPVVAVVVDTAVLLSRMKLQRDAMARVAVLDATGTPAALSDPALATVVRI
                     GTPTRTVIDHEIAHRIGLPKSDAVVAAVPLVVDGGPAWTLLMAASTQSLQTQQRIIVR
                     RVLLGSSLVLVLLLSAAAYVLHNTFRARALRERLKHADRLAHLTEKAEKILDHIPSGV
                     LALSENLTVTGVNRWLAERIGRDVIGRTLDSTFEAGRSNDIELVVSLVKRALDTREPQ
                     ALHGERISLLGQEASLNMHAVPLARGIGDVSVLLVLEDLTELRRIEQRLLHSEKLVTA
                     GQLAAGIAHEIGTPLNVARGRVELTLSHLGSQHAESENHRLVIDQIDRVTRLIQQLLD
                     YVRPAPAAIQEIDLAQSLHGVRDLLAARAAKRSVLLDVDVSPDASTLRTDPDQMQQII
                     VNLALNAIDACDKGGNVTLRSKRRDDSVVLEIEDTGHGIPRDLQKQVFDPFFTTKKRG
                     QGTGLGLWVVAQLVRAQSAEIELDSVPGRGTTVRVAWPVL"
BASE COUNT         1539 a         3037 c         2979 g         1519 t
ORIGIN      
        1 gcgaatcgca gcttcaccgc gcaggttgcg ccacgaccat gccaccacag tcgccgcgat
       61 cgcgagcgcc gagaacagca gcatgagcgg attgccgccg acgccggcga cgaatgtgcc
      121 gagcgcgatc gccggtcccc acacgcgccc gtaggccgcg agcacgctct tgccgtgcag
      181 accgatcgtg tagccggcgt tgcgcgacgt catccagtgc gagccgaccg ggatcagcgg
      241 cacgaaccag atgtggaaga accgggtctg ggcgtattcg ccgccgtacg cttcgacggt
      301 tccgtaacgc cgctcgccat agatgatcac ggacacgcgc ggggcgtatc acatcgcgcc
      361 cgtcgacgac gaaacatttt gcgacagtcg cgtcggcgca attagcgcaa cacgatggtt
      421 tgcacggggt tgcgattgcg atagcagcgc gcctcgccct tcgcgcagcg gatccggagc
      481 acggccgctt cgtcgggaac gcggaatcga acgtacgcgt tctgcgccgc ctccatctgc
      541 ggatctttga gcgcggcaag cgacgtcgcc tgcgagcgca agaggtccgg atcgcgaggc
      601 gagagctcga ggcccttgcg ggcggtttcg agcgctttcg tgtagtcgcc gaccgcgacc
      661 aacgcacgcg cgtacacgct ccacgcggag gcgttactcg gggcgcgctc ggtacacgac
      721 ttcgccggcg cgactgcttc cttatacttg ttgagccgca tgtagacatc ggcggtgagc
      781 gcgtcgaata ccggcggatt cggtgccggc tggcccggag caacggtcgt cgcgagtagc
      841 tcgcgcgctt ccttcacgcg ggcgagtgcg gtgtcgagct tgccgagctt ggatgcgacc
      901 cagccgagct gcactgcgac catcgccttg gcgcgcgcat cgggcgcgag cttgtacgcg
      961 acgtcgagca cggcggccgc ttcgggtacg cgcagcacga ccatcgcgcc gagcgccatg
     1021 ccgtgctcgt agatccgctc ccaggcgggc cgcgccgtcg agatcttcgc gcccgcaccg
     1081 atctcgatgc gcgtctgcgc gacgagcgtg accggttgcg gtttgcacgg atcgaagtcg
     1141 acgtagcgcg caccgcgggc acccgcgatg aacgccttgc cttcttcggt gcgcgccgcc
     1201 tcgcaggttg ccgcctgcat cttcagcgtg cgcgcgcgat ggcgcagccg cgcgttaacc
     1261 gcaacgggca gcgcgatccg tgccggcacg tcgaacgcgt agcgcaccgc ttgggcttcg
     1321 cgcggcgcga gcgtttgcgt cgcgacctgc gtgcggaact ggcccatctc gtggcgctcg
     1381 agcacctcgc cgttcttgtc gacgacgagc gtgcgcagca cgtgcgtgtc ttcgtcgttc
     1441 ggatcggtct cgtgccgaag gcccgacgcc gcgatgcgct tgcccttggc atcgacgagc
     1501 tcgagctcga cccacgtgtc ctggatatcg agcacgccgc cagggaagcg gtggccgacg
     1561 agcaagttgc gaatcacgac gtcgagctcg aggcgcgtgc ccggctcgac gggggcaccg
     1621 tcggcgggca agtgccagtc gccaggcttg ccgtccttca cgacgcgcgc accggcgaca
     1681 tcgatcgacg ccgctccttc gagcttcgcg cgcgtgagct tcagctgttc ggtgtcaccg
     1741 cgcatcgcgg ccatccaggt gtgaccaccg acgaagcgat gcgacgcaac actgccctgc
     1801 ttcgcgccga gctcggccga cgacacctgc gtgcgctgca tgtggcaatc gatgcagttc
     1861 tgcttctcga cgttgtcgat gcgcgccatg ccgtttccgg tgtacgcgga gttgcgccag
     1921 ttgcccggct cgtcgatgcc ggacaggtgc acgggcatcg tcatgtcagg cgagaggaac
     1981 ccgcgatggc aaccgacaca cagctcggta tccaccttcg tcgtcacctg cttcttgtgc
     2041 tttgcgatcg acgccgcgtc gccgagcacc ggcgcatcga tcggcgccgc gttccacacg
     2101 tagctgccgt tgccatcatg cgtcgtctgc tgcacgccat ggcacaagcg gcacgtcacg
     2161 ccggagtggg cacgcagatc gctcgccgga atcggcgcgt cgccggtcat tagcccgtcg
     2221 accatcagcg gcatgtcgtg acaaccgccg cagtggcggc tcggcgtctt gccgagatca
     2281 tggcgcacga gctcgacgtc gaagcgatag atcggattgc cgaacgacgc gaacgagtgc
     2341 gcgctcgacg accactgcgc ggcgacgtcg ggatgacacg tcgcgcagct gtcgacgtcg
     2401 gcgagcatcg tgccggtgct gagctcaccc ggcgtctcgt tctcgatggc gacgagactc
     2461 ggcgcgtgct tgcccggtgt gctcggcagt cgcgcttcgt ggcgcacgac gacatcgggt
     2521 gtcgtgacgg tcgtctcgtg actaccgcac gcacacagcc cgagcgtcgc gatgattgcg
     2581 agcgctagcc gcatcagtcc gcctttgctc tgcggcggcg cggccgcagg acgagcagaa
     2641 gaacgaccca cgcgatgccg atgctgagcg ggccaccacc cgaggtttcg cagccgccgc
     2701 aaccgaacgc accgcacgtc ttctgcggct tcggggtcgt gagccacggc ggcggatcat
     2761 tcacgttgcc acacgcgacg ccgggacgcg ggctcttctg cttgacgagc cagccgtcct
     2821 cgccgcacga cggatacgcc ggcgcgcaca ccgcgaggcc gcgcagcgct tcgctgtagt
     2881 cctgcgcgcc ttgcgtgtag gtcgcgaact ggatcggatc gcagtgcggc accgtgccgt
     2941 tgccggcgac cgcgaccgtg ccctcgttgc acttgcacgt ggcgacgccg ttgcgatcga
     3001 cacacgagcc catgccgcag ttcacgccag cgcaggcatc gggcagctgg aagccaccag
     3061 cgcgcagatc gaccggcggc gtttcaggga tgcaggtcac cgacggctgg ccgtcgaggt
     3121 cggtgaagcg ttgcgacacg aatccgggtg cgcactcgca gcccgagctg aattgcgtcg
     3181 tgccgcagcg accaccggca ccgcaatacg tcgcggcgca gcgctgcagc gcatcgatct
     3241 tcgcaagctg cttctgatcg atcacctgcg tcgtgcaacc agacaggctc ggctgcgtac
     3301 cgcgcgcggt gagccggccg gtgaactgcg ccgcgaagtc cggctcgtac tgcggatcga
     3361 acgtcatctc ttcgggcgag acgcgcgtcg tgatgcgagt cagcgccgtg tacttggtcg
     3421 cgagctcgtc gagcagcttg acgccgtcgt tgaggtcacc ttgtttcgag cagtctgcct
     3481 cgtcgagcgt cgagcccgga cactcgcact ggccgttgtt gccgacgttg cacgagtcgt
     3541 acgagttctg gcagcagaag ctgttcccga ggttcgacgc cagcggagag ccgcgatact
     3601 cgatcgcaaa cgcgtcaccc cccgcctcgt cgatcgtgcg cgcgagcacc tgcggatagt
     3661 tcgaacggtt cgccgagtcg cgcgcgatgc gctcgggatt gatctcgacg accggatggc
     3721 cgagcggacg gaacggacga tcgccgtaga tgaacgtcgt caccgtcagg tgcggctcgg
     3781 cagcaaccgc cgtcaagatc agcggcacca tcgggtacgc cgcgcggtag cgcatcttga
     3841 gcggcttgat cgctttgacg ccagcgcctg ggatcagctt cgccgcgacg aacaccatgt
     3901 tcgcctggac gtacgactcc atgtagatcg acgtcgtctg attgacgatg aagccgttgt
     3961 cgcgcagcca ctgcgtcgcg gccgccgctt cgctcgcgcg gaacgtgacc gtctgatagt
     4021 cgccgacgac ttgctcgttg atgacagtga ccgggtcgcc gggcgctcct gcgccggcat
     4081 ccgccgatgc accagcaccc gaaccgcccc cctcgccaag atcgtccgcg ccgccgaagc
     4141 caccgcagta gaccggatca ttgtactggc aggtccactc cgagatcggg cagatgttct
     4201 cgctcgagac cgagatgtcc ggcgccgtca gcttgtcgag cagaccgaac gccgacaccg
     4261 gcgagatggc gagctcgggc acctcgggga ccggaatgat ccacgcgaac ttcgccgggt
     4321 cgccggcgta cttgatcagc acgtgcgcgg tgacccagcc gggctcggtc tcgaagatga
     4381 tctgctccgc gctctgattg accgcgtact ggccttcggt aacggccggc ggcgacaggc
     4441 acccgcatgc ctgggcaaca ggagtctgag ctagaaggac agcgcctgcg gtgagcagcg
     4501 ccagacctgc ggctagggcg tgacggacct cgcgcatcct atccgctcta ccaagcgccg
     4561 agccaaggtc agctgagtag atccagcgcg ttagcggcgg cgctttcaaa aacccgcgac
     4621 cgaaccacga cagcggtgtc agagatccga ggcgcgagca gccgcgctat cgctacttac
     4681 cgtagcgctc gacgagtcct ggccagttct ggtcggcctt ctgcttgttg ccagcgacgt
     4741 ctttgttgaa ctggtctttg aagtcggcgt tcgtgaacac gagcagctgg ccgtcgacga
     4801 tctggaacac cgcggggtca gcctccgaga gtcgattctg cgacgcggcg aacgcgcagt
     4861 agccgccgta ctgcggcgag tgcttggccg gtgcgccgtc gaacgtcgcc ttgtgctccg
     4921 ccgaggcgaa gcgatacgtc gcgccgccgt gctgcgagct gtgctcggtg ttgccgccaa
     4981 cagctgcctt gtcgatggcg tacgcgatcg gatcgttacc gccaagtccg acgccctggt
     5041 catcgacgag caccagcagt ttgctcgcgg ctgctggagc gatcggtgct ggggcactcg
     5101 gtgccggcga tttgccacca cacgcaatca cgaacgcgag catcgggacg agtcggatca
     5161 acgccatgcc cgcatcttag cgagctagcg gccgagcagg cgaagcagct gttgcgcttc
     5221 ggtcgtccgc tcggcaccgg tcggcgcgcg ctccggcatg cgcaccggcg tcgcgagtgc
     5281 aaacgcggcg agtgcgacga tcacgacgta cggccaccgc cgcggcctct cgaccgcgaa
     5341 ctgctggcgc aagttcctcg tcggcccggt cggcagcttc cgcacccgga tcgtctggtc
     5401 ggtataggcg aacgacatga gcgttctcct tgcgacggcg atgccagcgg cgagatccgc
     5461 gacgagaccg cgatccgtgc acgcgatgtg cagccgcctg cacgtgccgc cagcgccgga
     5521 tcacgatgcg tgcacgagca acaagaatcg ccatgggagc tgcgtcccgc actgtgcaca
     5581 cgacctgcac cagtacatca gtacaatgag gcagtgcgca tcctgatcga cgtcgcgaac
     5641 ggccgtgtca gcgaagagct gctcgacgtg ctcgggcgcg cgggacacga ggtcgagcgc
     5701 gggagtgtgg caacgaaacg ccggttcgat gtcgtcgtcg tcggctcggt cgatgtcgcg
     5761 gagcgattgc gccgcgagcg gccgaccgac gcgatcatcg tgttcacgcg cgtcggcgat
     5821 gtcgaagcgc gaattcgtgc gctcgatatc ggtgcgagcg acgcggtcga cgcgagcttt
     5881 ccgccgtcgc agatcgcagc gcgcttcgga gctgcaggcc gtcgtgccgc cgcgatcccg
     5941 cgcgatcccg atcgcatcgc gatcgatggc tgcacgatcg atctatcggc gtgcaccggc
     6001 gaacgtgacg gcagcgcgtt tgcgctgacg aagcgcgagg tcgacatcgt gcgctggctg
     6061 tcgcgtcacg ccggccaggt cgtcagtcgc ggcgatttgc tccagcacgt ctggggactg
     6121 tcgccgcgca ccgagacacg ttccgtcgac gtcgcgatcg cgggcctgcg cgcgaagctc
     6181 gagcgcaacc cgagcgagcc ggcgatcatc atctccgtca aaggcgccgg ctatcgctgg
     6241 ggcgcatgac cgccgcctca cacgatcagt tcacgagcgg caccacatag tgaaacagca
     6301 gcacgatcgt caaacagtga aaatcggagt cggacgattg caacaccctc ctgtcgaaga
     6361 tctcgaactg gggatgagcg cggatccact cggtgaccac gtcgccgaga ccggcgcggt
     6421 ctttcgcctt cgtcgctgag aacacccgga ttccaacagt gagatcattc atgtgctagc
     6481 tcccgacgac ggcaacaacg accacgacga ctgcatgcgc actcccgcgg tcgcgtttca
     6541 ctggtgcacc agccacgccc cgacgaccat cgccccgcgc accagatttg ttttgactcg
     6601 cgggccgacg cgctgaaacc ggaagtcgaa gcgccgttcg agcgtagcga gcaaatcgcc
     6661 gatccgatcg agcgagggtg tctcggggag cagcccgaaa tcgtcgcgcc cgtgcacgaa
     6721 cgggatgtcg gtgattcgga tcgatgacac gcggttgccg cggcgaaata cgaatcccca
     6781 ctcctcgggg taaatgttga ggtccaggac agcgtcgggc gaatcggagt cgatcacgat
     6841 ctcgacgaac caatgacgga tcttcttggc gtcaagcctg cgctcttcga gccacgccct
     6901 gctggccgcc gcacccatgg tctcgctcgt gatcacgctt gcgagccgcg tgcgagcatc
     6961 tcctgtgccg aggccctggc gacgggcagg gctctacgtg cggcaaagtg caacgctggt
     7021 gtggcgttct gcaagaccgc cttctccgcc cgattcgagc ggatggtggt acaccgaacg
     7081 cgtggctggg cgccctctga tcctcgtcag cgccacgctc gtcgcagcgc tgagcgtgct
     7141 ggtgctgatc gggctgcgcg tgctggaacg cgatcgcgcc gagctcctcg cacgctacgg
     7201 gcagtcgcgc gaacaggtga tcgaggaggc cgcacgcggc gttgccgctg aagttgctga
     7261 catcggggag gacctcgacc tcgcctcgac gctgctgcag agtgcagaaa ccacacagct
     7321 cgccgaacgc gagctccacg caatcgcgac gatcaagcgc gagtacctcg tgatgtacgc
     7381 acgtgccaat ggcagcgacg ccacgaccaa ggtcacagca ttcgatgcac cgcctggggt
     7441 cgcagccaag gccgacgcga cgctcgagcg cctgctgaaa ctcgccgaag accagccagg
     7501 cgagctccat gtgtcgactg cctttgctga ttcacacgac tcggctgcct ggtaccgcgt
     7561 ctacgcgcgc aaggtgaagg cgggtcgccc cgtcgtcgcc gtcgtggtcg acactgccgt
     7621 gctgctgtcg cgcatgaaat tgcagcgcga tgcgatggct cgcgtagcgg tgctcgacgc
     7681 gacgggaacc cccgcagcgc tgagcgatcc cgcgctcgcg acggttgtgc ggatcggaac
     7741 cccaacgcgt accgtgatcg accacgagat cgctcatcgc atcgggctac cgaagagcga
     7801 tgcggtcgtt gccgcagtgc cactcgtcgt cgatggtggg ccagcgtgga cactgctgat
     7861 ggcggcatcg acgcaaagcc tgcaaaccca gcagcgcatc atcgttcgcc gcgtcctgct
     7921 cggcagcagc ctcgtgctcg tgctgttgtt gtcggcagcc gcctacgtcc tgcacaacac
     7981 gtttcgcgca cgcgcgctcc gcgagcgctt gaagcatgcg gatcggctgg cgcatctgac
     8041 cgagaaagcc gagaagatcc tcgatcacat tcccagcggc gtgctcgcat tgtcggaaaa
     8101 cctgacggtc acgggcgtga atcgctggct tgcggagcgg atcggtcgcg acgtcatcgg
     8161 ccgcacgctc gactcgacgt tcgaggcagg acgaagcaac gacatcgagc tcgtcgtcag
     8221 cttggtcaag cgcgcgctcg atacccgcga gccccaggca ctgcatggcg agcggatctc
     8281 cctcctcggt caggaagcgt cgctcaacat gcacgcggtg ccgcttgccc gcggcattgg
     8341 cgatgtcagc gtcttgctcg tactcgagga tctgaccgag ctgcggcgaa ttgaacagcg
     8401 cctgctgcac tcggagaagc tggtcacagc aggtcagctc gccgccggaa tcgcacacga
     8461 gatcgggacg ccgctcaacg tcgcccgcgg tcgcgtcgag ctcacgctct cgcatctcgg
     8521 cagccagcat gccgagtccg agaatcatcg gctcgtcatc gatcaaatcg atcgagtgac
     8581 gcgcctgatc caacagctgc tcgattacgt gcggcctgcg cctgcagcca tccaggagat
     8641 cgacttggcg cagtcgctgc atggcgtgcg ggacctgctc gcagctcgag cggcaaagcg
     8701 cagcgtattg ctcgatgtcg atgtttcgcc ggatgcgtcg acactccgaa cggatccgga
     8761 ccagatgcaa cagatcatcg tgaacctcgc gctcaatgca atcgacgcgt gcgataaggg
     8821 cggcaacgtc acccttcgca gcaagcgccg cgacgatagc gtcgtgctgg agatcgagga
     8881 taccgggcac gggattccgc gtgacctcca gaagcaggtg ttcgacccat tcttcacgac
     8941 gaagaagcgc ggacagggca ccgggctcgg cctctgggtc gtcgcccagc tcgtgcgcgc
     9001 gcagtcggca gaaatcgagc tcgatagcgt tccggggcgt ggaacgaccg tgcgcgtcgc
     9061 atggcccgtg ttgt
//
LOCUS       DASUFX010000186         9529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_445596,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000186 DASUFX010000000
VERSION     DASUFX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445596"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1434)
                     /locus_tag="VFV99_07420"
     CDS             complement(139..1434)
                     /locus_tag="VFV99_07420"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5059173.1"
                     /translation="MSRKGDHIKRVGLVFSGGPAPAANAVISSAAISFLEDSREVIGF
                     FHGYSNLEEYHPVSHRLLPDEHYRIFELKDLSGLRNTRGIVIGTARSNPGKGIEKAAD
                     LDDLNKTKKLRNVYNAMVDLEIDALVSIGGDDTLKTANFLYEYQKRLPPDARRVQVIH
                     LPKTIDNDYRGIDFTFGFFTAVDVMAKELQNLRADAIATSSYFIVETMGRKAGWLSYG
                     VAIAGEANLVLAVEDITGPLTVEGKNKHLSLDALVDRIVDLVIAREKRGKYYGTVVLA
                     EGLAEMLPETQIANLPLDEHGHLPLGRFDLGKLVAQLVSKRHEQRTGRKKKLTGLQLG
                     YESRCAPPHAFDVMLGSQLGIGAYRALVEENLDGHMVSVSGQLDLNYVPFKDLINPTN
                     LKTEVRFIKTGSDYHKLARFLETRTDKIVEWAPGRREER"
     gene            complement(1454..2632)
                     /locus_tag="VFV99_07425"
     CDS             complement(1454..2632)
                     /locus_tag="VFV99_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059174.1"
                     /translation="MREGYTLWQIAAMRFMQVWVVVAAVVASGANARADKPRELLDEA
                     KALLVVGACADGTPPATVTAKTVEAHCKTIRKAQDAYKTGWVTPVREFFAAHVPATVP
                     KIVVYPFAGGDLSTALTVYPDASEITTLSLEPAGDPRALSRMDDKQVRVALKTVEKEL
                     SSLYRSSYSKTMDMIGAMRGAQLPTQLIFSLSALTIHGYEPVAMRYFRLTTDGDIEYL
                     SDADFAAIDKIEDVGKRNQQLSNVEIKFKKQGGTREQIYRHIMANLDDKHLKAEPNAL
                     VHLEKKGNVAAMTKAASYLLSFGEFQAMRKYIIDHVDWMVSDSTGLPPKYGTPAGFEY
                     ETYGSYEASNMKAGGEVTPQWVALYKQQPKRPLTFRFGYPDKKWRGHLILMGRAPKQK
                     "
     gene            2631..3395
                     /locus_tag="VFV99_07430"
     CDS             2631..3395
                     /locus_tag="VFV99_07430"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5059175.1"
                     /translation="MRVRCLALALLALVACSKGEQKQQAKPKPEQKPEPVEVKPPDVY
                     AEARFEMVERTIVDRGIKDDRVIAAMKMTPRHEFVPPDIRGRAYDDSPLPIGFDLTIS
                     QPYIVAFMTEAVKVKAGDKVLEIGTGSGYQAAVLAMMGAKVYTIELHDELARRTKLVL
                     QKLGFKNVSMKQGDGYFGWADAAPFDAIMITCATPEIPPPLLAQLKPGGRLIAPIGDD
                     YSQELMVFTKTGDDLERQPLMEVRFGLMKGEINKPR"
     gene            3392..4174
                     /locus_tag="VFV99_07435"
     CDS             3392..4174
                     /locus_tag="VFV99_07435"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5059176.1"
                     /translation="MTRALLVANLGLLGLLACSAEKSSPPPPQQPAPAPAPTPTRPVD
                     TYVAERNKMVDETIVSRGVKDDRVIAAMRKVKRHELLPPSLRPRAYEDNPLPIGFEQT
                     ISQPYIVATMSEAAQLTPGEKVLEIGTGSGYQAAVLAELGADVYSIEIVEPLATRTHE
                     LLKRMGYFDKMHLRIGDGFRGWPEAAPFDAIIVTAAPEKIPQPLIDQLAVGGRLVIPV
                     GKDGDQDLRVVTRSKDGTTSETLFPVRFVPMTGEAQATQPHR"
     gene            complement(4449..5198)
                     /locus_tag="VFV99_07440"
     CDS             complement(4449..5198)
                     /locus_tag="VFV99_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059177.1"
                     /translation="MLVAACSGEIQSDIYDDLNPKDQVALEKWVEKALPVLGAKCVMC
                     HDGSRPEPIGYLVGATDIEKRDTLIAYMPAVINLNAPMSSRMLTKGEHTGPALEAKEA
                     SDILEWIKAEKESRGSTAVPIRSDKAAPMLCNGLPAPCPLNTIDLMTLGQPGTLTFEA
                     TQLSADLYLTSIKIKAGAAGLHITHPIFESWPAGATEPKPDSIDRWFNVDLQIAPDAE
                     VPIGTGEGSFAGFAGADPLSIRFDVYEMKTM"
     gene            5416..8166
                     /locus_tag="VFV99_07445"
     CDS             5416..8166
                     /locus_tag="VFV99_07445"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5059178.1"
                     /translation="MNSLLPTTIEGASAGCELVTSDGRALALVSATLRGEAQGGLARL
                     VLEQRFENPYSDTLKVTYRMPLPADGAISGYEFVIGERVVKGQIDKKRQARERFERAI
                     ASGRTAALLEQERADIFTQQIGNIPPREAVLARITIDQRLVWLPEGEWELRFPTVIGP
                     RYIGSADTAEDVRATHIKVTDKALPVTIQIEIAVRDVIVAGAKPSSPSHLIERRGDVI
                     ELKSGSRLDRDIVVRWPVATPTVGLSLASARPQSGNDAYALLTIVPPAKEAHPVAVPR
                     DLIVLLDTSGSMSGGPLDKAKQVVAMMIDSLDERDRLELIEFSNSPRRYLTEPVVATA
                     AAKQAAINWVRSRIADGGTEMRAGVIEALQTLRVGAQRQVVVVTDGYVGGEQQILEAL
                     NRRLPKSCRLHVLGVGSAVNRSLATSLARAGRGIEVIVGLDEDAERGAKRLLDRTRSP
                     MLTNVEVSGSAVLRHAPAQMPDCFEGAPLVCALAIKPEGGEILVRGQGARDTWQQTIK
                     VKPLAQGEGNQAIVALYGRERVADVEANALFSSVDGEVEDLGLTFQIATRMTSWVAID
                     EIRRSVSFTREQIVPQELPYGTSAAAFGLRGAAPAASMTRAGSASAASMRMLESMAKV
                     DVNEFRQEESTARSLSIDPASFADVGESGFDADDDYSDLAAGAPSEMSESPPAPAYSR
                     RPSPPAPGYSRPMGPGAGAPPAGRPAPGPRSASTTTRSDLPSRPQAPEADFETEEPTD
                     PVMKESEPEERSIAPMSRAPMSRAISSAPMAQPPSAPRPAAAAPIAQPPSAPRPTEAA
                     PMPKPEAKPTPELAKAKTAMAPEPEPKADEPARARRSTSQGVGAPSAPSGGAPSAPSG
                     GPPPSAGYPQLRDLQLHAAPEKAELQAVRGKQPAKKPPVLWIAIAVLLALIALLIWWL
                     AA"
     gene            8163..8708
                     /locus_tag="VFV99_07450"
     CDS             8163..8708
                     /locus_tag="VFV99_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059179.1"
                     /translation="MIDLDVHLAAITVGDTAAFARWLAGAEGPVRESLRSFATVVDVE
                     AVLQEALLRVWQVAPKFVPDGRPNGLLRLGVRIARNLAVSEVRRTRATPVEPGDLEVA
                     LERAPDEPDPMLRKAIVDCREKLPAKPRQAFDSRLASAGGQDDVELAEALGMKLNTFL
                     QNFTRARNLLVDCLKKKGIAL"
     gene            8705..8941
                     /locus_tag="VFV99_07455"
     CDS             8705..8941
                     /locus_tag="VFV99_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059180.1"
                     /translation="MTREEEDLLVEQVASAHRPRALDELRYHPAWHDIDAAARERAFD
                     LARALRPLEAALDEDGLSTTARAVLNKIVSARTE"
     gene            9184..>9529
                     /locus_tag="VFV99_07460"
     CDS             9184..>9529
                     /locus_tag="VFV99_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5059181.1"
                     /translation="MVLVPLLAACSTEFDPKQCAEDSECGAGSVCELRAAVPVCVHAE
                     DAPLLIGESAPISGTNQALGTGMKLGIELAFEEQNAMGGIRGRKLELVFRDDAYQPQL
                     AEQAARTLVDAQV"
BASE COUNT         1651 a         3104 c         3139 g         1635 t
ORIGIN      
        1 tcacccgcat ctggttcgcg gccttgggct gcaacgcagg cgcgagcacg cggctgtcgc
       61 tacacgccac caacagcacg agccagaccc accgcatcgt tagttagacg cggcgtcggc
      121 gagccggttg ctcgcttctc agcgttcttc gcggcggccc ggcgcccact cgacgatctt
      181 gtcggtgcga gtctcgagga atcgcgcgag cttgtgatag tcgctgccgg tcttgatgaa
      241 gcgaacctcg gtcttgaggt tcgtcgggtt gatcaggtcc ttgaacggta cgtagttgag
      301 atcgagctgg ccactcacgc tgaccatgtg gccatcgagg ttctcctcga cgagcgcgcg
      361 atacgcaccg atgccgagct gactgccgag catcacgtcg aacgcatgcg gcggcgcgca
      421 gcgcgactcg tagccgagct gcaggcccgt cagcttcttc ttgcggccgg tgcgctgctc
      481 gtggcgcttg ctgacgagct gtgcgaccag cttgccgaga tcgaagcgac cgagcggcaa
      541 gtggccgtgc tcgtcgagtg gcaggtttgc gatctgcgtc tcgggcaaca tctcggcgag
      601 gccttcggcg agcacgacgg tgccgtagta cttgccgcgc ttttcgcgcg cgatgacgag
      661 atcgacaatg cgatcgacga gcgcgtcgag cgataggtgc ttgttcttgc cttccactgt
      721 gagcggaccg gtgatgtcct cgaccgcgag cacgaggttt gcctcacctg cgatcgcgac
      781 gccgtacgac agccagccgg ccttgcggcc catcgtctcg acgatgaagt agctgctcgt
      841 cgcgatcgcg tcggcgcgga ggttctgcag ctccttcgcc atcacgtcga cggccgtgaa
      901 gaagccgaac gtgaagtcga tgccacggta gtcgttgtcg atcgtcttcg gcagatggat
      961 gacctgcacg cggcgcgcat cgggcggcag ccgcttctgg tactcgtaga gaaagttcgc
     1021 cgtcttcagt gtgtcgtcgc caccgatcga gaccagcgca tcgatctcga gatcgaccat
     1081 cgcgttgtag acgttgcgca gcttcttcgt cttgttcaga tcgtcgaggt ccgccgcctt
     1141 ctcgatgccc ttaccgggat tgctgcgcgc ggtaccgatc acgatgccgc gcgtgttgcg
     1201 cagaccggag agatccttca gctcgaagat ccggtagtgc tcgtctggga gcagacggtg
     1261 cgagaccggg tggtactcct cgagattgga gtagccgtgg aagaagccga tgacttcgcg
     1321 gctgtcttcg agaaacgaaa tcgcagccga cgagatcacg gcattcgcgg ccggcgccgg
     1381 accaccggag aacaccaatc cgacacgttt gatgtggtcg cccttacggc tcatttccgg
     1441 ggagcctacc cgattacttc tgcttcggcg ccctgcccat gaggatcaaa tgcccgcgcc
     1501 acttcttatc cgggtagccg aaccgaaacg tgagaggccg cttcggttgt tgcttgtaga
     1561 gcgcgaccca ctgcggcgtg acttcaccgc cggccttcat gttcgacgcc tcgtagctac
     1621 cgtaagtctc gtactcgaaa ccggctggtg tgccgtactt cggcggcaat cccgtcgagt
     1681 ccgacaccat ccagtcgacg tgatcgatga tgtacttgcg catcgcctgg aactcgccga
     1741 acgacagtaa gtaactcgcc gccttcgtca tcgctgcgac gttgcccttc ttctcgaggt
     1801 gcacgagcgc gtttggctcc gccttcagat gcttgtcgtc gaggttcgcc atgatgtggc
     1861 gatagatttg ctcgcgcgtg ccgccctgct tcttgaactt gatttcgacg ttcgagagct
     1921 gctggttgcg cttgccgacg tcctcgatct tgtcgatcgc cgcgaagtcc gcgtcgctga
     1981 ggtactcgat atcgccgtct gtcgtcagcc ggaagtagcg catcgccacc ggctcgtagc
     2041 cgtgaatcgt cagcgccgag aggctgaaga tcagctgcgt cggcaactgc gcgccacgca
     2101 tcgcgccgat catgtccatc gtcttcgagt agctcgagcg gtacagcgac gacagctcct
     2161 tctcgacggt cttgagcgca accctgacct gcttgtcatc catgcgcgac agcgcgcgcg
     2221 gatcgcccgc gggctcgagc gacagtgtcg tgatctcgct ggcgtccgga tagaccgtca
     2281 gcgccgtcga cagatcgccg cccgcaaacg ggtagacgac aatcttcggc acggttgccg
     2341 gtacgtgcgc ggcgaagaat tcgcgaaccg gcgtgaccca gccggtcttg taggcgtctt
     2401 gcgctttgcg gatggtcttg cagtgggcct cgacggtctt tgcggtgacc gtcgccggcg
     2461 gggtcccgtc ggcgcatgcg ccaaccacga gcaatgcctt cgcctcgtcg agcagctcgc
     2521 gcggtttgtc ggcacgggca ttcgcccccg aagcgaccac ggcggccacc actacccaga
     2581 cctgcatgaa acgcatggcc gcaatctgcc acagcgtgta gccttcgcgc atgcgggttc
     2641 ggtgtcttgc gctggcattg cttgcactcg ttgcgtgctc caaaggtgag caaaagcagc
     2701 aagccaagcc caaacccgag caaaagcctg aacccgtcga ggtaaagccg ccggatgtct
     2761 acgctgaagc gcgcttcgag atggtcgagc gcaccatcgt cgaccgcggc atcaaggacg
     2821 atcgcgtgat cgccgcgatg aagatgacac cgcgccacga gttcgtgcca cccgacattc
     2881 gcggccgcgc ctacgacgac agcccgctac cgatcgggtt cgacctcacg atcagccagc
     2941 cgtacatcgt cgcgttcatg accgaagccg tgaaggtgaa agccggcgac aaggtcctcg
     3001 agatcggaac cggcagcggc taccaggccg ccgtgctcgc gatgatgggc gcgaaggtct
     3061 acacgatcga gctgcacgac gagctcgcgc gccgcacgaa gctcgtgctc cagaagctcg
     3121 gcttcaagaa cgtgtcgatg aagcaaggcg acggctactt cggctgggcc gatgccgccc
     3181 cgttcgacgc gatcatgatc acgtgcgcga cgccggagat tccgccgccg ctgctcgcac
     3241 agctcaagcc cggcgggcgc ctcatcgcgc cgatcggcga tgactactcg caggagctga
     3301 tggtgttcac gaagaccggc gacgacctgg agcgccagcc gctcatggaa gttcgcttcg
     3361 gtctgatgaa gggcgagatc aataaaccgc gatgacccgc gcgctgctcg tcgcaaacct
     3421 cgggctcctc gggctcctcg cgtgcagcgc ggagaagtcc tcgccgccac cgccgcagca
     3481 gccggcgcca gcacccgcgc caacgccaac acgtcccgtc gatacgtacg tcgccgagcg
     3541 caacaagatg gtcgacgaga cgatcgtctc gcgcggcgtg aaggacgacc gtgtgatcgc
     3601 agcgatgcgc aaggtcaagc gtcacgagct gctgccgccg tcgctgcggc cacgcgcgta
     3661 cgaggacaat ccactgccga tcggcttcga gcagacgatc agccagccgt acatcgtcgc
     3721 gacgatgagc gaagccgcgc agctgacgcc gggcgagaag gtgctcgaga tcggaacggg
     3781 tagcggctat caggcggcgg tgctcgccga gctcggtgcc gacgtctact cgatcgagat
     3841 cgtcgagcca ctcgcgacgc gtacgcacga attgctcaag cgcatgggct acttcgacaa
     3901 gatgcacctg cgcatcggcg atggctttcg cggctggccg gaggcggcgc cgttcgacgc
     3961 gatcatcgtg acggcggcac cggagaagat tccgcagccg ctgatcgatc aactagcagt
     4021 gggcggccga ctcgtgattc ccgtcggcaa agacggcgat caggatctgc gcgttgtcac
     4081 ccgcagcaag gacggcacga cgagcgagac gctgttcccc gtgcgcttcg tgccgatgac
     4141 gggcgaagcg caggcaacgc aacctcatcg ctgacatggt cgccgccctg catcggcgag
     4201 agcagatccc tatatgtggt gggtctcctg tgccgaagtg gtacgaacgc cgttagtacg
     4261 tggcatcgcg atctcgcatc cgcgcgtgcc gtactaaccg tgtacgtacc acttcggcgg
     4321 gggacaccct ctgcatatgg ggatctgtcg cgacgaaggt gcgtcgcctc tggacgcacc
     4381 gctcttcgct ggtcaccgcc gtcgccgtcg tcgctcgtcg ccgtcaatgt ccacgacggc
     4441 gcgggtatct acatcgtctt catttcgtag acgtcgaacc ggatgctgag cgggtcggca
     4501 ccggcgaaac cggcgaacga gccctcgccg gttccgatcg gaacttcggc gtcgggcgcg
     4561 atctggagat cgacgttgaa ccagcgatcg atcgaatccg gcttcggctc ggtcgcaccg
     4621 gcgggccacg actcgaagat cgggtgcgtg atgtgaaggc ctgctgcgcc tgctttgatc
     4681 ttgatgctcg tcagatagag atccgccgac agctgcgtcg cctcgaacgt gagcgtgccg
     4741 ggctggccga gcgtcatcag atcgatcgtg ttgagcgggc acggagcagg taggccattg
     4801 cacagcatcg gcgccgcctt gtcgctgcga atcggcaccg ccgtgctgcc gcgcgattcc
     4861 ttctccgcct tgatccactc gaggatatcg gacgcttcct ttgcttcgag tgccggaccg
     4921 gtgtgctcgc ccttcgtgag catgcggctc gacatcggcg cgttgaggtt gatcaccgcg
     4981 ggcatgtacg cgatcagcgt gtcgcgcttc tcgatatcgg tcgcgccaac gagatagccg
     5041 atcggctccg gccgcgagcc gtcgtggcac atcacgcact tcgcgccgag cacgggcagc
     5101 gccttctcga cccacttctc gagtgcgacc tgatctttgg gattcaggtc gtcgtagatg
     5161 tcgctctgga tttcgccgct gcacgcagcg acaagcaagg cgatcgcgat agacgctaaa
     5221 cgaaccaccg gcgccgatgt taccgcagcc gccgccggcc gcgggcctga ccgattctga
     5281 cttctcggtc gccgcctgtg atggaagtaa cgggaacaag gggcgaaaat tccttgtcac
     5341 taaacccaca cggcttgcgt tggtcggctg aaaggaccac cgaccatgct gctttcgcta
     5401 ccgtctgcgc ctgccgtgaa ttctctgctg ccaaccacga tcgagggggc ctccgcgggc
     5461 tgtgagctcg tcacgagcga cggccgcgcg cttgccctcg tttcggcgac cctgcgcggt
     5521 gaggcgcagg gcgggctggc ccggctcgtg ctcgagcaac ggttcgaaaa cccgtatagc
     5581 gacacgctga aagtcacgta ccgcatgcca ctgcccgctg acggtgcgat cagcggctac
     5641 gagttcgtga tcggcgagcg cgtcgtcaaa ggtcagatcg acaagaagcg tcaggcgcgg
     5701 gagcggttcg agcgggcgat cgcctccggt cgtaccgcgg cgctgctcga acaagagcgc
     5761 gccgacatct tcacgcagca gatcggcaac attccgccgc gcgaagcggt cctcgcgcgg
     5821 atcacgatcg atcagcggct cgtgtggctg cccgaaggcg agtgggagct gcggttcccg
     5881 accgtgatcg gcccgcgcta catcggctcg gcggataccg ccgaggacgt gcgcgcgacg
     5941 cacatcaagg tcaccgacaa ggcactgccg gtgacgatcc agatcgagat tgccgttcgc
     6001 gatgtgatcg tggccggcgc gaagccgagc tcgccgtcgc atctgatcga gcggcgcggc
     6061 gacgtgatcg agctcaagag cggctcgcgt ctcgatcgcg acatcgtcgt gcgctggccg
     6121 gtcgcgacgc cgaccgtcgg gctgtcactc gcgagcgcac gcccgcagag cggcaacgac
     6181 gcatacgcgc tgctcacgat cgtgccgccg gccaaggagg cgcacccggt cgcggtgccg
     6241 cgcgatctga tcgtgctgct cgatacgagt ggctcgatga gcggcggtcc gctcgacaag
     6301 gcgaagcagg tcgtggcgat gatgatcgac tcgctcgacg agcgtgaccg cctcgagctg
     6361 atcgagttct cgaacagccc gcgccgttac ctcacggagc cagtcgtcgc aacggcggcg
     6421 gcgaagcagg cggcgatcaa ctgggtgcgc tcgcgcattg ccgatggcgg caccgagatg
     6481 cgcgccggcg tgatcgaagc gctgcagacg ttgcgcgtcg gtgcgcagcg ccaggtggtc
     6541 gtggtcaccg acggttatgt cggtggcgag cagcagatcc tcgaggcgct caatcgccgg
     6601 ctgccgaagt cgtgccggct gcacgtgctc ggcgtcggct cggcggtcaa tcgctcgctc
     6661 gcgacgtcgc tcgcgcgtgc gggacgcggc atcgaagtga tcgtcggcct cgacgaggat
     6721 gccgagcgcg gcgcgaagcg tctgctcgat cgcacgcgct cgccgatgct gacgaacgtc
     6781 gaggtgagcg gtagcgcggt gctccgtcac gcacccgcgc agatgccaga ctgcttcgag
     6841 ggtgcgccgc tcgtgtgcgc gctcgcgatc aagccggaag gcggcgagat cctcgtgcgc
     6901 ggccagggtg cgcgcgacac ctggcagcaa acgatcaagg tgaagccgct cgcccagggc
     6961 gaaggcaacc aggcgatcgt cgcgctatat ggccgcgaac gcgtcgccga tgtcgaagcg
     7021 aacgcgctgt tctcgagcgt ggacggcgaa gtcgaggatc tcggcctcac gttccagatc
     7081 gcgacgcgca tgacgtcgtg ggttgcgatc gacgagattc gccgctcggt cagcttcacg
     7141 cgcgagcaga tcgtgccgca ggagctgccg tacggcacga gcgccgccgc gttcggtctg
     7201 cgaggtgcag cccccgccgc gtcgatgacg cgagctggat cggcgtcggc agccagcatg
     7261 cggatgctgg agagcatggc gaaggtcgac gtcaacgagt tccgccagga ggaatcgacc
     7321 gcgaggtcgc tgtcgatcga tcccgcatcg ttcgccgacg tcggcgaatc agggtttgac
     7381 gcggacgatg actacagcga cctcgccgcg ggcgccccga gcgaaatgtc cgagagcccg
     7441 ccagcgccag cgtactcgcg ccgtccatcc ccgccagcgc ccggctatag ccgcccgatg
     7501 gggccaggtg cgggtgcgcc gccggccgga cgtcctgcgc cgggaccacg ctcggcgagc
     7561 acgacaacgc gaagcgatct gccatcccga ccgcaagcgc cggaagctga cttcgagacc
     7621 gaggaaccga ccgacccggt catgaaggag tccgagcccg aggagcggtc catcgcgccg
     7681 atgtcacgcg caccgatgtc acgcgcgatc tcgagcgcgc caatggcaca gccgccgtcg
     7741 gcaccgcgac cggccgcagc cgcgccgatt gcacagccgc cgtcggcacc gcgaccgacc
     7801 gaagcggcgc cgatgccgaa gccggaggcg aagccgacgc cggagctggc gaaggcgaag
     7861 acggccatgg cgcccgagcc cgaaccgaag gccgacgagc ccgcgcgtgc gcgacgctcg
     7921 acgtcgcaag gtgtcggcgc accgtccgca ccgagcggag gtgcaccgtc tgcaccgagt
     7981 ggtggtccgc cgccgagtgc cggctatccg cagctgcgcg atctgcagtt gcacgcggct
     8041 ccggaaaaag ctgagctgca agcggtgcgc ggcaagcagc ccgcgaagaa accacccgtg
     8101 ctgtggatcg cgatcgccgt gctgctcgcg ttgatcgccc tgctcatctg gtggctagcg
     8161 gcgtgatcga tctcgatgtc cacctcgctg cgatcaccgt cggggatacc gccgcgtttg
     8221 cgcgctggct cgccggtgcc gaaggtccgg tgcgcgagtc gttgcgctcg ttcgcaaccg
     8281 tggtggacgt cgaagcggtg ctgcaagagg cgctgctgcg cgtgtggcag gtcgcgccga
     8341 agttcgtgcc ggatggccgg ccgaacgggt tgctccggct cggcgtgcga atcgcgcgca
     8401 acctcgcggt gagcgaagtc cgccgcacgc gggcaacgcc cgtcgagccg ggtgatctag
     8461 aggtggcgct cgagcgcgcc cccgacgagc ccgatccgat gctgcgcaaa gcgatcgtcg
     8521 attgtcgcga gaagctgccc gcgaagccgc gacaagcgtt cgatagccga ctcgccagcg
     8581 ctggcggcca ggacgacgtc gagctcgccg aagcgctcgg catgaagctg aacacgttcc
     8641 tgcagaactt cacgcgcgcg cggaatctgc tggtcgactg cctgaagaag aaaggcatcg
     8701 cgctatgacg agagaagaag aggacctcct cgtcgaacaa gtcgcgagcg cgcaccgacc
     8761 gcgtgcgctc gacgagcttc gctaccatcc ggcgtggcac gacatcgatg ccgccgctcg
     8821 cgagcgcgcg ttcgacctcg cgcgcgcgtt gcgtccgctc gaagcggcac tcgatgaaga
     8881 cggtttgtcg acgacggcgc gagctgtgct gaacaaaatc gtgagcgcgc ggacagagta
     8941 gatcccctct tgatccgtta cacgcgaaac gtcgtcgttg gggtagcgcc gacgagccga
     9001 gatcgatttc tcgacgacga tcgcggttct ccctgactct gcccgttgcg atcctcacag
     9061 acgcgaaagc tctcgaattg cttttgagta attcgcgttg cgaatcgcga aggtgtggac
     9121 cttagtaacc cttgcgtccg acacgttgtg ctgtgcaacg gtgcagttgc aatgaagggt
     9181 ttgatggtat tggtccctct cctcgcggcc tgctcgacgg aattcgaccc gaagcagtgc
     9241 gccgaagata gtgaatgcgg agccggcagc gtctgcgagc ttcgcgctgc ggttccagtc
     9301 tgcgtccacg ctgaagacgc gccgctgctg atcggcgaga gcgcaccgat cagcggcacg
     9361 aaccaggcgc tcggcaccgg catgaagctc ggcatcgagc tcgcgttcga agaacagaac
     9421 gcgatgggcg gcatccgcgg ccgcaagctc gagctcgtgt tccgcgacga cgcgtatcag
     9481 ccgcagctcg ccgaacaggc cgcgcgcacg ctcgtcgacg cgcaggtct
//
LOCUS       DASUFX010000187         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_167247,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000187 DASUFX010000000
VERSION     DASUFX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167247"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFV99_07465"
     CDS             complement(<1..272)
                     /locus_tag="VFV99_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059182.1"
                     /translation="MQQPWIDLSTGINPRSYPAPRASQRSRNRLPEPTELARLEALAA
                     AAFGVTDPACVVATAGTECALRLLPQLVNLKAAVIVGPTYGSHADA"
     gene            complement(358..2268)
                     /locus_tag="VFV99_07470"
     CDS             complement(358..2268)
                     /locus_tag="VFV99_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5059183.1"
                     /translation="MLSCLLAPAGAVFGADAPKQNELERVVVTANREAQPLSHVGDSV
                     TLIEPAEVRASQKLAISDLLATTPGITVSRNGGLGTSTQLRIRGAESDQTVVLIDGVK
                     LNDPSSAGGGFDFGNLLTGDYMRIEVLRGPQSTLWGSQAIGGVINVVTTVPEGPLLAE
                     YSAEGGERGSATVRARAEAGGERFAWRVAGQYLTTDGVSALNDDRGGHEKDGYRNVGA
                     NVRGLWRITDAVSAELRTTYSSGRADFDGFPAPNFELTDTREYSDTDELVAYTGVNAA
                     MFAGRLQNRFGFAYTDTDRENIDPHSPVPTTFDASGSNQRWEYQGTLTLTDRIGGVFG
                     LESERSELDTRSPSVFDPNPVPLVHDARLDSIYAQLTVAPIETLTLTGGMRYDDHDTF
                     GSESTGRATLAWAATPTTIVRATYGEGFKAPTLFQLFSDFGNEDLAPERADAWDAGVE
                     QHLLNDALVLSATYFGRNTRSMIDFVSCFPATDPRCATRPFGFYDNVAKTEADGIELG
                     LTAHLGERLRIDANYTDMDTTNRTPGANFGRELPRRPGQVFNGEVTYAWPIGLTTTVA
                     VLDVSRNFDNPSNTVVVDGYTVVDFRASYALRADLELFGRIENAFDEEYETIARYGTP
                     GRGVFVGVRQTF"
     regulatory      complement(2408..>2539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          415 a          832 c          856 g          436 t
ORIGIN      
        1 caagcgtccg catgcgatcc gtacgtcggg ccgacaatga cggctgcttt gagattcacg
       61 agctgcggca agagtcgcag cgcgcactcg gttccagcag tcgcgacgac acacgcagga
      121 tccgttactc cgaacgccgc cgctgcgagt gcttcgagac gcgccagctc cgtcggctcg
      181 ggcaatcgat tgcgcgaacg ctgcgacgcg cgcggtgccg gatacgaccg cggattgatc
      241 cccgtcgaga ggtcgatcca cggctgctgc acatcgggaa acaaagagcg cgcaacgccg
      301 agccgcccgc cgtgatgcgc gaagcggccc gtcgcgattt gcgtttcgct gtgcacgtca
      361 gaacgtctgg cgtactccga cgaacactcc gcgccccggc gtgccgtagc gcgcgatggt
      421 ctcgtattcc tcgtcgaacg cgttctcgat ccggccgaac agctccaggt cggcgcgcag
      481 cgcgtacgac gcgcggaaat cgacgaccgt atagccgtcc acgacgacag tgttcgacgg
      541 gttgtcgaag ttgcgactga cgtcgagcac tgcaacagtc gtcgtcaggc cgatcggcca
      601 cgcgtacgtg acctcgccgt tgaacacctg tcccggacga cgcggcagct cacgaccgaa
      661 gttcgcgccg ggcgtgcgat tggtcgtgtc catgtcggtg tagttcgcgt cgatgcgcag
      721 acgctcgccg aggtgcgcgg tgagcccgag ctcgataccg tcggcctcgg tctttgccac
      781 gttgtcgtag aagccaaaag gccgtgtcgc acagcgtggg tcggtcgccg ggaagcacga
      841 gacgaagtcg atcatgctgc gcgtgttgcg gccgaagtac gttgccgaca gcaccagagc
      901 atcgttcagc aggtgttgct cgacaccggc atcccaggcg tctgcacgct ccggcgcgag
      961 gtcctcgttg ccgaagtcgc tgaacagctg gaacagcgtc ggcgccttga aaccttcgcc
     1021 atacgttgcg cgcactatgg tcgtcggcgt ggcggcccac gcgagcgtcg cacggccggt
     1081 gctctcgctg ccgaacgtgt cgtggtcgtc gtagcgcatg ccgccggtga gcgtgagcgt
     1141 ctcgatcggc gcaaccgtga gttgcgcgta gatgctgtcg agccgcgcgt cgtgtaccag
     1201 cggcactgga ttcggatcga acacgctcgg cgagcgggta tcgagctcgg agcgctcgga
     1261 ttccaggccg aacacgccgc cgatcctatc ggtgagcgtc agcgtgccct ggtattccca
     1321 gcgctggttg ctgccgctcg cgtcgaacgt cgtgggtacc ggcgagtgcg gatcgatgtt
     1381 ctcgcgatct gtgtcggtgt acgcgaagcc gaagcggttc tgcagccggc cggcgaacat
     1441 cgcggcgttg acgccggtat acgccacgag ctcgtcggta tcgctgtact cgcgcgtgtc
     1501 ggtgagctcg aagttcggtg ccggaaagcc gtcgaagtcg gcgcgtccgc tgctgtaggt
     1561 agtgcgcagc tcggccgata ctgcatcggt gatgcgccac agtccgcgca cgttggcgcc
     1621 gacgttgcga tagccgtcct tctcgtgacc gccgcgatcg tcattcagtg cggagacacc
     1681 gtcggtcgtg aggtactggc ccgcgacgcg ccaggcgaag cgctcgccgc cggcttcggc
     1741 gcgtgctcgt accgtcgcag agccgcgctc gccaccttcc gcggagtatt cggccagcag
     1801 cggtccttca ggaaccgtcg tgacgacgtt gatgacgccg ccgatcgcct ggctgcccca
     1861 cagcgtcgat tgcgggccgc gcagcacttc gatgcgcatg tagtcgccgg tcagcaggtt
     1921 gccgaaatcg aaaccgccgc cggccgagga cggatcgttg agcttcacgc cgtcgatgag
     1981 cacgacggtc tggtcggact ccgcgccgcg gatacgcagc tgcgtcgagg tgccgaggcc
     2041 gccgttgcga gacacggtga tgccgggtgt cgtcgcgagc agatccgaaa tcgccagctt
     2101 ctggctggcg cgaacctccg cgggctcgat cagcgtcacc gagtcgccga cgtgcgagag
     2161 cggctgcgcc tcgcggttcg cagtgacgac gacgcgctcg agctcgttct gtttcggcgc
     2221 atcggcgccg aatacggcgc ccgcgggcgc gagcagacat gacaacaccc agggaccaca
     2281 acgcgatgca gcggatgaag cggacatggt gcgaaagctc cggaaagact gccaaacccc
     2341 cggccatctc tggcccggac cggtcgtcgc tcgggaactc ccgctcgatc agcacacccc
     2401 ggctgagcga tggacgacgg cggcaagcag gtctcctggc tcgtggcttg gagtccgtcg
     2461 cgtcaccttc ccggatcggc aattgcctgc agatccagtg gtctcgacgc gcggcgcacc
     2521 acttacagtt gcgggggca
//
LOCUS       DASUFX010000188         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_28710,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000188 DASUFX010000000
VERSION     DASUFX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28710"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFV99_07475"
     CDS             <1..565
                     /locus_tag="VFV99_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5059184.1"
                     /translation="ARGLDRDDPMIHQRIAGRMSYVLEQAIVMPEPTDAELRAWFDAH
                     RDQWSVAEHLDFTHVFIAGSDDAANQRADEIAAALAAGASPDRLGDTFSGGLRYRGRK
                     LTDLALQFGDDFVTGLATQPIGTWVKRRSRHGLHIVRVDRVDAAKPADFAAARLDVRK
                     QWLDARRGQELGVALRRLRDGWTIERK"
     gene            562..1515
                     /locus_tag="VFV99_07480"
     CDS             562..1515
                     /locus_tag="VFV99_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5059185.1"
                     /translation="MKLVAAVAILVALAANADAHDIRPGVLSLTEQSRGDYALHFAPP
                     IDSRGDATEIAIDFPAGCTRNADRLHCDDAIAGTLVIRGMRGAAMKTLVILVRASGER
                     SEWIVDAAAPQITIGASPPSGLFAWIRVGIMHILTGFDHLAFVIGLLLVLDFSLGRRL
                     LVTITAFTLAHSLTLALAVLGVIAVHPAPVEACIALSVLLVAREGTHEQPTVIRRWPW
                     LAAGAFGLIHGLGFATALRELGLPRESLARALVSFNVGVELGQLAVVAVLVALVAAAR
                     RLIDERRVVGRHVHRAACYLLGALAAWWLLDRVVELASGAR"
     gene            complement(1915..3522)
                     /locus_tag="VFV99_07485"
                     /pseudo
     CDS             complement(1915..3522)
                     /locus_tag="VFV99_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
     gene            3674..4984
                     /locus_tag="VFV99_07490"
     CDS             3674..4984
                     /locus_tag="VFV99_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059186.1"
                     /translation="MRDAIATAGIALVTFMLCVLAPLRPLSGDTVPGRIGGAVVRCAG
                     DFDLEHVDWVRTETAHDRLFYWLQRDEAGEHTSVFGPAPAAVMAVALLAFGDGDRIDD
                     DTLRRRERGVAAVLLAIATVLLVLAARARVTLPRALLAGAVCTLSFAGAGTLGQGLWQ
                     ATTALPALTGALATLAWRDRRPRLALATPALLLLAVMLRPTMAPLVLGIGITWARGTP
                     DRRSWLIATVAALVLVAPLIVYNAIHLWSPLPLGQWNANARATQHVFSLADATTGVAG
                     LLVSPARGLLWFAPIVIVGTVLGMRNASYRWIAGGIVLQILAMGMFYLWHGGQAFGPR
                     FLTEATWVGTWLALGTELTARRWLLGVAITVTILVGSLALWRYRPEQWEFPRRPEAHP
                     SLFWNVVDSPIPATFTDPPRGAVRAYDSPPVNGLACERGQLRSY"
     gene            complement(4941..5315)
                     /locus_tag="VFV99_07495"
     CDS             complement(4941..5315)
                     /locus_tag="VFV99_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059187.1"
                     /translation="MPVFKIARFKVRADSKDTAERAMFEFATYVRNELPDSAWTTYRD
                     ASAPLKYMSLIIADDAAAEERYRTAPGTQAFVDALSPSLVGSIEFTDYQLVTSSDLAR
                     RHRDNDRGASRSGAGRVRRRGR"
BASE COUNT          792 a         1911 c         1765 g          960 t
ORIGIN      
        1 ggcccgcggc ctcgatcgcg acgacccgat gatccaccag cggatcgccg ggcggatgag
       61 ctacgtgctc gagcaggcga tcgtgatgcc cgagccgacc gacgccgagc tgcgcgcgtg
      121 gttcgatgcc caccgcgacc agtggagcgt cgccgagcac ctagacttca cgcacgtgtt
      181 catcgccggc agcgatgacg ccgcgaacca gcgcgcggac gagattgctg ccgcactcgc
      241 ggccggtgcc tcgccggacc ggcttggcga tacgttctcg ggcggcctcc gctatcgcgg
      301 gcgcaagctg accgatctcg cgctgcagtt tggcgacgac ttcgtcaccg gcctcgcgac
      361 gcaaccgatc ggcacgtggg tcaagcgccg gtcgcgccac ggcctgcaca tcgtgcgcgt
      421 cgatcgcgtc gacgccgcca agccagcgga ctttgccgcc gcacggctcg acgtccgcaa
      481 gcaatggctg gatgcgcgcc gcggacagga gctcggtgtc gcgctgcgtc gcctccgcga
      541 cggctggacg atcgaacgca aatgaagctc gtcgccgccg tcgccatcct cgtcgcgctc
      601 gccgcgaacg ccgacgcgca cgacattcgc ccgggcgtgc tgtcgctgac cgagcaatcg
      661 cgcggcgatt acgcactgca cttcgcacca ccgatcgaca gccgcggtga cgccaccgag
      721 atcgcgatcg acttccctgc cggatgcact cgcaatgcgg accgccttca ctgcgatgac
      781 gcgatcgcgg gcacgctcgt gatccgcggc atgcgcggtg cggcgatgaa gacgctcgtg
      841 atcctcgtgc gcgcgagcgg cgagcggtcg gagtggatcg tcgacgcagc tgcgccgcag
      901 atcacgatcg gcgcatcgcc gccgtcgggc ctgttcgcgt ggattcgcgt cggcatcatg
      961 cacatcctca ctggcttcga tcacctcgcg ttcgtgatcg gccttctcct cgtactcgac
     1021 ttctcgctcg gccgtcgcct gctcgtcacg atcaccgcgt tcacgctcgc gcactcgctc
     1081 acgctcgcac tcgccgtgct cggcgtcatc gcggtccacc cggcgccggt cgaagcctgc
     1141 atcgcgctca gcgtgctgct cgtcgcgcgc gaagggaccc acgagcaacc gaccgtgatc
     1201 cggcgctggc cgtggcttgc cgccggagcg ttcggcctca ttcacgggct cggcttcgcc
     1261 accgcgttgc gcgagctcgg cctgccgcgc gagtcgctcg cccgcgcgct cgtctcgttc
     1321 aacgtcggtg tcgagctcgg ccagctcgcg gtggtcgcgg ttctcgtcgc gctcgtcgcc
     1381 gccgcgcgcc gactaatcga cgagcgacgg gtcgtcgggc gccacgtcca ccgggccgct
     1441 tgttatctgt tgggtgcgct tgcggcgtgg tggctgcttg accgtgtcgt tgaactggcg
     1501 agcggcgcgc gctgacgcca tcgtcgtgaa gcgatagagc ggcgtcacga tgcgctgacc
     1561 gcagctcgac acctcggcgt accactcgta cgtcttgccg acttcgagac catcgaccgg
     1621 cgtcgcgacc gagtcaggcg ccgcattcgc gaccgtgatg tcgcggaacg gaccgccgat
     1681 gcccttgaga tcgacgtcga gcgtgaactc gctgttcgcg tcggtctcgt acttgtccat
     1741 cgtcggcgag tacgagcgga cggtgaccga ctgcgtcgcg ggcgagaact cccagatgcg
     1801 catgaagccg ccgccgccga ggtcgcggct ctggtagtcc gcgagcatcg agttgatcgt
     1861 gtgaccctcg aacgtgtcga cacgccggct ctctgccgag acgtgaccac cggtcatcag
     1921 ctgcacctgc ggcagatctt tcagcgcgtt gtagatcatc ttgctctgcg cgctgaacgc
     1981 gccgccgccg gtgagcaagt agtgcgtgtt gaggatgccg aacgccttcg ggtgcatctg
     2041 gaagatcgag cgcgcccacg cgatctcggc gggatccgga tcgagctcgt acatgaggct
     2101 gacgacgacg aagtcgagcc cgccccacga gaacgtgatc cagctgttgt cgttcttcgt
     2161 gccgtagtgc ccgccgtagt acgccttccc ctgaaagcga ctgatgccga agaactggtt
     2221 ccacttcgtg gtcgtgagca ccgtgttgga gccggtcagc gtgttgtcgt ggttaccgac
     2281 ctccgcggcc catggcacgc cgtcggggag gccgggctcc ggcttctcga gccgcttcat
     2341 cgccgcatcg gcgacgttcc actgatagag ctgcggctcg ttgttgatca tgtcgccgtt
     2401 gtggatgacg ccgatgatgt tgtagtcgac gcggtgatcg cgcacccact gtgtctggtc
     2461 attgaagtac ttctcgaggt tgcgaccctc gatcgtgtag atctgcgtgt cgggcaagac
     2521 gacgatcgtg aagtcgtcgg cgtccgaaac ctcgcgcacg tgatacgtca cgtcgaccgg
     2581 cgcaacctgc gacatctcga gcgcgacgtc gagtgtgaca tccgggccta ccgcattgcc
     2641 gtctgccggg ctctcggacg cgacgaccgg cgccttgccg tgatcgagct gggcgtcctc
     2701 cgcgacgaac gcaccaccgg ccagcgtcgc cgggaacttg ccgacgctat ccgcggcgac
     2761 cgtgccgtcg ccttcggtat cgaacgacca ccgcgcgacg aggccctcgc ccatcgcgat
     2821 cgtgcgatac atgccgtcgg cgatctcgtc cgcgctgcgc gcgcgattcc acagccgcag
     2881 ctcatcgatc gagccctgca gatggccatg cgccgtggca tcgctggcga tcgcggtcgc
     2941 gatcgcgaac ggatgaatgc tgtcggcgcg cggcgtcgcg ttcgccgtcg ccgtcccgtc
     3001 gagcacgcca tcgaggtaca ggttccacac cttgccgtcg tacgtcgccg cgacgtgatg
     3061 ccactcgccg cgcgccacgc cggtcttgcc catcacggga tggttggcgc cggtcgccat
     3121 gtcttcgaag tcggcgccga gaacatcgcc ccagaagcca aacccgtagt tgcagtcgat
     3181 gttcgtgccc tcgccgcctt cgcccaggcc cttggcggca atcggcacca tcaccagacc
     3241 gccggcgccg gtggtgaacg tcgtgccgtc gccatcgcgc cgcacccacg cttcgatcgt
     3301 gaacgtcgac agcccgagtc cggggtcgac tgcggtcttc gcgtagtcgt cgacgccgtc
     3361 gaaggcgagc gagccaccgg gcgcagaatc gtcggggcac gcgagcggcg tttgcccatc
     3421 gtcgtcgtcg cccttcgcgt gatcgctgca cgcagttgcg aggagcgcca caaaactaac
     3481 aaagcgaatc gcagacatgc ggtcccgatc agcaaggccc atacccacgc ccctccacgc
     3541 aaagctgcgc ggttagcggc gtgaacgacg cgcattccgc tgatcgtcgt ctgcgaggtt
     3601 gcgcacgaac gtttgcagtt gtggcacacg acgcaacacg cgtggtttcg gtggggttac
     3661 aacgtccctc gacgtgcgcg acgccattgc gactgcaggg atcgcgttag tcacgttcat
     3721 gctctgcgtg ctcgcaccgc tgcgaccgct cagcggcgat acggtgcctg gccgcatcgg
     3781 cggcgcggtc gtgcgctgcg ctggcgactt cgacctcgag catgtcgatt gggtacgcac
     3841 cgagaccgcc cacgatcgat tgttttactg gctgcagcgc gacgaggccg gcgagcacac
     3901 ctcggtgttc ggccccgcgc cggccgccgt catggcggtc gcgctgctcg ctttcggcga
     3961 cggtgaccgc atcgacgacg acacgctcag gcgacgcgag cgaggagtcg cggcggtgct
     4021 cctcgcgatc gcaacggtct tgctcgtcct cgctgctcgc gcacgtgtca cgttgcctcg
     4081 agcgctgctc gccggcgcgg tatgcacgct gtcattcgcc ggtgcgggca cgctcggcca
     4141 aggcctgtgg caagcgacca cggcattgcc cgcattgacc ggcgcgctcg cgacgctcgc
     4201 ttggcgcgat cgtaggccgc gactcgcgct cgcgacgccg gcgctgctgc tactcgccgt
     4261 gatgctgcgg ccgacgatgg cgccgctcgt gcttggaatc gggatcacgt gggctcgcgg
     4321 cacacccgac cggcggtcgt ggctgatcgc gacagtggct gcactcgtgc tcgtcgcgcc
     4381 actcatcgtc tacaacgcga tccacctgtg gtcgccgctg ccgcttggcc aatggaacgc
     4441 gaatgcgcgc gcgacccagc acgtgttctc gctcgctgat gccacaaccg gcgtcgccgg
     4501 acttctcgtc agcccggcgc gcggcctctt gtggttcgcc ccgatcgtga tcgtcggcac
     4561 cgtgctcggc atgcgcaacg cgagctatcg ctggatcgca ggcggcatcg tgctccagat
     4621 actcgcgatg ggaatgttct acctatggca cggtggccag gcgttcggcc cgcgcttcct
     4681 caccgaggcc acctgggtcg gcacgtggct cgcgctcggc accgagctca cggcacggcg
     4741 ctggctgctc ggcgtcgcga taacggtgac gatcctcgtc gggtcgctcg cgctatggcg
     4801 ctaccgcccc gagcagtggg agttcccccg ccggcccgaa gcacatccaa gcctgttctg
     4861 gaacgtcgtc gattcgccga ttccagcgac gttcaccgat ccaccccgtg gcgccgtgcg
     4921 cgcctacgac tcgccgccgg tcaacggcct cgcctgcgaa cgcggccagc tccgctccta
     4981 ctagccccgc gatcgttgtc gcggtgacgc cgcgccaggt cgctcgaggt cacgagctgg
     5041 tagtcggtga attcgatcga accgacgagc gacggagaca gcgcgtcgac gaaggcctgc
     5101 gtgcccggcg cggtgcgata acgctcctcg gccgctgcgt cgtccgcgat gatcaacgac
     5161 atgtacttca gcggcgcgct cgcatcgcga tacgtcgtcc acgccgagtc cggcagctcg
     5221 ttgcgcacgt acgtcgcgaa ctcgaacatc gcgcgctcgg ctgtgtcttt ggaatcggct
     5281 ctaactttga atcgcgcgat cttgaacacc ggcatccggc gacgctaccg ctgctcacgc
     5341 ggcgacgcca ggctgcgtct cgctcggcgg cgtcggtgcg aactgcggca atacgagcca
     5401 cttgtgcatc acgcgcgcgg gctcgacc
//
LOCUS       DASUFX010000189         4399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1002126, whole genome shotgun sequence.
ACCESSION   DASUFX010000189 DASUFX010000000
VERSION     DASUFX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002126"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV99_07500"
     CDS             <1..189
                     /locus_tag="VFV99_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059188.1"
                     /translation="EDVLARRVPLLLVSRDQGLGVSERVAQRLAAILGWDAALLGQML
                     DEYKAEVELSRRWRSERA"
     gene            194..649
                     /locus_tag="VFV99_07505"
     CDS             194..649
                     /locus_tag="VFV99_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059189.1"
                     /translation="MTGLLASYGIYGGSLIVAFIAGLFPLLSIEVFLVLVSSVAKPTL
                     TELTMCCALAALSHQVAKTATYYAGVGALEHGKLKEKIEANRERIERWNKAPLLVLAL
                     GGAFGFPPLWVLGFIARPLMRINIAAFTVIVLVTRFGRFFVLAVIPMLF"
     gene            complement(656..1513)
                     /locus_tag="VFV99_07510"
     CDS             complement(656..1513)
                     /locus_tag="VFV99_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5059190.1"
                     /translation="MSRVTQPPSSDDRDVEIVDPFEDDQRTPVLAVGTRDELAEKARE
                     AARRAAAPSVPNEDVAALAHDLKNPLTIIMLEANQIEQRLALRLTPAVARGLERIAQN
                     ASYIDRLLSDLLDMSAADSGRLELRLDRIDLSRLLRDAVGRSVPTQDRDHVRLEIRDV
                     LFVYGDENRLERVVSNLVHNALKYGGDDGGITVRLDARGANACVSVIDSGPGLNADEA
                     RVVFDRYRRTKRAATQSEGYGLGLYTSRKIIEAHKGRIGVISQPGQGARFYFELPAIA
                     PHHMRPNDR"
     gene            complement(1598..2953)
                     /locus_tag="VFV99_07515"
     CDS             complement(1598..2953)
                     /locus_tag="VFV99_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059191.1"
                     /translation="MRLLVLVALLVACKPKEVPYKPPPKDDAPAKPIDAPPEPDAGAP
                     PPETKAQKVVVGDHTSCALMADATVSCWGRNNEGQLGNGTTNDSATPTTVKLHAVKDI
                     VMGASHVCALLDDESVTCWGRIYFGRKENLLSPTAVPGVAKAKRLFAVASASCATLED
                     HSLVCWGDINAAGHLKLSGGSMNRVPTPSNGLDHIAAVTANGALAEDGTVWFWDASGR
                     PQKTAMTNVIEIASSGDEVCGLRRDGSVACAGPATRCAAAAPKTPKPAPKAAPKPAPK
                     KAPAKKPAAKQTAKKTPKGKPAAKPAAKPAAPKPEETAAKGMPIEVLRLPAAKHLAFD
                     VGLCVVTKTGKLECLAPKDGCTVDTPWPGLASVDYVTGNCARITDGSARCWAVDTKSR
                     LVTAIAGVGAALSIAASPSHACAVLGDRSVVCWGSNKSGALGRGKIDEQRHSEASAVT
                     F"
     gene            complement(2950..>4399)
                     /locus_tag="VFV99_07520"
     CDS             complement(2950..>4399)
                     /locus_tag="VFV99_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5059192.1"
                     /translation="AIIFVFGREQCFEIARLLKSCRRFTTDEEKAKIDQMCDEALLPS
                     GASKELRPLLGHGIGIHHAGILPRYKQLVEQLALERLIKFVVSTETIAAGINLPARTV
                     VFPSLRKFIKQQPRMITAAEYHQMAGRAGRPQFDDRGLAITLAPEQVVSDLKKELKNQ
                     KDVERARKTVYGRARNDAQRKGDVIWTPELHAELVKGEPAELRSKTKITAEQVLAIGL
                     PDLAEKALPGSEAEQRMAEAEASLPPSMRLDVVTVIDHLLLTDRERKELQKVLAQLVA
                     NMRAIGVLDEHGKQVSGEMIRSLQGMDGLFIYYVLFNHQLDYVELRQLVEYLIDHDII
                     QRQLDRKGEDEKKEWMRNWLREARIENPHVTWDDAEAKYLQEHPRPLTKIEVIHGEFS
                     GKVPHPELHGGKRPKDVWAQLEDGGFSFLEFVEKHHLEHEEGNLFSYLIRVMNFAQKL
                     YEASQLSELEDMADRIKKLLGRIDMRFVEGLR"
BASE COUNT          664 a         1499 c         1397 g          839 t
ORIGIN      
        1 gaggacgtac tcgcgcgccg cgtaccgctg ctactcgtgt cgcgcgatca aggactcggc
       61 gtatccgagc gcgtcgcgca aaggctcgcc gcgatcctcg gctgggatgc ggctctactc
      121 ggccagatgc tcgacgaata caaggcagag gtcgagctga gccggcgctg gcggagcgag
      181 cgagcttgag gctgtgacag gcctcctcgc gagctacggc atctacggcg ggtcgttgat
      241 cgtcgcgttc atcgcgggct tgttcccgct gttgagcatc gaggtgttcc tcgtgctcgt
      301 atcatcggtg gcaaagccga cgctcacgga gctcacgatg tgctgcgcgc tcgcggcgct
      361 cagccatcag gtcgcgaaga cggcgaccta ctatgccggc gtcggcgcgc tcgagcacgg
      421 caagctcaag gagaagatcg aggccaatcg cgagcgcatc gaacgctgga acaaggcgcc
      481 gctcctggtt ctcgcgctcg gcggtgcctt cgggttcccg ccgctctggg tgctcggctt
      541 catcgcgcgg ccgctcatgc ggatcaacat cgcggcgttc accgtgatcg tgctcgtcac
      601 gcggttcggc cggttcttcg tgctcgcggt gatcccgatg ctgttctagc gcccactaac
      661 gatcgttcgg ccgcatatga tgcggcgcga tcgcaggcag ctcgaagtag aaccgcgcgc
      721 cttggccggg ctggctgatg acgccgatgc ggcccttgtg cgcctcgatg atcttgcggc
      781 tcgtgtagag cccgagtccg tagccttcgg actgcgttgc cgcgcgcttc gtgcggcgat
      841 agcgatcgaa cacgacgcgc gcttcgtccg cgttgagccc ggggcccgag tcgatcaccg
      901 acacgcacgc attcgcgccg cgcgcgtcga gccgcaccgt gatcccgccg tcatccccgc
      961 cgtacttgag tgcgttgtgg acgagattcg agaccacgcg ctcgagccgg ttctcgtcgc
     1021 cgtacacgaa cagcacgtcg cggatctcga gccgcacgtg atcgcgatct tgcgtcggca
     1081 ccgaacgtcc gaccgcgtcg cgcaagaggc gcgacagatc gatgcgatcg aggcgcagct
     1141 cgagccggcc ggagtccgcc gcagacatgt cgagcagatc ggagagcaag cgatcgatgt
     1201 atgacgcgtt ctgcgcgatg cgctcgagac cgcgcgccac cgccggcgtc agccgaagcg
     1261 cgagccgctg ctcgatctga tttgcttcga gcatgatgat cgtcagcggg ttcttcagat
     1321 cgtgcgcgag cgccgctaca tcctcgttcg gtacggaggg cgcggcagca cggcgcgccg
     1381 cttcgcgggc tttctccgcg agctcgtcac gcgtacccac tgcaagcacc ggagtccgct
     1441 gatcgtcttc gaacggatcg acgatttcga catcgcgatc gtccgatgac ggaggctggg
     1501 tgaccctcga catctgactc attgttacgc cgatcggcgc ggatggaaac cccatgcgat
     1561 cgatcggggt tacatgcgcg tacgcatcgg cacaccgcta aaacgtaacg gcgctcgctt
     1621 ctgaatgtct ttgctcgtcg atcttaccgc gccctaacgc acccgattta tttgagcccc
     1681 aacaaaccac ggagcgatct ccgagaaccg cgcatgcgtg agatgggctc gccgcgatcg
     1741 acagcgccgc gccgacgcct gcaatcgccg tcacgagccg gctcttcgta tcgacggccc
     1801 agcaccgcgc gctgccgtcg gtgatgcgcg cgcaattacc agtcacgtaa tccacgctcg
     1861 cgagcccggg ccacggcgtg tcgaccgtgc agccatcttt cggtgcgagg cattcgagct
     1921 tgccggtctt cgtcaccacg cacaggccga catcgaacgc gagatgcttc gcggccggca
     1981 gccgcagcac ttcgatcggc atccccttcg cggcggtctc ctcgggcttc ggcgcggccg
     2041 gcttcgccgc gggtttcgcg gccggcttgc ccttcggcgt cttcttcgca gtctgcttcg
     2101 ctgcgggctt cttcgcgggc gctttcttgg gcgctggttt cggagcagcc ttcggagcgg
     2161 gtttcggcgt cttcggtgcc gccgctgcac accgcgtggc gggccctgcg cacgcgaccg
     2221 aaccgtcgcg acggagccca cacacttcat caccgctcga cgcgatctcg atgacgttcg
     2281 tcatcgcggt cttttgcgga cggccactcg catcccagaa ccagaccgtg ccgtcttcgg
     2341 cgagcgcacc gttcgcggtc accgccgcga tgtgatcgag accgttcgac ggcgtcggca
     2401 cgcgattcat gctgccgccc gacagcttga ggtggcccgc cgcattgata tcgccccagc
     2461 acacgagcga gtgatcctcg agcgtcgcgc acgaggcgct cgcaaccgcg aacagccgct
     2521 tcgccttcgc gacgccgggc accgcggtcg ggctcagtag gttttccttg cggccgaaat
     2581 agatccggcc ccagcaggtc acgctctcgt cgtcgagcag cgcgcacacg tgcgacgcgc
     2641 ccatcacgat gtccttcacg gcgtgcagct tcacggtggt gggcgtcgct gaatcattcg
     2701 tcgtgccgtt gccgagctgg ccttcgttgt tgcgccccca gcagctcacc gtcgcatcgg
     2761 ccatgagcgc gcagctcgtg tggtcgccga ccacgacctt ctgcgctttg gtctcgggcg
     2821 gcggcgcgcc cgcatcgggc tccgggggcg catcgatcgg cttggccggc gcgtcatcct
     2881 tcggcggcgg cttgtacggc acttcctttg gcttgcatgc gacgaggagc gcgaccagga
     2941 ccaacagcct catcgcaggc cctccacgaa gcgcatatcg atccgaccga gcaacttctt
     3001 gatgcgatct gccatgtcct cgagctcgga gagctgcgac gcctcgtaga gcttctgcgc
     3061 gaagttcatg acgcggatca ggtacgagaa caagtttcct tcttcgtgct cgaggtggtg
     3121 cttctcgacg aactcgagga acgagaatcc cccatcctcg agctgcgccc acacgtcctt
     3181 gggccgcttg ccgccgtgca gctcgggatg cggcaccttg cccgagaact cgccgtggat
     3241 gacctcgatc ttcgtcagcg gccgcggatg ttcctggagg tacttcgcct cggcatcgtc
     3301 ccacgtcacg tgcgggttct cgatgcgcgc ctcgcggagc cagttgcgca tccactcctt
     3361 cttctcgtcc tcccccttac gatcgagctg gcgctgaatg atgtcgtgat cgatcaagta
     3421 ctcgacgagc tggcgcagct ccacgtagtc gagctggtgg ttgaacagca cgtaatagat
     3481 gaacaggccg tccatacctt gcaggctccg gatcatctcg ccggagacct gtttgccgtg
     3541 ctcgtcgagc acgccgatcg cacgcatgtt cgcgacgagc tgcgcgagga ctttctgcag
     3601 ctccttgcgc tcgcgatccg tgagcagcaa gtgatcgatg acggtcacga cgtcgagccg
     3661 catcgacggc ggcagtgacg cctccgcctc ggccatgcgc tgctcggctt cgcttcccgg
     3721 cagcgccttc tccgccagat cgggcagccc gatcgcgagc acctgctccg ccgtgatctt
     3781 cgtcttgctg cgcagctcgg cgggctctcc cttgaccagc tcggcgtgca gctccggggt
     3841 ccagatcacg tcgcctttgc gctgcgcatc gttgcgcgcg cggccgtaga ccgtcttgcg
     3901 cgcgcgctcg acgtccttct ggttcttgag ctccttcttg aggtcgctga ccacctgctc
     3961 cggcgcgagc gtgatcgcga ggccgcgatc gtcgaactgc ggacgcccag cgcgacccgc
     4021 catctggtgg tactcggccg cggtgatcat gcgcggttgc tgcttgatga acttgcgcag
     4081 cgacgggaac acgaccgtgc gcgccggcag gttgatgccc gcggcgatgg tctcggtcga
     4141 gacgacgaac ttgatcaggc gctcgagcgc gagctgttcg accagctgct tgtagcgcgg
     4201 caggatgccc gcgtgatgga tgccgatgcc gtggccgagc aacgggcgca gctccttcga
     4261 tgcgccgctc ggcagcagcg cctcgtcgca catctgatcg atcttcgcct tctcctcgtc
     4321 ggtcgtgaac cggcggcatg acttgaggag gcgcgcgatc tcgaagcact gctcgcggcc
     4381 gaacacgaag atgatcgcc
//
LOCUS       DASUFX010000190         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1835485, whole genome shotgun sequence.
ACCESSION   DASUFX010000190 DASUFX010000000
VERSION     DASUFX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835485"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..781)
                     /locus_tag="VFV99_07525"
     CDS             complement(98..781)
                     /locus_tag="VFV99_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059193.1"
                     /translation="MIEEPRGIAREWPNARQRMIAELAVTVTEAPWAMSRQTLGRAHR
                     AGLSDEDVLHVITLASYFGHLNRIADAVQAPLDYNVVLQVPHADPNVAPWPTAPELVV
                     GRPAIDIARRPATANAFTEWRHYIFYSHDAPLTRRQRTLIARWVAAWLGDGGISSPTD
                     LTANPVDEALRTVAETVTLAPWKLTDESFAPLREKGYDDAGIFDVCATATSAGVFSRI
                     EVALVALGT"
     gene            complement(842..1108)
                     /locus_tag="VFV99_07530"
     CDS             complement(842..1108)
                     /locus_tag="VFV99_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059194.1"
                     /translation="MGLAYTNYEGATGPLRELYDKMRERPLPPVYRPAHGGVPGIIAM
                     HSLDPQLIPRVFGTSSTLNGAGPLTWPERELVNAITSRLNQCLY"
     gene            complement(1135..1347)
                     /locus_tag="VFV99_07535"
     CDS             complement(1135..1347)
                     /locus_tag="VFV99_07535"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HEX5059195.1"
                     /translation="MKLKGTITRTDVEGGHWLLKTDDGDQYQLTGKVDGAKDGQRVEV
                     EGKVDKNAMGFGMMGAHFSVQKLTAL"
     gene            1437..2096
                     /locus_tag="VFV99_07540"
     CDS             1437..2096
                     /locus_tag="VFV99_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059196.1"
                     /translation="MRWLPLLLVLVCASAAANPVRRDLDIFERDVRFRKGKRPYKPSP
                     PPCKQTSSWRKYTKCQLKGLEIEMIQDQPTMKLVVYKTPYNPNSKVVALHFLTGNAWV
                     QSGFYTELNTTTELLGYRKAGERFRLDVGYATPTWVTLDEVSTRPATLRRQFTYFCTA
                     TGGCPSVMTACDVIVHGKAVSSFRGEPVWEGNMLKVRGNASATNRYCTKPPNLIDVSD
                     E"
     gene            2093..2707
                     /locus_tag="VFV99_07545"
     CDS             2093..2707
                     /locus_tag="VFV99_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059197.1"
                     /translation="MKRWLVLALLLASTGTASPRRVVHMPTLPEVCPSNAEWDKLDAC
                     IRRHGTMKVLRDEPALKIIDLDDRMSRFGGIYVYAFKTKWDMRGTMRLWQEHDVLGVQ
                     HVTYGKHKGIRFDVGLSTAMPFTLDGETSMQGVLRQQETMVCFEDAWGCLQAITSCEL
                     LMRGKAYYSFRGTLAYADKQLQIVGDRSRSGTYCTSPEIMLQDQ"
     gene            complement(2701..>3228)
                     /locus_tag="VFV99_07550"
     CDS             complement(2701..>3228)
                     /locus_tag="VFV99_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5059198.1"
                     /translation="NAYPRAYHELIEKHEKLGKNPEGYLHSIMRKESGFDPHDLSYAD
                     AQGLLQMIPATTMRVAKALKLPYDPGKLYEPEYNIMTGSWYIGHMLEKFKGQIPLGAG
                     SFNSGPRPVMRWLDQYGDREIDELVELVPYTQTREYMKKVTENFARYRYLYKGEIYDQ
                     PLTVDKHYVVDQLTY"
BASE COUNT          563 a         1020 c         1046 g          599 t
ORIGIN      
        1 gatcgttcaa tagcgctgca cgtcgtcgct gaggatcatc atgccaccaa gccaatactc
       61 gagctgctca ccgcataggt gccgctcgag tgcgcggcta ggttccgagc gcgacgagcg
      121 cgacttcgat gcgcgagaac acgcctgccg acgtcgcggt cgcacacacg tcgaagatgc
      181 ccgcgtcgtc gtagcccttc tcgcgaagcg gtgcgaacga ttcgtcggtg agcttccacg
      241 gtgcgagcgt caccgtctcg gcgaccgtgc gcagcgcttc gtcgacggga ttggcggtta
      301 gatccgtggg tgacgagatg ccgccgtcac cgagccacgc cgcgacccag cgtgcgatca
      361 gtgtgcgttg ccggcgcgtg agcggcgcgt cgtgcgagta gaagatgtag tgacgccact
      421 cggtgaacgc gttcgctgtt gccggtcgac gcgcgatgtc gatcgccggt ctgcccacga
      481 caagctcggg tgccgtcggc cacggcgcga cgttcggatc cgcgtgcggt acttggagca
      541 ccacgttgta gtcgagtggc gcctgcaccg catcggcgat gcgattgaga tgaccgaagt
      601 acgacgcgag cgtgatcacg tgcagcacgt cttcgtcgct gaggccggcg cggtgtgcgc
      661 gaccgagcgt ctgccgcgac atcgcccatg gcgcctcggt gaccgtgacc gcgagctcgg
      721 cgatcatgcg ctgccgcgcg ttcggccact cgcgcgcgat gccgcgcggc tcctcgatca
      781 cgtgccgtgc gaacgtttca tcttcggacg cgacacgcag aaactctgcg tgacaggccg
      841 ttcaatacaa acactggttc agtcgtgatg tgatcgcgtt gacgagctcg cgctccggcc
      901 acgtcagcgg accggcgccg ttgagcgtcg atgacgtgcc gaacacgcgt gggatcagct
      961 gcggatcgag gctatgcatc gcgatgatgc cgggcacgcc accgtgcgcc ggccgataga
     1021 ccggcggcag cggccgctcg cgcatcttgt cgtagagctc gcgtagcgga ccggtcgctc
     1081 cctcgtagtt cgtgtacgcg agacccacgg aacaatcgta tcaaaccgcg cgaatcagag
     1141 cgcggtgagc ttttgcacgc tgaagtgggc gcccatcatc ccgaagccca tcgcgttctt
     1201 gtcgaccttt ccctccacct cgacgcgctg gccgtccttc gcaccgtcga ccttgccggt
     1261 cagctgatac tgatcgccgt cgtcggtctt gagcagccaa tggccgcctt cgacgtccgt
     1321 ccgggtgatc gtgcccttga gcttcatacc tgcacgatag ctggtgattt gagttgcggc
     1381 caccgggtcg aaaaccccag cacgatcggc cgtctcgtgc gttcataatc agcctgatgc
     1441 gctggctgcc gcttctcttg gtgctcgtgt gcgcgagcgc ggccgccaac ccggttcggc
     1501 gcgacctcga catcttcgag agagacgtcc gcttccggaa gggcaagcgg ccctacaaac
     1561 cgtctccgcc gccgtgcaag cagacctcga gctggcggaa gtacacgaag tgtcagctca
     1621 agggcctcga gatcgagatg atccaggacc agccgacgat gaagctcgtc gtctacaaga
     1681 cgccgtacaa cccgaattcg aaagtcgtcg cgctgcactt cttgacgggt aacgcgtggg
     1741 ttcagtcggg cttctacacc gagctcaaca cgaccaccga gctgctcggc taccgcaagg
     1801 ccggtgagcg gtttcgactc gacgtcggct acgcgacgcc aacctgggtg acgctcgacg
     1861 aagtgagcac gcgcccggcg acactgcgcc ggcagttcac gtacttctgc accgcaaccg
     1921 gcggatgccc gtcggtgatg accgcgtgcg atgtgatcgt gcacggcaag gcggtgtcgt
     1981 cgtttcgcgg cgagccggtg tgggaaggca acatgctcaa ggtgcgtggc aacgcgagcg
     2041 cgacgaaccg ctactgcacg aagccaccga acctgatcga cgtgagcgac gaatgaagcg
     2101 ctggctcgtt ctcgcgctct tgctcgcgtc gacgggcacc gcgtcaccgc ggcgggtcgt
     2161 gcacatgccg acgctgcccg aggtctgccc gagcaacgca gagtgggaca agctcgacgc
     2221 gtgcattcgc cgtcacggca cgatgaaggt gctgcgtgac gagccggcgc tgaagatcat
     2281 cgacctcgac gaccgcatgt ctcggttcgg cgggatctac gtctacgcat tcaagaccaa
     2341 gtgggacatg cgcggaacga tgcggctgtg gcaagagcac gacgtgctcg gcgtccagca
     2401 cgtcacgtac ggcaagcaca aaggcattcg cttcgacgtc gggctgtcga cggcgatgcc
     2461 cttcacgctc gacggcgaaa cgagcatgca aggcgtgctg cgccagcagg agacgatggt
     2521 gtgcttcgag gacgcgtggg gctgcctgca ggcgatcacg agctgcgagc tactcatgcg
     2581 cggcaaggcg tactactcgt ttcgcggcac gctggcgtac gcagacaagc agttgcagat
     2641 cgtcggcgat cgctctcgct cgggaacgta ctgcacgtcg cccgagatca tgttgcagga
     2701 tcagtaggtg agctgatcga cgacgtagtg cttgtcgacg gtgagcggct ggtcgtagat
     2761 ctcgcccttg tagaggtagc gatagcgcgc gaagttctcg gtgaccttct tcatgtactc
     2821 gcgcgtctgc gtgtacggca ctagctcgac gagctcatcg atctcgcgat cgccgtattg
     2881 atcgagccag cgcatcaccg ggcgcggtcc gctgttgaac gagccggcac cgagcgggat
     2941 ctgccctttg aacttctcga gcatgtgccc gatgtaccag ctgccagtca tgatgttgta
     3001 ctcgggctcg tagagcttgc ccgggtcgta gggaagcttg agcgccttcg cgacgcgcat
     3061 cgtcgtcgcc gggatcatct gcagcaggcc ctgcgcatcg gcgtacgaga gatcgtgcgg
     3121 atcgaaaccg ctctccttgc gcatgatcga gtgcaggtac ccttcgggat tcttgccaag
     3181 cttctcgtgc ttctcgatca gctcgtggta cgcgcgcgga tacgcgtt
//
LOCUS       DASUFX010000191         7663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1696311, whole genome shotgun sequence.
ACCESSION   DASUFX010000191 DASUFX010000000
VERSION     DASUFX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696311"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFV99_07555"
     CDS             complement(<1..616)
                     /locus_tag="VFV99_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5059199.1"
                     /translation="MKFRNVGLVALVVTAIVGCDRGNHDGARVTQSGSGGGAAADPWN
                     TPAPAKDPLKKPLLWKVEKDGNTSYLLGTIHVGVDPTSRLPDVVWQKLDEKPIFAMET
                     DLSDASKLEVKRSDGTTLEDEIGPEYWKKLEDALGAKEASAMLPMKPMIPATLLSLRG
                     LPKTAPMDGVLHGRASNAHKQIVYLEALEVQSKMLDKWMNARALK"
     gene            639..1106
                     /locus_tag="VFV99_07560"
     CDS             639..1106
                     /locus_tag="VFV99_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059200.1"
                     /translation="MGRLGALLIVATMVMSSTARADEDIQLALSDRWREVPPSQRLKL
                     SDQITEQLTELGNFIGTHVNVLSDDILMMKFDGRRRYARIRFGTGEDAYLRFKFDSDW
                     YFQQGKARVQARLDLGVGTHQFHLELPDVDMVPASYRGDRGVELRLPLFERRW"
     gene            complement(1161..2381)
                     /locus_tag="VFV99_07565"
     CDS             complement(1161..2381)
                     /locus_tag="VFV99_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059201.1"
                     /translation="MPPAPPVAAAGPREAPPPPRQEKIGRRNGFVWVSGNWDWKNGAW
                     AWEPGHWEKVRKGKKWRARRWEQKGDVWVRIDGDWDDDRPNAAPPALQEEKWQPRRGW
                     VFVRGNWDWQNGAWAWTPGHWERERANKRWREPKWELQGSVWVMVNGDWIDAPKFPDA
                     APPAPRDEKFGVRAGFVWVKGRWDWKNGNWDWVAGHWERARANYTWTEGSWQNKNGQW
                     VWVDGAWTAAAPLPQFPTAAPPAPQAEQIAPKAGHVWVKGRWDWKAGKWAWTPGHWER
                     VRANYTWQDGSWQNQGGKWVWVEGQWVAAAPPAPAPTPPPPPAPPVVQAPPTPPPGPT
                     SAPPAPQAENVQPRAGFVWVRGHWGWSGNSKYEWIAGHWERQRANVQWYDGKWEQRTQ
                     GNVTYWVWIEGGWR"
     gene            2615..3223
                     /locus_tag="VFV99_07570"
     CDS             2615..3223
                     /locus_tag="VFV99_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059202.1"
                     /translation="MILRIALVGLLTLVACGDRKSAEPTRRSPLEVGIARDLTAKLGE
                     PVTASCVMIQNLPAKCEAALADGTKLPIVITSEGKEWAWRVGGRVVESAPIVAYINAA
                     LADLKIAQQASCGPRIVFVEPGGRIGCKLSGGGMGFVRVAVDGTTSLELDIDPASAAA
                     RGEVVTPQRDRELTTISKALENLEGESDGEEEVPGDGGVPSP"
     gene            3237..4994
                     /locus_tag="VFV99_07575"
     CDS             3237..4994
                     /locus_tag="VFV99_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5059203.1"
                     /translation="MRSVWVVASCAFALSCGQPTTKQHVEHPPAQTQKGSRTITVIGT
                     NDLHGAIDRLPLFGGFLSNVRAAREADGGAVLLLDAGDMFQGTLESNLAEGADVVKAY
                     NQLGYTAVAVGNHEFDFGPEGPAAVAKSIEDDPRGALKQRVAEAKFPFLVANIDDAKT
                     GERIKWKNMPPSMMVESAGTMIGIIGAATESTPYTTMPANFLGLKMAPTAAAITKEAR
                     ALRAKGAQIIIVVAHIGSACKDTTNPNDISSCNKDEEVFKVISDLPKGLVDVWIGGHT
                     HAAIAQRISDVAVIESFSSGRAFGRVDLRVTGTHVSGVTIYQPTLLCDLDKDGNPEPV
                     AQCKPDPYEGHPVTADAEVQKLVDAAVVHAGKLRDEKLGIALTGIMTKSYSLESAEGN
                     FFTDLMLAARPEAQVALTNGGGLRADIPAGDLTYGRLYEAMPFDNRFALIDLKGKHIR
                     RLITTNIQRGSGIYSWGGVSAKARCKAGLLDVEIKIGGKPLADDATYKLVTSDFLASG
                     GDISAIGRLKLPEGSIQMTDVIIRDAIADVLRKEAKKKGKVDPLQYFSAKNKRLDYEG
                     ERPVLCQGAKPTRTSEEPD"
     gene            4994..6346
                     /locus_tag="VFV99_07580"
     CDS             4994..6346
                     /locus_tag="VFV99_07580"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX5059204.1"
                     /translation="MVMKETPDWSPESWRARPAAQQVGYGDTQTLARVMAEMAALPPL
                     VTSWEIEDLRNQLGKAARGEAFLLQGGDCAESFDDCRSEPIAAKLKILLQMSLVLVHG
                     TRKPVIRVGRIAGQYAKPRSADNETRGDVTLPAYRGDLINRDAFTAEDREPDPTLMLR
                     AYERAALTLNFIRALTEGGFADLHHPEYWDVSFAQGSPNAAHYHRIVESIRESLDFVA
                     AITGVESDVLRRVHVFTSHEALALPYEQAQTRRVPRRAGYYNLSTHFPWIGMRTAQLD
                     GAHVEFHRGIRNPLGIKIGPAMTIDWLQGLLEVLDPNNEPGRITLIHRMGAAKVQDKL
                     PALIDAVRSAGRRVLWVCDPMHGNTETTPQGIKTRRFDNIISELEQSFELHHKLGSHL
                     GGVHIELTGENVTECVGGARGLTEADLAHAYKSRVDPRLNYEQALETALRISRHLRGS
                     "
     gene            6493..7122
                     /locus_tag="VFV99_07585"
     CDS             6493..7122
                     /locus_tag="VFV99_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059205.1"
                     /translation="MSWVLPLVGMGLAYTVLAWSSDTDNTGKAWMAVGFGFVVVIWSV
                     FRILVDQTALARAVAANDVPRILAITDKQLARKRGDPKRGPFLVYRAYAQEAQRDYTS
                     ALATLDEAKPADPGLQLLAAALRVLALVETGDLASARRVETDELEPRAAKLDARLHAA
                     PHIHANLARGRLLLAEGKTDEARAQLRRVVDDIRAGSPLRVRARELMAG"
     gene            complement(7132..>7663)
                     /locus_tag="VFV99_07590"
     CDS             complement(7132..>7663)
                     /locus_tag="VFV99_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059206.1"
                     /translation="AEVDPGHHTLVAGAPGRKIFTQEFEVEGRGGQTIDVALVEGANT
                     VIVDRGAGRRKAAIILAGGGVVLWGASAGIAFWAKGKYDKYAKDGMAITGQTDGGSYC
                     SPTGCTLDESTKAANHYQTIARWVATPIFIGGAIAIGGAIVMYVTAPDKERIDQTVFA
                     PIVTPDQVGFAITRGF"
BASE COUNT         1312 a         2621 c         2437 g         1293 t
ORIGIN      
        1 ccttgagtgc gcgggcgttc atccacttgt cgagcatctt cgactgcacc tccagcgcct
       61 ccagatagac gatctgcttg tgcgcgttcg acgcgcggcc gtgcagcacg ccgtccatcg
      121 gcgcggtctt cggcagtccc ctcagcgaga ggagcgtcgc cgggatcatc ggcttcatcg
      181 gcagcatcgc gctggcctct ttcgcgccga gcgcatcctc gagcttcttc cagtactcgg
      241 gaccgatctc gtcctcgagc gtcgtgccgt cgctgcgctt cacctcgagc ttgctcgcgt
      301 ccgacaggtc cgtctccatc gcgaagatcg gtttctcgtc gagcttctgc cagacgacgt
      361 cgggcaggcg cgacgtcgga tcgacgccga cgtggatcgt gccgagcaga tagctcgtgt
      421 tgccatcctt ctcgaccttc cagagcagcg gttttttcag cggatctttt gccggtgccg
      481 gtgtgttcca ggggtcggcc gccgcgccgc cgccactgcc actttgtgtg acacgcgccc
      541 cgtcgtgatt gccgcgatcg cacccgacga tggcggttac gacgagcgcc accagaccga
      601 cattgcgaaa cttcatgacc gtgctttagc attaaaccgt ggggcgactc ggggctttgc
      661 tgatcgtggc gacgatggtg atgtcgtcga cggcgcgcgc cgacgaggac atccagctcg
      721 cgctgtccga tcgctggcgc gaggttccgc cgagccagcg cctcaagctg tcggatcaga
      781 tcaccgagca gctcaccgag ctcggtaact tcatcggcac gcacgtcaac gtgctgtcgg
      841 atgacatcct gatgatgaag ttcgacggcc gccgccgcta tgcgcgtatt cgcttcggca
      901 cgggcgagga cgcgtacctg cgcttcaagt tcgatagcga ctggtacttc cagcagggca
      961 aggcacgcgt gcaagcgcgg ctcgatctcg gcgtcggcac gcatcagttc catctcgagc
     1021 tgcccgacgt cgacatggtg cccgcgagct atcgcggcga tcgcggcgtc gagcttcgct
     1081 tgccgctgtt cgaacgccgc tggtgaaaaa aaagagccgc gacgttctcg cggctctctt
     1141 cgttcacgaa cgagtgacga ctaccgccag ccgccctcga tccacaccca gtacgtcacg
     1201 ttgccctgcg tgcgctgctc ccacttgccg tcgtaccact ggacgttcgc gcgctggcgc
     1261 tcccagtgac ccgcgatcca ttcgtacttg ctgttgcccg accagcccca gtgaccgcga
     1321 acccagacga agccagcacg cggctggacg ttctcggcct gcggtgcggg cggagcactc
     1381 gtcggacccg gcggtggcgt cggcggagct tgcaccaccg gcggagcagg cggcggggga
     1441 ggcgtcggcg cgggagccgg cggcgctgcc gcgacccact gaccttcaac ccaaacccac
     1501 ttgccacctt ggttctgcca gctgccgtct tgccacgtgt agttcgcgcg cacgcgttcc
     1561 cagtgacccg gcgtccacgc ccacttgcca gccttccaat cccagcgacc cttcacccag
     1621 acatggccgg ccttcggagc gatctgctcg gcttgcggcg cgggcggtgc ggccgtcggg
     1681 aactgcggca acggagctgc cgcggtccac gcgccgtcga cccacaccca ctggccgttc
     1741 ttgttctgcc agctgccttc ggtccaggtg tagttcgcgc gcgcgcgctc ccagtgaccc
     1801 gcgacccaat cccagttgcc gttcttccaa tcccagcgac ccttcaccca gacgaagccg
     1861 gcgcgcacgc cgaacttctc gtcacgcggt gcgggcggcg cggcatcggg gaacttcggc
     1921 gcatcgatcc aatcgccgtt gaccatcacc cagacgctgc cttgcagctc ccacttcggc
     1981 tcgcgccagc gcttgttcgc gcgctcgcgc tcccagtgac caggcgtcca cgcccatgca
     2041 ccgttttgcc agtcccagtt gccgcgaacg aacacccaac cgcgacgcgg ttgccacttc
     2101 tcttcttgca gtgcgggcgg cgcagcgttc ggacgatcgt cgtcccaatc accgtcgatc
     2161 ctcacccaga catcgccctt ctgttcccag cgacgggcgc gccacttctt gcccttgcgg
     2221 actttctccc aatgaccggg ttcccacgcc cacgcaccgt tcttccaatc ccagttgccg
     2281 ctcacccaca cgaagccgtt gcggcggccg atcttctcct gcctcggtgg cggaggagct
     2341 tcacgtggac ctgcagcggc gaccggcgga gccggcggca tcacggcgga tggtggtgga
     2401 cccttggggc ccttgcgatg atcgcgaacc gctggttgcg caccggcaac cgaaaccgtg
     2461 agcagcgtgc tacctgcaat cgtcagaagc caacgtgaaa tattcgatcg catcgttcct
     2521 cctaaaccga acgtgggacg gaacttatcg cggattattc acgcgaacca tgtcgggcga
     2581 ccctcgaagt tcttgtcgtg ttaccaacac ataggtgatt ctccgaatcg cgcttgtagg
     2641 tctgctgacc ctcgtcgcgt gtggcgaccg caagagtgca gaacccacac ggcgctcgcc
     2701 gctcgaagtc ggcatcgcgc gcgatctgac cgcgaagctc ggcgaaccgg tgaccgcgag
     2761 ctgcgtgatg atccagaacc tgcccgcgaa gtgcgaggca gcactcgcgg atggtacgaa
     2821 gctgccgatc gtcatcacga gcgaaggcaa ggaatgggcg tggcgcgttg gtggccgcgt
     2881 cgtcgagtcc gcgccgatcg ttgcatatat caacgccgcg ctcgccgacc tgaagatcgc
     2941 gcagcaggcg agctgcggac cgcgcatcgt gtttgtcgag ccgggcggcc gcatcggctg
     3001 caagctgtca ggcggcggaa tggggttcgt gcgcgttgcc gtcgacggca cgacgtcgct
     3061 ggagctcgac atcgatcccg caagtgctgc agcccgcggc gaagtagtga ctccgcaacg
     3121 tgatcgcgag ctgacgacga tttcgaaagc actcgaaaat ctcgaaggcg aatcggacgg
     3181 cgaggaagaa gtaccgggcg atggcggcgt gccaagtcca tagtagagtc ggcgctatga
     3241 gatccgtctg ggtggtcgca tcatgcgcat tcgcgctgtc gtgcggacaa cccacgacga
     3301 agcagcacgt cgagcatccg ccggcgcaaa cccagaaagg ttcgcgcacc atcacggtga
     3361 tcggtacgaa cgatctgcac ggcgcgatcg atcggctgcc gctgttcggt gggttcctgt
     3421 cgaacgtccg cgcggcacgc gaagcggacg gcggcgcggt gctcctgctc gacgcgggcg
     3481 acatgtttca aggcacgctc gagtcgaacc tcgccgaggg tgccgacgtc gtgaaggcgt
     3541 acaaccagct cggttacacc gcagtcgccg tcggcaatca cgagttcgac ttcgggccgg
     3601 aaggtccggc cgcggtcgcg aagtcgatcg aggacgatcc gcgcggcgcg ctcaagcagc
     3661 gcgttgcaga agcgaagttc ccgttcctcg ttgccaacat cgacgacgcg aagaccggcg
     3721 agcgcatcaa gtggaagaac atgccgccgt cgatgatggt cgagtcggca ggcacgatga
     3781 tcggcatcat cggcgcggcc accgaatcga cgccgtacac gacgatgccg gcgaacttcc
     3841 tcggcctcaa gatggcgccg accgcagcgg cgatcacgaa ggaggcgagg gcgctacgcg
     3901 cgaagggcgc gcagatcatc atcgtcgtcg cgcatatcgg cagcgcgtgc aaagacacga
     3961 caaacccgaa cgacatctcg tcgtgcaaca aggacgaaga ggtgttcaag gtcatcagcg
     4021 atctgccgaa ggggctcgtc gacgtctgga tcggcggtca cacccacgcg gccatcgcgc
     4081 agcgcatctc cgatgtcgcc gtgatcgagt cgttctcgtc ggggcgtgcg ttcggtcgcg
     4141 tcgatctacg cgtcaccggc acgcatgtca gcggcgtcac catctatcag ccgacgctgc
     4201 tctgcgatct cgataaggac ggcaaccccg agccggtcgc gcagtgcaag cccgacccgt
     4261 acgaaggcca cccggtcacc gccgacgccg aggtgcagaa gctcgtcgac gcggccgtcg
     4321 tgcacgcggg caagctgcgc gacgagaagc tcggcatcgc gctgacgggc atcatgacga
     4381 agagctactc gctcgagtcc gccgaaggga acttcttcac cgatctgatg ctcgccgcac
     4441 gtcccgaggc acaggtcgcg ctgacgaacg gcggcggcct gcgcgcagac attccggccg
     4501 gcgatctcac gtatggccgg ctctacgaag cgatgccatt cgacaaccgc ttcgcgctga
     4561 tcgatctcaa gggcaagcac atccgccgtc tgatcacgac gaacatccag cgcggcagcg
     4621 gcatttactc gtggggcggc gtcagcgcga aggcgcgttg caaggcgggc ctgctcgacg
     4681 tcgagatcaa gatcggcggc aagccgctcg ccgacgatgc gacctacaag ctcgtcacca
     4741 gcgatttcct cgcgtcgggc ggcgacatca gcgcgatcgg ccggctcaag ctgcccgagg
     4801 gctcgatcca gatgaccgat gtcatcattc gcgacgcgat cgcggacgtg ctgcgcaagg
     4861 aagccaagaa gaagggcaag gtcgatcctt tgcagtactt ctcggcgaag aacaaacggc
     4921 tcgattacga aggcgagcgc ccggtcctgt gccagggcgc gaagccaacg cggacatccg
     4981 aggagccgga ctgatggtga tgaaggaaac cccggactgg tcgcccgaga gctggcgtgc
     5041 gcgccctgct gcgcagcaag tcggctacgg cgacacccaa acactcgcgc gtgtgatggc
     5101 ggagatggca gcgttgccac cgctcgtcac gtcgtgggaa atcgaagacc tccgcaacca
     5161 gctcggcaag gcagcgcgcg gcgaagcgtt cctgctgcag ggcggcgact gcgccgaaag
     5221 ctttgacgac tgccgctcgg agccgatcgc cgcgaagctc aagatcctgc tccagatgtc
     5281 gctcgtgctg gtgcacggca cgcgcaagcc agtgatccgc gtcggtcgca ttgccgggca
     5341 gtacgcgaag ccccgctcgg ccgataacga gacgcgcggc gatgtcacgc tgcccgcgta
     5401 ccgcggcgac ttgatcaacc gcgatgcgtt caccgccgaa gatcgggagc ccgatccgac
     5461 gctgatgcta cgcgcctacg agcgcgctgc gctcacgctc aatttcattc gcgcgctgac
     5521 cgaaggcggc ttcgccgatc tccaccaccc ggaatactgg gacgtctcgt tcgcgcaagg
     5581 ctcgccgaac gcggcgcact accaccgcat cgtcgagtcg attcgcgagt cgctcgactt
     5641 cgtcgccgcg atcaccggcg tcgagtccga cgtcctgcgc cgcgttcacg tgttcacgag
     5701 ccacgaagcg ctcgcgctgc cgtacgagca ggcgcagacg cgtcgcgtgc cgcgtcgcgc
     5761 gggctactac aacctgtcga cgcacttccc gtggatcggc atgcgcaccg ctcagctcga
     5821 cggcgcgcac gtcgagttcc atcgcggcat tcgcaatccg ctcgggatca agatcggtcc
     5881 ggcgatgacc atcgactggc tgcaaggcct gctcgaggtc ctcgatccga acaacgagcc
     5941 gggccggatc acgctcatcc accgcatggg cgccgccaag gtgcaggaca agttgcctgc
     6001 cctcatcgac gccgtgcgca gcgcgggccg cagggtgctg tgggtctgcg atccgatgca
     6061 cggcaacacc gagacgaccc cgcaaggcat caagacgcgt cgcttcgaca acatcatctc
     6121 cgagctcgaa cagtcgttcg agctgcatca caagctcggt tcgcacctcg gaggtgtgca
     6181 catcgagctc accggtgaga atgtcaccga gtgtgtcggc ggtgcgcgcg gcctcaccga
     6241 ggccgacctc gcgcatgcgt acaagagccg cgtcgatccg cgcctcaact acgaacaagc
     6301 cctcgagacc gcgctgcgga tctcgcgcca tttgcgcggc tcgtagcttc gtcggtgggc
     6361 ctgcatcttg cggcgacatc cgcggcggca gttcgtcgca ccgcagcgtc gtgcgcgccg
     6421 tggtatggca acccgcgacg tatgaccggg caggcaccac agacgcaatc cacgagccgc
     6481 ttgctgtggt tcgtgagctg ggtgctgccg ctcgtcggca tgggcctcgc gtacacggtg
     6541 ctcgcgtggt cgagcgacac cgacaacacc ggcaaggcgt ggatggcggt cgggttcggc
     6601 ttcgtcgtcg tgatctggag cgtgttccgc atcctcgtcg atcagacggc gctcgcgcgc
     6661 gcggtcgccg cgaacgatgt cccgcgcatc ctcgcgatca cggacaagca gctcgcacgc
     6721 aagcgtggcg acccgaagcg cggcccgttt ctcgtctatc gcgcttatgc gcaggaggcg
     6781 cagcgcgact acacgtccgc gctcgccacg ctcgacgagg cgaagcccgc tgacccaggc
     6841 ctccagttgc ttgccgctgc actgcgcgtg ctcgcgctcg tcgagaccgg cgatttagcg
     6901 agcgctcgcc gcgtcgagac cgacgagctc gagccccgcg cggcgaagct cgacgcgcgc
     6961 ctgcacgcgg cgccgcacat tcacgcgaac ctcgcgcgcg gccgcttgct gctcgccgaa
     7021 ggcaagaccg atgaagcgcg cgcgcagctc aggcgcgtcg tcgacgacat tcgcgccggt
     7081 agcccgctgc gcgtccgtgc gcgcgagctg atggcgggct aacgagcggc gctagaagcc
     7141 gcgcgtgatc gcgaagccaa cctggtcggg cgtcacgatc ggtgcgaaca cggtttgatc
     7201 gatgcgctct ttgtcgggcg cggtcacgta catcacgatc gcaccgccga tcgcgatcgc
     7261 gccaccgatg aagatcggcg tcgcaaccca gcgcgcgatc gtctggtagt ggttcgcggc
     7321 cttggtcgat tcgtcgagcg tgcagccggt cggtgagcag tagctgccgc cgtcggtctg
     7381 gccggtgatc gccatgccgt ccttggcgta cttgtcgtat ttgcccttcg cccagaacgc
     7441 gatgcctgcc gacgcgcccc acagcacgac gccgccgcca gcgagaatga tcgctgcttt
     7501 gcgacgaccc gcgccgcgat cgacgatgac ggtgttcgcg ccttcgacaa gcgcgacgtc
     7561 gatcgtttgg ccaccgcggc cctcgacctc gaactcctgc gtgaagatct tgcgacccgg
     7621 cgcgcctgcg acgagtgtgt gatggcccgg atcgacttcg gcg
//
LOCUS       DASUFX010000192         7080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1558969, whole genome shotgun sequence.
ACCESSION   DASUFX010000192 DASUFX010000000
VERSION     DASUFX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558969"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..423)
                     /locus_tag="VFV99_07595"
     CDS             complement(121..423)
                     /locus_tag="VFV99_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059207.1"
                     /translation="MGSFAQGAGYTLEVVTLSKVSVTYVNIRRDKISEVESAVFDGTS
                     GSVPPPVQRTIGSNIGRIVILANALIGENAKVKITSAGNLFEYTVNPDGDLVFDVV"
     gene            479..2431
                     /locus_tag="VFV99_07600"
     CDS             479..2431
                     /locus_tag="VFV99_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5059208.1"
                     /translation="MRTLVGTCVIAIGCGSGSSTPDASRNDSLQLDADIDGNAIDAPD
                     ANTGSGVRVGGTAFGIRAPVEVTLTVDSASHRMIVTEDGAFRFPRKFLSGTYSVTETS
                     ADCVIENGTGTLTGSEVTNVSLICRGVVELGSIAFAPNVVRLATTFDPGAPLTYTATR
                     PFFADATDTITVTPTALYPALASLAINAAPASSGQPSVQPYNAQTPVALAYGSAFANI
                     YTFATTPEVSFEAYVKASDTAMQAGFGSGQSTACMGATNKNGNGVALSGDTLVVGSPC
                     INVRAGAAYVYRRTGTTWAFEAKLTPTVADMNDLFGTSVAIDGDSIVVGAFGEASSNG
                     TATDNAAPNAGAAYVFTRSGTAWSQQAYLKATNIDAADRFGTSVAIAGDTVIVGAPGE
                     SSSGTGINGTANNTASAAGAAYIFTRSGTTWSQQAYVKASNTGANDAFGTAVAIASNT
                     AVVGAPNEDGATGDDGAVYVFVRNGTTWSQQGYLKASNAGASDTFGTSVAVDGNLLIG
                     GAPGEDGNGSLAENDSGAAYVFTRNGTTWTEDAMLFANTRVQSGGFGQAVAIDRTHAV
                     VGSRLAGNGIIQAYRRSATAWDTIRFRNAPNMDVAGLDEFGSSVAADGDTFAAGAFKE
                     DSNATNVGGNGADNSATDSGAVYVFR"
     gene            complement(2721..4448)
                     /locus_tag="VFV99_07605"
     CDS             complement(2721..4448)
                     /locus_tag="VFV99_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059209.1"
                     /translation="MKLSLRYKLIGLFCVLVVVVGAFTLYWIERTLADDLLEALDKRL
                     SDQGTAVSKWLTIAGHVDRLTPRLAEVTGTRLTIVGADGVVQGDSHEPQSVGENIGSA
                     WEVAQARKGQVARAVRQLRPDEPKQYLVAVPAELGRVIRLAVPLGDIVDARARMRNRL
                     IVGFGFGFLGALFLSWIFLRAITKPLQSMTRTAETLAAGNYDVAPPVAATDAGGELSV
                     LARAMMRMAGEVKSRVNELTEQRDLLSVVLGGLVEGVVVVDPDGTIVLANTAARPLIG
                     DSLPAALAKLVDAARVGEQPEQELELVGRTVRASARPLRAPGKTTGEQNQVGLPATPP
                     QLSRPAVIVVLYDVTRMRALEAVRRDFLSNAAHELRTPVTSISGYAETLLGGGIDAEL
                     SKEFITTIHRNARRISDMVSDLLVLDTLGGRAAVVGERVSVPLLEVVDDAVRTAKGVT
                     PSAQIDVDVGAIAVLGTREGLEHVVQNLIDNAIKYGNGAPVSVRATKREGKVRLAIAD
                     AGPGIPAGHEDRIFERFYRVDPGRSRDQGGTGLGLAIVKSHVEAMGGRVWFEHAEPGA
                     RFVIELDAA"
     gene            complement(4448..5149)
                     /locus_tag="VFV99_07610"
     CDS             complement(4448..5149)
                     /locus_tag="VFV99_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059210.1"
                     /translation="MERRVVIIEDERDVARLLEFNLQQAGFTVEACGTGNDGLAAIRK
                     HPPDVVVLDLMLPDTSGYEVCKTIRGESPLADIGVLMLTAKGEAEDRILGLEVGADDY
                     VVKPFVVREVVLRVTALANRLAERRARPSQSTGTSLKVGPIELDPVTHDVRIQGAPTQ
                     LRPLEYKLLQLLVSDPGRVFSREELLEQVWEMRGDINTRTVDVHVRRLRVSLGPAADV
                     IETVHGFGYRARRDA"
     gene            5205..5978
                     /gene="modA"
                     /locus_tag="VFV99_07615"
     CDS             5205..5978
                     /gene="modA"
                     /locus_tag="VFV99_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5059211.1"
                     /translation="MRQLAVGLVVLAAACSSKKEAPANTAPKEVHVAAASDLTQAFTE
                     VGAAFEKKTGIKPNFNFSSSGLLAKQVEQGAPLYLFAAANKSFVDQVVKAGKCDAATA
                     QLYSRGRLVMWAKGGPPAKITDLVDPKYKRIGIANPDHAPYGKAAKQALEKAGIYEQV
                     KDRLVLGENISATMTYAKEGSVDVAFVALSLAISTDAQMYSPVELDLHDPLEQTLVVC
                     GKGPEADAAKQLAEFIISTDGRTIMEHYGFSTTGVMTQK"
     gene            5978..7078
                     /locus_tag="VFV99_07620"
     CDS             5978..7078
                     /locus_tag="VFV99_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX5059212.1"
                     /translation="MRPETIAVKAIEIDEATGALVPPIHLSTTYVRDAEYRLIADRDY
                     TRDKNPTPLGAEHAIAALEGGARALVFSSGMAAATSLVRSLTKPGDHVLAPKVAYYAL
                     RGWFERFTTKWGIGLDFVDMTNLDAVRGALRDTTRLVWLETPANPTWDVTDIAAVAEL
                     AHRKGALVAVDSTCATPVHTRPLTLGADLVMHSATKYLGGHSDVLAGALVTREDNAAW
                     AEIHRTRHDEGAVIGPVEAFLLGRGIRTLFARVERQSRTALSLATRLAERGVTVCYPG
                     LPSHPQHAVAAKQMTGGFGGMLSIRVPDPLALVKKLRVWMPATSLGGVESLIEHRASV
                     EGPTSPTPKDLLRLSVGLEHEDDLFEDLVQAL"
BASE COUNT         1263 a         2360 c         2312 g         1145 t
ORIGIN      
        1 gatcacgaac gactcgctgc tcgtcggtgc accctatgaa gactcgggcg cgacgggctg
       61 gaacggttca caaggcgacg gcgccatcga tggtgatggc ggcgccgtgt acacgttccg
      121 ctagaccacg tcgaatacga gatcgccgtc agggttgacg gtgtattcga acagattgcc
      181 ggccgacgtg atcttgacct tcgcgttctc gccaatgagc gcgttcgcga gaatcacgat
      241 gcgtccgatg ttcgagccga tcgtcctctg cacgggcggc ggcaccgagc ccgacgtacc
      301 gtcgaagaca gcggactcga cttctgaaat cttgtcgcgg cgaatgttca cgtacgtcac
      361 ggacactttc gagagcgtca cgacctcgag cgtgtagcct gcaccctgag caaacgaccc
      421 catgtatggc atctcgtccc cctgttcgag ttggacgaaa atagtaacat cgtgatgcgt
      481 gcgcacgctg gtggggacat gcgttatcgc gatcgggtgt ggcagcgggt cgtcgacgcc
      541 ggacgccagc cgcaacgatt cgctgcagct cgatgcggac atcgacggca acgcaatcga
      601 tgcaccggac gcgaacacgg gaagcggtgt gcgtgtcggt ggcactgcgt tcggcatccg
      661 cgcacccgtc gaggtcacac tgaccgtcga cagcgcgagc catcgcatga tcgtcaccga
      721 ggacggcgcg ttccgctttc cgcgcaagtt tctgtccgga acgtattcgg tcaccgaaac
      781 cagcgcggac tgcgtgatcg agaacggcac cggcacgctt accgggagtg aggtcacgaa
      841 cgtgtcgttg atctgccgtg gcgtggtcga gctcggctcg atcgcgttcg cgccgaacgt
      901 cgtgcgtctc gcgaccacgt tcgatccggg tgctccgctc acatacacgg cgacccgacc
      961 gttcttcgcc gatgcgaccg acacgatcac ggtgaccccg accgcgctct atcccgccct
     1021 cgcgtcgctc gctatcaacg ctgcaccggc gagcagcgga cagccatccg tgcagccgta
     1081 caacgcgcag acaccggttg cgctcgcgta cggctcggcg ttcgcgaaca tctatacgtt
     1141 cgcgaccacg cccgaggtgt cgttcgaagc gtacgtcaag gcgtctgaca cggcgatgca
     1201 ggccggcttc ggaagcggcc agtcgacagc ctgcatgggc gcgacgaaca agaacggcaa
     1261 cggcgtcgca ctgagcggcg acacgctcgt tgtcggatcg ccgtgcatca acgtccgcgc
     1321 cggcgcggcg tatgtctacc gccgcacggg cacgacttgg gcattcgagg ccaagctcac
     1381 cccgaccgtc gcagatatga atgacttgtt cggaacgtcg gtcgcgatcg acggcgactc
     1441 gatcgtcgtc ggtgcgttcg gtgaagcgag tagcaacggg accgcgaccg acaacgccgc
     1501 accgaacgcg ggcgcggcgt acgtgttcac gcgaagcggc acggcgtggt cgcagcaggc
     1561 gtatctcaag gcgacaaaca tcgacgcggc cgatcggttc ggtacgtcgg tcgccatcgc
     1621 gggcgacacg gtcatcgtcg gtgcgcccgg tgaatcgagc agcggcaccg gcatcaacgg
     1681 cacggcgaac aacactgcgt ccgcggccgg cgctgcctat atcttcacgc gcagcggcac
     1741 gacgtggtcg cagcaggcgt acgtgaaggc ctcgaacacc ggcgccaacg acgccttcgg
     1801 caccgcggtc gcgatcgcga gcaacacagc ggtcgtcggc gcgccgaacg aggacggtgc
     1861 gacgggcgat gacggtgcgg tttacgtgtt cgtgcgcaac ggcaccacgt ggtcgcagca
     1921 aggctacttg aaggcatcca atgccggcgc gtccgacacg tttggcacct cggtcgccgt
     1981 cgatggcaat ttgctaatcg gtggcgcgcc tggcgaggac ggcaacggct cgctcgcgga
     2041 gaacgactcc ggtgccgcgt acgtgttcac gcgcaacggc acgacgtgga cggaggacgc
     2101 gatgctgttc gcgaacacgc gcgtgcagag cggcgggttt ggtcaggcgg tcgcgatcga
     2161 tcgtacgcac gccgtcgtcg gctcgcgact cgcgggcaac ggcatcatcc aggcgtaccg
     2221 gcgcagcgcc accgcatggg acacgatccg cttccgcaat gcgccgaaca tggatgtcgc
     2281 cggcctcgac gagttcggct ccagtgtcgc ggccgatggc gacacgttcg cggccggcgc
     2341 gttcaaggaa gactcgaacg cgacgaacgt cggtggcaac ggcgccgaca acagcgcgac
     2401 ggacagcggc gccgtgtacg tgtttcgcta agcagcgtcg agctcgatca cagacctgag
     2461 cgcaaaggaa cgtctacgct cgaacgccga atggcgcctt ccacgccgca cgacgtgttc
     2521 cagatcgaac gccaagacgc cgaacagagc caaggtcgcc accagagagc ggtcttctaa
     2581 cacgctctgc accagcgatc gcgcgagcag aacgcgatca agagacgcgc ttggcgttct
     2641 tggccggctt gggatcttgg cgttcctctg ggagccgcga agcggcagac gcgggctcct
     2701 tcgacggcgg cgttctgcat ctacgcagcg tcgagctcga tcacgaaccg tgcaccgggc
     2761 tcggcgtgct cgaaccagac gcggccgccc atcgcctcga cgtggctctt cacgatcgcg
     2821 aggccgagac cggtgccgcc ctggtcacgg ctgcgtcccg gatcgacgcg atagaagcgc
     2881 tcgaagatgc ggtcttcgtg acctgctggt atgcccgggc cggcgtcggc gatcgcgagc
     2941 ctcactttgc cctcgcgctt ggtcgcacgc acactgaccg gcgcaccgtt gccgtacttg
     3001 atcgcgttat cgatcaggtt ctggacgacg tgctcgaggc cttcgcgggt tccgaggact
     3061 gcgatcgcgc caacgtcgac gtcgatctgc gcgctcggtg tcacgccctt cgcggtgcgc
     3121 accgcgtcgt cgacgacctc gagcagcggc accgacacgc gctcgccgac gacggccgca
     3181 cggccgccga gcgtgtcgag cacgagtagg tccgacacca tgtcggagat gcggcgcgcg
     3241 ttgcgatgga tcgtggtgat gaactctttc gacagctcgg cgtcgatgcc gccaccgagc
     3301 agcgtctcgg cgtagccgga gatcgacgtc accggtgtgc gcagctcgtg cgcggcgttc
     3361 gacaagaagt cgcggcgcac cgcttcgagc gcgcgcatgc gcgtgacgtc gtaaagcacc
     3421 acgatgaccg ctggccgtga cagctgcggc ggcgttgccg gtaagccgac ttggttctgt
     3481 tcgccggtcg tcttgccagg cgcgcgcaac ggccgcgcac tcgcgcgcac ggtgcggccg
     3541 accagctcga gctcttgctc tggctgctcg cccacgcgtg ccgcatcgac gagcttggca
     3601 agcgcggccg gcagtgagtc gccgatcagc ggacgcgcgg cggtattggc aagcacgatc
     3661 gtgccgtccg gatcgacgac gacgacaccc tcgacaaggc caccgagaac gaccgacaac
     3721 agatcgcgct gctcggtcag ctcgttgacc cgggacttca cctcgcctgc catccgcatc
     3781 atcgcgcgcg cgagcacgct gagctcgccg cctgcatcgg tcgccgcgac cggcggcgcg
     3841 acgtcgtagt tgccggccgc gagcgtctcg gcggtgcgtg tcatcgactg cagcggcttg
     3901 gtaatcgcgc gcaagaagat ccacgacagg aacaacgcgc cgagaaagcc gaagccaaag
     3961 ccgacgatca gtcgattgcg catgcgcgcg cgggcatcga cgatgtcgcc gagcggcacc
     4021 gcgagccgga tcacgcggcc gagctcggcg ggcaccgcga cgagatactg cttcggctcg
     4081 tcgggccgca gctgccgcac cgcgcgcgcg acctgcccct tgcgcgcctg cgcgacctcc
     4141 cacgcgctac cgatgttctc accgacgctc tggggctcgt gcgaatcacc ttgcacaacg
     4201 ccatccgcac cgacgatcgt gagccgcgtg ccggtgacct cggcgagccg cggcgtcaat
     4261 cgatcgacgt ggcccgcgat cgtcagccac ttcgataccg ccgtgccctg atcggagagg
     4321 cgcttgtcga gcgcctcgag caagtcgtcg gcgagcgtgc gctcgatcca gtacagcgtg
     4381 aacgcgccga ccacgacgac gaggacgcag aacagaccga tcagcttgta gcggagcgag
     4441 agcttcacta ggcgtcgcgt cgcgctcgat agccgaagcc gtgcacggtc tcgatcacgt
     4501 cagcggccgg gcctaggctg acgcgcaacc ggcgcacgtg gacgtcgacc gttcgcgtgt
     4561 tgatgtcacc gcgcatctcc cagacttgct cgagcagctc ctcgcgcgag aacacgcgtc
     4621 ccggatcgga cacgagcaac tggagcagct tgtattcgag tgggcgcagc tgcgtcggcg
     4681 cgccctggat gcggacgtca tgcgtgaccg gatcgagctc gatcggcccc accttgagcg
     4741 acgtgccggt gctctgcgat ggccgcgcgc ggcgttcggc aagccggttc gcgagcgcgg
     4801 tgacacgcaa cacgacttcg cgaacgacaa acggcttgac gacgtagtca tccgcgccga
     4861 cctcgagccc taaaatccga tcttctgctt cgccttttgc tgtcagcatc agcacgccga
     4921 tgtcagccag ggggctttcg ccccggatcg tcttgcagac ctcgtagccg ctcgtgtcgg
     4981 gcagcatgag atcgagcacg acaacatcag gcggatgctt acgaatcgca gcgagaccat
     5041 cgttaccggt tccacacgct tcaaccgtga aacctgcttg ttgcaagttg aactcgagta
     5101 gccgcgccac atcgcgctcg tcttcgatga tcaccacccg gcgttccatg ccactcgtta
     5161 taccgcgatt gaatcgcaga taggtgtcgg cgtacgctcc tgccatgcgc cagcttgccg
     5221 tcggtctcgt agtactcgca gccgcgtgct cgtcgaagaa ggaggcgcct gcgaacacgg
     5281 cgcccaaaga ggtgcacgtg gccgctgcgt cggatctcac gcaggcgttc accgaagtcg
     5341 gcgcagcgtt cgagaagaaa acgggtatca agccgaactt caacttctcg tcgagcggcc
     5401 tgctcgcaaa gcaggtcgag caaggcgcac cgctctacct gttcgcggcg gcgaacaagt
     5461 cgttcgtcga ccaggtcgtc aaggcgggca agtgcgacgc cgcgactgca cagctctact
     5521 cgcgcggtcg gctcgtgatg tgggccaagg gcggaccgcc cgcgaagatc accgatctcg
     5581 tcgatccgaa gtacaaacgg atcggcatcg cgaaccccga ccacgcgccg tatggcaagg
     5641 ccgcgaagca ggcgctcgag aaggccggca tctacgagca ggtcaaggat cgcctcgtcc
     5701 tcggcgagaa catctcggcg acgatgacct acgcgaagga aggttctgtc gacgtcgcgt
     5761 tcgtcgcgct atcactcgcg atctcgaccg acgcgcagat gtattcgcca gtcgagctcg
     5821 acctccacga cccgctcgag cagacgcttg tcgtgtgtgg caagggccct gaagccgatg
     5881 cggcgaaaca gctggccgaa ttcatcatca gcacagacgg tcgcaccatc atggagcact
     5941 acggcttttc gacgactggt gttatgacgc agaagtaatg cgaccggaga ccatcgcagt
     6001 caaagcgatc gagatcgatg aagccacggg cgcgctcgtc ccgccgattc atctctcgac
     6061 cacctatgta cgcgacgccg agtaccggct gatcgcagat cgcgactaca cgcgcgacaa
     6121 gaacccgacg ccgctcggcg ccgagcacgc gatcgcagcg ctcgagggcg gcgcccgcgc
     6181 gctcgtattc tcgtcgggga tggcggccgc gacgtcgctc gtgcgctcgc tgacaaagcc
     6241 cggtgatcac gtgctcgcgc caaaggtcgc ctactacgcg ctgcgcggct ggttcgagcg
     6301 gttcaccacg aagtggggca tcggcctcga cttcgtcgac atgacgaatc tcgatgccgt
     6361 acgcggcgca cttcgcgaca ccacgcgact cgtctggctc gagacgccgg cgaacccgac
     6421 ctgggacgtc accgacatcg ccgcggtcgc cgagctcgcg catcgcaagg gcgcactggt
     6481 cgcggtcgat tcgacgtgcg cgacgcccgt gcacacgcgg ccactcacgc tcggcgctga
     6541 tctcgtcatg cactccgcga cgaagtatct cggtggccac tccgacgtgc tcgccggcgc
     6601 gctcgtcacg cgcgaggaca acgcggcgtg ggcagagatc catcgcacgc gtcacgacga
     6661 aggcgccgtg atcggaccgg tcgaggcgtt cctgctcggg cgtggcattc ggacgttgtt
     6721 cgcgcgggtc gagcgccaga gccgcaccgc gctgtcactc gcgacccggc tcgccgaacg
     6781 cggcgtgacg gtctgctatc ccggcctgcc gagccatccc cagcatgcgg ttgcggcgaa
     6841 gcagatgacc ggcgggttcg gcggaatgtt gtcgatccgg gtgcccgatc cgctcgcgct
     6901 cgtgaagaag ctgcgcgtgt ggatgccggc gacgtcgctc ggtggcgtcg agagcttgat
     6961 cgagcatcgc gcgagcgtcg aagggccgac gagcccgacg ccgaaggatc tgttgcggct
     7021 gtccgtcggc ctcgagcacg aggacgatct gttcgaagat ctggtgcagg cgctgtgaag
//
LOCUS       DASUFX010000193        16684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_168159,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000193 DASUFX010000000
VERSION     DASUFX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16684
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168159"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1257)
                     /locus_tag="VFV99_07625"
     CDS             complement(142..1257)
                     /locus_tag="VFV99_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059213.1"
                     /translation="MRLDIDRNDDWYESEESTRKGMVHELRRIRRRFIVRPWPVLALA
                     ALITGVLTYKLVTRKRTYGAEVVLALRDGSLGTRDRVGMPVSELKEFVGSVLIPDNKL
                     EELIERRNMMRLRNRLGMNWAVAELRTQFEIDVWRNSYIYFDPDLPNRDPSARIGISY
                     YDDDPDSAFSLAHDLASIVVESVREHRMEYAGQIATSIASDRAGLSKRLNALELERTE
                     VMVAFSKARRANKQGVAQALNLRLTEIDEQQKDAEKMMTELTTSQDAIADRIAAAGLD
                     LVIEIVSETRPARPPSRGLLITVLVVIVGIGSLIGSALLIGAFDSRVHDVEDVERLGL
                     PVLGHVPGFPGDRVGSLEARGVQRTRIPSFRRWRSQR"
     gene            complement(1260..2435)
                     /locus_tag="VFV99_07630"
     CDS             complement(1260..2435)
                     /locus_tag="VFV99_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5059214.1"
                     /translation="MLRVLAITKIFPNAAEPLAAPFNRQQFSALREHCDLNILATIPW
                     FPGAALLAKWSSAGRLARVPRRERIHGMDVSHPRTLFLPKLAMATWGPLYAASIAPAV
                     APYRNKVDLVFGTWAYPDGFASVIAARLLGVPCVVKLHGSDINLIAKEPGPRRMTSWA
                     LPQAARVVAVSRALADEVVAMGVERERVSIVMNGVDGELFKPGDRNAARAELGLPDGP
                     LAVYVGNLKPEKGVLDLGAAWASVLRHVPNATLVVVGDGPQHTELSALTKPHGERVRL
                     IPRQPLERVPAYMAAADILVLPSHHEGTPNVVLEALASGRRVVATSVGGVPDLITSHT
                     VGVLVPPRDPDALADALVLALREPYDAIEVARLGARGGWAASAAALHAVLKEAAKVC"
     gene            complement(2435..2743)
                     /locus_tag="VFV99_07635"
     CDS             complement(2435..2743)
                     /locus_tag="VFV99_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5059215.1"
                     /translation="MPVPAFDVSRSSGSTILTVRGSLDINTASLLGEEIDRIVVDKPK
                     LVIVDLSGLDLIDSSGVAALVKLYKAVRSAGGNVTISGVRDQPLAIFKLLRMDRVFNL
                     "
     gene            complement(2724..3206)
                     /locus_tag="VFV99_07640"
     CDS             complement(2724..3206)
                     /locus_tag="VFV99_07640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059216.1"
                     /translation="MAATIRLSVPGTLRYRDVAVRIVAEAARLVSCSAQRAPNDPLDH
                     DVRDPFDTAVVSAFSEIFNNVAIHAYKRKSGGVIEIVITPNDREIVIELKDTGHPFDI
                     DEVPPLPSELDDESLPEGGMGIHIAKTMLDEVTYEPGPPNLWRLCKRLPGGAHASTRV
                     "
     gene            3252..3986
                     /locus_tag="VFV99_07645"
     CDS             3252..3986
                     /locus_tag="VFV99_07645"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5059217.1"
                     /translation="MRRSVRLAKRAIDMLGAAIGLAVTAPLMPIIAAAIYLDSPGPIL
                     FRQRRAGSLKKISNENGIKKLQFDEFHMHKFRTMIPNAEAKTGVVVAGADDPRITRVG
                     RFLRKSRLDELPQFWDVLRGKMSLVGPRPERPELLQDLSYAIPLFEERMRDVKPGITG
                     LAQISLGYTGSIPEGSEIGKLASTLQNPWGFEESKGSLADDMRIKLLYDLAYTASLER
                     FDTFLMTELGIIFKTPLVMIKMTQGR"
     gene            complement(4002..4904)
                     /locus_tag="VFV99_07650"
     CDS             complement(4002..4904)
                     /locus_tag="VFV99_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059218.1"
                     /translation="MGTKILLALIVMAGVASAQPAPPKITIGIYAPSVEFGTAQARLT
                     YVQGLAKAIEQSTSIKTDAYAYASMSALKKANVDYAIVDGPCFATNLGWKLLANATID
                     GGTSRTYALWSSAGNTMQSLRGKKLAYVQTGCNDDGFIFNAMLETEVDPTFFSGRPPE
                     NDLTAAVAAVQSYKTAQGVFAPASAVKSLTKVFDTGSVPNPAFVEVNAKMPNNISAAV
                     GAAVSGYGGSGAISGWTKPNRDPYQSLASRFTPNRKGGVFASPDAVRLNPNDVLAAVP
                     SLNEPFFVAVRHHFVRGPARMGGE"
     gene            complement(4894..7443)
                     /locus_tag="VFV99_07655"
     CDS             complement(4894..7443)
                     /locus_tag="VFV99_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059219.1"
                     /translation="MRALFVAATIMIVGQYAMASVEDDLREGDKYYEDGDYKKAAASY
                     DRAINSAPAQVSAEAYGKRAGLFIILKDFKGGLDFIEQRAKRQYPNAPEVLEQEALML
                     WETDRRDDAIKVAERVVKSKPSSFTNQQIIGEYYAGRDSVKTAAAFEAYLQSRPIEVE
                     ERDSSRTGKYSVLPHIRLGFAYLSNSKSVLNDGDEQRAKQLYTKAMEQFEYVQRKLGK
                     KPGAQTNADNGLCAVYSGLNRWDQAISVCERVIQDPRRIDANGSAWYNLANAYLARKQ
                     MKKARAAGNEFTRLRKNEARGFMLIGDTFFEEKDWNNALDQYLRAEKLLRPNQTREQV
                     LLSIRLGKTYRRLPAPASGPNPNLALAIEKLNNAWAANPGSLELAAELGTAYLAGKQD
                     SKAMALTDKVVATPEFAKAPSDEKGALFVISGQALYNQKKLKEARARFEAARQERAGD
                     VQITRYLVLTMNEQAFEQGKNYKEALGYLDEALKLEPQNTTTLTNIAVLAIERGDCEN
                     ANRMLQKLNGVGGRDVVLTQRLIGRSYMCFNKPDVAKASAAFAAAESEAKKAGAQLAL
                     AEIYTEWAPLTWDKDLEGAVDKLELAVQASAQDPEIGSAAKRNLALALYRRGWQNMRN
                     GKQTDAASDFERAVRNTDVLKGIEPLSFEFSYALALLDANRYQDAAKAFKSLATKGNT
                     AAYLKGNYGKVGTQYFSAYAAYRSAQSAAARSAACGELAKLEPTVGGKASEITASCWE
                     YAAYDHWRAGSTGAAMSALKNAERAAGAEQKRRLNNNRTVANGLDKGSLNELEALGGN
                     PAEALVNLGIVYEKIGKPKDAYEAWVRAAKAGARAGNLQKWIEAKKRIYGY"
     gene            complement(7440..9167)
                     /locus_tag="VFV99_07660"
     CDS             complement(7440..9167)
                     /locus_tag="VFV99_07660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5059220.1"
                     /translation="MAKRGGPSISVKMILTTTLLIVVTGVGAGLLNVMNLRRAFDESA
                     ERRVELFQSLRQQFGQIGTPLFARATEALVLEKGRDKDILTLVKNAVAGDTRESNGKK
                     DYVLKLAYVLDLNQKVIAHCHEGTGSTADCNVFDEHAPVSPALGQITVESWNKALAAW
                     KANAADPAKAGNVLVEFDLASGNSNYKIFAYPIFSGDPPASAAGTIAADPPISRQGYV
                     VLGYDLAPVEWFKAEANEQKAAAVTKAALYTGAVGALFALIGTVLAIFQGLSISKPIK
                     QLAWKADQIARGDLDARVDVNTGDEIGMLGENFNFMADQIAILLQQTAEKAKIEQELE
                     VAKAIQETLVPSDAPVKKSTLTFAGFYQPAAQTGGDWWTWAELVGGKILVVIGDVTGH
                     GVPSAMITAAAKASCDVARYVHNDDVTVTTLLEIMNHAIFESAQRRFVMTCFASIIDT
                     KARTITYANAGHNFPYLFRPGEGKGEFGSLMIRGNRLGDDRNSKYEAKTTECAIGDVL
                     VWYTDGIVECENEAGEEYGEKRFRASVRKAAALDAGEMRDAIIADAGAFFGEAIRKDD
                     ITMVVGRIH"
     gene            9218..9772
                     /locus_tag="VFV99_07665"
     CDS             9218..9772
                     /locus_tag="VFV99_07665"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5059221.1"
                     /translation="MDNTSPALTQFLTEFKRVNNAIVDSYFIVDTERRIVDFNRAFYA
                     LLPRSVARGLKGKHCYEVLELNICANECIAQQCWADNRHVRLDEISGNIAGEAQKLRF
                     ILSAVPITDEAGAHVGALEIQRNVTDEAVVQVKYQEMLEKEARERERLANQVRMRTKE
                     LLETNQLLLKTQKELLAYKKGLTV"
     gene            9811..11202
                     /locus_tag="VFV99_07670"
     CDS             9811..11202
                     /locus_tag="VFV99_07670"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5059222.1"
                     /translation="MSPLDWRAVGLVLVAACYSASPALNVPCGPNGECPSGQQCDTAQ
                     TPPTCVLTLMDASVAVEDGPPPIDAPPLECTVSKDCTDAGTPVCDDSQHVCRACVADA
                     ECDGVCTEYDGQCVGNGQAIFVSPTGTDGGNCMASAPCRTFSYAFQQLTANRRVIRVG
                     DGMYMRTGNPVLDVTDTGGRIVFSGEDRDPAGAVMTAVANSTTTPPIVSTDPGTDVIV
                     EGITLTNADTDAVRSSGTFLVSRVAITNNGDRGIQHQPTSSAAELHVWDSLIAGNSGD
                     GVYADRGSAEVLRSTVHNNSDGGMTFDGLTSVKVVGSIITRNGNGASSYGGIRTINVG
                     VQDAVIAFDTIAFNRCSGTAAAPGLSTADVINVTNSIATDNTNDATVPRQICDTCTSS
                     YSLFSGNVPPGTGNIAGPAQFVDAVGNNFHLMATSPARDAADPGAMPGHDIDGNSRPL
                     GAGFDIGADEIMP"
     gene            11202..12722
                     /locus_tag="VFV99_07675"
     CDS             11202..12722
                     /locus_tag="VFV99_07675"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5059223.1"
                     /translation="MRLLALLAVAACAHTPNLPLRPDPSPPAGSTTETITARDGTQLL
                     ARHWAAVGAGQPGPDGSVARGGDARGVVVIMHGLKDYSARYASLASRLAAGGYSVYAF
                     DLRGHGRSAGPRVAPDAWTDYVDDLDRFLTTVEQREPGKPIFLFGHSMGGAIAARTAE
                     LHQPALAGLVLSGPALAIDAAPLLISATRLIAVMNPRAPALKLPNGDFSSDPAAATAM
                     DKDELVSQSPGPARTAAGLVDGMRAIWADVDRLTMPLLALHGTADRLTAPSGSRALVE
                     HAPAQDKTLRIYAGFFHDLLHEPKRDQVEADIHAWLDAHTGGAAVAAPPIYAEHLNGD
                     PRGWTQAIEFAGGISRGFDEKVTRFGGELAINLARPRPFGWHGAFTARLANTRYAVAL
                     KPLGVAARFGGAVFGLSGGGALLTDAHVGVAAGGWYEQPAGPLHLGIRADYEHAFFAG
                     ARNVGFLVGSLRFGGDRAYWPHARAGVGPIVSGGYECQGDTCGFTAMLGLQLYGAD"
     gene            complement(12935..15334)
                     /locus_tag="VFV99_07680"
     CDS             complement(12935..15334)
                     /locus_tag="VFV99_07680"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX5059224.1"
                     /translation="MVDDARSFALDLLEKLYVELAKFPGVATRNDHYLALAYAVRDRL
                     LHRWVDTARAVLTNQQRTVIYLSAEYLIGPQLGSNLMALDLEETVREATKEVGLSLDD
                     LLEHEEEPGLGNGGLGRLAACYMDSLATLGIAAVGHGLRYEFGIFDQDIRDGWQVEHT
                     DRWLRHGFPWEIRRYDVEHSVGFGGYTEHGETGATWHPERIVKGVPYDVPVAGYGTPT
                     TNFLRLWSAVAAEEFDLDAFQIGEYWRAVEAKLRSENLTKVVYPNDTSPAGKQLRLEQ
                     QYFFVSCALQDCIRLLTIGGAAIHQLADLIAIQLNDTHPTLAIPELMRLLMDVHGLGW
                     DAAWAITSKSFAYTNHTLLPEALETWPLPLVQRLLPRHLEIIYEINRRFLDEVSAKFP
                     GDDARIRRMSLIGEDGEKQVRMAYLATVASHHINGVAELHSRLLRETVLRDFADMWPE
                     RFTNVTNGVTPRRFVALANPRLADVINDAIGDDWLRDLERLRELERFADDAAFQERWR
                     AVKLANKRDLSDHLGGVGGAFDPAALCDAQCKRIHEYKRQHLNLLHAIVCFDRLCVGD
                     DSDVPRTIVLAGKAAPAYRAAKLIIRLAHGIAEVVANDPRTRDRLRIVFVPDFSVKKA
                     QRIYPAADLSEQISTAGMEASGTGNMKFTLNGALTIGTLDGANIEIRDAVGAENFFLF
                     GLTADEVIKKKREGYRPIDVLRHDNELANAIELISSGLFSRGDRGTYAQLVSDLVERD
                     PFLVLADFRAYADAHRAVAAAWRDPARWTRSSILNVARAGYFSSDRSVREYAQKIWRV
                     G"
     gene            complement(15340..15636)
                     /locus_tag="VFV99_07685"
                     /pseudo
     CDS             complement(15340..15636)
                     /locus_tag="VFV99_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(15703..>16684)
                     /locus_tag="VFV99_07690"
     CDS             complement(15703..>16684)
                     /locus_tag="VFV99_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059225.1"
                     /translation="RDHIAQLPADLAKLFELELRISEGPQHAWPALVDAARAACTEKT
                     AAIDLAYSLLRAEPALGVIAARACIGTLNVDDPDLMLELVEDARDRLNVRPDDPAWDV
                     LEALTDKTTKKPTEDDAKLRESLAESSARINELERALAAVRTELASARTRPAAELMRA
                     PEQRGVLDQRVAELEALIREGQAERRDLRKQIESAASEDASTRREGPRARRVTLEESD
                     DDVGDDVELGARGIAIPRFERRAIDALADVPAAVASETMRTVGTLAAGDGFAWRSVKQ
                     AKDMVRPVLMARVGIHHRVLFRVEDGALDVIDLITREQLLTTLKRLRANR"
BASE COUNT         2730 a         5457 c         5446 g         3051 t
ORIGIN      
        1 acgactggta gttcttcggg cgcacgagcg cgaacgcgac cgacagtgcg ccgcctgcga
       61 tcgcgaacag gaccacgagc caccagtacc gtctggtctt gcggccgagg tcgacaactc
      121 gttgcaatct gtctcgtggt gtcatcgttg ggaccgccat ctcctgaacg aaggtatgcg
      181 tgtacgttgc acgcctcgtg cctcgagcga tccgacgcgg tcacccggga atccgggcac
      241 atggccgagc acgggcaatc cgagccgctc cacgtcctcc acatcgtgca cgcgcgagtc
      301 aaacgccccg atgaggagcg ccgatccgat cagtgagccg atcccaacga tgacgaccaa
      361 gaccgtgatg agcaggccgc ggctcggtgg tcgagccggt cgcgtctcgc tcacgatctc
      421 gatcacgagg tcgagcccag cagccgcgat gcgatcggcg atcgcgtcct ggctggtcgt
      481 gagctcggtc atcatcttct cggcatcctt ctgctgctcg tcgatctcgg tgaggcgaag
      541 gttgagggcc tgtgcgacgc cctgcttgtt cgcgcgacgc gccttgctga acgcgaccat
      601 gacctcggtg cgctcgagct cgagcgcgtt cagtcgcttc gagaggccag cacgatccga
      661 ggcgatcgac gtcgcgatct gccctgcgta ctccatgcgg tgctcgcgta ccgactccac
      721 gacgatcgac gcgaggtcgt gtgcgagcga gaacgcagag tcgggatcgt catcgtagta
      781 gctgataccg atgcgcgccg acggatcgcg gttcggcagg tcgggatcga agtagatgta
      841 cgaattgcgc cagacgtcga tttcgaactg cgtacgcagc tcggcgacag cccagttcat
      901 gccgaggcga ttgcgcaacc gcatcatgtt gcggcgctcg atcagctctt cgagcttgtt
      961 gtcggggatc agcacgctgc cgacgaattc cttgagctcg ctgaccggca tgccgactcg
     1021 gtcccgcgtg ccgagcgagc catcacgcag cgcgagcacg acttcagcgc catacgttcg
     1081 cttgcgcgtg acgagcttgt acgtgaggac gccggtgatc agagcggcga gcgcgagcac
     1141 gggccacggc cttacgatga aacgccggcg aatccgccgg agctcgtgca ccatgccttt
     1201 gcgcgtcgac tcttcggact cgtaccaatc gtcgttgcga tcgatatcga gtcgcatggt
     1261 cagcagacct tagcagcctc tttcagtacg gcatgcagtg cggcggcgct cgctgcccaa
     1321 ccaccgcgtg caccgagtcg tgcaacttcg atcgcatcgt aaggttcgcg taaggcgagg
     1381 acgagcgcgt ctgcgagcgc atccggatcg cgcggcggca cgagcacgcc gaccgtgtga
     1441 ctcgtgatca gatcgggcac gccaccgacg gaagtagcga cgacgcggcg gccactcgcg
     1501 agcgcttcga gcaccacgtt cggcgtgcct tcgtgatggc tcggcaacac gaggatgtcg
     1561 gctgccgcca tgtacgccgg cacgcgttcg agtggttggc gaggaatgag gcgcacgcgc
     1621 tcaccgtgcg gctttgtcag cgcagaaagt tctgtgtgct gcggtccatc accgaccacg
     1681 acaagcgtcg cgttcggcac gtgacggagc acgctcgccc acgccgcgcc gagatcgagc
     1741 acgcccttct cgggcttcaa gttcccgacg tacaccgcga gcggtccatc gggcaggccg
     1801 agctcggcgc gcgcagcgtt gcgatcgccg ggcttgaaca actcaccgtc gacgccgttc
     1861 atcacgatcg acacgcgttc gcgttcgacg cccatcgcga cgacttcgtc ggcgagcgcg
     1921 cgactgaccg cgacgacgcg tgccgcctgc ggcaacgccc acgacgtcat gcgccgcggc
     1981 ccgggctcct tcgcgatcag gttgatgtcg gagccgtgca gcttcacgac gcacggcacg
     2041 ccgagcaagc gggccgcgat caccgacgcg aagccgtcgg gatatgccca cgtcccgaac
     2101 acgagatcga ccttgttgcg atacggcgcg accgccggcg cgatggacgc ggcgtagagt
     2161 ggaccccacg tcgccatcgc gagcttcggc agaaacagcg tccgcggatg cgagacgtcc
     2221 atgccgtgaa tgcgctcgcg gcgcggcacg cgtgcgaggc gtcctgcgct cgaccacttc
     2281 gcgagcaacg ctgcgcccgg gaaccacggg atcgtcgcca ggatgttcag gtcgcaatgc
     2341 tcgcgcagcg ccgaaaactg ctggcggttg aacggcgcgg cgagcggctc ggcggcgttc
     2401 ggaaagatct tcgtgatcgc gagtacgcgc agcactacag gttgaagacc ctgtccatcc
     2461 tcagcagctt gaagatggcg agcggttgat cgcggacacc gctgatcgtc acgttgccgc
     2521 cggcggagcg cacggccttg tagagcttga cgagcgcagc gacgcccgag ctgtcgatca
     2581 gatcgagtcc tgacaggtcg acgatcacca gcttcggttt gtcgacgacg atgcgatcga
     2641 tctcctcacc gaggagagat gcggtgttga tatcgagcga gccacgaacg gtgagtatcg
     2701 tggagccgct cgagcgcgaa acatcaaacg cgggtactgg catgggctcc tccagggagt
     2761 cgtttacaca agcgccacag atttggcggt ccgggttcgt acgtcacttc gtcgagcatc
     2821 gtcttcgcaa tatggatgcc cattccgcct tccggcagcg actcgtcatc gagctcgctc
     2881 ggcagtggcg gcacctcgtc gatgtcgaac ggatgaccgg tgtctttcag ctcgatgacg
     2941 atttcgcgat cgttcggcgt gatcacgatt tcgatcacac cgccgctctt gcgcttgtac
     3001 gcatggatcg cgacgttgtt gaagatctcc gagaatgctg acacgacggc ggtgtcgaac
     3061 ggatcgcgca catcgtgatc gagaggatca ttcggcgcac gctgtgccga acacgatacg
     3121 agtcgtgcgg cttcggcgac gattcgcacc gcgacatcgc ggtaacgtaa cgttccgggc
     3181 acgctgagcc gaatcgttgc ggccatgtgt ggactctata gcaggctcat tgtgtgcatg
     3241 gcatgccgct catgcgtcgg agtgttcgac tcgccaaacg agcgatcgac atgctcggtg
     3301 cggcgatcgg gctcgctgtg acagcgccgc tcatgccgat catcgcagca gcgatctacc
     3361 tcgattcgcc gggtccgatt ctgtttcgcc agcgtcgcgc cgggagcctg aagaagatct
     3421 cgaacgagaa cggcatcaag aagctccagt tcgacgagtt tcacatgcac aagttccgca
     3481 cgatgatccc gaacgccgag gcaaagaccg gcgtcgtggt tgcgggcgct gacgatccgc
     3541 gcatcacgcg tgtcggcagg ttcttgcgca agtcacgact cgacgagctc ccgcagttct
     3601 gggacgtgct gcgcggcaag atgagtctcg tcgggccacg gcccgaacgt ccggagctgc
     3661 tccaggatct gtcctatgcg attccgttat tcgaggaacg catgcgcgac gtgaagcccg
     3721 gcatcaccgg actcgcgcag atctcactcg gctacaccgg ttcgattccg gagggcagcg
     3781 agatcggaaa gctcgcatcg acgctgcaga acccgtgggg cttcgaagag agcaagggct
     3841 cgctcgccga cgacatgcgc atcaagctgc tctacgacct cgcgtacacc gcgtcgctcg
     3901 agcggttcga tacgttcctg atgaccgagc tcggcatcat cttcaagacg ccgctcgtga
     3961 tgatcaagat gacgcagggc cgctagtcag cggccaccgc gttactcgcc gcccatgcgc
     4021 gcagggccgc gcacgaagtg atggcggacc gcgacgaaga acggttcgtt gagtgacgga
     4081 acggcggcga gcacgtcgtt cgggttgagg cgaaccgcgt ccgggcttgc gaacacgcct
     4141 cccttgcggt tcggcgtgaa gcgactggca agcgattgat acggatcgcg gttcggcttc
     4201 gtccagccgc tgatcgcacc cgagccgccg tagccagaga ccgcggcgcc gactgcggcg
     4261 ctgatgttgt tcggcatctt cgcgttgacc tcgacgaagg ccgggttcgg caccgagccg
     4321 gtgtcgaaca ccttcgtcag gctcttcacc gcgctcgccg gagcgaacac accctgcgcg
     4381 gtcttgtacg actgcaccgc cgcgacggca gccgtgagat cgttctcggg cgggcggccg
     4441 gagaagaacg tcgggtcgac ttcggtctcg agcattgcat tgaagatgaa gccgtcgtcg
     4501 ttgcagcccg tctgcacgta cgcgagcttc ttgcccctga gcgactgcat cgtattaccg
     4561 gcgctcgacc acagcgcata ggtgcggctg gtgccgccgt cgatggtcgc gttcgcgagc
     4621 agcttccagc cgaggttcgt cgcgaagcac ggaccatcga cgatcgcgta gtcgacgttc
     4681 gccttcttga gcgcgctcat cgatgcatac gcatacgcgt cggtcttgat cgaggtgctc
     4741 tgttcgatcg ccttcgcgag gccctgcacg tacgtcaggc gtgcctgcgc ggtaccgaac
     4801 tcgacgctcg gtgcgtagat cccgatggtg atcttgggcg gcgccggctg tgccgaggca
     4861 acgcccgcca tcacgatgag cgcgagaagg atcttagtac ccataaatcc tcttctttgc
     4921 ttcgatccac ttctggaggt tgcccgctct tgcgccggcc ttcgccgcgc gtacccacgc
     4981 ctcgtacgcg tcctttggct tgccgatctt ttcgtagacg atgccgaggt tgacgagcgc
     5041 ctcggccgga ttgccgccga gcgcttcgag ctcgttcagc gaacccttgt cgagaccgtt
     5101 cgccaccgtg cggttgttat tgaggcggcg cttctgctcc gcccccgccg ctctctcggc
     5161 gttcttgagc gcgctcatcg cggcccccgt cgagcctgcg cgccagtggt cgtatgcggc
     5221 gtactcccag cacgacgccg tgatctcgct cgccttgccg ccgaccgtgg gctccagctt
     5281 cgcgagctcg ccgcacgcgg cggagcgcgc ggcagcactc tgcgcgctgc ggtaggccgc
     5341 atacgcgctg aagtactgcg tgccgacctt gccgtaattg cccttgaggt acgctgccgt
     5401 attgcccttg gtcgcgagcg acttgaacgc cttcgcggcg tcctggtagc ggttcgcgtc
     5461 gaggagcgcg agcgcgtacg agaactcgaa cgagagcggc tcgatgcctt tcagcacatc
     5521 ggtgtttctt accgcgcgct cgaagtccga tgcggcatcc gtctgcttgc cgttgcgcat
     5581 gttctgccag ccgcggcggt acagcgcgag tgcgaggttg cgcttggcgg ccgatccgat
     5641 ctcggggtcc tgcgctgacg cctgcacagc gagctcgagc ttgtcgacgg cgccttccag
     5701 atccttgtcc cacgtgagcg gcgcccactc ggtgtagatc tcggcgagcg cgagctgcgc
     5761 gcccgccttc ttcgcctcgc tctcggccgc cgcaaacgct gccgaggcct tcgccacatc
     5821 gggcttgttg aagcacatgt agctgcggcc gatcagccgc tgcgtcaaca ccacgtcgcg
     5881 acccccgacg ccgttgagct tctgcagcat ccggttcgcg ttctcgcagt cgccgcgctc
     5941 gatcgcgagg accgcgatgt tcgtcagtgt cgtggtgttc tgcggttcga gcttgagcgc
     6001 ctcgtcgagg taccccaggg cttccttgta gtttttgccc tgttcgaacg cttgctcgtt
     6061 catcgtcagt acgaggtagc gcgtgatctg gacgtcgccc gcgcgctctt ggcgcgcggc
     6121 ctcgaagcgc gcacgcgctt ccttgagctt cttctggttg tagagcgcct ggccggagat
     6181 cacgaagagg gcgcccttct catcactggg cgccttcgcg aactcgggcg tcgcgacaac
     6241 cttgtcggtc aacgccatcg ccttgctgtc ctgcttgccg gccaggtacg cggtaccgag
     6301 ctcggccgcg agctcgaggc tgcccgggtt cgccgcccac gcgttgttca gcttctcgat
     6361 cgcgagcgcg aggttcgggt tggggccgct cgcaggtgcc ggcagacggc gatacgtttt
     6421 gccgaggcga atcgacagta agacctgctc gcgcgtctgg ttcggccgga gcagcttctc
     6481 tgcgcgcagg tactgatcca gcgcgttatt ccaatccttt tcttcgaaga acgtgtcacc
     6541 gatcagcatg aagccgcgcg cttcgttctt gcgcaggcgc gtgaactcgt taccggccgc
     6601 gcgcgccttc ttcatctgct tacgtgcgag gtaggcgttc gccaggttgt accaggctga
     6661 cccgttcgca tcgatgcggc gcggatcctg gatcacgcgc tcgcagacgc tgatcgcctg
     6721 gtcccaccgg ttgaggcccg agtacacggc acacaggccg ttgtcggcgt tggtctgcgc
     6781 accgggtttc ttgccgagct tgcgctggac gtactcgaac tgctccatcg ccttcgtgta
     6841 gagctgcttc gcgcgctgct cgtcgccgtc gttgagcacc gacttcgagt tcgagagata
     6901 cgcaaagccg agccggatgt gcggcagcac gctgtacttg ccagtacgcg agctgtcgcg
     6961 ctcttcgacc tcgatcgggc gggactgcag gtaggcttcg aacgcggccg cggtcttcac
     7021 cgagtcgcgg ccggcgtagt actcgccgat gatctgctgg ttcgtgaacg aggatggctt
     7081 cgacttcacg accctctcgg cgaccttgat cgcatcgtcc cggcgatcgg tctcccagag
     7141 catgagcgct tcctgctcga gcacctcggg ggcgttcggg tattggcgct tggcccgttg
     7201 ttcgatgaag tcgaggccgc ccttgaagtc cttgaggatg atgaagaggc cggcgcgctt
     7261 gccgtaggcc tcggcggaga cctgagcggg cgcactgttg atggcacgat cgtacgaggc
     7321 ggcggccttc ttgtagtcac cgtcttcgta gtacttgtcg ccttcgcgca agtcgtcttc
     7381 gacggatgcc atcgcgtact gcccgacgat catgatcgtc gctgcgacga ataaagccct
     7441 caatggatcc tcccgacgac catggtgatg tcgtctttgc gaatcgcttc gccgaagaac
     7501 gctccggcat cggcaatgat ggcgtcgcgc atctcaccgg cgtcgagtgc cgcagctttg
     7561 cggactgacg cgcggaagcg cttctcgccg tactcctcac ccgcctcgtt ttcacactcg
     7621 acgatgccgt ccgtgtacca gacgagcaca tcgccgatcg cgcactcagt ggtcttcgcc
     7681 tcgtacttcg agttgcgatc gtcgccgagg cggttaccgc gaatcatcag cgagccgaac
     7741 tcgcctttgc cctcaccggg gcggaacaga tacgggaagt tgtggccggc gttcgcgtac
     7801 gtgatcgtgc gtgccttcgt gtcgatgatc gacgcgaagc acgtcatcac gaaccggcgc
     7861 tgcgcgctct cgaagatcgc gtgattcatg atctcgagca gcgtggtcac ggtgacgtcg
     7921 tcgttgtgca cgtaacgtgc gacgtcacac gatgcctttg cggcagcggt gatcatcgcg
     7981 gacggcacgc cgtggccggt gacgtcgccg atgacgacca ggatcttgcc gccgacgagc
     8041 tcggcccacg tccaccagtc accgcccgtc tgtgcggccg gctggtagaa gcccgcgaac
     8101 gtcagcgtgc tcttcttcac cggtgcgtcg ctcggcacca gcgtttcctg aatcgctttg
     8161 gcgacctcga gctcttgctc gatcttcgct ttctcggctg tttgctgcag caggatcgcg
     8221 atctggtcgg ccatgaagtt gaagttctcg ccgagcatgc cgatctcgtc gcccgtgttc
     8281 acgtcgacgc gagcatcgag gtcgccgcgg gcgatctggt ccgccttcca cgcgagctgc
     8341 ttgatcggct tggagatcga gaggccctgg aagatcgcga gcacggtgcc gatcagcgcg
     8401 aacagcgcac cgacggcgcc ggtgtagagc gcggccttgg tgaccgcggc ggccttctgc
     8461 tcgtttgctt cggctttgaa ccactcgacc ggtgcgaggt cgtaaccgag cacgacgtag
     8521 ccctggcgcg agatcggagg gtcggcggcg atggttccag cagcgctggc cggcgggtcg
     8581 ccggaaaaga tcgggtacgc aaagatcttg tagttcgagt tgccggaggc gaggtcgaac
     8641 tcgacgagca cgttgcccgc cttggcgggg tcggcggcgt tcgccttcca tgccgcgagc
     8701 gccttgttcc agctctcgac ggtgatctgg ccaagcgcgg ggctcaccgg cgcgtgctcg
     8761 tcgaacacgt tgcagtccgc ggtcgagccg gtgccctcgt ggcagtgcgc gatgaccttc
     8821 tggttcagat cgagcacgta cgcgagcttg aggacgtagt ccttcttgcc gttgctctcg
     8881 cgggtgtcgc cggcgacggc gttcttcacg agcgtcagga tgtctttatc gcgacctttc
     8941 tcgagaacca gcgcctcggt cgcgcgcgcg aacagcggcg taccgatctg gccgaactgc
     9001 tgtcgaagtg actggaacag ctcgacgcgt ctttccgccg attcatcaaa ggctcggcga
     9061 aggttcatga cgttgagcaa acctgcgcca acgcccgtaa ccacgatcaa cagcgtggtc
     9121 gtgaggatca tcttgaccga gatgctcggc ccaccccgtt tggccatgta acgggagagt
     9181 atgccaggga cggccggact gctaccattg tgcccggatg gacaatacgt cacctgcgct
     9241 tacgcagttc cttaccgagt tcaagcgggt gaacaacgca atcgtcgata gctacttcat
     9301 cgtcgataca gagcgccgga ttgttgattt caaccgcgcg ttttatgcgc tcctgccccg
     9361 tagcgtcgcc cgcggcctca agggcaagca ctgttacgag gtgttggagc tgaacatttg
     9421 tgccaacgag tgcattgctc agcagtgctg ggctgacaac cggcacgttc gcctcgacga
     9481 aatctcggga aacatcgcag gcgaggctca aaagctgcgc ttcatcctgt ctgcggtccc
     9541 gatcaccgac gaggccggcg cccatgtcgg cgcgctcgaa atccagcgca acgtcaccga
     9601 cgaagcggtc gtccaggtca agtaccaaga gatgctcgaa aaggaggccc gcgagcgcga
     9661 gcgcttggcc aaccaggtcc gcatgcggac gaaggagctc ctcgagacga accagctcct
     9721 actcaagacc caaaaggagc tactcgccta caagaagggc ctgacggtct aacgtctcgc
     9781 ccgcgcaggg ctagtgatac tctgacggcc gtgtcgccgt tagattggcg ggcggtcggg
     9841 ttggtgctgg ttgcggcctg ctacagcgcc tcgccggcat tgaacgtgcc ctgcgggccg
     9901 aacggcgagt gcccgagcgg ccagcaatgt gacaccgccc agacgccgcc gacctgcgtg
     9961 ctcacactta tggatgcgtc ggtggcggtg gaggacgggc cgccgccgat tgatgcgccg
    10021 cccctcgaat gcacggtctc gaaagactgc accgacgctg gtacgccggt gtgcgatgac
    10081 agccagcacg tctgccgggc ctgcgtcgcc gacgccgaat gcgatggcgt ctgcaccgag
    10141 tacgacggcc agtgcgtcgg caacggccag gcgatcttcg tctcgccgac gggcaccgac
    10201 ggcggcaact gcatggcgag cgcgccgtgc cgtacgttca gctacgcatt tcagcagctg
    10261 acggcgaatc gtcgcgtgat tcgcgttggc gacggcatgt acatgcgcac cggcaacccg
    10321 gtgctggacg tgaccgacac cggcggccgt atcgtgttct cgggcgagga ccgcgatccg
    10381 gccggcgcgg tcatgaccgc ggttgcgaac agcacgacga ctccgccgat cgtcagcacg
    10441 gatcctggca ccgacgtcat cgtcgaaggc atcacgctca cgaacgccga taccgacgcg
    10501 gtccggtcga gtggaacgtt cttggtctcg cgtgtcgcga tcacgaacaa cggagatcgc
    10561 gggatccagc atcagccaac cagctctgcc gccgagctcc acgtctggga ctcgctcatc
    10621 gcaggcaaca gtggcgacgg cgtctatgca gaccgagggt cagcggaagt gctgcgctcg
    10681 accgtccaca acaacagcga cggtggcatg accttcgacg gcctgacctc ggtgaaagtc
    10741 gtcggctcga tcatcacgcg aaacggcaac ggggcctcga gctacggcgg gatcagaacg
    10801 atcaacgtcg gtgttcagga cgccgtcatc gcgttcgaca cgatcgcgtt caaccgctgc
    10861 tctgggacgg cagccgcgcc tggcctctcg acagccgacg tcatcaacgt caccaactcg
    10921 atcgcgaccg acaacaccaa tgacgcgaca gtgccccggc aaatctgtga cacgtgcacg
    10981 tcgagctatt cgctgttctc ggggaacgtg ccgcccggta ccggcaacat cgccggcccg
    11041 gcgcaattcg tcgacgcagt cggcaacaac ttccatctca tggcgacgtc gcccgcgcgc
    11101 gatgcagccg atccgggcgc gatgccggga catgatatcg acggcaattc gcggccgctc
    11161 ggtgctggat tcgacatcgg cgccgacgaa ataatgccgt aatgcgtttg cttgctctgc
    11221 tcgcggtcgc ggcctgcgcg cacacgccga acttgccgct gcgtccggat ccgtcgcccc
    11281 cagcaggctc gacgaccgaa acgatcacag cccgcgatgg cacgcagctg ctcgcgcgtc
    11341 attgggccgc cgtcggcgcc gggcagcccg gccccgacgg atctgtggca cggggaggag
    11401 acgcgcgcgg cgtggtcgtg atcatgcacg ggctcaagga ctactcggcg cgttacgcgt
    11461 cgctcgcgtc gcggctcgca gcaggcggct actcggttta cgcgttcgat ctgcgcggtc
    11521 acggtcggtc ggcgggcccg cgcgtcgcgc ccgatgcgtg gaccgactat gtcgacgatc
    11581 tcgatcgctt cctcacgacc gtcgagcagc gcgagccggg caagccgatc tttctgttcg
    11641 gccactcgat gggcggcgcg atcgcggcgc gcactgccga gctgcaccaa cccgcgctcg
    11701 ccggtctcgt gctgtcaggc ccggcactcg cgatcgacgc agcgccgctg ctgatctcgg
    11761 ccacgcgcct gatcgcggtc atgaacccgc gcgcaccggc gctgaagctg ccgaacggcg
    11821 atttctcgtc ggaccctgcg gcagccaccg cgatggacaa ggacgagctg gtctcgcaat
    11881 ccccggggcc ggcgcgcacg gcagctgggc tcgtcgacgg catgcgcgcg atctgggccg
    11941 acgtcgatcg gctgacgatg ccgctgctcg cgctgcacgg caccgccgat cgcctcaccg
    12001 caccctcggg cagccgcgct ctcgtcgagc acgcgccggc gcaagacaag acgctgcgca
    12061 tctacgccgg cttcttccac gatcttctcc acgagccgaa acgcgatcag gtcgaggcag
    12121 acattcatgc ctggctcgac gcccacaccg gcggagctgc cgttgcggcg ccgccgatct
    12181 acgccgagca tctgaatggc gatccgcgcg gctggacgca ggcgatcgaa ttcgccggcg
    12241 gcatctcgcg cggcttcgac gagaaagtga cgcgcttcgg cggtgagctc gcaatcaatc
    12301 tcgcgcggcc gcggccgttc ggctggcatg gcgcattcac ggcgcggctc gcaaacacgc
    12361 gctacgcggt cgcgctcaag ccgctcggcg ttgcggcccg tttcggaggc gccgtgttcg
    12421 gcctgtccgg cggcggcgcg ctgctcaccg acgcacacgt cggcgtcgca gcgggcggct
    12481 ggtacgagca gccggcgggt ccgctccacc tcggcatccg cgccgactac gagcatgcgt
    12541 tcttcgcggg tgcgcgcaac gtcggcttcc tcgtcggctc gctgcgcttc ggcggcgatc
    12601 gtgcgtactg gccgcacgcg cgtgcgggcg tcggaccgat cgtgagcggc ggctacgagt
    12661 gccagggcga tacgtgcggc ttcacggcga tgctcggtct gcagctctac ggcgctgact
    12721 aaccgccaag acgccgcaga cgccccagac gcagagacga gaggactaac cgccaagacg
    12781 ccggggacgc caagatcaga acaatcaatc gatcgcgctc cgctcgcaca cacgtccgat
    12841 cgaacaacta ccaacaacaa cgcgttgctc tctggtccga tcccaaagcc tctgggcttg
    12901 gcgtctgcgg cgtcttggcg gttgattctg cggtctagcc gacgcgccag atcttctgag
    12961 cgtactcgcg aaccgagcgg tccgacgaga aatagcccgc gcgcgcgaca ttgaggatcg
    13021 acgagcgcgt ccagcgcgcc gggtcgcgcc acgcggcggc aacggcgcgg tgtgcatcgg
    13081 cgtacgcgcg gaagtcggcg agcacgagga acggatcgcg ctcgacaaga tcggagacga
    13141 gctgcgcgta cgtgccgcga tcgccgcgcg agaacaggcc gctgctgatc agctcgatcg
    13201 cgttcgcgag ctcgttgtca tggcgaagca catcgatggg ccgatagccc tcgcgcttct
    13261 tcttgatcac ttcgtcggcg gtgaggccga acaaaaagaa gttttcggcg ccgacggcat
    13321 cgcgaatctc gatgttcgcg ccatcgagcg tgccgatcgt gagcgcaccg ttgagcgtga
    13381 acttcatgtt gccggtaccc gacgcctcca tgcctgccgt cgagatttgc tcggagagat
    13441 cggcggcggg gtagatgcgc tgcgccttct tcacgctgaa gtcgggcacg aacacgatgc
    13501 gcaggcggtc gcgggtgcgc ggatcgttgg cgacgacctc ggcgatgccg tgcgcgagcc
    13561 ggatgatcag cttcgcggcg cggtacgcgg gcgcggcttt gcccgcgagc acgatcgtgc
    13621 gcggcacgtc gctgtcgtcg ccgacgcata ggcgatcgaa gcacacgatc gcgtgcagga
    13681 gattcaagtg ctggcgcttg tactcgtgga tgcgcttgca ctgcgcgtca catagcgcgg
    13741 ctggatcgaa cgcgccgccg acgccaccga ggtgatccga taggtcgcgc ttgttcgcga
    13801 gcttgaccgc acgccagcgc tcctggaacg cggcatcgtc ggcgaaccgc tcgagctcgc
    13861 ggagccgctc gaggtcgcgc agccagtcgt cgccgatcgc gtcgttgatc acgtcggcga
    13921 ggcgcgggtt cgcgagcgcg acgaagcgcc gcggcgtcac accgtttgtc acgttcgtga
    13981 accgctcggg ccacatgtcg gcgaagtcgc gcagcacggt ttcgcgcagc aggcgcgagt
    14041 gcagctcggc aacgccgttg atgtgatggc tcgccacggt cgcgaggtac gccatgcgga
    14101 cttgtttctc gccgtcttcg ccgatgagag acatgcggcg gatgcgcgcg tcatcaccgg
    14161 ggaacttggc gctaacctcg tcgaggaagc gacgattgat ctcgtagatg atctcgagat
    14221 ggcgcggcag cagacgctgc acgagtggca gtggccacgt ctcgagcgcc tcgggcagca
    14281 gcgtgtggtt cgtgtacgcg aacgacttgc tcgtgatcgc ccacgccgca tcccagccga
    14341 gaccgtgcac gtccatgagc aggcgcatca gctcgggaat cgcgagcgtc ggatgcgtgt
    14401 cgttgagctg gatcgcgatc aggtcggcga gctgatgaat tgcggcgccg ccgatcgtga
    14461 gcaggcgaat gcagtcttgc agcgcgcacg agacgaagaa gtactgctgc tcgagccgga
    14521 gctgcttacc ggccgggctc gtgtcgttcg ggtagacgac cttcgtgagg ttctcgctgc
    14581 ggagcttggc ctcgaccgcg cgccagtact cgccgatctg gaacgcgtcg agatcgaact
    14641 cctcggcggc gaccgcgctc cacaggcgga ggaagtttgt cgtcggtgtg ccgtagccgg
    14701 cgaccgggac gtcgtagggg acgcctttga cgatgcgctc ggggtgccac gtcgcgccgg
    14761 tttcgccgtg ctcggtgtag ccgccgaacc cgaccgagtg ctcgacgtcg tagcggcgga
    14821 tctcccacgg gaagccgtgg cgcagccagc gatcggtgtg ctcgacctgc cagccgtcgc
    14881 ggatgtcttg atcgaagatg ccgaactcgt agcgcaggcc gtgaccgaca gccgcgatgc
    14941 cgagcgtcgc gagcgagtcc atgtagcagg cggcgagccg gccgaggcca ccgttgccga
    15001 ggcccggttc ttcctcgtgc tcgagcaaat cgtcgagcga gagcccgact tccttcgtcg
    15061 cctcgcgcac ggtctcctcg aggtcgagcg ccatcaggtt cgaaccgagc tgcgggccga
    15121 tcaggtactc cgcggagaga tagatcaccg tgcgctgctg gttcgtgagc accgcgcgtg
    15181 cggtatcgac ccagcgatgc agcaagcgat cgcgaacggc gtacgcgagc gcgagatagt
    15241 gatcgttgcg cgtggcgacg cctggaaact tcgcgagctc gacatacagc ttctcgagca
    15301 ggtcgagcgc aaagctgcgc gcgtcatcta ccatcagggc tatttgagct tggcgtatgc
    15361 cgccttgtcg aacccgacga tgactttctt gccggcgatg tagatcggcc gcttcaggag
    15421 gttcggctcc ttggcgaccg ccttcgcgaa cgtgtcggca tcgggagggc tttcgaccca
    15481 gcccttctgt ttcacgacgt cgttgcgggc gttgaccacc gcggcgaccg acccgaccgc
    15541 cttgatgatc gctttcaccg tttcgacatc gagcggcttc ttcgcgaaat cgactgcatc
    15601 gaactcgacc ccatcgttgc gcaaggaact cctcgcgtca cgacaggagg ttcaagtgct
    15661 cttccagtac agcgtgccct tcatgcgggc atcgtacatc gctcagcggt tcgcgcgcag
    15721 ccgtttgagc gtcgtgagca gctgctcccg ggtgatcaga tcgatcacgt cgagtgcgcc
    15781 atcctcgacg cggaacagca cgcggtgatg aatgccgacg cgcgccatga gcaccggccg
    15841 gaccatgtcc ttcgcctgtt tgacgctgcg ccacgcgaag ccatcgcccg cggcgagcgt
    15901 gccgaccgtg cgcatcgtct ccgaggcgac ggccgctggc acgtcggcga gtgcgtcgat
    15961 cgcgcggcgc tcgaagcgcg gaatcgcgat gccgcgtgcg ccgagctcga catcgtcgcc
    16021 gacgtcgtcg tcactctctt cgagcgtgac ccggcgggcg cgtggccctt cgcgtcgcgt
    16081 actcgcatct tcgctggccg cggactcgat ctgcttgcgg aggtcgcggc gctcggcctg
    16141 gccttcgcgg atcagcgcct ccagctcggc gacgcgctgg tcgagcacgc cgcgctgctc
    16201 gggtgcgcgc atcagctcgg cggcggggcg cgtgcgtgcg ctggcgagct cggtgcgcac
    16261 ggccgcgagt gctcgttcga gctcgttgat gcgcgccgac gattcggcca gcgactcgcg
    16321 cagcttcgcg tcgtcctccg tcggcttctt cgtggtcttg tcggtgagtg cttcgaggac
    16381 gtcccacgcc ggatcgtcgg gccgcacgtt cagccgatcg cgcgcgtcct cgacgagctc
    16441 cagcatcaga tccggatcgt cgacgttcag cgtgccgata caggcccggg ccgcgatgac
    16501 acccagggcg ggctcggcgc gcaacagcga gtacgcgagg tcgatcgcgg cagtcttctc
    16561 ggtgcatgcc gcgcgtgcgg cgtcgacgag cgccggccac gcatgctgcg ggccctcgga
    16621 aatcctgagc tcgagctcga acagcttcgc gaggtcagca ggcagctgcg cgatgtggtc
    16681 gcgg
//
LOCUS       DASUFX010000194        12425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_724394,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000194 DASUFX010000000
VERSION     DASUFX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724394"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFV99_07695"
     CDS             complement(<1..1252)
                     /locus_tag="VFV99_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5059226.1"
                     /translation="MADSAPTAEELLAHTEWLTRLARALVGDVAAGDVVQDTYEAALA
                     KPSNRRGPLRPWLGGVARNMARMAARGRARRERREQAVPVHDDVPSPEQLLARAQIQQ
                     KVNRLVLELHEPLRSTLLLRFFEGLSAAEIARAQNIPAATVRSRLKDALDRIRAELDA
                     EHGNDRRAWVGLLAPHLPAPAAMSHGTAASAGGLIVSTQVKVLIAVVVAALIVVGTRV
                     AGLWGSGAAEKPTPVANATPPSNAPVKSAQTAAPTASARELPTIHDDDPKGTLRLEGQ
                     VIDEHDAPVAHAIVAIDANPPIFVETESDGAFVFEGLLPRDYHIEATAADLYAGPASL
                     RLSYKLEPVTLRMRKGGTVEVTVTERATGAPVKGAEVELRSTISTLTWKATTNTDGAV
                     RLTGLGAGSSPLVARAKGYARATMW"
     gene            1270..1884
                     /locus_tag="VFV99_07700"
     CDS             1270..1884
                     /locus_tag="VFV99_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059227.1"
                     /translation="MPGKVLPGFAQFPQMARGWSKFWQQFAARRQRVGMRHPMSRLDY
                     PCSFALILAIAACGGSSPEPAKPSPQPAARDTADLVPMCKRIFARKATCADDYLPVLL
                     DLRVELNIPSGIGDEVKSQGRDAVLAIAHTELARDTETANVDAVCETAAAQASKAPPE
                     RRDQLLEQGARCEAATDCKGFAVCVVEIDRSFIVAGAQRRAAGS"
     gene            complement(1942..5148)
                     /locus_tag="VFV99_07705"
     CDS             complement(1942..5148)
                     /locus_tag="VFV99_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEX5059228.1"
                     /translation="MGKSSDQPRADRGFVLRTPLLPLETFACWQSASDPRAFLAEIVS
                     RPEVREALFVATPSLSEGIEKWRTAGDSPSGQQVELSLARYVARMAGRSTPFGLFSAV
                     AAGQLATDTSLELAPIADYRRRTRLDNDYLFALIDEICKQPAARSRLRYRPNSSLYTV
                     AGRLRYAEARLLDKERSYHLVSIDPTPYLAATLERARSGARLAHLAAGLAEADPDVTL
                     EEAIAYVDELVAAQVLVPELGITVTGSEPIDGLIAQLTAAELDEPARVLCDARDAIAR
                     IDERGVGNDTARYHAIAAQLRQLPAPVELARLFQVDAVKPATAVVGTKLVAEVARTIE
                     WLRKIQKPLRQPTLDQFRTAFAERYESRVVPLVEVLDEESGIGFEAVSGPGSEGSPLL
                     AGMRFPGSPGAEQTAWGAFERHMQRRLIAALDTGANEIVLGDGDLEALQTPQPPAAPD
                     AYHVEIRLQRGTGSAPTILIESASGPSGARMLGRFCHASSEIDQIVREHLRAEEAQRP
                     DAVFAEVVHLNEGRIGNIVCRPVLRDHEIPFLGLSGASGAHQIPVDDLLVSVRGNRIV
                     LRSRKLGREVIPRLTTAHNFRLRSLGIYRFLCALQDSCAISWSWSSLGGHPYLPRVRI
                     GNVVVARATWNLNASDLEPLTQTVREMNNKRAHTIPLADRRARIADAVTELRTRLRLP
                     RFLVIADYDNELPIDLDNPLLAHVFADELSGQSQATLYEQFPTPDASPVRGPEGTFAN
                     ELVLLFVRPPPALAAAAPDEPIARNARTPPPARVQRSYPPGSSWLYAKLYTGASTTDR
                     LLREAIGPVARGAIEHRDATHWFFVRYGDPHNHVRVRFAGDPARLTSRVLPALADAIE
                     PFRVNGAVWKIQLDSYDREIERYGGDRGIELVEKLFWIDSEAVLGIVELLDGDAGADA
                     RWRLAVRGSESLLAALGLDPDTRYRIFSDARDSLGREFRAGADFYAPIGERFKAERAA
                     LEALFAPDPARDAKHDLAPGLELIARRDQRLEPIALELRARDAAGELAPKLGDIAWSL
                     VHMHVNRLLHASQRAQELVLYDLLRRLHEGRRARAKTA"
     gene            complement(5169..6050)
                     /locus_tag="VFV99_07710"
     CDS             complement(5169..6050)
                     /locus_tag="VFV99_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5059229.1"
                     /translation="MKRAAVALIYVLGCGGASDDPPSTDPMREPRVQQATRSETREDL
                     DKPGFLGVLMPRGLAEVISPFTSTVSKLEVRLGDRVTRGQRLAQLDERPLREELLIAN
                     ATLKASQASAAQADVERRAAASILEREKTAFEQGISSGAELSTAEFNKSKAEMNAARA
                     AATVQEQRARIAQLTARLGDTSLVAPIDGRVALMYVHEGDRVEEGRGVIRVISSDELF
                     VKFAIPSDKVGTVKPGDNVDISIGQLAITTTGVVRHVAPEIDPVAKMILADADLVSPP
                     ASLQSGIDCRIVPRTGP"
     gene            complement(6047..8275)
                     /locus_tag="VFV99_07715"
     CDS             complement(6047..8275)
                     /locus_tag="VFV99_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX5059230.1"
                     /translation="MTAPRRTDEVADLFPSLERLARKRHGRVPFVQQLEAADCGAASL
                     AMVLAYFGHDATLDEVREAVGVSGRDGTDAASIVRGAEWYGLRARGIRIDVEHLRFVP
                     AGTILHWELNHFVVFERVTRRGVAIVDPAMGPRVIPLAKIGESFTGIALVFEPADTFE
                     KKKRGTGRVAWYARQLAGQRHVLGRVLVTSILLRVFALALPLVTAVIVDRVVPRADHS
                     LLLVVVMGIAGLLLFQLITTLVRSHLLLQLRTNLDTHLTLGFVDYLSRLPFQFFQRRS
                     AGDLLMRVNNNATIRETLTSSSLSAIMDGTLVIGYAVLVFAFAPLMGALVVMLGALQI
                     SVYTFARRSYREMMARSLEAQARQQSYLVEMLGGMETLKAAAAEARAVERWSNLYIDE
                     LNVSLDRGRLAARIDAATGVLGLASPLAVLVVGALQVMSGVLTLGEMLAIIALAVGVL
                     TPLSALVNSALQLQLLGGYMGRIDDVLRNDPEQLGKDVARAPKLSGRVTLQHVSFRYG
                     DNTPYVVRDVSLDIRAGMTVAIVGRSGCGKSTLARLLAGLYQPTDGRILFDGHDLARL
                     ELKSVRRQIGMCSQAPSLFAGSIRSAIALSMPMAPMERVLDAARRAAIHEDITAMPMA
                     YDTVLADGGQSLSGGQRQRIALARALVHDPAILILDEATSALDSETERTVIKNLEQLR
                     CTRIVLAHRLSTIVNADLILVMDAGEVVESGTHQELVSRSQHYSRLVAAQLQPESHRG
                     VA"
     gene            complement(8272..10155)
                     /locus_tag="VFV99_07720"
     CDS             complement(8272..10155)
                     /locus_tag="VFV99_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5059231.1"
                     /translation="MPVGSSAITVVGFGRIGAGPRVLVADGDAASRQWLRSALAGDLA
                     IDEIDRGDLALDLIAAGKPRLVVIGAQLVDMSGSELIERAAQWFDDRHARMGVFLLTD
                     AAGNVADVDESRVSVFYRLTPAMQPARARELMKQAASTLPPTPPQDEAVPPHVAPHVE
                     RIGAQDDPASAAQAAIGAVIALLGATRARCLYCDEDTGLLWPEGEDGEHEAKASAGLV
                     GFAARSGACIVVPSAADDALYRADIDDPTGDGRERLAVQPVVGLDGHVHAVLIAIRTA
                     GQPPFTDEDSATLRALATGWAPHLQQLAMRAEADNILGDRLDAGPSELFRQEAIVSLV
                     RRGARGDVVRVHPGWIRAAYWIVLASLLGMIAFASLAKVHEYTEGPAIVRATGRNEVV
                     AMEPGTITALEVQRGAIVKAGQPLARLHDAEQLGRLRGLETEFERKLVAYLQTPADTS
                     VRQALAQIVSQRESARAGVEAKMIRAPRDGIVKEVLVRNGQRVDAGATVMSIIEQGAT
                     EGLSLYAFVPGSQRPRLRVNQRLDLTLPGYRGARITTRVLAVSSEVLGADDARMRYLG
                     ERVSASLPVQGTVVVVEAQLASPTFEADGEHYQLHDGMIGTAEIRLASRSVLETMIPG
                     LSQ"
     gene            complement(10210..>12425)
                     /locus_tag="VFV99_07725"
     CDS             complement(10210..>12425)
                     /locus_tag="VFV99_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5059232.1"
                     /translation="TEPVVLKLRAAAAVDVTVVDGERGQPIAEARVEVRAPIVTSANT
                     ARDGAATLSPIIPGRWPLVVSAPGFATAFEAVTASPTAHSKVAVALTRGFHARGRVID
                     RAEKPVDGALVWLESASDWVSTSDPSRDGVRSSADGSFDVAGVARGSYVVSVIAPNRA
                     TATSAVVHVDHDVDGIVVRIVSDQQLAGRAVHRDRTPAPGAAVRVWWADGARVSHVDA
                     TGAFVIDGMPRSEIWISASDTDASSKAQRIDLSMGAPKELELVLENTASIAGMVVDQT
                     GNPVEGAQVTGERKDVVDGNAATFRQGLSDSSGRFELRGLVAGHYQLSAVREPMSGAA
                     GARVFVQTGQGDVRLVLESNGRIRGRVAFETGGAPAAYTVRVGRAGLPTAFTNESFEI
                     ESAPGARSLWIEGPGFTARSVDGLEVKADDVTDTGTIKLDRGRTLRGRVSAAQGVSVA
                     NAEVIAGALLSGTGQRVDSGDNGPSFRSDVKRTPVRPDGSFELTGVATTGVSVVATLD
                     SNARSAPVAVPPGTADVDGIVLTVAREARLSGTVSRAGKPTRAIVNAQSYESPLAAST
                     VMTQDGSYSFDRLAPGRYSVAAIAGAPLAGSPFYPRSVELAPSSAQTLDLTIAPGDST
                     LTVRAPGIAAGLVFVTTRPGTATSALALLTELGRQDGGQWAMTPVNDQTASFRELAHG
                     PARVCVVQLTGKAAQASALVEQLARTGASLTASCVTTELGATTTVTIGGAANPTK"
BASE COUNT         1984 a         4134 c         4301 g         2006 t
ORIGIN      
        1 gccacatcgt ggcccgcgcg taacctttgg cgcgtgcgac gagcggcgac gagccggcgc
       61 cgaggccggt gagcctcacc gccccatcgg tgttggtggt cgccttccag gtcagcgtgg
      121 agatcgtgga gcgcagctcg acctcggcgc ctttgaccgg tgcgccggtg gcgcgctccg
      181 tgacggtcac ctcgacggtg ccgcccttgc gcatgcgcag cgtgaccggc tcgagcttgt
      241 atgacagccg caggctcgcg ggaccggcgt agagatcagc cgcggtcgcc tcgatgtggt
      301 agtcgcgagg gagcaggccc tcgaacacga acgcgccgtc gctctcggtc tcgacgaaga
      361 tcggcggatt cgcgtcgatc gcgacgatcg cgtgcgcgac cggcgcatcg tgctcgtcga
      421 tcacctggcc ctcgaggcgc aacgtgcctt tcggatcgtc gtcatgaatc gtcggtaact
      481 cgcgcgcgct cgcggtgggc gcggcagtct gcgcggactt gaccggcgca ttcgacggcg
      541 gtgtggcgtt cgccaccggc gttggtttct ccgcggcgcc gctgccccaa agtcccgcga
      601 cgcgcgtacc gacgacgatg agcgcggcga cgacgactgc gatcaatacc ttgacctgcg
      661 tgctcacgat caaacctcct gcggatgcag cggtgccatg cgacatggcc gccggcgccg
      721 gcaggtgggg ggcgagcaaa cccacccacg cgcggcgatc gttgccgtgc tctgcgtcga
      781 gctcggcgcg aattcgatcg agcgcgtcct tcagccgcga tcgcacggtc gcggcaggga
      841 tgttttgcgc acgcgcgatc tccgctgccg acagcccttc gaagaagcgc aacagcagcg
      901 tcgagcgcag tggctcgtgg agctcgagga caaggcgatt gaccttctgc tggatctgcg
      961 cgcgcgcgag cagttgctcg ggcgatggca cgtcgtcgtg caccggcacc gcttgctcgc
     1021 gacgctcgcg gcgcgcacga ccgcgggcgg ccatgcgggc catgttgcgc gcgacaccgc
     1081 cgagccacgg acgcagcgga ccgcggcggt tcgatggctt cgcgagcgcg gcctcgtacg
     1141 tgtcctgcac gacatcaccg gccgcaacgt cgccgacgag cgcgcgggcc aggcgcgtca
     1201 gccattcggt gtgtgcgagc agctcttctg ccgtcggtgc ggaatcggcc atgcgctacc
     1261 ttgttgccga tgccgggcaa agtgttgccc ggtttcgcgc aatttccgca gatggctcgt
     1321 ggctggtcga aattctggca acagtttgcg gcacggcggc aacgggtggg catgaggcac
     1381 cccatgtccc gactcgacta cccttgctcg ttcgccctga tcctggccat cgccgcatgc
     1441 ggcggatcgt cgccagaacc cgcgaagccg tcgccgcagc cggccgcgcg tgacacggct
     1501 gatctcgttc cgatgtgcaa acgcattttc gcgcgcaaag cgacgtgcgc cgatgattat
     1561 ctgcctgtgc tactggacct gcgcgtcgag ctgaacatcc cgtccggcat cggcgacgag
     1621 gtaaagtccc agggccgcga cgcagtgctt gcgatcgccc ataccgaatt agcgcgcgat
     1681 accgagacgg cgaatgttga cgctgtctgc gagacagctg ccgcgcaagc gtcgaaggcg
     1741 ccgcccgagc gacgagacca gttactcgaa caaggcgcca ggtgcgaagc ggccaccgac
     1801 tgcaaagggt tcgcggtgtg tgtggtcgag atcgatcgga gcttcatcgt cgcgggcgcg
     1861 caacggcgtg cagcgggctc ctagcctgcg ctaattgaca cacgtctgca gtcgccacgt
     1921 tcacgccgaa cagcgctggc cttacgccgt cttcgcccgg gcgcgacgac cttcgtgtag
     1981 ccgtcggaga aggtcgtaaa ggacaagctc ttgggcgcgt tgggacgcgt ggagcaggcg
     2041 gttgacgtgc atgtggacga ggctccacgc gatgtcaccc aacttcggcg cgagctcgcc
     2101 ggcggcatcg cgagcgcgca gctccagggc gatcggctct aggcgctggt cgcggcgggc
     2161 tatcagctcg aggccgggtg cgagatcgtg tttcgcgtcg cgtgcggggt cgggagcgaa
     2221 cagtgcttcg agcgcggcgc gctcggcctt gaagcgctcg ccgatcggcg catagaagtc
     2281 cgcgccggcg cgaaactcgc ggccgagaga atcgcgcgcg tcggaaaaga tgcggtagcg
     2341 cgtgtcggga tcgagaccga gcgcggcgag tagagactcg gagccgcgca ccgcgagccg
     2401 ccagcgcgcg tcggcgcccg catcgccgtc gagcagctcg acgatgccga gcacagcctc
     2461 actgtcgatc caaaacagct tctcgacgag ctcgatgccg cggtcgccgc cgtagcgctc
     2521 gatctctcga tcgtacgagt cgagctggat cttccagacc gcaccgttga cgcggaacgg
     2581 ctcgatggcg tcggcgagtg ccggcagcac gcggctcgtg aggcgcgccg ggtcgccggc
     2641 gaagcgcacg cgcacgtggt tgtggggatc tccgtagcga acgaagaacc agtgcgtggc
     2701 gtcgcgatgc tcgatcgcgc cgcgtgcgac cggaccgatc gcctcgcgga gcagtcggtc
     2761 tgttgtcgat gcgccggtgt agagcttcgc gtacagccag ctcgagcccg gcggatacga
     2821 gcgttgcacg cgcgcgggag gtggcgtgcg cgcgttgcgc gcgatcggtt cgtccggtgc
     2881 ggccgcggct agcgcgggcg gcgggcgcac gaagagcagc acgagctcgt tcgcgaacgt
     2941 accctcgggg ccgcggacgg gcgatgcatc gggcgtcggg aactgctcgt agagggtcgc
     3001 ctgcgactgg ccagacagct cgtcggcgaa cacgtgggcg agcagcgggt tgtcgagatc
     3061 gatcggcagc tcgttatcgt agtccgcgat gacgaggaag cgaggtaggc gaaggcgcgt
     3121 gcgcaactcg gtgaccgcgt cggcgatgcg agcgcggcgg tcagcgagcg ggatcgtgtg
     3181 cgcacgcttg ttgttcatct cgcgcacggt ctgcgtcagc ggctctagat ccgacgcgtt
     3241 caagttccac gtcgcgcgcg cgacgacgac attgccgatg cgtacgcgcg gcagataggg
     3301 atgaccgccg agcgaagacc acgaccagga aatggcgcag gaatcctgga gcgcgcacaa
     3361 gaagcggtag atgccgaggc tgcgcagccg gaagttgtgc gcggtggtaa gccgcggaat
     3421 gacctcccgg ccgagcttgc gcgagcgcag cacgatgcgg ttgccgcgca ccgacacgag
     3481 cagatcatcg acgggaatct ggtgcgcgcc ggaagcgccg gacaggccga ggaacggaat
     3541 ctcgtggtcg cggagcacgg gccggcacac gatgttgccg atgcgtcctt cgttgaggtg
     3601 gacgacctcg gcgaacacgg cgtcgggccg ctgtgcttcc tcggcgcgca ggtgctcgcg
     3661 cacgatttga tcgatctcgc tcgacgcgtg acagaaccgg ccgagcatgc gggcgcctga
     3721 cgggccgctg gccgattcga tcaggatcgt cggtgcgctg ccggtgccgc gctgcaatcg
     3781 aatctcgacg tgatacgcgt cgggggcggc aggcggctgc ggcgtttgta gcgcctcgag
     3841 gtcgccgtcg ccgagcacga tctcgttcgc gccggtgtcg agggccgcga tcaggcgccg
     3901 ctgcatgtgg cgctcgaacg cgccccacgc cgtttgctcg gcgcccggcg agcccggaaa
     3961 ccgcatgccg gcaagcagcg gcgagccctc cgagccggga ccgctcaccg cctcgaagcc
     4021 gatgcctgat tcctcgtcaa gcacctcgac gagcggcacg acgcgagact cgtagcgctc
     4081 cgcgaatgcc gtgcggaact gatcgagtgt cggttgtcgg agtggcttct ggatcttgcg
     4141 cagccactcg atcgtgcggg ctacttccgc gacgagcttc gtgccgacga cggcggtggc
     4201 cggcttgacc gcatcgacct ggaacagccg agcgagctcg accggagccg gtaactggcg
     4261 cagctgcgcg gcgatggcgt gatagcgggc cgtgtcgttg ccgacgccgc gctcgtcgat
     4321 gcgggcgatc gcgtcacgcg cgtcgcagag cacgcgcgcg ggctcgtcga gctcggccgc
     4381 cgtcagctgc gcgatgagcc cgtcgatcgg ctcgctgccg gtgaccgtga tgccgagctc
     4441 gggcacgagc acctgcgcgg cgaccagctc gtcgacgtac gcgattgctt cctccagcgt
     4501 cacgtccgga tcggcctcgg cgagcccggc ggcgaggtgc gcgagccttg caccgctgcg
     4561 cgcgcgctcg agcgtcgccg cgaggtacgg cgttggatcg atcgacacca ggtgatagct
     4621 gcgctctttg tcgaggagtc gcgcttctgc gtaacgcagc cgcccggcga ccgtgtagag
     4681 cgagctattc gggcgatagc gcaggcgcga gcgcgcagcc ggttgcttac agatctcgtc
     4741 gatcagcgcg aagagatagt cgttgtcgag gcgcgtgcgc cggcggtagt ccgcgatcgg
     4801 cgcgagctcg agcgacgtgt cggtggcgag ctgtcctgcc gctaccgcgg agaacagtcc
     4861 gaacggcgtg ctccgtcccg ccatccgtgc gacgtagcgc gcgagcgaga gctcgacctg
     4921 ctgaccgctc ggcgagtcac cagcggtgcg ccacttctcg atgccctcgg agaggctcgg
     4981 tgtcgcaaca aatagcgcct cgcgaacctc gggtcgcgac acgatctcgg caagaaacgc
     5041 acgtggatca ctcgcgctct gccagcacgc gaacgtctcc agcggaagca gcggtgttcg
     5101 cagcacgaag ccgcgatcgg cgcgcggctg gtcactcgat ttccccacgt ggcagatcct
     5161 ttgctggctc aaggccccgt gcgtggaacg atccggcagt cgatacccga ctgcagactc
     5221 gccggcggcg aaacgaggtc ggcatcggcg aggatcatct ttgcgactgg atcgatctcc
     5281 ggcgcgacgt gacgaacgac acccgtcgtc gtgatcgcga gctggccgat cgagatgtcc
     5341 acattgtcgc ccggtttcac cgtccctacc ttgtcggaag ggatcgcgaa cttgacgaac
     5401 agctcgtcgc tcgagatcac gcggatcacc cctcggcctt cctcgacgcg atcgccctcg
     5461 tggacgtaca tcagtgccac tcggccgtcg atcggtgcga cgaggctcgt atcgccgagc
     5521 cgcgcggtca gctgcgcgat gcgtgcgcgc tgctcctgca cggtcgcggc agcgcgcgcg
     5581 gcattcatct ccgctttgct cttgttgaac tctgctgtcg acaattcggc gcctgacgag
     5641 atgccctgct cgaacgcggt cttctcgcgc tcgaggatcg acgcggcggc gcggcgctcg
     5701 acatcggcct gcgcagcgga tgcctggctg gccttgagcg tcgcgttcgc gatcagcagc
     5761 tcttcgcgca gcggccgctc gtcgagctgc gcgagtcgct ggccgcgcgt gacacggtca
     5821 ccaagcctga cctcgagctt ggacaccgtg ctcgtgaacg gcgagatgac ctcggcgagg
     5881 ccgcgcggca tcagcacgcc gaggaatcca ggcttgtcga ggtcctcgcg cgtctcgctg
     5941 cgtgtggcct gctggacgcg tggctcgcgc atcggatcgg tggatggcgg atcgtcgctc
     6001 gcgcccccgc acccgagcac gtagatgagc gcgacggcgg cgcgcttcat gcgacacctc
     6061 ggtgcgactc tggctgcagt tgtgccgcga cgagacgact gtagtgctgg ctgcgcgaca
     6121 ccagctcttg gtgcgtgccc gactccacga cctcaccggc atccatcacg aggatcaggt
     6181 ctgcgttgac gatcgtgctg agtcggtgcg cgagcacgat ccgcgtgcag cgcagttgct
     6241 cgaggttctt gatcacggtg cgctcggtct cgctgtcgag cgcgctggtc gcttcgtcga
     6301 ggatgagaat cgcgggatcg tggacgagag cgcgcgcgag tgcgatgcgt tgacgctggc
     6361 caccagatag tgactggccg ccgtcggcga gcacggtgtc gtatgccatc ggcatcgcgg
     6421 tgatgtcttc gtggatcgcg gcgcggcgtg cggcgtcgag cacgcgctcc atcggggcca
     6481 tcggcatcga cagggcgatc gcgctgcgaa tcgaaccggc gaacagcgac ggcgcttgcg
     6541 agcacatgcc gatctgccgg cgcacagact tgagctcgag gcgtgcgagg tcgtggccgt
     6601 cgaacaggat gcggccgtcg gttggctgat agaggccggc gaggaggcgt gcgagcgtcg
     6661 acttgccaca acccgagcga ccgacgatcg cgacggtcat gccggcccgg atatccagcg
     6721 agacgtcgcg gacgacgtac ggcgtattgt cgccgtagcg aaacgacacg tgctgcagcg
     6781 tgacgcggcc gctgagcttc ggcgcgcgag ccacgtcttt gccgagttgc tcgggatcat
     6841 tcctcagaac gtcgtcgatt cgacccatgt agccgccgag cagctgcagc tgcagcgcac
     6901 tgttgacgag tgcagagagc ggcgtcagca caccaaccgc gagtgcaatg atcgcgagca
     6961 tctcgccgag tgtcagcacg ccggacatca cctggagggc gccgacgacg agcacggcga
     7021 gcggcgaagc cagcccgagc acacccgttg cggcatcgat gcgcgcggcg agtcgaccgc
     7081 ggtcgagcga aacgttgagc tcgtcgatgt agagattcga ccagcgctcg actgcgcgcg
     7141 cctcggcagc ggctgccttg agcgtctcca tgccgccgag catctcgacc aggtaagact
     7201 gctgacgcgc ctgcgcttcg agcgagcgcg ccatcatctc gcggtagctg cgtcgcgcga
     7261 atgtataaac cgagatctgg agcgcgccga gcatcacgac gagtgcaccc atgagcggtg
     7321 caaacgcgaa cacgagcacc gcgtacccga tgacgagcgt gccgtccatg atcgccgaca
     7381 gcgagctcga cgtcagcgtc tcgcgaatcg tcgcgttgtt gttcacccgc atgagcagat
     7441 cgccagctga tcggcgctgg aagaactgga atggcagcct cgacaagtag tcgacgaagc
     7501 caagtgtgag gtgcgtgtcg aggttcgtgc gcaactgcag cagcaggtgc gaccggacga
     7561 gcgtcgtgat cagctggaag aggaggaggc ccgcgatgcc catcacgaca acgagcagca
     7621 agctgtggtc tgcgcgcggg acgacgcgat cgacgatcac tgccgtgacg agcggtagcg
     7681 cgagcgcgaa tacgcgcagt aagatcgatg tgacgagcac gcgaccgagg acgtgacgct
     7741 gcccggcgag ctgccgtgcg taccaggcga ctcgccccgt gccgcgtttc ttcttctcga
     7801 acgtgtcggc gggctcgaac acgagtgcga tgccggtgaa cgattcgccg atcttcgcga
     7861 gcgggatgac ccgcggcccc atcgccggat cgacgatcgc gacaccgcgc cgagtcaccc
     7921 gctcgaacac gacgaagtgg ttgagctccc agtgcaggat cgttcctgcg gggacgaagc
     7981 gcagatgctc gacgtcgatg cggatgccgc gcgctcgcaa gccataccac tcggcgccgc
     8041 ggacgatcga ggcagcatcg gtgccatcgc gaccgctgac gccgaccgcc tcgcgaacct
     8101 cgtcgagcgt ggcgtcgtgg ccgaagtacg ccagcaccat cgcgagactc gccgcaccgc
     8161 aatcagcggc ctcgagctgc tgcacgaacg gcacgcgtcc gtgtcgcttg cgcgcgaggc
     8221 gctcgagcga cggaaatagg tcggcgactt cgtccgtacg tcggggcgcg gtcactgcga
     8281 caaccccggg atcatggttt ccagcacgct tcgcgacgcg agccggattt cggcggtgcc
     8341 gatcatgccg tcatgcagct ggtagtgctc gccatcggcc tcgaacgtcg gcgatgcgag
     8401 ttgggcctcg acgacaacga ccgtgccttg cacgggcaag ctggcgctca cgcgctcgcc
     8461 gaggtagcgc atacgcgcat catccgcgcc gagaacctct gagctgaccg cgagcacgcg
     8521 cgtcgtgata cgtgcgccgc ggtagccagg cagcgtgaga tcgagccgct ggttcacgcg
     8581 caggcgaggg cgctggctgc cgggcacgaa tgcatacagc gacaagccct cggtcgcgcc
     8641 ttgctcgatg atcgacatca cggtcgcgcc agcatcgacg cgctggccat tgcggacgag
     8701 cacctccttg acgatgccgt cgcgcggagc gcggatcatc ttcgcttcga caccggcacg
     8761 agcgctctcg cgctgcgaca cgatttgcgc gagcgcttgc cgcacgctcg tgtcggccgg
     8821 cgtctggaga tacgcgacga gcttgcgctc gaactcggtc tcgagcccgc gcagtcgacc
     8881 aagctgctcg gcgtcgtgaa gacgcgcgag cggctgaccc gctttgacga tcgctccgcg
     8941 ctgcacttcg agtgctgtga tcgtgcccgg ctccatcgcg acgacctcgt tgcgtcccgt
     9001 ggcgcgcacg atcgccggtc cctccgtgta ctcgtgaacc ttcgcgaggc tcgcgaacgc
     9061 gatcatgccg agcagcgacg cgagcacgat ccagtacgcg gcgcggatcc agcctggatg
     9121 cactcgcacg acatcgccgc gagccccgcg gcgcacgagg ctcacgatcg cctcctggcg
     9181 gaacagctcc gacggtccgg cgtcgagccg gtcgccgagg atgttgtccg cttcggcacg
     9241 catcgcgagt tgttggagat gaggcgccca tcccgtcgcg agcgcgcgca acgttgcgct
     9301 gtcctcgtcg gtgaacggcg gctggcctgc ggtacggatc gcgatcaata cggcgtggac
     9361 gtggccgtcg agcccgacaa ctggctggac tgcgagtcgt tcacgcccgt ccccggtcgg
     9421 gtcgtcgatg tcggctcgat acagtgcgtc gtcggcggcg ctgggcacga cgatgcatgc
     9481 tccgctacga gccgcgaatc cgacgaggcc cgccgacgct ttcgcctcgt gctcgccgtc
     9541 ttcaccctcc ggccacaaca ggccggtgtc ttcatcgcag tacaggcaac gagctcgcgt
     9601 cgcgccgagc agcgcgatga cggcgccgat cgccgcttgc gcggccgacg cgggatcgtc
     9661 ctgcgcgccg atccgttcga cgtgcggtgc gacgtgtggc ggcaccgctt cgtcctgggg
     9721 tggcgtgggt ggcaacgtgc tcgccgcttg cttcatcagc tctcgcgccc gcgccggctg
     9781 catcgccggc gtcaggcgat agaacacgga gactcgcgac tcgtcgacgt cggcgacgtt
     9841 gccagcggca tcggtgagca agaacacgcc cattcgtgcg tggcggtcgt cgaaccattg
     9901 cgcggcgcgc tcgatcagct cgctgccgga catgtcgaca agctgggcgc cgatgacgac
     9961 gaggcgcggc ttgcctgcag cgatcaggtc gagcgcgagg tcaccgcgat cgatctcgtc
    10021 gatcgcgaga tcgccggcaa gcgcactccg aagccattgc cggctcgccg catcgccatc
    10081 ggcgaccaac acgcgagggc ccgcaccgat tcgtccgaat ccgacgaccg tgatcgcgct
    10141 agagccaacc ggcatcacgg cgcggactct actgtgcccg caagcactga tcgacggcgg
    10201 aactccggtt cacttagtcg ggttcgcggc gccaccgatc gtcaccgtag tcgtcgcgcc
    10261 gagctcagtc gtgacgcacg atgccgtgag tgacgctccg gtgcgcgcga gctgttcgac
    10321 gagggcgctt gcctgtgctg cttttcccgt aagctggacg acgcatacgc gtgccggtcc
    10381 atgtgccagc tcacggaacg acgcggtttg gtcgttcacc ggcgtcatcg cccactggcc
    10441 accgtcttgg cggccgagct cggtgagcaa cgcgagcgct gacgtcgcgg tgcccggacg
    10501 tgtggtcacg aacacgagac cggccgcgat tcccggtgcg cgcacggtca gtgtcgagtc
    10561 accgggcgcg atcgtgaggt cgagcgtctg agcactgctc ggtgctagct ccaccgagcg
    10621 cggatagaac ggagagcctg cgagcggggc accggcgatc gcagccacgc tataacgacc
    10681 gggggcgagc cggtcgaagg aatagctgcc atcctgtgtc atcacggtcg atgcggcgag
    10741 tggagactcg taggattgcg cgttgacgat cgcccttgtc ggtttgccag cgcgagagac
    10801 ggtgcccgac agccgcgctt cgcgtgcgac ggtgagcacg attccgtcga cgtctgcggt
    10861 gccgggcggc acggcgacag gcgccgatcg cgcgttcgag tcgagcgttg cgaccaccga
    10921 cacgccggtc gtcgcgacac cggtcagctc gaagctgccg tcggggcgta cgggcgtgcg
    10981 cttcacgtcg ctgcgaaacg acggtccgtt gtcgccgcta tcgacgcgct gaccggtgcc
    11041 agacaacagg gcgccggcaa tgacctcggc gttcgcgacg ctaacgcctt gcgccgcgct
    11101 gacgcgtcct cgcagcgtac gaccgcgatc gagcttgatc gtgccggtgt cggtgacgtc
    11161 gtctgccttc acctcgaggc catcgacgct acgcgcggtg aagccggggc cttcgatcca
    11221 cagcgaccgt gcgcccggtg cggactcgat ctcgaaactc tcgttggtga aagccgtggg
    11281 caatccagct cggccgacgc ggacggtgta cgccgcgggc gcgccgccgg tctcgaacgc
    11341 gactcgtccg cggatacggc cattcgactc gagcacgagt ctcacgtcac cctgtccggt
    11401 ctgaacgaag acgcgagcac cggcggctcc gctcatcggc tcgcgcaccg cggagagctg
    11461 gtagtgaccg gcgacgagac cgcgcagctc gaacctaccg ctcgaatcgc tcaagccctg
    11521 tctgaacgtg gcagcgttgc cgtcgaccac gtccttgcgc tcaccagtca cctgcgcgcc
    11581 ttcgacggga ttgccggtct gatcgacgac catgccggcg atgctcgcgg tgttctcgag
    11641 aacgagctcg agctcctttg gcgcacccat ggagagatcg atgcgctgcg ccttgctcga
    11701 agcgtcggtg tcgctcgcgc tgatccagat ctcgctgcgc ggcatgccat cgatcacgaa
    11761 cgcaccggtc gcatcgacgt ggctcacgcg tgctccgtct gcccaccaca cgcggaccgc
    11821 ggcgccggga gccggtgtgc gatcgcgatg cacggcacgg cccgccagct gttgatccga
    11881 gacgatgcgg acgacgatcc cgtcgacatc atgatcgacg tggacgacgg cgcttgtcgc
    11941 ggtcgctcga ttcggcgcga tcacgctcac gacgtagctg ccgcgcgcga cacccgcgac
    12001 gtcgaagctg ccatcggcac tgctgcgaac gccatcgcgg cttggatcgg acgtcgatac
    12061 ccagtcgctc gcactctcga gccacacgag tgcgccgtca accggcttct cggcgcgatc
    12121 gatgacgcga ccgcgcgcat gaaacccgcg cgtcagtgcg acggcgactt tgctatgcgc
    12181 cgtcggactg gccgtgacgg cctcgaaggc ggtcgcaaaa ccgggcgcgc tgaccacgag
    12241 cggccagcga cccggaatga tcggcgatag cgtggctgcg ccgtcgcgcg ccgtgttcgc
    12301 cgacgtcacg atcggcgccc gcacctcgac tcttgcctcg gcgatcggtt gtccgcgctc
    12361 gccatcgacg accgtgacgt cgaccgcggc ggcggcacgc agcttcagga ccacgggttc
    12421 ggtct
//
LOCUS       DASUFX010000195         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1003314, whole genome shotgun sequence.
ACCESSION   DASUFX010000195 DASUFX010000000
VERSION     DASUFX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003314"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFV99_07730"
     CDS             <1..907
                     /locus_tag="VFV99_07730"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5059233.1"
                     /translation="ALDNHRRRGQEGVVTELSPGWIRATYYFLLAALAVGIVVSIVVH
                     VPTYSSGYGVVMYPGTPMTAPAPGTVDKIYVEAQTDVKRGMPLVKLKSEKEESDYKQA
                     NTELDAALQSYLFDPADESIRKALVSAQASARRAADAIEQRTVRATVDGTVSDVRVAE
                     GKSVNFGDPILTIVAPGTQPELWALMPGNDRPRFRIGQEMQVELTGFQKKREHAKIFD
                     IALSNTGTTMARNLLGVELADSLKLSPEGGNYVLVKAKLPKNTFKAKGKLYRYYTGMA
                     TKIEVKVEDKRFISTLLPSLEKYVE"
     gene            918..3224
                     /locus_tag="VFV99_07735"
     CDS             918..3224
                     /locus_tag="VFV99_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX5059234.1"
                     /translation="MANNKQIAESYPALRKLGFLRSKRAVPFVQQLEWTDCGAASLCM
                     VMAYHGRETKLAEVREAMGIGRDGVSAKAILDTAEKYGLAGRGIKVDISQIKLLRPAT
                     ILHWEFNHFVVFDRVVKDGVRIVDPATGPRDVPLAQFAKAFTGVAIELTSTPRFTKKK
                     AEKGRLKRYVDELLSEKGLFSRVIIVSLALRLVALALPLITGMIIDQVVMRSDYKLLY
                     VCLASIGAMVVFDLIAEIVRAHLLLHLRIALDTRMTLGFLDHMISLPFSFFQRRSTGD
                     LMMRVDSNGTVREIVTSKSLSAIIDGLFVLLYAIVIFYVSPTLGFITIGMSLLEALVF
                     LCARPTFQRLLAADLDKQAKAHSYMVQMLGGMETLKCAGAERLGLEKWSNLYTDELNV
                     KMRRARAQAYTDGLRGAVAALGPMLILTIGASAVMSGKLSLGEMLAMNSLATSLFGPL
                     SALVASALELQLVKGHMDRIDDVLQSTVEQDRDKVQLPGRLHGHVTVKNLSFKYGEQA
                     PLVVQDVSLDIPPGAAVALVGPSGSGKSTLLNLLSGLYKPTTGDIAYDQRPLHDMDLR
                     GVRQQIGVVPQHPFIFGGTMRENIALGLPGAQLDRISHAAKLACLHDDVAAMPMTYDT
                     VISDGGGSLSGGQRQRVAIARAILRNPTLMLLDEATSALDNQTEAAVIENLERLRCTR
                     ITVAHRLSTIRNADLIIVMDKGRVIEQGTYAQLAARGGLFSQLLAASQSSPNNVEIPY
                     EAAFAGQVPRSSAPRSSMQRGRNSAERA"
     gene            3136..4026
                     /locus_tag="VFV99_07740"
     CDS             3136..4026
                     /locus_tag="VFV99_07740"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5059235.1"
                     /translation="MKPHSLVKCLAALLLVAACSGGETAPNEPDPTEAVHEAAKPEAP
                     KAPEFVGVISSRVSKVIAADFDSKVERLNIRNGQRVKEGEIVAELATEELQAKLAQAK
                     GTFDAAKGQRARAGAAYANAARRARLEQRLIRSGASAPESLNNIRAESSQFGAEGSSA
                     AGEMARAAAQIKELEALLARAKVPAPIDGVVSIVKAREGANLSKAEQIARVFDPNQLV
                     VRFAVPKKHLSLVKPGTAIELVTTEGHRVVPATVRVQDDTADPTIDFTVFEAVIDPTF
                     RTDEIRVGDNGHVRIAGAAK"
     gene            4050..5465
                     /locus_tag="VFV99_07745"
     CDS             4050..5465
                     /locus_tag="VFV99_07745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5059236.1"
                     /translation="MDGQDLETPTWVTSYQGLPRTVNVRRCRLEVVAGVDQGKVLEIA
                     QPSIQIGRVGADLNLTDGKVSGLHCELKLQPEGYRLRDLGSTNGTFVKGVRVVDAFIA
                     PGSTIQIGKSAVTFNPLDDAVAMPLWNEARLHNLIGGSASMRHMFELINRFAQSDATV
                     LIQGETGAGKELVADAIHQCSPRRNEPFIVLDCSAIPEQLFEAQLFGHEVGAFTGAVK
                     STAGVFEMAHGGTLFLDEIGELPLDVQAKLLRAVETRKVRRLGGAKLFASDVRLIAAT
                     NRDLAAEVNRGNFRADLYYRLAVARLAVPPLRERREDLPLLVDHFLRQLSVTSGHPDP
                     RLPDDFMSRANRHTWPGNVRELRNSVERAVLLPNHPNVAFETPPKKEGNGFAHIDIEV
                     PFKVAKQKLVDEFDRRYLEALLEAHDGNISAAARAAGIERMSIYKMIRRLGLDKEEGE
                     TGSRAPTADEDEDTEDTGHSR"
     gene            5489..6190
                     /locus_tag="VFV99_07750"
     CDS             5489..6190
                     /locus_tag="VFV99_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059237.1"
                     /translation="MWRVIVVALLAACSASSSSPRASEPPAPPPRAVVPTGAPAPAAL
                     PFTSTCVAAADPSLADLRQRAGAATKIGFEEALARNHLRVLVLAVHADEIGEGLQEMA
                     AHAPEGTAVKRTLDGAKGMFVAAETTWSGDPGSPAAWEFVQSDRGDMYRLIRKPIAGV
                     TRVLLCGCREHRCGPPGSGCPACGSTAQTMYGPLPAGTHYKGELELAYTANIVSTEYE
                     QTQGCPPPPDCPKPP"
BASE COUNT         1134 a         2088 c         1958 g         1013 t
ORIGIN      
        1 ggcgctcgac aaccatcgcc ggcgcggcca ggaaggtgtt gtcaccgagc tgtcgcctgg
       61 ctggattcgc gcgacctact actttctgct cgcggcgctc gcggttggca tcgtcgtgtc
      121 gatcgtggtc cacgtgccga cgtattcgtc gggttacggc gtcgtcatgt atccgggcac
      181 gccgatgacg gcgcccgcgc cgggcacggt cgacaagatc tacgtcgagg cgcagaccga
      241 cgtgaagcgg ggcatgccgc tcgtgaagct caagtccgag aaagaagaat cggactataa
      301 gcaagcgaac accgagctcg atgccgcgtt gcaatcgtat ctgttcgatc ctgccgacga
      361 atcgatccgc aaagcgctcg tgagcgcgca ggcctccgcg cgccgcgccg ccgacgcgat
      421 cgaacagcgc accgtgcgcg cgacagtcga tggcaccgtc agcgatgtcc gcgtcgcgga
      481 gggcaagtcg gtcaacttcg gtgatccgat cctgacgatc gtcgcaccgg gcacgcagcc
      541 cgagctgtgg gcgctgatgc ccggcaacga tcgtccgcgc tttcgcatcg gtcaggagat
      601 gcaggtcgag ctcacgggct tccagaagaa gcgcgagcac gcgaagatct tcgacatcgc
      661 gctgtcgaac accggcacga cgatggctcg caacctcctc ggtgtcgagc tcgcggactc
      721 gctcaagctc agtcccgaag gcggcaatta cgtgctcgtg aaggcgaagc tgccgaagaa
      781 cacgttcaag gcgaagggca agctgtaccg ctactacacc ggtatggcga cgaagatcga
      841 agtgaaggtc gaagacaagc gcttcatctc gacgctgctg ccatcactcg agaagtacgt
      901 cgagtaaggg gaacgccatg gccaacaaca aacaaatcgc cgaaagctac cccgcgctcc
      961 gcaagctcgg cttcctgcgc agcaagcgcg cggtcccgtt cgtccagcag ctcgagtgga
     1021 ccgactgcgg tgcagcctcg ctctgcatgg tgatggcgta tcacggccgc gagaccaagc
     1081 tcgccgaagt tcgcgaggcg atgggcatcg gtcgcgacgg tgtcagcgcg aaggcgatcc
     1141 tcgacaccgc cgagaagtac ggtctcgcag gtcgcggcat caaggtcgac atctcgcaga
     1201 tcaagctgct caggccggca acgatcctgc actgggagtt caaccacttc gtcgtgttcg
     1261 atcgcgtcgt caaagacggc gtgcgtatcg tcgatccggc gaccggtccg cgcgacgtgc
     1321 cgctcgcgca gttcgcgaag gcattcaccg gcgtcgcgat cgagctgacc tcgacgccgc
     1381 gcttcacgaa gaagaaggcg gagaagggcc ggctcaagcg ttacgtcgac gagctgctgt
     1441 ccgagaaggg cctgttctcg cgcgtgatca tcgtgtcgct cgcgctgcgc ctcgtcgcgc
     1501 tcgcgctgcc gctgatcacc ggaatgatca tcgaccaggt cgtgatgcgc agcgactaca
     1561 agctgctcta tgtctgtctc gcctcgatcg gcgcgatggt cgtgttcgat ctgatcgccg
     1621 agatcgtgcg cgcgcacctg ctgctgcact tgcgcatcgc gctcgacacg cggatgacgc
     1681 tcggcttcct cgatcacatg atctcgctac cgttctcgtt cttccagcgt cgctcgacgg
     1741 gcgatctgat gatgcgcgtc gactcgaacg gcaccgtgcg cgagatcgtc acgagcaaga
     1801 gcctgtccgc gatcatcgac ggcttgttcg tgctgctcta cgcgatcgtc atcttctacg
     1861 tcagcccgac gctcggcttc atcacgatcg gcatgtcgct cctcgaggcg ctcgtgtttt
     1921 tgtgcgcacg gccgacgttc cagcgcctgc tcgccgctga cctcgacaag caagcgaagg
     1981 cgcatagcta catggtccag atgctcggcg gcatggagac gctcaagtgc gccggcgccg
     2041 agcggctcgg cctcgagaag tggagcaacc tctacaccga cgagctcaac gtcaagatgc
     2101 gccgtgcccg cgcgcaggcg tacaccgacg gtctgcgcgg cgcagtcgcc gcactggggc
     2161 ccatgctgat cctcacgatc ggcgcgagcg cggtgatgag cggcaagctc tcgctcggcg
     2221 agatgctcgc gatgaactcg ctggcgacga gcctgttcgg cccgctctcg gcactggtcg
     2281 cgagcgcgct cgagctgcag ctcgtcaaag gccacatgga tcgcatcgac gacgtgcttc
     2341 agagcacggt cgagcaggat cgcgacaagg tccagctgcc gggccgcctg cacggtcacg
     2401 tcacggtgaa gaacctgtcg ttcaagtacg gcgagcaagc gccgctcgtc gtgcaggacg
     2461 tctcgctcga tattccgccg ggcgctgcgg tcgcactcgt cggtccgtcc ggctcgggca
     2521 agtcgacgct gctcaacttg ctctccggtc tctacaagcc gacgaccggt gacatcgcgt
     2581 acgaccagcg cccgcttcac gacatggatc tgcgcggcgt gcgccaacag atcggcgtgg
     2641 tgccgcagca cccgttcatc ttcggcggca cgatgcgcga gaacatcgca ctcggcctac
     2701 ccggcgcgca gctcgatcgc atctcgcatg ccgcgaagct cgcgtgtctg cacgacgacg
     2761 tcgcggcgat gccgatgacc tacgacaccg tgatctccga tggcggtggc tcgctctcgg
     2821 gtggtcagcg ccagcgcgtc gcgatcgccc gcgcgatcct gcgcaacccg acgctaatgc
     2881 tgctcgacga agcgacgagt gcgctcgaca accagaccga agccgccgtc atcgagaacc
     2941 tcgagcgcct gcgctgcacg cgcatcacgg tcgcccaccg tctctctacg atccgcaacg
     3001 ccgacctcat catcgtgatg gacaagggtc gcgtcatcga acaaggcacc tacgcccagc
     3061 tcgccgcgcg cggcggtctg ttctcgcaac tactcgcagc ctcgcagtcc tcccccaaca
     3121 acgtggagat tccctatgaa gccgcattcg ctggtcaagt gcctcgcagc tctgctcctc
     3181 gtagcagcat gcagcggggg cgaaacagcg ccgaacgagc ctgatccgac cgaggccgtt
     3241 cacgaagcgg ccaagccgga agctccaaag gcgcccgagt tcgtcggtgt catcagctcg
     3301 cgtgtctcga aagtgatcgc ggcggacttc gacagcaagg tcgagcggct caacatccgc
     3361 aacggccagc gcgtcaagga aggcgaaatc gtcgccgagc tcgccaccga ggagctgcag
     3421 gccaagctcg cgcaggccaa gggcacgttc gacgccgcga agggccagcg tgctcgcgcc
     3481 ggtgcggcct acgcgaacgc cgcgcgccgc gctcgcctcg agcagcggct catccgcagc
     3541 ggagcctccg cgcccgaatc gctcaacaac atccgcgccg aatcgagtca gttcggtgcc
     3601 gagggctcga gcgccgcggg cgagatggcg cgcgccgccg cgcagatcaa agagctcgag
     3661 gccctactcg cccgcgccaa ggtccccgcg ccgatcgatg gcgtcgtctc gatcgtgaaa
     3721 gcgcgcgaag gcgccaacct gtccaaggca gagcaaatcg cacgcgtgtt cgatccgaac
     3781 cagctggtcg tgcggttcgc agtgcccaag aaacatctct ccctcgtcaa gcccggtacc
     3841 gcgatcgagc tggtcaccac cgaaggacac cgtgtggtgc ccgcaacggt gcgcgtccag
     3901 gacgacaccg ccgatccgac gattgacttc accgtgttcg aagccgttat cgacccaact
     3961 ttccgcaccg acgagatccg cgttggtgac aacggacatg tccggatcgc aggagccgca
     4021 aaatgagcgt ggtacgatca caagaaatcg tggatggcca ggatctcgag accccgacct
     4081 gggtgacttc gtatcagggt ctgccgcgaa cggtgaacgt tcgacgttgc aggctcgaag
     4141 tcgtcgccgg tgtcgatcaa ggcaaggtcc tcgagatcgc gcagccatcg atccagatcg
     4201 gtcgcgtcgg cgccgatctc aatctgaccg acggcaaggt ctcgggcctg cactgcgagc
     4261 tcaagctcca gcccgaaggc tatcgcctgc gcgacctcgg ttcgacgaac ggcacattcg
     4321 tcaaaggtgt gcgcgtggtc gacgcgttca tcgcgccggg ctccacgatc cagatcggca
     4381 agagcgcggt cacgttcaac ccgctcgacg acgcggtggc gatgccactg tggaacgaag
     4441 cgcggctcca caacctgatc ggtggcagcg cgtcgatgcg gcacatgttc gagctgatca
     4501 atcgcttcgc gcagtccgac gcgactgtcc tcatccaagg tgagaccggt gccggcaagg
     4561 agctcgtcgc cgacgcgatc catcagtgct cgccacgccg caacgagccg ttcatcgtgc
     4621 tcgactgctc cgcgatcccc gagcaactgt tcgaagcgca actcttcggt cacgaggtcg
     4681 gcgcgttcac cggcgcggtc aagtcgacgg caggcgtctt cgagatggcg cacggcggca
     4741 cgctcttcct cgacgagatc ggcgagctgc cgctcgacgt ccaggccaag ctcctgcgcg
     4801 cggtcgagac tcgcaaggtc cgtcgcctcg gtggtgcgaa actcttcgca tccgacgtcc
     4861 gcctcatcgc ggcgacgaac cgcgatctcg cggccgaggt gaaccgcggc aacttccgcg
     4921 cggatctcta ctaccggctc gcggtcgccc gtctcgccgt gccgccgctg cgcgagcgtc
     4981 gcgaagacct gccgctgctc gtcgatcact tcctgcgcca gctcagcgtc acctcgggtc
     5041 atcccgatcc gcgcctgccc gatgacttca tgtcgcgcgc aaatcgccac acgtggccgg
     5101 gcaacgtgcg cgagctgcgt aactcggtcg agcgcgccgt gctgctgccc aaccatccga
     5161 acgtcgcgtt cgagacgccg ccgaagaagg aaggcaacgg gttcgcgcac atcgatatcg
     5221 aggtgccgtt caaggtcgcg aagcagaagc tcgtcgacga gttcgatcgc cgctacctcg
     5281 aagcgctcct cgaggcacac gacggcaata tctcagccgc cgcgcgtgcc gccggtatcg
     5341 agcgcatgtc gatctacaag atgatccgcc gcctcggcct cgataaggag gaaggtgaaa
     5401 cgggcagccg tgccccgacc gccgacgagg acgaggacac cgaagacacc ggccacagcc
     5461 gctagcaaag ccgtgccatc atcgacgcgt gtggcgcgtc atcgtggtgg cactgctcgc
     5521 agcatgctcg gcatcctcgt catcaccacg cgcgagcgaa ccgcccgcgc cgccgccacg
     5581 tgcggtcgtg ccgacgggtg ctccagcccc tgccgcgctg ccattcacgt ccacgtgcgt
     5641 cgccgcggcc gatccgtcgc ttgcggatct gcggcagcgc gctggtgcag cgaccaagat
     5701 cggcttcgag gaggcgctcg cgcgcaatca cctgcgtgtg ctcgtgcttg ccgtgcatgc
     5761 agacgagatc ggcgaaggct tgcaggagat ggcagcgcac gcgcccgagg gcactgctgt
     5821 caaacgaacg ctcgacggcg cgaagggcat gttcgtagcc gcagagacaa catggagcgg
     5881 agatcccggg agccccgcgg cgtgggagtt cgtgcagagc gatcgcggcg acatgtatcg
     5941 gctgatccgc aagccgattg ctggcgtcac gcgcgtacta ctgtgtggtt gtcgcgagca
     6001 ccgatgtgga ccgccgggat cgggatgtcc ggcttgtgga tcgacggcgc agacaatgta
     6061 cgggccgctg ccggccggta cgcattacaa gggcgagctc gagctggcgt atacggcgaa
     6121 tatcgtgtcc acggagtacg agcaaacgca aggatgcccg ccgccaccag actgtcctaa
     6181 gccgccgtaa tgg
//
LOCUS       DASUFX010000196         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1141315, whole genome shotgun sequence.
ACCESSION   DASUFX010000196 DASUFX010000000
VERSION     DASUFX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141315"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFV99_07755"
     CDS             <1..604
                     /locus_tag="VFV99_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX5059238.1"
                     /translation="DLVDTPHGRALILELDDAAAEPLVGAEVAIAAALGPVRKREFVA
                     GRTALRSLLGEPVAIVPDERGAPMLPAGWVGSISHKHDRAAALVAPAGDGFVGVDIEI
                     AGPPRMPIEKRILTAREQHRIRDGRDVTLHFAIKEAIYKAIDPMVRRYVGFTEVELEI
                     AADGSCSVHAVDAARLPVTIEAWWREHDGHWLATARGRKR"
     gene            932..1270
                     /locus_tag="VFV99_07760"
     CDS             932..1270
                     /locus_tag="VFV99_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059239.1"
                     /translation="MGRPPVAGRDLGFRGQQFPFFFRCRDVDVLPLFDEDLDDLAVRK
                     RNVADHDLALLDGRCKDHGHTSSVAQTRGGPQQRLRQRNVAISRDAASGSSVTRTRFV
                     RCSSNTGQWR"
     gene            complement(1267..1818)
                     /locus_tag="VFV99_07765"
     CDS             complement(1267..1818)
                     /locus_tag="VFV99_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059240.1"
                     /translation="MAETWRKCSACKNPISFNAKYWACSVSTCNRPRTGYVFCTVSCW
                     DSHIAEARHRDAWADERRAPTPEAWAKEMAANPEPAPVAPKPVAPQGSAPVRRVVGDA
                     SPATAAAVEGNVQLADAEREVLIVVSKMKKYIKDRSGFNTSDAVAEMLSDHVRAICDD
                     AIRAAARDERKTVLDRDVPKPRR"
     gene            complement(1830..3047)
                     /locus_tag="VFV99_07770"
     CDS             complement(1830..3047)
                     /locus_tag="VFV99_07770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5059241.1"
                     /translation="MRFPVALALFSLPAVACTIDDIDVSVTDQYVIGGAAAPAGKWPD
                     TVAVMWGTNPTQDQGCTGTLVAPNVVITAGHCVIGGAPTHVLVGATKLSAPQEGQLIP
                     IMKAIEYPNSQGSVDAGVLVLQTPVTGIEPRPIASGWAKFDIQNNAMVQLVGFGTTDK
                     NGSVSTDALMEATTIITDYNCTKEGVGCNTAARPDGEFGAGGSGIDTCPGDSGGPVYL
                     TTSYGTYVMGITSRGYETNNYACSEGGIYERPDKIVDWLDMMSGAKVARGPSPVADPP
                     TIMTIRGEGAETKIAHNDPKMGVTHKFEITKPPGFGKAAVRDDGTLRVCPNKDVAGDD
                     IVEVTVSDTADTTRAITLKVPIVIADGSPPDSCDVNDFGGDGGGCCDTRRSAGGSIPL
                     GLVVLLALRRRRK"
     gene            3157..>3873
                     /locus_tag="VFV99_07775"
     CDS             3157..>3873
                     /locus_tag="VFV99_07775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5059242.1"
                     /translation="MRWVSFVVLVACGGTTKSVAPVKPAGDTDPDGPNRARIAAQVQP
                     LIDAELATGVVVGVIDGNKKEIYGFGKGPGGKPPTGATLFELGTVTRAYTGLLLADAV
                     QRREVELDTPVAELLPTGVTAPTKDKLVITLRHLALHSSGLPPIPPSLPAESDNPFGS
                     YRENQLYQDLIGTRLIGAPGERIVYSDYGVGLLANALGHKIGGGYVKALDERVLAPLG
                     LRDTYFAVPTPAQPRLASGTN"
BASE COUNT          604 a         1341 c         1263 g          665 t
ORIGIN      
        1 tgatctcgtc gacactccgc acggccgcgc gctgatcctc gagctcgacg acgcggcggc
       61 cgaaccgctc gtcggcgccg aggttgcgat cgccgctgcg ctcggcccgg tccgcaagcg
      121 cgagttcgtc gccgggcgca ccgcgctgcg cagcctgctc ggcgagccgg tcgcgatcgt
      181 acccgacgaa cgcggcgcgc cgatgctgcc cgccggctgg gtcggctcga tcagccacaa
      241 gcacgaccgc gccgcagcgt tggtcgcacc cgccggcgac ggcttcgtcg gtgtcgatat
      301 cgagatcgca gggccgccgc gcatgccgat cgagaagcgc atcctgaccg cgcgtgagca
      361 acaccgcatt cgcgatggcc gcgacgtcac gctgcacttc gcgatcaagg aagcgatcta
      421 caaagcgatc gatccgatgg tgcgccggta cgtcggcttc accgaggtcg agctcgagat
      481 cgcggccgac ggcagctgca gcgtgcacgc cgtcgacgcg gcgcgcttgc cggtcacgat
      541 cgaggcctgg tggcgcgagc acgacggcca ctggctcgcc acggcgcgcg gccggaagcg
      601 ctagcgtcag cgagcacggc gatgatgccc gtgcattagc cgcgtgattc tcacgatgcg
      661 ctcgtcgtcg tcgacaacgt agaacagcac gaacggcgtg cggtcgagcg gcaaccgaaa
      721 gtgtctgagg cgaccggcag ctcgaacgaa tgttccagcc aggggatagc tggcgagtag
      781 ctcaaccgcg gcttctacct cgtccggaag ctgacttggg ccttggccgc gcagctccca
      841 tctttgcttt tcgcgctcca cggcgcgctc cacgcgcagc aacagctcta ccgcgtgcgg
      901 cgtcccgggc tcggagctcc gcgaagaatt catgggcagg ccgcccgtcg ccggccgcga
      961 tctcggcttc cgcggccagc agttcccgtt cttcttccgg tgtcgggatg tagacgttct
     1021 tcctctgttc gatgaagacc tcgacgacct cgccgtccgc aagcggaacg tcgcggatca
     1081 cgacctcgcc cttcttgacg gtcgctgtaa agaccatggc catacctcca gcgtagcgca
     1141 gacacgcggc ggaccacagc aacgcctgcg ccagcgcaac gtcgcgatct cgcgagatgc
     1201 tgcgtccggt tcgtccgtca cccggacgcg attcgtccgc tgctcgtcga acaccggaca
     1261 gtggcgctag cggcgtggct tcggtacgtc gcgatcgagc acggtcttgc gctcgtcgcg
     1321 ggcggcagca cgaatcgcgt cgtcgcagat cgcgcgcacg tgatcggaca gcatctcggc
     1381 caccgcatcc gaggtgttaa agcccgagcg gtccttgatg tacttcttca tcttcgacac
     1441 gacgatcagg acttcgcgct cggcatcggc gagctgcacg ttgccctcga ccgctgccgc
     1501 ggtcgccggc gacgcatcac cgaccacgcg gcgcaccggc gccgagccct gcggagcaac
     1561 tggcttcgga gcgacgggcg cgggctcggg attcgcggcc atctctttcg cccatgcttc
     1621 gggcgtcggc gcgcgcctct cgtcggccca cgcatcgcga tgccgcgctt ccgcgatgtg
     1681 actgtcccag cagctcaccg tacagaacac gtagcccgta cgcgggcggt tgcacgtcga
     1741 tacgctgcac gcccagtact tcgcgttgaa cgagatcggg ttcttacaag cgctgcactt
     1801 acgccacgtc tcggccatcg agcgcgagac tacttccgac ggcggcgcag tgctagcagg
     1861 acgaccaatc cgagcgggat cgatccgccg gcgctccggc gcgtatcgca gcagccaccg
     1921 ccgtcgccgc cgaagtcgtt cacgtcgcag ctatccggcg gcgagccatc ggcaatcacg
     1981 atcggcacct tgagcgtgat ggcgcgcgtc gtatcggccg tgtcgctgac tgtcacttcg
     2041 acgatgtcgt cgccggccac atccttgttc gggcagacgc gcagcgtacc gtcgtcgcgc
     2101 acggcggcct tgccgaagcc cggcggcttc gtgatctcga acttgtgggt gacgcccatc
     2161 ttcggatcgt tgtgcgcgat cttcgtctcg gcgccttcgc cacggatcgt catgatcgtc
     2221 ggcggatcgg cgaccggcga cgggccgcgc gcgaccttcg cgcccgacat catatcgagc
     2281 cagtcgacga tcttgtccgg gcgctcgtag atgccgccct cggagcaggc gtagttgttc
     2341 gtctcgtagc cgcgcgacgt gatgcccatg acgtacgtgc cgtagctcgt cgtcaggtag
     2401 accgggccgc cggaatcgcc ggggcaggtg tcgatgccac tgccgccggc gccgaactcc
     2461 ccgtcggggc gtgcggccgt gttgcagcca acgccttcct tcgtgcagtt gtagtcggtg
     2521 atgatcgtcg tcgcttccat cagcgcgtcg gtcgatacac tgccgttctt gtcggtcgtg
     2581 ccgaagccga cgagctggac catcgcgttg ttctggatat cgaacttcgc ccagccgctc
     2641 gcgatcgggc gcggctcgat tccggtgact ggcgtctgga gcacgagcac gccagcgtcg
     2701 accgagccct gcgagttcgg atactcgatc gccttcatga tcgggatcag ctgaccctct
     2761 tgtggcgccg acagcttcgt cgcgcccacg agcacgtggg tcggcgcgcc gccgatcacg
     2821 cagtggcctg cggtgatcac gacgttcggc gcgacgagcg taccggtgca gccctgatcc
     2881 tgcgtcgggt tcgtgcccca catcaccgcg acggtgtcgg gccacttgcc cgccggtgcg
     2941 gcagctccgc cgatgacgta ctgatcggtc accgagacat cgatgtcgtc gatggtgcag
     3001 gcgacggcgg gcagtgagaa cagcgcgagc gcgaccggaa accgcatggg ccgactgtta
     3061 cacgatccgt aaccctcgca ctatgaggcg tttcacatgc gctagcgcgg tgttgcgcag
     3121 gatgcgcaag cccgtcaatc taccgtctcc atgtccatgc ggtgggtctc gttcgttgtt
     3181 ctagttgcgt gcggcggcac gacaaaatcg gtcgcgccgg tgaagcctgc gggtgacacc
     3241 gatcctgacg gaccgaatcg tgcgcgcatc gccgcccagg tccagccgct gatcgatgcc
     3301 gagctcgcga ccggtgtcgt cgtcggcgtg atcgacggta acaagaagga gatctacggg
     3361 ttcggcaaag ggccgggcgg caagccaccg acgggcgcga cgctgttcga gctcggcacg
     3421 gtgacgcggg cgtataccgg gcttctgctt gccgacgcgg tgcaacgccg cgaggtcgag
     3481 ctcgacacgc cggtggccga gctgttgccg accggcgtca ccgcgccgac caaggacaag
     3541 ctcgtcatca cgctgcgtca cctcgcactg cacagctcgg gcttgccacc gattccgccg
     3601 tcgctgccag ccgagagcga caatccattc ggcagctatc gcgagaacca gctctatcag
     3661 gatctgatcg gtacccgact catcggcgcg cccggcgagc gcatcgtgta ctccgactat
     3721 ggcgtcggcc tgctcgcaaa tgcactcggc cacaagattg gcggcggcta cgtcaaggcg
     3781 ctcgacgagc gcgtactcgc gccgctcggc ctgcgcgaca cgtactttgc ggtgccgacg
     3841 ccggcgcagc cgcgcctcgc gagcggcacg aac
//
LOCUS       DASUFX010000197         9262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1003812, whole genome shotgun sequence.
ACCESSION   DASUFX010000197 DASUFX010000000
VERSION     DASUFX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003812"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFV99_07780"
     CDS             complement(<1..741)
                     /locus_tag="VFV99_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5059243.1"
                     /translation="MKKLLLVALFACSSHKSDPAPVKAAAPVASPAPVAAAAQPEAKP
                     EAKPAAPIAKGRVETKRFKSAALGVEKKYLVYLPAGYDSATTRYPVFYYLHGLGGNET
                     NWVEGGKLDEAADGLGLQAIVVMPDGDDSFYVDGLAKVDYDACMKDGTGLFIPQKQNK
                     RDTCVRARKYETYITKDLIGHIDATYRTIAKREARAIAGLSMGGFGALMLSMRHQDLF
                     AAAASHSGVDSLIYMGPFPYVAGKIEQMT"
     gene            complement(750..2690)
                     /gene="sppA"
                     /locus_tag="VFV99_07785"
     CDS             complement(750..2690)
                     /gene="sppA"
                     /locus_tag="VFV99_07785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX5059244.1"
                     /translation="MGVRLASVVVVSVALSLFGCNEKPSKLDGEFAKRASGDPWAATF
                     TKKDDGSGDGGGFGGFDLQSMLERIKESIETPGPYESPKKSKDFDEEKPHWGVMPVSG
                     DIVEREAFSLTGGGRGTELRNLAMRLRTLAKDDKLQGLILRVDDLDISMPDLVELRDA
                     MQDFRNAKKQLRCHAESASNATYLVLAACDKIGLAPLGDIAITGPAAMPIHVKPLLDK
                     LAITADFIHVGAYKGAAEPLTRDAPSKEMEETLGAILDRRYQSMVDDIAASRHLDPAT
                     VKTLIDTALFPAPEALFAKLVDDVSAWETFRDEVKAPWTKLEIEPDDKDQLQSMVKVA
                     RFLGAMPPDRPLGDHVAIVYALGDIKDGDGDGVLGARQEIASHTLVSALRALAADDAV
                     KAVVLRIDSGGGSAQASELIWSSIEQLKAKKPVIVSMSDVAASGGYYIASGATKIFAQ
                     ANTLTGSIGVVGGHIAPGAALAKLGVNTFPMGRGKHATMMASFKPWTDDERALMQHHM
                     EAVYNTFVARVAAGRKKTVEQILPIAQGRVWTGTKALELGLVDEIGGLDAAVAEARKL
                     AKVDAEIALEVYPPSPTLRDLLAGWGQVHAPLGVSAEASAIEALRALDPHVALAAERL
                     LALVMSFRTTTIQTVAILPEVH"
     gene            complement(2715..5081)
                     /locus_tag="VFV99_07790"
     CDS             complement(2715..5081)
                     /locus_tag="VFV99_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX5059245.1"
                     /translation="MDVRCEKCQTEYELDESRLKPGGVTVKCTNCGHMFKIRKRANTN
                     VGLAGSSAQANPVDNRRSPSGKQPTVRADSVAEAPVKIADLPSGPTSAGERQWQVRLE
                     NGETKTCRELVALQQWIVAGIATRESLISRTGKTWKRLGDIDELAQYFVIADEARSQR
                     SVKPTPKPPAPAATMLGVGRPGGVDEEEGRATGNYRARPPTPPPPAAPKPSTAPSAIA
                     QTELAPTSQPSMAQKRPPTNPPPAPRVKPPSDAGRQTAMWANNEVKASDSMAAMPQGP
                     RGGKLSAAPEEQAFAGRVRMEVNSSPFVAGGGVRVDDDDDDVYPAAKGSKVGMWIALI
                     SLLVIGAAAGAVYMLVFRNNKTQVAQPTVKDAAVAVATPDATSVVVTPIDAQEVAVSP
                     LDTARAELVAGVWVRMQKAFDDLNGKDDPPTLALRARLMLALAQGKQDRAGYVEKAES
                     DKLRKEAKQLVVDNAPMAQRAQGAAADNASANLAMADVMRLQGKPAPSVKRYIETARS
                     KAAGDKELMASVALADAQLALRDGKLPDAEKALAGIEAPDDMRIKLARAQIWYAQNKK
                     AEAKGQVDQVLATQPDNDVALAMYKKLETSVANTDPMPGEDGSSATKPPTNNGNGATK
                     PPNGGGGGTSGGGGGNDYDSVLAKANKQAETNCTKAMDLYGKALEQRPNGVEALTGMG
                     YCHLDAKQFSSAFSKFRAALAVSSKYEPALGGVAETYQRQGNKEAAVEAWRKYLDAYP
                     NSAKAKKQLEILGAGESPEQPKEQPKEQPQPKDQPAPPPPPPAPGSAG"
     gene            complement(5177..5803)
                     /locus_tag="VFV99_07795"
     CDS             complement(5177..5803)
                     /locus_tag="VFV99_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059246.1"
                     /translation="MLSAPPRNPIPKLADLPLVIETPRVKLRPQVESDADRFFPYVSD
                     PALSQYLTWSAHSSIDETREWLKKVTEMVAAGTDVVWTIEHEGAPVGCIGLHGIQWVM
                     RAVRCDHAEMGYWIAKPFWGKGLMTEAATAATRWAFETLGLHKVNIRCFETNVGSQRV
                     MEKIGFRFLCRAEEDVWRDGRWYASLRYELTASEWADSTRTLRFTRPR"
     gene            complement(5843..6202)
                     /locus_tag="VFV99_07800"
     CDS             complement(5843..6202)
                     /locus_tag="VFV99_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEX5059247.1"
                     /translation="MTSENESRPPKTHRLILPLGMRVLVQLIRDEDRTDAGLYLPQGA
                     KEAQDDAHYGKVIEVARDRPTTDEATENVSGVPLGAHVLFRKDAGVRVPWDERLRIID
                     VKDILATVEEVHPDDAH"
     gene            complement(6192..6749)
                     /locus_tag="VFV99_07805"
     CDS             complement(6192..6749)
                     /locus_tag="VFV99_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059248.1"
                     /translation="MSGAVCPSCGVAVVPGYVRCPKCHKALPRARGSHLEGGTAVASA
                     TGKGPLITAIAIGVVAIGAIAYLGLRKNEKPAAQPAATVETPAVPAQAEVETPAQPTE
                     QPAQPTGPTALDVAAGLERNLKKQRLWSTVSIIGDHVDVRSGSCEDPGMKPALDEVAP
                     SFKAAGLTSLRCLEQSGRVVTARDL"
     gene            complement(6768..7916)
                     /locus_tag="VFV99_07810"
     CDS             complement(6768..7916)
                     /locus_tag="VFV99_07810"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEX5059249.1"
                     /translation="MRSFVLVVALTGSATAAPMTVSGDVSDVRSHWTADGAQIVTEAT
                     VHTASGDVVVNQLGGTVDGLTMRQFPGDEPLAVGMRVTVAVRPSMDLSQRMHNAVEGV
                     KVDAYPSNYVRTGPTKAGHYLFWESGCVFVTLDSAGTKEIAGDGEFPVIDQAIATWNN
                     GVASCSYMQIINAGRKAAEVDGRDRVNLIKFRDTSWCRPATQDDPARCHPDSAAGITT
                     ATYVDDAGSSRDGAIVDADIELNGKNFAISVNGVTLGTASCLSELQNTLTHELGHLLG
                     LEHTCLATGDPPRVDDKGNAVPSCGNATKTQQDATMFNFQDCGENKKETLEPDDIAGI
                     CGIYPTAQDPGTCEPVDDNTGCCSTGSGAAGPLALTGLLMLGFWRRRR"
     gene            7956..>9262
                     /gene="gltX"
                     /locus_tag="VFV99_07815"
     CDS             7956..>9262
                     /gene="gltX"
                     /locus_tag="VFV99_07815"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5059250.1"
                     /translation="MRPVRVRIAPSPTGDPHVGTAYIALFNYVFAKQQGGKFVLRIED
                     TDQTRARADSEQMIFDALRWVGLSWDEGPDVGGPYGPYRQSERSELYLTHVGMLLESG
                     AAYRCFCTAERLEKVRVQQQAEKATLGYDRHCRNLDPDEARNRAAQGEAHVVRLKVPL
                     SGPIEFTDKLRGKVTRDAKEIDDQVLLKSDGLPTYHLANVVDDHLMEITHVIRAEEWI
                     SSTQKHVLLYRAFGWDEPEFIHMPLLRNKDKSKISKRKNPVSINYYRDIGILPHALLN
                     FLGMLGWSFGGDREKFTLQEMIDVFSWDRISLGGPVFDLDKLTWLNEKYIHDLNYEQF
                     ADAVIDWRLNKDFLVKVLPLVRERVKKLADIVPATEYFFSGDLDYTAVLPELTIPGVE
                     AKVVSKGVLDFVERFEAKDGFAKEMLEEVARAWAEAQGWKSGQA"
BASE COUNT         1416 a         3135 c         2994 g         1717 t
ORIGIN      
        1 ggtcatctgc tcgattttgc cggccacgta cgggaacggt cccatgtaga tcagcgagtc
       61 gacgcccgag tgactcgccg ccgcggcgaa caagtcttgg tgacgcatcg acagcatcag
      121 cgcaccgaat ccacccatcg acagcccggc gatcgcgcgc gcctcgcgct ttgcgatcgt
      181 gcggtacgtc gcgtcgatgt gaccgatcag gtccttcgtg atgtacgtct cgtacttgcg
      241 agcgcgcacg caggtgtcgc gcttgttctg cttctgcggg atgaagaggc cggtgccgtc
      301 cttcatgcac gcgtcgtagt cgaccttcgc gagtccatcg acgtagaagc tatcgtcgcc
      361 gtcgggcatc accacgatgg cctgcaggcc gaggccatct gcggcttcgt cgagcttgcc
      421 tccctcgacc cagttcgtct cgtttccacc gagtccgtgc aggtaataaa agaccgggta
      481 gcgcgtcgtc gcgctgtcgt agccggccgg caggtacacg aggtacttct tctcgacgcc
      541 gagcgccgcg ctcttgaagc gcttcgtctc gacccgcccc ttcgcgatcg gagcggccgg
      601 cttcgcttcg ggcttggctt ccggttgcgc agctgccgct actggtgccg gcgacgcaac
      661 cggagccgcg gccttcaccg gggccggatc gctcttgtgc gacgaacacg cgaacaacgc
      721 tacgagtagg agcttcttca acttcatcat cagtgcacct ccggcaggat cgccacggtc
      781 tggatcgtcg tcgtgcggaa tgacatcacg agcgcgagca agcgctccgc cgcaagtgcg
      841 acgtgtggat cgagcgcacg cagcgcttcg atcgcggacg cctcggcgct cacacccaat
      901 ggcgcgtgga cttgtcccca accggcgagc aaatcgcgca gcgtcggcga cggcggatag
      961 acttcgagcg cgatctcggc atcgaccttc gcgagcttgc gcgcttcggc aaccgctgca
     1021 tcgagcccgc cgatctcgtc gacgagaccg agctcgagcg ccttggtgcc ggtccacacg
     1081 cgaccctgcg cgatcggcag gatctgctcg acggtcttct tgcgccctgc cgcgacgcgc
     1141 gcgacgaacg tgttgtagac cgcctccatg tgatgctgca tgagcgcacg ctcgtcgtcg
     1201 gtccacggct tgaagctcgc catcatggtc gcgtgcttgc cacggcccat cgggaacgtg
     1261 ttgacgccga gcttcgcgag cgcggcaccg ggcgcgatgt ggccgccgac gacaccgatc
     1321 gagccggtga gcgtgttcgc ctgcgcgaag atcttcgtcg cgccgctcgc gatgtagtag
     1381 ccgccgctgg cggcgacgtc gctcatcgac acgatcaccg gcttctttgc cttgagctgc
     1441 tcgatcgacg accagatcag ctccgacgcc tgcgcactgc caccgcccga gtcgatacgc
     1501 agcacgactg ccttcaccgc gtcgtcggcg gcgagcgcgc ggagcgccga gaccagcgtg
     1561 tgcgaagcga tttcctggcg cgcaccgagc acaccatcgc cgtcgccgtc cttgatgtcg
     1621 ccgagcgcgt acacgatcgc gacgtgatcg ccgagcgggc ggtccggcgg catcgcgccg
     1681 aggaagcgcg cgaccttcac catcgactgc agctggtcct tgtcgtcggg ctcgatctcg
     1741 agcttcgtcc acggtgcctt cacctcgtcg cggaacgttt cccacgccga gacgtcgtcg
     1801 acgagcttcg cgaagagtgc ctcgggggcg ggaaacagcg cggtgtcgat cagcgtcttg
     1861 accgtcgccg gatcgaggtg ccgcgacgcc gcgatgtcgt cgaccatcga ctgataacgg
     1921 cgatcgagga tcgcaccgag cgtttcctcc atctccttcg acggcgcgtc gcgcgtcagc
     1981 ggctcggcgg cgcccttgta cgcgccgacg tggatgaagt ccgcggtgat cgcgagcttg
     2041 tcgaggagcg gcttcacgtg gatcggcatt gcggctgggc ccgtgatcgc gatgtcgccg
     2101 agcggcgcga gaccgatctt gtcgcacgcg gcgagcacga gatacgtcgc gttgctcgcg
     2161 ctctcggcgt ggcagcgcag ctgcttcttc gcgttgcgga agtcctgcat cgcgtcgcgc
     2221 aactcgacga ggtcgggcat gctgatgtcg agatcgtcga cgcgcaggat caggccctgc
     2281 agcttgtcgt ccttcgcgag cgtgcgcaga cgcatcgcca ggttgcgcag ctcggtgcca
     2341 cgcccgccgc cggtgagcga gaacgcttcg cgctccacga tgtcgccgga gaccggcatc
     2401 acgccccagt gcggcttctc ttcgtcgaag tccttcgact tcttcggcga ctcgtacggg
     2461 ccgggcgtct cgatgctctc cttgatccgc tcgagcatgc tctgcagatc gaagccaccg
     2521 aagccaccgc cgtcgccact accgtcgtcc ttcttcgtga acgtcgcggc ccacggatcg
     2581 ccggacgcgc gctttgcgaa ttcaccgtcg agctttgacg gcttttcgtt gcagccgaag
     2641 agcgagagag cgaccgaaac aacaacaacg ctcgcgaggc ggacacccat gcccgcttca
     2701 tatcattaag ccgtctaacc ggcggagccg ggagcagggg gcggcggcgg tggggccggc
     2761 tggtctttgg gctgcggctg ctctttcggt tgttcttttg gctgctcggg cgactcgccg
     2821 gcgccgagga tctcgagctg cttcttagcc ttagctgaat tcgggtacgc gtcgaggtac
     2881 ttgcgccacg cctcgaccgc cgcttccttg ttgccctgac gctgatacgt ctcggcgacg
     2941 ccgccgagcg ccggctcgta cttcgacgac accgcgagcg ccgcgcggaa cttcgagaac
     3001 gcgctcgaga actgcttcgc atcgaggtgg cagtagccca tgccggtcaa cgcctcgacg
     3061 ccgttcgggc gctgctcgag cgccttgccg tacagatcca tcgccttcgt gcagtttgtc
     3121 tcggcctgct tgttcgcctt cgcgagcacc gagtcgtagt cgttgccgcc gccgccaccc
     3181 gacgtgccac caccaccacc gttgggcggt ttcgtcgcgc cgttgccgtt gttcgttggc
     3241 ggcttcgtcg cactggagcc gtcctcgccg ggcatcggat cggtattcgc gaccgacgtc
     3301 tcgagcttct tgtacatcgc gagcgcgaca tcgttgtcgg gttgcgtcgc gagcacctga
     3361 tcgacctggc ccttcgcctc ggccttcttg ttctgcgcgt accagatctg cgcgcgcgcg
     3421 agcttgatgc gcatgtcgtc cggcgcttcg atgccggcga gcgccttctc ggcgtcgggc
     3481 agcttgccat cgcgcaatgc gagctgcgcg tcggcgagtg cgacggacgc catcagttcc
     3541 ttgtcacccg ccgccttgct gcgcgcggtc tcgatgtagc gcttcaccga cggtgccggc
     3601 ttgccctgca ggcgcatcac gtcggccatc gcgaggttcg cgctcgcgtt gtcggcggcc
     3661 gcgccttgcg cacgctgcgc catcggcgcg ttgtcgacga cgagctgctt cgcttctttg
     3721 cgcagcttgt cggattccgc tttctcgacg tagccggcgc gatcctgctt gccctgcgcg
     3781 agcgcgagca tcagtcgtgc acgcagcgcg agcgtcggcg gatcatcttt gccgttcaga
     3841 tcgtcgaacg ccttctgcat ccgcacccac acgccggcga ccagctcggc gcgcgcggtg
     3901 tcgagcggtg acaccgcgac ttcttgcgca tcgatcggcg tcaccaccac gctcgtcgcg
     3961 tcaggtgttg cgaccgcgac ggcggcatcc ttcacagtcg gctgcgcgac ctgggtcttg
     4021 ttgttgcgga acacgagcat gtagaccgcg cccgccgccg caccgatcac gagcagcgag
     4081 atcagcgcga tccacatccc gaccttcgag cccttcgcgg cgggatagac atcgtcgtcg
     4141 tcgtcgtcga cacgcacgcc accaccggcg acgaacggcg agctgttaac ttccatgcgc
     4201 acgcgcccgg cgaacgcttg ctcttcggga gccgctgaga gcttgccgcc gcgcggaccc
     4261 tgcggcatcg ccgccatcga gtcgctcgcc ttgacttcgt tgttcgccca catcgcggtc
     4321 tggcgacccg catccgacgg cggcttcacg cgcggcgctg gtggtgggtt ggttggcggc
     4381 cgcttctgcg ccatcgacgg ctgcgaggtc ggcgcgagct cggtctgtgc gatcgccgac
     4441 ggcgcagtcg atggcttcgg tgctgcgggc ggcggaggtg ttggtggccg cgcccggtag
     4501 ttgccggtcg cgcgaccctc ctcctcgtcg acgccaccgg gacgaccgac gccgagcatc
     4561 gttgccgcgg gagcgggcgg tttgggcgtc ggcttcaccg agcgttgcga gcgcgcctcg
     4621 tcggcgatca cgaagtattg cgcgagctcg tcgatgtcgc cgagccgctt ccacgtcttg
     4681 ccggtgcgcg aaatcagcga ctcgcgcgtc gcgatgccgg cgacgatcca ctgctgcaac
     4741 gcgaccagct cgcgacacgt cttcgtctcg ccattctcga gccgcacctg ccactgccgt
     4801 tcgccggcgg aggttggccc cgatgggaga tctgcgatct tcaccggcgc ttcggcgacc
     4861 gagtcagcgc gcaccgtcgg ctgtttgccc gaaggcgacc tccggttgtc gaccggattc
     4921 gcctgcgcgc tcgatcctgc aagccccacg ttcgtgttcg cgcgcttgcg gatcttgaac
     4981 atgtgcccgc agttggtgca cttgaccgtg acaccaccgg gcttgagtcg actctcgtcg
     5041 agctcgtact ccgtctggca cttctcgcag cgaacgtcca tggactattc ggcggttacg
     5101 gtaccacgac gcacctcgcc gggccacgga gcgcccccgc tggtgcgcag ccgatttatt
     5161 ccaccgacaa agggtcttac cgcggtcggg tgaagcgcaa agtgcgcgtc gagtcggccc
     5221 attccgacgc ggtcagctcg tagcggaggc tggcgtacca gcggccatcg cgccagacat
     5281 cctcctcggc gcggcagaga aacctaaacc cgatcttctc catcacgcgc tgcgagccga
     5341 cgttggtctc gaagcaccgg atgttcacct tgtggaggcc gagcgtctcg aacgcccacc
     5401 gcgtcgccgc cgtcgccgcc tcggtcatca ggccttttcc ccagaatggc ttcgcgatcc
     5461 aatagcccat ttctgcgtga tcgcaacgca ccgcgcgcat gacccactgg atgccgtgca
     5521 ggccgatgca gccgaccggc gcgccctcgt gctcgatcgt ccacaccacg tcggtgcctg
     5581 ccgcgaccat ctcggtcacc ttcttcaacc actcgcgcgt ttcgtcgatc gagctgtgcg
     5641 ccgaccatgt caggtactgc gacagcgccg gatccgacac atacggaaag aaccgatccg
     5701 cgtcgctctc gacttgtggc cgcagcttca cgcgcggcgt ctcgatgacg agcggcagat
     5761 cggcgagctt cggaatcgga ttgcgaggtg gcgctgacaa catctcgcgc agaaccgtat
     5821 caggtaccgg cgaaattcct agctagtggg cgtcgtcggg atgcacttcc tcgacggtgg
     5881 cgaggatgtc cttcacgtcg atgatgcgca gtcgctcgtc ccacggcacg cgcacgccag
     5941 cgtccttcct gaacaacacg tgcgcgccga gcggcacgcc cgaaacgttc tcggtcgcct
     6001 cgtcggtcgt cggccgatcg cgcgccactt cgatcacctt gccgtagtgc gcgtcgtctt
     6061 gcgcttcctt cgcaccttgc ggcaggtaga gaccggcgtc ggtgcgatcc tcgtcgcgaa
     6121 tgagctgcac cagcacgcgc atcccgagtg gcaggatcag gcggtgcgtc ttcggcgggc
     6181 gggattcgtt ctcagaggtc acgagcggta acgacccggc cactctgctc caggcagcgt
     6241 aggctggtca agccggccgc cttgaacgag ggtgccacct cgtcgagggc cggtttcatc
     6301 cccgggtctt cgcacgatcc cgaacggacg tcgacgtgat cgccgatgat gctgacggtc
     6361 gaccacaggc gctgcttctt gagattacgc tcgaggcccg ccgcgacatc gagcgccgtc
     6421 gggccggtcg gttgcgcggg ttgctcggtc ggctgcgcgg gcgtctcgac ctcggcttgc
     6481 gccggtacgg ctggcgtctc gacggtggct gcaggttgcg ccgcgggctt ctcgttcttg
     6541 cgcagaccca aatacgcgat cgcgccgatc gcgacgacac cgatcgcgat cgcggtgatc
     6601 agcgggcctt tgccggtcgc gctcgcgact gccgtcccgc cctcgagatg cgagccccgc
     6661 gcgcggggca atgccttgtg gcatttcggg cagcggacat agccgggcac gaccgcgacg
     6721 ccgcacgacg ggcatacagc tccgctcatc ccttcaagga tacctggcta tcgccggcgc
     6781 cgccaaaaac caagcatcaa gaggcctgtc agcgcgagtg ggccggccgc gcctgagccg
     6841 gtgctgcagc aaccggtgtt gtcgtcgacg ggctcgcagg tccccggatc ctgggcggtg
     6901 ggatagatgc cgcagatgcc cgcgatgtcg tcgggttcga gcgtctcttt cttgttctcg
     6961 ccgcagtcct ggaagttgaa catcgtcgcg tcctgctgcg tcttggtcgc gtttccgcac
     7021 gacgggacag cgttgccttt gtcgtcgacg cgcggcgggt cgccggtggc gaggcacgtg
     7081 tgctcgaggc cgagcaagtg gccgagctcg tgggtgagcg tgttctggag ctcggagaga
     7141 cacgacgcgg tgccgagcgt cacgccgttg accgagatcg cgaagttctt gccgttgagc
     7201 tcgatatcgg cgtcgacgat cgcaccatcg cgcgagctgc ccgcatcgtc gacgtacgtc
     7261 gcggtcgtga tgcccgctgc ggagtcagga tggcagcgcg caggatcgtc ttgcgttgcg
     7321 ggccggcacc acgacgtgtc gcggaacttg atgaggttca cccggtcgcg accatcgacc
     7381 tcggcggctt tgcggccggc gttgatgatc tgcatgtacg agcacgacgc gacgccgttg
     7441 ttccaggtcg cgatcgcctg atcgatcacc ggaaattcgc cgtcaccggc gatctccttc
     7501 gtgcccgccg agtcgagcgt cacgaacacg caacccgatt cccagaacaa gtagtggccg
     7561 gccttcgtcg ggccggtgcg cacgtagttt gacgggtagg cgtcgacctt cacgccctcg
     7621 accgcgttgt gcatgcgctg ggacaggtcc atgctcgggc gcaccgcgac cgtgacccgc
     7681 attccgacgg cgagcggctc gtcgcccgga aactgccgca tcgtcaggcc gtcgaccgtg
     7741 ccgccgagct ggttgacgac gacgtcgccg ctagccgtgt ggacggtcgc ctcggtgacg
     7801 atctgcgcgc cgtccgccgt ccagtggctc cgaacgtcgc tgacgtcgcc ggaaaccgtc
     7861 attggcgctg cagttgcgct gcccgtcaga gccacaacca gcacgaagct acgcatctag
     7921 atgaggctat cacggcatgt tacacacgcc ccactatgag gccggtacgt gtgcgaattg
     7981 caccatcgcc gaccggcgat ccgcacgtcg gtactgcgta catcgcattg ttcaactacg
     8041 tgtttgcgaa gcagcagggc ggcaagttcg tcctgcgcat cgaggacacg gaccagaccc
     8101 gcgctcgcgc tgactccgag cagatgatct tcgacgcgct tcgctgggtc gggctcagct
     8161 gggacgaagg tccggatgtc ggcgggccat acggtccgta tcgccagagt gagcgcagcg
     8221 agctctatct gacgcacgtc gggatgctgc tcgagagcgg cgccgcttat cgctgcttct
     8281 gcacggccga gcgcctcgag aaggtgcgcg tccagcagca agccgagaag gcgacgctcg
     8341 ggtacgaccg gcactgccgc aatctcgatc cggacgaggc acgcaaccgc gcggcgcagg
     8401 gcgaagcgca cgtggtgcgg ctcaaggtgc cgctgtcggg gccgatcgag ttcacggaca
     8461 agttgcgcgg caaggtcacg cgcgacgcca aagagatcga cgatcaggtg ctgctcaagt
     8521 cagacggcct gccgacttat cacctcgcga acgtcgtcga cgatcacctg atggagatca
     8581 cgcatgtgat ccgcgccgag gagtggatca gctcgacgca gaagcacgtg ttgctctacc
     8641 gcgcgttcgg ctgggacgag cccgagttca tccacatgcc gctcttgcgt aacaaggaca
     8701 agagcaagat cagcaagcgc aagaacccgg tctcgatcaa ctactaccga gacatcggca
     8761 tcctgccgca cgcgctgctc aacttcctcg gcatgctcgg ctggtcgttc ggcggtgacc
     8821 gcgagaagtt cacgctgcaa gagatgatcg acgtgttctc gtgggaccgc atctcgctcg
     8881 gcggtccggt gttcgatctc gacaagctga cctggctcaa cgagaagtac atccacgacc
     8941 tgaactacga gcagttcgcc gacgcggtga tcgactggcg gctcaacaag gacttcctcg
     9001 tcaaggtgct gccgctcgtg cgcgagcgcg tgaagaagct cgcggacatc gttccggcga
     9061 ccgagtactt cttctcgggc gatctcgact acacggcggt gctccccgag ctgacgattc
     9121 ccggcgtcga agcgaaggtc gtcagcaagg gcgtgctcga tttcgtcgag cgcttcgagg
     9181 cgaaggatgg cttcgcgaag gaaatgctcg aggaggtcgc gcgcgcgtgg gccgaagcgc
     9241 agggctggaa gtccggccag gc
//
LOCUS       DASUFX010000198         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1836851, whole genome shotgun sequence.
ACCESSION   DASUFX010000198 DASUFX010000000
VERSION     DASUFX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836851"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFV99_07820"
     CDS             <1..346
                     /locus_tag="VFV99_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059251.1"
                     /translation="IEVPGSVEVGDAIMGEGHMLQGSGIGAMQIEVAEKPKTLDDAKS
                     DADMYSPKNLKDEKLADGWTLTFDNTGSMGKNFFVEVRRDINGKTYKCSTTTGDAKQA
                     AGALAACKTLRP"
     gene            complement(352..1266)
                     /locus_tag="VFV99_07825"
     CDS             complement(352..1266)
                     /locus_tag="VFV99_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5059252.1"
                     /translation="MLRLACAVLLVGCVAEMAPPGDESGSGTDEVPVAGVLPPSTASV
                     DEVVAWLAAELPARSPGTELSITFEDLQTGQRAVLSGDTKHVSASSAKAWWVAAALDG
                     AGLAAVAPYANAIFTNSDNSASGSVIDLIGPNAVNTWMWNVAGMNDSALTHWNYDKTR
                     VATNSPLALGDDNYMTSDDAVRFLGRVYRKEILADERGEQLLEWLTLSPNSGTGGWLL
                     ARLPAEVQDLGMHKGGWLPPGCCSDDGYYNTANEIGIVTTRFGRAYAVSILARRGTDY
                     WNRQVPFVELASCVMYRAFVQDDTLVCE"
     gene            1408..1893
                     /locus_tag="VFV99_07830"
     CDS             1408..1893
                     /locus_tag="VFV99_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059253.1"
                     /translation="MPDSGVIVQLPGEALPRLATAIAGLVPSNASRFMAALELGYLAA
                     SADGLDARERAALATTLEKVTGIGFDHDAFSAHFADLDDAVASLGRRERLARTAAEFE
                     TDDTREDAIRFAALVAMCDGKLNAQELEVLNEAGTHFRWSADKIHGLVTDAASRVRGG
                     K"
     gene            1890..2480
                     /locus_tag="VFV99_07835"
     CDS             1890..2480
                     /locus_tag="VFV99_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX5059254.1"
                     /translation="MSFVNRMSKEVNCKIVYYGAGLCGKTANLNYVFAATKPESRGKM
                     ISLETETERTLFFDFLPLDLGKIGEYKVRFHLYTVPGQVFYDASRKLILKGADGVVFV
                     ADSQEARLEANIESLENLEQNLGAYNGSLKQMPHVVQYNKRDMPSVSSIEELRSTLNL
                     HKAPEFEAVAKTGAGVFDTLKGLAKLVLNQLAKQGV"
     gene            complement(2477..3691)
                     /locus_tag="VFV99_07840"
     CDS             complement(2477..3691)
                     /locus_tag="VFV99_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059255.1"
                     /translation="MDPRLERAIGTPKIQQEAGDLVERSLRALEDLRQLDENLYERFL
                     ETRKTPEGPEAASNRIRKLWEETFKGLLTLLDYCQALAGGIPAPKTDDSDAFDFGDFG
                     ESAPEPAKDQLAIGAEDVSSALAGFFENPKDDGERWKPVLDKVGSIEYGLRSQYNEAV
                     ERRELSLSSGEMNQVLGILDDMTSTASEAVHALVAAVYEEFLPDVSPTSVVPGYLTTL
                     GRALLVRRGLADLSTKLAPLNDTLQSTQTDEHQHAFTQIRTIMRQFVGSVVCRAMRAA
                     DRWQMVEFERDLAGQSLLAGKQTSEGLVKYIESLSSVNQREVLVLHDQRTIVEMRDAV
                     AAARQLIDLSPRTARDEMERAYQAAQRLRGRHPATDALIIQLERFSSTSSHPGESSVF
                     LERLEATLSALS"
BASE COUNT          667 a         1218 c         1213 g          635 t
ORIGIN      
        1 gatcgaagtc cccggctctg tcgaagtcgg cgacgcgatc atgggcgaag gtcacatgct
       61 gcaaggctcg ggcatcggcg cgatgcagat cgaagtcgcg gagaagccga agacgctcga
      121 cgacgcaaag tccgacgcgg acatgtactc gccgaagaat ctcaaggacg agaagctcgc
      181 cgatggttgg acgctcacgt tcgacaacac cggcagcatg ggcaagaact tcttcgtcga
      241 agtgcgccgc gacatcaacg gcaagacgta caagtgctcg acgacgaccg gcgacgcaaa
      301 gcaagccgcg ggcgcgcttg ccgcgtgcaa gacgctgcgc ccttaaccta gctactcgca
      361 gacgagcgtg tcgtcttgca cgaacgctcg atacatcacg cacgacgcga gctcgacgaa
      421 cggaacttgc cggttccagt agtcggtgcc acggcgagcg agaatcgaga ccgcgtatgc
      481 tcggccgaac cgcgtcgtga cgatgccgat ctcgttcgcc gtgttgtagt agccgtcgtc
      541 gctgcagcag ccgggcggca accagccacc tttgtgcatg ccgaggtcct gcacctccgc
      601 gggcagccgc gcgagcaacc agccgccggt gccgctgttc ggcgacagcg tcagccactc
      661 gagcagctgc tcgccgcgct cgtctgcgag gatctccttg cgatagacgc ggccgaggaa
      721 gcggaccgca tcgtcgctcg tcatgtagtt gtcgtcgcca agcgcgagtg gcgagttcgt
      781 cgcgacgcgc gtcttgtcgt agttccagtg cgtgagcgcg ctgtcgttca tgccggcgac
      841 gttccacatc cacgtgttga ccgcgttcgg tccgatcaga tcgatcacgc tgccggatgc
      901 cgaattgtcg ctgttcgtga aaatcgcatt cgcgtacggc gcgaccgcgg cgagacccgc
      961 gccatcgagc gccgccgcca cccaccacgc tttcgccgag cttgccgaca cgtgcttggt
     1021 gtcgccgctg agcaccgcgc gctggccggt ctgcagatcc tcgaacgtga tcgacagctc
     1081 ggtccccggc gagcgagccg gcagctctgc cgcaagccag gcgacgacct catcgaccga
     1141 agcagtgctc ggtggcaaca cgccggcgac cggcacttcg tccgtgccgc tacccgactc
     1201 gtcaccgggc ggcgccattt cggcgacaca gccgacgagc agaacggcgc aagctaggcg
     1261 aagcacggcg gcggtccatg caatcgcgat gctgcgtgca gcccggcgaa atcacgtgtt
     1321 cccgtcggct gcacgcagtc cgcgtgtgca accgactgtg ggcaccagac ctttatggcg
     1381 ccgagcgttc gtgtgacgat tgtgtctatg cccgactccg gtgtgatcgt gcagctacca
     1441 ggggaggcgt tgccgcggct cgcgaccgcc atcgcgggac tcgtcccgtc caacgccagc
     1501 cgtttcatgg ccgcgctcga gctcggctac ctagccgcct cggccgacgg cctcgatgcc
     1561 cgcgaacgcg ccgcgctcgc caccacactc gaaaaggtga ccggcatcgg cttcgatcac
     1621 gatgcgttct cggcgcactt cgccgatctc gacgacgcgg tcgcatcgct cggtcgtcgc
     1681 gaacggctgg cgcgcaccgc cgccgagttc gaaactgacg acacccgcga agatgcgatc
     1741 cggttcgcag cgcttgtcgc gatgtgcgac ggcaagctca acgcacaaga gctggaggtg
     1801 ctcaacgaag cgggcacgca cttccgctgg agcgcagaca agatccacgg cctggtgacc
     1861 gacgcggcct cgcgcgtgcg aggtggcaaa tgagcttcgt caaccgcatg tccaaagagg
     1921 tcaactgcaa gatcgtctat tacggcgccg gtctctgcgg aaagaccgcg aacctcaact
     1981 acgtgttcgc ggcgacgaag ccggagtcgc gcggcaagat gatctcgcta gagaccgaaa
     2041 ccgagcgcac gctgttcttc gacttcctgc cgctcgatct cggcaagatc ggcgagtaca
     2101 aggttcgctt ccacctctac accgtgcccg gccaggtctt ctacgacgcg agccgcaagc
     2161 tgatcttgaa gggcgccgac ggtgtcgtgt tcgtcgctga ctcgcaagag gcgcgtctgg
     2221 aagcgaacat cgagagcctc gagaacctcg agcagaacct cggtgcctac aacggctcgc
     2281 tgaagcagat gccgcacgtc gtgcaataca acaagcgcga catgccgagc gtgtcgtcga
     2341 tcgaagagct gcgctcgacg ctcaatctgc acaaggcgcc cgagttcgaa gccgtcgcga
     2401 agaccggcgc cggcgtgttc gacacgctca aaggcctcgc gaagctcgtg ctgaaccagc
     2461 tcgcgaaaca gggcgtctag gagagcgcgg agagcgtcgc ctcgaggcgt tcgagaaaga
     2521 cgctgctctc gcccgggtgc gatgacgtcg acgagaagcg ctcgagctga atgatcagcg
     2581 cgtcggtcgc gggatggcgg ccgcggagtc gctgcgccgc ttggtacgcg cgctccatct
     2641 cgtcgcgggc ggtgcgcggg ctgagatcga tgagctggcg tgcggcggcg actgcatcgc
     2701 gcatctcgac gatcgtgcgt tgatcgtgga gtacgagcac ctcgcgttgg ttcaccgacg
     2761 acagcgactc gatgtatttg acgaggcctt cgctcgtctg cttgccggca agcagcgact
     2821 gaccggcgag atcgcgctcg aactcgacca tctgccagcg atcggcggcg cgcatcgcgc
     2881 gacacacgac cgaaccgacg aactgacgca tgatcgtgcg gatctgcgtg aacgcgtgct
     2941 ggtgctcgtc cgtctgcgtc gattgcagtg tgtcgttgag cggcgcgagc ttcgtcgaga
     3001 gatcggcgag gccgcgacgc acgagcagcg cacggccgag cgtcgtgaga tagccgggga
     3061 cgacgctcgt cgggctgacg tcggggagaa actcttcgta gaccgcagcg acgagcgcgt
     3121 gcacggcttc gctcgctgtg ctcgtcatgt cgtccaggat gccgagcacc tggttcatct
     3181 cgccggatga cagcgacagc tcgcggcgtt cgacggcctc gttgtattgc gagcgcaggc
     3241 cgtattcgat cgaaccgacc ttgtcgagca ccggcttcca gcgctcgccg tcgtctttgg
     3301 ggttctcgaa gaaaccggcc agcgcggagc tgacgtcttc ggcgccgatc gcgagctgat
     3361 ccttcgcggg ctcgggtgcg gattcaccga agtcgccgaa gtcgaacgcg tcggagtcgt
     3421 cggtcttcgg cgccgggatg ccgcccgcga gtgcctggca gtagtcgagc agcgtgagca
     3481 gacccttgaa cgtctcctcc cacagcttgc ggatgcggtt ggaggccgcc tccggacctt
     3541 ccggcgtctt acgcgtttcg aggaaccgct cgtagaggtt ctcatcgagc tggcgcagat
     3601 cctcgagcgc gcgcagggac cgctcgacca gatcacctgc ttcctgctgg atcttgggcg
     3661 taccgatcgc tcgttcgagt cgtgggtcca ccgctagctg agtgtcgcat gcctacggaa
     3721 atccggccac tta
//
LOCUS       DASUFX010000199         4404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_30441,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000199 DASUFX010000000
VERSION     DASUFX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30441"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1020)
                     /gene="gap"
                     /locus_tag="VFV99_07845"
     CDS             complement(13..1020)
                     /gene="gap"
                     /locus_tag="VFV99_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5059256.1"
                     /translation="MAKVRVGINGFGRIGRGFVRCLAGAKDAFDLVLINDLTDVGTLG
                     HLLRHDSVHGRFPGTVETKEGALVINGDTVKISAEKDPTAIKWKDLGVDVVIESTGKF
                     VDKAGAGKHIQGGAKKVIISAPAKDPDVTLCMGINLEMYKPGQHNIISNASCTTNCLA
                     PVAKVLQESFGIVSGIMTTIHSYTNDQQILDLPHKDLRRARAAALSMIPTTTGAAKAL
                     KEVLPAMAGKLDGMAIRVPTPNVSLVDLTARLEKSADAKTINEAFRKAAAGPLKGILA
                     VSDEPLVSCDYNGDRHSSTVDAALTTVVGDTVKVMSWYDNEMGYSQRLFDLARYVAER
                     L"
     gene            complement(1273..1563)
                     /locus_tag="VFV99_07850"
     CDS             complement(1273..1563)
                     /locus_tag="VFV99_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059257.1"
                     /translation="MRHYALESSVSKMYCLSLQGVVPLCAWHAPCSNAPLFLMNDREA
                     KARAVKILAKSIYRDLEAQGFDEKQIVALATELISEVTNKISRDHNKSHQLA"
     gene            1661..2110
                     /locus_tag="VFV99_07855"
     CDS             1661..2110
                     /locus_tag="VFV99_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5059258.1"
                     /translation="MSPNQIFVRHLVLTFTAIAAITTLALWLGAASRRENARPSAVAR
                     PEERPRSLLELGAHLYESKGCVACHTIDGSPRVGPSFKSSWGREVTLGDGVTLIFDET
                     YVRESLTYPQAKARPGYPPSMPSYDGLLTPREADALIAFMRSLASPR"
     gene            2225..2380
                     /locus_tag="VFV99_07860"
     CDS             2225..2380
                     /locus_tag="VFV99_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059259.1"
                     /translation="MSLGRRIVQRAATRVFRRGIRVVVRKQDEETPSAAESTATDNMP
                     VEASAAQ"
     gene            2488..4332
                     /locus_tag="VFV99_07865"
     CDS             2488..4332
                     /locus_tag="VFV99_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059260.1"
                     /translation="MIRRHVLAALDVGITAHDEDAARVALRRIDPIARGLARRTLERS
                     LIDAALACDAEQITAPPADHVMRLAALGLAKKPADVRDVAGTAAAYAQLPARKLSRVP
                     LLTIASVVFLAAFVGFGALYVQHALEKPSRTYAKKLPPPSADAYAKGGVPLTDPAVDK
                     LLTEELTKLVVEGGKAKNHEMNSLASVLDGLYAPDPFLKRGVALTKAWGHMLDVFNQS
                     TQVEVTEHANNELAESVRDFTAQLHAAGLGYVLEGRFKNGYPYIQAYRVVEIVFVTTA
                     GTPRRVLSIRRLDKLSSAYAVLGMHEEDLDPVLHLERIDENVATDVLPVLADGASYPL
                     ADREWMLTEPGKSLAAKVGDTVRREYKAALGNDAGTVDEIAKLLTKRNDIVDEWRDHL
                     LRKHIYFVRTENLFLPPNLLEQLEDVTPNYQRRRVREIEERLAEIEAPRIHARIHEMV
                     AATVRRHEAQHGFDYDRDTELRYPSQLQDFLGSPHDSSGNPVPVVRSARAELSAYLSQ
                     VANDPVTPQAAVWHLGAQVFNRDRRGTGEYYAGIVLIEGLARHLGAQPDGSRYVSREA
                     LAPLAIAIANAPADKLRAAAQALCTDLYGEPVTPIVDAPPRNVLAQAQ"
BASE COUNT          766 a         1449 c         1427 g          762 t
ORIGIN      
        1 ccggcagcga cattagagcc gctccgcgac gtagcgcgcg aggtcgaaca gccgctgcga
       61 gtagcccatc tcgttgtcgt accagctcat caccttgacg gtgtcgccga ccacggtggt
      121 cagcgcggca tcgacggtcg agctgtggcg atcgccgttg tagtcgcacg acacgagggg
      181 ctcgtcgctg accgcgagga tgcccttgag cgggccggcc gcggccttgc ggaacgcttc
      241 gttgatcgtc ttggcatcgg ccgacttctc gaggcgcgcc gtgagatcga cgagcgagac
      301 gttcggtgtc ggcacgcgaa tcgccatacc gtcgagcttg ccagccattg cggggagcac
      361 ttccttgagt gccttcgcag cgccggttgt cgtcggaatc atcgacagcg cagcggcgcg
      421 cgcacggcgc agatccttgt gcggcagatc gaggatctgc tggtcgttcg tgtacgagtg
      481 gatcgtcgtc atgatgccgc tgacgatgcc gaacgactcc tgcagcacct tggcgacggg
      541 cgcgaggcag ttcgtcgtgc acgacgcgtt cgagatgatg ttgtgctggc cgggcttgta
      601 catctcgagg ttgatgccca tgcacagcgt gacgtcagga tccttcgcgg gggcgctgat
      661 gatgaccttc ttcgcgccgc cttgaatgtg tttgccggcg ccggccttgt cgacgaactt
      721 gcccgtcgac tcgatcacga cgtcgacacc gaggtccttc cacttgatcg cggtcggatc
      781 cttctcggcg ctgatcttca ccgtgtcgcc attgatgacg agcgcgcctt ctttcgtctc
      841 cacggtgcct ggaaagcgac cgtgcaccga gtcgtggcgc agcaagtgac cgagcgtgcc
      901 gacgtccgtc aggtcattga tgagaacgag atcgaaggcg tcctttgccc cggcgagaca
      961 acgaacgaac ccgcggccga tgcggccgaa cccattgata ccgacgcgaa cctttgccat
     1021 ggcgcgcacc gtagatcatg gatgctcgca ctgccaggga cagtgacccg ttcgcgcgca
     1081 ggtcagtgca gcgaatgcgg tacgtcgaga aacgcaacgc gagcgcgggc gcacggccgc
     1141 actaccggat caccagcaga tccggcgcgg agtgcacgac cattcatgcg cggattgcga
     1201 ccgactacgc gagccaattt tggctgcggt ggccgcgagc ggcgccgagt caggtaccga
     1261 gccggtaccg tctcaggcga gttgatgcga cttgttgtgg tcgcgggaga tcttgttggt
     1321 cacttcggaa atcagctcgg tggcgagggc gacgatctgc ttttcgtcga acccctgcgc
     1381 ctcgagatcg cggtagatcg acttcgcgag gatctttacc gcacgggctt tggcttcacg
     1441 gtcattcata aggaacaagg gcgcgttaga gcacggcgcg tgccacgcgc acagcggtac
     1501 aactccctgt aaactcaggc agtacatttt actgacggac gattccagtg cgtaatgccg
     1561 cacttaggtg catatcgcgt tacgcacacg gtccgaccat cggatccatt cgcgacgatc
     1621 cgccgcaatt ccaccggctt actcggggca tctggtcggt atgtccccga accaaatctt
     1681 tgtccggcat ctcgtgctca cattcacggc catcgccgcg atcacgacgc tcgcgctttg
     1741 gttgggcgcc gccagcaggc gcgagaacgc acgaccatca gccgtcgcgc gaccggagga
     1801 acggccacgc tcgctgctcg agctcggcgc gcacctctac gagagcaagg gctgcgtcgc
     1861 gtgccacacg atcgatggct cgccgcgcgt cggtccgtcg ttcaagagca gctggggcag
     1921 ggaggtcacg ctcggtgacg gcgtgacgct gatcttcgac gagacgtacg tgcgcgagtc
     1981 gctcacgtat ccgcaggcaa aagcgcgccc cggctatccg ccatcgatgc cgtcgtacga
     2041 tggcctgctc acgccgcgcg aggccgacgc gctgatcgcg ttcatgcgtt cgcttgcctc
     2101 cccgaggtag cgacgtgtca accgcgtgcg gcgtgcgagt catgcaacgt cgcgatggtt
     2161 cgtcgatcac ctcgggtatg gctctagcaa tgcgcggtgg tgttcatcta caggagcacc
     2221 atccatgagt ctgggccgcc gcatcgtgca gcgcgccgcg acgcgagtct ttcgccgcgg
     2281 cattcgtgtc gtcgttcgca agcaagacga ggaaaccccg tcagccgccg aaagcacggc
     2341 gaccgacaac atgcccgtcg aggccagcgc ggcgcaataa ttcgtgcgac ctgcggtcgc
     2401 ggcgcggctc gtatctagtg aacccggtgc ggcgaaaaag tattcgttgc aacgggcgac
     2461 cctcgtacgt caaatccgcg cctagctatg attcgccgcc acgttctcgc tgccctcgac
     2521 gtaggtatca ccgcgcatga tgaagacgcc gcgcgtgtgg cgctgcggcg catcgatccg
     2581 atcgcgcgcg gacttgcgcg ccgcacgctc gagcgcagcc tgatcgatgc cgcactcgcg
     2641 tgcgatgcgg agcagatcac cgcgccgccg gccgatcacg tgatgcgact ggcggccctc
     2701 ggcctcgcga agaagccggc tgacgttcgc gatgtcgcgg gaaccgcggc cgcgtatgcg
     2761 cagctgccgg cgcggaagct gtcgcgcgtg ccgctcctga cgatcgcgag cgtggttttc
     2821 ctcgcggcgt tcgtcggctt cggggcgctc tacgtccagc acgcgctcga aaagccgagc
     2881 cgcacgtacg cgaagaagct gccgccgccg agcgccgacg catacgcgaa gggtggggtg
     2941 ccgctgaccg atccggcggt cgacaagctg ctcaccgagg agctgacgaa gctcgtcgtc
     3001 gaaggcggca aggccaagaa ccacgagatg aacagtctcg cgagcgtgct cgatggcctc
     3061 tacgcacccg atccgttctt gaagcgcggc gtcgccctga ccaaggcgtg gggacacatg
     3121 ctcgacgtgt tcaatcagtc gacgcaggtc gaggtgaccg agcacgcgaa caacgagctc
     3181 gccgagtcgg tgcgcgattt caccgcacag ctgcatgcgg ccgggctcgg gtacgtgctc
     3241 gaaggtcgct tcaagaacgg ctatccatac atccaggcct atcgcgtcgt cgagatcgta
     3301 ttcgtgacga ccgcgggcac gccgcgtcgc gtgctctcga tccgccgcct cgacaagctg
     3361 agctccgcgt acgcggtgct cggcatgcac gaagaggatc tcgatccggt gcttcatctc
     3421 gagcgcatcg acgagaacgt cgcgaccgac gtgctgccag tgctcgccga cggcgcgtcg
     3481 tatccgctcg cggatcgcga gtggatgctc accgagccgg ggaaatcgct cgccgcgaag
     3541 gtcggcgaca ccgtgcgccg cgagtacaag gcggcgctcg gcaatgatgc tggtaccgtc
     3601 gacgagatcg caaagctgct cacgaaacgc aacgacatcg tcgacgaatg gcgtgaccac
     3661 ctgctccgca agcacatcta cttcgtgcgc accgagaatc tgttcctgcc gccgaacctg
     3721 ctcgagcagc tcgaagacgt cacgccgaac tatcagcgcc gccgcgtgcg cgagatcgag
     3781 gagcgactcg ccgagatcga agcgccgcgc attcacgcgc gcattcacga gatggtcgcc
     3841 gcaacggtgc gtcgtcacga agcgcagcac ggcttcgact acgaccgtga caccgagctg
     3901 cgctacccga gccagctgca agatttcctc ggctcgccgc acgacagcag cggcaatccg
     3961 gttccggtcg tgcgcagcgc gcgggccgag ctatcggcgt acctgtcgca ggtcgcgaac
     4021 gatccagtga cgccgcaagc ggcggtgtgg catctcggcg cgcaggtgtt caaccgcgat
     4081 cgccgcggca ccggcgagta ctacgccggc atcgtgctga tcgaaggcct cgcgcggcac
     4141 ctcggtgcgc agcccgacgg ctcgcgctac gtgtcgcgtg aagcgcttgc gccactcgcg
     4201 atcgccatcg cgaatgcacc cgccgacaag ctgcgcgccg ccgcgcaggc gctgtgtacc
     4261 gacctctacg gcgagccggt gacgccgatc gtcgacgcgc cgccgcgcaa tgtgctagct
     4321 caagcacagt gataccgacg ctggtcgccg tcgtcgcgtg cctcgtactc gccgtgctca
     4381 gcgagctcgc gcggcgtggc gcag
//
LOCUS       DASUFX010000200         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1836909, whole genome shotgun sequence.
ACCESSION   DASUFX010000200 DASUFX010000000
VERSION     DASUFX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836909"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFV99_07870"
     CDS             complement(<1..1166)
                     /locus_tag="VFV99_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059261.1"
                     /translation="MRLICFVACLLFGLARAHADSPIRVAIECEQPGRTKACPAFLLG
                     FIDANKVLLNSPRAGADVVVYATATEVALIDRMHLRFVSRMPGAPEVIEIDVDLDSRA
                     NDDEQRAQLLPAFLRGLTLYVGARHPEAVTVSLNAPAGGGEADVTDGSPWSLVVELKG
                     NGSYTERYRSASGNLNLIGRWIMPKRRALAGVFSDAGINRQPPLTLDDGTVVSLDTNT
                     WAIHAGAEYIELLDEHWSTGIGSYTDFNDPKGQYAYSNRTRAAIEWDRFASNDPRGNR
                     LGVFYSLGWKTERYRVRNELGETFATYPVHGVTAFGSVRFDKITLGLSMDLEAQLLHP
                     MRRHSLTVSPQVSMKVGDHVDVGVTFSLTQRELPAPDMSAIDPSDYALISRLSYA"
     gene            1248..1784
                     /locus_tag="VFV99_07875"
     CDS             1248..1784
                     /locus_tag="VFV99_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059262.1"
                     /translation="MRLAALVLVGACGGASGTASVTATTSAAVSDAVTTNLTEGDLGP
                     SPDEVQDHLHQVAVYSADFDRLYVEVTSDGEHANVLRQSATTGLTAVPYIVSVDDGGE
                     LELRVEVAKLTPASDGTACGIKIFVMRLPQHDLLAIADGSGRATGKNATDTCLSATGT
                     AVVRQKLPPFLERQLDAK"
     gene            complement(1805..2458)
                     /locus_tag="VFV99_07880"
     CDS             complement(1805..2458)
                     /locus_tag="VFV99_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5059263.1"
                     /translation="MTITITAFERSPDGGKGLARDTRVRWALEEVGQPYEVRLVSFQA
                     MKQPAHLALHPFGAIPTYEEGDLALFETGSIVFHIAERYPGLLPADANARARAITWMF
                     AALSTIEPPILELANAVLLEADKPWHEQRLPLVKDRVRNRFNQLSARLGKADWLDGAF
                     SAGDLLMVSVLLRARRSRILDEYPNLAAYVARGEARPAYQRAFAAQLAVNSGKPASG"
     gene            complement(2476..>3330)
                     /locus_tag="VFV99_07885"
     CDS             complement(2476..>3330)
                     /locus_tag="VFV99_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059264.1"
                     /translation="AAPEHAAEVGSKLVGIGSHTIDEVIATLTPLISRDNDAGLHNEL
                     PFALADAGALAGVDLAAADHATFKLMAVDGREHDLELRSGDHAPKIAPPTPLPLQFSG
                     PSTNYWNKYIDADRLLYFGYNACAEDPRVGSFAGFVDSTLAFAEQHPVDRFVIDLRKN
                     GGGNSNIINPLLDGLAKRPNLARHVFVIIGMNTFSSAVLNAMELKRRLHATLVGGPTA
                     GSPSHFGEVKTFELPHSHLSVQYATKFFAYADFAGDAVQPDLPVKVTSADWFAGRDPA
                     LDAIRAAP"
BASE COUNT          606 a         1065 c         1091 g          568 t
ORIGIN      
        1 gcatacgaca gtcgcgagat cagcgcgtag tccgacggat cgatggcgct catgtcgggc
       61 gccggcagct cgcgctgggt cagcgagaac gtcactccga catcgacatg gtcgccgacc
      121 ttcatcgaga cctgcggtga caccgtcaga ctgtgccgcc gcatcgggtg cagcagctgt
      181 gcttcgagat ccatcgacag gcccaacgtg atcttgtcga atcgcaccga gccaaacgcg
      241 gtgacgccgt gtaccgggta cgtcgcgaac gtctcaccga gctcgttgcg cacgcggtag
      301 cgctccgtct tccaaccgag gctgtagaac acaccgagtc gattaccccg cggatcgttg
      361 ctcgcaaacc ggtcccactc gatcgccgcg cgcgtgcggt tcgagtaggc gtactgcccc
      421 ttggggtcgt tgaagtccgt gtagctgccg atgccggtcg accaatgctc gtcgagcagc
      481 tcgatgtact cggcgcccgc gtggatcgcc cacgtgttcg tgtcgagcga aacgacggta
      541 ccgtcgtcga gcgtcagcgg cggctggcgg tttatgcccg cgtcggaaaa cacgccggca
      601 agtgcgcgcc gctttggcat gatccagcgg ccgatcaagt tcaagtttcc ggatgccgat
      661 cgatagcgct cggtgtagct gccgttgccc ttgagctcca cgacgagcga ccaaggtgaa
      721 ccgtcggtga cgtcagcctc gccaccgccc gccggtgcgt tcagcgacac ggtgacagcc
      781 tcgggatgcc gcgcgccgac gtacaacgtg agaccgcgca agaatgccgg caacagctgg
      841 gcgcgttgct cgtcatcatt cgcacgcgag tcgagatcga cgtcgatctc gatgacctcc
      901 ggcgcacccg gcatccgact gacgaaccgc agatgcatgc ggtcgatcag cgcaacctcc
      961 gttgccgtcg cgtacaccac gacatcggcg cctgcgcgcg gcgaattcag gaggaccttg
     1021 ttcgcgtcga tgaagccgag caagaacgcg gggcacgcct tggtgcggcc gggttgttcg
     1081 cactcgatgg cgacgcggat cggcgagtcg gcgtgcgccc gcgcgagccc aaacaacagg
     1141 caagcgacga aacaaatcaa tcgcacggac gcgagagtac atcggtcgtg tcgcgccgcg
     1201 aggtcgacgt gtggattatc ggccgcagca cggttaccgt gtgatccatg cgactcgcgg
     1261 ccctcgtttt ggtcggcgcg tgcggcgggg ccagtggcac cgcttccgtc accgccacga
     1321 ccagcgcggc ggtcagcgac gcagtcacca cgaatctcac cgagggcgac ctcggccctt
     1381 cgcccgacga agtgcaggat catctccatc aggtcgcggt gtactccgcc gattttgaca
     1441 ggctctatgt cgaggtcacg agcgacggcg agcacgcaaa cgtactgcgc cagagcgcga
     1501 cgaccggtct caccgccgtg ccgtacattg tctcggtcga tgacggcggc gagctcgagc
     1561 tccgtgtcga ggtcgcgaaa ctcacgccgg cgagcgacgg cacggcctgc ggcatcaaga
     1621 tcttcgtgat gcggctgcca cagcacgacc tgctcgcgat cgccgacggc tccggacgtg
     1681 ccaccggcaa gaatgcgacc gatacgtgtc tttcagcgac cgggacagcc gtcgtgcgac
     1741 agaagctacc gccgtttctc gagcgccaac tcgacgcgaa gtgatcgaac cgaacggacg
     1801 gtgcttagcc actcgccggc ttgccgctgt tgaccgccaa ttgagcggcg aaggctcgct
     1861 ggtacgcggg ccgcgcttcg ccacgtgcga catacgcggc gaggttcgga tactcgtcga
     1921 gaattctcga ccgtctcgcc ctgagcagca ccgacaccat cagcaagtcg cccgcgctga
     1981 acgcaccgtc gagccagtcg gccttgccaa ggcgcgccga gagttggttg aaccgattgc
     2041 gcacgcggtc cttgaccaac ggcaggcgct gctcgtgcca cggcttgtcg gcctcgagga
     2101 ggacggcatt tgcgagctcg aggatcggtg gctcgatcgt gctgagcgcg gcaaacatcc
     2161 atgtgatcgc gcgcgcccgc gcattggcat ccgccggcag caagccggga taccgctcgg
     2221 cgatgtgaaa cacgatcgac ccggtctcga atagcgcgag atcgccttcc tcgtaggtcg
     2281 gaatcgcgcc aaacggatga agcgccagat gcgcgggttg tttcattgcc tgaaaggaaa
     2341 ccaggcgaac ctcgtagggt tggcccactt cttcgagagc ccagcgaacg cgcgtatcgc
     2401 gcgcgagtcc cttgccgcca tcgggtgagc gttcaaacgc ggtaatggtg atcgtcatcg
     2461 agagctcctt gttggctacg gcgccgcgcg aatcgcgtcg agcgccgggt cgcggccggc
     2521 gaaccagtcg gccgaggtca ccttcacggg aaggtcgggc tgtaccgcgt cgccggcgaa
     2581 atcggcatac gcgaagaact tggtcgcgta ctgcaccgat aggtgcgagt gcggaagctc
     2641 gaaggtcttc acttcgccga aatgcgatgg actccctgca gtcgggccgc cgacgagcgt
     2701 tgcgtggaga cggcgcttca gctccatcgc gttcagcact gccgacgaga acgtgttcat
     2761 gccgatgatc acgaacacgt ggcgggcgag gttcgggcgc ttcgcgaggc catcgagaag
     2821 tggattgatg atgttggagt tgccgccgcc gttcttgcgc aaatcgatca cgaagcgatc
     2881 gacggggtgt tgttccgcga acgccagcgt cgaatcgacg aagcccgcga acgagccgac
     2941 gcgcgggtcc tccgcacacg cgttgtaacc gaagtacagc aagcggtcgg catcgatgta
     3001 cttgttccag tagttcgtgc tcggaccaga gaactgcaac ggcagcggcg ttggtggtgc
     3061 gatcttcggc gcgtggtcgc cactacgaag ctcgagatca tgctcgcggc cgtcgacggc
     3121 catcagcttg aacgtcgcgt gatcggcagc cgcgagatcg acgccggcga gcgcaccggc
     3181 atcggcgagc gcaaacggaa gctcgttgtg gagacccgca tcgttgtcgc gcgagatgag
     3241 cggcgtgagc gtcgcgatca cttcgtcgat cgtgtggctg ccaatgccga cgagcttgct
     3301 gccaacttcg gccgcgtgct cgggcgcggc
//
LOCUS       DASUFX010000201         6809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_308548,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000201 DASUFX010000000
VERSION     DASUFX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308548"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFV99_07890"
     CDS             <1..384
                     /locus_tag="VFV99_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059265.1"
                     /translation="DPGYAVDRAVLGAAIDVFPPAPAKLGPAGAFRGEDTPLTDAGVV
                     LVRLPVRTPYSIVLEEQKYLAGAKGVRAATLRRLSPNGWVIGVATNESIEQVARIAKK
                     APMTDTMTTVKVVGDIVELSLAGAP"
     gene            381..692
                     /locus_tag="VFV99_07895"
     CDS             381..692
                     /locus_tag="VFV99_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059266.1"
                     /translation="MTRVLVVVSLVTALAACGGRQKPHQVDADDAIVVIRSNVTDANV
                     FVDGRYYGSVRMLRGGLAFEAGKHRLELRHDEYFSRYVELDLKRAEHKQLDLELAPVL
                     P"
     gene            complement(693..1370)
                     /locus_tag="VFV99_07900"
     CDS             complement(693..1370)
                     /locus_tag="VFV99_07900"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5059267.1"
                     /translation="MKRLTLRAFAGLAFLLAVVGLVLFGAAGTIGYWQAWVFLTVFGG
                     AAVLITIDLAVHDPALLERRVHAGPAAEQKTSQKVIQSLASLAFLATFVLAGLDHRAG
                     WSHIATPLAVAGNVVAAVGLYIVFRVFRANTFTSATIEVAREQQVVTTGPYAIVRHPM
                     YAGALIMMVGVPLALGSWWAVPAVLVLIGVIVWRLLDEENLLATELRGYAEYRTKVKY
                     RLVPLVW"
     gene            1422..2099
                     /locus_tag="VFV99_07905"
     CDS             1422..2099
                     /locus_tag="VFV99_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEX5059268.1"
                     /translation="MHARALLKDGFRVGQAVVRVLGDSTKTHEIHRVEEITSQGRMRA
                     LMRELAQTPEGRRLMDERPELSSEHIDYDRLRALPATTFGGAYARHLDNNGITADYQA
                     AATRHVDDPDIAYLVRRYRQTHDVWHALLNLGIAGHEEVLVHSFAYGQLHLPVSALVI
                     LFGGIKHIVLERRWGALRHSMYEAYRAGRAAAPLMAVYWEDLWEAPLETVRVAYNVEP
                     LERRWLD"
     gene            2109..2489
                     /locus_tag="VFV99_07910"
     CDS             2109..2489
                     /locus_tag="VFV99_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059269.1"
                     /translation="MEPRIETEQALRTVLDHITFAPSCVDMGWAWQIEELRLADGKLR
                     GWLVNTTFRRPDTHTGEIGIGSGRQELVGFGASESAVVKTAWLLAELIVRHELMEAFL
                     YKGVRIFNPHHTVEELSLPSRMKS"
     gene            2505..3839
                     /locus_tag="VFV99_07915"
     CDS             2505..3839
                     /locus_tag="VFV99_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5059270.1"
                     /translation="MTTSGSPQPSGSPQTAQVITLLRWALIIIFVAFSWIVLKYVARV
                     LAPVLAALGIAYLLNPVLERLVRAGVSRGVGAAFLLISFLGLIIGGIIAAVPAIGNQI
                     NDFAHDLPRLMTNLDLWLKSHFGMELPHDWKQYLNKENIEHTLGAGAVQQIATAALGG
                     AFALLGVMAEFLLVPVFAFYFLVDFPNLLRRIDHMIPPRRRSKVREILREIDRVIAGW
                     VRGQGIVTSILAVLYAIGFSIVGMKLAVPIGLLVGALTVIPFVGTFVGAGIAATVLVA
                     DGASIQTLGEVGGIFLVLHLLEAGFLTPKIVGHRVGLSETGALFAVVAGGKLLGFVGV
                     VLAVPIAATCAVLVRYAVRYYEHTEFFGRESDADIVITPAMAMIMPGVRIDTTRFTSE
                     AVEDWAIEPELGEAGMSVREMKPEAEPAPAPEPAAVLPEAPPAAAPPKKEEP"
     gene            3836..4615
                     /locus_tag="VFV99_07920"
     CDS             3836..4615
                     /locus_tag="VFV99_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5059271.1"
                     /translation="MSDLLVVDRGPVRTITLDRPDSKNGLTYEIAMQITAAITGGTAA
                     NVIVLAGANGAFCSGLDLKDAMQRGLKSGAELRQSMSESFHAVIRALRDSGVPTIAAV
                     DGVAVGFGCDLALACDIRICSDRASFGELFIKRGLMPDGGSTFLLPRIVGLGRALELF
                     YTGDSVDAQEALRIGLANKVYPAAELMDRVAELADRFAAGPPLAYEALKEAVYANLDE
                     SSLSRALDREADGQVKLLASKDFIEGVTAFLQKRAPKFQGH"
     gene            5174..5246
                     /locus_tag="VFV99_07925"
     tRNA            5174..5246
                     /locus_tag="VFV99_07925"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5207..5209,aa:Tyr,seq:gta)
     gene            5487..>6809
                     /locus_tag="VFV99_07930"
     CDS             5487..>6809
                     /locus_tag="VFV99_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059272.1"
                     /translation="MTARTLLPESQIGPAERLLDVVLGSSAHLWHNRPGMDVNGTWVP
                     AKNKTSKARGKKVAPGLFVPAAVALYTRLLEIHKLNADLMAHLASYALVETDWRDLKV
                     ACAALMLVQTRAGAPIHDDDGSVAFYDDDHRRIGEAMVLWYQKKSARMLTPKAVLRIA
                     ELLETPEIAALNRAAGFGDPAAKRAPLGRWSKAAGKWLSVRETNKAMLEGLVKAGYKQ
                     TIKNLARKIGYKPESPEFFGLLGWKQKQAEDGRREVGLDNTKLVKSERFDGLAEAEIC
                     ETIVSQKLRYKDAVGRLPADIGLTPAIMVALLPTLSDRDLRQLTPTLEELGLLSVPEI
                     RGRWEKAIESATDQRGLNIAKNVRDAALKEKLAESADNAAKKAVAAATAEVDLRVMFL
                     VDKSGSMQGAIETSKDILGKMLAGFPLEKLHVAAFDTMGQVLKPKAASS"
BASE COUNT         1175 a         2237 c         2209 g         1188 t
ORIGIN      
        1 gatccgggct acgcggtcga ccgcgcggtg ctcggcgccg cgatcgacgt gttcccgccg
       61 gcgccggcga agctcggtcc tgcgggtgcg tttcgcggcg aggacacgcc actcaccgac
      121 gctggcgtcg tgctcgtgcg gttgccggtg cgcacgccat attcgatcgt gctcgaggaa
      181 cagaagtatc tcgccggcgc caagggcgtc cgcgctgcga cgctacgccg gctatcgccg
      241 aacggctggg tgatcggtgt cgcgacgaac gaatcgatcg aacaggtcgc acgcattgcg
      301 aagaaggcgc cgatgaccga cacgatgacg acggtgaagg tcgtcggtga catcgtcgag
      361 ctctcgctcg caggagcgcc atgactcgcg tgctcgtggt cgtgtcgctc gtcaccgcgc
      421 tcgctgcgtg cggcgggcgg cagaagccgc accaggtcga cgcggacgac gcgatcgtcg
      481 tgatcaggag caacgtcacc gacgcgaacg tcttcgtgga tggccggtac tacggttcgg
      541 tgcgcatgct gcgcggtggg ctcgcgttcg aagccggcaa gcatcgccta gagctacgcc
      601 acgacgagta cttctcgcgc tatgtcgagc tcgacctcaa gcgcgccgag cacaagcagc
      661 tcgatctcga gctggcgccg gtactgccgt agctaccaga ccagcggcac gagccggtac
      721 ttcaccttcg tgcggtattc ggcgtacccg cgtagctcgg tcgcgagcag gttctcctcg
      781 tcgagcaggc gccatacgat cacgccgatg aggacgagga ccgcgggcac ggcccaccac
      841 gagccgagcg cgagcggtac gccgaccatc atgatcaacg cgcccgcgta catcgggtgg
      901 cgaacgatcg cgtacggccc ggtggtcacg acctgttgtt cgcgcgcgac ttcgatcgtt
      961 gccgacgtga atgtattcgc gcggaacacg cggaacacga tgtagaggcc gacggccgcc
     1021 acgacgttgc cggcgactgc gagcggtgtc gctatgtgcg accagcccgc gcggtgatcg
     1081 aggccggcca gcacgaacgt ggcgagaaac gcgagcgagg cgaggctctg gatcaccttc
     1141 tgactcgtct tctgctcggc ggctggcccg gcatggacgc ggcgctcgag caacgccgga
     1201 tcgtggaccg cgaggtcgat cgtgatcagg acggcagcac cgccgaagac ggtgaggaac
     1261 acccacgcct gccagtaccc gatcgtgccg gcggccccga acagcacgag cccgacgacg
     1321 gcgagcagaa acgcgagccc ggcaaacgcg cgtagcgtca ggcgcttcat gaacagaact
     1381 ctaccccgtg ctgaaaacgc ggcactgtgt acacttcggg catgcacgca cgtgcgctgc
     1441 tcaaggatgg tttccgcgtc ggtcaggcgg tcgtgcgcgt gctcggcgat tcgacgaaga
     1501 cgcacgagat ccatcgcgtc gaagagatca cgagccaggg ccgcatgcgc gcgctgatgc
     1561 gcgagctcgc gcagacgccg gaaggccggc gtctcatgga cgaacgcccc gagctgtcga
     1621 gcgagcacat cgactacgat cggctgcgcg cgctgccagc gacgacgttc ggcggcgcat
     1681 acgcgcgtca cctcgacaac aacggcatca cggctgacta ccaggcggcg gcgacgcgcc
     1741 acgtcgacga tccggatatc gcgtatctgg tccggcgcta ccgacagact cacgacgtct
     1801 ggcacgcgct cttgaacctc ggcatcgccg gccacgaaga agtgctggtc cactcgttcg
     1861 cgtacggtca gctccacttg ccggtgtccg cgctcgtgat cctgttcggc gggatcaagc
     1921 acatcgtgct cgagcgccgg tggggcgcac tccgccactc gatgtacgag gcctaccgcg
     1981 ccggccgcgc ggcagcgcca ctgatggcgg tgtactggga agatctgtgg gaagcgccgc
     2041 tcgagaccgt gcgcgttgcc tacaatgtcg agccgctgga acgccgctgg ctcgactaag
     2101 ctgcgtggat ggaaccgcgc atagagacag aacaggcgct gcgcactgtt ctcgatcaca
     2161 tcacgttcgc gccgtcgtgc gtcgacatgg gctgggcctg gcagatcgaa gagctgcggc
     2221 tcgccgacgg taagctgcgg ggctggctcg tcaatacgac gttccggcgc cccgacacgc
     2281 acaccgggga gatcgggatc ggcagcggcc ggcaggagct cgtcgggttt ggcgccagcg
     2341 agtccgccgt cgtgaagacc gcctggctcc tcgccgagct gatcgttcgc cacgagctga
     2401 tggaggcatt cctctacaag ggcgtgcgga tcttcaaccc gcaccacacc gtcgaggagc
     2461 tgtcgctgcc gagccgcatg aaatcgtgat acagcgttgg tggggtgacg acttcgggct
     2521 cgccacaacc ttcgggctcg ccgcagacag cgcaggtcat tacgctgctg cgctgggcgc
     2581 tgatcatcat cttcgtcgcg ttctcgtgga tcgttctcaa gtacgtcgcg agggtgctcg
     2641 cgccggtcct cgccgcgctc ggtatcgcgt atctgctcaa cccggtcctc gagcggcttg
     2701 ttcgcgccgg cgtctcgcgc ggcgtcggtg ccgcgttcct cttgatctcg ttcctcggcc
     2761 tcatcatcgg cggcatcatc gcggcggtcc cggccatcgg aaatcagatc aacgatttcg
     2821 cgcacgacct gccgcgcctc atgaccaacc tcgatctctg gctgaagagc cacttcggga
     2881 tggagctgcc gcacgactgg aagcagtacc tcaacaagga aaacatcgag cacacgctcg
     2941 gtgccggcgc cgtgcagcag atcgcgaccg cagcgctcgg tggcgcgttc gcgctcctcg
     3001 gcgtcatggc ggagttcctg ctcgtgccgg tgttcgcgtt ctacttcctc gtcgactttc
     3061 caaatctgct ccgccgcatc gaccacatga tcccgccgcg caggcgcagc aaggtccgcg
     3121 agatcctgcg cgagatcgat cgcgtgatcg ccggctgggt acgcgggcag ggcatcgtca
     3181 cgtcgatcct cgcggtcctc tacgcgatcg gcttctcgat cgtcggcatg aagctggccg
     3241 tgccgatcgg tctgcttgtc ggcgcgctca ccgtgatccc gttcgtcggc acgttcgtcg
     3301 gcgccggcat cgccgcgacc gtcctcgtcg ccgatggtgc gagcatccag acgctcggcg
     3361 aggtcggcgg catcttcctc gtgctccacc tgctcgaggc gggcttcctc acgccgaaga
     3421 tcgtcggtca ccgcgtcggc ctctcggaga ctggcgcgtt gttcgccgtc gttgccggcg
     3481 gcaagctgct cggcttcgtc ggtgtcgtgc tcgccgtgcc gatcgccgcg acatgcgcgg
     3541 tgctcgtgcg ctacgccgtc cgctactacg agcacaccga gttcttcggc cgcgagtccg
     3601 acgccgacat cgtgatcaca ccggcgatgg cgatgatcat gcccggcgta cgcatcgaca
     3661 ccacgcggtt cacgtcggaa gcggttgaag actgggcgat cgagcccgag cttggcgaag
     3721 cgggaatgtc ggtgcgcgag atgaagccgg aggccgaacc tgcacccgca cccgaaccgg
     3781 cggcggttct tcccgaagcg ccaccggctg cagcgccacc gaagaaggaa gagccatgag
     3841 tgacctcctc gtcgtcgatc gtggcccggt tcgcaccatc acgctcgatc gtcccgactc
     3901 gaagaacggc ctgacgtacg agatcgcgat gcagataacc gccgcgatca ccggcggcac
     3961 cgctgcgaac gtgatcgtgc tcgccggcgc gaacggtgcc ttctgctccg gcctcgatct
     4021 caaggacgcg atgcagcgcg gcctgaagtc cggcgccgag ctgcgccagt caatgagcga
     4081 atcgtttcac gccgtgattc gcgcgctgcg cgacagcggt gtgccgacga tcgcggcggt
     4141 cgatggcgtg gcggtcggtt tcggttgtga cctcgcgctc gcgtgcgaca ttcgcatctg
     4201 cagcgatcgc gcgtcgttcg gcgagctgtt catcaagcgc ggcctgatgc ccgacggcgg
     4261 cagcacgttt ctgttgccgc gcatcgtcgg tctcggccgc gcgctcgagc tgttctacac
     4321 cggcgattcc gtcgacgcgc aggaggcgct gcgcatcggt ctcgcgaaca aggtgtatcc
     4381 agcggccgag ctgatggatc gcgtcgccga gctcgccgat cgcttcgccg cgggcccgcc
     4441 gctcgcctac gaggcgctca aggaggcggt ctacgcaaac ctcgacgagt cttcgctgtc
     4501 acgcgcgctc gatcgcgagg ccgacggtca ggtgaagttg ctcgcgagca aggacttcat
     4561 cgaaggcgtc acggcgttcc tgcagaagcg ggcgccgaag ttccaaggtc actgactccc
     4621 gcgctcgcga tcgctctccg attgagctcg cgcgacgcgg agttatcgac aagtcgaaaa
     4681 aatgatctcg acaaccgact cgcgatcctt atagtgcatc tccatcaaat gccctcgacg
     4741 gtgaaacaac tcaacccatc ccactaacgg gtggctacgt cttccgtcga aggccgcctc
     4801 ccaaggcggc atcttttttt cggtcgtttc ggtttttaca gcttcggatc ccgccgcgcg
     4861 gtccctacac gcttccatga aaatggattc caagaggaaa cctcgttgga ccacgcactc
     4921 ggatctgtcg ctgaatgttt tcgctcggac gccccgccgt agaagcccta cacgcaccac
     4981 acctcggttc gattccaggt gaccccacca cacggcgact cacgtcgcct tatggggtca
     5041 agccgttgga cggcacttct ccttacaaga gaaatcttgg cggtttccac actcgggcgt
     5101 acgagcaatt tttcgccgcg atatccgcgg cacctgtttt cagcgtgtcc agccgagcag
     5161 agcccacacg cactctggaa tagctcattc ggtagagcat cagattgtag atctgagtgt
     5221 ggcgggttcg aaccccgctt ccaaaaccct cgcgctccgt tcactcggac acgctgaatc
     5281 tttttcgcgc ggccgttcgc acgccaccgc gccgttgttt ttcgcgccca gccgaacaag
     5341 ccctacacgc aagggttatg caggttcgaa tcctgctaag ggctcacgcc cttatcgcct
     5401 aatggcaagg cactccgctc ttaacggaaa ttgcggcttg ttcactcggg cgcgtttttc
     5461 ttttcggtaa ccttggagtc cttacgatga cggcacgcac gctacttccc gagtcgcaga
     5521 tcggtccggc agagcgcctg ctcgatgtcg tgctcggttc gagcgcacac ctctggcaca
     5581 accgcccggg catggacgtc aacggcacgt gggtgccggc gaagaacaag acgtcgaagg
     5641 cgcgcggcaa gaaggtcgcg ccgggtctgt tcgtgccggc ggctgtggcg ctgtacacgc
     5701 gcctgctcga gatccacaag ctcaacgccg acctgatggc tcacctcgcg agctacgcgc
     5761 tcgtcgagac ggactggcgt gacctcaagg tcgcgtgcgc cgcgctgatg ctcgtgcaga
     5821 cgcgcgcggg tgcgccgatc cacgacgacg acggctcggt cgcgttctac gacgacgacc
     5881 accgccgcat cggcgaggcc atggttcttt ggtaccagaa gaagagtgcg cgcatgctca
     5941 cgccgaaggc ggtgctgcgt atcgccgagc tgctggagac gccggagatc gctgcgctga
     6001 accgcgctgc gggctttggc gatccggccg caaagcgtgc tccgctcggt cgctggtcga
     6061 aggccgctgg caagtggctg tcggttcgcg agacgaacaa ggcaatgctc gagggcctcg
     6121 tgaaggctgg ctacaagcag acgatcaaga acctcgcgcg caagatcggc tacaagccgg
     6181 agtcgccgga gttcttcggt ctgctcggct ggaagcagaa gcaggccgag gacggtcgtc
     6241 gcgaggttgg tctcgacaac acgaagctcg tgaagagcga gcgcttcgac ggtctggccg
     6301 aggcagagat ctgcgagacg atcgtgtcgc agaagctgcg ctacaaggat gcggtcggtc
     6361 gcctgccggc agacatcggt ctcacgccgg cgatcatggt cgcgctgctg ccgacgctgt
     6421 cggatcgtga cctgcgccag ctgacaccga cgctcgagga gctcggcctg ctctcggtcc
     6481 cggagatccg gggtcgctgg gagaaggcga tcgagtcggc gacggatcag cgtggtctca
     6541 acatcgcgaa gaacgtgcgc gatgcagcac tcaaggagaa gctcgccgag agcgccgaca
     6601 acgcggccaa gaaggccgtc gcggcggcaa cggcggaggt cgatctgcgg gtgatgttcc
     6661 tcgtcgacaa gtcgggctcc atgcagggcg cgatcgagac gagcaaggac atcctcggca
     6721 agatgctggc cggtttccca ctcgagaagc tccacgtcgc agcgttcgat acgatgggcc
     6781 aggtcctcaa gccaaaggca gcgtcgagc
//
LOCUS       DASUFX010000202         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_30923,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000202 DASUFX010000000
VERSION     DASUFX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30923"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFV99_07935"
     CDS             <1..1118
                     /locus_tag="VFV99_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HEX5059273.1"
                     /translation="PNTPPKRWTQVVVYRGLAPRAAIASLRKADGSPLDYNPIDSYTM
                     DVIKNDYAFAMTYWVRGGELLNIALMYYEPNAAEFEREDWGDWFPIDHAEMQRSMSRA
                     FGDDKRKHDVLALASVMENPTKWGLYDRDAFEHWQDGRVCLLGDAAHPMLPTMGQGAS
                     QSFEDGAALSSCFKLHGTDVYSALLHYERVRHYRATRFQVASKFLFKHLEPPDSPERR
                     MLLKALNERDFPIFDHEQRAGDDDSWIYAFDARNVGDKLPQKKLGPWDFRARSAALDT
                     RKEIATNLWKPAVSLKGDRKVTRAELARHATFADCWIVIRGKVYDFTEWKDRHPGGPF
                     VARMYAGKDATAEFGDFHSKLAEKHMAHFCVGDLVDQ"
     gene            complement(1126..1737)
                     /gene="pssA"
                     /locus_tag="VFV99_07940"
     CDS             complement(1126..1737)
                     /gene="pssA"
                     /locus_tag="VFV99_07940"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX5059274.1"
                     /translation="MQKRFSMIREFHFADWFTLANAVSGMGAVLASMTYLETRAVTHV
                     YLACTAVLAALVFDVLDGRIARWRQSSSALGRELDSLADVISFGVAPAALAYACGMQG
                     FYDRVILAYFVACGVSRLARYNVTADALSGGSGKVAYFEGTPIPTSIVLVGMLALAAW
                     QQAIGDQLWLGEIEVAGFRLHWLTLAFAASGSLMISRIRIPKF"
     gene            1865..>2266
                     /locus_tag="VFV99_07945"
     CDS             1865..>2266
                     /locus_tag="VFV99_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5059275.1"
                     /translation="MAKQLEGKRIAFLATDGFEQSELEQPWNEIKNAGATVELLSVHK
                     GSIQGMRHMDKGDLFEVDGLVAEADAADYDGLVLPGGVANPDTLRTNLPAVEFVRDFF
                     AQAKPVAAICHGPWTLVEADAVRGRTVTSWPS"
BASE COUNT          446 a          751 c          737 g          332 t
ORIGIN      
        1 ggccgaacac tccaccgaag cgctggacgc aggtcgtcgt gtaccgcggg ctcgcgccgc
       61 gcgccgcgat cgcgagcttg cgcaaggcgg acggcagtcc gctcgactac aacccgatcg
      121 attcgtacac gatggacgtc atcaagaacg actacgcgtt cgcgatgacg tactgggtgc
      181 gcggcggcga gctcctcaac atcgcgctga tgtactacga gccgaacgcc gccgagttcg
      241 agcgcgaaga ctggggcgac tggttcccga tcgatcacgc cgagatgcag cgctcgatga
      301 gccgcgcgtt cggtgacgac aagcgcaagc acgacgtact cgcgctcgcg agcgtcatgg
      361 agaacccaac gaagtggggc ctatacgatc gcgacgcgtt cgagcactgg caagacgggc
      421 gggtgtgcct gctcggggac gcggcgcacc cgatgctccc gacgatgggc caaggcgcgt
      481 cgcagtcgtt cgaggacggc gccgccctct cgagctgctt caagctgcac ggcacggacg
      541 tctacagcgc cctgctgcac tacgagcgcg tgcgccatta ccgcgcgacg cgctttcagg
      601 tcgcgtcgaa gtttttgttc aagcacctcg agccgcccga ttcaccggag cggcggatgc
      661 tgttgaaggc gctgaacgaa cgcgactttc cgatcttcga tcacgagcag cgcgccggcg
      721 acgacgactc gtggatctac gcgttcgatg cgcgcaacgt cggcgacaag ctgccacaga
      781 agaagctggg cccgtgggac ttccgcgcgc gcagcgcggc gctcgacact cgcaaggaga
      841 tcgcgacgaa tctctggaag ccggccgtgt cgttgaaggg cgatcgtaaa gtcacgcgcg
      901 cggagctcgc gcgccacgcg acgttcgcgg actgctggat cgtcatccgc ggcaaggttt
      961 acgacttcac ggagtggaag gaccgccatc ccgggggccc gttcgtcgca cgcatgtatg
     1021 cgggcaaaga cgcgacggcg gaattcggcg acttccacag taagcttgcc gagaagcaca
     1081 tggcgcactt ctgcgtcggc gacctcgtgg accagtagcg gctcgctaga acttcgggat
     1141 ccggatacgg ctgatcatca gcgagccgga cgccgcgaac gcgagcgtca gccagtgcag
     1201 cctgaagccg gcgacctcga tctcgccgag ccacaactga tcgccgatcg cttgttgcca
     1261 agcggccagc gccaacatgc cgacgagcac gatcgacgtc gggatcggcg tgccctcgaa
     1321 atacgcgacc ttgcccgatc cgccggacaa cgcgtccgcc gtgacgttgt agcgcgcgag
     1381 acgcgacacg ccgcatgcga cgaagtacgc gaggatcaca cggtcgtaga acccctgcat
     1441 gccgcacgcg tacgcgagcg cggccggcgc aacaccgaac gaaatcacgt cggccaacga
     1501 gtcgagctcg cggcctaggg ccgacgacga ctgtcgccag cgcgcgatgc gcccgtcgag
     1561 cacgtcgaag acgagcgcgg cgagcaccgc ggtgcacgcg aggtacacat gcgtcacggc
     1621 gcgcgtctcg agatacgtca tgctcgcaag cacggcgccc attccggaga ccgcgttcgc
     1681 aagcgtgaac cagtccgcga agtgaaactc gcgaatcatc gagaagcgct tctgcatgga
     1741 cacacgctac cacagcggcg gcaaagccca acgtgcgcgg cacgcaattt gcagcctttc
     1801 gacgccgcgg cagcgcgccg cgcacgctcg atcactcacg accaaggacg atcggagacc
     1861 catcatggcc aagcaattgg aaggcaaacg tattgcattt cttgcaacgg acggcttcga
     1921 gcagagcgag ctcgagcaac cgtggaatga gatcaagaac gccggcgcga ccgtcgagct
     1981 cttgtccgtg cacaaaggct cgattcaagg catgcgccac atggacaagg gcgatctgtt
     2041 cgaggtcgac gggctcgtgg ccgaagccga cgccgccgat tacgacggcc tcgtgttacc
     2101 cggcggcgtc gcgaatcccg acacgctgcg cacgaatttg cccgccgtcg agttcgtgcg
     2161 cgacttcttc gcccaagcca agccggtcgc cgcgatttgc cacggcccgt ggacgctcgt
     2221 cgaggccgac gcggtgcgcg gccgcacggt gacgtcgtgg ccgagc
//
LOCUS       DASUFX010000203        15611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_170069,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000203 DASUFX010000000
VERSION     DASUFX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15611
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170069"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFV99_07950"
     CDS             complement(<1..618)
                     /locus_tag="VFV99_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059276.1"
                     /translation="MRYVALVAALVAFGACGDDAQTRRDGGGDDDGGGSDTDAMIDAD
                     PNVRGTVTVHIVDKNGSALAGMYVVFIDTDATVTERMTDAGGMAQADVYPNASVTAVR
                     ARSTSYSIATVQALNPGDVITLVSASPTVSSSEDPFSVRVVPMPGADIAASPNGASKS
                     GSIATFTTVAPHGLAVNDRVVVAKVAVAGYNGMWTVASVPSATTFT"
     gene            complement(628..1032)
                     /locus_tag="VFV99_07955"
     CDS             complement(628..1032)
                     /locus_tag="VFV99_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059277.1"
                     /translation="MADEPERQFMGGQKSKMWTYLILMVVLIAIVVVINVVWKNPSAA
                     SSAAKSFFGLPGWALATVVFLVGAVVFWVGLKIETDWPEAFGAFLIAGSVAWFEYIAG
                     WSNFEIGLVVVPYLIPLVVFVLLLMYGMKKSV"
     gene            complement(1077..4232)
                     /locus_tag="VFV99_07960"
     CDS             complement(1077..4232)
                     /locus_tag="VFV99_07960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059278.1"
                     /translation="MVPEDGDPAPNAESSAAFAATIAPSSDAPTPPTKLPDGTQGAPK
                     PPTSDGGAHDQTVAAPSSQQRSALDSGNDPTVAAPIEGSLPPLPEVSASLYQIDKEIA
                     RGGMGKIVAAEDRRLGRSVALKSLLDPSSDQVTRFQREALITARLQHPGIVPVYEAGR
                     WPSGEPFFAMKLVSGRPLDRVISEATKLEDRLAIIPRLAAACDAMAYAHSHRIIHRDL
                     KPGNVLLGDYGETVVIDWGLAKDLDSDHETIERTLQVRHSKPVLDEDQKTQQRTAKRT
                     SEPAGSSTLTVAGAVMGTPAYMAPEQARGEPVDQRADVFSLGAMLYHTLAGVPPYNAR
                     TATDVIAAAALGKIVPLRERERRAPAELVAIVEHAMAPSPFDRYPDAGQLAEELRRFL
                     TGQLVNAHRYTAAQRVMRFIKKHRAAVTIAAIALTTISVGGTIAVRNIVAARDQAQRE
                     KQIAVTRKQAAENLIDYMFSDMKERLDAAGRLDMLAGLGTEVKRYYATLSHMPGGMPR
                     EDEIRMAEAIQLIGEAEHKSGKPDQALATWKGARDRLLEVVGADKTENTRKLRRLIAK
                     LDYEAGVIHQERGKLEEALAHFKNAQAAFEQIKTEDPTSKVLMLETADCHDRLGDLLR
                     LDGKIDEAFEQYMAGKNDREKAASQGNGKVSDEVLALSTSHFKLGSVFQNRGESGSAV
                     DEYHHALRLRETLLEGQPDHVRYQEGVLEVLRELAELERQLGDEKAAVDAYRRAVPLG
                     NALTQRDPTNTEWQYQKGNLLSDLGFALIDSGEFKDGLTQIEASIEVHKDLVARDGKS
                     ARYKIALSRSNTRAGDALIYLGRTGDGVAQYRYALELRQELVDRDAKSVAYRRSIAWS
                     YSKLARAFTLEGDIAHALEAHEQALATRQQLVAEAPSQGGFKNELASSEVELGRLLAT
                     RDGKRAEDLIALGIGRARMLVAGDTINNEWKETLTQGLLAQAELARARGDAKTRGTAL
                     TEALGVAEDAVSKSPQNAHWPGYVAEAHTGLAEVAAAKGDAKAAASEWKRVVETLEPL
                     DKASRLPAPRKPLLDRARARK"
     gene            4253..5008
                     /locus_tag="VFV99_07965"
     CDS             4253..5008
                     /locus_tag="VFV99_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5059279.1"
                     /translation="MSSLHVVRVGQGPRMLMIHGSAADHTTWSIQLASPLRERFTMIA
                     YDRRLTATTVEGHAADAADVLAGERARPALIVGSSFGSVVGLELMRTRPELCAGAVLI
                     EPPMAASDDTTAAPATFLAEFDRIAAAQGGPAAGEFFLRTVLGDEAFDRIPRAFQERS
                     KQKWAEIRADSAALLAYRPRYRELAAVTMQVLLVGGDRSAPYFSETLEALAAALPHAR
                     IETVKGAGHMLHAEARSTFADLLVAFAGQLQIE"
     gene            5021..6448
                     /gene="hflX"
                     /locus_tag="VFV99_07970"
     CDS             5021..6448
                     /gene="hflX"
                     /locus_tag="VFV99_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5059280.1"
                     /translation="MASGGDIFKRATGSERPPAVVVAVQLPGVTDGELSSSIAELERL
                     AKTLGLDPIGRVVQRRAKLAPGVVLGEGKLKELGTWTGGSGEVPVFESVSARRKRVEA
                     EADADADEEGTNEPDEADPEDAVAGEAAKPTRKAEVVIVDHDLTPTQHHNLERATGVD
                     VLDRSSVIIEIFHRHAKTREARLQVELARLRYLAPRLRAASGGGDRVRGGIGGKGAGE
                     SALELDRRRIRDRIAELNHELSQFKGGAQTRRERRSELPTVALVGYTNAGKSSLMRAL
                     TSTDVYVADKLFATLDTTVRRLVPPTDPPILVTDTVGFIKKLPHDLVASFRSTLDEAA
                     DADLLLHVVDASDPAHADQLAVTREVLADIGADSVDSWLLLNKIDRVDEATRAALADH
                     YPNSLQLSAKAPADVAKLRDKLIEHFVGAMQETELDIPWSHQRLVHIVHERTTVLSET
                     HHDDGTKVRVRAPQGVIDNLRDSLK"
     gene            complement(6450..7709)
                     /locus_tag="VFV99_07975"
     CDS             complement(6450..7709)
                     /locus_tag="VFV99_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5059281.1"
                     /translation="MIAVLLLAAGCGRQLNPVFCDEHPNDPDCRNSGLVQIDAPMGEC
                     TQSAMCTGNPNGPVCDTGSQTCVQCIVGIDVSGCAGGTPQCGDDHLCHGCVFDTHCSA
                     SNVCLPNGMCADETAVLYAAPTGTGTVCSQAAPCTFTTAVAAVTATKHIVKLLPSATP
                     YSEPPITIDNTQPMQILADSAIFDPTGTGDGLTVTATNVEIVGLTVKSATGDGVACSG
                     TSILSMRQMNVTDNTGYGINSSGCTITIERSRFSRNVAGGMLLVAGKLEVRNNIIDHG
                     GNAALDKGNVTIQNGNGRFVFNTIANNLSKGGGGRIGGIDCSPTSGLTMQVARNILAD
                     NGAGKPLGGTCTTGTTNYVGKVADIKFSDAANYKLGPMTPTTILRDDPESVADCMTGT
                     KYIDDYEGETRPVNYCDRGADEFHAPP"
     gene            complement(7801..8541)
                     /locus_tag="VFV99_07980"
     CDS             complement(7801..8541)
                     /locus_tag="VFV99_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059282.1"
                     /translation="MRWLGALIALTCAVRVAHAGDVGVVTIGEPSLQPAVVKHFEKWL
                     RKHGHNVIDSPLSVDAINTLANCFTLDDLACARGVFDARAKTPTLVYVGIALNGKNVT
                     FNVYWFDKGKDAVGERRVCEKCEGNAWHGLTDKMLERLAGETTVVYVEKGERHGSRLW
                     PSLVMGAGIATLAAGGIFFYYGSLDGSDQKYIYPDSTPVGIALAAVGAGAAIGGTIWL
                     VQTGGTSRTGPVATATPGGAYVGWVGHF"
     gene            complement(8585..9832)
                     /locus_tag="VFV99_07985"
     CDS             complement(8585..9832)
                     /locus_tag="VFV99_07985"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX5059283.1"
                     /translation="MRTLILITLFAAACSKPNPYYCPGNPDDNCLIDADINAPQGCST
                     SAECTNGAKPICEPMEKVCVACTDGIPGSCGGTTPVCTAAHVCTGCTLHAQCDSTVCL
                     PSGACAETQEVAYVTPNGTDGNGCTKTSPCKTVAAALALGRPTIKILGNIDEAVVVTT
                     GTVQIFGEGGSTLRRTTSQGPVFEARGNGTTVTLSDLIIRDASGATGHGIYVAPGEPV
                     DLTLTHVYAINNAANGLNVQGGKLTLSRSVVSGNSGGGAVVAGLFSITNSLFVANGNG
                     VSTTGGITLTPQTTTTVFKFNTVADNFSSSSSAAVRGVNCTLPQMAANTIVSGNAVSA
                     NCSFGYSLFDTGTTVTGSNIAGDPMFKNIDGLDPLASDYFHIQPASAAVDHADPVSTM
                     STDIDGEARPQGNAPDIGADELH"
     gene            complement(9835..10611)
                     /locus_tag="VFV99_07990"
     CDS             complement(9835..10611)
                     /locus_tag="VFV99_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059284.1"
                     /translation="MRLPILVLASVVSTTAYAERVGVVVTGEATVQPQLAAHLERWLH
                     DHGRDVVPGPLEPDAISTLIDCFVLEDLGCARGVVDTRSKSKAVVFTRAEMTPNGDGT
                     RDIAITGYWFQKDHDALAERRVCEKCNDDRFRATVDELMHALVHEPPPPLEGAALTPE
                     VESTREGGMRKWLPLGLMVAGGTALVAGGIMIAIDEDIDPVGKQHENIRDTATGGVVL
                     GLVGAAALGAGIYLWHHDKHSAPVAAVSNDGGYVGWVGRF"
     gene            complement(10613..12238)
                     /locus_tag="VFV99_07995"
     CDS             complement(10613..12238)
                     /locus_tag="VFV99_07995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059285.1"
                     /translation="MLGTSGKYRLDTRLGGGGMAEVFLGSMLGAEGFSRRVAIKRVLP
                     GFSDNPAFAQMFVAEAQISSQLQHPNIVSVLDFDRDPDGRLFLVMELVDGRDLDALAA
                     SGPLPFPVIIYVISDVLRGLGHAHDLPFGNNMRGLVHRDVSPHNVLLSWEGAVKVSDF
                     GIAKARQASEATASVFIKGKPAYMSPEQANGQSLDGRSDLFAVGVMLWEMLTGRRLFV
                     GEDTRATLAAVLFGQIPRPRAVRPDVPKDLEKVAMKLLERDLPARYATAEQAINDLLE
                     CDDAPKNGREQLIRVLAERFPHNAPVRDSIARSRSGGTPPHMQSQQPYQALGPAQQTI
                     AGGSGSHTPSGATVPPAASIGGYRATPTGTMQPMATVPPRKSGARLAVILVSILVAGM
                     VTFGIILATRKTENPGSVEPPPGGSGTAIATTAGSDSAVPDTAPVAVVNDAALPDAEV
                     VAVAPADAGTETPKKDANVTKPGEKRVGRLVVKAFPVVTVYVDNKKVHDTPVDMNLPV
                     GKHKLRLVNAEVGKDETVSVTIEANKTATIERN"
     gene            complement(12390..13934)
                     /locus_tag="VFV99_08000"
     CDS             complement(12390..13934)
                     /locus_tag="VFV99_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5059286.1"
                     /translation="MQTEASEAARILVVDDERVIREILAEFLTLEGFSVHTVEDGEKA
                     LTELRLHPYDLLITDLKMPRLSGLQLLERIESERLGVLTVLMTGFGTVETAIEAMKKG
                     AYDYLLKPFKVEEVIHVVERALYRQRLQAENIRLREALTIYKVSEAIALTHDIERILD
                     VVLRAALDEVKADVATLHLRDVRTGHYEERVKMVAADGNPQSSLPSPAFGVLTEQFTS
                     GVPILAHGGKASRFFTEQAAPAELASFVAVPLQVRGDMVGVLNVFSFTQGKKFDEGHR
                     KMLAVLASRAASALENARLYGELVHSNEDLRKANLSLEEMFQQTVAGFAQALEESDMY
                     TRGHSERVAVYSEVLARGLTLPDAEIRRIVQAGVMHDVGKIGVRYDMLNKPGKLTPEE
                     IQVFRQHPEKGKRILEPVPCLHGLIDGCWCHHEWYDGGGYPRGLIGQNIPLVGRIVSI
                     ADAYDAMTSDRAYRRALSHEIAIGEIERCAGTQFDPELSNAFVKLIEVWRTQMRERGH
                     SSLIPR"
     gene            complement(13936..14814)
                     /locus_tag="VFV99_08005"
     CDS             complement(13936..14814)
                     /locus_tag="VFV99_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059287.1"
                     /translation="MRGLWQLLVCTLFVIGCGGSSDGPPKPLAKHFDDMYIARIPLDQ
                     KGSVVETQNQWSIAKMENANAEANVQEADGQLHQAQNDQKAAKLAVDSAISAKKSAEQ
                     SADMNRMNNAQKDLRAAEDSEKAAKERVTYFAAYRNYLKKYWRYTQENMYWREAQYES
                     AKATLAKANNIAPKGVNYDDFPKQLTDREKRSQKAKEKSESEKTKAVSARQSWLSAQS
                     AADKATGRTGTLYDPMAPKEGGPTAAGGGISQERPDVVQPLKSNPQPTPPAPAPAPAP
                     APAPAPAPTTAPAPQQ"
     gene            14868..>15611
                     /locus_tag="VFV99_08010"
     CDS             14868..>15611
                     /locus_tag="VFV99_08010"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5059288.1"
                     /translation="MTTLLAVDVGNTNTVLGVFRDGELAAHWRIQTVAQRTSDEYAVL
                     LKTLLDLEGFPWRSIDAGIISSVVPPAVFGLQRFFKRHCGGPALVVGPGIKTGMPILY
                     ENPREVGADRIVNAVAAFEELRAGCIVVDFGTATTWDVVTPKGEYLGGVIAPGIQISS
                     EALYEHAAKLPRVELARPGKVVARNTIASMQAGLVYGYAGMVDALVERIRAEVDYPAR
                     CIATGGLAPLIATETKTIEATDELLTLKGL"
BASE COUNT         2454 a         5166 c         5171 g         2820 t
ORIGIN      
        1 ggtgaacgtc gtcgcggacg gcacgctggc gaccgtccac atgccgttgt agccggccac
       61 ggcgaccttc gcgacgacca cgcgatcgtt gacggcgaga ccgtgtggcg cgaccgtcgt
      121 gaacgtcgcg atcgagcccg acttcgacgc gccgttcgga ctcgccgcga tgtcggcgcc
      181 gggcatcggc acgacgcgaa ccgagaacgg gtcctcggac gacgacacgg ttggcgacgc
      241 gctgaccagc gtgatcacgt cgccgggatt gagcgcctgc accgtggcga tcgagtacga
      301 cgtgctgcgc gcgcggacgg cggtcacgct ggcgttcgga tagacgtccg cctgggccat
      361 accgccggcg tcggtcatcc gctcggtcac cgtcgcgtcg gtatcgatga acacgacgta
      421 catgccggca agcgcactgc cgttcttgtc gacgatgtgc acggtcacgg tgccgcgcac
      481 gttcggatcg gcatcgatca tcgcatcggt gtcgctgccg ccgccgtcgt catcaccacc
      541 gccatcgcgt cgtgtctgcg cgtcatctcc acacgcccca aaggcgacca gcgcggcgac
      601 gagcgcgacg tacctcacgc ggcgatctta gacgctcttt ttcattccat acatgagcaa
      661 cagcacgaac acgacgagtg ggatgaggta gggcacgacg accaggccga tctcgaaatt
      721 cgaccagccc gcgatgtatt cgaaccaggc gaccgagcca gcgatcagaa atgcaccaaa
      781 cgcctcgggc cagtcggtct cgatcttgag cccgacccag aacacgacgg cgccgacgag
      841 aaacacgacc gtggcgagcg cccaaccggg aaggccgaag aagctcttcg cggcgctgct
      901 cgccgccgac gggttcttcc agacgacgtt gatgacgacc acgatcgcaa tcaggacgac
      961 catcaagatg aggtaggtcc acatcttgga cttctgccca cccatgaatt gacgctctgg
     1021 ttcgtccgcc atagcgccca tcgtaacgcg cgccgcgcgg gattgcgcgg taacggctat
     1081 tttcgtgcgc gcgcgcgatc cagcagcggt ttgcgcggcg cgggcagccg gctcgcctta
     1141 tcgagcggct cgagcgtctc gacgacccgc ttccactcgg aggcggccgc cttcgcgtcg
     1201 cccttcgcgg ccgcaacctc ggcgagcccg gtgtgggcct cggcgacgta accgggccag
     1261 tgcgcgtttt gcggcgactt gctgacggcg tcctcggcga caccgagcgc ctcggtcagt
     1321 gcggtaccac gagtcttcgc gtcgccgcgg gcgcgtgcga gctcggcctg tgcgagcagt
     1381 ccttgcgtca gcgtctcttt ccattcgttg ttgatcgtgt cgccggccac gagcatgcgc
     1441 gcgcggccga tgccgagcgc gatcagatct tcggcgcgct tgccatcgcg tgtggcgagc
     1501 agacgaccga gctcgacttc gctcgacgcc agctcgttct tgaagccacc ctgcgatggc
     1561 gcttcggcga cgagctgctg gcgggttgcg agcgcttgtt cgtgtgcttc gagtgcgtgc
     1621 gcgatgtcgc cttcgagcgt gaacgcccgg gcgagcttcg agtacgacca cgcgatcgat
     1681 cgccggtacg cgacactctt ggcatcgcga tcgacgagct cctggcgcaa ttcgagcgcg
     1741 tagcgatact gggcgacgcc atcgccggtg cggccgagat agatcagcgc gtcaccagcg
     1801 cgcgtgttcg agcgcgatag cgcgatcttg taacgggcgc tcttgccgtc gcgcgcgacg
     1861 agatccttgt ggacctcgat cgacgcttcg atctgcgtga ggccgtcctt gaactcaccg
     1921 ctatcgatca gcgcgaagcc caggtcggag agcagattgc ccttctggta ctgccactcg
     1981 gtgttggtgg gatcgcgctg ggtgagcgca ttgccgagcg ggaccgcgcg gcgatacgcg
     2041 tcgactgccg ccttttcgtc gccgagctgg cgctcgagct cggcaagctc gcgcagcact
     2101 tcgagcacgc cttcctgata gcgcacgtgg tccggctggc cttcgagcag cgtctcgcgc
     2161 aggcgcagcg cgtggtgata ctcgtcgacg gcggatccgg actcgccgcg attctggaat
     2221 accgagccga gcttgaagtg gctcgtcgac agcgcgagta cttcgtcgga gaccttgccg
     2281 ttaccctggc tcgccgcctt ctcgcggtcg ttcttgcctg ccatgtactg ctcgaacgcc
     2341 tcgtcgatct ttccgtcgag ccgcagcagg tcgccgagcc ggtcgtggca atccgcggtc
     2401 tccagcatca agaccttcga cgtcgggtcc tcggtcttga tctgctcgaa cgcagcctgc
     2461 gcgttcttga agtgtgcgag cgcttcttcg agcttgccgc gctcctggtg gatcacgccg
     2521 gcctcgtagt cgagcttcgc gatcagccgg cgcagcttgc gcgtgttctc ggtcttgtcg
     2581 gcaccgacga cctcgagcaa gcgatcgcgc gcgcccttcc aggtcgcgag cgcctgatcc
     2641 ggtttgcccg acttgtgctc ggcctcgccg atcagctgga tcgcctcggc catgcggatc
     2701 tcgtcctcgc gcggcatacc gccgggcatg tgcgagagcg tcgcgtagta gcgcttcacc
     2761 tcggtgccga ggccggcgag catgtcgaga cggcccgcag catcgagccg ttctttcatg
     2821 tccgagaaca tgtagtcgat gaggttctcg gcagcttgct tgcgcgtgac cgcgatctgc
     2881 ttctcgcgct gcgcctgatc gcgggcggcg acgatattgc gcaccgcgat cgtgccgcca
     2941 acggagatcg tcgtcagcgc gatggcagcg atcgtgaccg cagcgcggtg cttcttgatg
     3001 aagcgcatca cgcgctgggc ggcggtgtag cggtgcgcgt tgacgagctg accggtgagg
     3061 aagcggcgca gctcctcggc aagctggccg gcatcgggat agcgatcgaa cgggcttggc
     3121 gccatcgcat gctcgacgat cgcgacgagc tcggcaggcg cgcggcgttc gcgttcgcgg
     3181 agcggcacga tcttgccgag cgcggcggcg gcgatgacgt cggtcgcggt gcgcgcgttg
     3241 tatggcggca cgccggcgag ggtgtgatag agcatcgcgc cgagcgagaa gacgtcggca
     3301 cgttgatcga cgggctcacc gcgcgcttgc tcgggcgcca tgtacgcggg cgtgcccatc
     3361 accgcgccgg cgacggtcag tgtcgacgag ccggcgggct ccgacgtgcg cttggcggtg
     3421 cgctgctggg tcttctggtc ctcgtcgagc acgggcttcg aatgccgcac ctgtagcgtg
     3481 cgctcgatcg tctcgtggtc gctgtcgaga tccttcgcga gtccccagtc gatcacgacg
     3541 gtttcgccgt aatcgccgag cagcacgttg cccggcttga gatcgcggtg aatgatgcgg
     3601 tggctgtgcg cgtacgccat cgcgtcgcac gcggcggcga ggcgcggaat gatcgcaagt
     3661 cgatcctcga gcttcgttgc ctcgctgatc acgcgatcga gcggccggcc cgacacgagc
     3721 ttcatcgcga agaacggctc gccactcggc cagcggcctg cttcgtagac cggcacgatg
     3781 ccggggtgtt gcaggcgcgc cgtgatcagc gcctcgcgct gaaagcgcgt gacttgatcg
     3841 ctcgacggat cgagtagcga cttgagagcg accgagcggc cgagccggcg atcctcggcc
     3901 gcgacgatct tgcccatccc gccgcgcgcg atttccttgt cgatctgata gagcgacgcc
     3961 gagacttcgg ggagcggcgg cagcgaaccc tcgatgggcg ccgcgacggt tggatcgttg
     4021 ccgctgtcga gcgcggaccg ctgctggctc gacggcgcag cgaccgtctg gtcgtgagcg
     4081 ccgccgtcac tggtcggcgg tttcggcgca ccctgcgtgc catccggaag cttcgtcggc
     4141 ggcgtcggtg cgtcggagct gggcgcgatg gtcgcggcaa atgccgccga gctctccgcg
     4201 ttaggcgcgg gatctccatc ctctgggacc atcgatcgcc gatagtaccg ccatgtcgtc
     4261 gctccatgtc gttcgtgtcg ggcaaggacc gcggatgctg atgatccacg gcagcgccgc
     4321 cgaccacacg acgtggtcga ttcagctcgc gtcgccgctc cgcgagcgat tcacgatgat
     4381 tgcgtacgat cgccggctca ccgcgaccac cgtcgagggc cacgcggcgg acgcggccga
     4441 cgttcttgca ggtgagcgcg cgcgccccgc cctcattgtc ggctcgagct tcggctcggt
     4501 cgttggcctc gagctcatgc gtacacgacc cgagctgtgt gcaggggctg ttctgatcga
     4561 accgccgatg gcagcgtcgg acgacacgac tgcagcaccg gcgacattcc tcgccgagtt
     4621 cgatcgcatc gctgccgcac agggcggccc ggccgccggc gaattcttct tacggaccgt
     4681 gctcggcgac gaggcgttcg atcggatccc gcgcgcgttc caggaacgct cgaaacaaaa
     4741 gtgggccgag attcgcgccg actcggcggc gctcctcgcg tatcgcccgc gctaccgcga
     4801 gctggcggcg gtgacgatgc aagtactcct cgtcggcggc gaccgctcgg cgccgtattt
     4861 tagcgaaacg ctcgaggcac tggcggcggc gctaccccac gcccgcatcg aaaccgtcaa
     4921 gggtgccggc cacatgctcc atgccgaggc ccggtcgacg ttcgcggacc tgctcgtcgc
     4981 gttcgccggc cagctccaga tcgagtaagg tgcgcgcccc atggcttcag gcggcgacat
     5041 cttcaagcgc gcaacgggca gcgaacgccc gcctgcggtc gtcgtcgccg tgcagctgcc
     5101 aggcgtgacg gacggcgagc tctcgtcgtc gatcgccgag ctcgagcgcc tcgcaaagac
     5161 gctcggtctc gatccgatcg gccgcgtcgt ccagcgccgc gcaaaactcg cacccggcgt
     5221 cgtcctcggc gaaggcaagc tcaaggagct cggcacgtgg accggcggca gcggcgaggt
     5281 gcccgtgttc gagagtgtct ccgcgcggcg taagcgggtc gaagccgagg cggatgcaga
     5341 cgcagacgaa gaggggacca acgaacccga cgaggcggac ccggaagacg ccgtagcggg
     5401 tgaggctgcc aagccgacgc gcaaggccga ggtcgtgatc gtcgatcacg atctcacgcc
     5461 gacccagcat cacaacctcg agcgcgcgac cggcgtcgac gttctcgatc gctcgagcgt
     5521 catcatcgag atcttccatc gccacgcgaa gacccgcgaa gcccgcctgc aggtcgagct
     5581 cgcgcggttg cgctacctgg cgccgcgcct gcgcgccgct tcgggtggtg gcgatcgcgt
     5641 gcgcggtggc atcggtggca agggcgccgg cgagagcgca ctcgagctcg atcgccgccg
     5701 cattcgcgat cggatcgccg agctcaacca cgagctctcg cagttcaaag gcggtgcaca
     5761 gacccggcgc gaacgccgca gcgagctacc cacggtcgcg ctcgtcgggt acacgaacgc
     5821 cgggaagtcg tcactgatgc gagcgctgac cagcaccgac gtttatgtcg cggacaagct
     5881 gttcgcgacg ctcgacacga cggtgcgccg gctcgtcccg ccgaccgatc cgccgatcct
     5941 cgtcactgat acggtcggct tcatcaagaa gctgccgcac gatctcgtcg cgtcgttccg
     6001 ctcgacgctc gacgaggcgg ccgacgcaga tctgttgttg cacgtcgtcg acgcgagcga
     6061 tcccgcgcac gccgatcagc tcgccgtcac gcgcgaggtg ctcgccgaca tcggcgcgga
     6121 cagcgtcgac tcgtggctac tgctcaacaa gatcgatcgc gtcgacgagg ccacccgtgc
     6181 tgcgctcgcg gatcactatc caaactcgct gcagctctcg gccaaggcac ccgcagatgt
     6241 cgcgaagctg cgtgacaagc tgatcgagca tttcgtcggc gcgatgcagg aaacggagct
     6301 cgacattccg tggtcgcacc agcggctcgt tcacatcgtg cacgagcgca cgacggtgct
     6361 ctccgaaacg caccacgacg acggcacgaa ggtccgcgtg cgcgcaccgc agggcgtgat
     6421 cgacaacctg cgtgactcgc tcaagtgact cagggcggcg cgtggaactc gtcggcgccg
     6481 cggtcgcagt agttgacggg ccgcgtttcg ccttcgtagt cgtcgatgta cttcgtaccc
     6541 gtcatgcaat cggcgaccga ctcgggatcg tcgcgcagga tcgtcgtcgg cgtcatcggg
     6601 ccgagcttgt aattggcggc atccgagaac ttgatgtcgg cgactttgcc gacgtagttc
     6661 gtcgtgccgg tcgtgcacgt gccgccgagc ggcttgccgg cgccattgtc agcgagaata
     6721 ttgcgggcga cctgcatcgt gaggccgctg gtcggcgagc agtcgatgcc gccgatgcgt
     6781 ccgccgccgc ccttggacag gttgttcgcg atcgtgttga acacgaagcg cccgttcccg
     6841 ttctggatcg tcacgttgcc cttgtcgagg gccgcgttgc cgccgtggtc gatgatgttg
     6901 ttgcgaacct cgagcttgcc cgcgacgagc aacatgccac ccgccacgtt gcgggaaaat
     6961 cggctgcgct cgatcgtaat cgtgcagccg ctcgagttga tgccgtaacc ggtgttgtcg
     7021 gtgacgttca tctggcgcat cgacaagatc gacgtgccgg agcacgcgac accgtcgccg
     7081 gtcgcgctct tgacggtgag gccgacgatc tcgacgttcg ttgcggtcac cgtgagcccg
     7141 tcgccagtcc ctgtcggatc gaaaatcgcg ctgtcagcca ggatctgcat cggctgcgtg
     7201 ttgtcgatcg tgatcggcgg ctcgctgtag ggcgtcgcgc tcggcagtag cttgacgatg
     7261 tgcttcgtcg cggtgaccgc ggcgacagcc gtcgtgaacg tgcacggtgc cgcttgcgag
     7321 cacacggtac cggtgccggt tggcgccgcg tacagcacgg cggtctcgtc ggcacacatg
     7381 ccgttcggca agcacacgtt cgaggcgctg caatgcgtgt cgaagacgca gccatggcag
     7441 aggtggtcgt caccgcattg cggtgtgccg ccggcgcagc cgctgacgtc gatgccgacg
     7501 atgcactgca cacacgtctg cgagccggtg tcgcagaccg ggccgttcgg gttgccagtg
     7561 cacatcgcgc tctgcgtgca ctcgcccatc ggcgcatcga tctgcacgag gccggagtta
     7621 cggcagtccg gatcattcgg gtgctcgtcg cagaacaccg gattgagttg cctgccacag
     7681 cctgcggcga gcaacaacac ggcgatcacg gctcctacga gttgccgggc catcctcatc
     7741 ggtaggttcg cgcggaacgt cagcattcga aagcccattg ttctcgctcg cgctggcagg
     7801 tcagaagtga ccgacccaac cgacataggc gccaccaggt gtggcagttg ccacaggacc
     7861 ggtcctcgag gtgccacctg tctggacgag ccagatcgtg ccgccgatcg cggctcctgc
     7921 gccgacggct gctagcgcga tgccgacagg cgtcgagtct gggtagatgt acttctggtc
     7981 gctaccgtcg agggagccgt aatagaagaa gatgccgccc gccgcgagcg tcgcgatccc
     8041 ggcgcccatc accaggctcg gccacagccg agagccgtgg cgctcgccct tctccacgta
     8101 gacgaccgtg gtttcacccg cgagccgctc gagcatcttg tcggtgaggc catgccacgc
     8161 gttgccttcg cacttctcgc acacgcgtcg ctcgccgacc gcgtccttgc ctttgtcgaa
     8221 ccagtacacg ttgaacgtga cgttcttgcc gttcagcgcg atgccgacat agacgagcgt
     8281 cggcgtcttc gcgcgcgcgt cgaacacgcc acgcgcacac gcgagatcgt cgagcgtgaa
     8341 gcagttcgcg agcgtgttga tcgcgtcgac cgacagcgga ctgtcgatca cgttgtggcc
     8401 gtgcttgcgc agccacttct cgaagtgctt cacgacagcc ggctggagcg acggctcacc
     8461 gatcgtgacc acgccgacgt cacccgcatg ggcgacgcgc accgcacacg tcagcgcgat
     8521 cagggccccg agccaacgca tccgaaatca atcgtatcgc aaccggcgcg agcgtgcctg
     8581 attgctagtg gagctcgtcg gcgccgatgt caggcgcatt accctgcggg cgcgcctctc
     8641 cgtcgatgtc ggtcgacatc gtgctgacgg ggtccgcgtg atcgacagcc gcgctcgcgg
     8701 gctggatatg gaagtagtcg ctggcgagcg gatcgaggcc gtcgatgttc ttgaacatcg
     8761 gatccccggc gatgttcgaa ccggtgacag tcgtgccggt atcgaatagc gaatagccaa
     8821 acgagcagtt tgccgacacg gcgttgccgg acacgatcgt gttcgcggcc atctgcggca
     8881 gcgtgcaatt cacgccacgc accgccgcgc tgctggagct cgagaagttg tccgccacgg
     8941 tgttgaactt gaacaccgtg gtcgtcgtct gcggcgtgag tgtgatgccg ccagtcgtcg
     9001 agacgccgtt gccgttcgcg acgaacagcg agttcgtgat gctgaacaac ccagcgacga
     9061 ccgcgccgcc gccgctgttt cccgacacga ccgagcgcga cagcgtcagc ttgccgcctt
     9121 ggacgttgag cccgttcgcg gcgttgttga tcgcgtagac gtgagtgagc gtgaggtcga
     9181 cgggctcgcc gggcgcgacg tagatcccat ggccggtcgc gccggaggcg tcgcgaatga
     9241 tgaggtcgct gagcgtcaca gtcgtgccgt tgccacgcgc ctcgaacacg gggccttggc
     9301 tcgtcgtgcg gcgcaacgtg gagccgcctt cgccgaaaat ctgaaccgtg ccggttgtga
     9361 cgacgaccgc ctcgtcgatg ttgccgagga tcttgatcgt gggtcggccg agcgcgagcg
     9421 cggcagcgac cgtcttgcac ggcgaggtct tcgtgcagcc gttgccgtcc gtaccgttcg
     9481 gcgtcacgta cgcgacctct tgcgtctccg cgcatgcgcc gctcggcaga cacaccgtcg
     9541 aatcgcattg cgcgtgcagc gtgcagccgg tgcagacgtg cgcggccgtg cacacgggcg
     9601 tcgtgccgcc gcacgaaccg ggtatgccgt cggtgcatgc aacgcagact ttctccatcg
     9661 gctcgcagat cggcttcgcg ccgttcgtgc attcggcaga cgtcgagcaa ccctggggcg
     9721 cgttgatgtc ggcgtcgatc aggcagttgt cgtcgggatt gccggggcag tagtacgggt
     9781 tcggcttgct gcacgcggcg gcaaatagcg tgatcaggat cagcgtacgc atctctagaa
     9841 cctcccgacc caaccgacgt agccgccgtc attcgaaact gccgcaacgg gcgcggagtg
     9901 cttgtcgtga tgccacagat agatgccagc gccgagtgct gccgccccga cgagcccgag
     9961 caccacaccg cccgtcgcgg tgtcgcggat gttctcgtgc tgcttgccga ccggatcgat
    10021 gtcctcgtcg atcgcgatca tgatcccgcc cgcaaccaat gcggtgccac cggcgaccat
    10081 gaggccgagc ggtagccatt tccgcatgcc gccttcgcgc gtcgattcga cctcgggcgt
    10141 tagcgcagcg ccttcgagcg gcggcggcgg ttcgtgcacg agtgcgtgca tcagctcgtc
    10201 gaccgtggcg cggaaccggt cgtcgttgca cttctcgcac acgcgacgct ccgcaagcgc
    10261 atcgtggtcc ttctggaacc agtagccggt gatcgcgatg tcacgcgtgc cgtcgccgtt
    10321 cggcgtcatc tcggcgcgcg tgaacacgac tgccttcgac ttcgagcgcg tgtcgacgac
    10381 gccgcgcgca caaccgagat cctcgagcac gaaacagtcg atcaacgtgc tgatcgcgtc
    10441 gggctcgagt gggccgggaa caacgtcgcg gccgtggtcg tggagccagc gttcgaggtg
    10501 cgccgcgagc tgcggctgca cggtcgcttc gccggtgacg acgacgccga cgcgctccgc
    10561 gtaggcggtg gtggagacaa cactcgcgag cacgagaatc ggcagtcgca tgtcagttcc
    10621 gctcgatggt tgccgtcttg ttcgcctcga tggtcacgga aaccgtctcg tccttgccga
    10681 cttcggcgtt gacgaggcgg agcttgtgtt tgccaacggg gagattcatg tcgacgggcg
    10741 tgtcgtgaac tttcttgttg tcgacgtaca cggtgacgac cgggaacgcc ttcacgacga
    10801 gccgtcccac ccgtttctcg cctggtttgg tgacgttggc atcttttttg ggtgtctctg
    10861 tgccggcatc cgctggcgcc accgcgacga cttctgcgtc ggggagtgca gcgtcgttca
    10921 caaccgcgac gggcgccgta tccggcactg ccgaatcgga tccagcggtg gtcgcgatcg
    10981 cggttccgct gcctcctggt ggcggctcga ctgaacccgg attctcggtt tttcgtgtcg
    11041 ccaggatgat gccgaacgtc accatgcccg cgacgaggat cgagaccaga atgacagcga
    11101 gccgcgcacc gctcttgcga ggcggcacgg tcgccatcgg ctgcatggtg ccggtcggcg
    11161 tcgcgcgata gccgccgatg ctcgcggcgg gcgggacggt tgctccgctc ggcgtgtggc
    11221 tcccgctgcc gcctgcgatc gtttgctgcg ccggaccgag cgcttggtac ggctgctgcg
    11281 attgcatgtg gggcggtgtg ccgccgctgc gcgagcgcgc gatgctgtcg cggacgggcg
    11341 cgttgtgcgg aaaccgctcc gcgagcacgc ggatcaattg ctcgcgcccg ttcttcggcg
    11401 cgtcatcgca ctcgaggaga tcgttgatcg cttgctcggc ggtcgcgtag cgcgctggca
    11461 aatcgcgctc gagcaatttc atcgcgacct tctcgagatc cttcggcaca tcgggccgca
    11521 ccgcgcgcgg gcgcggaatc tgaccgaaca acaccgcggc gagcgtcgcg cgggtgtctt
    11581 cgccgacgaa caggcgccgg ccggtgagca tctcccacag catcacgccg accgcgaaca
    11641 ggtcgctgcg tccgtcgagc gattggccgt tcgcctgttc cgggctcatg tacgccggct
    11701 tgcccttgat gaacaccgac gcggtggcct cgctcgcttg ccgcgctttc gcgatgccga
    11761 agtcgctgac cttgaccgcg ccctcccacg acagcagcac gttgtgcggc gacacgtcgc
    11821 gatgcacgag gccgcgcatg ttgttgccga agggcaggtc gtgcgcgtgg ccgagaccgc
    11881 gcagcacgtc gctgatcacg tagatgatga ccggaaacgg cagcggtcca ctcgctgcga
    11941 gcgcgtcgag gtctctgcca tcgacgagct ccatgacgag gaacagccga ccgtcgggat
    12001 cgcgatcgaa gtcgagcacc gacacgatgt tcgggtgctg cagctgcgag ctgatctgcg
    12061 cctcggcgac gaacatctgc gcgaacgccg ggttgtcgga gaacccgggc agcacgcgct
    12121 tgatcgcgac gcgccgcgag aagccttcag cgccgagcat cgagccgagg aacacctcgg
    12181 ccatgccacc gccgcctagt ctggtatcga ggcgatattt cccggacgta ccgagcacgt
    12241 gcgtcggcag tatagatgat cagcgcgcgc gaacgcatgc gggcgatcga tcccgcgcat
    12301 gcgaagtcct cgtcgagacc acctggcccg aagatcaggc cgcgctcgga agtctcgtgg
    12361 tccgcagcga attgcggcag cgtcgaccat cagcgtggaa tcagcgagct atggccgcgc
    12421 tctcgcatct gcgtgcgcca gacctcgatc agcttcacga acgcattcga cagctcggga
    12481 tcgaactggg tgcctgcaca gcgctcgatc tcgccgattg cgatctcgtg gctcagcgcc
    12541 cgacggtagg cacggtccga cgtcatcgcg tcgtaggcgt cggcgatcga tacgatgcga
    12601 ccgacgagcg gaatgttctg gccgatcaag ccgcgcggat agccgccgcc gtcgtaccac
    12661 tcgtggtggc accagcagcc gtcgatcagc ccgtggagac acggcaccgg ctcgaggatg
    12721 cgcttgccct tctccgggtg ctgacggaac acctggatct cttccggcgt cagcttgccg
    12781 ggcttgttca gcatgtcgta acgcacgccg atcttgccga cgtcgtgcat gacgcccgcc
    12841 tgcacgatgc ggcgaatctc ggcatcgggc agcgtgaggc cgcgcgcgag cacctcgctg
    12901 tacacggcga cacgctcgga gtggccgcgc gtgtacatgt cgctctcttc gagcgcttgc
    12961 gcgaagccgg cgaccgtctg ctggaacatc tcttcgagcg agaggttcgc tttgcgcaga
    13021 tcttcattgc tgtggacgag ctcgccgtag agccgcgcat tctcgagcgc actcgctgca
    13081 cgcgacgcga gcacggcgag catcttgcga tgaccttcgt cgaacttctt gccctgcgtg
    13141 aacgagaaca cgttgagcac gccgaccatg tcgccgcgca cctgcagcgg caccgcgacg
    13201 aacgacgcga gctcagctgg cgccgcttgc tcggtgaaga agcgcgaggc cttgccgccg
    13261 tgcgcgagga tcggcacacc gctcgtgaac tgctcggtga gcacgccgaa tgcgggtgac
    13321 ggaagggagc tctgcggatt tccgtccgcc gcgaccatct tcacgcgctc ttcgtagtga
    13381 ccggtgcgca cgtcccgcag atgcagcgtc gcgacgtcgg ccttcacctc gtccagcgcg
    13441 gcgcgcagca ccacgtcgag gatgcgttcg atgtcgtggg tcagcgcgat cgcttccgag
    13501 accttgtaga tcgtgagcgc ttcgcgcagt cggatgttct ccgcctgcaa gcgctggcga
    13561 tagagcgcgc gctcgacgac gtggatgacc tcctcgacct tgaacggctt gagaaggtag
    13621 tcgtacgccc ccttcttcat cgcctcgatc gcggtctcga ccgtgccgaa gccggtcatc
    13681 agcacggtca gcacaccgag ccgttcggac tcgattcgct cgagtaactg aagccccgac
    13741 agccgaggca tcttgaggtc cgtgatcagt agatcgtacg gatgcagacg gagctcggtc
    13801 agcgccttct caccgtcttc gacggtgtgg accgagaagc cctcgagcgt gaggaactcg
    13861 gcgaggattt cgcggatcac tcgctcgtca tcgacgacaa ggatccgcgc cgcttccgat
    13921 gcttctgttt gcactctact gttgcggagc gggagcagta gtcggtgccg gagccggagc
    13981 cggagcagga gcaggagcag gagccggcgc gggcggcgtc ggctgcgggt ttgacttcag
    14041 cggctgaacg acatcgggcc gctcttggct gatgccaccg ccagcggccg tcgggccacc
    14101 ttccttcggt gccatcgggt cgtacagcgt gccggtgcgg cccgtcgcct tgtcggcagc
    14161 cgactgcgcg gagagccagc tctggcgcgc cgagaccgcc ttggtcttct cgctctcgga
    14221 cttctccttc gccttctgcg agcgcttctc gcgatccgtg agctgcttcg ggaagtcgtc
    14281 gtagttcacg cccttcggcg cgatgttgtt cgccttcgcg agtgttgcct tcgcgctctc
    14341 gtactgggct tcgcgccagt acatgttctc ctgcgtgtag cgccagtact tcttgaggta
    14401 attgcgatac gcagcgaagt acgtgacgcg ctccttcgcg gctttctcgg agtcttccgc
    14461 ggcgcgcagg tctttctgcg cgttgttcat ccggttcatg tcggccgatt gctcggcgct
    14521 cttctttgcg gagatcgcgg agtcgaccgc gagcttggcc gccttctgat cgttctgggc
    14581 ctggtgcagc tggccatcgg cctcttgcac gttcgcttcg gcgttcgcgt tctccatctt
    14641 cgcgatcgac cattggttct gcgtctcgac gacgcttccc ttttgatcga gcggaatacg
    14701 cgcgatgtac atgtcatcga agtgcttcgc gagtggcttc ggaggaccgt ccgaagagcc
    14761 gccgcaaccg atgacaaaaa gcgtacagac gagaagctgc caaagtcccc gcataggtga
    14821 gcccgagcct actacggccc gccgcggcga gctaccatct cggaggaatg acgacactac
    14881 tcgccgtcga cgtcggaaac accaacacgg tgctcggggt gtttcgcgat ggcgagctcg
    14941 ccgcccactg gcgcatccag accgtggctc agcgcacctc cgatgaatac gcggtgctgc
    15001 tgaagacgct gctcgatctc gaaggctttc cgtggcggag catcgatgcc ggcatcatct
    15061 cgtcggtggt tccaccggcc gtctttggcc tgcagcggtt cttcaaacgc cactgcggcg
    15121 ggcctgcgct cgtggtcgga cccggcatca agaccgggat gccgatcctg tacgagaacc
    15181 cgcgcgaagt cggtgcggac cgcatcgtca acgcagttgc tgcgttcgag gagctgcgcg
    15241 ccggctgcat cgtggtcgac ttcggcaccg ccacgacctg ggatgtggtg acgcccaaag
    15301 gtgaatatct cggcggcgtg atcgcgccgg gcatccagat cagctcggag gcgttgtacg
    15361 agcacgccgc caagctgccg cgcgtcgagc tggcacgacc cggcaaagtc gtcgcgcgaa
    15421 acacgatcgc ctcgatgcag gccggtctcg tttacggcta cgcgggcatg gtcgacgcgc
    15481 tcgtcgaacg cattcgcgcc gaggtcgatt accccgcgcg ctgcatcgcg accggcgggc
    15541 tcgcgccgct catcgcaacc gaaacgaaga cgatcgaggc gaccgacgag ctgctcacgc
    15601 tcaaggggct c
//
LOCUS       DASUFX010000204         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_866030,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000204 DASUFX010000000
VERSION     DASUFX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866030"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFV99_08015"
     CDS             complement(<1..1162)
                     /locus_tag="VFV99_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5059289.1"
                     /translation="MGSETKASGPDLRAGVDASSVGATLLGHVDDQPVLLVRHGQAIR
                     AVAATCTHYGGPLAEGIVTDGTIHCPWHHACFDLATGRPGGPAIAPLACWNVIVDGNR
                     VRVGEPLVDKPAEVRAPDRVVIVGGGAAGVACAEALRTQGHRGSITIVTGEGSDPVDR
                     PNLSKDYLAGSAPEEWVYLRTADMLREHDIALVAQAATRIDTKQRVVVTGAGEHGFDA
                     LLIATGCEPIRLPLDGASLPHVHTLRTLADSKAIASAATAGTNAVVIGASFIGLEVAA
                     SLRARGAAVTVVGPETTPLARVLGDQVGAFIQRVHESKGVVFKLGRKPASITAEHVVL
                     DDGTKLPASLVVMGVGVKPRTQLAEAAGLPIDRGIVVDAQLRVAPGIWAAGDV"
     gene            1249..2406
                     /locus_tag="VFV99_08020"
     CDS             1249..2406
                     /locus_tag="VFV99_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5059290.1"
                     /translation="MSQARRAPKGTYELVLLPGDGIGAEVIADARALLDVVGPAIGVG
                     FALEEIPCGGKFYLEHGSRDWPEGAEEKCAAADAILLGAVGWNGPDGAPVTMADGKMA
                     GWSPVIGNRMKLDLYANVRPVRCLPGTKHNISGQHTSVWKPEAVDMVILRENTEGLYS
                     GIGERTRERATDLRVITQRASERVIRKAFEISHDRGKGAPSDGKKRVTCIVKHNVLQG
                     CRLFLDVFRAVGKDFPDIEQDVAIVDAFAMFVLTQPERYDVCVTTNMFGDILTDLASV
                     LQGGMGMAVGCNVGDDHAMFEPIHGSAPPLAGKDRANPMAMLLATAEALGWLGKRYAD
                     DRLVRAKKAIDESVATIIARGEPLTTDLGGTSTRSAVATAIRDDVARRLSH"
     gene            2517..2600
                     /locus_tag="VFV99_08025"
     tRNA            2517..2600
                     /locus_tag="VFV99_08025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2550..2552,aa:Leu,seq:caa)
     gene            complement(2663..2848)
                     /locus_tag="VFV99_08030"
     CDS             complement(2663..2848)
                     /locus_tag="VFV99_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059291.1"
                     /translation="MKKIAIVLFVVAAALAGCGKKKAPAAPAGGEAGSAAPAEAGPGS
                     AEAGSAAPAEGGGSGGM"
     gene            complement(2912..3112)
                     /locus_tag="VFV99_08035"
     CDS             complement(2912..3112)
                     /locus_tag="VFV99_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059292.1"
                     /translation="MKIIISVLLAASLMACGGKAKKTTTPDNKTGTTEMKPDGTGGTT
                     YGGAQTTPPATPASGTDPCAGQ"
     gene            complement(3125..3364)
                     /locus_tag="VFV99_08040"
     CDS             complement(3125..3364)
                     /locus_tag="VFV99_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059293.1"
                     /translation="MELKGQGQLADAWAAFEELIAKFPDYVAAYLQAGGVLVGLGRKD
                     EAAEIYGKGIEVAQRRGDQHARREIEAALAEITPV"
     gene            3581..>3817
                     /locus_tag="VFV99_08045"
     CDS             3581..>3817
                     /locus_tag="VFV99_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059294.1"
                     /translation="MQRAFSRFLGVDLGGGRGKTTAVAELRIGTTGSAEVIEVATRSG
                     RLPWTDDTLIGRLAIPDPERVIAVDAPLTTTACGR"
BASE COUNT          664 a         1281 c         1259 g          613 t
ORIGIN      
        1 cgacatcgcc tgcggcccag attcccggtg cgacgcgcag ctgcgcatcg acgacgatgc
       61 cgcgatcgat cggcaaccca gcagcctcgg cgagctgcgt gcgcggcttg acaccgacgc
      121 ccatcacgac cagcgacgcg ggcagcttcg tgccgtcgtc gagcacgacg tgctcggcgg
      181 tgatcgacgc cggcttgcgg ccgagcttga acacgacgcc tttgctctcg tgaacgcgct
      241 ggatgaacgc accgacctga tcaccgagca cgcgcgcgag tggcgtggtc tcgggaccga
      301 cgacggtgac cgcagcgccg cgcgcacgca gcgacgccgc gacctcgaga ccgatgaagc
      361 tcgcaccgat gacgacggcg ttggtaccgg ccgttgccgc gctcgcgatc gccttgctgt
      421 cggcgagcgt acgcagcgtg tggacatgcg gaagcgacgc gccgtcgagc ggcagccgga
      481 tcggctcgca gccggtcgcg atcagcagcg cgtcaaaccc gtgctcgccg gcgccggtga
      541 ccacaacccg ctgcttggtg tcgatgcgcg ttgcggcctg cgcgacgagc gcgatgtcgt
      601 gctcgcggag catgtccgcc gtccgcaagt agacccactc ttccggcgca ctgccggcga
      661 gatagtcctt cgacaaattc ggccggtcga ccggatcaga gccttcgccg gtaacgatcg
      721 tgatcgaacc gcggtggccc tgggtgcgca gcgcttcggc acacgcgaca cccgcggcgc
      781 caccaccgac gatcacgacg cgatcgggtg cacgtacctc cgcgggcttg tcgacgagcg
      841 gctcgccgac gcgcacgcga ttgccgtcga cgatcacgtt ccaacacgcg agcggtgcga
      901 tcgccggacc gccgggacgt ccggttgcta gatcgaagca cgcgtgatgc catgggcagt
      961 gaatcgtgcc gtcggtgacg atgccctccg cgagcgggcc gccgtagtga gtacacgtcg
     1021 cggcgacggc gcgaatcgcc tgaccatgcc gcacgagcaa caccggctga tcatcgacgt
     1081 ggccgaggag cgtggcgccg acgctgctcg cgtcgacccc ggcgcgcaag tccggaccac
     1141 tcgctttcgt ttcgcttccc atgtgtccct cctgatcaga gtgctcgtcg cttccaaacg
     1201 tgacaggcga tcgcgccgcg accatagcgc ggtgctaccc tgcgcgcgat gtcacaagcc
     1261 cgccgcgcac ccaaaggaac gtacgagctc gtgctgctcc cgggcgatgg catcggcgcc
     1321 gaagtgatcg ccgatgcgcg tgcattgctc gatgtcgtcg gtcccgcgat cggcgtcggc
     1381 ttcgcgctgg aggagattcc gtgcggcggc aagttctacc tcgagcacgg gagtcgcgac
     1441 tggccggagg gcgccgagga gaagtgcgcc gccgccgatg cgatcctgct cggtgccgtc
     1501 ggttggaacg gtcccgacgg cgcgccggtc acgatggccg acggcaagat ggcgggctgg
     1561 tcgccggtga tcggcaaccg catgaagctc gatctctacg cgaatgtgcg gcccgttcgc
     1621 tgcctgccgg gcacgaagca caacatctcg ggtcagcaca cgtcggtgtg gaaacccgaa
     1681 gcggtcgaca tggtgatcct gcgcgagaac accgaagggc tctactcggg catcggcgag
     1741 cgcacccgag agcgcgcgac cgacctgcgc gtgatcacgc agcgcgcgtc ggagcgcgtg
     1801 atccgcaagg cgttcgaaat ctcccacgac cgcggcaagg gcgcgccgag cgacggcaag
     1861 aagcgtgtga cctgcatcgt gaagcacaac gtgctgcagg gctgccggtt gttcctcgac
     1921 gtgtttcgcg cggtcggcaa agacttccct gacatcgaac aagacgtcgc gatcgtcgac
     1981 gcgttcgcga tgttcgtgct cacgcagccc gagcgttacg acgtctgcgt gacgacgaac
     2041 atgttcggcg acatcctcac cgacctcgca tcggtgctcc agggcggcat ggggatggcg
     2101 gtcggctgca acgtcggtga cgaccacgcg atgttcgagc cgatccacgg ctcggcgccg
     2161 ccgctcgccg gcaaggaccg cgcgaatccg atggcgatgt tgctcgcgac ggccgaagcc
     2221 ctcggctggc tcggcaagcg ctacgcggac gaccgcctgg tccgcgccaa gaaagcgatc
     2281 gatgaatcgg tcgcgacgat catcgcgcgc ggcgagccgc tcaccacgga tctcggcggc
     2341 acatcgacac gcagcgcggt tgcgacagcg atccgcgacg atgtcgcgcg tcggctatcg
     2401 cactgaccgt tttgtgctag aaaagcgcgc cgcggtgctc gagggccgcg gctgagccgg
     2461 gatggagaaa tggtagtact cggggggctt caaagccctt ccccttgaaa cgaagagccg
     2521 ggatggagaa atggtagact cgggggtctc aaaagcccct tccccttaaa ggagtgtcgg
     2581 ttcgagtccg actcccggca ccgaaaaacg aaacgcgccg atcaaacgat cgacgcgttc
     2641 cgaaagtagc ctcgaaaacg gcttacatgc cgcccgagcc accaccctca gccggggccg
     2701 ccgagccagc ctctgccgag cccgggcccg cttcggccgg agccgccgag cccgcctcgc
     2761 cgccggccgg agccgcggga gccttcttct tgccacagcc cgcaagcgcc gcggcgacca
     2821 caaacagaac aatcgcaatc ttcttcatac tctctagtcc tctgttggtt tgttctcgct
     2881 gctggcgctt gccagtcagc aaaaaaggaa actactggcc cgcgcaggga tccgtgccgc
     2941 tcgcaggcgt cgccggcgga gtcgtctgcg cgccgccgta ggtggtgccg cccgtaccgt
     3001 ccggcttcat ctcggtcgtg ccggtcttgt tgtcgggcgt cgtcgtcttc ttggccttgc
     3061 cgccacatgc catcagcgaa gcagcgagca gcaccgaaat gatgatcttc atcgtcagat
     3121 ctccttaaac cggggtgatt tccgcgagag ccgcctcgat ttcgcggcga gcatgctgat
     3181 cgccgcggcg ctgggcaact tcgatgccct tgccatagat ctccgcggct tcgtccttgc
     3241 ggccgagccc gaccaggacg ccgccggcct gcaaataggc cgcgacgtag tctggaaatt
     3301 tcgcaatcag ctcctcgaag gccgcccacg cgtccgccag ctgaccctgc cccttgagct
     3361 ccatcgcaag gccataccga gggaacgggt ccgtcggagt cttcgcgatg aagctacgaa
     3421 aagaggcgat gcgatccata agctacgggc cgacgtttaa cgtggaaggt cgtgcggcct
     3481 tacaggggtt gcgacgatat ctcaggactt ggggccaaat gccagtgccc aaacaacatt
     3541 tctggcatgg tgatcgtgct acaaagggta aagagcgcgg gtgcagcgag cgttttccag
     3601 gttccttggc gtcgatctcg ggggtggccg agggaagacc actgccgttg cagagctccg
     3661 gattggcacc accggtagcg ccgaggtcat cgaggtcgcg acccgcagcg ggcggctgcc
     3721 gtggaccgat gacaccctga tcggccggct cgcgatccca gatcccgagc gcgtgatcgc
     3781 tgtcgatgcg ccgctgacga cgacggcgtg cggacgc
//
LOCUS       DASUFX010000205         7780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1977154, whole genome shotgun sequence.
ACCESSION   DASUFX010000205 DASUFX010000000
VERSION     DASUFX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977154"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..793
                     /locus_tag="VFV99_08050"
     CDS             32..793
                     /locus_tag="VFV99_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX5059295.1"
                     /translation="MIGELVMGVYDGLRGAAYLATHPRLWKWVLAPAIVAALLLFVVI
                     GTTLSALSTPIAAIAAFLPGSWADNLLELAAGIVLTIASFSIFISVAALIAGPFNEEL
                     SESVEEHVTGVPGPKFSIGRFLVDLLIGIIHAARRVFVYLVVMGVLLIVGVVVPGIGT
                     VIAAVLGFIATARFASYDAFDAIWSRRRYRYRQKTGYLREHRWRTLGLGAVCAVPLVV
                     PGLNVIALAIGATAATLRVVEQERLRAAVGAATKS"
     gene            complement(780..2954)
                     /locus_tag="VFV99_08055"
     CDS             complement(780..2954)
                     /locus_tag="VFV99_08055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX5059296.1"
                     /translation="MRKRLLAAILFAGCGSSPQPKTTTAAPTPPTTTTDPTTAPAPTP
                     ATTTAKGGELVVPTPGNPPAPQKGGAPEIGTWGFDLKGMNTKLAPGASFYNYSNGTWL
                     ETTPIPEDKSNYGTFGILSDKSDERTKEIIVNAKGKPGSEAKKIADYYASFMDEAGIE
                     KKGIAPIKGELAKIKAVKSAKELVPLFGYFARHFRTSPFPTIVGQDDKAPETHIANIG
                     QGGLGLPDRDMYDAKNAQFEPLREGYKKYIAAMLTLAGEKDAEKRAADIYALEEKIAA
                     THWTRIENRDPQKTYNKLTIDELQKTAPDIDWKAWLKATGLEKVKEVNVNQPSAIAGT
                     AKLVASEPIGVWKDYLTVIMLGEAAPYLPKAFVDTHFEMYGKTLSGTPQIKDRWKRGV
                     DQITGTMGEAVGKLYVEKYFTPATKAQADQLVKNLLTAMGQRLDALAWMGPETKTKAK
                     AKLATYNPKIGYPKKWRDYSKLTIKAGDAFGNADRASAFEYDRRLAKLGKPVDRDEWG
                     MTPMTVNAYYNPALNEIVFPAAILQPPFFDPNADDGVNYGGIGAVIGHEISHGFDDQG
                     SQYDATGKLENWWTKDDETKFKAATAQLVAQYNTYCPFPAAGGKPAQCVKGELTLGEN
                     IADLAGLTIAYQAYKLSLNGKDAPVIDGLSGDQRFFLGWAQAWRRLYRDQELMQRLVT
                     DPHSPSEFRTSVVRNLDAWYDAFKIQPGDKLYLAPNKRVRIW"
     gene            complement(3037..3543)
                     /locus_tag="VFV99_08060"
     CDS             complement(3037..3543)
                     /locus_tag="VFV99_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059297.1"
                     /translation="MAKRWAIGVDVGPESYKPDADNAQRTEFGQFELAARYRIRAPIE
                     LGLALHLAGSKDISAGGLYVDFRYRFLAEQPLNLYGLAGLGVLAVAHKDASDDARQGR
                     GSLRLGIGGEYRWNWFALILEVRLVAVGENKDLGALPPGTPEPVDYQLSRYSLSGASF
                     ALGANFYF"
     gene            complement(3627..3833)
                     /locus_tag="VFV99_08065"
     CDS             complement(3627..3833)
                     /locus_tag="VFV99_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059298.1"
                     /translation="MRNRLRHRRPDNPQPDNPQPDNPQPDNPHPDNPQPDNPQPDNPQ
                     PDNPHPDNPQPDNPHPDNPHPDNR"
     gene            4021..4194
                     /locus_tag="VFV99_08070"
     CDS             4021..4194
                     /locus_tag="VFV99_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059299.1"
                     /translation="MTVSRLRVAKSETRDMAEHDRIVRSLQTSEWHMSNATFFTAFVV
                     IAAALAVLALIER"
     gene            4307..4993
                     /locus_tag="VFV99_08075"
     CDS             4307..4993
                     /locus_tag="VFV99_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX5059300.1"
                     /translation="MTALRIDVWSDIACPWCYVGKRRLETALAKFPHEVEIVWRSFEL
                     DPSAPKLRDNNNYVERLAAKYRLPVPQAQQMIDRMTRVGADEGLDFRFDRVRAGNTFD
                     AHRVLHLARERGKQAALKERFMRGYFSEGLAIGDREALVAPAVEVGLDTMEVHDALDS
                     DRYADDVRRDEKLAAELGISGVPFFVMAGRLGVSGAQAPDVLLGAVERAREDVAGEQA
                     GGEVCGPEGC"
     gene            complement(5034..6419)
                     /locus_tag="VFV99_08080"
     CDS             complement(5034..6419)
                     /locus_tag="VFV99_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HEX5059301.1"
                     /translation="MVRAAIVPILLSAAAAHAGVVNVSTPAELTAAITSAQAGDEIVL
                     ASGTYALTGATCSAVGTAAQPIIVRSATPLGAKIEFDALEGFKVTGAHWHFEGLDVRG
                     VCASDPNCEHAFHVMGAADGFVLRNSRVVDFNAQLKVNVSMIGSTYVMPNNGLVEYNE
                     VGDTRGRNTSNPVTKLNIDTGNDWIVRGNYLHDAYKLQGDNTSYAAFMKSGGKRGLFE
                     RNLVICSRDTTGGTRIGLSFGGGGTAPQFCAPAYDINTPCSVEHDGGTMRNNIIVNCS
                     DVGIYLNRGKDSHILFNTLIATSGVDFRFDTTSGEAVGNLLAGMIRARDLAMLSASGN
                     TMNVAMTQFTSWYQAPLTGDLRPVGDITSLIGSAPMRTDVLDDYCAQLRPAGALTMGA
                     IEHSVGTCDTTRPPMGTPVGPYGDDVPHGDDSPTNDPPGGGGGCCQAPGHPDGLPVLF
                     VLIVLARRRRA"
     gene            6552..6827
                     /locus_tag="VFV99_08085"
     CDS             6552..6827
                     /locus_tag="VFV99_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059302.1"
                     /translation="MAEIETEIEAPSLDLTVRLDRPFHELKEYLLAEFERAYLLRCLG
                     ESEMNVSLASRNSGLSRKHLRTLMAKYGIRRGEPLGENDNEADSDIV"
     gene            6954..7109
                     /locus_tag="VFV99_08090"
     CDS             6954..7109
                     /locus_tag="VFV99_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059303.1"
                     /translation="MRSERLNVAASLAKLGAAPATAAFILQFTDFFVSLYENAGDGAT
                     VGIAING"
     gene            complement(7099..7341)
                     /locus_tag="VFV99_08095"
     CDS             complement(7099..7341)
                     /locus_tag="VFV99_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059304.1"
                     /translation="MNTVVGGAEQAPIDDEGPQQRTWGQVGREYAAACVQGAGQALMY
                     GGMPRSKKEAAMTAAFGCATGVGTKAIDDLAGAISR"
     gene            complement(7498..>7780)
                     /locus_tag="VFV99_08100"
     CDS             complement(7498..>7780)
                     /locus_tag="VFV99_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059305.1"
                     /translation="LLDVLSLYGGGGLVLTTGDSTITAQLDSQLSINADMLPVGNATI
                     TGSGDSGPSAATVHALVGAMIHTRHARIFLQGAFAPDELSVSLGLRMVP"
BASE COUNT         1207 a         2493 c         2586 g         1494 t
ORIGIN      
        1 aatccggcca attacgcgct agaaccctct cgtgatcggc gagctggtca tgggcgtcta
       61 cgacggcctg cgcggagcgg cgtacttggc gacccacccg cggctatgga agtgggtgct
      121 cgcgcccgcc atcgtcgcgg cactgctgct gttcgtggtg atcggcacga cgctctcggc
      181 actgtcgacc ccgatcgcgg ctatcgctgc gttcttgccc gggagttggg ccgacaacct
      241 gctggagctc gcggccggca tcgtgctcac gatcgccagc ttctcgatct tcatctcggt
      301 cgccgccctg atcgcgggtc ccttcaacga ggagctgtcg gagtcggtcg aggagcacgt
      361 gaccggcgta cctggcccga agttcagcat cgggcgtttc ctcgtcgatc ttctgatcgg
      421 catcatccac gccgcgcgca gggtcttcgt ctacctggtc gtgatgggcg tgctgctcat
      481 cgtcggcgtc gtggtgcccg gcatcggcac ggtgatcgcg gcggtgctcg gcttcatcgc
      541 gaccgcgcgg ttcgcgtcgt acgacgcgtt cgacgcgatc tggtcacgcc ggcgctatcg
      601 ctatcgccag aagactggct acttgcgaga acaccgctgg cgcacactcg gcctcggagc
      661 cgtgtgcgcg gtgccgctcg tcgtgcctgg tctcaacgtg atcgctctcg cgatcggcgc
      721 gacggcggcg acgctgcgcg tggtcgaaca agaacgacta cgcgcagcgg ttggtgctgc
      781 taccaaatcc tgacgcgctt gttcggagcc aggtagagct tgtcgcccgg ctggatcttg
      841 aacgcgtcgt accaggcgtc gaggttacgc acgaccgacg tgcggaactc cgacgggctg
      901 tgcgggtcgg tgacgaggcg ctgcatcagc tcctgatcgc gatagaggcg acgccacgcc
      961 tgggcccagc cgaggaagaa gcgttgatcg ccggagaggc cgtcgatcac gggcgcgtcc
     1021 ttgccgttga gcgagagctt gtacgcctgg tacgcgatcg tgagaccggc gaggtcggcg
     1081 atgttctcgc cgagcgtcag ctcacccttc acacactgcg cgggcttgcc gccagcggcc
     1141 gggaacgggc agtaggtgtt gtactgcgcg acgagctgcg cggtcgcagc cttgaacttc
     1201 gtctcgtcgt ccttcgtcca ccagttctcg agcttgccgg tcgcgtcgta ctggctgccc
     1261 tgatcgtcga agccgtggct gatctcgtga ccgatcacgg caccgatgcc gccgtagttc
     1321 acgccgtcgt ccgcgttcgg atcgaagaac ggcggctgga ggatcgcagc cgggaacacg
     1381 atctcgttga gcgccgggtt gtagtaggcg ttcaccgtca tcggcgtcat gccccactcg
     1441 tcgcgatcga cgggcttgcc gagcttcgcg agacggcggt cgtactcgaa cgccgaggcg
     1501 cgatcggcgt taccgaacgc atcgccggcc ttgatcgtca gcttcgagta gtcgcgccac
     1561 ttcttcggat aaccgatctt cgggttgtac gtggcgagct tcgccttcgc cttcgttttc
     1621 gtctccgggc ccatccacgc gagcgcgtcg agacgttggc ccatcgccgt caacagattc
     1681 ttcacgagct gatcggcctg cgccttcgtc gccggagtga agtacttctc gacgtagagc
     1741 ttgccgacgg cctcgcccat cgtgccggtg atctgatcga caccgcgctt ccagcggtcc
     1801 ttgatctgcg gcgtgcccga cagcgtcttg ccatacatct cgaagtgcgt gtcgacgaac
     1861 gccttcggaa gatacggtgc cgcttcgccg agcatgatga ccgtcaggta gtccttccag
     1921 actccgatcg gctcgctcgc gacgagcttc gcagtgcccg cgatcgccga cggttgattg
     1981 acgttgacct ctttgacctt ctcgaggccg gtcgccttga gccacgcctt ccaatcgatg
     2041 tcgggcgccg tcttctgcag ctcgtcgatc gtgagcttgt tgtacgtctt ctgcgggtcg
     2101 cgattctcga tgcgcgtcca gtgcgtcgcc gcgatcttct cttcgagcgc gtaaatgtca
     2161 gcagcgcgct tttcggcgtc cttctcgccg gcgagcgtca gcatcgcagc gatgtacttc
     2221 ttgtaaccct cgcgaagcgg ctcgaactgc gcgttcttcg cgtcgtacat gtcgcgatcg
     2281 ggaaggccga gaccgccctg gccgatgttc gcgatatgcg tctcgggcgc cttgtcgtcc
     2341 tgaccgacga tcgtcgggaa cgggctcgtg cggaagtgcc gcgcgaagta accgaacagc
     2401 ggcacgagct ccttcgcgct cttgaccgct ttgatcttcg cgagctcgcc cttgatcggc
     2461 gcgatgccct tcttctcgat gccagcttca tccatgaagc tcgcgtagta atcggcgatc
     2521 ttcttcgcct cgctgccggg cttgcccttc gcgttgacga tgatctcctt cgtgcgctcg
     2581 tcgctcttgt ccgagaggat gccgaacgtt ccgtagttcg acttgtcctc gggaatcggc
     2641 gtcgtctcga gccacgtgcc gttcgagtag ttgtagaagc tggcgccggg cgcgagcttc
     2701 gtgttcatgc ccttgaggtc gaagccccac gttccgatct cgggcgcgcc acccttctgc
     2761 ggagcgggcg gattgcctgg agttggaacg acgagctcgc cgcccttcgc tgtcgttgtc
     2821 gcgggcgtcg gcgccggagc cgtggtcggg tccgtcgtcg tagtcggcgg cgtcggagca
     2881 gccgtggtgg tcttcggttg cggcgacgag ccacagccag caaacaagat cgcagcgagc
     2941 agtctcttcc tcatgcccgg gtgcgtatca ggcccgcgag agattgccta gatgcgaccg
     3001 cagaatgacg cacccctgac gcacccaggc atcagtctag aagtaaaagt tggctcccaa
     3061 cgcaaacgag gctccggaca aggagtagcg agagagctga tagtcgacgg gctccggtgt
     3121 tcccggaggc agtgctccga gatccttgtt ttctccgacg gcgacgagcc ggacctcgag
     3181 gatcagcgcg aaccagttcc agcggtactc gccgccgatg ccgaggcgca gcgagccacg
     3241 tccctgcctc gcatcatcgc tcgcatcttt gtgagcaact gcgaggacgc cgagccctgc
     3301 gagcccgtag aggttgagcg gctgttcggc gaggaaccgg taccgaaaat cgacgtaaag
     3361 cccgcccgcg ctgatgtcct tgctgcctgc aaggtgcagc gcaaggccga gctcgatcgg
     3421 cgccctgatc cgatagcgcg ctgcgagctc gaactggccg aactcggtcc gctgcgcgtt
     3481 gtcggcgtcg ggcttgtatg actcggggcc gacgtcgacg ccgatggccc accgcttcgc
     3541 catcaccgat cgacgcgcag gtgccggctg catcgcgggt ggctgctgtc cgggatacgg
     3601 ctgcatcacc ggagcgggtt gtccggctac cggttgtccg ggtgcgggtt gtccgggtgc
     3661 gggttgtccg gctgcgggtt gtccgggtgc gggttgtccg gctgcgggtt gtccggctgc
     3721 gggttgtccg gctgcgggtt gtccgggtgc gggttgtccg gctgcgggtt gtccggctgc
     3781 gggttgtccg gctgcgggtt gtccggccgg cggtggcgga ggcgattgcg catgcgccac
     3841 tgaaccgagc gctacaacga acaacagcgc acgaagcccc atcacttcat tatcgatcat
     3901 ctctgtcccg tgaggggcct tatcagcgaa gtcgctcgag aaccggttcg ctgtggcagg
     3961 acaccgatac ggacggcaag aacttggaac ctcgcgcgcg tgtgatccac acttcgccgc
     4021 atgacagttt cccgtcttcg ggtcgcaaaa tccgagacgc gcgatatggc cgagcacgac
     4081 cgtatcgtgc ggtcgctcca gacgtccgag tggcacatga gcaacgcgac gttcttcacc
     4141 gcgttcgtcg tgatcgcggc ggcgctcgcg gtcctcgcgc tgatcgagcg ctgatcgtgc
     4201 gctgatcgtg cgctgatcgt ccgctgatcg tgcgctgatc gtgcgctgtc gctcgtcagc
     4261 gagcgccgca gcggtgtcgg tgcgacgtcg gtgctagggt gtgagcgtga cagcgcttcg
     4321 catcgatgtt tggtccgata tcgcgtgtcc gtggtgctac gtcggcaagc ggcggctcga
     4381 gaccgcgctc gcgaagtttc cgcacgaggt cgagatcgtg tggcgatcgt tcgagctcga
     4441 tccgagcgcg ccgaagctgc gcgacaacaa taactacgtc gagcggcttg ccgcgaagta
     4501 ccggctccca gtcccgcaag cgcaacagat gatcgatcgg atgacgcgcg tcggtgccga
     4561 cgaagggctc gacttccgct tcgatcgcgt gcgagccggc aatacgttcg acgcgcatcg
     4621 cgtgctccat ctcgcgcgcg agcgcggcaa gcaagcggcg ttgaaggagc gtttcatgcg
     4681 cggctacttc agtgaaggct tggcaattgg cgatcgcgaa gcgctggtcg cgcccgccgt
     4741 cgaggttggc ctcgacacga tggaagtgca cgacgcgctc gatagcgacc gctacgccga
     4801 cgatgtgcga cgcgacgaga agctcgcagc cgagctcggc atcagcggcg tgccgttctt
     4861 cgtcatggcg ggacggctcg gcgtctcggg cgcgcaagca cccgacgtac tgctcggtgc
     4921 agtcgagcgc gcccgcgaag atgtcgctgg cgagcaagcc ggcggcgagg tctgcggtcc
     4981 cgaaggctgc tagctcgctc gcggtcggcg cgtcgtaaag tcgccgagcg aaatcacgcg
     5041 cgtcggcgtc gcgcgaggac gatcagcacg aacagcaccg gaagcccgtc gggatgaccc
     5101 ggcgcctggc agcagccgcc gccaccgccg ggaggatcgt tggtcggcga atcatcgccg
     5161 tgcggaacgt cgtcgccgta cggaccgacg ggcgtaccca tcggcggccg cgtcgtatcg
     5221 caggtgccga ccgagtgctc gatcgcgccc atcgtcagcg cacccgcggg acgcagctgc
     5281 gcgcagtaat cgtcgagcac atcggtgcgc atcggcgcgg agccgatcag cgacgtgatg
     5341 tcgccgactg gcctgaggtc gccggtgagc ggcgcctgat accagctcgt gaactgtgtc
     5401 atcgcgacgt tcatcgtgtt gccgctcgcg ctgagcatcg cgaggtcgcg cgcgcggatc
     5461 atgcccgcga gcaagttgcc gacggcctcg ccgctcgtcg tgtcgaagcg aaagtcgacg
     5521 ccgctcgtcg cgatcaacgt gttgaacagg atgtgcgagt ccttgccgcg gttgagatag
     5581 atgccgacgt cgctgcagtt gacgatgatg ttgttgcgca tcgtgccgcc gtcgtgctcg
     5641 acgctgcacg gcgtgttgat gtcgtacgcg ggcgcgcaga actgcggcgc ggtcccgccg
     5701 ccgccaaacg agaggccgat gcgcgtgccg cccgtcgtgt cccgcgagca gatcacgaga
     5761 ttgcgctcga acaggccgcg cttgccgccg ctcttcatga acgcggcgta gctcgtgttg
     5821 tcgccttgca gcttgtacgc gtcgtggagg tagttgccgc gcacgatcca atcgttgccg
     5881 gtatcgatgt tgagcttggt gaccgggttc gacgtgttgc ggccgcgcgt gtcgcccacc
     5941 tcgttgtatt cgacgaggcc gttgttcggc atcacgtacg tcgagccgat catcgagaca
     6001 ttgaccttga gctgcgcgtt gaagtcgacg acgcgcgagt tgcgcagcac gaacccatcg
     6061 gccgcgccca tcacgtgaaa tgcgtgctcg cagttcggat cactggcgca cacgccgcgc
     6121 acgtcgagac cttcgaagtg ccagtgcgca ccggtgactt tgaagccttc gagtgcgtcg
     6181 aattcgatct tggcgccgag cggcgtcgca gaacggacaa tgatcggctg tgcggcagtg
     6241 ccgacggcag agcaggtcgc gccggtcagc gcgtaagtgc cgctcgcgag cacgatctcg
     6301 tcgcccgcct gcgcgctcgt gatcgcagca gttagctccg ctggagtgga gacgttgacc
     6361 acgccggcat gcgcggccgc ggcactgagc aaaattggaa caatcgccgc gcgcaccata
     6421 tgatctagtc tatccgcgta cttgcatggt gcgtgaagat tctcgcaacc gagtacacct
     6481 acagccccac ttgctctcac atggacggac tacctcgttt ccgaagtgcc cttggaacgg
     6541 ttccccccga gatggcggag atcgagaccg aaatcgaggc gccgagcctc gatcttacgg
     6601 tgcgcctcga tcgccccttc cacgagctca aggagtacct gctcgcggag ttcgagcgcg
     6661 cctacctcct gcgctgcctg ggcgaatccg agatgaacgt ctcgctcgcg agccgcaact
     6721 cgggcctgtc gcgcaagcac ctgcggacgc tgatggcgaa gtacggcatt cgtcgtggcg
     6781 agccgcttgg cgagaacgac aacgaagccg acagcgacat cgtctagtca gtcgcgcgtg
     6841 accgccgagc gtccaaacgg cgcgaggcag ctgaacacga tcgtctggac gaagcgcggg
     6901 ggcttcgcga gtcggaattc gcgacgggtc acgacgcgaa acgcgtgcag cagatgagga
     6961 gcgagcggct caacgtcgcc gcgagtctcg cgaagctcgg cgcagcgccc gcgactgcag
     7021 cgttcatcct gcagttcacg gacttcttcg tctcgctgta cgaaaatgcc ggcgacggcg
     7081 ctacggtcgg tatcgcgatc aacggctgat cgcgcccgcg agatcgtcga tcgccttcgt
     7141 gccgacgccc gtcgcgcaac cgaacgccgc ggtcattgcc gcttccttct tgctgcgcgg
     7201 catgccgccg tacatgagcg cctgtccggc accctgcacg cacgcggcgg cgtactcgcg
     7261 gccgacttgg ccccacgtgc gctgctgcgg cccttcgtca tcgatgggcg cctgctcggc
     7321 gccgccgact acggtattca acgtttcagt ggtgatgact tcgaattcct gcatgccctc
     7381 gcgtagtgca gtgcgagtgc catgcgcgtc gtcgcagatc gcgcggactt gacgcgatca
     7441 cgctggaaga tcgagttgcg atcgctgtac gggactctgc ccctcgacgg tgtctagtca
     7501 cggcaccatg cgtaaaccga ggcttacaga cagctcgtcg ggcgcgaacg cgccttggag
     7561 aaagatgcgt gcgtgacgcg tgtggatcat cgcaccgacg agcgcgtgca cggtggcggc
     7621 gctcggcccg ctgtcgccgg agccagtgat cgtcgcgttg ccgacgggca gcatgtcggc
     7681 attgatgctg agctggctgt cgagctgcgc cgtgatcgtg ctgtcgccgg tggtgagcac
     7741 gagcccgccg ccgccgtaga gtgagagtac gtcgagcagg
//
LOCUS       DASUFX010000206         6068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1143142, whole genome shotgun sequence.
ACCESSION   DASUFX010000206 DASUFX010000000
VERSION     DASUFX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143142"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFV99_08105"
     CDS             complement(<1..1013)
                     /locus_tag="VFV99_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059306.1"
                     /translation="MRGRLTFAVAVCFASSIAHAQRGSATSTATAPNADEPHVAAHVV
                     IDLTPAPGVPAWFASSFEQTITRELAGFERLGTVAKQDIAIEGCADRACKLRKYRAAD
                     VDVVLFGTVRDAAIDYELYQTWTPARLEAGEISIGRSQTIVGLEHETRDALHVVLKHG
                     GLLDQKPYAFEHAVADSGADFSWATSQLAIVLAILALLCLPFAWAAFIGRTPRAILRM
                     RSARRVAVAAAAVAVISVEPSAIASLVAAWPAFVAGLGGLAWGAFGVHLARSAFPPLD
                     GLERMPHGDLVRILKTWCLAAAERVVVLVIGYGGLFVLLIWLGDELAVSSLWTGLVIA
                     PAG"
     gene            1076..2389
                     /locus_tag="VFV99_08110"
     CDS             1076..2389
                     /locus_tag="VFV99_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HEX5059307.1"
                     /translation="MERLSRSHADILTSKLARVPRMAGTSMRGGVLALVMNLRKRART
                     EGERESLFRIVASLGKLKGAAMKMGQHLSYCDPSLPDDVCATLAALQTHSPAMSVSRV
                     TKILHKDLGAAAAPLISSLEPTPIGAASIGQVHRARLADGTRVAVKVQYPDMPSALKA
                     DFGPAAIAGRFAAWVYPAVDVDSYLSEAKARVLDECDYRAEARHHAALASHYADHDTI
                     TIPAIHAEYCGGRVLTTTFVDGKHLDTWLATNPPQTERDAIGLALIDFYVGSALRWNI
                     LPGDPHPGNYVIVAGRRLAIFDHGCTRSFAAARARPVLEAPDLQTALRAAGDVGPEAY
                     LLLRVRFGVAAVLAHLGVRATWHELVTQIMTPKRFEVVLLAPGDRMIEIMREVRDLTG
                     GNIADAKLLVEQTPRVLKTTLDRREAEALKRRLETSGGVVEIRAS"
     gene            complement(2392..3393)
                     /locus_tag="VFV99_08115"
     CDS             complement(2392..3393)
                     /locus_tag="VFV99_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HEX5059308.1"
                     /translation="MSTPGLHHALLLDGKGGARALDWTAVGTWTPADGVLWLNFDYTV
                     SETMQWLATTSQLDAVTISALTDSDPRPRAVAHGDHFLMILRGINNNVGAAPEDMISV
                     RSYIAPQRVLTFHHRSSTTIASILRELEHGTGPRTAGELALVLVERIVEHVVARVDLL
                     GDAVAACEDRTLGEEEARPTRAAGQRADLRHTLADQRRRAIQLRRFLAPQRDAMHRLA
                     SLNVPLLEPQHRARVSEVSEDMLRAIEELDAARDRAAVTQEELSSRVADTANQRLYVL
                     SIITAFFLPLGFVCALLGVNIGGVPLMHEDWAFWGLCGVFVVYVLVQLWFFRRRGWL"
     gene            3421..4059
                     /locus_tag="VFV99_08120"
     CDS             3421..4059
                     /locus_tag="VFV99_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5059309.1"
                     /translation="MRRAVLLVMIASATAAADRPAAKPAAKPSTKASAKPTDFVEVAT
                     VIPDAVIDMRYATADNFAKQKVYPVAVCKLRRAVATRLAKAAATLRKQKRRLLIWDCY
                     RPTSVQEVFWKLVPDERYVANPKQGSRHSRGAAVDLGLVDKDGNAVTLPTKFDDFSEA
                     AHRENALEGEQGAEARTLEAAMKAAGFVGMPTEWWHYDAPDAAKYPLANDPL"
     gene            complement(4056..6002)
                     /locus_tag="VFV99_08125"
     CDS             complement(4056..6002)
                     /locus_tag="VFV99_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059310.1"
                     /translation="MILSLMFFAACSGDKDLATAQQCNPLGGAACITPWPSSLYEVDD
                     PATTSLTHRRVEVPKGALPKNFDNIELSPDVFNKQDGWSYAAPPMIAFASGVDGSNLV
                     SWKDIGKSITPDSPTVLINLKTGELVPHFAELDAREPDKVASQALYIRPAKLLDPGTQ
                     YAVAIKKTLKAKDGTELDIPEGFQAILDDQTTSHKLLEKIRPRFADIFAALEAKGIHK
                     DDLVVAWDFTTRSRESVQADLYDARTATLAMTGTNGSALSFSVDMDTVPTDTRFARRI
                     DGTFDAPLFLTMNGSFGPNIELVRDSAGKPMPTGLYRVPFTALIPTCAMNATAPVPLM
                     IYGHGLLGKAADQVSSGGPKAAASEVCAVVFGTDMRGMSEQDVPNVALALNDGNLGHL
                     IFDTLIQGMMNHVALVQIARGPMATQLFTKTPGGATLVDPSRVYYYGISQGGIFGGTV
                     CGIDPVIKKCVLQVNAINYSMMLERSVDWPTYRTTLIGAYDDPLVVALMLNLMQQQWD
                     RTDPVVVGDVLTTTGFPDTPAKQVLMQFGIADDQVPNVASEFQLRSMGLPIITPTPYT
                     PYGATEAASAPSGVVIYDFGLGNTIPVGNEPPPSNTVHSNIRNKKATTDMMKAFYETG
                     TITNLCTAPKGCDCTVADACGAAI"
BASE COUNT         1012 a         1914 c         2140 g         1002 t
ORIGIN      
        1 aaccccgcgg gtgcgatcac gaggccggtc cacagcgacg agaccgcgag ctcgtcgccg
       61 agccagatga gcagcacgaa caggccgccg tatccgatca caagcacgac gacgcgttcg
      121 gccgccgcga ggcaccatgt cttcaagatc cgcacgaggt cgccatgggg catgcgctcg
      181 agcccatcga gcggcgggaa cgcactgcgc gcgaggtgga cgccgaatgc tccccaggcg
      241 aggccgccga gtcccgcgac gaacgcgggc catgctgcga cgaggctggc gatcgcgctt
      301 ggctcgacgg agatcaccgc gaccgcggcg gctgcgacgg caacgcgccg tgcggatcgc
      361 atgcggagaa tcgctcgggg cgtgcggccg atgaacgcgg cccaggcgaa cggcaggcag
      421 agcagggcga ggatcgcgag cacgatcgcg agctgactcg tcgcccagct gaagtccgcg
      481 ccgctgtcgg cgaccgcatg ctcgaacgca tacggcttct gatcgagcag gccgccatgc
      541 ttgagcacga cgtgcagtgc atcgcgcgtt tcatgctcga ggccgacgat cgtctgcgac
      601 cgaccgatgc tgatctcgcc ggcttcgagt cgtgccggcg tccacgtctg atagagctcg
      661 tagtcgatcg ccgcgtcgcg caccgtgccg aacagcacga cgtcgacgtc tgccgcgcga
      721 tacttgcgta gcttgcacgc gcggtccgca caaccttcga tggcgatgtc ctgtttcgcg
      781 accgtgccga gccgctcgaa gcccgcgagc tcgcgcgtga tcgtttgctc gaagctgctc
      841 gcaaaccacg ctggcacgcc gggagcggga gtgagatcga tgacgacgtg cgccgccacg
      901 tgcggctcgt cggcgttggg cgctgtcgct gtcgacgtcg ctgaaccgcg ttgtgcgtgc
      961 gcgatcgacg acgcgaagca gaccgcgacg gcaaacgtca gccggcccct cacgccagca
     1021 tggtactaga ggctgcatgg accacaacag tcgggagcga cgaaggtgcg agaccgtgga
     1081 gcgcctatct cggtcgcatg ccgacatcct cacgtcgaag ttggcacgcg ttccgcgcat
     1141 ggccgggacc tcgatgcgtg gtggtgtcct cgcgctcgtg atgaaccttc gcaagcgagc
     1201 gcgcaccgag ggtgagcgcg agtcgctatt tcgtatcgtc gcgtcgctcg ggaagctcaa
     1261 gggcgccgcg atgaagatgg gacagcattt atcgtattgc gatccgtcgt tgcccgacga
     1321 cgtgtgcgca acgcttgctg cgctgcaaac gcactctcca gcgatgtccg tatcgcgggt
     1381 caccaagatc ctgcacaaag atctcggcgc cgccgccgcg ccgctgatca gctcgctcga
     1441 accgactccg atcggtgcgg catcgatcgg acaggtccat cgcgcccggc ttgctgacgg
     1501 cacccgcgtc gcggtgaagg tgcagtaccc ggacatgccg agcgcgctga aggcggactt
     1561 cgggcccgcc gcgatcgcgg gccgcttcgc agcgtgggtc tatcccgcgg tcgatgtcga
     1621 cagctatctg agcgaagcga aggcgcgtgt gctcgacgag tgcgattacc gcgccgaggc
     1681 gcgccatcac gccgcgctcg cgagccacta cgcggatcac gacaccatca cgatcccggc
     1741 gatccacgcc gagtactgtg gcgggcgcgt cctcacgaca acgttcgtcg acggaaagca
     1801 cctcgacacc tggctcgcga cgaaccctcc gcaaaccgag cgcgatgcga tcggcctggc
     1861 attgatcgac ttctacgtcg gctcggcgct gcgctggaac atcctgccgg gcgatccgca
     1921 cccgggaaat tacgtgatcg tggctggccg ccggctggcg atcttcgatc acggctgcac
     1981 gcggtcgttt gctgcggcac gagcgcggcc cgttctcgag gcacctgatc tgcagaccgc
     2041 gctgcgtgcg gcgggcgacg ttggcccgga ggcatatcta ttgttgcgcg tacgcttcgg
     2101 cgtcgctgcc gtgctggcgc atctcggcgt tcgcgcgaca tggcacgagc tcgtcacgca
     2161 gatcatgacg ccgaagcgct tcgaggtcgt gctgctcgcg cccggcgatc gcatgatcga
     2221 aatcatgcgc gaggttcgtg atctcaccgg cggcaacatc gcggacgcga agctgctcgt
     2281 cgagcagacg ccgcgcgtgc tcaagacgac gctcgaccgc agagaggccg aagcgctcaa
     2341 gcgcaggctc gagacctccg gcggcgttgt cgagattcgc gcgagctgaa atcacagcca
     2401 gccgcggcgg cggaagaacc agagctgcac gagcacgtag acgacaaaca cgccgcacag
     2461 tccccagaac gcccagtcct cgtgcatgag cgggacgccg ccgatgttga cgccgagcag
     2521 cgcgcacacg aatccgagcg gcaggaagaa cgccgtgatg atcgaaagca cgtagaggcg
     2581 ctggttcgcg gtgtcggcga cgcgcgacga cagctcctct tgcgtgacgg cagcgcggtc
     2641 gcgtgcggca tcgagctctt cgatcgcccg cagcatgtct tcgctgacct cggagacgcg
     2701 tgcgcggtgt tggggttcga gcaatggcac gtttagcgag gcgaggcggt gcatcgcgtc
     2761 gcgctgcggt gcgagaaagc ggcggagctg gatcgcgcgg cgtcgctgat cggcgagcgt
     2821 gtggcgaagg tccgctcgct gacctgcggc gcgggttggc cgcgcttcct cttcgccgag
     2881 cgtacggtct tcgcaggccg cgaccgcgtc acccagcaga tcgacgcgcg cgacgacgtg
     2941 ctcgacgatg cgctcgacga gcacgagggc gagctcgccc gcagtgcgcg ggccggtgcc
     3001 gtgctcgagc tcgcgcagga tggaggcgat cgtggtcgag gagcggtggt gaaacgtgag
     3061 tactcgctgc ggcgcgatgt acgagcgcac ggaaatcatg tcttcgggcg cggcaccgac
     3121 gttgttgttg atgccgcgca ggatcatgag gaagtgatcg ccgtgtgcga ccgcgcgcgg
     3181 gcgcggatcg gagtcggtga gcgcggagat cgtgacggcg tcgagctggg aggtggtggc
     3241 gagccactgc atggtctcgc tgaccgtgta gtcgaagttg agccacagca cgccgtcggc
     3301 gggtgtccag gtaccgaccg cggtccagtc gagcgcccgc gcaccgccct tgccgtcgag
     3361 caggagcgcg tggtggaggc ccggtgtgga catgcgagct tgttgtacta cgctcgatgt
     3421 gtgcggcgcg cggtgttgct cgtgatgatc gcgtcggcga ccgcggctgc cgatcgcccg
     3481 gctgcgaaac cggccgcgaa accgagcacg aaggcgagcg ccaagccgac cgacttcgtc
     3541 gaggtcgcga cggtgattcc cgacgccgtc atcgacatgc ggtacgcgac cgccgacaac
     3601 tttgcaaagc agaaggtcta cccggtcgcc gtctgcaagc tgaggcgggc ggtcgcgacg
     3661 cggctcgcga aggcggcggc cacgctgcgc aagcagaaac ggcggctcct gatctgggac
     3721 tgctaccgcc cgacgtcggt gcaggaggtg ttctggaagc tcgtgcccga cgagcgctat
     3781 gtcgcaaatc ccaagcaggg gtcgcggcac agtcgcggcg ccgcggtcga cctcgggctc
     3841 gtcgacaagg acggcaacgc ggtgacgcta ccgacgaagt tcgacgattt ctcggaagcc
     3901 gcgcaccgcg agaatgcgct cgaaggcgag cagggcgccg aggcgcgcac gctggaagcg
     3961 gcgatgaaag cggccggctt cgtcggcatg ccgaccgagt ggtggcacta cgacgcgccc
     4021 gacgccgcga agtatccgct cgcgaacgac ccgctctaga tcgcagcgcc gcaggcgtcg
     4081 gctaccgtgc agtcgcagcc cttgggcgcg gtgcacaggt tcgtgatcgt gccggtctcg
     4141 tagaaggcct tcatcatgtc ggtcgtcgcc ttcttgttgc ggatgttgct gtgcacggtg
     4201 ttcgacggcg gcggctcgtt gccgaccggg atcgtgttgc cgaggccgaa atcgtagatc
     4261 acgacgccgc tcggcgcgga tgcggcttcg gtagcgccgt acggcgtgta cggcgtcggt
     4321 gtgatgatcg gcaagcccat cgagcgcagc tggaactcgc tcgcgacgtt cggcacttga
     4381 tcgtcggcga tgccgaactg catgagcacc tgcttggcgg gcgtgtcggg gaagccggtc
     4441 gtcgtgagca cgtcgccgac gacaaccggg tcggtgcggt cccactgctg ctgcatgagg
     4501 ttgagcatga gcgcgacgac cagcggatcg tcgtaggcgc cgatcagcgt ggtgcggtag
     4561 gtcggccaat cgaccgagcg ctcgagcatc atcgagtagt tgatggcgtt gacctgcagc
     4621 acgcacttct tgatcaccgg atcgatgccg cagaccgtgc cgccaaagat gccgccttgc
     4681 gagatgccgt agtaatagac gcggctcgga tcgacgagcg tggcgccgcc cggcgtcttc
     4741 gtgaacagct gcgtcgccat cgggccgcgc gcgatctgga cgagcgcgac gtgattcatc
     4801 atgccctgga tgagcgtgtc gaagatcaga tggccgaggt tgccgtcgtt gagcgcgagc
     4861 gcgacgttcg gcacgtcttg ttcgctcatg ccgcgcatgt cggtaccgaa cacgacggcg
     4921 cagacttcgg atgccgccgc cttcggaccg cccgacgaga cttgatcggc ggctttgccg
     4981 agcaggccgt ggccgtagat catgagcggt accggcgccg tcgcgttcat cgcgcaggtc
     5041 ggaatgagcg cggtgaacgg cacgcggtag aggccggtcg gcatcggctt gcccgcgctg
     5101 tcacgcacga gctcgatgtt cgggccgaag ctgccgttca tcgtgaggaa cagtggcgcg
     5161 tcgaacgtac cgtcgatgcg gcgcgcgaag cgcgtgtcgg tcggcaccgt gtccatatcg
     5221 accgagaacg acagcgcgct gccgttcgtg ccggtcatcg cgagcgtcgc ggtgcgtgcg
     5281 tcgtagagat cagcttgcac cgactcgcgc gaccgcgtcg tgaaatccca cgcgacgacg
     5341 aggtcgtcct tgtggatgcc cttcgcttcg agcgcggcga agatgtcggc gaagcgcggg
     5401 cggatcttct cgagcagctt gtggctcgtg gtctggtcgt cgaggatcgc ctggaagccc
     5461 tcggggatgt cgagctcggt gccgtccttg gccttgagcg tcttcttgat cgcgacggcg
     5521 tactgcgtgc cgggatcgag gagcttggcc gggcggatgt agagcgcctg gctcgcgacc
     5581 ttgtcgggct cgcgcgcgtc gagctcggcg aagtggggga cgagctcgcc ggtcttgagg
     5641 ttgatgagga cggtcgggct gtcgggcgtg atgctcttgc cgatgtcctt ccacgagacg
     5701 aggttcgaac cgtcgacgcc cgacgcaaac gcgatcatcg gcggcgcggc gtacgaccaa
     5761 ccgtcttgct tgttgaagac gtcaggtgac agctcgatgt tgtcgaagtt cttcggcagc
     5821 gcgcccttcg gaacttcgac gcggcgatgc gtgaggctcg tcgtcgccgg atcatcgact
     5881 tcgtaaagcg acgatggcca tggcgtgatg cacgcagcgc cgccaagcgg gttgcactgc
     5941 tgcgcggtcg cgaggtcttt gtcaccgctg catgcggcga agaacataag cgagaggatc
     6001 aaatactgac gcatggtcgt taccccggcg cagactaacg gaagagggta ctgaagtggc
     6061 ggcatttc
//
LOCUS       DASUFX010000207         8540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1561056, whole genome shotgun sequence.
ACCESSION   DASUFX010000207 DASUFX010000000
VERSION     DASUFX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561056"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1416)
                     /locus_tag="VFV99_08130"
     CDS             complement(250..1416)
                     /locus_tag="VFV99_08130"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5059311.1"
                     /translation="MALGGATALPYFHLGSFDVGVPIQAFGVIVAIGVLIGAGVLRRY
                     AEWHGVSDDHIRGITGWVTVAGFIGAHVFDVLFYQWNDLQENPILLLQIWKGISSYGG
                     FLGGAIGWWIYVWWKRLDMRLLADVTVLGLLVAFSIGRIGCTVVSDHIGAAVDPSQWY
                     AALAVDYPRHVGGLGEHYTAITGPTIRAWNLGLLEFLYLVPVNIFMLWYAFRSTKRPN
                     AGMITALTGLFYAPVRFLLDFLRFNPEADPRYLGLTFAQWVSILAFGASVALVMKLAK
                     NGKPAETVGPTSGEVQRRLKLILKEDDAEKKTKDDDDKKSAPKATAKEAEKSEDDDED
                     EELAKEAEAAGGEGDDDEEDERPAPGKPTTTKPGGNRPRPQGGGSSKKNKKKRR"
     gene            1478..1678
                     /locus_tag="VFV99_08135"
     CDS             1478..1678
                     /locus_tag="VFV99_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059312.1"
                     /translation="MIWGPFLLALALGLLVSAGLYFAYLWPQVEPEEPSATPASAGDP
                     KESAPEQAATEDPEPTPPSESA"
     gene            1675..3048
                     /locus_tag="VFV99_08140"
     CDS             1675..3048
                     /locus_tag="VFV99_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059313.1"
                     /translation="MMYRVLVAATALAIAQPAKGAPKGGVVQVEHRDPSALPSKGPLN
                     ALVTVEVFFTPGQSSRVQAYRNLERLQASHPSRVRLVYRIVTSGGQTRTPYAALQAHA
                     EGKFFEFMDAINATRDSRLADKDLFELAKKVGMDPDRLAQAIARPPRAYDDVLEANEL
                     RRKQRIHGSPQLPSVLFNGRVPTQPLNAGPDLEREYRLAKDYALELLDRGADRGSLSE
                     ALDELAEPTQDIIVSPGATDEDLGDDPGDPVLASPPLDLRGLPSYGPGEAATTIVVLC
                     SPTSQNCVQPMRAARNIRDWYPESVRVVWAPYFDLTREDAAELSLLADGALCAEKIGV
                     TLEERDATFGNAASPGWRWVEAVRSENKSRRVTPDKILDKVAEKLRVDRRAFATCRAQ
                     VAGTSIAWIEAARRAGVRVTPSTIVGGRIYGPITDHNTLQLLIEAELAPGILGEAAPT
                     WRGDSAR"
     gene            3081..4382
                     /locus_tag="VFV99_08145"
     CDS             3081..4382
                     /locus_tag="VFV99_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5059314.1"
                     /translation="MEVSFAAATDVGRQRNHNEDNFLIDKKLRLFMVADGMGGHAAGE
                     VASSIAVHEIRDAINNNKDLIDRYRVDHPGVQAYEILQVLEHAVQAACGAVHSRAQAE
                     PDKRGMGTTATVLLIAGTGEQLRGFIAHVGDSRVYLARQTQCHVLTEDHSLMNELVRR
                     GKLNREQIESSPYKQYKNAVTRAVGVYGSVEVDTFDFDILPGDRFLICSDGMYAYVDE
                     AELPKQLADGDVKEVPKALVDKANKGGGHDNITAVVIRISESAGATATPRTTEIALKL
                     EVLKGMQMFRYLSYKELVTVAAVAEMSEHKAEDKIFEVGAPGDAMYIVTAGSVKLVIG
                     DKIVSELGRGQHFGEMALVDRTTRSLTAISASDETRLVVIHRKEFYDLIKREPGSAVK
                     MLWAFVQVLGQRLRKTNSDLSEALHGDKRGETTERDNQSQE"
     gene            4524..4697
                     /locus_tag="VFV99_08150"
     CDS             4524..4697
                     /locus_tag="VFV99_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059315.1"
                     /translation="MEEYAQLLARAFNGAITVNGQRIVAHPSGTLIVRGSEPRPVVTA
                     IKPLDADNDWSNV"
     gene            complement(4790..5215)
                     /locus_tag="VFV99_08155"
     CDS             complement(4790..5215)
                     /locus_tag="VFV99_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5059316.1"
                     /translation="MSAARPFKVLGIQQIAIGGPSKSLLRHLWIDLFGLEQTGSFKSE
                     RENVDEDILEIAGVEIDLMQPIDPEKKPKVHEPRLNHIGLWVDNLAEAVKWLEGQGVH
                     FTPGGIRKGAAGYDVCFIHPRSAEGVLIELVQHPEHLAD"
     gene            complement(5230..6522)
                     /gene="ccrA"
                     /locus_tag="VFV99_08160"
     CDS             complement(5230..6522)
                     /gene="ccrA"
                     /locus_tag="VFV99_08160"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX5059317.1"
                     /translation="MMMSTAIVSRSSDVYELGDVPELGHLPSKMLAQVIRAERFGEPR
                     DAFKEEALPIPELGPRDVLVYVMAAGVNYNNVWAAMGIPVDVIKSRQKKGEKEDFHIG
                     GSDASGVVWAVGKDVKNVKVGDDVVVHCGMWEPDDPWVKSGKDPMFAPSQIIWGYESN
                     WGSFAQFTKVQDHQCLPKPPQLTWEAAAAYMLVGATAYRMLHGWPPNVVGPGEPVLVW
                     GGAGGLGSMAIQIARAAGARPVAVVSGDSKNEFCKKLGAVGVIDRRKFDHWGKLPRWS
                     DDIEYAQWLKGARAFGKAFWDALGEKKSPTIVFEHPGETTLPTSCFMCETGGMVVICA
                     GTTGYNATLDLRYHWMRQKRLQGSHFANDVQAKGINDLVTAGKVDPCLGRVFRFDETA
                     ECHQLMRDNQHPSGNMAILVNAPRPGLRTLDDVRAAKK"
     gene            complement(6643..7545)
                     /locus_tag="VFV99_08165"
     CDS             complement(6643..7545)
                     /locus_tag="VFV99_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5059318.1"
                     /translation="MAGSRGEVVKALAVNAVIATSKGVAAAITGSGAMLAETLHSFAD
                     CGNQLLLLVGIRQSGKPANQKHPLGYGRAMYFYSFIVAMLLFSGGGLFSIYEGVHKLE
                     HPEPVGDITIALIILAISIALEGWSTLGNIKVLNEKRGTTRFLTYLRETKDSDLVVVF
                     GENSAAVLGLLLATGALLIAKHTNDGRWDAIGSLAIGTVLICVAIFLAREIQSLLVGE
                     SADPKLQKAVEQLAVDDPNVDRVLRLLTVQQGPGEIVVAMKLKFRSGLETTQLVDAIN
                     GFERELKQRVPEVRWSFIEPDHAD"
     gene            complement(7545..8105)
                     /locus_tag="VFV99_08170"
     CDS             complement(7545..8105)
                     /locus_tag="VFV99_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5059319.1"
                     /translation="MSGLSRSRAALLVVDIQDKLAAAMPEDVLAQAVRNTAVLIETAR
                     RMWMPIVVSQQYPRGLGATMPAIEDALRDAPTLHRFDKMIFSAADAPEFGALAPKLGR
                     DQWIVTGMEAHVCVYQSARGLVARGYDVHVPADAITSRTKANFKIARKLIENSGATLT
                     STEVVVFDLLGTAADVEFKALSRLIK"
     gene            complement(8102..>8540)
                     /locus_tag="VFV99_08175"
     CDS             complement(8102..>8540)
                     /locus_tag="VFV99_08175"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059320.1"
                     /translation="RMLRQTYNIKPNEKVAIELRVASDAPRATLEKYKDMIEGRAKVT
                     ATLTSGGDPVPGAAKALVGADVQVLVPLGGLIDVSAETTRIKKEIGKGEKEVAALEKK
                     LGNADFLARAPEEVVAEIRQRLADEQQRMKILVEAIETLGVVK"
BASE COUNT         1477 a         2859 c         2747 g         1457 t
ORIGIN      
        1 gaacatatcc tgcagaatct ttgtgtcttg ctcgctcacg acggggctcc ttgtagtgtg
       61 ccccgcccgt agagcagccg ctatgccatt cacaaacgcg cagaatcgat gtgcgcgctg
      121 tccgagagca tccggcgcgc ggacgcgtcc gacggcgcac ggacagcgga atcgacgtga
      181 cacgttgcga gcgcggacca atcgccgccg ggccgctggt ggtgggctcg acggcgcgac
      241 gctggcaggc tagcggcgtt tctttttgtt cttcttgctc gagccgccgc cctgcgggcg
      301 tggacgattg ccgccgggct tggtcgtcgt cggcttgccc ggcgccggcc tttcgtcttc
      361 ctcgtcgtcg tcgccctcgc cgcccgcggc ttcggcttcc tttgcgagct cttcgtcctc
      421 gtcgtcgtcc tcggacttct cggcttcctt cgcggtggcc ttcggcgccg acttcttgtc
      481 atcgtcgtct ttcgtcttct tctcggcgtc gtcctctttg aggatgagct tcaagcggcg
      541 ctgcacttca cccgacgtcg ggccgacggt ctcggccggc ttgccgttct tcgcgagctt
      601 catgacgagc gcgaccgatg caccgaacgc gaggatcgag acccactgcg cgaacgtcag
      661 cccgaggtag cgcggatcgg cttcggggtt gaagcgcaaa aagtcgagca ggaagcgcac
      721 cggcgcatag aacaggccgg tcagcgcggt gatcatgccg gcgttcggcc gcttcgtgct
      781 gcggaacgcg taccagagca tgaagatgtt caccggcacg aggtagagga actcgagcag
      841 gccgaggttc cacgcgcgga tcgtcgggcc cgtgatcgcg gtgtagtgct cgccgagccc
      901 accgacgtgg cgcggatagt cgactgcgag cgccgcgtac cactggctcg gatcgacggc
      961 ggctccgatg tgatcgctga cgacggtgca gccgatgcgg ccgatcgaga acgcgacgag
     1021 aaggccgagc accgtcacgt cggcgaggag ccgcatgtcg agccgcttcc accacacgta
     1081 gatccaccag ccgatcgcgc cgccgaggaa tccgccgtac gacgagatgc ccttccagat
     1141 ctggaggagc aggatcgggt tctcctggag gtcgttccat tgatagaaca gcacgtcgaa
     1201 cacgtgtgcg ccgataaagc cggcgaccgt gacccagccg gtgatgccgc ggatgtgatc
     1261 gtccgaaacg ccgtgccact cggcgtagcg gcgcaagact cccgcgccga tcaacacgcc
     1321 aatcgccacg atcacgccga acgcctggat cgggacgccg acgtcgaacg agccgaggtg
     1381 gaagtacggc agcgcggtcg cgcccccgag ggccaaagct aaggcgtaca ttgggtcgga
     1441 gcctacaaca gccgccgcct cgcgctatca tcccgaggtg atctggggcc ccttcctact
     1501 cgctttggcg ctcggcctgt tggtgtcggc cggcctgtac ttcgcgtatc tgtggccgca
     1561 ggtcgagccc gaggagccgt cggcaacgcc cgcatcggcg ggtgacccga aagagtccgc
     1621 tccggaacag gccgctaccg aggaccccga gccgacgccg ccgagcgaat ccgcatgatg
     1681 taccgcgtgc tcgtcgcggc gacggcgctc gcgatcgcac agccggcgaa aggcgcgccg
     1741 aagggtggcg tcgtccaggt cgagcatcgc gatccgagcg cgctgccgtc gaaagggccg
     1801 ctcaatgcgc tcgtcaccgt cgaggtgttc ttcacgccgg gccagagctc gcgcgtgcag
     1861 gcctatcgca acctcgagcg gctgcaggcg agccatccgt cgcgggtgcg cctcgtctat
     1921 cgcatcgtga cgagcggcgg ccagacccgc acgccctacg cggcactcca ggcgcacgcc
     1981 gagggcaaat tcttcgagtt catggatgcg atcaatgcga cgcgcgactc gcggctggcc
     2041 gacaaggatc tgttcgagct cgcgaagaag gtcggcatgg atcccgaccg gcttgcgcag
     2101 gcgatcgcgc ggccgcctcg cgcgtatgac gacgtgctcg aggccaacga gctgcgccgc
     2161 aagcagcgca tccacggttc gccgcagctg ccgagcgtgc tgttcaacgg ccgcgtgccg
     2221 acgcaaccgc tcaacgcggg ccccgatctc gagcgcgagt acaggctcgc gaaggactac
     2281 gcgctcgagc tactcgatcg cggcgccgat cgcggctcgc tctccgaagc gctcgacgag
     2341 ctcgccgagc ccacgcagga catcatcgtg tcgcccggcg ccaccgacga ggatctcggc
     2401 gacgaccccg gcgatcccgt cctcgcctcg ccgccgctcg atctgcgtgg cttgccctcg
     2461 tacggtcccg gagaagccgc gacgacgatc gtcgtgttgt gctcgccgac cagccagaac
     2521 tgcgtgcaac cgatgcgcgc cgcccgcaac atccgcgact ggtacccgga gtcggtgcgc
     2581 gtcgtgtggg cgccgtactt cgacctcact cgcgaggacg ccgccgagct gagcctgctc
     2641 gccgatggcg cgctgtgcgc cgagaagatc ggcgtcaccc tcgaggagcg cgacgccacg
     2701 ttcggcaatg cggcgtcacc gggctggcgc tgggtcgagg cggtgcggag cgaaaacaag
     2761 agccggcggg tcacccccga caagatcctc gacaaggtcg ccgagaagct gcgggtcgac
     2821 cgccgcgcgt tcgcgacctg tcgcgcccag gtcgccggaa catcgatcgc ctggatcgaa
     2881 gcggcgcgcc gcgcgggagt ccgagtaacc ccgagcacca tcgtcggtgg ccgcatctac
     2941 gggcccatca ccgaccacaa cacgctgcag ctgctcatcg aggccgagct ggcgccgggg
     3001 atcctcggcg aagcggcgcc gacctggaga ggcgattccg cgcgttgacc ctgttttaac
     3061 ggggtgttac ccagagggtg atggaggttt ccttcgcggc agcgaccgat gtcggtcgcc
     3121 agcgaaatca taacgaggac aacttcctca tcgacaagaa gctgcgcctg ttcatggtcg
     3181 ccgacggcat gggcggtcac gcagcgggcg aagtcgcgtc gtcgatcgcg gtgcacgaga
     3241 tccgcgatgc catcaacaac aacaaagacc tgatcgatcg ctatcgcgtc gaccatcccg
     3301 gcgtgcaggc gtacgagatc ctgcaggtac tcgagcacgc ggtgcaagcg gcgtgcggcg
     3361 ccgtgcattc acgcgcgcaa gccgagcccg acaagcgcgg catgggcacg acagcgacgg
     3421 tgctgctcat cgccggcacc ggcgaacagc tgcgcggttt catcgcgcac gtcggcgaca
     3481 gccgcgtgta tctcgcgcgc cagacgcagt gccacgtgct caccgaagat cactcgctga
     3541 tgaacgagct ggtccggcgc ggcaagctca accgcgagca gatcgagagc tcgccgtaca
     3601 agcagtacaa gaacgcggtc acgcgcgccg tcggtgtcta cggctccgtc gaggtcgaca
     3661 cgttcgactt cgacatcctg cccggcgatc ggttcttgat ctgctccgac ggcatgtacg
     3721 cgtacgtcga cgaggccgag ctaccgaagc aactcgccga cggcgacgtc aaggaagtgc
     3781 cgaaggcgct cgtcgacaaa gcgaacaagg gcggcggtca cgacaacatc accgccgtcg
     3841 tgatccggat cagcgagagc gccggcgcga ccgcaacgcc gcgcaccacg gagatcgcgc
     3901 tcaagctcga agtgctcaag ggcatgcaga tgttccgtta cctctcctac aaggagctgg
     3961 tgacggtcgc tgccgtcgcc gagatgagcg agcacaaagc cgaggacaag atcttcgagg
     4021 tcggcgcgcc cggcgatgcg atgtacatcg tcaccgccgg cagcgtgaag ctcgtgatcg
     4081 gcgacaagat cgtcagcgag ctcggccgcg gccagcactt cggcgagatg gcgctcgtcg
     4141 atcgcacgac gcgttcgcta acggcgatct ccgcgagcga cgagacccgc ctcgtcgtca
     4201 tccaccgcaa agagttttac gacctgatca aacgcgagcc cgggagcgcg gtgaagatgc
     4261 tgtgggcatt cgtgcaagtg ctcggacagc ggctgcgcaa gacgaactca gacctcagcg
     4321 aagcgctgca cggcgacaag cgcggcgaaa cgaccgagcg cgataaccaa tcgcaggaat
     4381 aatcggtccg accggctgct gcgcggtcgc cgttatccac agcgcttcac agcaattcac
     4441 agcaattcac agcaattcac aggtccggag tgtcgttcac ccgtggtacc tgaagcgttg
     4501 tcatgacgat ggggcgacga gtcgtggagg aatacgcaca gctactggcg cgtgcgttca
     4561 atggcgcgat cacggtgaac ggtcagcgca tcgtcgcgca tccgagcggc acgctcatcg
     4621 tgcgcggcag cgagccacgt ccggttgtca cggcgatcaa gccgctcgac gccgacaacg
     4681 actggtcgaa cgtctagccc gtcattcgga cagggtcaga cattacctgc atgcgttcgt
     4741 cggacgagtg cggtccgagc aacgcataca acgcatgtct gaccccgaat tagtcggcca
     4801 ggtgctcggg gtgctgcacg agctcgatca gcacaccttc ggctgatcgc ggatggatga
     4861 agcacacgtc gtaaccggcg gcgcccttgc ggatgccgcc cggcgtgaag tgcacgccct
     4921 gcccttcgag ccacttcacg gcctcggcga ggttgtcgac ccagaggccg atgtgattga
     4981 gccgcggctc gtgcaccttt ggcttcttct cgggatcgat cggctgcatg agatcgatct
     5041 cgacgccggc gatctcgagg atgtcctcgt cgacgttctc gcgctcgctc ttgaacgagc
     5101 cggtctgctc gaggccgaac agatcgatcc acagatgacg aagtagagac ttgctcggac
     5161 cgccgatcgc gatctgctgg atgccgagca ccttgaacgg gcgcgctgcg ctcatcgcat
     5221 cacctcgtgt cacttcttcg ctgcgcgcac gtcgtcgagc gtgcgcaggc cgggtcgcgg
     5281 cgcgttgacg aggatcgcca tgttgcccga cggatgctgg ttgtcgcgca tcagctggtg
     5341 acactcggcg gtctcgtcga agcggaacac gcgaccgagg cacggatcga ccttgccggc
     5401 ggtgacgagg tcgttgatgc ccttcgcctg gacgtcgttc gcgaagtgcg agccctgcag
     5461 ccgcttctgg cgcatccagt gatagcgaag gtcgagcgtc gcgttgtagc cggtcgtgcc
     5521 ggcgcagatc acgaccatgc cgcccgtctc gcacatgaag cacgacgtcg gcagcgtggt
     5581 ctcgcccgga tgctcgaaca cgatcgtcgg gctcttcttc tcgccgagcg cgtcccagaa
     5641 cgccttgccg aacgcgcgcg cgcccttgag ccactgcgcg tactcgatgt cgtccgacca
     5701 gcgcggcagc ttgccccagt gatcgaactt gcggcgatcg atcacgccga ccgcgccgag
     5761 cttcttgcag aactcgttct tgctgtcgcc cgacacgacc gcgaccggcc gcgcacctgc
     5821 ggcacgcgcg atctggatcg ccatcgagcc gaggccaccg gcgccgcccc acacgagcac
     5881 gggctcgccg ggcccgacga cgttcggcgg ccagccgtgc agcatgcgat atgcggtagc
     5941 gccgacgagc atgtacgcag cggcggcttc ccacgtcagc tgcggcggct tcggcaggca
     6001 ctggtgatcc tgcaccttcg tgaactgcgc gaacgaaccc cagttcgatt cgtagcccca
     6061 gatgatctgc gacggcgcga acatcggatc tttgcccgac ttcacccacg gatcatcggg
     6121 ttcccacatg ccgcagtgaa cgacgacgtc atcgccgacc ttgacgttct tgacgtcctt
     6181 gccgacggcc cacaccacgc cgctcgcgtc ggagccgccg atatggaagt cttccttctc
     6241 acccttcttc tgccggctct tgatgacgtc gacggggatg cccatcgcgg cccagacgtt
     6301 gttgtagttg acgcccgcgg ccatcacgta gacgagcacg tcacgtgggc cgagctcggg
     6361 gatcggcagc gcttcttctt tgaaggcgtc acgcggctcc ccgaaacgct cggcgcgaat
     6421 cacttgcgcg agcatcttgc tcggcaggtg gcccagctcc ggaacatctc cgagctcgta
     6481 gacatcgctg gacctggaaa cgatcgcggt cgacatcatc atgacggaat ctactcgatc
     6541 cctctgaaaa tgacgcagtg tcctaacgct gatcttctcc atggttctgc catggagagg
     6601 agcgagcacg cctcagtaga gtgagcgccg aggcggattc gctcagtcgg cgtggtctgg
     6661 ttcgataaag ctccagcgca cttccgggac gcgctgcttc agctcgcgct cgaatccgtt
     6721 gatggcatcg accaattgtg tggtctccag acccgagcgg aacttgagct tcatcgccac
     6781 gacgatctcc cccggaccct gctggacggt gagcaggcgg agaactctgt cgacgttcgg
     6841 atcgtcgacc gcgagctgct cgacggcctt ctgcagcttc ggatcggcgg attcgccgac
     6901 gagcaagctc tggatctcgc gcgcgagaaa gatcgcgaca cagatgagca cggtaccgat
     6961 cgcgagcgaa ccgatcgcat cccaccggcc gtcgttcgtg tgcttcgcga tgagtagcgc
     7021 gccagttgcg agcaagaggc cgagcaccgc cgcgctgttc tcgccgaaca cgacgacgag
     7081 atcgctgtcc ttggtctcgc gcagatacgt caagaatcgc gtcgtgccgc gcttctcgtt
     7141 gagcaccttg atgttgccaa gcgtcgacca gccttcgagc gcgatcgaga tcgcgagaat
     7201 gatgagtgcg atcgtgatgt caccgacggg ctcggggtgc tcgagcttgt gcacgccctc
     7261 gtagatcgag aacaagccac cgccggagaa cagcagcatc gcgacgatga acgagtagaa
     7321 gtacatcgcg cgcccgtagc cgagcggatg cttctggttt gccggcttgc cgctctgccg
     7381 gatcccgacg agcagcagca actggttgcc gcagtcggcg aacgagtgca gcgtctcggc
     7441 gagcatcgcg cccgagccgg tgatcgcggc cgcgacaccc ttgctcgttg cgatgacggc
     7501 gttgaccgcg agcgccttga cgacttcgcc cctggagccg gccattactt gatcaacctc
     7561 gagagcgctt tgaactcgac atcggcggcg gtgccgagca ggtcgaacac gacgacttcg
     7621 gtcgacgtca gcgtcgcgcc ggagttttcg atcagcttgc gtgcgatctt gaagttcgcc
     7681 ttcgtgcggc tcgtgatcgc atcggccggt acgtgcacgt cgtagccgcg ggcgacgagg
     7741 ccgcgcgcgc tctgatagac gcagacatgc gcttccatgc cggtgacgat ccactgatcg
     7801 cggccgagct tcggtgcgag cgcgccgaac tccggcgcgt cggcagccga gaagatcatc
     7861 ttgtcgaagc gatgcagcgt cggtgcgtcg cgcagcgcat cttcgattgc cggcatcgtc
     7921 gcgccgaggc cgcgcggata ctgctggctg acgacgatcg gcatccacat ccggcgcgcc
     7981 gtctcgatca gcacggcggt gttgcgcacg gcctgggcga gcacgtcctc gggcatcgcc
     8041 gcggcgagct tgtcctggat atcgacgacg agcagcgcgg ctcgcgagcg cgacaggccg
     8101 ctcacttcac gactccgagc gtctcgatcg cctcgacgag aatcttcata cgctgctgct
     8161 cgtcggcgag ccgctgccgg atctccgcca cgacttcttc gggcgcgcgc gccaaaaagt
     8221 ccgcattgcc gagcttcttc tcgagcgcag cgacttcttt ctcgcccttc ccgatctctt
     8281 tcttgatccg cgtcgtctcg gcgctgacat cgatcagacc gccgagcggc acgagcacct
     8341 gcacgtcagc gccgacgagc gccttcgcgg cgcctggcac cggatcgcca ccggacgtga
     8401 gcgtcgctgt caccttcgcg cggccctcga tcatgtcctt gtacttctcg agcgtcgcgc
     8461 gcggggcgtc ggaggccacg cgcagctcga tcgcgacctt ctcgttcggc ttgatgttgt
     8521 aggtctggcg cagcatgcgc
//
LOCUS       DASUFX010000208        14661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_866595,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000208 DASUFX010000000
VERSION     DASUFX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14661
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866595"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2544)
                     /locus_tag="VFV99_08180"
     CDS             complement(<1..2544)
                     /locus_tag="VFV99_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5059321.1"
                     /translation="MALSAKRIVRELARPAVFVRILVALAASIGIYMLVDRWIGGRAT
                     LPVDIGSRPITLLDPGKLLLIAIVPFLFLLRVLSLTDLSVLQQVVQATLRSLVIAALA
                     VAAARPSWITETNKVATIVLVDVSDSISDKQLDAAKKYVDELEKAEGNGSLQLITFAE
                     KPLVARAEDDKPLSSAIKRHADGGAGTDTQSAMQLAYGMYPDGYVDRMVIISDGNQTE
                     GDMAIEAYRAKELKVKVSWRTFDADKTAEVRVAGLTLPDDIKVGQPFEVTAELWSTEP
                     QTVTLALQQDEFPNGLEPSKTVELREGKNLVKFKSDAKRAGATTYKVRLSNVAKDTEK
                     KNNVAVMTAPVRGRPNVLYVEGGVMREPGSASYLQKALEHENIDVSVRGPTGIPSNPK
                     ELEKYDLVLVSDVPAHLMGAGQMAALDTYVKGMGGGLIMAGGEDSFGSGGYERTTIEN
                     MMPVRFDSEKIKEQPDIALVLVIDRSGSMQGPKLEAAKESARVTAEVLSPQDYIAVVA
                     FDSEAQVYVRPTRASSKMRISNDIARLQSGGGTNIFPGLKEAFEILQGINAKVKHVIL
                     LSDGEAPTDGLAELVSDMRASRITVSAVGVQGADRNMLAMIADTGEGRLYMVEDIGSL
                     PKIFMKETQEAQKSQLVEDLIHVRVAKKVEAIEGTSVENAPPLHGYVTTKPKPTSETI
                     LISDLGEPILARWRYGSGTSVAWTSDVKNRWSVDWIRWGGYPKFWAQVIRSSMRRKVY
                     DSYDLYAKVADGRAQITVDAIDSGDKFVNELDTELQVVDPANNKVTSTQPMAQTAAGR
                     YTADFKIQKYGSYLLKAVHKRNGNTVAESLGSVALSYPLEYLRSSSDTSA"
     gene            complement(2544..4637)
                     /locus_tag="VFV99_08185"
     CDS             complement(2544..4637)
                     /locus_tag="VFV99_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5059322.1"
                     /translation="MRFLGPIAGSMWAYVALGAAVLAVGAYIIKMRRRRFEVPFVQLW
                     KRVLEQKDANALWKQLKRLISLLLMLLILGLILFAALDPTLGVADRKARSVVILVDSS
                     GSMKTLDGDPAGTKSRMEIAKQKADDLIDSMGGGDVAIIMKVDGQSTPMTRFTNDKAM
                     LHKALHGVGRSTQSKEQNLTLDGITASDTPADLSRALGAAADALRDRKNPLIVLISDG
                     AFPEAQLGLVTWEKKPAGSGSAANPAAEKNLASVDLSGIDVRYIPVGRRSDNVGIVAF
                     NVRRYIANKAAYEVFIEVQNFGTEPAKRVLTLQNGDTPVDTRDITLAPGQRMRQIYEK
                     LPASEDYKLRAQLKPAIGPDKQIVKGGSDAFALDDIAYALLPARKKQKVLMVTADNLF
                     LEAALLVYDNADPLKVTPDEYNAKPQIADGMDVVIFDDFTPEVVPSPPTSLLYFHPTG
                     ASSPFKVAGELPNPRITEIDEDHPTMRWITMSDVYTDKSNTFAVDSRKGESTIAYSVR
                     DSIIAAKRENRRKILAVGFSLPAAGRDSATDMPLKVAFPVLIVNALDWFAGDTADLLT
                     TYATGRRERIPLDGVVGATEAVVKGPDGRETRTPVIDGLATFYGSQVGYYDVAAKDAS
                     GHTMAQIALAANLASVDESDIAPSVELTLGGKKLQAPEAFEITRSQKLWWYFVLLAMA
                     LIVTEWITYHRRITV"
     gene            complement(4634..5572)
                     /locus_tag="VFV99_08190"
     CDS             complement(4634..5572)
                     /locus_tag="VFV99_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5059323.1"
                     /translation="MVAVPAQAPAAAKVNRAEDRSGLFDERFLKMLEHLHMVSRKVVS
                     GNLRAERRTRKVGSGIEFADHRTYARGDDFRYIDWNLYGRLDKLLLRLFEEEEDLAIY
                     ILIDVSDSMAIGSPLPKLHYAMQIGAALTYVGLANLDRVAIIPFADRLIDRMPPARGK
                     NRIFRVFDFLRKCDIGGQTNLAECMKDFVTQNKRRGLAVVISDFYDPVGFEQGINTLR
                     YHKFEPFMLQAYDLREASPALHGDLTLVDCETGDTREVTVSKSLLEAYEKEHEKYCKE
                     LEAYCTKYAMPFFRTHTSIPFDELVLKIFRSGGFLR"
     gene            complement(5572..6570)
                     /locus_tag="VFV99_08195"
     CDS             complement(5572..6570)
                     /locus_tag="VFV99_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5059324.1"
                     /translation="MTQAAALDVESTVNTFTADLRRLREEIGTMIVGQEEIVEGVLMC
                     LLGGGHALLEGVPGLGKTMLVRTLAETIHATFSRIQFTPDLMPADIVGTNVITEESGV
                     KRFEFSKGPIFANIVLADEINRATPKTQSALLEAMSEGSVTVAKQTYNLEKPFFVLAT
                     QNPLEMEGTYPLPEAQLDRFFFKLVVDYPTRENLHTILDRTTTDHKPKPQVVIDKSRI
                     MEMRELVLKVPLAHQIRDYAVRVVLATHPENPEATALSKQFMRYGASPRGLQAIILAA
                     KIRALLEGRYAVSIDDIRHVAAPALRHRLILNFEGEAEGVQADTIIAEILKATKEA"
     gene            complement(6567..8273)
                     /locus_tag="VFV99_08200"
     CDS             complement(6567..8273)
                     /locus_tag="VFV99_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059325.1"
                     /translation="MIRAALFAALLGAAACVSEPEEPACVFDPTAAQLPCLPPGSTVM
                     LGDTRVEVIEWTPDHIKIKIPKDAPEGLTKLHVLLGDREIGSVGFAVASLKDKDLHDP
                     DAVELDPDEKQYVEAILNELKNVAQRDQVKELDEFANKLKDLLEKAENGEITKQQLLE
                     ELSKAEQALNQNAEPNEQEVNKQLGEVGKELAKEQMTKELGEALQKNDLQKAKEEFEK
                     LAEKLDKKELSDKQKEELAKQLEKVAKQMEKQEQEQQKKQDDAQKKVEDQIQKMEKEI
                     RRLEKNKDQKSQQQKQEMERRLEEKKRELDKLKKDEEQKQQSEQRRAVKRLQKDLEKA
                     AENLQKPQKDPNQQNQQDQEQQDQQASQKLKDAARETGRVDQDQRKQATQKKMSSQMD
                     DLREAMRRAKQRGNKGPNDPFNKNGKNQDFIARARGQKGSGQAWKPGQGQGQGQGQGQ
                     GQGQGQGQNGGQGSGSGQQSNTWGTGHDDNLAGDPTGKNGNTKDQDLEGKQGQEGGST
                     RETILAAAQKGFASKRYQKVYQDYQRIVEEVMRTEKLPSSYKYYVKRYFAKIHPNMAE
                     GQ"
     gene            complement(8270..8893)
                     /locus_tag="VFV99_08205"
     CDS             complement(8270..8893)
                     /locus_tag="VFV99_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059326.1"
                     /translation="MAQLTDTSAIQRLVRGARVRIRLQWALEGATTATVFAATLALVS
                     IFCMRTQWIRPNTGIALLVCAGLVIVAGAVICAARKLDDELVARIIDRASNLSDRLST
                     AIAFERVLRWGAASDPDLTHEMMHAAIRDGVRAAPRANLRAATPFRAPKDWRAALGFL
                     VISALAAGIAIPHTDRSPHIYGIDKDKARPGTEVTIQGENLVPGARK"
     gene            complement(9037..10014)
                     /locus_tag="VFV99_08210"
     CDS             complement(9037..10014)
                     /locus_tag="VFV99_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX5059327.1"
                     /translation="MRVVAVALLFITCLAAGCYSPGYRDCEITCASGACPSGFSCDQG
                     VCRIDGFSGPCGQQMSDAPNQFLDAMIDGNPMLDSDGDGIKDNVDNCRLKSNPNQENE
                     DGDLFGDVCDPCPPSQNNTDGDGDGVGDLCDPEPTQTGNVITLFDGFNYAQPPANVVL
                     FPSASWTFPLGQVRLANLGGNQRAAYVFPQQGNGQNIVDAVYTSYSISQISTGIPAGV
                     GVVQRFDASQQNGVACLMGRGVTGIPSIRVVATNDEPSARESAAPFQSGQVVEMVFFR
                     AGQQYDCRDLVSPSAVTAPQQFIVPTASVGLYSQGTQADFNWIMVVSRQ"
     gene            10056..11807
                     /locus_tag="VFV99_08215"
     CDS             10056..11807
                     /locus_tag="VFV99_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059328.1"
                     /translation="MPGCPQCGRHWPDSYSRCPDDGTALIPALGVPTGSGAAPELAYP
                     SSDEDLPNGSLAGEYRIEKKIGEGGMGAVYGAKHPLIGKRAAIKVIRRELSSSREAVD
                     RFVLEAQSVNQIGHPNIVDVFGFGQLPDGRSFFVMEWLQGESLRERLQRPLQFPEAIE
                     FLEQIAKALHAAHEAGVVHRDLKPDNVFLESLKGEKPQVKLLDFGLAKLTGSDSQRSD
                     RTRTGVVMGTPLYLSPEQAKGAKIDASTDVYSLGAMAYEMLAGVVPFKADSAVEIMAM
                     HISQRAIPLMQIAPWVPLDINNLVHTMLEKDPRQRPTAAQVRERLAACRSTAGAVTIG
                     VAMAPSSQSPSSQPHWTPQVPQSAPPGVMQQPGSQPPMMAHAAMAQSGMAQPSGSKKM
                     ITILALLALIGAGLGVVLYIAFGKKQTTPANEQPSKVEVPSTEPAKTEPAKTEPATDP
                     ATTEPAKPEPAKTEPAADPTTAEPAKTEPAKTEPAKTEPALKTIAKPKGKVNVTFEGP
                     AKAMIVVDGKPYGRETDGRVTIELPAGDHTVRVQAKGYKAADTKVRVDAGGSRDVKLT
                     LEKKKTSTNSVHDPFAD"
     gene            11820..12698
                     /locus_tag="VFV99_08220"
     CDS             11820..12698
                     /locus_tag="VFV99_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059329.1"
                     /translation="MRRTILGAVLVLAVATTAHADDKKKAKALYDEGLKHYNLAEYPE
                     AIKSWKEAYLLSKKPLLLFNIGQAFRLSGDCKQAMTFYDSYQREEPSPKNQDELDEAL
                     KLCKDAKPADKPADKPADKPVITTKPADKPAVSVTPPPTDKPADKPVATPPPADKPVV
                     TPTVGQAEPSAPEEPEDTGPATTGGGLRKIGIGVGIAGLVIGGAGVYFALDSASASKD
                     LDGYMGPWGQEQVDLEARGKRSATLGWALGIGGAAAVVAGGVMFAIGGPKAVETTSVS
                     VIPTNGGAAFSWAQRF"
     gene            12847..13482
                     /locus_tag="VFV99_08225"
     CDS             12847..13482
                     /locus_tag="VFV99_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059330.1"
                     /translation="MRIALAFVVASVASFAATGCIMPKNGPAAGNAVPTGEPLAVVDD
                     VKVWTTTYKEKVAEAEYHDENGNKIGSAAVYEDRTQVHTQPIWYPVQGKAQLADEDFF
                     RIAGDKSSLDETLSMRQSGRKWRKRGMITLGGGIVGLIGSYFIPNAPARLAVTLGSTL
                     AFAGGYYMMFWGAHQLNPETHAVDRSVADRAAIQYNQNLGHVGGVSLNKNF"
     gene            complement(13479..>14661)
                     /locus_tag="VFV99_08230"
     CDS             complement(13479..>14661)
                     /locus_tag="VFV99_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059331.1"
                     /translation="ALAAALLRARTDSREARVRFMVTLWAVCGAAFFFVVQTKFNHYI
                     LPILPALGLLIAFFLDDIVARRDRLHPIYALLGVGILLLITRDLMWEPDRWIEMFVFR
                     YDRPWPSIEPYVVDPSDGFLALGIAGSIALLLATLPWRKVGVAALCAVGLAVCIWCLQ
                     SYMPEAGTHWGMRDAVRTYYKQRTIYGEKIVYFSARNVYNDWRASGDVRHSFDTVIPD
                     TLYLGQPMMIRVQLNKPDKEDQVELEMGMLGAVTKIGDHSIEVTIPASERAKIDALVA
                     RGKSEKAKQVRPPVHVVDADRLLAWQLYWRGENFWTADEIFGPVPEMRTGFNKPDNGD
                     FLKYLGDRSKAPLGRRYFVVTEAGRASSVRGVVPTARAKESFEIINTTSNKFSLVGFY
                     L"
BASE COUNT         2416 a         4680 c         4778 g         2787 t
ORIGIN      
        1 cgcggacgtg tccgaagaag agcgtaggta ttcgaggggg taggacaggg cgaccgagcc
       61 gagcgattcg gcgaccgtat tgccgttgcg cttgtgcacg gctttgagca gatagctgcc
      121 gtacttctgg atcttgaagt ccgccgtgta gcggcctgcc gcggtctgcg ccatcggctg
      181 cgtcgacgtg accttgttgt tcgctggatc gacgacctgc agctcggtgt cgagctcgtt
      241 gacgaacttg tcgccggagt cgatcgcgtc gacggtgatc tgcgcgcggc cgtcggcgac
      301 cttcgcgtag aggtcgtagc tgtcgtagac cttgcggcgc atcgacgagc ggatgacctg
      361 cgcccagaac tttggatagc cgccccagcg gatccagtcg acgctccagc ggttcttgac
      421 gtcgctcgtc caggcgacgc tcgtgccgct gccgtagcgc cagcgcgcga ggatcggctc
      481 gccgagatcc gagatcagga tcgtctccga ggtcggcttc ggcttcgtgg tgacgtagcc
      541 gtggagtggt ggcgcgttct cgacgctcgt gccttcgatc gcttcgacct tcttcgcgac
      601 gcgaacgtgg atgaggtcct cgacgagctg gctcttctgc gcctcttgcg tctccttcat
      661 gaagatcttc ggcagcgagc cgatgtcttc gaccatgtag aggcggcctt cgccggtgtc
      721 ggcgatcatc gcgagcatat tgcgatcggc gccttgcacg ccgacggcgg acaccgtgat
      781 gcgcgacgcg cgcatgtcgc tgacgagctc ggcgaggccg tccgtcggcg cttcaccgtc
      841 ggagagcaga atgacgtgct tcaccttcgc gttgatgccc tgcaggatct cgaacgcttc
      901 cttgaggccg ggaaagatgt tcgtgccgcc gcccgactgc aggcgcgcga tgtcgttcga
      961 gatgcgcatc ttgctgctcg cgcgcgtcgg gcgcacgtag acctgtgcct cgctgtcgaa
     1021 cgcgacaacc gcgatgtagt cctgtggcga cagcacctcg gccgtgacgc gcgctgattc
     1081 cttcgcagct tcgagcttgg ggccctgcat cgagcccgag cggtcgatca cgaggacgag
     1141 cgcgatgtcg ggctgctcct tgatcttctc ggagtcgaag cggacgggca tcatgttctc
     1201 gatcgtcgtg cgctcgtagc cgcccgagcc gaacgagtct tcgccgcccg ccatgatcag
     1261 gccgccgccc atgcctttga cgtaggtgtc gagcgcggcc atctggccgg cgcccatgag
     1321 gtgagccggc acgtcgctga cgagcacgag gtcgtacttc tcgagctcct tcgggttcga
     1381 cgggatgccc gtcgggccgc gcaccgaaac atcgatgttc tcgtgctcga gcgccttctg
     1441 cagataactc gcgctgcccg gctcgcgcat cacgccaccc tcgacgtaca gcacgttcgg
     1501 acggccgcgc accggcgcgg tcatcactgc gacgttgttc ttcttctcgg tgtccttcgc
     1561 gacgttcgac aggcgcacct tgtacgtcgt cgcacctgcg cgcttcgcgt cggacttgaa
     1621 cttgacgagg ttcttgccct cgcgcagctc gacggtcttc gacggctcga ggccgttcgg
     1681 gaactcgtcc tgctgcagcg ccagcgtgac ggtctgcggc tcggtcgacc acagctcggc
     1741 ggtgacctcg aacggctggc cgactttgat gtcgtcgggc agcgtgaggc cggcgacgcg
     1801 cacttcggcg gtcttgtcgg cgtcgaacgt gcgccacgac accttcactt tgagctcttt
     1861 ggcgcgatac gcctcgatcg ccatgtcgcc ttcggtctga ttgccgtcgg agatgatgac
     1921 catgcgatcg acgtacccgt cggggtacat gccgtacgcg agctgcatcg cggactgcgt
     1981 atcggtaccc gcgccgccat cggcgtgacg cttgatcgcg ctcgacagcg gcttgtcgtc
     2041 ctccgcccgc gcgaccagcg gtttctccgc gaacgtgatc agctgcaggc tgccgttgcc
     2101 ttcggctttc tcgagctcgt cgacgtactt cttcgcggca tcgagctgct tgtccgagat
     2161 cgagtcggag acatcgacga gcacgatcgt cgcgaccttg ttcgtctcgg taatccatga
     2221 cggtcgcgcc gccgcaaccg cgagagccgc gatcacgagc gaacgaagcg tcgcctgcac
     2281 cacttgctgg aggaccgaga ggtcggtcag cgacagcacg cggagtagga acaagaatgg
     2341 aacgatcgcg atcagcagca gcttgcccgg atcgagcagc gtgatcgggc gcgagccgat
     2401 gtcgacgggc agcgtggcgc gaccaccgat ccaacggtcg acgagcatgt agatgccgat
     2461 cgacgcggcg agcgcgacga ggatgcgcac gaacaccgcg gggcgcgcga gctcgcgaac
     2521 gatccgcttc gccgacagcg ccattagacg gtgatcctcc ggtgataggt gatccactcg
     2581 gtaacgatca gcgccatggc gaggagaacg aagtaccacc acagcttctg gctgcgcgtg
     2641 atctcgaacg cttccggtgc ttgcagcttc ttaccgccga gcgtgagctc gacggagggc
     2701 gcgatatcgc tctcgtcgac ggacgcgaga ttcgcagcaa gtgcgatctg cgccatcgtg
     2761 tggccgctgg catccttcgc ggcgacgtcg tagtagccga cctgcgagcc gtagaacgtg
     2821 gcgaggccgt cgatcaccgg cgtgcgcgtc tcgcggccat ctgggccttt gacgaccgcc
     2881 tcggtcgcgc caacgacacc gtcgagcggg atgcgttcgc ggcggccggt cgcgtaggtc
     2941 gtcagcagat ccgccgtgtc accggcgaac cagtcgagcg cgttgacgat caacaccggg
     3001 aacgccacct tcagcggcat gtcggtcgcg ctgtcgcgtc cggctgcggg tagcgagaag
     3061 ccgaccgcga ggatcttgcg gcggttctcg cgctttgccg cgatgatgga gtcacgcacc
     3121 gagtaggcga tcgtcgactc gccctttctt gagtcgaccg cgaacgtgtt gctcttgtcc
     3181 gtatagacgt cggacatcgt gatccaccgc atcgtcgggt ggtcttcgtc gatctcggtg
     3241 atgcgggggt tcggcagctc gccggcgacc ttgaacggtg agctcgcgcc ggtcgggtgg
     3301 aagtagagca gggacgtcgg tggggatgga acaacctcgg gcgtgaagtc gtcgaagatc
     3361 acgacatcca tgccgtccgc gatctgtggc ttcgcgttgt attcgtccgg cgtcaccttg
     3421 agtggatccg cgttgtcgta gacgaggagt gcggcctcga ggaacaggtt gtccgcggtg
     3481 accatcagca ccttctgctt cttgcgcgcg ggcaagaggg cgtacgcgat gtcgtcgagc
     3541 gcgaatgcat cgctgccgcc cttcacgatc tgcttgtcgg gaccgatcgc gggtttgagc
     3601 tgggcgcgca gcttgtagtc ttcgctcgcc ggcaacttct cgtagatctg gcgcatgcgc
     3661 tggccgggtg ccagcgtgat gtcgcgcgtg tcgacgggag tgtcgccgtt ctgcagcgtc
     3721 agtacgcgct tcgccggctc cgtaccgaag ttctggacct cgatgaacac ctcgtacgcg
     3781 gccttgttcg cgatgtagcg gcgcacgttg aacgcgacga tgccgacgtt atcgctgcgg
     3841 cggccgaccg ggatgtagcg cacgtcgatg ccggacagat cgaccgaggc caggttcttc
     3901 tctgcggccg ggttcgccgc gctgccgcta cccgccggct tcttctccca cgtgacgagg
     3961 ccgagctgcg cttcggggaa cgcgccgtcg gagatcagca cgatcagcgg gttcttgcga
     4021 tcgcgtagcg cgtccgcggc ggcaccgagc gcgcgcgaca gatcggccgg cgtgtcgctc
     4081 gcagtgatgc cgtcgagcgt caggttctgt tctttagact gagtcgagcg gcccacgcca
     4141 tgtagcgcct tgtgcagcat cgccttgtcg ttcgtgaacc ttgtcatcgg cgtcgactgg
     4201 ccatcgacct tcatgatgat tgcgacgtcg ccgccaccca tcgaatcgat caggtcgtcg
     4261 gccttctgct tggcaatctc catgcggctc ttcgtgcctg ccggatcgcc gtcgagcgtc
     4321 ttcatcgagc ccgagctgtc gacgaggatc acgacgctgc gtgccttgcg atcggcgacg
     4381 ccgagcgtcg gatcgagagc agcaaacagg atgagcccga ggatcagcag catcaagagc
     4441 agcgaaatga gtcgcttgag ctgcttccac agcgcgttgg cgtccttctg ctcgaggacg
     4501 cgcttccaca gctggacgaa cggcacctcg aagcgacgcc gccgcatctt gatgatgtac
     4561 gcgccgaccg cgagcaccgc cgcgccgagc gcgacgtagg cccacatgct gccggcgatc
     4621 ggtccgagga acctcacctc aagaaccctc ccgagcggaa gatcttgagg acgagctcgt
     4681 cgaacggaat cgacgtgtgc gtgcggaaga acggcatcgc gtacttcgtg cagtacgcct
     4741 cgagctcctt gcagtacttc tcgtgctctt tctcgtaggc ctcgagcagc gacttcgaca
     4801 ccgtcacttc acgggtgtcg ccggtctcgc aatcgacgag cgtgagatcg ccgtggaggg
     4861 caggcgaggc ctcgcgcagg tcgtaggcct gcagcatgaa cggctcgaac ttgtgatagc
     4921 ggagcgtgtt gatgccctgc tcgaagccga ccggatcgta gaagtccgag atcacgaccg
     4981 cgagcccgcg acgcttgttc tgcgtcacga agtccttcat gcactcggcg aggtttgtct
     5041 gcccgccgat gtcgcacttc cgcaagaagt cgaacacgcg gaagatgcgg ttcttgccac
     5101 gcgcaggcgg catgcgatcg atcaagcgat cggcgaacgg gatgatcgcg acgcgatcga
     5161 ggttcgcgag cccgacatac gtcagcgcgg cgccgatctg catcgcgtag tgcagcttcg
     5221 gtagcggcga accgatcgcc atcgagtccg aaacgtcgat caggatgtag atcgcgagat
     5281 cctcttcctc ctcgaacagt cgcagcagca gcttgtcgag gcggccgtag aggttccagt
     5341 cgatgtagcg gaagtcgtcg ccacgcgcgt aggtgcggtg atcggcgaac tcgatgccgg
     5401 agccgacttt acgcgtgcgg cgctcggcgc ggaggtttcc ggacacgacc ttgcgcgaca
     5461 ccatgtgaag gtgctcgagc atcttgagga atcgctcgtc gaacagaccc gagcggtctt
     5521 ccgcgcggtt cactttcgcc gcggccggcg cctgtgcggg aacagcgacc actacgcctc
     5581 tttggtcgcc ttcaggattt ccgcgatgat ggtgtcggcc tgcacgcctt cggcttcacc
     5641 ttcgaagttg aggatcaagc ggtggcgcag cgccggcgcc gcgacgtggc ggatgtcgtc
     5701 gatcgatacc gcgtagcggc cttcgagcag cgcacggatc ttcgcggcga ggatgatcgc
     5761 ctgcaaaccg cgcggcgacg cgccgtagcg catgaactgc ttcgacagcg ccgtcgcttc
     5821 cgggttctcc gggtgggttg cgagcacgac gcgcacggcg tagtcgcgga tctggtgcgc
     5881 gagcggcacc ttgagcacga gctcgcgcat ctccatgata cggctcttgt cgatcacgac
     5941 ctgcggcttc ggtttgtgat cggtggtcgt gcgatcgagg atcgtgtgga ggttctcgcg
     6001 cgtcgggtaa tcgacgacga gcttgaagaa gaaacgatcg agctgcgctt cgggcagcgg
     6061 atacgtgcct tccatctcga gcgggttctg cgtcgcgagc acgaagaacg gcttctcgag
     6121 gttgtaggtc tgcttcgcga ccgtgaccga gccttcgctc atcgcctcga gcagcgcgga
     6181 ctgcgtcttt ggcgtcgcgc ggttgatctc gtcggcgagc acgatgttcg cgaagatcgg
     6241 acccttcgag aactcgaagc gcttcacgcc gctctcttcg gtgatgacgt tcgtaccgac
     6301 gatgtcagcc ggcatgaggt cgggcgtgaa ctggatgcgc gagaacgtcg cgtgaatcgt
     6361 ctcggcgagc gtgcgcacga gcatcgtctt gccgaggccg ggcacgcctt cgagcagcgc
     6421 gtgaccaccg ccgagcagac acatcagcac gccttcgacg atctcttcct ggccgacgat
     6481 catcgtgccg atttcttcgc gcagtcggcg gaggtctgcg gtgaacgtgt tgacggtgga
     6541 ctcgacgtcc agtgctgctg cttgggtcat tggccctctg ccatgttcgg gtgaatcttc
     6601 gcgaagtaac gtttcacgta gtacttgtag ctcgacggta gcttctcggt acgcatcacc
     6661 tcttcgacga tgcgctgata atcctgatag accttctgat agcgcttgga ggcgaagccc
     6721 ttctgcgcag cagcgaggat cgtctcgcgc gtcgagccgc cttcctggcc ctgcttgccc
     6781 tcgaggtcct gatccttcgt gttgccgttc ttgccggtcg gatcaccggc gaggttgtcg
     6841 tcgtggcccg tgccccaggt gttgctctgc tggcccgagc cgctgccctg accgccgttc
     6901 tgaccttggc cctgaccttg gccttgtccc tgaccttggc cctgtccctg accttggccg
     6961 ggcttccacg cttggcccga gcctttctgg ccgcgcgcac gcgcgatgaa gtcctggttc
     7021 ttgccgttct tgttgaacgg atcgttcggg cccttgttgc cgcgctgctt cgcgcgccgc
     7081 attgcttcgc gcagatcatc catctgcgac gacatcttct tctgcgtggc ctgcttgcgc
     7141 tggtcctggt cgacgcgacc ggtctcgcgc gcggcgtcct ttagcttctg cgacgcctgc
     7201 tggtcctgct gttcctgatc ctgctggttc tgctggttcg gatctttctg cggcttctgg
     7261 aggttctccg ccgccttctc gagatccttc tgcagacgct tcacggcgcg acgctgctcc
     7321 gactgctgct tctgctcttc gtctttcttc aatttatcga gctcgcgctt cttctcttcg
     7381 agacgacgct ccatctcctg cttctgttgc tggctcttct ggtctttgtt cttctccagc
     7441 cgacggatct ccttctccat cttctggatc tggtcttcga ccttcttctg cgcgtcgtcc
     7501 tgcttcttct gctgctcctg ctcctgcttc tccatctgct tggcgacctt ctcgagctgc
     7561 ttcgccaact cttccttctg cttgtccgag agctccttct tgtcgagctt ctcggcgagc
     7621 ttctcgaact cttccttcgc cttctgcaga tcattcttct gcagcgcttc gccgagctcc
     7681 ttcgtcatct gctctttggc gagctctttg ccgacctcgc caagctgctt gttgacctct
     7741 tgctcgttcg gctcggcgtt ctggttgagc gcttgctcgg ccttgctgag ctcttcgagc
     7801 agttgctgct tcgtgatctc gccgttctcg gccttctcga gcaagtcttt cagcttgttc
     7861 gcgaactcgt cgagctcctt gacctgatcg cgctgcgcga cgttcttcag ctcgttcaag
     7921 atcgcttcga cgtactgctt ctcgtcggga tcgagctcga cggcgtccgg atcgtgcagg
     7981 tccttgtcct tgaggctcgc gaccgcaaag ccgaccgagc cgatctcgcg atcgccgagg
     8041 agcacgtgca gcttcgtcag gccctcgggg gcgtccttcg gaatcttgat cttgatgtga
     8101 tcgggcgtcc actcgatcac ctcgacgcgc gtgtcgccga gcatgacggt cgagccaggt
     8161 ggcaagcacg gcagctgcgc ggccgtagga tcgaagacgc acgcgggttc ctcgggctcg
     8221 ctcacgcacg ctgcagcgcc gagcagtgca gcaaacaggg ccgcgcggat cacttgcgcg
     8281 ctcccggtac gaggttttca ccttgaatcg tcacttcggt tcccgggcgg gccttgtcct
     8341 tgtcgatgcc gtagatgtgc ggcgaccgat cggtgtgtgg gatcgcgatg ccagccgcga
     8401 gcgccgagat cacgaggaag ccgagtgcag cgcgccagtc cttcggcgct ctgaacggag
     8461 tcgcggcgcg taggttcgcg cgcggtgccg cacgaacgcc atcgcgaatc gccgcgtgca
     8521 tcatctcgtg tgtcagatcg gggtcggatg cagctcccca ccgcagcacg cgctcgaatg
     8581 cgatcgcggt cgatagtcga tcagacagat tcgacgcgcg atcgatgatg cgcgcgacca
     8641 gctcgtcatc gagcttgcgg gcagcgcaaa tcaccgcccc cgccacgatc acgaggcccg
     8701 cgcagaccag taatgcgatg ccggtattcg gccggatcca ctgcgttcgc atgcagaaga
     8761 tcgagaccag cgcgagcgtc gcagcgaaca ccgtggcggt cgttgcgccc tcgagggccc
     8821 actgcaggcg gatgcgaacg cgggccccac ggacgagccg ctggatcgct gacgtatcag
     8881 ttagttgcgc catggctcct cgcgaggtcc gagagctcca acggttgagg atagcaccaa
     8941 cattccacgc gccagcctga ctgacgatct aagcgttcga cacccaaggc gtcgtacggt
     9001 tgcccgcgga tccagcctac atgtcggctc gggcaattac tggcgagaga cgaccatgat
     9061 ccagttgaag tcggcctgcg tgccctgcga gtacaggccg acgctcgcgg tcggtacgat
     9121 gaactgctgg ggggcagtca ccgccgatgg agagaccaga tcgcgacagt catactgctg
     9181 acccgcgcga aagaagacca tttcgaccac ctgaccactc tggaacggcg ctgcgctctc
     9241 tcttgcactg ggttcatcat tggtcgcgac aacgcgaatg ctagggatgc cggtgacgcc
     9301 gcgtcccatc aggcacgcga cgccgttctg ttgcgacgca tcgaagcgct gcacgacacc
     9361 gacgcccgcc gggatgccgg ttgatatctg cgagatgctg tagctcgtgt acacggcgtc
     9421 gacgatgttc tgaccgttgc cttgctgcgg aaacacgtag gccgcgcgct ggttgccgcc
     9481 gaggttcgcg agccgcactt gtccgagcgg aaatgtccac gacgcgctcg gaaacaacac
     9541 gacgttcgcc ggcggctgtg cgtagttgaa gccgtcgaac agcgtaatga cgttaccggt
     9601 ctgagtcggc tccgggtcgc acaggtcgcc gacgccgtcg ccatcgccgt cggtgttgtt
     9661 ctggctcggc gggcacggat cgcatacgtc gccgaatagg tcgccgtctt cgttctcttg
     9721 gttcgggttc gatttgaggc ggcagttgtc gacgttgtcc ttgatgccgt cgccgtcgct
     9781 gtcgagcatc gggttgccgt cgatcatcgc gtccaagaac tgattcggtg cgtcgctcat
     9841 ctgctggccg cacggcccgg agaagccgtc gatgcgacac acgccttggt cgcacgagaa
     9901 gccggacggg cacgcgcccg acgcgcacgt gatctcgcaa tcgcgatagc cgggcgagta
     9961 acacccagct gccaggcaag taatgaaaag caaagcgaca gcgacaacac gcacgccgtg
    10021 atcttacacg agtgaccagc tacaatgtcc gtccgatgcc ggggtgtcct cagtgtggtc
    10081 gtcattggcc tgacagctat tcgcgctgcc ccgacgatgg cactgcgttg atcccggcgc
    10141 tgggcgtacc tactggtagt ggcgctgcgc ccgagctcgc gtatccatcc tccgatgaag
    10201 atctgccgaa cggcagtctc gccggcgagt accgcatcga gaagaagatc ggcgaaggcg
    10261 ggatgggcgc ggtgtacggc gcgaagcatc cgctgatcgg caagcgcgcc gcgatcaagg
    10321 tcatccgtcg cgagctgtcg agcagtcgcg aggccgtcga tcgcttcgtg ctcgaagcgc
    10381 agtcggtgaa ccagatcggc cacccgaaca tcgtcgacgt gttcggcttc ggccagcttc
    10441 ccgatggtcg cagcttcttc gtcatggagt ggctgcaggg cgaatcgctg cgcgaacggc
    10501 tgcagcggcc gctgcagttt cccgaggcaa tcgagttcct cgagcagatc gcgaaggcat
    10561 tgcacgcggc gcacgaagcg ggcgtcgttc accgcgatct gaagcccgac aacgtcttcc
    10621 tcgagagcct caagggcgag aagccccagg taaagctcct cgacttcggt ctcgcgaagt
    10681 tgaccggcag cgactcgcag cgttcggatc gcacgcgcac cggcgtcgtg atgggcacgc
    10741 cgctctactt gtcgccggag caagccaagg gcgcgaagat cgacgcctcc accgacgtct
    10801 actcgctcgg cgcgatggcg tacgagatgc tcgccggcgt cgtgccgttc aaggccgaca
    10861 gcgccgtcga gatcatggcg atgcacatct cgcagcgcgc gattccgctg atgcagatcg
    10921 cgccgtgggt gccgctcgac atcaacaacc tcgtgcacac gatgctcgag aaggatccgc
    10981 gccagcgccc gaccgccgcg caggtgcgcg agcgtcttgc cgcgtgtcga tcgacggccg
    11041 gtgcggtgac gatcggtgtc gcgatggcac cgtcgtcgca gtcgccgtcg tcgcagccgc
    11101 actggacgcc gcaggttccg caatcggcgc cgcccggcgt gatgcagcag ccagggtcgc
    11161 agccgccgat gatggcgcat gccgcgatgg cgcaatcggg catggcgcag ccgagcggca
    11221 gcaagaagat gatcacgatt ctcgcgttgc tcgcgctgat cggcgcgggc ctcggcgtcg
    11281 tgctgtacat cgcgttcggc aagaagcaga cgacgcccgc gaacgagcag ccctcgaagg
    11341 tcgaggtccc gagcacggaa ccggcgaaga ccgagcccgc gaagacagag cccgcgaccg
    11401 atccggcgac gaccgagcct gcgaaaccag aacccgcgaa gaccgagccc gccgcggatc
    11461 cgacgacggc cgaaccggcc aagaccgagc cggcgaagac cgagcccgcc aagaccgagc
    11521 ctgcgctgaa gacgattgcg aagccaaagg gcaaggtcaa cgtgacgttc gagggaccgg
    11581 cgaaggcgat gattgtcgtc gacggcaagc cgtacggtcg cgagaccgac ggacgcgtca
    11641 cgatcgagct gcccgccggc gaccacaccg tgcgcgtcca ggccaagggc tacaaggccg
    11701 ccgatacgaa ggtgcgtgtc gacgcgggtg gctcgcgcga cgtcaagctc acgctcgaga
    11761 agaagaagac gagcaccaac tcggtgcacg atccgtttgc tgactgacta ggagagctca
    11821 tgcgtagaac gatcctcggt gccgtgctcg tgcttgccgt cgcaacgacg gcgcacgccg
    11881 acgacaagaa gaaggcaaag gcgctctacg acgaaggcct caagcactac aacctcgcgg
    11941 agtatccgga agcgatcaag tcgtggaagg aagcctacct gctctcgaag aagccgctgt
    12001 tgctgttcaa catcggccag gcgtttcgcc tctccggtga ctgcaagcag gcgatgacgt
    12061 tctacgacag ctaccagcgc gaagagccga gcccgaagaa tcaagacgag ctcgacgagg
    12121 cgctcaagct ctgcaaggac gcgaagcctg ccgacaagcc agcggacaag cccgccgaca
    12181 aaccggtgat caccacgaag cccgccgaca agcccgcggt cagtgtgacg ccgccgccaa
    12241 cggacaagcc cgccgacaag ccggttgcca caccgccgcc ggcggacaag ccggtcgtca
    12301 cgccgacggt cggtcaggcg gagccgtccg cgcccgagga gcccgaggac acggggccgg
    12361 ccacgactgg cggcggcctg cgtaagatcg gtatcggcgt cggcatcgcc ggcctcgtga
    12421 tcggtggtgc tggcgtgtac ttcgcgctcg acagcgccag cgcgagcaag gatctcgacg
    12481 gctacatggg gccgtgggga caagagcaag tcgatctcga ggcgcgcggc aagcgcagcg
    12541 cgacgctcgg ctgggcgctc ggcatcggcg gtgcagccgc cgtcgttgca ggcggcgtga
    12601 tgttcgcgat cggtggcccg aaggcggtcg agacgacgtc ggtgagcgtg attccgacga
    12661 acggcggcgc ggcgttcagc tgggcgcagc gcttctagcg cgacgcggcg tgagctgtgc
    12721 agcagcgctg cgatcctctc ggcatgtgta gcgacacggc gctgtaacgc gcgggttgtc
    12781 gttttctctg cgactggcgt tcgcggagcg gctcgctatt gcgaaccctc acaaggatcg
    12841 tccgtcatgc gtattgccct cgcattcgtc gtcgcgtctg tcgcttcgtt cgcagccacc
    12901 ggctgcatca tgcccaagaa tggtccggcc gctggcaacg cggtgccgac cggtgagcca
    12961 ctcgccgtcg tcgacgacgt caaggtctgg accacgacgt acaaggagaa ggtcgccgag
    13021 gccgagtatc acgacgagaa cggcaacaag atcggcagcg cggcggtcta cgaggatcgc
    13081 acgcaggtcc acacgcaacc gatctggtat cccgtgcagg gcaaggcgca gctcgccgac
    13141 gaggatttct ttcgcatcgc cggcgacaag tcttcgctcg acgagacgct gtcgatgcgc
    13201 cagagcggtc gcaagtggcg caagcgcggc atgatcacgc tcggtggtgg catcgtcggt
    13261 ttgatcggca gctacttcat tccgaatgcg cctgcgcggc tcgccgtcac gctcggcagc
    13321 acgctcgcat ttgccggcgg ctactacatg atgttctggg gcgcgcacca gctgaacccc
    13381 gagacgcacg cggtcgatcg ctcggtcgct gatcgcgcgg cgattcagta caaccagaat
    13441 ctcggccacg tcggtggcgt gagcctcaac aagaacttct aaaggtagaa gccgacgagc
    13501 gagaacttgt tcgaggtcgt gttgatgatc tcgaacgatt ctttcgcgcg ggcggtcggt
    13561 acgacgccgc gtacgctgct ggcgcggccc gcctcggtca cgacaaagta gcggcggccg
    13621 agcggcgcct tgctgcgatc gccgaggtac ttgaggaagt cgccgttgtc gggcttgttg
    13681 aagccggtgc gcatctcggg aacgggaccg aagatctcgt ccgcggtcca gaagttctcg
    13741 ccgcgccagt agagctgcca cgcgagcaag cggtcggcgt cgacgacgtg taccggcggc
    13801 cgcacctgct tggccttctc gctcttgccg cgtgcgacca gcgcatcgat cttcgcgcgc
    13861 tcgctcgccg gaatcgtgac ctcgatcgag tggtcgccga tcttggtgac cgcgccgagc
    13921 atgcccatct cgagctcgac ttgatcttcc ttgtcgggct tgttgagctg gacgcggatc
    13981 atcatcggct gcccgaggta gagcgtgtcg gggatcaccg tgtcgaacga gtggcgaacg
    14041 tcgccgctcg cgcgccagtc gttgtagacg ttgcgcgccg agaagtagac gatcttctcg
    14101 ccgtagatcg tgcgctgctt gtagtaggtg cgcacggcgt cgcgcatgcc ccagtgcgtg
    14161 cctgcttcgg gcatgtacga ctgcaagcac cagatgcaga ccgcgaggcc cacagcgcat
    14221 agcgccgcga cgccgacctt tcgccagggc agcgtcgcga ggaggagcgc gatcgacccg
    14281 gcgatgccga gcgcgaggaa gccgtcggat ggatcgacga cgtacggctc gatcgacggc
    14341 cacggccggt cgtagcggaa cacgaacatc tcgatccagc gatcgggttc ccacatgaga
    14401 tcgcgcgtga tcagcagcaa gatgccgacg ccgagtagcg cgtagatcgg atgcaggcgg
    14461 tcgcggcggg cgacgatgtc gtcgaggaag aacgcgatca gcaggccgag cgcgggcagg
    14521 atcggcagga tgtagtggtt gaacttggtc tggacgacga agaagaacgc ggcgccgcac
    14581 acggcccaca gcgtgaccat gaagcgcacg cgcgcctcgc ggctgtcggt gcgcgcgcgc
    14641 agcagcgcgg cggcgagcgc g
//
LOCUS       DASUFX010000209         5952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1561713, whole genome shotgun sequence.
ACCESSION   DASUFX010000209 DASUFX010000000
VERSION     DASUFX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561713"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..793
                     /locus_tag="VFV99_08235"
     CDS             116..793
                     /locus_tag="VFV99_08235"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059332.1"
                     /translation="MVAAVMYVGLIGLVLSWAVIAVGSATTAFWFGMPRVAMIACLLG
                     AFVAAAIAIAIIVLLHRGMLSTLSNALARLHIISKQRRERWNTTLSEVDARLRGTDDG
                     DYRRRAIMCVAVSQILQRGLAYFTILAAGYVLSPGQFLALLSAGVVLNWISTIVPMGL
                     GIAEGGNVALFTLIGAPAGLGLALALARRVNQIVFAAIGFFVLAAERLSTHVQGRFTN
                     RLAAKPS"
     gene            796..1176
                     /locus_tag="VFV99_08240"
     CDS             796..1176
                     /locus_tag="VFV99_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX5059333.1"
                     /translation="MLKNAFHKIARRVANMIGTPTAFIVAVLLCIVWAATGPLFGFSD
                     TWQLIINTSTTVVTFLVVFLIQNTQNHDSRALHLKLDELLRALKDARNSLVDLEELPD
                     EDLAKLEAEFRRLRLRTGRRGAHS"
     gene            1439..2953
                     /locus_tag="VFV99_08245"
     CDS             1439..2953
                     /locus_tag="VFV99_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5059334.1"
                     /translation="MNGTILIVDDDEDTAVLLRDSLRKRGFDVDAVTSGQAALEHLRT
                     SPTDVVVTDVQMAGISGIELCQELRQRHPDLLPIVLTGQGGLDTAIAAIRAGAYDFIT
                     KPVKVDALAIAVGRAIEHLSLRREVKRLRSAADRDVPIDGIAGNSPAIRETIELVRRV
                     ADSDATVLVTGESGTGKELVARALHNLSPRREQPFVAVNCAAMPAPLLESELFGHVRG
                     AFTDAKRSRAGLFVQAGAGTIFLDEIGEMPIEMQVKLLRVLQERKVRPVGGDEEVPFE
                     ARVITATNRDLETEVEEKRFREDLFYRINVVAIPVPPLRARAGDILLLAQYFLKRIAG
                     RTSKPVQGISGPAARLLMDYDWPGNVRELENCMERAVALCRLDEITVDDLPAKIQEHQ
                     SSKIVITTESPGELITLDEMERRYVRQVLNAVGGNKTHAARILGIDRRSLYRRLEEPR
                     NDRKEEPKPPGAEGARPPGDGHHAAPPQRPPTAPPHPTAPPPVGANGGQPGELE"
     gene            2975..4066
                     /locus_tag="VFV99_08250"
     CDS             2975..4066
                     /locus_tag="VFV99_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059335.1"
                     /translation="MSERLRLLVVDDDLVDRLAVRRAVEQSGLDTTIDEAGDAEEALQ
                     KVMQRDFDCILLDQDLPGRSGVDIATEIRASGRHTPIVFVTGQQSEDLLQAAVDAGVT
                     DFIPKNDLSPRRLGLRIKFAIRIGAAEAESARAVESARAAARARDEVLAVVSHDLRGP
                     LHAISLATEALRDEIQGPAARYLGAIERASTRAERLISDLLEASALENGAIQLAQAPI
                     DAGAILRQAAAEHELLAKESGGKIVAHIPDEPVVVQADRDRVLQVLGNLIGNSLKHAK
                     GAPIEISVERKDREAVVAVQDHGPGIPPTELPHVFDRYWSGRTKKGGAGLGLAIAKGI
                     VSAHGGAIAVDSKQGEGAKFFFTLPLVRS"
     gene            4208..4330
                     /locus_tag="VFV99_08255"
     CDS             4208..4330
                     /locus_tag="VFV99_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059336.1"
                     /translation="MKEADHPRILELMLGRMLELVVYSVEGVRERYSRGRVVVH"
     gene            4366..>5952
                     /locus_tag="VFV99_08260"
     CDS             4366..>5952
                     /locus_tag="VFV99_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5059337.1"
                     /translation="MRGPVERRLRFDPQRDRARLGVLAEVSTLLASSDDHITILHKLT
                     EMAVPVLGDWCSVHVVGSTGQLERIAIRHADPARLEMADLLARNANHSDPNARGAARV
                     FSGGQSEWVERFNDDLLAEHAREPWARDALRLLGLCSYVAVPLIVRGRVIGVFTLVRS
                     EGGELYDFDDVAFAEELARRVAMYVDNAMLLREVQMREAALRDEATRLETLNRVGQEL
                     AAIHDLDEVVKRVCDAATALTSADIGLYFTYADGETKYALCGATFEEAEEIAKRRDEL
                     TPNNPAFALQRALRMDDVTRAAAATNLGVLSGVERIRSYLAVPVRGRRGDVIGGIALG
                     HSRAATFDVRAEQLVIGLASLTATATDSARLFREAHELIAALEKSNQDLDQFAYVTSH
                     DLRAPLRGIANLSQWVEEDLGARLTDKSREYLHLLRGRVRRLEDLIQGVLDYSRAGRV
                     ADEPTEVNVGQLVREVVELIAPPPEAVVEIDSNLPMLCTTRVPLQQVFMNLLGNAFKY
                     NGKPAAHVQVGAEPIPDGWEF"
BASE COUNT         1006 a         1950 c         1966 g         1030 t
ORIGIN      
        1 acggtggcgc tgttggtgag gtcgagccag gtcactgcga tcgccgcggc caccccgacg
       61 ttgccactgc cgccgctctc gcccgactgg ctgctggagg tgctcgacgc ggcggatcgt
      121 cgctgcggtg atgtacgtag gtttgatcgg cctcgtgctg tcgtgggccg tgatcgcggt
      181 cggcagcgcg acgaccgcgt tttggttcgg catgccgcgc gtcgcgatga tcgcgtgctt
      241 gctcggtgcg ttcgtcgctg cagcgatcgc aatcgcgatc atcgtgctgc tccaccgcgg
      301 catgctgtcg acgctgtcga acgcgctcgc gcgcctgcac atcatctcca agcagcgccg
      361 cgaacgctgg aacacgacgc tttccgaggt cgatgcacgg ctacgcggca ccgacgatgg
      421 cgactaccgt cgtcgcgcga tcatgtgcgt cgccgtctcg cagatactgc agcgtgggct
      481 cgcgtacttc acgatcctgg cggccggcta cgtactgtca cccggtcagt tcctcgcgct
      541 cttgtctgct ggcgtggtgc tcaactggat ctcgacgatc gttccgatgg gcctcggcat
      601 cgccgaaggt ggcaacgttg cgctgttcac gctgattggc gcgcccgcgg gtctcggtct
      661 cgcactcgcg ctggctcgac gtgtgaatca gatcgtattt gccgcgatcg gatttttcgt
      721 acttgccgcc gagcgcctca gcacacacgt gcagggtcgc ttcacgaacc gcctcgcggc
      781 gaagccgagc taacaatgtt gaagaacgcc ttccacaaga tcgctcgccg cgtcgcgaac
      841 atgatcggaa cgccgaccgc gttcatcgtc gcggtgctgc tgtgcatcgt gtgggctgca
      901 accggtccgt tgttcggctt ctcggatacc tggcagctca tcatcaacac ctcgaccacg
      961 gtcgtgacgt tcttggtcgt cttcctgatc cagaacacac agaaccacga ctcccgtgcc
     1021 cttcacctca agctcgacga gctgctccgg gcgttgaagg acgctcgtaa ctcgctcgtc
     1081 gatctcgagg agctcccgga tgaggatctc gccaagctcg aggcggaatt ccgccggttg
     1141 cgccttagga ccggccggcg gggagctcat tcatgagtag ttttcctgcg atctgggcgc
     1201 ccgacgagcg ctggcgcaca gcacatgtag cgggtccgca cggtaaacat gcggatttga
     1261 ggggttgttg ttcggcacgc cgcaagcaag gtgggcgcta gcgctactcg aaaagtttcc
     1321 caacctcgcg ctcagtctgg cgatcatgaa gcaaacggtc gacaggtagc cgccctgaac
     1381 gtgagctgtt tctcaggctg agaagtattc tccggcggca gaaaggcaag agaccgaggt
     1441 gaatggaacc atcctaatcg tcgacgacga tgaagacacc gctgtgctac tgcgggattc
     1501 gctgcgtaag cggggctttg acgtcgatgc ggtcacttcg ggacaggctg cgctggagca
     1561 tctacgcacc tcgccgaccg atgtggttgt caccgacgtt cagatggccg gcatctccgg
     1621 catcgagctc tgccaggagc tccggcaacg ccaccccgac ctgctcccga tcgtgctgac
     1681 cggccaaggt ggtctcgaca ctgccatcgc cgcgattcgc gcgggcgcct acgacttcat
     1741 caccaagccg gtgaaggtcg acgcgctcgc gatcgccgtt ggccgcgcga tcgagcacct
     1801 ctcgctacgc cgcgaggtca agcgcctgcg ttcggccgcc gatcgcgatg tgccgatcga
     1861 cggcattgcc ggcaacagtc cggcgatccg cgaaacgatc gagctcgtgc gccgcgtcgc
     1921 cgacagcgat gcgaccgtgc tggtcaccgg cgagtccggt accggtaagg agctcgtcgc
     1981 tcgcgcgctt cacaacctgt cgccgcgccg cgaacagccg ttcgtcgcgg tgaactgcgc
     2041 cgcgatgcca gcgccgctcc tcgagagcga gctcttcggt cacgtgcggg gcgcatttac
     2101 cgacgcgaag cgttcgcgtg ctggtctgtt cgtgcaggcc ggtgcgggca cgatcttcct
     2161 cgacgaaatc ggcgagatgc cgatcgagat gcaggtcaag ctgctccgcg tgctgcaaga
     2221 gcgcaaggtg cgcccggtcg gcggtgacga ggaagtcccg ttcgaggcgc gcgtgatcac
     2281 ggcgacgaac cgcgatctcg aaaccgaagt cgaagagaag cggttccgcg aagatctctt
     2341 ctaccgcatc aacgtcgttg cgattccggt gcctccgctc cgcgctcgcg ccggcgacat
     2401 cctgctgctc gcgcagtatt tcctgaagcg cattgccggc cgcaccagca agccggtgca
     2461 aggcatctcg ggtccggcag ctcgtctgct gatggactac gactggccgg gcaacgtccg
     2521 cgagctcgag aactgcatgg agcgcgcagt cgcgctgtgc cgcctcgacg agatcacggt
     2581 cgacgacctg ccggcgaaga tccaggagca tcagtcgtcg aagatcgtca tcacgacgga
     2641 atctccgggc gagctgatca cgctcgacga gatggagcgt cgctacgttc gccaggtgct
     2701 caacgcggtc ggcggcaaca agacgcacgc tgcgcgcatc ctcggcatcg atcgtcggtc
     2761 gctgtatcgc cgcctcgaag agccgcgtaa cgatcgcaaa gaagagccga aaccgcctgg
     2821 cgccgaaggt gcccggccac ctggcgacgg ccaccatgcc gccccgccgc aacgcccgcc
     2881 cactgcgccg ccgcacccga cggccccgcc tccggtgggt gccaacggtg gccagcccgg
     2941 cgagctggag tagtaaccta gcgataggtt cgctgtgagc gagcgtctac gcctcctcgt
     3001 tgtcgatgac gacttggtcg accggcttgc ggttcgccgc gcggtcgagc aaagcgggct
     3061 ggatacgacc atcgacgaag cgggcgacgc cgaggaggcg ttgcagaagg tcatgcagcg
     3121 cgacttcgac tgcatcctgc tcgatcagga tctgccgggt cgctccggcg tcgacatcgc
     3181 gaccgagatc cgcgcgtccg gacgccacac cccgatcgtg ttcgtcaccg gtcagcagag
     3241 cgaagatctc ttgcaggcgg ccgtcgacgc cggcgtgacc gacttcattc cgaagaacga
     3301 cttgtcgccg cggcggctcg ggctgcggat caagttcgcg atccggatcg gcgccgccga
     3361 ggcagagtcc gcccgcgccg tcgagagcgc gcgagctgca gcgcgtgcac gcgatgaagt
     3421 cttggcggtc gtctcgcacg acctgcgcgg tccgctccac gcgatcagcc tcgcgaccga
     3481 agcgctcagg gacgagatcc agggcccagc ggcgcgttac ctcggtgcga tcgagcgagc
     3541 gtcgacgcga gccgaacggc tgatcagcga cctgctcgag gcgagcgccc tcgagaacgg
     3601 cgcgatccag ctcgcgcaag cgccgatcga tgccggcgcg atcctgcgtc aggccgccgc
     3661 cgagcacgag ctgctcgcga aggagagcgg cggcaagatc gtcgcccaca ttcccgacga
     3721 gccggtcgtc gtgcaggccg atcgcgatcg cgtgctgcag gtgctcggca atctcatcgg
     3781 caactcgctg aagcacgcga agggcgcgcc gatcgaaatc tcggtcgagc gcaaagaccg
     3841 tgaggcggtg gttgcggtgc aggaccatgg gcctggtatt ccgccgaccg agctgccgca
     3901 cgtgttcgac cgctactggt ctggccgcac gaagaagggc ggcgctggcc tcgggctcgc
     3961 aatcgcgaag ggaatcgtgt ctgcgcacgg cggtgcgatc gcagtcgaca gcaaacaagg
     4021 cgaaggtgcg aagttcttct tcacgctccc gctcgtacgc agctgaacgt cggttaacag
     4081 tgagttagcg cggcgttttc gccgagcgct ttgtagacgg tgagccgggc cgctctctct
     4141 tcacggctta tcgctgtgaa cgcaggctac acgctcgtgg catgcgtcgt gctgaagcac
     4201 agcggcgatg aaggaggccg accacccccg cattctcgag ctcatgctcg ggcgaatgct
     4261 cgagcttgtc gtgtactcgg tggagggcgt gcgcgaacgg tactcgcgcg gacgagtggt
     4321 cgtccactga ctgtgatcac aacacgtttt gacgacgagg acgccatgcg cggccctgtc
     4381 gagcgtcggc tacgtttcga tccgcagcgc gatcgggcgc ggcttggtgt gctcgccgag
     4441 gtcagcacat tgctcgcgtc gtcggacgat cacattacga tcctgcacaa gctcaccgag
     4501 atggcggtcc cggtgctcgg cgactggtgc tcggtccacg tggtcggctc gaccggccag
     4561 ctcgaacgca ttgcgatccg acacgccgac ccggcgcggc tcgaaatggc cgacctgttg
     4621 gcgcgcaacg caaaccacag cgatccgaac gcgcgtggcg cggctcgcgt gttttcgggc
     4681 ggccagagcg aatgggtcga acgcttcaac gacgacctgc tcgccgagca tgcgcgcgag
     4741 ccatgggccc gcgatgcact gcgcctactc gggctgtgct cgtatgtcgc cgtcccactg
     4801 atcgtgcgcg gccgcgtgat cggcgtgttc acgctcgtac gcagcgaggg cggcgagctc
     4861 tacgacttcg atgacgtcgc gtttgcggaa gagctcgcac ggcgcgtcgc gatgtatgtc
     4921 gacaacgcga tgctgctgcg cgaggttcag atgcgcgagg cggcgctgcg cgacgaagcg
     4981 acgcggctcg aaacgctcaa tcgcgtcggc caggagctcg ccgcgatcca cgatctcgac
     5041 gaggtcgtga agcgcgtgtg cgatgcagcg accgcgctga cgagcgcgga catcggcctc
     5101 tacttcacgt acgccgacgg cgaaacgaag tacgcgctgt gcggcgcgac gttcgaggag
     5161 gccgaagaga tcgcgaagcg ccgtgacgag ctgacgccga acaaccccgc attcgcgctg
     5221 cagcgtgcgc tgcggatgga cgacgtgacc cgcgctgccg ccgcgacgaa cctcggcgtg
     5281 ctctccggcg tcgagcggat tcgcagctac ctcgcggttc cggtgcgcgg ccgcagaggt
     5341 gacgtgatcg gtggcatcgc cctgggtcac tcgcgtgcgg ccacgttcga cgtgcgcgct
     5401 gaacagctcg tgatcggcct cgcctcgctg accgcgaccg caaccgatag cgcgcggctg
     5461 tttcgcgaag cgcacgagct gatcgcagcg ctcgagaaga gcaatcagga tctcgatcag
     5521 ttcgcatacg tgacgagtca cgacctgcgg gctccgctgc gcggcatcgc gaatctcagc
     5581 cagtgggtcg aagaggatct cggtgcacgg ctgaccgaca agtcacgcga gtatctgcat
     5641 ctgctgcgtg gccgcgtgcg ccgcctcgag gatctgatcc aaggcgtgct cgactacagc
     5701 cgcgccggac gcgtcgccga tgagccgacg gaggtcaacg tcggtcagct cgtgcgcgag
     5761 gtggtcgagc tgatcgcacc accacccgaa gcggtcgtcg agatcgattc gaatctaccg
     5821 atgctgtgca cgacgcgcgt gccgctccag caggtcttca tgaacttgct cggcaacgcg
     5881 ttcaagtaca acggcaagcc cgcggcccac gtgcaggttg gagccgagcc gattcccgac
     5941 ggctgggagt tc
//
LOCUS       DASUFX010000210         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1421189, whole genome shotgun sequence.
ACCESSION   DASUFX010000210 DASUFX010000000
VERSION     DASUFX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421189"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFV99_08265"
     CDS             <1..601
                     /locus_tag="VFV99_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059338.1"
                     /translation="APKPKPIVTTKAQASKPAVPPPPPLATSGSGSAQVAAQVPTPAQ
                     VPTPAPPPIVAPPPPARGSAAIAQPVKPAPVAPAASPMPAATSPGSLDATPAISKLSV
                     DGSLTTTEVQIALGRTLDTLRTCYRGAAKKANQTPDVSLRVSFEIDEGARANGVRVSG
                     DTFGLGGCVKDAIGGVRTRVAPDVGTVSVSAVVRFKPTR"
     gene            624..1922
                     /locus_tag="VFV99_08270"
     CDS             624..1922
                     /locus_tag="VFV99_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059339.1"
                     /translation="MNARRLSKAMVVWFALGAAAAWAQTPPPEPPPADPQPAPAEPPL
                     PAVEPAPAEPVQAIVEQAPEPEPEPEHEKPKLTRNFAGSVQLDYMAIPTEKTGRQVAL
                     DGATAEVSLKLAMDFTNKISANVKMCVACHGVEVGMAYFDLRVADEMNVRVGRFTPAF
                     GEFPVRHDPANHRTSDKPLPYDMGRMLRINEFNEGVLPAPWVDNGIEVNGTHFFGKNV
                     QADYAAYAISGPRAGSDPMDFDFKQSRSGEAYYIDNNSRPVFGGQLVASVITDNSTFS
                     VGASAMRGTYDPEHKLPFSIYGAHFIARIGDVFLRAEYLTRKYKMSLGEDPTQRFRYG
                     PGANGMFDPFVVKDGGYAELEVPAGDRLTLIAREDGLRRRGNVSLNSMMRSDSALIRH
                     TAGAAVVLRQSLRLKLSYEYYDFSDFEDESVVHVGIAGPF"
     gene            1933..2985
                     /locus_tag="VFV99_08275"
     CDS             1933..2985
                     /locus_tag="VFV99_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059340.1"
                     /translation="MMKYSFILFALVGCDMLKPRVSDETIDAPMAQIDAAIDTPPGGP
                     RFVRPAGTAIPPISDSAELVSQIKIFDGLNDNALATANGVVTRSTGKAAGASVMYWNF
                     GSVPVEGNFVVAAPLYILANDDGTGTLTPRTDHPWLIDTIPGDTRYSPMRRVIYVPVT
                     AAYAGEVIASIDALNEAIELGLVGEPKPAGTWRNIPVVPPGTKLELGTTTAPMESTKV
                     YARGYVVDTLPLAIPQPLSGGRVPSGQEARLLSGVATGTPPVLPIALDAQPVFQYGIP
                     AAPPTTTFNYTPVVTELDVRLATGVAPAAITGDAQLFARSASGSITGYYVDMVNNYVV
                     TTTVSNKQLQFMDGAP"
     gene            2982..3449
                     /locus_tag="VFV99_08280"
     CDS             2982..3449
                     /locus_tag="VFV99_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5059341.1"
                     /translation="MMKYAILMLLVGCSLDYEPDIGRLQADMPDAGFDPDGMPPFVPG
                     LCTDSDPSVSVSFAAQIRPLTSRSPGGCLSCHGASQTSGFSLGSYDSLRRGGANSGTR
                     IVVPGKPCESVIVQKLGLAPPFGSRMPYNGPPYFTSAELTLVRDWIAEGALNN"
     gene            3452..3757
                     /locus_tag="VFV99_08285"
     CDS             3452..3757
                     /locus_tag="VFV99_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059342.1"
                     /translation="MLYRVVLLFALVACGESLPRHSLIANQSDAPPEVREACAFTEQK
                     CTRCHTIGRVLAWDAHTRPQWEPLVTRMRQMASSGITKADGEIVLDCLSHREGLSSR"
BASE COUNT          745 a         1416 c         1307 g          664 t
ORIGIN      
        1 ggcgcccaag ccgaagccga ttgtcacgac gaaggcacag gcgagcaagc ccgcggtgcc
       61 gccgccgccg ccactggcga cgagtggctc gggctcggcc caggttgctg cgcaggtgcc
      121 cacgcctgcg caggtgccca cgcctgcgcc gccgcccatc gtggcgccac ccccgccggc
      181 gcgcgggagc gctgcgatcg cgcaaccggt caagcctgcg ccggtcgcgc cggcagcgtc
      241 gccgatgccc gcggcgacaa gcccaggctc gctcgacgcg acgccagcga tctccaagct
      301 ctcggtcgat ggctcgctga cgaccaccga ggtgcagatc gcgctcggcc gtacactcga
      361 tacgctccgc acttgctatc gcggtgctgc gaagaaagcg aaccaaacgc ctgatgtcag
      421 tctgcgcgtc agcttcgaga tcgacgaggg cgcgcgtgcg aacggcgtac gcgtcagcgg
      481 cgatacgttc gggcttggcg gctgtgtgaa agatgcaatc ggtggcgtac gcacacgcgt
      541 cgcaccggat gttggaaccg tttccgtgag tgcagtggtt cggttcaagc cgacccgcta
      601 gccagcaaca acaagaggtc accgtgaacg cacgacgcct gtcgaaagcg atggtggtgt
      661 ggtttgccct cggcgcggct gccgcgtggg cgcaaacccc gccgcccgag ccaccacccg
      721 ccgatccgca gcccgcacct gcggagccgc cactgccagc cgtcgagccc gcgccggccg
      781 agcctgtgca ggcgatcgtc gagcaagcgc ccgaaccgga gcccgaaccc gagcacgaga
      841 agccgaagct gacgcgtaac ttcgcgggct cggtgcagct cgactacatg gcgattccga
      901 cggagaagac cggtcgtcag gtcgcgctcg acggtgcaac cgcggaggtc tcgctcaagc
      961 tcgcgatgga cttcacgaac aagatctcgg cgaacgtgaa gatgtgcgtc gcgtgccacg
     1021 gcgtcgaagt cggcatggcg tacttcgatc tgcgcgttgc cgacgagatg aacgtgcgcg
     1081 tcggccgctt cacgccggcg ttcggcgaat tcccggtgcg ccacgatccg gcgaaccacc
     1141 gcacgagcga caagccgctg ccctacgaca tgggccgcat gctccgcatc aacgagttca
     1201 acgagggcgt gctgccggcg ccgtgggtcg acaacggcat cgaggtcaac ggcacgcact
     1261 tcttcggcaa gaacgtgcag gccgactacg ccgcgtatgc gatcagcggc ccgcgcgccg
     1321 gctccgatcc gatggacttc gacttcaagc aatcgcgctc cggcgaggcg tactacatcg
     1381 acaacaactc gcgacccgtg ttcggcggtc agctcgttgc cagcgtgatc accgacaact
     1441 ccacattctc cgtcggcgca tcggcaatgc gcggcacgta cgaccccgag cacaagctgc
     1501 cgttctcgat ctacggcgcg cacttcatcg cgcgcatcgg cgacgtgttc ctgcgcgccg
     1561 aatacctgac gcgcaaatac aagatgtcgc tcggcgagga tccgacgcag cgcttccgct
     1621 acgggccggg cgcgaacggc atgttcgatc cgttcgtcgt caaggacggc ggctacgccg
     1681 agctcgaagt acctgcgggc gaccggctca cgctgatcgc gcgcgaggac ggcttacggc
     1741 ggcgcggcaa cgtttcgctc aacagcatga tgcgctccga cagcgccttg attcgccata
     1801 ccgcgggtgc cgcggtcgtg ctgcggcagt cactccgtct caagctcagc tacgagtact
     1861 acgacttctc ggacttcgag gacgaatcgg tggtgcacgt cggcatcgca ggtccgttct
     1921 gaggagatga cgatgatgaa atacagcttc atcctcttcg cgctcgtcgg ttgcgacatg
     1981 ctcaagccgc gtgttagcga cgagaccatc gacgcaccga tggcgcagat cgacgcagcg
     2041 atcgatacgc cgcccggcgg tccgcgcttc gtgcggccgg cagggacggc catcccgccg
     2101 atcagcgaca gcgccgagct ggtcagccag atcaagatct tcgacgggct caatgacaac
     2161 gcactcgcga cggcgaacgg cgtggtcacg cgcagcaccg gcaaggcggc cggcgctagc
     2221 gtcatgtact ggaactttgg ttccgtcccc gtggaaggca acttcgtcgt ggccgcgccg
     2281 ctgtacatcc tcgcgaacga cgacggcacc ggcactctca cgccgcgcac cgaccaccca
     2341 tggctgatcg acacgattcc gggcgacacg cgctacagcc cgatgcgacg cgtgatctac
     2401 gtgccggtca ccgccgcgta cgcaggcgag gtgatcgcgt cgatcgacgc gctgaacgaa
     2461 gcgatcgagc tcggcctcgt cggcgaaccg aaaccggccg gcacgtggcg caacatcccg
     2521 gtcgtgccgc caggcaccaa gctcgagctc ggcaccacca cagcgccgat ggaatcgacg
     2581 aaggtctacg cccgcggcta tgtcgtcgac acgctgccgc tcgcgatccc gcagccactc
     2641 agcggcggcc gtgtgccgag cgggcaggag gcgcgcctgc tgtcgggcgt cgcaaccggt
     2701 acgccaccgg ttctaccgat cgcgctcgac gcgcagcccg tgttccagta cggcattccg
     2761 gccgcgccgc cgacgacaac gttcaactac acacccgtcg tcaccgagct cgacgtgcga
     2821 ctcgcaaccg gcgtcgcgcc ggcggccatc accggcgatg cgcagctgtt cgcgcgtagc
     2881 gcgtcgggat cgatcaccgg ctactacgtc gacatggtca acaactacgt cgtcacgacg
     2941 acggtcagca acaagcagct ccagttcatg gacggtgcac catgatgaag tacgcgatcc
     3001 tgatgcttct cgtcggttgc tcgctcgact acgagcccga catcggtcgt ctgcaagccg
     3061 acatgcccga tgccggcttt gatcccgacg gcatgccgcc gttcgtccca ggcctctgca
     3121 ccgacagcga tccgagcgtc tcggtctcgt tcgcggcgca gatccggccg ctgacatcac
     3181 gctcgccggg cggctgcctc agctgtcacg gtgcgagtca gacctcgggc ttttccctcg
     3241 gtagctatga ctcgctccgc cgcggcggcg ccaactccgg cacgcgcatc gtcgttcccg
     3301 gcaaaccgtg cgagagcgtg atcgtgcaga agctcgggct cgcgccgccg ttcggctcgc
     3361 gcatgcccta caacggcccg ccgtacttca cgtctgctga gctcacgctc gttcgcgact
     3421 ggatcgccga gggggcgctc aacaactagt catgctgtac cgcgtagtcc tgttgttcgc
     3481 gctagtggcg tgcggtgagt cgctgccgcg tcactcgctg atcgcgaacc aaagcgacgc
     3541 gccacctgaa gtgcgtgaag cgtgcgcgtt caccgaacaa aagtgcacgc gttgtcacac
     3601 gatcggtcgc gtgctggcgt gggacgcgca tacgcggccg caatgggagc cgctggttac
     3661 gcggatgcgc caaatggcgt cgagcggcat cacgaaggca gacggagaga tcgtgctcga
     3721 ttgtctctcg caccgcgagg gactctcgtc gcgctgactc ggccctccgt tactgcgctg
     3781 atcgagcagc tgcactcgaa gtgcaaacag gtgtgtgctg caccgactga acgttcggcc
     3841 atgcaaagtt tgcgagtctg cgtcgacttc gcaggctgaa tctcgtccat tcgacaccgc
     3901 gttgcgacgg ggctgtcatc gcacgttgta ccaatgatct ttcgggctgg tgggagatgc
     3961 atccagatga gcctaagtcc tcgacgaaga acaagggatc gattgaagag cgtcgccgca
     4021 tcgcgcgcgg ctctgaacga attgtccaga tagtgaacaa ccgttaccct gactcaatgc
     4081 acccaggctc gggggagcct gacgacgaga acaccgacat aacgcagatc cg
//
LOCUS       DASUFX010000211         8297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1005790, whole genome shotgun sequence.
ACCESSION   DASUFX010000211 DASUFX010000000
VERSION     DASUFX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005790"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1528
                     /locus_tag="VFV99_08290"
     CDS             353..1528
                     /locus_tag="VFV99_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX5059343.1"
                     /translation="MQALGLPAPLHSVSTPVDGGALRVLGSGDQAFQQILGRIRGAQK
                     SVEIRAFLWRDDEAGNLLGEAVLEAADRGAHVVIHKDRIAAVYEYTGGNKQSFFHKRV
                     DPIRGFQAWFLGAVYRAPGSFKQKPNALAQRILEHGNITVEHMRKRFDHSKLYIIDDR
                     YLATGSMGIGDNHRHEWIDVMVEAEGEEHVARLRERMLGHDEFDPSRGLDFLVHSREA
                     HRKKSCPMISHRLALIEAAQQSITVEMAYFTDRRFTAALARAVQRGVEVKVVIAESAD
                     VLGNINRATCDALMRLTGAPQNLSIVLVPRMVHSKVVVIDHRWSDVGSANFTPLSHGV
                     YDEINLYADCEPFARALEAEIDSHCEDGRVADSRLSYRKMYSGLERAIIAYQSRRGG"
     gene            1528..2145
                     /locus_tag="VFV99_08295"
     CDS             1528..2145
                     /locus_tag="VFV99_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5059344.1"
                     /translation="MAATVDPAERAKQERRRQIMNAAKHVFAEAGYHGASIHAIIERA
                     QIARGTFYLYFESKAAVFDSILDQAMADLRARIHRIEVDDKNAPAPQVQLREQVMATL
                     DFVVRDRPLATLLLSAGHTPDAEAAERLDQFFAEVRDLLRRAMESGMEIGLLRKVDPE
                     LAASAMLGMIRGVIEQLIRAETPPAVDVVVSEVLMVALRGVLATG"
     gene            2158..5199
                     /locus_tag="VFV99_08300"
     CDS             2158..5199
                     /locus_tag="VFV99_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059345.1"
                     /translation="MGRVLANRFELGPRVGKGSLSVVHEAIDRESSRRVAIKLAAGLE
                     DLVMVRQRFDREAAALSVISSKHVVELISAGTDEEGLPFLVLERLDGRNLQEVIADRG
                     PMKPERVGRYIAQAAAALELAHGLGIVHRDLKPANLFAHEVGENTHRVVKVLDFGLVV
                     DTGGPTDRLRDAFGGTPLYMAPEQVRGQLTRIGPATDIYAIAQVTLTLLTGETYWTAT
                     SAEDVMREIESSVPSKPSLRWPWLTEPFDQWFLRSTQRVPERRFRSVAAQAAQLVEAL
                     RGIRPPDRKTPAPSFVAASTLAARTPTPSIVRLAGHRTPVIGRQVEYRDVEQLLARGQ
                     VVTLIGPAGIGKTRLSQAICETVGERFIDGTWFVSLVAGQGEKAVLGPIASAVAIEPD
                     ATRSLFEHVVDNLGMRRMLIVLDGAEHVADSARVIDRLVGACPAVTWLVTSRLPLNIA
                     NERKYVVEALDVPRTDHISVDEAETYGGVALFVACAREVAPMFALNERNAEDVVAICR
                     AVAGSLLAIELAAAQVTSSTPAQIRAGIGDGTRSDSAVHQAIMSSYALLAPDQQRMLR
                     QLAILPAGLTFDQVKRKLSHLSDDPMYAVLRLVQTHLATWSSDAPRRLRMLDTVRDLA
                     REQSREAGDDVALWNFAREHAYAIATSSNEMTTEAWLSLVDVEYENLRAVLGRQLSAA
                     PADAMDLAGRLAYYWYLRGDYLEGMQWLEAAIEQSSGEEGEPLARALLGAGRIALLTC
                     RYARAGELLERARSIALAINDLPGEANADQLLGSVARELGEYTRAHSFHVRSLEIWER
                     IGDAREAARARNYLAFAAWIGDYAGMPSDELLEWWDQTNEDELRTLGDPEITVWYLLN
                     RGAILHHRGDPAARDALGRAFAEAIAAKFHEGIAWSLDLIGRVSLERAEFLQARAQLA
                     AALRVHRRLGDRWRCASVLEGLAAVAVASDRPARGAVYLGAADAIREQIGAPVPACER
                     PMLAMTEARGVDMIGQSFDAGRERGRRTPLDQTVELARDVV"
     gene            complement(5196..5648)
                     /locus_tag="VFV99_08305"
     CDS             complement(5196..5648)
                     /locus_tag="VFV99_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059346.1"
                     /translation="MNTKTRQWLVPGLAALVAVTAFVKAWFTAQAVSVGLWGIEVCDH
                     GCQSIRWDNVPGAQDDLYIAGYIAFAAAIVGAATSVVPVFATAVTKVGGRALRVARIS
                     LTIAFLAMLYFITRAIALDGMNGVNISWALPLCPAATIALRQVLPRPS"
     gene            complement(5654..7051)
                     /locus_tag="VFV99_08310"
     CDS             complement(5654..7051)
                     /locus_tag="VFV99_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059347.1"
                     /translation="MVAPAWRSWLPWCVVIAAGWTAIALYSYPGFTSYDSIEQLAQAR
                     GLMPLSDWHLPIMPLTWRVLDKVIAGPLLMVLLQCSLLLAGMQALLTRVLRPWIAVLA
                     TLAVFLWPPVFTLMAIVWKDSLMAGVLVASFAALTSARRGARIAALAGFFYAAMLRHN
                     AIIAIAPMLVLVAPWPARQPLWRRRLAGLAITLVVFGAAQLANRALITERRYPFATSL
                     ATYDIAGMLRYADPLPDATLDRMLAGVPLVAHDHIQRRAELAWSPLSWFPSVDSPRRL
                     MTRPATAAERDAVVSAWWRLIRDQPRAYLLARLRVMRELIGLRMYETGRWEVMNAAGE
                     DVVLLAGYGIHIARPDWQVRLSWWLAELSGTSLLFRAWPYLLVALALAIAARRNLTVL
                     TLVASGLVYEAALFVIAPSVDFRYSTWLVLCTLIAFALHLAQIRPSQNQTSRGRLNTS
                     GSRGVSKSISVPRAI"
     gene            complement(7117..>8297)
                     /locus_tag="VFV99_08315"
     CDS             complement(7117..>8297)
                     /locus_tag="VFV99_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059348.1"
                     /translation="EERATAITVDAIGNVVVAGTCDGVVDFGMTRADCHGSFITKRAY
                     DTGAELWTHVLPNAQVSSVSVDDLGYTIANGSYTGTLEIGGSQFTATGNDPFVVGIDP
                     TGGIDGGAALGLVGTAVSPVGTIEPDGCIYMTGGFHGTMPTPDGTMTNQTGELDGYVS
                     GHYADMNGAWMMRFGGERDQVGRALAVQGEHLAVLAETGAPLHVADQVVSAPAWPASV
                     LMQFSIVGQLQWTRVVPDPSTHLAVARDNSIVVAGIGDACTRLHTYDVAGAVQWTSEC
                     TPLLSTDAIAIGVDGTIVTGGRNFDPTSGELFLAAHDGNGAPIGHVNAQPYPAFERDS
                     SLDGIAIEPSGEVAFIATINHPFDFGNGMLAFSGAHDAVIVKLDSPTGHDGPVVLLRK
                     "
BASE COUNT         1437 a         2736 c         2833 g         1291 t
ORIGIN      
        1 gggccgacga gcacggccat tttttgggcc gggatcaaga ggtcgagatc ggggatgacc
       61 ggccggccaa cgccgtcata ctgcttgcga atgccggaaa gctcgacgtc acacacgctt
      121 tgcgcgtaga gcaaccctca ggccaccgca agtgcccggg atgcaacgct gtggttcgac
      181 ctctgttgcc aaggacaacg ctgcaagatg gcctacgcaa cgtttcctaa cgctaggatt
      241 gccgctaatt gaccgatcgt tcgaggtggg ttaaccgtag aggatctaga tggcccgtcg
      301 tcccgtccgt cgccgctcgt ttggccgtag gcgggcccgc cgtgttcggc agatgcaagc
      361 gctcggcttg ccggcgccgc tgcactcggt ctcgaccccg gtcgatggcg gtgcgctgcg
      421 cgtgctcggc tcgggcgatc aggcgttcca gcagatcctc ggccgcattc gcggagcgca
      481 gaagtcggtc gagattcgcg cgttcttgtg gcgcgatgac gaggctggca acttgctcgg
      541 cgaagcggtg ctcgaagccg ccgatcgcgg cgcacacgtc gtcattcaca aagatcgtat
      601 cgcggccgtg tacgagtaca ccggcggcaa caagcagagc ttcttccaca agcgggtcga
      661 tccgatccgc ggcttccagg cgtggttcct tggcgcggtg tatcgcgcac cgggcagctt
      721 caagcagaag ccgaacgcgc tcgcacagcg catcctcgag cacggcaaca tcaccgtcga
      781 gcacatgcgc aagcgcttcg atcactcgaa gctctacatc atcgacgatc gctacctggc
      841 gaccggttcg atgggcatcg gcgacaacca ccgccacgag tggatcgacg tgatggtcga
      901 agccgaaggt gaagagcacg tcgcgcgtct gcgcgagcgc atgctcggcc acgacgagtt
      961 cgatccgtcg cgcggtctcg acttcttggt gcacagccgc gaggcgcacc gcaagaagag
     1021 ctgcccgatg atcagtcacc ggctcgcgct gatcgaagcg gcgcagcagt cgatcaccgt
     1081 cgagatggcg tacttcaccg accgccggtt cacggcggcg ctcgctcgcg cggtccagcg
     1141 cggcgtcgag gtgaaggtcg tgatcgcaga gtcggcagac gtgctcggca atatcaatcg
     1201 cgcgacatgc gatgcgctga tgcggctgac cggggcgccg cagaatctga gcatcgtgct
     1261 ggtgccgcgg atggttcaca gcaaggtggt cgtcatcgat catcggtgga gcgacgtcgg
     1321 ctcggcgaac ttcacgccgc tgtcgcacgg cgtctacgac gagatcaacc tctacgcgga
     1381 ctgcgagcca ttcgcgcgcg cactcgaagc ggagatcgat tcgcactgtg aagacgggcg
     1441 cgtcgcagat tcgcgactga gctatcgcaa gatgtactca gggctcgagc gcgcgatcat
     1501 cgcgtaccaa tcacgacggg gcggctagtg gcggcgacgg tcgatcccgc agagcgcgcc
     1561 aaacaggagc gccgccgtca gatcatgaac gcggcaaagc acgtgttcgc cgaggccggc
     1621 tatcacggcg catcgatcca cgcgatcatc gagcgcgcgc agatcgcccg cggcacgttc
     1681 tatctctact tcgagagcaa ggcggcggtg ttcgactcga tcctcgacca ggcgatggcc
     1741 gatctgcgtg cgcggatcca tcgcatcgag gtcgacgaca agaacgcgcc ggcgccgcaa
     1801 gtccagctgc gcgagcaggt gatggcgacg ctcgatttcg tcgtacgcga tcgcccgctc
     1861 gcgacgctgc tgttgtcggc ggggcacaca cccgatgccg aggccgccga gcggctcgat
     1921 cagttctttg ccgaagttcg tgatctgctc cgccgtgcga tggagtcggg aatggagatc
     1981 ggcctcttgc gcaaggtcga tccggagctc gcggcgtccg ccatgctcgg catgattcgc
     2041 ggcgtcatcg agcagctgat ccgcgccgag accccacctg cagtcgatgt cgtcgtgagt
     2101 gaggtgctga tggtggcact gcgcggcgtc ctcgcgacgg gttaacctag ggcccgcgtg
     2161 ggtcgggtac tcgcaaatcg gttcgagctg gggccgcgcg tcggcaaagg cagcttgagc
     2221 gtcgtgcacg aggcgatcga tcgcgaatcg agtcgtcgcg tcgcgatcaa gctcgcagcc
     2281 ggcctcgaag acctcgtgat ggtgcgccag cgcttcgatc gcgaggccgc ggcgctgagc
     2341 gtgatctcga gcaagcatgt cgtcgagctg atctcggcgg gcaccgacga agagggactg
     2401 ccgttcctcg tgctcgagcg gctcgacggc agaaacctcc aggaagtgat cgccgaccgc
     2461 gggccaatga agcccgagcg cgtcggtcgc tacatcgcgc aggccgcggc cgcgctcgag
     2521 ctcgcgcatg gcctcggcat cgtgcaccgc gatctcaagc cggcgaacct gttcgcgcac
     2581 gaggtcggcg agaacaccca tcgggtcgtg aaggtgctcg acttcggcct cgtcgtcgac
     2641 accggtgggc cgaccgatcg cctgcgcgac gcgttcggcg gcacgccgct ttacatggca
     2701 cccgaacagg tgcgcggtca gctcacgcgc atcggaccgg cgacagacat ctacgcgatc
     2761 gcgcaggtca cgctgacgct gctcaccggc gagacgtact ggactgcgac gagcgccgaa
     2821 gacgtgatgc gcgagatcga gtcgagcgtg ccgagcaagc cgtcgctgcg ctggccgtgg
     2881 ctgaccgagc cgttcgacca gtggttcttg cgcagcacgc agcgcgtgcc cgagcggcgg
     2941 ttccgcagcg tcgcagcgca ggcggcgcag ctcgtcgagg cattgcgtgg gatccggccg
     3001 cccgatcgca aaacgccagc gccatcgttc gtcgcggcca gcacgctcgc cgcgcgcacg
     3061 ccgacgccaa gcatcgtgcg gctcgccggt caccgcacgc cggtgatcgg gcggcaggtc
     3121 gagtaccgcg acgtcgagca gctgctcgca cgcggtcagg tggtcacgct catcggaccg
     3181 gccggcatcg gcaagacgcg actgtcgcag gcgatctgcg aaaccgtcgg cgagcggttc
     3241 atcgacggca cctggttcgt ctcgctcgtc gcagggcagg gcgagaaggc cgtgctcggc
     3301 ccgatcgcgt cggcggtggc catcgagccc gatgccacgc gatcgttgtt cgagcacgtg
     3361 gtcgacaacc tcgggatgcg ccgcatgttg atcgtgctcg acggtgccga gcacgttgcc
     3421 gactcggcgc gtgtgatcga ccggctcgtc ggcgcgtgtc cggcggtgac gtggctcgtg
     3481 acgagccgcc tgccgctcaa catcgcgaac gagcgtaagt acgtcgtcga ggcgctcgac
     3541 gtaccgcgta ccgatcacat ctcggtcgac gaggcagaaa cgtacggtgg cgtcgcgctg
     3601 ttcgtcgcgt gtgcgcgaga ggtcgccccg atgttcgccc tgaacgaacg caacgccgaa
     3661 gatgtcgtcg cgatctgtcg cgcggtcgcc ggctcgctgc tcgcgatcga gctcgccgcg
     3721 gcacaggtca catcgtcgac cccagcgcag atccgtgcgg gaatcggcga tggcacgcga
     3781 tcggactcgg cagtgcatca ggcgatcatg tcgagctacg cgctgctcgc gcccgatcag
     3841 cagcgcatgt tgcgccagct cgcgatcctg ccggccggtc tcacgttcga tcaggtgaag
     3901 cgcaagctgt cgcacctcag cgacgatccg atgtacgcgg tgctgcgcct cgtgcagacg
     3961 cacctcgcga cctggtcgag cgatgcaccg cgccgcctgc gcatgctcga caccgtgcgc
     4021 gatctcgcgc gtgagcagtc gcgcgaggca ggcgatgacg tggcgctgtg gaacttcgcg
     4081 cgcgagcatg cgtacgcgat cgcgacgagc tcgaacgaga tgacgaccga ggcctggctc
     4141 tcgctcgtcg atgtcgaata cgagaatctg cgcgccgtgc tcggccgcca gttgtccgcc
     4201 gcgcctgccg acgcgatgga cctcgcaggc cggcttgcct attactggta cctccgcggc
     4261 gactacctcg agggcatgca gtggctcgaa gcagcgatcg agcagagctc gggcgaggaa
     4321 ggcgagccgc tcgctcgcgc actgctcggc gcgggtcgca tcgcgctgct cacctgtcgc
     4381 tatgcacggg ccggagagtt gctcgagcgt gcgcgctcga tcgcgctcgc gatcaacgat
     4441 ctgccgggcg aagcaaacgc ggatcagctg ctcggcagcg tcgctcgcga gcttggcgag
     4501 tacacgcgcg cgcacagctt ccatgtccgc agcctcgaga tctgggagcg catcggcgat
     4561 gcgcgcgagg cggcgcgcgc tcgcaactac ctcgcgttcg ccgcgtggat cggcgactac
     4621 gcgggcatgc cgagcgacga gctgctcgag tggtgggacc agaccaacga ggacgagctc
     4681 cgcacactcg gcgacccgga gatcacggtc tggtacctgc tcaaccgcgg cgcgatcctg
     4741 caccaccgcg gcgatccggc tgcgcgcgac gcgctcgggc gcgcatttgc ggaagcgatc
     4801 gcggcgaagt tccacgaggg catcgcgtgg tcgctcgatc tcatcggcag ggtgtcactc
     4861 gaacgcgcgg agttcttgca ggcccgcgca cagcttgccg cagcgctgcg cgtccaccgc
     4921 cgcctcggcg atcgctggcg ctgcgcgagc gtgctcgaag ggctcgccgc agtcgcggtt
     4981 gcgagcgatc ggccagcgcg cggtgcggtc tatctcggcg ccgccgacgc gatccgcgaa
     5041 cagatcggtg cgccggtgcc ggcgtgcgag cggccgatgc tcgcgatgac cgaggcgcgc
     5101 ggcgtcgaca tgatcggcca atcattcgac gccggtcgcg agcgtggccg gcgcacgccg
     5161 ctcgaccaga ccgtcgagct cgcacgcgac gttgtttagc tcgggcgcgg aagtacctgg
     5221 cgcagcgcga tcgtcgcggc ggggcacaac ggcagcgccc agctgatgtt gacgccgttc
     5281 atgccgtcga gcgcgatcgc gcgagtgatg aagtagagca tcgcgaggaa cgcgatcgtg
     5341 agcgagatgc gcgcgacgcg tagcgcgcga ccgccgacct ttgtcacggc cgtcgcgaac
     5401 acgggcacga ccgaggtcgc cgcgccgacg atcgcagcgg cgaacgcgat gtagcccgcg
     5461 atgtagaggt catcctgcgc gccgggcacg ttgtcccagc ggatcgactg gcaaccgtgg
     5521 tcgcagactt caatgcccca caggccaacc gacacggcct gcgccgtgaa ccacgccttc
     5581 acgaatgccg tgaccgccac cagcgcggcc aggccgggca ccagccactg cctcgtcttc
     5641 gtgttcatgt tgcttagata gcacgcggaa ccgaaatcga ttttgaaact ccgcgcgagc
     5701 cactggtgtt caggcgtccg cgagacgtct ggttctgcga cggacggatc tgagccaggt
     5761 gcagcgcgaa cgcgatcagc gtgcagagca cgagccacgt cgagtagcga aagtcgacgg
     5821 acggcgcgat cacgaacagt gcggcttcgt agacgagacc ggatgcgacg agcgtcagca
     5881 cggtgaggtt gcggcgcgcg gcgatcgcga gcgcgagtgc aacgaggagg tatggccatg
     5941 cgcgaaacaa cagcgacgta ccgctcagct ctgcgagcca ccaagatagc cgtacctgcc
     6001 agtcaggccg cgcgatgtga atgccgtagc cggcgagcag cacgacatct tcgcctgcgg
     6061 cgttcatgac ctcccagcga cccgtttcgt acatgcgtag gccgatcagc tcgcgcatga
     6121 cgcgcaggcg cgcgagcagg tacgcgcgcg gctggtcacg gatgagacgc caccacgcag
     6181 agacgaccgc atcgcgctcg gcggctgtcg ccggccgcgt catcaggcgc cgcgggctgt
     6241 cgacgctcgg aaaccaggac agcggactcc acgcgagctc ggcgcggcgc tggatgtgat
     6301 cgtgggcgac gagggggacg cctgcgagca tgcgatcgag cgtcgcgtcc ggcagcggat
     6361 cggcgtagcg cagcatgccg gcgatatcgt acgtcgcgag acttgtcgcg aatggatagc
     6421 gcctctcggt gatcagcgcg cgatttgcga gctgtgcggc accaaacacc acgagcgtga
     6481 tcgcgagacc ggcgagccgg cgacgccaca gcggctggcg cgcgggccac ggcgccacga
     6541 gcacgagcat cggcgcgatc gcgatgatcg cgttgtggcg gagcatcgcc gcgtagaaaa
     6601 agcccgcgag tgccgcgatc cgggcgccac ggcgcgcact cgtcagcgct gcgaacgacg
     6661 cgacgagcac gcctgccatc agcgagtcct tccatacgat ggccatcagc gtgaacaccg
     6721 gcggccacag gaagaccgcg agcgttgcaa gcaccgctat ccacgggcgg agcacacgtg
     6781 tgaggagtgc ctgcatgccg gcgagcagca gggagcactg caggagcacc atcagcagcg
     6841 ggccggcgat gaccttgtcg agcacgcgcc acgtaagcgg catgatcggc agatgccaat
     6901 cggagagcgg catcaggcca cgcgcttgcg cgagctgctc gatcgagtcg tagctcgtga
     6961 agccagggta cgaatacaac gcgatcgcgg tccaccctgc tgcgatgacg acgcaccacg
     7021 ggagccacga tcgccaggca ggggcgacca cgactcctat tgcccgagag cactcgaatc
     7081 tgtcacgcga tcgcaacggc ggaatttgcg tacaggttat ttgcggagca gcaccaccgg
     7141 cccgtcgtgg ccggtcggcg agtcgagctt cacgatcacg gcatcgtgtg cgcccgagaa
     7201 cgcgagcatg ccgttgccga agtcgaacgg atgattgatc gtcgcgatga acgcgacctc
     7261 gcccgatggc tcgatcgcga tgccgtcgag cgacgagtca cgctcgaacg cggggtacgg
     7321 ctgtgcgttg acatggccga tcggagcacc gttgccgtcg tgcgcagcga ggaacagctc
     7381 gccgcttgtc ggatcgaagt tgcggccgcc ggtcacgatc gtgccgtcga caccgatcgc
     7441 aatcgcgtcg gtcgacagca gcggcgtgca ttcgctcgtc cattgcacag cgccggcgac
     7501 gtcgtaggtg tgcagtcgcg tacacgcgtc gccaatgccg gcgaccacga tcgagttgtc
     7561 gcgcgcgacc gcgaggtgag tggacggatc ggggacgacg cgcgtccact ggagctggcc
     7621 gacgatcgag aactgcatca gtacgcttgc cggccatgcc ggcgcggaca cgacctggtc
     7681 ggctacatgc agcggcgccc cggtttcagc gagcaccgcg agatgctcgc cttgcacggc
     7741 tagcgcgcga ccgacctggt cacgctcgcc accaaagcgc atcatccacg ccccgttcat
     7801 atcggcgtag tgaccggaga cgtaaccatc gagctcgccg gtctggttcg tcatcgtgcc
     7861 gtcgggcgtc ggcatcgtgc cgtggaagcc gcctgtcatg tagatgcaac cgtcgggctc
     7921 gatcgtgccg accggcgaca ccgccgtacc cacaaggccg agagcggcac cgccgtcgat
     7981 gccccctgtc ggatcgatac cgacgacgaa cggatcattg cccgtcgcgg tgaactgcga
     8041 cccgccgatc tcgagagtgc cagtgtaact accgttcgcg atcgtgtagc cgaggtcgtc
     8101 gacgctgacg ctcgacacct gggcgttggg cagcacgtgc gtccagagct ccgcgcccgt
     8161 gtcgtacgcg cgcttcgtga tgaacgagcc gtggcaatcg gcgcgcgtca tgccgaagtc
     8221 gacgacgccg tcgcacgttc ccgccacgac gacgttgcca atcgcatcga ccgtgatcgc
     8281 ggtcgcgcgc tcctcgc
//
LOCUS       DASUFX010000212        12919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1421301, whole genome shotgun sequence.
ACCESSION   DASUFX010000212 DASUFX010000000
VERSION     DASUFX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421301"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFV99_08320"
     CDS             <1..499
                     /locus_tag="VFV99_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5059349.1"
                     /translation="MTDFLTLLQRDHHDLETGLDELLKATTVAEIRSALDGVRLGLMA
                     HAEAEDIVLHRALVNAGVPRILDELIGQARSAHLSQEGALSALVCARPGSLEWRERAR
                     RLHELVHSHALFEESTVVPAIRELAPAVYKRLAGLFATERLHQLAMLQPSAPIVLSEL
                     AQAGR"
     gene            complement(496..1188)
                     /locus_tag="VFV99_08325"
     CDS             complement(496..1188)
                     /locus_tag="VFV99_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059350.1"
                     /translation="MTAAADTVLVVEDEAPMRRFLSAALQSHGFRVVEASTLGTAQRI
                     ATEQHPDAILLDLGLPDGDGLTLVRDLRTWSSAPVIVLSARDREDDKVTALDAGADDY
                     LTKPFGTSELLARIRVALRHSRAQRVPDDPVLAVGAIRIDQARHEVNVGERAIHLTPI
                     EYRLLVLLARHPGKVLTHKQLLQDVWGPRTHQTHYLRVHMAALRRKIENDPARPRWIT
                     TEPGVGYRLIDR"
     gene            complement(1185..2261)
                     /locus_tag="VFV99_08330"
     CDS             complement(1185..2261)
                     /locus_tag="VFV99_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX5059351.1"
                     /translation="MADQRPPRTPVRAYVISLITVGVATLVGLVAAPFASHADQAMAY
                     LLAILVAALGGRGAGLTAAVLSAAAFDFFFVEPYHTFAVANRSFLITFGVMVAIGIAI
                     GSLVNRIRVAEAESRERELRAKAEELRSTLLSSVSHDLRTPLAVITGMATSLRDSAQG
                     EQREELDTIVGEARRLSNILTNLLSITKVESGAEPRREWVPLDELIGSSLARLEPELA
                     EHPLTLEVADELAHVDPILTEQLLVNLVENAAKHTPPGCAIEVQARRDGSAALIEISD
                     HGPGLPPGPPEQVFEKFFRGRGVKAGGVGLGLTVCKAIATAHKGRIEASMRDGGGAVF
                     RVWFPDDGAPPRLGDAPELLARAS"
     gene            complement(2280..2498)
                     /locus_tag="VFV99_08335"
     CDS             complement(2280..2498)
                     /locus_tag="VFV99_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059352.1"
                     /translation="MLTEERIIYAMLIVVGGLASASEVAEHGANIGTRATIALLIGCA
                     GIVGLVRSWLQPSTLPRARVFRRRAWRP"
     gene            complement(2501..4393)
                     /locus_tag="VFV99_08340"
     CDS             complement(2501..4393)
                     /locus_tag="VFV99_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX5059353.1"
                     /translation="MPHGHSPTSRRELTLLSLGALGVVYGDIGTSPLYAMNECLLSNK
                     PHAVAPTLENVLGVMSLFFWTLMLIVVVKYLYFVLRADNKGEGGILALAALVAQKTPK
                     AHNRLAIPILLALFGAGLLYGDGVITPAISVLGALEGLSEHSATMTEFIVPITTLILI
                     ALFSIQRFGTHRIGSIAGWVMLLWFVAIGVAGLPHIIARPEVLRAVSPHYAVRFMIEH
                     QFHGFVLLGLVVLCVTGAEALYADMGHFGKTPIRFAWSTVVFPGLLLNYFGQAALFLE
                     HGGNVTNGFYDLVPQGLVIPMTILATFAAVIASQALISGAFSLTNQAVQLGYFPRVKV
                     VHTSHKMEGQIYIPEVNYLLMFACVALVLTFKNSTNLAAAYGIAVTGTMAITSYLYFL
                     VCRHNWNYTWGKALAIFVPFVLIDLTFFSANALKFVDGGWFPLAVGTGVFIIMTTWWR
                     GRNELSKLMEGGTIPDEIFLTDIAETPLPRVSGTAVFMSSGTDGMPNVLLHHVKHNKV
                     LHKQVVLLSVVTENIPFAIGNQSLTLRELGHGFYRVVARVGFMQQPNVPRILARCEKH
                     NLTIVASDTTYYLGRQTLLTSGQSKLARWRKLVFAFLSRNARTPTAFFNLPPNRVVEL
                     GLQIEL"
     gene            complement(4537..6567)
                     /locus_tag="VFV99_08345"
     CDS             complement(4537..6567)
                     /locus_tag="VFV99_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX5059354.1"
                     /translation="MSHAGAHVSGSHRGDQTGAHGHAHASHGQGLHPHTRKQLTWLSL
                     GALGVVYGDIGTSPLYALKECFTHENPHRAAVESGLLTFNGTQPVIQRVIETDNILGL
                     LSLFVWSLTLVVVVKYLVFVLRADNKGEGGTLALAALVAQKAPRPSKTRLAIPILLAL
                     FGTGLLFGEGIITPAISVMSAVEGLKEQSPSFGKLIVPISAGILIGLFYVQRYGTGRI
                     GSVFGWIMLLWFLSIAAAGMPYIVQYPQVLAALSPHYGIVFLATHGMHGFLLLGSVVL
                     CITGCEALYADMGHFGRTPIRIAWTWVVFPGLLLNYFGQGALYLHEGAKVSHPFYDLV
                     SGTPLLIPMVILATLAAIIASQALISGAFSLANQAVQLGYLPRVKVVHTSSKAEGQIY
                     IPEINWLLMVACLGLVFAFRSSTALAAAYGIAVTGTMSITSFLFFRICRQNWGWSKRA
                     ALGLYLPLIIIDYSFFSANLVKLGDGGWFPLAIGVAMFTIMTSWWRGRMELSKMMETG
                     TIPDELFLADIAETHIHRVAGTAVFMSSGTDGMPNVLLHHVKHNKVLHKQVVLLSVVT
                     ENVPFAIGNQSLTVRDIGQGFYRVIAHVGFMQQPNVPKILNRCEKHHLTIAQSDTTYY
                     LGRQTLLTTGKSGMAKWRKLLFSFLARNARTPAMFFRLPPNRVVELGLQIEL"
     gene            6694..7884
                     /locus_tag="VFV99_08350"
     CDS             6694..7884
                     /locus_tag="VFV99_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5059355.1"
                     /translation="MNLNTWRGLRSVLIIAALIAAAACDKGKGGGSGSGGSGGAKPAS
                     GSIVIGHFASMTGPQATFGISTDQAIRLAIKEKNAKGGVKGRQVELVTIDDAGKQSEA
                     ATAVTRLINDHKAVAVLGEVASSLSLAGGPIAQKSKIPMISPSSTNPDVTDVGDYIFR
                     VCFLDDFQGWVDAKFAKENLKATKAAILYDQAQAYSSGLADYFDKAFKELGGTIVTKQ
                     AYTGGNLEISSQLQSIKGSGAEVVFLPGYYSDAGTIIRKAKEAGITAKFLGGDGWDSE
                     ELPKIAGDAINGNFFSNHYAPQEDRPEVKNFVENYKKEYGKTPDGLAALGYDAALVLF
                     DAMERAPSLDGPALRDAIAATKNFTGVTGTFSIDENRNAMKSAVIVEYKDGKQTMSAR
                     IEKK"
     gene            7881..8846
                     /locus_tag="VFV99_08355"
     CDS             7881..8846
                     /locus_tag="VFV99_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5059356.1"
                     /translation="MSDSFTASDLLQTTINFLAVGGLYALIALGYTLVYGILRFINFA
                     HSDIVAWGGWIALTVASGAGWLASGQSPAFALPLVLLATMALCAAMGVTIERLAYRPL
                     RKAPRLNVLITAIGVSLFLQNVGLLNKVFGNRPSTISALLSNDVLATVGGVHLRLYDM
                     LTILLAVVLVIVLERIVYGTRMGRAMRAVAFDERTAALMGVNVNIVVSVTFALGSALA
                     AAGGILFALRNDLRMTADTSWVLLGLKAFVAAVVGGIGNVRGAAAGALLIAFVEVFVH
                     RYVTAAFPDKPWVGQLQDIYVFGILILVLIFKPSGLLGKATVEKV"
     gene            8843..9958
                     /locus_tag="VFV99_08360"
     CDS             8843..9958
                     /locus_tag="VFV99_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5059357.1"
                     /translation="MKSLQDRVDRLRPGLPITVGKSVLPFAIAILLAFTMDKLIGPAV
                     GATWSKILLDVGIAILLAGSLNIVNGYAGQFSIGHAGFMLVGGYLAAWLTFYGSIHWF
                     GVKTPVGGFLGNGQLLFLGGCILGGLCASVFGVIVGLPSLRLRGDYLAIVTLGFGEIV
                     RVLGQITDRQPKRIDDVKKIPMSDLLNNLGGAEGFSQIPGYTTLFFVYFFAFVLLVLA
                     YRLKYSSKGRALLAVRENEIAAEAMGIDTTRAKVIAFVFSAFFAGIGGSLYAHQVGAL
                     DPKELGFMKSIDLVIIVVIGGMGSITGVVIGATILVVLPEIFRQFADYRMIAYALALI
                     TVMILRPQGIMGVKELWETSLWRKQVKALRAKLRGGK"
     gene            9994..10833
                     /locus_tag="VFV99_08365"
     CDS             9994..10833
                     /locus_tag="VFV99_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5059358.1"
                     /translation="MTRGALDIKKLSLAFGGLKAVSEFSLSLPVGGLYGLIGPNGAGK
                     TTVFNLLTGVYKPDVGSVELSGLRIDGKKPNQIARAGMARTFQNIRLFGELSVIDNVR
                     TAAGVRTKSGLFRTLLRTPLHVAEERAITERCIELLDVLGITHRQDEQAKSLPYGDQR
                     RLEIARALATEPKVLLLDEPVAGMNTQEKREMRGLIQSLREKFGVSILLIEHDMGLVM
                     DICEQITVIDHGVTIAVGSPKQIQDDPAVIEAYLGVPEENDPIPRPEEAPKTDAPEAA
                     EAP"
     gene            10839..11600
                     /locus_tag="VFV99_08370"
     CDS             10839..11600
                     /locus_tag="VFV99_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5059359.1"
                     /translation="MLEIDGIDVHYGGIHALKGVTMKVEKGEVVTLIGANGAGKTSTL
                     RAISGLVKPSAGRVRFNGEEIVSAGKSNQPRWKKWVEGDGIGAHAIVARGLVHAPEGR
                     GIFANMTVAENLEIGAFLRNDAAQVRKDRDHALSLFPRLKERLAQNAGTLSGGEQQML
                     AIARALLSRPKLLLLDEPSLGLAPQIVALIFKIVKTIAADGTTILLVEQNAHMALGVA
                     NRAYVLEVGQIVLDGDAKELAKNDQIRKAYLGIHD"
     gene            11753..12001
                     /locus_tag="VFV99_08375"
     CDS             11753..12001
                     /locus_tag="VFV99_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059360.1"
                     /translation="MPNDNKNQNQGQGQKKQDIEREREDVKRVGNDNDNDLDRDEDQE
                     VTQRNPRQGDQGQQQGSQKMGNQGQMGNQGQDRDRNKP"
     gene            11998..12489
                     /locus_tag="VFV99_08380"
     CDS             11998..12489
                     /locus_tag="VFV99_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5059361.1"
                     /translation="MSIIELIKREHDEVGYLFDHLLALARDHRHANAAARCAARLVTL
                     VRVHSHAEERVFYEALRTKTPELKAFALAGPHEHEVIDTTIDKLLVRQPGDDEYRIIV
                     RVARDLFQMHARDEEETDILPLVAEAFPADEQEALAVDMLAEEARIRPQILRLIGAPA
                     RAA"
     gene            12560..>12919
                     /locus_tag="VFV99_08385"
     CDS             12560..>12919
                     /locus_tag="VFV99_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5059362.1"
                     /translation="MHNRSGERRTQWVVALTAVMMIGELIVGYLTGSVALKADGWHMG
                     THVGALGLTLVAYWYARKHAGNDTFSFGTGKVYALAGFTSGIVLAIVALALAVEGVEK
                     LVDHPSVNYDEALPIAAL"
BASE COUNT         2378 a         4094 c         4257 g         2190 t
ORIGIN      
        1 catgaccgat ttcctgacgt tgctgcagcg cgaccatcac gatctcgaaa ccggcctcga
       61 cgagctcctc aaagcaacga cggtcgccga aattcgcagc gcactcgacg gcgtacggct
      121 gggcctcatg gctcatgccg aagcagaaga catcgtcctc catcgcgcgc tcgtcaacgc
      181 gggcgtgccg cggattctcg acgagctgat cggtcaggcg cgctccgcac acctctccca
      241 ggagggcgcg ctctcggctc tggtctgtgc gcgtcccgga tcgctcgagt ggcgagagcg
      301 tgcgcgacgg ctgcacgagc tcgttcacag ccacgcgctc tttgaagaga gcaccgtggt
      361 ccctgcgatc cgcgagctcg cgccggccgt gtacaagcgt ctcgcgggcc tattcgcgac
      421 cgagcgcctc catcagctcg cgatgctcca gccgtcggcg ccgatcgtcc tgagcgagct
      481 cgcgcaggct ggtcgctagc ggtcgatcaa ccgatagccg acgccaggct ctgtcgtgat
      541 ccagcgcggg cgcgccggat cgttctcgat cttgcggcgg agcgccgcca tgtgcacgcg
      601 gagatagtgc gtctggtgcg tgcgcgggcc ccacacgtcc tgcaacagct gcttgtgcgt
      661 gagcaccttg cccgggtgcc gcgcgagcag gacgagcagc cggtactcga tcggcgtcag
      721 gtggatcgcg cgctcgccga cgttgacctc gtggcgcgcc tgatcgatcc ggatcgcgcc
      781 gaccgcgagc accggatcgt cgggaacgcg ctgggcacgc gagtggcgta gtgcgacgcg
      841 gatgcgtgcg agcagctcgc tggtgccgaa cggcttcgtc aggtagtcgt ctgcgcccgc
      901 gtcgagtgcg gtgaccttgt cgtcctcgcg atcgcgcgcg gacagtacga tgactggtgc
      961 cgacgaccac gtgcgaaggt cgcggaccag cgtcaggccg tctccgtcgg gcaggccgag
     1021 atcgaggagg atcgcgtcgg gatgctgctc ggttgcgatt cgctgcgcag tgccgagcgt
     1081 gcttgcctcg acgacacgaa agccgtggct ctgcagtgcg gccgatagga atcggcgcat
     1141 cggcgcctcg tcctcgacga cgagcacggt gtcggcggcc gccgtcatga tgccctcgcg
     1201 agcagctccg gcgcatcgcc gaggcgaggc ggcgcaccgt cgtcgggaaa ccatacgcgg
     1261 aagacggcac cgccgccgtc gcgcatgctc gcttcgatac gacctttgtg cgcagtcgcg
     1321 atcgctttgc acacggtgag gccgaggccg acgccgccgg ctttgacgcc acggccgcgg
     1381 aagaatttct cgaacacttg ctcgggcggt ccgggcggca ggccagggcc atgatcgctg
     1441 atctcgatca gcgcggccga gccgtcacgc cgcgcctgca cctcgatcgc gcagccgggc
     1501 ggtgtgtgct tcgccgcatt ctcgaccagg ttcacgagca gctgctcggt gaggatcgga
     1561 tcgacatgcg cgagctcgtc ggcgacctcg agcgtgagtg gatgctcggc gagctcgggc
     1621 tcgagccggg caagcgacga accgatcagc tcgtcgagcg gcacccactc gcggcgtggc
     1681 tccgcgccgg attcgacctt cgtgatcgag agcaggttcg tcaggatgtt gctgagccgg
     1741 cgcgcctcgc caacgatcgt gtcgagctct tcgcgttgct cgccttgcgc gctgtcgcgc
     1801 agcgacgttg ccatgccggt gatcaccgcg agtggggtgc gcagatcgtg tgagaccgac
     1861 gatagcagcg tgctgcgcaa ctcctcggcc ttcgcgcgta gctcgcgctc tcgactctcg
     1921 gcctcggcga cccggatgcg attgacgagg ctgccgatcg cgatgccgat cgcgaccatc
     1981 acgccgaacg tgatcaagaa gctgcggttg gcgacggcga acgtgtgata gggctcgacg
     2041 aagaagaagt cgaacgccgc agccgacagc actgcggcgg tcagacctgc acctcggcca
     2101 ccgagcgcgg cgaccaggat cgcgagcagg taggccatcg cttgatcagc gtggctcgca
     2161 aacggcgcgg cgacgagccc cacgagcgtc gcgacgccga ccgtgatcag cgagatcacg
     2221 tacgcgcgaa caggcgtgcg gggagggcgc tggtcagcca cgcgcggaac gtaccgcctt
     2281 cacggtcgcc atgctcgtcg acggaaaacc cgcgcgcgag gcagggtgga tggctgcagc
     2341 caactgcgca ccagtcctac gattccggcg cagccgatca ggagcgcgat cgttgcccgc
     2401 gtgccgatgt tcgcgccgtg ctccgcgact tcgctcgccg acgcaagacc gccgacgacg
     2461 atcagcattg cgtagatgat gcgctcctcc gtcagcatgc tcagagctcg atctgcaaac
     2521 caagctccac gactcggttt ggtggcaggt tgaagaatgc ggtcggcgtg cgcgcgttgc
     2581 gggagaggaa cgcaaacacg agcttgcgcc agcgcgcgag cttcgactgg ccgctcgtca
     2641 gtagcgtctg gcggcctaag tagtacgtcg tatccgacgc cacgatcgtt agattgtgct
     2701 tctcgcagcg cgcgaggatg cgcggcacgt tcggctgctg catgaacccg acgcgtgcga
     2761 cgacgcggta gaagccatgg ccgagctcgc gcagcgtgag tgactggttg ccgatcgcga
     2821 acgggatgtt ctcggtgacg acactcagca gcacgacttg cttgtgcagc accttgttgt
     2881 gcttgacgtg atggagcagc acgttcggca taccgtcggt gcccgacgac atgaacaccg
     2941 cggtgccact cacgcgcggc agcggagtct ccgcgatatc ggtgaggaag atctcgtcgg
     3001 ggatcgtgcc gccctccatc agcttcgaca actcgttccg gccacgccac cacgtcgtca
     3061 tgatgatgaa cacgccggtg ccgaccgcga gcgggaacca accgccgtcg acgaacttca
     3121 gcgcgttcgc cgagaagaac gtgaggtcga tgagcacgaa cgggacgaag atcgcgagcg
     3181 ccttgcccca ggtgtagttc cagttgtgcc ggcacacgag gaagtacaag tacgacgtga
     3241 ttgccatcgt tccggtgacc gcgatgccgt acgcggcggc gagattcgta ctgttcttga
     3301 atgtgagcac gagcgccacg cacgcgaaca tgagcagata gttcacctcg gggatgtaga
     3361 tttgcccttc catcttgtgc gaggtgtgca ccaccttgac gcgcgggaag tagccgagct
     3421 gcacggcctg gtttgtcagc gagaacgcgc cggagatcag tgcttgcgac gcgatgaccg
     3481 cggcgaacgt tgcgaggatc gtcatcggaa tgacgagtcc ttgcggcacg aggtcgtaga
     3541 agccattcgt cacgttaccg ccgtgctcga gaaacaatgc ggcctgtccg aagtagttca
     3601 ggagcaggcc cgggaacacg acggtcgacc acgcgaaccg gatcggtgtc ttgccgaagt
     3661 gacccatgtc tgcatacagc gcctcggcac cggtcacgca gagcacgacg aggccgagca
     3721 gcacgaagcc gtggaactgg tgctcgatca tgaacctcac cgcgtagtgc gggctcacgg
     3781 cgcggaggac ctcgggacga gcgatgatgt gcggtaagcc ggcgacgccg atcgcgacga
     3841 accagagcag cattacccag cccgcgatcg agccgatgcg gtgcgtgccg aaacgctgga
     3901 tcgagaacag cgcgatcaag atgagcgtcg tgatcggcac gatgaattcg gtcatcgtcg
     3961 ccgagtgctc ggacagacct tcgagcgcgc cgagcaccga gatcgccggc gtgatcacac
     4021 cgtcaccgta gagcaggccg gcgccgaaca gcgcgagcag gatcgggatc gcgagacggt
     4081 tgtgtgcttt gggcgtcttc tgcgcgacca gtgcggcaag cgcaagaatg ccgccttcgc
     4141 ctttgttgtc agcgcggagc acgaagtaca ggtacttcac gaccacgatc agcatcagcg
     4201 tccagaagaa cagcgacatc acgccgagta cgttctcgag cgtcggcgcg accgcatgcg
     4261 gcttgttcga caacaggcat tcgttcatcg cgtagagcgg tgacgtgccg atgtcgccgt
     4321 agacgacgcc gagcgcgccg agcgagagga gcgtgagctc tctccggctc gtcggcgagt
     4381 ggccgtgcgg cacgtgagtg gcggtcgttc gatgcgtcct aggatgcgag gacgcggcga
     4441 cgctttcgct catttaggta gctcgctcgg aactaccacg cgtgatttgg cacgacgcca
     4501 atcaccgcgt gtcgcaccca aatcttgatg cgatctttat agctcgatct gaagtccgag
     4561 ctcgactacg cgatttggcg gaaggcggaa gaacattgcg ggagtccgcg cgttacgcgc
     4621 caagaacgag aacagaagct tgcgccactt cgccatgccg gactttcccg tagtgagcag
     4681 tgtctgccgg cctaggtaat acgtcgtatc gctctgagcg atcgtcaggt ggtgcttctc
     4741 gcagcgattg agaatcttcg gaacgttcgg ctgctgcatg aagccgacgt gcgcgatcac
     4801 gcgatagaag ccctgaccga tatcgcgcac ggtgagcgac tgattgccga tcgcgaacgg
     4861 cacgttctcg gtcacgaccg agagcagcac gacctgcttg tgcagcacct tgttgtgctt
     4921 cacgtgatga agcagcacgt tcggcatgcc atcggtgccc gatgacatga acaccgcggt
     4981 gccggccacg cgatggatgt gcgtctcggc gatgtccgcg aggaacagct cgtcgggaat
     5041 cgtcccggtc tccatcatct tcgacagctc catgcgaccg cgccaccagc tggtcatgat
     5101 ggtgaacatc gcgacgccga tcgccagcgg gaaccaaccg ccatcgccga gcttgacgag
     5161 gttcgccgag aagaacgagt agtcgatgat gatcagcggc aggtagaggc cgagcgccgc
     5221 acgcttcgac cagccccagt tctggcggca gatcctgaag aacaagaacg acgtgatcga
     5281 catcgtgccg gtgaccgcga tgccgtacgc cgcggcgagc gccgtgctcg agcggaacgc
     5341 gaacacgagg ccgagacacg cgaccatcaa caaccagttg atctcgggga tgtagatctg
     5401 gccttcggcc ttgctcgacg tatgcacgac cttgacgcgc ggcaggtagc cgagctgcac
     5461 ggcctggttc gcgagcgaga acgcgccgga gatcaacgct tgcgacgcga tgatcgcggc
     5521 gagcgtcgcg aggatgacca tcgggatgag cagcggcgtg ccgctgacga ggtcgtagaa
     5581 cggatgcgag accttcgcac cctcgtgcag gtagagcgca ccttggccga agtaattgag
     5641 gaggaggcca gggaacacga cccatgtcca cgcgatgcga atcggcgtgc gaccgaagtg
     5701 acccatgtcg gcgtagagcg cttcgcagcc ggtgatgcac aggacgaccg agccgagcag
     5761 caagaagccg tgcatgccgt gcgttgcgag gaacacgatc ccgtagtgcg gcgagagcgc
     5821 ggcgagcacc tgcgggtact gcacgatgta cggcatgccg gcggccgcga tggacaagaa
     5881 ccacagcagc atgatccagc caaacaccga gccgatgcgg ccggtgccat agcgctggac
     5941 gtagaagagg ccgatgagga tgccggcgga gatcggaacg atcagcttgc cgaagctggg
     6001 actctgctct ttgaggcctt cgacggcgct catcaccgag atcgccggcg tgatgatgcc
     6061 ttcgccgaac agaaggccgg tgccgaacaa cgcgagcagg atcggaatcg cgagtcgcgt
     6121 cttcgacggc cgcggcgctt tctgcgcgac gagcgcggcg agcgcgagcg tgccaccttc
     6181 gcctttgttg tcggcgcgca gcacgaacac caggtacttc acgaccacga ccagcgtcag
     6241 cgaccagacg aacagcgaca gcaggccgag gatgttgtcg gtctcgatca cccgctggat
     6301 gacgggttgc gtgccgttga acgtcagcag gcccgattcg accgcggcgc ggtgcgggtt
     6361 ttcgtgagtg aaacattctt tcagcgcgta cagcggcgag gtgccgatgt cgccgtagac
     6421 gacgccgagc gcgcccagcg acagccatgt cagctgcttc cgggtgtggg gatgcaggcc
     6481 ctggccatgt gacgcgtgcg cgtgaccatg tgcccctgtc tgatcgccgc gatggcttcc
     6541 cgagacgtgc gcgccagcgt gactcaacta gctctgtggt agaccctgcc ggtaaagatt
     6601 tccatcgatc ggatcaagat ctcatcaaca tcgtggggtt gacacggtgc cgggcctcgg
     6661 atatcgcatg cccctatacc tctggagaac gccgtgaatc tgaacacttg gagaggcctc
     6721 cgttccgtcc tgatcatcgc tgctctgatc gcagctgctg cgtgtgacaa gggcaagggc
     6781 ggcggtagcg gtagcggcgg aagcggcggt gcgaaaccgg cgagcggctc gatcgtgatc
     6841 ggccacttcg cgtcgatgac cggaccgcaa gcgacgttcg gtatctcgac cgaccaggca
     6901 attcgcctgg cgatcaaaga gaagaacgcg aagggcggcg tgaagggtcg ccaggtcgag
     6961 ctcgtcacga tcgacgacgc cggcaagcag agcgaagccg cgaccgcggt cacgcgtctc
     7021 atcaatgatc acaaagcggt cgcggtgctc ggcgaggtcg cctcgtcgct ctcgctcgcg
     7081 ggcggcccga tcgcacagaa gtcgaagatt ccgatgatct cgccgtcgtc gacgaacccg
     7141 gatgtcaccg acgtcggtga ctacatcttc cgcgtctgct tcctcgacga cttccagggt
     7201 tgggtcgacg cgaagttcgc gaaggaaaac ctcaaggcca cgaaggccgc gatcctctac
     7261 gaccaggcgc aagcgtactc gagtggcctc gccgattact tcgacaaggc gttcaaggag
     7321 ctcggcggta cgatcgtcac gaagcaggcg tacaccggcg gcaacctcga gatcagctcg
     7381 cagctgcagt cgatcaaggg ctcgggggcc gaggtcgtgt tcctgccggg ctactactcc
     7441 gatgccggca cgatcatccg caaggcgaag gaagcgggca tcacggcgaa gttcctcggc
     7501 ggcgacggct gggacagcga agagctgccc aagattgcgg gcgacgcgat caacggcaac
     7561 ttcttctcga accactacgc gccccaggaa gatcgccccg aggtgaagaa cttcgtcgag
     7621 aactacaaga aggaatacgg caagacgcca gacggcctgg ccgcgcttgg ctacgacgct
     7681 gcgctcgtgc tgttcgacgc gatggagcgc gcgccgtcgc tcgacggtcc cgcgctgcgc
     7741 gacgcgatcg ccgcaacgaa gaacttcacg ggcgtgaccg gcacgttctc gatcgacgag
     7801 aaccgcaacg cgatgaagtc ggccgttatc gtcgagtaca aagacggtaa gcagacgatg
     7861 tctgcgcgta tcgagaagaa gtgagcgata gcttcacagc ttccgatctc ctccagacga
     7921 cgatcaactt cctggccgtc gggggcctct acgcgctgat cgcgctcggc tacacgctgg
     7981 tctacggcat cctgcgcttc attaacttcg cgcactccga catcgtcgcg tggggcggct
     8041 ggatcgcgct aaccgtcgca tcgggcgccg gctggctcgc gagcgggcag tcgccggcgt
     8101 tcgcactgcc gctcgtgctg ctcgcgacaa tggcgctgtg cgcggcgatg ggcgtcacga
     8161 tcgagcggct cgcgtatcgg ccgctgcgca aggcgccgcg cctcaacgtg ctgatcacgg
     8221 cgatcggcgt gtcgctgttc ctgcagaacg tcggcctgct caacaaggtg ttcggcaatc
     8281 gtccgtcgac gatctcggcg ctgctctcga acgacgtgct tgcgacggtc ggcggcgtgc
     8341 accttcgcct ctacgacatg ctgacgatcc tcctcgcggt cgttctcgtc atcgtgctcg
     8401 agcgcatcgt ctacggcacg cggatgggac gtgcgatgcg cgcggtcgca ttcgacgagc
     8461 gcaccgccgc gctgatgggt gtcaacgtga acatcgtcgt atccgtcacg ttcgcgctcg
     8521 gttcggcact tgcggccgcg ggcggcatcc tgttcgcgct gcgcaacgac ctccgcatga
     8581 cggcggacac gtcgtgggtg ctactcggcc tcaaggcgtt cgtcgccgca gtcgtcggcg
     8641 gcatcggtaa cgtgcgtggc gcggcagccg gcgcgctgct catcgcgttc gtcgaggtgt
     8701 tcgtccatcg ctacgtcacc gcagcgttcc ccgacaagcc gtgggtcggt cagctccagg
     8761 acatttacgt gttcggcatc ctgatcctgg tcctcatctt caagccgagc ggtctgctcg
     8821 ggaaggcgac ggtcgagaag gtatgaagtc gctgcaagac cgcgtcgacc ggctgaggcc
     8881 cgggctgccg atcactgtcg gcaagtcggt gctgccgttc gcgatcgcga tcctgctcgc
     8941 gttcacgatg gacaagctga tcgggccggc cgtcggcgcg acgtggagca agatcctgct
     9001 cgacgtcggc atcgcgatct tgctcgccgg ctcgctcaac atcgtcaacg gctacgccgg
     9061 tcagttctca atcggtcacg ccgggttcat gctcgtcggc ggctacctcg ccgcgtggct
     9121 cacgttctac ggctcgatcc attggtttgg cgtcaagacg ccggtcggtg gtttcctcgg
     9181 caacggccag ttgttgttcc tcggcggctg catcctcggc ggcctttgcg cgagtgtgtt
     9241 tggcgtgatc gtcggcttgc cgtcgctgcg actgcgcggc gactacctcg cgatcgtcac
     9301 gctcggtttc ggcgagatcg tccgcgtgct cggccagatc accgaccgcc agccgaagcg
     9361 catcgacgac gtgaagaaga tcccgatgag cgatctgctc aacaacctcg gcggcgccga
     9421 gggcttctcg cagattcccg gctacacgac gctgttcttc gtctacttct tcgcgttcgt
     9481 gctgctcgtg ctcgcgtatc ggctcaagta ctcgagcaag ggccgcgcgc tgctcgcggt
     9541 gcgcgagaac gaaatcgccg ccgaggcgat gggtatcgac accacgcgcg caaaggtgat
     9601 cgcgttcgtg ttctccgcgt tcttcgccgg catcggcggc tcgctgtacg cgcatcaggt
     9661 cggcgcgctc gatccgaaag agctcggctt catgaagtcg atcgacctgg tgatcatcgt
     9721 cgtgatcggc ggcatgggct cgatcaccgg tgtcgtcatc ggtgcgacga tcctcgtcgt
     9781 gctgcccgag atcttccggc agttcgccga ctaccgcatg atcgcgtacg cactagcgct
     9841 catcacggtg atgatcctgc ggccgcaagg aatcatgggc gtcaaagagc tgtgggagac
     9901 gtcgttgtgg cgcaagcagg tcaaggcgct gcgcgcgaag ctgcgaggcg gcaaatgacc
     9961 ggcggcactg tggagcgcag tcccgccggt tctgtgacac ggggcgccct cgacatcaag
    10021 aagctgtcgc tcgcgttcgg cggcctcaag gcggtcagcg agttctcgct gtcgctgccc
    10081 gtcggcggtc tctacggcct catcggtccg aacggtgccg gcaagacgac ggtgttcaac
    10141 ctcctgaccg gcgtgtacaa gccggacgtc ggcagcgtcg agctctctgg actgcgcatc
    10201 gacggcaaga agccgaacca gatcgcgcgc gcgggcatgg cgcgtacgtt ccagaacatc
    10261 cggttgtttg gcgagctctc ggtgatcgac aacgtgcgca ccgcggcggg cgtgcgtacg
    10321 aagagcggcc tgttccgcac gttgctccgc acgccactgc acgtcgccga agagcgcgcg
    10381 atcaccgagc gctgcatcga gctcctcgat gtcctcggaa tcacccatcg ccaggacgag
    10441 caggcgaaga gcttgcccta tggcgatcag cgccggctcg agatcgcacg cgcgctcgcg
    10501 accgagccga aggtgctgct gctcgacgag ccggtcgccg gcatgaacac gcaggagaag
    10561 cgcgagatgc gcggcctcat ccagtcgctg cgcgagaagt tcggcgtctc gatcttgctg
    10621 atcgagcacg acatgggcct cgtcatggac atctgcgagc agatcaccgt gatcgaccac
    10681 ggcgtgacga tcgcggtcgg ttcgcccaag cagatccagg acgacccggc ggtgatcgag
    10741 gcctacctcg gcgttcccga ggagaacgat cccattccgc gccccgaaga agcgccgaag
    10801 accgatgcgc ccgaagccgc ggaggctccg taatggcgct gctcgagatc gatgggatcg
    10861 acgtccacta cggcggtatc cacgcgctca agggcgtgac gatgaaggtc gagaagggcg
    10921 aggtcgtcac gctgatcggc gcgaacggcg ccggcaagac gtcgacgctg cgcgcgatct
    10981 cggggctcgt gaagccgagc gcgggcaggg tgcgcttcaa cggcgaagag attgtatcgg
    11041 cgggaaagag caatcagcca cgctggaaga aatgggtcga gggcgatggc atcggcgctc
    11101 acgcgatcgt cgcgcgcggg ctcgtccatg ctcccgaggg tcgcggcatc ttcgcgaaca
    11161 tgacagtcgc cgagaatctc gagatcggcg cattcttgcg caacgacgcc gcgcaggtgc
    11221 gtaaggaccg cgatcacgca ctgtcgctgt tcccgcgcct gaaggagcgc ctcgcgcaaa
    11281 acgcaggcac cctgtcgggc ggtgagcagc agatgctcgc gatcgcccgc gcgctgctgt
    11341 cgcggccaaa gctgttactc ctcgacgagc cgtcgctcgg tcttgcgccc cagatcgtcg
    11401 cgctgatctt caagatcgtg aagacgatcg cggccgacgg cacgacgatc ctgctcgtcg
    11461 agcagaacgc acacatggct ctcggtgttg cgaatcgtgc gtacgtgctc gaggtcggtc
    11521 agatcgttct cgatggcgat gcgaaagagc tcgcgaagaa cgatcagatc cgcaaggcgt
    11581 atctcggcat tcacgactaa cgaattgcct gagcgtcggc tgcgccgtgc gcctacgccc
    11641 gttgaggcaa cgccggtcgc cgtatgcgtc ggatctgtaa ccgatgcgtg tcgtcgcacc
    11701 ggcactacgg ttgcaacctc tccgctacac aacccaaccg gagcgggaca ctatgccgaa
    11761 cgacaacaag aaccagaacc aaggtcaggg tcagaagaag caggatatcg agcgcgagcg
    11821 tgaggacgtg aagcgcgtcg gtaacgacaa cgacaatgat ctcgatcgcg acgaggacca
    11881 agaggtcact cagcgtaacc cgcgccaggg cgatcagggt cagcaacagg gatcgcagaa
    11941 gatgggcaac cagggccaga tgggcaacca aggccaggac cgcgatcgca acaagccatg
    12001 agcatcatcg agctgatcaa acgcgagcac gacgaggttg gttacttgtt tgaccacctc
    12061 ctcgcgctcg cgcgcgatca tcgccacgcc aatgcagcgg cacgttgcgc cgcgcggctg
    12121 gtcacgctcg tgcgcgtcca ttcacacgcg gaagagcgcg tgttctacga agcgctccgt
    12181 acgaagacac cggagctcaa ggccttcgca ctcgcgggac cacatgaaca cgaggtcatc
    12241 gacaccacga tcgacaagct gctcgtgcgc cagcccggtg acgacgagta ccgcatcatc
    12301 gtgagggtgg cgcgcgacct gttccagatg cacgcacgcg atgaagagga aactgacatc
    12361 ctgccgctgg tcgccgaggc gttccccgcc gacgagcagg aagcccttgc cgtcgacatg
    12421 ctcgccgaag aggcgcggat tcgcccgcaa attctccgtc tgatcggcgc ccccgcacgc
    12481 gcggcctgat ctgctaagta ttgggggctt tatggccacc cacgacctct cagcgcatgc
    12541 ccatcacacc gatgacagca tgcacaaccg gtcgggtgag cgccgcacgc agtgggtcgt
    12601 cgcgctgacg gccgtgatga tgatcggcga gctgatcgtc ggctatctga ctggctcggt
    12661 cgcgctcaag gcggatggct ggcacatggg cacgcacgtc ggcgcgctcg gcctcacgct
    12721 cgtcgcgtac tggtacgcac gcaagcacgc gggcaacgac accttcagct tcggtaccgg
    12781 caaggtctac gcgctcgccg gtttcacgag cggcatcgtg ctcgcgatcg tcgcgctcgc
    12841 gctcgcagtc gaaggcgtcg agaagctggt cgatcacccg tccgtcaact acgacgaagc
    12901 gctaccgatc gccgcgctc
//
LOCUS       DASUFX010000213         7782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1006072, whole genome shotgun sequence.
ACCESSION   DASUFX010000213 DASUFX010000000
VERSION     DASUFX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006072"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2488
                     /locus_tag="VFV99_08390"
     CDS             <1..2488
                     /locus_tag="VFV99_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059363.1"
                     /translation="RATVASSPATAGFAETQPARDVTATDPARAKQRLATDPTMQSDP
                     TIDGPMAATLPGPHGVADLPTLQRIDDSAYAIGEELARGGMGKILFARDRRLRRDVVI
                     KVMRRESGRVDPRFEREALITARLQHPSIVRVYDAGVFGDGRAFYAMERVRGRSLDVV
                     IADAKTVRDRLALLPHAIAVCDAIAYAHSEGVLHRDLKPHNVLVGPFGETVVIDWGLA
                     KDLRTSQAESASEERPADPDRGSSGDMLTVVGAVLGTPSYMAPEQARGEASDERTDIY
                     ALGALLYTSLSGEPPHRGRTADEVIELVSGGKRVPLGKREPGVPPELAAIVERAMAHE
                     PQARFASALELAEELRRFAAGKLVASHTYSLGQLVRRWIRRYRVTLAVAAVALVILAV
                     GGVLAVNNVMTERDFANTERLKAEAAEDRANTKYDSLLYGLAKKALSTDPSHAAAWLK
                     LMSDRGLSWDKTQKVAIDAARLGLARELRGHAQDIEHVAVTPDGKYVITGSDDSTARI
                     WNLANDALVAELKGHDGPIETMTLSSDGKYLATAGTDGDVRLWEIPSGTGRRLQGHHN
                     TVRGVSFSPDNTKLASTGEDGTLFIWDVTSAASKELLKHTAGLRPVIWYDNTTVIVGG
                     FDGKMGRYDVTTGKGSMVQAHDAELRCLALSPDRRYLISGDENGLVALWTGDGKRLRS
                     LGRHTDVTRDVMFTPDGKFALSAGGDPIVRVYPIPDGKAYELTGHENGIKGLALDRDG
                     KLVASAGLDNTVRVWTIDGKPFRVFLGTNDAVKAVAFTPDHRLVSGAENNIARIWRLD
                     DIESPPPGPAMRQWLATHTNLEEETPDDTP"
     gene            complement(2527..2865)
                     /locus_tag="VFV99_08395"
     CDS             complement(2527..2865)
                     /locus_tag="VFV99_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059364.1"
                     /translation="MVWTGVEQSALNTRPGPLICPDTDLNDQAKGSFGQNITYFQPCY
                     PQFKNPALERRQAIATEQKTLKREMQPVPTVTELMEEHPGHAEAVVARAQEEKATSKT
                     GRPPAQTARR"
     gene            complement(2988..3770)
                     /locus_tag="VFV99_08400"
     CDS             complement(2988..3770)
                     /locus_tag="VFV99_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX5059365.1"
                     /translation="MPKWLVTTARLLAVIGVVAIASVAGLIAFRNIVDLDSISESGDS
                     IGSYLQVLGGIYAVLLAFVVIVVWGQFNDARGFVHREANALTDLYRTASGLPPEARDT
                     IQQGLRDYVAAVIADEWQAMTCGDHETIDRVSRRLDRVWSAIHTCMPLNECQHTVYAE
                     VLARFNDLSETRTNRLSAATAKIPTAMNVLLYIGAFIMIGSMYLMPFQKFWLHATVTA
                     ALAGAVAHILFLIYDLDTAFAGDYQVDKGPFERARQTFERNR"
     gene            complement(3804..5666)
                     /locus_tag="VFV99_08405"
     CDS             complement(3804..5666)
                     /locus_tag="VFV99_08405"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX5059366.1"
                     /translation="MDTSSPRAPFVERLSTGALLLDGAMGSVLYDRGVLHTRSYDELN
                     LSQPDLITRIHDEYVTAGADLIETNTFAANRIALTKHGLADRVVDINRAGVELARKAT
                     HGRAYVAGAVGPTGVAFRVAGARERKLARYALAEQIEALVLADVDALILETFASIVEI
                     EEAIRVAKERAAKVPILAMMVFDVAGHGDGDLSPAEIADRLIASGADAVGANCSAGPA
                     ELYQVTTAMVGRGRPVIAQPNAGNPSVVDGRTLYVANAETFGVYTRRLLKGGVGIVGG
                     CCGTTPQHTRAMYGAIRMLRGEDASVQQVDSAESVRPEPAVALAERSRLGRRIAEQRF
                     AVSVELTSPAGLDLTKTKAAVSELLTGGVGTINIADGPRASARMSNLAVCARLAAETA
                     AEPILHVCARDRSLLGLVAHLLGAHALGIRNLVIITGDPPKMGDYPNSSPVYDVDSIG
                     LLRIAAGLNAGVDPAGKQCEPTSFVLATGAEPGARDRERELRRLEQKQRAGAELVMTQ
                     PVYDPRTLESFLADAAPLGLPIMVGILPLASHRNAEFLHNEVPGMSIPAEYRERMAKA
                     GAGPAAREEGIKIAREALNAVSDRVAGVYVMPPFNRVDAALAVLDVVRDRLAAR"
     gene            5772..7049
                     /gene="metK"
                     /locus_tag="VFV99_08410"
     CDS             5772..7049
                     /gene="metK"
                     /locus_tag="VFV99_08410"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5059367.1"
                     /translation="MPSYLFSSESVTEGHPDKLCDAVSDAVLDACLEQDPNSRVACES
                     VTKTGFVLLAGEITTKAVLDYPTIVRQTVHEIGFTSSDMGFDADSCAVMVCVAQQSPD
                     IAQGVDRGTDPAKQGAGDQGLMFGYACDETKELMPMPIQLAHQLAHRLAEVRKKKVKG
                     IDWLRPDGKTQVSVRYEDGQITGVEAIVVSTQHDDKVKQKTIEEAIREHVIKPVVPAK
                     LVTNKTKIHINPTGRFVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDR
                     SAAYYARYIAKHVVGAKLAKKCEVQFAYAIGVAEPVSMLVDTFGTGVVPDEKLTKAVL
                     ETFDARPGMLIQELKLRRPIFRATAAYGHFGRELQNFLWEATPRVDELSDRARGQRAR
                     TSKPPSQARSNKAQRTAAAVAVSPGRGRGPVQL"
     gene            7144..>7782
                     /locus_tag="VFV99_08415"
     CDS             7144..>7782
                     /locus_tag="VFV99_08415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5059368.1"
                     /translation="MSSKPVLDHVLREEADFANQDYAPYANKLEIDPKMFRRYQLPLD
                     LADWRQMSALLLGDVRGKLLLDFGCGMGEESIYFAKLGAKVTSIDISEVGIASLKKRA
                     AYNHLDVRAYEMRADPTSFDDNSFELVHGLGILHHVGIEKGLAEVHRILRPGGIGVFL
                     EPMGDSRVIESVKTWLMTHARFLGNFDHVTDHEHNLTWNEVHSATRQFAEHTL"
BASE COUNT         1403 a         2564 c         2555 g         1260 t
ORIGIN      
        1 tcgcgcgacc gtcgcgtcgt cgcctgccac ggcaggattc gcagagaccc agcctgcacg
       61 ggacgtgacg gcgacggacc cggcgcgtgc gaagcagcgg ctcgcgacgg atccgacgat
      121 gcaaagcgat ccgacgatcg atggtccgat ggccgcgacg ctgcccggac cccatggcgt
      181 cgctgatctg ccgacgctgc agcgcatcga cgattcggcc tatgcgatcg gcgaagagct
      241 cgcgcgcggc ggcatgggca agatcctgtt cgcgcgtgac cgccggctgc gccgcgacgt
      301 cgtgatcaag gtgatgcggc gcgagtcggg ccgcgtcgat ccgcggttcg agcgcgaagc
      361 gttgatcacg gcacgtctgc agcacccctc gatcgtgcgc gtctacgacg ccggcgtgtt
      421 cggcgatggt cgcgcgttct acgcgatgga acgggtgcgc ggccgttcgc tcgatgtcgt
      481 catcgccgac gcgaagaccg tgcgcgaccg actcgcgctc ctgccgcacg ccatcgcagt
      541 ctgcgatgcg atcgcatatg cgcatagcga aggcgtgctg caccgcgatc tgaagccgca
      601 caacgtgctg gtcggtccgt tcggcgagac cgtcgtgatc gattggggcc tcgcgaagga
      661 tttgcgaacg agccaggcag agagcgccag cgaggagcgt cctgccgacc ccgatcgcgg
      721 ctcttcgggc gacatgctca ccgtcgtcgg agccgtactc ggcacgccgt cgtacatggc
      781 acccgagcaa gcacgtggcg aagcgagcga cgagcgcacc gacatctacg cgctcggcgc
      841 gctgctctac acgtcgctga gcggcgagcc accgcatcgc ggtcgcaccg ccgacgaggt
      901 gatcgagctc gtctccggtg gcaaacgcgt cccgctcggc aagcgcgagc ccggcgtgcc
      961 acccgagctg gccgcgatcg tcgagcgcgc gatggcgcac gagccacagg cacgatttgc
     1021 gagcgcactg gagctcgccg aagagctgcg gcgatttgct gccggcaagc tcgtcgcatc
     1081 gcacacgtat tcgctcggtc agctcgtccg gcgctggatc cgtcgctatc gcgtcacgct
     1141 ggcggtagcg gcggtcgcgc tcgtgatcct cgcggtcggt ggtgtgctcg ccgtcaacaa
     1201 cgtgatgacg gagcgcgact tcgcgaacac cgagcgactc aaggccgaag cggccgagga
     1261 tcgcgcgaac acgaagtacg acagcttgct ctatgggctc gcgaagaaag cgctcagcac
     1321 ggacccgagt cacgccgcgg cgtggctcaa gctgatgagt gatcgcggcc tatcgtggga
     1381 caagacccag aaggtcgcga tcgatgcagc gcggctcggc ctcgcgcgcg agctacgcgg
     1441 gcatgcgcag gacatcgagc atgtcgctgt cacgcccgac ggcaagtacg tgatcaccgg
     1501 cagcgacgac tcgacggcgc ggatctggaa cctcgccaac gacgcactcg ttgccgagct
     1561 caagggccat gacgggccga tcgagacgat gacgctgtcg agcgacggga aataccttgc
     1621 gacagcgggc accgacggcg acgtgcggct gtgggaaatc ccgtcgggaa ccggccgtcg
     1681 ccttcagggc caccacaaca cagtgcgcgg cgtgtcgttc tcgcccgaca acacgaaact
     1741 cgcatcgacc ggcgaagacg gcacgctgtt tatctgggac gtgacgagcg cggcgagcaa
     1801 ggagctactc aagcacaccg ccggcctgcg tcccgtgatc tggtacgaca acacgaccgt
     1861 gatcgtcggc ggcttcgacg gcaagatggg tcgctacgac gtcacgacgg gcaagggcag
     1921 catggtgcag gcacacgacg cggagctgcg ctgcctcgcg ctgtcaccgg atcgccgcta
     1981 tctgatctcg ggcgacgaga acggcctcgt cgcattgtgg acgggcgacg gcaagcgcct
     2041 gcgctcgctc ggacgacata ccgacgtgac gcgcgacgtg atgttcacgc ccgacggaaa
     2101 attcgcgctg tcagccggcg gcgatccgat cgtgcgcgta tatccgattc ccgatggcaa
     2161 agcatacgag ctcaccggac acgagaacgg catcaagggt ctcgcgctcg atcgcgatgg
     2221 caagctggtc gcgtcggcgg gcctcgacaa cacggtccgc gtctggacga tcgacggcaa
     2281 accgttccgc gtgttcctcg gtacaaacga cgccgtgaag gccgtcgcgt tcacgccgga
     2341 tcatcggctc gtgtcgggcg cggagaacaa catcgcgcgc atttggcggc tcgatgacat
     2401 cgaatcaccg ccgcctggac cagcaatgcg ccagtggctc gcgacgcaca caaacctcga
     2461 agaagaaacg cctgacgata cgccgtgacg gtcagcggcc aaccgtccga cgatccgacg
     2521 tgacggtcag cggcgagctg tctgcgcagg cggccggccg gtcttgctcg tcgccttctc
     2581 ctcctgcgca cgcgcgacca cggcttccgc gtggcccggg tgctcttcca tgagctcggt
     2641 caccgtcggc acaggctgca tttcccgctt cagggtcttc tgctcggtgg cgatcgcctg
     2701 ccggcgctcg agcgccggat tcttgaactg cgggtagcac ggctgaaagt acgtgatgtt
     2761 ctgcccgaag ctgcccttcg cctgatcgtt caggtcggta tccgggcaga tcagcggacc
     2821 ggggcgcgtg ttgagagcgc tctgctcgac gcccgtccat accatctgtc gaagaccggc
     2881 caccgtaggc tgaggcctct cgccggccgg ctgacatccg ataagcgcga tcaagagaat
     2941 gccgaagcgc atgcaccttt tgaggtgcac gcactgcacc aaggcgctca gcgattacgc
     3001 tcgaacgttt gacgcgctcg ctcgaacggg cctttatcca cctggtagtc accggcgaat
     3061 gccgtatcga ggtcgtagat caggaacagg atgtgagcga cggcgccagc cagtgctgcg
     3121 gtcacggtag cgtgcagcca gaacttctgg aacggcatga gatacatcga gccgatcatg
     3181 atgaacgcgc cgatgtagag cagtacgttc atcgccgtcg ggattttggc cgtcgctgcc
     3241 gagagacgat tcgtgcgggt ctcgctgaga tcattgaatc gcgccagaac ctcggcgtag
     3301 acggtgtgct ggcactcgtt gagcggcatg cacgtgtgga tcgcgctcca cacgcgatcg
     3361 agacgccgac tgacgcggtc gatggtttcg tgatcgccgc atgtcatcgc ttgccactcg
     3421 tcggcgatca cagccgcgac atagtcgcgt aggccctgct ggatcgtgtc gcgcgcctcg
     3481 ggtggcagtc cggacgccgt acgatacaga tcggtcaacg cgttcgcttc gcgatgcacg
     3541 aagccgcgcg cgtcgttgaa ctgtccccac acgacgatca cgacgaacgc gagcaacacg
     3601 gcgtagatgc cgccgagcac ctgcaggtag gagccgatcg aatcaccgga ctcgctgatc
     3661 gagtcgagat cgacgatgtt gcggaacgcg atcaagcccg caacgctcgc gatcgcgacc
     3721 accccgatga ccgcgagcag gcgtgccgtc gtcacgagcc atttcggcat cagccccgat
     3781 tatcacgcgg ggatcgtgac gtgtcacctc gccgcgagcc gatcgcgcac gacgtcgagc
     3841 actgcgaggg ccgcgtcgac gcggttgaac ggcggcatga cgtacacgcc ggcgacgcga
     3901 tcgctgaccg cgttcaatgc ttctcgcgcg atcttgatgc cttcttcgcg cgccgcgggc
     3961 cccgcgccgg ccttcgccat gcgctctcgg tattctgccg ggatgctcat gcccgggact
     4021 tcgttgtgga ggaattcggc gttgcggtgc gacgcgagcg gcagaatccc gaccatgatc
     4081 ggcagcccga gcggcgcggc gtcggcaagg aacgactcga gcgtgcgcgg atcgtaaacc
     4141 ggttgcgtca tcaccagctc ggcgcctgca cgctgcttct gttcgagccg gcgcagctcg
     4201 cgctcgcgat cgcgggctcc gggctccgcg ccggtcgcga gcacgaagct cgtcggctcg
     4261 cactgcttgc cagccggatc gacaccggcg ttgagcccgg ccgcgatccg caacaggccg
     4321 atcgagtcga cgtcgtagac cgggctcgaa ttcggatagt cgcccatctt cggcggatcg
     4381 ccggtgatga tgacgaggtt gcggatgccg agcgcgtgcg cgccgagcag atgggcgacc
     4441 aagccgagca agctgcggtc gcgagcacac acgtggagga tcggttcggc agcggtctcc
     4501 gcggcgagcc gcgcgcacac cgcgaggtta ctcatgcgcg ccgatgcacg cggcccgtcg
     4561 gcgatattga tggtgccaac gcctcccgtg agcagctcgc tcaccgcagc cttcgtcttc
     4621 gtcagatcga gaccggcggg ggatgtgagc tcgaccgaca ccgcgaagcg ttgctcggcg
     4681 atgcgacgtc cgagccggct gcgctccgca agcgcgaccg ccggttccgg ccgtacactc
     4741 tcggcgctgt cgacctgctg cacggacgcg tcctcgccgc gtagcatgcg gatcgcgccg
     4801 tacatcgcgc gcgtgtgctg cggcgtcgtg ccgcaacaac cgccaacgat gccgacgcca
     4861 cctttgagca gccggcgggt gtacacgccg aatgtctcgg cgttcgcgac gtagagcgtg
     4921 cgcccgtcga ccaccgacgg attgcctgcg ttgggctgcg cgatcacggg ccggccgcga
     4981 cccaccatcg cggttgtcac ttgatagagc tcggcgggac cggccgagca gttcgcaccg
     5041 acggcatcgg caccgctcgc gatcaatcga tcggcgattt cagcgggtga cagatcgccg
     5101 tcaccgtgcc cggcaacgtc gaacaccatc attgcgagga tcggcacctt cgccgcgcgc
     5161 tccttcgcca cgcggatcgc ctcttcgatc tcgacgatcg acgcgaacgt ctcgaggatc
     5221 agcgcgtcga cgtccgcgag cacgagcgcc tcgatctgtt ccgccagcgc atagcgagcg
     5281 agtttgcgct cgcgtgctcc cgcgacgcga aacgcgacgc ccgtcgggcc gaccgcgcct
     5341 gcaacatagg ctcggccgtg tgtcgccttg cgggcaagct cgacgccggc acgattgatg
     5401 tcgacgacgc gatcggcgag gccgtgcttg gtgagggcaa tccgattcgc cgcaaacgtg
     5461 ttcgtttcga tgagatcagc tcccgccgtg acgtactcgt cgtggattcg cgtgatcaga
     5521 tctggttgcg acagattgag ctcgtcgtag ctgcgcgtgt gcaagacgcc gcgatcgtag
     5581 aggacagaac ccattgcgcc atcgagcagt agtgcgccag tgctcaagcg ctcgacgaac
     5641 ggtgctcgcg ggctgctggt gtccatcgcc cgctaacgcg aagcaaaacg tcggccggca
     5701 ccggcgagcg ccgtgggcac agtgaaaatg gcgcgcactt gcggcagcgc gaccgccgtt
     5761 aatagtcgag catgcccagc tatctgttct cgtccgagtc agtcaccgaa ggtcaccccg
     5821 acaagctgtg cgacgcggtc tccgatgccg tactcgatgc gtgcctcgag caagatccca
     5881 acagccgtgt cgcgtgtgag agcgtgacga agaccggctt cgtgttgctc gccggtgaga
     5941 tcacgacgaa ggcagtgctc gactatccga cgatcgtgcg ccagaccgtg cacgagatcg
     6001 ggttcacgag ctcggatatg ggcttcgatg cggattcgtg tgcggtgatg gtctgcgtcg
     6061 cgcagcagtc acccgacatc gcgcagggcg tcgaccgcgg caccgatccg gcgaaacaag
     6121 gcgcgggcga tcaggggctg atgttcggct acgcgtgcga cgagacgaag gaactgatgc
     6181 cgatgccgat ccagctcgcg catcagcttg cacataggct cgccgaggtc cgcaagaaga
     6241 aggtcaaggg catcgactgg ctccgccccg acggcaagac ccaggtctcc gtccgttacg
     6301 aggacggcca gatcaccggc gtcgaagcga tcgtcgtctc gacccagcat gatgacaagg
     6361 tcaagcaaaa gacgatcgag gaggcgattc gcgagcacgt tatcaagccc gtcgtgccgg
     6421 cgaagctcgt caccaacaag accaagatcc acatcaatcc gacggggcga ttcgtgatcg
     6481 gcggcccgca cggcgatgcc ggcctcaccg gccgcaagat catcgtcgac acctacggcg
     6541 gctatgcacg tcacggtggc ggcgcgttct cgggcaagga tccgagcaag gtcgatcgct
     6601 cggccgcgta ttacgcgcgc tacatcgcga agcacgtcgt cggcgcgaag ctcgcgaaga
     6661 agtgcgaagt gcagttcgcc tacgcgatcg gcgtcgccga gccggtgtcg atgctcgtcg
     6721 acacgttcgg caccggcgtt gtccccgacg agaagctcac gaaggccgtg ctcgagacgt
     6781 tcgacgcgcg ccccggcatg ctgatccagg agctaaagct gcggcgtccg atcttccgcg
     6841 ccaccgccgc gtacggccac ttcggccgcg agctacagaa ttttctgtgg gaagccacgc
     6901 ctcgcgtgga cgagctgagc gatagggcac gcggtcaacg ggcgcgcacc agcaagccgc
     6961 catcgcaagc acgtagcaac aaagcacagc gcactgctgc tgcggttgct gtgagcccag
     7021 ggcgcggccg cggccccgtc caactgtagt ctcagcgcgc ttcgaacgcc tccgccgtta
     7081 tccgagaagc cggcccgcga actgcacagc atcggagcgc cgcttctcac ccacggaggc
     7141 tatgtgagct caaaacccgt tctcgatcat gtgctgcgcg aggaagccga cttcgcgaac
     7201 caggactacg cgccgtacgc caacaagctc gagatcgatc cgaagatgtt ccggcgctac
     7261 cagctcccgt tggatctcgc cgattggcgg cagatgtccg cactcctgct cggcgacgtg
     7321 cgcggcaagc tcctgctcga ttttggctgc ggcatggggg aagagtcgat ctacttcgcc
     7381 aagctcggtg cgaaggtcac gagcatcgac atctccgagg tcggcattgc cagtctgaag
     7441 aagcgcgccg cgtacaacca tctcgacgtt cgcgcctacg agatgcgtgc ggatccgacg
     7501 agcttcgacg acaacagctt cgagctcgtg cacgggctcg ggattctcca tcacgtggga
     7561 atcgagaagg ggctcgccga ggtccaccgc attctgcggc cgggcggcat cggcgtgttc
     7621 ctcgagccga tgggcgacag ccgcgtcatc gaaagcgtga agacgtggct aatgacgcac
     7681 gcgcgcttcc tcggcaactt cgatcacgtc accgaccacg agcacaacct gacgtggaac
     7741 gaggtgcact cggcgacgcg acagttcgcc gagcacacgc tg
//
LOCUS       DASUFX010000214         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_588063,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000214 DASUFX010000000
VERSION     DASUFX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588063"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VFV99_08420"
     CDS             <1..1376
                     /locus_tag="VFV99_08420"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5059369.1"
                     /translation="SNEDVAHAVNAIGNTHLMRGDFDTAEPMYARALALAVNVEDRQL
                     EAMIAQNLGVIASVRGDLTAALDYYRSSLAIYRAQSAHRQIGHTLNNMGLVYTQMECY
                     DDARAAYDEAVVHCRVAGDVPHRLLALNSSVSLLIAQGDIERAATVAGALLHEARDAG
                     DQRALGETFKNLGVICRSRGEIEAAERHLREAFDNALRREDLLLAAETATEQAELFEK
                     MTKNRETLQALSLSHRLFTRLRAERNLADLQRRVARLEDRFYLVVARWARDIESKDAY
                     TRGHCERVADYACALARDVGFDEMTMFWFRIGAVLHDVGKIAVPSEILNKPGRLTAEE
                     RVIMEQHAAAGAELLSSIEFPWDILPMVRSHHERWDGSGYPDRLAGEEIALSARIVCV
                     ADVFDALTTDRPYRRGFTRDEALMMMNAERGKTFDPDLLARFERLIRATRLYNLPAQM
                     PAVAIAS"
     gene            complement(1383..1865)
                     /locus_tag="VFV99_08425"
     CDS             complement(1383..1865)
                     /locus_tag="VFV99_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX5059370.1"
                     /translation="MIEALKQKLGEEVEKLQYELNVTLPNEIRKAVELGDLRENSEYK
                     AALERQQFVQARLGQLRQRLSKLSSIDPTQIPRDKVGLGSKVVVEDQKTKQRETYYLV
                     FGDALEFEEGHVTMASPIGRALLGQGVGDVAFLKLPSMVRQLKVVELKTLHDADEGDL
                     "
     gene            2012..2785
                     /locus_tag="VFV99_08430"
     CDS             2012..2785
                     /locus_tag="VFV99_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5059371.1"
                     /translation="MTRLRMVSAAGRAIGIDVSAYVVRGVMIDTGFAHAREALLRAAR
                     SLSVRAAIVTHWHEDHAGNVSTIAALGVPIALRADTEAVLRSFPPIELYRRVVWGAPL
                     PLGAPIQGTELDGLVCVHTPGHSVDHQIVWDPSTRSAFTGDLWLGVRARTVHSTEDPY
                     TIVESLRTLRDLRPARMFDAHRGLIEPAVEAIDRKIEWLSMTLATIESRIVAGDDDST
                     IVRRVLGGEDLAAVVSRGSYSRRNLVRSVRRRTRLGSIG"
BASE COUNT          466 a          934 c          943 g          523 t
ORIGIN      
        1 gctccaacga ggacgtcgcg cacgccgtga acgcgatcgg caacacgcac ctgatgcgcg
       61 gcgatttcga taccgccgag ccgatgtacg cccgcgcgct ggcgctcgcc gtgaacgtcg
      121 aggaccgcca gctcgaggca atgatcgcgc agaatctggg cgtgatcgcc agcgtccgcg
      181 gtgacctgac cgccgcgctc gactactacc gatcgagcct ggcgatctac cgtgcgcagt
      241 cggcgcaccg ccagatcggc catacgctca acaacatggg cctcgtctac actcagatgg
      301 agtgctacga cgacgcgcgc gccgcatacg acgaagccgt cgtccactgc cgtgtcgcgg
      361 gcgacgtgcc gcaccggctg ctcgcgctca acagctcggt gagcctgctg atcgcgcagg
      421 gcgatatcga gcgcgccgcg acggtcgccg gcgcgctcct tcacgaagca cgcgacgccg
      481 gtgaccagcg ggcgctcggt gagacgttca agaacctcgg cgtgatctgc cggtcgcggg
      541 gtgagatcga agcggccgag cgacatctcc gggaagcatt cgacaacgcg ctccgccgcg
      601 aggatctgtt gctcgccgcc gaaacggcga ccgagcaggc ggagctgttc gagaagatga
      661 cgaagaatcg cgagacgctg caggcgctct cgctgtcaca ccgtctcttc acgcggctgc
      721 gggccgagcg caatctcgcc gacctgcagc gccgcgtcgc ccggttggag gatcgcttct
      781 acctcgtggt cgcgcgctgg gcgcgcgaca tcgagtccaa agacgcctac acgcgcggcc
      841 actgcgagcg cgtggccgat tacgcctgcg cgctggcgcg cgacgtcggc ttcgacgaga
      901 tgacgatgtt ctggttccgc atcggcgccg tgctgcacga cgtcggcaag atcgcggtgc
      961 cgtcagagat cttgaacaag ccgggacgcc tgacggccga agagcgcgtg atcatggagc
     1021 agcacgccgc ggccggcgcc gagctgttgt cgtcgatcga gttcccgtgg gacattctgc
     1081 cgatggtgcg cagccaccac gagcgctggg acggctcggg gtatcccgat cggctcgccg
     1141 gcgaagagat cgcgctcagc gcgcgcatcg tctgcgtcgc cgatgtgttc gacgcgctga
     1201 cgacggatcg cccctaccgc cgcggcttca cccgcgacga agcgctgatg atgatgaacg
     1261 ccgagcgcgg caagacgttc gatcccgacc tgctcgctcg cttcgagcgg ctgattcgcg
     1321 cgacgcggct atacaatctg ccggcgcaga tgccggcggt cgcgatcgcg agctaacgaa
     1381 ccctacagat cgccttcgtc cgcgtcgtgg agcgtcttca gctcgacgac cttcagttgc
     1441 cgcaccatcg acggcagctt gagaaacgcg acgtcgccca caccctgccc gagcagcgct
     1501 cgtccgatcg gcgaggccat ggtgacgtgg ccctcctcga attcgagcgc gtcgccaaaa
     1561 acgagataat acgtttcgcg ctgtttggtt ttctggtcct cgacgacgac cttcgagccc
     1621 agaccgacct tgtctcgcgg gatttgcgtt ggatcgatgg atgagagctt gctcaggcgc
     1681 tgacggagct gtccgagccg cgcctgcacg aactgctgac gctcgagcgc ggctttgtac
     1741 tcgctgttct cgcgaagatc gccgagctcg actgccttcc tgatctcgtt gggcagcgtc
     1801 acgttgagct cgtattggag cttctcgact tcttccccca atttttgctt cagtgcttcg
     1861 atcatacgcg gaccgcgtga gtcataggac aaaacttgag cgcccggccg gaaggccggg
     1921 cgcgttgggc tatatgaagt tactcgccgt gcgaaaccct cgcaaacggt agccgatgct
     1981 ggcttcgctt cgagtcgagc agcatggaga cgtgacgcgg ctgcgcatgg tgagcgccgc
     2041 cggacgcgcg atcggaatcg acgtcagcgc gtacgtcgtg cgcggcgtca tgatcgacac
     2101 gggattcgct cacgcgcggg aagcgctgtt gcgggccgcg cgatcgttga gcgttcgagc
     2161 ggcgatcgtg acgcattggc acgaggatca tgcgggcaat gtctcgacga tcgccgcgct
     2221 cggcgtcccg atcgcgcttc gcgccgacac ggaggccgtg cttcgctcct ttcctcccat
     2281 cgagttgtat cggcgcgtgg tctggggcgc gccgctccca ctcggcgcgc cgattcaagg
     2341 cacggagctc gacggtctgg tttgcgttca caccccgggc cactcggtcg atcatcagat
     2401 cgtctgggat ccatccacgc gatcggcgtt cacgggagat ctgtggctcg gcgtgcgcgc
     2461 acgcaccgtc cattcgacgg aagatccgta caccatcgtt gaaagcctgc gcacgctgcg
     2521 cgaccttcgc cccgctcgca tgttcgacgc tcatcgcgga ttgatcgagc cagcagtcga
     2581 agcaatcgat cgcaagatcg aatggctgtc gatgacgctc gcgacgatcg agtcgcggat
     2641 tgtcgcgggc gacgatgatt ctacgattgt gcgccgagtg ttgggcggag aggatctcgc
     2701 ggccgtcgtg tcgcgcggga gctactcgcg tcgcaatctc gttcggtcgg tgcgtcgccg
     2761 gactcgcctt ggttcgatcg gataggcgac cggctttggc ctttggggcc tttggtgggg
     2821 cctttggggt cagactccgt tcgcggcttc ggggtcagac tccgtt
//
LOCUS       DASUFX010000215        12284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1562289, whole genome shotgun sequence.
ACCESSION   DASUFX010000215 DASUFX010000000
VERSION     DASUFX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562289"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="VFV99_08435"
     CDS             <1..1801
                     /locus_tag="VFV99_08435"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX5059372.1"
                     /translation="MLGVLAALAACPSGGGDGKVAKPVPPPLPGNEGKLDLPEIPAKI
                     DITQSAPTEGNSPAARSPILDILKNENDREIGALKKASEPAYYLAYQLVEQRVVSLEA
                     EGGALISDNDDTARNLDVEVRVGSAKLDNTRAISDDNNNFNAPLTRRGVVPFGEDKQA
                     LTNALWVETDRRYHEAVTALGYVKQDQSTLSHASSDPDFSSAPADVYIEKPAQLTFDK
                     AQWIDRLKRCSAKALKGDATRGTCGVVFQLNTAYYVNSEGAQLQLSWTNAQLSVSVGV
                     KAEDGMNLSRLEQRFGRTPADLPPDTEVNKMIEEVTTDLDKLHDAPLAEPYVGPAILQ
                     GRASGVFFHEVFGHRIEGHRQKDTTSGRTFASYVGKDIAPEWLSVYDNPELVTLNGVQ
                     LNGFYRFDDEGVKAQKVPLVDKGKLVGFLMSRNPIPGFPNSNGHGRHSPGLPPVARQG
                     NLVVETTRSVPYADLEKMLIEEIKKQKRPYGMIFTDISGGFTNTSAFAPQAFKVNPVM
                     AYRIYPDGKKELVRGIDISGTPLVALQSIRAASREVETFNGVCGAESGWVPVSASAPS
                     LMIERIEVEKGFIPPDRPPLLPPPSSIKQEGSR"
     gene            1798..3639
                     /locus_tag="VFV99_08440"
     CDS             1798..3639
                     /locus_tag="VFV99_08440"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX5059373.1"
                     /translation="MKTRYLAVALALLPATTSFAKKPPEPMGSPSKAVTDQIVDAIAE
                     EMNRAMTKLEIPGAPRPYQISYKITEVDVNDVSATLGQTTSRRNRHFVNLEARVRVGS
                     NALDNSNFVVPDGSEFDGTAAANLPLDSDERIARRAAWLVTDTAYKEALIQLRAKLEA
                     RRAGASSRPADVPAWTDTKKVVSEEPVLVAPLETLDELETRVKSLSAVFRDQPALRDS
                     RVAATSYLERRWYLTTEGTSVTDTRRASGIVIAVSGQAEDGQLLGDYFLRYGHTAKDL
                     PSDKELTEEAKKLAQSVTALVKAPKIDKYSGPVLFEGEGAVGLVRFALAPHLGGTPIP
                     EGMSPQEARLVGGALIDKVGLRVTSPNLSIIDDPTAKEGAGKALIGGYKTDDEGVAAQ
                     KVEVVKNGTLKTLLLSRTPSAKGQASNGHARCCSVSSPSDGGAFHGSATNLFVTAAGG
                     QPRKALEARVAQLAKGEGLKYGLIIKRFDDAAVTGAPEFTRRELVQMIKSTDPLLPPP
                     TLVAYRVYPNGKQELVRGAQLTDVPIRAWKDVLGTSKEVTVYNFLNATDSQLAQRLSG
                     GTDDGFVPSGGIESSIITPDLLLKEIDVTTSTYGVRPTPSVPKLTAK"
     gene            3636..4193
                     /locus_tag="VFV99_08445"
     CDS             3636..4193
                     /locus_tag="VFV99_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HEX5059374.1"
                     /translation="MNLRVHAVEPRSRANGPGARFVVWLQGCTLGCPGCFNPTTHDPS
                     SGSETTVAELAAQLGATRDIEGLSLSGGEPLQQAEAAAALLDAARALGLSTLVFSGYT
                     IDEIRALPHGPAVLDRLDVLIDGRYVAGERLATGLRGSANQRIHVLTSRYQRADVEAT
                     PVAEIRIAPGGEVVLTGVNPLKLKR"
     gene            4228..4452
                     /locus_tag="VFV99_08450"
     CDS             4228..4452
                     /locus_tag="VFV99_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059375.1"
                     /translation="MSDQFDLRDTRGRLKKLALAALIGAVLTFFTVRAMMSSGHGNHD
                     PVGASIMPLLGVFIFVLTTAFAHRIVSKKR"
     gene            4488..5168
                     /locus_tag="VFV99_08455"
     CDS             4488..5168
                     /locus_tag="VFV99_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059376.1"
                     /translation="MPRLAVPRGLDRRALRCRREFLGHFPGGYRDDTYLAWERDYKVE
                     AHEAWERELSQDKLDELIDAREFDTIAQHAVRIESRTNLLFSFEKMALRDALRSPVGA
                     RTFALGINELLHGKGSLADRFENWVDAVSQLPRKQTRVLTWPVVTVFGMIGAPREHIF
                     LKPWTTQIAAEAYGFDFLYESRPNWMTYKSLLDFAARVRRDTRDLKPRDMIDLQSFIW
                     VLGSYEYD"
     gene            complement(5165..5869)
                     /locus_tag="VFV99_08460"
     CDS             complement(5165..5869)
                     /locus_tag="VFV99_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5059377.1"
                     /translation="MKKCPFCAEEIQDEAIKCRFCGSFLSAAPPAGTATPPAGTPTAK
                     PAAPAPEPGTGAPPFARAAGTSKDDPKGRKMLYEGHPSWKAYLGHYMLAGFIAIAIMA
                     IKSAIDGSGAPLMTKVLNVVVPLAMAAVYLFGLTLFRHSIKFRVSNTAVEYERGLFSK
                     RIDVMQLWRVRDVTYKQNLVDRILGIAHIDLVSSDVSTPHLEIVGLPASRQLFENLRD
                     SIEIQRQAKNVYGVVS"
     gene            complement(5872..7008)
                     /locus_tag="VFV99_08465"
     CDS             complement(5872..7008)
                     /locus_tag="VFV99_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059378.1"
                     /translation="MKKSLVFAALLVPALALAQPKSSDDWYKEGETQYNLGEFDKAAD
                     AFKQGFALETNDSKKPAYLYNVAQAYRQGGKCKDAAFFYKRYLSLKDQDTVKPLKPEK
                     RAEIEQKITELEECARTAESIAKKPPDNTLPNEGGNKTGTGTGAGTKTGTGTGTGTGT
                     GTKTGPTVGSGGGEGEGEGEGEGEGEGEGDGGGGAVTGQFMVKPRTLNARFVGGVGKV
                     SMGDVPTGIKPAITITGGYPVVTQGSLSVDVGVSLNFTPMPYENGLTGAKETGTMTTA
                     VANAAATINVAPKIAVRGDLGLGALFFGGLKMGNPFTEGAAATSGSLGMFALRLGGSL
                     DYGLTPNVSATVTPLALTYSPAKDGLRSGSIIQLDFMVGVGYRM"
     gene            complement(7046..8704)
                     /locus_tag="VFV99_08470"
     CDS             complement(7046..8704)
                     /locus_tag="VFV99_08470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059379.1"
                     /translation="MGSVYLAEHPTIGKKVALKILHAEFASNPEVADRFFTEAKAVNA
                     IGHPNIVDIVDYGVIQSGPGGRDRLVYFIMEYLAGHTLSQAIRTEAPIPPERALMIAM
                     QVADALAASHKNSIVHRDLKPDNIILIQRGRERDFVKLLDFGIAKLTGSDGLSSHKTR
                     TGLVLGTPAYMSPEQCEGRATVDHRTDIYALGVVLYEMLVGRVPFIGEGYGEILVQHL
                     TQQPIPPSQYRMMSPHVEQVVLKALEKRPDMRFPTMDEFMRGMADPVGYVESHGGLTG
                     FMQRQLMPSSAPLPPMRLTPAPMTPVPGTPTGMPMTGVNMRSPVPGTLTTPTTLSAAA
                     GQVQPQKSKTPFVIAAALVVVLAAGGVFMLLNKKKGTESAAKPDEGSQVVDKGSQVQP
                     PPQPPQPPPQPPQPPVENKGSAAAGSAMTVENKGSAENKGSAAVPTNKGSAEQKDVVP
                     TIAAVTITSNPPGAEILINGAPIGKKTPQTLSLPIGKDVTVLLKLSGFADYKKSVAIK
                     NDKINFDAALRKVSSGGGKGSGAGKGSGKGSGQKQCDTCLERPD"
     gene            8678..9772
                     /locus_tag="VFV99_08475"
     CDS             8678..9772
                     /locus_tag="VFV99_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5059380.1"
                     /translation="MLGQVDRPHAAFPQLLGDQIVADGLTDDVIWRGRPSLWVHFHSA
                     SHHTSSQATEKSRLRVRRVNSIMMRPFVLWVIVGVLGRSAFANDLPDVKSRSAVVIDS
                     ETGAEIFGKDADEIRAIASTTKIFVAMAVRKKGMDLDGWTEITRADVQHARGGARTRL
                     DIRRVFRNKDLLRAMLMASDNRAPTALGRAAGMGPDELVAAMNDIAKDLHLKRTKFTD
                     PTGLRGNVSTAREMALALRAALEDDVLREIMGDPFELIVGKGGRPKIAYGSTNQALVV
                     HKYDVIGGKTGYTTAAGYCFVTGARFNGREVVMTFLGAPGKSSRFGDFNRVAAWLESG
                     APGAKVNVAAIAKRPKLKIEGDVRGRVAKK"
     gene            9788..11224
                     /locus_tag="VFV99_08480"
     CDS             9788..11224
                     /locus_tag="VFV99_08480"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX5059381.1"
                     /translation="MTWSRREVLASLGVASAQAALWACGAPAQRVVRRATEVNPELRT
                     WLHAAVATLKGAGFASAHVLATSRQRTTAAIDVLGAGVARARCDGLVITVRDRDGMLR
                     EQVTNDLSQAGIAAAVTVLAGSAKPASVDFGKTPAAFSRFRADPDVLADSDILARVAA
                     LAKRDRESLSSRIVYSAAVMDIDDARVWSVAPGHDMQQRLVHVKKSVTRVAWNGTRPV
                     VSEVSRAWAGGLDEQDLDDDELVAAREGALALMTPRAFEDHEYAFALEPAVAAAVIDA
                     AVQSLFTPSAARRPEVAAVLPIGAKVMSPIITVVDDPGAARAYGGFVFDDAGEPAAAR
                     TLIERGTIAARLDRIRRPGHVGRSDPYASHLRVTPGAIEQNDLLDDGFLLEGPLGAVV
                     DPTSDRLVISVARARERVAGKRTGRMFAEIELVGELSKIMGSVDGLSKQTKTIGVRDE
                     VDGQPRWRSIEAPWVRGKALLRARRRAV"
     gene            11176..>12284
                     /locus_tag="VFV99_08485"
     CDS             11176..>12284
                     /locus_tag="VFV99_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059382.1"
                     /translation="MGAWQSAAACAQESGVITRRELASTLARRGLADWVVVERTQDLA
                     SIDERTKVKRVERRVVWCVTVHFDSPQGRGSAHVTVDAVDGAASAAVEQAVALAQSSI
                     GPAWISRPLAAPARVKLEDTSLAKQAPVDIAADVLKKLARPTAVAARATVLREHVSVV
                     ARQGFHTEWTATLLSCDALVAANQRSLLIARGARQRSALELDTAVTAAQQDLDLVAKA
                     GAPTAGPCALVLATDAMLHDGLGVWATFANQADAVVERQGLTRYREHTPIVPGAEQVS
                     EPLTITSDGASDYGLLSAPLGDYGDAVRTFPLVNRGVAVGLGLTPREGSLRGRDPNGG
                     VRNLVVEPGGWSGEIDGSQPVRMVEVRRLRSLSIDP"
BASE COUNT         2089 a         4053 c         4065 g         2077 t
ORIGIN      
        1 catgctcggc gtgctcgccg cgctagcggc ctgcccgagt ggcggtggtg acggcaaagt
       61 cgccaagcct gttcccccgc ccctgcctgg caatgaaggc aagctggatc tccccgagat
      121 cccggcgaag atcgacatca cccagagtgc gccgacagag ggcaactcgc ccgcagcgcg
      181 ctcgccgatc ctcgacatcc tcaagaacga aaacgatcgc gagatcggtg cgctcaagaa
      241 ggcatccgag cctgcgtact acctcgcata ccagctcgtc gagcaacgcg tggtcagcct
      301 cgaagccgag ggtggcgcgc tgatctccga caacgacgac accgcgcgta acctcgacgt
      361 cgaggtccgc gtcggttcgg caaagctcga caacacgcgt gcaatctccg acgacaacaa
      421 caacttcaac gcgccactca cccgtcgcgg cgtcgtgccg ttcggcgagg acaagcaggc
      481 gctgacgaat gcgctgtggg tcgagaccga tcgccgctat cacgaagcgg tcacggcgct
      541 cggctacgtc aagcaggacc aatcgaccct gtctcacgcg agctcggatc cggatttctc
      601 gtcggcgccc gccgatgtct atatcgagaa gccggcgcag ctgacgttcg acaaggcgca
      661 gtggatcgac cggctcaagc gctgttcggc gaaggcgctc aagggcgacg cgacgcgtgg
      721 cacctgcggc gtcgtgttcc agctgaatac cgcgtactac gtaaacagcg agggcgcgca
      781 gctccagctg tcgtggacaa acgcgcagct ctcggtgtcg gtcggcgtca aagccgagga
      841 cggcatgaac ctgtcgcggc tcgagcagag gtttggccgc acgcccgcag acctgccgcc
      901 cgataccgag gtcaacaaga tgatcgagga ggtcacgacc gacctcgaca agctgcacga
      961 cgcaccgctc gccgagccct atgtcggtcc cgcgatcctg cagggccgcg cgtccggcgt
     1021 cttcttccac gaggtgttcg gccatcgcat cgagggccat cgccagaagg acacgacgag
     1081 cggccgcacg ttcgcgagct acgtcggcaa ggacatcgcg ccggagtggc tgagcgtcta
     1141 cgacaacccc gagctcgtca cgctcaacgg cgtccagctc aacggcttct atcgcttcga
     1201 tgatgaaggc gtcaaagcgc agaaggtgcc gctcgtcgac aagggcaagc tcgtcggctt
     1261 cttgatgagc cgcaacccga ttcccggctt cccgaattcg aacggccatg gccgtcactc
     1321 accgggcctg ccgccggtcg cgcgccaggg caacctcgtc gtcgagacca cgcgcagcgt
     1381 gccgtacgcc gacctggaga agatgctgat cgaagagatc aagaagcaga agcggccgta
     1441 cggcatgatc ttcaccgaca tcagcggtgg cttcacgaac accagcgcgt tcgcgccgca
     1501 ggcgttcaag gtcaatccgg tgatggcgta tcgcatctac cccgacggca agaaggagct
     1561 cgtccgcggc atcgacatca gcggcacgcc gctcgtcgcg ctgcagtcga ttcgcgccgc
     1621 gagccgcgag gtcgagacgt tcaacggcgt gtgtggcgca gagagcggct gggtgccggt
     1681 ctcggcgtcg gcgccgagct tgatgatcga gcgtatcgag gtcgagaagg gcttcattcc
     1741 gcctgaccgg ccgccgctgt tgccgccgcc atcgtccatc aagcaggagg gttcgcgatg
     1801 aaaactcgtt atcttgccgt cgcgctcgcc ctgctccccg ccacgacgtc gttcgcgaag
     1861 aagccgcccg agccaatggg ctcgccgtcg aaggcggtca ccgatcagat cgtcgacgcg
     1921 atcgccgaag agatgaatcg cgcgatgacg aagctcgaga ttccgggcgc gccgcgtccg
     1981 taccagatct cgtacaagat caccgaagtc gacgtgaacg acgtctcggc gacgctcggc
     2041 cagacgacgt cgcggcgtaa ccggcacttc gtcaatctcg aagcgcgcgt gcgcgtcggc
     2101 tcgaacgcgc tcgacaactc gaacttcgtc gttccggacg gcagcgaatt cgacggcact
     2161 gcagccgcga acctgccgct cgattccgac gagcgcatcg cacgccgcgc agcgtggctc
     2221 gtcaccgaca ctgcatacaa agaggcgctg atccagctgc gcgcaaagct cgaagcccgc
     2281 cgtgcaggcg cctcctcacg gcccgccgac gtgccggcat ggaccgacac gaagaaggtc
     2341 gtcagcgaag agcccgtgct cgtcgcgccg ctcgagacgc tcgacgagct cgagacacgc
     2401 gtgaagtcgc tgtcggcggt gttccgcgat caacccgcgc tgcgtgactc gcgcgtcgcc
     2461 gcgacgagct acctcgagcg ccgctggtac ctgaccaccg agggcacgag cgtgaccgac
     2521 acgcggcgtg cgagcggcat cgtgattgcg gtgagcggcc aagccgagga cggccagctg
     2581 ctcggcgatt acttcctgcg ctacggccac accgcaaagg atctgccgag cgacaaggag
     2641 ctcaccgagg aagcgaagaa gctcgcgcag tcggtcacgg cgctcgtgaa agcgccgaag
     2701 atcgacaagt acagtgggcc cgtgttgttc gaaggcgaag gtgccgttgg cctcgtccgc
     2761 ttcgcactcg caccgcacct cggcggcaca ccaattcccg aagggatgtc gccgcaagag
     2821 gcgcggctcg tcggcggcgc gctgatcgac aaggtcggcc tccgcgtgac gtcgccgaat
     2881 ctatcgatca tcgacgatcc aaccgcgaag gaaggcgctg gcaaggcact gatcggcggc
     2941 tacaagaccg acgacgaagg cgtcgccgcg cagaaggtcg aggtcgtcaa gaacggcacg
     3001 ctgaagacac tcttgctctc gcgcactccg tccgcgaagg gccaagcatc gaacggccac
     3061 gcgcggtgct gctcggtgag cagcccatcc gacggtggcg cattccacgg cagcgcgacg
     3121 aacctattcg tcacggcggc cggcggacag ccgcgcaagg cactcgaagc acgcgtcgcg
     3181 cagctcgcga agggcgaagg cctcaagtat ggcctcatca tcaagcgctt cgacgacgcg
     3241 gccgtgaccg gcgcaccgga gttcacgcgc cgcgagctcg tgcagatgat caagtcgacc
     3301 gatccgctgc tcccgccgcc aacgctcgtg gcgtaccggg tgtacccgaa tggcaagcag
     3361 gagctcgtgc gtggcgcgca gctcaccgac gtgccgatcc gcgcctggaa ggacgtgctc
     3421 ggcacgagca aggaagtgac cgtctacaac ttcctgaacg cgaccgacag ccagctcgcg
     3481 cagcgtctga gcggcggcac cgacgatggt ttcgtgccgt cgggcggtat cgaaagcagc
     3541 atcatcacgc ccgatctcct gctcaaggag atcgatgtca cgacgagcac gtatggcgtg
     3601 cgtccgacgc cgtcggtgcc gaagctgacg gcgaagtgaa cctccgcgtc cacgcggtcg
     3661 agccgcgcag tcgggccaac ggtccaggcg cgcggttcgt cgtgtggctg cagggctgta
     3721 cgctcggctg cccgggctgc ttcaacccga cgacgcacga cccgtccagt ggcagcgaga
     3781 caacggtcgc cgagctcgcg gcgcagctcg gtgccacgcg cgacatcgag ggactctcgc
     3841 tgtcgggcgg cgagccgctg cagcaagccg aagcagccgc cgcactgctc gacgcggcgc
     3901 gtgcgctcgg cctgtcgacg ctcgtgttct cgggctacac gatcgacgag attcgcgcgc
     3961 tgccccacgg ccctgccgta ctcgaccgcc tcgatgtcct catcgacggc cgctacgtcg
     4021 ccggcgagcg gcttgccacc gggctgcgcg gctcggcgaa ccagcgcatt cacgtactga
     4081 cgtcgcgcta ccagcgcgcc gatgtcgaag cgacgcccgt cgccgagatc cggatcgctc
     4141 ccggtggcga ggtcgtgctc accggcgtga atccattgaa gctcaagcgc tgatgcaacc
     4201 gccgccgcgg cgtgctgtct aaccagcatg agcgatcagt tcgatctgcg agatacccgc
     4261 gggcgtctca agaagctcgc gctggcggca ctgatcggcg cggtgctcac attcttcacc
     4321 gtgagggcca tgatgtcgtc gggccacggc aatcacgacc cggtcggcgc gagcatcatg
     4381 ccgctcctcg gcgtgttcat cttcgtgctt acgactgcgt tcgcgcatcg catcgtgtcc
     4441 aagaaacgct gattgcgcga cgacgatgat gtggccacgg tggccgcatg cctcgcctag
     4501 ctgtaccgcg tgggctcgat cgcagagcgc tgcgctgccg ccgcgaattc ctcggtcact
     4561 ttcccggcgg ctatcgcgac gacacgtacc ttgcgtggga gcgcgactac aaagtcgaag
     4621 cgcacgaggc gtgggagcgc gagctgtcgc aggacaagct cgacgagctc atcgacgcga
     4681 gagagttcga cacgatcgcg cagcacgcgg tccgcatcga gtcgcgcacg aatctgttgt
     4741 tctcgttcga gaagatggcg ctgcgcgacg cgctgcgatc accggtcggc gcacgcacgt
     4801 tcgcgctcgg catcaacgag cttttgcacg gcaaaggttc gctcgccgat cggttcgaga
     4861 actgggtcga cgccgtctcg cagctgccgc gcaagcagac gcgcgtgctg acgtggccgg
     4921 tcgtgaccgt gttcggcatg atcggcgcgc cccgcgagca catctttctc aagccatgga
     4981 cgacgcagat cgcagcagaa gcgtacggct tcgacttcct ctacgagtcg cggccgaact
     5041 ggatgacata caagagtctg ctcgacttcg ccgcgcgcgt gcgcagggat acacgcgacc
     5101 tgaagccgcg cgacatgatc gacctccagt cgttcatctg ggtgctcggc agctacgagt
     5161 acgactagct gacgacgcca tagacgttct tcgcctggcg ctggatctcg atcgagtcgc
     5221 gcaggttctc gaacagctgg cgcgatgcgg gcaagccgac gatctcgaga tgcggcgtcg
     5281 atacgtcgct cgatacgaga tcgatatgcg cgatgccgag gatgcgatcg acgaggttct
     5341 gcttgtaggt gacgtcgcgc acgcgccaga gttgcatcac gtcgatgcgc ttgctgaaca
     5401 ggccgcgctc gtactcgacc gcggtgttgg acacgcggaa tttgatcgag tgccggaaca
     5461 gcgtgagacc gaacaagtag accgccgcca tcgcgagcgg cacgacgacg ttcagtacct
     5521 tcgtcatcag cggcgcaccc gacccatcga tcgcgctctt gatcgccatg atcgcgattg
     5581 cgatgaaccc agcgagcatg tagtggccga ggtaggcctt ccacgacgga tgcccttcgt
     5641 agagcatctt gcggcccttc ggatcgtcct ttgacgttcc ggccgcgcgc gcgaacggcg
     5701 gcgcacccgt gcccggctca ggagctggtg cagcaggctt cgcagtcggc gtgccggccg
     5761 gcggcgtcgc ggtgccggca ggaggtgcgg cgctcaagaa ggagccacag aaccggcact
     5821 tgatcgcctc gtcctgaatc tcctccgcgc aaaacggaca tttcttcatt gctacatccg
     5881 gtaaccaaca ccgaccatga agtcgagctg gatgatggag ccggagcgca ggccgtcctt
     5941 cgccgggctg tacgtcagcg cgagcggcgt gaccgtggca gagacgttcg gcgtcagccc
     6001 gtaatcgagc gacccaccga ggcgcaacgc gaacatgccg agcgagcctg acgtagcagc
     6061 cgcgccctcg gtgaacggat tgcccatctt caggccgccg aagaacagtg cgccgaggcc
     6121 aaggtcgcca cgcaccgcga tctttggcgc cacattgatc gtcgcggccg cattcgcgac
     6181 agcggtcgtc atcgtgccgg tttcctttgc gccggtcagg ccgttctcgt acggcatcgg
     6241 cgtgaagttg agcgacacgc cgacgtcgac gctgagtgag ccctgcgtga cgaccggata
     6301 accgccggtg atcgtgatgg cgggcttgat gccggtcggc acgtcgccca tcgacacctt
     6361 gccgacgccg ccgacgaacc gcgcgttgag cgtgcgcggc ttgaccatga actgtccggt
     6421 gacggcgcca ccgccgccat cgccttcacc ctcgccttcg ccttcacctt cgccttcgcc
     6481 ttccccttcg ccaccaccgc tgccgacggt cggacccgtc ttcgttccgg tgccggtgcc
     6541 ggtgcccgtg ccggtgcccg tcttcgtccc cgcgccggta ccggtgccgg tcttgttgcc
     6601 gccttcattc ggcagcgtgt tgtcgggcgg cttcttcgcg atcgattccg cggtacgtgc
     6661 gcattcctcg agctcggtga tcttctgctc gatctcggcg cgcttctccg gcttgagcgg
     6721 cttcaccgtg tcctggtcct tcagcgacag gtagcgcttg tagaagaacg cggcgtcctt
     6781 gcacttgccg ccttggcgat acgcctgcgc gacgttgtag aggtacgccg gcttcttcga
     6841 gtcgttggtc tcgagagcga agccctgctt gaaggcatca gcggccttgt cgaactcgcc
     6901 gaggttgtac tgggtctcgc cttccttgta ccaatcgtcg gaggacttcg gttgcgccag
     6961 cgccaacgcg ggcaccagca acgctgcgaa cactaggctc ttcttcattg ccgagctcca
     7021 tgcgaccgcg cggggtcgca gtaaatcagt ccggtctctc gaggcaggta tcgcactgct
     7081 tctgaccgct ccccttgccg ctacccttgc cagcgccgct gcccttgccg ccaccactgg
     7141 agaccttgcg caacgctgca tcgaagttga tcttgtcgtt tttgatcgcg accgacttct
     7201 tgtagtcggc gaagccactc agcttcagca gcacggtgac gtctttgccg atcggcaggc
     7261 tcaacgtctg cggtgtcttc ttgccgatcg gtgcgccgtt gatcaggatc tcggcgcccg
     7321 gtggattcga cgtgatcgtc accgccgcaa tcgtcgggac gacgtccttc tgctccgcgc
     7381 tgcccttgtt cgtgggaacc gcggcgctgc ctttgttctc ggcgctgcct ttgttctcga
     7441 ccgtcatcgc gctacctgcc gctgcactgc ccttgttctc gacaggtggc tgcggcggtt
     7501 gcggaggagg ttgcggcggc tgcggaggtg gttgaacttg cgagcctttg tcgacgacct
     7561 gcgagccctc atcgggcttg gcggccgact cggtgccctt cttcttgttg agcagcatga
     7621 acacgccgcc tgctgccagc accacgacga gtgccgcggc gatcacgaac ggcgtcttgc
     7681 tcttctgcgg ctggacctgg cccgcggctg ccgacagcgt cgtcggcgtc gtcaacgtgc
     7741 ccggcaccgg cgagcgcatg ttcacgcccg tcatcggcat gcccgtgggc gtgcccggca
     7801 ccggcgtcat cggcgctggc gtcaaccgca tgggcggcag cggcgcactc gatggcatca
     7861 gctggcgctg catgaacccg gtgaggccgc cgtgcgattc gacgtagccg accgggtcgg
     7921 ccatgccgcg catgaactcg tccatcgtcg ggaagcgcat gtcggggcgc ttctcgagcg
     7981 ccttcagcac gacctgctcg acgtgcggcg acatcatccg gtactgagac ggcggaatcg
     8041 gttgctgcgt gaggtgctgg acgaggatct cgccatagcc ttcgccgatg aacggcacgc
     8101 ggccgacgag catctcgtag agcacgacac ccagcgcgta gatgtcggtg cggtgatcga
     8161 ccgtcgcgcg gccctcgcac tgctccggcg acatataggc cggagtgccg agcacgaggc
     8221 cggtgcgcgt cttgtgcgac gacaggccat cgctgccggt cagcttcgcg atgccgaagt
     8281 cgagcagctt gacgaagtca cgctcgcggc cgcgctggat cagaatgatg ttgtcgggct
     8341 tgaggtcgcg gtgaacgatg ctgttcttgt gcgacgcggc gagcgcatcg gcgacctgca
     8401 tcgcgatcat cagcgcgcgc tcgggcggaa tcggcgcctc agtccggatc gcctgcgaga
     8461 gcgtgtggcc ggcgaggtac tccatgatga agtacacgag ccggtcgcgg ccgcccgggc
     8521 cgctctggat cacgccgtag tcgacgatgt cgacgatgtt cgggtgaccg atcgcgttga
     8581 ccgccttggc ttccgtgaag aagcggtcgg cgacctcggg gttggacgca aactcggcgt
     8641 gaaggatctt gagggcgacc ttcttaccga tggtcgggtg ctcggccaag tagaccgacc
     8701 ccatgccgcc ttcccccaac ttctgggtga ccagatagtt gccgacggtt tgaccgatga
     8761 tgtgatctgg cgaggacgcc ccagcttgtg ggtccatttc cactccgcga gccatcatac
     8821 tagctcacaa gccactgaaa agtcgcggct acgggtccgc cgtgttaact cgatcatgat
     8881 gcgccctttc gtattgtggg tgatcgtggg tgtcctcggc cgtagcgcct tcgcgaacga
     8941 ccttcccgac gtgaaatcgc ggtcggcggt cgtgatcgac tccgaaaccg gcgccgagat
     9001 cttcggcaag gatgccgatg agatccgcgc aatcgcatcg acgaccaaga tctttgtcgc
     9061 gatggctgtg cgcaaaaagg gcatggacct cgatggttgg accgagatca cgcgcgccga
     9121 tgtgcagcat gcacgcggtg gtgcgagaac ccggttggat atccgccgcg tctttcgcaa
     9181 caaagatttg ctgcgcgcca tgctcatggc gtcagacaac cgagcaccga ccgcgcttgg
     9241 gcgcgccgct ggcatggggc ctgacgagct ggtcgccgcg atgaacgaca tcgcgaagga
     9301 cctgcacctc aagcggacca aatttacgga tcccaccgga ttgcgcggca acgtgtcgac
     9361 cgcgcgcgag atggccctcg cgctgcgcgc cgcactggaa gatgacgtgt tgcgcgagat
     9421 catgggcgac ccgttcgagc tcatcgtcgg caaaggcggt cgaccgaaaa tcgcgtacgg
     9481 ctcgacgaat caagcgctcg tcgttcacaa gtacgacgtg atcggcggca agaccggcta
     9541 cacgacggca gccggttatt gcttcgtgac cggggcaagg ttcaacggtc gggaagtggt
     9601 gatgacattc ctcggcgcac cgggcaaatc gagccgattt ggtgatttca atcgcgtggc
     9661 tgcgtggctc gagagcggtg cgccgggcgc gaaagtcaac gtcgcggcga tcgcgaagcg
     9721 accgaagctg aagatcgaag gtgacgtgcg cgggcgcgtc gccaagaagt aggtttaagc
     9781 ttctcgcgtg acttggtcgc ggcgagaggt cctcgcctcc ttaggagttg cttccgcgca
     9841 agccgcgctg tgggcgtgcg gcgcgccggc acagcgcgtt gtcaggcgcg cgaccgaggt
     9901 caaccccgag cttcgcacgt ggctgcacgc tgccgtcgct acgctgaagg gtgccggctt
     9961 cgcgtcggct cacgtgctcg cgacgagccg gcagcgcacc accgccgcga tcgacgtgct
    10021 cggcgccggt gtcgctcgcg cgcgatgtga cgggctcgtg atcacggtgc gcgatcgcga
    10081 cggcatgctg cgcgagcagg tgacgaacga tctatcgcag gccggcatcg cggccgcggt
    10141 caccgtgctc gccggctccg ccaagcccgc gagcgtcgac ttcggcaaga cgcctgcggc
    10201 attttcgcgg ttccgcgccg atcccgatgt cctcgcggac agtgacatcc tcgcgcgcgt
    10261 cgctgcgctc gcgaagcgcg atcgagaatc gctgtcgagc cgcatcgtgt attcggcagc
    10321 ggtgatggat atcgacgacg cgcgcgtgtg gtcggttgca cccggccacg acatgcagca
    10381 gcggctcgtg cacgtgaaga agtcggtgac gcgcgtcgca tggaacggta cgcggccggt
    10441 ggtcagcgaa gtgtcgcgcg cgtgggcggg tggcctcgac gagcaggatc tcgacgacga
    10501 cgagctggtc gccgcacgcg aaggcgcgct cgcgttgatg acgccacgcg cgttcgaaga
    10561 tcacgagtac gcgttcgcgc tcgagcccgc ggtggctgcg gctgtcatcg acgccgccgt
    10621 gcaatcgctg ttcacgccga gcgcggcgcg ccgacccgag gtcgcagcag tgctgccgat
    10681 cggcgcgaag gtgatgtcgc cgatcatcac ggtcgtcgac gatccgggtg ctgcacgtgc
    10741 gtacggtggc ttcgtattcg acgacgcggg cgagcccgca gccgcacgca cgctgatcga
    10801 gcgcggcacg atcgcggcgc ggctggatcg catacggcgt cctggtcacg tcggtcgcag
    10861 cgacccgtac gcctcgcact tgcgagtcac gcccggtgcg atcgagcaga atgacctgct
    10921 cgacgacggc ttcctgctcg aggggccgct cggcgcggtc gtcgatccga cgagtgaccg
    10981 tctcgtgatc tcggtcgcac gcgcgcgcga gcgcgtcgcc ggcaagcgaa ccggccgcat
    11041 gttcgccgag atcgagctcg tcggtgagct gtcgaagatc atgggctccg tcgacgggct
    11101 gtcgaaacag acgaagacga tcggcgtgcg cgatgaggtc gatggccaac cgcgctggcg
    11161 atcgatcgag gcgccgtggg tgcgtggcaa agcgctgctg cgtgcgcgca ggagagcggt
    11221 gtgatcacgc ggcgcgagct tgcgagcacg ctcgcccgac gcggcctcgc cgattgggtc
    11281 gtcgtcgagc ggacacaaga tctcgcgtcg atcgacgagc gcacgaaagt gaagcgcgtc
    11341 gagcgtcgag tcgtgtggtg cgtgaccgtg cacttcgatt cgccgcaggg tcgcggctcc
    11401 gcccacgtga cagtcgacgc ggtggacggt gcagcgagtg cagcggtcga gcaagccgtc
    11461 gcgctcgcgc aatcgtcgat cggtcccgcg tggatctcgc ggcctctcgc cgcacctgcg
    11521 cgcgtcaagc tcgaggacac gtcgctcgcg aagcaagcac cggtcgacat cgcggcggat
    11581 gtgttgaaga agctcgcccg gccaacggcg gtcgccgcac gcgcgacggt gctccgcgag
    11641 cacgtcagtg tcgtcgcgcg gcaaggcttt cacaccgagt ggaccgcgac gctgctctca
    11701 tgcgatgcgc tggtggcggc aaaccagcgc agcttgctga tcgctcgtgg cgcgcgccag
    11761 cgcagcgccc tcgagctcga cactgcagtg acagcggcac agcaggatct cgatcttgtt
    11821 gcgaaggcgg gcgcgccgac ggcaggaccg tgcgcgctcg tgctcgcgac cgacgcgatg
    11881 ctgcacgatg ggctcggcgt atgggcgacg ttcgcgaacc aagccgacgc ggtcgtcgag
    11941 cggcaagggc tcacgcgcta ccgcgagcac acgccgatcg tgccgggtgc cgagcaagtc
    12001 agcgagccgc tcacgatcac gagcgatggc gcgagcgact acggcctgtt gtcggcgccg
    12061 ctcggtgatt acggcgatgc cgtgcgcacg tttccgctcg tcaaccgcgg tgtggccgtc
    12121 ggcctcggac tcacgccgcg tgaaggttcg ctgcgggggc gtgatccgaa cggcggcgtg
    12181 cgtaacctcg tcgtcgagcc cggcggatgg tcgggtgaga tcgacggcag ccagccggtg
    12241 cgcatggtcg aagtccgccg actgcgtagc ctctcgatcg atcc
//
LOCUS       DASUFX010000216         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_588161,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000216 DASUFX010000000
VERSION     DASUFX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588161"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1500
                     /gene="purF"
                     /locus_tag="VFV99_08490"
     CDS             100..1500
                     /gene="purF"
                     /locus_tag="VFV99_08490"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5059383.1"
                     /translation="MCGVFGIHGHDEAANIAYLGMHALQHRGQESAGLVAADDGKLRR
                     HVAMGLVSDAFNRDVLAKLPGSTAIGHVRYSTAGSSELRNAQPFLFEYAGGSVAIAHN
                     GNLVNASELRAELEASGSLFQTSSDTEVIVHLMAKAREPDVLGKLQSALKRLRGAYSL
                     IILTTEGTSSKMIGVRDPNGFRPLVLGRLKDAYVLSSETCSFDLIEADLLREIEPGEI
                     VVIEKGGMTTHRMPESTVVDRTFCVFEHVYFARPDSLVNGKSVYRAREKMGMKLALEA
                     PVEADVVIPVPDSGVPAAIGYSKQSGIPFEMGLIRSHYVGRTFIEPQDSIRHFGVRLK
                     LSPVRSIVDGKRVVVVDDSLVRGTTSRKIVKMLRAAGAKEVHLRISAPPTTHPCYYGI
                     DTPTRSELVAASHSVDEINRYVTSDSLSYLSHEGMMQAVGSETGKGYCSACFTGKYPV
                     ALGQGADLVTLRRAKV"
     gene            1601..2638
                     /gene="prfB"
                     /locus_tag="VFV99_08495"
                     /pseudo
     CDS             1601..2638
                     /gene="prfB"
                     /locus_tag="VFV99_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(2647..2961)
                     /locus_tag="VFV99_08500"
     CDS             complement(2647..2961)
                     /locus_tag="VFV99_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059384.1"
                     /translation="MRAMPNLDTFVTLDDDVLVTATGGQWQMRLPQPGSPGSGPMTLM
                     SLLDKPGKTLDLVRQGLGMPESGPPGSTYIPATANPDGSVNRARLETPRDPNLPQVPL
                     SQ"
     gene            complement(3056..4045)
                     /locus_tag="VFV99_08505"
     CDS             complement(3056..4045)
                     /locus_tag="VFV99_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5059385.1"
                     /translation="MRSRALAFFALAFALIGLGASAASLVDYLNPAPTFCAEAGCATV
                     RQSGWAHPLGVPMPVLGIVYFVAMSVLAFWEKPKLRVALAAVGCAVGIGLIALQAFSI
                     GAWCKLCLIADPAAIASAILIVAGAGTVRATLSNIAATLPAVGLVVLGLGLYAQREDA
                     PNAPVPEFVAKEQKPGQVTIVEFVDFQCPYCRALDQKLTEALGKTQKTVRIVRKMVPL
                     PGHTYAVPAAMAWCSADAQGKGDAMAHELFTANVEDLTPAGCEAIAVRLGCDVTKYRA
                     TFASTELQERIKRDMADGKTLNGLPTIYIGHKKFEGSDHTPDALLAAIERAGS"
     gene            complement(4115..>4852)
                     /locus_tag="VFV99_08510"
     CDS             complement(4115..>4852)
                     /locus_tag="VFV99_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5059386.1"
                     /translation="IALHGNGSTGRQMERLTSFDAVAAREQFVVVYPDAIDHHWNDGR
                     PEFDTAIDDISFVASLIDETAREHAIDRSRVYVTGASNGGMMTYRVGCELADRLAGIA
                     PVIGNLPATIRCEPRVPVSVLAINGTDDPLVPYEGGSIAHGHGRGSVLSATLSTATFA
                     HADGCTGAEATVDEPDIDTSDGSRTRRTQFACPAPLAVELLTLDGAGHTWPGGPQYLP
                     RALIGGTSRDFDGAERIWEFFSAHSRS"
BASE COUNT          869 a         1551 c         1650 g          782 t
ORIGIN      
        1 cgccaaagaa gccgtggcaa gccccgaaaa ggtacgtgaa cacgcgcggt cccacaacat
       61 ttgctacttg ggatcagccc cgcatggtaa gtgagcgcca tgtgcggggt gttcggcatc
      121 catggtcatg acgaagcggc caacatcgcg tacctgggca tgcatgcgct ccagcatcgc
      181 ggccaggaat cggcagggct cgtagccgcc gatgacggca agctgcggcg gcacgtcgcg
      241 atggggctcg tcagcgacgc gttcaaccgg gacgtgctcg cgaaactccc cggttcgaca
      301 gcgatcggcc atgtccggta ctcgaccgcg ggaagctcgg agctgcgtaa cgcgcagccg
      361 ttcctgttcg aatacgccgg tggctcggtc gcgatcgcgc acaacggaaa cctcgtgaat
      421 gcgtccgagc tccgcgcgga gctcgaagcg tccgggtcgc tgtttcagac ctcgagcgac
      481 accgaggtca tcgttcatct gatggcgaag gcgcgcgagc ctgatgtgct cggcaagctg
      541 cagtccgcgc tcaaacgcct gcgcggcgcc tactcgctga tcatcctgac gaccgaaggt
      601 acgagctcca agatgatcgg cgttcgcgat ccgaacggct tccggccgct cgtgctcggc
      661 cggctcaagg atgcgtacgt gctatcgagc gagacctgct cgtttgatct gatcgaagcg
      721 gatctgctcc gcgagatcga gcccggcgag atcgtcgtga tcgagaaggg cgggatgacc
      781 acgcaccgga tgccggagtc gacggtcgtc gaccgcacgt tctgcgtctt cgagcacgtc
      841 tacttcgcgc ggccggactc gctcgtcaac ggcaagagcg tgtaccgcgc gcgagagaag
      901 atgggcatga agctcgcgct cgaggcgccc gtcgaggccg atgtcgtcat tccggtcccc
      961 gactcgggtg tgccggctgc gatcgggtac tcgaaacaga gcggcattcc gttcgagatg
     1021 ggcctcatcc gcagccacta cgtcggccgc acgttcatcg agccgcaaga ttcgatccgt
     1081 cacttcggtg tgcgcctcaa gctgtcgccg gtgcgctcga tcgtcgacgg caagcgcgtg
     1141 gtcgtcgtcg atgactcgct cgtgcgtggc acgacgagcc gcaagatcgt caagatgctg
     1201 cgcgcggcgg gtgcgaaaga agtgcacctg cgtatcagcg cgccgccgac gacgcaccct
     1261 tgctactacg gcatcgacac gccgacccgc agcgagctgg tcgccgcctc gcactcggtc
     1321 gacgagatca atcgctacgt cacgagcgac tcgctctcgt atctttcgca cgaaggcatg
     1381 atgcaggcgg tcggctcgga aaccggcaag ggctattgct cggcgtgctt caccggcaag
     1441 tacccggtgg cgctcggcca gggcgccgac cttgtcactc tccgtcgggc caaggtatag
     1501 acgtacgcca tggcgatcga aggtaccgat gtgcgcgcgg tgcgcgcgca actgaaggag
     1561 ctctccggaa aggtgacaga gctccggagg ttgctttgac gtcgaccaca agcgcctgaa
     1621 gatcgaggag ctcgactccg tcgcggcacg gccgaacttc tgggacgacc agaagaaggc
     1681 gcagacagtc ttgcgcgaca agaaacacct ggagcaggtc gttggcgcgt gggacgcgca
     1741 ggtgcgcgcg ctatcggatg ccgaggtgct gctcgagctc gctgaagaag cgagtgacga
     1801 ggcgagcgcg aaggaagccg cgggcagcgt cgtgacgatc cagaagcagc tcgacgagct
     1861 cgaattcaag cgcatgctgt ccggcgagct cgatggcggc ggtgcgatcg tgcagatcac
     1921 gtcgggcgcc ggcggcgtcg acgcgagtga ctgggcacag atgctcatgc gcatgctgat
     1981 ccgctactgc gagcgcaaag gctggaaggt cgacgtgctc gaagagacgc cggccgaaga
     2041 agccggcatc aagcaggcga cgttcactgt cgccggcgac tacgcgtacg gcttgctcaa
     2101 ggcggagaac ggcgtgcatc gcctcgtgcg cgtgtcgccg ttcgatgcga acgcgcgccg
     2161 gcagacgtcg tttgccgcag tgacggtgac tgccgacatc gacgacgaca tcgaggtgca
     2221 gatcaacgac gtcgatctgc gcatcgacac ctatcgcgca ggcggcgccg gtggccagca
     2281 cgtcaacaag accgactcgg cggtgcgcat cacgcacatg ccgaccggca ttgtcgtgca
     2341 gtgccagaac gagcgcagcc agcacgcgaa caaggacaag gcgttcaagc tgctgcgcgc
     2401 caagctgtac gactacgaga tcgcgaagcg tgaggcgaag gcggccgagg atgcgaagaa
     2461 ccggctcaag atcgagtggg gctcgcagat ccgcagctac gtgttgttcc cgtaccagca
     2521 ggtcaacgat caccgcaccg aggtgaagac gtcggatgtc accggcgtgc tcgacggcga
     2581 tctcgacgag ctcattcgca cgttcttgct gcaggccgcg aacgcgaaga cgaactagcg
     2641 cgagcgctac tggctcagcg gcacttgcgg caggttcgga tcgcgcggcg tctccaagcg
     2701 ggcgcggttg accgagccat cgggattcgc ggtcgcagga atgtacgtgc tgccgggcgg
     2761 accgctttcg ggcatgccga gcccctgccg gaccaagtcg agcgtcttgc cgggtttgtc
     2821 gagcagagac atgagggtca tcgggccgct gccaggcgag cccggctgag gcaggcgcat
     2881 ctgccactga ccgccggtgg cggtgacgag aacgtcgtcg tcgagcgtaa cgaacgtgtc
     2941 gaggttgggc atggcccgca tgccgttcac ggcgtatgcc acgcgcaagt cggttggtcg
     3001 ctgcgctgcc gccgccgacc tgattcctcg agtagacggt actgtgccgt cggggttacg
     3061 aacccgcgcg ttcgatcgcg gcgagtagcg cgtcgggcgt gtggtccgag ccctcgaact
     3121 tcttgtgacc gatgtagatc gtcggcaagc cgttgagcgt cttgccgtcg gccatgtcgc
     3181 gcttgatgcg ctcttggagc tcggtgctcg cgaacgtcgc gcgatacttc gtgacgtcgc
     3241 agccgagccg cacggcgatc gcttcgcaac cggcgggcgt caggtcctcg acgttcgcgg
     3301 tgaacagctc gtgcgccatc gcatcacctt tgccctgcgc gtcggcgctg caccacgcca
     3361 tcgcggcggg aaccgcatag gtatggccgg gcagcggcac catcttgcgg acgatgcgca
     3421 cggtcttctg cgtcttgccg agcgcctcgg tcagcttctg gtcgagcgcg cggcaatacg
     3481 ggcactgaaa gtcgacgaac tcgacgatcg tgacctggcc gggcttctgc tccttcgcga
     3541 cgaattcggg aaccggcgca ttcggcgcgt cctcacgctg cgcgtacagc ccgagcccga
     3601 gcacgacgag gccgacagcg ggcagggtag cggcgatgtt ggacagcgtc gcgcgcaccg
     3661 tgccggcgcc tgcgacgatg aggatcgccg aggcgatcgc agctggatcg gcgatcaggc
     3721 acagcttgca ccacgcgccg atcgagaatg cctgcagcgc gatcagcccg atgccgacgg
     3781 cacagcccac tgcagcgagc gcgacgcgca gcttcggctt ctcccagaat gcgagcacgc
     3841 tcatcgcgac gaagtacacg atgccgagta ctggcatcgg cacaccgagc ggatgcgccc
     3901 agccggattg ccgcaccgtc gcgcaccccg cttcggcgca aaacgttggc gccggattca
     3961 ggtagtcgac gaggctcgcc gcactggcgc cgagcccgat cagcgcaaat gcgagcgcga
     4021 agaatgcgag cgcgcgggag cgcataccgc aagaacatag cgcgaccagg ggcgaattcc
     4081 tcgaccgtct gcaccggcgc gaaattcccg ggagctagct tcgcgaatgc gcgctgaaga
     4141 actcccagat tctctcggcg ccgtcgaagt ctcggctcgt tccgccgatc agcgcgcggg
     4201 gcaagtactg aggcccgccc ggccacgtgt gacctgcgcc gtcgagcgtg agcagttcga
     4261 ccgcgagagg cgccggacac gcgaactgcg tgcgtcgcgt gcgcgacccg tcgctggtgt
     4321 cgatgtcggg ctcgtcgacg gtcgcctcgg ctcccgtgca gccgtcagcg tgcgcaaacg
     4381 tcgcggtcga gagcgtcgct gacagcacgc tgccgcggcc atgaccgtgc gcgatactgc
     4441 cgccctcgta cggcacgagt ggatcgtcgg tcccgttgat cgcgagcacc gagacgggca
     4501 cgcgcggttc gcagcgaatc gtcgccggta gattgccgat caccggtgcg atgccggcga
     4561 gccgatcggc gagctcgcag ccgacgcggt aggtcatcat gccgccgttc gaggcgccgg
     4621 tgacgtagac gcggctgcga tcgatcgcgt gctcgcgcgc ggtctcgtcg atcagcgacg
     4681 cgacgaagct gatgtcgtcg atggcggtgt cgaattcggg ccggccatca ttccagtgat
     4741 gatcgatcgc gtcgggatac acgacgacga actgctcgcg cgcagcaacg gcatcgaagc
     4801 tcgtgagccg ctccatctgc cggccggtgc tgccattgcc atgcagcgcg at
//
LOCUS       DASUFX010000217        12291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_32822,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000217 DASUFX010000000
VERSION     DASUFX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32822"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFV99_08515"
     CDS             <1..738
                     /locus_tag="VFV99_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5059387.1"
                     /translation="PIISIRNLNKVYASGFQALKNVNLEIRRGEIFALLGPNGAGKTT
                     LIGIVCGIVNASGGRVTVDGHDIVSDYRAARALIGLVPQELTTDAFETVWDTIRLSRG
                     LWGLPPDASHLEKVLKSLSLWDRKDAKIMTLSGGMKRRVMIAKALAHEPQILFLDEPT
                     AGVDARGRAELLELMTGLSRSDELAAILVTHNLAAVAQTAERIVYIEAGRVLCWGLPQ
                     ELLGQESLTALAAFQAADHHAHARDEE"
     gene            735..1565
                     /locus_tag="VFV99_08520"
     CDS             735..1565
                     /locus_tag="VFV99_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5059388.1"
                     /translation="MMGFLTEPLAYEFMQRALLAGIAVAAVCAVIGVFIVQRGLAFLG
                     DGLAHAAFGGIAIGLYLGASIEHAVWIAIPFTALIALGIGYVLRRSALRGDVATGVFF
                     AFAFALGVLFLGLRGASDRPVQVEHLLFGNMLSVTPTVLHVVVAVAVLACVITFVLWS
                     RFAYAIFDPELAAISGVPVAALEYLLLAQTAVVVVVAVKTVGVVLVSSFVVIPAATAR
                     LVGTSLATATILALVIGTVGAAVGLIASYHLNTPAAATIILIHSAGFAGAFAWSHIRR
                     "
     gene            1562..2782
                     /locus_tag="VFV99_08525"
     CDS             1562..2782
                     /locus_tag="VFV99_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5059389.1"
                     /translation="MKNARIGVVGCGTAGAAAAILFARAGNHVTVLERVAAPGPVGAG
                     IMIQPTGQAVLGRLGLLDAIAAKGARIDRLWFRTHTGRTLVDLHYAKVDQAWFGIGLH
                     RGVLFEALYNAARHEPRVTVHTGCAVTGLRRDGDQTWLVAPDTEHGPFDLVIVADGSV
                     SELRNHAGETKRDAAYPWGALWFVADDREGVFQRELYQVGVRARRLYGVLPTGHAPRG
                     QGPVVSLFWSLAASELDTWRAGGLAAWKAEVAAIDPRVQFVLDQIDSVEQITFARYRD
                     VQMTRWHDRRVVFIGDAAHATSPQLGQGANLALVDALVLADALAAAPSLDAGLDQYVQ
                     NRRGHLRYYQRMTRWLTPLFQSSSQLLGWFRDWTFPIANRVPPLRNLMIKTMAGVSMG
                     FVARPLKLPLSLKP"
     gene            2914..4527
                     /locus_tag="VFV99_08530"
     CDS             2914..4527
                     /locus_tag="VFV99_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059390.1"
                     /translation="MDGPVDPLPPPGKEDSQYRKGLLVATNTSRTQVWTARNKWEDTD
                     TPAARAAGLAWTANSGLTWDQKYAAWLDSLEWVPSLDNYSTTVRITTPWGKSLASPYL
                     ECAEMGLFLRITFAAWYELPLQLEAISNGQRVYFGHYGVRTAAGRFSGTPEFAIQYKD
                     YTSSTTWKTNWPKDNTLRSRKLWGGTDEQPEVGANQVFGAYVDEIHLNKRAAYFTVLA
                     LNYLSSANLADTANTYNIVPEAVRPGDILLERWQRSGIGHTLVVKEVVPISETSLDVT
                     TISGSMPRRQGVKQSGQSSKSYFTSQYTGGDELSDDGTPYSKLGGGLKRFRVTKNVGG
                     YWTNTWMQADEAHWINSLDYARIGARPARFEALLGQVSPQQMKTELLAQIADARHHLE
                     QYPASCSARERRETAFNALYSIAYQLNTTRAALDKQYRTDADYVLGELEYTKSKTCCW
                     NSSTAGMYDLIMKKAAADKASADARHVCEVPTVFAARSDGYKLWSDYAASVGRASEWK
                     AWTEDESCPQKNVAMDTQAELSATPYCSL"
     gene            complement(4528..5364)
                     /locus_tag="VFV99_08535"
     CDS             complement(4528..5364)
                     /locus_tag="VFV99_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5059391.1"
                     /translation="MNEQRMEFYSREREVSGWETWHDWHEMVMRPVADWLVRASGAAA
                     GQKILDVACGTGIPGLLLAEAVGATGKVVGIDISPKMLTGMRNKAAARGLRNIETREV
                     DALTFGGPDAVFDAVTCKDGFMFFPDIVAGVRELRRVVKPGGRYAFSAWAEPQANPAF
                     ATLFGPLAEALKLPPPAPDTPGPFRLAAPGALERVVRDAGFTDVTVERVAITWTFASV
                     AQHWEIFSVMAGPLERAIATLPESELAQLKQKLAESIAPFATADGGLRFPGVEWCVSG
                     TR"
     gene            5460..6371
                     /locus_tag="VFV99_08540"
     CDS             5460..6371
                     /locus_tag="VFV99_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5059392.1"
                     /translation="MDTVDGMRVFVRVAQRASFAAAARELRLSPAAVTKQVAALEARV
                     GARLLDRTTRRVAMTEAGRVYLERCLECLQTFDDADASVSELSVAPRGQLRISAPVDM
                     HSQLPPVLAEFARTYPDVRIDLQLSNRAIDLVDEGFDLAIRAAPMLDGRHVARPLADL
                     RLGVYASPQYLRAHGRPRKPADLARHRALVFVEPRLRNTWMFERDGKQTSVEVNATIT
                     GNSGDVLREMTTAGLGVFMAPSMVVDRAVAARQLERVLREWTVLPKPKLWAVYPHRRF
                     LPAKVRLFVEALRAAFGGDPDRDPWWT"
     gene            6413..8008
                     /locus_tag="VFV99_08545"
     CDS             6413..8008
                     /locus_tag="VFV99_08545"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5059393.1"
                     /translation="MSSTMMDVPLTLDWIADRAERYMGSVEVVSRRPDKSISRSNYAQ
                     VIASSRRLARALVAAGIKKGDRVATLMWNHAEHLEVYFGIPLSGAAIHTLNLRLHPDE
                     IAFIANDAKDRFVIVDDVLLPLLDKVIAAGGTFERVIVVGNAGDRESYTSFLDKAPAQ
                     TALPVLAETDQLGVCYTSGTTGRPKGVVYSHRSTILHAIVGGLPDCLDLSRADTLMPV
                     VPMFHVNAWGLPYGAAMVGAKLVCPGPHLDPPSLLDLMERERVTIAAGVPTIWIGIRD
                     VLDANPGKYKLQPNVRMVVGGSAAPEQLIRDFDRHGMTLIHAWGMTETSPIGTVSRVP
                     PELEHADEATRIALRARQGRPVPLVDIRVRNENGDVPHDDTTSGEVLIRGPWIAAAYA
                     GGHTPERWTSDGYFRTGDVARVDQHGFLKLTDRIADLIKSGGEWIASQEIENALMGHP
                     AVREAAVIGVPHPKWSERPLAAVVLKDGQMVTADELRDFLAPRFAKFWLPDAFVFVDA
                     IPRTSAGKFKKTELREQYREWKW"
     gene            complement(7907..9100)
                     /locus_tag="VFV99_08550"
     CDS             complement(7907..9100)
                     /locus_tag="VFV99_08550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5059394.1"
                     /translation="MATRVVAGAWLVGLAAAGCTDARTDTSEVQQRIVGGTPTTGDPA
                     VVAVARRRIGCGDMSVVTCSGVLVAPRVVLTAAHCVTGSTRGALEVIFGNDAAAPSTM
                     IVVASAQAYAGYDPSTGDGDIAALLLADDAPVAAIARPTGTIMDVTAGATLRAVGFGV
                     TAWTATDPGVKREGSLALGEVRAGSFDATPSPAMTCTADSGGPVFATINANEELVGLT
                     SRGDAACATTAVNARVDVAQSPFVDPFVTASASAPPGWPAGLPPIDALATTSCTTDAE
                     CPALMTCSVFDHHCGFAWLGAGSFGAACANDADCGAAPARCARVWPDGADACRCFTSS
                     MQPPGPDAGTGSGELPPNGCDGCAGSGGDALPLPFAILLAQLGLLELSRRRARNRVDK
                     HERVR"
     gene            complement(9082..9453)
                     /locus_tag="VFV99_08555"
     CDS             complement(9082..9453)
                     /locus_tag="VFV99_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059395.1"
                     /translation="MRFAALARLAAVVVVLAGCSSSHDTELDGARDQVRRFFDAIGTS
                     DCPTLELLVPAAREPGACEHLVHEWRDELHIKLLEVPDVRRDGRDRKAIIVRATVLRR
                     DQQQTMLVRVTHEQGAWQLVL"
     gene            complement(9450..9947)
                     /locus_tag="VFV99_08560"
     CDS             complement(9450..9947)
                     /locus_tag="VFV99_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059396.1"
                     /translation="MRRLAGIVAAALLIVVGVLWWTRGGDRSTATRAATSTAVSVSKA
                     PAPARPPTLASANGTDPAEEPRPQPVPVDPATAPSLDAPVSKPAPPQKPFDRAETIAK
                     READLKLLDDTRTRLEKELASAQAAGDATAVHDLQIRITRTSELRKQRSGELDKLRAG
                     GELPK"
     gene            complement(9935..10870)
                     /locus_tag="VFV99_08565"
     CDS             complement(9935..10870)
                     /locus_tag="VFV99_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059397.1"
                     /translation="MLKLSTYRIGIGLLAAFAGCTSDEPTSNEPTTSASTELEIHDTS
                     SGVAGSLGETTFTSSMVNGDVLEITVKVNCMMVTALVDYKTGVMEFDGFALDTGENTQ
                     MTDEDRAALTELEQALAPLGEDLPFTLEKLRGFVATYAEHPSTVDLQTIRITEENRSW
                     TSICYAKNSYYPASHDCDVDGWWVDRTTLDNVYLSSTGNLGGPDGVAWYFPWGGGFKC
                     CSGSLWCSGYTCNIPYAGWWSGVEVNHDVNVEHAYGNCFGVCGAGCPGSYQYTVDCLN
                     HDSCVRNGHVLASGYCDDQYTSSLDDWASAPNCGA"
     gene            11078..11881
                     /locus_tag="VFV99_08570"
     CDS             11078..11881
                     /locus_tag="VFV99_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059398.1"
                     /translation="MSEPWDTAAAIAAWAPDATRELLANAVDLEDEFALPRASELMAK
                     AFAVTGVWDLARDTYALAADTWEALDAARARALRELVQLLPLVGFANEHEAIEAERML
                     VRSLDARGVPLLVLRALTEIRRAILPRQVTGLIEATFELAPMQHAFVMALALDSAARL
                     TMHEWQMRLRPSVWFEHSLERTIELGLVHTTPVVTAHPRLVSRLHGRTVIEMPRGVRY
                     EELEPTQRGLRRFRLHAIGNPLPEALLEAAIEIGVHGGVVADDGVSSPP"
     gene            11878..>12291
                     /locus_tag="VFV99_08575"
     CDS             11878..>12291
                     /locus_tag="VFV99_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059399.1"
                     /translation="MTAAETLLAEAVAAVNAGDYKTAANRFERAGELLSAKSGKKLGK
                     GRARDAAAAYESAARCYLMLNDHNHAIAAVELARRLQPASPRVARVHAEVADRVGYAP
                     TRRQAWQDVLTAGRRSDRMFAQLQLASIARELGEHA"
BASE COUNT         2009 a         4239 c         4002 g         2041 t
ORIGIN      
        1 ccgatcatat cgatccgcaa tctcaacaaa gtctacgcct ccggctttca ggcgctgaag
       61 aacgtcaacc tggaaattcg ccgcggcgag atcttcgccc tgctcggccc gaacggcgcg
      121 ggcaagacca cgctgatcgg catcgtttgc ggcatcgtca acgccagcgg ggggcgggtg
      181 acggtggacg gtcacgatat cgtgtccgac taccgggccg cccgcgcgct gatcgggctg
      241 gtgccgcagg aactcaccac cgacgccttc gaaacggtgt gggacacgat ccgcctgagc
      301 cgcggcctgt ggggtctgcc acccgacgcc tcccacctcg agaaggtgct gaaatcgctg
      361 tcgctgtggg acaggaagga cgccaagatc atgacgctgt ccggcggcat gaagcgccgc
      421 gttatgatcg ccaaggcgct ggcgcacgag ccgcagatcc tgttcctcga cgagccaacc
      481 gcaggcgtcg atgcgcgcgg ccgtgccgag ctgctcgagc tgatgaccgg cttgtcgcgg
      541 tccgacgagc tcgccgcgat cttggtcacc cacaacctcg ccgccgtcgc ccagacggcc
      601 gagcgcatcg tctacatcga ggccggtcgc gtgctgtgct ggggcctgcc tcaagagcta
      661 ctcggccagg aatcgctgac ggcgctcgcc gcgttccagg cggctgacca ccacgcgcac
      721 gcccgcgacg aggaatgatg ggcttcctca ccgagccact cgcgtacgag ttcatgcagc
      781 gcgcgctgct cgcaggcatt gcagtcgccg cggtgtgcgc ggtgatcggc gtgttcatcg
      841 tccagcgcgg gctcgcgttc ctcggcgatg gcctggcaca cgctgcgttc ggcggcatcg
      901 cgatcgggct ctatctcggc gcttcgatcg aacatgcggt atggatcgcg attccgttca
      961 ccgccttgat cgcgctcggc atcggctacg tgctgcgccg ctccgcgttg cgcggcgatg
     1021 tcgcgaccgg cgtgttcttc gcgttcgcgt tcgcgctcgg cgtgctgttt ctcgggctgc
     1081 ggggggcgtc cgatcgccca gtgcaggtcg agcacttgct gttcggcaac atgctctcgg
     1141 tgacgccgac cgtgctgcat gtcgtcgtcg cggtcgccgt gctcgcatgc gtgatcacgt
     1201 tcgtgctgtg gtcgcgcttc gcctatgcga tctttgatcc cgagctcgcg gcgatctccg
     1261 gcgtgccggt cgctgcgctc gagtatctgt tgctcgccca gactgccgtc gtcgtggtcg
     1321 tcgcggtcaa gacggtcggt gtcgtactcg tgtcgagctt cgtcgtgatt cccgcggcga
     1381 ccgcgcggct cgtcggcacc tcgctcgcca cggcgacgat cctcgcgctc gtcatcggta
     1441 ccgtcggtgc ggccgtcggc ctcatcgcga gctatcacct caacacgccc gcagcggcga
     1501 ccatcatctt gatccatagc gcaggcttcg cgggcgcctt cgcgtggtct catatccggc
     1561 ggtgaagaac gcccgtattg gtgtggtcgg gtgcggcacc gcgggcgccg ctgctgcgat
     1621 cttgttcgca cgcgctggca accacgtcac cgtgctcgag cgtgtcgccg cgcccggccc
     1681 agtcggcgcc ggcatcatga tccaaccgac gggccaggcg gtgctcggcc gactcggctt
     1741 actcgacgcg atcgcagcga agggcgcgcg catcgatcgc ctctggttcc gcacgcacac
     1801 cgggcgcacg ctcgtcgatc tgcattacgc aaaggtcgat caggcgtggt ttggcatcgg
     1861 cctgcatcgc ggcgtgctgt tcgaagcgct ctataacgcc gctcgtcacg agccgcgcgt
     1921 gaccgtgcat accggctgtg ctgtcaccgg gctccgccgc gacggcgacc agacctggct
     1981 cgtcgcaccg gacaccgagc acggcccgtt cgatctggtg atcgttgccg acggctcggt
     2041 gtccgagctg cgcaaccacg ccggcgagac gaaacgcgat gccgcgtatc cgtggggcgc
     2101 gctgtggttc gtggccgatg atcgcgaggg cgtgttccag cgcgagctgt atcaagtcgg
     2161 cgtacgcgcg cgccggctct acggcgtgct gccgaccggt cacgcaccgc gcggtcaggg
     2221 accggtcgtg agtttgttct ggagcctcgc agcgagcgag ctcgacacgt ggcgcgcagg
     2281 cggactcgca gcgtggaaag ccgaagtcgc cgcgatcgat ccacgcgtcc agttcgtgct
     2341 cgatcagatc gactcggtcg agcagatcac gttcgcgcga tatcgcgacg tccagatgac
     2401 gcgctggcac gatcgccgcg tcgtgttcat cggcgatgcc gcacacgcga cgagcccgca
     2461 actcggccaa ggcgccaacc tcgcgctcgt cgatgcactc gtgctcgccg acgcacttgc
     2521 cgccgcaccg tcgctggacg ctggcctcga ccagtacgtg cagaatcggc gcggccacct
     2581 tcgctactac cagcggatga cgcgctggtt gacgccgctg ttccagtcga gctcgcagct
     2641 gctcggctgg tttcgcgact ggacgtttcc gatcgcgaac cgcgtcccgc cgctgcgtaa
     2701 cctgatgatc aagacgatgg ctggagtctc catgggcttc gtggcccgac cactgaagtt
     2761 gccactttcg ctcaagccct gaagatcacg aggcctgacc acggcacacc gcctgctgag
     2821 gggaacggcg ccatcatgcg catccttgct tcccttctag tttgcttaat cgcctgtacc
     2881 gatacgtccg gcggccccga cgaggaatgg ggcatggacg gtcccgtcga cccgttgcca
     2941 ccccctggca aagaagactc gcagtaccgc aagggactgc tcgtcgcgac caacacctcg
     3001 cgcacgcagg tgtggaccgc gaggaacaaa tgggaagaca ccgacacgcc ggccgcgcgc
     3061 gccgccggtc tcgcgtggac cgcgaacagc ggcctcacct gggatcagaa gtacgccgcg
     3121 tggctcgact cgctggagtg ggtcccgtcg ctcgacaact attcgacgac cgttcgcatc
     3181 acgacgccgt ggggcaagtc gctcgcgtcg ccgtacctcg agtgcgccga gatgggcttg
     3241 ttcctgcgca tcacgtttgc tgcctggtac gagctgccac tgcagctcga agcgatctcg
     3301 aatggccagc gcgtctactt cggtcactac ggcgtgcgca cggccgcggg tcgcttctcc
     3361 ggcacgcccg agttcgcgat ccagtacaag gactacacgt cgagcacgac gtggaagacg
     3421 aactggccga aggacaacac gctgcgctcg cgcaagttgt ggggcggcac cgacgagcag
     3481 cccgaggtcg gcgcgaacca ggtgttcggc gcgtacgtcg acgagattca cctcaacaag
     3541 cgcgcggcgt acttcaccgt gctcgcgctg aactacctgt cgtcggcaaa cctcgccgac
     3601 accgcgaaca cgtacaacat cgttcccgaa gcggtgcgcc ccggcgacat cctgctcgaa
     3661 cgctggcagc gctccggtat cggtcacacg ctcgtcgtca aagaagtggt gccgatcagc
     3721 gagaccagcc tcgacgtcac cacgatctcg ggctcgatgc cgcgccgcca gggcgtcaag
     3781 cagtcggggc aatcgtcgaa gagctacttc acgagccagt acaccggcgg cgacgagctg
     3841 tcggatgacg gcacgccgta cagcaagctc ggcggcggtc tcaagcggtt ccgcgtgacg
     3901 aagaacgtcg gcggctactg gacgaacacg tggatgcaag cggacgaagc gcactggatc
     3961 aactcgctcg actacgcacg catcggtgcg cgccctgccc gcttcgaagc gctgctcggc
     4021 caagtctcgc cacagcagat gaagaccgag ctgctcgcgc agatcgccga cgcacgtcac
     4081 cacctcgagc agtacccggc atcgtgctcg gcccgcgagc gccgtgagac cgcattcaac
     4141 gcgctctact cgatcgcgta tcagctcaac accacgcggg ccgccctcga caagcagtac
     4201 cgcaccgatg ccgactacgt gctcggcgag ctcgagtaca cgaagagcaa gacgtgctgc
     4261 tggaacagct cgaccgcggg aatgtacgac ctgatcatga agaaggcggc tgccgacaaa
     4321 gcatcggccg atgctcgcca cgtctgtgaa gtaccgacgg tcttcgccgc gcgcagcgac
     4381 ggctacaagc tgtggtcgga ctacgccgcc tcggtcggcc gcgcctccga gtggaaggcg
     4441 tggacggagg acgaatcgtg tcctcagaag aacgtcgcga tggacaccca agccgagctg
     4501 tccgcgacgc cgtattgctc gctgtgatca gcgcgtaccg gacacgcacc attcgacgcc
     4561 cgggaagcgc aggccgccat ccgccgtcgc gaacggcgcg atcgattcgg cgagcttctg
     4621 cttgagctgc gccagctcgc tctccggcag cgtcgcgatc gcacgctcga gtggccccgc
     4681 catcacgctg aagatctccc agtgctgggc gaccgacgcg aacgtccacg tgatcgcgac
     4741 gcgctcgacg gtgacatcgg tgaagccggc gtcgcgcacg acgcgctcga gcgcacccgg
     4801 cgcggcgagc cggaatggcc ccggcgtatc cggagccggc ggcggcagct tcaatgcttc
     4861 ggcgaggggg ccaaacagcg tcgcgaacgc cgggttcgct tgtggctccg cccacgccga
     4921 gaacgcatag cgcccgcccg gcttgaccac gcgccgtagc tcgcgcacgc ccgcgacgat
     4981 gtcggggaag aacatgaagc cgtccttgca ggtcacggcg tcgaagaccg cgtcgggacc
     5041 gccgaacgtc agtgcgtcga cctcgcgcgt ctcgatgttg cggagcccgc gcgcggcggc
     5101 cttgtttcgc attccggtca acatcttcgg agaaatgtcg atccccacga ccttgccggt
     5161 cgcaccaacc gcctcggcga gcaggaggcc cgggatcccc gtaccgcacg cgacatcgag
     5221 aatcttctgt ccggcggcag cgccggatgc gcgcacgagc cagtccgcaa ccgggcgcat
     5281 gaccatctcg tgccagtcgt gccacgtctc ccaaccactg acctcgcgct ctcgcgagta
     5341 gaactccata cgctgctcgt tcatctcgtg cctccacgaa caaggtagtc gtctgctcgc
     5401 cacgccggaa tacctacgag ggaactggat cgtttccaaa ttggaaataa tatcggccga
     5461 tggacacggt cgatggcatg cgggtcttcg tgcgcgtggc gcaacgcgcg agctttgccg
     5521 ccgcagcgcg tgagctgcgg ctctcacccg ctgcggtaac gaagcaggtc gccgcactcg
     5581 aagcgcgcgt cggcgcacgc ctcctcgacc gcaccacacg cagggttgca atgacagaag
     5641 ccgggcgtgt ctacctcgag cgctgcctcg agtgcctgca gacgttcgat gacgccgacg
     5701 cctcggtgag cgagctctcg gttgcaccgc gcggccagct ccgcatcagc gcgccggtcg
     5761 acatgcattc ccagctgccg cccgtgctcg ccgagttcgc gcgcacatat cccgatgtcc
     5821 gcatcgacct gcagctctcg aatcgcgcga tcgatctcgt cgacgaaggc ttcgacctcg
     5881 cgattcgcgc agcaccgatg ctcgacggcc gccacgttgc ccggccgctc gctgatctac
     5941 gcctcggcgt ctacgcatcg ccgcagtatc tgcgcgcgca cggtcgacca cgcaagcccg
     6001 cagacctcgc acggcaccgc gcgctcgtgt tcgtcgaacc gcgcctacgt aatacgtgga
     6061 tgttcgagcg tgacggcaag caaaccagcg tcgaggtgaa tgccacgatc accggcaact
     6121 cgggagacgt cctacgcgag atgacgactg ccgggctcgg cgtcttcatg gcaccgagca
     6181 tggtcgtcga tcgcgcggtg gccgctcggc aactcgagcg cgtgctccgc gaatggacgg
     6241 tcttgccgaa accgaagctt tgggccgtgt acccacaccg gcggttcttg ccggcgaagg
     6301 tgaggctgtt cgtcgaggca ctgcgagcgg cgttcggtgg tgacccggac cgcgatccat
     6361 ggtggacctg aggtagcctc ccagtgcgga accacttgcg gaggagaatc gcgtgagctc
     6421 gacgatgatg gatgtgccgc tgaccctcga ttggatcgcg gaccgtgcag agcgctacat
     6481 gggcagtgtc gaggtcgtgt cgcgcaggcc cgacaagagc atctcgcgct cgaactacgc
     6541 gcaggtgatc gcgagctctc gccggctcgc ccgcgcgctg gtcgcggccg gcatcaagaa
     6601 gggcgatcgg gtcgcgacgc tgatgtggaa ccacgccgag cacctcgagg tctacttcgg
     6661 cattccgctg tcaggcgccg cgatccacac gctgaacctg cgcctgcatc ccgacgagat
     6721 cgcgttcatc gcgaacgatg cgaaggatcg cttcgtcatc gtcgacgacg tcttgctgcc
     6781 attgctcgat aaggtcatcg cggccggcgg tacgttcgaa cgcgtgatcg tggtcggcaa
     6841 cgctggcgat cgcgagagct acaccagctt cctcgacaag gcgcccgcgc agaccgcatt
     6901 accggtgctc gccgaaaccg accagctcgg cgtctgctac acgagtggca cgaccggtcg
     6961 cccgaagggc gtcgtctact cgcatcgctc gacgatcctg cacgcgatcg tgggcggcct
     7021 gccggattgt ctcgacttgt cgcgcgcgga cacgctgatg cccgtcgtac cgatgttcca
     7081 tgtgaacgcg tggggcctgc cgtacggtgc cgcgatggtc ggcgccaagc tggtgtgccc
     7141 gggtccgcac ctcgacccgc cgagcctgct cgatttgatg gagcgcgagc gggtgacgat
     7201 cgcggccggc gtaccgacga tctggatcgg catccgcgac gtgctcgacg cgaacccggg
     7261 caagtacaag ctccagccga acgtccgcat ggttgtcggc ggctcggccg cacccgagca
     7321 gctgatccga gacttcgatc gtcacggcat gacgctgatc cacgcgtggg gtatgacgga
     7381 gacgtcgccg atcggcacgg tctcgcgcgt gccaccagag ctcgagcacg ccgacgaggc
     7441 gactcgcatt gcgctgcgcg cccgacaggg ccgccccgtt ccgctcgtcg acattcgcgt
     7501 gcgcaacgag aacggcgacg taccgcacga cgacacgacg agcggtgaag tgttgattcg
     7561 cggcccgtgg atcgcggcag cgtacgccgg cgggcacacg ccggagcgct ggaccagcga
     7621 cggctacttc cgcactggcg acgtcgcgcg cgtcgaccag cacggcttcc tcaagctcac
     7681 cgatcgcatc gccgacctca tcaagtcggg cggcgagtgg atcgcatcgc aagagatcga
     7741 gaacgcactg atgggacacc ccgcagtgcg cgaggcggca gtgatcggcg taccgcaccc
     7801 gaagtggagc gagcgcccgc tcgcggcagt cgtgctcaag gacggtcaga tggtgaccgc
     7861 cgacgagctg cgcgacttcc tcgcgccgcg cttcgcgaag ttctggctac cggacgcgtt
     7921 cgtgtttgtc gacgcgattc cgcgcacgtc ggcgggaaag ttcaagaaga ccgagctgcg
     7981 cgagcagtat cgcgaatgga agtggtagcg cgtcgccgcc acttccggcg cagccgtcac
     8041 agccgttcgg cggtagctcg ccgctgccgg tgcccgcgtc cggtcccggc ggctgcatcg
     8101 acgacgtgaa gcagcggcac gcgtctgcgc cgtcgggcca cacacgcgcg cagcgcgccg
     8161 gcgcggctcc gcagtcggcg tcgttcgcgc acgcggcgcc aaagctcccc gcgccgagcc
     8221 acgcaaaccc gcagtggtga tcgaacaccg agcacgtcat cagcgcggga cactcggcgt
     8281 cggtcgtgca cgaggtcgtg gcgagcgcgt cgatcggcgg caatccggcc ggccagcccg
     8341 gcggtgcgct cgcgcttgcg gtcacgaacg gatcgacgaa cggcgactgc gcgacgtcga
     8401 cgcgagcatt gaccgcggtt gtcgcgcacg ccgcatcgcc acgcgacgtc agcccgacga
     8461 gctcctcgtt cgcgttgatc gtcgcaaaca ccggcccgcc gctgtcggcg gtgcaggtca
     8521 tcgccggcga cggcgtcgcg tcgaagctcc ccgcccgcac ctcacccagt gcgagtgagc
     8581 cctcgcgttt cacaccggga tcggtcgcgg tccatgccgt cacgccaaag ccaactgcac
     8641 gcaacgtggc gccggccgtg acgtccatga tggtccccgt gggccgggcg atcgccgcga
     8701 ccggcgcgtc gtcggcaagg agcagcgcgg cgatgtcgcc atcgccggtc gacggatcgt
     8761 agccggcgta ggcctgcgcc gacgcgacga cgatcatcgt gctcggcgct gccgcatcgt
     8821 tgccgaagat cacctcgagt gcgccgcgtg tcgatcccgt cacgcaatgc gccgcggtga
     8881 gcaccacacg tggagcgacg agcacgccgg agcacgtcac gacagacatg tcaccgcaac
     8941 cgatccgccg ccgtgcgaca gcgacgaccg ccggatcgcc tgtcgtcggg gtgccaccga
     9001 cgatgcgctg ctgcacctcg ctcgtgtccg tccgcgcgtc ggtgcagcca gcggcggcaa
     9061 gcccgacgag ccacgcgccc gctacaacac gagttgccac gcgccctgct cgtgagtcac
     9121 ccgcacgagc atcgtctgct gctggtcgcg ccgcaacacg gtcgcgcgga cgatgatcgc
     9181 cttgcgatcg cggccatcgc gccgcacgtc cggcacctcg agcagcttga tgtgcagctc
     9241 gtcacgccac tcgtggacca ggtgttcgca ggcgcccggc tcgcgcgctg cgggtaccaa
     9301 cagctcgagt gtcgggcagt cgctcgtgcc gatcgcgtcg aagaagcggc gcacctgatc
     9361 gcgcgcgcca tcgagctcgg tgtcgtgcga gctgctgcag cctgcaagca ccacgacgac
     9421 cgcagcgagc cgtgcgagcg cagcgaacct cactttggca gctccccgcc cgcgcggagc
     9481 ttgtcgagct cgccgctacg ctgcttgcgc agctctgacg tccgcgtgat ccggatctgc
     9541 agatcgtgga cggcggtcgc atcaccggcg gcttgcgcgg acgccagctc cttctcgagc
     9601 cgcgtgcgcg tgtcgtcgag cagcttcaga tcggcctcgc gcttcgcgat cgtctcggcg
     9661 cgatcgaagg gcttctgtgg cggcgccggt ttgctgaccg gtgcgtcgag cgacggcgca
     9721 gttgccggat cgaccggcac cggctgcggc ctcggctcct ccgcagggtc ggtgccgttc
     9781 gcgctggcga gcgttggcgg gcgcgcaggt gccggcgcct tcgataccga taccgccgtc
     9841 gacgtcgctg cacgcgtagc agtgctccga tcgccgccgc gggtccacca caagacgccg
     9901 acgacgatca gcagtgctgc cgcgacgatg cccgctaggc gccgcaattg ggagccgatg
     9961 cccagtcgtc caaggacgag gtgtattggt cgtcgcagta gccactcgcg agcacgtggc
    10021 cgttacgtac gcacgaatcg tggttcagac agtcgaccgt gtactggtac gagcccggac
    10081 acccggcgcc gcacacgccg aagcagttac cgtatgcgtg ctcgacgttg acgtcgtgat
    10141 tgacttcgac accgctccac cagcccgcgt acgggatgtt gcacgtgtag ccgctgcacc
    10201 agagcgagcc cgagcagcac ttgaagccgc cgccccacgg gaagtaccac gcgacaccat
    10261 ccgggccgcc gaggtttccg gtcgagctca ggtagacgtt gtcgagcgtc gtccgatcga
    10321 cccaccagcc gtcgacgtcg cagtcgtggc tcgccgggta gtagctattc ttcgcgtagc
    10381 agatgctcgt ccagctgcga ttctcctcgg tgatccggat cgtctgcaga tccaccgtcg
    10441 acgggtgctc cgcatacgtg gcgacgaagc cgcgcagctt ctcgagcgtg aacggaagat
    10501 cctcgccgag cggtgcgagc gcttgctcga gctcggtgag cgcggcgcga tcctcgtccg
    10561 tcatctgtgt gttctcgccg gtgtcgagcg cgaaaccgtc gaactccatc acaccggtct
    10621 tgtagtcgac gagcgcggtg accatcatgc agttgacctt gaccgtgatc tcgagtacgt
    10681 ccccgttcac catcgagctg gtgaacgttg tctcgccgag actcccggcc acgccacttg
    10741 aggtgtcgtg gatctcgagc tctgtggatg ccgacgtcgt cggctcgttg ctggtgggct
    10801 cgtcggatgt acagccggcg aacgctgcga gtagcccgat cccgattcga tatgtgctga
    10861 gcttgagcat tggcccctcc gttcggatga agacccgccc ctcccgatgg cttccccaaa
    10921 gtccatctag ggtgaaacac tcccgtaagc gcaatggcga attttctagg caattagccc
    10981 aagaatgcgg agttacggag cgctggcgcg taatctctgt gaagtcactc acttcaaaca
    11041 cactcgcacc gactcttcct caccttcccg tttgaccatg tccgaacctt gggacacagc
    11101 tgctgcgatc gccgcgtggg cgcccgacgc tacccgtgag ctccttgcga acgctgtcga
    11161 tctcgaggac gaattcgcgc tgccgcgcgc gagcgagctg atggcaaaag cattcgcggt
    11221 caccggcgtg tgggatctcg cgcgcgacac gtacgcgctc gccgccgata cctgggaagc
    11281 actcgatgcg gcacgcgcgc gcgcactgcg cgagctcgtg cagctgttgc cgctcgtcgg
    11341 atttgcgaac gagcacgagg cgatcgaagc cgagcgcatg ctcgtgcgtt cgctcgacgc
    11401 acgtggcgtg ccgctgctcg tgctgcgcgc gctcaccgag atccgacgcg caatattgcc
    11461 gcggcaggtc accggcctca tcgaggcgac gttcgagctc gcgccgatgc agcacgcgtt
    11521 cgtgatggcg ctcgcgctcg acagcgcagc gcgattgacg atgcacgagt ggcaaatgcg
    11581 cctgcggccg tcggtgtggt tcgagcattc tctcgaacgg acgatcgagc tcggactcgt
    11641 gcacacgaca cccgtcgtga ccgctcaccc tcgcctcgtc tcgcgcttgc atggccgcac
    11701 cgtgatcgag atgccgcgcg gcgttcgtta cgaggagctc gaaccgacgc agcgcggact
    11761 gcgccggttt cgtctccacg cgatcggcaa cccgttgccc gaggcgctcc tagaggcggc
    11821 gatcgagatc ggtgtgcacg gcggtgtggt cgcggacgat ggagtatcgt cgccgccatg
    11881 accgccgccg agacgctgct cgccgaagct gtggccgcgg tcaacgctgg cgactacaag
    11941 accgctgcga atcgcttcga acgcgccggt gagctgctca gcgcgaagag cggcaagaag
    12001 ctcggcaaag gccgcgcgcg cgatgctgcg gctgcatacg agtcggcggc gcgttgctac
    12061 ttgatgctga atgatcacaa ccacgcgatt gcagccgtcg agctcgcgcg ccggttgcaa
    12121 cccgcatcac cgcgcgtcgc acgcgtgcac gccgaggtcg cggatcgcgt cggttatgcg
    12181 ccgacgcgcc gccaagcgtg gcaagacgtg ctcaccgctg gtcgtcgcag cgatcggatg
    12241 ttcgcgcagc tgcaactcgc gagcatcgcg cgcgagctcg gtgagcacgc g
//
LOCUS       DASUFX010000218        14939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_867692,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000218 DASUFX010000000
VERSION     DASUFX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867692"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5925
                     /locus_tag="VFV99_08580"
     CDS             <1..5925
                     /locus_tag="VFV99_08580"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX5059400.1"
                     /translation="DRAKLAAAKKIPDAEAEQLLSRARAIKAEATDKQTFALRPMSQP
                     PPRTGNVIKGSFPPPASTLLPPAKSIDNSKDLKVLRYMPEGDVPLAPELSVTFSQPMV
                     AVTSQDDAAATKPVKLVPEPKGRWRWIGTRTVLFDPDVRFPQATTYQVEIPVGTKSAN
                     GGVLKDATKFTFETPAPSIVDRWPSYGPQKLDVPIYALFDQKIDPKAVLAKTKVTANG
                     KPVAIELLDDAAIAKDKTLASIVKAAHESEQDGRWLAFRATEAFPKDAEVRVAFAAGT
                     PSAEGPNVTKGEQSFTFRTFPPLKITDAECGWGGPCRPGMAFQIIFNNPLDEVHFDDA
                     QLSISPDIPGLKIQQSGNQIAISGMTVARTTYKVIVAGGIVDDFAQTLGSDTTLTFSV
                     GDPMPTFYGPQGMVVLDPGAKKPTLDFFTINYEQLKVRLYQVQPSDYDGFGNWMRNRW
                     NHDHPPKLPGKKVFDQLVKTTPGKTSLVETSVDLAPALRNGLGHVIAVVEPYPWKESY
                     EPPTMVAWVQSTKLGIDATVDSDNLLAFATDLASGKPASGVDLEIRPFGIKSKSDEKG
                     LATLGLGTRTIKGAHYLVAKRGDDVAFVSDDGGWWNEYGSWVKQSRGKSLAWYVIDDR
                     KLYKPGEDVSLKGWLRLVDSGKGGDVMGLGGEVSSLSYKVSDSQGREIAKGNATVNQI
                     GAFDTKFTLPKTPNLGYAYIRFETQGRRHESYSHNFQIEEFRRPEFEVNAHASQGPFL
                     VGQSGDVTVDAKYYAGGPLPGAPVNWYLTASQTSYTPPNRDEYVFGQWQPWWGYRSWY
                     DDDGNRYRPAKTWSLEGKTDATGAHVMHLDFLSLNPALPMAVTANASVTDVNRQTWSS
                     AAALIVHPSSLYVGLKTKKPFVEKGHPFDIDVIGVDLDGKAAPGTAIEVKAARIDYEY
                     KKGKYKKVEKDPQTCAVVAAKDAVLCTFQTKQGGSYEVVATIIDSKGRPNQTKLTFWV
                     SGGDQPPARDLAQEKVQLIPDKKEYAGGDTAELLVQAPFYPAEALVTWRRSGIVKVER
                     FAINSSTATIKVPVTDTMVPNLYVQVDLVGMAARTDDNGDPDPKLPKRPAYASGVIDL
                     PVPPKQRTLKVTVAPNAAKLAPGEVARLALDVRDAQGRPVADADTAVIVVDEAVLALT
                     GYQFPSPIDSFYGARDAGARDYYSRSYVKLAKPDAAQLAQSNARGRGPAGATATGGAV
                     REESMDMDKVSYNAPEAPPPPPAPPAKQAAKKGKKEAESRNGKDDEDGENANANTAIA
                     IRSNFNPLAAFSPAVKTDASGKALVEVKLPDNLTRYRIVAIAAAGEKNFGKGESALTA
                     RLPLMVRPSPPRFLNFGDTFKLPVVVQNQTDKPMTVKVAMRATNAAITDGAGREVSVP
                     ANDRVEVLFPAAAEMAGTARFQIVGASGAANDAAELALPVWTPATTEAFATYGVIDDG
                     AVKQPVALPGKVIEQFGGLEVTTASTNLQALTDALLYLVKYPYECAEQRASRILAIAA
                     LRDVLEQFETKDMPSRAALEKSVDEDIERLSQMQNYDGGYAFWDRGYPSNPYLTVFVT
                     SALQHAKDKGFKVQQAMLDRAKPYLKDIESHYPWYYSKEVRWAISSYALYTRKQLGDL
                     DIAKGQKLVKDAGIDKLSMEANGWLLGLFAQNQAASAERKAIVRHALNKVSETAGAAN
                     FTTGYADGNYLLLASDRRVDGVMLESLIAEQPNLDLIPKIVTGLLAHRKAGRWLNTQE
                     NAFVLLALDKYFQTYEKVTPDFVARVWLGKDYAGDHPFKGRQTDSYEIDVPMSFVATH
                     DKQDLTIQKEGKGRLYYRIGMRYAPQSLKLEPADYGFVVQRSYEGADDPKDVTKAQDG
                     TWHIKAGARVRVKLTMVNENRRYHVALVDPMPAGLEPMNPRLATTGPIPTDPNEQKSR
                     GRYWWWYGPWYEHQNMRDERVEAFAALLWEGVHSYEYVARATTPGNFVVPPPKAEEMY
                     MPETFGRGGSDRVIVE"
     gene            5939..12199
                     /locus_tag="VFV99_08585"
     CDS             5939..12199
                     /locus_tag="VFV99_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX5059401.1"
                     /translation="MKNRVLLLVLLATASCRCGKDSTTENKPAATADRLAKAAKDLPD
                     GLDLRLSNGKAGPPPFDKSKLAPAKKLGDADVQQILSRAEALKMEAGDQQDFALRPMS
                     NPPPRTGNTIKDVFPAPPSSLLPPAANDTGKDLRVLRYMPEGEVPLAPELSVTFSQPM
                     VPITSQDDAAKVQPVKLTPQPKGKWRWIGAKTILFDPEVRFPQATTYKVEIPAGTKSL
                     NGGVLKEAVKFTFETPAAKMISHYPGDWSPQHLDVPMFIEFDQKIDPKAVIQKIQVKA
                     PVAPKRAGPSNDPWEKAGTPVMQPVAIRLLDDKEIAKDKPLAAMVEAAKKDEHAGRWL
                     AFRTVQKFSPDAQITVEIPKGTPSLEGPNQTKESQSFSFHTYPPLRVDQADCGWGGEC
                     RPGMALTFVFNNPLDAEKFSEQQVKVSPAIDGMKVIQSGTTVSVVGMTKSRTTYKVTV
                     ASSIVDEFKQTLGKDDTRTFHVGDAVPTFFGPSGLVVLDPSAAKPTLDFFSTNYTGLK
                     VKLYKVQPSDVTAYGRYLREMWNKDHPPQMPGTIVFDKTIPTKVAPNELVETAIDLAP
                     ALNKSGFGHAIAVVEPTPWTEKYDPPRQYAWVESTHIAVDAHVDGENMLVFASDLATG
                     KPLQGVSLEMKPFGITGTSDAQGLATLPLGASERGTHYLLAKKGEDVAFLADDNGSFD
                     EYGSWYKQPREKTVGWFVFDDRKLYKPGEEVSLKGWLRVIDYGKWGDVGAINGAVTSI
                     NYRVVDRTGNEITKGTMPVSPVGGFDTKFTLPKTPNLGYAYVYMNATGRMPTSYTHSF
                     QIEEFRRPEFEVSAQASPGPFVVGGGGDVTVDAKYFAGGALPGAPVNWYVTANQTSFT
                     PPNRDEYVFGSWEPWWGYRAWYDEADPYNPKPYKPPPTWNFAGKTDALGEHILHMDFI
                     SAKPSMPFSVTANATVSDVNRQAWNASTTITVHPSSLYVGVKTKRSFVEKGTPFDIDL
                     IAVDIDGKAQQGAKIEVKTVRLDWEYKNGRYKSKETDPQTCNAVAKTDAVPCEFKTPK
                     GGTYQLTATVVDSKGRANQTKLTYWVGGDDNRPPAREVAQELVQLIPDKKEYTPGNTA
                     ELLVQAPFYPAEGIVTWRRSGIVKTERITLTGPTASLKVPIEDRMTPNLYVQVDLVGM
                     ATRANDRGEPDKALPKRPAYAVGSINLPVPPKQRALQVAVTPEAAKLGPGESTTLAVE
                     VKDAAGRPVADAEAAVVVVDESVLTLAGYSFSDPIGSFYPQRGPDTRDWYQRAYVKLA
                     QPDVSKLAVNLPQGNARGFGGAPGGGAPTTTAIAPAAAPPMEMEADEAPMKKSAEKRM
                     EPAKPMAATKAEAKEDGRYRDDIVAMNGLAKDKNSGLQQGQKPQEPNTPVAIRTNFNP
                     LAAFSPSVKTDANGKATVQVKMPDNLTRYRVVAIAVAGEKQFGKGESAITARLPLMVR
                     PSPPRFLNFGDTFKLPVTVQNQTDQMMTVRLAARTTNLNLTDGQGREVTVPPNDRVEV
                     QFPAAADMAGTARLQVVGVSGTMSDAANLELPVWTPATTEAFATYGVIDDGAIKQQVA
                     LPGKVWPQFGGLEVTTASTNLQALTDAVIYLVHYPFECAEQRSSRILGIASLRDVLTA
                     FKSKDLPSTAAMEHSVDVDIEHLSQMQNSDGGYAYWERGYPSNPYLTVYVTQALLKAK
                     AKRFSINDYMVQRSKYYLQNIENYYDIYYSEDVRNSISAYALYVRKQMGDLDVAKAKA
                     LIARAGGPEKLPMEANGWLLATLAGNASAATERAAIVRHALNKVSETAGAANFTTGYG
                     DGAYLLLASDRRVDAVMLEALIQEQKNLDLIPKLVTGLMAHRKRGRWLNTQENTFVLM
                     ALDLYFKTYEKTTPNFVSRIWLGNDYAGDHAFRGRTTEYYQVGIAMKDVATHDKQDLT
                     IQKDGAGRLYYRIGMKYAPHDLKQFPADYGFAVERRYEAVDDPKDVTQDKDGVWHIKA
                     GARVRVRLNMVNENRRYHVALVDPMPAGLEAMNPALATTGPLPMDPNPKSDAGRGRYW
                     WWYGPWYDHQNMRDERVEAFAPLLWEGNHEYVYIARATTPGNFVVPPPKAEEMYMPET
                     FGRGGSDRVLVE"
     gene            complement(12204..13517)
                     /locus_tag="VFV99_08590"
     CDS             complement(12204..13517)
                     /locus_tag="VFV99_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5059402.1"
                     /translation="MSHDPRTTEALPGSAPQHVLVRECRLTIVGGEHAGRTHAFAAER
                     LVIGADPKADFVIDDGALSKFHCELRIVDGVTYVRDLGSRNGTVIDHLPVIEAPLRDG
                     AVLTLGRTQVRFEVGSREVAVALSPHDHFGKLRGSSIAMRSIYPLLEAAAASASTVLL
                     QGESGTGKDLAAESIHLESARRDGPFVVVDCGAIPAQLLEAELFGHEAGAFTGATSAR
                     TGAFEAAAGGTLLLDEIGELALDLQPKLLRAIDRREIQRIGSTQRIPVDVRVIAATNR
                     DLKAEVNARRFRSDLYFRLAVLVVAMPPLRARTTDIPMLVAAILDTLGDRDSAMARSL
                     AGGELLPELMRHGWPGNVRELRNYVESCIARQELTLTPVPSAEPTIDISEPLRTVRER
                     WLRHVERRYLEELLAAHGGNVSAAARAAGVDRVHMHRLLAKAGLR"
     gene            complement(13514..14713)
                     /locus_tag="VFV99_08595"
     CDS             complement(13514..14713)
                     /locus_tag="VFV99_08595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059403.1"
                     /translation="MRVGNYTLSDLLGRGGTSEVYAGQHQFLGDKVAVKLLRAPLADD
                     ATVRDAFLEEAARTREIQHPNIIRVIDFGHDAISHSCFLVMERIVGESLTARIRRQQI
                     AEPTLREIAARIADGMEAAHARGIVHRDLKPGNIMLRGDVPTIIDFGIAKSLGASTAA
                     HTERRVGTVAYMAPEQLTDGLITPAVDIWALGVILYEAATGELPFANFTDGRLPQLFD
                     TPPRASTHASISRALDDLIVRCLERDPGKRPAAMAEVARALRSADREERLTEDLGAPR
                     VTSRPSPRPATPRRHWPIIAAGVIGGAGIATALVLASQPDRAPSSSATPAMTTTTPPV
                     DAGARATEVPKPSPAALAKPTRAAVEAKPTRHRPKPTVRNDKDKPKTVKQEGEKEKTT
                     SQGETLK"
     gene            14737..>14939
                     /locus_tag="VFV99_08600"
     CDS             14737..>14939
                     /locus_tag="VFV99_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059404.1"
                     /translation="MRWFVVGIVVALQVDAHADPAEDAALVHLDRGVTAFNAKDFHTA
                     HREFTAAHELVPDKANPYRWLALT"
BASE COUNT         2840 a         4783 c         5074 g         2242 t
ORIGIN      
        1 gaccgcgcga agctcgcagc ggcgaagaaa atcccggacg cagaagccga gcagctgctg
       61 tcgcgtgcgc gcgcgatcaa agcggaagcg acggacaagc aaacgttcgc gttgcgcccg
      121 atgtcgcaac cgccgccgcg caccggcaac gtgatcaaag gttcgttccc gccgccggca
      181 tcgacgctgc tcccgcccgc gaagtcgatc gacaacagca aagacctcaa ggtgctgcgc
      241 tacatgcccg agggcgacgt gccgctggcg ccggagctgt ccgtcacgtt ttcgcagccg
      301 atggtcgcgg tgacgtcgca agacgacgcc gctgcgacaa agccggtgaa gctcgtgcca
      361 gagccgaagg gacgctggcg ctggatcggt acgcgcaccg tgttgttcga tcccgacgtt
      421 cgctttccgc aagcgacgac gtatcaggtc gagattccag tcggcacgaa gagtgcgaac
      481 ggcggcgtgc tcaaggacgc gacgaagttc acgttcgaga cgccggcgcc gagcatcgtc
      541 gatcggtggc cgagctacgg gccgcagaag ctcgacgttc cgatctatgc gctgttcgat
      601 cagaagatcg atcccaaggc ggtgctcgcg aagacgaagg tcaccgcgaa cggcaagccg
      661 gtcgcgatcg agctcctcga cgacgccgcg atcgcgaagg acaagacgct cgcgagcatt
      721 gtcaaggcgg cgcacgaatc ggaacaagac gggcgctggc tcgcgttccg cgcgacggaa
      781 gcatttccga aggacgccga ggttcgcgtc gcgtttgcgg cgggcacgcc gagcgccgag
      841 ggtccgaacg tcacgaaggg cgagcagtcg ttcacgttcc gcacgttccc gccgctcaag
      901 atcaccgacg ccgagtgcgg ctggggcgga ccgtgccgtc cgggcatggc gtttcagatc
      961 atcttcaaca acccgctcga cgaggttcac ttcgacgacg cgcagctctc gatctcgccc
     1021 gacattccgg ggctaaagat ccagcagagc ggcaaccaga tcgcgatctc gggcatgacg
     1081 gtcgcgcgca ccacgtacaa ggtgatcgtt gccggcggca tcgtcgatga cttcgcgcaa
     1141 acgctcggca gcgacacgac gctgacgttc tcggtcggcg atccgatgcc gacgttctac
     1201 ggaccgcagg gcatggtcgt cctcgatccg ggcgccaaga agccgaccct cgacttcttc
     1261 acgatcaact acgagcagct caaggtgcgc ctctatcagg tgcagccgag cgactacgac
     1321 gggtttggca actggatgcg caatcgctgg aaccacgatc atccgccgaa gctgcccggc
     1381 aagaaggtgt tcgatcagct ggtcaagacg acaccgggca agacctcgct cgtcgagacc
     1441 agcgtcgatc tggcgcccgc gctgcggaat gggctcgggc acgtgatcgc ggtcgtcgag
     1501 ccgtatccat ggaaagagag ctacgagccg ccgacgatgg tcgcgtgggt gcagtcgacg
     1561 aagctcggca tcgacgcgac ggtcgatagc gacaacttgc tcgcgttcgc gacggacctc
     1621 gcgagtggta agccggcgag cggcgtcgat ctcgagatcc ggccgttcgg catcaagagc
     1681 aagagcgacg agaagggcct cgcgacgctc ggcctcggca cgcggacgat caagggcgcg
     1741 cactatctcg tcgcgaagcg cggcgacgac gtcgcgttcg tctccgacga tggtggctgg
     1801 tggaacgagt acggcagctg ggtgaagcag agccgcggca agtctctcgc ctggtacgtg
     1861 atcgatgatc gcaagctcta caagccgggc gaagacgtgt cgctcaaggg ttggctgcgg
     1921 ctcgtcgaca gcggcaaggg cggcgacgtg atgggccttg ggggcgaggt ctcgtcgctc
     1981 tcgtacaagg tgtccgactc gcagggccgc gagatcgcga agggcaacgc gacggtgaac
     2041 cagatcggcg cgttcgacac caagttcacg ctgccgaaga cgccgaacct cggctacgcg
     2101 tacatccgct tcgagacgca gggccgccgc cacgagagct actcgcacaa ctttcagatc
     2161 gaggagttcc gccgccccga gttcgaggtc aacgcgcacg cgagccaggg cccattcctc
     2221 gtcggccaaa gcggcgatgt caccgtcgac gcgaagtact acgcgggcgg tccgctaccc
     2281 ggtgcgccgg tcaactggta cctgaccgcg agccagacca gctacacgcc accgaaccgc
     2341 gacgagtacg tgttcggcca atggcagccg tggtggggct atcgcagctg gtacgacgac
     2401 gacggcaacc ggtaccgccc cgcgaagacg tggtcgctcg aaggcaagac cgacgcgacc
     2461 ggtgcacacg tgatgcacct cgacttcctg tcgctcaatc ccgcgctgcc gatggcggtg
     2521 actgcaaacg cgagcgtcac cgacgtcaat cgccagacgt ggtcgagcgc ggccgccctg
     2581 atcgtgcatc cgtcgtcgct gtacgtcggc ctcaagacga agaagccgtt cgtcgagaag
     2641 gggcatccgt tcgacatcga cgtgatcggc gtcgacctcg acggcaaggc agcgccgggg
     2701 acggcgatcg aagtgaaggc cgcgcgcatc gactacgagt acaagaaggg caagtacaag
     2761 aaggtcgaga aggatccgca gacgtgcgcg gtcgtcgcag cgaaggatgc cgtgctgtgc
     2821 acgttccaga cgaagcaggg cggcagctac gaggtcgtcg cgacgatcat cgacagcaag
     2881 ggccgaccga accagacgaa gctgacgttc tgggtcagcg gtggcgacca gccgccggcg
     2941 cgcgacctcg cgcaggagaa ggtgcagctg attccggaca agaaggagta cgccggcggc
     3001 gacaccgccg agctgctcgt gcaggcgccg ttctatccgg ccgaggcact cgtcacgtgg
     3061 cggcgcagcg gcatcgtcaa ggtcgagcgc ttcgcgatca attcatcgac cgcgacgatc
     3121 aaggtgccgg tcaccgacac gatggtgccg aacctgtacg tccaggtcga tctcgtcggc
     3181 atggccgcgc gcaccgacga caacggcgat ccggatccga agctcccgaa gcgaccggca
     3241 tacgcatcgg gtgtgatcga tctgccggtg ccgccgaagc agcgcacgct gaaagtcacg
     3301 gtcgcgccga acgccgcgaa gctcgcgccg ggcgaggtgg cgaggctcgc gctcgacgtg
     3361 cgcgatgcgc agggcaggcc ggtcgctgat gccgacacgg cggtgatcgt cgtcgacgag
     3421 gcggtgctcg cgctcaccgg ctatcaattt ccaagcccga tcgattcgtt ctacggtgcg
     3481 cgcgacgcgg gcgcgcgcga ctactactcg cgcagctacg tgaagctcgc gaagccggat
     3541 gcggcgcagc tcgcgcagag caacgctcgt gggcgaggac ccgcaggcgc cactgcaacc
     3601 ggcggcgcgg tgcgcgagga atcgatggac atggacaagg tctcgtacaa cgcgcctgaa
     3661 gcgccaccgc cgccaccggc gccacccgcg aagcaagcgg cgaagaaggg caagaaggaa
     3721 gccgagagcc gcaacggcaa ggacgacgag gacggcgaga acgcgaacgc gaacaccgcg
     3781 atcgcgattc gcagcaactt caacccactc gccgcgttct cgccggcggt gaagaccgac
     3841 gcgagcggca aggcactcgt cgaggtcaag ctgcccgaca acctcactcg ctatcgcatc
     3901 gtcgcgatcg cagcggccgg cgagaagaat ttcggcaagg gcgagagcgc gctcaccgcg
     3961 cggctgccgc tgatggtgcg gccgagcccg ccgcgcttct tgaacttcgg cgacacgttc
     4021 aagctgccgg tcgtcgtcca gaaccagacg gataagccga tgacggtgaa ggtcgcgatg
     4081 cgggcgacga acgcggcgat cacggatggc gcgggtcgcg aggtcagcgt gccggcgaac
     4141 gatcgcgtcg aggtgctgtt cccggcggcc gccgagatgg caggcaccgc gcgattccag
     4201 atcgtcggcg cgagtggcgc tgcgaacgac gcggccgagc tcgcgttgcc ggtgtggacg
     4261 ccggcgacga ccgaagcgtt cgcgacctac ggtgtgatcg acgatggcgc ggtcaagcaa
     4321 cccgtcgcgc tgcccggcaa agtcatcgag cagttcggcg gcctcgaggt aacgacggcg
     4381 tcgacgaatc tgcaggcgct caccgatgcg ctgctctacc tcgtcaagta tccgtacgag
     4441 tgcgcggagc agcgcgcgtc gcgcatcctc gcgatcgcgg cattacgcga cgtgctcgag
     4501 cagttcgaga cgaaggacat gccgtcgcga gcggcgctcg agaaatcggt cgacgaggac
     4561 atcgagcggc tatcgcagat gcagaactac gacggtggct acgcgttctg ggatcgcggg
     4621 tatccgtcga acccgtatct caccgtgttc gtgacgagcg cgctacagca cgcgaaggac
     4681 aaggggttca aggtccagca ggcgatgctc gatcgcgcga agccgtacct caaagatatc
     4741 gagagccact acccctggta ctactccaag gaagttcgct gggcgatcag ctcgtacgcg
     4801 ctctacacgc gcaagcagct cggcgacctc gacatcgcga agggccagaa gctcgtcaag
     4861 gatgcgggca tcgacaagct atcgatggag gcaaacggct ggctgctcgg tttgttcgcg
     4921 cagaaccaag ccgcttccgc agagcgcaag gcgatcgtgc ggcacgcgct caacaaggtg
     4981 agcgagacgg cgggggcggc gaacttcacg accggctacg ccgacggcaa ctacctcttg
     5041 ctcgcgagcg atcgccgcgt cgacggcgtg atgctcgagt cgctgatcgc cgagcagccg
     5101 aatctcgatc tcatcccgaa gatcgtcacc ggcctgctcg cgcatcgcaa agcgggtcgc
     5161 tggctcaaca cgcaagagaa cgcattcgtg ttgctcgcgc tcgacaagta cttccagacc
     5221 tacgagaagg tgacgccgga cttcgtcgcg cgcgtgtggc tcggcaagga ctacgccggt
     5281 gatcatccgt tcaagggccg ccagaccgac agctacgaga tcgatgtgcc gatgtcgttc
     5341 gtcgcgaccc acgacaaaca ggacctcacg attcaaaaag agggtaaggg ccgcctctat
     5401 taccggatcg gcatgcgcta cgcgccgcaa agcctgaagc tcgagccagc ggactacggc
     5461 ttcgtcgttc agcgcagtta cgagggcgcc gacgatccga aggacgtcac gaaagcgcag
     5521 gacggcacct ggcacatcaa agctggcgcg cgcgtcaggg tcaaactcac catggtcaac
     5581 gagaaccggc gctaccacgt ggcgctggtc gatccgatgc ccgcgggact cgagccgatg
     5641 aatccgcggc tcgcgacgac cggaccgatt cccaccgatc cgaacgagca gaagtcgcgc
     5701 ggtcggtatt ggtggtggta cggcccctgg tacgagcacc agaacatgcg cgacgaacgc
     5761 gtcgaagcgt tcgcggcgct gctgtgggaa ggcgtgcact cgtacgaata cgtggcgcgc
     5821 gcgacgacgc ctggaaactt cgtcgtgcct ccgcccaagg cggaggagat gtacatgccc
     5881 gagacgttcg gtcggggtgg cagtgatcga gtcatcgtcg aatgagagga gctagctagt
     5941 gaaaaaccgc gtactactgc tcgtactgct ggctactgcg tcttgcagat gcggcaagga
     6001 ctcgacgacc gagaacaagc ctgccgccac cgcggacagg ctcgcgaagg cggcaaagga
     6061 tctgccggac gggctcgacc tgcgcctctc gaacggcaag gcggggccgc caccgttcga
     6121 caagagcaag ctcgcgccgg cgaagaagct cggcgacgcc gacgtccagc agatcttgtc
     6181 gcgcgccgag gcgctgaaga tggaggcggg cgatcagcag gatttcgcgc tgcgcccgat
     6241 gtcgaatccg ccgccgcgca ccggcaacac gatcaaggac gtgttcccgg cgccgccgtc
     6301 gtcgctgttg cctccggccg cgaatgacac cggcaaggat ctgcgcgtgc tgcgctacat
     6361 gccggaaggt gaggtgccgc tcgcgcccga gctctcggtg acgttcagcc agccgatggt
     6421 gccgatcacc tcgcaggacg acgcggcgaa ggtgcagccg gtgaagctga cgccgcagcc
     6481 gaagggcaag tggcgctgga tcggcgcgaa gacgatcctg ttcgatcccg aggttaggtt
     6541 cccgcaagcg acgacgtaca aggtcgagat tccggcgggc acgaagagcc tgaacggcgg
     6601 cgtgctgaag gaggccgtga agttcacgtt cgagacgccg gccgcgaaga tgatctcgca
     6661 ctaccccggc gactggtcac cgcagcacct cgacgtgccg atgttcatcg agttcgatca
     6721 gaagatcgat ccgaaggccg tcatccagaa gatccaggtc aaagcgccgg tcgcgccgaa
     6781 gcgcgctggg ccgtcgaacg atccatggga gaaggcgggc acgccggtga tgcagccggt
     6841 cgcgattcgg ctgctcgatg acaaggagat cgcgaaggac aagccgctcg ccgcaatggt
     6901 cgaggcggcg aagaaggacg agcatgccgg tcgttggctc gcattccgca ccgtgcagaa
     6961 gttctcgccc gacgcgcaga tcaccgtcga aattccgaaa ggcacaccga gcctcgaagg
     7021 tccaaaccag accaaggagt cgcagtcgtt ctcgttccac acctatccgc cgctgcgcgt
     7081 cgatcaagcg gactgcggct ggggcggtga gtgccggccc ggcatggcgc tgacgttcgt
     7141 gttcaacaac ccgctcgacg ccgagaagtt cagcgagcag caggtcaagg tgtcgccggc
     7201 gatcgacggc atgaaagtga tccagagcgg cacgaccgtc tcggtcgtcg gcatgacgaa
     7261 gtcgcgcacg acgtacaagg tcacggtcgc gagctcgatc gtcgacgagt tcaagcagac
     7321 gctcggcaag gacgataccc gcacgttcca tgtcggcgac gccgtcccga cgttctttgg
     7381 accgtcgggg ctcgtcgtgc tcgatccgtc ggcggcgaaa ccgacgctcg acttcttctc
     7441 gacgaactac accggcctca aggtgaagct ctacaaggtg cagccgagcg acgtcaccgc
     7501 gtacggccgc tatctgcgcg agatgtggaa caaggatcat ccgccgcaga tgccgggcac
     7561 gatcgtgttc gacaagacga tcccgacgaa ggtcgcgccg aacgagctcg tcgagaccgc
     7621 gatcgatctc gcacccgcgc tcaacaagtc tgggttcggt cacgcgatcg ccgtcgtcga
     7681 gccgacgccg tggaccgaga agtacgatcc gccgcgccaa tacgcgtggg tcgagtcgac
     7741 gcacattgca gtcgacgcgc acgtcgatgg cgagaacatg ctcgtgttcg cgagcgacct
     7801 cgcgaccggc aagccgctcc aaggcgtgtc gctcgagatg aagccgttcg gcatcacggg
     7861 cacgagtgat gcgcagggcc tcgccacgct gccgctcggt gcgagcgagc gcggcacgca
     7921 ttacttgctc gcgaagaagg gcgaggacgt cgcgttcctc gccgacgaca acgggtcctt
     7981 cgacgagtac ggcagctggt acaagcagcc acgcgagaag accgtcggct ggttcgtgtt
     8041 cgacgatcgc aagctctaca agccgggcga ggaagtttcg ctcaagggct ggctgcgcgt
     8101 catcgactac ggcaagtggg gcgacgtcgg cgcgatcaac ggtgcggtca ccagcatcaa
     8161 ctatcgcgtc gtcgatcgca ccggtaacga gatcacgaag ggcacgatgc cggtgtcgcc
     8221 ggtcggtggc ttcgacacca agttcacgct gccgaagaca ccgaacctcg gctacgcgta
     8281 cgtgtacatg aacgcgaccg gccgcatgcc gacgtcgtat acgcattcgt tccagatcga
     8341 agagttccgc cggcccgagt tcgaggtgtc ggcgcaggcg agcccgggac cgttcgtcgt
     8401 tggcggcgga ggcgatgtca ccgtcgacgc gaagtacttc gcgggcggtg cgttgcccgg
     8461 cgcgccggtc aactggtacg tgaccgcgaa ccagaccagc ttcacgccgc cgaaccgcga
     8521 cgagtacgtg ttcggtagct gggagccgtg gtggggctac cgcgcctggt acgacgaggc
     8581 cgatccgtac aacccgaagc cgtacaagcc accgccgacg tggaacttcg cgggcaagac
     8641 cgatgcgctc ggcgagcaca tcctccacat ggacttcatc agcgcgaagc cgtcgatgcc
     8701 gttctcggtc accgcgaatg cgacggtaag tgacgtcaat cgccaggcgt ggaatgcgtc
     8761 gacgacgatc accgtacatc cgtcgtcgct ctacgtcggc gtcaagacga agcgctcgtt
     8821 cgtcgagaag ggcacgccgt tcgacatcga tctgatcgcg gtcgacatcg acggcaaggc
     8881 gcagcagggc gcgaagatcg aagtgaagac ggtgcggctc gactgggagt acaagaacgg
     8941 ccgctacaag tcgaaggaga ccgatccgca gacgtgtaac gcggtcgcga agaccgacgc
     9001 cgtgccgtgc gaattcaaga cgccgaaggg cggcacctat cagctgaccg cgacggtcgt
     9061 cgacagcaag ggccgcgcga accagacgaa gctgacgtac tgggtcggcg gcgacgacaa
     9121 ccggccgccc gcgcgtgaag tcgcgcagga gctcgtgcag ctgattcccg acaagaagga
     9181 gtacacgccg ggcaacacgg cggagctgct cgtgcaggcg ccgttctatc cggccgaagg
     9241 catcgtgacg tggcggcgca gcggcatcgt gaagaccgag cgcatcacgc tcaccggtcc
     9301 gacggcgtcg ctcaaggtgc cgatcgagga tcggatgacg ccgaacctgt acgtgcaggt
     9361 cgacctcgtc ggcatggcga cgcgcgcgaa cgatcggggc gagccggata aggcgctgcc
     9421 gaaacgtccc gcgtatgcgg tcggctcgat caacctgccg gtgccgccga agcagcgcgc
     9481 gctgcaagtc gcggtcacgc cggaggcggc gaagctcggt cccggtgaat cgacaacgct
     9541 cgcagtcgaa gtgaaggacg cagccggcag gccggtcgct gatgcggaag cggcggtcgt
     9601 cgtcgtcgac gaatcggtgc tcacgctcgc cggttacagc ttctccgatc cgatcggctc
     9661 gttctatccg cagcgcggcc ccgacacgcg tgactggtat cagcgcgcat acgtcaagct
     9721 cgcgcagccc gacgtctcga agctcgcggt caatctgccg caaggcaatg ccaggggctt
     9781 tggcggcgcg ccgggtgggg gtgctccgac gacgaccgcg attgcgccgg ccgcggcacc
     9841 gccgatggag atggaggccg atgaagcgcc gatgaagaaa tcggcggaga agcgcatgga
     9901 accggcgaag ccgatggcgg cgacgaaggc cgaggcgaaa gaggacggcc gctaccgcga
     9961 cgacatcgtg gcgatgaacg ggctcgccaa ggacaagaac agcggtctcc agcaaggcca
    10021 gaagccacaa gagccgaaca cgccggtcgc gatccgcacg aacttcaacc cgctcgcggc
    10081 gttctcgccg tcggtgaaga cagacgcgaa tggtaaggcg accgtgcagg tgaagatgcc
    10141 cgacaacctg acgcgctatc gcgtcgtcgc gatcgcggtc gccggtgaga agcagttcgg
    10201 aaagggggag agtgcgatca ccgcgcggtt gccgctgatg gtgcggccga gcccgccgcg
    10261 gttcttgaac ttcggcgaca cgttcaagct gccggtcaca gtccagaacc agaccgacca
    10321 gatgatgacg gtgcggctcg ccgcgcggac gacgaacttg aacctcaccg acggtcaggg
    10381 tcgcgaggtg acggtgccgc cgaacgatcg cgtcgaggtg cagttccccg cggccgccga
    10441 catggcgggc acggcgcgac tgcaggtcgt cggcgtatcg gggacgatga gcgacgcggc
    10501 aaacctcgag ttgccggtgt ggacgccggc gacaaccgag gcgttcgcga cgtacggcgt
    10561 gatcgacgac ggcgccatca agcagcaggt cgcgctgcct ggcaaggtgt ggccgcagtt
    10621 cggtggcctc gaggtcacga ccgcgtcgac gaacctgcag gcgctcaccg atgcggtgat
    10681 ctacctcgtg cactatccgt tcgaatgcgc ggagcagcgc agctcgcgca tcctcggcat
    10741 cgcgtcgttg cgcgacgtgc ttaccgcgtt caagagcaag gatctgccga gcacggcggc
    10801 gatggagcat tcggtcgacg tcgacatcga gcacctgtcg caaatgcaga actccgacgg
    10861 tggctacgcg tactgggagc gcggctatcc gtcgaatccg tacctcacgg tgtacgtgac
    10921 gcaggcgctg ctcaaggcca aggcgaagcg gttctcgatc aacgactaca tggtccagcg
    10981 gtcgaagtac tacctgcaga acatcgagaa ctactacgac atctattact ccgaggatgt
    11041 tcgcaactcg atcagcgcgt acgcgctcta cgtgcgcaag cagatgggtg acctcgacgt
    11101 cgcgaaggcg aaggcgctga tcgcgcgcgc cggcggcccc gagaagctgc cgatggaagc
    11161 gaacggttgg ctactcgcca cgctcgccgg taacgcgagt gcggcgaccg agcgcgcggc
    11221 aatcgtgcgt cacgcactca acaaggtcag cgagaccgca ggcgcggcga acttcacgac
    11281 cggctacggc gacggcgcgt atctgctgct cgcgagtgat cggcgcgtcg acgcggtgat
    11341 gctcgaagcg ctgatccagg aacagaagaa tctcgatctg attccgaagc tcgtcaccgg
    11401 cctgatggcg cacagaaagc gcggccgctg gctcaacacg caagagaaca cgttcgtgct
    11461 gatggcgctc gacctctact tcaagacgta cgagaagacg acgcccaact tcgtgtcgcg
    11521 catctggctc ggcaacgact acgccggcga tcacgcgttc cgcggtcgca cgacggagta
    11581 ctaccaggtc ggtatcgcga tgaaggacgt cgcgacgcac gacaagcagg accttacgat
    11641 ccagaaggac ggcgcgggcc gcctctacta ccgcatcggc atgaagtacg caccgcacga
    11701 cctcaagcag ttcccggcgg actacggctt cgccgtcgag cggcgctacg aagcggtcga
    11761 cgatccgaag gacgtcacgc aggacaagga cggtgtgtgg cacatcaagg cgggtgcgcg
    11821 cgtgcgcgtg cggctcaaca tggtcaacga gaaccggcgc tatcacgtcg cgctcgtcga
    11881 tccgatgccg gccggcctcg aggcgatgaa ccccgcgctc gcgaccactg gtccgttgcc
    11941 gatggatccg aatccgaagt cggatgcggg gcgcggccgg tactggtggt ggtacgggcc
    12001 ttggtacgat caccagaaca tgcgtgacga gcgcgtcgaa gcgttcgcgc cgctgctctg
    12061 ggaaggcaac cacgagtacg tgtacatcgc gcgcgcgacg acgccgggta acttcgtggt
    12121 gccgccgccg aaggcagaag agatgtacat gcccgagacg ttcggccgcg gcggcagcga
    12181 tcgcgtgctc gtcgagtgac gcgctagcgt aggccggctt tggcgagcag gcgatgcatg
    12241 tgcacgcggt cgacgccggc cgcgcgcgca gcggcgctga cgttgccgcc gtgggcggcg
    12301 agcagctcct cgaggtagcg gcgctcgacg tgacgcagcc agcgctcgcg taccgtgcgc
    12361 agcggctccg agatgtcgat cgtcggttcc gcggacggca ccggggtgag cgtgagctcc
    12421 tgacgtgcga tgcacgactc gacgtagttg cgcagctcgc gcacgttgcc gggccagcca
    12481 tggcgcatca gctcgggaag cagctcgccg cctgcgagcg agcgcgccat cgccgagtcg
    12541 cgatcgccga gcgtgtcgag gatcgcggcg accagcatcg ggatgtcggt cgtacgcgcg
    12601 cgcagcggcg gcatcgcgac gacgagcacg gcgagccgga agtagaggtc ggagcggaac
    12661 cggcgcgcgt tgacctcggc tttgaggtcg cggttcgtcg cggcgatcac gcgcacgtcg
    12721 acgggaattc gctgcgtgct gccgatgcgc tggatctcgc ggcgatcgat cgcgcggagc
    12781 agcttcggct gcaggtcgag tgcgagctcg ccgatctcgt cgagcagcag cgtgccgccg
    12841 gcggccgctt cgaatgcgcc ggtgcgcgcc gacgttgcac cggtgaacgc gccggcttcg
    12901 tgaccgaaca gctcggcctc gagtagctgc gcggggatcg caccgcagtc gacgacgaca
    12961 aacggcccat cgcggcgagc gctctcgaga tggatcgatt ccgcggcgag atccttgccg
    13021 gtgccgctct cgccttgcag cagcacggtg ctcgccgatg cagcggccgc ttcgagcagc
    13081 ggatagatcg accgcatcgc gatcgagctg ccgcgtagct tgccgaagtg atcgtgtggc
    13141 gacagcgcga ccgcgacctc gcgcgagccg acctcgaagc gtacttgcgt gcggccgagc
    13201 gtgagcaccg cgccatcgcg cagtggcgct tcgatcacag gcaggtgatc gatcaccgta
    13261 ccgttacggc ttcctaggtc gcgcacgtac gttacgccgt cgacgatgcg cagctcgcaa
    13321 tggaacttcg agagcgcgcc atcgtcgatc acgaagtccg ctttcggatc cgcgccgatc
    13381 acgagccgct ccgcggcgaa cgcgtgtgtg cgaccggcgt gctcgccgcc gacgatcgtc
    13441 agccggcact cgcggaccag cacgtgctgc ggagcagagc cgggcagggc ctcggtggta
    13501 cgcgggtcgt ggctcatttc agcgtttctc cctggctcgt cgtcttctcc ttctcgccct
    13561 cctgcttcac ggtcttcggc ttgtctttgt cgtttcgcac cgtcggcttc ggccggtggc
    13621 gcgttggctt cgcctcgacg gccgcgcgcg tcggcttcgc tagggcggca ggtgatggtt
    13681 tgggcacctc cgtcgcgcga gcgccggcat cgacgggggg cgtggtcgtc gtcatggcag
    13741 gagtcgcgga cgacgaaggc gcacgatcgg gttgactcgc aagtacgagc gccgtcgcga
    13801 tgcctgcccc accgatgacc cctgcggcga tgatcggcca gtgtcggcgg ggcgtcgcgg
    13861 ggcgcggtga cggtcgcgac gtcacgcgtg gcgcgccgag gtcttcggtc aggcgctctt
    13921 cacgatcggc gctccgcagc gcgcgtgcga cctcggccat cgcggcgggc cgcttgcccg
    13981 gatcgcgctc gaggcatcgc acgatcagat cgtcgagcgc acgtgagatc gatgcgtgcg
    14041 tcgacgcgcg tggcggtgtg tcgaacagct gcggcaacct gccgtcggtg aagttcgcga
    14101 acggcagctc gccggtcgcg gcctcgtaaa ggatcacgcc gagcgcccag atatcgacgg
    14161 cgggtgtgat gagcccatcc gtcaattgct ccggcgccat gtacgcgacc gtcccgacgc
    14221 ggcgctcggt gtgcgccgcc gtggacgcgc cgaggctctt cgcgatcccg aagtcgatga
    14281 tcgtcggcac gtcaccgcgc agcatgatgt tcccgggctt gagatctcgg tgaacgatgc
    14341 cgcgcgcgtg tgccgcctcc atgccgtccg cgatgcgcgc tgcgatctcg cgcagcgtag
    14401 gctccgcgat ctgctggcgc cggatccgcg ctgtaagcga ctcgccaacg atgcgctcca
    14461 tcacgaggaa gcaggagtgg ctgatcgcgt cgtgaccgaa gtcgatcacg cggatgatgt
    14521 tcgggtgctg gatctcgcgc gtgcgcgccg cctcctccag gaacgcatcg cgcacggtcg
    14581 cgtcatcggc gagcggcgcc cgcaacagct tcacggcgac tttgtcgcca agaaactggt
    14641 gctgcccggc gtacacctcg ctcgtgccgc cgcggccaag cagatccgag agcgtgtaat
    14701 tgccgacgcg caacgtgcgc gttatcgtcg cacgagatgc ggtggttcgt cgtcgggatc
    14761 gtcgtcgcgc tgcaagtcga cgcacatgcc gatccagccg aggatgcagc gctcgtacac
    14821 ctcgatcgcg gcgtcactgc tttcaacgcc aaggattttc acaccgccca ccgcgagttc
    14881 acggcggcgc acgagctcgt gcccgacaag gcgaatccct accgctggct cgcgctcac
//
LOCUS       DASUFX010000219         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_588476,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000219 DASUFX010000000
VERSION     DASUFX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588476"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VFV99_08605"
     CDS             complement(<1..1339)
                     /locus_tag="VFV99_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5059405.1"
                     /translation="MIAALIRWSIANRFLVLLATAIVAAWGVWSLARTPLDALPDLSD
                     VQVIIRTTYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQSRLPAEAKASLGPDATGVGWVFQYALVDRTGGHDLAQLRALQ
                     DWFLKYELKTVPNVAEVASVGGMVKQYQIVLDPDRLRAYNLPHGDIVEAIERANQESG
                     GSVIELGEAEYMVRASGYLQSLDDFRKIPLMTTSAGVSVRLGDVARVQVGPEMRRGIS
                     ELDGEGEATGGVIVMRTGQNALETIDAVKAKLEELQASLPKGVEIVPIYDRSSLIERA
                     IDNLTKKLLEEFIVVAVVCFIFLFHLRSAFVAIVSLPLGVLASFIVMRYQGVNANIMS
                     LGGIAIAIGAMVDAAVVMIENAHKHLERWRHDHASATLDAETRWRVIGDAAAEV"
     gene            complement(1336..2874)
                     /locus_tag="VFV99_08610"
     CDS             complement(1336..2874)
                     /locus_tag="VFV99_08610"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5059406.1"
                     /translation="MRRPLIFMIGVAAIAALALGGYALYRLGMSHGMDMAATAPSDSG
                     ARPRESASLKAGDIDPATGKRVLYWHDPMVPGRRFDQPGKSPFMNMQLVPVYEGGNGA
                     DGGVTVSARVQQNLGMRTAEVVRTTMAPRIEAVGNIAFNERDQAVVQARAMAYVEKLH
                     VRATLDRVAAGAPLADLYVPEWVAAQEEFLSVLRMRGTELAVLVDGARQRMRQVGMSD
                     EQIRRVEELGQVQPRIALRAPLAGVIVELEAREGMTVMPGDTLFRINGLSTVWANAAV
                     PESQAALLRPGAPVEARSAAIPDTVFAGTVQAILPDVDPDTRTIKARVELANPDFELA
                     PGMFVSVALAGPATDTLIVPTEAVIETGRRTVVMLAESNGTFQPVDVEIGIESNGQTE
                     IKRGLSVGQMVVVSGQFLIDSEASLRATSTRMEDRPPMNEPTTEHFGEGKVESLDDES
                     VTLSHAPIPSVPWPAMTMRFALPPGGPLHGVEVGDDVRFAFAMGGDGNPRITRIEPIA
                     ESPQ"
     gene            complement(2895..4187)
                     /locus_tag="VFV99_08615"
     CDS             complement(2895..4187)
                     /locus_tag="VFV99_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5059407.1"
                     /translation="MPHAFFTVRTVCPLVATALLLGAPAPLDAQNAAALTIDEALQLA
                     AQRSQQLPAQDAAAAAAREMATAAAQGPDPTLTAGINNLPINGPDAFSLTRDFMTMRS
                     IGLMRELTRGDKLEARSARFEREAEAADARRTLALTDLQRGTAMAWFDRYYRERVRDV
                     LALEREEAALQIEAADLAFRTGLGPQSDVFAARSSVAEIEDRIAANERDGEIATIKLA
                     RWIGDAAARPLADPPATDTVSLHPADLDTQLAHHPQIQLMLKQEEMARADAEVARTNK
                     RSDWTVEVMYSQRGPEFSNMMSLNVSKPLQWRERNRQDRELGAKLAMAEEMRAEREEE
                     TRVHVAEAHAVLQAWQRNRERLDRYTSTLIPLAGERTLAATAAYRGGTGTLDAVLEAR
                     VDEIDTRLDHLMLEMETAELWAEINYLIPVGHDAGAHE"
     gene            complement(4263..>4628)
                     /locus_tag="VFV99_08620"
     CDS             complement(4263..>4628)
                     /locus_tag="VFV99_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059408.1"
                     /translation="MISRLSRVLILLAAAGVAYAGPVAACLCVDGPADEMPCCPDDVQ
                     QQDHSNCPQPDSEVAVACDTVPADLLPAGTQDLSPPVAISAALPPWAVHGPPVWSITT
                     SPPHHHSPPIYLVTLRLRI"
BASE COUNT          747 a         1551 c         1538 g          792 t
ORIGIN      
        1 cgacctcggc ggctgcgtcg ccgatcacgc gccagcgcgt ttcggcgtcg agtgttgcac
       61 tcgcatgatc gtgccgccac cgctcgaggt gcttgtgagc gttctcgatc atgacgaccg
      121 cggcgtcgac catcgcgccg atcgcgatcg cgatcccgcc gagcgacatg atgttcgcgt
      181 tgaccccctg atagcgcatc acgatgaagc tcgcgagcac gccgagcggc agcgagacga
      241 tcgccacgaa cgccgagcgc aggtggaaca agaagatgaa gcagacgacg gcgacgacga
      301 tgaattcctc taggagcttt ttcgtcaaat tgtcgatcgc ccgctcgatc aggctcgaac
      361 gatcgtagat cggcacgatc tcgacgcctt tcggcaagct cgcctgaagc tcctcgagct
      421 tcgccttgac ggcgtcgatc gtctcgagcg cgttctggcc ggtccgcatc acaatcacgc
      481 cgccggtcgc ctcgccctcg ccgtcgagct cgctgatgcc gcggcgcatc tcggggccga
      541 cctgcacccg ggcgacgtcg ccgagccgga cggacacgcc ggcgctcgtc gtcatgagcg
      601 gaatcttccg gaagtcgtcg agcgactgga ggtaccccga ggcacgcacc atgtattccg
      661 cttcgccgag ctcgatgacc gagccgccgc tctcttggtt cgcgcgctcg atcgcctcga
      721 cgatgtcgcc gtgcggaagg ttgtacgctc gcagccggtc gggatcgagc acgatctgat
      781 attgcttgac catgccgccg acgctcgcga cctcggcgac gttcggcacg gtcttgagct
      841 cgtatttgag gaaccagtcc tgaagcgcgc gcagctgggc gagatcgtgg ccgccggtgc
      901 ggtcgacgag cgcgtactga aagacccagc cgacgccggt cgcatcggga ccaagcgacg
      961 ccttcgcttc ggccggcagg cgcgactgca cttggttgag gtactcgagc acgcgcgagc
     1021 gcgcccaata gagatccgtg ccgtcctcga ataagacgta cacgaacgag tcgccgaaga
     1081 atgagaagcc gcggactgtc ttcgccccgg gcaccgacag catcgtcgtc gtgagcggat
     1141 aagtgacctg attctcgacg atctgcggcg cttgaccggg ataggtcgtc cggatgatca
     1201 cctgcacgtc ggagagatcg ggcagggcgt cgagcggcgt ccgcgcgagc gaccagacgc
     1261 cccatgcggc gacgatcgcg gtcgcgagca gcacgaggaa gcgattcgcg atcgaccaac
     1321 ggatcagcgc ggcgatcatt gcggactctc cgcgatcggt tcgattcggg tgatccgcgg
     1381 gttgccgtcg ccgcccatcg cgaacgcgaa gcgcacgtcg tcgccgactt cgacgccgtg
     1441 cagcggaccg cccggcggga gcgcgaaccg catcgtcatc gcgggccacg ggaccgacgg
     1501 aatcggtgcg tgcgagagcg tcacgctctc gtcgtcgagc gattcgacct tgccctcacc
     1561 gaagtgctcg gtcgttggct cgttcatggg cggcctgtct tccatgcgcg tgctcgtcgc
     1621 gcggaggctt gcctcggagt cgatcaggaa ctgccccgac accacgacca tttgtccgac
     1681 gcttaagccc cgtttgatct cggtttggcc gttcgactcg atgccgatct cgacgtcgac
     1741 cggctgaaac gtgccattcg attccgcgag cattacgacc gtccggcggc ccgtctcgat
     1801 cacggcttcg gtcggcacaa tcaacgtatc cgttgcaggt ccggcgagcg cgacactcac
     1861 gaacatgccg ggcgcaagct cgaagtccgg attcgcgagc tcgacgcgag ccttgatcgt
     1921 acgcgtgtcg ggatcgacgt cgggcaggat cgcctgcacg gtgccggcaa aaacggtgtc
     1981 tggaatcgcg gcggagcgcg cttcgacggg agctccgggc cggagcaacg cggcctggct
     2041 ctcgggcact gcggcattcg cccacaccgt cgagagcccg ttgatccgaa acagcgtgtc
     2101 gcctggcatc accgtcatgc cttcgcgcgc ctcgagctcg acgatcacgc cggcgagcgg
     2161 cgcgcgcagc gcgatccgcg gttgcacctg gccgagctcc tcgacgcggc gtatttgctc
     2221 gtcgctcatg ccgacttggc gcatccgttg ccgcgcgccg tcgacgagca cggcgagctc
     2281 cgtgccgcgc atgcgcagca cggagagaaa ctcctcctgc gctgcgaccc attcggggac
     2341 gtagagatcg gcgagcgggg cccccgcggc gacgcgatcg agcgtcgcgc gcacgtgcag
     2401 tttctcgacg taggccatcg cgcgcgcctg cacgaccgct tgatcgcgct cattgaacgc
     2461 aatgttgccg accgcttcga tgcgcggcgc catcgtcgtg cgcacgactt cggcagtgcg
     2521 catacctagg ttctgctgca cgcgggcgct cacggtgacg ccgccgtcgg cgccgttgcc
     2581 gccctcgtac acgggcacga gctgcatgtt catgaacggc gatttgccgg gctgatcgaa
     2641 gcggcgtccc ggtaccatcg ggtcgtgcca gtagagcact cgcttcccgg tggcgggatc
     2701 gatgtcgccg gccttgagcg atgcgctttc gcgcggccgg gcacccgagt cggacggcgc
     2761 ggtcgcagcc atgtccatac cgtgcgacat accgagccgg tacaacgcgt agccgccgag
     2821 cgcaagcgcc gcgatcgcgg ctacgccgat catgaagata agtggacgtc tcatcgctgc
     2881 taggtccttg tgcatcactc gtgcgcgcct gcgtcgtgac cgacggggat caaatagttg
     2941 atctcggccc agagctctgc ggtctccatc tcgagcatca agtgatcgag acgcgtgtcg
     3001 atctcgtcga cgcgcgcctc caagacggcg tcgagcgttc ccgtgccgcc gcgatacgct
     3061 gcggttgccg cgagcgtgcg ttcgccggcg agagggatca gcgtgctcgt gtagcggtcg
     3121 agcctctcgc ggttgcgctg ccacgcctgg agcactgcgt gcgcttcggc gacgtgcacg
     3181 cgagtctcct cttcgcgctc ggcgcgcatc tcttcggcca tcgccaactt cgccccgagc
     3241 tcgcggtcct ggcggttccg ctcgcgccac tgcaacggct tcgacacgtt caacgacatc
     3301 atgttggaga actccggtcc tcgctggctg tacatgactt cgacggtcca atccgaacgt
     3361 ttgttcgtgc gcgcaacctc ggcgtcggcg cgcgccatct cctcttgctt gagcatcagt
     3421 tggatctgcg gatggtgcgc gagctgagtg tcgagatccg cggggtggag cgaaaccgta
     3481 tcggtggccg gcggatcggc gagcggtcgc gccgcggcgt cgccgatcca gcgcgcgagc
     3541 ttgatcgtgg cgatttcgcc atcgcgctcg ttcgcggcga tccgatcttc gatttcggcc
     3601 accgacgagc gggccgcgaa cacgtcgctc tgtggcccca agcccgtgcg aaacgcgaga
     3661 tcggcggctt cgatctggag cgccgcttcc tccctctcga gtgcgagcac gtcgcgtacg
     3721 cgctcccgat agtaccgatc gaaccaagcc atcgcggtgc cgcgctgaag atcggtgagc
     3781 gcgagcgtac ggcgggcgtc cgcggcctcc gcctcgcgct cgaagcgtgc ggaacgcgcc
     3841 tcgagcttgt cgccgcgcgt gagctcgcgc atcaagccga tcgagcgcat cgtcatgaaa
     3901 tcgcgcgtca ggctgaacgc gtccgggccg ttgatcggca ggttgttgat tccggcggtg
     3961 agcgtagggt ccggcccctg cgcggccgcc gttgccatct cgcgcgccgc cgccgctgcc
     4021 gcatcctgtg ccggcaactg ctgcgatcgc tgcgccgcga gttgcagagc ttcatcgatc
     4081 gtcagcgcag cggcattctg cgcatcaagc ggcgcggggg cgccgagcag cagcgccgtc
     4141 gcaacgagcg gacataccgt acgaacagtg aaaaacgcgt gaggcacgac cttctccacg
     4201 gctgttcttg gaaacaggca tagggctcac ctgaggattc acctcaggcg cgaggtcggc
     4261 gcttaaatcc gcagtcgcag cgtgacgaga tagatcggcg gggaatgatg gtggggcggt
     4321 gaggtcgtga tggaccacac cggcgggccg tgcaccgccc acggcggcag cgccgccgag
     4381 atcgcgaccg gcggcgacag gtcctgcgtg ccggccggga gcaagtcggc cggcaccgtg
     4441 tcgcaggcga cggccacttc ggaatcgggc tgtggacagt tggaatgatc ctgttgctgc
     4501 acgtcgtccg gacagcacgg catctcgtcg gctggcccgt cgacgcacaa acacgcggcg
     4561 accgggccgg cgtacgcgac gccggccgcg gcaagaagaa tcaatacacg cgagagccgc
     4621 gagatcat
//
LOCUS       DASUFX010000220        22984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1144634, whole genome shotgun sequence.
ACCESSION   DASUFX010000220 DASUFX010000000
VERSION     DASUFX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22984
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144634"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFV99_08625"
     CDS             complement(<1..450)
                     /locus_tag="VFV99_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059409.1"
                     /translation="MGPPSDIERLIELGLNRYGAGDLDGALLMWEEALAIDPDNARAS
                     SYVDYVRLHYELLSSEPHVEGAEEAPFGIEEEPEYQIEILPGDLPQRESQPVLANDEV
                     DAGWFDEEATHDINVHARRSSGEIEIEADAPPRRSSAQIEIEAEAPPL"
     gene            complement(457..2289)
                     /gene="dnaK"
                     /locus_tag="VFV99_08630"
     CDS             complement(457..2289)
                     /gene="dnaK"
                     /locus_tag="VFV99_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5059410.1"
                     /translation="MASEKVIGIDLGTTNSCVAIVEDGTPSVIPNRGGYKTTPSMVAI
                     AENGKRLVGHIAKRQAITNAENTVYAAKRLIGRKWGSAAVRSSLEIMPYRIVEGPHDD
                     VRIMLRDQVFSVPEVSAYILQEMKLISEEYLGEEVQKAVITVPAYFNDGQRQATKDAG
                     RIAGLDVIRIINEPTAAALAYGFGRNIERKVAVYDLGGGTFDISILEIGNGVFEVIST
                     AGDTFLGGEDFDRRIIDWLVFGFEKQHGIDLRKDKMALQRLKDVAEKAKCELSSVKET
                     EINLPFIISTGRNEALHLQTMLTRDKLEELTSDLVERTVEICARTLEEAEIQKNQIED
                     VILVGGMTRMPRVQQACAEFFGLEPCKGVHPDEVVALGAAIQGAALTDEKSDILLLDV
                     TPHSLGIMIVGGYFHKLIEQNTTVPTSESHVFTTVKDNQTSVKILVLQGESDRAEENE
                     LLGEFVLTGLRRAPRGEVEIEVQFHISADGIVSVKAKDLETGLEQSITVTATSGLTED
                     EIKKMIDANKDYMVEVRTGQEFDKARHQAEKSLEEIESLFPQVTDAIAGSDFGQDAVK
                     KARAVVERTRQAITAKDLTTLADATEALNRTLNMFRGVVSKTRG"
     gene            complement(2373..3800)
                     /locus_tag="VFV99_08635"
     CDS             complement(2373..3800)
                     /locus_tag="VFV99_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5059411.1"
                     /translation="MSSVPSEIVGLGSSSMKLRVSPGFDPLKAQVGPDEYFVLSRIDG
                     TQSIREILLATGLPVDRGIAIVTKLRSIGALLLPGETAAPTPMSVPARGTGPRPNSTT
                     PVAPLTPPHGVPSPVAEQMGRAPTVRVTPHEMPAVLPPRTITPHLPVTGRTPMRPGTG
                     ERPTVPRTMTPHLPMDAKRPAPPSRPPADPPTQPRTQLGTHPAAGMRPPAPESVDPPT
                     VRRASTAELHLMLPQPTAEEKSALAEPGDLDADERVRILVMARLVDERDPWALLGVPT
                     GSDAKVLKRAYFTLSKEIHPDRHYGKQLGTFADRLSVVFEAVSRAYARLTSPAKDQRS
                     GAYPMVQTEQPQTPGEYAGELFQRACGLEVGGDALGAMKLFAAAVRIDPQTRYLRRAA
                     TCALAAEQPKTALEYAKKAQAQAPNDPSSARLLALAFIAVGRYGDAEEVLVMAMALKS
                     ENDVLGAELRNDLAEVRRLLSSSSG"
     gene            complement(3814..4734)
                     /gene="argF"
                     /locus_tag="VFV99_08640"
     CDS             complement(3814..4734)
                     /gene="argF"
                     /locus_tag="VFV99_08640"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5059412.1"
                     /translation="MKRGFLTLGDVPVAQWPALFDRAAYLKKNRNQTTLAGKAVALVF
                     EKASTRTRVSFEVGVFELGGHAVVLTSQGSQIARGEPIEDTARVLSGYCHAIVLRTFG
                     QERVAQLAKAAWVPVINGLSDQHHPCQVATDLFTVREKFGRIEGLRYAWIGDGNNMAA
                     SWIEAAGMFGLDLILACPEGFAPDPLLVNAARELQKQNGRGSLTLTKDPKEAARGAHV
                     ISTDVWASMGQEEEASRRKLAFAGYCVDQAIVKSGAPDAIVLHCLPAHRGEEITGDVL
                     DGPSSLAWVQAENRLHVGKAILEHVMTGRE"
     gene            complement(4731..5957)
                     /locus_tag="VFV99_08645"
     CDS             complement(4731..5957)
                     /locus_tag="VFV99_08645"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5059413.1"
                     /translation="MHTQADLLALADQVLLKNNKPQPIVFERGRGCEVWDRAGTRYLD
                     MTAGIAVCGLGHSHSLFTARVTEQLLKLVHISNLYYNDQQILAAKNIVDRSFADRVFF
                     CNSGAEANEGALKLARRYQAVVEGQPQRTKIVSTIGSFHGRTFATVAITGQAKYRENF
                     GPLVEPVEHIAYGDLEAATKALEGRAACAIIVEPIQAEGGIVVAPPGYLAGLRRLCDD
                     TGTILIFDEVQTGIGRTGTWFGHQHDDVTPDVMTLAKGLGGGVPVGAVAMTNKAAAGL
                     SMAPGGAVPHATTFGGNPLACAAANAVIEIIDNEGLLERVSQAGQYLGGKLNELVKEF
                     PGHALDTRGRGLLRGIAVKGAPAAVTTRCREKGMLVSVAGSNVIRFAPPYIVERHQLD
                     EAVGILRSVLAEGVGK"
     gene            complement(5978..7333)
                     /gene="hslU"
                     /locus_tag="VFV99_08650"
     CDS             complement(5978..7333)
                     /gene="hslU"
                     /locus_tag="VFV99_08650"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5059414.1"
                     /translation="MKTEQELTPAKIVAELDRYVIGQGEAKRAVAVALRNRWRRQQVE
                     GELRDEISPKNIIMIGPTGVGKTEIARRLARLARAPFLKVEASKFTEVGYVGRDVDSI
                     VRDLDEVSVKLVRDEEAGKVRPRAKEAAEERLLDLLLPRGGGRAFTDRPGEPSTANDA
                     TREKLRAMLRDGKLDDREVEVELTDDSNPLISVLGGQPGIDESALSGLKDMFGSLGRK
                     TKRQKMKVQQAWRALTEQEVDKLIDHDAATREAVRRAENAGIVFIDEIDKIATKAERH
                     GADVSREGVQRDLLPIVEGSTVTTKYGPVRTDHVLFIAAGAFHMAKPSDLIPEMQGRF
                     PIRVELKSLGADEFVRILTEPTNALTRQYQALLATEGVNIEWTADAVRELAQIAADVN
                     QRTHDIGARRLHTVLERLLDDLLYNAPDIGAQTIPITPQYVRERLGALVADEDLTRYI
                     L"
     gene            complement(7330..7881)
                     /gene="hslV"
                     /locus_tag="VFV99_08655"
     CDS             complement(7330..7881)
                     /gene="hslV"
                     /locus_tag="VFV99_08655"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5059415.1"
                     /translation="MKQPEIHSTTILSVRRGSQVVVAGDGQVTLGNQVVKGGAKKVRR
                     LGEGRAIGGFAGSAADGIALFERLDDKLREQRSPLRRAAVELAKEWRTDRALRRLEAM
                     LVVADGESTFIISGSGDVIEPDDGVCAIGSGGGFALAAARALVRTPEAGSLSPRQIAE
                     TAMAIAADICIYTNTSLTIEELP"
     gene            complement(7963..8814)
                     /locus_tag="VFV99_08660"
     CDS             complement(7963..8814)
                     /locus_tag="VFV99_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059416.1"
                     /translation="MSMPDWLVPMAATLTQERFTGPEWTFELKYDGIRLISFKRGSEV
                     KLYSRNHLEQTVPHVSAAIAKLPHDEVILDGELAWDGSEYHVFDMLWIDGRDLRALTL
                     EQRRAELAKLPLAPPLERALIVDDDEPWERARREGWEGVIAKRRDSVYEHKRSKQWLK
                     MKIEASQELVVGGFTDPQGQRVGLGALLVGFYEGEDFVFAGKVGTGFDTKLLRELREK
                     LDALAIERSPFTRAVGLPKLRVHWVRPDIVVQVGFMEWTGHDKLRHPRLIGVRYDKTA
                     RGVVRDR"
     gene            complement(8827..9786)
                     /locus_tag="VFV99_08665"
     CDS             complement(8827..9786)
                     /locus_tag="VFV99_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5059417.1"
                     /translation="MDWDAALAAFKSYLEVERAYSAKTVEVYLRDVGALRDHLREKRG
                     KDAPLARLSAIDVRSQLAALFGENGAATIGRKLSSVRAFCRFLVKRGVLAGNPAAAIR
                     GPKKGKGLPRALDVDDAFRLVEAPTTTGRTAHRTLSAEEEARHALLRLRDQAMLELIY
                     STGLRVSEACSLDVSDIDRDRYGMPIVLVRRGKGNKSREVPLGGAADRALAAYLPARR
                     ALKAVDQALFVNAGGERLTPRSVQRMVKRWTIAGGVHANATPHGLRHSFATHLLDEGV
                     DLRSIQELLGHASLSSTQIYTKVSLDHLMKVYDDAHPRAKPSK"
     gene            complement(9838..10527)
                     /locus_tag="VFV99_08670"
     CDS             complement(9838..10527)
                     /locus_tag="VFV99_08670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5059418.1"
                     /translation="MQRLIGNLALVTGGSSGIGLACARVLAAEGAGVLSCARRFARKP
                     VTVPGAGEIVETHLDVTDEAQVRAVFAVLPYVDVLVCSHGTGTFAPIANANVADVRAM
                     LDVHITGSFLCAREALRLMHAKHSGHIVLVSSHAAHHGFTECGGYTAAKAGQLGLGRV
                     LAAEARPHNIRVTTLMPGATDTPIWDDRPGFDRSKMMQPGDVAQLLLALVTNPAISVD
                     EVTVTPPAGVL"
     gene            10550..11017
                     /locus_tag="VFV99_08675"
     CDS             10550..11017
                     /locus_tag="VFV99_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059419.1"
                     /translation="MRLAGIVVAIVVGWSVAYAGSKPLTTEQALKPYAGRIVFSPDVP
                     PTQLDEMPAYLKANATKENSYDVLKGPPWQFHLVSVLAKDPGTKPISLVFADKDDKKL
                     TAMHTVEVHSQKKLVITASEATIAAGFAAGKTYVVRLMQGKTVLAKAELHLRE"
     gene            complement(11018..11557)
                     /locus_tag="VFV99_08680"
     CDS             complement(11018..11557)
                     /locus_tag="VFV99_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059420.1"
                     /translation="MTRVIIGDKPTIDVRDPRPGDLGWVVERHGVLYAEEYGWDVRFE
                     ALVARVVADFVEHKHASDRCWIAYVKGKRAGSIFCVRGSDTVAKLRLLLVEPWARGLG
                     IGHHLVREVIEHALANGYRTLSLWTNSVLVSARKIYEGEGFVLAHEEPHDRFGAGLVG
                     QTWELDLTRWSPNPARLTH"
     gene            complement(11554..12930)
                     /gene="trmFO"
                     /locus_tag="VFV99_08685"
     CDS             complement(11554..12930)
                     /gene="trmFO"
                     /locus_tag="VFV99_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5059421.1"
                     /translation="MRPDVTVVGGGMAGSEAAWQLAEAGLCVSLVEMKPCAMSPAHQS
                     PLLGELVCSNSLRSDDPIAPAGLLKHELRRAGSVVIACADQHRVPAGQALAVERFGFA
                     RAITQRLALHPRIRIERRRIDELPDHPVIVATGPLTEGALGDVIRAELGGDRMYFYDA
                     IAPIVAADSIDFDFAFRASRWGRDADAPGRDADGDRAAGGDTGVGDYINCPLNKEQYD
                     AFVAAVNAGRKVLPHDFEEPRYFESCMPIEVMAERGDQTLRFGPMRPIGLRDPRTGHR
                     PWAVVQLRPENRYQTAYNLVGFQTRLAYPEQQRIFQMIPALHRAEFLRFGSIHRNTYI
                     DAPSRLGSHLELRDRPNVRFAGLLTGVEGYIESCAMGMIVAWLLAGELTGRAAPYPPP
                     TTMMGGLYNHVTAPREGNYKYGPTNVNYGLLPPLPDVRKDHRKHRMAERAQADFDAWL
                     GRVAVRAA"
     gene            complement(13109..13447)
                     /locus_tag="VFV99_08690"
     CDS             complement(13109..13447)
                     /locus_tag="VFV99_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059422.1"
                     /translation="MAKAKKAAKAKKGAKKAASKPAKKSAAKKAKPAKKGAVKAKAKA
                     KAKAKKPAAKKPAAKKAPVMTAPAPAPAPMDTAPPSAPDTDLDNMPPPLPEVDEGATD
                     EGWDSSEAAS"
     gene            13657..14211
                     /locus_tag="VFV99_08695"
     CDS             13657..14211
                     /locus_tag="VFV99_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059423.1"
                     /translation="MDWPCPGGTQDCLGFPATPDDEPIGEGPGGTPLPDVDDDLTGPH
                     RPGSPVQASGCPATPWWGGNLRATGFAVFVGGGRAPAIDADASLRGEVDGLLPEELHA
                     PVIHSLDRLYACLCAPTTSVRIDVRIVINPTGRTLSVDSNAPDRACMRDALARIAFPR
                     RDSPTFARFRLEYTVKTTQPVRLP"
     gene            14208..14936
                     /locus_tag="VFV99_08700"
     CDS             14208..14936
                     /locus_tag="VFV99_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059424.1"
                     /translation="MIALSFAAMANFTTWTVCKHKPIEKLTPNLWRVSGTMPNAETQQ
                     RQMVLARMADGRVIVHNAVALDDNEMAELEAWGTPSVIFVPNAYHRQDAGIWKQRYPQ
                     AKVITPAGAKKRVNTAVAVDAVTEEAPKDDTVRLFPIDGLPGESVLEVKSPDGVSLTF
                     CDAILNVPKLGFPMGFFLGPTGQVSTPRVVRWIALKNKKAFAAQLEKLAETPGLNRVM
                     FGHGRPITDDPKGALLAVVKQLVG"
     gene            complement(15266..17878)
                     /gene="topA"
                     /locus_tag="VFV99_08705"
     CDS             complement(15266..17878)
                     /gene="topA"
                     /locus_tag="VFV99_08705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5059425.1"
                     /translation="MTKKKAPPPKKKPVAAKGNGKAKGAPAKAAAKKPEPKKPEPKKP
                     EPKKPEPKVEAKKADKKADRAAAKKPVKGAKPSKATGKTAENGEEEEPKIVRAKKIKP
                     GSALVVVESPAKAKTINKYLGANYIVKASVGHVRDIPKKPRFKGDIGIDLDDGSFEPA
                     YEVIEGKKKVLGDIRRAAASTETVYLASDPDREGEAIAWHVAEEIKDVNHNLKRVLIN
                     EITKKGVTAAIAAPRAIDMDKTNAQQARRILDRIVGYKISPILWNKVQRGLSAGRVQS
                     VAVRLVCEREEEIKAFVPEEYWSVDITCRADQPPPFEARIWKWKGEKAEPKTQADAEA
                     IATELRAGNAVVATVDKKERRKKPQAPFITSRLQQDAARKLRFSAKRTMALAQRLYEG
                     VELGEEGPTGLITYMRTDSTRVSDDALTAVRQYVTDTYGKEYLPDEVNTYGNKDRAQD
                     AHEAIRPTLMEWPPEKVEEALANHPEGVELTKLYRLIWQRFVASQMVPAVYDQTTVDM
                     DRGEAVIRANGQVMKFAGYTKVYEVAESDDAKAEAAEAADKLLPPLEVGDSVTLESVR
                     PEQHFTQPPPRFSEASLVKELEERGIGRPSTYAAIMSTIVDRGYVEKKEARFWPTELG
                     ILVNGLLVESFPEIVSSDFTAKMEADLDHVEEGREDWRKLLGAFYGPFKGELEKAETE
                     MRDIKREEQPTEWSCEKCGKPIVIKFGRNGYFAACSGYPECRTTFEVVKNLDGTWEKV
                     PPQTTDEVCETCGAPMTVKRGRFGSFLACTRYPDCKTTKPISLGVKCPRPGCGGFIAE
                     KRSRRGKPFYGCSNWAKKGCDFVAWDKPIPQPCPVCQAKFVLKKENKSGTTLRCMECD
                     WRSQGSDESEENAA"
     gene            complement(17875..18672)
                     /locus_tag="VFV99_08710"
     CDS             complement(17875..18672)
                     /locus_tag="VFV99_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5059426.1"
                     /translation="MTTALSSLRPGDAGYPQRLRALGIEQVLHVRGQLCEGPAVAIVG
                     ARAATTKAMGRAHTWAKHVVARGVQVVSGGALGIDAAAHRGALAGAGTTTVVLGSGVD
                     VLYPARHAGLFHQILASDGALVSTFPHGMQPRPGTFTQRNTIISALADAVIVVEADVK
                     SGSLSTAAAARKQGRIVAAYPGSPGCAKLLATGAALVETDEDVVELLAGRPRYPAPLV
                     LDDNATRVRDALAQGVRGVDAIVRHTGLSVRAVLRALPQLESLARLQ"
     gene            complement(18806..20089)
                     /locus_tag="VFV99_08715"
     CDS             complement(18806..20089)
                     /locus_tag="VFV99_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5059427.1"
                     /translation="MRKILLALVAPLVAPGVAAAQVPVYPQQQDAPINLDPGAAPKPA
                     TPPPAPKQQPQKPNVVIVGPDGKVQYAPDQQPGAAGAPAGGYYYAPEGSVPFGYDTAD
                     EGGPVSVHAGPTPELHVVRKGDTLWDICFYYFNDPWQWPKVWSYNPQITNPHWIYPGD
                     LVRLLPRGMFAQQPAQEPEQTPQPGEVKPIDNIPPPATRSDVGIKQTAFVEKSDLEKS
                     NVVDGAVDEKDLLTIGDSIYVSYPANNPPKVGQLYSIYKPDNSVKHGGKEQGAYVRVL
                     GTVRIETVKQDKKARGVIVEANQEIERGARVGPLAKTFKNVPPVPPKVDAQGNIVAML
                     KQDQLIGQGEVVFVDLGKSSGIEVGNRMFVVRRGDAKPKQSKDTVGQDDRTFPARSLG
                     QIVIVEVGDQVSIGLVTLSVQEMAVGDIVMMQKSQ"
     gene            complement(20086..20910)
                     /gene="ybgF"
                     /locus_tag="VFV99_08720"
     CDS             complement(20086..20910)
                     /gene="ybgF"
                     /locus_tag="VFV99_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX5059428.1"
                     /translation="MTITRHLFLVALASVGCATPVKALREDNRKLSMQVAELRADRRI
                     QDRKLRDMEHQLAQMKVTSQAMEPMPTLPVEVVAPPTVNDGQRIVAVADDGSEIVYEG
                     DAALGKPAAVEHPRRPAAVPVRSDRLEVTKRPAPMSARTTHVPTRSDGNDAAGEDYRA
                     AVELVKAGKTTDAVTALRTFLKNYPRHEYADNAQYWLGETFYASKDYQHALVEFRATI
                     ETYPRGNKVPDALLKVGYCYSALGQADKARAVLEQVVNLYPKSEPAALAAKRLESP"
     gene            21031..22347
                     /locus_tag="VFV99_08725"
     CDS             21031..22347
                     /locus_tag="VFV99_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059429.1"
                     /translation="MRGVVVVCMACVASASASAQPAPVPSPPTPRDHRDSPEPPPPPP
                     APRDHRDEPPPPIVPQPTTSDTPAPANPPVAVEAGADVSAGVHNGTVTLPAILVHIEY
                     GLDFGIGPTRGAQPTQGYAVVFGLPVNVFLLPWLGVGVRGQVVYSQDPAADGPMTPYR
                     SDDVETDPLAAWSATAGPRFRFWSDPAARLRWVVDVDAGYVHPDPISPAGPLARLSLG
                     RQVGIFNPQGNGANLGVSLAAETGLGPAASYRAFYASAWFHGESGVFAATDPGDDGAW
                     FPSTFSIDMPMLLGKNFGDVPGNLIFGVAAAFGVAVSDLLEPRIRIDTHYIRHSDQDA
                     TKTLGASAGTRLRFDHYGAPAFVDLYAGYEHAYGVEPRLIGNGRFVDVGTGLHIVACK
                     FEYDLGVRFRLGVSDDNSKLADIALVLGGARGNTVTALGKPIGKCD"
     gene            22470..>22984
                     /locus_tag="VFV99_08730"
     CDS             22470..>22984
                     /locus_tag="VFV99_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059430.1"
                     /translation="MRNFALVLVLVACSSSTTTTPSSKLAIDRNNVTDFDATVREGPA
                     WARVVVRYTPTTTFYELSTSYGLLAFAGTEVNFSAGDPGVDQSVRELLADSRATDDGY
                     VILAAGDPEYDLVEGLLDQLIAAGAVQSPTLEQKGTTLYAAAYTTARVLGMQLATHAP
                     GTGGRHGGGAH"
BASE COUNT         3613 a         7785 c         7374 g         4212 t
ORIGIN      
        1 cagcggcggc gcttcggctt cgatctcgat ctgtgccgaa gacctgcgtg gcggcgcgtc
       61 ggcttcgatc tcgatctcgc ccgacgacct gcgcgcgtgg acgttgatgt cgtgcgtcgc
      121 ttcctcgtcg aaccagccgg cgtcgacttc gtcgttggcg agcaccggtt gcgactcgcg
      181 ctgcggcaga tcgcccggca ggatctcgat ctgatactcg ggctcctctt cgattccgaa
      241 cggtgcttcc tcggcaccct cgacgtgcgg ctcgctgctc agcagctcgt agtgaagccg
      301 cacgtagtcg acgtagctgc tcgcccgcgc gttgtccgga tcgatcgcga gcgcttcctc
      361 ccacatcagc aaggcaccat cgaggtcccc tgcgccgtag cggttcagtc ccagctcgat
      421 gagccgctcg atatcagacg gtgggcccac cgcagatcat ccgcgtgtct tggacaccac
      481 tccgcggaac atgttgagcg tacggttgag cgcctcggta gcatccgcca gcgtcgtcag
      541 atccttcgcc gtgatcgctt ggcgcgtacg ctccacgacc gcacgcgcct tcttgaccgc
      601 gtcctgaccg aagtccgagc ccgcgatggc gtcggtgacc tgcgggaaca gcgattcgat
      661 ctcctcgagt gacttctctg cctgatggcg cgccttgtcg aactcctggc cggtgcggac
      721 ctcgaccatg tagtccttgt tcgcgtcgat catcttcttg atctcgtcct cggtcagacc
      781 cgaggtcgcc gtgaccgtga tcgattgctc gaggccggtc tcgagatcct tcgccttcac
      841 gctgacgatg ccgtcggcgc tgatgtggaa ctggacctcg atctcgactt cgccacgcgg
      901 cgcgcgccgc aagccggtga gcacgaactc gccgagtagc tcgttctcct cggcgcgatc
      961 gctctcgcct tggaggacga ggatcttcac cgaggtctga ttgtctttga ccgtggtgaa
     1021 cacgtgcgac tcgctcgttg gcaccgtggt gttctgctcg atcagtttgt ggaagtagcc
     1081 gccgacgatc atgatgccga gcgagtgcgg cgtgacgtcg agcaggagga tgtcgctctt
     1141 ctcgtcggtg agcgccgcac cttggatcgc ggcaccaagc gccacgacct cgtcgggatg
     1201 cacgcccttg cacggctcga ggccgaagaa ctcggcgcac gcctgctgca cgcgcggcat
     1261 gcgcgtcatg ccgccgacga ggatcacgtc ctcgatctgg ttcttctgga tctcggcttc
     1321 ttcgagcgtg cgcgcgcaga tctcgacggt gcgctcgacg agatcgctcg tgagctcttc
     1381 gagcttgtcg cgcgtcagca tcgtctgcag gtgcaacgct tcgttgcgac cggtcgagat
     1441 gatgaacggc aggttgatct cggtttcctt gacgctcgac agctcgcact tcgccttctc
     1501 ggcgacgtcc ttgagacgct gcagcgccat cttgtccttg cggaggtcga tgccgtgctg
     1561 cttctcgaag ccgaatacga gccaatcgat aatgcggcga tcgaaatcct cgccgccgag
     1621 gaacgtgtca cctgcagtcg agatgacctc gaacacgccg ttgccgatct cgaggatcga
     1681 aatatcgaag gtgccaccgc cgaggtcgta gaccgcgacc ttgcgctcga tgttgcggcc
     1741 aaagccgtat gcgagcgccg ccgccgtcgg ctcgttgata atgcggatga cgtcgagacc
     1801 cgcgatgcga cccgcgtcct tcgtggcttg gcgctgaccg tcgttgaagt acgcgggcac
     1861 ggtgatgacg gccttctgaa cttcctcgcc gaggtattcc tcggagatca gcttcatctc
     1921 ctgcaggatg tacgcggaga cttccggcac gctgaacacc tggtcgcgca gcatgatgcg
     1981 gacgtcgtca tgcggacctt cgacgatccg gtacggcatg atctccagcg agctgcgcac
     2041 cgcggcgctg ccccacttac gaccgatcag gcgcttcgcc gcgtagacgg tgttctctgc
     2101 gttggtgatc gcttgacgct ttgcgatatg cccgacgagg cgcttgccat tctcggcaat
     2161 tgccaccatc gatggtgtcg tcttgtaccc acctcggttc ggaatgaccg aaggcgttcc
     2221 gtcctcgaca atcgcaacgc acgagttagt cgtgcctagg tcgatgccga tcaccttctc
     2281 actggccatg gcggattagt ttcccccaga aatcgaggag atcgccagtg caggcgcctc
     2341 ccaagtgagg agagtaaccc agttcgctcc cgtcaaccac tcgacgagga caatagccgg
     2401 cgaacctccg ccaggtcgtt gcgaagctcc gctccgagca catcgttctc gctcttgaga
     2461 gccatcgcca tgacgagcac ttcttcggcg tcgccgtagc gaccgactgc aatgaatgcc
     2521 agcgcgagca ggcgtgccga cgacggatcg tttggcgcct gcgcttgcgc ctttttcgca
     2581 tactcgagcg cggtcttcgg ctgctcggcg gcgagcgcgc acgttgcggc gcgccgcaga
     2641 tagcgggttt gtggatcaat ccgtaccgct gccgcgaaca atttcatcgc gccgagcgcg
     2701 tcgccgccga cctcgagccc acacgcgcgc tggaacagct cgcccgcgta ctcgcccggc
     2761 gtctgcggct gttcggtctg caccatcgga tacgcgccgc tgcgttgatc tttggcaggc
     2821 gacgtcaggc gcgcatacgc acggctcacc gcctcgaaca ccacgctgag ccggtcggcg
     2881 aacgtgccga gctgcttgcc gtagtggcgg tcggggtgga tctccttgga cagcgtgaag
     2941 taggcgcgct tgaggacctt cgcgtccgag ccggtaggaa caccaagtaa tgcccacgga
     3001 tcgcgttcgt cgaccagccg tgccatcacg aggatccgca cgcgctcgtc ggcatcgaga
     3061 tcgcccggct cggccagcgc gctcttctcc tccgcggtcg gctgcggaag catgaggtgg
     3121 agctcggcgg tcgacgcgcg gcgaacggtc ggtggatcga cgctctcggg cgcgggcgga
     3181 cgcatccctg ccgccggatg cgtgccgagc tgggtgcgcg gctgggttgg cgggtcggcg
     3241 ggcggcctgg acggtggcgc cggccgcttc gcgtccatcg gcaagtgcgg cgtcatcgtc
     3301 cgcggcactg tcggccgctc gccggtcccc gggcgcatcg gcgttcgtcc agtcaccggg
     3361 agatgcggcg tgatcgtacg cggcggcagg accgccggca tttcgtgggg cgtcacgcgt
     3421 acggtcggcg cgcggcccat ctgctcggcc acggggctgg gtacgccatg cggtggcgtg
     3481 agcggcgcga ccggtgtcgt cgagttcggc cgcggtcccg ttccgcgcgc gggcacggac
     3541 atcggtgtcg gcgctgcagt ctcgcccgga aggagtagcg cgccgatcga gcgcagcttc
     3601 gtcacgatcg cgatcccgcg atcgacaggc aggccggtgg cgagcaggat ctcgcgaatc
     3661 gactgcgtgc cgtcgatgcg cgacagcacg aagtattcgt ccggaccgac ctgtgccttg
     3721 agcgggtcga acccagggct gacccgcagc ttcatcgacg acgatccgag tcccacgatc
     3781 tcggacggga cgctgctcac gatcacaatc ctatcattcg cgaccggtca tgacgtgctc
     3841 gagaattgcc ttgcccacgt gcaagcgatt ctctgcctgg acccaggcca gcgagctcgg
     3901 accgtcgagc acatctccgg taatttcttc gccccggtgc gcaggtaggc agtgaaggac
     3961 gatcgcatcc ggcgccccgc tcttcacgat cgcctggtcg acacagtaac cggcgaaggc
     4021 cagcttacgt cttgacgcct cttcctcttg ccccatcgac gcccacacat ccgtcgagat
     4081 gacatgcgct ccgcgcgccg cctccttcgg atcctttgtc agcgtaagtg aaccgcgtcc
     4141 gttctgcttc tggagctcac gcgctgcgtt gacgagcagc ggatcggggg caaaaccttc
     4201 cgggcacgcg aggatcagat cgagcccgaa catgcctgcc gcctcgatcc acgacgccgc
     4261 catgttgttg ccatcgccga tccaggcgta gcgcaggccc tcgatgcggc cgaacttctc
     4321 gcgcacggtg aacagatcgg tcgcgacctg acacgggtga tgctgatcgg agagtccgtt
     4381 gatcaccgga acccacgcag ccttcgcgag ctgcgcgacg cgttcctgac caaacgtgcg
     4441 cagcacgatc gcgtggcaat agcccgatag cacgcgcgcg gtgtcttcga tcggttcgcc
     4501 gcgcgcgatc tggctgcctt gcgaggtgag gaccacggcg tgaccaccga gctcgaacac
     4561 gccgacctcg aacgagacgc gcgtgcgcgt cgacgctttc tcgaacacga gcgcgacggc
     4621 cttgccggcg agcgtggtct ggttgcggtt cttcttgagg tacgccgcgc gatcgaacag
     4681 agccggccac tgcgcgaccg gaacatcacc gagcgtcaaa aatccgcgct tcacttgcca
     4741 actccttctg cgagcacgct ccgcaggatg ccaaccgctt cgtcgagctg atgccgctcg
     4801 acgatgtacg gcggcgcgaa gcgaatgacg ttggaaccgg cgaccgacac gagcatgccc
     4861 ttctcgcggc agcgcgtggt gacagcggcc ggcgcaccct tgacggcgat gccacgcaag
     4921 agaccacggc cgcgtgtgtc gagtgcgtga ccggggaact ctttcacgag ctcgttgagc
     4981 tttccgccca ggtactggcc cgcttgcgac acgcgctcga gtaagccctc gttgtcgatg
     5041 atctcgatga ccgcgtttgc cgccgcgcac gcgagcggat tgccgccgaa cgtcgtcgcg
     5101 tgcgggacgg caccgccggg cgccatcgag aggcccgccg ccgccttgtt ggtcatcgcg
     5161 accgcgccga cggggacgcc gccgccgagg cccttcgcga gcgtcatcac gtcgggcgtg
     5221 acgtcgtcgt gctgatgacc gaaccacgta ccggtgcggc cgatgccggt ctgcacctcg
     5281 tcgaagatca ggatcgtgcc ggtgtcgtcg cataggcggc gcaggccggc gagatagccg
     5341 ggaggtgcga cgacgatgcc gccttcggcc tggatcggct cgacgatgat cgcgcacgcc
     5401 gcacgtccct cgagcgcctt cgtggcggct tcgagatcgc catacgcgat gtgctcgacg
     5461 ggctcgacga gcgggccgaa gttctcgcgg tacttcgcct gaccggtgat cgcgaccgtc
     5521 gcgaacgtgc ggccgtggaa gctgccgatc gtcgacacga tcttcgtgcg ctgcggctgg
     5581 ccctcgacga cggcttgata gcgacgcgcg agcttgagcg caccctcgtt cgcttccgcg
     5641 cccgagttgc agaagaacac gcgatcggcg aacgagcgat cgacgatgtt cttcgcggcg
     5701 aggatctgct gatcgttata gtagaggttc gagatgtgaa cgagcttgag cagctgctcg
     5761 gtcacgcgcg cggtgaacag cgagtggctg tggccgagcc cgcacacggc gatgcccgcg
     5821 gtcatgtcga gatagcgggt tccggcgcga tcccagacct cgcagccacg cccacgctcg
     5881 aacacgatcg gctgcggctt gttgttcttg agaagaacct gatcggcgag tgctaggagg
     5941 tcagcctgcg tatgcacggc cgaggtgtac caccaactta gaggatgtag cgcgtcagat
     6001 cttcgtccgc gacaagggcg ccgagccgct cgcgcacgta ctgaggggta atcggaattg
     6061 tctgcgcgcc gatatcgggc gcgttataga gcagatcgtc gagcagccgc tcgagcaccg
     6121 tgtggagtcg gcgcgcgccg atgtcgtgcg tgcgctggtt gacgtcggcg gcgatctgcg
     6181 cgagctcgcg aaccgcgtca gccgtccact cgatgttgac accttcggtc gcgagcagcg
     6241 cctggtactg gcgggtgagc gcgttcgtcg gctcggtcaa gatgcgcacg aactcgtccg
     6301 cgccgagcga cttgagctcg acacggatcg gaaagcgccc ctgcatctca gggatgagat
     6361 cgctcggctt cgccatgtgg aacgcgccgg ccgcgatgaa cagcacgtga tcggtgcgca
     6421 ccgggccgta cttcgtcgtg accgtcgagc cttcgacgat cggcaacagg tcgcgctgca
     6481 cgccttcgcg cgacacgtcg gcaccgtggc gctcggcctt cgtcgcgatc ttgtcgatct
     6541 cgtcgatgaa cacgatgccg gcattctcgg cgcgccgcac cgcttcgcgc gttgcagcgt
     6601 cgtgatcgat cagcttgtcg acctcttgct cggtgagcgc gcgccacgcc tgctgcacct
     6661 tcatcttctg ccgcttggtc ttgcgaccga gcgaaccgaa catgtccttg aggcccgaca
     6721 gtgctgactc gtcgatgccg ggctgaccgc cgagcaccga gatcagcggg ttcgagtcgt
     6781 cggtgagctc gacttcgacc tcgcgatcgt cgagcttgcc gtcgcgcaac atcgcacgca
     6841 gcttctcgcg cgtcgcatcg ttcgccgtgc tcggctcacc gggtcgatcc gtgaatgcac
     6901 gaccgccgcc gcgcggcagc aagagatcga gcagccgctc ctcggcggcc tccttcgcac
     6961 gcggccgcac cttgccggct tcctcgtcgc gcacgagctt gaccgacacc tcgtcgaggt
     7021 cgcgcacgat cgagtcgacg tcgcggccga cgtagccgac ctcggtgaac ttcgacgcct
     7081 cgaccttgag gaacggcgca cgcgcgaggc gcgcgagtcg acgcgcgatc tcggtcttac
     7141 cgacgccggt cggtccgatc atgatgatgt tcttcggcga gatttcgtcg cgcagctcgc
     7201 cttcgacctg ctggcggcgc cagcgattgc gcagcgcgac cgcgaccgca cgtttcgcct
     7261 cgccctggcc gatcacgtac cgatcaagct cggcgacgat cttggcaggc gtgagctcct
     7321 gctcggtctt cacggcagct cctcgatcgt caaactcgtg ttcgtgtaga tgcagatgtc
     7381 ggcggcaatt gccatcgccg tctctgcgat ctggcgcggc gagagcgagc cagcttccgg
     7441 tgtccggacc agcgcgcgtg ccgctgcgag tgcaaagccg ccacccgagc cgatcgcgca
     7501 cacgccgtcg tcgggctcga tcacgtcgcc ggagccggag atgatgaacg tactctcgcc
     7561 atcggcgacg acgagcatcg cttcgagccg gcgcagcgcg cgatcggtgc gccactcctt
     7621 tgcgagctcg accgcggcgc gacggagcgg gctgcgttgt tcacggagct tgtcgtcgag
     7681 acgctcgaac agtgcgatgc cgtcggcggc cgagccggcg aatccgccga tcgccctgcc
     7741 ctcgccgagc cggcgcacct tcttcgcgcc gcctttgacg acctgattac cgagcgtgac
     7801 ctgtccgtcg cctgccacca ccacctgcga acctcgccga acggacagga tcgtcgtcga
     7861 gtggatctcc ggctgcttca cgcgccgttc gtagcacgcg cgcggcgcca gcggcagggc
     7921 tatcgctcgc gcgtcatctg tcgcgcacca ccctctcgcg cactatctgt cgcgcaccac
     7981 ccctcgcgcg gtcttgtcgt agcggacgcc gatcagccgg ggatggcgca gcttgtcgtg
     8041 ccccgtccat tccatgaagc cgacctgcac gacgatgtca ggtctcaccc aatggacgcg
     8101 cagcttcggc aacccgaccg cgcgggtgaa cggcgagcgc tcgatcgcga gcgcgtccag
     8161 cttctcgcgc agctcgcgca acagcttcgt atcgaacccg gtgccgacct tgccggcgaa
     8221 cacgaagtcc tcgccttcgt agaagcccac gagcagcgcg ccgaggccga cccgctggcc
     8281 ctgcggatcg gtgaagccac cgacgacgag ctcctgcgac gcttcgatct tcatcttgag
     8341 ccactgcttc gatcgcttgt gctcgtagac cgaatcgcgt cgcttcgcga tcacaccttc
     8401 ccagccttcg cgccgtgcgc gctcccatgg ttcgtcgtcg tccacgatca acgcgcgctc
     8461 gagcggtggc gcgagcggca acttcgcgag ctctgcgcgg cgctgttcga gtgtcagcgc
     8521 acgcaggtcg cggccgtcga tccacagcat gtcgaacacg tgatactcgc tcccgtccca
     8581 tgcaagctcg ccatcgagga tgacctcgtc gtgcggcagc ttcgcgatcg cggcactgac
     8641 gtgcggcacg gtctgctcga ggtggttgcg cgaatacagc ttcacctcgc tgccgcgctt
     8701 gaacgagatc agccggatgc cgtcgtactt gagctcgaac gtccactccg gtccggtgaa
     8761 ccgctcttgc gtcagcgtcg cggccatcgg cacgagccag tcgggcatgc tcatggcccg
     8821 ttcactctac ttcgatggct tcgcccgggg gtgcgcgtcg tcgtagacct tcatcaagtg
     8881 gtcgagcgac accttcgtgt agatctgcgt cgacgatagg ctcgcgtggc cgagcagctc
     8941 ctggatcgag cgcaggtcga cgccctcgtc gagcaggtga gtcgcgaacg agtggcgcag
     9001 cccgtgtggc gtcgcgttcg cgtgcacgcc gccggcgatc gtccaccgct tcaccatgcg
     9061 ctgcaccgag cgcggtgtca gccgctcgcc accggcgttg acgaacagcg cctggtccac
     9121 ggccttgagc gcccgccgag ccggcagata cgcggcgagc gcgcggtcgg cagcgccgcc
     9181 gagcggcact tcgcgcgact tgttgccctt gccgcgacgc acgagcacga tcggcatgcc
     9241 gtagcgatcg cgatcgatgt cactcacatc gaggctgcac gcctcggaga cgcgcagccc
     9301 ggtcgagtag atgagctcga gcatcgcctg gtcgcgcaac cgcagtagcg cgtggcgcgc
     9361 ctcttcctcc gccgacagcg tgcgatgcgc ggtacggccg gtcgtggtcg gcgcctcgac
     9421 gaggcgaaac gcgtcgtcga catcgagtgc gcgcggcagg cctttgcctt tcttcgggcc
     9481 acgaatcgcc gcggctggat tgccggcgag cacgccgcgc ttgacgagga agcgacagaa
     9541 cgcgcgcacg ctcgacagct tgcggccgat cgtggcggcg ccgttttcgc cgaacagcgc
     9601 ggcgagctga ctgcgcacgt cgatcgcgga gagccgcgcg agcggagcgt ccttgccgcg
     9661 cttctcgcgg agatgatcac gcagcgcgcc gacgtcgcgc aggtagacct cgacggtctt
     9721 cgctgaatac gcgcgctcga cctcgaggta gctcttgaag gcggcgagcg ctgcgtccca
     9781 gtccacggtg agaggatcgc gcgaagcgcg ccgcgatgcg acttttcgag cactcggcta
     9841 gagcacgccg gcgggcggcg tcacggtgac ctcgtcgacg gagatcgccg ggttcgtcac
     9901 gagcgcgagc agaagctgcg ccacatcgcc gggctgcatc atcttgcttc gatcgaagcc
     9961 gggccggtcg tcccagatcg gcgtgtcggt cgcgccgggc atcagcgtgg tgacgcgaat
    10021 gttgtgcggc cgcgcttcgg cggcaagcac gcggccaaga ccgagctggc cggccttcgc
    10081 ggccgtgtag ccaccgcact cggtgaagcc gtgatgcgcg gcgtgcgagc tgacgagcac
    10141 gatgtggccg ctgtgcttcg catgcatgag acgcagcgct tcgcgcgcgc acagaaacga
    10201 gcctgtgatg tgcacatcga gcatcgcgcg tacgtcggcg acgttcgcgt tcgcgatcgg
    10261 cgcaaacgtg ccggtgccgt gcgagcacac gaggacatcg acgtagggca gcacggcaaa
    10321 caccgctcgc acttgggctt cgtcggtcac atcgaggtgt gtctcgacga tttcgccggc
    10381 cccgggcact gtcacgggct tgcgtgcaaa cctgcgtgca cacgacagca cgccggcacc
    10441 ttcagccgcg agcacacgtg cgcacgcgag cccaatgccg ctcgagccgc cggtcacgag
    10501 cgctaggttt ccgatcagtc gttgcactca cggcagtata ctcgcgtgca tgaggctcgc
    10561 cgggatcgtg gtcgcgatcg tagtcgggtg gtcggtggca tacgccggca gcaaaccgct
    10621 cacgaccgag caggcgctga aaccgtatgc gggcaggatc gtattctctc cggatgtgcc
    10681 gccgacgcag ctcgacgaga tgccggcgta tctgaaggcg aacgcgacga aggagaacag
    10741 ctacgacgtg ctcaaaggcc cgccgtggca gttccatctc gtatccgtgc tcgcgaagga
    10801 tcccggcacc aagccgatct cactcgtgtt cgccgacaag gacgacaaga agctgacggc
    10861 gatgcacacg gtcgaggtcc actcgcagaa gaagctcgtc attacggcga gcgaagcgac
    10921 gatcgcggcg ggctttgcgg ccggcaagac ctacgtcgtg cggctgatgc agggcaagac
    10981 ggtgcttgcc aaggccgagc tgcacctgcg cgaatagtta gtgcgtgagc cgtgcggggt
    11041 tcggtgacca gcgcgtcaga tcgagctccc aggtctgccc gacgagacca gctccgaagc
    11101 ggtcgtgcgg ttcttcatgc gcgagtacga agccctcacc ttcgtagatt ttccgcgcgc
    11161 tgaccagcac gctgttcgtc cacaggctga gcgtgcgata gccgttcgca agcgcgtgtt
    11221 cgatcacctc ccgcaccagg tgatgtccga tgccgaggcc acgagcccac ggctcgacga
    11281 gcaacaggcg cagcttcgcg acggtatcgc tgcctcggac acagaagatc gaacctgcgc
    11341 gcttgccctt cacgtacgcg atccagcagc gatcgcttgc gtgcttgtgc tcgacgaaat
    11401 cggcgacgac gcgcgcgacc agtgcctcga agcgaacatc ccagccgtac tcctcggcgt
    11461 agagcacgcc gtgccgctcg acgacccagc cgagatcgcc tgggcgcgga tcgcgaacgt
    11521 cgatcgtcgg cttgtcacca atgatgacgc gcgtcatgcc gcacgcaccg caacgcggcc
    11581 gagccacgca tcgaaatccg cctgcgcacg ctcggccatg cggtgcttgc gatggtcctt
    11641 gcgcacgtcg ggcagcggcg gaagcagacc gtagttcacg ttcgtcgggc cgtacttgta
    11701 gttgccttcg cgcggcgcgg tgacgtgatt gtagagaccg cccatcatcg tcgtcggagg
    11761 tggatacggt gcggcgcggc cggtcagctc gccggcgagc agccacgcga cgatcatccc
    11821 catcgcgcac gactcgatgt agccctcgac gcccgtgagc agccccgcga accgcacgtt
    11881 cggccgatcg cgcagctcga gatgcgagcc gagccgactc ggcgcatcga tgtacgtgtt
    11941 gcgatgaatc gagccgaagc gcagaaactc ggcgcggtgc agcgcgggaa tcatctggaa
    12001 gatccgctgc tgctcgggat acgcgaggcg cgtttgaaag ccgaccaggt tgtacgcggt
    12061 ctgatagcga ttctccggcc gcagctgcac gaccgcccac ggccgatgcc cggtacgcgg
    12121 atcgcgtagc ccgattggac gcatcggtcc gaaccgcagc gtctgatcgc cgcgctcggc
    12181 catcacctcg atcggcatgc agctctcgaa gtaacgcggc tcctcgaagt cgtgcggcag
    12241 caccttgcga cccgcgttga ccgcagcaac gaacgcgtcg tactgctcct tgttgagcgg
    12301 acaattgatg tagtcaccga cgcccgtgtc accaccggcc gcgcgatcgc catcggcatc
    12361 gcgacccggt gcatcggcat cgcgtcccca gcgcgaggcg cgaaacgcga agtcgaagtc
    12421 gatcgaatcg gcagcgacga tcggcgcgat cgcgtcgtag aagtacatcc ggtcgccgcc
    12481 gagctcggcg cgaatcacgt ccccgagcgc gccttcggtc agcggcccgg tcgcgacgat
    12541 gacgggatga tccggcagct cgtcgatccg ccggcgctcg atgcgaatgc gcggatgcag
    12601 tgcgagccgc tgcgtgatcg cacgcgcgaa cccgaaccgc tcgaccgcga gcgcctgccc
    12661 tgccggcacg cgatgctgat cggcgcacgc gatcacgacc gagcccgcgc gccgtagctc
    12721 gtgcttgagt aaccccgcgg gcgcgatcgg atcgtcgctg cgcagcgagt tgctgcacac
    12781 gagctcgccg agcagcgggc tctgatgtgc cggcgacatc gcgcacggct tcatctcgac
    12841 gagcgagacg cacaagcccg cttccgcgag ctgccacgcc gcttcgctgc cggccatgcc
    12901 gccgccgacg acggtgacat caggccgcat cgcgcctccg agtagggtca ggcacaactt
    12961 gtactacttg ctgttcgaac aagtagtaga agttgtgtct gaccccgttc caaagcgacg
    13021 tcatcgcgaa tcccccgacc gcgtgacgtg caccgtgaac gttgaccatt gcgtacccag
    13081 aatcgactgc gcttcgcagc gcgcgtgatc agctcgcggc ttcggagctg tcccaaccct
    13141 cgtcggtcgc gccttcgtcg acctcgggga gcggcggcgg catgttgtcg agatccgtat
    13201 ccggagccga cggaggagcg gtgtccatcg gcgcgggtgc gggcgccggc gcagtcatca
    13261 ctggcgcctt cttcgctgcc ggcttcttcg cggcaggctt cttcgccttc gctttcgctt
    13321 tcgccttcgc cttgaccgcg cccttcttcg cgggcttcgc cttcttcgcc gcgcttttct
    13381 tcgcgggttt ggatgcagcc ttcttcgcac ccttcttcgc ttttgctgcc tttttcgcct
    13441 tggccatgcg ccgaacgcta catgaagtcg tcctcgtcgg ggaacaactt cacgaggtcg
    13501 cagcgtcggt ttgcatctgc aacgcgactt gttcggttct gtcttttctt tgtgcgcgct
    13561 ctgtgcatcg ccgcgttggt ggagatctcc tgccgatccc cggcccatcc gagaaccgga
    13621 cagaccgctg caacacagcc ggccgcggac gaggggatgg actggccctg tcccggcgga
    13681 acgcaggact gcctgggatt tccggccact cccgacgacg agcccatcgg cgagggccct
    13741 ggcggcacgc ccctgcccga tgtcgacgac gacctcaccg gcccgcaccg ccccggatcg
    13801 ccggtgcagg cgtccggctg cccggccacc ccgtggtggg gcgggaacct gcgtgcgacc
    13861 gggtttgcgg tgttcgtcgg cggtggccga gcgccggcca tcgacgccga cgcgtcgttg
    13921 cgcggtgagg tcgacggcct cctccccgaa gagctccacg cccccgtgat ccacagcctg
    13981 gaccggctct atgcgtgtct ttgcgcccct acgacgtctg tacggatcga cgttcgcatc
    14041 gtgatcaacc ccaccggtcg cacgctctcg gtcgacagca acgctcccga tcgtgcctgc
    14101 atgcgcgacg cgctcgcgcg gatcgcattt ccacgtcgtg attcaccgac gttcgcgcgc
    14161 tttcgcctcg agtacacggt gaagacgaca cagccggttc gcctgccgtg atcgcgctat
    14221 cgttcgccgc gatggcgaac ttcacgacgt ggacggtctg caagcacaaa ccgatcgaaa
    14281 agctcacgcc gaacctgtgg cgcgtgagcg gcacgatgcc gaatgcggaa acgcagcagc
    14341 gccagatggt gctcgcacgg atggccgatg gccgcgtcat cgtccacaac gcggtcgcgc
    14401 tcgacgataa cgagatggcc gagctcgaag cgtggggcac gccgtcggtg atcttcgtgc
    14461 cgaacgcgta ccaccgccag gacgccggca tctggaagca gcgctacccg caggcaaagg
    14521 tgatcacgcc cgctggcgcg aagaagcgcg tgaacaccgc agtcgcggtc gacgcggtca
    14581 cggaagaagc gcctaaggac gacacggtgc gcttgttccc gatcgacggc ctacccggcg
    14641 agagcgtgct cgaggtgaag tctccggacg gcgtgtcgct cacgttctgc gatgcgatct
    14701 tgaacgtgcc caagctcggc ttcccgatgg gcttctttct cggcccgacg ggtcaggtct
    14761 cgacaccgcg cgtcgtgcgg tggatcgcgc tcaagaacaa gaaggcgttc gcggcgcagc
    14821 tcgagaagct cgccgagacg cccggcctca atcgcgtgat gttcggccac ggcaggccga
    14881 tcacagatga tccgaagggc gcgctactgg ccgtcgtgaa acagctcgtc ggctgatcca
    14941 agcccagcga tccagacgtg cacgtgcacg tgacacgtgc tcgtgctcgt acacgatctc
    15001 tgccggcgaa ctagcgtcgt gatctccgga acgtagtgcc ggcaggcgcg tggttgcgct
    15061 ggccccgagg ctcgaacgcg ttgctgcgcg cgttcagggc gatgctttgt gctccgctcg
    15121 cctgcgggtc ccatggacca cacgatccag gcggccagat cgacaattcg cggtgtccgc
    15181 agggacgtcg ttccaggtgc agaccggacc acctgcctcc gtgcacgtgc acgtgcacgt
    15241 gcacgtgaac gtcccgggct ctgcgctagg ccgcgttctc ttcggactcg tccgagccct
    15301 ggctgcgcca gtcgcattcc atgcagcgca gtgtcgtgcc gctcttgttc tctttcttga
    15361 gcacgaactt cgcctggcac accgggcacg gctgcggaat cggcttgtcc cacgcgacga
    15421 agtcgcaacc cttcttcgcc cagttcgagc agccgtagaa cggcttgccg cgccggctgc
    15481 gcttctccgc gatgaagcca ccgcagccgg gccgcgggca cttcacgccg agggagatcg
    15541 gcttggtcgt cttgcagtcg gggtagcgcg tgcacgccag gaagctgccg aagcggccgc
    15601 gcttgacggt catcggtgca ccgcacgtct cgcagacttc gtccgtcgtc tgcggcggga
    15661 ccttctccca cgtgccatcg aggttcttga cgacctcgaa tgtggtgcgg cattccgggt
    15721 agcccgagca cgcggcgaag tagccgttgc gaccgaactt gatcacgatc ggcttgccgc
    15781 acttctcgca cgaccactcg gtcggttgct cctcgcgctt gatgtcgcgc atctcggtct
    15841 cggccttctc gagctcgcct ttgaacgggc cgtagaacgc accgagcagc ttgcgccaat
    15901 cctcgcgacc ttcctcgacg tggtcgaggt ccgcttccat ctttgcggtg aagtcgctcg
    15961 acacgatctc cgggaagctc tcgacgagca gaccgttgac caggatgccg agctcggtcg
    16021 gccagaagcg cgcttccttc ttctcgacgt atccgcggtc gacgatcgtc gacatgatcg
    16081 cggcgtacgt cgatgggcgg ccgatgccgc gctcctcgag ctccttgacg agcgacgcct
    16141 cggagaaccg cggcggcggc tgcgtgaagt gctgctcggg gcgaacgctc tcgagcgtga
    16201 cgctatcgcc gacttcgagc ggcggtagca gcttgtcagc agcctcggcc gcctcggcct
    16261 tcgcgtcgtc gctctcggcg acttcgtaca ccttcgtata gccggcgaac ttcatcacct
    16321 ggccgttcgc gcgaatcacg gcctcgccgc gatccatgtc gaccgtggtc tggtcgtaca
    16381 cggctggcac catctgcgac gcgacgaagc gctgccagat cagtcggtac agcttcgtga
    16441 gctcgacgcc ttcgggatgg ttcgcgagcg cctcctcgac cttctccggc ggccactcca
    16501 tcagcgtcgg acggatcgct tcgtgcgcgt cctgcgcgcg atccttgttg ccgtacgtgt
    16561 tcacctcgtc ggggaggtac tccttgccgt acgtgtcggt gacgtattgg cggacggccg
    16621 tgagcgcatc gtcggagaca cgcgtcgagt cggtacgcat ataggtgatg agacccgtgg
    16681 gcccttcttc accgagctcg acaccttcat agaggcgctg cgcgagcgcc atcgtgcgct
    16741 tcgcggagaa ccgcagcttg cgcgcggcgt cctgctgcag cctcgacgtg atgaacggcg
    16801 cctgcggttt cttgcggcgc tccttcttgt cgacggttgc gacgacggca ttgccggcgc
    16861 gcagctcggt cgcgatcgct tcggcgtcgg cctgcgtctt cggctcggct ttctcgccct
    16921 tccacttcca gatgcgcgcc tcgaacggcg gcggctgatc cgcacgacac gtgatgtcga
    16981 ccgaccagta ctcctcgggc acgaacgcct tgatctcttc ttcgcgctcg cacacgaggc
    17041 ggacggcgac cgactgcacg cggccagccg acaacccgcg ctggaccttg ttccagagaa
    17101 tcgggctgat cttgtagccg acgattcgat ccaggatgcg acgcgcctgc tgggcgttcg
    17161 tcttgtccat gtcgatcgcg cgcggcgcgg cgatcgcggc ggtgacgccc ttcttcgtga
    17221 tctcgttgat gaggacgcgc ttgaggttgt ggttgacgtc cttgatctct tctgcgacgt
    17281 gccacgcgat cgcttcacct tcgcgatcgg ggtcggatgc gagatagacg gtctcggtcg
    17341 acgcagcggc tctgcggatg tcgccgagga ccttcttctt gccttcgatc acttcgtagg
    17401 ccggctcgaa cgagccgtca tcgagatcga tgccgatgtc gcccttgaag cgtggcttct
    17461 tggggatgtc gcgcacgtga ccgaccgacg ccttcacgat gtagttcgcg cccagatact
    17521 tgttgatcgt cttcgccttt gcaggcgact cgaccacgac gagagcggag cccggcttga
    17581 tcttcttcgc acggacgatc ttcggctctt cttcttcgcc gttctcggcg gtcttgcccg
    17641 tcgccttgct cggcttcgcg cctttgaccg gcttcttggc tgccgccctg tctgccttct
    17701 tgtcagcctt cttggcttcg accttcggct cgggcttctt tggctcgggc ttctttggct
    17761 cgggcttctt cggctcgggc ttcttggctg cggccttcgc gggcgcaccc ttcgccttgc
    17821 cgttcccctt cgcagcaaca ggcttcttct tcggtggcgg cgctttcttc ttggtcactg
    17881 caacctcgct aacgactcga gttggggcaa agcacgtagc acagcgcgaa cagacaagcc
    17941 ggtgtggcgc acgatcgcgt cgacaccgcg cacaccctga gcgagcgcat cgcgcacgcg
    18001 ggtggcattg tcatcgagca cgagcggcgc gggataccgc gggcgcccgg cgagcaattc
    18061 gaccacatcc tcgtcggtct cgacgagtgc tgcaccggtc gcgagcagct tcgcgcagcc
    18121 ggggctgccg ggatacgcgg caacgatgcg gccctgcttg cgcgcggcgg ccgccgtcga
    18181 cagcgagccc gacttcacgt cggcctcgac gacgatcacg gcatcggcga gcgcggagat
    18241 gatcgtgttg cgctgcgtga acgtgcccgg acgcggctgc atgccgtgcg ggaacgtgct
    18301 gacgagcgcg ccatcgctcg cgagaatctg atggaacagg cccgcgtgcc gcgcgggata
    18361 gaggacgtcg acgccactac cgagcactac tgtcgtggtc cccgcacccg cgagtgcgcc
    18421 gcgatgagcc gcggcatcga tgccgagcgc accacccgaa accacctgca cgccgcgcgc
    18481 gacgacatgc ttcgcccacg tatgggcgcg gcccattgct ttcgtagtcg ccgcgcgagc
    18541 gccgacgatc gccactgcgg ggccttcgca gagctggcca cgaacgtgga gcacctgctc
    18601 gattccgagc gcacgaaggc gctgcggata ccccgcatct cccggcctca ggctactcag
    18661 cgcagtggtc acaaggggaa tgcaattagc tacccggcct cgccacgtca agtgcgagag
    18721 atccagcaca agcgatttca gccgcttgca cgaacgccct acatctatat acgcgccaaa
    18781 cgcgctgcgc cgatcaggta gggacctact gcgacttctg catcatcacg atgtcgccga
    18841 ccgccatttc ctgcaccgat aacgtgacca aaccgatcga gacctggtct ccgacttcga
    18901 cgatcacgat ttgtccgagc gagcgcgcgg ggaacgtccg gtcgtcctga ccgacggtgt
    18961 ccttggactg cttcggcttg gcgtcgccgc gacgcacgac gaacatccgg ttaccgacct
    19021 cgatgcccga gctcttgccg aggtcaacga acacgacctc gccttggccg atcagctggt
    19081 cctgcttgag catcgcgacg atgttgccct gcgcgtcgac cttcggcggc accggcggga
    19141 cattcttgaa tgtcttggcc agcgggccga cgcgcgcgcc gcgctcgatc tcctggttcg
    19201 cctcgacgat cacgccgcgc gccttcttgt cttgcttgac ggtctcgatg cgcacggtgc
    19261 cgagcacgcg cacgtatgcg ccctgctctt tgccgccgtg cttcaccgag ttgtcgggct
    19321 tgtagatcga gtagagctgg ccaaccttcg gtgggttgtt cgccggatac gacacgtaga
    19381 tcgaatcgcc gatcgtcagc agatcttttt cgtcgacggc gccgtcgacg acgttcgact
    19441 tctcgagatc cgatttctcg acgaacgcgg tctgcttgat gccgacatcg ctgcgcgtcg
    19501 ccggcggcgg aatgttgtcg atcggcttca cctcaccggg ctgcggcgtc tgctccggct
    19561 cttgggcggg ctgctgcgcg aacatgccgc gcggcaacaa ccgcacgagg tcaccgggat
    19621 agatccagtg cgggttcgtg atctgcgggt tgtacgacca caccttcggc cactgccacg
    19681 gatcgttgaa gtagtagaag cagatatccc agagcgtgtc gcccttgcgc acgacatgga
    19741 gctcgggcgt cggacccgcg tgcaccgaaa ccgggccacc ctcgtcggcg gtgtcgtagc
    19801 caaacggtac ggagccctcc ggtgcgtagt agtagccacc ggcgggcgct cctgccgcgc
    19861 ccggttgttg atcgggcgcg tattgcacct tgccgtcggg accaacgatc acgacgttcg
    19921 gcttctgtgg ttgctgcttc ggtgccggtg gtggcgtcgc gggcttggga gccgcaccag
    19981 gatcgaggtt gatcggtgcg tcttgttgct gcggatacac cggcacctgc gccgccgcca
    20041 cgcccggcgc gacgagcggc gcgaccagcg cgagcagaat ttttctcacg gcgactccaa
    20101 tcgcttcgct gccagcgcag cgggctcgct cttcggatac aggttcacga cttgctctag
    20161 caccgcgcgc gctttgtcgg cctggccgag cgccgagtag cagtatccca ctttgagcaa
    20221 tgcgtcgggc accttgtttc cccgcgggta ggtctcgatc gtagcgcgga actcgacgag
    20281 tgcatgctgg tagtccttgc tcgcgtagaa cgtttcgccg agccagtact gcgcgttatc
    20341 cgcgtactcg tggcgcggat agttcttcaa gaacgtgcgc aacgcggtca ccgcgtcggt
    20401 ggtcttgcca gccttcacga gctcgacggc ggcgcgatag tcctcacccg cagcatcgtt
    20461 accgtcgctg cgcgtcggca cgtgcgtcgt gcgcgccgac atcggtgctg gccgcttggt
    20521 gacttcgaga cgatcgctac ggaccggcac tgccgcaggc cgacgcggat gttcgacggc
    20581 cgctggtttg ccgagggcag catcgccttc gtatacgatc tcgctgccgt catcggcgac
    20641 cgcgacgatg cgctggccgt cgttcaccgt cggtggcgcg acgacttcga ccggcaacgt
    20701 cggcatcggt tccatcgcct gcgatgtcac cttcatctgc gcgagctggt gctccatgtc
    20761 gcgcagcttg cgatcttgga tacgtcgatc tgcgcgcagc tccgccacct gcatgctgag
    20821 cttgcggttg tcttcgcgca gcgctttgac cggcgtagcg caaccgacgc tcgccagcgc
    20881 gaccaggaat agatggcgcg tgatcgtcac aactgagaaa tcgtaggccg gccacgtgaa
    20941 ccgagcaaaa tttcacgcgg acacggatcg aagtacttcg ataagcatca cgtcacagca
    21001 gatggcagcg cgcgttgata agctcgatgc gtgcggggtg tcgtcgtcgt ctgcatggca
    21061 tgcgtggcga gcgcgagcgc gagcgcacag cctgcgcctg tgccgtcgcc accgacgcct
    21121 cgcgaccatc gcgattcgcc ggagccgcca ccgccaccgc ccgctcctcg cgatcaccgc
    21181 gacgaaccgc cgccgccgat cgtgccgcaa ccgaccacga gcgacacgcc ggctcctgcg
    21241 aatccacccg tggccgtcga ggccggagcc gacgtgagcg caggcgtgca caacggcacc
    21301 gtaacgctgc ctgcgatctt ggtgcacatc gagtacggcc tcgacttcgg catcggtccg
    21361 acgcgcggcg ctcaaccgac gcagggctac gcggtcgtgt tcggcctgcc ggtcaacgtc
    21421 ttcctgctgc cgtggctcgg cgtcggcgtt cgcggtcagg tcgtgtacag ccaggatccc
    21481 gccgccgacg gaccgatgac accgtatcgc agcgacgacg tagagaccga tccactggct
    21541 gcgtggagcg cgacggcggg accgcgcttt aggttctggt ccgatccggc agcacggctg
    21601 cgctgggtag tcgacgtcga cgccggttac gtccacccag atccgatctc gccagcgggt
    21661 ccgctcgcgc ggctgagcct cggccgtcaa gtcggcatct tcaatccgca aggcaacggc
    21721 gcgaacctcg gtgtctcgct cgccgccgaa accggcctcg gtcccgcggc gtcgtatcgc
    21781 gcgttctacg cgagcgcgtg gttccatggc gagtcaggcg tgttcgcggc aaccgaccct
    21841 ggcgacgacg gcgcatggtt tccgtcgaca ttctcgatcg acatgccgat gttgctcggc
    21901 aagaacttcg gcgacgtgcc cggcaatctg atcttcggcg tcgcggccgc gttcggcgtc
    21961 gcggtcagcg acttgctcga gccgcgcatc cggatcgaca cgcactacat ccgacacagc
    22021 gatcaggacg caacgaagac gctcggcgcg tcggccggca cacggctgcg cttcgatcac
    22081 tacggcgccc cggcgttcgt cgatctctac gccggatacg agcacgcgta cggcgtcgaa
    22141 ccgcggctga tcggcaatgg caggttcgtc gacgtcggca ccggcctgca catcgtcgcg
    22201 tgcaagttcg aatatgacct cggcgtgcgc ttccggctcg gcgtcagcga cgacaacagc
    22261 aagctcgcgg acatcgcgct cgtgctcggc ggcgcgcgcg gcaataccgt caccgcgctc
    22321 ggcaaaccga tcggcaagtg cgactagcac ctactgagct cctgattcct cgtcggtagc
    22381 tggtgcgcgc gccgatgcgc agcgcagtgc ccacatcggc tccgacgttt tgtcttcacg
    22441 gtgtcattgg cctgtggccg aatcgtaaga tgaggaactt tgcgctcgtg ctcgtactcg
    22501 tagcgtgctc gagctcgacg acgacaacac cgtcgtcgaa gctcgccatc gatcgcaaca
    22561 acgtgaccga cttcgacgcg accgtgcgcg aaggcccggc gtgggcacgg gtcgtcgtgc
    22621 gctatacgcc gacgacaacg ttctacgagc tctcgacgtc gtacggtttg ctcgcgttcg
    22681 ccggcaccga ggtgaacttc tccgctggcg atccgggcgt cgaccaatcg gttcgcgagc
    22741 tgctcgccga ctcgcgcgcg accgacgacg gctacgtgat cctcgcggcg ggcgatcccg
    22801 aatatgatct cgtcgaagga ttgctcgacc agctgatcgc agccggcgcg gtgcaatcgc
    22861 caacgctcga gcaaaaaggg acgacgctct atgctgccgc gtacacgact gcgcgcgtgc
    22921 tcggcatgca gctcgcgaca cacgcaccgg gcaccggtgg taggcacggc ggcggtgcgc
    22981 acga
//
LOCUS       DASUFX010000221         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1422267, whole genome shotgun sequence.
ACCESSION   DASUFX010000221 DASUFX010000000
VERSION     DASUFX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422267"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFV99_08735"
     CDS             complement(<1..302)
                     /locus_tag="VFV99_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059431.1"
                     /translation="MRVVVPAKVVPPTVGNVVNTKTIYLNRCTGGCKVTVGFTNSRTN
                     TSAIAQQNGTLSAFSYGDTAWNGVVTCVKNVMSRFNVTVTDVDPGPNVDHFEVMVGG"
     gene            complement(500..1882)
                     /locus_tag="VFV99_08740"
     CDS             complement(500..1882)
                     /locus_tag="VFV99_08740"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5059432.1"
                     /translation="MKRTLALSAALALGMAGSSAVAAPRATYKWTDPSLQGTEYKAPN
                     AAAVSHVIYLNDCKPNGCSLHAGYDNATTDTSSIPNSNATVSPYSGSATNWTALVNCV
                     KQTYADFDVEIVTTRPTSGNYHMAIVAGQPTQVGMQNGVLGVSPFSCGYISNSISFTF
                     ANVVPNDVPELCWTVAQETAHSWGLDHKYDNRDPMTYLSGGPNIKSFQNEAGSCGEYS
                     ARTCSCTYAGTGNAKMNSYALIMATFGSNTPDTVAPTVNITYPTEGAQITAGFPVRAD
                     INDDRTVDHAEFRLDGQLVKTLTEAPWNWSAPTTLGQGAHKVEITAYDRGGNTAKQTV
                     NVQNGTVCMAATDCTTAGQVCVDGHCVAGPGMPGGLGSPCTMSTDCASNQCGSDADGN
                     SYCVEACDPSMNACPSGFDCQDAGNGAGVCWPGGGDDGGCSTNTSSSGGAGLLLLGMA
                     AMFITRRRRK"
     gene            complement(1992..3026)
                     /locus_tag="VFV99_08745"
     CDS             complement(1992..3026)
                     /locus_tag="VFV99_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX5059433.1"
                     /translation="MGSILHVGELILGIAILYWGAEWLIRGSAALARVLGVKPLVIGL
                     TVVAYGTSAPELAVATKTALAHSQPIALGTVIGSCAANISLILGLTALIAPPTVDGRI
                     IRREVPVLVGSAIAVPILFRNGVLSELEGALLILCAIIFTIVTLTVSARLDPDAAIAD
                     DEPEPDASSRSSSSMLSSTKAAESGGSIGGRARPRVSRVTAGLMSLVGLVLLVAGSNL
                     FVRGGRGIAAEIGMSERMLGLTVISIGCALPELMGSLVAALRGHAALAVGSVIGSNLL
                     NVFLVLGVTAYLGPIRLGERMHLVDLVGLVAITLLGVLTLRGSRKITRLEGAILVAAY
                     VAFIVCAAIF"
     gene            complement(3054..3698)
                     /locus_tag="VFV99_08750"
     CDS             complement(3054..3698)
                     /locus_tag="VFV99_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5059434.1"
                     /translation="MEKAAHPVPGRRLLFGLLVVFLVAVVPSIVVPTLMCRPEHKALP
                     DLGEVPAFTLTDQTGQPFSDEALRGKVSIVSFIFTRCDTICPVISMKMARIQEKTFDI
                     GRDIKLLSFSVDPNYDTPERLAAYAKKYGADPERWRFVTGPYDKVHSLIEGPFMTSML
                     RVEDRPSGVPDIQHGGYFLLMDTKMHIRGVYDSDIINQLEALMRDARYLVRTSR"
BASE COUNT          655 a         1262 c         1335 g          680 t
ORIGIN      
        1 ccgccgacca tgacctcgaa gtggtcgacg ttgggacctg ggtcgacgtc ggtgaccgtc
       61 acgttgaacc gcgacatcac gttcttcacg caggtgacga cgccgttcca tgcggtgtca
      121 ccgtaggaga acgcggacag cgttccattc tgttgtgcga tcgcgctcgt gttcgtgcgc
      181 gagttcgtga agccgaccgt caccttgcag ccgccggtgc agcggttcag gtagatcgtc
      241 ttcgtgttga cgacgttgcc gacggtcggc ggcaccacct tcgccggtac tacgactcgc
      301 actcctctcg gctcggcgct tgcggtggcg gccaggacaa gcagcgcggc gacaccgaca
      361 aaactccgca tcccccaaag gctaacgcga cgccgcccga aaaataaacc gccaagtcgc
      421 cgaggtcgcg tgggtcagga ctcgctgagt gtctgacccg agcaagcttg gcagcttggc
      481 ggtgtgaaac gtgtgttcgt tacttgcggc gacgccgcgt gatgaacatc gcagccatgc
      541 cgagcagcaa gaggcctgcg ccaccgctcg agctggtgtt cgtgctgcag ccaccgtcat
      601 cgccgccacc cggccagcag acgcccgcgc cgttgccggc atcctggcag tcgaagccgc
      661 tcgggcacgc gttcatcgag gggtcacacg cctcgacgca gtagctgttg ccgtcggcgt
      721 cgctgccgca ctggttcgac gcgcagtccg tgctcatcgt gcacggcgag ccgagaccac
      781 ccggcatgcc cgggccggcg acgcagtgac cgtcgacgca gacctggccg gccgtcgtgc
      841 agtccgtcgc cgccatgcag acggtgccat tctggacgtt gacggtctgc ttcgcggtgt
      901 tgccgccgcg gtcgtacgcg gtgatttcga ctttgtgcgc gccctggccg agcgtcgtcg
      961 gcgcgctcca gttccacggc gcttcggtga gcgtcttgac gagctggccg tcgaggcgga
     1021 actccgcgtg gtcgacggtg cggtcgtcat tgatgtcggc gcgaaccggg aagccggcgg
     1081 tgatctgcgc gccttccgtc gggtacgtga tgttgaccgt cggcgcgacg gtgtcggggg
     1141 tgttcgagcc gaacgtcgcc atgatgagcg cgtagctgtt catcttcgcg ttgccggtgc
     1201 cggcgtacgt gcagctgcac gtgcgcgcgc tgtactcgcc gcaggagccg gcttcgttct
     1261 ggaagctctt gatgttcggg ccgcccgaga gatacgtcat cggatcgcgg ttgtcgtact
     1321 tgtggtcgag gccccacgag tgcgcggtct cctgcgcgac ggtccagcac agctcgggga
     1381 cgtcgttcgg gacgacgttc gcgaacgtga acgagatcga gttcgagatg tagccgcacg
     1441 agaacggcga cacgccgagc acgccgttct gcatgccgac ctgcgtcggc tgaccggcga
     1501 cgatcgccat gtggtagttg ccgctggtcg ggcgcgtcgt gacgatctcg acgtcgaagt
     1561 cagcgtacgt ctgcttcacg cagttgacga gcgccgtcca gttcgtcgcg ctgccgctgt
     1621 acggcgagac cgtcgcgttg ctgttcggga tgctcgacgt atccgtcgtc gcgttgtcgt
     1681 aacccgcgtg caggctgcag ccgttcggct tgcagtcgtt caggtagatg acgtgcgaga
     1741 ccgcggcggc gttcggagct ttgtactccg tcccctgcag gctcggatcc gtccacttgt
     1801 acgtggcacg cggggcagca actgcagaac ttccggccat ccccagagcc agagccgccg
     1861 acaaggcgag agtacgcttc atggccgccg ttgtagacgg accggcggat ctgcaaaaga
     1921 ctggatccgc tggcactagc tcgcgcgatg tgaggcaccg ggtcgcacac aatgcgacct
     1981 cgtgttcgct actagaagat cgcggcgcaa acgatgaatg cgacgtaagc ggccaccaaa
     2041 atggcgcctt ctaacctcgt gattttgcgg gatcctcgca gggtgagaac tcccagcagc
     2101 gtgatcgcaa ccaagcccac gagatcgaca aggtgcatgc gttcgccaag ccggatcggg
     2161 cccaggtagg cggttacccc aagcaccaaa aatacgttga gcaggttcga tccgatcacc
     2221 gagccgaccg ccagcgcggc atgcccgcgc agcgctgcca ccagcgagcc catcagctcc
     2281 gggagtgcgc agccgatcga gatgacggtg agcccgagca tgcgctcgct catgccgatc
     2341 tcggccgcga tcccgcgccc gccgcgcacg aacaggttcg agcccgcgac gagcaggacg
     2401 aggccgacca ggctcatgag gcccgcagtc acccgcgaga cgcgtggccg ggcgcggccg
     2461 ccgatcgagc cgccactctc cgcggccttg gtcgacgaca gcatcgatga cgacgagcgc
     2521 gagctggcgt cgggttccgg ctcgtcgtcc gcgatcgcgg cgtcgggatc gaggcgcgcc
     2581 gagacggtga gcgtgacgat cgtgaagatg atcgcgcaca ggatcagcag cgcgccttcg
     2641 agctcggaga gcacgccgtt gcgaaacagg atcggcacgg cgatcgcgga gccgacgagc
     2701 accggcacct cgcgccggat gatgcggccg tcgacggtcg gtggcgcgat cagcgcggtg
     2761 agaccgagga tcagcgagat gttcgcggcg catgatccga tcacggtgcc gagcgcgatc
     2821 ggctggctgt gtgcgagtgc ggtcttcgtc gccacagcga gctccggtgc gctcgtgccg
     2881 tacgcgacga cggtcaggcc gatgacgagc ggcttcacgc cgagcacgcg agcgagcgcc
     2941 gccgagccac ggatcagcca ctccgcaccc cagtagagga tcgcgatccc gaggatcagc
     3001 tcgccgacgt ggaggatcga gcccaacgct gcgagcttaa cgcaggcgga tcgttagcgc
     3061 gacgttcgga ccaagtagcg cgcgtcgcgc atcagcgctt cgagctgatt gatgatgtcg
     3121 gagtcgtaga cgccgcggat gtgcatcttc gtgtccatca gcaggaagta gccgccgtgc
     3181 tggatatcgg gcacgccgct cggccgatcc tcgacgcgca gcatcgacgt catgaacggg
     3241 ccctcgatga gcgaatggac cttgtcgtac ggcccggtga cgaaccgcca gcgctcggga
     3301 tcggcaccgt actttttcgc gtacgcggcg agtcgctccg gcgtgtcgta attcgggtcg
     3361 accgagaacg acagcagctt gatgtcgcgg ccgatgtcga acgtcttctc ctggatgcgc
     3421 gccattttca tcgagatgac cgggcagatc gtgtcgcagc gggtgaagat gaagctgacg
     3481 atcgagacct tgccgcgcag cgcttcgtcc gagaacggct ggcccgtctg atccgtgagc
     3541 gtgaacgcgg gcacctcgcc gaggtcgggc agcgccttgt gctcgggacg gcacatcagc
     3601 gtcggcacga cgatcgacgg caccaccgcg accaggaaca cgaccagcag cccgaacagc
     3661 agccggcgtc ctggaacggg gtgtgcggcc ttttccacgg cgggaacctg gcagatccgc
     3721 gacgaaattg cacgcgtgtg tcgctgcggc atggtggcgc gcacgtaggt gggcgatccg
     3781 gcatttcgcg gccatccttt ggatatgtgg ctcgcgcatg cgctcacgct gttgcgcatc
     3841 ccgctcgccg tcgtgttctg gctgacctac ggaaattggc gatggtcgct ggtactcgtc
     3901 gtgctcgcgg cggcgagcga tgcactcgat gg
//
LOCUS       DASUFX010000222         7359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_172379,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000222 DASUFX010000000
VERSION     DASUFX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172379"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFV99_08755"
     CDS             complement(<1..195)
                     /locus_tag="VFV99_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059435.1"
                     /translation="MLKAVLALAAVAGCGRFGFGSNEHAVADGASDDGIGDIDGDGSD
                     TGSDGGGGSDSGSDAMPGPNL"
     gene            complement(235..1710)
                     /locus_tag="VFV99_08760"
     CDS             complement(235..1710)
                     /locus_tag="VFV99_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX5059436.1"
                     /translation="MVQALQQRARVRAYILFLLAACSEPASPDLGDMPGDGKGDGPMD
                     GTGTACATTISYATNWIAPANHPAHFDTVDDNVTWDGTCTDDGPNSYATLSNGWRPYF
                     TGNGACAIALDYSDDCGVAPACETRFTYGDAWGHPANHPAQYDNVAGRAFWDGACRAA
                     GSDSAATLSNGWQPHFTGADSCGIAQRWTNCGGLYANSVLAHGCADPGVVRDGDRYIM
                     SCTSGNAANAFPLYVSTDLVTWTAQGHILPSAVRPAWAKSDFWAPEIHKVGAKWLAYW
                     SARGADGKLAIGAASADDPLGPYTALATPIVPATTVGLIDATAFVSGGTAYLAWKEDG
                     NAQNAPTPIRARQLAADGLSVTGATATLITNTLAWEGNLVEGPFVWEHDGSFYLFYSG
                     NAYYDGRYAVGVAKASAPLGPYTKAAAPIVTTGGHWVGPGHNSVVEGPGGDAYLVYHA
                     WETGHVNGPGDSRKLLVDQVVWRDGWPAVPLAPSSSSRPLP"
     gene            complement(1784..2080)
                     /locus_tag="VFV99_08765"
     CDS             complement(1784..2080)
                     /locus_tag="VFV99_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059437.1"
                     /translation="MRKTHVCPKCQHNRILLIDSVPDMGEVSTEIRRLHIAKIFVGES
                     YFGNEKATTAGALTAAMCRSCGYTELYAENPGSVPIDGKYVREVVGPEQKAPYR"
     gene            complement(2093..2881)
                     /locus_tag="VFV99_08770"
     CDS             complement(2093..2881)
                     /locus_tag="VFV99_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059438.1"
                     /translation="MPEAAAAPTTIDRAKLLPRSGIGVDAPWLVIAPPGNPIIRLDAR
                     LLDRVELIGRSPTAPLVASGLATAGVAAAALSLVPGGVFACAGLAYLAHERFSEARRR
                     SRSRDLLLALGDLEVALHVADGPDAAKGISEQLAPYTRDAPITRPEVYEDAKRRMRAQ
                     AEGKGEASARRELEHALLVGNDVVFLRGEYLQVGQVAFRISEVREYALRGQNLPLTGG
                     RLLQAAMGLLVVAAEERAAAGEDLGLLSKRVADYEAWSGHHAGR"
     gene            complement(2884..3984)
                     /locus_tag="VFV99_08775"
     CDS             complement(2884..3984)
                     /locus_tag="VFV99_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5059439.1"
                     /translation="MSLRGSLGTMSAEDVLEWIGRRKLSAPVTFDRRGLVRSLVIEEG
                     QIVWASSNRRDEQLGVILVKSGLVQERALADALEARAETGVPLGKVLLMSGLITEAAL
                     VEILATKIRETVTDVITWTDGQFDVVPRTQPATGVIAQLPIDVCLTVARRRAERMAAI
                     MSILGADDVTFYVPPNVAPPSTHGAENDLVDVAKIWTLGGDRHTAADIAAVFSGERFA
                     VYEKLAEMVQAGQLVIDRRQRERTNSAVELAAGARGRLRNNDRAGALAMASQALHQDP
                     SDSEVRKTFVQAERARVAEVAKNLLSRHRVPKRVKAASTSLGLSEAEVELANRIDGRW
                     DLLSLIRYANLREAEALLAFAHLAEVGVVELG"
     gene            4024..4791
                     /locus_tag="VFV99_08780"
     CDS             4024..4791
                     /locus_tag="VFV99_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5059440.1"
                     /translation="MADAPKSSARSAPPRADVVKPRGVVVDPKVLLAGGAVAVIGLAV
                     LAFFLWMVPNAAAREARAACNGLKATGPAMCDGQPCQLPMEMPNFTAYDHTGKPVDFK
                     QKYRGKVVLLNFWASWCGVCKTEKPKLYQMAQEMSGGDFVVVALASDRTWTDVLLAIV
                     ESLAPQTKLPDGEVSLDQAKQIYGNAMPKGLPFDVLLDPPEGDNNIGQIAASWGIKAV
                     PESALIDRNGQIRAYFVNKRDWESPVAETCLRSVIDE"
     gene            4791..5156
                     /locus_tag="VFV99_08785"
     CDS             4791..5156
                     /locus_tag="VFV99_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059441.1"
                     /translation="MARVLVIDDEPDVVRLIVKVLSGRGHIVQIARDGASALARVAHE
                     PPDVILLDSDLPKIDGAEVCRRLKTDSTTADIPIVMMTSAYIDIYDVDAEGGPDAFVV
                     RPFVREVLANVVERALFKR"
     gene            5169..6134
                     /locus_tag="VFV99_08790"
     CDS             5169..6134
                     /locus_tag="VFV99_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5059442.1"
                     /translation="MSRVPFTILTGWLGAGKTTALNRMLAAPHGRRIAVLVNELGRIS
                     IDTQLIVGRGGDVLELAGGCVCCKVDTKNDLWDGIGDIVARSAPDHVVLETTGIAEPA
                     AILDGLVKVPEAVRERIMPAGVVCVVDAESGAQAVTTREEAREQAAAADRVLIAKLDV
                     ASADAVRATHAVLDEAARDAERAAFPIDDAGALAMTAWVVEPRKLRAWTTRKHDHDHD
                     EHAGHHHGNQLVAVTFADDAPLVGERVMAVIEQLGERLVRAKGFVHLAGEERRGFVER
                     AGVRTSLELRDAWGGDARRSEIVLIGDGLDEAALRRALWACRAAR"
     gene            complement(6188..6613)
                     /locus_tag="VFV99_08795"
     CDS             complement(6188..6613)
                     /locus_tag="VFV99_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059443.1"
                     /translation="MVAAAEIAAAETAAAEIAAAEVMAAAIAVAAEIVAADIMTTDVV
                     MAFVTTDVMRALVTIAAGVEHAEIADRRDHIIVLLAIDAVVANRRPAAGSAIALSLRR
                     GPYQHPEYTEQQPCEQDRHIDLLRETTSSPIYRSAMLAV"
     gene            complement(6920..>7359)
                     /locus_tag="VFV99_08800"
     CDS             complement(6920..>7359)
                     /locus_tag="VFV99_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5059444.1"
                     /translation="DASLTGELPAGPAHADRSNEIRALVAAVRSRITPDLAASPASAR
                     DAATATAGDCTTFALAYAALAAGRGIPTRVVTGVRIDNNRLIRHRWAVSWTGTRWIAI
                     DAAFGAVPAGGNLIALAVHDADDAGLVAGEAALTQVRAAAWAP"
BASE COUNT         1178 a         2402 c         2566 g         1213 t
ORIGIN      
        1 caggttaggc ccgggcatgg cgtcggagcc gctatcgctg ccaccgccgc cgtcgctgcc
       61 ggtgtcactg ccgtcgccat cgatgtcgcc gatgccatca tcggacgcgc catccgcgac
      121 ggcgtgctcg ttcgagccaa acccgaagcg accgcacccc gccacggccg ccagtgcaag
      181 tacagccttg agcacggacg cccagtataa gcgacggccg cgccgcgttg cgggttatgg
      241 cagggggcgc gatgacgacg aaggtgcgag cggcaccgca ggccaaccat cacgccaaac
      301 gacctgatcg acgagcagct tgcgagaatc gcccggtccg ttgacgtgac cggtctccca
      361 cgcgtgatag acgaggtacg cgtcgccgcc cggtccctcg acgacgctgt tgtgtccggg
      421 cccgacccaa tggccgcccg tcgtgacgat cggggccgcc gctttcgtgt acggcccgag
      481 cggcgcgctc gccttcgcga cacccaccgc gtagcggccg tcgtagtacg cgttgccgct
      541 gtagaacaga taaaagctgc cgtcgtgctc ccacacgaac gggccttcga cgaggttgcc
      601 ttcccacgcg agcgtgttcg tgatcagcgt cgcagtcgcg ccagtcaccg acaacccgtc
      661 ggcggcgagc tggcgcgcgc ggatcggcgt cggtgcgttc tgcgcgttgc cgtcctcttt
      721 ccacgcgagg tacgcggtgc cgccgctgac gaacgcggtc gcgtcgatca agcccacggt
      781 cgtcgccggc acgatcggcg tcgcgagcgc ggtgtacggg ccgagcggat cgtcggcaga
      841 cgcagcgccg atcgcgagct tgccgtccgc gccacgcgcg ctccagtacg cgagccactt
      901 cgcgccgacc ttgtgaatct ccggcgccca gaagtcgctc ttcgcccacg cgggtcgaac
      961 cgcgctcggc aagatgtgac cctgcgcggt ccacgtgacg agatccgtcg acacgtacag
     1021 cgggaacgcg ttcgccgcgt tccccgacgt gcacgacatg atgtagcgat cgccgtcgcg
     1081 cacgacgccc ggatcagcgc agccgtgcgc gagcaccgag ttcgcgtaga gcccgccgca
     1141 gttcgtccac cgctgcgcga tgccgcagct atccgcgccg gtgaagtgcg gctgccagcc
     1201 gttcgagagc gtcgccgccg agtcgctgcc cgctgcgcga cacgcgccgt cccagaatgc
     1261 acggcccgcg acgttgtcgt actgcgcggg atgattcgcc gggtgtcccc acgcatcgcc
     1321 gtacgtgaag cgcgtctcgc atgcgggcgc gacgccgcaa tcatcgctgt agtcgagcgc
     1381 gatcgcgcat gcgccgttgc cggtgaagta gggccgccag ccgttcgata gcgtcgcgta
     1441 cgaattcggg ccgtcgtcgg tgcacgtgcc gtcccacgtc acgttgtcgt cgacagtgtc
     1501 gaaatgcgcc gggtgattcg cgggcgcgat ccagttcgtc gcgtagctga tcgtcgtcgc
     1561 acacgcagtg ccggtgccgt ccatcgggcc atcgcccttg ccgtcgccgg gcatgtcccc
     1621 gagatcgggt gaggcaggct cgctacacgc agcgagcagg aacaagatgt acgcgcgcac
     1681 gcgcgcgcgt tgttgcaacg cctgcaccat gcagcgcgca tcgtcatctc gcatgcttgg
     1741 agactcgccg caggactttc gtcctcgcaa gtgcccacaa cgatcagcgg tatggcgctt
     1801 tctgctcggg gccgacgacc tcgcgcacgt acttgccgtc gatcggcacc gagccgggat
     1861 tctcggcgta gagctcggtg tagccgcagc tgcggcacat cgccgccgtg agcgcgcctg
     1921 ccgtcgtcgc cttctcgttg ccgaagtagc tctcgccgac aaaaatcttt gcgatgtgga
     1981 gtcggcggat ctccgtcgag acctcaccca tgtcgggcac gctatcgatc agcaagatcc
     2041 ggttgtgctg gcacttcggg cagacgtgag tcttgcgcat ccacccacgg tatcaccgtc
     2101 cagcgtggtg accgctccac gcttcgtaat cggcgacgcg cttcgatagc aggccaagat
     2161 cctcgccggc ggcggcgcgc tcctcggcag caacgacgag caggcccatc gcggcttgca
     2221 acaagcgccc gccagtgagc ggcaggttct ggccgcgcag cgcgtactcg cggacctcgc
     2281 tgatccggaa cgcgacctga ccgacctgca gatactcgcc gcgcaagaac acgacgtcgt
     2341 tgccgacgag cagcgcgtgc tcgagctcgc gacgcgcgct cgcttcgcct ttgccttcgg
     2401 cttgtgcgcg catgcggcgc ttcgcgtcct cgtagacttc agggcgcgtg atcggtgcgt
     2461 cgcgcgtgta tggcgcgagc tgctcggaga tgcccttcgc ggcgtcggga ccgtcggcga
     2521 cgtggagcgc gacctcgaga tcgccgagcg cgaggagcag gtcgcggctg cgcgagcggc
     2581 ggcgcgcttc ggaaaagcgt tcgtgggcga ggtaggcgag gccggcgcag gcgaacacgc
     2641 cgccgggcac gagcgagagc gcggcggcgg cgacaccggc ggtcgcgaga ccggaggcga
     2701 cgagtggcgc ggtcggcgag cggccgatca gctcgacgcg atcgagcaga cgcgcatcga
     2761 ggcgaatgat cggattgccg ggcggcgcga tcacgagcca cggcgcgtcg acgccgatgc
     2821 ccgagcgcgg caagagcttc gcgcgatcga tcgtggtggg ggcggctgct gcttcaggca
     2881 tggttatccg agctcgacaa cgccgacctc ggcgagatga gcgaatgcga gcaaggcttc
     2941 tgcttcgcgg aggttggcgt agcgaatcag cgagagcaga tcccaacggc catcgatgcg
     3001 gttcgcgagc tcgacctcgg cttcggagag gccgagcgat gtggaggctg ctttcacgcg
     3061 cttgggcaca cgatgccgcg aaagtaggtt cttggcgacc tctgcgacac gcgctcgttc
     3121 ggcctgaacg aacgtcttgc ggacttcgga gtcgctcggg tcttgatgaa gcgcttgcga
     3181 ggccatggcg agcgcgcctg cacgatcgtt gttgcgcagg cgaccgcgcg caccggcggc
     3241 gagctcgacc gccgagttcg tgcgctcgcg ctggcggcga tcgatcacga gctgaccggc
     3301 ctggaccatc tcggccagct tctcgtagac cgcgaagcgc tcgcccgaga acacggcggc
     3361 gatatcggcc gcggtgtggc gatcgccgcc gagcgtccag atcttggcga catcgacgag
     3421 gtcgttctcg gcgccgtggg tcgatggcgg cgcgacgttc ggcggcacgt agaacgtgac
     3481 gtcatcggcg cccaggatgc tcatgatcgc ggccatccgt tcggcgcggc ggcgagcgac
     3541 cgtcaggcag acgtcgatcg gcagctgggc gatcacgccg gtggccggct gggtgcgcgg
     3601 aacgacgtcg aactgcccgt ctgtccacgt gatcacgtcg gttacggtct cgcggatttt
     3661 cgtggcgaga atttccacca gggcagcttc ggtaatcagc ccagacatga gcagcacttt
     3721 gcctagcggc acgccggtct cggcgcgcgc ctcgagcgca tcagcgagtg cgcgctcctg
     3781 gacgagcccg ctcttgacca gaatgactcc gagttgctcg tcgcggcgat tactgctcgc
     3841 ccacacgatc tggccttctt cgataaccag actgcgtacg agaccgcggc gatcgaatgt
     3901 gaccggggcc gacagcttgc gtcggccgat ccattcgagc acgtcctcag cggacatggt
     3961 gccgagcgaa ccgcgcagcg acatcccttt gagcatagcg gaagttccgg tacattgtcc
     4021 gtgatggccg acgcgccgaa atcgtctgca cgaagcgcac ctccgcgcgc agacgtggtc
     4081 aagccgcgtg gtgtcgtcgt cgatccgaag gtcctactcg cgggtggcgc ggtagcggtg
     4141 atcggcttgg cggtgctcgc gtttttcctg tggatggtgc cgaatgcggc ggcgcgcgag
     4201 gcgcgggccg cgtgcaatgg gctcaaggcg acagggccgg caatgtgcga tggccagcca
     4261 tgtcagctgc ccatggagat gccgaacttc accgcgtatg accacaccgg caagccggtc
     4321 gacttcaagc agaagtaccg gggcaaggtc gtgctgctga acttctgggc ctcgtggtgc
     4381 ggcgtctgca agaccgagaa gccgaagctc tatcagatgg cgcaggagat gagcggcggc
     4441 gacttcgtcg tggtcgcgct cgcgtccgat cgcacctgga ccgacgtgct gctcgcgatt
     4501 gtcgagtcac tggcgccgca gacgaagctg cccgacggcg aggtctcgct cgatcaggcc
     4561 aaacagatct acggcaatgc gatgccgaag ggcctgccgt tcgacgtgct gctcgatccg
     4621 cccgagggcg acaacaacat cggtcagatc gcggcgtcgt ggggcatcaa ggcggtgccc
     4681 gagtcggcgc tcatcgatcg caacggccag attcgcgcgt acttcgtcaa caagcgggac
     4741 tgggagtcgc cggtggcgga gacgtgtctt cgctcggtga tcgacgagta atggctcgcg
     4801 ttctggtgat cgatgacgag ccggatgtcg tgcggctgat cgtgaaggtg ctgtcggggc
     4861 gcggccacat cgtgcagatt gcccgcgatg gcgccagtgc gctcgcgcgg gtcgcgcacg
     4921 agccgcccga tgtcattctg ctcgacagcg acctgccgaa gatcgacggt gccgaggtgt
     4981 gtcgccggct caagaccgac tcgacgaccg cggacatccc gatcgtgatg atgacgtcgg
     5041 cctatatcga catctacgac gtcgacgccg agggtggccc ggatgcgttc gtcgtgcggc
     5101 cgttcgtgcg cgaggtgctc gcgaacgtcg tcgagcgcgc gctgttcaag cgctgagggc
     5161 aggttggcgt gtcgcgtgtt ccattcacga tcctgaccgg ctggcttggc gccggtaaga
     5221 caacggcgct caaccggatg ctcgccgcac cgcacggccg ccgcatcgcg gtgctcgtca
     5281 acgagctcgg ccgcatctcg atcgatacgc aattgatcgt cggtcgcggt ggcgacgtgc
     5341 tcgagctcgc cggcggctgt gtgtgctgca aagtcgatac gaagaacgat ctgtgggacg
     5401 gcatcggcga catcgtcgcg cgctcggcac ccgatcacgt cgtgctcgag acgaccggca
     5461 tcgccgagcc cgccgcgatc ctcgacggcc tcgtcaaggt gcccgaagca gtgcgcgagc
     5521 gcatcatgcc tgccggcgtc gtgtgcgtcg tcgatgccga gagcggcgcg caggccgtga
     5581 cgacgcgcga agaagcgcgc gagcaagcag ccgctgccga tcgcgtgctg atcgcgaagc
     5641 tcgacgtcgc gagtgccgat gcggtacgcg cgacgcacgc ggtgctcgac gaggctgcgc
     5701 gcgatgccga acgcgccgca tttccgatcg acgatgcggg cgcgctcgcg atgaccgcgt
     5761 gggtcgtcga gccgcgcaag ctgcgtgcgt ggacgacgcg gaagcacgac cacgatcacg
     5821 atgaacacgc cggccatcac cacggcaacc aactcgtcgc cgtgacgttc gccgatgatg
     5881 cgccgctcgt cggtgagcgc gtgatggccg tgatcgaaca gctcggcgag cgactcgtgc
     5941 gcgcgaaagg cttcgtgcat ctcgccggcg aagagcgtcg cggcttcgtg gagcgcgcag
     6001 gcgttcgcac gtcactcgag ctgcgcgatg cgtggggcgg tgatgcgcgg cgcagcgaga
     6061 tcgtgttgat cggcgatggt ctcgatgaag ctgcgttacg gcgcgcgctg tgggcgtgcc
     6121 gtgcagcgcg ttaattgacg caaattgacg cagagagacg cgccgactcc gagagtttgc
     6181 cgtcccgtca cacagcgagc attgccgatc ggtaaatcgg tgacgaggtt gtttcgcgaa
     6241 ggaggtcgat atgtcgatct tgttcgcagg gctgttgctc ggtgtattcg ggatgctggt
     6301 atgggccgcg ccgaagcgaa agcgctatcg ccgatcccgc cgctggacgc cggttggcga
     6361 cgacagcatc gatggcgagg agaacgatga tgtggtctcg acggtcagcg atctcggcgt
     6421 gttcaactcc ggcggcgatc gtcacgagag ctcgcatcac gtcggtggtc acgaaggcca
     6481 tcaccacgtc ggtggtcatg atgtcggcgg ccacgatttc ggcggccacg gctatggcgg
     6541 cggccatgac ttcggcggcg gcgatttcgg cggcggcggt ttcggcggcg gcgatttcgg
     6601 cggcggccac cactagcgcg cacgctcgac ggctcgcgcg catcactcac caggcgcgcg
     6661 acgcggctta gtcgtcgctc cagtcgacga cgtcgtcgac ctggcgtccg cgtgccggca
     6721 ggaacaggag cacgacgaag accgctgctg ctgctgcgta gatgattgcc tccatcgtga
     6781 cctcctcgtg agaaacctgg ccacgttcgc aggcgtgcgc aaggaaaagt ttgcacagct
     6841 ccgacgatgt caccctcgcg ccacatacga ggcgtcgctc agcgtcaacg atcagctgtg
     6901 ctggcgatat tcccgagcgt cagggcgccc acgcggcggc ccgcacctgg gtgagcgccg
     6961 cttcgccggc aacgagtccg gcgtcgtcgg catcatgtac ggcgagcgcg atgagattgc
     7021 cgccggcggg tacggctccg aacgcggcgt cgatcgcgat ccagcgggtg ccggtccagc
     7081 tgaccgccca ccggtgtcgg atcaaccggt tgttatcgat gcgaacgccg gtgacgaccc
     7141 gcgtcggaat cccgcggcca gcggcgagcg cggcgtaggc gagtgcgaac gtcgtgcagt
     7201 cacccgcggt cgcggtcgcg gcatcgcggg ccgaagccgg cgacgcggcg aggtcgggcg
     7261 tgatgcgtga gcgcacggcg gcgacgagcg ccctgatctc gttgctgcga tcggcgtggg
     7321 ccggacctgc ggggagctcg ccggtcagag aagcatcga
//
LOCUS       DASUFX010000223         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_33655,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000223 DASUFX010000000
VERSION     DASUFX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33655"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VFV99_08805"
     CDS             <1..1263
                     /locus_tag="VFV99_08805"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827886.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5059445.1"
                     /translation="EDFADACTAAGITFIGPSADSMRKMGSKTEARKTVMAAGTPVVP
                     GDNGPTGDGFPSAEVALPAAKKIGFPVLIKAAAGGGGKGMRLVANEHDFPGAFDGAVR
                     EATAAFGDGTVYLEKAIERPRHIEIQVFGDAHGNLVYLGERDCSIQRRHQKVIEEAPS
                     PVVTADLRKRMGEAAVAAARAVKYLGAGTCEFLLGADGGFYFLEMNTRLQVEHPVTEM
                     IYGVDLVAWQLEVAEGKPLPMTQAELDARRRGHAIECRIYAEDPVKFLPSPGRITHLR
                     VPDGPYVRNDSGCYEGAEIPVHYDPMISKLVVWGEDRKAALARMRRALDEYAVRGIET
                     NLAFHRRCLRHAAFIAGDYDTGFIARNAAELAPKADEAELDAAIIAAALEAHTQSHAT
                     QKPTTSANGKAVAHGATTEISGWRQQLR"
     gene            1260..1655
                     /locus_tag="VFV99_08810"
     CDS             1260..1655
                     /locus_tag="VFV99_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059446.1"
                     /translation="MSVTCAFCEDAATNNCRRCEGPLCGMHKPWKPELACITCEEEWE
                     RGRTKRTLILFPITLAALGVGMGLDGLFVWATEHFSVAPGGWGFVLLFFAVPIMIAIG
                     VVRWVTKQTFRRRFFAHKPRRVPRAEVVK"
     gene            1776..2153
                     /locus_tag="VFV99_08815"
     CDS             1776..2153
                     /locus_tag="VFV99_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059447.1"
                     /translation="MSRLLTVLLFATVTTAACTSTPSGAACPDNPTDVPTYDNFGREF
                     METYCTGCHSAKSPDRHGAPGDQNYDTEADIKTHAAAIDAEAAAGPNATNTAMPEIAG
                     TVHAAPTQAEREMLGKFLACEQK"
     gene            2341..3951
                     /locus_tag="VFV99_08820"
     CDS             2341..3951
                     /locus_tag="VFV99_08820"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5059448.1"
                     /translation="MAQQTKFVFVTGGVVSSLGKGMVSATIGALMENRGLKVANMKLD
                     PYINVDPGTMNPTQHGEVYVTDDGAETDLDLGHYERFVSTKMSRLNNITTGQIYSSVI
                     GKERRGEYLGSTVQVIPHITDEIKSRILRCAEGLDVLVVEVGGTVGDIESLPFLEAVR
                     QLRVELGLQNSVSVHLTLVPHIRAAGEFKTKPTQHSVQKLREIGIQCDVLIVRCEQKL
                     DESVIKKMAMFCNVAADSVFQSIDVDTVYRLPIALSEQGLDQKLAAILNIWSRASRLS
                     AWEEVVKRVTEPKRKVTVGFIGKYVSLVEAYKSLNEALLHGGIANDCRVEIKHIDSED
                     LERTGTASLAEYDGILVAPGFGARGTEGKIAAVRYAREKQVPYFGICFGMQMACIEYA
                     RHVCGLDKANSSEVDPSTPHPVVDLMPDQRGVTDKGATMRLGAYPCVLKAGTRAADAY
                     GSTEISERHRHRWEINNNYRDTLERHGMVLSGLSPDGRLVEMIELPQHPYFVACQFHP
                     EFKSRPSAPHPLFARFIKASVEHQAKRR"
     gene            4321..>4589
                     /locus_tag="VFV99_08825"
     CDS             4321..>4589
                     /locus_tag="VFV99_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX5059449.1"
                     /translation="MGMEMRQELKLSQQLVMTPQLQQAIRLLQLSRMELADLVHEEML
                     ENPILDDEMDLDNERSRERDEMGGDEQALHQLESAGSTETPVEGSV"
BASE COUNT          840 a         1502 c         1490 g          757 t
ORIGIN      
        1 gaagatttcg cggacgcgtg cacggcagcc ggcatcacgt tcatcggtcc gagcgccgac
       61 tcgatgcgca agatggggag caagaccgaa gcgcgaaaga ccgtgatggc cgcgggcacg
      121 cccgtcgttc cgggcgacaa cggaccgacc ggcgacggct ttccgagcgc ggaggtcgcg
      181 ctgcctgccg cgaagaagat cggctttcca gttctcatca aggcagcggc aggtggcggc
      241 ggtaagggca tgcgtctcgt cgcgaacgag cacgacttcc ccggtgcgtt cgacggcgcg
      301 gttcgcgaag cgaccgccgc attcggcgat ggcactgtct atctcgagaa agcgatcgaa
      361 cgtccgcgcc acatcgagat ccaggtgttc ggcgacgcgc acggcaacct cgtctatctc
      421 ggcgagcgcg actgctcgat ccagcgccgt caccagaagg tgatcgaaga agcgccgtcg
      481 ccggtcgtca ccgcggacct gcgcaagcgc atgggcgaag ccgcggtcgc cgccgcgcgc
      541 gcggtgaagt acctcggcgc cggcacgtgc gagttcctgc tcggtgccga cggcggcttc
      601 tacttcctcg agatgaacac gcgcctgcag gtcgagcacc cggtcaccga gatgatctac
      661 ggcgtcgatc ttgtcgcgtg gcagctcgag gtcgccgaag gcaagccgct accgatgacg
      721 caggccgagc tcgacgcgcg ccgccgcggt cacgcgatcg agtgccgcat ctatgccgag
      781 gatccggtga agttcttgcc gtcgccgggc cgcatcacgc acctgcgcgt gcccgatggc
      841 ccgtacgtcc gcaacgacag cggctgctac gagggcgccg aaatcccggt gcactacgat
      901 ccgatgatct cgaagctcgt cgtgtggggc gaagatcgca aggcagcact cgcgcgcatg
      961 cgccgcgcgc tcgacgagta cgcagtgcgc ggcatcgaga caaacctcgc gtttcaccgc
     1021 cggtgcctcc gccacgctgc gttcatcgcc ggcgattacg acaccggctt catcgcacgc
     1081 aacgcagccg agctcgcgcc caaggccgac gaggccgagc tcgacgcggc gatcatcgct
     1141 gcggcgctcg aagcgcacac gcagtcacac gcgacgcaga agccgacgac gtccgcgaac
     1201 ggtaaagcgg tcgcgcacgg tgccaccacc gagatctccg gctggcgcca gcagctgcga
     1261 tgagcgtgac ctgcgcgttc tgcgaggacg cggccacgaa caactgtcgc cgctgcgagg
     1321 gcccgttgtg cgggatgcac aagccgtgga agcccgagct cgcatgcatc acgtgcgagg
     1381 aggagtggga gcgcggccgc acgaaacgaa cgctgatcct gttcccgatc acgctcgccg
     1441 cgctcggcgt cgggatgggg ctcgacggcc tgttcgtttg ggcgaccgaa catttctcgg
     1501 tcgcgccggg cggctggggc ttcgtcctgc tgttctttgc ggtgccgatc atgatcgcga
     1561 tcggcgtggt tcgctgggtc accaagcaga cgtttcgccg gcggttcttc gcgcacaagc
     1621 ctcggcgtgt accgcgcgcc gaagtggtga agtgaccgtc gcccgcgcgg tggaacttcg
     1681 acgcagaccc agctcgacct ccgacgctga atcgcgcacc gccgcgaaat cgcctgctgt
     1741 gtgggctggt ctgcgagttg ctgttgtcga gcgacatgtc tcgactgctt accgtgctgt
     1801 tgtttgccac cgtcaccacc gctgcttgta cgtcgacgcc atcgggcgcc gcgtgccccg
     1861 acaatccgac cgacgttccg acgtacgaca actttggccg cgagttcatg gagacgtatt
     1921 gcaccgggtg tcactcggcg aagtcgccgg atcgccatgg cgcgcccggc gatcagaact
     1981 acgacaccga agcggacatc aagacgcacg ccgccgcgat cgatgcggag gccgcggccg
     2041 gcccgaacgc gacgaacacc gcgatgcccg agatcgccgg cacggtgcac gcggcgccga
     2101 cccaagccga gcgcgagatg ctcggcaagt tcctcgcgtg cgagcagaaa taagtcatgg
     2161 cgcacctagt cgatcggggt ggcgcaaggg agatcttgcc gcccccgacg acgaatcgac
     2221 ggccggccga tcgcgcgggt ctctgatttt cctaggcgat ctcgacctat tgtttttcgt
     2281 catagcgatc aggtagaaac cgtctggcgc acttcatgtc accgcgccac gaggattctc
     2341 atggcgcagc agacgaagtt cgtgttcgta accggcggtg ttgttagctc gctcggcaag
     2401 ggcatggtct cggcgacgat cggtgcgctg atggagaacc gcggcctcaa ggtcgcgaac
     2461 atgaagctgg acccgtacat caacgtggat ccaggcacga tgaacccgac gcaacacggc
     2521 gaggtctacg tcaccgatga cggtgccgag accgacctcg acctcggaca ctacgagcgc
     2581 ttcgtctcca cgaagatgtc gcggctcaac aacatcacga ccggtcagat ctactcgagc
     2641 gtgatcggca aggagcgccg cggcgagtac ctcggttcga ccgttcaggt cattccgcac
     2701 atcacggacg agatcaagag ccggatcctg cgctgtgccg aaggcctcga tgttctggtc
     2761 gtcgaagtcg gtggcacggt cggcgacatc gaatcgctgc ccttcctcga agcggttcgt
     2821 cagctgcgcg tcgagctcgg cttgcagaac tcggtcagcg tccacctcac gctcgtgccg
     2881 cacattcgcg cggccggcga gttcaagacc aagccgaccc agcactcggt ccagaagctg
     2941 cgcgagatcg gcatccagtg tgacgtgctc atcgtgcgct gcgagcagaa gctcgacgag
     3001 agcgtgatca agaagatggc gatgttctgc aacgtcgccg ccgacagcgt gttccagtcg
     3061 atcgacgtcg acaccgtcta ccggctgccg atcgcgctga gcgagcaggg cctcgatcag
     3121 aagctcgccg cgatcttgaa catctggtcg cgcgcgtcac ggctcagcgc gtgggaagag
     3181 gtcgtcaagc gcgtcaccga gcccaagcgc aaggtgacgg tcggcttcat cggcaagtac
     3241 gtatcgctcg tcgaggcgta caagtcgctc aacgaagcgc tgctccacgg cggcattgcg
     3301 aacgactgcc gcgtcgagat caagcacatc gacagcgagg acctcgagcg caccgggacg
     3361 gcgagcctcg ccgagtacga cggcatcctc gtcgcgcctg gcttcggtgc gcgcggtacc
     3421 gaaggcaaga ttgcggcggt gcgctacgcg cgcgagaagc aggtcccgta cttcggcatc
     3481 tgcttcggca tgcagatggc ctgcatcgag tacgcgcgcc acgtgtgcgg gctcgacaag
     3541 gcaaactcga gcgaggtcga cccgtcgact ccgcatcccg tcgtcgatct catgcccgat
     3601 cagcgcggcg tcaccgacaa gggcgcgacg atgcgcctcg gcgcgtaccc gtgcgtgctc
     3661 aaggccggca cgcgcgcggc cgacgcctac ggctcgaccg agatcagcga gcgccaccgc
     3721 catcgctggg agatcaacaa caactatcgc gacaccctcg agcgccacgg catggtgctg
     3781 tccggtctgt cgccggacgg gcggctagtc gagatgatcg agctgcccca gcacccgtac
     3841 ttcgttgcct gccagttcca ccccgagttc aagtcgcgcc cgagcgcacc gcacccgctg
     3901 ttcgcgcgct tcatcaaggc gtcggtcgag catcaagcca agcgccgctg atcgcagaac
     3961 ctggtctgcc cccatttcgc gtcgctgatc aggcttcggc tgagaaccgg ggtctgaccc
     4021 atctggtttc gtccgccacg cggacgcaat cgctgctgca aaggctgcag tgggcggtcc
     4081 ggcacggcct cgatccgggt gcacaggggc gtttgtggcg gcttcgctcg atcgcagctc
     4141 caaaaatcga tccactttgt gcttgacggt gaggggtcgg ttagggtccg agcaagagcc
     4201 gggccatcac cctttacagc ccccttgttt caccgtacgc ttggagtaat tcaatcgtcg
     4261 taccaggtga tggccagcag ggcgtttact ttaactagct gagctccaag gagaatctcg
     4321 atgggcatgg agatgcgcca ggaattgaag ctgtcgcagc agctggtgat gacaccgcag
     4381 ctgcaacagg cgatccggct tcttcagctg agccgcatgg agctggccga cctcgtgcat
     4441 gaggagatgc tcgagaaccc catcctcgac gacgagatgg atctcgataa cgagcgcagc
     4501 cgcgagcgcg acgagatggg tggggacgag caagcgctcc accagctcga atctgctggt
     4561 tcgaccgaga cgccggtcga agggtcggt
//
LOCUS       DASUFX010000224         4794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1007481, whole genome shotgun sequence.
ACCESSION   DASUFX010000224 DASUFX010000000
VERSION     DASUFX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007481"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFV99_08830"
     CDS             complement(<1..890)
                     /locus_tag="VFV99_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5059450.1"
                     /translation="MTKAKAGGEQPVEIPDTLPILPLRNSVLFPGAIIPIDVGRRKSV
                     RLVEDAIAKERPVIGILTQKDARTEDPGAGDLYMVGCAARILKVIKLAKDNFSVILQG
                     VSRFEVSTFDGAEPFLSAKVRSVPDPTSSDVELDALVMNLKDIAKRVVKLMPELPKEA
                     GALVDSVTEAGHLADLITSHLELEVGEKQDVLETFDLKTRTRKVLQFLSRQLEVLKVR
                     ERINTQVQEEMGRNQREYVLRQQLKAIKEELGELDDGGGDLDEFSEKITKAKMPEEAE
                     KVAKKQLDRLKGMQPSSAEYT"
     gene            complement(961..1236)
                     /locus_tag="VFV99_08835"
     CDS             complement(961..1236)
                     /locus_tag="VFV99_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059451.1"
                     /translation="MRARLRCPACGNARLAHAKQVLDRGDGDSRKVMALAQPSWWSDR
                     LVGELEAFVCTACGLVEWYVKDPGRLREVKGQITILDGETPESGPYR"
     gene            complement(1390..1788)
                     /locus_tag="VFV99_08840"
     CDS             complement(1390..1788)
                     /locus_tag="VFV99_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059452.1"
                     /translation="MRFGFALWIVLVLCVSCGNKQEKVTREQCAKVADHIADLIIDHY
                     AAHPDELWEAVTSQPGETGVPPTVTKETFKAFLDTPEGKTWLMQRHGQARSGTESGIP
                     DCVAKATPKLVSCLLAAKSRDDVTACDQAK"
     gene            1881..2615
                     /locus_tag="VFV99_08845"
     CDS             1881..2615
                     /locus_tag="VFV99_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5059453.1"
                     /translation="MSPIPKADPNATTAIDQVFEKLLSDIVSGVHPAGSRLPAERELS
                     RQLGASRPTLREALRRLGEWSLVEPRRGSGVVVRPYRDWSIEVLAAYLRYGKPEQGQP
                     TVGRILIDMLGMRRAVVLEVIKLTAARVPKGGTAGARAAMARAWSLRSTPAFAREDFE
                     VMRQMAEAARFTPGVWLLNRIAKLWADAAGEVAFAVGLPEDYIPVYTKFFDLVEAGSA
                     DEACKHMAEYFERHDATITKALAVLG"
     gene            2612..3073
                     /locus_tag="VFV99_08850"
     CDS             2612..3073
                     /locus_tag="VFV99_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059454.1"
                     /translation="MNAASKRVLHSLVPVICPPDAEPLADAIVEHMELTLAASGPMIG
                     KAFDAGLLAYDLGAVARYGRRAHALTGDRAERYYQYWENTQFGRAINQIMSLACYEQP
                     EMMRAVGFEPEPWIAEVTKKRLTVYADDVRKQDAQILAPDPLRPLRKKEVA"
     gene            3073..4626
                     /locus_tag="VFV99_08855"
     CDS             3073..4626
                     /locus_tag="VFV99_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5059455.1"
                     /translation="MAVARPALHEGARPAGVFTRHDIHGDTVLDADIVIIGSGAGGAT
                     MAAELAEAGFEVIVLEEGSYYATRDFTANSSGMIRQLYRDGGATMALGRPPILYQEGR
                     AVGGSTVVNGGMSWRTPEKILERWQGMGLDTSPKEMEQYFARVEKRIHVAPMDADAIG
                     NDNKLLKKGADAKGWEIIGNLRNQAHCVGSNRCAFGCPTGAKQSALVSYVPRALHYGA
                     RVYADVKVERITRHGKRATGVIGHVRHPDGRRGHNVAVRAKLVVAACGAIHTPALLVR
                     SGFRSPSGRIGHNLSMHPNVKVIAIMDSQVTGWKGAHQAYQVREFQDQGLVFASINLP
                     PSLMAMSLPHRGRALGELMGQYDHLMLAGMLCEDTATGRVRTIDGKPQAFYQLNDHDA
                     ANLQRGVSLLSELLFAAGATRIMLPFHGAADLCSPDDARRLLDQKIAASGWEVLTVHM
                     MGTAAMGTDRLSAVTDQFGQVYDADRLIVADASLFPTPIGVNPMETIMALATRCAAHV
                     IDNAGRLLS"
BASE COUNT          800 a         1602 c         1539 g          853 t
ORIGIN      
        1 gtgtactccg cgctcgacgg ctgcatgccc ttgaggcgat cgagctgctt cttggcaacc
       61 ttctcggcct cctcgggcat cttcgccttc gtgatcttct cggagaactc gtcgagatct
      121 ccgccgccgt catcgagctc accgagctct tccttgatcg ccttgagctg ttggcgcagc
      181 acgtactcgc gctggttgcg ccccatctcc tcttgcacct gcgtgttgat gcgctcgcga
      241 accttgagca cttcgagctg gcgcgacagg aactggagca ccttgcgggt gcgcgtcttg
      301 agatcgaacg tctcgaggac gtcctgcttc tcgccgacct cgagctcgag gtggctcgtg
      361 atcagatcgg cgaggtgacc cgcttcggtc acgctgtcga cgagcgcgcc ggcttccttc
      421 ggcagctccg gcatgagctt cacgacgcgc ttcgcgatgt ccttcaggtt catcacgagt
      481 gcatcgagct cgacatcgct cgacgtcgga tccggaacgc tacgtacttt tgcagacaag
      541 aacggctcgg caccgtcgaa cgtcgacact tcgaagcgcg agacgccctg caagatgacg
      601 gagaagttgt ccttcgcgag cttgatgacc ttcagaatcc gcgcagcgca gccgaccatg
      661 tagaggtcgc cggcgccggg atcttcggtg cgcgcgtctt tctgcgtgag gatgccgatg
      721 accggccgct ccttcgcgat cgcatcctct acgagacgta ccgactttcg ccggccgaca
      781 tcgatcggga ttatggcccc cggaaacagc acggagttgc gcagcgggag aatggggagc
      841 gtgtccggaa tctccaccgg ttgctctccc cctgccttgg ccttcgtcat ggtcgaaacc
      901 tagctagctg gatccaggag cgtcaaggcg accggaggtt attggcgcct aaacgattgg
      961 ctagcgatac ggtccagatt ctggcgtttc gccgtcgagg atcgtgatct gccccttgac
     1021 ctcacggaga cgtccagggt ctttcacgta ccactcgacc aggccgcaag ctgtgcacac
     1081 gaacgcctcc agctcaccga cgagcctgtc ggaccaccaa cttggctggg cgagcgccat
     1141 caccttgcgc gagtcaccgt caccgcgatc cagcacttgc ttcgcgtgcg cgagccgggc
     1201 gtttccgcac gctggacagc gcagccgagc ccgcatggtc ggcgcctgat gtaggcgtgc
     1261 gctgtcgagt tgcgcacgga gctggcctac ctcggcgcgg agctccgcaa gctcccgtag
     1321 aagagtgtcg tccgccatcg cggcgcagca tcacacgccg tccgacgagc cgtggcgcaa
     1381 accagcttgt tacttcgcct gatcgcacgc ggtgacgtcg tcgcgtgact tcgcggcgag
     1441 cagacagctg accagtttcg gcgttgcctt cgcgacgcag tcggggatgc ccgattcggt
     1501 gccgctgcgc gcctgaccat ggcgctgcat gagccacgtc ttgccttcgg gcgtgtcgag
     1561 aaacgctttg aacgtttcct tcgtcaccgt cggtggaaca ccggtctcgc cgggctggct
     1621 cgtgaccgct tcccagagct cgtcggggtg cgccgcgtag tgatcgatga tgaggtcggc
     1681 gatgtggtca gccaccttcg cgcactgctc gcgggtgacc ttctcttgct tgttgccaca
     1741 actgacgcag agaacgagca cgatccacaa cgcgaagccg aagcgcatcc tgccgacctt
     1801 acaccgaaac ttcgtgcttg actcgaacac atgattggtc ttaccaattg gtcataccga
     1861 aatcctccga ggccgcaacc atgtcgccga ttcccaaagc cgatccgaac gccaccaccg
     1921 ccatcgatca agtgttcgag aaactgctct cggacattgt cagtggcgtc caccctgccg
     1981 gatcgcgctt gcccgcggag cgcgagctct cgcgacaact cggtgcatcg cggcccacgc
     2041 tacgtgaagc gctcaggaga ctcggagagt ggagcctcgt cgagccgcgt cgcggttcgg
     2101 gcgtggtggt gcgtccgtat cgcgactggt cgatcgaagt gctcgcggcg tacttgcggt
     2161 acggcaaacc cgagcaaggc caaccgacgg tcggccgcat tctgatcgac atgctcggca
     2221 tgcgccgcgc ggtcgtgctc gaggtcatca agctgaccgc ggcgcgcgtt ccgaagggcg
     2281 gcaccgctgg tgctcgcgcc gcgatggcgc gcgcgtggtc gctgcgctcg acgcccgcgt
     2341 ttgcccgcga ggatttcgag gtgatgcgac agatggccga agccgcgcgg ttcacgcccg
     2401 gcgtgtggct gctcaatcgg atcgcgaagc tgtgggccga cgccgccggc gaggtcgcat
     2461 tcgcggtcgg tctgcccgag gactacatcc ccgtgtacac caagtttttc gatctcgtcg
     2521 aagcgggcag cgctgacgag gcgtgcaagc acatggcgga gtacttcgag cgccacgacg
     2581 cgacgatcac caaggcgctg gcggtgctcg gatgaacgcc gcgtcgaagc gcgtgctgca
     2641 ttcgctcgtg ccggtgatct gcccgcccga cgcggagccg ctcgccgacg cgatcgtcga
     2701 gcacatggag ctcacgctcg ccgcgtcggg cccgatgatc ggcaaagcat tcgacgccgg
     2761 cctgctcgcg tacgatctcg gcgcggtcgc gcgctacggt cgtcgcgcgc atgcgctgac
     2821 cggcgatcgc gccgagcgct actaccagta ctgggagaac acgcagttcg ggcgcgcgat
     2881 caatcagatc atgagcctcg cctgctacga gcagcccgag atgatgcgcg cggtcggctt
     2941 cgagcccgag ccgtggatcg cagaagtcac gaagaagcgg ctcacggtgt acgccgacga
     3001 cgtgcgcaag caagacgcgc agatcctcgc gcccgatccg ctgcgcccgc tccgcaagaa
     3061 agaggtggcg tgatggccgt cgctcgccct gccctgcacg aaggtgcgcg ccccgcgggc
     3121 gtgttcacgc gccacgacat ccacggcgac accgtgctcg atgccgacat cgtcatcatc
     3181 ggctctggcg ccggtggcgc gacgatggcc gccgagctcg ccgaggctgg tttcgaggtg
     3241 atcgttctcg aagaaggttc ctactacgcg acgcgcgact tcaccgcgaa cagctcgggg
     3301 atgattcgtc agctgtatcg cgatggcggc gcgacgatgg cgctcggccg cccgccgatc
     3361 ctctatcaag agggccgcgc ggtcggtggc tcgaccgtcg tcaacggcgg catgtcgtgg
     3421 cgcacgccgg agaagatcct cgagcgctgg caaggcatgg gcctcgacac gtcaccgaag
     3481 gagatggagc agtacttcgc acgcgtcgag aagcgcatcc acgtcgcgcc gatggatgcc
     3541 gacgcgatcg gcaacgacaa caagctgctc aagaagggcg ccgacgcgaa gggctgggag
     3601 atcatcggca acctgcgcaa ccaggcgcac tgcgtcggca gcaatcgctg cgcgttcggg
     3661 tgcccgaccg gcgcgaagca gagcgcactc gtcagctacg ttccgcgcgc gctccactac
     3721 ggcgcccgcg tctatgccga cgtgaaggtc gaacgcatca cgcgtcacgg caagcgcgcg
     3781 accggcgtga tcggtcacgt ccgtcatccc gacgggcggc gcggtcacaa cgtcgccgtg
     3841 cgcgcgaagc tcgtcgtcgc cgcgtgcggc gcgatccaca cgcccgcgct gctcgtgcgc
     3901 tcgggtttcc gctcgccgtc gggccgcatc ggccacaacc tgtcgatgca cccgaacgtg
     3961 aaggtcatcg cgatcatgga ttcgcaggtg actggctgga agggcgcgca ccaggcctac
     4021 caggtgcgcg agttccagga tcagggcctc gtgttcgcgt cgatcaactt gccgccgagc
     4081 ctgatggcga tgtcgctgcc gcatcgcggc cgcgcgctcg gcgagctgat gggtcagtac
     4141 gaccacctga tgctcgccgg catgctgtgc gaggacaccg cgaccggccg cgtccgtacg
     4201 atcgatggca agccgcaggc gttctaccag ctcaacgatc acgacgctgc gaatctgcag
     4261 cgcggcgtgt cgctgctctc cgagctgctg ttcgccgccg gcgcgacgcg gatcatgctg
     4321 ccattccacg gcgccgcgga tctgtgctcg cccgacgacg cgcgccgctt gctcgatcag
     4381 aagatcgcgg ccagcggctg ggaggtgctc accgtccaca tgatgggcac cgcggcgatg
     4441 ggtaccgatc ggctgagcgc ggtcaccgat cagttcggtc aggtctacga cgccgatcgc
     4501 ttgatcgtcg ccgatgcttc gttgttcccg acgccgatcg gcgtcaatcc gatggaaacg
     4561 atcatggcgc tagccactcg ctgcgcagcg cacgtgatcg acaacgctgg gaggctcctg
     4621 tcatgactgc tgccgtcgcg aagccacgta tcgtcaccga atcgaagctg gtgggcactg
     4681 cactgccatc gcaggctcac ctcgcgctcg agcaaacgtc gctgtgggac ctcgagaaga
     4741 tcttcgtgcg cggcacgaca ccgaatctcg acgagctcgt cggctgggaa ttcc
//
LOCUS       DASUFX010000225         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1840082, whole genome shotgun sequence.
ACCESSION   DASUFX010000225 DASUFX010000000
VERSION     DASUFX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840082"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="VFV99_08860"
     CDS             complement(<1..2028)
                     /locus_tag="VFV99_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059456.1"
                     /translation="MVKSVPAKPPPIPAAASRPRSLVRIDTEPEPSRTPRSPLVGRSD
                     ELHSLREIVARAIDFQAPQLVSIVGNQGTGKTRLINELITGLREQNNECRVFHGAAER
                     DTTGKLVRFAAISSLLRARFELTPNPDEASKLRFSHEIRTVFSSDQVSEMLHFLGGFV
                     GLDFAPTPFLRAVTENAKQHSELSRTALRRFIELDAANGPAVLVFDDLQWADDDTLAL
                     INDLAVGLGGTPVVLVCAARPEMLVRSAGWGEGAVDHTRIDLRNLDEDDAEAMFKHLL
                     TRCKDIPEDTIQTAVEMTGGNPAFLEQLVRLFMENGAIDASGQVWRLDPDLAAETELP
                     VTIEEAIEARIAALEAEERDLLEKAAVFGNVFWVSAVIALTRLEKPAPPIPTGPLVLE
                     WGNGEDVRRRVSDLIYILADRDYLLPLDADDSSIPGDVEVVFKHNLERELIVRSTEPG
                     KLARYYLSAAQWLESKLSSLRDEQLEFLANLYERGGDRRRAARCYLQGGDRARARYSP
                     DEARNLYEKGLAMLGEADAPARLDALHNLGDVLEQAGRSDDAEQYFNTMLQLAWRYDN
                     LNKAGAAYSRLARAHRRLGKYDSAMEHLRRAHELFERSRDDRGIASTLDDMGRVHWLR
                     GGYSQALDFHRQALTIRRALGDRRSIALSLANIGRVHHDSGNFKAAIAQFRE"
BASE COUNT          328 a          678 c          684 g          356 t
ORIGIN      
        1 ctcgcggaac tgcgcgatcg ccgccttgaa gttgccgcta tcgtgatgca cgcggccgat
       61 gtttgcgagc gacagcgcga tcgaacgccg atcgccgagc gcgcgccgga tcgtcagcgc
      121 ctggcggtgg aagtcgagtg cctgcgagta gccaccgcgc agccagtgca cgcggcccat
      181 gtcgtcgagc gtcgatgcga taccgcggtc atcgcgcgag cgttcgaaca gctcgtgcgc
      241 gcgacgcaga tgctccatcg ccgagtcgta cttgccgagg cggcgatgcg cacgcgcgag
      301 tcggctgtac gccgcgcccg ccttgttgag gttgtcgtag cgccaggcga gctggagcat
      361 cgtgttgaag tactgctccg cgtcgtcgga gcggcccgct tgctcgagca cgtcgccgag
      421 gttgtgcagt gcatcgagcc gcgcaggtgc gtcggcctcg ccgagcatcg cgagcccctt
      481 ctcgtacagg ttgcgcgctt cgtcgggcga ataccgcgca cgcgcacgat cgccgccctg
      541 cagatagcag cgcgccgcac gccggcgatc gccgccgcgc tcgtagaggt tcgcgaggaa
      601 ctcgagctgc tcgtcgcgca gcgacgacaa cttcgactcc agccactgcg cagccgacag
      661 gtagtagcgc gcgagcttgc cgggttccgt cgagcgcacg atcagctcgc gctcgaggtt
      721 gtgcttgaac acgacctcga cgtcgcccgg aatgctcgag tcgtccgcat cgagcggcag
      781 caggtagtcg cgatcggcga ggatatagat gaggtcgctg acccgccggc gcacgtcctc
      841 gccgttgccc cattcgagga cgagcggccc cgtcggaatc ggcggtgccg gcttttccag
      901 ccgcgtcagc gcgatcaccg cggacaccca gaacacgttg ccaaacaccg cggccttctc
      961 gagcaggtcg cgctcctccg cttcgagcgc ggcgatgcgt gcctcgatcg cttcttcgat
     1021 cgtgaccggc agctcggtct ctgccgcgag atctggatcg agacgccaca cctggccgga
     1081 tgcgtcgatc gccccgtttt ccatgaacag ccgcacgagc tgttcgagga acgccgggtt
     1141 gccgccggtc atttcgacgg cggtctggat cgtgtcttcg gggatgtctt tgcagcgcgt
     1201 gagcagatgc ttgaacatcg cctcggcgtc atcctcgtcg aggttacgca gatcgatgcg
     1261 cgtgtgatcg accgcgcctt cgccccagcc cgcgctgcgc acgagcatct cgggacgcgc
     1321 cgcgcacacc agcacgaccg gcgtgccacc gaggccaacc gcgagatcgt tgatcagcgc
     1381 gagcgtgtcg tcgtcggccc actgcagatc gtcgaacacg aggacggccg gaccgttcgc
     1441 ggcgtcgagc tcgatgaacc ggcgcagcgc cgtgcgagac agctcgctgt gttgcttcgc
     1501 gttctcggtg actgcccgca agaacggggt cggcgcgaag tcgaggccga cgaagccacc
     1561 gaggaagtgc agcatctcgg agacctgatc cgacgagaac acggtgcgga tctcgtgcga
     1621 gaagcgaagc ttgctggcct cgtcgggatt cggtgtcagc tcgaaccgcg cgcgaagcag
     1681 cgacgaaatc gccgcgaagc gcacgagctt gccggtcgta tcgcgctcgg cggcgccgtg
     1741 aaacacgcgg cactcgttgt tctgctcgcg cagtcccgtg atcagctcgt tgatcaggcg
     1801 cgtcttgccc gtgccttggt tgccgacgat cgacacgagt tgcggcgctt gaaaatcaat
     1861 cgcacgcgca acgatctcgc gcagcgagtg cagctcgtca ctacgaccta ccagcggact
     1921 gcgcggcgtg cgcgacggtt ctggttcggt atcgatgcgt accaagctgc gcggacgtga
     1981 tgcagctgcc gggatcggcg gtggttttgc ggggacgctt ttgaccacac ccatcccttt
     2041 gtatgg
//
LOCUS       DASUFX010000226         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1701617, whole genome shotgun sequence.
ACCESSION   DASUFX010000226 DASUFX010000000
VERSION     DASUFX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701617"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..427
                     /locus_tag="VFV99_08865"
     CDS             29..427
                     /locus_tag="VFV99_08865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5059457.1"
                     /translation="MSERFSFPITADEADIDGLGHVSNLVYLRWVLEAATAHSTARGW
                     DLDAYKQLGSVWVVRRHELDYLGQVTVGQSLVAETWVDSWKQVSCVRKTELVRDGQVV
                     ARCATTWAFLSLASGRPQRIPEQLLAAFQR"
     gene            679..2250
                     /locus_tag="VFV99_08870"
     CDS             679..2250
                     /locus_tag="VFV99_08870"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5059458.1"
                     /translation="MKGNRALFAAMAALCLIPSCTSDSPDEVDSPVDSPSLDDKFAEA
                     ARIYDVPVDLLKAIAYVETGWQNVAGEDDELGRVAGAGVFGLWGDNLERGAAAAQLAV
                     DDVRTDYDANILAAAARLSELAAQHGISGSDLMAWQPVIADFAQNPDDEARAEYVNGV
                     ISVLSGGASSVAEDGTTIATISPHVEIGMPEITTLAAGGDYANAIFRSSPNYNSRNGY
                     HVSLVVIHSCEGNYAGCWGWLRNTAAQASAHYVVSENGGEVTQLVREANRAWHVAASY
                     NCHYAGDQQCNLQGVSTNNFSVGIEHAGFASQASWSNGIIETSARLTCDITKRQGVIR
                     DRNHIVSHGQLQPYNRTDPGPHWPWSHYIDRVRQICGDGGGGGGGGGGGAGIIIDSNN
                     ANNNQQVAKIELAGAWTASSSTAGYYGSGYYFANTSATSAPATFWFYLPAAGSKTIDA
                     WWTAGTNRASAAPWIAYNAAGTEVGRASTNQRTNGSKWVALGTWNFSAGWNKVVLSRW
                     TTSGDVVIADAVRVR"
     gene            2247..3179
                     /locus_tag="VFV99_08875"
     CDS             2247..3179
                     /locus_tag="VFV99_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX5059459.1"
                     /translation="MKRLVVPVLVVLAACGGDDKPARSELPGTIYFVEDVPHAQLVKL
                     AGGTRTVIGRDLYPSPSTLFDGRIVAIASRGDGSANSEQLALIGSHVERIGPAAAQLR
                     DPAVDSRGRIVVAANFDGASNLYRIDLDGGITQLTNDPQGNFAPATIGESVVYVSSRD
                     GDAEIYRDQQRLTAFHRDDWQPTPSPDGKTIAFLSDRDGTPHIYVMSSDGTNLRRLTM
                     HDGEETSPTWSRDGKQLAYTVDNQIWLRDLKTGNDRQLTPPGTNDVEPSFSPDGHWLS
                     VSRARGDASDVWVISLDSGEGQPVAQNARLPRWF"
     gene            3357..3764
                     /locus_tag="VFV99_08880"
     CDS             3357..3764
                     /locus_tag="VFV99_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059460.1"
                     /translation="MRAPDAGTMSGGGTFMCRTKVGSVGSGNHNAGMDCQGVCHNHGF
                     TLSGTVFTSAGGGVAVVGASITVTDKGGKTFDMVTQQNGNFYTTTPVQFPVTVVASSC
                     PDVQKMPVAITSAGGCNRNGCHQIGAEGHIHLP"
     gene            3901..4386
                     /locus_tag="VFV99_08885"
     CDS             3901..4386
                     /locus_tag="VFV99_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059461.1"
                     /translation="MHLLLGFVVLAGCIGSIDGIEDNTSTGDGGTHTNTPDAPKPPCK
                     NKVNAANDGHHYPGMDCQEGCHNHGFSLSGTLFTGVTGTTPIVGATITVKDASGATFE
                     MISQTNGNFYSANAMQFPVTVVASSCPDAHAMVAPITAPGGCAAAGCHVAGATGNVFL
                     P"
BASE COUNT          784 a         1431 c         1465 g          746 t
ORIGIN      
        1 gggacgttaa caggtacatt gcgccgccgt gtccgagcgc ttcagctttc cgatcaccgc
       61 cgacgaggcc gacatcgacg gtctcggtca cgtctcgaac ctcgtctact tgcgctgggt
      121 tctcgaagcc gcaaccgcgc actcgaccgc ccgcggctgg gacctcgacg cttacaagca
      181 gctgggcagc gtgtgggtcg tgcgccgcca cgagctcgac tatctgggcc aggtcacggt
      241 cggccagtca cttgtcgccg agacgtgggt cgactcgtgg aagcaggtct cgtgcgtgcg
      301 caagaccgag ctcgtccgcg atggtcaggt cgtcgcgcgg tgcgccacga catgggcgtt
      361 cctctcgctc gcgagcggcc ggccgcagcg aattcccgag cagctcctcg cggcatttca
      421 gcgctgactg gctgacacaa acagtgaagt cgatgcgctg actgacacca ctgactgaca
      481 tcgcgcgaga cttcagcgtc tcgacggcgc gtgacacacg ctcaggttgc accgacggga
      541 cgaaacactg caaacgcacg tgcgcagcgt cgccgaatgc cgtgcgcgct tgtgcgcaaa
      601 tcgcatctgg agctgcgcat tcgcatgacg atgtgtgcag acgtagttgg catttcgcgt
      661 gaactaggcg cgggtcgtat gaagggaaac cgcgcgttgt tcgcggcaat ggccgcgcta
      721 tgtctaattc cgtcgtgcac gagcgattcg cccgacgaag tcgattcgcc tgtcgattcg
      781 ccttcgctcg acgacaagtt cgccgaagcg gcacgcatct atgatgtgcc ggtcgatctg
      841 ctgaaagcga tcgcgtacgt cgagaccggc tggcagaacg tcgccggcga agacgacgag
      901 ctcggtcgcg tcgccggtgc tggcgtgttc ggcttgtggg gcgacaacct cgaacgtggc
      961 gcagctgcag cgcagcttgc ggtcgatgac gtgaggaccg actacgacgc gaacatcctg
     1021 gcagcggccg cgcggctcag cgagcttgcg gcgcagcacg gcatcagtgg cagcgacctg
     1081 atggcgtggc agccggtgat cgccgacttc gcacagaacc ccgatgacga agcgcgtgcc
     1141 gagtacgtca acggcgtgat cagcgtgctc tcgggtggcg catcgtccgt cgcggaggac
     1201 ggcacgacga tcgcgacgat cagcccgcac gtcgagatcg gcatgccgga gatcacgacg
     1261 ctcgcggccg gcggtgacta cgcgaacgcg atcttccgct cgtcgccgaa ctacaactct
     1321 cgcaacggct accacgtgtc gctcgtcgtg attcacagct gcgaaggcaa ctacgccggc
     1381 tgctggggct ggctgcgcaa caccgccgcg caggcgagcg cgcactatgt cgtgagcgag
     1441 aacggcggcg aggtcacgca gctcgtccgc gaagcgaatc gcgcatggca cgtcgcagcg
     1501 tcgtacaact gccactacgc aggcgaccag cagtgcaacc tgcaaggcgt gtcgacgaac
     1561 aacttctcgg tcggcatcga gcacgccggc ttcgcatcgc aggcgagctg gtcgaacggc
     1621 atcatcgaga cgtcggcgcg actgacgtgt gacatcacga agcgccaggg cgtgattcgc
     1681 gatcgcaacc acatcgtgtc gcacggccag ctgcagccgt acaaccgcac cgatccgggt
     1741 ccgcactggc cgtggagcca ctatatcgat cgcgtacgcc agatctgcgg cgacggcggc
     1801 ggtggcggcg gcggcggcgg tgggggagcc ggcatcatca tcgacagcaa caacgcgaac
     1861 aacaaccagc aggttgcgaa gatcgagctc gctggtgcgt ggaccgcatc gtcgtcgacg
     1921 gctggctact acggcagcgg ctactacttc gcgaacacgt cggcgacgtc ggcgcccgcg
     1981 acgttctggt tctacctgcc cgccgccggc tcgaagacga tcgacgcgtg gtggaccgcg
     2041 ggcacgaacc gcgcgagcgc tgcgccgtgg atcgcgtaca acgcggcggg caccgaggtc
     2101 ggtcgtgcaa gcacgaacca gcgcacgaac ggcagcaagt gggtcgcgct cggcacgtgg
     2161 aacttctctg cgggctggaa caaggtcgtg ctgtcgcggt ggacgacgtc gggcgacgtc
     2221 gtgatcgcag acgccgtgcg cgtgcgatga aacgactggt cgtccccgtg ctcgtcgtgc
     2281 tcgccgcatg cggcggtgac gacaagcccg cacgcagcga gctgccgggg acgatctatt
     2341 tcgtcgagga cgtgccgcac gcgcaactcg tgaagcttgc cggcggcacg cgcaccgtga
     2401 tcggtcgcga cttgtatccg tcgccgtcga cgctgttcga tggccgcatc gtcgcgatcg
     2461 cctcccgcgg cgatggctcg gcgaattccg agcagctggc gctgattggt agccacgtcg
     2521 agcgcatcgg cccagcggcg gcgcagctgc gcgatccggc ggtcgatagc cgtggccgga
     2581 tcgtggttgc cgcgaacttc gacggcgcga gcaacctcta ccgcatcgat ctcgacggcg
     2641 gcatcacgca gctcacgaac gatccgcagg gtaatttcgc gcccgccacg atcggcgagt
     2701 cggtcgtcta cgtgtcgagc cgcgacggcg acgccgagat ctatcgcgac cagcagcggc
     2761 tcaccgcatt tcatcgcgac gactggcagc cgaccccatc tcccgacggc aagacgatcg
     2821 cgttcctcag cgaccgcgac ggcacgccgc atatctatgt gatgagctcc gacggcacga
     2881 acctacgccg gctcacgatg catgatggcg aagaaacgtc gccgacgtgg tcgcgtgatg
     2941 gcaagcagct cgcgtacacc gtcgacaatc agatctggct gcgcgacttg aagaccggca
     3001 acgaccgtca gctgacacca ccaggcacca acgatgtcga gccgagtttt tcgcccgacg
     3061 gacattggct gtcagtctcg cgtgcgcgcg gcgatgcgag cgacgtctgg gtgatcagtc
     3121 tcgatagcgg ggaggggcaa cccgttgcac agaacgcgcg cctcccacgc tggttttagt
     3181 cattgcgact ggtttgagct ctgcgtgtat cggcgctgaa caacgtcgac gcggtcttcg
     3241 agttgcaacg cacgcgctgc atgcgaggga tggttttgct tcttcttgtg gcgtgcgatt
     3301 acaccagcgg cgatggcacc tcggggtcca gcgctagtga ggagccggat gcgggcatgc
     3361 gtgcgcccga tgcgggcacg atgagtggcg gtggcacatt catgtgccgc acgaaggtcg
     3421 gcagcgtcgg cagtggaaat cacaacgccg gcatggactg ccaaggcgtc tgtcacaacc
     3481 atggcttcac gttgtcgggc accgtgttca ccagcgcggg cggtggcgtt gcggtcgtcg
     3541 gcgcgtcgat caccgtgacg gacaagggcg gcaagacgtt cgacatggtc acgcagcaga
     3601 acggcaactt ctacacgacg acgccggtgc agtttcccgt caccgtggtc gcgagctcgt
     3661 gtccggacgt gcagaagatg cccgtcgcga tcacgagcgc cggtggttgc aaccgaaatg
     3721 ggtgccacca gatcggagcc gagggccaca tccatctgcc gtagccgcgg gtcaccagat
     3781 cggcgccttg ggtcacagct tgtttcggcg ccgatcgcct cggaaatctg agggttccag
     3841 tgttttgcgg cactttcagc cctgcggcgc ttcggccctg cagttgcaac ctgggccccc
     3901 atgcatttgt tgctggggtt cgttgtcctc gctggttgta tcggctcgat cgatgggatc
     3961 gaagacaaca cctccacggg cgacggcggc actcacacga acacgcccga tgcgccgaag
     4021 ccgccgtgca agaacaaggt gaacgccgcc aatgacggcc accactatcc cggcatggac
     4081 tgccaggagg gttgccacaa ccacggcttc tcgctctccg gcacgctgtt caccggcgtc
     4141 accggcacga cgccgatcgt cggcgcgaca atcaccgtga aggacgcctc gggcgcgacg
     4201 ttcgagatga tctcgcagac gaacggcaac ttctattcgg ccaacgcgat gcagttcccg
     4261 gtcaccgtcg tcgcgagctc gtgtcccgat gcgcatgcga tggtcgcgcc gatcaccgcg
     4321 cccggtggct gcgccgccgc gggctgtcac gtcgccggcg cgaccggcaa cgtgttcttg
     4381 ccttagtgca gcttttcgat cgcgcgcgcg atcgcggcgg cgagct
//
LOCUS       DASUFX010000227        19231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1145673, whole genome shotgun sequence.
ACCESSION   DASUFX010000227 DASUFX010000000
VERSION     DASUFX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145673"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFV99_08890"
     CDS             complement(<1..437)
                     /locus_tag="VFV99_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059462.1"
                     /translation="MWTLARKLLLHDRLRFAVAIAGVSVSVMLVLVQVGLYFGFMNTA
                     SSIIDASKADLWVGKAGNESFEFASPFDERTLYKVASVPGVERTERVLMNFAQFKLAD
                     GGDLGVQIVGVETGPGHTPLLAPWRVVAGDVNRLGEAGAIVVD"
     gene            complement(540..899)
                     /locus_tag="VFV99_08895"
     CDS             complement(540..899)
                     /locus_tag="VFV99_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059463.1"
                     /translation="MPASILVVDDNRDAADLMGEALRMVGYTVRVAHEPTEALAMLDD
                     FTPDAALLDIGLPGIDGYTLAGELHRRLPALPLIAITGYGQANDRARAVAAGFAAHFV
                     KPASIHDLVAVLERLLA"
     gene            complement(992..2887)
                     /locus_tag="VFV99_08900"
     CDS             complement(992..2887)
                     /locus_tag="VFV99_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059464.1"
                     /translation="MIGGLLTIVIAAVGAAYAALVIRAAPTRRDNLMFGVLALTDASM
                     ILWRGVNVLSGESIVSASVTLPCSIGTIVMALITMDFLSGFPRRPAMRWRWRLALIAW
                     GIVGFASIAFVDAGKHWDAFRLAELTFFLPSTAVVFVLGAIAWRRTSDRDARTVIAML
                     WFRWGFGLVAYTLGPLLGVFEEMVWAETTVATLLSFVIIGTAVLRSDLFSIRSAAAEA
                     VLATTMAFGVVLSGGGAIFAVLRYTAPGALQTALLIGATLVPLHVAVIFRALYPRLEK
                     NVLAGLDERRARRLGMQGEPIPVEPTLAIDEACKRISSIGDGATVRWQTAAQLPPALA
                     TALKSGEPQRKDETPDIPACFVVPALGADRSLVGAFMIEHGTIDRDTYVVARDLAARI
                     ALVVERQQAISELEDARRLAALGSFAAAIAHDIRTPLTSISLNVQILRRKLQLSDDDR
                     EHLDIALEELARLDKSVAEILDFAKPVKLVPQAIDVGELIETTTRGLSPVLSEKGVSL
                     HCEPQGELPTVHGDPQRLRQVLVNLVGNAADASTPGTRVTVRASIYSPAADAAHVAIE
                     VEDKGRGIKAEDLPRIFEPFFTTRPDGTGLGLAICHKVVRAHGGDIQVRSAVGEGSTF
                     TILLPAA"
     gene            complement(2884..4347)
                     /locus_tag="VFV99_08905"
     CDS             complement(2884..4347)
                     /locus_tag="VFV99_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5059465.1"
                     /translation="MARILIVDDDRSIRRTLEKFLGGEGYEVATAQDAPGAIAAVNSN
                     VAASGTASSATHGATSASSASGATSASNASGAPIDLMLLDLGLPGGSGFDVLNALVDR
                     DDAPTVVVVTARDDMQSTVKAIQLGAYEYLVKPVDIDRLGSVVKSALASREARGELVE
                     LASRGPEVGDILGKSPAIRDVWKQIGAVSTTRAPVLIRGESGTGKELVARAIHNAAEL
                     GMRGARGNERPFIAVNCTALAPGVLESELFGHVKGAFTGAVADRPGRFELAGKGTIFL
                     DEIAEIPLDLQGKLLRVLQERTFERVGDAKPMPLDARVIAATHRDLAQMVKQGTFRED
                     LYYRLRVVEIPLPPLRDRASDIPVLVEGLLAKINRDLDKDVRYVTPAALAKLTSYAWP
                     GNVRELENTLTRAVVLAKSDVLDETLLPLGTAEAPQADTDSEGSLPTLREIERRHIAR
                     VLAHTEWNKRRACAILDISRPTLDRKIDEYGLRKDET"
     gene            4526..5257
                     /locus_tag="VFV99_08910"
     CDS             4526..5257
                     /locus_tag="VFV99_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5059466.1"
                     /translation="MSETWTLSELVDEAAQRLEALPPPKNGQVRAVPDERTIRYYGTI
                     GLLDRPAAMRGRTALYSRRHLAQVVAIKRMQAAGHSLADIQAMWSTLDDTTLGRMSGV
                     QVATGKAKAARKEFWKREATMTELPPPSVPMPQAPTGPTIPMPMPMPTPTPMTPMPAS
                     NAPQMLPLPTFTPYSAAGAGTATTINLPLEGGVSIVITVDEDAAVSLSPADVRALRAA
                     AAPLVTELAKRGFIGSSKATLEEGP"
     gene            5263..7788
                     /locus_tag="VFV99_08915"
     CDS             5263..7788
                     /locus_tag="VFV99_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5059467.1"
                     /translation="MPIVLMTEEEVAGYGRSSDAGFGALKTERGLLPLAAMDVDARIA
                     GVVASIEIAQTFVNTTGAAIEATYIFPLPDRAAVHRFRMEVNGRVIEGVIDERGAARE
                     QYDEAIAAGHRAAITEQERPGVFTLRVGNLMPRESATVRLSLVGPLPVDDGEVTFQFP
                     LVVAPRYMPGAGLGGDQAGLGIAEDTSLVPDASRISPPVLLPGCPNPVRLGMRVSLAD
                     HHVRDVSSSLHAVTTTLRDAQVIEIQPGEKLNRDFILRWHIDGKELSSSLVCSDDADG
                     KGGTFALTLVPPSTLAVSQKPRDVVFVIDRSGSMSGWKMLAARRATARMIDTLTSRDR
                     FMVIAFDDNIEVQPESKLAHASDRTRFTALEVLAKIDAEGGTELAQPLQLAVSTLAGG
                     HDDRERVIVLVTDGQVGNEDHILREIAPSLKNVRVFTLGIDKAVNAGFLRRFAGAGGG
                     LCELVESEDRLDAVMAKVHRRIGTPVATELRLEPRGITVDASTIAPAKLPDVYAGAPV
                     VVFGRYKGAAASDASLAVSGTTYGDPMKQTVARDAQSPGAWLAASWARAHIRELEDKY
                     VLGARELEGEIVRVSKTFSVLSRFTAFLAVDRSEVANKGGNVVQVVQPVEEPAGWDMK
                     VGGFGGQTRRRLPLGKARGGRPGAAMPPPMASMPSMSAPMAKKTSMPIGGMSAGPARF
                     GPMDDMKEITRGIAPSGPNASPPRPPMAPPLPAPPPPQAQYAPMVHDKAREKAVAGDA
                     SAYLAKLAQLARGLTTHAIAGDVGALRLLRQRLTEWVEDLRSVGGHDALADAVEHEVK
                     RLSVALAGTGDLATEARAIATELSRLASGATPPKKSGRAEFWK"
     gene            complement(7880..8353)
                     /locus_tag="VFV99_08920"
     CDS             complement(7880..8353)
                     /locus_tag="VFV99_08920"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5059468.1"
                     /translation="MRIQRIHVLAAISERRLDILMRQLPKEQRMGRPWSRPLRHRLLL
                     ACVALRTDLTLRELAAILQISKSSAHRILATITPRLAALAVDVKADRRWSWIVDGTLI
                     PTRDHAGAAKSKNYRWSCNAQLLVPRRDLRVVAISGGGPGNRNDPIHYRGSTVAA"
     gene            8382..10367
                     /locus_tag="VFV99_08925"
     CDS             8382..10367
                     /locus_tag="VFV99_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059469.1"
                     /translation="MASSGKRTRGANKQTPWSRNPDDGVSVLRVGLDTSDPRQRARVE
                     EIFSAAYSIKRAVQRDARDRCRAYWQATHERERGPSAVRDRLGLSRKALENAAYAHLN
                     GAPHLRRFATKALAMHLADSVWTATERHLFGDARGRRAGAPRIGRWYDFQWLPGRARS
                     HRRKRKWETFRLHGTLAGHRAAYTHRDGDFVQPRRLRPVELTTKTCWDYTGPLAVVFS
                     GLADGTLVLPVRLPTAPSNQPMLDHHLADPSKWHKVDLVRHRDPNAEGGWRYEAHLMV
                     LTTPYVSPATAVRRAKVAIESVDRTAGIDVNVSCLAIASHDTGKAMRLTRVERSGEDK
                     QRDRGRRRRERRRQRELERSRRAANRAQYQLSKRQEKRARRRAARGLPPVDEIPMGPR
                     KARADGVPLQRYQRDQLSARFRRTRAAQAAEAEAGARARRDHAREVAAGVVATHGYQL
                     VVEDCTIAAWASSWGTAVAAFTPAMLVAAIDREARAVAAIAGGEGGVARATTRTTALS
                     QHCPCGARVQKRLVDRVHRCPDCGLVGDRDDVAAVLASFVVQIQLGEPTSARVDYAAA
                     SDALDEIRRVFGSSYQGWQDTLSESNDLSARDGSFIAWTTSTSDPVAMARRNVGTASC
                     STLNEPGSRQTTSERARVRTDRSTKYVPRWTYLRDTS"
     gene            10396..11139
                     /locus_tag="VFV99_08930"
     CDS             10396..11139
                     /locus_tag="VFV99_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX5059470.1"
                     /translation="MRWCVVVFVAVFAVSACSKPAPHRDDVHVVAPPAADATPVAVVA
                     DAAVDAPSVAIVDAPMVWSAEREQLTLDYRRAHSDANAKDLAIDPKVIVLHYTGGNSA
                     KATRTYFDNVKIEAARKELARGGAVNVSSHFLVDRDGTIYRLQPETRFARHCIGLNHV
                     AIGIENVGDDKHPLTDAQVAADAALVRELAHRFHITHLLGHYEVMRFRDHPYYVELDR
                     AYKNEKDDPGAAFMAHVRERVADLGLAGL"
     gene            complement(11143..14106)
                     /locus_tag="VFV99_08935"
     CDS             complement(11143..14106)
                     /locus_tag="VFV99_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059471.1"
                     /translation="MTSGPPLYLVSACGSAEEFVAAFRRYADRTGLFVPSAAPLPAGR
                     RGRLALTLKDGGVMIEGEAEILQSSAKPTVLHGRPGMTVKFIEPDEPSKVVIGELEKA
                     RLAMKPAPPSVPPRPADIPAQPRPVVPAPSGRIDAANALAECVVIGDPATLIATANAP
                     RPAGDSKFAVPSIPAVGTPRPKTPSAPPEMRPKTPSTPPEMRPKTPTAPPDPTATPLP
                     APKVANNSGKLTSIGFPAIDKMPAKTESGRVVSNATTLGMPALDRKPDPMLSPQRGTP
                     AAANPTSPPLAAPSSAPSASAPVTDGKPARPDATPPKFIPNTTVPGTGAPDNAATKPL
                     TVHDEQTTIGEGPPKRQTDPIPATIPAKPLFDPIGEDEATAIGAVPPKKVTDPMPTTA
                     PPTTAPPTPTMQATPVAAAREPSAPTKVETPRSELSKQHKATSIGFPVVGRNVFETQP
                     LGIVPPANPAKYAAETEPQPPTLPKSTKAQQPAAPRGKAPTTPPLTPRHPTPVAPVPI
                     VRMPSKAAPSAAPEDEPTDIGNLPTVTAPVDVSSPSDSGAIVQTPPARSGGMRASEIL
                     AAIPAGDWTMSPDESVPHALPPEAKVAAVAPPSEPTPAPAPPPKGPPTGDWTIQLDPA
                     GGGWSEPEKVDKAESATAGNPVLAVASEKPLDVVQWDEKPTGIGESKIEIDSTLMEAK
                     ALPDDEPAQIVSTKPQDLPPPLEPMPPRRTGVFADPGAYAQQPYRPSATDIVTYPAAP
                     QDAAKKKKMLLLAIAAGLVAVGAIVMLVLTLGGAKKKAASTTGTGAASGKMTVTPPPA
                     VDAPAAVTPPQGSGSQQVAVAPAGSGSETAAPAQGSGSAKPEPTPPVASGPCKVDVKS
                     LPTGADIYVDSKKLGTTPDTIDLPCGTESKLSLKKKSFPNTLRAFTPTAGKANKLLIK
                     LTRTLVSVKVTSTPAGATITAGGKSMGVTPTTIKLPQGEPTAITLSKPGYAKDTQKVS
                     PRSGASHHVTLKKGK"
     gene            complement(14204..14428)
                     /locus_tag="VFV99_08940"
     CDS             complement(14204..14428)
                     /locus_tag="VFV99_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059472.1"
                     /translation="MAKAKKGKSGGGGPKEMLLVASKVKGMIKDAGCNTAGDALDGLN
                     ALVGWYIAQAAKRASENGRKTVRAHDFAIM"
     gene            complement(14535..16064)
                     /locus_tag="VFV99_08945"
     CDS             complement(14535..16064)
                     /locus_tag="VFV99_08945"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX5059473.1"
                     /translation="MRPSSIAVVVACAALPVACSKGEKKAKVETKTETTVNVAPKKEV
                     PNKALPALAADPGGATGKPVWQAGFGGLGIDSPRGIAVGADGSVYICGYIEGETDFGG
                     SIGKKKSAGKSDAFVAKFGPDGKIAWAQTWGATRDDAANAIAVKGDKVVVTGTFLDQI
                     TVGSFTKKSAGSDDLFIAAFDKDGNADWLWTGGGIDSDGVNTIAAMPDGGWVIGGSFT
                     DSLDITTGAKSVSMKSKGRTDAILIKLAASGDTEWVKQFGGNYDDSIMHVAVDANSNI
                     YVQGLFKDIANWGGSDLKAGGGSDNDIVLAKYDVNGDHVWSQRFGNAFNEVAGGVAVD
                     PAGNVTIAGSFENKGGISFGDKDDHQSLGEADVYVARFSGDGKLLWAKTFGGERIDVG
                     YDVALDSVGNAIVTGWFEGTVDFGKGGTISTKNLNKDVFIVKLNAKGETVWVTTFGDK
                     DHDQGRAIAVDDKGSVYVTGLYRFKLALLDPPVEQKIDLNDPVLSKAPKPDVFVVKLD
                     R"
     gene            16114..18327
                     /locus_tag="VFV99_08950"
     CDS             16114..18327
                     /locus_tag="VFV99_08950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5059474.1"
                     /translation="MLRYAIFVATALLVGCPSKQPEAPHSPEDTALRIRIAQAEAHRA
                     AGVDELVQLATSGDVHQRELALRGLGRVGGDKARATLEASLADKDPKVVAAACYAIGV
                     AAALDEPPDWKPGGKLVAVLAVAPLPALEALGRAGDATNQPALTAKLADKDPAIAAAA
                     AIALGRHGRRKIAWSAEARAALATATSHADSNVRYAATYALSREHEPPGDDAVNAALI
                     ARIADEYPETRASAIAGLAKRKAVTAARKQIVESLRDRDWRVAVEAVRALAGANGDDE
                     GRVAVIGSLSVRAAQLEKGSATEAQVIVEALRTLLDHPISNPKSIATELHIVSEPANA
                     SIPALTTGWSECLQQAVQASATSGAPTGTTRGAPTGTASGAPTGTKSGAPTGTTSGAP
                     TGTTSAGAIEALTTCGHGALPDHLRLPLLADVAKGGDIAAKRAALRVLLAHDDPRVRA
                     AGLNLLPETWKDSDAKGQATIVGTISSALAVKNPIVAGTAVDVAGTVYEQLPKDDALR
                     TTLDNAILARAKTETDVELSASLYTAIGKNAIPGGADVCRGGLAAAPARAKAAADCLK
                     ALGEAAPPQAAASAELPPVDVATVIGKKLRWHLVTSRGEIVIELRPDVAPWAVASIVA
                     LTDRGFYNGLEFHRVVGNFVVQGGDPTQSGWGGPGYTLPAEPASTFDGPGYVVGGVGI
                     ADAGKDSGGSQWFIMHSRAPHLDGRYTWIGSVVDGQKYADALLIGDRVERASVER"
     gene            18419..>19231
                     /locus_tag="VFV99_08955"
     CDS             18419..>19231
                     /locus_tag="VFV99_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5059475.1"
                     /translation="MRPLFAAVLPLGIATAAFAEPALAKRMISDFGAPSFQLLGRPIG
                     GAKPARETPAYLAGQRIAAVGDGALVIDSDSGDLIKTDKAGTRIASIAIGKNAGLMVY
                     DPTAKLAYVVDRLASKVKVVKVGERLEIAATHATPAEPYGIALSPDRKTVMVTTIADR
                     TLVALDTANGKEKWRTALGREPRGIAVSPDGTRALVAYLTTGTVDQVDLLESHKAEHI
                     ALSTQNMRRGRFGGGDAFARGAFTALFLGDQEAVVPFQRETPVQAIGGGERTG"
BASE COUNT         3053 a         6353 c         6668 g         3157 t
ORIGIN      
        1 ttgtcgacga cgatcgcgcc ggcttcgccg agccgattca cgtcgcccgc gacgacgcgc
       61 cacggtgcga gcagcggcgt gtgcccgggc cctgtctcga cgccgacgat ctgcacgccg
      121 aggtcgccgc catcggcgag cttgaattgc gcgaagttca tcagcacgcg ctcggtgcgc
      181 tcgacgccgg ggaccgacgc gaccttgtag agcgtgcgct cgtcgaatgg cgacgcgaac
      241 tcgaatgact cgttgcccgc cttgccgacc cagaggtccg ctttgctcgc gtcgatgatc
      301 gacgacgcgg tgttcatgaa gccgaagtag agaccgacct ggacgagcac cagcatcacc
      361 gacaccgaga cgcccgcgat cgcaacggcg aagcgcagcc ggtcgtgaag caagagcttg
      421 cgagcgaggg tccacatgcc gagcacattg agcaggcgct gtgccggcgt cttgcccttg
      481 tgaatcgcga ggttgcgcgg cgggcggcgg taacgagtcg ttaccctggc gacggagcgt
      541 tacgccagca ggcgctcgag caccgccacg agatcgtgaa tcgaggccgg cttgacgaag
      601 tgcgcggcga agcctgctgc gaccgcgcga gcgcgatcgt tcgcttggcc gtagccggtg
      661 atcgcgatca atggcagcgc aggaagccgg cgatgcaact cgcctgcgag cgtgtagcca
      721 tcgatcccgg gcaggccgat gtcgagcagc gcggcgtctg gcgtgaagtc atcgagcatc
      781 gcgagtgctt cagtcggctc gtgtgcgacg cgaacggtgt agccgaccat gcgcagcgct
      841 tcgcccatga gatcggccgc gtcgcggttg tcgtcgacga ccaaaatcga cgccggcatg
      901 acgcgggcag actgcgtaag cgcacgcgat ctgtccagtg gaactcgatc tcacgtacgc
      961 gagcaccgtg cgccctcgcc acagcgcacg gtcatgcggc cgggagcagg atcgtgaacg
     1021 tcgatccttc accgactgca gagcgcacct ggatgtcgcc gccgtgcgca cggacgacct
     1081 tgtgacagat cgcgagcccg agcccggtgc catcgggacg cgtcgtgaag aacggctcga
     1141 agatgcgcgg caggtcctcg gccttgatgc cgcgaccctt gtcttcgacc tcgatcgcca
     1201 cgtgcgctgc gtcggcagcc ggagagtaga tcgatgcgcg cacggtgact cgagtgccgg
     1261 gagtgctggc atcggcggcg ttgccgacga gattgacgag gacctggcga aggcgctgcg
     1321 gatcgccgtg cacggtcggc agctcgcctt gtggttcgca gtgcagcgag acgccctttt
     1381 cagagaggac aggcgagagg ccacgcgtcg tggtttcgat caactcgccg acgtcgatcg
     1441 cctgcgggac gagcttcacg ggcttcgcga agtcgagaat ctcggcgacc gacttgtcga
     1501 ggcgggcgag ctcttcgagt gcgatgtcga ggtgctcgcg atcgtcgtcg gacagctgga
     1561 gcttgcggcg caagatctgg acgttgagcg agatgctggt gagcggcgtg cggatgtcgt
     1621 gcgcgatcgc ggcggcgaac gaaccgagcg cggcgagccg gcgtgcatcc tcgagctcgc
     1681 tgatcgcttg ctggcgctcg acgacgagcg cgatgcgggc ggcgaggtcg cgcgcgacga
     1741 cgtacgtgtc gcgatcgatc gtgccgtgct cgatcatgaa cgcgccgacg agcgagcgat
     1801 cggcgccgag cgccgggacg acgaagcacg cggggatgtc gggcgtctcg tctttgcgtt
     1861 gcggctcgcc gctcttcagc gcggtcgcga gggcaggggg aagttgcgcg gcggtctgcc
     1921 agcgcacggt cgcgccgtcg ccgatcgacg agatgcgctt gcacgcttca tcgatcgcga
     1981 gcgtgggttc gacgggaatc ggctcgcctt gcatgccgag ccggcgggcg cggcgctcgt
     2041 cgaggccggc gagcacgttc ttctcgaggc gcggatagag cgcgcggaag atcacggcga
     2101 cgtggagcgg cacgagcgtg gcgccgatca gcagcgcggt ctgcagcgcg ccgggggcgg
     2161 tgtagcggag caccgcgaag atcgcaccgc cgccggacaa gacgacgccg aatgccattg
     2221 tcgtcgcgag cacggcttcg gcggcggccg agcgaatcga gaacagatcc gagcgcagca
     2281 ccgcggtgcc gatgatcacg aagctgagca gcgtcgcgac ggtggtctcg gcccatacca
     2341 tctcctcgaa gacaccgagc agcgggccaa gcgtatacgc gaccaagccg aagccccagc
     2401 ggaaccacag catggcgatc acggtcctgg cgtcgcggtc gctggtccga cgccacgcga
     2461 tcgcgccgag cacgaacacg accgccgtcg acggcaggaa gaacgtgagc tcggcgagcc
     2521 ggaacgcgtc ccaatgcttg ccggcgtcga cgaacgcaat gctcgcgaag ccgacgatgc
     2581 cccacgcgat cagggcgagg cgccagcgcc agcgcatcgc cggccggcgt gggaagccgg
     2641 acaagaagtc catcgtgatg agcgccatca cgatggtgcc gatcgagcac ggcagcgtca
     2701 ccgacgcgct gacgatcgac tcgcccgaga gcacgttgac accgcgccac aagatcatcg
     2761 aggcatcggt cagcgcgagc acgccgaaca tgaggttgtc gcggcgggtc ggtgccgcgc
     2821 ggatcacgag cgcggcgtag gcggcgccga cggcggcgat gacgatcgtg agcaggccgc
     2881 cgatcacgtc tcgtctttcc gaagaccgta ttcatcgatc ttccggtcga gcgtcgggcg
     2941 cgagatgtcg aggatcgcgc acgcgcggcg cttgttccat tccgtgtgcg cgagcacgcg
     3001 tgcgatgtgg cgacgctcga tctcgcgcag cgtcggcagc gagccttcgc tgtcggtgtc
     3061 ggcctgcggt gcctcggcgg tgccgagcgg cagcagcgtt tcgtcgagga cgtcggactt
     3121 cgcgagcacg accgcgcggg taagcgtgtt ctcgagctcg cgcacgttgc cgggccacgc
     3181 gtagctggtc agcttcgcga gcgcggcggg cgtgacatag cgaacgtcct tgtcgaggtc
     3241 gcggttgatc ttggcgagca agccttcgac gaggaccggg atgtcgctcg cgcgatcgcg
     3301 cagcggcggc agcggaatct cgacgacgcg cagccggtag tagaggtcct cgcgaaacgt
     3361 gccctgcttc accatctgcg cgaggtcgcg atgggttgcc gcgatcacgc gcgcgtcgag
     3421 tggcatcggc ttcgcgtcgc cgacgcgctc gaacgtgcgc tcctgcagca cgcgcagcag
     3481 cttgccctgg aggtcgagcg gaatctcggc gatctcgtcg aggaagatgg tgcctttgcc
     3541 ggcgagctcg aagcgacccg gccggtcggc gaccgcaccg gtgaacgcgc ccttcacgtg
     3601 gccgaatagc tcgctctcga gcacgccggg cgcgagcgcg gtgcagttga cggcgatgaa
     3661 tggacgctcg ttgccgcgag cgccacgcat cccgagctcg gccgcattgt ggatcgcacg
     3721 cgcgaccagt tccttgccgg taccggactc gccgcggatc agcacgggcg cgcgcgtcgt
     3781 cgagacggcg ccgatctgct tccacacgtc gcggatcgcc gggctcttgc ccaggatgtc
     3841 gccgacctcg gggccgcgcg acgccagctc gacgagctcg ccacgcgctt cgcggctcgc
     3901 gagcgcgctc ttcaccacgg agccgaggcg atcgatgtcg accggcttga cgaggtactc
     3961 gtacgcgccg agctggatcg ccttgacggt cgattgcatg tcatcgcgcg cggtgacgac
     4021 cacgacggtc ggcgcgtcat cgcgatcgac gagcgcgttc aacacgtcga agcccgagcc
     4081 cccgggcaag ccgaggtcga gcaacatcag atcgatcgga gcgccgctcg cgttggacgc
     4141 cgaggtagcg ccgctcgcgc tggacgccga ggtagcgccg tgcgtggccg agctcgccgt
     4201 tccgctcgct gcgacgttcg aattcaccgc ggcgatcgcg ccgggtgcgt cttgtgcggt
     4261 cgcgacctcg tagccttcgc cgccgaggaa cttctcgagc gtgcggcgaa tcgagcgatc
     4321 gtcgtccacg atcaggatgc gggccaccga ggtcactttc tgttgagagt ccacggtccg
     4381 tgccagcgca actcgtcgag atcgcatgga cggtccagtc gggagagtaa cgaaacgttg
     4441 cactggcggc gaaagaacgc tgctaccccg gccacttcga cgattcctat ttgacagtgt
     4501 tatatgacag tgttacatag aagacgtgag cgaaacctgg accttatcgg agcttgtcga
     4561 cgaagccgcg cagcggctcg aagcactgcc gccgccgaag aacggccagg tccgggcggt
     4621 cccggacgag cgaacgattc gctactacgg aacgatcgga ctgctcgatc ggcccgcggc
     4681 gatgcgtgga cgaacggcgc tgtactcgcg ccggcacctc gcgcaggtcg tcgcgatcaa
     4741 gcggatgcag gcagcgggtc acagcctcgc ggacatccag gcgatgtggt cgacgctcga
     4801 tgacacgacg cttggccgga tgtcgggcgt gcaggtcgcg accggcaagg cgaaggcagc
     4861 acgcaaggag ttctggaaac gggaggcaac aatgaccgag ctaccgccac cgagtgtgcc
     4921 gatgccacaa gcaccgacgg gaccgacgat tccgatgccg atgccgatgc cgacgccgac
     4981 gccgatgacg cccatgccag cgagcaacgc gccgcagatg ttgccgctcc ccacgttcac
     5041 gccatattca gcggcagggg cgggcaccgc gacgacgatc aacctgccac tcgagggtgg
     5101 cgtctcgatc gtgatcaccg tcgacgaaga cgccgctgtc tcactatctc ccgccgacgt
     5161 tcgcgcactt cgcgcggccg cggctccgct cgttaccgag ctcgcaaagc gcgggttcat
     5221 tggctcgtcg aaagccacac tggaggaagg accatgactg cgatgccgat tgtgttgatg
     5281 accgaggagg aggtggccgg ttatggccgc tcgagtgacg ccggctttgg cgcgctgaag
     5341 accgaacgcg gtctcttgcc gctggccgcg atggacgtgg atgcgcgcat cgcaggtgtg
     5401 gtcgcgtcga tcgagatcgc acagacgttc gtcaacacga cgggcgcggc gatcgaagcg
     5461 acgtacatct ttccgctgcc cgatcgcgcg gcggtccatc gcttccgcat ggaagtgaac
     5521 ggacgcgtga tcgaaggcgt catcgacgag cgtggcgctg cacgcgagca atacgacgaa
     5581 gcgatcgcgg cggggcatcg cgcggcgatc accgagcaag agcgcccggg cgtgttcacg
     5641 ctgcgcgtcg gcaacttgat gccgcgcgag tcggcgaccg tgcggctgtc gctcgtcggg
     5701 ccgctgcccg tcgacgacgg cgaggtcacg tttcagtttc cgctcgtcgt cgcgcctcgc
     5761 tacatgccag gcgctggcct cggcggtgat caggcgggcc tcggcatcgc cgaagacacg
     5821 agcttggttc ccgacgcatc gcggatttcg ccaccggtcc tcctccccgg ttgcccgaat
     5881 ccggtgcggc tcggcatgcg cgtgtcgctt gccgatcatc acgtgcgtga cgtctcgtcg
     5941 agcttgcacg cggtgacgac gacgctacgc gacgcgcagg tgatcgagat ccagcccggc
     6001 gagaagctga accgcgactt catcctgcgc tggcacatcg acggcaagga gctcagctcg
     6061 tcgctcgtgt gcagcgacga cgccgacggc aagggcggca cgtttgcact gacgctcgtg
     6121 ccaccgagca cgctcgcagt gtcgcagaaa ccgcgtgacg tcgtgttcgt gatcgatcgg
     6181 tccggcagca tgagcggttg gaagatgctt gcggcgcggc gcgcgaccgc gcggatgatc
     6241 gacacgctga cgtcgcgcga tcggttcatg gtgatcgcgt tcgacgacaa catcgaggtg
     6301 cagcccgagt ccaagctggc gcatgcgagc gatcgcacgc ggttcacggc gctcgaggta
     6361 ctcgccaaga tcgacgccga gggcggcacc gagctcgcgc agccgctgca gctcgcggtg
     6421 tcgacgctcg ccggcggtca cgatgatcgc gagcgcgtga tcgtgctcgt caccgacggg
     6481 caggtcggca acgaagatca catcctgcgc gagatcgcgc cgagcctgaa gaacgtgcgc
     6541 gtgttcacgc tcggcatcga taaggcggtg aacgccggct ttttgcggcg gttcgccggt
     6601 gcgggcggcg gcttgtgcga gctcgtcgag agcgaggatc gcctcgatgc ggtgatggca
     6661 aaggtgcatc gccgcatcgg cactccagtc gcgaccgagc tgcgcctcga gccgcgcggg
     6721 atcactgtcg acgcgagcac gatcgcgccg gcgaagctgc ccgacgtgta cgcgggcgcg
     6781 ccggttgtcg tgttcggccg ctacaagggc gcggctgcga gcgatgcatc gctcgcggtg
     6841 tcgggcacca cgtacggtga tccgatgaag cagaccgtcg cgcgtgacgc ccagtcgccg
     6901 ggcgcgtggc ttgccgcgag ctgggcgcgc gcgcacattc gcgagctcga ggacaagtac
     6961 gtgctcggcg cccgcgagct cgaaggcgag atcgtgcgcg tgtcgaagac gttcagcgtg
     7021 ctgtcgcggt tcactgcgtt cctcgccgtc gatcgcagcg aggtcgcgaa caagggcggc
     7081 aacgtcgtgc aggtcgtgca gcccgtcgag gagccggccg gctgggacat gaaggtcggc
     7141 ggtttcggcg gccagacgcg ccggcgtttg cccctcggca aggcacgggg cggtcgcccc
     7201 ggcgcggcga tgccaccgcc gatggcgtcg atgccgtcga tgagcgcgcc gatggcgaag
     7261 aagaccagca tgccgatcgg cggcatgagc gctgggccgg cgcgattcgg gccgatggac
     7321 gacatgaagg agatcacgcg cggcatcgcg ccgagtggac cgaacgcgtc accgccgcgc
     7381 ccaccgatgg cgccgccgct tccagctcct ccgccgccgc aagcgcagta cgcaccgatg
     7441 gtccacgaca aggcccgcga gaaggcggtg gccggcgatg cgtcggcgta cctcgcgaag
     7501 ctcgcgcagc tcgcgcgtgg tctgacgacc cacgcgattg ctggcgatgt cggggcgctg
     7561 cggttgctcc gccagcggct gaccgagtgg gtcgaagatc tgcgttcggt cggtggccac
     7621 gacgcgctcg ccgatgcggt cgagcacgag gtgaaacggc tgtcggtggc gctcgcgggc
     7681 accggcgatc tggcgaccga ggcgcgtgcg atcgcgaccg agctgtcgcg gctcgcgtcc
     7741 ggcgctacgc cgccgaagaa gagcggccgc gccgagttct ggaagtaaga ccgcggccgc
     7801 gtcgagctcg ggaaataaga tcgcggcctc gtggcagtag agcagagcgg ggcatcgagg
     7861 ctaagggttg tcccgtaact tatgccgcga cggtggagcc tcggtagtga attggatcgt
     7921 tgcggttccc cggacctcca ccgctgatcg cgacgacgcg gagatcgcga cgaggcacca
     7981 gcagctgtgc gttgcaggac catcggtagt tcttcgactt cgctgcacct gcgtggtcgc
     8041 gggtcggaat cagcgtgcca tcgacgatcc acgaccagcg ccgatcggcc ttcacatcga
     8101 cggcaagggc ggcaagtcgc ggtgtgatcg tggcaagaat ccgatgcgcc gacgacttcg
     8161 agatctgaag gatcgcggcg agctcacgta gggtcaggtc ggtgcgcagt gcgacgcatg
     8221 ccagaagaag gcggtgccgc agtggtcgcg accacggacg gcccatgcgt tgttcctttg
     8281 ggagttgccg catcaagatg tcgagtcggc gctcgctgat cgcagcaaga acatggatgc
     8341 gttggatcct cattgtcaga ggtgtcacgt aggatcgatt ggtggcgtcg tctggaaagc
     8401 ggacaagagg tgcgaacaaa cagacgccgt ggtcgcgaaa ccccgacgac ggcgtgtccg
     8461 tgctgcgcgt cggactcgac accagcgatc cacgccagcg cgcgcgtgtc gaggagatat
     8521 tctccgccgc gtactcgatc aagcgcgccg tccagcgcga cgctcgcgat cgctgccgcg
     8581 cctactggca agcgacgcac gagcgcgagc gcggaccgtc ggccgtgcgc gatcggctcg
     8641 ggctgtctcg caaggcgctc gagaacgccg cttacgcaca tctcaacggt gctccgcacc
     8701 tgcgacggtt cgcgaccaag gcactcgcga tgcacctcgc cgactccgtg tggaccgcga
     8761 ccgagcgtca cctgtttggc gatgcgcgcg gcaggcgcgc cggcgctccg cgcattggtc
     8821 gctggtatga cttccaatgg ctgccgggtc gcgctcggtc tcaccgcaga aagcgaaagt
     8881 gggagacgtt ccggttacac ggcacgctcg ccggccatcg cgccgcgtac acgcatcgcg
     8941 atggcgactt cgtccagccg cggcggcttc gccctgtcga gctcaccacc aagacgtgct
     9001 gggattacac agggccgctc gccgtcgtgt tcagtgggct tgccgacggg acgctcgtcc
     9061 ttcccgtccg acttccgacc gcgccgtcga accagcccat gctcgatcat cacctcgccg
     9121 acccgagcaa gtggcacaag gtcgacctgg tacggcatcg cgatccgaac gccgagggcg
     9181 gctggcgcta cgaagcccac ttgatggttc tgacgacacc ctatgtgtca ccggcgacgg
     9241 cggtgcggcg agccaaggtc gcgatcgagt cggtcgatcg gaccgcgggg atcgacgtca
     9301 acgtgtcatg cctcgcgatc gcctcgcacg acacgggcaa ggcgatgcgg ctgacccgcg
     9361 tcgagcggag tggcgaagac aagcagcgcg atcggggacg gcgacgacgc gagcgtcgtc
     9421 ggcaacgcga gctcgagcgg tcgcggcgcg ccgccaatcg cgcgcagtat cagctctcca
     9481 agcggcagga aaagcgagcc cgccggcgtg cggcccgcgg gctgccacct gtcgacgaga
     9541 ttccaatggg accgcgcaag gcacgggccg acggtgtgcc gctccagcgc taccagcgtg
     9601 accagctgtc ggcgcgcttt cgccggaccc gcgctgcaca agccgcagag gccgaggcgg
     9661 gcgctcgcgc gcggcgcgat cacgcgcgcg aggtcgcggc tggcgtcgtc gccacgcacg
     9721 gttatcagct cgtggtcgaa gactgcacca tcgcggcctg ggcgtcgtcg tggggcaccg
     9781 cggtcgccgc gtttacgccc gcgatgctcg tcgccgcgat cgatcgcgag gcgcgcgcgg
     9841 tggcggcgat cgccggcggc gagggtggcg tggcacgcgc caccacgcgc acgaccgcgc
     9901 tctcgcaaca ctgcccgtgt ggtgcgcggg tgcaaaagcg ccttgtcgat cgggtgcatc
     9961 gctgtccgga ctgcgggctc gtcggtgatc gcgacgatgt cgccgcggtg ctcgcatcct
    10021 ttgtcgtgca gatccagctc ggcgagccga cgtctgcacg cgtcgactac gccgccgcca
    10081 gcgatgcgct cgacgaaatc cggcgcgtct tcggttcttc gtaccagggg tggcaagaca
    10141 ccctgtccga gtcaaacgac ctctcagccc gcgatggatc gttcatcgcg tggacgacgt
    10201 ccacatccga ccctgtcgcg atggctcggc gaaacgtcgg cacggcatcg tgctcaaccc
    10261 tgaatgagcc tggcagccgc cagaccacgt cagaacgagc acgcgtgcga accgacaggt
    10321 ccacgaagta cgttcctcga tggacgtact tacgggacac ctcttagcgt tcggcgtccc
    10381 gtgctacacc gaggcgtgcg ctggtgcgtc gtggtgttcg tcgccgtgtt cgcagtatct
    10441 gcgtgctcga agcccgcacc gcatcgcgac gacgttcacg tcgtggcacc accggctgcc
    10501 gatgcgacgc ccgtagcggt cgtggccgat gccgccgtcg acgcgccgag cgtggcgatc
    10561 gtcgacgcgc cgatggtatg gagcgccgag cgcgagcagc tcacgctcga ctatcggcgt
    10621 gcgcatagcg atgcgaacgc gaaggacctc gcgatcgatc cgaaggtgat cgttctgcac
    10681 tacaccggcg gcaactcggc gaaggcaacg cgcacgtact tcgacaacgt gaagatcgaa
    10741 gcggcgcgca aggagctcgc gcggggcggt gcggtcaacg tgtcgtcgca cttcctcgtc
    10801 gatcgcgatg gcaccatcta tcgcctgcaa cccgagactc ggttcgcgcg ccactgcatc
    10861 ggtctcaacc atgtcgcgat cgggatcgag aacgtcggcg acgacaagca tccgctcacc
    10921 gatgcacagg tcgccgccga cgccgcgctc gttcgcgagc tcgcgcaccg ttttcacatc
    10981 acgcacctgc tcggccacta cgaggtgatg cggttccgcg atcatccgta ctacgtcgag
    11041 ctcgaccgcg cgtacaagaa cgagaaggac gatccaggcg ccgcgttcat ggcgcacgtc
    11101 cgtgagcgcg tcgcggacct cggcctcgcc ggactctagc ggctacttgc ctttcttgag
    11161 cgtgacgtgg tgcgaagcgc cgctccgcgg cgacaccttc tgcgtgtcct tcgcgtaacc
    11221 cggcttcgac agcgtgatcg ccgtcggttc gccctgcggt agcttgatcg tcgttggcgt
    11281 gacacccatc gacttaccgc ctgcggtgat cgtcgcgccg gccggcgtcg acgtgacctt
    11341 cacggacacc agcgtcctcg tgagcttgat gagcagcttg ttcgccttgc ccgccgtcgg
    11401 tgtgaacgcg cgcagcgtgt tcgggaacga cttcttcttg agcgagagct tcgactcggt
    11461 gccgcacggc agatcgatcg tgtccggcgt cgtgccgagc ttcttgctgt cgacatagat
    11521 gtctgcgccg gtcggcaggc tcttcacgtc gaccttgcac gggccgcttg cgaccggtgg
    11581 tgtcggctct ggcttcgcac tgcccgagcc ctgcgccggc gcggcggttt cgctgccgct
    11641 acccgcgggc gcgactgcga cctgttgtga gcccgagcct tgcggcggcg tgaccgcggc
    11701 cggtgcgtcg acggcaggcg gcggcgtgac cgtcatcttg ccgctcgcgg cgccggtgcc
    11761 ggtggtcgac gctgctttct tcttcgcgcc gccgagcgtg agcacgagca tcacgatcgc
    11821 gccgactgcg acgagtcccg ccgcgatcgc gagcaggagc atcttcttct tcttcgccgc
    11881 atcctgcggt gcggcggggt acgtgacgat gtccgtcgcg gacgggcgat acggctgctg
    11941 cgcgtacgca cccggatccg cgaacacgcc ggtccggcgc ggcggcatcg gttcgagcgg
    12001 gggcggcaga tcctgcggct tcgtgctgac gatctgcgcg ggttcgtcat ccggcagtgc
    12061 tttggcctcc atcagcgtcg agtcgatctc gatcttcgat tcgccgatgc cggtcggttt
    12121 ctcgtcccac tgcacgacgt cgagtggctt ctcgctcgcg accgcgagga cgggattgcc
    12181 ggccgttgcc gattccgcct tatcgacctt ctcgggctcg ctccagccgc cgcccgcggg
    12241 atcgagctgg atcgtccagt cgcccgtcgg cggtcccttc ggtggcggcg ccggcgcagg
    12301 cgttggctcc gacggcggcg caacggccgc gaccttcgct tccggcggta gtgcgtgcgg
    12361 taccgattcg tcgggcgaca tcgtccagtc gccggcggga atggcggcga ggatctcgga
    12421 cgcgcgcatg ccacccgagc gcgcgggcgg cgtttgcacg atcgcgccgc tgtccgatgg
    12481 cgaggagaca tcgacgggcg ccgtcactgt gggcagattg ccaatgtcgg ttggttcgtc
    12541 ttcgggcgca gccgacggag ctgccttcga cggcatccgc acgatcggca ccggtgccac
    12601 gggcgtcgga tgccgcggcg tcagcggagg tgtcgtcggc gccttgccgc gcggcgcggc
    12661 gggctgctgc gccttcgtgc tcttggggag cgtcggtggc tggggctcgg tttcggctgc
    12721 gtacttcgcc ggattcgccg gcggcacgat gcccagcggt tgggtctcga acacgtttcg
    12781 ccccacgacc gggaaaccga tcgatgtcgc cttgtgctgc ttcgacaact cgctgcgggg
    12841 cgtctcgacc ttcgtcggcg cgcttggctc gcgtgcagct gccaccggcg tcgcctgcat
    12901 cgtcggcgtt ggcggcgccg tcgttggcgg cgccgtcgtc ggcatcgggt cggtgacctt
    12961 tttcggcggc accgcgccaa tcgcggtggc ttcgtcttcg ccgatcggat cgaacagcgg
    13021 cttcgccgga atcgtcgccg gaatcggatc ggtctgtcgc ttcggcggac cctcgccgat
    13081 cgtcgtctgt tcgtcgtgca ccgtgagcgg tttcgtggcc gcgttatcgg gtgcgccggt
    13141 gccgggaacg gtggtgttgg gaatgaactt tggcggcgtc gcgtcggggc gtgccggttt
    13201 gccgtcggtg accggcgccg aggcggaggg cgccgacgac ggtgcggcga gcggcggcga
    13261 tgttggattc gccgccgcag gcgtcccgcg ctggggcgag agcatcgggt cgggcttgcg
    13321 atcgagcgcc ggcatgccga gcgtcgtcgc gttcgagacg acgcggccgg actcggtctt
    13381 cgccggcatc ttgtcgatcg ctggaaatcc gatcgacgtc agcttgccgg agttgtttgc
    13441 gaccttcggc gccggcaacg gtgtcgcggt cggatccggt ggcgcggtcg gcgtcttcgg
    13501 ccgcatttca ggcggcgtgc tcggcgtctt tggccgcatc tcgggcggtg cgctcggcgt
    13561 cttcggacgc ggcgtgccaa ccgccggaat cgatggcacc gcgaacttgc tgtcgccggc
    13621 gggacgcggc gcgttcgccg tcgcgatcag cgtcgctgga tcgccgatca ccacgcactc
    13681 ggcgagcgcg ttcgcggcgt cgatgcgccc gctcggcgcc ggcacgacgg ggcgcggctg
    13741 cgccgggatg tcggcaggac gcggtggcac gctcggcggg gccggcttca tcgccaggcg
    13801 cgccttctcg agctcgccga tcacaacttt gctcggctcg tccggctcga tgaacttcac
    13861 ggtcatcccg ggccgaccgt gcagaacggt gggcttcgca gacgactgca ggatctccgc
    13921 ctcgccttcg atcatcacgc cgccgtcctt gagcgtcagc gcgagtcgtc cgcgccgacc
    13981 tgccgggagt ggtgccgccg acgggacaaa caaccccgta cgatcggcgt agcggcgaaa
    14041 cgctgcgacg aactcctcgg ccgaaccgca agccgagacc aggtagaggg gaggcccgct
    14101 cgtcacctgt ggaaattgta ggcgaaaagt gccctggccg aaacgaacga cgggcacccc
    14161 agatgagatg cccgacgtcc aaatttccct ggtttggcag gtactacatg atcgcgaaat
    14221 cgtgcgcgcg aacggtcttg cggccgttct cgcttgcacg cttcgccgcc tgcgcgatgt
    14281 accagccgac gagcgcgttg agcccgtcga gcgcatcgcc agccgtgttg cagccggcgt
    14341 ccttgatcat gcccttcacc ttcgaggcaa cgagcagcat ctccttcgga ccaccgccgc
    14401 ccgacttacc cttcttcgct ttggccattt ctgttttctc ctgctgtttg aagcccgacc
    14461 cttttacacc gcccgcgagc cgagaaaaca cccaaatggc agaaaaatgc ccctagcggg
    14521 catttcattg ggatctaccg gtccagcttc acgacgaaca catctggctt cggtgccttt
    14581 gacagcaccg gatcattgag atcgatcttt tgctcgacgg gcggatcgag gagggccagc
    14641 ttgaagcgat agagcccggt cacgtaaacg ctgcccttgt cgtcgacggc aatcgcgcgg
    14701 ccctggtcgt ggtccttgtc gccgaacgtc gtcacccaca ccgtctcgcc cttcgcgttg
    14761 agcttcacga tgaagacgtc tttgttgagg ttcttcgtcg agatcgtgcc gcccttgccg
    14821 aaatcgaccg tgccctcgaa ccagccggtc acgatcgcgt tgcccacgct gtcgagcgcg
    14881 acgtcgtagc caacgtcgat gcgctcgccg ccgaacgtct tcgcccacaa gagcttgccg
    14941 tcgccggaaa accgcgcgac gtacacgtcg gcttcgccga gcgattggtg gtcatctttg
    15001 tcgccgaacg agatgccgcc cttgttctcg aacgagcccg cgatcgtcac gttgcccgcg
    15061 gggtcgacgg cgacgccgcc agcgacttca ttgaacgcgt tgccgaagcg ctgcgaccac
    15121 acgtgatcgc cgttgacgtc gtacttcgcg agcacgatgt cgttgtcgga accgccgccg
    15181 gccttcagat ccgagccgcc ccagttcgcg atgtccttga acaggccctg gacatagatg
    15241 ttgctgttcg cgtcgacggc gacgtgcatg atcgaatcgt cgtagttgcc gccgaattgc
    15301 ttcacccact cggtgtcgcc gctcgccgcg agcttgatca ggatcgcatc ggtgcggccc
    15361 ttgctcttca tgctcacgct cttcgcgccg gtcgtgatgt cgagcgagtc ggtgaacgag
    15421 ccgccgatca cccagccgcc gtcgggcatc gccgcgatcg tgttgacgcc gtcagagtcg
    15481 atgccgccgc cggtccatag ccagtcggcg ttgccgtcct tgtcgaacgc cgcgatgaac
    15541 aggtcgtcgg agccagcgct ctttttcgtg aacgagccga ccgtgatctg atcgaggaac
    15601 gtgccggtga cgacgacctt gtctcccttc accgcgatcg cattcgccgc gtcgtcgcgc
    15661 gtcgcgcccc aggtctgcgc ccatgcgatc ttgccgtcgg gaccaaactt cgcgacgaat
    15721 gcatccgact tgccggccga cttcttcttg ccgatgctgc cgccaaaatc ggtctcgcct
    15781 tcgatgtagc cgcagatgta gaccgagccg tcggcgccga ccgcgatgcc gcgcggcgag
    15841 tcgatgccga ggccgccgaa gcctgcctgc cacaccggct tgccggtcgc gccacctgga
    15901 tccgccgcga gcgccggcag cgccttgttc ggcacttctt ttttcggcgc gacgttcacc
    15961 gtcgtttcgg tcttggtttc gaccttggct ttcttctcac ctttgctgca cgcaacgggc
    16021 agcgcggcac acgcaacgac gacggcaatc gaggacggac gcatgggctg acgatgtcac
    16081 gcgtcgcccg cgccatcaat gtaggatgcg cccgtgctgc gctacgcgat cttcgttgca
    16141 accgctttgc tcgtcggttg tccaagtaaa cagccggagg ctccgcattc gcccgaggac
    16201 accgcgctgc gcattcgcat tgcgcaagcg gaggcgcatc gtgccgccgg tgtcgacgag
    16261 cttgtacagc tcgcgacgag tggcgacgtg caccagcgcg agctagcgct gcgtgggctc
    16321 ggccgcgtcg gtggcgacaa ggcgcgcgcg acgctcgaag catcgctcgc agacaaggat
    16381 ccgaaggtcg tcgcggcagc gtgctacgcg atcggcgttg cagcggcgct cgacgaaccg
    16441 ccggactgga aaccgggcgg caagctcgtc gccgtgctcg cagtcgcgcc gctgcccgca
    16501 ctcgaggcgc tcggtcgcgc gggcgatgcg acgaatcaac cggcgctcac cgcgaagctc
    16561 gccgacaagg atccggcgat tgcagcggca gcggcgatcg cgctcggtcg ccacggcagg
    16621 cgcaagatcg cgtggtcggc cgaggcgcgc gcggcgctcg cgaccgcgac gagccatgcg
    16681 gattcgaacg tgcgctacgc ggcgacgtac gcgctgtcgc gcgagcacga gccgcccggc
    16741 gacgacgcgg tgaatgcggc gctgatcgcg cggattgccg acgagtatcc cgagacgcgc
    16801 gcgagtgcga tcgcgggcct cgcaaagcgc aaagcggtca ctgcggcgcg caagcagatc
    16861 gtcgagtcgc tgcgcgatcg cgactggcgc gtcgccgtcg aggcagtgcg cgcgctcgcc
    16921 ggtgcgaacg gcgacgacga aggacgcgtc gccgtcatcg gcagcttgtc ggtgcgcgca
    16981 gcccagctcg agaaaggaag cgcaaccgag gcgcaggtca tcgtcgaagc gctgcgaacg
    17041 ctgctcgacc atccgataag taacccgaag tcaattgcaa ctgaattgca tatcgtctcg
    17101 gagccggcga atgcgtcgat tccggcgctc acgacgggct ggagcgagtg tctccagcaa
    17161 gctgtacagg ccagcgcgac gagcggtgcg ccgacgggca cgacaagggg tgcgccgacg
    17221 ggcacggcga gcggtgcgcc gacgggtacg aagagcggtg cgccgacggg cacgacgagc
    17281 ggtgcgccga cgggcacgac gagcgctggc gccatcgagg cgctgacgac ctgtgggcac
    17341 ggcgcgcttc ccgatcatct gcgcctgccg ctgctcgccg atgtggcgaa gggcggggac
    17401 atcgcagcga agcgcgctgc gctgcgcgtg ctgctcgcgc acgacgatcc gcgcgtgcgt
    17461 gcggcgggcc tcaacctgtt gcccgagacg tggaaagaca gcgacgcgaa agggcaggcg
    17521 acgatcgtcg gcaccatatc gtcggcgctc gcggtgaaga atcccatcgt cgccggcacc
    17581 gcggtcgatg tcgccggcac ggtctacgag cagctcccca aggacgacgc gctgcgcacg
    17641 acgctcgata acgcgatcct ggcgcgcgcg aagaccgaga ccgacgtcga gctgtcggcg
    17701 agcctgtaca ccgcgatcgg caagaacgcg attcccggcg gcgccgacgt ctgccgtggc
    17761 gggctcgccg ctgcccccgc gcgcgcgaag gccgcggccg attgcttgaa ggcgctcggc
    17821 gaagctgcac caccgcaagc cgctgcttct gccgagctgc cgcccgtcga tgtcgcgacc
    17881 gtgatcggca agaagctgcg ctggcacctc gtcacgtcgc gcggcgagat cgtgatcgag
    17941 ctacgtcccg acgtcgcgcc gtgggcggtc gcgtcgatcg tcgcgctcac cgaccgcggc
    18001 ttctacaacg gcctcgagtt tcaccgcgtg gtcggcaact tcgtcgtcca gggcggcgac
    18061 ccgacgcaat cggggtgggg tggaccgggc tacacgctgc cggcggagcc cgcgagcact
    18121 tttgacggcc cggggtacgt ggtcggcggt gtaggtatcg ccgatgcggg caaagacagt
    18181 ggtggttctc agtggttcat catgcactcg cgcgcacctc acctcgacgg gcggtacacg
    18241 tggatcggga gcgttgtcga cgggcagaag tacgcggatg cactcttgat tggggatcgg
    18301 gtcgagcgcg cttccgttga gcgatagatt cgcggaactg ctcggaaacg agcgttgtcg
    18361 tgattgcatg caaaatagat cggcggctcg tgtcgttcgt tcgaggaacc acatgcgcat
    18421 gcgaccactg tttgccgccg tccttccact gggtatcgca actgccgcgt tcgcagagcc
    18481 ggcgcttgcc aaacgcatga tcagcgattt cggagcgccg agctttcaac tgctcgggcg
    18541 cccgatcggc ggagcaaaac ccgcccgcga aacaccggcg tatctcgccg gtcaacgcat
    18601 cgcggccgtc ggtgatggcg cactcgtcat cgactccgac agcggtgatt tgatcaagac
    18661 cgacaaggcc ggcacccgca tcgcgtcgat cgcgatcggc aagaacgcgg gcctgatggt
    18721 ctacgacccg accgcgaagc tcgcctacgt cgtcgatcgc ttggcgagca aggtgaaggt
    18781 cgtcaaggtc ggcgagaggc tcgagatcgc cgcgacgcac gcgacgccag ccgagccgta
    18841 tggcatcgcg ctgtcgccgg atcgcaagac ggtgatggtg acgacgatcg ccgatcgcac
    18901 gctggtcgcg ctcgataccg ccaatggcaa ggagaagtgg cgcaccgcgc tcggtcgcga
    18961 gccgcgtggc atcgcggtgt cgcccgacgg cacgcgcgcg ctcgtcgcgt acctgacgac
    19021 cggcacggtc gatcaagtcg acctgctcga gagccacaag gccgagcaca tcgcgctgtc
    19081 gacccagaac atgcgccgcg gtcgcttcgg cggcggcgat gcattcgcgc gcggcgcgtt
    19141 caccgcgctg ttcctcggcg accaggaagc ggtcgttccg ttccagcgcg agacgccggt
    19201 ccaggcgatc ggtggcggcg agcgcactgg c
//
LOCUS       DASUFX010000228         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1563997, whole genome shotgun sequence.
ACCESSION   DASUFX010000228 DASUFX010000000
VERSION     DASUFX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563997"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFV99_08960"
     CDS             <1..507
                     /locus_tag="VFV99_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059476.1"
                     /translation="PVPTIPIDATPPVDEGMAYLIVRTIPDGGTIKVGDQSRHATRQP
                     GDPTGATTAQLVLPPGKHVVTAELAGFQSEQRTVVLAAGDNQRIEITFTKKTTGRPTP
                     MGKLTVRTTPWSDVYLGTRKLGQAPFADLELPVGTHTLTFKNPSRPTVTKTVTIKAGK
                     SVKLNFNL"
     gene            702..1250
                     /locus_tag="VFV99_08965"
     CDS             702..1250
                     /locus_tag="VFV99_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5059477.1"
                     /translation="MHKVWTYADLRHTPDDGKRYEIFDGELVVTPAPGIPHQVTVFEL
                     TMRLYLRLKEIAIAYISPVDVLLAWDRVLEPDIVVVRHERQAIVTERAIEGAPDLVVE
                     VLSPRGATRDRVRKARLYANSGVPEYWIVDPRAKTIEVYALGDRGYCLDGEYAVGDWL
                     TSVTFDFTLDVSSIFTFKAPAQ"
     gene            complement(1231..2226)
                     /gene="glpX"
                     /locus_tag="VFV99_08970"
     CDS             complement(1231..2226)
                     /gene="glpX"
                     /locus_tag="VFV99_08970"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5059478.1"
                     /translation="MQDRNLALEVVRITEAAALASARLMGRGDRKLADHVAVEAMRVA
                     FDAIDIRGTVVIGEGERDEAPMLYIGEQVGHGWKDGEESPKVDIAVDPLEGTNLCATG
                     EPDAISVIAIADDGQFLNAPDTYMQKIAVGPEARGVIDMRESPTWNLQRIAKAKGKKV
                     QDCTAVILDRDRHNDLIGEVRQAGARIRLIGDGDVSGAVMTANPDSGIDVLFGTGGAP
                     EGVIAAAALKCVGGDIQGRLRFRSDEEKARAKRMGVKDEDFIYGLEDLAKGHVMFAAT
                     GVTKGYLLDGVRFFGGGAKTESIVMRSKSGTVRVINATHHFNTKPKYSWATGLEP"
     gene            complement(2302..2583)
                     /locus_tag="VFV99_08975"
     CDS             complement(2302..2583)
                     /locus_tag="VFV99_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059479.1"
                     /translation="MVPGGDLTWTGSEALMTTQTTTSNHLSRLDLTTGAATDLGDTTY
                     DSVTGLAWFDGALFGFANAGTIFVIDPATGAPTRTQSTTEMWAGATTGD"
     gene            complement(2618..3376)
                     /locus_tag="VFV99_08980"
     CDS             complement(2618..3376)
                     /locus_tag="VFV99_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5059480.1"
                     /translation="MRGWLPLMLVLLGGCDALFGLDDLHTGPYFDAPPDRDFRDVRAI
                     GDGMSDPRLLARFDFEDNYEDAVSGQAATCVSAAATCPGFTDGVHGRALLLDGVDDCL
                     QFPLPSLPMHFTIAVRIYRNDDVPETAVAKPYASTAFDSWQIDTAMVRQLRYVTYNGS
                     AERALLFDDVVEPGAWLHVAVTYDGGMKAAYVNGSALTSSSVGEGVITDGSAMLIGCD
                     RDSAQYSRMFSGRLDEVVVYDEELGPPEIAALAQ"
     gene            complement(3389..3787)
                     /locus_tag="VFV99_08985"
     CDS             complement(3389..3787)
                     /locus_tag="VFV99_08985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5059481.1"
                     /translation="MMRHRYEYQIIFGDTDQMGVVYYANYLRFFEGARASYWRALGRS
                     YKDLEDAQVALPVVEAHCNYKRPALYEDVIVVETHIGEVRAASLRFLYEVRRGHELLA
                     EGYTRHAVIGPGGKPRALPDFMRDLITRGS"
     gene            complement(3784..5082)
                     /locus_tag="VFV99_08990"
     CDS             complement(3784..5082)
                     /locus_tag="VFV99_08990"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5059482.1"
                     /translation="MSRTIGVALLGLGNVGGGVVKLLEDNAAAIEARLGARIALRGIA
                     VRDPAKAKRVVDVDRTLLTTNLDGLIKRPDVDIVCELIGGTTEAKRVVLSAIESGKQV
                     VTANKALLAECGAEVFGAAEAAGVDVYYEAAVCGGVPIIRVLREGLASDKVEALWGIV
                     NGTSNYILSTMTETRRPFNDILADAQRLGYAEADPTLDIGGGDAAHKLAILVSLCFGT
                     SVDVNAIAKDGIDVVEPIDLAYAEKFGYVIKPLVIARDHGTAVEARVHPALVPHNWLL
                     ADVSGAKNAVYVQSYALGPSMYYGAGAGMLPTAMSVVSDIIEIGRNIFARAAGGARPV
                     RPRPAVARPLLPLADIRAKYYLRFGVVDEPGVLGKLMTVLGSCSVSIEQVVQDTKSVD
                     PVAVVVLTHEAREGDIRRALAEIDRLAVVKTPARVIRIAG"
     gene            complement(5082..>5568)
                     /locus_tag="VFV99_08995"
     CDS             complement(5082..>5568)
                     /locus_tag="VFV99_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5059483.1"
                     /translation="RMRHGVAFVIALVLAGCPRGSEGTTPRRPATPKEVVASARATIE
                     QWRQAYEIRSFDALAKLYAHDIDLVVVQEGMPLVGWSSVERMLKDRIARYKEVHVRLK
                     DIQVQSLAPTAAVATAAMTRELGDGITTVSESGALTIVLRQEGDAWLIVTEHYSFKRG
                     G"
BASE COUNT          937 a         1861 c         1817 g          953 t
ORIGIN      
        1 ccggtgccga cgattccgat cgacgcgacg ccgcccgtcg acgaaggcat ggcgtatctg
       61 atcgtacgca cgattcccga cggcggcacg atcaaggtcg gcgatcagtc ccggcacgca
      121 acgcgacagc ccggcgatcc gacgggcgcg acgaccgcgc agctcgtgtt gccgccgggc
      181 aagcacgtcg tgaccgccga gctcgcaggc tttcagtccg agcagcggac cgtcgtgctc
      241 gcggccggtg acaaccagcg catcgagatc acgttcacga agaagaccac gggacggccg
      301 acgccgatgg gcaagctcac cgtgcgcacg acgccgtgga gcgatgtcta tctcggaacg
      361 cgcaagctcg gccaggcgcc gttcgcggat ctcgagctgc cggtcggcac gcacacgttg
      421 acgttcaaga acccgtcgcg cccgacggtg acgaagaccg tcacgatcaa agcgggcaag
      481 tcggtgaagc tgaacttcaa cctttagacg ctcgctgttt cgcggaaagg gcgacgccct
      541 cgcctgtcgt gaggcccgac gaaccagtcg gtcgtgatgg cagccccgga cgccgtcagc
      601 cggccctcgg acgatcacgg acgcgcgggc gcgtagtttc cgtcggttgc gagcggccgg
      661 ctccttgctc gaagcgtgcg cggtatgctg gaggcacgac gatgcacaag gtctggacct
      721 acgccgatct caggcacacg ccggacgacg gcaaacgcta cgagatcttc gacggagagc
      781 tcgtcgtgac gccggcgccg ggaattccgc accaagtgac cgtcttcgag ctcaccatgc
      841 ggctctatct tcggctcaag gagattgcga ttgcctacat ctccccggtc gacgtgctgc
      901 tcgcctggga ccgcgtgctc gagccggaca tcgtggtcgt tcggcacgaa cgacaggcaa
      961 tcgttaccga gcgtgcgatc gaaggcgcgc cggatctcgt tgtcgaagtt ctgtctccgc
     1021 gaggagcaac gcgcgaccgg gtgcgcaagg cgcgccttta cgcgaacagc ggagtaccgg
     1081 agtactggat cgtcgacccg cgcgcaaaga cgatcgaggt ctatgcgctc ggcgatcgcg
     1141 gctactgcct cgatggcgag tacgctgtcg gtgactggct aacgagcgtg acgttcgatt
     1201 tcacgctcga cgtatcctcg atctttacgt tcaaggctcc agcccagtag cccaggagta
     1261 cttcggcttc gtgttgaagt ggtgggtcgc gttgatcacg cgaaccgtgc ccgacttcga
     1321 acgcatcacg atcgactcgg tctttgcgcc gccgccgaag aatcgcacgc cgtcgagcag
     1381 gtagcccttc gtcacgccgg tcgccgcgaa catgacgtga cccttcgcga gatcttcgag
     1441 gccgtagatg aagtcctcgt ccttgacgcc catccgcttc gcgcgcgcct tctcctcgtc
     1501 gctgcggaag cgcaggcggc cctgaatgtc gccaccgacg cacttgagcg cggccgccgc
     1561 gatcacgccc tcgggcgcgc cgccggtgcc gaacaggacg tcgataccgg agtcgggatt
     1621 cgcggtcatc accgcgcccg agacgtcgcc gtctccgatc agacggatgc gcgcgccggc
     1681 ctgacggact tcgccgatca gatcgttgtg gcgatcgcga tcgaggatga ccgccgtgca
     1741 atcctgcacc ttcttgccct tcgccttcgc gatgcgctgc aggttccagg tcgggctctc
     1801 gcgcatgtcg atcacgccgc gcgcttcggg gccgaccgca atcttctgca tgtacgtgtc
     1861 gggcgcgttg aggaactggc cgtcgtcggc gatcgcgatg acgctgatcg cgtcgggctc
     1921 gccggtcgcg cacaggttcg tgccctcgag cgggtcgacg gcgatgtcga ccttgggcga
     1981 ttcctcgccg tccttccagc cgtgaccgac ctgctcgccg atgtagagca tcggcgcttc
     2041 gtcgcgctcg ccctcgccaa tgacgacggt gccgcgaatg tcgatcgcgt cgaatgcaac
     2101 gcgcatcgct tcgaccgcga cgtgatcggc gagcttgcga tcaccgcgtc ccatcaggcg
     2161 tgccgacgcg agtgcggcgg cctcggtgat gcggaccact tcaagagcga ggttgcggtc
     2221 ttgcatgcgg aaaggtgtac gacgcgatcg ggcgtggatc aatgcgcgga cgggcgcgac
     2281 tcgccctgcc ctcacgatct ttcagtcgcc cgttgtagcg ccggcccaca tttcggtcgt
     2341 ggattgggtg cgggtcggcg cacccgttgc gggatcgatg acgaagatcg tgccggcatt
     2401 cgcgaatccg aacagcgcgc cgtcgaacca ggcgagcccg gtgaccgagt cgtacgtggt
     2461 gtcaccgagg tcggtcgccg caccggtcgt gaggtcgagg cgagataggt ggttgctcgt
     2521 cgtcgtctgc gtcgtcatca gcgcctcgct gccggtccat gtcaggtcgc caccgggaac
     2581 caccccgatc ggaccgatca gctgggacgc ggttcggtta ctgggcgagc gctgcgattt
     2641 cgggcggacc gagctcctcg tcgtagacga cgacttcgtc gagacgtcca ctgaacatgc
     2701 ggctgtactg cgcgctgtcg cgatcgcatc cgatcagcat cgccgagcca tccgtgatga
     2761 ctccttcgcc gacggacgag gacgtcaatg ccgagccgtt gacgtatgcc gccttcatgc
     2821 cgccatcgta ggtgaccgca acgtgtagcc acgcccccgg ctcgacgacg tcgtcgaaga
     2881 gcagcgccct ctcggcgctg ccgttatagg tcacgtagcg cagctgacga accatggcgg
     2941 tgtcgatctg ccagctgtcg aaggctgtcg acgcgtacgg cttcgcgacc gccgtctcgg
     3001 gaacgtcatc gttgcggtag atgcgcaccg cgatcgtgaa gtgcatggga agcgacggca
     3061 gcgggaattg cagacagtca tcgacaccgt cgagcaggag cgcgcggccg tggacgccat
     3121 cggtgaagcc gggacacgtg gccgccgccg acacgcacgt cgcagcttga ccgctgaccg
     3181 cgtcctcgta gttgtcctcg aagtcgaagc gcgcgagcag ccgcgggtcg gacatcccgt
     3241 caccgatcgc acggacatcg cggaaatcgc ggtccggcgg ggcatcgaag tacgggcccg
     3301 tgtgcaggtc gtcgaggccg aatagcgcat cgcagccgcc gagcaagacg agcatcagcg
     3361 gcaaccaccc ccgcatcacc tcatcgtatc agctaccgcg tgtgatcagg tcgcgcatga
     3421 agtcgggaag cgcgcgcggc ttgccgccgg ggccaatcac cgcgtgtcgc gtgtagccct
     3481 cggcgagcag ctcgtggcca cggcgcacct cgtagagaaa ccggagcgac gctgcgcgca
     3541 cttcgccgat gtgtgtctcg acgacgatca cgtcctcgta cagcgccggc cgcttgtagt
     3601 tgcagtgcgc ttcgacaacc ggcagtgcga cctgtgcgtc ctcgagatcc ttgtagctgc
     3661 gaccgagcgc acgccaatac gacgcgcgtg cgccctcgaa gaagcgcaga tagttcgcgt
     3721 aatagacgac gcccatctga tcggtgtcgc cgaagatgat ctggtactcg tagcggtgcc
     3781 gcatcatccg gcaatccgaa tcacgcgcgc gggcgtcttc acgacggcga gccgatcgat
     3841 ctcggcgagt gcacggcgaa tgtcgccttc gcgcgcttcg tgagtcagca cgacgacagc
     3901 gactggatcg acggacttcg tgtcctggac gacctgctcg atcgacaccg agcacgaacc
     3961 gaggaccgtc atcaacttgc cgaggacgcc gggctcgtcg acgacgccga agcgcaggta
     4021 gtacttcgcg cggatgtcgg caagcggcaa cagcggccgg gcgacggcag gacgcggacg
     4081 taccggacgt gctccgccgg cggcgcgcgc aaagatattg cggccgatct cgatgatgtc
     4141 gctgacgacg gacatcgcgg tcggcagcat cccggcaccg gcgccgtagt acatcgacgg
     4201 gccgagcgcg tagctctgca cgtagaccgc gttcttcgca ccgctgacgt cggcgagcag
     4261 ccagttgtgc ggcacgagcg cgggatggac gcgcgcctcg accgccgtac cgtgatcacg
     4321 cgcgatcacg agcggcttga tcacgtagcc aaacttttcg gcatacgcga gatcgatcgg
     4381 ctcgacgaca tcgatgccat ccttcgcgat cgcgttgacg tcgacgctgg tgccgaagca
     4441 tagcgagacc aagatcgcga gcttgtgcgc ggcgtcgccg ccgccgatgt cgagcgtcgg
     4501 gtcggcttct gcgtaaccga gtcgctgcgc gtcggcgagg atgtcgttga acggccgccg
     4561 cgtctcggtc atcgtcgaca agatgtagtt cgacgtgccg ttgacgatgc cccacagcgc
     4621 ctcgaccttg tcgctcgcga ggccttcgcg caggacacgg atgatcggca cgccgccgca
     4681 caccgcggcc tcgtaataca cgtcgacgcc ggccgcctcg gcggcgccga acacctcggc
     4741 accgcactcg gcgagtagcg ccttgttcgc ggtgacgacc tgcttgccgc tttcgatcgc
     4801 gctcagcacc acgcgcttcg cttcggtggt gccgccgatg agctcgcaca cgatgtcgac
     4861 gtcgggccgc ttgatgagcc cgtcgaggtt cgtcgtcaac agcgtgcgat cgacatcgac
     4921 cacgcgtttt gccttcgccg gatcgcgaac cgcgatcccg cgcagcgcga tgcgcgcacc
     4981 gagccgtgcc tcgatcgccg cggcattgtc ttcgaggagc ttgaccacgc cgccaccgac
     5041 gttaccgagc ccgaggagcg cgactccgat cgtgcgcgac atcagccacc tcgcttgaac
     5101 gagtagtgct cggtcacgat cagccacgca tcgccttcct ggcgcaagac gatcgtgagt
     5161 gcaccggact cgctgaccgt ggtgataccg tcgccgagct cgcgggtcat cgcggcggtc
     5221 gccactgcag cggtcggcgc aagcgactgg acctggatgt ccttgagacg cacgtggact
     5281 tctttgtagc gcgcgatccg gtccttgagc atgcgctcga ccgagctcca cccgaccagc
     5341 ggcatgcctt cttggacgac caccagatcg atgtcgtgcg cgtacagctt ggccagcgcg
     5401 tcgaagctgc ggatctcgta tgcctgccgc cactgctcga tcgtcgcgcg ggcgctggca
     5461 accacttcct tgggcgttgc cgggcgtctc ggcgtcgtgc cttcgctgcc gcgcgggcaa
     5521 cctgccagga cgagcgcgat gacaaacgca acgccgtgtc tcatgcgc
//
LOCUS       DASUFX010000229         4624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1423627, whole genome shotgun sequence.
ACCESSION   DASUFX010000229 DASUFX010000000
VERSION     DASUFX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423627"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFV99_09000"
     CDS             <1..615
                     /locus_tag="VFV99_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059484.1"
                     /translation="EPPPAAPAGGVPNTSAPAIGPTGAPATKPGAAPVAGAQPQQVSI
                     PGATAPATKQGALMDRKLVKPSAELAAIKFDLEPNWDRDYGEAGTFSLVVKVPNTEES
                     RVFAAHYGYEEQSAPFDCDQYRKFLEDKKIMTVTLDRQRGAACYIEGKEPNGVLAYRY
                     LVNYGGKRLLCNGSLYKDSASTALGDLRDKVLMQAKKICETLAL"
     gene            653..2608
                     /gene="dnaX"
                     /locus_tag="VFV99_09005"
     CDS             653..2608
                     /gene="dnaX"
                     /locus_tag="VFV99_09005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5059485.1"
                     /translation="MSYLVLARKYRPTTFSEVVGQEHVTRTLGNAFATGRVHHAFLFC
                     GPRGCGKTTLARIVGKALNCEKAPKNSVGTAEPCGECSACTSIANGSAVDYQEMDGAS
                     NRGIDAIRELTEAVRYQPAVLRKKVYVIDEVHMLTTEAFNALLKTLEEPPPHVTFVLA
                     TTEPHKLPNTILSRCQRYDFKLVPASRLAQHLASIFKQEKLKIEAGAISLVVRESGGS
                     VRDALSLCDQIISYVGDDKITEAHVAEVLGVADRSLTRTLVKSLANGDAGTALAAVES
                     AIERGVDEVQLARAIVRYLRDLAVLQVAPNKPELIDASDEERAELTEEAGQIDRTRVT
                     QMFDRMLRCCDELGKTLQPRLVLDCALIDVATIEPLVPLGDLIDRLGELEARVAKGGG
                     GPRPTSPPAGGARAPKMTAPHTPSSKGKTMSMGAGESAPSSARANETSAPVRTEAPRV
                     ESAPVPRSVPEAVPATQSAPTPYSGSLSSPVAMQPSGPVPKAETVASGSGPNPPTIAV
                     PSSQHEALAAWAKVIDYLEEQRKISLRGYYEFARVLKWTATDLELGFAADDDSKWAGE
                     NAGEQANINELRSVLADLGHKVKVTVRMLDATESQATQVRSIVESTREKSSAERTKRE
                     QEAREHPITKHVLQAFGASIKEIKTDV"
     gene            2601..2939
                     /locus_tag="VFV99_09010"
     CDS             2601..2939
                     /locus_tag="VFV99_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5059486.1"
                     /translation="MSNGKMPDLNALMKQAQKLQGDVQKVQEELAQMQCDGAAGGGLV
                     TATVNGQFEVVRVKIDKSVVDPNDVGMLEDLITAAINAAGTRVREVSKEKMASVMGGM
                     GGMPGMPGLF"
     gene            2940..3536
                     /gene="recR"
                     /locus_tag="VFV99_09015"
     CDS             2940..3536
                     /gene="recR"
                     /locus_tag="VFV99_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5059487.1"
                     /translation="MAADPIRRLVQELSRLPGIGEKTATRLAFHLVRGNRQQVLDLAQ
                     ALVDVTDKIRLCSVCMNMTEADPCTLCADPRRDGEVICVVATPSDLIAIDRGGHFRGR
                     YHVLHGLLSPLEGIGPDDIRVAELVRRLGGDPPVREVIIATSPSVDGEATAMYVARVL
                     KPLGVKVSRIATGLPVGGELEYSDQATIARALAGRAQM"
     gene            3663..4124
                     /locus_tag="VFV99_09020"
     CDS             3663..4124
                     /locus_tag="VFV99_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5059488.1"
                     /translation="MYEEELAQINHVCEVLHRDANARAVLVIDKNGQAFAQAGEVEQL
                     DVTSLSSLTAGNVAATGAIASLLAEKEFAGQFHEGEKTNVHISIVGQRVILVVLFDER
                     SSLGLVRLRVRKATGELVQVLDVLTKKSQSGSQPSVFSEITDDDIDNLFND"
     gene            4134..>4624
                     /locus_tag="VFV99_09025"
     CDS             4134..>4624
                     /locus_tag="VFV99_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX5059489.1"
                     /translation="MSFINYSSREINCKIVYYGPGLCGKTTNLQYIYNKTNPEAKGKM
                     ISLATETERTLFFDFLPLSLGEIRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVYV
                     GDSQIERMEANIESLENLRSNLQEQGYDLDKLPYVVQYNKRDLPNAIPLPELEEALNP
                     TKVP"
BASE COUNT          894 a         1456 c         1538 g          736 t
ORIGIN      
        1 gagccgccac ctgcggcgcc cgccggaggt gttccgaata cctcggcacc ggcgatcgga
       61 ccgacggggg ctccagcgac gaaaccgggc gccgctcccg ttgccggcgc acagccgcag
      121 caagtgtcga ttcccggcgc gactgcaccg gcgacgaagc aaggcgcgct gatggatcgc
      181 aagctcgtga agccgtcggc ggagctcgct gcgatcaagt tcgatctcga gccgaactgg
      241 gatcgcgact acggcgaagc gggcacgttc tcgctcgtcg tcaaagtgcc gaacacggaa
      301 gagtcgcgcg tgttcgcggc gcactacggc tacgaagagc agagcgcgcc gttcgactgc
      361 gaccagtacc gcaagtttct cgaggacaag aagatcatga cggtcacgct cgaccgtcag
      421 cgcggtgcgg cctgctacat cgagggcaaa gagccaaacg gcgtgctcgc gtatcgctat
      481 ctcgtgaact acggcggcaa gcggctcttg tgcaacggat cgctctacaa ggattccgcg
      541 tcgacggcgc taggtgatct ccgcgacaaa gtcctgatgc aggccaagaa gatctgcgag
      601 acgttggcgt tgtagcgacg gacccaccgg gtatagtgcc cgccggtccc gcatgtccta
      661 tctcgtcctt gctcgcaaat atcggccgac cacgttctcc gaagtagtcg gtcaggagca
      721 cgtgacccgc acgctgggca acgcgttcgc gaccgggcgc gtgcaccacg cattcttgtt
      781 ctgcggcccg cgcggctgcg gcaagaccac gctggcgcgc atcgtcggca aggcgctcaa
      841 ctgcgagaag gcgccgaaga atagcgtcgg taccgcagag ccgtgcggcg agtgcagtgc
      901 gtgcacgagc atcgcgaacg gcagcgccgt cgattaccag gagatggacg gcgcgagcaa
      961 tcgcggtatc gatgcgattc gcgagctcac cgaagcggtg cgctaccagc ccgcggtgct
     1021 gcgcaagaag gtctacgtca tcgacgaagt ccacatgctc accaccgaag cgttcaacgc
     1081 gctgctcaag acgctcgaag agccgcctcc gcacgtgacg ttcgtgctcg cgacgaccga
     1141 gccgcacaag ctgccgaaca cgatcctgtc gcggtgccag cgctacgact tcaagctcgt
     1201 gccggcgtcg cgcctcgcgc agcatctcgc gagcatcttc aagcaagaga agctgaagat
     1261 cgaagcgggc gcgatcagcc tcgtggtccg cgagtcgggc ggtagcgtgc gcgatgcgct
     1321 gtcgctctgc gaccagatca tttcgtacgt cggcgacgac aagatcaccg aggcgcacgt
     1381 cgcagaagtg ctcggcgtcg ccgatcgctc gctgacgcgc acgctcgtga agtcgctcgc
     1441 gaacggcgat gccggcaccg cgctcgcggc ggtcgaatcg gcgatcgaac gcggcgtcga
     1501 cgaagttcaa ctcgcccgcg cgatcgtgcg ctacctgcgt gacctcgcgg tgctccaggt
     1561 cgcgccgaac aagccggagc tcatcgacgc gtcggatgaa gagcgcgccg agctgaccga
     1621 agaagccggc cagatcgatc gcacgcgcgt cacgcagatg ttcgatcgca tgctgcggtg
     1681 ctgcgacgag ctcggcaaga cgctgcagcc gcgcctcgtg ctcgactgcg cgctgatcga
     1741 cgtcgcgacg atcgagccgc tcgtgccgct cggcgatttg atcgatcggc ttggcgagct
     1801 ggaagctcgc gtcgccaagg gaggcggcgg gcccaggccc acatcgccgc cggctggtgg
     1861 agcgcgcgcg ccgaagatga cggcgccgca cacgccgtcg agcaaaggca agacgatgag
     1921 catgggggcc ggcgaaagcg cgccgtcgtc ggcccgtgca aacgaaacga gcgcgcctgt
     1981 ccgtaccgaa gcacctcgcg tcgagagcgc tccggttcct cgatcggtac cggaggcggt
     2041 gccggcgacg cagagcgctc cgacaccata ttccggctcg ctgagctcgc cggtggccat
     2101 gcagccgagc ggtccggtgc cgaaggcgga gacggtcgcg tcgggaagcg gaccgaatcc
     2161 gccgacgatc gcggtgccgt cgtcgcagca cgaagcgctc gcggcgtggg cgaaggtcat
     2221 cgactacctc gaggagcaac ggaagatctc gctgcgcggc tattacgagt ttgcccgcgt
     2281 gctcaaatgg accgcgacgg acctcgagct cgggtttgcg gccgacgacg acagcaaatg
     2341 ggccggcgag aatgccggcg agcaagcgaa tatcaacgag ctacgcagcg tgcttgccga
     2401 tctcggccac aaggtcaagg tgacggtaag gatgctcgac gcgacggaat ctcaagccac
     2461 gcaggtgcgc tcgatcgtcg agtcgactcg agagaagtcg tcggcggagc gcacgaagcg
     2521 cgagcaggaa gcgcgcgagc atccgatcac gaagcacgtg ttgcaggcgt ttggcgcctc
     2581 catcaaggag atcaagaccg atgtctaacg gaaagatgcc cgacctcaat gctctgatga
     2641 agcaggcgca gaagctccaa ggcgatgtgc agaaagtcca agaggagctc gcgcagatgc
     2701 agtgtgatgg cgccgccggc ggcgggctcg tcaccgcgac cgtgaacggt cagttcgaag
     2761 tcgtgcgcgt gaagatcgac aagagcgtcg tcgatccgaa cgacgtcggc atgctcgagg
     2821 acttgatcac ggcggcgatc aacgcagcgg ggacgcgcgt gcgcgaagtc agcaaagaga
     2881 agatggcgtc ggtcatgggc ggcatgggcg ggatgccggg gatgccgggt ctgttctaga
     2941 tggctgccga tccgatccgc cggctggtgc aggagctgtc gcgcctgcca gggattgggg
     3001 agaagacggc gactcggctc gcgttccacc tggtgcgcgg caatcgccag caggtgctcg
     3061 acctcgcgca ggcgctcgtc gatgtcaccg acaagatccg gctgtgctcg gtctgcatga
     3121 acatgaccga ggcggatccg tgcacgcttt gcgcggaccc gcgtcgcgac ggcgaggtga
     3181 tctgcgtggt ggcgacgccc tccgacctga tcgcgatcga tcgcggcggt cattttcgcg
     3241 gccggtacca cgtgctgcac ggcctgttgt cgccgctcga gggcatcggc cctgacgaca
     3301 tccgcgtcgc cgagctcgtg cgccgcctcg gtggggaccc gccggttcgc gaggtcatca
     3361 tcgcgaccag ccccagcgtc gacggcgaag cgaccgcgat gtacgtcgcg cgcgtgctca
     3421 agcccctcgg ggttaaggtc tcgcggattg cgaccgggct gcctgtaggt ggggagctcg
     3481 aatacagcga ccaagccacg attgcccgcg ccctggccgg gcgggctcag atgtagatct
     3541 gggccccgta gagttgaacg aaatcattgg gcttgttacc gtgactcgac ggcggggagt
     3601 cctcggtttc gaagggctta cctaccagag gagccaaggc cgcgatgatc caacagctcg
     3661 tcatgtacga ggaggaactg gcgcagatca atcatgtctg cgaagtcctt catcgagacg
     3721 caaacgctag ggcggtgctc gtcatcgaca agaacggtca ggcattcgca caggcgggtg
     3781 aggtcgagca actcgacgtc acgtcactgt cgtcgctgac tgcgggtaac gtcgccgcca
     3841 ccggcgcgat cgcctcgctg ctggcggaga aggaattcgc cggtcagttc cacgaaggcg
     3901 aaaagaccaa cgtccacatc tcgatcgtgg gccagcgcgt cattctcgtc gtgctgttcg
     3961 acgagcggtc gtcgctgggt ctggtccgct tgcgcgttcg caaggcgacg ggcgagctcg
     4021 ttcaggtcct cgacgttctc acgaagaaga gccagtcggg atcgcagcca tcggtgttct
     4081 cggagatcac ggacgacgac atcgacaacc tcttcaacga ctaggccctc gcgatgagct
     4141 tcatcaatta cagttcgcgc gagatcaact gcaagatcgt ctactacggt cccggcttgt
     4201 gcgggaagac cacgaatctg cagtacatct acaacaagac gaaccctgaa gcgaaaggca
     4261 agatgatctc gctcgcgacc gagaccgagc gcaccttgtt cttcgacttc ttgccgctct
     4321 cgctcggcga gatccgcggc ttcaagaccc gcttccacct gtacacggtg ccgggccagg
     4381 tgttctacga cgcgagccgc aagctgatcc tcaagggcgt cgatggtgtc gtgtacgtcg
     4441 gcgactcgca gattgagcgc atggaagcga acatcgagtc gctcgagaac ctgcgctcca
     4501 acctgcagga gcagggctac gatctcgaca agcttcccta cgtcgtgcag tacaacaagc
     4561 gcgacctgcc caatgcgatc ccgctgccgg agctcgagga agcgttgaac ccgaccaagg
     4621 ttcc
//
LOCUS       DASUFX010000230         6075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_590109,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000230 DASUFX010000000
VERSION     DASUFX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590109"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFV99_09030"
     CDS             <1..759
                     /locus_tag="VFV99_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5059490.1"
                     /translation="TLADQKLVSKAQVEQQQAQAATDEATVSAAESNVVAQEANVRRL
                     VELQGFAKVSAPFAGTITARTIDRGTLVGEGGATPMFTLVATDPVRVFIDVPQSVAPS
                     VKVGTDAAVTVREYAGRAFAGKVTRAAGALDPELHVMSTEIQVPNTDNALLPGMYVQV
                     ALTLAVPHRVLEIPSTALYSDAQGLRVATVDAQQRIKFAPITIERDTGQSLWIATGLT
                     GDERILKIAVPTLLDGDAVEIATPPPSPPAEAKK"
     gene            complement(1018..1689)
                     /locus_tag="VFV99_09035"
     CDS             complement(1018..1689)
                     /locus_tag="VFV99_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX5059491.1"
                     /translation="MRGELHYTLTNVDGAITAVGAARACDLAPRFNIAPGQHAPVTIV
                     SDGTRVIELMRWGLLPRWRGHGGKRGPLVNAAPLEAVAGTPLLRDAFKKQRVLVLADG
                     CYAWRELKQPVCFHPEPRTVIAFAGIWNINDDDGIASFALLLGPPLVTRVNDAMPIVV
                     PPTAFEAWLDPTVSPDAATDLLAAAEHPTGWPTDTVTTRMASAQYDDAQCIAPLGNPN
                     QGELF"
     gene            1808..2791
                     /locus_tag="VFV99_09040"
     CDS             1808..2791
                     /locus_tag="VFV99_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX5059492.1"
                     /translation="MSASRREFLRDTTIALALLPQVANAKPSSKKTAVELPAVIATWD
                     YGITLCTAAQKVLAGGADILDALEKGVNAVEDDPSVHTVGYNSWPNEEGVVQLDASIM
                     DGRGHRSGAVGALEGIKNPISVARKVMDKTTHVMVVGPGALAFAKKMGFKEEPLITPE
                     TLKEWKALKKESFWRDNHDTVCCIGIDAKGNLASAVSTSGLSNKLPGRLGDSPIIGAG
                     NYCDNEIGAACATGIGELAIKNAASFAIVERMRAGLDPKRACDEILARVVAHHPEVKT
                     NDKAQLAFIAMNKRGEVGGAALRKQTKVFRYALFRGTKNAPALVDVKPLLG"
     gene            3555..4148
                     /locus_tag="VFV99_09045"
     CDS             3555..4148
                     /locus_tag="VFV99_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX5059493.1"
                     /translation="MAALDTAAESSVDAIKLDRMPWAWSARLIQRAESRNLTLLATAL
                     DEDAVTRLDWLGAAGFYLVFDWSDLDLVATAARTGKPVVLQVGTASELELAEVVSTVH
                     ANGDGGIALVQSVIDIDLEGLEALRRVGSVVGIADRSRGPAIALAAISKGASIVEKRF
                     SLRSDGKELCPAELSTVVRDCEQAWAQLGHDRRWTIN"
     gene            4153..4392
                     /locus_tag="VFV99_09050"
     CDS             4153..4392
                     /locus_tag="VFV99_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059494.1"
                     /translation="MNHPRMTSSGRRLAFFIAVLIAFALPKRVECSFPGDECAHTVGR
                     EQCTHYEVEPWVFYGLEHLFRRDIGFAYTSGEDCR"
     gene            4552..5568
                     /locus_tag="VFV99_09055"
     CDS             4552..5568
                     /locus_tag="VFV99_09055"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5059495.1"
                     /translation="MTHAQRHERELLGKATRDARHRAHGLVERVKHRPSPDAPDQVIE
                     SRVRACLGRAVSHASALDVVVVGGRVILRGPVLAHEADFTVRAVHRIPGVQHVVDQME
                     RHDKPGTIPALQGEGRAQRRAWSPTAQVAATAAGSLLFLYGMLLRRGLVGKLLGAGGA
                     ALAVRGTVNRPMAEFVGRRGGIAVQKTIVVQRPVDAVFELMSKLENFPLFMEHVKEID
                     LELGGRKSRWTVDGPAGTTIQFEAETTEYQPNKLIAWKTLPNQPVEHEGRVRFEETPA
                     GTRVHVQMTYRPPGGVVGHAIAHILGWDPKGRMDDDLVRVKALLEEGKTRAHQQRVEL
                     ADLH"
     gene            5581..>6075
                     /locus_tag="VFV99_09060"
     CDS             5581..>6075
                     /locus_tag="VFV99_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059496.1"
                     /translation="MERDIAGWLATQVDLRETAPSTTIVPKGDEVARSLALLQQMAGQ
                     SLLASVQLRPGNVIGEGGMGVVREAEQVALGRIVAIKTLKPGKRDVVAARDLLREAWV
                     SGSLEHPNVVPVHHLGLDDDGMPVLVLKRVSGVEWSKLMGDQAEVARRFGATDLLAWN
                     LGILA"
BASE COUNT         1085 a         1867 c         2076 g         1047 t
ORIGIN      
        1 acgctcgccg accagaagct cgtctcgaag gcacaggtcg agcagcagca ggcgcaagcc
       61 gcgaccgacg aagcgacggt ctcggcagcc gagtcgaacg tggtcgcgca ggaagcgaac
      121 gtgcgccggc tcgtcgagct ccaagggttt gcgaaggtca gcgcaccatt cgccggcacg
      181 atcaccgcgc gcacgatcga tcgcggcacg ctggtcggcg agggcggtgc gacaccgatg
      241 ttcacgctcg tcgcgaccga tccggtgcgc gtcttcatcg acgtcccaca gagcgtggcg
      301 cccagcgtga aggtgggcac cgatgcagcg gtgacggtgc gcgagtacgc gggccgcgcg
      361 ttcgccggca aggtcacgcg cgcggcaggc gcgctcgatc ccgagctgca cgtgatgtcg
      421 accgagatcc aggtgccgaa caccgacaac gcgctcctgc ccggcatgta cgtgcaggtc
      481 gcgctgacgc tcgcggtacc gcatcgcgtg ctcgagattc cgtcgacggc actgtacagc
      541 gacgcgcaag gcctccgcgt ggcgaccgtc gatgcgcagc agcggatcaa gttcgcgccg
      601 atcacgatcg agcgcgacac cggtcagtcg ctgtggatcg cgaccggcct caccggcgac
      661 gagcgcatcc tcaagatcgc agtgccgacg ctcctcgacg gcgatgcggt cgagatcgcg
      721 acgccgccgc cgtctccgcc ggccgaagcc aagaagtagc tcgaacgagc cttctgtaga
      781 gcatggcgtc gaaggagcgg cctgccgctt cgcggctccc agaggaacgc caaggggcca
      841 aaacgccaag aacgccaagg gaagggtttc gggatcgcgt tctgctcgtg cggtcgttcg
      901 tgcagagcgc gatcaagaag actttcttct tggcgttctt ggctgccttg gctcgtcttg
      961 gcgtttatct ccgacgcggc gaagccgcct gcgtgcgatc gtttgatctg aatcgggtca
     1021 gaagagctcg ccctggttcg gattgccgag tggcgcgatg cactgcgcgt cgtcgtactg
     1081 cgccgacgcc atgcgcgtcg tcaccgtgtc ggtgggccag cccgtcgggt gctcggcagc
     1141 ggcgagtagg tcggtcgcag cgtcggggct gacggtcgga tcgagccaag cttcgaacgc
     1201 ggttggcggc acgacgatcg gcattgcatc gttcacacgc gtgacgagcg gtgggccgag
     1261 cagcagcgcg aagctcgcga taccgtcgtc gtcgttgatg ttccagatgc cggcgaacgc
     1321 gatcaccgtg cgtggctcgg ggtggaagca caccggctgc ttgagctcgc gccacgcgta
     1381 gcagccgtct gcgagcacga gcacgcgttg tttcttgaac gcgtcgcgga gcagaggcgt
     1441 gccggcgacg gcttcgagcg gcgccgcatt gacgagcggt ccccgtttgc cgccgtgacc
     1501 acgccagcgc ggcaacagcc cccaccgcat gagctcgatg acgcgggtgc cgtcgctcac
     1561 gatcgtaacg ggcgcatgct gcccgggggc aatgttgaaa cgtggcgcca gatcacaggc
     1621 gcgcgcggca ccgactgccg taatcgcgcc gtcgacgttg gtcagcgtgt aatgcagctc
     1681 gccacgcatc gcgttccctc tcgcccaagc gtagctagaa tcgcgggact gtgaagtaaa
     1741 gctggcgtca acgacggcaa gtgcgacagt ggcgctcaca gcgcggcgcg ggtgtaaggt
     1801 cccccgcatg tcagccagcc gccgtgaatt cctgcgtgac acgaccattg cgttggccct
     1861 gctcccacag gtcgcaaatg cgaagccgtc gtcgaagaaa accgctgtcg agctcccggc
     1921 ggtgatcgcc acctgggact acggcatcac gctgtgcacc gctgcacaga aggtgctcgc
     1981 cggcggcgcc gacatcctcg acgcgctcga gaagggcgtg aacgcggtcg aggacgatcc
     2041 gtccgtgcac acggttggct acaactcgtg gccaaacgaa gagggtgtcg ttcagctcga
     2101 cgcgtcgatc atggatggcc gcggtcatcg cagcggcgcg gtcggtgcgc tcgaaggcat
     2161 caagaatccg atctcggtcg cgcgcaaggt gatggacaag acgacgcacg tgatggtcgt
     2221 cggcccgggt gcgctcgcgt tcgcgaagaa gatgggcttc aaggaagagc cgctgatcac
     2281 accggagacg ctgaaggaat ggaaggcgct gaagaaggag agcttctggc gcgacaacca
     2341 cgacaccgtg tgctgtatcg gcatcgacgc gaaaggcaat ctcgcgagcg cggtgtcgac
     2401 tagcggactc tcgaacaaac tacctggccg actcggcgac tcgccgatca tcggcgcggg
     2461 caactactgc gataacgaga tcggtgcggc gtgcgcgact ggcatcggcg agctcgcgat
     2521 caagaacgcg gcgtcgttcg cgatcgtcga acgcatgcgc gcgggtctcg atccgaaacg
     2581 cgcatgcgac gagatcctcg cgcgcgttgt cgctcaccat cccgaggtga agacgaacga
     2641 caaagcgcag ctcgcgttca tcgcgatgaa caagcgcggc gaggtcggcg gtgccgcgct
     2701 ccgcaagcag accaaggtgt ttcggtacgc actgttccgt gggacgaaaa atgcgcccgc
     2761 gctcgtcgac gtgaagccac tgctgggctg aagacaaaca tcggatgagg tgcgtttctc
     2821 gtcgggaatc acccatccgg gtactgcagc ctacgtggtg tgtcagaccg ctttggtatg
     2881 acagctcaca tggagcgatt ccctcgagga agcctggcgc catatgactt ccctgcggtt
     2941 gccacggtag cgcccacagc agtgagcaag ccgcctacgg tcatcagcgc agaaacagcc
     3001 gccacggatg cagaagctgc ggcgaagcaa tgtgatctct ccacgacatg ccccaattgc
     3061 agcgcggaga tggttcccga gcacgcgcac taccgctgtg gagcatgcgg ctacagagat
     3121 tcgtgttgct tctgacagtc gcgaacagcg gagttgcgat cactaccgtc gatcgtgatt
     3181 cgtgtgtgca ctgacgttcg acgagcaaat cgcaattcga attgcttctg atcgcggatc
     3241 gcgattcgcg ttcgtgccag ctcactgtta gcgaaaagga cccgttcgct aacccgcaca
     3301 cgctcgacgt tgtgcgagag tgttcttcgt cgacggcttt ggggagtcgc tcgacgagtt
     3361 cgcgaaacga gttcttctga gcgatggctc ggtagagaca tctcaccgac catccacgtc
     3421 gatgtcggtc atgctttgac gactcggggg gagtaatgag accgatggat tttggacgac
     3481 cggtgggggc cgctcatccg ccattcatta tcgctgcact ggattgtcga gagctgggaa
     3541 ctctcgatcg cgccatggca gcactcgaca ctgcagctga aagcagtgtc gacgcgatca
     3601 agctcgatcg catgccgtgg gcatggagcg cgcgactgat ccagcgcgcc gagtcgcgta
     3661 acctgacgct gctcgcgact gctctcgacg aagatgcagt gacgcgactc gattggctcg
     3721 gtgcagccgg cttctatctc gtgttcgatt ggtccgatct ggatctcgtc gcgacggccg
     3781 cgcgcacggg taagccggtc gtgctgcagg tcggcaccgc atccgagctc gagcttgccg
     3841 aagtcgtctc gaccgttcat gcgaacggcg acggcggcat cgcgctcgtg caatcggtga
     3901 tcgacatcga cctcgaaggt ctcgaagcgc tccgccgggt cggctcggtg gtcggcatcg
     3961 cggatcgttc gcgtggtccg gcgattgcgc tcgccgcgat ctcgaagggt gcgagcatcg
     4021 tcgagaaacg gttctcgctg cgcagtgacg gtaaggagct atgcccggcc gagctctcga
     4081 cggtcgttcg cgactgcgag caggcatggg cacagcttgg tcacgatcgc cgttggacga
     4141 taaactagtc gcgtgaatca cccgcggatg acgtcgagtg gacgccggct ggcgttcttt
     4201 atcgccgtcc tgatcgcatt cgcattaccg aagcgcgtcg agtgcagctt tccgggcgac
     4261 gagtgcgcgc acacggtcgg ccgcgagcaa tgcacgcact acgaagtcga gccgtgggtg
     4321 ttttacgggc tcgagcactt gttcagacgc gacatcggct ttgcgtacac gtcgggcgaa
     4381 gactgtcgct aagcgattgt cgctctggcg cgcagcttgc gacgcctcac cttcggaggc
     4441 gcagcaatga aggtagcgaa tttccccggt gccgtattac tcggtgctgg gctcatgttt
     4501 tttctcgatc cggtgcgcgg acgcaaacgg cgcgcgcgga tgaacgaagc gatgacgcac
     4561 gcgcaaaggc acgagcgcga gcttctcggc aaagcgacgc gtgatgctcg gcatcgtgcg
     4621 catggtttgg tcgaacgcgt aaagcatcgg ccatcgcctg atgcgccaga tcaggtgatc
     4681 gagagccgcg tgcgggcgtg tctcgggcgc gccgtgtcgc atgcgagcgc gttagacgtt
     4741 gtcgtggtcg gcggccgcgt gatcttgcgc ggtccggtac tcgctcacga ggcggacttc
     4801 acggtgcgcg cagtacatcg cattccgggc gttcagcacg tcgtcgatca aatggagcgt
     4861 cacgacaagc cgggcaccat cccagcgctg caaggggagg gacgtgcgca gcgtcgtgcg
     4921 tggtctccga cggcgcaggt ggcggcgacg gcagcgggct cgctgctgtt tctgtacggc
     4981 atgctcctgc gcagaggcct cgtcggcaag ctcctcggcg ccggcggcgc tgcgcttgcg
     5041 gtgcgtggca ccgtcaatcg accgatggcc gagttcgtcg gccgccgcgg tggcatcgcg
     5101 gtgcagaaga cgatcgtggt gcagcgtccc gtcgacgcgg tgttcgagct gatgagcaag
     5161 ctcgagaact tcccgctgtt catggagcac gtgaaggaga tcgatctcga gctcggcgga
     5221 cgcaagtcac ggtggactgt cgacggtcca gctggcacga ccatccagtt cgaggcggag
     5281 accaccgagt accagccgaa caagctgatc gcgtggaaga cgctgccgaa tcaaccggtc
     5341 gagcacgaag gccgcgtgcg gttcgaggag acgccggcgg gcacgcgcgt gcacgtgcag
     5401 atgacctatc gcccgccggg cggcgtcgtc ggtcacgcga tcgcgcatat cctcggctgg
     5461 gacccgaagg ggcgcatgga cgacgacctc gtgcgcgtga aagcgttgct cgaagaaggc
     5521 aagacgcgcg cgcaccagca acgcgtcgag ctggccgatc tgcactgata tagtcggcct
     5581 gtggagcgag atatcgcagg gtggcttgcg acgcaggtcg atctgcgcga gacggcgcct
     5641 agtacgacga tcgtgccgaa gggcgacgag gttgcgcgct cgctcgcgct gctccagcag
     5701 atggcgggcc agtcgttgct cgcctccgtg cagctgcggc cgggcaacgt catcggcgaa
     5761 ggcgggatgg gcgtcgtccg cgaggcggag caggtcgcgc tcggtcgcat cgtcgcgatc
     5821 aagacgctga agcccggcaa gcgcgacgtg gtcgcggcgc gcgacctgtt gcgcgaagca
     5881 tgggtgagcg gctcgctcga gcacccgaac gtcgtgccgg tccatcatct cggcctcgac
     5941 gatgacggca tgccggtgct cgtgctcaag cgcgtctccg gtgtcgagtg gagcaagctg
     6001 atgggtgacc aggccgaggt cgcgcggcgg ttcggcgcga ccgatttgct cgcgtggaac
     6061 ctcggcattc tcgcg
//
LOCUS       DASUFX010000231         9400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1008387, whole genome shotgun sequence.
ACCESSION   DASUFX010000231 DASUFX010000000
VERSION     DASUFX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008387"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFV99_09065"
     CDS             <1..652
                     /locus_tag="VFV99_09065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5059497.1"
                     /translation="ALRAFVAAGGKQLVLLGAGFDCRALRLPELADVRVLEVDHPATQ
                     ARKRSVLDQIGAHSHATYVAWDFEGRAMEDLPDILDEAGLEVALPVFTIWEGVTMYLT
                     EPAIDSSLRAIRAFGGPGSQLALTYFAKSRIEKPSLITRAMAAVVATFGEPWKFGWTP
                     EELPDYLADRGFSLMRDVAMSEAARELLPPDLAAHVANPDRRFAVAASESIAVAAS"
     gene            756..1295
                     /locus_tag="VFV99_09070"
     CDS             756..1295
                     /locus_tag="VFV99_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059498.1"
                     /translation="MQRILLPVVAALGLAAGCYTQGDVGVGYSYGYAAPAPSMYYVEP
                     GVSVVAYSDYPVFYSDNYYWRYDGGLWYRSSYYNGGWVAWNNVPYRVRSIDRPARYAR
                     FTPGQGWSREPYNGGYRGSYQGGVRDHRTYQPAYRSTYQAPAPNVRDHRAPTYSAPQP
                     QPRTVAPGGGPAVRDHRRR"
     gene            complement(1350..1805)
                     /locus_tag="VFV99_09075"
     CDS             complement(1350..1805)
                     /locus_tag="VFV99_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5059499.1"
                     /translation="MSATRITLYINAPRSDIYRAFLDAKAVATWMVPTGMSSHVHVFE
                     PRIGGRFRISLTYDAPTDTGKTSPQTDTFHGRFVELVPDQRVVELIEFETDDPTMKGE
                     MTVTIELADADEGTQLTAVHDNVPPGISPADNELGWSISLRKLATLVES"
     gene            complement(1802..2752)
                     /locus_tag="VFV99_09080"
     CDS             complement(1802..2752)
                     /locus_tag="VFV99_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059500.1"
                     /translation="MTSYVHLRRYGKKPSGSEVATVDVIADGQVVYRGHRYDLRTGEK
                     GAKQAGFAHCTDGRYLQWNQTESGLEIHRGDGRVATAPVVGGKLIALVGPYLVWADDA
                     RQPWMVIDAGTGELAGRVEDQRGDATHGTVLYSPQSFDPKSGNTLWLCEGSRLAELDV
                     AKRRMARTIEADAEHVFIGVAALSDGHVITLARTLEDKAKHNRSADRIVLFSPTGERV
                     RDVPGDVMHLNRIGDQFIASDDRKEQFVIYDRSLEPVARVEMFEPGRDAYNMVVPLPS
                     GREWIGVGGRGEWDHYGEPDLVPVKPKKPAKPTAKKAKPA"
     gene            complement(2779..6357)
                     /locus_tag="VFV99_09085"
     CDS             complement(2779..6357)
                     /locus_tag="VFV99_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059501.1"
                     /translation="MLLPLALTSCRSPAPKTTTTTVGAGGGGSASMPPVTVDATLDAL
                     TEGAKVRGFTVQALYLDDADKPMGARFVHDATGFTFDYLRIETAPQGYIWVNSFPTSD
                     MGEPHTQEHLLLGKGDRGRKLGSSESMSLAESSAFTAQWRTAYHFHTVAGQDVFWPVF
                     ENHLDALLNPDYTDEEIRREVRNFGVDKADDGTLRLEEKGTVYNEMVRTFESPETQAW
                     ETAGRLVYGPKHPLAMTSGGAPDAIRDMTPKDIRTFHTANYHLANMGMIGAFPSSMSL
                     ASVLDQTAAILVKESGRKGKVTTEADLPKPSAAAAGTIKVVDYPYASTTNPGPMMLVW
                     PAARNLDQTEHTLLGLFLDAFAGDEGTPLYKKLIDGKTRVMDLGASGVWSFTSNDQGQ
                     PVYVGLSGVKSDKLDEKTIGDVRTLVLGELDRIAKLPDGSPELVALTERLQSRVIDLR
                     RRLNKFLDTPPGFGLRSASDAWSSHLQELSKVKGFKKSLTFRPELAAIEQILAAKGNP
                     FRDRLRTWGLLESPFGVAARPSPTERTRLDKERDARVTAELARLQKQYATKDAAATLG
                     AYQKDYDAQTKQLEDSQKSTELPPLVDTPPMTLDDGLIYTSTAIGNVPAFVATFDSMV
                     SARVQVAFNIRNRVKPADQMFLAGLPMLMSDAGVIVDGKPIAADEMRERMRKEILELS
                     VYYVDNPKTKRAELVIAGAGNGAAETKQAIVWMRRVLFAPDWRIENLPRLRDLVDQSL
                     TSLRQRTLGAEEGWVDDPRDAWWEQSALHAHIGTFLTRTHDLHRLRWMLSDTGDKKAN
                     DEAMKFLASLADAKSLKRAELMELSEALAKGTKPKTATLARWTDAAGKLSEKAKPLAI
                     QAGKHLGALVPDLPDGSLAGDWNYLCREMAADLKVGAPAALVKLEEVRKAVVQGTRAR
                     IVEVGSKANQAALAGDVEAFVREVPAETVADVADSTDKPFRARLAQRDPKAKSPQFVG
                     LVVPSTSSGVFLNLAPNAYYTDADEGHVLDYLTGNLYSGHGAHSIFMKTWAAGLAYSN
                     GLRPSISDGTIRYYAERCPLLPQTLRFVIDQLKKAKSDPNIARYAIAKAFDSRVAIGF
                     EARASAMAANLVDGLTPEVVKTFRSRVLDATKRPDLANELFGRMEAVYGKVLPGYAKL
                     DPNAIYFVIGPEKQIAAYQDYLKSAVGKDATLHRLYPRDFWIPAKL"
     gene            6475..7335
                     /locus_tag="VFV99_09090"
     CDS             6475..7335
                     /locus_tag="VFV99_09090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5059502.1"
                     /translation="MRRWLIVLALASSADAFAQPVTNALPDTGDPAVIALLNASDQLV
                     CTAAVIAPHTVLTAAHCLAGKPLTLQAFFGSSVEDGGTKIAVTDARQHPQFDPGGNDI
                     GVITLADPAPVTPLPLAPPIDGSIVDTTFRVVGFGTTGAAGGEGVKRLGTAKVAGISA
                     EDFIAVPDLSLSCLGDSGGPALFTGDTIAGVVSRVDGQCVDHAVYTRVDIAMDVLIQP
                     YLDETAPGAADEGEPCFYDGHCASGLECVGDTTRTCESTGCGCRTSKPSGLWLIVLAL
                     LIRRRNQNGV"
     gene            complement(7353..8366)
                     /locus_tag="VFV99_09095"
     CDS             complement(7353..8366)
                     /locus_tag="VFV99_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5059503.1"
                     /translation="MATLTERWLRPRTPRTQFPPYEYGDPTIVGLAEARTSTVARIYH
                     KGHEQAWDGRAVLDELVAKHGGINFPEDKKEAFGRVASVLLWGELAAWSISADLALKL
                     EDTPAKMAASSQVFDEARHFYVLRDYMWRAGIPITKLGGWSRRLLVELLETENLMHKV
                     VGMQLLVESTAVVMFRMIAEAKIEPVLSELLYYFERDEARHVGLGVLTLPEVLAGLSE
                     PDAWRLWWFQTRMNLGMTMGGITLRHAFDTLGIDQAEMNMQGFKYHTEILRRMRKNEP
                     GKGADAKSVRGLFKISRKGQDAFQEFFFPTKPRSPRAAKMFDAIVTFAEKSDAWLRAR
                     SPL"
     gene            complement(8395..9018)
                     /locus_tag="VFV99_09100"
     CDS             complement(8395..9018)
                     /locus_tag="VFV99_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5059504.1"
                     /translation="MRSEARQRQILSVAKKVFAERGFHAANVSHICEAAGIGRGTLYQ
                     YFANKHSVLTAILRETLDRVRALMETMSRDAAYPPAESVTKAVAIKVSISQLRRLLGV
                     VFDDVDTLRILLREAVGLDAQVETILGEIDDTLIRILEQDLIRAQKAGYIRECDPHIV
                     ATMIVGGVEKLGLAALRSSAPVDLDALAREAATMHAIGLMSDRLKPE"
     gene            9163..>9400
                     /locus_tag="VFV99_09105"
     CDS             9163..>9400
                     /locus_tag="VFV99_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5059505.1"
                     /translation="MDLDQTIKAHLASGELAVAATVALGGLGPQILGYLAAQLRDDDA
                     AYDVFGHFCEELWKSIGNFRGDSSFKTWAYKLVMH"
BASE COUNT         1463 a         3144 c         3054 g         1739 t
ORIGIN      
        1 cgcgctccgt gcatttgtcg ccgccggagg caaacagctc gtgctgctcg gcgccggctt
       61 cgattgccgc gcgctgcgcc tgcccgagct agccgatgtg cgcgtgctcg aggtcgatca
      121 tccggcgacg caagcgcgca agcgttcggt gctcgatcag attggcgcgc actcgcacgc
      181 gacgtacgtg gcgtgggact tcgagggccg cgcgatggag gacttgccgg acatcctcga
      241 cgaggcaggc ctcgaggtcg cgctgcccgt gttcacgatc tgggaaggcg tgacgatgta
      301 cctgacggaa ccggcgatcg actcgtcgct gcgtgcgatt cgtgcgttcg gcggaccggg
      361 ctcgcagctc gcgctcacgt acttcgcgaa gtcgcgcatc gagaagccgt cgctgatcac
      421 gcgcgcgatg gcggcggtcg tcgcgacgtt cggcgagccg tggaagttcg gttggacccc
      481 ggaagagctg cccgactatt tggccgatcg cggcttctcg ctcatgcgcg acgtcgcgat
      541 gtcggaggcc gcgcgcgagc tgttgccgcc ggatctcgcg gcgcatgtgg cgaatccaga
      601 ccgacgtttc gccgtggcgg catcggaatc gattgccgtt gccgcgtcgt aactttgtgt
      661 tgaagcgtgt acgtgagcca gagcacgacg agggtttcag tacttagacc tgtcaagtcg
      721 tgcattcacc gacggtgaga gctaggatct ggttcatgca gcgaatcctt ttgcccgtgg
      781 tagcagcgct cggcctcgcg gccggttgtt acacccaagg cgacgttgga gtcggctact
      841 cgtatgggta cgcggcgcca gcgcccagca tgtattacgt agagcccggc gtctccgtcg
      901 tggcgtactc ggattatccg gtcttctact cggataacta ctattggcgc tacgacggcg
      961 gcctctggta ccgctcgagc tactacaacg gcggctgggt cgcgtggaac aacgtgccgt
     1021 accgcgtgcg ctcgatcgat cgcccggctc gctacgcgcg gttcacgccg ggccaaggct
     1081 ggtcgcgcga gccgtacaac ggcggctatc gtggcagcta ccaaggcggc gttcgcgatc
     1141 accgcacgta ccagcccgcg tatcgcagca cgtaccaggc gcccgcgccg aacgtccgcg
     1201 atcatcgcgc gccgacctac tcggctccgc agccgcagcc ccgtacagtt gcgcccggcg
     1261 gtggacccgc ggtccgcgac catcgtcgtc gctgatcgca gctaactgca cgtagagcgc
     1321 cggcctcgtg ccggcgtttt cgttttcggt cagctctcga cgagcgtcgc gagcttgcgc
     1381 agcgagatgc tccagccgag ctcgttgtcc gcaggcgaga tgccgggcgg aacattgtcg
     1441 tggaccgcgg tgagctgcgt gccctcgtcc gcgtcggcga gctcgatggt caccgtcatc
     1501 tcgcccttca tcgtcgggtc atcggtctcg aactcgatca gctcgacgac gcgctggtcg
     1561 ggcaccagct cgacgaagcg gccgtggaac gtgtcggtct gcggcgacgt cttgccggtg
     1621 tcggtcggcg cgtcgtacgt gagcgagatg cggaagcggc cgccaatacg cggctcgaac
     1681 acgtgcacgt ggctggacat gccggtcggc accatccacg tcgcgaccgc ttttgcatcg
     1741 agaaatgcgc gatagatgtc ggagcgtggt gcgttgatgt acagcgtgat gcgcgtggcg
     1801 ctcacgccgg cttcgctttc ttcgcggtgg gcttcgccgg cttcttcggt ttgaccggca
     1861 cgagatcggg ctcgccgtaa tgatcccact cgccgcggcc gccaacgccg atccattcgc
     1921 gaccgctcgg caacggtacg accatgttgt acgcgtcgcg gccgggctcg aacatctcga
     1981 cgcgtgcgac cggctcgagc gaccggtcgt agatgacgaa ctgttccttg cgatcgtccg
     2041 acgcgatgaa ctggtcgccg atccgattca ggtgcatgac gtcacccggg acatcgcgta
     2101 cgcgctcgcc cgtcggcgag aacaggacga tgcgatcggc gctccgattg tgcttcgcct
     2161 tgtcctcgag cgtgcgcgcg agcgtgatca cgtgaccatc cgacagcgcg gcgacaccga
     2221 tgaacacgtg ctcggcatcg gcctcgatcg tgcgcgccat ccggcgcttt gcgacatcga
     2281 gctcggcaag ccgcgagcct tcgcacagcc acaatgtgtt gccgcttttc ggatcgaacg
     2341 actgcggcga gtacagcacc gtgccgtgcg tcgcatcgcc gcgctgatcc tcgacacggc
     2401 cagcgagctc accggtgccg gcgtcgatca ccatccacgg ctgccgcgcg tcgtccgccc
     2461 acacgaggta cgggccgacg agcgcgatca gcttgccgcc gacgacaggt gccgtcgcga
     2521 cgcggccatc gccgcgatgg atctcgaggc cgctctcggt ctggttccac tgcaagtagc
     2581 ggccgtcggt gcagtgcgcg aagcccgcct gtttagcgcc cttctcaccg gtgcgcagat
     2641 cgtagcggtg gccgcgatac acgacctgcc cgtccgcgat gacgtcgacc gtcgcaacct
     2701 cggagccgct cggcttctta ccgtagcggc ggagatggac gtaactcgtc atcgttcgag
     2761 gatacaggag aacgctggct acagttttgc agggatccag aagtcgcgtg gatacaagcg
     2821 atgcaacgtc gcatccttgc ccactgcact tttcaagtag tcctggtaag cggcgatctg
     2881 cttctccggt ccgatcacga agtagatcgc gttcggatcg agcttcgcgt acccgggcaa
     2941 caccttgccg tagacggcct ccatgcggcc gaacagctcg ttcgcgagat ccggtcgctt
     3001 cgtcgcgtcg agcacgcgcg accggaacgt cttcacgacc tcgggcgtga ggccatcgac
     3061 gaggttcgcg gccatcgcgc tcgcgcgggc ctcgaagcca atcgcgacgc gcgaatcgaa
     3121 tgccttcgcg atcgcatagc gcgcgatatt cggatcggat ttcgctttct tcagctgatc
     3181 gatcacgaag cgcagcgtct gcggcaatag cgggcagcgc tccgcgtagt accggatcgt
     3241 gccgtcactg atgctcgggc gcaggccgtt cgaatacgcg aggccggccg cccaggtctt
     3301 catgaagatc gagtgcgcgc cgtggccgga gtagaggttg ccggtcaggt agtcgagcac
     3361 gtggccttcg tcggcgtcgg tgtaatacgc gttcggcgcg aggttcaaga acacgccgct
     3421 cgacgtcgac ggcacgacga gtccgacgaa ctgcggcgac tttgctttcg gatcgcgctg
     3481 tgcgagacgc gcgcggaacg gcttgtcggt gctgtcggcg acgtcggcga ccgtctcggc
     3541 cggcacctcg cgtacgaacg cttcgacatc gccggcaagc gccgcctggt tcgctttcga
     3601 accgacctcg acgatgcgag cgcgtgtgcc ctgcaccacc gccttgcgta cctcctcgag
     3661 cttcacgagc gccgcaggtg cgccgacctt gagatccgcc gccatctcgc ggcacaggta
     3721 attccagtcg ccggcgagcg agccatcggg cagatcgggg acgagcgcac cgaggtgctt
     3781 gcccgcttgg atggcgagtg gcttcgcctt ctccgaaagc ttgccggcgg cgtcggtcca
     3841 gcgcgcgagc gtcgcggtct tcggcttcgt gcccttcgcg agcgcctcgg acagctccat
     3901 cagctcggcg cgcttcagcg acttcgcatc cgcgagcgac gcgaggaact tcatggcctc
     3961 gtcgttcgct ttcttgtcgc cggtgtccga cagcatccag cgcagacgat ggaggtcatg
     4021 cgtgcgcgtc aggaacgtgc cgatgtgcgc gtgcagcgcg gattgctccc accaggcgtc
     4081 gcgcggatcg tcgacccaac cctcctcggc gccgagcgtg cgctggcgca gtgacgtaag
     4141 gctctgatcg acgaggtcgc gcaggcgcgg caggttctcg atgcgccaat cgggcgcgaa
     4201 caacacgcgg cgcatccaca cgatcgcctg cttcgtctcg gccgcgccgt tgccggcacc
     4261 tgcgatcacg agctcggcgc gcttcgtttt cggattgtcg acgtagtaga cgctcagctc
     4321 gaggatctcc ttgcgcatgc gctcgcgcat ctcgtcggcg gcaatcggct tgccgtcgac
     4381 gatcacgccc gcgtcgctca tcagcatcgg cagacccgcg aggaacatct ggtcggccgg
     4441 cttcacgcga ttgcggatgt tgaacgcgac ctgcacgcgc gcgctgacca tcgaatcgaa
     4501 cgtcgccacg aacgccggca cattgccgat cgccgtcgac gtgtagatca aaccatcgtc
     4561 gagtgtcatc gggggcgtat cgacaagcgg cggcagctcg gtcgacttct gcgagtcctc
     4621 gagctgcttc gtctgcgcgt cgtagtcctt ctggtaggcc ccgagcgtcg ccgccgcgtc
     4681 cttcgtggcg tactgcttct gcagtcgggc gagctcggcg gtaacgcggg catcgcgctc
     4741 cttgtcgagc cgcgtgcgct cggtcgggct cggccgcgcg gcgacgccga acggcgactc
     4801 gagcagcccc cacgtgcgca ggcgatcgcg gaacgggttg cccttcgcgg cgaggatctg
     4861 ctcgatcgcg gcgagctcgg ggcggaacgt gagcgacttc ttgaagccct tcaccttcga
     4921 cagctcctgg aggtggctgc tccaggcgtc actggcgctg cgaagtccga accccggcgg
     4981 tgtgtcgagg aacttgttga gccggcggcg cagatcgatc acgcggctct gcagccgctc
     5041 ggtcaatgcg accagctcgg gggagccatc gggcagcttc gcgatccggt cgagctcgcc
     5101 gagcacgagc gtccgcacgt caccgatggt cttctcgtcg agcttgtcgg acttgacgcc
     5161 cgagagcccg acgtacaccg gttggccctg atcgttcgac gtgaacgacc acacgccgct
     5221 cgcgccgaga tccatcacgc gcgtcttgcc gtcgatcagc ttcttgtaca gcggcgtccc
     5281 ctcatcgcct gcgaacgcgt cgaggaacaa gcccagcagc gtgtgctcgg tctggtcgag
     5341 gttccgcgct gccggccaca cgagcatcat cgggccggga ttcgtcgtcg acgcgtacgg
     5401 ataatcgacg accttgatcg tgcccgcggc ggcggcggac ggtttcggca gatcagcctc
     5461 cgtcgtcacc ttgcccttgc ggccgctctc cttcacgaga atcgcggcgg tctgatcgag
     5521 cacgctcgca agcgacatcg acgacgggaa cgcgccgatc atgcccatgt tcgcgaggtg
     5581 atagttcgcg gtgtggaacg tgcggatgtc cttcggcgtc atgtcgcgaa tcgcatccgg
     5641 cgcgccgccc gacgtcatcg cgagcggatg cttcgggccg tagacgagac ggcctgccgt
     5701 ctcccacgcc tgcgtctccg gcgattcgaa cgtgcgcacc atctcgttgt agacggtgcc
     5761 cttctcctcg aggcgcagcg tgccgtcgtc cgctttgtcg acgccgaagt tgcgcacctc
     5821 gcggcgaatt tcttcgtcgg tgtagtcggg gttgagcagc gcgtcgaggt ggttctcgaa
     5881 cacgggccag aacacgtctt ggccggcgac cgtatggaag tgataggcgg tacgccactg
     5941 cgcggtgaac gcgctcgact cggcgagtga catcgactcg ctgctgccga gcttgcggcc
     6001 gcggtcgccc ttgccgagca acaagtgctc ctgcgtgtgc ggctcgccca tgtcgctcgt
     6061 cgggaacgag ttgacccaga tgtagccttg cggcgcggtc tcgatccgca ggtagtcgaa
     6121 cgtgaagccg gtcgcgtcgt ggacgaagcg cgcgcccatc ggcttgtcgg catcgtcgag
     6181 gtagagcgcc tgcacggtga atccacgcac cttcgcgcct tcggtgagcg cgtccagcgt
     6241 cgcgtcgacc gtcaccggtg gcatggacgc cgagccacct ccaccggcgc cgaccgtggt
     6301 cgtcgtggtc ttcggagcgg gcgaacgaca gctggtgagc gcaaggggta ggagcatggt
     6361 gatcacgtga tggcgaagca tcgccgcgca ctctagccga tcacggcccc gtcgaggttc
     6421 ccgtcacctg cgacagccca gcgtcgcacc gtgtccgtcc tgctacgatg cagcatgcgc
     6481 cgttggctga tcgtgcttgc cctcgcgtcg agtgccgacg cgttcgcgca gccggtcacg
     6541 aacgccctgc ccgataccgg cgacccagcc gtgatcgcgc tgctcaacgc cagcgatcag
     6601 ctcgtctgca ccgcggccgt gatcgcacca cataccgtcc tgaccgcggc acactgcctc
     6661 gctggcaagc cgctcacgtt gcaagcgttc ttcggcagct cggtcgagga cggcggtacg
     6721 aagatcgcgg tcaccgacgc gcgccaacac ccgcagttcg atccgggcgg caacgacatc
     6781 ggcgtgatca cgctcgccga tccggcaccg gtgacgccgc tccctctcgc gccgcccatc
     6841 gatggctcga tcgtcgatac gacgtttcgt gtcgtcggct tcggcacgac cggcgccgcc
     6901 ggcggcgaag gcgtgaagcg tctcggcacc gcgaaggtcg ccgggatcag cgcggaggac
     6961 ttcatcgcgg tgcctgacct gtcgctatcg tgtctcggtg actccggcgg ccctgccctg
     7021 ttcaccggcg atacgatcgc tggcgtcgtc agtcgcgtcg acgggcaatg cgtcgatcac
     7081 gccgtctaca cgcgcgtcga catcgcgatg gacgtcttga tccagcccta tctcgacgag
     7141 accgcgcccg gcgccgcaga tgaaggcgag ccgtgcttct acgatggcca ctgcgcgagc
     7201 ggcctcgagt gcgtcggcga caccacgcgc acatgcgaga gcaccggctg cgggtgccgt
     7261 acgagcaagc cgagtgggct gtggctcatc gtgctcgcgc tgctgattcg tcggcgcaac
     7321 caaaacgggg tctgaccggt ctgacccctt ttctacagcg gcgaacgcgc gcgcagccac
     7381 gcgtcgctct tctcggcgaa cgtcacgatc gcatcgaaca tcttcgcggc gcgcggcgac
     7441 cgcggcttcg tcgggaagaa gaactcctgg aacgcgtcct gacccttgcg cgagatcttg
     7501 aacaagccgc gtacgctctt cgcgtcggcg cccttgcccg gctcgttctt ccgcatccgc
     7561 cgtaggattt cggtgtggta cttgaagccc tgcatgttca tctcggcttg atcgatcccg
     7621 agcgtgtcga atgcgtggcg cagcgtgatg ccgcccatcg tcatcccgag gttcatgcgg
     7681 gtctgaaacc accacagccg ccacgcgtct ggctccgaca gcccggcgag cacctcgggc
     7741 agcgtgagca cgccgagacc aacgtggcgc gcttcgtcgc gctcgaagta atagagcagc
     7801 tcgctgagca cgggctcgat cttcgcctcg gcgatcattc ggaacatgac gaccgccgtg
     7861 ctctcgacga gcagctgcat gccgacaacc ttgtgcatga ggttctcggt ctcgagcagc
     7921 tcgacgagca agcgccggct ccagccgccg agcttcgtga tcggaatgcc ggcgcgccac
     7981 atgtaatcgc gcaggacgta gaagtggcgc gcttcgtcga acacctggct cgacgcggcc
     8041 atcttcgccg gcgtgtcttc gagcttcagc gcgaggtccg cgctgatcga ccacgccgcg
     8101 agctcgcccc agagcagcac gcttgcaacg cgaccgaacg cttccttctt gtcctcggga
     8161 aagttgatgc cgccgtgctt ggcgacgagc tcgtcgagca ccgcgcgacc gtcccacgcc
     8221 tgctcgtggc ccttgtgata gatgcgcgcg accgtgctcg tccgcgcttc ggcgagcccg
     8281 acgatcgtgg ggtcaccata ttcgtacggc ggaaactgcg tgcggggtgt gcggggccgt
     8341 agccagcgtt ccgtcagcgt cgccatgttg tctccttcgt agtcccatcc ctcgtcattc
     8401 gggcttcaat cgatccgaca tgaggccgat cgcgtgcatc gtggcggctt cgcgcgcgag
     8461 tgcatcgaga tcgactggcg ccgagctgcg cagtgcagcg aggccgagct tctcgacgcc
     8521 gccgacgatc atcgtcgcga cgatgtgcgg atcacattcg cggatgtaac cggccttctg
     8581 cgcgcgaatc agatcctgtt cgaggatgcg gatcaacgtg tcatcgatct cgccgaggat
     8641 cgtctcgacc tgtgcgtcga gaccgaccgc ctcgcggagc aagatgcgta gcgtgtcgac
     8701 gtcgtcgaac accacgccga gcagccgccg cagctgactg atcgacacct tgatcgcgac
     8761 cgccttcgtg acgctctcgg ccggcggata tgcggcgtcc cgcgacatcg tctccatgag
     8821 cgcgcgcacg cggtcgagcg tctcgcgcaa gatcgcggtg agcaccgagt gcttgtttgc
     8881 gaagtactgg tacagcgttc cgcgtccgat acccgccgcc tcacagatgt ggctgacgtt
     8941 cgcggcgtga aatccccgct ccgcgaacac cttcttcgcg acgctgagga tctggcgctg
     9001 ccgcgcctct gaacgcatgt agctcatcgt ttggggatcg tgccattttg gcacatcaag
     9061 tgtccggatc tattcggaac tgacgtgtca gttctagctc cgaacgcacc gcggcgcaag
     9121 ccccttcttt tctccgtcac atcacggcag tgtcggcaca ccgtggatct cgaccaaacg
     9181 atcaaggcgc acctggcaag cggcgagctt gcggtcgccg cgacggtcgc gctcggcggt
     9241 ctgggtccgc agattctcgg ctacctcgca gcgcagcttc gcgatgacga tgcggcgtat
     9301 gacgtgttcg ggcatttctg cgaggagctg tggaagagca tcggcaactt ccgcggtgac
     9361 agctcgttca agacctgggc gtacaagctc gtcatgcact
//
LOCUS       DASUFX010000232         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_34972,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000232 DASUFX010000000
VERSION     DASUFX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34972"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFV99_09110"
     CDS             <1..1180
                     /locus_tag="VFV99_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5059506.1"
                     /translation="DYDMLKGVARVENPTGQFFIQGSRFGGTPMMTKRGGKASSFQIN
                     AVHHNALALETHEMVAGRFLNEADDIDRRKSAVVGQPVVDFLFKPGEKAIGEWIEVAG
                     VPFQIVGIFRNDGGAEQERQVYIPVSTAQLAFNGADSLGMLAFTAGNAGPEETQKVID
                     EVVAKLAERHQFSPTDKQAVMIQNNVEGFHRFQQMFFILSVFIVVIGLGSLAAGVVGV
                     SNIMMIAVKERTKEIGVRKALGATPSSIVFMVIQESVFLTGVAGLLGLAAGVGLLGLL
                     TTTVDPMLIAIAFQVALLFVIVAVLIVIAGFVRLFRLGYAARVAGLFAGLAVICALAG
                     IAGFVANVAKTDFIRNPSIDLSVGVTATLFLVMTGALAGYFPARAAARINPIHALRDE
                     "
     gene            1177..2436
                     /locus_tag="VFV99_09115"
     CDS             1177..2436
                     /locus_tag="VFV99_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5059507.1"
                     /translation="MTIFDLDKWKEIWATLRSNKLRTFLTAFGVFWGVFMLMAMLAFG
                     SSMQSGMQRQVKGMATNLLWVWGGVSTEAYEGMQPGRQTRYNTDDIELLRRLPQIEYL
                     APRLNLGGWMNSNAVTYGTKSGTFTIMGDYPELMHIMAFSYDAGRFINHRDIVERRKV
                     AVIGKKVREQLYPPDVDPIGTSIKISGVYFQVVGITGTLRSGQMGERDANTVFVPFTT
                     MKTAFNQGDRVGFFAMTAKPGTNGPELERQVRAALKQHHKIAPTDDMAIGSFNMFEKF
                     EGFATFMFVLAAMSWIVGGATLLAGVVGVSNIMLITVKERTKEIGVRKALGASPLSII
                     SMVMKESVVLTLLAGLVGISAGVGLMALADAVLANAVDMPFGPPNVGLPTVLLALGVL
                     IIAGMLAGIMPASHAASIKPIEALRAE"
     gene            2439..>3037
                     /locus_tag="VFV99_09120"
     CDS             2439..>3037
                     /locus_tag="VFV99_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5059508.1"
                     /translation="MKRVLAWGFGLLLVLIIVGAFAYLYKKSKAKPVIYKTETAETAD
                     ITKKTVATGSIVPRREVEVKPKVTGVLSELYVEPGKIVKMGDALGKISIIPDAMQINQ
                     ADSGLRTAQIAFDNAKRELQRNEELYKRGVVADAELQKYRTEFALRQQELTTAGQSLQ
                     LVKEGATRGQGKLSTLLVTATSDGMVNDVPVKVGFSVIQA"
BASE COUNT          556 a          954 c          979 g          548 t
ORIGIN      
        1 cgactacgac atgctgaaag gtgtcgcgag ggtcgagaat ccgaccggcc agttcttcat
       61 ccagggcagc cgcttcggcg gcacgccgat gatgacgaag cgcggcggca aggcgagctc
      121 gttccagatc aacgcggtcc atcacaacgc gctcgcgctc gagacgcacg agatggtcgc
      181 cggccgcttc ctgaacgagg ccgacgacat cgaccggcga aaatctgccg tcgtcgggca
      241 accggtcgtc gacttcttgt tcaagcccgg agagaaagcg atcggcgagt ggatcgaggt
      301 cgccggcgtg ccgttccaga tcgtgggtat cttccgcaac gatggcggtg ccgagcagga
      361 gcgccaggtc tacatcccgg tgtcgaccgc gcagctcgcg ttcaacggcg ccgacagcct
      421 cggcatgctc gcgttcaccg ccggtaatgc cggccccgag gaaacacaga aggtcatcga
      481 cgaggtcgtc gcgaagctcg ccgagcgcca ccagttctca cccaccgaca aacaagcggt
      541 gatgatccag aacaacgtcg agggctttca ccgcttccag cagatgttct tcatcctgtc
      601 ggtgttcatc gtcgtgatcg ggcttggctc gctcgcagcc ggtgtcgtcg gcgtgtcgaa
      661 catcatgatg attgccgtca aggagcgcac gaaggagatc ggtgtgcgca aagcgctcgg
      721 cgcaacgcca agctcgatcg tgttcatggt gatccaggag tcggtgtttc tgaccggcgt
      781 cgccggtttg ctcggactcg cggcgggtgt cggcctgctc ggcctgctca cgacgacggt
      841 cgacccgatg ttgatcgcca tcgcgttcca ggtcgcgctc ctgttcgtca tcgtcgccgt
      901 gctgatcgtc atcgccggct tcgtgcggtt gttccggttg gggtacgccg cgcgcgttgc
      961 cggactgttc gccggtctcg ccgttatctg cgcgctggcc gggatcgcgg gcttcgtcgc
     1021 gaacgtcgcg aagacggact tcatccgaaa tccgtcgatc gatctgagtg tcggagtcac
     1081 cgcgacgctg ttcctcgtga tgaccggcgc actcgctggc tacttccctg ctcgcgcggc
     1141 ggcgcgcatc aacccgatcc atgcactgag ggacgaatga cgatcttcga cctcgataag
     1201 tggaaagaga tctgggcgac gctgcgctcg aacaagctgc gcacgtttct caccgcgttc
     1261 ggcgtgttct ggggcgtgtt catgctgatg gcgatgctcg cgtttggctc gagcatgcag
     1321 agcggcatgc agcgccaggt caaaggcatg gcgacgaatc tgctctgggt ctggggcggt
     1381 gtgtcgaccg aggcctacga aggcatgcag ccaggccgcc agactcgcta caacaccgac
     1441 gacatcgagc tgctgcgccg gctaccgcaa atcgagtacc tcgcgccgcg cctcaacctc
     1501 ggcggctgga tgaacagcaa cgcggtcacg tacgggacca agagcggcac gttcacgatc
     1561 atgggcgact accccgagct catgcacatc atggcgttca gctacgacgc cggtcggttc
     1621 atcaatcacc gcgacatcgt cgagcgccgc aaggtcgccg tgatcggcaa gaaggtgcgc
     1681 gagcagctct atccccccga cgtcgatccg atcggcacgt cgatcaagat cagcggcgtt
     1741 tacttccagg tcgtcggcat caccggcacg ctgcgcagcg gccagatggg cgagcgcgac
     1801 gcgaacaccg tgttcgtgcc gttcacgacg atgaagacag cgttcaatca gggcgatcgc
     1861 gtcggcttct tcgcgatgac cgcgaagccc ggcacgaacg gtcccgagct cgaacgccag
     1921 gtgcgcgcgg cactcaagca gcaccacaag atcgcgccga ccgatgacat ggcgatcggg
     1981 tcgttcaaca tgttcgagaa gttcgagggc tttgccacgt tcatgttcgt gctagcggcc
     2041 atgagctgga tcgtcggcgg tgcgaccctg ctcgccggcg tcgtcggcgt gtcgaacatc
     2101 atgctgatca cggtgaagga acgcacgaaa gagatcggcg tgcgcaaggc gctcggcgcg
     2161 tcaccgctgt cgatcatctc gatggtgatg aaagagtcgg tcgtgctgac gctgcttgcc
     2221 ggtctcgtcg gcatctcggc aggcgtcggc ctgatggcgc ttgccgatgc ggtgcttgcc
     2281 aatgcggtcg acatgccgtt cggcccgccg aacgtcggcc tgccgacggt gctgctcgca
     2341 ctcggcgtgc tcatcatcgc cgggatgctc gccggcatca tgcctgcttc tcatgcagct
     2401 tcgatcaaac cgatcgaagc actgcgagcg gagtgactat gaaacgcgta cttgcttggg
     2461 gctttggcct gttactggtc ctcatcatcg tcggcgcctt cgcctatctg tacaagaagt
     2521 cgaaggccaa gccggtcatc tacaagaccg agacggccga gaccgccgac atcacgaaga
     2581 agacggtcgc gaccggttcg atcgtgccgc gccgcgaggt cgaggtgaag ccgaaggtca
     2641 ccggcgttct atccgagctc tacgtcgagc ccggcaagat cgtgaagatg ggtgacgcgc
     2701 tcggtaagat ctcgatcatc cccgacgcga tgcagatcaa ccaggccgac tccggcctgc
     2761 gcaccgcgca gatcgcgttc gacaacgcga agcgcgagct gcagcgcaac gaagagctgt
     2821 acaagcgcgg cgtcgtcgcc gatgccgagc tgcagaagta ccgcaccgag ttcgcactgc
     2881 ggcagcagga gctgacgaca gccggccagt cgctgcagct cgtgaaggaa ggagcgacgc
     2941 gcggtcaggg caagctgtcg acgctgctcg tcaccgcgac gtcggacggc atggtgaacg
     3001 acgtgccggt caaggtcggc ttctcggtga tccaggc
//
LOCUS       DASUFX010000233         6345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1424038, whole genome shotgun sequence.
ACCESSION   DASUFX010000233 DASUFX010000000
VERSION     DASUFX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424038"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..782)
                     /locus_tag="VFV99_09125"
     CDS             complement(159..782)
                     /locus_tag="VFV99_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5059509.1"
                     /translation="MSAAKPKPEGGQLVPLRRRDEQRAAVSDLSDEALLAACGVGDTA
                     ALGALFDRHHEAVYRLLSRLLRTEPDEIDDLVQLTFLEAWRAAKKYSGRGAVRSYLFG
                     IAANTVRHHIRGAARRRAALADAPVPTPASTPDAAAMRAQQVHRLAAALDELSHELRA
                     AYVLCDLEDVPGVEAARVLGIRAGTLWRRLHDARRALRDAIDEGGNP"
     gene            complement(787..1854)
                     /locus_tag="VFV99_09130"
     CDS             complement(787..1854)
                     /locus_tag="VFV99_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059510.1"
                     /translation="MAAARVALALLLAVSAAAAQTAGAEPCAPRAELGGDAEAVAKVA
                     AELQRLGVDASTDPTKPPARTSCPVIVAAVELDRGGGIAVAVRDASQRSEGRVVSDAA
                     LAAAWIESWLRDDFTAPPPPEVTPLAPPSTVAATPAPAVSTPLLDRFSLAASYLLGWS
                     DDSTSWSGFGTSLCVHAGGWCIGGRVGYENQTVTADLTAAAKSDLSLLATASYPRELG
                     TMSIAPEFGVGVGRASTARVEGCKLMDPNCDPMTDPMCMTMPPQPCTNPDGTVYVGDH
                     FSTATYTPRLSAALRIAVPLFDHVWLDGLAAATAAPFGHSDEYAPIAADGTMSNGAAY
                     PLPGDPMLGIQLGIGLRVGAR"
     gene            complement(1854..3281)
                     /locus_tag="VFV99_09135"
     CDS             complement(1854..3281)
                     /locus_tag="VFV99_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059511.1"
                     /translation="MTSGLKTWLFAACAVAGFACTDADKTQVLTGRVTARNAVTVRAI
                     DGDTIITASRVRSDGSFTLSLPSGHHYRLEVLTADGVHNIVGTKGGTIKDLAFKVCQP
                     QAPFDMGGMNQPCGPNDPNCMGGGGGNGCDPMTDPSCGLCPDGTWGSNCAPPCDPNSD
                     PNCMWPYPCDPSTDPMCPQPCDPTDPSCMKCDPMTDPSCGLCPDGTWGSNCMPPCDPT
                     TDPTCMWPPPPCDPSTDPMCPQPCDPSTDPNCAPCNPMTDPNCQPPPPCDPTTDPMCP
                     QPCDPSTNPNCAPPCNPMLDPTCGQCPDGTWGPNCVPPCDPTTDPMCKCYPGDPNCMQ
                     PPPCDPMTDPNCAPYPCDPNDQSCVICADGTMDPNCKCDWLNGTCEPCDPMVDPMCSL
                     PPPPCTDPMDPNTCKDPCANDPTNCGCTMDPVDPGTMDPTMPNSDGTTSSCWPPPEPC
                     TMESCPDGMSPEHQPGDFGCGDGTN"
     gene            complement(3376..4074)
                     /locus_tag="VFV99_09140"
     CDS             complement(3376..4074)
                     /locus_tag="VFV99_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5059512.1"
                     /translation="MSRERRQIRRGRRAERQERRAERIENRAVRQRRTPEAAKQLLLD
                     AAERLFADENPGDVGLKEVAREAGVSHALITHYFGTYAGLVEATLERRLATLRERIRE
                     RLGEAGALSRPEELLGMLFTALEDPVHLRLVKWLAASERATPMFALQHRGLQAIAQQV
                     AHAVRPNPPREMLETLELALLTAVAAATGYAISKAALAGAIGQEPNPALDAGVRKTLA
                     GMVQTYLRDRIGLA"
     gene            4160..4885
                     /locus_tag="VFV99_09145"
     CDS             4160..4885
                     /locus_tag="VFV99_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEX5059513.1"
                     /translation="MTNQPATLPDAADLPTLVRWHRAFRALSRVLMNPEETDQVLVFS
                     NLANAGQRDERIHLFYDNPRGKRLYDEKRTIDSHTVDLDALAALPAGTLGHAYATFMR
                     SHGLTPDVFDGRPADVRDDRSAYVIQRMRQTHDLWHVVTNAETDPAGEVALQAFTYAQ
                     MHAPSSGILAALGMLRTLRYTREVVRDVIDMYRLGRTAEPLAIFPWEDHWATPIVDVR
                     RMLKLPVNPRPIGGYTAELMEAA"
     gene            4976..5155
                     /locus_tag="VFV99_09150"
     CDS             4976..5155
                     /locus_tag="VFV99_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059514.1"
                     /translation="MRKPAGKARKLIVPVMLTVTIAATAATVATTAVTVLASAGCDDD
                     DPPKVDAGMPDTPLI"
     gene            5281..5616
                     /locus_tag="VFV99_09155"
     CDS             5281..5616
                     /locus_tag="VFV99_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059515.1"
                     /translation="MPSQDDRDPSSPRRLRIPKALVAALVGSSATLAVSFSCDEPKPK
                     VPVDAQEIVDEEGTIDASDIDGDMIADAMIDAKPDAKPDAKPDAMPDAKPDARPDARP
                     DARPDAPIV"
     gene            5742..6017
                     /locus_tag="VFV99_09160"
     CDS             5742..6017
                     /locus_tag="VFV99_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059516.1"
                     /translation="MGTHDEGTARPQPRRHVRVPVALAVTFVGSASAVSIWYGGCTQT
                     TDPPLDALHIANQPDASPVDAALDASDTTDAPRDAAPGAMPDGPPPQ"
     gene            6062..>6345
                     /locus_tag="VFV99_09165"
     CDS             6062..>6345
                     /locus_tag="VFV99_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059517.1"
                     /translation="MSTRWIRSSRAAVAEVGDELLLIGRDGSVQRLDDDGAELAREVL
                     LFFAQPHDEAELMAHVEMLAGPLGERRQVVLDLLELLRDTEAVEQARDVVP"
BASE COUNT         1040 a         2192 c         2108 g         1005 t
ORIGIN      
        1 aaacgcacca ccgatcacgt accagcgcgt cgatggacgt cgatcgccct cggcagctgc
       61 aacgagcagc cccgagcgca ccgcgtcgcg acgcactcca tcgggacgat cccacggcaa
      121 ctgcgtgccg agctcgcgca aacggtcgtc gtgctcgttc atgggtttcc tccctcgtcg
      181 atcgcgtcgc gcagtgcgcg tcgcgcatcg tgtaagcgtc gccatagcgt gccggcgcga
      241 atgccgagca cgcgtgccgc ctcgacaccc ggtacgtctt cgagatcgca cagcacgtag
      301 gctgcgcgca gctcatggga aagctcgtcg agtgcggctg cgagcctgtg cacttgctgc
      361 gcgcgcatcg ccgccgcgtc tggtgtgctc gcaggtgtcg gcacgggcgc atcggcgagc
      421 gctgcacgcc ggcgcgcggc accacgaatg tgatggcgca ccgtgttcgc ggcgatgccg
      481 aacaggtagc tacgcaccgc gccgcgcccg ctgtacttct tcgcggcacg ccacgcttcg
      541 agaaacgtga gctgcacgag gtcgtcgatc tcgtcgggct cggtgcggag cagccgcgac
      601 agcaagcgat acaccgcctc gtgatggcga tcgaacagcg cgccgagcgc agcggtgtcg
      661 ccgacgccgc atgcggcgag cagcgcttcg tcggagaggt cggacacggc tgcacgctgt
      721 tcatcgcggc gacgcagcgg aacgagctgg ccgccttcgg gtttgggttt cgcggcgctc
      781 atggagtcat cgcgccccca cgcgcaagcc gatgccgagc tggatgccaa gcatcggatc
      841 accgggaagt ggatacgcag cgccgttcga catcgtgccg tcggcggcga tcggtgcgta
      901 ctcgtcggag tggccgaacg gcgcggcggt cgctgccgcg agaccgtcga gccacacgtg
      961 atcgaacagc ggcaccgcga tgcgcagtgc cgccgatagg cgcggcgtgt acgtcgcggt
     1021 cgagaagtga tcgccgacgt acaccgtgcc gtcgggattc gtgcacggct gcggcggcat
     1081 cgtcatgcac atcggatcgg tcatcgggtc gcagttcgga tccatcaact tgcagccctc
     1141 gacacgcgcg gtcgatgctc gaccgacgcc gacgccgaat tcgggtgcaa tcgacatcgt
     1201 gccgagctcg cgcggatagc tcgcagtcgc gagcaacgac agatccgact tcgccgccgc
     1261 ggtgaggtcg gcggttaccg tctgattctc gtagccgacg cggccaccga tgcaccaacc
     1321 gcctgcgtgc acgcacagcg acgtgccgaa gcccgaccac gacgtgctgt catcgctcca
     1381 gccgagcaga tacgacgcag cgagcgagaa gcggtcgagc agcggcgtgg acacggcggg
     1441 tgccggcgtc gcggcgaccg tcgagggcgg tgcgagtggc gtcacttccg gtggcggcgg
     1501 cgcggtgaag tcatcgcgca gccacgactc gatccacgcg gccgcgagcg ccgcgtcact
     1561 gacgacccgg ccctcgctgc gttgcgacgc gtcgcgcacc gcgaccgcga tgccgccgcc
     1621 gcgatcgagc tcgacagcgg cgacgatcac cgggcagctg gtgcgtgccg gaggcttcgt
     1681 cggatccgtg ctcgcgtcga cgccgaggcg ctgcagctcg gccgcaacct tcgcgaccgc
     1741 ctcggcatca ccaccgagct cggcgcgtgg cgcgcagggc tccgccccgg cggtctgcgc
     1801 ggcagcggcg ctgacggcaa gcaagagcgc gagcgcgaca cgcgcagctg ccatcagttc
     1861 gtgccgtctc cgcagccgaa gtcaccgggc tggtgctcgg gcgacatgcc gtcggggcag
     1921 ctctccatcg tgcacggctc cggcggcggc cagcacgagc tcgtggtgcc atcgctgttc
     1981 ggcatcgtcg gatccatcgt gcccggatcc accggatcca tagtacagcc gcagttggtc
     2041 ggatcgttcg cgcacggatc cttgcaggtg ttcggatcca tcggatcggt gcacggcggc
     2101 ggcggcaacg agcacatcgg atcgaccatc ggatcgcacg gctcgcacgt accgttgagc
     2161 cagtcgcact tgcagttcgg atccatcgtg ccatcggcgc agatcacgca cgactggtcg
     2221 ttcggatcgc acggatacgg cgcgcagttc ggatctgtca tcggatcaca cggcggcggc
     2281 tgcatgcagt tcggatcgcc ggggtagcac ttgcacatcg gatccgtcgt cggatcgcac
     2341 ggcgggacgc agtttggacc ccacgtaccg tcggggcact gtccgcatgt cggatcgagc
     2401 atcgggttgc acggcggcgc gcaattcggg ttcgtcgacg gatcgcacgg ctgcgggcac
     2461 atcggatccg tcgtcggatc acacggcggc ggtggctgac agttcggatc cgtcatcgga
     2521 ttgcacggcg cgcaattcgg gtccgtcgac ggatcgcatg gctgcgggca catcggatcg
     2581 gtcgacggat cgcacggcgg cggcggccac atgcaggttg gatcggtcgt cggatcgcag
     2641 ggcggcatgc agttgctgcc ccacgtgccg tcgggacaca gaccgcagga cggatccgtc
     2701 atcggatcgc acttcatgca gctcggatcg gtcggatcgc acggctgcgg gcacatcgga
     2761 tcggtcgatg gatcgcacgg atacggccac atacagttcg gatccgaatt cggatcgcac
     2821 ggcggcgcgc agttgctgcc ccacgtgccg tcgggacaga ggccacacga cggatccgtc
     2881 atcggatcgc aaccgttgcc gccaccacca cccatgcagt ttggatcgtt cggaccgcac
     2941 ggctggttca tgccacccat gtcgaacgga gcctgcggct ggcagacctt gaacgcgagg
     3001 tctttgatcg tgccgccctt cgtgccgacg atgttgtgca caccatcggc ggtgagcacc
     3061 tcgaggcgat agtgatggcc ggagggtaat gacaacgtga acgagccgtc gctgcgcacg
     3121 cggctcgcgg tgatgatcgt gtcgccgtcg atcgcgcgca cggtgaccgc gttcctcgcc
     3181 gtcacgcgac ccgtcagcac ctgcgtcttg tccgcgtcgg tgcaggcgaa cccggcgacc
     3241 gcacaggcgg cgaacagcca ggtcttcaat ccgctggtca tggctcctcc tcttggctct
     3301 ctcacgccgg ctagtgcccg caaagcggaa agccttcgca gaaatgtgcg ggcatgtggc
     3361 cgcccgcacg ccggactagg cgagcccaat gcgatcgcgg agataggtct gcaccatgcc
     3421 ggccagggtc ttgcgcactc ccgcgtccag cgccggattc ggctcctgtc cgatcgcgcc
     3481 ggcgagcgcc gccttgctga tcgcgtagcc cgtcgctgca gcgacggcgg tgaggagcgc
     3541 gagctcgagc gtctcgagca tctcgcgggg cggattcggg cgcaccgcgt gcgcgacctg
     3601 ctgcgcgatc gcctgcaggc cgcggtgctg gagcgcgaac atcggcgtcg cgcgctcgct
     3661 cgcggcgagc cacttcacga gccgcaggtg caccggatct tcgagcgcag tgaacagcat
     3721 gccgagcagc tcctcgggac gcgacagcgc acccgcctcg ccgagccgtt cgcgaatgcg
     3781 ctcgcgcagc gtcgcgagcc ggcgctcgag cgtcgcttcg acgagcccgg cgtaggtccc
     3841 gaagtaatgc gtgatcagcg cgtgactcac gcccgcttcg cgagcgacct ctttgagccc
     3901 gacgtcgccg ggattctcgt cggcaaacag ccgctccgcc gcgtcgagca ggagctgctt
     3961 cgcggcctcg ggtgtgcgcc gctggcggac cgcgcggttt tcgatccgct ccgcacggcg
     4021 ttcctgccgc tcggcccgcc gtccgcgccg gatctgcctg cgttctcgcg acattgctga
     4081 ccgtgacgct cgcacattat tttcacgttg acaataagtg cttctggtcc tattttcatc
     4141 gtgacaataa ggactggcca tgaccaacca acccgccacc ctccccgacg ccgctgacct
     4201 ccccaccctg gtgcgctggc accgcgcgtt tcgcgcactg tcccgcgtcc tcatgaatcc
     4261 cgaggagacc gaccaggtcc tcgtgttctc gaacctcgcg aacgcgggcc agcgcgacga
     4321 acggatccat ctcttctatg acaacccgcg cggcaagcgg ctctacgacg agaagcgcac
     4381 gatcgattca cacaccgtgg atctcgacgc gctcgccgct ctgcccgccg gcacgctcgg
     4441 tcacgcctac gcgacgttca tgcgctcgca cggtctgacg cccgacgtgt tcgacggtcg
     4501 tcccgctgac gtgcgcgatg atcgctctgc gtatgtcatc cagcgcatgc gccagacgca
     4561 tgacctgtgg cacgtcgtga cgaacgccga gaccgatccg gccggcgaag tcgcgctgca
     4621 agcattcacc tacgcgcaga tgcacgcgcc atcgagcggc atccttgccg cactcggcat
     4681 gctccgcacg ctccgctaca cgcgcgaggt cgtgcgcgac gtgatcgaca tgtaccgcct
     4741 cggcagaacc gccgagccgc tcgccatctt tccctgggaa gaccactggg ccacgccgat
     4801 cgtcgacgtg cgccggatgt tgaagcttcc cgttaaccct cgcccaatcg gcggctacac
     4861 cgccgagcta atggaggcag catgaagaat cgtgaacaaa gactcgcccg ccgcaacgcc
     4921 cgccgcatcc gtcgtttgat gcgcaagctc acgcgcgtgg tgttacgctc gacgcgtgcg
     4981 taagccagcg gggaaggcac gcaagctgat cgtgcctgtg atgctcaccg tgacgattgc
     5041 ggcaaccgct gcgaccgtcg cgaccaccgc ggtaaccgtg ctcgcgagcg caggctgtga
     5101 cgacgacgac ccaccgaagg tcgacgccgg catgcccgat acaccgctca tttgatagac
     5161 gcatgcggcc cggtcgggct gcacgcagtt tgatgttcct ctcgcaggag cgacggcggc
     5221 gcacgctgtg cagatcaatc gcacagctgc gcgctctcgg aggacgacct ataccgcaac
     5281 atgccctcgc aagacgatcg cgacccctcg tcgccgcgtc gcctgcgaat tccgaaagcg
     5341 ctcgtcgcag cgctcgtcgg ttcgtcggcg acgttggcag tttcgttctc gtgtgacgag
     5401 ccgaaaccga aggttcctgt cgacgcacag gagatcgtcg atgaagaggg cacgatcgac
     5461 gccagtgaca tcgacggcga catgatcgcc gacgcgatga tcgacgcgaa gccggacgcg
     5521 aagcccgatg cgaagcccga cgcgatgccg gatgccaagc cggatgcgcg ccccgatgcg
     5581 cggccagacg cgcgacccga cgcgccaatt gtttgatgac caccggcggc ccggtcgggc
     5641 tgcgagacgt ctgagctaga acgcgatcac cgcgcgccgt cgctcgctgt gcagttggtg
     5701 cgcacagctg cgtaccggca acgcgcatgc gatacccgat catgggtacg cacgacgagg
     5761 gcaccgcacg gccacagccg cgtaggcacg tgcgcgttcc cgtcgcgcta gccgtcacgt
     5821 tcgtcggtag cgcgagcgcg gtcagcatct ggtacggcgg ctgtacgcag accaccgatc
     5881 cgccgctcga cgccctgcac atcgcgaatc aaccagatgc atcgccagtc gacgccgcgc
     5941 tcgacgcgag cgatacgacc gacgcgccgc gcgatgccgc gcccggcgca atgccggacg
     6001 gaccaccacc acaatgatga gcccgggcgg cccggtcggg ctgcgagtgc tagaccgacg
     6061 agtgagcacc cgctggattc gctcgtcgcg agccgcggtc gctgaagtcg gcgacgagtt
     6121 gctgctgatc ggccgcgacg ggagcgtgca acggctcgac gacgacggcg ccgagctcgc
     6181 gcgcgaggtg ctgctgttct tcgcgcagcc gcatgacgaa gcggagctga tggcgcacgt
     6241 cgagatgctc gccggtccgc tcggtgagcg gcggcaagtc gtgctcgatc tgctcgagct
     6301 gttgcgcgac accgaggcgg tcgagcaagc gcgcgatgtc gtacc
//
LOCUS       DASUFX010000234         7618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_173963,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000234 DASUFX010000000
VERSION     DASUFX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173963"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFV99_09170"
     CDS             complement(<1..1173)
                     /locus_tag="VFV99_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX5059518.1"
                     /translation="MSLREAVRAEATERTWSQGVTLAREQRVIGRGRSGDEVELEVRV
                     PGRPTPFEVVLDEFNEEWQCTCPSKEAVCSHVVAAVLATEQARQSGSESLPTPTKLGA
                     PIRYLLSPDPAGVRVERVLVRGDNDEPLPGPLMSLVANGRASGIATIEADLLADQLLG
                     VRATPVTGEKLDRLLEVLADARDVRWRGDPVTTNGEPVLPRAVVEDMPDGVRVRIERD
                     PIVRDVPAVGVVRTTDNVLRPIGAVDLSGPRLDKLPQIFDVPRAAIPELMGKTLPALA
                     QRIDVDVRATALPRVGAREEPRMQIDVAQDGDKLVVMPTLVYGDPPRARVDGKTLVHI
                     EGTLPIRDEDAERRLLHRLRDELNLVPGRRVELVGRDAFSMQQALGTWLRHDAKSAA"
     gene            1225..1764
                     /locus_tag="VFV99_09175"
     CDS             1225..1764
                     /locus_tag="VFV99_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5059519.1"
                     /translation="MVPDTDAEDAALVAAMARGDREALAKLYERHAGVLLGLALRIVR
                     EPREAEDLLHDVMLEAWRAAKDFDPKRGRVRTWLAIRMRSRAIDLQKSARVSRNAGDA
                     GIEAVVDERERTSPDHVRVRDALAELGPDQRQVLELAYFEGLSCSEIAARIAIPIGTV
                     KSRLAAGMQRLRERIGGTR"
     gene            1761..2354
                     /locus_tag="VFV99_09180"
     CDS             1761..2354
                     /locus_tag="VFV99_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5059520.1"
                     /translation="MTNVRDLLPLYALGELTADEAATVERAIEGDANLAAELESYLEL
                     VAVAPPADIKARLLASIGGGRFEKFADRMATLLDVTIDRARELLGLVERPASWENPVP
                     GVSLIHFEGGPAYAAADCGFVRIAPGCKFPWHTHRGEEVSVILSGTMRDVDGRMLGAG
                     DELVHQQGTQHELTNDGDADVIYVARAMNGIEVRPPQ"
     gene            2388..2684
                     /locus_tag="VFV99_09185"
     CDS             2388..2684
                     /locus_tag="VFV99_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059521.1"
                     /translation="MADDAGLRLVGTIRTIELHTLAARFEHVATRQVAKIQLDIERAI
                     DEEGEELDPVNLADLHFQGPAELVPRFSAGDRVQIVTSPESSLQISSIRPAPLS"
     gene            complement(2707..4401)
                     /locus_tag="VFV99_09190"
     CDS             complement(2707..4401)
                     /locus_tag="VFV99_09190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059522.1"
                     /translation="MTIPAGTLVPRDARDFGSYQLLAKLATGGMAEIYLARPTSSQSG
                     SKDMIVLKRILPHLAEDDHFVTMFRDEADLAAKLVHKNVCHVSSFGQYGGTWFIAMEY
                     LHGVPLSRMMTKLSKAGKMLDYRIVAGIICQACEGLHSAHEARDDKGALMGVVHRDVS
                     PPNIMVTADGTVKLLDFGIAKARGANSRTRTGTVKGKNAYMSPEQILGKPLDRRSDIF
                     ALAVVMYEMLAIKRLFHRDSDFLTFKAITEEPIPEIRDRRPDLPPGMRAALVQAMARD
                     PAGRFDTAQGFGNAIKNSVATLGGPASPADLARILFTDFADEMASRDEILKAADDPNA
                     APISIGPAKPKAPPPIPQRPAQLATSGEMANQTPARSKTPTGSLGVPSMIVHSGGTGK
                     IELPDLGNVLDLSADVGADAWMVEGGTDLLSSHRLKSLRNLAIAFLAVAAAVFGIWYA
                     VSKLGGSSETPVVHVPADAAVKRTYVPEQPKPDAPEMSKDDIVAISKFGFFSINASAK
                     TTIYVDSVRYGDTPMQRLPLTPGPHKVKAVTKGKKPKEWMITIIGGRDTDEGTITW"
     gene            complement(4434..4952)
                     /locus_tag="VFV99_09195"
     CDS             complement(4434..4952)
                     /locus_tag="VFV99_09195"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HEX5059523.1"
                     /translation="MTPFCFEHVFVADGPGELFAAYFDVQHQIEQDRVLEIREREVIE
                     LFERGDEIVRRSRVVPSRQLPALLRPLASGPLHYIEHAVGRRAAHMIDIDLRLFGDRG
                     RVTARYELAQMAPGSVRRRYSGVVSVDVALVAGRVERGIVTEFERSLSRASSCTQNWL
                     VQQTPRSAAARA"
     gene            5108..5986
                     /locus_tag="VFV99_09200"
     CDS             5108..5986
                     /locus_tag="VFV99_09200"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX5059524.1"
                     /translation="MDSEYTGVDADRPRWRENIPFWGVHVAALVGAIMVGWSWSAFWW
                     LAGTYAVRMFAITAGYHRYFSHRTFKTSRVFQFILAVLGMTAAQQGPLWWAAHHRRHH
                     KYSDMPEDIHSPRQRGFIWSHMQWFVAKRHKATDYERIKDFAKYPELRWLNDHDLFFV
                     VALGFVMYFGFGANALFYGHFLSIVLAWHITFCINSVAHVLGKRRYKTTDDSRNNLTL
                     ALLTFGEGWHNNHHHYQRSARQGFYWWEIDVSWYVLKLLEALRIVWDVEGVPRHVRDQ
                     VEAPERERVEAIRSAA"
     gene            complement(6317..>7618)
                     /locus_tag="VFV99_09205"
     CDS             complement(6317..>7618)
                     /locus_tag="VFV99_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059525.1"
                     /translation="SVAAACSFSHGVLPDDGGVVDMPLPDAAKCLGSSTECLGDVLRT
                     CPGAGSDAFDTPCGWGCMPGSAARCLQIVPSGSGGTATNGVLVTDVAADAALSDITIP
                     GGVTFDSDLGNIGTNVMPTMFHSAATGVDKGIDFQYRGPISVWRFKSLTISGNVTLVG
                     KRPIALVADGPITINGVIDARGGCLAFNAGPGGFGGGSNQSEPGLPTVGGGSGSPDNT
                     SGGGGGGHGGSGATGNGVAGGSAFGDDLISTLVGGGGGGAGKCGNNNCDGGGGGGALQ
                     IVSNTRIVVQTGGVNAGGCGGKQGTGNGDTGGGGGAGGAILFEAPTVTIAAALAANGG
                     GGGGGGGNGSTTAGGNGSLDRTPAAAGTGNSNGEGGGSGAAANIAPGPGGNTGNPGGG
                     GGGIGRIRINTKDNAGATLTGATLSPGMSEPAFSTGSAALQ"
BASE COUNT         1234 a         2555 c         2498 g         1331 t
ORIGIN      
        1 ggcggcggac ttcgcgtcgt gacgcagcca tgtaccgagc gcttgctgca tcgagaacgc
       61 atcgcgaccg acgagctcga cgcggcggcc cggcacgaga ttcagctcgt cgcgcaggcg
      121 gtgaaggagt cgacgttcgg catcctcgtc gcgaatcggc agtgtgccct cgatgtggac
      181 gagcgtcttg ccatcgacgc gcgcgcgtgg cggatcgcca tagacgagcg tcggcatcac
      241 gacgagcttg tcgccgtcct gggcgacatc gatctgcatg cgcggctctt cgcgcgcgcc
      301 gacgcgggga agggcagtcg cgcggacgtc gacatcgatc cgctgcgcga gcgcaggcag
      361 cgtcttgccc atcagctcgg gaatcgcggc gcgtgggacg tcaaagatct gcggcagctt
      421 atcgaggcgg gggccgctga gatcgacggc accgatcggc cgcagcacgt tgtctgtcgt
      481 gcgcacgacg ccaaccgcgg gaacatcgcg cacgatcgga tcgcgttcga tgcgcacgcg
      541 tacgccatcc ggcatgtctt cgacgaccgc acgtggcagc actggctcgc cgttcgtcgt
      601 caccgggtca ccgcgccagc ggacgtcgcg cgcgtcggcg agcacttcga gcaagcgatc
      661 gagcttctcg ccggtcaccg gcgtcgcccg cacgccgagc aattgatcgg cgagcagatc
      721 ggcttcgatc gtcgcgatgc cactcgcacg gccgttcgcg acgagcgaca tcagcgggcc
      781 ggggagcggc tcgtcgttgt caccccgcac gagcacgcgc tcgacccgca cgcctgcagg
      841 atcgggcgac agcaggtaac gaatcggtgc gccgagcttg gtcggcgtcg gcaagctctc
      901 gcttccgctc tgccgcgctt gctccgttgc gagcacggcg gcgacgacgt gcgagcacac
      961 cgcctccttg ctcggacagg tgcactgcca ttcctcgttg aattcatcga gcacgacctc
     1021 gaacggcgtc ggccggccgg gcacacggac ctcgagctcg acctcatctc ccgagcgacc
     1081 gcggccgatg acccgctgct cgcgcgcgag tgtgactccc tgcgaccagg tgcgttcggt
     1141 cgcttcggct ctaacagcct ctcgcaggga catcgtcggg gccctttacc acgcaagcga
     1201 ccggcatgtt agggtgccga gcgcatggtg cccgacaccg acgccgagga cgccgcgctc
     1261 gtcgcagcga tggcgcgcgg ggaccgcgag gccctggcga agctgtacga gcggcatgcg
     1321 ggagtcctcc tcgggttggc gcttaggatc gttcgcgagc cgcgcgaggc ggaggatcta
     1381 ctacacgacg tgatgctcga agcctggcga gctgcgaagg attttgatcc gaagcgcggt
     1441 cgcgttcgta cttggttggc tattcgtatg cgctcgcgag ccatcgacct ccagaagtct
     1501 gcccgggtgt cccgcaatgc gggcgacgcg ggtatcgagg cggtggtcga cgagcgcgag
     1561 cggacgagcc ccgatcacgt gcgtgttcgc gacgcactcg ccgagctcgg acccgaccaa
     1621 cgtcaggtcc tcgagctcgc gtatttcgag ggactctcgt gttcggagat cgcggcgcgc
     1681 atcgcgattc ccatcggcac ggtgaagtct cgcctcgcag ccggcatgca gcggctacgt
     1741 gaacggatcg gaggtacgcg atgacaaacg tccgcgactt gctgccgctc tacgcgctcg
     1801 gcgagctcac tgccgacgag gctgccaccg tcgagcgtgc gatcgagggc gacgcgaacc
     1861 tcgccgccga gctcgagagc tatctcgagc tcgtcgcagt cgcgccgccg gcagacatca
     1921 aggcgcgctt gctcgcgtcg atcggtggcg gccgcttcga aaagtttgcc gatcggatgg
     1981 caacgctcct cgacgtcacg atcgatcgcg cgcgcgagct gctcggactc gtcgaacggc
     2041 ccgcatcgtg ggagaacccg gtgccgggtg tcagcttgat ccacttcgag ggtggtccgg
     2101 cctatgccgc cgcagactgc ggcttcgtgc gtatcgcacc tggctgtaag ttcccgtggc
     2161 acacgcaccg cggcgaagaa gtgtccgtga tcctgagtgg cacgatgcgc gatgtcgatg
     2221 gccgcatgct cggcgccggc gacgagctcg tgcatcagca agggacgcag cacgagctga
     2281 ccaacgatgg tgacgctgac gttatttacg tagcgcgcgc tatgaacggg atcgaagtcc
     2341 gcccgcccca atagccgtcg catgttgcgc aggcgtagta tggaatcatg gcggacgatg
     2401 cgggtctgcg attggtcggg accatccgga ccatcgagct ccacacgctc gctgcgcgct
     2461 tcgagcatgt agcgacgcgg caggtcgcca agattcagct cgatatcgag cgcgcgatcg
     2521 acgaagaagg cgaagagctc gatccggtga acctcgccga tctgcatttc caaggtcccg
     2581 ccgagctggt gccgcgtttc agcgcgggcg atcgcgtgca gatcgtgacg agcccggagt
     2641 cgagcctcca gatctcgagt attcgtccgg cgccgctgtc ctagcggcgg cgacgcacgg
     2701 cgagcgctac caggtgatcg tgccttcgtc ggtatcgcga ccgccgatga tcgtgatcat
     2761 ccattccttg ggcttcttgc ccttggtgac ggctttgacc ttgtgcggtc ctggcgtcag
     2821 cggcagacgc tgcatcggcg tgtcgccgta gcgcacgctg tcgacgtaga tcgtcgtctt
     2881 cgcgctcgcg ttgatgctga agaaaccgaa cttcgagatc gcgacgatgt cgtccttcga
     2941 catctccggc gcgtcgggct tcggttgttc cggaacgtag gtgcgtttga ccgctgcgtc
     3001 ggcaggcacg tgcaccaccg gcgtctcgct gctgccgccg agcttgctca ccgcatacca
     3061 gatgccgaac accgctgcgg cgaccgcgag aaatgcgatc gcgagattgc gcagcgactt
     3121 caaccgatgc gacgagagca gatcggttcc gccctcgacc atccacgcgt cggcgccgac
     3181 gtcggcgctc agatcgagca cgttgccgag atcaggcagc tcgatctttc cggtgccgcc
     3241 cgagtgcacg atcatcgacg gcacgccgag cgagccggtc ggcgtcttgc tgcgcgctgg
     3301 cgtctggttt gccatctcgc cgctcgtcgc gagctgtgcg ggacgctgcg ggatcggcgg
     3361 cggtgccttc ggtttcgccg gtccgatcga gatcggcgcc gcgttcggat cgtccgcggc
     3421 cttgaggatc tcgtcgcgcg acgccatctc gtccgcgaag tcggtgaaca gaatgcgcgc
     3481 cagatccgca ggcgacgcgg gaccgccgag cgtcgcgacc gagttcttga tcgcgttgcc
     3541 gaaaccttgc gcggtgtcga agcgaccagc cggatcgcgc gccatcgcct gcacgagcgc
     3601 agcgcgcatg ccgggcggca gatcgggccg gcgatcgcga atctcgggaa tgggctcctc
     3661 ggtgatcgct ttgaacgtga ggaagtcgga gtcgcgatgg aacaggcgct tgatcgccag
     3721 catctcgtac atcacgaccg cgagcgcaaa gatgtcgctg cgccgatcga gcggcttgcc
     3781 gaggatctgc tcgggcgaca tataggcgtt cttgcctttg acggtgccgg tgcgcgtgcg
     3841 tgagttcgca ccgcgtgcct tggcgatgcc gaagtccagc agcttcaccg tgccgtcggc
     3901 cgtgaccatg atgttcggcg gtgacacgtc gcggtggacg acgcccatca gcgcgccctt
     3961 gtcgtcgcgc gcttcgtgcg cggaatgcag gccctcgcac gcctggcaga tgatgcccgc
     4021 gacgatgcgg taatcgagca tctttccggc cttcgagagc ttcgtcatca tgcgcgacag
     4081 cggtaccccg tgcaggtact ccatcgcgat gaaccacgtg ccgccgtatt gcccgaacga
     4141 cgagacatga cagacgttct tgtgaacaag cttggcggcg aggtcggcct cgtcgcggaa
     4201 catggtgacg aagtgatcgt cctccgcgag gtgcggaagg attcgcttca gcacgatcat
     4261 gtctttgctg ccggattgtg acgacgtcgg tcgcgcgaga tagatctccg ccatcccgcc
     4321 cgtcgcgagc ttcgcgagga gctgatagct gccgaaatct cgggcgtcgc gcgggactaa
     4381 cgtccccgcc ggaatcgtca cagcggatat tatgcacgag gaaaatcccg gatttaagcc
     4441 cgggccgccg cagaacgtgg cgtttgttgc actagccagt tttgcgtaca ggaactggca
     4501 cggctcagcg accgctcgaa ttcagtcacg atcccacgct cgacacggcc agcaaccagg
     4561 gcgacatcca ccgacaccac acccgaataa cgccgacgta ccgatcccgg cgccatctgc
     4621 gccagctcgt agcgggcggt gacccgacct cgatcgccaa acaggcgcag gtcgatgtcg
     4681 atcatgtggg cggcacgccg gccgaccgca tgctcgatgt agtggagcgg ccctgacgcc
     4741 agcggccgta gcagcgcggg cagctgccgg ctagggacga cgcgcgaacg acgcacgatc
     4801 tcatcgccac gctcgaagag ctcgatgacc tcgcgctctc tgatctcgag cacccggtcc
     4861 tgctcgatct ggtgctgcac gtcaaagtac gccgcaaata gttcgccggg cccgtctgcg
     4921 acaaatacgt gctcgaaaca gaacggcgtc atgcgtcatg agtattcacg ttcgccacgc
     4981 gagctgacat cacagcttcg tatcagcgcg actcggtcat gaatccgtca cgaccgaggc
     5041 gattcatacc gcaatgctgc tgtaaccctg accgctttgg cgcgctgcgc gcctcacact
     5101 ggtctgcatg gattcggagt acaccggcgt agacgcggat cgaccgcgct ggcgcgagaa
     5161 cattccgttc tggggcgtgc acgtggcggc gctggttggc gcgatcatgg tcggctggag
     5221 ctggagcgcg ttctggtggc tcgccggcac ctatgccgtg cgcatgttcg cgatcaccgc
     5281 gggctaccac cggtacttct cgcaccggac gtttaagacc agtcgcgtct tccagttcat
     5341 cctcgccgtg ctcggcatga ctgccgcgca gcagggcccg ctgtggtggg cggcgcatca
     5401 tcgtcgccat cacaagtaca gcgacatgcc cgaggatatt cattcgccac ggcagcgcgg
     5461 cttcatctgg tcgcacatgc agtggttcgt cgcgaagcgt cacaaggcga ccgactacga
     5521 gcgcatcaag gacttcgcga agtatcccga gctgcgctgg ctcaacgatc acgatctgtt
     5581 cttcgtcgtc gcgcttggct tcgtcatgta cttcggcttc ggcgcgaacg cgctgttcta
     5641 cggccacttc ttgtcgatcg tgctcgcgtg gcacatcacg ttctgcatca actcggtcgc
     5701 gcacgtgctc ggcaagcgcc gctacaagac gaccgacgac tcgcgcaaca acctgacgct
     5761 cgcgctgctc acgttcggcg agggctggca caacaaccat caccactatc agcggtcagc
     5821 gcgccagggc ttctactggt gggagatcga tgtcagctgg tacgtgctca agctgctcga
     5881 ggcgctgcgc atcgtgtggg atgtcgaagg cgtgccgcgc cacgtgcgcg atcaagtcga
     5941 ggcgcccgaa cgcgagcgcg tcgaagcgat tcgctcggcc gcgtaaccgc ctccgcgatc
     6001 gcgtcggtct cgacgtcagg cgcgttcgcg ccatcgcgct gcagagcgcg aacacgcgag
     6061 ctgcatgccg cggtggggca ctcgcgccgc cgcatgggac ggagtgcacg cactacccat
     6121 cgacgcggcg ctccatgtcg ttccggcgca cgactgagcc gtctccccgc tacggcgctt
     6181 cgccccccac ctgcggcatg catctcgcgt tgacgagcta gcggggcagc gccattccgc
     6241 gcagagcccg cgtcgccatc acagcgctgg cgttcatgag cgcgagcgcg tcgaagcgat
     6301 tcgctcggca gcgtagttac tgcagcgccg cgctgcccgt cgagaatgcg ggttcgctca
     6361 taccggggct gagcgtcgct ccggtgagcg tcgcgccggc gttgtctttg gtgttgatgc
     6421 gaatgcggcc gatgccgccg ccaccaccgc cgggattacc ggtgttgccg cctggaccag
     6481 gagcgatgtt ggccgcggcg ccactgccac caccttcgcc gttgctgttg ccggtgccgg
     6541 cagcagctgg ggttcgatcc agcgagccgt tgccgcccgc ggtcgtacta ccgttaccgc
     6601 caccgccacc accaccgcca ccattcgcag cgagagctgc cgcaatggtc accgtcggtg
     6661 cttcgaagag gatcgcgcca cctgcgccgc caccaccgcc ggtgtcgccg tttccggtgc
     6721 cttgcttgcc gccgcagccg cccgcattga cgccgccggt ttgcacgacg atgcgtgtgt
     6781 tcgacacgat ctgcagcgca ccaccgccgc cgccgccgtc gcagttgttg ttgccgcact
     6841 tgccggcgcc accaccgcca ccgccgacga gtgtcgagat caagtcgtca ccgaatgccg
     6901 agccaccggc gacaccgttg ccggtcgcgc cgctgccgcc gtgtccaccg ccgccgccgc
     6961 cgctggtgtt atccggcgag ccgctaccgc cgccgaccgt cggtagacca ggctcgctct
     7021 gattcgatcc gccgccaaat ccgccggggc ctgcgttgaa cgcgaggcaa ccgccgcgcg
     7081 catcgatcac gccgttgatc gtgatcgggc cgtcggcaac gagcgcgatc ggccgcttgc
     7141 cgacgagcgt cacgttgccg ctgatggtca ggctcttgaa tcgccagacc gagatcgggc
     7201 cgcgatactg gaagtcgatg cccttatcga cgccggttgc tgcgctgtgg aacatcgtcg
     7261 gcatgacgtt cgtgccgatg ttgccgaggt cgctgtcaaa cgtgacgcca ccgggaatcg
     7321 tgatgtcgct cagcgctgcg tccgccgcga catcggttac gagcacgccg ttcgtcgcgg
     7381 tgccgccgct gcccgatggg acgatctgta agcaacgagc ggcggagccg ggcatgcagc
     7441 cccagccgca tggcgtgtcg aacgcgtcgc tgcccgcgcc cgggcaggta cggagcacgt
     7501 cgccgaggca ctccgtgctg ctgccgaggc acttggccgc gtcgggcagc ggcatgtcga
     7561 cgacaccgcc gtcgtcgggc aacacgccat gcgagaagct acaggctgcg gcgacgct
//
LOCUS       DASUFX010000235        11694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1564562, whole genome shotgun sequence.
ACCESSION   DASUFX010000235 DASUFX010000000
VERSION     DASUFX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564562"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFV99_09210"
     CDS             <1..500
                     /locus_tag="VFV99_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5059526.1"
                     /translation="HMVGAVLSLAGVAVLFLDRLDVSTEQAVGVALVIGSVLLATSYS
                     MVMKRQTTGGLVATTVFLGVTALALSVVAIVAGEPIPWPPPLEPTLALLYLAIVGSVI
                     AFLLYFWMLNRTSLVVTSTLVFVFPLVALVTDAMFEREIALGPRAYLGAAITLGGLAV
                     TLRRK"
     gene            571..2529
                     /locus_tag="VFV99_09215"
     CDS             571..2529
                     /locus_tag="VFV99_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX5059527.1"
                     /translation="MSDVKRDHPWIFRTYAGHSSARASNELHRKNLAAGQTGLSIAFD
                     LPTQCGYDADHPLARPEVGKVGVPIASLDDMHALFDGIPIEQMNTSMTINGTAMWLLA
                     LYVALARERGVPEAGLRGTTQNDIVKEYLARGTYIFPPEPSLDLIAETYEYCVANLPQ
                     WNPSNICSYHLQEAGATPVQELAFALADAIGVLDRVKARGRVDSAAMAQAVGRVSFFV
                     NAGMRFVEEMCKMRAFGELWDDICSKRYGVTDPKLRQFRYGVQVNSLGLTEQQPENNA
                     WRILIEALGVTLSKDARCRALQLPTWNEALSLPRPWDQQWSLRLQQILAYETDLLEYG
                     DLFTGNPVVERKVAALCDAARTELAQIDGMGGIRGAIESGYCKRELVRSMAQRMARIE
                     RGEQTVVGVNKWTEGLQSPLVGGDDGGVFKVDPAAVDAAFASLEQTRKRRDAGNVKAA
                     LAELEAKARKGEPIMETSIQCALARVTTGEWAGTLRKVWGEYRAQTGVQGARIADGDG
                     WNALRARVEAHVAKTGRRPRLLVGKPGLDGHSNGAEVIAVAAKDAGFEVIYAGIRLEP
                     AAIAQTAVQEAVDVVGLSVLSGSHVELAKAVLEELRTRGGSDVAVVLGGTVPASDHAS
                     LRELGVRRIFTPSDYRLIDVVGALLDLC"
     gene            complement(2569..3189)
                     /locus_tag="VFV99_09220"
     CDS             complement(2569..3189)
                     /locus_tag="VFV99_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5059528.1"
                     /translation="MRLGCLCIALVAACGFEHGTSVTRDDAPSTLDDVLAAVDAPGDT
                     MNVIVDAPPDSSLPPPPCSTTGLICVGTTVAMNCNGACWVGCTQTANVPNQDHAVTAC
                     TAWGGKLAPIRTQADQNCVEQIFQGELNWIGFEQSSTATSLTTGWTWNSDGLTPTYTH
                     WAAGQPNDVNGNENDHAEQCAFMDDNGTWGDSQCGNAAYFRFSCRK"
     gene            complement(3239..3814)
                     /locus_tag="VFV99_09225"
     CDS             complement(3239..3814)
                     /locus_tag="VFV99_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5059529.1"
                     /translation="MMLLAACSFEHGTSIFDAPGQSRDGLLDAPSDTTNVTIDAAPEC
                     SSSGLVCAGVTSSLMCNGACWAKCTQIGVSVPSQGTAVLSCANWGGKLAPIRDQGDQD
                     CVAQMLFPGQSHWIGFEQSSSATSLTTGWTWNSDGIAPTFTHWAAGQPNDTDGTETDH
                     AEQCATMNTNGDWHDTPCDDDNSYRFSCRKN"
     gene            complement(3876..4433)
                     /locus_tag="VFV99_09230"
     CDS             complement(3876..4433)
                     /locus_tag="VFV99_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5059530.1"
                     /translation="MRRVAVVLVLIGCGRFGFDRGPSRDDGAVPPSEAEIAIDAQLTA
                     DAPMLALDAGQCPATYMKLGTSCYRFSVTQVPTWLDGELACEADGVGAHLVTIDDATE
                     GNLLATSIVINDYWAGMTDRITEGTYRNVTGEAAPYLVWVSGEPTSSDCSQFDDGGRF
                     HVSSCDTSDEYLCEYDGRPAVAGAY"
     gene            complement(4463..5848)
                     /locus_tag="VFV99_09235"
     CDS             complement(4463..5848)
                     /locus_tag="VFV99_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059531.1"
                     /translation="MSRDHKRELTRRALIKWSVAAGAALGVSQTRLLEILERHAGRGV
                     AQAAATTPTKRSVHIRGVNGGIAWFTLLWPHNAVAAVAGGSTSTWPFAAAQTQKIQGT
                     GGALTLGPNTPFANLPANQQMTAFMAGSNEAHTPNPNSIVRSVNGNSLFAIVSVLQQA
                     SPTVIPVITVDDAQLGTAPGSPRPAVVPTGEDIVGLFNSAASRAGGLLEAARYAGHAD
                     LYRAHYETLAALNKAANRSTTHDAYATARSAAQFLGKNLAAELAITPTDLAAYGIDGN
                     MRPDVAEIGKTLIVTAKAFQLRLTSSVVLPGLRDDPHDAFTNPTRLNATTAGLKRVLD
                     AFMADLGNRTDTVTGTKLSDDIVMTFEGDTPKAPLDNTNWLDNTPQNSNWVYVFGGGK
                     LKTGWFGGIAPGGQDGIVTGFDPTTGAATAYDGDTQAKAAVGAVAYAVTRGDIRAVQD
                     FTRIDISGLIV"
     gene            complement(5857..6465)
                     /locus_tag="VFV99_09240"
     CDS             complement(5857..6465)
                     /locus_tag="VFV99_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059532.1"
                     /translation="MKNALLLGVLLAACTTDAEIDDPSYTGHAQDPWVELECVQREGP
                     PRYASRVHGCPKMRVLTTGNVLASRGVNLAAADDLSAGKIYRTSAAALGAPNYAARIR
                     ENVDLGVATASKLFDIYVQAAPEIITNLPNRPECQKNGAPAQLFDSSNRCVAAGFSCL
                     MGVPATAQHLAICNETVKRAADVESGKRMAVALLAAAAHTCE"
     gene            complement(6468..7574)
                     /locus_tag="VFV99_09245"
     CDS             complement(6468..7574)
                     /locus_tag="VFV99_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059533.1"
                     /translation="MMRWPLAVVLFAACVPDPPPSDPHDDWDDKLAEREVDYNAALRI
                     AALRLTGELPTLVELDAIASAPNQKVAYEEQIQRYLGNPKFARQMYRYAQDMLKMGDD
                     PQLDTAAAFVAQVVINDRPYLDVLTATSGTCPSFNPATSQFTPGNCSNNAPATVGLLT
                     HPGVQKHYFSNLAFRRVRWLQETFACTKFPAEIAKTATNVGGTMPYTGVFPFDSIAGA
                     PTGRVDFKNTASVICANCHSNINHIAPLFAHFDADGRYTDGFAVPVPLPDNPPVEMRD
                     YLPNGEGLAWRFGVPISDMTSLGRAMAADPTIAECAIARVWDWAMGKNDIVDGGARVP
                     SATIEAQVAAFVAGGYSMKDAMYRVFTSDDFVRF"
     gene            complement(7571..8314)
                     /locus_tag="VFV99_09250"
     CDS             complement(7571..8314)
                     /locus_tag="VFV99_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059534.1"
                     /translation="MRPIVSIGRVDSLHGMRGVLLACALLSGCLDEAMSGEASLTARD
                     RWTQQAWPALSSCVGCHGSQPAIDFFAPGDADGAYTTVFEFQPPVIDVDAPASSLLLT
                     MGKHTGPALDPTSAAAVLAWLQAERDERVPDMGESVRVGPFLPSLGTQMTIDLPVGGA
                     KLRMTAEAGEIGLYVSKLTLSAGSGVKLAHPLFVSRPPKPVLDDIDRFADIDLTLDMG
                     KTLELGPAAFLAFDPNDYLTIHFKTLEAP"
     gene            8829..10670
                     /locus_tag="VFV99_09255"
     CDS             8829..10670
                     /locus_tag="VFV99_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059535.1"
                     /translation="MTMRKMMLAATFLFAACGTVQDNAGSGSDKGSSDSGSDGSGSDG
                     SGSDGSGTDAGMGSGSDGMDGGGMPTQCMTAEDCGGGNKACDVQTHECVTGVLTIDNT
                     AFVAEGPRYWTATSNPTLHGLYVGPSSAVIQVIVGNGQGTLATMTGTAWSLTLPANAI
                     GTTDTNVRVMMTDPSGGHVELMETLIVDNVAPRITLTTSTVHDESDDQIDFSSGEPVH
                     AHTGPNVTLTPTGCPVVHKYAYLTGTQDPVFGSQTAPNPIAFRFTIQDTKLQQGSMMY
                     RVRTSDGQTLLDWTALPPPITLDDYSIKLFSDNMAALRTRSGQFYFDVKARDWSGLDS
                     TATWCWNQKLLAPPLKVEALSPGEVFQWSLPSDSPVSSVIESEPPILKQRIVQYTNEA
                     IHLSWTPYIGAVQYTRNSVDDIVTETSLYPIGCPTGTTNPQCAIRNPPVEPADTPASG
                     VSTGLMSISAYEDATNAWVGVGNETLELDIPARAPNDPPKAYLIVVTRSVPELRPSGI
                     LYPDRARENQLVGLTFTGGTPTDLGYSCRDMKDTPIGRLCYQVDHYWRAQMLDSATVD
                     FGTITIAFKTQSAIQPLTAVPYLSAAASSSGPLVWNSGNDDLPGALH"
     gene            complement(10667..11428)
                     /locus_tag="VFV99_09260"
     CDS             complement(10667..11428)
                     /locus_tag="VFV99_09260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5059536.1"
                     /translation="MTWWQSFFDTDYLRLWSKFLTPERTANEVAQLVDLVDIRPNTRV
                     LDAPCGQGRISRGLAERGAIVLGVDQAPELLAAAERSRGDITTDHLRYLQHDLRNPLA
                     EHGFDVALNIFSSLGYGDEIDDLAILTTLAKALHAGGTLVVETNHRDNLVAKLARGDN
                     HPHTLPDGTTLREEIEFDAVRGRVESTWRWSGPNGDGSKRMSLRVYAVPELVRLVERA
                     GFAIKSVNLGLTTQPYKAEPPAMGGRLVIIGTRAA"
     gene            complement(11428..>11694)
                     /locus_tag="VFV99_09265"
     CDS             complement(11428..>11694)
                     /locus_tag="VFV99_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059537.1"
                     /translation="PAPPVPAAAAPAPAEAPRAKPATNAPPIEEIDEGWDMPDDDPTA
                     TSDAAQAAAAADAAMERPADIPSSSEMAGDGSTGGDGIDEPGWD"
BASE COUNT         1927 a         3788 c         3931 g         2048 t
ORIGIN      
        1 gacacatggt cggcgcggtg ctctcgctcg ccggcgtcgc cgtgctgttt ctcgatcggc
       61 tcgacgtctc gaccgagcaa gccgtcgggg tcgcgctcgt catcggctcg gtgctgctcg
      121 cgacgagcta ctcgatggtg atgaagcggc agaccacggg cggcctcgtc gcgaccacag
      181 tgtttctcgg tgtcaccgcg ctcgcactca gcgtcgtcgc gatcgtcgcc ggcgaaccga
      241 tcccatggcc gcctccactc gagccgacgc tcgcgctgct ctacttggcg atcgtcggct
      301 cggtgatcgc gttcttgctc tatttctgga tgctcaatcg cacgagcctc gtcgtgacga
      361 gcacgctcgt gttcgtgttt ccgctcgtcg cgctggttac tgacgccatg ttcgagcgcg
      421 agatcgccct cgggccgcgc gcgtatcttg gcgcagccat caccttgggc ggccttgcag
      481 tgacgctccg tcgaaaatga gcccaggtca gacggcagga gcatctgacg acgctagctt
      541 gaacgtgtct gctcggtaac aatccggtag atgtcggacg tcaagcgcga ccacccgtgg
      601 atcttccgta cgtacgcagg tcactcgagc gcgcgcgctt cgaacgagct gcatcgcaag
      661 aacctcgccg cgggccaaac gggtctgtcg atcgcgttcg atctgccgac gcaatgcggt
      721 tacgacgccg atcatccgct cgcgcgtccc gaggtcggca aggtcggtgt gccgatcgca
      781 tcgctcgacg acatgcacgc gctgttcgac ggcattccga tcgagcagat gaacacgtcg
      841 atgacaatca acggcaccgc gatgtggctg ctcgcgctct acgtcgcgct cgcgcgcgaa
      901 cgtggcgtgc ccgaggctgg tctgcgcggc acgacgcaga acgacatcgt caaggaatac
      961 ctcgcgcgcg gcacgtacat ctttccgccc gagccatcgc tcgatctgat cgccgaaacg
     1021 tacgagtact gcgtcgcgaa tctgccgcag tggaatccgt cgaacatctg cagctatcac
     1081 ctgcaggaag ccggcgcgac gccggtgcag gagctcgcgt tcgcactcgc ggatgcgatc
     1141 ggcgtgctcg atcgcgtgaa ggcgcgcggc cgcgtcgata gtgccgccat ggcgcaagcg
     1201 gtcggccgcg tctcgttctt cgtgaacgcg ggcatgcgtt tcgtcgaaga gatgtgcaag
     1261 atgcgcgcgt ttggcgagct gtgggacgac atctgcagca agcgctacgg cgtcaccgat
     1321 ccgaagctgc gccagttccg ctacggcgtg caggtcaact cgctcggcct caccgagcag
     1381 cagcccgaga acaatgcctg gcgcattctg atcgaagcgc tcggcgtcac actgtcgaag
     1441 gacgcgcgct gtcgagcgct ccagctgccg acgtggaacg aagcattgtc tctgccccgc
     1501 ccgtgggacc agcagtggtc gctgcgcctg caacagatcc tcgcgtacga gacggacctg
     1561 ctcgaatacg gcgatctgtt caccggcaat ccggttgtcg agcgcaaggt cgcggcgctg
     1621 tgcgatgcgg cgcgcaccga gctcgcgcag atcgacggca tgggcggcat tcgcggcgcg
     1681 atcgagagcg gctactgcaa gcgcgagctc gtgcggtcga tggcacaacg catggcgcgg
     1741 atcgagcgcg gcgagcagac ggtcgtcggc gtcaacaaat ggaccgaggg cctgcagtcg
     1801 ccgctcgtcg gcggtgatga cggcggtgtg ttcaaggtcg atccggcggc cgtcgacgcc
     1861 gcgttcgcat cgctcgaaca aacgcgcaag cgccgcgacg ccggcaacgt gaaggccgcg
     1921 cttgccgagc tcgaagcgaa ggcgcgcaaa ggcgagccga tcatggagac ctcgatccag
     1981 tgcgcgctcg cccgcgtcac gaccggcgaa tgggccggca cgctacgcaa ggtgtggggc
     2041 gaataccgcg cgcagaccgg cgtacagggc gcgcgcatcg ccgatggcga cggctggaat
     2101 gcgctacgcg cgcgcgtcga agcacacgtg gcgaagacgg gtcggcgacc gcgcctgctc
     2161 gtcggtaagc ccggcctcga cggccactcc aacggcgccg aggtgatcgc agtcgctgca
     2221 aaggatgccg gcttcgaggt gatctacgcg ggcattcgcc tcgagcccgc ggcgatcgcg
     2281 cagaccgcgg tgcaagaagc agtcgacgtc gtcggcctct cggtgctgtc gggttcgcac
     2341 gtcgagctcg cgaaggcggt gctcgaagag ctgcgcacgc gcggcggcag cgacgtcgcc
     2401 gtcgtgctcg gcggcacggt gccggcgtcg gatcacgcgt cgctgcgcga gctcggtgtg
     2461 cgccgcatct tcacgccgag tgactaccgg ctcatcgacg ttgtcggcgc gctactcgat
     2521 ctctgctgaa aaggggtctg accggtctga ccccatttct ggtctgacct atttccggca
     2581 gctgaagcgg aagtaggcgg cgttgccgca ctgcgagtca ccccacgttc cgttgtcgtc
     2641 catgaacgcg cactgctcgg catgatcgtt ctcgttgcca ttgacgtcgt tcggttggcc
     2701 ggccgcccag tgcgtatacg tcggcgtcag gccgtcactg ttccaggtcc acccggtggt
     2761 gagcgacgtc gccgtcgacg attgctcgaa gccgatccag ttgagctctc cctgaaatat
     2821 ctgctcgacg cagttctgat cggcctgcgt gcggatcggc gcgagcttgc cgccccacgc
     2881 cgtgcatgcg gtcaccgcgt ggtcctggtt cggtacgttc gcggtctgcg tgcaaccaac
     2941 ccagcacgcg ccgttgcagt tcatcgcgac agtcgtgccg acgcagatca gcccggtggt
     3001 gctgcacggc ggcggcggca gcgagctgtc gggcggcgcg tcgacgatga cgttcatggt
     3061 gtcgcccggc gcatcgacag cggcgagaac atcatccagc gtggatggcg catcgtctcg
     3121 cgtgaccgag gtgccgtgct cgaagccgca cgccgccact aacgcgatac agagacaccc
     3181 gagccgcaca gcctcattat gcgcgattgg ccgacggaat gggggccgac ctgttccgct
     3241 agttcttccg gcagctgaag cggtaggagt tgtcgtcgtc acacggtgtg tcatgccagt
     3301 caccgttggt gttcatcgtc gcgcactgct cggcgtgatc ggtctcggtg ccgtcggtgt
     3361 cgtttggctg gccggcggcc caatgcgtga aggtcggtgc gatgccgtcg ctgttccagg
     3421 tccagcccgt ggtcagcgag gtcgccgagc tcgattgctc gaagccgatc caatgactct
     3481 gccccgggaa cagcatctgt gcgacgcagt cttgatcgcc ctggtcgcgg atcggcgcga
     3541 gcttgccgcc ccagttcgca cacgacagca ccgccgtgcc ttggctcggc accgaaacgc
     3601 cgatctgcgt gcacttggcc cagcacgcgc cgttgcacat gagcgagctg gtgacgccgg
     3661 cgcacacgag ccccgagctc gagcattcgg gcgccgcgtc gatcgtgacg ttcgtcgtgt
     3721 cactcggcgc atcgagtagc ccatcacgcg attgcccggg tgcgtcgaag atcgacgtgc
     3781 cgtgctcgaa gctgcaggcc gccaaaagca tcacgcagca gagccgtcca agccgcacgg
     3841 ctttcattat gcgcgcgtca gcgctggcgg aggaactaat aggcgccggc cactgccggg
     3901 cggccatcgt attcgcatag atactcgtcg ctggtgtcac agctcgatac gtggaaccgc
     3961 ccaccatcgt cgaactgcga gcagtccgac gacgtcggct cgccgctgac ccacaccaga
     4021 tacggtgcag cctcgccggt cacgttgcga taggtgcctt cggtaatgcg atcggtcatg
     4081 ccggcccagt agtcgttgat cacgatgctc gtcgcgagca ggttgccctc agtggcgtcg
     4141 tcgatcgtca cgaggtgtgc accgacacca tccgcttcgc aggcgagctc gccgtcgagc
     4201 caggtcggca cttgcgtgac cgagaagcga tagcagctcg tgccgagttt catgtaggtc
     4261 gctgggcatt ggccggcatc gagcgcgagc atcggagcgt cggccgtcag ctgcgcgtcg
     4321 atggcgatct ctgcttcgct cggcgggacc gcgccgtcgt cgcgcgatgg tccgcggtcg
     4381 aagccaaacc gaccacagcc gatgagcacg agcaccaccg cgacgcgtcg cacacgtcca
     4441 tggtcgcacg acggccagtg aactagacga tcagtccgct gatatcgatg cgcgtgaagt
     4501 cctgcacggc gcggatgtcg ccgcgtgtga ccgcataggc gaccgcgccg accgccgcct
     4561 ttgcctgcgt atcgccgtcg taggcggttg ctgcgccggt cgtcggatcg aacccggtga
     4621 cgatgccatc ctggccgccg ggggcgatgc cgccgaacca accggtcttg agcttgccgc
     4681 cgccaaacac gtagacccag ttgctgttct gcggcgtgtt gtcgagccag ttcgtgttgt
     4741 cgagtggcgc cttcggcgtg tcgccttcga acgtcatcac gatgtcgtcg gagagcttcg
     4801 tgccggtcac ggtgtcggta cgattgccga gatcggccat gaatgcgtcg agcacacgct
     4861 tgaggccggc cgtcgtcgcg ttgagccgcg tcgggttcgt gaatgcgtcg tgcggatcgt
     4921 cgcgcaagcc cggcagtacg accgacgacg tcagccgcag ctggaacgcc ttcgccgtca
     4981 cgatcaacgt cttgccgatc tcggcgacgt ccggccgcat gttgccgtcg atgccgtagg
     5041 cggcgagatc ggtcggcgtg atcgcgagct cggcagcgag gttcttgccg aggaactgtg
     5101 cggcgctgcg tgcagtcgcg tacgcgtcgt gcgtcgtcga tcgattcgct gccttgttca
     5161 acgcagcgag cgtctcgtag tgcgcgcgat agaggtccgc gtgaccggcg taacgcgccg
     5221 cttcgagcag gccgcctgcg cgcgacgccg cgctgttgaa caggccgacg atgtcttcgc
     5281 cggtcggcac gactgctggc cgcggcgaac cgggcgcggt gccgagctga gcatcgtcga
     5341 cggtgatcac gggaatcact gtcgggctcg cctgctggag caccgagacg atcgcgaaca
     5401 gcgagttgcc attcaccgac cggacgatcg aattcgggtt cggcgtgtgc gcctcgttgc
     5461 tgcccgccat gaacgcggtc atctgctgat tcgcgggcag gttcgcgaac ggcgtgttcg
     5521 ggccgagcgt taacgcgccg ccggtgccct ggatcttctg cgtctgcgcc gctgcgaacg
     5581 gccacgtgct cgtgctgcca ccggcgaccg ctgcgaccgc attgtgtggc cacagcagcg
     5641 tgaaccacgc gatgccaccg ttgacgccgc gaatgtgcac cgagcgtttc gttggcgtgg
     5701 tagccgctgc ctgggcgacg ccgcgaccgg cgtgtcgctc gaggatctcg agcaagcgcg
     5761 tctgcgagac gccgagtgca gcgccggcgg caacgctcca tttgatcagc gcgcgtcgcg
     5821 tgagctctcg tttgtgatcg cgagacatgg tgcctcctac tcgcaggtgt gagccgcggc
     5881 tgcgagcagc gcgacggcca tgcgcttgcc gctctcgacg tcggcagcgc gtttgacggt
     5941 ttcgttgcag atcgcgaggt gctgcgcggt ggccggaacg cccatcaagc agctgaagcc
     6001 tgctgccacg cagcgattcg agctgtcgaa cagctgggca ggggcgccgt tcttctggca
     6061 ctcgggacgg ttcggcaggt tcgtgatgat ctcgggcgcc gcctgcacgt agatgtcgaa
     6121 cagcttcgac gccgttgcga caccgaggtc gacgttctcg cgaatccgtg ccgcgtagtt
     6181 cggtgcaccg agggccgccg cgctcgtgcg gtagatcttg cccgccgaca gatcgtcagc
     6241 cgccgcgagg ttcacgccgc gcgatgcgag cacgttgccg gtggtgagca cgcgcatctt
     6301 cgggcagccg tggacgcgcg acgcgtaccg cggtggccct tcgcgttgca cgcactcgag
     6361 ctcgacccat ggatcttggg catgtcccgt gtacgacggg tcgtcgattt ctgcgtcggt
     6421 cgtgcacgca gcgagcagga cgcccagtag caatgcgttc ttcatggtta gaacctcacg
     6481 aagtcgtcgc tggtgaacac gcgatacatc gcgtccttca tcgagtagcc gccggcgaca
     6541 aacgcggcga cctgcgcttc gattgttgcg ctcggtacgc gagcaccgcc gtcgacgatg
     6601 tcgttcttgc ccatcgccca gtcccacacg cgtgcgatcg cgcattcggc gatcgtcgga
     6661 tccgcggcca tcgcgcggcc gagcgacgtc atgtcactga tcggcacgcc gaagcgccac
     6721 gcgagccctt cgccgttcgg taggtagtcg cgcatctcga cgggcgggtt atcaggcagc
     6781 ggcaccggca ctgcgaagcc gtcggtgtag cggccgtctg catcgaagtg cgcgaacagc
     6841 ggagcgatgt ggttgatgtt cgagtggcag ttcgcgcaga tcaccgacgc cgtattcttg
     6901 aagtccacgc ggccggtcgg tgcgcccgcg atgctgtcga acgggaacac gcccgtgtac
     6961 ggcatcgtgc cgccgacgtt ggtcgcggtc ttcgcgatct cggccgggaa cttcgtgcac
     7021 gcgaacgtct cttgcagcca gcgcacgcgc ctgaacgcga ggttcgagaa gtagtgcttc
     7081 tgcacgccag gatgcgtgag cagaccgacg gtcgccggcg cgttgttcga gcagttgccc
     7141 ggcgtgaact ggctcgtcgc cggattgaag ctcgggcagg taccgctcgt cgcggtgagc
     7201 acgtcgaggt aggggcgatc gttgatcacg acctgcgcga cgaacgccgc cgcggtatcg
     7261 agctgcggat cgtcgcccat cttcagcatg tcctgcgcgt agcggtacat ctggcgcgcg
     7321 aacttcgggt tgccgaggta gcgctggatc tgctcctcgt acgcgacctt ctggttgggc
     7381 gcagaagcga tcgcatcgag ctcgacgagc gtcggcagct caccggtcag gcgcagcgcg
     7441 gcgatccgca acgccgcgtt gtagtcgacc tcgcgctctg cgagcttgtc atcccaatcg
     7501 tcgtgcgggt cgctcggcgg cgggtcgggc acgcacgccg caaacaacac gacagcaagt
     7561 ggccagcgca tcatggtgcc tccagcgtct tgaagtggat cgtcaggtag tcgttcgggt
     7621 cgaacgcgag gaacgctgcc gggcctagct ccagcgtctt ccccatatcg agcgtcagat
     7681 cgatgtcggc aaagcgatcg atgtcgtcga gcaccggctt gggcggccgc gacacgaaca
     7741 gtggatgcgc gagcttcacg ccgctgccgg cgctgagcgt cagcttcgag acgtagaggc
     7801 cgatctcgcc agcctcggcc gtcattctca gcttcgcccc accgaccggt aggtcgatcg
     7861 tcatctgcgt gccgagcgag ggcagaaacg gcccgacccg gaccgactcg cccatgtcag
     7921 gcacgcgctc gtcgcgttcg gcctgcagcc aggcgagcac ggcggccgcc gacgtggggt
     7981 cgagggcagg gccggtatgc ttgcccatcg tcagcaagag gctcgaggcg ggcgcatcga
     8041 catcgatgac gggcggctgg aactcgaaca ccgtcgtgta cgcgccgtcg gcgtcaccgg
     8101 gtgcgaagaa gtcgatcgcc ggctgcgagc catgacagcc gacgcagctc gataacgcgg
     8161 gccacgcttg ttgcgtccag cggtcgcgag ctgtcagtga cgcttcacca gacatggcct
     8221 cgtcgaggca gccggagagc agcgcgcacg cgagcagaac ccctcgcatg ccgtgcaggc
     8281 tgtcgacgcg gccgatcgaa acgatcgggc gcatgccgag cgcatcagca acgcgcgtgc
     8341 cgtgagggag tgttcgaacg ctgcgcatgc gatcgagagc gcaagctcgc gaccgtgcac
     8401 aatcagaacg ctctcagcgc gacgaattca tcgcgctgtc aaaatcttct taactcgcgt
     8461 gcaacaacca tcagtttttc ggcgggaccg ttttccccac gccggtgatg tcaacaaaca
     8521 ccatttgcgt cagtgatcac gcacgcaccg cttgcgagga agaaatgcgc atcaccaccg
     8581 tagccgtggt tctcgacgag aaagccagct gagacgccca gcgattcgag cacttgcaac
     8641 tttcttcgca accgcttctc ggcgtgtcga tacgcgcggc aaggaggaca ttccttccca
     8701 catgactgaa gacggcccgg ctcgcccccg ggccgtcgcc ttttcggcgc ccgcgcggcg
     8761 ccgcttttgt ccgcagatcg gacgaaaccg aaagggaggt gtgtcgatag gcgcgcccat
     8821 aggaggcaat gacaatgcgc aagatgatgc tggcggcgac gttcctgttc gccgcgtgtg
     8881 gaacggttca agacaacgcc ggcagcggct cggacaaggg cagcagtgac tccggctcgg
     8941 atggctcggg ctcggatgga tcgggctcgg atggctccgg cacggatgcg ggcatgggct
     9001 ctggttcgga tggcatggac ggcggcggta tgccgacgca gtgcatgacc gccgaggact
     9061 gcggcggcgg caacaaggcg tgtgacgtgc agacgcacga gtgcgtgacc ggcgtcctca
     9121 cgatcgacaa cacggccttc gtggcggagg gcccgcggta ctggacggcg acgagcaatc
     9181 cgaccctgca cggtctttat gttggaccga gctcggcagt gattcaggtg atcgttggta
     9241 acggccaggg cacgctcgcc acgatgaccg gtacggcctg gagtctcacg ctgcctgcga
     9301 acgcgatcgg tacgaccgac acgaacgtgc gcgtgatgat gacggacccg agcggcgggc
     9361 acgtcgagct gatggaaacg ctgatcgtcg acaacgtcgc gccgcgcatc acgctgacga
     9421 cgagcacggt gcatgacgag agcgatgatc agatcgactt cagcagtggc gaaccggtgc
     9481 acgcgcacac ggggccgaac gtcacgctga ccccgaccgg ttgcccggtc gtccacaagt
     9541 acgcgtacct gaccggcacg caggatccgg tattcggctc gcagactgcg ccgaatccga
     9601 tcgcgtttcg gttcacgatc caggacacca agctgcagca gggctcgatg atgtatcgcg
     9661 tccgcacgag tgatggccag acgctgctcg attggaccgc gctgccaccg cccatcacgc
     9721 tcgacgacta ctcgatcaaa ctgttcagcg acaacatggc cgcgctgcgc acgcgctcgg
     9781 ggcagttcta tttcgacgtg aaagcgcgcg actggtcggg gctcgactcg acggcgacct
     9841 ggtgctggaa ccaaaagctt ctcgcgccgc cgctgaaggt ggaggcgctc tcgccgggcg
     9901 aggtgttcca gtggagcctg ccaagtgatt cgccggtgtc gagcgtcatt gaaagcgaac
     9961 caccgatctt gaaacagcgc atcgtgcagt acacgaacga ggcgattcac ctgtcgtgga
    10021 cgccgtacat cggcgccgtg cagtacacgc gcaactcggt cgacgacatt gtcacggaga
    10081 cgagcctgta tccgatcggt tgcccgaccg ggacgaccaa tccgcagtgc gcgatccgga
    10141 atccgccggt cgaaccggcc gacactccgg cgagcggcgt cagcacgggc ctgatgagca
    10201 tctcggcgta cgaggacgcg acgaatgctt gggttggtgt gggcaacgag acgctggagc
    10261 tggatattcc ggcgcgcgct ccgaacgatc cgccgaaggc gtacctcatt gtcgttactc
    10321 gcagcgtgcc ggagcttcgg ccatccggca tcctctaccc ggatcgggcg cgcgagaacc
    10381 agcttgttgg cctgacgttc accggaggca cgccgacgga cctcggctac tcgtgtcgcg
    10441 acatgaagga caccccgatc ggcaggctct gctatcaggt cgatcactac tggcgtgcgc
    10501 agatgctcga ctcagcgacg gtcgacttcg gcacgatcac gatcgcattc aagacgcaga
    10561 gcgcgatcca gccgcttacc gctgtgccct atctctcggc ggcggcaagt tcgagcggtc
    10621 cgctcgtgtg gaattccggc aacgacgatt tgccgggcgc tctgcactag gcagcgcgcg
    10681 taccgatgat cacgagccgc ccgcccatcg cgggcggttc tgctttgtac ggctgcgtcg
    10741 tgagcccgag gttcacgctc ttgatcgcaa agccggcgcg ctcgacgagg cgcaccagct
    10801 cgggcaccgc gtagacgcgc aacgacattc gcttcgagcc gtcgccgttc gggccggacc
    10861 agcgccacgt cgattcgacg cggccgcgca cggcatcgaa ctcgatctcc tcgcggagcg
    10921 tcgtgccatc gggcagcgtg tgcgggtggt tgtcgccgcg cgcgagcttc gcgaccaagt
    10981 tgtcgcgatg gttcgtctcg acgacgagcg tgccaccggc gtgtagcgcc tttgcgagcg
    11041 tagtgaggat cgcgaggtca tcgatctcgt ccccgtagcc gagcgacgag aagatgttga
    11101 gtgcgacgtc gaagccgtgc tcggcgagcg ggttgcggag gtcgtgctgg aggtagcgga
    11161 gatggtcggt ggtgatgtcg ccacgcgagc gctcggcggc agcgagcagc tcgggcgctt
    11221 gatcgacgcc gagcacgatc gcgccacgct cggcgagccc gcgcgagatc cgaccctggc
    11281 cgcacggcgc atcgagcacg cgcgtgttcg gccggatgtc gacgaggtcg acgagctgtg
    11341 cgacttcgtt tgccgtgcgc tcgggcgtga ggaacttcga ccacaaccta aggtagtcag
    11401 tgtcgaagaa cgactgccac caggtcatca gtcccagccg ggctcgtcga tgccatcacc
    11461 gccggtcgag ccgtcaccgg ccatctcgga cgacgacgga atgtccgcgg gacgctccat
    11521 cgcagcgtcg gcagcggcag ccgcttgcgc agcgtcgctc gtcgcggtcg gatcgtcgtc
    11581 gggcatatcc cagccttcgt cgatctcttc gatcggtggc gcgttcgtcg cgggcttcgc
    11641 gcgaggcgcc tcggcagggg cgggcgcggc agcggccggt accggcggcg ccgg
//
LOCUS       DASUFX010000236         6323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_35288,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000236 DASUFX010000000
VERSION     DASUFX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35288"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..1035)
                     /locus_tag="VFV99_09270"
     CDS             complement(355..1035)
                     /locus_tag="VFV99_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059538.1"
                     /translation="MRSTLFVVLLCSSPVFARPVQPTPPRSSPPHAARGLPAPAPQPP
                     APALPKLTIADEDIPDECADLATLADSPSKNQALSARISLAACLVDEKSRALVLCDCE
                     QSVNEINAILDPSLAVLDDVVAAGDPAMQVLARYTQGSILAGFAQRMLSTVPPPVDAT
                     EASLALRDTRVTMLAPLVQPWLMRAQGAFTQVDKLARENPKLGKNPAVVAAVRSSREK
                     LSQGVANR"
     gene            1187..4012
                     /locus_tag="VFV99_09275"
     CDS             1187..4012
                     /locus_tag="VFV99_09275"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX5059539.1"
                     /translation="MATPSGIPVLGDLPPGSHIGQFYWDQHDLLETLVPFFATGLRQN
                     ERCIWVCSQPLCAAEARSALAKVVYNLAELERKGQIAIVDHDAWYVQRGELTTGRVIQ
                     GWLDAERDALQSGYRGLRINGNLMWVPPEQWSQLADYEAQAHTAFRDRAIVALCSYPL
                     SKCGAHQIVDVLHNHGASLVRNGSGWGLVHGATAALASLDTEHFLARAEQSHSVEFYR
                     DRFPIDRVAARLHDALDRGVGAAALVMREHGNALRSELLRRDVDVNAAIGRGQLAILD
                     ADAVFEAAWTRPGVRLQVVIEAVRTPISAIIERYGSCAAYGELVDLFARNGDRDAAIA
                     LERWWNDQLLTLPIDLACGYSLASFDDASTVAQFKTVCNEHRDVGIDGAVTASEADRL
                     RVELAQMTNALAREMARRQVIEAAYATARKAREHLVLLNRLTTALGEVTSRAQFADLV
                     RDLVVRTLDAAAIVLVEVDSREPLVCEGFGAESLCQAALVPTTRARWSNRMPEPAGNT
                     ELRAYAVLPVMVGSRRVATLALGFAHDRELSAPYRGLTDDVARQLALALDRAISYERL
                     EHERKRAESGSRAKDEFLAMLGHELRNPLSPILTATQLMRLRGEDVFEKERTVIERQC
                     RHMIRLVDDLLDVSRITRGKVELRRRPIEICEVVAQAVEQVSPAMEERAHRLTIDVPK
                     SGLVVHADPARLAQVIGNLLTNAAKYTPHGGAITVAVRATGSAVAIAVRDTGIGIDPK
                     LLPQVFGLFIQGRQGIDRAAGGLGLGLAIAKTLVELHGGTIHARSQGAGQGSEFVVEL
                     PRYANTRPAQRSNNSGAFVLAGIRPFRVLVVDDNEDAAFLFAEALRKLGHKVDVANDG
                     PSALAVARQNPPEIAFLDIGLPVMDGYELGRRLREIEGAPPKLVAVTGYGHSSDRERS
                     LDAGFALHLVKPVDLTAIQDALAKLCT"
     gene            4027..4311
                     /locus_tag="VFV99_09280"
     CDS             4027..4311
                     /locus_tag="VFV99_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059540.1"
                     /translation="MPSVPLLALAWVAIWLWRWAKPSPVVFGLAIVPASVATTLTLLW
                     WPEATEQQITYAMFAAEIAVLVPFALMMRQQVVRLVQFVFPRARVVRKRY"
     gene            complement(4308..5114)
                     /locus_tag="VFV99_09285"
     CDS             complement(4308..5114)
                     /locus_tag="VFV99_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059541.1"
                     /translation="MTQDVARVGDYRIERELGNDATGVIYEATHLVLPRRAALKVCHD
                     RSCAVQLMREACVLEALAHPGVPRVYECGVLPDKRPWVARELVDGISIGERLGRGSMA
                     LSDIVVMVRTISEILAHAHARGVVHDRLGNTAVIVTPQRPFPYCVRGWTSVVVQDSER
                     GADPSADVHALGVLAYRALIGELPERPSAQENATAAPLELTALIDQMLADDPRWRPTA
                     TEVRDRAAWLAQTLELVPDARPRWTPPHGLGERASTQAEPELDVVVRIRS"
     gene            complement(5234..>6323)
                     /locus_tag="VFV99_09290"
     CDS             complement(5234..>6323)
                     /locus_tag="VFV99_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX5059542.1"
                     /translation="ACGWFGINAWVGGAALQVFFVSIFPKWATLLGTAGYPTAGFQNT
                     QWISFALFWGLNIFIIFRGMELLRRVERFAAPFVLVMTAALLVWMLKEAGGLGPILER
                     SSFEPSAKHPTLMGVMIPALTGTIAFWSTLSLNMPDFTRFGRSQKEQAVGQTVALPST
                     MTVFAAMGIMITSATWIVYKEPIDNPILLASKFDSRLVVAIATFTIVIATLAVNIAAN
                     VVSPANDFANAFPKKINFQRGGLITGLIGIAMVPWELLKDPERYIGGWLGGYGAALGS
                     IGGVLIVDYWILRKTELDLRSLYVTDGRYRYTSGWNVPAVVATLLGAGIALLGAFWEP
                     MKPIYDWSWFVGFGIAGGLYYALMRHQS"
BASE COUNT         1072 a         2114 c         2114 g         1023 t
ORIGIN      
        1 gagcgcgcgc cgaccgcctc gggcgagatc aagaaacgcg gctagcgagt gcgttgacgt
       61 tgccgctggc ggttggttgc tctctggcgc gaccatccgc acatgctcga cgtcgccgcc
      121 cgagggcgcc gccgccccag agcgaacgcc aagatcccaa ggtcaccgag aacgtcaagg
      181 aactttcaga tcgcgttctg ctcgagcgat cacgcgtgcg gagcgcgatc aagaaccctt
      241 tcttcttggc gttcttggcg gcttggctcg ccttggcgtt agatctccgg acgcgactcc
      301 tgtcgcgctc gccaagaacg cgcaaccacg acgatgctcg cttgcttgca aacgctagcg
      361 gttcgcgacg ccctgcgaga gcttctcgcg gctcgagcgc actgccgcaa cgaccgcggg
      421 gttcttgccg agcttcgggt tctcgcgcgc gagcttgtcg acttgcgtga acgcaccctg
      481 ggcgcgcatc aaccacggct gaacgagtgg cgccagcatc gtcacccgcg tgtcgcgcag
      541 ggcgagcgat gcttccgtcg cgtccacggg cggcggcacg gtcgagagca tgcgttgcgc
      601 gaagccggcc aggatggagc cctgtgtgta acgcgcgagg acctgcatcg ccgggtcacc
      661 ggccgcgacg acatcgtcga gcaccgcgag actcggatcg aggatcgcgt tgatctcgtt
      721 caccgactgc tcgcagtcgc acaggacgag agctctggat ttctcgtcga cgagacacgc
      781 ggcgagcgag atgcgcgccg agagcgcctg gttcttgctc ggcgagtcgg caagcgtcgc
      841 gaggtcagca cactcgtcgg ggatgtcctc gtcggcgatc gtcagcttcg ggagtgctgg
      901 cgctggcggc tgcggcgccg gcgctggaag cccgcgcgct gcatgaggcg ggctggaccg
      961 aggcggcgtc ggttgtacgg gccgagcgaa tacgggcgac gagcacaaca agacaacgaa
     1021 gagagtcgaa cgcatgacac tcaaaagtgc agtggtcatg cccgcccaat ccggcgcgca
     1081 ttcgagcaac accgtgctcc ggcgccacgg gtttgtgccc ctatcgctag acgattcgct
     1141 gtgcttcaca cgctcgcttt ttgcacatgg cggcagagct atagctatgg cgacgcctag
     1201 tggaattcca gtactcggcg acctgccccc gggttcacat atcgggcagt tctactggga
     1261 ccagcacgat ctcctcgaaa cgctcgttcc attcttcgcc accgggctac ggcaaaacga
     1321 gcgctgcatc tgggtctgtt cgcagccgct gtgcgccgca gaagcgcgca gcgcgctggc
     1381 gaaggtggtc tacaacctcg ccgagctcga gcggaaaggt cagatcgcga tcgtcgatca
     1441 cgacgcgtgg tacgtgcagc gcggcgagct gacgacgggc cgtgtgattc agggttggct
     1501 cgacgcggag cgcgatgcgc tccagtctgg ctaccgtggg ctccgcatca atggaaatct
     1561 gatgtgggtg ccgcccgagc agtggtcgca gctcgccgac tacgaagcgc aggcccacac
     1621 agcgttccgc gaccgcgcga tcgtcgcgtt gtgtagctat ccgctcagca agtgcggcgc
     1681 gcaccagatt gtcgacgtgc tgcacaacca cggcgcctcg ctcgtgcgca acggcagcgg
     1741 ctggggcctc gttcacggcg cgacggccgc gctggcatcc ctcgacaccg agcacttcct
     1801 cgcccgcgcc gagcagagtc attcggtcga gttttatcgt gaccggtttc ccatcgatcg
     1861 agttgccgcg cgtctgcacg acgcgctcga tcgcggcgtt ggcgccgcag cactcgtgat
     1921 gcgcgagcac ggcaatgctc tgcgctccga gctgctgcgg cgcgacgtcg atgtcaacgc
     1981 agcgatcggc cgcggccagc tcgccatcct cgatgcagat gccgtgttcg aagcggcgtg
     2041 gacgcgcccc ggcgtccggc tccaggtcgt gatcgaggcg gtgcgcacgc cgatcagcgc
     2101 gatcatcgag cgctacggtt cgtgcgccgc gtacggcgag ctcgtcgacc tgttcgcacg
     2161 caatggcgat cgcgatgctg cgatcgcgct cgagcgctgg tggaatgacc agctgctgac
     2221 actgccgatc gatcttgcat gcggctattc gctcgcgagc ttcgacgatg cgagcaccgt
     2281 cgcgcagttc aagaccgtgt gtaacgagca tcgcgacgtc ggcatcgatg gcgcggtgac
     2341 ggcgtccgag gcggatcggc tgcgcgtcga gctcgcgcag atgacgaacg cgctcgcccg
     2401 cgagatggcg cgacgccagg tgatcgaggc cgcctacgcg acggcacgca aggcacgcga
     2461 gcacctcgtg ctgctcaacc ggctgaccac cgcgctcggc gaagtcacca gccgcgcgca
     2521 gtttgccgac ctcgtgcgcg acctcgtcgt gcgcacgctc gatgcagccg cgatcgtgct
     2581 cgtcgaggtc gatagtcgcg agccgctcgt ctgcgaggga ttcggcgccg aatcgctgtg
     2641 ccaagcggcg ctcgtgccga caacgcgcgc gcgctggtcg aatcgtatgc ccgagcccgc
     2701 cggcaacacc gagctgcgcg cgtacgcggt gctcccggtg atggtcggct cgcggcgcgt
     2761 ggcgacgctc gcgctcggct ttgcccacga ccgcgagctc agcgcaccgt atcgcgggct
     2821 gaccgacgac gtcgcccgcc agcttgcact cgcactcgat cgcgcgattt cgtatgaacg
     2881 cctcgagcac gagcgcaagc gcgccgaatc cggcagccgc gcgaaggacg agttcctcgc
     2941 gatgctcggt cacgagctac gcaacccgct gtcaccgatc ttgaccgcga cgcagctgat
     3001 gcggctgcgc ggtgaggacg tgttcgaaaa agagcgcacc gtgatcgagc gccagtgccg
     3061 gcacatgatc cggctcgtcg acgatctgct cgacgtctcg cggatcacgc ggggcaaggt
     3121 cgagctgcgc cggcggccga tcgaaatctg cgaggtcgtc gcgcaggctg tcgaacaggt
     3181 cagcccggcg atggaggagc gcgcgcatcg gctcacgatc gacgtgccga aaagcggcct
     3241 cgtcgtgcac gcggatccgg cacggctcgc gcaagtgatc ggcaacctcc tgacgaacgc
     3301 ggcgaagtac acgccgcacg gcggtgcgat cacggtcgca gtgcgcgcca ccggcagcgc
     3361 cgtcgcgatc gcagtccgcg acaccggcat cggtatcgat ccgaagctgc tgccgcaggt
     3421 gttcgggctg ttcatccagg gacgtcaggg catcgatcgc gccgccggcg gactcgggct
     3481 cggcctggcg atcgcgaaga cgctcgtcga gctgcacggc ggcacgatcc acgcgcgcag
     3541 tcagggcgcg ggtcagggca gcgagtttgt cgtcgagctt ccgcggtacg cgaacacgcg
     3601 gccggcgcag cgcagcaaca acagcggcgc gttcgtgctc gccgggatcc gaccgttccg
     3661 cgtactcgtc gtcgacgaca acgaggacgc cgcgttcctg ttcgccgaag cgctgcgcaa
     3721 gctcggccac aaggtcgatg tcgcaaacga tggtccgagc gcgcttgccg tggcgcgcca
     3781 gaacccgccg gagatcgcgt tcctcgacat cgggcttcct gtcatggacg gttatgagct
     3841 cggccggcgg ctgcgcgaga tcgaaggcgc gccgccaaag ctcgtcgcgg taacgggtta
     3901 cggtcattca tcggatcggg agcgctcgct cgacgcgggt ttcgcgctgc acctcgtcaa
     3961 acccgtcgac ctcaccgcca ttcaagacgc gctcgcgaag ctctgcacct gagctacgct
     4021 agaagcatgc cgtccgtgcc actactcgcg ctggcttggg ttgcgatctg gctgtggcgc
     4081 tgggcgaagc cgtcgccggt ggtgttcggg ctcgcgatcg tgcccgcgtc agtcgcgacg
     4141 acgctcacgc tcctgtggtg gccggaagcg acagaacaac agatcacgta cgcgatgttc
     4201 gcggcggaga tcgccgtgct cgtcccgttc gcgctgatga tgcgccagca ggtcgtgcgg
     4261 ctcgtgcagt tcgtgtttcc ccgcgcgcgc gttgtccgca aacgctacta agagcgaatg
     4321 cgaacgacga cgtcgagctc gggctcggcc tgcgtcgacg cgcgctcgcc gagaccatgt
     4381 ggcggcgtcc accgcggccg cgcatcgggc acgagctcga gcgtctgcgc gagccacgcg
     4441 gcacgatctc gcacctcggt ggcggtcggt cgccaacgcg gatcgtcggc gagcatctga
     4501 tcgatcagcg ccgtgagctc gagcggggcg gccgtcgcgt tctcttgcgc cgacggtctc
     4561 tccggcagct cgccgatcaa cgcgcgatac gccagcacgc cgagtgcgtg tacatcggcg
     4621 ctcggatcgg ctccgcgctc cgagtcctgc acgacgaccg acgtccagcc gcgcacgcaa
     4681 tacgggaacg gccgctgcgg cgtgacgatc acggccgtgt tgcccagccg atcgtggacg
     4741 acaccgcgcg catgcgcgtg cgcgagaatc tcggagatcg tgcgcaccat cacgacgatg
     4801 tccgagagcg ccatcgagcc gcgcccgagt cgctcgccga tcgagatgcc gtcgacgagc
     4861 tcgcgcgcga cccagggtcg cttgtcgggc aagacgccgc actcgtagac gcgtggaacg
     4921 ccgggatggg caagtgcctc gagcacgcac gcctcgcgca tcaactgcac ggcgcacgat
     4981 ctgtcgtggc agaccttgag ggccgcgcgg cgcggcagga cgaggtgggt ggcttcgtag
     5041 atgacgccgg tggcgtcgtt acctaactcg cgctcgattc ggtaatcccc gacccgagct
     5101 acatcttgag tcatcaggcc gccagttggg tgcagtaccc gtgcctcgca tttgcgtgcg
     5161 gatgtccgcg aatcgtcgac gccgcaaccg gttgcgcgtg gtctgccgac cacataacga
     5221 cgtttggcta gcctcagctc tgatgtcgca taagcgcgta gtagaggccg cccgcgatgc
     5281 cgaacccgac gaaccacgac cagtcataga tcggcttcat cggctcccag aacgcgccaa
     5341 gcagtgcaat gcctgcaccg aggagcgtcg ccacgacggc gggaacgttc cagccgctcg
     5401 tgtagcgata gcggccgtcg gtgacgtaga gcgagcgcag atcgagctcg gtcttgcgca
     5461 ggatccaata gtcgacgatg agcacgccgc cgatggaacc gagcgctgcg ccgtagccac
     5521 cgagccagcc gccgatgtag cgctcgggat ccttgagcag ctcccacggc accatcgcga
     5581 tgccgatgag gccggtgatg agaccgccgc gctggaagtt gatcttcttc gggaacgcgt
     5641 tcgcgaagtc gttcgccggt gacacgacgt tcgctgcaat gttcaccgcg agcgtcgcga
     5701 tcacgatcgt gaacgtcgcg atcgcgacga cgaggcggct gtcgaacttc gatgcgagca
     5761 ggatcgggtt gtcgatcggc tccttgtaga caatccacgt cgcgctcgtg atcatgatgc
     5821 ccatcgcggc gaacacggtc atcgtcgacg gcagcgcgac ggtctggccg acggcctgct
     5881 ctttctgcga gcggccgaag cgcgtgaagt cgggcatgtt gagcgacagc gtcgaccaga
     5941 atgcgatcgt gccagtcagc gctggaatca tcacgcccat cagcgtcgga tgcttcgcac
     6001 tcggctcgaa cgagctgcgc tcgaggatcg gaccgaggcc gccggcctcc ttcagcatcc
     6061 acacgagcaa cgccgccgtc atcacgagca cgaacggcgc cgcgaaccgc tcgacgcgac
     6121 ggagcagctc catgccgcgg aagatgatga agatgttgag gccccagaac agcgcgaacg
     6181 agatccactg cgtgttctga aacccggccg tcggatagcc cgcggtgccg aggagcgtcg
     6241 cccacttcgg aaagatcgac acgaagaaga cctgcagcgc cgcgccaccg acccacgcgt
     6301 tgatgccaaa ccaaccgcac gcg
//
LOCUS       DASUFX010000237         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1841528, whole genome shotgun sequence.
ACCESSION   DASUFX010000237 DASUFX010000000
VERSION     DASUFX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841528"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1132)
                     /locus_tag="VFV99_09295"
     CDS             complement(107..1132)
                     /locus_tag="VFV99_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059543.1"
                     /translation="MRAQAVLLMALLLPLIAEADGERLGVVVREGVHDATIVARLRGQ
                     LADLEGVAMESYPTGDSIEPTLDGQLAAAERIGAANDARVVVWFVARGKKLAVAIATP
                     RDHRLFVREIPPAAESAMAEAAAIAVRSAVRSIALGGTIGVEVKAAPVEERAEPIPVP
                     VPIAKGAAPVAIDAALGWQVALDGGAQHGAHALVQRTTITRGPWGASLALELGVPLEW
                     RAGTDVALDVSRSGALLGGERRVAGGVAFGVGAGALVYHRSTSTAPVGLAATPSKSTV
                     AFASAVEVAWHARIAGRIGVVACAGVDLVLGAPEAAVSRAAMIEVVDTIRPVQPRLSL
                     AVEVGSW"
     gene            complement(1148..2293)
                     /locus_tag="VFV99_09300"
     CDS             complement(1148..2293)
                     /locus_tag="VFV99_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059544.1"
                     /translation="MNKHLVAIFTLALVGCVAEIGSPPGGGGSGGDDDGSGSGSGSGT
                     GGGSGGTAPAGLIFSAYKDTSINMDWNTNVISTMVPGTRTALVDDMTAHGAKTITLAF
                     ATGECGSENWGGVPGEAMASTNVPKLTAANIDYILATGGAAGAFTCGSDANMMAFLAR
                     WASPHLIGIDYDIEAGQSEEVIGQLIQRIVAAHAAYPGLRWSLTLATLANNDGTPTAV
                     SLGAAATSSFNIKGDQTLMAVKSKLGFDGTAATWPSYVTVNLMTMDYGGPSPGVCVVS
                     SGLCDMGQSAIQAAYNLHDHWGVPYSNIELTPMIGGNDVQNEHFTLQDTDEMSAFAIE
                     KKLAGVHYWSYDRDVDCPQGSASPTCNSLGGIGPHGFLARFIGNGMN"
     gene            complement(2397..2723)
                     /locus_tag="VFV99_09305"
     CDS             complement(2397..2723)
                     /locus_tag="VFV99_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059545.1"
                     /translation="MSEDVRLQELVQVLPPRIWYLTSNGTDMWCRRPYGFLFSTGQAA
                     ETFAKAMGTGEELFAVGLDAGAFINDAMLAGLRNSAVTRLFIDPAIDPESGDVHGKIL
                     RLAPLT"
     gene            2761..3246
                     /locus_tag="VFV99_09310"
     CDS             2761..3246
                     /locus_tag="VFV99_09310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5059546.1"
                     /translation="MIRLALACALLLVACGPGSKGGPTMNNRINSPAPVAQGSDVASG
                     DILAREPVANNAEVKHILISWKDLDSATDQRAKKRTKEEAEALVKSLLGQIKAGTEFE
                     TLMKEYSEDPGSASTGRGYPVSPDAQLVIEFKQLGLRLNVGEVGVVQSDFGFHIMKRI
                     K"
BASE COUNT          586 a         1148 c         1098 g          578 t
ORIGIN      
        1 acgaggttac gcacgcgcgg caacagctca cgacacagcg cctgcgcggc catgcgatcg
       61 ccgcgcgctg cggccgcgat gcgcggatcg atcgctgcgg ccggtgtcac cacgaaccga
      121 cctccaccgc gagcgacaag cgtggctgca ccgggcgaat cgtgtcgacg acctcgatca
      181 tcgcagcgcg cgagactgca gcctcgggcg cgccgagcac caggtcgacg ccggcgcacg
      241 cgacgacgcc aatgcggccg gcgatccgcg cgtgccatgc aacctcgacc gcacttgcga
      301 atgcgacggt cgacttgctc ggcgtcgctg cgagtccgac cggcgccgtc gacgtcgagc
      361 gatgatagac aagcgcacca gcgccgacac cgaacgcgac gccaccggcg actcggcgct
      421 cgccaccgag aagcgcgccg ctgcgcgaaa catcgagcgc gacgtcggtg cccgcgcgcc
      481 actcgagtgg cacgccaagc tcgagagcga gcgatgctcc ccatggccct cgcgtgatcg
      541 tcgtgcgttg cacgagcgcg tgtgcgccgt gctgtgcgcc gccatcgagc gcgacttgcc
      601 agccgagcgc agcgtcgatc gcgaccggcg ccgcgccctt tgcaatcgga accggcacgg
      661 ggatcggctc cgctcgctcc tcgactggcg cggccttcac ctcgacaccg atcgtgccgc
      721 cgagcgcgat cgaacggact gcgctgcgca cggcgatcgc tgccgcttct gccatcgccg
      781 attcggcggc cggaggaatt tcgcgtacga acaagcgatg gtcgcgaggt gttgcgatcg
      841 cgacggcgag cttcttgccg cgtgcgacga accacacgac gacgcgcgcg tcgttcgctg
      901 caccgatccg ctccgccgct gcgagctggc cgtcgagagt cggctcgatc gagtcgccgg
      961 tcggatagct ctccatcgcg acgccctcga ggtccgcgag ctggccgcgc aaccgcgcca
     1021 cgatcgtggc gtcgtgcaca ccctcgcgca cgacgacgcc gaggcgctcg ccatcagctt
     1081 ccgcgatgag cggtagcagc aaggccatca gcaacaccgc ctgggctcgc acgcaggcat
     1141 cagcgtatca gttcatcccg ttgccgatga agcgcgcgag aaagccgtgg ggaccgatgc
     1201 caccgaggga gttacacgtc ggcgacgccg aaccctgcgg gcagtcgacg tcgcggtcgt
     1261 acgaccaata gtgaacgcca gccagtttct tctcgatagc aaatgcgctc atctcgtcgg
     1321 tgtcttgcag cgtgaagtgc tcgttttgaa cgtcgttgcc gccgatcatc ggcgtcagct
     1381 cgatgttcga gtacggcacg ccccagtgat cgtgaaggtt gtacgcagcc tggatcgccg
     1441 attgccccat gtcgcacagc ccgctgctga cgacgcacac gcccgggctc ggcccgccgt
     1501 aatccattgt catgaggttt acggtgacgt agctcggcca cgttgccgcg gttccgtcga
     1561 agccgagctt cgacttgacg gccatcagcg tctgatcgcc tttgatgttg aaactgctcg
     1621 tcgcggcggc accgagcgat acggcggtcg gggttccgtc gttgttcgcg agcgtcgcga
     1681 gcgtgagact ccagcgcaac ccaggatatg cggcgtgcgc ggcgacgatc cgctggatga
     1741 gctgaccgat cacttcttcg gattgaccgg cttcgatgtc gtaatcgatt ccgatcagat
     1801 gaggcgaggc ccaccgcgcg aggaacgcca tcatgttggc atcgctgcca cacgtgaacg
     1861 cacccgctgc gccgccggtc gcgaggatgt agtcgatgtt cgccgcagtc aacttcggca
     1921 cgttcgtcga cgccatcgct tcaccgggca cgccgcccca gttttcgctg ccgcactcgc
     1981 cggtcgcgaa cgcgagcgtg atcgtcttcg cgccgtgtgc cgtcatgtca tcgacgagcg
     2041 ccgtgcgcgt gccaggcacc atcgtcgaga tgacgttggt gttccagtcc atgttgatgc
     2101 tcgtgtcttt gtacgcactg aagatcagac cggcgggcgc ggtgccaccg ctgccgccgc
     2161 ccgtgcccga tccggagccg gatccggatc catcatcgtc accgcccgaa ccgccaccgc
     2221 ccggcggact tcctatttca gcgacgcaac cgaccagtgc gagcgtgaaa atcgcgacga
     2281 gatgcttgtt catgtctcga tagagagacc gcgcccaagg atcgggtcat gggaatcttt
     2341 cgcagcgatc cccgatgcat gcgcgaggct gaccggtcgg tggtttcccg atcgagctag
     2401 gtcagcggcg caaggcgcaa gatcttgccg tgaacgtcgc cgctctcggg atcgatcgcg
     2461 ggatcgatga acagccgcgt gaccgcgctg ttgcgtagac cggcgagcat ggcgtcgttg
     2521 atgaacgcgc ctgcgtcgag gcccaccgcg aacagctcct cacccgtgcc catcgccttc
     2581 gcgaatgtct cggccgcctg gcccgtcgag aacagaaaac cgtacgggcg gcggcaccac
     2641 atgtccgtgc cgttcgaggt caggtaccag atgcgaggcg gcagtacctg tacgagctcc
     2701 tgcaaacgaa cgtcctcgga catccgggtc ttatacctcg acgcgtgata gcttgcgcgc
     2761 atgattcgcc tcgcgctcgc ctgtgcgctg ttgctcgtcg cctgcggtcc cggctcgaag
     2821 ggcggaccga cgatgaacaa tcgcatcaac agcccggcgc cggtcgcgca aggctccgac
     2881 gtcgcctcgg gtgacatcct cgcgcgcgag ccagtcgcga acaacgcaga ggtcaagcac
     2941 atcctgatct cgtggaagga tctcgatagc gcaacggatc agcgcgcgaa gaagcgcacg
     3001 aaggaggagg cggaggcgct cgtgaaatcg ctgctcgggc agatcaaggc cggcaccgaa
     3061 ttcgaaacgt tgatgaagga gtactcggag gatccgggca gcgcgtcgac gggtcgcggc
     3121 tatccggtat cgccggatgc gcagctcgtg atcgagttca agcaactcgg gttgcggctc
     3181 aatgttggtg aagtcggtgt cgtgcagtcc gactttggct ttcacatcat gaagcggatc
     3241 aaatgatcgc gaccaccgca gcggtttcgc tcggcctgct cgtcggcgcg cgccacgcgt
     3301 tcgagcccga tcacctcgca gcggtgtcca cgctcgtcac gacctcgcgc aacgccaagg
     3361 gcgccgcgtt gctcggcctg ctgtggggta ttggtcatac gattgcgttg
//
LOCUS       DASUFX010000238         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_174627,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000238 DASUFX010000000
VERSION     DASUFX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174627"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFV99_09315"
     CDS             <1..472
                     /locus_tag="VFV99_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059547.1"
                     /translation="LAELTDDAATIDAADLNKTKLELCAQTAQRLGLTSIRRHVCDLL
                     DANAPLADAYDLIVLDAPCSGLGVLRRHPDAKWRLQPADVPRLADLQRQLLDAVVPRL
                     VPGGTLVYSVCTFTRAEGPDQVAALVARTGLKLVSEQRSWPPDADAFYIARLTR"
     gene            complement(750..1364)
                     /locus_tag="VFV99_09320"
     CDS             complement(750..1364)
                     /locus_tag="VFV99_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059548.1"
                     /translation="MRTALLVVVVLAACSKKAEQKKQEPTPAPEAAKPAETKPAEAPK
                     PPDDKPAPLPTPAPKLDCAALLTADDLSKACGKKLEIEATRYEGQGALMVCNRTIKDP
                     AKHMSAQFGVSSFNDSAAIDAWIKLTKDQSKESKFTDVTGIGDGGYEKVHEVKGLKST
                     DIDLAVRKGNFLAHFGFMIGSSEKPVCTIDQLKEVAKLVVSRMP"
     gene            1397..1573
                     /locus_tag="VFV99_09325"
     CDS             1397..1573
                     /locus_tag="VFV99_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059549.1"
                     /translation="MDEFVQKMVDKVGIDKSTAMKVIDFLKEHADDAVEYLQKSGLKD
                     KLPGGLGDKLGKLF"
     gene            complement(1579..>3964)
                     /locus_tag="VFV99_09330"
     CDS             complement(1579..>3964)
                     /locus_tag="VFV99_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059550.1"
                     /translation="VFASVEHALAGNSMVVRQAVLEEWLRAAEGIRAFDDKQVDKLVR
                     MTVTLAEAGDDTDDRRAANRALFYTPHAGARRALMDAVKNASTAKNDELRYNLYFGLS
                     HVDHPDVLPFLVERMFSEREEYWALLEALETKVDAAANVRILGTLVERAADPSAVHAA
                     TVYADVLLHKKQSPRLLIDLARVVIGWTPATNDDARRMRYLFEQAMAGALDLMRPDDA
                     RTFLARARELPDSPYSDYLVKDRDQKTPALLANAEMKKRIAALESGKLDAQIAEARAT
                     AESARAAGKPIAADDARLGTLAGCKVSGRFFEDRDTHVVWFFDEVGALHVYDGYAIGV
                     PSGQVTGTGGQGIPWNGMAAFVAGHPMIDERVLFLDTKTSRAREIIRLGDRLLVHDGN
                     GKDYWDHVSIPAIGLKFATTAEARHMFGRLVANPPKGTKRVDTWYVGGAGAVRRKYYY
                     PLPGAGYNEDGEARIAVLGRQIDGTLDDKCPPLGREFPTGDAAIAAMEAWETRVFSAG
                     GRITHLWIDGEATRREDTLLSAFLDQRYRDDNQTAAWHLKSLGEMYGAIAEAGLTPLV
                     PDIQVRLGPPATDAELAAYQALVGEPLPDPLLQLWRQVGGGGFTSTETSARFLSPAEL
                     VSGRSGLRKALEEWINTRLKGSAKAHKLAMLDDLDVIAVRDDVPLIIFDTKQRQRDGR
                     CFASADSDWWESALGWQIATDINVLLHSQLQRRLGDVFRLKLGQRVGAGVRRTQLTKG
                     TDKKWEAIVDDTQLLVRSTGKTFFKPTIKTFASAQAAAKAFDSAVADAKKKGFR"
BASE COUNT          608 a         1331 c         1314 g          711 t
ORIGIN      
        1 cctcgccgag ctcaccgacg acgcggcgac gatcgacgca gccgatctca acaagaccaa
       61 gctcgagctg tgtgcgcaga ccgcgcagcg gctcggcctc acatcgattc gccgccacgt
      121 ctgcgatcta ctcgacgcga acgcgccgct cgccgatgca tacgatctca tcgtgctcga
      181 cgcgccgtgc tcgggcctcg gcgtgctgcg ccggcatccc gacgcgaaat ggcgcctgca
      241 acccgccgac gtgccgcggc tcgccgacct gcagcgccag ctgctcgacg cggtcgtgcc
      301 gcgcctcgtg cccggtggca cgctcgtcta ctcggtgtgc acgttcacgc gcgccgaggg
      361 gccggatcaa gtcgcagcgc tcgtcgcgcg caccggcctg aagctcgtta gcgagcagcg
      421 gtcgtggccg cccgatgccg acgcgttcta catcgcgcgg ctcacgcgtt agacctgcga
      481 gtggagaagc acgacgttgg ggcgcactgg gcgcttctgg agcgtcgact ggcgccttcg
      541 ccgccgcacg acgtgttcca gactaacgcc aagaagccaa gacgccgagg ccgccaccag
      601 aaggagggtc ttcgacgcgg tctgcagacg cggtcgtgcg agcataacgc gatcccgaaa
      661 cgcttcttct ggcgttcttg gcgtcttggg gccttggcgt tcctctgggc gccgccaagc
      721 ggctccctcg acgccatctc agcacactgc tacggcatgc ggctgacgac gagcttcgcg
      781 acttccttca gctgatcaat cgtgcagacc ggcttttcgc tcgagccgat catgaagccg
      841 aagtgcgcga ggaagttccc cttgcgcacc gcaaggtcga tgtcggtgct cttgaggcct
      901 ttgacctcgt gcaccttctc gtagccgccg tcgccgatcc cagtgacatc ggtgaacttc
      961 gattccttgc tctggtcttt ggtcagcttg atccacgcat cgatcgccgc gctgtcgttg
     1021 aagctgctga cgccgaactg cgcgctcatg tgcttcgcgg ggtccttgat cgtgcgattg
     1081 cagaccatga gggcgccctg accttcgtag cgcgtcgcct cgatctcgag cttcttgccg
     1141 catgccttcg acaggtcgtc ggcagtcagc agtgcggcgc aatcgagctt cggcgcgggc
     1201 gtgggcagcg gcgccggttt gtcgtcgggc ggctttggcg cttcggcggg cttcgtctcc
     1261 gccggcttcg cggcttccgg agcgggagtc ggttcttgct tcttctgctc cgccttcttc
     1321 gagcaggcag ccaacacgac aacaacaagc agtgcggtgc gcatggcgcg gactctatct
     1381 ttggtaaggt ccgccaatgg acgaattcgt gcagaagatg gtcgacaagg tcgggatcga
     1441 taaatccacc gcgatgaagg tgatcgactt cctcaaggag cacgccgacg acgccgtcga
     1501 gtacctgcag aagtccggcc tcaaggacaa gctgccgggc ggcctcggcg acaagctcgg
     1561 caagctgttc tgatcgcgct agcgaaagcc cttcttcttg gcgtcggcaa cggcgctatc
     1621 gaacgccttc gccgctgcct gagccgacgc gaaggtcttg atcgtcggct tgaagaacgt
     1681 cttgcccgtc gagcgcacga gtaactgcgt gtcgtcgacg atcgcttccc attttttgtc
     1741 agtgcccttc gtgagctgcg tgcgccgcac gccagcgccg acgcgctgac cgagcttgag
     1801 ccggaacacg tcgccgagcc ggcgctggag ctggctatgg agcagcacgt tgatgtcggt
     1861 cgcgatctgc cagccgagcg cgctctccca ccagtcgctg tcggcggacg cgaagcagcg
     1921 accatcgcgc tgccgttgct tggtgtcgaa gatgatcagc ggcacgtcat cacgcaccgc
     1981 gatcacgtcg agatcgtcga gcatcgcgag cttgtgcgcc ttcgccgaac ccttgagccg
     2041 ggtgttgatc cactcctcga gcgccttgcg cagtccgctg cgaccggaga cgagctcggc
     2101 cggcgacaag aaccgcgcgc tggtctccgt cgacgtgaag ccgccgccgc cgacctgacg
     2161 ccagagctgg agcagaggat cgggcagcgg ctcgccgacg agcgcctggt aggcggcgag
     2221 ctctgcgtcc gtcgccggtg gaccgagccg gacctggatg tcgggcacga gtggcgtgag
     2281 gccagcctcg gcgatcgcgc cgtacatctc gccgaggctc ttcagatgcc aggcagcggt
     2341 ctggttgtcg tcgcgatagc gctgatcgag gaacgccgag agcaacgtgt cttcgcgacg
     2401 ggtcgcttcg ccgtcgatcc acagatgcgt gatgcggccg cctgccgaga acacgcgcgt
     2461 ctcccacgct tccattgccg cgatcgccgc gtcgccagtc ggaaactcgc gcccgagtgg
     2521 cgggcatttg tcgtcgagag tgccgtcgat ctggcggccg agtaccgcga tccgcgcctc
     2581 gccgtcttcg ttgtagccgg cgcccggcaa cggatagtag tacttgcggc gaaccgcgcc
     2641 cgcgccgccg acataccacg tgtcgacgcg cttggtgccc ttcggcggat tcgcgacgag
     2701 gcggccgaac atgtggcgcg cctcggcagt ggtcgcgaac ttgagcccga tggccgggat
     2761 cgagacgtgg tcccagtagt ccttgccgtt gccgtcgtgg acgagcagtc gatcgccgag
     2821 ccggatgatc tcgcgggcgc gcgacgtctt cgtgtcgagg aacagcacgc gctcgtcgat
     2881 cattggatgc ccggcgacaa acgcggccat gccgttccac gggatgcctt ggccgccggt
     2941 gccggtgacc tgaccgctcg gcacgccgat cgcgtagccg tcgtagacat ggagcgcgcc
     3001 gacctcgtcg aagaaccaca cgacgtgcgt gtcgcggtcc tcgaagaagc gccccgacac
     3061 cttgcagccg gcgagcgtgc cgagccgcgc gtcgtcagcg gcgatcggct taccggccgc
     3121 gcgtgcggat tctgcggtgg cacgcgcctc cgcgatctgc gcgtcgagct tgccgctctc
     3181 gagcgcggcg atgcgcttct tcatctcggc gttcgcgagc agggccggcg tcttctggtc
     3241 gcgatccttg accagataat cggagtaggg cgaatcgggt agctcgcgcg cgcgtgcgag
     3301 gaacgtgcgc gcatcgtcgg ggcgcatcag gtcgagcgca ccagccatcg cctgctcgaa
     3361 gaggtagcgc atccggcgcg catcgtcgtt cgtcgccggt gtccagccga tcacgacgcg
     3421 tgcgagatcg atgagcaaac gcggcgactg cttcttgtgc agcagcacgt cggcgtagac
     3481 ggtcgcggcg tggacggcgc tcggatcggc cgcgcgctcg acgagcgtgc cgaggatccg
     3541 gacgttcgcg gccgcatcga ccttggtctc gagcgcctcg agcagcgccc agtactcttc
     3601 gcgctcgctg aacatccgct cgacgagaaa cggcaggacg tcggggtgat cgacgtgcga
     3661 gagcccgaag tagaggttgt agcgaagctc gtcgttcttc gcagtgctcg cgttcttgac
     3721 cgcatccatg agcgcgcgcc gcgcgccggc gtgtggcgtg tagaacagcg cgcgattcgc
     3781 ggcgcggcga tcgtcggtgt catcgccagc ttcggcgagc gtcaccgtca tgcgcacgag
     3841 cttgtcgact tgcttgtcgt cgaatgcgcg gatgccctcg gccgcccgca gccactcttc
     3901 gagcaccgcc tggcgcacga ccatgctgtt gccggccagc gcgtgctcga ccgatgcgaa
     3961 cacc
//
LOCUS       DASUFX010000239         7960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1424709, whole genome shotgun sequence.
ACCESSION   DASUFX010000239 DASUFX010000000
VERSION     DASUFX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424709"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VFV99_09335"
     CDS             complement(<1..1535)
                     /locus_tag="VFV99_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059551.1"
                     /translation="MRLALGLALLLASSTSIAKPHEGRLRERVIGDAMPARARPAFAQ
                     LAQQGWRATWDRDTGVPARMWGGVVAAPGAVGDAAIAQRGAEQFLAAHLELLAPGAQL
                     GDLVVVANQVDDGIRTVGFAQHYRGVPVIGGQLGFVFAHDHLFVIGSSALPNVVATLV
                     GTTKAILPIARAGAITYHAVDIRDVGTMRVYIGSDGREVARESRVMTATGTLQYNAGV
                     RWAASTRDDMPAPAANITVNGSPVTTSATGTFTWTGTTAATVAPGLTGTFVKIVNQAG
                     GGTATTTLTVQPGGTGVWNLADDEFGDAQLSTYIYANQAKARARIVNPSVTTWLDGQL
                     DFYVNENGSCNAYSTGDDVHLFKKDATCENSGRIADIVYHEFGHSLHNHSVIAGMGKF
                     ESHLSEGLADFFAANLTEDPTIGHGFFLTDAPLRDIDPPGIERVYPLDFDFDPHISGL
                     IISGTLWDLRKALVRQLGMAAGVARAEKIFTGVMQRADDISTTFTAALIADDDDGNLG
                     NNTP"
     gene            1623..2417
                     /locus_tag="VFV99_09340"
     CDS             1623..2417
                     /locus_tag="VFV99_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5059552.1"
                     /translation="MRYALVVLAACSFDHGVDGTSDGPPANLSDVRPTDDGHATVDSA
                     IVDSANGQPTCTDGMQNGDELDVDCGGTTCGPCANIFTTDANTLALFELNGTLADSSG
                     NNRNATLIGGTFVTTSWGQGFAVPGQSTQGFQWNTYAAMIAHPYTIEMVVTAQSVSCW
                     KKLFGPSDSADAGWYFCGGFSSYPSNAIGPTFTTNQRHYIAIVSTSSNGIRVYADGAS
                     IGTSNNFTAPGGAAIFFRDDTNTGRSEALDGVVDAVRISKVARTAT"
     gene            complement(2453..5158)
                     /locus_tag="VFV99_09345"
     CDS             complement(2453..5158)
                     /locus_tag="VFV99_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5059553.1"
                     /translation="MRVAVAVAVVLASSQVQAGGSGFVVAGARQPSISGFATRAPARD
                     TFAAHGWHAIYDRDTGVAAFAWGTPIAAQGAMKDAAIAEGIARTTLAEHLALLAPGAT
                     VDDFVLVANRVDGNLRTIGFAQMSHGMPVLGGQLGFVFGNDRLFAISSQALPDVRVAL
                     PRKRATDATKLARAEAWLARDTGMRVQAHTTGEHVIVPIVFGAGDIAYHVVDIVDVSA
                     LSGGERWHVYVAADGSPVARKSLVMKDSSATLAFNVGVRYGTGTRADTPAPTTSLTVD
                     GTMTMTAADGTFTWPTTNAATIVPEAGGPHVRVTSVFVPPPTTLTAQPGSTVVWNRSA
                     DQYGDAILSAYIYASTAKTRARVVNPSVIPWLNGILDVYVNGTQTYCNAYSTGDDIHF
                     FIGNSVCDNSARIADIVYHEFGHSLHKHSVIDGMGDFEVALSEGLGDFYAALITEDSG
                     LARGFHFTNAPDREIDPIGVEKSYPQDVAADPHETGLIISGALWDLRKQLVAELGATA
                     GTAVIERIFAGVMMRAPKIPGAYMAALIADDNDANLANGTPHICQINTAFAPHGLRVD
                     LHMTTIGPATLTGLALDVPVADTVDCQRVMSMTVTWQSSTGASGSFALAPQSPTTMWS
                     GTFPDQPASSVLSYSIVATLADGSTRALPDNAADPMYQRFIGETKPIWCTNFDQDPRW
                     RASATSGVSAWQWDRPRGGLDPAAAFTGSHVLGNVLVGDGLYASGEHASIDTPEIDLE
                     GFSTVHLQYWRWLTTQDGTHDEATISALHDTGGPTELWHNLAMAGIDHVDREWRFHDI
                     DLGSFATSDPVHLRWDLESDGAVELGGWTIDDVCVVTADPSVCGDSEISGAEECDDGN
                     RTDGDGCSKLCRDEGGCCSASPNPLGPLVLATLLGLRLRRRRRMI"
     gene            complement(5367..6515)
                     /gene="metK"
                     /locus_tag="VFV99_09350"
                     /pseudo
     CDS             complement(5367..6515)
                     /gene="metK"
                     /locus_tag="VFV99_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            6583..7230
                     /locus_tag="VFV99_09355"
     CDS             6583..7230
                     /locus_tag="VFV99_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059554.1"
                     /translation="MRIVLLAGIVTLFACGGHPPPPKRGVVEKDLGSWKFRRFQPVLD
                     VEVWVEGNKAQAYTASYVADEAEKTGHVEDKDVVNVFVTRYEKPEGVLRETVKLVRRL
                     AAEGGYQVDEGKVGGVRALTINGHGESWVMWAAKMHVVKVGGRGRETVPEGVVESYGN
                     RYPSEIPGGALEGPLPAGPDNPVQKEEKEPYDAKNPKPDLEKYDPKKTKLPDKKK"
     gene            7227..>7960
                     /locus_tag="VFV99_09360"
     CDS             7227..>7960
                     /locus_tag="VFV99_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059555.1"
                     /translation="MTERERLARIFGIEPLRPVGALVMAAIMGAIPWTLMWVGSGSLV
                     VWMSAIFVDEQRKIRAGLGEVNGWGFPVSGYREWLLAREPAFDVELRRDVAIDLVEHS
                     LAAVDATIVVERRSERVVRVVMRRVEVRASEEMGTFLAGDRKRLGEVRDRVLAPLHAD
                     VGIAAMRMGDREGLGALVASPRSPMDGESAFRDQAKVAPPDLQSLVHAGASQLTPPRE
                     ARALDVRLDRLLYATGQQPSSGGAMAL"
BASE COUNT         1369 a         2516 c         2697 g         1378 t
ORIGIN      
        1 ggcgtgttgt tgccgaggtt gccgtcatcg tcgtcggcga tcagcgccgc cgtgaacgtc
       61 gtgctgatgt cgtcggcgcg ctgcatcacg ccggtgaaga tcttctcggc gcgcgcgacg
      121 cccgctgcca taccgagctg acggacgagc gctttgcgca gatcccacag cgtgcccgaa
      181 atgatgaggc cgctgatgtg tggatcgaaa tcgaagtcga gcgggtacac gcgctcgatg
      241 ccgggcggat cgatgtcacg cagcggtgca tcggtgagga agaagccgtg cccgatcgtc
      301 ggatcttcgg tgaggttggc ggcgaagaaa tcggcgaggc cctccgagag atgactctcg
      361 aacttgccca tgcctgcgat caccgagtgg ttgtgcagtg agtgaccaaa ctcgtgatag
      421 acgatgtcgg cgatgcggcc gctgttctcg caggtcgcat ctttcttgaa caggtgaacg
      481 tcatcgccgg tcgagtacgc gttacagctg ccgttctcgt tgacgtagaa gtcgagctga
      541 ccgtcgagcc aggtcgtcac gctcgggttg acgatgcgag cgcgcgcctt ggcctggttc
      601 gcatagatgt acgttgacaa ttgcgcatcg ccgaattcgt cgtcggcgag attccacacg
      661 ccggtaccgc cgggctgcac ggtgagggtc gtcgtcgcgg tgccaccgcc ggcctgattg
      721 acgatcttca cgaacgtgcc ggtaagcccg ggcgcgaccg tcgccgcggt cgtgccggtc
      781 cacgtgaacg tgccagtcgc cgacgtcgtc accggcgagc cgttgacggt gatgttcgct
      841 gcaggcgccg gcatgtcgtc acgcgtgctc gctgcccagc gcacgccggc gttgtattgc
      901 aacgtgccgg tcgcggtcat cacgcggctc tcgcgcgcga cctcgcggcc atcgctaccg
      961 atgtacacgc gcatcgtgcc gacgtcgcga atatcgacgg cgtgataggt gatcgcgccc
     1021 gcgcgcgcga tcggcaggat cgctttcgtc gtgcctacca gcgtcgcaac gacgttcggc
     1081 aatgccgagg agccgatcac gaacaagtga tcgtgtgcga agacgaaccc gagctgcccg
     1141 ccgatcaccg gcacgccgcg gtagtgctgt gcgaagccga cggtgcgaat gccatcgtcg
     1201 acctggttcg cgacgacgac gagatcgccg agctgcgcac ccggcgcgag cagctcgaga
     1261 tgtgcggcga gaaactgctc cgccccgcgc tgggcgatcg cagcgtcgcc gaccgcaccc
     1321 ggcgccgcga ccacgccgcc ccacatgcgc gcaggcacgc cggtatcgcg atcccacgtc
     1381 gcacgccaac cttgttgcgc gagctgcgca aacgctggcc gcgcgcgcgc cggcatcgca
     1441 tcgccgatca cgcgctcgcg taggcgtccc tcatgcggct tcgcgatgct cgtcgagctc
     1501 gcgagcaaca gggcaagacc tagcgccaag cgcacggcca accatggcac gagttcgcga
     1561 gtttgcgcgc ggcgtcgatc acgcagccac cgttccaaga ggccctttga taccctggat
     1621 cggtgcgcta cgcgctcgtc gtcctggcgg cttgctcgtt cgaccacggc gtcgatggga
     1681 cgtcggatgg cccgcctgcg aacctgtccg atgttcgccc gacggacgac ggccacgcga
     1741 ccgtggattc ggcgatcgtc gacagcgcga acggccagcc gacctgtacc gacgggatgc
     1801 agaacggcga cgagctcgat gtcgattgcg gcggcacgac gtgcgggccg tgcgcgaaca
     1861 tcttcaccac cgacgcaaac acgctcgcgc tgttcgagct gaacggcacg ctcgcggatt
     1921 cgagcggcaa caatcgcaac gcgacgctga tcggcggcac gttcgtgacg acgtcgtggg
     1981 gccaaggctt cgcggtgccc ggccagtcaa cgcagggctt tcagtggaac acgtacgcgg
     2041 cgatgatcgc gcatccatac acgatcgaga tggtcgtgac tgcgcagagc gtgtcgtgct
     2101 ggaagaagtt gttcgggccg agcgatagcg cggacgccgg ctggtacttc tgcggcggct
     2161 tctcgagcta tccgtcgaac gcgatcggcc cgacgttcac gacgaaccag cggcactaca
     2221 tcgcgatcgt ctcgacatcg tcgaacggga tccgcgtcta cgccgacggc gcgagcatcg
     2281 gcacgtcgaa caacttcaca gcacccggcg gcgccgcgat cttcttccgc gacgacacga
     2341 acacgggccg cagcgaagca ctcgacggtg tcgtcgatgc cgtgcggatc tcgaaggtcg
     2401 cgcgcacggc gacctagatc caagccgtgc aagcgaagct agccgtgcag ccttagatca
     2461 tgcggcggcg tctgcggagg cggagaccga gcagcgtcgc gagtacgagc gggccgagcg
     2521 gattcggcga cgccgagcag cagccacctt cgtcgcggca caacttcgag cagccatcgc
     2581 catcggtccg attgccgtcg tcgcactcct ctgcgcccga aatctcgcta tcgccgcaga
     2641 cactcggatc ggcggtgacg acgcacacgt cgtcgatcgt ccaaccgccg agctcgacgg
     2701 cgccgtcgct ctcgaggtcc caccgcaggt gcactgggtc gctggttgcg aaactgccga
     2761 ggtcgatgtc gtggaagcgc cactcacgat cgacgtggtc gattccagcc atcgcgaggt
     2821 tgtgccagag ctcggtcggc cctccggtgt cgtgaagcgc ggagatcgtc gcctcgtcgt
     2881 gcgtgccgtc ctgcgtcgtc agccaacgcc agtactggag atgcaccgtc gagaacccct
     2941 ccagatcgat ctcgggtgta tcgatggacg cgtgctcgcc agaggcgtag aggccatcgc
     3001 cgacgagaac gttgccgagc acgtgggatc cggtaaacgc ggctgcgggg tcgaggccgc
     3061 cgcgcggtcg atcccattgc cacgcagaca cgcccgaggt ggcggatgcg cgccatcgcg
     3121 gatcttggtc gaagttcgtg caccagatcg gcttggtctc accgatgaac cgctgataca
     3181 tcgggtccgc cgcgttgtca ggcaatgcac gcgtcgagcc gtcggcgagc gtcgcgacaa
     3241 tcgaatagga caacacgctg ctggccggct gatcgggaaa cgtgcccgac cacatcgttg
     3301 tagggctctg cggtgcgagc gcgaacgagc ccgatgcgcc cgtgctcgac tgccacgtca
     3361 cggtcatcga catcacacgc tggcaatcaa cggtgtctgc gaccggcacg tcgagcgcaa
     3421 gcccggtgag cgtcgctgga ccgatcgtcg tcatgtgcaa atcgacccgc aacccatgcg
     3481 gcgcgaacgc cgtgttgatc tggcagatgt gtggcgtgcc gttcgcgagg ttcgcgtcgt
     3541 tgtcgtcagc gatcagggcc gccatgtaag caccgggaat cttcggtgcc cgcatcatca
     3601 cgccggcaaa gatgcgctcg atcactgcag tgcccgccgt agcgcccaac tcggcgacga
     3661 gctgtttgcg caaatcccac agcgcgccgc tgatgatcaa cccggtctcg tgcggatcgg
     3721 ctgcgacgtc ttgcggatac gatttctcga cgccgatcgg atcgatctcg cggtctggtg
     3781 cgttcgtgaa gtgaaagccg cgcgcaagcc cggaatcctc ggtgatcagc gcagcgtaaa
     3841 aatcgccaag gccttcggat agcgcaacct cgaagtcgcc catgccatcg atgacagagt
     3901 gcttgtgcag cgagtggccg aactcgtgat agacgatgtc ggcgatccgc gcgctgttat
     3961 cgcagaccga gttgccgatg aagaagtgga tgtcatcacc ggtcgagtac gcgttgcagt
     4021 acgtctgggt cccgttcaca tagacgtcga ggatcccgtt gagccacggg atgacgctcg
     4081 gattgacgac tcgggcgcgc gtcttcgcgg tgctcgcgta gatgtacgcc gacaggattg
     4141 cgtcgccgta ttggtccgcc gagcggttcc acaccaccgt actaccgggc tgcgccgtta
     4201 gcgtcgtcgg cggcgggaca aagacactgg tgacgcggac gtgtgggccg ccggcctcag
     4261 gcacgatcgt cgccgcgttc gttgtcggcc acgtgaacgt gccgtctgcg gcggtcattg
     4321 tcatcgtgcc gtctacggtg agcgaggtag tgggcgccgg tgtgtcggct cgcgtcccgg
     4381 ttccataacg gacaccgacg ttgaacgcga gcgttgccga ggagtccttc atcacgaggc
     4441 tcttgcgagc cactggcgag ccatcagcgg ccacatagac gtgccagcgc tcgccaccgc
     4501 tgagcgcgct gacgtcgacg atatcgacga cgtgatacgc gatgtcgccc gcgccgaata
     4561 cgatcggcac gatgacgtgc tcacccgtcg tgtgcgcctg cacgcgcatg ccggtatcac
     4621 gcgcgagcca ggcttcggcg cgcgccagct tcgtcgcgtc ggtcgcgcgc ttgcgcggta
     4681 gcgccacgcg gacgtcgggg agcgcctgcg agctgatcgc gaacaggcga tcattgccga
     4741 acacgaaccc gagctggccg ccgagcacgg gcatgccgtg cgacatctgt gcgaacccga
     4801 tcgtgcgcag gttcccgtcg acacgattcg caacgagcac gaagtcgtcg accgtcgcac
     4861 cgggcgcaag cagtgcgagg tgctccgcga gcgtggtgcg cgcaatgcct tcggcgatcg
     4921 cggcatcttt cattgcaccc tgcgccgcaa tcggcgtgcc ccacgcgaat gctgcgacgc
     4981 cggtatcgcg atcgtagatc gcgtgccagc catgcgccgc gaacgtatcg cgtgctgggg
     5041 cacgtgtcgc gaaaccggag atgctcggct gacgcgctcc ggcgacgacg aagccggagc
     5101 cacccgcctg cacctgcgag ctcgccaata cgaccgcaac cgccactgca actcgcatgt
     5161 gcaaaacgat cgcacgactt gcgcggaggc gaaaatgcaa acgcgcgacc caggtgggga
     5221 cgcgcgccca gtgcaacaaa atgcggcggt gtgtgtcgct atgcgcgggc tacggcgccg
     5281 gccttcgtct tgccagcggt cttcttcgac gacttggtgc cgttgccgtt cgacaccgag
     5341 tgaccgttgc cgctcttggc gtgctcgcgc agcttgtcga cgagcggcgt cgactcccac
     5401 gtgaactccg gcagctcgcg gccgaagtga ccgtacgccg cagtcgcctt gaagatcggg
     5461 cggcggaggt tgagctcctg gatcagcatg ccggggcgcg cgtcgaacgt ctcgagcacg
     5521 gccttcgtca gcttctcgtc ggcaacgacg ccagtgccaa acgtgtcgac gagcatcgac
     5581 acgggctcgg cgacgccgat cgcgtaggcg aactggactt cgcacttctt cgcgagcttc
     5641 gcggcgacga catgcttggc gatgtagcgc gcgtagtacg cggccgagcg atcgaccttg
     5701 ctcggatcct tgcccgagaa cgcgccacca ccgtgacggg cgtagccgcc gtaggtgtcg
     5761 acgatgatct tgcggccggt gaggccggcg tcgccgtgcg ggccaccgat cacgaagcga
     5821 ccggtcgggt tgacgtgaat cttcgttttg ttcgagagca gcttcgccgg gatgatcggt
     5881 ttgatcacgt gctcgcgcac cgcctcttcg atcgtcttca gcttgacctt gtcgtcatgc
     5941 tgggtcgaga cgacgatcgc ttcgatgccg acgggctggc cgtcttcgta gcgaaccgag
     6001 acctgggtct tgccgtcggg gcgcagccag tcgatgccct tgaccttctt cttgcgaacc
     6061 tcggcgagct tgaacgcgag ctgatgcgcg agctggatcg gcagcggcat cagttccttg
     6121 gtctcgtcgc acgcgtagcc gaacatcagg ccctgatcgc cggcgccttg ctttgccgga
     6181 tcggtgccgc ggtcgacacc ctgtgcgatg tcgggcgact gcttcgccac cgcgaccatc
     6241 acgccgcacg aatcggcatc gaagcccatg tccgagctgg tgaaaccgat ctcgcggatc
     6301 gcgtcgcgcg cgattttgtt gtagtcgagg tgggctttcg tcgtgatctc gccgagcagc
     6361 atcacgaagc cggtcttcgt cgcggtctcg cacgcgacgc gactgttggg atcctccgcg
     6421 aggcacgcgt cgagcacggc gtcggagacg gcgtcgcaga gcttgtccgg gtgaccttcg
     6481 gtgactgatt cagacgagaa caggtagcta gccatgggtt tggcgggact attcaggttc
     6541 aggcgtccgc tgtcaacgga ggaacgaggt accatagcgg agatgcgaat cgtccttctc
     6601 gcagggatcg tgacgctttt tgcctgcggc ggtcatccac ccccaccgaa gcgcggcgtc
     6661 gtcgaaaagg atctcggatc gtggaagttc aggcggttcc agcctgtgct cgacgtcgaa
     6721 gtctgggtcg agggcaataa agcgcaggcc tatacggcga gctacgtcgc cgacgaggcg
     6781 gaaaagacgg gccacgtcga ggacaaagac gtcgtgaacg tttttgttac gcgctacgaa
     6841 aaacccgagg gcgtgctgcg cgagaccgtg aagctcgtgc ggcgattggc ggcagaaggc
     6901 ggctaccagg tcgacgaggg caaggtcggc ggtgtgcgtg cgctcacgat caatggccat
     6961 ggcgagtcat gggtgatgtg ggcagcgaag atgcacgtcg tgaaggtcgg tgggcgtggt
     7021 cgcgagacgg ttcccgaggg cgttgtcgag agctacggca accgctaccc gtcggagatt
     7081 cccggcggcg cgctcgaggg gccgctgccg gcgggtcccg acaatccggt gcagaaggaa
     7141 gagaaggagc cgtacgacgc gaagaatccg aaaccggatc tcgagaagta cgacccgaag
     7201 aagacgaagc tgcccgacaa gaagaaatga cggagcgcga gcggctcgcc aggatcttcg
     7261 gcatcgagcc gctgcgtccc gtgggagcgc tcgtgatggc ggcgatcatg ggggcgattc
     7321 catggacgct gatgtgggtc ggcagtggtt cgctcgtcgt gtggatgagc gcgatcttcg
     7381 tcgacgagca gcgcaagatc cgtgcgggcc ttggcgaggt aaatggttgg ggcttcccgg
     7441 tcagcggcta tcgcgaatgg ctgctcgcgc gcgagccggc gttcgatgtg gagctgcgcc
     7501 gcgacgtcgc gatcgatctg gtcgaacatt cgctcgccgc cgtcgacgcg acgattgtcg
     7561 tcgagcgacg tagcgagcgc gtcgttcggg tggtcatgcg gcgcgtcgag gtacgagcga
     7621 gcgaggagat gggaacgttt ctcgccggag accgcaagcg cctcggtgaa gtgcgcgatc
     7681 gcgtgctcgc gccgctgcac gcggacgtcg ggatcgcggc gatgcggatg ggtgatcgcg
     7741 aggggctcgg tgcgctcgtc gcgtcgccgc gtagtccaat ggatggtgag agcgcgtttc
     7801 gcgatcaagc gaaggtcgcg ccgccggatc tgcagagcct cgtgcacgcc ggggcgtcgc
     7861 agctgactcc gccacgcgaa gcgcgcgcac tcgacgtgcg actggacaga ctgctctatg
     7921 caaccggtca gcagccgtcg agcggcggcg cgatggcgct
//
LOCUS       DASUFX010000240         7625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1286872, whole genome shotgun sequence.
ACCESSION   DASUFX010000240 DASUFX010000000
VERSION     DASUFX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286872"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..530)
                     /locus_tag="VFV99_09365"
     CDS             complement(150..530)
                     /locus_tag="VFV99_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059556.1"
                     /translation="MTRILVVEDDEDLRAVVLHGLHTDGRTLLEADDGLAALTLIESQ
                     GLPDLILLDMNMPGMNGWQFAEELRARGLWRVPVIVITAAHDAARSAHEIGAADYLGK
                     PFELRALRSLVEKLLARATASAHV"
     gene            complement(527..1579)
                     /locus_tag="VFV99_09370"
     CDS             complement(527..1579)
                     /locus_tag="VFV99_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5059557.1"
                     /translation="MAAVLDAMPVGVYVCNGSGTLVFANKTAMRISGVSSLEEMREKA
                     AHPIAPPNLRTLDGKPIPQSFLPMQRALKGEEVVYEMEVFDSETRGTCVLRTRSSPVR
                     DRNGAIIGAVKVAIDVTKEHELARVKDEFIRQAAHELKTPIALIKATAETLASEEQPA
                     TTSLAALIRGVDRIDGLVSSLLDLADLEGGLFSFSRYPAQLERVITDAIGRFPINAAR
                     RVHVVSTPIVVQIDEARVRRAIYAVVHNAIKYSAPTSTVEVTLTREAKAARIAVRDHG
                     FGIPADKQPHVFEKYFRAHSGTPRDAGGIGVGLFVAREIIRQHGGEIWFESAENHGSV
                     FFIEMPIDEAKTPEAT"
     gene            1866..2396
                     /locus_tag="VFV99_09375"
     CDS             1866..2396
                     /locus_tag="VFV99_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX5059558.1"
                     /translation="MKSLVDRLSSYAEYHRDKRNIATHFVGIPMILVGTQSALARIGI
                     GPVNAAVGATGLATRYYRKLDPTYGAAMGAILGATCGMGTVLAAMPFPLWAGATAGLF
                     FGGWALQFVGHYFEGRKPAFLDDIMGLLDGPLFLVAETAFAVGLSPELRAEVELRAGP
                     TRWGKIADSNLAAVAS"
     gene            2459..2986
                     /locus_tag="VFV99_09380"
     CDS             2459..2986
                     /locus_tag="VFV99_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059559.1"
                     /translation="MLKLRLLVLLAIAGCGSSSPPATQPTAPPSPAPVADAAVDAAPD
                     ALPDALLNAPAWIFRYSTADRKETWTLRRDGGAALLIVESAQGERRYVGTARESDASI
                     ALDVSTGSAKMVLDCKREKLAVSATCNDKKAKPLDVLNCYHADFKTPMPFGPAPGIEY
                     VVDASCNGYRKVVAP"
     gene            3068..4417
                     /locus_tag="VFV99_09385"
     CDS             3068..4417
                     /locus_tag="VFV99_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059560.1"
                     /translation="MWTRCLTLIVVGGCGRIAFDPLGVGSGSDVPQDASGREFASVVA
                     GNGHTCVLSNRGEVACWGDNQYGKLGIGDAMNRGDAPDEVGLLLPRPDFGTVAKPTAL
                     TTTSDFSCALLSDDTAKCWGNNDGAQLGVGDYQSRGDMPGEMGDQLPTVQLAAGTHIT
                     ALVVGHYHGCALVAGSVRCWGSNDTSQNGYGDSIARDTVAELTTLPAVDLGPGFTVAK
                     LSAGFNWTCAISTAQTVKCWGYQPNGELGLGDTQTRGDAPSDMGAALPVVDVGAPVDL
                     IDAGYFHACALLADRVKCWGGGANGALGSGNMMDRGVAPGQMGAALPFVDLGTNARVT
                     ALSAGDRFTCAVLVGGDVKCWGYNNVGQLGLGDVSDRGDAPGEMGDALPRVPLPGPAR
                     AVAVGTRHACAILVSGGLVCWGSNANGQLGRGDTQNRGDQPNELDNVIMPSQLWPDP"
     gene            complement(4414..5841)
                     /locus_tag="VFV99_09390"
     CDS             complement(4414..5841)
                     /locus_tag="VFV99_09390"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HEX5059561.1"
                     /translation="MRIWALCLLTLIACGDSKPNVSPDASGTPDTTMGDADPGAVGDI
                     LDELNKIPGMTAVEGMTMTAGYRFFQMQYEQPVDHKNPGGAHFTQRMTLMYRNYAAPM
                     VVYNSGYFVSYTRGSRSQVTQLVDGNQLSMEYRYFEPSRPDPADWTKLDIEQAANDQH
                     RITQVLQARLFQGKWLTTGASKGGMTSLFHRRFFPDDVDGTVAFVAPIDYPEDAVQSP
                     TNRFFVFLENVGTDPTCRQKLKDFQNLALARRDAMKTRMRMAATYTAILGEDRALEFA
                     VEELPFIFWQYGSQSQCSKIPANTATDDAVFKFLDDTIDVTSYGDADLTDYLPYYHQS
                     ATQLGYPISDESYLVGLMYPGGDTAQAYVPSSIPAPVYDNGTAMHDFQDWLSASGEHI
                     LLIYGQNDPWSAGQATLGNATDSFKFIAPNGNHGSGIATLNADDEATATAAVMRWAGL
                     AMKKAKPLGLERTTEQDELALRRRR"
     gene            complement(5958..6206)
                     /locus_tag="VFV99_09395"
     CDS             complement(5958..6206)
                     /locus_tag="VFV99_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059562.1"
                     /translation="MLNGVDMMVTPYDPVTMRFWQLVTTGSVLHLNYSRDAVHWVELT
                     STDRGTDDLTAADLAFGAGRFGQTATGITTFDNLNLCP"
     gene            complement(6379..6729)
                     /locus_tag="VFV99_09400"
     CDS             complement(6379..6729)
                     /locus_tag="VFV99_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059563.1"
                     /translation="MVDIYADDEHVTIEVRGLHQFWAFKRKIHVPRSAVRSVRRLPSD
                     ALEGMWKGWRVPGTHLPGVIVAGTYFKDGERHFWDVRHADHAIEIELDGQRYQRLFVE
                     VADPDLAIRTLESH"
     gene            complement(6761..7513)
                     /locus_tag="VFV99_09405"
     CDS             complement(6761..7513)
                     /locus_tag="VFV99_09405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX5059564.1"
                     /translation="MKWILVLVLIGCVSPVKPSGGSASAKRAQREQLNELLPVALSTE
                     GPWRGPIVDAKIRIYADDEFRAQNLDWKRTFDDTLEYANAVLGAELGVRLVPDYREWE
                     HHAPGNSLDEDLKELEQLDDGGDALTVIGLTSSLSLVSATFEQLGYASVPGRHMMLRG
                     YADLVERKAFAESFPDLSRDEREKAHDARRRHKVTALLLHELGHNLGAPHDATEDTLM
                     SPTYSRNAAAFSAEAHATIQHTLDHRLGRSAD"
BASE COUNT         1254 a         2547 c         2475 g         1349 t
ORIGIN      
        1 atcaccagaa caccgacgag cgagacccac gggatgcgct ggcgaacctt cggcagaggc
       61 gatttcgatt gcgtcatgcc cccaagacag cggccgaaat cagcggtttc cgaggcgtcc
      121 ctgcgacgtc gcgccagtgt cgcaccggcc tacacgtgcg ccgatgcggt cgcgcgcgcg
      181 agtaacttct cgacgaggct gcgcagcgca cgcagctcga agggcttgcc gaggtagtcg
      241 gcagcaccga tctcatgggc gctgcgcgcc gcatcgtgcg ctgcggtgat cacgatgacc
      301 ggcacccgcc acagcccgcg cgcacgcagc tcctcggcga actgccagcc gttcatgccg
      361 ggcatgttca tgtcgagcag gatcaggtcc ggtaggcctt gcgattcgat cagcgtgagc
      421 gcggcgaggc cgtcgtcggc ttcgagcagc gtgcgaccgt ctgtgtgcag cccgtgcagc
      481 acgaccgcgc gcagatcctc gtcgtcctcg accaccagga tcctcgtcat gtcgcctccg
      541 gcgtcttcgc ttcgtcgatc ggcatctcga tgaagaacac gctgccatga ttctcggcgc
      601 tctcgaacca gatctcgcca ccgtgttggc gaatgatctc tcgcgcgacg aagagtccga
      661 caccgatgcc gccggcatca cgcggcgtcc cactgtgcgc gcgaaagtac ttctcgaaca
      721 cgtgcggttg cttgtccgca ggaatgccga acccgtggtc gcgaacggca atgcgtgccg
      781 ctttcgcctc gcgcgtgagc gtgacttcca ccgtcgacgt cggcgccgag tacttgatcg
      841 cgttgtgtac gacggcatag atcgcgcggc gcacacgggc ttcgtcgatc tggaccacga
      901 tcggcgtcga gacgacgtga acgcggcgtg cagcgttgat cggaaaccga ccgatcgcgt
      961 ccgtgatgac gcgctcgagc tgcgccgggt accgcgagaa gctgaacagc ccaccttcga
     1021 gatccgcgag atcgagcagc gagctgacga gcccgtcgat tcgatccaca ccgcggatca
     1081 acgcggcgag cgaggtcgtg gcaggctgct cctccgaagc gagcgtctcg gcggtcgcct
     1141 tgatcaacgc gatcggcgtc ttgagctcgt gcgccgcttg ccggatgaac tcgtccttca
     1201 cgcgcgcgag ctcgtgctcc ttggtgacgt cgatcgccac cttgaccgcc ccgatgatcg
     1261 cgccattgcg atcgcggacc ggggacgagc gcgtgcgtaa cacgcacgtg ccgcgcgtct
     1321 cgctatcgaa gacctccatc tcgtacacga cttcttcgcc cttcagcgcg cgctgcatcg
     1381 gcaggaagct ctgagggatg ggcttgccgt ccagcgtgcg caggtttgga ggcgcaatcg
     1441 gatgtgccgc cttctcccgc atctcttcca ggctcgaaac gccgctgatg cgcattgccg
     1501 tcttgttggc gaagacgagc gtgccgctgc cgttgcagac atagacgccg accggcatcg
     1561 cgtccagcac ggctgccatc tccagctcca tcaccagcct ctacattcag tggagcatgg
     1621 cgcacgcaac gtgaacatcg ggagccgcgc gccgagtctg cgtactgccg gttggtacgc
     1681 agtgcgggtt cgcgctcacg atctatcttt cgctcgcccg catggacacc gacgagcttc
     1741 gtgctcgcgc gagggcactg ggagtcccaa gcgcgccgta agcgtggcgg cttcaccacc
     1801 ccgtcccacc tgcggcgaca actcttctgt cggggaaatg attgcctgcg tagtaagctc
     1861 gacgcatgaa gtccctcgtc gaccgcctct cgtcgtatgc cgagtaccac cgcgacaagc
     1921 gcaacatcgc cacgcacttc gtcggcattc cgatgatcct cgtcggcacg cagtccgcgc
     1981 tcgcgcgcat cggcatcggt ccggtcaacg cggccgtcgg tgcgacgggg ctcgcgactc
     2041 ggtactaccg caagctcgat ccgacgtacg gcgcggccat gggcgcgatt ctcggagcga
     2101 cgtgcggcat gggcaccgtg ctcgccgcga tgccgttccc gctatgggcg ggtgccacgg
     2161 ccggcctgtt cttcggcggg tgggcgttgc agttcgtcgg tcactacttc gagggccgca
     2221 agccggcgtt cctcgacgac atcatgggcc tcctcgacgg tccgctgttc ctcgtcgccg
     2281 agaccgcgtt tgcggtcggt ctgtcgcccg agctccgcgc cgaggtcgag ctccgcgccg
     2341 gcccgacccg ctggggcaag atcgcagaca gcaatctcgc cgccgtcgcc agctgaaaac
     2401 gtcgcatcgc cgcctgccaa ccgctcccaa cgcggctggg actggtagcg tcggcgcgat
     2461 gttgaagctt cgcctcctcg tgctgctcgc gatcgccggt tgcggctcgt cgtcgccgcc
     2521 tgctacgcag ccgaccgcgc ctccctcacc agcgcccgtc gccgatgcag ctgtcgacgc
     2581 cgcccccgat gcgctgcccg atgcgctcct caatgcgccc gcgtggatct ttcgctacag
     2641 caccgccgac cgcaaagaga cgtggacgct gcgccgcgac ggcggtgctg cactcctgat
     2701 cgtcgagagc gcgcaaggcg agcgtcgcta cgtcggcacc gcacgcgaga gcgacgcgtc
     2761 gatcgccctc gatgtctcga ccggcagcgc gaagatggtg ctcgactgca agcgcgagaa
     2821 gctcgcggtc agcgcaacgt gcaatgacaa gaaggcgaag ccgctcgacg tgttgaactg
     2881 ctatcacgcg gacttcaaga cgccgatgcc gttcggaccg gcgcccggca tcgagtacgt
     2941 cgtcgacgca agctgtaatg gctaccgcaa ggtcgtcgcg ccgtagcgtg cgcaatccgt
     3001 gcattttcgt cgagccggcg gaaacatgcg cgtcacacgc cgtgcgtgag agactggggt
     3061 gtcggcgatg tggacacggt gcctcacgct catcgtggtt ggtggttgcg ggcgcatcgc
     3121 cttcgatccg cttggggttg gcagcggcag cgacgtgccg caggacgcga gcggccgaga
     3181 attcgcgagc gtcgtcgccg ggaacggtca cacgtgtgtg ctctcgaatc gcggcgaggt
     3241 cgcctgctgg ggcgacaacc agtacggcaa gctcggcatc ggcgacgcga tgaaccgcgg
     3301 cgacgcgccc gatgaagtcg gactgttgct accacgtcct gacttcggca cagtcgccaa
     3361 gccaaccgcg cttacgacga cgagcgactt ctcgtgcgcg ctgttgtccg acgacactgc
     3421 gaagtgctgg ggcaacaacg acggcgcgca gctcggtgtc ggcgactatc aatcgcgcgg
     3481 cgacatgcca ggcgagatgg gcgatcagct gccgacggta cagctcgcag ccggcacgca
     3541 catcaccgcg cttgttgtcg gccactatca cggctgtgct ctcgtcgctg gttcggtgcg
     3601 ctgctggggc agcaacgaca cctcgcagaa tggctacggc gacagcatcg cgcgcgacac
     3661 ggttgccgag ctgaccacat tgcccgcggt ggacctcggt cccggattca ccgtcgcgaa
     3721 gctttcggcg gggttcaatt ggacctgcgc gatctcgacc gcgcagacgg tcaagtgctg
     3781 gggctatcag cccaacggcg agctcggcct cggtgacacg cagacccgcg gcgatgcgcc
     3841 gtccgacatg ggtgctgcgc taccggttgt cgatgtcggc gcaccggtcg atctgatcga
     3901 cgccggctac tttcacgcgt gcgcgctgct cgccgatcgc gtgaagtgct ggggcggcgg
     3961 cgccaacggc gcgctcggca gcggcaatat gatggatcgt ggcgtcgcgc caggacagat
     4021 gggcgcggcg ctgcccttcg tcgatctcgg taccaacgcg cgggtgaccg cgctgtccgc
     4081 cggcgaccga ttcacatgtg ccgtgctcgt cggtggcgat gtgaagtgct ggggctacaa
     4141 caacgtcgga cagctcggcc ttggtgatgt cagcgaccgc ggcgacgcac cgggcgagat
     4201 gggcgatgcg ttgccgcgcg ttccgttacc gggacccgcg cgtgcagtgg cggtgggcac
     4261 ccgccacgca tgcgcgatcc tcgtctcggg cggtttggtg tgctggggca gcaacgcgaa
     4321 cggccagctc ggccgcggcg atacccagaa ccgtggcgat cagcccaacg agctcgacaa
     4381 cgtgatcatg ccgtcgcagc tgtggccgga tccttagcgg cgccgacgca gcgcgagctc
     4441 gtcttgctcg gtggtgcgct cgagaccgag tggcttcgcc ttcttcatcg cgagccctgc
     4501 ccagcgcatc accgccgcag tggcagttgc ttcgtcgtcg gcgttcagcg tcgcgatgcc
     4561 cgagccgtgg ttgccgttcg gtgcaatgaa cttgaacgag tcggtcgcgt ttccgagcgt
     4621 ggcctggccc gcgctccacg gatcgttctg gccgtagatg agcaagatgt gctcgccgct
     4681 cgccgagagc caatcctgga agtcatgcat cgccgtgccg ttgtcgtaga ccggcgccgg
     4741 aatgctcgac gggacgtatg cctgcgcggt gtcaccaccc gggtacatca ggccgacgag
     4801 gtagctctcg tcgctgatcg gatagccgag ctgcgtcgcg ctctggtggt agtacggcag
     4861 gtagtccgtg agatcggcgt cgccgtacga cgtgacatcg atcgtgtcgt cgaggaactt
     4921 gaacaccgcg tcgtcggtcg ccgtgttcgc cggaatcttg ctgcactgcg actggcttcc
     4981 gtactgccag aagatgaacg gcagctcttc gaccgcaaac tcgagcgcac gatcctcgcc
     5041 gaggatcgcg gtgtacgtcg cggccatccg catgcgcgtt ttcatcgcat cacgccgcgc
     5101 gagtgcgagg ttctggaagt cttttagctt ctggcggcac gtcggatcgg tgccgacatt
     5161 ctcgaggaac acgaagaagc ggttcgtcgg cgactgcacg gcgtcttccg ggtaatcgat
     5221 cggcgcgacg aacgcgaccg tgccgtcgac atcatccggg aagaagcggc ggtgaaacag
     5281 cgacgtcatg ccacccttgc tcgcgccggt cgtcagccac ttgccttgga acaagcgagc
     5341 ctgcagcacc tgcgtgatgc ggtgctgatc gttggcggcc tgctcgatgt cgagctttgt
     5401 ccagtcggcc ggatcggggc gcgacggctc gaagtagcga tactccatcg acagctggtt
     5461 gccgtcgacg agctgcgtga cctgcgagcg gctgccgcgc gtatacgaga cgaagtaacc
     5521 gctgttgtag acgaccatcg gcgccgcgta gttgcggtac atcagcgtca tgcgctgcgt
     5581 gaagtgcgcg ccacccgggt tcttgtgatc gaccggctgc tcgtactgca tctggaagaa
     5641 gcgataaccg gccgtcatcg tcatgccctc gaccgcggtc atgccgggaa tcttgttgag
     5701 ctcgtcgaga atgtcgccga cggcacccgg gtccgcatcg cccatcgtcg tatcgggcgt
     5761 tccggacgca tcgggagaca cgttcggctt cgaatcaccg catgcgatca gcgtaagcag
     5821 acaaagcgcc cagatcctca tggccgcggg cgtagcacgg ggcccgcaac gcaccggccg
     5881 atcggcgcta accggcgctc gctcctagcg aggcagcgat gactcagggg tgcgaatcgc
     5941 gtgtttcagc gtcgcgctta cggacacagg ttgaggttgt cgaacgttgt gatcccggtc
     6001 gccgtctgcc cgaagcgccc ggcgccaaac gccagatccg ccgcggtgag atcgtcggtg
     6061 ccacggtcag tcgacgtcag ctcgacccag tgaaccgcgt cacgtgaata gttcagatgc
     6121 agtaccgagc cagtcgtcac cagctgccag aaccgcatgg tgaccgggtc atacggggtc
     6181 accatcatgt cgacgccgtt caacacgaac ctgaccggtc agcccgtcgc catcaatgac
     6241 ggagccgttg gcgtcaccga ccgtgtcgaa gtcgatgcgt ccacagctgc acagcaccac
     6301 gacgagcaag tgcctcacca gacccgtctc cagggtaaca ccaagcctgg cgatgctcct
     6361 gtccttctcg agtgcgagct agtgcgattc gagcgtgcga atggcgagat ccggatcggc
     6421 gacctcgacg aacaagcgct ggtaacgctg gccgtccagc tcgatctcga tcgcgtggtc
     6481 cgcgtgcctg acgtcccaga agtgacgctc gccatccttg aaataggttc ccgccacgat
     6541 cacgccgggc agatgcgtac ccggcacgcg ccagcccttc cacatgccct cgagtgcatc
     6601 gctcggaagc cggcgcaccg agcgaacggc gctgcgcggc acgtggatct tgcgcttgaa
     6661 cgcccagaac tggtgcaaac cgcgaacctc gatcgtcacg tgctcgtcgt cagcgtagat
     6721 atcgaccatg actgcaccgt accaccgcgc tcggtgtcgg ctagtccgcg gaccgaccga
     6781 gccgatgatc gagcgtgtgc tggatggtcg cgtgcgcctc ggcactgaac gccgccgcgt
     6841 tgcgtgaata cgtcgggctc atcagcgtgt cctcggttgc gtcgtgcggc gcgccgaggt
     6901 tgtgaccgag ctcgtggagc agcagcgccg tgaccttgtg ccggcgacgc gcatcgtgcg
     6961 ccttctcgcg ttcgtcgcgc gagagatccg gaaacgactc cgcaaatgct ttgcgctcga
     7021 cgagatctgc gtagccgcgc aacatcatgt gccgccccgg cacgctcgca tagccgagct
     7081 gctcgaacgt ggccgagacg aggctaagcg atgacgtgag tccgatgacc gtgagcgcat
     7141 cgccgccatc gtcgagctgc tcgagctcct tcagatcctc gtcgagcgag ttgcccggcg
     7201 catggtgctc ccactcgcgg tagtccggca cgagacgcac gccgagctcc gccccgagca
     7261 ccgcgttcgc atattcgagc gtgtcgtcga acgtccgctt ccagtcgagg ttctgcgccc
     7321 ggaactcgtc atcggcgtag atgcggatct tcgcgtcgac gatcgggccg cgccacggcc
     7381 cttcggtcga cagcgcgact ggcaggagct cgttcaattg ctcgcgctgc gctcgcttcg
     7441 cggaggcgct gccacctgat ggcttgaccg gtgacacgca gccgatcagc acgagcacga
     7501 ggatccactt cacagcgcca cgcattgtac ggccgctatt tgccgagcac gcgctttcgt
     7561 gcggcctgca cgacgagcgg cggcgcaccc gtacctttcg cgatctggcg caaggcatcg
     7621 ggcgg
//
LOCUS       DASUFX010000241        11568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1565905, whole genome shotgun sequence.
ACCESSION   DASUFX010000241 DASUFX010000000
VERSION     DASUFX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565905"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFV99_09410"
     CDS             complement(<1..284)
                     /locus_tag="VFV99_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5059565.1"
                     /translation="MNLQSMLEKCRRDQWRVGDLDWTGTPRAMSRDDEIAIVQCLTDM
                     AGIERLAGELFETQRRRTDDPVLREIFATFVTDEIRHSHAAQMLADYYNV"
     gene            complement(281..1453)
                     /locus_tag="VFV99_09415"
     CDS             complement(281..1453)
                     /locus_tag="VFV99_09415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5059566.1"
                     /translation="MELPPLHVRPRFSDLMYVSPYTESALDRWRAAGDPLADDVVTAL
                     AARGPLGKIHDLLSVVRARAAEGDAQCQHLLREATAVPPWADFAAMAKGQRMIASYAP
                     FMAASLFSGSLVGGAMFYKAALVTSMTGMFSGDPLQRLTETTALVTRMAMPGSLEPGQ
                     GAHEVMLRVRLLHAGLRRVLVDSGRFRHPEEVPINQQDLAITLALFCYVNIRNLLQMG
                     IRLDREQIDSFVLLWRYAGHVLGIDHALLPTTIEDQQAFYLSSCKHQVRPEKVMPQTK
                     TLLDAVAKQAKLVPYPIAQTFLHQVTCYLSGNDYISGMQLDDRGDRYFGVRGLRYLGQ
                     AASFVHRHMPFGERVFYRAGLQMYRRELAKNERKKKYRYRVQMRENPAHRAEEKRA"
     gene            1456..2151
                     /locus_tag="VFV99_09420"
     CDS             1456..2151
                     /locus_tag="VFV99_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5059567.1"
                     /translation="MKYETEQMLGSTRIPFSPLKVPSQRRAWVTYNSVLDATARLLET
                     TGFAALTTNHVADLAGVSIGSLYEYFPDKETLVAELARRTLREIMGEVTGGLRDAVEL
                     GGEPGLRRGVGILFEVVERRRALVRELWQLPYLATLDEFKQLPKVTVEITRQLSPAFA
                     KKRVLRNLEASSWLLTVMVGNAVVHGAAARPRTLARKDVEDTLVQMLALLLLPARPRK
                     GAARRLVSSQDKI"
     gene            complement(2067..2789)
                     /locus_tag="VFV99_09425"
     CDS             complement(2067..2789)
                     /locus_tag="VFV99_09425"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5059568.1"
                     /translation="MRRAVVVSMLVGVAVRANATQQKAPCAGCTFEISSAATEPVPLV
                     VVMHGDTDTARQRAMKWRAAVERKGWALLALDCPDELGCDRSWYKWDHDPAWVHEQVR
                     EVIAHHPIDRTRIYLVGWSGGATYIGMHLQAWPPTFAAAVIHGGGVEPLTSECPDRPF
                     PAYFLVGDENPSHGGEKRLRAFFTACNEDVRWDLVRGADHAGEDAALTEAKADQILSW
                     LETKRRAAPLRGRAGNSSNASI"
     gene            2910..4826
                     /locus_tag="VFV99_09430"
     CDS             2910..4826
                     /locus_tag="VFV99_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX5059569.1"
                     /translation="MKWSVSVAGIWALSCASSPQRTSGPRTVTVEKPKTRASSQQVTA
                     PILTEGECITAELFEAGVAKGAICVADAQARGLTIVDLTDTWTPTIFQPTREGQTPTF
                     RQRYLDLAQEKIEKTETGIEALAELYGIVPAFAIVKARLADDSRHTCNAAIDVSKILL
                     LDRTYSQDNAKEVKASIFGHAWLGQQLEKERAKRKLADVAALEADPKWHERYIRWMKL
                     DELHLALLTAQQRLHCEGWLSDKEVTGEFGWRMGDAVEHFQRRNFLMPTERLDKETRE
                     AMQLDSRELDFRLALRILRERVVEAAGILEDGTAGTGPQPVLGRMLDPEAMRAARGHK
                     PLPNAAPDLVGAATEAAAKALGWTGPADVRAFFNKFPTGTRVAIALPAAPSYYKPHME
                     LSVEIDRGDVYYDDRPTYHYIQHRPTLVMYVDDAGTKRPLVRWPTTIGGWADQRMKGG
                     WLAQKWKESEVGPRIWRDLTVGPTWMPPKTTPDKDLVRNLYNGHWALKDDLLGPGPRS
                     AYGMVLLEHLQIVKQKDGTERLDNNGIGTHGSASVTSIVSGTSHGCHRLYNQLAVRLA
                     DYLLHHRDHAVKGEQKVGYLRRVWHNNESFKAEIESRGFQYELTPPIDVTVTKGNILS
                     KRKTPPRNSAPALP"
     gene            4897..6801
                     /locus_tag="VFV99_09435"
     CDS             4897..6801
                     /locus_tag="VFV99_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059570.1"
                     /translation="MAAPKNTIDIEDLPAPVLSVLRKSITERWYPLGKQTSNAYIWLY
                     LLGIVALGWGLFDGFGVPRSAAQDSGSLVFYIGGVGLIAWGVISQRKRKALRALLGFT
                     PGVYVLGSRLVDARTRTLGVHVLLDLKPDIVHRYVNGGYRNTTITWHGHTFVFGRKDA
                     ATSALDAIGERFGELQKAFEANEYDQLLVLDPLVIGLAMAETEKEAAFKRGDKIREKP
                     KASWTGPLVALAVTGVVAPGAWYLRNYLSLESAYDNIHSTYDVDEWVEHGGDEARGHK
                     KRMEIELTDVLRYNSENANNLRDVLAKYPDAPAALKKPVEDALHARYEKARKAALALS
                     ASEQLTWYINTVYDRLDKGGVASMQVKVARTDNTELAKLDEVVAANPKLSKTIVPVAK
                     YFGTSDDESRTTRLKTAIQTGLGEFFPSDVMTFADAAQDAKDAAVIEIFYVISPKYSR
                     DGVPSLYSEVDKFNKPIPGAMSYPGIMFELGATLQVPGGPPAQKVMFQATPSPHINVQ
                     GYGDEGLDNGAVYQAMAESAFADLERKLVGALGGKAPPESADDSEGKADDASLDNACP
                     GLEDAIQTFTACTKITDTERERVLDALKDSSSSGDASAACRKVIALLQKSIKANHCTA
                     APAPDDTPAP"
     gene            complement(6812..7723)
                     /locus_tag="VFV99_09440"
     CDS             complement(6812..7723)
                     /locus_tag="VFV99_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5059571.1"
                     /translation="MSFVVAALIALMVVAALVGVVLIRLRRAAARIEPTDFSGITPIP
                     GAGGRGPPLPVVLVHGLFGFDRIGVPGVKVHYFRGIVKHLEKLGCHAHAVRLPASASV
                     PDRAKILADRITALPHERVDIIAHSLGGLDARYALAKLGLASKVRALVTIGTPHRGTP
                     LADLATDGALGLARKAIGALGLPLHALDWLSTSALERFNAEIKDVPGVRYTCVVGGIK
                     NPQTPIALALSPIHAYLRRLAGPNDGLVPISSQFWGETLAEIEADHWQQVGWRMSMRG
                     TFDALGMYAYIVSRLGDAPPKRQPSAA"
     gene            7748..8056
                     /locus_tag="VFV99_09445"
     CDS             7748..8056
                     /locus_tag="VFV99_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059572.1"
                     /translation="MRRLVWLLTVIACGPRAVPAGTPEQSVHDAMVLVCDAPTRAKGE
                     GASRSDAIAAHLTDGVGNVKVLTTVEGWKTDGIDQRELARLLKQGRINRCALRDTANE
                     "
     gene            8071..8460
                     /locus_tag="VFV99_09450"
     CDS             8071..8460
                     /locus_tag="VFV99_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059573.1"
                     /translation="MKRVAILLFAIAACGGDKAKTQSDTPSHDTSKPQTLAEAVTYLC
                     AAPARAKADPAYKQGCLEKDQACSGDVIEQVKLLGKHMEENQSNERVLAFLKVEGQQK
                     GAELAKLTQEAGVTQCELADIYQAPAQ"
     gene            complement(8454..8642)
                     /locus_tag="VFV99_09455"
     CDS             complement(8454..8642)
                     /locus_tag="VFV99_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059574.1"
                     /translation="MAAVFALVFLFVLASAGLLVKTMMSDASFASIFALFTFLALGSG
                     VFIGLFKLSRKWEDEVPH"
     gene            8963..9625
                     /locus_tag="VFV99_09460"
     CDS             8963..9625
                     /locus_tag="VFV99_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5059575.1"
                     /translation="MSRFIFPEWTETLKKWVNLLVLGAPVYLVALIAYGVTPEALRIG
                     YQPDQPVPYSHALHAGELGIDCRYCHSVVERGPKAAIPPAAVCMNCHSTVRKDSDVLL
                     PIRQAFAENEPVHWTRVTDLPDYVYFNHQAHVNAAVGCESCHGRIDQMVKVYQAKPLT
                     MEWCLACHKNPTPNLRKPENVTKMGYDDPKSMPHRTPNEGQDVKRQNDVNPPINCSTC
                     HR"
     gene            9640..>11568
                     /locus_tag="VFV99_09465"
     CDS             9640..>11568
                     /locus_tag="VFV99_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX5059576.1"
                     /translation="MSAKEHDHDHHHAHEHDHAHPHTHDDQPQAPLPATPTHGYWKSI
                     RELEGKADWQLEPSTREFAPGADEPPPIDPMSRRNFFHLMGASMGMAGLAVGAGCRRY
                     EKEEIVPLARRPENQTPGTTLEFATTYDLAGAAHALVATSFEGRPIHIDGNPDHPFAS
                     GAIVPGTKKHAGAHTFAQASILNLYDPDRSTTPLNKGNGASMESFQVALGELKKAIAA
                     GGARVLSEASSSPTLQALKNRLVQGGVQWHEYEPLSWDNERAGTKAAFGRPVRPLAKL
                     DKCETIVTIDCDIFVEHPASMRYSRDFALSRRKDGSLGPGKMNRLWAAESVMSNTGAM
                     ADHRLGMRSELGLPFAMALDASLSGTGLPNSEFLKESKVAQFISVLTEELKANTGRAV
                     VIAGRRQPAEVHALVAKINATIGAPGATLDYLEDPEPGRPSHLQSITALVQDMAAGRV
                     PTLIILGGNPVYDAPADLDFATALSKVTSSVHLSEYADETSQKVSWHVPKAHFLEAWG
                     DARTWDGTVSIAQPLIAPIFGAWSSIELISRLLGDEVSGEALVKGTLGTLGATNWRQH
                     VHDGFIANTALPPAQVSVAAMPPFNLTPSQQAGSKRKNGELEVVYHFSSHTYDGRFSN
                     NPWLWETPDFLTKICWDNY"
BASE COUNT         2139 a         3782 c         3831 g         1816 t
ORIGIN      
        1 tggacgttgt agtagtcggc cagcatctgc gcggcgtgcg agtggcggat ctcgtcggtg
       61 acgaacgtcg cgaagatctc gcggagcacc ggatcatcgg tgcgccggcg ttgggtctcg
      121 aacagctcgc cggcgagccg ctcgatgccg gccatgtcgg tgaggcactg cacgatcgcg
      181 atctcgtcgt cgcgtgacat cgcgcgcggt gtccccgtcc aatcgagatc gccgacgcgc
      241 cactggtcgc gccggcactt ttcgagcatc gattgcagat tcatgcgcgt ttctcctctg
      301 cgcggtgcgc ggggttctcg cgcatctgga cgcggtagcg gtacttcttc ttgcgttcgt
      361 tcttggccag ctcgcggcgg tacatctgca ggcccgcgcg gtagaacacg cgctcgccga
      421 acggcatgtg gcgatggacg aacgacgctg cctgaccgag gtagcgcagg ccgcggacgc
      481 cgaagtagcg gtcgccgcga tcgtcgagct gcatgccgga gatgtagtcg ttgcccgaga
      541 ggtagcaggt gacttgatgc aagaacgtct gggcgatcgg atacggcacg agcttggctt
      601 gcttcgcgac cgcgtcgagc agtgtcttcg tctgcggcat cactttctcg gggcgcacct
      661 ggtgcttgca gctcgacaga tagaacgcct gctggtcttc gatcgtcgtt ggcaggagtg
      721 cgtggtcgat accgagcacg tggccggcgt agcgccagag cagcacgaag ctgtcgatct
      781 gttcgcgatc gagccggatg cccatctgca acaggttgcg gatgttgacg tagcagaaca
      841 gcgcgagcgt gatcgcgagg tcctgctgat tgatcggtac ctcttccggg tgccggaacc
      901 ggccgctgtc gacgagaacg cggcgcagtc ccgcgtgcaa caggcgcacg cgcagcatca
      961 cctcgtgggc gccctggccg ggctcgagcg agcccggcat cgccatgcgc gtcacgagcg
     1021 cggtggtttc ggtgaggcgc tgcagcggat caccggagaa catgccggtc atcgaggtga
     1081 cgagcgcggc cttgtagaac atcgcgccgc cgacgagcga tccggagaac aacgacgccg
     1141 ccatgaacgg cgcgtagctc gcgatcatgc gctggccttt tgccatcgcc gcgaaatccg
     1201 cccacggcgg caccgccgtt gcctcgcgca acaggtgctg gcactgcgca tcaccttcgg
     1261 ccgctcgcgc gcgcacgaca ctcaacagat cgtggatctt gccgagcggg ccccgagcgg
     1321 cgagcgcagt cacgacgtca tcggcgagcg gatcgccggc cgcgcgccag cggtcgagcg
     1381 cggactcggt atacggcgac acgtacatga ggtcgctgaa ccgcggcctg acgtgcagtg
     1441 gcggaagctc catacatgaa gtatgaaacc gagcagatgc tcgggtctac tcggattcca
     1501 ttttcgccgc tcaaggtgcc tagccagcga cgagcatggg tcacgtacaa cagcgtgctc
     1561 gacgcgactg ctcggcttct ggagaccact ggtttcgcgg cgttgacgac aaatcacgtc
     1621 gccgatctcg caggcgtcag catcggatcg ctctacgaat actttcccga caaagaaacg
     1681 ctcgtcgccg agctcgcgcg ccgcacgctg cgcgagatca tgggagaggt taccggcggg
     1741 ctgcgcgatg cggtggagct aggtggcgag cccggcctgc gccgtggcgt cggtattctg
     1801 ttcgaggtcg tcgagcgccg gcgcgcactg gtgcgcgagc tgtggcagct gccgtacctc
     1861 gcgacactcg acgagttcaa gcaactgccg aaggtgaccg tcgaaatcac gcgccagctg
     1921 tcgcccgcgt tcgcgaagaa gcgcgtgctg cgcaacctcg aagcgtcgag ctggctgctc
     1981 accgtgatgg tcggtaatgc agtcgtgcat ggcgcagcgg cgcggccgcg gacgctcgcg
     2041 cgcaaggacg tcgaggacac gctcgttcag atgctcgcgt tgctgctatt gccggcacgg
     2101 ccgcgcaagg gtgcggctcg ccgcttggtc tcgagccaag acaagatctg atcggctttc
     2161 gcctcggtga gcgccgcgtc ctcgccggcg tgatcggcac cgcgcacgag atcccagcgc
     2221 acgtcttcgt tgcacgcggt gaagaatgcc cgcaggcgct tctcgccgcc gtgcgacgga
     2281 ttctcgtcgc cgacgagaaa gtacgccggg aatggccgat ccgggcattc gctggtgagc
     2341 ggctcgacgc caccaccgtg gatcacggca gcggcgaacg tcggcggcca cgcttgcagg
     2401 tgcatgccga tatacgtggc gccacccgac cagccgacga gatagatccg cgttcgatcg
     2461 atcggatggt gcgcgatcac ttcacgcacc tgctcgtgaa cccacgcggg atcgtgatcc
     2521 cacttgtacc agctgcgatc gcagccaagc tcgtcggggc aatcgagcgc gagcagtgcc
     2581 caacctttcc gctcgaccgc cgctcgccac ttcatcgcac gctggcgtgc agtgtcggtg
     2641 tcgccgtgca tcacgacgac gagtggcacg ggctccgtcg cagcggacga gatctcgaac
     2701 gtgcatccag cacacggcgc cttctgctgc gtggcgttag cgcgcaccgc aacgccaacc
     2761 agcatcgaga ccacgactgc gcggcgcatg gatcgagacg ttgcacgagg tgcgccgctg
     2821 ctcacatgtg cgacgacctc tgataagcac gctgcgaatt cgtcacgtgc cgtttccgtc
     2881 gaagagcgac acagttgagg aacgtgggca tgaaatggtc ggtatcggta gcgggcattt
     2941 gggcgctcag ttgtgcaagc tcaccgcagc gcacgtccgg accccggaca gtaacggtcg
     3001 agaagccgaa gacgcgcgcg agctcgcagc aggtcacggc gccgatcctg accgagggtg
     3061 agtgcatcac cgccgagctc ttcgaggccg gggtcgcgaa gggcgcgatc tgcgtcgccg
     3121 atgcgcaggc acgcgggctc acgatcgtcg acctcaccga cacgtggacg cctacgatct
     3181 tccagccgac gcgcgagggc cagacgccga cgttccgcca gcgctatctc gatctggcgc
     3241 aggagaagat cgagaagacc gagacaggca tcgaagcgct cgccgagctg tacggcatcg
     3301 tgcccgcgtt cgcgatcgtg aaggcgcggc tcgccgatga ctcgcgtcac acgtgcaatg
     3361 ccgcgatcga cgtgtcgaag atcctgctgc tcgatcgcac ctactcgcag gacaacgcga
     3421 aggaagtaaa agcctcgatc ttcggccacg cgtggctcgg ccagcagctc gaaaaggaac
     3481 gcgccaagcg caagctcgcc gacgtcgcgg cgctcgaagc cgatcccaaa tggcacgagc
     3541 gctacatccg gtggatgaag ctcgacgagc tccacctcgc gctgctcacc gcgcagcagc
     3601 ggctgcactg cgaaggctgg ctgtccgaca aggaagtcac cggcgagttc ggctggcgca
     3661 tgggcgatgc agtcgagcac ttccagcgtc gcaacttcct gatgccgacc gagcggctcg
     3721 acaaggagac gcgcgaagcg atgcagctcg atagccgcga gctcgacttc cggcttgcat
     3781 tacgcatcct gcgcgagcgc gtcgtcgaag ccgccggcat cctcgaggac ggcaccgccg
     3841 gcaccggccc acagcccgtg ctcggccgca tgctcgatcc ggaagcgatg cgcgcggcgc
     3901 gtggtcacaa gccgttgccg aacgcggcgc cggatctcgt cggtgctgcc accgaagccg
     3961 ctgccaaggc gctcggctgg accgggcccg ccgacgtccg cgcgttcttc aacaagttcc
     4021 caaccggaac gcgtgtcgcg atcgcgctgc ccgccgcgcc gtcctactac aagccgcata
     4081 tggagctgtc ggtcgagatc gatcgcggcg acgtctatta cgacgatcgc ccgacgtacc
     4141 actacatcca gcaccgcccg acgctcgtga tgtacgtcga cgacgcgggc acgaagcgcc
     4201 cactcgtgcg ctggccgacg acgatcggcg ggtgggccga tcaacgcatg aagggcggct
     4261 ggctcgcgca gaagtggaaa gagtccgagg tcggcccgcg catctggcgc gacctcacgg
     4321 tcggtccgac gtggatgccg ccgaagacga caccggacaa agatctcgtt cgcaatctgt
     4381 ataacggtca ctgggcactc aaggacgatc tgctcggtcc cggcccgcgc tcggcctacg
     4441 gcatggtgtt gctcgagcac ctgcagatcg tgaagcagaa ggacggcacc gagcgcctcg
     4501 acaacaacgg tatcggcaca cacggctcgg cgagtgtgac gtcgatcgtg agcggcacga
     4561 gccacggctg tcatcgcctg tacaaccagc tcgcggtgcg cctcgccgac tacttgctcc
     4621 accatcgcga tcacgccgtg aagggtgagc agaaggtcgg ctacctgcga cgcgtctggc
     4681 acaacaacga atcattcaag gccgagatcg agtcgcgcgg ttttcagtac gagctgacgc
     4741 cgccgatcga cgtcaccgtc acgaagggca acatcctctc gaagaggaag acgccgccac
     4801 gcaacagcgc accggcgctc ccgtagtcag accggcttcg tcgaccgtct ccgacacggc
     4861 tcgtgatacc gtcggtggtt cacgaggggg agtgcgatgg ctgcgcccaa gaacacgatc
     4921 gatatcgagg atcttcctgc gccggtgctg tcggtgctgc gcaagtccat caccgaacgc
     4981 tggtatccgc tcgggaagca gacgtcgaac gcgtacatct ggctctacct gctcggcatc
     5041 gtcgcgctcg gctggggcct gttcgatggc ttcggcgtgc cacgcagcgc agcgcaggac
     5101 tcgggctcgc tcgtgttcta catcggtggc gttggcctca tcgcgtgggg cgtcatctcg
     5161 caacgcaagc gcaaggcgct gcgtgcgcta ctcggattca cgcccggtgt gtacgtgctc
     5221 ggctcgcgac tcgtcgacgc gcgcacgcgc acgctcggcg tccacgtgct gctcgatctc
     5281 aagccggaca tcgtccatcg ctacgtcaac ggtggctatc gcaacacgac gatcacctgg
     5341 cacgggcaca cgttcgtgtt cgggcgcaaa gacgcggcaa cttcagcgct cgacgcgatc
     5401 ggcgagcgat tcggcgagct gcagaaggcg ttcgaggcga acgagtacga tcaactgctc
     5461 gtgctcgatc cgctggtgat cgggctcgcg atggccgaga ccgagaagga ggcggcattc
     5521 aagcgtggcg acaagatccg cgagaaacca aaggcgagct ggacggggcc gctcgtcgcg
     5581 ctcgcggtca ccggcgtcgt cgcgccgggc gcgtggtacc tgcgcaacta tctcagcctc
     5641 gagtccgcgt acgacaacat ccacagcacg tacgacgtcg acgagtgggt cgagcacggc
     5701 ggcgacgagg cgcgtggtca caagaagcgc atggagatcg agctcaccga cgtgttgcgc
     5761 tacaacagcg agaacgcgaa caacctgcgg gacgtgctcg cgaagtatcc cgatgcgccc
     5821 gcggcgttga agaagcccgt cgaagacgcg ctgcatgcgc gctacgagaa ggcacgcaag
     5881 gcggcgctcg cgctgtcggc ctccgagcag ctgacctggt acatcaacac cgtctacgac
     5941 cggctcgaca agggcggcgt cgcctcgatg caggtgaagg tcgcgcgcac ggacaacacc
     6001 gagctcgcga agctcgacga ggtcgtggcc gccaacccga agctgagcaa gaccatcgtg
     6061 ccggtcgcga agtacttcgg cacctccgac gacgagtcgc gcacgacgcg gctcaagacc
     6121 gcgatccaga ccggcctcgg cgagttcttc ccgtcggacg tcatgacgtt tgcggacgcc
     6181 gcgcaggacg cgaaggacgc agcggtgatc gagatcttct acgtgatcag cccgaagtac
     6241 tcgcgcgacg gcgtgccctc gctctattcc gaggtcgaca agttcaacaa gcccattccc
     6301 ggcgcgatgt cctaccccgg catcatgttc gagttgggcg ccacgctcca ggtgccgggt
     6361 ggcccgcctg cacagaaggt gatgttccag gcgacgccgt cgccgcacat caacgttcag
     6421 gggtacggcg acgagggctt ggacaatggc gccgtctacc aggcgatggc cgagagcgcg
     6481 ttcgcggatc tcgagcgcaa gctcgtcggc gcgctcggtg gcaaggcacc gccggagtca
     6541 gccgacgaca gcgagggcaa ggccgacgac gcgagcctcg acaacgcctg tcccggcctc
     6601 gaggacgcga tccagacgtt caccgcgtgc acgaagatca cagatacgga gcgcgagcgc
     6661 gtgctcgatg cgctgaagga cagctcgtcg tcgggcgatg ccagcgcggc gtgccgcaag
     6721 gtgatcgcgc tgctccagaa gtcgatcaag gcgaaccact gcaccgcagc gcccgcgcct
     6781 gacgacacgc cggcgccgta gcgcagtagc gtcacgcggc ggagggctgc ctcttcggtg
     6841 gcgcgtcgcc aagtcgcgac acgatgtacg cgtacatgcc gagcgcgtcg aacgtgccac
     6901 gcatcgacat gcgccagccg acctgttgcc agtgatcggc ttcgatctcg gcgagcgtct
     6961 caccccagaa ctgcgacgag atcggaacga ggccgtcgtt gggcccggcg agcctcctca
     7021 gatacgcgtg gatcggtgac agcgcgagcg cgatcggcgt ttgcggattc ttgatgccgc
     7081 cgacgacaca cgtgtagcgc acgcccggca cgtccttgat ctctgcattg aaccgctcga
     7141 gcgcggacgt cgacagccag tcgagcgcgt gcagcggaag gccgagtgcg ccgatcgctt
     7201 tgcgtgcgag cccgagcgcg ccgtcggttg cgagatcggc gagcggggtc ccgcgatgcg
     7261 gtgtgccgat ggtgacgagt gcgcggactt tgctcgcgag accgagcttc gcaagtgcat
     7321 agcgggcgtc gaggccgccg aggctgtgcg cgatgatgtc gacgcgctcg tgcggcagcg
     7381 cggtgatgcg gtccgcgagg atcttcgcgc gatccggcac cgacgcggag gcgggcaggc
     7441 ggaccgcgtg ggcgtgacag ccgagcttct cgaggtgctt cacgatgccg cggaagtagt
     7501 ggaccttcac gcccggcacg ccgatgcggt cgaagccgaa cagaccgtgc acgagcacca
     7561 ccgggagcgg aggaccgcgg ccgcccgctc ctggaatcgg cgtgatgccg gagaagtcgg
     7621 ttggctcgat gcgtgctgca gcgcgccgca gccggatcaa gacgacgccg acgagcgccg
     7681 ccacgaccat cagcgcgatc agcgcggcga cgacgaagct cacgcatgcg agtataggag
     7741 gtgcgggatg cggcggctcg tgtggttgct aacagtgatc gcctgcggcc cgcgtgcggt
     7801 gcctgccgga acgccggagc aatccgttca cgatgcgatg gtgctcgttt gcgacgcgcc
     7861 aacccgcgca aaaggcgagg gcgcgagccg ctccgacgcg attgcggctc atctgacaga
     7921 tggtgtcggc aacgtaaagg tgctgacgac cgtcgagggt tggaagaccg atggtatcga
     7981 tcagcgcgag ctcgctcggc tgttgaaaca agggcgtatc aatcgttgcg ccttgcgcga
     8041 taccgcgaac gaatgatcta gtctcggcgc atgaagcgcg tcgcaatcct gttgttcgcc
     8101 atcgccgcgt gcggcggtga caaggccaag actcagtccg acactccgtc gcacgacacg
     8161 tcgaaaccgc agacgctggc cgaagcggtg acgtacctgt gcgcagcgcc tgcgcgcgcg
     8221 aaggcggatc ctgcgtacaa gcagggctgc ctcgagaagg atcaggcgtg cagcggcgac
     8281 gttatcgagc aggtcaagct actcggcaag cacatggaag agaaccagtc gaacgagcgc
     8341 gtgctcgcgt tcttgaaggt cgagggccag cagaagggtg ccgagctcgc gaagctcacc
     8401 caggaggccg gcgtcacgca gtgcgagctg gccgacatct accaagcgcc tgctcagtga
     8461 gggacttcgt cctcccattt gcgcgagagt ttgaacaggc cgatgaaaac gcccgagccc
     8521 agcgcgagga atgtgaacag cgcgaagatg ctcgcgaacg atgcgtcact catcatcgtc
     8581 ttgacgagta gaccggccga cgcgagcacg aacaggaaca cgagagcgaa taccgccgcc
     8641 atgcggaggg gtattgcgcg cggcgtgccg agctaatcgg cgtagggccg ggcgaaaccg
     8701 cgcatacgct gcgcaacgca gctggtgcac gaaggcggtg cgcagttttt caagatcatg
     8761 tgctgcggca gacggtcgca gctcattccg aagatccgtg cggtgaattg tcgcgttctg
     8821 ttcggaggca acatgccgca gcaaatccgt cgacgagacg tgcgatcaca cgcatcgcat
     8881 gcgtcgggac ggtctctaga tcgtgcccgt gcaaccttgc gaagtactta cgactcgtgg
     8941 tgaaagccgc tgcagggaaa ccatgtcgag attcatcttc ccggaatgga ccgagaccct
     9001 aaagaaatgg gtcaacttgt tggtcctcgg cgctcctgtc tacctcgtcg cattgatcgc
     9061 gtacggcgtg actccggagg cgttgaggat cggctaccag ccggatcagc cggtgcccta
     9121 ttcacacgcg ctccacgcgg gtgagctcgg catcgactgc cggtattgcc actcggtggt
     9181 ggagcgtggc cccaaggcgg ccatcccgcc cgccgcggtg tgcatgaact gccactcgac
     9241 ggtgaggaag gactcggacg tcctgctgcc aattcgccag gcgttcgccg agaacgagcc
     9301 ggtgcattgg acgcgcgtca ccgaccttcc cgactacgtc tacttcaacc accaggcgca
     9361 cgtgaacgcc gccgtcggtt gcgagtcgtg tcacggccgc atcgatcaga tggtcaaggt
     9421 ctaccaggcc aagccgctga cgatggagtg gtgcctcgcg tgccacaaga atccgacgcc
     9481 gaatctgcgc aagcccgaga acgtcacgaa gatgggctac gacgatccga agtcgatgcc
     9541 gcaccgcaca ccaaacgagg ggcaggacgt caaacgacag aacgacgtca acccgcccat
     9601 taactgctcg acctgtcacc gctgaggagc cgagcaacca tgtcagccaa agaacacgac
     9661 cacgatcacc atcacgcgca cgagcacgac cacgctcacc cgcacacgca tgacgatcag
     9721 ccgcaggctc cgctgccggc gacgccgacg cacggctact ggaagtcgat ccgcgagctc
     9781 gaaggcaagg cggactggca gctcgagccg agcacgcgcg aattcgcgcc cggcgccgac
     9841 gagccgccgc cgatcgaccc gatgtcgcgc cgcaacttct tccatctgat gggcgccagc
     9901 atgggcatgg ccggcctcgc ggtcggcgcg ggctgccgtc gctacgagaa ggaagagatc
     9961 gttccgctcg ctcgccgtcc cgagaaccag acgcccggca cgacgctcga gtttgcgacg
    10021 acgtacgacc tcgccggcgc agcacacgcg ctcgtcgcga cgtcgttcga gggtcgcccg
    10081 atccacatcg acggcaaccc cgatcatccg ttcgcgagcg gtgccatcgt ccccggtacg
    10141 aagaagcacg ccggcgcgca cacgttcgcg caggcgagca tcctcaacct ctacgatccg
    10201 gaccgctcga cgacgccgct caacaagggc aacggcgcgt cgatggaatc gttccaggtc
    10261 gcgctcggcg agctcaagaa ggcgattgcc gccggcggcg cccgcgtgct cagcgaagct
    10321 tcgtcgtcgc cgacgctgca ggcgctgaag aaccggctcg tgcaaggcgg cgtgcagtgg
    10381 cacgagtacg agccactgtc gtgggacaac gagcgcgctg gtacgaaggc cgcgtttggt
    10441 cgccccgtgc gcccgctcgc gaagctcgac aagtgcgaga cgatcgtcac gatcgattgc
    10501 gacatcttcg tcgagcaccc ggcctcgatg cgctacagcc gcgacttcgc gctgtcgcgt
    10561 cgcaaggacg gctcgctcgg cccgggcaag atgaaccgcc tgtgggccgc tgagagcgtg
    10621 atgtcgaaca ccggtgcgat ggcggatcac cgcctcggca tgcggtccga gctcggcctg
    10681 ccgttcgcga tggcgctcga tgcgtcgttg tcgggcaccg gcctgccgaa cagcgagttc
    10741 ctcaaggaga gcaaggtcgc gcagttcatc tcggtgctca ccgaggagct gaaggcgaac
    10801 accggccgcg cggtggtcat cgcgggtcgc cgtcagccgg ctgaagtcca cgcgctggtc
    10861 gccaagatca acgcgacgat cggcgcaccg ggcgcgacgc tcgattacct cgaggatccg
    10921 gaaccgggtc gtccgtcgca cctgcagtcg atcacggcgc tcgtccaaga tatggcggcc
    10981 ggccgcgtgc cgacgctgat catcctcggt ggcaaccccg tctacgacgc acccgccgat
    11041 ctcgacttcg cgaccgcgct cagcaaggtg acgagctccg tccacctcag cgagtacgcc
    11101 gacgagacgt cacagaaggt ctcgtggcac gtgccgaagg cacacttcct cgaggcgtgg
    11161 ggcgacgcgc gcacgtggga cggcaccgtc tcgatcgcgc agccgctgat cgcaccgatc
    11221 ttcggcgctt ggtcgtcgat cgagctgatc tcgcgtctgc tcggcgacga ggtctccggt
    11281 gaggcgctcg tcaaaggcac gctcggtacg ctcggcgcca cgaactggcg gcagcacgtg
    11341 cacgacggct tcatcgcgaa caccgcgttg ccgcccgcgc aggtgtcggt ggcagcgatg
    11401 ccgccgttca acttgacgcc gagccagcag gccggcagca agcgcaagaa cggcgagctc
    11461 gaggtcgtct accacttctc gagccacacc tacgacgggc ggttctcgaa taacccctgg
    11521 ctgtgggaga cgcctgactt cctcacgaag atctgctggg acaactac
//
LOCUS       DASUFX010000242         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_175456,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000242 DASUFX010000000
VERSION     DASUFX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175456"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFV99_09470"
     CDS             <1..935
                     /locus_tag="VFV99_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5059577.1"
                     /translation="IEPPVPGLLDERTRREAKARLAAAVAHASRANAAIERAQVARDA
                     ATREAKRAQTLFEREAITAAERDQAADTEQLALRDLAAAQTERASAVAEADAIRAQLG
                     EASPQDKQRIVTVTAPISGRVLRVVRDSGGPVATGAPLVELGDTQSLEVVVDVLSSDA
                     ARIHPGMPVAIEAWGGDKNLSGTVAEIEPSAFTRISALGVEEQRVKVIVGLSEPPPTL
                     GDGFRVEARIFTWQGTNVLTIPTSAVFRDHGRWAVYAVEGGKARLRRIELGHRGRLDV
                     EVVSGITAGEAVVLHPSDRIRDGVKLTRYAHETP"
     gene            963..2309
                     /locus_tag="VFV99_09475"
     CDS             963..2309
                     /locus_tag="VFV99_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059578.1"
                     /translation="MLRMLFVACTLLAFACSDSKPAKKKKEKPTDVALTAGPVPVTLA
                     KLDAKRLRPITSIKEFVIIDPKGALSVGAPKAGAPPWVGEPADRWHDRTGFLEYDLDS
                     FGTLSRDSSATALILADATTPARLTLTSGIQAYGYKPVALAAGAANTAVLYQFGGEPW
                     ADWDKRADLTLRTDQLDKLAGQKPHTVLLQFTDATTTGELLEALTAVAETGAEQVRLH
                     RMMGWGAIAPADIVWVRNAVADDDIITEGEPATVTIAEAKVDGKVKAESVGTAMSSAS
                     PRFRYCYEKSGAKKPGKLELSFDVAQDGSVANAAAKGINAAIESCVAGAIFDSHVPTD
                     GVEGPAHATVPIGFGHGEEKAAAAAAAAAKNKVSGWWCWIHQDGSVGMCAREQDQCEA
                     QLYGFNQTAESLHDVASTNECKQQKTAWETESSRPLPTQAMCKAARDKGETCRPLK"
     gene            2385..2777
                     /locus_tag="VFV99_09480"
     CDS             2385..2777
                     /locus_tag="VFV99_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059579.1"
                     /translation="MAEKAENGVNEEILTNQESVLANQARIMAYAERVEQLIANQKTM
                     LANQKAILANQKAIQANQKKLDAIAANQKKLDTILGNQKAVLGNQKTIAGNQKKLDAV
                     LANQKTIAGNQKKLDAVLANQKRILAKK"
     gene            2868..>3629
                     /locus_tag="VFV99_09485"
     CDS             2868..>3629
                     /locus_tag="VFV99_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059580.1"
                     /translation="MSIGELSRVRRDPSAEDVSLFNEIERQLMIRRWCIVAPALMIAA
                     FLSEAAAYLSPEFPKSTVMTASVITLFGSLWLYARRKPSRRGAMMIWIIAGWSSAAYV
                     ATACVDSGRFHSLHVLGIATIVALTPAALSFTLVESIVSLGGAVIVWVAVCLGWKADP
                     NGVDLGGLATSSMYLSFISGATVLSLAWARRLRLREFVARRQIEEMHRFAVEEVLLRH
                     LPPKYVADVLAGTRALDAPPERRVVTVIFADIVSFT"
BASE COUNT          678 a         1180 c         1222 g          549 t
ORIGIN      
        1 ggatcgagcc cccggtgccg gggttactcg acgagcgcac gcggcgagaa gcgaaggcgc
       61 ggcttgcggc cgcggttgct cacgcgagcc gcgcgaacgc ggcgatcgag cgcgcgcagg
      121 ttgctcgcga tgccgcaacg cgcgaggcga agcgcgcgca gactctgttc gaacgtgaag
      181 cgatcaccgc tgcagagcgc gaccaggccg cggacaccga gcagctcgcg ctccgcgacc
      241 tcgcggccgc acagaccgag cgcgcgagcg cggtggcgga agcagatgcg atccgagccc
      301 agctcggcga agcgagtccg caagacaaac aacgtatcgt cacggtgacc gcaccgatca
      361 gcggacgcgt gctgcgcgtc gtgcgcgaca gcggcggtcc cgtcgctacc ggcgcaccgc
      421 tcgtcgagct cggcgatacg cagtcgctcg aggtcgttgt cgacgtgttg tcgagcgatg
      481 cagcccgcat ccatcccggc atgccggtcg cgatcgaagc ctggggcggc gacaagaacc
      541 tcagcggcac cgtcgccgag atcgaaccct cggcgttcac gcgaatctcc gcgctcggtg
      601 tcgaagagca gcgggtcaag gtgatcgtcg gtctcagcga gccaccgccg acgctcggcg
      661 acggcttccg cgtcgaagcg cggatcttca cgtggcaggg tacgaatgtg ttgacgatcc
      721 cgaccagcgc agtgtttcgc gatcacggca ggtgggccgt ctacgccgtc gagggcggca
      781 aggcgcgcct acgccggatc gagctcggtc accgtggccg gctcgatgtc gaggtggtgt
      841 cgggcatcac cgcaggggaa gcggtcgtgc tgcatccgag cgatcggatc cgcgacggcg
      901 tgaagctcac ccgctacgcg cacgagacgc cgtgagctcc cggctcgtgc gaagctcgcc
      961 cgatgctacg gatgttgttt gtcgcgtgca ctcttctcgc gtttgcttgc tccgattcga
     1021 aaccagcgaa gaagaagaag gagaagccga cggacgtcgc gctcaccgcg gggccggtgc
     1081 cggtcacgct cgcaaagctc gacgccaagc gactgcgacc gatcacgagc atcaaggagt
     1141 tcgtgatcat cgatccgaag ggcgcgctca gcgtcggtgc cccgaaggcc ggtgcgccgc
     1201 cgtgggtcgg cgaacccgct gaccgctggc acgaccgtac gggcttcctc gagtacgacc
     1261 tcgactcgtt tggcacgctg tcacgcgact cgtctgcaac cgcgctgatc ctcgccgatg
     1321 cgacgacgcc agcgcggctc acgctcacga gcggtatcca ggcgtatggc tacaagcccg
     1381 tcgcgctcgc tgccggcgca gcgaataccg ctgtgcttta tcaattcggc ggtgaaccat
     1441 gggccgactg ggacaagcgc gccgacctca cgctacgtac cgatcagctc gacaaactcg
     1501 cgggccagaa accgcacacg gtactgctgc agtttaccga cgcgacgacg accggcgagc
     1561 tactcgaagc gctcaccgcg gtcgccgaga ccggcgccga gcaagtgcgc ctgcatcgca
     1621 tgatgggctg gggtgcgatc gcacccgccg acatcgtgtg ggtgcgaaac gccgtcgccg
     1681 acgatgacat catcaccgaa ggcgagccag ccacggtcac catcgcggaa gccaaggtcg
     1741 acggcaaggt caaagcggag tcggtcggca ccgcaatgtc gagcgcgtcg ccgcgcttcc
     1801 gctactgcta cgagaagagc ggtgcaaaga agcccggcaa gctcgagctg tcgttcgatg
     1861 tcgcgcaaga tggctcggtc gcgaacgctg cagcgaaagg aatcaacgcg gcgatcgaga
     1921 gctgcgtcgc aggtgcgatc ttcgacagcc acgtgccgac cgacggcgtc gagggcccag
     1981 cgcacgccac cgttccgatc ggtttcggcc acggcgagga aaaagctgcc gccgcagcag
     2041 cagcggccgc caagaacaag gtgagcggtt ggtggtgctg gatccatcaa gacggcagcg
     2101 tcgggatgtg cgcgcgcgag caggaccagt gcgaggcgca actttacggt ttcaaccaga
     2161 cggccgagag cctccacgac gtcgcgtcga cgaacgagtg caagcagcag aagacggcat
     2221 gggagaccga aagctcgcgg ccgctaccga cgcaggcgat gtgcaaggcg gcgcgcgaca
     2281 aaggcgagac ctgccgcccg ctcaaatgac gccgctgatt cgtgctcgga acgcttgagg
     2341 cggtgtcccc gcgcgcttct aatgcgggac ctaggaggat tccgatggcg gagaaggctg
     2401 agaacggcgt gaacgaggag attcttacca accaggagtc ggtgctcgcg aaccaggcgc
     2461 gcatcatggc gtacgccgag cgcgtcgagc agctgatcgc gaatcagaag accatgctgg
     2521 cgaaccagaa agcgattctg gcgaaccaga aggcgatcca ggcgaaccag aagaagctcg
     2581 acgcgatcgc cgcgaaccag aagaagctcg acacgattct cggcaaccag aaggccgtcc
     2641 tcgggaacca gaagacgatc gccggcaacc agaagaagct cgacgccgtc ctggcgaacc
     2701 agaagacgat cgccggcaac cagaaaaagc tcgacgccgt cctcgcaaac cagaagcgaa
     2761 tcctggcgaa gaagtaacga ttcgcggccg ccggggccgt cacgcagctt ggcccctcgt
     2821 cgaggaagat cgcgccgttt ggcgtggatc cccggtatga tggtcgggtg tcgatcgggg
     2881 agctgtctcg ggtacggcgg gatccgtctg cagaagacgt ctcgctgttc aacgagatcg
     2941 agcgccagct gatgatccgg cgctggtgca tcgtcgcacc cgcgctgatg atcgcggcgt
     3001 ttttgtccga agccgcggcc tacctctcgc cggaatttcc caagtcgacc gtgatgacgg
     3061 cgtcggtcat cacgctgttc ggctcgctgt ggctgtatgc gcgacgcaag ccgagccgcc
     3121 gtggcgccat gatgatctgg atcatcgcgg gctggtcgag cgcggcctac gtcgcaaccg
     3181 cgtgtgtcga cagcggccgg tttcactcgc tgcacgtgct cggcatcgca acgatcgtcg
     3241 cgctgacgcc ggctgcgctg tcgttcacgc tcgtcgaatc gatcgtgtca ctcggcggcg
     3301 ctgtcatcgt gtgggtcgcg gtttgcctcg gctggaaagc tgacccgaat ggcgtcgacc
     3361 tcggcggcct cgccacgtcg tcgatgtatc tctcgttcat ctcgggcgcg accgtactct
     3421 cgctcgcgtg ggcgcgacgg ttgcgcctgc gcgagttcgt cgcgcgccgc cagatcgaag
     3481 agatgcaccg cttcgcggtc gaagaagtgc tgctgcgcca cttgccgccg aagtacgtcg
     3541 ccgacgtgct cgccggcacg cgcgcactcg acgcgccacc cgagcgccgg gtcgtcaccg
     3601 tgatcttcgc cgacatcgtg tcgttcacg
//
LOCUS       DASUFX010000243        12595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1982371, whole genome shotgun sequence.
ACCESSION   DASUFX010000243 DASUFX010000000
VERSION     DASUFX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982371"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VFV99_09490"
     CDS             <1..1624
                     /locus_tag="VFV99_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5059581.1"
                     /translation="ILASVCAAKLGDVKQAISTAETAVTYAEDEWQGTSVPSARQAAG
                     FEALLLYRHILALDVKLPERERVAAAFAAMEKARGRAYIDAAVRGGANLWSTSVEVPP
                     LLARDKKEIEAHVADLDKQIFAGKDDATQIERRRDALWALDDVKAAIAYRNPLITRLS
                     MPRPATLDRARDLLDDKSMLVAFLMTNDQAVAIAVTKTDARLYVIEGGPSALAAQVGV
                     LRDEAIGDPHASMELVHTKAHELYVKLLAPVDDLVKTHERLIVLPHGALSTLPLEALV
                     DNNGQFLVENHVVTYSQSATLALEDMRAKAKSPKRELVALGDPVYDWASFKAGKPEGL
                     PAAAARGMKRYSDAKSRGTRRGLERLPGTAAEVKAIAAMFGRDAKLYMRDEASEENVK
                     SGILGNSRIIHIASHGLFETDYQALALSMRPDAKDDGFLLQSEIAELKLDAELVVLSA
                     CETGRANEVLAEPVSGFVLALRTAGARRMLASLWPVDDTATMELMKAFYAPIVKSGAT
                     YASALTDAKRKLVATDKWKHPFFWAPFVLVGN"
     gene            1861..2637
                     /locus_tag="VFV99_09495"
     CDS             1861..2637
                     /locus_tag="VFV99_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059582.1"
                     /translation="MRSLLVALPLLAVACIGQISDPTDDGTGSGSGSGSGSGSGSGSD
                     ELPQARVSGSFDKSTLATELAKTEQLVLSITSENGFAGDVTLGARLVDAADAPLDGVT
                     VTGPTNITIAAGATEMATFELKVPMNALGAKFDGALKVDLMSTGATATVSSAVTIDAV
                     YTLDYLADTGVTAGKHTNAGNLPNLAVKRGAILRFKNDDLINHAIHGDGVFTNYHENT
                     VSGGAPGRTYEINTTGIMPGSGKIGCHDHGTATYSTYTIQ"
     gene            complement(2638..4311)
                     /locus_tag="VFV99_09500"
     CDS             complement(2638..4311)
                     /locus_tag="VFV99_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX5059583.1"
                     /translation="MRASLGQLFDRVIADPTSPDAPLLAQAVFAELERRARPTRVHRE
                     RLTKHAVLWWSLGFGPRGRAVLQRWRRGDPQNRELRLAETHFVVRTAPPQAAARFADV
                     RDAPAAALAVIHLVLAGEPHAALTHARAHRWFATRRDLAHVLMFIGWALGLTGSRDAE
                     RVLAMWKRHAAGDPEWLHHVLKAEAEIAMHACQYPRELQALRAAHALCVEHELGMQRV
                     AVEVTLPCAFAHNADLAAARAMMKRWGVPTDEYKPLDAGHDLARAEIELIAGRWDESE
                     KAARRALTFFTTADIAFHSCMAASFVALSAARPRFARALAEYRRIVHRVSVPFYRERY
                     QLLERLAARGLRSVRDAVFTERTRFARRPTTFAHVLFPPAESIAADVYWDRVQQRVWL
                     RGRGPYSLDDHPIVARMLETIVAADELTIPLATLFETVWRVPYQPLIHENKAHVTLHR
                     LRAWLDAHHKGMGRAVLVRDGIVSIAEDIEVVVLEPPTSLGEPETEQPPLIERVVALL
                     DEESPLTARELEQRLGISRTALNRATRTLLDAGRIACSGQGRALLYSVQ"
     gene            4480..5502
                     /locus_tag="VFV99_09505"
     CDS             4480..5502
                     /locus_tag="VFV99_09505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5059584.1"
                     /translation="MRVVLYAAITVGVLQGCVDDNLSVSTQGVIHGSLTPAGKYPATG
                     ALIAQVNGQRVVFCTGTLIAPDAVATAAHCMIPPPGIGDFTPYFTLALNANTAPASQL
                     VQGREKHYHPQYNQNVEAPGTLAHTYDVGIVLLTAPVPNAQIAYLPTPAEAATMMQGM
                     PLEITGYGLTIASDDNSAGVKYDATTTLSAVGQYEIGVGVAGGPQNCHGDSGGPAYMH
                     VGNGLRMVGLVSRGNTGGEDTCDRGGIDTRADAYRSWFGMYTTLADPPADPGPGEGSG
                     SGSGSGSGEGSGSGSGSGDDGTQENGSTYGGCSAGGAAGDFGTLALIALAVMSLCMRA
                     RLWRLV"
     gene            5644..6093
                     /locus_tag="VFV99_09510"
     CDS             5644..6093
                     /locus_tag="VFV99_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059585.1"
                     /translation="MEERLITGPPTRDSVERLDLFTLRLVTYAPGESAQRWITEELNS
                     LPCVPYFAASLRAAFEALAEEFRRRVIIVDYDALSKDELVELRMLRSRNTSGTFIALG
                     NVREHLRAPLRVTHVLPRPFGSEVLRNIVDELERQRDTQELLRLPRF"
     gene            complement(6130..6645)
                     /locus_tag="VFV99_09515"
     CDS             complement(6130..6645)
                     /locus_tag="VFV99_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5059586.1"
                     /translation="MPSRTDKIISKGMEKAKAVKATVKGIKGVFKTLMEQHGEVSGLL
                     RRVQKNTDKRTELWPQIRRELLSHERGELRVVFPVLRQFEETRHFAERHDADAATLES
                     QIQTLDEEDIDSAAWGEQFDTLVKLVTAHVNEEESEIFPAAMDVITGDRAEELDSQFM
                     QAKKAAMADMA"
     gene            complement(6749..6922)
                     /locus_tag="VFV99_09520"
     CDS             complement(6749..6922)
                     /locus_tag="VFV99_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059587.1"
                     /translation="MPDAILQVRERNMYSCGISADLLEPALDDLRRATFDRVGKWISL
                     TLLKFLVDVHHAS"
     gene            7163..7873
                     /locus_tag="VFV99_09525"
     CDS             7163..7873
                     /locus_tag="VFV99_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5059588.1"
                     /translation="MEASVQSYVDYRDYEARRVEAEHIGFLTHELKNPIGVVMMAKDQ
                     LCETATSPEQRKLCDLMDRSLGRLRTIIDEVLTAERLQAGAMEAHLVDLTLEDLFADW
                     LGALKRRAEAKHLALDLRFDPAVEIRADPNLTRLAVENLLDNAIKYSDEGTVSLEVDD
                     KPDEVLFHVRDQCGGLTPQQLAVIFEPFKRAHETKPGTGLGLSITRSAVEVHGGAIHV
                     ESSGMGCHFWFSLPKRHH"
     gene            7938..8135
                     /locus_tag="VFV99_09530"
     CDS             7938..8135
                     /locus_tag="VFV99_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5059589.1"
                     /translation="MNWDQVEGKWKELKGRVREKFGKLTDDDLEAIAGKKDQLVGRLQ
                     QRYGYNKEQAEREADDAMRSF"
     gene            complement(8141..8983)
                     /locus_tag="VFV99_09535"
     CDS             complement(8141..8983)
                     /locus_tag="VFV99_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059590.1"
                     /translation="MKNFAARHLAAIASLDGGLAQVKDPEFALLLRQFKSDHQESFAQ
                     VMALARLEHRNPPRGWRGANTLMKLEGRLAQLRGTTLTTRVLHFIEDQFTTAYREAEA
                     ESEGMRHQVLAALAAKAERRRAVLDAHLAPKPATFASLPARTCMRCLLDRPGAHPALT
                     RSNPHTYVCAACHDEVRTEFPEDLARQIESWPLQVRESRIITKALSRTSKQRAHLTVH
                     ARLAGQPETPETQAAPMRLPARAAQPPATPSTAPLVVERAPEGLETSYLERLFDYKKL
                     RSDW"
     gene            complement(8980..11172)
                     /locus_tag="VFV99_09540"
     CDS             complement(8980..11172)
                     /locus_tag="VFV99_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX5059591.1"
                     /translation="MRSTCGLCSIGCGVEVAVSDGKMCGIRGYDHPVSRGRLGPKGVG
                     QWQCNDHPTRGKTPLMRNQDRLVESTWDEAMDRIVDHVNRAIAEHGASAVAFYNSGQL
                     LLEEYYTLSKLARGGIGTPNVDSNTRLCTATVGESLMQSFGADGPPGAYEDFEQADCI
                     VLFGHNAAEQSTVLWMRMLAAKEANPALKIITIDPRRTATAKAADLHLAPRPGTNIAL
                     VNGIMRWLVENGHFDRDFVDEYTVGFAALRTLIEPYTPTHVEAITGIPAWQLARAAEW
                     IGTSPRVVTTCLQGVYQSNQATATACAVNTMHLVMGKVGKPGCAPFQFAGQPSSMNTR
                     ETGADGSYPGYRNWANPVHMADLAKRWNVPVEILGQKPIAAPDIFEQVVAGQVKVLFV
                     MGTNPAASLPDRERMLGGLRDVFLVVMDPFADTETVTLADVYLPTAMWGEKTGCMTNA
                     ERRCNLVQKVVEPPGLARSDFDILVDLARRMQLVDRSGRPLIGYTEPREAFEEWKACS
                     KGTIPDYSGMTYEMLVERGGVQWPCNSDRPNGTTRLYLDARFPTSPERAESYLKDLET
                     GHDHSKHEYEKVVPPRDRARLISEAYHPPGDEVNVEYPMIAVSGRLAYHWHTRTKTGK
                     VAELVDAAPRVYVGMHEEDAAGLHIEDGEVVRIVSRRGTLVAPAKIGDNTPRGVIFVP
                     FHFGDPETSPNSVMPALSDPASKQPIQKHAAVRVEKTDGRRDAWWRAP"
     gene            11645..12235
                     /locus_tag="VFV99_09545"
     CDS             11645..12235
                     /locus_tag="VFV99_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5059592.1"
                     /translation="MGRFHCPSCGESAYKHQKVRRWFTLFWIPMIPLDLAGEYVECGK
                     CYGTYDLAVLSYDPAAEKAKFEAVFHPAIRRTMVKMCLADGVVDPAEVEVIRDIYSKL
                     TGVELSEDDVREEIAEAMTDPKTVQQYLSAGIGMFNLKAKELILKSAFFVAAADGEFQ
                     EEEQKLMHNIGAALQMTKTQYQTVMDGLLGKDEAAA"
     gene            complement(12262..>12595)
                     /locus_tag="VFV99_09550"
     CDS             complement(12262..>12595)
                     /locus_tag="VFV99_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5059593.1"
                     /translation="VLIYGFNANALEFRTGWFVESLFTELVIALVVRTRRRSWQSRPA
                     PVLVWTTIGVAALAFALPYVPLSSELGFMSPPASLMLAVVGITAAYVVASELMKAWFY
                     RLPARARR"
BASE COUNT         2298 a         4117 c         4033 g         2147 t
ORIGIN      
        1 catcctcgcg tcggtctgcg cggccaagct cggggacgtc aagcaagcca tctccaccgc
       61 cgagactgcg gtcacgtacg ccgaggacga atggcagggc acgtcggtgc cgagcgcgcg
      121 ccaggccgcc ggcttcgagg cactgctgct ctatcgccac atcctcgcgc tcgacgtcaa
      181 gctacccgag cgcgagcgcg tcgccgcagc gttcgccgcc atggaaaagg cgcgcggccg
      241 cgcgtacatc gatgccgccg tgcgcggtgg cgccaacctg tggtcgacgt cggtcgaagt
      301 gccgccgctg ctcgcgcgcg acaagaagga gatcgaggcg catgtcgccg acctcgacaa
      361 acagatcttc gccggcaaag acgacgcgac gcagatcgaa cgccggcgcg acgcactgtg
      421 ggcgctcgac gacgtcaaag cggcgatcgc gtaccgcaat ccgttgatca cgcggctctc
      481 gatgccgcgt cccgcgacgc tcgaccgcgc gcgcgacctg ctcgacgaca aatcgatgtt
      541 ggtcgcgttc ttgatgacga atgaccaggc ggtcgcgatc gccgtcacga agaccgacgc
      601 gcgcctctac gtgatcgagg gcggcccctc tgcgctcgcg gcgcaggtcg gcgtgcttcg
      661 cgacgaagcg atcggcgatc cgcacgcgag catggagctc gtgcacacga aggcgcacga
      721 gctgtacgtg aagctgctcg cgccggtcga cgatctggtc aagacgcacg agcggctcat
      781 cgtgctgccg cacggcgcac tgtcgacgct gccgctcgaa gcgctcgtcg acaacaacgg
      841 ccaattcctc gtcgagaatc acgtcgtgac ctacagccaa tcggcaacac tcgcgctcga
      901 agacatgcgc gcgaaggcga agtcaccgaa gcgcgagctc gtcgctctcg gcgatccggt
      961 ctacgactgg gcatccttca aggcaggcaa acccgaaggc ctcccggctg ccgcggcgcg
     1021 cggcatgaaa cgctacagcg acgcgaagtc gcgtggcacg cggcgcggac tcgagcgctt
     1081 gcccggcacg gcggccgagg tcaaagcgat cgccgcgatg ttcggccgcg acgcgaagct
     1141 gtacatgcgc gacgaggcca gcgaggagaa cgtcaagtcc ggcatcctcg gcaactcgcg
     1201 catcatccac atcgcgagtc acggcctgtt cgagaccgat taccaagcac tcgcgctgtc
     1261 gatgcgcccc gacgcgaagg acgatggctt cctgctgcag agcgagatcg ccgagctcaa
     1321 gctcgacgcc gagctcgtcg tgctgagcgc atgcgaaacc ggccgcgcga acgaggtgct
     1381 cgccgagccg gtctcgggct tcgtgctcgc gctacgcacg gcaggcgcgc gccggatgct
     1441 cgcgagcctc tggccggtcg atgacaccgc gacgatggag ctgatgaagg cgttctacgc
     1501 gccgatcgtg aagtccggag cgacctacgc ctcggcgctc acagacgcga agcgcaaact
     1561 tgtcgcgacc gacaaatgga agcatccgtt cttctgggcg ccgttcgttc tggtcgggaa
     1621 ctgaaaccgc ccagacccct tttctacgag cgctggttaa tcggctccca tgttcgaaac
     1681 gtgcgcaacg tgccttacca agtgcgcacc aaacaaatct ccgtcgagaa cctgcaacgt
     1741 ggaggtaccg gcgccatgac tctccatgac tggggcagca gctcccaggg ctggtgtcca
     1801 cggtcccgtg tgtaatcggc ttgttatcgg cggcatcgca tttgctcatt gttgcgcaat
     1861 atgcgcagcc tcctcgtcgc ccttccgctc ctcgcagtcg catgcatcgg tcagatcagt
     1921 gacccgacgg atgacggtac aggctcgggg tcaggctcgg gttcaggctc gggctcaggc
     1981 tcgggctcgg atgagcttcc gcaggcgcgc gtgagcggca gcttcgacaa gtcgacgctg
     2041 gccaccgagc tggcgaagac tgaacagctg gtcctctcga tcacgagcga gaacggtttc
     2101 gcgggtgacg tcacgctcgg cgctcgcctc gtcgacgccg ccgacgcgcc gctcgatggc
     2161 gtgacggtga ccgggccgac gaacatcacg atcgccgcag gcgcgaccga gatggcgacg
     2221 ttcgagctca aggttccgat gaacgcgctc ggcgcgaagt tcgatggcgc gctgaaggtc
     2281 gacctgatga gcacgggcgc gacggcgacg gtgtcgtcgg cggtgacgat cgacgcggtc
     2341 tacacgctcg attacctcgc ggacacgggc gtcacggctg ggaagcatac gaacgccggc
     2401 aacctgccga atctcgcggt caagcgcggc gccatcctgc gcttcaagaa cgatgacctc
     2461 atcaaccacg cgatccacgg cgatggtgtg ttcacgaact atcacgagaa cacggtcagc
     2521 ggcggcgccc cgggtcgcac ctatgaaatc aacacgaccg gcatcatgcc cggctcgggc
     2581 aagatcggct gccacgatca cggcaccgcg acgtactcga cgtacacgat ccagtagtca
     2641 ctgcacgctg tagagcagcg cgcggccctg gcccgagcac gcgatgcggc ccgcgtcgag
     2701 cagcgtgcgc gtcgcgcgat tgagcgcggt gcgactgatg ccgagccgct gctccagctc
     2761 gcgcgcagtg agcggcgatt cctcatcgag tagcgcgacg acgcgctcga tcagcggcgg
     2821 ctgctcggtc tcgggttcac cgaggctcgt cggcggttcg agcacgacga cctcgatgtc
     2881 ctcggcgatc gacacgatgc cgtcgcgcac cagcaccgcg cgccccatgc ccttgtgatg
     2941 cgcatcgagc cacgcgcgta aacgatgcaa cgtgacatgc gccttgttct cgtggatcag
     3001 cggctgatac ggcacgcgcc acacggtctc gaatagagtc gcgagcggga tcgtgagctc
     3061 gtcggcagcg acgatcgtct cgagcatgcg cgcgacgatc ggatggtcgt cgagcgagta
     3121 cgggccgcgg ccgcgcagcc acacgcgttg ctgcacgcga tcccagtaga cgtccgccgc
     3181 gatcgattcg gcgggcggaa acagcacgtg cgcgaacgtc gtcggccggc gagcaaaacg
     3241 ggtgcgctcg gtgaacacgg catcgcgtac cgaacgcagg ccgcgtgccg cgaggcgctc
     3301 gagcagctgg tagcgttcgc gatagaacgg caccgagacg cgatgcacga tgcgacgata
     3361 ctcggcgagc gcacgtgcga atcgcggccg cgcagccgac agcgcgacga acgacgccgc
     3421 catgcacgaa tgaaacgcga tgtccgcggt cgtgaagaac gtcagcgcgc gacgcgctgc
     3481 tttctccgat tcgtcccagc gaccggcgat cagctcgatc tcggcgcggg cgagatcgtg
     3541 accagcatcg agcggcttgt actcgtcggt cggtactccc caccgcttca tcatcgcgcg
     3601 cgctgccgcc aggtcagcgt tgtgtgcgaa cgcgcatggc agcgtgacct cgacggcaac
     3661 gcgctgcatg ccgagctcgt gctcgacgca caacgcgtgc gcagcgcgaa gtgcctggag
     3721 ctcgcgtgga tactggcacg cgtgcatcgc gatctcggct tccgccttga gcacgtgatg
     3781 cagccactcg ggatcgcccg ccgcatggcg cttccacatc gcgagcacgc gctcggcatc
     3841 gcgcgagccg gtcaggccga gtgcccagcc gatgaacatg agcacgtgcg cgagatcgcg
     3901 acgcgttgca aaccagcgat gcgcgcgtgc gtgcgtgagt gcggcgtgcg gctcgccggc
     3961 gagcacgaga tggatgacgg cgagcgccgc ggccggtgca tcgcgaacat cggcaaaccg
     4021 cgcggcggct tgcggtggcg ccgtgcgcac gacgaagtgt gtctccgcga ggcgcagctc
     4081 gcgattctgc ggatccccac gacgccagcg ctgcagcact gcacgtccgc gcggcccgaa
     4141 gccgaggctc caccacagga cggcgtgctt ggtcagtcgc tcgcgatgca cgcgcgtcgg
     4201 tcgcgcgcga cgctcgagct cggcgaacac ggcttgcgcg agcagcggcg catctggact
     4261 ggtcggatcg gcgatcacgc gatcgaacag ctgaccgagt gacgcacgca tggtcatagc
     4321 aaaccgctgc acgcgcatgt cggcaagtaa cgcccgccgt cagagcctca cacgcgcgtg
     4381 ccgtcagaac ctcacacgcg agtaaggtca agaaagagcg cgtaacggga gctcgtgcgc
     4441 ccgcgcgtcc atcatctcct cgtcgggaag gagtgcgaga tgcgagtcgt tctgtacgca
     4501 gccatcaccg tcggagtcct gcagggttgt gtcgatgaca acctctcggt gtctacccag
     4561 ggtgtcattc atggatcgct cacgccggcc ggcaagtacc ccgcgaccgg tgcactaatc
     4621 gcgcaggtga acggtcagcg cgtcgtgttc tgtaccggca cgctgatcgc gcccgacgcg
     4681 gtcgcgaccg cggctcactg catgattcca ccgcccggca tcggcgactt cacgccgtac
     4741 ttcacgctcg cgctcaacgc gaacaccgcg ccggcatcgc agctcgtgca aggacgcgag
     4801 aagcattatc acccgcagta caaccagaac gtcgaggcgc cgggaacgct cgcgcatacc
     4861 tacgacgtcg gcatcgtgct gctcacggcg cctgtcccga acgcgcagat cgcatatctg
     4921 ccgacgcccg ccgaagccgc aacgatgatg cagggcatgc cgctcgagat cacgggctac
     4981 ggcttgacca tcgcgtccga tgacaacagc gcgggcgtga agtacgacgc gacgaccacg
     5041 ctgtccgcgg tcggtcagta cgaaatcggt gtcggcgttg cgggcggtcc acagaattgc
     5101 cacggcgatt ccggcgggcc ggcgtacatg cacgtcggca acgggctgcg catggtcggc
     5161 ctggtgtcgc gcggcaacac cggcggcgag gacacgtgcg atcgcggcgg catcgatacg
     5221 cgcgccgacg cctatcgcag ttggttcggc atgtacacga cgctcgcaga tccgccggcc
     5281 gatccgggac ccggcgaagg ctcgggctcg ggctcggggt cggggtcggg cgaaggctcc
     5341 gggtccgggt cgggatcggg cgacgacggc acgcaagaaa acggctcgac ctatggtggt
     5401 tgctccgcgg gcggcgccgc cggcgacttc ggcacgctcg cactcatcgc gcttgccgtg
     5461 atgtcgttgt gtatgcgcgc gcggctgtgg cgtctggtct agaggtaggt ctcgcgttct
     5521 gaaccgttcc tcgcgcgtaa gaatcgtgcg ctgctctaca gacctgacat tcgtaagtcc
     5581 gcgttacccg acgaatacct gcggaaaatt cgacgacagt gcacaattac gttcgaggga
     5641 tcgatggagg aacggctcat taccggcccg cccacgcgcg acagcgtgga gcgcctcgat
     5701 ctattcacgc tccgcctcgt tacgtatgcc cccggcgaat cagctcaacg ctggatcaca
     5761 gaagagctga attcgttgcc gtgcgtgccg tacttcgcgg cctcgctgcg cgccgcgttc
     5821 gaagcgctcg ccgaggaatt ccggcgccgc gtcatcatcg tggactacga cgcgctgtcg
     5881 aaagatgagc tcgtcgagct ccgcatgctt cgcagccgta acaccagcgg cacgttcatc
     5941 gcgctcggca acgtgagaga acatctgcgc gcgccacttc gcgtgactca cgtgctgccg
     6001 cgaccgttcg gcagcgaagt gctgcgcaac atcgtcgacg agctcgaacg ccaacgcgac
     6061 acgcaagagc tgctgcgact gccgcgcttc tgagcggacg acctctggga tcgcatcgcc
     6121 cggtaaccgt catgccatgt cggccatcgc cgccttcttg gcttgcatga actgcgaatc
     6181 gagctcctct gcgcggtcgc cggtgatgac atccatcgcc gccggaaaga tctcggattc
     6241 ctcttcgttc acgtgcgcgg tgacgagctt gacgagcgtg tcgaattgct cgccccacgc
     6301 tgcgctgtcg atgtcttcct cgtcgagggt ctggatctgt gattcgagcg tcgccgcatc
     6361 cgcatcatgc cgctcggcga agtgacgcgt ttcctcgaat tgacgaagca ccggaaagac
     6421 gacgcgtagc tcgccgcgct cgtgcgagag cagctcgcgc cggatctgtg gccatagctc
     6481 tgtgcgcttg tcggtgttct tctggacgcg acggagcaac ccggacacct caccgtgttg
     6541 ctccatcagc gtcttgaaca cgcccttgat tcccttcacg gttgctttga cggccttcgc
     6601 cttctccatc cccttcgaaa tgatcttgtc ggtacgactg ggcatcttct tattctcctt
     6661 ctgcccatgt cggatgcatc gcgcataccc ggggcgcgtg caggtatccg tcctgcaatg
     6721 gaactagctc gtctggcgcg tctgttgatc aggaggcatg gtgcacgtcg accaggaatt
     6781 tgaggagagt gagcgaaatc cacttgccga cgcgatcgaa cgtcgcgcga cggagatcgt
     6841 cgagcgctgg ctcgagcaag tcagcagaga tgccgcagct gtacatgttc cgctcacgga
     6901 cctgcaggat ggcatccggc actatttgga acgtttggcc gcgctccttc gtagtgagta
     6961 ctcgattgct accgtggcag cgtcggcgtg gagcgatatc gcgcgcgaac acgcactgac
     7021 gcgagtccgc ctgggatttg atgtcacaga gctcgtccgc gagatcgtta cgctgcgcac
     7081 gctgaccgct cagatacttc gcgacgaagg tttgctgaac aaagcagcaa tcgagctcct
     7141 gacaagcctc atcgacacgg caatggaagc gtccgttcag agctatgtcg attaccgcga
     7201 ctacgaagcg cgacgagtcg aggcggagca cattggcttc ctcacgcacg agctcaagaa
     7261 tccgatcggt gtggtgatga tggcgaagga ccagctttgc gaaacagcga cgtcgccaga
     7321 gcaacgcaag ctctgcgacc tgatggatcg cagtctcggt cggctccgaa ccatcatcga
     7381 cgaagtcctg acggcggaac gtcttcaagc aggtgcgatg gaagcccatc tggtcgactt
     7441 gacgctcgag gacctattcg cggattggct cggggcactc aaacgtcgag ccgaagccaa
     7501 gcacctcgcg ctggacctac ggttcgatcc agccgtcgag atccgcgccg atccgaacct
     7561 cacgcggtta gctgtcgaga acctgctcga caacgcgatc aagtactccg atgaaggaac
     7621 cgtgagcctc gaggtcgacg acaagcccga cgaagtccta tttcatgtcc gcgaccaatg
     7681 tggcggcctg acgccgcagc agctcgcggt catcttcgaa ccgttcaagc gtgcgcacga
     7741 gacgaagcca ggcaccggcc ttggtctgtc gatcacgcgc agcgcagtcg aagtgcatgg
     7801 cggcgcgatt catgtcgagt catcggggat gggctgtcat ttttggttct cgttgcccaa
     7861 gcgacatcat tgacggaagc ccgacgcaac ccggcacggc cgctgcaacg ctctacgtcc
     7921 gagaaaggag gcacgtcgtg aactgggatc aagtcgaagg aaaatggaaa gagctgaagg
     7981 gtcgcgtccg cgagaagttt ggaaagttaa cggacgatga cctcgaggcg atcgctggga
     8041 agaaggatca gctcgtgggc cggctccagc agcgctacgg ctacaacaaa gagcaagccg
     8101 agcgagaagc cgacgacgcg atgcgtagct tctgaccgga tcaccaatcc gagcggagct
     8161 ttttgtagtc gaacaggcgc tcgagatagc ttgtctccaa gccctcgggc gcgcgttcga
     8221 cgaccagcgg cgccgtactc ggtgtcgcgg gcggttgggc ggcgcgagcc ggtaaccgca
     8281 tcggtgccgc ctgcgtctcg ggcgtttccg gctggccagc gagacgcgcg tgcacggtca
     8341 ggtgcgcacg ctgctttgac gtccgcgaca acgccttcgt gatgatgcgg ctctcgcgaa
     8401 cctgtagcgg ccacgactcg atctgccgcg cgaggtcttc gggaaactcg gtgcgcactt
     8461 cgtcgtgaca cgctgcgcac acataggtgt ggggattgga gcgggtcagc gccgggtgtg
     8521 cgcccgggcg atcgagcaag catcgcatgc aggtgcgcgc cggcagcgac gcaaacgtcg
     8581 ccggtttcgg tgcgagatgc gcgtcgagca ccgcgcgacg tcgttcggcc tttgcggcga
     8641 gcgcggcgag aacctgatga cgcatcccct cggactcggc ctccgcctcg cgatacgcgg
     8701 tcgtgaactg gtcctcgatg aaatgaagca cgcgcgtggt caacgtcgtt ccgcgcagct
     8761 gcgcgagccg gccttcgagc ttcatcagcg tattcgcgcc acgccaacct cggggcggat
     8821 tgcgatgctc gagccgcgcg agcgccatca cctgcgcgaa tgactcctga tgatcggact
     8881 tgaactgccg cagcaggagc gcgaactcgg ggtccttgac ctgagcgagg ccgccgtcga
     8941 gcgacgcgat tgcagccaga tggcgtgcag caaaattctt catggcgccc tccaccacgc
     9001 atcgcgacgg ccatcggtct tctcgacgcg aacggcggcg tgtttctgga tcggttgctt
     9061 ggaagcgggg tcggatagcg cgggcatcac gctgttgggt gacgtctccg gatcgccgaa
     9121 gtgaaacggc acgaagatca cgccgcgcgg cgtgttgtcc ccgatcttcg ccggcgcgac
     9181 gagcgtcccg cgtctcgaca cgatgcgcac gacctcgccg tcctcgatgt gaagcccggc
     9241 ggcatcctcc tcgtgcattc cgacatacac gcgcggcgcg gcgtcgacga gctctgcgac
     9301 cttgccggtc ttcgtgcgcg tgtgccagtg gtacgccaac cgaccgctca ccgcgatcat
     9361 cggatactcg acgttcacct cgtcacccgg cggatgatac gcctcgctga tcagtcgcgc
     9421 ccgatcacgc ggcggcacca ccttctcgta ctcgtgcttc gagtggtcgt gaccggtttc
     9481 gagatccttg agatagctct cggcacgctc gggtgaggtc gggaagcgcg catcgagata
     9541 gagccgcgtc gtgccattcg ggcgatcgct gttgcacggc cactgcacgc cgccgcgttc
     9601 gaccagcatc tcgtacgtca tgccggagta atcgggaatc gtgcctttcg agcacgcttt
     9661 ccactcctcg aatgcctcgc gtggctcggt gtaaccgatc agcgggcgcc ccgagcgatc
     9721 gaccagctgc atgcgcctgg ccaagtcgac gaggatgtcg aagtcggagc gcgcgagccc
     9781 gggcggctcg acgaccttct ggacgaggtt gcagcgacgc tctgcattcg tcatgcagcc
     9841 cgtcttttcg ccccacatcg cggtcggcag atatacatcc gcgagcgtca cggtctcggt
     9901 atccgcgaac ggatccatca cgaccagaaa cacgtcgcgc aaaccgccga gcatgcgctc
     9961 gcgatccggc aggctggccg ctggattcgt acccatcacg aacaacacct tcacctgccc
    10021 cgccacgact tgctcgaaga tgtcgggcgc cgcgatcggc ttctggccga ggatctcgac
    10081 gggaacgttc cagcgcttcg cgagatccgc catgtgcacc gggttcgccc agttgcggta
    10141 gccgggatag gagccgtccg cgccggtctc gcgcgtgttc atcgagcttg gctgccccgc
    10201 gaactgaaat ggcgcgcacc cgggcttgcc gaccttcccc atcaccaagt gcatcgtgtt
    10261 gaccgcgcac gcggtggccg ttgcctggtt cgattgatag acgccttgca gacaggtggt
    10321 gacgacccgc ggcgacgtgc cgatccattc cgcggcacgc gcgagttgcc acgcggggat
    10381 tccggttatc gcttcgacgt gcgtaggcgt gtatggctcg atcaacgtgc gaagcgcggc
    10441 gaatccgacc gtgtactcgt cgacgaagtc gcgatcgaaa tgaccgttct cgaccagcca
    10501 ccgcatgatg ccgttgacga gcgcaatgtt cgtcccgggt cgtggcgcca ggtgcagatc
    10561 ggcggccttc gccgtcgccg tgcgccgagg atcgatcgtg atgatcttca gcgcgggatt
    10621 cgcttccttc gcagcgagca tgcgcatcca cagcaccgtg ctctgctcgg cggcattgtg
    10681 gccgaacagc acgatacagt ccgcttgctc gaagtcctcg taggcgcccg gtggaccatc
    10741 ggcaccaaag ctctgcatga gtgattcgcc aaccgtcgcg gtgcacagcc gcgtattcga
    10801 gtcgacgttc ggcgtgccga tgccgccacg cgcgagcttc gacagcgtgt agtactcctc
    10861 gagcagcagc tgtccggagt tgtaaaacgc gactgccgat gcgccatgct cggcgatcgc
    10921 gcgattcacg tgatcgacga tgcggtccat tgcctcgtcc cacgtgctct cgacaaggcg
    10981 atcctggttg cgcatcagcg gcgtcttgcc gcgcgttgga tggtcgttgc actgccactg
    11041 cccgaccccc ttcggaccca gacgaccacg actcaccgga tggtcgtagc cgcgaatgcc
    11101 gcacatcttg ccgtcgctaa ccgcgacctc gacgccgcat ccgatcgagc acaagccgca
    11161 ggtagaacgc acccaccgta cgttcgagtt ttccggaatc cggccgacca aacgcagtct
    11221 cattgcgacc gctgatgcaa acgctgtgta ccgggcagac cgtggtttca cccgaatgtg
    11281 tagttgcttc gtgacgcgct cgctcagcgt gcggcagcgc gcgggcaatc atcgacacag
    11341 tgtctggctt gcgccgtaat gcgacgatcg ttgcggcgga catcgcgaga accgccgtcg
    11401 gccgcgccat tttcgtaaac acgtcagaca tcgatcgaaa tgaccgcaaa ccgcgtgcac
    11461 gagcgcatgc gccgttcgct aacaggctcg cccgcacggt ccccgcgtga gggctcgagc
    11521 ccgcacgcgg acccgaaagt tgcgggatgc gcgccgcact cccgcgcgct cgtgatgaga
    11581 tagagacgcg atgctctact tcatcattgt ttacggaacg cgaggtatga cgtccacggg
    11641 cgggatggga cgcttccact gtccctcgtg cggcgagagc gcatacaaac atcagaaggt
    11701 tcgtcgctgg ttcacgctgt tctggattcc gatgatcccg ctcgatctcg ccggcgagta
    11761 tgtcgagtgc ggcaagtgct acggcaccta cgacctcgcc gtcctctcgt acgatccggc
    11821 ggccgagaag gcgaagttcg aggccgtgtt tcaccccgcg atccgccgca cgatggtgaa
    11881 gatgtgtctc gcggacggcg tcgtcgatcc cgcggaggtc gaagtgatcc gcgatatcta
    11941 ctcgaagctc acgggcgtcg agctttccga ggacgacgtg cgcgaagaga tcgcggaggc
    12001 gatgaccgac ccgaagaccg tccagcagta cctctcggcg ggcatcggca tgttcaatct
    12061 caaggcgaag gagctgatcc tcaagtcagc gttcttcgtt gcagctgccg atggcgagtt
    12121 ccaggaagag gaacagaagc tgatgcacaa catcggcgcg gcgctgcaga tgacgaagac
    12181 gcaatatcag accgtgatgg acggcttgct cggcaaagac gaagccgccg cctgaccgcg
    12241 tgccgcgcta gctagatccg atcatcgcct cgctcgtgct gggaggcggt agaaccacgc
    12301 cttcatgagc tcgctggcga ccacgtacgc cgccgtgatg ccgaccacgg ccagcatcaa
    12361 cgacgccggt ggagacatga acccgagctc tgaagacagc ggcacgtacg gcaatgcaaa
    12421 ggcgagtgcg gcgacgccaa tcgtggtcca cacgagcacc ggtgccggtc ggctctgcca
    12481 gctcctccgc cgtgtgcgga cgacgagcgc gatcacgagc tcggtgaaca acgactcgac
    12541 gaaccagcca gtccggaact cgagcgcgtt cgcgttgaag ccgtagatca gcacg
//
LOCUS       DASUFX010000244        12981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_176034,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000244 DASUFX010000000
VERSION     DASUFX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176034"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFV99_09555"
     CDS             <1..505
                     /locus_tag="VFV99_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5059594.1"
                     /translation="TMTMENVGTFAVTADPTGAVFAPWNSARPGEDVERQGMPKPFTF
                     CWDELMSTDPAVAGAFYEKVFGWKSRPVDMGGGMIYTLLDRPGVKNAKGDQQSAGGMM
                     KSPPGVPYSFWLAYVAVDNCDKLCERATRLGAKVTVGPTDIPNVGRFACWFDPQQAAI
                     AILQPQM"
     gene            complement(509..1006)
                     /locus_tag="VFV99_09560"
     CDS             complement(509..1006)
                     /locus_tag="VFV99_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059595.1"
                     /translation="MMRLAVILAPLLLLACGSHGPHDGGQIDDAASSPDPDGQQQPGD
                     DGAMVMVDAAIDAKPMPDAFPGVLDVRIDCHNDCVLHATPSMIAVSAGTSFQVNWINV
                     GDTDCDVAKIDQFNQVPIIIGLEPGTSYHDPVHDWCGTQFTGTFNFRISICTIPSYIP
                     VDCGA"
     gene            1205..1693
                     /locus_tag="VFV99_09565"
     CDS             1205..1693
                     /locus_tag="VFV99_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059596.1"
                     /translation="MKRACLLLAVGCTAEPRTATDYAPLTTWAQAADFRLSQPQHCNF
                     EEQHFGATFGTCWFAKSRHAVPAGSTMFPRMDITLAEFPSETIAIERIKHFREKPVGA
                     GLLPEEDKAFPLRAGVRIGSRVLILATDAYAFEPDIYRAAGTLAAATHGELVCWSPPC
                     TR"
     gene            1770..2396
                     /locus_tag="VFV99_09570"
     CDS             1770..2396
                     /locus_tag="VFV99_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059597.1"
                     /translation="MMTKQEQAVRKRFQRAIGRHRRARTAASWFSLGTILAEAYGVSH
                     ELGHELCEGVGDPITCWRHCVRLEPEHAEAWHRLGQAYFELDDRTKAVRALTRAVKLD
                     PHVPSAWNSLGILTLPAPEEDAPARLRRAEGFLRRAIEEDRRGKKLGWEPFAWLAEAA
                     ERRRHDPDALAWYSEANKRGDRYAAARKQVIENHGARRRGAPRPSNAK"
     gene            2516..3706
                     /locus_tag="VFV99_09575"
     CDS             2516..3706
                     /locus_tag="VFV99_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5059598.1"
                     /translation="MRRVLLVTLAACGGYDDPSDTMDVCGLAETASYPSGPNPSGLVA
                     DDLNGDAELDFMIPSEGTQLHIFYGTGNGAFTGAEVPAGDRPTAIAVGDFTEDGRADI
                     AVSNLSTVPSTPTGVSILERRADGSFARLPQLLPIAAFHVVATDFDNDGHLDVVASST
                     TGVRVFPGTGMGTFAINAPELMLGQTPTFKIADVDGDGTRDLIALSAFKLVVRRSGTT
                     MATSTTNMYDTGGVYPRDLAIGDLDADGDLDLAVANGDNDTVRLFFNTGGSFQTQVDL
                     PTAIPPGSDGASMGSGSGSGSNIGSWLPRNVAIGDFTGDGTSDVLVLIEPYLPTTDAS
                     TMLVLERTANGGYQQRPCAMATRPVGVVAGNFFGDARTDAIIVTAPYDARSGGTVMLV
                     QQTP"
     gene            complement(3709..4992)
                     /locus_tag="VFV99_09580"
     CDS             complement(3709..4992)
                     /locus_tag="VFV99_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEX5059599.1"
                     /translation="MRSLTCAAFAVLAAASPAFAKSVSGPGPTHTSMDPGPVTLTTAQ
                     RKARAEIIRDTAAGLGMTNAALLAGIGEVETNFAHCWSEATWACQGPASSSCNGGPVI
                     AGSADGPCADQQGGLGMFQFDSGTYQQTINTYGPNIVTMQGNVDAVVPFLVTRAIESV
                     DGVHNQAEALAWMNSITIQDGDPQYEKWLYFVSWRYNGCKGCTTQQNKYRAGTNKLRD
                     EFGADFWKVSTEPVTCEKISATDGRVIDDKDGCFVKGGSPASWYPSTKGTAGACLFTY
                     TTDAAAEDNNATWKLDFAVDGTYRIEVNTDSSVAQTHQARYVVTHAGGTTDVVIDQSA
                     TTGYQSLGEFEFVAGEPASVHLGDNTGETYVSTAKIAIMFDSVKVTPVAAGQGSGSDD
                     GGDDDPGDGGGCSTSGSGSLVILVALGALVRRRRS"
     gene            5168..6136
                     /locus_tag="VFV99_09585"
     CDS             5168..6136
                     /locus_tag="VFV99_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059600.1"
                     /translation="MRAPGVIGVLVDQGALAADDPGVAQLAALAGGITAFGSRLDQIG
                     WWCIRDAQIALRAVSAIGDAPAPLADAMRSIRTALAGRGGSLVTATAQAACATLLATP
                     GADVDAALVLVTHLYGEAVATECRPFAASLDRTALAHAHLDAIWALALAARGAGSGWT
                     AYHAHRRLPSDIATHAARAVVAASTHLGRPLAPDALAWALELAAAPLRVRLVYPESAV
                     AAVSTLREQLAHHAIDECRIAAYPTGDPIVRFCHRALVDCDAVVAVVTAGDNSVWLRR
                     ELAVQYELFGEIRPRVILALAGARPESSELATAELSNIADIVQHLR"
     gene            6197..7507
                     /locus_tag="VFV99_09590"
     CDS             6197..7507
                     /locus_tag="VFV99_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059601.1"
                     /translation="MRNHRMRLALALLALAAFVAGCSDEDVGTSPVTGTVVFTERATQ
                     HIRVLDVGTGVSDLIDGGQFGSVSIAPDAAHVAYQGVDQIMKFADRSANVTPVLPGGG
                     CVGPGTWLTSRSLAYCATINGNSGFMLLPDLDGTAPRFIGPLPAISNDGHQLSYVDAR
                     GDAVIEDIDGANRRVVVPSTDPTALYHFRGVTGFTPDDRAILISDYEMYPAKLRIVAL
                     ADGSSIDVDDALDGSSPFGPTLFYGASRFSPDGSEIVLQSTTGLVAIDLGTGTKRVLA
                     PFAPRTSSAGAVFLDAEHVLWVQVENHSVSDIGKYTASLHVAGPTVEDDRVLDAPNDE
                     NALYPSVAVSPEGFIAWPGNAFMMTMAGTVLVENDYSSPSVYEDILGLTPNGRGVIAI
                     TYDGLVRYVDRSGASRDLATVGGTGDLLAPYAAYTPASTASRRH"
     gene            complement(7504..8400)
                     /locus_tag="VFV99_09595"
     CDS             complement(7504..8400)
                     /locus_tag="VFV99_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX5059602.1"
                     /translation="MLRLATRAPFHLEATVRVLQRRPTNLVERWEQNRYIRVITTRAG
                     LSVVEVENRGTIDAPNVHFSISHGSASKASRTEIERILRVKLGLDVDPRPLQRRAEAV
                     PALRTTARALRGMRPPRFADLFEAFANVIPFQQVSLDAGIAIVTRLVERFGLVLEHAG
                     TRRYAFPTASTIASARLEALRRCGLSGHKAIALKNCARLIASGKLTDEAIAAASTEQA
                     LAILAALPGIGPWSAALVLLRGFGRLDVFPPGDVGVARNVGRIASSLAPIVERFGPIR
                     GYLYFCLLGGALLERGLIKAAR"
     gene            complement(8414..10027)
                     /locus_tag="VFV99_09600"
     CDS             complement(8414..10027)
                     /locus_tag="VFV99_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5059603.1"
                     /translation="METTTDVLVVGGGPTGLMMATQLARYGVGIRIVDEQADRAQESR
                     AFGLQARSLEIFDQLGLGEEFASRALRAEPIRAYVRGRRIAELHFDPLVGITRYPGLY
                     LLAQPETERILLDDLSARGIAVERSSRLVSFVQDPDGVTAELAHIGSAARETVRCRYL
                     VGCDGAHSRVREVLEIPFEGSTYPAEFMLADVELPRDVPFAVYITRHAVCLVGVLGDR
                     TRIMGVVVDQPSPAADAPVTIDDINALTRAARAPIQIDSALWTSRFRLHHRATRRYCV
                     GRAFLAGDACHIHTPFGGQGMNTGFGDATNLAWKLAFVLRGASRELLDTYELERERVG
                     EVLVRTTDRAFGLATSHRFGAGVLRELIAPIVLQAVARSYRLRSRVVRFASQLDIRYH
                     PSYAVREVSDDADPTFRSALQAGCRVPDLDLDGISLHDLLRGTAVHVLAFGPCKEGEL
                     RDVEHRHAGTIVVHRVQATAGAEEVFQRFGVSQAAIYIVRPDGYIGFRSFGPSAHDAA
                     DYLNAVLSPAPWQVTSARPTTQPRADRTS"
     gene            complement(10116..>12981)
                     /locus_tag="VFV99_09605"
     CDS             complement(10116..>12981)
                     /locus_tag="VFV99_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX5059604.1"
                     /translation="TSSDAVRLVIAEDGRVLAERNLVADAAFSYRVFAESTGEMHPFD
                     GPIVDSTPHATGHPNGAFPAYATSNLVTVDGLNHPGTGPADPWLVNNRTETLGNNVEA
                     YADFNAPDGLTFGDFRATTTAAGKFDRAYDFNAEPLSTQTQQMTGITSLFYTVNWMHD
                     FWYDGGFTESAGNAQDNNYGRGGEDRDAIKAEAQDNANGGSRNNANMSTPDDGLQPRM
                     QVFLWGGKETRTLTIATHTTTSGSASFGPKNFDVTGEVVVADDGVGTGTDACTALPSL
                     TGKIVLVDRGTCTFKTKTLNVQTAGGIGVILANNAASTSPPGLADDATITTAITIPTL
                     SILQTDGTQLKTDIAAGAVNVTMQREVGVELEGTLDTSVIAHEFGHYVHHRLSVCGTS
                     ACGAMSEGWGDFSSLLVVARDGDNLMGAYPMGIYSTQSFSADPAYFGIRRAPYSADHT
                     INDLSFRHMTDGVATPTTHPFNASGNNAEVHNAGEVWAAMLWEGYVALQGAGTSFDDV
                     RLKMRQYVVAGLLLAPPDATPTETRDAILTAARAASMADHDTLVTAYARRGFGSCAVS
                     APRSSSNFMGIVESNVVKGNAVLGAAQMALATSCDTDEVLDAGETAKVTLPIANTGPT
                     ALTNVVATLTTTTDGVTVASGPVSIGTIAPYGSAMASFDVTVADSIVDPTAAAFTMTL
                     TTDDGCATTIEQPLAVAINTDDKVASSATDSFDAMASVWTPPSTDSQWSHTRPTPLDG
                     IWFANDSGARTDSSLTSPALTADATAPVSITFSHKFSFEFSSNTYWDGGVIEISTDNG
                     ATWQDVATLATASPYNSTLTTTSDNPLGGRAAFGNKNASYPNPDSVTLDLGTMLAGKT
                     FQLRFRVVTDGGTGGAGWEINDVAFTGIVGTPFPTLVPDAGECAGPGPDGGDMPDGGT
                     DNPETGDSDGGCCQSQRSPAGSGVLGLSVLALIVRRRRRR"
BASE COUNT         2173 a         4257 c         4332 g         2219 t
ORIGIN      
        1 gacgatgacg atggagaacg tcggcacgtt cgcggtcacc gccgatccga ccggcgccgt
       61 gttcgcgccc tggaacagcg cgcggcctgg cgaagacgtc gagcggcagg gcatgccgaa
      121 gccgttcacg ttctgctggg acgagctgat gtcgacggac ccggcggtcg cgggtgcgtt
      181 ctacgagaag gtcttcggct ggaagtcgcg gccggtcgac atgggcggtg gcatgatcta
      241 cacgttgctc gatcggccgg gcgtgaagaa cgcgaagggc gatcagcaat ccgctggcgg
      301 gatgatgaag tcgccgcccg gcgtgccgta ctcgttctgg ctcgcgtacg tcgccgtcga
      361 caactgcgac aagctctgcg agcgcgcgac gcgtctcggc gcgaaggtca ccgtggggcc
      421 gacggacatt ccgaacgtcg gccggttcgc atgctggttc gatccgcagc aggccgcgat
      481 cgcgatcctg cagccgcaga tgtagccgtc acgcgccgca gtcgacgggg atgtacgacg
      541 gaatcgtgca gatcgagatg cggaagttga acgtgcccgt gaactgcgtc ccgcaccagt
      601 cgtgcacggg atcgtgatag ctcgttcccg gctcgagacc gatgatgatc ggaacctggt
      661 tgaactgatc gatcttcgcg acatcgcagt cggtgtcgcc gacgttgatc cagttgacct
      721 ggaacgacgt ccccgcggaa accgcgatca tcgatggcgt cgcgtgaagc acgcagtcgt
      781 tgtggcaatc gatgcggacg tcgaggacgc ccgggaacgc atcgggcatc ggcttcgcat
      841 cgatggcggc atcgaccatg accatcgcgc cgtcgtcgcc gggctgctgc tggccgtcgg
      901 ggtccgggct cgacgccgca tcgtcgatct ggccgccgtc gtgtggtcca tgggacccgc
      961 acgcgagcag aagcagtggt gccaggatga cggcgagacg catcacttgg gtggagcctc
     1021 gggcgccggg gaagtcgcac gaaaagatgc gtccactgga acacggccgg ctcccggttg
     1081 gcggcgagtg cgatgtcggc attgacctgt cgcgcctgcc aagctggcag cccgtgggcg
     1141 tgggatccgc tgtcaacatg gcagtgaagc ggaacagtag gcgacatcgc cgcgttacgt
     1201 cgacatgaaa cgggcctgcc tccttcttgc cgtgggttgc accgccgagc cgcggactgc
     1261 caccgactac gcgccgctca cgacgtgggc gcaagccgcc gacttcaggc tctcgcagcc
     1321 acagcactgc aacttcgagg agcagcactt cggcgcgacg ttcggtacgt gctggttcgc
     1381 gaaatcgcgc cacgcggtcc ccgccggctc gacgatgttt ccgcgcatgg acatcacgct
     1441 agcggaattt ccgagtgaga cgatcgcgat cgagcgcatc aagcactttc gcgagaagcc
     1501 ggtgggcgca ggcctgctcc ccgaggagga caaggcgttc cccctgcgtg cgggagttcg
     1561 cattggctcg cgcgtcctga tcctggcgac cgacgcgtac gcgttcgagc ccgatatcta
     1621 tcgggcggcg ggcacgctcg cggcggcgac ccacggcgag ctcgtatgct ggtcgccgcc
     1681 ctgcacgcgt tgacccgcgc gaagccactt cacgcgagat cgtgcccgca acagaacgcg
     1741 cctcgcagga tgcgatacct tcgcggggga tgatgacaaa gcaggagcag gctgtacgca
     1801 agcggttcca gcgggcgatc ggtcgccatc gtcgtgcacg aacggccgca tcgtggttct
     1861 cgctcggcac gatcctcgcc gaggcatacg gtgtctcgca cgagctcggt cacgagctgt
     1921 gcgaaggcgt cggcgatccg atcacgtgct ggcggcactg cgtccgactc gagccggagc
     1981 atgccgaggc gtggcaccgg ctcggccagg cgtacttcga gctcgacgac cggacgaaag
     2041 ccgtgcgcgc gctgactcgc gcggtgaagc tcgatccgca cgttccgtcg gcatggaaca
     2101 gcctcggaat cctcacgctg ccggcaccag aagaagacgc gcccgcacga ctgcgccgcg
     2161 ccgaggggtt cctgcgacga gcgatcgaag aggatcgccg cggcaagaag ctcggctggg
     2221 agccattcgc gtggctcgcg gaagcagccg aacgccgtcg ccacgatcca gatgcgcttg
     2281 cttggtattc cgaagcgaac aagcggggcg atcgctatgc ggccgcgcgc aagcaggtca
     2341 tcgaaaacca cggcgctcgg cgtcgcggcg cacctcgccc gtcgaacgcc aagtaggcgg
     2401 gcgccgtccg tagcgtcgcc gcaacaacgc gctgcacgtt accagcgtca caccccggcg
     2461 gccgctcggt attttggaca gctcgccgtg ctgcccgacg cggtacgctt taaccgtgcg
     2521 tcgcgtcctg ctcgtgacac tcgccgcgtg cggcggatac gacgatccga gtgacacgat
     2581 ggacgtctgc gggttggcgg agactgcgtc gtatccgagc ggcccgaatc cgagcggatt
     2641 ggtcgccgac gatctgaacg gagacgccga gctcgacttc atgatcccga gcgaaggcac
     2701 gcagctgcat attttctacg gcacgggcaa cggtgcgttc accggggcgg aggttccagc
     2761 aggtgaccgt cccacggcaa tcgcggtcgg cgatttcacc gaggacggtc gcgccgacat
     2821 cgcggtgtcg aacttgtcga ccgtgccgtc gacgccgacc ggcgtctcga ttctcgaacg
     2881 ccgtgccgat ggatcgtttg cacggttgcc ccagttactg ccgatcgcgg cgttccacgt
     2941 cgtcgcgact gacttcgata acgacgggca cctggacgtg gtggcgtcgt cgacgaccgg
     3001 tgtgcgcgtg tttcccggca ccggcatggg cacgttcgcg atcaatgccc ccgagctgat
     3061 gctcgggcaa acgccgacat tcaagattgc ggatgtcgac ggggatggca cgcgtgattt
     3121 gatcgcgctc tcggcgttca agctggtcgt gcgcaggagt ggcacgacga tggcgacgtc
     3181 gacgacgaac atgtacgaca ccggcggcgt ctacccgcgc gacctcgcaa tcggcgatct
     3241 cgatgcggac ggtgatctcg atctcgcagt cgcgaatggc gacaacgaca cggttcgcct
     3301 gttcttcaat accggtggtt cgttccagac ccaggtcgat ctaccgaccg ccatcccgcc
     3361 tggcagcgac ggagcgagca tgggatctgg atccggatcc ggatcgaaca tcggctcgtg
     3421 gcttccgcgc aacgtcgcga tcggcgactt cacgggtgac ggcacgagcg acgtactggt
     3481 gttgatcgag ccttacctgc cgaccacaga cgcgtcgacg atgctcgtgc tcgagcgaac
     3541 cgcgaacggc ggataccagc agcgtccgtg cgcgatggcg acgcgacccg tcggtgtcgt
     3601 cgccgggaac tttttcggtg acgcgcgcac cgacgcgatc attgttacgg cgccgtacga
     3661 tgcgagatcg ggcggcacgg tcatgctcgt gcagcagacg ccgtagcgtt acgagcggcg
     3721 ccggcgcacc aacgcgccaa gcgcaaccag gatgacgagc gagccactgc ccgaggtcga
     3781 gcatccgccg ccgtcgccag gatcgtcgtc gccgccatca tcactgccgc tgccctgccc
     3841 tgcggcgact ggcgtgactt tgacgctatc gaacataatg gcgatcttcg ccgtcgagac
     3901 gtacgtctcg cccgtgttgt cgccgaggtg gaccgatgcc ggctcgccag cgacgaactc
     3961 gaactcgccg agcgattgat agcccgtcgt cgcggactga tcgatcacga cgtcggtcgt
     4021 accgcccgca tgcgtgacga cgtagcgcgc ttggtgcgtc tgcgcgacgg acgagtcggt
     4081 gttgacctcg atgcgatatg tgccatcgac ggcgaagtcg agcttccagg tcgcgttgtt
     4141 gtcctcggcg gcggcgtcgg tcgtgtacgt gaacaagcac gcaccggcgg tgcccttcgt
     4201 gctcgggtac cacgacgctg gcgagccgcc cttgacgaag cagccgtcct tgtcgtcgat
     4261 gacgcgcccg tcggttgccg aaatcttctc gcacgtgacc ggctcggtgc tcaccttcca
     4321 gaagtcagcg ccgaactcgt cgcgcagctt gttcgtgccg gcgcggtact tgttctgctg
     4381 cgtcgtgcag cccttgcagc cgttgtagcg ccacgacacg aagtacagcc atttctcgta
     4441 ctgcgggtcg ccgtcttgga tcgtgatcga gttcatccac gcgagcgcct cggcttggtt
     4501 gtggacgcca tccaccgatt cgatcgcgcg cgtgacgagg aacggcacga ctgcgtcgac
     4561 gttgccttgc atcgtgacga tgttcgggcc gtaggtgttg atcgtctgct gatacgtgcc
     4621 ggagtcgaac tggaacatgc cgaggccacc ctgctgatcc gcacacggcc catcggccga
     4681 gccagcgatc accggaccgc cgttacacga cgacgacgcg ggaccctgac atgcccacgt
     4741 cgcttcggac caacagtgcg cgaagttcgt ctcgacctca ccgatgccgg cgagcaacgc
     4801 ggcgttcgtc atgccgagcc ccgcagcggt atcgcgaatg atctcggcgc gcgctttccg
     4861 ctgtgcagtc gtgagcgtca ccggacccgg atccatgctc gtgtgagtag gaccgggacc
     4921 tgagaccgat ttggcaaacg cgggcgatgc ggcagcaagc accgcgaacg cggcgcacgt
     4981 gaggcttctc atcgcgtgcg cgtaacttca tcgatcgtgc cgaacacgag ccgcagtatt
     5041 tcgcgccggt agcgcgctgc gctcatccga acgcgcgaca gtgcaacctc gattgtgcag
     5101 ccatctctgc acaggacaca tgtaggcctt ggcgttgcgc cggacaggcg gtgatagcct
     5161 cggtgacatg agagcgcccg gcgtgatcgg cgtactcgtc gatcaaggtg cgcttgcggc
     5221 cgacgatccg ggcgtcgcgc agctcgcggc actcgcgggc ggcatcacag cgtttggctc
     5281 gcgactcgat cagatcggct ggtggtgcat acgcgatgcg cagatcgcgt tgcgcgcggt
     5341 gtccgcgatc ggcgacgcgc cggccccact tgcggacgcg atgcggtcga tccgcaccgc
     5401 gctcgccggt cgcggtggct ctctggttac tgcgactgcg caagcggcat gcgcgaccct
     5461 gctcgcgaca ccgggggccg acgtcgacgc tgcgctcgta ctcgtcactc atctctacgg
     5521 cgaagccgtg gccaccgaat gtcggccgtt cgctgcatcg ctcgatcgca cagcgcttgc
     5581 ccacgcccac ctcgacgcga tctgggcgct cgcattagcc gcgcgaggag ccggctcggg
     5641 atggaccgcg taccatgcac atcgccgtct gccgtccgac atcgccacgc acgcggcgcg
     5701 cgccgtggtc gccgcgtcga cgcacctggg ccgtccgctc gcgcctgatg ctctcgcctg
     5761 ggcactcgag ctcgcagccg ctccactacg cgtgcggctg gtgtatcccg agtccgccgt
     5821 tgccgcagtg agcacgctgc gcgagcaact tgctcaccac gcgatcgacg agtgccgcat
     5881 cgcggcctat ccgacgggcg acccgatcgt ccggttctgc catcgcgcgc tcgtcgactg
     5941 cgatgccgtc gtcgccgtcg tcaccgccgg agacaactcg gtctggttgc gccgcgagct
     6001 ggcggtccag tacgagctgt tcggcgagat ccggccgcgc gtgatcctgg cgctcgccgg
     6061 agcccgccca gagtccagtg agcttgcgac tgccgagctc tcgaacattg ccgacatcgt
     6121 gcagcacctg cgctagcgct cggttcgtgg tccggatcac gcgtcccgcg cgggcgcaaa
     6181 gcggtacgct tggatgatgc gcaaccatcg gatgcggctc gcgctggcgc tgctggcctt
     6241 ggctgccttc gtcgccggct gttctgacga ggacgtcggg accagccccg tgaccggtac
     6301 ggtggttttc acggaacgcg ctacccaaca cattcgcgtg ctcgacgtgg gaaccggtgt
     6361 ctctgacctg atcgatggcg gtcagttcgg cagcgtttcg atcgcgccgg atgccgcgca
     6421 cgtggcctat cagggcgtag accagatcat gaagttcgcc gaccgttcgg cgaacgtcac
     6481 gccggttctc cccggtggcg ggtgtgtggg gcccggcaca tggctcacga gccgctcgct
     6541 cgcctattgc gccaccatca atggcaactc ggggttcatg ttgctgccgg atctcgacgg
     6601 gaccgcaccg cggttcattg gtccgctacc tgccatctcg aatgatggcc atcagctcag
     6661 ctatgtggat gcgcgtggcg atgcggttat cgaggacatc gacggagcga atcgtcgcgt
     6721 ggtggtgcca tcgacggatc cgaccgcgct ctaccacttc cggggcgtca ccggcttcac
     6781 gcccgatgat cgcgcgatcc tgatctctga ctacgaaatg tatcccgcca agcttcgcat
     6841 cgtcgctctc gcagatggct cgagcatcga tgtggacgac gcgcttgatg gcagctctcc
     6901 attcgggcca acgttgttct acggcgcatc gcgcttttca ccagacggtt ccgagatcgt
     6961 cctgcagtca acgaccgggc tcgtcgcgat cgatctcggc accggcacga aacgcgtgct
     7021 cgcgccgttc gcgccacgaa cctcgagcgc gggcgcagtc tttctcgacg ccgagcacgt
     7081 actgtgggtg caggtcgaga atcatagtgt cagcgacatc ggcaagtaca cggcgtcgct
     7141 tcacgtcgca gggccgacgg tcgaggacga tcgcgttctc gatgcgccca acgacgagaa
     7201 cgcgctctat ccatccgttg ccgtgtcgcc ggagggtttc atcgcttggc ccggcaatgc
     7261 cttcatgatg acgatggccg gaaccgtcct ggtcgagaac gattactcgt cgccgagcgt
     7321 gtatgaggac atcctcggac tcacgccaaa cggacgcggc gtcatcgcga ttacctatga
     7381 cggcctcgtg cgctacgtcg acaggagtgg cgcgagtcga gatctcgcaa cggtgggcgg
     7441 caccggcgac ttgctcgcgc cgtacgccgc atatacgccc gcgtccacag cgtcccgccg
     7501 ccactaacgc gccgctttga tgagcccgcg ctccagaagc gcaccgccaa gcaaacagaa
     7561 gtacaggtag ccccggatcg ggccgaaccg ctcgacgata ggcgcgagcg acgaagcgat
     7621 ccggccgacg ttgcgggcga ccccaacgtc gccaggcgga aacacgtcga gacgcccgaa
     7681 cccacgcagc agcacgagcg cggcgctcca cgggccgatg cccggcaatg cggcgagaat
     7741 tgcgagtgcc tgctcggtgc tcgccgcggc aattgcctcg tcggtgagct tgccggatgc
     7801 gatcaagcgc gcgcagtttt tgagtgcgat cgctttgtgg ccgctcaggc cgcaacgacg
     7861 cagcgcttcg agccgcgcgc tggcgatcgt ggacgccgtc gggaatgcat aacgccgcgt
     7921 accagcgtgc tcgagcacga gcccgaaccg ctcgaccaat cgagtcacga tcgcgatgcc
     7981 tgcatcgagg ctcacttgct gaaacgggat cacgttcgcg aacgcctcga acagatcggc
     8041 gaagcgaggc ggtcgcattc cccgcagcgc acgtgcggtc gtgcgcagcg cgggcacggc
     8101 ttcggcgcgg cgctggagcg gacgaggatc gacatcgagg ccgagcttga cccggaggat
     8161 gcgctcgatt tcggttcggc tcgctttcga cgcgcttccg tgtgaaatcg agaagtgcac
     8221 gttcggagca tcgatcgtgc cgcggttttc gacctcgacg accgacaggc cggcgcgcgt
     8281 cgttatgacg cgaatgtagc ggttctgctc ccaacgctcg acgaggttcg tgggccgacg
     8341 ctgcaggaca cgcacggttg cttcgaggtg aaacggtgcg cgggtcgcga ggcgaagcat
     8401 ttcgaaggca tcctcacgac gtgcgatctg ctctgggttg cgtagttggg cgcgcactcg
     8461 tcacctgcca cggagcagga ctcaacaccg cgttcaggta gtcggctgca tcgtgagcgg
     8521 acggaccgaa cgaacggaat ccgatgtagc cgtcggggcg aacgatgtag atcgcggcct
     8581 gcgagacgcc gaaccgttgg aacacctcct ctgcgcccgc agtcgcctgc acgcgatgga
     8641 cgacgatggt tccggcgtgt cgatgctcga catcacgaag ctcgccctct ttgcacgggc
     8701 cgaaggcgag cacgtgaacg gcggtacctc gcaacagatc atgaagtgaa atgccatcga
     8761 gatcgagatc gggaacccgg cagcctgcct gaagcgcgct acgaaacgtc ggatcggcat
     8821 cgtcgctgac ttcgcgaacc gcgtagctcg ggtgataccg gatgtcgagc tgcgacgcga
     8881 agcgcacaac gcgcgagcgc agccgatacg atcgggcgac agcctggagc acgatcggcg
     8941 caatcagctc acgcagcacg cctgcgccga agcgatgcga cgtcgcgaga ccgaacgcac
     9001 gatcggtcgt gcgcacgagc acctcgccga cgcgctcccg ctcgagctcg taggtatcca
     9061 gcaactcgcg gctggcgccg cgcagcacga acgcgagctt ccacgccagg tttgtcgcat
     9121 cgccaaaccc ggtattcatc ccctggcccc caaacggcgt gtggatatga caggcatcgc
     9181 cggccaagaa tgcgcgtccg acgcaataac gccgcgtcgc ccggtgatga aggcgaaagc
     9241 gcgacgtcca cagtgcactg tcgatctgga tcggggcacg cgcggcgcgg gtgagtgcgt
     9301 tgatatcgtc gatcgtcacc ggcgcatcgg cggctggtga cggttggtcg acgaccacgc
     9361 ccatgatgcg cgtgcggtcg ccgaggacac cgacgaggca gacggcgtga cgcgtaatgt
     9421 agaccgcgaa cggcacgtca cgtggaagct ccacatccgc gagcatgaac tcggcgggat
     9481 acgtcgagcc ctcgaacgga atctcgagga cctcgcgaac gcggctgtgc gcgccatcgc
     9541 agccgacgag gtatcggcag cggaccgttt cgcgcgcggc cgaaccaatg tgcgcgagct
     9601 ccgcggtcac gccatcggga tcttggacga acgacacgag tcgcgacgaa cgttcgaccg
     9661 cgatgccgcg tgccgaaagg tcgtcgagca ggatccgctc ggtctcgggc tgcgcgagca
     9721 gatagagccc cgggtatcgc gtgatgccga ccagcgggtc gaagtgcagc tcggcaatcc
     9781 gcctaccgcg gacataggcg cgaatcggct cggcgcgtag tgcccgcgac gcgaattcct
     9841 cgccgagccc gagctgatcg aaaatttcga gagagcgcgc ctgaaggcca aacgcgcgcg
     9901 actcctgcgc gcgatcggcc tgctcatcga cgatacgaat tccgaccccg tatcgcgcga
     9961 gctgggtcgc catcatcagg ccggtagggc caccaccgac cacgagaacg tcggtcgttg
    10021 tctccatacc aacgcgcgtt gcatcggatg tgcccagcac gccgctgggc caccatccga
    10081 aatcaacgca ggacgacttc gcggtgacgc gcgcgctagc gacggcgccg gcggcggacg
    10141 atcagcgcga gcaccgagag tccgagcaca ccgctgccgg ccggcgagcg ctgcgactgg
    10201 cagcagccac cgtcgctgtc accggtctcg ggattgtcgg tcccaccatc gggcatgtca
    10261 ccgccatccg gccccggtcc cgcgcactcg ccggcgtcgg gaacgagcgt cgggaacggc
    10321 gtaccgacga tgccggtgaa cgcgacgtca ttgatctccc agcccgctcc accggtgcca
    10381 ccgtcggtca cgacgcggaa gcggagctgg aacgtcttac cagcgagcat cgtgccgaga
    10441 tcgagcgtga cggaatcggg attcggatac gacgcgttct tgttgccgaa cgcggcacgg
    10501 ccgccgagcg ggttgtccga cgtcgtcgtc agcgtgctgt tgtacggcga cgcggtcgcg
    10561 agagtggcga cgtcctgcca cgtcgcgccg ttgtcggtag agatctcgat cacgccaccg
    10621 tcccagtacg tgttgctgga gaactcgaac gagaacttgt ggctgaacgt gatcgagacg
    10681 ggcgccgtcg cgtcggcggt gagcgccggt gacgtcaggc tcgagtccgt gcgggcgccg
    10741 ctgtcgttgg cgaaccagat gccgtcgagc ggcgtcggcc gcgtatgcga ccactggctg
    10801 tccgtgctcg gcggcgtcca taccgatgcc atcgcgtcga acgagtcagt ggccgagctc
    10861 gcaaccttgt cgtcggtgtt gatggcgacg gcgagcggtt gctcgatcgt cgttgcacag
    10921 ccgtcgtcgg tggtcagcgt catggtgaag gcggcggcgg tcggatcgac gatcgagtcg
    10981 gcgaccgtga cgtcgaacga cgccatcgcg ctgccgtacg gcgcgatcgt gccgatcgac
    11041 accgggcccg atgcgacggt gacgccatcc gtggtcgtcg tcagggtcgc gacgacgttc
    11101 gtgagcgccg tcgggccggt gttcgcgatc ggcagcgtga ccttcgcggt ctcaccggcg
    11161 tcgagcacct cgtcggtatc acaactggtc gcgagcgcca tttgcgcagc gccgagcacc
    11221 gcgttgccct tgaccacgtt gctctcgacg atgcccatga aattcgagct cgagcgcggc
    11281 gccgataccg cacagcttcc gaagccgcga cgtgcatacg cggtcacgag cgtgtcatga
    11341 tcggccatgc ttgccgcgcg cgcagcggtg aggatcgcgt cgcgcgtttc cgtcggcgtg
    11401 gcgtcgggcg gcgcgagcaa gaggcccgcg acgacatact ggcgcatctt caggcgcacg
    11461 tcatcgaacg acgtgccggc tccctggagc gcgacgtagc cttcccacag catcgcggcc
    11521 cagacctcgc cggcgttgtg aacctcggcg ttgttgccgg aggcattgaa cggatgcgtc
    11581 gtcggcgtcg cgacgccatc cgtcatgtgg cggaacgaca gatcgttgat cgtgtggtcg
    11641 gcgctgtacg gcgcgcggcg gatgccaaag tacgccggat ccgccgagaa gctctgcgtc
    11701 gagtagatgc ccatcggata ggcacccatg aggttgtcac cgtcgcgcgc gacgacgagc
    11761 agcgagctga aatcgcccca gccttcgctc atcgcgccgc aggccgacgt tccgcacacg
    11821 ctcaaacggt gatgcacgta gtgaccgaat tcgtgcgcga tcacgctcgt gtcgagcgtg
    11881 ccctcgagct cgacgccgac ctcgcgctgc atcgtcacgt tgaccgcgcc cgccgcgata
    11941 tcggtcttga gctgcgtgcc gtcggtctgc aggatcgaca gcgtcgggat cgtgatcgcg
    12001 gtcgtgatcg tcgcgtcgtc ggcgagaccc ggaggcgacg tgctcgccgc gttgttcgcg
    12061 aggatcacgc cgatgccgcc ggcggtttgc acgttcaacg tcttcgtctt gaacgtgcac
    12121 gtgccgcggt cgacgagcac gatcttgccg gtgagcgacg gcagcgccgt gcacgcgtcc
    12181 gtcccggtgc cgacgccatc gtccgcgacc acgacctcgc cggtcacgtc gaagttcttg
    12241 ggcccgaacg acgcggagcc cgacgtcgtg gtgtgcgtgg cgatcgtcag cgtacgcgtc
    12301 tccttgccgc cccacaaaaa cacctgcatg cgcggctgca agccgtcgtc gggcgtggac
    12361 atgttcgcgt tgttgcgcga accaccgttt gcgttgtcct gcgcctcggc cttgatcgcg
    12421 tcacgatctt cgccgccgcg gccgtagttg ttgtcctgcg cgttgccggc agactcggtg
    12481 aatccgccgt cgtaccagaa gtcgtgcatc cagttgaccg tgtagaacag cgacgtgatg
    12541 ccggtcatct gctgggtttg ggtcgacagc ggctcggcat tgaagtcgta cgcgcgatcg
    12601 aacttgccag cggccgtcgt cgtggcgcgg aagtcgccga acgtgaggcc gtcgggcgcg
    12661 ttgaagtcgg cgtaggcctc gacgttgttg ccgagggtct ccgtgcggtt gttcacgagc
    12721 cacggatcgg caggccccgt gccgggatgg ttgaggccat cgaccgtcac gaggttgctc
    12781 gtcgcgtatg ccgggaacgc gccgttcgga tgtcccgttg catgcggggt gctgtcgacg
    12841 atcggaccgt cgaatggatg catctcaccc gtcgactcgg cgaacacgcg gtacgagaac
    12901 gccgcgtctg cgaccaggtt gcgttccgcg agcacgcggc cgtcctctgc gatcacgaga
    12961 cgaaccgcat cactcgaggt c
//
LOCUS       DASUFX010000245         8899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_871671,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000245 DASUFX010000000
VERSION     DASUFX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871671"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFV99_09610"
     CDS             <1..818
                     /locus_tag="VFV99_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059605.1"
                     /translation="ADALGLPKLTLRRELAFPDHGVLAYHGNVGDPINASPDGDATIG
                     DAIGSELILKFPRKLRAMVGGDHPGVEQIDDIDDVRPVYAVPAWVRQQSAIRRDLLKP
                     ISEVWSEVVDEFLANDFVKTWLKTQHKMFSLDLGKKLRLLLELSRNKVMAHGSDERLT
                     QLYKFFQHSFDGKMSQLAAMELGRRRNLRYVVNGHSHFPSMQPLGRIGDHPAVYFNTG
                     TWRAVHQIGHDIGGRPSFLPYDAMSYLVFFPDDDKLGREYEWWTGAMVTRGAH"
     gene            complement(915..1376)
                     /locus_tag="VFV99_09615"
     CDS             complement(915..1376)
                     /locus_tag="VFV99_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059606.1"
                     /translation="MKNITLAIAIALTLVSVGCKKKGGGAGEAMAKMTEFKDKMCKCA
                     EHDTKCAQAVSDEMTKWSQEQAKNQKEPPKMNEEDTKKAAAIGEEMGKCMQKAMTPDM
                     GAAPPAPAGGEAGSAAPAGGEAAPAAGGSAAPAAPAGGEAKPEEKKEEPKK"
     gene            1551..3188
                     /gene="gpmI"
                     /locus_tag="VFV99_09620"
     CDS             1551..3188
                     /gene="gpmI"
                     /locus_tag="VFV99_09620"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5059607.1"
                     /translation="MQLRRNPTFAGAKGPVVLVVMDGIGCGAGDEADAVKIAATPTLD
                     RLSAPGVRCELRAHGKAVGLPSDDDMGNSEVGHNALGAGKIYAQGALLVGRAIKSGAL
                     FEGATWKEVIARGARGGAIHFIGLLSDGNVHSHLDHLEAMLAAAAKTGCKKLYVHALL
                     DGRDVPPTSALEYVDRTERFLAGLRQSGVDARIVSGGGRMKITMDRYEADWEMVERGW
                     HTHVLADARVFKSAEEAIKTLRDEHPGVIDQDLPPFVVAPPTPMQDGDACVMFNFRGD
                     RAIELSRAFDDDKLDKFDRVRKPDVFYAGMMEYDGDLHVPKHYLVSPPEIEHPMGEIL
                     AEAHVPQLAISETQKYGHVTYFWNGNRSGMFDNGTETYIEIPSDRVSFDQRPWMKAAE
                     ITDRLITELRTGKHRFARVNYANGDMVGHTGSFDATVVAVETVDLQLARLVKAVEDVE
                     GILVVTADHGNADEMFQRDKHGRVQRDKQGQPIAKTSHTLNPVPFLIYDPMRRDRYEI
                     DAQRAQTAGIANVTATCLELLGFEAPDDLESSLLRFR"
     gene            3185..3697
                     /locus_tag="VFV99_09625"
     CDS             3185..3697
                     /locus_tag="VFV99_09625"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5059608.1"
                     /translation="MKTVFALVLVLAVASLAGCSQNEAKAACAGDECLPDLKYIDTTG
                     AAHTNDAMRGKVVVINFWATWCGPCKHEIPDLSKLYKKYKEKGVVMLGVLTNDNPTDA
                     ELLNFQSDFEMEFPVVRQNSDILVSFNYPQALPTTFIYDRAGRRVGKPRVGAIKPDEL
                     DATLAQLTTP"
     gene            complement(3694..4248)
                     /locus_tag="VFV99_09630"
     CDS             complement(3694..4248)
                     /locus_tag="VFV99_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059609.1"
                     /translation="MRRVLVALMMVFVCATAHAGPKKKQTAQLLSGVGASVSGAVVIT
                     GFMTAPDGKRFNAPVMYTGLSMLAITPSFGEFYAGQYLTWGMGIRAVAGALALYTLQT
                     QTHLAVCDNAHSSQDPPCEVFTEGAYPLLGLAAIGFIGGVWYDVLDSGDAVDRYNRAN
                     GFSVAPIVPTTSGLAPGMSLSGTF"
     gene            4451..5317
                     /locus_tag="VFV99_09635"
     CDS             4451..5317
                     /locus_tag="VFV99_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5059610.1"
                     /translation="MTLEVLFVLAMTSLGAATVNGALGYGYSSISVPIALLVVAGRVL
                     NPALVIVEVVINLYALAINWRSVPRVIKRVIPLAIGLVPGVIGGALLLGSVAPSHVKL
                     VAYGVLLPLILLQASGKRWPIRNERRASVPLGGGVGLLYGLTTISGPPLALFWNNQGL
                     EKNDFKTALAVVRSIESICALLAYAYLGLLTRDSASILPWIAPGVLIGFPLGHWLVRR
                     VGIETFRRVCMSFDAYLVSFGFARVLTAAGIAPALAYGVMVVTAIIDTKLLLAYFRPV
                     AEKTVDPATAVV"
     gene            5329..5745
                     /locus_tag="VFV99_09640"
     CDS             5329..5745
                     /locus_tag="VFV99_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059611.1"
                     /translation="MKTWWLAAVLLVGCASNDKPDIDATGTVTGQMTWGGGNCQKSGS
                     EPFTIFIAKIDSFNYNITTNTVGASIGGSVVCGPEYCQIMYFKQWSGANQESLSLDGT
                     LTLDGDTNAITGNGKYDSFDAVGSCEQQVTYTATLQ"
     gene            complement(5746..6771)
                     /locus_tag="VFV99_09645"
     CDS             complement(5746..6771)
                     /locus_tag="VFV99_09645"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX5059612.1"
                     /translation="MLERLEDKEPPRVRLANTPTRGHWLRYGEQLGARIWIKRDDHTG
                     SELSGNKVRKLEYLMAEALQQQATHVITCGGEQSNHARATAMAAAQLGLKSVLILRTE
                     TPDQPPSATGNILLDRLVGAEIRWISRPAWRDRNRLLAEEAERIKSAGGRPYIIPEGG
                     SNALGSWGYLKATRELVEDLAGIAAPDNPVTIVYPCGSGGTGAGLVLGWKLLGLSALG
                     IKVCGIAVCDDRAYFVDAIGKICGDFEERWQLDARVTPGDIDILDGHVGLGYAKSRPE
                     ELATIRDVCRSDGIVLDPVYTGKAFHGVVTELKKDPKRFGAAVAFVHTGGMFGLFANP
                     EQISSVL"
     gene            6830..7627
                     /locus_tag="VFV99_09650"
     CDS             6830..7627
                     /locus_tag="VFV99_09650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5059613.1"
                     /translation="MRARFVAACLVAFAVRADAKGWPSPAAGPTMTGDPELIFTFDDG
                     PNKDLTPLVLDILAKHHIHAVFFLVGEMAAQPTSPAIIERMLSEGHIIANHTMTHQDL
                     CRVKDPERAAHEIDDGKTAIETASHWHVTWFRTPYGVRCDRVDQLLAERGLTHFHWDL
                     DPQEWKHGNKKKAYDYVTKQVAKMTGRNVLLMHDIKKATVEALPEILDFIEAENVRRK
                     ETRRRRIRIIQGYELAAEKLPPGTLEWISAVTPSKQGVIDAIASVLP"
     gene            7658..8686
                     /locus_tag="VFV99_09655"
     CDS             7658..8686
                     /locus_tag="VFV99_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX5059614.1"
                     /translation="MSGPFRIRQSYREDVEQVLAVAEHLDTVNLPHDRGVIEGILDRS
                     ARSFAGELTGSDLEFVFVLEDIADKRIIGTSMIYAQHGTKRAPHIFFRVENDERYSVT
                     LDRYFVHRTLRIGYNYNGPTEIGGLILLPEYRRSTQALGKALSYVRFLFMRMHRPVFR
                     NQVLSELLPPLEPDGTSKLWEALGRRFTGLTYQEADRLSKDNKEFINALFPDDPIHTE
                     LLPDDVQAIIGQVGDETRAVEKMLRRIGFEYAEQIDPFDGGPHFIASTDDISIVRDAR
                     EAEVRGGGDGGERKWAIIAAETPGARPQFRAIGERVTIIDGGIALPEEARARLGVEVG
                     QKVWLSFG"
BASE COUNT         1590 a         2919 c         2806 g         1584 t
ORIGIN      
        1 tcgcggatgc gctaggcctg ccaaagctca cactgcgtcg cgagctcgcg ttccccgacc
       61 acggcgtgct cgcgtatcac ggcaacgtcg gcgatccgat caacgcgagc cccgacggcg
      121 acgcgacgat cggcgatgcg atcggctccg agctcatcct caagttccct cgcaagttgc
      181 gcgcgatggt cggcggcgat catcccggtg tcgagcagat cgacgacatc gacgacgtcc
      241 gcccggtcta cgcggtgccc gcgtgggtgc gccagcagag cgcgatccgc cgcgatctgc
      301 tgaaaccgat cagcgaggtg tggagcgagg tcgtcgacga attcctcgcg aacgacttcg
      361 tcaagacgtg gctcaagacg cagcacaaga tgttttcgct cgacctcggc aagaagctgc
      421 gcctcttact cgagctgtcg cgcaacaagg tgatggccca cggctccgac gaacgcctca
      481 cgcagctgta caaattcttc cagcacagct tcgacggcaa gatgtctcag ctcgcggcga
      541 tggagctcgg tcgccgccgc aacctgcgct acgtcgtgaa cggccactcg cactttccat
      601 cgatgcagcc gctcggccgt attggcgatc atccagcggt ctacttcaac accggcacgt
      661 ggcgcgcagt gcaccagatc ggacacgaca tcggcgggcg gccgagcttc ttgccgtacg
      721 acgcgatgag ctacctcgtg ttcttccccg acgacgacaa gctcggccgc gagtacgagt
      781 ggtggacggg cgcgatggtc acgcgcggcg cgcactgagg ctgcgcgtgc gccgaaaagc
      841 gaaacgccac gcaagctgcg tggcgttccg taatccaggc tgatctagct taagcagctc
      901 agctcggacg ccgattactt cttcggctct tccttcttct cttcgggctt cgcctcgccg
      961 ccagccggag ctgccggagc cgcgctgccg cctgccgccg gagccgcctc gccgcccgcc
     1021 ggggccgcgc tgccagcttc gccaccagcc ggagccgggg gagccgcgcc catgtcaggc
     1081 gtcatcgcct tctgcatgca cttgcccatc tcttcgccga tcgcggccgc cttcttcgtg
     1141 tcctcctcgt tcatcttcgg aggctctttc tgattcttgg cctgctcctg gctccacttc
     1201 gtcatctcgt ccgagacggc ctgcgcgcac ttggtgtcgt gctcggcgca cttgcacatc
     1261 ttgtccttga actcggtcat cttggccatc gcctcaccgg cgccgccacc cttcttcttg
     1321 cagccgacgc tgaccagggt cagcgcgatc gcgatcgcga gcgtaatgtt cttcatcaga
     1381 ttctcctttg cgaagcagtg tccgcctcgc gacgtggccc ggtcaacgaa gcggcggcgg
     1441 acgtccatcc atgatcgttt gcattgtcat cactcgatga cacgaatacg gcaatcgatg
     1501 acaggcctcg ccgcaatgtc gcgatcgcgg aacattgcta aggtccgcgg atgcagctgc
     1561 gccgcaatcc aaccttcgcg ggagcgaagg gtccggtcgt tcttgtcgtc atggatggca
     1621 tcggttgcgg tgcaggtgac gaagcagacg cggtgaagat cgccgcgacg ccgacgctcg
     1681 atcgcctatc ggcaccaggt gttcgttgcg agctacgcgc gcacggcaaa gcggtcgggt
     1741 tgccgagcga cgacgacatg ggcaacagcg aggtcggcca caacgcgctc ggcgctggca
     1801 agatctacgc gcaaggcgcg ctgctcgtcg ggcgcgcgat caagagcggc gcattgttcg
     1861 aaggtgcgac gtggaaagaa gtgatcgcac gcggtgctcg cggcggcgcg atccacttca
     1921 tcggcctgct ctccgatggc aacgtgcact cgcacctcga tcacctcgag gcgatgctcg
     1981 ccgccgcagc caagacgggc tgcaagaagc tgtacgtgca cgcgctcctc gacgggcgcg
     2041 acgtgccacc gacgagcgcc ctggaatatg tcgaccgcac cgagcggttc ctcgctggac
     2101 tgcgccaatc aggcgtcgac gcccgcatcg tgtccggtgg cgggcgcatg aagatcacga
     2161 tggatcgcta cgaagcagat tgggagatgg tcgagcgcgg ctggcacacg cacgtgctcg
     2221 ccgacgcgcg cgtcttcaag tctgccgagg aagcgatcaa gacgctgcgc gacgagcacc
     2281 ccggtgtcat cgatcaagat ctgccgccgt tcgtggtcgc accgccaacg ccgatgcagg
     2341 acggcgacgc atgcgtgatg ttcaacttcc gcggcgatcg cgcgatcgag ctctcgcgcg
     2401 cattcgacga cgacaagctc gacaagttcg atcgcgtgcg caaacccgac gtcttctacg
     2461 ccggcatgat ggagtacgac ggcgatctgc acgtgccgaa gcactacctg gtgtcgccgc
     2521 cggagatcga acacccgatg ggcgagattc tcgccgaggc gcacgtcccg cagctcgcga
     2581 tcagcgagac gcagaagtac ggccacgtga cctacttctg gaacggcaac cgcagcggca
     2641 tgttcgacaa cggcaccgag acctatatcg agatcccatc cgatcgcgtc tcgttcgatc
     2701 agcggccgtg gatgaaggcc gccgagatca ccgatcgcct catcaccgag ctgcgcaccg
     2761 gcaagcatcg cttcgcgcgc gtgaactatg cgaacggcga catggttggc cacacgggct
     2821 cgttcgatgc gacggtcgtc gcggtcgaga ccgtcgacct ccagctcgcg cgcctggtga
     2881 aggcggtcga ggatgtcgaa ggaatcctcg tggttaccgc cgaccacggc aacgccgacg
     2941 agatgttcca gcgcgacaag cacggccggg tccagcgcga caagcagggg cagccgatcg
     3001 cgaagaccag ccatacgctc aaccccgtgc cattcttgat ctacgacccg atgcgccgtg
     3061 accgctacga aatcgacgca cagcgcgcgc aaaccgccgg gatcgcaaac gtcacagcga
     3121 cctgtctgga gctactcggc ttcgaggctc cggatgatct agagtcgtcc ctactgaggt
     3181 ttcgatgaag actgttttcg cgctcgtgct cgttctcgcg gtcgcttcgc tggcggggtg
     3241 ttcgcaaaac gaggcgaagg cggcttgtgc aggcgacgag tgtctgcctg acctcaagta
     3301 catcgacacg accggtgctg cacacacgaa cgacgcgatg cgcggcaaag ttgtcgtcat
     3361 caacttctgg gcgacgtggt gtggcccgtg caagcacgag atcccggatc tgtcgaagct
     3421 gtacaagaag tacaaagaaa agggcgtcgt gatgctcggc gtcctgacga acgacaaccc
     3481 gaccgacgcc gagctgctca acttccagtc cgacttcgag atggagtttc cggtcgttcg
     3541 ccagaactcc gacatcctcg tctcgttcaa ttatccgcag gcgctaccga cgacgttcat
     3601 ctatgaccgc gctggtcgcc gcgtgggcaa accacgcgtt ggcgcgatca agcccgatga
     3661 gctcgacgcg acgctcgcgc agctgacgac gccctagaac gttccgctca gcgacatgcc
     3721 gggtgcgagg ccgctcgtcg tcggcacgat cggcgcaacc gaaaatccgt tcgcacggtt
     3781 gtagcgatcg accgcgtcgc cggaatcgag cacgtcgtac cagacgccgc cgatgaatcc
     3841 gatcgcggcg aggccgagca gcggatacgc accttctgtg aagacttcgc acggcgggtc
     3901 ttgcgagcta tgcgcgttgt cgcagaccgc caggtgggtt tgcgtctgca gcgtgtacag
     3961 ggcgagcgcg ccggcgaccg cgcgaatgcc catgccccac gtcaggtact ggcccgcgta
     4021 aaactcgccg aacgacggcg tgatcgcgag catgctgagg cccgtgtaca tgaccggcgc
     4081 gttgaagcgc ttgccatccg gcgccgtcat gaaacccgtg atcaccacgg cgcccgagac
     4141 gctcgcacca acgccggaca ggagctgagc ggtctgtttc ttcttcggac ccgcatgcgc
     4201 cgtcgcgcac acgaagacca tcatcagcgc gacaagcacc ctgcgcatgg ccggataaca
     4261 ccagaagtcg ggcgaggttg cccatgttcc gttatacttg acaacgtatc cgttataaca
     4321 gactatgccg ctatgatgat cgcggtgcac gacggcgagg tcgtcaaccc accatccgtt
     4381 atcgttgaca ggttgtccgc tcccggagta gcaatatatc cgttatggcg gacgaccgag
     4441 gagacctctc atgacactcg aagtcttgtt cgtcctcgca atgacgagcc tcggcgccgc
     4501 caccgtcaac ggcgcgctcg gttacggcta ctcgtcgatc tcggttccga tcgcgctgct
     4561 cgtcgttgcg ggccgcgtgc tcaatccggc cctcgtcatc gtcgaggtcg tcatcaacct
     4621 ctatgcgctc gcgatcaact ggcgctcggt gccgcgcgtg atcaagcgcg tcatcccgct
     4681 ggcgatcggt ctcgttcctg gcgtcatcgg cggcgcactg ctcctgggct cggtcgcgcc
     4741 aagtcacgtc aagctcgtgg cgtatggtgt gctgttgccg ctgatcctgc tgcaggcatc
     4801 aggtaagcgc tggccgattc gcaacgagcg ccgtgcgtcg gtgccactcg gcggcggcgt
     4861 cggcctgctc tacggtctca cgacgatctc cggtccgccg ctcgcgctgt tctggaacaa
     4921 ccaagggctc gagaagaatg acttcaagac cgcgctcgcc gtggtccgtt cgatcgaatc
     4981 gatctgcgcc ctcctcgcgt acgcgtatct cggcctgctc acgcgcgata gcgcatcgat
     5041 cctgccgtgg atcgcgcccg gcgtgctgat cggcttcccc ctcggtcact ggctggtccg
     5101 cagggtcggc atcgagacgt tccgccgcgt gtgcatgagc ttcgatgcgt acctggtgtc
     5161 gttcggcttt gcgcgggtgc tgaccgcggc cggcatcgcg cccgccctcg cgtacggcgt
     5221 gatggtcgtt acagcgatca tcgacaccaa gttgcttctc gcgtacttcc ggcccgtcgc
     5281 cgagaaaacc gttgatccag cgacggcggt ggtctaattg cagccaccat gaagacatgg
     5341 tggctcgctg cagttctgct cgtcggatgc gcttcgaacg acaagcctga catcgacgcg
     5401 accggcacgg tgacgggaca gatgacgtgg ggtggcggta actgccagaa gagcggcagc
     5461 gagccgttca cgatcttcat cgcgaagatc gactcgttca actacaacat cacgacgaac
     5521 accgtcggcg cttcgatcgg tggctccgtc gtgtgcggtc ccgagtactg ccagatcatg
     5581 tacttcaagc agtggagcgg cgcgaatcag gagtcgctca gcctcgacgg cacgctgacg
     5641 ctcgatggcg acacgaacgc gatcaccggc aacggcaagt acgactcgtt cgacgcggtt
     5701 ggcagctgcg agcagcaggt cacgtacacc gcgacgctgc agtgatcaca gcaccgagct
     5761 gatctgctcg ggattcgcga acaggccgaa catgccgccg gtgtgcacga acgcgaccgc
     5821 ggcaccgaat cgcttcgggt ctttcttcag ctcggtgacg acgccatgga acgctttgcc
     5881 cgtgtacacc gggtcgagca cgatgccatc gctgcggcag acgtcgcgaa tcgtcgcgag
     5941 ctcctcgggc cggctcttcg cgtagccgag gccgacgtgg ccatcgagga tatcgatgtc
     6001 acccggcgtg acgcgcgcat cgagctgcca gcgctcctcg aaatcgccac agatcttgcc
     6061 gatcgcgtcg acgaagtacg cccggtcgtc gcacaccgcg atgccgcaca ccttgatgcc
     6121 gagcgccgac aggccgagca gcttccagcc gaggacgagg cctgcgccgg taccgcctga
     6181 accgcacggg tagacgatcg tgaccgggtt gtcgggcgcg gcgatgccgg cgagatcctc
     6241 gacgagctcg cgcgtcgcct tcaagtagcc ccagctgccg agcgcgttgc tgccgccttc
     6301 cggaatgatg tacgggcggc caccggcact cttgatgcgc tcggcttcct cggcgagcag
     6361 gcggttgcga tcgcgccagg ccggccgcga gatccagcga atctcggccc cgacgaggcg
     6421 atcgagcaga atgttgccgg tcgcgctggg cggctgatcg ggagtttcgg tacgcagaat
     6481 cagcacgctc ttgaggccga gctgggcggc cgccatcgcc gtcgcgcggg catgattcga
     6541 ctgctcgccg ccgcacgtga tcacgtgtgt cgcctgctgc tggagcgcct ccgccatcaa
     6601 gtattcgagc ttgcggacct tgttgcccga cagctcgctg cccgtgtggt catcccgctt
     6661 gatccagatc cgggccccga gctgctcgcc gtatcgcagc cagtggccgc gggtcggggt
     6721 attcgcgagc cgcacgcgcg gcggttcctt gtcttccagg cgttcgagca tgcacggacg
     6781 ctacctcgaa atgaatgaca gcgacgcgcc gcgtgttaga tcgaacaacg tgagggctcg
     6841 ttttgtcgcc gcgtgcctgg tcgcattcgc cgttcgcgca gatgcgaagg gctggccctc
     6901 gcccgcagcc ggcccgacga tgacgggcga tccggagttg atcttcacgt tcgacgacgg
     6961 tccgaacaaa gacctgacgc cgctcgtcct cgacattctc gcgaagcacc acatccacgc
     7021 cgtgttcttc ctcgtcggtg agatggccgc gcagccgacc tcgcccgcga tcatcgagcg
     7081 catgctgagc gagggccaca tcatcgcgaa ccacacgatg acgcatcagg atctgtgtcg
     7141 cgtgaaggat cccgagcgcg cggcacacga gatcgacgac ggtaagactg cgatcgagac
     7201 ggcgagccac tggcacgtga cctggtttcg tacgccgtat ggcgtgcgct gcgaccgcgt
     7261 cgatcagctg ctcgccgagc gcgggctcac tcacttccac tgggatctcg atccgcagga
     7321 gtggaagcac ggcaacaaga agaaagcgta cgactacgtg accaagcagg tcgcgaagat
     7381 gaccggccgc aacgtgctgc tcatgcacga catcaagaag gcgacggtcg aagcgctgcc
     7441 cgagatcctc gatttcatcg aggccgagaa cgtgcgtcgc aaggagaccc ggcgccggcg
     7501 gattcgcatc atccagggct acgagctggc ggccgagaag ttgccgccgg gcaccctcga
     7561 gtggatcagc gcggtgacgc cgagcaaaca gggcgttatc gacgcgattg ccagcgttct
     7621 gccgtagagc tggacgtcgt ggtagggtgc ccttcgcgtg tcgggcccgt ttcgaattcg
     7681 tcaatcctat cgagaggatg tcgagcaagt gctggcggtt gccgagcact tggacaccgt
     7741 caacctgccg catgatcgcg gggtcatcga gggcatcctc gatcggtcgg cgcgatcgtt
     7801 cgcgggggag ctgaccggct ccgacctcga gttcgtgttc gtgctcgaag acatcgccga
     7861 caagcgaatc atcggcacgt cgatgatcta tgcgcagcac ggtaccaagc gcgcaccgca
     7921 catcttcttc cgcgtcgaaa acgacgagcg ctacagcgtc acgctcgacc gctacttcgt
     7981 gcaccgcacg ctgcgcattg gctacaacta caacggcccg accgagatcg gcggcctgat
     8041 cctgctgccg gaataccggc gcagcacgca ggcactcggc aaggcgctgt cgtacgtgcg
     8101 tttcttgttc atgcgaatgc accgcccggt attccgcaat caggtgctgt ccgagctctt
     8161 gccgccgctc gaacccgacg gaacctcgaa gctgtgggaa gcgctcggtc gccgcttcac
     8221 cggcctcacg tatcaagagg cggatcggct gtcgaaggac aacaaggaat tcatcaacgc
     8281 gctgttcccc gacgatccga tccacaccga gctattgccc gatgacgtcc aagcgatcat
     8341 cggtcaggtt ggcgatgaga cgcgcgcagt cgagaagatg ctgcgccgga tcggcttcga
     8401 gtacgccgag cagatcgatc cgttcgatgg cggtccgcac ttcatcgcga gcacggatga
     8461 catctcgatc gtgcgcgacg cgcgcgaagc cgaggtgcgc ggtggcggtg acggcggcga
     8521 acgcaagtgg gcgatcatcg cggcggagac gccgggtgca cggccgcagt tccgcgcgat
     8581 cggagagcgc gtgacgatca tcgacggcgg catcgcgctg cccgaggaag cgcgtgcgcg
     8641 gctcggcgtc gaagtcggcc agaaggtatg gctgagcttc ggctgacgca ggcctcgggc
     8701 ctcaggcctc aggcctccgt ggggcttcgg gcttcgggct gcatcgtgct cgctcttggt
     8761 tttggtcttg gcgcgtgtcg atcttcggag gcaggatcag gatcgggtct caaacctcca
     8821 cagggctggc aaccgctacc cgagctcgcg acggcagctg ccgatgccgc aaaggccgga
     8881 catctgatcg tggatggtg
//
LOCUS       DASUFX010000246         7103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_37268,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000246 DASUFX010000000
VERSION     DASUFX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37268"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..350
                     /gene="rpsP"
                     /locus_tag="VFV99_09660"
     CDS             102..350
                     /gene="rpsP"
                     /locus_tag="VFV99_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5059615.1"
                     /translation="MAVALRMSRQGKKKSPFYRIVAADKRCARDGRFIELLGTYHPVT
                     KVLKLDHERYQKWIKVGAQPSGTLDAVIRRTARATKTA"
     gene            350..598
                     /locus_tag="VFV99_09665"
     CDS             350..598
                     /locus_tag="VFV99_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5059616.1"
                     /translation="MAREPDLAELIRYLAVNLVDKPDEVRVELVEERQANVYELEVAE
                     SDLGKIIGRGGKTARALRTVVQSIAPRSRKRTLVEILE"
     gene            686..1210
                     /gene="rimM"
                     /locus_tag="VFV99_09670"
     CDS             686..1210
                     /gene="rimM"
                     /locus_tag="VFV99_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5059617.1"
                     /translation="MRGEARIEIGGVARAHGIRGEVVIVTHDPDSATLGSVDTIWVGG
                     VARKVLQARDTQRGWLVALEGVVTRNDAEALKGQQVEVDREDLELDPDDVLLDDMIDC
                     EVRLPDGTPWGTIAEIMVGAHQDLLVIHDGEIERLLPLVDEFVTSIDLENGVVTVSPP
                     EDLPESKKKPSKKL"
     gene            1207..3084
                     /gene="trmD"
                     /locus_tag="VFV99_09675"
     CDS             1207..3084
                     /gene="trmD"
                     /locus_tag="VFV99_09675"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5059618.1"
                     /translation="MRFTVVTILPELIEPSLTAGVVGRAREAGVITVDTINPRDFTRD
                     KHRTVDDTPYGGGPGMVMKAEPLLAAIAKACEPRTPSTPAPLDTPEKLDLLGQPLDPL
                     TKVARAIAKVESMLGGLGGAAGPLTSAQLENDSTHVEDAEAALAEVEKELQKEALAQA
                     LTPPTDSDQIDTRPSAKVNEDIDLHLPARNSRLGALGPADADDGRVRTEAVPEDSHQE
                     MPHLVEKRNIVQTPPAGVPVVQPPAEATNDATSDALPQPAEATPTTDRTKPHRILLSP
                     SGTPLTQARVRELAKHDHLVLVCGRYEGVDQRVIDLAIDEELSIGDYVLSGGELGALV
                     IIDAVARYVPGVLGEPTSADDESFSAGLLEYPQYTRPQEITMPAALQSDRIASPVPPV
                     LQSGNHAAINAWRRQQAMQRTAARRPDLWRHVRTTKADDKLFAPLRARTHLALVHHPI
                     VDRTGAVVTTALTNFDIHDLARSSLAYGLAGYHIVTPIASQREKAEHIASLWMADEQG
                     EHRAAALRLIRTAESVETVIAALTAEHGAAPTVLATSARAESFPAAARRTSSELLAEA
                     SQNPAPLLILLGTGWGLADKLIPSVSRVLAPIEGASEWNHLSVRSAGAVILDRLFGRP
                     A"
     gene            3147..3491
                     /gene="rplS"
                     /locus_tag="VFV99_09680"
                     /pseudo
     CDS             3147..3491
                     /gene="rplS"
                     /locus_tag="VFV99_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(3719..4315)
                     /locus_tag="VFV99_09685"
     CDS             complement(3719..4315)
                     /locus_tag="VFV99_09685"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5059619.1"
                     /translation="MRSVLVEGYGRPYSDGDMLDHGSPLVFTVDDVLSAAECAEVVTR
                     IEALGPTAAPITTSRGFEMRPDVRNNTRVIFDDVTLAQTLFERVAPHLPLRLCNMTPA
                     GANERFRCYRYENEQRFAPHYDGAFQRNEQERSLLTFMVYLNEGFGGGATTFHDFDVS
                     VVPKTGRALLFQHFLLHEGCSVTSGVKYALRSDVMYRA"
     gene            complement(4364..4627)
                     /locus_tag="VFV99_09690"
     CDS             complement(4364..4627)
                     /locus_tag="VFV99_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059620.1"
                     /translation="MWTAIKWLLGGGSDAERAAWREFRKDHVVYIPRVDTVADEPTRV
                     IVRIRFTPIRQPTFYRFYAVDRVTLKVELIVDDSAYPPSNRLR"
     gene            complement(4705..5070)
                     /locus_tag="VFV99_09695"
     CDS             complement(4705..5070)
                     /locus_tag="VFV99_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5059621.1"
                     /translation="MQMFGDDKATIEVGATGEDRAVDHLMRNGLRIVERNYRCKVGEL
                     DVVARDGKTLVFVEVRSRRTEEYGSALDAVSWHKRRKVTRVALAYLAARKPRFEAARF
                     DVVAITGNELVHIKDAWRL"
     gene            complement(5156..5644)
                     /locus_tag="VFV99_09700"
     CDS             complement(5156..5644)
                     /locus_tag="VFV99_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059622.1"
                     /translation="MIRLAMVLGLAGLACLGACTAEPVGRICDLGTALPAPSEVVVAS
                     PSLDCVSRTCLRVPLGRELPMGSMFPEGNNGLCTAECDSSSDCERVPESPCKTGFTCG
                     VAVTVGPFCCKKFCICKDYIVIPDSGSLATPLACDSGDPANACCNLSGRTGNSQYPLC
                     KG"
     gene            5935..>7103
                     /locus_tag="VFV99_09705"
     CDS             5935..>7103
                     /locus_tag="VFV99_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059623.1"
                     /translation="MAALAVTLTVGTAFAQKKGAGKDMEFEPESAASTPPSKTLERAI
                     KLYDKKDFFSASIELKKVLDGESGDDAKNKQRAEFFMGKTLYQMGFYAGSLAYFDKIV
                     QAGDNHTYHAAALKWLAALSRVLPETSGILEKIGTYDPSALNDPSLASVKDELLFLLG
                     RHYYRRGGDGDFEKAISLFQQVDRQSGMFIKAKFFEGVTYVRKYEGKPAVDAFKEILI
                     IGEERPKQYSSDDVDNFRELAQLQMARVFYSTQQFDTSIKYFEKLPQDSADWAESLFE
                     ASWAYFMKTLNSKALGNIHTLNAPYFENQFYPEAVLLRAVIYFKYCLYDQAEEAIADF
                     NEKYTPLTKNLQDIVKKYDDNAEFFAYIQKVRRGQAGLDPVTQRLVISVLNDKTLL"
BASE COUNT         1334 a         2370 c         2254 g         1145 t
ORIGIN      
        1 gagtccccga gcagccacaa cgcggcgcat cccggctcat atagcacgtt gacaggccct
       61 aagtgcctgg gtagattgcc gcgctcgcaa aagaggatct catggctgtt gcgcttcgca
      121 tgtcccgtca gggaaagaaa aagtctccgt tctatcgcat cgtcgctgcg gacaagcgct
      181 gcgcccgtga tggtcggttc atcgagttgc tcggtacgta ccacccggtc accaaggtcc
      241 tgaagctcga ccacgagcgc tatcagaagt ggatcaaggt cggcgcccag ccgagcggca
      301 cgctcgacgc ggtgattcgt cgtacggccc gcgcaacgaa gactgcctaa tggcccgcga
      361 acccgatctc gcggagctca tccgctatct cgcggtcaat ctcgtcgaca aacccgacga
      421 ggtccgcgtc gagctcgtcg aagaacgtca agcgaacgtc tacgagctcg aagttgccga
      481 gtccgatctt ggcaagatca tcggccgtgg cggcaagacc gctcgcgcgc tgcgcaccgt
      541 cgtccagtcg atcgcgcccc gctcgcgcaa gcgcacgctg gtcgagatcc tcgaataaca
      601 gcccgcgctg cgcacgccgg cgatttccgc gcggcgatgc gctcgcacaa gccgcacgtc
      661 tcagcgacac tggggcgcaa tagccatgcg cggtgaagcg cggatcgaaa tcggcggggt
      721 agcgcgggcg cacggcatcc gcggcgaggt cgtgatcgtc acgcacgatc cggactccgc
      781 cacgctcggc tcggtcgaca cgatctgggt cggcggcgtc gcgcgcaagg tgctccaggc
      841 gcgcgatacg cagcgcggct ggctggtcgc gctcgaaggc gttgtcacgc gtaacgacgc
      901 cgaggcgctg aaaggccagc aggtcgaggt cgatcgcgag gatctcgagc tcgatcccga
      961 cgacgtcctg ctcgacgaca tgatcgactg cgaggtccgc ctccccgacg gcacgccgtg
     1021 gggcacgatc gccgagatca tggtcggcgc ccaccaagac ttgctcgtca ttcacgatgg
     1081 cgagatcgag cgtctgctgc cgctcgtcga cgagttcgtg acgagcatcg atctcgagaa
     1141 cggcgtcgtc accgtgagcc cgccggagga cttgccggag agcaagaaga agccgagcaa
     1201 gaagctatga ggttcacggt cgtcacgatc ctgcccgagc tgatcgagcc gtcgctcacg
     1261 gccggcgtcg ttggccgtgc gcgcgaggcg ggcgtgatca cggtcgacac gatcaacccg
     1321 cgcgacttca cccgcgacaa gcaccgcacc gtcgacgaca cgccgtacgg cggtggtccg
     1381 ggcatggtga tgaaggccga gccgctgctc gccgcgatcg cgaaagcgtg cgagccacgt
     1441 acgccaagca cacccgcgcc actcgacacg ccggagaagc tcgatctgct cggccagccg
     1501 ctcgatccac tgacgaaggt cgcgcgcgcg atcgcgaagg tcgagtcgat gctcggcggg
     1561 ctcggtggtg ccgcggggcc gctcacctcg gcgcagctcg agaacgactc gacgcatgtc
     1621 gaggatgccg aagccgcgct cgccgaagtc gaaaaggagc tgcagaagga agcgctcgcc
     1681 caggcgctga cgccgccgac cgatagcgat cagatcgaca cgcggccgag cgcgaaggtc
     1741 aacgaagata tcgatctgca cctgccagcc cgcaattcac ggctcggtgc actcggcccc
     1801 gccgacgccg atgatggtcg cgtgcgcacc gaagcggtgc ccgaggattc gcaccaggag
     1861 atgccgcatc tcgtcgagaa gcggaacatc gtgcagacgc cgccggctgg cgtgcctgtc
     1921 gtgcagccgc ccgccgaggc gacgaacgac gcgacgagtg acgcgttgcc gcaaccggca
     1981 gaagcgacgc cgacgaccga tcgcacgaag ccgcatcgca tcttgctgtc gccatcgggc
     2041 acgccgctca cgcaagcgcg cgtgcgcgag ctcgcgaaac acgaccatct cgtgctcgtg
     2101 tgcggtcgat acgaaggcgt cgaccagcgc gtgatcgatc tcgcgatcga cgaggagctc
     2161 tcgatcggcg actacgtgct ctccggcggc gagctcggcg cgctcgtgat catcgacgca
     2221 gtcgcgcgct acgtgccggg cgtgctcggt gagccgacgt ccgccgacga cgaatcgttc
     2281 tccgccgggc ttctcgagta tccgcagtac acgcgcccgc aagagatcac gatgcccgcc
     2341 gcgctgcaga gcgatcggat cgcgagcccg gtgccgccgg tgctgcagtc gggcaatcac
     2401 gcagcgatca acgcgtggcg ccgccagcaa gcgatgcaac gcacggcggc gcgccgtccc
     2461 gatctatggc gccacgtgcg cacgaccaaa gccgacgaca agctgttcgc gccgcttcgc
     2521 gcgcgcacgc acctcgcact cgtccatcac ccgatcgtcg atcgcaccgg cgccgtggtc
     2581 acgaccgcgc tgacgaactt cgacatccac gatctcgcgc gctcgtcgct cgcgtacggg
     2641 ctcgcgggct atcacatcgt cacgcccatc gcgtcgcagc gcgaaaaggc cgagcacatc
     2701 gcctcgctct ggatggccga cgagcaaggc gagcatcgcg ccgccgcgct ccgcctcatc
     2761 cgcaccgccg agtcggtcga gaccgtgatc gcggccctga cggccgagca cggagccgcc
     2821 cccacggtcc ttgccacctc ggcccgggcc gagtcgtttc cggccgccgc ccgccggacc
     2881 agctccgagc tactcgcaga ggcctcccaa aaccccgccc cactcctgat tctcctcggc
     2941 accggctggg gtctggcgga caagctgatt ccctcggtat cccgcgttct tgcgccgatc
     3001 gaaggagctt cggagtggaa tcatctctcc gtccgatccg ctggcgctgt gattctcgac
     3061 cgtctgttcg gtcgcccggc ttgacgccca ggggggttct cccgtagaac cctgcgtcca
     3121 atcggccatt tgccggggtc ttcagtatga gcgctcaacg aattctcgag tccatcgaca
     3181 agagccagct tcgccaggcg gccttgccag agttccgccc cggcgattcg atcaaagtgt
     3241 gggcgaagat tcgcgaaggc gagaagaccc gcctccaggc gttcgaaggc gtctgcatcc
     3301 gccgcgtctc gaagggcgcg cggtcgacgt tcaccgtccg caagatttcc tacggcgtcg
     3361 gcgtcgagag gatcttcccc gacaactcgc cgaacatcga caaggtcgaa gtgatcgcac
     3421 gcggtcgcgt tcaccagtcg cgcctctact acctgcgcga cctctcgggt aaggccgctc
     3481 gcatcaagga gcgcgaaggc acgggcgaag agatcgcgtc ggccgaggct gcggatgcct
     3541 ccgcgggccc cgaaggtggc gaggctgccg ctgaaggtgg cgaggctgcc gctgaaggtg
     3601 gcgaggccgg caaggccaag aagacgaaga aggcgaagaa ggcctgagca gcacgcgctg
     3661 ctagcagtcg aaatgcgcgc tccggcgcgc atgtttgttt tcggcgcgta gcgctgcttc
     3721 aggctcggta catcacgtcg ctgcgcagcg cgtacttcac cccgctcgtc acgctgcagc
     3781 cctcgtgcag gaggaagtgc tggaagagca gggcgcgtcc cgttttcggc acgacgctga
     3841 cgtcgaagtc gtggaacgtc gtcgcgccgc cgccgaagcc ctcgttcaga tacaccatga
     3901 acgtgagcag gctgcgctcc tgctcgttgc gctgaaacgc gccgtcgtaa tgcggcgcga
     3961 atcgctgctc gttctcgtag cgatagcagc gaaaccgctc gttcgcgccg gcgggcgtca
     4021 tgttgcacaa ccgcagcggc agatgaggcg cgacgcgctc gaacaacgtc tgcgcgagcg
     4081 tcacgtcgtc aaagatcacg cgcgtgttgt tgcgaacgtc ggggcgcatc tcgaaccctc
     4141 gtgacgtcgt gatcggcgcg gccgtcggtc cgagcgcctc gatgcgcgtg acgacttcgg
     4201 cgcattcggc ggcggacagc acgtcgtcga ccgtgaacac gagcggcgat ccatgatcga
     4261 gcatgtcgcc gtcgctgtac gggcgcccgt agccttcgac cagcacgctg cgcatcatga
     4321 cgattatggg cggcggtgtg gtccgccgga agtctttcga gcgtcatctc agacggttgc
     4381 ttggcggata cgcactatcg tcgacgatca gctcgacctt cagagtcacc cgatcgacgg
     4441 cgtagaaccg gtagaacgtc ggctgtcgta tcggtgtgaa gcgaatccgt acgatgaccc
     4501 gggtgggttc gtcggcaacc gtgtcgaccc gagggatgta gacgacatga tccttacgaa
     4561 actctcgcca ggcagcgcgc tccgcgtcgc tgccgcctcc caacagccat ttgatcgcgg
     4621 tccacatcga ggcagcctac ctccttcaac gatcagccgt ggacctgatt gcccggcaca
     4681 ggtcacgcga gcggccggat cgagctagag ccgccaggcg tctttgatgt ggacgagctc
     4741 gttgccggtg atcgcgacga cgtcgaaacg cgcggcctcg aagcgcggct tgcgcgcggc
     4801 gagatacgcc agcgcaacgc gtgtcacctt tcggcgcttg tgccagctca ccgcgtcgag
     4861 cgcgctgccg tactcttcgg tgcgccggct gcgcacctcg acgaacacga gcgtcttgcc
     4921 gtcgcgcgcg acgacatcga gctcgccgac cttgcaccgg tagttgcgct cgacgatccg
     4981 cagcccgttg cgcatgagat gatcgaccgc gcgatcctca cccgtggcgc cgacctcgat
     5041 cgtcgccttg tcatcaccaa acatctgcat cacaccggtc tagtcgacgg ctctgacgcc
     5101 gaaaacaaca aaggcagcct cgcggctgcc tccgtggttg tccgctacgc ggacgttagc
     5161 ccttgcagag cgggtactgg ctgttgccag tgcggccgga caggttgcag catgcgttcg
     5221 ccggatcgcc cgagtcgcag gccagcggcg tggcgagcga gcccgagtcc gggatcacga
     5281 tgtagtcctt gcagatgcag aacttcttgc agcagaacgg accgacggtc accgcgacgc
     5341 cgcaggtgaa gccggtcttg cacggcgact ccggcacgcg ctcgcagtca ctcgagctat
     5401 cgcactcggc ggtgcagagg ccgttattac cctccgggaa catcgagccc atcggcagct
     5461 cgcgacccag cgggacacgc aagcaggtgc gcgacacgca gtcgagcgac ggcgaagcta
     5521 cgacgacttc cgacggcgca ggcagcgccg taccgaggtc gcagatgcgt ccgactggtt
     5581 cagccgtgca tgcgccaagg catgccaggc ccgcgagccc caggaccatc gctagtcgaa
     5641 tcatgcgccc cgataataaa gagcctggta cggcagtcaa gcgaacgacc atgaacaacg
     5701 ccttgttgca gttggtagat ccgttttctt gcatggcgac tattggtgac gggtggaggg
     5761 ccgacccgtc actaacaccc gcatgaccca attgcgcaac agaagcgatc gatcgtgtgt
     5821 cgcgagcggg tcgttggctt gacacacatg ggtcagcaca ttgatgattt cgccgcctac
     5881 tccgtgatcg gagccgggca ccggaggacc ttgatgcgcc tgaaaatcct atcgatggcc
     5941 gcgctggccg tgacgttgac ggtcggaacc gccttcgccc agaagaaggg cgccggcaaa
     6001 gacatggaat tcgagcccga gagcgccgct tcgacgccgc cgtcgaagac gctcgaacga
     6061 gcaatcaagc tctacgacaa gaaggacttc ttctcggcgt cgatcgagct caagaaggtg
     6121 ctcgacggcg agagcggtga cgacgcgaag aacaagcagc gcgccgagtt cttcatgggc
     6181 aagacgctct accagatggg cttctacgcc ggctcgctgg cctacttcga caagatcgtc
     6241 caggccggcg acaaccacac gtatcacgca gcagcgctca agtggctcgc tgcactgagc
     6301 cgcgtgctgc cggagacgtc gggcatcctc gagaagatcg gtacctacga tccgagcgcg
     6361 ctcaatgatc cgtcgctcgc gagcgtgaag gacgagctgc tgttcctcct cggccgccac
     6421 tactaccgtc gtggcggcga cggtgacttc gaaaaggcca tcagcctgtt ccagcaagtc
     6481 gatcgtcaga gcggcatgtt catcaaggcg aagttcttcg agggcgtcac gtacgtccgc
     6541 aagtacgaag gcaagccggc cgtcgacgcg ttcaaggaga tcctcatcat cggtgaggag
     6601 cgcccgaagc agtacagctc ggacgacgtc gacaacttcc gcgagctcgc gcagctgcag
     6661 atggcgcgcg tgttctactc gacgcagcag ttcgacacgt cgatcaagta cttcgagaag
     6721 ctgccgcagg attcggccga ctgggcggag tcgctcttcg aggcgtcctg ggcgtacttc
     6781 atgaagacgc tcaactccaa ggcgctcggc aacatccaca cgctcaacgc gccgtacttc
     6841 gagaaccagt tctacccgga agcggtgctg ctccgcgccg tcatctactt caagtactgc
     6901 ctctacgatc aggccgagga agcgatcgcg gacttcaacg agaagtacac gccgctcacc
     6961 aagaacctgc aggacatcgt caagaagtac gacgacaacg ccgagttctt tgcgtacatc
     7021 caaaaggtgc gcagggggca ggccggtctc gatccggtca cgcagcgcct cgtgatcagc
     7081 gtgctcaacg acaagacgtt gct
//
LOCUS       DASUFX010000247         7068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1843245, whole genome shotgun sequence.
ACCESSION   DASUFX010000247 DASUFX010000000
VERSION     DASUFX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843245"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFV99_09710"
     CDS             complement(<1..810)
                     /locus_tag="VFV99_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX5059624.1"
                     /translation="MPNLLAMSFEGELAPSFTLHCLDVGRTLPDGWGMGYYPAGEPSA
                     AILKEPAPPQGSIRSQLALAWEQVASSLFVLHIRHATWGALSDANTQPFARAWGRRDW
                     LMAHAGSLDKKLPEIAGPFEPIGSTDTEQLFCQLLNKCVERNWRSLGEVDIGVMVSWI
                     SELNEHGGMTMCLTDGRDLLVHADRRGDPLWLGQLTPPYDKIAFGDDDLGIDLTRRGA
                     KARKGVVISTEPQKPRDGDADKALTWKQLAPGQLLLVREGAVIGEHSTGAQA"
     gene            908..1273
                     /locus_tag="VFV99_09715"
     CDS             908..1273
                     /locus_tag="VFV99_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059625.1"
                     /translation="MTKALVVLVLLSACDSPGRRAQDAAPIDVVGEPQPLPPDAAPPP
                     FDAAPLPEMACTDAPDAGMQCALPKSTCLDQYYLTYYTAGTCTNSKCTFEEHWLYCYA
                     GCSSNISGDGNAGCEGGFT"
     gene            1252..1659
                     /locus_tag="VFV99_09720"
     CDS             1252..1659
                     /locus_tag="VFV99_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059626.1"
                     /translation="MRGRIYLTRTHLVATIIVCVVLPLASWLDGSGQLAWTMFSRTGQ
                     FRLVIVADGTQMNPTEIAAAAMPGPTAIALAGSDQLKHHDVMRATARRHLDDIADLAC
                     RLRPGAQLTVRLEERVRRSEPIRTSEVTRRCAR"
     gene            1656..2495
                     /locus_tag="VFV99_09725"
     CDS             1656..2495
                     /locus_tag="VFV99_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5059627.1"
                     /translation="MKLASLRDPVGFVRDALVLVRDPLALSRGAFALVFLLRTTPLLA
                     PCDPRFLRGTNGWLGWPDGGWHIPVHGLALPAAVVMALVVLRTLGALALMLGIYPRVS
                     GLAAGIAGYLVLAQDAFGYMHHLHMLYLGAILFALVDRDAPRSSLLMLRLFVASIYVW
                     AVIGKLASEWGTGVPLTAFVGNGALDRDLVGTLLATPERCAIVEIVVMVIEAAIAAGL
                     LWPRTRKIALVCAVAMHIGFEIIGRVDSIGWQMMALLLVFTGDSEPPRTDTRSSTDHT
                     SRR"
     gene            complement(2428..3270)
                     /locus_tag="VFV99_09730"
     CDS             complement(2428..3270)
                     /locus_tag="VFV99_09730"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059628.1"
                     /translation="MRCAWLWLVAVGCRAAPDADVVLRFTPDPGAPGTERYQCFGFAA
                     TPLADADIGGITFAPGTGPVAVHHITLYASPSPFAAGPVDCLEMPDDAVPMNVWAPGG
                     GDLALPADVSLVIPGGTTHLIVQTHALRNDDGAASEDAITLAPREPALHRAGWLPLRA
                     PVPVIQPGQRTEVTATCTVDAALHLVSTWPHMHQIGSEFHGAIVRGSTASEALVDVVP
                     WVFDTQHAYPVDAQLAAGDAISTHCIWQNATSQPVLPGPRITDEMCGQSMIAYPYEAA
                     RCHQ"
     gene            complement(3318..4130)
                     /locus_tag="VFV99_09735"
     CDS             complement(3318..4130)
                     /locus_tag="VFV99_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059629.1"
                     /translation="MRRCWLLVLVAACEADDPTVSLRFTPDPGPPGSELYQCFAFDVS
                     LLGGSDIGAIRYIAAESAVTLHHVSVWASRTRFPAEPVECQEFPGDAVPMNVWVTGSG
                     DLALASNISLVIPEGTQQIVVQTHSLRVDEGPAVERVVEVVPRAPAEHRAGWFELRAN
                     VPVIEPFSRAEAYSQCALAESLHVISAWPHMHRMGTEFHGLVDVAQFRFDNQQTYPLD
                     LDFAAGAVIPTHCIWQNTSDRAISPGFGIEDEMCGQAAIVYPYTAARCSFRL"
     gene            complement(4151..5122)
                     /locus_tag="VFV99_09740"
     CDS             complement(4151..5122)
                     /locus_tag="VFV99_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX5059630.1"
                     /translation="MISRVADHCFWFGRYVERAESTARMLAATASLALDADLPAAQVW
                     RPVVVVSGEEDAFRARVADGNDDAAAWGDGEVVQRFMVWDEANGVSIARSIGGGRWNA
                     RSVREVLSLEAWETINELHLWFTSTAAHAVYANQREAFYQQVRRSTQLTLGLLRSTML
                     HDEPLDFIWLGVLLERVSQTARILDVHHHAFTNLPRRHEIVETGLWLSLLRALSGSEA
                     YMKRAAGRISSETVAKFLISEDAFPRSIAYCVHSAYERLCAIRPPEQHELPGGVALER
                     LRVLDTWVAAREKEPLTGNGVHELLTHVVDEVADIGHTLGKELLGAG"
     gene            complement(5119..6603)
                     /locus_tag="VFV99_09745"
     CDS             complement(5119..6603)
                     /locus_tag="VFV99_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX5059631.1"
                     /translation="MQQQSQSQGTAGGLFEGYPALPNTYDEMFEQGIARPPFARVAQL
                     LGKLGKDELARAQALAEHALLQQGVTFSVYGDSRGTEKIFPFCLVPRMIAGGDWARLE
                     RGLQQRLRALGMFLDDIYGEQKILEAGVVPAEMILGAAGYRPMLRGLKPPGGVRIHIS
                     GIDLIRDPQGTWRVLEDNLRTPSGVSYVLENRSLSKRMFPKVFDAARVHRVDHYPTRL
                     VDALRSVSPAPDGDTCAVVLTPGPYNSAYFEHTFLARTMGLELVQAPDLFVDQDKVFC
                     RTTRGPRRVDVIYRRTDETFLDPEVFRPDSVLGVPGLMRAYAAGNVALANAPGNGVAD
                     DKAVYPFVPQMIEFYLGEQPLLDQVQTYVCLRDDDRKYVLEHLGEMVVKAVDEAGGYG
                     MLMGPQSTAEQREEFRARIIAEPRRYIAQHRVELSSCPTWIGDRNVVEPRRVDLRPYI
                     VTSREGPWVLPGGLTRVALVAGSYVVNSSQGGGSKDTWVMKEST"
     gene            complement(6691..>7068)
                     /locus_tag="VFV99_09750"
     CDS             complement(6691..>7068)
                     /locus_tag="VFV99_09750"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX5059632.1"
                     /translation="IEGPGPDRVAIAGKWAHLRAIGDGARAIGVRRRIYQPSPGLHPG
                     LPVTDPLVIDWTWAGRAQHIELWAWRPGGGPYDGLPHDDTDALTRRQERILISTREGD
                     VTAASFWREARPFTIDLRRDGIA"
BASE COUNT         1122 a         2362 c         2413 g         1171 t
ORIGIN      
        1 ggcctgcgcg cccgtcgagt gctcgccgat cacggcaccc tcgcgcacga gcaggagctg
       61 gcccggcgcg agctgcttcc aggtcagcgc cttgtcggca tcgccgtcgc gcggcttctg
      121 tggctccgtc gagatcacga cgcccttgcg cgccttcgcg ccgcgtcgcg tgagatcgat
      181 cccgagatcg tcgtcaccga atgcaatctt gtcgtacggc ggcgtgagct gaccgagcca
      241 cagcggatca ccgcggcgat cggcgtgcac gagcaagtct ctgccgtcgg tcaggcacat
      301 cgtcatgccg ccgtgctcgt tgagctcgct gatccagctc accatcacgc cgatgtcgac
      361 ttcgccgagc gagcgccagt tgcgttcgac gcacttgttg agcagctggc agaacagctg
      421 ctccgtatcg gtcgatccga tcggctcgaa cggcccggcg atctcgggca gcttcttgtc
      481 gaggctgccg gcgtgcgcca tcagccagtc gcggcggccc cacgcgcgcg cgaacggttg
      541 cgtgttcgcg tcggagagcg cgccccacgt ggcgtggcga atgtggagca cgaacaacga
      601 cgacgcgacc tgctcccacg cgagtgcgag ctggctgcgg atcgaaccct gcggcggcgc
      661 gggctccttc aagatcgccg cggatggctc gccggccggg tagtagccca tgccccagcc
      721 atcgggcagc gtgcgtccaa cgtcgaggca atgcagcgta aatgatggtg cgagctcgcc
      781 ctcgaacgac atcgcgagca ggttcggcat cgacgctgga ctctactacg gaaccgctgc
      841 aacctctggg agggcgggga aggtctcggg ctgcaacctt ttcgaggcgg atctcctctg
      901 tatgaccgtg acgaaagcac tcgttgtcct ggtgctcctg tctgcgtgcg actcgccggg
      961 ccggcgtgcg caggatgccg cgccgatcga cgtggtcggc gagccgcagc cgctaccgcc
     1021 cgacgccgca ccgccgccgt tcgatgccgc gccgcttccc gagatggcgt gcaccgacgc
     1081 ccccgatgcg ggcatgcagt gcgcgctgcc gaagtcgacg tgtctcgatc agtactacct
     1141 cacctactac acggctggga cgtgcacgaa tagcaagtgc acgttcgagg agcactggct
     1201 ctactgctat gcggggtgct cgtcgaacat ctccggcgac gggaatgccg gatgcgaggg
     1261 cggatttacc tgacccgcac gcatctcgtc gccacgatca tcgtttgtgt cgttctgccg
     1321 ctcgcctcgt ggctcgacgg ctccggtcag ctcgcgtgga ccatgttctc gcgcaccggc
     1381 cagttccggc tcgtgatcgt tgccgacggg acgcagatga acccgaccga gatcgccgcg
     1441 gcggcgatgc cggggccgac tgcgatcgcg ctcgctggca gtgatcagct gaagcaccac
     1501 gacgtcatgc gcgcgaccgc gcgacgccat ctcgatgaca tcgccgacct cgcgtgccgg
     1561 ctgcggcccg gcgcgcagct cacggtgcga ctcgaggagc gcgtgcggcg cagcgagccg
     1621 attcgcacga gtgaagtaac acggaggtgc gcgcgatgaa gctcgcgtcg cttcgcgatc
     1681 cggtcgggtt tgtgcgcgat gcgctcgtgc tcgttcgcga cccgctcgca ctctcgcgtg
     1741 gcgcattcgc gctcgtgttc ttgctgcgca cgacgccgct gctcgcgccg tgcgatccac
     1801 ggttcttgcg cggcacgaac ggctggctcg gctggcctga cggtggctgg cacattccgg
     1861 tgcacggcct ggcactgccg gcggcggtcg tgatggcgct cgtcgtgctg cgcacgctcg
     1921 gcgcgctcgc gctgatgctc gggatctatc cgcgggtgag cggcctcgct gccggcatcg
     1981 cgggctacct cgtgctcgcg caggacgcgt tcggctacat gcatcacctg cacatgctct
     2041 atctcggcgc gatcctgttc gcgctcgtcg atcgcgacgc gccgcggtcg agtctcttga
     2101 tgctgcggct gttcgtcgcg tcgatctacg tgtgggcagt gatcggcaag ctcgcgtcgg
     2161 agtggggcac cggcgtgccg ctgactgcgt tcgtcggcaa cggcgcgctc gatcgcgatc
     2221 tcgtcggtac gctgctggcg acgcccgagc gctgcgcgat cgtggagatc gtcgtgatgg
     2281 tcatcgaggc cgcgatcgcg gcgggcttgc tctggccgcg cacgcgcaag atcgcgctcg
     2341 tatgtgcggt agcgatgcac atcgggttcg agatcattgg gcgcgtcgat tcgatcgggt
     2401 ggcagatgat ggcgctgttg ctcgtgttca ctggtgacag cgagccgcct cgtacggata
     2461 cgcgatcatc gactgaccac acatctcgtc ggtgatccgc gggcccggca gcaccggctg
     2521 gctcgtcgcg ttctgccaga tgcaatgcgt gctgatcgca tcgccagcgg cgagctgagc
     2581 gtcgactggg tacgcgtgtt gcgtgtcgaa cacccacggc acgacgtcga cgagcgcctc
     2641 actcgccgtg ctgccgcgca cgatcgcgcc gtggaactcg ctgccgatct ggtgcatgtg
     2701 cggccacgtc gacacgaggt gcagtgcggc gtcgaccgtg cacgtcgcgg tcacctccgt
     2761 gcgttgaccg ggttggatca cgggcaccgg tgcgcgcagc ggcaaccaac ctgcgcgatg
     2821 cagcgcgggc tcacgcggtg caagcgtgat cgcgtcttcg ctcgcagcgc cgtcgtcgtt
     2881 gcgcagcgcg tgtgtttgca cgatcaggtg cgtcgtgccg ccgggaatca cgagcgagac
     2941 atcggcaggc aatgcgagat cgccgccgcc cggcgcccac acgttcatcg ggaccgcgtc
     3001 gtcgggcatc tcgaggcaat cgacagggcc cgccgcgaac ggcgacggcg aggcgtacag
     3061 cgtgatgtga tgcacggcga cgggaccggt ccccggtgcg aacgtgatgc cgccgatgtc
     3121 ggcatcggcc agcggcgtcg cagcgaaacc gaagcactgg tagcgctcgg tgccgggtgc
     3181 gcccggatcg ggcgtgaagc ggagcacgac gtcggcgtcg ggcgcggcac gacagccgac
     3241 ggcgacgagc cagagccacg cgcagcgcat accgcgattc tagccggcgg cgcacaccat
     3301 gcccacgtcg agcgggctca caggcggaac gaacagcgag cggcggtgta ggggtagacg
     3361 atcgccgctt gaccacacat ctcgtcctcg ataccaaacc ccggactgat ggctcgatcg
     3421 ctggtgttct gccagatgca atgcgttggg atcacagccc ccgcagcgaa atcgaggtcg
     3481 agcggatacg tctgttggtt gtcgaagcga aactgcgcga cgtcgacgag gccgtggaac
     3541 tccgtgccca tgcgatgcat gtgcggccac gccgaaatga cgtggagaga ctccgcgagc
     3601 gcgcattgcg agtaggcctc ggcgcgcgag aacggctcga tgactggcac attggcgcgc
     3661 agctcgaacc aaccggcgcg atgctccgcg ggcgcgcggg gcacgacctc gacgacgcgc
     3721 tccaccgcgg gaccctcgtc gacgcggagg ctatgcgtct gcacgacaat ctgctgagtg
     3781 ccttcgggga tcacgagcga gatgttcgat gcaagcgcga ggtcaccgct gccggtgacc
     3841 cacacgttca tcgggaccgc gtcgccgggg aactcctggc actcgaccgg ttcagcagga
     3901 aaccgcgttc gcgaggccca caccgagacg tgatgcaacg tgaccgcgct ctcggccgcg
     3961 atgtagcgaa tcgcgccgat gtcgctgcca ccgagcaacg agacgtcgaa cgcgaagcac
     4021 tggtagagct cgctgcccgg tggcccagga tcaggcgtga agcggagcga caccgttggg
     4081 tcgtcggctt cgcaggccgc gacgagcacc aggagccagc accgacgcat gaccccagaa
     4141 tagcgtggct tcagccagcg ccgagtagct ctttgccgag cgtgtgaccg atgtcggcta
     4201 cttcgtcgac gacatgcgtg aggagctcgt gcacaccgtt gccggtgagc ggctccttct
     4261 cgcgcgccgc gacccaggtg tcgagcacgc gcagccgctc gagcgcgacg ccacctggca
     4321 actcgtgctg ctcgggcggc cggatcgcgc acagacgctc gtaggccgag tgcacgcagt
     4381 acgcgatcga acgcgggaac gcgtcctcgc tgatcaagaa cttcgcgacg gtctcgctgc
     4441 tgatgcggcc agcggcgcgc ttcatgtacg cctcggagcc cgacagcgcg cggagtagcg
     4501 acagccacag tccggtctcg acgatctcgt ggcgacgcgg caggttcgtg aacgcgtggt
     4561 gatgaacgtc gaggatgcgt gcagtctggc tgacgcgctc gagcagcacg ccgagccaga
     4621 tgaaatcgag cggctcgtcg tggagcatcg tcgagcggag cagaccgagc gtgagctgcg
     4681 tcgagcggcg aacttgctga tagaacgcct cgcgctgatt cgcgtagacc gcatgtgcgg
     4741 ccgtgctcgt gaaccacaga tgcagctcgt tgatcgtctc ccacgcttcg agcgagagga
     4801 cctcgcggac cgagcgcgcg ttccagcgac cgccgccgat cgagcgcgcg atcgatacgc
     4861 cgttcgcttc gtcccacacc atgaagcgtt gcacgacttc gccgtcgccc catgccgccg
     4921 catcgtcgtt gccatcggcg acacgcgcgc ggaacgcatc ttcctcgccc gacaccacga
     4981 cgaccggtcg ccacacttgc gcggcgggca gatcggcatc gagtgcgagg cttgcggtcg
     5041 cggcgagcat gcgtgcggtc gactcggcgc gctcgacgta gcggccgaac cagaagcagt
     5101 gatcggcgac gcgcgagatc atgtcgactc cttcatgacc cacgtgtctt tgctgccgcc
     5161 gccctgcgac gagttcacga cgtagctgcc ggcgacgagc gcgacgcgcg tgaggccgcc
     5221 gggcagtacc cacggtcctt cacgcgacgt gacgatgtac gggcgcagat cgacgcggcg
     5281 cggctcgacc acgttgcgat cgccgatcca cgtcgggcag ctcgacagct cgacgcgatg
     5341 ctgtgcgatg tagcgacggg gctcggcgat gatacgggcg cggaactcct cgcgttgctc
     5401 tgccgtcgac tgcgggccca taagcatgcc gtagccgccg gcctcgtcga ccgccttcac
     5461 gaccatctcg ccgaggtgct cgagcacgta cttgcgatcg tcgtcgcgca ggcacacgta
     5521 cgtctgcacc tgatcgagca gcggctgctc gccgagatag aactcgatca tctgcggcac
     5581 gaacggatag acggccttgt cgtcggcgac gccgttgccg ggtgcgttcg cgagcgcgac
     5641 gttgccggcc gcgtacgcgc gcatgagccc gggcacgccg agcacgctgt ccggccggaa
     5701 cacttccgga tcgaggaacg tctcgtcggt gcggcggtag atcacgtcga cgcggcgcgg
     5761 accgcgcgtc gtccggcaga acaccttgtc ctgatcgacg aacaggtcgg gcgcctgcac
     5821 gagctcgagt cccatcgtgc gcgcgaggaa cgtgtgttcg aagtacgccg agttgtacgg
     5881 cccgggcgtg agcacgaccg cgcacgtatc accgtcgggt gcgggtgaca ccgagcgcag
     5941 cgcgtcgacg agccgcgtcg ggtagtgatc gacgcgatgc acgcgcgcgg cgtcgaacac
     6001 cttcgggaac atacgcttcg acagcgaacg attctcgagc acgtacgaca cgcctgacgg
     6061 cgtgcgcaga ttgtcttcga gcacgcgcca cgtgccctgc gggtcgcgga tcagatcgat
     6121 gcccgagata tggatgcgga caccgcccgg cggcttgagg ccgcgcagca tcggccggta
     6181 tcccgccgcg ccgaggatca tctcggcggg cacgacgccg gcttcgagga tcttctgctc
     6241 gccgtagata tcgtcgagga acatgccgag ggcgcgcagc cgctgctgca agccgcgctc
     6301 gagccgagcc cagtcgccgc cggcgatcat ccgcggcacc agacagaacg gaaagatctt
     6361 ctcggtgccg cgcgaatctc catacaccga gaacgtgaca ccttgctgga gcagcgcgtg
     6421 ctccgcgagc gcctgcgcgc gtgcgagctc gtccttgccg agcttgccga gcagctgtgc
     6481 gacgcgcgcg aacggtgggc gcgcgatgcc ctgctcgaac atttcgtcgt acgtattcgg
     6541 caaagccgga tagccctcga acagtccacc ggctgttcct tggctctgac tctgctgctg
     6601 catcgctcga atggtaatac gttcgttgcc gtgatcgttc accgcccgcc atcgaagtgg
     6661 agtgacaccg acggtgtttc tgtcgtgttt ctaggcgatt ccgtcgcggc gcagatcgat
     6721 cgtgaacggg cgcgcttcgc gccagaagct cgccgcggtg acgtcgccct cacgagtcga
     6781 gatcaggatg cgttcttgtc ggcgcgtgag tgcgtcggtg tcgtcgtgcg gcaagccgtc
     6841 gtatgggccg cctcccgggc gccatgccca cagctcgatg tgctgcgcgc gaccggccca
     6901 cgtccaatcg atgacgagcg gatcggtgac cggcaggccg ggatgcaggc cgggcgatgg
     6961 ttgatagatg cggcgtcgca cgccgatcgc gcgggcaccg tcgccgatcg cgcgcagatg
     7021 cgcccacttg ccggcgatcg ctacgcgatc cggaccggga ccctcgat
//
LOCUS       DASUFX010000248        17380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1843552, whole genome shotgun sequence.
ACCESSION   DASUFX010000248 DASUFX010000000
VERSION     DASUFX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843552"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFV99_09755"
     CDS             complement(<1..197)
                     /locus_tag="VFV99_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5059633.1"
                     /translation="MAETRLRADAGSTVGTAGAKAMLAFAQSRGVDVDVVLAELGLDA
                     ETLADPEGRIPLAAFRRAWEI"
     gene            444..842
                     /locus_tag="VFV99_09760"
     CDS             444..842
                     /locus_tag="VFV99_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059634.1"
                     /translation="MKSQIIVRGRLRQRFARFLFSGAAVVSATPASAANTCEDYMPTD
                     TRVSGGGVGGRSVAAIRLAHMNPFDATHFAILAALDPSARIRRELAEALTMRFRLVGD
                     GFVLDHLVADSDPGVRAAALRACRVRGYRR"
     gene            complement(839..1432)
                     /locus_tag="VFV99_09765"
     CDS             complement(839..1432)
                     /locus_tag="VFV99_09765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5059635.1"
                     /translation="MSALLQSSILAVFLWLALGGLGLPLPEDAALLATGALIERGLVH
                     WYIALPVVVVGVLGGDAALFFLARRLGPKAYEHRMIKRALPPERREKIESAYRRYGGR
                     LVFLARHVAGLRGAVFAMAGIARMQPWRFLLCDAAAACISIPFVLSVGFFGSRHIARM
                     RAGVAHVHHYVLLVVALGVLGYVTYRHVRQLRGLRTA"
     gene            1512..2030
                     /locus_tag="VFV99_09770"
     CDS             1512..2030
                     /locus_tag="VFV99_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5059636.1"
                     /translation="MRNAILPLVFLAALPTGALANKAPASLTVKSSAFANNAAIPAEY
                     TCDGSETSPPLSWSNVPAGTKSVAILVDDPDAPMGTFTHWLVTGIPPSTTSVDKGAAI
                     PAGAMAQKNDKGKDGYAGPCPPSGRHRYRFHVYALDIAKPKSMTRSAFLAEINGHTLA
                     AGELVGTYQRQR"
     gene            complement(2027..2824)
                     /locus_tag="VFV99_09775"
     CDS             complement(2027..2824)
                     /locus_tag="VFV99_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059637.1"
                     /translation="MMAEKLVATIESRRSGARLIKFAGALDERNELGELIEKVPAGST
                     LINLSGIDSINSIGSRDWINWLSSLEARGINPVLIACSPAVVAQLNRIKNFAGNCVVK
                     SFQVPYHCDACGVDKLLLVHISELEPPYEAPVCTCDGCGAAMAFTEDPDSYFAFIPSL
                     PKPKQEVKTESQPAVEPEQLARGSNSTVVTKEHYKRISKPRVPSRDSRPSLSAFQLPD
                     AVSKRPSEGEIIVGPPRAMPLNERPYIIAIILLLFAVVGVLAYMLLG"
     gene            3008..4795
                     /locus_tag="VFV99_09780"
     CDS             3008..4795
                     /locus_tag="VFV99_09780"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEX5059638.1"
                     /translation="MKPVLVFDGECGFCRDWVERWRARTGDRVDYVPLQQPGVLQRLG
                     IPRSDALRSVQLVTPDGNRFGAADAVFRTVEYAPELRHVARFGRLPLLRTIANFVYRQ
                     IAIRRGLAASVDRLLVGKTTRPPDSRLVRNAFMRALGGVYLIAFASLGRQVKGLYGRR
                     GIEPIDDFLQFLRKRVSKRERLTQYPTVFWLDSSDAALVRACRAGEVGGALMMLGIAP
                     RTTAAVLWLLYLSFVSVGRDFLAFQWDVLLLENGLHALVLAPSRNNERPPWAAVALMR
                     LLAFRLQFESGHCKLASGDKMWRSGDACCVHFETQPLPTRGGWYAHQAPRQMKRATTY
                     AVLALELGAPWLALLPRRLRHTSFALLVGLQILIAATGNYGFFNLLTIVDNLWFLDDA
                     VLARTAERGRQTPRWRRLAITLAALPIAAISISRLVSRVFRRGRIPKPVRTLEDRLSP
                     LRSVSSYGLFAVMTTERPEIVIEGSDDAVTWREYGFRYKPGDLKRPPRWAAPHQPRLD
                     WQMWFAALGPAPSWFYQLLERLLEGAPDVLALFEHNPFPDHAPKYVRALLYDYHFTDR
                     ATQRREGTWWRRDLLGAYVPIVELADRRR"
     gene            complement(4799..5419)
                     /locus_tag="VFV99_09785"
     CDS             complement(4799..5419)
                     /locus_tag="VFV99_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5059639.1"
                     /translation="MKRISARHFLVSSAVLVALGGAGIALARPHDHKTPNAPAAGDQD
                     SWQVTVIAQKGRLGLSVLQISPELRVKLGASRDNGVLVDRVVADTPASKAGVQVGDVI
                     AEVDGDAVQSAADILDAMSERKKGEHITIALVRDGKHMDLNATLDQDPAPRGAFSAPD
                     LDAFGPHMRGMLRDMPGFGSPEMQQQLDDARKRIDELEQRLDKLEH"
     gene            complement(5477..6895)
                     /locus_tag="VFV99_09790"
     CDS             complement(5477..6895)
                     /locus_tag="VFV99_09790"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5059640.1"
                     /translation="MSIHAEPIATFEYELVDGRVGTCAHVFADSELITAIAHDHSLQQ
                     LANVTSLPGLYGCAFGMPDMHEGYGFPVGGVAATALPDGVISPGGIGFDINCGVRLLA
                     TDIDRASFAPYRERVVHDISRTIPSGYGHGGRLTLERDVLARVLEGGCRALLDELDAG
                     LPEDLDVIESGGTLAGADPTAVSERAYARGSDQLGTLGGGNHFVEVGLVDTVFDEASA
                     ARLGLYAGTITVLIHTGSRGLGHQVCTDFLALMDEAQARFGIALPDRQLACAPLSSPE
                     GRRYLAAMRAAANFAFANRQLIAHRVRAVFRRHLGVEHTLRLVYDVGHNTAKIERHGK
                     RELCVHRKGATRAFGPSSTEIPVRYRGVGQPVFIPGSMGTASYVLVGTDEAAARSFAS
                     VCHGAGRVLSRGAAKRQVMGNELRRTLESRGITIRCPSNQELAEEAPEAYKDIDRVVD
                     VVVGAGLARKVARLVPLGVVKG"
     gene            complement(6898..7335)
                     /locus_tag="VFV99_09795"
     CDS             complement(6898..7335)
                     /locus_tag="VFV99_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX5059641.1"
                     /translation="MPGTPTAAGPVVASHAFEQHTGEVKVRLEANQLSELFAEAARAL
                     CELLGSPTADAPSEWQRVTVIARDRDALLVAWLNELVARTEIDHLLYREVAITDLTEQ
                     RLDATIRGVPFRETRTAVKAATFHGLQIAVEPSGASATVVLDV"
     gene            7358..8131
                     /locus_tag="VFV99_09800"
     CDS             7358..8131
                     /locus_tag="VFV99_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5059642.1"
                     /translation="MIVVRFLGTGPAGGRPGRGRSHRRESSLAVISDAGTILIDVTRD
                     AAMQLRALDAVDLALLTHAHRDASGGLPVLDRWLRAPLPIYASHDTVRALRSRHPRLS
                     RVALHAASSQRTFAWRDWRITSLAVPHARDCTTVAWRLESPEFSIVYASDVARLTRGL
                     RELAVRCDLLVLDGAMWQRTIFTHLEIQATAPIVATWPVRRVLFTQLGRSTPDHGVLD
                     AWLHAFDPRFGAAYDGLVCTFGPRPARRMRERALHRVVA"
     gene            8128..8574
                     /locus_tag="VFV99_09805"
     CDS             8128..8574
                     /locus_tag="VFV99_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5059643.1"
                     /translation="MTAGELCIRRIVTADRDESVVDAARRMLEENVGDLVVVEQFADS
                     AIPIGIVTDRDLTLGVLSRGTPAAFELRIRDVMQTDLITVYDDEDASVALGKLERYKV
                     RRVPVVNHSGVLQGILTLDDLISWIREQLDHAAGVGARQSATVGPR"
     gene            complement(8576..8953)
                     /locus_tag="VFV99_09810"
     CDS             complement(8576..8953)
                     /locus_tag="VFV99_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5059644.1"
                     /translation="MKRIADVMTPQPWTVQLDDSLAIARRMVAERGIHHLPVLDGDDV
                     VGMVTERDLAHAAHRLGTVADVMVSAHSVPMAAPIAEVLDMMMAGGLDAVVVTSSEGV
                     EGIYTASDAVRMLLETLRRRRAA"
     gene            complement(8987..9430)
                     /locus_tag="VFV99_09815"
     CDS             complement(8987..9430)
                     /locus_tag="VFV99_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5059645.1"
                     /translation="MRVRDAMTRDPVCCTGDTPIHAVSAMMREHDIGSLPVVEDLLTR
                     FPVGVITDRDIVTRTIAAGHDPMTMLARDCMTSPAITIVEGARLHECVQLLEVAQIRR
                     VIVVDDAGACVGILAQADIARSASRRETGSLVRHVSRRTVPAFVS"
     gene            complement(9434..9670)
                     /locus_tag="VFV99_09820"
     CDS             complement(9434..9670)
                     /locus_tag="VFV99_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059646.1"
                     /translation="METRTLQRSTEYYGLGQSGYTAGRVEGDVALELELEGRNTSYPK
                     GVDEEHVLELGDDERYWGAGGRPWIPPETEARKE"
     gene            9819..10235
                     /locus_tag="VFV99_09825"
     CDS             9819..10235
                     /locus_tag="VFV99_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5059647.1"
                     /translation="MKFLVLVKASAESEAGTPPTPELLSEMMKFNEELMKAGVLLDLG
                     GLQPSSQAVRIKIDGKSRKVVDGPFSETKELVAGYWLIQVKSREEAIEWIKRCPNPTN
                     AQGEIEIRPLFEMTDFPHATTDTVERAAKIDQQIKR"
     gene            10265..10708
                     /locus_tag="VFV99_09830"
     CDS             10265..10708
                     /locus_tag="VFV99_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5059648.1"
                     /translation="MTTDTEIRRAIDAVWRIESARLIAGLARITRDVGAAEELAQDAL
                     VTALEKWPASGIPDNPGAWLMQTAKNRAIDRARRTKLVDKKHEQISAETAARPDRTAE
                     ALAAKLDDDIGDDLLRLVFTCCHPVLSREARVALTLRMLGGLTTE"
     gene            10727..11533
                     /locus_tag="VFV99_09835"
     CDS             10727..11533
                     /locus_tag="VFV99_09835"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX5059649.1"
                     /translation="MPEPTIAQRIVRAKKTLADEQVPYEVPRGDDLAPRLSSVLDVIY
                     LVFNEGYSATAGDNWMRPALCQDALRLGRILAQLAPNEPEVHGLVALMEIQASRIGAR
                     TAADGTPILLLDQNRARWDRLLIRRGLAALEQAEALPGGGEYVLQAAIAACHARAATA
                     DATDWVRIAALYAALVQLTQSPIVELNRAVAVAMAFGPAAGLAIVDSLADEPALANYH
                     LLPSVRGDLLFKLGRFAEARKELERAATMTKNERERTLLLQKAASCEAKN"
     gene            complement(11550..12020)
                     /locus_tag="VFV99_09840"
     CDS             complement(11550..12020)
                     /locus_tag="VFV99_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5059650.1"
                     /translation="MADIVKKNGNEIVQWDPMRAMRDWLRWDPFREMTPFAEFSGLER
                     FNPTFEVTENKDGYIFKADVPGVRKEDLEVTMTGNRLHISGKRESELESKQSTIYTYE
                     RQYGTFTRAFTLPEGADMDHVKSDLKDGVLTLVIPKKVEAQPKKITISSSTTKS"
     gene            12233..12874
                     /locus_tag="VFV99_09845"
     CDS             12233..12874
                     /locus_tag="VFV99_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059651.1"
                     /translation="MSSSQPWVLRAMPRAAILATAVAEVITGDLLYGGFCFVALLLTL
                     VPAIHARRIAAGVPLELELALLWLMIADMTLGNSLGLYQLHWYDKALHLSSSVLVALM
                     GFLAIYVLHMTHQTRFHAWVDSLAILLVTLGIGALWEIAEYGVDQLFARRTQGAPNLS
                     ALDDTMFDLMLDGLGGVIGAIVGPRYISRSRRGRALVTAFAGAVAARDRALPS"
     gene            12983..13939
                     /locus_tag="VFV99_09850"
     CDS             12983..13939
                     /locus_tag="VFV99_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5059652.1"
                     /translation="MPLVLRERAIPVPRENEVRIAIDACAVCRTDLHVIDGELPHPTL
                     PIVPGHQIAGVIDAVGSGVTAFAIGDRVGVPWLGSTCGVCSYCATGRENLCRSALFTG
                     YQRDGGFADACVADARFVLHLPAGYSALEAAPLLCGALIGYRALRLAGDAPRLGLYGF
                     GSAGHMIAQVARHLGREVYAFTRPGDARAQAFARSVGAVWAGDSTAHPPVALDAAIIF
                     APAGELVPLALAAVAPGGTVVCGGIHMSDIPSFPYRLLWEERTLRSVANLTRRDGEEL
                     LALAPAVGLRPHVVPYKLEDANRALDDLRAGRFDGTAVLTMH"
     gene            14053..14430
                     /locus_tag="VFV99_09855"
     CDS             14053..14430
                     /locus_tag="VFV99_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5059653.1"
                     /translation="MRLVLVAVLLFGGCSKSEPQQSPPAPAAKTKDPATARNLIASGA
                     VVIDVRTPDEYAADHLPNATNIPVEDVSARLSEIEALVSGNKAKPIVVYCAAGGRAAK
                     AKATLDGAGFSHVVNGGGLDDLQ"
     gene            14552..14998
                     /locus_tag="VFV99_09860"
     CDS             14552..14998
                     /locus_tag="VFV99_09860"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5059654.1"
                     /translation="MISPFPHRYTVRLANGQIVAPPRAPITTGPPPQFGGTDKVWSPE
                     ELLVGAALGCLWTTFAAYARRDALEYSAWSGTGNAVLEKGAPVPRFSSITLQVELDVA
                     AGDEERARRLLQTAEQRCIIANALAVPVTLEPVIRTVPPVHAASAS"
     gene            complement(15032..16054)
                     /locus_tag="VFV99_09865"
     CDS             complement(15032..16054)
                     /locus_tag="VFV99_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HEX5059655.1"
                     /translation="MIRVCAALVLAACNSPVTGEHTAAIVGGTVDVGDDAVVAIRHRS
                     RPCGDAAEASCTGTLIAPRAVLTAAHCVASESPASLVVVAGPRVDAGQRVEIETIESH
                     PDYDGLAADLAVIELAEPLPQPAVALRRVPIDASAVGAQARVVGFGVGDDGMTGTKRE
                     GTARVSEVTASTVVLAPDPALPCGGDSGGPVLLGGELASVVAYGDPACTMSNTSTRVD
                     VHFDSFIMPALARIAASTPASRPALDMACTACERTDDCPRGAECFEGTCSVLAGEAGG
                     VGSACTNDQQCGGSPCLAGLNETACRCVESCEPDGCGCKSSRAPGGELFVVAMLVWWR
                     RRYRAA"
     gene            complement(16051..16656)
                     /locus_tag="VFV99_09870"
     CDS             complement(16051..16656)
                     /locus_tag="VFV99_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEX5059656.1"
                     /translation="MRELERVAVVIALAIVAGCSKPAGPCGDGELHGQAPPKGTVTWC
                     ARSDGTKHGTFTEWHANGVVKTRGEYVNGKMEGVWATFFENTVKHTEGNYRDGRKDGT
                     WRQWDDDGTLQREEVHRVDSAESTWTAFRPTGEKWATGVSVGGRDQGPYQEWYSNGKL
                     TAQGVYEAGKRVGTWMYWNEDGTPSDTPRGEFEAKAKTSPL"
     gene            complement(16667..>17380)
                     /locus_tag="VFV99_09875"
     CDS             complement(16667..>17380)
                     /locus_tag="VFV99_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059657.1"
                     /translation="WSIAFLAVLGVAACTHDEEVATESDGFVIEQHDAHVFNATVTSA
                     GSSLRVMVNETQTDIVDVTFDFGDPVIGFHLDYTKGLGEFQPNGAPLDAAQSRLLAQL
                     YDQLTKMPEMLANKQTRVDEVAFRMANFMQIVPTGEALASHNIVAQQGWVHISCTCGN
                     QYIGSGYYRIAGRHDTGIGGSCSGGSGNGCKGRCGASCYPCAGTTAYTQDCAKHDWGV
                     GSFAAASDDFSFASNNCSC"
BASE COUNT         2908 a         5952 c         5465 g         3055 t
ORIGIN      
        1 cagatctccc aggcgcgccg gaatgcagcc agcggaatcc gcccttcagg atctgccagc
       61 gtctcggcat cgaggccaag ctccgcgagc acgacgtcga cgtcaacgcc gcgcgactgt
      121 gcgaatgcaa gcatggcctt cgcgcccgcg gttccgacgg tgcttccggc gtctgctcgc
      181 agccttgtct cggccattgt cgatgtgtca gagcattcga cgtgcccggg ctcgtgcgct
      241 ggcaaacgca ttcgcacgca agcgttgcag cacgggaata gctgaaacgc tgccgccggt
      301 acggtagccg tgctcttccc acgatgcgtg catcacgttc gtccgcgcgc tttgcgcgac
      361 cgagtccgcc gcaccgaaaa cctcgccccg ggagcaaacc cgatcgcttc tcgatcgctc
      421 ccactatcac tgcgaggatt gtcatgaaga gccaaatcat cgtgcggggc aggctccggc
      481 agcgcttcgc gcgctttctg ttcagcggcg ccgcagtcgt gtctgcgacc cccgcatcag
      541 cagcgaacac ctgcgaggac tacatgccca ccgacaccag agtctccggc ggcggggtag
      601 gcgggcgcag cgtagccgca attcggctcg ctcatatgaa tccattcgac gcgacccact
      661 tcgccattct cgcggcactc gatccaagcg cacgcattcg ccgcgagctt gccgaggcac
      721 tcacgatgcg gtttcggctc gtcggagacg gctttgtcct cgatcatctc gtcgccgata
      781 gcgatccggg tgtgcgagca gcggcactgc gagcatgccg cgtgcgaggt tatcgccgct
      841 aagcggtacg caggccgcgg agttgtcgca cgtgccgata cgtcacatag ccgagcacac
      901 cgagcgcgac gacgaggagc acgtagtgat ggacgtgcgc gacgcctgcc cgcatccgcg
      961 caatgtgccg tgaaccgaag aacccgacgc tgagcacgaa cggaatgctg atgcaggcgg
     1021 ccgctgcatc gcacagcaga aatcgccagg gctgcattcg cgcgatccct gccatcgcga
     1081 acacggcacc gcgcaaacca gcaacgtgac gcgcgaggaa caccagccgc ccgccgtacc
     1141 ggcgatacgc gctctcgatc ttctcgcgtc gctccggcgg caatgcgcgc ttgatcatgc
     1201 ggtgctcgta cgccttcgga ccgagacgac gcgcgaggaa gaacagcgcc gcgtcgccac
     1261 cgagcacgcc gacgaccacg accggcaatg cgatatacca atggacgaga ccgcgctcga
     1321 tcagcgcacc ggtcgcgagc aacgctgcgt cttcgggtaa tggcagaccg aggccaccga
     1381 gcgcgagcca gagaaacacg gcgaggatcg agctctggag caacgccgac atgcttgtca
     1441 tcggtgcaac tacgatgccc cctgctctac cgccaccacc gtaaggaaca tgcgttgcag
     1501 cacttcacgg catgcgcaac gcgatcctcc ctctagtctt cttagccgcg ctgccgacgg
     1561 gcgcgctcgc gaacaaagct ccggcatcgc tgaccgtgaa atcgtccgcc tttgcgaaca
     1621 acgcggcgat tccagccgag tacacctgtg atggttcgga aacctcaccg ccgctgtcgt
     1681 ggtcgaacgt gccggccggg acgaagagcg tcgcgattct cgtcgatgat cccgatgcac
     1741 cgatgggcac gttcacgcac tggctcgtca ctggaatccc gccgtcgacg acgagcgtcg
     1801 acaagggcgc cgcgatcccg gcgggcgcga tggcccagaa gaacgacaag ggcaaggacg
     1861 gctacgctgg cccctgcccg ccgtccggac gtcaccgcta tcgatttcac gtctacgcgc
     1921 tcgacatcgc gaagccgaag tcgatgacgc ggtccgcgtt ccttgcggag atcaacggcc
     1981 acaccctcgc agcgggcgag ctggtcggca cgtatcagcg acagcgttag ccgagcagca
     2041 tgtacgcgag cacgccgacg accgcaaaca acaacaggat gatcgcgatg atgtaagggc
     2101 gctcgttgag cggcatcgca cgcggcggac caacgatgat ctcgccttcg ctcggccgtt
     2161 tcgacacagc atccggcagc tggaatgccg acagcgacgg ccggctatcg cgcgagggaa
     2221 cgcgcggctt gctgattcgc ttgtagtgct ccttcgtgac gaccgtcgag ttcgagccac
     2281 gcgcgagctg ttcgggctcc acggcgggct gcgactcggt cttcacttcc tgcttcggct
     2341 tcggcagcga agggatgaac gcgaagtaac tatcggggtc ctcggtgaat gccatcgccg
     2401 cgccacaacc atcgcacgtg cacaccggcg cctcgtacgg aggctcgagc tcggagatat
     2461 gcacgagcag cagcttgtcc acgccgcacg cgtcgcagtg atatggaacc tggaagctct
     2521 tcacgacgca gttgcccgca aagttcttga tgcggttcag ctgcgcgacg acagccggcg
     2581 agcacgcgat cagaacggga ttgatccctc gcgcctcgag cgacgacagc cagttgatcc
     2641 agtcacgcga tccgatcgag ttgatgctgt cgatgcccga cagattgatc agcgttgacc
     2701 cagcaggaac cttctcgatc agctcgccga gctcattccg ctcgtcgaga gcaccagcaa
     2761 acttgatcaa gcgcgcgcca ctgcgtcgcg attcgatcgt agccaccagc ttctcggcca
     2821 tcatttcccc tccactgaaa cgacagtaat gacgagtaca ccataaacag atggacgcgt
     2881 ggcgtcgttt tgtcgccgaa tacccgatca ttcaccgatc tgctgtgcgt gtctgtgcga
     2941 gcagctactg atttgcgtcg gtgcacatcg cacacctctg gcgccggggt tgcttccgtt
     3001 cacctacatg aagccggtgc tcgtcttcga cggagaatgc ggattctgcc gcgattgggt
     3061 ggagcgctgg cgtgcacgca ctggagatcg ggtcgactac gtacctctcc aacagcccgg
     3121 tgttcttcag cgactcggca ttccgcgctc ggacgcgctc cgatcggtcc agctcgtcac
     3181 accggatggc aatcgtttcg gcgccgccga cgccgtgttt cgcacggtcg agtacgcacc
     3241 cgagctccga cacgtcgcgc gtttcggtcg gttgcccctg ctgcgcacga tcgcgaactt
     3301 cgtgtatcgg cagatcgcga ttcgtcgcgg gctcgccgcc agcgtcgatc gtttgctggt
     3361 cggcaagacc acgcgaccgc cggactcgcg gctcgtgcgc aatgcattca tgcgcgcgct
     3421 cggcggtgtc tatctgatcg cgttcgcgtc gctcggcaga caggtcaaag gcctgtacgg
     3481 caggcgcggc atcgagccga tcgacgactt tctccagttc ctccgcaagc gcgtgtcgaa
     3541 acgcgaacgc ctcacgcagt acccgaccgt gttctggctc gactcgtcgg acgccgcgct
     3601 tgtccgcgcc tgtcgtgccg gtgaagtggg tggcgcgctg atgatgctcg gcatcgctcc
     3661 acgcacgacg gccgcggtgc tgtggttgct ctatctctcg ttcgtgtcgg tcggccgtga
     3721 cttcctggcg ttccagtggg acgtgctgct gctcgagaat ggtctgcacg cgctcgtgct
     3781 cgcgccatcg cgcaacaacg agcggccacc gtgggccgct gtggcgctga tgcgactgct
     3841 cgcattccgg ctgcagttcg agtcgggtca ctgcaagctc gcgtcgggcg acaagatgtg
     3901 gcgcagcggc gatgcctgct gcgtgcactt cgagacccaa ccgctgccga cgcgcggcgg
     3961 ttggtatgcg catcaagcgc cgcgccagat gaagcgcgcg actacctacg cggtactcgc
     4021 cctcgagctg ggcgcgccgt ggctcgcgct gttgccgcgc cgtctccgtc acacatcgtt
     4081 cgcgttactc gtcggactgc agatcctgat cgcggccacc ggcaactacg gcttcttcaa
     4141 cctgctcacc atcgtcgaca acctgtggtt cctcgacgac gcagtgctcg cgcgcacggc
     4201 ggagcgcggt cgccaaacac cacgctggcg ccgactcgcg atcacgctcg ccgcgttgcc
     4261 gatcgccgcg atctcgatct cacggctcgt ctcgcgtgtg ttccggcgcg ggcgcatccc
     4321 gaagcccgtc agaacgctgg aagacaggtt gtcgccgctt cgttccgtga gctcgtatgg
     4381 tctgttcgcg gtgatgacga ccgagcgccc cgagatcgtg atcgaaggct ctgacgatgc
     4441 ggtgacgtgg cgcgagtatg gcttccgcta caagcctggc gatctgaagc ggccaccgcg
     4501 ctgggcagcg ccgcatcaac cgcgcctcga ctggcagatg tggttcgcag cgctcggacc
     4561 cgcgccgagc tggttctatc agctgctcga acgtctgctc gaaggcgcgc ccgacgtgct
     4621 cgcgctgttc gagcacaatc cgttccccga ccatgcgccc aagtacgtgc gcgcgctgct
     4681 ctacgactat cacttcacgg atcgcgcgac gcagcgtcgc gagggcacgt ggtggcgccg
     4741 cgacctgctc ggcgcgtacg tgccgatcgt cgagctcgcg gatcgccggc gctgacgctc
     4801 agtgctcgag cttgtcgagc cgctgttcga gctcgtcaat gcgcttacgc gcgtcgtcga
     4861 gctgctgctg catctcgggg gaaccgaagc cgggcatatc gcggagcatg ccgcgcatgt
     4921 gcggcccgaa tgcatcgaga tcgggtgccg agaaggcgcc tcgtggggcg ggatcctgat
     4981 cgagtgtcgc gttcagatcc atgtgcttgc cgtcgcgcac gagtgcgatc gtgatgtgct
     5041 cgcccttctt gcgctctgac atcgcatcca ggatgtcagc cgcggactga acggcgtcgc
     5101 cgtcgacctc ggcgatgaca tcgccgacct gcactccggc cttgctcgcc ggggtatccg
     5161 cgacgacgcg atcgacgagg acgccattgt cgcgcgaggc gccgagcttc acgcgcagct
     5221 ccgggctgat ctgcaacacc gagagtccga gcctcccctt ttgcgcgatg accgtgacct
     5281 gccaggaatc ctgatcgccg gcagccggtg cgtttggagt cttgtgatcg tgaggccgag
     5341 ccagcgcgat gcccgcgccg ccgagggcaa cgagaaccgc gctggaaacg agaaaatgtc
     5401 gtgcactgat tcgcttcatg gtcattgtct ctgcgtttgc ggcaatgacc accggctgtg
     5461 tcgatcgtat tcccggctag cccttcacga cgccgagcgg cacgagacga gcgaccttgc
     5521 gcgcgaggcc ggcaccgacg accacgtcga cgacgcgatc gatgtctttg tacgcctcgg
     5581 gtgcctcctc ggcgagctcc tgattcgatg gacaccggat cgtgatgccg cgcgactcca
     5641 gcgttctccg tagctcgttg cccatgacct ggcgcttggc cgcgccgcgc gatagcacgc
     5701 gacccgcacc gtgacacacg ctcgcgaacg agcgcgctgc cgcctcgtcg gtgccgacga
     5761 gcacgtacga cgcggtaccc atgctgccgg ggatgaacac cggctggccg acgccgcgat
     5821 agcgcacggg aatctccgtg ctcgatgggc cgaacgcgcg cgtcgctcct ttgcgatgaa
     5881 cgcacagctc gcgcttgccg tgccgttcga tcttcgcagt gttgtggccg acgtcgtaga
     5941 cgagtcgcag cgtgtgctcc acgccaaggt gacgtcggaa cacggcgcgc actcgatgcg
     6001 cgatcagctg gcggttcgcg aacgcgaagt tcgcagccgc gcgcatcgcg gcgagataac
     6061 ggcgtccttc gggagacgat agcggcgcgc atgcgagctg acgatctggc aacgcgatgc
     6121 cgaagcgcgc ctgtgcttcg tccatcagcg cgaggaagtc ggtgcacacc tggtggccga
     6181 gcccgcgcga gccggtgtgg atcagcaccg tgatcgttcc ggcgtaaagc cccagacgcg
     6241 ccgcgctcgc ttcgtcgaac acggtatcga caagcccgac ctcgacaaag tgattaccgc
     6301 cgccgagcgt gccgagctga tcgctaccgc gcgcatacgc gcgttcgctg accgccgtcg
     6361 gatccgcacc agcgagcgtc ccgcccgatt cgatcacgtc gagatcctct ggcaggcccg
     6421 catcgagctc gtcgagcagc gcgcggcagc caccttccag gacgcgggcg agcacgtcgc
     6481 gctctagcgt gagccggccg ccgtggccat agccgctcgg aatcgtgcgg ctgatgtcgt
     6541 gcacgacacg ctcgcgatac ggcgcgaagc tcgcgcgatc gatgtcggtc gcgagcaacc
     6601 gcacgccgca gttgatatcg aacccgatgc cgcccggtga gatcacgccg tcgggcaacg
     6661 cggtcgcggc aacaccgccg acgggaaagc cgtagccttc gtgcatgtct ggcatgccga
     6721 acgcgcagcc gtagagcccc ggcaacgacg tcacgttcgc gagctgctgc aggctgtgat
     6781 cgtgcgcgat cgcggtaatc agctcgctat cggcaaacac gtgcgcacac gtgccgacgc
     6841 ggccgtcgac gagctcgtac tcgaatgttg caattggttc ggcgtggatc gacatcgcta
     6901 tacatccagg acaacggtcg ccgacgcgcc ggagggttcc accgcgatct gcaggccgtg
     6961 aaacgtggcc gccttcaccg cagtgcgcgt ctcgcgaaac gggacaccgc gaattgtcgc
     7021 gtcgagccgc tgctcggtga ggtcggtgat cgcgacctcg cgatacagga gatgatcgat
     7081 ctccgtccgt gcgactagct cgttcagcca cgcgacgagc agagcatcac gatcgcgcgc
     7141 gatgaccgtg acgcgttgcc actcactcgg cgcatccgcc gtcggtgacc cgaggagctc
     7201 gcagagcgca cgcgctgcct ccgcgaacag ttcggacagc tggttcgctt ccagccgaac
     7261 cttcacctca ccggtgtgtt gctcgaacgc gtgcgatgcc acgactggac cggctgcagt
     7321 cggcgtgcct ggcatgcgga ctgctgaacc tggagctgtg atcgtcgtgc gatttctcgg
     7381 cacgggccca gcgggcggca ggcccggtcg cggtcgttcg caccggaggg agagctcgct
     7441 cgccgtgatc tccgatgccg gaacgatcct gatcgacgtc acccgcgacg cggcgatgca
     7501 attacgcgcg ctcgatgccg tggacctcgc gctgctcacg catgcgcatc gtgatgcgtc
     7561 gggcggactt ccggtcctcg accgctggct gcgcgcgcca ctgccgatct acgcgtcgca
     7621 cgatacggtg cgcgcgctgc gatcgcgtca tccacggctc tcccgcgtcg cgctacacgc
     7681 ggcgtcctcg cagcggacgt tcgcgtggcg cgactggcgc atcacgtcgc tcgcggtccc
     7741 gcatgcccgt gactgcacga cggtcgcgtg gcgactcgag tctccagaat tctcgatcgt
     7801 ctacgcctcc gatgtcgcgc gactcacgcg tggactgcgc gagctcgcgg ttcgctgtga
     7861 cttgctcgtg ctcgacggcg cgatgtggca gcgcacgatc ttcacgcacc tcgaaatcca
     7921 agcgaccgcg ccgatcgtcg cgacgtggcc ggtgcgccgc gtgttgttca cccagctcgg
     7981 gcggtcgacg ccggaccacg gcgtgctcga cgcgtggctg catgccttcg atccacggtt
     8041 cggcgccgca tacgatggac tcgtgtgcac gtttggcccg cgccccgcgc gcaggatgcg
     8101 ggaacgcgcg ttgcaccggg ttgtggcatg accgcaggcg agctttgtat ccgacgaatc
     8161 gtgacggcgg accgcgacga gtcggtcgtc gatgcagcgc gccgcatgct cgaagaaaac
     8221 gtcggcgacc tggttgtcgt cgagcaattc gcggacagtg cgattccgat cggcattgtc
     8281 accgaccgcg acctcacgct cggcgtgctc tctcgcggca cgcccgccgc gttcgagctg
     8341 cgcattcgtg acgtcatgca gaccgatttg atcaccgtct acgacgacga agatgcctcg
     8401 gtcgcgctcg gcaagctcga gcgctacaaa gtccgccggg tcccggtcgt caatcactca
     8461 ggtgtgctgc aaggcattct cacgctcgac gatctgatca gctggattcg cgaacagctc
     8521 gatcacgcgg ccggggtcgg cgcacgacaa agcgcgaccg tcggcccgcg ctagcctacg
     8581 ccgctcgccg acgcctcagc gtttcgagca gcatccgcac ggcgtccgat gcggtgtaga
     8641 tgccttcgac gccttcgctg ctcgtcacca cgaccgcatc gagaccgccc gccatcatca
     8701 tgtcgagcac ctcggcgatc ggcgcagcca ttggcaccga atgcgcggac accattacat
     8761 ccgccacggt gccgagccga tgggcggcat gtgccagatc gcgttcggtc accattccaa
     8821 cgacgtcgtc gccatcgagc acgggcaaat gatggatgcc gcgttctgcg accatccgtc
     8881 gtgcgatggc gagcgagtcg tcgagctgaa cggtccatgg ctgaggcgtc atgacgtccg
     8941 cgatgcgctt catgcacgat gaccgtgcag cgcgcgtgcc gcgacgctac gagacgaacg
     9001 caggcaccgt gcgcctggac acgtgccgca cgagactgcc cgtctcgcga cgcgacgccg
     9061 aacgcgcgat atctgcctgc gcgagaatac cgacgcacgc acccgcatca tcgacgacga
     9121 tgacccggcg aatctgcgcg acctcgagca actgcacgca ttcgtgaagg cgagcgccct
     9181 cgacgatcgt gatcgcgggt gacgtcatgc aatctcgcgc gagcatcgtc atcggatcat
     9241 gtcccgccgc gatcgtgcgg gtgacgatgt cgcgatccgt gatcacacca accgggaagc
     9301 gcgtcagcag atcctcgacg acaggaagcg agccaatatc gtgctctctc atcattgctg
     9361 acactgcgtg gataggcgtg tcacccgtgc agcacaccgg atctcgcgtc attgcatctc
     9421 ttacgcgcat cgctcactcc ttccgagctt ccgtttcagg cggaatccac ggtcggccac
     9481 cggcccccca gtaacgctcg tcgtcgccga gctcgagcac gtgctcctcg tcgacgccct
     9541 tcggatagct agtgttgcgg ccttctagct cgagctcgag ggccacatcc ccttcgacac
     9601 gccctgcggt gtagccactc tggccaaggc cgtagtactc ggtcgaacgc tgtaatgtcc
     9661 gggtctccat gcagtaccgt ggctgcatca cccgtaccgc cagccaagcc ggccagggat
     9721 tctcacggct tgaacgattc tcgaacaatg atcctgatgc gtgtcgatca gtagtcgcct
     9781 cgatcgtcgc ccatccagaa gccaacaagg agacgccgat gaagttcctc gttctagtca
     9841 aagcctccgc agagtccgaa gccggcactc cgccgactcc cgagctgctc agtgaaatga
     9901 tgaagttcaa cgaagagctg atgaaggcgg gcgtgctgct cgacctcggc ggcctccagc
     9961 catcgtcgca ggcggtccgc atcaagatcg atggtaagtc acgcaaggtc gtcgacggtc
    10021 cgttcagcga gacgaaggag ctcgttgccg gctactggct gatccaggtc aagtcgcgcg
    10081 aggaagcgat cgagtggatc aagcggtgcc cgaatccgac gaacgcgcaa ggtgagatcg
    10141 agatccgccc gctgttcgaa atgacggact tcccgcacgc gaccaccgat acggtcgagc
    10201 gcgcggcgaa aatcgatcag cagatcaagc gctagcgcgt gtcgcttgtg ctctgatccc
    10261 gtgcgtgacg accgacaccg agatccgacg tgcgatcgat gccgtgtggc gcatcgaatc
    10321 cgcccgcttg atcgcaggtc tcgcccgcat cacgcgcgac gtcggcgccg ccgaagagct
    10381 cgctcaggac gccctcgtca ccgcactcga gaagtggccg gcatcgggca ttccggacaa
    10441 cccgggtgcc tggctcatgc aaaccgcgaa gaaccgcgcg atcgatcgcg cgcgtcggac
    10501 caagctcgtg gacaagaagc acgaacagat ctcagccgag actgccgcgc gccccgaccg
    10561 cacggcggaa gcgctcgcgg ccaagctcga tgacgacatc ggcgacgatc tgcttcgcct
    10621 cgtgttcacc tgctgccatc cggtcctgtc gcgcgaagcg cgcgtcgcac tcacgctgcg
    10681 catgctcggc gggcttacaa ccgaatagat cgcgcgcgcg ttcgtcgtgc ccgaaccaac
    10741 gatcgcccag cgcatcgtgc gcgcgaagaa gacgctcgcc gacgagcagg tgccttacga
    10801 agtgccgcgc ggcgacgacc tcgcgcctcg cctgtcttcc gtcctcgacg tcatctacct
    10861 tgtcttcaac gagggctact ccgccaccgc cggcgacaac tggatgcgcc ccgccctctg
    10921 ccaagacgcg cttcgcctcg gccgcatcct cgcgcagctc gcgccgaacg agcccgaagt
    10981 gcatggcctc gtcgcgctca tggagatcca ggcctcgcgc atcggcgcac gcaccgctgc
    11041 cgatggcacg ccgattcttc tgctcgatca aaaccgcgcg cgctgggacc gcctgctgat
    11101 ccgccgcggc ctcgcagcac tcgaacaagc cgaggcgctg ccgggcggcg gcgagtacgt
    11161 gctgcaagca gcgatcgccg cgtgtcacgc gcgcgcggca accgccgacg ccaccgattg
    11221 ggtccgcatc gcggcactgt acgcggcgct cgtccagctc acgcaatccc cgatcgtcga
    11281 gctcaaccgt gccgtcgcag tggcgatggc gttcggccca gcggccggtc tggcgatcgt
    11341 cgattcgctc gccgacgagc cggcgctcgc gaactaccac ctgctgccga gcgtgcgcgg
    11401 tgatctattg ttcaagctcg gccgcttcgc ggaagcacgc aaggagctcg agcgtgcggc
    11461 aacgatgacg aagaacgagc gcgaacgaac gctgctgctg caaaaggcag cgtcgtgcga
    11521 ggccaagaac taaacgcgac cgaggtccgc tatgacttgg tcgtcgacga cgagatcgtg
    11581 atcttcttcg gctgcgcttc gaccttcttt gggatcacga gcgtcagcac accatccttg
    11641 agatcgctct tcacgtgatc catgtccgcc ccttcgggaa gcgtgaacgc tcgcgtgaag
    11701 gtgccgtact gacgttcgta cgtatagatg gtgctctgct tcgactcgag ctcgctctcc
    11761 cgcttgccgc tgatgtgcaa ccgattgccc gtcatcgtga cttcgaggtc ctcctttcgt
    11821 acgcctggaa catccgcctt gaagatgtag ccgtctttgt tctcggtgac ttcgaaggtc
    11881 ggattgaatc tctcgaggcc cgagaactcc gcgaacggcg tcatctcgcg gaacggatcc
    11941 caccgcaacc agtcgcgcat cgcgcgcatc ggatcccact gcacgatctc gttgccgttc
    12001 ttcttcacga tgtccgccat ggcttcctcc ttgtaggacg cacctggcac gcagcaagag
    12061 gcacgccacg cgcgcgctgc acttccgacg cagaacgcga attgcgtgcg ttcacgccat
    12121 cgtgaggcga acgtgctgcc ggaaatcgtg gccactgcgc cggtacgagc aatgcattgc
    12181 atcacggtat gcgcgtgcat cccggacgcc acgtcattgc ctctggggcg acatgagctc
    12241 gtcgcaacca tgggttctgc gcgcgatgcc gcgtgcagcg atcctggcaa cggcggtcgc
    12301 cgaggttatc accggtgact tgctctacgg cgggttctgc ttcgtcgcgt tgctgctgac
    12361 gctcgtgcct gcgatccatg cgcgccgcat cgccgccggc gtaccgctcg agctcgagct
    12421 cgcgctgctg tggctcatga tcgcagacat gacgcttggc aattcgctcg ggctctacca
    12481 gctgcactgg tacgacaagg cgttgcacct gagtagctcg gtgctcgtcg cgctgatggg
    12541 gttcctcgcg atctacgtgc tccacatgac gcatcagacg cggtttcatg cgtgggtcga
    12601 cagcctcgcg atcctgctcg tcacgctcgg catcggtgcg ctctgggaga tcgcagagta
    12661 tggcgtcgat cagctgttcg cgcgccgcac gcagggtgcg ccgaatctgt ccgccctcga
    12721 cgatacgatg ttcgatctga tgctcgacgg gctcggcggc gtgatcggcg cgatcgtcgg
    12781 accgcgctac atcagccgct cgcgacgcgg ccgtgcgttg gtgaccgcat tcgccggagc
    12841 agtcgcggct cgggatcgag ccctgccttc atgatctccc ttgctgcgtg atttgcgctg
    12901 ccatcgctgg cgcagtcctt gctcggacaa cgacggaacg tggagcgttg atatgcgcgc
    12961 gatggtcctg gaaggacttc acatgccgct cgtcttgcga gagcgcgcta ttccagtgcc
    13021 gcgcgagaac gaggtgcgca tcgccatcga cgcgtgcgcc gtgtgccgta ccgatctgca
    13081 cgtcattgac ggagagctgc cacatccgac gctccccatc gtgcccggcc atcagattgc
    13141 cggcgtcatc gacgccgtcg gctccggcgt gacagccttt gcaatcggcg accgcgtcgg
    13201 cgtgccgtgg ctcggttcga cgtgcggcgt gtgctcgtac tgcgcgaccg gacgcgagaa
    13261 cttgtgccgc agtgcgctct tcaccggcta ccagcgggat ggcggttttg cggacgcgtg
    13321 cgtcgcagat gcgcggtttg tcctgcactt gccagccggc tactccgcgc tcgaagcggc
    13381 gccgctgttg tgtggcgcgt tgatcggcta tcgcgcgttg cggctcgccg gcgatgcacc
    13441 gcggctgggt ctctacggct tcggctccgc cggccacatg atcgcgcagg tcgcacgtca
    13501 cctcggccgc gaggtctacg cgttcacgcg tccaggcgac gctcgggcgc aggcgttcgc
    13561 gcgcagcgtc ggcgcggtgt gggcgggcga ttcgacggcg cacccgcccg tcgcgctcga
    13621 tgctgcgatc atcttcgcgc cagcgggcga gctggttccg ctcgcgcttg cagccgttgc
    13681 acctggcggc accgtcgtgt gcggcggcat tcacatgagc gacatcccgt catttccgta
    13741 tcggttgctc tgggaagagc ggacgctgcg ctcggtcgca aacctcacgc ggcgcgacgg
    13801 cgaagaattg ctcgcgctgg cacctgccgt cgggctgcgt ccgcatgtcg tcccttacaa
    13861 gctcgaggac gccaaccgag ccctcgacga cctacgcgcg ggtcgcttcg acggtaccgc
    13921 cgtgttgacg atgcactgaa gggacgcggc cttgctcttg ccggtagctg ctggatcacc
    13981 ggcgctgcag cccattgtcg aggaggttcg tggcaagctc gtccgcgtgc tggagcgact
    14041 atcatgagcg caatgcggct ggtcctcgtc gctgtcctgc tattcggcgg ttgctcgaaa
    14101 tccgagccgc agcaatcgcc gccggcacct gctgcgaaga caaaggatcc ggcaactgcg
    14161 cgcaacctga tcgcgtccgg tgcggtggtc atcgacgtgc ggaccccgga tgagtacgcc
    14221 gcagatcacc tgccgaacgc gacgaacatt ccggtcgagg acgtctcggc acgtctttcc
    14281 gagatagagg cgctcgtcag cggcaacaaa gcgaagccga tcgtcgtcta ctgcgcagcc
    14341 ggcggacgcg ctgcgaaagc gaaggccacg ctcgacggtg caggcttctc gcacgtggtc
    14401 aacggcggcg gcctcgacga cctgcagtag cgccgtgcag cgaaactccg catctcctgc
    14461 gtgatccggc agccgtcggt gcagtcgatg cgtggacgca gagtgaatcc tttggcttcg
    14521 gcgtggctct caccgtgcag gagcgtcagc catgatctcg ccattcccac accgctacac
    14581 ggttcggctc gcaaacggac agatcgtcgc accacctcgc gcgccgatca cgaccgggcc
    14641 accaccacaa ttcggcggca ccgacaaggt gtggagcccc gaggagctcc tcgtcggtgc
    14701 ggcgctcggc tgcctgtgga cgacgttcgc ggcttacgcg cgccgcgacg cactcgagta
    14761 ctccgcgtgg tcaggcaccg ggaacgctgt tctcgaaaag ggcgcgccgg tcccacggtt
    14821 ctcgtcgatc acgctgcagg tcgagctgga cgtcgccgcc ggcgatgaag agcgcgcacg
    14881 gcgacttctg cagaccgcgg agcagcgctg catcatcgcg aatgcgcttg ctgttccggt
    14941 gactctcgag cccgtgattc gcacggtgcc cccggtgcac gccgcgagcg caagctgact
    15001 gcagtctcgg tgcgccgtga agacgtcgga atcacgcggc gcgatagcgt cgtcgccacc
    15061 acacgagcat cgcgaccaca aacagctcgc cgcccggcgc cctactcgac ttgcaaccgc
    15121 aaccatcggg ctcgcagctc tcgacgcagc ggcaggcggt ctcgtttagc cccgccaggc
    15181 acggcgagcc accgcattgt tgatcgttcg tgcatgccga gccgacgccg cccgcttcgc
    15241 cggcgagaac cgagcacgtg ccctcgaagc attcggcgcc gcgcggacag tcatcggttc
    15301 gctcgcatgc ggtgcacgcc atgtcgagcg ctggtcgcga tgccggcgtg ctcgccgcga
    15361 tgcgcgccag cgcgggcatg atgaacgaat cgaaatgcac gtcgacgcgc gtgctcgtgt
    15421 tcgacatcgt gcacgcggga tcgccgtacg cgacgacgct cgcgagctca ccgccgagca
    15481 gcaccggacc gccgctatca ccgccgcacg gcaacgccgg atcgggcgcg agcacgaccg
    15541 tcgaggcggt gacctcgctc acccgcgccg tgccctcgcg cttggtgccg gtcataccgt
    15601 cgtcgccgac gccaaacccg acgacgcgcg cctgcgcacc gaccgccgac gcgtcgatcg
    15661 gcacgcgccg caacgcgacc gctggctgcg gcaacggctc cgccagctcg atcaccgcga
    15721 ggtccgccgc gaggccgtcg tagtcgggat gactctcgat cgtttcgatc tcgacgcgct
    15781 gcccagcgtc gacacgcggc ccggccacta caacgagcga ggccggcgac tcgctcgcga
    15841 cgcagtgggc agccgtcagc acggctcgcg gcgcgatcaa cgtaccggtg caggacgcct
    15901 ccgcggcgtc gccgcacggc ctgctgcgat gacgaatcgc caccacggcg tcatcgccaa
    15961 catcgaccgt tcccccgacg atcgcggcgg tgtgctcacc ggtcaccggc gagttgcacg
    16021 cggcgagcac gagcgccgcg cacacgcgga tcatagcggg gacgttttgg ccttcgcttc
    16081 gaattcgccc cgcggtgtat cggatggggt gccgtcttcg ttccaataca tccacgtacc
    16141 gacgcgcttg cccgcttcgt acacgccctg cgcggtaagc ttgccgttgc tgtaccactc
    16201 ctggtaaggc ccctggtcgc gtccaccaac gctaacacca gtcgcccact tttcgccggt
    16261 cggacggaac gcggtccacg tcgactcggc cgagtcgacg cgatggacct cttcgcgctg
    16321 cagcgtgccg tcgtcatccc actgccgcca cgtgccgtcc ttgcgaccgt cgcgatagtt
    16381 cccctcggtg tgcttgacag tgttctcgaa gaacgtcgcc cagacgcctt ccatcttgcc
    16441 gttgacatac tcgccgcgcg ttttcaccac gccgttcgca tgccactcgg taaacgttcc
    16501 gtgcttcgtg ccgtcggaac gcgcgcacca ggtcaccgtt cccttcgggg gcgcctggcc
    16561 atgaagctcg ccatcgccgc acggtccagc aggtttgctg caaccggcga cgattgcgag
    16621 cgcgatcacc acagcgacgc gctctagctc gcgcatgggc tggaacctag cagctgcaat
    16681 tgttcgacgc gaacgagaag tcgtccgacg cagcggcgaa gctgccgacg ccccagtcat
    16741 gtttcgcgca gtcttgcgtg tacgcggtcg tgccggcgca cggatagcaa ctggcgccgc
    16801 agcgcccttt gcagccgttg ccgctgccac cgctgcagct gccgccgatg cccgtgtcat
    16861 ggcgaccggc gatccggtag taaccgctgc cgatgtactg gttaccgcac gtgcacgaga
    16921 tgtgaaccca accttgctgc gcgacgatgt tgtgcgacgc gagcgcttcg ccggtcggca
    16981 cgatctgcat gaagttcgcc atgcggaacg cgacctcgtc gacgcgcgtc tgcttgttgg
    17041 ccaacatctc gggcatcttg gtcagctgat cgtagagctg cgcgagcagg cggctctgtg
    17101 ctgcatcgag cggcgcgccg ttcggctgga actcgccgag gcccttcgtg tagtcgaggt
    17161 ggaacccgat cacgggatcg ccgaagtcga acgtgacatc gacgatgtcc gtctgggtct
    17221 cgttgaccat cacgcgcaga gaagaaccgg ccgacgtcac cgtcgcgttg aagacgtggg
    17281 cgtcgtgctg ctcgatcacg aagccgtcgc tctcggtcgc gacctcctcg tcatgcgtac
    17341 acgccgcgac gccgaggaca gcaaggaatg cgatggacca
//
LOCUS       DASUFX010000249        30471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_176792,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000249 DASUFX010000000
VERSION     DASUFX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 30471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30471
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176792"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VFV99_09880"
     CDS             <1..1394
                     /locus_tag="VFV99_09880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059658.1"
                     /translation="LLAWNLGILAQVLNAIRFAHSRGVLHRDLKPSNVMIGDFGEVYL
                     LDWGVAVSLRDDGSGRLPLAADAKQIAGTPCYMAPEMLQLDGEVPLSERTDVYLAGAV
                     LFEIIAGRPPHIGKTALAVMTSVLTSKPELAADAPTELAAICMRALDADASKRFESAD
                     ALRLALQRYLEHRGSDLILARARARLDELIEKLAAKVDNRERHHEQIYRLLGECRFGF
                     HEALAVWRDNVEARRGVVRATMAVAEYELASGDAATAVTLLSELDEPPELLARARAVA
                     AERASRRAQLEQLGKAHDLAIGQRSRGILAATIGLFYTITPLIPALRATTYPKQLLSS
                     IGAVIVISLWIWLRRAQLGMTLVNRRLLTAFVFVFVAQGLMVTGGWIAGTTMLTMQTH
                     MMFLYFLGTGLASAMIDKHLIPSALGFAAGFIVASFYPEHWMYAKSGSALAFTLNILW
                     AWRLIGHPDAETS"
     gene            1378..1914
                     /locus_tag="VFV99_09885"
     CDS             1378..1914
                     /locus_tag="VFV99_09885"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEX5059659.1"
                     /translation="MPKPPDVVMQEPAILFVGINPGKTSGKVGHHFAGPGNPFWKLLH
                     ASGLTPIELRADEDQRLAEFGYGLVNLCGRTTQTAAELTRAELARGAKQLREKVRAMQ
                     PKVVALVGVTLYPIVFRRQRGAPKLLPGPGPKPDVIAGAKVFVVPNPSGLNASFPSFE
                     AKLTWFRQLARFAGLAKQ"
     gene            complement(1921..2100)
                     /locus_tag="VFV99_09890"
     CDS             complement(1921..2100)
                     /locus_tag="VFV99_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059660.1"
                     /translation="MLLILGTTEPGQVIVARNTELRRGYECIAWRRGEPTRRLPITMV
                     LANNLVAAGAAWAAE"
     gene            complement(2133..3641)
                     /locus_tag="VFV99_09895"
     CDS             complement(2133..3641)
                     /locus_tag="VFV99_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5059661.1"
                     /translation="MIDSGLGKRRDGRRSPAVSRTSLGEGSESHPLGSSILALRDPAT
                     SSSVRRTKLVVVSNRAPGADADDTRPHVSGLANALEPALGASDGIWLGWSGREEPGDP
                     ALALDAERTPARATFHLRPEWRRHYQDGFCNQALWPLLHSFPSRVRYSTADWNTYVEV
                     NDVYARFATELVDHDGVIWVHDYHLLLVAQSLRTMGYDGAVGLFLHAPFPEPDVFATL
                     PWGDDLLAAMCAFDLVGFHTDRWADNFRACVRAAQKTLRVPKITVHPIAIDPSRFQPR
                     PCALDGDIARLRNAVGSRRIVLGVDRLDYAKGIPERLRAFDRLLAAKPEWHDRVCFVQ
                     ISVPSREHVPGYAELRHEVEALVGRINGRYGEADWVPVHYLYRSYEPVVLAQLYREAD
                     VALVTPLRDGMNLVAKEFVAAQDAENPGVLVLSQFAGASAELTDAIITNPYHPDGLAE
                     DLERALCMSRAERCERYTTLAAAVGRMSPRSWTERFTCQLHGAFRSANDEVQ"
     gene            complement(3785..4630)
                     /locus_tag="VFV99_09900"
     CDS             complement(3785..4630)
                     /locus_tag="VFV99_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059662.1"
                     /translation="MGSLQPASRSRRPAQTALWQMRSVSAQPACGCHHTLRAKPQESN
                     FFVFQAFWRPRVRGRDRQRFVHGVMVVNRRFWASAIVGDAGAMPMSDSPQLQDHQMAA
                     QFERIIRRVKSSMNYRGSYSTRDILGSLVARWLQSGEWERLKQLPPEERHIGESVRRF
                     ILDRIDQLRRRGEHDELPDDLLVVPDESTLLELVEAAELRAWIEARIADLEQGIVDPR
                     VRIPVSNPTQIGQALRLHIDGLTQRQIALQLALSLGATNKRIFEGTNYLIVLQGIEVG
                     VGGSA"
     gene            4639..5013
                     /locus_tag="VFV99_09905"
     CDS             4639..5013
                     /locus_tag="VFV99_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5059663.1"
                     /translation="MSEDLAKKCSLRFANADAAPKFALDEAELLPADRDILEQVAQCL
                     TRGPLQARTVHLIGRADPRGTDEYNLGLGTRRAESVRAYLQRLGVPASRLSPTTRGEL
                     DASGTDETTWRRDRRVDLELVN"
     gene            5016..6083
                     /locus_tag="VFV99_09910"
     CDS             5016..6083
                     /locus_tag="VFV99_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059664.1"
                     /translation="MDSSFRHRLIAANVAILFVIAAGAATAVVALQAAMRGTEQTRAI
                     DRHLALLDRLRAETRAIATSARRHVLSGDPAEQQRVLAIVDEMKTTREDLAARHAFTK
                     GPLLEADLEAYVAGVIHSLSADGDVQARLSRFEDELERVRNPLTMTFDDVAAQERSRR
                     KALHAADTLARQARWAVLAASLLGAVLVIGTLIAILRALAAHSRELLDATAMRTGLTS
                     LRREPLDAGVFVDRAVKDARDASHARGVRLRYEAQLSLRVVADHERARQVLDSLLQIA
                     IATARSGGELVVHAGAAQGGVRFAIIEANPDTDTVVGHDPALQLCSRVVEAHGGRLGI
                     QTSAISRTYWFTLPTEPVVLR"
     gene            complement(6120..7508)
                     /locus_tag="VFV99_09915"
     CDS             complement(6120..7508)
                     /locus_tag="VFV99_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059665.1"
                     /translation="MTFETKAKLGYVLLAFLLAAGMAYAIHRLSSLADEQVAYLRAEE
                     REVTLVEQMRWQSELLISAGRGYIISGDPALFAEVQHAKRRFDETTRELRSQSLSPVG
                     HELVTSVQQSAADFAGIQQQLLDARRRGENGPQLLRRFDTELLSRSRGLDEALDGLVE
                     YKESGLANQYAKESKARRRLEVGLFGLLGGLVLAGVGIAWYFARLLGRAFRTEEQALD
                     ASRDAIATRDEVMAIVAHDLRNPLGAIMMRASLMRDESDVLKMRDHAALIVNTVARMD
                     YLIKSMLDVATMDAGRFSLIPGPCAVDVLFEEIKELFEPLAASKRVTLEIERDHEGLV
                     VHADRERVLQVLSNLLGNALKFTPRGGHVTLTATRDGDRTLFSVRDTGPGIPPEHLAH
                     VFDRFWKEEEQVFGVKGTGLGLFIAKGIIEAHGGRIWAKNEPGGGARFSFTLPLAIET
                     RIPTATTVSRQR"
     gene            complement(7576..9285)
                     /locus_tag="VFV99_09920"
     CDS             complement(7576..9285)
                     /locus_tag="VFV99_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5059666.1"
                     /translation="MIGLGCMRLSTAKDRDDARSLAVLSAALDAGVDLLDTADAYAQD
                     DRDVGHNEQLIARAIAGRSVHVVTKGGLVRPDGTWVPNGRARHLAAAARASKERLGAI
                     DLYLLHAVDPQVPLTTSVRALAKLREDGIVRAIGLSNVGVHQLEQALAIAPLDAVEVE
                     LSPWHLDPLRGGLLAACKQRNIRVLAHRPFGGPAGVKRIARDEVLAQIATRLGATPYE
                     IVLAWLRQLGTVPLPGATRIETARSAAHVAELDADAVTALDAHFVAIAERPRAAAHAG
                     EREVLIIMGMPAAGKTTLALDYAARGYVRLNRDERGGTLLELARVLDRELAGGANHVV
                     LDNTYGTRALRAPVVEVAKRHDVAVRCLVTTTSLEQAQANAIARMLELHGRLLEPAEL
                     RKTQTIAPTTQFRYRREYEPPGADEGFATIEEITFTPRATAGKPALVVELDDLVWTGR
                     PIRVDHLELRPGVRDAIHRWHAAGYLIAGTTWQTSPDLDEALVQQLGVPIVIARCRHP
                     AGPPVCWCRKPLPGLALWLAKQHGLDLARSIHVGKGAADRGFATRAGLTYVDIADGFP
                     APA"
     gene            complement(9282..9797)
                     /locus_tag="VFV99_09925"
     CDS             complement(9282..9797)
                     /locus_tag="VFV99_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059667.1"
                     /translation="MISWLRDYYEAGVYPTDEGGRPISVFKDARGVRCPMAELIARSG
                     HEDLVDEIAAIDNKMRLADVKDGPVFDWMAESGLTIEEIAMVQGAMDIDYTWMRQQPN
                     IEIQRQPNGEIILARGQVRGRLETAVTALGVNGAHSLGVAAKRLAQKPATSPRATRTV
                     VATDKAPILQR"
     gene            10081..10443
                     /locus_tag="VFV99_09930"
     CDS             10081..10443
                     /locus_tag="VFV99_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059668.1"
                     /translation="MGRSLRLLVVEDHLDSAELLAELLANKGHTVRVATTASDAIAIA
                     SEQPFDIVVSDVGLPDATGYELMQQLRERYEIKGIAMTGSGRPSDIAAGKAAGFSVHL
                     TKPVSMHRLQQALEELGG"
     gene            10479..10742
                     /locus_tag="VFV99_09935"
     CDS             10479..10742
                     /locus_tag="VFV99_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059669.1"
                     /translation="MMTFNGDDSPRVGDRVFMGNELLAEIARQAPGLPPMWCYLSAGT
                     TGRLLGWRGETRAIVDIDDVDRRLVVYVSAGQVARAGMASRAA"
     gene            10815..11705
                     /locus_tag="VFV99_09940"
     CDS             10815..11705
                     /locus_tag="VFV99_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX5059670.1"
                     /translation="MRLPPVLIPLVACAACSAPASSEPRNHQAKAQAPSSPYADHIAK
                     LRKRLAKLGMRDVEIRVEDPFVVVGDGTAEDLVHDASTVRWAVEHLEADFFTARPTRV
                     LDVYLFRDADSYEAGVKRLTGDAPSTPYGFYSAANNGLFMNIGTGGGTLVHEIVHPYV
                     EADFPDAPAWLNEGLGSLFEQSATTSDGHIIGLTNWRLGGLKRAIAENRVPTFKALTH
                     LADDAFYADDTGVNYAASRYLLYYLQEQGKLREFYSAFRAARAKDPSGYATLVATLGA
                     PDMAKFEASWRQYVGKLSFP"
     gene            complement(11692..13419)
                     /locus_tag="VFV99_09945"
     CDS             complement(11692..13419)
                     /locus_tag="VFV99_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5059671.1"
                     /translation="MWRLLVLVVVVVACHAPRPRLPLVTTGEATQFVKTGRYDEAVTL
                     CHDFARVYAGVRCEEIGRTLEDRPIVMLRVSRGKGRPMILFEGGIHAGEIEGKDAGFW
                     FMRDLLDGRVAPGVLDVVDVGFIPVINPDGHERFSANNRPNQRGPVEMGFRTNGARNN
                     INRDFVKADTREVQALLGVFKRFDPIVFVDLHATDGAKFEQDIAVLVAPYARADHLDE
                     TAHALSDQLQTRMTALGHLPLPFYPSFEKDDDPMSGFAAGDAPPRFSQEYAATRSRIG
                     ILVETHSWRTYKERVQSTYHLLQALFEQALKDTPVWVKAANDADAADQSLRGGELPLV
                     YENTDHVTQIDFRGYAFERKTSDLTGGTWLVYDEAKPQIWKVPLKDEVKPKVIAHVPH
                     AGYIIDGGFAAQLAPVLDYHGIRYTKIAGQPRVDVDAYRATKVTFEKPFEGRTRVTLE
                     GAWAKETRTLDQGAIFVPLDQSRVQLIVHLLDPGGPDSFAQWGFLTNAFERKEYMEAY
                     VVEEQARQMLERDPQLRAQFDAAVAADPELAKSPERKREWFYRKHPAWDERYNLLPIY
                     RSDRVVTGN"
     gene            complement(13468..14241)
                     /locus_tag="VFV99_09950"
     CDS             complement(13468..14241)
                     /locus_tag="VFV99_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059672.1"
                     /translation="MYRDSANALDERDRAVGAILIGEALVDECQRAATLIRTSETLSH
                     NERWRTITDVHDTYPEIWRHLDRARSVLATRGANTARYDEMRPNARRAPTNAEGTDID
                     KAAIDDARRAIEDLKLAVPGADWNAINERTTHLLDKPLARAKGQRAIVLSMIVLFVFA
                     ITGWAISIIPERKVSRHELMRRELSEIAQTRKLRIEIVRVELGQRCDLERARELAKLL
                     AMDGRTIEANSFGLDYLSRCGDDEIVDNWAHAPRPPRSE"
     gene            complement(14406..14663)
                     /locus_tag="VFV99_09955"
     CDS             complement(14406..14663)
                     /locus_tag="VFV99_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059673.1"
                     /translation="MQRAAAVAMTLALAGCVTGAGIWKRDNVSLPMLVGGVAADLVVT
                     TIIASQVEDFTIGASLGTAAAVTAVDVAVGCLIGACHSLRL"
     gene            14756..15088
                     /locus_tag="VFV99_09960"
     CDS             14756..15088
                     /locus_tag="VFV99_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059674.1"
                     /translation="MDTLAIEFTGTFELALWCKLLRDRGWTGHQVAFSLGRSEGYVNN
                     LIRILERASLEVLARWKNEQATGAGLVPICATDWLMQVCVLPHDQQLGELARRVERFE
                     GRKREPGA"
     gene            complement(15149..16030)
                     /locus_tag="VFV99_09965"
     CDS             complement(15149..16030)
                     /locus_tag="VFV99_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059675.1"
                     /translation="MTRTRISACIFVVASSLAACTGTGDVEYAGEVQVASPELIAISP
                     GVQVIADADEPIFYADNDYWLYRDGYWFRSNDYRRNYVRVEFSYVPQRIRTISRPEAY
                     AHYRSHMGRDRAARTPDMRTRSLPTYQTPAQPQAQPTYPPPAPAQPGYPTPNEQPRTS
                     PASPVYQPHQPNVPGAQPPGANPTQPTEPPNPGEPARTPNDNRREPQPQRNVPPDIDH
                     TTPPATPGNPGIEHDHGMSPPPAGNANEHPTPSDRADRATPSDRADRATPPGKDKTDD
                     RTKKNDEKKDKKDKRDK"
     gene            16097..16963
                     /locus_tag="VFV99_09970"
     CDS             16097..16963
                     /locus_tag="VFV99_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5059676.1"
                     /translation="MSEVDDGKEDGKKKRKLRTLTGISVDEFRHPLDQRATEALKKVP
                     GLDKLIAKILEIGLERMLYIKNIGSNLRVTPKMFPRLHKSLTWATKILDVPEPEMYVT
                     TDPRPNAFTFGHTKPFITLTTGLIDMMTDEEVFFVIGHEVGHIKGGHVLYMTMAYNIA
                     AILELVGQFTLGIGALVGRGLEYALLEWVRCAELTSDRAALLCVQDLEPARSTFMKLA
                     GGTTRLAAEIDRDEFLRQIREYEDVDHSTLDRAYKLLLTIDRTHPFAMQRAKEMETWY
                     ESGYADLMARHP"
     gene            17053..17292
                     /locus_tag="VFV99_09975"
     CDS             17053..17292
                     /locus_tag="VFV99_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059677.1"
                     /translation="MRNTDSIASLDLELLTTVTGGCGSKKQQQCAPPQAPPPQQSEGG
                     GTNVVVATGTQGGAMIQQALQGGAMPGAPQPPRMI"
     gene            17344..18942
                     /locus_tag="VFV99_09980"
     CDS             17344..18942
                     /locus_tag="VFV99_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5059678.1"
                     /translation="MRDRIIANDSGSTRSTWMTEWVPDFSAEIAEGEHPEVCVIGAGI
                     AGLSVAVALVREGVDVLVLDQGPIGGGQTARTSAHVASALDDYFHVLERRFGRDGAKL
                     CYESHAAAIDEIENNARRFGIDCDFRRVDGYLWAGSDQHHAMLEKELAAAKRVGLGVD
                     EIARAPLPFDTGPCLRFARQAEFHPLKYLRGIAQAIIAGSGRIHTGVHVVGVKSGTPV
                     EITLQGGRTLRATAVVDATQMAITSRFNMPLREAAYRTYIVALAIPHDYVPHGLYWDT
                     LDPYHYVRVAADGDGREILIVGGEDHRVGQGDPEIHFPRLEAWARDHFPDAGPAVATW
                     SGQVQEPSDGMAYIGKLPGHPHIYVVTGDSGNGITHGTVAGLLLPSLIRDRPHPWQHI
                     YAPNRSRLRGAGELVKEAMHSNGPYVDWMRGGDVSNLEDIRPGHGATIRRGLHVIAAY
                     KDERGQCHLMNARCPHLSGVVRWNEVEKTWDCPVHGSRFDCEGRVLNGPASVDLGEAT
                     ADIEAPIQIPEPVFGEEPFTLKPV"
     gene            complement(19087..19671)
                     /locus_tag="VFV99_09985"
     CDS             complement(19087..19671)
                     /locus_tag="VFV99_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059679.1"
                     /translation="MIGIPLAIASFAYGEWAAHRYLLHWRGRNKDSVFAFHFHVHHQK
                     VRRHGGYDPDFEGPVWSTPTQLGEAIGLTVIGLAHAPLFPIAPFYTATTWYLLAKYRR
                     DHRRCHIDPEWGRTHLAWHYDHHMGPNQHKNWGVTFKWFDVLRGTREEYVGTPKELAD
                     RTRNRERARAAKDGTAARPPRRVRWRDVLKRRAA"
     gene            complement(19748..20365)
                     /locus_tag="VFV99_09990"
     CDS             complement(19748..20365)
                     /locus_tag="VFV99_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059680.1"
                     /translation="MTLFVAACISNDPAGGEHFDSVRDRLADGPTRLYVGADGSTGSI
                     TAKRKTSDGWAEGVTQVTIDGGEVLAQVDSHGALTVNRLTVSLAPIEIPEDVFKKPAT
                     LDDVRVSLTAPAHADVAWTDDNDATLTLPLMLDFDWSIHINGSNTPLATQHLPPVSID
                     VMLTGAGDHIDASLSLQAMGELWNWAGLLEMTKMDLSLAAGTVEE"
     gene            complement(20596..20970)
                     /locus_tag="VFV99_09995"
     CDS             complement(20596..20970)
                     /locus_tag="VFV99_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059681.1"
                     /translation="MRAIVFVLLAGCGAQPNQPVLANAPKPDPGAVAGAAAAAAAAIT
                     LASPDSHTPEKRQNEEKKPVKVKENVSAGALDRLDASEAAAKNQPKQDDADAKPGGAK
                     QQPAGAKTEPEKPKGALDFGQP"
     gene            20997..21977
                     /locus_tag="VFV99_10000"
     CDS             20997..21977
                     /locus_tag="VFV99_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX5059682.1"
                     /translation="MRAIAITKPGGPEVLALVEKPQPEPARGEVRVRVRATAVNRADL
                     LQRMGGYPAPSDSPPDIPGLEIAGEVEALGPGSARFAVGDRVFGLVGGGGYAEQIVSH
                     ERALAKIPDGMSFEDAGAVVEAFVTAHDAMVTQAQLKSGEPLLIHAVGSGVGTAAAQL
                     GRALGAFVIGTARTQDKLDRARALGMDAGVIALGGKFADAVRRVQPQGAAVILELVGG
                     GYLAEDLRCVQTLGRIALVGLMAGAKTELDLGLVLTKRVRILGTVLRARPLEEKIAAM
                     RAFETQVVPLLARGVVKPVIDCVMPLADAAKAHERMASNAGFGKIVLRVD"
     gene            complement(22004..22186)
                     /locus_tag="VFV99_10005"
     CDS             complement(22004..22186)
                     /locus_tag="VFV99_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059683.1"
                     /translation="MHRRWSFVLVMMFAASCEQECKQSERSNCCKVCTTGKPCGDTCI
                     EETDTCHQVGGCACAK"
     gene            complement(22194..22727)
                     /locus_tag="VFV99_10010"
     CDS             complement(22194..22727)
                     /locus_tag="VFV99_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059684.1"
                     /translation="MKLLAVAAVTIAASAFARPAAACGVPDFGAMLADVFKVETTQVH
                     TPIVIVGGGSSTEGSVGSFAIGYAWGEKQTDWLFPGSSVTRVLLDTRTDAGETTAVAA
                     TYGWYQNRVGSAGLDLGAEAELTGLRGVGPTARLTLGLHGVALQLTGGVMFGGEAPRY
                     EGSAQLVLEVMDMTGVI"
     gene            22904..24640
                     /locus_tag="VFV99_10015"
     CDS             22904..24640
                     /locus_tag="VFV99_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5059685.1"
                     /translation="MVHPASARLIETIRASIIGDDHALEGPYGPRRVTYADYTASGRS
                     ITFIEEFIREQVMPLYANTHTETSGTGAQTTQFREDAREVIRRAVGGNDDDCVIFCGS
                     GATGAINKLIDILNLRVPNDLDARYELTSRIPEAERPVVFIGPFEHHSNELPWRESIA
                     DVVVIHEDGDGHVDLAHLEQELVRYAGRSLKIGSFSAASNVTGIATDTRAISTLLHQH
                     NALSFWDYAAAGPYVQISMHEDDTLAYKDAVFISPHKFIGGPQTPGVLVVKRKLLVNT
                     VPACPGGGTVAYVNQEEHRYVTDPVIREEGGTPAIIESVRAGLVFQLKEAVGADAIRE
                     REVDFIQRAIASWSANPNIAILGNKSAWRLSIVSFIVRHREAYLHHNFIVALLNDLFG
                     IQSRGGCGCAGPYGHRLLGIDVETSKEFEREIVRGCEGIKPGWVRVNFNYFLSETQFQ
                     FLLDAIHLVANDGWRLLPHYTFDPDTGEWRHRAHSRKHVMSLAEVSYRTGKMEYRSRH
                     STEPEWALPNYIDEAKKIISATADEFRTLQIKEAERSEDFEELRWFPLPSEVLEELRG
                     QPTGRHAALLRP"
     gene            complement(24664..25251)
                     /locus_tag="VFV99_10020"
     CDS             complement(24664..25251)
                     /locus_tag="VFV99_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059686.1"
                     /translation="MRAIIALALLCAPVVAHADSYVELAGGISIPAGDDDYTNNVETS
                     PVLALRAGAFPKELGLFFGAEWAPMNTDYENNQFADVSAHRFRLMAGGIIHHNISNLL
                     AFSARGGIGADIAYVNIDSVVGDQSDTDVGLGFEFAGGLWAKVGGLEVGGELAIPIST
                     HDDDNPGDFIPLDYTAYDVQLLFAVRFVSRSGGGD"
     gene            25397..25684
                     /locus_tag="VFV99_10025"
     CDS             25397..25684
                     /locus_tag="VFV99_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059687.1"
                     /translation="MKTISEVELANVTGGKVPDATSVTPGSSSSSNNDAVMSALNGIQ
                     SSLKDLQNPQGLFGGNNGLLFMTMAFAMSRRSEVVVYGGGPRRGYSWRAYW"
     gene            complement(25694..26023)
                     /locus_tag="VFV99_10030"
     CDS             complement(25694..26023)
                     /locus_tag="VFV99_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HEX5059688.1"
                     /translation="MNDRVGRDEHALVCVVCGHRISDDDHRIEMAGAHEHTFVNPGGF
                     VHHIGCFNAAPGCLYLGPTESAFSWFPGWTWQVAACGNCRAHVGWIYRNAGEQFHGLI
                     VAAIRRA"
     gene            26094..26564
                     /locus_tag="VFV99_10035"
     CDS             26094..26564
                     /locus_tag="VFV99_10035"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5059689.1"
                     /translation="MSMAADRILPLCELLLGAAYADKELKDQEKDEVRSLLEGLAGEL
                     PTEVEMKIASFDPGSFKLETSAAVFKADSEDDRKKLLYLVAAVNEADEEIDFAEDEYL
                     RDLASALALPKSALDGLTVEIETEELKETFKQVRKGPPPPPPSAKSKSVDIDMD"
     gene            26787..27086
                     /locus_tag="VFV99_10040"
     CDS             26787..27086
                     /locus_tag="VFV99_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059690.1"
                     /translation="MHNRKDLQLCRQVYDALTYALADIEDLMILDLVLASVVPAPSAA
                     RLQVTLVPAHDNVDPEEALARVKEVADELREEVAAEVTRKRAPELVFRIGHRDEL"
     gene            complement(27175..27627)
                     /locus_tag="VFV99_10045"
     CDS             complement(27175..27627)
                     /locus_tag="VFV99_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX5059691.1"
                     /translation="MADKPEMNRQDAADAKLRELLHPEPTEELDRCANTVIGAAIDVH
                     RALGPGFLEQVYESAMMVELQLRGIPVRRQVPLQLEYKGHRLPSMQVDLLVDDILIVE
                     LKAGESLAPIHMAQVLSYLRAGAFQLGLLINFNVPLLRQGIRRVIFSG"
     gene            complement(27647..28009)
                     /locus_tag="VFV99_10050"
     CDS             complement(27647..28009)
                     /locus_tag="VFV99_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059692.1"
                     /translation="MNLACPECGHRQADGDRCATCSYDGLLDLENERHVELLRDIDRR
                     RRDKHTDRVRLVSVGITLAIVFALWLVPGYWKARGLYYPGLPLLADQFFFMIVLALGI
                     SKLLARAEPPSRFPYLDE"
     gene            complement(28006..28446)
                     /locus_tag="VFV99_10055"
     CDS             complement(28006..28446)
                     /locus_tag="VFV99_10055"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5059693.1"
                     /translation="MALKRTSTAVWHGKGPTGSGTISTLSGAFKEQPYSVNTRFTSED
                     GKAGTNPEELIAAAHASCFTMALSFQLTNANFDPTELKTVATVSMEKQEPGWTITNIH
                     LDVVGKVPGVSADQFQTLAETAKKGCPISRALAATPITMSAKLA"
     gene            complement(28495..29175)
                     /locus_tag="VFV99_10060"
     CDS             complement(28495..29175)
                     /locus_tag="VFV99_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059694.1"
                     /translation="MSGALLATIGLAAATALVSGCGAGSTYRTTRIAPPGHTEWLFGA
                     QVSGAGTVGIDEGGGDGGVAPLPELAVAARRGLDERFEVQVNTTFLPTPVASTGSIEL
                     AGKMRVAERGRWSLAVGSGVGYRLTAVGGAHIEDIYASVPVIGGIELGRHQLVLSIDG
                     GYHRLYSSGANPVDIPFIGESIGFRWQVGRRWAVLPEIGGAWSPTANFMTEDSRLFHF
                     GIAALWTR"
     gene            complement(29205..>30471)
                     /locus_tag="VFV99_10065"
     CDS             complement(29205..>30471)
                     /locus_tag="VFV99_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059695.1"
                     /translation="ALADDGTSGVATPTWYRDIEPLVTTTCAGCHTDSGIAPFALTTY
                     QDFVPIREAITEAVDERRMPPWQPADCCNHYRWDRSLSDAERNTLLRWLRSGMALGDI
                     ADQMPVPAPPTGLPRVDLTATMHVPFIPQPKIGADEIRCFLLDHDDIPRDRYITGFDF
                     KPGVRGEVHHIIVAAVDEDHAAELAKRDGEDGRPGWDCWGDGASEFGSDAQYIGGWQP
                     GVLPRLLPDGVGRKLPAHTRILLNVHYDTGHGIAADQSSIDIMLEDHVERVERGTAAG
                     NPLWFIGDGMEIPAGAPDTKVWFAWDPTVLTKGEPIHIHNVMLHMHELGSIGRVAILR
                     ADGSTECLLNIPDWDFHWMADYYFETPVVLSKGDQLYVECHWDNTQANQKIVNGHQQT
                     PKTIHWGTDQEMCGAVVTYSEAVGSAVGS"
BASE COUNT         5211 a         9904 c        10048 g         5308 t
ORIGIN      
        1 atttgctcgc gtggaacctc ggcattctcg cgcaggtact caacgcgatt cgcttcgcgc
       61 acagccgcgg cgtgctgcat cgcgatctca agccgagcaa cgtgatgatc ggcgacttcg
      121 gcgaggtcta cctgctcgac tggggtgtcg cggtaagtct gcgcgacgac ggctcgggtc
      181 ggctgccgct cgctgccgac gcgaagcaga tcgccggcac gccttgctac atggcacccg
      241 agatgctgca gctcgacgga gaggtgccgc tatcggagcg cactgacgtc tatctggcgg
      301 gtgcagtgct attcgaaatc atcgccgggc ggccgccgca catcggcaag accgcgctcg
      361 ccgtgatgac gagcgtactg acctcgaaac ccgagctggc ggccgacgcg cccaccgagc
      421 tcgctgcgat ctgcatgcgt gccctcgatg cagatgcgag caaacgcttc gagtcggccg
      481 atgcgctgcg gctcgcgttg cagcgttacc tcgagcatcg cggctcggat ctgattctcg
      541 cacgggcgcg cgcgcgcctc gacgagctga tcgagaagct cgccgcgaag gtggataatc
      601 gcgaacggca tcacgagcag atctatcggc tgctcggcga gtgtcggttc ggatttcacg
      661 aggcgctcgc ggtctggcgc gacaacgtgg aggcgcggcg cggtgtcgtg cgcgcgacga
      721 tggcggtcgc cgagtacgag ctggcgagcg gagacgcagc gacggcagtc acgctgctga
      781 gtgagctcga cgagccaccg gagctactcg caagagcgcg cgcggtggcg gcagagcgag
      841 cgtcgcgtcg cgcgcagctc gagcagctcg gcaaggcgca cgatctcgcg attggccagc
      901 gcagccgcgg catcctggcc gcgacgatcg gcctgttcta cacgatcacg ccgctgatcc
      961 ccgcgctccg cgcgacgacc tacccgaagc agctgctgtc ctcgatcggc gcggtgatcg
     1021 tgatctcgct atggatctgg ctgcgtcgcg cgcagctggg catgacgctc gtcaatcgcc
     1081 ggctcctcac tgcgttcgtg ttcgtgttcg tggcgcaagg actgatggtc accggcggct
     1141 ggatcgccgg cacgacgatg ctgacgatgc agacccacat gatgtttctc tacttcctcg
     1201 gcaccgggct ggcgtctgcg atgatcgaca agcatctcat cccgtccgcg ctcggctttg
     1261 cggccggatt catcgtcgcc tcgttctatc ccgagcactg gatgtacgcg aagagcggat
     1321 ccgcgctcgc gttcacgctc aacatcctgt gggcgtggcg actgatcgga catcccgatg
     1381 ccgaaacctc ctgacgtcgt gatgcaggag ccggcgatct tgttcgtcgg tatcaatccc
     1441 ggcaagacat cgggcaaggt cggccatcac ttcgcgggac cgggcaatcc gttctggaag
     1501 ctgctccatg ccagcgggct cacgccgatc gagctgcgcg cagacgagga tcagcggctc
     1561 gccgagttcg gttacgggct cgtcaacttg tgtggccgca cgacgcagac cgccgcagag
     1621 ctgacgaggg ccgagctcgc gcgtggcgcc aagcaattgc gcgagaaggt gcgcgcgatg
     1681 cagccgaagg tcgtcgcgct cgtcggcgtg acgctctatc cgatcgtgtt tcggcgccag
     1741 cgcggcgcac cgaagctgct tcccgggccg ggaccgaagc ccgatgtcat cgcgggcgcg
     1801 aaggtgttcg tcgttccgaa tccaagcggg ctcaacgcat cgtttccgtc gttcgaggcg
     1861 aagctcacgt ggtttcgtca gctcgcgcgg tttgccggcc tcgctaagca gtaggcgcga
     1921 tcactccgct gcccacgctg cgcctgcggc gaccaggttg ttcgcgagga ccatggtgat
     1981 cggtagtcgg cgcgtgggct cccctcggcg ccacgcaatg cactcgtagc cacggcgaag
     2041 ctcggtgttg cgcgcaacga tcacttgtcc cggttccgtg gttccgagaa tcagcaacac
     2101 gtcgatggca tcacggtcga gagacaaacg aactactgga cctcgtcgtt agcgcttcga
     2161 aaggcgccgt gcaattggca cgtgaaccgc tcggtccacg agcgcgggct catccgacca
     2221 actgcggcgg caagcgtggt gtagcgttcg catcgttcgg cgcgcgacat acacaacgcg
     2281 cgttcgagat cctccgcgag accgtcggga tggtacgggt tcgtgatgat cgcgtcggtg
     2341 agctcggcgg aggctccggc gaactgcgag aggacgagca cccccggatt ctccgcgtcc
     2401 tgggcggcga cgaactcctt ggcgaccagg ttcatgccgt cgcgcaacgg ggtcacgagt
     2461 gcgacgtctg cttcgcgata gagttgtgcc agcacgacgg gctcgtacga tcgatagagg
     2521 tagtggaccg gcacccagtc cgcttcgccg tagcgtccgt tgatgcggcc gacgagcgct
     2581 tcgacctcgt gccggagctc ggcgtaacca gggacgtgct cgcgcgaggg caccgaaatt
     2641 tgaacgaaac acacgcggtc gtgccattcg ggcttggcgg ctagcaggcg atcgaacgcg
     2701 cgtaagcgct ctggtatccc cttcgcatag tcaaggcgat cgacgccgag cacgatccgc
     2761 cgcgagccaa ccgcgttgcg caaacgcgcg atgtctccgt cgagcgcgca cgggcggggc
     2821 tggaaccggc tcggatcgat cgcgatcgga tgtacggtga tcttcgggac gcgaagcgtt
     2881 ttctgcgcgg ctcgcacgca cgcgcggaag ttgtcggccc accgatcggt atgaaaaccg
     2941 acgagatcga acgcgcacat cgcagcgagg aggtcgtcgc cccacggcaa cgtcgcaaat
     3001 acatcgggtt cggggaatgg cgcgtggagg aacaggccga ccgctccgtc gtagcccatg
     3061 gtccgtaacg actgcgcgac gagcaagaga tgatagtcgt gtacccagat cacgccgtcg
     3121 tggtcgacga gctccgtggc gaaccgcgca tacacatcat tcacctcgac gtacgtgttc
     3181 cagtctgccg tgctgtaacg cacgcgactc gggaacgagt gcaggagcgg ccacagcgct
     3241 tggttgcaga agccatcctg ataatgccgc cgccattccg ggcggagatg gaacgtggcg
     3301 cgtgcgggtg tgcgctcggc atcgagcgcg agagcaggat cccccggctc ttcgcggccg
     3361 ctccagccga gccagatgcc gtcggatgcg ccaagtgccg gttcgagcgc gttggccagc
     3421 ccgctcacat gagggcgcgt gtcatcagcg tccgccccgg gcgcgcgatt ggatacgacc
     3481 acgagcttcg tgcgccgtac ggaagaagag gtggcgggat cccgcagtgc aaggatggat
     3541 gagccgagag gatgcgactc actcccttcc ccaagggagg tgcgggaaac cgccggtgag
     3601 cgcctgccat cgcgccgttt gccgagtccg gaatcgatca cgcaagccgc ctcacactca
     3661 cgaggacggc ggcgagccgg gtttgttcac gaccccgcgg acttgcgtag catgtcggtc
     3721 cggctgcggt cgtccacaac cttgcgaaat tgcttctgct gggcgtcacg caagcgcgtc
     3781 accatcacgc gcttcctccg acgccgacct cgataccttg gagcacgatg aggtaattcg
     3841 ttccttcgaa gattcgcttg ttggtggccc cgagcgagag cgctagctgc aacgcgatct
     3901 ggcgttgcgt cagaccgtcg atgtgaagcc gcaaggcctg gccgatctgc gtcggattgg
     3961 agaccggtat ccgtacgcgt gggtcgacga tgccttgctc gagatcggcg atccgtgcct
     4021 cgatccacgc tcgcagctct gccgcctcga cgagctcgag cagtgtggat tcgtccggca
     4081 cgaccagcag gtcatcggga agctcgtcat gctcgccacg ccggcgcagc tgatcgattc
     4141 gatcgaggat gaagcggcgc acactctcac cgatgtggcg ttcctccggt gggagttgct
     4201 tcaatcgctc ccattcgccg ctctgcaacc agcgcgcaac gagcgagccg aggatgtctc
     4261 gagtgctata cgaaccgcgg tagttcatcg agctcttgac acgacggatg atccgctcga
     4321 attgagcggc catttggtgg tcctggagtt gcggcgagtc cgacatcggc atggcgccgg
     4381 cgtcacccac gatggcgctg gcccaaaatc gtctgttcac gaccataaca ccgtgaacaa
     4441 agcgttggcg gtcccgtcct cgtacccgag gcctccaaaa tgcttggaac acaaagaagt
     4501 ttgattcttg cggtttcgct cgcagcgtgt ggtggcagcc acaagccggc tgcgccgaaa
     4561 ccgagcgcat ctgccacagt gccgtctgcg ccggccgccg cgagcgcgaa gccggttgca
     4621 gcgagcccaa acgttgctgt gtccgaggat ctcgcgaaga aatgctcctt acgcttcgcg
     4681 aacgcagacg ctgcgccgaa attcgctctc gacgaagccg agctgcttcc cgccgatcgc
     4741 gacattctcg agcaggtcgc gcaatgcctg acccgcgggc cactgcaagc acggaccgtg
     4801 cacctgatcg gccgcgccga tccgcgtgga acggacgagt acaacctcgg gctcggaacg
     4861 cggcgcgcag agagtgtgcg cgcttacttg caacgactcg gtgtgcccgc aagtcgactg
     4921 tcaccgacga cacgcggtga gctcgatgcc agcggcaccg acgaaaccac ctggcggcgc
     4981 gatcgccgag tcgatttgga gctcgtgaac tgagcatgga cagctcgttc cggcaccgat
     5041 tgatcgccgc gaatgtcgcg atcctatttg tgatcgcggc aggtgccgcg accgcggtcg
     5101 tcgcattgca agcggcgatg cgcggaacgg aacagacgcg agcgatcgac cgtcacctcg
     5161 cgcttctgga tcggctgcgc gcggagacgc gggcgatcgc gacgtcggca cggcggcacg
     5221 tgttgagcgg cgatcctgcg gaacagcaac gtgtgctcgc gatcgtcgac gaaatgaaga
     5281 ccacgcgcga ggacctcgcc gcccgccacg catttacaaa gggaccgctg ctcgaagcgg
     5341 atctggaagc gtacgtcgcc ggtgtgatcc actcgctgtc ggccgatggc gacgtgcaag
     5401 cgcggctatc gcgattcgag gacgagctgg agcgcgtgcg taatccgctc accatgacgt
     5461 tcgacgatgt tgctgcgcag gagcggtctc ggcgcaaagc gcttcatgcg gcggacacgc
     5521 ttgcgcggca ggcccgctgg gctgtgctgg cggcgagcct gctcggggcg gtgctcgtga
     5581 tcggcacgct gatcgccatt cttcgcgcgc tcgctgcgca ttcgcgcgag ttgctcgacg
     5641 ccaccgcaat gcggaccggt ttgacctcgc tacgtcgcga gccactcgat gccggggtct
     5701 tcgtcgaccg cgcggtcaag gacgcgcggg acgcatcgca tgcacgggga gtgcggctgc
     5761 ggtacgaagc acagctctcg ctacgagtcg ttgccgatca cgaacgcgcg cggcaggtac
     5821 tcgattccct gctccagatc gcgatcgcta ctgcccgctc gggcggtgag ctcgtcgtgc
     5881 atgccggtgc tgcccagggt ggtgtgcggt tcgcgatcat cgaagcgaac ccggatactg
     5941 acaccgtcgt cggtcacgat cccgcgctcc agttgtgcag ccgtgtcgtc gaagcgcatg
     6001 gtggacgact cggtatccag acctcggcga tcagtcgcac gtattggttc acattgccaa
     6061 cggaacccgt ggttctccgg tgaacgcgca aacattcaaa tacgcgcggt gctacacgct
     6121 caacgctggc gactcacggt cgtcgcggtc ggaatccggg tctcgatggc aagtgggaga
     6181 gtgaacgaga accgcgcgcc gcctccgggc tcgtttttcg cccagatccg accgccatgc
     6241 gcctcgatga tgcccttggc gatgaacagg ccgagcccgg tccccttcac gccgaagacc
     6301 tgctcctcct ctttccagaa tcgatcgaac acgtgcgcga ggtgctccgg cgggatgccg
     6361 gggccggtgt ccctgacgga aaatagcgtg cgatcgccgt cgcgcgtggc cgtgagcgtc
     6421 acgtgaccgc cgcgcggcgt gaacttgagc gcattcccga gcaagttcga gagcacctga
     6481 agaacgcgtt cgcgatccgc gtggaccacg agaccctcgt gatcgcgctc gatttccagc
     6541 gtcacgcgtt tcgaggcggc gagaggctcg aacagctcct tgatttcctc gaacagaacg
     6601 tcgacggcgc aaggaccagg aatgagcgag aatcggccgg cgtccatggt tgcgacgtcg
     6661 agcatagatt tgatcaagta gtccatccgc gcgacggtgt tcacgatcag ggcggcgtgg
     6721 tcgcgcatct tgagtacgtc tgactcgtca cgcatcagcg atgcccgcat catgatcgcg
     6781 ccgagagggt tgcggagatc atgcgcgacg atcgccatca cttcgtcgcg tgtcgcgatc
     6841 gcgtcgcgcg aggcgtcgag cgcttgttct tctgtacgga acgcgcgccc gagcagccgc
     6901 gcgaagtacc aggcgatccc gacgccggcg agcacgagac cgccgaggag cccgaacagg
     6961 ccgacctcga gccgccggcg ggctttgctt tctttcgcgt actggttcgc gagcccggat
     7021 tctttgtact cgaccagtcc gtccaaggcc tcgtcgaggc cgcgacttcg tgacaacagc
     7081 tcggtgtcga accgacggag gagttgcggg ccgttctcac cgcggcgtcg cgcatcgagg
     7141 agctgctgtt ggatacccgc aaagtcggcc gcggactgct gtacggacgt caccagctcg
     7201 tggccgaccg gcgacagcga ctgactgcgc agctcgcggg tggtttcatc gaagcggcgc
     7261 ttcgcgtgct gcacttcagc gaacagtgcc ggatcacccg agatgatgta gccacgcccg
     7321 gccgagatca gcagctcgct ctgccagcgc atctgctcga cgagcgtcac ctcgcgttcc
     7381 tcggcgcgta agtacgcaac ctgttcgtcg gcgaggctcg agagtcggtg aatcgcatag
     7441 gccatgcccg ctgcgagaag gaatgccaac aggacgtagc cgagctttgc cttggtctcg
     7501 aatgtcatga cgaaatgctt gcgccgggcg cgagcctcgt cttcgaaatc ttcaagaagc
     7561 aggccggcgc agctgtcacg ccggtgccgg gaatccatct gcgatgtcga cgtaggtcag
     7621 gcccgcacgc gtcgcgaaac cgcgatcggc cgcgcccttg ccgacgtgga tgctgcgcgc
     7681 gagatcgagt ccgtgttgct tggcgagcca tagcgcgagc ccggggagcg gcttgcggca
     7741 ccagcacaca ggcgggcccg ctggatgccg acagcgagcg atcacgatcg gcacaccgag
     7801 ttgttggacg agcgcctcgt cgaggtccgg cgaggtctgc cacgtcgtgc ccgcgatcag
     7861 atagcccgcc gcgtgccagc ggtggatcgc gtcgcgtaca ccgggccgca gctcgaggtg
     7921 atcgacgcga atgggtcgcc cggtccacac gaggtcgtcg agctcgacga cgagcgccgg
     7981 tttgcctgcc gtcgcgcgtg gcgtgaatgt gatctcttcg atcgtcgcaa agccttcgtc
     8041 cgcgcccggc ggctcgtact cgcggcgata gcggaattgc gtggtcggcg cgatcgtctg
     8101 cgtcttgcgc agctcggcgg gttcgagcaa gcggccgtgc aactcgagca tgcgcgcgat
     8161 cgcgttcgcc tgtgcttgct cgagcgaggt ggtcgtcacg aggcagcgca cggcgacgtc
     8221 atgacgtttc gcaacttcga caaccggagc acgcagtgcg cgcgtgccgt acgtgttgtc
     8281 gagcacgacg tggttggcgc caccggccag ctcgcgatcg agcacgcgcg ccagctcgag
     8341 caacgtgccg ccgcgctcgt cgcgattgag tcggacgtaa ccgcgcgccg cgtagtcgag
     8401 ggcgagcgtc gtcttgccgg ccgccggcat gcccatgatg atcaggacct cgcgttcgcc
     8461 ggcgtgtgcc gcagcgcgcg gacgctcggc aattgcgacg aagtgggcgt cgagcgcggt
     8521 cacggcgtcg gcgtcgagct cggcgacgtg cgcggcactt cgcgcggtct cgatgcgggt
     8581 ggcgccgggc agcggcacgg taccgagctg gcgcagccac gcgagcacga tctcgtacgg
     8641 ggtcgcgccg aggcgggttg cgatctgagc gagcacctcg tcgcgcgcga tgcgcttcac
     8701 gcccgccggc ccgccgaatg gccgatgtgc gagcacgcga atgttgcgct gcttgcaggc
     8761 cgcgagcagg ccgccgcgca gtggatcgag gtgccatggt gacagctcga cctcgacggc
     8821 gtcgagcggc gcgatcgcga gcgcttgctc gagctggtgc acgccgacgt tcgacaggcc
     8881 gatcgcgcgc acgattccgt cttcgcgcaa cttcgcgagt gcgcgcacgc tcgtcgtgag
     8941 cggtacctgc ggatcgacgg cgtggagcag gtacagatcg atcgcgccga ggcgctcctt
     9001 gctcgcacgt gcggcggcgg cgaggtgacg cgcgcggcca ttcggtaccc atgtaccgtc
     9061 ggggcgcacc aggccgccct tcgtcacgac gtgcacgctg cgaccggcga tcgctcgcgc
     9121 gatcagctgc tcgttgtggc cgacgtcgcg atcgtcctgc gcgtacgcat cggcggtgtc
     9181 gaggagatcg acgcccgcgt cgagcgccgc gctcaacacc gcgagcgagc gtgcgtcgtc
     9241 gcggtccttc gccgtggaca gacgcatgca gcccagcccg atcaccgctg cagtataggc
     9301 gccttgtccg tcgcgacgac cgtacgagtt gctcgcggcg acgtcgccgg cttctgggcg
     9361 agtcgcttcg ccgcgacgcc caggctgtgc gcaccgttca cgccgagcgc ggtcactgcg
     9421 gtctcgagcc gaccgcgcac ttgaccgcgc gcgaggatga tctcgccatt cggctggcgc
     9481 tggatctcga tgttgggctg ctgccgcatc cacgtgtagt cgatgtccat cgcgccctgg
     9541 accatcgcga tctcttcgat cgtgaggccc gactccgcca tccaatcgaa gaccggtccg
     9601 tccttgacgt cggcgagccg catcttgttg tcgatcgcgg cgatttcgtc gacgagatcc
     9661 tcgtgacccg agcgcgcgat cagctcggcc atcgggcacc gcacaccgcg tgcgtccttg
     9721 aagacgctga tcgggcggcc tccctcgtcg gtcgggtata cgcctgcttc gtagtagtcg
     9781 cgcagccagc tgatcatctg cgcacgcctc gcactcagct cggcaatctg cgtcgctgga
     9841 gcattcgtcg tcgaccgggg cgccgtctgg caggcgcata ctgcgagtac caagatccat
     9901 ttcgtcatcg tgtcctccga tgcagccacg atggctcgcg caaccacgtc acggcgtaag
     9961 acctctgtaa gcgcacttct gctcgcgatg ttgatcgctc actacgccgc ccactgacgg
    10021 ctgtcactcc ggagcggttt ccgttgcccg gccgcgagca ccgtggttag ctgggctcaa
    10081 atgggtcggt cgctccgctt attggtcgtc gaggaccacc tcgattcggc cgaactgctt
    10141 gccgagttgc tcgccaataa gggccacacg gtacgcgtag cgacgaccgc atccgacgcg
    10201 atcgcgatcg caagtgaaca gccgttcgac atcgtcgtca gcgacgtcgg tctccccgac
    10261 gcgaccggct acgagctgat gcagcagctg cgcgagcgct acgagatcaa gggcatcgcg
    10321 atgaccggct cggggcgccc ctcggatatc gcggcgggca aggctgcggg attctctgtc
    10381 cacctcacga agccggtctc gatgcaccgg ctgcagcagg cgctcgaaga gctgggcggt
    10441 tagctcgact gtcagccctg gtcgttaccg tgacggccat gatgacgttc aacggtgatg
    10501 actcgccgcg cgtcggcgac cgcgtgttca tgggcaacga gctgctcgcc gagattgcgc
    10561 gtcaggcgcc ggggctaccg ccgatgtggt gctacctgtc ggcaggcacc accggccggc
    10621 tgctcggctg gcgcggcgaa acgcgcgcga tcgttgatat cgacgatgtc gatcgccggc
    10681 tcgtcgtcta tgtgtctgcg ggccaggtcg cgcgcgcggg gatggcatca cgagctgcgt
    10741 aaggctcgtg taaggggcgc ggccttcggg gaaacgccct gcaactgcgc ccgcgttcgc
    10801 tagtctttct aggcatgagg cttccgcccg tgctcatacc cctcgtcgcc tgtgcggcct
    10861 gttcggcgcc ggccagctcc gaaccgcgca atcatcaagc gaaggcgcag gcgccgtcgt
    10921 cgccctacgc cgatcacatc gcgaagctgc gcaagcgact cgcgaagctc ggtatgcgcg
    10981 atgtcgagat tcgcgtcgag gatccgttcg tggtggtcgg cgacggcacg gcggaggatc
    11041 tggtgcacga cgccagcacg gtgcgatggg cggtcgagca cctcgaggcc gacttcttca
    11101 ccgcacggcc cacacgtgtc ctcgacgtct atctgtttcg cgacgccgat agctacgagg
    11161 ctggcgtcaa gcggctgacc ggcgatgcgc cgagcacgcc gtacggcttc tactcggcag
    11221 caaacaatgg cctgttcatg aacatcggca ccggcggcgg cacgttggtc cacgagatcg
    11281 tgcatccgta cgtcgaggcc gattttccag acgcaccggc gtggctcaac gagggtctcg
    11341 gctcgctgtt cgagcagagc gcgacgacga gcgacggtca catcatcggg ctcacgaact
    11401 ggcggctcgg cgggctcaag cgcgccatcg ccgagaaccg tgtaccgacg ttcaaggcgc
    11461 tcacgcatct cgccgacgat gcgttctatg ccgacgacac tggcgtcaat tacgcggcgt
    11521 cgcgctactt gctctattac ctgcaggagc agggcaagct gcgcgagttc tactctgcgt
    11581 tccgcgccgc gcgcgcgaag gatccatccg gctacgcgac gctcgtcgcg acgctcggcg
    11641 cacccgacat ggcgaagttc gaggcgagct ggcgacagta cgtcggcaag ctcagtttcc
    11701 cgtgacgacg cgatcgctgc gatagatcgg tagcagattg taccgttcat cccacgccgg
    11761 gtgcttccgg tagaaccact cgcgtttccg ctcgggactc tttgcgagct cgggatcggc
    11821 ggcgaccgcg gcatcgaact gcgcgcgcag ctgcggatcg cgctcgagca tctgccgcgc
    11881 ctgctcctcg acgacgtacg cctccatgta ctccttgcgc tcgaatgcgt tcgtcaagaa
    11941 gccccactgc gcgaacgaat ccgggccgcc gggatcgagg aggtggacga tcagctgcac
    12001 gcgcgactga tcgagcggca cgaagatcgc gccctgatcg agcgtgcgcg tctccttcgc
    12061 ccacgcacct tcgagcgtca cccgcgtgcg gccctcgaac ggcttctcga atgtcacttt
    12121 ggtcgcgcgg tacgcgtcca catcgacgcg cggctgcccg gcgatcttcg tgtagcggat
    12181 gccgtgatag tcgagcacgg gcgcgagttg cgctgcgaat ccgccgtcga tgatgtagcc
    12241 cgcatgcggc acgtgcgcga tgacctttgg cttgacctcg tccttgagcg gcaccttcca
    12301 gatctgcggc ttcgcttcgt cgtagacgag ccacgtaccg ccggtcagat cggacgtctt
    12361 gcgctcgaac gcgtacccgc gaaaatcgat ctgcgtgacg tggtccgtgt tctcgtatac
    12421 cagcggtagc tcgccaccgc gcagcgactg gtcggccgcg tctgcatcgt ttgctgcctt
    12481 cacccagacc ggcgtgtcct tcagcgcttg ctcgaacagc gcctgcagca ggtgatacgt
    12541 gctctgcacg cgctccttgt acgtgcgcca gctgtgcgtc tcgacgagaa tgccgatccg
    12601 cgagcgggtt gcggcgtact cctgcgagaa gcgtggtggc gcgtcgccgg ccgcaaaacc
    12661 cgacatcgga tcgtcgtcct tctcgaatga cggatagaac ggcagtggca ggtgcccgag
    12721 cgcggtcatc cgtgtctgca gctgatcgga tagcgcgtgc gcagtctcgt cgagatgatc
    12781 ggcgcgcgcg tacggtgcga ccagcacggc gatgtcttgc tcgaacttcg cgccgtcggt
    12841 cgcgtgcaga tcgacgaaca cgatcggatc gaaacgcttg aacacgccga gcagcgcttg
    12901 cacctcgcgg gtgtcggctt tgacgaagtc gcggttgatg ttgttgcgcg cgccattcgt
    12961 gcgaaagccc atctctaccg gcccgcgctg attgggtcgg ttgttcgccg agaatcgctc
    13021 gtgaccatcc gggttgatca cggggatgaa gccgacgtcg accacgtcga gcacacctgg
    13081 cgcgaccctg ccgtcgagca gatcgcgcat gaaccaaaac ccagcgtcct tgccttcgat
    13141 ctcaccggcg tggatgccgc cttcgaacaa gatcatcggc cggcctttgc cgcgcgacac
    13201 gcgcaacatc acgatcggcc ggtcctcgag cgtgcggccg atctcctcac agcggacgcc
    13261 ggcgtagacg cgtgcgaagt cgtggcagag cgtgacggct tcgtcgtagc ggccggtctt
    13321 cacgaactgc gtcgcctcgc cggtcgtgac gaggggaagc cttggccgcg gcgcgtggca
    13381 cgcgacaacg acaacaacaa gcacgagcag gcgccacatc gcgggcgact gtagcgaagg
    13441 acaggcccga gagggccgga accggcgcta ctcggaccgc ggcggacggg gcgcgtgggc
    13501 ccagttgtcg acgatctcgt cgtcgccgca gcgcgacaag tagtcgaggc cgaacgaatt
    13561 tgcttcgatc gtgcggccgt ccatcgcgag tagcttcgcg agctcgcgcg cacgctcgag
    13621 atcgcagcgc tggccgagct cgacgcgcac gatctcgatc cggagcttgc gggtctgcgc
    13681 gatctccgac agctcgcggc gcatcagctc gtgacggctg accttgcgct ccggaatgat
    13741 cgagatggcc cagccggtga tcgcgaacac gaacagcacg atcatcgaca gcacgatcgc
    13801 gcgctggccc ttggcgcggg caagcggctt gtcgagcagg tgcgtcgtgc gctcgttgat
    13861 cgcgttccag tcggcgcccg gcacggcgag cttgagatct tcgatcgcgc ggcgcgcgtc
    13921 gtcgatcgct gccttgtcga tgtcggtgcc ttctgcgttc gtcggcgcgc ggcgcgcatt
    13981 cggccgcatc tcgtcgtagc gggcggtgtt ggcaccgcgg gtcgcgagca cgctgcgcgc
    14041 gcgatcgaga tggcgccaga tctccggata cgtgtcgtga acgtccgtga tcgttcgcca
    14101 ccgttcgttg tgcgacagcg tctcgctcgt ccggatcagc gtcgctgcgc gctggcactc
    14161 gtcgacgagg gcttcgccga tgaggatcgc gccgaccgcg cggtcgcgtt catcgagggc
    14221 gttcgcggag tcgcggtaca tcacgatcag tgtttcggat cggagccaac taggcaagac
    14281 ggaaacggat aacgccgggc tcgccgcttc tggcagcaag cggtaactgc ttcaaaagtc
    14341 acactcaggc acgctgtccc aggcgtccca ccgtcgagcc gtccaccgcc gcaggccgcc
    14401 gcggatcaca accgcagcga atggcaggct ccgatcaggc agccgaccgc gacgtcgaca
    14461 gcggtcaccg cggcggcggt gccgagcgat gccccgatcg tgaagtcctc gacctgcgag
    14521 gcgattatcg tcgtcacgac caggtctgcg gccacgccgc cgacgagcat cggcagcgag
    14581 acgttatctc gcttccagat tccggcgccg gtcacgcagc ccgccaaggc cagggtcatc
    14641 gcgacggctg ctgcacgctg catgatgtga gtgtaacgcg ggaagaatgc gccctgttca
    14701 ggcgtctctt cgttgtggct gccacgcagg gtggggccac atgaggaggg tcgcgatgga
    14761 cactctcgcg atcgaattca caggaacctt cgagctagcg ctctggtgca agttgcttcg
    14821 cgaccgcggc tggaccgggc atcaagtcgc attctcgctc ggccgcagcg aaggctatgt
    14881 caacaacctg atccgcatcc tcgagcgcgc gtcgctcgag gtgctcgccc ggtggaagaa
    14941 cgagcaggca acaggcgccg gcctggtgcc gatctgcgcg accgactggc tgatgcaggt
    15001 ctgcgtgctg ccgcacgacc agcagctcgg cgagctggcc agacgcgtcg agcgcttcga
    15061 aggccgcaag cgcgaacccg gcgcatagcg aagcccgcga gacgaatgcc tcgcgggctc
    15121 ctggggcgca gagacgagcg ctacgcgatt acttgtcgcg cttgtccttc ttgtccttct
    15181 tctcgtcgtt cttcttcgtg cggtcgtcgg tcttgtcctt ccccggcggc gtcgcgcgat
    15241 ccgcacggtc cgacggcgtc gcgcgatccg cacggtccga cggcgtcggg tgctcgttcg
    15301 cgttacccgc aggtggcggc gacatgccgt gatcgtgctc gatgcccgga ttgcccggcg
    15361 tcgcgggtgg cgtcgtgtga tcgatatccg gcggcacgtt gcgctgcggc tgcggctcgc
    15421 gacggttgtc gttcggcgtt cgcgccggct cacccgggtt cggcggctcg gtcggctgcg
    15481 tcggattcgc tcccggtggc tgcgcacccg gcacgttcgg ctgatgtggc tgatagacgg
    15541 ggctcgccgg tgacgtccgt ggctgctcgt tcggcgttgg atagccgggc tgcgctggcg
    15601 cgggcggcgg atacgtcggc tgtgcttgcg gctgcgccgg cgtttgatac gtcggcagcg
    15661 aacgcgttcg catgtcaggc gtgcgcgccg cgcggtcgcg acccatgtgg ctgcgatagt
    15721 gggcgtaggc ctccggtcgc gagatcgttc tgatccgctg cggcacgtac gagaactcga
    15781 cgcgcacgta attgcggcgg tagtcgtttg atcggaacca gtagccgtcg cgatagagcc
    15841 agtagtcgtt gtcggcgtag aagatcggtt catcggcatc agcgatgacc tgaacgccgg
    15901 gactgattgc gatcagctcg ggtgaggcca cctgcacttc gccggcgtat tcgacatcgc
    15961 ccgtgcccgt acaagcagcg agcgatgacg ccacgacgaa gatgcatgcg gaaatgcgtg
    16021 ttctcgtcat ttacgtacct ccgccgtgac gatcagcatc tcccgtgcca cggggaaaaa
    16081 ctcgtatgat tcgcgaatga gcgaagtcga cgacgggaaa gaagacggca agaagaagcg
    16141 gaagctgcga acgctcaccg ggatctccgt cgacgagttt cgacatccac tcgatcaacg
    16201 cgcgacggag gcactgaaga aggtgccggg tctcgacaag ctgatcgcga agatcctcga
    16261 aatcggtctc gagcgcatgc tctacatcaa gaacatcggc tcgaacctgc gcgtgacgcc
    16321 caagatgttt ccgcgcctgc acaagagcct gacgtgggcg acgaagatcc tcgacgtgcc
    16381 cgagcccgag atgtacgtga caaccgatcc gcgcccgaac gcgttcacgt tcggtcacac
    16441 gaagccgttc atcaccctca ctaccggttt gatcgacatg atgaccgacg aagaggtgtt
    16501 ctttgtgatc ggtcacgagg tcggccacat caaaggcggc cacgtgctct acatgacgat
    16561 ggcgtacaac atcgccgcaa tcctggagtt ggtcggccag ttcacgctcg ggattggcgc
    16621 gctggtcgga cgcggcctcg agtacgcgtt actcgagtgg gtccggtgcg ccgagctgac
    16681 gagcgatcgg gcggcactgc tgtgcgtgca ggatctcgaa ccggcgcgct cgacgttcat
    16741 gaagctcgcg ggcggcacga cgcggctcgc cgccgagatc gatcgcgacg agttcttgcg
    16801 ccagattcgc gaatacgagg acgtcgatca ttcgacgctc gatcgcgcgt acaagctgct
    16861 gttgaccatc gatcgtacgc atccgttcgc gatgcagcgc gcgaaggaga tggagacctg
    16921 gtacgagagc ggctacgccg acctgatggc gcggcatccg tagtgcaacc cgcgggactc
    16981 gcttcgcggc gaggtcgcgc gagcagttgc gcgagcttgc gtgtggcacg cgcgctgctc
    17041 tagcgcaggc tcatgcgcaa caccgattcg atcgctagtc tcgatctcga gcttctaacc
    17101 accgtcaccg gcggctgcgg cagcaagaaa cagcagcaat gcgcgccgcc ccaagcacca
    17161 ccaccgcagc agagcgaggg cggcggcacg aacgtcgtcg tcgcgacggg cacgcaaggc
    17221 ggcgcgatga tccagcaagc gttgcagggc ggcgcgatgc cgggtgcgcc gcaaccgccg
    17281 cgcatgatct gagagagcgg cgcacgtgcg tccggtggcc tatcgcttgc tgcgttgggc
    17341 cacatgaggg atcgcatcat cgctaacgac tcgggctcga cgcgttccac ctggatgaca
    17401 gagtgggttc cagacttcag tgcggagatc gctgagggtg agcaccccga ggtctgcgtc
    17461 atcggcgctg ggatcgcagg gctcagcgtc gcagtcgcgc tcgttcgtga aggcgtcgat
    17521 gtactcgtgc tcgaccaggg tccgatcggc ggcggacaga ccgcacgcac gagcgcacac
    17581 gtcgcgtctg cgctcgacga ttacttccac gtcctcgagc gccggttcgg ccgcgacggt
    17641 gcgaagctct gctacgagag ccacgctgct gcgatcgacg agatcgagaa caatgcgcgc
    17701 cggttcggca tcgattgcga cttccgccgc gtcgatggct atctgtgggc aggtagcgac
    17761 cagcaccacg cgatgctcga gaaagagctc gccgccgcca agcgcgtcgg cctcggtgtc
    17821 gacgagatcg cgcgcgcgcc actgccgttc gacaccggtc cgtgtctacg gttcgcgcgg
    17881 caagccgagt ttcatccgct caagtatttg cgcggcatcg cgcaagcgat catcgcgggc
    17941 agcggtcgga ttcacaccgg cgtgcacgtg gtcggcgtga agagcggcac gccggtcgag
    18001 atcacgctgc agggcggccg cacgctgcgc gcgaccgcgg tcgtcgacgc gacgcagatg
    18061 gcgatcacca gtcgctttaa catgccgctg cgcgaggccg catatcgcac gtacatcgtc
    18121 gcgctcgcga tcccgcacga ctacgtgcca cacggcctct actgggacac gctcgatccg
    18181 tatcactacg tgcgcgtcgc tgccgacggc gacggccgcg agatcctgat cgtcggcggc
    18241 gaagatcatc gcgtcggcca gggcgatccg gagattcact tcccccggct cgaagcgtgg
    18301 gcgcgcgatc acttccccga cgcggggccc gcggtcgcta cgtggtcggg acaagtgcag
    18361 gagccgagcg acggcatggc gtacatcggc aagctgcccg gtcacccgca catctacgtc
    18421 gtcaccggcg actcgggcaa cggcatcacg cacggcaccg tcgccggcct gctcttgccg
    18481 tcgctgatcc gcgacaggcc gcatccctgg cagcatatct acgcgccgaa tcgatcgcgg
    18541 ctacgcggcg ccggcgagct cgtcaaagag gcgatgcact cgaacgggcc gtacgtcgac
    18601 tggatgcgcg gcggcgacgt ctcgaacctc gaggacattc gtcccggtca tggcgcgacg
    18661 attcgcagag gtctgcacgt gatcgccgcc tacaaggacg agcgcggtca gtgtcacctg
    18721 atgaacgcgc gctgcccgca cctgagcggc gtcgtgcgct ggaacgaagt cgagaagacg
    18781 tgggactgcc ccgtgcacgg ctcgcgtttc gattgcgaag gacgcgtgct gaacggtccg
    18841 gcatcggtcg atctcggcga ggcgacggcg gatatcgagg cgccgatcca gatccccgag
    18901 ccggtgttcg gcgaggaacc gttcacgctg aagccagtct gatcaggctg cgcggcgttt
    18961 caggacatcg cgcagcggcc gccgatctca tcagacggcg cggcatttca ggacatcgcg
    19021 cagcgagcgc cgatctcatc aggcagcgcg gcatttcagg acatcgcgca gcgagcgccg
    19081 atctcgtcag gcagcgcggc gtttcagaac atcgcgccag cgcacgcgac gcggcggtcg
    19141 cgcagcagtg ccgtccttcg cggcccgtgc gcgttcgcga ttgcgcgtgc gatcggcgag
    19201 ctccttcggc gtgccgacat attcctcgcg cgtgccgcgc agtacgtcga accacttgaa
    19261 cgtgacgccc cagttcttgt gctggttcgg tcccatgtga tggtcgtagt gccacgccag
    19321 gtgcgtgcgt ccccactcgg gatcgatgtg gcagcgccgg tgatcgcggc ggtacttcgc
    19381 gagcaggtac cacgtggtcg cggtgtagaa cggcgcgatc ggaaacagcg gcgcgtgcgc
    19441 gaggccgatc acggtcaacc cgatcgcttc accgagctgc gtcggcgtgc tccacaccgg
    19501 tccctcgaag tccggatcgt agccgccgtg tctgcgcacc ttctgatggt gcacgtgaaa
    19561 gtgaaatgcg aagacgctgt ccttgttgcg gccgcgccag tgcaacaggt agcggtgcgc
    19621 ggcccattcg ccgtacgcaa agctggcgat cgcgagcggg atcccgatca tgggggaacg
    19681 atcggacccc gctcgcgacg cagcaagtaa caatctcgag tcagcggcta gactcagccg
    19741 cgttgattca ctcctcgacc gtgccggccg cgagcgagag atccatcttg gtcatctcga
    19801 gcaggccggc ccagttccac agctcaccca tcgcctgcag cgacagtgac gcgtcgatgt
    19861 gatcgcccgc gcccgtcagc atgacgtcga tcgagaccgg tggcaggtgc tgcgtagcga
    19921 gcggcgtgtt gctgccgttg atgtgaatcg accagtcgaa gtcgagcatg agcggcagcg
    19981 tcagcgtcgc gtcgttgtcg tcagtccatg cgacgtccgc atgcgcaggc gcggtcagcg
    20041 acacgcgcac gtcgtcgagc gtcgcgggct tcttgaacac gtcctccggg atctcgatcg
    20101 gcgcgaggct cacggtgagc cggttgaccg tcaacgcgcc gtgcgagtcg acctgcgcca
    20161 acacttcacc gccgtcgatc gtcacctgcg tcacgccctc ggcccagcca tcgctggtct
    20221 tgcgcttcgc agtgatgctg ccggtgctgc cgtccgcgcc cacgtagagc cgggtcgggc
    20281 cgtcagcgag ccgatcgcga accgaatcga aatgctcccc gcccgccgga tcgttcgaga
    20341 tgcacgccgc cacaaacaga gtcaaagcca ataacgtacg catagtaacg caacgcgttg
    20401 caatgtccga tccaatggtc cgaccatggt ccgaccattc tgtatcggct aacgattcca
    20461 gctatcaacc ttcgttcaac agccgttcaa agtgcgccac tttggcgcaa tccggctggt
    20521 agacgagccg tcgacgcgac gcatcaatct gttagcaaaa ggggtctgac cggtctgacc
    20581 ccatttctgg cgtggctacg gctgaccgaa atcgagcgcg cccttgggct tctcgggttc
    20641 ggtcttcgcg ccagcgggct gctgctttgc gccgcccggc ttcgcgtcgg cgtcgtcttg
    20701 cttgggctgg ttctttgctg ccgcttcgga cgcgtcgagc cgatcgagcg cgcctgcaga
    20761 gacgttctct ttcactttga ccggcttctt ttcctcgttc tgtcgcttct ccggcgtgtg
    20821 ggaatccgga gacgcgagcg tgattgcggc agctgctgcg gcagctgcac ccgcaaccgc
    20881 acctggatcc ggcttaggtg cattcgcgag taccggctga ttcggctgtg cgccgcatcc
    20941 agcaaggagc acgaacacga tcgctcgcat gatcggatgg tacacctcgc ttcgccatgc
    21001 gtgccatcgc gatcacgaag cccggcggcc cggaagttct tgcgctcgtc gagaaaccac
    21061 agcccgagcc cgcgcgcggc gaagtgcgcg tacgcgtgcg tgcgaccgcg gtcaaccgcg
    21121 cggatctgtt gcaacgcatg ggtggctacc ccgcgcccag cgattcgccg cccgacattc
    21181 ctggcctcga gatcgccggc gaggtcgaag cgctcgggcc aggcagcgca cgatttgccg
    21241 tcggagatcg ggtgttcggc ctggtcggtg gcggcggtta cgccgaacag atcgtgagcc
    21301 acgagcgcgc gctcgccaag attcccgacg gcatgagctt cgaagatgcg ggcgccgtcg
    21361 tcgaagcatt cgtcaccgcg cacgacgcga tggtgacgca agcgcaactg aagagcggcg
    21421 agccgttgct gatccatgcg gtcggcagtg gcgttggcac cgccgcggcg cagctcgggc
    21481 gtgcactcgg cgcattcgtg atcggcaccg cgcgcacgca agacaagctc gatcgcgcac
    21541 gcgcgctcgg catggacgcg ggtgtgatcg ccctaggcgg caagtttgcc gacgccgtcc
    21601 ggcgcgttca gccgcaaggc gccgcggtga tcctcgagct cgtcggcggc ggttacctcg
    21661 ccgaagatct gcgctgcgtg cagacgctcg gccggatcgc gctcgtcggt ctcatggccg
    21721 gcgcgaagac cgagctcgat ctcggcctcg tgctcacgaa gcgcgtgcgc attctcggca
    21781 ccgtgcttcg cgcgcgtccg ctcgaggaga agatcgccgc gatgcgtgcg ttcgagacgc
    21841 aggtcgtacc gctgctcgcg cgcggcgtcg tcaaaccggt gatcgattgt gtgatgccgc
    21901 tcgccgacgc cgcgaaagcg cacgagcgca tggcgagcaa cgcgggcttc ggcaagatcg
    21961 ttctgcgcgt cgattgatca gcgctcgcag acgcgtgtcg tgatcacttc gcacacgcgc
    22021 agccgccgac ttgatgacac gtgtctgtct cctcgatgca cgtgtcaccg cacggcttgc
    22081 cggtcgtgca gaccttgcag cagttgctgc gttcgctctg cttgcactct tgctcgcagc
    22141 tcgccgcgaa catcatcacg aggacgaacg accagcgcct gtgcatgtct ccattatatg
    22201 acgccggtca tgtccatgac ctcgagaacg agttgcgcgg aaccctcgta gcgcggtgcc
    22261 tcaccgccga acatcacacc gcccgtgagc tgcagcgcca caccatgcag gcctaacgtg
    22321 aggcgagccg tcggaccgac gccacgtagg ccggtcagct ccgcttcggc accgagatcg
    22381 agaccggcgg agccgacccg gttttgatac cagccgtacg ttgccgcgac ggccgtcgtc
    22441 tcgcctgcat cggtgcgcgt gtcgagcagc acgcgcgtca cgctcgagcc cgggaacaac
    22501 cagtctgtct gcttctcgcc ccaggcgtaa ccaatcgcga agctgccgac gctgccttcg
    22561 gtgctcgagc caccaccgac gatcacgatc ggggtgtgca cttgcgtcgt ctcgaccttg
    22621 aacacgtcgg cgagcatcgc gccgaagtcg ggcacgccgc acgcggcggc gggacgcgca
    22681 aatgcacttg cagcgatcgt gacggcggcg acggcgagca gcttcatgca cctgtagtac
    22741 gcacgacgaa tcgcgattca cggggcgggc gcgtgatctt cacgtgaagc gcggtgcgca
    22801 actccgcgaa tcgcggcccc gagcggcaga ttgcgaacct ctcggcacca ctcagctgac
    22861 ccaacgcgtc gaagatcttg tcggaataac gtgttatgtc gacatggtgc accctgccag
    22921 cgcacggctg atcgagacca ttcgcgcgtc gatcattggc gatgatcacg cgctcgaagg
    22981 accctacggc ccgcgtcgcg tgacgtacgc cgactacacc gcatcgggac gctcgatcac
    23041 gttcatcgaa gagttcatcc gcgaacaggt gatgccgctc tacgcgaaca cgcacaccga
    23101 gacgagcggc accggtgcgc agaccacgca gtttcgtgaa gacgcgcgcg aggtcattcg
    23161 ccgcgctgtc ggtggcaacg acgacgattg cgtcatcttc tgcggctcgg gcgcgaccgg
    23221 cgcgatcaac aagctgatcg acatcctcaa cctgcgcgtg ccgaacgatc tcgacgcgcg
    23281 ctacgagctc acgtcgcgca ttccggaagc cgagcgcccg gtcgtgttca tcggtccgtt
    23341 cgagcaccac tccaacgagc tgccgtggcg cgaatcgatc gccgacgtcg tcgtgatcca
    23401 cgaggacggc gacggccacg tcgatctcgc gcacctcgag caagagctcg tgcgctacgc
    23461 aggccgcagc ctcaagatcg ggtcgttctc cgccgcgtcg aatgtcaccg gcatcgccac
    23521 cgacacgcgc gcgatctcga cgctgctcca ccagcacaac gcgctgtcgt tctgggacta
    23581 cgccgccgcc gggccgtacg tccagatcag catgcacgag gacgacacgc tcgcgtacaa
    23641 ggacgcggtc ttcatctcac cgcacaagtt tatcggcgga ccgcagacgc cgggcgtgct
    23701 cgtcgtcaag cgcaagctgc tcgtcaacac ggtgcctgcg tgtccgggcg gcggcacggt
    23761 cgcatacgtc aatcaagaag agcaccgcta cgtcaccgat ccggtgattc gcgaagaagg
    23821 tggcacgccc gcgatcatcg agtcggtacg cgccggcctc gtgttccagc tcaaggaagc
    23881 cgtcggtgcc gacgcgattc gcgaacgcga ggtcgatttc atccagcgcg cgatcgcatc
    23941 gtggtcggcg aacccgaaca tcgcgatcct cggcaacaag agcgcgtggc gactctcgat
    24001 cgtctcgttc atcgtgcgcc atcgcgaggc gtacctgcac cacaacttca tcgtcgcgct
    24061 gctcaacgat ctgttcggca tccagtcgcg cggtggttgc gggtgcgccg gaccgtacgg
    24121 tcatcgcctg ctcggcatcg acgtcgagac aagcaaggag ttcgagcgcg agatcgtgcg
    24181 cggctgcgaa gggatcaagc cgggctgggt acgcgtcaac ttcaactact tcctgtccga
    24241 gacgcagttc cagttcctgc tcgacgcgat tcatctcgtc gcgaacgacg gctggcgcct
    24301 cctcccgcac tacacgttcg atccggacac cggcgagtgg cgccatcgcg cgcactcgag
    24361 gaagcatgtg atgagcctcg ccgaggtctc gtatcgcacg ggcaagatgg agtaccgctc
    24421 gcggcactcg accgagcccg agtgggcgct gccgaattac atcgacgaag cgaagaagat
    24481 catcagcgcg accgccgacg agttccgcac gttgcagatc aaggaagccg agcgcagcga
    24541 agacttcgag gagctgcgct ggttcccgct gccgagcgaa gtgctcgaag agctacgcgg
    24601 tcaaccgacc ggtcgccacg cggcgctgct gcggccgtaa tcggccgcga gcggtcaggt
    24661 tgctcagtcg ccgccgccgc tgcgcgacac aaagcgcaca gcgaacagaa gctgtacgtc
    24721 gtaggccgtg taatcgagcg ggatgaaatc gcccggattg tcgtcgtcat gcgtgctgat
    24781 cgggatcgca agctcgccgc cgacctcgag cccaccgacc ttcgcccata ggccaccagc
    24841 aaactcgaag ccgaggccaa cgtcagtatc ggactgatcg ccgacaaccg agtcgatatt
    24901 cacgtatgcg atgtcagcgc cgatgccacc acgcgctgag aacgcgagca ggttgctgat
    24961 gttgtggtgg ataatgccgc ctgccatcaa gcggaagcga tgtgcagaga catctgcgaa
    25021 ctgattgttc tcgtagtcgg tattcatcgg cgcccactca gcgccgaaga agagtcccag
    25081 ttctttcgga aacgcaccgg ctcggagggc taggacgggg ctcgtttcca cgttgttcgt
    25141 gtagtcgtca tcgcctgccg gaatcgaaat gccgccagcc agctcgacgt agctgtcggc
    25201 gtgggcgacc acgggagcac agagcaaggc cagggcaata atggctctca tccccctgag
    25261 ggtaacgctg tcatccgcgg gactcgcaat gtcacctggc ggtagacaac gttcatgtcc
    25321 ggatcccggg cgatctcgcc gggataggca tggcacacgc cgtgctcaat gcaccagcac
    25381 gaaggagcat cccaccatga aaaccatttc ggaagttgaa ctggcaaacg tgaccggcgg
    25441 caaagttccc gacgcgacct ccgtcacgcc cggcagcagc agcagctcga acaatgacgc
    25501 ggtgatgtcg gcgctcaatg gcatccagtc gtcgctgaag gacctgcaaa acccgcaggg
    25561 cctgtttggc ggcaacaacg gcctcttgtt catgacgatg gcgttcgcga tgagccggcg
    25621 cagtgaagtc gtcgtctacg gcggtggccc gcgtcgcggc tacagctggc gcgcctactg
    25681 gtgaacgcgc agctcatgcg cggcgaatcg cggccacgat gaggccgtga aactgttcgc
    25741 cggcattgcg atagatccag ccgacgtgcg cgcgacagtt gccgcacgcg gcgacctgcc
    25801 acgtccatcc cggaaaccac gagaacgcac tctccgtcgg gccgagatag aggcagccag
    25861 gcgccgcgtt gaagcagccg atgtggtgca cgaagccgcc cggattcacg aacgtatgct
    25921 cgtgcgcgcc ggccatctcg atgcggtgat cgtcgtcgga gatgcgatgg ccgcacacca
    25981 cgcacacgag tgcatgttcg tcgcggccta cccggtcgtt caccgacttc ggtgacgatc
    26041 tgcggtccct ctcgcgatcg agcagcacga accgagtgta gaatgactgc tccatgtcga
    26101 tggccgccga tcgcatcctg ccgctgtgtg agttgttgct gggcgccgca tacgcggaca
    26161 aggagctcaa ggatcaagag aaggacgaag tccgctctct cctcgaggga cttgccggcg
    26221 agctgccgac cgaagtcgag atgaagatcg cgagcttcga tcccggctcg ttcaagctcg
    26281 agacctccgc cgccgtgttc aaggcggaca gcgaagacga tcgcaagaag ctcctctacc
    26341 tcgtcgccgc tgtgaacgaa gccgacgaag agatcgactt cgccgaagac gaatacctgc
    26401 gtgacctcgc gagcgcgctc gccctaccga agtccgcgct cgatggcctc acggtcgaga
    26461 tcgagaccga agagctcaag gaaacgttca agcaggtccg caagggcccg ccgccgccac
    26521 cgccgagcgc gaagagcaag tccgtcgaca tcgacatgga ctgagcgcct cgcgcgcttc
    26581 actctgcgag cgctctcgcg gccttccgcg atcgagcttg acgatccgca gcgcgctgat
    26641 caaaatggcc gcgcttacgc ggaggtttgt catgttgctc cttgaaccct cccgcgccgt
    26701 cgccgagtaa tcgcgaccgc gcggacattc actcatccga acggctggat tcatccggcg
    26761 tggcatcgcc atgcccgagg agcaacatgc acaaccgcaa agacttgcag ctgtgtcgac
    26821 aggtgtacga cgcactgacc tacgcgctcg ccgacatcga ggatctgatg atcctcgatc
    26881 tcgtgctcgc gtcggtcgtg ccggcaccga gcgcggcacg cttgcaggtg acgctggtgc
    26941 ctgctcacga caacgtcgac cccgaagaag cgctcgcgcg cgtcaaagag gtcgccgacg
    27001 aattgcgtga agaggtcgcc gccgaagtca cccgcaagcg cgcacccgag ctcgtgttcc
    27061 gcatcggtca ccgcgacgag ctgtaaccgc caaggcggcc gagatccgtt gccgggtctc
    27121 ggccgtcttc tcttttcaac cgccaagacg ccgcagacgc caaggcagaa tcaattatcc
    27181 ggagaagatg actcgacgga tcccctggcg aagcagcggg acgttgaagt tgatgagcag
    27241 gccgagctga aacgcgccgg ctctcaaata cgaaagcact tgtgccatgt ggattggtgc
    27301 aagcgattcg ccggccttga gctcaacgat caagatgtcg tcgaccaaca gatcgacctg
    27361 catgctcggg aggcgatggc ccttgtactc gagctgaagc ggtacctggc gacggacggg
    27421 tattccgcgc agctgcaact ccaccatcat cgctgattcg taaacctgct cgagaaagcc
    27481 tggtccaaga gcgcggtgca cgtcgatcgc agcaccgatc actgtgtttg cacagcgatc
    27541 gagctcttcc gtcggctccg gatgaagaag ttctctgagc ttggcgtctg cggcgtcttg
    27601 gcggttcatt tccggcttgt cggccaagca cgcagcgagt tgcgcactat tcgtcgagat
    27661 acggaaaccg gcttggcggt tccgcccgcg cgagcagctt cgagatgccg agcgcgagca
    27721 cgatcatgaa gaagaactga tcagcgagca gcggcaggcc cggatagtag aggccgcgcg
    27781 ccttccaata gccaggcacc agccagagtg cgaacacgat ggccagcgtg atgccgaccg
    27841 acacgaggcg cacgcgatcg gtgtgcttgt cgcggcggcg gcgatcgatg tcgcgcagta
    27901 gctcgacgtg ccgctcgttc tcgagatcga gcaggccgtc gtagctgcac gtcgcgcagc
    27961 gatcaccgtc ggcttgacgg tgaccgcatt cggggcacgc gaggttcacg cgagcttggc
    28021 gctcatcgtg atcggcgtcg cggcgagggc gcgcgagatc gggcacccct tcttcgcggt
    28081 ctcggcgagc gtctggaact ggtcggccga gacgcccggc accttgccga cgacgtcgag
    28141 gtggatgttc gtgatcgtcc agcccggctc ctgcttctcc atcgagacgg tcgcaaccgt
    28201 cttgagctcg gtcggatcga agttcgcgtt cgtgagctgg aagctgagcg ccatcgtgaa
    28261 gcagctcgcg tgcgcggcgg cgatcagctc ttcggggttc gtacccgcct tgccgtcctc
    28321 ggacgtgaag cgcgtgttga cgctgtacgg ctgctccttg aatgcgccgg agagcgtcga
    28381 gatggtgccg gagcccgtcg ggcccttgcc gtgccagacg gccgtgctgg ttcgcttgag
    28441 tgccatggtg tcctccgcgc gcacgtatac cgcgagtcgc gcgggagtga ctgctcagcg
    28501 tgtccagagc gcagcgatcc cgaagtgaaa cagacgtgag tcttcggtca tgaagttcgc
    28561 ggtcgggctc caggcgccgc caatttcggg gagaacggcc cagcgacggc cgacttgcca
    28621 gcgaaagccg atcgattcgc cgatgaacgg gatgtcgact gggtttgcgc cactcgagta
    28681 gaggcggtga tagccgccgt cgatcgagag cacgagctga tggcggccga gctcgatgcc
    28741 accgatcacg ggaaccgacg cgtagatgtc ttcgatgtgc gcgcccccga ctgccgtgag
    28801 gcgatagccg acgccgctgc cgacggcgag cgaccaccgg ccgcgctcgg cgacgcgcat
    28861 cttgcccgcg agctcgatgc tgccggttga ggcgaccggg gtcggcagga acgtggtgtt
    28921 gacctggacc tcgaagcgtt cgtcgaggcc gcggcgcgcg gcgactgcga gctcgggcag
    28981 cggcgcgaca ccgccgtcgc caccgccttc atcgatgcca acggtgccgg caccggagac
    29041 ttgcgcgccg aagagccact cggtgtgacc gggcggtgcg atacgcgtcg tgcggtacgt
    29101 cgagcctgca ccgcagcccg agacgagcgc ggtcgcggcg gcgaggccga tcgtcgcgag
    29161 caatgcgccg gacactgcgg cggttgccgt ttgtgcgcgc gcggtcacga gcccaccgcc
    29221 gagccaaccg cctcggaata agtgacaacc gcgccgcaca tttcttggtc ggtgccccag
    29281 tggatcgtct tcggtgtctg ctggtggccg ttgacgatct tctggtttgc ttgcgtgttg
    29341 tcccagtgac actcgacgta gagctgatcg cccttcgaga ggacgactgg tgtctcgaag
    29401 tagtagtcgg ccatccagtg gaagtcccag tcggggatgt tgagcaggca ttcggtcgag
    29461 ccgtcggcgc gcaggattgc aacgcgaccg atcgagccga gctcgtgcat gtgcagcatc
    29521 acgttgtgga tgtggatcgg ctcgcctttg gtgagaaccg tcgggtccca ggcgaaccag
    29581 accttggtat cgggcgcgcc ggcaggaatc tccatgccgt cgccgatgaa ccacaacgga
    29641 ttgccggccg cggtgccgcg ctcgacgcgc tcgacatggt cttcgagcat gatgtcgatc
    29701 gagctctgat cggccgcgat gccgtgaccg gtgtcgtaat ggacgttgag caagatgcgc
    29761 gtatgcgcgg ggagcttgcg gccgacaccg tcgggcagca ggcgcggcag cacgccgggt
    29821 tgccagccac cgatgtattg cgcatccgat ccgaattcgc tcgcaccgtc gccccagcag
    29881 tcccagcccg ggcgtccgtc ttcgccgtcg cgcttcgcga gctcggcggc gtgatcttcg
    29941 tcgactgcag cgacgatgat gtgatggacc tcgccgcgca cgccgggctt gaagtcgaag
    30001 ccggtgatgt agcgatcgcg cgggatgtcg tcgtggtcga gcagaaagca gcgaatctcg
    30061 tcggcaccga tcttcggctg cgggatgaac gggacgtgca tcgtcgccgt gagatcgacg
    30121 cgcggcaggc cggttggggg cgcgggcacc ggcatctgat cggcgatatc gccgagcgcc
    30181 atgcccgagc gcagccagcg gagcagcgtg ttgcgttcgg cgtcgctgag cgagcggtcc
    30241 cagcgatagt ggttgcagca atcggccggt tgccacggcg gcatccggcg ctcgtcgacc
    30301 gcctcggtga tcgcttcgcg gatcgggacg aagtcttgat acgtcgtcag cgcgaacggt
    30361 gcgatgccac tgtcggtatg gcagccggcg cacgtcgtcg tgacgagcgg ctcgatgtcg
    30421 cgataccagg tcggcgtcgc gacgcccgag gtgccgtcgt cggccagcgc g
//
LOCUS       DASUFX010000250         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_37897,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000250 DASUFX010000000
VERSION     DASUFX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37897"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1091
                     /locus_tag="VFV99_10070"
     CDS             156..1091
                     /locus_tag="VFV99_10070"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HEX5059696.1"
                     /translation="MAMLEFSKMLGDARRVLVAGCGGGYDVLGAVPLRHALRAAGIEV
                     HLASLSFAYLNGLTNAVQDAAVPNLYAVGANAATTRAYCPEAHLAKFFDAEDGGAHVV
                     WSFDKTGVRPLAAAYRALVDRLHIDAIVLVDGGIDAVLRGDETSLGTPSEDLATLAAA
                     TSLGIPVLLACIGMTAELRDGIAHAQVFERIAELSREGAYLGASALVPGTPACDVYVR
                     AVEAVFAGQSEQKQSHVHRVITRAVRGEFGATAPYVWLSPLASVFWFFDARVVARTHC
                     FLDELRATDSIWDVAARIEAIRKTLSIKERSTIPI"
     gene            1124..1993
                     /locus_tag="VFV99_10075"
     CDS             1124..1993
                     /locus_tag="VFV99_10075"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX5059697.1"
                     /translation="MRCPCLFLLAVGCGHGDTLVTSSAPTPTEAAVPKAEPASPCTSG
                     VTKLGPGLTAEQLTLIATPAAGAPCIDVVRADLAHHRLRVLTAAREGRSQPAPTWREM
                     FHLVAVINAGMFHNDGDPVALIIEDGVAVSADNKKYQGYLAFDPIAATDTPAIMTGRD
                     CPGFSLADLKKRYRSIVQGPRLLGCTGEALPWADKKQYSSAAIGLDRMGRGVFLHARA
                     AVTMTELSQALASVDLVGALFLEGGPEASLVVRGPDGELSRVGSYETSFVENDTNQSF
                     WWLPNVIGLETRD"
     gene            2038..>2608
                     /locus_tag="VFV99_10080"
     CDS             2038..>2608
                     /locus_tag="VFV99_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059698.1"
                     /translation="MGPKDPGDHATIDQSWDVSDARSPVDLDTDLQEKVDTPAIDPLE
                     AALQTFETQTSDKNPAQVQANDRTVAGLGVTTPAPSPVLKTSALDYPPDLSPRAFNSR
                     NPRPRDTKPPVLREPGKGPVTLERANELAQPERSPFDLVMEGLGTTPRPKVEDNHDLS
                     RFVSTFSGDTPPPVAIENPLGSAMEPVQRK"
BASE COUNT          418 a          881 c          863 g          446 t
ORIGIN      
        1 cgcgtgcggc cgcgctcggt ggtgcagatg cggtcgcggt gatccgcgcg gcatggcaaa
       61 gcgctccgga cctgatcggt aagctcgtgg aggcagtcga cgccggtgtg cgtgcgcgat
      121 agtgcttgcc gacttcgtgt actcatgaca cttttatggc gatgctcgag ttctcgaaga
      181 tgctcggcga tgcgcggcgc gtgctcgtcg ccggctgcgg tggtggttac gacgtactcg
      241 gcgcggtgcc actgcgtcac gcgctgcgag cggcggggat cgaggtccat ctcgcgtcgc
      301 tgtcgttcgc gtacctcaac gggctcacga acgcggtgca agacgccgcg gtccccaacc
      361 tgtacgcggt cggcgcgaac gcggcgacca cgcgcgcgta ctgccccgaa gcgcacctcg
      421 cgaagttctt cgatgcagag gacggtggtg cccacgtggt gtggagcttc gacaagaccg
      481 gcgtgcgtcc gctcgccgcg gcctaccgcg cgctggtcga ccgtctacat atagatgcga
      541 tcgtgctggt cgacggcggc atcgatgcgg tactgcgcgg cgacgagacc tcgctcggca
      601 cgccgtcgga ggacctcgcc acgctcgcgg ccgcaacgtc gctcggcatc ccggtgctgc
      661 tcgcgtgcat cggcatgacc gccgagctgc gcgacggcat cgcgcacgcc caggtgttcg
      721 agcggatcgc cgagctgtcg cgcgaaggcg cgtacctcgg tgcgagcgcg ctcgtcccgg
      781 gcacgccggc gtgcgatgtc tacgttcgcg cggtcgaggc cgtcttcgcc gggcagtcgg
      841 agcagaaaca gagtcatgtc catcgcgtga tcacgcgtgc ggtgcgaggc gagttcggcg
      901 cgactgctcc gtatgtgtgg ctgtcgccgc tggcgagcgt gttctggttc ttcgatgcgc
      961 gtgtggtggc gcgcacccat tgcttcctcg acgagctgcg cgcgaccgac tcgatctggg
     1021 acgtcgccgc ccgcatcgaa gcgatccgca agacgctctc gatcaaagag cgatcgacga
     1081 tcccgatttg aaatgatctg cggtcctcgc gcgtttcagc ggcgtgcgtt gcccctgtct
     1141 ctttcttctc gccgtcggct gcggtcacgg cgacacgctc gtcacgtcga gtgcgccgac
     1201 gccgaccgaa gctgcggtgc caaaagccga gcccgcgtcg ccgtgcacat ccggcgtgac
     1261 gaagctcggc ccgggcctca ccgccgagca gctgacgctg atcgcaacgc ccgctgccgg
     1321 tgcgccatgc atcgacgtcg tgcgcgcgga cctcgcgcat catcgactcc gcgtgctcac
     1381 cgccgcgcgt gaaggtcgat cgcagccggc gccgacctgg cgcgaaatgt ttcacctcgt
     1441 cgcagtgatc aacgccggca tgttccacaa cgacggcgat cccgtcgcgc tgatcatcga
     1501 agacggcgtc gcggtctctg cggacaacaa gaagtaccag ggctatctcg cgttcgatcc
     1561 gattgcggcg accgacacac cggcgatcat gacggggcgc gactgtcccg gcttctcact
     1621 cgctgacttg aagaagcgct accgatcgat cgtgcagggc cctcgcctgc tcggctgcac
     1681 aggtgaggcg ctgccgtggg ccgacaagaa gcagtactcg tctgccgcga tcggtctcga
     1741 tcgcatgggt cgcggcgtct tcctccacgc tcgcgccgcg gtcacgatga ccgagctctc
     1801 ccaggcgctc gcctcggtcg atctcgtagg cgcgctgttc ctcgaaggcg gcccggaggc
     1861 ctcgctggtg gtgcgcggcc cggatggcga gctatcccgg gtcggcagct acgagaccag
     1921 ctttgtcgag aacgacacga accaatcgtt ctggtggctt ccgaacgtca tcggcctgga
     1981 aacccgggat tagtcggcga ttaccaccct acatcgctgc ctgttatgat catctgaatg
     2041 ggtccaaaag atccgggtga ccacgccacg atcgatcaat cgtgggacgt gagtgacgcg
     2101 cgcagtccgg tcgatctcga cacggacctg caggagaagg tcgacacgcc cgccatcgat
     2161 ccgctcgaag ccgcgctgca gacgttcgaa acgcagacca gcgacaagaa tcctgcgcaa
     2221 gtgcaggcca atgatcgaac ggtggcgggc ctcggcgtga caacgcctgc gccgagcccg
     2281 gtgctcaaga cgtccgctct cgattacccg cccgatctct cgccgcgcgc gttcaattcg
     2341 cgaaatccgc gcccgcggga taccaagccg cccgtcctcc gcgaaccggg caaaggtccg
     2401 gtcacgctcg agcgcgcgaa cgagctggct cagcccgagc gctcgccgtt cgatctcgtg
     2461 atggaaggcc tcggcaccac gccacggccg aaggttgaag acaaccacga cctcagccgg
     2521 ttcgtgagta cgttcagcgg cgacacgccg ccgccggtcg cgatcgagaa tccgctgggc
     2581 tcggcgatgg agccggtgca acgcaagg
//
LOCUS       DASUFX010000251         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1567915, whole genome shotgun sequence.
ACCESSION   DASUFX010000251 DASUFX010000000
VERSION     DASUFX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567915"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFV99_10085"
     CDS             <1..979
                     /locus_tag="VFV99_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX5059699.1"
                     /translation="GGDEPPPDAATAEADAYVMPGEDFCNATDPRAMAVDVVATPEAG
                     EQPYLAALASAQSSIDVQVYLMGYGGILDQLQAKARAGVAVRVILDRAKADTNQKYFD
                     MLTAAGAQVKWSDARFVYQHSKFLLIDNQVAVISTGNFSKTYSIDLERNFVATDRDPA
                     DLWDLHTMFEADWSGAGATYTMPCTRLVVSPINARARIVDLINSAQSTLELESMQFGD
                     SEVRAAVKSRVQSGVVVRALLADADWISANTYAAQYLKDLAVPVKWIPHLHTKVIIVD
                     GARAYLGSENLSSNSLDNNREVGLIVTDQSSIAPLTTTFESDFTAGTPF"
     gene            1000..1716
                     /locus_tag="VFV99_10090"
     CDS             1000..1716
                     /locus_tag="VFV99_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059700.1"
                     /translation="MSSRTWQMIAAFISIGVMAFVLFTYGLRDDSPPPTSKKTEQPHA
                     GAQHFTAEKPEHEGGKLSPLQVYENAKVGEWRAYNVITESSLAQTFHAIGLETITKVD
                     DKTVSREFSGRVEETGQEQKRPYEDRPRQGLTIDELTTNDVGGWTIYELSITDEDHAI
                     GGRTFKCKKLSYASNDPMFPSKRTHTDVWISAEVLGGGIVEEREVQEMPEARFVITQQ
                     LIGFGPDDKTTTWGERPAGL"
     gene            complement(1723..1971)
                     /locus_tag="VFV99_10095"
     CDS             complement(1723..1971)
                     /locus_tag="VFV99_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059701.1"
                     /translation="MSVWKELGRFLERGTPLLEEAVKSLQDTRALLALLRPTVEALPD
                     LVSDTRGLVGDARALLRELSPLLVDLTRRTLAELRDGQ"
BASE COUNT          399 a          665 c          674 g          324 t
ORIGIN      
        1 cggaggggac gagccgcccc ctgatgccgc gacggcggag gccgacgcgt acgtgatgcc
       61 cggcgaggac ttctgcaacg cgaccgatcc gcgcgcgatg gccgtcgacg tcgtcgcgac
      121 gccggaggcg ggcgaacagc catatctcgc cgcgctcgca tcggcgcagt cgtcgatcga
      181 cgtgcaggtc tacttgatgg gctacggcgg catcctcgat caactgcaag cgaaggcgcg
      241 cgcaggcgtg gcggtgcgcg tgatcctcga tcgcgcgaag gccgacacga accagaagta
      301 cttcgacatg ctcaccgccg ccggtgcgca agtgaaatgg agcgacgccc gcttcgtcta
      361 ccagcactcg aagttcttgc tgatcgacaa ccaggtcgcg gtgatctcga ccggcaattt
      421 cagcaagacg tattcgatcg acctcgagcg caacttcgtc gcaaccgatc gcgaccccgc
      481 ggatctctgg gacctgcaca cgatgttcga ggccgactgg tccggcgcgg gtgccacgta
      541 cacgatgccg tgcacgcgcc tcgtcgtgtc gccgatcaac gcgcgcgctc gcatcgtcga
      601 tctgatcaac agcgcgcagt cgacactcga gctcgagtcg atgcagttcg gtgactccga
      661 ggttcgcgcc gccgtgaaat cccgcgtgca aagcggggtc gtggtgcgcg cgctgctcgc
      721 ggacgccgac tggatctcgg cgaatacgta cgccgcgcaa tacctgaaag acctcgcagt
      781 gcccgtgaaa tggatcccgc acctgcacac gaaggtcatc atcgtcgacg gcgctcgcgc
      841 gtatctcggc tcggagaacc tgtcgtcgaa ctcgctcgac aacaaccgcg aggtcggcct
      901 catcgtcacc gaccaaagct cgatcgcacc gctcacgacg acgttcgaga gcgacttcac
      961 cgcgggtacg ccattctagt ttcgtggtaa ccctgcctcg tgagctcgcg cacgtggcag
     1021 atgatcgcgg cgttcatctc gatcggcgtg atggcattcg tgctgttcac gtacggcctg
     1081 cgcgatgact cgccaccacc gacgagcaag aagaccgagc agccgcatgc cggtgcgcag
     1141 cacttcaccg ccgagaagcc agagcatgaa ggcggcaagc tatcgccgct ccaggtctac
     1201 gagaacgcga aggtcggcga gtggcgcgcg tacaacgtga tcacggagtc gtcgctcgcg
     1261 cagacgttcc atgcgatcgg actcgagacg atcaccaagg tcgatgacaa gacggtgtcg
     1321 cgtgagttca gcgggcgcgt cgaagaaacc ggccaggaac agaagcggcc gtacgaggat
     1381 cggccgcgcc agggtctgac gatcgatgag ctcacgacca acgatgttgg cggctggacg
     1441 atctacgagc tctcgatcac cgacgaggat cacgcgatcg gcggtcgcac gttcaagtgc
     1501 aagaagctga gctacgcgtc caacgatccg atgttcccga gcaagcgcac gcacaccgac
     1561 gtgtggatct cggcagaggt tctgggtggc ggcatcgtcg aggagcgcga ggtgcaagaa
     1621 atgccggaag cgcgcttcgt gatcacgcag cagctgatcg ggtttggtcc cgacgacaag
     1681 acgacgacct ggggcgagag gccggccgga ctctagggct cgtcactgac cgtcgcgcag
     1741 ctcggcgagc gtgcgccgcg tcagatcgac gagcaaaggg ctgagctcgc ggagcagcgc
     1801 gcgcgcatcg ccgacgaggc cgcgggtatc actcaccaga tcgggcagtg cctcgaccgt
     1861 cggtcgcaat agcgcgagca gcgcgcgcgt gtcctgcagc gacttcaccg cttcttcgag
     1921 gagcggcgta ccgcgctcga gaaagcgacc gagctctttc cagacagaca cggacgtgcg
     1981 gcgctagagc ttcagtgcct ggacgcgcgc ggccgtcatc tcgtgcagcg tgcgcatcac
     2041 gctgtacgcg gtgagctcgg cc
//
LOCUS       DASUFX010000252         6591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_177484,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000252 DASUFX010000000
VERSION     DASUFX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177484"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFV99_10100"
     CDS             complement(<1..895)
                     /locus_tag="VFV99_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059702.1"
                     /translation="MRLPLACAVLIASTSARANPLEVFGMTSKRAGQANVGVAAADDA
                     SALYYDPAGLVATPGGELIVGTLGAYSHLSINDERAKLPERVGFQLGFRAGLPLGGAL
                     KDRFSVGIALHLLPHNVARIIAPAPDQLFYPYYGDRLARIAVLPGAAARLGGGISIGA
                     AINVLASLDGSITAAEGSTRAIDARVDERVPTVARVIAGAQWQATPVLRFGAVYRQRF
                     SAPFTTTARTMIAGEPIDLDLRAEGAFTPHQVFAGVHWTTEAFATAFDVGWSKWSGYP
                     GPYVEVTSQLPLVGPVPAQAPH"
     gene            969..2495
                     /locus_tag="VFV99_10105"
     CDS             969..2495
                     /locus_tag="VFV99_10105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059703.1"
                     /translation="MEAATRELAPGTMLDDKYRIESLLAVGGMGAVYVGTHTQLRKKV
                     AIKILNPQLNSPAMIARFQREAITASQIGHEGIAQVTDLGTSRDGEAFLVMELLEGES
                     LTRRLRAAGPMAIEQACELGCAILAPLQAAHQAGVVHRDLKPDNVFLVRQSRGELVKL
                     LDFGISRAAGLDAEMRLTNTGVVMGTPFYMSPEQARGDHEVGPASDIYSFGVILYEML
                     VGGLPITGENYNQLLYKVMTGDYVRARQRRPDIPEALDDIIAMSMALEADMRPPSAAE
                     LEHALLQFCRPQFRDHMIQRISSAGIVFGTSPPHRRSQPAGVRLDQGTDPTLVAPSAI
                     IPPKKRSKLPFILAGMLVVGGAGAAVVAMQSGKETKPLDTKTEPAVAATPTPTPPPAA
                     APTPAPVPAPPAAPTTITLKFAVDPATAVITVDGNAVKNGELVVEKDGAEHALSVTAP
                     GFKPHDEKLAFDESQKLVIHLDKVTKAATPTRPRPTKQDKPDKQETKHDRIESESPYK
                     "
     gene            2492..3553
                     /locus_tag="VFV99_10110"
     CDS             2492..3553
                     /locus_tag="VFV99_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059704.1"
                     /translation="MKRLPIDLRNAFALLRAPAKPGLAVMVLFLATPVLAAPKQNEQT
                     AEQKEADRHFKSGVALYKESKYAEALAEFERAYELVPHPLVLYNIATCHRELSHYGEA
                     VKYYQRFLKEGADKVPAARLKEAQSELDAVLQRIARVTVTIKGGEGAKIILDGEDVGT
                     MPIDGPLIVPPGEHRLVVRAEGKQDAERAIRVASGDEVQVTLQLTDAPKTGLVESLPP
                     PPPPPPKPKRFALGAAVGTNLLNVPETGAPTLGVGFKVHPHMMVGVDATLVAYAAMPN
                     VRVRLAGDQLSVHATVAIPIAFNDGNMSETFVAGAAGVGVRYRATPALAFRLEGFASY
                     GFSDHGFTLPAFFGGELWF"
     gene            3550..4815
                     /locus_tag="VFV99_10115"
     CDS             3550..4815
                     /locus_tag="VFV99_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5059705.1"
                     /translation="MRHALISLVLLAGCKQSLFDSHGEPDASTGDGGNFPSSCPATCL
                     GDAAADFGMPSWRYLDDTRNRAWAPMTMMGNDFVGMDNNRIWSCRKDGSPEACKMLPG
                     ALLVTNSGTSTAADPALSFTATANQVIQLSVRVYVPMGAQQQEVRLYRNAREDSLFTG
                     LAPAGGTFDRAITVDALNGDRFYLSVGGSTAGATEKIGVHFYVNATTDAFPKDCQLAL
                     PFAGPMANTTDNVCGVDFAYKNADTAATWALGQPPFPEMGTSADITRDKYFVGSDVLV
                     RSGDTTTQLWLRHDAFVDTYGTVVFSDDDLDNTGGLTIYVANTSPPKLGAETCTVVTQ
                     TTLDFAWAEGTYPDDHAWHFVRVVHTNGMVKICVDGARTASYALPAGKMQGMYPPWLG
                     RNLNWTPTGGFLDGAIDDVRVFTGALPCE"
     gene            4828..5736
                     /locus_tag="VFV99_10120"
     CDS             4828..5736
                     /locus_tag="VFV99_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059706.1"
                     /translation="MSTFACGHCGASLAFDRVRTETCPFCASPNFVERPPAAGQPDPQ
                     FVVAFVGDAEVARRALDRWLGSRSMFADSALRRGRVEAMRGIYVPAYLYSAVAHTDYT
                     AQIGEHYTESEEYTHTTPDGKTETRTRTVTRTEYRPLSGYHVGYVTDVVVSASQGLSN
                     DELGRIEPFDFKQMRRFEHALVSGWIVEELAWTADECQHACRTEAVDHVGSKLRRFMP
                     GDSYSDLSWRTNVAWESLDPILVPVWVLALRYRDNKDPLRVVINGQTGRVGGRVPLSW
                     WKVTIGVIVLAAIAAAIAYLIYTRQR"
     gene            5733..>6591
                     /locus_tag="VFV99_10125"
     CDS             5733..>6591
                     /locus_tag="VFV99_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059707.1"
                     /translation="MTVAVAAAVGCSRCASPLEDGDLRCAVCALPVPVAPARIDKVRV
                     QVLRCTWCGAAIAYDANHQAPRCGFCSSVMTIEQPVDPIEAATLRVPFLVDREAATAA
                     LHTWLGKRGYFAPATLRDEAVLETITPLCWAAWVVNAEAVVAWTADSDYGSGQSRWAP
                     HSGHVKQSFGNIVVPASRGLRHDECTSLVPYYDLARLVPVDAAEIPGEVPAMVERFDA
                     QRSAARQHVSRAIEALAKVRVQKSEVPGRRFRNVHVACLLERQTTDRVALPAWVLAYR
                     YRGSPYRAIV"
BASE COUNT         1121 a         2239 c         2208 g         1023 t
ORIGIN      
        1 cgtgcggcgc ttgtgccggc accggaccga cgagcggcaa ctggctcgtc acctcgacgt
       61 agggcccggg atagcccgac cacttcgacc agccgacgtc gaaggcggtc gcgaacgcct
      121 cggtcgtcca gtgcacgccg gcgaacacct ggtgcggcgt gaacgcgccc tcggcgcgca
      181 gatcgagatc gatcggctcg cccgcgatca tcgtccgcgc ggtcgtcgtg aacggcgccg
      241 agaagcgctg gcggtaaacc gcgccgaacc gcaggaccgg cgtggcctgc cattgtgcgc
      301 ccgcgatcac acgcgccacg gtcggaacgc gctcgtcgac ccgcgcatcg atcgcacgcg
      361 tcgaaccctc ggccgccgtg atcgagccat cgaggctcgc gagcacgttg atcgcggcgc
      421 cgatcgagat gccgccgccg agccgcgcgg ctgcgcccgg caacaccgcg atgcgtgcga
      481 gccgatcgcc gtaatacgga tagaacagct ggtcgggcgc cggcgcgatg atgcgcgcga
      541 cgttgtgagg caacaggtgg agcgcgatac cgacgctgaa gcggtctttc agcgcgccgc
      601 cgagcggtaa gccggcgcgg aacccgagct ggaagccgac gcgctcggga agcttcgcgc
      661 gctcgtcgtt gatcgagagg tgcgaatacg cgccgagtgt gccgacgatg agctcgccgc
      721 caggcgtggc gacgagcccg gctgggtcgt agtagagcgc ggacgcatca tctgcagccg
      781 cgacgccgac gttcgcctgg cctgcgcgct tcgacgtcat cccgaacacc tcgagcgggt
      841 tggcacgcgc agatgtgctc gcgatcagca cagcgcacgc caatggcaaa cgcacgaggc
      901 ccaccgttat tggaaggtgc cacatgtcgg cgtgaataaa tggccggcgt ggcttattct
      961 tatgggcggt ggaggccgct acgcgcgaat tggcgccggg cacgatgctc gacgacaagt
     1021 accgcatcga aagcttgctc gccgtgggtg gcatgggcgc cgtctatgtc ggcacccata
     1081 cccagctgcg caagaaggtc gcgatcaaga tcctcaatcc gcagctgaac agccccgcga
     1141 tgatcgcgcg gtttcagcgc gaggcgatca ccgcgtcgca gatcggtcac gagggcatcg
     1201 cgcaggtcac cgacctcggt accagtcgcg acggtgaagc gtttctcgtc atggagctgc
     1261 tcgaaggcga gtcgctcacc cggcggttgc gcgctgcggg acccatggcg atcgagcagg
     1321 cgtgcgagct cggctgcgcg atcctcgcgc cgctgcaagc cgcgcatcag gccggcgtcg
     1381 ttcaccgcga tctcaagccc gacaacgtgt tcctcgtgcg ccagagccgc ggcgagctgg
     1441 tcaagctgct cgacttcggc atctcgcgcg cggccggtct cgatgccgag atgcggctga
     1501 cgaacaccgg cgtcgtgatg ggcacgccgt tctatatgtc gcccgagcaa gcccgcggcg
     1561 atcacgaggt tggtcccgcg tcggacatct actcgtttgg cgtcatcctc tacgaaatgc
     1621 tcgtcggcgg gctgccgatc acgggcgaga actacaacca gctcctctac aaggtgatga
     1681 ccggcgacta cgtgcgcgcg cgccagcgcc ggccagacat tcccgaggca ctcgacgaca
     1741 tcatcgcgat gtcgatggcc ctcgaggccg acatgcggcc gccgagcgca gccgagctcg
     1801 aacacgcgct gctccagttc tgccggcccc agtttcgcga tcacatgatc cagcgcatct
     1861 cgtcggcggg catcgtgttc ggcacgtcgc cgccgcatcg ccggtcgcag cccgcgggcg
     1921 tgcgactcga tcagggcacc gatccgacgc tcgtcgcacc gagcgcgatc attccgccca
     1981 agaagcgctc gaagcttccg ttcatcctcg cgggcatgct cgtcgtcggc ggtgcgggcg
     2041 ctgccgtcgt ggcgatgcag tccggcaagg agacgaagcc actcgacacg aagaccgagc
     2101 cggcggtcgc ggcgacaccg acaccaacac caccgccggc ggctgcgccg acgccagctc
     2161 ccgttcctgc gccgccagca gcaccgacga cgatcacgct caagttcgcg gtcgatcccg
     2221 caaccgcagt gatcacggtc gatggcaacg cagtgaagaa cggcgagctc gtcgtcgaga
     2281 aggatggcgc cgagcacgcg ctcagcgtca ccgcgccggg cttcaaaccg cacgacgaga
     2341 agctcgcgtt cgacgagagc cagaagctcg tcatccatct cgacaaggtc acgaaggcgg
     2401 caacgccgac gcggccgcgt ccgacgaagc aagacaagcc agacaagcag gaaaccaaac
     2461 acgaccgcat cgagagcgag agcccctaca aatgaagcgc ttgccgatcg acctgcgcaa
     2521 cgcattcgcg ttgctgcgcg ccccggcaaa gccgggactt gcggtgatgg tgttgttcct
     2581 cgcgacgccg gtcctggcgg cgccgaagca aaacgagcag acggcggaac agaaggaggc
     2641 ggaccgtcac ttcaagagtg gcgtcgcgct ctacaaggaa tcgaagtacg ccgaggcgct
     2701 cgccgagttc gagcgtgcgt acgagctcgt gccgcacccg ctcgtcctct acaacatcgc
     2761 gacgtgccat cgcgagctgt cgcactacgg cgaagcggtg aagtactacc agcgcttcct
     2821 caaggaaggc gctgacaagg ttccggcggc gcgcctcaag gaagcgcaga gcgagctcga
     2881 cgccgtgctt cagcgcatcg cccgcgtgac ggtcacgatc aaaggcggcg agggtgcgaa
     2941 gatcatcctc gacggcgaag acgtcggcac gatgccgatc gacgggccgc tgatcgtgcc
     3001 gcccggcgag caccggctgg tcgtgcgcgc cgaaggcaag caggacgccg agcgcgcgat
     3061 tcgcgtcgca tccggtgacg aggtccaggt cacgctgcag ctcaccgatg cgccgaagac
     3121 gggtctcgtc gagtcgttgc cgccgccgcc accaccgccg ccgaaaccga aacgttttgc
     3181 actcggcgca gcggtcggca cgaacctgct caacgtgccc gagaccggcg caccgacgct
     3241 cggcgtcggc ttcaaggttc acccgcacat gatggtcggc gtcgatgcaa cgctcgtcgc
     3301 gtacgcggcg atgccgaacg ttcgcgtgcg gctcgccggc gaccagctgt cagtccacgc
     3361 gacggtcgcg attccgatcg cgttcaacga cggcaacatg agcgagacgt tcgtcgccgg
     3421 tgccgccggc gtcggcgtgc ggtatcgcgc gacgcccgcg ctcgcgttcc gtctcgaggg
     3481 cttcgcgagc tacggcttct ccgaccatgg cttcacgctg cccgcattct tcggaggtga
     3541 gctgtggttt tgagacatgc gctcatcagc ctggtcctgc tcgcaggctg caagcagtcg
     3601 ctgttcgaca gccatggcga gccagacgcg agtaccggcg atggcggtaa ctttccgtcg
     3661 agctgcccgg cgacctgtct cggtgatgcc gcggcggact tcgggatgcc gagctggcgt
     3721 tacctcgacg acacgcgcaa ccgcgcgtgg gcgccgatga cgatgatggg caacgacttc
     3781 gtgggcatgg acaacaaccg catctggtcg tgtcgcaagg acggttcgcc cgaagcgtgc
     3841 aagatgctgc cgggcgcact gctcgtgacg aactcgggca cgtcgaccgc cgccgatccg
     3901 gcgctgtcgt tcaccgcgac cgccaatcag gtcatccagc tctcggtgcg cgtctacgtg
     3961 ccgatgggcg cgcagcagca ggaagtgcgg ctgtaccgca acgcgcgcga agactcgctg
     4021 ttcaccgggc tggcgccagc gggcggcacg ttcgatcgcg cgatcaccgt cgacgctctc
     4081 aacggcgacc gcttctacct ctcggtcggc ggctcgacgg ccggtgcgac cgagaagatc
     4141 ggcgtgcact tctacgtcaa cgcgacgacg gatgcattcc cgaaggactg ccagctcgcg
     4201 ctgccgttcg ccggaccgat ggcgaacacg accgataacg tgtgcggcgt agatttcgcg
     4261 tacaagaatg cggacacggc ggcgacgtgg gcactcggtc agccgccatt ccccgagatg
     4321 ggaacgtccg ccgacatcac acgggacaag tacttcgtcg gcagcgacgt gctcgtgcgg
     4381 agcggtgaca cgacgacgca gctgtggctg cgccacgacg cgttcgtcga tacgtacggc
     4441 acggtcgtgt tctccgacga cgacctcgat aacaccggcg gcctcacgat ctacgtggcc
     4501 aacacgtcgc cgccgaagct aggcgccgaa acctgtacgg tcgtcaccca gacgacgctc
     4561 gacttcgcat gggcggaggg cacgtatccg gacgaccatg cgtggcactt cgtccgtgtc
     4621 gttcacacga acggcatggt gaagatctgt gtcgacggtg cgcgcaccgc gagttacgcg
     4681 ctcccggccg gcaagatgca gggcatgtat ccgccgtggc tcggtcgcaa cctgaactgg
     4741 acgccgactg gcggattcct cgacggcgcg atcgacgacg tgcgcgtgtt caccggcgcg
     4801 ctgccctgcg agtagtacac tggtggcgtg agcacgttcg cgtgtgggca ttgcggtgcg
     4861 agcctcgcgt tcgatcgcgt gcgcaccgag acgtgcccgt tctgcgcgag cccgaacttc
     4921 gtcgagcggc cgccggccgc gggccagccc gatccgcaat tcgtcgtcgc gtttgtcggc
     4981 gatgccgagg tcgcccgccg cgcgctcgat cgctggctcg gcagccggtc tatgttcgcc
     5041 gactccgcac tgcgccgggg acgggtcgag gcgatgcgcg ggatctacgt gccggcgtat
     5101 ctctactccg cggtcgcgca caccgactac accgcgcaga tcggcgagca ctacaccgag
     5161 agcgaggagt acacgcacac gacgcccgac ggcaaaaccg agacgcgtac gcggacggtg
     5221 acccgcaccg agtatcgacc gctctccggc tatcacgtcg gctacgtcac cgacgtcgtc
     5281 gtcagcgcat cgcaaggttt gtcgaacgac gagctcggcc gcatcgagcc gttcgatttc
     5341 aagcaaatgc ggcgcttcga gcacgcgctc gtgtcgggct ggatcgtcga ggagctcgcg
     5401 tggaccgccg acgagtgcca gcacgcctgc cgcaccgaag ccgtcgatca tgtcggcagc
     5461 aagctgcgcc gcttcatgcc gggcgatagc tacagtgatc tgtcgtggcg cacgaacgtc
     5521 gcgtgggaat cactcgatcc gatcctcgtg ccggtctggg tgctcgcgct gcgctatcgc
     5581 gacaacaagg acccgctgcg tgtcgtgatc aacgggcaga ccggccgagt cggcggcaga
     5641 gtgccgctgt cgtggtggaa ggtgacgatc ggcgtcatcg tgctcgccgc gatcgccgca
     5701 gcgattgcct acctcatcta cacgaggcaa cgatgacggt cgcggtcgct gctgccgtcg
     5761 gctgtagccg ctgcgcgagc ccgctcgaag acggcgacct gcgctgcgcc gtctgcgcgt
     5821 tgccggtgcc ggtcgcgccc gcgcgcatcg acaaggtgcg cgtgcaggtg ttgcgctgca
     5881 cgtggtgcgg cgctgcgatc gcgtacgacg cgaaccatca ggcacccagg tgcggtttct
     5941 gcagctcggt gatgacgatc gagcagccgg tcgatccgat cgaagcagcg acgctgcgcg
     6001 tgccattcct cgtcgatcgt gaagcggcaa ccgccgcgct tcacacctgg ctcggcaagc
     6061 gcggttactt cgcgccggcg acgctgcgcg acgaggcggt gctcgagacg atcacgccgc
     6121 tgtgctgggc cgcgtgggtc gtcaacgccg aggcggtcgt cgcgtggacg gccgattctg
     6181 actacggctc cggtcagtcg cgctgggctc cgcactccgg ccacgtcaaa caatcgttcg
     6241 gcaacatcgt cgtgcccgcg tcacgtggtc tgcgccacga cgagtgcacg tcgctcgtgc
     6301 cgtactacga cctcgctcgc ctcgttcccg tcgacgcggc ggagattccc ggcgaggtgc
     6361 cggcgatggt cgagaggttc gacgcgcagc gctcggccgc ccgccagcac gtgagccgtg
     6421 cgatcgaagc gctcgcgaag gtgcgcgtgc agaagtcgga agtgccgggc cgccgcttcc
     6481 gcaacgtgca tgtcgcgtgc ttgctcgagc ggcagaccac cgatcgcgtc gcactgccgg
     6541 cgtgggtgct cgcgtatcgc taccgcggct cgccgtatcg cgcgatcgtg c
//
LOCUS       DASUFX010000253         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_873034,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000253 DASUFX010000000
VERSION     DASUFX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873034"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFV99_10130"
     CDS             <1..332
                     /locus_tag="VFV99_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059708.1"
                     /translation="NVDAAITLSELLKALGHEPLVAHDAPTALELAREEAPQLALVDL
                     GLPLLDGYELIARLRNLPGLHSIPAVAVTGYGQQSDRDRSQAAGFDRHVVKPVGLDDL
                     RTLLASL"
     gene            344..1150
                     /locus_tag="VFV99_10135"
     CDS             344..1150
                     /locus_tag="VFV99_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5059709.1"
                     /translation="MPKLEVNGTTLYYEDTGPGSTGETIVFSHGLLWGTELFEPQINA
                     LRGRYRCIAWDHRGQGRSAEDPHRHCIGIELVWQDAVALLDALQIKGAHFVGLSMGGF
                     VGMRVAARRPDLVKKLALLETSYEPEPTANVKRYRLLNTIYKTIGPRVIASKVAPIML
                     GKSIINDPSRKGEVARFVKLMTRRRDIWRAVNGVIDRAGISQHELAQISAPTLVVVGD
                     EDVATPHSKAEQIATAIRGARLVTIPRAGHSSPVEEPVAVTTALESFIGG"
     gene            1302..1700
                     /locus_tag="VFV99_10140"
     CDS             1302..1700
                     /locus_tag="VFV99_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX5059710.1"
                     /translation="MSEVRVVIQREADIVLARQAGRQLAAQLGFSVSDQTLIATAISE
                     VARNIVVYAVHGEIVLMRFDDGDRIGIQVQAVDNGPGIDNKELAMRDGYSTKNSLGLG
                     LPGARRLMDDFALDSEVGRGTTVTMKKWKR"
     gene            1688..2674
                     /locus_tag="VFV99_10145"
     CDS             1688..2674
                     /locus_tag="VFV99_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059711.1"
                     /translation="MEALMAMTRPDPKRMQLEHEYSSALRDCVGGAGEVALSHAYELG
                     RMAAGTGIGVVELAAIHDTALREVLGQSAPPPEVDQFFAEALSPFEMTHRGFREANER
                     LEAAVRELESFSYSVSHDLRAPLRTISAFTQALADDLRYQLDDKAKDHLRRVLAAAAR
                     MSDLIDALLELSRISRQPLGRHAVDISAVALAAVDDLRRRDVTRKLGATVQAGLVAEA
                     DGRLVRILFDNLIGNAWKFTAKTENPHVIVGAERRDAETVFFVRDNGAGFDMTYVDRL
                     FTPFQRLHSEREYGGTGIGLATVKRIVERHGGRIWAESAVGQGASFYFTLPA"
     gene            2709..2957
                     /locus_tag="VFV99_10150"
     CDS             2709..2957
                     /locus_tag="VFV99_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5059712.1"
                     /translation="MSAEEHKEPLELMPIERELLARMSHELRTPLNAIIGFAELLYDG
                     HVDPASLQHKEFIGDILANGRHLLKLIDDALGRVNTDE"
     gene            2960..3331
                     /locus_tag="VFV99_10155"
     CDS             2960..3331
                     /locus_tag="VFV99_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059713.1"
                     /translation="MRERVLIVDDNPTNLKLVSFLVKANGYEVDTAIDADAAIAAIAA
                     QRPRVILMDLQLPGIDGLELTRRLKADPATRDISIIAVTAYAMKGDQERARAAGCDDY
                     VTKPIDTRALPELIARYVKAT"
     gene            complement(3361..4404)
                     /locus_tag="VFV99_10160"
     CDS             complement(3361..4404)
                     /locus_tag="VFV99_10160"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5059714.1"
                     /translation="MIRGLTILFFVATTTPAFAHKPSDAHLRLDVDGDQLVGRLDVSV
                     RDLDAAISIDDGDGGLTWGELEAAAPRTREYIASRLGFGPGCALTQGDPSLLDLSDGA
                     YWSTPLTVHCAPRTLSIAYSLFFDIDAQHRGLVHLGSQTLIVRDAAPVTAQLGNTTSL
                     ATFVREGVWHIWIGIDHILFLVCLILPAVWPSGGRKTLRDVCVEVFEIVTAFTLAHSI
                     TLVISAVGLFTLPSRFVETAIALSVVAAAVNNLVRAIDARWAVAFALGLLHGFGFSSV
                     LIDLGLPSRELIGALLGFNIGVELGQAAIVVTLIPLLYWIRNTFAYRALLFAGSGAVA
                     VLASIWTYQRFVL"
BASE COUNT          875 a         1405 c         1509 g          704 t
ORIGIN      
        1 acaacgtcga cgccgcgatc acgctcagcg agcttctaaa ggcactcggt cacgagccgc
       61 tcgtggcgca cgatgcgccg acagccctcg agctcgcgcg cgaagaagcg ccccagctgg
      121 cactcgtcga tcttggctta ccgctgctcg atggctacga gctgatcgcg cggctgcgca
      181 atcttcccgg tctgcactcg atcccggcgg tcgcggtcac tggctacggc cagcaaagcg
      241 atcgcgatag gagtcaggcg gcgggcttcg atcgccacgt cgtcaagccg gtcggtctcg
      301 acgacctacg cacgctgctc gcatcgctgt aggattcggc gcgatgccga agctcgaggt
      361 gaatgggaca acgctgtact acgaggacac tggaccgggc tcgaccggcg agacgatcgt
      421 gttctcgcac gggctcttgt ggggaaccga gctgttcgaa ccgcagatca atgcgctgcg
      481 cggacgctat cgctgcatcg cgtgggatca ccgcgggcaa ggcaggagcg ccgaggatcc
      541 gcatcgccac tgcatcggca tcgagctggt gtggcaagac gcggtcgcgt tactcgacgc
      601 gctgcagatc aaaggcgcgc acttcgtcgg gctgtcgatg ggcggtttcg tcggcatgcg
      661 cgtcgcggcg cggcgaccgg atctggtgaa gaagctcgcg ttgctcgaga cctcgtacga
      721 gcctgaaccg accgcgaacg tgaagcgcta tcgcctgctc aacacgatct acaagacgat
      781 cgggccgcga gtcatcgcga gcaaggtcgc gccgatcatg ctcggcaagt cgatcatcaa
      841 tgatccatcg cgcaaaggcg aggtcgcgag gttcgtgaag ctgatgacgc gccggcgcga
      901 tatctggcgc gccgtcaacg gcgtgatcga tcgggcaggg atctcgcagc acgagctcgc
      961 ccagataagc gcgcccacgc tcgtcgttgt cggagatgaa gatgtggcaa caccgcacag
     1021 taaggcggag cagatcgcca cagcgatccg aggtgcgcga cttgtcacga ttcctcgtgc
     1081 aggacattcg tcgcccgtcg aagagcccgt tgccgtcact acagcattgg agtcattcat
     1141 tgggggctag gactcactaa cgctcgcctg tgtacgtagt ccacgggtac aactgcattc
     1201 gaggcggtcg cgggtttgca aagaagccga acgtgacgga tgcggatgcc aaccttccag
     1261 caacgagaaa cgccggccga gcgcgaggga gcggccgacc tgtgagcgaa gtccgcgtcg
     1321 tgatccaacg cgaggcagac atcgtgcttg cacggcaagc cggtcggcag ctcgcagcac
     1381 aactgggatt ctcggttagt gatcagacgc tgatcgcgac cgcgatctcc gaagtcgcgc
     1441 gcaacatcgt cgtgtacgcg gtgcacggcg agatcgtgct catgcgcttc gacgacggcg
     1501 atcgcatcgg tatccaggtg caagccgtcg ataacgggcc gggcatcgac aacaaagagc
     1561 tcgcgatgcg cgatggctat tcgacgaaga acagcctcgg gctcgggcta cccggcgcac
     1621 gccgcttgat ggacgacttc gcgctcgatt cggaggtcgg gcgcgggacg accgtcacga
     1681 tgaagaagtg gaagcgctaa tggcgatgac gcggccagac cccaagcgca tgcagctcga
     1741 gcacgagtac tcgagcgcgt tgcgcgattg tgtcggcggt gcaggagagg tcgcgctctc
     1801 gcatgcgtac gagctgggcc gcatggcagc cggcaccggc atcggtgtcg tcgagctcgc
     1861 cgcgatccac gacacggcgt tgcgcgaggt gctcggccag agcgccccgc cacccgaagt
     1921 cgatcagttt ttcgccgagg cgctgtcgcc gttcgagatg acgcatcgtg gtttccgcga
     1981 ggcgaacgaa cgactcgagg cggccgtgcg cgagctcgaa tcgttcagtt actcggtctc
     2041 gcacgatcta cgtgcgccgc tacgcacgat cagtgcattt acccaggcgc tcgccgatga
     2101 cctgcgatac cagctcgacg acaaggcgaa agatcatctg agacgcgtgc tcgcggctgc
     2161 ggcgcggatg agcgacctga tcgacgcact gctcgagttg tcacgaatca gccgccagcc
     2221 gctcggccgt cacgcggtgg atatctcggc cgtcgcactc gccgctgtcg atgacctgcg
     2281 gcgccgagac gtcacgcgga agcttggcgc gaccgtgcag gccggccttg tcgccgaggc
     2341 cgacggccgt ctcgtccgca ttctgttcga caacctgatc gggaacgcct ggaagttcac
     2401 cgcgaagacg gaaaaccctc acgtcattgt cggcgccgaa cggcgggacg ccgagaccgt
     2461 attcttcgtg cgcgacaacg gcgccggatt cgacatgacg tacgtcgatc gcttgttcac
     2521 gccgtttcaa cggctgcaca gcgagcgcga gtacggcggc acgggcatcg ggctcgcgac
     2581 cgtgaagcga atcgtcgaac gtcacggcgg aagaatctgg gccgagagcg cagtcggtca
     2641 gggtgcgagt ttctacttca cactccccgc gtagcgcggg tgctaacgtg caccgcaact
     2701 caagacctat gtcggccgaa gaacataaag agcctctcga gctgatgccg atcgagcgcg
     2761 agctgctcgc gcgcatgtcg cacgagctgc ggacgccgct caacgcgatc atcggctttg
     2821 cggagctgct ctacgacggt cacgtcgatc cggcgtcgct gcagcacaag gagttcatcg
     2881 gcgacatcct cgccaacggg cgccatctgc tgaagctgat cgacgacgca ctcggccgcg
     2941 tgaacacgga cgagtgacga tgcgtgagcg cgtattgatc gtcgacgaca acccgacgaa
     3001 cttgaagctc gtctcgtttc tcgtgaaggc gaacggttac gaggtcgaca ccgcgatcga
     3061 tgccgatgcc gccatcgcgg cgatcgcagc gcagcggccg cgcgtgatct tgatggacct
     3121 ccagctgccg ggcatcgacg ggctcgagct gacgcgccgg ttgaaagcag acccggcgac
     3181 gcgcgacatc tcgatcatcg cggtgactgc atacgcgatg aaaggcgatc aggagcgagc
     3241 gcgcgctgcc ggttgcgacg actacgtcac gaaaccaatc gacacccgcg cgttaccgga
     3301 gctcatcgcg cggtacgtca aggcgacgta gagctgcgcc tcgtacgcgc ttgcgttggt
     3361 tcagaggacg aagcgctggt acgtccagat cgacgcgagc acagcgaccg ccccggaacc
     3421 cgcgaacagc agcgcgcggt aggcgaacgt gttgcgaatc cagtagagga gcggaatcag
     3481 cgtgacgacg atcgcggcct ggccaagctc gacgccgatg ttgaagccga gcagtgcgcc
     3541 gatcagctcg cgcgacggga ggccgagatc gatcagcacg ctcgaaaagc cgaagccgtg
     3601 gagcagaccg agcgcgaacg cgaccgccca ccgcgcgtcg atggcacgca cgaggttgtt
     3661 caccgccgcg gcaacgaccg atagcgcgat cgcggtctcg acgaagcgcg acggcagcgt
     3721 gaacaaaccg accgccgaga tcacgagcgt gatcgagtgg gcgagcgtga acgcggtgac
     3781 gatctcgaat acttcgacgc agacgtcgcg cagcgtcttg cggccaccgc tcggccatac
     3841 cgccggcagg atcaagcaga cgaggaacag gatgtgatcg ataccgatcc agatgtgcca
     3901 gacgccttcg cgaacgaacg tggcgagcga ggtcgtattg ccgagctgcg cggtgaccgg
     3961 cgcggcgtcg cgcacgatca acgtctggct gccgaggtgg acgaggccgc gatgctgggc
     4021 atcgatgtcg aagaacagcg agtacgcgat cgacagcgtg cgcggtgcgc agtggacggt
     4081 gagcggcgtc gaccagtacg cgccgtcaga gagatcgagg agcgacgggt cgccctgcgt
     4141 cagcgcgcag ccggggccga acccgagccg gctcgcgatg tactcgcgcg tgcgcggtgc
     4201 ggccgcctcg agctcgcccc aggtcagccc gccgtcgcca tcgtcgatcg agatcgcggc
     4261 gtcgagatca cggaccgaga catcgagccg gccgacgagc tgatcaccgt cgacatcgag
     4321 ccggaggtgc gcgtcgctcg gcttgtgcgc gaacgcaggt gtggtcgtcg cgacgaagaa
     4381 cagaatggtc agcccacgaa tcacgagcaa cctccgcgac gaaacatgtg gagcagagtg
     4441 gtgagcgctt cgagcgcggg ttgggtcgga gccgttggcg atgcttggct ccg
//
LOCUS       DASUFX010000254         5667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1427680, whole genome shotgun sequence.
ACCESSION   DASUFX010000254 DASUFX010000000
VERSION     DASUFX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427680"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFV99_10165"
     CDS             <1..464
                     /locus_tag="VFV99_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059715.1"
                     /translation="DKVTKVIRVGPDPWRLYMSHDGKYGITANNGDSTISIIDIAKNA
                     VVATYPVGPDLTGINFAAGKAFVISSTTGFLYVFDMSTLKPAGRIKIGNNLFLQTATT
                     DTADQKLYLGSATDNSVYIIDGATEAVERVANVGQFPWGTHIMDSKDNYCH"
     gene            578..1360
                     /locus_tag="VFV99_10170"
     CDS             578..1360
                     /locus_tag="VFV99_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059716.1"
                     /translation="MKPQSSMVHSRSLLLAGRLAGLALLAVAAGADSSPSAFAQEPQA
                     QASDSPPDAAAPPAQGSGVRAATRLVRRSATAVTEEQASELTLTLTVAESRPIQTWVR
                     TAGVLDTAGKVLTAQLRPAEAELVEVGQRVRTFSVNSRSQMVQAKVTRVTPRPGGATV
                     EATLATAVANNGARHLMEIVTEKGPFLSVPNEAIIEEGESQVVYLKLPSGEFAPRTIH
                     TGITGELYTQIVDGLEDGDEVVSIGSFFVDADNKLKGGPTEP"
     gene            1357..4458
                     /locus_tag="VFV99_10175"
     CDS             1357..4458
                     /locus_tag="VFV99_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX5059717.1"
                     /translation="MNPALFEGIVHARWLIVLLILAGVIASVFAMRAAPLDAIPDTSD
                     PQVIVYAKWARSPELLEEQVTEPVVKALIGSPEVRSIRATSHMGYSFIYVILRSTSDR
                     AAVRQLIADRLATIRQLLPADATVSIGPNASGIGWIYQYALIDHEKLRDPREMRLLNE
                     TQLKPALQALPGVAEVASVGGLEKQYQLKLFPPLLADAGISLRELAAALRDALEEAGG
                     RTIEVTNRDYQVRGVVNHDNIDDLEALIVGHAPDGRAIRVKDVGYIQIGYDQRRGIAD
                     LNGDGEVVGGIVIMEQSQNALEITAALKKRLAEVAASLPPGIEIATTYDRSQLILGTL
                     RHFFLTLAYELGVVILVTALFLRNARTSLAPVAILLLGVLFTAIPMALLSQTINLFSL
                     AGLFIAIGEMVDATIVIVENSTAELAAHPGASENEKRQIVLRSIAGVAKPLLFSLLII
                     LASFLPVFFLDDKEARMFDPLAYSKTFAMAFSTLLTLCVLPIAMLWVFKGQVIHGPSR
                     FELGFTNWYRRATQWFVRYRYAFLAVNVVLLVAAVVLLQGFRRDFMPRMEEGSILYMP
                     TTLPGVPMKEAGWALQQMDKKLAAFPEVASVFGKLGRADTATDAAPVSMIEATIQLKD
                     REHWRPNLTKDQLVAEMDAAMQVVGYANSWVQPINARTVMQDTGIQTPTGIKVKGPDV
                     ATIESIGEEIEQLLRPYPGTQSVIAERISSGYFIDVEFDAQRLAAAGLSAQEAMPVVR
                     FGVGGDNLLAIKDSSGAQIPLSLQYSPEYLDTLEKVRTSPVVGPDGTTVALGEIADVA
                     VKKMPDMLRNDNGTLAGYVYVYLNGDVTATDYVAGAQAFLAENLALPSGYSIEWTGDY
                     QNALAAAKRLEIVIPTTLVVIFVLLVLAFRSIADSLLIMLSVPFALTGGVFLQWLLGY
                     PMTTAVIVGYIALFAVAIQTGIVMIIFIRRALARRAENESYMDAVLDGSVLRLRPKLM
                     TVAVTVLSLLPVMLSSGLGMEIMKPIATPTIGGMLTSTIYVLFLIPCLFAIGEDFRRR
                     RRA"
     gene            4455..4850
                     /locus_tag="VFV99_10180"
     CDS             4455..4850
                     /locus_tag="VFV99_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059718.1"
                     /translation="MRFATLLSCVWLVAATSALAQPPEPDPESAFAPATTPDPGSLLP
                     GGPAKPLVLGYCDKCHGLAWIERSGGSLDGWTSRLYRMNRAGAMVPVEQIPVIAEYLA
                     QALPERPRPPEPPKKRSGPSFSSSPGESP"
     gene            4883..5263
                     /locus_tag="VFV99_10185"
     CDS             4883..5263
                     /locus_tag="VFV99_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX5059719.1"
                     /translation="MLAAVTMFGTACGQAPSSTAADAGNGLTISFKTVNEPAKGANKL
                     EAVVKQDGKPVTDATVAVTFRMPAMPNMNMPEMHSTTPLVHEKDGRYVGTGQLEMAGT
                     WNVTVTVERGGTQLGSSRFSLQAK"
BASE COUNT          977 a         1834 c         1867 g          989 t
ORIGIN      
        1 aggacaaggt caccaaggtt atccgcgtcg gaccggatcc gtggcgcctg tacatgtcgc
       61 atgacggcaa gtacggcatc accgcgaaca acggcgacag cacgatctcg atcatcgaca
      121 tcgcgaagaa cgccgtcgtc gccacctatc cggtaggccc ggatctcacg ggcatcaact
      181 tcgccgcagg caaagcgttc gtgatcagct cgacgaccgg cttcctctac gtgttcgaca
      241 tgagcacgct taagcccgcg ggccgcatca agatcggcaa caacctgttc ttgcagacgg
      301 cgacgaccga cacggcggat caaaagctgt atctcggcag cgcgacggac aactcggtct
      361 acatcatcga cggtgcgacc gaagccgtgg agcgcgtggc caacgtgggg cagttcccgt
      421 ggggcaccca catcatggac agcaaggaca actactgcca ttgatgaacg gcacggccgc
      481 aatacgcact ttccgtgcaa ccgcggtgca cggcggaagg agggcggccg tcctgcccct
      541 cgctagaata gcggggtagc cgccatgctc gagtcgaatg aagccgcagt cgtcgatggt
      601 gcattctcgc tcgttgttgc tcgcgggccg gctcgccggg ctcgcgttgc tcgccgtcgc
      661 ggccggggca gactcgtcgc cgagcgcgtt cgctcaggag ccgcaagcgc aggcttccga
      721 ctcgccgcct gatgccgccg ctccgccggc gcaagggtcc ggcgttaggg cggccacgcg
      781 cctcgtgcgg cgctcggcca cggccgtcac agaagagcag gcctcggagc tcacgctgac
      841 gctgacggtc gccgaatcgc gcccgattca gacttgggtc cggaccgcgg gcgtgctgga
      901 taccgcgggc aaggtcctga ccgcgcagtt gcggccggcg gaggccgagc tcgtcgaagt
      961 cggccagcgt gtgcgcacgt tctcggtcaa ctcgcgctcc cagatggtcc aggcgaaggt
     1021 cacgcgggtg acgcctcggc ccggcggtgc gaccgttgaa gcgaccctcg ccaccgctgt
     1081 ggcgaacaat ggggcgcgcc atctcatgga gatcgtgacc gagaaaggac cgttcctttc
     1141 ggtgccgaac gaggcgatca tcgaagaagg cgagtcccaa gtcgtgtacc tcaagctgcc
     1201 gagcggcgaa ttcgcgccgc gcacgattca taccggcatc accggcgagc tctacaccca
     1261 aatcgtcgac ggcctcgagg atggcgacga ggtcgtctcg atcggcagct tcttcgtcga
     1321 cgcggacaat aagctcaagg gcgggccgac cgagccatga atcccgcgtt gttcgaaggc
     1381 atcgttcacg cgcgctggct gatcgttcta ttgatcctgg ccggcgtcat cgcgagcgtg
     1441 ttcgcgatgc gcgccgcgcc gctcgacgcg atccccgata cgtccgatcc gcaggtgatt
     1501 gtctacgcga agtgggcgcg cagccccgag cttctcgagg agcaagtcac cgagccggtc
     1561 gtgaaggcgt tgatcggctc gccggaggtg cgctcgatcc gcgcgacgtc gcacatgggc
     1621 tattcgttca tctacgtgat cctgaggagc acgtcggacc gcgcagccgt gcgccagctg
     1681 atcgcggacc gcctcgcgac gatccggcag ctgctacccg ctgacgccac ggtatcgatc
     1741 gggcccaatg cgagcggcat cggctggatc taccagtacg cgctgatcga tcacgagaag
     1801 ctgcgcgacc cgcgtgaaat gcggctgctc aacgagacgc agctcaagcc cgcgctgcag
     1861 gcgcttccgg gcgtggccga agtggcttcg gtcggcgggt tggagaagca gtaccagctc
     1921 aagctatttc cgcctctgct agccgatgcg ggaatctcgc tgcgcgagct cgccgccgcg
     1981 ctgcgcgacg cgctcgaaga ggcgggcggc cgcacgatcg aggtgacgaa ccgcgattat
     2041 caagttcgcg gcgtcgtgaa ccacgacaac atcgacgatc tcgaggcgct gatcgtgggg
     2101 cacgctccgg acgggcgcgc gatcagggtc aaggacgtgg gctacatcca gatcgggtac
     2161 gaccagcgcc gcgggatcgc cgatttgaac ggcgatggcg aagtggtcgg cggcatcgtg
     2221 atcatggagc agtcgcagaa tgcgctcgag atcacggccg cgctcaagaa gcggctcgcc
     2281 gaagttgcgg catcgctgcc gcccggcatc gagatcgcga ccacctatga ccgctcgcag
     2341 ctgatcttgg gcacgcttcg acacttcttc ctcacgctgg cgtacgagct cggtgtcgtg
     2401 atcctcgtga cggcgctgtt tctgcgcaac gctcggacct cgctcgcgcc cgtcgcaatc
     2461 ctgctgctcg gcgtgctgtt cacggcgatc ccgatggcgc tgctgagcca aaccatcaac
     2521 ctgttctcgc tcgcggggct cttcatcgca atcggcgaga tggtcgatgc gaccatcgtc
     2581 atcgtcgaga acagcaccgc ggagcttgcc gcgcatcccg gcgcgagcga gaacgagaag
     2641 cggcagatcg tcctgcgctc gatcgcgggt gtcgcgaagc cgttgctgtt ctcgttgctg
     2701 atcatcctcg cgtcgttcct gccggtgttc ttcctcgacg acaaggaagc gcggatgttc
     2761 gatccgctcg cgtacagcaa aacgttcgcg atggcgttct cgaccctgct cacgctgtgc
     2821 gttttgccga tcgcgatgct gtgggtgttc aagggccaag tgattcacgg gccttcgcgg
     2881 ttcgagctcg gcttcacgaa ttggtatcgg cgggcgacgc agtggttcgt gcgttaccgc
     2941 tatgcgttcc tcgcggtcaa cgtcgtcctg ctcgttgcgg ccgtggtgct cttgcagggc
     3001 tttcgccggg acttcatgcc gcgcatggag gagggctcga tcctttacat gccgacgacg
     3061 ttgcccggcg tgccgatgaa agaggcgggc tgggcgctgc agcagatgga caagaagctc
     3121 gccgcgtttc ccgaggtcgc gagcgtgttc ggcaagctcg gccgcgcgga cacggcgacc
     3181 gacgcggcgc cggtctcgat gatcgaggcg acgatccaac tgaaggaccg agagcactgg
     3241 cggccgaatc tcacgaagga ccagctcgtc gccgagatgg acgccgcgat gcaggtcgtg
     3301 ggctacgcga actcgtgggt gcagccgatc aacgcgcgca cggtcatgca agacacgggc
     3361 atccagaccc cgaccggaat caaggtgaag gggcccgacg tcgcgacgat cgagagcatc
     3421 ggcgaagaga tcgagcagct gctgcgtcct tatcccggca cgcagtccgt catcgcggaa
     3481 cgcatctcgt cgggttactt catcgacgtc gagttcgacg cacagcggtt ggccgctgcg
     3541 gggctctccg cgcaggaggc catgcccgtc gtgcgctttg gcgtcggcgg cgacaacctc
     3601 ttagcgatca aagactcgag cggcgcccag ataccgctga gcctgcagta ctcgcccgag
     3661 tacctcgaca cgctcgagaa agttcgtacg tcgccggtcg tcgggcccga cggcacgacc
     3721 gttgcgctcg gcgagatcgc ggacgtcgcg gtgaagaaaa tgcccgacat gctgcgcaac
     3781 gacaacggca cgctcgcagg ttatgtgtac gtctacttga acggcgacgt caccgccacg
     3841 gattacgtcg cgggcgcgca ggcgttcctc gccgagaacc tcgcgctgcc gagcggctac
     3901 tcgatcgaat ggaccggtga ttaccagaat gcgctcgcgg ccgccaaacg gctcgagatc
     3961 gtgattccaa cgacgctcgt cgtgatcttc gtgttgctcg tgctcgcgtt tcgctcgatc
     4021 gcggacagcc tgctgatcat gttgtccgtg ccgttcgcgc tgaccggcgg ggtgttcttg
     4081 cagtggctgc tcggctaccc gatgaccacg gcggtgatcg tcggctacat cgcgctgttc
     4141 gcggttgcga ttcagaccgg catcgtcatg atcatcttca tccggcgcgc gctcgcacgg
     4201 cgagccgaga acgagtccta catggacgcg gtgctcgacg gctcggtgtt gcggctgcgc
     4261 ccgaagctca tgaccgtcgc ggtgacggtg ttgtctctgt tgccggtgat gttgtcgagc
     4321 gggctcggaa tggaaatcat gaagccgatc gcgacgccga cgatcggcgg aatgctcacg
     4381 tcgacgatct acgtgctgtt cctgattccg tgcctgttcg cgatcggcga ggatttccgg
     4441 cggagacgac gcgcgtgagg ttcgcgacgt tgctgtcctg cgtttggctc gtcgcggcca
     4501 cgagcgcact cgcgcagccg cccgagccgg atcccgagtc ggcgttcgcg cccgccacga
     4561 ctcccgatcc gggctctctt ctgccgggtg ggcccgcgaa gcccctggtt ctcggctact
     4621 gcgacaagtg ccacggcctc gcatggatcg agcggtccgg cggctcgctc gacggctgga
     4681 cgtcgcgcct ctaccgcatg aaccgtgcag gtgcgatggt gccggtcgag cagattccgg
     4741 tcattgcgga atacctcgcg caggcgcttc cggaacggcc acgaccgccc gagccgccga
     4801 agaagcgctc gggcccttcc ttctcgagct caccaggaga atccccatga gccacgcaac
     4861 ccgttcgtcc aggatgcttg cgatgctggc ggcggtcacg atgttcggaa cggcgtgcgg
     4921 ccaggcgccg tcgagcacgg cggccgacgc cgggaatggc ttgacgatca gcttcaagac
     4981 ggtcaacgag cccgccaagg gcgcgaacaa gctcgaagcc gtcgtgaagc aggacggcaa
     5041 gcccgtgacc gatgccacgg tcgcggtgac gtttcgcatg cccgccatgc cgaacatgaa
     5101 catgcccgag atgcacagca cgacgccgct cgtgcacgag aaggacggcc gctacgttgg
     5161 cacggggcag ctcgagatgg cgggcacttg gaacgtgacc gtaaccgtcg agcgcggcgg
     5221 cacgcagctc ggtagctcac ggttcagcct gcaagcgaag taactgcccg tgctataacg
     5281 agccggcgtg tcgagtgacg aaacgatcga cgctcgccgg agtcgtcgcg gccgcggtac
     5341 tcatcatgag tgcgcaaggc gtgagcgccg caacgacgga cgcagcggtg cgcaacaagc
     5401 cgatcgcccg ggcaaacagg atcaagatta gcgcttgccc gcagctgcca cgcggcgctg
     5461 gcttcccttt gctttgatca cgtctttgct ctagggtcgg tccaataagt ccgatcctag
     5521 ctcgggagga aatgatgagc cgcttcgcga ttgccgccgg ccttctcgcc gcgtcgatcg
     5581 gcgcgtcggc cacgtgggct cagcccggcg ccttcaaggc gccgcgtact tcgttcggcc
     5641 atcccgatct gtcgggcact tggacga
//
LOCUS       DASUFX010000255         9089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_456328,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000255 DASUFX010000000
VERSION     DASUFX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456328"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /gene="dctP"
                     /locus_tag="VFV99_10190"
     CDS             complement(<1..621)
                     /gene="dctP"
                     /locus_tag="VFV99_10190"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5059720.1"
                     /translation="MSRFAVAVVMLAWLVPTHHAAADEPKHKLRISTAAPDGTSWANT
                     FIAFGRDIAKQTHGEVELKYYFGGIAGDEMEVYDLMQAGKLDGIVSGGPLCRKVAPSM
                     RVLDVIGLFQNQEEAQHVLNQLRPTLTKEAHDNGYALLATGSLGPIIMFSREPITSMA
                     DLQKSRLWTWDLNTVFIKEGTEMKIPIVGTGLTQAQKAFDEKRTDGF"
     gene            complement(642..2357)
                     /locus_tag="VFV99_10195"
     CDS             complement(642..2357)
                     /locus_tag="VFV99_10195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059721.1"
                     /translation="MSRLETLPDGIIEKLRRKSFVNGEIIASRFRLKSLLGTGAMGQV
                     FVAENLATNFEVAVKLLKADLIASSEFRQRFQKEAEAVGSISHQNVARFFDLVVGDPT
                     FLVMEYVPGPTLQQMIEQERLPYERAVRIAVRLCWGLEAAHQRGIVHRDLKPANIIVA
                     TDPEVGDQPKIIDFGLAKLATATETAQLTRTGQIVGTPEYMAPEQIEGKTIDARADVY
                     ALGCVLYKMIAGKTPFGGDDDVQILYRQLHQKPTPLSEHVPEVPPKLDEVLARALAKK
                     PADRFENTGELARALSRTFDRRRTSRISAESIAEQKKPPIALVAATTLAVGAVAGVLL
                     GRLPSHGDGKPSQPAAVAGSATPAAGNELLVIDTTPEGATVELDGKALTEVTPTAVRG
                     LAPGPHTVKLTAKGRSAVERKLTIERDARASLVVNLPPSQRGVLVRSVPGGAAVYLDN
                     DPVGIAPVTVTVDEEDFHELRLEKLGFAPLIRAIKPGDEEAELALTMELDAQPHSELQ
                     VDSSADAEIWIDGVDTMLETPAMGIRLAPGRHTIQLRDSTGAASKVTTIDVKPGETLR
                     MTINL"
     gene            complement(2639..3118)
                     /locus_tag="VFV99_10200"
     CDS             complement(2639..3118)
                     /locus_tag="VFV99_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059722.1"
                     /translation="MTDHKPLLVVPATTNLARAFDDDLASLRVSERASRNGVVVVAPR
                     ETQPHELMAACRLWPAVLGELGITRVAVVLSVQAYGTAEPLLRACVRCLKASGIAVAY
                     FHEGHLESDHIAAWFEQRKARRRITSDSMIKLSERYAERGDARAAYHAIVIAEQLAG"
     gene            complement(3155..3979)
                     /locus_tag="VFV99_10205"
     CDS             complement(3155..3979)
                     /locus_tag="VFV99_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059723.1"
                     /translation="MFDRGRHDVTFGQRIRSERLFGNAMAAGACAGAVAIANLVAPGL
                     AWAAVAAFPVLVAAREFVDQNRTIARWWRGARATLPTTSGEVIVRGIARALDANAVAP
                     YSRRECLGYWAQFTGTHFGDEGGVREDRYVAEDHVQLADVLVEVGDAKILVPADRARL
                     ICDPRSGEGARLRVAPNHGAEVDVFVPMTKELLGRARHQEHVLRAGDEVVVVGRLEAI
                     GGDPYRDAIGATHRVAGERKAALVLRPPELAPDARYDAPTLAERPRVEARPYTRPR"
     gene            complement(3999..4655)
                     /locus_tag="VFV99_10210"
     CDS             complement(3999..4655)
                     /locus_tag="VFV99_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059724.1"
                     /translation="MFQLAHRNQRQRDVDVSHKKAIEPASSTVGKRSLTQEMSFGAPD
                     RYTRAIESARRELRAVRTVALPQYLRTLRDNGLEAIAKQTQLATLAMQVRHLLMTANQ
                     HVKALEKAAVYRDPMVLFLRHDLDTLAGRAAKLGVYRGAENMMPKAHVEEEKKPQSRG
                     DNAQLARKSTKTRHALRVNLQAPVERKRKPAPVPQHAPTRAMRGSSTSVRALQRKAHG
                     "
     gene            4751..5725
                     /locus_tag="VFV99_10215"
     CDS             4751..5725
                     /locus_tag="VFV99_10215"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX5059725.1"
                     /translation="MERIVHICNGDSTADTLSLADLPGEIRVWADALDLGPVLPVSDQ
                     EHYKRRGDFWSSKGYGSAADAAQRIADYDKNFDEAASADELILWYEHDLFDQLALIRL
                     LARLARRGLPQTLTIVSIDRHPEIPNFLGLGQLKPEQLAELWPRRTPLSRDAIDEAVS
                     AWIAVTANDPRALPFLTKRVKALPFLGGALERQLEEFPDVTSGLSRTERQTLAAIARG
                     EGTGGALMQASHAIDPRYPITDGLLLDILKRFGAVGFIDGAPTATPGADALKSINVTV
                     TPLGRQALAGAIDRVHECGIDEWRGGVHLTGKGPVWRWHSSDRKLIER"
     gene            complement(5726..6109)
                     /locus_tag="VFV99_10220"
     CDS             complement(5726..6109)
                     /locus_tag="VFV99_10220"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX5059726.1"
                     /translation="MEKPTSFEEFWPYYVSQHRNKTCRQLHFVGTSIAMGCLAVSPFY
                     PPAALAAPVAGYGLAWIGHFVFEKNKPASWMGPKEFVWSLRGDLRMWRYMVAGMMDAE
                     VERVGGGSVESMPSTNGTTTTETFA"
     gene            complement(6128..7567)
                     /locus_tag="VFV99_10225"
     CDS             complement(6128..7567)
                     /locus_tag="VFV99_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059727.1"
                     /translation="MRRDTAFMLHILFVLMVTTSTARGRGIVLESYTGDRPADASRLL
                     APVLEELSRRDYDAGDSVARLFEAQSSKAAMSTNGLPGDFSAQVDKGFGEWVGGRFTE
                     ATKILVPLVEAARANSGTIAKDPSLRGPYLKALIALALAYQRIGDLSSMRTTFEDVVR
                     SFPDAQVSRATYGPDAAKAFEQTRSDLQAKGKGKLTVKCDDTAVVFVNETYRAAGATT
                     IELPPGEYRVIVMMNKQPSRNHRVMVRPNAESTVEIDAKLDQAIHTSGYTGLRFANEA
                     DREDHEAPFAQQFAKMVGASAVAVVGIDSVHGHGAIVGSLVSLQTGKEIRRASIPVEP
                     DPSTDKLRSLARFLAGDDPAPGLEVQFSGAIQDKSDGGGHHDDGETPSGDGGGRWGGW
                     RYITAGLGVGGLAAGVVLLVMDGKATMDPPPGQQPNDFYDTAVPGYIALAGGAVFTGV
                     SIYLFATHKSAPVVAPTQGGATVGFATHW"
     gene            complement(7564..8085)
                     /locus_tag="VFV99_10230"
     CDS             complement(7564..8085)
                     /locus_tag="VFV99_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059728.1"
                     /translation="MWPPAVVRFGALALVAMSCWSGKSPDSTLDNRALSGERDMTAAY
                     YCSIQDGDFKYPAFPCAVRKIEGRWMLAKLAGSQRFRGEVTPRGEGFSFDGELYCPWG
                     DCTQPLHGVFKSIGNGALRGVFRDASMTVVLKPAPDAAFGGATYGGDGYGGFGYGGAT
                     YGLPAKQRGNRRP"
     gene            complement(8125..8883)
                     /locus_tag="VFV99_10235"
     CDS             complement(8125..8883)
                     /locus_tag="VFV99_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059729.1"
                     /translation="MRTPHLAALLVVLLTGCLAGEISDVGGDDQGSGSGSGSGSGSGS
                     GSNTSTPRVDATLDKTSVMSELGMTENLVLTLTSANDFAGTVTVTPKLMDGTTEVMGW
                     VTASAPTIDLTAGGTATVMLAVKIPTDAASLGPTLKIEATSSAAPALAESAFAIANKY
                     TIVIPANTGTGTHTGLPGINTTLRLRMGAQIVFHNADGITHVIHGDGGIPHENIQNGG
                     GAGTDYTVTPDGNATWYCHSHEGSNQARPVAVAP"
BASE COUNT         1469 a         3130 c         2894 g         1596 t
ORIGIN      
        1 gaagccgtcg gtgcgttttt catcgaacgc cttctgggcc tgggtgagac cggtgccgac
       61 gatcggaatc ttcatctcgg tgccttcctt gatgaagacc gtattgagat cccacgtcca
      121 cagccgggac ttttggagat ccgccatgct cgtgatcggc tcgcgcgaga acatgatgat
      181 cgggcccagg ctgccggtcg cgagcagcgc gtagccgttg tcgtgcgcct ctttcgtgag
      241 cgtcggccgc aactggttga gcacgtgctg tgcttcctct tggttctgga agaggccaat
      301 gacatcgagc acgcgcatcg acggcgcgac cttgcggcag agcggcccgc cggacacgat
      361 gccgtcgagc ttgccggctt gcatgaggtc gtagacctcc atctcatcac cggcgattcc
      421 accgaagtag tacttgagct cgacctcgcc gtgcgtctgt ttcgcgatgt cgcgtccgaa
      481 tgcgatgaac gtattcgccc agctcgtacc gtccggcgct gcggtcgaga tgcgcagctt
      541 gtgctttggc tcgtcggcgg ccgcgtggtg cgtaggcacg agccaggcga gcatcacgac
      601 tgcaacggcg aaccgggaca tgcgcgtcat tcgtctcccc ctcacagatt gatcgtcatt
      661 cgtaacgtct cacctggctt gacgtcgatc gtggtcacct tgctggccgc cccggtcgag
      721 tcgcgaagct ggatcgtgtg tcggccgggc gcgagtcgga tgcccatcgc cggcgtctcg
      781 agcatggtgt cgacgccatc gatccagatc tctgcatccg ccgacgaatc gacctggagc
      841 tccgagtgcg gttgcgcgtc gagctccatc gtgagcgcga gctcggcttc ctcgtcgccg
      901 ggcttgatcg cccggataag tggcgcgaag ccgagcttct cgagacgcag ctcgtgaaag
      961 tcttcttcgt cgaccgtgac ggtgacgggt gcaatgccga ccgggtcgtt gtcgaggtac
     1021 acggcggcgc cgccggggac gctgcgcacg agcacgccgc gctgcgaggg cggcagattc
     1081 acgacgagcg atgcacgtgc gtcgcgttcg atggtcagct tgcgctcgac cgcgctacgc
     1141 cccttcgcgg tgagtttcac cgtgtgcggg ccgggcgcga ggccgcgaac cgctgtgggc
     1201 gtgacttcgg tgagtgcctt gccgtcgagc tcgacggtcg caccttccgg cgtcgtatcg
     1261 atgaccagca gctcgttgcc tgccgccggt gtggcgctcc cagcgaccgc ggcgggttgc
     1321 gacggtttgc catcaccgtg tgacggcagg cggccgagca gcacgccagc caccgcgcca
     1381 accgcgagtg tggtcgcagc gacgagcgcg atcggcggct tcttctgttc cgcgatcgat
     1441 tccgcggaga tccgcgacgt gcgccggcga tcgaacgtgc gggacaatgc ccgtgcaagc
     1501 tcgccggtgt tttcgaagcg atcggccggc ttcttcgcga gcgcccgcgc gagaacttcg
     1561 tcgagcttcg gtggcacctc ggggacatgc tcgctgagcg gcgtcggctt ctggtgaagc
     1621 tggcggtaga gaatctgcac gtcgtcgtcg ccgccaaacg gcgtcttgcc cgcgatcatc
     1681 ttgtagagca cgcagccgag cgcgtagacg tcggcacgcg catcgatggt cttgccctcg
     1741 atctgctcgg gcgccatgta ctcgggcgtg ccgacgatct ggccggtgcg cgtgagctgc
     1801 gcggtctcgg tcgccgtggc gagcttcgcg aggccgaagt cgatgatctt gggctggtcg
     1861 ccgacttccg gatccgttgc gacgatgatg ttcgcgggct tgagatcgcg gtgcacgatg
     1921 ccgcgctggt gcgccgcttc gaggccccag cacagacgta ccgcgatcct cacggcgcgc
     1981 tcgtacggca accgttcctg ctcgatcatc tgctggagcg tcggccccgg tacgtactcc
     2041 atgaccagga atgtcggatc gccgacgacg aggtcgaaga aacgcgccac gttctggtgg
     2101 ctgatcgagc cgacggcttc ggcctccttc tgaaagcgct ggcgaaactc cgagctcgcg
     2161 atgaggtcgg ccttgagcag cttcacggca acttcgaagt tcgtcgcaag gttttcggcg
     2221 acgaacacct gccccatcgc gcccgtcccc agcagcgact tcaaacgaaa gcgactggcg
     2281 atgatctcgc cgttcacgaa cgacttgcgc cgaagcttct cgatgatgcc gtccggaagt
     2341 gtttccagac gtgacattcc cgatgccacg tacgcgccgc ttccagcccc aacgttcttc
     2401 tcgcttagcc ccatcgcctg gcgtgcatcg ctatccccac cgaggacacg catacccagg
     2461 atatcagcct cggggatcac cctggaatga tttggccgca aacttttcgt gtgggcagag
     2521 cgcgccgggc tgcgcccttg tcgtcgaacc gcagctaaca gttcccgaac gagaggtttg
     2581 acctgcaacg attggctgca ggctcacagt tcgccagtcg ctcgccggaa cgccgcgcct
     2641 acccggcgag ttgctcggcg atcacgatcg cgtgatacgc cgcgcgtgca tccccgcgct
     2701 ccgcgtagcg ctcgctcaac ttgatcatcg agtcgctcgt gatccgacga cgcgccttcc
     2761 gctgctcgaa ccacgccgcg atgtgatcgc tctcgagatg gccctcgtga aagtacgcga
     2821 ccgcgatgcc actcgccttc aagcaccgga cgcacgcgcg aagcagcggc tcggcggtcc
     2881 cgtacgcctg cacgctgagc acgacggcga cccgggtgat gcccagctcg ccgagcaccg
     2941 ccggccacag ccgacacgcc gccatcagct cgtgtggctg cgtctcacgc ggtgcgacca
     3001 cgacgacgcc gttccgcgac gcccgctccg acacccgcag cgacgcgagg tcgtcatcga
     3061 acgcgcgcgc gaggttggtc gtagccggca cgacgagcaa tggcttgtga tccgtcatgg
     3121 gagcgcctat cggccgcccc acacgtcggt ttcgtcagcg cggacgcgtg taaggtcgcg
     3181 cctctacacg cggtcgctcg gcgagggtcg gcgcatcgta acgcgcgtcg ggagccagct
     3241 ccggcggccg taggacgagg gccgccttcc gttcaccggc gacgcggtgt gtggccccga
     3301 tcgcatcgcg atacggatcg ccgccgatcg cctccagccg gccgaccacc acgacctcgt
     3361 cgccggcgcg tagcacgtgc tcctgatgac gcgcgcgacc gagcagctcc ttcgtcatcg
     3421 gcacgaagac gtcgacctcg gctccatgat tcggcgccac ccgcaatcgc gctccctcgc
     3481 cgcttcgcgg atcgcagatc agccgcgcgc gatcggcagg cacgaggatc ttcgcatcgc
     3541 cgacctcgac cagcacgtcg gcgagctgca cgtggtcctc ggcgacgtag cgatcctcgc
     3601 gcacgccgcc ctcgtcgccg aagtgtgtgc cggtaaactg cgcccagtag ccgaggcact
     3661 cgcggcgcga gtacggcgcg acggcgttcg catcgagcgc gcgcgcaatg ccgcgaacga
     3721 tcacctcacc actcgttgtc ggcagcgtcg cgcgcgcgcc gcgccaccac cgcgcgatcg
     3781 tacggttctg gtcgacgaat tcgcgcgcag ccacgagcac cggaaacgcc gcgaccgcgg
     3841 cccaggcgag accgggcgcg acgagattcg cgattgccac cgcgccggcg cacgcgcccg
     3901 ctgccatcgc gttgccgaac aaccgctcgg agcgaatgcg ctgaccgaac gtgacgtcgt
     3961 ggcggccgcg atcgaacatc gcgcgcacag catagcgatc atccgtgcgc cttgcgctgc
     4021 agcgcgcgca ccgaggtcga gctgccacgc attgcacgtg tcggtgcgtg ttgcggcacc
     4081 ggagccggct tgcgcttgcg ttcgaccggc gcctgcaagt tcacccgcag cgcgtgccgc
     4141 gtcttcgtcg acttgcgcgc gagctgggcg ttatcgccgc gcgactgcgg cttcttctcc
     4201 tcctcgacgt gcgccttcgg catcatgttc tcggcgccgc ggtacacgcc gagcttggca
     4261 gcgcggccgg cgagcgtgtc gagatcgtgg cggaggaaca gcaccatcgg atcgcgatac
     4321 acggccgcct tctcgagtgc cttgacgtgc tggttcgccg tcatcagcag gtggcgcacc
     4381 tgcatcgcga gcgtcgcgag ctgcgtttgt ttcgcgattg cttcgaggcc gttgtcgcgc
     4441 agtgttcgca ggtactgcgg cagtgcaacc gtgcgcaccg cgcgcagctc gcgccgcgcc
     4501 gactcgatcg cgcgcgtgta gcgatcaggt gcgccgaaac tcatctcctg cgtcagcgag
     4561 cgcttgccta ccgtcgagct cgcaggctcg atcgctttct tgtggctgac atcgacgtcc
     4621 cgctggcgct gattgcgatg ggcgagctga aacacgccgc ctgtatcgac ggcgacctcg
     4681 cgcggttgcg cccgtcgatc ggcccggcag aacggggact taccgggtcc gtgcgtgcta
     4741 cgcccatgcc atggaacgga tcgtccacat ctgcaacggc gactcgacgg cagacacgct
     4801 ctcgcttgcc gatctaccgg gcgagatccg cgtgtgggcc gatgcgctcg acctcggccc
     4861 ggtgttgccg gtcagcgatc aggaacacta caagcgcaga ggcgatttct ggtcgagcaa
     4921 aggctacggc agcgccgccg atgccgcgca gcgaatcgcc gactacgaca agaacttcga
     4981 cgaggcggcg agcgccgacg agctcatcct ctggtacgag catgatctgt tcgatcagct
     5041 cgcgctgatc cgtctgctcg cacggcttgc gcgtcgcggt ctgccgcaga cgctgacgat
     5101 cgtctcgatc gatcggcatc ccgaaatccc gaacttcctc ggtcttggtc agctcaagcc
     5161 cgagcagctc gccgagctgt ggccacgccg cacgccattg tcgcgcgatg ccatcgacga
     5221 ggcggtcagc gcatggatcg cggtcacggc gaacgatccg cgcgccctgc cgttcctcac
     5281 caagcgcgtc aaggcgctgc cgttcctcgg tggcgcgctc gagcgtcagc tcgaagagtt
     5341 cccggatgta acgagcggcc tctcgcgcac cgagcggcaa acgcttgccg cgatcgcgcg
     5401 tggcgaaggc accggcggcg cgctgatgca ggcatcgcac gcgatcgatc cacgctatcc
     5461 gatcaccgac ggtttgctcc tcgacatcct caagcgcttt ggcgccgtcg gcttcatcga
     5521 cggtgcaccg acggccacgc ctggcgccga cgcactcaaa tcgatcaacg tcaccgtgac
     5581 accactcggt cgccaggcgc tcgcaggcgc gatcgatcgc gtccacgaat gcggtatcga
     5641 cgagtggcgc ggcggcgttc acctcaccgg caagggcccg gtgtggcgct ggcactccag
     5701 cgaccgcaaa ttgatcgagc gctgattacg cgaacgtctc ggtcgtcgtc gtgccgttcg
     5761 tgctcggcat cgactccaca ctaccgccac cgacgcgctc gacttccgcg tccatcatgc
     5821 ccgcgaccat gtagcgccac atgcgaaggt cgccgcgcag cgaccagacg aattccttcg
     5881 gacccatcca cgacgcgggc ttgttcttct cgaacacgaa gtggccgatc cacgcgaggc
     5941 cgtaacccgc gacaggcgcc gcgagcgcag ccggcggata gaacggcgag accgcgaggc
     6001 agcccatcgc gatcgacgtg ccaacgaagt gcagctgccg gcacgtcttg ttgcggtgct
     6061 gcgagacgta atagggccag aactcttcga agctcgtcgg cttttccatg cgccggactg
     6121 taacgcgcta ccagtgcgtc gcgaagccca cagttgcgcc gccctgagtc ggggccacga
     6181 ccggcgcgct cttgtgcgtc gcaaacaggt agatcgacac gcccgtgaac accgctccgc
     6241 cagcgagcgc gatgtacccg ggcaccgccg tatcgtagaa gtcgttgggc tgctgaccag
     6301 gtggcgggtc catcgtggcc ttcccgtcca tgacgaggag cacgacgcca gccgcaagcc
     6361 cgccaacgcc aagacccgcg gtgatgtacc gccagccgcc ccaacgtccg ccaccatcgc
     6421 cgctcggcgt ctcgccatcg tcatggtgac caccgccgtc ggacttgtct tgtatcgcgc
     6481 ccgagaactg cacctcgagg cccggcgctg gatcatcgcc cgcgagaaac cgcgcgagcg
     6541 agcgcagctt gtccgtggac ggatcgggct cgaccggaat gctcgcgcgc cgaatctcct
     6601 tgccggtctg caagctgacg agagagccca cgatagcgcc gtggccgtgc accgagtcga
     6661 tgccgacgac tgcgaccgcg ctcgcgccga ccatcttcgc gaactgctgc gcgaacggcg
     6721 cctcgtgatc ctcgcgatcg gcttcgttcg caaaccgaag cccggtgtag ccgctcgtgt
     6781 ggatcgcctg atcgagcttc gcgtcgatct cgaccgtcga ctccgcattc gggcgcacca
     6841 tcacgcggtg attgcgcgac ggctgcttgt tcatcatcac gatcacgcgg tactcgccgg
     6901 gcggtagctc gatcgtcgtc gcacctgccg cgcgatacgt ctcgttcacg aacacgacgg
     6961 cggtgtcgtc gcacttgacc gtcagcttgc ctttgccctt cgcctgcaga tcgctccgcg
     7021 tctgctcgaa cgccttcgcg gcatccggtc cgtacgtcgc gcgtgacacc tgcgcgtcgg
     7081 ggaagctgcg cacgacgtcc tcgaacgtcg tacgcatcga gctcagatca ccgatacgtt
     7141 gatacgcgag cgcgagcgcg atcagtgcct tcaagtacgg accgcgcagc gacggatcct
     7201 tcgcgatggt gcccgaattc gccctcgccg cttcgacgag cggcacgaga atcttcgtcg
     7261 cctcggtgaa gcgaccgccg acccactcgc caaatccttt gtcgacctgt gccgagaagt
     7321 cgcccggcaa gccattcgtg ctcatcgcgg ccttcgacga ctgcgcctcg aacaagcgcg
     7381 cgacgctgtc gccggcgtcg taatcgcggc gcgacagctc ctcgaggacg ggcgcgagca
     7441 agcgcgacgc atcggcgggc cggtcgccgg tataggactc gagcacgatg ccacggccgc
     7501 gcgcggtcga ggtcgtcacc atcagaacga acagaatgtg gagcatgaag gctgtatcgc
     7561 gcctcatggt cgtcggttgc ctctctgttt cgcgggcagg ccgtaggtgg cgccgccgta
     7621 gccaaaaccg ccatatccat cgccgccata cgtcgcaccg ccgaatgccg catcaggtgc
     7681 aggtttgagc acgaccgtca tgctcgcatc gcgaaatacg ccgcgcaacg cgccgttgcc
     7741 gatcgacttg aacacgccgt gcagcggttg cgtgcaatcg ccccacgggc agtagagctc
     7801 gccgtcgaag ctgaagccct caccgcgcgg cgtcacctcg ccgcgaaagc gctgcgagcc
     7861 ggcgagcttc gcgagcatcc agcggccctc gatcttgcgc accgcgcacg gaaatgccgg
     7921 gtacttgaag tcgccatcct ggatcgagca gtagtacgcg gccgtcatgt cgcgctcgcc
     7981 gctcagcgca cgattgtcga gcgtgctgtc cggcgacttg ccgctccagc acgacatcgc
     8041 gacgagcgcg agggcgccga aacgaacaac ggcaggcggc cacatggggc gagcctgccg
     8101 gacgctgcgt cgagatgcaa ccgactaagg cgcaaccgcg accgggcgcg cctggttcga
     8161 accctcgtgg ctgtggcagt accacgttgc attgccgtcg ggcgtcaccg tgtaatcggt
     8221 gccggcgccg ccgccgttct ggatgttctc gtgtggaatg ccgccatcgc cgtgaatcac
     8281 gtgcgtgatg ccatcagcgt tgtggaacac gatctgtgcc cccatgcgga ggcgaagcgt
     8341 cgtgttgata cccggaagtc ccgtgtgtgt ccccgtgccc gtgtttgcgg ggatcacgat
     8401 ggtgtacttg ttcgcgattg cgaatgccga ttcggccaat gccggagcgg cgctgctggt
     8461 ggcttcgatc ttgagcgtcg ggccgagcga agcggcatcg gtcgggatct tcaccgcgag
     8521 catcaccgtc gcagtgccac ccgcggtcag gtcgatcgtc ggcgcgctcg cggtcaccca
     8581 acccatcacc tcggtcgttc cgtccatcag cttcggcgtg accgtcaccg tgcccgcgaa
     8641 gtcattcgca ctcgtcagcg tcagaacgag gttctcggtc atgccgagct cgctcatgac
     8701 cgaggtctta tcgagcgtcg catccacccg tggggtcgac gtgttgctgc cactgccgct
     8761 cccactccca ctccccgaac ccgacccgga gccctgatca tcgccgccca cgtctgaaat
     8821 ctcacccgcc aaacagccgg tgaggaggac aacaagaagg gcagcaaggt ggggtgtacg
     8881 catgttgcgc agtatgagca aacccaatgc cggcagtaac ttgctgttta cgctgggggg
     8941 cactcaccca gtgatgggag ttcggacccc aggtgggaac gactttcccc ggggatcgcg
     9001 gttttcctcc aacgaccgcg cggagatgag agcgcaggtt ccgtggtccc gctgactctc
     9061 gatgcagctc ccgctgactc tcgatgcag
//
LOCUS       DASUFX010000256         9514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1845083, whole genome shotgun sequence.
ACCESSION   DASUFX010000256 DASUFX010000000
VERSION     DASUFX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845083"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1169)
                     /locus_tag="VFV99_10240"
     CDS             complement(63..1169)
                     /locus_tag="VFV99_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5059730.1"
                     /translation="MAKFDLWPLAAAATAALTLGACMDEPRSADPAAPDTSALDSSAT
                     DDVQLDHDALIALGKEHVRAMRAAGEISDEPARVMTLAPTEPVVPPECGLLPVIFGTA
                     GDDVIVGTNEAECIVALEGDDVVFAGGGDDVVIAGAGHDVVDGDSGEDTIYGNDGADV
                     IFGDVGADTIYGGDGPDLLFAGGGDDTVDGGAGIDSIDGSSGADNLYGGAGADHIDGG
                     LGPDSIDGGAGNDTISGGGGADAITGGEGDDTIDADEGQDFADGGGGNDTINGGVGPD
                     TLIGGDGNDTIDGEGGDDVVVGGAGNDILKGNTGNDTLTGGPGDDNLDGGPGDDNLFG
                     GAGNDVLTQGGGMGTVDGGPGTDTCSVPNTNCEP"
     gene            complement(1459..2760)
                     /locus_tag="VFV99_10245"
     CDS             complement(1459..2760)
                     /locus_tag="VFV99_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059731.1"
                     /translation="MVNRVAACLGMLALAGCDYLTSSFDTNDSSGDQFPIFVDNASGA
                     VVIGMQEAGDLDQRQAVLDVMSPMTVIDRGPVVPASMETTDVTILGAPVAGDDLTLPR
                     AKVDDLQVTTLHPCDETVRDCTVGTETAPRPFNAVLGLNAFTGDALRLALGSDQIFIL
                     PDVAGSDLRRARSCDSVMSSPFSGGGTLVIGGTEIGFPGLRIAIDSCMLPRPINRFPN
                     GVPATQAQRGVDALFVLSTGVGTSLLSRSAYQHLRELDSSLPAFDALPEATVFLPSGP
                     VVGNVTTLASIALVSNSGSNPRAPCRQMWASHLLALQDCLPGDDCPCQLGETFCGVPA
                     VIELAPLAGIPLLIVPDTNPTLQSLRAEIRPDRPEVDGILGADAIEALELDIDFAHNR
                     LLGRCTDRAVCGARVALPNKEARSYLNGCLDGMPGPIVYEP"
     gene            complement(2754..3515)
                     /locus_tag="VFV99_10250"
     CDS             complement(2754..3515)
                     /locus_tag="VFV99_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059732.1"
                     /translation="MKIDRSNWGRLRITGGDRERFLQGLTTINVQSLADGANGWGAIL
                     SPKGRVLTVFNLAKLGQEFLLACEPALAEKTRALLERYAVMDDVGFEPITGPAHQTWE
                     EPVSVWRAPIAEGAGDVAETDDAAERMRIRGGFMRYDVDVDEDHFPFETPLAQFLDYG
                     KGCYVGQEPVFRVHAQGNSARVLRGFVIEGDTPVARGTVIKHANKDKAGEVTSAIAAG
                     DGTTIALGYLHRTVWDVGGTVEIDGRKATVRELPW"
     gene            complement(3512..3988)
                     /locus_tag="VFV99_10255"
     CDS             complement(3512..3988)
                     /locus_tag="VFV99_10255"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5059733.1"
                     /translation="MTPEQTRCVMTQIVMPMHTNGVAGVMFGGIMMQWIDVCAGVAAM
                     RHASGAVLTASIDRLDFLSPVRVGEIVVLQAMVNYTARTSMEVGCRVETEDMTTGQRR
                     YVTKAYLTFVAIDAMGRPRPIPPLELVTEDDHRRHADAEFRRAERLKAAGRAKGSS"
     gene            complement(4152..7526)
                     /locus_tag="VFV99_10260"
     CDS             complement(4152..7526)
                     /locus_tag="VFV99_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX5059734.1"
                     /translation="MKRALVLMLVVASAPALAQAPRAPAPAQSPTQPPPATEEDTLTT
                     QPKFDEAACLQLDLTPGAVLQEPPPPGELAPPIPWSEFDIQGQLIDRKKTVMALLDPT
                     MQQLRTSLSRATLPQVAGVLARFGYQLIGHVTKDIPNGTQLVLYVAPLPLVKKVKVKV
                     RFDWRRTFSFSGSLFDKQLGDDIVRRMSFRSGSYLPWDPPPTDVRRRCALIEEKRRIE
                     EFLFDEGYFAASVDIEPEFAGSTATVKVDVRLQNWFQLGTITVACPAGYVRPKNSSTC
                     VDPTTNTAFALAMPEADIKAMFVHERCLVGSYGCFLGPARFTRAQYQADLQKLKEKFQ
                     HDYNYPGVRITSSELAASIDRKHGRVNPVLTIEQRRQVFVDFTGQDSDRVSDSQLRDQ
                     LTFYDAGAADDVEAAESARALTTYLQTRGFFDARVTWTRERVDIEPRPGTRDTGTHFD
                     KITFVIDAKDTRRVQSVQFVGNHELKSDKLAELVATKEARLANTFLGTTASPTSVELI
                     ADQERIKEAYRRAGYPDARVGTSASPVGSSLDNAALTAALLGTDRDNNLFVRFTIDEG
                     PPTLLSRVIVQLDGGGQPDPALCAESLGALAKLLDSPQIALQTDKDKCAVTVSDLKFR
                     ADDVAAARDQLRDALFKIGRARARVEYAAVPIGPSRVEARYTIKHVDKLKLGTVVIRG
                     NFRTTNTVIYDALDFDEGDLLTTDRLAEGARRLRNMGLFETVNLDMPDLDCDQDRQCS
                     SEVINAVVRVEERYPHTAEIALEGGFSSQNGFFGTIRPTAYNILGRGMRFDVTLTLGQ
                     RLTEVDARYRIPPYLMRPFDWHLDFTTDFTALYRVQDTDRFGVLETRGFGVALSRAFW
                     VRPRTDKHSARAFTIGPYYDFHVRSHNVDATRPIGANMDENQVAISTRTGAIGVHFEF
                     ENRVDRNGALSPLAPEKGAHAELSASYATPYLGGQDKFLKFSASASKFETFAKYINVR
                     ADARYDQGIPFGGVQLLPDVERFFAGGDNTVRGYADDRMATEIIQVGVPPFDNVTQIR
                     VLPASRNIRVLASLDAQARIWKILAGALFTDAGLLTNQWSSATTDDIRPSVGMGLRAL
                     TPFGIGALEYAVPLRPQLGDDPRGRIHFYFAARAQF"
     gene            complement(7523..>9514)
                     /locus_tag="VFV99_10265"
     CDS             complement(7523..>9514)
                     /locus_tag="VFV99_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX5059735.1"
                     /translation="DRDYALSARVTPSTLELACRNYNVQGGRQPCPARLVTPAGIVEI
                     SGGATAGAMQISALGQSLDLRKLAPLFENQLEDISGTLELNAKVGGTFKKPTYEASLD
                     VKDLVSLRLPGGDSVLQILGPRTVAGEKLPGAQIKIANGTVGFGSFTINVKDERKDEQ
                     GELHVSGSIALAGLKPASWGLLIEGKIAGKMLSAIAPSSLAQGSGLATIDAALGGKGA
                     LPLVNATLTFDPAEGTRAQPLALFPRGVRREIAMLGGSVEITTKEVGAHRTYTLDFRD
                     NPITATIDNEGRLTDVRGELVLADGKLDHADLALDAEKIPFRKAQVLDLNLSARNVSL
                     RLPSADSEWQARGNIAIVNGSYRRNFVLTEVIRPAPETAAPAKPFWDEYPSIGNADLD
                     LELEVRKFAVENNIAKIELAGPRLYITGSPRDPRISGAIRVQSGEFKLPGVRARFLRT
                     VGSIDFAENTKATNPSLDITSDAPDFVDLSGQAHTITLTISGSLEQPQWDLRTSTGYD
                     KSQTLALLLLGRNPEQLRRSLGDQAIGTDPTHTETSTNPTGGVGDQLVKDLAGDWVSG
                     LFGSSLTKFTGLDVLRFEVGFGSVGIRAEKKMLENLRAIFEYEQTVRGSTVNLHGEAR
                     TPLPDLTLQGGYLNKNYDDPSDVDISDGNLKLVYRLFIP"
BASE COUNT         1552 a         3060 c         3178 g         1724 t
ORIGIN      
        1 caacgcgcga tgacgatcgc tcgtcatcgc gcggccgcgg ttctgcgggc gagtcctgaa
       61 cgctacggct cgcagttcgt attcggtacc gagcaggtat cggtgccggg gccaccgtcg
      121 acggtgccca tgccgccgcc ctgggtcagc acgtcgttgc cggcgccacc gaacaggttg
      181 tcgtcacccg gaccaccgtc gaggttgtca tcgccgggac cgccggtgag cgtgtcgttg
      241 ccggtgttgc ccttcagaat gtcgttgcca gcgccgccga ccacgacgtc atcgccgccc
      301 tcgccgtcga tggtgtcatt gccgtcgcca ccgatcagcg tatcgggacc gacgccgcca
      361 ttgatcgtat cgttaccccc accgccgtcg gcgaaatcct ggccctcgtc ggcatcgatg
      421 gtgtcatcgc cttcgccgcc tgtgatcgcg tcggcaccac cgccgccact gatcgtatcg
      481 ttgcccgcac cgccgtcgat cgagtccgga ccgagtccgc catcgatgtg atcggcgccc
      541 gctccgccat acagattgtc ggcgccactg ctgccgtcga tcgaatcgat gccagcgccg
      601 ccatcgacgg tgtcatcacc gccacccgcg aacagcagat ccggaccatc gccgccgtag
      661 atcgtatcgg cgccgacgtc gccgaaaatc acgtccgcgc cgtcattgcc gtagatcgtg
      721 tcctcgccgc tatcgccatc gacaacgtcg tgccccgcgc ctgcgatcac gacgtcgtcg
      781 ccgccaccgg caaacaccac gtcatcaccc tcgagcgcga cgatgcactc ggcttcgttc
      841 gtgccgacga tcacgtcgtc gcctgccgtc ccgaagatca ccggtagcaa gccacactcg
      901 ggcggcacga caggttccgt cggtgcgagc gtcatcactc gcgcgggctc gtcgctgatc
      961 tcgccggcgg cacgcatcgc acgtacatgc tccttgccga gtgcgatcag cgcatcgtga
     1021 tcgagctgca cgtcgtctgt cgcgctgctg tcaagcgcgc tggtatcggg cgcggccgga
     1081 tcggcgctgc gcggctcgtc catgcaggca ccgagcgtca gtgcggccgt cgctgcagcg
     1141 gcaagcggcc agaggtcaaa cttcgccatc actccacgaa tcaatcccgt ctcgaacatg
     1201 gcttctcctt gtggaccgcc cgatcgcatt cgcgaccggg gcgggcgacg ccacctttgg
     1261 cgatcgcgcg gcgcgacggc aatgtgtgaa gccgatgcac gcgacaaagc gtccgacgct
     1321 gtcaggcccg cgccgacgct gtccgctcgc ttccgacgac gtcggttcgc gctggcgagg
     1381 cacaggatcg cgcgccgatc gcgagcgcag tgacgtgtgc gtgcggaatc acgagcgcgc
     1441 ctgcttgtta gcgtcgcgtc agggttcgta cacgatcgga ccgggcatac cgtcgagaca
     1501 gccgttgaga tacgagcgcg cttccttgtt cggaagcgca acacgtgcgc cgcacacggc
     1561 gcggtcagtg cagcgaccga gcaggcgatt gtgggcgaag tcgatgtcga gctctagcgc
     1621 ttcaatggca tcggcgccga ggattccgtc gacttccggg cgatcaggac gaatctccgc
     1681 gcgtaatgac tgcagcgtcg ggttcgtgtc ggggacgatg agcagcggaa tcccagcgag
     1741 cggtgcaagc tcgatgaccg cgggcacgcc gcagaacgtc tcgccgagct gacacggaca
     1801 gtcgtcgccg ggcaggcagt cttgcagcgc gagcaggtgc gatgcccaca tctggcggca
     1861 cggcgcgcgc ggattgctgc ccgagttgct taccagcgcg atgcttgcca gcgtcgtcac
     1921 gtttccaacg acagggccgg acggcaaaaa gacggtggcc tcgggtagcg cgtcgaacgc
     1981 cggcagactg ctgtcgagct cgcgcaggtg ttggtacgcc gagcgactca gcaaggacgt
     2041 gccgaccccg gtcgacaaca cgaacagcgc atcgacgccg cgttgcgcct gcgtggccgg
     2101 cacgccattg gggaagcggt tgatcgggcg cggaagcatg caggagtcga tcgcgatccg
     2161 caggcccggg aacccgatct cggttcctcc gatgacgagc gttccaccac cggagaaggg
     2221 cgagctcatc accgaatcgc aggaccgagc acggcggagg tcgctgcctg cgacgtcggg
     2281 caagatgaag atctggtcgc tgccgagcgc caggcgcaat gcatcgccgg tgaacgcatt
     2341 gaggccgagc actgcgttga acggacgcgg ggccgtttcg gtaccgacgg tgcagtcgcg
     2401 gactgtttcg tcgcacggat ggagtgtggt gacctgaagg tcatcgacct tcgcgcgcgg
     2461 cagcgtgagg tcgtcgcctg ctacgggtgc accgaggatc gtcacatccg tcgtttccat
     2521 tgacgctggg acgactggcc cgcgatcgat caccgtcatc ggcgacatca catcgagcac
     2581 cgcctgacgt tgatcgaggt cgccggcttc ctgcatgccg atgacgactg cgccactcgc
     2641 gttgtcgacg aagatcggga actgatcgcc cgacgagtcg ttggtgtcga agctgctcgt
     2701 caggtagtcg cagcctgcaa gtgcgagcat gccgagacat gcagcgacgc ggttcaccac
     2761 ggcagctcgc gaaccgttgc cttgcggcca tcgatctcga ccgtgccgcc gacatcccac
     2821 accgtgcggt gcagatagcc cagcgcgatc gtcgtgccat cgccggcggc gatcgccgat
     2881 gtgacctcgc cggccttgtc cttgttcgcg tgcttgatga cggtgccgcg cgccacgggc
     2941 gtatcgcctt cgatcacgaa gccgcgcagt acgcgcgccg aattgccctg cgcgtggacg
     3001 cggaacaccg gctcctggcc gacgtaacag cccttgccgt agtcgaggaa ctgcgcgagc
     3061 ggcgtctcga acggaaagtg atcctcgtcg acatcgacgt cgtaacgcat gaagccgccg
     3121 cgaatgcgca tgcgctcggc ggcatcatcg gtctccgcca cgtcgccggc accttccgcg
     3181 atcggcgcgc gccacaccga gaccggctcc tcccacgtct ggtgggcggg cccggtgatc
     3241 ggttcaaacc cgacatcatc catcaccgcg tagcgctcga gcagggcgcg cgtcttctcc
     3301 gcaagcgccg gctcgcacgc gagcaagaat tcctggccga gcttcgccag gttgaacacg
     3361 gtgagcacgc ggcccttcgg actcaggatc gcgccccagc cattcgcgcc atcggcgagc
     3421 gactgcacgt tgatcgtcgt caggccctgc aggaagcgct cgcgatcgcc gccggtgatg
     3481 cgcagacggc cccagttgct ccgatcgatc ttcatgacga tcccttagcg cgcccggccg
     3541 ccttgagccg ctccgcgcgg cgaaactctg catccgcatg gcgccggtgg tcgtcctcgg
     3601 tcaccagctc gagcggcggg atcggccgcg gccggcccat cgcgtcgatc gcgacgaacg
     3661 tcaggtacgc cttcgtgacg tagcggcgct gcccggtcgt catgtcctcg gtctcgaccc
     3721 gacagccgac ctccatcgat gtccgcgcgg tgtagttcac catcgcttgc aagacgacga
     3781 tctcgccgac ccgtacgggc gacaagaaat cgagacggtc gatcgacgcg gtaagcaccg
     3841 cgcccgacgc atgccgcatg gccgcgacgc cggcgcagac gtcgatccat tgcatcatga
     3901 tgccgccgaa catgacgcca gcgacaccgt tggtgtgcat cggcatcacg atctgcgtca
     3961 tcacgcagcg tgtctgctcg ggcgtcacgc ccccagttta cttcttcttc gcaggcgcag
     4021 caggcttctt ggccgccttg gcagtcgcga tgcgcgcctt cgtacggcgc ttcagcttgg
     4081 cctggcgctt cttgcgccgc atcttccagg ttcgacgatt atctccgcgt cccatagggc
     4141 gacgcttata cctaaaattg tgcgcgtgcg gcgaagtaga agtgaattct tccgcgcggg
     4201 tcgtcgccga gctggggcct gagggggacg gcatattcga gggcgccgat gccaaacggg
     4261 gtcagggcgc gcaagcccat gccaaccgag ggccgaatgt cgtcggtggt ggcgctgctc
     4321 cactggttcg ttagcaggcc ggcatcggtg aatagcgcac cggccaggat cttccagatc
     4381 cgggcctggg catccaggct ggcgagcacg cggatgttgc ggctcgccgg caggacgcga
     4441 atctgggtga cgttatcgaa cggcggcacg ccaacctgga tgatctcggt cgccattcgg
     4501 tcgtccgcgt agccgcgcac ggtgttgtcg ccgcctgcga agaaccgctc gacgtccggc
     4561 agcagctgga cgccgccgaa cggaatgccc tgatcgtagc gggcgtcggc gcgcacgttg
     4621 atgtacttcg cgaatgtctc gaacttcgag gccgaggccg agaacttgag gaacttgtct
     4681 tgtccgccga ggtatggcgt cgcgtacgag gccgacagct cggcgtgcgc acccttttct
     4741 ggcgcgagcg gtgacagcgc gccgttccga tcgacgcgat tctcgaattc gaagtgcacg
     4801 ccaatcgcac cggtacgcgt cgagatcgcg acctggttct cgtccatgtt tgcgccgatc
     4861 gggcgtgtcg cgtcgacgtt gtggctgcga acgtgaaagt cgtagtaagg gccgattgta
     4921 aacgcgcgcg ccgaatgctt gtccgtgcgc ggccgaaccc aaaatgcgcg cgatagtgcg
     4981 acgccgaagc cgcgcgtctc gaggactccg aatcggtccg tgtcctggac gcgatacagt
     5041 gcggtgaagt cggtcgtgaa gtcgaggtgc cagtcgaacg gccgcatcag gtacggcggg
     5101 atgcgatagc gcgcgtcgac ttccgtgagc cgctgaccga gcgtaagcgt gacgtcgaag
     5161 cgcatgccgc ggcccaggat gttgtaggcg gttgggcgaa tcgtgccgaa gaacccattc
     5221 tgtgaggaga aaccaccttc gagggcaatc tctgcggtat gggggtagcg ctcttcgacg
     5281 cggacgaccg cgttgatcac ctcgctcgag cattgccggt cctgatcgca gtcgaggtcc
     5341 ggcatgtcta ggttgacggt ttcgaacaag cccatgttgc gaaggcggcg agctccctcg
     5401 gcaagccgat ccgttgtcag caggtcaccc tcatcgaagt cgagagcgtc gtagatgacc
     5461 gtgttcgtcg tgcggaagtt gccgcgaatc accacggtgc cgagcttgag cttgtcgacg
     5521 tgtttgatcg tgtagcgtgc ttccacacgc gaggggccga tcggtacggc ggcgtactcg
     5581 acgcgggctc gtgcgcgacc gatcttgaac agcgcgtcgc gcaattgatc gcgcgccgcc
     5641 gcgacgtcgt cggcacggaa cttcaggtcg ctgacggtga ccgcgcactt gtccttgtcg
     5701 gtctgcagtg cgatctgcgg cgagtcgagc agtttcgcga gcgcgccgag cgattccgcg
     5761 cagagcgcgg gatctggctg gccgccgcca tcgagttgca cgatcacgcg gctgagcagc
     5821 gtcggcgggc cctcgtcgat cgtgaaccgg acgaacaggt tgttgtcgcg gtcggtgccg
     5881 agcagcgccg cggtcagcgc agcgttgtcg agactggagc cgaccggtga tgcgctcgta
     5941 ccgacgcggg catcggggta gccggcgcgg cgatacgcct ccttgatccg ttcctggtcc
     6001 gcgatcaact cgacggaggt cggtgacgcg gtcgtgccga ggaacgtatt cgccagccgc
     6061 gcttccttgg tcgcgacgag ctcggccagc ttgtcacttt ttagctcgtg attgccgacg
     6121 aactgtacgc tctggacgcg acgcgtgtct ttcgcgtcga tgacgaacgt gatcttgtcg
     6181 aagtgagtgc cggtgtcgcg cgtgccgggc cgcggctcga tatcgacgcg ttcgcgcgtc
     6241 cacgtgacgc gcgcatcgaa gaagccgcga gtctggagat acgtggtcag cgcacgcgcg
     6301 gactcggccg cttcgacgtc gtcggcggca ccggcgtcgt agaacgtgag ctggtcgcgg
     6361 agctgagagt cgctgacccg gtcgctatcc tggccggtga agtccacgaa cacctggcgg
     6421 cgctgttcga tcgtcagcac cgggttcacg cgaccgtgct tgcgatcgat cgacgcggcg
     6481 agctcgctcg aggtgatgcg cacgcccgga tagttgtagt cgtgttggaa cttctccttc
     6541 agcttctgga gatctgcctg gtactgcgcg cgcgtgaacc gcgcggggcc gagaaagcag
     6601 ccgtacgatc ccacgaggca acgttcgtga acgaacatcg ccttgatgtc ggcctcgggc
     6661 attgctagcg cgaaggccgt gttcgtcgtg ggatccacac acgtgctcga atttttcggc
     6721 cgcacgtagc cagcggggca cgcgaccgtg atcgtgccga gctggaacca attctgcagc
     6781 ctcacatcga ccttcacggt cgcggtcgaa ccagcgaatt ctggctcgat gtcgacgctt
     6841 gccgcgaagt agccctcgtc gaacaagaac tcctcgatcc gccgcttctc ttcgatgagc
     6901 gcgcaacggc ggcggacatc ggttggtggc ggatcccacg ggaggtacga ccccgagcgg
     6961 aagctcatac gccgaacgat gtcgtcaccg agttgtttgt cgaagagcga gccactgaag
     7021 gagaaagttc gtcgccaatc gaaccgaacc ttgaccttga cctttttcac gagcggcagc
     7081 ggcgcgacat acagcacgag ctgcgtaccg ttcggaatgt ccttcgtgac atggccgatt
     7141 aactgatagc cgaatcgggc gagcacgccg gcgacttgcg gcagcgtcgc ccgcgagagc
     7201 gacgtacgga gctgctgcat cgtcggatcg agcagcgcca tcacggtctt cttgcggtcg
     7261 ataagctgac cttggatgtc gaattcgctc catggaatcg gcggcgcgag ctcgcccggc
     7321 ggcggtggct cctgcagcac ggcacccggc gtcagatcga gctggaggca cgctgcttcg
     7381 tcgaactttg gctgcgtggt cagcgtgtct tcttcagtcg cgggcggtgg ttgcgtcggc
     7441 gattgtgcgg gtgcgggcgc acgaggggcc tgtgcgagtg cgggcgcgct cgccaccacc
     7501 agcatcaata ccagcgcacg cttcacggga tgaatagtcg atacacgagc tttaggttgc
     7561 cgtcggagat gtcgacgtcg gaggggtcgt cgtagttctt gttcaagtag ccgccctgca
     7621 gcgtgaggtc gggaagcggt gtgcgcgctt cgccgtgcaa gttgacggtg ctgccgcgta
     7681 cggtctgctc gtattcgaag atcgcacgga ggttctcgag catcttcttt tcggcgcgaa
     7741 tgccgacgga cccgaagccg acctcgaagc gcaggacgtc gaggccggtg aattttgtca
     7801 gcgagctgcc aaacagtccg gacacccagt cgccggcgag gtccttgacg agctgatcgc
     7861 cgacgccacc ggtcggattc gtcgatgtct cggtgtgcgt cgggtcggtg ccaatcgctt
     7921 gatcgccgag cgagcggcgc agctgctccg ggttgcggcc gagcagcagg agcgccagcg
     7981 tctgcgactt gtcgtagccg gtcgacgtgc gcagatccca ctgcggctgt tcgagcgagc
     8041 cagagatagt gagcgtgatc gtatgcgcct gaccggatag atcgacgaag tctggcgcgt
     8101 cgctcgtgat atcgagcgac gggttcgtcg ccttcgtgtt ctcggcgaag tcgatcgagc
     8161 cgaccgtgcg gaggaagcgc gcgcggacgc cgggcagctt gaactcaccg ctctggacgc
     8221 gaatcgcgcc cgagatgcgc ggatcgcgcg gcgagccggt gatgtagaga cgtggaccgg
     8281 cgagctcgat cttggcgatg ttgttctcga cggcaaactt gcgaacttcg agctcgagat
     8341 cgagatccgc gttgccgatc gacgggtact cgtcccagaa cggctttgcc ggcgcggcgg
     8401 tttcgggcgc cggtctgatc acctcggtga ggacgaagtt gcggcggtag gagccgttga
     8461 cgatcgcgat attgccgcgc gcctgccatt cgctgtccgc actcggcaga cggagcgaga
     8521 cgttgcgcgc cgacaggttg aggtcgagca cctgtgcctt gcggaacgga atcttctcgg
     8581 catcgagtgc gaggtccgcg tgatccagct tgccgtcggc gagcacgagc tcgccacgga
     8641 cgtccgtgag gcggccttcg ttgtcgatcg tcgccgtgat cgggttgtcg cggaagtcga
     8701 gcgtgtacgt gcggtgcgcg ccgacttcct tcgtcgtgat ctcgacgctg ccgccgagca
     8761 tcgcgatctc gcggcggaca ccacgcggga acagcgcgag cggttgcgcg cgcgtgcctt
     8821 ccgcgggatc gaacgtcagc gtcgcgttga cgagcggcag cgcgcccttg ccgccgagcg
     8881 ccgcgtcgat ggtggcaagg ccactgcctt gcgcgagcga gcttggtgcg atcgcgctca
     8941 gcatcttgcc cgcgatcttg ccctcgatca gaagacccca gctcgctggc ttgagccccg
     9001 cgagcgcgat cgagccggag acgtggagct cgccttgctc gtccttgcgc tcgtccttga
     9061 cgttgatcgt gaagctacca aagccgacgg tgccgttcgc gatcttgatc tgggcgccgg
     9121 gtagtttttc gccggcgacg gtgcgcggac cgagaatctg cagcaccgag tcaccacccg
     9181 gcagccgcag cgagacgagg tctttgacgt cgagcgatgc ctcgtacgtc ggtttcttga
     9241 acgtgccgcc gaccttcgcg ttgagctcga gcgtgcccga gatgtcctcg agctggttct
     9301 cgaacagcgg ggcgagcttt cgcaggtcga gcgactggcc gagcgcgctg atctgcattg
     9361 cgcctgcggt cgcgccgccc gaaatctcga cgatgccggc cggcgtcacg agccgcgccg
     9421 ggcagggctg ccggccgccc tgtacgttgt agttgcggca cgcgagctcg agcgtcgacg
     9481 gcgtcacgcg cgcggagagc gcgtagtcac gatc
//
LOCUS       DASUFX010000257         9663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_456409,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000257 DASUFX010000000
VERSION     DASUFX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456409"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFV99_10270"
     CDS             complement(<1..809)
                     /locus_tag="VFV99_10270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059736.1"
                     /translation="MKLANMDGEKRHTVTVGRYLLHRQIARGGMATIHIARLMGDEGF
                     SRIVAAKRLLPEFAEDTEFVAMFMDEARIASKVHHPNVVPVLDLVTTSDEVMLVQEYV
                     HGVPLHVLLRTAHDANRKIPVRIAVCIASQVLAGLNAAHTTVDEMGMPLNIVHRDVSP
                     QNVMIAIDGSARLLDFGVAKATMAAHITRPDTFKGKLAYSAPEQVRGKPCQQSDVYAL
                     SVVLWELLVGHRMHGSQGEAELVATIMGGDLPTISEALSNEADWGAIDSDE"
     gene            complement(833..2215)
                     /locus_tag="VFV99_10275"
     CDS             complement(833..2215)
                     /locus_tag="VFV99_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5059737.1"
                     /translation="MSADESPLLIGHHSALSGTNQALGTNMKLGIELAFKEKNDAGGI
                     RGRMLQLEFRDDAYDPATAEQAARALMDVQVLDGVAPHCPTTQTPPVAPAISDFALKR
                     GPSAVLAMLGNVGTPTMVRAAPVVVETGTVFFGAFTGAATILRDTKCGECAKYIFNVR
                     ASYAQEARATLEFFKRKQVGGTTVNYENLISFDQNDTFGQSGYDGLVQAYKDMFTNFP
                     GSADPTNPIKRFRYTRNDDTSVPAQAAATQTYLAQLLQQQSGTVSVGIMMTDTYGAAT
                     EYITRLRLWQNASDSEQTMLQKATRLKLYFSNVSFVGPDALSERLLNAGTYAVYGTGQ
                     QLSYADGVVVSQVVPNYQNDVSDVVTSYNQLIADSGRQPGFTSLEGYIAGKIFIAGLE
                     RHKGPFTPESLITDFEGLGDLGLGLGAGAGFKPDNHQYLQSVWGTKINSNGTFSNLYF
                     FANNTFSFFE"
     gene            2625..3209
                     /locus_tag="VFV99_10280"
     CDS             2625..3209
                     /locus_tag="VFV99_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059738.1"
                     /translation="MLRTAVFVVLVLGCSKAKDKEPAAPPPTPTPAAADAVAAEPPQQ
                     DAAVAEGSGSAGSAAGSADGSAAGSGSAVAANDFAFDKLSKEDKVKFMKQKVVPAMKP
                     LFQKFDAKNFAVFNCKTCHGKDPQGSKYKMPTSDLPKLDFDAIKAGKEDPKVVEFMSK
                     TVKPEMAKLLNMPEMNESTPKGFGCLACHTMKKK"
     gene            3176..3853
                     /locus_tag="VFV99_10285"
     CDS             3176..3853
                     /locus_tag="VFV99_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5059739.1"
                     /translation="MPRLPHDEEEVSAFIESLEAFKAERLAPIVGAGRMSLEGGATGD
                     ATAMLQVALANEINVSELAALWMPTTAEVDVKLAFARQAGDEAGHFIVVSDRLRALGF
                     DVDSFRPPTESPLFRYLQSLTTTVERIAAGLFTLESIAYGVNENFMAFCTAMGDGETV
                     RIYREYIQPDERAHQQLGQKLLAKYANTPALEQLARDTVAKVLDIAAATRAKAAEKLG
                     SACFPGC"
     gene            complement(3867..5975)
                     /locus_tag="VFV99_10290"
     CDS             complement(3867..5975)
                     /locus_tag="VFV99_10290"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059740.1"
                     /translation="MSSKPEPKKTKGMCIATRCTSVEQFIQMFHRFVDEESFFVSTLN
                     TRAPGLETSFSVQLQDGTPVLRGLCVVMQAWTDGNNPFKTPGVRLGIKRLTANSMVVF
                     EQLLITRSAAKPPPVPAAVVTPMKPVVTKPLAGTVPKATATPPKGTPLKPVSIAIGVP
                     KAILKSPLTETAPAKSAPSRVEVSTPARAASEPIPEPINPEPTDVIEEKTDVREPKKP
                     VAELEAADIKPIDIKPVDIKPIAAKPSEIAFKPIVDKSTDEKPAKPAEPIDPHVQETK
                     VDPHVQETKVDPFAGERAPGSDLVLPANPLMNLSDESLEGYVDCTLYEETGNFFPVDG
                     SDEAGFVDEVVPPPVLAPKPAISRTLTPIPYALHEPRLPTVTPLPPTPTVAAALDAAD
                     AERGDAARSSQGSQSLVVPPPMPSPPMPRESVMVDPELIARGSTPAVDHGSFDSPTLT
                     REPTGRSNFDADDLPLPAKPRPAKSKRGSWWLLGGALGVLVGAVALVLVTMSSSNGGA
                     APKTPPKPAAKEVAQVTPPVDPNKPQETGDSADTDDDGPPPDEPPPGSGPPMVGSGPC
                     KVVVASTPAGSMVALDDKTVGPSPLTVGTSCEKHKLDISHARYQSTTKFVTLAEGKDQ
                     EVEVTLSRPTHVVTVSSNPSGAQIFIDGRSAGTTPSKLSVLGFTTLKLEFKKTGYQPA
                     SAKLYSKVAQDKVAVKLNKW"
     gene            complement(6082..7128)
                     /locus_tag="VFV99_10295"
     CDS             complement(6082..7128)
                     /locus_tag="VFV99_10295"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059741.1"
                     /translation="MADKMKIGELLLLKERIDPWALSHALKEQLQTRQRIVSMLISRA
                     LLDPDEGAMLLSDQLGYPAALQRHLERRDQSVLELLPPQLGSRWVVLPLAYARTGAVV
                     VVARDPTPILGAALEHAMRASVVLSVTPSLQLERMVRAAYGGAGTEEEPMPQYSPSMS
                     DIGQIRLEDDTPLPKSRGRSVSNMFAVPELPERAPLQVSPLDTTLAEIDRAITFSAVE
                     RLVMAYAQRRWRAALLLKVHGVRALGMRGHGKHIPDPEQITLPLSGPSMLSVARDTRH
                     PTKHTPASDIQDELHSLLGLPRAPVAAPVLAGDDVEAVLVVGDVLDGNANDSLAEVDR
                     LADALGAAFVRFSR"
     gene            7153..7305
                     /locus_tag="VFV99_10300"
                     /pseudo
     CDS             7153..7305
                     /locus_tag="VFV99_10300"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
     gene            7378..8100
                     /locus_tag="VFV99_10305"
                     /pseudo
     CDS             7378..8100
                     /locus_tag="VFV99_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
     gene            8361..>9663
                     /locus_tag="VFV99_10310"
     CDS             8361..>9663
                     /locus_tag="VFV99_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5059742.1"
                     /translation="MSHARVRRLQEVIAKATAAPTHVEVARVIVEEGVAAIGGWIGAL
                     WLTSSDATLEIAHWTGVEPGRRFRRIPLGSDSPLALAVRTATPVWIGSRAEYVAAFPQ
                     SEQRVGEPDNPQDDEIACACLPLVAGDGAIGGLMFAYRAEHDFTDEERTFLISIASQC
                     AHAFERLRLLDSLSRLYAAEAEARRRAEDASRAKDEFLALLGHELRNPLAPIVTALSL
                     MKLRRGADHERERAIIERQVHHLVRLVDDLLDIARITKGAVELVRAPVPLARAIAHAA
                     ESAQPLLEDRGHRLSLDLEQELIVDADIHRLVQVVANLLTNAAKYTPDGGHIALSVRG
                     DGATAKITVRDNGVGISPQLLPHVFDMFVQGERAPDRAEGGLGLGLAVVRNLVERHGG
                     RVAATSEGLGKGSTFTVWWPLAHGVTVASLEESQDVELPMRS"
BASE COUNT         1617 a         3051 c         3245 g         1750 t
ORIGIN      
        1 cactcgtcgc tgtcgatcgc gccccagtca gcttcgttcg acagcgcttc gctgatcgtc
       61 ggcaggtcgc cgcccatgat cgtcgcgacg agctcggcct cgccttgcga gccgtgcatg
      121 cgatggccga cgagtagctc ccacagcacg accgagagcg cgtagacatc gctctgctgg
      181 cacggcttgc cgcgtacctg ctcgggggcc gagtaggcga gcttgccctt gaacgtatcg
      241 ggacgcgtga tgtgcgcggc catcgtggcc ttcgcgacgc cgaagtcgag caggcgcgcc
      301 gagccgtcga tcgcgatcat cacgttctgt ggcgacacgt cgcgatgcac gatgttgagc
      361 ggcatgccca tctcgtcgac ggtcgtgtgc gctgcgttca acccggcgag cacctgcgat
      421 gcgatgcaca ccgcgatgcg caccggaatc ttccggttcg catcgtgcgc ggtgcgcaag
      481 agaacgtgca gtggcacgcc gtgcacgtac tcctggacga gcatgacttc gtcggaggtc
      541 gtgacgaggt cgagcaccgg cacgacgttc gggtgatgaa ccttcgatgc gatgcgcgcc
      601 tcgtccatga acatcgcgac gaactcggtg tcttctgcga actcggggag gagtcgcttc
      661 gcggcaacga tgcggctgaa gccttcgtcg cccatcaagc gagcgatgtg aatcgtcgcc
      721 attccgccgc gcgcgatctg ccggtgcagc agatagcgcc caaccgtcac ggtgtgacgt
      781 ttctcgccgt ccatgttggc tagcttcact tcagctcctc actagcgacg ggctactcga
      841 agaacgagaa cgtgttgttc gcgaagaagt acaagttgct gaacgttccg ttcgagttga
      901 tcttggtgcc ccagaccgac tgcagatatt ggtggttgtc aggcttgaag cccgcgcctg
      961 cgccgaggcc gaggccgaga tcgccgagac cttcgaagtc ggtgatgagc gactcgggcg
     1021 tgaacggccc cttgtgacgc tcgagacccg cgatgaagat cttgccggcg atgtagccct
     1081 cgagcgacgt gaagcccggt tggcggcccg agtccgcgat cagctggttg tatgaagtga
     1141 cgacgtcgct gacgtcgttc tggtagttcg gcacgacctg cgagacgacg acgccgtccg
     1201 cgtacgacag ttgctggccc gtgccgtaca ccgcatacgt gcccgcgttg agcaggcgtt
     1261 ccgagagtgc gtcggggccg acgaacgaca cgttgctgaa gtagagcttc aggcgcgtcg
     1321 ccttctgcag catcgtctgc tcgctgtcgc tcgcgttctg ccacaggcgc aggcgcgtga
     1381 tgtactcggt cgccgcgccg taggtgtcgg tcatcatgat gccgaccgac acggtgccgc
     1441 tctgctgctg gagcaactgc gcgagatagg tttgcgtggc agccgcctgc gcgggcacgc
     1501 tggtgtcgtc gttacgcgtg tagcggaaac gcttgatcgg gttcgtcggg tctgcgctgc
     1561 ccgggaaatt ggtgaacatg tccttgtagg cctggacgag accgtcatag ccggactgac
     1621 cgaacgtgtc gttctggtcg aagctgatca agttctcgta gttgacggtc gtgccgccga
     1681 cctgtttgcg cttgaagaac tcgagcgtcg cgcgcgcttc ttgtgcgtag ctcgcgcgga
     1741 cgttgaagat gtatttcgcg cactcgccac acttcgtgtc gcgcaagatc gtcgcggcgc
     1801 cggtgaatgc tccgaagaac accgtgccgg tctcgacgac cacgggtgca gcgcgcacca
     1861 tcgttggcgt accgacgttg ccgagcatcg cgagcaccgc gctcgggccg cgcttgagcg
     1921 cgaagtccga gatcgctggc gcgaccggcg gcgtttgcgt cgtcgggcaa tgcggcgcga
     1981 cgccgtcgag gacttggacg tccatcaacg cacgcgctgc ttgttcagca gtcgctggat
     2041 cgtacgcgtc gtcgcggaac tcgagctgga gcatgcggcc gcgtatgccg cccgcatcgt
     2101 tcttctcttt gaacgcgagc tcgatgccga gcttcatgtt tgtgccgagc gcctggtttg
     2161 tgcccgacaa cgcgctgtgg tggccgatga gcaacggtga ttcatcagca ctcacgcaga
     2221 caggtttgtc gtcgcggttt tcacagacga gaccgccgcc gcaatcgctg tcgagtgaac
     2281 agggctgcgg cgcaaagtcc gtcgaacatc cgacgacgac aaccagaacc gaccaaggtg
     2341 ccagacgagg aagccccgtg tttgcgagtc tcaaattttt cgttgatcgt tcgcaagggc
     2401 gcttaaagcc gacatcagcg tcgacgagag atgtgaacta acgggacgtt gtgcttctcg
     2461 atcttcgtcg cgtgtaatgc gtccaacgaa caaagatcga cgccttgaat caccgccacg
     2521 tcggactgtg cgctggcgcg cgctttgagg acgaagagat ctgggtgagg tcgatcttcg
     2581 acgcgatttg cacacacagc cgcagtgcgc gaggatgccc ggcaatgctt cgtactgctg
     2641 tttttgtcgt actcgtactt ggttgttcga aagccaaaga caaggaacct gctgcgccgc
     2701 ccccgacgcc gacgcctgct gctgccgacg cagtagcggc cgagccgccg caacaagatg
     2761 ctgcagtagc cgagggcagc ggaagcgcgg gtagtgcagc tggcagcgcc gatggaagcg
     2821 cggcgggcag cggttcggcg gtcgctgcga atgacttcgc cttcgacaag ctgtcgaagg
     2881 aagacaaggt gaagttcatg aagcagaagg tcgtgccggc gatgaagcct ctgtttcaga
     2941 agttcgacgc gaagaacttt gctgtgttca actgcaagac ctgccacggc aaggaccccc
     3001 agggcagcaa gtacaagatg ccgacgtcgg atctgccgaa gctcgacttc gacgcgatca
     3061 aggcgggcaa ggaagacccg aaggtcgtcg agttcatgtc gaagaccgtg aagcccgaga
     3121 tggcgaagct gctcaacatg cccgagatga acgagagcac cccgaagggc tttgggtgcc
     3181 tcgcctgcca cacgatgaag aagaagtgag cgcgttcatc gagagtttgg aggcgttcaa
     3241 ggccgaacgg ctcgcaccga tcgtcggcgc tgggcgcatg tcgctcgagg gcggcgcgac
     3301 gggcgatgcg acggcgatgc tgcaggtcgc gctcgcgaac gagatcaacg tcagcgagct
     3361 tgccgcgctg tggatgccga cgacggccga ggtcgacgtc aagctcgcgt tcgcccggca
     3421 agcgggcgac gaggccggcc acttcatcgt ggtctccgat cgcttgcgcg cgcttggctt
     3481 cgacgtcgat agctttcgcc cgccgacgga gagcccgctg tttcgctatc tgcagagcct
     3541 gaccacgacc gtcgagcgga tcgccgccgg cttgttcacg ctcgaatcga tcgcgtacgg
     3601 cgtcaacgag aacttcatgg cgttctgcac cgcgatgggc gacggcgaaa ccgtgcgcat
     3661 ctatcgcgag tacatccagc ccgacgagcg cgcgcatcag caactcggcc agaagctgct
     3721 cgcgaaatat gcgaacacgc ccgcgctcga gcagctcgcg cgcgacaccg tcgcgaaggt
     3781 cctcgacatc gcggccgcga cccgcgcgaa ggccgccgag aagctcggca gcgcctgctt
     3841 tcccgggtgc tagcgcgccg cggcggctac cacttgttca gcttcacggc gaccttgtcc
     3901 tgcgcgacct tgctgtacag cttcgcgctc gccggctgat agccagtctt cttgaattcg
     3961 agcttgagtg tcgtgaagcc gagcacgctg agcttcgacg gcgtcgtgcc cgcgctgcgc
     4021 ccatcgatga agatctgtgc gcccgacgga ttgctcgaca cggtgacgac gtgagtcggc
     4081 cgcgacagcg tgacctcgac ctcctgatcc ttgccctcgg caagcgtcac gaacttcgtc
     4141 gtcgattgat agcgcgcgtg cgagatgtcg agcttgtgtt tctcgcacga cgtgccgacc
     4201 gtgagcggcg agggcccgac ggtcttgtcg tcgagcgcca ccatcgaccc agcaggcgtc
     4261 gacgcgacca cgaccttgca cggcccagaa cccaccatcg gcggacccga gcccggcggt
     4321 ggttcgtcgg gcggcggccc atcgtcgtcc gtgtcggcgg aatcgccggt ctcttgcggc
     4381 ttgttcggat cgaccggtgg cgtgacctgc gcgacctctt tcgccgcggg cttgggcggc
     4441 gtcttcgggg cggcaccgcc gttcgagctc gacatcgtaa cgaggacaag cgcgacggca
     4501 ccgacgagca caccgagtgc gccgccaagc agccaccacg acccgcgctt tgatttcgca
     4561 gggcgcggct tggcgggcag cggcaggtcg tcggcgtcga agttcgagcg gcctgtcggt
     4621 tcgcgcgtca gcgtcggtga atcgaagctg ccgtggtcga cggccggcgt gctaccgcgc
     4681 gcgatcagct cgggatcgac catcacgctc tcgcgtggca tcggcggcga cggcatcggc
     4741 ggcggcacga ccaacgattg cgaaccttga ctcgaccgag ccgcatcgcc tcgttcggca
     4801 tcggctgcgt cgagcgcggc ggccacggtg ggcgtgggag gcagcggcgt gacggtaggg
     4861 agccgtggct cgtgcagcgc gtacgggatc ggcgtcagcg tgcgcgagat ggccggcttt
     4921 ggcgcgagca ccggcggtgg cacgacttca tcgacgaagc ccgcttcgtc cgaaccgtcg
     4981 accgggaaga agttaccggt ctcctcgtag agcgtgcagt cgacgtagcc ctcgagcgac
     5041 tcgtcggaca ggttcatcag cgggttcgcg ggcagcacca ggtcggagcc cggcgcgcgc
     5101 tcgcccgcga atggatcgac cttggtctcc tgcacgtgcg gatcgacctt cgtctcctgc
     5161 acgtgcggat cgatcggctc cgccggcttg gcgggtttct catccgtcga cttgtcgacg
     5221 atcggcttga acgcgatctc gctcggcttg gcggcgatcg gcttgatgtc gactggcttg
     5281 atgtcgatcg gcttgatgtc ggccgcttcg agctcggcca ccggcttctt cggctcgcga
     5341 acgtcggtct tctcttcgat gacgtcggta ggctcgggat tgatcggctc gggaatcggc
     5401 tccgacgctg cgcgcgcggg cgtgctgacc tcgacgcgcg atggtgcgga cttcgcgggt
     5461 gccgtttcgg tgagcggcga cttcaggatc gccttcggca caccgatcgc gatcgacacc
     5521 ggcttgagcg gcgtgccttt cggcggcgtc gccgttgcct tcggcacggt gccagcgagc
     5581 ggcttggtca cgaccggctt catcggcgtc acgacagcgg ccggtaccgg cggcggcttc
     5641 gcggccgagc gcgtgatcag gagctgctcg aacacgacca tgctgttcgc ggtgagccgc
     5701 ttgatgccga gtcggacgcc gggcgtcttg aacgggttgt taccgtcggt ccacgcctgc
     5761 atcacgacgc acagaccgcg cagcaccgga gtgccgtcct gcagttgcac cgagaacgac
     5821 gtctcgaggc cgggagcgcg ggtgttgagc gtcgacacga agaacgattc ttcgtcgacg
     5881 aaccgatgga acatctggat gaattgctcg acgctggtgc accgcgtggc gatgcacatg
     5941 cccttcgtct tcttcggctc tggcttcgag ctcaccgcgc cccgatgcta attgtaggcg
     6001 ccgggtacgc ctcgtgacca gggaggcgat gtaggcaagc ggccgaccgt gctgcgagcg
     6061 cggacaccga cttttcccgc cctatcgcga gaatcggacg aacgcggcgc ctagcgcatc
     6121 ggcgagccga tcgacctcgg cgagcgaatc gtttgcgttc ccgtcgagca cgtcaccgac
     6181 aacgagtacg gcctcgacgt cgtcgcccgc gagcaccggc gcagcgacgg gagcgcgcgg
     6241 tagcccgagc aggctgtgca gctcgtcctg gatgtcgctg gccggcgtgt gcttcgtcgg
     6301 atggcgggtg tcacgcgcga ccgagagcat cgaggggccc gacagcggca gcgtgatctg
     6361 ctcggggtcg gggatgtgct tgccgtgacc gcgcatgccg agtgccctca cgccgtggac
     6421 cttcagcagc agcgcggcgc gccagcgcct ctgcgcgtag gccatgacga gccgctcgac
     6481 ggccgagaac gtgatcgccc gatcgatctc ggcgagcgtc gtgtcgagcg gcgagacctg
     6541 cagtggtgcg cgctcgggga gctcgggtac ggcgaacata ttcgagaccg aacgaccgcg
     6601 tgatttcggc agcggcgtgt cgtcctcgag ccggatctga ccgatgtccg acatcgacgg
     6661 cgagtactgc ggcatcggct cttcttctgt gcccgcgccg ccatacgctg cgcggaccat
     6721 ccgctcgagc tgaaggctcg gcgtcaccga cagcacgacc gatgcgcgca tcgcatgctc
     6781 gagcgcagcg ccgaggatcg gagtcggatc acgcgcgacg acgacaaccg cgccggtgcg
     6841 cgcatacgcg agcggcaaca cgacccagcg cgacccgagc tgcggcggca acagctcgag
     6901 cacactctga tcgcgacgct cgaggtgacg ttgcagtgcg gccgggtagc caagctgatc
     6961 ggagagcagc attgcgccct cgtccggatc gagtagcgcg cggctgatca acatcgaaac
     7021 gatgcgctgc cgcgtttgta gttgttcttt aagggcgtgc gacaaggccc acggatcgat
     7081 tcgctccttg agcaggagga gctcaccgat cttcatcttg tccgccacct ggtagtgata
     7141 cacccaaccg cgatgaacga cggactctgg tacatcggga tcaacgggca acaacaaggg
     7201 ccgctcggga ctcagcaagt gttcgagctc attcgcagcg gtcaggtcac gcaggccgcc
     7261 tacgtctatg gccaggcgag tccgaagtgg acgccgattt tgcaggtccc cacattcgcc
     7321 ggggcatttc acgcagctac gccgccaccg ccgccgcccc cgccaggtcc cgcgccgatg
     7381 gtggccgacc agatcgactt cgaggtgttc ggcgaagaaa tgcaattcgt cgagatcacc
     7441 ctcgaccccg gtgaagcggc ggtcgctgaa gcgggcgcgt tcttctatat ggaccccggg
     7501 atcaagatgg agacgatttt cggggacggc tcgcagcagg cccagcaggc aggcttcatg
     7561 ggcaagctgc tgcagggcgc gaagcgcgtg ctgaccggcg aatcgctgtt catgacgatc
     7621 ttcggcaatg gcgcccagca gcgtcagaag gtttcgttcg cggcgccgta tccgggaaag
     7681 atcatcccga tcgatctacg cgcgcacggc ggcacgctgc tgtgtcagaa ggacgcgttc
     7741 ttgtgcgccg cgaaaggcat ctcggtcggc gtcgcatgga acaagaaagt cggtgccggt
     7801 ctgttcggcg gcgaaggttt catcctgcag aagctcgaag gtcaggggct cgcattcatt
     7861 cacgcgggcg gcacgatcgt cgcgaaggag ctcgccgcgg gtgaaacgtt gcgcctcgac
     7921 accggctgtc tcgtcgcgtt cgagccgcgc gtcacgtacg acatccagtg ggtcggcgga
     7981 ttcaagagcg cgctgttcgg tggcgagggt ttgttcttcg cgaccctcac cggtccgggc
     8041 cgcgtgtggt tgcagtcgct gccattctcg cgacttgccg gtcgcatgct cgcggccgca
     8101 gtggcgtcgg ggttcgggcg cggacccgac gaaggctcgg cgctcggcgg cttcggcatg
     8161 ggttcgtgag ctgaagctgg cgcagaccac gccgaatctt cgacgcaggt tgtcgtaagt
     8221 gcggcggttg aagaattttc cgccaacgcg gagcaacaaa ctgttgcgtc gagctctact
     8281 ccgttggtgc gcgtaccaat tctgcgatgc gtgtttgcga cacgttccgt ttttgaagcg
     8341 cctgagtaac atcggccggc ttgagtcacg ctcgcgttcg ccgattgcag gaagtcatcg
     8401 cgaaagcgac ggccgcgcct acgcacgtcg aggtcgcgcg cgtgatcgtc gaagaaggtg
     8461 tggctgcgat cggtggttgg atcggcgcac tgtggttgac gtcatctgat gccacgctcg
     8521 agatcgcgca ctggactggt gtcgagcccg gccggcggtt tcgccgcatt ccgcttggca
     8581 gtgactcgcc actcgcactc gcggttcgca cggcgacgcc ggtgtggatc ggcagtcgcg
     8641 ccgaatatgt cgccgcgttt ccgcagtccg agcagcgcgt gggcgagccg gacaatccgc
     8701 aggacgacga gatcgcatgt gcctgtctgc cactcgtcgc cggcgacggc gcgatcggtg
     8761 gcctcatgtt cgcgtaccgc gccgagcacg acttcaccga cgaggaacgt acgttcctca
     8821 tctcgatcgc gagccagtgc gcgcacgcat tcgagcgctt gcgtttgctc gactcgctga
     8881 gccgcctcta cgccgcggaa gccgaagcgc gtcggcgtgc cgaagacgcg agccgcgcca
     8941 aggacgagtt cctcgcgctc ctcggtcacg agctccgcaa tccgctcgcc ccgatcgtga
     9001 ccgcgctcag cctgatgaag ctgcggcgcg gtgccgatca cgaacgcgag cgcgcgatca
     9061 tcgagcgcca ggtccatcac ctcgtgcggc tcgtcgatga tctgctcgac atcgcgcgca
     9121 tcacgaaggg agcggtcgag ctcgtacgcg caccggttcc gctcgcgagg gcgatcgcgc
     9181 acgcagccga gtcggcacag ccactgctcg aggatcgcgg ccatcggctc tcgctcgatc
     9241 tcgaacaaga gctgatcgtc gacgccgaca tccatcgtct cgtgcaggtc gtcgcgaact
     9301 tactgacgaa cgcggcaaaa tacacgcccg acggcggcca catcgcgctc tcggtgcgtg
     9361 gcgacggcgc taccgcgaag atcacggtgc gcgacaacgg cgtcggcatt tcgccgcagc
     9421 tcttgcctca cgtgttcgac atgttcgtgc agggcgagcg ggcgccggat cgcgccgagg
     9481 gtggtctcgg gcttggcctc gcggtcgtca ggaatctcgt cgagcgccat ggcggtcgcg
     9541 tcgcggcgac gagcgaggga ctcggcaagg gttcgacgtt cacagtatgg tggccactcg
     9601 cgcatggcgt cacggtcgcg tcgctcgagg agagccaaga tgtcgagctg ccgatgcggt
     9661 cga
//
LOCUS       DASUFX010000258        15127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1290601, whole genome shotgun sequence.
ACCESSION   DASUFX010000258 DASUFX010000000
VERSION     DASUFX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290601"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFV99_10315"
     CDS             complement(<1..517)
                     /locus_tag="VFV99_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059743.1"
                     /translation="MRIAELIADVPAAQGYLALATERGNREYLLGPRTDTDATPPLLD
                     WRTAPLAEAFFRAAPGEPFELEVGERVTSGTVTARYVIAEHGRALIGDEGVWRPHGEH
                     WREERTHRPAPAAGPPLDRSELPILDPEQQRAVDLPADTSLVVDGEAGVGKTLVALYR
                     IAALARLKAAKT"
     gene            complement(599..2104)
                     /locus_tag="VFV99_10320"
     CDS             complement(599..2104)
                     /locus_tag="VFV99_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5059744.1"
                     /translation="MKRIAYARIAQETNALSPVATTLHDFEGSHYLEGSALLSATTDG
                     PEVPGFFKRAELAGFVAAARARKAEVEPVPILSAWASSGGPLAQDCFEALEERLVEGL
                     KRAGNLDGMYLCLHGAMGARGITDPETRLVRAVRSVIGNAPLVVSHDLHGNVTQARVD
                     AVDALVAYQTNPHRDHARIGKKAGAIVIGTVLGELSPRVAWRSLPMILGGGKTIDFLQ
                     PMRAVFRRMRQAEKSGEALATSTFMVHPWNDSPSLGWSTLVVTDRDERAADRLADELA
                     EMCWERRHEQPPQFPNASEAIATARAARWRRKLGCVTMADASDVVTAGAAGDSTHLLR
                     ALIEEGSGLTSYAAVRDPQAIEKLWHNREGDVVALAIGGTLDPASSPPLPVRGALVSK
                     HERHGFGRTIVLAVEHVRIVITEGPSMVMRPSFYTEVGLDPFKADIVMVKNFFPFLMF
                     FLPYNRKTIFVRTRGTTDFDAAYKLSFDGPMHPRDAVDDWRARDRLRRGIA"
     gene            complement(2101..3432)
                     /locus_tag="VFV99_10325"
     CDS             complement(2101..3432)
                     /locus_tag="VFV99_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5059745.1"
                     /translation="MSATIPANGRTRDEILADLRAKSAGDTKWRDGRVFSLVYHAGEQ
                     HEQLLREAHALYASTNLLNPMAFGSLKQMEREIIEMAAGLFHGPRSCVGTVTSGGTES
                     ILCAVAAYRDRARRKRPWIVWPELVVPTTIHPAFDKAAHYFGVKLVKVPVGPDFRADV
                     RAMAKAITWKTIGLCASAPQYAHGVVDPIEELGALAQKKRLPLHVDACVGGFVLPWLE
                     QLGRPMPLWDFRVAGVTSISADLHKFAYAGKGASTLVWRSIDDMRHQIFVATDFPGGI
                     YASPTMLGTRPGGPIAAAWATLQSFGADGYRELAHKAADAADRLRAGITATPGLALLG
                     KSNSTIVSYAALGADVYALADRLEARGWTVDRQQRPASIHLTVTANHAAIVDDYIRDL
                     RAALAEVRANPALAKSGTAPMYGMAAKMPVRRLVAANVRKVIADLYREGTS"
     gene            complement(3429..4727)
                     /locus_tag="VFV99_10330"
     CDS             complement(3429..4727)
                     /locus_tag="VFV99_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5059746.1"
                     /translation="MARFYSLLVVAMLASQAHAGGFGIPEIGVRRTAMGAVIGRPDEL
                     SALYHNPAGLVLADEWRIYMSAGLSLLSTEFELAPWDRSDEFLGTMPEANGYYAPVKP
                     NRAMGVVPMLATGGRVWNKLYAGAALYVGNATGAAFDEGAVTHYHLIDGYVVAPQLSV
                     TAAYKLRDDLALAATVGVINLRVHGKRYVFPVIDGNDISSLAGAKPELVLDGEAWAPT
                     WSVSAYGKPHPRVTWGAVVVGKVTATLEGPIQITYSDDASQPGDKLMGRQKTEQMLPW
                     TFQGGANVDVTPNLEIGSELRYWLYRQYKRQHTDVVGIFLVRELDTEKNYTDSWELTG
                     GARVHGLAAVPGLDLMLGMQFDKSPAPAKTVTLDQPSFSHWGLHTGARYSVGRYRFGA
                     SYIHYWYEIPTITDSITLPPSNVRGHGANNIMTLSVEASL"
     gene            complement(4787..5581)
                     /locus_tag="VFV99_10335"
     CDS             complement(4787..5581)
                     /locus_tag="VFV99_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5059747.1"
                     /translation="MARRESLAFDTKFAASVSGKPEAVGHLFLMSAGRWLHATGETLQ
                     APVAFLLGDDEIERLHKTSRTFRTDGAHVDVVQLRIAKPNLRAPVGIANGPIALPPPA
                     WDAIRQLVAHAPRGDWQDVARLLDALASANVVTASMPKTVIAEEPERFRRLWEALEPL
                     YSTYGATTSLKQLASSLGMSMRQVGRDAKELSAAFGIGGGYRDALLMVRLRAAVLLLS
                     APEATVADVAKVVGYGSPIALARAFRDAKLPSPSVVQAAIRGEPVK"
     gene            5709..7976
                     /locus_tag="VFV99_10340"
     CDS             5709..7976
                     /locus_tag="VFV99_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5059748.1"
                     /translation="MAVVLAVSAQVATAQDANQVLRGTVVNEDNGAPIEGATVIGQLD
                     TAATDQDGSFALIVGADERYLVVSAPGFAMRSIGVDNASRIELTASHEVIEIEGTAPR
                     PRRPKPPPQEPPGRPAAQSYALSAADLRTLPGTANDALRAAQVLPGVARLPYSFGGIV
                     MRGTSPRDNIVYLDGIEVPLAFHFGGISSFYPSNMLDGITVSNGGIDAGYGRAQGGMV
                     EMHSREPRGGHWRTGGSIGLLDTSVIAEGPYKGGAVMMGVRRSYLDIVMAPFVDDDVP
                     LPSYLDAQFKGSFGNPRITGRITPTAFLAFDYLDATTPKEDREDESTIAAFFIRFALP
                     YERQWGPLSLRFTPWLGTNQLRFTSRVNSVTEKFSRPIYPGGARLDLAREYTWGDIRT
                     GIDLEGGHLSHFQAGLGHKGDILQQINGDTTIDWIDAAIWGETRFLLGRLDLKPGLRL
                     EHYGLTRESVVDPRLALSLPLSKTLTLKQSLGRFHQPPTPGDVDPNGGNPNVKSSYYD
                     STSLGIEIADGPWSGSVTGFYSDGELQGVRTHDVVDFENLGSLGPTFAQLLEKQLGLA
                     FYRENIGRARNTGIELLIKRYTKRWFGLVSYTLSKATRVDDPTRWLVPQTTGSATGTL
                     NDPNILHGPRPFDLDQRHNLNVAGSVVVGNWRLGGRLQYVSGAPYSPMSQGGQDATIE
                     MPYAGRLPDFFQLDVRADRVWRRCWGTVDLYFDIQNLTNRRNVEGRQLDDFGAETDVR
                     GLPILPFIGVEFIPD"
     gene            7990..9411
                     /locus_tag="VFV99_10345"
     CDS             7990..9411
                     /locus_tag="VFV99_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5059749.1"
                     /translation="MRRLPLLVLAACSNTTTNHQMDAGPDAKPYYDARTDAVGSPDAY
                     AARPCDAPVSFAEGLVPTRVLHVEAGAVGGDGSAEKPFGSIAAAAAIATPGTFIQLGL
                     GNHATNQFIPNLHGTATAPIWIGGVQGTHPKINGGAEGLHLTAPQYVVIQNLELHGQS
                     ANGIDIDDSVGNANDAHHVTLSSVDVYDVLETGSGTEAGIKASGVNDLAIHDSVVLRC
                     GIGIELVGVHDSVVARNVVSGSLLHGIRARGGSTDVDIRQNKLRDNGTTGVVLGGSTP
                     FSQFRPPLSMTAPNAEARRVRSFDNVVTGASSSGFSFISCVDCLVAHNLVFGSPLRLV
                     GIYEGNSSQNGYTFELTKNGRVINNSFVWTSPISFGHVDVGVGSSAATFTFSHDLWLA
                     LSTPSQSTPYLGTTFNPVPDTGAVIGIGTGYSADPTVYCGGPEASTATPLPEVDGTIE
                     GWCRSASVPTIGPLMLSEGACDL"
     gene            9408..9971
                     /locus_tag="VFV99_10350"
     CDS             9408..9971
                     /locus_tag="VFV99_10350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5059750.1"
                     /translation="MSDDPIEWRLGTRYPGHDYKVFHTSFVDGTHPRCGTKRFSVIEA
                     VDWVNVIALTPDEHVVLIRQFRVGTAKVCLEIPGGMVDPGEDAQTAAARELVEETGYT
                     ARRWERLGAVAPNPAIQNNTLHTFLALDSERTHEQKLEGSEVVTVETVPLRDVTELLK
                     RGAIDHALVVTAFGHLAFRLGELHRPT"
     gene            9968..11224
                     /locus_tag="VFV99_10355"
     CDS             9968..11224
                     /locus_tag="VFV99_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HEX5059751.1"
                     /translation="MSVTVVAHAARSGIAALNVVTAALDTDPRTRDVPIIFAKSRTEL
                     AAAIAAADTAVVAHSFYSPDAPRAAEDLAWLRANAPAALHVAGGVHATAESAATLRAG
                     FDVVIAGEGEHAFVELVAAAASARDPRGGPGTVWLAGDEIERGPAAPRHPLDDFPAAN
                     LRHGRWNPLEITRGCVYACSFCQTPFMFKARFRHRSVDNVREHVRTLVGHGLQYVRYV
                     SPTSLSYGSDDTSVNLPAVEALLAAVRAELPAGGRQYFGTFPSEVRPEHVSVEALELI
                     KRYADNRSLVIGGQSGSERLLAATHRGHDVAAIERAVALSVEAGMRPDVDFLFGLPGE
                     TDDDRAATIALAERITSRGARVHSHAFMPLPGTPLAGAEPTPITRDVADAVGRLESRG
                     AAYGQWRQQQAVASELVQLRRTRPAR"
     gene            11410..12276
                     /locus_tag="VFV99_10360"
     CDS             11410..12276
                     /locus_tag="VFV99_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059752.1"
                     /translation="MRGMVALAVAAVGCGRIDFDPVPGGASCNWSGGPRLFNEHVRDD
                     VSTPLLDSDPHLVRGDPLTLLFASDTMTDSAEIYTAHRTGLDAPFDPPTLITELSTTA
                     GENGLVLDRSGHGYFAREIGGDQELVEVQRSAGLLQIVRELDELNGPLNQNDPYPSPD
                     GLWLWFATDTVSAKQDIMLAHRPDRSAAWGNVEPFEYNTSEGEASPTLTDDQRVVLWA
                     GRSPDGTNGDIYYATRPTIEDPWSAPERLALETDIHENEPSIRGDGCELFFVRSLSSM
                     SLDWNIYSVDIE"
     gene            12323..12556
                     /locus_tag="VFV99_10365"
     CDS             12323..12556
                     /locus_tag="VFV99_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059753.1"
                     /translation="MPPKTSCPVSLTQFLEKAEPLKVSINGQEMLAEVKQFSTGSFGW
                     YMNGKTVVTVDGKAISVQIGMNLTVVGSKDAER"
     gene            12567..13391
                     /locus_tag="VFV99_10370"
     CDS             12567..13391
                     /locus_tag="VFV99_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5059754.1"
                     /translation="MLVYLSVDMEGVAGVVHVDQTRRTGHDYDRARVWMTAETNAAIE
                     GAFSAGATSVLVNDSHGDMRNLELGQLDRRAELISGSLKPLSMVQGISRDAACALFVG
                     YHAGAASQAGILDHTYYGRVVARLRVGGRDWNETALNAAVCGELGVPVALVTGDQTVC
                     AQAREHLGDVVTVQVKDAITRYSARSLLPAVACERIRDGAAEAVRRATSLAPFRPPAP
                     FDLEIDFVNSACADAAELVPATRRTSGLACAYRAPDAATLIRVIQAWTILAGTTIV"
     gene            13404..14813
                     /gene="asnS"
                     /locus_tag="VFV99_10375"
     CDS             13404..14813
                     /gene="asnS"
                     /locus_tag="VFV99_10375"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX5059755.1"
                     /translation="MSSIRIAKVLAGEVAVGSKVTIEGWIRTRRDSKAGLSFLAIHDG
                     SCFEPLQVVVPQELPNYQSDVMRITTGCAVRATGEVVASQGKGQAVEVRADSVEVVGW
                     VDDPDTYPMQPKRHTMEYLREVAHLRPRTNVIGAVTRVRHTLAQAVHRFFHEHGFFWI
                     HTPIITASDAEGAGQMFKVSTLDYANLPRTPDGKIDFDKDFFGREARLTVSGQLNVET
                     YCMAMSRVYTFGPTFRAENSNTRRHLAEFWMIEPEIAFADLSDDAQLAEDFLKYIFRA
                     LLTERKDDMAFFEKFVDKEAITRLSSMIESKFARLDYTDAIKELDQAQKRGQKFEFEV
                     KWGIDLQSEHEKYLTDTVIKGPVAVVNYPKDIKAFYMRQNADGKTVAAMDVLAPGIGE
                     IIGGSQREERLEVLDARIAEMGLHAKDYWWYRDLRRYGTVPHAGFGLGFERAIIYATG
                     IENIRDVIPFPRAPRQAEF"
     gene            14813..>15127
                     /locus_tag="VFV99_10380"
     CDS             14813..>15127
                     /locus_tag="VFV99_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059756.1"
                     /translation="MDVHAVERAYVAAATGAAQPARAPRTPQALAAAGAVASHFLAVG
                     TPRSIGLVGGDDAAHSLVAHRTWFSPRDIRCTSASLAAAIEGRVVSLAEALTADIVCI
                     HVP"
BASE COUNT         2537 a         5233 c         4885 g         2472 t
ORIGIN      
        1 tggtcttggc tgcctttagc cgagcgagcg cagcgatccg atacagcgcg accaacgtct
       61 tgccgacgcc cgcttcaccg tcgacgacga gcgacgtatc ggcgggcaag tcgaccgcac
      121 gctgctgctc cggatcgagg atcggcagct cgctgcgatc gagcggcggc ccggcagccg
      181 gcgcgggacg gtgcgtacgt tcctcgcgcc agtgctcgcc gtgcggccgc cacacgcctt
      241 cgtcgccgat cagggcgcga ccgtgctcgg cgatgacata ccgcgcggtc accgtaccgc
      301 ttgtcacgcg ctcgccgacc tcgagctcga acggctcacc gggagccgcg cggaagaacg
      361 cctcggcgag cggtgcggtg cgccagtcga gcagcggcgg tgtcgcatcg gtatcggtgc
      421 gcgggccgag caaatactcg cggttaccgc gttcagtcgc gagtgcgagg tacccctgcg
      481 cagcgggcac gtccgcgatc agctcggcga tgcgcatgct cggtgggttt tacgaattct
      541 gcgcgcgacc gcaagctggt aagtgccgcg cggtttgcgt tacgatcttc gggtcaggtc
      601 aggcgatgcc gcggcgcagc cgatcgcgcg cacgccagtc atcgaccgcg tcgcgcggat
      661 gcatcggccc gtcgaacgag agcttgtagg cggcgtcgaa gtcggtcgtg ccgcgcgtgc
      721 gcacgaagat cgtcttgcgg ttgtacggca agaagaacat caggaacggg aagaagttct
      781 tcaccatcac gatgtcggcc ttgaacggat cgagaccgac ctcggtgtag aacgacggcc
      841 gcatcaccat cgacggacct tcggtgatca cgatgcgcac gtgctcgacc gcgagcacga
      901 tcgtgcggcc gaacccgtgt cgctcgtgtt tgctgacgag cgcaccgcgc accggcagcg
      961 gcggactgct cgcgggatcg agcgtgccac cgatcgcgag cgcgaccaca tcgccttcgc
     1021 ggttgtgcca cagcttctcg atcgcctgcg gatcgcgcac cgccgcgtac gacgtgaggc
     1081 cgctaccctc ttcgatcagc gcgcgcaaga ggtgggtcga atcgccggcc gcgccagcgg
     1141 tgacgacatc ggatgcatcc gccatcgtca cgcagccgag cttgcgccgc cagcgcgctg
     1201 cacgcgcggt cgcgatcgct tcgctcgcat tcggaaattg aggcggctgc tcgtggcgac
     1261 gctcccagca catctcggcc agctcgtcgg cgagccggtc ggctgcgcgc tcgtcacgat
     1321 cggtgacgac gagcgtcgac cagccgagcg aggggctgtc gttccacgga tgcaccatga
     1381 acgtcgacgt cgcgagcgcc tcgccgctct tctccgcctg gcgcatgcgg cgaaacaccg
     1441 cgcgcatggg ctgcaggaaa tcgatcgtct tgccgccgcc gaggatcatc ggtagcgacc
     1501 gccacgccac gcgcggcgac agctcgccga gcacggtgcc gatcacgatg gcgccggcct
     1561 tcttaccaat gcgcgcgtga tcgcgatgcg gattcgtctg atacgcgacg agcgcgtcga
     1621 cggcatcgac ccgcgcctgc gtcacgttgc cgtgcaagtc gtgcgacacg acgagcgggg
     1681 cgttaccgat caccgaccgc acggcgcgaa cgagccgggt ctccggatcc gtgatccctc
     1741 gcgcgcccat cgcgccgtgc aggcacagat acatgccgtc gagatttcct gcgcgcttga
     1801 ggccctcgac gagccgctcc tcgagcgcct cgaagcagtc ctgtgcgagt ggtcctcccg
     1861 acgacgccca cgccgatagg atcggcaccg gctcgacctc ggctttgcgc gcgcgggccg
     1921 cggcgacgaa gcctgccagc tcggcacgct tgaagaaccc tggcacctcg ggaccgtcgg
     1981 tcgtcgcgct gagcagcgca ctgccttcga gatagtgact accctcgaag tcgtgcaacg
     2041 tcgtcgcgac cggcgacagc gcgttcgtct cctgggcgat gcgtgcgtac gcgatgcgct
     2101 tcacgacgtc ccctctcgat agaggtccgc gatcaccttg cggacgttcg ccgcgacgag
     2161 ccggcgcacc ggcatcttag ccgccatccc gtacatcggt gcggtgccgc tcttcgcgag
     2221 cgccggattc gcgcggacct cggcgagcgc tgcgcgcagg tcgcggatgt agtcgtcgac
     2281 gatcgccgcg tggttcgcgg tgacggtgag atggatcgat gccggccgct gctggcgatc
     2341 gacggtccag ccacgggcct ccagtcgatc ggcaagcgcg tagacatccg caccgagcgc
     2401 ggcgtagctg acgatcgtcg agttgctctt gccgagcagt gcgagcccgg gcgtcgcggt
     2461 gatcccggcg cgcagccggt ccgcagcatc ggctgccttg tgcgcgagct cgcgatagcc
     2521 gtcggcgccg aacgattgca gcgttgccca cgccgccgcg atcggtccgc ccggccgggt
     2581 gccgagcatc gtcggcgatg cgtagatgcc gcccggaaaa tccgtcgcga cgaagatctg
     2641 gtggcgcatg tcatcgatcg agcgccacac gagcgtcgac gcgcccttgc ccgcgtacgc
     2701 gaacttgtgg aggtcggcgc tgatcgacgt cacgccggca acgcggaaat cccacaacgg
     2761 catcggccgg ccgagctgct cgagccacgg caacacgaag ccaccgacgc acgcatcgac
     2821 gtgaagcggt aaccgcttct tctgcgcaag cgcgccgagc tcttcgatcg gatcgacgac
     2881 gccatgcgcg tattgcggcg ccgacgcaca cagaccgatc gtcttccacg tgatcgcctt
     2941 cgccatcgca cgcacgtcgg cgcggaagtc gggtccgacc ggcaccttca cgagcttcac
     3001 gccgaagtag tgtgccgcct tgtcgaatgc cggatggatc gtcgtcggca cgacgagctc
     3061 gggccacacg atccacggcc gcttgcgccg cgcacgatcg cgatacgcgg cgaccgcgca
     3121 caggatcgac tcggtaccac ccgacgtcac ggtcccgacg cagctgcgcg gaccatgaaa
     3181 tagcccggcg gccatctcga tgatctcgcg ctccatctgc ttcagcgatc cgaacgccat
     3241 cgggttgagt agattcgtcg acgcgtacag cgcgtgcgcc tcgcgcagca attgctcgtg
     3301 ctgctcgccg gcgtgataga cgagcgagaa cacgcggcca tcccgccact tcgtatcgcc
     3361 cgcgctcttc gcgcgcagat cggccagaat ctcgtcgcgc gtacggccgt tcgccggaat
     3421 cgttgcgctc ataagctcgc ctccacagac aacgtcatga tgttgttcgc gccatggccg
     3481 cgcacgttcg acggcggtag cgtgatcgaa tcggtgatcg tcggaatctc gtaccagtag
     3541 tggatgtagc tcgcgccgaa ccggtagcgg ccgaccgagt agcgcgcgcc ggtgtgcagc
     3601 ccccaatgcg agaacgacgg ctggtcgagc gtcacggtct tcgccggcgc tggtgacttg
     3661 tcgaactgca tgcccagcat gagatccaac ccaggcactg ccgcgaggcc atgcacgcgc
     3721 gcaccgcccg tcagctccca cgagtcggtg tagttcttct cggtatcgag ctcgcgcacg
     3781 aggaagatac cgacgacgtc ggtgtgctgg cgcttgtact ggcgatacag ccagtagcgc
     3841 agctcgctgc cgatctcgag gttcggtgtg acgtcgacgt tcgcgccgcc ctggaacgtc
     3901 cacggcagca tctgctcggt cttctgcctg cccatcaact tgtcgccggg ctgcgacgcg
     3961 tcgtcgctgt acgtgatctg gatcggtcct tcgagcgtcg cggtgacctt gccgacgacg
     4021 acggcacccc acgtcacgcg cggatgcggc ttgccgtacg ccgacacgct ccacgtcggc
     4081 gcccacgcct cgccgtcgag cacgagctcg ggcttggcgc cggcgagcga gctgatgtcg
     4141 ttgccatcga tcacgggaaa cacgtagcgc ttgccatgca cccgcaggtt gatcacgcct
     4201 acggtcgcgg cgagcgcgag gtcatcgcgc agcttgtacg cggccgtgac cgaaagttgt
     4261 ggcgcgacga cgtagccgtc gatcaagtgg tagtgcgtga ccgcgccttc gtcgaatgcg
     4321 gcgccggtcg cgttgccgac gtacagcgcg gcgcctgcgt agagtttgtt ccagacgcgg
     4381 ccgcccgtcg cgagcatcgg taccacgccc atcgcgcggt ttggcttgac cggcgcgtag
     4441 tagccattcg cctccggcat cgtgccgaga aactcgtcgc tgcggtccca cggcgcgagc
     4501 tcgaactcgg tcgatagcag cgacagtccc gcggacatat agatgcgcca ctcgtcggcg
     4561 agcacgaggc ccgccgggtt gtgatagagc gcgctcagct cgtcgggccg accgatcacc
     4621 gcgcccatcg cggtccgccg cacaccgatt tcgggaatgc cgaagccgcc cgcgtgcgcc
     4681 tggctggcga gcatcgcgac gacgagcaag ctgtagaacc gggccacgat ccgcaagtac
     4741 caagcagctg gccagcgatc caccacgaag agcggcgtgg ccgattctat ttgaccggtt
     4801 cgccgcgtat cgccgcctgg accacgctcg gcgacggcaa cttggcatcg cgaaaggcgc
     4861 gtgccagcgc gatcgggctg ccatagccga cgaccttcgc gacatcggcg accgtcgctt
     4921 cgggcgccga caacagcagc actgccgcgc gcaggcgcac catgagcagc gcatcgcgat
     4981 agccgccgcc gataccgaac gcggccgaca gctctttcgc atcgcggccg acctggcgca
     5041 tcgacatccc gagactcgac gcgagctgct tgagcgaggt cgtcgcaccg tacgtcgagt
     5101 acagcggctc gagcgcttcc cacagccggc ggaaccgctc cggttcctcg gcgatcaccg
     5161 tcttgggcat cgaggcggtg acgacattcg cgctcgcgag ggcatcgagc aggcgtgcca
     5221 catcttgcca gtcgccgcgc ggagcgtggg cgacgagctg ccggatcgcg tcccacgccg
     5281 gtggaggcag cgcgatcggc ccattcgcga tgcccaccgg cgcgcgcaaa ttcggcttcg
     5341 ctatgcgcag ctgcacgacg tcgacgtgcg ctccatcggt gcgaaacgtg cgcgaggtct
     5401 tgtgaaggcg ctcgatttcg tcgtcgccga gcaggaacgc gaccggtgcc tgcagcgtct
     5461 cgccggtcgc atgcagccag cgccccgccg acatcaggaa caggtggccg acggcttcgg
     5521 gcttacccga gaccgaggcc gcaaatttag tgtcgaatgc cagcgactcc cgtctcgcca
     5581 cgaagccgcg caccgcacgt tcgaacaaaa gcgtcgaacg gaggcccgcg ctgccgatcc
     5641 tgacctcgct agtcgcgacg agcacacgcg gatcgtaata gattcgtgga tgaatgcgac
     5701 ccctggtggt ggccgttgtg ctggctgtct cggctcaggt cgccacggcc caggacgcaa
     5761 accaggtcct gcgcggcacg gtggtcaacg aggacaatgg cgcgccgatc gagggcgcga
     5821 ccgtgatcgg ccagctcgac accgcagcca ctgatcaaga cggcagcttc gcgctcattg
     5881 tcggcgccga cgagcggtac ctcgtcgtca gcgcgcccgg cttcgcgatg cgcagtatcg
     5941 gcgtcgacaa cgcgtcgcgc atcgagctca cggcctcgca cgaagtgatc gagatcgaag
     6001 gcaccgcacc gcggccgcgt agacccaaac cgccgccgca ggaaccgccc gggcggccag
     6061 ctgcgcagtc ctacgcgctg tctgccgccg acctccgcac gctgccgggc accgcaaacg
     6121 atgcgttgcg cgccgcgcag gtactccccg gcgttgcgcg cctgccctac tcgttcggcg
     6181 gcatcgtcat gcgcggcacg tcaccgcgcg acaacatcgt ctacctcgac ggcatcgagg
     6241 taccgctcgc gtttcacttc ggtggcatct cctcgttcta cccgagcaac atgctcgacg
     6301 ggatcacggt cagcaatggc ggcatcgatg ccggatacgg ccgcgcgcaa ggcggtatgg
     6361 tcgagatgca cagccgcgag ccgcgcggcg gccactggcg caccggtggc tcgatcggcc
     6421 tgctcgacac gtcggtgatc gcggagggtc cgtacaaagg tggcgccgtg atgatgggcg
     6481 ttcggcgctc gtacctcgac atcgtgatgg cgccgtttgt cgacgacgac gtcccgttgc
     6541 cgagttatct cgacgcgcag ttcaagggct cgttcggcaa cccgcgcatc acgggccgca
     6601 tcacgccgac ggcatttctc gcgttcgatt atctcgacgc gacgacgccc aaagaggatc
     6661 gcgaagacga gagcacgatc gccgcgttct tcatccggtt cgcgctgccg tacgagcggc
     6721 aatggggccc gctctcgttg cgcttcacgc cgtggctcgg cacgaaccag ctccgcttca
     6781 cgagccgcgt caacagtgtc accgagaagt tctcgcgtcc gatctatccc ggcggcgcgc
     6841 gcctcgatct cgcgcgcgaa tacacgtggg gtgacatccg caccggcatc gatctcgaag
     6901 gcggccacct ctcgcacttc caggccggcc tcggtcacaa gggtgacatc ctgcagcaga
     6961 tcaacggcga cacgacgatc gactggatcg acgctgcgat ctggggcgag acgcgcttct
     7021 tgctcggccg cctcgatctc aagcccggtc tccgactcga gcactacggc ctcacgcgcg
     7081 agagtgtcgt cgatccgcgg ctcgccctca gcctgccgct ctcgaagacg ctcacgctca
     7141 aacagtcact cggccgcttt caccagccgc caacgccggg tgacgtcgat ccgaacggcg
     7201 gcaatccgaa cgtgaagagc tcgtactacg actcgacgtc gctcggcatc gagatcgcgg
     7261 atggcccgtg gtcgggctcc gtcaccggct tctacagcga cggcgagctg caaggtgtgc
     7321 gcacgcacga cgtggtcgac ttcgagaatc tcggctcgct cggcccgacg ttcgcgcagc
     7381 tgctcgagaa gcagctcggg ctcgcgttct atcgcgagaa catcggccgc gcgcgtaaca
     7441 cgggtatcga gctgttgatc aagcgataca cgaagcgctg gttcgggctc gtctcgtaca
     7501 cgctctcgaa agcgacacgc gtcgacgatc cgacgcgctg gctggtgccg caaaccaccg
     7561 gaagcgccac cggtaccctg aacgacccga acatcctgca cggtccacgt ccgttcgatc
     7621 tcgaccagcg ccataacctg aacgtcgcgg gctcggtcgt ggtcggcaac tggcggctcg
     7681 gcggacgcct gcagtacgtg tcgggcgcac cgtactcgcc gatgagccag ggcgggcaag
     7741 acgcgacgat cgagatgccg tacgccggcc ggctccccga cttctttcag ctcgacgtgc
     7801 gcgccgatcg ggtgtggcgc cgctgctggg ggacggtcga cttgtacttc gacatccaga
     7861 acttaacgaa ccggcgcaac gtcgaaggtc ggcaactcga tgacttcggc gccgagaccg
     7921 atgtccgcgg cctgccgatc ctgcctttca ttggcgtcga attcattccg gactgatatg
     7981 gtgtcgccca tgcggcgact cccgctcctc gtgctcgcgg cgtgtagcaa cacgacgacg
     8041 aatcaccaga tggatgccgg gcccgacgcg aagccgtact acgacgcacg caccgacgcg
     8101 gtaggttcgc ccgatgccta tgccgcgcgc ccgtgcgatg cgccggtatc gttcgccgag
     8161 ggcctcgtac cgacgcgcgt gctgcacgtc gaggctggag cagtcggcgg cgatggcagc
     8221 gccgagaagc cgttcggatc gatcgccgcc gccgctgcga tcgcaacacc cggcacgttc
     8281 atccagctcg gccttggcaa ccacgcgacg aaccagttca tcccgaacct gcacggaacc
     8341 gcgaccgctc cgatctggat cggcggcgtc cagggcacgc atccgaagat caacggcggc
     8401 gccgagggac ttcacctgac ggcgccgcaa tatgtcgtga tccagaatct cgagcttcac
     8461 ggtcaatccg cgaacggcat cgacatcgac gacagcgtcg gcaatgcgaa cgacgcacac
     8521 cacgtcacac tctcgagcgt cgatgtctat gacgtcctcg agaccggcag cggcaccgaa
     8581 gcgggcatca aggcatccgg cgtcaacgac ctcgcgatcc acgactccgt ggtgcttcgc
     8641 tgcggtatcg gcatcgagct ggtcggcgtg cacgacagtg tggtcgcgcg caacgtcgtg
     8701 agcggctcgc tgttacacgg catccgcgct cgcggtggct cgaccgatgt cgacattcgc
     8761 cagaacaagc tgcgcgacaa cgggaccacc ggtgtcgtgc tcggcggctc gacgccgttc
     8821 tcgcagttcc ggccaccact ctcgatgaca gcgccgaacg ccgaggcgcg tcgcgtgcgc
     8881 tcgttcgata acgtcgtcac tggcgcgtcg agcagtggct tttcgttcat cagctgcgtc
     8941 gattgtctcg tcgcgcacaa cctcgtgttc ggctccccgt tgcgcctggt cggtatctac
     9001 gagggcaact cgagtcagaa cggctacacg ttcgagctga cgaagaatgg ccgcgtgatc
     9061 aacaacagct tcgtgtggac tagtccgatc agcttcggcc acgtcgacgt cggcgtgggc
     9121 agcagcgccg cgacgttcac gttctcgcac gatctgtggc tggcgctgtc gacgccgtcg
     9181 cagtcgacgc catacctcgg gacaacgttc aacccggttc ccgacaccgg tgcggtgatc
     9241 ggtataggta ccggttactc ggcggatccg accgtgtact gcggcggccc tgaagcgagc
     9301 accgccacgc cgctgcccga ggtcgacggc acgatcgaag gctggtgtcg cagcgcgagc
     9361 gttccgacga tcggtccgct gatgctatcg gaaggcgcgt gtgatctatg agcgacgatc
     9421 cgatcgaatg gcgcctcggc acccgctatc ccggccacga ctacaaggtg tttcacacgt
     9481 cgttcgtcga cggcacgcat ccgcgctgtg gcaccaagcg cttctcggtg atcgaggcgg
     9541 tcgattgggt gaacgtgatc gcgctgacgc cggacgagca cgtcgtgctg atccggcagt
     9601 ttcgcgtcgg caccgcgaag gtctgcctcg agattccggg cggcatggtc gacccgggcg
     9661 aggacgcgca gaccgccgcg gcacgcgagc tcgtcgagga aaccggctac accgcgcgcc
     9721 gctgggaacg gctcggcgcc gttgcgccga atccggcgat ccagaacaac acgctgcaca
     9781 cctttctcgc gctcgattcc gagcgcaccc acgagcaaaa gctcgaaggc agcgaggtcg
     9841 tcaccgtcga gaccgtgccg ctgcgcgacg tcaccgagct cttgaagcgc ggcgcgatcg
     9901 atcacgcgct cgtcgtcacg gcgttcgggc atctcgcgtt ccggctcggc gagctacaca
     9961 ggcccacgtg agcgtcacgg tcgtcgcgca cgcggcgcgc tcgggcatcg ctgccctcaa
    10021 cgtcgtcacc gcggcgctcg acaccgaccc acgcacgcgc gacgtgccga tcatattcgc
    10081 gaagagccgc accgagctcg ccgccgcgat cgcagccgcc gacaccgctg ttgtcgcgca
    10141 ctcgttctac tcgccggatg cgccacgagc cgccgaggat ctcgcgtggc tgcgcgcgaa
    10201 cgcacccgct gcgctgcacg tcgccggcgg cgtgcacgcg accgccgaat cggccgccac
    10261 gctgcgcgcc ggcttcgatg tcgtgatcgc aggcgaaggc gagcacgcat tcgtcgagct
    10321 cgtcgccgcg gccgcgagcg cgcgcgatcc gcgaggtggt cccggcacgg tgtggcttgc
    10381 cggtgacgag atcgagcgcg gccccgcggc accgcgccac ccgctcgacg attttccggc
    10441 tgcgaatctg cgccacggcc gttggaaccc gctcgagatc acgcgtggct gcgtctacgc
    10501 gtgctcgttc tgccagaccc cgttcatgtt caaggcgcgg tttcgccatc gcagcgtcga
    10561 caacgtgcgc gagcacgtgc gcacgctcgt cggtcacggc ctgcagtacg tgcgctacgt
    10621 gtccccgacg tcgctgtcgt acggttccga cgacacgagc gtcaatctgc ccgcggtcga
    10681 ggcattgctc gcggccgtcc gcgccgagct gccggccggc ggcaggcagt acttcggcac
    10741 gttcccgtcg gaggtccgcc ccgagcacgt ctctgtcgaa gcgctcgagc tcatcaagcg
    10801 ctacgccgac aatcgctcgc tcgtgatcgg cggccagtca ggctccgaac gcctgctcgc
    10861 agccacgcat cgcggccacg acgtcgcggc gatcgagcgc gcagtcgcac tcagcgtcga
    10921 ggccggcatg cggcccgacg tcgatttctt gttcgggctg cccggcgaga ccgacgacga
    10981 tcgcgctgcg accatcgcat tggccgagcg catcacgtcg cgcggcgcgc gtgtccactc
    11041 gcacgcgttc atgccattgc ccggcacgcc gctcgccggt gctgaaccaa cgccgatcac
    11101 gcgtgatgtc gcggatgccg tcggccggct cgagtcgcgc ggcgcggcct acgggcagtg
    11161 gcgtcagcag caagccgtcg cgagcgagct cgtgcagctg cgtcgtactc ggccagctcg
    11221 atgagctcgg ccagctcgtg cgcgttcgcg accgcgttgt cgagcggacc gttgcggaac
    11281 acgctcgacg agcacgcgat cggcccacgc cgccgtcccg gtcttgatcg tcgtggtcat
    11341 gccctcgatg aatgcgacga gcatgccgcc ggcacatcga aaaatcgctc agcgcggtaa
    11401 catcgtcgcg tgcggggaat ggtcgcgctg gcggtcgcgg cagtgggatg cggacggatc
    11461 gacttcgatc cggttcccgg tggcgcgagc tgcaactggt caggcggtcc gcgcctgttc
    11521 aacgaacacg tacgcgatga cgtctcgacg ccgctgctcg actccgatcc gcacctcgtg
    11581 cgcggcgatc cgctgacgct actgttcgcg agcgatacga tgacggacag tgccgagatc
    11641 tacaccgcac accggacggg cctcgacgca ccgttcgacc caccgacgct catcaccgag
    11701 ctgtcgacga cagcgggcga gaatgggctc gtgctcgatc gcagcggcca cggttatttc
    11761 gcgcgcgaga tcggcggcga ccaagagctc gtcgaagtgc agcgctcggc cggcctgctg
    11821 cagatcgtgc gcgagctcga cgagctcaat ggccctctga accagaacga tccatacccg
    11881 agtccggacg gactctggct gtggttcgcg accgacacgg tatccgcgaa gcaggacatc
    11941 atgctcgcac accgacccga tcgcagcgcc gcgtggggca atgtcgagcc tttcgagtac
    12001 aacacgagtg agggcgaagc gagtcccaca ctcacggacg accagcgcgt cgtcttgtgg
    12061 gcaggacgct cgcccgacgg gacgaacggc gacatctact atgcgacgcg cccgaccatc
    12121 gaggatccat ggagcgcgcc cgagcgactg gcgttagaga ccgacattca cgaaaacgag
    12181 ccctcgattc gcggcgatgg ctgcgagctg ttcttcgtcc gctcgctatc ctcgatgtcc
    12241 ctcgactgga acatctactc cgtcgacatc gaataacgtg tgagtggcgt cgcggccgag
    12301 tttctgctgt gatgccctcg tcatgccacc caagacttcg tgccccgttt cgctgacgca
    12361 gttcctggaa aaagccgagc ctctgaaggt ctcgatcaac ggccaggaga tgctcgccga
    12421 ggtaaagcag ttctcgaccg gcagcttcgg ctggtacatg aacggcaaga ccgtggtcac
    12481 cgtcgacggt aaggccatct ccgttcagat cggcatgaac ctgaccgtcg tcggcagcaa
    12541 agacgccgag cgctaataag gtctgcgtgc ttgtctacct ctcggtggac atggaaggcg
    12601 tcgctggcgt cgtccatgtc gatcaaacgc gtcgtacggg ccacgattac gatcgcgccc
    12661 gcgtctggat gacggccgag acgaacgccg cgatcgaagg tgcattctcc gccggcgcga
    12721 cgagcgtgct cgtcaacgac agccacggcg acatgcgcaa cctcgagctc ggccagctcg
    12781 accgccgcgc cgagctgatc tccggatcgc tcaagccgct gtcgatggtg caaggcatct
    12841 cgcgtgacgc cgcgtgcgcg ctgttcgttg gctatcacgc cggcgcagcg agccaggccg
    12901 gcatcctcga tcacacgtac tacggtcgcg tcgtcgcccg gctgcgcgtt ggcggccgcg
    12961 actggaacga gaccgcgctg aatgctgcgg tctgcggcga gctcggcgtt ccggtcgcgc
    13021 tcgtcaccgg tgaccagacc gtgtgcgcgc aggcgcgcga acacctcggc gacgttgtca
    13081 cggtccaggt caaggacgcg atcacgcgct actcggcgcg gtcgctgctg cccgccgttg
    13141 cctgcgaacg catccgagac ggcgccgccg aggccgtacg ccgggcaacg tcgctcgcgc
    13201 cgttccggcc gccggcaccg ttcgacctcg agatcgactt cgtgaattcc gcatgtgccg
    13261 acgctgccga gctggtcccc gcgacccgcc ggacgtcggg cctggcctgc gcgtatcgcg
    13321 cacccgatgc cgcgacgctg atccgagtga tccaggcctg gacgatcctc gccggtacga
    13381 caatcgtgta aaacggctgc gtcatgagca gcatccggat cgccaaggtt ctcgcaggcg
    13441 aagttgccgt cggctcgaag gtcacgatcg aaggttggat tcgcacgcgt cgcgactcca
    13501 aggccggcct ttcgttcctc gcaattcacg acggcagctg cttcgagccg ctgcaggtcg
    13561 tcgtgccgca ggagctgccg aattaccaga gcgacgtcat gcgcatcacg accggctgtg
    13621 cggtccgcgc gaccggtgag gtcgtcgcgt cgcagggcaa aggccaagcc gtcgaggtgc
    13681 gcgccgacag cgtcgaagtg gttggctggg tcgacgaccc cgatacgtat ccgatgcagc
    13741 cgaagcgcca cacgatggag tacctgcgcg aggtcgcgca cctccgtccg cgcacgaacg
    13801 tgatcggtgc agtcacgcgc gtacgccaca cgctcgcgca ggccgttcat cgcttctttc
    13861 acgagcacgg cttcttctgg atccacacgc cgatcatcac ggcatcggac gccgagggcg
    13921 ccggccagat gttcaaggtc tcgacgctcg actacgcgaa cctgccgcgc acgcccgacg
    13981 gcaagatcga tttcgacaag gacttcttcg gccgcgaagc acgcctgact gtctccggtc
    14041 agctcaacgt cgagacgtac tgcatggcga tgagccgcgt gtacacgttt gggccgacgt
    14101 tccgtgccga gaacagcaac acgcgccgtc acctcgccga gttctggatg atcgagccgg
    14161 agatcgcgtt cgccgatctc agcgatgacg ctcagctcgc cgaggacttc ctcaagtaca
    14221 tcttccgcgc gctactcacc gagcgcaaag acgacatggc gttcttcgag aagttcgtcg
    14281 acaaggaggc catcacgcgc ctgtcttcga tgatcgaatc gaagttcgcg cgtctcgatt
    14341 acaccgacgc gatcaaggag ctcgaccaag ctcagaagag gggccagaag ttcgagttcg
    14401 aagtgaagtg gggcatcgac ctgcagagcg agcacgagaa gtacctgacc gataccgtga
    14461 tcaaaggccc ggtcgcggtc gtgaactacc cgaaggacat caaagcgttc tatatgcggc
    14521 aaaacgccga cggcaagacc gtcgccgcga tggacgtgct cgcgcccggc atcggcgaga
    14581 tcatcggcgg cagccagcgc gaagagcggc tcgaggtgct cgacgcacgc atcgccgaga
    14641 tgggcctcca cgcgaaggac tactggtggt atcgcgacct gcgtcgctac ggcacggtgc
    14701 cgcacgcggg cttcggcctc ggcttcgagc gcgcgatcat ctatgcgacc ggcatcgaga
    14761 acatccgcga cgtgattccg ttcccgcgcg ccccgcgcca agcggagttt tagtggacgt
    14821 ccacgccgtc gagcgcgcat atgtcgcggc cgccacgggc gcggcgcagc ctgctcgcgc
    14881 gcctaggaca ccgcaagcac tcgcggctgc cggcgccgtc gcctcgcact tcctcgctgt
    14941 cgggacgccg cgcagcatcg gcctcgtcgg cggtgacgat gccgcgcact ccctcgtcgc
    15001 gcatcgcacg tggttctcgc cgcgtgacat ccgctgcaca tccgcgtctc tcgccgccgc
    15061 gatcgaaggc cgtgtcgtgt cgcttgctga agcgctcacg gccgacatcg tgtgcattca
    15121 cgtgccg
//
LOCUS       DASUFX010000259        11365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1707823, whole genome shotgun sequence.
ACCESSION   DASUFX010000259 DASUFX010000000
VERSION     DASUFX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707823"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFV99_10385"
     CDS             complement(<1..332)
                     /locus_tag="VFV99_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HEX5059757.1"
                     /translation="MSILDKILAVKREEVAAARRARAFEELDIVARAAEPTRGLEAAL
                     RRPAGEPIRVLAEIKRASPSAGPIRADADPAEIGADYASAGAAAISVLTDKQFFDGDL
                     RFLAAVRER"
     gene            complement(329..1450)
                     /gene="trpD"
                     /locus_tag="VFV99_10390"
     CDS             complement(329..1450)
                     /gene="trpD"
                     /locus_tag="VFV99_10390"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5059758.1"
                     /translation="MTVAIVRAIGRALDKQDLSRAEMAEVVGQIMDGQATAAQIGGFL
                     IALRAKGEAVEELVGAAAAMRSRATPLTCPHPEHAIDTCGTGGDGAGTINVSTLAAIL
                     IAACGGTVAKHGNRALSSKCGSADVLEALGVTADVEPAIVERCMAMAGIGFAFAPRFH
                     AATRHVGGPRRELGTRTIFNLLGPLTNPANVKHQVIGVYDRRWCEHMAAALGALGLRR
                     AAVVHGAGGIDEIAVRGETHVALWEASEGSVQALSFTPRTFGVDELDPAGLAGGDAAH
                     NAGVLRRVLAGHEVGRGERYEAALRAAAMTAALGLELLEPGELDLARLPDQYLRAAAT
                     VKNGTARLVLHKWRELSQATEGDWAALRALEPSAFVGVE"
     gene            complement(1447..2022)
                     /locus_tag="VFV99_10395"
     CDS             complement(1447..2022)
                     /locus_tag="VFV99_10395"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5059759.1"
                     /translation="MNLLLIDNYDSFTYNLAQYLGELDAKVQVERNDAISVEAVGARK
                     PDAIVISPGPCTPNEAGISMDVIRMYAGQIPILGVCLGHQCIGQVFGGRIVRAPRIMH
                     GKTSKIFHDEKGLFVGVENPFVATRYHSLVIAPESMPDALEITAKTWEDEIMGVRHKQ
                     HFVEGVQFHPESIMTSAGKSLLANFLARARS"
     gene            complement(2019..3542)
                     /gene="trpE"
                     /locus_tag="VFV99_10400"
     CDS             complement(2019..3542)
                     /gene="trpE"
                     /locus_tag="VFV99_10400"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5059760.1"
                     /translation="MYTPSREDFVAAAARGNLIPVYRELLADGDTPVSAYAALGAGEH
                     SFLLESVVGGATWAAYSFVGVSPRALVRWSAGTATVTWFDVDGGGPNSSAEWQTADPT
                     AALDEVLREMRPIEVPGLPRFWGGAVGWLSYDCVRAFEDLPARARPGLEIPALAMVIT
                     DTLLIFDNLRQTMKVVATPYVTRPEKAEAAYDRACARIDAIIAKLREPRRPLPELEPP
                     RFGERGSVVTPPSSFTHDDFVGAVDRVKEYILAGDAFQVVLSQRFSEPAQGARALDVY
                     RALRVINPSPYMFHLQFPEACVTGASPETLVRLSEGRVEVRPIAGTRPRGITAVEDER
                     LALELREDPKECAEHLMLIDLGRNDVGRVAEVGSVHVAEQMVIERYSHVMHMTSHVVG
                     TLAQGKTWLDVLRAAFPAGTLSGAPKIRAMEIIDELEPHQRGIYGGAVGYVSYNGNLD
                     LAIAIRTLVSHGDTIYVQAGAGLVADSVPELEYQETVNKARAVLRAVAMARTAREGL"
     gene            4053..4415
                     /locus_tag="VFV99_10405"
     CDS             4053..4415
                     /locus_tag="VFV99_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059761.1"
                     /translation="MTNPLYLIYLPYAAIAITLTIWLARTLSRHGELFLESVFPERHD
                     LAHAVNQLLVIGFYLVNLGWALLLLRSDQARFIANPTGAIELLATKLGTLLLLLGVAH
                     MANLFVFHRIRQSGKEAA"
     gene            4451..5077
                     /locus_tag="VFV99_10410"
     CDS             4451..5077
                     /locus_tag="VFV99_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059762.1"
                     /translation="MASNDIFARRAGRVAFGVVMMAGVPALVTHALASRRWDEPPAGG
                     WYAWQIIVATWLLAITAGLIVRVVAPHVRMRREADALRDAALWVPSVGVALLMPLTIH
                     MFAFTDDAREFDEWVRLSLAMVSHTHVAFAVLVYVRARALSRGDEPTSIPRIYVLTCA
                     FALFPFVIPVVYVAITGIPIVPLLLWMKRIARLDREHVDVPFAIARVA"
     gene            5077..5760
                     /locus_tag="VFV99_10415"
     CDS             5077..5760
                     /locus_tag="VFV99_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5059763.1"
                     /translation="MPRAKRVAKRPDEKKSEATRRHLLDRALVLFRERGVDATTMRDI
                     AKAAGLSLGAAYYYFPSKEALVFAYYEQNQVVMEELAASATGTLREKLGVLLHGKLRS
                     IHDHRHMLGTIVQRLIDPGDPLSAFSAQTRAVRDRAIAILEKPLRDEGVPDDVARVGA
                     HALWLFMLAMMLVYIKDDSPKQKRTHGLVDDALDMLVPMLPLLATPMGHAMVKRVVGA
                     LDRAGIAVG"
     gene            5796..7055
                     /gene="glyA"
                     /locus_tag="VFV99_10420"
     CDS             5796..7055
                     /gene="glyA"
                     /locus_tag="VFV99_10420"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5059764.1"
                     /translation="MHPALAKTIADVDPEIAELITAEEKRQRDSVRLIASENYVSGAV
                     LAATGSVLTNKYSEGYPGKRYYEGQKYIDRVEQLCIDRAKEVFGVDHANPQPYSGSPA
                     NLAVYFAFLKPGDTVMGMGLPAGGHLTHGWNVSITGSYFRSVQYGVRRDTHRVDMDEV
                     RELARKEKPKLMFCGGTAIPRTIDFPAFGEIAKEVGALLCADIAHIAGLVAAGAHPSP
                     VAVADVVSTTTHKTLRGPRGGMLMCKAEHAKAIDRAVFPGLQGGPHNHTTAGIAVALK
                     EAQNAAFKTYAHQIVTNARALAAALVERGFSLVTGGTDNHLILADLTSKNVSGKIAAK
                     ALDAAGIELNYNSVPFDPRKPFDPSGIRIGTPAVTSRGMKEAEMRQIAVWMDQVVSAP
                     TDAGLHEKIAGEVRELCTKFPAPGILV"
     gene            complement(7136..8476)
                     /locus_tag="VFV99_10425"
     CDS             complement(7136..8476)
                     /locus_tag="VFV99_10425"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX5059765.1"
                     /translation="MPRIADRVVKPPSDELLPKFIASLDPDWIDEALEATGTATIRRR
                     RLPAEQVVWLVIGMCLFRDQSMRELVSMLDLALPGSRGIRVAPSSIVQARARLGDEPL
                     RWIFERTATAWAHDSARTHAWRGLALYGVDGTTLRVPDSRENRAHFGGQDTRWDSTSG
                     YPLARLVTLMSLRSHLLAGARFGAHTTHETVLAEEMWPLIPDHSLAIVDRGFFSARIL
                     IGLERGGIERHWLTRARSDLVSTRIERFATGDELVELTVSRAARKKDPSMPDTWRMRA
                     IRYQRRGFKPQLLLTSMLDPTRFPADEIVALYHERWEIELGYNEVKRVMLAREETTRS
                     KSPQGVAQELWALALAYNLVRFEAERVAAEAGVPPARVSFIAALRFIESALRRWGAES
                     AGRLPERLRHLREDIGHYILPERRQRSYPRAVKIKMSNYDRKRPTKSSSKRRAK"
     gene            complement(8591..9916)
                     /gene="coaBC"
                     /locus_tag="VFV99_10430"
     CDS             complement(8591..9916)
                     /gene="coaBC"
                     /locus_tag="VFV99_10430"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5059766.1"
                     /translation="MLEGPRSDRAPRDPAFALTGAQIVVGVGGGIAAYKAAELVRMLD
                     KAGATVHVAMTPRAQKFIAPMTFQALTRRPVFTDLFSLTEEATIGHIQLADRADLVII
                     APATANVIARLAHGEAADAVTAMVLATKAPVLVAPSMNVNMWNHPITRANVQRLVDVA
                     KYKVVGPGEGFLACRWTGPGRLAEPADIVEAAAMLLSRQDLAGKKLVVSAGPTYEAID
                     PVRFVGNRSSGKMGIALAAAAQRRGADVTLILGPSSVPPPIGVTTNHVETAAQLQWAL
                     GNASKGADAIVMTAAVADYRPAREAKEKLKRGELGAKTSIELVANPDLLAELGKQRKG
                     KTPLLVGFAAETEDVIENARKKLEQKKCDLIVANDVSEPNAGFAVDTNHVQLVDADDV
                     VDVPSAPKSEVAHRILDRIAEMLGEPVAARSSVPASTKRAAKQAKRKKR"
     gene            complement(9950..>11365)
                     /locus_tag="VFV99_10435"
     CDS             complement(9950..>11365)
                     /locus_tag="VFV99_10435"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5059767.1"
                     /translation="RDAYIDGAWHGGPRRFAVRDPFDDTVVAEVADCDDTLIDAAVAA
                     AARAFPDWRRRPSGERGKLLGQLAARMLADEQRLAELCTRENGKPLKESLAEVRYAAS
                     FFSWFAGEAERMYGETIPASNVGQRLMAVREPVGPCALITPWNFPYAMLTRKLGAALA
                     AGCTAVAKPAQQTPLGALAIAQLAEQVGIPAGVVNVVPSSDAARVGAKLLGAPQIKKI
                     SFTGSTAVGISLMRAAADDLKRISLELGGNAPFIVLGDADPEAAVAGALVAKFRNTGQ
                     SCVAANRFIVEQPIASVFTAKFSAAIAKLKVGRGLDAGIDIGPVIDDKAVAKIKRLVD
                     DAIAKGARVLLGEAPSGKTRLIAPIVLVDIKSDMQLWREEIFGPVVAIRTAKNEADAI
                     ALANDTEYGLVAYAYTRDGARQVRVAEALESGMVGINEGLVSTSQAPFGGIKHSGVGR
                     EGSRHGIDDYTNLKYIATRIG"
BASE COUNT         1810 a         3899 c         3745 g         1911 t
ORIGIN      
        1 cgttcgcgca ccgccgcgag aaatcgcagg tcgccgtcga agaattgctt gtcggtgagc
       61 accgaaatcg cggcggcacc ggcgctcgcg tagtcagcgc cgatttcggc cggatccgcg
      121 tcggcacgaa tcggccccgc cgacggcgaa gcgcgcttga tctcggcgag cacgcgaatc
      181 ggctcgcccg ccggtcggcg gagcgccgct tccagtccac gcgtcggttc ggcagcacgc
      241 gcgacgatat ccagctcctc gaacgcgcgt gcacgacgcg cggccgcgac ctcctcgcgc
      301 ttcacggcga ggatcttgtc gagaatgctc actcgacccc cacgaacgcg ctgggctcga
      361 gcgcgcgcag cgccgcccag tcgccttcgg tggcctggct gagctcgcgc catttgtgga
      421 gcacgaggcg cgcggttccg ttcttcacgg tggccgccgc gcgcagatac tgatccggca
      481 aacgcgcgag gtcgagctca cccggttcga gcagctcgag gccgagtgcg gctgtcatcg
      541 cggccgcgcg caaggccgcc tcgtaccgct cgccgcggcc gacctcatgg ccggcgagta
      601 ctctgcgcag cacgccggca ttgtgcgcgg catcgccgcc cgcgaggccc gcagggtcca
      661 gctcgtcgac gccgaacgtg cgcggtgtaa acgacagcgc ctgcacactg ccttcgctcg
      721 cctcccagag cgcgacgtgg gtctcaccgc gcaccgcgat ctcgtcgatt ccacccgcgc
      781 cgtgaacgac ggcagcgcgg cgcaagccga gcgcgccgag cgcggccgcc atgtgctcgc
      841 accagcgccg atcgtagaca ccgatcactt gatgcttcac gttcgccgga ttcgtcagcg
      901 gcccgagcag gttgaagatc gtgcgcgtgc cgagctcgcg acgcgggcca ccgacgtgac
      961 gcgtggccgc gtgaaagcgc ggcgcgaacg cgaagccgat gccggccatc gccatgcagc
     1021 gctcgacgat tgcgggctcg acatcggcgg ttaccccgag cgcctcgagc acgtcggccg
     1081 agccacactt cgacgatagc gcgcgattgc cgtgcttcgc gaccgtgccg ccgcacgctg
     1141 cgatcaggat cgctgcgagc gtcgagacat tgatcgtacc ggcgccatcg ccgccggtac
     1201 cgcacgtgtc gatcgcgtgc tccggatgcg ggcacgtcag tggcgtcgcg cgactgcgca
     1261 ttgcagccgc agcgccgacg agctcctcga ccgcctcgcc tttcgcacgc agtgcgatca
     1321 agaaaccgcc gatctgcgcg gcggtcgcct gtccgtccat gatctggccg acgacctcgg
     1381 ccatctcggc gcgcgacaga tcctgcttat cgagcgcacg accgatcgcg cgaacgatag
     1441 cgaccgtcat gaccgcgctc gcgcgaggaa gttcgcgagc agcgacttac ccgccgacgt
     1501 catgatcgat tcgggatgga actgcacgcc ttcgacgaag tgctgcttgt ggcgcacgcc
     1561 catgatctcg tcctcccacg tcttcgccgt gatctcgagc gcatcgggca tcgactcggg
     1621 cgcgatgacg agcgagtggt agcgggtcgc gacaaacgga ttctcgacgc cgacgaacag
     1681 gcccttctcg tcgtggaaga tcttcgacgt cttgccgtgc atgatgcgcg gcgcgcgcac
     1741 gatgcggccg ccgaacacct ggccgatgca ctggtggccg aggcagacgc cgaggatcgg
     1801 aatctgcccg gcgtacatgc ggatgacgtc catcgagatg ccggcttcgt tcggcgtgca
     1861 cgggcccggc gagatcacga tcgcatccgg cttgcgcgcg ccgacggcct cgacgctgat
     1921 cgcatcgttg cgctcgacct gaaccttcgc gtccagctcg cccaggtact gcgcgaggtt
     1981 gtacgtgaac gagtcgtagt tatcgatcag gaggaggttc acaggccctc ccttgcagtg
     2041 cgcgccatcg ctaccgcgcg cagcaccgcg cgcgccttgt tgaccgtctc ctggtactcg
     2101 agctcgggca ccgaatccgc aacgaggccg gcgccggctt gcacgtagat cgtatcgccg
     2161 tgactgacca gcgtgcgaat cgcgatcgcg agatcgaggt taccgttgta actgacgtag
     2221 ccgacggcac cgccgtaaat gccgcgttga tgcggttcga gctcgtcgat gatctccatg
     2281 gcgcgaatct ttggagcgcc cgacagcgtg cccgccggga acgcagcacg tagcacgtcg
     2341 agccaggtct tgccttgcgc gagcgtgccg acaacgtgcg acgtcatgtg catgacgtgc
     2401 gaatagcgct cgatgaccat ctgctctgcg acatgcaccg agccgacttc ggctacgcgg
     2461 ccgacatcgt tgcggccgag gtcgatcagc atcaagtgct cagcgcattc ctttggatcc
     2521 tcgcgcagct cgagcgcgag ccgctcatct tcgacagcgg tgatgccgcg aggccgggtg
     2581 cccgcaatcg gtcgcacctc gacgcgccct tccgacagcc gcacgagcgt ctcgggcgac
     2641 gcgccggtga cgcacgcctc gggaaactgg aggtggaaca tgtacggcga cggattgatc
     2701 acgcgtagcg cgcgatagac gtcgagcgcg cgagcaccct gcgcgggctc gctgaaccgt
     2761 tgcgacagca cgacctggaa cgcgtcgccc gcgaggatgt actccttcac gcgatcgact
     2821 gcgccgacga agtcgtcgtg ggtaaacgac gacggcggcg tcaccaccga gccgcgctcg
     2881 ccgaaccgcg gtggctcgag ctcgggcagc ggccggcgcg gctcgcgcag ctttgcgatg
     2941 atcgcgtcga tgcgcgcgca cgcgcggtcg tatgccgctt ccgctttctc cggccgcgtc
     3001 acatacggcg tcgcgacgac cttcatcgtc tggcgcaggt tgtcgaaaat cagcagcgtg
     3061 tcggtgatga ccatcgcgag cgcgggaatc tcgagccccg gccgcgcacg cgccggcagg
     3121 tcctcgaacg cacgcacgca gtcgtacgac agccagccga ccgcgccgcc ccagaaccgc
     3181 gggagccccg gcacttcgat cggacgcatc tcgcgcagca cttcgtcgag cgcagcggtt
     3241 ggatccgcgg tctgccattc ggcgctgctg ttcgggccgc caccgtcgac gtcaaaccag
     3301 gtcacggtcg cggtgcccgc agaccagcgc acgagcgcgc gcggcgacac accgacgaac
     3361 gagtacgccg cccacgtcgc gccgccgacg accgactcga gcagaaacga gtgctcgccg
     3421 gcgccgagcg ccgcgtacgc cgacaccggc gtgtcgccgt cggcgagcag ctcgcgatag
     3481 acggggatca ggtttccgcg tgcggctgcc gcgacaaagt cctcgcggct cggggtgtac
     3541 atgggaacgc tggttatatc gcgttcgcag tgagtgcgcg tgccgcgttc tgcggcgatc
     3601 gcgcacggat atcgcggtga atcgcgtgcg gtttctgagt agctcggaag gcgcttccgg
     3661 cggtccggcg ccggttcggc ggcgaggcgg ttcggcgacg aggcggttcg gcggcgaggc
     3721 ggttcggcgg tgcggcggca gttcggcggc ggtcgcggcg gttcggcggc gaggcggttc
     3781 ggcggcgagg cggttcggcg tcggcgcggc gatcgcggcg cttcagcgct tcgggggtgg
     3841 cgcggcgctt cgggggggtc ggcggcggcg catcggcgat gccacggatg gcatcagagc
     3901 caggcgcgga aattccgagc cggcactccc gtcgagatct cgcgttgttt tgaacatgtt
     3961 cagttttggc attgacgatc agcgttcgac tgactattta tttgaacgtg ttcaaaacac
     4021 agttgtgacc cacctgaacc ggagacccgc ccatgacgaa cccgctctac ctcatctacc
     4081 tgccctacgc cgccatcgcg atcacgctca ccatctggct cgcccgcacg ctatctcggc
     4141 acggcgagct gttcctcgag tccgtgttcc ccgagcgtca tgacctcgct cacgccgtga
     4201 accagctgct cgtgatcggc ttctacctgg tcaacctcgg ctgggccctg ctcctgctcc
     4261 gctccgatca ggcccgcttc atcgcgaacc cgaccggcgc gatcgagctg ctcgcgacca
     4321 agctcggcac cctgctcttg ctgctcggcg tcgcgcacat ggccaacctg ttcgtgttcc
     4381 atcgcatccg gcaatccggc aaggaggcgg cgtgaccgcc gacgagtacg tcgtttgccg
     4441 cgcccgacgc atggccagca acgacatctt tgcgcgccgc gcgggccgcg tcgcgttcgg
     4501 cgtcgtaatg atggccggcg tgccggcgct cgtcacgcat gccctcgcgt cgcggcgctg
     4561 ggacgagcca ccggcgggcg gctggtacgc gtggcagatc atcgtcgcga cgtggttgct
     4621 cgcgatcacg gccgggctca tcgtccgcgt ggtcgcgccg cacgtgcgca tgcgccgtga
     4681 agctgacgcg ctccgagacg ccgcgctgtg ggtgccatcc gtcggcgtcg cgctgctcat
     4741 gccgctcacg atccacatgt tcgcgttcac cgacgacgcg cgtgaattcg acgagtgggt
     4801 gcggctcagc ctcgcgatgg tctctcacac gcatgtggcg ttcgccgtcc tcgtgtacgt
     4861 gcgcgctcgc gcgctgtccc gcggcgacga gccgaccagc attccgcgca tctatgtgct
     4921 gacctgcgcg ttcgcattgt tcccgttcgt gattcccgtc gtgtatgtcg cgatcaccgg
     4981 gatccccatc gtgccgctgc tgctgtggat gaaacgcatc gcgcgccttg atcgcgagca
     5041 cgtcgatgta cccttcgcca tcgcacgagt tgcctgatgc cgcgcgccaa gcgagtcgcc
     5101 aaacgtccgg acgagaagaa gagcgaagct acccgtcgcc acttgctcga tcgcgcgctc
     5161 gtgttgtttc gcgagcgcgg cgtcgacgcg acgacgatgc gcgacatcgc gaaggccgcc
     5221 ggactctcgc tcggcgccgc gtactactac ttcccgtcga aggaagcgct cgtgttcgcg
     5281 tactacgagc agaaccaggt cgtgatggag gagctcgcgg cgtcggcgac cggcacgctg
     5341 cgcgagaagc tcggtgttct gctccacggc aagctgcgct cgattcacga tcatcgccac
     5401 atgctcggca cgatcgtgca gcggctgatc gatccgggcg atccgctgtc ggcgttctcg
     5461 gcgcagacgc gcgccgtgcg cgatcgcgcg atcgcgatcc tcgagaagcc gctacgcgac
     5521 gagggagttc cggacgacgt cgcccgcgtc ggcgcccacg cgctgtggct gttcatgctc
     5581 gcgatgatgc tcgtttacat caaggacgac agcccgaagc agaagcgcac gcacggcctc
     5641 gtcgacgacg cgctcgacat gctcgtgccg atgctcccgt tacttgcgac gccgatgggg
     5701 cacgcgatgg ttaagcgcgt cgtcggcgca ctcgatcgcg cgggcatcgc agtgggctag
     5761 tggtacctgg gatctcgatc gggtatgaac gggacatgca ccccgccctc gccaagacga
     5821 tcgccgacgt cgaccccgaa attgcggaac tgatcaccgc cgaagagaag cgccagcgcg
     5881 attcggttcg tctgatcgcg tcagagaatt acgtgtcggg tgcggtgctc gccgcgactg
     5941 gctcggtgct gacgaacaag tacagcgagg gctatccggg taagcgctac tacgaggggc
     6001 agaagtatat cgatcgcgtc gagcagctct gcatcgatcg cgcgaaggaa gtcttcggcg
     6061 tcgaccacgc gaacccgcag ccgtactcgg gctcgcctgc gaacctcgcc gtctatttcg
     6121 cgttcctcaa gccgggcgac accgtgatgg gcatgggcct accggccggc ggccacctca
     6181 cgcacggctg gaatgtgtcg atcaccggca gctacttccg cagcgtgcag tacggcgtcc
     6241 gccgcgacac gcaccgcgtc gacatggacg aggtccgcga gctcgcgcgc aaagagaagc
     6301 cgaagctcat gttctgtggc ggcaccgcga ttccacgcac gatcgatttc ccggcgttcg
     6361 gtgagatcgc gaaggaagtc ggcgcgctgc tctgcgcaga catcgcgcac atcgccggtc
     6421 tcgtcgcggc gggtgcgcat ccgtcacccg tcgccgtcgc cgacgtcgtg tcgacaacga
     6481 cgcacaagac cctgcgcggt ccgcgcggcg gcatgctgat gtgcaaggcg gagcacgcga
     6541 aggcgatcga tcgcgcggtg ttccccgggc tgcagggcgg cccgcacaac cacacgacgg
     6601 cagggatcgc ggtcgcgctc aaggaagcgc agaacgcggc gttcaagacg tacgcgcatc
     6661 agatcgtcac gaacgcgaga gcactcgccg cggcactcgt cgagcgcggc ttctcgctcg
     6721 tcacgggcgg cacggacaac cacttgatcc tcgccgacct cacgagcaag aacgtgtccg
     6781 gcaagatcgc cgcgaaggca ctcgacgccg ctggcatcga gctcaactac aacagcgtgc
     6841 cgttcgatcc gcgcaagccg ttcgacccgt ctggcatccg catcggtacg cccgcggtca
     6901 cgagccgcgg catgaaggaa gcggagatgc ggcagatcgc ggtctggatg gatcaggtcg
     6961 tcagcgcgcc gaccgacgcc ggcctgcacg agaagattgc cggcgaggtg cgcgagctgt
     7021 gcacgaagtt cccggcgccc ggcatcctcg tctgagtgcg aaagcacgtg acgaccgccg
     7081 cacgggcggc ttcgtcttgc tctacgtcgg ctggcagcct tagcgccaat gccggtcatt
     7141 tagcgcgcct cttcgacgaa gatttggtcg gtcgcttccg atcgtagttg ctcatcttga
     7201 tcttcacggc gcgcggatag ctccgttggc gtcgttcggg aaggatgtag tggcctatgt
     7261 cctcgcgtag gtggcgcaat cgctctggca agcgtcccgc tgattccgcg ccccacctgc
     7321 gaagggcgct ttcgatgaag cggagagcgg cgatgaagct cacccgtgcc ggaggcactc
     7381 cagcctcggc tgcgacgcgc tccgcctcga agcggaccaa gttgtatgca agtgcgagcg
     7441 cccagagttc ttgggcgact ccctgtgggc tcttgctgcg cgtggtttcc tcgcgcgcga
     7501 gcatcacgcg tttgacctcg ttgtagccaa gctcgatctc ccagcgctcg tggtacagcg
     7561 ccacgatctc gtcggcagga aaccgcgtcg gatcgagcat tgaggtcagc agaagctgcg
     7621 gcttgaagcc gcgacgctga tagcgaatcg cgcgcattcg ccaggtgtcg ggcatcgacg
     7681 gatccttctt gcgcgccgcc cgcgacacgg tgagctcgac cagctcgtcg cctgtcgcga
     7741 accgctcgat gcgtgtgctg accaagtcgc tgcgtgcccg ggtgagccaa tggcgctcga
     7801 taccgccgcg ctcgaggccg ataaggatgc gcgccgagaa gaagccgcgg tcgacgatcg
     7861 cgagcgagtg atcggggatc agtggccaca tctcctcggc gagtacggtc tcgtgagtcg
     7921 tatgtgcccc aaagcgcgcg ccggcgagca gatgcgaccg cagcgacatc agcgtgacga
     7981 gccgcgcgag cgggtaaccg ctcgtgctgt cccagcgcgt gtcttgccca ccaaaatgtg
     8041 cacgattctc cctcgagtcg ggcacgcgca gcgtcgtgcc atcgacgccg tacagcgcca
     8101 ggccgcgcca ggcatgcgta cgtgcactgt cgtgcgccca tgccgtcgcc gtgcgctcga
     8161 atatccagcg cagcggctca tcgccgagcc gagcacgcgc ctgaacgatc gaactcggtg
     8221 cgactcggat gcctcggctc cccggcagcg ctaggtccag catcgagacg agctctcgca
     8281 tcgactggtc gcgaaacaga cacatcccga tgaccaacca gacgacctgt tcggcgggca
     8341 gccgtcgacg acgaatcgtc gcggtaccag tcgcctcgag tgcctcgtcg atccaatcag
     8401 gatcgagact cgcaatgaac tttggtaaga gctcgtcgct cggcggcttc acgactcgat
     8461 cggcgatacg cggcaccaac gcatcctgat cgtccggatc ggccgaaaca aggcccccgg
     8521 acatctgccc gaattccgga gcaacgtcac acgcttgccg ctaaccgatc ggcattggcc
     8581 ttagcgcgcc ttagcgcttc ttgcgcttcg cctgcttcgc cgcacgcttc gtgctggctg
     8641 gcaccgagct cctcgcggcg accggctcgc cgagcatctc tgcgatgcga tcgaggatgc
     8701 gatgcgcaac ttccgacttc ggcgccgatg gcacgtcgac gacgtcgtcc gcgtcgacga
     8761 gctgcacgtg attcgtgtcg accgcgaatc ccgcgttcgg ttcgctgaca tcgttcgcga
     8821 cgatcaggtc gcacttcttt tgctcgagct tcttgcgggc gttctcgatc acatcttcgg
     8881 tctcggccgc aaagccaacg agcagcggcg tcttcccctt gcgctgcttg ccgagctcgg
     8941 cgagcaagtc tgggtttgca accagctcga tgctcgtctt cgcaccgagc tcgccacgct
     9001 tgagcttctc cttcgcttcg cgcgccgggc gatagtccgc gacggctgcg gtcatcacga
     9061 tcgcgtccgc gcccttcgac gcattcccga gtgcccactg taactgcgcc gcggtctcga
     9121 cgtggttcgt cgtcacgccg atcggcggcg gcaccgagct cggtccgagg atcaacgtga
     9181 catcggcgcc gcgacgctgc gctgcggccg cgagtgcgat gcccatcttg ccggagctgc
     9241 gattgccgac gaagcgcacc ggatcgatcg cttcgtacgt cgggccggcg ctgacgacga
     9301 gcttcttgcc cgccaggtcc tgccgcgaca gcagcatcgc cgcggcctcg acgatatcgg
     9361 ccggctcggc gagcctgccc ggcccggtcc accgacacgc gaggaagcct tcgccagggc
     9421 cgaccacctt gtacttcgcg acgtcgacga ggcgctgcac gttcgcgcgc gtgatcgggt
     9481 ggttccacat gttgacgttc atcgacggcg ccacgagcac cggcgccttc gtcgcgagca
     9541 ccatcgccgt caccgcatcg gccgcttcgc cgtgcgcgag ccgcgcgatc acgtttgcgg
     9601 tcgccggcgc gatgatgacg aggtccgcgc gatccgcgag ctggatgtgt ccgatcgtcg
     9661 cttcctcggt gaggctgaac agatcggtga acaccgggcg acgcgtgagc gcctggaacg
     9721 tcatcggcgc gatgaacttt tgggcacgtg gcgtcatcgc gacgtgcacc gtcgcgccgg
     9781 ccttgtcgag catgcggacg agctcggccg ccttgtacgc cgcaatgccg ccgccaacgc
     9841 caacgacgat ctgcgcgccg gtcagcgcga acgcgggatc acggggtgca cggtcagaac
     9901 gcggtccttc gagcatccca ggcactctac gcgagcgcgc cagccgcagc tagccgatgc
     9961 gcgtcgcgat gtacttgagg ttcgtgtagt cgtcgatacc gtggcgcgag ccttcgcggc
    10021 cgacgcccga atgcttgatg ccgccgaatg gagcctgcga cgtcgacacc aggccttcgt
    10081 tgatgccgac catgccggac tcgagcgcct cggcgacgcg cacctggcgc gcgccatcac
    10141 gcgtgtacgc atacgcgacg aggccgtact cggtgtcgtt cgcgagtgcg atcgcgtcag
    10201 cttcgttctt tgcggtgcgg atcgcgacga ccgggccgaa gatttcctcg cgccagagct
    10261 gcatgtcgct cttgatgtcg acgagcacga tcggcgcgat cagtcgggtc ttgcccgatg
    10321 gcgcctcgcc gagcagcacg cgcgcgccct tcgcgatcgc gtcgtcgacg agacgtttga
    10381 tcttggcgac cgccttgtca tcgatgactg gaccgatgtc gatgccggcg tcgagaccgc
    10441 gaccgacctt gagcttcgcg atcgcggcgc tgaacttggc ggtgaacacc gacgcgatcg
    10501 gctgctcgac gatgaagcga ttcgccgcga cacagctctg gccggtgttg cgaaacttcg
    10561 ccaccagcgc gccagcgacc gccgcttcgg ggtccgcgtc gccgagcacg atgaacggcg
    10621 cgttaccgcc gagctcgagc gaaatgcgct tgagatcgtc ggcggcggcg cgcatcagcg
    10681 agatgccgac tgcggtcgac ccggtgaagc tgatcttctt gatctgcgga gcgccgagga
    10741 gcttggcgcc cacgcgcgcc gcgtcggagg acgggacgac gttgacgacg ccggccggaa
    10801 tgccgacctg ctcggcgagc tgcgcgatcg cgagcgcgcc cagcggcgtt tgctgcgccg
    10861 gcttcgcgac tgccgtgcag ccggcggcaa gtgcggcgcc gagcttgcgt gtaagcatcg
    10921 cgtacggaaa gttccacggc gtgatcagcg cgcacgggcc gacgggctcg cggacggcca
    10981 tcaggcgctg gccgacatta cttgccggaa tcgtctcgcc gtacatgcgc tcggcttcgc
    11041 cggcgaacca cgagaagaag ctcgctgcat agcgcacctc ggcgagcgat tctttgagcg
    11101 gcttgccgtt ctcacgtgtg catagctcgg cgagccgctg ctcgtcggcg agcatgcgcg
    11161 cagcgagctg cccgagcagc ttgccgcgct cgccactcgg gcgtcgccgc cagtcgggaa
    11221 acgcgcgggc cgccgccgca accgcagcgt cgatcagcgt gtcatcgcag tcggcgacct
    11281 cggcgaccac cgtgtcgtcg aacggatcgc gaacggcaaa ccggcggggg ccaccatgcc
    11341 aagcaccatc gatgtaggcg tcgcg
//
LOCUS       DASUFX010000260         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1151727, whole genome shotgun sequence.
ACCESSION   DASUFX010000260 DASUFX010000000
VERSION     DASUFX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151727"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFV99_10440"
     CDS             <1..545
                     /locus_tag="VFV99_10440"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX5059768.1"
                     /translation="GVITQNVDGLDTAAGSRTVVELHGALARVRCLACGEITTRTSLQ
                     DRLLAANPNWPARARMATIAPDGDVDLPDELVAGFAIVACTSCSGVLMPDVVFFGGSV
                     PRATLDAAWALFDRAELLLVIGSSLTVFSGYRFVRRAAERGVPIAILNRGPTRGDEHA
                     QLRIDARCGDALPRLVQALA"
     gene            620..1105
                     /locus_tag="VFV99_10445"
     CDS             620..1105
                     /locus_tag="VFV99_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059769.1"
                     /translation="MGIRKLLVIASLGFAFAAKPARAEVGLGIFVGEPTGVDVKLDLA
                     RRSALDFVLGVYSHWDHYNTDGAYGHMTYLVQPMVGHGDSVLVPLRLGIGFAVFDDYD
                     RFRDDINVAARVPFEVGLRFRRTPLEIYFEIALKMTFIDENDNHPFVDLDGGIGLRFY
                     L"
     gene            complement(1102..2289)
                     /locus_tag="VFV99_10450"
     CDS             complement(1102..2289)
                     /locus_tag="VFV99_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059770.1"
                     /translation="MGARSSCLLVLLSACGFSHGVDPGNNTIIDSGLTVDVPEGFMFM
                     DAPIDARQCYGTFENICLTELPTTDLVLDTNKEVNTDTGCPYVFTQSSGQSLCGLVYT
                     DITINARLRALGPRPLVVIATGTFKISATGLVDVGSYKEISGPSTTEIIGAGAATGAT
                     LCGNPSSGANDSGMGTVGAGGGAGGSFGGKGGNGARGASNSGGNGGNASNASATPSFM
                     RGGCRGTSGGSGNMHQPGAGGAGGGAVYLIAGTSIDNQGHIRAGGMGGYGGDTESGGG
                     GGGAGGMIVLDAPTITNSGILNANGGGAGEGGGADSGPGPGGGSRGEQGSSALTGTGA
                     ADGGNMETNGGNGGPGSWATASGGTTGSTAANGGGGGGGGAGVIRIYPTQTLAGTISP
                     DPS"
     gene            complement(2315..2836)
                     /locus_tag="VFV99_10455"
     CDS             complement(2315..2836)
                     /locus_tag="VFV99_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059771.1"
                     /translation="MGNLDEPAFAAVIAACQKCDAKAFEVASYIDRQVNVMFGERNDD
                     GRWTHDGEKFIDGVFRIKCIRCSDVAFSTTACPRCHREGGLADVVGKASRLAVPKRCP
                     TCKGTELTVVGFAPSIVRTGEGRPPSPTPTAHFGDNGFHVAHVMCDGCDWVAVAEGCP
                     LCGGEGPLRARPG"
     gene            2881..3150
                     /locus_tag="VFV99_10460"
     CDS             2881..3150
                     /locus_tag="VFV99_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059772.1"
                     /translation="MGINDAVAAIEKFLLSYDGGGGKPVAVQVRPSGDDIDEIKIWID
                     LGPKKRGVDPESYERAAAAAIREALPGVATFKLDVRAEADPATGA"
     gene            3235..>3521
                     /locus_tag="VFV99_10465"
     CDS             3235..>3521
                     /locus_tag="VFV99_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5059773.1"
                     /translation="MAVLHRGDEPLLVIAGAGTGKTTTLAHRIAQLVLDGADPRRILL
                     LTFSRRASQEMIRRAQRVTKGADLPWAGTFHAIGSRLLRMHALALGLDPAFT"
BASE COUNT          567 a         1201 c         1110 g          643 t
ORIGIN      
        1 agggcgtgat cacgcagaac gtcgatggcc tggacaccgc tgcgggcagc cgcacggtgg
       61 tcgagctgca cggtgcactc gcgcgcgtgc gctgcctcgc atgcggcgag atcacgacgc
      121 gtacgtcgct acaagatcga ctgctcgccg cgaatccaaa ctggccggca cgcgcgcgga
      181 tggcgacgat cgcgcccgat ggcgacgtcg atttgccaga cgagctcgtc gccggcttcg
      241 cgatcgtcgc ctgcacgagc tgcagcggcg tgctgatgcc tgacgtcgtg ttcttcggcg
      301 gcagcgtgcc ccgcgcgacg ctcgatgccg catgggcctt gttcgatcgc gccgagctgc
      361 tgctcgtgat cggatcgtcg ctcaccgtgt tctccggtta tcgcttcgtg cgccgcgccg
      421 ctgaacgcgg cgtgccgatc gcgatcctga atcgcggtcc gacacgtggc gacgagcacg
      481 cacagttgcg catcgatgca cgctgtggcg atgcgttacc gcgtctcgtg caagcactgg
      541 cgtgagcgcg catacatcgc gtgcttcgat aggtgctcga cgcgcgcgcg cgtgcagcga
      601 ctcggttagg atccgacgaa tgggaatccg caagctccta gtcattgctt cgcttgggtt
      661 tgcgttcgcg gcgaaacccg cgcgcgcaga ggtcggcctc ggcatctttg tcggcgagcc
      721 gaccggtgtc gacgtcaagc tcgatctcgc ccgccgttct gcgctcgact tcgtgctcgg
      781 cgtctacagc cattgggacc actacaacac cgatggcgcg tacggccaca tgacgtacct
      841 cgtgcaaccg atggtcggtc acggcgacag cgtgctcgtc ccgctgcgcc tcggcatcgg
      901 cttcgcggtg ttcgacgact acgatcgctt ccgcgacgac atcaacgtcg ctgctcgcgt
      961 gccgtttgaa gttggcctgc gctttcgccg cacgccactc gagatctatt tcgagatcgc
     1021 gctgaagatg acgttcatcg acgagaacga caaccatccg ttcgtcgatc tcgacggcgg
     1081 tatcggcctg cgcttctacc tttagctcgg atcgggcgag atggtgccgg cgagcgtctg
     1141 cgtcggatag atccggatca cgccggcgcc gccaccacca ccaccgccgc cattcgcggc
     1201 cgtcgaaccg gtcgtgccgc ccgacgccgt ggcccacgag cccgggccgc cgttgccgcc
     1261 attcgtctcc atgttgccgc cgtccgcggc gcccgtgccg gtgagcgcgg agctgccttg
     1321 ttcgccgcgg ctgccgccgc ccggacccgg accactgtcc gcaccaccgc cttcgccggc
     1381 gccgccgcca ttcgcgttca agatgccgct gttcgtgatc gtcggcgcat cgagcacgat
     1441 catgccgccc gcgccgccac caccgccgcc cgactcggtg tctccaccgt agccgcccat
     1501 gccgcccgcg cgaatgtgac cttggttgtc gatcgaggtt cctgcgatca gatacacggc
     1561 accgccgcct gcgccgccgg cgcccggttg atgcatgttg ccggagccac cactcgttcc
     1621 gcgacaaccg ccgcgcatga agctcggcgt agccgacgca ttcgaggcgt tacctccgtt
     1681 gccaccgctg ttgctcgcgc cgcgtgcccc gttgccgccc ttgccgccga agctgccacc
     1741 tgcgccaccg cctgcaccga cggtgcccat gcccgagtca ttcgcgcccg atgacgggtt
     1801 gccacacaac gtcgcgccgg tcgctgcacc tgcgccgatg atctcggtgg tgctcggtcc
     1861 gctgatctcc ttgtagctac cgacgtcgac gaggccggtt gcagagatct tgaacgtgcc
     1921 ggtcgcgatc acgacgagag ggcgcgggcc cagcgcacgc agccgcgcgt tgatcgtgat
     1981 gtccgtgtag acgagaccgc acaacgattg tccggacgat tgcgtgaata cgtagggaca
     2041 accggtgtcc gtgttgactt ccttgttcgt gtcgagcacg agatcggtcg tcggcagctc
     2101 ggtcaagcag atgttctcga acgtcccgta gcactggcgc gcgtcgattg gcgcatccat
     2161 gaacatgaag ccctcgggaa cgtcgaccgt gagcccactg tcgatgatgg tgttgttacc
     2221 cggatcgacg ccatgcgaga acccgcatgc ggacaacagc acgagtaggc aactcgagcg
     2281 cgcgcccatc ccctctaggg taagccattt ctggctagcc aggacgtgct ctgagcggtc
     2341 cttcgccacc gcacagcggg cagccctcgg cgacggctac ccagtcgcat ccgtcacaca
     2401 tgacgtgcgc aacatggaac ccgttgtcgc caaaatgtgc ggtcggtgta ggtgacggcg
     2461 ggcgaccctc gcctgttcgc acgatcgacg gcgcgaagcc gaccaccgtg agctcggtcc
     2521 ctttgcatgt ggggcagcgc ttcggaaccg ccaatcgcga tgcttttccg acgacatcgg
     2581 cgagtccacc ctcgcgatga cagcgcggac aggcggtagt cgaaaaggcg acatcgctac
     2641 accggatgca cttgatgcga aacacgccat cgatgaactt ctcgccgtcg tgtgtccagc
     2701 gaccgtcatc gttgcgctcc ccgaacatga cgttgacctg gcggtcgatg tagctcgcga
     2761 cctcgaatgc cttcgcgtcg cacttctgac acgctgcgat gacggcggcg aacgcgggct
     2821 cgtcgaggtt ccccatccgc cgcgcatcct acttcactgg cgcgcgtgta agctgcattc
     2881 atggggatca acgatgccgt tgcggctatc gagaagtttc tacttagcta cgacggtggc
     2941 ggtggaaagc ccgtcgcggt gcaagtgcgg ccgtcgggcg acgacatcga cgagatcaag
     3001 atctggatcg atctcggacc aaagaagcgc ggcgttgatc cggagtcgta cgagcgcgcg
     3061 gcagccgctg caattcgcga agcgctgccg ggagtcgcga cgttcaagct cgatgtccgc
     3121 gccgaggcag atcccgccac gggcgcgtag catctccgca aagttgcacg agatcctgga
     3181 cactgttcaa atgaccaggt ggatccacac gcggccttga acgagcagca acgggtggcg
     3241 gtgctccatc gcggtgacga gcctctcctc gtcatcgcgg gagcggggac gggcaagaca
     3301 accactctcg cgcatcgcat cgcgcagctc gtgctcgatg gcgccgatcc gcggcgcatt
     3361 ttgctcctga cgttctcgcg acgcgcgtcg caagaaatga tccgtcgcgc gcagcgcgtg
     3421 acgaagggtg cggacttgcc atgggcgggc accttccacg cgatcggatc gcggctgttg
     3481 cgcatgcatg cgctcgcgct cggtctcgat ccagcgttca c
//
LOCUS       DASUFX010000261         7745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1151834, whole genome shotgun sequence.
ACCESSION   DASUFX010000261 DASUFX010000000
VERSION     DASUFX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151834"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="VFV99_10470"
     CDS             complement(<1..1987)
                     /locus_tag="VFV99_10470"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX5059774.1"
                     /translation="MTEKWVCIHGHFYQPPRENPWLEAIEPQPSAHPYRDWNERITAE
                     CYRPNTAARVVDGHGQIIQIVDNYQRMSFNVGPTLMSWLEKCAPDVHTALIDADRESV
                     KRFSGHGSAMAQAFNHMIMPLASKRDQATQVKWGIADFKRRFGRAPEGMWLPECAVDT
                     PSLEALAAEGIVFTVLAPHQAKAWRPPGGEWRTTAVDPGRAYKYKLPSGKTIDLFFYD
                     GATAQAVAFERLLSDGHQIISRMTARGAVEDDKPTLCHIATDGETYGHHHRYGDMALA
                     WALSQVEQGWSGTRLTNYGEFRTKVPATWEVQLVENSSWSCAHGVSRWREDCGCNSGG
                     KPGWNQKWRRPLRDSLDWLREQAYSTLETIGGALFKDPWAARDAYIDVLLDGASARER
                     FLTAHVKESLDADSRVRALSLMEMARHAMLMYTSCGWFFDDLSGIETVQCMQYAARVA
                     ELIQAVRGISVEPEMIERLSVARSNLADEGDGRRVWAQRVRPARIDPEKVCAHVAVHA
                     LVEPSAPDNGAVAVEGFGVEFVDRVERRSGRARMVAATVRVKSALTETATALCFAGLH
                     LGEQHVTGGVRPPPATSEWTAIQKELRDSLETGDVFAAQRAIDRHFPGAHLSLGALLP
                     GSRESVLAAVLGDAITEAGKELDETYDQHAPLIRWLVA"
     gene            complement(1984..3879)
                     /gene="glgB"
                     /locus_tag="VFV99_10475"
     CDS             complement(1984..3879)
                     /gene="glgB"
                     /locus_tag="VFV99_10475"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX5059775.1"
                     /translation="MQPLHSTPPAHSRLSADDLFLFNQGTHYRLYEKLGAHPVEGGTY
                     FAVWAPSARSVSVIGDWNYWGHGSDKLRPRESSGIWEGVIKGVGHGTRYKYAITGPDG
                     VTREKADPFAVRAEHPPATASIVWSPHYKWSDATWMKTRGAKNARTAPMSIYELHLGS
                     WRRNHGEVLGYRELGKQIGHHVSQLGFTHVELMPIMEHPFYGSWGYQVTGYFAPTTRY
                     GAPEDFMCMVDELHQRGIGVIVDWVPAHFPTDQHGLGYFDGTHLFEHADPRRGFHPDW
                     TSYIFNYARHEVRAFLISSALNWLDRYHIDGLRVDGVASMLYRDYSRKPGEWVPNEDG
                     SNHDRDAISFLQQFNHAVYTSYPDVQTIAEESTAWGGVSRPPDHGGLGFGYKWDLGWM
                     HDTLKYLEREPIHRRYHHDEITFRAIYQNSENYVLPLSHDEVVHGKGALLSKLPGDPW
                     QKYATLRLLYGYQWALPGKKLLFMGDEIGVWNEWNHDHELDWAAGVHPAHAGIARWLG
                     DLNGAYKAHRALHVRDCNPVGFQWLVADDRDNSVLAFMRWGEGGDPPIVVVANFTPVP
                     REGYRVGVPRHGYWREILNSDSSAYGGSGMGNRGGMHTDAIGAGGYEQSLIVTVPPLG
                     IVLFVAE"
     gene            4060..4419
                     /locus_tag="VFV99_10480"
     CDS             4060..4419
                     /locus_tag="VFV99_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059776.1"
                     /translation="MRLTAVILLLSACSWIGSPERPVTNYTQKTQSCSMAAPLADTIA
                     ASLLITGGLAIAVADNLVIPDVQYRGAATLKIALPAVVIGAVFIPSAVHGWRAKRRCE
                     HALSQTKTGAALATPAR"
     gene            complement(4440..6608)
                     /locus_tag="VFV99_10485"
     CDS             complement(4440..6608)
                     /locus_tag="VFV99_10485"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5059777.1"
                     /translation="MKATNWMAGFALGVSLVAGCVDNGSDKPDASDVKGGPEGKAEAW
                     GSSDNPAMFNANLEYRMAQLPQVGEARNIPWAGNYWPVYEDSINYKWNGSSSDAPSTK
                     YGRAFGVTGVEDGVSRYHGIDAQASRTACTTDSQCNSSLGEACAKRTGQTNGRCIPTW
                     WGICHAWSPAAILLPEPKHAVTYNNVEFKVQDIKALLTLVHDQTDTKFVSLRCDALDA
                     SNEITFDKYGRPNNTNAECRDTNPGTFHVLMTNYLGKQGEAFVYDRTWDGEVWNQPLR
                     GYRITASTEISALEANKLIGVPAEGGTTSTKTGTAAGGAWTQLGTVAVSPGQNFTVVM
                     SGSGDPDLYVKFGSQPTASSYDCRPYESGPAETCNVTVPAGQTQAYLAVNAYGTTAAN
                     FTLNITAGGSIPTTYVFNTNAAKLYKTHMDVDYISESPASTDGNLGSTINNYTHTDRY
                     DYVLEVDSAGKIVGGEWVGSSKRQHPDFVWLPLRASATSVAGGKITYANVKMIYDQSV
                     ADPNGGTTGPVRNLTETGAVVKSAWKQYGPYNVAGGTTFTVSMTGDNDADLYVRKGAA
                     PTSASYDCRPYRSGSEEQCSIVGPGTVYVAVNGYAAASNFSLKINYTEGNGNGGVVPP
                     PPMFSHLAKTGSVTQGEMKMFQLPMPAGKKVVIRTTSTKDVDLYIQWGAAPTTDAYLN
                     RGYTSSGNETVSYTATSNGTLFIGVHGYEAGSFSLNTADQ"
     gene            7083..>7745
                     /locus_tag="VFV99_10490"
     CDS             7083..>7745
                     /locus_tag="VFV99_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059778.1"
                     /translation="MRRIAIEKIDEADVLTEIAAAETERSLRDFAGERAAQLWTSHAC
                     SDDEEEANEGLAGITKLGDQHALAEIAVNAALPAVRKRAFGDMRDPRALAELAKGDAP
                     QDLRLAAVGRIDDGDVLRAIAIDTTLKEVGLAAVDKLDDTDRLENVAQKAKNKAVRQR
                     ARKIVAEMEEAERAKSKPAVPDEVKRRRAEKAQLVREVEGVADSFDFEKVSAQVKAAE
                     DAW"
BASE COUNT         1289 a         2568 c         2504 g         1384 t
ORIGIN      
        1 gcgcgacgag ccagcggatc agcggcgcgt gctggtcgta ggtctcgtcg agctccttgc
       61 ccgcctccgt gatcgcatcg ccgagcacgg cggcgagcac gctctcgcga ctgccgggaa
      121 ggagcgcgcc gagcgagaga tgtgcgcccg gaaagtggcg atcgatcgcg cgctgcgcgg
      181 cgaacacgtc gccggtctcg agcgaatcgc gcagctcctt ctggatcgcg gtccactccg
      241 aagtcgccgg cggtggccgc acgccgcccg tcacgtgttg ctcgccgagg tgaagccccg
      301 caaagcacaa cgcagtcgcc gtttcggtca gcgcggactt cacgcgcacg gttgcggcga
      361 ccatgcgcgc gcgaccactg cgccgctcga cacgatcgac gaactcgacg ccgaagcctt
      421 cgaccgcgac ggcgccgttg tcaggtgccg acggctcgac gagcgcgtgc accgcgacgt
      481 gcgcgcacac cttctccgga tcgatgcgcg ccggccgcac gcgctgcgcc cacacccgac
      541 ggccatcacc ttcgtcggcg aggttcgacc gcgcgacgga gaggcgctcg atcatctccg
      601 gctcgacgga gatgccgcgc accgcctgga tcagctcggc gacgcgtgct gcatactgca
      661 tgcactgcac ggtctcgatg cccgacagat cgtcgaagaa ccagccgcag ctcgtgtaca
      721 tgagcatcgc gtggcgtgcc atctccatca gcgacagcgc gcgcacgcgg ctgtcggcgt
      781 cgagcgattc cttgacgtgg gcggtgagga accgctcgcg cgcggaagcg ccgtcgagta
      841 acacgtcgat gtatgcgtct cgcgcggccc acggatcctt gaacagagca ccgccgatcg
      901 tctcgagcgt cgagtacgcc tgctcgcgca accaatcgag cgagtcacgt agcgggcgcc
      961 gccacttctg gttccagcct ggcttaccgc cgctgttgca gccgcaatcc tctcgccatc
     1021 gagaaacccc gtgcgcgcag ctccagctcg aattctcgac gagctggact tcccacgtcg
     1081 ccggcacctt cgtacggaac tcgccgtagt tcgtgagccg cgtgccgctc cagccctgct
     1141 cgacttgtga tagcgcccac gcgagcgcca tgtcgccgta gcgatgatga tggccgtacg
     1201 tctcgccgtc ggtcgcgatg tgacacagcg tcggcttgtc gtcctcgacg gcaccgcgcg
     1261 cggtcatgcg cgagatgatc tgatggccgt ccgagagcaa gcgctcgaac gcgaccgctt
     1321 gcgccgtcgc gccgtcgtag aagaacagat cgatcgtctt gccgctcggc agcttgtact
     1381 tgtacgcgcg ccccggatcg accgcagtcg tgcgccactc accacccggc ggccgccacg
     1441 ccttcgcctg atgcggcgcg agcacggtga acacgatgcc ctcggccgcc aatgcctcga
     1501 ggctcggcgt atcgaccgcg cactcgggca accacatgcc ttccggcgcg cggccgaacc
     1561 tccgcttgaa atcggcgatc ccccacttca cctgcgtcgc ctgatcgcgc ttcgacgcga
     1621 gcggcatgat catgtggttg aaggcctgcg ccatcgcaga gccgtgaccg ctgaagcgct
     1681 tcacgctctc gcggtcggca tcgatcaacg cggtgtgcac gtcgggcgcg cacttctcga
     1741 gccacgacat cagcgtcggc ccgacgttga agctcatgcg ctggtagttg tcgacgatct
     1801 ggatgatctg cccgtgaccg tcgacgacgc gcgccgcggt attcgggcga tagcactccg
     1861 ccgtgatgcg ctcgttccag tcgcggtacg ggtgcgcgct cggctgtggc tcgatcgctt
     1921 cgagccacgg gttctcacgg ggtggctggt aaaagtgccc gtggatgcag acccacttct
     1981 cagtcactct gcaacgaata gtacgatgcc gagcggcggc acggtgacga tcagagactg
     2041 ttcgtacccg cctgcgccga tcgcatcggt gtgcatgccg ccgcggttgc ccataccact
     2101 tccgccgtat gcggaagagt cggagttcag aatctctcgc cagtagccgt gcctcggcac
     2161 gccgacgcga tacccctcgc gcggcacggg cgtgaaattc gcgacgacga cgatcggcgg
     2221 atcgcctccc tcgccccagc gcatgaatgc gagcacgctg ttgtcgcgat cgtccgcgac
     2281 gagccactga aagccgaccg ggttgcagtc gcgtacgtgg agcgcgcgat gcgccttgta
     2341 cgcgccattg agatcgccga gccagcgcgc gatcccggcg tgcgcgggat gcacgcccgc
     2401 ggcccagtcg agctcgtggt cgtggttcca ctcgttccac acgccgatct cgtcgcccat
     2461 gaacaagagc ttcttgccgg gcagcgccca ctgatagccg tacagcaggc gcagcgtcgc
     2521 gtacttctgc cacgggtcgc cgggcagctt cgacagcagc gcgcctttgc cgtgcacgac
     2581 ctcgtcgtgc gacagcggca gtacgtagtt ctcgctgttc tgatagatcg cgcggaacgt
     2641 gatctcgtcg tggtggtagc ggcgatggat cggctcgcgc tcgagatact tgagcgtgtc
     2701 gtgcatccag ccgaggtccc acttgtagcc aaagccaagg ccaccgtgat caggcggccg
     2761 ggacacgccg ccccacgccg tcgactcctc ggcgatcgtc tgcacgtccg gatacgacgt
     2821 gtaaaccgcg tgattgaact gctgcaagaa cgagatcgcg tcgcgatcgt gattactgcc
     2881 gtcttcattc ggtacccact cgccgggctt gcgcgagtaa tcccgataca gcatcgacgc
     2941 gacgccgtcc acgcgcagcc catcgatgtg atagcggtcg agccagttga gcgcgctcga
     3001 gatcaaaaac gcgcgcacct cgtggcgcgc ataattgaag atgtagctcg tccagtccgg
     3061 gtgaaagccg cgcctgggat cggcgtgctc gaacaggtgc gtgccgtcga agtagccgag
     3121 gccatgctga tcggtcggaa agtgcgccgg aacccagtcg acgatcacgc caatgccgcg
     3181 ctggtgcagc tcgtcgacca tgcacatgaa atcttcgggc gcgccgtacc gcgtggtcgg
     3241 cgcgaagtag cccgtcacct gatagcccca cgagccgtag aacggatgct ccatgatcgg
     3301 catcagctcg acgtgcgtga agccaagctg cgagacgtga tgaccgatct gcttgccgag
     3361 ctcgcgatat ccgagcacct cgccgtgatt gcgtcgccac gatccgagat gcagctcgta
     3421 gatgctcatg ggcgccgtgc gcgcgttctt cgcgccacgc gtcttcatcc acgtcgcatc
     3481 gctccacttg tagtgcgggc tccacacgat cgacgcggtc gccggcggat gctccgcacg
     3541 caccgcaaac ggatccgctt tctcgcgcgt gacgccgtcg ggccccgtga tcgcgtactt
     3601 gtatcgggtg ccgtgaccga cgcctttgat cacgccttcc cagatgccgc tcgactcgcg
     3661 cggccggagc ttgtcactgc cgtggcccca gtaattccag tcgccgatca cgctcacgct
     3721 gcgcgcgctc ggtgcccaca ccgcgaagta ggtgccgcct tcgacgggat gcgcgccgag
     3781 cttctcgtac aaccggtagt gcgtgccttg gttgaacaga aacagatcat ccgcagacag
     3841 acgcgaatga gcgggcggtg tcgagtgaag cggctgcacg cgagcagatt accggctagg
     3901 ggcgggagca caagaagctc ttactaacga aggtgtgcga attcgcgcgt ttggttgcgt
     3961 ttcacaacga ggctgtgtca ccgctgccgc gcccgtcgag attgtcgacg tacccgctcg
     4021 tggctcgcgc tcgatcgaca gctatgtgag tgtcgggaga tgcgtctgac tgcggtcatc
     4081 ttgttgctct cggcatgctc gtggatcggc agcccggagc ggccggtcac gaactacacc
     4141 cagaagaccc agtcgtgctc gatggccgca ccgctcgcag atacgatcgc cgcgtctctg
     4201 ctgatcaccg gcggccttgc gatcgccgtt gcagacaacc tcgtcattcc ggacgtccag
     4261 tatcgcggcg cggcaacgct caagatcgca ctcccagcgg tcgtgatcgg tgccgtcttc
     4321 atcccatccg ccgtgcacgg ctggcgtgcg aagcggcgtt gcgaacatgc gctctcccaa
     4381 acgaaaacag gcgccgctct cgcgacgcct gctcgataag ctaagcgatc agaaactgat
     4441 tactggtctg ccgtgttcag cgagaacgac ccggcctcgt agccgtgcac accgatgaac
     4501 agcgtgccgt tgctcgtcgc ggtgtacgag acggtctcgt tgcctgacga ggtgtagccg
     4561 cggttcaggt acgcgtcggt cgtcggcgct gcgccccact ggatgtagag gtcgacgtcc
     4621 ttcgtcgacg tcgtgcggat cacgaccttc ttacccgccg gcatcggcag ctggaacatc
     4681 ttcatctcgc cttgcgtgac cgagccggtc tttgctaggt ggctgaacat cggcggcggc
     4741 gggacgacgc ctccgttgcc gttgccctcg gtgtagttga tcttgagcga aaaattgctt
     4801 gctgccgcgt agccgttgac ggcgacgtag acggtgcccg ggccgacgat cgagcactgc
     4861 tcttccgagc cgctgcgata cggacggcag tcgtacgatg ccgacgtcgg cgcggcgccc
     4921 ttgcggacgt agaggtcagc gtcgttgtcg ccggtcatcg acacggtgaa cgtcgtgccg
     4981 ccggcaacgt tgtacgggcc gtactgcttc cacgccgact tcacgactgc gccggtctcg
     5041 gtgaggttgc gaaccgggcc ggtggtgcca ccgttcggat ccgcgaccga ttggtcatag
     5101 atcatcttca cgttcgcgta ggtgatcttg ccgccggcga cgctggtcgc gctcgcgcgg
     5161 agcggcagcc acacgaagtc cgggtgctgg cgcttcgacg agccgaccca ctcaccaccg
     5221 acgatcttgc cggcgctgtc gacttcgaga acgtagtcgt agcgatcggt gtgcgtgtag
     5281 ttgttgatcg tcgagccgag gttgccatcg gtcgaggccg gcgactccga gatgtagtcg
     5341 acgtccatgt gcgtcttgta gagcttcgcg gcgttcgtgt tgaacacgta ggtcgtcggg
     5401 atcgagccgc cggcggtgat gttcagcgtg aagttcgccg cggtggtgcc gtacgcgttg
     5461 accgcgaggt acgcctgggt ctggccggcg ggcacggtga cgttgcacgt ctcggccggg
     5521 ccgctttcgt acggacggca gtcgtagctc gacgccgtcg gctgcgagcc gaacttgacg
     5581 tagaggtccg gatcgcccga gccgctcatc acgacggtga agttctggcc aggcgaaacg
     5641 gcgaccgtgc cgagctgcgt ccatgcgccg cctgcggcgg tgccggtctt cgtgctcgtc
     5701 gtgccgccct cggcgggaac gccgatcagc ttgttcgctt cgagcgccga gatttcggtc
     5761 gacgccgtga tccgatatcc gcgcagcggc tggttccaca cttcgccatc ccacgtgcgg
     5821 tcgtagacga atgcctcgcc ttgcttgccg aggtagttcg tcatgaggac gtggaacgtg
     5881 cccgggttcg tgtcccggca ctcggcgttc gtgttgttcg gacggccgta cttgtcgaac
     5941 gtgatctcgt tcgacgcgtc gagcgcgtcg caacgcagcg acacgaactt cgtgtcggtc
     6001 tgatcgtgaa cgagcgtgag cagggccttg atgtcctgca ccttgaactc gacgttgttg
     6061 tacgtgaccg cgtgcttggg ctccggcagc aggatcgcag ccggcgacca cgcgtggcag
     6121 atgccccacc acgtcggaat gcagcggcca ttcgtctgac cggtgcgctt cgcgcacgct
     6181 tcgccgagcg agctgttgca ctggctatcg gtcgtgcacg cggtacgcga cgcctgcgcg
     6241 tcgatgccgt ggtagcgcga gacgccgtcc tcgacgccgg tcacgccgaa cgcgcggccg
     6301 tacttggtcg atggcgcatc gctgctcgag ccattccact tgtagttgat gctgtcctcg
     6361 tagaccggcc agtagttacc ggcccacgga atgttgcggg cctcgccgac ctgcggcagc
     6421 tgcgccatcc ggtactcgag gttcgcgttg aacatggccg ggttgtccga agaaccccac
     6481 gcctctgcct taccttccgg tccgcctttg acgtcgctcg cgtccggctt gtcgcttccg
     6541 ttgtccacac agccggccac caagctcacg cccagggcaa aaccggccat ccaattcgtc
     6601 gccttcattt agtctcttct cccgatattt ctggcggcgc ccatgagcac ggaccgtgcc
     6661 ggctgtccga ccagtggccg gacggggtcg gacagcgcaa gccgttgttg cttctgcgtg
     6721 tcaaaaaccc aacgtcagcg aggacaaccg gccgctggct agggggctag ccgggacagc
     6781 gacgtccgcg cctcggtcgc cgactgcagc aggtgcggtg ccgggatcgg acgctgcacc
     6841 aggtgcagtc cgaggggccc aaaaaacgcg ccgtagaaaa ggtgagctag gagccgattc
     6901 caacgcttgt ccgacggagg caggccgcat agattgcccg cctgcccatg gggattgcag
     6961 atcttttccg ccccaagtat cgccacagcg acgtcaaggt tcgcaccgaa gccgtaaagg
     7021 ctttgaccca cgacgacgct gccatccttg ttcaagttgc tcggaccgac cgcgacattg
     7081 gcgtgcgccg tatcgcgatc gagaagatcg atgaagccga cgtgctcacg gagatcgcag
     7141 ctgcggagac cgaacgcagc ctgcgcgatt ttgccggtga gcgcgcggcc cagctgtgga
     7201 cgtcgcacgc gtgcagcgat gatgaggagg aggccaacga aggcctcgcc ggcatcacca
     7261 agctcggcga ccaacacgcg ctggccgaaa tcgcggtgaa cgcggcgctt cccgccgtgc
     7321 gcaaacgcgc gttcggcgac atgcgcgatc cgcgtgcgct ggccgagctc gcgaagggcg
     7381 atgccccgca agatttacgg ctcgcagccg tcggtcgcat cgatgacggc gacgtcctgc
     7441 gtgcgatcgc gatcgacacg acgctcaaag aggtcgggct cgcggcagtc gacaagctcg
     7501 acgacaccga ccggctcgag aacgtcgcac agaaggcgaa gaacaaagcg gtgcggcaac
     7561 gcgcgcgcaa gatcgtcgcg gagatggaag aagccgagcg cgcgaagagc aagccggcag
     7621 tccccgacga ggtgaagcgc cgccgcgccg agaaggcgca gctcgtccgc gaggtcgagg
     7681 gcgtcgccga ttcgttcgac ttcgagaagg tgagcgcgca ggtcaaggct gccgaggacg
     7741 cctgg
//
LOCUS       DASUFX010000262         5506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1570110, whole genome shotgun sequence.
ACCESSION   DASUFX010000262 DASUFX010000000
VERSION     DASUFX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570110"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFV99_10495"
     CDS             complement(<1..624)
                     /locus_tag="VFV99_10495"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5059779.1"
                     /translation="MRYELFYWPGIQGRGEIPRLILEDAGAEYMDVAREPGGMKRMQA
                     ILAGEEPALLPFAPPFLRAGNLWLAQSALIASFLGERLGLAPENESARLVARSIMLTL
                     ADFIDEIHDTHHPVAVEKYYADQKHEAALRATSFREARLPKFLRYLERNIDRSGHGVL
                     VGNAITYVDLAAFQIVDGLVYAFPQAFAKQHKHARGLLTLRDRVAQRP"
     gene            787..2571
                     /locus_tag="VFV99_10500"
     CDS             787..2571
                     /locus_tag="VFV99_10500"
                     /inference="COORDINATES: protein motif:HMM:NF006129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5059780.1"
                     /translation="MAQTVSDHIIQRLYSWGVRTIYGYPGDGINGLMGALNRCDGRVR
                     FIQTRHEEFASFMACGHAKFTGEIGVCMATSGPGAIHLLNGLYDAQMDHQPVVAIVGQ
                     AARSAIGADYQQEVDLVSLYKDVAHHYVHECTHPAAARHMIDRAMRIAFAERTVTCVI
                     IPNDVQELDGVDAPPREHNTTLSGIGYHRGIIVPGDYDLQMAAEILNSGKRVAMLVGA
                     GALGATDEVMAVAERLGAGVAKALLGKAVLPDDLPYVTGSIGLLGTMPSDRIMEHCDT
                     LLMIGSSFPYVEFLPKEGKARGVQIDLSPRMLSLRYPMDVSLHGDAAQTLRALLPLLE
                     EKKDRKWRGEIEGWVREWWKMMESRAMASADPINPQRVFWELSPRLPDNAILTADSGS
                     CAGWWARDLKIRPGMMASLSGNLATMGPALPYAAAAKFAHPDRPVVAAIGDGAMQMVG
                     NSVLVTVAAHYKEWSDPRLIVIVLNNHDLNQVTWEQRVMVGDPKFEVSQNLPPFPYAR
                     YANMLDLLGIEVATPDQIVPALEQAMRADRPVIIECHTDPEVPPLPPHITFKQAKNFM
                     ASIAHGDPNRWRMIEQSAKQVWAGINPK"
     gene            2576..3676
                     /locus_tag="VFV99_10505"
     CDS             2576..3676
                     /locus_tag="VFV99_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5059781.1"
                     /translation="MAKELAHIDRIDVRVCRIATEGGPETDGTATWDATTIVIVEARG
                     GGLTGIGYSYIDAAAAAVVRDLLAPAVIGVPLHATPAAMNRMLRAIRNHGRPGLVACA
                     ISAVDLALWDLKARVLAVPVASLLGPARRRVPVYASGGFTSESLEELDRELSEYAEQE
                     HVRVKIKIGRDAAHDLERVRVARDALGPDIELMVDANGAYARKQALAMADRFEQYDVV
                     YFEEPVSSDDLEGLRLMRDRAPAGMAIAAGEYGYDGWYFQRMLDAQAVDILQADATRC
                     LGATGFMQANALCAARSLPLSSHCAPSLHAHLDTAASQIVHLEYFRDHARVEHMLFDG
                     VPEVVAGAIAVDPVKPGFGLTLRETEVARHAA"
     gene            3666..>5506
                     /locus_tag="VFV99_10510"
     CDS             3666..>5506
                     /locus_tag="VFV99_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5059782.1"
                     /translation="MPHDRPVQLRRDRTDHEGSPLARALAKEIDGEVRFDRGTRALYA
                     TDASNYRQVPIGVVVPNTIEAVVATVALCHQHGAPIFARGGGTSLAGQTCNEAVVIDC
                     SRHLTGVSIDRASGTATVSPGTIMTSLRNAANAVGLDFGPDPSTKDRCTLGGMLGNNS
                     CGVHSVFSEFYGPGPRTSDHVEELEILTYRGQRMQVGATDALALHRARPEVAAIYERL
                     LALRDRYEPLIRSRYPDMPRRVSGYNLPDLLDDAGFHVGRALVGSESTCVMILGAKLR
                     LMRAKPQRVLAVLGYPDIYTAAAAANLARSRKPVGCEAMDDVLVNRIKVQRRDHEHAL
                     ALLPKGNAWLFVEHGADTLAEARAEAEMTARYLAPMATNILADHTSQQKLADMREEAL
                     GVDAFEMAQPDNYEGWEDSAVPPERLDHYLRELHALYKQYGLSGSLYGHFGQGCVHTR
                     IDFGLDRPEGVEVYRKFTYDAAHLVVAHGGSLSGEHGDGQSRGDLLEIMYGPELVDAF
                     REFKTIWDPDHAMNPGKVVDPNPRTSHLRLPDYHPAPQAIEFVHGDDGKNFAHAAVRC
                     VGIGKCRKRDAGTMCPSYMVTLDERHSTRGRAHLLFEMLHGDVIEDG"
BASE COUNT          932 a         1784 c         1857 g          933 t
ORIGIN      
        1 cggtcgctgc gccacgcgat cgcgaagcgt gagcaagcca cgcgcgtgct tgtgctgttt
       61 cgcgaacgct tgcggaaacg cgtagacgag cccgtcgacg atctggaacg ccgcaagatc
      121 gacgtacgtg atggcgttgc cgacgagcac gccgtgcccg ctgcgatcga tgttgcgctc
      181 gaggtagcgc aggaacttcg gtagccgcgc ttcgcgaaac gacgtcgcac gcagcgcggc
      241 ttcgtgcttc tggtcggcgt agtacttctc gaccgcgact ggatggtggg tgtcgtggat
      301 ctcgtcgatg aagtccgcga gcgtgagcat gatcgagcgc gcgacgagcc gtgccgattc
      361 gttctcggga gcgaggccga gtcgctcacc gaggaagctc gcgatcagcg cggattgcgc
      421 gagccagagg ttacccgcgc gcagaaacgg cggcgcgaac gggagcaggg caggttcctc
      481 gcccgcgagg atcgcttgca tgcgcttcat gccgcccggc tcgcgcgcga cgtccatgta
      541 ttcggccccc gcgtcctcga ggatcagcct cggaatctca cctcgccctt gaatgcccgg
      601 ccagtagaaa agctcgtagc gcatgcgcga accctagcgc gcagccgcgc gacgtcagcg
      661 tgagcactcg gcgaggaatg cgcgcccgcg tcgcgcacat tgcgacgcgt tcgctcgctc
      721 ctacgcgcta cttccacgcc gtcacaccgt gcgtacgggg catggcgggt gcacgtccgt
      781 cgatccatgg cgcagacggt gagtgatcac atcatccagc ggctctattc gtggggagtg
      841 cgtacgatct acggatatcc cggcgacggc atcaacggcc tgatgggcgc actcaatcgc
      901 tgcgatggtc gcgttcggtt catccagact cgccacgaag agttcgcgtc attcatggca
      961 tgcgggcacg cgaagttcac cggcgagatt ggcgtgtgca tggcgacatc ggggccgggc
     1021 gcgattcatc tgttgaacgg cctctacgac gcacagatgg atcaccagcc ggtcgtcgcg
     1081 atcgttggcc aggcggcacg cagcgcgatc ggcgcggact atcagcaaga ggtcgatctg
     1141 gtctcgctgt acaaggacgt cgcgcatcac tacgtccacg agtgcacgca tccagcagct
     1201 gctcgtcaca tgatcgaccg cgcgatgcgg atcgcgttcg ccgagcgcac ggtgacgtgc
     1261 gtgatcattc cgaacgatgt ccaggagctc gacggtgtgg acgcgccacc gcgcgagcac
     1321 aacacgacgt tgtcaggtat tggctatcac cgcggcatca tcgtgcccgg cgactacgac
     1381 ctgcagatgg ccgcggagat cctcaacagc ggcaagcggg tcgcaatgct cgtcggcgcg
     1441 ggcgcactcg gcgcgacgga cgaggtgatg gctgttgccg aacgactcgg cgccggcgtg
     1501 gcgaaggcgc tgctcggcaa ggccgtcctg cccgatgacc tgccgtacgt cacaggttcg
     1561 atcggtctgc tcggcacgat gccgagcgac aggatcatgg agcactgcga cacgctgctg
     1621 atgatcggct cgagcttccc gtacgtggag ttcttgccga aggaaggtaa ggcacgcggc
     1681 gttcagatcg atctgtcgcc gcgcatgctg tcgctgcgct atccgatgga cgtcagtttg
     1741 cacggcgatg cggcgcagac attacgcgcg ctgctgccgc tgctcgagga gaagaaagat
     1801 cgcaagtggc gcggcgagat cgagggttgg gtgcgtgagt ggtggaagat gatggagtcg
     1861 cgcgcgatgg cgtcggcgga tccgatcaat ccgcagcgtg tgttctggga gctctcaccg
     1921 cggctacccg acaatgcgat tctcaccgcg gactccggct cgtgcgccgg ctggtgggcg
     1981 cgcgatctga agatccggcc gggcatgatg gcgtcgctgt cgggcaatct ggcgacgatg
     2041 ggcccggcgc tgccgtatgc ggccgcggcg aagttcgcgc atccggatcg gccggtggtg
     2101 gctgcgatcg gcgatggcgc gatgcagatg gtcggcaaca gcgtgctggt cacggtcgcc
     2161 gcgcattaca aggagtggtc ggatccgcgc ctgatcgtca tcgtgctcaa caaccacgac
     2221 ctcaaccagg tgacgtggga gcagcgtgtg atggtgggcg atccgaagtt cgaggtgtcg
     2281 cagaacctgc cgccattccc gtacgcgcgc tacgcgaaca tgctcgacct gctcggcatc
     2341 gaggtcgcaa cgcccgacca aatcgtgcca gcgctcgaac aggcgatgcg tgccgatcgc
     2401 ccggtgatta tcgagtgcca caccgatccg gaagttccgc cgctgccacc gcacatcacg
     2461 ttcaagcagg cgaagaactt catggcgtcg atcgcgcatg gtgaccccaa ccgctggcgc
     2521 atgatcgagc agtcggccaa gcaggtgtgg gcgggcatca acccgaagtg atgccatggc
     2581 gaaagaacta gctcatatcg atcggatcga tgttcgggtc tgtcggatcg ccaccgaggg
     2641 tggtcccgaa accgacggca cggcgacctg ggacgcgacg acgatcgtca ttgtcgaggc
     2701 gcgcggtggc gggctgaccg gcatcggcta ctcgtacatc gatgctgcgg cggccgcggt
     2761 cgtgcgcgat ttgctcgcac cggccgtcat cggcgttccg ctgcatgcga ctccggcggc
     2821 gatgaaccga atgttgcgcg cgatccgcaa tcacgggcgt cccggcctcg tcgcgtgcgc
     2881 gatcagcgcg gtcgacctcg ccctctggga tctgaaggca cgcgtgctcg cggtgccggt
     2941 ggccagcttg ctcggtccag cgcgccgccg cgtgccggtc tacgcgagcg gtggcttcac
     3001 gtccgagagc ctcgaggagc tggatcgcga gctttccgag tacgccgagc aggaacacgt
     3061 ccgcgtcaag atcaagatcg gtcgcgacgc cgcgcatgac ctcgagcgcg tgcgggtcgc
     3121 gcgtgacgcg ctgggacccg acatcgagct gatggtcgat gcgaatggtg cctacgcgcg
     3181 caagcaagcg ctcgcgatgg ccgaccgatt cgagcaatac gacgtcgtgt atttcgagga
     3241 gccagtgtcg tccgacgatc tcgaaggtct gcgcctgatg cgcgatcgcg cgccggccgg
     3301 catggccatc gccgcgggcg agtacggcta cgacggctgg tacttccagc gcatgctcga
     3361 cgcgcaggcg gtcgatattc tccaggccga cgcgacgcgc tgcctcggtg cgaccggctt
     3421 catgcaggcg aacgcgctgt gtgccgcgcg gtcgttgcca ttgtcgtcgc actgcgcgcc
     3481 atctctccat gcccatctcg atacggccgc atcacagatc gtccacctcg aatacttccg
     3541 cgaccacgca cgcgtcgagc acatgctgtt cgacggcgtg cccgaggtag tcgccggtgc
     3601 gatcgcggtc gacccggtga agcccggctt tggcttgacg ctgcgcgaga cggaggtcgc
     3661 tcgccatgcc gcatgatcgt cccgtccagc ttcgtcgcga tcgcaccgat cacgagggca
     3721 gcccgctcgc gcgagcgctc gcgaaggaga tcgacggcga ggtgcgcttc gatcgcggca
     3781 cccgcgcgct gtacgcgacg gacgcgtcga attaccgcca ggttccgatc ggcgtggtcg
     3841 tgccgaatac gatcgaggct gtggtcgcga ccgtcgcgct ctgccatcag cacggcgcgc
     3901 cgatcttcgc gcgaggcggt ggtacgagcc tcgcgggcca gacctgcaac gaggcggtcg
     3961 tcatcgattg ctcgcggcac ctgaccgggg tgtcgatcga tcgcgcgagt gggacagcga
     4021 ccgtgagccc gggcacgatc atgacgtcac tgcgcaatgc agcgaatgcc gtcgggctcg
     4081 acttcggtcc cgatccgtcg acgaaggatc gctgcacgct cggcgggatg ctcggtaaca
     4141 actcgtgcgg cgtccactcg gtgttctccg agttctacgg ccccggtccg cgtacgtcgg
     4201 atcacgtcga ggagctcgag atcctcacgt atcgcggcca gcggatgcag gtgggggcga
     4261 ctgacgcgct cgcgctccac cgcgcgcgcc cagaggttgc agcgatctac gagcggctgc
     4321 tcgcgctgcg cgaccgttac gagccgctga tccggagcag atatccggac atgccgcgtc
     4381 gcgtgtcggg ctacaacctg ccggaccttc tcgacgatgc cggctttcac gtcggtcgtg
     4441 cgctcgtcgg cagtgagtcg acctgcgtga tgatcctcgg tgcgaagttg cgcctcatgc
     4501 gcgcgaagcc gcaacgcgtg ctcgccgtgc tcggctaccc cgatatctac actgctgcgg
     4561 cagcggcgaa cctcgcgcgc agccgcaagc ccgtgggctg cgaggcgatg gatgacgtgc
     4621 tggtcaaccg gatcaaggtg cagcgtcgcg atcacgaaca cgcgctcgct ctcttgccga
     4681 agggcaacgc gtggctcttt gtcgagcatg gcgccgatac gctcgccgaa gcgcgagcgg
     4741 aagccgaaat gaccgcgcgc tacctcgcgc ccatggcaac gaacatcctc gcggaccaca
     4801 cgagccagca gaagctcgcg gacatgcgcg aggaagcgct tggcgtcgat gcgttcgaga
     4861 tggcccaacc cgacaactac gagggctggg aagactccgc ggtgccgccc gagcggctcg
     4921 accactacct gcgcgagctg cacgcgctct acaagcagta cggcctctcc ggatcgctct
     4981 acggccactt cgggcaaggc tgcgtgcaca cgcgcatcga cttcgggctc gatcgtcccg
     5041 agggcgtcga ggtgtatcgc aagttcacat acgacgctgc gcacctcgtc gtcgctcacg
     5101 gtgggtcgtt gtccggcgaa catggcgatg gccagtcgcg cggcgacctg ctcgagatca
     5161 tgtacgggcc cgagctcgtc gacgcattcc gcgagttcaa gacgatctgg gatcccgacc
     5221 acgcgatgaa tccgggcaag gtcgtcgatc cgaacccgcg cacgtcgcac ctgcgcttgc
     5281 ccgactatca tccggcaccg caggcgatcg aattcgtgca cggcgacgat ggcaagaact
     5341 tcgcgcacgc tgccgtgcgt tgcgtcggca tcggcaagtg tcgcaagcgc gacgccggca
     5401 cgatgtgccc gagctacatg gtgacgctcg acgagcgcca ctccacccgc ggccgcgccc
     5461 acctcctgtt cgagatgctg cacggcgacg tgatcgagga cggctg
//
LOCUS       DASUFX010000263         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1708417, whole genome shotgun sequence.
ACCESSION   DASUFX010000263 DASUFX010000000
VERSION     DASUFX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708417"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VFV99_10515"
     CDS             <1..1196
                     /locus_tag="VFV99_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5059783.1"
                     /translation="RRMRTAKSTKGKPVPKTARAAAKPAVADKAKLKPKVEDASARGD
                     RSKRLLDVVMILLRARTPVTYRDIREQFPAYQTLNVEAGLRAFERDKADLLDLGVPIR
                     YVTPDEDDSLEDGGYVIDLKRFKMPEVHLTRDEISALVLAASVAHAMPGGTYPKIVDL
                     ALKKLAFDLPELPDTPTEFPRPANNAPTVLVHFPPKKHAPAGSDLGELGETYATLEAA
                     TRLRKRVSMRYQAATNGMISSRDLDPYAMVYREGAWLVVGWCHLRKEIRSFRVDRIHQ
                     AEMAPKPKSPDFERPADFDVKAYATRSPWTFTSEPPEEVQLALSAEAAEIANEDFGPT
                     AVKRQDGDRMLITFDCTNPEFAAMRVLAAKGAIHVTRGDKLRARIADELSAIGARYEV
                     EDVDA"
     gene            1189..2166
                     /locus_tag="VFV99_10520"
     CDS             1189..2166
                     /locus_tag="VFV99_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5059784.1"
                     /translation="MRDVAQRLRRLLFIVPYVAKHPEGVLVETLAKQIGAEREELLSD
                     LELLSQVGPPDGDPGEYLLVSVEEGRVFVDLAHRLTRPLRLTAAEGCSLLLGIRALRE
                     SGIAPFDSAMQSSERKLLAALGRDANEAQTLATSTVVAEPDAAVATHLRTLVSSARQR
                     QRVEIDYVSASRHQSEHRKVDPYGIVHHAGEWYFVGHCHNRGDVRTFRIDRVATLKTT
                     GERFDRPADFDLEAYRRERLYVPSADAVTVRVQLDPLAVTRVGANWPVGEVTLQDDGS
                     AELLVDCEGFEWVTGWVLNLGRHAWIVGPEEARKAMHDRVVRLRNELAA"
     gene            complement(2163..3203)
                     /locus_tag="VFV99_10525"
     CDS             complement(2163..3203)
                     /locus_tag="VFV99_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059785.1"
                     /translation="MKSRWIGVMVLALVACKGKEKPKEAVEPVAPAPAAVADAAQAAE
                     APPPAPTPTPPPAPSDQPPLSADVLPRDEATPKTRAVIADDFRFQIPESFTKAEQDGH
                     VVYTGTVEGFASPATMTFWASSEPFKGNLDALVKRETKAAADAKGEKPDVGPVMVFVA
                     DQVKQGYAKRLTIKFPDRIELRTVVAHDGKAFIMHCATPNVSNAWANVGSDCITRSTT
                     FNVAPLPLKPVKKPAGPPPPDVPNVKFMASKVEGTARQKTDVKPWLEDIAPFVKPCFA
                     SLEKGTSFKVSFEIAPDGAVGKSGVTDKDDKPADAKLAECAAKALATQKLDGKAAAAT
                     KVTMIFAVVEPF"
     gene            3321..>3973
                     /locus_tag="VFV99_10530"
     CDS             3321..>3973
                     /locus_tag="VFV99_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059786.1"
                     /translation="MRRLALLAPLVGLMIARAPTPAVACDDEDQEEAMDDVDVDVDVD
                     GPDDEDDNDDQFEAAIQAQIEQLQARIQEQVQRKLEEVARKLSRLHIDIDRDNDRADR
                     ERERAERARERAEQARERAMEQAERARERAMEAAERARERALQQADRVRQRTFRHGMQ
                     PILVPVPVPMVAPVPPVPPVPPVPPVPARARIAPPAPPAPPAPPPRFAPPVPQGLAPV
                     "
BASE COUNT          639 a         1407 c         1289 g          638 t
ORIGIN      
        1 cccgccggat gcggaccgca aagtccacaa aggggaaacc agtgccgaag actgcgcgcg
       61 cggcagccaa gcccgcggtg gcggacaagg ccaagctgaa gccgaaggtc gaggacgcga
      121 gcgcgcgcgg ggatcgcagc aagcgtctgc tcgatgtcgt gatgatcctg ctgcgtgctc
      181 gcacgccggt cacctatcgc gacatccgcg agcagtttcc cgcgtaccag acgctcaacg
      241 tcgaagcggg cctgcgcgca ttcgagcgcg acaaggccga cctgctcgat ctcggcgtcc
      301 cgatccgcta cgtcacgccc gacgaggacg actcgctcga ggacggcggc tacgtgatcg
      361 atctgaagcg cttcaagatg cccgaggtgc acttgacgcg cgacgaaatc tccgcgctcg
      421 tgctcgccgc atcggtcgcg cacgcgatgc cgggcggcac gtatccgaag atcgtcgacc
      481 tcgcgctcaa gaagctcgca tttgatctgc cggagctgcc cgacacgccg acggagttcc
      541 cacgccctgc gaacaacgca ccgaccgtgc tcgtgcactt ccctccgaag aagcatgcgc
      601 ccgcgggctc agatctcggg gagctcggcg agacctacgc gacgctcgaa gccgcgacgc
      661 gcctgcgcaa gcgcgtgtcg atgaggtacc aggccgcgac gaatggcatg atcagctcgc
      721 gcgatctcga tccgtacgcg atggtctatc gcgagggtgc gtggctcgtc gtcggctggt
      781 gccatctccg caaggagatc cggtcgttcc gcgtcgatcg aatccaccag gccgaaatgg
      841 cccccaagcc gaagtctccc gacttcgagc gaccggccga tttcgacgtg aaagcctacg
      901 cgacgcgctc gccgtggacg ttcacgagcg agccgcccga ggaagtgcag ctcgcgctat
      961 ctgccgaggc tgccgagatc gcgaacgagg acttcggtcc gacggcggtc aagcgccagg
     1021 atggcgaccg catgctgatc acgttcgatt gcacgaaccc cgagttcgcc gcgatgcgcg
     1081 tgctcgccgc aaagggcgcg atccacgtca ctcgcggcga caagctccgc gcgcgaatcg
     1141 ccgacgagct ctcggcgatc ggtgctcgct acgaagtgga ggacgtcgat gcgtgatgtc
     1201 gcccagcgcc tgcggcgcct gttgttcatc gttccgtacg tcgcgaagca cccggaaggc
     1261 gtgctcgtcg agacgctcgc gaagcagatc ggcgccgagc gcgaagagct gctctccgat
     1321 ctagagctgc tctcacaagt cggtccgccc gacggcgatc ccggcgagta cctgctggtt
     1381 tccgtcgagg aaggccgcgt gttcgtcgat ctcgcgcacc gcctcacgcg cccgctgcgg
     1441 ctcacggccg ccgagggctg ctcgctgttg cttggaattc gggctcttcg tgagagcggc
     1501 attgcgccgt tcgactcggc gatgcagtcg tcggagcgca agctgctcgc ggcactcggc
     1561 cgcgacgcga acgaagccca gacgctcgcg acgtcgacgg tcgtcgccga gccagatgct
     1621 gccgtcgcga cgcacctgcg cacgctcgtg tcgagtgcac gccagcgtca gcgcgtcgag
     1681 atcgattatg tctcggcgtc gcgccatcag tcggagcacc gcaaggtcga tccgtacggc
     1741 atcgtccacc acgcgggcga gtggtacttc gtcggccact gccacaatcg cggcgacgtc
     1801 cgcacgttcc gcatcgatcg cgttgcgacg ctgaagacca cgggcgagcg cttcgaccgc
     1861 ccggccgatt tcgatctcga agcgtaccgg cgcgagcgcc tctacgtgcc gagcgccgat
     1921 gcagtcaccg tccgcgtgca gctcgatccg ctcgcggtca cccgcgtcgg tgcgaactgg
     1981 cccgtcggcg aagtcacgct gcaggacgac ggctccgccg agctgctcgt cgactgcgaa
     2041 ggcttcgagt gggtgaccgg ctgggtgctc aacctcggcc gtcacgcctg gatcgtcggc
     2101 cccgaggaag cgcgcaaggc aatgcacgat cgcgtcgtgc gcctgcgcaa cgagctcgct
     2161 gcctagaacg gctcgacgac cgcgaagatc atcgtgacct tcgtggccgc ggcggctttt
     2221 ccgtcgagct tctgcgtcgc gagcgccttc gcggcgcact cggcgagctt cgcgtccgcg
     2281 ggcttgtcat ccttgtcggt gacgcccgac ttgccgacgg cgccgtcggg cgcgatctcg
     2341 aacgacacct tgaacgaggt gcctttctcc agcgacgcga agcacggctt cacgaacggc
     2401 gcgatgtctt cgagccacgg cttcacgtcg gtcttctgac gcgccgtgcc ctcgaccttc
     2461 gacgccatga acttcacgtt cggcacgtcc ggcggcggcg ggccggccgg cttcttcacg
     2521 ggcttgagcg gcagcggcgc gacattgaac gtcgtcgagc gcgtgatgca atcgctgccg
     2581 acgttcgccc acgcgttcga cacgttcggc gtcgcacaat gcatgatgaa cgccttgccg
     2641 tcgtgcgcga cgaccgtgcg cagctcgatc cgatccggga acttgatcgt gagtcgcttc
     2701 gcatagccct gcttgacctg gtccgcgaca aacaccatca ccgggccgac atcaggcttc
     2761 tcgcccttcg catctgcagc cgccttggtc tcgcgcttga cgagcgcgtc gaggttgccc
     2821 ttgaacggct cgctgctcgc ccagaacgtc atcgttgccg gtgacgcgaa gccttcgacg
     2881 gtgccggtgt aaacgacatg gccgtcctgt tcggccttcg tgaacgactc ggggatctgg
     2941 aaccggaagt cgtccgcgat caccgcgcga gtcttcgggg tcgcctcgtc gcgcgggagc
     3001 acgtccgcgg agagcggcgg ctgatcgctg ggtgcaggcg gaggcgtggg cgtcggagca
     3061 ggcggcggtg cctcggccgc ttgcgccgca tccgcgactg ccgccggggc cggggcgacg
     3121 ggctcgaccg cctccttcgg cttctccttc cccttgcagg ccacgagcgc gaggaccatc
     3181 actccaatcc atcgagactt cattgcgccc ttatatctcg actacttgcg cattctcagc
     3241 gcgacgttcg cgaaaacgcc ccgacgctgt catccgcctc gtcatctcgc ggctctaagc
     3301 gggaccagga ggatccatcc atgcgccgtc tcgccctgct cgcgccgttg gtcggtctga
     3361 tgatcgcccg cgctcccact cccgccgtcg cttgtgacga cgaagaccag gaagaggcca
     3421 tggacgacgt cgacgtcgac gtcgatgtcg acggccccga tgacgaggat gataacgacg
     3481 accagttcga agccgcgatc caggcgcaga tcgagcagct gcaggcccgg atccaggagc
     3541 aggtgcagcg caagctcgaa gaggtcgcgc gcaagctatc gcgcctccac atcgacatcg
     3601 atcgcgacaa cgaccgcgcc gatcgcgagc gcgaacgagc cgagcgcgcg cgcgaacgcg
     3661 ccgagcaagc acgcgaacgc gcgatggagc aggccgaacg cgcacgcgag cgcgcgatgg
     3721 aagccgccga acgcgcacgc gagcgcgcgc tgcagcaggc cgaccgtgtc cgccagcgca
     3781 cgttccgcca cggcatgcag ccgatcctcg tcccggtgcc ggtgccgatg gtcgcgcccg
     3841 tgccgccggt tccacccgtt cctccagtcc ctccggttcc cgcccgcgca cgcatcgcgc
     3901 cgcccgcgcc acctgccccg cccgctccgc cgccgcgttt tgcgccgccg gttccgcagg
     3961 gtctcgcgcc ggt
//
LOCUS       DASUFX010000264         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_41211,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000264 DASUFX010000000
VERSION     DASUFX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41211"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFV99_10535"
     CDS             complement(<1..1050)
                     /locus_tag="VFV99_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5059787.1"
                     /translation="MTTPKSDDAAPERDDGDDGDDVDLGLTSHLDADGKPRIVERHLI
                     VPPELAGLRLDHFVKTQIQRLSRTKIQQIIATQLRREDGFAPKPATSVAAGDHYIIRR
                     EARPEPPCPRTFGLLHEDARMRIIDKPAGLPVHASAKFYFNTLTRVLSERYPDEPELQ
                     LCHRLDRETSGCLVLARDKEAAAFLKGAIAAKDKTTKQYLAVVHGQPPWDSDNDTTLD
                     TSLRLSTAADPTRIPTVRVLPDPRGLTAITRVRVERRSASYALVRCTLVTGRQHQIRV
                     HLAVAGYPVVGDKIYAHGDDAFIDYCDEGLTPELAELFVLPRHALHAARITVPHPDGG
                     TVTAEAPLPSDMAALL"
     gene            complement(1031..2518)
                     /locus_tag="VFV99_10540"
     CDS             complement(1031..2518)
                     /locus_tag="VFV99_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5059788.1"
                     /translation="MKKFPDFVPPTKVSYLDRGPTLHLRRCILQGVDDPTAEWVFDKE
                     EIRIGSMEDNDVVLNDDTVSRYHCKIVQDDTGYVLVDNHSTNGTFINKVRVREGFLKP
                     GCQVAVGQSLLKFNAREEEVQIVPSRADRCGALIGANSKMREIYSIIEKIAPTATTVV
                     IDGETGTGKEVVAQSIHMLSPRGKNELVVFDCGAVPPNLIESELFGHEKGSFTGAMMT
                     RTGLFEQADGGTLFLDELGELPLDLQPKLLRALEQREVRRVGATKPNKVDVRIIAATN
                     RDLESEVRAGRFRQDLFYRLSVVRLHLPALRDRQDDIPLLVQHFLESGTYNRAQGGAS
                     RVRGVARDAMTALQNYPWPGNVRELVNVVERAVSFCSHEMIELSDLPDYVRTAKPQPI
                     KEQPAAKPLPRQPTGGNPTVGSALSPTPPQTPEELLNPGVTFKDAKERWVATFERDYI
                     LQMLRRNNGNISHAARAADIDRKYFRKLMKKYDIEAAGVDDDTEE"
     gene            2593..3066
                     /locus_tag="VFV99_10545"
     CDS             2593..3066
                     /locus_tag="VFV99_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059789.1"
                     /translation="MEGKKEGGPMATEKTATATEAKGTEKLKEKLKGRLDEARQRLEQ
                     AKTDIENLHAEDEAAVRAKAAEIRERIDSQKARAQSVKGQVSGWLREKQEQGESAISS
                     WRQKREIKRLEHRADKAEEYAVNAFVVAMMDADEAEMAVLEAFAARLDADAAYVQ"
     gene            complement(3140..3799)
                     /locus_tag="VFV99_10550"
     CDS             complement(3140..3799)
                     /locus_tag="VFV99_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059790.1"
                     /translation="MLWLALAACGREQYHVRLDPPRPGITPQERVQLFWQRRPTAEGT
                     VTTNDVETNKTLFLGDKQKGVEQVEVVSPEDLEPLVGADSETMEHARRSVHARNRSNT
                     AWYASIVVLAGGFVLGIAVDRNTSDSGLPWTWITFGASAGTAVLLWSVERHWARDELE
                     WRKRAFETYTRDLGQLLNVCAHGMEVVPCEAPINAPSDPSQPKQPATSIPGRTALLRM
                     R"
     gene            complement(3808..4443)
                     /locus_tag="VFV99_10555"
     CDS             complement(3808..4443)
                     /locus_tag="VFV99_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059791.1"
                     /translation="MRVFLCFALLLIACGEKPHVNIAAPPPNLTPDQRVDAFNKLRTE
                     ATGVEVVTTCNHGCTSTSTPLLRIGAGNVVRYAEDLLPVLPQGSPAAAAAHDVETARH
                     KAKRVSRIGGLVFAAGFVTMMLAFNYGDELALIGGATMAGGLIGWAYGVYGYDREIKE
                     KTKLVFNSYDESLATTFNVCVNGMNVVACEQSTPGTTPQEPDPALKALRQK"
     gene            complement(4535..5167)
                     /locus_tag="VFV99_10560"
     CDS             complement(4535..5167)
                     /locus_tag="VFV99_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5059792.1"
                     /translation="MLAKVGIIVSALLLSSVALADKHKNIDSTTGPDFVEATPPRHIP
                     RGLVASPDAAKPIDPWAVVPFELDKARLTAEGYDDVDRASRWLYSHPKHQIILEGHAD
                     AAGRVPYNDDLATRRMEAVRNRLIRNGIPNDRIVTITFGERESMDVKNPLHGADRKVV
                     MYATTLSPQAVIAMVQQNRPAVFAAYSEYGQLMRVEQGLGQPARTITVRR"
     gene            complement(5310..>5491)
                     /locus_tag="VFV99_10565"
     CDS             complement(5310..>5491)
                     /locus_tag="VFV99_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059793.1"
                     /translation="EQLAGGTDERAAYDIFPITRNFSDQHHARRCGSFAEHGLRGVLV
                     QRAASARWDRSAQCR"
BASE COUNT          920 a         1721 c         1852 g          998 t
ORIGIN      
        1 caacagtgca gccatatcgc tgggtaacgg tgcttccgcc gtcacagtgc cgccgtcggg
       61 gtgaggcacg gtgatgcggg ccgcgtggag cgcgtggcgc ggcaacacga acagctccgc
      121 aagctctggg gtgaggcctt cgtcgcaata atcgatgaac gcgtcgtcgc cgtgcgcgta
      181 gatcttgtcg ccgacgaccg gatagccggc cacagcgagg tggacgcgga tctgatgctg
      241 gcggccggtg acgagcgtgc agcggacgag cgcgtagctc gccgatcgcc gttcgacgcg
      301 cacgcgagtg atcgcggtga gcccgcgcgg atcgggcaac acgcgcacgg tcggaatcct
      361 cgtcgggtcg gcagccgtcg acaggcgcag cgaggtgtcg agcgtcgtgt cgttgtccga
      421 atcccacggc ggctgaccgt ggacgaccgc gaggtactgc ttcgtcgtct tgtccttcgc
      481 cgcgattgca cctttgagaa acgcggccgc ctctttatcg cgcgcgagca cgaggcagcc
      541 gctcgtctcg cgatcgaggc gatgacacag ctgcagctcg ggctcgtcgg ggtagcgttc
      601 ggagagcacg cgcgtgagcg tgttgaaata gaacttcgcg gacgcatgca cgggcagacc
      661 tgcgggtttg tcgatgatgc gcatgcgcgc gtcctcgtgg agcaggccga acgtgcgcgg
      721 acacggcggc tcgggacgcg cttcgcggcg aatgatgtag tgatcgccgg ccgcgacgct
      781 cgtcgctggc ttcggcgcga agccgtcttc gcggcgcagc tgggtcgcga tgatctgctg
      841 gatcttcgtc cgcgacagtc gctggatctg cgtcttcacg aagtgatcga ggcggagccc
      901 tgcgagctcg ggcggcacga tcagatggcg ctcgacgatg cgcggcttgc cgtcggcatc
      961 gagatgcgac gtcagcccaa gatcgacgtc gtcgccatca tcgccgtcgt cacgctcggg
     1021 ggccgcgtcg tcactcttcg gtgtcgtcat cgacgcccgc agcctcgatg tcgtacttct
     1081 tcatcagctt gcggaagtac ttgcggtcga tatcggccgc gcgtgccgcg tgggagatgt
     1141 tgccgttatt gcgacggagc atctgcagga tgtagtcgcg ctcgaacgtc gcgacccaac
     1201 gttccttcgc gtccttgaac gtgacgccag gattgagcag ctcttcgggc gtctgcggcg
     1261 gcgtcggtga cagcgccgag cccaccgtcg gattgccgcc ggtcggttgg cgcggcagcg
     1321 gcttggcggc cggctgttcc ttgatcggct gcggcttcgc ggtgcgcacg tagtccggca
     1381 gatccgacag ctcgatcatc tcgtggctgc agaagctgac cgcccgctcg acgacgttga
     1441 cgagctcgcg cacgttgccg ggccacggat agttctgcag cgcggtcatc gcatcgcgcg
     1501 cgaccccgcg cacccgcgac gcgccgccct gcgcacggtt gtacgtgccg ctctcgagga
     1561 agtgctggac gagcagtgga atgtcgtcct gacgatcgcg cagcgccggc aagtgcaggc
     1621 gcacgacgct caggcgatag aacagatcct ggcggaagcg accggcgcgc acttcgctct
     1681 cgaggtcgcg gttcgtcgcc gcgatgatgc ggacgtcgac cttgttcggt ttcgtcgcgc
     1741 cgacgcgccg cacctcgcgc tgctcgagcg cgcggagcag cttcggctgc aggtcgagcg
     1801 gcagctcgcc gagctcgtcg aggaacagcg tgccgccatc ggcctgctcg aacagaccgg
     1861 tgcgcgtcat catcgcgccg gtgaagctgc ccttctcgtg accgaacagc tcgctctcga
     1921 tcaggttcgg cggcacagca ccgcaatcga acacgacgag ctcgttcttg ccgcgcggcg
     1981 acagcatgtg gatcgactgc gcgaccactt ccttgccggt gccggtctcg ccgtcgatca
     2041 cgaccgtcgt cgcggtcggc gcgatcttct cgatgatcga gtagatctcg cgcatcttcg
     2101 agttcgcacc gatcagcgcg ccgcagcggt ccgcgcgcga cgggacgatc tggacctctt
     2161 cttcgcgcgc gttgaacttg agcaggctct ggccgaccgc gacctggcag ccgggcttca
     2221 ggaagccctc gcggacgcgc accttgttga tgaacgtgcc gttcgtcgag tggttgtcga
     2281 cgagcacgta gccggtgtcg tcctggacga tcttgcagtg gtagcgcgac accgtgtcgt
     2341 cgttgaggac gacatcgttg tcttccatcg agccgatgcg gatctcttcc ttgtcgaaga
     2401 cccactcggc cgtcggatcg tcgacgccct gcaggatgca acgccgcaga tgcaacgtcg
     2461 gcccgcggtc gaggtaactg accttggtcg gcggaacgaa gtcagggaac ttcttcatga
     2521 aacccgcgcg gccatcatac tcgaaatggc gttttgtcgc tcgaagcacg cgttatgcat
     2581 ggatcttggc gcgtggaggg gaagaaagag ggcggtccaa tggcgacgga aaagacagca
     2641 actgcgacgg aagcgaaggg aaccgagaag ctaaaggaga agctgaaagg gcggctagac
     2701 gaagcaagac aacgtttgga acaggcgaag accgacatcg agaacctcca tgccgaggat
     2761 gaggcggcgg ttcgcgcgaa ggcggccgag atccgcgagc gcatcgactc gcagaaagcg
     2821 cgggcgcaga gcgtgaaggg ccaggtgtcg ggctggctcc gcgagaaaca ggagcagggt
     2881 gaatcggcga tcagctcgtg gcgacagaaa cgcgagatca agcggctcga gcatcgcgcc
     2941 gacaaggccg aggaatacgc ggtcaacgcg ttcgtcgtgg cgatgatgga tgcggatgag
     3001 gccgagatgg cggtgctaga agcattcgcg gctcggctcg atgccgatgc ggcttacgtt
     3061 cagtaagccg cgaagcggcg cacgacaaac actgtgcacc gcgggcgtag cgatgggcgc
     3121 gtcagatgaa tggcgagtgc tagcgcatcc gcaacagggc ggtgcgacca gggatgctcg
     3181 tcgccggctg tttcggttgg ctcgggtcac tcggcgcatt gatcggcgcc tcgcatggga
     3241 cgacttccat gccatgcgca cacacgttca agagctgccc gagatcgcgc gtgtatgttt
     3301 cgaacgcgcg cttgcgccac tcgagctcat cgcgtgccca gtgcctctcg acgctccata
     3361 acagcaccgc ggttccggcg ctggcgccga atgtgatcca tgtccacggc agacccgagt
     3421 cggacgtgtt gcggtccacc gcaatgccga gcacgaagcc gcccgcgagc acgacgatcg
     3481 acgcgtacca ggcagtgttc gatctgttgc gcgcatgcac gctgcgccgc gcatgctcca
     3541 tcgtctcgct gtcggcaccg acgagcggct cgagatcctc gggcgacacg acctcgacct
     3601 gctcgacgcc cttttgcttg tcgccgagga acagcgtctt gtttgtctcg acgtcgttcg
     3661 tcgtgacagt gccctctgcg gtcgggcgac gctgccagaa cagctgcacg cgttcctgtg
     3721 gtgtgatgcc aggccgcggc gggtcgaggc gcacgtggta ctgctcgcgg ccacacgctg
     3781 cgagtgcgag ccagagcatg cactccctca tttttgcctc aacgctttga gcgccggatc
     3841 gggttcctgc ggtgtggtac cgggcgtgct ctgctcgcag gcgaccacgt tcatgccatt
     3901 gacgcagacg ttgaacgtcg tcgccagact ctcgtcgtag ctgttgaaga cgagcttggt
     3961 cttctccttg atctcgcggt cgtagccgta cacgccgtag gcccagccga tgagtccgcc
     4021 ggccatcgtc gcgccgccga tgagcgcgag ctcgtcgccg tagttgaatg cgagcatcat
     4081 cgtcacgaaa ccggcggcaa acacgaggcc gccgattcgc gacacacgct tggccttgtg
     4141 gcgcgctgtc tcgacgtcgt gtgccgcagc ggccgcgggg ctaccttgtg gcagcaccgg
     4201 cagcaggtct tcggcgtacc gcacgacgtt gcctgccccg atccgcagca atggtgttga
     4261 tgtgctcgtg cagccgtgat tacaggtcgt gacgacctcg acgccggtgg cttccgtgcg
     4321 gagcttgttg aatgcgtcga cccgctggtc gggggtgagg ttcggcggcg gagcggcgat
     4381 gttgacgtgt ggcttctcgc cgcaagcgat caacaacaga gcgaagcaca agaagacgcg
     4441 cacgccgaac atgatgcatt agcgtcggca tcatttgaat gtgggcacgc gtcgtaccgt
     4501 gcgtgtgcgc acggcgtcgc aaccgattgc tgcgtcagcg acgaaccgtg atggtccggg
     4561 ccggttggcc gagtccctgc tcgacccgca tcaactggcc gtactccgag tacgcggcaa
     4621 agaccgcggg acggttctgc tggaccatcg cgatcacggc ctgcggcgag agcgtcgtcg
     4681 cgtacatcac gactttgcgg tcggcgccgt gcagcgggtt cttcacgtcc atcgactcgc
     4741 gttcgccgaa cgtgatcgtc acgattcgat cgttcgggat gccgttgcga atgagacggt
     4801 tgcgcaccgc ttccatgcga cgcgtcgcga ggtcgtcgtt gtacggcacg cgaccggctg
     4861 catccgcatg gccctcgagg atgatctgat gcttcggatg gctatagagc cagcgcgatg
     4921 cgcggtcgac gtcgtcgtag ccttccgccg tcagccgtgc cttgtcgagc tcgaacggca
     4981 ccaccgccca cggatcgatc ggctttgcag cgtcgggcga cgcaacgagg ccacgcggga
     5041 tgtgacgggg cggcgtcgcc tcgacgaaat caggtccggt cgtgctgtcg atgttcttgt
     5101 gcttgtctgc gagcgcgacc gacgacagca gcaacgcact tacgatgatt cctactttcg
     5161 ccaacatgct ccaaggaatc agcatctcgt gtgccagcgg cacgtgatgt gcttcaccgc
     5221 tcatcatgtc gctcgttcgc gcacgccaac caaaaccgct acctgacgac gtgccgggcg
     5281 ctgagggatt cctccccgag cggcatacgt taccgacact gcgcgctgcg atcccaacgt
     5341 gccgaggctg cgcgctgtac aagcacgcca cgcaggccgt gttcggcgaa ggatccgcag
     5401 cgtcgcgcgt gatgctgatc ggagaagttc cgggtgatcg ggaagatgtc gtaggccgcc
     5461 cgttcgtcgg tcccgccggc aagctgctcg a
//
LOCUS       DASUFX010000265         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_41677,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000265 DASUFX010000000
VERSION     DASUFX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41677"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFV99_10570"
     CDS             complement(<1..441)
                     /locus_tag="VFV99_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059794.1"
                     /translation="MKFPASIADAVSPANRSKLRFGIYTGYALAAAFAVLAVAASALD
                     LTPFRWAFVGLISTKVLTNSLAWLALVRDRFVAITQVLNTTADVVLLTGAIYFTGGSY
                     SPLLATYVIIISAIALLANLGVTIIMALAILIIYSTMMILMATGV"
     gene            complement(506..877)
                     /locus_tag="VFV99_10575"
     CDS             complement(506..877)
                     /locus_tag="VFV99_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059795.1"
                     /translation="MKKILVVEDDPVNQMILMDFLAANGYQTIAAASGPEGIEQFDRT
                     SPDLCLVDVQLPRKNGFELVREIKSRGTQTPVMLMSAVYTDQDQSTRTVQLGALADGY
                     LTKPFDLVQLLAQVRQLLGEA"
     gene            complement(895..2136)
                     /locus_tag="VFV99_10580"
     CDS             complement(895..2136)
                     /locus_tag="VFV99_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059796.1"
                     /translation="MTDRDELAAIKALITSGDDRAAFARVRARLGWPRGKELTDVAAW
                     LEVLGHLAAGRGAGELADVAAAAVRDPDSPDHLYDLGYALIDAGAPSIAASVLWRCLS
                     LVGDSEEVVCELVSALESALAYNDAFAILEQHASLRNRSFLCEYLYAFNAAMTGRLDT
                     TRTVLARLTPDSPETESMKATITAITERADRIAGVSSLDERDLRGWQYVLTGTLVAHQ
                     SPYGFEQPMHGRYAWLQDSIGRVATGIARLAPLVRALHVPCVYAPPGRNHEILARAVS
                     RMVDLPIAPWPAVGVPAPGLVVVYDLAELPAQDIQRLQQRKPDQIVYAHASPWTQDFP
                     IAPDVTTVLYQIIAAPWDGERTVEDVVAEIASSPGLDTEETSADDVTRWATLCEKAWP
                     PLPGQRARLWAGGPVPSNRFE"
     gene            complement(2133..2954)
                     /locus_tag="VFV99_10585"
     CDS             complement(2133..2954)
                     /locus_tag="VFV99_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5059797.1"
                     /translation="MADRVALVTGSTDGIGRQTARQLAAKGIKVIVHGRSKQKVDATI
                     AWLKEELPGGEFDGVSFDIGRLAEVRRGAGQVLELAPKLHVLVNNAGIFANERVVTSD
                     DIETTFAVNYLGPFLLTELLGPRLTDSAEGMASRVINLSSVAHTRGRIHFNDLQLASS
                     WTGYAAYAQSKLAQVMHALTLADRHEPSKLLAYSVHPGVVGTKLLRQGFGPVQGIAPD
                     AGARTSVMLASGANEDEPSGSYYSDGVATQPSSAARDADAREKLWNLSVQLAKIG"
     gene            complement(2964..>3823)
                     /locus_tag="VFV99_10590"
     CDS             complement(2964..>3823)
                     /locus_tag="VFV99_10590"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059798.1"
                     /translation="DDVDGTVPYVAPISFGAPDARYNPFLDTLGTDECRNQVRAIATD
                     MLANRRDAMLAKAQAQAAANNFYYNRVPIGPAVESAIFNLEWAFWQYFGVGQCATVPA
                     VTTDDNVMWKFLDDISPVSDNSDARIDQFDAYYYQAYNQLGYPDGGAMYLDPYLQYTD
                     ADYLNALPTAQPAYDDAAAMLDVDKFVREQGDRFLFIYGQWDPWTGGQFELGRAKDSL
                     LLIQPEGTHGSRLTKLTDADREAAFAKLEAWTGITPQIPPGRSSREFAPPHVPSAFSR
                     ALRARKLSP"
BASE COUNT          663 a         1233 c         1275 g          652 t
ORIGIN      
        1 cacgcccgtc gccatcagga tcatcatcgt cgagtagatg atcaggatgg cgagcgccat
       61 gatgatcgtg acgccgaggt tcgcgagtag cgcgatcgcc gagatgatga tcacgtacgt
      121 cgcgagcagc gggctgtacg agccgccggt gaaatagatc gcaccggtga gcagcacgac
      181 gtcggcggtc gtgttgagca cctgcgtgat cgcgacgaac cgatcgcgga ccaacgcgag
      241 ccaggcgagc gagttcgtga gcaccttcgt cgagatcagg ccgacgaacg cccaccgaaa
      301 cggtgtgagg tcgagcgccg acgcggctac ggcgagcacg gcgaacgctg ccgcgagcgc
      361 atagcccgtg tagatgccaa accgcagctt cgaccggttg gccggcgaaa cagcgtccgc
      421 gatcgacgcg gggaacttca cccgcgtatc ttagtccatc agacccgcgt acttagcggt
      481 tggggtcagg cactacttgt actacttacg cttcgccgag cagctggcgg acctgggcga
      541 gcagctgcac gaggtcgaag ggcttggtca aatagccgtc cgcgagcgca ccgagctgga
      601 ccgttcgggt cgactgatcc tggtcggtat agacggcgct catcaacatg accggcgtct
      661 gggtgccgcg gcttttgatc tcgcggacga gctcgaaccc gttcttgcgc ggcagctgca
      721 cgtcgacgag gcacaggtcg ggtgacgtgc gatcgaactg ctcgatgccc tccggtcccg
      781 acgccgccgc gatggtctgg tagccgttgg ccgccaggaa atccatcagg atcatctgat
      841 tgaccggatc atcctcgacg acgaggatct tcttcacgtc gtcgagcgta gcacttattc
      901 gaaacggttc gatggcaccg gaccgccagc ccacaaccgt gcacgttgac ctggtaacgg
      961 cggccacgct ttttcgcaaa gcgttgccca acgtgtgacg tcgtcggcgg atgtctcctc
     1021 ggtatcgaga ccggggctcg acgcaatttc cgcgaccacg tcctcgacgg tgcgctcgcc
     1081 gtcccagggt gcggcgatga tctgatagag caccgtcgtc acgtccggcg cgatcggaaa
     1141 gtcttgcgtc cacgggctgg cgtgcgcgta gacgatctga tccggcttgc gctgctgtag
     1201 gcgttgtatg tcttgcgcag gcaactctgc gaggtcgtac acgaccacca acccgggcgc
     1261 cggaacgcca accgcgggcc acggagcgat cggcagatcg accatccgcg agaccgcacg
     1321 tgcgaggatc tcgtgattgc ggcccggcgg cgcgtagacg cagggcacgt gcaacgcacg
     1381 cacgagcggc gccagtcgcg cgatcccggt ggcgacgcgc ccgatcgagt cttgcagcca
     1441 cgcatagcgg ccgtgcatcg gctgctcgaa gccgtatggc gattgatgcg caaccaacgt
     1501 gccggtcagt acgtactgcc agccgcgcag atcgcgctcg tcgagcgagc tgacgccggc
     1561 gatgcgatcc gcgcgctcgg tgatcgccgt gatcgtcgcc ttcatcgact cggtctcggg
     1621 cgaatcgggc gtgagccggg cgagcacggt gcgcgtcgtg tccagccgcc cggtcatggc
     1681 ggcgttgaac gcgtagaggt actcgcacaa gaagctgcga ttgcgcagcg acgcgtgctg
     1741 ctcgaggatc gcgaacgcat cgttgtacgc gagcgcgctc tcgagcgccg atacgagctc
     1801 gcagacgact tcctcgctgt caccgacgag cgacaggcac cgccagagca cgctcgccgc
     1861 gatcgagggc gcgcctgcat cgataagcgc atagccaaga tcgtatagat gatccggact
     1921 gtccggatcg cggactgctg cggcggcgac atcggccagc tcaccggcgc cgcgccccgc
     1981 cgcgaggtgc ccgagtacct cgagccatgc cgcgacatcc gtgagctcct tgccgcgcgg
     2041 ccagcccagt cgagctcgga cccgagcaaa cgctgcgcga tcatcgccgc tggtgatcag
     2101 cgctttgatc gcagcgagct cgtctcggtc agtcatccga ttttggcgag ctgcacggaa
     2161 aggttccaca gcttctcgcg agcatcggca tcacgcgcgg cgctcgacgg ttgcgtggcg
     2221 acgccgtcgc tgtaatagct tcccgacggt tcgtcttcgt tcgcgccgct cgccagcatc
     2281 accgacgtgc gcgcgcctgc gtccggcgcg atgccctgca ctggcccgaa gccctgacgc
     2341 aacaacttcg tgccgacgac acctggatgc acgctgtacg cgagcagctt cgacggctcg
     2401 tggcgatcgg caagcgtgag cgcgtgcatg acctgcgcga gcttcgactg cgcgtatgcc
     2461 gcgtaaccgg tccacgagct cgcgagctgg aggtcattga agtgaatgcg accgcgcgta
     2521 tgcgcgaccg acgacaggtt gatcacgcgc gacgccatgc cttcggcgct gtcggtgagc
     2581 cgcggaccga gcagctcggt gagcaagaac ggcccgaggt agttcacggc gaacgtggtc
     2641 tcgatgtcat ccgacgtcac gacgcgctcg ttcgcgaaga tgccggcgtt gttgacgagc
     2701 acgtgtagct tcggcgcgag ctcgagcacc tgaccggcgc cgcggcgaac ctcggcgagt
     2761 cggccgatgt cgaacgacac gccatcgaat tcgccgcccg gcagctcttc cttcagccac
     2821 gcgatcgtcg cgtcgacctt ctgcttgctg cggccgtgaa cgatgacctt gatgcccttc
     2881 gcggcgagct gacgcgcggt ttgcctcccg ataccgtcgg tggaaccggt gacaagcgct
     2941 acgcgatcgg ccatgcgccg actctacggc gacaacttgc gggcgcgcaa tgcacgactg
     3001 aaggccgacg ggacgtgggg cggcgcaaat tcccgggatg accgacctgg cggaatctgc
     3061 ggcgtaatcc ccgtccacgc ctcgagcttt gcgaaggcgg cctcacggtc ggcatcggtc
     3121 agcttggtga gccgcgagcc atgggtgccc tcgggctgga tgagcagtag cgagtctttc
     3181 gcacggccga gctcgaactg gccgcccgtc cacggatccc actggccgta gatgaacagg
     3241 aaccgatcgc cctgttcgcg cacgaacttg tcgacgtcga gcatcgccgc ggcgtcgtcg
     3301 tacgcaggct gcgcggtcgg cagcgcgttc aagtaatccg catcggtgta ctgcagatac
     3361 ggatcgaggt acatcgcgcc accgtcgggg taaccgagct ggttgtacgc ctggtagtag
     3421 tacgcgtcga actggtcgat acgcgcgtcg ctgttatccg agaccggcga gatgtcgtcg
     3481 aggaacttcc acatcacgtt gtcgtcggtg gtgaccgctg gcaccgtcgc gcattgcccg
     3541 acgccgaagt actgccagaa cgcccactcg aggttgaaga tcgcggactc gacggcgggg
     3601 ccgatcggga cgcggttgta atagaagtta ttcgcggcgg cctgcgcctg cgccttcgcg
     3661 agcatcgcat cgcggcgatt cgcgagcatg tcggtggcga tggcgcgcac ctggttgcgg
     3721 cactcgtcgg tgccgagcgt gtcgaggaac gggttgtagc gcgcgtcggg cgcgccaaac
     3781 gagatcggcg cgacgtacgg caccgtgccg tcgacgtcgt cgg
//
LOCUS       DASUFX010000266         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_876155,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000266 DASUFX010000000
VERSION     DASUFX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876155"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFV99_10595"
     CDS             <1..472
                     /locus_tag="VFV99_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059799.1"
                     /translation="AGSTQFSIDLGFPFRYAIKPEIAIVALQTLMQIDFNEAKTDHVT
                     VKSETDPATMMTRITSENTGDGAKPDLNPSIGIATNPVPPLSVVVYAQLRIPDFDTSA
                     GAFQVPVTGRIEFSPNQKFDVGLEFTLLNVKPPDGQSPIENRFLALFVQSRFGK"
     gene            469..1722
                     /locus_tag="VFV99_10600"
     CDS             469..1722
                     /locus_tag="VFV99_10600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059800.1"
                     /translation="MKVCPRCFTRFPDGERFCLHDSAVLVEEEDIARLGHNIGNYRLD
                     KILGRGGMGTVYMGEHIYIKKPVAVKVLHPQFARYQEAIHRFLREARAATSINHPNIV
                     DVTDFGILPEGPVYFVMEFLEGKSLEDLIEKEGAVELHRALNIANQIALALDAAHAVG
                     VIHRDLKPDNIMLLKRPGRRDLVRMTPDQNWITEREESYDFVKVLDFGIAKVLIQDEL
                     MAETVQGAVFGTPEYMSPEAARGEDVDHRADVYSLGVILFDMLCGRPPFEAPQSSEVL
                     AMHIHSPPPSPREFAPHREITEQAEAVILKAMQKDPARRYQNMVEFRRDLEISYGSIS
                     YRRRGAPGGRIEPRGMEARAPKKRLTEELDEWITNDPSGMSVEQARMMSLVETAEKAF
                     TPTGMSPAEAERIANALDRALDDDE"
     gene            1796..2038
                     /locus_tag="VFV99_10605"
     CDS             1796..2038
                     /locus_tag="VFV99_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059801.1"
                     /translation="MSANQANQSGQITLRKDVKFNGVKVFSATMVADRDQLGEKVTAW
                     IQANKEKKVTDIVITQSSDEAFHCIAITVFYWEDRT"
     gene            2035..3096
                     /gene="serC"
                     /locus_tag="VFV99_10610"
     CDS             2035..3096
                     /gene="serC"
                     /locus_tag="VFV99_10610"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX5059802.1"
                     /translation="MRKLNFSSGPAVLPEAVLRRAQQAIWDLDGSGIGILEHSHRGKE
                     FGRVLDRAEALVREVGGIGDEFAILFMTCGATQHFALAPQNFLGVGVTADYCHTGIWS
                     GKAMEDAKRYGSVHVACSAEADNFASVPTSFSWSASPRYVHYTSNETIYGTEWQSPPK
                     APAPLVCDASSDIFSKPLVLDGHAMIYAGAQKNLGPSGISLVIARRDFIATGTKDLPA
                     LYQYRTYEAERSMHNTPNTFGIWMIAEVCAWIKEQGGLAAMAEHNRKKAQLLYDALDA
                     SKVWRTHAKPGSRSLMNVTFRGATKEIEEQLIARAEERGLSGLRGHRSVGGMRASLYN
                     AFPEDGVRKLVDLLHELER"
     gene            3093..3740
                     /locus_tag="VFV99_10615"
     CDS             3093..3740
                     /locus_tag="VFV99_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059803.1"
                     /translation="MKFLTAAGQRAIEDAVAAIEAVSSAEIVIAVRPRARHALMQHAT
                     VGIVAAIAILAFTLYSPIEFSLWQILVLPIFAGILGALLVEAIPPLYRFVEPPWLRYE
                     HVREAAYAAFVEKRVHATRDRTGVLIYVAVRERMVEVVGDLAVADKLGIEGIAAMAGT
                     LEAFVPRGSEAFGKALAGQVDEFAKALPRRADDIDELSNAVTVFGAPPRQRKAVS"
     gene            3737..>5134
                     /locus_tag="VFV99_10620"
     CDS             3737..>5134
                     /locus_tag="VFV99_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX5059804.1"
                     /translation="MSWPPRRAALLLGLFVVGALAGVAAARPGGGQSYSGGGHSYSGG
                     GGYSSGHSYSGGSYSSSNSGGGSLSAGAALGILFGFAIMIVIIAVVQGAQSNAYTSAL
                     TAPTPPPRLDLQPLLSRDPDFSRVAFEDFAYRLYSESQRARHARDELIKLAPYLGAGA
                     FDGLLRRASRVEQVVIGNLRIIQVGAELAGTRTKVVVAIEANLAGPSGTTNALERWTF
                     LRAPNVTTRPPERTRTFACPNCNAPFASGETWRTCAHCGTDIETGKFDWTVDAIAVDA
                     ETSVGQTLTGTVEEVGNDFPTLIEPEAQERLMQLRQDDTAVTWDAFTARVAMIYARLN
                     SAWNAQELAPVRGLVTASLLQYLQYWIDEYKRQTFANKLEEARISHIALAKVTRDKWF
                     DAITVRVYADGLDFTVDKEQRVVGGSRSNRRAYTEYWTFIRSSARRGPVTVDPKCPNC
                     GAPLAISNAGVCTHCN"
BASE COUNT          977 a         1646 c         1640 g          871 t
ORIGIN      
        1 cgccggcagc acgcagttct cgatcgacct cgggtttccg ttccggtacg cgatcaagcc
       61 cgagatcgcg atcgtcgcgc tgcagacgtt gatgcagatc gacttcaacg aagcgaagac
      121 cgaccatgtc accgtgaagt cggagacgga tccggccacg atgatgacgc ggatcacgtc
      181 ggagaatacg ggcgacggcg cgaagcccga cctcaatccg tcgatcggca tcgcgacgaa
      241 cccggtgccg ccactgtcgg tcgtcgtcta tgcgcagctg cgcattcccg acttcgacac
      301 atcggccggt gcgttccagg tgccggtcac cggccgcatc gagttctcgc cgaaccagaa
      361 gttcgacgtc ggtctcgagt tcacgctgct caacgtcaag ccgcccgacg gacagagccc
      421 gatcgagaat cgtttcctcg cgctcttcgt gcagtcgagg ttcggcaagt gaaagtttgc
      481 ccgcgctgct tcacgcgatt ccccgacggg gagcgcttct gtttgcatga ctccgcggtc
      541 ctcgtcgaag aagaggacat cgcacggctc ggtcacaaca tcggtaacta ccgcctcgac
      601 aagattctcg gtcgcggcgg catgggcacc gtgtacatgg gcgagcacat ctacatcaag
      661 aagcccgttg cggtgaaagt gctgcacccg cagttcgcgc ggtatcagga agcgattcac
      721 cgcttcctgc gcgaggcgcg tgcggcgacg tcgatcaatc acccgaacat cgtcgatgtc
      781 accgacttcg gcatcttgcc cgagggaccg gtctacttcg tgatggagtt cctcgagggc
      841 aagtcgctcg aggatttgat cgagaaggaa ggcgcggtcg agctgcaccg tgcgctgaac
      901 atcgcgaacc agatcgcgct cgctctcgat gcggcgcacg cggtcggcgt gatccaccgc
      961 gacctcaagc ccgacaacat catgctgctg aagcggcccg gtcgccgcga tctagtccgc
     1021 atgactccgg accagaactg gatcaccgag cgcgaggaat cgtacgactt cgtcaaagtc
     1081 ctcgacttcg gcattgcgaa ggtgctgatc caggacgagc tgatggccga gaccgttcag
     1141 ggcgcggtgt tcggaactcc cgagtacatg tcgcccgaag ctgcacgtgg cgaagacgtc
     1201 gaccatcgcg ccgacgtcta ctcgctcggc gtcatcctgt tcgacatgct gtgcggtcgg
     1261 ccgccgttcg aggcgccgca gtcgagcgaa gtgctcgcca tgcacatcca ctcgccgcca
     1321 ccgtcgccgc gcgagttcgc gccgcaccgg gaaatcacgg aacaagccga ggctgtgatc
     1381 ctgaaagcaa tgcaaaagga tccggcgcgc cgctaccaga acatggtcga gttccgtcgc
     1441 gacctcgaga tctcctacgg ttcgatttcg taccgtcggc gcggcgctcc cggcgggcgc
     1501 atcgagccgc gcggcatgga agcgcgcgct cccaagaagc ggctgaccga agagctcgac
     1561 gagtggatca cgaacgaccc atcggggatg tcggtcgagc aagcccgcat gatgtcgctg
     1621 gtcgagacag ccgaaaaggc tttcactcct actggaatgt cgcccgcgga agccgagcgg
     1681 atcgccaacg cactcgatcg cgcgctcgat gacgacgagt agcgtgcaag tttttcgctt
     1741 agcacccccg acgtaacctt ctcggctcgg tagacgtgct agtttgcagc cgttgatgtc
     1801 tgcaaaccaa gccaaccaat caggccaaat cacgttgcgc aaggacgtga aattcaatgg
     1861 cgtgaaggtg ttctcggcga cgatggtggc ggaccgtgat cagttgggcg agaaggtgac
     1921 cgcttggatc caagcgaata aggagaagaa agtcacggac atcgttatca cgcagtcgag
     1981 tgacgaagcc tttcactgca tcgcaatcac cgttttctac tgggaagaca ggacctgagg
     2041 aaactcaatt tctcgtcggg cccagccgtc cttcctgaag ctgtgctgcg gcgagcgcag
     2101 caagcgatct gggatctgga cggatccggc atcggcattc tcgagcacag tcatcgcggc
     2161 aaggagttcg gtcgcgtgct cgatcgcgcc gaagcgctcg tgcgcgaggt gggcggcatc
     2221 ggcgacgaat tcgcgatcct gttcatgacc tgcggtgcga cgcagcactt cgcactcgcg
     2281 ccacagaact ttcttggcgt cggcgtaaca gcggactact gccacaccgg catctggtcc
     2341 ggcaaagcga tggaggatgc gaagcgctac ggcagcgtgc acgtcgcgtg cagcgccgaa
     2401 gcggacaact tcgcaagcgt gccgacaagc ttctcgtggt cggcatcacc acgctacgtg
     2461 cactacacga gcaacgagac gatctacggc acagagtggc agagcccacc gaaggcgccg
     2521 gcaccgctcg tctgcgatgc atcgagcgac atcttcagca agccgctcgt gctcgacggc
     2581 cacgcgatga tctacgccgg tgcacagaag aacctcggtc catcgggcat ctcgctcgtg
     2641 atcgcgcggc gcgacttcat cgcgaccggc acgaaagatc tgccagcgct ctaccagtac
     2701 cgcacgtacg aagcggagcg ctcgatgcac aacacgccca acacgttcgg catctggatg
     2761 atcgcggaag tgtgcgcgtg gatcaaagag caaggcgggc tcgccgcgat ggcggagcac
     2821 aatcgcaaga aggcgcagct gctctacgac gcactcgacg cgagcaaggt ctggcgcacg
     2881 cacgcgaagc cgggcagccg ctcgctgatg aacgtcacgt tccgcggtgc gacgaaagag
     2941 atcgaggaac agctcatcgc gcgcgccgag gagcgcggct tgagtggatt acgtggccac
     3001 cgctcggtgg gagggatgcg cgcgagtctg tacaatgcgt tcccggaaga cggcgtgcgc
     3061 aagctcgtcg atctactgca cgagctcgaa cgatgaaatt cctcacagcg gcggggcagc
     3121 gggcgatcga agacgcggtc gctgcgatcg aggcggtctc ctcggcggag atcgtgatcg
     3181 cggtccggcc gcgtgcgcgc cacgcgctga tgcagcacgc aacggtcggc atcgtcgctg
     3241 cgatcgcgat cctcgcattc acgctctaca gcccgatcga gttctcgctg tggcaaatcc
     3301 tggtgctgcc gatcttcgcc ggcatcctcg gcgcgctgct cgtcgaggcg atcccaccgc
     3361 tgtaccgctt cgtcgagccg ccgtggctgc gctacgagca cgtgcgcgag gccgcgtacg
     3421 cggcattcgt cgagaagcgt gtccacgcga cgcgtgaccg aaccggggtt ctgatctacg
     3481 tcgcggtgcg cgagcggatg gtcgaagtcg tcggcgacct cgcggtggcc gacaagctcg
     3541 gcatcgaggg gatcgctgcg atggcaggca cgctcgaagc gttcgttccc cgcggcagtg
     3601 aagcgttcgg caaggcgctc gccggacagg tcgacgaatt cgcgaaggcg ttgccgcgtc
     3661 gcgccgatga tatcgacgag ctatcgaacg cggtcaccgt gttcggcgct ccgccccgcc
     3721 agcgcaaggc cgtgtcatga gctggccgcc tcgtcgcgcg gcccttctcc tcggtctgtt
     3781 cgtcgtcggt gcactcgccg gcgtcgccgc agcacgcccc ggtggcgggc agagctactc
     3841 cggcggcggt cacagctact cgggtggcgg cggttactcg agcgggcaca gctactcggg
     3901 tggcagctac tcgtcgtcga atagtggtgg tggttcgctc agcgcaggag cggcgctcgg
     3961 aatcctgttc ggcttcgcga tcatgatcgt gatcatcgcg gtggtacagg gtgcgcaatc
     4021 gaacgcgtac acgagtgcgc tcaccgcgcc gacacccccg ccgagactcg atctccagcc
     4081 actactgtcg cgcgatcccg acttctcgcg cgttgcattc gaagacttcg cctaccgatt
     4141 gtactcggag tcacagcgcg cgaggcacgc tcgcgacgag ctcatcaagc tggcgccgta
     4201 tctcggtgcc ggcgccttcg atggcctact gcgcagagca tcgcgtgtcg agcaagtcgt
     4261 gatcggcaac ctgcgtatca tccaggtcgg cgccgagctc gccggtacgc gcacgaaggt
     4321 cgtcgtcgcg atcgaagcga acctcgcggg gccgagtggc acgacgaacg cgttggagcg
     4381 ctggacgttc ctgcgcgcgc cgaacgtgac gacgcgaccg ccggagcgca cgcgaacgtt
     4441 cgcgtgcccg aactgcaatg ctccgttcgc gagcggcgag acgtggcgca cctgcgcaca
     4501 ttgcggcacg gacatcgaaa ccggaaagtt cgattggacg gtcgacgcga tcgccgtcga
     4561 tgccgagacc agcgtcggtc agaccttgac cggcaccgtc gaggaagtcg gcaatgactt
     4621 cccgacgctg atcgagcccg aagcgcaaga acgcctcatg cagctgcgcc aagacgacac
     4681 tgcggtgacc tgggatgcat tcaccgcgcg cgttgcgatg atctacgctc ggctcaacag
     4741 cgcgtggaac gcgcaggagc tcgcgccggt acgcggcctc gtcaccgcct cgctcttgca
     4801 gtacctgcag tactggatcg acgagtacaa gcgccagaca ttcgcgaaca agctcgagga
     4861 agcgcgcatc tcgcacatcg cgctcgcgaa ggtgacgcgc gacaaatggt tcgacgcgat
     4921 cacggtgcgt gtctacgccg acggcctcga cttcacggtc gacaaggagc agcgcgtcgt
     4981 cggcggttcg cgcagcaacc ggcgcgcgta taccgagtac tggacgttca ttcgctcgtc
     5041 ggctcgccgt ggcccagtga ccgtcgatcc gaagtgcccg aactgcggcg cgccgctcgc
     5101 gatcagcaac gcaggtgtat gcacgcattg caac
//
LOCUS       DASUFX010000267         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1429935, whole genome shotgun sequence.
ACCESSION   DASUFX010000267 DASUFX010000000
VERSION     DASUFX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429935"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFV99_10625"
     CDS             <1..856
                     /locus_tag="VFV99_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059805.1"
                     /translation="SQQPKSNPPTSNPPTSQQRPVTPRPAPQASTTSGSFVVPPRRPI
                     GLMVVVLLVDLGLAATGGVLLSKGLASKDEKKSEPKSEPAPVEKKSELETHPAAPAVP
                     AAAVAPAPATSAATAVIAAAPAAEPKPNVAARESPKRDKAATHPTKPDDKPVTKPDDK
                     PITKPDDKPVTKPAEQKGDAEVVAKVPAQPQDPYQAPNTEQEIDAVAARSSAAFARCA
                     AAQSAHGSIKIAFQVRYDGRVINAAAVENTTNNADLARCLVTEISSWHVSAHNGTTIN
                     MLRPFTYP"
     gene            861..1916
                     /locus_tag="VFV99_10630"
     CDS             861..1916
                     /locus_tag="VFV99_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059806.1"
                     /translation="MTIDSGSHRTVHSGAHAALVGVEVLVLDADQRVHAGITQLLAEA
                     SLHVTTATDAERALALIDKSFFAVALVDIDTPTPSAGIATIAEIKHRSPTTMIVALTP
                     RRSYEDAVEAVRAGAVDLILKAPESVAYLKERVLAAAGRSVGKREVDSVLHEVRKVHD
                     EFLERFMAAERRAIDADDKIAGRDHSRMVNIDELRVLVVDEVDDFVSTMEQAKPKGFG
                     FVHASSGGEALDRISSGEFHYAMIAEDVSDLPAKTIAKTIRTQRPDTVVLTFLGPSDN
                     GHVHLIDATGSKPILKPFTDPKQLISRLDELAEAWRAKTKERRYTQAFREKHYDFLRK
                     YVELKTKIDRAIGEVPG"
     gene            complement(1913..>2869)
                     /locus_tag="VFV99_10635"
     CDS             complement(1913..>2869)
                     /locus_tag="VFV99_10635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059807.1"
                     /translation="WMENWLACLPRAEAPKLAILHHDGEPVAACFVGSRWLRRHHVVP
                     SRARFLNATGVEAFDELCLEHNALLSVDPGASLATLVQLLPGNWDEVHLPACDATAFA
                     ALSALGGNLRVRIDREVANYQIDLAKVRDQGYVPLLGASTRAQVRKAQRVAGNVTFEV
                     AGDDRDAITFYNELVALHQRSWRERGQPGAFADPWFDRFHRRLIAQRVRHGEVQLIRV
                     RNEELTIGCLYNFVAADRVLFYQSGFGTPADRHLRPGYLCHAHAVEHCAKQGHAIYDL
                     LGGDARYKQSLATDETRLIWGRVQRKRLQFALEDRARVWLRR"
BASE COUNT          551 a          973 c          922 g          423 t
ORIGIN      
        1 gtcgcagcaa ccgaagagca atccgccgac gtccaacccg ccgacgtcgc agcagcgtcc
       61 ggtgacgccg cgccctgcac cgcaagcatc gaccacatcc ggttcgttcg tcgtgccgcc
      121 gcgccggccg atcggtctca tggtcgtcgt gctgctcgtc gatctcggcc tcgcggcgac
      181 gggcggtgtg ctgctgagca aaggtctcgc gtcgaaggac gagaagaaga gcgaaccgaa
      241 gagcgagccc gcgcccgtcg agaagaagag cgagctcgaa acgcatcctg ccgctccggc
      301 cgtgccggca gctgctgtag caccggcgcc cgccacgagc gccgcgacgg ccgtgatcgc
      361 cgctgcgccc gccgccgaac ccaaaccgaa cgtcgcggcg cgcgaatcgc cgaagcgtga
      421 caaggcagcg acccacccga ccaagcccga cgacaaaccg gtcaccaagc ccgacgacaa
      481 acccatcacc aagcccgacg acaaaccggt gacgaagcct gccgagcaga agggtgatgc
      541 ggaggtcgtc gcgaaggtgc ctgcccagcc gcaggacccg tatcaggctc ccaacaccga
      601 gcaagagatc gatgctgtcg ccgcacggtc gagcgctgcg ttcgcgcgct gcgccgccgc
      661 tcaatccgcg catggctcga tcaagatagc gttccaggtt cgctacgacg gtcgcgtgat
      721 caacgcggcg gccgtcgaga acacgacgaa caatgccgac ctcgcgcgct gtctcgtcac
      781 cgaaatctcg tcgtggcacg tgtccgcgca caacggcacg accatcaaca tgctgagacc
      841 gttcacctac ccgtgatcct atgaccatcg actccggctc tcatcggacc gttcattccg
      901 gcgcgcatgc tgcgctcgtc ggtgtcgaag tgctcgtgct cgacgcagat caacgcgtcc
      961 acgcgggaat cactcagcta ctcgcggaag cgagcttgca cgtcacgacc gcgaccgacg
     1021 ccgaacgtgc gctggcgttg atcgacaaga gcttcttcgc agtcgcactg gtcgatatcg
     1081 acacgccgac gccgagtgca ggcatcgcga caattgccga gatcaagcac cgctcgccga
     1141 cgacgatgat cgtcgcattg acgccgcgcc gctcgtacga ggacgcggtc gaagccgtgc
     1201 gcgctggcgc cgtcgatctc atcctcaagg ctcccgagtc ggtcgcgtat ctcaaggagc
     1261 gcgtgctggc agcggccggt cgttctgtcg gcaaacgcga ggtcgacagc gtgctccacg
     1321 aagtgcgcaa ggtgcacgac gagttcctcg agcggttcat ggcggccgag cgccgtgcga
     1381 tcgacgccga cgacaagatc gccggtcgcg atcactcgcg catggtcaac atcgacgagc
     1441 tccgcgttct cgttgtcgac gaggtcgatg acttcgtgtc gacgatggaa caggcgaaac
     1501 cgaaaggctt tggtttcgtg catgcgtcgt cggggggcga agcactcgac cgcatcagca
     1561 gcggcgaatt ccactatgcg atgatcgcgg aggacgtcag cgaccttccc gcgaagacga
     1621 tcgcgaagac gatccggacg cagcgtcccg acaccgtcgt gctgacgttc ctcggtccgt
     1681 cggacaatgg ccacgtccat ctcatcgacg cgacgggaag caagccgatc ttgaagccgt
     1741 tcaccgatcc gaagcagttg atcagccggc tcgacgagct tgccgaagcg tggcgcgcga
     1801 agacgaagga acgccgctac acgcaggcgt tccgcgagaa gcactacgac ttcttgcgca
     1861 agtacgtcga gctgaagacg aagatcgatc gcgcgatcgg cgaagttccg ggctaacgcc
     1921 ggagccacac gcgcgcgcga tcttcgagcg cgaactgcaa gcgcttgcgc tgcacgcgtc
     1981 cccagatcag ccgcgtctcg tcggtcgcga ggctctgctt gtagcgggcg tcgccgccga
     2041 gcaagtcgta gatcgcgtgg ccctgcttcg cgcaatgctc gaccgcgtgg gcatgacaga
     2101 ggtagccggg gcgcaggtga cgatcggccg gcgtgccgaa gcccgactga tagaacagca
     2161 cgcgatcggc ggcgacgaag ttatagaggc agccgatcgt cagctcttcg ttccgcacgc
     2221 ggatgagctg cacttcgccg tggcggacgc gctgcgcgat cagccggcga tgaaagcgat
     2281 cgaaccacgg gtcggcgaac gcaccgggct ggccgcgctc gcgccacgat cgctgatgga
     2341 gcgcgacgag ctcgttgtag aacgtgatcg cgtcgcgatc gtcgcccgca acctcgaacg
     2401 tcacgttgcc ggccacgcgt tgcgccttgc ggacctgcgc gcgcgtcgac gcgccgagca
     2461 gcgggacgta gccctggtcg cggaccttcg cgagatcgat ctgataattc gcgacttcgc
     2521 gatcgatccg cacgcgcagg ttgccgccga gcgcgctgag cgcagcgaac gcggtcgcat
     2581 cgcatgccgg cagatgaacc tcgtcccagt tgcccggcag cagctgcacg agcgtcgcga
     2641 gcgacgcgcc aggatcgacc gacaacagcg cgttgtgctc gaggcagagc tcgtcgaacg
     2701 cctcgacgcc agtcgcgttg agaaaccgag cgcggctcgg caccacgtga tgacggcgga
     2761 gccatcgact cccgacgaaa caggcggcca ccggctcgcc gtcgtgatga aggatcgcga
     2821 gcttgggcgc ctcggcgcgc ggcaagcacg cgagccagtt ctccatcca
//
LOCUS       DASUFX010000268         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_41963,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000268 DASUFX010000000
VERSION     DASUFX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41963"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..536)
                     /locus_tag="VFV99_10640"
     CDS             complement(51..536)
                     /locus_tag="VFV99_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX5059808.1"
                     /translation="MTGVVAWFTGLPSSGKTTLAGAVGNELKARGIKPVMLDSDDLRA
                     SITPPLGYDNGSRAALYGTLARIAALVANQGHIVLVPATAHRRVFRDAARSLAPQFVE
                     IYVDTPLEECKRRDRKGTYAGAGELVPGVGVDYEPPVNPDFRAADTAARIAQRLADLA
                     R"
     gene            complement(565..1620)
                     /locus_tag="VFV99_10645"
     CDS             complement(565..1620)
                     /locus_tag="VFV99_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5059809.1"
                     /translation="MLPTGLRARLEQLFPGATIDHVTALRDDESSGEAMKALGYGKPL
                     RIELTVAGARQSLVFHTARADDFGHDRRSDRVGNLVLAFDTFGKLPNHVRAIDAGVVR
                     DDGALVSIAGTGEPYLLTTWVDGMLYADELRRIAKAGKALSQDLVHAERLATLLLEIH
                     SVHGSHAGAYTRAIRDLLGHGEGIFGLVDSYAPSDLTPRLRRLEEACLRWRWKLRDRT
                     ERLRRTHGDCHPFNIIVADDVYLLDASRGSEGDPADDVACLTINFLFMGVGHPRWAGG
                     LGTLWERFWQTYLAGDRVDVLAAVAPFYAWRALVLASPKWYPHTADADRDRILTFAER
                     ALAAERFDPAWGAEAMA"
     gene            complement(1620..2582)
                     /locus_tag="VFV99_10650"
     CDS             complement(1620..2582)
                     /locus_tag="VFV99_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059810.1"
                     /translation="MDLGRMLEKCVRDQWSIDDLDWSLPPPTLDRDKEEAVVQAFIDM
                     SGIELLAGALFEVQRDKTKDPTLKKIFSTFVADEKRHSAVAARLAKHYDVHHYREYKE
                     SESLTKFRPHFLALARKASPEIANAYITSGELILDVALLRSLDDYVADEMSHRAMHLI
                     NRDESRHIAIDFHMTEHYSSDEYLAHLRTRRRPSMRELASGLRALVTMMWHARPFLQE
                     VFLAPMDRTDPSGRRVQEAFKRIQLVMRKPTVARQPFSKFMIGTQNLYNHPIWGRLFG
                     PLFLRALGAEDRAARFLFTREELERSQRMSFEELAEEAVAAKYS"
     gene            2802..4265
                     /locus_tag="VFV99_10655"
     CDS             2802..4265
                     /locus_tag="VFV99_10655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059811.1"
                     /translation="MKRLAAGGMADVLLARTDGIAGFERHVVIKRIHAELLEEPRYVK
                     MFLDEARLAASLHHHNVVQVNDIGEENGEYFFAMEYVHGEDARQLLVDASQRGEQIPL
                     EHVVTIVMAAASGLHYAHEQRSMDGKPLHIVHRDVSPANILVGYDGGVKVADFGIALA
                     AHRAEQTQSGVLKGKVAYMSPEQCNCDPVDRRSDVFSLGIVLYELVTVRPCFAGDNDF
                     MTMSAIVGGKIEKPSSFRKDLPFELEHIIMKALAKNPADRYQSADELRMALEAFATAA
                     QLRTSMTALADYMKKQFGAKALPWQEDDGEPEMTFAVPHEDIDTPAPEETDDDDDLSI
                     PLAAPEEGKVRAPTLPMRALDNRPPAQIVVHTTPSSKRIDRRRMISEGKAAQARKSRK
                     LWLAGSAIAVLIAAMSLIIVSTHSSNKTVPRASITKPETVHPRSSVPEPMTPSTVKSQ
                     AAAPAQTPAAAPEKKSRRGKTAPKPAKKWDPDALFPQ"
     gene            4284..>4947
                     /locus_tag="VFV99_10660"
     CDS             4284..>4947
                     /locus_tag="VFV99_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5059812.1"
                     /translation="MQRALYALFILGALAAACGSDEHSATLKAIRDRGYLTWGADLQG
                     GEPYLYEDPANPKNIVGFEVDIAHAIARRMGVDAHFVQYNWSNLVPSLERGDFDIALN
                     GLEYTSERAERILLSQPYFTYAETLAVRVDSPYKSLADLEGKRVGTLNQTYAHDLLRA
                     QPLEPVLYEGNEEPYIDLEQGRVDAVLLDNIIADRYGCVPSRPTIKCLPDDVARGTYV
                     IGI"
BASE COUNT          904 a         1591 c         1623 g          829 t
ORIGIN      
        1 agtgagctgg ttggatgtcg tcgatcttca tgccgcgacg ctaacgcaat tcagcgcgcg
       61 agatcggcga ggcgctgcgc gatccgtgcg gcggtgtcag cggccctgaa gtccggattg
      121 accggcggct cgtagtcgac accgacgccg ggcacgagct cgcccgcgcc ggcgtacgtc
      181 cccttgcgat cgcggcgctt gcactcttcg agcggcgtat cgacgtagat ctcgacgaac
      241 tgtggcgcca ggctgcgcgc agcatcgcga aacacgcggc gatgcgcggt cgccggcacg
      301 agcacgatgt ggccttgatt cgcgacgagc gcggcgatcc gggcgagcgt gccgtagagc
      361 gccgcacgcg agccgttgtc gtaaccgagc ggcggcgtga tcgacgcgcg caggtcgtcg
      421 ctgtcgagca tgaccggctt gatgccgcgc gccttcagct cgttaccgac cgcgcctgcg
      481 agcgtcgtct tgcccgacga cggaagaccc gtgaaccacg cgaccactcc ggtcatgcca
      541 ttgcctccgc tcctccgctc tccatcatgc cattgcttcg gcaccccagg cgggatcgaa
      601 ccgctcggcg gcgagcgcgc gctcggcgaa cgtcaggatg cgatcgcgat ccgcatctgc
      661 cgtgtgcggg taccacttcg ggctcgcgag cacgagcgcg cgccaggcgt agaacggcgc
      721 gacggcagcg agcacgtcga cgcgatcccc cgcgaggtac gtctgccaga atcgctccca
      781 cagcgtgccg agcccaccgg cccagcgtgg gtgaccgaca cccatgaaca ggaaattgat
      841 cgtcaggcac gcgacgtcgt cggccggatc gccttcgctg ccgcgactcg cgtcgagcaa
      901 gtagacgtcg tcggcaacga tgatgttgaa cggatggcag tcgccgtgcg tgcggcggag
      961 ccgttcggtg cggtcgcgaa gcttccagcg ccagcgcagg cacgcctctt cgagccggcg
     1021 caatcgcggc gtcaggtcgc tcggtgcgta gctgtcgacg aggccgaaga tgccttcgcc
     1081 gtggccgagc agatcgcgga tcgcgcgcgt gtacgcgccg gcatgcgagc catgcacgct
     1141 gtggatctcg agcagtaatg ttgcgagccg ctcggcgtgc accaggtcct gcgacaacgc
     1201 tttgccggcc ttcgcgatgc gacgcagctc gtctgcgtac agcatgccgt cgacccacgt
     1261 cgtcaagaga tacggctcgc cggtgccggc gatcgagacg agcgcgccat cgtcacgcac
     1321 gacgcctgca tcgatcgcgc ggacgtgatt cggcagcttg ccgaacgtgt cgaacgcgag
     1381 cacgagattg ccgacgcgat ccgagcggcg atcgtggccg aagtcatcgg cgcgtgcggt
     1441 gtggaacacg agtgactggc gcgcgccggc gaccgtgagc tcgatgcgca gcggcttgcc
     1501 gtagccgagc gccttcatcg cttcgccgct cgactcgtcg tcgcgtagcg cggtgacatg
     1561 gtcgatcgtc gcccctggga acagctgctc gaggcgagcg cgcagtccgg ttggcagcat
     1621 caggaatact tagcagcgac cgcttcttcg gcgagctctt cgaagctcat ccgctggctt
     1681 ctctctagct cctcacgcgt gaacaagaac cgcgctgcgc gatcttccgc accgagtgcg
     1741 cgcaagaaca gcgggccgaa caaccgtccc cagatcggat gattgtagag gttctgcgtg
     1801 ccgatcatga atttcgagaa tggttggcgc gcaaccgtcg gcttacgcat cacgagctgg
     1861 atacgtttga atgcttcctg aacgcggcga ccggacggat cggtgcgatc catcggcgcg
     1921 aggaagacct cctgaaggaa cgggcgcgca tgccacatca tcgtgacgag cgcgcgaagg
     1981 cccgacgcga gctcgcgcat gctcggccgg cggcgcgtgc gcaggtgcgc gaggtactcg
     2041 tcagacgagt agtgctcggt catgtggaag tcgatcgcga tgtggcgtga ctcgtcgcgg
     2101 ttgatgaggt gcatcgcgcg atggctcatc tcgtccgcga cgtaatcgtc gagggaccgt
     2161 agcaacgcca cgtcgaggat gagctcacct gaggtgatgt aggcgtttgc gatctcgggt
     2221 gaggctttgc gcgccaatgc gaggaagtgc ggtcggaact tcgtcagcga ctcgctctcc
     2281 ttgtactcgc gatagtgatg cacgtcgtag tgcttcgcga gacgcgcagc aactgcgctg
     2341 tgacgtttct cgtcggcgac gaacgtcgag aagatcttct tgagagtcgg atctttcgtt
     2401 ttgtctcgct ggacttcgaa gagcgcgccg gcgagcagct cgatgccgga catatcgatg
     2461 aaggcctgca cgacggcctc ttctttgtcg cggtccaatg taggtggcgg tagcgaccag
     2521 tcgaggtcgt cgatcgacca ctggtctcgg acacacttct ccagcatgcg ccccaggtcc
     2581 atgatcgaac tctaacggat tgcgcgtcgc gtttcttcgc cgctcgacgt cagtactggc
     2641 aatgagaatc tcgtagtgct tggtacacaa agtttcgcgc tgacagttcc tacattcagc
     2701 aatgcttttc agtacttgat cgattcgcga gaaggcatca atttgaaaga gtgaacattc
     2761 gcgcggccac ttccaccacg catctcggca ggtatcacgt ggtgaagcgg ctcgcagcag
     2821 gcggcatggc cgacgtgttg ctcgcacgaa ccgacggcat tgctggattc gaacgccacg
     2881 tcgtcatcaa gcgcattcac gccgagctgc tcgaagagcc gcgctacgtg aagatgtttc
     2941 tcgacgaggc gcgcctcgct gcgtcgctgc atcaccacaa cgttgtccag gtcaacgaca
     3001 tcggcgagga gaacggcgag tacttcttcg cgatggaata cgtgcacggc gaagacgcgc
     3061 gccagctgct cgtcgatgcg agtcagcgtg gcgagcaaat tccgctcgag cacgtcgtca
     3121 cgatcgtcat ggccgccgcg tcgggccttc actacgcgca cgagcagcgc agcatggatg
     3181 gcaagccgct gcacatcgtc catcgcgacg tgtcgccggc gaacatcctt gtcggttacg
     3241 acggcggtgt gaaggttgcc gacttcggca tcgcgctcgc tgcgcatcgt gccgagcaaa
     3301 cgcagtcggg cgtgctcaag ggcaaggtcg cgtatatgtc gcccgagcag tgcaactgcg
     3361 atccggtcga ccgcaggagc gacgtgttct cgctcggcat cgttctctac gagctcgtca
     3421 cggtgcggcc gtgcttcgcg ggcgataacg acttcatgac gatgtcggcg atcgtcggcg
     3481 gcaagatcga gaagccgagc agcttccgca aggatctgcc gttcgagctc gagcacatca
     3541 tcatgaaggc gctcgcgaag aacccggcgg atcgctacca gagcgccgac gagctgcgca
     3601 tggcgctcga ggcgttcgcg accgcggcgc agctgcggac gtcgatgacg gcgctcgccg
     3661 actacatgaa gaagcagttc ggtgcgaagg cgctgccgtg gcaggaagac gacggcgagc
     3721 ccgagatgac gttcgcggtg ccgcacgagg acatcgacac gccggctccc gaagagaccg
     3781 acgacgacga cgacctgtcg attccgttgg ccgccccgga ggagggcaaa gtgcgcgcgc
     3841 caacgctgcc gatgcgcgcg ctcgacaacc ggccaccggc gcagatcgtg gtccacacca
     3901 cgccatcatc gaagcggatc gatcgccgcc gcatgatcag cgagggcaag gccgcacagg
     3961 ctcgcaagag ccgcaagctg tggctcgccg gctcggcgat cgcagtgctg atcgcggcga
     4021 tgtcgctgat catcgtcagc acacacagca gcaacaagac cgtgccgcgc gcgagcatca
     4081 cgaaaccgga gaccgtccat ccgcgttcga gcgtgccgga accgatgaca ccgtcgacgg
     4141 tgaagagcca ggccgcagcg ccggcccaga caccggcagc ggcgcccgag aaaaagtcgc
     4201 gtcgaggaaa aacggcgccg aagcccgcga agaagtggga tccggacgcg ttgttcccgc
     4261 agtaactcgc gcatactcgg cgcgtgcaac gcgcgctcta cgcgctcttc atcctcgggg
     4321 ctctcgcggc cgcgtgcggc agcgacgagc acagcgccac gctcaaggcg atcagggacc
     4381 gcggctacct gacgtggggc gcggacctgc agggcggcga gccgtacctt tacgaggatc
     4441 cggcgaatcc gaagaacatc gtcggcttcg aggtcgatat cgctcacgcg atcgcgcgcc
     4501 gcatgggcgt cgatgcgcac ttcgtccagt acaactggtc gaacctggtg ccatcactcg
     4561 agcgcggcga tttcgacatc gcgctcaacg gactcgagta cacgagcgaa cgcgccgagc
     4621 ggatcctgct gagccagccg tacttcacgt acgccgagac gctcgcagtg cgcgtcgaca
     4681 gcccgtacaa gtcgctcgcc gacctcgagg gcaagcgcgt cggcacgctg aaccagacct
     4741 acgcacacga cctgttgcgc gcgcagccgc tcgagcccgt gctctacgag ggcaacgagg
     4801 agccgtacat cgatctcgag cagggtcgcg tcgacgcggt gctgctcgac aacatcatcg
     4861 ccgaccgtta cggctgcgtg ccgagccggc cgacgatcaa gtgcctgccc gacgatgtcg
     4921 cgcgtggcac gtacgtgatc ggcatcc
//
LOCUS       DASUFX010000269         6255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1430483, whole genome shotgun sequence.
ACCESSION   DASUFX010000269 DASUFX010000000
VERSION     DASUFX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430483"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFV99_10665"
     CDS             <1..747
                     /locus_tag="VFV99_10665"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5059813.1"
                     /translation="GLRFLLALIIVVAGRQIARFILRTLDRFMERNNMDVSLRKFVGD
                     LAYAVLLVSVIIAALDTLGVKTTAVIAVLGAAGLAIGLALQGSLSNFAAGVMLIVLRP
                     YKVGDLVMIDKYLGRVEAIKVFNTVVLTGDHREVTIPNSLIIAKPIENLTALGRRRVD
                     LVVSVAQSSDLHRVRQLLEGVVLADTRIQQAPSPTIDIAEITDASVKLHLRPWTSVEN
                     YTKVATDTMERIKQTMDAAELKYSVALVPA"
     gene            744..1097
                     /locus_tag="VFV99_10670"
     CDS             744..1097
                     /locus_tag="VFV99_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX5059814.1"
                     /translation="MNKALVAAGAINAGLAVAAGAFAAHGLRERLEARALEIFETGAR
                     YQMYHALAMILCAVIATSGAKTAGWILQAGIVVFSGSLYALALTDVKILGAITPLGGF
                     AFLVGWAWLAITALR"
     gene            complement(1094..2104)
                     /gene="rtcA"
                     /locus_tag="VFV99_10675"
     CDS             complement(1094..2104)
                     /gene="rtcA"
                     /locus_tag="VFV99_10675"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX5059815.1"
                     /translation="MIEIDGSRGEGGGQILRTSLALSMITKKPIRMRRIRAGRKKPGL
                     QRQHLACVEAAAQLCNAHVRGADVSSQELEFTPGEGCAEHLTIDIGTAGSTTLVVQTI
                     LVPAIALGHPLNATVIGGTHNPMAPPFEFLDRVFLPHLRAMGAHVTLKLDKHGVFPAG
                     GGRVTLEVRPASKLKPIAVVEAGPVTGKRASVIIARLPTHVADRELAIVRERFKIAPE
                     DCETVDLRGGPANILMIEIERAGSREIVSGHGEKGLRAELVAERACDEMARYLDADVP
                     VGVHLADQLLLPMALAHGGRFRAQGPLSLHATTNIETIQMFVDVPIRVEQSDEIADVI
                     VG"
     gene            complement(2106..3320)
                     /locus_tag="VFV99_10680"
     CDS             complement(2106..3320)
                     /locus_tag="VFV99_10680"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5059816.1"
                     /translation="MSNYELIETVGVPIKAWTRGVGVEDGARKQLENVARLPIVFHHV
                     AVMPDVHFGIGATVGSVVPTEKAIIPAAVGVDIGCGMSAVETDLDAKLLPDSLKSLRH
                     AIEKAVPHGRTANGGRGDRGAWHNVPKHTASAWKSLEDDYKTIIAKHPRVGQGNDINH
                     LGTLGGGNHFVEVCLDETDKVWFMLHSGSRGVGNRIGTYFIALAKEDMRRHVKNLPDS
                     DLAYLREGTDHFNDYALAVSWGQRFALTNRQLMMEAVLNAARKELPPFQLGEYAVNCH
                     HNYVEREHHFGKDVFVTRKGAVRARLGDLGIIPGSMGAKSFIVRGKGNPDSFMSCSHG
                     AGRVMSRGEAKRRFTVEDHEKATAGIECKKDASVIDETPMAYKDIDKVMEAQSDLVEV
                     VHQLRQVVCVKG"
     gene            3466..3828
                     /locus_tag="VFV99_10685"
     CDS             3466..3828
                     /locus_tag="VFV99_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059817.1"
                     /translation="MWFLAHRKGSTKVVPDGQTFTEECPTCQKRTRFVEVAITAKYGL
                     FFVVDMIKDEERAFACTKCGDIFDLKDTDAPATKPTPSKPTPSKPIVKPEVDREAVAK
                     IERKIDDELAELKRRMGK"
     gene            3872..4783
                     /locus_tag="VFV99_10690"
     CDS             3872..4783
                     /locus_tag="VFV99_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059818.1"
                     /translation="MGRLVLTLVVCGCSFDHGKSLDDAQLPDDDMMVIDTEIDGPATF
                     GPWSPPMKFPELTDTGTVLDDPTLTDDLLEIYFDTDRLGDDDIFFAKRAKTTDPWGPP
                     TLVANINTTSRESDPELSPDGLTLYFSSDRGGGMGGDDIYMTKRTSRTAAWMTETRVT
                     SLNSAGSDDAPTMDGTKQRVVFFSTRTGGAGGGDLYEAKANGATWTTPARITELNTAS
                     NDYDPFLTSSGLGMFFDSDRTGGMGQQDLYETYRPSLTDPFVSPVLLTELNSSGRDMD
                     PWLTTDLRTMVFASDRSGEFEIYITAR"
     gene            complement(4784..5212)
                     /locus_tag="VFV99_10695"
     CDS             complement(4784..5212)
                     /locus_tag="VFV99_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059819.1"
                     /translation="MKPLLLALLLAACGCPQKTDNGTPPPATNPATNPTPTTPSTPAT
                     PPATDPTPPPTQGSGSPSGSGASEPTPNWPKAAPGIGENCGDKDACAKGLTCVAYYGI
                     AGARGPQFKTCEIKCTADANCPADHKCITIADGPGQVCRK"
     gene            complement(5209..>6255)
                     /locus_tag="VFV99_10700"
     CDS             complement(5209..>6255)
                     /locus_tag="VFV99_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5059820.1"
                     /translation="RTYRIRLKQGFIFSGIFPLLFAIAGLGTTALVWVGGNQVIDGNI
                     SAGDWYLFLQAVALFWFPLTSIASFWSQFQQGLSAAERVFALVDAEALVVQTDDIAPA
                     RLSGKIEFRDVTFGYKDERPVLENFSLTIAPGETIALVGHTGAGKSTLGRLISRFYEF
                     QGGQILIDDQDIRSFDLGAYRRQLGIVPQLPFLFSGTVADNIRYALPDASHEQIDFAA
                     SHIGQGDWVDALQDGLDTEIGEEGRGLSMGQRQLVALARVLIQDPAIIILDEATASVD
                     PLTEAQIQEGLATVMAGRTSIVIAHRLSTIREVDRIIVLDHGKIIERGAHPQLLAQGG
                     RYATLFNLQAQGYR"
BASE COUNT         1080 a         2064 c         1991 g         1120 t
ORIGIN      
        1 gggctgcgat ttctcctcgc actgatcatc gtcgtcgccg gccgccagat cgcgcggttc
       61 attctgcgca cgctcgatcg gttcatggag cgcaacaaca tggacgtgtc gctgcgcaag
      121 ttcgtcggcg acctcgcgta cgcggtgctg ctcgtctcgg tgatcatcgc ggcgctcgac
      181 acactcggcg tcaagacaac cgcggtcatc gcggtgctcg gcgctgcagg cctcgcgatc
      241 ggtctcgcat tacaaggctc gctatcgaac ttcgcggcag gcgtgatgct gatcgtgctg
      301 cggccgtaca aggtcgggga cctcgtgatg atcgacaagt acctgggccg cgtcgaggcg
      361 atcaaggtgt tcaacaccgt cgtgctcacc ggtgaccatc gcgaggtgac gattccgaac
      421 agcttgatca tcgcgaagcc gatcgaaaac ctgacggcgc tcggccgccg ccgtgtcgac
      481 ctcgtcgtca gtgttgcgca gtcgtcggat ctgcaccgcg tgcgccagct gctcgagggc
      541 gtcgtgctcg ccgacacgcg gatccagcag gcgccgtcgc caacgatcga tatcgccgag
      601 atcaccgacg cgagcgtgaa actccacctg cggccgtgga cctcggtcga gaactacacg
      661 aaggtcgcca ccgatacgat ggagcgaatc aaacaaacga tggacgcggc ggagctcaag
      721 tactccgtcg cgctggtgcc ggcatgaaca aggcacttgt cgccgccggc gcgatcaacg
      781 cgggccttgc cgtcgctgcc ggtgcgttcg cggcccacgg cttgcgcgag cggctcgaag
      841 cacgcgccct cgagatcttc gagaccggcg cgcgctatca gatgtatcac gcgctcgcga
      901 tgatcctgtg cgcggtgatc gcgacgagcg gtgcgaagac cgcgggctgg atccttcagg
      961 ctggtatcgt cgtgttctcg ggcagcctgt atgcgctcgc gctcaccgac gtgaagatcc
     1021 tcggcgcgat cacgccgctc ggaggtttcg cgttcctcgt cggctgggcg tggctcgcga
     1081 tcacggcgct gcgttagccg acgatcacgt ccgcgatttc gtccgactgc tcgacgcgaa
     1141 tcggcacgtc gacaaacatc tggatcgtct cgatgttcgt cgtcgcatgc agcgacagcg
     1201 ggccttgcgc acgaaatcgt ccgccgtgcg ccaacgccat cggcagcagg agttgatcgg
     1261 ccaagtgcac gccgaccggc acatccgcat cgagataacg cgccatctcg tcgcaggcgc
     1321 gttctgcgac cagctccgca cggaggcctt tctcgccgtg accgctcacg atctcgcgcg
     1381 acccagcgcg ctcgatctcg atcatcagga tgtttgccgg cccgccacgc agatcgacgg
     1441 tctcgcaatc ctcaggagcg atcttgaacc gctcgcgcac gatcgcgagc tcgcgatcgg
     1501 caacgtgcgt tggcagccgc gcgatgatca cgctcgcgcg cttgccggtg accggcccgg
     1561 cctcgacgac cgcgatcggt ttcagcttgc tcgccggtcg cacctcgagc gtgacgcgcc
     1621 cgccgcccgc gggaaacacg ccgtgcttgt cgagcttgag cgtgacgtgt gcacccatcg
     1681 cgcgcaggtg cggcagaaat acgcgatcga gaaactcgaa cggcggcgcc atcgggttgt
     1741 gcgtgccgcc gatcaccgtc gcgttcaacg gatgaccgag cgcgatcgcg ggcaccaaga
     1801 tcgtctgcac gacgagcgtc gtcgagccgg cggtaccgat gtcgatcgtc aggtgctcgg
     1861 cgcagccttc gcccggcgtg aactcgagct cctgcgagct gacgtctgcg ccacgcacgt
     1921 gcgcgttgca cagctgcgcc gcggcttcga cgcacgcgag atgctggcgc tgcaatcccg
     1981 gcttcttgcg gcccgcgcga atccgccgca tgcggatcgg cttcttcgtg atcatcgaca
     2041 gcgcgagcga cgtgcgcagg atctgcccac caccttcgcc gcgcgagccg tcgatctcga
     2101 tcacgttagc ccttcacgca aacgacctgg cgaagctgat ggacgacctc gacgaggtct
     2161 gactgtgctt ccatcacctt gtcgatatct ttgtacgcca tcggcgtctc gtcgatgacg
     2221 ctcgcgtcct tcttgcactc gattccggcg gtcgccttct cgtgatcctc gaccgtgaac
     2281 cggcgcttcg cctcgccgcg tgacatcacg cgtcccgcac cgtggctgca cgacatgaac
     2341 gaatccgggt tgcccttgcc gcgcacgatg aagctcttcg cacccatcga gcccggaatg
     2401 attccgagat cgccgagccg cgcgcgcacc gcacccttac gcgtcacgaa gacatctttg
     2461 ccgaaatggt gctcgcgttc gacgtaattg tgatggcagt tgaccgcgta ctcgccgagc
     2521 tggaacgggg gcagttcttt ccgcgccgcg ttcagtaccg cttccatcat cagctggcga
     2581 ttcgtcagcg cgaaccgctg accccagcta actgcaaggg cgtaatcgtt gaagtggtcg
     2641 gtgccttcgc gcagatacgc gaggtcacta tccggcaggt tcttcacgtg ccggcgcatg
     2701 tcttccttcg cgagcgcgat gaagtaagta ccaatccggt tgccgacgcc gcgcgagccg
     2761 gagtgcagca tgaaccagac cttgtcggtc tcgtcgaggc acacctcgac gaagtggtta
     2821 ccgccgccga gcgtgccgag gtggttgatg tcgttgccct ggccgacgcg cgggtgcttc
     2881 gcgatgatcg tcttgtagtc gtcctcgagg ctcttccacg ccgacgccgt gtgcttcggc
     2941 acgttgtgcc acgcaccgcg atcgccgcgg ccgccgtttg ccgtgcgtcc gtgcgggacg
     3001 gccttctcga tcgcgtgacg cagcgacttg agcgaatcgg gcagcagctt cgcgtcgaga
     3061 tcggtctcga ccgcggacat tccacagcca atatccaccc cgacagccgc cggaatgatc
     3121 gccttttccg tcggcacgac gctgccgacc gttgcaccga taccgaagtg cacgtcgggc
     3181 atcaccgcga cgtgatgaaa cacgatcggc aaccgcgcga cgttctccag ctgcttgcgc
     3241 gcgccgtcct cgacgccgac gccacgcgtc cacgccttga tcggcacgcc gaccgtctcg
     3301 atgagctcgt aattcgacat ggctctacac agggctgcct tcccacagcc tacgtcgcaa
     3361 acaaacctcc ggctcgcgac gcgaggccct tacttacgcg acctctcaaa cggctgcaat
     3421 acgaatgttg gaagcggccg aatcgagccg tgttatgtgc ggtgcatgtg gtttctcgcg
     3481 catcgcaagg gctccacgaa ggtcgtgccg gacgggcaga cgttcacaga ggagtgcccg
     3541 acgtgtcaga aacgcacccg cttcgtcgaa gtcgcgatca ccgcgaagta cggcctgttc
     3601 ttcgtcgtcg acatgatcaa ggacgaggaa cgcgcgtttg cctgcaccaa gtgcggcgac
     3661 atcttcgatc tgaaggacac cgacgcaccc gcgacgaagc cgacgccgtc gaagccgacg
     3721 ccgtcgaagc cgatcgtcaa gccggaagtc gaccgcgagg cggtcgcgaa gatcgagcgc
     3781 aagatcgacg acgagctggc cgagctcaag cgccgcatgg gcaagtagcg ttcgcttcct
     3841 gcggaccgac gacttgtggc accgtagaag ggtgggccgt ctcgtcctca cgctcgtcgt
     3901 ctgcggctgc agcttcgacc acggcaagtc gctcgatgat gcgcagctac cggacgacga
     3961 catgatggtg atcgacacgg agatcgacgg ccccgccaca ttcgggccgt ggagtccgcc
     4021 tatgaagttc cccgagctca ccgataccgg tacggtgctc gacgatccga cgctgacgga
     4081 cgatctgctc gagatctact tcgacaccga tcggctcggc gacgatgaca tcttctttgc
     4141 caagcgagcg aagaccacag atccgtgggg gccgccgacg ctggtcgcga acatcaacac
     4201 cacgtcacgc gagagcgacc cggagctttc gccggacggc ctgacgctct acttctcgag
     4261 cgatcgcggc ggcggcatgg gcggcgacga catctacatg acgaaacgca cgtcgcgcac
     4321 ggcggcgtgg atgaccgaga cgcgggtgac gtccctgaat tcggccggca gcgatgatgc
     4381 gccgacgatg gacggcacga agcagcgcgt cgtgttcttc tcgacgcgca ccggcggcgc
     4441 gggcggtggc gatctctacg aggcgaaggc gaacggtgcc acgtggacca cgccggcccg
     4501 catcaccgag ctgaatacgg cgtcgaacga ctacgatccg ttcttgacgt cgagcggcct
     4561 cggcatgttc ttcgactccg atcgcaccgg cggcatgggg caacaagatc tgtacgagac
     4621 atacaggccg tcgctcaccg atccgttcgt gtcaccggta ttgctcacgg agctcaattc
     4681 gtccgggcgt gacatggacc cgtggctgac gaccgatctg cgcacgatgg tgttcgcgag
     4741 tgatcgcagc ggcgagttcg agatttacat caccgcgcgc tgactacttg cggcagacct
     4801 gaccgggacc gtcggcgatc gtgatgcact tgtgatcggc ggggcagttc gcgtctgccg
     4861 tgcacttgat ctcgcaggtc ttgaactgcg gaccgcgcgc gccggcgatg ccgtagtacg
     4921 cgacgcaggt gaggcctttc gcgcacgcgt ccttgtcgcc gcagttctcg ccgatgccag
     4981 gcgctgcctt cggccagttc ggcgtcggct cgcttgctcc ggagccagac ggcgagcccg
     5041 agccctgcgt cggtggtggt gtcgggtctg tcgccggtgg cgtcgcaggc gtcgagggcg
     5101 tcgtcggcgt cggattcgtg gccgggttcg tcgccggcgg gggcgtgccg ttatcggtct
     5161 tctgcggaca accacacgcg gcgaggagca gagcgagcag gagcggtttc atcggtaccc
     5221 ttgggcttgc agattgaaca gcgtcgcgta gcggccgcct tgagcgagca gctgggggtg
     5281 cgcgccgcgt tcgatgatct tgccatgatc gagcacgatg atccggtcga cttcccggat
     5341 cgtgctcaac cgatgcgcaa tcacaatcga tgttctgccg gccatgacgg tcgccagtcc
     5401 ctcctggatc tgcgcttcgg tgagcggatc gacgctcgcg gtcgcctcat cgaggatgat
     5461 gatcgccgga tcctggatca acacccgcgc cagcgcgacg agttgccgct ggcccatcga
     5521 cagtccacgc ccctcctcgc cgatctcggt gtcgaggccg tcttgcaagg cgtcgaccca
     5581 gtcgccctgg ccgatgtgcg aggcggcgaa atcgatctgc tcgtgcgacg cgtcgggcag
     5641 cgcgtagcgg atgttgtcgg ctacggtccc cgagaagagg aatgggagct gcggaacgat
     5701 cccgagctgt cgccggtacg cgccgagatc gaatgaccgg atgtcctggt cgtcgatcag
     5761 gatttgccca ccctggaact cgtagaagcg cgagatcaac cggccgagcg tcgacttgcc
     5821 ggcgccggtg tggccgacca gggcgatcgt ctcgcccggc gcgatcgtca gcgagaagtt
     5881 ctcgaggacc ggtcgctcat ccttgtagcc gaacgtgaca tcgcggaact cgatcttgcc
     5941 ggaaagacgc gcgggcgcga tgtcgtctgt ctgtaccacc agcgcctcgg catcgactaa
     6001 tgcaaagacc cgctcggccg ccgagagccc ctgctggaac tgactccaga acgacgcaat
     6061 gctcgtcaac gggaaccaga agagggcgac cgcctgcagg aagaggtacc agtcgccggc
     6121 cgagatattg ccgtcgatca cctggttgcc gccgacccag accaacgcag tcgtccccaa
     6181 cccggcgatc gcgaagagga gcgggaagat accgctgaag atgaagccct gcttgagacg
     6241 gatccggtag gttcg
//
LOCUS       DASUFX010000270         6981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_181645,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000270 DASUFX010000000
VERSION     DASUFX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181645"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VFV99_10705"
     CDS             complement(<1..1000)
                     /locus_tag="VFV99_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059821.1"
                     /translation="MRVAVLALLVGCSFEHGVALDDATGGDIATPDGDGRCGLFLDLC
                     GSPAPPGPLHLVNADTINTDTDPRCLGVMQASGGDVCLVYATMVDIDSGASLTATGTR
                     PLAIAASGALTVGGTIDVSSRISGQRGPASSDTACSFAATPELDLGGAGGGAGGSFGL
                     AGGNGGTGDTDQSQGGSDGMGAPGMAGAPASVTALRGGCRGQAGANEDATGGVGGSGG
                     HSGGALYLYAAESIQISGVIRATGAGGNGGQVQAGGGGGGSGGLVVIESPSIVVTGAI
                     SANGGGGGEGGARVGSTPVSGQPGADGMMGTTAAAGGMGSDNRFGYGGAGGASTEAV"
     gene            complement(1016..3055)
                     /locus_tag="VFV99_10710"
     CDS             complement(1016..3055)
                     /locus_tag="VFV99_10710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5059822.1"
                     /translation="MLGSKTLYAALFFTALVAAPTLGEAKGLTVDDMLAMQRVSDPQV
                     SPDGRWIAFAVRDTDYDANRGRYDVWLAAIDGSLVTRLTTHPDNDQDPRWSPDGKWIY
                     FTSTRSGSAQVWRIQPSGGEAEQVTKLDTDINGFKLMPDGKRIILAIDVWPTAKTLAE
                     SIKLDDSKSKSKVKAHATEQLLFRHWDQWEDGKYSHLFMWTSDSDVRDLTPGLMTDTP
                     IHPFGGMEQVTVSPDGKWLAYVARVGGREIAWTTNTDIFLVSTDGKGRALDITADNKA
                     YDFDPAFSPDGKSIAILQMKRPGFEADRQRISLYDLGTKKVRVVTENWDRSAGEIAWS
                     ADAKTIYTSADNIGNVALFAIDLASGSAKTLVEKGTNNSVRVAGNRIVFAKDTLKMPT
                     ELFTMKPDGTDVRQLTKLNADKLAKIQFGEYQQFSFKGAKGDTVYGYSIKPSGYTGGK
                     APVAFLIHGGPQGSFGDHFHYRWNPEFYAGAGYAVVFVDFHGSTGYGQAFTDAISGDW
                     GGAPFEDLMLGLDAALAKFSYLDGTRVAALGASYGGYMINWINGKTDRFKALVCHDGI
                     FDTKFGYYDTEEVWFPEWEHKGTPWDKPEEFEKFSPAANVKNWKTPTLVIHGGLDFRI
                     PESHGIAAFTALQRRHIASRFLQFPDENHWVLKPQNSKLWHQEVLAWIDRFTKKK"
     gene            3075..5012
                     /locus_tag="VFV99_10715"
     CDS             3075..5012
                     /locus_tag="VFV99_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5059823.1"
                     /translation="MSLLRALSVFAVIAACGGAATQKRVRKPGDEYLAAIKFEGVSLS
                     HENLLSGLALKRNLDAGRSIDEYQLNLDVQRIAGLYQRRGFFSVKVVPRVERKGDAAT
                     LIFHVDEGERAVATVVITGLPPEVPFADARAAVPVPNDSPFDYDLFDEGKAPLLALVE
                     NAGYAHAQLDAQVLADRGKGRATLRYALDPGTKVTFGKVTITGVTDALAEAARNRLPF
                     REGDPYSTKQVADAEQAIYGIGRFASARVDVDRMSEATVLPVKVVLTEQKPWEAKLGG
                     GAGFDSLTYQARLRASLTHDGWPTPLTRLGAEFRPALTALRSECKVYEVWNCDFQPRI
                     RLIGTASQQDFLRRDVRADMEGGLDYLKLEAYTTQGERVKLSVGMPLANRRIDVRLGW
                     QFAYYGFDDYALVMDDGSGKFVRDENLVTKTGTASAERLGAFSETVAFDWRDNPITPH
                     LGAYAELRVTQGGSWAGGAFDYVQLMPDLRGYLPLGKAVLAGHVRYGAIFGDVAPTER
                     FYAGGAASQRGFPERYLSPFVTGIDRDDHTKMTNVPIGGAGLLETGLELRVPFELFNT
                     QMGAAAFLDGADVTEQPDDIDPTNLHWAAGISLRPYYLPIGPIRLDFAWRLNRTNSDT
                     AALNPLPGEHFSFVFSLGEAF"
     gene            5012..>6981
                     /locus_tag="VFV99_10720"
     CDS             5012..>6981
                     /locus_tag="VFV99_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059824.1"
                     /translation="MKKVWRWTKRIVLGTLAFVVLATGITLGALHTNWGRNRVRKIVE
                     DQLQSTFPGSTIGKIDGSVLGDLIARDVTIMGRDKRPLVTIKKVTIEAALLPLASKTA
                     RVTKIDAEDVVVYAHDQGPPPPEKPPEPSTPSEPSSWSVELPAIHAHGRIEIETATGV
                     ERIDDLDTMVALSLRSGEPLKASVDTTARWRNQDLAVRAFVEQGDSLTVQYALVTLGS
                     ARVLAANAHVDGTRIDANVFAFAPAALVKQLAQVDLPGNAVVFAQLANTGHLTVDATV
                     GAATARVLAKADLETKAVHAIVTADVPDLAAVVPNRQGEPPIAGSGTVVATVVATPDH
                     VDGIVTIAGEAPRGTGNGLIAISATRERARVLVAATAESPPSRGDRQAPKQDLFAWRL
                     RRLGGSTLIERANDGWFLREGTLEAAATGDAGEVTASLSATGALSPTPSLAVQGTVDG
                     RSVRYNDTAIASLHTRFAATEIPARPTGDVSIEVNGLRQGTLAVPVVTLAAHGTLHED
                     GTIDVDLDRHRVRTADGQQWGGNSGHVRVTEQSIAATQLVTSSGKARITAQAQIGRTS
                     DALTAKLTARDIAVATLDPTLQGTLGADVDVSRRDGMWKGTATIDANQIVVPNRPVLD
                     GKLAVKIDKRRVTAVATASNPAIGSATLDLDV"
BASE COUNT         1181 a         2350 c         2264 g         1186 t
ORIGIN      
        1 tgacggcttc agtgctcgcc ccgccagcac cgccataacc aaagcgattg tcgcttccca
       61 tgccgcccgc tgccgccgtg gtccccatca tcccgtccgc tccaggctgg ccgctgacgg
      121 gcgtactacc gacgcgcgcg ccaccctccc cgccacctcc accgttcgcg ctgatcgctc
      181 cagtcaccac gatcgacggg ctctcgatca cgacgagccc accgctacca ccgccaccgc
      241 caccagcctg gacctggccg ccgttgccgc cagctccggt cgctcggatg acgccgctga
      301 tctggatgct ctccgcggcg tacaggtaca gcgcaccacc actatgaccg ccgctgccac
      361 cgacgccgcc ggtggcatcc tcgttcgcac ctgcctggcc acgacaacca ccgcgcaatg
      421 ccgtgacact cgccggcgca ccggccatgc ctggtgcccc cattccgtcg ctaccgccct
      481 gactctgatc ggtgtctccg gttccaccgt tgccgccggc gaggccgaag cttccgccgg
      541 caccaccgcc cgcgccgccg agatccaatt ctggtgtcgc cgcgaacgaa cacgccgtgt
      601 cgctcgatgc ggggccgcgc tgtccgctga tcctgctcga tacatcaatc gtgccgccca
      661 cggtcaacgc gccggaggcg gcgatcgcga gtggccgggt ccccgtcgca gtcagcgatg
      721 cgccgctgtc gatatcgacc atcgtcgcgt agacaagaca gacgtcgccg ccactcgcct
      781 gcatcacccc gaggcaacgc ggatccgtgt ccgtgttgat cgtgtcggcg ttcacgagat
      841 gcagcggtcc cggcggtgct ggcgatccgc acaggtccag aaacaagccg caccgcccat
      901 caccgtcggg tgttgctata tccccgccgg tcgcgtcgtc gagcgccacg ccatgctcga
      961 acgaacaccc gaccagcagc gcgagtacgg caacacgcac atcccgagtg tatcgctact
     1021 tcttcttcgt aaagcgatcg atccacgcga gcacttcttg atgccagagc ttcgagttct
     1081 gcggcttcag cacccagtgg ttctcatcgg ggaattgcaa gaaccggctc gcaatgtggc
     1141 ggcgctgcaa tgcggtgaac gccgcgatgc cgtgcgactc cgggatgcgg aagtcgaggc
     1201 cgccgtggat gaccagcgtc ggggttttcc agttcttgac gttcgccgcc gggctgaact
     1261 tctcgaactc ctccggcttg tcccacggcg tgcctttgtg ctcccactcc gggaaccaca
     1321 cttcttcggt gtcgtagtaa ccgaacttgg tgtcgaagat gccgtcgtgg caaacgagcg
     1381 ccttgaagcg atcggtcttg ccgttgatcc agttgatcat gtagccgccg tacgacgcgc
     1441 ccagcgccgc gacgcgcgtg ccatcgaggt agctgaactt cgcgagcgca gcgtcgaggc
     1501 cgagcatcag gtcttcgaac ggagcgccgc cccagtcacc gctgatcgcg tcggtgaacg
     1561 cttggccgta cccggtcgag ccgtggaaat cgacgaacac gaccgcgtag ccggcaccgg
     1621 cgtagaactc ggggttccag cggtagtgga agtgatcgcc gaagctgccc tgcggaccgc
     1681 cgtgaatgag gaatgcgacc ggcgccttgc cgccggtata gccgctcggc ttgatcgagt
     1741 agccgtagac ggtgtcgccc ttcgcgccct tgaacgagaa ctgctggtac tcgccgaact
     1801 ggatcttcgc gagcttgtcg gcgttgagct tcgtgagctg acggacgtcg gtgccatcgg
     1861 gcttcatcgt gaacagctcg gtcggcatct tgagcgtgtc tttcgcgaac acgatgcggt
     1921 ttccggcgac gcggaccgag ttgttggtgc ccttctcgac gagcgtcttc gcgctgccgc
     1981 tcgcgagatc gatcgcgaat agcgcgacgt tgccgatgtt gtccgcgctc gtgtagatcg
     2041 tcttggcgtc cgcggaccac gcgatctcgc cggccgagcg atcccagttc tcggtgacga
     2101 cgcggacttt cttcgtgccg aggtcgtaga gcgagatgcg ctggcgatcc gcctcgaagc
     2161 cggggcgctt catctgcagg atcgcgatcg acttgccgtc gggcgagaac gccggatcga
     2221 agtcgtatgc cttgttgtcg gccgtgatgt cgagcgcacg acccttgccg tcggtcgaga
     2281 ccaggaagat atcggtgttc gttgtccacg cgatttcacg gccgccgacg cgcgcgacgt
     2341 atgcgagcca cttgccgtcg ggcgagaccg tgacttgctc catgccgccg aacggatgga
     2401 tcggcgtgtc ggtcatgagg cccggcgtca gatcgcggac gtcgctgtcg ctcgtccaca
     2461 tgaacaggtg cgagtacttg ccgtcctccc actgatccca gtggcggaac aacagctgtt
     2521 cggtcgcgtg cgctttgacc ttcgacttgc tcttcgagtc gtcgagcttg atcgactcgg
     2581 cgagggtctt ggcggtcggc cacacgtcga tcgcgaggat gatgcgcttg ccgtcgggca
     2641 tcagcttgaa gccgttgatg tccgtgtcga gcttggtgac ctgctcggct tccccgccgc
     2701 tcggctggat gcgccagacc tgcgcactgc ccgaccgcgt cgacgtgaag tagatccact
     2761 tgccgtcggg agaccaccga ggatcctggt cgttgtcggg gtgcgtggtt agccgggtta
     2821 cgaggctgcc gtcgatcgcg gcgagccaga catcataacg accgcgattg gcgtcatagt
     2881 ccgtatcgcg taccgcgaat gcgatccatc tgccgtcggg cgacacctgc gggtcgctca
     2941 ctcgctgcat ggcgagcatg tcgtcgaccg tcaggccctt ggcttcgcca agagtgggcg
     3001 cggcgactag tgcagtgaag aacagcgcgg cgtacaacgt cttcgaacca agcatggccg
     3061 cgtactatca cgggatgtcc ctcttgcgag cgcttagcgt gttcgcagtg atagccgcat
     3121 gcggcggcgc ggctacgcaa aagcgggtac gcaagccggg ggacgagtat ctcgcggcca
     3181 tcaaattcga gggggttagc ctcagccacg agaacctcct gtcggggctg gcgctcaagc
     3241 gcaacctgga cgccggccgc tcgatcgacg agtaccagct gaacctcgac gtccagcgca
     3301 tcgccggcct gtaccaacgg cgcggcttct tctcggtaaa agtcgtgccc cgcgtcgagc
     3361 gcaaaggtga cgctgcgacc ctgatcttcc atgtcgacga gggtgagcgc gccgtcgcca
     3421 ccgtcgtgat caccgggctg ccgcccgaag ttccgttcgc ggacgcgcgg gccgccgtcc
     3481 cggtccccaa tgactcgccg ttcgactacg acctgttcga cgagggcaag gcgccgctgc
     3541 tcgcgctggt cgagaacgcg ggttatgcgc acgctcagct cgacgcccag gtgctcgccg
     3601 atcggggcaa aggccgcgcg acgctgcggt acgcgctcga tccgggaacg aaggtcacgt
     3661 tcggcaaggt gacgatcacc ggcgtcaccg acgcgctcgc cgaggcggcg cgcaatcggc
     3721 tgccgtttcg cgaaggcgat ccgtattcga cgaagcaagt cgccgatgcc gagcaggcaa
     3781 tctatggcat cggccggttc gcgtcggcgc gcgtcgacgt cgaccgcatg agcgaagcca
     3841 ccgtgctgcc ggtcaaggtc gtgctgaccg agcagaagcc atgggaagca aagctcggtg
     3901 gtggcgcagg cttcgacagc ctcacctacc aggcccgact gagagcgtcc ctgacacacg
     3961 acgggtggcc gacgccgctg acgaggctcg gcgccgaatt ccggcccgcg ctcacggcct
     4021 tgcgctcaga atgcaaggtc tacgaagtgt ggaactgcga cttccagccg cgcattcgcc
     4081 tgatcggtac cgcctcgcag caagactttc ttcgccgcga cgtcagagcc gacatggaag
     4141 gcggcctcga ttacctcaag ctcgaggcgt acacgacgca gggcgagcgg gtgaagctca
     4201 gcgtcggcat gccgctcgcg aaccggcgga tcgacgtccg gctcggctgg cagttcgcgt
     4261 actacgggtt cgatgactac gcgcttgtca tggacgacgg gtcggggaag ttcgttagag
     4321 acgagaatct cgtcacgaaa accggcaccg ccagcgccga gcggctcggt gcgttcagcg
     4381 agacagtcgc gttcgactgg cgcgacaacc cgatcacgcc gcatctcggc gcgtacgcgg
     4441 agctgcgcgt gacccaaggt ggctcgtggg ccggcggtgc gttcgactac gtgcaattga
     4501 tgccggacct gcgcgggtac ctgccgctgg gcaaggcggt gctcgccggg cacgttcgct
     4561 acggtgcgat cttcggcgac gtcgccccga cggagcggtt ctatgccggt ggcgcggcga
     4621 gccagcgcgg atttcccgag cgctatctgt cgccgttcgt gaccggcatc gatcgcgacg
     4681 accacacgaa gatgacgaac gtgccgatcg gcggcgcggg tttgctcgag accggtctcg
     4741 agctacgcgt gccgttcgag ctgttcaaca cgcagatggg tgcagctgcg ttcctcgacg
     4801 gcgctgatgt caccgagcag cccgacgata tcgatccgac gaacctgcac tgggcggcgg
     4861 gcatcagcct caggccgtac tacctgccga tcggaccgat ccggctcgac ttcgcgtggc
     4921 gcttgaaccg caccaacagc gatactgctg ctctcaatcc gctgcccggc gagcacttca
     4981 gcttcgtgtt cagcctcggt gaggcgttct gatgaagaag gtgtggcgat ggacgaaacg
     5041 gatcgtgctc ggcacgctcg cgtttgtcgt gctcgccacc ggcatcacgc tcggcgcgct
     5101 gcacacgaac tggggtcgca atcgcgttcg caagatcgtc gaggaccagc tgcagtcgac
     5161 ctttccgggc tcgacgatcg gcaagatcga cggcagcgtg ctcggcgatc tgatcgcgcg
     5221 cgacgtcacg atcatggggc gcgacaagcg gccgctcgtc acgatcaaga aggtgacgat
     5281 cgaggcggcg ctgttgccgc tcgccagcaa gaccgcgcgg gtgacgaaga tcgatgccga
     5341 ggatgtcgtc gtgtacgcgc acgatcaggg accgccgccg cccgagaaac cgccggagcc
     5401 gagcacgccg agcgagccga gctcatggtc ggtcgagcta cctgcgatcc acgcgcacgg
     5461 tcgcatcgag atcgagaccg cgaccggcgt cgagcgcatc gacgatctcg acacgatggt
     5521 cgcgctgtcg ctgcggtccg gcgagccgct gaaggcatcg gtcgacacca cggcgcgctg
     5581 gcgcaaccag gacctcgcgg tgcgcgcgtt cgtcgagcaa ggtgattcac tcacggtgca
     5641 gtacgcgctg gtgacgctcg gtagcgcgcg cgtgctcgca gcgaacgcac acgtcgacgg
     5701 cacgaggatc gacgcgaacg tgtttgcatt cgcgccggcg gcgctcgtca agcagctcgc
     5761 gcaggtcgac ttgcccggca atgccgtcgt cttcgcgcag ctcgcgaaca ccggccacct
     5821 caccgtcgat gccacggttg gtgcggcgac tgcgcgggtc ctcgcgaagg cggatctcga
     5881 gacaaaggcc gtgcacgcga tcgtcaccgc ggatgttccg gatctcgcgg ctgtggtgcc
     5941 taaccgccaa ggcgaaccgc caattgcagg cagcggcact gtggttgcga ccgtagtcgc
     6001 tacaccggat cacgtcgacg gtatcgtcac gatcgcaggt gaagcacctc gcggtaccgg
     6061 caacggcctg atcgctatca gcgcaacgcg cgaacgtgct cgcgttctcg tcgcggcgac
     6121 ggcagagtca ccgccaagtc gcggagaccg ccaagccccg aaacaagatc tcttcgcttg
     6181 gcgtctgcgg cgtcttggcg gttcaactct gatcgaacgc gcaaacgacg gttggttctt
     6241 gcgcgaggga acgctcgagg ccgccgcaac cggtgacgca ggggaagtga ccgcgtcgct
     6301 ttcggcgacc ggggccctat caccgacgcc atccttggca gttcaaggca cggtcgacgg
     6361 caggagcgtc cgttacaacg acaccgcgat cgccagcctg catacgcgct tcgcggcaac
     6421 cgagattccg gcgcggccga ccggcgacgt ctcgatcgag gtcaacggcc tccgtcaggg
     6481 cacgctcgcc gttccggtcg tcacactcgc tgcgcacggc acgctgcacg aggacggcac
     6541 gatcgatgtc gacctcgatc gccatcgcgt gcgcaccgcc gacggccagc agtggggcgg
     6601 caacagcggt catgtccgcg tcaccgagca gagcatcgcg gcaacgcagc tcgtgacgtc
     6661 gagcggtaaa gcgcgcatca ccgcgcaagc gcagatcggc cgcacgtccg atgcgctgac
     6721 cgcgaagctc accgcacgcg acatcgcggt cgcgacgctc gatcccacgc tgcaaggcac
     6781 gctcggcgcc gacgtcgacg tgtcgcggcg cgacggcatg tggaagggca ccgcgacgat
     6841 cgacgcgaat cagatcgtgg tgccgaaccg gccggtgctc gacggcaagc tcgcggtcaa
     6901 gatcgacaag cggcgcgtga ctgcggtcgc gaccgcgagc aaccccgcga tcggcagcgc
     6961 gacactcgac ctcgacgtga t
//
LOCUS       DASUFX010000271        11450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1710385, whole genome shotgun sequence.
ACCESSION   DASUFX010000271 DASUFX010000000
VERSION     DASUFX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710385"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFV99_10725"
     CDS             <1..639
                     /locus_tag="VFV99_10725"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEX5059825.1"
                     /translation="VVLGVVGLVKKLDWQQMIIGCFAGLVIVKSWAVVDNLTSKHQAM
                     NRSYDAWALVNTYGAFGSVGDKRYEIVFEGTLSDGPKTGEWREYDLPCKPGRIDRRPC
                     VLGPYHRRLDWLIWFSAMYEQPHDPWLIHLAWKLLDGDRTVRELLIDPFHGTPPKWVR
                     IRRYLYHLQPYSAPTWWTRELVDGEWLQPINKDTPGLKETLARYGWPSPSVH"
     gene            complement(636..1214)
                     /locus_tag="VFV99_10730"
     CDS             complement(636..1214)
                     /locus_tag="VFV99_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059826.1"
                     /translation="MSRVLLVVGVLGLCSAEAMADPQVRFGMTFGVDRNVPEAHEFGP
                     MFGIGMGAGRFTGELNYSYLSMFDQATRVHRAGVTLRMDFVRTSWDMSDQSKAFYGEV
                     GAARRFGYWSVDDLMPVEDKSQNELQLAVGYELANDGGAWQLALRFGFARRDPELGSA
                     CRGVACPVAMPASTGLAESAMLEWTWLLNRGH"
     gene            complement(1289..2800)
                     /locus_tag="VFV99_10735"
     CDS             complement(1289..2800)
                     /locus_tag="VFV99_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5059827.1"
                     /translation="MDRRELLAAERGTLFKEAETRIALVYPSPYRAAMSSLGYQQIYR
                     TLHAMPGVAADRAMLPDEGEDGRFETLETGRPVGSYPLLAYSVAYELEIAGVVETLTR
                     ASIPVLRDERDNRHPIIVGGGPLTFSNPAPLAPFCDVIVVGEGEELIGELVTLAREVG
                     FVRDKLWELLADKPGYYLPHLHGETVPKVAAVDDALLPARSVIATPHTELADMFMTEA
                     ARGCSRGCTYCVMRRSTNGGMRIVPRETIIAGIPDGAKKVGLVGAAVTDHPDIAGIVH
                     DVVTADRQVGISSLRADKLTDELVGLLARGGYRTLTVAADGASERMRRVVERSTQAKH
                     LLRSAELAKAHRLHTLKVYMMLGVPSETDADVDELVQLSKELAAIHPRVAYGLAPFVA
                     KRNTPLDGTDFAGIDVIEARLARLRRGLGAAGLGGKVEVRPTSARWAWVEYMLAQGES
                     SAGLAVMDAHRAGGSFAAYKRAFEQRGVVPTGPRARVPSSQELIALKKKKLAS"
     gene            complement(2800..3411)
                     /locus_tag="VFV99_10740"
     CDS             complement(2800..3411)
                     /locus_tag="VFV99_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059828.1"
                     /translation="MRLGLVLVGVALCAHAAAAPRAGKVVRVERRPSGLTGTPRYCTV
                     STVDMIAYCISEKSPDVGDHLTVIDSQHVLGVIRVTHVQPLPDGCQQNMMWMTQGSLD
                     SGDLSNPQGQMIATLDVPVDARSSKLLGTDKSPGGHPWGTDTIYAVDRNGDGNPDVEF
                     VQYQCDDAGNQSINATATCYEVWAAAAGRGLEKIRAERQKHCY"
     gene            3446..5269
                     /locus_tag="VFV99_10745"
     CDS             3446..5269
                     /locus_tag="VFV99_10745"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5059829.1"
                     /translation="MSSLRYEVRVEAPERHVADIELRFSPDANVVDLTLPAWCPGSYL
                     IRDYARYVRDLEVHTADDTQLEATKIDKQTWRVDTGGAKELVVSYTIYGHDLTVRTNH
                     IDADHAFLHGPATFLYPVSQRNVPVEVTLVFPEPWAMVTAASPVGEISAPAVISKRGT
                     GSRIRTTSIDELFDHPIHLGQTRSYNIRSQVTCNLAIWGERSPGGMFDEQRLATDLAA
                     IVDDHIRRFGEAPFHEYTFLLMLTHEGYGGLEHRASSVNLYNPHFASTRKAYEGLLEL
                     LSHELFHAWNGKRIAPKPLLDFDYTREAYTPCLWVMEGITSHYDRFALRTSGRITAKS
                     YLEKVLDDYARLQATPGRARQSLEESSFDAWIKLYKPDESNLNTTVSYYLKGGLVMFG
                     LDLHIRKRTGNARSLDDVLRLLWKRYGAKGVPHPDDLQPVFEEATDLPLGDIFDAQVR
                     GTEDPNLRADLGFFGLELRTSSDPVTATDGATGCWLGVVTSGTKVTGVFDGSPAQAAG
                     ISPGDELIAIDGFRTTSDNDLRNLLSTRRMSEPVRFSLFRRHRLLELVTRIEHAPPTR
                     YEFATVADPGTGADEYARWLGEPFPGAGQVLATVLTTARWV"
     gene            5288..5950
                     /locus_tag="VFV99_10750"
     CDS             5288..5950
                     /locus_tag="VFV99_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059830.1"
                     /translation="MGRFLVVAVALCAFGACKDKNKSDGLPPAQEWSANAGGAMPPAG
                     PSAQNPHGTNPHAAMGGGDQGGGEGNMPPGHPPIDNPHGGGSPDVAAMGIPGPDPNRK
                     INPNNRVKGVIKVHAKAKDRVKAGGAVFVIVKRSVDGAPSGPPLAVDKLTWDKDELPF
                     EMTEKQAMIAGTELTGEVIVIARYDQDGDAISKQPGDVSGSVKVKIPADKVTLTLDDV
                     LQ"
     gene            complement(5955..6809)
                     /locus_tag="VFV99_10755"
     CDS             complement(5955..6809)
                     /locus_tag="VFV99_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059831.1"
                     /translation="MMGSLRAIACVLIFAPALAAAQPPPTPQQKQAASDKVKKAISKS
                     QGGEHEAAVDLYLEAYAIIPQPLLLSNVGSEYQQMKKPYEALKYFCKYIEADPTGNNV
                     QYATAQARTIYIELGGIPSVEDKDVCKPIVKKQEPPPPPPNPVLTNPEPTPGPAPGPV
                     DNGPKKPTPTLRYVGVGVAVVGAAVFGVGTYYGLQAKSISDEITNHPTNEPWRSDIKE
                     FEAEGKSDEKKQIMFMVGGGLALAAGVTLFIVGGSKSSSESSTSASLSIAPVATPDQL
                     GFAAAGRF"
     gene            complement(6829..7677)
                     /locus_tag="VFV99_10760"
     CDS             complement(6829..7677)
                     /locus_tag="VFV99_10760"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5059832.1"
                     /translation="MATTNLPGTSGELGSELVAALELARDAGVEIMKLRGGELGVEMK
                     KGDEPVTIADKRANQLIVDGLAARFPDDPIISEELPSAEATLRAPRLWLVDPIDGTKD
                     FIRGSDGFSVMIGIVLDGRPSLGVVYQPAIDRTFFATPDGGAHVVTGDQVTPLVVSDV
                     ATAADVRLVASASHRSADIDRVKTTLGIDNELNVGSVGVKLCLIAMGVRDLYVNPAAK
                     TKVWDTCGPEAILTRAGGRLSDLFGEPVDYTELRQRRGLVASNGRVHDEVVAKLAPLF
                     EQLRRK"
     gene            complement(7664..8521)
                     /locus_tag="VFV99_10765"
     CDS             complement(7664..8521)
                     /locus_tag="VFV99_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5059833.1"
                     /translation="MSTDKVIISCALTGAVTTKKHCPAIPYTPVEIAEEAKRAYDAGA
                     AIVHIHARNDDGSPTWSKDTFQAIMNETKARCPILINWSTGGMGPMQDRVAHLAQRPH
                     VAALNMGSMNYAKWNAEKKQFAFNFVFTNSFDDIVAFAKAMKEHNVKPEMECFDVGHT
                     NSHRVLADMGLLAAPFHFSFIMGVVGGIPASARHLAFQAEQVPAGSRWKVIGISRDQW
                     PLAMAALALGGDVRVGLEDNFYLPDGTMAKSNGDLVAAAVALAKLSGRSVASVDEAKH
                     LLWPNGGHN"
     gene            complement(8613..9044)
                     /locus_tag="VFV99_10770"
     CDS             complement(8613..9044)
                     /locus_tag="VFV99_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX5059834.1"
                     /translation="MIEEVRVRPLATPALLLGIGLGGVIDGIVIGWSGILLAVTWVLA
                     VAGVILLFRAGRRDDVVWSWRIFIGTMLLGWGLFNVVEGTIDQLVLGIHHTHPGGNTF
                     VWDLGFVIIGGVALMLIGYLIARTELSGSRMSPATRRALGI"
     gene            9262..10509
                     /locus_tag="VFV99_10775"
     CDS             9262..10509
                     /locus_tag="VFV99_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059835.1"
                     /translation="MTKVLGLALVAVVAACQPPKKPVAKPVAVTARPFDRGPTATLVD
                     QNQPGPSYRVQIAADITRDVMPTDDLAIVVMVNDEEVGRYPIVGKFEQRPTRIKQEIE
                     LPLGDYEIEFNYQGTRYAGSPFRLAEVPVWGGRRALQLRSHPGTRISLREKKLWVGRW
                     WANDGLPQAWIVEWVREGQVVTMTSGREEHGIPPQGARLVGGANAAWHDEKLVNNTIW
                     TYGEEYGVPDVVMQQPGVWAARCVHGNSPPVAVVFHVLPGGALAELSSRKIASVGWED
                     SWSKPLEARALSVTEVDRLSSKLPKISASEPFDEATAQQGIEPSIRLSLSAVRALFRS
                     KQLVEAWSTYNTLNQPSSYVQLHPGQTPAAAGKRNGVATPQRETPGKENERRAKLKQL
                     RTQIEGLIKANGAPWKAEEYPHS"
     gene            complement(10599..>11450)
                     /locus_tag="VFV99_10780"
     CDS             complement(10599..>11450)
                     /locus_tag="VFV99_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059836.1"
                     /translation="ADAPPDARMCLSRATYPLTITAGAFPASPDHPSVIVVVPQGFDP
                     TPPLDLVVYIHGFNNCITNVVGDTNTACAPNTGIRQASSLATQLDASGRNALLVVPEV
                     MYDQASGDPGQLGVTDGMKALLTETLASVPAPLGPLDLASHGRVIVATHSGGYQAASA
                     MITFGGVPVDEVWLLDSLYGYTTRFDAWVMKDIASLASTRRFVDLYTSGGGTDQNSIA
                     MASRAASWVTADPSVLIDDQSTTPITDDALAHGLVFKHVAETHNDVPRVYMERLLATS
                     KLAPVCN"
BASE COUNT         1954 a         3706 c         3870 g         1920 t
ORIGIN      
        1 gtcgtcctcg gcgtggtcgg tctcgtaaag aaactcgact ggcagcagat gattatcggc
       61 tgttttgccg gcctcgtgat cgtgaagagc tgggcggttg tcgacaacct gacctcgaag
      121 caccaggcga tgaaccgcag ctacgatgcg tgggcgctcg tcaacacgta cggcgcgttc
      181 ggctcggtcg gcgacaagcg ctacgagatc gtgttcgagg gcacgctgtc cgatggcccg
      241 aagaccggcg agtggcgcga gtacgacttg ccgtgcaagc ccgggcgcat cgatcgccgg
      301 ccgtgcgtgc tcggtccgta tcaccgccgg ctcgactggc tgatctggtt ctcggcaatg
      361 tacgagcagc cgcacgaccc gtggctgatc cacctcgcgt ggaagctgct cgacggtgat
      421 cgcaccgtgc gcgagctatt gatcgatccg tttcacggca cgccgccgaa gtgggtgcgg
      481 atccgccgct acctctatca cttgcagccg tacagcgcgc cgacgtggtg gacgcgcgag
      541 ctcgtcgatg gcgagtggct gcagccgatc aacaaggaca ccccaggcct caaggagacg
      601 ctcgcgcgct acggctggcc gtcgccgtcc gttcactagt ggccgcggtt caggagccac
      661 gtccactcga gcatcgcgga ctcggcgagg ccggtcgacg ccggcatcgc aacggggcac
      721 gcgacgccgc ggcacgcgga gccgagctcc ggatcgcgac gcgcgaagcc gaacctgagc
      781 gcgagctgcc acgcgccgcc gtcgtttgct agctcgtagc ccactgcaag ctggagctcg
      841 ttctgcgact tgtcctcgac gggcatcaga tcgtcgacgc tccagtagcc gaaccggcgc
      901 gcggcaccga cctcgccgta gaacgctttc gactggtcgc tcatgtccca ggacgtgcga
      961 acgaagtcca tgcgcagcgt gacgcccgca cgatgcacgc gcgtcgcctg atcgaacatg
     1021 ctgaggtacg agtagttcag ctcgccggtg aaccgcccgg cgcccatgcc gatgccgaac
     1081 atcgggccga actcgtgggc ctcggggacg ttgcggtcga caccgaacgt catgccaaag
     1141 cggacctgcg gatcggccat cgcctccgcg ctgcacaacc ccaggacgcc gacgacgagg
     1201 agcacgcgcg acatggatca aagcctaccg gagcgcgatc acgcggcaag gcagaatctc
     1261 tggcacctga tcgattaccg ggagtcgctc agctcgcgag cttcttcttc ttcagcgcga
     1321 tcagttcctg actcgatggc acacgcgcgc gtggaccggt tggcacgacg ccgcgctgct
     1381 cgaacgcgcg cttgtaggcc gcgaagctgc cgcccgcgcg atgcgcgtcc atcaccgcga
     1441 gaccagccga gctttcgccc tgcgcgagca tgtattcgac ccatgcccag cgcgccgatg
     1501 tcgggcggac ctcgaccttg ccaccgagtc ccgcggcacc gaggccgcga cgtagccgcg
     1561 cgagccgtgc ctcgatgaca tcgatgcccg cgaagtcggt gccgtcgagc ggcgtattgc
     1621 gctttgcgac aaacggcgcg agcccgtacg cgaccctcgg gtggatcgcg gccagctcct
     1681 tcgacagctg gaccagctcg tcgacatcgg cgtcggtctc gctcggtacg ccgagcatca
     1741 tgtagacctt cagcgtgtgg agacggtgcg ccttcgcgag ctcggcggag cgcaataggt
     1801 gcttggcctg cgtagaacgc tcgacgacgc gtcgcatgcg ctcactcgcg ccatcagctg
     1861 cgaccgtcag cgtgcgatag ccgccgcgcg cgagcaggcc gacgagctcg tcggtgagct
     1921 tgtccgcgcg caggctcgag atgccgacct gacgatccgc ggtgacgacg tcgtgcacga
     1981 tgccggcgat gtcgggatga tcggtgaccg ccgcgccgac gagaccgacc ttcttcgcgc
     2041 cgtcggggat gcccgcgatg atcgtctcgc gcgggacgat gcgcatgccg ccgttcgtcg
     2101 agcgccgcat cacgcagtac gtgcagccgc gcgagcagcc gcgcgccgct tcggtcatga
     2161 acatatcggc gagctcggtg tgcggcgtcg cgatcaccga gcgggcaggc agtagcgcgt
     2221 cgtcgacggc ggcgaccttc ggtacggtct cgccgtgcag gtgtggcagg tagtagccgg
     2281 gcttgtcggc gagcagctcc cacagcttgt cgcggacgaa gccgacttcg cgcgcgagcg
     2341 tgacgagctc gccgatcagc tcttcacctt cgccgacgac gatcacgtcg cagaacggcg
     2401 cgagcggtgc aggattcgag aacgtgagcg gaccgccgcc gacgatgatc ggatgacggt
     2461 tgtcgcgctc gtcgcgcagg accgggatgc tcgcgcgggt gagcgtctcg acgacgccgg
     2521 cgatctcgag ctcgtacgcg accgagtacg cgagcagcgg atagctgccg actgggcgcc
     2581 cggtctcgag cgtctcgaag cggccgtcct cgccctcgtc ggggagcatc gcgcgatcgg
     2641 ccgcgacacc cggcatcgca tgcagcgtgc gatagatctg ctgataaccg agcgacgaca
     2701 tcgccgcgcg atacgggctc ggatagacga gcgcgatccg cgtctcggcc tctttgaaca
     2761 acgtgccgcg ttcagcggcg aggagctcgc gacggtccat cagtaacagt gcttctgccg
     2821 ctccgcgcgg atcttctcga gaccgcgacc tgccgcggcg gcccacacct cgtagcacgt
     2881 cgcggtggcg ttgatcgatt ggttaccggc gtcgtcgcac tggtactgga cgaactcgac
     2941 gtcggggttg ccgtcgccat tgcgatcgac ggcgtagatg gtgtcggtgc cccacggatg
     3001 accgccgggc gacttgtcag tgccgagcag cttgctcgac ctcgcatcga cgggcacgtc
     3061 gagcgtcgcg atcatctgac cctgcggatt cgagaggtcg ccgctatcga gcgatccctg
     3121 cgtcatccac atcatgttct gctggcagcc gtccggcagc ggctggacgt gagtcacgcg
     3181 gatcacgccg agcacgtgct gactgtcgat cacggtgagg tgatcgccga cgtcgggcga
     3241 tttttcgctg atgcagtagg cgatcatgtc gacggtcgat accgtgcagt accgcggagt
     3301 cccggtcagt ccgctcgggc ggcgctccac gcggaccacc ttgccggcac gcggcgccgc
     3361 ggccgcatgc gcgcaaagtg ctactccgac gaggaccagg cccagacgca cccgtagagc
     3421 atagcagctg tgctacgtac cgagcatgtc gtctcttcgg tacgaggtcc gcgtcgaggc
     3481 gcccgagcgc catgtcgccg atatcgagct gcggttttca ccggatgcga acgtcgtcga
     3541 cctgacgctg ccggcctggt gcccgggaag ttatctgatt cgcgactacg cccggtacgt
     3601 gcgcgatctc gaggtccaca ccgccgatga cacgcagctc gaggcgacga agatcgacaa
     3661 gcagacctgg cgtgtcgaca ccggcggcgc gaaggagctg gtcgtctcgt acacgatcta
     3721 cgggcacgac ctgaccgtgc gcacgaatca catcgacgcc gaccacgcgt tcctccacgg
     3781 gccggcgacg ttcctctacc cggtgagcca gcgcaacgtg cccgtcgagg tcacgctcgt
     3841 gttccccgag ccgtgggcaa tggtcacggc ggcgtcaccg gtcggtgaga tctcggcgcc
     3901 ggcagtgatc agcaagcgcg gcaccggcag tcggatccgc acgacgtcga tcgacgagct
     3961 gttcgatcat ccgatccacc tcggccagac gcggtcgtac aacatccgat cacaggtaac
     4021 gtgcaatctc gcaatctggg gcgagcgctc accgggcggc atgttcgacg agcagcggct
     4081 cgcgaccgac ctcgccgcga tcgtcgacga tcacatccgt cgcttcggcg aggcgccgtt
     4141 ccacgagtac acgtttctgc tgatgctgac gcacgagggt tacggcgggc tcgagcatcg
     4201 cgcgtcgagc gtgaacctct acaacccgca cttcgcgtcg acgcgaaaag cgtacgaggg
     4261 gctgctcgag ctgctgtcgc acgagctgtt tcacgcgtgg aacggcaagc gcatcgcgcc
     4321 caagccgctg ctcgacttcg actacacgcg cgaggcgtac acgccgtgcc tgtgggtgat
     4381 ggagggcatc acgagccact acgatcggtt cgcgctgcgc acgagcggcc gcatcacggc
     4441 gaagagctat ctcgagaagg tgctcgacga ctacgcacgc ctgcaggcga cgcccggccg
     4501 cgcgcgccag agcctcgagg agtcgtcgtt cgacgcatgg atcaagctct acaagcccga
     4561 cgagtcgaac ctcaacacga cggtcagcta ctacctcaag ggcggcctcg tgatgttcgg
     4621 gctcgatctc cacatccgca agcgcaccgg caacgcgcgc tcgctggacg atgtgttgcg
     4681 cctgctgtgg aagcggtacg gcgcgaaggg cgtgccgcat cccgacgatc tgcagccggt
     4741 gttcgaagaa gcgaccgacc tcccgctcgg cgacatcttc gacgcccagg tccgcggcac
     4801 cgaggatccg aacctgcgcg ccgacctcgg cttcttcggg ctcgagctac ggacgtcgtc
     4861 cgatccggtg accgcgaccg acggcgccac cggctgctgg ctcggtgtcg tgacgagcgg
     4921 tacgaaagtc accggtgtct tcgacggctc acctgcgcag gctgcaggca tttcgcccgg
     4981 cgacgagctg atcgcgatcg atggatttcg cacgacgtct gacaacgacc tgcgcaacct
     5041 gttatcgacg cgcaggatga gcgagccggt ccggttctcg ctgttccgtc gccaccgctt
     5101 gctcgagctc gttacacgga tcgaacacgc gccgccaacg cgctatgaat tcgccactgt
     5161 cgccgatccc ggtaccggcg ccgacgaata cgcgcgctgg ctcggcgagc cgttcccggg
     5221 cgcggggcaa gtacttgcta ccgtgctgac gacggcgcgc tgggtttgag ctgctagact
     5281 cgccggcatg ggtcggttcc tcgtagttgc cgttgcgctc tgcgcgttcg gcgcgtgtaa
     5341 agacaagaac aagtcggatg gcctgccgcc tgcgcaggaa tggtccgcga atgcaggtgg
     5401 cgcgatgccg ccagccgggc cgagcgcaca gaatccgcac ggcaccaatc cgcacgcggc
     5461 catgggcggc ggcgatcagg gtggcggcga gggcaacatg ccgcccggcc atccgccgat
     5521 cgacaacccg cacggcggtg gcagccccga tgtcgctgcg atgggcattc cgggacccga
     5581 tccaaatcgc aagatcaatc cgaacaaccg cgtcaaaggc gtcatcaagg ttcacgcgaa
     5641 ggcgaaggac cgcgtgaagg cgggcggtgc ggtgttcgtg atcgtgaagc gctccgtcga
     5701 cggtgcgccg tcgggtccgc cgctcgcggt cgacaagctc acctgggaca aggacgagct
     5761 gccgttcgag atgaccgaga agcaagcgat gatcgcgggc acggagctga ccggcgaggt
     5821 catcgtgatc gctcgctacg atcaggacgg cgacgcgatc tcgaagcagc ccggcgacgt
     5881 cagtggctcg gtgaaggtga aaatccccgc cgacaaagtc acgctgacgc tcgacgacgt
     5941 cctgcagtag cgcgctagaa gcggccggct gcggcgaagc cgagttgatc gggcgtcgcg
     6001 acgggcgcga tcgagagcga ggcggacgtc gagctttcgc tcgatgattt cgagccgccg
     6061 acgatgaaca gcgtcacgcc ggctgcgagc gcgagcccac cgccgaccat gaacatgatc
     6121 tgcttcttct cgtcgctctt gccttccgct tcgaactcct tgatgtcgga gcgccacggc
     6181 tcgttcgtcg gatggttcgt gatctcgtcg ctgatgctct tggcctgcag gccgtagtac
     6241 gtgccgacgc caaacaccgc ggcgccgacg acagcgacgc caacaccgac gtaacggagc
     6301 gtcggcgtcg gcttcttggg gccgttgtcg acggggccgg gggcagggcc gggcgtgggc
     6361 tcggggttgg tgaggaccgg attcggtgga ggcggcggcg gctcctgctt cttcacgatc
     6421 ggcttgcaga cgtctttgtc ttcgaccgac ggaatgccgc cgagctcgat gtagatcgtg
     6481 cgcgcttgcg cggtcgcgta ctggacgttg ttaccggttg ggtccgcctc gatgtacttg
     6541 cagaagtact tgagcgcctc gtacggcttc ttcatctgct ggtactcgga gcccacgttc
     6601 gagagcagca gcggctgcgg gatgatcgcg tatgcctcga gatagagatc gacggcggct
     6661 tcgtgctcgc cgccttggct cttcgagatc gccttcttca ccttgtcgct cgcagcttgt
     6721 ttctgctgcg gcgtgggtgg cggctgcgcg gctgctagcg cgggtgcaaa gatcagcacg
     6781 caagcgatcg cacgaaggct ccccatcatg gaaccaatgg taacacggtc acttgcggcg
     6841 caactgctcg aataatgggg ccagttttgc gacgacttcg tcatggacgc gcccattgga
     6901 cgcgaccagg ccgcggcgct ggcgcagctc ggtgtaatcg acgggttcgc caaacaggtc
     6961 ggacagccgg cccccggcgc gcgtgaggat ggcctcgggg ccgcaggtat cccacacctt
     7021 ggttttcgcg gccgggttga cgtagaggtc gcgaacgccc atcgcgatca ggcacagctt
     7081 cacgccgacc gagccgacgt tgagctcgtt atcgatgccg agcgtggtct tcacgcgatc
     7141 gatatcggcg ctgcgatgcg atgcgctggc cacgagtcgc acgtcggcag cagtcgcgac
     7201 gtccgacacg acgagcggcg tgacctggtc gccggtcacg acatgcgcgc cgccgtcggg
     7261 cgttgcgaag aacgtgcgat cgatcgcggg ctggtacacg acgcctaagc tcgggcggcc
     7321 atcgagcaca atgccgatca tcaccgagaa gccatcgctg ccgcggatga agtccttcgt
     7381 gccgtcgatc ggatcgacga gccacaggcg tggcgcgcgg agcgtcgctt cggcgctcgg
     7441 caactcctcg gagatgatcg ggtcgtcggg aaaccgcgcg gccagaccgt cgacgatgag
     7501 ctggttcgca cgcttgtcgg cgatcgtgac cggctcgtcg cccttcttca tctcgacgcc
     7561 gagctcgccg ccgcgcagct tcatgatctc gacgccggcg tcgcgcgcca gctcgagggc
     7621 cgccacgagc tcgctgccga gctcgccgct ggtaccgggc aggttagttg tggccaccgt
     7681 ttggccagag caggtgcttc gcctcgtcga cgctggcgac gctgcggccc gacaacttag
     7741 cgagcgcgac ggcggccgcg accagatcgc cgttcgactt cgccatcgtg ccatcgggca
     7801 ggtagaagtt gtcctcgagc ccgacgcgga catcgccgcc gagcgccagt gctgccatcg
     7861 cgagcggcca ctgatcgcgg ctgataccga tcactttcca gcgcgagccg gcggggactt
     7921 gctcggcctg gaacgcgagg tggcgtgcgc tcgccggaat gccgccgacc acgcccatga
     7981 tgaacgagaa gtggaacggc gccgcgagca gacccatgtc ggcgagcacg cggtgcgagt
     8041 tggtgtgacc gacgtcgaag cactccatct cgggtttgac gttgtgctcc ttcattgcct
     8101 tcgcgaacgc gacgatgtcg tcgaacgaat tcgtgaacac gaagttgaac gcgaactgct
     8161 tcttctcggc gttccacttc gcgtagttca tcgagcccat gttgagcgcg gcgacgtgcg
     8221 gacgctgcgc gaggtgcgcg acgcggtcct gcatcggtcc catgccgccg gtcgaccagt
     8281 tgatcaggat cgggcagcgc gccttcgtct cgttcatgat cgcctggaac gtgtctttgc
     8341 tccacgtcgg cgagccatcg tcgttgcgcg cgtggatgtg cacgatcgcg gcgccggcgt
     8401 cgtaggcacg tttcgcttct tcggcgatct cgaccggggt gtacggaatc gccgggcagt
     8461 gcttcttcgt cgtcacggcg ccggtcagtg cgcacgagat gatgaccttg tcggtggaca
     8521 cggctggagc atcgacgacg aatcgtcggc gagcaagggg caagtcggtt acatgacgcc
     8581 gggcgagcag gcgtcattac gtacaactgc cgctagattc cgagcgcgcg ccgtgtcgct
     8641 ggtgacatgc gcgagccact gagctcggtg cgcgcgatca agtagccgat gagcatcagc
     8701 gcgacgccgc cgatgatgac gaagccgaga tcccagacga acgtgttgcc gccgggatgc
     8761 gtgtgatgaa tgccgagtac gagttgatcg atggtgcctt cgacgacgtt gaagagaccc
     8821 caaccgagca acatcgtgcc gatgaagatg cgccacgacc acacgacgtc gtcgcgccgt
     8881 cccgcgcgga acagcaggat cacgccggcg accgcgagca cccaggtgac tgcgagcagg
     8941 atgccgctcc aaccgatcac gatgccgtcg atgacgccgc cgaggccgat gccgagcagg
     9001 agcgcgggcg tggccagcgg gcggacacgt acttcttcga tcatttagag ggggatcggt
     9061 gcacccgggg tgcccggcca agggcgcgaa cggccgggac cagaccgggc gccgaaggcg
     9121 cagtgaggcg tgttcgttac tttttgtgcg acctgcgccg cgctcccggc tccacccaac
     9181 gtgacggcgc aaatccggca aaacgtggct cacaaaattg tgatgttcga gctgcgcaat
     9241 tcgtgtaaga ccccggcccg tatgacgaag gtgctgggac tagctttggt tgcagtggtt
     9301 gcggcgtgtc agccgccgaa gaaaccggtc gcgaaaccgg tcgcagtaac ggcccgacca
     9361 tttgatcgtg gtccgaccgc tacgctggtc gatcagaacc agccggggcc gtcgtatcgc
     9421 gtgcagatcg ccgccgacat cacacgcgac gtgatgccga ccgacgacct cgcgatcgtc
     9481 gtgatggtga acgacgaaga agtcggtcgc tatccgatcg tcggcaagtt cgagcagcgg
     9541 ccgacgcgca tcaagcaaga gatcgagttg ccgctcggcg actacgagat cgagttcaac
     9601 tatcagggca cgcgctacgc gggctcgcca ttccggctcg cggaagtgcc ggtgtggggc
     9661 ggtcgtcgcg cgctgcagct gcgctcgcat ccgggcacgc gcatctcgct gcgcgaaaag
     9721 aaactgtggg tcggtcgttg gtgggcgaac gatggtctgc cgcaagcgtg gatcgtggag
     9781 tgggtgcgcg aaggtcaggt cgtgacgatg acgtcgggcc gtgaagagca tggcatcccg
     9841 ccgcaaggtg ctcgcctggt cggtggcgcg aacgcggcgt ggcatgacga gaagctcgtc
     9901 aacaacacga tctggacgta cggcgaagag tacggcgttc ccgatgtcgt gatgcaacag
     9961 cccggcgtgt gggccgcgcg ctgcgtgcac ggcaactcgc cgccggtcgc ggtcgtgttt
    10021 cacgtgctgc ccggtggtgc gctcgccgag ctgagctcgc gcaagatcgc gagcgtcggc
    10081 tgggaagact cgtggagcaa gccgctcgag gcccgcgcgc tgtcggtgac ggaggtcgat
    10141 cggctgtcgt cgaagctgcc gaagatcagc gcgagcgagc cgttcgacga ggccacggcg
    10201 cagcaaggca tcgagccctc gatccggctc tcgctatcgg cggtgcgcgc gctgttccgc
    10261 agcaagcagc tcgtcgaggc gtggagcacg tacaacacgc tgaaccagcc gtcgtcgtac
    10321 gtacagctcc atccgggcca gacccccgcc gccgcaggca agcgtaatgg cgtcgcgacg
    10381 ccgcaacgcg agactccggg taaggagaac gagcgccgcg caaagctcaa gcagctgcgc
    10441 acgcagatcg aaggcctcat caaggcgaac ggcgcgccct ggaaagccga agagtacccg
    10501 cacagctagt tgtccgtagt tcggacaggg tcagacatta cttgtatgcg ttcatcggcc
    10561 gagctgtccg agcaacgcat acaacgcatg tctgacccct agttgcagac gggcgctagc
    10621 ttgctcgtcg cgagcaggcg ctccatgtac acgcgcggca cgtcgttgtg cgtctcggcg
    10681 acgtgcttga acacgaggcc gtgggcgagt gcgtcgtcgg tgatcggcgt cgtgctctga
    10741 tcgtcgatca agacgctcgg atcggcggtg acccagctcg cggcgcgact cgccatcgcg
    10801 atgctgttct ggtcggtgcc gccacccgag gtgtagagat cgacgaaccg gcgcgtgctc
    10861 gcgagactcg cgatgtcctt catcacccac gcatcgaagc gcgtcgtgta gccgtagagc
    10921 gaatcgagta gccacacctc gtcgactggt acgccgccga acgtgatcat cgccgacgcg
    10981 gcctgatagc cgcccgaatg cgtcgcgacg atcacgcggc cgtggcttgc gagatcgagt
    11041 ggaccgagcg gcgcgggcac cgaggcaagc gtctcggtga gtagcgcttt catgccgtcg
    11101 gtgacaccga gctgtcctgg atcgccgctc gcctggtcgt acatgacctc ggggacgacg
    11161 agcagcgcgt tgcgaccact cgcgtcgagc tgggtggcga gcgacgacgc ttgccggatg
    11221 ccggtgttcg gcgcgcacgc cgtgttcgtg tcgccgacga cgttcgtgat gcagttgttg
    11281 aagccgtgga tgtagacgac gagatcgagc ggcggcgtcg ggtcaaaccc ttgcggcacg
    11341 acgacgatca cgctcgggtg atcgggcgat gccggaaacg cgcccgcggt gatcgtgagt
    11401 gggtaggtcg cgcgcgatag acacatccgt gcgtccggag gtgcgtcggc
//
LOCUS       DASUFX010000272         7919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_459971,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000272 DASUFX010000000
VERSION     DASUFX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459971"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFV99_10785"
     CDS             complement(<1..737)
                     /locus_tag="VFV99_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059837.1"
                     /translation="MRLCVVMLAVIACRGGDKPAQKAEPAAAAAPTPEIAAAAATASS
                     DMDPGHCGQQPFAESTPLPEASAAAWLAIDGKLALVIVSDSGNNGAYAVLDPDTGVTR
                     EQGKLPLGTGASDDIEGFAAHDGKLFGLTSDGWMRVWQRKGSGFDLVEGPYAIGTQAD
                     GTTCPTKKKCAINYEGLALATTPQGCAGFACSKGEGRVYCLTEQGGHYAIDRSRSIAV
                     TKPNALGDCTFDDRDGLWVGDNVFGLD"
     gene            767..1393
                     /locus_tag="VFV99_10790"
     CDS             767..1393
                     /locus_tag="VFV99_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX5059838.1"
                     /translation="MPEPRRYLVKEMFGPTLQGEGAHAGRPCVFLRFAACNLDCKWPC
                     DTDFSVEGAVRHTAEEIVAKLLELDTNRARMVVVTGGEPTLQWDAPLAEALHVAGFRI
                     HMESNGTRPLAASVDWLTVSPKPQFHPASISLVTDLPVSELKVVVDDTVDEATLDRYV
                     ERYRCTHYFAQPCMNDQYQHHLQRTLALVLKRPQWRMSVQLHKIIGVP"
     gene            1493..2452
                     /locus_tag="VFV99_10795"
     CDS             1493..2452
                     /locus_tag="VFV99_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX5059839.1"
                     /translation="MLALDQASQFAQLALACVRRELPHKLDHLLLAGTTPSPRELHPA
                     FYGCLDWHSAVHGHWMLARLLEKFPTLPEAAAIRATFDDTLTAENLATEAAYFTDRPS
                     FERMYGWAWLLKLACDLPAPWRAPLQPLVDVIVESYRAFLPKQTYPVRTGVHSNTAFG
                     LTFALDYARAVSDQALEQLIVDRSVAYYGGDVSAPSAWEPSGEDFLSPSLVEADLMRR
                     VVPDFPTWFVRFIPTLSPSLRSPAVVSDRSDPKLAHLDGLNLSRAWCLRNIAEALPPT
                     PLRDELEVAAAAHARDGLKHVATGQYMGEHWLASFAVYLEVPA"
     gene            2449..3570
                     /locus_tag="VFV99_10800"
     CDS             2449..3570
                     /locus_tag="VFV99_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059840.1"
                     /translation="MTLLVARMVEERFHRDTEGRPIREGRTPPEFLQAGEIEFRTCPY
                     SGSRFQHKNPMNVSALRQMGAHWDELCDALAFLRAAHDEVLGVTQPQLMDVWRASQLG
                     SALPWFFLFRDAEIPPYAAALAKATLGMGIWAQRELLQTIATARPAPALTPESILAMA
                     EANGTLVGETEVCSGGDKMLLKFFEVLVGPRAPGRFAAPRDEVLRFGAHYTNFKLYIW
                     LYYLARRFLLADAVAAHGSSPEAAALLDASVEPSDFFRIEPANLAAVIPEQRMGWFRS
                     LADLIVPFAPARTDLALRDLAFQLAAAMGKGAGPTETFAVLDELYGKVIANVEAGFRG
                     GVGTSTADIDVPADIRDRLVASSPRQFFASLPSAPPPDR"
     gene            complement(3567..4130)
                     /locus_tag="VFV99_10805"
     CDS             complement(3567..4130)
                     /locus_tag="VFV99_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059841.1"
                     /translation="MAEVARTRTTFKQRMQMAGVIAGGILTFNTLFWVASYFYFKDKP
                     LEAADTGSVRFAFLQLSLIVAGMAYAAALAPRLIGHGIAIAMGLAAIVGGISALSKGM
                     PPVMGMTMLVVGCALPWLTWMSLQHKRAAWSFLIAVVSVFGAVTFFGAPKVNHILGVG
                     LWHAMIIPGLQIVCVIALSMLRGEYRD"
     gene            complement(4146..5351)
                     /locus_tag="VFV99_10810"
     CDS             complement(4146..5351)
                     /locus_tag="VFV99_10810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059842.1"
                     /translation="MVSERPSQEGKLSPGAPNDPVPFGKYYLLGLIARGGMAEVYRAR
                     IANGDKNLFAVKVMRPQLAREARFIDMFHREGKLAMMLKNRCIVETLEIGQYDGRHYI
                     AMEYIGGRDLTQVLRRCQETQQRIPVPHAVYIAARIAEGLHFAHTLQGPDGRPLNIVN
                     RDVSPSNVRMSYDGDVKLLDFGIAQALMKFTSEIGILKGKFSYMSPEQIRGMPLDART
                     DVFSAGIILHEMLTTEKLFRGDTEFALMEKVRKAEVPPPSNFNRRVTPELDAIVFKAL
                     ARDVADRYQSAAQLAADLDALIAGYRFDPKELRQFIRQLFRKEYQKELEETQMALEAE
                     PGLSFAASIPGYMPKTPTGQSEPETPTATMPSVQVSKEEEPSVRIEGAGNNGDKPKGF
                     WGSLFKRKK"
     gene            complement(5364..6515)
                     /locus_tag="VFV99_10815"
     CDS             complement(5364..6515)
                     /locus_tag="VFV99_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059843.1"
                     /translation="MNWVLRATPVVALLLFAPVARADGEDKPPDKTDVKPPDQAELYS
                     TVTTKKSRAALSQHLVKGAEKAVKDRKWAVAIPLYQALAVARGPASPEAKQLATLWTL
                     AGQNQEAAEAWSNYAAAVKDPKAKAEATAEATRLAGTPDPFADKLALTEMSKEANQAF
                     KQGRSEFKAGRYADALVLFHMGYALAPELPGFLRELGSTYDKLGEGERKKDFYRRYLV
                     QRPFGPNADVVRSELAREKDVLGTLLVSASMPCGEMWVNRQRISGKLPEKGLLVAPGT
                     YKGLCFNPKYEMAMFEYTTVEPGKPSTMTFRWAIVVNKLEKPYGRIALEMASSPGVMN
                     DLGITSLEVGVPVPSDGHKLRMVLKDDSGVRTEERSVQIEPGQKLVVQW"
     gene            6582..7658
                     /locus_tag="VFV99_10820"
     CDS             6582..7658
                     /locus_tag="VFV99_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059844.1"
                     /translation="MRATTLREYAAAHFGSEQPTCKVAGRTHITGTAKNGLSTIVCIM
                     PTRGTLDVLHVVILERNKQVGRKVARLFLSVGATGVVVEEPKHVAPVIEDADVLCADA
                     FDGDFVAEAVRSRPRLRGVLWTAEPLRRALKFLVETRAIDNVLGRKDFDSAPRSWEVL
                     MVARRLGGAPPAPLSAYLDWGFSHLDLDVRTTADRDLSVVKIQDFVAGLQLPKRVGEM
                     FGELAHELLMNAMYDAPVDAQGKAKYAADRKADIRLGDNERPTVRVATDGTRLVLQVR
                     DPFGRLERRHVFDGLARGLAGGEMDQSHGGAGLGMMVCHNSSSAMFFDVVRGRSTEVT
                     ALFELDMNLRELRTQAKSLHFWSA"
     gene            7655..>7919
                     /locus_tag="VFV99_10825"
     CDS             7655..>7919
                     /locus_tag="VFV99_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059845.1"
                     /translation="MMEAVRKSQPAMVSPGRNSGAPLGAPQEPASAAKLTIEKFADGA
                     ISCLKFSGTIDESFEGKKVARSVQGDTLVLDLGGVKKISSFGIR"
BASE COUNT         1293 a         2576 c         2571 g         1479 t
ORIGIN      
        1 tgatccaggc cgaacacgtt gtcgccgacc cacaggccgt ccctgtcgtc gaacgtgcaa
       61 tcgccgagtg cattcggctt ggtgaccgca atcgagcggc tgcgatcgat cgcgtagtgc
      121 ccaccttgct cggtgaggca gtagacgcgc ccttcgccct tgctgcacgc aaaccccgca
      181 cagccctgcg gcgtggtggc gagcgcgagc ccttcgtagt tgatcgcgca cttcttcttc
      241 gtcgggcacg tcgtgccgtc ggcctgagtg ccgatcgcat acggtccctc gacgaggtcg
      301 aagccgctgc ctttgcgctg ccacacccgc atccagccgt ccgaggtgag gccgaacagt
      361 ttgccgtcgt gcgcagcgaa gccttcgatg tcgtcgctag cgccggtgcc gagcggcagc
      421 ttgccttgtt cgcgcgtgac gccggtgtcc ggatcgagca ccgcgtacgc gccgttgttg
      481 ccgctatccg agacgatgac gagcgcgagc ttgccgtcga tcgcgagcca cgcagccgcc
      541 gacgcctcgg gcaatggcgt cgactcggca aacggctgtt gcccgcaatg gccgggatcc
      601 atgtcggaac tggcggtcgc cgccgcggcc gcgatctcgg gtgttggcgc tgctgctgct
      661 gcgggctcgg ccttctgcgc aggcttgtcg ccgcctcgac aggcgatcac cgcgagcatc
      721 actacgcaca gccgcacgcc cgcgctatag cattcccctc gacggaatgc ctgaaccacg
      781 ccgctacctc gtcaaagaga tgttcggccc gacgctgcaa ggcgagggcg cgcacgcggg
      841 acggccgtgt gtcttcttgc gcttcgcggc ctgcaacctc gattgcaagt ggccctgcga
      901 tactgacttc tcggtcgaag gcgccgtacg tcacacggca gaggagatcg tcgcgaagct
      961 cctcgagcta gacacgaacc gcgcgcgcat ggtggtcgtg accggcggcg agccgacgtt
     1021 gcaatgggat gcgccgctcg ccgaggcatt gcacgtggcg ggctttcgca tccacatgga
     1081 gtcgaacggc acgcggccgc tcgccgcgtc ggtcgattgg ctcaccgtgt cgccgaagcc
     1141 gcagtttcat ccggcatcga tttcgctcgt caccgacctg ccggtcagcg agctcaaggt
     1201 cgtcgtcgac gacacggtcg acgaggccac gctcgaccgc tacgtcgagc gctaccgctg
     1261 cacgcactac ttcgcacagc cgtgtatgaa cgaccagtat cagcaccatc tgcagcgcac
     1321 gctcgcgctc gtcctcaaac ggccgcaatg gagaatgtca gtccagctgc acaagatcat
     1381 cggcgttccg tgatcctggt tgtaccggca acgtaaggtt gcactggctc gggggcggca
     1441 tgacgcgttt tgccgcgctc cacaagatcc tcggcgtgcc gtaagctcgg gcgtgctcgc
     1501 gctcgaccag gcctcgcagt tcgctcagct ggcgctggcg tgcgtgcggc gcgagctgcc
     1561 tcacaagctc gatcacttgc tgctcgccgg cacgacgcca tcgccgcgcg agctgcatcc
     1621 cgccttctac ggctgcctcg actggcactc ggcggtgcac ggccactgga tgctcgcgcg
     1681 cctgctcgag aaatttccca cgctgcccga agccgcagcg atccgcgcga cgttcgacga
     1741 tacgttgacg gcggaaaatc tcgcgaccga agcggcgtac ttcaccgatc ggccgtcctt
     1801 cgagcgcatg tacggctggg catggttgct gaagctcgcg tgtgatctgc ccgcgccgtg
     1861 gcgcgcgcct ctgcagccgc tcgtcgacgt gatcgtcgag agctaccgcg cgttcttgcc
     1921 gaagcaaacg tatccggttc gcaccggcgt ccactcgaac acggcgttcg ggctcacgtt
     1981 cgcgctcgac tatgcgcgcg cggtttccga tcaggcactc gagcagctga tcgtcgatcg
     2041 gtcggtcgcg tattacggtg gcgacgtgtc ggcgccgagc gcctgggaac cgagcggcga
     2101 agatttcttg tcgccatcgc tcgtggaggc ggatctcatg cgccgcgtgg tgccggactt
     2161 tccgacctgg ttcgtgcgct tcatcccgac gttgtcaccg agccttcgtt cacccgccgt
     2221 cgtcagcgat cgcagcgatc cgaagctcgc gcacctcgat ggcctcaacc tgtcgcgggc
     2281 gtggtgcttg cgcaatatcg ccgaggcgct gccaccgacc ccgttgcgcg acgagctcga
     2341 ggtcgcggct gcggcgcacg cgcgtgatgg gctgaagcat gtggccacgg gccagtacat
     2401 gggcgagcac tggctcgcgt cattcgcggt ctacctcgag gtgccggcat gacgttgctc
     2461 gtcgcgcgca tggtggaaga gcggtttcat cgcgacaccg aggggcggcc gattcgcgag
     2521 ggacgaacgc cacctgagtt tctgcaggcc ggtgagatcg agttccgcac gtgtccgtac
     2581 tcgggctcgc ggtttcagca caagaacccg atgaacgtct cggcgttgcg gcagatgggc
     2641 gcgcactggg acgagctgtg cgacgcgctc gcgttcttgc gcgccgcgca cgacgaggtg
     2701 ctcggcgtga cgcagccgca gctgatggat gtgtggcgtg cgagtcagct cggctcggca
     2761 ttgccatggt tcttcttgtt ccgcgatgcg gaaattccgc cgtacgccgc tgcactcgcg
     2821 aaggcgacgc tcggcatggg catctgggcg cagcgcgagc tgttgcagac gatcgcgaca
     2881 gcacggccag cgccggcgct gacacccgag tcgatcctcg cgatggccga ggcgaacggc
     2941 acgctcgtcg gcgagaccga ggtgtgctcg ggcggcgaca agatgttgct caagttcttc
     3001 gaggtactcg tcggcccaag ggcgccagga cgattcgctg cgccgcgcga cgaggtgctt
     3061 cgcttcggcg cgcactacac gaacttcaag ctgtacatct ggctctacta cctcgcgcgt
     3121 cgcttcctgc tcgccgacgc cgtcgcagcg cacggctcga gtcccgaggc ggccgcactg
     3181 ctcgatgcgt ccgtcgagcc gagcgacttc ttccgcatcg agcccgcgaa cctcgccgcc
     3241 gtgatcccgg agcagcgcat gggatggttc cgctcgctcg ccgatctgat cgtgcccttc
     3301 gcgccggcgc ggaccgatct ggcgttgcgc gacctcgcgt ttcagctcgc cgcagcgatg
     3361 gggaagggcg ccggcccgac cgagacgttc gccgtgctcg acgagctcta cggcaaggtc
     3421 atcgcaaacg tcgaggcggg ctttcgcggc ggcgttggta ccagcactgc cgacatcgat
     3481 gtccctgcag atattcggga tcgcctcgtc gcctcgtcgc cgcggcaatt ctttgcgtcg
     3541 ctgccgagcg caccgccgcc cgaccgctag tctctgtatt cgccgcgcag catcgacagc
     3601 gcgatcacgc acacgatctg caaaccggga atgatcatcg cgtgccacag gccgacgccg
     3661 aggatgtggt tgaccttcgg cgcgccaaag aaggtcaccg cgccgaacac ggagacgacc
     3721 gcgatcaaga acgaccacgc cgcgcgcttg tgctggagcg acatccacgt gagccacggc
     3781 aacgcgcagc cgacgacgag catggtcatg cccatcaccg gtggcatgcc cttggagaga
     3841 gccgagatac cgccgacgat cgcggcgaga cccatcgcga tcgcgatgcc gtgaccgatg
     3901 aggcgcggcg cgagcgcggc ggcgtacgcc atgccggcga caatcagcga cagctgcaga
     3961 aacgcgaacc gcacgcttcc ggtgtccgcg gcctcgagcg gcttgtcctt gaagtagaaa
     4021 tacgacgcga cccagaacag cgtgttgaac gtgaggatgc caccggcgat cacgccggcc
     4081 atctgcatgc gctgcttgaa cgtggtgcgg gtgcgggcga cttcagccat cgcggtgtgc
     4141 ctagcttact tcttgcgctt gaacagtgag ccccaaaaac ccttgggctt gtcgccgtta
     4201 ttcccggccc cctcgatgcg caccgagggc tcttcttctt tgctgacttg gacgcttggc
     4261 atggtcgccg tcggcgtctc tggctcggat tgccccgtgg gggtctttgg catgtaaccg
     4321 gggatgctcg cggcgaaact caggccgggc tcggcctcga gcgccatctg cgtctcctcg
     4381 agctccttct gatactcctt gcggaacagc tggcggatga actgccgcag ctccttggga
     4441 tcgaagcgat agcccgcgat cagcgcgtcg agatcggcgg cgagctgcgc ggccgattgg
     4501 tagcgatcgg cgacgtcacg ggcgagcgcc ttgaacacga tcgcatcgag ctcgggggtg
     4561 acgcggcgat tgaagttgga cggtggcggc acctcggctt tgcgcacctt ctccatcagc
     4621 gcgaattcgg tgtcgccgcg gaacagcttc tcggtcgtca gcatctcgtg gaggatgatg
     4681 ccggcggaga agacgtcggt acgcgcgtcg agcggcatgc cgcgaatctg ctcgggcgac
     4741 atgtaactga acttgccctt gaggatgccg atctcgctcg tgaacttcat cagcgcctgc
     4801 gcgatgccga agtcgagcag cttgacgtca ccgtcgtagc tcatgcgcac gttcgacggc
     4861 gagacgtcgc ggttgacgat gttgagcggg cggccgtcgg ggccctgcag cgtgtgcgcg
     4921 aagtgcaaac cttccgcgat gcgcgcagcg atatagaccg cgtgcggaac cggaatgcgt
     4981 tgctgggtct cctgacagcg acgcagcacc tgcgtgagat cgcgaccgcc gatgtattcc
     5041 atcgcgatgt agtggcggcc gtcgtactgc ccgatctcga gcgtctcgac gatgcagcgg
     5101 ttcttcagca tcatcgcgag ctttccttcg cgatggaaca tgtcgatgaa gcgcgcctcg
     5161 cgtgcgagct gcggccgcat caccttcacg gcgaacagat tcttgtcgcc gttcgcgatg
     5221 cgtgcgcgat agacctcggc catgccgccg cgcgcgatca gtccaagaag gtaatacttg
     5281 ccgaacggca ccggatcatt cggcgcaccc ggcgacaact tcccttcctg tgacggccgc
     5341 tcggagacca cggcgcgggc accttaccac tgcacgacga gcttttgtcc cggctcgatc
     5401 tgtacggatc gctcttctgt cctaaccccg gaatcgtcct tcaagaccat tcggagcttg
     5461 tgaccatcgg acggaaccgg gacgcccacc tcgagcgagg tgatcccgag gtcgttcatg
     5521 acgcctggcg agctcgccat ttcgagggcg attcggccgt agggcttctc cagcttgttg
     5581 acgacgatcg cccagcggaa cgtcatcgtg gatggcttgc caggctcgac ggtcgtgtac
     5641 tcgaacatcg ccatctcgta cttgggattg aagcacagcc ccttgtacgt accaggcgca
     5701 acgagcaggc ccttctccgg caacttgccc gagatgcgct ggcggttcac ccacatttcg
     5761 ccgcagggca tgctcgccga gacgagcagg gtgccgagca catccttctc gcgcgcgagc
     5821 tcgctgcgca cgacatcggc gttcgggcca aacggtcgct gcaccagata gcggcgatag
     5881 aagtccttct tgcgctcgcc ttcgccgagc ttgtcgtagg tcgaaccaag ctcgcgcaag
     5941 aagccgggca gctcgggcgc gagcgcgtag cccatatgga acagcacgag cgcgtcggcg
     6001 tagcggccgg ccttgaactc ggatcggccc tgcttgaacg cctggttcgc ttccttcgac
     6061 atctcggtca gcgcgagctt gtccgcgaat ggatcggggg ttccggcgag tcgcgtcgct
     6121 tcggcggttg cctcggcttt ggccttgggg tccttcacag ccgccgcgta attgctccac
     6181 gcctcggcgg cctcttggtt ttgaccggcg agtgtccaca gcgtcgcgag ctgctttgcc
     6241 tcggggctgg ccggaccgcg cgcgaccgcg agcgcttgat acagcgggat tgccacggcc
     6301 cacttgcggt ccttcaccgc tttttcagcg cctttgacca ggtgttgcga cagcgccgcg
     6361 cgcgacttct tggtcgtgac ggtcgagtac agctctgcct gatcaggcgg tttgacgtcg
     6421 gttttatcgg gcggtttgtc ctcgccatcg gcgcgggcaa ccggcgcaaa caacaacagc
     6481 gcgacgacag gcgtcgcacg aaggacccaa ttcacaaaac aagcgtaacc ggtcgcgatc
     6541 ttggacacga gtcttcgacg aaagatcgtg ctccgagatt cttgcgcgcg acaactctgc
     6601 gcgagtacgc ggccgcccac tttggatccg agcaaccgac ctgcaaagta gccgggcgca
     6661 cgcacattac gggaactgcc aaaaacggtt tatcaacaat agtttgcatt atgccaacgc
     6721 gtggtacgtt ggacgtgttg cacgttgtca tccttgaacg caacaagcag gtggggcgca
     6781 aagtcgctcg gctgttcctt tcggtgggtg cgacgggtgt ggtcgtggaa gagcccaagc
     6841 atgtcgcccc ggtgatcgaa gacgccgacg tcctatgcgc cgacgcgttt gacggtgatt
     6901 ttgtcgccga ggcagtgcgg tctcgcccgc ggctgcgcgg cgtgttgtgg accgccgagc
     6961 cgttgcgacg cgcgctcaag ttcctcgtcg agacgagggc gatcgacaac gtgctcggtc
     7021 gcaaggactt cgacagcgcg ccgcgctcgt gggaagtcct catggtcgcg cgccggcttg
     7081 gcggtgctcc gccggctcca ctcagtgcgt atctcgattg gggcttctcg cacctcgatc
     7141 tcgacgtccg cacgaccgcg gatcgcgatc tctcggtcgt gaagattcag gattttgtcg
     7201 cgggcctgca gctgccgaag cgggtcggcg agatgttcgg cgagctcgcg cacgagctgc
     7261 tcatgaatgc gatgtacgat gctccggttg acgcgcaggg gaaggctaaa tacgccgctg
     7321 atcgcaaggc cgacatccgg ctcggcgaca acgaacggcc gaccgtgcgc gtcgcgaccg
     7381 atggaactcg actcgttttg caagttcgcg atccgttcgg tcggctcgag cgcaggcacg
     7441 tcttcgacgg gctcgcgcgc gggttggccg gtggtgaaat ggatcagagt cacggcgggg
     7501 ccggcctggg aatgatggtc tgtcacaact cgtcgtcggc gatgttcttc gacgtcgtgc
     7561 gtgggcggtc gacggaagtc accgctctct tcgagctcga tatgaatctt cgcgagctcc
     7621 gcacgcaggc gaagtcgctg cacttctgga gcgcatgatg gaagccgtac gtaaatcaca
     7681 accagcgatg gtatcgcccg gtcgcaattc gggcgcccct ctcggagctc cacaagaacc
     7741 ggcatcggcc gcgaagctga cgatcgagaa gttcgccgac ggcgcgatct cgtgcctgaa
     7801 gttcagcggc acgatcgacg aatcgttcga gggcaagaag gtcgcgcggt cggtgcaggg
     7861 cgatacgctc gtgctcgacc tcggcggcgt gaagaagatc tcgtcgttcg gtattcgtg
//
LOCUS       DASUFX010000273         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_737621,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000273 DASUFX010000000
VERSION     DASUFX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737621"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFV99_10830"
     CDS             <1..408
                     /locus_tag="VFV99_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX5059846.1"
                     /translation="GGRLGHAIEPALAPLGFDWKIGVGIIGAFAAREVFVSTLGIVNA
                     VGDDADERSEPLRQALREAKKPDGTKAYTPLVALSLMVFFALAMQCMSTLAVVRRETQ
                     SYRWPLFLFGYMTVLAWVASFLVFQGGRLLGFG"
     gene            477..866
                     /locus_tag="VFV99_10835"
     CDS             477..866
                     /locus_tag="VFV99_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5059847.1"
                     /translation="MQYLLLIYDNEKLWGDMSKEESQKLHGEYTVFTNDIKASGHFVA
                     GDALQPVATATTVRVRNGKTQTTDGPFAETKEQLGGYYLINAKDLDEACKIAERIPSA
                     RIGSIEVRPVMVFNMGNAKDQYMAAKK"
     gene            complement(869..2983)
                     /locus_tag="VFV99_10840"
     CDS             complement(869..2983)
                     /locus_tag="VFV99_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059848.1"
                     /translation="MRALSYIVVVVLAACGQKQSTAPCPDTKPTREAAGAKPAVSAED
                     TPIAANDVAAAKTACVKKHGEANRAAVEKGVDQVASLWRVADGDLGAFCEEQFVADPK
                     LRDQLFERLQEMNEQMKGHFLELGRTARWNTEVDTGPMAAVEPLLAAYDPTAHTIEDM
                     FKTKVAFAALLNFPLTKLADRLKDGESYSRRQWAEVRLTGKYDTRVPGALLQAATAAE
                     AAADQYIAGYYLWMHHVLAEDGKRLFPAKKHLITHWNLRDELKANYADADGLAKQRVI
                     IKVMERIVTQQIPAAVIDNPNLDWNPFTNKVTVAEAGTVEAEAPAGKTKEPSDTREAD
                     TRFEQVRRHLAAGRAIDPYVPVAPTVIERAFDAAEMPEDRVRKLLVEILESPLAADAA
                     KEMQQKLGRKLEPSDIWYEFGQDTGPETELDKITTKKYPTPQAFEKDIPRILRDLGFT
                     PERASYVASHITVDSSRGAGHAMGAERRGDKAHLRTRVEPGGMTYKGYNIAVHELGHN
                     VEQTFSLYDIDYTLLSGVPNTAFTEALAFLFQARDLELLGKPKANGEADRLRVLDAFW
                     NTREIAGSALVELDVWHWLYDHPNATAAEIREATVKTAQSVWNKYYEPTLGGHDSALL
                     GIYSHTITSPLYLFNYVLGHLIAFQVEEHVAGKDKATFAKEFERVCRLGMILPDLWMK
                     QATGNPVTAQPLLDATARALKK"
     gene            3019..>3356
                     /locus_tag="VFV99_10845"
     CDS             3019..>3356
                     /locus_tag="VFV99_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5059849.1"
                     /translation="MQTAAAIADVFRVERARVLATVIRVCNGDFELAEEAVQEAFEAA
                     LARWPTEGIPDEPRGWLVSVARNKAVDTIRRRIKLREIVASQDVAPESQVGAAPETIA
                     VPDDRLRLIFT"
BASE COUNT          536 a         1069 c         1123 g          628 t
ORIGIN      
        1 ggcggccgac tcggccatgc gatcgagccc gcgctcgcgc cgctcggttt tgactggaag
       61 atcggcgtcg gcatcatcgg cgcgtttgcc gcgcgcgagg tgttcgtgtc gacgctcggc
      121 atcgtcaatg cggtcggcga cgacgcggac gaacggtccg agccgctgcg ccaggcgctg
      181 cgcgaggcga agaagcccga cggcaccaag gcatatacgc cgctcgtcgc gctgtcgctc
      241 atggtgttct tcgcgcttgc gatgcagtgc atgagcacgc tcgcggtcgt ccggcgcgaa
      301 acccagagtt accgctggcc gctcttcctg ttcggctaca tgaccgtcct cgcctgggtc
      361 gcgagcttcc tggttttcca gggaggtcgc ttacttggct tcggctgatc tttttacaag
      421 gtcgttgtcg atccgcctcg cgcccattcg acccatgggc gtaaggagag atgaccatgc
      481 aatacctgct gctgatctac gacaacgaga aactgtgggg cgacatgtcg aaggaggagt
      541 cgcagaagct ccacggcgag tacacggtgt tcacgaacga catcaaggcg agcggacatt
      601 tcgtcgccgg tgatgcgctc cagccggtag cgaccgcgac gacggtgcgc gtgcgcaacg
      661 gcaagacgca gacgaccgat ggaccgttcg ccgagaccaa ggaacagctc ggcggctact
      721 acctgatcaa cgcgaaggat ctcgacgagg catgcaagat cgccgagcga atcccgagcg
      781 cacgcattgg ctcgatcgag gttcgcccgg tcatggtctt caacatgggc aacgccaaag
      841 accagtacat ggccgcgaag aagtagcgct acttcttcag cgcgcgggct gtcgcgtcga
      901 gcagcggctg cgcggtgacc ggattgccgg tcgcctgctt catccagagg tctggcagga
      961 tcatgccgag ccggcagacg cgctcgaact ccttcgcgaa cgtcgccttg tctttgccgg
     1021 caacgtgctc ttcgacctgg aacgcgatca agtgaccgag cacgtagttg aagagataca
     1081 gcggcgacgt gatcgtgtgg ctgtagatac cgagcagcgc ggagtcgtga ccgccgagcg
     1141 tgggctcgta gtacttgttc cacacgctct gcgcggtctt caccgtcgct tcgcgaatct
     1201 cggccgcagt cgcgttcgga tgatcgtaga gccagtgcca gacgtcgagc tcgacgagcg
     1261 ccgagcctgc gatctcgcgc gtgttccaga acgcgtcgag cacgcgcagg cgatcggcct
     1321 cgccgttcgc ctttggcttg ccgagcagct cgagatcgcg cgcctggaac aagaacgcga
     1381 gcgcttcggt gaacgccgtg ttcggcacgc ccgacagcag cgtgtaatcg atgtcgtaga
     1441 gcgagaacgt ctgctcgacg ttgtggccga gctcgtggac cgcgatgttg tagcccttgt
     1501 acgtcatgcc gcccggctcg acgcgcgtgc gcaggtgcgc cttgtcaccg cggcgctcgg
     1561 cgcccattgc gtgaccggca ccgcgcgacg aatcgaccgt gatgtgcgag gcgacgtagc
     1621 tcgcgcgctc cggcgtgaag ccgaggtcgc gcaggatgcg cgggatgtct ttctcgaacg
     1681 cctgcggcgt cgggtacttc ttcgtcgtga tcttgtcgag ctcggtctcg ggaccggtgt
     1741 cctggccgaa ctcgtaccag atgtcgctcg gctcgagctt gcggccgagc ttctgctgca
     1801 tctccttcgc ggcgtcggcg gcgagcggcg actcgaggat ctcgacgagc agcttgcgta
     1861 cgcggtcctc cggcatctcg gcggcgtcga acgcgcgctc gatcacggtc ggcgcgaccg
     1921 gcacgtacgg atcgatcgcg cggccggccg cgaggtgacg gcgcacttgc tcgaaccgcg
     1981 tgtcggcctc gcgtgtgtcg gacggctcct tcgtcttgcc cgcaggcgcc tcggcctcga
     2041 cggtgcctgc ctcggcgacc gtgaccttgt tcgtgaacgg attccaatcg aggttcgggt
     2101 tgtcgatcac ggccgcggga atctgctgag tgacgatgcg ctccatcacc ttgatgatca
     2161 cgcgctgctt cgcgagaccg tcggcatcgg cgtagttcgc cttgagctcg tcgcggaggt
     2221 tccagtgcgt gatgaggtgc ttcttcgccg ggaacaagcg cttgccgtcc tcggcgagca
     2281 cgtgatgcat ccagaggtaa tagccggcga tgtactgatc cgcggcggct tcggcggcag
     2341 tcgctgcttg cagcagcgcg ccgggcacgc gcgtgtcgta cttgccggtg aggcgaacct
     2401 cggcccactg gcggcgcgag tagctctcgc cgtccttgag gcgatcggcg agcttggtca
     2461 gcggaaagtt caacagcgcg gcgaacgcga ccttggtctt gaacatgtcc tcgatcgtgt
     2521 gcgcggtcgg atcgtaggcg gcgagcagcg gctcgaccgc ggccatcggg cctgtatcga
     2581 cctcggtgtt ccagcgcgcc gtgcggccga gctcgaggaa gtgacctttc atctgctcgt
     2641 tcatctcttg cagacgctcg aacagctgat cgcgcagctt cggatcggcg acgaactgct
     2701 cctcgcagaa cgctccgaga tcgccgtcgg ccacgcgcca cagcgacgcg acctggtcga
     2761 cgcccttctc gaccgcggca cggttcgctt cgccgtgctt cttcacacac gcggtcttcg
     2821 cggccgcgac gtcgttcgcc gcgatcggcg tgtcttcggc gctgaccgcg ggctttgctc
     2881 ccgcagcttc gcgcgtgggc ttggtgtcgg ggcacggcgc ggtcgattgc ttctgaccgc
     2941 acgctgcaag aacaacgacg acgatgtaac tgagggcgcg catgaccgag cgtctgtagc
     3001 acagtaagct ggccacacgt gcagacagct gctgccatcg ccgatgtgtt tcgcgtcgag
     3061 cgggcgcgcg tgctcgcgac cgtgatccgc gtatgtaacg gcgacttcga gctcgccgaa
     3121 gaggcagtcc aagaggcgtt cgaggccgcg ctcgcgcgct ggccaaccga aggaattccc
     3181 gacgagccgc gtggttggct cgtgtcggtc gcgcgcaaca aggccgtcga tacgatccgc
     3241 cgccgcatca agttgcggga gatcgtggcg tcgcaggatg tcgcaccgga gtcgcaagtg
     3301 ggcgccgcac cagaaacgat cgcggtcccc gatgatcgat tgcggctgat cttcac
//
LOCUS       DASUFX010000274         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_43090,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000274 DASUFX010000000
VERSION     DASUFX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43090"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFV99_10850"
     CDS             <1..207
                     /locus_tag="VFV99_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059850.1"
                     /translation="KSSETMKEIEFAQRNHSAFQYWDVTDIGGEIYTLRNEGSGKFMS
                     FGFGSHVKQSGDQDQHTHWRLLPV"
     gene            253..972
                     /locus_tag="VFV99_10855"
     CDS             253..972
                     /locus_tag="VFV99_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5059851.1"
                     /translation="MTWRVPPYDRALDELVTYVRATYQPLGIVVAGSIVRGEAGPTSD
                     FDVFIVHDASWRLREQRVFAGVPAELFVNPAARVRRYFENEHARGRPCTAHMFATGEA
                     LEPVHSTIAELIAEARTWLAKPLEVTEAALVQRRYGAVDCLDDARDVIDRDPAAAALL
                     LAEVVREIVAYAFWQRRAFEPRRKDMVARLEAIDPEAASLLRTWSAASGRQALAIVET
                     LARHVLGVDAFFEWTSERDEE"
     gene            complement(1027..1806)
                     /locus_tag="VFV99_10860"
     CDS             complement(1027..1806)
                     /locus_tag="VFV99_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059852.1"
                     /translation="MAFRCALLVLLLDACGRFGFSGQSHEDATPGGGDDASSDSAVID
                     GALTDSVTDTMLVDAPPCTGTTHTITDNFDDNSRNTALWGNAYADSVTSYAETGGHVV
                     ITLGVNIANNWAGYMTTSGYSIAGDRVFVEVPQVSNPTTNTILLLWTSLAKTDGPSIE
                     WEANKLFFRMRINDQIFDRANVTYSATTHRWWQIRERAGTMYWETSPDGVSWTVQHME
                     ATPASTTAYITFAAGTNSANATPGTAIFDNFNGGGAPPSCP"
     gene            complement(1829..2752)
                     /locus_tag="VFV99_10865"
     CDS             complement(1829..2752)
                     /locus_tag="VFV99_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059853.1"
                     /translation="MKHLAVVALFLACQPPGGTTATHPVEPAKQVTLLDLIGDWRWVY
                     RAEEEATARVEEEQWQLRADPAAPTRLIGRYLRTVEVRSTDRVPFQCNQRLYYRQRAM
                     FDVIADLQGTELIVKETAYKAEPSPCDHGFRHMSEYRMQPHGNRLTLRWNKGSQTLWQ
                     VDSVSTPLRDAPWTEKPEPTGPWRWQATSFDDDGNVRDEAEWWEITRRTDTRLDMTYR
                     RRVTVRSPDGKPIACANAPSWTFDDAYVLSGQKEEEHWHFYELAVEPGDHPCLRATPK
                     RALDEATAEQIGDFLVLEWRGKRRQILYRSE"
     gene            complement(2763..3038)
                     /locus_tag="VFV99_10870"
     CDS             complement(2763..3038)
                     /locus_tag="VFV99_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059854.1"
                     /translation="MRFEWMECTPGKKNGSKLRADVAAAELANRAGTMYRLGFPEKEA
                     VARLCARVAWEYDPSAKAGHHRRPEALSDKAIAKIVADTYARRPGGW"
     gene            3059..4270
                     /locus_tag="VFV99_10875"
     CDS             3059..4270
                     /locus_tag="VFV99_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059855.1"
                     /translation="MRQLSVAGAIAIALAGSAHAEGVGVIAAGGDRGDASKAMAGAIA
                     DARPARIVDDAVGEARAAVVAGAVPVATLERFRHVREQIDEAWRAFLRVQVDFAASRL
                     AAARTAAESLVSLPGGASVYADASLRLGAVLGYLGRAADSQAILALALALDPERPITI
                     AEFSPDVVSAVDAVRAQTRPTRAVRITSEPAGAAISVDGKELGRAPLHADLAVGQHVV
                     VGRAAQYQARAMAVAIGDGANELSVELERDIGWTRLAQGATIGASDADAQELVDAAVR
                     YADLDEVVLVAAAEQRGGPTLLVQRCAGLPARCTAVIEVGYAEHSGIGAAAREAWQAA
                     RVADLRYPPSVFGDPRATGKKGDGRCKLCRSPILWGGVGVAAVVATVAIIAIVSSSRP
                     PPVVGVDPTQF"
     gene            complement(4267..6063)
                     /locus_tag="VFV99_10880"
     CDS             complement(4267..6063)
                     /locus_tag="VFV99_10880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059856.1"
                     /translation="MTELPTTFGKYYLTEKLATGGMAEIYLAKIIGPGGFEKPLVIKQ
                     IHPKLSGNRHFVDLFVAEAKTLVTLAHGNIVPVYELGVVDDTYFIAMEYIDGPTLYRL
                     TETMARREGHMEPALAAWITARILDGLDYAHRKGDGVIHRDLSPRNVMLSRDGEVKLV
                     DFGIAVTLGDGTEGEDSRSAPTGSFPYMSPEQVRRESLSGQTDLFSVGVLAWEMLAGR
                     RLFAKGDADATLKAVLEEPIPPPSSVRPEVPAKLDEVVMRALERNTNARWPTAADMLA
                     QLNKHLYGLDETPGARDVSALVARYCPPETRRLPTNLEAVAHDVVSPEPLADGRQPTA
                     PIEPAPGGPRTAVIPREAAARGKPKRHQTFATNVNMQEMLDRATPLLPFDAIDDTPTK
                     DDSPQAIKEPKTLQDAQPFGAEPEPNVTARIARPPSRALLVLVGIGGLALAAGAVYIF
                     FANRERVLHPRDASVPVMYFDAPAVEQTVDAIVVEPPIDAGVIDAGAIDAGREPPRDA
                     GTRRSDATRPPSDASTTTAATGTLEVGAIPWGEIYVDGKHAGRTPKKVSVSAGHHTVE
                     IVFPAESPPRKQTFAVDLASGETKPLQADFTN"
     gene            6099..6653
                     /locus_tag="VFV99_10885"
     CDS             6099..6653
                     /locus_tag="VFV99_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5059857.1"
                     /translation="MRAWCLVLVSTAGCAQLFGIEQTTSSEVRTDAAVDARRDSGIDA
                     RTCSAGDAHVTDPMTGACYMFFVGPKTRDEARATCNTEGALLASVESANENQLITSLL
                     GMNLAFLGGTDEVTEGTFKWEDGTAVVLTNFATGEPNNGTGLFEEDCIVIHGDLAGKW
                     DDRPCGAEAGATVPGAYAFVCERD"
     gene            6705..>8113
                     /locus_tag="VFV99_10890"
     CDS             6705..>8113
                     /locus_tag="VFV99_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059858.1"
                     /translation="MISRAGLCALVLAACGDPAAHVQLTPVNPCGMVTETALRVIGYT
                     AGGELRRTVPPNEIDAFPADTEQLGVEVVGAGGGLIAIGKTAPLAFDALTDGSDIPIV
                     MAPPSGFCPVGPMTEPRTAPLVARAGQGVLIVGGLAPNGDRLSTAEYYDPATTTFTPV
                     DVPPSLVDPDNGLAGAVLSELPDGRVAITGTASHALAYFDADDRRLTMPNLFDHRAFH
                     GAFATDADHLLIIGGCADVAAGSCMGPTLRTGFVYDLRDLAKRDRAPQLGDTAQRFGA
                     HVLELGIQRDGVRRFLLADGFGDAAVADRFALTDATTELVTGMHAQTTLLDGGAVLTA
                     FDPDTAAQTGAASVLVPDGGLAAIALAPRFAGTRVVTLEDGSVIAIGGDANVARYLPT
                     TNTWAVAPPAGTGPGAIVAPVLMRLGDGSVLVLGGRTATAEAWIYRPALVGPASGSVV
                     ALPDGSTEGVLTPTDPTLL"
BASE COUNT         1218 a         2658 c         2841 g         1396 t
ORIGIN      
        1 aagagcagcg agacgatgaa ggagatcgag ttcgcgcagc gaaaccacag cgcgtttcag
       61 tactgggatg tgacggacat cggtggcgag atctacacgc tgcgcaacga aggcagcggc
      121 aagttcatgt cgttcggctt cggttcgcac gtgaagcagt cgggcgatca ggatcagcac
      181 acgcactggc gtctgctccc ggtttgagcg agtagttgtc attcgcgcga gcgcgcttgc
      241 taggctgcag caatgacgtg gcgtgtgccg ccctacgacc gcgccctcga tgagctcgtg
      301 acgtacgtac gcgcgacgta ccagccgctt ggaatcgtgg tcgcaggcag tatcgtgcgc
      361 ggcgaagccg gcccgacgag cgacttcgat gtgttcatcg tgcacgacgc gtcgtggcgg
      421 ttgcgcgagc agcgcgtgtt cgcgggcgtg cccgcagagc tgttcgtgaa tccggccgcg
      481 cgcgtacggc gctacttcga gaacgagcac gcacgcggcc ggccgtgcac ggcgcacatg
      541 ttcgcgaccg gcgaagcact cgagccggtg cattcgacca ttgccgagct gatcgccgaa
      601 gcgcgaacgt ggctggccaa gccgctcgag gttaccgagg ctgcgctcgt gcaacgccgt
      661 tacggtgcgg tggattgcct cgatgatgcg cgtgacgtca tcgatcgcga tccggcagcg
      721 gctgcgctac tcctcgccga ggtcgtgcgc gagatcgtcg cgtacgcgtt ctggcagcgg
      781 cgcgcattcg agccgcggcg taaggacatg gtcgcgcggc tcgaagcgat cgatcccgag
      841 gccgcgtcgc tcctgcgcac gtggtctgcg gcgagcggcc gccaggcgct cgcaattgtc
      901 gagacgctcg cgcgccacgt gctcggcgtc gacgcgttct tcgagtggac ctcggagcgc
      961 gacgaagagt aggactagcg ccaaggctag caagcaacga tgcgtcgcga tgtggcgatt
     1021 gtcgcgctac gggcagctcg ggggtgcgcc gccgccgttg aagttgtcga agatcgccgt
     1081 gccaggcgtg gcgttcgcgc tgttcgtacc ggccgcgaac gtgatgtacg cggtcgtgct
     1141 cgctggcgtc gcctccatgt gctgcaccgt ccagctcacg ccatccggcg acgtttccca
     1201 gtacatcgtg ccggcgcgtt cgcggatctg ccaccagcga tgcgtcgttg cgctgtacgt
     1261 gacgttcgcg cggtcgaaga tctggtcgtt tatgcgcatg cggaagaaca gcttgttcgc
     1321 ttcccactcg atcgatggac cgtcggtctt cgcgagtgac gtccagagca gcaggatcgt
     1381 gttcgtggtc gggttactga cctgcggcac ctcgacgaac acgcggtcac ctgcgatcga
     1441 gtatcccgac gtcgtcatgt agccggccca gttgttcgcg atgttcacgc caagcgtgat
     1501 cacgacgtga ccaccggtct cggcgtagct cgtcacgctg tcggcatagg cgttgcccca
     1561 cagcgcggtg ttgcgtgagt tgtcgtcgaa gttatcggtg atggtgtgcg tggtgccggt
     1621 gcatggcggc gcatcgacga gcatcgtgtc ggtgacgctg tcggtgagtg caccatcgat
     1681 caccgcgctg tcgctgctcg cgtcgtcacc gccgcccggc gtggcgtcct cgtgcgactg
     1741 accgctgaag ccgaagcgcc cgcaagcgtc gagcagcagc acgagcagag cgcaccgaaa
     1801 cgccaccgca cgatcctacc gccggcgctt actccgagcg atagagaatc tggcggcgct
     1861 tgccgcgcca ctcgaggacg aggaagtcgc cgatttgctc ggcagttgcc tcgtcgagcg
     1921 cgcgcttcgg ggtcgcgcgc aggcagggat ggtcgccggg ctcgacggcc agctcgtaga
     1981 agtgccagtg ctcttcttcc ttttgcccgg atagcacgta ggcatcgtcg aacgtccagc
     2041 tcggtgcgtt cgcgcacgcg atcggcttgc cgtcggggct gcgcaccgtg acgcggcggc
     2101 ggtacgtcat gtcgaggcgt gtatcggtac gccgcgtgat ctcccaccac tcggcttcgt
     2161 cgcgcacgtt gccatcgtcg tcgaacgacg tcgcttgcca gcgccacggc ccggtcggct
     2221 ccggtttttc ggtccacggt gcatcgcgca gtggtgtcga aacagagtcg acttgccaca
     2281 gcgtctgcga gcctttgttc cagcgcagcg tgagccggtt gccgtgcggc tgcatgcggt
     2341 actcgctcat gtggcgaaag ccgtggtcgc acggcgacgg ctcggccttg taagcggtct
     2401 ctttgacgat cagctcggtg ccttggaggt cggcgatgac gtcgaacatc gcgcgctggc
     2461 gatagtagag gcgctggttg cattgaaatg gcacgcgatc ggtcgagcgc acttcgaccg
     2521 tgcgcaggta acgcccgatg aggcgcgtgg gggcggcggg atcggcacgc agctgccact
     2581 gctcctcttc gacccgcgca gtcgcttctt cttccgcgcg gtagacccag cgccagtcac
     2641 cgataaggtc gagcagcgtg acttgcttgg ccggctcgac cggatgcgta gcggtcgttc
     2701 cacccggcgg ctggcaggcg agaaacagag cgacgaccgc gaggtgcttc acgacgtggc
     2761 gatcaccaac cgccggggcg gcgagcgtac gtgtcggcga cgatcttcgc gatcgccttg
     2821 tcggagagcg cctcggggcg acggtggtgg ccggctttgg cgctcgggtc gtattcccag
     2881 gccacgcgag cgcacaggcg ggcgactgct tccttctcgg ggaagccgag gcgatacatc
     2941 gtgccggccc ggttggcgag ctcggccgcg gcaacgtcgg cgcgcagctt cgagccattc
     3001 ttcttgcccg gcgtgcactc catccattcg aaacgcatgc gccgagtata ctcgggcggt
     3061 gaggcaactg tcggtcgcgg gtgcgatcgc gatcgcgcta gccggatcgg cgcacgccga
     3121 gggcgtgggc gtgattgcgg ccggcggcga ccgcggcgac gcgagcaagg cgatggcggg
     3181 agcgatcgcg gacgcacgtc ctgcgcgaat cgtcgacgat gcggtcggcg aggcgagggc
     3241 ggcggtggtt gccggtgccg tgccggtcgc gacgctcgag cggtttcgcc acgttcgcga
     3301 gcagatcgat gaagcgtggc gcgcgttctt gcgcgtgcag gtcgatttcg cggcgtcgcg
     3361 cctggcggcc gcgcggacgg cggcggaaag cctggtctca ctgcccggcg gtgcgagcgt
     3421 ctacgcggat gcgtcgttgc ggctcggcgc cgtcctcggc tatctcgggc gcgcggcgga
     3481 ctcgcaggca atcttggcgc tcgcgctcgc actcgatccc gagcgcccga tcacgatcgc
     3541 ggagttctcg cccgacgtcg tgagtgccgt cgatgcggta cgtgcgcaga cgcggccgac
     3601 gcgcgcggtg aggatcacga gcgagcccgc gggtgcggcg atcagcgtcg acggcaagga
     3661 gctcggccgc gcaccgttgc atgccgatct cgcagtgggc cagcacgtcg tcgtcgggcg
     3721 cgccgcgcag tatcaggcaa gggcgatggc agtcgcgatc ggcgacggtg cgaacgagct
     3781 gtctgtcgag ctcgagcgcg acatcggctg gacgcggctc gcccaaggcg caacgatcgg
     3841 cgcgagcgac gccgatgcgc aggagctcgt cgatgcagcg gtccgctacg cggatctcga
     3901 cgaggtcgtg ctcgtcgctg cggccgagca acgcggtggg cccacgctgc tcgtgcagcg
     3961 ctgcgcgggc ttgccggcac gctgcaccgc ggtgatcgag gtcggctacg ccgagcacag
     4021 cggcatcggt gcggctgcgc gggaggcatg gcaggcggcg cgggtagcag acctgcggta
     4081 tccgccgagc gtgtttggcg atccacgcgc gaccggcaag aagggcgatg gacggtgcaa
     4141 gctgtgccgc agtccgatcc tgtggggcgg cgttggcgtc gctgcggtcg tcgcaacggt
     4201 cgcgatcatc gcgatcgtat cgtcgtcgcg gccgccgccg gtcgtcggcg tcgatccaac
     4261 ccagttctaa ttcgtgaagt ctgcctgcag cggcttcgtc tcgccgctcg cgagatcgac
     4321 ggcgaatgtt tgcttgcgtg gcgggctctc ggcgggaaac acgatctcga ccgtgtggtg
     4381 gccggccgat acgctcacct tcttcggcgt acggcctgcg tgcttgccgt cgacgtagat
     4441 ctcgccccac ggaatggcgc cgacctcgag cgtcccggtt gcggcggtgg tcgtcgacgc
     4501 gtcgctcggc ggccgtgtcg catcgctgcg gcgcgtaccc gcatcgcgcg gcggctcgcg
     4561 gcccgcatcg atcgcgccgg cgtcgatcac accggcatcg atcggcggct cgacaacgat
     4621 cgcatccaca gtctgctcga cggcgggtgc atcgaagtac atcaccggca cgctcgcgtc
     4681 gcgcggatgc agtacgcgct cgcggttcgc gaagaagatg tagaccgcac cggcggcgag
     4741 cgcgaggcca ccgatgccga cgaggaccaa cagcgcgcga ctcggtggcc gggcgatcct
     4801 cgccgtgaca ttcggctcgg gctcggcgcc gaacggttgc gcgtcctgga gcgtcttcgg
     4861 ctccttgatc gcctgcggac tgtcgtcctt cgtcggcgtg tcgtcgatcg cgtcgaacgg
     4921 caacagcggc gtcgcgcgat cgagcatctc ctgcatgttc acgttcgtcg cgaacgtctg
     4981 gtgtcgcttt ggcttgccac gcgcggcggc ctcgcgcgga atcaccgcgg tacgcgggcc
     5041 acccggcgcg ggctcgatcg gcgcggtcgg ctgccgccca tcggccaacg gctcgggcga
     5101 cacgacgtcg tgcgcgaccg cctcgaggtt cgtcggcaac cgccgcgtct cgggcgggca
     5161 gtaccgcgcg accagcgccg agacatcgcg cgcgccgggc gtctcgtcga ggccgtagag
     5221 gtgcttgttg agctgcgcga gcatgtcggc cgccgtcggc cagcgcgcgt tcgtgttgcg
     5281 ctcgagcgcg cgcatcacga cctcgtcgag cttcgccggt acttccggtc gcaccgacga
     5341 cggcggtgga atcggttcct cgagcaccgc tttgagcgtg gcgtcggcgt cacccttcgc
     5401 gaacaggcga cggccggcga gcatctccca cgcgagcact ccgaccgaga ataagtcagt
     5461 ctggcccgac agcgattcgc ggcgcacttg ctcgggcgac atgtacggga acgagccggt
     5521 cggcgccgac cgcgagtcct cgccttcggt gccatcgccg agcgtgaccg cgatgccgaa
     5581 atcgacgagc ttcacctcgc cgtcgcgcga cagcatcaca ttgcgcggcg acagatcgcg
     5641 gtggatcaca ccatcgcctt tgcgatgcgc gtagtcgaga ccgtcgagaa tgcgcgcggt
     5701 gatccacgcg gcaagcgcgg gctccatgtg gccttcgcgc cgcgccatcg tttcggtgag
     5761 ccggtagagc gtgggaccgt cgatgtactc catcgcaatg aagtacgtgt cgtcgacgac
     5821 gccgagctcg tagaccggaa cgatgttgcc gtgcgccagc gtgacgagcg tcttcgcttc
     5881 ggcaacgaac agatcgacga aatggcggtt ccccgagagc ttcgggtgga tctgcttgat
     5941 gacgagcggc ttctcgaagc cgccgggacc gatgattttg gcgagataaa tctcggccat
     6001 accgccggtc gccagcttct cggtcagata gtacttgccg aacgtcgtcg gcaactccgt
     6061 cattcggctt tgaagagtat cagatacact ctcagtgaat gcgggcgtgg tgcttggtac
     6121 tcgtcagcac ggccggctgc gcgcagctgt tcgggatcga acagacgacc agcagcgagg
     6181 tccgcaccga tgccgcggtc gatgcacgcc gcgatagcgg gatcgacgct cgcacgtgct
     6241 ccgctggcga tgcgcacgtc accgatccta tgacgggcgc ctgctacatg ttctttgtcg
     6301 ggcccaaaac gcgcgacgaa gcgcgcgcga catgcaacac cgagggcgcg ctgctcgcga
     6361 gcgtggagag cgccaacgag aaccagctga tcacctcgct gctcggcatg aacctcgcgt
     6421 tcctcggggg cacggacgaa gtcaccgaag gcacgttcaa gtgggaagac ggaaccgccg
     6481 tcgtgctcac gaacttcgcc acgggcgagc cgaacaacgg tacgggcttg ttcgaagagg
     6541 actgcatcgt catccacggc gatctcgcag gcaaatggga cgatcgcccg tgcggagcgg
     6601 aagctggggc caccgttccc ggcgcctatg cgttcgtctg cgagcgcgac tagaagcgcg
     6661 cgtgaaaccg gcacgtgcac cgcgccggcg gtactattgg cgcggtgatc tctcgggcag
     6721 ggctctgtgc cttggtgctt gctgcgtgcg gcgatccggc agcgcacgtc cagctcacgc
     6781 ccgtgaatcc gtgcggaatg gtgacggaga ccgcgttgcg cgtgatcggc tacaccgccg
     6841 gcggcgagct gcggcgcacc gtgccgccaa acgagatcga cgcgtttccg gccgataccg
     6901 agcagctcgg agtcgaggtt gtgggtgcag gcggtggcct catcgcgatc ggcaagaccg
     6961 cgccactcgc attcgatgcg ctcaccgacg gcagcgacat tccgatcgtg atggcgccgc
     7021 ccagtggctt ctgtccggtt ggcccgatga cggagccgcg caccgcgccg ctcgtcgcgc
     7081 gagcggggca gggtgtgctg atcgtcggtg gcctcgcgcc aaacggtgat cggctatcga
     7141 ccgccgaata ttacgatccg gcgacgacga cgttcacgcc ggtcgacgtg ccgccgagcc
     7201 tcgtcgatcc cgataacggt cttgccggcg ccgtgctcag cgagctgccc gacggacgcg
     7261 tcgcgatcac gggcaccgcg agccacgcgc tcgcgtactt cgacgccgac gatcgccggc
     7321 tcacgatgcc gaacctgttc gatcaccgcg cgtttcacgg ggcattcgcg accgacgctg
     7381 atcacctctt gatcattggc ggctgcgcgg atgtcgccgc cggcagctgc atgggaccga
     7441 cgctgcgcac cggattcgtc tatgacctgc gcgatctcgc aaagcgcgac cgcgcgccgc
     7501 agcttggcga cacggcgcaa cgtttcggtg cacacgtgct cgagctaggc atccagcgcg
     7561 acggcgtgcg gcgcttcttg ctcgcggacg ggtttggtga tgccgccgtc gccgatcgat
     7621 tcgcgctcac cgacgcgacg accgagctcg tcaccgggat gcacgcacag accacgctgc
     7681 tcgacggcgg cgcggtgctc accgcattcg atcccgacac cgccgcgcaa accggtgccg
     7741 catccgtgct cgttcccgat ggcggcctcg cggcgatcgc gctcgcgccg cgttttgccg
     7801 gcacgcgggt cgtcacgctc gaagatggat ccgtgatcgc gatcggtggc gacgccaacg
     7861 tcgcgcgcta cctgccgacg acgaacacgt gggcggtcgc accgcctgct ggcaccggcc
     7921 cgggcgcgat cgttgcgcca gtcctcatgc gactcggtga tggctcggtc ctcgtgctcg
     7981 gcggccgcac ggcaactgcc gaggcctgga tctatcggcc cgcgctcgtc gggccggcgt
     8041 cgggttccgt cgtcgcgctc cctgatggca gcacggaagg tgtgctcacg cccacggatc
     8101 cgacgctgct cga
//
LOCUS       DASUFX010000275        10185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_737884,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000275 DASUFX010000000
VERSION     DASUFX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737884"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFV99_10895"
     CDS             <1..415
                     /locus_tag="VFV99_10895"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5059859.1"
                     /translation="DPYQIAEARAAGADAVLLIVAALDEMQLAELLATAFDYQLDALV
                     EVHDLREAEVALKVGATLVGVNHRDLKTFTMDMTLTEKVARVVPPSTVLVGESGIKSP
                     ADARMLADAGAHAILVGEHLMRAPSPGAALRELLR"
     gene            412..1050
                     /locus_tag="VFV99_10900"
     CDS             412..1050
                     /locus_tag="VFV99_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5059860.1"
                     /translation="MTRIKICGVTLADDAARIASRGADFIGLNFWPKSKRYLAPERAL
                     MIASVARSTGNPKLVGVFVDPEIDEIQAIIATVDLDIIQLHGDETPDFVKKVSLAVYR
                     PVWKAIPIKSSRDLASLDVWPAEALLLDAPTPGRGGAGATFDWQLAREARERFPKINF
                     VLAGGLTPENVTTAIQTVQPWAVDVASGVEAGPGIKDPAKLDVFIGAARLAG"
     gene            1075..2295
                     /gene="trpB"
                     /locus_tag="VFV99_10905"
     CDS             1075..2295
                     /gene="trpB"
                     /locus_tag="VFV99_10905"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5059861.1"
                     /translation="MSTADEIAQLPEPVNGRFGEFGGQYVAETLMPAINELEHAWRTA
                     RRDSTFWSEVDDLLASYVGRPSRLYHAQRLSKDVGAQVYLKREDLNHTGAHKINNCIG
                     QAVLARRMGKRRLIAETGAGQHGVATATVAALFGLPCEVYMGAEDVVRQSLNVERMKL
                     LGAKVHAVESGTRTLKDAMNEALRDWVTNVGDTFYLIGSVAGPHPYPWMVRDLQSVIG
                     REARAQIQVQASRMPDALIACVGGGSNAAGLFAPFVGVPDVKLFGVEAAGEGVATGRH
                     AATLGAGRVGVLHGSKSYVLTHTDDARLGQIAHAHSISAGLDYPGVGPEHAYWKETGV
                     VTYVSRTDDEALAAIERLARSEGILCALETAHAVAALDEVKRALGKPDPIIIVGLSGR
                     GDKDMVTIAQRRQP"
     gene            2292..3101
                     /gene="trpA"
                     /locus_tag="VFV99_10910"
     CDS             2292..3101
                     /gene="trpA"
                     /locus_tag="VFV99_10910"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5059862.1"
                     /translation="MTAKTGARLRAAFDNAAAEKRAALVAYLTFGDPDPVTSIAVIEA
                     ACRAGADVIELGVPFSDPSADGPSIQRAMERALTAGGSLPSALSAVAELRARGITTPI
                     VLFGYYNPIFVMGPTVFAERAAKAGVDAVLTVDLPIDELAELASPLAANGVAVVPLVA
                     PTSTPERIARLGAFAAPFVYYISLTGVTGVRAAAPVDPARLDLIRNASHAPVAVGFGI
                     RTAADATRFAAIADGVVVGSALVDRVAAGSSSDAASRVAALVSELARAMPR"
     gene            3237..6041
                     /locus_tag="VFV99_10915"
     CDS             3237..6041
                     /locus_tag="VFV99_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5059863.1"
                     /translation="MKRLCFAIAVGLTGSLIGCGSGTPTDDDDQVQLPPSAVTQIEGL
                     LAEKAARTPAQQKISSQLLYAARGTFANALAPTKDPAKQITSLSQTDARGRVLVDIKG
                     DVSDGQIQALGGVLVGTSTVHHSTRAWLPLDRLEELAGNATVRAIRPALAATTDRAGI
                     PGGHPKFSLGTQAERVAAMQQASELWQGPATLATEPHAFASTGARTSEGVTAHGVERA
                     RKQYNVDGTGVTIGVLSDSDDHREQSILTGDLPASTITIPGQDGRPGAGEGTAMMEIV
                     HDMAPGANLVFATAFNGPDVMADNIRRLRFEYHCDVIVDDVIYFFESPYQDDIIAQAV
                     EDVVADGAVYVSSAGNEGNQNDGTSGTWEGDFKDAGTLATLPSGYTIHNFGDKTISDR
                     IEQQGGPLILHWSDPGTLDAPMSSNDYDLFVLDNDLRNVVVAATDLQDGAGMPFEYLG
                     YNLPPGFRVVVARHPNAETRAIRVALFRGELGLSTSGGTYGHSAAANALGAAAVDAAE
                     AAGGEFTAGPTTPVELFSSDGNRRVFYNRDGSPIKADKLTFASNGGEQRLKPDLAGAD
                     GVQTTLPPETGLEPFYGTSAAAPHIAAIAGLIKSAVPAATSTQIRNALKTGALDIEGA
                     GTDRDSGAGIAFAPDALKKAGAKAAVFLEQNSVTLTPTGSDAVLPGGSAQLRVQLINN
                     GLAKATAVNATLSSSSPYVTVTQPTSTYPNVFPGATATNPIAFAFTADAATPCGAQLP
                     LSLTINYTGIGPHPTVLPLTVQVGRPGATTVTSFVGSPVAIPDGVPSGVDIPLAVSAT
                     GPLASLRFSIDGSACTTDPGATTVGIDHTWVGDLHMTLVSPSGRAVTVIDGAGGAMNF
                     GHNFCQTLIDDAAPSSIQTVTPEMAPFTGWFKPANPLSAFIGEETAGTWTLHVMDSFP
                     ADGGNVRAFSLVTSGFSCTP"
     gene            complement(6045..7649)
                     /locus_tag="VFV99_10920"
     CDS             complement(6045..7649)
                     /locus_tag="VFV99_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX5059864.1"
                     /translation="MRALLVGVCLLCACGDDGVSTDPIMFGTMGPLAGDAGKGGFRFG
                     AATAATQIEDMNTNTDWWVWTAPTAMGGLGKGTFVGDAVKGYSKALDDVQLVKDMGLD
                     SYRFSIEWARIEPQRDVIDEAAITHYREQLMALKAAGIRPLVTIHHFSNPIWVADPRA
                     NACTGGPSDTNLCGFGNPVGGPLIVDELAEHAALLGQRFGDLVDEWGTLNEPVNYLIA
                     SYGIGYFPPGKMTIFTLLTDFVPVLRDYIAAHVKMYDALKANDTVDADGDGVAAVVGF
                     SLSVADWEPSRAHEPSTNPDDIAARDRLVYLFHYAIVDGLMSGMLDADLDGVGEEPHP
                     DWKNKIDWLGVQYYFRAGVTAQNPLIPAPVSLTPCTNGFDLGSCLKPHEQTHCVSQMG
                     YEFWADGLREILIAFKDRYPQLPLVVTEAGIATNVGARRAENVVRILEAIARARDENG
                     VDVRGYYHWSLTDNFEWAEGFAPHFGLYRVDYNSYARAATEGATVLGDIAKARTLTTD
                     QRKKYGGLGPMTHDEVETDPFCGQVK"
     gene            7720..8307
                     /locus_tag="VFV99_10925"
     CDS             7720..8307
                     /locus_tag="VFV99_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5059865.1"
                     /translation="MVKDPSPFTVLVRDLPVHRRFEVPATLVSDWLKGLPMRDALGAP
                     EGDPQAGEGVADLDLYADGTHAFAAGTFKGHLTVACSRCVGPVKLNLDETLRVTFMPP
                     GEIPEEDAEATSEEGPEVSEGDLDVFPFDGEKIDLEPLFREQFVLAVPFAPLCREDCK
                     GLCPQCGIDRNTGTCNCEKPIDPRLAALKGLKLPS"
     gene            8339..8539
                     /gene="rpmF"
                     /locus_tag="VFV99_10930"
     CDS             8339..8539
                     /gene="rpmF"
                     /locus_tag="VFV99_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5059866.1"
                     /translation="MALQKRRRGPARTAQQRSAWMKKSGENHMSVQACPNCGAPKIPH
                     RVCMACGQYRGETIVAKPAAEE"
     gene            8636..9655
                     /gene="plsX"
                     /locus_tag="VFV99_10935"
     CDS             8636..9655
                     /gene="plsX"
                     /locus_tag="VFV99_10935"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5059867.1"
                     /translation="MRVVVDAMGSDRAPAPEVAGAVRAARSNDILRITLVGDAPRLEA
                     LLANELRDRISIVHASEVVTMHDHPGQAFRQKPDSSMRRAVDLVAAAEGDAVVSAGNS
                     GAMLATALFVLGRLSGVERPAIVTVLPTPSGPLVLCDAGANVEPKPTHLAQFGVLGAA
                     YDRVVHGRARPRLGLLSNGSEPGKGNVLTREAHALLSEASGAFQYIGYVEGSDLFRGV
                     VDVIATDGFTGNVVLKTCEGIAEGLFGLVRQELEKTPLARLGSSLVAPALRGLAKRID
                     YTEIGGALLAGVTKPAVIAHGRSDANAIASAIRAAAQFAERALPAQLAASLADLKQAP
                     PTTGE"
     gene            9665..>10185
                     /locus_tag="VFV99_10940"
     CDS             9665..>10185
                     /locus_tag="VFV99_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059868.1"
                     /translation="MAKPKQIKVCLTNQGEDTETPWAIDLGPASGPKGSRKVRLINVP
                     FMHAKPTWGDVVVVSPVEDGFPTWDRNGVPWSKITSRLAEDGGRWAMIIDYAPHPDAK
                     AGDAFHELAHACAESDVVCEGAWGPRDGEPGRAYLAVKNELTDITLMRSLRAAELPCE
                     LIQIHPEPPKAKPA"
BASE COUNT         1738 a         3464 c         3297 g         1686 t
ORIGIN      
        1 cgacccgtac cagatcgcag aagcgcgcgc ggccggcgct gatgcggtgc tgctcatcgt
       61 tgcggccctc gacgagatgc agctcgcgga gctgctcgcc accgcgttcg actaccagct
      121 cgatgcgctc gtcgaggtgc acgacctccg cgaagccgaa gtggcattga aggtcggcgc
      181 aacactcgtc ggtgtgaacc atcgcgacct gaagacgttc acgatggaca tgacgctgac
      241 cgagaaggtc gccagagtcg tgccgccatc gaccgtgctc gtcggtgaga gcggcatcaa
      301 gagcccagcc gacgcgcgca tgctggccga cgctggcgcc catgcgatcc tcgtcggcga
      361 acacttgatg cgcgcgccct cgccaggtgc cgcgctgcgc gagctactcc gatgacgcgg
      421 atcaagatct gcggcgtcac gctcgcggac gatgcagcac gcatcgcgtc gcgcggtgcc
      481 gacttcatcg gtctcaactt ctggccgaag tcgaagcgct acctcgcgcc ggagcgcgca
      541 ttgatgatcg cgtcggtcgc gcggtcgacg ggcaacccga agctcgtcgg tgtgttcgtc
      601 gacccagaga tcgacgagat ccaggcgatc atcgcgaccg tcgacctcga catcatccag
      661 ctccacggcg acgagacgcc cgatttcgtg aagaaggtct cgctcgcggt gtaccggccg
      721 gtgtggaagg caattccgat caagtcgtcg cgcgacctcg cttcgctcga cgtgtggccg
      781 gccgaggcac tgctgctcga tgcgccgacg cccggccgcg gcggtgcggg cgcgacgttc
      841 gactggcagc tcgcccgcga ggcacgcgaa cgctttccga agatcaactt cgtgctcgcg
      901 ggcggtctga cccccgaaaa cgtcacgacg gcgattcaaa ccgtgcagcc gtgggcggtc
      961 gatgtcgcga gtggcgtcga agccggtcca ggcatcaagg atccggcgaa gctcgatgta
     1021 ttcatcggcg ccgcgcgact cgccggctaa cccacccacg tgataccgaa agctatgtcg
     1081 accgccgacg aaatcgccca actgcccgag ccggtcaacg gccgcttcgg tgagttcggc
     1141 ggccagtacg tcgccgagac gttgatgccc gcgatcaacg agctcgagca cgcgtggcgc
     1201 actgcccgcc gcgactcgac gttctggtcc gaggtcgacg atctgctcgc ctcgtatgtc
     1261 ggccggccat cgcgcctgta tcacgcgcag cgcctgtcga aggacgtcgg cgcgcaggtc
     1321 tacctcaagc gcgaggacct caaccacacc ggagcgcata agatcaataa ctgcatcggc
     1381 caggcggtgc tcgcgcgtcg catgggcaag cgccggctga tcgccgagac cggcgccggc
     1441 cagcacggcg tcgcgaccgc gacggtcgct gcgttgttcg gcttgccgtg cgaggtctat
     1501 atgggcgccg aggacgtcgt gcgccagtcg ctcaacgtcg agcgcatgaa gctgctcggc
     1561 gccaaggtcc acgcggtcga gagcggtacg cgcacgctca aagacgcgat gaacgaggcg
     1621 ctgcgtgact gggtcacgaa cgtcggcgac acgttctatc tgatcggttc cgttgccggc
     1681 ccgcatccct atccatggat ggtgcgcgac ctgcaaagcg tgatcggtcg tgaagcacgc
     1741 gcgcagatcc aggtgcaagc gagtcgcatg cccgacgctc tgatcgcctg cgtcggcggc
     1801 gggtcgaacg cggccgggct gttcgcgccg ttcgtcggcg taccggacgt gaagctgttc
     1861 ggtgtcgagg cggcaggcga gggcgtcgct accggccgcc acgcggcgac gctcggcgcc
     1921 ggtcgcgtcg gcgtactgca cggcagcaag agctacgtgc tcacgcacac cgacgatgcg
     1981 cggctcggcc agatcgcgca cgcgcactcg atcagcgccg gcctcgatta tccaggcgtc
     2041 ggccccgagc acgcgtactg gaaggagacc ggcgtcgtca cctacgtgtc gcgcacggac
     2101 gacgaggcgc ttgccgcgat cgagcggctc gcgcgcagcg aaggcatcct gtgcgcactc
     2161 gagaccgcgc acgctgtcgc cgcgctcgac gaagtgaagc gcgcgctcgg caagcccgat
     2221 ccgatcatca tcgttggcct gtcgggccgc ggcgacaagg acatggtcac cattgctcag
     2281 cggagacagc catgaccgcc aagactggag cccggttgcg cgcggcgttc gacaacgcgg
     2341 ccgccgagaa gcgcgccgcg ctggtggcgt acctgacgtt cggggatcca gatcccgtga
     2401 cgtcgatcgc agttatcgaa gcggcgtgtc gcgctggcgc agatgtgatc gagctcggcg
     2461 tgccgttctc cgatccatcc gcagacggtc cgtcgattca acgcgcgatg gagcgcgcgc
     2521 tcacagcagg tggcagtttg ccgtccgcgc tgagtgcggt agcggagctg cgtgcgcgcg
     2581 gcatcacgac gccgatcgtg ctgtttggct attacaaccc gatcttcgtg atgggcccca
     2641 cggtgtttgc cgagcgtgca gcgaaggcag gtgtcgacgc ggtcctgacg gtcgacctgc
     2701 cgatcgacga gctcgccgag cttgctagtc cgctggctgc gaacggcgtt gccgtcgtgc
     2761 cgctcgtcgc tccgacgtcg acaccagagc gcatcgcaag gctcggcgcg ttcgcggcgc
     2821 cgttcgtcta ctacatctcg ctcacgggcg tgaccggtgt tcgcgccgct gcgccggtcg
     2881 atccggctcg tctcgatttg atccgtaatg cgtcgcacgc acctgttgcg gttggtttcg
     2941 gcattcgcac ggctgccgat gcaacgcgct tcgcagcgat cgctgacggt gttgttgtcg
     3001 gatctgcgct ggtcgatcgt gttgcagctg gttcatcgtc cgatgccgcg tctcgcgttg
     3061 cagctctcgt gagcgagctt gctcgcgcga tgccgcgctg aacattcgcg ccgcgagatc
     3121 acgcgtgcgc gaacattcgc catcgggtag cgcagtgctt tgccagttgc gagcacgcaa
     3181 ccacgactca tcgttcgcgc tctatccggc gccgttcgtc ggcatgggag agtggaatga
     3241 agagactgtg tttcgcgatt gcggttggtc taacgggttc gttgatcgga tgcggctcag
     3301 gtacgccgac cgatgacgac gatcaggtcc agttgccgcc gagtgcggtg acccagatcg
     3361 agggactgct tgcagagaag gctgcgcgta caccggcgca gcagaagatt tcgtcacaac
     3421 tgctgtatgc ggcacgtggc acgtttgcga acgcgctcgc gccaacaaag gatcctgcga
     3481 agcagatcac cagtctttcg cagaccgatg cgcgcggtcg cgtgctcgtc gacatcaaag
     3541 gtgacgtgag cgatgggcaa atccaggcac tcggtggcgt gctggttggc acgtcgaccg
     3601 tgcatcacag cacgcgcgcg tggctgcccc tcgatcgact cgaagagctc gcgggtaacg
     3661 caacggtgcg tgcgatccga ccggcgctcg cggcaacgac cgatcgcgca ggcattcccg
     3721 gcggtcatcc gaagttctca ctcggcaccc aagccgagcg cgtcgccgcg atgcagcaag
     3781 cgagtgagct gtggcaagga ccggcgacgc tcgccaccga gccgcatgcg ttcgcgagca
     3841 ccggcgcacg tacgtcggaa ggcgtgacag cgcacggcgt cgagcgcgcg cgcaagcagt
     3901 acaacgtcga tggcacgggt gtgacgatcg gcgtgctctc cgacagcgac gatcaccgcg
     3961 agcagtcgat cctcaccggc gacctgccgg ctagcacgat cacgattcct ggacaggacg
     4021 gtcgtcccgg cgccggtgaa ggcaccgcga tgatggaaat cgtccacgac atggcgcccg
     4081 gcgcgaacct cgtgtttgcg actgcgttca acggtccgga cgtgatggcc gacaacattc
     4141 ggcggctgcg cttcgagtat cactgcgatg tcatcgtcga tgacgtgatc tacttcttcg
     4201 agtcgccgta tcaggacgac atcatcgcgc aagcggtcga agacgtcgtc gccgacggag
     4261 cagtctatgt ctcgtccgcc ggcaacgaag gcaaccagaa cgacggcacg tcgggcacgt
     4321 gggaaggtga cttcaaggac gcgggcacgc tcgcgacgct tccgtcgggc tacacgattc
     4381 acaacttcgg cgacaagacg atctccgatc gcatcgagca gcaaggtggc ccgctgatct
     4441 tgcactggtc ggatccgggc acgctcgacg cgccgatgtc gtcgaacgat tacgacctgt
     4501 ttgttctcga caacgatctg cgcaacgtgg tcgtcgccgc caccgatctg caggatggcg
     4561 ccggcatgcc gttcgagtac ctcggctaca acctcccgcc cggcttccgc gtcgttgttg
     4621 cgcgccatcc gaatgcggag acccgcgcga ttcgcgtcgc gctcttccgc ggcgagcttg
     4681 gcctgtcgac gagcggcggc acctacggtc acagcgccgc tgcgaatgcg ctcggcgccg
     4741 ctgcggtcga tgcggccgaa gcagctggcg gtgaattcac cgccggtccg acgacgccgg
     4801 tcgagctgtt cagctccgac ggtaaccgcc gcgtgttcta caaccgcgat ggcagcccga
     4861 tcaaagccga caagctcacg ttcgcctcga acggtggcga gcagcgcctg aagccggacc
     4921 tcgccggcgc cgacggcgtg cagaccacgc tgcctcccga gaccggcctc gagccgtttt
     4981 acggcacgtc ggcggccgcg cctcacatcg cagcgatcgc gggcctgatc aagtctgccg
     5041 tgccggccgc gacgtcgacg cagatccgca acgcgctcaa gacgggcgca ctcgacatcg
     5101 agggcgcggg caccgatcgt gacagcggcg cgggcatcgc gttcgcaccc gacgcgctca
     5161 agaaggcagg cgcgaaggcg gcggtgttcc tcgagcagaa cagcgtcacg ctcacgccga
     5221 ccggcagcga cgccgtcctg cccggcggct ccgcgcaact gcgcgtgcag ctgatcaaca
     5281 acggcctcgc gaaggcgacc gcggtgaacg cgacgctgag ctcgtcgtcg ccgtatgtca
     5341 cggtgacgca gccgacgtcg acgtatccga acgtgttccc gggcgccacc gcgacgaacc
     5401 cgatagcgtt cgcgttcacg gccgatgcag cgacgccatg tggcgcgcag ctgccgctct
     5461 cgctgacgat caattacacc ggcatcggtc cgcacccgac ggtgctgccg ctgacggtgc
     5521 aggttggccg cccgggcgcg acgacggtga cgtcgttcgt gggctcgccg gtcgcgattc
     5581 ccgacggtgt tccgagcggc gtcgacattc cgctcgcggt cagcgcgacc ggaccgctcg
     5641 ccagcctgcg cttctcgatc gacggctcgg cgtgcacgac ggatccgggt gcgacgacgg
     5701 tcggcatcga tcacacgtgg gttggtgatc tgcacatgac gctggtctcg ccgagcggaa
     5761 gagccgtgac cgtgatcgac ggggcaggtg gcgcgatgaa cttcggccac aacttctgcc
     5821 agacgctgat cgacgacgca gccccgtcct cgatccagac cgtgacgccg gagatggcgc
     5881 cgttcaccgg ttggttcaag ccagcaaacc cgctgtccgc gttcatcggc gaggaaacgg
     5941 ccggcacgtg gacgctccac gtgatggaca gcttcccggc cgatggcggc aacgtccgcg
     6001 cgttctcgct cgtgacgagc ggcttcagct gtacgccgta agcgtcactt gacctgaccg
     6061 cagaacggat cggtctcgac ctcatcgtgc gtcatggggc cgagaccgcc gtacttcttg
     6121 cgctggtcgg tcgtcagcgt acgtgccttc gcgatgtcac cgagcacggt cgcgccctcg
     6181 gtggccgcac gagcgtacga gttgtagtcg acgcgataga ggccgaagtg cggcgcgaag
     6241 ccttcggccc attcgaagtt gtcggtcaag ctccagtggt agtagccacg gacgtcgacg
     6301 ccgttctcgt cccgcgcacg cgcgatcgct tcgaggatgc gcacgacatt ctcggcgcgt
     6361 cgcgcgccga cgttcgtggc gatgcccgct tcggtcacga cgagcggcag ctgcggatag
     6421 cggtccttga acgcgatcaa gatctcgcgc agcccatcgg cccagaactc gtaacccatc
     6481 tgcgagacgc agtgcgtttg ctcgtgtggc ttcaggcacg agccgagatc gaagccgttc
     6541 gtgcacggcg ttagcgacac cggcgccgga atgagcgggt tctgcgcagt aacgccggcg
     6601 cggaagtagt actgcacgcc gagccagtcg atcttgttct tccagtcggg atgcggctcc
     6661 tcgccgacgc cgtcgaggtc ggcatccagc atgccgctca tcaacccatc gacgatcgcg
     6721 tagtgaaaca gatagacgag gcggtcgcgc gccgcgatgt catcgggatt cgtcgacggc
     6781 tcgtgcgcgc gcgacggctc ccagtccgcg accgacagcg agaatccgac gaccgcggcg
     6841 acgccatcgc cgtccgcatc gaccgtgtcg ttcgccttca gcgcgtcgta catcttcacg
     6901 tgcgccgcga tgtaatcgcg cagcaccggc acgaagtcgg tgagcagcgt gaagatcgtc
     6961 atcttgcccg gcgggaagta accgatgccg tagctcgcga tcagatagtt caccggctcg
     7021 ttgagcgtgc cccattcgtc gacgaggtcg ccgaatcgct ggccgagtaa cgccgcgtgc
     7081 tccgcgagct cgtcgacgat caacggcccg ccgaccggat tgccgaagcc gcacaggttc
     7141 gtatcgctcg ggccgccggt gcatgcattc gcgcgcggat cggcaaccca gatcggattc
     7201 gagaagtgat ggatcgtgac gagcggccga atgccggccg ccttcagcgc catcagttgt
     7261 tcgcggtagt gcgtgatcgc ggcttcgtcg atcacgtcgc gttgcggctc gatgcgcgcc
     7321 cactcgatcg agaatcgata gctgtcgaga cccatgtcct tgacgagctg cacgtcgtcg
     7381 agtgccttcg aatatccctt caccgcgtcg ccgacgaacg tgcctttgcc caacccgccc
     7441 atcgcggtcg gcgcggtcca cacccaccaa tcggtgttgg tgttcatgtc ctcgatctgt
     7501 gtcgccgcag tcgccgcgcc gaagcgaaac ccgcctttgc cggcatcacc ggcgagcggt
     7561 cccatcgtgc cgaacatgat gggatcggtg gacacaccgt cgtcgccgca cgcgcaaagg
     7621 agacacactc cgacgagaag cgctcgcatt ggcgagcatc ctaaccaaaa cgcgcagctt
     7681 gacactcgag atcgcgttcc tatactcgcg cccttcgtca tggtgaagga tccttctccg
     7741 ttcacggtgc tggtacgcga cctgccggtc caccgtcgct tcgaggtgcc tgccacgctg
     7801 gtcagcgatt ggctcaaggg cctgccgatg cgcgatgcac tcggtgcacc tgagggcgat
     7861 ccacaagcgg gcgagggcgt cgccgacctc gatctttatg cagacggtac gcacgccttc
     7921 gcggcgggca ccttcaaggg ccacctcacc gtcgcctgca gccgctgcgt cgggccggtg
     7981 aaactgaacc tcgatgaaac gctccgcgta accttcatgc cgccgggcga aatccccgaa
     8041 gaggacgccg aggccacctc cgaggaaggc cccgaggtca gcgagggcga cctcgacgtc
     8101 ttcccctttg acggcgagaa gatcgaccta gagccgctgt tccgcgagca attcgtcctc
     8161 gccgtcccgt ttgcccccct gtgccgggaa gactgcaaag gcctgtgccc gcagtgcggg
     8221 atcgaccgaa atacaggtac ctgcaactgc gagaaaccca tcgatccgcg cctcgccgca
     8281 ctcaagggct tgaaattgcc ctcgtgacct ggcacaaagc ccgtcccgga gtcttcccat
     8341 ggcactgcaa aaacgacgtc gcggcccagc ccgcaccgcc cagcaacgct cggcgtggat
     8401 gaagaagtca ggcgagaacc acatgagcgt tcaggcctgc ccgaactgtg gtgcgcccaa
     8461 aattccgcac cgcgtctgca tggcgtgcgg tcagtatcgc ggcgagacga ttgtcgcgaa
     8521 gcccgctgcc gaagagtagt cgtcgtccgc tccatgccat tcgcggcctt tcgctttcgg
     8581 gatcgctagc cggtcgcgca aagcaatcgc cgccgccatc ggctagtctc gccgcgtgcg
     8641 agtcgtggtc gatgcaatgg gcagcgatcg cgcgcccgcg cccgaagtgg ccggcgcggt
     8701 tcgcgcagcg cgctcaaacg acatccttcg catcacgcta gtcggcgatg cgccgcgtct
     8761 cgaggcgctg ctcgcgaacg agctccgcga tcgcatcagc atcgtgcacg cgtccgaggt
     8821 cgtcacgatg cacgaccatc cgggccaagc gtttcgccag aagcccgact cgtcgatgcg
     8881 ccgcgcagtc gatcttgtcg ccgccgccga gggcgacgcg gtcgtctccg ccggcaacag
     8941 cggcgcgatg ctcgcgaccg cgctgttcgt gctcggccgt ctgtccggcg tcgaacgccc
     9001 agcaatcgtc accgtgctac cgacaccatc gggtccgctc gtcctttgcg atgccggtgc
     9061 gaacgtcgag cccaagccaa cgcacctcgc acaattcggt gtgctcggcg ccgcgtacga
     9121 tcgcgtcgtg cacggccgcg cgcgtccacg cctcggcctt ctctccaacg gctccgagcc
     9181 cggcaaaggc aacgtgctca cgcgcgaagc gcacgcgctg ctctccgaag catcgggcgc
     9241 gttccagtac atcggctacg tcgaaggcag cgacctcttc cgcggcgtcg tcgacgtgat
     9301 cgcgacggac ggcttcaccg gcaacgtcgt cctcaaaacc tgcgaaggca tcgccgaagg
     9361 tctcttcggc ctcgtgcgcc aggagctcga gaagacgccg ctcgcgcgcc tcggctcctc
     9421 gctcgtcgcg cccgcactgc gcggtctcgc gaaacgcatc gactacaccg aaatcggcgg
     9481 cgcgctgctc gctggtgtca cgaagcccgc cgtgatcgcg cacggccgca gcgacgcaaa
     9541 cgcgatcgca tccgcgatcc gcgccgcagc acaattcgcc gagcgcgcgc tgcccgcaca
     9601 actcgccgct tcgcttgccg atctaaaaca ggcgccgccg accaccggcg aataagatgg
     9661 tcacgtggcg aagccgaagc agatcaaggt ttgcctgacg aaccaaggcg aagacaccga
     9721 gacgccatgg gccatcgatc tcggtccggc ctccggaccc aaaggctcgc gcaaggtccg
     9781 tctcatcaac gtgccgttca tgcacgccaa acccacgtgg ggcgacgtcg ttgtcgtctc
     9841 gccggtcgaa gacggctttc cgacctggga ccgcaacggc gtgccgtggt ccaagatcac
     9901 gtcgcgcctc gccgaggacg gtggtcgctg ggcgatgatc atcgactacg cgccgcatcc
     9961 cgacgccaag gccggcgacg cctttcacga gctcgcgcat gcgtgcgcgg agtccgacgt
    10021 cgtatgcgaa ggcgcgtggg gcccgcgtga cggcgagccc ggccgcgcgt acctcgcggt
    10081 gaagaacgag ctcaccgaca tcacgctgat gaggtcactg cgcgcggccg agctaccgtg
    10141 cgagctcatc cagattcatc ccgagccacc gaaggcgaag cccgc
//
LOCUS       DASUFX010000276        17008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_43120,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000276 DASUFX010000000
VERSION     DASUFX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43120"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFV99_10945"
     CDS             <1..657
                     /locus_tag="VFV99_10945"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX5059869.1"
                     /translation="RVRLDMEIEGNRDWIADSIQVGVGAGATIEARSNIEIFVGPSWI
                     ERTDPMQYVAQATDDAGRAHYVFARIHQTTVALTTRVNWTFSPRLSLQAYAQPFIAAG
                     RYSELKDVETPDAHRFADRFHVLSGSEYAIDSGTVRVTHDGTYSFERPDFNFDELRST
                     VVLRWEYRPGSTIFAIWSHGRANAVGDGRFDLINDVANIGDAPGEHTVMVKANYWMGL
                     "
     gene            714..1349
                     /locus_tag="VFV99_10950"
     CDS             714..1349
                     /locus_tag="VFV99_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5059870.1"
                     /translation="MSITRRILESAQSGLSKLTSLVIVDDEPLSHVESAALQSELTAR
                     KAAREKSQKQSLDNPLAKQALANPGARAARDKAAKDRAARVHRERDEREAKQRAAADE
                     AFRRMKEQAAKGGTTSSYSSTSSSSTGTGSARPPRPGSVDAQLAEWYRQLDLQPGAEM
                     SQIKTSYRQLMRKYHPDMHAGNPQRQKAATELSMRVTAAYNGLVSHLESKK"
     gene            1424..2275
                     /locus_tag="VFV99_10955"
     CDS             1424..2275
                     /locus_tag="VFV99_10955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5059871.1"
                     /translation="MDIESSASKTALMVCAYRARASRWQKPLFVDPWAEAIAGRDGHE
                     IADNLDKRFPPMELWLALRVAYLDRLVGQAVDRLSIRQVVILGAGYDTRAARLPRAGV
                     TFFEVDHPATQASKRERLASLDKYPVDAARYVTCNFEREDPIERLVANGFTTREPALV
                     IWEGVVPYLTEGAVRATATRLATGLDPRSLVAFDFVGKKFASGASVSDADHATRAMVG
                     ELGEPIRYGSDDILPLLYDCGFRWVRQLDFNELALEMLGDYQRDRQFRFQHLALAAAR
                     TPVAEWP"
     gene            2266..4410
                     /locus_tag="VFV99_10960"
     CDS             2266..4410
                     /locus_tag="VFV99_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059872.1"
                     /translation="MALSSQDDPARRSESGALRFGRARRLATWAKPYAPTAVIVALAL
                     FIVWAFAATTLHYAGSMGLPLDDSYIYLTYAKQFGRAEPFTYFPGGGYSAGSTSVLWP
                     MLLAPFWTLGARGHALVWVSYGMCAVLYAAVAISVYRFVAKIRDPLSGLLGAVMVLVI
                     APFAWCALSGMEVALAAALLVATLLLLLREPNQGRPSGLFIACLAAASLSRPEATLLV
                     GAIVAVRISQRLRARNWRAAAWWLAPLAPVTLWLLANKLIAGNFFPNTGVAKSHFYLP
                     GFDWAYWRDAVTTLSGRMLRSLFWDKTSPLIWPRLVAALFLVGAVRVGIWAWREKQFL
                     VGTLVIVAPFVMMLAVIASSGLWSFQNYRYIAPAFPLLLIPVAIALSPAGKSVALRRA
                     WHAGGIALVILFARSARPGLVADMRLFAQGGMDTNTQVVAIGKYLHRKLPDARVMFHD
                     AGAIAYYGDGRVYDMLGLVTNHQAGIANNGPGARFEFLESLPTERRPTHFAYYPGWMG
                     TPEFFGEALMHTYLRPGIEGRRLVGEGDMQVLTANWDHAHSGERPLNDHTGWTITDRV
                     DIADIASERSHGWVGRMGRRKFGDPTARWSIVERETINGLVIDGGRTIRAGGERFTVH
                     VDPGKPTRVVLRTGGQKSYGWHEAIDKPVTITLFVGKKQLGQLVIAPPAGQFSELTFN
                     LPAHALPTANTELHVEANGIYRVFHWFVLQPE"
     gene            complement(4446..5483)
                     /locus_tag="VFV99_10965"
     CDS             complement(4446..5483)
                     /locus_tag="VFV99_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5059873.1"
                     /translation="MLLPGDLDPFFALSPDVCCVMGTDGTIHAANAAWSTVLGYASND
                     VVGAAFFLLVHSDDRARVQADLTHEGTARFGARVRSKDGQYRLLHWTSTTKSDARKIF
                     AIGHAEPTTLGSSERLLSLGQIALGVVHDLKNVLVHPLGLHLQRMERGLEAQAPEKVR
                     AAIGSMRDVLDDGQAAIDRMLQFTQPRNASTRIRADVENLAWRATEIARAYARSIAPK
                     QEIELAYDQGRPKRVDCDSFELLAALVNVLFNAIDAVADAGGKVIVRTGTSPKRVWLE
                     VVDNGPGMSTEVRDRIFEPFFTTKRDGIGIGLAMVKKCIESHAGTIEITSAPGAGTTF
                     RIELPASPAVI"
     gene            complement(5538..6320)
                     /locus_tag="VFV99_10970"
     CDS             complement(5538..6320)
                     /locus_tag="VFV99_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5059874.1"
                     /translation="MTEGATVTHETRFVRSSDCRSHFCSTNKSRKRCVEIHSRRNLCA
                     FGPHLGPNYRRMDCCDARPMPPPRKPAIALLSDHELFRQVVAQRLADQGFSPVLQLSS
                     KDKLVAAAKRRRVDIAIVDIDHDSEDTLSLVRSLHAELPALHVIVLATPVQQSADDGV
                     VLEVGGIDRANAVTGIAVGLRNGDEKAPHRHWSRITERQREVMRWLAVGLDNAAIGGK
                     LRIGERAVKAHVSSLLGLFGLANRTQLALMADRAGLRPPNRR"
     gene            complement(6335..7420)
                     /locus_tag="VFV99_10975"
     CDS             complement(6335..7420)
                     /locus_tag="VFV99_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5059875.1"
                     /translation="MHLLPTTMVGSYPRPLWFTQQLAGRDVLEAFKVASHAEAFHDAT
                     RVVIKDQEEAGLDILTDGQMWFDDYHMGIGSFLWYWLERTKGFSAEKLPHPARAKAKG
                     RDIWALDEAGGVAVEGAIERGPVRMALFYNWAQRHTQRPIKACVGAGPVQLSTLAYFR
                     SGPVKNRYDLSRALADVFRAEIKELVEAGCKHIQLEDLGAWMPHLSGDKDWDWVSDTV
                     DRTMADIPQGVERAWHFCMGNAWGNKLEGMTAGGYREVLPHYHGVKVDAYVLDFAARE
                     MEDADLLRKLPSDKKVHVGVIDVRMLEIEHPEQVAERIRKVLKHIEPERVTLTTDCGL
                     KQLPRVVAIQKLRALTEGATIVRRELQ"
     gene            7527..7958
                     /locus_tag="VFV99_10980"
     CDS             7527..7958
                     /locus_tag="VFV99_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059876.1"
                     /translation="MRSALACLAALVPAVTHADPAPDRDGSVWTDPELGFGAGLAGLG
                     YAGSIAWARQRTGDQGALWIPVLGPWLELANSSDCGNGEMSCPHGNATRSVLIVSGLV
                     QLAGVGLMAHALTKPHHKKPPVVIAPTMTGMGGGVQIRGRF"
     gene            complement(7955..9580)
                     /locus_tag="VFV99_10985"
     CDS             complement(7955..9580)
                     /locus_tag="VFV99_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX5059877.1"
                     /translation="MFRAGLVALALLTAQPAGARADDTSSSDKLRILYSTRFTFTDTG
                     VPLVTVEIMGGKKDIKLRAKGGVVVRPDGSGGSAIEADGGDSWTVTVEGAKPAVIQEW
                     TVVETLGPDDATGISTALTRWKDRGFDPRSFEIGTVFGTGGELIDTREVRIAIDPVAV
                     GKGTARAAEHAKKYGVKTLVHDELVRRPAGTIVAKSGSTTIRNASVLWFQPKKQTETL
                     TVADVPTGTGGSQLQTGIEDRRYWGNVYVTLDHDGSLLAANAVSEDKLLAGLVPAEMY
                     PDAPAAALEAQAIAARTELLQKIGRRNLTDPFLLCSTQQCQVYAGAGKEDPRTTKAVE
                     KTRGMVLLRDGGGLVDVRYSATCGGHSEDNEAIWGGDPDPSLRGRRDDPKGGMSKVTS
                     IQAFLAEDPKNFWCGRVEKARNKFRWTEKISVDDMTLRISTDYPELGRVKALTAKQRG
                     VSGRIQILTITGEKSTVDVSGDLRIRRLLGGLKSTLFDVKRDGNTFVFEGAGFGHGVG
                     MCQLGAIGMADAGKTSEQILGHYYRGTHLHRLY"
     gene            complement(9627..10442)
                     /locus_tag="VFV99_10990"
     CDS             complement(9627..10442)
                     /locus_tag="VFV99_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5059878.1"
                     /translation="MIVARSVAEFLAHPLGHCVVGSTFVIWCAAPDLAGSIQWGTLDE
                     RCVREMLSVAEFVEHPDLASNGRVLMDCRAVERVDVDALLAFTTTARTQLPSWSARIE
                     RQVVILPGGLSGILLAGALPSLAPTHFLRFVDTLDAALHVLDHPGARAAHVAAEAAAT
                     EARRDGVLLTRVRSALATMLVGATIEQCASALNMSTRTLQRELKRLGTSFSDELRRVR
                     LAAAEDLLFGTELKIDAIADRVGLGSASRMSALLRREHNLTPAALRARGRTRT"
     gene            complement(10450..10905)
                     /locus_tag="VFV99_10995"
     CDS             complement(10450..10905)
                     /locus_tag="VFV99_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059879.1"
                     /translation="MIGIPVGLVFANAMEWVFHKHVLHGLGKRKNSFWSFHWHEHHRA
                     SRRHAFLDDAYRGSLLRWNAQSKEVLALAAGALVLLPLVPIAPFFVGTVWFCEFDYHR
                     KHRRAHLEPEWAREHLPWHYAHHMGHNQDANWCVKRPWMDTVMGTRSAM"
     gene            11063..11908
                     /locus_tag="VFV99_11000"
     CDS             11063..11908
                     /locus_tag="VFV99_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5059880.1"
                     /translation="MEISPVTRARVELHVTDRIEEFVSDPVGRCVLGPTYLIWCATPD
                     LQGAIIWSTIDELALRNMMAIGRFILHPALAARRRLVVDCRDVTRVGADVLLGFAELA
                     HDRVSVWATSVERQAVIVPGGLAGLLIGGALPSVGALQPLRFSHDLESALAFVDHPAA
                     RVAHELANEIAASQRGKAILLTRLHAQLAHDLTDASVESSASALGLGTRTMQRALARL
                     GTSFSDELRRCRVIAAEALLLHSDLKIEAIALRVGFGNASRMSATLRRVLNVTASGLR
                     ARARH"
     gene            12001..12477
                     /locus_tag="VFV99_11005"
     CDS             12001..12477
                     /locus_tag="VFV99_11005"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5059881.1"
                     /translation="MIMLQRLLRRLPNGWALVPLRLVVGFGFAYHGYAKLARGPEHFA
                     TILSAMGVPGPHTMAWLTTFVELFGGAALMLGAFVVPVSVPLIIVMATAIFGVHAQYG
                     FSSVRLKAISDAGAQFGPIGYELDLVYIAALVALAIAGPDRLSLDRWLAVRRSRRA"
     gene            12568..13047
                     /locus_tag="VFV99_11010"
     CDS             12568..13047
                     /locus_tag="VFV99_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059882.1"
                     /translation="MHDVQRDYAPLILFYAGDRRHLFSLFRLADDSVAAINRYVNAGV
                     VLVALDVDEVVGHLQLIQTDPWQINSLAVVPSHRRRAIGRALVETAVKHARGVGVRTI
                     EVATAAADLGALRFYQRLGFRMTRIERDAFTESAGYPADAMVDGVPLLDRVWLERAI"
     gene            13126..13626
                     /locus_tag="VFV99_11015"
     CDS             13126..13626
                     /locus_tag="VFV99_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HEX5059883.1"
                     /translation="MLSNSSDLGARFSTREAVDARIRQTHVLGTAVLRYGVVFLLVTI
                     GMAKFFAFEAEGIKPLVANSPLLSWMLGVFGVRGTADVIGSIEIVAGVAMATRRFAPV
                     LSALGSAIGVLIFLTTLSFLFSTPGALSLKHPASAFLMKDIVLLGACIATGSEALREA
                     HVRRRV"
     gene            13695..14531
                     /locus_tag="VFV99_11020"
     CDS             13695..14531
                     /locus_tag="VFV99_11020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5059884.1"
                     /translation="MFVDIATALRIEHAEASMMRDMVNALVGTSRAPAAFVRDLGMGT
                     ATYVRASSPLNKVIGAGIDAPIDAALLGATERAFDSKGEPVRVELSTLADPQTYPLLA
                     ERGYRLLGFENVLARSLVGVGPTPVANVRIETVTASVLSEFRDTTIEAAAHPDDTGVV
                     LDTFSRDVIATAVDDSLGSHGFTRYLAYRDGAIAGAASMTMHGDVAVLAGSGTLVAHR
                     RRGVQAALLATRLADACAAGATLAVITTAAGTQSQANVMKLGFALAYARAVLVRAPRP
                     AA"
     gene            complement(14534..>17008)
                     /locus_tag="VFV99_11025"
     CDS             complement(14534..>17008)
                     /locus_tag="VFV99_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5059885.1"
                     /translation="RDRELGVLMGLFEEAVAEPMARAVLVTGVAGIGKSRVRYELLRR
                     IKAREEPVEIWIGRGDPLHTGSPFGMIAPALRRAAGILDGEPVEVRRQKLRARVMRNA
                     SAEPTRTVMFLGELVGVPFPDDDSVELRAARQDALLMSDQVRRAFEHFLAIETAAQPV
                     VIVLEDLHWGDLPTTKLIESALRALPDRPWFVAAFARPEIQEMFPLLWKDRGVHDLRL
                     DELTKRGSEKLVRQVLGDRANDALVARLVEQAGGNAFYLEELIRAVAEGKGDAMPETV
                     LAVVQGRLERLEVDARRVLRAASVYGAQFWRGGVAKLLGGEARDSSVRAWLDELVERE
                     LIAMAPGSRFTDEDEYLFRHAIVREAAYAMLTDSDRKLGHRLAGEWLQYAGETEAMVL
                     AEHYERGGAPVRAALWFGPAVDQALAAGDFRAAAQRAERGVACGASGETLGLLRRAQG
                     EAHKWCGEFVEAERYSLQAMELLLRGSAPWFAAAGEAAEACGKLGDLDQLATIARALT
                     EAPDLEGELAARVTATATCAFQLYNHGKYQLASEMVDQIDRVAAEVHDPRILARIYQT
                     RSSRAMFSGDSGAYVVSEQAAALAFEQAGDLRYACMQHGHVGYAYLEIGAYAEAERWL
                     RKALDGGLRMGLQNVVATAKHNLGRALQHQGKLDEAAAIETEAVEAFAAQGDRRLESA
                     SRTYLASILAMRGELSVAEELVRQALKTVQAASRPALLADLASMLLKQGRHHEALIAA
                     REAHDLLEMLGGVEEGESQIRLMVAEALRASGDAEGAREAIRRARERVLARATKITDA
                     EWRTSFLTNLPENARTLALATELLDA"
BASE COUNT         2754 a         5647 c         5593 g         3014 t
ORIGIN      
        1 cgcgtgcgcc tcgatatgga aatcgaagga aatcgcgatt ggattgcgga ctcgatccag
       61 gtcggcgtcg gcgccggtgc gacgatcgag gcgcgctcga acatcgagat cttcgtcggg
      121 ccgagttgga tcgagcgcac cgacccgatg cagtacgtcg cgcaggcaac cgacgatgcc
      181 ggccgcgcgc actacgtgtt cgcgcgcatt caccagacca cggtcgcgct gacgacgcgc
      241 gtgaactgga cgttctcgcc gcggctctcg ctccaggcat acgcgcagcc gtttatcgcg
      301 gctggcagat acagcgagct caaggatgtc gaaacaccag acgcgcatcg gttcgcggat
      361 cgcttccacg tgctgtccgg tagcgagtac gcgatcgaca gcggcacggt tcgcgttacg
      421 cacgatggca cctatagttt cgagcgcccc gacttcaact tcgacgaatt acgctccacg
      481 gtcgtgctgc ggtgggaata ccggcctggc tccacaatct ttgcaatctg gagccatggg
      541 cgcgcgaatg ctgtcggcga cgggcgattc gaccttatca acgacgttgc caacatcggt
      601 gatgcgccgg gcgagcacac ggttatggtc aaagcgaatt attggatggg cctctagggc
      661 aagggctagt agtaagcaaa ggcggtgtgc ccgattgatg ctagcttagc ggcgtgagca
      721 tcacgcgccg catcctcgag agcgcgcaat ccgggttgtc gaaactgacg tcgctcgtca
      781 tcgtcgatga cgagccgctg agccatgtcg agagtgcggc gcttcagtcg gagctgaccg
      841 cgcgcaaggc cgcgcgcgag aaatcgcaaa agcagtcgct cgataatccg ctcgccaagc
      901 aggcgctcgc caatcccggc gcccgcgccg cccgcgacaa ggcggcgaaa gatcgcgcgg
      961 ctcgcgtcca tcgcgaacgt gacgaacgcg aagccaagca gcgcgccgct gccgacgagg
     1021 cgtttcgccg catgaaggag caggctgcga aaggcggcac caccagcagc tactcgtcga
     1081 cgtcgtcatc gagcacgggt accggcagcg cccgcccgcc caggccgggc agcgtggatg
     1141 cgcagctcgc cgaatggtac cgccagctcg atctccagcc gggcgccgag atgtctcaga
     1201 tcaagacctc gtaccgccag ctgatgcgca agtaccaccc ggatatgcac gcgggaaatc
     1261 cgcagcgtca gaaggccgcg accgagctgt cgatgcgggt cacggccgca tacaacggcc
     1321 tggtctcgca tctggaatca aaaaagtaac agtcgacggc ccgggggcgg catgacggag
     1381 tcattgcact gctctgacga agtgatcgtg ttatagcccg ggcgtggata tcgagagctc
     1441 tgcgtcgaag acagcgctga tggtgtgcgc gtatcgcgcg cgggcgagcc gctggcaaaa
     1501 gccgctgttc gtcgatccgt gggctgaggc gatcgccggc cgcgacggtc acgagatcgc
     1561 cgacaacctc gacaagcggt tcccaccgat ggagctctgg ctcgcgctac gcgtcgccta
     1621 tctcgatcgc ctcgtcggtc aagcggtcga ccggttgtcg attcgccagg tcgtcatcct
     1681 cggcgccggt tacgacacgc gcgctgcacg gctgccacgc gcgggcgtca cgttcttcga
     1741 ggtcgatcat cccgcgacgc aagcgtcgaa gcgcgagcga ctcgcgtcgc tcgacaagta
     1801 tccagtcgat gcggcccgct acgtgacatg caacttcgag cgcgaagatc cgatcgagcg
     1861 cctcgttgcc aacgggttca ccacgcgcga gcctgcgctc gtgatctggg aaggcgtcgt
     1921 gccgtacctg accgagggcg cggtgcgggc cacggcgacg cggctcgcga ccggactcga
     1981 tccgcgctcg ctggtcgcgt tcgatttcgt cggcaagaag ttcgcctccg gtgcgagcgt
     2041 ttcggatgcg gaccacgcga cgcgcgcgat ggtcggcgag ctcggcgaac cgattcgcta
     2101 cggctccgac gacatcttgc cgctgctcta cgactgcggc ttccgctggg tgcgtcagct
     2161 cgacttcaac gagctcgcgc tcgagatgct cggtgactat cagcgtgacc gccagttccg
     2221 ctttcagcac ctcgccctgg ctgccgcgcg cacgccggtg gccgaatggc cctgagcagt
     2281 caggacgacc ccgcgcgccg gagtgaatcc ggcgcgctcc gctttgggcg cgcgcgccgt
     2341 ctcgcgacat gggcgaagcc ctacgcgccg acggccgtca tcgtcgcgct cgcactgttc
     2401 atcgtgtggg cgttcgcggc gacgacgctg cactacgccg gctcgatggg gctgccgctc
     2461 gacgacagct acatctacct gacctacgcg aagcagttcg gccgcgccga gccgttcacg
     2521 tacttcccgg gtggcggcta ctccgcaggc tcgacgagcg tactgtggcc gatgctgctc
     2581 gcaccgttct ggacgctcgg cgcgcgcgga catgcgctcg tctgggtctc gtacggcatg
     2641 tgcgcggtgc tctatgcggc cgtcgcgatc agcgtctatc ggttcgtcgc gaagattcgc
     2701 gatccgctgt cgggcttgct cggcgccgtg atggtgctcg tgatcgcgcc gttcgcgtgg
     2761 tgtgcgctgt cgggcatgga ggtcgcgttg gccgcggcgc tgctcgtcgc gacgctgctg
     2821 ttgctcctgc gcgagccgaa tcagggccgc ccgtcggggc tgttcatcgc ctgtctcgcc
     2881 gccgcatcgc tgtcgcgtcc cgaagcgacg ctgctcgtcg gtgccatcgt ggccgtgcgc
     2941 atcagtcagc ggctgcgcgc gcgcaactgg cgtgcggccg cctggtggct cgcgccgctc
     3001 gcgccggtca cgctgtggct gctcgcgaac aagctgatcg ccggcaactt cttcccgaac
     3061 accggcgtcg ccaagagtca cttctatctg cccggtttcg actgggcgta ctggcgcgac
     3121 gccgttacta cgctctcggg tcgcatgctg cgcagcctgt tctgggacaa gacgagccca
     3181 ctgatctggc cgcggctcgt ggccgcgctg ttcctcgtcg gtgcggtgcg cgtcggcatc
     3241 tgggcatggc gcgagaagca gttcctcgtc ggcacgctcg tgatcgtcgc gccgttcgtg
     3301 atgatgcttg ccgtcatcgc gtcgtcggga ttgtggagct ttcagaacta tcgctacatc
     3361 gcgccggcgt tcccgctgct gctcattccc gtcgcgatcg cgctgtcacc ggcgggcaag
     3421 tcggtcgcac tgcgtcgcgc gtggcacgcc ggtggtatcg cgctcgtgat tctgttcgcg
     3481 cgttcggcgc ggccgggcct cgtcgccgac atgcgcctgt ttgcacaggg cgggatggac
     3541 acgaacacgc aggtcgtcgc gatcggcaag tacctccatc gcaagctgcc cgacgcgcgc
     3601 gtgatgtttc acgatgccgg cgcgatcgcg tactacggcg acggacgcgt ttacgacatg
     3661 ctcggcctcg tcacgaatca ccaggctggc atcgcgaaca acggtccggg tgcgcgcttc
     3721 gaattcctcg agagcttgcc gacagaacgc cggccgacgc acttcgcgta ctacccgggt
     3781 tggatgggca cgccagagtt cttcggcgag gcgctgatgc acacctatct gcggcccggc
     3841 atcgaaggcc gccggctcgt cggggaaggc gacatgcagg tcctcaccgc gaactgggat
     3901 cacgcccata gcggagagcg gccgctcaac gatcacacgg gctggacgat cactgatcgc
     3961 gtcgatatcg ccgacatcgc gagcgagcgc tcgcacggct gggtcggccg catgggcagg
     4021 cgcaagttcg gcgatccgac ggcgcggtgg tcgatcgtcg agcgcgagac catcaacggc
     4081 ctcgtgatcg acggcgggcg cacgatccgt gcgggtggtg agcgcttcac cgtgcatgtc
     4141 gatccaggga agccgacgcg ggtcgtgctg cgcaccggcg gtcagaagag ctacggctgg
     4201 cacgaggcga tcgacaagcc cgtgacgatc acgctgttcg tcggcaagaa gcagctcggc
     4261 cagctcgtga tcgcgccgcc tgccggccag ttctccgagc tgaccttcaa tttgcctgca
     4321 cacgcgctgc ccactgcaaa caccgagttg cacgtcgagg cgaacggcat ctaccgcgtg
     4381 tttcactggt tcgtgctgca gcccgagtga tacgcgcgcg cggcacccga tcgggtgacc
     4441 agcgcttaga tcacggcggg tgatgcgggc agctcgatcc ggaacgtcgt accggcgccg
     4501 ggcgcgctcg tgatctcgat cgtgccggcg tggctctcga tgcacttctt caccatcgcg
     4561 agtccgatcc cgatgccatc gcgcttcgtc gtgaagaacg gctcgaagat gcgatcgcgc
     4621 acctccgtgc tcatgcccgg accgttgtcg acgacctcga gccacacgcg cttcggcgac
     4681 gtgcccgtcc gcacgatgac cttgccaccg gcgtcggcga cggcatcgat cgcattgaac
     4741 agcacgttga cgagtgccgc gagcagctcg aacgaatcgc agtcgacccg cttcggccgg
     4801 ccctgatcgt aggccagctc gatctcttgc ttcggcgcga tgctgcgcgc gtatgcgcgt
     4861 gcgatctcgg tcgcgcgcca ggcgaggttt tcgacatcgg cgcgaatccg cgtgctcgca
     4921 tttcgcggct gcgtgaactg gagcatgcga tcgatcgcag cctggccgtc gtcgaggacg
     4981 tcgcgcatcg acccgatcgc tgcacgcacc ttctccggcg cctgcgcctc gagcccgcgc
     5041 tccatgcgct gcaaatgcag accgagcgga tggacgagca cgttcttcag atcgtggacg
     5101 accccgagcg cgatctggcc gagcgacagc aagcgctccg agctgccgag cgtggtcggc
     5161 tccgcatggc caatcgcgaa gatcttgcgt gcgtcagact ttgtcgtact cgtccagtgg
     5221 agcagccggt actggccgtc cttgctgcga actcgcgcgc caaaacgcgc agttccttcg
     5281 tgcgttaaat cggcctgcac gcgcgcgcgg tcatcgctgt ggacgagcaa gaagaacgcg
     5341 gcgcccacga catcgttgct cgcgtagcca agcacggtcg accacgcagc attcgccgca
     5401 tgaatcgtcc catccgtccc catcacacaa cacacgtctg gcgacagcgc gaaaaacgga
     5461 tctaggtcac ctggcaacaa cacccgccag cattgagcat gacgagggtc cggttgaaaa
     5521 tgaccgaaag ggaaggatca acgtctgtta ggcggtctaa gcccggcgcg atctgccatt
     5581 agtgcgagct gcgtgcgatt cgcgaggccg aacaggccga gcaggctgga gacgtgggcc
     5641 ttcaccgcgc gttcgccgat tctcagcttc ccaccgatcg ccgcgttgtc gagaccgacc
     5701 gcgagccagc gcatcacttc gcgctgacgc tcggtgatgc gcgaccagtg acgatgcgga
     5761 gctttctcgt caccattgcg cagcccgacg gcgatgccgg tgacggcgtt cgcgcgatcg
     5821 ataccgccca cttcgagcac gaccccgtcg tcagcggact gctggaccgg ggtcgcgagc
     5881 acgatcacgt gcagcgcggg cagctcggca tgcagcgaac gcacgagcga cagcgtgtct
     5941 tcgctgtcgt gatcgatatc gacgatcgca atgtcgacac gtcgtcgttt tgcggccgct
     6001 accagtttgt ctttggagct gagttgaaga acgggactga agccttggtc agcgagcctc
     6061 tgcgcgacca cttgcctgaa cagctcgtgg tcggagagga gcgcgattgc cggctttcgc
     6121 ggcggcggca tgggacgcgc atcgcagcaa tccatgcgcc tgtaatttgg ccccaaatgc
     6181 gggccaaacg cgcagaggtt gcgtctggag tgtatttcta cgcacctctt gcgcgatttg
     6241 ttggtcgagc aaaaatggct tcgacagtcc gaactccgga cgaagcgagt ctcgtgggtg
     6301 accgtggcgc cttcggtcaa atcgcgaagc gcgtctactg aagctcgcgc cgcacgatcg
     6361 tggcgccttc tgtcaaagcg cgaagtttct gaatcgcgac cacgcgcggc agctgcttga
     6421 gcccgcagtc ggtcgtgagc gtcacgcgct cgggctcgat gtgcttgagc acttttcgga
     6481 tgcgctcggc gacctgctcg ggatgttcga tctcgagcat gcggacgtcg atcacaccga
     6541 cgtgcacctt cttgtcgctc ggtagcttcc gcaacaggtc ggcgtcctcc atctcgcgag
     6601 cagcgaagtc gagcacgtac gcgtcgacct tgacgccgtg atagtgcggc agcacttcgc
     6661 gataaccgcc cgcggtcatg ccttcgagct tgttgcccca cgcgttgccc atgcagaagt
     6721 gccacgcgcg ttcgacgcct tggggaatgt ccgccatcgt gcgatcgacc gtgtcactga
     6781 cccaatccca atccttgtcg cccgacagat gcggcatcca cgcgccgagg tcctcgagct
     6841 ggatgtgctt gcagcccgct tcgacgagct ccttgatctc cgcgcggaac acatcggcaa
     6901 gcgcgcgcga caggtcgtag cgattcttca ccggccccga acggaaatac gcgagcgtcg
     6961 acagctgcac gggccctgca ccgacacacg ctttgatcgg acgctgcgtg tggcgctgcg
     7021 cccagttgta gaacagcgcc attcgcaccg gaccgcgttc gatcgcgcct tcgaccgcga
     7081 cgccgccggc ttcgtcgagc gcccagatgt ctcgaccctt cgctttcgca cgcgcaggat
     7141 gcggcagctt ctccgccgag aatcccttgg tgcgctcgag ccagtaccaa aggaacgagc
     7201 caatacccat gtgatagtcg tcgaaccaca tctggccgtc ggtgaggatg tcgagaccgg
     7261 cctcctcttg atctttgatc accacgcgcg tcgcgtcgtg aaacgcttcg gcgtgcgagg
     7321 cgaccttgaa cgcttcgagc acgtcgcggc ctgcgagctg ttgcgtgaac cacagcggcc
     7381 gcggatacga gccgaccatc gtcgtcggga ggaggtgcat aggtgcgaaa tactacgccc
     7441 gtggctggtc ggcgggtacg aacgacgctg tcatgaacgt gttacggctt cgtcagtaag
     7501 gccaatgtct tgcaactccg acggggatga ggtccgcgct cgcgtgtctc gccgcgctcg
     7561 tacctgcggt cacgcatgcg gaccctgcgc ccgatcgcga cggctcggtc tggacagatc
     7621 cagagcttgg cttcggtgcg ggactcgcgg gcctcggtta cgcgggctcg atcgcgtggg
     7681 cgcggcagcg cactggtgac caaggcgcac tgtggatccc ggtgctcggc ccgtggctcg
     7741 agctcgccaa ctcgtccgac tgtggcaacg gcgagatgtc ttgcccgcac ggcaacgcga
     7801 cgcgcagcgt gctgatcgtc agcggtctcg tgcagctcgc cggcgtgggt ctgatggcgc
     7861 atgcgctcac caagccgcac cacaagaaac ctcccgtcgt gatcgcgccg accatgacgg
     7921 ggatgggcgg cggcgtgcag attcgcggcc ggttctaata gagccggtgc aaatgcgtgc
     7981 cgcggtagta gtgaccgagg atctgctcgc tcgtcttgcc ggcatccgcc atgccgatcg
     8041 caccgagctg gcacatgccg acgccatgac cgaagccagc gccttcgaac acgaacgtgt
     8101 tgccgtcgcg cttgacgtcg aacagcgtcg acttgaggcc gccgagcaag cggcgaatgc
     8161 gcaggtcacc actgacgtcg acggtcgatt tctcgccggt gatcgtcagg atctggatgc
     8221 gccccgatac gccgcgctgc ttcgcggtga gcgccttcac gcgaccgagc tcgggatagt
     8281 cggtcgagat ccgcagcgtc atgtcgtcga cgctgatctt ctcggtccaa cggaacttgt
     8341 tgcgggcttt ctcgacgcgt ccgcaccaga agttcttcgg atcctcggcg aggaacgctt
     8401 ggatgctcgt caccttcgac atgccaccct tcggatcgtc gcggcggccg cgcagtgacg
     8461 gatccggatc gccgccccag atcgcttcgt tgtcctcgct gtgtccgccg cacgtcgcgc
     8521 tgtagcgaac gtcgacgagg ccgccgccat cgcgcaacag caccatgccg cgcgtcttct
     8581 cgaccgcctt cgtcgtgcgc gggtcttcct tgccggcgcc cgcgtagacc tggcactgct
     8641 gcgtcgagca cagcaggaac ggatcggtga ggttgcggcg gccgatcttc tggagcagct
     8701 cggtgcgcgc cgcgatcgcc tgcgcttcga gcgcggccgc cggtgcatcg ggatacatct
     8761 cggcgggcac gagcccggca agtagcttgt cctcggagac cgcgttcgca gcgagtagcg
     8821 agccgtcgtg atcgagcgtg acgtagacat tgccccagta gcggcgatcc tcgatgccgg
     8881 tttgcagctg cgagccgccg gtgccggtcg gtacgtccgc gaccgtcagc gtctcggtct
     8941 gcttcttggg ctggaaccac agcaccgatg cgttgcggat cgtcgtgctg ccgctcttcg
     9001 cgacgatcgt gccggccggc cggcgcacga gctcgtcgtg cacgagcgtc ttcacgccgt
     9061 acttcttcgc gtgctcggcg gcgcgtgcgg tgcccttgcc cacggcgacc ggatcgatcg
     9121 cgatgcgaac ctcgcgcgtg tcgatcaatt cgccgccggt gccgaacacc gtgccgatct
     9181 cgaacgagcg cggatcgaag ccgcgatcct tccagcgcgt gagtgcggtc gagatgccgg
     9241 tcgcatcatc cggcccgagc gtctcgacga cggtccactc ctggatcacg gcaggcttcg
     9301 cgccttcgac ggtcacggtc cagctgtcgc cgccgtcggc ctcgatcgcg ctgccgcccg
     9361 agccgtcggg gcgcaccacg acgccgcctt tcgcgcgcag cttgatgtcc ttcttgccgc
     9421 ccatgatctc gacggtgacc agcggcacgc cggtgtcggt gaacgtgaac cgcgtcgagt
     9481 agaggatgcg gagcttgtcg ctcgacgacg tgtcatcagc gcgggccccg gccggctgcg
     9541 cggtgagcaa ggcgagggcg acgaggccag ctcggaacat ggtcgaggct aggcacccgt
     9601 cgacggtggg agcaagaaaa cggcagtcag gtccgggtgc ggcctcgtgc tcgcagtgcg
     9661 gccggcgtca agttgtgctc gcgtcgcagc agcgcgctca tccgtgacgc actgccgagg
     9721 ccgacccgat cggcgatcgc atcgatcttc agctcggtac cgaacaggag gtcttcggcg
     9781 gccgccaacc gaacccggcg cagctcgtcg ctgaacgacg tcccgaggcg cttgagctcg
     9841 cgttgcagtg ttcgcgtcga catgttgagc gcggacgcgc attgctcgat ggtcgcgcca
     9901 accaacatgg tcgcgagcgc cgagcgcact cgcgtgagca gcacaccatc acgacgcgcc
     9961 tcggtggcgg ccgcctccgc cgcgacgtgt gcagcgcgtg cgcccggatg atcgaggacg
    10021 tgcaacgcgg cgtcgagtgt gtcgacgaag cgcaggaagt gggtcggtgc gagcgacggc
    10081 agcgctcctg cgagcagaat gccggacagc ccgccgggga ggatcaccac ctgacgctcg
    10141 atgcgcgccg accacgaggg caactgtgta cgcgcggtgg tggtgaacgc gaggagcgca
    10201 tcgacgtcca cgcgttcgac agctcgacag tccatcagca cgcgcccgtt cgatgcgagg
    10261 tcgggatgct cgacgaactc tgcgaccgaa agcatctcgc gtacgcatcg ctcgtctagc
    10321 gtgccccact ggatcgagcc ggcgagatcc ggtgcggcgc accagatcac gaacgtcgag
    10381 ccgaccacgc agtggcccag cggatgtgcc aggaactcgg cgacgctccg agcgacgatc
    10441 atcgcaacgt cacatcgcgg agcgtgtgcc catcacggtg tccatccaag gtcgcttcac
    10501 acaccagttg gcgtcctgat tgtgacccat gtgatgcgcg tagtgccacg gcagatgctc
    10561 gcgtgcccac tcgggttcga ggtgcgctcg gcggtgtttg cggtggtagt cgaattcaca
    10621 gaaccacacc gtgccgacga agaacggcgc gatcgggacg agcggcagca ggacgagcgc
    10681 gcccgcggcc agcgcgagga cttctttgct ctgggcattc cagcgcagca gcgagccgcg
    10741 gtaggcgtcg tcgaggaacg catggcggcg cgacgcgcga tgatgctcgt gccagtggaa
    10801 gctccagaag ctgttcttgc gcttcccgag cccgtgcagc acgtgcttgt ggaacaccca
    10861 ctccatcgcg ttcgcgaaca ccagaccgac ggggatgcca atcatgaagt tagcttagtc
    10921 agcacccgcg agcgccgtga cgatcgaata gcgccattcg aggtgacgat cgcgccgttg
    10981 cgctgtaacc agccgccaca ggcgacgtag cagtagctgg gacagccttg ccatgaacat
    11041 tcagccgagt acatccaacg gattggagat ctctccggtc acgcgtgcgc gagtggagct
    11101 gcatgtcacc gaccggatcg aggagttcgt gtccgatccg gtcggccgct gcgtactcgg
    11161 accgacctac ttgatctggt gcgccacgcc tgatctgcag ggggcgatca tctggagcac
    11221 gatcgacgag ctcgcgcttc gcaacatgat ggcgatcggc cgattcatcc tgcatccagc
    11281 gctcgccgcg cgtaggcggc tggtggtgga ttgccgcgac gtcacgcgcg tcggtgcaga
    11341 cgtgctgctt ggattcgcgg agctcgccca cgatcgagtg tcggtgtggg ccacgtcggt
    11401 cgaacgccag gcggttatcg ttcctggcgg tctcgccgga ctcctgatcg gtggcgcgct
    11461 accctccgtc ggcgcgttgc agccgctgcg gttctcgcac gacctcgagt ccgcgttggc
    11521 gttcgtcgat catccggcgg cccgggtggc tcacgagctg gccaacgaga tcgcggcctc
    11581 ccagcggggg aaggcgatct tgctcacgcg acttcacgca cagctagcac acgacctcac
    11641 ggatgcttcc gtggagagct ccgcgagtgc gctcgggctg ggcacgagga cgatgcaacg
    11701 cgcactggcc cggctcggca catcgttcag cgatgagctc cgccgttgtc gcgtcatcgc
    11761 agccgaggcc ttgctcctcc acagtgacct caagatcgag gccatcgcgc tgagggtggg
    11821 attcggcaac gcctcgcgaa tgagtgccac gttgaggcgg gtactgaacg tcaccgccag
    11881 cgggttgcgt gcacgcgcac gacactgagc gtcgacacat cgggttggcg cgatcgggat
    11941 agcgactggc gcatgatcga ttcgtcaccg acggatcgac cgcgtacaca gatctcgacc
    12001 atgatcatgt tgcagcgcct gctacgtcgg ttaccgaacg ggtgggcact cgtcccgctc
    12061 cgactcgtcg tcggattcgg attcgcctat catggctatg cgaagctcgc gcgcggcccc
    12121 gaacacttcg cgacgattct atcggcgatg ggagtcccgg gaccgcatac gatggcttgg
    12181 ctgaccacgt tcgtcgagct gttcggcggt gccgcgttga tgctcggcgc gttcgtggtt
    12241 cccgtctccg taccactgat catagtgatg gccacggcga tcttcggggt tcacgcgcag
    12301 tacggattct cgtcggtgcg attgaaggcg atcagcgacg ccggcgcaca attcggcccg
    12361 atcggttacg agctcgacct cgtctacatc gctgccctgg tggcactcgc gatcgcgggt
    12421 cccgaccgac tgtcgttgga tcggtggttg gccgtgcgcc gttcgcgccg cgcttgacgg
    12481 tcaagcgagc gcgacaaccg gggcacggat cgcgccaagg cgtggggtga tgcagacctt
    12541 ccgtggacgc atgctagatg cggctgtatg cacgatgtac agcgagacta cgcaccgctg
    12601 attctgttct atgcgggcga tcggcgtcac ctcttttcgc tgttccggct tgccgatgac
    12661 tcggtcgccg cgatcaatcg ctacgtcaac gccggtgttg tcctggttgc actcgacgtc
    12721 gacgaggttg tcggccacct tcagctcatc caaaccgatc cgtggcagat caacagcctc
    12781 gcggtcgttc catcgcaccg gcggcgcgcc atcggaagag cgctcgtcga gacagcggtc
    12841 aagcacgcgc gcggcgtcgg agtgcgaacg atcgaagtcg cgactgcggc agccgacctc
    12901 ggcgcgcttc gtttttatca acggctcggg ttccgcatga ctcggatcga acgcgatgcg
    12961 tttaccgagt ccgcgggcta cccggcagat gcaatggtcg acggcgtgcc ccttctcgat
    13021 cgcgtgtggc tcgagcgcgc gatctagcgt tggcgtaacc acgtcgtgtc agccgcgtat
    13081 tcgtggccag agcgaatgcc tcgctcactg caactggaga ctcacatgct gtcgaactct
    13141 tccgatcttg gtgctcggtt ttctacacgc gaagcggtcg atgcacgaat ccgacagaca
    13201 cacgtcctcg ggacggcagt gctgcgctac ggagtcgtgt tcttgctggt cacgatcggt
    13261 atggccaagt tcttcgcgtt cgaagcggag ggaatcaaac cgctcgtggc taacagccca
    13321 ctgctgtcat ggatgctcgg tgtcttcggg gtacgcggca ccgctgacgt gatcggcagt
    13381 atcgagatcg ttgccggcgt cgcgatggcg acccggcgtt tcgcgccggt gctgtcggcc
    13441 ctagggtcag cgattggcgt gctcatcttc ctgaccacgc tctcgttcct gttctcgaca
    13501 ccgggtgcgc tctcgctcaa acaccctgca agcgcgtttc tgatgaagga catcgtgctg
    13561 ctcggcgcgt gtatcgcgac cggctcggag gcgctgcgcg aggcccacgt gcgacgccgc
    13621 gtgtagcccg catgccgcaa cccatcgcca gcactccgtc gaatctccgg tgtggtatcc
    13681 cgatacccga gcctatgttc gtcgacatcg cgacagcact ccgcatcgag catgccgaag
    13741 caagcatgat gcgcgacatg gtgaacgcgc tcgtcggaac gtcccgcgca ccagcggcat
    13801 tcgtgcgcga tctcggcatg ggcacggcca cgtacgtccg agccagctcg ccgctgaata
    13861 aagtgatcgg cgcgggcatc gatgcgccga tcgatgcggc gctgctcggc gcgaccgaac
    13921 gtgcattcga tagcaaaggc gaacccgttc gcgtcgagct atcgaccctg gctgatccgc
    13981 agacgtatcc gctgctcgcg gagcgcggct atcggctgct cggcttcgag aacgtgctcg
    14041 cgcggtcact cgtcggcgtc ggtcccacgc cggtcgcgaa cgttcgcatc gaaacggtga
    14101 ccgcgagcgt actgtcggaa tttcgcgaca cgacgatcga ggccgctgct catcccgacg
    14161 atacgggcgt cgtgctcgac acgttctcgc gtgacgtgat cgctacggcg gttgacgatt
    14221 cgcttggcag ccacggtttc acgcgctacc tcgcctaccg tgacggcgcg atcgcgggtg
    14281 ctgccagcat gacgatgcat ggtgacgttg ctgtgctggc gggatccggg acgctcgtag
    14341 cacaccgacg gcgtggcgtt caggcggccc tgctcgcgac gcgcctcgcg gacgcatgcg
    14401 cggccggcgc tacgcttgcc gtcatcacca cggcggccgg tacccagtcg caggcgaacg
    14461 tgatgaagct aggtttcgcg ttggcctacg cccgcgccgt gctcgtacgt gcgccacgcc
    14521 cggcggcgtg atgtcacgca tctagtagct cggtcgcgag tgcgagcgtg cgtgcgttct
    14581 cgggcaagtt cgtgagaaag ctcgtgcgcc actcggcgtc ggtgatcttc gtcgcgcggg
    14641 cgagtacgcg ttcgcgcgca cgccggatcg cttcgcgggc accctcggcg tcgcccgagg
    14701 cgcgcagtgc ttctgcgacc atcagccgga tctgcgactc gccttcctcg acgccgccga
    14761 gcatctcgag caggtcgtgc gcttcgcgcg cggcgatcaa cgcctcgtga tggcggccct
    14821 gtttgagcag catgctcgcg aggtcggcga gcagggcagg gcgtgacgcc gcctgcacgg
    14881 tcttgagcgc ctggcgcacg agctcttcgg caacgctgag ctcgccgcgc atcgcgagga
    14941 tgctcgcgag gtaggtgcgc gatgccgatt cgagccggcg atcgccttgc gcggcgaacg
    15001 cttcgaccgc ttcggtttcg atcgccgccg cctcgtcgag cttgccctgg tgctggagcg
    15061 cgcggccgag gttgtgcttt gcggtcgcga cgacgttttg caggcccatg cggagcccgc
    15121 cgtcgagcgc cttgcgcagc cagcgttcgg cttcggcgta ggcgccgatt tcgagatacg
    15181 cgtagccgac gtggccgtgc tgcatgcacg cgtagcgcag atcgccggcc tgctcgaacg
    15241 cgagcgcggc ggcttgctcg gagacgacgt aggcgccgga gtcaccggag aacatcgcgc
    15301 gtgacgatcg cgtctgatag atgcgggcga ggatgcgcgg gtcgtggacc tcggcggcga
    15361 cgcgatcgat ctgatcgacc atctccgacg ccagctggta cttaccgtgg ttgtacagct
    15421 ggaacgcgca ggtcgcggtc gcggtgacgc gcgctgcgag ctcgccttcg agatcgggcg
    15481 cttcggtgag cgctcgcgcg atcgtcgcga gctggtcgag gtcgccgagc ttgccgcacg
    15541 cttcggcagc ctcgccggct gcggcgaacc acggcgccga cccgcgcagc aagagctcca
    15601 tcgcctgcag cgagtagcgc tccgcctcga cgaactcgcc gcaccacttg tgcgcttcgc
    15661 cttgggcacg gcgcaagagg ccgagtgtct cgcccgacgc accgcatgcc acgccgcgct
    15721 cggcgcgctg agcggctgca cgaaagtcgc cggcggcgag tgcttggtcg acggcggggc
    15781 caaaccacag cgccgcgcga acgggtgccc cgccgcgctc gtagtgctcg gcgagcacca
    15841 tcgcttcggt ctcgcccgca tactgcaacc actcgccggc gaggcgatgg ccgagcttgc
    15901 gatcgctgtc ggtgagcatc gcgtacgcgg cctcgcgcac gatcgcgtgg cggaacagat
    15961 actcgtcctc gtcggtaaag cgcgagcccg gagccatcgc gatcagctcg cgctcgacca
    16021 gctcgtcgag ccacgcgcgt accgacgagt cacgcgcttc gccaccgagc agcttcgcga
    16081 cgccgccgcg ccagaactgc gcgccgtaca cgctcgccgc gcgcagcacg cgccgcgcat
    16141 cgacctccag gcgctcgagc cgaccttgga cgacggcgag cacggtctcc ggcatcgcgt
    16201 cgcctttgcc ttccgcgacg gcgcggatca gctcttcgag atagaacgcg ttgccgccgg
    16261 cctgttcgac gagtcgcgcg accagcgcgt cgttggcgcg atcgccgagc acctgacgca
    16321 cgagcttttc gctgccgcgc ttcgtcagct cgtcgaggcg caggtcatgg acgccgcggt
    16381 ctttccataa cagcggaaac atctcctgga tctcgggacg cgcgaacgcg gcgacgaacc
    16441 agggccggtc cggcaacgca cgcaacgccg attcgatcag cttcgtcgtc ggcaaatcac
    16501 cccagtgcag atcctcgagc acgatcacga ccggctgcgc ggcggtctcg atcgcgagaa
    16561 agtgctcgaa ggcgcggcgc acctgatcag acatcagcaa cgcgtcctgg cgcgcggcgc
    16621 gcagctcgac cgagtcgtcg tcgggaaatg gaacgccgac gagctcgccg agaaacatca
    16681 cggtacgcgt aggctcggca ctcgcatttc gcatcacgcg cgcgcgcaac ttctggcgcc
    16741 ggacttcgac gggttcgcca tcgagaatgc ctgctgcgcg gcgcagcgcg ggcgcgatca
    16801 tgccgaacgg cgaaccggtg tgcagcggat caccacgacc gatccagatc tcgaccggct
    16861 cctcgcgcgc cttgatccgg cgcagcagct cgtagcgcac gcgcgacttg ccgatgcccg
    16921 caacgccggt gacgagcact gcgcgcgcca tcggttcggc gaccgcctct tcgaacaagc
    16981 ccatcagcac gccgagctcg cgatcgcg
//
LOCUS       DASUFX010000277         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1572723, whole genome shotgun sequence.
ACCESSION   DASUFX010000277 DASUFX010000000
VERSION     DASUFX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572723"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFV99_11030"
     CDS             complement(<1..528)
                     /locus_tag="VFV99_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059886.1"
                     /translation="MDAAAQLEVIEQLFFSDRRQAMEKLSDFVEAAKGKKRAPAMAEL
                     VKRVDQEHPGLAAYLALAGGAIVEDGEEAGALGRALVKPLERSLVAAARMVGHVAHLP
                     DQDMGDDDDENEEAENDDEAHAHAHGHDHDHDHDHDHDHHDHDHDHDHDHHHHNGNGH
                     AHSHEEEEHVMIGTKA"
     gene            complement(540..1961)
                     /locus_tag="VFV99_11035"
     CDS             complement(540..1961)
                     /locus_tag="VFV99_11035"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HEX5059887.1"
                     /translation="MRRFALLVLFAACGDNLDTTPTETPVEAVCNENDLDSLVATLPN
                     VTSAKAVTCGDFVQGAVRCYEVTITQPVNQVTPGATFGQHLFLMHRGCDRPTVVADWG
                     YSNDVFFDDELAVLFDANALWIEHRYQGSSAPVGADWDWTQLTIKNGATDMHRVIDSF
                     KHLYGANWVSTGASKGGITATYHSFFFPNDLDGRIPYVAPASRSRIDSNYQTYLDRTL
                     TSDCAQRIRDAQIAALTTRRSMMLSHLAQTVGAGNEEIYLDAMTASFDWAFWQYRGER
                     YCNSVPPATATDDAFWTFYAQTSGFFGPADDSLYADGALSYEWLSEQGFALQIGAHVR
                     DLLTSEYATMTMEDRFRSDFPDVDLPIYDGAVTRSARHWALHYAQNMLLIYGQFDPWS
                     GGALDEPEQPTSARLFAPAQTHGGAAIATLTDADQALAIEHATRMFGVPPIMTRMRLA
                     AAATERRDAILARHEQRVLLRLP"
BASE COUNT          391 a          735 c          761 g          418 t
ORIGIN      
        1 cgccttggtg ccgatcatca cgtgctcttc ctcttcgtga gaatgcgcgt gaccgttgcc
       61 gttgtgatgg tgatggtcgt gatcatgatc atgatcgtgg tcgtgatgat cgtggtcatg
      121 atcatggtcg tggtcatggt cgtgcccatg agcgtgtgcg tgcgcctcgt cgtcgttctc
      181 ggcctcttcg ttctcgtcgt cgtcgtcgcc catgtcctgg tccggcagat gggcgacatg
      241 cccgaccatg cgtgcggcag cgacgagcga gcgctcgagc ggcttcacga gcgcgcgccc
      301 gagcgcaccg gcttcttccc cgtcctcgac gatcgcgccg ccggcaagcg cgaggtacgc
      361 ggcgagcccc ggatgctcct gatcgacgcg cttgaccagc tcggccatcg ccggagcccg
      421 cttcttgccc ttcgctgctt cgacgaagtc ggagagcttc tccatcgcct ggcggcgatc
      481 actgaagaac agctgttcga tgacttcgag ctgcgcggcg gcgtccatac ccgcgcagtc
      541 tacggcagac gcaacaacac tcgctgctcg tggcgcgcaa gaatcgcgtc gcggcgctcg
      601 gtcgccgctg ccgctagccg catccgggtc atgatcggtg gcacgccgaa catgcgcgtc
      661 gcgtgctcga tcgcgagcgc ctgatcggca tcggtgagcg tcgcgatcgc ggcaccgccg
      721 tgcgtctgcg ccggggcgaa caagcgcgcc gacgtcggct gctccggctc gtcgagggcg
      781 ccgccactcc acggatcgaa ctggccgtag atgagaagca tgttctgcgc atagtgcaac
      841 gcccagtgcc gcgccgagcg cgtgacggcg ccgtcgtaga tcggcaagtc gacgtccggg
      901 aagtcggagc ggaagcgatc ctccatcgtc atcgtcgcgt actcgctcgt cagcaggtca
      961 cgcacgtgcg cgccgatctg gagtgcgaac ccctgctcgc tgagccactc gtagctgagc
     1021 gcgccatcgg cgtacagtga atcgtcggcg gggccgaaga agcctgacgt ctgcgcatag
     1081 aacgtccaga acgcgtcgtc ggtcgccgtc gcgggtggca ccgagttgca gtagcgctcg
     1141 ccgcggtact gccagaacgc ccagtcgaac gacgcggtca tcgcgtcgag atagatctcc
     1201 tcgttgcccg cgccgaccgt ctgcgcgaga tggctcagca tcatcgaacg gcgcgtcgtc
     1261 agcgcggcga tctgcgcgtc acggatgcgc tgcgcgcagt ccgacgtcag tgtgcggtcg
     1321 aggtacgtct ggtagttcga atcgatgcgg ctgcgcgacg cgggcgcgac gtacggaatg
     1381 cggccgtcga gatcgttcgg aaaaaagaac gaatgatacg tcgcggtgat gccgcccttg
     1441 ctcgcgccgg tcgacaccca gttcgcgcca tagaggtgct tgaacgagtc gatcacgcga
     1501 tgcatgtcgg tcgcgccgtt cttgatcgtc agctgcgtcc agtcccagtc agcgccgacg
     1561 ggagccgagc tcccctggta gcgatgttcg atccagagcg cgttagcgtc gaacagaacg
     1621 gcgagctcgt cgtcgaagaa gacgtcgttc gaatagcccc agtcggcgac gacggtcggc
     1681 cgatcgcagc cgcgatgcat gaggaacagg tgttgcccga acgtcgcgcc cggggtcacc
     1741 tggtttaccg gctgcgtgat cgtcacttca tagcagcgca ccgcgccttg cacgaagtcg
     1801 ccacacgtga cggccttcgc cgaagtcacg tttggcagcg tcgcgacgag cgagtcgaga
     1861 tcgttctcgt tgcaaaccgc ttcgaccggc gtctcggtcg gcgtggtgtc gaggttgtcg
     1921 ccgcaggcag cgaacagaac gagaagcgcg aatcgtcgca tgagccgacg ctatgacagc
     1981 tgaagtcgcc ttgggttgct tggacttctg acagccccac tcgcgcagcg actgacagac
     2041 tgcgtcgctg ttacgtcggt gcgacacctg tcaggtcgcg cgggcaagtg cgcatcgtga
     2101 ctggaacgtg cgctgcatct cttcgacgca gaattaggag gatgacatgc gaaagcttca
     2161 attactgtcc ggcgcgctcg cgctcgggct cttgatgaca ggcggcgcct gcaagcgcca
     2221 ggagtcgcca ccacggcgcg acaccacgac gcaagaagag ccgtcggctc gaaaagaggt
     2281 cgagcaagcg caacgcgact tagag
//
LOCUS       DASUFX010000278        11954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1572764, whole genome shotgun sequence.
ACCESSION   DASUFX010000278 DASUFX010000000
VERSION     DASUFX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572764"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFV99_11040"
     CDS             complement(<1..196)
                     /locus_tag="VFV99_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059888.1"
                     /translation="MGRSVGRKKHLQLSLEDARKETGRGGWRRNAGRKKKPGAISHDA
                     RHELTSYHPQHVTLRLVDDAP"
     gene            complement(290..580)
                     /locus_tag="VFV99_11045"
     CDS             complement(290..580)
                     /locus_tag="VFV99_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059889.1"
                     /translation="MERHPDTEPFGVLRFDDVVQHELGAPSEDRLRHHPLWGKGLDYY
                     SFHMLEAVDGRTRWLVTFHDETLDVTAMAAKASPLIFASNHLAAIENVRRAT"
     gene            complement(779..3058)
                     /locus_tag="VFV99_11050"
     CDS             complement(779..3058)
                     /locus_tag="VFV99_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059890.1"
                     /translation="MNPVADPSPEQFGPYEVYERLGLGGMATVYRAKKRGPAGFERSV
                     ALKRMLQHLAEDPAFVESFVREAKVASLLQHPNIAQVYDFGRISGVYYIAMELVAGFD
                     VRKLLRYANRANEAIPMPVILSILGELCDALDYAHTFVDENGQPLHIVHRDVSPSNMI
                     MAHTGHLKVIDFGIAKANSRQLYTESGQVKGKLGYMSPEVALGMPVGPVSDVFSVGVV
                     AWELVTASPLFSARSDFETMRKIREQPIAPPSRHNPDCPPKLDQLIAAALQREPENRL
                     ASARMFRTALDQIAVETGSHVSARAVAEWMLKFVQPDDPWARAAAASLQASSTARNRS
                     PSGSRSLPLPPEPPTAVMRGTHSRPVPLRRSAEDIQLASEIWGEDVATIGDGAAPAPD
                     FHVQVASTPAPAELTPAHVRPPTQRPPTQRPPTQRPPTQPPPPSALRVPSQQRPPSHV
                     HPVQPRSSKKPLLALGALALVAAALAGVLIWKRQSAGPTNAALRFEIEPAGAVLTLAG
                     KRIGDTSPLETQLAAGTYPVIVELDGYEPYKTTLTVREGEHQTIHVALDKADTAIAIE
                     QPPPPPPPPPVQEPPKQVAVEAPAEEEPPKQPTHESKRPATKQTKKGKKEKVVAEAPP
                     KEERKEDPPKEDPPKVDPPKVDPPKEDPPKIDPPKVDPPKPTRALGVAATAVTKLSGD
                     VPTLRAKSAEGGGNVSAKLCIDESGHVSSVKIVKGNLEIASQLEDALSSWRYKPFLRD
                     GKAIPVCFLLSFPVVVKGG"
     gene            complement(3125..5884)
                     /locus_tag="VFV99_11055"
     CDS             complement(3125..5884)
                     /locus_tag="VFV99_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5059891.1"
                     /translation="MRVTRLGLVVVVLWTGSASAQPEGEAADEETKPAPEESAEKAPE
                     PPPATTGTIEGTVEAPDLDAPLAGATVTIVGSGITATTDDEGHFKLENVKPGSVKVRA
                     DFGGFSSLEQTIKVEVGATSKLDFQLATIPLLNETIVVVGSRTPRTNVETTVPVDVVT
                     AEEMSRSGRTETGRVLHTLAASYISTPQSIADGTDHVDPASLRGLGPDQVLILINGKR
                     RHRSALLHVNGTFGRGTVGTDLNAIPAGSIKRIEILRDGAAAQYGSDAIAGVINIVTK
                     DVTDLLDVTAETGITAAGDGAQLKASANYGFKIGQKGFVNVTGEYLERQATDRSGTYT
                     GTIYSDDPAIEQQMLGNKSRDLFKMGIGEAAAIAGMGSYNLELPINDVATFYSFGDLS
                     HRRGAANGFYRFPKQVSQNVPEFYPNGFLPEIHPTIDDRAITIGMRRKGTWDVDASLT
                     HGESSFQFNIEESVNASLGTSSPTTFDAGTLSFSETLANLDLLRKLDVDGIKSLALVL
                     GSELRVERYQIEAGDEASYSLGPATYGTPPMPKLPGAQVFPGFQPSNEVDRTRDNIGL
                     YGGIESELNKAIALDAGGRFETYSDFGRSLIGKVAARAKLGGPFAIRGAASTGFRAPS
                     LQQLWFSNISTLFLADMTGTLQPTQVLTSNNASPVTKAFNIPRLREERSINFSGGITV
                     HLRDTLSMSVDGYFIRLRDRIVLTSQFTNTNPEVAAILAQFPGVSQAQFFANAVDTDT
                     KGIDVVFDYNVDAAGGQLAFTAAANFTRTDVTNVNIPEELKNKFTDAAQLRTFFFGRL
                     AENRLEDSVPHQKGSAAVRYSINGVSALLRGNYYGKVYYRPDNAMNDEVFGAKVLVDA
                     DLGYQVTKNLLISVGADNMFNTFPDPQQKDANISFGRFIYSRNVSQFGMNGAFYYGKL
                     QLTFF"
     gene            complement(5921..6997)
                     /locus_tag="VFV99_11060"
     CDS             complement(5921..6997)
                     /locus_tag="VFV99_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059892.1"
                     /translation="MMRRLVLVVAIVAAGCSRDTIEVNKKPSDDYKHAVVVGAVDKFV
                     AAGRTPDAFAEMAQTLQAARPQMDRTVAREAELKLVTLAIHPIQQLQAKSMREKIDAL
                     ALTVWPSLLGPSITADKQLEVKDPKAPEIVPKPGEDPDQYLVRLCGGPLARECKSVVP
                     ELQGDVIEALALRRGMERARNAVTECLACRDDDAWKQTVRAWEKLDAETAETISNIER
                     QADPDNWPVAGAAADDDPSLPEAEFSTRGDLIVGGHSYGPNQQRIQVLEELRGNGEVL
                     ALHFHPDTTLAQMRALLVDARKAGAKRVAVIAREPFYPYARKAYWVADGYGLRANLRP
                     TDSLQLLLHAIDEVAGPGTVARVD"
     gene            complement(7048..8010)
                     /locus_tag="VFV99_11065"
     CDS             complement(7048..8010)
                     /locus_tag="VFV99_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5059893.1"
                     /translation="MRVPLVILTGFLGAGKTTLLNRLLADRAARNAHGKIGVIVNELG
                     EVGIDGALLGTEAARQIELPGGCVCCVLGDELDKTVIDLVTANPDLEAIVLETTGVAE
                     PLPIAWALAREPVRDYVRLAIVVTLLDATNFRASRGVSVAVAAQVAYADVLLVTKESL
                     AGDADTKATIEAAHVLAPSAQILRGTTEEHVTWLERVLADPDVEHVPTGEPHVHDENC
                     RHPDSHAHGVDSTWLNVEARIDLEELEDQLAALPANYVRIKGIVRTGDGSWSAIHRVG
                     LRVSSEPLDRQPDDIVGENGRLVALGTQLDPEKLAICLGTAYVK"
     gene            complement(8034..9419)
                     /locus_tag="VFV99_11070"
     CDS             complement(8034..9419)
                     /locus_tag="VFV99_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059894.1"
                     /translation="MRDLYGQLLEKALFPAFEAARGRPTVPLLRYLEETSHWSLPELH
                     GLQTGFLRRLLRHAYRHTAHYRRVLDERGLRPEDFASPVDLQKLPLLDRPTLRATMME
                     RTANAPPAAVIRKVTSGSSGQPVEVLYNAESRHWRDAIRLRGYGWGGYGIGKRSFHYW
                     GFVPPAGTTWWKRTKTELDRRVKREVFLDCTPRGEPALLEAVDYVRRYKPQCIVAYAS
                     GAGALAHFINARNLRDWSDIPVLTGAEGLLPHDRAAINRAFGPAFDTYGCREVMLMAS
                     ECEAHEGLHTSMENLIVEVVVREGNTVRPARPGEVGEVVITDLHNLACPMIRYVNGDL
                     AVAGVEERCRCGRGLSRIGQIQGRITETMYDGQGRAVGGLVFNILFSTIGHVARSFQV
                     HQHEDGRVVFKVVPYQGGGLPWREEQLLRSHAERYLPGAPFTIEIVDNIPLSPAGKRR
                     VVVCEYKARAS"
     gene            complement(9416..10792)
                     /locus_tag="VFV99_11075"
     CDS             complement(9416..10792)
                     /locus_tag="VFV99_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059895.1"
                     /translation="MKDLYGPLLAKALFPAFEAARGRPTVPLLRYLQTTERWSLPALH
                     DLQVGFLRRLLRHAYQHTAYYREVMDERGIRPEDVVRVEQLAELPLLDRDTVRATMDS
                     RLSGAPPRVAIKKSTSGTTGEPVVVKYNEESRHWRDATRWRGYGWGGYCIGMRALHYW
                     GFGPPSKSWMHRRKVWLDRAFKRDLYVDCTPRGEAALMGAVKTLRSYQPQVMVAYSAG
                     AAALARFVNDNKLRTWRDFPVLVGAERLWPHDREAIVEAFGPAFETYGCRETMLVASE
                     CEHHNGMHTSMETMVTEIVVRLPDGSDRPARPGETGEVVVTDLHNLACPMIRYLNGDL
                     AVAHAPSECTCGRSLTRIGPIEGRVTETLRDGRGNPVGGLVFNILFGVMDQVAKKFQV
                     VQRLDGSVVMKVVPQIGSRLPDENHRAIHAFADKYLPDAPFAIEYVEDIPLTEAGKRK
                     VVIVEKPA"
     gene            10821..11909
                     /locus_tag="VFV99_11080"
     CDS             10821..11909
                     /locus_tag="VFV99_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059896.1"
                     /translation="MLSRLRKKNLHQWLPDYARHLVRRARTPQSNAPRHILFALCDHY
                     EPLHGRAPDDIGRTRVDAWVDGYPKLREFRDSNGRPPRHGWFFPGEEYRPHYLDGLAK
                     LARAGFGEVEFHLHHDGDTAHSLAPRIKDHLDTFAQHGHLSRRGDAYQWAFIHGNWSL
                     ANGRPDGKWCGVDDELVMLHELGCYVDLTFPSAPDPCQPDKVNQIYWPIGDLTKRRSY
                     AQGERARVGASYDDRLLMITGPLAFARKGLGVRLENGALTGNDPPTAARVDTWISQGI
                     HIEGRPEWTFVKVHTHGAIEKTASSLLGEGSRVMHAALQRYMREGWKLHYVTAREMYN
                     VARAAMDGVLGEPSDFFDYIVKPPPIAS"
BASE COUNT         1962 a         3923 c         3881 g         2188 t
ORIGIN      
        1 tcggtgcgtc gtcgacgagg cgcagcgtca cgtgctgcgg gtgatacgac gtgagctcgt
       61 gacgcgcgtc gtgcgagatt gcgcccggct tcttcttgcg ccccgcgttg cgccgccaac
      121 cgcctcgccc cgtctccttg cgcgcgtctt cgagactcag ttgcagatgc ttcttgcgtc
      181 caacactccg acccatgagt tgttggtagc aaaccggtct gacatcgatt tctcgtcgag
      241 aaatgcgtgc gaatgatttt cgcactgatg tcgtgcaggg gattcggcgc tacgtagctc
      301 gccgcacatt ctcgatcgcg gcgaggtggt tcgacgcgaa gatcaacggc gacgctttcg
      361 ctgccatcgc ggtgacgtcc aacgtctcgt catgaaacgt aacgagccag cgtgttcgac
      421 catcgacggc ttcgagcatg tggaacgagt agtagtccag ccccttgccc cacagtgggt
      481 ggtgccgaag ccgatcttcg ctgggtgcgc cgagctcgtg ttgcacgacg tcgtcgaatc
      541 gcaggacgcc gaatggctcg gtgtcggggt ggcgctccaa atacgtgtca gtctcgtgat
      601 gtccccatcc gggaaattcc gggtcgtggg cgcggtatgc gacccagagc gtgcgcccct
      661 cgacgagcgt ggtcggctct ggcgcgccgt gtccatcggc accggctcca tccaaatttg
      721 aagcgatgtc gtcacgtcaa atgatttccg cactgatcgg aacaaccagc cgtggcgatc
      781 aaccgccctt gaccacgacc gggaacgaca gcagaaagca caccgggatc gccttgccgt
      841 cgcgcaggaa cggcttgtag cgccacgacg acagcgcgtc ctctagttgg ctcgcaatct
      901 cgaggttccc tttgacgatc ttcaccgacg acacgtgacc cgactcgtcg atgcaaagct
      961 tcgcgcttac gttgccgcca ccctccgcac tcttcgcccg cagcgtcggt acgtcgcccg
     1021 acagcttcgt caccgcagtc gcggcgacgc cgagtgcgcg cgtcggcttc ggcggatcga
     1081 ccttcggcgg atcgattttc ggcggatcct ccttcggcgg atcgactttc ggcggatcga
     1141 ccttcggcgg atcctccttc ggcggatcct ccttccgctc ttctttcggc ggcgcctcgg
     1201 cgacgacctt ctccttcttg cccttcttcg tctgcttcgt cgccgggcgc ttcgactcgt
     1261 gagtcggctg cttgggcggc tcttcctcgg ccggtgcctc caccgcgacc tgcttcggcg
     1321 gctcctgcac gggcggcggc ggcggcggtg gcggcggctg ctcgatcgcg atcgcggtgt
     1381 ccgctttgtc gagcgcgacg tggatcgtct ggtgctcacc ttcgcgcacg gtgagcgtgg
     1441 tcttgtaggg ctcgtagccg tcgagctcga cgatcaccgg atacgtgccc gcggcaagct
     1501 gcgtctccag cggcgaggtg tcgccgatcc gcttccccgc gagcgtgagt actgcgcccg
     1561 cgggctcgat ctcgaatcgg agcgccgcgt tcgtcgggcc cgccgactga cgcttccaga
     1621 tcagcacgcc cgcgagcgca gccgcgacca gcgcgagcgc accgagtgcg agcagcggct
     1681 tcttgctcga gcgcggttga acgggatgga cgtgcgacgg cggtcgttgc tgcgacggca
     1741 ctcgcagcgc ggacggcggc ggaggttgcg tcggaggacg ttgcgtcggg ggacgttgcg
     1801 tcggcggacg ctgcgtcggc ggccgtacgt gcgcgggcgt cagctcggct ggcgcgggcg
     1861 tcgaggcgac ctggacgtgg aagtcggggg ccggcgccgc tccatcgccg attgtcgcga
     1921 cgtcctcgcc ccagatctcg ctcgcgagct ggatgtcttc ggccgagcgg cgcagcggaa
     1981 cggggcgcga atgggtaccg cgcatcacgg cggtcggtgg ttccggtggc aacggcagcg
     2041 aacgcgagcc ggaaggcgac cgattgcgcg ccgtcgacga tgcttgcagg cttgctgcgg
     2101 cagcgcgcgc ccacgggtcg tcgggctgca cgaacttgag catccactcg gccaccgcac
     2161 gcgcggagac gtgcgatcca gtctcgacgg cgatctgatc gagcgcggtg cggaacatgc
     2221 gcgcgctcgc aagccggttc tcgggttcgc gctggagcgc ggccgcgatc agctggtcga
     2281 gcttgggtgg gcagtctggg ttgtggcgcg atggcggcgc gatcggctgt tcgcgaatct
     2341 tgcgcatcgt ctcgaagtcg ctgcgcgcgg agaacagtgg cgaggccgtg acgagctccc
     2401 atgccacgac accgacggaa aagacgtcgg agaccgggcc gaccggcatg ccgagcgcga
     2461 cctcgggaga catgtagccg agcttgcctt tgacctggcc ggactcggtg tagagctggc
     2521 ggctgttcgc ttttgcgatg ccgaagtcga tcaccttgag gtgaccggtg tgcgccatga
     2581 tcatgttgga gggcgacacg tcgcggtgca cgatgtggag cggttggccg ttttcgtcga
     2641 cgaacgtatg cgcgtagtcg agcgcgtcgc acagctcgcc gaggatcgag aggatcaccg
     2701 gcatcgggat cgcttcgttc gcgcggttcg cgtaacgcag gagcttgcgg acgtcgaagc
     2761 ccgcgacgag ctccatcgcg atgtaataga cgccgctgat gcgaccgaag tcgtagacct
     2821 gcgcgatgtt cgggtgctgg agcagcgagg cgacctttgc ttcgcgcacg aacgattcga
     2881 cgaacgccgg atcttcggcg aggtgctgca gcatgcgctt gagcgcaacg ctgcgctcga
     2941 agccggcggg cccgcgcttc ttggcgcgat agaccgtggc catgccaccg agcccgagcc
     3001 gctcgtagac ctcgtaaggt ccgaactgct cgggtgatgg atcggccacc gggttcacca
     3061 ctgctccact aactccatgc ccagcatgag ttgctgagag tagccgatca agtcgcgcct
     3121 gctctcagaa gaacgtcagc tgcagcttgc cgtagtagaa ggcgccgttc atgccgaact
     3181 ggctgacatt gcggctgtaa atgaaccgac caaagctgat attggcgtct ttttgctgcg
     3241 ggtctggaaa cgtattgaac atgttatcgg cgcctaccga gatgagcaga ttcttcgtga
     3301 cctgataccc gaggtcagca tcgacgagaa ccttggcgcc gaacacctcg tcgttcatcg
     3361 cgttatccgg acggtagtag accttgccgt agtagttccc gcgtagcagc gccgacacac
     3421 cgttgatgga atagcgaacc gcggccgagc ctttctggtg cggcaccgaa tcctccaggc
     3481 gattctcggc gagccggccg aagaagaacg tgcgcagctg agcggcatcg gtgaacttgt
     3541 tcttgagctc ctctggaatg ttcacgttcg tgacatcggt gcgcgtgaag tttgcggccg
     3601 cggtgaacgc gagctgaccg ccggcggcat cgacgttgta atcgaacacg acgtcgatgc
     3661 ccttcgtgtc ggtgtcgacc gcgttcgcga agaactgcgc ctgactgacg cccgggaact
     3721 gcgcgaggat cgctgcgacc tcgggattgg tgttcgtgaa ctggctcgtg agcacgatgc
     3781 gatcgcgcag gcggatgaag tagccgtcga cgctcatcga cagcgtgtcg cgcaggtgaa
     3841 cagtgatgcc gccgctgaaa ttgatcgagc gttcctcgcg cagcctggga atgttgaatg
     3901 ccttggtgac cgggctcgcg ttgttgctcg tcagcacctg cgtcggctgc agcgtgccgg
     3961 tcatgtcggc gaggaacagc gtcgaaatgt tcgagaacca cagctgctgc agcgacggcg
     4021 cgcggaagcc ggtgctcgcc gcaccgcgaa tcgcgaacgg accgccgagc ttcgcgcgtg
     4081 ccgcgacctt gccgatcagc gagcggccaa agtcactgta cgtctcgaag cggcctccgg
     4141 cgtccaacgc gattgccttg ttgagctcgc tctcgatacc gccatagagg ccgatattgt
     4201 cgcgcgtgcg atcgacctcg ttgcttggct gaaagccagg gaagacctgc gcgccaggca
     4261 gcttcggcat cgggggcgtg ccgtacgtcg cagggccgag gctgtacgac gcttcgtcgc
     4321 ccgcctcgat ctgatagcgc tcgacgcgca gctcgctgcc gagcacgagc gcgagtgact
     4381 tgatgccgtc gacgtcgagc ttgcgcaaga ggtcgaggtt cgcgagcgtc tcggagaaac
     4441 tgagcgtgcc ggcgtcgaac gtcgtcgggc tcgacgtgcc gagcgatgcg ttcaccgact
     4501 cctcgatgtt gaactgaaac gagctctcgc cgtgggtgag gctcgcgtcg acgtcccacg
     4561 tgcccttgcg ccgcatgccg atcgtgatcg cgcggtcgtc gatcgtcgga tgaatctcgg
     4621 gcagaaatcc attcggatag aactcgggca cgttctgcga gacctgcttc ggaaaccgat
     4681 agaagccatt cgccgcgccg cggcgatgcg agagatcgcc gaagctgtag aacgtcgcga
     4741 cgtcattgat cggcagctcg agattgtacg accccatacc cgcgatcgca gccgcctcgc
     4801 cgatgcccat cttgaagagg tcgcgggact tgttgccaag catctgctgc tcgatcgcgg
     4861 ggtcgtcgct gtagatcgtg ccggtgtacg tgcccgagcg atcggtcgct tggcgctcga
     4921 ggtattcgcc ggtgacgttg acgaagccct tctgaccgat cttgaagccg tagttcgcgc
     4981 tcgccttgag ctgcgcgccg tcacccgccg cggtgatgcc ggtctcggcg gtcacgtcga
     5041 gcagatcggt gacgtccttc gtcacgatgt tgatcacgcc cgcgatcgcg tcggagccgt
     5101 actgcgccgc tgcgccgtcg cgcaggatct cgatgcgctt gatcgagccg gcgggaatcg
     5161 cgttgagatc ggtaccgacg gtgccgcgac cgaacgtgcc gttgacatgg agcagcgcgc
     5221 tgcgatggcg gcgcttgccg ttgatcagga tcagcacctg atcgggcccg aggccgcgca
     5281 agctcgccgg atcgacgtga tcggtgccat ccgcgatcga ctgcggagtc gagatgtagc
     5341 tcgcggcgag cgtgtgcagc acgcggccgg tctcggtgcg gccgctgcgc gacatctctt
     5401 cggccgtgac aacatcgacc ggcaccgtcg tctcgacgtt cgtgcgcggc gtgcgcgaac
     5461 cgaccaccac gatcgtctcg ttgagcagcg gaatcgtcgc gagctggaaa tcgagcttcg
     5521 acgtcgcacc gacctcgacc ttgatcgtct gctccagcga gctgaagccg ccaaaatcgg
     5581 cgcgtacctt cacgctgccc ggcttgacgt tctcgagctt gaagtgacct tcgtcgtcag
     5641 tggtcgcggt gatcccgctg ccgacgatcg tgacggtcgc accggctagc ggcgcatcga
     5701 gatccggcgc ctcgacggtg ccttcgatcg tgcccgttgt cgccggcggc ggctcgggcg
     5761 ccttctcggc gctctcctcg ggcgccggct tggtttcctc gtcggccgcc tcgccttctg
     5821 gctgcgcaga cgccgaacca gtccacaaca caacgaccac caagccgagt cgagtcaccc
     5881 gcacaggcgg ctatcgtaga ctgaaacggc gcgaccaggg tcagtcgacg cgcgcgaccg
     5941 tgccgggacc cgcgacctcg tcgatcgcgt gcagtagcag ctgcagcgaa tcggtcggcc
     6001 gcaggttcgc gcgcaggccg taaccgtcgg caacccagta cgccttgcgg gcgtatggat
     6061 agaacggctc gcgtgcgatc accgcgacgc gcttggcgcc ggctttacgc gcatcgacga
     6121 gcagcgcgcg catctgcgcg agcgtggtgt cggggtggaa gtgcagcgcg agcacctcgc
     6181 cattgccacg cagctcttcg agcacttgga tgcgttgttg gttcggtccg tagctgtgac
     6241 cgccaacgat tagatcgccg cgcgtcgaga actcggcttc cggcaggctc ggatcgtcat
     6301 cggcagcagc gcccgcgacc ggccagttgt ccggatcggc ctgccgctcg atgttcgaga
     6361 tcgtctccgc cgtctctgca tcgagcttct cccacgcgcg caccgtctgc ttccacgcgt
     6421 cgtcatcgcg gcacgcgaga cactcggtca ccgcgttgcg cgcgcgctcc atgccccggc
     6481 gcagtgcgag cgcttcgatc acgtcgcctt gcagctcggg caccacgctc ttgcactcgc
     6541 gtgcgagcgg acctccgcat aggcgcacga gatattgatc cggatcctcg cccggcttcg
     6601 ggacgatctc cggcgccttc gggtctttca cttcgagctg cttgtcggcc gtgatcgacg
     6661 gaccgagcaa cgacggccac accgtgagcg cgagcgcatc gatcttttcg cgcatcgact
     6721 tcgcctgcag ctgctggatt ggatgaatcg cgagcgtcac gagcttgagc tcggcttcgc
     6781 gcgcgaccgt gcgatccatc tgtggccgcg cggcctggag cgtctgcgcc atctcggcga
     6841 acgcatcggg tgtgcgcccc gccgcgacga acttgtcgac ggcaccgacg acgacggcgt
     6901 gcttgtagtc gtcggacggt ttcttattga cctcgatcgt gtcgcggctg cagccagcag
     6961 cgacgatcgc aacgacgagg acgagacggc gcatcatctc cagtacggcc tacgtagcaa
     7021 ggtggatgcc caccacactc cagcttttca cttcacgtac gccgtgccga gacaaatcgc
     7081 cagtttctcg ggatccagct gggtccctag cgccaccagt cgcccgttct cgccaacaat
     7141 gtcgtcgggt tgccggtcga gcggctcgct cgacacacgt aggcctaccc ggtgaatcgc
     7201 tgaccagctg ccgtcgccgg ttcgcacgat gcctttgatc cgcacgtagt tggcgggcaa
     7261 tgcggcgagc tgatcctcga gctcttcgag gtcgattcgg gcctcgacat tcagccaggt
     7321 actgtcgacg ccatgtgcat gactgtcggg atgccgacag ttttcgtcat gaacgtgagg
     7381 ctcgccggtg ggcacgtgct cgacatcggg atcggcgagt acccgttcta gccaggtcac
     7441 gtgctcctcg gtggttcctc gcaggatctg cgctgacggc gcgagcacgt gcgccgcttc
     7501 gatcgtcgct ttcgtgtcgg cgtcaccggc gagcgattcc ttcgtcacga gcagcacgtc
     7561 ggcgtaagcg acctgagcag cgaccgcgac actcacgccc cgcgacgctc ggaaattcgt
     7621 ggcgtcgaga agcgtgacca cgatcgcgag gcgcacgtaa tcacgcaccg gctcgcgggc
     7681 cagcgcccac gcgatcggca gcggctcggc aacgccggtc gtctcgagca cgatcgcttc
     7741 gagatcgggg ttcgccgtca cgagatcgat caccgtcttg tcgagctcgt cgccgagcac
     7801 gcagcacacg cagccgccgg gcagctcgat ctgacgcgcg gcttcggtgc cgagcaacgc
     7861 accgtcgatc ccgacttcac cgagctcgtt gacgatgacg ccgatcttgc cgtgcgcgtt
     7921 tcgcgctgcc cgatcagcca gcaggcgatt gagcagcgtc gttttcccgg cgcctagaaa
     7981 gccggtcagg atcacgagcg ggacgcgcac ggcagcagcc tagcgcgtaa tcactacgaa
     8041 gcgcgtgcct tgtactcgca cacgacgacg cggcgcttgc cggcaggcga cagcgggatg
     8101 ttgtcgacga tctcgatcgt gaacggcgcg cccggcaggt agcgctctgc gtgcgaccgg
     8161 agcagctgct cctcgcgcca cggcagcccg ccgccttgat agggcacgac cttgaacacg
     8221 acgcggccat cctcgtgctg gtgcacctgg aagctgcgcg cgacgtgacc gatcgtcgaa
     8281 aacagaatgt tgaacacgag gccgccgacg gcgcggccct gtccgtcgta catcgtctcg
     8341 gtgatgcggc cttggatctg accgatgcgt gacagaccgc gaccgcagcg gcagcgctcc
     8401 tcgacgcctg ccaccgcgag gtcaccattg acgtagcgga tcatcgggca cgcgaggttg
     8461 tgcagatcgg tgatcaccac ctcgccaacc tcgcccggac gcgccgggcg caccgtgttg
     8521 ccctcgcgga caacgacctc gacgatcagg ttctccatcg acgtgtgcag gccctcgtgc
     8581 gcctcgcact ccgacgccat cagcatcacc tcgcggcacc cgtaggtatc gaacgccgga
     8641 ccgaacgcgc gattgatcgc cgcgcgatcg tgcggcaaca aaccctcggc accggtgagc
     8701 accgggatat cgctccagtc gcgcaggttg cgcgcgttga tgaagtgcgc gagcgcgccg
     8761 gcaccactcg cgtatgcgac gatgcactgc ggcttgtagc ggcgcacgta gtcgacggcc
     8821 tcgagcaacg ccggttcgcc gcgcggcgtg cagtcgagaa acacctcgcg tttgacgcgg
     8881 cgatcgagct ccgtcttcgt gcgcttccac cacgtggtgc ccgcaggcgg cacgaagccc
     8941 cagtagtgaa acgagcgctt gccgatgccg tagccgcccc agccgtagcc gcgcaggcga
     9001 atcgcgtcgc gccaatgtcg cgactcggcg ttgtagagca cctcgacggg ctggcccgag
     9061 ctgccactcg tgaccttgcg gatcaccgcc gcgggtggcg cgtttgcggt gcgctccatc
     9121 atcgtcgcgc gcagcgtcgg tcgatcgagg agcggaagct tctggagatc caccggcgac
     9181 gcgaagtcct ccggacgcag gccgcgctcg tcgagcacgc gccggtaatg tgcggtgtgg
     9241 cgatacgcgt gacgaaggag gcgccgcaag aacccagttt gcagaccgtg cagctcgggg
     9301 agtgaccagt gcgatgtctc ctcgagatat cggagcagcg ggaccgtcgg acggccgcgc
     9361 gcagcttcga acgcggggaa caatgccttc tcgagcagct gtccatagag gtctctcatg
     9421 ctggtttctc gacgatcacg acctttcgtt tgcccgcttc cgtgagcggg atgtcctcga
     9481 cgtactcgat cgcaaagggt gcgtcgggca gatacttgtc ggcgaatgcg tgaatcgcgc
     9541 ggtggttctc gtcgggaagt cgacttccga tctgcggcac caccttcatc accacgctac
     9601 cgtcgagccg ctgcacgacc tggaacttct tcgcaacttg atccatcaca ccgaacaaga
     9661 tgttgaacac gaggccgcca accggattgc cgcggccatc gcgcagcgtc tcggtcacgc
     9721 ggccttcgat tggaccgatc cgcgtcaggc tgcgcccgca ggtgcactca cttggcgcgt
     9781 gcgcgaccgc gagatcgccg ttgagataac gaatcatcgg gcacgcgagg ttgtgcagat
     9841 ccgtgaccac cacctcgccg gtctcaccgg gacgcgccgg tcggtcgctg ccgtcgggca
     9901 agcgcacgac gatctcggtg accatcgtct ccatcgacgt gtgcataccg ttgtggtgct
     9961 cgcactcgga cgcgaccagc atggtctcgc ggcacccgta cgtctcgaat gcgggcccga
    10021 atgcctcgac gatcgcctcg cgatcgtgcg gccacagtcg ttcggcgccg acaagcactg
    10081 ggaagtctcg ccacgtgcgc agcttgttgt cgttcacgaa gcgcgcgagt gccgccgcgc
    10141 cagccgagta cgcgaccatc acctgcggct gatacgaccg cagcgtcttg accgcgccca
    10201 tcagcgcagc ttcgccgcgc ggcgtgcagt cgacgtagag gtcgcgcttg aacgcgcggt
    10261 cgagccagac cttgcggcga tgcatccagc tcttcgacgg tggcccgaag ccccagtaat
    10321 ggagcgcgcg catgccgatg cagtaaccgc cccaaccgta gccacgccag cgcgtcgcgt
    10381 cgcgccagtg ccgtgactcc tcgttgtact tcacgacgac gggctcgccg gtcgtgccgc
    10441 tcgtcgactt cttgatcgcg acacgcggcg gcgcaccaga cagtcgcgaa tccatcgtcg
    10501 cgcgcacggt gtcgcgatcg agcagcggca attccgcgag ctgctcgacg cgcacgacgt
    10561 cctcgggacg aatgccgcgc tcgtccatca cctcgcggta gtacgcggtg tgctggtacg
    10621 cgtgcctgag cagccggcgc aagaagccga cctgcagatc gtggagtgcg ggcagtgacc
    10681 aacgctccgt cgtctgcagg taacgcagca ggggaacagt tggcctgcca cgtgctgctt
    10741 cgaacgctgg aaacaacgcc ttcgctagca acggaccgta gagatccttc acaacgatat
    10801 ttttctaaca cccaccccac gtgctgtccc gccttcgcaa aaagaacctc caccagtggc
    10861 tgcccgatta cgcacggcac ctcgtgcgac gtgcgcgcac gccacagtcg aacgcaccgc
    10921 gccacattct gttcgcgctg tgtgaccact acgagccgct gcacggtcgt gctccggacg
    10981 acattggccg cacgcgtgtc gatgcgtggg tcgacggtta tccgaagcta cgtgagtttc
    11041 gcgatagcaa cggccggccg cctcgacacg gctggttctt tcccggcgaa gaatatcgtc
    11101 cgcactatct cgatggtctc gcgaagctcg cacgcgccgg ctttggcgaa gtcgagttcc
    11161 atcttcatca cgacggcgac acggcgcact cactcgcgcc gcgcatcaaa gaccatctcg
    11221 acacgttcgc gcagcacggt catctctcgc gccgcggtga cgcctatcag tgggcgttca
    11281 tccacggcaa ctggtcgctc gcgaacggac gtcctgatgg caaatggtgc ggcgtcgatg
    11341 acgagctcgt catgctgcac gagctcggtt gctacgtcga tctgacgttc ccctctgcac
    11401 ccgatccgtg tcagcccgac aaggtcaacc agatctactg gccgatcggc gacctgacga
    11461 agcggcggag ctacgcgcaa ggtgaacgcg cgcgggtcgg cgcgagctac gacgatcgct
    11521 tgctgatgat caccggcccg ctcgcgtttg cgcgcaaggg ccttggcgtg cggctcgaga
    11581 acggcgcact caccggtaac gatccgccca ccgcggcgcg cgtcgatacc tggatttcgc
    11641 aggggatcca catcgagggg cgcccggagt ggacgttcgt caaggttcac acccatggcg
    11701 cgatcgagaa gaccgcctcg tcgctactcg gcgagggtag ccgcgtgatg cacgccgcac
    11761 tgcagcgcta catgcgcgaa ggttggaagc tgcattacgt cacggcgcgg gagatgtata
    11821 acgtcgcgcg cgcggcgatg gatggcgtgt taggtgagcc cagcgacttc ttcgattaca
    11881 tcgtgaagcc gccccccatt gcgtcgtaaa ctattcgttg acgttgcccg ctaaacatct
    11941 atggattcgc ctcg
//
LOCUS       DASUFX010000279        11100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_598155,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000279 DASUFX010000000
VERSION     DASUFX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598155"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFV99_11085"
     CDS             complement(<1..362)
                     /locus_tag="VFV99_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5059897.1"
                     /translation="MSRAVVAAYVACALIWGTTWFAIRRCIGDGAYPTLAAVALRFVI
                     ASVVLLPIAVRARPWPRGSAWLYLVLAGVLDAAAYTLVYIGEERVSGGLAAVVYGTQP
                     LILALLLQVFRIENLSKR"
     gene            418..846
                     /locus_tag="VFV99_11090"
     CDS             418..846
                     /locus_tag="VFV99_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5059898.1"
                     /translation="MRTILLASVLALGAGAGCSKTDKAPPKTETASVANLPTITVDEV
                     DQKLASGDCVPVDANGTETRKKMGVVPGAVLLTDSETFQISELPADKSKQLIFYCANT
                     RCGASHEAAEKALTAGYTNVKVMPDGIAGWVKAGKKVQNI"
     gene            1002..1328
                     /locus_tag="VFV99_11095"
     CDS             1002..1328
                     /locus_tag="VFV99_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059899.1"
                     /translation="MALAACAVISTEPGDDDGSGSGSGSGSGSGSGSGSSKMAVCGQE
                     RDCTPDDNGCASYPYESLPSRCYDICYRGRCCEQYEGAWHLVIYDCARPNYDGGIGDA
                     EGIDAF"
     gene            complement(1325..2269)
                     /locus_tag="VFV99_11100"
     CDS             complement(1325..2269)
                     /locus_tag="VFV99_11100"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5059900.1"
                     /translation="MTSLPIAVVTGGAGFIGSHTVDRLMETGHRVVVLDNFSTGKLSN
                     LANWADSPALHVVECDVSDSIFAALDPITNQHGPVGRIVHLAAQVSVVQSIANPQRDA
                     EVNYGGTLQVLDYAKARGVKKIVFASSAAVYGDVTEMPVSEDAPKQPLSPYGINKYAS
                     ELALDYYANVHGVPATALRFFNVYGPRQDPSSPYSGVISIFTDRARAGRPLTIFGDGQ
                     QTRDFIYVGDVVRAITAALADGNARLVANVGMGCEISVLELARTIVDLCGGGSPIEHQ
                     PARAGEILKSRARVDRLRDALGIVAETKLVDGLRLTLG"
     gene            2302..3279
                     /locus_tag="VFV99_11105"
     CDS             2302..3279
                     /locus_tag="VFV99_11105"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5059901.1"
                     /translation="MRAIQIERIGGPEVMQLADVRIGEPGPGEVRVRHSAIGVNFIDT
                     YQRSGLYQVALPAVLGSEAAGVVEAVGPGVTHLAVGDRVGYAARTPGSYAEARVLDAT
                     PVFKLPDAIDFPRAAAMMLKGLTVQYLLRRTQPQGGLQAGDMIVWHAAAGGVGLIACQ
                     WAAALGLVVIATAGSDEKCALAKQHGATHTINYRTEDVNVRVREITGGRGVKVVYDSV
                     GKDTWERSLQCLAPFGLMVSFGNASGPVPPVNLLTLSPKSLHVTRPTLNTHTANRAVG
                     EQMAADLFAMVTSGKVKIEIGRSYKLADAAQAHRDLEARATTGSVVLIP"
     gene            complement(3269..3901)
                     /gene="udk"
                     /locus_tag="VFV99_11110"
     CDS             complement(3269..3901)
                     /gene="udk"
                     /locus_tag="VFV99_11110"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX5059902.1"
                     /translation="MGRSLVVGIAGGTGSGKTTVANKLAAAMPEGRCITIEHDAYYRD
                     QSHLPPDERATINYDHPASLESALLADHLRELRAGKAVDVPIYDFATHTRRADKRRVV
                     PAPVIIVEGILVFSDANLREQMDIKIFVDTDADIRLMRRIRRDLELRGRTFQSVRDQY
                     YATVRPMHLEYVEPTKRWADLIVPEGGDNRVALDVLLGQLGRIAFDGFKG"
     gene            complement(3915..5942)
                     /locus_tag="VFV99_11115"
     CDS             complement(3915..5942)
                     /locus_tag="VFV99_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059903.1"
                     /translation="MDPATQPTVLGEFRIQAILGEGGSGVVYDATWGPRRVALKVLHP
                     SLVGTGKERAQFLIEAQRLQQIMHPSVVKVLAVGQLPDGRPYLAMERLEGETLASVIA
                     RGALPPARALEMFGDLCSAVQALHDQGLVHRDLKPENVFVVADRHAVLLDFGIAKDLD
                     APASTTTQEGSVRGTPAYMAPERFFGQPAGIATDVYELALVLYTMLAGRLPWDNVADP
                     EARLQPRALCELVDVPEALDVEIRRALSTRAQNRPPSAVSLLEAVRTAASDTATEPEA
                     AETARLRSGAQATVSSTNAPGRAPGGRTEQRTPLAWAPTEAAKQTTQPPRRKRWPFVA
                     LGIAALAGGGIAVGVAMKSSPAKTVAHDAGAIAVVPDDAHHASKDPWAPKPNETPKAF
                     SLVEDKLTPQQYRAEAAAAVQKLANDTRLVFAVQIGEMRDVSATNDLLDTITSHPKVA
                     ALGLVLPPCVKGIAADAEWLVFGAPGLEQSPMGTLILRGRWRRRDVESCFADTVKTHV
                     SADGAKLYRVGDDGWLDFIDDHTAYVTLNTKLDAEGVHKLVKKGAGPVNKVKKTFAAL
                     PAARTISMVVDGATNDDWSMLSLPRGTDIYGWIRVEQTGLVLDLAADPHNDAAATAAQ
                     RRIQPQLDDLFENTNREAVGRIEVVRQQTAVHVRGNMTSLMLGIVTASISM"
     gene            6019..6639
                     /locus_tag="VFV99_11120"
     CDS             6019..6639
                     /locus_tag="VFV99_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5059904.1"
                     /translation="MIRLALAEDHTIVRWALREALSKADDIEVVGEAGTAAETLAMIQ
                     NVKPDVLLLDITLPDRSGFDVLAELRQLETAPLVVVLTWHTEPSYAARAIAAGAHGYV
                     NKAVEPNELLNSIRAVSRGEQVIPPGVEQLLAQGDGHPASALTAREQQVMEMLARGMT
                     NREIAEHLDISIKTVDTHRGHVLKKLGLRNNSELTRFAVKHGYVSL"
     gene            complement(6670..7746)
                     /locus_tag="VFV99_11125"
     CDS             complement(6670..7746)
                     /locus_tag="VFV99_11125"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5059905.1"
                     /translation="MKRLLLVALIGCGSPDTPPLRASPPAPPPAPRAVIVPVDAAPAV
                     TPPSDEVWLRGTTHVHAAPSGDSTEPVESVVRWYEQHGYDFIALTDHNRVSHAESTAK
                     LIVIPGIELTHNRNDCEPPGDESHRCRIHVNGLGVTAKPEGKLEWTDYHEKLRTTLYQ
                     RAFKTAHDLGASLIQINHPQYYWGMTPDVMIAIGKSAELVEVANQQFTKWNTGDANHP
                     STESLWDAALGSGLTLWGVATDDAHDYQADGGGKYPAGGGWVMVKARRDPLAILAALA
                     AGHFYASTGVTLSRAERVGDELVVDVAPGTQNQYTIAFIENGKTVATVNGPSAKHAVP
                     RTGYVRAVVTRDDGKQAWVQPVRP"
     gene            complement(7743..10022)
                     /locus_tag="VFV99_11130"
     CDS             complement(7743..10022)
                     /locus_tag="VFV99_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5059906.1"
                     /translation="MRWGSLLVLLAACTSKKHAPEAHDAGPHGKPTEAVTAARVADAA
                     AATPAKPARTEHTVWKLVDNRHTAHRSVDGGDLVLDATNVGFARYTRFGVPAVHWHLG
                     ATVGNERAALADAVAAIEVPIIPEQPAPTQITARVNAADAKQSLLVRVNGNKGRKQKL
                     AQIKLEAGWQTIAFPIEKAWIKPGENVIAFETTGKSKSKVGFSWIRVGAVKPPGDQNP
                     LAAATFDAKADTIELDEHASLTWYVAIPEGAHLVADVAKPCHIQVGARAGDQSFVGGL
                     LGSDEKRVDLSGIAGKTVRLSLTTRDCPRAKIVQPRITLHGPAPETLPKAEPPKYVIL
                     WVMDALRADKLPIFTPGARAQTPNFEELAKSSVVFRQYYVQGNESQTSHSSIWTGVYP
                     AVHNVRMAGVGGTWKIDKKLETLGEKLTAAGLFTTAVTGNGFVTTEGGYGRGFREYRN
                     MMREKGIINGIIFGEKIVDAALARLDKHRDQSTFLFLGTIDTHGPWIARKPWIDIYSP
                     GPYNGPFKEYGTAKDLGFMPGRMGCSIIPPKEDIERLRAIYDSAVSYHDQQVGRLVKQ
                     LKSWGIWDQTMLIITADHGEEFFEDSRCGHGGSLRDSLIRVPLLIHDPSRFPGGTIVE
                     EGAEGVDLLPTMLAALGQPAVANAQGDSLVPLAEGVGRGWPRPSYTSMYEYAHAMRIG
                     RWKMRVGHNGVPIVHDMVDDPDEQKDLTTVRPIARRMLTDNLGLFLALRKQWKKSAWG
                     VVSNISAAGATALDEAAAP"
     gene            complement(10088..10552)
                     /locus_tag="VFV99_11135"
     CDS             complement(10088..10552)
                     /locus_tag="VFV99_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059907.1"
                     /translation="MTAWMKLEASLRRLKRFRELSAEQQYVDDEIMLAKRQWAEVPLS
                     ASGMSLVWPPDILPLAQELGFEPDTRGSAPADPAMLAGIAAATRLRAKALSIQDAIES
                     AGGITNLRVLVAAKTITLTGSAANEVAAEHAVAIAMRIDGEDCTIINQLVVG"
     gene            complement(10921..>11100)
                     /locus_tag="VFV99_11140"
     CDS             complement(10921..>11100)
                     /locus_tag="VFV99_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059908.1"
                     /translation="GGGGGGRGPRRDDRGPRDDRGPRRDDRGPRRDGPPPEGWGVSGF
                     TNNPFAKLKGDDKKQ"
BASE COUNT         1820 a         3658 c         3695 g         1927 t
ORIGIN      
        1 tgtcgcttcg acaggttctc gatccgaaac acctggagca gcagcgcgag gatcagcggc
       61 tgcgtgccgt agacgacggc tgcgagccct cccgagacgc gctcctcgcc gatgtagacg
      121 agcgtgtacg cggcagcatc gagcacgccg gcaagcacga gatagagcca cgcggagccg
      181 cgcggccacg gccgagcacg caccgcgatc ggtaacagga cgacgctcgc aatcacgaag
      241 cgcaacgcga ccgcagcgag cgtcgggtac gcgccgtcgc cgatgcagcg acgaatcgca
      301 aaccacgtgg tgccccagat cagcgcgcag gcgacgtagg cggctacaac agctcgtgac
      361 acggactgtc gcagtgtcgc atcttgcggc gcttacgaca ttcggtatgt tcggttcatg
      421 cgaaccattc tgcttgcgtc cgtgttggcg ctcggcgccg gtgcgggctg ctccaagacc
      481 gacaaagcac cccccaagac cgagacggca tcggtggcga acctgccgac gatcacggtc
      541 gatgaagtcg atcagaagct ggcgagcggg gactgcgtgc cggtcgatgc gaacggaacc
      601 gagacgcgca agaagatggg cgtcgtaccc ggcgcagtgc tgctgaccga ctccgagacg
      661 ttccagatct ccgagctgcc ggcggacaag tcgaagcagc tgatcttcta ctgcgcgaac
      721 acgcgctgcg gcgcctcgca cgaagccgcc gagaaggcgc tgacggctgg ctacacgaac
      781 gtgaaggtca tgccggacgg catcgccgga tgggtcaagg ccggcaagaa ggtccagaac
      841 atctaagcgt tgcgcgaatc gtgcagcgca gcgaccgcga cagcgacgcc ctggtttcgc
      901 gagtcgcgcg cagctgccga gtgtgatctt cgcgaatttg cgtggcacgt agcggcaccc
      961 cggctgcaaa ctcggtcggc atgcgattgg cgtgtctctt ggtggctctc gctgcctgcg
     1021 cggtcatttc gaccgagccc ggcgatgacg acggcagcgg cagtgggagc ggcagcggca
     1081 gtgggagcgg cagcggtagc ggcagcagca agatggccgt ctgtggccag gaacgcgact
     1141 gcacgcccga cgataacggc tgcgcgtcgt atccctacga gagcctgcca tcgcgctgct
     1201 acgacatctg ttaccgcggc aggtgctgcg agcagtacga aggcgcgtgg cacctcgtca
     1261 tctacgactg cgcacgcccc aactacgacg gtggcatcgg cgacgccgag ggtatcgacg
     1321 cgttctagcc gagtgtcagc cgcagaccgt cgacgagctt tgtctcggcg acgatgccga
     1381 gcgcgtcgcg cagccgatcg acgcgagcac gcgacttgag gatctcaccc gcgcgggcag
     1441 gttggtgctc gatcggcgag ccgccgccgc acagatcgac gatcgtacgg gcgagctcga
     1501 gcaccgagat ttcgcagccc atgccgacgt tcgcgacgag ccgcgcgttg ccgtcggcga
     1561 gcgcagcggt gatcgcgcgc acgacgtcgc cgacatagat gaaatcgcgc gtctgttggc
     1621 cgtcgccgaa gatcgtgagc ggccgccctg cccgcgcgcg atcggtgaag atcgagatca
     1681 cgcccgagta cgggctcgac ggatcctgac gcgggccgta gacgttgaag aagcgcagcg
     1741 ctgtcgccgg cacgccgtgg acgttcgcgt agtaatcgag cgcgagctcg ctcgcgtact
     1801 tgttgatgcc gtacggcgac agcggttgct tcggtgcgtc ctcgctgacc ggcatctcgg
     1861 tgacatcgcc gtaaacggcg gccgacgacg cgaacacgat tttcttcacg ccgcgcgcct
     1921 tcgcatagtc gagcacctgc agcgtgccgc cgtagttgac ctccgcgtcg cgctgcggat
     1981 tggcgatcga ctgcacgacc gagacctgcg ccgccaggtg aacgatgcgc ccgacgggac
     2041 cgtgttgatt cgtgattgga tcgagcgccg cgaagatgct gtcagagaca tcgcattcga
     2101 cgacgtgcag cgcaggcgaa tcggcccagt tcgcgagatt ggatagcttg ccggtcgaga
     2161 agttgtcgag cacgacgacg cggtggccgg tctccatcaa gcggtcgacc gtgtgcgagc
     2221 caatgaatcc agcgccgccc gtaacaactg cgatcggtag agacgtcacc tccgcagttt
     2281 acctgctagc gttcgcgcgg catgcgagcg atccagatcg aacgaatcgg cggccccgaa
     2341 gtcatgcaac tggcggatgt gcgcatcggc gagcccgggc cgggcgaagt gcgcgtgcgc
     2401 cacagtgcga tcggcgtcaa cttcatcgat acgtaccaac gcagcggttt gtaccaagtt
     2461 gcgctcccgg cggtcctcgg ttcggaggca gcgggcgtcg tcgaggcagt gggcccgggc
     2521 gtcacgcacc tcgcggtcgg cgatcgcgtt ggctatgcgg cgcggacgcc gggcagctat
     2581 gccgaggcgc gcgtgctcga cgcgactcca gtgttcaagc tgcccgacgc gatcgacttt
     2641 ccgcgcgccg cggcgatgat gctgaagggc ctgaccgtgc agtacctgct gcggcggaca
     2701 caaccgcaag gcggcctgca ggcgggtgac atgatcgtgt ggcacgcggc cgcgggcggt
     2761 gtcggcctga tcgcgtgcca gtgggcggca gcgctcgggc tcgtcgtgat cgcgactgcc
     2821 ggctccgacg agaaatgcgc gctggcgaag cagcacggcg cgacccacac gatcaactac
     2881 cgaaccgagg acgtcaacgt gcgggtccgc gagatcaccg gcgggcgcgg cgtcaaagtg
     2941 gtctacgact ccgtcggcaa ggacacctgg gagcggtcgc tgcagtgcct cgcgccgttc
     3001 ggcctcatgg tgtcgttcgg caacgcctcc ggcccggtgc cgccggtgaa tctcctgacg
     3061 ctgtcaccga agtcgctgca cgtgacgcgg ccgacgctca acacgcacac ggcgaatcgc
     3121 gcggtcggcg agcagatggc ggcggacctc ttcgcgatgg tgacgagcgg caaggtgaag
     3181 atcgagatcg gcaggagcta caagctcgcc gacgcggcgc aggcgcatcg cgatctcgaa
     3241 gcgcgcgcga cgaccggctc ggtcgtgctc atcccttgaa gccgtcgaat gcgatccggc
     3301 cgagctggcc gagcagcacg tcgagcgcga cgcggttgtc gccgccctcg ggaacgatca
     3361 ggtccgccca gcgcttcgtc ggttcgacgt actcgagatg catcggccgc accgtcgcgt
     3421 agtactgatc gcgcaccgac tggaacgtac gcccgcgcag ctcgaggtcg cggcggatcc
     3481 ggcgcatcag ccggatgtcg gcgtcggtgt cgacgaagat cttgatgtcc atctgctcgc
     3541 gcaggttcgc atcggagaac accaggatgc cctcgacgat gatcaccggt gccggtacga
     3601 cgcggcgctt gtcggcgcgc cgggtatgcg tcgcgaagtc gtagatcggg acatcgaccg
     3661 ctttgccggc gcggagctcg cgcaggtggt cggccagcag cgcgctctcg agcgacgccg
     3721 gatggtcgta gttgatcgtc gcgcgctcgt cgggcggtaa atgactctgg tcgcggtagt
     3781 acgcgtcgtg ctcgatcgtg atgcagcgcc cctcgggcat cgccgctgcg agcttgttgg
     3841 ccaccgtggt cttgccggag ccggtaccac cggcgatccc gacgacgagc gaacgtccca
     3901 ttgcccgagc tatctcacat actgatgctg gctgtcacga tgccaagcat caacgacgtc
     3961 atgttgccgc gcacgtggac cgcggtttgc tgccgcacga cctcgatgcg gccgaccgct
     4021 tcccgattcg tgttctcgaa caaatcgtcg agctgcggct ggatccggcg ctgtgcggcg
     4081 gtagcggccg catcgttgtg cgggtccgcg gcgagatcga gcacgagccc cgtctgctcg
     4141 acgcgaatcc agccgtaaat gtcggtcccg cgcggcagcg acagcatcga ccaatcgtcg
     4201 ttcgtggcac cgtcgacgac catcgagatc gtgcgcgccg cgggcagcgc cgcgaacgtc
     4261 ttcttcacct tgttgaccgg gccggcaccc ttcttgacga gcttgtgcac gccctcggcg
     4321 tcgagcttcg tgttgagcgt gacgtacgcg gtgtgatcgt cgatgaagtc gagccagccg
     4381 tcgtcgccga ctcggtagag cttcgcgccg tcggcactga cgtgcgtctt cacggtgtcg
     4441 gcaaagcaac tctcgacatc gcgccgccgc cagcgcccgc gcaggatcag cgtgcccatc
     4501 ggtgactgct cgaggccagg cgcgccgaac acgagccact cggcgtccgc cgcgatgccc
     4561 ttcacgcacg gcggcaacac gaggccgagc gcggcgacct tgggatggct ggtgatcgtg
     4621 tcgagcagat cattggtcgc gctgacgtcg cgcatctcgc cgatctgcac cgcgaacacg
     4681 aggcgcgtgt cgttcgcgag tttctgcacg gcggccgcgg cctcggcgcg atactgctgc
     4741 ggcgtgagct tgtcctcgac gagcgagaat gctttcggcg tctcgttcgg cttcggcgcc
     4801 cacggatcct tgctcgcgtg atgcgcatcg tcaggaacga ccgcgatggc gccggcatcg
     4861 tgcgccacgg ttttcgccgg agacgatttc atcgcaaccc ccaccgcgat ccccccgccc
     4921 gcgagcgcag cgatcccgag cgcgacgaat ggccagcgct tgcgccgcgg cggctgcgtc
     4981 gtttgcttcg ccgcttccgt cggtgcccac gcgagcggcg ttcgctgctc ggtgcgaccg
     5041 cccggtgcgc gcccgggcgc gttcgtcgac gagacagtgg cctgtgctcc cgagcgaagt
     5101 cgcgcagtct cggccgcttc cggctccgtg gcggtatcgc tcgcagctgt gcgcacggct
     5161 tcaagcagcg atacggcgct cggcgggcga ttctgcgcac gcgtcgacag tgctcgccga
     5221 atttcgacat cgagcgcttc tggcacgtcc acgagctcgc aaagtgcacg cggctgcaga
     5281 cgcgcttcgg gatcggcaac gttgtcccat ggcaaccgtc ccgccagcat cgtgtagagc
     5341 acgagagcga gctcgtagac gtcggtcgca attcctgcgg gctgaccgaa aaagcgttct
     5401 ggagccatat aggccggcgt gccgcgcacg ctgccttctt gcgtggtggt cgatgcgggc
     5461 gcatcgagat ccttcgcgat gccgaagtcg agcagcacag cgtgacgatc cgccaccaca
     5521 aaaacgtttt ccggtttgag atcgcgatgc acgaggccct ggtcgtgaag agcctgcacc
     5581 gcagaacaca gatcgccgaa catctcgagc gcgcgtgcag gcgggagggc accgcgcgcg
     5641 atgacgctcg cgagcgtctc gccctcgagg cgctccatgg cgaggtaggg gcgcccatct
     5701 ggcagctgcc cgaccgcgag gaccttcacc accgacgggt gcatgatctg ttgcagtcgc
     5761 tgggcctcga tcaggaactg cgcgcgttct ttaccggtgc cgacgagact cggatgcagc
     5821 accttgagcg cgacgcgacg tggtccccaa gttgcgtcgt atacaacgcc gcttccgccc
     5881 tctccgagaa tcgcctggat tcggaactcg ccaagaacgg tgggttgcgt cgctgggtcc
     5941 acggaagggc cccctcacca taacgcagct ggtctcggcc cgggggttgc tactttcctg
     6001 catgcagcgg aggacacaat gatccgactc gcattagctg aagaccacac catcgttcgt
     6061 tgggccttgc gagaagccct cagcaaggcc gacgacatcg aagttgtcgg agaagccggc
     6121 accgcggcgg agacgctcgc gatgatccaa aacgtcaaac ctgacgtcct gcttctcgac
     6181 atcaccctgc ccgaccgctc gggctttgac gtactcgccg agttgcgcca gttagagact
     6241 gcgccactcg tcgtggtgct gacctggcac accgagccca gctacgccgc acgtgcgatc
     6301 gcagctggcg cccacggtta cgtcaacaaa gcggtcgagc caaacgagct gctcaattcg
     6361 atccgtgcag tctcgcgtgg cgagcaagtc atcccgccag gcgtcgagca attgctcgcg
     6421 caaggcgatg gccatcctgc aagtgcgctg acggcgcgcg agcagcaggt catggagatg
     6481 ctcgcacgcg gcatgacaaa ccgcgagatc gccgagcatc tcgatatcag catcaagacc
     6541 gtcgataccc atcgcggtca cgtgctgaag aagctgggcc tacgtaacaa ctccgagctc
     6601 actcgattcg cagtcaagca cggttacgtg tcgctctagt cggatggatg gctctccgcc
     6661 gtcctccgac tacggtcgca cgggctgtac ccaggcttgc tttccgtcgt cacgagtgac
     6721 gacggcgcgg acgtaacccg ttcgcggcac ggcatgcttg gcgctcggtc cgttcaccgt
     6781 agcgaccgtc tttccgttct cgatgaacgc gatcgtgtac tggttctgcg tgcctggcgc
     6841 gacgtcgacg acgagctcgt cgccgacgcg ttcagcacgt gacagcgtca caccggtcga
     6901 cgcgtagaag tgaccagccg cgagcgcagc gagaatggcg agcgggtcgc ggcgtgcctt
     6961 caccatcacc cagcctccgc ccgcggggta tttccctccg ccatcggctt ggtagtcgtg
     7021 cgcatcgtcg gtcgctacgc cccacagcgt gagcccgctg ccgagcgctg cgtcccacag
     7081 cgactccgtc gacggatgat tcgcatcgcc ggtattccat ttcgtgaatt gctggtttgc
     7141 gacctcgacg agctcggcgc tcttgccgat cgcgatcatc acgtcggggg tcatgcccca
     7201 gtagtactgg ggatgattga tctggatgag gctcgcgccg aggtcgtgcg ccgtcttgaa
     7261 cgcgcgctgg tagagcgtgg tgcgcagctt ctcgtggtag tcggtccact cgagcttgcc
     7321 ctcgggcttc gccgtgacgc caagcccgtt gacgtgaatg cggcagcggt gggattcgtc
     7381 gcccggcggt tcgcaatcgt tgcgattgtg ggtgagctcg atgcccggaa tcacgatcag
     7441 cttcgcggtg ctctccgcat ggctgactcg attgtgatcg gtgagcgcga tgaagtcgta
     7501 gccatgttgt tcataccaac ggacaacgct ctcgacgggc tcggtgctgt cgcccgacgg
     7561 cgcagcgtgg acgtgcgtcg tgccgcgcag ccagacttcg tcggacggcg gcgtcaccgc
     7621 aggggcggcg tcgacaggaa cgattaccgc acgtggcgcc ggcggcggcg caggtgggct
     7681 cgcgcggagc ggcggcgtat cgggcgagcc gcaaccgatg agcgcgacga gcaacaggcg
     7741 cttcacggcg ctgcagcctc gtccagcgcg gttgcaccag ccgccgagat gttcgagacc
     7801 acgccccacg ccgacttctt ccattgcttg cgcagtgcga ggaacaaccc gaggttgtcg
     7861 gtgagcatgc ggcgcgcgat cggccgcacg gtggtcagat ccttctgctc gtcggggtcg
     7921 tcgaccatgt cgtgcacgat cggcacgccg ttgtgaccga cgcgcatctt ccagcggcca
     7981 attcgcatcg cgtgcgcgta ctcgtacatc gacgtgtacg acgggcgcgg ccaaccccga
     8041 ccgacgcctt cggcgagcgg cacgagcgaa tcaccctgcg cgttcgcgac ggccggctgg
     8101 ccgagcgcgg cgagcatcgt tggcaagagg tcgacacctt cggcaccttc ctcgacgatg
     8161 gtgccgccgg ggaaccgcga tggatcgtgg atgagcagcg gtacgcggat cagcgagtcg
     8221 cgcaacgagc cgccgtggcc gcaccgcgag tcctcgaaga actcctcgcc atgatcggct
     8281 gtgatgatca gcatcgtctg atcccagatg ccccacgact tgagttgctt gacgaggcga
     8341 ccgacctgct gatcgtggta gctgacagcg ctgtcgtaga tcgcgcgcag tcgctcgata
     8401 tcttccttcg gcgggatgat gctgcagccc atcctgcccg gcatgaaccc gaggtccttc
     8461 gcggtgccgt actccttgaa cgggccgttg tacggacccg gcgagtagat gtcgatccac
     8521 ggtttacgcg cgatccacgg cccgtgggtg tcgatcgtgc cgaggaacaa gaacgtcgat
     8581 tgatcgcgat gcttgtcgag gcgagcgagt gcagcgtcga cgatcttctc gccgaagatg
     8641 atcccgttga tgatgccctt ctcgcgcatc atgttgcggt actcgcggaa gccgcggcca
     8701 tacccgccct cggtcgtgac gaagccgttg ccggtgacgg cagtcgtgaa caggcccgcg
     8761 gcagtgagct tctcgccgag cgtctcgagc ttcttgtcga tcttccacgt gccgccgaca
     8821 ccggccatcc ggacgttgtg gacggctgga tagacaccgg tccagatgct cgagtgactg
     8881 gtctgcgatt cgttgccttg cacgtagtac tggcggaaca ccacgctcga tttcgcgagc
     8941 tcctcgaagt tcggcgtctg cgcgcgtgca cccggcgtga agatcggaag cttgtcggcg
     9001 cgtagcgcgt ccatgaccca caggatcacg tacttcggcg gctcggcttt cggcagcgtc
     9061 tctggcgcag ggccgtgcag cgtgatgcgt ggctgcacga tcttcgcgcg cgggcagtcg
     9121 cgcgtcgtca acgagagccg cacggtcttg ccggcaatgc ccgacaggtc gacgcgtttc
     9181 tcgtcggagc cgagcaagcc accgacgaaa ctctgatcgc cggcgcgtgc accgacttgg
     9241 atatggcagg gcttcgcgac atcagcgacg agatgcgcgc cctcgggtat cgcgacgtac
     9301 caggtcaggc tcgcgtgctc gtcgagctcg atcgtgtcgg ctttcgcgtc gaacgtcgcc
     9361 gcggcgagcg gattctgatc gcccggcggc ttcaccgcgc cgacgcgaat ccacgagaag
     9421 ccgaccttcg acttgctctt gcccgtggtc tcgaacgcga tcacgttctc gcccggtttg
     9481 atccacgcct tctcgatcgg aaacgcgatc gtctgccagc ccgcttcgag cttgatctgc
     9541 gcgagcttct gcttgcggcc cttgttgccg ttgacgcgca cgagcagcga ctgcttggcg
     9601 tcggcagcgt tgacgcgcgc ggtgatctgc gtcggcgcgg gctgctcggg aatgatcggt
     9661 acttcgatcg ccgccaccgc atcggcgagc gcagcgcgtt cgttgcccac cgtcgcgccg
     9721 agatgccagt gcaccgccgg tacgccaaac cgcgtgtagc gcgcgaagcc gacgttcgtg
     9781 gcgtcgagca caaggtcacc gccatcgacg ctgcgatgtg cggtatgccg gttgtcgacg
     9841 agcttccaca ccgtgtgctc ggtgcgggca ggcttcgcgg gcgtcgcggc ggccgcatcc
     9901 gcaaccctgg cggcagtaac cgcttcggtt ggcttgccgt gcggccctgc gtcgtgggcc
     9961 tcgggcgcat gcttcttgct cgtgcatgcc gcgagcagca cgaggaggga tccccaccgc
    10021 atcgttaggt tatagcgtga tgcagagcgc gctgctcgcc gttcgagagt cgttggcgat
    10081 cttacggtta cccgacaacg agctggttga tgatcgtgca gtcttcgccg tcgatgcgca
    10141 tcgcaatcgc gaccgcatgc tcggctgcca cctcgtttgc cgccgacccg gtcaacgtga
    10201 tcgtcttcgc agccacgagc actcgcaggt tcgtgatccc gccggcggac tcgatcgcgt
    10261 cctggatcga gagcgccttc gcgcgtaggc gcgtcgccgc cgcgatgccc gccaacatcg
    10321 cgggatctgc aggggcacta ccgcgcgtgt cgggctcgaa gccgagctcc tgggcgagcg
    10381 gcagaatgtc cggcggccag acgagtgaca tgcccgacgc cgagagcggc acctcagccc
    10441 attgccgctt cgcgagcatg atctcgtcgt cgacgtactg ctgctcggcg ctcagctcgc
    10501 ggaatcgctt caggcgccgc aagctcgctt cgagcttcat ccacgcagtc acgcggcgag
    10561 aatagcgcgg ccgtcgtctg tgtcgcgtcg cccggggcac cgaaccgaag cgacggagat
    10621 tctcgcgcac gccctagaat tgaaccgcca agacgccgag gccgccaaga tcggagatcg
    10681 atcttgatcg atcttgatta cgccctgcac gcacgatcgc cacatcgaac gcgggccggt
    10741 gcgcgtgctc gactcgggcg cgtgctcgac tcgggcgcgc gctcgactcg gcgcgtgttc
    10801 gacgcgggca cctgcgagcg gtccgtgccg aaacctcgtt cttctgggct tggcgtccgc
    10861 gtcgccttgg cggttcaact caccggcgcg ttggtggcgg cgatttggcg gttcagcccg
    10921 ctactgcttc ttgtcgtcgc ccttgagctt cgcgaacgga ttgttcgtga agcccgagac
    10981 gccccagccc tccggtggcg ggccgtcacg gcgcggaccc cgatcgtcgc ggcgcgggcc
    11041 acggtcgtcg cgcgggccac gatcatcgcg gcgcggacca cgaccaccac cgccgccgcc
//
LOCUS       DASUFX010000280         8303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1849227, whole genome shotgun sequence.
ACCESSION   DASUFX010000280 DASUFX010000000
VERSION     DASUFX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849227"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFV99_11145"
     CDS             complement(<1..758)
                     /locus_tag="VFV99_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059909.1"
                     /translation="MYALPYILLVRLHRLALDARRRQRGLPYYGYSFAAALLASLLIS
                     AGLFAAWAMFYVGMWYLGIAVIVIFVTPPLQPILSRRVLVPLGLVRTSFWVAHFVSVD
                     DSDAYGLCCATWAHSMKPKPADEAWLIAKRDKRVPLGDAEIIMTALLAAARGDAETTR
                     QLMRSTAEMVENHPLVRELAGEWLAVDAADRGAWREIYDDSITARWPASALTFFLEGC
                     AARKLEAAGAPSAFEVRVRWLLAPYRRTTRQFLA"
     gene            complement(759..1088)
                     /locus_tag="VFV99_11150"
     CDS             complement(759..1088)
                     /locus_tag="VFV99_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059910.1"
                     /translation="MSAKIAENPFFVLGISPDASRIEIEREAQKLLGMLELNFPDALT
                     YATPLGPRPRTPELVRAAVAALRDPYQRLVAELWARHAPAHADEPASIEEEPKRPSLR
                     PALGWRP"
     gene            complement(1085..3526)
                     /locus_tag="VFV99_11155"
     CDS             complement(1085..3526)
                     /locus_tag="VFV99_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5059911.1"
                     /translation="MGETPLFVGIDLGTTNSAAAVFDGEHVAVVRNAQGASVTPSVVR
                     IDKQGRVTVGTRARRFVEQDPANTAAEFKRLMGTDQQIDFPAAGVKKKPQELSAEVLK
                     ALRQDIADQLGATIERAVISVPALFELPQSSATSEAARLAGFERVELLQEPIASALAA
                     GWRAEDDGGGSWLVFDFGGGTFDASLLETRDGLLRVVGHDGDNFLGGRDFDWRITEHL
                     ASQLTVVPSRKNPEHSAALRALRIAAEDAKIELSRSDRAQVTLAQPLVIDGQDVEVDL
                     ELTRATVESLCAPLVDRALDVCVRLLTAQGLGPGRLAKVVLVGGPTVMPIVRQRVAAR
                     LEAPIAEGHDPMTLVAQGAALYAATAGLDGRGVAQTHTAGRQVWLQYPAVSSDLTPHV
                     VGKFVGDNPPAKVKLVRNDGAWSSADATVGEDGTWLTSVTLLPRRACTFTIEAVNAAG
                     DKIAVSPPSLTIVQGLTIGDPPLSRTLGVALANGHVQTYLERGAPLPARRTFTHHTVE
                     TVAKGSKESVLRIPIVQGELDQAHLCRLVGTLDIGGDTVKDTVPTGSPVEVTIELDRG
                     GRLSARALVPVIGQVFEQVAHLLVPDASPEALDSAIRDLRRQLMDLRTDAFRHGLGQV
                     IEKLDRLEARLSEAERDIDAAHGGDADAAQRARRALLDVDATMAEADLARKWPELDAA
                     ARQAAITASSTVGFHGTDAERALLQDVLAAMDKARKDKDAPELERQMRLAQRLSSAAF
                     NRTPEAWEWYFEDAASEVSRARDLPKANRLVAEGKVAIDRGDTEELRRVVKALWQLLP
                     EDSEARKRGFDSGVR"
     gene            complement(3623..3841)
                     /gene="infA"
                     /locus_tag="VFV99_11160"
     CDS             complement(3623..3841)
                     /gene="infA"
                     /locus_tag="VFV99_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5059912.1"
                     /translation="MSKEELVHLDGEVILIAKGGNFRVKLDNGHEVLAKLAGKVRRHR
                     IRVVLGDRVTVAVSPYDPTRGFIVYRQR"
     gene            3921..5594
                     /locus_tag="VFV99_11165"
     CDS             3921..5594
                     /locus_tag="VFV99_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059913.1"
                     /translation="MDRQPPRATSDEIDLYIRTYYSLLRSSGDVRVRAFEEAHLFSRS
                     SLHLGAEDPNPDLAAFAYSAARLPDCMPNIRRIVLGQSPVQFRAAGFPIERWQVVRTR
                     GRRRPLRWDGGQTLAVFVASGSDIDDLVPILTAYQIEWNKLRERLIAARVTDVADDAA
                     IAAALGTDDVDVARLRGALGPSSDQILRDMIAHDCDVFVRLLDGSFREYQRSAQRWWN
                     AILPSVPQGRPVYFVSSNSHSLANLLGGYARGHRDEIVAFLRRRNFENLAPAYDEAIA
                     AGKEADATAILYYSLRAFIHEPGNNGRMDLVQKLDLEAGIRAIGSPGKIDVDAQVIDL
                     AELKVENLDPRVCVPGIEKLVNSRAVIINIDYPLGMASYHHLSAIALGVDDLRGIYIM
                     GKAATLNGRVGDVMISKVVHDEHSRNTYLFKNALVAADVQPFMRHGTVLDNQKALTVR
                     SAFLQNREYMDVFYREGYTVMEMEAGPYLSGAYEIAEPRRHPQNEIVSLVDQSNFELG
                     VIHYASDTPYSRRQSLLSKSLSYFGMESTYGCAIAIARRIFANELARQT"
     gene            5591..6856
                     /locus_tag="VFV99_11170"
     CDS             5591..6856
                     /locus_tag="VFV99_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5059914.1"
                     /translation="MTDKADVLVIGAGVSGLGFANWWRELHPDARVVVCESEADPGGY
                     CRTIVRDGFVWDYSGHFFHFKDPSVEQWLRAKMPGEDIRTVERVAKIRYAGRDIDFPF
                     QTHIHQLPIDEFLECLTALFFRPTSDEPPHSFGEMLYRRLGAGITDKFLRPYNEKLYA
                     TNLDTLDVDAMGRFFPHADIADIIGNMRPGHRKHGYNATFTYPAGGAVRYIEALMRAL
                     PAGTVSCDEPITSIDLDSRIATTPKRTIAFDRIVSSAPLPALAYMCKLEHDPAVFTSN
                     QVLVFNLGFDRKGMRDIHWMYFPDPATVFYRVGWYDNILDGDRMSLYVEIGAADGATF
                     EVDALRQRVLTDLAREGIVTDHKLVAEHHVALDPAYVHITRASLAETKRLRDLLASKG
                     VHSVGRYGGWTYCSIEDNLIETRALARSL"
     gene            complement(6857..>8303)
                     /locus_tag="VFV99_11175"
     CDS             complement(6857..>8303)
                     /locus_tag="VFV99_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059915.1"
                     /translation="KSPGSAKTSEAHAIREMAPDPWDRGKCTPIVSKLSRGNGDDTGV
                     DAVEAAWNDLDKAAQKTALAFVQHVDGSAGAEDPLPGALDALDAARAKLRSVAEMPAN
                     QQTGAVLPSAQMVPLVDGKDPVTSVEVTSPPSAHGVVLYGKTGSREIQISLSPGGNPS
                     VTRVGPGSMRSVPDATWGATAGDLTLKVTGSKRDSVGELLAGAMDAEGVIATPTKLAV
                     TSPIPPTGNMVSDNPTLKSGERIGSVTVAAVSGTLNEGTIIYGAFQSLVVAHAKANVL
                     TPATTLKIDNALASSDLDGRVALVWTTPDKVSHATLFKTGGEESFELPATFGAQPCLT
                     TDRVWLPGNEHEVIAFGGGRPLDRVQLPSFYELQGCTADTAIVRNPEAHRDVAICTDK
                     CRTVTIPSGAPEYANVTAIKGKLVAIASHDGVVGVWAEDKPPVFYTLPARAMPVYDRR
                     SAPAMALTDGKVIDVIALSDRKYVVVRIPAP"
BASE COUNT         1327 a         2846 c         2734 g         1396 t
ORIGIN      
        1 atcgcgagga actgacgcgt ggtgcggcga tacggcgcga gtagccaacg cacgcggacc
       61 tcgaacgcgc tcggcgcgcc tgccgcttcg agcttgcgcg cggcacagcc ctcgaggaag
      121 aacgtcagcg cgctcgccgg ccagcgtgcg gtgatcgagt cgtcgtagat ctcgcgccac
      181 gcaccgcggt cggcggcgtc gacggcgagc cactcgccgg cgagctcgcg aacgagtgga
      241 tggttctcga ccatctcggc ggtcgagcgc atcagctggc gcgtcgtctc ggcgtcaccg
      301 cgtgcggccg cgagtaacgc ggtcatgatg atctcggcat cgccgagcgg tacgcgcttg
      361 tcgcgcttcg cgatcagcca ggcctcgtcg gcgggcttcg gcttcatcga atgcgcccac
      421 gtcgcgcagc acagcccgta cgcatcgctg tcgtccacgc tgacgaagtg cgcgacccag
      481 aacgacgtgc gcacgagccc gagtgggacg agcacgcgcc gcgacaggat cggctgcagt
      541 ggcggcgtca cgaagatcac gatcaccgcg atgccgaggt accacatgcc gacgtaaaac
      601 atcgcccacg cggcgaatag cccggcgctg atcaacagcg aggcgagcag cgctgctgcg
      661 aacgaatacc cgtagtacgg caaaccgcgc tggcgccggc gcgcatcgag cgcgagccga
      721 tgcaggcgaa cgagcaagat atacggcagc gcgtacaact atggtctcca gcccagcgcg
      781 ggccgtagcg acggtcgctt cggttcctct tcgatgctcg ccggttcatc ggcgtgagcg
      841 ggcgcgtgtc gcgcccacag ctcggcgacg aggcgttgat acggatcgcg gagtgccgcc
      901 acggccgcac gcaccagctc cggtgtgcgc gggcgcggcc cgagcggcgt cgcgtacgtc
      961 agcgcgtccg ggaaattcag ctcgagcatg ccgagcagct tctgggcctc gcgctcgatc
     1021 tcgatccggc tcgcgtcggg cgagatgccg aggacgaaga acggattctc ggcgatcttc
     1081 gcgctcaccg gactcccgaa tcgaagccac gcttgcgcgc ttcgctgtct tccggcaaca
     1141 gctgccacag cgccttcacg acgcggcgca gctcctcggt gtctccgcga tcgatcgcga
     1201 ctttgccttc ggcgacgagc cggttcgcct tcggcaggtc gcgcgcacgc gacacctccg
     1261 acgcggcgtc ctcgaagtac cactcccagg cttccggcgt gcgattgaac gccgcgctcg
     1321 agagacgctg cgcgaggcgc atctggcgct cgagctccgg tgcgtccttg tccttgcgcg
     1381 ccttgtccat cgcagccaac acgtcctgca agagcgcgcg ctcggcgtcg gtgccatgga
     1441 aaccgaccgt cgacgatgcg gtgatcgccg cttgccgcgc ggccgcgtcg agctcgggcc
     1501 acttgcgcgc gagatccgcc tcggccatcg tcgcgtcgac gtcgagcagt gcgcggcgtg
     1561 cgcgttgcgc tgcgtcggca tcgccgccgt gtgccgcgtc gatgtcgcgc tccgcttcgg
     1621 acaaccgcgc ttcgaggcga tcgagctttt cgatcacctg gccgaggcca tgccggaatg
     1681 cgtcggtgcg cagatccatc agctgccggc gcaggtcgcg gatcgcgctg tcgagcgcct
     1741 cgggtgacgc gtccggcacg agcagatgcg cgacctgctc gaacacctga ccgatcaccg
     1801 gcacgagtgc gcgcgcactc aggcgaccgc cgcggtcgag ctcgatcgtc acttcgaccg
     1861 gcgaaccggt cggcaccgtg tccttcacgg tatcgccgcc gatgtcgagc gtgccgacga
     1921 gccggcagag gtgtgcctgg tcgagctcgc cttgcacgat cggaatccgc agcacgcttt
     1981 ctttgctgcc cttcgcgacc gtctcgacgg tgtgatgcgt gaacgtacgg cgcgcgggca
     2041 ggggagcacc gcgctcgagg tacgtctgaa cgtggccgtt cgcgagcgcg acgccgagcg
     2101 tgcgcgacag tggcgggtca ccgatcgtca ggccctgcac gatcgtgagc gacggcgggg
     2161 acacggcgat cttgtcgcct gcggcattca ccgcttcgat cgtgaacgtg cacgcgcggc
     2221 gcggcagcaa tgtcacgctc gtgagccacg tgccgtcttc gccgaccgtc gcatcggcgc
     2281 tcgaccatgc tccgtcgttg cgcacgagct tcaccttggc cggcgggttg tcgccgacga
     2341 acttgccgac aacgtgcggc gtgagatccg acgacaccgc cggatactgc agccacacct
     2401 ggcggcccgc cgtatgcgtc tgcgccacgc cacggccgtc gaggcctgcg gttgccgcgt
     2461 agagcgcggc gccttgcgcg acgagcgtca tcggatcgtg gccttcggca atcggcgctt
     2521 cgagacgcgc cgcgacgcgc tggcgcacga tcggcatcac cgtcggtccc ccgacgagca
     2581 cgaccttcgc gagccggccg gggccaaggc cctgcgcggt gagcaggcgc acgcagacat
     2641 cgagcgcgcg atcgacgagc ggcgcgcaca ggctctcgac cgtcgcgcgc gtcagctcga
     2701 gatcgacctc gacgtcctga ccgtcgatca cgagcggctg ggcgagcgtg acctgtgcac
     2761 ggtcgctgcg cgacagctcg atcttcgcgt cctcggctgc gatccgtagc gcgcgcaatg
     2821 ccgccgagtg ctcggggttc ttgcggctcg gcacgaccgt cagctgcgac gcgaggtgct
     2881 cggtgatccg ccagtcgaag tcgcggccgc cgaggaagtt gtcgccatcg tgaccgacga
     2941 cgcgcagcag accatcgcga gtctccagca acgacgcgtc gaacgtgccg ccgccgaaat
     3001 cgaacacgag ccacgagcca ccgccatcgt cctcggcgcg ccagcccgct gcgagcgccg
     3061 atgcgatcgg ctcctggagc aattcgacgc gctcgaaccc ggcgagtcgg gcagcctccg
     3121 acgtcgcaga gctctgcggc agctcgaaca gcgcgggcac tgaaatcacc gcgcgctcga
     3181 tggtggcgcc gagttgatcc gcgatgtcct gccgcagtgc cttgaggact tcggccgata
     3241 gctcttgcgg cttcttcttc acgcccgccg cgggaaaatc gatctgctga tcggtgccca
     3301 tcagccgctt gaactccgcg gccgtgttcg ccggatcttg ttcgacgaaa cggcgcgcgc
     3361 gggtgccgac cgtgacgcgt ccttgtttgt cgatgcgcac gaccgagggc gtgacgctcg
     3421 cgccttgcgc gttgcgtacg accgccacgt gctcgccatc gaacacagca gctgccgagt
     3481 tcgtcgtccc cagatcgatc cctacaaaca gcggagtttc acccaccgcc ggagtctagc
     3541 gcaggggtca ggctcgagtt gtacgagttg ccagactgac aactcatacg agtcatgtct
     3601 gacccctatc gcgaagctgt gcttagcgct ggcggtagac gatgaagccg cgggtcgggt
     3661 cgtacggcga cacggcgacg gtcacgcggt caccgagcac cacgcggatg cggtgccggc
     3721 gcactttgcc cgcgagcttg gcgaggacct cgtggccgtt gtcgagcttg acgcgaaagt
     3781 tgccgccctt cgcgatcaag atgacctcgc catcgagatg aactagctct tccttactca
     3841 caggtgggcc cttggttagc actaacgacc ggccaggaaa agcgctttgg gccccaaggt
     3901 gatctaccct ttgccggtaa atggaccgcc agcctcctcg cgccacaagc gacgagatcg
     3961 acctgtatat ccggacctac tactcgcttc tgaggtccag cggcgacgtg cgcgttcgcg
     4021 cgttcgagga ggctcacctg ttctcacggt cgagcctgca cctcggtgcc gaggacccca
     4081 atcccgacct tgcggcgttt gcgtacagcg ctgcgcgcct tcctgactgc atgccgaaca
     4141 tccggcgcat cgtgctcggc cagagcccgg tgcagttccg ggcagcagga ttcccgatcg
     4201 aacgctggca ggtcgtccgc acgcgcggcc ggcgccggcc actgcgctgg gacggtggcc
     4261 agacgctggc ggtgttcgtc gcatcgggct cggatatcga cgacctggtt ccgatcctca
     4321 ccgcgtatca gatcgagtgg aacaagctac gcgagcggct gattgcggct cgggtcaccg
     4381 atgtcgcgga tgatgccgcg atcgccgcgg cgctcggaac cgatgatgtg gatgtcgcac
     4441 gcctgcgcgg cgcacttggc ccgtcgtccg atcagatcct gcgcgacatg atcgcgcacg
     4501 actgcgatgt cttcgtgcgc ctgctcgacg gctcgttccg cgaatatcag cgatccgcgc
     4561 agcggtggtg gaacgcgatt ctgccgagcg tgcctcaggg ccggccggtg tacttcgtgt
     4621 cgtcgaacag ccactcgctc gcgaacctgc tcggtggcta cgcgcgcgga caccgtgacg
     4681 agatcgtcgc gttcttgcgg cgccgcaact tcgagaatct cgcaccggcc tacgacgagg
     4741 ccatcgcggc cggcaaggaa gccgacgcga ccgcgatcct ctactactcg ctgcgcgcgt
     4801 tcattcacga gcccggcaac aacggccgca tggatctcgt gcagaagctc gacctcgagg
     4861 ccggcattcg cgcgatcggc tcgccgggca agatcgacgt cgacgcgcag gtgatcgacc
     4921 tcgccgagct caaggtcgag aacctcgatc cgcgcgtgtg cgtgccgggc atcgagaagc
     4981 tcgtcaacag ccgcgccgtc atcatcaaca tcgactaccc gctcggtatg gcgtcgtatc
     5041 accacctgtc ggcgatcgcg ctcggtgtcg acgatctgcg cggcatctac atcatgggca
     5101 aggccgcgac gctcaacggc cgcgtcggcg acgtcatgat cagcaaggtc gtgcacgacg
     5161 agcactcgcg caacacgtac ttgttcaaga acgcgctcgt cgccgccgac gtgcagccgt
     5221 tcatgcggca cggcaccgtg ctcgacaatc agaaggcgct gaccgtgcgg tcggcattct
     5281 tgcagaaccg cgagtacatg gacgtgttct accgcgaggg ctacacggtg atggagatgg
     5341 aggcggggcc gtacctctcg ggcgcgtacg agattgccga gccgcggcgc catccgcaga
     5401 acgagatcgt gtcgctcgtc gatcaatcga acttcgagct cggcgtcatc cactacgcgt
     5461 cggatacgcc gtactcgcga cgccagtcgc tgctctcgaa gagcttgtcg tacttcggca
     5521 tggagagcac gtacggctgc gcgatcgcga tcgcccggcg catcttcgcg aacgagctcg
     5581 cgaggcagac ttgaccgaca aggctgacgt cctcgtcatc ggcgctggcg tcagcgggct
     5641 cggatttgcg aactggtggc gcgagctgca ccccgacgcg cgcgtcgtgg tctgcgagtc
     5701 agaggcggac cccggcggct actgccgcac gatcgtgcgc gacggcttcg tctgggacta
     5761 ctccggccac ttctttcact tcaaagatcc gtcggtcgag cagtggctgc gcgcgaagat
     5821 gcccggcgag gacatccgca ccgtcgagcg cgtcgcgaaa atccgctacg ccggtcgcga
     5881 tatcgacttt ccatttcaga cgcacatcca tcagctgccg atcgacgagt tcctcgagtg
     5941 cctcacggcg ctgttctttc gccctacgag cgacgagccg ccacacagct tcggcgagat
     6001 gctgtaccgc cggctcggtg ccggcatcac cgacaagttc ctgcgcccgt acaacgagaa
     6061 gctgtacgcg acgaacctcg acacgctcga cgtcgacgcg atgggccggt tctttccgca
     6121 cgccgacatc gccgacatca tcggcaacat gcgtcccggg catcgcaagc acgggtacaa
     6181 cgcgacgttc acgtatccag cgggcggcgc ggttcgctac atcgaggcgc tgatgcgcgc
     6241 gttgccggcc ggcacggtgt cgtgcgacga gccgatcacc tcgatcgatc tcgacagccg
     6301 catcgcgacg acgccgaagc gtacgatcgc gttcgaccgg atcgtcagct cggcgccgct
     6361 gccggcgctc gcgtacatgt gcaagctcga gcacgacccg gccgtgttca cgtcgaacca
     6421 ggtgctcgtg ttcaatctcg gtttcgaccg caagggcatg cgcgacatcc actggatgta
     6481 ctttccggat ccggcgaccg tgttctatcg agtcggctgg tacgacaaca tcctcgacgg
     6541 cgatcgcatg agcctctacg tcgaaatcgg tgcggctgat ggcgcgacgt tcgaagtcga
     6601 tgcgctgcgc cagcgggtgc tcaccgacct cgcgcgagaa ggtatcgtga ccgatcacaa
     6661 gctcgtcgcc gagcatcacg tcgcgctcga tcccgcatac gtgcacatca cgcgcgcgtc
     6721 gctcgccgag acgaagcggt tgcgcgacct gctcgcgagc aaaggcgtgc actcggtcgg
     6781 ccgctacggc ggctggacgt actgctcgat cgaggacaac ttgatcgaga cgcgagcgct
     6841 tgcccgcagc ttgtagctag ggtgccggga tccgaacgac gacgtacttg cgatcgctga
     6901 gcgcgatcac gtcgatgacc ttgccgtcgg tgagcgccat cgcgggcgcg ctgcgccggt
     6961 cgtacacggg catcgcccga gcgggcaggg tgtagaacac cggcggtttg tcctcggccc
     7021 agacgccgac aacgccgtcg tgcgacgcga tcgcaacgag cttgcccttg atcgcagtga
     7081 cgttcgcgta ctcgggcgcg ccgctcggaa tcgtgaccgt tcggcacttg tcggtgcaga
     7141 tcgcgacatc gcgatgcgcc tcggggttac gcacgatcgc cgtgtcggcc gtgcagccct
     7201 gcagctcgta gaacgacggc agctgaacgc gatcgagtgg gcggccgccg ccgaacgcga
     7261 tcacctcgtg ctcgttgccc ggcagccaca cgcgatcggt cgtcaggcac ggttgtgcgc
     7321 cgaacgtcgc gggcagctcg aacgactctt cgccgcccgt cttgaacagc gtcgcgtggc
     7381 tcaccttgtc gggcgtcgtc cacacgagcg cgacacggcc gtcgaggtcg ctcgatgcca
     7441 gcgcgttgtc gatcttgagc gtcgtggccg gcgtgagcac gttcgccttt gcgtgcgcga
     7501 caacgagcga ctggaatgcg ccgtaaatga tcgtgccttc gttcagcgtg ccgctgacgg
     7561 ccgcgacggt caccgaacca atgcgctcgc cgcttttcag cgtcgggttg tcggagacca
     7621 tgttgcccgt cggcggaatc ggcgacgtga ccgcgagttt cgtcggggtg gcgatcacgc
     7681 cctcggcgtc catcgcgcct gcgagtagct cgccgacgct gtcgcgcttg ctgcccgtaa
     7741 ccttgagggt caggtcgccc gcggtcgcgc cccaggtcgc atccgggacc gagcgcatcg
     7801 aacccggacc gacgcgcgtg accgacgggt tgccaccggg cgaaaggctg atctggatct
     7861 ctcggctccc ggtcttgccg tacagcacga cgccgtgtgc ggagggcggc gaggtgacct
     7921 ccaccgaggt cacgggatcc ttgccgtcga cgagcggcac catctgcgcg gacggcagca
     7981 cggcgccggt ctgctggttt gccggcatct cggcaacgct gcgcagcttc gcacgtgcgg
     8041 cgtcgagcgc atcgagcgcg ccgggcagcg gatcctcggc gccggccgag ccgtcgacat
     8101 gctggacgaa cgcgagtgcg gtcttctgcg ccgccttgtc gaggtcattc cacgcggctt
     8161 cgacggcgtc gacgccggtg tcgtccccgt tcccgcgcga cagcttgctg acgatcggcg
     8221 tgcacttgcc gcggtcccac ggatcgggcg ccatctcgcg aatcgcgtgc gcttcgctcg
     8281 tcttcgccga acccggcgac tta
//
LOCUS       DASUFX010000281         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_460923,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000281 DASUFX010000000
VERSION     DASUFX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460923"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..968
                     /locus_tag="VFV99_11180"
     CDS             96..968
                     /locus_tag="VFV99_11180"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX5059916.1"
                     /translation="MEVKKLGVVGAGQMGQGIAQVAAQAGVDVIIVDAAPDFAQMGIG
                     KIKKTLDRLVERNKLDAAARDATMSHLKAGGGHRDLSECDVVIEAAPEKEELKLGIFK
                     SLGEVCRDDAILASNTSSISITKLATASGRPDRVIGMHFMNPVPLMKLVEIVRGLPTS
                     DETNTTVTDLAKRFGKTTIGARDIPGFIVNRMLIPLLNEACYGLYEGLGTAADIDTGV
                     KLGLNHPMGPLELADLIGLDTCLAIAEVLHRELGDDKYRPCPLLRQYVAAGWLGKKVL
                     RGFYEYDAAGQKKA"
     gene            973..1764
                     /locus_tag="VFV99_11185"
     CDS             973..1764
                     /locus_tag="VFV99_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5059917.1"
                     /translation="MSYPTFNTITIKQDGSLAIITINREAKLNALSSEVISELTQACA
                     ALEVSDEIRVVAVTGAGAKAFVAGADIAEMVELTANQAAAFAAGGHVLGEAIENSQNV
                     WVAAVNGFAFGGGCELALACDFVYAAENAKFGQPEVKLGVIPGFGGTQRLARRVGVAK
                     AKELCMTGDAIDAAEALRIGLVDAVFPQAELMGKVKALAERISANGPLAVAEVKRLIH
                     LGQSTTLDHALQLEQRSFGLLFATEDQREGMRAFLDKRKAQFKGK"
     gene            1761..2909
                     /locus_tag="VFV99_11190"
     CDS             1761..2909
                     /locus_tag="VFV99_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5059918.1"
                     /translation="MNFTPTEDQLAVLKMAREFAQNEVLPKAAEIDREHRHPAELVKR
                     MAELGFLGIAVPDQYGGAGFDHVSYALAMEEISRACASTGVIMSVNNSLVCDPIYRFG
                     TDAQKKEWLTPLASGKLLGCFALSEPEAGSDAAAQKTTAEKQADGTWVINGTKNWITN
                     GPVADVCVLFTMNDKAAGHKGITAFILPMKTKGVRCGSPDDKLGIRGSKSSQIFLDDV
                     RLPADAVLGDVGRGFAVAMSTLDGGRIGIAAQALGIARAALEDALAYAQQRRTMGKPI
                     IQHQAISFKLADMATEIDAARLLALRAAWLKDNKLPYGKEAAMAKLYASDVANRAARE
                     AIQIFGGNGYVTEFPVERHFRDAKITEIYEGTSEIQRLVIAGYLAKGG"
     gene            2913..3353
                     /locus_tag="VFV99_11195"
     CDS             2913..3353
                     /locus_tag="VFV99_11195"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5059919.1"
                     /translation="MKATLFLVVLAATACGGKNKNDANEGGGATIDTQATTGDPTDRS
                     GSMVPPEKMDEVNQLLGRKQMIISRCLSTAVESGEAPKGAKAKITLEISISPGGQVTK
                     AEVIKASLDVKSVQECVKGHVKEITFPTLPKQYETSYTYAMEAN"
     gene            3354..4193
                     /locus_tag="VFV99_11200"
     CDS             3354..4193
                     /locus_tag="VFV99_11200"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5059920.1"
                     /translation="MKRVVPLLLVACGGSKPPPPKEPPPPVEKKVTRVPIETNEDEPE
                     DGVTFTKQKGSISKEKIDAGLAPHQQAMMDCYTTKVGKRRFVGGEARLMWELKADGAI
                     KSVKLDSNLGAWDIEKCLLDVAWSATFDKPTGADAEFDLPLQFAATRSTAIWDEDMAL
                     RAVGGQLALLDECPNGDPLHKNKPGKKPPKKVAKKAPKKAPPPEAPAGPPERPEPRPP
                     SNVTVTMYIGPLGKAQSIGFSSPTSEVGSKWAKCASEVAASWRLPDPRGQIAKLAIRY
                     KAE"
     gene            4193..5341
                     /locus_tag="VFV99_11205"
     CDS             4193..5341
                     /locus_tag="VFV99_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5059921.1"
                     /translation="MQFAPTEEQLLVQRTARDFADRVLIPKAAARDVSCEFPLSEMKE
                     LGKLGLLGIAIPDELGGSGAGIIAYSLAMQELARGDASVAVAVSVTNMVGEMICRNGT
                     PEQQKRWNPDLASGAIVSGAFALSEADAGSDPGAMRTTATKTATGWRINGAKQWITTG
                     DQAGVMIVWAVTDPTAGHKGITAFIVPGKSKGLTVARLEDKLGLRASSTAQLVFDNVE
                     VGDDAVLGQPGQGFALAMMALDGGRIGIASQAIGIARGALEAAVRYAGERKTFGQPII
                     KHQAVGNMLADAATWLDAANLMTLRAAYLKETGQPFSQQAAMAKLFASEHGWKICDIA
                     LQVHGGYGYVKDFPVERALRDVRVTRIYEGTSEIQRIVIARNLLRGQS"
BASE COUNT         1053 a         1640 c         1848 g          813 t
ORIGIN      
        1 ctgcatcaga tggccgcgaa gaacgtgaag accggcggcg cgtcgctgtg tatcggcggc
       61 ggtgagggca tcgccctgct cgtggaacgc gcataatgga ggtcaagaag ctcggcgtcg
      121 tcggcgcggg ccagatgggg cagggcattg cgcaggtggc agcgcaggcc ggtgtcgacg
      181 tgatcatcgt cgacgccgcg cccgactttg ctcagatggg catcggcaag atcaagaaaa
      241 cactcgatcg tctcgtcgag cgcaacaagc tcgatgcggc ggcgcgtgat gcgacgatgt
      301 cccacctgaa agctggcggc ggtcaccgcg atctaagtga gtgtgacgtc gtgatcgagg
      361 ccgcacccga gaaggaagag ctcaagctcg gcatcttcaa gagcctgggc gaggtctgtc
      421 gcgacgacgc gattctggcg tcgaacacga gctcgatctc gatcacgaag ctggcgaccg
      481 cgtcgggcag acccgatcgc gtgatcggca tgcacttcat gaacccggtg ccgctgatga
      541 agctcgtcga gatcgtgcgc ggtctgccga cgagcgacga gacgaacacc accgtgaccg
      601 acctcgcgaa gcgcttcggc aagacgacga tcggcgcgcg cgacattccg ggcttcatcg
      661 tcaatcgcat gctgattccg ctcctgaacg aggcctgcta cggcctctac gagggcctcg
      721 gcaccgcggc agatatcgat acgggcgtga agctgggcct caatcacccg atgggtccgc
      781 tcgagctggc ggatctgatc ggtctcgata cgtgcctcgc gatcgccgag gtgctgcatc
      841 gcgagctcgg cgacgacaag taccggccgt gcccgctgct tcgccagtac gtcgcggcgg
      901 gttggctcgg caagaaggtg ttgcgcggtt tctacgagta cgacgcggcg ggtcagaaga
      961 aagcgtgaac cgatgagcta ccccacgttc aacacgatca cgatcaagca ggacggctcg
     1021 ctggcgatca tcacgatcaa ccgcgaggca aagctcaacg cgctatcgag cgaggtgatc
     1081 agcgagctga cgcaggcgtg cgccgcgctc gaggtctccg acgagattcg cgtggtcgcc
     1141 gtgaccggcg cgggtgcgaa ggcgttcgtc gccggcgccg acatcgccga gatggtcgag
     1201 ctcacggcga accaagcggc tgcgtttgcg gcaggcggcc acgtgctcgg tgaagcgatc
     1261 gagaacagcc agaacgtgtg ggtcgccgcg gtcaacggct ttgcattcgg tggcggctgc
     1321 gagctcgcgc tggcgtgtga cttcgtctac gccgcagaga acgcgaagtt cggccagccc
     1381 gaggtcaagc tcggcgtgat tcccggcttc ggcggcacgc agcggctcgc acgccgcgtc
     1441 ggcgttgcga aagcgaaaga gctgtgtatg accggcgatg cgatcgacgc agcggaagcg
     1501 ctgcgcatcg gccttgtcga cgcggtgttc ccgcaggccg agctcatggg caaagtgaaa
     1561 gcactcgccg agcgcatcag cgcgaacggg ccgttggccg tcgccgaggt gaagcggttg
     1621 attcacctcg gccagtcgac gacgctcgac catgctttgc agctcgagca gcgcagcttc
     1681 ggattactgt tcgcaaccga ggatcaacgc gaggggatgc gggcgttcct cgacaaacgc
     1741 aaagcgcagt tcaagggcaa atgaatttca cacccactga agatcagctc gcggtcctca
     1801 agatggcgcg cgagttcgcg cagaacgagg tgctgccgaa ggcagccgag atcgatcgcg
     1861 agcatcgcca tcccgcggag ctcgtgaagc ggatggccga gctgggcttc ctcggcatcg
     1921 cggtgcccga ccagtacggc ggcgccggct tcgatcacgt ctcgtacgcg ctcgcgatgg
     1981 aggaaatctc ccgcgcgtgc gcgtcgaccg gcgtgatcat gagcgtgaac aactcgctcg
     2041 tctgcgaccc gatctatcgg ttcggcaccg acgcgcagaa gaaggagtgg ctgacgccgc
     2101 tcgcgagcgg caagctgctc ggctgcttcg cgctcagcga gccggaagcg ggcagcgacg
     2161 cggccgcgca gaagacgacc gccgagaagc aggccgatgg gacgtgggtc atcaacggca
     2221 cgaagaactg gatcacgaac ggtccagtcg ccgacgtctg cgtgctgttc acgatgaacg
     2281 acaaggcggc aggccacaaa ggcatcaccg cgttcatcct gccgatgaag acgaagggcg
     2341 tccgctgcgg ctcgccggac gacaagctcg gtattcgcgg cagcaagtcg tcgcagatct
     2401 tcctcgacga cgtgcggttg ccggcggacg cggtgctcgg cgacgtcggt cgtggctttg
     2461 cggtcgcgat gtcgacgctc gacggtggcc gcatcggcat cgcggcgcag gcactcggta
     2521 tcgcgcgcgc cgcactcgag gacgcgctcg cctacgcgca gcagcgccgc acgatgggca
     2581 agccgatcat tcagcaccag gcgatcagct tcaagctggc cgatatggcg accgagatcg
     2641 acgcggcacg cctgctcgcg ctgcgcgcgg cgtggctcaa ggacaacaag ctgccgtacg
     2701 gcaaggaagc cgcgatggcg aagctgtatg cgtcggacgt cgcgaatcgc gcggcgcgcg
     2761 aagcgatcca gatcttcggc ggaaatggct acgtgaccga gttcccggtc gagcggcact
     2821 tccgcgatgc gaagatcacg gagatttacg aggggaccag cgagatccag cgtctggtga
     2881 tcgcgggcta ccttgctaaa gggggatgaa ccatgaaagc aacgctgttc ctcgtagtac
     2941 tcgcggctac cgcatgcggt ggcaagaaca agaacgacgc caacgagggt ggcggcgcga
     3001 cgatcgatac gcaggcgacg accggcgatc cgaccgatcg ctcgggtagc atggtgcctc
     3061 cggagaagat ggacgaggtc aaccagttgc tcggccgcaa gcagatgatc atttcgcgtt
     3121 gcctatcgac ggccgtcgaa tctggcgagg cgccgaaggg cgcaaaggcg aagatcacgc
     3181 tcgagatctc gatctcgccc ggcggccagg tcacgaaggc cgaggtcatc aaggcgtcgc
     3241 tcgacgtcaa gagcgtccag gagtgcgtga agggtcacgt caaagagatc acgttcccga
     3301 cgctgccgaa gcagtacgag acctcgtaca cgtatgcgat ggaagcgaac taaatgaagc
     3361 gcgtagtacc tctcttgctc gtcgcgtgtg gcggtagcaa accgccgccg cccaaggagc
     3421 caccgcctcc ggtcgagaag aaggtaacgc gcgttccgat cgagacgaac gaagacgagc
     3481 ccgaagatgg cgtcacgttc acgaagcaaa agggctcgat cagcaaagag aagatcgatg
     3541 ccggactcgc gccgcatcag caggcgatga tggactgcta cacgacgaag gtcggcaagc
     3601 gccgcttcgt cggaggcgag gcccggctga tgtgggagct caaggccgac ggcgcgatca
     3661 agagcgtcaa gctcgactcg aacctcggcg cgtgggacat cgagaagtgc ctgctcgacg
     3721 tcgcatggtc ggcgacgttc gacaagccaa ccggcgcaga tgccgagttc gacctaccgc
     3781 ttcagttcgc ggcgacgcgc tcgacggcga tctgggacga agacatggcg ctgcgcgccg
     3841 tcggtggcca gctcgcgctg ctcgacgagt gcccgaacgg cgatccgctg cacaagaaca
     3901 agcccggcaa gaagccaccg aagaaggtgg cgaaaaaggc gcccaagaaa gcgccgccac
     3961 cagaggcgcc cgctggtcca ccggagcgtc cggagccgcg gccaccgtcg aacgtgacgg
     4021 tgacgatgta catcggcccg ctcggcaaag cgcaatcgat cgggttctcg tcgccgacgt
     4081 cagaggtcgg aagcaagtgg gcgaagtgcg cgtcggaagt cgccgcgtcg tggcggttgc
     4141 cggatcctcg cggtcagatc gcgaagctcg cgattcgcta caaggcggag taatgcagtt
     4201 cgcgccgacc gaagagcagc tgttggttca gcgcacggcg cgcgacttcg ccgaccgcgt
     4261 gctgattccg aaagcggccg cgcgcgacgt ctcgtgcgag ttcccactca gcgagatgaa
     4321 agagctcggc aagctcggcc tgctgggcat cgcgattccc gacgagctag gcggttctgg
     4381 tgccggcatc atcgcgtact cgctcgcgat gcaagagctc gcacgcggcg acgcgtcggt
     4441 cgcggtcgcg gtcagcgtga cgaacatggt cggcgagatg atctgccgca acgggacgcc
     4501 ggagcagcag aagcgctgga accccgacct cgcgtcaggc gcgattgtgt cgggcgcatt
     4561 cgcgctgtcc gaggcagacg ccggttccga tccgggcgcg atgcgcacga ccgcgacgaa
     4621 gaccgcgacc ggctggcgca tcaacggtgc gaagcagtgg atcacgactg gcgatcaggc
     4681 gggcgtgatg atcgtgtggg cggtgaccga cccgacggct ggccacaagg gcatcacagc
     4741 gttcatcgtg ccgggcaagt caaagggcct gaccgtcgca cggctcgagg acaagctcgg
     4801 cctacgcgcg tcgagcacgg cacagctcgt gttcgacaac gtcgaggttg gcgacgacgc
     4861 agtcctcggc caacccggcc agggcttcgc gctggcgatg atggcgctcg acggcggtcg
     4921 catcggcatc gcgtcgcagg cgatcggcat cgcgcgcggc gcgctcgagg cggcggtgcg
     4981 ttacgcgggt gagcgcaaga cgttcggcca gccgatcatc aagcaccaag cggtcggcaa
     5041 catgctcgcc gacgcggcga cgtggctcga cgcggcgaac ttgatgacgc tgcgcgcggc
     5101 gtacctgaaa gagacggggc agccgttctc ccagcaggcc gcgatggcga agctgttcgc
     5161 gagtgagcac ggctggaaga tttgtgacat cgcgctgcaa gttcacggcg gctacggcta
     5221 cgtgaaggat tttcctgtcg agcgcgcgct gcgtgacgtg cgcgtcaccc gcatttacga
     5281 gggcacgagc gagatccaac gcatcgtcat tgcccgcaac ttgctgagag gtcagtcatg
     5341 agtgatcgca agct
//
LOCUS       DASUFX010000282        15330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1294223, whole genome shotgun sequence.
ACCESSION   DASUFX010000282 DASUFX010000000
VERSION     DASUFX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294223"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFV99_11210"
     CDS             <1..690
                     /locus_tag="VFV99_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059922.1"
                     /translation="ERALGAPIDDPERAQDDRIVIPSNFKNARVLMDEDAERIVGFVV
                     TSEPERSKEKEVAGEPRELLVRTSGDDFSVVTKDSEKLVATAKIPNLVFGRALRNPAD
                     GRDDFVAVTRTEDAQSRTWSLVAFRLHEGKLVRVIEAQPAELYKLTAANARWIGAELR
                     DLELYLELTARTDSIEVGGLLTTRIGDKIRDIVVISPVPVPRRKTKPVPHEPNDAGTY
                     DAASGSERAAE"
     gene            763..2580
                     /locus_tag="VFV99_11215"
     CDS             763..2580
                     /locus_tag="VFV99_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEX5059923.1"
                     /translation="MKFLCDKCKTRYSIGDERVRGKILKIRCKNCANVITVREGMAEP
                     DADAGARRNRSTSAQQAISATAAIEKVSLAPQGALGAAFASQMTKPPPALEEEWYVSM
                     DGNQEGPFSLPDAQRWVTGKPFDAELHCWSEGFDDWLPVDKVSHFRNLRKRPVVRPAT
                     IPPPIPKVHQPLFAATMASIEKAAAAPEKAAPPIPSTPAFKGAGAPLAAKANGTGSQL
                     PSLRGTGPGPAMPGQKPVSAGAAALAAAFGSDLGESHTTVDSPVFNDEIATTAEPHAA
                     KRIEAKASKPMMSPAVPVADSFQKPGVKPIMDDGGDDDDNLEIGEVSRVVKLTDIAAM
                     GRSKSTSNPAMRVGNATASNAALRPSASNIGRATGMTGAMTKLSPSELGMNVDPSLAA
                     LSPAGGPAPDESIVARSFASRHRRGLIVLIAVSAVMVLGVIAAVIFVVSGGNDDLPMG
                     LGGTRVIDTSRPEDIVRRQLPPPPNAGSAATTVPAHKKTTSRPGQSITTGPDEPETGP
                     GSHLDASEVEAMAAKYNEGTKRCYMRAQKGALGFEIAETKKIDVTITVGKDGSISDVK
                     LSKHANNEFGQCLISRIKGWKFRESKGGTFQFALAFSAV"
     gene            complement(2611..3750)
                     /locus_tag="VFV99_11220"
     CDS             complement(2611..3750)
                     /locus_tag="VFV99_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5059924.1"
                     /translation="MQPIYLDNAASTRVADEVLALMTEVMKTAWGNPSAQHPQGAAAR
                     GHIETAKKRMLEALGDKGMQWGDVVWTSGCSESNALAVLGAARTKPGRIVISTFEHPA
                     TSTLTERLAKEGREIVKVAPGPGGVLDPDEVARAAAGAGVVALIMVQNEIGVVQPVSA
                     IVQAIRAHNPSCHIHLDAAQALGKVALDVSTLGADSISIAGHKLHGPKGTGALWLRTS
                     AAIEPLWVGGGQQKGMRGGTQDAPGAAGLGLAAQRAVGALTTARAKWLELAGRVVTTL
                     TERGVTFRQLVPDHRRAPHILALGIAGVPASALRNVLASRGVYISTGSACADGESKAS
                     PVLEAIGLPADHGMVRLSFALDTSVDEVDTAAKIIADVARELRKS"
     gene            3770..4834
                     /locus_tag="VFV99_11225"
     CDS             3770..4834
                     /locus_tag="VFV99_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEX5059925.1"
                     /translation="MHYPWCRKLCPYCDFAVEVGTPEHEGYLAAILRELDSRAPSFSG
                     RLVSIYFGGGTPSLWRPDCLAQAIDAVCARFGAAREQLEITIEANPTDCDDTRLAAWR
                     GAGINRISIGVQSTAQDELVVLGRDHRFGDGPAAVARVVGFGGFTVTCDFILGVPPRK
                     QPAFITDVDHLSVYELTIEERTAFGQRVRAGRFTPLDEDALTELYVATDAELTAAGFE
                     HYEISSYAKPGKRAVHNSLYWRGAPFLGLGVGAASLWLAPDGGGERATNPRRAAEYMA
                     APGTPAERVAIPPAEMAADRAWLGLRTSDGVEEATLSTEIVAFLLDGGLGVRRAGRIC
                     PTLRGFLMADRIAARIIEAL"
     gene            4852..5892
                     /gene="hrcA"
                     /locus_tag="VFV99_11230"
     CDS             4852..5892
                     /gene="hrcA"
                     /locus_tag="VFV99_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5059926.1"
                     /translation="MGGNDLSRRAQKILHAVVTEYLQGGEAVGSRTVTRRHDLGLSPA
                     TVRNVMADLEEMGLLEQRHTSAGRVPTPTGLRFFIDSLLKVRGLTPREKDEIRERVIA
                     PSPDEVVQRASRLLSDLTHHAAVILAPDPDQQRLERIEFIPLRDGKLIAVLVTTDGRI
                     ENRLLLEDIEATRLDRIHNYLNQLLSGMTIDEVRDRVIREIAVDKNQYDEAVAMALRL
                     GHAVFVSPPERSASVLVAGQANLLDDVQPDRIRDLLRTLEDKETLVRLLDRTRQAEGL
                     QVFLGAETAMQALSGSSVVAMSYGPEEQPIGALAVIGPMRMNYGKVMSVVDFTADTVS
                     QLLAELARGGNS"
     gene            5914..6513
                     /locus_tag="VFV99_11235"
     CDS             5914..6513
                     /locus_tag="VFV99_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5059927.1"
                     /translation="MSGDDGQDDPKTPPEQKLPEEPSASGDVIPVEVEPPDAKDPVKE
                     LEEKLAAAEKEKKDNWDRYLRSAADLENTRKRQKRELEDAKFDIKNKVLKEMLPVVDN
                     LERAIEHAGGGGEKNPIVEGVQLVLRQFMTAFERLDVTVVDAMGQPFDPNLHEAISQQ
                     ESDQPAGTVIQVLQRGYRAGDRLLRPALVVVAKAKAQAE"
     gene            6510..8354
                     /gene="dnaK"
                     /locus_tag="VFV99_11240"
     CDS             6510..8354
                     /gene="dnaK"
                     /locus_tag="VFV99_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5059928.1"
                     /translation="MSRVVGIDLGTTNSCVALMDGDQGIVIANAEGTRTTPSVIGFAA
                     SGERLVGQAARRQAMTNAENTIYAVKRLMGRKFEDPEVQRHLLTCPYEVVAAPNGDAH
                     VRIRGRDYSPPEISAIVLQKMRQTAEDWLGEEVTEAVVTVPAYFDDAQRQATKDAGRI
                     AGLNVLRIVNEPTAAALAYGVEMQGAARVAVYDLGGGTFDISILELADGVFRVRATAG
                     DTFLGGEDFDNAVVEYMLDAFMQQNGMDLRGDRVALQRLKDAAERAKIELSSTDSTEI
                     NLPFLAAGSDGQPRHLIMQLSRGELENLVEPLVQATLEPCRRALEDAGFTADDIEVVI
                     LVGGQTRMPRVHQVVAEMFGKEASRRVNPDEVVAVGAAVQGGVLTGEVQEVLLLDVTP
                     LSLGVETMGGVFTRLIARNTSLPARATEIFSTTIDNQPFVNVHVLQGEREMADDNKSL
                     AHFELTGIPPAPRGVPKIEVAFDIDADGVLSVSARDHGTGRVQSVTVQPTTGLSDDDI
                     QRLVLESEQMQDDDAIRRLIIEARNKAEGLFYSSDKAMEEFGAMLDPVDRDQLLAELA
                     DCRAALQEEDLGIVEDAVARLEIAAQRIGEAIYAVADSSSGGDMGGGM"
     gene            8367..9272
                     /locus_tag="VFV99_11245"
     CDS             8367..9272
                     /locus_tag="VFV99_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5059929.1"
                     /translation="MARRKRDYYEVLGVERGATESDIKRAFRELARKHHPDVNPVDSG
                     EQFREINEAYAVLSDREQRARYDRWGHPDDGGSGFGAVVDAAQEMINEVLRRRRGKQK
                     GRDLRYTLEVTFEEAAFGASKTIKVPDEGAARDFTVVVPAGSKDGTVKTFKNEGERGK
                     GGAQPGDLHVIVRVAEHPQFKRDGVDVHSEHTIQFAQAALGAVIDLPTLDGPVKMRVP
                     EGTQPGRVFRIRGRGIPQASGKNAPRGDHLVHIQVEIPTELTPRQKQLIEELARERNE
                     GTGLLPSGEPAKKGLMDRVRSLLEE"
     gene            9288..10967
                     /locus_tag="VFV99_11250"
     CDS             9288..10967
                     /locus_tag="VFV99_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX5059930.1"
                     /translation="MERQAAKAAKNAKVRSFFFGVLGVLGTLALIWGCGPKVQTGTWV
                     ERDSELGRHSRHETESKAPSKAPPVHIDLSKLDPLTIDELDEATAIGVLEQLGDKKPA
                     ARVGLRAARLAHHRGDDADARALLARAASAADEAEVHADLGALAKATAAAPVDPNVVA
                     VLLPLSGRFQAIGAELRAAIELVPPDGSPTTPGAGVNPAPSISGPKGKAKWLFIDTKG
                     EPDGAAAAVDTAAQKGAVAILGPVGTREAVAAARAASLHGIPIALLAPSDGADPAAGV
                     FRLVSSPGDEARAVAKLAIDDNFPTVGVFAPRDDVGQDAADAFVAEATRLGVRVTAQG
                     TYDPTGGKLEPDVKQFLNLIPSKNPELAAHLRKNPKDGWKTFTPAVQFTLLYVPDRYD
                     RAAIVAAFLPYFGVELRTGESTDPNRLQRKHGGVIPQIVQLVGGAGWHHPSLPTRGGA
                     AVQGALIVDVFGAESGGDLAVAFNAAFQQKTGRAASSAAAEANDAATLVAQARFAAAA
                     AADPRTAFRSALAHGKLADGACGPAAIETDGELVRTPLILEVQGDELVPAP"
     gene            10992..11891
                     /locus_tag="VFV99_11255"
     CDS             10992..11891
                     /locus_tag="VFV99_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5059931.1"
                     /translation="MTRQDALIHGGLFLACCVTTTIAGALSSTLAGGIAFGATLMGIL
                     TCHELGHYFVGRKHRVDVSLPYFIPMPPYGTLGTLGAVIRMRKPIEDRGKLFDVGAAG
                     PIAGLVVAIPLLVIGLYLSDLAPQKPGDYVEGNSILYALLKYAVFGRWLPGDGLDVQL
                     HPMAFAGWVGLLVTMINLMPIGQLDGGHIAKSALGDSHEKWSARLHVALPIIGFTVGG
                     VMFVNALRGNETWYGALRYAGNGIIPWLVWTILLGWMRRNAGEYHPPVGETPLDPTRK
                     KLALGMLLLFVLIATPVPFRPVL"
     gene            11901..12263
                     /locus_tag="VFV99_11260"
     CDS             11901..12263
                     /locus_tag="VFV99_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059932.1"
                     /translation="MIDYKVRGAWQRVTETWSDSALHEELFKVAITDNCLAWVAARYR
                     ERGDDPIAKEQLERLRKAALAAMMVTAAERQAKEKTPYRSSFIVLGCLLVAMLVALFV
                     TKFIHDNGSPPATKAARP"
     gene            12279..12938
                     /locus_tag="VFV99_11265"
     CDS             12279..12938
                     /locus_tag="VFV99_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX5059933.1"
                     /translation="MAQVDFAERQLTLKLVYYGPPLSGKTTNLRALHAKVDKLNRGRL
                     MTLDTRDDRTLFFDLLPIFFRTSSFSFRIKVYTVPGQPVHEATRKVVLAGTDGVVFVA
                     DSSADQHQANRASWHNLVANLATFALDKVPIVVQYNKRDLPNAVPMTEVDRFGDPSRK
                     VLEAQATIGEGVVQTFFELVQASWEHLERDLQLAQKLKIDAQTFRRALAEHLGIAETT
                     G"
     gene            12978..14105
                     /locus_tag="VFV99_11270"
     CDS             12978..14105
                     /locus_tag="VFV99_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5059934.1"
                     /translation="MSVGGPLDGPIELARLVPPGELEELLGAVHRATGAVVAIYEPRS
                     KTRLAGADAAGAPRHPVEYNGELIAVLAATGDRADAGLELASDTLGLLVHHASARELA
                     ATMHEEAMRVTFAELTEHNNRLQRAVARLEELDRLKSNFLATMSHELRTPLTSVIGYA
                     EMMAEGLAGPITQEQRDYLATILSKADQLLGMITSVLDVAALETGPLALDRSRLSLAE
                     LVASEVAVYTSQAGKRGIAIQLESRGDTIVIGDKKKIRQIVSSLLSNAVKFTPDRGKV
                     GVAVRPGPLSPHETEELGAIQLVVSDSGIGIQRDHVGKIFEPFFQVDSSSTRAFGGTG
                     LGLTLAKAYVEAHGGRIWVDTSPGQGSTFVATFPLRVEETA"
     gene            14102..>15330
                     /locus_tag="VFV99_11275"
     CDS             14102..>15330
                     /locus_tag="VFV99_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5059935.1"
                     /translation="MIWLSQLSVAIPLAALCVLISGFCSGTEVALFSLRRVDREQLAR
                     SESALDRRILKLLERPRRLIATVLIGNEAFNSFLAVLALIVVARLVEVTSLWALAGIA
                     LGVSLPLIVLIAEVTSKTLAAKAPLTWARASVLPLSMFAVVVTPLRLVVNFIAEILLR
                     PLGDAARTRPVRDLSEQEFKSLIDASSAQGQVDARERRLIHRVFEFSDKNVGQVMTPR
                     DRIFAVSYDLSMQRLVKEVASRGFSRVPVYQKSLDNVRGIVNAKDLVNIAAGAPTRPL
                     NELLHEPLFVPRTTPVKRLFLTFKQKKVHMAIVVSEYGKVLGLVTMDDLLAQIFGVIR
                     DERAELQASNPNIKLPRARTPGVPSAVSSVESGPIPKQAILDTGPVTKEENLQRPDSG
                     NGNEALDPPPPPVRALFP"
BASE COUNT         2608 a         5039 c         5179 g         2504 t
ORIGIN      
        1 gagcgtgcgc tcggcgcgcc gatcgacgat ccggagcggg cgcaggatga ccgcatcgtg
       61 attccgtcga acttcaagaa cgcgcgcgtg ctcatggacg aagacgcaga gcgcatcgtc
      121 ggcttcgtcg tcacgagtga gccggagcgc agcaaggaaa aggaagtagc cggcgagccg
      181 cgcgagctgc tcgtgcgcac gtccggcgac gacttctccg tggtcaccaa ggacagcgag
      241 aaattggtcg cgaccgcgaa gattccgaat ctcgtgttcg gtagggcgct gcgcaacccg
      301 gccgacggcc gcgatgactt tgtcgcggtg acgcgtaccg aggacgcgca atcgcggacc
      361 tggtcgctgg ttgcatttcg cctgcacgag ggcaagctcg tgcgggtcat cgaggcccag
      421 ccggccgagc tctacaagct cacggccgcg aacgcgcgct ggatcggggc ggagctacgt
      481 gacttggagc tctatctaga gctgaccgcg cgcaccgatt ccatcgaggt cggaggcctt
      541 ctcaccacgc ggattggcga caagatccgc gacatcgtcg tgatctcgcc ggttcccgtg
      601 ccgcgccgca aaacgaagcc ggttccgcac gagcccaacg atgcgggaac ctacgatgct
      661 gcaagcggtt ctgagcgcgc cgctgagtga gtctacccct ttgcgagcac tggtattttt
      721 gggcacaatt gacgctcgcc gcgttgcggg acgcgttggg caatgaaatt tctctgcgac
      781 aagtgcaaga cccgatattc gatcggggac gaacgcgttc gcggcaagat cctcaagatc
      841 cgctgtaaga actgcgcgaa cgtcatcacc gttcgcgaag gaatggcgga accggatgcg
      901 gatgcaggag cacggcgcaa ccgctcgacg tcggcgcagc aggcgatttc cgcgaccgcc
      961 gcgatcgaga aagtctcgct cgcaccgcag ggtgcgctcg gcgctgcctt cgcgtcacag
     1021 atgacgaagc cgccgcccgc gctcgaagag gaatggtacg tgtcgatgga cggcaaccag
     1081 gaagggccgt tctcgcttcc cgacgcgcag aggtgggtga ccggcaagcc gttcgacgcc
     1141 gagctgcact gctggagcga aggcttcgac gactggctgc ccgtcgacaa ggtcagccac
     1201 ttccgcaatc ttcgcaaacg tcccgtcgtg cggccggcga ccattccgcc gccgattccc
     1261 aaagtccatc agccgctctt cgccgcgacg atggcgtcga tcgagaaagc cgctgctgcg
     1321 ccggagaaag ccgcgccacc aatcccgagc acgccggcgt tcaagggcgc aggtgcaccg
     1381 ctcgcggcca aggcgaatgg caccggctcg cagctaccgt cgctgcgcgg caccggtcca
     1441 gggcccgcga tgccgggaca gaaaccggtg agtgcgggcg cggctgcact cgctgctgca
     1501 ttcggaagcg acctgggcga gtcgcacacg accgtcgatt cgcccgtctt caacgacgag
     1561 atcgcgacga ccgccgaacc gcacgccgcg aagcgcatcg aggccaaggc gtccaagccg
     1621 atgatgtcgc cggccgtgcc ggtcgctgat tcgttccaga aacctggcgt caagccgatc
     1681 atggacgacg gcggcgacga cgacgacaac ctcgagatcg gtgaggtgtc gcgcgtcgtc
     1741 aagctcaccg acatcgcggc gatgggccgc agcaagtcga cgtcgaaccc ggcgatgcgc
     1801 gtcggcaacg cgacggcgtc gaatgctgcg ctgcggccga gcgcgagcaa catcggtcgc
     1861 gcgaccggca tgaccggcgc gatgacgaag ctatcgccga gcgagctcgg catgaacgtc
     1921 gacccgtcgc tcgccgcgct gtcgccggcc ggtggcccag cgcccgacga gagcatcgtc
     1981 gcccgctcgt tcgcgtcgcg gcatcgccgt ggtcttatcg tgctgatcgc ggtgtcggcc
     2041 gtgatggtcc tcggcgtcat tgcggccgtc atcttcgtcg tctcgggcgg taatgatgat
     2101 ctaccgatgg gcctcggcgg tacgcgcgtg atcgatacgt cgcgtcccga ggacatcgtg
     2161 cgcaggcagc tgccgccgcc gccgaacgcg ggctcggctg ccacgacggt gccggcgcac
     2221 aagaagacta cgtcccggcc gggtcagagc atcaccacgg gtcccgacga gccggagact
     2281 ggcccgggca gtcacctcga tgcgagcgag gtcgaggcga tggccgcgaa gtacaacgag
     2341 ggcacgaagc gctgctacat gcgcgcgcag aagggcgcgc tcggcttcga gatcgccgag
     2401 acgaagaaga tcgacgtcac gatcaccgtt gggaaggacg gctcgatcag cgatgtgaaa
     2461 ctgtcgaagc acgcgaacaa cgagttcggt cagtgcttga tctcgcgcat caagggttgg
     2521 aagttccgcg agtcgaaggg cggcacgttt cagttcgcgc tcgcattttc tgccgtctag
     2581 ctgccgtcta ggcagtcgtc gacgccagcg ctacgacttg cggagctcgc gcgcgacgtc
     2641 ggcgatgatc ttcgccgcgg tatcgacttc gtcgacgctc gtgtcgagcg cgaacgacag
     2701 gcgcaccatg ccgtgatcgg ccggtagccc gatcgcttcg agcaccggcg acgctttgct
     2761 ctcgccgtcc gcgcacgccg atcccgtcga gatgtacacg ccgcggctcg cgagcacgtt
     2821 gcgcagcgcg cttgccggca caccggcgat gcccagcgcg agaatgtgtg gcgcgcgccg
     2881 gtgatccgga acgagctggc ggaacgtcac gccgcgttcg gtcagcgtgg tgacgacgcg
     2941 gcccgcaagc tcgagccact tcgcacgcgc ggtggtcagt gcaccgacgg cgcgttgtgc
     3001 ggcgaggccg agaccagcgg caccgggcgc atcttgcgtc ccgccgcgca tgcccttctg
     3061 ttggccacca ccaacccaca gcggctcgat cgctgcgctc gtgcgcagcc acagcgcgcc
     3121 ggtgcccttc gggccgtgga gcttgtgacc ggcgatcgag atcgaatcgg cgccaagcgt
     3181 cgagacgtcg agcgcgacct tgccgagcgc ttgcgctgcg tcgaggtgga tgtggcagct
     3241 cgggttgtgc gcacggatcg cctgcacgat cgccgacacc ggctgcacga cgccgatctc
     3301 gttctgcacc atgatcagcg cgacaacgcc ggcacctgcc gcggcgcgcg cgacttcgtc
     3361 gggatcgagc actccgcccg ggccgggcgc gaccttcacg atctcgcggc cctccttggc
     3421 caagcgctcg gtgagcgtcg acgtcgccgg atgctcgaac gtcgagatca cgatgcggcc
     3481 aggcttcgtg cgtgcagcac cgagcacggc gagcgcgttc gactcgctac agccgctcgt
     3541 ccacacgacg tcgccccatt gcatgccctt gtcgccgagc gcttcgagca ttcgcttctt
     3601 cgcggtctcg atgtggccac gggcggcagc accctgcgga tgttgcgcgc tcgggttgcc
     3661 ccaggcggtc ttcatcacct ccgtcatcaa cgcgagcacc tcgtcggcga cacgagtcga
     3721 agcggcattg tcgaggtaga ttggctgcat ctccgatcta ggcgtctaca tccactaccc
     3781 gtggtgccgc aagctgtgcc cgtactgcga tttcgcagtc gaggtcggta cgcccgagca
     3841 cgagggctat ctcgctgcaa tcctgcgcga gctcgactcc cgtgcgccga gtttttctgg
     3901 ccggctggtc tcgatctact tcggcggcgg tacgccgtcg ctgtggcgac ccgactgcct
     3961 cgcgcaggcg atcgatgcgg tctgcgctcg gttcggtgcg gctcgggagc agctcgagat
     4021 cacgatcgag gcgaatccga ccgactgcga cgacacgcgg cttgcagcgt ggcgcggcgc
     4081 cggcatcaat cggatctcga tcggtgtgca atcgacggcg caggacgagc tcgtcgtgct
     4141 cggccgcgac caccggttcg gcgacggtcc agcggcggtc gcgcgcgtcg tcgggttcgg
     4201 cggcttcacg gtgacgtgcg acttcatcct cggtgtgccg ccgcgcaagc agcccgcatt
     4261 catcaccgac gtcgatcacc tctcggtgta cgagctgacg atcgaggagc gcaccgcgtt
     4321 cggccagcgc gtcagggcag ggcgcttcac gccgctcgac gaagacgcac tcaccgagct
     4381 gtatgtcgcg accgacgccg agctcaccgc cgctggcttc gagcactacg agatcagctc
     4441 gtacgcgaag cccggcaaac gcgccgtgca caactcgctg tactggcgcg gcgcgccgtt
     4501 tctcggcctc ggcgtgggcg cagcgtcgct gtggctagcg cccgatggcg gcggcgagcg
     4561 cgcgacgaac ccgcgccgag cggccgagta catggctgcg cccggaacgc cggccgagcg
     4621 tgtcgcgatc ccgccggccg agatggccgc ggaccgcgcg tggctcggcc tgcgcacgtc
     4681 ggatggtgtc gaagaagcca cgctctcgac ggagattgtc gcgtttctgc tcgacggcgg
     4741 gctcggcgtg cggcgtgccg gacggatctg cccgactctg cgcggctttc tgatggcgga
     4801 tcggatcgcg gcgcgcatca tcgaggcgtt gtaagctcgg atcgaatcgt catgggcggt
     4861 aacgacctat cgcgacgtgc gcagaagatc ctgcacgcgg tcgtcaccga gtacctgcaa
     4921 ggtggcgaag cggtcggctc ccgcacggtg acgcgtcgtc acgatctcgg tttgtcgcca
     4981 gcgacagtgc gcaacgtgat ggccgacctc gaggagatgg gtctgctcga gcagcgccac
     5041 acgtcggccg gccgcgtgcc gacgccgacg gggctgcggt tcttcatcga cagcttgctc
     5101 aaggtacgtg gcctgacgcc gcgcgagaag gacgagattc gcgagcgcgt gatcgcaccg
     5161 tcgcccgacg aggtcgtgca acgcgcgtcg cgcctcttgt cggatctcac gcatcacgcg
     5221 gccgtgatcc tcgcgcccga tcccgatcag cagcggctcg agcgcatcga gttcatcccg
     5281 ctgcgcgacg gcaagctgat cgccgtgctc gtcaccaccg acggccggat cgagaaccgc
     5341 ctcctgctcg aggacatcga ggcgacccgg ctcgatcgca tccacaacta tctgaaccag
     5401 ctgctctcgg gcatgacgat cgacgaagtg cgcgatcgcg tgattcgcga gatcgccgtc
     5461 gacaagaacc agtacgacga ggccgtggcg atggcgctcc gcctcggcca cgcggtgttc
     5521 gtctcgccgc ccgagcgctc ggcctcggtg ctcgtcgccg gtcaggcaaa cctgctcgac
     5581 gacgttcagc ccgaccgcat tcgcgatctg ttgcgcacgc tcgaagacaa ggagacgctg
     5641 gtgcgcctcc tcgatcggac gcggcaagcg gaggggcttc aggtgttctt gggcgccgag
     5701 accgccatgc aggcgctgtc gggcagctcg gtcgtggcga tgtcgtacgg tcccgaggag
     5761 cagccgatcg gtgcgctcgc tgtcatcggg ccgatgcgaa tgaactacgg caaggtcatg
     5821 tcggttgtcg atttcaccgc agacacagtt agccaactcc tcgcggagct ggcacgtggc
     5881 ggcaactcgt aggagttggt aagttccgcc gcaatgagcg gagacgatgg ccaagacgac
     5941 ccgaagaccc ctccggagca gaagctcccc gaggagccct cggcatcggg cgatgtgatc
     6001 cccgtcgagg tcgagccccc ggatgccaag gatccggtca aggagctcga agagaagctg
     6061 gcggcggcgg agaaggaaaa gaaagacaac tgggatcgct acctgcgctc ggcggccgat
     6121 ctcgagaaca cgcgcaagcg ccagaagcgc gagctcgaag atgccaagtt cgacatcaag
     6181 aacaaggtcc tcaaggagat gctcccggtc gtcgataacc tggagcgcgc gatcgagcac
     6241 gccggtgggg gcggcgagaa gaacccgatc gtcgagggcg tccagctcgt gctgcgccag
     6301 ttcatgaccg cgttcgagcg cctcgacgtt accgtcgtcg atgcgatggg ccagccattc
     6361 gatcccaacc tgcacgaagc gatctcccaa caggagagcg atcagccggc gggtacggtg
     6421 atccaggtac tgcaacgcgg gtatcgcgcg ggcgatcgcc tgctgcgccc ggcgctcgtt
     6481 gtcgtggcaa aggcgaaggc acaggctgaa tgagccgcgt cgtcggcatc gatctcggca
     6541 cgacgaactc ctgtgtcgcg ctgatggatg gcgaccaggg gatcgtgatc gcgaacgccg
     6601 agggtacgcg cacgacgccg tcggtgatcg ggttcgccgc gtcgggcgag cggctcgtcg
     6661 gtcaggcggc gcgtcgtcag gcgatgacga acgccgagaa cacgatctac gcggtgaagc
     6721 ggctcatggg ccgcaagttc gaggaccccg aggtgcagcg tcacttgctg acgtgcccgt
     6781 acgaggtcgt cgctgcgccg aacggcgacg cgcacgtgcg catccgcggt cgcgattact
     6841 cgccgccgga gatttccgcg atcgtgctgc agaagatgcg gcagaccgcc gaggattggc
     6901 tcggcgaaga ggtcaccgaa gcggtcgtga ccgtcccggc gtacttcgac gatgcgcaac
     6961 gccaggcgac gaaggatgcc ggccgcatcg ccggcctcaa cgtgctgcgc atcgtcaacg
     7021 agccgaccgc tgcggcgctc gcctatggcg tcgagatgca aggtgcagcg cgcgtcgcgg
     7081 tctacgacct cggcggtggc acgttcgata tctcgatcct cgagctcgcc gacggcgtgt
     7141 ttcgtgtgcg cgcgacggcg ggcgatacat tcctcggcgg cgaggatttc gacaacgcgg
     7201 ttgtcgagta catgctcgac gcgttcatgc agcaaaacgg catggacctg cgcggtgatc
     7261 gcgtcgcgct gcagcggctc aaggatgcgg ccgagcgcgc gaagatcgag ctgtcgagca
     7321 ccgactcgac cgagatcaac ctgccgttcc tcgcggcggg aagcgacggc cagccgcggc
     7381 acttgatcat gcagctgtca cgcggcgagc tcgagaatct cgtcgagccg ctcgtgcaag
     7441 cgacgctcga gccgtgtcgc cgcgcactcg aggatgccgg cttcaccgcg gatgacatcg
     7501 aggtcgtcat tctcgtcggc ggtcagacgc gtatgccacg cgtacatcag gtcgtcgccg
     7561 agatgttcgg caaggaagcg agccggcgcg tcaatcccga cgaggtcgtt gcggtcggcg
     7621 ccgcggtcca gggcggtgtg ctcaccggcg aagtgcagga agtgttgctg ctcgacgtca
     7681 cgccactctc actcggcgtc gaaacgatgg gcggcgtgtt cacgcgcctg atcgcgcgca
     7741 acacgtcgct gccggcgcgc gcgaccgaaa ttttctcgac gacgatcgac aaccagccgt
     7801 tcgtgaacgt gcacgtgctg caaggcgagc gcgagatggc agacgacaac aagtcgctcg
     7861 ctcacttcga gctcaccggc attccgccgg caccgcgcgg cgttccgaag atcgaggtcg
     7921 cgttcgacat cgatgccgat ggcgtgctca gcgtctcggc tcgcgatcac ggcaccggcc
     7981 gcgtgcagag cgtgacggtg cagccgacga cgggcctgtc ggacgacgac atccagcgcc
     8041 tcgtgctcga gtccgaacag atgcaggacg acgacgcgat ccgtcgcctc atcatcgaag
     8101 cccgtaacaa ggccgaaggc ttgttctatt cgtcggacaa ggccatggag gagttcggcg
     8161 cgatgctcga tcccgtcgac cgcgaccagc tcctcgccga gctggcggat tgccgggcgg
     8221 cgctgcaaga ggaggatctc gggatcgtcg aagacgccgt cgcgcgactc gagattgccg
     8281 ctcagcgaat cggtgaggcg atttacgccg tcgctgattc gtcttctggt ggcgatatgg
     8341 gcggcgggat gtaaagtcgc aactggatgg cgagacgcaa gcgcgactac tacgaggtgc
     8401 tcggtgtcga gcgcggcgcg acggaatccg atatcaagcg cgcgtttcgc gagctcgcac
     8461 gcaagcacca tccggacgtc aacccggtcg attcgggtga gcagttccgc gagatcaacg
     8521 aagcctacgc agtgctgtcc gatcgcgagc agcgcgcgcg ctacgatcgc tggggccacc
     8581 ccgacgatgg cggcagtggc ttcggtgcgg ttgtcgacgc cgcgcaggag atgatcaacg
     8641 aggtgctacg gcgccggcgc ggcaagcaga agggccgcga cctccgctac acgctcgagg
     8701 tcacgttcga agaagcggcg ttcggcgcga gcaagacgat caaagttccc gacgagggcg
     8761 ccgcgcgcga cttcaccgtc gtcgtgccgg ccggcagcaa agacggcacg gtcaagacgt
     8821 tcaagaacga gggcgagcgc ggcaaaggcg gtgcgcaacc gggcgacctc cacgtgatcg
     8881 tgcgcgtcgc cgagcatccg cagttcaagc gcgacggcgt cgatgtccac agcgagcaca
     8941 cgatccagtt cgcgcaggcg gcgctcggcg cagtgatcga tctgccgacg ctcgatggcc
     9001 cggtgaagat gcgcgtaccc gaaggcacgc agccgggccg cgtgttccgc attcgcgggc
     9061 gcgggattcc gcaggcgagt ggcaagaacg cgccgcgtgg tgatcacctg gtgcacatcc
     9121 aggtggaaat cccgaccgag ctcaccccga ggcagaagca gcttatcgag gagctcgcgc
     9181 gcgaacgaaa tgagggcacg gggctgcttc caagcgggga gccagccaag aagggcctca
     9241 tggatcgcgt ccgctccctg ctcgaggaat agggcgctcc tcctcagatg gaacgccaag
     9301 ctgccaaggc cgcgaagaac gccaaggtaa gaagcttctt ctttggcgtt cttggcgtac
     9361 ttggcacctt ggcgttgatc tggggatgcg gacccaaggt ccagaccggg acgtgggtgg
     9421 agcgcgactc cgagctcggc cgccattcga gacacgagac cgaatcgaag gcgccgtcga
     9481 aggcgccgcc agtgcatatc gatctatcca agcttgatcc gctgacgatc gacgagctcg
     9541 atgaggcgac tgcgatcggt gtcctcgaac aactcggcga taagaaaccg gccgctcgtg
     9601 tcgggctccg tgcggctcgg cttgctcatc accgcggcga cgacgccgac gctcgggcgt
     9661 tactcgcgcg cgcggcgagc gcagccgatg aagcggaggt tcacgccgac ctcggtgcgc
     9721 tcgccaaggc gacggccgcc gcgccggtcg atccgaacgt cgtcgccgtg ctgttgccgc
     9781 tgtcggggcg gttccaggcg atcggcgccg agctgcgcgc tgcgatcgag ctcgtgccgc
     9841 cagatggctc gccgactacc ccgggggccg gagtgaatcc ggccccctcc atttcggggc
     9901 cgaagggcaa agcgaagtgg ctgttcatcg ataccaaggg cgagccagac ggcgcggccg
     9961 cagctgtcga caccgcggcg cagaagggcg cggtcgcgat cctcggcccg gtcggcacgc
    10021 gcgaggcggt ggcagctgct cgcgcggcat cgctgcacgg cattccgatc gcgctgctcg
    10081 caccatccga tggcgcggat ccggcggcag gcgtgtttcg cctcgtcagc tcgcccggtg
    10141 acgaagcgcg cgcggtggcg aagctcgcga tcgacgacaa cttcccgacg gtcggcgtgt
    10201 tcgcgccgcg cgatgacgtc ggccaggacg ccgcggacgc gttcgtcgcc gaagcgacgc
    10261 ggctcggcgt tcgcgtcacc gcgcagggca cgtacgatcc gacgggcggc aagctcgagc
    10321 ccgacgtcaa acagttcttg aacctgatcc cctcgaagaa cccggagctc gccgcgcacc
    10381 tgcgcaagaa tccgaaggac ggctggaaga cgttcacgcc agccgtgcaa ttcacgctgc
    10441 tctacgtgcc ggatcgctac gaccgcgccg cgatcgtcgc tgcgttcctg ccgtacttcg
    10501 gcgtcgagct gcgcaccggc gaatcgaccg atcccaatcg tctgcagcgc aagcacggcg
    10561 gcgtgatccc gcagatcgtt cagctcgtcg gcggtgcggg ctggcatcac ccgagcttgc
    10621 cgacgcgcgg tggtgcggcg gtgcagggtg cgttgatcgt cgacgtcttc ggtgccgaga
    10681 gcggtggcga tctcgccgtc gcgttcaatg cagcgttcca gcaaaagacc ggtcgcgccg
    10741 cgagcagcgc cgctgccgag gcgaacgatg ccgcgacgct cgtcgcgcag gcgcgcttcg
    10801 ctgccgcggc tgccgccgat ccacgaacgg cgtttcgcag cgcactcgcg cacggcaagc
    10861 tcgccgacgg cgcgtgcgga ccagccgcga tcgagaccga cggcgagctc gtacgaaccc
    10921 cgctgatcct cgaagtccaa ggcgacgagc tagtcccggc gccgtaggca gcgatcggct
    10981 agactcgtgg cgtgacgcgg caggatgcgt tgatccatgg cggcttgttc ctcgcctgct
    11041 gcgtgacgac cacgatcgcc ggcgcgttgt cgtcgacgct cgcaggtggc atcgcgttcg
    11101 gtgcgacgtt gatgggcatc ctcacgtgtc acgagctcgg ccactacttc gtcggtcgca
    11161 agcatcgggt cgacgtgtca ctgccgtact tcatcccgat gccgccgtac ggcacgctcg
    11221 gcacgctcgg cgcggtgatt cgcatgcgca agccgatcga agatcgcggc aagctgttcg
    11281 atgtcggcgc ggcaggaccg atcgcggggc tcgttgtcgc gattccgctg cttgtcatcg
    11341 ggctctactt gtccgacctc gcgccccaga agccgggcga ctacgtcgag ggcaactcga
    11401 tcctctacgc gctgctcaag tacgccgtat tcgggcgctg gctgcccggc gacggtctcg
    11461 acgtccagct ccaccccatg gcgtttgccg gttgggtcgg gctgctcgtc acgatgatca
    11521 acctgatgcc gatcggtcag ctcgacggtg gccacatcgc gaagtctgcg ctcggcgaca
    11581 gccacgagaa gtggtccgcg cgtctgcacg tcgcgctgcc gatcattggc ttcacggtcg
    11641 gcggcgtgat gttcgtcaat gcgctccggg gtaacgagac ctggtacgga gcgctacgct
    11701 acgcaggtaa cggcatcatt ccgtggctgg tctggaccat cttgctgggg tggatgcgtc
    11761 gaaatgcggg cgagtaccac ccgcccgttg gggagacgcc gctcgatccg acgcggaaga
    11821 agctcgcgct tggcatgctc cttttgttcg tgctgatcgc gacaccggtg ccgtttcggc
    11881 ccgtgctgta aactggtgat gtgatcgact acaaggtgcg tggcgcgtgg caacgggtca
    11941 ccgagacctg gagcgactcg gcattgcacg aggagctgtt caaggtcgcg atcacggaca
    12001 actgcctcgc gtgggtggcg gcccgctacc gcgagcgtgg cgacgatccg atcgccaagg
    12061 agcagctgga gcggctccgt aaggcggcgc tggcggccat gatggtcact gcggccgaac
    12121 gccaggcgaa ggaaaagacg ccgtaccgca gttctttcat cgtgctcggc tgcctgctcg
    12181 tcgcgatgct ggtggcgctg ttcgtgacca agttcattca cgacaacggg tcgccgccgg
    12241 ccaccaaagc tgcacgtccc tgagctagag ttggtttcgt ggcccaagtc gactttgctg
    12301 agcgtcagct gacgctgaag ctcgtgtatt acgggccgcc gctatcgggg aagacgacga
    12361 acctgcgtgc gctgcatgca aaggtcgaca agctcaaccg cgggcgcctg atgacgctcg
    12421 acacgcgcga cgaccgcacg ctgttcttcg acctgctacc gatcttcttc cggacgtcga
    12481 gcttctcgtt ccgcatcaag gtctacacgg tgcccggtca gccggtgcac gaggcgacgc
    12541 gcaaagtcgt gctcgccggt accgacggcg tcgtgtttgt cgccgactcg agcgccgacc
    12601 agcaccaagc gaatcgcgcg tcgtggcaca acctcgtcgc gaacctcgcc acgttcgcgc
    12661 tcgacaaggt gccgatcgtc gtccagtaca acaagcgcga cctgccgaac gctgtaccga
    12721 tgaccgaggt cgatcggttt ggcgatccga gtcggaaggt tttggaggcg caggcgacga
    12781 tcggcgaggg cgtggttcag acattcttcg agctggtaca ggcctcctgg gaacacctgg
    12841 agcgggacct acagctcgcc cagaagctca aaatcgatgc gcaaacgttt cgccgcgcgc
    12901 tcgcagagca cctcggcatc gcagaaacca ctggttagac tgcggtttag ccgcctggga
    12961 tgcgctagaa tggggccgtg agcgtcggag gaccgctcga tggtccaatc gagttagctc
    13021 gcctggttcc gcccggtgag ctcgaagagc tcctcggcgc ggtccatcgg gcgacgggag
    13081 cggtcgtcgc gatctacgag ccgcggagca aaacgcgact ggccggcgcc gatgctgccg
    13141 gcgcgccgcg tcatccagtc gagtacaacg gcgagctgat cgcggtgctc gcagcgaccg
    13201 gcgatcgcgc tgacgccggg ctcgagctcg caagcgacac gctcgggctg ctcgtccatc
    13261 acgcgagcgc gcgcgagctc gccgcgacga tgcacgaaga ggcgatgcgg gtgacgttcg
    13321 ccgagctgac cgagcacaac aatcgactcc agcgcgcggt cgcccggctc gaagagctcg
    13381 atcgattgaa gtcgaacttc ctcgcgacga tgtcgcacga gctgcgcacg ccgctgacca
    13441 gcgtgatcgg ctacgccgag atgatggccg aggggctcgc cggtccgatc acgcaggagc
    13501 agcgcgacta cctcgcgacg atcctcagca aggccgatca gctgctcggc atgatcacca
    13561 gcgtgctcga cgtcgcggcg ctcgaaaccg ggccgctcgc gctcgaccgc agccggctct
    13621 cgctggccga gctggtcgcg agcgaggtcg ctgtctacac ctcgcaggca ggcaagcgcg
    13681 ggatcgcgat ccagctcgaa tcacggggcg acacgatcgt catcggcgac aagaagaaga
    13741 tccgccagat cgtgtcgagc ctgttgtcga atgcggtcaa gttcacgccc gatcgcggca
    13801 aggtgggcgt cgccgtgcgc cccggtccgc tgtctccgca cgaaacagag gaactgggcg
    13861 cgatccagct cgtcgtcagc gactcgggca tcgggatcca gcgcgaccac gtcggcaaga
    13921 tcttcgagcc gttcttccag gtcgattcgt cgtcgacgcg cgcgttcggc ggcaccggtc
    13981 ttggcctcac gctcgcgaag gcctacgtcg aggcgcacgg tggccggatc tgggtcgaca
    14041 cctcaccggg acaaggctcg acgttcgtcg cgacgttccc gcttcgcgtc gaggagacgg
    14101 cgtgatctgg ctcagtcagc tttccgtcgc catcccgctc gctgcactgt gtgtgctcat
    14161 ctccgggttc tgctcgggga ccgaggtcgc gctgttctcg ttgcgccgcg tcgatcgcga
    14221 gcagctcgcg cgcagcgaat ccgcgctcga ccgccgcatc ctcaagctgc tcgagcggcc
    14281 gcgccgactg atcgcgaccg tcttgatcgg taacgaggcg ttcaacagct tcctggccgt
    14341 gctcgcgctg atcgtcgtcg ctcggcttgt cgaggtcaca tcgctgtggg cgctcgccgg
    14401 catcgcgctc ggcgtatcgt tgccgttgat cgttctgatc gcagaggtga cgagcaagac
    14461 gctcgcggcg aaggcgccgc tcacctgggc tcgcgcatcc gtgttgccgc tgtcgatgtt
    14521 cgcggtcgtc gtcacgccgc tgcgcctcgt cgtgaatttc atcgccgaaa tcctgctgcg
    14581 cccgctcggt gatgccgcgc gcacgcgccc ggtacgcgat ctctccgagc aagagttcaa
    14641 gtcgctgatc gacgccagct cggcgcaggg ccaggtcgac gcgcgcgagc gccggctcat
    14701 tcaccgcgtc ttcgagttct ccgacaagaa cgtcggccag gtgatgacgc cgcgcgatcg
    14761 catctttgcc gtcagctacg acctctcgat gcagcggctc gtgaaggagg tcgcgtcgcg
    14821 cggcttctcg cgcgtgccgg tctatcagaa gtctctcgac aacgtgcgtg gcatcgtcaa
    14881 cgcaaaggat ctcgtcaaca tcgccgccgg tgcaccgacg cgtccgctca acgagctgtt
    14941 gcacgagccg ctgttcgttc cgcgcacgac gcccgtcaag cgcttgttct tgaccttcaa
    15001 gcagaagaag gtccacatgg cgatcgtcgt cagcgagtac ggcaaggtgc tcggcctcgt
    15061 cacgatggat gatctgctcg cgcagatctt cggtgtgatt cgcgacgagc gcgcagagct
    15121 gcaggcgagt aacccgaaca tcaagctgcc gcgcgctcgc acgcccggcg tgccgagtgc
    15181 ggtgtcctcc gtcgagtccg ggccgattcc gaagcaggcg atcctcgaca cgggtccggt
    15241 gacgaaggag gagaacctcc agcgtcctga cagcggcaat ggcaacgagg cgctcgatcc
    15301 gccgccaccg ccggtgaggg cgctgttccc
//
LOCUS       DASUFX010000283         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_738821,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000283 DASUFX010000000
VERSION     DASUFX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738821"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFV99_11280"
     CDS             <1..510
                     /locus_tag="VFV99_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5059936.1"
                     /translation="TNQVELALDAIQQDISQSVMLNTRLGWDTHDTNTDQAGFHETTF
                     AGLMRLVDGLAARPGRKAGTTMLDDTVVVCYSEFSRTPKLNSQLGKDHWPVTSAMVIG
                     AGVKGGRGYGASTAAVEADTVDFSTGDRSATGMTLMSNHFCAGVLKACGIDPIAHLGP
                     TEVFDAFVA"
     gene            494..880
                     /locus_tag="VFV99_11285"
     CDS             494..880
                     /locus_tag="VFV99_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059937.1"
                     /translation="MRSWLSLALVLAACGTEVPAGDGSGSGSGSDDEQVDPSVCDTSY
                     LDYDNFGAPFVINWCRGCHSDAVPAGMRQKAPLDANFDTLAQVRTWSQKIATRATGTT
                     PNMPPAGGPTEQERQLLAEWLACGAK"
     gene            complement(1078..1623)
                     /locus_tag="VFV99_11290"
     CDS             complement(1078..1623)
                     /locus_tag="VFV99_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059938.1"
                     /translation="MKVFLSYALSLGGLGMVIAAVLGAYVVNLSYPAARLRMLNMLRT
                     SVNQAEVACRAAKGTFYEPIGAAIKIGAMVPTTDLNILRQTTTPGYDAACMQVKLHWK
                     KLFGRGKKGAAMVVGGLALAIASKAMPALHIICVVLMVGGAVWFFVTMTENERSLVRA
                     KAELLPELDRAFAEGRYQRVG"
     gene            complement(1637..2584)
                     /locus_tag="VFV99_11295"
     CDS             complement(1637..2584)
                     /locus_tag="VFV99_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5059939.1"
                     /translation="MSFTPRGFRLLRIATIAIVAVLLVLFLRNLDWDKLAHALRHASL
                     VPLLLSSVIYFVCLFGKALSWRIMLQPRNVVSIWRLYRYTIAAFAASALTPARAGELL
                     RIWALKARDNVPVFDSTGVAIAEKLLDAVTLLLVCAPAPFLMPQLPGWVTNAMLACGG
                     IAVAVLLVLFFAVGRIEAREPRSWFGRMIAGMNVVRDPKRMAQAILVLVLTWCADLIA
                     VVLVLHAVGIDVPIGAAMFILFTFNLAVAIPAAPGQVGTLQVGALAATSVLGIPEEPA
                     LAFALLYQAMQLAPLLILAFVLELDLMRGRTRPEPQPAS"
     gene            complement(2618..3631)
                     /locus_tag="VFV99_11300"
     CDS             complement(2618..3631)
                     /locus_tag="VFV99_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5059940.1"
                     /translation="MDVIDAIGKTPLIELKKVTAGLKNRVFIKCEHLNPGGSVKDRTA
                     RAIILDAEARGVLVPHSDPPMTIIEGTAGNTGVGLALLASARGYRCICVIPEKVAADK
                     RVALRQLGAEVYIAKAAGIKSPDNFRNVAARLAEENGWFLTNQFHNPANLDAHYGGKG
                     FSATGPEIYEQMGGNLAAFVSSAGTGGTISGVGKYLKEKNPAIKVVLTDPYGSGLGEW
                     VNTGEVGPDGPFVIEGIGSASVPSNLLREPLDWAEKVTDAEALAMVERLVKEEGLTVG
                     GSTGVNVVGALRVAARADVNGPVVTIAADLWDRYRSTQWMKEWEAREAAGLVFMQPHQ
                     PKP"
     gene            3706..4356
                     /locus_tag="VFV99_11305"
     CDS             3706..4356
                     /locus_tag="VFV99_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059941.1"
                     /translation="MRGLLVVAVVVGWALPAAADPAKPVCQRAAQTVTVLIKASDKAQ
                     TLIGRRCEKDRWSRDAQACFAGSGSSVDAQRCLDKLSKDQRTQLEGDADRLNDSKLTQ
                     WLAKRVMVARALPPPTITLAVLDETDLRQARLLQQQGMSAYQAGRYDYAVRKFSAALD
                     TSPTPELLYHAAQAYRLKGDRAKAIELYEKYLDVSPRGPAANASRAELEKLRDAVP"
BASE COUNT          817 a         1505 c         1454 g          729 t
ORIGIN      
        1 acgaaccagg tcgagctcgc gctcgacgcg atccagcaag acatctcgca gtcggtgatg
       61 ctcaacactc gcctcggttg ggacacgcac gacacgaaca ccgaccaggc cggcttccac
      121 gagacgacgt tcgccggtct gatgagactg gtcgacgggc tcgccgcgcg ccctggccgt
      181 aaggccggca cgacgatgct cgatgacacg gtcgtcgtct gttactcgga gttctcgcgt
      241 acgccgaagc tcaactcgca gctcggcaag gatcactggc cggtgacatc ggcgatggtc
      301 atcggcgccg gcgtcaaagg cgggcgtggc tacggcgcga gcacggcggc cgtcgaagcc
      361 gacacggtcg acttctcgac cggcgaccgc agcgcgacgg gcatgacgct gatgtcgaac
      421 cacttctgcg ctggcgtgct gaaggcgtgc ggcatcgatc cgatcgctca tctgggcccg
      481 acggaggtgt tcgatgcgtt cgtggcttag tctcgcgctc gttctcgcgg cgtgtggcac
      541 tgaagtcccg gcaggcgacg gctcgggctc gggctccggc tccgacgacg agcaggtcga
      601 cccgagtgtc tgcgacacct cgtatctcga ctacgacaac ttcggcgcgc cgttcgtgat
      661 caactggtgc cgcggctgtc actcagacgc ggtacccgcc ggcatgcgcc agaaggcgcc
      721 gctagatgcg aacttcgaca cgctcgcgca ggtgcggacg tggagtcaga agatcgcgac
      781 tcgcgcgacc ggaacgacgc caaacatgcc gccggccgga ggcccgaccg aacaagagcg
      841 ccagctgctc gccgaatggc tcgcctgcgg cgcgaagtaa ctgctcagtg gtggccgccc
      901 cactggtgcc ggaggaacag caccatcgcg acgatgataa cgacggcgat cacgaacgtg
      961 tgcaggatgt acgtctgagg aggcttgtgg tccgcgctca cgtggcgaga ctacttcacg
     1021 aaccgagctt gcggtaggtc gtcgagcacg atcgtgtcgt ggcgcgtcga ggacggatca
     1081 gccgacgcgc tgatagcggc cttcagcgaa tgcacgatcg agctcgggca acagctcggc
     1141 cttcgcgcgc accagcgagc gctcgttctc ggtcatcgtc acgaagaacc acaccgcgcc
     1201 gccgaccatg agcacgacgc agatgatgtg tagtgcaggc atcgccttgc tcgcgatcgc
     1261 gagcgcgagc ccacctacca ccatcgcggc gcccttcttg ccgcggccga acagcttctt
     1321 ccagtgcagc ttcacttgca tgcacgcagc gtcgtagcca ggcgtcgtcg tctggcgcag
     1381 gatgttgagg tccgttgtcg gcaccatggc gccgatcttg atcgcagcgc cgatcggttc
     1441 gtagaacgtg cccttcgcag cgcggcacgc gacctccgcc tggttcacgc tcgtgcgcag
     1501 catgttgagc atgcgcaggc gcgcagctgg atagctcaag ttcaccacgt aggcgccaag
     1561 cacagcggcg atgaccatgc cgaggccgcc gagcgacaac gcatagctga ggaacacctt
     1621 cactacagcg agtgtactac gaagccggct gcggctccgg tctcgttcga ccgcgcatca
     1681 gatcgagctc gagcacgaag gcgaggatga gcaggggcgc gagctgcatc gcttgataaa
     1741 gcaatgcaaa cgcgagcgct ggctcctcgg ggatcccgag cacgctggtc gcggcgagcg
     1801 caccgacttg cagcgtcccg acctggcccg gcgccgccgg aatcgcgacc gcgaggttga
     1861 acgtgaacag gatgaacatc gcggcgccga tcggcacgtc gatgccaacc gcgtggagca
     1921 cgagcaccac cgcgatcaga tcggcgcacc acgtcagcac gagcacgagg atcgcctgcg
     1981 ccatccgctt cggatcgcgg acgacattca tgccggcgat catccgcccg aaccacgagc
     2041 gcggctcgcg ggcctcgatg cggccgactg caaagaacag cacgagcagc actgcgactg
     2101 cgatcccgcc gcacgcgagc atcgcgttcg tgacccagcc ggggagctgc ggcatcagaa
     2161 acggcgccgg tgcgcacacg aggagcagcg tgaccgcgtc gagtagcttc tcggcgatcg
     2221 cgacgccggt cgaatcgaag accggcacgt tgtcgcgcgc tttcagtgcc cagatgcgga
     2281 gtagctcgcc ggcgcgtgcg ggcgtcagcg ccgaggccgc gaacgccgcg atcgtgtagc
     2341 ggtacagccg ccagatcgac acgacgttgc gcggctggag catgatccgc cacgacagcg
     2401 ccttgccgaa caagcagacg aagtagatca ccgacgacag cagcagcggt acgagcgacg
     2461 cgtggcgcag cgcatgcgcg agcttgtccc aatccagatt gcgcaggaac agtacgagca
     2521 acaccgcaac gatcgcgatc gtcgcgatcc gcagcaggcg gaaaccgcgc ggcgtgaagc
     2581 tcactactga aggctagcgt gcgtgcccgc gctgtctcta aggcttcggc tgatgcggct
     2641 gcatgaacac gagcccagct gcctcacgcg cttcccactc tttcatccac tgcgtcgagc
     2701 gatagcgatc ccacaggtct gcagcaatcg taacgaccgg accgttcaca tccgcacgtg
     2761 cggcaacgcg cagtgcgccc accacgttga cacccgtgga gccaccgacc gtgagtcctt
     2821 cttctttgac cagtcgctcg accattgcga gcgcctcggc gtcggtgact ttctcggccc
     2881 aatcgagcgg ttcgcgcaac aggttcgacg gcacgctcgc cgacccgatg ccttcgatca
     2941 cgaacggacc atcgggaccg acctcgccgg tgttcaccca ttcgccgaga cccgagccgt
     3001 atggatcggt gagcacgacc ttgatggctg ggttcttctc cttgaggtac ttgccgacgc
     3061 cgctgatcgt gccgccggtg cccgcgctcg acacgaacgc cgcgaggttg ccgcccatct
     3121 gctcgtagat ctcgggcccc gtcgccgaga agcctttgcc gccgtagtgc gcgtcgaggt
     3181 tcgccggatt gtggaactgg ttcgtgagga accagccgtt ctcctctgcg agccgcgcgg
     3241 cgacgttgcg gaaattgtcc ggactcttga tgccggccgc cttcgcgata tagacctcgg
     3301 cgccgagctg acgcagcgcg acgcgcttgt cggccgccac cttctccgga atcacacaga
     3361 tgcagcgata cccgcgcgcc gacgcgagca gtgcgaggcc aacgccggtg ttgccggcgg
     3421 tcccctcgat gatcgtcatc ggcggatcgg agtggggcac gagcactccc cgcgcctcgg
     3481 catccaggat gatcgcgcgc gccgtacggt ctttcacgct gccgcccggg ttcaagtgct
     3541 cgcacttgat aaacacgcgg ttcttcaggc cggcagtgac cttcttcagc tcgatcagtg
     3601 gggtcttacc gatcgcgtcg ataacgtcca ttgcggcccg accatagcaa gccgcgtcgg
     3661 tttccgttgc cttccgcgca tgcacgggcg tatttatcgc cccgcatgcg agggcttctg
     3721 gtcgttgcgg tcgtcgtggg gtgggcattg cccgcggctg ccgatccggc caagccggtc
     3781 tgtcagcgcg ccgcccagac ggtgaccgtg ctgatcaagg cgagcgacaa ggcacagacg
     3841 ctgatcggtc gccgctgcga aaaagatcgc tggtcgcgtg acgcgcaggc gtgcttcgcc
     3901 ggctccggtt cgagcgtcga tgcgcagcgc tgtctcgaca agctcagcaa ggaccagcgc
     3961 acgcagctcg aaggcgatgc ggatcgcctc aatgacagca agctcaccca gtggctcgcg
     4021 aagcgggtga tggtcgcgcg tgcattgcca ccgccaacga tcacgctcgc ggtgctcgac
     4081 gagacggacc tgcgtcaggc gcggttgctc cagcagcaag gcatgtcggc atatcaagcc
     4141 ggtcgctacg actacgccgt ccgcaagttc tccgcggcac tcgatacgag cccgaccccg
     4201 gagctcctct atcacgcggc gcaggcgtat cgcctcaagg gcgatcgcgc gaaagcgatc
     4261 gagctgtacg agaagtatct cgacgtatcg ccgcgcggcc cggcagcgaa cgcatctcgt
     4321 gcagagctcg agaagctgcg cgacgctgtg ccctaggagc gtgtagctcg tagaggtccg
     4381 cgacgagctg agctgacttc gtgcccacgt gggcacggac ctcaagcagc ggctgcgatt
     4441 cgaggccgcc gtccgagagc tcgccacagc ttcaggacgt tgtgggtggc gcaaatcagt
     4501 agcca
//
LOCUS       DASUFX010000284         8715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1432184, whole genome shotgun sequence.
ACCESSION   DASUFX010000284 DASUFX010000000
VERSION     DASUFX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432184"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFV99_11310"
     CDS             <1..925
                     /locus_tag="VFV99_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059942.1"
                     /translation="VMTAPLFTRQNAIEVTFNIALEPDTAAIDTGLADEPLANLSAIV
                     SGHAHYDHFMDVPHILTKAPNATAYTNLTGRHVLAALAPDRPSCANATPSPELPRDRI
                     VAMDDPLASHVDYTNCPDHRPAGAPIEGSWLYVPNSNVRLMAFCSSHPAQVGPYHFGE
                     GSIDHDQCDLPSAASGWLEGQTLAFVIDFLDPQQKPVFRVFYQDAPTNPPIGHVPSAI
                     LGEKQVDLALLCVGSTDAVEDHPTQILGNLQPRYAISGHWEDFFQPVGSTPQPIPFLN
                     LDGYMQRAEAALPGRHTLAQPGARFMVPEGP"
     gene            941..2725
                     /locus_tag="VFV99_11315"
     CDS             941..2725
                     /locus_tag="VFV99_11315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059943.1"
                     /translation="MSSRDEDQATQLSSPRLPQQPPARVSRDSLHEVETSITPQNPRR
                     RMSTPSLGPKGSSLGGSNATTVGSPLEALEADEIMRTRLFCIIGMLIGAGGGASALVL
                     HGDPMITKLLLVAVIVALGGCLFLWLRTRDPIAFRRSSTQLGWFIPAACVTTSIPYYG
                     SFSPAAVVLVLGVYFTGLGKNSKLSYAVYAVCAGMQLLVGVLVIAGARDTGIINVERL
                     GLSTAEELILQGLVQMVFLATIVTARVSRRTALIAVGELERAVRIAAHREALLLEARE
                     ELERALRQGRGRFSDQTIGGYHLGQLLGRGAMGEVYEAEGAAGRVAVKLLSQASLSNP
                     NHVLRFLRELRTAANVRSPNIVQVIDVGEHPVPYLVMERLEGQTLAEIMRARGRPLGA
                     QEGVDLVAQIGNGITAAAIAGIVHRDLKPQNVFLDRGTWKILDFGVARAMDQGDTLTA
                     GQVVGTPSYMAPEQATGGAVDHRTDLYALAAIAYRALTGQPPYAAGEVAETLYKVVHT
                     KPRRPSELVDLPPEIDLVLAIGMAKNPEHRFASATELAAALAAAFAGALPEHVFERGR
                     VLERMGVWSSRAPTRASTSNMRAARPPR"
     gene            2797..2870
                     /locus_tag="VFV99_11320"
     tRNA            2797..2870
                     /locus_tag="VFV99_11320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2831..2833,aa:Pro,seq:tgg)
     gene            complement(2862..3209)
                     /locus_tag="VFV99_11325"
     CDS             complement(2862..3209)
                     /locus_tag="VFV99_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059944.1"
                     /translation="MRTWTRDQQNAAWRKWYRENAKRKVAWQRRRRKEMRGWWRDFKS
                     TKSCIKCGESAPECLHFHHRDPTEKEVDLSKIACSGQWSKKRILAEVAKCDVLCANCH
                     MKHHWEERLLSSG"
     gene            3788..5488
                     /locus_tag="VFV99_11330"
     CDS             3788..5488
                     /locus_tag="VFV99_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5059945.1"
                     /translation="MGGNAEQETAVLKMSAQTPSPRGTDERPAHAERTTIDPPPTTGS
                     STGSSLQALERDEILRSRAFSFLVMVLAIVGSIAAFVVPGGQPRGQLILVGGCALGFA
                     SMVFLLYQTLDPLRFRQPSTTLVWFLPAISVNAAVYYFGVFSPAVMLVVLGLYFVGLG
                     KSGRMAIAAYVFCALLLLGMALVPITGGHDIGIMVPPLSTTQQIMIHVLTQFILALTI
                     IIARLSRQSALVAVGELEQAVRLAAHRQALLLEARDELDRALRTKRGRFSEQTIGHYH
                     LGDVLGRGAMGEVYAAVDTRNSSEVAVKLLSQLSLGNANHVLRFFRELRTAASVESSH
                     VVRVIEIGEQPVPYLVMERLEGQTLAEILRERRTLPPNITLELVEQVGIGITAAGNAG
                     IVHRDLKPQNIFRHHDAWKILDFGVARAMDHGDTLTAGHLIGTPSYMAPEQASGGAVD
                     HRTDLYALAAVAYRALTGHPPFASGDIAETVYRVVHTRPIRPTLLAPELSGDVDLVFA
                     IGLAHDAADRFANAADLAAALRTAFARELSPALRARGHAIVDRAPWSTGRERPGKPSP
                     "
     gene            complement(5551..8523)
                     /locus_tag="VFV99_11335"
     CDS             complement(5551..8523)
                     /locus_tag="VFV99_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059946.1"
                     /translation="MQWLCAAVAVASAACTVDEVQNESVAAVTRLPASTNPYTLFESL
                     QVRPIALSPSGKFLFVANTPDNRLELFRVDHGNLVALGSVAVGLEPIAVAARSDNEVW
                     VVNHLSDSVSIVKVNNAGKASVAQTLLVGDEPRDIVFAGANKSRAFITTAHRGQNTGD
                     AYDLQTPGVGRADVWVFDANNLGSAPGGNRLTKLTLFADTPRALAVSGNGNTVYAAAF
                     LSGNQTTTVSEYAVQTMYPSGMPGPATIEVNGVTIPQPRTGLIVKYHNGHWVDAYGTV
                     FDPFVKIKLPDQDVFAIDATANPPRAIATGVYPHVGTTLFNMAVNPQSGKVYVTNTDA
                     HNDVRFEGHTPGFTSVRGHIADSRITVIDPAAGTVSPYDLNPHVDYTSEGTPGEKALS
                     VAAPQDVVVSADGSTLYTVAQGSSKLAIYRTVDLEAGNVAPSLTSQVALTGGGPTGVA
                     LDEANHRAYVLTRFDNSISIVDLAQRAEVGKVGLFNPEPESVVVGRKYLYDANLTSQH
                     GVTSCASCHIGGDKDELAWDLGNPGGAPLSITKLGDVFAISPQAIIAALPQTAPVFDF
                     NHPVKGPMTTQSLRGMDNHGAMHWRGDRNGAIQQDGTPFLDGNGQPVVSAQPNAGIYD
                     EVKAFTSFNVAFPGLIGRAAELSDSDMMEFATFILQASYPPNPIRNLDNSLTAEQQQG
                     RDFYFNHTIQNGVSVELPSDRFHNCNGCHTLDATANAGFTAHPGFFGTDGKLTFELET
                     QIFKVPHLRNLYTKIGMFGSSPDTLQPGTIILQGANVPVVDQVRGYGFQHDGSLGQLE
                     HFFTAQVFIKATTSVVLGGSTVAANPFGIPFVDLNALAQGQVVFVEGGGFALRHAIVA
                     FMLAFDSNFAPIVGQQITMTSANAATAGSRLDLLEARANAGECELVAKPTVGNGPNQG
                     MLYASGSWQRASTKVAGFSDATLRSKITSQEFPSVTFTCVPVGEGSRIAIDRDGDGYA
                     DWDEKTAGTDDADPNSHP"
BASE COUNT         1422 a         2945 c         2796 g         1552 t
ORIGIN      
        1 ggtgatgacc gcgccactgt tcacgcggca gaacgcgatc gaggtgacgt tcaacatcgc
       61 gctcgagccc gacaccgctg cgatcgacac tggcctcgcc gacgagccac tcgcaaacct
      121 gagcgcgatc gtgtcgggcc acgcgcacta cgatcacttc atggatgtgc cgcacatcct
      181 gacgaaggcg ccgaacgcga ccgcgtacac gaatctcacc ggtcgccacg tgcttgccgc
      241 gctcgcgccc gatcgcccga gctgcgcgaa tgcgacgccg tcgccggagc tgccgcgtga
      301 ccgcatcgtc gcgatggatg atccactcgc gagccacgtc gactacacga actgtccaga
      361 ccaccgccca gccggcgccc cgatcgaggg cagctggctc tacgtgccca acagcaacgt
      421 gcgcctcatg gcgttctgct cgtcgcatcc ggcgcaggtc ggcccgtatc acttcggcga
      481 aggctcgatc gatcacgatc aatgcgacct gccgagcgcc gcgtcgggct ggctcgaagg
      541 ccaaacgctc gcgttcgtga tcgacttcct cgacccacaa cagaagcctg tgttccgcgt
      601 gttctaccag gacgcgccga cgaacccgcc gatcggccac gtgccgagcg cgatcctcgg
      661 cgagaagcag gtcgatctcg cgctcctttg tgtcggcagc acggatgccg tcgaggatca
      721 cccgacccag atcctcggca atttgcagcc tcgctacgcc atctccggcc actgggagga
      781 cttcttccag ccggtcggct cgacgccaca gccgattccg ttcctcaacc tcgacggata
      841 catgcagcgc gccgaggccg ccctccccgg ccggcataca ttggcgcagc ccggagcgcg
      901 tttcatggtt cccgaagggc catgagcgta agctgaggag gtgagcagta gggacgagga
      961 ccaggcgacc cagctgtcgt cgccgcgtct accccaacag ccgcctgcgc gagtctcgcg
     1021 cgattccctg cacgaggtcg aaacgtcgat cacgccgcag aacccgcggc gccggatgtc
     1081 gacgccatcg ctcggcccca aggggtcgtc gctcggtggc agcaacgcga ccaccgtggg
     1141 ctcgccgctc gaagcgctcg aggccgacga gatcatgcgc acgcggctgt tctgcattat
     1201 cggcatgttg atcggcgcgg gtggcggagc ctctgcgctg gtgttgcacg gcgatccgat
     1261 gatcacgaag ctgttgctcg tcgccgtgat cgtcgcgctc ggcggttgcc tgttcttgtg
     1321 gctgcgcacg cgcgacccga tcgcgtttcg ccggtcatcg acgcagctcg gctggttcat
     1381 tcccgccgcc tgcgtgacca cctcgatccc gtattacggg tcgttttcgc ctgctgcggt
     1441 cgtgctcgtg ctcggcgttt acttcacggg tctcggcaag aactcgaagc tctcgtatgc
     1501 ggtctacgcg gtgtgcgcgg gcatgcagtt gctcgtcggc gttctcgtga tcgcgggtgc
     1561 acgcgacacc gggatcatca atgtcgagcg cctcgggctc tcgaccgccg aggagctcat
     1621 cctccagggg ctcgtccaga tggtgttcct cgcgacgatc gtcaccgcac gtgtgtcgcg
     1681 gcgcactgca ctgatcgccg tcggcgagct cgaacgcgct gtgcgcatcg ccgctcatcg
     1741 cgaagcgctg ctcctcgaag cccgcgaaga gctcgagcgc gcgctgcggc aaggtcgcgg
     1801 tcgcttcagc gatcagacga tcggcggcta tcacctaggc cagctgctcg ggcgcggcgc
     1861 gatgggcgaa gtctacgaag ccgagggcgc cgccggccgc gtcgccgtca agctgctctc
     1921 gcaggcgtcg ttgtcgaacc caaaccacgt gctccgcttc ctgcgcgagc tgcgcactgc
     1981 cgcgaacgtg cgctcgccga acatcgtgca ggtgatcgac gtcggcgagc acccggtgcc
     2041 gtacctcgtg atggagcggc tcgaaggcca gacgctcgcc gagatcatgc gcgcacgtgg
     2101 ccgcccgctc ggtgcgcagg aaggcgtcga tctcgtcgcg cagattggca atggcatcac
     2161 ggccgccgcg atcgccggca tcgtccaccg cgacctcaag ccgcagaacg tgttcctgga
     2221 tcgtggcacc tggaagatcc tcgatttcgg cgtcgcacgc gcgatggatc aaggcgacac
     2281 gctcaccgca ggtcaggtcg tcggcacgcc gtcgtacatg gcgcccgagc aagcgacggg
     2341 cggcgcggtc gatcaccgca ccgatctcta cgcgctcgcg gcgatcgcgt accgcgcgct
     2401 gaccggccag ccgccctacg ccgccggcga ggtcgccgag acgctctaca aggtcgtgca
     2461 cacgaagccg cgtcggccga gcgagctcgt cgacctcccg cccgagatcg atctcgtgct
     2521 cgcgatcggc atggcgaaga atcccgagca ccgcttcgcg agtgcgaccg agctcgccgc
     2581 cgcactcgca gccgcgttcg caggagccct ccccgagcac gtcttcgagc gtggccgcgt
     2641 gctcgaacga atgggcgtct ggtcgtcgcg ggcaccgacg cgggcgtcga cgagtaatat
     2701 gcgcgcagca cggccgccgc gctgattcgc acagcgacgg gcgtttatcg cttcaggact
     2761 tgcggagcgt gcagttacca ggtatccgaa gcggtacggg gtgtagcgca gcctggtagc
     2821 gcaccgcatt tgggttgcgg gagccgctgg ttcgaatcca gtcaccccga cgacaacaag
     2881 cgttcttccc agtggtgctt catgtggcag ttcgcgcaga gcacatcgca cttcgcgact
     2941 tcggcgagaa tcctcttctt tgaccactgc cccgaacagg caatcttgct gaggtcgacc
     3001 tccttctcgg tcgggtcacg atgatgaaag tgcaggcact cgggtgcgct ctcgccgcac
     3061 ttgatgcacg acttcgttga cttgaagtcg cgccaccagc cgcgcatctc cttgcgtcga
     3121 cgccgttgcc acgccacctt ccgcttagcg ttctcgcgat accacttgcg ccacgccgcg
     3181 ttctgttgat cgcgcgtcca cgtcctcacg acgcacgctc ctttgcgtga tgctttgcat
     3241 ggcagttcgc gcatagcacc tcgcacttcg caagctcagc gagaattcgc tcgcgactcc
     3301 aaccgcggcg aactgcatcc gaaacggata cctccttcgc cgtcggatcg gagtgatgga
     3361 attgcaggca tccagggtgt gactcgccgc aacgcgcgca gaccagcttg ctcttgagct
     3421 ccgcgaacca ctcgacgagc gagtgtcgcc gcgccatctt cgtctgccgc tcgcgctcga
     3481 cgagctcagg agttcttcgc tcgtgcttcg tacgctggta ccaagcgcgc accgtcgcac
     3541 gccgcttctc tggatccttt gccggcatga cagcgcacgc tgatcacgcc acgactgtcc
     3601 ggcaactctc ggacagctga acgacgaagc gccagccctc ccacgcgctg gcgctctgag
     3661 tgttcgtgtc cgaaccgcgg actaattggc gccccgtcgt gagctcaaca tggtcaactc
     3721 gcgcaccact gctctcgcca cttctacgtg cgatccgcgg gagcgcggtg cgatatgcta
     3781 gaggctcatg ggggggaacg cagagcaaga gacggcggta ttgaagatgt cagcgcagac
     3841 gccgagtccg cgcggcaccg acgaacggcc cgcccatgca gagcgcacga ccatagaccc
     3901 gccgccaacg accgggtcga gcactggctc gtcgctgcag gcgctcgagc gcgatgagat
     3961 cctacggtcg cgtgcgttta gcttcctcgt gatggtgcta gcgatcgtcg gttcgattgc
     4021 cgcgttcgtg gttcccggcg gtcagccgcg cggccagctg atcttggtcg gagggtgcgc
     4081 gctcgggttc gcatcgatgg tgttcttgct gtaccagacg ctcgatccgc tgcgctttcg
     4141 ccagcccagc acgacgctcg tctggtttct acccgcgatc tccgtaaacg ccgccgtcta
     4201 ctacttcggc gtgttctcgc cggcggtgat gctggtcgtg ctcggcctgt acttcgtcgg
     4261 actcggcaag tcgggcagga tggcgatcgc ggcgtacgta ttctgcgctc ttctgctgct
     4321 cgggatggca cttgtgccca tcaccggcgg acacgacatc ggcatcatgg tccctccgct
     4381 gagcacgaca cagcagatca tgatccatgt gctcacgcag ttcatcctcg cgctgacgat
     4441 catcatcgcg cgcctgtcgc gccagagcgc gttggtcgcg gtcggcgagc tggaacaagc
     4501 ggtacggctc gcggcgcacc gccaagcgct gttactcgag gcacgtgacg agctcgaccg
     4561 agcgctacgt acgaagcgcg gccggttttc cgagcaaacc atcggccact accaccttgg
     4621 cgacgtactt ggtcgcggcg cgatgggcga ggtctatgcc gccgtcgaca cgcggaacag
     4681 ctcggaggtc gcggtcaagc tgctgtccca actctcgctc ggcaacgcga accatgtgct
     4741 gcgcttcttt cgcgagctgc gcaccgccgc gagcgtggaa tcgtcccacg ttgtccgcgt
     4801 gatcgagatc ggcgaacagc ccgtgccgta cctggtcatg gaacgattgg agggtcagac
     4861 gctcgcggag atcctgcgcg agcgccgcac gctaccgccg aacatcacgc tcgagctcgt
     4921 cgagcaggtc ggcatcggca tcaccgcagc cggcaacgcc ggcatcgtgc atcgcgatct
     4981 caagccacaa aatatctttc gccaccacga cgcgtggaag attctcgatt tcggcgtcgc
     5041 gcgcgcaatg gaccacggcg acacgctgac cgcgggccac ctcatcggta cgccgtccta
     5101 tatggcgccc gagcaggcaa gcggcggcgc cgtcgatcac cgcaccgatc tctacgcgct
     5161 agcagcggtc gcgtatcgcg cgctgaccgg gcatccaccg ttcgcgtctg gcgacatcgc
     5221 agagaccgtc taccgcgtcg tacacacgcg gccgatacgc ccgactctgc tcgcaccgga
     5281 gctgtccggc gacgtcgatc tggtcttcgc gatcgggttg gcgcatgacg cggccgaccg
     5341 gtttgcgaac gccgccgacc ttgcggccgc gctacgcacc gcgttcgcgc gcgagctatc
     5401 gccagcgctg cgcgcgcgtg gtcacgcgat cgtcgatcgg gcgccctggt caaccggacg
     5461 cgagcgtccg ggtaagccgt cgccgtgacc aaaacgacga agcgccggct cttccgaacc
     5521 ggcgcttcgc tgcgactccg aggtactcgg ctacgggtgg ctgttcggat cagcgtcgtc
     5581 cgtgcccgcc gtcttctcgt cccagtccgc gtagccatcg ccgtcgcgat cgatcgcgat
     5641 gcgcgaccct tcgccgaccg gcacgcaggt gaacgtcacc gacgggaatt cctgcgacgt
     5701 gatcttcgaa cgcagcgtcg catccgagaa tcccgccacc ttcgtcgacg cccgttgcca
     5761 gctaccgctc gcgtagagca tgccctggtt cggaccgttg cccacggtcg gcttcgcgac
     5821 gagctcgcac tcacccgcgt tggcacgcgc ttcgagtagg tcgagacgcg agccggcggt
     5881 cgcggcgttc gccgaggtca tcgtgatctg ctgaccgacg atcggcgcga agttcgagtc
     5941 gaacgcgagc atgaacgcga cgatcgcgtg acgcagcgcg aagccaccgc cctcgacgaa
     6001 cacgacctgg ccttgcgcga gcgcgttgag gtcgacaaat ggaatgccga acggattcgc
     6061 tgcgaccgtc gagccaccga gcaccaccga cgtcgtcgcc ttgatgaaga cctgggccgt
     6121 gaagaagtgc tcgagctgac cgagcgagcc gtcgtgctgg aagccgtagc cgcgcacctg
     6181 gtcgacgacc ggcacgttcg cgccctggag aatgatcgtg cccggctgca gcgtatccgg
     6241 cgagctaccg aacatgccga tcttcgtgta caggttccgc aggtgcggga ccttgaagat
     6301 ctgggtctcg agctcgaacg tcagcttgcc gtcggtgccg aagaaaccgg gatgcgccgt
     6361 gaagccggcg ttcgcggtcg cgtcgagcgt gtggcaaccg ttgcagttgt ggaagcgatc
     6421 cgacggtagc tcgaccgaga cgccgttctg gatcgtgtgg ttgaagtaga agtcgcgacc
     6481 ctgctgctgc tcggccgtca gcgagttatc gaggttgcgg atcgggttcg gcggatacga
     6541 cgcctgcagg atgaacgtcg cgaactccat catgtcggag tcggagagct ccgcggcgcg
     6601 gccaatcaaa cctgggaacg cgacgttgaa cgacgtgaac gctttgacct cgtcgtagat
     6661 gccggcgttc ggctgcgccg acacgaccgg ttggccgttg ccgtcgagga acggcgtgcc
     6721 gtcctgctga atggcaccgt tgcggtcgcc gcgccagtgc atcgcgccgt ggttgtccat
     6781 gccgcgcaag ctctgggtcg tcatcgggcc tttgaccgga tgattgaagt cgaacaccgg
     6841 cgcggtctgc ggcagcgcgg cgatgatcgc ctgcggcgag atcgcgaaca cgtcgccgag
     6901 cttcgtgatc gacagcggcg caccacccgg gttgccgagg tcccatgcga gctcgtcctt
     6961 gtcgccaccg atgtggcaac tcgcgcacga ggtcacgccg tgctgcgagg tgagattcgc
     7021 gtcgtacagg tacttgcgac cgacgaccac gctctccggc tccgggttga acagtccgac
     7081 tttgccgacc tcggcgcgct gcgcgagatc gacgatcgag atgctgttgt cgaagcgcgt
     7141 gagcacgtac gcgcggtggt tcgcttcgtc gagcgcgacg cccgtcggac caccgccggt
     7201 gagcgcgacc tgactggtca ggctcggcgc gacgttaccg gcctcgagat ccaccgtgcg
     7261 gtagatcgcg agcttcgacg agccttgcgc gaccgtgtac agcgtcgagc cgtccgcgga
     7321 gacgacgaca tcttgcggcg cggcaacgct gagcgccttc tcgcccggcg tgccttcgct
     7381 cgtgtagtcg acgtgcgggt tgagatcgta gggcgacacc gtgcccgccg cgggatcgat
     7441 caccgtgatg cggctgtcgg cgatgtgacc acgcaccgag gtgaagcccg gcgtgtggcc
     7501 ctcgaagcgc acgtcattgt gcgcgtcggt gttcgtgacg tagaccttgc cgctctgcgg
     7561 gttgaccgcc atgttgaaca gcgtcgtgcc gacgtgcggg tagacgccgg tcgcgatcgc
     7621 gcgcggcgga ttcgcggtcg catcgatcgc gaacacgtct tgatcgggaa gcttgatctt
     7681 gacgaatgga tcgaacaccg tcccgtacgc gtcgacccag tggccgttgt gatacttcac
     7741 gatgagaccg gtgcgcggct gtggaatcgt cacgccgttg acctcgatcg tcgccggacc
     7801 gggcatgccg ctcgggtaca tcgtctgcac cgcgtactcc gacaccgtcg tggtctggtt
     7861 gcccgacaga aatgcggccg cgtagaccgt gttgccgttg ccggacaccg cgagtgcgcg
     7921 cggcgtgtcg gcgaacagcg tcagcttcgt caggcgattg ccaccgggcg cgctgccgag
     7981 gttgttcgcg tcgaataccc acacatcggc gcgaccgacg ccgggcgtct gtagatcgta
     8041 cgcgtcgccg gtgttctggc cgcgatgtgc cgtcgtgatg aacgcgcggc tcttgttcgc
     8101 accggcgaac acgatgtcgc gcggctcgtc tccgacgagc agcgtctgcg ccaccgacgc
     8161 cttaccggcg ttgttgacct tgacgatgct gaccgagtcc gagaggtggt tgacgaccca
     8221 cacttcgttg tcgctgcgcg cggccaccgc gatcggctcg agaccgaccg cgaccgagcc
     8281 gagtgcgacg agattcccgt gatcgacgcg gaacaactcg agccggttgt cgggcgtgtt
     8341 cgcgacgaac aggaacttgc ccgacggcga tagcgcgatc gggcgaacct gaaggctctc
     8401 gaacaacgtg tacggattcg tgctggccgg taatcgcgtg accgcggcga ccgactcgtt
     8461 ctgcacttca tcgacggtgc acgcggcgga cgccaccgcc accgctgcac atagccactg
     8521 catcttcatc atagggcgcg agcgttacgt aagacgcgcg ctcagatgaa gcagtaaggt
     8581 ttgttgggcc gcgctcgtta atccgcgtca acagagcaat gttggcgcga gcgcgattct
     8641 cgtcgagcaa tcattgctcg gatccgaaaa aacgaagcgc cggctccccc acggcgccgg
     8701 cgctttgacg gttcg
//
LOCUS       DASUFX010000285         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_183387,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000285 DASUFX010000000
VERSION     DASUFX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183387"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFV99_11340"
     CDS             complement(<1..1076)
                     /locus_tag="VFV99_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059947.1"
                     /translation="MRPGADDQQPPDEVSPTAVYLTPPQHLTRISLALRGMRPSAADL
                     EAVEADPDVLPSIVDRYLATPAFAETIKELHNETLLVRIEVPQFTYPAIGPLAGVTAR
                     DINTGKFDEPLRLIEDIVMNDQPYTKIVTADYTMTNSVTAAMWGMTHSGAPDAWTRTP
                     LPDARPKLGILSSNALYDRWRSAGFNYNRGRANLISRALLCHDFLSSDIVVDTSIDLA
                     DPDVVANAVMKNSSCAGCHQTLDPLASYLFTFRNTLAPVGVSAYPVTYYSPGTANRWM
                     TANKRPPMFFGQSTGDTTGLGTAIANDPRFAKCTAMRFASYFTEVAQNELSNAWIARL
                     QKALVDSNFNAKALTKAIMLSDEFR"
     gene            1279..2583
                     /locus_tag="VFV99_11345"
     CDS             1279..2583
                     /locus_tag="VFV99_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5059948.1"
                     /translation="MSEIETQLQQRPGVAVVSTLRVEVIDGPDRGANASSNDALSVGT
                     AKDNALVIGDFTVSRYHLEASPHPGGVLVVDLGSTNGTYVGAVRVERAIVPPGTPITL
                     GGTTIRFVDAVKRTLTTAGGVQQHAGMIARSPQLLKLFADIERIAATQTSVLIVGESG
                     TGKERVAEALHARSGRSGQPLVTIDCGALTSALLASELFGHERGAFTGADRVHVGAFE
                     RARGGTVFLDEIGELPAADQSSLLGVLERKRFRRVGGSQEIELEARVIAATNRDLRAE
                     VNNGRFRHDLYHRLAVVVLRLPPLRERREDILPLVEHFARELGANDTLENVFGAETLA
                     RWQRHPWPGNVRELRNAVEAAIVVGPHAYCDQALQTASVATDGPLIPYKDARSAVVRE
                     FERDYLVRLLADANGNVSQAARTAKMDRSHLIDLLHRHGLKE"
     gene            complement(2573..4078)
                     /locus_tag="VFV99_11350"
     CDS             complement(2573..4078)
                     /locus_tag="VFV99_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059949.1"
                     /translation="MAMVKLALLVAIALASGCFDDRYHCTSDMQCNLGEGGRCEVDGN
                     CTVHDTRCPTLRRYAAHSGELTDQCFDDSVAPVNPCAGGQPPAKAEGCFADVCDRLPA
                     CCAVGWFDACAQVAQETASCNLRCDTRIAITATRSTTTEQWDARWNGDQWSFTARDDL
                     AALQWVAPAPGDSEPRLCGATAEELVIGDTHIPVPPGRSYGSITSIGFDRDRRDTIAA
                     TYNDGRNRLELWKLDKLTVHEQGVPGTGGLTWGEADRDSYPDAIVKVGGSQFGFLYNV
                     EDDTFQHSLLNQTAGSMAGGATPGAPQVRSFDWLDINDDNKLDLAIFGAEVRMHTNQL
                     GLGDPAARQFDCDPPTAARECSMDTNEPNLEASSFAGAGLPVAGDASIVFSLFPGRQV
                     YRAKPDGSVAPFKFPGDTCSCVKTCTGGCPGPSCTCTYNCNACVPVLAFVARDVDGDH
                     ALDLIAIDAKLQIFVAKAANGYQWGGATSIPTAFPNTFFSVDVSVTGAPIP"
     gene            complement(4066..>4375)
                     /locus_tag="VFV99_11355"
     CDS             complement(4066..>4375)
                     /locus_tag="VFV99_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059950.1"
                     /translation="RLAQHQEQEKQQPPPPPPPERPAVEPRRWHAEDSGLVALGGAAM
                     LVGAGLLFYSHRLGDDHGGTLADYDERVRQSRTTMWTGAGVATAGALVIGVTVLRWRW
                     "
BASE COUNT          736 a         1446 c         1463 g          730 t
ORIGIN      
        1 cggaactcgt cggacaacat gatcgccttc gtcagcgcct tcgcgttgaa gttgctgtcg
       61 acgagcgcct tctgcaggcg cgcgatccac gcgttcgaca gctcgttctg cgcgacctcg
      121 gtgaagtacg acgcgaagcg catcgcggtg cacttcgcaa agcgcgggtc gttcgcgatc
      181 gcggtgccga ggccggtcgt gtcaccggtc gattgaccga agaacatcgg cggccgcttg
      241 ttcgcggtca tccagcgatt cgcggtgccc ggcgagtagt acgtgacggg gtacgcgctg
      301 accccgaccg gcgcgagcgt gttgcggaac gtgaacagat agctcgcgag cggatcgagc
      361 gtctgatggc aacccgcaca cgacgagttc ttcatcaccg cgttcgcgac gacatcgggg
      421 tcggctagat cgatcgacgt atcgacgacg atgtcgctcg agaggaaatc gtggcacaac
      481 agcgcgcgcg agatcagatt cgcgcggccg cggttgtagt tgaagccggc ggaccgccag
      541 cgatcgtaga gcgcgttcga tgacagaatg ccgagcttcg ggcgcgcgtc gggcagcgga
      601 gtgcgcgtcc aggcgtccgg cgcgccggaa tgggtcatgc cccacatggc cgcggtcacg
      661 ctgttggtca tcgtgtagtc ggccgtgacg atcttcgtgt acggctgatc gttcatcacg
      721 atgtcttcga tgaggcgcag tggctcgtcg aatttgccgg tgttgatgtc gcgcgccgtg
      781 acgccagcga gcgggccgat cgcggggtac gtgaactgcg gtacctcgat gcgcacgagc
      841 aacgtctcgt tgtgcagctc cttgatcgtc tcggcaaatg ccggcgtcgc gaggtagcga
      901 tcgacgatcg atggcaacac gtccggatcc gcctcgaccg cttcgaggtc ggcagcggac
      961 ggccgcatcc cgcgcagcgc gagcgagatc cgggtcagat gctgcggcgg cgtcagatac
     1021 accgccgtcg gactgacctc atccggtggc tgctgatcgt cagcgccggg acgcacgtca
     1081 ctggcgcagg cagttaggca cactgtgatt gcgaggagcc gcatggttgc cgtacctaca
     1141 gcaaggtgag gaccaggcgt cgaccagctc ggaccgcgtt atcccgtgcg cttatgtggt
     1201 taactaaaca gccattgtcg ggtcagacct gcacgttgta ggggacaccc gacaaaccga
     1261 cggtgtaagc gcggtgaaat gagcgagatc gaaacccagc tccagcagcg ccccggcgtg
     1321 gcggtcgtca gcaccttgcg cgtcgaggtc atcgacggcc cggatcgcgg cgcgaacgca
     1381 tcgtcgaatg acgcgttgtc ggtcggcact gcaaaggaca acgcgctcgt catcggcgac
     1441 ttcaccgtgt cgcgctatca cctcgaagca tcgccacatc cgggtggcgt gctcgtggtc
     1501 gatctcggct cgacgaacgg cacgtacgtc ggcgccgtgc gggtcgagcg cgcgatcgtt
     1561 cctcccggta cgccgatcac gctcggcggc accacgatcc gcttcgtcga cgcggtcaag
     1621 cgcacgctca ccaccgccgg cggggtccag caacacgcag gcatgatcgc gcgctcgccg
     1681 cagctgttga agctgttcgc cgacatcgag cgcattgcag caacgcagac gtcggtgctg
     1741 atcgtcggcg aatcgggcac cggcaaggag cgcgtcgccg aggcgttaca cgcgcgctct
     1801 ggacgcagcg gtcagccact cgtcacgatc gactgcggtg cattgacgag cgcgttgctc
     1861 gcgagcgagc tgttcggtca cgagcgcggt gcattcaccg gtgcggatcg cgtgcacgtc
     1921 ggcgcgttcg agcgcgcgcg gggaggcacc gtgtttctcg acgagatcgg ggagctgcct
     1981 gccgcggatc aatcgtcgct actcggcgtg ctcgagcgca agcggttccg ccgcgtcggc
     2041 ggctcgcaag agatcgagct cgaagcacgt gtgatcgcgg cgacgaatcg cgacctgcgg
     2101 gcggaggtca acaacggccg cttccggcac gacctctatc accggctcgc ggttgtcgtg
     2161 ctgcgcttgc cgccgctgcg cgagcgccgc gaggacatct tgccgctcgt cgagcacttc
     2221 gcacgcgagc tcggcgcgaa cgacacgctc gagaacgtgt tcggcgccga gacgctcgcg
     2281 cgctggcaac ggcatccgtg gccgggcaac gtgcgcgagc tgcgtaacgc ggtcgaggcc
     2341 gcgatcgtcg tcggtcccca cgcgtattgc gatcaggcgc tgcaaaccgc gagcgtcgcc
     2401 accgatgggc cgctgatccc gtacaaggac gcgcgcagcg cggtcgtgcg cgagttcgaa
     2461 cgcgattacc tcgtgcggtt actcgccgac gcgaacggca acgtgtcgca ggctgcgcgc
     2521 acggcgaaga tggatcgctc gcacctgatc gatctcttgc accgtcacgg cctcaaggaa
     2581 taggcgcgcc ggtcaccgac acgtcgacgg agaagaacgt gttcgggaac gcggtcggga
     2641 tcgacgtcgc gccgccccac tggtaaccat tcgccgcctt cgcgacgaag atctggagct
     2701 tcgcgtcgat cgcgatcaga tcgagcgcgt gatcgccgtc gacgtcgcgg gcaacaaacg
     2761 cgagcaccgg cacgcacgcg ttgcagttgt acgtgcacgt gcagctcgga cctggacagc
     2821 caccggtgca ggtcttcacg cacgagcagg tatcgccggg aaacttgaac ggggcgacgc
     2881 tgccgtccgg cttcgcgcga tagacctggc gaccgggaaa cagcgagaac acgatcgacg
     2941 catcgccggc gaccggtaag ccagcgccgg cgaacgacga cgcctcgaga ttcggctcgt
     3001 tcgtatccat cgaacactcg cgcgccgcgg tcggcggatc gcaatcgaac tggcgcgccg
     3061 ctggatcgcc gaggccgagc tggttcgtgt gcatgcgcac ctcggcaccg aagatcgcga
     3121 gatcgagctt gttgtcgtcg ttgatgtcga gccagtcgaa cgagcgcact tgcggcgcgc
     3181 ctggcgtggc accgcccgcc atcgagccgg cagtctgatt gagcaagctg tgctgaaacg
     3241 tgtcgtcctc gacgttgtaa aggaaaccga actgcgagcc gccgaccttc acgatggcat
     3301 cgggatacga gtcgcgatcc gcttcacccc acgtcagccc accggtgcct ggcacgcctt
     3361 gctcgtgcac ggtcagcttg tcgagcttcc acagctcgag ccgattacgg ccatcgttgt
     3421 acgtcgcagc gatcgtgtcg cgccgatcac gatcgaagcc gatcgacgtg atcgacccgt
     3481 agctgcggcc cggcggcacc ggaatgtgcg tatcgccgat caccagctcc tccgccgtcg
     3541 ctccacatag ccgaggctcg ctgtcacctg gcgccggtgc gacccactgc agtgccgcga
     3601 gatcatcgcg cgcggtgaag ctccactggt cgccattcca tcgcgcatcc cattgctcgg
     3661 tcgtggtgct gcgcgttgcg gtgatcgcga tccgcgtatc acagcgcagg ttacacgacg
     3721 cagtctcctg cgcgacctgc gcgcatgcgt cgaaccaacc gaccgcgcag caagccggca
     3781 gccgatcgca cacgtctgcg aagcagccct cggccttcgc cggtggctga ccgcccgcgc
     3841 acggattgac cggggcgacg ctgtcgtcga agcactggtc cgtgagctca ccactatgcg
     3901 cggcgtaacg gcgcagcgtc gggcagcgcg tgtcgtgcac cgtgcagttg ccatcgacct
     3961 cgcagcggcc gccctcgccg aggttgcact gcatgtcact cgtgcagtga tagcgatcgt
     4021 cgaagcagcc gctcgcgagc gcgatggcga ccaggagcgc gagtttcacc atcgccacct
     4081 gagcacggtc acgccgatca cgagcgcgcc cgccgtcgcg acaccggcgc cggtccacat
     4141 cgtcgtgcgt gattgccgaa cccgttcgtc gtaatcggcg agcgtaccgc catggtcgtc
     4201 gccgagccgg tggctgtaga acaacagccc cgcgccgacg agcatcgcgg caccaccgag
     4261 cgcgacgagc ccgctgtcct cggcgtgcca ccggcgcggc tcgaccgctg gccgctcggg
     4321 cggtggtggt ggtggcggtt gctgcttctc ttgctcctga tgctgcgcga gtcgc
//
LOCUS       DASUFX010000286         6180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1294493, whole genome shotgun sequence.
ACCESSION   DASUFX010000286 DASUFX010000000
VERSION     DASUFX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294493"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFV99_11360"
     CDS             complement(<1..344)
                     /locus_tag="VFV99_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5059951.1"
                     /translation="MKCAVSIDLDGVACYYRIHGLGPAPAELEHVILERALPRAAALF
                     AHKKMRVTWFVIGRDADADAALPGHTARVANAQRLRTLFDLGDELGNHSYSHPYDLAR
                     LSPSAVDSEIAN"
     gene            complement(341..1075)
                     /locus_tag="VFV99_11365"
     CDS             complement(341..1075)
                     /locus_tag="VFV99_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5059952.1"
                     /translation="MAPDVSIVIPVYNEEGILREAITELISGLDSVRLALDDQDMTFE
                     IIIAENGSRDSTVELAEHLATEMPEVRTFSLGEPNYGKALRRGILEARGTWVICEEID
                     LCDLDFHRRALELLRHGDADMVVGSKAMKGASDHRPLFRRAATRVLNGMLRVALDFRG
                     TDTHGLKAFHRETLLATVEECVIDRDLFASEFVIRAGRAGMRVIEIPIRLDEKRPPAI
                     NLVKRVPNVLRGMAKLTYVIRFGGKP"
     gene            complement(1088..2002)
                     /locus_tag="VFV99_11370"
     CDS             complement(1088..2002)
                     /locus_tag="VFV99_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5059953.1"
                     /translation="MSTARAGELAKLHRNIPVQRYRSLVGHWITRWFRPPRPAHHEAI
                     VNPEPGQCVVTFGGHATVLARYPQLSIAFDPMLGRWVGGVRRAVEPGLAPGDFEDVGL
                     VLISHRHADHLHLATLRKLPRKSATIVVPTGGAAWVSSLGFARVIELAPGADLELRGI
                     QVIAYATAHGDSDLARGLSYVVRPSPETEAVGPSLYFCGDSGYFSGFADIGERFAPDI
                     AMLPIGGFYPSSFRSRHMSPLDALYAFEDLRARLLVPIHHGAFALSYERLDEPVRWLL
                     ELASQRGIREHVMVMKAGQTERFNASRA"
     gene            2036..4405
                     /locus_tag="VFV99_11375"
     CDS             2036..4405
                     /locus_tag="VFV99_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5059954.1"
                     /translation="MEPLSRYLGRWPIALACVVVLAAAVLLPGLGTFGIWEPQERQLA
                     DRVAPRAELAKQQASAMPQTPPPAPKDGCWRIAPPDSVARTLTNKAMVFGRDTFGDSD
                     AGRRLPLALLGLIAVLAAAGIAMRSSGPRAGIVTALVLLSMPLLVFQSRQLTSEIGTA
                     TGATLIIYGLLALGRPAQRWWWIDLPVSLFALAAGCALAFMSGGALLGLLVPLAAFAV
                     AGKLGVPFAIDVGRAARNGALRVAKRVAPRFAVGREPTAYVTGDNWPAFVATLFTIAV
                     IGVLAYQIFSLVEPQPGMTPPQRALFGKAVVTTGCWSSALGGIWRPDDDLRYVFDSTF
                     EQIAYGTFPWGILGIVAMAALLASDEPRKRLIGALTLAWAGGAWITAEVFQRKVGFTI
                     WAGFPALAIAVGVWLDSALASRAMVPAKDRSALPKAAILIGLFVALAIVNLGKDMQSF
                     SERISSLLIGSDQIAYPKMSKLLFLPTRVWILVVGALVATGFVLAMLDFRRVWRQGIA
                     IALGGTVLAAAFWSFGWLPALARNLSSKAMFDTYLELRKPGDQLVIMGDMGDAPHDYA
                     PDAKVEQLTSRDQIVQAIGRPNRVFAIAPQTELCQLHREIGGKDYFVLDDRNTRSLLL
                     SNKVTGTTDKNPLRTAILHSEPINIPIRPKGKIVFDGRIELLGWSMPKRVGRGDKFEV
                     MMVYKVLQPVPGNWRVLFHFDGALRFNGDHEPIKGRCQTSTWQPGDFIVDRYTVTAGA
                     GAFTPGPYDVWTGFFTGSNPNWKNMTVSEAPADMRDQADRVKITTITLD"
     gene            4453..4839
                     /locus_tag="VFV99_11380"
     CDS             4453..4839
                     /locus_tag="VFV99_11380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEX5059955.1"
                     /translation="MVRSKGVTAEVPTADHDRRQAPRVLVDLEVDYASEDNYLFAYIT
                     DISETGIFVRTTTPEQPGTHLNLRFRPDDEGPQIEVEGLVIWINPFRPGAPDNLHPGM
                     GIRFVALDDELRDRLLELVRRFAYLS"
     gene            4846..6066
                     /locus_tag="VFV99_11385"
     CDS             4846..6066
                     /locus_tag="VFV99_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5059956.1"
                     /translation="MSIICGTDLTAAGAGALEVARALAAQRGDREVILVHVAEDEAQA
                     ESARAPLDAQAATPGIPVRAELVVGQTDERLVSFAETEGSDLIVIAASAGSRKLGATA
                     ERVIVAARTPVLVVRDPAPWLAFAKGAKPLRVLLGVDDSVACDLGIQWTHALRQRAPV
                     EVVLGAIYYPDDAAEYYGLHAGAMVDRDPEIEKLVVRDLMNKFGGGSLVQAIAKRGLG
                     RIGDHVVELGTETNVDAIIVGTSQKTGLGRLGSVSQVIVHDAPQSVVCVPPNANVPTI
                     SVPRLTRALVATDLSAFANRAVPFAFALTDEGGEVHIAHVVDEDAEIDENDAQRQLLA
                     LAPASMKRQVAAHVVRGSDAARAIAQAAARLGCDAICIASHGRSGITRALVGSVADKL
                     LRATRKPVLVLRPA"
BASE COUNT          991 a         2026 c         2099 g         1064 t
ORIGIN      
        1 cagttcgcga tctcgctatc gactgccgac ggcgacagcc gcgcgagatc gtacggatgc
       61 gagtacgagt gattgccgag ctcgtcgcct agatcgaaca gcgtgcgcag ccgctgcgca
      121 ttcgcgacac gcgcggtgtg ccccggcaat gccgcgtcgg cgtcggcatc gcgaccgatc
      181 acgaaccacg tcacgcgcat cttcttgtgc gcgaacaggg cggctgcacg cggcagcgcg
      241 cgctcgagga tcacgtgctc gagctcggcg ggtgccggcc cgaggccatg aatgcggtag
      301 taacaagcga cgccatctag atcgatcgag acggcacact tcatggcttg ccgccgaatc
      361 ggatcacgta ggtgagcttc gccatgccgc ggagcacgtt cgggacgcgc ttgacgaggt
      421 tgatcgccgg cgggcgcttc tcgtcgagac ggatcggaat ctcgatcacg cgcatgccgg
      481 cgcggccggc gcggatcaca aactcgctcg cgaacagatc gcgatcgatc acacactctt
      541 cgacggtcgc cagcaacgtc tcgcgatgaa atgccttgag gccgtgcgta tccgtaccgc
      601 ggaagtcgag cgcgacgcgc agcatgccgt tgagcactcg cgttgcggcg cgccggaaca
      661 gcggccgatg atcgctcgcg cccttcatcg ccttgctgcc aacgaccatg tcggcgtcgc
      721 cgtggcggag tagctcgagt gcgcggcgat gaaagtcgag gtcgcagaga tcgatctctt
      781 cgcagatcac ccacgtgccg cgcgcctcga ggatgccgcg gcgcagcgcc ttgccgtagt
      841 tcggctcgcc gagcgaaaac gtgcgcactt ctggcatctc ggtcgcgagg tgctcggcca
      901 gctcgaccgt gctgtcacgc gagccgttct cggcgatgat gatctcgaac gtcatgtcct
      961 gatcgtcgag cgcgagccgc accgaatcga ggccggagat cagctcggtg atcgcctcac
     1021 gcaggatccc ttcctcgttg tagacgggga tcacgatcga gacgtcagga gccaccgcgg
     1081 gtttttatca cgctcgcgat gcgttgaaac gctcggtctg gcccgccttc atgaccatca
     1141 cgtgctcgcg aatgccgcgc tggctcgcga gctcgaggag ccagcgtacg ggctcgtcga
     1201 ggcgctcgta cgacaacgcg aacgcgccgt ggtgaatcgg aaccagtagt cgcgcgcgca
     1261 gatcttcgaa cgcgtagagc gcgtcgagcg gcgacatgtg acggctgcga aacgagctcg
     1321 gatagaagcc gccgatcggc agcatcgcga tgtcgggtgc aaagcgttcg ccgatgtcgg
     1381 cgaagcccga gaagtacccc gagtcaccgc agaagtagag gctcggtccc acggcttcgg
     1441 tctccggaga tggcctgacg acgtaggaca atccccgtgc gagatcgctg tcgccgtgcg
     1501 cggtcgcgta cgcgatcact tgtatgccgc gcaactcgag gtcggcgccg ggcgcgagct
     1561 cgatgacgcg cgcgaagcca agcgacgaca cccacgccgc gccgccggtg ggcacgacga
     1621 tcgtcgcgct cttgcgtggc agcttgcgca gcgtcgccag gtgcagatgg tcggcgtggc
     1681 ggtgcgagat cagaacgagc ccgacgtcct cgaagtcgcc aggcgcgaga cctggttcga
     1741 ccgctcgccg cacgccgccg acccagcgcc cgagcatcgg gtcgaacgcg atcgatagct
     1801 gcgggtagcg cgcgagcacg gtcgcgtggc cgccaaaggt cacgacgcac tgccctggct
     1861 cggggttcac gatggcttcg tggtgggccg ggcgcggcgg ccggaaccac cgtgtgatcc
     1921 aatgcccaac gaggctgcgg taccgctgca ccggtatgtt gcgatggagc tttgcgagct
     1981 cgccggcgcg tgcagtcgac acgaagaacg atagtaacag tggtactttc gcgccgtgga
     2041 gcccctgtcc cggtatctcg gtcgctggcc gatcgcgctc gcatgcgtgg tcgtgcttgc
     2101 ggcggccgtg ctgctgccgg ggctaggcac gtttggcatc tgggaaccgc aggaacgtca
     2161 gctcgccgat cgcgtcgcgc cgcgcgccga gctcgcaaag cagcaagcgt ccgcgatgcc
     2221 gcagacgcca ccaccggcgc cgaaagacgg ctgctggcga atcgcaccac cggattcggt
     2281 cgcgcgcacg ctgacgaaca aagcgatggt gttcggacgc gacacgttcg gcgacagcga
     2341 cgcgggtcgg cgcttgccgc tcgcactgct gggcctgatc gccgtgctcg ctgccgcagg
     2401 tatcgcgatg cgctcgtcgg ggccgcgtgc cggcatcgtc accgcgctcg tgctgctgtc
     2461 gatgccgctg ctcgtgttcc agtcgcgcca gctgacgagc gagatcggga ccgcgaccgg
     2521 cgccacgctc attatctatg gcttgctcgc gctcggccga cccgctcagc gctggtggtg
     2581 gatcgatcta cccgtgagcc tcttcgcgct cgcagccggc tgcgcgctcg cgttcatgtc
     2641 aggcggtgca ttgctcggct tgctcgtgcc gctcgcggcg tttgccgtcg ccggcaagct
     2701 cggcgtgccg ttcgcgatcg acgtcggccg tgccgcacgc aacggtgcgc tgcgtgtcgc
     2761 gaagcgcgtg gcaccgcggt ttgccgtcgg ccgcgagcca acagcgtacg tcaccggtga
     2821 caactggccg gcattcgtcg cgacgctgtt cacgatcgcc gtcatcggcg tgctcgcgta
     2881 tcagatcttc tcgctcgtcg agccgcagcc cggcatgacg ccgccgcagc gcgcgttgtt
     2941 cggcaaggcg gtcgtgacga ccggctgctg gtcgtcggcg ctcggtggca tctggcgccc
     3001 cgacgacgat ctccgctacg tgttcgactc gacgttcgaa cagatcgcgt acggcacgtt
     3061 cccgtggggc atcctcggca tcgtcgcgat ggctgcgctg ctcgcatcag acgagccgcg
     3121 caagcggctg atcggcgcgc tcacgctcgc gtgggcgggt ggcgcgtgga tcacggccga
     3181 ggtgttccag cgtaaagttg gtttcacgat ctgggcgggc ttcccggcgc tcgcgatcgc
     3241 ggtcggtgtg tggctcgaca gcgcgctcgc cagccgcgcg atggtaccgg cgaaggatcg
     3301 ctcggcgctg cccaaggccg cgatcctcat cggcctgttc gttgcgctcg cgatcgtgaa
     3361 cctcggtaag gacatgcagt cgttctcgga gcgcatctcg tcgttgctca tcggcagcga
     3421 tcagatcgcg tatccgaaga tgtcgaagct gttgtttttg cccacgcgcg tctggatcct
     3481 cgtcgtcggc gcgttggtcg cgaccggctt cgtgctcgcg atgctcgatt tccgccgcgt
     3541 ctggcgtcag ggcatcgcga tcgcgcttgg tggcacggtg ctcgccgcgg cgttctggtc
     3601 gttcggctgg ctgcccgcgc tcgcgaggaa tctgtcgtcg aaggcgatgt tcgacacgta
     3661 cctcgagcta cgaaaacccg gtgaccagct cgtcatcatg ggtgacatgg gcgatgcgcc
     3721 gcacgactac gcgccggacg cgaaggtcga gcagctgacg tcgcgcgatc agatcgtgca
     3781 ggcgattggc cggccgaacc gcgtgttcgc gatcgcgccg cagaccgagc tgtgccagct
     3841 gcatcgcgag atcggcggca aggattattt cgtactcgac gatcgcaaca cgcgctcgct
     3901 gctcttgtcg aacaaggtca ccggcacgac cgataagaat ccgctgcgca ccgccatcct
     3961 gcacagcgaa ccgatcaaca ttccgatcag gccgaagggc aagatcgtgt tcgacggccg
     4021 catcgagcta ctcggctggt cgatgccgaa gcgtgtcggg cgcggcgaca agttcgaggt
     4081 catgatggtc tacaaggtgc tccaacccgt gccgggcaac tggcgcgtgc tgttccactt
     4141 cgacggcgca ctgcgcttca acggcgacca cgagccgatc aagggacgct gccagacctc
     4201 gacctggcag cccggcgatt tcatcgtcga taggtacacg gtgaccgccg gagcgggtgc
     4261 cttcacgccc ggcccctacg acgtctggac cgggttcttc accggaagta atccgaactg
     4321 gaaaaatatg actgtctcgg aagcgcccgc tgacatgcga gatcaggcgg atcgcgtgaa
     4381 gatcacgacg atcaccctcg actgatcgtt gcgattgacg gcgctcgcga cccaggggta
     4441 tgacgcgaga cgatggtcag gtccaagggg gtcaccgcgg aagtgccgac agccgatcat
     4501 gatcggcgtc aggcaccacg tgtgctcgtg gatctcgagg tcgactacgc gagcgaggac
     4561 aactacctgt tcgcgtacat caccgacatc agcgagaccg ggatcttcgt gcggaccacg
     4621 acgccggagc agccgggcac gcacttgaat cttcgctttc gccccgatga tgagggtccg
     4681 cagatcgaag tcgagggcct ggtcatctgg atcaatccgt tccgccccgg tgcaccggac
     4741 aacttgcacc cggggatggg aatccggttc gtcgcgctcg atgacgagct acgcgatcgt
     4801 ttgctggagc tcgtgcgccg cttcgcctat ctaagctgaa agccgatgtc gatcatctgc
     4861 ggtacggacc tcacagctgc tggcgctggc gcactggaag tcgcgcgcgc gctggcagcg
     4921 caacgtggcg atcgcgaggt catcctcgtg cacgtcgccg aagacgaagc gcaagccgag
     4981 agtgcgcgcg caccactcga cgcgcaggcg gcgacgcccg gcatcccggt gcgcgccgag
     5041 ctcgtcgtcg gccagaccga cgagcgcctc gtttcatttg ccgagaccga gggcagcgat
     5101 ctgatcgtga tcgcggcatc ggctggctcg cgcaagctcg gcgcgacggc cgagagggta
     5161 atcgtcgccg cgcgcacgcc ggtgctcgtc gtccgcgacc cggcgccgtg gctcgcgttt
     5221 gcgaagggtg cgaagccgct gcgcgtgctg ctcggcgtcg acgattcggt cgcgtgtgac
     5281 ctcgggatcc agtggacgca cgcattgcgc cagcgcgcac cggtcgaggt cgtgctcggt
     5341 gccatctact accccgacga cgccgccgag tattacggcc tgcatgccgg tgcgatggtc
     5401 gatcgcgacc cggagatcga aaagctggtc gtgcgcgacc tgatgaacaa gttcggcggc
     5461 ggctcgctcg tgcaggcgat cgcgaagcgc ggcctcggcc ggatcggcga tcacgtcgtc
     5521 gagctcggga ccgagacgaa cgtcgacgcg atcatcgtcg gtacgagcca gaagactggc
     5581 ctcggccgcc tcggctcggt atcgcaggtg atcgtccatg acgcgccaca atcggtcgtg
     5641 tgcgtgccgc cgaacgcgaa cgtgccgacg atcagcgtgc cgcggttgac gcgcgcgttg
     5701 gtcgcgaccg atctgtcggc gttcgccaat cgcgcggtcc cgttcgcgtt tgcactgacc
     5761 gacgagggtg gcgaagttca catcgcgcac gtcgtcgacg aggacgccga gatcgacgag
     5821 aacgacgcgc agcgccagct cctcgcgctc gcaccggcga gcatgaagcg ccaggtcgcg
     5881 gcgcacgtcg tgcgcggcag cgacgctgcg cgagcgattg cccaggcggc cgcccggctc
     5941 ggctgtgacg cgatctgcat cgcctcgcat ggccgttcgg gcattacgcg cgcgctggtg
     6001 ggctcggtgg cagacaagct gctcagagca acccgtaagc cggtcctcgt gttgcgcccg
     6061 gcgtaactag ttgcaaggcg gcccgacagt tgtcggtgag ctgttcaccg cgtttcggcg
     6121 cgttttggcg cgcttcgggg cgcgtttcgg cgctgtgcag cgttgcgccc gcccgtgccg
//
LOCUS       DASUFX010000287         4771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1432459, whole genome shotgun sequence.
ACCESSION   DASUFX010000287 DASUFX010000000
VERSION     DASUFX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432459"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFV99_11390"
     CDS             complement(<1..1244)
                     /locus_tag="VFV99_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059957.1"
                     /translation="MSATDDEDGGTGTGGTGTGGTGTIVSLAPRHEITRRQALELIGA
                     GITALTTGCLSRAGDEEIVPYVVDPPEHRPGTPVRYTSALVLDGFACGVVVECNDGRP
                     TKIDGNPAHPASAGGSLPWLQARILDLYDPHRAQAAHLAGTVVGWDAIRGRLRELGTT
                     PLWLVMPPQSSPTLGRLLERLRATREVHVVTDAPLDHRNAYRGAALVYGQPLEQQIDF
                     TRADVVLALDADFLAAMPMSAGWARAFAKRRAPDAHMNRLWSCEPMETATGTLADERL
                     AIAAGDVACVAACVVDELAAHGLAVPRLPGALIAVARGRLAGNAAWVRAAAADLAAHR
                     GAGAVVVGDRQPPAVHALARWIDHACGNVGGPVTLTAPALLDPLGGNTLVELASALHQ
                     GAAAIVIDSDPVYTAPRSLGLA"
     gene            complement(1241..1807)
                     /locus_tag="VFV99_11395"
     CDS             complement(1241..1807)
                     /locus_tag="VFV99_11395"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5059958.1"
                     /translation="MGALYGPGSNAPFRAIAIGVVTFATLAVIAPMVYIRMPYAGRTN
                     AAIAQPVEFDHRHHARDDGIDCVYCHQTVETEAFAGIPATERCMGCHAQVWPESPQLA
                     PVRESWRTGTPLRWRRLNSVPSYVYFHHGVHVQNGVACAECHGAVENMARVMRVHNLT
                     MNFCLDCHRRRQGSRAITRLTTCSTCHR"
     gene            complement(1811..2017)
                     /locus_tag="VFV99_11400"
     CDS             complement(1811..2017)
                     /locus_tag="VFV99_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059959.1"
                     /translation="MTLRLAALLALIPSLALAHPGHGHTDPRSWTHYLTEPVHVAVLA
                     IASVAAVFAMVRARKRSRVIRDRE"
     gene            complement(2027..2278)
                     /locus_tag="VFV99_11405"
     CDS             complement(2027..2278)
                     /locus_tag="VFV99_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059960.1"
                     /translation="MKRKDKKTKVVSNVVVGKPDVEPSTPSHVRGVFEGNHPHVGQRH
                     KGIKEVSEDRAEGTARRSTGIRPKDHDVIDPRMPKISPA"
     gene            complement(2262..4070)
                     /locus_tag="VFV99_11410"
     CDS             complement(2262..4070)
                     /locus_tag="VFV99_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX5059961.1"
                     /translation="MSTTTTPGTGKQRKLVQMAWDPVTRIVGNLGIYTKIDFNNREVV
                     ECKSTSSIFRGYSVFLRGKDPRDAHFITSRICGICGDNHATCSVYAQNMAYGIKMPPL
                     AEWIYNLGEAAEYMFDHAIFQDNLVFVDFCEKMVAETNPSLLAKAERTPAPNSAIHGK
                     RTIADIMRAFNPFTGDFYREALEMSRLTREMFCLMEGRHTHPSTLYPGGTGTVATPQV
                     FTDYLARLIKFVDFAKKLVPLNDDIFNFFYEAIPGYEMVGDRRILLGCWGSFQNPDVV
                     DYRYENMTEWGRAMYVTPGVVVDGKLVTTSLVDINLGIRILLGSSYYEDWASQEMFVT
                     KDPLGNPVDKRHPWNQTTIPKPQKRELEGGNYSWVMSPRWFDKRTNDHLALDTGGGPL
                     ARMWATALAGLVNTPFVRATGKSVIIHLPKTASFGEMDLEWKIPQRANTLERDRARAY
                     FIAYSAAMALYFIDKALERVHSGDVKVFQEFEVPDDAIGCGFHEAVRGVLSHHLVIRD
                     RKIANYHPYPPTPWNASPRDHYGTPGPYEDAVQGMPLFEENGQDNFKGIDIMRTVRSF
                     DPCLPCGVHMYVGDGRVIEQRHAPMFGLGGHDEAEG"
     gene            complement(4067..>4771)
                     /locus_tag="VFV99_11415"
     CDS             complement(4067..>4771)
                     /locus_tag="VFV99_11415"
                     /inference="COORDINATES: protein motif:HMM:NF026070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059962.1"
                     /translation="APKAWAVMAAGTCATYGGIHAMAGNPTGCMGLADYLGWDFRSQA
                     GLPIVNIPGCPIQPDNFMEALVWMLQLAAGKAPIIPLDAKLRPTWLFGKTVHESCDRA
                     GYYEQGDFAVDYNAPRCQVKVGCWGPVVNCNVPKRGWIGGVGGCPNVGGICIGCTMPG
                     FPDKFMPFMDQPPGAAFSSKLVTTYGRVVRALRGITNHTMDEEPKWHHNGPALTSGYD
                     PYYSPGSNGRRKELTR"
BASE COUNT          769 a         1559 c         1586 g          857 t
ORIGIN      
        1 ctcgcaagtc cgagcgaacg tggcgccgtg tacaccggat cgctgtcgat cacgatcgca
       61 gcggcaccct gatgcagcgc ggaggcgagc tcgacgaggg tgttgccgcc gagcggatcg
      121 aggagcgccg gtgcggtcag cgtgactggg ccgccaacgt tgccgcatgc atgatcgatc
      181 cagcgtgcga gtgcgtgcac ggcgggcggt tggcgatcgc cgacgaccac cgcacccgcg
      241 ccgcgatgcg cggccagatc ggctgccgcg gcgcgcaccc aggccgcgtt gccggcgagc
      301 cgcccgcgcg ccaccgcgat caacgcacca ggcagccgcg gtacggcaag cccgtgcgct
      361 gccagctcgt cgacgacgca cgccgcgaca cacgcgacgt cgcccgccgc gatcgcgagc
      421 cgctcgtcgg cgagtgtgcc agtcgcggtc tccatcggct cgcacgacca cagccggttc
      481 atgtgtgcat cgggagctcg ccgtttcgcg aacgcgcgcg cccagccggc tgacatcggc
      541 atcgcagcga ggaagtcggc atcgagcgcg agcacgacgt cggcgcgcgt gaaatcgatc
      601 tgctgctcga gcggctgccc gtaaacgagt gcagccccgc gatacgcatt gcgatgatcg
      661 agtggcgcgt cggtgacaac gtggacttcg cgggttgcgc gcagccgttc gaggagccgc
      721 ccgagcgtcg gcgatgattg cggtggcatc acgagccaca acggggtcgt gccgagctcg
      781 cgcagcctgc cgcgtatcgc atcccaaccg acgacggtgc ccgcgaggtg cgcggcctgc
      841 gcgcggtgcg ggtcgtagag gtcgagaatg cgtgcctgca accacggcag cgagccgccg
      901 gcactcgccg gatgcgctgg gttgccgtcg atcttcgtcg gccggccgtc gttgcactcg
      961 acaacaacac cgcacgcgaa gccgtcgagg acaagcgcgc tggtataccg gaccggcgtt
     1021 cccgggcgat gttccggcgg atcgacgacg tagggcacga tctcttcgtc gcccgcacgt
     1081 gacaagcagc cagtcgtcag tgcagtgatg cctgcgccga tcagctcgag cgcctgccgc
     1141 cgcgtgatct cgtgacgcgg tgcgagcgac acgatcgttc cggtcccccc cgttccggtc
     1201 ccccccgttc cggttccccc gtcttcgtcg tccgtcgcgc tcatcggtga cacgtagagc
     1261 aagtggtgag ccgggtgatc gcgcggctgc cttgccgccg ccggtgacag tccaggcaga
     1321 agttcatcgt caggttgtgc acgcgcatca cgcgtgccat gttctcgacg gcgccgtgac
     1381 actcggcgca ggcgacgccg ttctgcacgt gcacgccgtg gtggaagtag acatacgagg
     1441 gaaccgagtt gagccgtcgc cagcgcagcg gtgtacccgt gcgccagctc tcgcgaacgg
     1501 gcgcgagctg tgggctctcc ggccacacct gggcgtggca gcccatgcaa cgctcggtcg
     1561 ctggtatgcc agcgaacgct tcggtctcga ccgtctggtg gcagtagacg cagtcgatgc
     1621 cgtcgtctcg cgcatggtgt cggtgatcga attcgaccgg ctgcgcgatc gcggcgttgg
     1681 tacggccagc gtatggcatc cggatgtaga ccatcggtgc gatcactgcg agcgtcgcaa
     1741 acgtgacgac gccgatcgcg atggcgcgaa acggcgcatt cgaaccggga ccgtacaacg
     1801 cccccacagg tcactcgcgg tcgcggatga cgcgcgaccg tttgcgcgcg cgaaccatcg
     1861 caaataccgc agccaccgac gcaatcgcca gcacggcgac gtgcacgggc tcggtgaggt
     1921 aatgcgtcca gctccgagga tcggtatgac cgtgcccggg gtgtgcgagc gcgagactgg
     1981 gaatcagcgc gagtagcgcg gcgagtcgta gcgtcacgtg gcctccttag gcgggtgaaa
     2041 tcttcggcat gcgtggatcg atcacgtcgt gatctttggg acggattccg gtcgagcgcc
     2101 gcgcggtgcc ttccgcgcgg tcctccgaca cctccttgat gcctttgtgg cgttgtccga
     2161 cgtgcgggtg attcccctcg aacacgccgc gcacgtggct cggcgtcgac ggctccacgt
     2221 ctggtttgcc gacgacgacg ttcgagacga ctttcgtttt cttatccttc cgcttcatcg
     2281 tgtcctccga gaccgaacat cggcgcatga cgctgctcga tcacgcggcc gtcgccgacg
     2341 tacatgtgca cgccgcatgg cagacacggg tcgaagctgc gcacggtgcg catgatgtcg
     2401 atgcctttga agttgtcctg accgttttcc tcgaacagcg gcatgccttg caccgcgtcc
     2461 tcgtacggac ctggtgtgcc gtagtggtca cgcgggctcg cgttccatgg cgttggcggg
     2521 tacgggtgat agttcgcgat cttgcgatcg cggatcacga ggtgatgcga gagcacgcca
     2581 cgtaccgcct cgtgaaagcc gcagccaatc gcgtcgtccg ggacctcgaa ctcctggaac
     2641 accttgacgt cgccggagtg cacgcgctcg agcgccttgt cgatgaagta cagcgccatc
     2701 gccgccgaat acgcgatgaa gtacgcgcgc gcgcgatcgc gttcgagcgt gttcgcacgc
     2761 tgtgggatct tccattcgag atccatctcg ccgaacgacg ccgtcttcgg caggtggatg
     2821 atcacgctct tgccggtcgc gcgcacgaat ggtgtgttga cgaggccggc gagcgcagtc
     2881 gcccacatgc gtgcgagcgg gccaccgccg gtatcgagtg cgagatgatc gttcgtgcgc
     2941 ttgtcgaacc agcgcgggct catcacccag ctgtagttgc cgccttcgag ctcacgcttc
     3001 tgcggcttgg ggatcgttgt ctgattccac ggatgccgct tgtcgacggg attgccgagc
     3061 ggatccttgg tgacgaacat ctcctggctc gcccagtcct cgtagtacga gctgccgagc
     3121 aggatccgga tcccgaggtt gatgtcgacc aggctcgtcg tgacgagctt gccgtcgacg
     3181 acgacaccgg gggtcacgta catcgcacgg ccccactcgg tcatgttctc gtagcggtaa
     3241 tcgacgacat cggggttctg gaacgagccc cagcagccga gcaggatgcg gcgatcaccg
     3301 accatctcgt agccgggtat cgcttcgtag aagaagttga agatgtcgtc gttgagcggg
     3361 acgagcttct tcgcgaagtc gacgaacttg atcaagcgcg ccaggtaatc ggtgaacacc
     3421 tgcggtgtcg cgacggtgcc ggtgccgccg gggtacagcg tcgatggatg cgtgtgccgg
     3481 ccttccatca ggcagaacat ctcgcgtgtg agccggctca tctcgagcgc ttcacgatag
     3541 aagtcgcccg tgaacggatt gaacgcgcgc atgatgtccg cgatcgtgcg cttgccgtgg
     3601 atcgccgaat tcggcgccgg cgtgcgctcg gccttcgcga gcagcgacgg attcgtttcg
     3661 gccaccatct tctcgcagaa gtcgacgaac accaggttgt cctggaagat cgcgtggtcg
     3721 aacatgtatt cggcggcttc gccgaggttg tagatccact ccgcgagcgg cggcatcttg
     3781 atgccgtacg ccatgttctg cgcgtacacc gagcacgtcg catggttgtc gccgcagatg
     3841 ccgcagatgc gactcgtgat gaagtgcgcg tcgcgcggat ctttgccgcg caagaacacg
     3901 ctgtagccgc ggaagatcga cgatgtgctc ttgcactcga cgacttcgcg attgttgaag
     3961 tcgatcttcg tgtagatgcc gaggttgccg acgatccgcg tgaccggatc ccacgccatc
     4021 tgcaccagct tgcgttgctt ccccgtcccc ggcgtagtgg tcgtgctcat cgcgtcagct
     4081 ccttgcgccg gccgttgcta cccggcgagt agtaggggtc gtagccgctc gtcagcgccg
     4141 gaccgttgtg gtgccacttc ggctcttcat ccatcgtgtg gttcgtgatc ccgcgcagcg
     4201 cgcgcacgac acggccgtat gtcgtgacca gcttcgacga gaacgccgcg cccggcggct
     4261 gatccatgaa cggcatgaac ttgtcgggga agcccggcat cgtgcagccg atgcagatgc
     4321 cgccgacgtt ggggcaacca ccgacgccgc caatccaccc gcgcttgggc acattgcagt
     4381 tgacgaccgg tccccagcac ccgaccttca cctggcagcg gggggcgttg taatcgaccg
     4441 cgaagtcgcc ttgctcgtag tacccggcgc gatcgcagct ctcgtgcacg gtcttgccga
     4501 acagccacgt cggccgcagc ttcgcgtcga gcggaatgat cggcgccttg ccggcggcga
     4561 gctggagcat ccagaccagc gcctccatga agttgtcggg ctggatcgga caaccgggga
     4621 tgttgacgat cggcagaccc gcctgcgagc ggaagtccca gccgaggtag tcggcaagcc
     4681 ccatgcagcc ggtcgggttg ccggccatcg cgtggatgcc gccatacgtc gcgcaggttc
     4741 ccgctgccat cacggcccac gctttcggcg c
//
LOCUS       DASUFX010000288         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_739387,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000288 DASUFX010000000
VERSION     DASUFX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739387"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFV99_11420"
     CDS             <1..726
                     /locus_tag="VFV99_11420"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5059963.1"
                     /translation="DTHTVFDFAPGFAAARDLATQGFGVMAIDRVGYGASSHPNGDTL
                     SFATHAGYVHEVVQDVRAGALGFTPPAVILLGPSVGADVTMVEAATFHDVDGVVIVAN
                     TNALQPALFQVDVNAWFAQGPYFDFGVDFRTSFFYAEPWALQWIIDQDNATRALVPRA
                     EILSALTGQSAFARALIDVPVLLMQADHDHLFVPQDDSALFTSSPDVSFVLLKNTGHK
                     LFSHPTSKAAAVQTVGNWLDSRF"
     gene            complement(723..1019)
                     /locus_tag="VFV99_11425"
     CDS             complement(723..1019)
                     /locus_tag="VFV99_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059964.1"
                     /translation="MTRFANLTRSQLRRRAILFTTMWLSWLYLGDYIASFVPHAAQIP
                     VMLIAMVPFVVAGRYGIELWYRAKTTPELPHAEVVSHADIVSREAVSRPRDRST"
     gene            complement(1038..1436)
                     /locus_tag="VFV99_11430"
     CDS             complement(1038..1436)
                     /locus_tag="VFV99_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059965.1"
                     /translation="MECTNCRTSVYSCSRCGAETCVDHMALEHRDTCLDCALAFYDSQ
                     DKLHLNAWFIAGFILPWALYLRMLPDLPSWSERSGGYRAMTTGFPALDVLIIVGFVSV
                     FAGKAMIGLRKWMHRRTFVTRELARAKLVR"
     gene            complement(1555..2298)
                     /locus_tag="VFV99_11435"
     CDS             complement(1555..2298)
                     /locus_tag="VFV99_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5059966.1"
                     /translation="MGNDRVDQLIALGREHYNANEYERAEPFLAEAASARPSFPDLYN
                     MLGVIYHAQGRFSDAEEAFENALRLNPRYTEAALNLSVTYNDRGKYDRAREVYTRAVS
                     ASVGQPNALDRFARGKLANMHADLGAAYAGLAMFDEAVREYSKSLDLCPDFADLRVRL
                     GNVYRDMGMFHAAVAEFEHAKKLRPDFVPARLHLGVTMFSLGRRDAAIAEWQEVLRID
                     PTNKNAQLYIRMVNDENGPQPGSALPNRS"
     gene            complement(2350..>2990)
                     /gene="bamD"
                     /locus_tag="VFV99_11440"
     CDS             complement(2350..>2990)
                     /gene="bamD"
                     /locus_tag="VFV99_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5059967.1"
                     /translation="AKYFGFIKSRFPYSKYAVLAELRLADAEFGAEQYLESIDSYRLF
                     IKFHPTHEMVANGYASFRIGEAYYKQLPGDFWLFPPSYEKDMSSTEDASTELKSFLTK
                     YPDSPYRERAQEIVKKVGKRLADHEWYVARYYWDRGKPMGTVLRLRRLLEHYRGVGYD
                     EEALWLLGRAYVAVDMPDRARGSWTELVQKFPKSDRASDARDALASLAPAKR"
BASE COUNT          502 a          961 c          994 g          533 t
ORIGIN      
        1 gacacgcaca cggtgttcga cttcgcgccc gggtttgcgg ccgcgcgcga tctcgccacc
       61 caagggttcg gtgtgatggc gatcgatcgc gtcggctacg gcgcgtcgag ccatccgaac
      121 ggcgacacgc tgtcgttcgc aacgcacgcc ggctacgtcc acgaggtggt gcaagacgtg
      181 cgcgccggtg cgctcggctt cacgccgccg gcggtcattc tgctcgggcc ctcggtcggt
      241 gctgacgtca cgatggtcga ggcggcgacg ttccacgacg tcgatggtgt cgtcatcgtc
      301 gcgaacacga acgccctgca acccgcgctg ttccaggtcg acgtcaatgc ctggttcgcg
      361 caaggcccgt acttcgactt cggcgtcgac ttccgcacga gcttcttcta cgcagagccg
      421 tgggcgctgc aatggatcat cgatcaggac aatgcgacgc gcgcgctcgt gccgcgggcc
      481 gaaatcctct cggcgctcac cggtcaatcg gcgttcgcgc gcgcgttgat cgacgtgccg
      541 gtgctcctca tgcaagcgga ccacgatcat ctgttcgtgc cgcaggatga ctccgcgctg
      601 ttcacgtcgt cgccggacgt gtcgttcgtg ctgctcaaga acaccggcca caagctgttc
      661 tcgcacccga cgagcaaggc ggccgcggtg cagaccgtcg gcaactggct cgacagccgg
      721 ttctaggtcg atcgatcgcg cgggcgcgag acggcttccc gcgaaacgat gtcggcgtgc
      781 gagacgacct cggcgtgcgg aagctccggg gtcgtcttcg cccgatacca cagctcgatg
      841 ccgtaccggc ctgcgacgac gaacggcacc atcgcgatca gcatgaccgg aatctgcgcg
      901 gcatgcggca cgaacgacgc gatgtagtcg ccgaggtaca gccacgacag ccacatcgtc
      961 gtgaacagga tcgcgcgtcg tcgtagctgg ctgcgcgtca ggttggcgaa tcgggtcatg
     1021 actgcgttac ggcgggttca gcgtactagt ttcgcgcgag caagctcgcg agtcacgaac
     1081 gttcggcggt gcatccattt gcgcaggccg atcatcgcct tgcctgcgaa caccgacacg
     1141 aagccgacga tgatcagcac gtcgagggca gggaagccgg tcgtcatcgc gcggtagccg
     1201 ccggaccgct ccgaccagct cggcagatcg ggcagcatgc gaagatagag cgcccacggc
     1261 aggatgaagc ccgcgatgaa ccacgcgttc agatgcagct tgtcttggct gtcgtagaac
     1321 gcgagcgcgc agtcgaggca ggtgtcgcga tgctcgagcg ccatgtgatc gacgcacgtc
     1381 tcggccccac accgcgagca gctgtacaca ctcgtgcggc agttggtgca ttccatggtg
     1441 gacgctcctt tcgacggcac gccatcaacg tggcgctgcg atcttcgatg cgccaggtct
     1501 tgccgaccgg ttcatccgtt ggcgcggaga tgcgtctacc cgcgcgggcg ctggctaact
     1561 gcggttgggc aacgcggatc ccggctgcgg accgttctcg tcgttgacca tgcggatgta
     1621 gagctgcgcg ttcttgttcg tcggatcgat gcgcagaacc tcttgccact cggcgatcgc
     1681 ggcgtcgcgg cggccgagcg agaacatcgt gacgccgagg tgcaaacgcg ccggcacgaa
     1741 gtcggggcgc agcttcttgg catgctcgaa ctcggcgacc gccgcgtgga acatgcccat
     1801 gtcgcgatag acgttgccga gtcgcacgcg caggtccgcg aagtcgggac acagatcgag
     1861 cgacttcgag tactcgcgca cggcttcgtc gaacatcgcg aggccggcgt atgcagcgcc
     1921 gaggtcggcg tgcatgttgg cgagcttgcc gcgcgcgaat cgatcgagcg cgttcggctg
     1981 gccgaccgat gccgacacgg cacgcgtgta cacctcgcgc gcgcggtcgt acttgccgcg
     2041 atcgttgtac gtgacgctca aattcagcgc ggcctcggtg tagcgcgggt tcagccgcag
     2101 cgcgttctcg aacgcttcct ccgcatcgga gaaccggccc tgcgcgtgat agatcacgcc
     2161 gagcatgttg tagagatcgg ggaagctcgg ccgtgccgat gccgcctcgg cgagaaacgg
     2221 ctcggcgcgc tcgtactcgt tggcgttgta gtgctcgcgc ccgagcgcga tcagctgatc
     2281 gacccgatcg tttcccatca cggctagcgt atcccaggcc gctgccaact actaacgctc
     2341 gcgagatcgc taacgctttg cgggggcgag gctggcgagc gcgtcccgtg catcgctcgc
     2401 acgatcgctc ttcggaaact tctggacgag ctcggtccac gaaccgcgtg cacgatccgg
     2461 catgtcgacg gcgacgtacg cgcggccgag tagccatagc gcttcttcgt cgtagccgac
     2521 accgcgatag tgttcgagca agcggcgcaa ccgcagcacg gtgcccatcg gcttgccgcg
     2581 atcccagtag tagcgcgcga cgtaccactc gtggtctgcg agccgcttgc cgaccttctt
     2641 gacgatctcc tgggcgcgct cgcggtacgg cgagtcggga tacttcgtga gaaagctctt
     2701 cagctcggtc gaggcatcct cggtcgagga catgtccttc tcgtaggacg gcgggaacag
     2761 ccagaagtcg ccggggagct gcttgtagta cgcctcgccg atgcggaacg acgcgtagcc
     2821 gttcgcgacc atctcgtgcg tggggtggaa cttgatgaac agacggtagg agtcgatcga
     2881 ctcgaggtat tgctcggcgc cgaactcggc gtcggcgagg cggagctcgg cgagcaccgc
     2941 gtacttcgag tacgggaagc ggctcttgat gaagccgaag tacttcgccg
//
LOCUS       DASUFX010000289         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1573906, whole genome shotgun sequence.
ACCESSION   DASUFX010000289 DASUFX010000000
VERSION     DASUFX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573906"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2166
                     /locus_tag="VFV99_11445"
     CDS             <1..2166
                     /locus_tag="VFV99_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5059968.1"
                     /translation="AGAGTGKTRVLVHRIARLVESGVEPWAILAVTFTNKAAGEMRHR
                     LRGLLGARADAMWIGTFHATCARLLRKYAGIVGLTKSFVIFDDDDQMKLVEKLLKETG
                     LDEQISTRTVLSRFDRAKNRGVDPRSVKTGAFDDVIEKIYPLYQAQLAKENAVDFNDL
                     ILKTLDLFKHEETARALQVRFRHVLVDEFQDTNRVQYDLVSKFAEATRNLTVVGDDDQ
                     SIYAWRGAEPRNLLDFDRDFPDATVVKLEQNYRSTQMILDAANGVIKKNRDRHEKQLW
                     TDHKGGDQIEVYQAGDVRGEAYFVAQSIRRMLDEGPYSPSDVAILYRTNAQSRVLEEH
                     LRAARVPAKVVGAVSFFERKEVKDVIGYLRLLGNPSADSAFERIVNVPARGIGDATVD
                     RLRGAARAGLPGLLEAARLAARGEVAGIGPAPRKKLQAFVELLDGLRDVIAQGASVAE
                     TIIQVVDRSGLRAKLEADESSESRDRLDNLAELVTIASDFDEESDEAQSVDGFLERIA
                     LTAPADQSTTGETVVLMTIHIAKGLEWPVVFLTGMEDGLFPSLREREGVNEDAALEEE
                     RRLAYVAITRARTRLIFTHARTRRVWGEIRLQGPSRFLDDVPAGCLAGPARPKVITPK
                     APAIVDGNWNARPRRRPRASIDEFDQRLPDDEPVYRVDDDLEEPVVFRTGDQVAHQLL
                     GIGRVIAMSDGKVIVEFPGAGRKTVLPKFLALAPDDGLN"
     gene            2304..2726
                     /locus_tag="VFV99_11450"
     CDS             2304..2726
                     /locus_tag="VFV99_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059969.1"
                     /translation="MRIGLALLVVLSVAAPASADSKVKNLIKFYNKEAMVCAKYAQGI
                     ASAKERAEPYVDDAEIKADVDKLGQVQVVVRDYCDAIDATIAILDPNASYKSLQAEID
                     KHDAVVRAGREATKDALEESNVVIQRLIPKVNKRRANS"
     gene            complement(3032..>3263)
                     /locus_tag="VFV99_11455"
     CDS             complement(3032..>3263)
                     /locus_tag="VFV99_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5059970.1"
                     /translation="QKISGALVTGVEPSSPAIRAALRAGDVILKWDGREVDHRTLPWA
                     VAATPPGKNVNVVIWRNRVEMPFVVVTEKMPQ"
BASE COUNT          598 a         1025 c         1112 g          528 t
ORIGIN      
        1 gccggtgccg gcacgggcaa gacccgcgtg ctcgtgcacc gcatcgcgcg gctcgtcgag
       61 agtggcgtcg agccgtgggc gatcctcgcg gtcacgttca cgaataaagc ggctggcgag
      121 atgcgccacc ggctgcgcgg actgctcggc gcgcgtgccg atgcgatgtg gatcggtacg
      181 ttccacgcca cgtgcgcgcg gctgttgcgc aagtacgcgg gcatcgtcgg gctgacgaag
      241 agcttcgtga tcttcgacga cgacgatcag atgaagctgg tcgagaagct gctcaaggag
      301 accgggctcg acgagcagat cagtacgcgc accgtgctgt cgcgcttcga tcgcgcgaag
      361 aaccgcggcg tcgatccgcg atcggtgaag accggcgcgt tcgacgacgt gatcgagaag
      421 atctatccgc tgtaccaggc gcagctcgcg aaggagaacg cggtcgattt taacgacctc
      481 attctgaaga cgctcgatct gttcaagcac gaagagacgg cgcgcgcgct ccaggtcagg
      541 ttccggcacg tgctcgtcga cgagttccag gacacgaacc gcgtccagta cgacctggtt
      601 agcaaattcg ccgaagctac gcgcaacctc acggtcgtcg gtgatgacga tcagtcgatc
      661 tatgcgtggc gcggtgcgga gccgcgcaac ttgctcgact tcgatcgcga ctttcccgac
      721 gccaccgtgg tcaagctcga gcagaactat cgttcgacgc agatgatcct cgacgcggcg
      781 aacggcgtga tcaagaagaa ccgcgatcgc cacgagaagc agctgtggac cgatcacaag
      841 ggcggcgatc agatcgaggt ctatcaggca ggtgacgtgc gcggcgaggc gtacttcgtc
      901 gcgcagtcga tccgccggat gctcgacgag gggccgtatt cgccgagcga cgtcgcgatc
      961 ctctaccgca cgaacgcgca gtcgcgcgtg ctggaagaac atctgcgcgc cgcgcgcgtg
     1021 ccggccaagg tcgtcggcgc ggtgtcgttc ttcgagcgca aggaagtaaa ggacgtcatc
     1081 ggttacctgc gtctgctcgg caatccatcg gccgactcgg cgttcgagcg catcgtcaac
     1141 gtgccggcgc gcggcatcgg cgatgcgacg gtcgatcggc tgcgtggtgc ggcgcgtgcg
     1201 gggctgccgg gcctgctcga ggcggcacga cttgccgcgc gcggcgaggt ggcgggtatc
     1261 ggacctgcgc cgcgcaagaa gctgcaggcg ttcgtcgagc tactcgatgg cttgcgcgac
     1321 gtgatcgcgc agggtgcgtc ggtcgccgag acgatcatcc aggtcgtcga tcgcagcggt
     1381 ctgcgcgcga agctcgaagc ggacgagagc agcgagtcgc gcgaccgcct cgataacctg
     1441 gccgagctcg tcacgatcgc gtcggacttc gacgaggaga gcgacgaggc gcagagcgtt
     1501 gacggcttcc tcgagcgcat cgcgctgacc gcgccggcag atcaatcgac gaccggcgag
     1561 acggtcgtgc tgatgacaat ccacatcgcg aagggcctcg agtggccggt cgtgttcttg
     1621 actggcatgg aggacggcct gtttccatcg ctccgcgagc gtgagggcgt caacgaggat
     1681 gccgcgctcg aggaggagcg ccggctcgcg tacgtcgcga tcacgcgtgc gcgcacccgg
     1741 ctcatcttca cgcacgcgcg cacccggcgg gtgtggggcg agattcgctt gcaggggccg
     1801 tcgcgattcc tcgacgatgt gccggcgggc tgcctcgcag gcccggcacg gccgaaggtc
     1861 atcacgccga aggcaccggc aatcgtcgac ggcaactgga acgcgcgccc ccggcggcgg
     1921 ccgcgcgcaa gtatcgacga attcgaccag cggcttccgg atgacgagcc ggtgtaccgc
     1981 gtcgatgacg acctcgagga gcccgtcgtc ttccgcaccg gagatcaggt tgcccaccag
     2041 ctgctcggca tcgggcgagt gatcgcgatg agcgacggca aggtgatcgt cgagttcccc
     2101 ggcgccggcc gtaaaaccgt gttgccgaaa ttcctcgcac ttgcgcccga cgacgggctc
     2161 aactgaccga cggctcggaa gcggcctcac agttcctgct ggtgctgcca ctaaccggac
     2221 cggccaattg ctgtcgacgt aggtgagcgg ttgcgagcag ggagtgtggg ggaatcggga
     2281 agctcgcgat cgacgataca gccatgagga tcggactagc tctgctcgtg gtgctcagcg
     2341 tcgcggcgcc tgcgtccgct gactcgaagg tcaagaactt gatcaagttc tacaacaagg
     2401 aagcgatggt ctgcgcgaag tacgcgcaag gcatcgcgag cgcgaaggag cgcgccgagc
     2461 cgtacgtcga cgatgcggag atcaaagccg atgtcgacaa gctcggtcag gtgcaggtag
     2521 tcgtgcgcga ctactgcgat gcgatcgatg cgacgatcgc gatcctcgat ccgaacgcga
     2581 gctacaagtc gctgcaggcc gagatcgaca agcacgacgc ggtcgtgcgc gccggacgcg
     2641 aggcgacgaa ggatgcgctc gaggagagca acgtcgtcat ccagcggttg atcccgaagg
     2701 tcaacaagcg ccgcgcgaac agctgatcaa ccgccaagac gccgcagacg ccaagatcgc
     2761 cgcatccgaa gctccgatca tgcgtatccc atgcacgcgc ggtcgtcgcc ctcatcaacc
     2821 gccaagacgc cgcagacgcc aagcccagag attcgattca ttcgaaacac cacccttcgt
     2881 acgcgttcgc ccgaacggcg gcacagggct gtgatcagat tcaaggcctg atcttggcgt
     2941 cggcggcgcc ttcgcggttc gtctcttctg ggggacgcac caacaactgc gctcgatcgt
     3001 ctgggctgtg tccgcggcgg cttggcggtc gctactgcgg catcttctcg gtcacgacga
     3061 cgaacggcat ctcgacgcgg ttgcgccaga tcacgacgtt gacgttcttg ccaggaggcg
     3121 tcgccgcgac ggcccacggc aacgtgcggt gatcgacttc gcggccatcc catttgagaa
     3181 tgacgtcgcc agcgcgcagt gctgcgcgga tcgccggcga gctcggctcg acgccggtga
     3241 cgagtgcgcc gctgatcttc tgg
//
LOCUS       DASUFX010000290         7399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_879366,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000290 DASUFX010000000
VERSION     DASUFX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879366"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFV99_11460"
     CDS             complement(<1..686)
                     /locus_tag="VFV99_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059971.1"
                     /translation="MKALAALATSNGQSVHDLWVGQGHMTETQLASVLSALGKSSETI
                     VMSDVQTTPRVPKKPEDLVTNVGHKARAKMPTQQATAPTAASIVNEPPPPDMALLDPG
                     TDRLGPAALSNMLGGRYKQMSVLGKGGLGEVMECDDTVLQRTVAVKAGHLKGDIDAYS
                     TKVILAREARIISCLEHPNIIPIYDAGTDNVRGPFYVMRKVTETSLEMILHQRKTGEG
                     NPHDYTLNRLL"
     gene            complement(756..2552)
                     /locus_tag="VFV99_11465"
     CDS             complement(756..2552)
                     /locus_tag="VFV99_11465"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5059972.1"
                     /translation="MKLRTTVFLWVLLLVVAVLGATIGTIAYVFDRSTRTRVANDLAR
                     SRVVTLDQHSSRASLLRQECRVVAEEPRLKAVVATEDVARDTILDAVRTLAETLHSGV
                     FVIVDADGRLIADNADPQAAGDDMTHQPVVANALAKGEESGVWLADNKAFQVQGCRLE
                     FGARVVGALVVGHAIDDDFAATVARQTGGKLTVVDDHAALTSRPEAISAEELRTGLVA
                     IRGGEREVELGGTHWYAQLVAVPGYHGESRVEYLLLHSIDEALAPGRKIIRILLLLLA
                     GAALATLVLALGLARRLSRPIDALVTRTQAIAQGDLSPRSVGGPTEVQALGTAMNQMA
                     KEIDDSRMALADKERLARELEIAARIQTSILPRNMNVDGLEIAARMMTASEVGGDYYD
                     VLPIPTGCWIAVGDASGHGLTSGLVMMMVQTGVATLVRAEPMAKPSFVLKTLNRVLFE
                     NVQERLEAERHMTLSLLRYDRNGTLVVAGAHMDAVCWRAATQKTEMLRTPGTFLAITD
                     DIDHVNIEQQWSLAAGDLLILLTDGVTEAEDKTGKPFGYEGVLETVEARATQPVAVIQ
                     NALFDAVTKHSPTLADDCTIVVLRYVGLGESS"
     gene            complement(2549..3658)
                     /locus_tag="VFV99_11470"
     CDS             complement(2549..3658)
                     /locus_tag="VFV99_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5059973.1"
                     /translation="MAVVADAAESKVFVTGAGRCGECHEKMFDEWEVSAHAKAVSSSF
                     YKVSVAASKDPSCDRCHAPLAKVVPRDITVSEGVTCDVCHTLREPQPSPDGGKFRLAI
                     DDMVKYGPRCDLKDHYFHRMGCSKEHQQAAICGTCHWWEPKGIPVFTEYADWKAGPEA
                     AKGMQCQDCHMPKERAALAVGSSVRSGVPHHGLLGLAQDLRKRALGLDVTVKDDAGSL
                     AVAVTVRNNNAGHAVPAGLPERRIIVRARVLDAAGAQVAADTRALGRVLVDASGAEVP
                     FWRATKVGTDTRIAVGGSWQDTFKLAAANIGTLEVDVTYRGIADSIAKELGITEIEEQ
                     AMASAKIRLGGALPKTVSVKPPPPGKRVAKKGPTP"
     gene            complement(3754..4422)
                     /locus_tag="VFV99_11475"
     CDS             complement(3754..4422)
                     /locus_tag="VFV99_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5059974.1"
                     /translation="MRRLPIIVMFAALVVFVGDARSKPSTGSVRGTVTINAKGKAKSD
                     KKGVVVYLEGVAGTPPVPKDHAIIRQREKQFEPPLTIVVKGTTIDFPNEDKIFHNVFS
                     VSRPARFDLGLYKSGTAKSVEMKRSGTVDVYCNIHPEMIAKVKVLDNAYYTITGADGA
                     FRIDNVPAGEYPVVAWLPTGDEAKGTVTVKAGEAAEVKLEMSEVAKKETHTRKDGTPY
                     GRYK"
     gene            complement(4431..5864)
                     /locus_tag="VFV99_11480"
     CDS             complement(4431..5864)
                     /locus_tag="VFV99_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5059975.1"
                     /translation="MKRIFLASLIVATPAWAQPADPQPQPAQPAQPEPPTQTEPKEPA
                     EPKVDLKLHELEQRLQKVEDELEQAKDDNSYLEEKLQSLLPLTGKISGYVDMGAFVTS
                     GNGAGTRTDLLQTWFPEFIDKVPGSWVFYGDPLSTAINSRGDVADTGESRAVTFDPIN
                     SHGKSTMLVNAVNLALFSGIGETAQLNASIDFVPRARDVSDPAGLFVGDYIDVKLAYA
                     EWRPRIEKFDLMLQAGKFDSVVGYEYRSLESPDRVGVTPSLICRYTCGRPTGLKARAR
                     FLDEALILNVAVTNGSHFSEGFPFSSETDTNQMKTGAGRLSYQIAKVVEFGVSGAWGA
                     QDFQPFNDVYQWHYGADMHIDWKDLEVTAEYVHGRAKGKTSPMAMAECDVAPCLNYKG
                     AYGLVAYRVTNMFVPYIRVDWRDAYHYSGASFVYIADLARATLGLRAEIGTRVIVKIE
                     GTKNRELEKDVPQIPNDVLTSSLVIKY"
     gene            complement(5861..6727)
                     /locus_tag="VFV99_11485"
     CDS             complement(5861..6727)
                     /locus_tag="VFV99_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059976.1"
                     /translation="MLPTVLPDRLSQTGLYTNIRTKTLSPRLHEFTPNNVLWSDAAEK
                     HRWYQLPTGATIDTTDMNHWKFPVGTKFFKEFSLDGKRLETRLIWRVADTGDLERDTL
                     AGSYVWDDNESDAEFAKDGAQNLRGTEHDAPPADMCWRCHIGEPGHSLGMSALQLGDV
                     SALPLSSPPPAGTTYEAPNPALGYLHANCGHCHNPNGSAWADSHMILRLDVDEHDAAT
                     TQIVQTTVGVPLEQWVGHGYVHRIVAGDPDMSAAFFRITQRTMNVQMPPIATEKTDDT
                     GIALIRSWIQSL"
     gene            complement(6811..>7399)
                     /locus_tag="VFV99_11490"
     CDS             complement(6811..>7399)
                     /locus_tag="VFV99_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059977.1"
                     /translation="DSEIPMPAGGANNESAMRGRYLSTQVGLCIDCHSPTLPGSFLEF
                     DKSKFFGGGRVFTQEQLGLKDVAFPPAIATRNITPEAFPKGVGGWTKQQISDAIAKGK
                     DTDGKQVCAATHGGLVSGYAALEPQDLADIVEYLYTIPAIDNDGALANCGPPPIAAGP
                     ETDAQCGNFTDDDGDQVPDDGCYIPCGNCQGPPVP"
BASE COUNT         1193 a         2436 c         2397 g         1373 t
ORIGIN      
        1 agcaggcggt tgagcgtgta gtcgtgcggg ttgccctcgc cggtcttgcg ctggtgcaag
       61 atcatctcga gcgacgtctc cgtgaccttg cgcatcacgt agaacggacc acgcacgttg
      121 tcggtgcccg cgtcgtagat cgggatgatg ttcgggtgct cgaggcagga aatgatccgc
      181 gcctcgcgcg cgaggatcac cttcgtcgag tacgcgtcga tgtcgccctt gaggtggccg
      241 gccttcaccg cgacggtgcg ttgcagcacc gtgtcgtcgc attccatcac ctcgccgagg
      301 ccgcccttgc cgagcaccga catctgcttg tagcgaccac cgagcatgtt cgacagcgcg
      361 gcaggaccga gccgatcggt gccgggatcg agtagcgcca tgtcgggcgg cggcggctcg
      421 ttcacgatcg atgctgcggt cggcgccgtc gcctgctgcg ttggcatctt cgcgcgcgcc
      481 ttgtggccga cgttcgtgac caggtcctcg ggcttcttcg gcacgcgcgg cgtcgtctgc
      541 acgtccgaca tcacgatcgt ctcgctgctc ttgccgagcg ccgagagcac cgaggcgagc
      601 tgggtctcgg tcatgtgacc ttggccgacc cacaggtcat gcaccgactg accgttcgac
      661 gtcgcgagcg cagcaagggc cttcacgaac gcgtcgtgcg ttatgaatcc gcgcgaaagc
      721 gcggcgatcc ccacgttgat gtggatctca gccattcacg aggactcccc cagaccgacg
      781 taacgcagca caacgatagt acagtcatca gccaacgtcg gggaatgctt ggttacggcg
      841 tcgaacagtg cgttctggat cacggccacc ggttgcgtcg cgcgcgcctc gacggtctcc
      901 agaacccctt cgtaaccgaa cggtttgccg gtcttatctt ccgcttcagt aacaccgtcg
      961 gtaagtagga tgagcaaatc gccggccgct agcgaccatt gctgctcgat gttcacgtgg
     1021 tcgatatcgt ccgtgatcgc gaggaacgtg cccggcgtgc gaagcatctc ggtcttctgc
     1081 gtggccgcgc gccagcagac cgcatccatg tgggcgccgg cgacgacgag cgtgccgtta
     1141 cggtcgtatc gcaatagcga cagcgtcatg tgacgctcgg cctccaaacg ttcctggacg
     1201 ttctcgaaca ggacccggtt gagcgtcttc agcacgaagc tcggcttcgc catcggctcg
     1261 gcgcggacca gcgtcgcgac gcctgtctgc accatcatca tcacgagacc cgacgtgaga
     1321 ccgtgacccg acgcatcgcc gaccgcgatc cagcagccag tgggaatcgg cagcacgtcg
     1381 tagtagtcgc cgccgacctc gcttgccgtc atcatgcgtg ccgcgatctc gaggccgtcg
     1441 acgttcatgt tgcgcggcaa gatcgaggtc tggatgcgcg ccgcgatctc gagctcgcgt
     1501 gcgagtcgct ccttgtcggc gagcgccatg cgcgagtcgt cgatctcctt tgccatctga
     1561 ttcatcgcgg tgccgagcgc ctgcacctcg gtcgggccgc caacggatcg cggcgacagg
     1621 tcaccttgcg cgattgcctg cgtgcgcgtg acgagcgcgt cgatcggccg cgatagccgg
     1681 cgcgcgagac cgagcgcgag cacgagcgtc gcgagcgcag cacccgcgag caagagcagc
     1741 agaatgcgga tgatcttgcg ccccggtgcg agtgcttcgt cgatcgagtg gagcagaaga
     1801 tattccaccc gactctcgcc gtgatagccg ggcacggcga cgagctgcgc gtaccagtgc
     1861 gtgccaccga gctcgacctc gcgctcgccg ccgcgaatcg cgacgaggcc cgtgcgcagc
     1921 tcttcggcgc tgatcgcttc gggccggctc gtcaatgcag cgtgatcgtc gacgaccgtc
     1981 agcttgccgc cggtttgccg cgcgaccgtg gcggcaaagt catcatcgat cgcatggccg
     2041 acgacgagcg caccgacgac gcgcgcgccg aactcgagcc ggcagccttg cacttgaaac
     2101 gccttgttgt cggcgagcca cacgccgctc tcctcgcctt ttgcgagcgc gttcgcgacg
     2161 accggctgat gcgtcatgtc gtcgccggcc gcttgcgggt cggcgttgtc ggcgatcaga
     2221 cggccatcgg cgtcgacgat cacgaacaca ccggagtgca acgtctcggc gagcgtgcgt
     2281 accgcgtcga ggatcgtgtc gcgcgcgacg tcttcggtcg cgacgacggc cttgaggcgc
     2341 ggctcctcgg cgacgacgcg gcactcctgg cgtaacagcg atgcacgcga ggagtgttgg
     2401 tccagcgtta cgacgcggct gcgcgcgagg tcgttcgcga cgcgcgtacg cgtggagcga
     2461 tcgaacacgt acgcgatggt tccgatcgtt gcgccgagca ctgcgacgac gagcagcagc
     2521 acccagagga atacagtcgt gcgcaacttc acggcgtcgg acccttcttt gccactcgtt
     2581 tgcccggcgg tggcggcttc acgctcaccg tcttcggcag cgcgccgccg agccggatct
     2641 tcgcgctcgc catcgcctgc tcttcgatct cggtgatgcc gagctccttg gcgatcgagt
     2701 ctgcgatccc gcgatacgtc acgtcgacct cgagcgtgcc gatgtttgca gcggcgagct
     2761 tgaacgtgtc ctgccagctg ccgccgacgg caatccgcgt gtcagtaccg accttcgtcg
     2821 cgcgccagaa cggcacttcg gcgccgctcg catcgacgag cacgcggccg agcgcgcgcg
     2881 tatcagccgc gacctgcgcc cccgctgcgt cgagcacgcg tgcacggacg atgatgcgtc
     2941 gctccggcaa accggcgggc accgcatgac cggcgttgtt gttgcgaacc gtcaccgcaa
     3001 cggcgagcga gccggcgtcg tctttgacgg tgacatcgag cccgagtgcg cgcttccgta
     3061 gatcctgggc gagcccgagc agcccgtgat gcggcacgcc gctacgcacc gacgagccga
     3121 ccgcgagcgc ggcgcgctcc tttggcatgt ggcagtcctg gcactgcatg cccttcgcgg
     3181 cttcggggcc ggccttccag tccgcgtatt cggtgaacac cggaatgccc ttgggctccc
     3241 accaatgaca ggtcccgcag atcgcggcct gctggtgctc cttcgagcag cccatgcgat
     3301 ggaagtagtg atccttgaga tcgcagcgcg gcccgtactt caccatgtcg tcgatcgcga
     3361 gccggaactt gccgccgtcc ggcgacggct gtggctcgcg caacgtgtgg cagacgtcgc
     3421 aggtcacgcc ttcgctcacg gtgatgtcgc gcggaacgac cttcgcgagc ggcgcgtggc
     3481 agcgatcgca cgacgggtct ttcgacgccg cgacggacac cttgtagaac gacgagctca
     3541 ccgccttcgc gtgcgctgac acttcccact cgtcgaacat cttctcgtgg cactcaccgc
     3601 atcgaccggc accggtgacg aacaccttgg actcggccgc gtcggcaacg acagccaccg
     3661 ccgcgtcgct gatcggcgcg acgggcgcag catcccgctt cgccggtggc gccttcttcc
     3721 ccgacggctc acacgctgcc agcagcagga cgattacttg taacgcccgt atggcgtgcc
     3781 gtcctttcgc gtgtgcgttt ccttcttcgc gacctcgctc atctcgagct tcacttccgc
     3841 cgcctcgcct gccttgaccg tgaccgtgcc cttcgcttcg tcgcccgtcg gtagccacgc
     3901 gacgaccgga tattcgccgg ccggcacgtt gtcgatgcgg aacgcgccgt ccgcgcccgt
     3961 gatcgtgtag taggcgttat cgagcacctt caccttcgcg atcatctcgg ggtgaatgtt
     4021 gcagtacacg tcgacggtgc cggagcgctt catctcgacg gacttcgcgg tgccgctctt
     4081 gtagaggccg aggtcgaagc gcgcgggacg cgagaccgag aacacgttgt gaaaaatctt
     4141 gtcctcgttc gggaagtcga tcgtggtgcc cttcacgacg atcgtcagcg gcggctcgaa
     4201 ctgcttctcg cgctggcgaa tgatcgcgtg gtccttcggc accggcggcg ttccggccac
     4261 gccttcgagg tacacgacga cgcccttctt gtcggacttc gccttgccct tggcgttgat
     4321 ggtcaccgtg ccacgcacgc tgccggtcga cggcttgctg cgtgcatctc cgacgaagac
     4381 cacgagagct gcgaacatca cgatgatggg tagtcgtcgc atcgtgcctc ctagtacttg
     4441 atcaccagcg agctggtgag aacgtcgttg ggaatctgcg gcacgtcttt ctcgagctcg
     4501 cggttcttcg tgccttcgat cttcacgatc acgcgcgtgc cgatttccgc acgcagcccg
     4561 agcgtcgcgc gcgcgaggtc tgcgatgtag acgaagctcg cgcccgagta gtgatacgcg
     4621 tcgcgccagt ccacgcggat gtacggcacg aacatgttcg tcacgcggta ggcgacgagg
     4681 ccataggcac ccttgtagtt gaggcacggc gcgacgtcgc actcggccat cgccatcgga
     4741 gaggtcttgc ccttcgccct gccatgtacg tactcggcgg tgacctcgag gtccttccag
     4801 tcgatgtgca tgtcggcacc gtagtgccac tggtacacgt cgttgaacgg ttggaagtcc
     4861 tgcgcgcccc acgcgccgga cacgccgaac tcgacgacct tcgcgatctg atacgacagg
     4921 cggccggcgc cggtcttcat ctgattcgtg tcggtctcgc tcgagaacgg aaacccttca
     4981 ctgaagtggc tgccgttcgt gaccgcgacg ttgaggatga gcgcctcgtc gagaaaccgc
     5041 gcacgcgcct tgaggccggt cggccgcccg caggtgtagc gacagatcaa cgacggcgtg
     5101 acgccgacgc gatccggcga ttcgagcgag cggtactcgt agccgacaac cgagtcgaac
     5161 ttgccggctt gcagcatcag gtcgaacttc tcgatgcgcg gccgccactc ggcgtacgcg
     5221 agcttgacgt cgatgtagtc gccaacgaac aggcccgcgg gatcggagac gtcacgcgca
     5281 cgcggcacga agtcgatgct cgcgttcagc tgcgcggtct cgccgatgcc ggagaacagc
     5341 gccaggttga cggcgttgac gagcatcgtc gacttgccgt gcgagttgat cggatcgaac
     5401 gtgaccgcac gcgactcgcc ggtatccgcg acgtcgccac gcgaattgat cgcggtagac
     5461 agtggatcgc cgtagaacac ccaggagccg gggaccttgt cgatgaactc gggaaaccag
     5521 gtctgcagta ggtcggttcg cgtgccggcg ccgttgccgc tcgtcacgaa cgcacccatg
     5581 tcgacgtagc cgctgatctt gccggtcagc ggcagcagcg actgcagctt ctcctcgaga
     5641 tacgagttgt cgtccttcgc ctgctcgagc tcgtcttcga ctttctgcag ccgctgttcg
     5701 agctcgtgca acttcagatc gaccttcggc tcggcgggct ccttcggctc cgtctgcgtt
     5761 ggcggctcgg gctgcgcggg ctgtgccggc tgcggttggg ggtcggccgg ttgcgcccac
     5821 gcaggcgtgg caacgatcaa cgaagcaagg aagatgcgtt tcacagggat tgaatccatg
     5881 agcgaatgag cgcgatgccg gtgtcgtccg tcttctcggt ggcgatcggt ggcatctgca
     5941 cgttcatcgt acgctgcgtg atgcgaaaga acgcggcgct catgtcggga tcgcctgcaa
     6001 caatccgatg cacgtagccg tggccaaccc actgctcgag cgggacgccg acggtggtct
     6061 gcacgatctg agtcgtcgcc gcgtcgtgct cgtccacgtc tagccgcaga atcatgtgtg
     6121 agtcggccca cgcgctgcca ttggggttgt gacagtggcc gcagttcgcg tgcaggtaac
     6181 cgagcgctgg attcggcgcc tcgtaggtcg tgcccgccgg tggtggcgac gacagcggca
     6241 acgccgagac atcaccgagc tgcagagccg acatgccgag cgagtgaccg ggctcgccga
     6301 tgtggcaacg ccagcacatg tccgccggtg gcgcatcgtg ctcggtgcca cgcaggttct
     6361 gggcgccatc cttcgcgaac tcggcgtcgg attcgttgtc atcccacacg taggagccgg
     6421 cgagcgtgtc gcgctcgagg tcgccggtat ctgccacccg ccagatcaag cgagtctcta
     6481 gacgtttgcc gtcgagcgaa aattctttga agaacttcgt gccgaccggg aacttccagt
     6541 ggttcatgtc ggtggtgtcg atcgtcgcgc cggtcggcag ctggtaccag cgatgcttct
     6601 cggcagcgtc cgaccacagc acgttgttcg gcgtgaactc gtgcaggcgc ggcgacagcg
     6661 tcttcgtccg aatgttcgtg tagagcccgg tctgcgacaa ccgatcgggc aaaacggtag
     6721 gaagcacctc ggcatcaggc ggcacgaccg gtgggtccga acaggcaacc accgcgagga
     6781 gcaagaacca cggacagcgc actacaacac tcagggcacc ggaggacctt ggcagttacc
     6841 gcacgggatg tagcaaccgt cgtcgggaac ctgatcgccg tcgtcgtcgg tgaagttgcc
     6901 gcactgcgcg tcggtctcgg gcccagcggc gatcggtggc ggtccgcagt tcgcaagggc
     6961 gccatcgtta tcgatcgccg ggatggtgta gaggtactcg acgatgtcgg cgagatcctg
     7021 cggttcgagc gctgcgtaac ccgagacgag accaccgtgc gttgccgcgc agacctgctt
     7081 gccgtcggtg tctttgccct tcgcgatcgc gtcggagatt tgctgcttcg tccagccgcc
     7141 cacgcccttc ggaaacgcct ccggcgtgat gttacgcgtc gcgatcgcgg gcgggaatgc
     7201 aacgtccttg aggccgagct gctcttgcgt gaacacgcga ccgccgccga agaacttcga
     7261 cttgtcgaac tcgaggaagc tgcccgggag ggtcggcgag tggcagtcga tgcagaggcc
     7321 tacctgtgtc gagaggtaac ggccacgcat cgcgctctcg ttgttcgcgc cgccggccgg
     7381 catcggaatc tcggaatcg
//
LOCUS       DASUFX010000291         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1432927, whole genome shotgun sequence.
ACCESSION   DASUFX010000291 DASUFX010000000
VERSION     DASUFX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432927"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VFV99_11495"
     CDS             <1..1269
                     /locus_tag="VFV99_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059978.1"
                     /translation="RTGTGRYLRVTERRTPEMGTVTVVADVTEDVHHEAELTTAREIA
                     EAASAAKSEFLSSMSHELRTPLNAILGFAQLLERDRKQPLSERQLERLSHVLRGGEHL
                     LRLIDDILDLSRIEAGRISISSEPVAVREVLQEVVHTLEPMATRAQIQIAEPIVPTDA
                     ARVVADRTRLAQILMNFGSNAIKYGKQGGHVTFEVTQRRDALRITVIDDGIGIPEDKR
                     DKVFEPFQRAGQETGPIEGTGIGLTISKRLAELMRGAVGFDSEVGRGSRFWIDIPVHR
                     AAAGEDEAGAVDAAARSPLARDEVRHKIVYVEDNPSNIAFMREVMDELPGVELLTAPT
                     AEIGLPLIRAHKPSAVIMDINLPGMSGFDAVRQLREWGDTKDIPVIGLSAAALARDTA
                     RAKEAGFFRYLTKPVKVDELMKTLEELLGH"
     gene            complement(1266..2558)
                     /locus_tag="VFV99_11500"
     CDS             complement(1266..2558)
                     /locus_tag="VFV99_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5059979.1"
                     /translation="MTAVERHAAKVARIVAQLKAHRGDKPLSLRKKAVAHQVPKAKDL
                     RYRDDKIDISDLTEILEVDPVKQICVAESGVTFVDLVRATLAHGLVPIVVPELKTITI
                     GGAVAGCSIESMSFVHGGFHDTCLEYEVIAATGDVLTCTPDNQHKLVFQMVHGGFGTL
                     GILAKLVFKLVPAKRYVHVVYEKHTTIESYQASIARHTETRDVEFMDGIIHSPSLYVL
                     SVGHFVDRAPYTNRYDRFKVYYKSTATRDEDYLETPQYFFRYDRGVTNVRLPLLSSTH
                     LLKIAANLHWLLRSEKPTITLDVFVPFSKVPEFLTWYEHEFGFFPLWCVPYRRVHDYE
                     WLADDYWRGLKDGMFLDLAIYGMKQKGDKNYHRIMEEKLRELGGIKTLIAHNYYPEAE
                     FWTIFNKRNYDAVKQITDPKHLFRDLYTKTCKAVMGRA"
     gene            2639..3199
                     /locus_tag="VFV99_11505"
     CDS             2639..3199
                     /locus_tag="VFV99_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5059980.1"
                     /translation="MTGGPGAGKTALLEVAQRELCRHVVVLPEAASILWRGQFPRRTS
                     LAARQAAQRTIARVQLELQRMTLEEGHAAVILCDRGTLDGLAYWPGAPENYFAALGST
                     REQELARYQAVIHMRTPPAHNGYSHANPYRIESAEEAAKLDQLILAAWDGHPRRSVIE
                     TEPDFFVKLQRALIAIRDQLPACCVK"
     gene            3208..4257
                     /locus_tag="VFV99_11510"
     CDS             3208..4257
                     /locus_tag="VFV99_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059981.1"
                     /translation="MTDARATQAAELMEQFAVRTGLTDQAAPRRYLWTDAFALCNFIA
                     LHRATENPRYEALANKLVERVHHVLAPHHDVEHPTAAGLRIGKRLPERGPNDYFDDEL
                     EWDRDGQYFHYLTKWMHALDQLARATNRPRLNLWARELAATAHRAFTYSTHGVRRMYW
                     KMSIDLSRPLVSSMGHHDPLDGFVTTLVLQAHAQLPGPELDGALRDFSSMIERRALAT
                     ADPLGIGGLLTDALRMTSLVRRGVPGADPELLDAIVTAAVDGLQQIDRQQDLRAPASY
                     RLGFRELGLAIGLAAAEQIGDARLIPYLPLRSKIERFWLDANHRATSTWLEHADINDV
                     MLATSLLPDGFLGTA"
     gene            4278..>4714
                     /locus_tag="VFV99_11515"
     CDS             4278..>4714
                     /locus_tag="VFV99_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059982.1"
                     /translation="MAPYPVQLEVSSPARFDRMQLLVRLGIAIALGWLGITMGWLAWL
                     LFLVLPVIAAIVVSTKGAEYYLHTTATKLWPPLTWLLSFSAYMLLITDEVPIDETSLR
                     TELHVTGTPSISSALARIIMSIPSAIVLCLLGFASCVLWVIALG"
BASE COUNT          812 a         1534 c         1506 g          862 t
ORIGIN      
        1 cgaaccggta ccggccgata cttgcgcgtg acggagcgcc gcacgcccga gatggggacg
       61 gtgacggtcg tcgccgacgt caccgaagac gttcatcacg aagccgagct caccaccgca
      121 cgcgagatcg cagaggccgc gagcgctgca aagagcgagt tcttgtcgtc gatgagtcac
      181 gagctccgca caccgctcaa tgcgatcctc ggcttcgcgc agctgctcga gcgcgatcgc
      241 aagcaaccgc tgagcgagcg ccagctcgag cggctctcgc acgtgctgcg cggcggcgag
      301 cacttgttgc gtctgatcga cgacatcctc gatctctcac gcatcgaggc cggccggatc
      361 tcgatctcgt cggagccggt cgccgtgcgc gaggtgctgc aggaggtcgt ccacacgctc
      421 gagccgatgg ccacgcgtgc gcagatccag atcgccgagc cgatcgtgcc gaccgatgcg
      481 gcccgcgtcg ttgccgatcg cacccggctc gcacagatcc tgatgaactt cggctcgaac
      541 gcgatcaagt acggcaagca gggtggccac gtcacgttcg aggtcaccca acgtcgcgac
      601 gcgttgcgca tcaccgtgat cgacgacggc atcgggatcc ccgaggacaa gcgcgacaag
      661 gtgttcgagc cgttccagcg cgcgggccag gagaccggcc cgatcgaagg caccggcatc
      721 ggcctcacga tcagcaagcg cctcgccgag ctgatgaggg gcgccgtcgg cttcgacagt
      781 gaggtaggtc gcggctcgcg gttttggatc gacatcccgg ttcaccgcgc ggctgccggt
      841 gaggatgagg ccggcgctgt cgacgcggcg gcgcggtcgc cgctcgctcg cgacgaggtg
      901 cgccacaaga tcgtttacgt cgaggacaat ccgtcgaaca tcgcgttcat gcgtgaggtc
      961 atggacgagc tacccggcgt cgagctactc actgcgccga ccgcggagat cggtctgccg
     1021 ctgatccgcg cgcataaacc gagcgcggtg atcatggaca tcaatcttcc cggcatgagc
     1081 ggcttcgatg ccgtccgcca gctgcgcgag tggggcgaca ccaaggacat cccggtgatc
     1141 ggcctgtcgg ccgccgcgct cgcacgtgat accgcgcgcg cgaaggaagc cggcttcttc
     1201 cggtatctga cgaagccggt gaaggtcgac gagctgatga agacgctcga agagctgctc
     1261 ggacactagg cgcggcccat caccgctttg cacgtcttcg tgtagagatc gcggaacagg
     1321 tgcttcggat cggtgatctg tttcaccgcg tcgtagttgc gcttgttgaa gatcgtccag
     1381 aactccgcct ccggatagta gttgtgcgcg atcagcgtct tgatgccgcc gagctcgcgc
     1441 agcttctcct ccatgatgcg gtggtagttc ttgtcgccct tctgcttcat gccgtagatc
     1501 gcgaggtcga ggaacattcc gtccttcagg ccgcgccagt aatcgtcggc gagccactcg
     1561 tagtcgtgga cgcggcgata cggcacgcac cagagcggaa agaatccgaa ctcgtgctcg
     1621 taccaggtga ggaactcggg aaccttcgag aacggcacga acacgtcgag cgtgatcgtc
     1681 ggtttctccg atcgcagcag ccagtgcaga ttcgccgcga tcttcagcaa gtgcgtcgag
     1741 ctgagcagcg gtaaccgcac gttcgtgacg ccgcggtcgt agcggaagaa gtactgcggt
     1801 gtctcgaggt agtcctcgtc gcgcgtcgcg gtgctcttgt agtagacctt gaagcggtcg
     1861 tagcgattcg tgtacggcgc gcgatcgacg aaatgcccga cgctgagcac gtagagcgac
     1921 ggcgagtgga tgatgccgtc catgaactcg acatcgcggg tctcggtgtg gcgcgcgatc
     1981 gacgcctggt acgactcgat cgtcgtgtgc ttctcgtaga cgacgtgcac gtagcgcttc
     2041 gccggcacga gcttgaacac gagcttcgcg agaatgccga gcgttccgaa gccgccgtgg
     2101 accatctgga acacgagctt gtgctggttg tcgggcgtgc aggtcagcac gtcgccggtc
     2161 gctgcgatca cctcgtactc gagacacgtg tcgtgaaagc cgccatggac gaacgacatc
     2221 gattcgatcg agcagcccgc gaccgcgccg ccgatcgtga tcgtcttgag ctcgggaacg
     2281 acgatcggga cgaggccatg cgcgagcgtc gcgcggacga gatcgacgaa cgtgacgccc
     2341 gactcggcga cgcagatctg cttcaccgga tcgacctcga ggatctcggt cagatcgctg
     2401 atatcgatct tgtcgtcccg gtagcgcaga tccttcgctt tcgggacctg gtgcgcgacg
     2461 gccttcttgc ggagcgatag cggcttgtcg ccgcgatgtg ctttgagttg cgccacgatc
     2521 cgcgcgactt tggcggcatg gcgttcgaca gctgtcatgt gggcgacatc ttgcaccagc
     2581 acggtgcgtg cctccaccgt gtgcgtgcga tctggtccat atcccccggt tggttgtcgt
     2641 gaccggcggc ccgggtgcgg gcaagaccgc gctcctcgag gtcgcgcaac gcgagctgtg
     2701 ccggcacgtg gtcgtgttac ccgaagctgc cagcatcttg tggcgaggtc agtttccacg
     2761 ccgtacgtcg cttgctgctc ggcaggctgc acaacgcacg atcgcgcgcg tccagctcga
     2821 gctccaacgc atgacgctcg aagaaggcca cgccgctgtg atcctctgcg atcgcggcac
     2881 actcgatggt ctcgcgtatt ggcccggcgc ccccgagaac tacttcgcgg cgctcggttc
     2941 gacacgcgaa caggagctgg cgcgctatca ggccgtgatc cacatgcgga ctccacctgc
     3001 gcacaacggc tactcgcacg cgaacccata ccgcatcgag agcgccgaag aagccgcgaa
     3061 gctcgaccag ctgatcctcg ccgcgtggga tggccatccg cgccgctctg tgatcgagac
     3121 cgagccagac tttttcgtga agctgcagcg agcgctcatc gcgatccgcg atcagctgcc
     3181 ggcatgctgc gtgaagtaac gatcagcatg accgacgcac gagcgacgca agctgccgag
     3241 ctgatggagc agtttgcagt gcgcaccggg cttaccgatc aagccgcgcc acggcgctac
     3301 ctgtggaccg atgcatttgc gctctgcaac ttcatcgcac tgcaccgcgc aaccgagaac
     3361 ccgcgctacg aagcgctcgc aaacaagctc gtcgaacgcg ttcatcacgt gctcgctccg
     3421 catcacgatg tcgagcatcc gaccgcagcg ggcctgcgca tcggtaagcg gctgcccgag
     3481 cgtggcccca acgattactt cgacgacgag ctcgaatggg atcgcgacgg ccagtacttc
     3541 cactacctga cgaagtggat gcacgcgctg gatcagctcg cgcgcgcgac gaatcggccg
     3601 cggctcaact tgtgggcacg cgagctcgca gcgacggcgc atcgcgcgtt cacgtattcg
     3661 acgcacggcg tgcggcgcat gtactggaag atgagcatcg atctgtcgcg accactcgtg
     3721 tcttcgatgg gtcatcacga tccgctcgac ggcttcgtca cgacgctcgt gctgcaggcg
     3781 catgcacagc tccccgggcc cgagctggat ggagcgctgc gcgatttctc gtcgatgatc
     3841 gagcgccgcg cgctcgcgac cgccgatccg ctcggcatcg gcggcttgct caccgatgcg
     3901 ctgcgaatga cgagcctcgt ccggcgcggc gtgcccggcg cggatcccga gctcctcgac
     3961 gcgatcgtca ccgccgcggt cgacggactt cagcagatcg atcgccagca ggatttgcgt
     4021 gcgccggctt cgtaccggct aggttttcgc gagctcggct tggcgatcgg cctagctgcg
     4081 gccgagcaga tcggcgacgc gcgcctgatt ccgtatctac cgctccgcag caaaatcgaa
     4141 cgcttctggc tcgatgcaaa ccaccgcgcg acgagtacgt ggctcgagca cgcggacatc
     4201 aacgacgtca tgctcgcgac gagcctgctt ccggacggct ttctcggcac ggcatgacaa
     4261 atgctgctct gacgggtatg gcgccctacc ccgttcagct cgaagtctcg tcgccggcgc
     4321 gcttcgaccg catgcagctg ctcgtgcgcc tcggcatcgc aatcgcgctc ggctggctcg
     4381 gcatcacgat gggttggctc gcgtggctct tgttcctcgt gctgccggtg atcgccgcga
     4441 tcgtcgtgtc tacgaaaggt gctgagtact acctgcacac gaccgcgacg aagctctggc
     4501 cgccactgac gtggttgctc tcgttctcgg cctacatgct gctgatcacc gacgaggttc
     4561 cgatcgacga gacaagcctg cgtaccgagc tccacgtgac cggcacgccg tcgatcagct
     4621 ctgcgctggc tcgtatcatc atgtctatcc cgagcgcgat cgtcttgtgt ttgcttgggt
     4681 tcgcatcctg tgttctgtgg gtcatcgcgc tcgg
//
LOCUS       DASUFX010000292         6521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1850738, whole genome shotgun sequence.
ACCESSION   DASUFX010000292 DASUFX010000000
VERSION     DASUFX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850738"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VFV99_11520"
     CDS             complement(<1..1079)
                     /locus_tag="VFV99_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5059983.1"
                     /translation="MRRLQVLGALALVHVAAPVYADDEESPEQVVVVNAAAKLGSVGQ
                     IARLRRVLDSRNMLVKLDKNLEATLDGRNVLIADVDAIREAYGSADYELALKIIDTDE
                     QRILGEAVSRDPIPALAELSQWRGIIAAALNQQDEAERWFRAAYRFNPAWVIDKKLAS
                     PRVRSLVKRAKREPDETGTLRVNTDPNEAQISINGGEAHAAGEKLSLPVGKHLVMITA
                     PKRKPYAELVEIAQGETYRIDINLDPESTLDRAARLVDASVAAPAGKPRLKRAKALAK
                     LTGVPRLLFIEDGGEDQIMVRLYDVDTKKVSKPLELGNEASSAAIARQIIAALDPENL
                     VDVDTIIISQRGEQRPTPWYKRWYV"
     gene            complement(1058..2995)
                     /locus_tag="VFV99_11525"
     CDS             complement(1058..2995)
                     /locus_tag="VFV99_11525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5059984.1"
                     /translation="MRPASEPPARRQGRYVLGELLGRGGMAEVFAGHVLGSHGFQKPV
                     AIKRLLPELANDHVFVERLISEAKLLVGMTHGNIVSVLDLARDGDDVFLVMEFVDGPS
                     LRQLLKARGARGLSLGNATYIVQAAAAGLEFAHARPGGAIIHADISPSNLLLTTSGEV
                     RVADFGIARREGGGAGIVEGKWAYMAPEQARGEALTPRSDVFALGVVLYELITGQHPF
                     GQRVTPDERDSGPIRIAPPRVVNPAVPAGLDAICMQALADKTRDRYARMQHFIDAIID
                     ERFANQWREGANDLAQAIREVSPGAVHTSAGPRTQVTDNPVTIITRSLISQPARLPSA
                     PIDGSVETQDTPSLSTGARTSMVEMPSVSGAQTSLIEVPSESAARTSAVLIPPSGALT
                     SMVEIPPPRTMPRSSSQMPFQLPAALLPSPELASVHGGSIVTGASVVDARSAHMSKWT
                     FAVLGIAALIGVIAGIAITLGPDREPAATRESPPAELVTHVVMPPEAPAPAAAPPTPE
                     PTVPEPTVPEPTVPAEAVAAEPPPAAVAESVAAPATKEASTAPRRARTAPPSKKVASG
                     AADATLRVNTYEGTWAWAYVNGQKQYAPGAKFKVAAGRYTVKLSNPDLGVVTCPVSIG
                     SGEVMTLKVELEERACDVSKY"
     gene            3194..4009
                     /locus_tag="VFV99_11530"
     CDS             3194..4009
                     /locus_tag="VFV99_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5059985.1"
                     /translation="MHKTPSPESQRIFAPGLFAGQVAIVTGGGSGIGLATALEFVRLG
                     GRVAICGRSEDKLAAAKTQLVEAGSADRVRTATCDIREPAQVEAFVKDTLAAWSRLDI
                     LVNNAGGQFPSPAQHISPNGFLAVVKNNLVGTFNMSREVANQWMIPHKGGRIVNVIAN
                     IYRGFPGMVHTGAARAGVENMTMTLAVEWAQFNILVNAVAPGIIISSGTAQYPPQLLE
                     RGIAETPLKRAGTVDEVAASIVFLASPGAQYITGTTLRIDGAQSLWGHTWEIP"
     gene            4009..4767
                     /locus_tag="VFV99_11535"
     CDS             4009..4767
                     /locus_tag="VFV99_11535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5059986.1"
                     /translation="MDLGLAGKACLVAGASRGIGRAIAHSLAREGARVAAVARGEADL
                     ATLMRELGEGHTSIVADVVTAEGASAAVDGTIAAFGGLDVVIAVVGKSFARDASGMDD
                     DDLAKSLDMNLWSSARVAQRAVPHLVARGGGSITLITSIWGREAGGAPGYNVAKAGVM
                     ALAKALARDYAKHNIRVNSIAPGSILFPGGGWDRRMKADPDAIADMVKRQLPFGRFGT
                     PEEVADVVAFVASPKASWIAGACVVVDGAQSRAF"
     gene            4767..5210
                     /locus_tag="VFV99_11540"
     CDS             4767..5210
                     /locus_tag="VFV99_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059987.1"
                     /translation="MRIVAFAGALLLVVACKKPIAEPREPERSENPPMHNVAPRDPEG
                     PVPEAQTPRPVRGRAVLAGDQRLNERLRSPDLLPLGSRIRRRLQVEAASRDELCACLD
                     HVLDAAGNPALMTIELRVTLAEHALGNYRIMMNMADERLRRESIR"
     gene            6027..>6521
                     /locus_tag="VFV99_11545"
     CDS             6027..>6521
                     /locus_tag="VFV99_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059988.1"
                     /translation="MSVHGRRRGGTGSQARRRDRSWYGVSVHRKLPDERPDAGKCESK
                     EAPDSSPGLINEGCGDDENSCSAGGSDRDGAPVRFSSRRVETNPITVFSVPTPDAIFF
                     GYRMMWGSHVSNIAAHKVGSDTGPTIHHSNEVTHFLGSGWVDDYSDRLFLNVRDKAVS
                     SPPTI"
BASE COUNT         1081 a         2106 c         2166 g         1168 t
ORIGIN      
        1 cagacgtacc agcgcttgta ccaaggtgtc ggtctctgct cgccgcgctg gctgatgatg
       61 atcgtgtcga catcgacgag gttttcggga tcgagagccg cgatgatctg gcgcgcgatc
      121 gctgccgatg acgcctcgtt gccaagctcg agcggcttgg agaccttctt ggtgtcgaca
      181 tcgtagaggc ggaccatgat ctggtcttcg ccgccgtctt cgataaacaa caggcgcggc
      241 acgcccgtca gcttcgcgag cgccttggcg cgcttcagcc ggggcttgcc ggctggtgcg
      301 gcgacgctgg catcgacgag gcgcgcagcg cgatcgagcg tcgactcggg atcgaggttg
      361 atgtcgatgc gatacgtctc accctgcgcg atctcgacga gctcggcgta cggcttgcgc
      421 ttgggcgcgg tgatcatcac gaggtgcttg ccgaccggca acgagagctt ctcgccagca
      481 gcgtgcgctt cgccgccatt gatcgagatc tgcgcttcgt tcgggtcggt gttgacgcgc
      541 agcgtgccgg tctcgtcggg ctcgcgcttc gcgcgcttga cgagcgaacg cacgcgcggc
      601 gacgcgagct tcttgtcgat cacccacgcg gggttgaagc gatacgcggc gcggaaccac
      661 cgctcggctt cgtcctgctg gttcagcgcg gcggcgatga tgccgcgcca ctggctcagc
      721 tccgcgagcg cgggaatcgg atcgcggctc accgcttcgc cgaggatgcg ctgctcgtcg
      781 gtgtcgatga tcttgagcgc gagctcgtaa tcagcgctgc cgtaggcctc tcggatcgcg
      841 tcgacgtcgg cgatcaatac gttgcggcca tcgagcgtcg cctcgagatt cttgtcgagc
      901 ttgacgagca tgttgcgcga gtcgaggacg cggcgcaggc gcgcgatctg gccgacgctg
      961 ccgagcttcg ctgctgcgtt gacgacgaca acctgctccg gtgactcctc gtcgtccgcg
     1021 tacacaggcg cggcgacgtg tacgagcgcc agcgcgccta gtacttggag acgtcgcatg
     1081 ccctctcctc tagctcgact ttgagcgtca tcacttcacc cgagccgatc gagaccgggc
     1141 aggtgacgac gccgagatcg gggttgctca gcttgacggt gtaacgaccg gccgcgacct
     1201 tgaacttcgc gccgggcgcg tactgtttct ggccgttgac gtatgcccac gcccacgtgc
     1261 cttcgtacgt gttcacacgc agcgttgcgt cggcggcgcc gcttgcgacc ttcttgctcg
     1321 gcggcgcggt tcgcgctctg cgcggcgcgg tcgaagcttc tttcgtcgcc ggtgcggcaa
     1381 ccgattcggc cacagcggcg ggcggtggct cggcagcgac ggcttcggcc ggcacggtcg
     1441 gttcgggcac cgtcggttcg ggcaccgtcg gttcgggcgt cggcggcgca gcggctggcg
     1501 caggcgcctc gggcggcatc acgacgtgcg tgacgagctc ggccggcgga ctctcgcgtg
     1561 tagcggctgg ctcgcgatcg ggaccgagcg tgatcgcgat tcctgcgatc acgccgatca
     1621 gtgcagcgat gccgagcacg gcgaacgtcc acttgctcat gtgcgcgctg cgtgcgtcga
     1681 cgaccgacgc gcccgtcacg atcgagccgc cgtgcacgga cgcgagctcc gggctcggca
     1741 acagtgcggc gggcagctga aacggcatct gcgacgagct gcgcggcatc gtccgtggcg
     1801 gcggaatctc gaccatcgag gtgagcgcgc ccgacggcgg aatcagcacg gcagacgtcc
     1861 gggcggcgct ctcgctcggg acttcgatca gcgacgtctg tgcaccgctc acgctcggca
     1921 tctcgaccat tgatgtgcgt gcgccggtcg acagcgacgg ggtgtcctgc gtctcgacgg
     1981 aaccgtcgat cggcgccgat ggcaaccgag ccggctggct gatcaacgag cgcgtgatga
     2041 tcgtgaccgg gttgtcggtc acctgcgtgc gcgggccggc ggacgtgtgc acggcacccg
     2101 gcgacacctc gcgaatcgcc tgggcgagat cattggcacc ttcgcgccac tggttcgcga
     2161 agcgctcgtc gatgatcgcg tcgatgaaat gctgcattcg cgcgtagcga tctcgcgtct
     2221 tgtcggccag tgcctgcatg cagatcgcat cgagtcctgc cggcacggct ggattgacga
     2281 cgcgcggcgg cgcgatccgg atcggtccgc tgtcgcgttc gtcgggcgtg acgcgctggc
     2341 caaacgggtg ctggccggtg atcagctcgt acaacacgac accgagcgcg aacacgtcgg
     2401 aacgcggcgt gagtgcttcg ccgcgtgctt gctcgggtgc catatacgcc cacttgcctt
     2461 cgacgatgcc agcgccgccg ccttcgcgac gcgcgatgcc gaaatcagca acccggactt
     2521 cgcccgacgt cgtcagcaac aagttgctgg gcgagatatc ggcgtgaatg atcgcgccgc
     2581 ccggccgcgc gtgcgcgaac tcgaggcccg cggcggcggc ttgcacgatg tacgtcgcat
     2641 tgccgagcga caatccgcgt gctccgcgtg ccttcagcag ttgccgcaag ctcgggccat
     2701 cgacgaactc catgacgagg aacacgtcgt cgccgtcgcg cgcgagatcg agaacgctca
     2761 cgatgttgcc gtgagtcatg ccgaccagga gcttcgcttc gctgatcagg cgttcgacga
     2821 acacgtgatc gttcgccaac tccggaagca gtcgtttgat cgcgaccggc ttctggaagc
     2881 cgtgcgaccc gagtacgtgg cccgcgaaca cttcggccat accgccccgt ccgagcagct
     2941 cgccaaggac ataacgaccc tggcggcgtg ccggtggttc ggaggctgga cgcacgtctc
     3001 ctatggcggt ggatatcccg gccacggctt ctgaacacga gtgcattcag cgtgcccgag
     3061 ctaacagatc gtacattcga gtgtttcact ccgattaccg tcggagacac cccggggtgg
     3121 gggaggatct atggggagtg gtgcccctcc gtggggacgt aaccctcacg gttcgtttat
     3181 gttgcgcacc gtaatgcaca aaactccgtc gcccgaatcg cagcgcatct tcgctccagg
     3241 cctgttcgcc gggcaggtgg caatcgtcac cggtggtggc tcgggcatcg ggctcgccac
     3301 tgcgctcgaa ttcgtacgcc tcggcggccg ggtcgcgatc tgcggccgca gcgaggacaa
     3361 gctcgccgcc gccaagacac agctcgtcga agcgggcagt gccgatcgtg ttcgcaccgc
     3421 gacatgcgat atccgcgagc cggcgcaggt cgaagcgttc gtcaaagaca cgctcgccgc
     3481 atggagccgt ctcgacatcc tcgtcaacaa cgccggcggt cagttcccat cgcccgcgca
     3541 gcacatctcg ccaaacggtt ttctcgcggt cgtgaagaac aacctcgtcg gtacgttcaa
     3601 catgtctcgc gaagtcgcga atcagtggat gatcccgcac aagggcggtc gcatcgtgaa
     3661 cgtgatcgcg aacatctacc gcggctttcc gggcatggtg cacacgggcg cggcgcgcgc
     3721 tggtgtcgag aacatgacga tgacactcgc ggtcgagtgg gcgcagttca acatcctcgt
     3781 caacgcagtc gcgccgggca tcatcatctc gagcggcacc gcgcagtacc cgccgcaact
     3841 cctcgagcgt gggatcgcgg agacaccact caagcgcgcg ggcaccgtcg atgaagtcgc
     3901 tgcgtcgatc gtgtttctcg cgtcgccggg tgcgcagtac atcacgggca ccacgctgcg
     3961 catcgacggt gcgcaatcgc tctggggcca cacctgggag atcccgtaat ggacctcggg
     4021 ctcgccggca aagcatgcct cgtcgctgga gcatcgcgtg gcatcggtcg cgcgatcgca
     4081 cactcgctcg cgcgtgaagg tgcacgtgtc gccgcagtcg cgcgtggcga agccgatctc
     4141 gcgacgctga tgcgcgagct cggcgaagga cacacgtcga tcgtcgcgga cgtcgtgaca
     4201 gcggaaggtg cgagcgccgc ggtcgacggc acgatcgctg cgttcggtgg tctcgacgtc
     4261 gtgatcgcgg tcgtcggcaa atcgttcgcg cgcgatgcgt cgggcatgga cgatgacgat
     4321 ctcgcgaagt cgctcgatat gaacctgtgg tcgagtgcgc gcgtcgcgca gcgcgcggtg
     4381 ccgcacctgg tcgcgcgcgg cggcggatcg atcacgctca tcacgtcgat ctggggccgc
     4441 gaagccggcg gtgcgccggg ctacaacgtc gcgaaggcgg gcgtgatggc gctcgcaaaa
     4501 gcgctcgcgc gcgactacgc gaagcacaac attcgcgtga acagcatcgc gccgggatcg
     4561 atcctgttcc cgggtggcgg ctgggaccgc cgcatgaaag cggatcccga cgcgatcgcg
     4621 gacatggtca agcgccagct gccgttcggc cgcttcggca cgcccgaaga ggtggccgac
     4681 gtcgtcgcat tcgtcgcgtc gccgaaggcg tcgtggatcg caggcgcgtg cgtcgtcgtc
     4741 gacggcgcac agtcgagggc attctgatgc ggatcgtcgc attcgccgga gcactcctcc
     4801 tcgtcgtcgc ctgcaagaag ccaatcgccg agccgcgcga acccgagcga tcggagaacc
     4861 cgccgatgca caacgtggcg cctcgggatc ccgaaggccc ggttccggaa gcccagacgc
     4921 cgcgacctgt tcgtggtcgc gccgtgctcg ccggcgacca gcggctcaac gagcggctac
     4981 gcagccccga tctgcttccg ctcggcagcc ggatccggcg ccgcctccag gtcgaggctg
     5041 cgtcccgcga tgaactgtgt gcgtgcctcg atcacgtgct cgacgccgcc ggcaatccgg
     5101 cgctgatgac catcgagctg cgcgtcacgc tcgccgagca cgcgctcggc aactaccgga
     5161 tcatgatgaa tatggccgac gagcggttaa ggcgcgagtc gattcgctag cgattccggc
     5221 acgtaggtcc gccgcaaatt gttgcacctg tcggttgcaa gagatgcgct tgcaagcaac
     5281 acgcgcacgg tgtgagatct ccgtcggcag ggcactcatc tcgtgcgctg ttgggagccg
     5341 ttccaccatg aagtgctgtc tcatcattgc gctatgtttc tgcgcctgcg gcacctcgaa
     5401 gcccgagcaa tcgtcgagtc aacaggccgt tagtggctcg ggtggctcgg gctgcgacat
     5461 gacgccaatc gacgacaagg gaacgctgcc cgcgtgcgcg gccggaacaa gcacgacgag
     5521 tggtcccggt cgtccgtttg accgtccaac tccagagcct tccgtcccgc tctctgcaga
     5581 tgagaaggct gcagccgcaa gagccgacgc gctatccgcg gctcggaatc catctggtca
     5641 gtctccgctg gaccgtggtt tcggaggtcc gcaatgagcg aagcatgcac tatggtccgt
     5701 tggcggctcg ttcgacggcg gtgtccactg ttgctaggtg cgctcgcgat gctgctcgtg
     5761 ctctttattg cccccacgag ggcggaagcg acctgcggca cctatggcgt atatcattgg
     5821 cgtgaactga ccaacgaccc gaatctgtgc acagaccctc atggcgtcca gtgcatccaa
     5881 ggtaactcct tttgcaatca atgcagtttt ggctgcgagt ggccaggctg tgtctgtaac
     5941 tgcggcacat tggtctttat cgataccgac aagtacagct gcgcgacggg ggcatacgtc
     6001 tcgaccgaca caaatatctt ctcgacgtgt ccgtgcacgg caggcggcgc ggcggaactg
     6061 gcagccaagc ccgacggcgc gatcgttcct ggtacggggt atcagtgcac agaaaactgc
     6121 cagacgagcg gcccgacgcc ggcaagtgcg agtcgaagga agctccagac agttcgccag
     6181 gtttgatcaa tgaaggctgc ggcgatgacg aaaacagctg ctcggcagga ggctccgatc
     6241 gagacggcgc accggtgaga ttctcgtcgc gacgagtgga aacaaatccg atcacggtct
     6301 tcagcgttcc aaccccggac gcgatctttt tcggttaccg aatgatgtgg ggctcgcacg
     6361 tgtcgaacat tgccgcacac aaagtcggct ccgacacggg accgacgatt caccattcga
     6421 acgaggtgac tcactttctc gggagcggct gggtcgatga ctactcggat cgcctgttct
     6481 taaacgtgcg ggacaaggcg gtatcgagtc ccccgacgat a
//
LOCUS       DASUFX010000293         7355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1712737, whole genome shotgun sequence.
ACCESSION   DASUFX010000293 DASUFX010000000
VERSION     DASUFX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712737"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..866
                     /locus_tag="VFV99_11550"
     CDS             78..866
                     /locus_tag="VFV99_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5059989.1"
                     /translation="MSPSAGRYTALVIHGDGDVLDTLTQWLEANGFDVVAAPSGFRAQ
                     AHLEGDRPVEVVIAPWDDTHPVGGEIYHWVLKNRPDLRSRFVFIANDVPPEFDAVVGG
                     RCLAVPLAAIEEVIRVASGIVKRVRTPPRGVPIMFRPGRPGLLLVEDDPLLLTAMADL
                     FSEQGYSVSQVDSLKNALELVEFKDFDVIVADWKMHDGSAATLYKWITKNKPHLAARI
                     VFLSEADLDDSGPIAPGRPMFRKGQDSQALSETLRQIVAQVRGS"
     gene            complement(863..3646)
                     /gene="lon"
                     /locus_tag="VFV99_11555"
     CDS             complement(863..3646)
                     /gene="lon"
                     /locus_tag="VFV99_11555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5059990.1"
                     /translation="MASELLPLMELPILPLRTMVLFPHAALPMTLNRAAASQAVAAAG
                     EDGLVAVVTQAHPEAELPTGSDLFAIGTGAVVRQLTQPATDTAMVIVEGLERIAIVDI
                     VQTHPYLRARVRRLASERPVIDSVYAAARSNLLDSFQEMVQLSIVLPDELGVVARSIT
                     DDSALTDMIGSALVETAPATRQDILATLNIRRRMDKLNEVIAREVEALKIREKLRVEV
                     QTKIADSQKEIVLREQLKAIRKELGEERGEENALADLRTKIEEGGLPSEALKEATREL
                     DRLQDMATGSPEYSMVRNYCEWMAELPWSTSTASPIDLIRAKQILDEDHFDLDKVKER
                     ILEFLAVQQRRHTQRGPLICLVGPPGVGKTSIGRSIARATTRQFVRISLGGTNDEAEI
                     RGHRRTYIGSLPGQIIRGLRRAGSCDPVFMLDEIDKLSRDFRGDPAAALLEVLDPEQN
                     SAFRDHYLDVPFDLSRVLFVATANILDTIPEPLRDRMELIDLPGYIDEDKLQIAKRYL
                     VPKQVVDNGLVIGEEIEFTDDAILEIIRGHTHEAGVRKLEQLIASVSRKRARQLAEGA
                     NGKLIVNRGVIGGMLGTAKFRVESQVHERTKRPGVSIALAWTASGGEVLFVECSKLGH
                     GHGNVTLTGQMGDVMQESARTAVSWVRAHADRYGIAEDMFEKHDIHIHVPAGAVPKDG
                     PSAGIVMVVSLVSLLTNRPVKPYTALTGEITLSGVLLPIGGLKEKVLAARRSKLRTII
                     VPMENDPNLREEVPDHLRGEVEVKLASSIEQAVDFALSPEGGSLMAHQKIQDVMTKQV
                     HCVHEESSIRDVARLMRDQKIGDVLVTDAQGKLCGIITDRDIVVRAVADEGNDLDQLR
                     AKDICTTDRIVTLEPSDNVGDAIRLMTEKAVRRIPVIENELPVGIVTIGDLAAQHDPG
                     SALGQISQAAPNN"
     gene            complement(3713..5074)
                     /locus_tag="VFV99_11560"
     CDS             complement(3713..5074)
                     /locus_tag="VFV99_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5059991.1"
                     /translation="MARAAKRPAKAGAKKSAKKSPAARAAAKKPVPKAKAAAKKPAKA
                     AKRAKPVKAKSRAKQAARVKPAPRAETSVPPVTQPMRAESSSDAIPRMVGPEPSDSGR
                     RSLSARERVLRSSPVVEILDDPHPIGALRRFLEGVRHEATPQQAQIALGAAQLMLLPI
                     ARDHRGGGEVKEIIDLVLSHWDQFGDRRKGYHAQEFLKNAFIAIGVDRDRIQRLQDLV
                     PASPSSDLLFSVAGAHAVARDKVAMLRAVERALEAGATAAQFRRDADFVVYTNDPDFS
                     VLLARAEVPQIPVDVEPHIPTVRAALDQLVATLKEFGEPIELRPPVRLDAILDAERAR
                     KISLPNDYRALLTITNGMTLWEHMFFGAGDYRELTQLALSAQRYLQSSAEAGERGIEE
                     CVPLAKWGTPTDWLLYDPRGRLRGGEPGYLLRVDNQPQPIEDLSTVLERIEYMARETL
                     GTN"
     gene            complement(5108..7090)
                     /locus_tag="VFV99_11565"
     CDS             complement(5108..7090)
                     /locus_tag="VFV99_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5059992.1"
                     /translation="MAERSFALTPGKRVLYLTKDPERIRQQLAGTLTLRMSDVSADEL
                     LDDINTDAMTPAWVCFDFDPNEIAKNAYAGLIVAGQRLIPTGALANGGFEVIVAGAQK
                     GVGSSREQAAQCEVFSGSQLAIASSFAPIHARNNINIGQLMGDHAMIRRLEAGESIPL
                     SEFTRGQDPITVLIIESGGLLPFCARLAKHEVVVPAPGTQARPMNLAEKILAAKLLPG
                     QGSHVKPGDAVLVKVDAGYSHEFTTAQVDKFLTDEYGDYKLQNPTKFAVFEDHLIYAT
                     GVASMAKFADKIERLRELQKAFAAKTGVRNYSATGGVSPGICHQVAREQFIDPGDFVQ
                     ATDSHTCMGGASGALAWGVGATEYAALLYWGYTPIAVPESIRFELTGRLPNGTSAKDV
                     MLHILATYAKREQTLNRVMEFGGEGLFALSPDERATLANMATECSARGAVMEVDDTML
                     KWIAERRPGVSVDQLRTKVVTPDKGAHYDGGVHKIDLSAIQPMVATPGDPDRGIASDP
                     KNGALVAEVGHQKIDIAYGGSCTAGKRDDIDMYARVMADAERSGKRVADGVKFYIQFG
                     SQEVEQYAQKQGYTDLFKRTGVEVIKPGCGACIGCGPGVSDRGDQVTVSAINRNYKGR
                     SGPGKLFLASPLTVAASAVTGEIVEYEDGMFSPPKK"
BASE COUNT         1197 a         2442 c         2333 g         1383 t
ORIGIN      
        1 gaaagtccgt cgcatatgtc gaagcctagg atgcgaatgc catcaaatca aacgcttcac
       61 tgctatgatc cgacgcgatg tcgccgtctg ccggtcgcta caccgccctg gttatccatg
      121 gcgacggcga tgtgctggat acgttgaccc aatggctcga ggccaacgga tttgacgtgg
      181 ttgcggcgcc gtcggggttc cgcgcacagg cgcacctcga gggcgaccgg ccggtcgagg
      241 tcgtgattgc gccctgggac gacacccatc ccgtcggcgg cgagatttac cactgggtcc
      301 tcaagaatcg ccccgatttg cggtcgcggt tcgtgttcat cgcgaacgac gtgccaccgg
      361 aattcgacgc cgtcgtgggc ggtaggtgtc tcgccgtacc gcttgccgcg atcgaagagg
      421 tcattcgcgt cgccagcggc atcgtgaagc gtgtacgtac tcccccgcgc ggcgtgccga
      481 tcatgttccg cccgggccgg ccgggattac tgctcgtcga ggacgatccg ctgctgctca
      541 cggcgatggc ggatctgttc agcgagcaag gctattcggt gagccaggtc gacagcctca
      601 agaacgcgct cgagctggtc gagttcaagg acttcgacgt gatcgtcgcc gattggaaga
      661 tgcacgacgg cagcgcggcg acgctctaca agtggatcac caagaacaag ccgcacctcg
      721 cggcccgcat cgtgttcctc tcggaggccg atctcgacga ctccggaccg atcgcgcccg
      781 gccgtccgat gttccgcaag ggtcaggact cgcaggcgct gagcgagacg ctcaggcaga
      841 tcgtcgccca ggtccgcggt tcctagttgt tcggtgctgc ctggctgatc tggccgagcg
      901 ccgatcccgg atcgtgctgt gcggcgagat cgccgatcgt cacgataccg accggtagct
      961 cgttctcgat caccggaatg cggcgcaccg ccttttcggt catgagacgg atcgcatcgc
     1021 cgacgttatc gctcggttcg agggtgacga tgcgatcggt cgtacaaata tccttcgcgc
     1081 gcagctgatc gaggtcgttg ccctcgtcgg caaccgcgcg cacgacaatg tcacggtccg
     1141 tgatgatgcc gcacagtttg ccctgcgcgt ccgtgacgag tacgtcgccg atcttctgat
     1201 cgcgcatgag tcgcgcgacg tcgcgaatcg atgactcctc gtggacgcag tggacctgct
     1261 tggtcatcac gtcctgaatc ttctggtgtg ccataagtga acctccctcc ggggacaatg
     1321 caaaatccac ggcctgctcg atgctcgagg cgagcttgac ctcgacctct ccacgcaggt
     1381 gatcgggaac ttcctcgcga aggttcggat cgttttccat cggcacgatg atcgtgcgga
     1441 gcttgctgcg tctcgccgcc aggactttct ctttgaggcc gccgatcggc agcagcacac
     1501 cggagagcgt gatctcgccg gtcagcgcgg tgtacggctt gaccggccgg ttcgtcagta
     1561 gcgacacgag cgacaccacc atcacgatgc cggcgctcgg gccgtctttg ggcaccgcgc
     1621 ccgcgggaac gtgaatgtgg atgtcgtgct tctcgaacat gtcctcggcg atgccatagc
     1681 gatcggcgtg cgcacgcacc cagctcaccg ccgttcgcgc cgactcctgc atcacgtcgc
     1741 ccatctgacc ggtcaacgtg acgttgccgt ggccatggcc gagcttgcta cattcgacga
     1801 acagcacctc gccaccgctc gctgtccacg cgagcgcgat cgagacgccg ggtcgcttcg
     1861 tgcgttcgtg gacctgcgac tcgacacgga atttcgcggt gccgagcatg ccgccgatga
     1921 cgccgcggtt gacgatcaac ttgccgttcg cgccctctgc gagttggcgg gcacgcttgc
     1981 gcgacaccga tgcgatcagc tgctcgagct tgcgcacgcc ggcttcgtgc gtgtggccgc
     2041 ggatgatttc gagaatcgca tcgtcggtga actcgatctc ctcgccgatc acgagcccgt
     2101 tgtcgacgac ttgcttcggc accaggtaac gcttggcgat ctgaagcttg tcctcgtcga
     2161 tgtagcccgg cagatcgatc aattccatgc ggtcgcgcag cggctcgggg atcgtgtcga
     2221 ggatgttcgc cgtcgcgacg aacagcacgc gcgatagatc gaacggcacg tcgaggtagt
     2281 gatcgcggaa cgcgctgttc tgctcgggat cgagcacctc gagtaacgcc gccgccgggt
     2341 cgccgcggaa atcgcggctc agcttgtcga tctcgtcgag catgaacacc gggtcacagc
     2401 tgccggcgcg ccggaggcca cggatgatct ggccgggcag cgacccgatg tacgtgcggc
     2461 ggtggccgcg gatctcggcc tcgtcgttcg taccgccgag cgagatgcgt acgaactggc
     2521 gcgtggtcgc gcgggcgatc gaccggccga tcgacgtctt gccgacgccg ggcgggccga
     2581 cgaggcagat cagcggaccg cgttgcgtgt gccggcgctg ctgcacggcg aggaactcga
     2641 ggatgcgctc cttgaccttg tcgagatcga agtggtcctc gtcgaggatc tgcttcgcgc
     2701 ggatgagatc gatcggcgat gcggtgctcg tgctccacgg cagctccgcc atccactcgc
     2761 agtagttgcg caccatcgag tactcgggtg aaccggtcgc catgtcctgc aagcgatcga
     2821 gctcgcgcgt cgcttccttg agcgcttcgc tcggcagccc gccttcttcg atcttcgtgc
     2881 gcaggtccgc gagcgcgttc tcttcgcctc tctcctcgcc aagctccttg cggatcgctt
     2941 tgagctgctc gcgcagcacg atctcctttt gcgagtcggc gatcttcgtc tgcacttcga
     3001 cgcgcagctt ctcgcggatc ttcagcgcct cgacctcgcg cgcgatcacc tcgttgagct
     3061 tgtccatgcg ccgacggata ttgagcgtcg ccagaatgtc ctgccgggtg gcgggcgcgg
     3121 tctcgacgag cgccgagccg atcatgtcgg tcagcgcaga atcatccgtg atgctgcgtg
     3181 cgacgacgcc gagctcgtcg ggcaacacga tcgagagctg caccatctcc tggaacgagt
     3241 cgagcaagtt cgaacgcgca gcggcgtaga ccgaatcgat caccgggcgt tcgctcgcga
     3301 gcctgcgcac gcgtgcacgt aagtacggat gcgtctgcac gatgtcgacg atcgcaatgc
     3361 gctcgaggcc ttccacgatc accatcgcgg tgtcggtggc gggctgtgtc agctggcgta
     3421 ccactgcgcc cgtaccgatc gcgaacagat cgctgccggt gggcaattcg gcctccgggt
     3481 gcgcttgcgt cactaccgcg accagaccgt cctcacctgc cgcggcaacc gcttggctgg
     3541 ccgccgcacg gttgagcgtc atcggtagag ccgcgtgggg aaacaggacc atcgtccgca
     3601 gcggcaagat gggcagctcc atcagtggca acagctcgga cgccatatcc gagctttgga
     3661 gcaagcactg ttccttagac gtgggcgctc gccgcgcctg tgcgcgctcg ggttaattcg
     3721 tgcccaaggt ttcgcgcgcc atgtattcga tgcgctcgag caccgtcgag agatcttcga
     3781 tcggctgcgg ctggttgtcg acccgcagca ggtagccggg ctcgccgccg cgcaagcggc
     3841 cgcgcgggtc gtagagcagc caatcggtcg gcgtgcccca cttcgcgagt gggacgcatt
     3901 cctcgatgcc gcgctcgccg gcctccgccg agctctggag ataacgctgg gccgacagtg
     3961 cgagctgggt gagctcgcga tagtcgccgg cgccgaagaa catgtgttcc cacagcgtca
     4021 tgccgttcgt gatcgtaagt agcgcacggt agtcgtttgg cagggagatc tttcgcgcac
     4081 gctcggcatc gagaatcgca tcgaggcgca cgggtgggcg cagctcgatc ggctcgccaa
     4141 actctttgag tgtcgcgaca agttggtcga gcgcagcgcg caccgtcgga atatgcggtt
     4201 cgacgtcgac gggaatctgc ggcacctccg cacgtgcgag gagtaccgaa aaatcggggt
     4261 cgttcgtgta aaccacgaaa tcggcgtcgc gccgaaactg tgcggcggta gcgcccgctt
     4321 cgagcgcacg ctcgacggcg cgcaacatcg caactttgtc gcgtgcgact gcgtgtgcac
     4381 ccgcgacgct gaacagtaga tccgagctcg gacttgcggg caccagatcc tgcaacctct
     4441 gaatgcgatc gcgatcgaca ccgatcgcga tgaatgcgtt cttcaggaac tcctgcgcgt
     4501 gatagccctt gcgacggtcg ccgaactgat cccagtggct gagcacgaga tcgatgatct
     4561 ctttgacctc gccgccgccg cgatggtcgc gggcaatcgg cagcagcatc agctgcgcgg
     4621 caccgagtgc gatctgcgcc tgctgcggcg tggcttcgtg ccggacacct tcgaggaacc
     4681 gtcgcagcgc gccgatcggg tgtggatcat cgaggatctc gacgaccggc gacgaccgca
     4741 gcacgcgctc gcgcgcggac agcgaccggc gacccgaatc gcttggctcc ggaccgacca
     4801 tccgcggaat cgcatctgac gacgactcgg cccgcatcgg ctgcgtgacc ggcggtaccg
     4861 acgtctcggc gcgtggtgct ggtttcacgc gcgcagcctg cttcgcgcgc gatttcgcct
     4921 ttacgggctt cgcgcgcttt gcggctttcg cgggcttctt tgccgcggcc ttcgctttcg
     4981 ggaccggctt ctttgcggca gcgcgcgcag ccggcgactt cttcgccgac ttcttcgccc
     5041 cagccttggc aggcctcttc gccgctcgcg ccactgcgaa caacgtacca aaccctcgca
     5101 gcgccaacta cttcttgggc ggggagaaca tcccgtcttc gtactcgacg atctcgcccg
     5161 taacagccga cgccgcaacc gttagcggcg atgcaaggaa cagcttgcct ggtcccgaac
     5221 ggcccttgta gttgcgattg atcgccgaca ccgtcacctg atcaccgcga tcggagacac
     5281 cgggaccaca gccgatgcac gcgccgcagc ccggtttgat gacctcgaca ccggtgcgct
     5341 tgaacagatc ggtgtagccc tgcttctgcg cgtactgctc gacttcctgc gagccgaact
     5401 ggatgtagaa cttcacgccg tccgcgacgc gcttgccaga gcgctctgca tccgccatca
     5461 cgcgcgcgta catgtcgatg tcgtcgcgct tgccggcggt gcacgaaccg ccgtacgcga
     5521 tatcgatctt ctggtgaccg acctcggcga cgagcgcacc attctttgga tcgctcgcga
     5581 tgccgcgatc cggatcgccc ggcgtcgcga ccatcggctg gatcgccgac aggtcgatct
     5641 tgtggacgcc accgtcgtag tgcgctccct tgtccggcgt cacgaccttc gtacgcagct
     5701 ggtcgacgct cacgcctggt cggcgctcgg cgatccactt cagcatcgtg tcatcgactt
     5761 ccatcaccgc gccacgcgcc gagcactctg tcgccatgtt cgcgagcgtg gcgcgctcgt
     5821 ccggagacag cgcgaacagg ccctcgccac cgaattccat cacgcgattg agcgtctgct
     5881 cgcgcttcgc gtacgtcgcg aggatgtgga gcatcacgtc cttcgcgctc gtgccattcg
     5941 gcagccgacc ggtgagctcg aagcgaatcg actccggcac cgcgatcggc gtgtagcccc
     6001 agtagagcaa cgccgcgtac tcggtcgccc cgacgcccca tgcgagcgcg cccgacgcgc
     6061 cacccatgca cgtgtgcgag tcggtcgcct gcacgaagtc gcccggatcg atgaactgct
     6121 cgcgcgcgac ctgatggcag atgcccggtg acacgccgcc ggtggcgctg tagttgcgca
     6181 cgccggtctt cgccgcgaac gccttctgca gctcgcgcag ccgctcgatc ttgtcggcga
     6241 acttcgccat cgacgcgacg ccggtcgcgt agatcaagtg gtcctcgaag acggcaaact
     6301 tcgtcgggtt ctgcagcttg tagtcgccgt actcgtcggt gaggaacttg tcgacctgcg
     6361 cggtcgtgaa ctcgtgcgag tagccggcgt cgaccttgac gagcaccgcg tcgccgggct
     6421 tcacgtgcga gccctggccg ggcaacagct tcgccgcgag gatcttctcg gcgaggttca
     6481 tcggccgcgc ctgagtgccc ggcgccggca ccacgacctc gtgcttggcg agtcgcgcac
     6541 agaatggcag caggccgccg ctctcgatga tgagcacggt gatcggatcc tggccgcgcg
     6601 tgaactccga cagcgggatc gattcacccg cctcgagccg gcgaatcatc gcgtggtcgc
     6661 ccatcaactg accgatgttg atgttgttgc gtgcgtggat cggcgcgaac gagctcgcga
     6721 tcgcgagctg gctccctgaa aacacttcgc actgcgccgc ttgctcacga ctcgaaccga
     6781 cgcctttctg tgcgccggcc acgatcacct cgaagcctcc gttcgcgagc gcgccggtcg
     6841 gaatcaagcg ctggcccgcg acgatcaatc ctgcgtacgc attcttcgcg atctcgttcg
     6901 gatcgaaatc gaagcaaacc caggccgggg tcatcgcgtc ggtgttgatg tcgtcgagca
     6961 gctcgtctgc cgagacgtcg ctcatgcgca acgtgagcgt gccggcaagc tgctggcgga
     7021 tgcgctccgg atccttcgtg agatagagga cgcgcttacc aggagtgagc gcgaaactgc
     7081 gttccgccat cgcggggacc gtaccactct cgcgcgcgtg cgggagagtc tgagagaaaa
     7141 gcgaataccc cgccgacacg agagagcgaa atgacgaggt gcgcaataag cgcagtcacc
     7201 gccgcgggtt cgacctgtcc cttgcgtggt cccggtgctg tcccttcaac gtctcgacgc
     7261 agagcggtat gaccgagctc atgacgcggt ctccctatat cggccttcac gtcgctgcgc
     7321 ccaatcggcg tgccaccgcg gccgacatcg tcgcg
//
LOCUS       DASUFX010000294         9767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_599907,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000294 DASUFX010000000
VERSION     DASUFX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599907"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFV99_11570"
     CDS             complement(<1..721)
                     /locus_tag="VFV99_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5059993.1"
                     /translation="MDSAIDVRDLRKTYRTPFRRKQVEALRGVTFTVERGQIFGFVGP
                     NGAGKTTTIRTLMGLLKPTGGSATILGHAIPSRAARFKLGFLPESPYFYDYLTVGELL
                     DLAGRLFGLPHGLRKKRADELIEKVGLGRARAQSLKKYSKGMLQRAGLAQALMNDPEL
                     VVLDEPMSGLDPIGRKEVRDLIIELREAGKTVFFSSHILTDIEAIADRVAIVARGQLQ
                     SQGTPAELIQRTILGIDVQLRI"
     gene            complement(761..1390)
                     /locus_tag="VFV99_11575"
     CDS             complement(761..1390)
                     /locus_tag="VFV99_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5059994.1"
                     /translation="MDLRAPNPPAPAPEGGPQVAQAEAFKKKDSQKKVIKELRQPDEV
                     KATEDKPPTTATDGGGSGSATGPGTGSGDPTSTGTCTENCGPPEKKPEVIEDDPCKLN
                     PHSCDPIILPPPLIRGLRISGETQIQPPDIEKTALLRSGNSRATATAKVCIDERGNPT
                     TLSLFKASGYDGWDAKIIGAMRNWRYKPYKVGTRSVTVCGMVTFVYEIH"
     gene            1607..2866
                     /locus_tag="VFV99_11580"
     CDS             1607..2866
                     /locus_tag="VFV99_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5059995.1"
                     /translation="MTGALVVFCVTYLVIASRQLHFLKLDRPGGAVVGAVAMVIVGGL
                     PMDLALKAIDLHVLLLLFGVLVIAAYLTEARFFRRVAYLVLTRARSARTLLFGLVFVS
                     GALSAFLVNDTVCLVLTPLVLAVVVEARLPALPYLLALASAANVGGVISFSGNPQNMI
                     VGAAAHGTIGFAQYLALTLPVGIACLAANAALIAWLFRYDLPKGTLVDRHPPKPAINK
                     PLAIKGLAALTLFAGLALAGFTLAGAAMCAAAALMVAARSGTREVFARVDWTLLAFFG
                     GLFVVVAGVAHAGVLQDVFDAVAPVVARGDMTGDLAFIALVVIASNVVSNVPLVLVAV
                     AWVPHMPEPTWGYIMLAVASTLAGNLTLFGSIANIIVMETAGPRGEISFWRFLRYGSV
                     ITIVNLALAFAILAGERALGIPAKLGL"
     gene            complement(2873..3880)
                     /locus_tag="VFV99_11585"
     CDS             complement(2873..3880)
                     /locus_tag="VFV99_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5059996.1"
                     /translation="MVVWATACTDPIDPLAPRTFTFGPYPLEPGVEITDDCVSISLHN
                     DEPIYVNQAELTTSAGFHHSNWFWVPETQFAGADGTWSCDERHYSEAAAGVFGAVVFA
                     QSTQALHEIQAFPPGAATKIPPHAKLVAGLHLLNASDEPLAPKIELTITPIAADAVTA
                     DLHGFAFQNQTIQLPPHAKSRFTIECDLTKGLGLGGAETTRPPPDFRIYHVLAHYHEL
                     GSGLTFEALRDVDGGGETMFETQTRIGDALGSVLDPPFDLTGHSKIRFACEYDNPRDA
                     TVGWGVGDQEMCIVFAFTDSPLVWSGGAPYAESPGPGVDNNGVIEFTHACVTTSTDGT
                     H"
     gene            4011..4457
                     /locus_tag="VFV99_11590"
     CDS             4011..4457
                     /locus_tag="VFV99_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5059997.1"
                     /translation="MDKHAIDRIVETAVYLQAESRRLAKEQCAKHGITATQLNALKLL
                     QSVGDLSLSDLSKKMSATNSAITGLVDRMVEAGLVAREQSVEDRRVWKIRLTSEGRAM
                     AKKLDVAPWEILQRALMALPGEELGQLVVTLLKVVDHIESQTKENR"
     gene            4454..5311
                     /locus_tag="VFV99_11595"
     CDS             4454..5311
                     /locus_tag="VFV99_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5059998.1"
                     /translation="MSDKVIISCALTGAVTSKQLCPAIPYTPVEIAEEAQRAYDAGAA
                     IVHIHARTDQGLPSWDPATFKAIHEETKKRCPVLINWSTGGAGPMKDRVEHLKTRPHI
                     AALNMGSMNYAKWRRDKHNFAFNFVFTNSFDDIVGFAKAIREQGIKPEMECFDTGHVY
                     SHTVLEDLGLLDRPYHFSFIMGVLGGIPATARHLAFQASNIPAGSRWKVIGISRDQWP
                     LCMAALSLGGDIRVGLEDNFYLPTGDMARSNGDLVAAAVDMVKLSGRSVASVADARAM
                     LWPDGGGNF"
     gene            5320..6870
                     /locus_tag="VFV99_11600"
     CDS             5320..6870
                     /locus_tag="VFV99_11600"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5059999.1"
                     /translation="MRERVAELEQRRAKVREMGGAEKVAKHHDRGKLTARERLALLFD
                     DGAFTEVGIHATEMGPGAQTVPADAVVCGWGKVDGRFVAAAAYDFTVKGGSIGFSGET
                     KVTRMRELALRCRMPMVWLIDSAGARIDPKPNSDPDQISLFAGSGHLFREQVVMSGVV
                     PQVAAMVGPGAAGTAYIPGLADFVPMVAGKSSMALGGPPLVKAMTNQDVTEQDLGGSR
                     VHCEVSGCGDVEVESDEQCIAMTKRYLSFMPSHCGDKPPIESCDDPIDRADDALLDLV
                     PDNPKKTYDMYEVIRRLVDRGQYLDMKPRWARPMITCLARFGGRPAGIVANNPKQAGG
                     ILDADAADKAAHFIQICDAFNIPLVFLMDVPGFWVGKDHEHAGIIRHGAKMLHAMASA
                     TVPKLTVVTRKGYGAGYYVMAGRAFEPDLMVAWPGAEISVMGAEGMLGIAGSKLFKDG
                     EPPAEVKKQLAAMIQKNIDIYKVAGWGLVDDIIDPRETRALLCRAIEMSWNRVVERPA
                     RKHGIMPV"
     gene            6879..8090
                     /locus_tag="VFV99_11605"
     CDS             6879..8090
                     /locus_tag="VFV99_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5060000.1"
                     /translation="MFHFSQEHEMVRGIVRKWAESKLAPKVDALEAGEPPYELMRDLA
                     KTFGIAAMVRGAFEKIEQRAREGSTDSVKVSAMGDPALSAVLSIELSRISPGFTLAFG
                     ATMGLAGGAIVSRGTLEQKKRWGLPILTFEKIGGWGMTEPGAGSDAFRSMRTTAVPKD
                     GGYVLNGQKTFITNAPYADIIVVYAKIDRSDGTPLADRPIQPFIVERGDKGMETSKPM
                     RKMGMHSSPTGEVFLQDCFVPADRLLGGQEQAVGRASGRDVFHSERTGMAPMCVGIIE
                     KCLEISIDYAKQRVTWGKPIAEYQLVQDKLARMFVHLENCKNMMFKQLTLAAEGKTMT
                     PAEASAAKLYCARAATESAMEAVQLLGGNGYMQEYRVEMLARDAKLLQIGGGTDEIQI
                     INIARNLLATA"
     gene            complement(8095..9096)
                     /locus_tag="VFV99_11610"
     CDS             complement(8095..9096)
                     /locus_tag="VFV99_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5060001.1"
                     /translation="MRLYTYSGSGNGYKIELLLSLLGMQYDRIEVPIFDGASRTPEFL
                     KMNPAGRVPVVQLDDGTYLPESNAILWHLARDTKFMPHATLAQDRALGWLMFEQSEIE
                     PVIGSARFWIQTGRARTRRDETERRLEWAKQSLALLDRELADRAFLLGDEPSVADIAV
                     YAYTHVAPEIDLALPPNVAAWCKRIAALPGFVPAKLAYDPKAFVADAATKVAAYERYH
                     TQMDNKYGYSTTIDIPREVAEHEPWFNQTLTQVNDSLVRLGILGGEYHWHKHDNEDEC
                     FIVLDGELMLDIEGKGTVTLGPKLGYTVPKGVVHRTRANVKTTVLMIEAATVTPTGD"
     gene            complement(9180..9653)
                     /gene="sixA"
                     /locus_tag="VFV99_11615"
     CDS             complement(9180..9653)
                     /gene="sixA"
                     /locus_tag="VFV99_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX5060002.1"
                     /translation="MDVFLIRHAAAIDETLELRDPMRHLTAAGREQAKGLGDRLRWHD
                     CTPTHIWTSPLVRAVQTAELVATGLASATAVEVVPALAPGGESERSVVTALQALATNA
                     CVLVVGHEPGISSIGALLVGATDFPPLDKAEAVRISDGALRWRFAWNADAPSTSR"
BASE COUNT         1609 a         3100 c         3348 g         1710 t
ORIGIN      
        1 cgatgcgcag ctggacgtcg atgccgagga tcgtgcgttg gatcagctcg gcgggggtgc
       61 cctgcgattg cagctgcccg cgtgcgacga tcgcgacgcg atccgcgatc gcttcgatgt
      121 cggtgaggat gtgcgacgag aagaacacgg tcttgccggc ttcgcgcagc tcgatgatca
      181 ggtcgcgcac ctccttgcgg ccgatcggat cgagaccact catcggctcg tcgagcacga
      241 cgagctcggg atcgttcatc agcgcttgcg cgagccctgc gcgttgcagc atgcccttgc
      301 tgtatttctt gaggctctgc gcgcgcgcgc gaccgaggcc tactttctcg atcagctcgt
      361 ccgcgcgctt cttgcgcagt ccgtgcggca ggccgaacag ccggccggca agatcgagca
      421 gctcgccaac ggtcaggtag tcgtagaagt acggcgactc gggcagaaag ccgagcttga
      481 atcgcgcggc gcggctcggg atcgcgtggc cgaggatcgt tgccgatccg ccggtcggct
      541 tgagcaggcc catcagtgtg cggatcgtcg tcgtcttgcc ggcgccgttc gggccaacga
      601 agccgaagat ctggccgcgc tcgacggtga acgtcacgcc gcgcagcgct tcgacctgct
      661 tgcgtcgaaa cggggtgcgg taagtcttgc gtagatcgcg tacgtcgatc gcagagtcca
      721 agacccgaga actttatcag gcctcgggcc ggtcggcgct ctagtggatc tcgtagacga
      781 acgtcaccat gccgcagacc gtgaccgagc gggttccgac cttgtacggc ttgtagcgcc
      841 agttccgcat ggcaccgatg atcttcgcgt cccagccgtc atagcccgag gctttgaaca
      901 gcgagagcgt ggtcgggttg ccgcgttcgt cgatgcagac ctttgcggtc gcggttgccc
      961 gtgaattgcc cgaacggagc agcgcggtct tctcgatgtc gggcggctgg atctgggttt
     1021 cgccggagat gcgcaggccg cggatcagcg gcggcggcag gatgatcgga tcgcacgagt
     1081 gcggattcag cttgcagggg tcgtcctcga tcacctccgg cttcttctcg ggcgggccgc
     1141 agttctcggt gcaggtaccg gtgctggtcg gatcgccgct gcccgtgcct ggaccggttg
     1201 ccgagccgct gccgcctccg tcggtcgcgg tggtcggcgg cttgtcttcg gtcgccttga
     1261 cctcgtcggg ctggcgcagc tccttgatca ccttcttctg cgagtccttc ttcttgaacg
     1321 cctcggcctg ggcgacctgc gggccgcctt cgggcgcggg agcgggcgga ttcggcgcgc
     1381 ggagatccac cgtgtgccgg gggctatcga ggcgatcgag ccgccagaac cctgcgacaa
     1441 acagggccaa aacgacggcg gcgtggacga cgagggaggc tgcgacgagg gatcgacggg
     1501 ggcgcatttc cacggcaacg cagaggcctt ggcggggatc tatgccaggt gctgaccacc
     1561 cgcgacccag cgcctcgtag gtgtcgcagg tgtatcgtgc gtccgcgtga ctggcgcgct
     1621 cgttgtcttt tgcgtgacct atctggtgat cgcaagtcgc cagcttcact tcttgaagct
     1681 cgaccggccc ggcggcgcgg tggtcggcgc agtcgcgatg gtgatcgtcg gtggcctgcc
     1741 gatggatctc gcgctgaagg cgatcgatct gcacgtgctg ttgctgttgt tcggcgtgct
     1801 cgtgatcgcc gcgtacctca ccgaggcgcg gttcttccgg cgcgtcgcgt acctcgtgct
     1861 cacgcgcgca cgctcggcgc gcacgcttct gttcgggctc gtgttcgtct ccggcgcgct
     1921 gtccgcattc ctcgtcaacg acaccgtgtg cctcgtgctc acgccgctcg tcctcgctgt
     1981 cgtggtcgaa gcgcgattgc cggcattgcc gtacctgctc gcacttgcat ccgcggcgaa
     2041 cgtcggcggt gtcatcagtt tctccggtaa tccgcagaac atgatcgtcg gcgcggcggc
     2101 gcacgggacg atcgggttcg cgcagtacct cgcgctgacg ctgccggtcg gcatcgcgtg
     2161 cctggccgcg aacgccgcac tgatcgcgtg gctgttccgc tacgacttgc cgaagggcac
     2221 gctcgtcgat cgccatccac caaagcccgc catcaacaag ccgctcgcga tcaagggcct
     2281 cgccgcgctc acgctgttcg ccgggctcgc acttgccggc ttcacgctcg ccggtgctgc
     2341 aatgtgcgca gcagcggccc tgatggtcgc cgcccgctcc ggcacccgcg aggtgttcgc
     2401 gcgcgtcgac tggacgctgc tcgcgttctt cggtggcctg ttcgtcgtcg tcgccggcgt
     2461 cgcgcatgcc ggcgtgctcc aggacgtgtt cgatgctgtc gcgcccgtcg tcgcgcgcgg
     2521 tgacatgacc ggcgacctcg cgttcatcgc gctcgtcgtg atcgcgtcga acgtcgtgtc
     2581 gaacgtgccg ctcgtgctcg tcgcggtcgc ctgggtcccg cacatgccgg agccgacgtg
     2641 gggctacatc atgctcgcgg tcgcgtcgac gctcgccggc aacctgacgc tgttcggcag
     2701 catcgcgaac atcatcgtga tggagaccgc cggcccgcgc ggcgagatca gcttctggcg
     2761 cttcctgcgt tacggttcgg tgatcacgat cgtgaacctc gcgctcgcgt tcgcgatcct
     2821 cgccggcgaa cgtgcgctcg gcattccggc caagctcggc ctctagtgac gatcagtgag
     2881 tgccatccgt cgaggtcgtc acgcacgcgt gcgtgaactc gatgacgccg ttgttgtcga
     2941 cacccgggcc gggcgactcg gcatatggcg cgccgcccga ccacacgagc ggtgagtccg
     3001 tgaacgcgaa cacgatgcac atctcctggt cgccgacgcc ccagccaacg gtcgcgtcgc
     3061 gcggattgtc gtactcgcac gcgaaccgga tcttcgagtg gccggtgaga tcgaacggcg
     3121 gatcgagcac ggacccgagc gcgtcgccga tcctcgtctg ggtctcgaac atcgtctcgc
     3181 cgccgccgtc gacgtcgcgc agcgcctcga acgtgaggcc gctgccgagc tcgtgatagt
     3241 gggcgagcac gtgatagatg cggaagtctg gcggcggccg cgtcgtttcc gcgccaccga
     3301 gcccgaggcc cttcgtcaga tcgcactcga tcgtgaagcg cgactttgcg tgtggcggca
     3361 gctggatcgt ctggttctgg aatgcgaagc cgtgcaggtc ggcggtgact gcatccgcgg
     3421 cgatcggcgt gatcgtcagc tcgatcttcg gcgcaagcgg ctcgtcgctc gcgttgagca
     3481 agtgcagccc ggcgacgagc tttgcgtgcg gcgggatctt ggtcgccgcg ccgggcggga
     3541 acgcttggat ctcgtggagc gcctgcgtcg actgcgcgaa cacgaccgcg ccgaacacgc
     3601 cagcagcggc ctcgctgtag tggcgctcgt cgcacgacca cgtgccgtcg gcgccggcga
     3661 attgcgtctc gggaacccaa aaccagttcg agtgatggaa cccggcgctc gtcgtcagct
     3721 cggcctgatt gacgtagatg ggctcgtcgt tgtggaggct gatgctgacg cagtcgtccg
     3781 tgatctcgac accgggctcg agcgggtagg gtccgaacgt aaacgtgcgt ggcgcgagag
     3841 gatcgatcgg atctgtgcac gccgtcgccc agaccaccac tgctgcacaa attgaaagtt
     3901 cgcgcttgtg aatcattgcg ggtattgata tttcactatg tgaaatgtcc gcgtcaagaa
     3961 cgaatgcagc tcgcaggcaa ccaccccggc cccgcgagga gccggtcgcg gtggacaagc
     4021 atgcgatcga ccgcatcgtc gagaccgcgg tctatctaca ggcggagtcg cgccggctcg
     4081 cgaaggagca atgtgccaag catggcatca ctgcgacgca gctgaacgcg ctcaagctat
     4141 tgcagagcgt cggtgatctg tcgctgtctg atctgtcgaa gaagatgtcg gcgacgaact
     4201 cggcgatcac cgggctcgtc gaccgcatgg tcgaggccgg cctcgtcgcg cgcgagcaga
     4261 gcgtcgagga tcgccgagtg tggaagatcc ggctgacgtc cgagggacgc gcgatggcga
     4321 agaagctcga cgtcgcgccg tgggagatcc tgcagcgcgc gctgatggcg ctgcccggcg
     4381 aggagctcgg ccagctcgtc gtcacgctac tgaaggtcgt cgaccacatc gagagtcaga
     4441 cgaaggagaa ccgatgagcg acaaggtgat catctcgtgc gcgctgacgg gtgcggtcac
     4501 gagcaagcag ttgtgcccgg cgattccgta cacaccggtc gagatcgccg aggaggcgca
     4561 gcgcgcgtac gacgctggcg cagcgatcgt ccacatccat gcgcgcaccg atcaaggcct
     4621 gccgtcgtgg gacccggcga cgttcaaggc gatccatgaa gagaccaaga agcgctgccc
     4681 ggtattgatc aactggtcga ccggcggcgc ggggcccatg aaggaccgcg tcgagcacct
     4741 gaagacgcgg ccgcacattg ccgcgctcaa catgggctcg atgaactacg cgaagtggcg
     4801 gcgcgacaag cacaacttcg cattcaactt cgtgttcacg aactcgttcg acgacatcgt
     4861 gggcttcgcg aaggcaatcc gcgagcaggg catcaagccc gagatggagt gcttcgatac
     4921 cggccacgtc tactcgcaca ccgtgctcga ggatctgggc ctgctcgaca ggccgtatca
     4981 cttctcgttc atcatgggcg tgctcggcgg cattccggcg actgcacgcc acctggcgtt
     5041 ccaggcctcg aacattcccg ccggctcgcg gtggaaggtg atcggcatca gccgcgacca
     5101 gtggccgctg tgcatggctg cgctgtcgct cggcggcgac atccgcgtcg gcctcgaaga
     5161 caacttctat ctaccgaccg gcgacatggc gcgcagcaac ggcgatctcg tcgctgctgc
     5221 cgtcgatatg gtgaagctgt cggggcgcag cgtcgcatcg gttgctgatg cgcgcgccat
     5281 gctgtggccc gatggcggag ggaacttctg atggcgaaca tgcgcgaacg agtcgcggag
     5341 ctcgaacagc gtcgcgcgaa ggtgcgcgag atgggcggcg ccgagaaggt ggcgaagcac
     5401 cacgaccgcg gcaagttgac cgcgcgcgaa cggctcgcgc tgctattcga cgacggcgcg
     5461 ttcaccgagg tcggaatcca cgcgaccgag atgggcccgg gcgcgcagac cgtaccggcc
     5521 gatgcggttg tctgcggttg gggcaaggtc gacggacggt tcgttgccgc ggctgcgtat
     5581 gacttcacgg tgaagggcgg ctcgatcggc ttttccggcg agacgaaggt gacgcgcatg
     5641 cgcgagctgg cgctgcgctg ccgaatgccg atggtgtggt tgatcgattc ggcaggcgca
     5701 cgcatcgatc cgaagccgaa cagcgatccg gaccagatct cgctattcgc cggcagcggc
     5761 cacctgtttc gcgagcaggt cgtgatgagc ggcgtggttc cgcaggtcgc cgcaatggtg
     5821 ggcccgggcg ccgctggcac ggcatacatc cctgggctcg ccgacttcgt gcccatggtt
     5881 gcgggcaaga gctcgatggc gctcggcgga ccgccgcttg tcaaagcgat gacgaaccag
     5941 gacgtgaccg agcaagacct cggcgggtcg cgcgtgcact gcgaggtgtc aggctgcggc
     6001 gatgtcgagg tcgaaagcga cgagcagtgc atcgcaatga ccaagcgcta cctgtcgttc
     6061 atgccgagtc actgcggtga caagccgccg atcgagagct gtgacgatcc gatcgatcgc
     6121 gcggacgacg cgttgctcga tctcgtcccg gataacccga agaagaccta cgacatgtac
     6181 gaggtgattc gtcgcctcgt cgatcgcggc cagtatctcg acatgaagcc gcgctgggcg
     6241 cgtccgatga tcacgtgcct cgcacgattc ggtggccggc ccgccggcat cgtcgcgaac
     6301 aacccgaagc aagcaggcgg catcctcgac gcagacgccg ccgacaaggc agcgcacttc
     6361 atccagatct gcgatgcgtt caacatcccg ctcgtgttcc tcatggacgt gcctggcttc
     6421 tgggtcggca aggatcacga gcacgccggc atcattcgcc acggcgcaaa gatgctgcac
     6481 gcgatggcgt cggcgacggt gccaaagctg accgtggtga cccgcaaggg ctacggcgct
     6541 ggctactacg taatggcagg gcgcgcgttc gagcccgatc tgatggtcgc gtggcccggc
     6601 gccgagatca gcgtgatggg cgccgaaggc atgctcggca tcgcaggtag caagctgttc
     6661 aaagacggcg aaccgcccgc cgaggtgaag aagcagctcg ccgcaatgat ccagaagaat
     6721 atcgacatct acaaggtcgc ggggtggggg ctcgtcgatg acatcatcga tccacgcgag
     6781 acgcgcgcgc tgctgtgccg tgcgatcgag atgagctgga accgtgtcgt cgaacggccc
     6841 gcacgcaaac acgggatcat gcccgtctag gagcaaccat gttccacttc agccaagagc
     6901 acgagatggt acgcggcatc gtccgcaagt gggccgagtc gaagctcgcg ccgaaagtcg
     6961 acgcactcga agcgggcgag ccgccctacg agctgatgcg cgatctcgcg aagacgttcg
     7021 ggatcgccgc gatggtgcgc ggcgcgttcg aaaagatcga acagcgcgcg agggagggca
     7081 gtaccgacag cgtcaaagtt tcggcgatgg gtgacccggc gctctcggcc gtgttgtcga
     7141 tcgagctgtc tcggatctca ccgggattca cgctcgcgtt cggtgcgacg atggggctcg
     7201 cgggcggcgc gatcgtgtcg cgtggcacgc tcgagcaaaa gaagcgctgg ggcctgccga
     7261 tcctcacgtt cgagaagatc ggcgggtggg gcatgaccga gcccggcgct ggctccgacg
     7321 cgttccgctc gatgcggacg acggccgtgc cgaaggacgg cggctacgtg ctgaacgggc
     7381 aaaagacgtt catcacgaac gcgccgtacg cggacatcat cgtcgtctac gcgaagatcg
     7441 atcgcagcga tggcacgcca ctcgccgacc ggccgatcca accatttatc gtcgagcgcg
     7501 gggataaggg gatggagacg tcgaagccga tgcgcaagat gggcatgcac agctcgccga
     7561 ccggtgaggt gttcctgcag gactgtttcg tgccggcgga tcgcctgctc ggaggccagg
     7621 aacaggcggt cggtcgcgcg agcggcagag acgtgttcca cagcgagcgc accggaatgg
     7681 caccgatgtg cgtcggtatc atcgagaagt gtctcgagat ctcgatcgac tatgcgaagc
     7741 agcgcgtgac ctggggcaag ccgatcgccg agtaccagct cgtgcaggac aagctcgccc
     7801 gcatgttcgt gcacctcgag aactgcaaga acatgatgtt caagcagctg acgctggccg
     7861 ctgaggggaa gacgatgact cccgccgagg cgagcgcagc gaagctgtac tgcgcgcgcg
     7921 cagcgaccga gtccgcgatg gaggcagtgc agttgctcgg cggcaacggt tacatgcagg
     7981 agtatcgcgt cgagatgctc gcgcgcgacg cgaagctact gcagatcggt ggcggcaccg
     8041 acgagatcca gatcataaac atcgcgcgca acttgctcgc gaccgcttga ttgttcaatc
     8101 gccggtcggc gtgaccgtcg cggcctcgat catcagcacg gtcgtcttca cgttcgcgcg
     8161 tgtgcggtgc acgacgccct tcggcaccgt gtagccgagc ttcgggccga gcgtgaccgt
     8221 gcccttgccc tcgatgtcga gcatcagctc accgtcgagc acgatgaagc actcgtcctc
     8281 gttgtcgtgc ttgtgccagt gatactcgcc gccgagaatg ccgagacgca cgaggctgtc
     8341 gttgacctgc gtcagcgtct ggttgaacca cggctcgtgc tcggcgacct cgcgcgggat
     8401 gtcgatcgtc gtcgagtagc cgtacttgtt gtccatctgc gtgtggtagc gctcgtacgc
     8461 ggcgaccttc gttgcagcgt cggcaacgaa cgccttcgga tcgtacgcga gcttcgcggg
     8521 tacgaagccg gggagggcgg cgatccgctt gcaccacgcg gcgacgttcg gcggcagtgc
     8581 gagatcgatc tccggcgcga cgtgggtgta cgcgtagacg gcgatgtcgg cgaccgacgg
     8641 ctcgtcgccg agcaagaacg cgcgatcggc cagctcgcga tcgagcagcg cgagactctg
     8701 tttggcccac tcgagtcgcc gctcggtctc gtcgcgccgc gtgcgcgcgc ggccggtctg
     8761 gatccagaac cgtgcgctgc cgatcaccgg ctcgatctcc gactgctcga acatcaacca
     8821 gccgagcgcg cggtcctgtg cgagcgtcgc gtgcggcatg aatttcgtgt cgcgcgcgag
     8881 gtgccacagg atcgcgttcg attccggcag gtacgtgccg tcatcgagct ggaccacggg
     8941 cacgcggccg gccgggttca tcttcaggaa ctccggtgtg cgcgacgcgc cgtcgaagat
     9001 cggcacttcg attcggtcgt actgcatgcc gagcagcgag agcagaagct cgatcttgta
     9061 gccgttgccg gagcccgaat acgtgtagag gcgcatggcg ggagactagc ggtgatcgta
     9121 tgtccgcgct cgccggatcc ggacggagct gcaacacagc caccgtgcgg cgacagcgat
     9181 cagcggctcg tcgacggcgc gtccgcgttc cacgcgaacc gccagcgcag cgcaccgtcg
     9241 gagatccgca cggcttctgc cttgtcgagc ggcgggaagt cggtcgcgcc gaccagcagc
     9301 gcaccgatgc tggagatgcc gggctcgtgg ccgacgacga ggacgcacgc gttcgtggcg
     9361 agcgcttgca gggcggtgac aacactgcgc tcggactcgc cccccggtgc aagcgcaggg
     9421 acgacttcga ccgccgttgc cgacgcgagc ccggtcgcga cgagctcggc ggtttgtacg
     9481 gcgcgcacga gcgggcttgt ccagatgtgt gtcggcgtgc agtcgtgcca gcgcagccga
     9541 tcgccgaggc cctttgcctg ttcgcggcct gctgcggtga gatggcgcat cggatcgcgc
     9601 agctcgagcg tctcgtcgat cgccgccgcg tgccggatca agaacacgtc cacacctgga
     9661 gcgtacccca ggaccgtgta cgctcgcgcg catgaagctc tacggtacga ccacgtctcc
     9721 gttcgtccgg cgcgtgcgag tcgtcgcagc cgaggttggc gagcctg
//
LOCUS       DASUFX010000295         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_45578,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000295 DASUFX010000000
VERSION     DASUFX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45578"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..1326)
                     /gene="folD"
                     /locus_tag="VFV99_11620"
     CDS             complement(475..1326)
                     /gene="folD"
                     /locus_tag="VFV99_11620"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5060003.1"
                     /translation="MSANLIDGKAVAAKLRGEVATAAAALTARGIQPTLAVVLVGEDP
                     ASAVYVRSKTKAAREAGVEPRDHKLPATTSQAELLALVHQLNADPNVDGILVQMPLPS
                     QIDSDVIIRAIDPAKDVDGLTATSLGYLAQGKPVFVPCTPKGCMRLLREANVDPAGMR
                     AVVLGRSLLVGKPISLLLQNANATVTMCHSRTRDLADEVRRADIVVAAIGKPEMVKGD
                     WIRDGAVVLDVGINRLPTGKLVGDVDFAAAAERARAITPVPGGVGPMTIACLLENTVE
                     AAKRRRG"
     gene            complement(1353..1760)
                     /locus_tag="VFV99_11625"
     CDS             complement(1353..1760)
                     /locus_tag="VFV99_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5060004.1"
                     /translation="MPIYEYKCKACNREFEYQQRMSDPDKTECEACGASALERLISRT
                     AFALKGGGWYKDLYSSSKPESKSSEGSAKSDSSSSTPSSSSSDSSSSSSGGSSSTGTS
                     SGSSGGSSGSGGGTSGGGTSGGSSSSKAAASAS"
     gene            complement(1770..>2820)
                     /locus_tag="VFV99_11630"
     CDS             complement(1770..>2820)
                     /locus_tag="VFV99_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX5060005.1"
                     /translation="MVPNFSAYPFDRLRTLSRRDAQVESTIARWIAGHHAARGERLAR
                     LVGGTAVRVDVVPATAFDPFAARCAVRVGGAAIDVRGGSLGVRAIAQRLLGGPAELSA
                     PRPLGVVEKSIFALVVATALEDLGVAGEVWPVLDEELATTDVKTRVGRPSKGPAQAAE
                     REPIVELAITMGGEAERGHDVVLAVQIQAPADLLLRVPPSRGWPSWFAMTVDCPIVLG
                     RCALDGRALQRLAVRSVVTLDRPTATAELDVLGGTIGLAIAKNAVVGEVATGYVPRDM
                     SLPDTAHVELTVALGTTQLSLRQLGDLAIGQIVQLGRPLAGPFELRAAGRVVGRGELV
                     DVDGELAVRIVSLGD"
BASE COUNT          459 a          989 c          918 g          454 t
ORIGIN      
        1 cgcgtgcgat tcgacgagac cggcggggtg gccacggtcg tgtacgagga tgatcggaat
       61 ctcgccagca agggcgtgga tgtgtacgcg cgacgcgtga cggctgccgg cgtcgtccag
      121 cccgcgttcg ccgtagcgac cacgacgaaa cacgagtaca agccacgtct gacgacgcgg
      181 acggggggct cgcaggaagt cgtgttcgcg acgctcgcga cgaacacagc gttcgactac
      241 gacgttattg gccagacagt gagcagcggc gcactgagcg ggcccgcata tacgatttca
      301 gctacaacga accggcgcga aatgacaccc gtcatcggat gccagagcgc gacgagctgt
      361 gtatgtccat accgttggta tgacccggac agcaccgata cgaattcaga ccgtgtcaag
      421 gcacgcgctg tgacgtaccc ctaactgctc caattgtgcg cgcctcgcgt cgtgctagcc
      481 acggcggcgc ttcgccgctt cgaccgtgtt ctcgagcagg cacgcgatcg tcatcggtcc
      541 gacgccacct ggcaccggcg tgatcgcgcg cgcgcgttcg gcggcggctg cgaagtcgac
      601 atcgccgacg agcttgccgg tcggtagccg attgatgccg acgtcgagca cgaccgcgcc
      661 gtcgcggatc caatcgcctt tcaccatctc cggcttgccg atcgcagcga ccacgatatc
      721 ggcgcggcgt acctcgtccg cgaggtcgcg cgtgcgcgag tggcacatcg tgaccgttgc
      781 gttcgcgttc tgtagcagca gcgaaatcgg tttgccgacg agcaggctac ggccgagtac
      841 gaccgcgcgc atgcctgcgg gatcgacatt ggcctcgcgg agcaagcgca tgcagccctt
      901 cggcgtgcac ggtacgaaca ccggcttgcc ctgcgcgaga tagcccaagc tcgtcgcggt
      961 gaggccgtcg acatccttcg ccggatcgat cgcgcgaatg atcacgtcgc tgtcgatctg
     1021 cgatggcaac ggcatctgga cgagaatgcc gtcgacgttc ggatcggcat tgagctggtg
     1081 gacgagcgcg agcagctcgg cttgcgacgt cgttgccggc aacttgtgat cgcgcggctc
     1141 gacgccggct tcacgcgccg ccttcgtctt gctgcgcacg tacacggcag atgccggatc
     1201 ctcgccgacg agcacgaccg cgagcgtcgg ctggatgccg cgtgcggtca gcgccgcggc
     1261 ggcggttgca acttcaccgc gcagcttcgc tgcgactgct ttgccatcga tcaggttcgc
     1321 gctcacagtt gccgcgactc tacccgatcg agctacgaag cgctggccgc ggccttcgag
     1381 ctgctgctgc cgccactcgt gccgccgccg ctcgtgccgc cacccgatcc gctgctcccg
     1441 ccgctgctgc ccgagctggt accggtcgat gacgagccgc cagagctgct gctcgagcta
     1501 tcgctcgacg acgagctcgg tgtgctcgac gagctgtcgc tcttcgcgct tccctccgag
     1561 cttttcgact cgggcttgct cgacgagtag aggtccttgt accaaccgcc gcccttgagc
     1621 gcgaacgcgg tgcgcgagat cagtcgctcg agcgcgctgg cgccgcacgc ctcgcactcg
     1681 gtcttgtccg gatcactcat ccgctgctgg tattcgaact cgcgattgca ggctttgcac
     1741 ttgtactcgt agatcggcat ctacaactcc tagtcgccga ggctaacgat gcgcacggcg
     1801 agctcgccgt caacatctac caattctccg cggcccacga cacggccggc tgcgcgcagc
     1861 tcgaacggcc ccgcaagtgg gcggccgagc tgaacgatct gaccgatcgc cagatcgccg
     1921 agctggcgca gcgacagctg cgtggttccc agcgcgacgg tcagctcgac gtgagcggta
     1981 tcgggcagcg acatgtcgcg cggaacatac ccggtcgcga cctcccccac taccgcgttc
     2041 ttggcgatgg cgaggccgat cgtcccgccg agcacgtcga gctcagcggt cgccgtagga
     2101 cgatcgaggg tgacgacgct gcgaaccgcg agccgctgca gggcccgacc gtcgagcgcg
     2161 cagcggccga gcacgatcgg gcagtcgacc gtcatcgcaa accagctcgg ccagccgcgc
     2221 gacggcggca ctcgcagaag gaggtccgcc ggcgcttgga tctggacggc cagcacgacg
     2281 tcatggccgc gctcggcttc gccgcccatc gtgatcgcga gctcgacgat cggttcgcgc
     2341 tcggcggcct gcgcagggcc tttgctcggc cgaccgacgc gtgtcttcac atccgtcgtc
     2401 gcgagctcct catcgagcac cggccacact tcgccggcga cgccgagatc ttcgagcgcg
     2461 gtcgcgacca cgagcgcgaa gatcgatttc tcgacgacgc cgagcggtcg cggagcggaa
     2521 agctccgccg ggccaccgag cagccgctgc gcgatcgcgc gcacgccgag gcttccgccg
     2581 cgcacatcga tggccgcccc accgacccgc actgcgcagc gcgccgcaaa cggatcgaac
     2641 gcggtcgccg gtacgacgtc gacgcgcacc gcggttcccc cgaccagcct ggcgagccgc
     2701 tcgccgcgcg ctgcgtggtg ccccgcgatc cagcgcgcga tcgtcgactc gacctgcgcg
     2761 tcacgccggc tcagcgtgcg gaggcgatcg aacggatacg ccgaaaagtt gggcaccata
//
LOCUS       DASUFX010000296         6811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_600563,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000296 DASUFX010000000
VERSION     DASUFX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600563"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VFV99_11635"
     CDS             <1..1044
                     /locus_tag="VFV99_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5060006.1"
                     /translation="SRWWPQAERSDHGPFTRHGVRAVHFYNRGNDGDWIDLAYHSARD
                     VPARLHRDSLAETARLLRALVATSPPAHSGDGFWLPLARNTVVPRWTLIVGESVLILV
                     VLVALVLSREGLVAALARRSQRATTPPASGLLAGAGCYVLAIVFSTVFERSFASGHPA
                     PWLHEPLRALVGTMLVLLGALGLATRVAARIRPWCGGQRYRALAAITCTLIGAALLVV
                     GAAELAWVWLVPAAVIAVIPARAAVIAVLASALPIACVMNPWQLREAAWNGFLPLSLP
                     LSVLLGILAVPTIATLAWWLRRRTPSGPLGTLVLGMGCGLAVIIGLAFAATSRPACSP
                     AKFETFHLACERV"
     gene            1093..1635
                     /locus_tag="VFV99_11640"
     CDS             1093..1635
                     /locus_tag="VFV99_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060007.1"
                     /translation="MARIVVIFSLVLAACDVGELPGVGGADGGSGSGDGGGNGCIEVS
                     ATPAVGHHNPVMPTGCMSAAGCHSQAKGLGAAAPAYSYGGLLYKADKMTPYAGATVII
                     KLGTAEKKAAVADNGEFFLVPGIAGLDEPTATMTGSTEASACPSANLKMGGLLTAAGG
                     DCNSAACHQPGGVQGVIYAP"
     gene            complement(1636..2880)
                     /locus_tag="VFV99_11645"
     CDS             complement(1636..2880)
                     /locus_tag="VFV99_11645"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060008.1"
                     /translation="MRLGEMLVRDGRLTEPQLSQALAFQARDGGRLGTVLYEHGLVDL
                     EALTIYLGLELGIPIATGAMLERAKRAAVRLLQPTQAFKHKCVPLVVQDRQLIAAIED
                     PHDFATLEALTQITGYRVLPRVAPEVRVYYYIERYYGVARPARFMKFGDTPRGNDNVA
                     DAGLPAPPLPGLPPVPAAPVAAPGPRPALKSRKMASVSTFDESALELEAEDLLETLDA
                     DEAAPAEAQTQLRPHRTTTPPVFQQQATSDVPRPGAMSVEVSLTELATLTDRNRIVEV
                     LLGHAALIFDVAVLFAVRDQMAFGWKAFGAAPGVQHVEHLLIPLDAPSVVQAATAAEG
                     GLIHGPVTPSTVNNYLYKVLGCSEPRHATAGVVMIGKRVVNILYGHGRELTPMQIEDL
                     RAVCIAAAEAYARLIAVQKKKK"
     gene            2951..4402
                     /locus_tag="VFV99_11650"
     CDS             2951..4402
                     /locus_tag="VFV99_11650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5060009.1"
                     /translation="MRSAVYGLCMLVAACGSKKERDKPVPAPGAQALPAPDEPAPPSP
                     PDFPEGTRSLQLSRTTPVRLDPGDDGKRIGTVAVDTRVGWTRTASGKGCTKPWVEIVP
                     RGWVCGDSLKPTKQPPFGREVPQLDRGEIVPGIYGKLSGQNLSTYRLELPEDKKPKKP
                     NKERPVTQPSQVEAPKEPRMVEDKPIIGSLTVRQYEQLTVGGKVYWKIQPKGNEYVLK
                     SAVHVHTPSQFMGTRLGDDTGWALPIAFVWPRNGWQQAWTYHKPNAIVNRQVPARMAV
                     PILEVSAGKDGKPVAYRIGNEEWMSPQDLRIFEPTAPPPLLKPNERWIDVDLDKQILV
                     AFEGELPVYATLVSSGIKDTPTQTGLYRIWLKESEADMKNLKAEDPYSVSTVPWTQFF
                     YPEDDLALHTAYWHDGFGKPRSHGCVNLAPRDARWLYYWSDPQMPPGWTMTTGVVEAP
                     GSVVRVRSAADPNPEWKGYAKKVVEARQQRAPI"
     gene            complement(4595..5248)
                     /locus_tag="VFV99_11655"
     CDS             complement(4595..5248)
                     /locus_tag="VFV99_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060010.1"
                     /translation="MSYEPEVALTHPRFHRVEVAVFTKRVVADCMSHRCAMHETHTEK
                     LDACCQYGCDVDLTERDAILARRDAIAPVLAAEVRDLPWFDESSPETDPDTPSGTVVR
                     TAVHRDGCLFLAHDGRGCAIHRAAVEQGWDFRGVKPSICRLFPLTYGDGAIMVSDDYP
                     DYSCAYEPGAPTLYRVAREALRDIFGDDLVHAMDAAEALVLAAEPKRLPVLRPRSEL"
     gene            complement(5264..5455)
                     /locus_tag="VFV99_11660"
     CDS             complement(5264..5455)
                     /locus_tag="VFV99_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060011.1"
                     /translation="MRDDRSLPVIQACSDNFTQHGPAAADADCILDQTTLSAMVLAVD
                     RDAGNDGARIDLAAVLADC"
     gene            5803..6702
                     /locus_tag="VFV99_11665"
     CDS             5803..6702
                     /locus_tag="VFV99_11665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5060012.1"
                     /translation="MDLNDIVVFTKVVETKSFTGAADALGLPKSTVSRKLAQLEERLG
                     VRLVQRTTRKLALTEIGEAYYERCSRIVADIHAAEQLVTDMQSTPRGRLRVTASVDFS
                     TRFLGEIVAEFIAQHPEINVELEATDRVVDLIEEGFDMAVRFGQMPESTLIARKLCGL
                     HLILCASPSYLARRGTPRGIEELDDHDHVLFTPSTRNQTWTLVGPNEQTYEFGRPARL
                     ASNNYGAVVDATRAGSGIASISEFMVTEEIKSGVLVRVLPEWQTRPTEVHAVYPARQN
                     VPPRLTLFLEHLAKSLNPPPWAR"
BASE COUNT         1124 a         2246 c         2327 g         1114 t
ORIGIN      
        1 agtcgctggt ggccgcaagc cgagcgcagc gatcacggtc cattcacgcg ccacggcgtg
       61 cgcgcggtcc acttctacaa ccgcggcaac gacggcgact ggatcgatct cgcgtatcac
      121 agcgcgcgcg atgtgcctgc gcgactgcat cgcgattcgc tcgccgagac cgcgcgcctg
      181 ttgcgtgcgc tcgtcgcgac atcgccacct gcacactctg gcgatggctt ctggttgcca
      241 ctcgcgcgca acacggtcgt gccgcgctgg acgttgatcg tgggcgagag cgtgctgata
      301 ctcgtcgtgc tcgtcgcgct cgtgctgtcg cgcgagggac tcgtcgccgc actcgcacgc
      361 cgctcgcaac gcgcgaccac gccgccggcg tcaggccttc tcgcaggcgc gggctgctac
      421 gtgctggcga tcgtgttctc gaccgtgttc gagcgcagct tcgcgagcgg tcatcccgca
      481 ccgtggttgc acgagccact gcgcgcactc gtcggcacga tgctcgtgct gctcggagcg
      541 ctcggtctcg cgacgcgcgt tgccgcacgc attcggccat ggtgcggtgg tcagcggtat
      601 cgcgcgctcg cagcgatcac gtgcacgttg atcggcgccg cgctcctcgt cgtcggtgca
      661 gccgagctcg catgggtctg gctcgtgccc gccgccgtga tcgcggtgat accggcacgc
      721 gccgcggtca tcgcagtgct cgcatccgcg ctgccgatcg cgtgtgtgat gaacccgtgg
      781 cagctgcggg aggcggcctg gaacggtttc ctcccgctgt cactcccgtt gtctgtattg
      841 ctaggaatac ttgccgtccc aaccatcgcg acgctcgcgt ggtggctgcg ccgccgcacg
      901 ccatccggac cgctggggac actcgtcctg ggcatggggt gtggactcgc cgtgatcatc
      961 ggactcgcgt ttgcggccac gagccgacct gcgtgcagcc ccgcgaaatt cgaaacgttt
     1021 cacctcgcat gcgagcgcgt ctagctattg catgaccgct cctgttacaa cggatcttcg
     1081 actggggagc tcatggctcg aattgtcgtc atcttttcgc ttgttctcgc ggcttgtgat
     1141 gttggtgagc tgccaggtgt aggtggcgcc gatggtggta gtggcagcgg cgatggtggc
     1201 ggcaacgggt gtatcgaggt gtccgcgacg cctgcggtcg gtcaccacaa tccggtcatg
     1261 ccaaccggct gcatgagcgc ggccgggtgc cacagccagg cgaagggcct cggcgcggcg
     1321 gcgccggcct atagctatgg cggcctcctg tacaaagccg acaagatgac gccgtacgcg
     1381 ggcgcgaccg tcatcatcaa gctcgggacc gcggagaaga aggccgccgt tgccgacaac
     1441 ggcgagttct tcctggtgcc cggcatcgcc gggctcgacg agcccacggc gacgatgacg
     1501 gggtcgacgg aggcgagcgc atgcccgagc gcgaacttga agatgggcgg tctgctcacg
     1561 gccgcgggcg gcgactgcaa cagcgccgca tgtcaccagc ccggtggcgt gcaaggcgtg
     1621 atctacgcgc cgtagctatt tcttcttctt ctgcaccgcg atcaggcgtg cgtacgcctc
     1681 ggcagcagcg atgcagaccg cgcgcagatc ttcgatctgc atcggcgtca gctcgcggcc
     1741 gtgcccgtag aggatgttga cgacgcgctt gccgatcatc acgacgccgg ccgttgcgtg
     1801 gcgcggctcc gagcagccga gcactttgta gagatagttg ttcaccgtgc tcggcgtgac
     1861 cggcccgtgg atcaggccac cttcagcggc ggtagcggct tgcacgaccg acggtgcatc
     1921 gagcgggatc agcaaatgct cgacgtgctg cacgccgggc gcggcgccaa atgccttcca
     1981 accgaacgcc atctgatcgc gcaccgcgaa caacacggcg acgtcgaaga tcagcgccgc
     2041 gtgaccgagc agcacctcga cgatgcgatt gcgatcggtg agcgtcgcga gctccgtcag
     2101 cgacacctcg acgctcatcg cgccagggcg cggtacatca ctggtcgctt gctgctggaa
     2161 gaccggcggc gtcgtcgtgc gatgcgggcg aagctgcgtc tgcgcttcgg ccggcgcggc
     2221 ttcgtcggca tcgagcgtct cgagcaagtc ctcggcttcg agctcgagcg ctgactcgtc
     2281 gaacgtcgag accgaggcca tcttgcggct cttgagcgcg gggcgtggac cgggcgcggc
     2341 gaccggtgcg gccggcaccg gcggcaaacc gggcagcggc ggcgcgggca ggcccgcatc
     2401 ggcgacgttg tcgttgccgc gcggtgtgtc gccgaacttc atgaagcgcg cgggccgcgc
     2461 cacgccgtag taccgctcga tgtagtagta gacgcggacc tcgggcgcga cgcgcgggag
     2521 cacgcggtac ccggtgatct gggtcagcgc ttcgagcgtg gcgaagtcgt gcgggtcctc
     2581 gatcgccgcg atcaactggc ggtcctgcac gacgagcggc acgcacttgt gcttgaacgc
     2641 ctgcgtcggc tggagcagcc gcaccgcggc acgctttgcg cgctcgagca tcgcgccggt
     2701 cgcgatcgga ataccgagct cgagcccgag atagatcgtc agcgcctcca gatccacgag
     2761 gccgtgctcg tagagcaccg tcccgagccg gccgccgtcc cgcgcctgaa acgccagcgc
     2821 ctgggaaagc tgcggctcgg tcagacgacc gtcgcgcacg agcatctcgc ctagtcgcat
     2881 ccgattttga cggtagcatg gctgatccgg ccgtctggag tttcgggacg agcgtggtat
     2941 tgcaggatcc gtgaggagcg ccgtctacgg gctctgcatg ctcgttgccg catgcgggtc
     3001 gaagaaggaa cgggacaagc cggtaccggc gcccggcgcc caggcgttgc ccgcccccga
     3061 cgagccggct ccaccctccc caccggattt tcccgagggg acccggtcgc tgcagctgag
     3121 ccgaacgacg ccggttcggc tcgaccccgg cgatgacggc aagcgcatcg gtacggtcgc
     3181 ggtcgacacc cgggtcggct ggacccggac cgcaagcggt aagggctgca cgaagccctg
     3241 ggtcgagatc gtgccgcgcg gttgggtctg cggcgactcg ctgaagccga ccaagcagcc
     3301 gccgttcggt cgcgaggtcc cccagctcga ccgcggcgag atcgtgccgg gcatctacgg
     3361 caagctctcg ggccagaacc tgtcgaccta tcggctcgag ctgcccgaag acaagaagcc
     3421 gaagaaaccg aacaaggaac ggcccgtgac gcagccgtcg caggtcgagg cgcccaaaga
     3481 gccgcgcatg gtcgaggaca agccgatcat cggctcgctc accgtgcgcc agtacgagca
     3541 gctcacggtc ggcggcaagg tctactggaa gatccagccg aagggcaacg agtacgtatt
     3601 gaagtccgcg gtgcacgtgc acacgccgtc gcagttcatg ggcacgcggc tcggtgacga
     3661 caccggctgg gcgctgccga tcgcgttcgt gtggccgcgc aacggctggc agcaggcgtg
     3721 gacctatcac aagccgaacg cgatcgtgaa tcgtcaggtc ccggcgcgca tggcggtgcc
     3781 gatcctcgag gtgtcggcgg gcaaggatgg caagccagtc gcgtatcgga tcggcaacga
     3841 ggaatggatg tcgccccagg acctgcggat cttcgagccg accgcgccgc cgccgctgct
     3901 caagccgaac gagcgctgga tcgacgtcga tctcgacaag cagatcctcg tcgcgttcga
     3961 aggcgagctc ccggtctacg cgacgctcgt gtcgagcggc atcaaggaca cgccgacgca
     4021 aaccggcctg tatcggatct ggctcaagga atccgaggcc gacatgaaga acctcaaagc
     4081 cgaagatccg tactcggtct cgacggtgcc gtggacgcag ttcttctatc ccgaggacga
     4141 tctcgcgctg cacaccgcgt actggcacga cggcttcggc aagccacgca gccacggctg
     4201 cgtgaacctc gcgccgcgcg atgcgcgctg gctctattac tggagcgatc cgcagatgcc
     4261 tccgggctgg acgatgacga cgggtgtcgt ggaagcgccg ggctcggtcg tgcgcgtgcg
     4321 ttcggcggcc gacccgaatc ccgagtggaa gggctacgcg aagaaggtcg tcgaagcgcg
     4381 ccagcagcgc gcgccgatct agagcgagcc aacgcgtgac cgaagaccct cgtcgacgcg
     4441 acgttcacgc cctcgatgac gagggcaacg tgctgtgcaa cccacgggat cgcgaggccg
     4501 ctcaccgggc cgacgttggc gacgtcgcga cctccgccga gcccgcggcc gtgacctgca
     4561 ggaaatgtct cgaccggctc cgccgatcgg cgggttagag ctcggagcgc ggccgcagca
     4621 cgggcaggcg cttcggttcg gcggcgagca cgagcgcttc agctgcatcc atcgcgtgga
     4681 cgaggtcgtc gccgaagatg tcgcgcaggg cttcgcgcgc gacgcgatac agcgtcggtg
     4741 cgccgggctc gtaggcgcac gagtaatcgg gatagtcgtc ggagaccatg atcgcgccgt
     4801 cgccgtaggt gagcgggaac aggcggcaga tcgacggctt cacgccgcgg aagtcccagc
     4861 cttgctcgac ggcggcgcga tggatcgcgc agccacggcc atcgtgcgcg aggaacaagc
     4921 agccatcgcg gtggacggcg gtgcggacga ccgtgcccga cggcgtgtcg ggatcggtct
     4981 cggggctgga ctcgtcgaac catggcaggt cgcgcacctc ggcggcgagc accggcgcga
     5041 tcgcatcgcg gcgggcgaga atcgcgtcgc gttcggtgag gtcgacgtcg cagccgtact
     5101 ggcaacacgc gtcgagcttc tcggtgtgcg tctcgtgcat cgcgcagcga tggctcatgc
     5161 aatcggcgac gacgcgcttc gtgaagaccg ctacttcgac tcgatgaaac cgcgggtgag
     5221 tgagcgcaac ttcaggctcg tacgacatca ggggtggcgg aatctagcag tcggcaagga
     5281 cggcggcgag atcgattcgc gcgccatcgt taccggcgtc gcgatcgact gcgagcacca
     5341 tggccgacag cgtggtctgg tcgagaatgc aatccgcgtc ggctgcggca gggccgtgct
     5401 gagtgaaatt gtctgaacag gcctggatta cggggagtga gcggtcgtcg cgcatccgga
     5461 tcggtgcacc atgcgaacgg cacacgatcg gacggacctg atagatcagg cagcgaccgt
     5521 tggcgtccag ggcgctgcag tggtcttgtg gtgaccctgt cacattcgcg gtgagggctt
     5581 gcttgcgctc tgggggccaa ccagcgacct cggcgcggat cgcgtcggcc tcgaccttcg
     5641 tgaccgaaag ccggacgtgg cagcaatgat gacaaccggt cccacatttc atgtcttcgc
     5701 cgtgtctcgc ctggacgcgc gcgaagaacg cgtctacttt cgctgcgagg tcggggtagc
     5761 gagacattcc ggccgagtgt agaacacctc gaagagtcgg ccatggacct caacgacatc
     5821 gtcgtattta ccaaggtcgt cgagaccaag agctttaccg gcgccgccga cgcgctgggg
     5881 ctcccgaaat cgacggtcag ccgcaagctc gcccagctcg aggagcgact cggcgtgcgc
     5941 ctcgtgcagc gcacgacccg caagctcgcc ctgaccgaga tcggcgaggc gtattacgag
     6001 cggtgctcgc gcatcgtcgc cgacatccac gcggcggagc agcttgtcac cgatatgcaa
     6061 tcgacgccgc gcggccgcct gcgcgtcacc gcgtcggtcg atttctcgac gcggtttctt
     6121 ggcgagatcg tcgccgagtt catcgcgcag catcccgaga tcaacgtcga gctcgaagcg
     6181 accgatcggg tcgtcgatct gatcgaagaa ggcttcgaca tggcggtgcg cttcggccag
     6241 atgccggagt cgacgctgat cgcgcgcaag ctgtgtggcc tgcacctgat cttgtgcgcg
     6301 tcgccgtcgt acctcgcacg gcgcggcaca ccacggggca tcgaagagct cgacgatcac
     6361 gaccacgtgc tgttcacgcc gtcgacgcgc aaccagacct ggacgctcgt cggccccaac
     6421 gagcagacgt acgaatttgg ccgcccggcc cggcttgcga gcaacaacta tggcgccgtc
     6481 gtcgacgcga cgcgcgcggg cagcggcatc gcatcgatct cggagttcat ggtcaccgag
     6541 gagatcaaga gcggtgtgct cgtgcgcgtg ctgcccgagt ggcagacccg gccgaccgaa
     6601 gtgcatgcgg tgtatcccgc acgccagaac gtgccgccac gcctcaccct gtttctcgaa
     6661 cacctcgcga agtcgcttaa cccgcccccg tgggcgcgtt aagaacgcgg catgcacatc
     6721 acctgggctg atttcgaagc attcgcccaa gcgcacctgg tcccgctcgg gctgcgattt
     6781 ctcctcgcgc tgatcatctt cgtcgccggc c
//
LOCUS       DASUFX010000297         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_740933,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000297 DASUFX010000000
VERSION     DASUFX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740933"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VFV99_11670"
     CDS             complement(<1..1416)
                     /locus_tag="VFV99_11670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060013.1"
                     /translation="MQPAPLNQKFGRYELLTMLARGGMAEVFLARMSGVGGFAKLLVI
                     KRILPHLNDDPEFHEMFLNEGRVTARLNHSNICQVYELGEVDGVVFLAMEYLEGLPWS
                     DLAPLLPRGRGVELRVAATVLGQICEGLRFAHEYADVDGKPMPIVHRDVSPQNLFITN
                     EGVCKLLDFGVSKVLTEGTRTRTGMLKGKLPYMAPEQIRGEPIDARADVFSMGVVLWE
                     ALTGARLFNRDSDYQIWKAITEEEVPRVTSRVPGLPVTIDGVVMRALERDVNRRYPSI
                     RAFAGELREVAERAGGTFDTATLSQILKSLGAGKLADKNEKVSRAIGRPARASEPPPV
                     PAAITQPLPTIEPSVTQHSIALRDASVKLGRRKPRLRIAIAVGVLAIVVGITAALWTR
                     KDPAVADAQRDAAAVVAVATGDAEQQGSATDDVEIDEPPADDPDHNEPDKPVADEPDK
                     PARPRPVRRPKKVVEHKPPVDA"
     gene            complement(1432..2541)
                     /locus_tag="VFV99_11675"
     CDS             complement(1432..2541)
                     /locus_tag="VFV99_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5060014.1"
                     /translation="MLIVALEVRAVVVGCFVATDREQQDPHAAHVARLMEYSGRVEVR
                     RVRCVINVPNGPSRRVGPNGLLIGRKQDCDLVADDPSVSRRHALVRLTSEGAELVPLG
                     RGVVELNGKKLDKPTALADGDQLGVPGLVLEVQIDAQRPDAKASASWRLERERGGSFG
                     LVHSPFVIGGDDGDDLIVKRWPDHALRFHIAQGELYIEPTVGKATRNGSELEVGAMES
                     LLVGDTIAFRKEAFVVRQAPRDATTAVASLNDVPTHVLIEMLPRGGRVVLSTAEGERA
                     VFLADKRLDLVIALLRPPAGFAPGDFIPDDVVRPIVWPRNPGVTRPEINVLISRCRRD
                     LIEAGLAGARLLERSPGGGGTRFALAPGATVVVES"
     gene            2452..>2720
                     /locus_tag="VFV99_11680"
     CDS             2452..>2720
                     /locus_tag="VFV99_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060015.1"
                     /translation="MRCVWILLLAIGCYEAPDYNGTHFKCDDQHACPDGQQCVNGICG
                     GSGSGSNMIDAPQSMSGVHCGAATCTSSQKCCADLVSTSVSCIPLT"
BASE COUNT          449 a          939 c          869 g          463 t
ORIGIN      
        1 ggcatcgacc ggcggtttgt gctcgacgac tttcttcggc cggcgcaccg gccgcgggcg
       61 cgcgggcttg tcaggctcgt ctgccaccgg cttgtcgggc tcgttgtgat cggggtcatc
      121 ggctggcggc tcgtcgatct cgacgtcgtc cgtcgcggag ccctgctgtt ctgcatcgcc
      181 ggtcgcgacg gcgaccacgg cggcggcatc ccgctgcgcg tcggcaacgg ctgggtcttt
      241 ccgcgtccac agtgcggcag taatgccaac cacgatcgcg agcacgccga cggcgatcgc
      301 gatgcgcagc cgtggtttgc ggcgcccgag cttcaccgat gcgtcccgca gtgcgatcga
      361 gtgctgggtc acgctcggct cgatggtcgg cagcggctgc gtgatcgcag ctggcaccgg
      421 cggcggttcg ctcgcgcgcg cgggccggcc gatcgcgcgg ctcactttct cgttcttgtc
      481 ggcgagcttg ccggcgccga gcgacttcag gatctggctc agcgtcgcag tgtcaaacgt
      541 gccgcctgcg cgctcggcga cctcgcgcag ctcgccggca aacgcacgga tcgacggata
      601 gcgccggttc acgtcgcgct cgagtgcgcg catgaccacg ccgtcgatcg tgaccggcaa
      661 gccaggcacc cgcgatgtca cgcgcggcac ttcctcctcg gtgatcgctt tccagatctg
      721 atagtcgcta tcgcgattga acaggcgcgc cccggtgagt gcttcccaca gcacgacgcc
      781 catcgagaac acgtcggcgc gcgcgtcgat cggctcacca cggatctgct ccggcgccat
      841 gtacggcagc ttgcccttca gcatgcccgt ccgcgtgcgc gtgccttcgg tgagcacctt
      901 cgacacgccg aagtcgagca gcttgcagac gccctcgttc gtgatgaaca agttctgcgg
      961 cgatacatcg cgatgcacga tcggcatcgg tttgccgtcg acgtcggcgt actcgtgcgc
     1021 gaaccgcagc ccttcgcaaa tttgcccgag caccgtcgcc gccacgcgca attcgacgcc
     1081 gcgcccacgc ggcaacagcg gcgccagatc cgaccacggc agaccttcga ggtactccat
     1141 cgcgaggaac acgacgccgt cgacctcgcc gagctcgtag acctggcaaa tattcgagtg
     1201 attgaggcgc gcggtgacgc gaccctcgtt gaggaacatc tcgtgaaact cggggtcgtc
     1261 gttgaggtgc ggcaggatcc gcttgatcac gagcagcttc gcgaagccgc cgacgcccga
     1321 catccgcgcg agaaacacct cggccatacc gccacgggcc agcatcgtga gcagctcgta
     1381 gcgcccgaac ttctgattga gcggcgcggg ttgcagagcc tcaacagtac atcagctctc
     1441 gaccaccacg gttgcgcccg gtgcgagcgc gaaccgcgtg ccaccaccac cgggagaacg
     1501 ttctaataac cgcgcgcctg cgagccctgc ctcgatgaga tcgcggcggc atctactgat
     1561 gagtacgttg atctccggcc gcgtcacgcc aggattgcgc ggccacacga tcggccggac
     1621 gacgtcatcc gggatgaaat cgccgggcgc aaacccggca ggcggcctca gcagcgcgat
     1681 cacgaggtcg aggcgcttgt cggcaagaaa caccgcgcgc tcgccttcgg ccgtcgacag
     1741 cacgacgcga cctccgcggg gcagcatctc gatcaagacg tgcgtcggca cgtcgttgag
     1801 actcgcgacc gcggtggtcg catcgcgcgg cgcctggcgc accacgaacg cctccttgcg
     1861 aaatgcgatc gtatcgccga cgagcagcga ctccatcgcg ccgacttcga gctcgctgcc
     1921 gttgcgcgtc gctttgccga ccgtcggctc gatgtagagc tcgccttgcg cgatgtgaaa
     1981 gcgcagcgcg tgatcgggcc aacgcttgac gatcaggtcg tcaccgtcgt cgccgccgat
     2041 cacgaacggg ctgtgcacga ggccgaagct gccgccgcgc tcgcgctcga gccgccagct
     2101 cgcggacgct ttcgcatcgg gacgttgcgc gtcgatctgc acctcgagca cgaggcccgg
     2161 tacgccgagc tggtcgccat cggcaagcgc ggtcggttta tcgagcttct tgccgttgag
     2221 ctcgaccacg ccgcgcccga gaggaaccag ctccgcgccc tccgaggtca atctaacaag
     2281 cgcatgacgg cggctgacac ttgggtcgtc cgcgacaagg tcgcaatctt gtttgcggcc
     2341 gatgagcaag ccattcgggc caacacgacg cgagggtccg ttcggtacgt tgatcacgca
     2401 tcgcacacga cggacttcca cacgcccact gtattccatt agccttgcta cgtgcgctgc
     2461 gtgtggatct tgctgctcgc gatcggttgc tacgaagcac ccgactacaa cggcacgcac
     2521 ttcaagtgcg acgatcagca tgcctgtccg gacggccagc agtgcgtcaa cggaatctgc
     2581 ggtggcagtg gcagcggcag caacatgatc gacgcgccgc aatcgatgag cggcgtgcac
     2641 tgtggtgcgg cgacgtgcac gagcagtcag aagtgctgcg ccgatctcgt gagcacctcc
     2701 gtctcgtgca tcccgctcac
//
LOCUS       DASUFX010000298         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1434181, whole genome shotgun sequence.
ACCESSION   DASUFX010000298 DASUFX010000000
VERSION     DASUFX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434181"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2115)
                     /locus_tag="VFV99_11685"
     CDS             complement(40..2115)
                     /locus_tag="VFV99_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060016.1"
                     /translation="MSACPPVDKLLAFASGELTDDKSAEVEGHIDSCAECRAALSNYA
                     KGGPPPVLGRYRIDTVLGSGGMGIVYRAYDPQLARPVAIKVVRRAGEDEAGRARLIRE
                     AQALARLSHPNVCHVYDVGSEGDEVWVAMELIDGVSLRAWAEEHRDRDKLVGVLLGAA
                     EGIAAAHAAGLVHRDIKPENVLVTRDGRAIVTDFGLARHEDAINPHASTLSNDPHLTA
                     TGAIAGTPAYLAPEQLLGDPIDSRVDQFAWAVMAYEMLTGSKPFPIIFAVRVEAIRQG
                     LTPPASLPSYIANALVRAMAASPSDRFASMRELIAAMKAQQATVPRRGRAPAVAGLVV
                     LGAGALVLGAWQLTKAGDEPKPVQVAAAKNISPLPNETATPPIPAAADAATAAPMPSP
                     SPPSPTTNVAAPAATTNLQVPSPSPIGSAKKPKPTAKTDQAMQASVTPAPAPTGAGAG
                     SNAQLTPRQQQILDSLDPPYCRSCAIASIDAFCEIPYDFTNPETSRKHEVVDWGKVTK
                     VAVENGKLGDRPISLNIITVQGQRDTYRFKDELLVGRLETQVGALVAICPEEEDDFYQ
                     LSGGPTFKTRAVATLSAAPKMTDAAKYKALHIPDIRIAAAAVNKSLDRLDPNRRYLIN
                     AKVEADDGSLWKMDRYWLDASGAKNASRLAAKKRYWMIVEKPDFFESVPGDKRLVVHA
                     VAILDDIFP"
     gene            complement(2112..>2836)
                     /locus_tag="VFV99_11690"
     CDS             complement(2112..>2836)
                     /locus_tag="VFV99_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060017.1"
                     /translation="NRLAQRVQDDPETKLDALHDADLYLAMALADNNEAALRVFEAEM
                     VPQIDVALRRLRLAGGTADEVKQALRFELLVGVDGHRRIADYAGRGELAAWLRVSATR
                     KAFKVIRKIDREETLDEILLDHWPDATPGPEQKQLRSQYTAELKKAIREAFAALEVRQ
                     RNLLRQHILDELTIDDLARLYRVHRATCARWLADARADLGKLTRKKLMAALGMPTNDV
                     DSLLRFLDSDIELSISRILMSR"
BASE COUNT          419 a          917 c          995 g          505 t
ORIGIN      
        1 gtcgcacata aaatgcgacg gccgagcaaa tcgcgagaac tacgggaaga tgtcgtcgag
       61 gatcgcgacc gcgtggacga cgagccgctt gtcaccgggc accgactcga agaagtcggg
      121 cttctcgacg atcatccagt agcgcttctt ggcagcgagc cggctcgcat tctttgcacc
      181 cgaggcgtcg agccagtagc ggtccatttt ccaaagtgag ccgtcatccg cctcgacctt
      241 tgcgttgatc aggtaacgtc ggttcgggtc cagccggtcg agactcttgt tcacggcggc
      301 ggcggcgatc cggatgtccg ggatgtgtag cgccttgtac ttcgccgcgt cggtcatctt
      361 cggtgccgcc gacagcgtcg cgacggcgcg cgtcttgaac gttggcccgc ccgagagctg
      421 atagaagtcg tcctcttctt cggggcaaat tgcgacaagc gcgccgacct gcgtctcgag
      481 ccggccgacc agcaactcgt ccttgaagcg atacgtgtcg cgctggccct ggacggtgat
      541 gatgttgagc gagatcgggc ggtcgccgag cttgccgttc tcgacggcga ccttcgtgac
      601 cttgccccag tcgacgacct cgtgtttgcg gctggtctcg gggttcgtga agtcgtacgg
      661 gatctcgcag aacgcatcga tcgacgcgat cgcgcagctg cggcagtagg gcggatcgag
      721 cgagtcgaga atctgttgct gccgcggcgt gagttgcgcg ttgctgccgg ctccggctcc
      781 ggtgggcgcg ggtgctggcg tcaccgacgc ctgcatcgct tgatcggtct tcgctgtcgg
      841 ctttggcttc ttcgcgctgc cgatcggcga gggcgacggc acctgcaggt tggtggttgc
      901 ggcgggtgca gccacgttcg tcgtcggcga tggcggcgac ggcgatggca tcggcgcggc
      961 ggtcgcagcg tcggcagctg cggggatcgg cggcgtcgcg gtttcgttcg gcagcggcga
     1021 gatgttcttc gcggcggcga cttgcaccgg cttcggctcg tcgccggcct tcgtcagctg
     1081 ccacgcaccg agcacgagcg cgccggcacc gagcacgacg agaccggcga cggcgggtgc
     1141 gcggccacgg cgcggcacgg tcgcttgctg cgctttcatc gccgcgatca gctcgcgcat
     1201 cgaggcgaag cggtcggacg gcgacgctgc catcgcacgc acgagcgcgt tcgcgatgta
     1261 gctgggcagg ctcgccggtg gcgtcaggcc ctggcggatc gcctcgacgc gaacggcgaa
     1321 tatgatcggg aacggcttgc tgccggtgag catctcgtac gccatcaccg cccacgcgaa
     1381 ctgatcgacg cgcgagtcga tcggatcgcc gagcaactgt tcgggcgcga ggtacgcggg
     1441 tgtgccggcg atcgcgccag tcgcggtcag atgcggatcg ttgctcagcg tcgacgcgtg
     1501 cggattgatc gcatcctcgt gacgcgcgag cccgaagtcg gtgacgatcg cgcggccgtc
     1561 gcgcgtgacg agcacgttct ccggtttgat gtcgcggtgg acgagaccgg cggcgtgcgc
     1621 ggccgcgatg ccctcggcgg cgccgagcaa cacgccgacg agcttgtcgc gatcgcgatg
     1681 ctcctcggcc cacgcgcgca gcgaaacgcc gtcgatcagc tccatcgcga cccagacctc
     1741 gtcgccttcg ctgccgacgt cgtagacgtg gcacacgttc ggatgcgaga gccgcgcgag
     1801 tgcttgcgct tcgcggatca gccgcgcgcg accagcttca tcctcgccgg cgcggcgcac
     1861 gaccttgatc gcgaccggcc gtgcgagctg cggatcgtac gcgcgataca cgatgcccat
     1921 accgccggag ccgagcacgg tgtcgatgcg atagcggccg agcacgggcg gcgggccgcc
     1981 cttcgcgtag ttcgacagcg ctgcgcgaca ttccgcgcag ctgtcgatgt ggccctcgac
     2041 ttccgccgac ttgtcgtcgg tgagctcgcc gctggcgaac gcgagcagtt tatcgacggg
     2101 agggcaggcg ctcatcggct catcaagatg cgcgagatcg agagctcgat gtcgctatcc
     2161 agaaagcgca gcaagctgtc gacgtcgttc gtcggcatac cgagcgctgc catcagcttc
     2221 ttacgcgtga gcttgccgag gtcagcgcgc gcatcggcga gccaacgtgc acacgtagcg
     2281 cgatgcacgc gatagagccg ggcgaggtcg tcgatcgtga gctcgtcgag gatatgctgg
     2341 cggagcaagt tgcgctggcg cacctcgagt gcggcgaatg cctcgcggat cgccttcttc
     2401 agctcggcgg tgtactgcga gcgcagctgc ttctgttcgg ggccgggcgt cgcgtcgggc
     2461 cagtgatcga gcaggatctc gtcgagcgtc tcctcgcgat cgatcttgcg gatgaccttg
     2521 aacgccttgc gcgtcgcgct gacgcgcagc caggcagcga gctcgccgcg acctgcgtag
     2581 tccgcgatcc ggcggtggcc atcaaccccg accaacagct cgaagcgcag cgcttgcttc
     2641 acttcatcgg cggtgccgcc ggcaaggcgg agccggcgca gtgccacgtc gatctgcggc
     2701 accatctcgg cctcgaatac gcgcagcgcg gcctcgttgt tgtcggccag tgccatcgcg
     2761 aggtagaggt cggcatcgtg cagcgcatcg agcttcgtct ccggatcgtc ctgcacgcgc
     2821 tgggcgagcc ggttcg
//
LOCUS       DASUFX010000299         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1434246, whole genome shotgun sequence.
ACCESSION   DASUFX010000299 DASUFX010000000
VERSION     DASUFX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434246"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFV99_11695"
     CDS             complement(<1..296)
                     /locus_tag="VFV99_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060018.1"
                     /translation="MRSLLVIVLSALLVPHLVACGDDGGGNNFSKTQCNDGKDNDGDG
                     DVDFPDDLSCASESDDDESAAAAPQCKDGRDNDGDGKHDFPNDPGCFAPNQDDET"
     gene            complement(398..1585)
                     /locus_tag="VFV99_11700"
     CDS             complement(398..1585)
                     /locus_tag="VFV99_11700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX5060019.1"
                     /translation="MKVAASRPRATLRLTKPLRDAIRAGHPWVYDRAVAPAKSLAVGD
                     VVTLVDDKGPIATAIVDPTSPIRARVLDVPDAACDGAWAAARTEKAAARRARDPLLVG
                     CTGRRLVHGEADGCPGLVVDAYTDTAVIVFDGPAATAFWRTRIDDVLAGLERGGATIA
                     HVWLRGERGQRSAGEALRGAPPAEIVISEDEARFGVDVRSGQKTGFFLDQRENRRIVR
                     RHAGGASVLNLFSYTGGFSLHAALGGATRVTSVDIAGPAIAAIEKNLAHSGLDASAHE
                     RVAADAFEFLSRAKAQGRRWDLVIVDPPSFAPSERAKGAALRAYEKLAAAALALVQDG
                     GRFALASCSSHVTEADLLGTMAGLLAPDVRLRISSGAASDHPVLAAFSEGRYLKFLFF
                     DVG"
     gene            complement(1582..2448)
                     /locus_tag="VFV99_11705"
     CDS             complement(1582..2448)
                     /locus_tag="VFV99_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060020.1"
                     /translation="MRMPLLCAALALVPAVAGAKPKQDAKAAGSKAPACGAKVLPLAV
                     GNQWTYNSIAAPTPPNDQIKRLSPPQPKAIQITVKSVEAKGGDTVVTLEEKMTVDRTR
                     DPKKPEIDEYTYESTVTCNNKKFEISPNSYFFAGEPGGVLGLEITKTDHLKGTSLTLT
                     KGGIGEAEWREDLAMTWKRVPVEGSGAKLGSGKLELERRFTPQTPEQITTKTGEMFKA
                     EKLGLITTGRVTLDNPGNPNHKPMELPANWVSQIWLADNVGVVQTLNSYGHMYQLVET
                     NLTSSNPPPTPQ"
     gene            complement(2539..3159)
                     /locus_tag="VFV99_11710"
     CDS             complement(2539..3159)
                     /locus_tag="VFV99_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060021.1"
                     /translation="MKYALLSLTLASLVFGGCHEKEEVTAPPPPLRPEPVPKPDTTVA
                     KDCDATDPASELKPIAFDERSIPEGQRLADQAYGNLHAADSAEVDRMTREKYQGDAVN
                     DFITALAADPYNIKATYGLAAAYAKIGRKQCSINLLTRLLQMRPHPSKKAEVEHYIDQ
                     LLGRKQKLDPDFSDMRRDNRFRELIAKMCADTNDPNCVYGAQKDGR"
     gene            complement(3162..3710)
                     /locus_tag="VFV99_11715"
     CDS             complement(3162..3710)
                     /locus_tag="VFV99_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060022.1"
                     /translation="MRLLVVLALALAVTSSCTPLYGARPEKLKNPEKKKRPAEAEAAP
                     VEVKYVDDCNANFSDDPKKAHPQTQMSKQLFDQAESSIDQADKAKEDSIKVGMLKEAI
                     DKYRNSLIKDPYNVPATLKLAVAYDRVLRKGCAIAMLKRLAALSNNPKWQSEANRNID
                     SIDANAQWFKGYRKDAMQAVGR"
     gene            complement(3796..>4213)
                     /locus_tag="VFV99_11720"
     CDS             complement(3796..>4213)
                     /locus_tag="VFV99_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060023.1"
                     /translation="TVTLWGSGMSELYDGQMTWREKWHMDEHAVQRLGRSVIRAGVSL
                     PMVIIYGLAPKQPAAMVLSLGALVLAGFGLRALVRMRTWGVLAIGAAGGLMLSLAGVQ
                     LLGGHEALALQPALAGSLLVFAAWPWAQPLVRAIRG"
BASE COUNT          656 a         1383 c         1383 g          791 t
ORIGIN      
        1 gtctcgtcat cctggttcgg agcgaagcaa cccgggtcgt tcgggaagtc gtgcttgccg
       61 tcgccgtcgt tgtcacggcc gtccttgcac tgcggtgcag cagcggcgct ctcgtcgtcg
      121 tccgactcgc tcgcgcagct caggtcatcg gggaagtcga cgtcgccgtc gccgtcgttg
      181 tccttgccgt cattgcactg cgtcttcgag aaattgttgc cgccgccatc gtcaccgcac
      241 gcgacgaggt gcggaaccaa cagcgcggac agaacgatta cgagcagcga gcgcattgat
      301 gcctctctcg gaatggaggc aggtctgtaa cgaactgttg agatcgggtc aagtagttaa
      361 attcgaatgc aaatgcgcac tcgcagtggc tactcgacta gccaacgtca aagaacagga
      421 actttaggta acggccctcg ctaaaggcgg ccagaaccgg gtgatcgctt gctgcgcccg
      481 acgagatccg caggcgaacg tcgggcgcaa gtagtccggc catcgtgcca agcaggtcgg
      541 cttccgtcac atgactcgag cacgaggcga gtgcgaaacg tccgccatct tggacgagcg
      601 cgagcgccgc ggcggcgagt ttctcgtacg cacgaagtgc ggctcccttg gcgcgttcgc
      661 tcggcgcgaa gctcggcggg tcgacgatca cgagatccca acgccggccc tgcgccttgg
      721 cgcgcgagag aaactcgaac gcatcggctg cgactcgctc gtgcgcgctc gcatcgagtc
      781 cgctgtgcgc gaggttcttc tcgattgcgg cgatcgcggg acccgcgatg tcgacgctcg
      841 tcacgcgggt cgcgccgccg agtgccgcgt gcagtgagaa gccgccggtg tacgaaaaca
      901 gattgagcac gctcgctccg ccagcgtgcc gacggacaat gcggcgattc tcgcgctgat
      961 cgagaaagaa gccggtcttc tgaccgctgc gcacgtcgac gccgaagcgc gcctcgtctt
     1021 cgctgatcac gatctccgcg ggtggcgcac cgcgcagcgc ttcgccggcg ctgcgttgcc
     1081 cgcgttcgcc acgtagccac acgtgcgcga tcgtcgcgcc accgcgctcg agaccggcga
     1141 gcacgtcatc gatgcgcgtc cgccagaacg cggtcgcggc cggtccgtcg aacacgatca
     1201 ccgcggtgtc ggtgtacgca tcgaccacga gcccggggca gccatcggct tcgccgtgca
     1261 cgagccgccg cccggtgcag ccgacgagca acggatcacg cgcgcgtcgg gctgcggctt
     1321 tttctgtgcg tgctgctgcc cacgcgccat cacatgcggc atccgggaca tcgagcacac
     1381 gcgcgcggat cggcgaggtt ggatcgacga tcgccgtcgc gatcggtcct ttgtcgtcga
     1441 cgagcgtgac gacgtcgcca acggcgagcg acttcgccgg cgcgaccgca cggtcgtaga
     1501 cccacggatg gcccgcgcga atcgcatcgc gcagtggctt ggtcagccgc agcgtcgcgc
     1561 gcgggcgcga tgcggcgacc ttcactgcgg ggtcgggggc gggttcgagc tcgtcaggtt
     1621 cgtctcgacg agctgataca tgtggccgta gctgttcagc gtctgcacga cgccgacgtt
     1681 atcggccagc cagatctggc tgacccagtt cgccggcagc tccatcggtt tgtgattcgg
     1741 gttgcccggg ttgtcgagcg tcacgcggcc cgtcgtgatg agaccgagct tctcggcctt
     1801 gaacatctcg ccggtcttcg tcgtgatctg ctccggggtc tggggcgtga agcggcgctc
     1861 gagctcgagc ttaccgctgc cgagcttggc acccgagccc tcgaccggga cgcgcttcca
     1921 cgtcatcgcg aggtcttcgc gccactcggc ttcgccaatg ccgcccttcg tgagcgtgag
     1981 gctcgtgccc ttgagatgat ccgtcttggt gatctcgaga ccgagcacgc cgcctggctc
     2041 acccgcaaag aagtagctgt tgggcgagat ctcgaacttc ttgttgttgc acgtgaccgt
     2101 cgactcgtac gtgtactcgt cgatctccgg cttcttcggg tcgcgcgtgc ggtcgaccgt
     2161 catcttctct tcgagggtga cgaccgtgtc gccgcccttg gcctcgaccg atttcaccgt
     2221 gatctgaatc gcctttggct gcggcggcga caggcgcttg atctggtcgt tcggtggcgt
     2281 cggggccgcg atcgagttgt aggtccactg attgccgacc gcgagcggca gcaccttcgc
     2341 gccgcacgcc ggggccttgc taccggccgc tttcgcgtcc tgtttgggtt tggcacccgc
     2401 gacagcaggc accagggcga gggcggcgca aaggaggggc attcgcatag gccccggagt
     2461 catacccacg gaaagcggcc gcgtaaagcg atccgggctg acaggctgcc gcctgccagc
     2521 aacacttgcg actcaggctt atcggccgtc tttttgggcg ccgtacacgc agttagggtc
     2581 gttcgtgtcg gcgcacatct tggcgatcag ctcgcggaag cggttgtcgc gccgcatatc
     2641 cgagaaatcg ggatcgagct tctgcttccg gccgagtagc tggtcgatgt agtgctcgac
     2701 ctcggccttc ttgctcggat gcggccgcat ctggagcagg cgcgtcaata gattgatcga
     2761 gcactgcttg cggccgatct tcgcgtacgc cgcagcgagg ccgtacgtcg ctttgatgtt
     2821 gtatgggtcg gcggcgagcg cggtgatgaa gtcgttgacc gcatcgccct ggtacttctc
     2881 gcgcgtcatg cgatcgacct cggcgctatc cgccgcatgg aggtttccgt acgcctggtc
     2941 ggcgagtcgt tggccctcgg ggatcgaccg ctcgtcgaat gcgatcggct tgagctcgga
     3001 tgccggatca gtcgcgtcgc aatccttggc gaccgtggtg tccggtttcg gcaccggctc
     3061 gggacggagc ggcggcggtg gtgcggtcac ttcttctttc tcgtgacagc cgccgaacac
     3121 cagcgacgcg agcgtgagcg agagcagggc gtacttcatc gctagcgtcc aactgcctgc
     3181 atcgcgtcct tgcgatagcc cttgaaccac tgcgcgttgg cgtcgatcga atcgatgttg
     3241 cgattcgctt cgctctgcca cttcggattg ttcgagagcg cagcgagccg cttgagcatc
     3301 gcgatcgcac agcccttgcg cagcacccgg tcgtacgcga ccgcgagctt gagcgtcgcc
     3361 ggcacgttgt acgggtcctt gatcagcgag ttgcgatact tgtcgatcgc ctctttgagc
     3421 atgccgactt tgatcgagtc ttctttggcc ttgtcggcct gatcgatcga ggactcggcc
     3481 tgatcgaaca gctgcttcga catctgcgtc tgcgggtgcg ccttcttcgg atcgtcgctg
     3541 aagttcgcgt tgcagtcgtc gacgtacttg acctcgaccg gcgccgcttc cgcctcggcc
     3601 ggccgcttct tcttctccgg attcttcagc ttctccgggc gagcgccgta gagcggcgta
     3661 cacgacgagg tgacagccag ggcgagcgcg aggaccacca gcaagcgcat acctcgacgg
     3721 tagcgcggcg gcctcggcga atcaaagcgt tacgtgggaa tccgcggacg cgaaccgccg
     3781 gagctggatg accgattatc cgcggatcgc gcggaccagc ggttgcgccc acggccaggc
     3841 cgcgaacacg agcagcgagc cggcgagggc aggctgcagc gccagtgctt cgtggccgcc
     3901 gagcagctgc acgcccgcga gcgacagcat cagaccaccg gccgcaccga tcgcgagcac
     3961 gccccacgtg cgcatccgaa cgagcgcgcg gagaccgaag cccgcaagca cgagcgcgcc
     4021 gagcgatagc accatcgcag cgggctgctt cggtgcgagg ccgtagatga tcaccatcgg
     4081 cagcgagacg ccggcgcgga tcaccgaccg accgagccgc tgcaccgcgt gctcgtccat
     4141 gtgccacttc tcgcgccacg tcatctggcc gtcgtagagc tcgctcatgc cgctgcccca
     4201 cagcgtcacc gtc
//
LOCUS       DASUFX010000300        17216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_741064,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000300 DASUFX010000000
VERSION     DASUFX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17216
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741064"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1131)
                     /locus_tag="VFV99_11725"
     CDS             complement(28..1131)
                     /locus_tag="VFV99_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060024.1"
                     /translation="MVPLLARHTIVLAQQQVIELQQSHACPDAQVRESVRAMRIALLL
                     LFVACTEPPPHVATGELGPWQVGPALPTPRANHCSAVIDDWVLVVGGNYMDSGAFVKT
                     AEIHAARIEEGVLGNWRLAGMLPSPATECSATSDGRTLYVLDGIYDDPAHGRQVWSAT
                     LDETGHLGALASLGMLPATVVSSEATVHADTLVLMDTRLPAEGNATVALQSPITPSLA
                     WQTEELGIDFLAQAEYAFSADNVYTLGGYHDPAQGAVANVFVARLMGGVARATTPLPV
                     PVGFGEAVAVDDWVFVVGGRAAVFGAPGTAQVFAAHATADGSLGAWRESALPMARTNH
                     ELVVAGDYLVVTGGAVIGPGDATVFTAQVRWPP"
     gene            1204..1665
                     /locus_tag="VFV99_11730"
     CDS             1204..1665
                     /locus_tag="VFV99_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060025.1"
                     /translation="MRAAVLSVALTACAYRPGSFSHLSESFPGQRTTVGCIDLSVERR
                     PDLIGGGTVLAYAFGNRCDHPAIVDFTQVKVHARTYDGRDLILAAFDPEGQIQPLELD
                     GRATGEEAIAYKIDASLAEVCVDAASLTHESPARWLCFASNLPPQIAEAGK"
     gene            1662..2546
                     /locus_tag="VFV99_11735"
     CDS             1662..2546
                     /locus_tag="VFV99_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060026.1"
                     /translation="MKYLLFAALLLADPRPVHAGSCHGGGNAGTSSSGSHDDGGRSSS
                     SSNDTSWSYTPSSAGDYVVHSSECIDNTDVHGFRHCSKFGAWGKNMRIPKLFIEVGGN
                     VRNFGSALVDRTGTISHGYESFAYRVVMPGADAPRDVAMTSTLRLGFGIPRGFYSGLE
                     FELGALVSPAAATAEMTTAGAFGTPNVEQQHGMFLGFTGVGGYRAVGSRGAIAIEGAG
                     GLRSVRYNFDSSYHYCETTTTVTANMGVVEARARAELWLSPWLTAGVTVGSNVLERSD
                     WMAGVYFGAHSRAYAGAR"
     gene            complement(2543..3385)
                     /locus_tag="VFV99_11740"
     CDS             complement(2543..3385)
                     /locus_tag="VFV99_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5060027.1"
                     /translation="MKIKFWGVRGSIPAPGPETNRYGGNTSCVSVTTANGGLIIIDMG
                     TGLMHLGNALLASTFGKGQGRATILLSHTHWDHIQGLGFFPPVFIPGNQFRVWGPGNS
                     SEMLEDILEGQMDPNFSPLQTLKNLAATFDVSAMVCGKTIEADGVTISAREHQHGQTT
                     ALAFRLEEAGRVFVYASDVGYPESGPTPEAIEHYSGADVLLHDSTYRPEDQATRRNRG
                     FSSYVDACDAAIAAKAKRLVTFHYDQDYTDDDVDQIVAACKAKLAPHGIAVVPAREGD
                     ELEI"
     gene            complement(3382..4302)
                     /locus_tag="VFV99_11745"
     CDS             complement(3382..4302)
                     /locus_tag="VFV99_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5060028.1"
                     /translation="MKVRFWGVRGSIPSPGRATNRYGGNTSCVEVRPHGAAPIIIDAG
                     TGIRRLGKTLMEDSFRDGKGQCSILISHTHWDHVQGLPFFSPLYRAGNKIQIFARQRD
                     MHLEAVFSQQHNAPYFPVPLNAMQAEMSFHELIEGASFDIGKAKITCARLNHPWVAIA
                     YRVDVDRASVVYCSDTAPFTDMLLGRDFIERPSFGTLPPAVQDELTVMRSGIVALAKN
                     ADLLIYDTQFTPEEYKSRPHWGHSRPDDAIEIARAANVKKLCLFHHAPMRTDDDNDKI
                     LATYRELTQADPFELVSAYEGLEIPLGDGP"
     gene            complement(4344..4796)
                     /gene="dtd"
                     /locus_tag="VFV99_11750"
     CDS             complement(4344..4796)
                     /gene="dtd"
                     /locus_tag="VFV99_11750"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5060029.1"
                     /translation="MRAVVQRVSRASVTVDTRVTGAIEQGLLVLLGAGAGDGPTDLAY
                     IVDKIVNLRIFPDDAGKMNRSVLDVGGGVLVVSQFTLYGDARQGRRPAFTGALEPVAA
                     KTLYEQALAAILASGVKRVEAGEFAADMKVELVNDGPVTILLDSRKLF"
     gene            4865..5323
                     /locus_tag="VFV99_11755"
     CDS             4865..5323
                     /locus_tag="VFV99_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060030.1"
                     /translation="MRSVVLLALLFAFGCGKEIGDACVVSSDCSPNGDRQCDISQHEG
                     YCTIQGCDFSTCPDEAACIRFFTGVFQNKDCATTADCSLDELCSLTLKCVPRSSEIRF
                     CMKTCDTTDDCRDGYECRGIKEMKEHGGEPVLEGNAMPTESSPKFCAAAP"
     gene            5342..6277
                     /locus_tag="VFV99_11760"
     CDS             5342..6277
                     /locus_tag="VFV99_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5060031.1"
                     /translation="MSIGSFLLRRLGSGALAILGVSVLVFAFLHLVPGDPVDHLLGGE
                     ATADQRQKMEHCMGLDLPAHEQFVRFLGHVVDGSLGVQCPDPKGKPTVAARVFEVFPY
                     TIELAIAGMLVAIVFALPLGVIAAVKRSTWVDTFATVASLSVIGLPMMVLAPILLLVF
                     FIWLGWSPGPTETGLSALVLPAIAVGLHLMAMLARMTRSSMIEVLNEDYVRTARAKGL
                     SERRVLIHHALRNALLPVITVAGLQFGSLLSGAIVIEKVFARPGLGLTLLEAINERNY
                     SVVQGTVLVIAVIYVLVNTLVDLAYGFADPRIRRG"
     gene            6274..7107
                     /locus_tag="VFV99_11765"
     CDS             6274..7107
                     /locus_tag="VFV99_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5060032.1"
                     /translation="MKAALKLSLSGRVGLVMVGAFILVGVFGPLLAPYDPQAVDLNAR
                     FLSPSSEHWLGTDSNGVDTLSQLLWGARSALTISVIVVAITSTAGVMVGTIAGWFRGA
                     VDEVLMRIVDVLMAFPGILLNIAVVATVARPGLGVMIGALCLNGWVGYARVARGEVLA
                     LREREYISAAVAIGASHRRIMLRHLIPNILGPAFVQMAFAFGSVIMIEATLSFLGLGP
                     QLGYTWGAMLDQGTTFLWRAGFTHYTLLPGLAIMWVVLGANLLSDGLRDRFDPRQRGR
                     Q"
     gene            7107..7421
                     /locus_tag="VFV99_11770"
     CDS             7107..7421
                     /locus_tag="VFV99_11770"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5060033.1"
                     /translation="MALRVKVCHLSDVVDDEIRAFQVNGVTWPVIVTRVGGELYACPG
                     VCPHEDVALYNGELFGTTLRCPGHSYDFDLRTGTCKHDRDLELRRYPITLVGDEIWVD
                     LL"
     gene            complement(7426..8415)
                     /locus_tag="VFV99_11775"
     CDS             complement(7426..8415)
                     /locus_tag="VFV99_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5060034.1"
                     /translation="MAFDVRAATERDSAEVAQLIREMIPGVAADARLSWLYHQNPAGR
                     ALTWIASENGEVAGCTSYFPFRLQLDDTQRLCALGGDGYVRPKFRRRGLGALLHDAAR
                     DGMAAAGIGCMYGAPGAMNLTPLKHGGSREIGHVMRWVRPLRGAAFGIQAPFDQMIGG
                     VLRPRFVPELQPMAHGDARVDAVWRAFASTVPLAAVRDATFYTWRFLEAPAREQHPFV
                     IVDRGQPIGACALQRMPEGRALRIVDLLAVPGAWHRCLSAIARHAADTDAHIVDIKLM
                     ALDGRRRAMWRAGYVERDRKPFLVVIPKDGDRRFLDPMRWFYCGADSDLDVLD"
     gene            complement(8554..11319)
                     /locus_tag="VFV99_11780"
     CDS             complement(8554..11319)
                     /locus_tag="VFV99_11780"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5060035.1"
                     /translation="MSMTRVMATVLAAVFMVSCAPEAPKFAFKASERRGKLESNGLRF
                     VIMPDDSTQLAEVDIRYDVGAREDPQGRAGIAHLVEHMMFQTRPDGPNTPPIFQTILD
                     IATDMNAYTSWDQTHYRMTSRSENLDSMLKIEAMRMYYQCQNIPEQEFEREREVVRNE
                     IRAQSSAEGQLYQHVLAAMYPKGHAYERIVGGNDEQIASATLPEVCDFMKKYYTPDRA
                     VLLVAGDVNIDDTVNTIQKWFAKIPKAAAAPRTEVKPFTVEHKTVELEADVERPSVWF
                     GWPLPPRNTPEGEMAQFGVGRLEGELAEKAHDYDFAYNVGGGVLGGDLAPLFVIQVEL
                     KGMGKLDEAIEFAQKAAKQAYRGFDMANYEANESFRKRRQAGFVQSLERLVARTEQVA
                     SMIQFSKDIDFDSSDMYVFHELDKIAKFDIAKVSSVVKKYLDWNKAGIVVVKPGTNGF
                     GQDKRSKVKYAAKSDAAFVDPPVDPAEAKHPVRNAGQLKNLSGAQRFKLGNGMNVVML
                     PVHAMPLATAALIFNNAGDASTPNNPLLASSAAEFLSFPPDAEVFSQTGINVSCSTTD
                     DAMTCRTRGINIYLDVMLKGLERKIKAGTYSQQQIEDWQKSVKEQYKLKSTQEDTEYI
                     RQVVLALYGADHAYTKTAVLTPEAANKIHKDSLDDFRSKHYSAGNATLIIVGDFEPKY
                     AEKLARSTFGEWGTGSIDKPVDKTAAPRTGPVFVGTLKSKLDQQITATIAYPAPAGID
                     GQEAARQVLAGMLQLRAEGIRFKLGSTYGLSFARQPKVGPTAYMLRGGAVVGGTIDAE
                     RAGESIKALRESIDALRAGDHFDEDFVRARRKLLTQLLSESTVTSELAARLGVIATYG
                     LDANYYNTLVQQIAAVSPAQVKALMKAELDPAKEVVVLLGDKDHVNKTFAEAGIKDVK
                     LVEPEYK"
     gene            11488..13104
                     /locus_tag="VFV99_11785"
     CDS             11488..13104
                     /locus_tag="VFV99_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX5060036.1"
                     /translation="MSKDMDKAFFGHPAGLSTLFFTEMWERLSYYGARTFLAIYMVTP
                     VALGGRGMSDGATGLVMALYMSSVYLLSLPGGWIADRFMGQRKAVTVGGVGIALGNAM
                     LALPIDSLFLPGLAVIAFGTGFLKPNISTLVGQLYKPDDVRRNAGYTIYYMGINIGAG
                     AAPLVGMFIAQSPSFRAMLADHGIDPNMCWKIGFAVPAVGMVFGLIQYFIGYKKLGTA
                     GIQPTIPTDKVKANRDVTVLKTIAASLVVLIGGGILIDQFVYELSGELIGNIFSVGLL
                     IAAVAVFVGFFKGARDHNERTRVAAMLPLFIGAIAFFALFEQASTTMSLYAERIVDRH
                     LLGFYVPASFYQFWNAIFIVILAAPFAAMWLKLGKAGKEPTSVNKFGIGMIFAALSFC
                     VMLPTLAQAETAQCLTKEGCNLVSPYYLVVFYFVSTLSELFISPVGLSSMFGLAPQRL
                     AGMVMGTWFLATAIGNYIAGRAASISEGHGYGYLFKLLIVSALVIAAALFAVAPRIKR
                     MMANPKASAPADHSQKAEPDPLPAARTVED"
     gene            13104..13343
                     /locus_tag="VFV99_11790"
     CDS             13104..13343
                     /locus_tag="VFV99_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060037.1"
                     /translation="MRGRKPVPPVRDPDEALPDARDGLTRLERIILWQLREAQKETGR
                     ESIPAPMLYGRVVEHISVSVEEFQTVLTRLVGRGL"
     gene            13350..14603
                     /locus_tag="VFV99_11795"
     CDS             13350..14603
                     /locus_tag="VFV99_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060038.1"
                     /translation="MRFLLILLCACRVSWSTWQGAPPRSANNVTMTLYAVDESSMTMV
                     RSVLQRDPHITLVEFDPTVIEKQKTDCGSASCPAAVAAACSWSALQGAEYYALAAVGA
                     SFTSGYECTQYKGDLFAKHDECVAGHVTNEQTTASYTLDVYEVKTCEKVPALSQKMTA
                     HAQGPEEQSKPAALASLGAQVPAKSEGLPDQIAIDANGRIAGDARDGYYALFRDGHYR
                     GYVRARDPGTPAESLRPMYCCLSPQPGDALVARGRRKFLDLAVDGVIGQLTFDGERKI
                     AGGIGAHVRHYALDGGLQYGFGVDYLGSVATKSNIFLFTPEVGWGIPIAPGFVVSANL
                     GVGFARANQVIESMNDAVPWSLTGHVVPSARVQTFLTTWFYLTGDVGFAFTGTFDSWE
                     SDGAPAARPMTMRSPIVRLYAGFDI"
     gene            14697..15863
                     /locus_tag="VFV99_11800"
     CDS             14697..15863
                     /locus_tag="VFV99_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060039.1"
                     /translation="MRLLLLLVLFAGCRAGFYSWQVAPPNSAPKIKIALNAVDANASG
                     VARPVLQQIGRVQIFDVAPIVRDRLATECTNESCQLAIACQWSTQHGATYYARASVAA
                     TAGAYVLDVYDVNGCKRVPALSQELGGQAQPAHAEPEGQPPGQTPPVPEQPTIGKNGI
                     EGAPGDGYYALFRGNKYLGEVHVRDAGSPDEKVRAMHYGTKPMVGDLLDPRGIRRFVD
                     VALSAGAAWAFSEPMLGLGAHLRFYSFEGGLQVGFTGELLFHDDDFVGVFTGEVGYGW
                     PIAPGLILSGNLGAGTVTTHYYRPELEFPLGGRGSQLTPLVRLQTFFVGSYYATLDVG
                     VILTDDADKLDLPAAQIPFLRLSLGADVRSSASEKNSHARRAAAARATTPTSAR"
     gene            complement(15781..>17216)
                     /locus_tag="VFV99_11805"
     CDS             complement(15781..>17216)
                     /locus_tag="VFV99_11805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5060040.1"
                     /translation="QLLFATPFPLGVDGYFYPIQVRSILEHGTLQYPASPLTFYFMAP
                     FAAATDPIVGAKLGAALGGALIGLPAYAVGTRFGASRGAGLVAAAVATASASSAYLTI
                     EFVKQGVGLTVGLTALWLLLRALETPSRLRIGLAIGGLCAALLTHKLAAVTVIAIAIP
                     ATIEEARGRAKLRGRRLLYLLIALALLAVVALVLGLAFPQRFLSASDIALLGDTLTTS
                     AHWSAPALATEHLTLAFDHEAAIGAVVALAAAVFVWRRPRAAKGHHGERVAMWMVIGF
                     GVAIALPWLAVDDPQGLGFRLRIAGFVPLALNAGIVADALLMRIKRLTKHERDLAATG
                     LALFLAIRPHARPTEGMVLAHPAMVAAVMASAQQIPPGKTVIVPERHILFMVAWYARV
                     PVSLTPDRVPYADRVRMMPLAFIGMESPLDAALDRARNEPGVTPPVGFHPRHRNGLVL
                     VTEPTWEWLLAQLPPAARDYFSRWPTI"
BASE COUNT         2753 a         5652 c         5717 g         3094 t
ORIGIN      
        1 cctatccgat cgagagctgc gacgacgcta cggcggccac cttacctgag cggtgaacac
       61 agtcgcgtcg ccggggccta taaccgcacc acctgtcact acgaggtaat caccggcgac
      121 cacgagctcg tgattcgtgc gcgccatcgg caacgcgctc tcgcgccatg cgccgagcga
      181 gccgtcggcc gtggcatgcg ccgcgaacac ctgcgcggta cccggcgcgc cgaacaccgc
      241 cgcacggcca ccgacgacga acacccagtc atcgaccgcc actgcttcac cgaacccgac
      301 gggcacgggc agcggcgtcg tcgcgcgcgc aacgccgccc atcagccgcg cgacgaacac
      361 gttcgcgacc gcaccttgcg ccgggtcgtg atagccgccg agcgtgtaga cgttgtcggc
      421 actgaacgcg tactcggctt gcgcgaggaa gtcgatgccg agctcttcgg tctgccacgc
      481 gagcgagggc gtgatcggtg actgcagcgc caccgttgcg ttgccctccg ccggcagtcg
      541 cgtgtccatc agcacgagtg tgtcggcgtg cacggtcgct tccgacgaca ccacggtagc
      601 tggcagcatg ccgagcgatg ccaacgcgcc gaggtggccg gtctcgtcga gcgtcgcgct
      661 ccatacctga cggccgtgcg ccgggtcatc gtagatgccg tcgaggacgt acagcgtccg
      721 tccgtcgctg gtcgcgctgc actcggtggc cgggctcggt agcatgccgg ccagcctcca
      781 gttgccgagc acgccctctt cgatccgcgc ggcgtggatc tcggcggtct tcacgaaggc
      841 gccgctgtcc atgtagttgc cgccgaccac gagcacccag tcgtcgatca cggcactgca
      901 gtgattcgcg cgtggtgtcg gcagcgccgg tccgacctgc cacgggccga gctcgccggt
      961 tgcgacgtgc ggcggcggct cggtgcacgc aacaaaaagc aacaataggg cgatgcgcat
     1021 cgcgcgcacg ctctctcgca cctgcgcatc cgggcaagca tgactttgtt gcagctcgat
     1081 gacctgttgc tgcgcgagga cgatcgtgtg gcgcgcgagc aacggcacca cggggtcggt
     1141 cgcgcgaact cggtgaaatc gcgcgcgcga cactgggcaa ggcaattgct tgggtcgcca
     1201 agaatgcgcg cggccgtcct ctccgtggcg ctcactgcct gtgcgtaccg cccggggtcg
     1261 ttctcccatt tgtcggagag cttccccggg caacgcacga cggtgggctg catcgatctc
     1321 tccgtcgagc gtcgccccga tctgatcggc ggcggcaccg tgctcgccta cgcgttcggc
     1381 aatcgctgcg accatccggc gatcgtcgat ttcacgcagg tgaaggtaca tgcgcgcacg
     1441 tacgacgggc gcgacttgat tctcgccgcc ttcgatcccg aggggcagat ccaaccgctc
     1501 gagctcgacg gccgggccac cggcgaagaa gcgatcgcgt acaagatcga cgcctcgctc
     1561 gccgaggttt gcgtcgacgc tgcctcgctc acccacgaat cacccgcgcg ctggttgtgc
     1621 ttcgcgagca acctgccgcc ccagatcgcg gaggctggca aatgaaatac ctcctcttcg
     1681 cagcgctcct actcgccgat ccaaggccgg tgcacgccgg ttcgtgccac ggcggcggta
     1741 acgctggcac cagcagcagt ggaagccatg acgacggcgg ccgctcgtcg agctcgtcga
     1801 acgacacatc gtggtcatac acgccatcgt cggccggcga ttacgtcgtc cactcgagcg
     1861 aatgcatcga caacactgac gtccacggct ttcgtcactg ctcgaagttc ggcgcgtggg
     1921 gcaagaacat gcgcattccg aagctgttca tcgaggtcgg tggcaacgtg cgcaacttcg
     1981 gcagcgctct cgtcgatcgc accggcacga tctcgcacgg ctacgaatcg ttcgcgtatc
     2041 gcgtcgtgat gcccggcgcc gacgcgcctc gcgatgtcgc gatgacgtcg acgctacggc
     2101 tcggcttcgg cattccgcgt ggcttctata gcgggctcga attcgagctc ggcgcgctcg
     2161 tgtcaccggc ggcggcgact gccgagatga cgacggccgg cgcattcggt acgccaaacg
     2221 tcgagcagca gcacggcatg ttcctcgggt tcaccggcgt cggtggctat cgtgcggtgg
     2281 gatcgcgcgg cgcgatcgcg atcgagggcg cgggtggtct gcgcagcgtc cgctacaact
     2341 tcgacagctc gtatcattat tgcgagacga ccacgacagt taccgcaaac atgggcgtcg
     2401 tcgaagcgcg cgctcgtgcc gagctgtggc tcagcccgtg gctcacggcg ggtgtcaccg
     2461 tcggctcgaa cgtgctcgag cgcagcgact ggatggccgg tgtctacttc ggcgcccaca
     2521 gccgcgcgta cgcaggtgcg cgttagatct cgagctcgtc gccttcgcgc gcgggcacca
     2581 cagcgatgcc gtggggtgcg agcttggctt tgcacgcggc gacgatctgg tcgacgtcgt
     2641 cgtcggtgta gtcctgatcg tagtggaacg tgacgaggcg cttcgccttc gcggcgatcg
     2701 ccgcgtcgca cgcgtcgaca tacgaggaga agccgcggtt gcggcgcgtc gcctggtctt
     2761 cggggcggta cgtcgagtcg tggaggagga cgtcggcgcc cgagtagtgc tcgatcgctt
     2821 cgggcgttgg accgctctcc gggtaaccga cgtcggatgc gtagacgaac acgcggcccg
     2881 cttcctcgag ccggaacgcg agcgccgtcg tctggccgtg ctggtgctcg cgcgcgctga
     2941 tcgtcacgcc gtcggcttcg atcgtcttgc cgcacaccat cgcggagacg tcgaaggtcg
     3001 ccgcgaggtt ttttagcgtc tgcagcggcg agaagttggg atccatctgg ccttcgagga
     3061 tgtcctcgag catctcggag gagttgcctg ggccccacac gcggaactga ttgccgggga
     3121 tgaacacggg cgggaagaag ccgaggcctt ggatgtgatc ccagtgcgtg tgcgagagca
     3181 ggatcgtcgc gcggccttga cctttgccga atgtgctcgc gagcagcgcg ttgccgaggt
     3241 gcatgaggcc ggtgcccata tcgatgatga tgaggccgcc gtttgcggtc gtgaccgata
     3301 cgcacgacgt gttgccgccg tagcgattgg tttcggggcc gggagccgga atcgatccac
     3361 gtacgcccca gaatttgatc ttcatgggcc gtcccccagc gggatctcga gtccctcgta
     3421 ggcgctcacg agctcgaacg ggtcggcttg cgtgagctcg cgatacgtcg cgaggatctt
     3481 gtcgttgtcg tcgtcggtgc gcatcggcgc gtggtggaag aggcaaagct tcttcacatt
     3541 cgcggcgcgc gcgatctcga tcgcgtcgtc gggacgtgaa tggccccagt gcggtcgcga
     3601 cttgtattcc tcgggcgtga actgggtgtc gtagatgagc agatcggcgt tcttcgccag
     3661 cgcgacgata ccggagcgca tcacggttag ctcgtcctgc acggcgggcg gcagcgtgcc
     3721 gaagctcggg cgctcgatga agtcgcggcc gagcagcatg tctgtgaacg gtgcggtgtc
     3781 ggagcagtac acgaccgacg cgcgatcgac gtcgacgcgg tacgcgatcg cgacccacgg
     3841 gtgattgaga cgcgcgcacg tgatcttcgc cttgccgata tcgaacgatg cgccctcgat
     3901 cagctcgtgg aagctcatct cggcctgcat cgcgttgagc gggacgggga agtacggcgc
     3961 gttgtgctgc tggctgaaga ccgcctcgag atgcatgtcg cgctggcggg cgaagatctg
     4021 gatcttgttg ccggcgcgat agagcggcga gaagaacggc aggccttgga cgtggtccca
     4081 gtgcgtgtgg ctgatcagga tcgagcactg acctttgccg tcacggaacg aatcctccat
     4141 gagcgtcttg cccagacgac gaatgccggt gcccgcgtcg atgatgatcg gcgcggcgcc
     4201 atgggggcgc acctcgacgc acgacgtgtt accgccgtat cgattggttg ctcgcccggg
     4261 cgatgggatc gagccgcgga ctccccagaa cctgaccttc atcgggcgcc accatgaacc
     4321 taacgcgccg agatgcctaa ccgctagaac agcttgcggg aatctagaag gatcgtgacg
     4381 gggccatcgt tgaccagctc gaccttcatg tccgctgcga attccccggc ctcgacgcgt
     4441 ttgacacctg acgccaaaat ggccgcgagc gcctgctcgt agagcgtttt tgcggccacc
     4501 ggctcgaggg cacccgtgaa ggcgggccgc cggccctgcc gggcgtcgcc gtagagggtg
     4561 aattgcgaca cgacgaggac gccgccaccg acgtcgagca ccgaccgatt catcttgccg
     4621 gcgtcgtcgg ggaagatgcg caggttgacg atcttgtcga cgatataggc gagatcggta
     4681 gggccatcgc ccgctcccgc accgagcagg accagcaatc cttgctcgat tgcacccgtg
     4741 acccgggtat ccaccgtcac gcttgcccgc gaaacccgct gaactacagc gcgcatggcc
     4801 tggttataca ggcaactagg ttgccttgcc ggaggtccaa ccggccgcta ggatgcgccc
     4861 gctcatgagg tctgtcgtct tgctcgcgct cttgttcgcc ttcgggtgcg gcaaagaaat
     4921 cggtgacgcc tgcgtcgtca gctctgactg cagcccgaac ggcgatcgcc agtgcgacat
     4981 ctcgcagcac gagggctact gcacgatcca gggttgcgac ttcagcacgt gccccgacga
     5041 ggcagcctgc atccggtttt ttaccggcgt gttccagaac aaggattgcg cgacaaccgc
     5101 ggactgctcg ctcgacgagc tgtgcagtct cacgctcaag tgcgtgccgc gtagctccga
     5161 aattcgcttc tgcatgaaga cgtgtgacac gaccgacgac tgccgcgacg gatacgagtg
     5221 ccgcggcatc aaggaaatga aagagcacgg cggcgagccg gtcctcgagg gcaacgccat
     5281 gccaaccgag agctcgccga agttctgcgc cgcggcgccc tgagtagtac gctccagcgc
     5341 agtgtcgatc ggctcgttct tgctccggcg cctcggctcg ggtgcgctcg cgatcttggg
     5401 cgtgtcggtg ctcgtgttcg cgttcttgca cctcgtcccc ggtgatccgg tcgatcactt
     5461 gctcggcggc gaagcgaccg ctgaccagcg ccagaagatg gagcactgca tggggctcga
     5521 tctgccggca cacgagcagt tcgtgcgctt cctcggccac gtcgtcgacg gatcgctcgg
     5581 cgtgcagtgt ccggatccga agggaaagcc gacggtcgcg gcgcgcgtct tcgaggtgtt
     5641 cccgtacacg atcgagctcg cgatcgccgg catgctcgtc gcgatcgtgt tcgcgttgcc
     5701 gctcggtgtg atcgccgcgg tgaaacgcag cacctgggtc gatacgttcg cgaccgtggc
     5761 gtcgctgtcc gtgatcgggc tgccgatgat ggtgctcgcg ccgatcttgc tgctcgtgtt
     5821 cttcatctgg ctgggctggt cgccgggacc gacggagacc gggctgtcgg cgctcgtgct
     5881 gccggcgatc gcggttggct tgcacctgat ggcgatgctc gcgcgcatga cgcggtcgtc
     5941 gatgatcgag gtgctcaacg aggactacgt acgcaccgcg cgcgcgaagg ggctgtcgga
     6001 acgtcgcgtg ctgatccacc acgcgctgcg caacgcgctg ttgccggtga tcaccgttgc
     6061 cggtctgcag ttcggctcgc tgctctcggg tgcgatcgtg atcgagaagg tgttcgcgcg
     6121 cccgggcctg ggcctcacgc tgctcgaggc gatcaacgag cgcaactact ccgtcgtgca
     6181 gggcaccgtg ctcgtcatcg ccgtgatcta cgtgctcgtc aacacgctgg tcgacctcgc
     6241 ctatggcttc gccgacccga ggatccggcg cggatgaagg cggcactcaa actctcgctg
     6301 tcgggacgcg ttggcctcgt catggtcggc gcgttcatcc tcgtcggcgt gtttgggcca
     6361 ttgctcgcgc cgtatgatcc gcaggcggtc gatctcaatg cgcggttctt gtcaccgtcg
     6421 agcgagcact ggctcggcac cgactcgaac ggcgtcgata cgctatcgca gctactgtgg
     6481 ggagcgcgct cggcgctcac gatcagcgtc atcgtcgtcg cgatcacgtc gacggccggg
     6541 gtcatggtcg gcacgatcgc aggctggttc cgcggcgccg tcgacgaagt gctgatgcgc
     6601 atcgtcgacg tgttgatggc gttcccgggc atccttctca acatcgcggt cgtcgcgacg
     6661 gtcgcgcggc ccggcctcgg cgtgatgatc ggcgcgctgt gcctcaacgg ctgggtcggt
     6721 tacgcccggg tcgcgcgtgg cgaggtgctc gcgttgcggg agcgcgagta catctcggcg
     6781 gcagtcgcga tcggcgcctc gcatcgccgg atcatgctcc gccacctgat tccgaacatc
     6841 ctgggtccgg cgttcgtcca gatggcgttc gcgttcggca gcgtgatcat gatcgaagcg
     6901 acgctgtcgt tcctgggcct cgggccgcag ctcggctaca cctggggagc gatgctcgat
     6961 cagggcacga cctttctctg gcgcgccggc ttcacgcact acacgctgct gccgggcctc
     7021 gcgatcatgt gggtcgtgct cggcgcaaac ctcctctccg acggactgcg cgatcgcttc
     7081 gatccgcgtc agcgtgggag gcagtagtgg cgctgcgcgt gaaggtctgc cacctcagcg
     7141 atgtcgtcga cgacgagatc cgcgcgttcc aggtcaacgg cgtgacgtgg ccggtgatcg
     7201 tcacgcgcgt cggcggcgag ctgtatgcgt gcccgggcgt gtgtccgcac gaggacgtcg
     7261 cgctctacaa cggtgagctg ttcggcacga cgctccgctg tcctgggcat tcgtacgatt
     7321 tcgatctgcg caccggcacg tgcaagcacg atcgagatct ggaattgcgc cgctatccga
     7381 tcacgctcgt cggcgacgag atctgggtcg atctgctcta gcgattcaat cgagcacatc
     7441 gagatccgaa tcggcgccac aatagaacca gcgcatcgga tcgaggaacc ggcgatcgcc
     7501 gtccttggga atcacgacga ggaatggctt gcgatcgcgc tcgacgtagc cggcgcgcca
     7561 catcgcacga cgcctgccat cgagcgccat cagcttgatg tcgacgatgt gtgcgtccgt
     7621 gtccgccgcg tgccgcgcga tcgcggacag acaccgatgc cacgcacccg gcacggcgag
     7681 cagatcgacg atgcgcagcg cgcgcccctc gggcatccgc tggagtgcgc acgcaccgat
     7741 cggctgacca cggtcgacga tcacgaacgg gtgttgctcg cgcgcgggtg cctcgagaaa
     7801 gcgccacgtg tagaacgtcg catcgcggac cgccgccagc ggcacggtcg atgcgaatgc
     7861 gcgccacacg gcgtccacgc gcgcatcacc gtgcgccatc ggttggagct ccggcacgaa
     7921 ccgcggccgc aacacgccgc cgatcatctg atcgaacggc gcttggatgc cgaacgcggc
     7981 gccgcgcaac ggacgtaccc accgcatgac gtggccgatc tcgcgcgagc cgccgtgctt
     8041 gagcggcgtc aggttcatcg cgccgggcgc gccgtacatg cagccgatac cggccgctgc
     8101 catgccatcg cgcgcggcat cgtgaagcag tgcgccgagt ccgcggcggc gaaacttcgg
     8161 tcgcacgtag ccgtcgccgc cgagcgcaca gagccgctgc gtgtcgtcga gctgcaggcg
     8221 aaatggaaag tacgacgtgc aaccggcgac ctcgccgttc tcgctcgcga tccacgtgag
     8281 cgcgcggccg gcggggtttt ggtggtagag ccacgagagc cgcgcgtcgg ccgcgacgcc
     8341 gggaatcatt tcccggatca gctgcgcgac ctcggcgctg tctcgctcgg tggctgcgcg
     8401 gacatcaaac gccatgttgc agcgtacgcg ggaatcggta gcggggctcc cgcaacgctg
     8461 ctctgctcgt cggagggctg tggcgcgcac gcacggcgag cgctcgcgca tcgtcgaatg
     8521 cgcgcgaccg cgacgttaag cgcgtctacc gcgctacttg tactcgggct cgacgagctt
     8581 cacgtctttg atgcccgctt ccgcgaacgt cttgttgacg tgatccttgt cgccgagcag
     8641 tacgacgact tccttcgccg gatcgagctc cgctttcatc agcgccttca cctgcgcggg
     8701 cgacaccgcc gcgatctgct ggaccagcgt gttgtagtaa ttcgcgtcga ggccgtacgt
     8761 cgcgatcaca ccgaggcgcg cggcgagctc gctggtcacc gtcgactcgc tcagcaattg
     8821 cgtcagcagc ttgcggcgcg cacgcacgaa gtcctcgtcg aagtgatcgc ccgcgcggag
     8881 cgcatcgatg ctctcgcgca gtgccttgat cgactcgccc gcgcgttccg cgtcgatcgt
     8941 gccgccaacg accgcgccgc cgcgcaacat gtacgcggtc ggaccgacct tcggctggcg
     9001 cgcgaacgac agtccgtacg tcgagccgag cttgaaccgg atgccctcgg cgcgaagctg
     9061 cagcatgccg gcgagcacct ggcgcgcggc ctcttggccg tcgatgccag ccggtgccgg
     9121 atacgcgatc gtcgcggtga tctgctgatc gagcttcgac ttgagcgtcc cgacgaacac
     9181 cggacccgtg cgcggcgctg cagtcttgtc gaccggcttg tcgatcgagc cggtgcccca
     9241 ctcgccgaac gtgctgcgcg cgagcttctc ggcgtacttc ggctcgaagt cgccgacgat
     9301 gatcagcgtc gcgttgccgg ccgagtaatg cttcgagcgg aagtcgtcga gcgagtcctt
     9361 gtggatcttg ttcgcggcct cgggcgtcag cacggcggtc ttcgtgtacg cgtgatcggc
     9421 accgtagagc gcgagcacca cctgacggat gtactcggtg tcctcctgcg tgctcttgag
     9481 cttgtattgc tccttgacgg acttctgcca gtcctcgatc tgctgctgcg agtacgtgcc
     9541 cgccttgatc ttgcgctcga ggcctttgag catgacgtcg agatagatgt tgatgccacg
     9601 cgtgcggcac gtcatcgcgt catcggtcgt gctgcagctg acgttgatgc cggtctgcga
     9661 gaacacctcg gcatccgggg ggaagctgag gaactccgcg gccgacgacg cgagcaacgg
     9721 attgttcggc gtcgacgcat ccccggcgtt gttgaagatc agcgcggcgg tcgcgagcgg
     9781 catcgcatgc acgggcagca tcacgacgtt catgccgttg ccgagcttga agcgctgtgc
     9841 gccggagagg ttcttcagct gacccgcatt gcggactgga tgcttcgcct ccgccggatc
     9901 gaccggcgga tcgacgaacg ctgcatcgct cttcgccgcg tatttcacct tcgaacgttt
     9961 gtcctgaccg aagccgttcg taccgggctt gacgacgacg atgccggcct tgttccagtc
    10021 gagatacttc ttcacgaccg agctgacctt cgcgatgtcg aacttcgcga tcttgtcgag
    10081 ctcgtggaac acgtacatgt cactcgaatc gaagtcgatg tccttcgaga actggatcat
    10141 cgacgcgacc tgctcggtgc gcgcaacgag gcgctcgagc gactgcacga acccggcttg
    10201 gcggcgcttg cggaacgact cgttggcctc gtagttggcc atatcgaagc cgcggtacgc
    10261 ctgcttcgcg gctttctgcg cgaactcgat cgcctcgtcg agcttgccca tgcccttgag
    10321 ctcgacctgg atgacaaaca gcggcgcgag atcgcctccg agcacgccgc cgccgacgtt
    10381 gtacgcgaag tcgtagtcat gcgccttctc ggcgagctcg ccttccaagc ggccgacgcc
    10441 gaactgcgcc atctcgcctt cgggggtgtt gcgcggcggc agcggccagc cgaaccacac
    10501 gctcggacgc tcgacgtccg cctcgagctc gaccgtcttg tgctcgacgg tgaacggctt
    10561 gacctcggtg cgcggcgcgg ccgctgcctt cgggatcttc gcaaaccact tctggatcgt
    10621 gttgaccgtg tcgtcgatgt tgacgtcgcc tgcaacgagc agcaccgcgc gatccggcgt
    10681 gtagtacttc ttcatgaagt cgcagacctc agggagcgtt gcgctcgcga tctgctcgtc
    10741 gttgccgccc acaatgcgct cgtacgcatg gcccttcgga tacatcgcgg ccaggacgtg
    10801 ctgatagagc tgtccttccg cactcgactg cgcgcgaatt tcgttgcgga cgacttcgcg
    10861 ctcgcgctcg aactcctgct ccgggatgtt ctggcactgg tagtacatgc gcatcgcctc
    10921 gatcttgagc atcgaatcga ggttctcgga gcgcgacgtc atgcggtagt gcgtctgatc
    10981 ccaggacgtg tacgcgttca tgtccgtcgc gatgtcgagg atcgtctgga agatcggcgg
    11041 cgtattcgga ccgtcgggac gcgtctggaa catcatgtgc tcgacgaggt gcgcgatgcc
    11101 ggcgcggccc tgcggatcct cgcgcgcgcc gacgtcgtaa cgaatgtcca cctctgcgag
    11161 ctgcgtgctg tcgtcgggca tgatcacgaa tcgcagacca ttcgattcga gcttgccgcg
    11221 tcgctctgaa gccttgaacg caaacttcgg cgcctctggt gcacaagaaa ccatgaacac
    11281 tgcggcgagc accgtcgcca tcacccgggt catcgacatg cgcgcgatct ttgccaaatc
    11341 gcacccgatt gcaagactgt cgtcgtcggg ctgcagcctc accgaaaatg acctagcgcg
    11401 acgtgtcggc atatcgcccg cgacaccccc gtgtcgcagc ccgtttcgga tgactttggc
    11461 caggggcgat gggataagtt gcgcgcaatg tcaaaggaca tggacaaggc gttcttcggc
    11521 cacccggctg gcctatcaac gctgttcttc acggagatgt gggagcgcct gtcgtactac
    11581 ggcgcgcgca cgttcctagc gatctatatg gtgaccccgg tcgcgctcgg cggccgtggc
    11641 atgagcgatg gcgcgacggg cctcgtcatg gcgctctaca tgtcgtcggt ctatctgttg
    11701 tcgctgcccg gcggctggat cgcggaccgc ttcatgggcc agcgcaaagc ggtgacggtc
    11761 ggcggtgttg gcattgccct cggtaacgcg atgctcgcgc tgccgatcga ttcgctgttc
    11821 ttgcccggcc tcgcggtgat cgcgtttggc accggcttcc tcaaaccgaa catctcgacg
    11881 ctcgtcggcc agctctacaa gcccgacgat gtccgtcgca acgccggcta caccatctat
    11941 tacatgggca tcaacatcgg tgccggcgcg gcgccgctcg ttggcatgtt catcgcgcag
    12001 agcccgagct tccgcgcgat gctcgccgat cacggcatcg atccgaacat gtgctggaag
    12061 attggctttg ccgtgccggc ggtcggcatg gtgttcggtc tgatccagta cttcatcggc
    12121 tacaagaagc ttggcactgc gggcatccag ccgacgatcc cgaccgacaa ggtcaaggcg
    12181 aatcgcgacg tgaccgtgct caagacgatc gccgcgtcac tcgtcgtgct gatcggcggc
    12241 ggtatcctga tcgatcagtt cgtctacgag ctgtcgggcg agctgatcgg caacatcttc
    12301 agcgtcggcc tgttgatcgc cgcggtcgcg gtgttcgtcg gcttcttcaa gggcgcacgc
    12361 gatcacaacg agcgcacccg cgtcgctgcg atgctgccgc tgttcattgg cgcgatcgca
    12421 ttcttcgcgc tgttcgagca agcgtcgacc acgatgtcgc tctacgccga gcgcatcgtc
    12481 gatcgtcacc tgctcggctt ctacgtgccg gccagcttct accagttctg gaacgcgatc
    12541 ttcatcgtga tcctcgcggc gccgttcgcc gcgatgtggc tcaagctcgg caaggccggc
    12601 aaggagccga cgagcgtcaa caagttcggc atcggcatga tcttcgccgc cctgtcgttc
    12661 tgcgtgatgc tgccgacgct cgcgcaggca gagactgcac agtgtctgac gaaggaaggc
    12721 tgcaatctcg tcagcccgta ctacctcgtc gtcttctact tcgtgtcgac gctgtcggag
    12781 ctgttcatct cgccagtcgg tctgtcgagc atgttcggcc tcgcgccgca gcgcctcgcc
    12841 ggcatggtga tgggcacgtg gttcctcgcg accgcgatcg gtaactacat cgcaggccgc
    12901 gcggcgagca tctcggaagg ccacggttac ggctacctgt tcaagctgct catcgtctcg
    12961 gcgctcgtga tcgccgccgc gctgtttgca gtcgcgccgc gcatcaagcg catgatggcg
    13021 aacccgaagg cgagcgcacc ggccgaccac agtcagaagg cggagcccga tccgctgccg
    13081 gccgcgcgca ccgtcgagga ctgatgcgtg ggcgcaagcc cgtgccgccg gtccgcgatc
    13141 cggacgaagc gctccccgat gctcgagatg gtctgacccg gctcgagcgg atcatcttgt
    13201 ggcagctgcg cgaggcgcag aaagagaccg gtcgcgagtc gattccggcc ccgatgctgt
    13261 acggccgcgt cgtcgagcac atcagcgtct cggtcgaaga attccagact gtgctgactc
    13321 ggctggttgg ccgcggttta taacgaggcg tgcgtttcct cctgatcttg ctgtgcgcgt
    13381 gtcgagtcag ctggagcacg tggcagggcg cgccgccgcg cagcgcgaac aacgtcacga
    13441 tgacgctcta tgcggtcgac gaaagcagca tgacgatggt ccgctcggtg ctgcagcgcg
    13501 atccacacat cacgctggtc gaattcgatc cgacggtgat cgagaagcag aagaccgact
    13561 gcggcagcgc gagctgtccg gccgccgtcg cggcagcgtg ctcgtggtcc gcgttgcagg
    13621 gcgccgagta ctacgcactc gcggctgtcg gcgcgagctt cacgtcgggc tacgagtgca
    13681 cgcagtacaa aggtgatctg ttcgcgaagc acgacgagtg cgtcgccggc cacgtgacga
    13741 acgagcagac cacggcgtcg tacacgctcg acgtctacga ggtgaagacg tgcgagaagg
    13801 tcccggcgct gtcgcagaag atgacggcgc acgcacaagg acccgaagag cagagcaagc
    13861 ccgcggcgct cgcgagcctc ggtgcgcagg tgcccgcgaa atcggaaggc ctcccggatc
    13921 agatcgccat cgatgcgaac ggccggatcg ccggcgacgc gcgcgacggc tactacgcgc
    13981 tgttccgcga cggtcactat cgcggttacg tgcgcgcacg tgaccccggc acgcctgccg
    14041 aatcgctgcg gccgatgtat tgctgcctgt cgccgcagcc cggggacgcg cttgtcgcgc
    14101 gcggtcggcg caagttcctc gacctcgcgg tcgatggcgt catcggccag ctgacctttg
    14161 atggcgagcg gaagatcgcc ggcggtatcg gcgcgcacgt gcgccattac gcgctcgacg
    14221 gcggtttgca gtatggcttc ggcgtcgatt acctcggcag cgtcgcgacg aagtcgaaca
    14281 tcttcttgtt cacgcctgag gtgggctggg gcattccgat cgcgccgggc ttcgtcgtgt
    14341 cggcgaacct cggcgtcggc tttgcgcgcg cgaaccaggt gatcgagagc atgaacgacg
    14401 cggtgccgtg gtcgctcacg ggccacgtgg tgccgagtgc gcgcgtgcag acgttcttga
    14461 cgacctggtt ctatctcacc ggtgatgtcg gcttcgcgtt caccggcacg ttcgacagct
    14521 gggaaagcga cggcgccccg gcggcacgac cgatgacgat gcgttcgccg atcgtgcggc
    14581 tctacgccgg cttcgacatc tgatcgatca gcgcgacgat acgcgcgtgc tctgtagcac
    14641 gcccggcgca accgagtgcg gcggccgtcg cgtgcgcgta gtacaacgct ctcgccgtgc
    14701 gactgctgtt gctcctcgtc ctctttgccg gttgccgcgc cggcttctac tcctggcagg
    14761 tcgcaccgcc gaattcggca ccgaagatca agatcgcgct gaatgccgtc gacgcgaatg
    14821 cgagcggcgt cgcgcgcccg gtgctccagc agatcggccg cgtccaaatc ttcgatgtcg
    14881 caccgatcgt gcgcgaccgc ctggcgaccg agtgcacgaa cgagagctgt cagctcgcga
    14941 tcgcgtgcca gtggtcgacg caacacggcg ccacgtacta cgcgcgcgcg tcggtcgctg
    15001 cgacggcagg agcgtacgtg ctcgacgtct acgacgtgaa cggctgcaag cgcgttcccg
    15061 cgctatcgca ggagctcggt ggacaggcgc aaccagcgca cgccgagccc gaaggtcaac
    15121 caccgggaca gacgccgccg gttcccgagc agccgacgat cggcaagaac ggcatcgaag
    15181 gcgctcccgg tgacggttac tacgcgctgt ttcgcggcaa caagtacctc ggcgaggtgc
    15241 acgtgcgcga cgccgggagc cccgacgaga aggtccgcgc aatgcactac gggaccaagc
    15301 cgatggttgg cgacttgctc gacccgcgcg gcattcgtag gttcgtcgac gtcgcgctgt
    15361 cggccggcgc ggcgtgggca tttagcgaac cgatgctcgg tttgggtgcg cacctccgct
    15421 tctacagctt tgaaggtggc cttcaggtcg gcttcacggg cgagcttctc ttccacgatg
    15481 acgactttgt gggcgtgttc accggcgagg tcggctacgg gtggcccatc gcgccagggc
    15541 tcatcctgtc gggcaatctc ggcgctggaa ccgtgacgac gcactactac cgtcccgagc
    15601 tcgagtttcc gctcggcggg aggggttcgc agctcacgcc gctcgtccgg ctccagacgt
    15661 tcttcgtcgg gtcgtactac gcgacgctgg acgttggcgt catcctcacc gacgacgccg
    15721 acaagttgga tttacccgcg gcgcagatcc cgttcctgcg gctctcgctc ggtgccgacg
    15781 tcagatcgtc ggccagcgag aaaaatagtc acgcgcggcg ggcggcagct gcgcgagcaa
    15841 ccactcccac gtcggctcgg taaccagcac gagcccgttg cggtggcgcg gatgaaagcc
    15901 gactggcggc gtcacgccgg gctcgttgcg cgcgcgatcg agcgcagcat cgagcggcga
    15961 ttccatgccg atgaaggcga gcggcatcat ccgcacgcgg tcggcgtatg gcacgcgatc
    16021 gggcgtgagg ctgaccggca cgcgcgcgta ccacgcgacc atgaacaaga tgtggcgctc
    16081 gggaacgatg acggtcttgc cgggtggaat ctgctgcgct gacgccatca ccgccgcgac
    16141 catcgcggga tgcgcgagca ccatgccttc cgtcggtctc gcgtgcggcc ggatcgcgag
    16201 gaacagcgcc aggcccgtcg ctgcgagatc gcgctcgtgc ttcgtgagcc gcttgatccg
    16261 catgagcagc gcgtcggcga cgatgccggc gttcagcgcg agcggcacga acccggcgat
    16321 gcggagccgg aacccgagac cctgcggatc gtcgacggcg agccagggca gggcgatcgc
    16381 gacaccaaag ccgatcacca tccacatcgc gacgcgctcg ccatggtgtc ccttcgcggc
    16441 gcgcggccgc cgccatacga agaccgcggc ggcaagcgcg acgaccgcac cgatcgctgc
    16501 ttcgtgatcg aacgcgagcg tgaggtgctc ggtcgcgagt gcgggcgccg accagtgcgc
    16561 gctggtggtc agtgtgtcgc cgagcagcgc gatgtcgctc gccgacagga agcgttgcgg
    16621 gaacgcgagg ccgaggacga gcgcgacgac cgcgagcagg gcgagcgcga tcaacagata
    16681 gagcagacgg cggccgcgga gcttcgcgcg accgcgtgct tcttcgatcg tcgccggaat
    16741 cgcgatcgcg atcaccgtca cggcggcaag cttgtgtgtg agcagcgcgg cgcagagccc
    16801 gccgatcgcg aggccgatgc gcagtcgcga cggcgtctcg agcgcgcgca agagcagcca
    16861 cagcgcggtg agcccgaccg tgagcccgac gccttgcttg acgaactcga tcgtcaagta
    16921 cgccgagctc gcgcttgcgg tcgcgacggc cgccgcgacg agccctgcgc cacgactcgc
    16981 gccgaatcgc gtgccgaccg cgtaggccgg gagcccgatc aacgcgccgc cgagcgccgc
    17041 ccccagcttc gcgccgacga tcggatcggt cgccgccgcg aacggcgcca tgaagtagaa
    17101 cgtcagcggc gaggccgggt actgcagcgt cccgtgctcg agaatcgagc ggacctggat
    17161 cggatagaag tagccgtcga cgccgagcgg gaacggcgtc gcgaacaaca gctggc
//
LOCUS       DASUFX010000301         9126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1714005, whole genome shotgun sequence.
ACCESSION   DASUFX010000301 DASUFX010000000
VERSION     DASUFX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714005"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..484)
                     /locus_tag="VFV99_11810"
     CDS             complement(83..484)
                     /locus_tag="VFV99_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5060041.1"
                     /translation="MKRWLLALAACGASTPPPTPVDKTVQLNTDGALVHVEDSLVDGY
                     VTIVDFWSTSCEACTIVTGMIAVQIAKQPQVIVRKVDVGDGFTAVAKAYDIGTLPHVN
                     VYDRKRRLRYRLIGPEALRAPTLARELLAEP"
     gene            complement(481..2046)
                     /locus_tag="VFV99_11815"
     CDS             complement(481..2046)
                     /locus_tag="VFV99_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5060042.1"
                     /translation="MKGRAVGIDLGTTNSVVAVIDRDGAPRILTTEEGSTMLPSLVWF
                     SPQGPIVGEAAREGLEHSPDLTIFGAKRLLGRRFDHPEVRKLARVLPYELTEAPNGDT
                     WIQLAAGRAVSPEEVSALILRELKRTAERFFREPVTRAVITVPAWYDAAQRQATKDAA
                     QIAGLQVMRLLSEPTAAALGHGAHRGADRRYLVCDLGGGTFDVALVDVDAGVFEVLAT
                     TGDPFLGGDDVDRVLVENLVRDVRQSRGIDVSTDASAIEKLRLAAQLAKHELSDHATA
                     KLQIPDLAQLPSGKHLEYVRMLRRDELELWTSPLVKRLEPPVKESLLRSSRQRDAIEE
                     VLLVGGSTRMPVVRKELARMTGREPSVIANPEEVVAVGAALEVARLDGTIEGVLLIDV
                     AARGLMMSRQGQDCEPVIAQSSVIPTREHKLLPTRHDDQVRVEFDLWEGESADPSANR
                     HLGRYAVVDLPEAPGGDVLVLVEVTIDTDGVLRLGASELVSGDRVMIEQVYHAGLSRT
                     DVARLVKQLAESQ"
     gene            complement(2043..3194)
                     /locus_tag="VFV99_11820"
     CDS             complement(2043..3194)
                     /locus_tag="VFV99_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5060043.1"
                     /translation="MASRTLQVRCSSWEQVDIFTTRKLRKGKLLSMKVPFSAKTGMQV
                     TIGLELPNEVVVAIEGVVQKSAPVEVDGKVDKSRTWIEIELTGFTDEVKARLKRLQRD
                     AEDPETDPNIAAAHLVTPAPPVRRSQSQIGGDDLPADERELFQHLTAELRRLRQAAVH
                     DVLGVPPNAGPELVRASWKDLIRRNHPDLVARRNAPAITHLAEELTILANRAYDRMRT
                     SLVAEGRAATAGPTVASPAGWLVAFEDIKSDPANATPPKGSLRRFERPPTPQPVPPPP
                     PPSAASGQGSEAYEGRARAMLALGDANAAREVLAAALVVYPRSKPLRSLYYVATALAA
                     VLDGELMLATAQLETALAHHEQCSEASKMLEHIRRHGTAEADAMRRLFR"
     gene            complement(3194..5362)
                     /locus_tag="VFV99_11825"
     CDS             complement(3194..5362)
                     /locus_tag="VFV99_11825"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060044.1"
                     /translation="MAGQAAEQIIAGVVLSERLAVTMYGAIHRASFGGQRNLRGLVVD
                     PRMLEEDAFRISLTDAKNIAKLTKLEHPNIVPTMTVESGGPDVVIVTRGVGRYVTVQD
                     LITSAKARGKQGGKLSTPVAGQIGKSVIEALAAAHRAGVIHGAVHPRSVLIGEDGNVL
                     LADFIAGLALTTAVAQGADSSLWRGLTGYIAPELVVGEDPTPGVDVFAVGAMMFTMLS
                     GEVPPGSLRVTPAVERLVQRALDTDPTRRYKTASDMLENLIEAMEDDRWELADRGELI
                     KEAGLSATDENIDDATEDLLASLGKSEVKVTTPPRKDVEALGAKHQRANSTQNRLDAL
                     LADLGGGDDTGLTQVDDHPAGAAKRRDPISEVIRNERRMPVPRVPSLDDPDDEEDDAT
                     PLPPPSRGTTSADEAAALDAINDLDASGARRASSQKIEQAAKRVESEQRKPEQRKPAP
                     RAQPIVDPIRDDDMPTPRLKSPLRSAVAGLLVLGVLGGAGYVVYTKFQEDKAREAQAK
                     EEREKQLKEKDDIEKQLREAQADSGALDITSSGAGIWLKLGKTPLDTTIKLHADQAHD
                     MVLLHSGNEVTEAQVNGTSWSGAKEALKAKLTVTMKPSKAKAEPVLPLQPTTPVLGST
                     GVVGAGIVHIESTPADAEVWLFIGANHAQFNELIAGRDYEAAVVKPGFKTQHVMFKAD
                     EWRDGGDPSTPIDVAKKKPMLSRTVELEPDPDYKPPKKGK"
     gene            complement(5373..6209)
                     /locus_tag="VFV99_11830"
     CDS             complement(5373..6209)
                     /locus_tag="VFV99_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5060045.1"
                     /translation="MSELQPLATSIGGLVVAVVLGLVWGSFANVCIYRWPPTEEFPKG
                     RSVVKPDSHCFACKAPIRWYDNVPLFSYLWLRGRCRNCKAEFSPRYVLVEAVTGALFG
                     CAWWFTLGTGGLFQPFDTRLLHFVVYAAFVWVMVVITFIDIDHKLILNKVTIPAIVAF
                     YAASLAFRPWWEGLVGIAIGYGLPWLIGELYLRIRKREGLGLGDATMLALIGALFGWR
                     GVVVSLFGGSVVGSIIGIAVLVRKKQQIMQSELPFGPFLAIAAVFYLFAEPFLNVYFR
                     LL"
     gene            6326..7258
                     /locus_tag="VFV99_11835"
     CDS             6326..7258
                     /locus_tag="VFV99_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060046.1"
                     /translation="MPDEQLGGLVIAPKTKAAPIDVTRAAKDPRELGRALSLPYGDVI
                     TALGPHTYAITTSTVVEEGGKKVNELSDQTKIDLGDNAAFSAVYTNSADYGREAISIG
                     GQLYLRPRYQRWHGRAPEMPDEPAQIRDSFFVPLAATWELFAPGAELSDLGTAQVAGR
                     SGKKIAVKLSPNGAKPAAETVSQRKWREKRSIESLSGEIVIDADKGVPLSVKLAGTIG
                     FSRDGRHFTMKVSLEASATAFGKVAIAAPPADQVVATPERLREVDDRDFLLQGIAPPL
                     RRNPDGTAVTPQPKMAGPGSVTPQPKEAGSNSGK"
     gene            7255..8049
                     /locus_tag="VFV99_11840"
     CDS             7255..8049
                     /locus_tag="VFV99_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060047.1"
                     /translation="MTALVQRRDYLVGQRRTIIARSLMGSLAGALPIPFLDDWAVGRL
                     LGGGYRKIAAAHQVDMTDEAVDNMVHGPTKPPPIAQMAIGGVLSRVASKAARRMMLAI
                     ATVNRARSAARTFTAMTLFDHYCAKLHTGFGLDGATALALREEIIRTIDQTPGALAFH
                     PFRRGALSAARATLKAPLELADLASGGRLRKLLSNKSEITTPQTVDEVEQAMESVLAD
                     KTTFLSRTVAAVEVQLSAEVNPYLDDVLESLDRRWRARVAAKADLK"
     gene            8046..>9126
                     /gene="proB"
                     /locus_tag="VFV99_11845"
     CDS             8046..>9126
                     /gene="proB"
                     /locus_tag="VFV99_11845"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5060048.1"
                     /translation="MSERSELVNAKRVVIKIGSRLIQESPASRPATVADQVVELRRRG
                     IEVVIVSSGAIALGLPRLQMKQRPTEMPELQAAAAVGQSRLMQHWEHAFGAHDIEIGQ
                     VLLTHDDLGDRRRFLSARLTLRALLDHGVVPIINENDTVATEEIKFGDNDQLAALVSN
                     LVSADVLVILTDVEGVRDAQGVRMPIVRDIDREAAPVAGSTGSSVGSGGMASKVGSAR
                     IVTRTGVPAVVAPGREADVLIRILAGNDVGTLFVPNGRELSSRKHWIAYGSKPVGKLT
                     VDDGAARALRDGGKSLLPAGIVAVEGDFELGDTVSIVTRDGRELGRGLAAYPGDDLRK
                     IRGLHSAGIEATLGYKSIDEAIHRDD"
BASE COUNT         1493 a         3120 c         2976 g         1537 t
ORIGIN      
        1 ccatctgcag tcgttcgcgc agcacctcgg ttacggcgtc gcgaccgccg cgaccacgcg
       61 cttcctcgag cagcgcatgt gatcacggct ccgcgagcag ctcgcgcgcg agtgttggtg
      121 cacgcagcgc ttcggggccg atcaagcgat agcgcagccg ccgcttgcgg tcgtagacgt
      181 tcacgtgcgg cagcgtaccg atgtcgtacg ccttcgcgac ggccgtgaac ccgtcgccga
      241 cgtcgacctt gcgcacgatc acctgcggct gcttcgcgat ctgcaccgca atcatgccgg
      301 tgacgatcgt gcatgcctcg catgacgtgc tccagaagtc gacgatcgtc acgtagccgt
      361 cgacgagtga atcctcgacg tgaacgagcg caccatcggt gttgagctgg accgtcttgt
      421 cgaccggcgt tggcggcggc gtgctcgcac cacaggccgc gagggcgagg agccagcgct
      481 tcactgtgac tcggcgagct gcttcacgag ccgcgcgacg tccgtgcgcg agagccccgc
      541 gtgatagacc tgctcgatca tcacgcgatc gccagacacg agctcgctcg cgccgagccg
      601 cagcacgcca tccgtatcga tcgtgacctc gacgagcacc agcacgtctc cgcccggcgc
      661 ctcgggaaga tcgaccacgg cgtagcggcc gaggtggcga ttcgccgacg gatctgccga
      721 ctcaccctcc cagagatcga actcgacgcg tacttgatcg tcgtggcgcg tcggcagcag
      781 cttgtgctcg cgcgtcggga tcacggagct ctgcgcgatc accggctcgc agtcctggcc
      841 ctgtcgcgac atcatgagcc cgcgcgctgc cacatcgatc aacaagacgc cttcgatcgt
      901 gccgtcgagc cgcgcaacct cgagcgctgc accgaccgcg acgacctctt cggggttcgc
      961 gatcacgctc ggctcgcggc cggtcattcg cgcgagctcc ttgcgcacga ccggcatgcg
     1021 cgtgctgcca ccgacgagca gcacttcttc gatcgcgtcg cgctggcggc tcgagcgaag
     1081 caacgactcc ttcaccggcg gctcgagccg cttgacgagc ggcgacgtcc acagctcgag
     1141 ctcgtcgcga cgcagcatgc gcacgtactc gagatgcttg ccgcttggca gctgtgcgag
     1201 atccggaatc tgcagcttcg cggtcgcgtg gtccgacagc tcgtgcttcg caagctgcgc
     1261 ggcgaggcgc agcttctcga tcgcgctcgc gtccgtcgac acatcgatac cgcggctctg
     1321 gcgcacgtcg cgcacgaggt tctcgacgag cacgcgatcg acatcgtcgc caccgaggaa
     1381 tggatcgccg gtcgtcgcga gcacctcgaa cacgcccgca tcgacatcga cgagcgcgac
     1441 gtcgaacgtg ccaccaccga gatcgcacac gagatagcgg cgatccgcgc cgcggtgcgc
     1501 cccgtgaccg agcgcagcgg ccgttggctc gctgagtagc cgcatcactt gcaacccggc
     1561 gatctgcgcg gcgtccttcg tcgcctggcg ttgcgcggcg tcgtaccagg cgggcaccgt
     1621 gatcaccgcg cgcgtcaccg gctcgcggaa gaaccgttcg gccgtgcgct tgagctcgcg
     1681 caggatcaac gccgagactt cctcgggcga caccgcgcga ccggcggcga gctggatcca
     1741 ggtatcgcca ttcggcgcct cggtgagctc gtacggcaac acgcgtgcga gcttgcgcac
     1801 ctcgggatga tcgaagcgcc gaccgagcaa gcgcttcgcg ccgaagatcg tgagatcggg
     1861 gctgtgctcg agcccttcgc gagcggcttc gccgacgatc ggcccctgcg gcgagaacca
     1921 cacgagtgac ggcagcatcg tcgagccctc ttcggtcgtc aggatgcgtg gcgcaccgtc
     1981 gcgatcgatc accgcgacca ccgagttcgt ggtgccgaga tcgatgccaa ccgccctgcc
     2041 cttcaccgga acaacctccg catcgcatcc gcttcggcgg tgccgtggcg gcgaatgtgc
     2101 tcgagcatct tcgatgcttc ggagcactgc tcgtgatgcg cgagcgcggt ctcgagctgc
     2161 gcggtggcaa gcatcagctc gccgtccagc acggctgcga gcgccgtcgc gacgtaatag
     2221 agcgagcgca gcggcttgct gcgcggatag acgacaagcg cagccgcgag cacctcacgc
     2281 gcggcgttcg catcacccag cgcgagcatc gcgcgtgcgc gtccttcgta ggcctcgctg
     2341 ccctggcccg atgcagcgct cggcggcggt ggcggcggca cgggttgcgg cgtcggcggc
     2401 cgctcgaacc ggcgcagcga acccttcggt ggcgtcgcgt tcgccggatc gctcttgatg
     2461 tcctcgaacg cgacgagcca gcccgcaggt gatgcgaccg tcgggcccgc tgtcgccgca
     2521 cgcccttcgg caacgagcga cgtgcgcatc cgatcgtagg cgcggtttgc gagaatcgtc
     2581 agctcctcgg cgaggtgcgt gatcgcgggc gcgttgcggc gtgcaaccag atcgggatgg
     2641 ttgcggcgaa tcagatcctt ccagctcgcg cgcacgagct cggggcccgc attcggcggc
     2701 acgccgagca cgtcgtgcac cgccgcctga cgcagacggc ggagctccgc ggtcaggtgc
     2761 tggaacagct cgcgctcgtc ggccggcaag tcgtcgccgc caatctgcga ctgcgatcga
     2821 cgcacgggtg gcgcaggcgt caccaggtgc gccgccgcga tattcggatc ggtctccgga
     2881 tcctcggcgt cgcgctgtag tcgcttgagg cgtgctttga cctcgtcggt gaagccggtc
     2941 agctcgatct cgatccacgt gcgcgacttg tcgaccttgc cgtcgacctc gactggcgcg
     3001 gacttctgga cgacgccctc gatcgcgacg accacctcgt tcggcagctc gagaccgatc
     3061 gtcacctgca tgccggtctt cgccgagaac ggcaccttca tcgaaagcag cttgcccttg
     3121 cgcagtttgc gggtcgtgaa gatgtcgacc tgctcccagc tcgagcaccg cacttgcagc
     3181 gttcgcgacg ccactacttc cccttcttcg ggggcttgta gtctggatcg ggctcgagct
     3241 cgaccgtccg cgacaacatc ggcttcttct tcgcgacatc gatcggcgtg ctcggatctc
     3301 cgccgtcgcg ccactcgtcg gccttgaaca tcacgtgctg cgtcttgaag ccgggtttga
     3361 ccaccgctgc ttcgtagtcg cgtcccgcga tcagctcgtt gaactgcgcg tggttcgcgc
     3421 cgatgaacaa ccacacctcg gcgtctgccg gagtcgactc gatgtgcacg atgccagcgc
     3481 cgaccactcc ggtggaaccg agcacaggcg tcgtcggttg cagcggaagc actggctcgg
     3541 ccttcgcctt cgacggcttc atcgtcacgg tgagcttcgc cttgagcgct tccttcgcgc
     3601 cgctccagct cgtgccgttg acttgtgcct cggtgacctc gttgccgctg tgcaggagca
     3661 ccatgtcgtg cgcctgatcg gcgtgcagct tgatcgtcgt gtcgagcggc gtcttgccca
     3721 acttgagcca gatgcccgcg cccgacgacg tgatgtcgag cgcgcccgag tcggcttgcg
     3781 cctcgcgcag ctgcttttcg atgtcgtcct tctctttgag ctgcttctcg cgctcttcct
     3841 tggcctgcgc ctcgcgcgct ttgtcttcct ggaatttcgt gtagacgacg tagccagcac
     3901 cgccgagcac gccgagcacg agaaggcccg cgacggcgct gcgcagcggg ctcttcagcc
     3961 ttggcgtcgg catgtcgtcg tcgcggatcg gatcgacgat cggctgtgcg cgcggcgcgg
     4021 gtttgcgctg ctcgggtttg cgctgctcgc tctcgacgcg cttcgccgcc tgctcgattt
     4081 tctgactcga cgcgcgccgc gcgcccgacg catccagatc gttgatcgca tcgagcgccg
     4141 cggcctcgtc ggcagacgtg gtaccgcgcg acggtggcgg cagcggcgtc gcgtcatctt
     4201 cctcgtcgtc cggatcatcg agcgacggca cgcgcggcac cggcatgcgc cgctcgttgc
     4261 gaatgacctc ggagatcgga tcgcggcgct tcgcggcacc ggccggatgg tcgtcgacct
     4321 gtgtgaggcc ggtgtcatca ccgccgccga gatctgcgag caacgcgtcg agccggttct
     4381 gggtgctgtt cgcacgctgg tgtttggcgc cgagcgcttc gacgtccttg cgcggcggcg
     4441 tcgtgacctt cacctcggac ttaccgagcg acgcgagcag atcctcggtc gcgtcgtcga
     4501 tgttctcgtc ggtcgccgac agcccggctt ccttgatgag ctcgccacgg tccgcgagct
     4561 cccagcgatc gtcttccatc gcctcgatca agttctcgag catgtcgctc gcggtcttgt
     4621 agcggcgcgt cggatcggta tcgagcgcgc gctgtacgag ccgctcgacc gcgggcgtca
     4681 cgcgcagcga gccgggcggc acttcgcccg acagcatcgt gaacatcatc gcacccacgg
     4741 caaacacgtc gacgcccggc gtcggatcct cgccaacgac gagctcgggc gcgatgtagc
     4801 cggtgagccc gcgccatagc gacgagtcag caccttgtgc gactgcggtg gtgagcgcga
     4861 gcccggcgat gaagtcggcg agcagcacgt tgccgtcctc gccgatcagc acgctgcgcg
     4921 gatgcacggc gccgtgaatc acgcccgcgc ggtgagccgc ggcgagcgct tcgatgaccg
     4981 acttgccgat ctgtcctgcg accggcgtcg acagcttgcc gccctgcttg cctcgcgcct
     5041 tcgcggacgt gatcagatcc tgcaccgtga cgtagcggcc aacgccgcgg gtaacgatca
     5101 cgacatcagg gccgcccgac tccacggtca tggtcggcac gatgttcggg tgctcgagct
     5161 tggtgagctt cgcgatgttc ttcgcgtcgg tcagcgaaat ccggaaggcg tcttcttcca
     5221 gcatccgcgg atcgacgacc aagccgcgca agttgcgctg cccgccgaac gacgcgcgat
     5281 ggatcgcacc gtacatcgtg accgcgagac gctcgctgag cacgaccccg gcgatgatct
     5341 gctcggccgc ttggcctgcc atcgtctact gactatagca gccggaagta cacgttcaga
     5401 aacggctcgg cgaacaaata gaacaccgcc gcgatggcca gaaagggccc gaatggcagc
     5461 tccgactgca tgatttgctg tttctttcgc accagcacgg cgatgccgat gatcgagccg
     5521 accaccgagc cgccgaatag cgacacgacc acacctcgcc agccgaacag tgccccgatc
     5581 agtgcgagca tcgtggcgtc gccgaggccg agcccctcgc gcttgcggat gcgcaaatac
     5641 agctcgccga tcagccacgg taggccgtag ccgatggcga tgccgacgag gccctcccac
     5701 cacggccgaa acgcaagact cgcggcatag aacgcgacga tcgccgggat cgtgaccttg
     5761 ttgaggatca gcttgtggtc gatgtcgatg aacgtgatca cgaccatgac ccacacgaac
     5821 gcggcgtaga ccacgaagtg caaaagccgc gtgtcgaatg gctggaataa gccgcccgtc
     5881 ccgagcgtga accaccacgc gcaaccgaac agcgcgccgg tgaccgcctc gaccagcacg
     5941 tagcgcggcg agaactcggc cttgcagttg cggcagcgcc cgcgcagcca caggtagctg
     6001 aagagcggca cgttgtcgta ccagcggatc ggtgccttgc acgcaaagca atgcgagtcg
     6061 ggcttcacga ccgatcggcc cttcgggaat tcctcggtcg gcggccagcg gtagatgcag
     6121 acgttcgcga agctgcccca tacgaggccg agcacgaccg cgacgaccag gcctccgatg
     6181 gaggtcgcaa gcggctggag ctccgacacc tggaacaccg tagctcggtt gaccgtagcc
     6241 gtcgagaaag ctaagaccgt tgggaatgcg ccgcctcgcg ttgtttgcct gcttggcact
     6301 agtagggtgc ggtcgctccg aaggcgtgcc ggacgagcaa ctcggtggtc tcgtcattgc
     6361 cccgaagaca aaagccgcgc cgatcgatgt cacgcgggca gcgaaagatc cgcgcgagct
     6421 tggccgcgca ctgtcgctac cgtacggcga cgtgatcacc gcgctcgggc cccacacgta
     6481 cgcgatcacg acttccacgg tcgtcgaaga aggcggcaag aaggtcaacg agctctcgga
     6541 tcaaaccaag atcgacctcg gtgacaacgc ggcgttctcc gccgtctaca cgaactcggc
     6601 cgactacggc cgcgaagcga tctcgattgg cggtcagctg tatctgcgtc cgcgctatca
     6661 gcgctggcac ggtcgcgcgc ccgagatgcc cgacgagccg gcgcagatcc gcgactcgtt
     6721 cttcgtgccg ctcgccgcga cgtgggagct gttcgcgccg ggcgccgagc tgagcgatct
     6781 cggaaccgcg caggtggcgg gtcgcagcgg caagaagatt gccgtcaagc tgtcgccgaa
     6841 tggcgcgaag ccagccgccg aaacggtgag tcagcgcaag tggcgcgaga agcgctcgat
     6901 cgaaagcctg agcggcgaga tcgtcatcga cgccgacaaa ggcgttccgc tgagcgtgaa
     6961 gctcgcaggc acgatcggat tctcgcgcga tggccgccac ttcacgatga aggtctcact
     7021 cgaggcaagt gcgacggcgt tcggcaaagt cgcgatcgcg gcaccgcccg ccgaccaggt
     7081 cgtcgccacg cccgagcgct tgcgggaagt cgacgaccgc gacttcctct tgcagggtat
     7141 tgccccgcct ctgcgccgaa atcccgatgg aaccgccgtc acgccacagc cgaaaatggc
     7201 tgggcccggc tcagtcactc cacaaccgaa ggaagctggg tcaaactcgg gtaaatgacc
     7261 gcgctcgtcc agcgtcgcga ttacctcgtc ggccagcgcc gcacgatcat cgcgcgctcg
     7321 ttgatgggca gccttgccgg tgcgttaccg attccgttcc tcgatgactg ggcggtcggt
     7381 cgattactcg gcggcgggta ccgcaagatc gcggccgctc accaggtcga catgaccgat
     7441 gaagcggtcg acaacatggt gcacggcccg acgaagcctc ctccgatcgc gcagatggcg
     7501 atcggcggcg tgctctcgcg tgtcgcgagc aaagctgcgc gccgcatgat gctcgcgatc
     7561 gcgaccgtca accgcgcgcg gtcggcggca cgcacgttca cggcgatgac gctgttcgat
     7621 cactactgcg cgaagctgca caccggcttt ggcctcgacg gtgcgaccgc gctcgcgttg
     7681 cgcgaagaaa tcattcgcac gatcgatcag acgcccggtg cgctcgcgtt ccatccgttc
     7741 cgccgcggtg ccttgtcggc cgcgcgcgcg acgctgaagg cgccgctcga gctcgcggac
     7801 ctcgcctccg gcggccggct gcgcaagctg ctctcgaaca agagcgagat cacgacgccg
     7861 cagaccgtcg acgaggtcga gcaagcgatg gaatccgtgc tcgccgacaa gacgacgttt
     7921 ttgtcgcgca cggtcgccgc cgtcgaggtt cagctgtcgg cggaggtcaa tccgtacctc
     7981 gacgacgtgc tcgagtctct cgatcgccga tggcgtgcgc gggtggcggc gaaagccgat
     8041 ctgaaatgag cgagcggtca gagctagtca acgccaagcg cgtcgtcatc aagatcggca
     8101 gccgcttgat tcaggaatcg ccggcgagtc ggcccgcgac ggtcgcggat caagtcgtcg
     8161 agctacgccg gcgcggcatc gaggtcgtca tcgtatcgtc gggcgcgatc gcgctcggtt
     8221 tgccgcgcct gcagatgaag cagcggccga ccgagatgcc cgagctgcaa gcagcagccg
     8281 cggttggcca gtcgcgactc atgcagcatt gggagcacgc gttcggtgcg cacgacatcg
     8341 agatcggcca ggtgctgctc acgcacgacg acctcggcga tcggcgacgc tttctgtccg
     8401 cgcgcctcac gctgcgcgca ctactcgatc acggcgtcgt gccgatcatc aacgagaacg
     8461 acacggtcgc gaccgaggag atcaagttcg gcgacaacga tcagctcgcc gcgctcgtct
     8521 cgaacctcgt atccgcggac gtgctcgtga tcctcaccga tgtcgaaggc gtgcgcgacg
     8581 cgcagggcgt acgcatgccg atcgtgcgcg acatcgatcg cgaagcggct ccggtcgccg
     8641 gctcgaccgg cagcagcgtc ggttcgggcg gcatggcgag caaggtcggc tccgcccgca
     8701 tcgtcacccg gacgggtgtg ccggcagtcg tcgcgccggg ccgcgaggcc gatgtcctga
     8761 tccgcatcct cgccggcaac gacgtcggaa cgctattcgt gccgaatggc cgcgagctgt
     8821 cgtcgagaaa acactggatt gcgtacggca gcaaacctgt gggcaagctg accgtcgacg
     8881 atggcgccgc acgcgcgcta cgagatggtg gcaagagcct gcttcctgcc ggaatcgttg
     8941 cggtcgaggg cgacttcgag ctcggggaca cggtctcgat cgtcacgcgc gatggccgcg
     9001 agctgggccg tggtctcgcg gcctacccgg gtgatgatct gcgcaagatc cggggcttgc
     9061 attcggccgg tatcgaggcc acgctgggat acaagtccat cgacgaagct attcaccggg
     9121 atgatc
//
LOCUS       DASUFX010000302         7916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_324586,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000302 DASUFX010000000
VERSION     DASUFX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324586"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFV99_11850"
     CDS             <1..1116
                     /locus_tag="VFV99_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5060049.1"
                     /translation="QTLARGRADDLELLDWLRNVIWPYEAALDGEAASAAGELACAEL
                     LLGGTTAILDMATVHHTDAIFSAAERAGIRATIGKAMMDAPDPQIPPGLRETTKHSLD
                     ESATLIKRWHGAANDRLRYAYAPRFVLSCTDELLREVGTQSRARGVGIHTHASENKGE
                     VALVRQRFGKDNIVVLDELGLLGPHTCIAHGIHLSDQERALLASRGAHICTCPSSNLK
                     LASGVCALPELIAAGVSCAIGADGAPCNNNLDGFMELRLVALLHKPRVGPRALPAPEV
                     VRLATIGGAAALGQADRIGSLELGKRGDVIAVDVTPLHTIPTGSPWSQIAYAAKSCDV
                     RHVAVDGNVVVREKTLLTLDLGKVRARARDATVKLFP"
     gene            1233..1562
                     /locus_tag="VFV99_11855"
     CDS             1233..1562
                     /locus_tag="VFV99_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5060050.1"
                     /translation="MSNRLYVGNLPFHATEDLISQRFATCGEVREVALMLDRMTGQSR
                     GFCFVEMATAEGAEKAIQNLNGQDFEGRSLRVDVAQERPRGGGGGGGGGGGFRGNDRG
                     GDRRRGR"
     gene            1552..1980
                     /locus_tag="VFV99_11860"
     CDS             1552..1980
                     /locus_tag="VFV99_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060051.1"
                     /translation="MAAEGASSNELAASVTSEGTGGEPSHGQPANSNARSARGTGEVA
                     TEAARPEPARTVVSVPHELTELATSIGQAWSQEYVRQLQAQERDIVGAWPGTIREARR
                     QVLAKVKAKLEPEQLEQLAKITNLAARRGWETVSEPDLES"
     gene            1983..2261
                     /locus_tag="VFV99_11865"
     CDS             1983..2261
                     /locus_tag="VFV99_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060052.1"
                     /translation="MAIPLRLAGRPEKIFVSVDESSGKSTISFQPADVGPPVQHEDDK
                     TGSTAMQEARSISEKYPGCTVHGPHFHAARPPGARWKRRRSEPKGDEE"
     gene            2309..3199
                     /locus_tag="VFV99_11870"
     CDS             2309..3199
                     /locus_tag="VFV99_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5060053.1"
                     /translation="MLLTREEMTFDFGGAKLDPVRDRELLEWMFNQFLYGEVTGIQVG
                     HWLYDAPDLEAAKFLARQSLEEMQHVDNFLRIMTMLNCQPKPAHAAVRFLSTGMMGGS
                     WAEHVALEMAQGEGFVLQAFYAVIDTLDHKPTVDILKRAVKQEERHVEFGEDQTKKAI
                     EGKGWLRRRLLGLSLVSMWGVRKLAGYMEKKLPADHVVLKHMPQFLAHANRCAEIRLR
                     RIGVLDRPLEQLSGAKRALLVAEAYGGKFLGGIGSLLATPLKLLPWFRKKRVTDTYLL
                     DPHVTGYQLPAPDSRAELEA"
     gene            3215..4714
                     /locus_tag="VFV99_11875"
     CDS             3215..4714
                     /locus_tag="VFV99_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060054.1"
                     /translation="MVRAIVDELLRRPWLALVLALLVQIVVFVFGQSDLPVADPLWYA
                     NIAHKIAVDPSTVFDHAEIHPFVMRVGLTMPVAVLYKLFGVSTLTTNLYALWAALGVI
                     IIVYAAAPSPRAKLLGMVLATFCVALLRNANVLNVDLPAATWMGAAVLCLSRRDRPRG
                     TWWIVGAAVATIAAFLVKETAVWLAVVWLYTLAVDVRSDARAALRRFAPGLVVGAVLT
                     AAYLWVCARVWGSPFARFSGIEELTYEHTWSLQGKPASAWVNRLTWQAPWFLLAQFQL
                     LLPIALASPWLVRGRLRIWVVAAAAFVLLFWFGSSSLSAYTPLPLVPRMVMPILPVLL
                     VVATLTADAAIDRVRDASWRIALAIVLALALVIPTGRAMLKLVLRQHPETDAFAIVRH
                     TAATGNSTIVVCAEERCGAAGNFHFGFEIPANVSFNSVTEYASQYRNDAHVFVLVNLH
                     RIGPQSEHVKLIEALHLPSLTGHRYVKLYDAGDGAALRLALQSTLHWDP"
     gene            complement(4718..5803)
                     /locus_tag="VFV99_11880"
     CDS             complement(4718..5803)
                     /locus_tag="VFV99_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5060055.1"
                     /translation="MKRAAAIAILVLAGCPTRGGKTTVSNDQTVVTDSDLERRQRLIG
                     ELQDEVLASYERDELPEVETTMIPPDVGPARIGVGPGDVLFGDDVRRLAWSRWPLQLE
                     PGTQAIARSKALEIHLSHDKQVSAAWMSDELSWRITMCNRTAVIPMRITALYAHDGDR
                     WVQVFEHLSFGRTPQPTADGKLRGAAIREAVIDRGLSDDLSRALNGLLSAQATRVKLS
                     VSPTKTDDPTQPAPTFLLGPDPDAEWAGGQIESIRLVDGKVTLDERRVGVVTAGRPDK
                     ATIAYWIGNLVADLRHHPGTPASKVLLRGTFIFEKRDDKWVIVQGHISQPIDDIDLAT
                     YVYGTALIREKPLQITCEDGSRSARQQ"
     gene            complement(5800..6495)
                     /locus_tag="VFV99_11885"
     CDS             complement(5800..6495)
                     /locus_tag="VFV99_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5060056.1"
                     /translation="MKIGAVILAAGAGTRLGGVAKALLTGSDGRTFIAKVVATAREAG
                     TDDIVVVVGPPFADAVATQARELGVRVVVNPQPERGMASSVAAGFGALDRADVGVQDV
                     GGAMRDIGGTARDIANLAGREDPRCSVRPDDMRVDAAWLWPVDHPHVDASTLRSLISA
                     LIDHDAARPVYRGRGGHPPLIARSLFERLATCANADGGARAVLAAADTIDVPVDDRSC
                     VRDVDTPADLEVV"
     gene            complement(6568..7569)
                     /gene="xdhC"
                     /locus_tag="VFV99_11890"
     CDS             complement(6568..7569)
                     /gene="xdhC"
                     /locus_tag="VFV99_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEX5060057.1"
                     /translation="MAVDVFAAALRALDGGRGVAIAAVTHASGSTPRHLGARMAVAAD
                     GEQWGTVGGGRIEALVVSAAKEVAAGAAPRVVRQHLVRDLAMCCGGSMEVAITPAAPS
                     REAIEKLAGARKALVLVTPLDGGPLLVREPGDQDPRAHQPLVADGSLVERLGTAERAI
                     LFGVGHVARTLGPQLAKLGFAVIVCDDGETGAIDEPPAWADRVVESFDAAAVERELGG
                     FGADDYVLIVTRDHAIDLKLLEQLIGRDELHYLGMIGSRGKIGRFRKRLEAKGIIDDS
                     DLGKQRWARLRAPIGLDIGAETPEEIAIAVAAELVACRRRGAPSVGDWRPSKGWREE"
     gene            complement(7621..>7916)
                     /locus_tag="VFV99_11895"
     CDS             complement(7621..>7916)
                     /locus_tag="VFV99_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060058.1"
                     /translation="DASFDELCDAARAALRDLGPVHVGTPSADFNTARLDGWFPDAPI
                     WFVSFDHPSIATVIESDGADLAVGIMARALRQQDHDEQTVVVVRRFDGTIRPH"
BASE COUNT         1270 a         2741 c         2570 g         1335 t
ORIGIN      
        1 cagacgctcg cgcgcggccg cgccgatgat ctcgagctac tcgattggtt gcgcaacgtg
       61 atctggccgt acgaggccgc cctcgacggc gaggccgcga gtgccgcggg cgagctcgcg
      121 tgcgcggagc ttcttctcgg cggcacgacc gcgatcctcg acatggcgac ggtgcatcac
      181 accgacgcga tcttctcggc cgccgagcgc gccggcattc gcgcgacgat cggcaaggcg
      241 atgatggacg cgccggatcc gcagatccca ccgggcctgc gcgagacgac gaaacactcc
      301 ctcgacgaat cggcgacgct catcaagcgc tggcacggag ccgcgaacga tcgcctgcgc
      361 tacgcgtacg cgccgcggtt cgtgctgtca tgtaccgacg agctgcttcg cgaggtcggc
      421 acgcaatcgc gcgcccgagg cgtcggcatc cacacgcatg cgagcgagaa caagggtgag
      481 gtcgcgctcg tccgccagcg gttcggcaag gacaacatcg tcgtcctcga cgagctcggc
      541 ctgctcggtc cgcacacgtg catcgcgcac ggcatccatc tctccgatca ggaacgcgca
      601 ctccttgcat cgcgtggcgc gcacatctgc acgtgcccgt cgtcgaacct caagctcgca
      661 tcaggcgtgt gtgcgctacc cgagctgatc gccgcgggcg tctcgtgcgc gatcggcgcc
      721 gatggtgcgc cgtgcaacaa caacctcgac ggcttcatgg agctgcgcct cgtcgcactg
      781 ctgcacaagc cgcgggtcgg cccgcgcgca ctacctgcgc ccgaggtcgt gcgcctcgcg
      841 acgatcggtg gcgctgctgc actcggccag gccgatcgca tcggctcgct cgagctcggc
      901 aagcgtggcg acgtcatcgc cgtcgacgtc acgccactcc atacaattcc gacgggaagt
      961 ccgtggtcgc aaatcgcata tgcagcgaag tcgtgcgatg ttcgccacgt tgccgtcgac
     1021 ggaaacgtcg tcgtgcgcga aaaaacgcta ctcacgctcg atttgggaaa agtacgggca
     1081 cgtgcgcgag atgccacggt gaagttgttc ccctaggggg cgcgtgctac accgtcggca
     1141 ctcgtctcgt tgagggcgat tacgacttcg ccttccgagc agctggttgg tgttcgtctt
     1201 tttttcgaag ctgccagaga gaacagtccg aaatgtcgaa tcgtttgtac gtcggtaacc
     1261 ttccgtttca cgccaccgaa gacctcatct cgcagcgctt tgcgacctgc ggcgaggttc
     1321 gcgaagttgc attgatgctc gaccggatga ccggtcaatc gcgtggtttc tgcttcgtcg
     1381 agatggcgac ggctgagggc gccgaaaagg cgatccagaa cctcaatggt caggatttcg
     1441 aaggtcggtc gctccgcgtc gatgtcgcgc aagagcgtcc gcgtggcggt ggcggtggtg
     1501 gtggcggcgg cggcggtttc cgcggcaacg accgcggtgg cgatcgtcgt cgtggccgct
     1561 gagggcgcaa gcagcaacga gcttgccgcg tcggtgacct ccgaaggcac cggcggcgag
     1621 ccgtcgcacg ggcaaccggc gaactccaac gcgcgcagtg cgcgtgggac cggcgaagtt
     1681 gcgaccgaag ccgctcgtcc ggagcctgct cgcacggtcg tgtcggtgcc gcacgagctc
     1741 accgagctcg cgacgtcgat cggccaggcg tggtcgcaag agtacgttcg tcagctgcaa
     1801 gcgcaggagc gcgacatcgt tggtgcgtgg cccggaacga tccgggaggc gcgccgtcag
     1861 gtgctcgcga aggtcaaggc gaagctcgag cccgaacagc tcgagcagct cgccaagatc
     1921 acgaacctcg ccgcacgtcg cggctgggaa accgtttccg aaccggacct ggagtcgtga
     1981 tcatggcaat ccccctacgt ttggctggcc gtcccgagaa gatcttcgta tcggtcgatg
     2041 aatcgagcgg taagtcgacc atctcgttcc aaccggccga cgtcggcccg cccgtgcagc
     2101 acgaggacga caagacgggc agcactgcaa tgcaggaagc ccggtcgatt tccgagaagt
     2161 atccgggttg cacggtccat ggcccgcact tccacgcagc gcgtcctccc ggcgcgcggt
     2221 ggaagcggcg ccgctccgag ccgaagggcg acgaggaata gtcggatcgg ggcacgagcc
     2281 ccggtgctat cctgcgcgca ggtatccgat gctgctgacg cgcgaggaga tgacgttcga
     2341 ttttggtggg gccaagctcg atccggttcg cgatcgcgag ctgctcgagt ggatgttcaa
     2401 tcaatttctc tacggcgagg tcaccggcat ccaggtcggt cactggctgt acgacgcgcc
     2461 agatctcgag gctgcgaagt tcctcgcgcg ccagtcgctc gaggagatgc agcacgtcga
     2521 caactttttg cgcatcatga cgatgctgaa ctgccagccc aagccggcgc atgccgcggt
     2581 gcggttcttg tccaccggca tgatgggcgg ctcgtgggcc gagcacgtcg cgctcgaaat
     2641 ggcgcaaggc gaaggcttcg tgctgcaggc gttctacgcc gtcatcgaca cgctcgatca
     2701 caagccgacg gttgacatcc tcaagcgcgc ggtgaagcag gaagagcgtc acgtcgagtt
     2761 cggcgaggac cagacgaaga aggcgatcga gggcaagggc tggctgcgcc gccgcctgct
     2821 cggcctctcg ctcgtgtcga tgtggggcgt gcgcaagctc gccggttaca tggagaagaa
     2881 gctgccggcc gaccacgtcg tgctcaagca catgccgcag ttcctcgcgc acgcgaaccg
     2941 ctgcgccgag atccggctgc gcaggatcgg cgtgctcgac cgtccgctcg agcagctctc
     3001 gggtgcgaag cgcgcgctgc tcgtcgccga ggcctacggc ggcaaattcc tcggcggcat
     3061 cggctcgctg ctcgcgacgc cgctcaagct cctgccctgg ttccgcaaga aacgcgtgac
     3121 cgacacgtat ctgctcgatc cgcacgtcac cggctatcaa ttgccggctc cagattcgcg
     3181 cgccgagctc gaagcgtagt tataacgacg ctcgatggtt cgcgcgatcg tcgacgagct
     3241 gctgcgccgg ccctggctcg cgctcgtgct cgcgctgctc gtccagatcg tcgtgttcgt
     3301 cttcggccag agcgacctgc cggtggcgga tccgctctgg tacgcgaaca tcgcccacaa
     3361 gatcgcggtc gatccgagca ccgtgttcga tcacgcagag atccatccgt tcgtgatgcg
     3421 cgtcgggctg acgatgccgg tcgccgtgct ctacaagctg ttcggcgtct cgacactcac
     3481 gacgaatctc tacgcactgt gggctgcgct cggcgtgatc atcatcgtct acgccgccgc
     3541 accttcgcca cgcgcgaagc tactcggaat ggtcctcgcc acgttctgcg tcgcgctcct
     3601 gcgcaacgcg aacgtgctca acgtcgatct tcccgccgca acgtggatgg gggccgccgt
     3661 actgtgcctg tcgcgccgcg atcgtccgcg cggcacgtgg tggatcgtcg gcgccgctgt
     3721 tgcgaccatc gccgcgttcc tcgtcaagga aaccgcagtg tggctcgcgg tcgtctggct
     3781 ctatacgctc gccgtcgacg tgcgcagcga tgcgcgcgcg gcgctccggc ggttcgcgcc
     3841 gggcctcgtc gttggcgccg tgctcacggc cgcgtatctg tgggtatgcg cgcgcgtgtg
     3901 gggcagtcca ttcgcgcgct tctccggcat cgaggagctg acgtacgagc acacgtggtc
     3961 gctacagggc aaaccggcga gcgcatgggt caatcggctg acctggcagg caccatggtt
     4021 cctgctcgcg cagttccagc tacttcttcc gatcgcactc gcgtcgccgt ggctcgtgcg
     4081 cggccggctg cggatctggg tggtcgcggc agcggcgttc gtcctgctgt tctggttcgg
     4141 ttcgagcagc ctgtccgcct acacgccgct tccacttgtt ccgcgcatgg tgatgccgat
     4201 attgccggtc ttgctcgtcg tcgcgaccct gaccgccgac gccgcgatcg accgcgtgcg
     4261 cgacgcgagc tggcgcatcg cccttgcgat cgtgctcgcg ctcgcgctcg tcatcccgac
     4321 cggtcgcgcg atgctcaagc tcgtcctgcg ccagcatcct gagaccgacg cgtttgcgat
     4381 cgttcgccac accgcagcga ccggcaacag cacgatcgtc gtctgcgcgg aggagcgatg
     4441 tggggcggca gggaacttcc acttcggctt tgagatcccc gcgaacgtct cgttcaattc
     4501 ggtgaccgag tacgcgagtc agtatcgcaa cgacgcgcac gtctttgtgc tcgtcaacct
     4561 tcatcgcatc ggaccgcaga gcgagcacgt gaagttgatc gaggcgctcc acttgccctc
     4621 gctcaccggt caccgctacg tgaagctcta cgatgccggc gacggcgcgg cgctccggct
     4681 ggcgctgcaa tccacactgc actgggatcc gtagcgccta ctgctgccgc gcgctgcggc
     4741 tgccgtcttc gcacgtgatc tgcagcggct tttcgcgaat cagcgcggtc ccgtagacat
     4801 aggtcgcgag atcgatgtca tcgatcggct gcgagatgtg gccctgcacg atcacccatt
     4861 tgtcgtcgcg cttctcgaag atgaacgtgc cgcgaagcaa caccttgctc gcgggcgtgc
     4921 ccgggtgatg acgcaggtcg gcgacgaggt tcccgatcca gtacgcgatc gtcgccttgt
     4981 ctggccgccc ggccgtgacc acgccgacgc gccgttcgtc gagcgtcacc ttgccatcga
     5041 cgagccggat cgattcgatc tggccgcctg cccactccgc gtcggggtcg gggccgagca
     5101 agaacgtcgg tgccggctgc gtcgggtcat cggtcttcgt cggcgacacc gacaacttca
     5161 cgcgcgtcgc ctgcgcggac aacaggccgt tgagcgcgcg agacagatca tcggacaagc
     5221 cgcgatcgat caccgcctcg cgaatcgccg cgccgcgcag cttgccgtcg gcggtcggct
     5281 gcggcgtgcg gccgaacgac aggtgctcga acacctggac ccagcgatcg ccgtcgtgtg
     5341 catacagcgc cgtgatgcgc atcggaatca ccgccgtgcg attgcacatc gtgatccgcc
     5401 acgagagctc gtcggacatc cacgccgcgc tgacctgctt gtcatgcgag agatggatct
     5461 ccagcgcctt cgagcgcgcg atggcctgcg tccctggctc gagctgtaac ggccagcggc
     5521 tccacgcgag ccggcgcacg tcatcgccaa acagcacgtc gccggggcca acgccgatcc
     5581 gcgcgggtcc gacgtcgggt ggaatcatcg tggtctcgac ttcgggcagc tcgtcgcgct
     5641 cgtacgacgc gagcacttcg tcctggagct cgccgatcag gcgctggcgg cgctcgagat
     5701 ccgagtcggt cacgaccgtt tgatcgttcg atacggtcgt cttgccgccg cgcgtgggac
     5761 aaccggcgag tacgaggatc gcgatcgcgg cggcgcgctt cacacgacct ccagatcggc
     5821 gggcgtgtcg acatcgcgca cgcagctccg atcgtcgacg ggaacatcga tcgtatcggc
     5881 cgcggccagc accgcacgcg caccgccgtc ggcgtttgca cacgtcgcga gtcgctcgaa
     5941 caacgagcgc gcgatgagcg gcggatggcc gccgcgtccg cggtacacgg ggcgcgccgc
     6001 atcgtggtcg atcaatgccg aaatcaaact ccgcaatgtc gacgcgtcga cgtgcggatg
     6061 atcgacgggc cagagccatg ccgcatcgac gcgcatatcg tctggacgta ccgagcaccg
     6121 cggatcttca cggccggcca ggttggcgat atcccgcgcc gtgccgccga tatcccgcat
     6181 cgcgccgccg acatcctgca cgccgacgtc ggcgcgatcg agcgcaccaa atccggccgc
     6241 gaccgagctg gccatgccgc gttccggctg cggattcacg acgacacgca cgccgagctc
     6301 gcgcgcctga gttgcgaccg catccgcaaa cggcggcccg acgacgacga cgatatcgtc
     6361 tgtgcccgct tcgcgcgctg tcgcaacgac cttcgcgata aacgtgcggc cgtcgctacc
     6421 cgtcagcagc gcctttgcga cgccaccgag acgcgtacca gctcctgccg cgaggatcac
     6481 cgcgccgatc ttcacgcgtt tccttcgccg gcgccaatcc gctcgtaccg cacgcggccc
     6541 atggcggcgc ggtgcacagc caacccctta ttcctcacgc cagcccttcg atggccgcca
     6601 gtccccgacg ctcggcgcgc cgcggcgccg gcacgcgacc agctccgcgg cgaccgcgat
     6661 cgcgatctct tcgggcgtct cggcaccgat atcgagaccg atcggcgccc gcaatcgcgc
     6721 ccagcgctgc ttgccgaggt cgctgtcgtc gataatgccc ttcgcctcga gccgcttgcg
     6781 aaagcgtccg atcttgccgc ggctgccgat catgccgagg tagtgcagct cgtcgcggcc
     6841 gatcagctgc tcgagcagct tgagatcgat cgcgtgatcg cgcgtcacga tcaacacgta
     6901 gtcgtccgcg ccgaagccgc cgagctcgcg ctcgaccgcg gccgcatcga acgactcgac
     6961 gacgcggtcc gcccatgccg gcggctcgtc gatcgcaccg gtctcgccgt cgtcacacac
     7021 gatcaccgcg aagccgagct tcgcgagctg cggtccgagc gtccgcgcga catgaccgac
     7081 gccgaacaag atcgcgcgct cggcggtgcc gagtcgctcg acgagcgaac cgtcggcaac
     7141 gagcggttga tgcgctcgtg gatcctggtc gcccggctcg cgcaccagca gcggtccacc
     7201 gtcgagcggc gtgaccagca cgagcgcctt gcgtgcgcct gcgagcttct cgatcgcctc
     7261 gcgcgacggc gcggctggcg tgatcgcgac ttccatcgag ccgccgcaac acatcgcgag
     7321 atcgcggacg agatgctggc gcacgacacg cggcgcggcg cctgccgcaa cctctttcgc
     7381 cgcgctgacg accagcgctt cgatgcggcc accaccgacc gttccccact gctcgccatc
     7441 ggccgcgacc gccatgcgtg caccgagatg ccgcggcgtc gagccgctcg cgtgggtgac
     7501 cgccgcgatc gcgacgccgc gcccaccgtc gagcgcacgc agtgcggcgg caaatacatc
     7561 cacggccatg gccgcactat agccttaggg attggccggc ccagtaggcc gcaacacgtc
     7621 ttaatgcggc cggatcgtgc cgtcgaagcg acgaaccacg acgaccgtct gctcgtcatg
     7681 atcctgctgg cgcagtgcgc gcgccatgat gccgaccgcc aggtccgcgc cgtcgctctc
     7741 gatcacggtc gcgatcgatg gatgatcgaa cgacacgaac cagatcggcg cgtcggggaa
     7801 ccagccatcg aggcgcgccg tgttgaagtc cgccgacggc gtaccgacat gcaccggccc
     7861 gaggtcgcgc agcgctgcgc gcgccgcatc gcaaagctcg tcgaacgatg cgtcga
//
LOCUS       DASUFX010000303         6420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1575634, whole genome shotgun sequence.
ACCESSION   DASUFX010000303 DASUFX010000000
VERSION     DASUFX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575634"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="VFV99_11900"
     CDS             <1..2632
                     /locus_tag="VFV99_11900"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5060059.1"
                     /translation="AIEAPTRTARLDACRLLGDLAGRALGAEWDAAERAAFALLEAAR
                     VADTAADKRGVITAMGRGFRNIWLLPFVHRRLSDRDPTIAAAALDAAGGLGFPALEEA
                     VAAYISEGAPTVLRLAAISALGRMGAVSAVDRLVPLVVGDPLEAAAALDALTEIRSPA
                     GRTNALTALDGELEPEVQIAAVRYLAELGALDVLPLLRRLARSDDAQIRIAASLASRA
                     LKAERDRDAGERFLVALSEPDRAVRSLLARRLRTLAVTDVIEQADVLLGEDAPGVVQI
                     LGEVRDPEITRYLLQLAARAELPTHVRARAIGAIEADQTWERDELAKLAHKTELDESL
                     RAAAVQAMGAFATSTELLERVADLAASPSAAIRGAVLWALQLARRPGDGSDGPAIANL
                     VEPMLDDDDAMVRRRSAYVTGNLNLDKVAPALARRCAQSEPADLRLAAYVALGELGMP
                     GVINEVVAAVRREEDPRVLGAASNALIATSPEPSTIAQLAGRATQLLTAPDARLREAG
                     AEIAGLLGGAVPATQLVPLAKDSAPSVRGAAVWALGKIADASSEKTLLAAFADDDETI
                     HERAAAGLLRLGTPDALKQAIAFIAGHGDPAARDMLAIAIHIPPAHAERLAPAIDSAL
                     AKVDADDPVFESLVRLKLATHVSQGPAAAAIDVDAEIATVFPSFPQLAKLPGFDSLVK
                     SLRTAESLFHTTGNNPDADLSPPITLWMKVLENYVHAWLGPRMAGLQRDPGSLFDYVD
                     RVIGGSWSGYQRWIEPKWRDPAEVGTAKVDVPLRSIPNAVRDLQEHRRKRLDSPLSVT
                     EWARMMVLFAVDHPSSGFRNLFKLGGKSAERTIVLAHRLHTLAAVRNLVTHRASAGAA
                     TLAAFRRGYYSAFEDLVALA"
     gene            complement(2629..3294)
                     /locus_tag="VFV99_11905"
     CDS             complement(2629..3294)
                     /locus_tag="VFV99_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060060.1"
                     /translation="MQGSIRWVVIAGLLSACQPGDIGPQDDDAAVGDSGLRVEWSSSP
                     DMWPSNTNGVRLEKARFALDNLRVIGDAGPGDPRTSTTGIDLRWDKDVAPGEIMFDDA
                     PTGLYSQVALTLDGHTTTESFRIEGKVDVGAQNYDFKIEADTPLPFNVGVNTMVSPGD
                     IATISLRVNFMHALDSIDWATVDISDGNKELDDSDPQMAMFRLKLVESFEITNGGKPG
                     GAL"
     gene            3407..5128
                     /locus_tag="VFV99_11910"
     CDS             3407..5128
                     /locus_tag="VFV99_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5060061.1"
                     /translation="MAATEHQTQEAGTAFAVHDTAHAFVVLHIEGEERSRVLDLPDGV
                     DVTFGRSRGATVTVESEKVSRMHARVRRSGDLIEVEDLGSRNGTRVNGDKIEGIVKVT
                     NGDEIAIGAILAVVGLTSGLRRTSPVADDVRGEARLVAEVDRSVRYHRPLTLGLVRCG
                     NDAVVDAMARSLRPMDLMAEDAGDDYIVILPELNRAEGQTAMDSLLDFARGAGVTAKS
                     AMAQCPEDGTTVETLIGAVRQSLRTGRSSRDNTAAPAKNEPIVLDPAMRRVYTLVERI
                     ADTPMTILILGETGVGKEGVAEAVHKKSGRREKPLIKLNCAALPETLLESELFGYERG
                     AFTGADKRKVGFFEAADGGTLFLDEIGEMPLSLQAKLLRVLERKVITRVGGTTEIATN
                     ARVVAATHRDLEADVRAGRFRQDLMYRIGGFTIVVPPLRDRLAEIMPLAEHFARITAQ
                     EQGRPAPTLAEDARDALSGYTWPGNVRELKNAIERALVLCGEQITAADLPEKLRDARQ
                     RVAPVTPAADMRGHLAEVERAAIVTALEAEEQNQTRAARRLGLSRRALIYKMEKYGLK
                     PPPSRTD"
     gene            5329..>6420
                     /locus_tag="VFV99_11915"
     CDS             5329..>6420
                     /locus_tag="VFV99_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060062.1"
                     /translation="MFGRKSKIRKAVKRAIAGVEAENDLADYAPIEALPPDMHAQAFG
                     WTALALFDDERYRPAHHSLERALALAPDEVDLNELAAQIATEMGDIGEAIVAQQRVVA
                     ARPRDMHRIAALADMMISAERIADVIGLLQPLRHLEDPMLEAKLAESLYLSGKHQQAL
                     AILDPICAHYDSQLKQLSAADWQALKSRADDASRLRDSIYAEVHGHEATIELHAKEGK
                     LDASAGVNYKLLGARLAVSSPRIAGVLELEDPDTTERRGRVVLTHDPQAATGLVLVGI
                     AQLRRGEVGEARKTFERACEADGRCFAAFLGLGAALDCDKHHSHRRALQFALPAKLPS
                     ELERVVPDFGVLTDAERRIVWASVQPFAKL"
BASE COUNT         1097 a         2210 c         2083 g         1030 t
ORIGIN      
        1 tgcgatcgag gcgccgaccc gcactgcacg tctcgatgcg tgccggttgc tcggcgatct
       61 cgcgggccgt gccctcggcg ccgaatggga cgcggccgaa cgcgcagcat tcgcactgct
      121 cgaagcggcg cgcgtcgccg acaccgcggc cgacaagcgc ggcgtcatca ccgcgatggg
      181 ccgcggattc cgcaacatct ggctcttgcc gttcgtgcac cgccggctat cggatcgcga
      241 tccgacgatc gcggccgcgg cgctcgacgc agcgggcggc ctcggcttcc cggcgctcga
      301 agaagcggtc gccgcgtaca tcagtgaggg agcaccgacg gtgctgcggc ttgccgcgat
      361 cagcgcgctc ggtcgcatgg gtgcggtcag cgccgtcgat cgcctggtgc cgctcgtcgt
      421 cggcgatccg ctcgaagcag ccgcggcgct cgatgcactc accgagattc gctcgccagc
      481 aggacgcacg aacgcgctca ccgcgctcga cggcgagctc gagcccgagg tgcagatcgc
      541 ggcagtgcgc tatctcgccg agctcggcgc actcgacgtg ttgccgctgc tccgccggct
      601 cgcgcgatcg gatgacgcac agatccggat cgccgcgagc ctggcttcgc gcgcgctcaa
      661 ggcagaacgc gatcgtgacg ccggcgaacg cttcctcgtc gcactcagcg agcccgaccg
      721 cgccgtgcgg tccttgctcg cgcgccggct gcgcacgctc gccgtgacag acgtgatcga
      781 gcaagccgac gtgctgctcg gcgaggacgc gcccggcgtc gtgcagattc tcggcgaggt
      841 gcgcgatccg gagatcacgc gctacttgct ccagctcgct gcacgcgcgg agctgccgac
      901 gcacgtgcgg gcgcgtgcga tcggtgcgat cgaagccgat caaacgtggg agcgcgacga
      961 gctcgcgaag ctcgcgcaca agaccgagct cgacgagtca ttgcgtgcgg cggccgtcca
     1021 ggcgatgggg gcgttcgcga cgagcaccga gctactcgag cgcgtggccg atctggcggc
     1081 atcgccgtcg gccgcgatcc gcggcgccgt gttgtgggcc cttcagctcg cgcgccgtcc
     1141 aggtgacggc agcgacggtc cggcgatcgc aaacctcgtc gagccgatgc tcgacgacga
     1201 cgacgcgatg gtgcgccgcc gctccgcgta cgtcaccgga aacctcaatc tcgacaaggt
     1261 cgcccctgcc ctcgcgcgtc gctgcgcaca gagtgagccc gcggatctgc gcctcgccgc
     1321 gtacgtcgca ctcggcgagc tgggcatgcc cggcgtgatc aacgaggtcg tcgccgcggt
     1381 ccgccgcgaa gaagatccgc gcgtgctcgg cgccgcgtcg aacgcattga tcgcgaccag
     1441 ccccgaaccc agtacgatcg cgcaacttgc cggccgcgcc acccagctgc tcaccgcacc
     1501 cgacgcgcgc ttgcgtgaag cgggcgccga gatcgcgggc ctgctcggcg gcgcggtgcc
     1561 ggcgacgcag ctcgtgccgc tcgcgaagga cagcgcacca tcggtgcgtg gcgccgcagt
     1621 gtgggcgctc ggcaagatcg ccgacgcatc gagcgaaaag acgctgctgg cggcgtttgc
     1681 cgacgacgac gagaccatcc acgagcgcgc cgccgccggt ctactccggt tgggtacgcc
     1741 cgatgcgctg aagcaagcga tcgcgttcat cgcggggcac ggcgatccgg ccgcgcgcga
     1801 catgctcgcc atcgcgatcc acattcctcc cgcgcatgcc gagcggctgg cgccagcgat
     1861 cgacagcgca ctcgcgaagg tagatgccga cgatccggtg ttcgagtcgc tggtgcgact
     1921 caagctcgcg acgcacgtgt cgcaaggtcc ggccgccgct gccatcgatg tcgacgccga
     1981 gatcgcgacc gtgttcccga gcttcccgca actggcgaag ctcccgggct tcgactcgct
     2041 cgtgaagagc ttgcgcaccg cggagtcgtt gttccacacg accggcaaca acccggatgc
     2101 ggatctgtca cctccgatca cgctgtggat gaaggtgctc gagaactacg tgcatgcgtg
     2161 gcttggcccg cgcatggcgg gtctgcagcg cgatcctgga tcgctgttcg actacgtcga
     2221 tcgcgtgatc ggcggcagct ggagcggcta tcagcgctgg atcgagccga agtggcgcga
     2281 cccggcggaa gtcggcaccg cgaaggtcga cgtgccgctg cgctcgatcc cgaacgcagt
     2341 gcgcgatcta caggaacatc gccgcaagcg cctcgactcg ccgctatccg ttaccgaatg
     2401 ggcgcgcatg atggtgctat tcgccgtcga ccatccgtcg agcggctttc gcaatctgtt
     2461 caagctcggc ggcaagagtg cggaacgcac gatcgtgctc gcgcatcgtc tgcatacgct
     2521 cgccgcggtg cgcaacctcg tcacgcaccg cgcgagtgcc ggcgcagcga cgctcgccgc
     2581 gttccgccgc ggctattact cggcgttcga agacctcgtc gcgctcgctt agagcgcgcc
     2641 gcccggcttg ccgccgttcg tgatctcgaa gctctcgacc agcttcaggc gaaacatcgc
     2701 catctgcggg tcactgtcgt ctagctcctt gttgccgtcg ctgatgtcga cggtcgccca
     2761 gtcgatgctg tcgagggcat gcatgaagtt gacgcgcagg gagatcgtcg cgatgtcacc
     2821 cgggctcacc atcgtgttga caccgacatt gaagggcagc ggcgtgtcag cctcgatctt
     2881 gaagtcgtag ttctgggcgc cgacatcgac cttgccttcg atccggaacg attccgtggt
     2941 cgtgtggcca tcgagcgtga gcgcgacttg cgagtacagc ccggtcggag cgtcgtcgaa
     3001 catgatctcg cccggcgcga cgtccttgtc ccagcgcagg tcgatgcccg tggtcgacgt
     3061 acgaggatct ccggggccgg cgtcgccgat cacgcgcagg ttatcgagag cgaagcgcgc
     3121 tttctcgagc ctcacgccgt tcgtgttgct gggccacatg tccggcgagc tggaccactc
     3181 gacccgcaac cccgagtcac cgaccgcagc gtcatcgtcc tgtgggccga tgtcgcctgg
     3241 ctggcacgcg gagagcagtc cggcgataac cacccaccgg atcgagccct gcatggggaa
     3301 cgatgttagc aaccgcaagg ccacgacgaa acagaaacaa catcgcgaat tcccgcccaa
     3361 caaggtgcac gcaacttgcc catctgtgca cgatccgtac aaagcaatgg cagcaactga
     3421 gcatcagacc caggaagccg gcaccgcatt cgcagtccac gatacagcgc atgcattcgt
     3481 cgtcctccat atcgaaggcg aggagcgctc acgcgtactc gaccttcctg atggtgtgga
     3541 tgtcacattc ggccgctcgc gcggcgccac ggtgaccgtc gagagcgaga aggtctcgcg
     3601 catgcacgct cgcgtgcggc gcagcggtga tctgatcgaa gtcgaggatc tcggctcgcg
     3661 caacggcacg cgcgtcaacg gcgacaagat cgaaggcatc gtcaaagtca cgaatggcga
     3721 cgagatcgcg atcggcgcga tcctcgcggt cgttggcctg acgagcggcc tacgtcgcac
     3781 gagcccggtc gcggacgacg tgcgcggcga agcacggctg gtggccgagg tcgatcgctc
     3841 ggttcgctat caccgcccac tcacgctggg cctggttcgc tgcggaaacg atgccgtcgt
     3901 cgacgcgatg gcgcgctcgc tgcgcccgat ggatctgatg gcggaagacg ccggcgacga
     3961 ctacatcgtg atcttgcccg agctcaatcg tgccgaaggc cagaccgcga tggacagctt
     4021 gctcgacttc gcacgcggcg cgggcgtcac cgcgaaatcg gcgatggccc aatgccccga
     4081 agacggcacc acggtcgaga cattgatcgg tgcagtgcgc cagagcctgc gtaccggccg
     4141 cagctcgcgc gataacaccg ccgcgccggc gaagaacgag ccaatcgtgc tcgatccggc
     4201 gatgcgccgc gtgtacacgc tcgtcgagcg catcgccgac acgccgatga cgatcttgat
     4261 cctcggcgag accggcgtcg gcaaagaagg cgtcgccgaa gcggtccaca agaagtcggg
     4321 ccgccgcgag aagccgctca tcaagctcaa ttgcgccgcg ttgcccgaga cgctgctcga
     4381 aagcgagctg ttcggctacg aacgcggtgc gtttaccggt gccgacaagc gcaaggtcgg
     4441 cttcttcgag gccgccgatg gcggcacgct gttcctcgac gagatcggcg agatgccgct
     4501 ctcgctgcag gcgaagctgc tccgtgtcct cgagcgcaaa gtgatcacgc gcgtaggcgg
     4561 tacgacggaa atcgcgacga acgcgcgcgt cgtcgccgcg acccaccgcg atctcgaagc
     4621 cgacgttcgc gccggccgct tccgccagga tctgatgtac cggatcggcg gcttcacgat
     4681 cgtcgtaccc ccgctgcgcg atcgtctggc cgagatcatg ccgctcgccg agcacttcgc
     4741 ccggatcacc gcgcaagagc aaggccgccc cgcaccgaca ctcgccgagg atgcccgcga
     4801 cgcgctctcc ggttacacgt ggccgggcaa cgtgcgcgag ctcaagaacg cgatcgagcg
     4861 cgcgctcgtg ttgtgcggcg agcagatcac cgccgccgac cttcccgaga agctgcgcga
     4921 tgctcgccag cgcgtcgcgc cggtcacacc cgccgccgac atgcgcggcc atctcgcaga
     4981 ggtcgaacgc gccgcgatcg tcaccgcgct cgaggccgaa gaacagaacc agacccgtgc
     5041 cgctcgccgg ctcggcctgt cgcgtcgcgc gctgatctac aagatggaaa agtacggcct
     5101 aaaacccccg ccttctcgca cagattgacg accggaatcg gcgcgtctag cgcgcatcta
     5161 acgcgtcagc atcgccctcg gtggcttaca agttcgcccc gccctcatgt ccgcgttcgt
     5221 ctcgcgttcc ctccatctac ctccgcggtg aggttcgcat gcgagtcttc accggtgttt
     5281 tgactgcaac aagtagatgc ggcatgttgc cggaccaacg agccgttgat gtttgggcgc
     5341 aagtcgaaga tccgcaaagc ggtcaaacgt gcgattgccg gtgtcgaggc cgagaacgat
     5401 ctggccgatt acgcgccgat cgaggcgctg ccacccgaca tgcatgcgca ggcattcggc
     5461 tggacagcgc tcgcattatt cgacgacgaa cgctaccgcc cggcacacca cagcctcgaa
     5521 cgcgcactcg cgctcgcgcc cgacgaagtt gacttgaacg agctcgccgc tcaaatcgcc
     5581 accgaaatgg gcgacatcgg tgaggcgatc gtcgcgcagc agcgtgtggt cgcggcccgt
     5641 ccgcgtgaca tgcatcgcat cgcagcgctt gctgacatga tgatctccgc agagcggatt
     5701 gccgatgtga tcgggctgct gcagcccctt cgtcatctcg aggatcccat gctcgaggcc
     5761 aagctcgccg agtctctcta cctctcgggc aagcaccaac aggcgctcgc gatcctcgat
     5821 ccgatctgtg cgcattacga tagtcagctc aagcaactgt cggccgccga ttggcaagca
     5881 ctcaagtcgc gcgccgacga tgcctcgcga ctgcgcgata gcatctacgc cgaggtgcat
     5941 ggccatgagg ccacgatcga gctccacgcc aaagaaggta agctcgacgc gagtgcaggc
     6001 gtcaattaca agctgctcgg cgctcgcctc gcggtctcca gtccgcgtat tgccggcgtg
     6061 ctagagctcg aggatccgga cacaaccgaa cgacgtgggc gtgtagtcct cacgcacgat
     6121 ccgcaagccg ccaccggctt ggtgctcgtc ggcatcgcgc agttacgccg cggcgaggtc
     6181 ggggaagcgc gcaagacatt cgaacgcgcg tgcgaagccg atggccgctg ctttgccgca
     6241 tttctaggac tcggtgcggc actcgactgc gacaagcatc actctcaccg gcgagcattg
     6301 cagtttgcgc tgccggcgaa attgccctcc gagcttgagc gcgtggtgcc cgatttcggc
     6361 gtactgacgg acgccgaacg ccgcatcgtg tgggcatcgg tgcagccgtt cgccaagttg
//
LOCUS       DASUFX010000304        10281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_741453,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000304 DASUFX010000000
VERSION     DASUFX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741453"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VFV99_11920"
     CDS             complement(<1..1728)
                     /locus_tag="VFV99_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5060063.1"
                     /translation="MLKRGVCILLLVCAWSLDAAAAITMTPNPLDAGTTTIGNTTMDT
                     GTLDTTMNAHVDLNIGACSGGGGTFTLNPNNNINLNGPQTITVTYSPNQRGTRQCTVD
                     VVDHGNSMKLGSFTIRGTGQQPPTMGTVGTPDFGSMRWNNAAPNHTSTRNFTVSNSGD
                     VTLDVTGVTITGTNAGDFTISAAPANNSILPGNSKTWTLQFDPSASGTRTATLTFTSN
                     DPADGTDTFNLTGVGTNAIIVTPTDSNFGGLVSAGSSALGDVQVDNQGGTPEGPLGVT
                     SASISGGGGWITFAGCGGGANCLFIPALSFTGSVNVGVRCSPPATAQANDMQTATITF
                     NSDTDNATPDNSATLTCVAGRSVLAANVNNLGFGSQLVGTTTALMPLTVMNTGNQPTT
                     FVLTKTGVNAGAFAVSTPSGCGTMATPCSVAAGGTQTVNVVFTAIGEGDINAGLTITS
                     PSTPNPSLVLTGRGIDRHADVIQSLQFPDTFRNPGDMATVLPIPVKNVGEYPLHVSSV
                     AIDGAGNWALAEPFEAFDVPGLGSHDVNVAFLPVTAGKAPDGTLAIMTDDTKAALVNV
                     IVSGNGKDRN"
     gene            2046..3203
                     /locus_tag="VFV99_11925"
     CDS             2046..3203
                     /locus_tag="VFV99_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5060064.1"
                     /translation="MHRVLLVCLLAGCSLGGVTNSGDDTPGGGDDDGSGHGSGSGSGS
                     GSGSGDPATGVTCTEQNGITGLAKWLECVPAGMEGKTGTPAALVIALHGYTQTAEEFK
                     NTTEWHLLAGRYHFYVAFPQTNNDLVNAGGRPAAWKWWRDYSAWTRTSYNQHFAPINA
                     VVDKMKMAHDIDPDRVFITGLSAGGYMTTLMLACFPDIYAAGAAFSGGPHNCDLKCTD
                     SNKQQDWTRPAGYVVPSAADVKNAYSSWWNDATKRKPRVIEFHGALDQAVKPINLGDT
                     MRQWTGALGIDQTPDNASLGLPTQLGGYEYKVYAQNGQAAVATVLMSDLGHGTPVKPG
                     TAIDEGGTDPYPTQVAADCSPVSDPNCHQDWTNTGSIYGPYWAAKFFGLAP"
     gene            complement(3200..3763)
                     /locus_tag="VFV99_11930"
     CDS             complement(3200..3763)
                     /locus_tag="VFV99_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060065.1"
                     /translation="MKTLLALLVVTGCVAGSDEPSAGGGGGGGKADDGTVDGGTPDAP
                     VLTELTGSCTVTATAFHNNYGYPQTSHEPTELSLSIDEEKADGTVDGTVHVASPWGQI
                     PDGLWRGWCHLAHQDFQPADSPCAIGPYTIKTGALGFGGGFVAFRHVDDAGGGLSLYV
                     SASVESGRDSYFDPDYTRTTFSCSLAN"
     gene            complement(3923..4960)
                     /locus_tag="VFV99_11935"
     CDS             complement(3923..4960)
                     /locus_tag="VFV99_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5060066.1"
                     /translation="MRVLCVLIVMFAATTAAAQPVSFQFRSVVDVGEKPQVKLTAAQK
                     VTDIRLDLERDDGKKFALKHAGLAKGQAVTLPIGDGAAGKAKYTGTISANVPGASKWS
                     EQINFETMVRSSLKVAYDADHLDLDKHVLRFKPSRSVESAELTVIGEDGKELGTGTAS
                     YDKPPSDGWHSITWTQPENTRVMIMKLHVVGDETTATNLELIPWSVEIAHEDVNFSTD
                     SAVIEPGEDKKLDASLAKINDVIKRSEKFMKMRLYVAGHTDTVGANAKNRKLSLDRAR
                     AIAAYFRKKGIKLPIAFAGFGEEVLKVKTADNTDERANRRADYVLGPAGGAPPFKGPY
                     LKVKVGWNQLK"
     gene            5101..6198
                     /locus_tag="VFV99_11940"
     CDS             5101..6198
                     /locus_tag="VFV99_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060067.1"
                     /translation="MLCLAFAAGCAGDPDGELVCNGADLQTDPLNCGECGASCGGGAC
                     VMGQCEAATLVTGQDKPTGVAVDGAHIVWTTEGNQQGDPNGTIAECPVAGCPGMQPEK
                     VLYRGLGRPANPILVADKIYFVDGVLGTAGGDITGFVPSVGTITGAALGPPLMWWGSK
                     RDVTSLAVADNQIYFGTLEQYDGSVDAFAATCAATGCPNNGTEVYMLADARKSISSIV
                     VDKTFAYYIEFGNATIAKVSRASGGPQALFTAELGALYLAQDDTYLYWGTSVPAQSGV
                     TVKNYLARGPKAGGAKEKLADVGAITSIAVDGDWVYFADTSGLVARVAKVGGPVETLA
                     TAQKDPRGLAVAGGFLYWANHFGGSVHRLRI"
     gene            6235..7089
                     /locus_tag="VFV99_11945"
     CDS             6235..7089
                     /locus_tag="VFV99_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5060068.1"
                     /translation="MALRGYYLGCPGWGMKSWIGRLFPAGTKQADFLARYAEVFNTVE
                     GNTTFYALPSADSVARWNEQTPLHFRFCFKFPRTITHDKLLVECAAEVAEFLERVAPL
                     EHKLGTLMLQLPPRFGPGELGRLATFLDALPASFRYAIELRHDLFFAGGVEEDDVVAL
                     LRERAIDLVMMDARGLHSGKSLAQADVRARKPNLPVIMRTTGTRPIVRCVPHESFDEG
                     RRFVEPWAPQVAKWIADSKEPYFFMHAPDDTFAPENAYAFHAMLRAHADVGELPPWPG
                     EPRQLDMF"
     gene            7327..8601
                     /locus_tag="VFV99_11950"
     CDS             7327..8601
                     /locus_tag="VFV99_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5060069.1"
                     /translation="MTANIAASVESVRVIEEAEVSLLLHVRAYNRGEQAEFATQLHQL
                     LGEAERYATSPEEKAVLAEANVAVGRYLALSRDPNASPAGIKRYETEAIDALELLGDL
                     DVAQTRAAQAQAIRWDRIAEIAAIVLGVSIVGITIALVLWLRRRVIKPLFGLADTMKR
                     FGEGERSVRADESGPSELRDMTHSFNEMAEAIAVQRQAQTAFLGGVAHDLRNPLTAMR
                     LATDMLCLDEKLPPEPQVRKTINLLGRQIANLERMVGDFLDMAKIEAGALELTIAEHD
                     IRNIAREAYELFVGTSRERFHLEMPEEPVMVACDAVRIGQAVANLISNAIKYSPPEEP
                     IDIVVATVGNDVSIAVSDRGVGISADERRRLFEPFRRGATRNTIPGTGLGLYNVQRIV
                     QAHQGRIEVDSVPSVGSTFRIYLTRAAAAAAS"
     gene            complement(8602..9729)
                     /locus_tag="VFV99_11955"
     CDS             complement(8602..9729)
                     /locus_tag="VFV99_11955"
                     /inference="COORDINATES: protein motif:HMM:NF038131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor K domain-containing protein"
                     /protein_id="HEX5060070.1"
                     /translation="MGACSSTFDSVQQQVADPAPVLSITSATFTSVVGGSSISFCGQM
                     GWPGCLAPPPTQVRWGTPAGPTEQSGLGFAAGASHTIVYGDSFAIGSLHHFNFPTNSG
                     TSASGVSLDLHLRVDPSSPGPALFDSTITIPFTIDETPNQTPCAYPSTTPCADKITFG
                     TSTFALNSTADNTVYDLQILGFVDSSASTPVSGLISDENGTTSASLMAVVNQHCVDAD
                     DDGVCDETDNCVGTPNADQADSDGDGTGDACDACPLDPANDADGDGLCANVDNCPTVS
                     NGDQADTDGDGIGDACDDDNDNDGVPNPDDDCPGTTSGPVDANGCSVDQLCPCAGPWN
                     NHGQYVSCVAHVTQDFRDAGLLTSQQRSKLVSNAGQSDCGK"
     gene            complement(10013..>10281)
                     /locus_tag="VFV99_11960"
     CDS             complement(10013..>10281)
                     /locus_tag="VFV99_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5060071.1"
                     /translation="PKPRTKPVESHPTLVVRVDAAGRPSIGGNVIDEATLDELLRMSA
                     ADDRDTEVVVKAAPGAPHKSVVFVLEHAKAAGLHRMAIGTDDAP"
BASE COUNT         1751 a         3307 c         3392 g         1831 t
ORIGIN      
        1 gttgcggtcc ttgccgttgc ccgacacgat cacattgacc agcgccgctt tcgtgtcgtc
       61 cgtcatgatc gcgagcgtgc cgtccggcgc cttaccggcg gtgaccggca ggaatgcgac
      121 gttgacgtcg tggctaccga ggccgggaac gtcgaacgcc tcgaacggct cggcaagcgc
      181 ccagttgccc gcaccgtcga tcgcgacgga cgagacgtgc agcggatact cgccgacgtt
      241 cttgaccgga atcggcagca cggtcgccat gtcacccgga ttgcggaacg tgtcggggaa
      301 ttgcagcgac tggatcacgt cggcatgacg gtcgatgcca cggccggtga gcacgagcga
      361 gggattcggt gtcgacgggc tcgtgatggt aagacccgcg ttgatgtcac cttcgccgat
      421 cgcggtgaag acgacgttga cagtctgcgt gccgccggcc gccaccgagc acggagtcgc
      481 catggtgccg cagcccgacg gcgtgctgac ggcgaatgcg cccgcgttca cgccagtctt
      541 cgtcagaacg aacgtggtcg gctggttacc ggtgttcatg accgtcagcg gcatgagcgc
      601 cgtcgtggtg ccgacgagct gcgagccgaa gccaaggtta ttgacgttcg ccgcgagcac
      661 gctccggccg gcgacgcacg ttagcgtggc cgagttgtcc ggggtcgcgt tgtcggtgtc
      721 gctattgaac gtgatcgtag ccgtctgcat gtcgttcgct tgcgcggtcg ccggcgggct
      781 gcaccgcacg ccgacgttga ccgagccggt gaacgagagc gccggtatga acaggcagtt
      841 cgctccgcca ccacagccgg caaacgtgat ccagccaccg ccgccactga tcgatgcgct
      901 ggtgacgccg agcggaccct cgggcgtacc gccttggttg tcgacctgca cgtcgccgag
      961 cgcagacgag cccgcgctga cgaggccacc gaagttcgaa tcggttggcg tcacgatgat
     1021 cgcgttcgtg ccgacgccgg tcaggttgaa cgtgtcggtg ccgtcggcag ggtcgttgct
     1081 cgtgaacgtc agcgtcgcag tgcgcgtgcc gctcgcgctc ggatcgaatt gcagcgtcca
     1141 ggtcttcgag ttaccgggca ggatcgagtt attcgccggc gcagccgaga tcgtgaagtc
     1201 accggcgttc gtgcctgtga tcgtcacgcc ggtcacgtcg agcgtgacgt cgccgctgtt
     1261 cgagacggtg aagttgcgcg tcgacgtgtg attcggcgcg gcgttgttcc agcgcatcga
     1321 gccaaagtcg ggcgtgccga ccgtgcccat cgtcggcggc tgctggccgg tgccgcggat
     1381 cgtgaacgag ccgagcttca tgctgttgcc gtggtcgacg acgtcgaccg tgcattggcg
     1441 cgtaccgcgc tggttcggcg agtacgtaac tgtgatcgtc tgtggaccgt tcaggttgat
     1501 gttgttgttc gggttgagcg tgaacgtacc gccgccaccg gagcaggcgc cgatgttgag
     1561 atcgacgtgg gcgttcatcg tcgtgtcgag cgtgcccgtg tccatcgtgg tgttgccgat
     1621 ggtcgtcgta cccgcatcga gcgggttcgg ggtcatcgtg atcgcggcgg ccgcgtccag
     1681 cgaccacgcg cagaccagta gcaggatgca cacccctcgc ttcaacactg caacgacgat
     1741 ggtaccgagc agcgtgcgta aaccgcaata cttcgcaact agcaggtgat cacgacaatc
     1801 tcggagatcc tggcacttac acgccctctc catagcagag cgatgtcgag cgtgttgagt
     1861 gtgatctgag tcgtcggtcg catgtaggca gcaatgacac cacgagcctg gtcggatcat
     1921 ttgctcgagc catcacacga gggcgatgcc tgttggctgg agccaatgat cgcgctcgcg
     1981 gatcctgcat cgaacatccg tttcgcggca cgaacgcggc acatgcgttg cttcgagcag
     2041 cgctcatgca tcgtgtcctg ctcgtgtgcc tgctcgctgg ttgctcactc ggtggcgtga
     2101 cgaattcggg agacgacaca ccgggcggcg gcgatgacga cggcagtggg cacggcagtg
     2161 gcagcggtag cggcagtgga agtggcagtg gcgatccggc gacgggcgtc acctgcactg
     2221 agcagaacgg catcaccggc ctcgcgaaat ggctcgagtg cgtgccggcc ggcatggaag
     2281 gcaagaccgg cacgcctgct gcgctcgtca tcgcgttgca cggctacacg cagaccgccg
     2341 aggaattcaa gaacacgacc gagtggcatc tgctcgccgg gcgctaccac ttctacgtcg
     2401 cgtttccgca gacaaacaac gatctcgtca acgcgggagg acgtcccgct gcctggaaat
     2461 ggtggcgtga ttactcggcg tggacgcgca cgagctacaa ccagcacttc gctccgatca
     2521 atgccgtcgt cgacaagatg aagatggcgc acgacatcga tccggatcgc gtgttcatca
     2581 ccggactctc ggccggtggc tacatgacga cgctgatgct cgcgtgcttt cccgacatct
     2641 acgcggccgg tgcggcgttc tcgggcggcc cgcacaactg cgatctcaag tgcaccgaca
     2701 gcaacaagca gcaagactgg acgcggcccg cgggctatgt cgtgccgagt gccgccgacg
     2761 tcaagaacgc ctactcgtcg tggtggaacg acgcgacgaa gcgtaagccg cgtgtgatcg
     2821 agttccacgg cgcgctcgat caggcggtca aaccgatcaa cctcggcgac acgatgcggc
     2881 agtggaccgg tgcgctcggc atcgatcaga cacccgacaa cgcgagcctc ggtctaccga
     2941 cgcagctcgg cggttacgag tacaaggtct acgcgcagaa cggccaggcg gccgtcgcga
     3001 cggtactgat gagcgacctc ggccacggca ccccggtgaa accgggcacc gcgatcgacg
     3061 aaggtggcac cgatccgtat ccgacgcagg tcgcggccga ttgcagcccg gtttccgatc
     3121 cgaactgtca ccaggactgg acgaacaccg gatcgatcta cggaccctat tgggccgcga
     3181 agttcttcgg cctggcgcct tagttcgcga gcgagcagga gaacgtcgtg cgcgtgtagt
     3241 cgggatcgaa gtacgagtcg cgaccgctct cgacgctcgc gctgacgtag agcgagaggc
     3301 cgccgcccgc gtcgtcgacg tgccggaacg cgacgaagcc gccgccgaag ccgagcgcgc
     3361 ctgtcttgat cgtgtacgga ccgatcgcgc acggcgaatc ggccggttgg aagtcttgat
     3421 gcgcgaggtg gcaccagccg cgccacaggc catcggggat ctggccccat ggcgacgcga
     3481 catgcacggt gccgtcgacg gtgccgtccg ccttctcttc atcgatcgag agcgagagct
     3541 cggtcggctc gtgcgacgtc tgcgggtagc cgtagttgtt gtggaacgca gtcgccgtca
     3601 ccgtgcagct gccggtgagc tcggtcagca ctggcgcgtc gggcgtgccg ccatcgacgg
     3661 tgccatcgtc tgccttgccg ccaccgccgc cgccgccggc agacggctca tcgctgccag
     3721 caacacaacc ggtgaccact aggagcgcga gcagtgtctt catggccgcg ccatcgagca
     3781 tccggcgtgc cgcgccaagt catcagcaat ccacgaccac accgtggcag ctcgagtcgc
     3841 cagggcaggt ctagtagccg aagttcctga gttgcagact ttcggttgca gcgagctgga
     3901 gcccctcgac gtcgggtaaa cgtcacttca actggttcca gcccaccttg accttgagat
     3961 acgggccttt gaacggaggc gcgcccccgg cggggccgag cacgtagtcg gcgcggcgat
     4021 tggcccgctc gtcggtgttg tcggccgtct tcaccttgag cacttcttcg ccgaagccgg
     4081 caaacgcgat cggcagcttg atgcccttct tgcggaagta cgctgcgatc gcgcgcgcgc
     4141 gatcgagcga gagcttccgg ttcttcgcgt tcgcaccgac ggtgtcggtg tggccagcga
     4201 catagaggcg catcttcatg aacttctcgc tgcgcttgat gacgtcgttg atcttcgcga
     4261 ggctcgcgtc gagcttcttg tcttcacccg gctcgatcac cgcgctgtcg gtactgaagt
     4321 tgacgtcctc gtgcgcgatc tcgacggacc acggaatcag ctcgaggttg gtcgccgtcg
     4381 tttcatcgcc gacgacgtgt agcttcatga tcatgacgcg cgtgttctcg ggctgcgtcc
     4441 acgtgatcga gtgccagccg tcgctcggtg gtttgtcgta gctcgcggtg ccggtgccga
     4501 gctccttgcc gtcctcgccg atcacggtga gctccgcgct ctcgaccgaa cgcgatggct
     4561 tgaaccgcag cacgtgcttg tcgagatcga ggtggtcggc gtcgtacgcg accttgagcg
     4621 acgagcgcac catcgtctcg aagttgatct gctcgctcca cttgctcgcg ccggggacgt
     4681 tcgccgagat cgtacccgtg tacttcgcct tgccggcggc gccatcgccg atcggcagcg
     4741 tgactgcttg ccccttcgcg aggcccgcgt gcttgagcgc gaacttcttg ccgtcgtcgc
     4801 gttcgaggtc gagccgaata tcggtgacct tctgtgccgc ggtgagtttc acctgcggct
     4861 tctcgccgac gtcgacgacc gaacgaaact ggaagctgac cggctgtgcc gcggccgtgg
     4921 tggcagcaaa catcacgatc aagacgcaga gcacccgcat ggcaccgagc atagcgcgcg
     4981 atgtcgcggt cgttgtgaac agcggttcat accgacggaa accgcgccga aatcgcccgc
     5041 aaaaaacgtc ccgttcctgg cagcggctgc gtcttggaca gcatgcgatc gagtccaatc
     5101 ttgttgtgtc ttgccttcgc cgcgggctgc gccggcgacc ccgacggcga gctcgtttgt
     5161 aatggtgccg atctacaaac cgacccgctg aactgtggcg agtgcggcgc gagctgcggc
     5221 ggtggcgcgt gcgtgatggg ccagtgcgag gccgcgaccc tcgtcaccgg ccaggacaag
     5281 ccgaccggtg tcgccgtcga cggcgcgcac atcgtgtgga ccaccgaggg caaccagcag
     5341 ggcgatccga acggcacgat cgcggagtgc ccggtcgccg gctgcccagg catgcagccg
     5401 gagaaggtgc tctatcgcgg gctcggtcgg cccgcgaatc cgatcctcgt cgccgacaag
     5461 atctacttcg tcgacggcgt gctcggcacc gccggcggtg acatcacggg cttcgtgccg
     5521 tcggtgggca cgatcaccgg cgccgcgctc gggccgccgc tcatgtggtg gggcagcaag
     5581 cgcgacgtca catcgctcgc agtcgccgac aatcagatct acttcggtac gctcgagcaa
     5641 tacgacggtt ccgtcgatgc attcgcggcg acgtgcgcag caaccggctg cccgaacaac
     5701 ggcaccgagg tctacatgct cgccgatgcg cgcaagagca tctcgtcgat cgtcgtcgac
     5761 aagacgttcg cgtattacat cgagttcggc aacgcgacga tcgcgaaggt gtcgcgcgcg
     5821 agcggcggcc cgcaggcgtt gttcacggcc gagctcggtg cgctatacct cgcgcaggac
     5881 gacacctatc tgtattgggg aacgtcggtg ccggcgcagt cgggcgtcac ggtgaagaac
     5941 tacctggcgc gcgggccgaa ggcgggcggc gcaaaggaga agctggccga cgtcggggcg
     6001 atcacctcga tcgcggtcga cggcgattgg gtgtacttcg ccgacacgtc tggcctggtc
     6061 gcccgagtcg cgaaggtggg cggcccggtc gagaccttgg ccaccgcgca aaaggacccg
     6121 cgcggtctgg ctgtcgccgg tggcttcctc tactgggcga accacttcgg cggctcggtt
     6181 cacaggctcc gcatctgatc acagccgcgc attcgctggt agggtttcgc cgtcgtggcg
     6241 ctgcgcggct attacctcgg ttgcccgggc tggggcatga agagttggat cggccggctg
     6301 ttccctgcgg ggaccaagca ggccgacttt ctggcgcgct atgcggaggt cttcaacacc
     6361 gtcgagggca acacgacgtt ctacgcgctg ccgtccgccg attccgtcgc gaggtggaac
     6421 gagcaaacgc cgctgcactt ccggttctgc ttcaagttcc cgcgcacgat cacgcacgac
     6481 aagctgctcg tcgagtgcgc ggcggaggtc gccgagtttc tcgagcgtgt cgcgccactc
     6541 gagcacaagc tcggcacgct gatgctacag ctaccaccgc gcttcggacc gggcgagctc
     6601 ggtcggctcg cgacgtttct cgacgcgctg ccagcgtcgt ttcgttatgc gatcgagctt
     6661 cgtcacgacc tcttctttgc cggcggcgtc gaggaagatg acgtcgtcgc acttctccgc
     6721 gagcgcgcga tcgatctcgt gatgatggat gcacgcgggc tgcattcggg gaagagcctc
     6781 gcgcaagccg acgtccgcgc acgcaagcca aacctgcccg tgatcatgcg aactacaggg
     6841 acgcggccga tcgtgcgctg cgtgccacac gaatcgttcg acgaaggacg ccgcttcgtc
     6901 gagccgtggg cgccgcaggt ggcgaagtgg atcgccgaca gcaaggagcc ttacttcttc
     6961 atgcacgcac cggacgacac atttgcgccg gagaacgcgt atgcgtttca cgcgatgctg
     7021 cgcgcgcacg cggacgtcgg cgagctgccg ccatggccgg gtgaacctcg ccagctcgac
     7081 atgttctagt ttcgtaggcg ggcttcccgc aatttgcgcg tatgcggagt gagccgtgcg
     7141 gattcgccca tccggacgat tcagcgactc gacatgtcgc ccgcgttcgg tacacctcac
     7201 ttcgagatgt cagactcgcg acctgcgaat ctgcggaacg tcctgtcctt cgccgccatt
     7261 ctcgtgacgc tactggcggc aacgtcagcg atcgctctcg tgctcgcgac cagcgtactg
     7321 caacggatga cagcgaatat cgcggcctcg gtcgagagcg tgcgcgtgat cgaagaagca
     7381 gaagtcagtc tgctgctcca cgtgcgcgcg tacaaccgcg gcgagcaggc cgagttcgcg
     7441 acacagctgc atcagctgct tggcgaagcg gagcgctacg cgacgagtcc ggaggagaag
     7501 gccgtgcttg ccgaggcgaa cgtcgcagtc gggcgatatc tcgcgctcag tcgcgatccg
     7561 aatgcttcgc cggcggggat caaacgctac gagaccgagg cgatcgatgc gctcgagcta
     7621 ctcggtgatc tcgacgtcgc gcagacgcgc gcggcacagg cgcaagcgat ccgctgggat
     7681 cgaatcgcag agatcgccgc gatcgtcctc ggtgtttcga ttgtcggaat cacgatcgcg
     7741 ctcgtgctgt ggctgcgccg ccgcgtgatc aagccattgt tcggcctcgc cgacacgatg
     7801 aagcggttcg gcgagggtga acgctccgtg cgcgcggacg agagtggccc gagcgagctc
     7861 cgcgacatga cccacagctt caacgaaatg gcagaggcga tcgcggtcca gcgtcaggca
     7921 cagaccgcgt ttctcggcgg tgtcgcgcac gatcttcgca acccactcac cgcgatgcgg
     7981 cttgcgaccg acatgctctg tctcgacgag aaactgccgc ccgagccgca ggtgcgcaag
     8041 acgatcaacc tgctcgggcg tcagatcgcg aacctcgagc ggatggtcgg cgacttcctc
     8101 gacatggcga agatcgaagc cggcgcgctc gagctcacga tcgccgagca cgacatccgc
     8161 aacatcgccc gagaggcata cgagctattc gtcgggacgt cgcgcgaacg ctttcatctc
     8221 gagatgccgg aggagcccgt aatggtcgca tgcgatgcgg tgcgcatcgg ccaggcggtg
     8281 gcgaacctca tcagcaatgc gatcaagtac tcaccgccag aagagccgat cgatatcgtc
     8341 gtcgcaacgg tcgggaacga cgtttcgatc gcggtgtcgg atcgcggcgt cggcatctcc
     8401 gccgacgaac gccgtcggct gttcgagccg ttccggcgcg gcgcgacgcg caacacgata
     8461 cccggcacgg ggcttggcct ttacaacgtg caacgcatcg tgcaagccca ccagggacgc
     8521 atcgaggtcg acagcgttcc ctcggtgggc agcacgtttc ggatttacct gacgcgtgct
     8581 gccgccgcgg cggcgagctg attacttacc gcagtcgctc tggccggcgt tcgacaccag
     8641 cttcgaacgc tgctgcgacg tcaacaggcc ggcgtcgcgg aagtcctgcg tcacgtgtgc
     8701 gacgcagctg acgtactggc cgtggttgtt ccacggaccg gcgcacgggc agagctgatc
     8761 gaccgagcag ccgttcgcgt cgaccggacc cgaggtcgta cccgggcaat cgtcgtccgg
     8821 gttcggcacg ccgtcgttgt cattgtcgtc gtcgcacgcg tcgccgatgc catcgccgtc
     8881 ggtgtcggcc tgatcgccgt tcgagaccgt cgggcagttg tcgacgttcg cgcagaggcc
     8941 atcgccgtcg gcatcgttcg ccggatcgag cgggcacgca tcgcacgcgt caccggtgcc
     9001 gtcgccatcg ctgtcggcct ggtccgcgtt cggcgtaccg acgcagttgt cggtctcgtc
     9061 gcatacgccg tcgtcatccg catcgacgca gtgctggttc acgaccgcca tcaggctcgc
     9121 gctcgtcgta ccgttctcat ccgagatgag accgctgacc ggagtcgacg cgctcgaatc
     9181 gacgaagccg agaatctgga gatcgtagac cgtgttgtcg gcggtcgagt tgagcgcgaa
     9241 cgtcgacgtg ccgaacgtga tcttgtcggc gcacggcgtc gtcgacgggt atgcgcacgg
     9301 cgtttgattc ggcgtctcgt cgatcgtgaa cgggatcgtg atcgtcgagt cgaacagcgc
     9361 ggggcccggc gacgacggat cgacgcggag gtggagatcg aggctcacac cgctcgccga
     9421 cgtgcccgag ttcgtcggga agttgaagtg atgcagcgag ccgatcgcga agctgtcgcc
     9481 gtacacgatc gtatgcgagg cacctgcagc gaaaccaaga ccactctgct ccgtcggacc
     9541 cgcgggcgtt ccccagcgga cctgcgtcgg cggcggcgcg aggcagccgg gccaacccat
     9601 ctggccgcag aacgaaatgc tcgagccacc gacgacgctc gtaaacgtcg ccgaggtgat
     9661 cgacagcacc ggcgcaggat ccgcgacctg ctgctggacg ctatcaaacg tcgaggaaca
     9721 cgcacccaac cccaaaacca acaccgctgc tttgccgcgc atgacttgtt tgcctttcgt
     9781 aagacgccgc gttgcgagta gtggcgggca cgatagcagc gcacacagga aaatccctag
     9841 acgaaaagtg cggcgaaaaa tgctctcgat ccatcgctgc gcaatctgtg taagccgcgc
     9901 tgcgattgag gtttcggcgc gccgcggcaa cgtcaccgag gtgcgatcgg ctggtggcaa
     9961 ctaccccagc gctacccgtc gccagtaccg cgcacgtgat cgctcccgcg tgctacggcg
    10021 cgtcatcggt cccgatcgcc atgcggtgca accccgctgc cttcgcgtgc tcgagcacga
    10081 agacgacact cttgtgagga gcgcccggcg ctgctttgac gacgacctcg gtatcgcgat
    10141 cgtcggcggc actcatgcgc agcagctcgt caagcgtcgc ctcatcgatc acgttgccac
    10201 cgatgctcgg tctgcccgcc gcatcgacgc gaacaacgag cgtcggatgc gactcgacgg
    10261 gtttcgtgcg tggcttcggc g
//
LOCUS       DASUFX010000305        16993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1852288, whole genome shotgun sequence.
ACCESSION   DASUFX010000305 DASUFX010000000
VERSION     DASUFX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852288"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFV99_11965"
     CDS             complement(<1..258)
                     /locus_tag="VFV99_11965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5060072.1"
                     /translation="MTEASVVTFGIPFYAGAVYLERTLRSILAQDDDRWQAYVCDDGS
                     EPGIAELVASVGGGRIRYSKNPGNIGMAANFNRCLELAETDL"
     gene            complement(255..1598)
                     /locus_tag="VFV99_11970"
     CDS             complement(255..1598)
                     /locus_tag="VFV99_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060073.1"
                     /translation="MMERAKQAWRWLDPRVTPRMILLAALFAFLVYAWPGFIGWDSRD
                     HILQARAFSFSDGHPPAVPLLVRICEIFITGPAGVLLVQAITLLVGLNMIFKRRFSPR
                     VAALCAAGVFLWPPISGAQGLIAKDGLMAGSLVIAIGLMLDERPSRHRLALLFVFFAS
                     LMRWNALAATFPPMLLLFRWSPTLRGWRRYAIALAMWFGVTAAAYGTNEMLTTTHEHL
                     WYWGHAYLDICGTLEHLGPRPDEELNEIFDGVPLLYHDNIWQRMHAIYSPVDHYHLMR
                     DAGRMFDIPANDEQRAALLRAWKRVVLAHPVEYLAYRWENFQRVIQRHRPNTWTSVYV
                     WFNVIAAPETIPELQHDAGPSRIQARLIEASIWISLTVIYWILPYFVLSFVLLPFCTK
                     RVLEVSLLLSAIGYQLQWFFLAAAPDVRYSQWMELCVTVVAVLLAPRALSVIRRR"
     gene            complement(1609..2949)
                     /locus_tag="VFV99_11975"
     CDS             complement(1609..2949)
                     /locus_tag="VFV99_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060074.1"
                     /translation="MRESTRARIILVLGGLVFLAFARPGLMTFDSVEQLAEAREGFYT
                     DCHPPAMAALWRVLDSIMPGGFLLLVLQVTTFLAGMYLVLRHAMSERSAAIVACVLML
                     FPPVANPMAFMWKDSVMAGLLMLGAGLVLSPCRWARVASLVCFCVGTAVKYNAFAATL
                     PLIVLLFEWKQGLPWLKRYALATTAWLGVTIAAMGTNSLLTDQQMHFWHSSLAIHDIA
                     GVINYDDGFTEDQVKQELAGTGLLIDTNVLQRIREVYALHDWTKLVYSDTQLWDLPIN
                     GRVPAPQAQRDAIERAWKSLVFGHPGAYLHHRWVIFLDAIGVTVKTRTAIPPRIMHWK
                     GFLTNLGLPERSRPYQNKWSNAYNWLWTHTPIYTQWLYIVLALALLPFSRGQRDVFAV
                     LASGLVVESTLFFLAPTPDYRYSHWTVVCTCVAVAMLIARRARRQPTNRAGPQL"
     gene            complement(2971..4230)
                     /locus_tag="VFV99_11980"
     CDS             complement(2971..4230)
                     /locus_tag="VFV99_11980"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX5060075.1"
                     /translation="MDKRLRRDVIWNLAPVVLLGAVGLGMNILIAAWWHAAALGIFNL
                     VSIAYFVLAVVGAWGIQYSVLRAVAEKPDDSAHKAAVVVGALVPNVVLAALATAIFFA
                     SRNLVARLHGSDEVAEGMLYATPGLFCFAVNKVLFGIVNGQRRMRAFAIYTSLRYVLI
                     AVGIVAAHVMRIDAAHLPVIWTFTEGTLLLVLIGETIATVTLARAAGWQRWAREHVAY
                     GARGVTATLAYEIQTKLDVWMVGAAGFPKAAVGIYSLAAALNEGATQLAVVVMNNINP
                     VMAQSLATGRRDEVEALVRRTRRWFVPLLAGACLVGAAAFPYIIPWVIGDASFQAGAL
                     PFAILMGGLALASPYLPFAQVLLMANRPGWHTVFVLIVVAVNFVANLLLIPVLGLAGA
                     ALAMSIAVVTVAAMLWRLAPTRAGVRL"
     gene            complement(4230..6155)
                     /locus_tag="VFV99_11985"
     CDS             complement(4230..6155)
                     /locus_tag="VFV99_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060076.1"
                     /translation="MCGIFGVICASDQKVDRDLARALATSLLRFSETRGREAAGIAVH
                     DGERIQVLKQGGSVSDFLKNPVLQQLLDTALTRFAAGNTIAITGHSRLATNGTQADMS
                     NNQPVVTHGAVALHNGIVVNDRKLAERYAIAPQSELDSEVLATLLRKKLNASGDIVAA
                     TRATFAELEGAASIAMLFDNLDVMLLATNTGSLFQLSTDAGNVIAFASERFILQRVIE
                     DKDLSSRLGDVRLEQIRAGHALAVSLATLQRRAFSLAPDRDEPPFGSAPNGHQVSIVD
                     HSSHADRLRRCTKCILPETFPFVGFDEHGVCAYCRQWKPIQPKGVEELLRAVEPYRSK
                     DGSPDVIVAFSGGRDSSYGLHYVKTQLGMNPVAFTYDWGMVTDLARRNCARVCGKLGI
                     EHIIRSADITAKRRYVRKNIEAWLKKPELGMVSLFMAGDKEFYSHARQLRKETGIKLV
                     IFCTGNLIEEARYKVGFMGVPQDDDNMVLPNMSVRNKASMLWYFAKNYAKNPAYINES
                     ILDTANAFYQTFIVKDDFLYLYHYLPWHEDEIVGTIRREYDWEVSPDTTTTWRIGDGT
                     AAFYNYIYATIAGFTEDESMLSNMVREGHLTRDEAMRRAKEYGKPRWASLREYAQLIG
                     FSFEEAMQVINAAPKLY"
     gene            6241..6984
                     /locus_tag="VFV99_11990"
     CDS             6241..6984
                     /locus_tag="VFV99_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5060077.1"
                     /translation="MRLLLIIPAYNEQEALGGLLGEIRALPSLGVDVETVVVDDGSAD
                     RTAEVAQQGGARVVRLCRNLGIGGAVQSGLRLAHREGFDFAVQIDGDGQHPPSELARL
                     LEPTRGASPPDLIVGTRYRDAANFRSTPLRRLGSWWLRVILRMLARLKVSDPTSGFRL
                     YNKRALRLFDETYPYDFPEPESLAIARAAGLRVSEVSVTMRERQGGTSSIIGFDSIYY
                     MFKVTIAVVLTYVRALGRARKKGSDAATD"
     gene            6968..7354
                     /locus_tag="VFV99_11995"
     CDS             6968..7354
                     /locus_tag="VFV99_11995"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX5060078.1"
                     /translation="MQQPTEEYYRFHGGAGNWVFAGVCLVIAIVLARLILRERISLQG
                     SMSYLAFVLVIGAMALFPEQAGAVAHALGFSTLANFFFCTCIAGLAILHLRALVTLSR
                     VHLRSVVLTQELALLQEKLDRAIEKR"
     gene            complement(7355..8584)
                     /locus_tag="VFV99_12000"
     CDS             complement(7355..8584)
                     /locus_tag="VFV99_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5060079.1"
                     /translation="MKRAALALALIACGDKAPTKLDSGLVSDAPALACSPQSGSKISM
                     RLIATTHGAALLATSPPADPRLFVVEQIGTIRVLTNDTLLATPFLDLGDDIACCGEQG
                     LLGLVFHPQYATNGTFFVFYTTGNANVVARYQVSSDPNVANKASGTIVLSIPDFASNH
                     NGGMMEFGPDGYLYIGTGDGGGGGDPQANGQNPNALLGKMLRIDVDNKAPGKEYGIPA
                     TNPFATTGGAPEVYMMGLRNPWRWSFDSTTGDMWIGDVGQDEVEELDFIPAAEQAGAN
                     LGWRMYEADRCYDGPCNPASIRMPQFEKMHSDGWCSVIGGAVYRGSCYPDLVGTYFFT
                     DYCAHVLGAAKLGAGNVVVEFPETTFVEPPAQPRAGFPSTPASLHADSRGELYLTTTE
                     CCGTQQLGGLYHLEATQ"
     gene            complement(8584..9792)
                     /locus_tag="VFV99_12005"
     CDS             complement(8584..9792)
                     /locus_tag="VFV99_12005"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5060080.1"
                     /translation="MRYVALALALLGCGGDETPSLPPACEAPVHGTSITFREVARTKS
                     AALLVTSPPNDIRRFVVERDGIIEQLTDAGISPTLFLDVSNLIAAGGEQGLLGLAFHP
                     KYASNGTFFVFYTTGDANVVARYTVSKTNPEVADPASAKVILSIPDFAGNHNGGMMEF
                     GKDGYLYIGTGDGGGGGDPMKTGQNPNALLGKILRIDVDKKESGKEYGIPSGNPYAKG
                     GGAPEVLVMGVRNPWRWSFDRTTGDLWIGDVGQDAVEELDVIPNGKAAGMNLGWSMYE
                     GANCYSQPCDPAGMAMPAIQKTHSGDAWCAVIAGQVYRGSCYPDIVGTGYVTDYCAHE
                     LVAAKQNGTALAEEKPTTHYADAQGIHDGVPPTPSSLHADARGELYLTTEQVSGSQTQ
                     GGIFHLEAGM"
     gene            9878..10939
                     /locus_tag="VFV99_12010"
     CDS             9878..10939
                     /locus_tag="VFV99_12010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5060081.1"
                     /translation="MASPTLSIVVPAYNEERNIPAFLAAMIPVLEGIGEPFEIIFVND
                     GSKDGTLGMLAAASSQDPRIKVVGLARNFGKDIALSAGLAHATGKAVIPIDCDLQHPP
                     ELVPQFVAKWREGFDMVIGVRSKRDEEGWLRRTLSQTYYKVMRSMTSVEIPPNAGDFR
                     LLDRKILDVINKMPERHRFMKGIFAWPGFKVASIEFQANVRANDTRSSWSFFKLWRFA
                     LDGLFSFSTAPLKLWTYIGLLSAGGAFIYLTITLIQKFFFGISAPGYASLLVVLLFFN
                     GLLMISNGIQGEYIARIFEEVKGRPLYVVGQTFGFDAQQPVVEKPEVVVTIAEPVPVA
                     PPKREKPPLPKPPSLPRPQ"
     gene            complement(10940..11500)
                     /locus_tag="VFV99_12015"
     CDS             complement(10940..11500)
                     /locus_tag="VFV99_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060082.1"
                     /translation="MKHSWLALLAACGGDGGGGGPGLTATTSFVVTSVTGAGMSPLDP
                     LAMQTIEMQIVFPDFAVDRGYEGDTTDCKSTMLWTFPAERTASGASAQLVQTQILDML
                     AYWDVRLQLCMTGKSSILLHSEIDVLNLAFGCFGIPASAQVRNADGYPEMTSFTATEC
                     SATILDVVNNRIVSNPSFSIDFALGQ"
     gene            11552..12883
                     /locus_tag="VFV99_12020"
     CDS             11552..12883
                     /locus_tag="VFV99_12020"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HEX5060083.1"
                     /translation="MKRAALIWLAIIVPIWLVLALCTYWEPIIRDGWGFLFFHRKYGL
                     SWTTAEAFMKNNYNANNPRLGQLYTLVMYTPGPWHVIVTPIVELGLFYVLATLALGRW
                     PSLRRTDDALVFVTIVAMVFATARSLGPLLFYRPFMGNYTVGLLLNLLWLVPYRLHAE
                     TRCMRWWWTPVMLGFGVVSGLCNEHTGPALLAAGLFALVVYWRRGERIVPCAWAGVIG
                     MAIGMVILLGAPGQHVRYAGAAARESIIDAVIQRGVGGAAWVVLALPLFLTPLLLWLV
                     PVFVARRRGSRSEQPRSQIVAALVALAMATLMVFTLLGSPRWGDRMYFASICLACAAA
                     ASWVCAHPGTLARRSTLTVNIAALVVVSAILLFMHHRLHDEFTARFAILEHAAPGTVV
                     RVPTYSQERSRYVLGDDFLVPTTRGSVAKAYQLQDIELDQRVPTTAPSDEP"
     gene            complement(12844..13296)
                     /locus_tag="VFV99_12025"
     CDS             complement(12844..13296)
                     /locus_tag="VFV99_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060084.1"
                     /translation="MSYADPPKPVPPTQEAQQKQQEENNGRVRLDEKGNKKPEPREAG
                     EWIELASPTPAKHGTEIVMVGKEAGSFSKLRFSSQGKTILRDVKVYFDDGTTKRLRID
                     KILPDGKFTVVDLKTDKRIDRIRVNTETQTKGLYAIYGSSDGAVVGTR"
     gene            13389..14540
                     /gene="add"
                     /locus_tag="VFV99_12030"
     CDS             13389..14540
                     /gene="add"
                     /locus_tag="VFV99_12030"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX5060085.1"
                     /translation="MEIPLELIQRLPKTDLHCHLDGSLRLDTVLDLARKQNIKLPTFD
                     RSELFNMLYAGEHVASLDDYLRAFDITLSVMQTEEGLERTAYELACDAWAENVRYIEV
                     RYSPLLHTRSGLRPAQVVEAVLRGLRTAKRELGIRYGVILCAIRTLSPESSLRIAELC
                     VAFKNRGVVGFDLAGSEVNNPAKVHRQAFQLVIDNNINCTAHAGESFGPDSVHQAIHK
                     CGAHRIGHGTRLVESGDLLNYVNDHRIPIEVCPSSNVQTRAANSWETHPVDFFVDYGV
                     RVTINTDNRLMSDTTCSKELYLCHKHYGWSLQTIKEIVISGFKSAFMPYREKADLVAE
                     VSRELAKYEDPRGEVTGRVGLVESGQPTSSDKATPPTPPTPPKAKLQVS"
     gene            complement(14545..14922)
                     /locus_tag="VFV99_12035"
     CDS             complement(14545..14922)
                     /locus_tag="VFV99_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5060086.1"
                     /translation="MCAIDARIDSPRPIDAPPPDADITPPQIFMTDPVSGASNVPTSQ
                     VVTIQFNEPVSGVSNTTFRLESSSVSIPGTVMGIDPFKYAFMPASALPANATITVYVD
                     AGIFDVPANNAFVPTSFDFMTAP"
     gene            15203..15997
                     /locus_tag="VFV99_12040"
     CDS             15203..15997
                     /locus_tag="VFV99_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5060087.1"
                     /translation="MAKLEREQLRGLVEPHWRRLYNFVFRLTLDRDRAERYLADVFLA
                     ATNKFDSKPAKDDEIEVWLLGIANQMLEERLPRQPEVNFDILDETLRSESTRTDVVRS
                     LSDPQRDFLLWELKQGCMTAVVNCLPPGERAAFVVCHVLKLGDDIAAKSLGITESAYK
                     VRLSRARKKVGDYLAPRCEHVNPMNPCRCPARVGTALAKGFIRSVGPVGGEVSLRKPS
                     EPYGRYGTGIGNEDVPMRDISAIYGGLPDPDPPGDLGNKLIDQLSQ"
     gene            16015..16266
                     /locus_tag="VFV99_12045"
     CDS             16015..16266
                     /locus_tag="VFV99_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060088.1"
                     /translation="MSSRDTTPDAVAVRTAVYRRMSDEQRVSIGAQMSEDARAIALDA
                     IRQRHPDYDEEQATFALFRLLLGDDLFRRVWPAAPLLRP"
     gene            16263..16823
                     /locus_tag="VFV99_12050"
     CDS             16263..16823
                     /locus_tag="VFV99_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060089.1"
                     /translation="MTRDGLQLLLQDVVGLLSAIGIPHMVVGSFASMVHGEPRTTQDL
                     DVVIDPTAEQLEQLLQRLPADRYYVDPDVARDARRRRSMFNVIDMASGWKMDLIVRKA
                     RPFSVEELQRRAKATMQGVVVDTATAEDTIIAKLEWSKAGSSERQLEDIAGILRVRGD
                     KLDRAYIERWVDELELREQWDRAQRL"
BASE COUNT         2980 a         5417 c         5572 g         3024 t
ORIGIN      
        1 gagatcggtc tcggcgagct cgaggcagcg gttgaagttc gcggccatgc cgatgttgcc
       61 tgggttcttg gaatagcgaa tgcggccacc gccgaccgat gccacgagtt ctgcgatgcc
      121 gggctcgctg ccgtcatcac agacgtatgc ctgccagcga tcgtcgtctt gcgcgaggat
      181 gctgcgcagc gtgcgctcga gatagacagc gcccgcgtag aacgggatgc caaacgtgac
      241 gacagaggcc tcggtcaccg ccgacgtata acactcaggg cacgcggcgc gagcaggacg
      301 gccacgacag tcacgcaaag ctccatccac tgcgaatagc ggacgtctgg tgccgccgcg
      361 aggaagaacc actgcagctg gtagccgatc gcgctgagca gcagtgacac ctcgagcacg
      421 cgcttcgtgc agaacggaag cagcacgaag ctcaacacga agtacggcag aatccagtag
      481 atcacggtca agctgatcca gatcgacgcc tcgatcagcc tggcctggat ccgactcggt
      541 ccggcgtcgt gctggagctc tgggatcgtc tccggcgcgg cgatcacgtt gaaccagacg
      601 tagacgctgg tccacgtgtt cgggcgatgg cgttggatca cgcgctggaa gttttcccag
      661 cgatacgcga ggtactcgac gggatgggcg agcacgacgc gtttccaggc gcgcaagagc
      721 gcggctcgtt gctcgtcgtt cgcggggatg tcgaacatgc gcccggcatc gcgcatcaag
      781 tggtagtgat ccaccgggct gtagatcgcg tgcattcgct gccagatgtt gtcgtgatag
      841 aggagcggaa cgccgtcgaa gatctcgtta agctcctcgt cgggccgcgg ccccagatgc
      901 tcgagcgtgc cgcagatgtc gagatacgcg tggccccagt accagagatg ctcgtgtgtg
      961 gtggtcaaca tctcgttcgt tccgtacgcg gcggctgtca cgccgaacca catggccaga
     1021 gcgatcgcgt aacgccgcca gccgcgcagc gtcggcgacc agcggaacag gagcagcatc
     1081 ggcgggaacg tcgcggcgag cgcattccaa cgcatcagac tcgcaaagaa cacgaacagt
     1141 agtgcgaggc gatgccggct cggccgctcg tcgagcatga ggccgatcgc gatcacgagc
     1201 gagccagcca tgaggccgtc cttcgcgatc aggccctggg cgccggagat cggcggccac
     1261 aggaacactc cagcagcgca caacgcggcg acacgcgggg agaaccggcg cttgaagatc
     1321 atgttcaagc cgacgagcag cgtgatcgct tgaacgagca gcacgccggc gggacccgtg
     1381 atgaagatct cgcagatgcg caccaacagc gggacggccg gtggatggcc gtcgctgaag
     1441 ctgaacgcgc gcgcttgcag gatgtgatcg cgggagtccc agccgatgaa gccgggccac
     1501 gcgtagacga ggaatgcaaa cagcgcggcg agcaggatca tgcgcggcgt gactcgtggg
     1561 tcgagccatc gccaggcctg cttcgcgcgc tccatcacgc tggcaatatc acagctgcgg
     1621 accggcgcgg ttggtaggct gccgccgcgc acggcgggcg atcagcatcg cgaccgccac
     1681 acacgtgcag acgaccgtcc agtgcgagta gcgatagtcc ggcgtcggcg cgagaaagaa
     1741 cagcgtcgac tcgacgacaa gcccactcgc gagcaccgcg aagacgtcac gctgaccgcg
     1801 cgagaacggc aacagcgcga gagcgagcac gatgtagagc cactgcgtgt agatcggcgt
     1861 gtgcgtccac agccagttgt atgcattcga ccatttgttc tgatacggac ggctacgctc
     1921 gggcaggcca aggttcgtga gaaagccttt ccaatgcatg atgcggggcg gaatcgcggt
     1981 acgcgtcttc accgtcacgc cgatcgcatc gaggaagatg acccagcgat ggtggagata
     2041 cgcgccggga tggccgaaca cgaggctctt ccacgcgcgc tcgatcgcgt cgcgctgcgc
     2101 ctgcggtgcg ggcacccggc cgttgatcgg caagtcccac agctgggtgt cgctgtagac
     2161 gagcttcgtc caatcgtgca gcgcatagac ttcacgaatg cgctgcaaga cgttcgtgtc
     2221 gatgagtagg ccggtgccgg cgagctcctg tttgacctgg tcttccgtga atccgtcgtc
     2281 gtagttgatg acgccggcga tgtcgtggat cgcgagcgag ctgtgccaga agtgcatctg
     2341 ctggtcggtg agtagcgagt tcgtacccat cgcggcaatc gtgacgccaa gccacgcggt
     2401 ggtcgcgagg gcgtagcgct tgagccacgg caggccttgc ttccactcga acagcagcac
     2461 gatcaatggc agggtcgcag cgaacgcgtt gtacttgacc gcggtgccga cgcagaagca
     2521 gacgagcgac gcgactcgcg cccaccggca tggcgacagc acgagcccgg cgccgagcat
     2581 caggagccca gccatgaccg agtccttcca catgaacgcc atcgggtttg cgaccggcgg
     2641 aaacaacatc agcacgcacg ccacgatcgc cgcgctgcgc tcggacatcg cgtgccgcag
     2701 gacgaggtac atgccagcga ggaacgtcgt cacctgcagc accagcagca ggaagccgcc
     2761 cggcatgatg ctgtcgagaa cccgccagag cgcagccatc gcaggcggat gacagtcggt
     2821 ataaaacccc tcgcgtgcct cggcgagctg ctcgaccgag tcgaacgtca tcaggcctgg
     2881 gcgcgcgaac gccaagaaca cgagcccgcc gaggaccaag atgatccttg ctcgggtgct
     2941 ctcgcgcatg cgcgcagact actacatcta ttagagacgg acgccggccc gcgtgggggc
     3001 gagacgccag agcattgcgg cgacggtgac gaccgcgatc gacatcgcga gagcagcacc
     3061 cgcaaggccg agcacgggta tcagcaggag attcgcgacg aagttcacgg caacgacgat
     3121 gagcacgaac acggtgtgcc accccgggcg attcgccatc agcaagactt gcgcgaacgg
     3181 caagtacggt gacgcgagcg ccaggcctcc catcaggatc gcgaacggta acgccccggc
     3241 ctggaacgag gcgtcgccga tcacccacgg gatgatgtac gggaacgccg ccgcaccgac
     3301 gaggcaggcg ccggcgagca ggggcacgaa ccagcgccgc gtgcgccgca cgagcgcttc
     3361 gacttcgtcg cggcggccag tggcgagcga ttgcgccatc accgggttga tgttgttcat
     3421 cacgaccacc gccagctgcg tcgcgccttc gttgagcgcg gcggcgaggc tatagatgcc
     3481 gaccgccgct ttcggaaagc cggctgcgcc gaccatccac acgtcgagct tggtctggat
     3541 ctcataggcg agcgtcgccg tgacgccacg cgcgccgtat gcgacatgct cgcgcgccca
     3601 gcgctgccag ccggcggcac gtgcgagcgt caccgtcgcg atcgtctcgc cgatcaggac
     3661 gagcagtagc gtgccctcgg tgaacgtcca gatcaccggc aggtgggcgg cgtcgatccg
     3721 catcacgtgc gctgcgacga tgccgactgc gatcagcacg tagcggagtg acgtgtagat
     3781 cgcgaacgcg cgcatccggc gttggccgtt cacgatcccg aacagcacct tgttgacggc
     3841 gaagcagaac aagccaggtg tggcgtacag catgccctcg gcgacttcgt cgctgccgtg
     3901 taggcgagcc accaaattgc gcgacgcgaa aaagatcgcg gtcgcgagcg cggcgagcac
     3961 gacattgggc acgagcgcac cgacaacgac ggccgccttg tgtgcgctat cgtcgggctt
     4021 ctcggcgact gcacgcagca cggagtactg gatgccccac gcgccgacga ctgcgagcac
     4081 gaagtacgcg atcgagacga gattgaaaat gccgagcgcg gccgcgtgcc accacgcggc
     4141 aatcaggatg ttcatcccga gcccgaccgc gccgagcagc acgaccggtg cgaggttcca
     4201 gatgacgtcg cgacggagcc gcttgtccat caatacagct tcggcgctgc gttgatgacc
     4261 tgcatcgcct cttcgaacga gaacccgatc agctgcgcgt actcgcgaag cgacgcccac
     4321 cgcggcttgc cgtattcctt tgcgcggcgc atcgcctcgt cgcgtgtcag gtggccttcg
     4381 cgcaccatgt tcgagagcat cgactcgtct tcggtgaaac cggcgatcgt cgcgtagatg
     4441 taattgtaga acgcggcggt gccgtcgccg atccgccacg tcgtggtcgt gtcaggcgag
     4501 acttcccagt cgtactcgcg gcggatcgtg ccgacgatct cgtcctcgtg ccacggcagg
     4561 tagtggtaga ggtacaggaa gtcgtccttg acgatgaacg tctgataaaa cgcgttcgcg
     4621 gtgtcgagga tcgactcgtt gatgtacgcc gggttcttcg cgtagttctt cgcgaagtac
     4681 cagagcatcg atgccttgtt ccgcacggac atgttcggca ggaccatgtt gtcgtcgtcc
     4741 tgcggcacgc ccatgaagcc gaccttgtag cgcgcttcct cgatcaggtt cccggtgcag
     4801 aagatgacga gcttgatgcc ggtctctttg cgcagctggc gcgcgtgcga gtagaactcc
     4861 ttgtcgccag ccatgaacag cgacaccatg ccgagctcgg gtttcttgag ccacgcctcg
     4921 atgttcttgc gcacgtagcg gcgcttcgcg gtgatgtcgg cggaccggat gatgtgctcg
     4981 atgccgagct tgccgcacac gcgggcgcag ttgcggcgcg cgaggtcggt gaccatgccc
     5041 cagtcataag tgaacgcgac cgggttcatg ccgagctgcg tcttgacgta gtggaggccg
     5101 taggacgagt cgcggccgcc cgagaacgcc acgatcacat ccgggctgcc gtccttgctg
     5161 cggtagggct cgacggcgcg gagcagctcc tcgacgccct tgggctggat cggcttccac
     5221 tgccggcagt acgcgcagac gccgtgctcg tcgaagccga cgaacggaaa cgtttcgggc
     5281 aggatgcact tcgtgcagcg acgcagccga tcggcgtgcg agctgtgatc gacgatcgag
     5341 acctggtggc cgttcggcgc actgccaaac ggcggctcgt cgcggtcggg ggccagggaa
     5401 aacgcacggc gttgcagcgt agcgagcgaa accgcgagcg catggccggc acgaatctgc
     5461 tcgagccgga cgtcgccgag ccggctgctg agatccttgt cctcgatcac acgctggagg
     5521 atgaagcgct ccgacgcgaa cgcgatcacg ttgccggcat cggtcgacag ctggaacagc
     5581 gacccggtgt tcgtcgcgag caacatgacg tcgaggttgt cgaacagcat cgcgatcgag
     5641 gctgcgccct cgagctccgc aaacgtcgcg cgggtggcgg caacgatgtc gccgctggcg
     5701 ttcagtttct tgcgcagcag cgtcgcgagc acttcgctgt cgagctcgct ctgcggcgcg
     5761 atcgcgtacc gctcggcaag cttgcgatcg ttgacgacga tgccgttatg gagcgcgacc
     5821 gctccgtgcg tgacaaccgg ctggttgttc gacatgtcgg cctgcgtgcc attcgtcgcg
     5881 agccgcgagt ggcccgtgat cgcgatcgta ttgccggccg cgaaccgagt gagcgcggtg
     5941 tcgagcagct gctggagcac cgggttcttc aagaagtcgc tgaccgagcc gccttgcttg
     6001 aggacctgga tccgttcgcc gtcatgcacc gcgatgccgg cagcctcgcg accgcgggtt
     6061 tcggaaaacc ggagcaagga ggtcgcgagc gcgcgcgcga ggtcgcgatc gaccttctga
     6121 tcgctcgcac aaatgacgcc gaagatgccg cacatgaagt ttcgagccga ccatacacgc
     6181 gtcggccaaa cttgtggata tctctcgcgg aaccacgtga tatagctcgc gcagctatcc
     6241 gtgcgcctcc tcctgataat tccggcgtac aacgagcaag aggcgctagg cggattgctc
     6301 ggcgagattc gcgcattgcc atcgctcggc gtcgatgtcg agaccgtcgt ggtcgacgac
     6361 ggttcagcgg atcggaccgc tgaggtcgcg cagcagggtg gtgcgcgcgt ggtccggctg
     6421 tgccgcaacc tcgggatcgg cggtgcggtg caatcgggcc tgcggctcgc gcatcgcgag
     6481 ggcttcgact tcgccgtgca gatcgacggt gatggtcagc acccgccgag cgagctcgcg
     6541 cgcctgctcg agccaacgcg aggtgcatcg ccgcccgatc tcatcgtcgg cacgcgctac
     6601 cgtgacgcgg cgaacttccg ctcgacgccg ctgcgccgac tcggcagctg gtggctgcgc
     6661 gtgatcctgc gcatgctcgc tcgcctgaag gtcagtgatc cgacgtctgg cttccgcctc
     6721 tacaacaagc gtgcgctccg gctgttcgac gagacatacc cgtacgattt cccggagccg
     6781 gaatcactcg caatcgcgcg cgctgcgggt ctcagagtct ccgaggtctc ggtaacgatg
     6841 cgcgaacgcc agggcggcac atcgagcatc attggcttcg attcgatcta ttacatgttc
     6901 aaggtgacga tcgcggtcgt gctcacgtac gtgcgtgcgc tgggccgcgc tcgcaagaag
     6961 ggatccgatg cagcaaccga ctgaggagta ctaccggttc catggcggtg ccggaaactg
     7021 ggtattcgcc ggcgtgtgcc tcgtgatcgc gatcgtgctc gcgcgcctca tcctgcgcga
     7081 gcggatctcg ttgcaaggca gcatgtcgta tctcgcgttc gtcctcgtga tcggcgcgat
     7141 ggcgttgttt cccgaacaag ccggtgcagt cgcccacgcg ctcggcttct cgacgctcgc
     7201 aaacttcttc ttctgcacct gcatcgcggg gctggcgatc ttgcacctcc gtgcactcgt
     7261 cacgctgtcg cgcgtgcact tgcgatcagt tgtcttgacg caggagctcg cactgctgca
     7321 agagaagctc gatcgcgcga tcgagaagcg atgactactg cgtagcttct aggtgataca
     7381 ggccaccgag ctgctgcgtg ccgcagcatt cggtcgtggt caggtagagc tccccacgcg
     7441 aatcggcgtg caggcttgcc ggcgtgctgg gaaagcctgc gcgcggctgg gccggcggct
     7501 cgacgaacgt cgtctccggg aactcgacga cgacgttgcc agcgcccagc tttgcggcgc
     7561 cgagcacgtg cgcgcagtag tcggtgaaga aatacgtgcc gacgaggtcg ggatagcagc
     7621 tgccgcgata gaccgcgccg ccgatgaccg agcaccagcc gtcgctgtgc atcttctcga
     7681 actgcggcat gcgaatgctc gcgggattgc acgggccgtc gtagcagcga tcggcctcgt
     7741 acatgcgcca gccgagattc gcgcctgcct gctcggcggc cgggatgaag tcgagctctt
     7801 cgacctcgtc ctgaccgacg tcgccgatcc acatgtcgcc ggtcgtcgaa tcgaacgacc
     7861 accgccacgg gttgcgcagg cccatcatgt agacctcggg agcgccgccg gtcgtcgcga
     7921 acggattcgt cgctggaatg ccgtactcct tgcccggcgc tttgttgtcg acgtcgatgc
     7981 gaagcatctt gccgagcagc gcgttcgggt tctggccgtt cgcctgcgga tcgccgccgc
     8041 cgccgccgtc gccggtgccg atgtagagat aaccgtcggg gccgaactcc atcatgccgc
     8101 cgttgtgatt cgacgcgaag tcggggatcg acagcacgat cgttccgctc gccttgttcg
     8161 cgacgttggg atcgctcgac acctgatagc gcgcgacgac gttcgcgttg cccgtcgtgt
     8221 agaagacgaa gaacgtgccg ttcgtcgcgt actgcggatg aaatacgagc ccgagcagac
     8281 cctgctcgcc gcaacacgcg atgtcgtcgc cgagatcgag gaacggtgtt gcgagcagcg
     8341 tgtcgttggt gaggacgcgg atcgtaccga tttgctcgac gacgaacagt cgcgggtcgg
     8401 ctggtggaga tgtcgcgagt agcgcagcgc cgtgtgtggt cgcgatgagc cgcatcgaga
     8461 tcttgctgcc ggactgcggc gagcaagcga gcgcgggtgc atcagagacg agcccactat
     8521 cgagcttcgt cggcgctttg tctccgcacg cgatcagcgc gagcgcgagc gcggctcgtt
     8581 tcatcacatg cccgcttcga ggtggaagat gccgccctgc gtttgcgagc cgctgacttg
     8641 ctcggtggtg aggtagagct cgccgcgcgc atcggcgtgg aggctcgacg gtgtcggcgg
     8701 cacgccgtcg tggatgccct gcgcgtcggc gtagtgcgtg gtcggtttct cttcggcgag
     8761 cgcggtgccg ttctgcttgg ccgcgaccag ctcgtgcgcg cagtagtcgg tcacgtagcc
     8821 ggtaccgacg atgtcgggat aacagctgcc gcggtagacc tgaccggcga tcacggcgca
     8881 ccacgcatcg ccgctgtgcg tcttctggat cgcgggcatc gccatgcctg ctggatcgca
     8941 cggctgcgag tagcagttcg cgccctcgta catgctccag ccgaggttca tgccagcggc
     9001 tttgccgttc gggatcacgt cgagctcttc gaccgcgtcc tggccgacat cgccgatcca
     9061 cagatcgccc gtcgtgcgat cgaatgacca gcgccacgga ttgcgcacgc ccatgacgag
     9121 gacctcgggc gcaccgccgc ccttcgcgta tgggttgccg ctcgggatgc cgtactcctt
     9181 gccgctttcc ttcttgtcga catcgatgcg gaggatcttg ccgagcagtg cgtttggatt
     9241 ctggcccgtc ttcatcggat cgccgccgcc cccgccgtcg cccgtaccga tgtacaggta
     9301 gccgtccttg ccaaactcca tcatgccgcc gttgtgattg ccggcgaagt cagggatcga
     9361 aaggatgacc tttgcgctcg ctggatccgc gacctcggga ttggtcttgc tgacggtgta
     9421 gcgcgcgacg acgttcgcgt cgccggtcgt gtagaagacg aagaacgtac cgttcgatgc
     9481 gtacttcgga tggaacgcga ggccgagtag accttgctcg ccgcccgccg cgatgaggtt
     9541 cgacacgtcg aggaacagcg tcggcgagat accagcgtcg gtgagctgct cgatgatgcc
     9601 gtcgcgctcg accacgaacc gccggatgtc gtttggcggt gatgtcacga gcagcgccgc
     9661 gctcttcgtc cgtgcgacct cacggaacgt gatgctcgtt ccgtggaccg gtgcttcgca
     9721 ggcgggtggc agcgagggcg tctcatcgcc gccacagccg agaagtgcca acgccagtgc
     9781 tacgtacctc atttactaag gataagacga tcacggagtc ggtggctgtg agaacggcca
     9841 ttgcgccccc ggctgctgag catgataaga ggagacaatg gcttcgccga cgttgtcgat
     9901 cgtggttccc gcctataacg aggagcgcaa tatcccagcg tttctcgcgg cgatgatccc
     9961 ggtcctcgaa ggcattggcg aaccgttcga aatcattttc gtcaacgacg gcagcaagga
    10021 cggcacgctc ggaatgctcg cggccgcgtc gtcgcaagat ccgcggatca aggtcgtggg
    10081 ccttgcacgc aacttcggca aggacatcgc gctgtcggcc ggcctcgcac acgcgaccgg
    10141 caaggccgtg atcccgatcg actgcgatct gcagcatcca cccgagctcg tcccgcagtt
    10201 cgtcgcgaag tggcgcgaag gcttcgacat ggtgatcggc gtgcgcagca agcgcgacga
    10261 agaaggctgg ctgcgccgca cgctgtcgca gacctactac aaggtcatgc gttcgatgac
    10321 gagcgtcgag attccgccga acgccggcga cttccggcta ctcgatcgca agatcctcga
    10381 cgtcatcaac aagatgccgg agcgccaccg cttcatgaaa ggcatcttcg cgtggcccgg
    10441 cttcaaggtc gcgtcgatcg agttccaggc gaacgtgcgc gcgaacgata cgcgctcgag
    10501 ctggtcgttc ttcaagctgt ggcgattcgc gctcgacggt ctgttctcgt tctcgaccgc
    10561 gccgctcaag ctgtggacgt acatcggcct gctctcggca ggcggcgcat tcatctacct
    10621 cacgatcacg ctgatccaga agttcttctt cggcatcagc gcacccggct acgcctcgct
    10681 gctcgtcgtg ttgctgttct tcaatggcct cttgatgatc agcaacggca tccaaggcga
    10741 gtacatcgcg cgcatcttcg aagaagtgaa aggccggccg ctgtacgtcg tcggtcagac
    10801 gttcggattc gatgcgcagc agccggtcgt cgagaagccg gaggtcgtcg tcacgatcgc
    10861 cgagcccgtg ccggtcgcgc cgcccaagcg cgagaagccg ccgctgccga agccgccatc
    10921 gctgccgcgc ccgcagtgat cactgcccga gcgcgaagtc gatcgagaag ctcgggttcg
    10981 acacgatgcg gttgttgacg acgtcgagga tcgtcgccga gcactcggtt gccgtgaacg
    11041 acgtcatctc tggataacca tcggcgttgc gcacctgcgc cgacgccggg atgccgaagc
    11101 agccgaacgc gagattgagc acgtcgatct cggagtggag caggatgctc gacttgcctg
    11161 tcatgcacag ctgcaggcgc acgtcccagt acgcgagcat gtcgagaatc tgggtctgca
    11221 ccaattgcgc gctcgcgccc gaggcggtgc gctcggcggg gaacgtccat agcatcgtgc
    11281 tcttgcagtc ggtcgtgtcg ccctcgtagc cgcggtcgac ggcgaaatcg ggaaacacga
    11341 tctgcatctc gatcgtctgc atcgcgagtg gatcgagcgg cgacattcct gcgccggtca
    11401 cgctggtgac gacgaagctc gtggtcgcgg tgagccccgg gccgccgccg ccgccgtcgc
    11461 cgccgcatgc agcgagcagc gcgagccacg agtgcttcat gcggcgagct tagcttgcga
    11521 caccgcagac gcgcgtgata tcgcccacgt gatgaagcgg gccgcgctca tctggctcgc
    11581 gatcatcgtg ccgatctggc tcgtgctcgc gctgtgcacg tactgggagc cgatcatccg
    11641 cgacggctgg ggctttctgt tctttcaccg caagtacgga ctgtcgtgga cgaccgccga
    11701 ggcgttcatg aagaacaact acaacgcgaa caacccgcgg ctcgggcagc tctacacgct
    11761 cgtgatgtac acgcccgggc cctggcacgt gatcgtcacc ccgatcgtcg agctcggcct
    11821 gttctacgtg ctcgcgacgc tcgcgcttgg tcgttggcca tcgctgcgcc gcaccgacga
    11881 cgcgctggtg ttcgtgacga tcgtcgcgat ggtgttcgcg accgcgcgct cgcttggccc
    11941 actgttgttc taccggccgt tcatgggcaa ctacacggtc gggctgctgc tgaatctgct
    12001 gtggctggtg ccgtatcgct tgcacgccga gacgcgctgc atgcgctggt ggtggacacc
    12061 cgtgatgctc gggtttggcg tcgtgagcgg gctgtgtaac gagcacaccg gtcccgcgct
    12121 gctcgccgca ggcttgttcg cgctggtcgt ctactggcgg cgcggcgagc gcatcgtgcc
    12181 gtgcgcctgg gccggcgtca tcggcatggc gatcggcatg gtcattctgc tcggcgcgcc
    12241 cggacagcac gttcgctacg ccggcgctgc agcacgcgaa tcgatcatcg acgcggtcat
    12301 ccagcgcggc gtcggcggtg cggcgtgggt cgtgcttgcg ctgccgttgt tcctgacccc
    12361 gctgctgttg tggctcgtgc cggtcttcgt cgcgcggcgg cgcggatcgc gcagcgaaca
    12421 gccgcgctcg cagatcgttg ccgccctcgt cgcactcgcg atggcgacgc tgatggtgtt
    12481 cacgctgctc ggctcgccgc gttggggcga tcggatgtac ttcgcatcga tctgtcttgc
    12541 ctgcgccgcc gcggccagct gggtctgcgc gcatcccggc acactcgcgc gccggagcac
    12601 gctcactgtg aacatcgcag cgctcgtcgt cgtgagcgcg atcttgctgt tcatgcatca
    12661 ccgcctgcac gacgagttca ccgcgcgctt cgcaatcctc gagcatgcgg cgcccggcac
    12721 cgtcgtgcgg gttccgacct actcgcagga gcgctcgcgt tacgtgctcg gcgacgactt
    12781 cctcgttccg acgacacgcg gcagcgtcgc gaaggcgtat cagttacagg acatcgagct
    12841 cgatcaacgc gtgccgacga cagcgccgtc ggacgagccg tagattgcat acagaccctt
    12901 cgtctgggtc tcggtgttga cgcggatccg atcgatccgt ttgtcggtct tcaggtcgac
    12961 aacggtgaac ttgccgtcgg gcaaaatctt atcgatccgc agtcgcttcg tcgtgccgtc
    13021 gtcgaagtag accttcacgt cgcgcaggat cgtcttgccc tgggacgaga accgcagctt
    13081 cgagaacgac cccgcctctt taccgaccat cacgatttcc gtgccgtgct tcgcgggcgt
    13141 cggcgacgcg agctcgatcc actcaccggc ctcacgcggc tcgggtttct tgttgccctt
    13201 ttcgtcgagg cgcacgcgcc cgttgttctc ctcttgttgc ttctgctgcg cctcttgcgt
    13261 gggcggcacc ggtttgggcg ggtccgcata actcacggag ctgccgacga tgatcgcgaa
    13321 aagtactgaa gacctcatag caaatcggcg aggttgcata ggccgtgccc caggttgata
    13381 gtccgtagat ggagatcccg ctcgagctca tccaacgcct gccgaagacc gacctgcact
    13441 gtcacctcga tggctcgctg cgtctcgata ccgtgctcga cctcgcgcgg aagcagaaca
    13501 tcaagctgcc tacgttcgat cggtccgagc tgttcaacat gctctacgcg ggcgagcacg
    13561 tagcgtcgct cgacgactat ctgcgcgcct tcgacatcac gctctccgtg atgcagaccg
    13621 aagaaggcct cgagcgcacc gcgtacgagc tcgcgtgtga cgcgtgggcc gagaacgtgc
    13681 gctacatcga agtgcgctac tcgccgctct tgcacacgcg cagcggattg cgcccggcgc
    13741 aggtggtcga agccgtactg cgcgggctac gtacggccaa gcgcgagctc ggcatccgct
    13801 acggcgtcat cctgtgcgcg attcgcacgc tgagccccga gtcgtcgctc cgcatcgcgg
    13861 agctgtgcgt cgcgttcaag aaccgtggcg tggtcggctt cgatctcgcg ggcagcgagg
    13921 tgaacaatcc ggcgaaggtg catcgccagg cgttccagct cgtcatcgac aacaacatca
    13981 actgcaccgc gcacgcgggc gagtcgttcg gtccggactc tgtacatcag gcgatccaca
    14041 agtgcggtgc gcatcgcatc ggtcacggca cgcgcctggt cgagagcggc gacttgctca
    14101 actacgtcaa cgaccatcgc atcccgatcg aagtgtgccc gtcgtccaac gtgcagacgc
    14161 gcgccgcgaa cagctgggag actcacccgg tcgacttctt tgtcgactac ggcgtgcgcg
    14221 tcacgatcaa caccgacaac cggctgatgt ccgacacgac ctgcagtaaa gagctctacc
    14281 tctgccacaa gcactacggg tggtcgctgc agacgatcaa agagatcgtt atttcgggct
    14341 tcaagagcgc gttcatgccc taccgcgaga aagcggacct ggtcgccgag gtcagccgcg
    14401 agctggcgaa gtacgaagac ccgcggggcg aggtgacagg ccgcgtcggc ctcgtggagt
    14461 ccggacagcc gacttcatcc gacaaggcga ccccgccgac cccgcccacc ccgccgaagg
    14521 cgaagctcca agtgtcgtaa accgctacgg ggcggtcatg aagtcgaacg acgtcgggac
    14581 gaacgcgttg ttcgccggca cgtcgaagat gcccgcatcg acgtagaccg tgatcgtcgc
    14641 gttcgcgggt agcgccgatg ccggcatgaa cgcgtatttg aacggatcga tccccatgac
    14701 cgtgccgggg atcgagacgc tgctcgactc gaggcgaaac gtggtgttgc tgacaccaga
    14761 tacaggctcg ttgaattgga tcgtgaccac ttgcgaggtc ggcacgttcg aagcgccgct
    14821 caccggatcg gtcatgaaga tctgtggggg cgtgatgtcg gcgtcgggtg gcggcgcgtc
    14881 gatcgggcgt ggcgagtcga tacgcgcgtc gattgcgcac accgtcgtgg ccggcgtccc
    14941 gttgcggtga cagatggcgt cagggccgca aaagtaatcc gacgggcact cgccgctgcg
    15001 acggcaggca aaaccgcagt ccggttgcgg cggcgaatag cacgccgtta tcaggcccag
    15061 gcaaatcgcc aggatccggc cgagcacggc gagcacggta tcacgtcaca ctgcgagaaa
    15121 tgcgggctgg gtgatccaca cggagaagtc agcgtagtga aagaagcccg ccggtcgatg
    15181 ctggtgtacc atgtggcccc tgatggccaa gctggaacgt gaacagctgc gcgggctcgt
    15241 cgagcctcac tggcgcaggc tgtacaactt cgtgtttcgg ctcacgctcg accgcgatcg
    15301 cgcggagcgg tacctcgccg acgtctttct cgcggccacc aacaagttcg attcgaagcc
    15361 cgcgaaagac gatgagatcg aagtctggct gctcggtatc gcgaaccaga tgctcgaaga
    15421 gcgcctgccg cgtcagccgg aagtcaactt cgacattctc gatgagacgc tgcgcagcga
    15481 gtcgacgcgc accgatgtcg tgcgctcgct gagcgatccg cagcgcgact tcctgctgtg
    15541 ggagctcaag cagggttgca tgaccgcggt cgtcaactgc ttgccgcccg gcgagcgcgc
    15601 agcgttcgtc gtctgccacg tgctcaagct gggcgacgac atcgcggcga agagcctcgg
    15661 catcaccgag agcgcgtaca aggttcgtct gtcgcgtgcg cgcaagaagg tgggcgacta
    15721 cctcgcgccg cgttgcgagc acgtcaaccc gatgaatccg tgccgctgcc cggcgcgcgt
    15781 cgggacggcg ctcgcgaagg gcttcatccg cagcgtcggc ccggtcggcg gcgaagtctc
    15841 gctgcgcaag ccgagcgagc cgtacgggcg ctatggcact ggcatcggca atgaggacgt
    15901 accgatgcgc gacatcagcg cgatctatgg cggcctgccg gatccggatc cgccgggcga
    15961 cctcggcaac aagctcatcg accagctgtc gcagtagcgc ggtatgctgc ttcggtgagc
    16021 tcgcgcgata ccacaccgga tgcagtcgcc gtacgaacgg cggtgtaccg ccggatgtca
    16081 gacgagcagc gcgtgagcat cggcgcgcag atgtcggagg acgcccgcgc gatcgccctc
    16141 gatgcgatcc ggcagcgcca tccagactac gacgaagagc aagcgacgtt cgcgctcttt
    16201 cggttgctgc tcggtgatga cttgtttcgc cgcgtttggc cggctgcccc actgcttcgg
    16261 ccatgactcg cgatggtttg cagctactac ttcaggacgt agttggcctg ctgtcggcga
    16321 tcggcatccc tcacatggtc gtcggatcgt tcgccagtat ggtgcacggc gagccacgaa
    16381 ccacgcagga cctagacgtc gtgatcgacc cgacggccga gcagctagag caactgctcc
    16441 agcggctgcc tgcggatcgg tactacgtcg atccggatgt tgcccgcgat gcgcgccgcc
    16501 gcaggtcgat gttcaacgtc atcgacatgg cgtcgggctg gaagatggac ctgatcgtgc
    16561 gcaaggcgcg tccgttttct gtcgaagagc tgcagcggcg cgcaaaggcc acaatgcaag
    16621 gtgtcgtggt cgacaccgca accgctgagg acacgattat cgccaagctc gagtggagca
    16681 aggcaggcag ctccgaacgc cagctcgagg acatagcggg gatccttcgc gtccgtggcg
    16741 acaagctcga tcgcgcatac atcgagcgct gggtcgacga gctcgagctc cgcgagcagt
    16801 gggaccgcgc gcaacgcctc tgagctattg cttcgtccag ccgctgccgg tccagtgata
    16861 ggtctcggtg tactgctcgt cgtcgttcgg cgcgcgctgt agcgtgatct tgccgggctc
    16921 gaacgtgtac ttgtcgtcga acttgtcgtt gcggttgacg tcgaccttcg cgcgattgac
    16981 gttcgtctgc ccc
//
LOCUS       DASUFX010000306         5615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1435113, whole genome shotgun sequence.
ACCESSION   DASUFX010000306 DASUFX010000000
VERSION     DASUFX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435113"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..1262
                     /gene="uppP"
                     /locus_tag="VFV99_12055"
     CDS             447..1262
                     /gene="uppP"
                     /locus_tag="VFV99_12055"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX5060090.1"
                     /translation="MALWFAAVLGLIQGLTEFIPVSSTAHLRVAPALFGQPDPGAPFT
                     AVIQLGTLLAVIAYFAKDLAALPAAMFRDSGSFEGRLPWLLAVGTLPIVILGLTFKKH
                     IEGDLRSLYVVATALVVVGVAMAFIDHYAAGRPNHRPLSSLTLTDALLVGCAQSLALI
                     PGVSRSGATMCMILLLGLTRADSARFSFLLSIPAIAGAGIFEARTAFSQLGSSAIPAI
                     AVGTAVAAVTGYASIAWLLRWLGSHSLIGFALYRIACGGALLAALAAGFFRRV"
     gene            1317..3497
                     /locus_tag="VFV99_12060"
     CDS             1317..3497
                     /locus_tag="VFV99_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5060091.1"
                     /translation="MRISSIAFCAFAVVVLFVSTAGAAPCPLTASGTKYPIKIETSPP
                     GATIYFNDKTCSAGTTPWSGKITAGEVTINAELAGYDLGTRTVKVIRSRKTQEFFLPL
                     TKKAEPPKIEVKADADPKGVAGAQVWLDGEMKGQAPVTVTTTEGRHLLQLKKDGFETY
                     ETWISATTNNTSTLLPQMKEVAKPKFGTIVVEADVADAEVYIDGNKHPDNTPAMISNV
                     IEGLHVIEVRKAPSLPWKQTVQVKASETTKVRADLQSTMHGGVGVVRVLSDAPGARAF
                     LDGTDMGPVPVDIKDVKAGDHIVQVKAPSMQTGEKTVKVAAGSSQIVKFDLNSEAPAD
                     SGLLKVVSMVPEAQVFIDGAAVGKVPVEKRLSQGEHPVVVRLDGYKQFEQKVRVEAGQ
                     TVTVQASLKPVGRLRILSTPAQAQVVVNGLPIGKTPLDQEVEVGETVVRIEMAGFQPF
                     EQTLTIEGGKTQTLSRELAIAGLSESELASQQRGLSSFGARTLPRGRSTVDLMVGYPY
                     YVEAKINVGAGKIAKKFGFDAGVGVRSFLARNELGIGGRAMLADQDPFSAGIFTDLWW
                     GSRLLDDSRRNGLTWNVGAAASLTALSHVTITGRLYANIWSDRHCPEEKTTSSTGFDG
                     DPIQICVDYKNNTLMADERARINKLTTGSETETWDFSSRESGIRLMASVIAEIAFEQR
                     WNIFGILEGAPFQEERALFTNDFSGTMPETDYNLYLQLGLTYKF"
     gene            complement(3505..4059)
                     /locus_tag="VFV99_12065"
     CDS             complement(3505..4059)
                     /locus_tag="VFV99_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5060092.1"
                     /translation="MSGEHDHLADNIKALRETRGLSQQQIAKAAGVPRATWTHLESGG
                     ANPTLTVLIKVANALQVRLDELLAPPRQPARFLKADELPMRTKGQVAIRKLLPEPLPG
                     LDLERMVLPPGARMAGVPHTPGTREYLTCERGTVELAVAGEKYTLLEGDVVSFRGDQR
                     HAYHNPGSSTSVAYSAIAFAPVAG"
     gene            4145..4873
                     /locus_tag="VFV99_12070"
     CDS             4145..4873
                     /locus_tag="VFV99_12070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5060093.1"
                     /translation="MKIQGTGALVTGASRGLGRALAEALAAKGARVAMVARDAGPLAD
                     AVAAIRARGGIAHAIAGDISDKDAVHKIAGQAQGLVGEIGIAIHNASSLGPVPLRLLL
                     DTECEDLAAVLETNLVGPFRLTKILAGAMALRGDGTIVHISSDAAVEAYPRWGAYGVS
                     KAAQDQLSRVLAAELDGTGVRVLAIDPGEMDTKMHADAIPNADPASLQRPADVAARII
                     DIIENATAAPSGARLSVPSWKAAS"
     gene            4870..>5615
                     /locus_tag="VFV99_12075"
     CDS             4870..>5615
                     /locus_tag="VFV99_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5060094.1"
                     /translation="MMARAYRRYASLAPVEEARAVGGATLALRTDEVSRHAVRLVVIG
                     RNEDQVAITKFANLASLLRAGDLVVVNDAATLPASLFGTTERGDAFELRLSAPVDGNR
                     LYGVLLGRGDHRTRTEHRAPPPPIAVGDIVSIGALHAKVVAAAGRRVELIARESGDAL
                     WQALYASGKPVQYAHRPELLPLYAVQTAYASRPWAAEMPSAGRPLTWDVLLAMRRAGV
                     QIATLTHAAGLSSTGDDALDRALPWPERYE"
BASE COUNT          918 a         1812 c         1906 g          979 t
ORIGIN      
        1 cgagctgctc gatctcgcct gaaaggctca ggcctcgggc ctcgggcctc agcggggcat
       61 cagacttcag gctagccgcc gcatgcatcg agctcggcga gcgtatgcgc cccaagtgcg
      121 cactcgtacg tgtgcgccgt catcgcgttg cacttcggct cccacgcgat ccggagttgc
      181 ccctgaatgg tggtgcgctc ttcgtctgtg ggcttctggt cgtctgggcg ttctgcaatg
      241 cctagctcga cagtgtgtga gatcagcttg tcgcaatcgc gactcggcgt ttgattcgtc
      301 tgactgtggg attggtgctc gttgttgtga gccggaggtc tggggcctga gggctgaggc
      361 ccgtgaggct ttccaccaca cgccatcgcg agcagcacca cgcaaaggag cctcgtcacg
      421 actttgatgt tagcgtgtga ttcgcgatgg cgctgtggtt cgctgctgtg ctgggtctga
      481 tccagggcct caccgaattt attccagtct cgtcgacggc ccatcttcgc gtcgcgccag
      541 cgctgttcgg tcagcccgac ccaggtgcgc cgttcaccgc ggtgatccag ctcggcacgc
      601 tgctcgcggt catcgcgtac ttcgcgaagg acctcgccgc tctgccggcc gcgatgtttc
      661 gcgatagcgg aagcttcgag ggccggctgc cctggctact cgcggtcggc acgctgccga
      721 tcgtgatcct ggggctcacg ttcaagaaac acatcgaagg tgatctgcgg tcgctctatg
      781 tcgtcgcgac cgcgctcgtc gtggtcggcg tcgcgatggc gttcatcgat cactatgcgg
      841 ccggccggcc gaaccatcgg ccgctgtcgt cgctcacgct gaccgacgcg ctgctggtcg
      901 gttgcgcgca gtcgctcgcg ctgatccccg gcgtgtcgcg gtcgggggcg accatgtgca
      961 tgatccttct gctcggactg acgcgagcgg attccgcgag attttcgttc ttgctgtcga
     1021 tccctgcgat cgcgggggcc ggcatcttcg aagcaaggac ggcgttttcg cagctcggtt
     1081 cgagcgcaat ccctgcgatc gcggtcggta ccgccgtcgc tgccgtcacc ggctacgcgt
     1141 ccatcgcatg gctattgagg tggctggggt cgcacagcct gatcggtttc gctctctacc
     1201 ggatcgcgtg cggcggcgca cttctcgctg ccctagctgc aggtttcttc cggcgggttt
     1261 gagaaacggc cgcacggctg ttagcctcgt aggcgtccgt acttacgagg gtgtccatga
     1321 ggattagctc gatcgctttc tgcgcgttcg cagtcgtcgt gttgttcgtg tcgaccgctg
     1381 gcgcggctcc gtgcccactc acggcatcgg ggaccaagta cccgatcaag atcgagacgt
     1441 cgccacctgg cgccacgatc tacttcaatg acaagacctg ctcggccggc acgacgccgt
     1501 ggtcgggcaa gatcacggcc ggcgaggtca cgatcaatgc cgagctcgcc ggctatgacc
     1561 tcggcacccg aacggtcaag gtcatccgct cgcgcaagac gcaggagttc ttcctgccgc
     1621 tgacaaagaa ggccgagccg ccgaagatcg aggtcaaggc agacgccgat ccgaagggcg
     1681 tcgcgggcgc gcaggtctgg ctcgacggcg agatgaaagg ccaggcgccg gtcacggtca
     1741 cgacgaccga gggccgccac ctcttgcagc tcaagaagga tggcttcgag acctacgaga
     1801 cctggatcag cgcgacgacg aacaacacgt cgacgctgct gccgcagatg aaggaagtcg
     1861 cgaagccgaa gttcggcacg atcgtcgtcg aggccgatgt tgccgacgcc gaggtgtata
     1921 tcgacggcaa caagcacccc gacaacacgc cggcgatgat cagcaacgtc atcgaaggcc
     1981 tgcacgtgat cgaggtgcgc aaggcgccga gcctgccgtg gaagcagacc gtgcaagtga
     2041 aggcgagcga gaccacgaag gtgcgcgccg acctgcagtc gacgatgcac ggcggtgtcg
     2101 gcgtcgtccg cgtgctctcg gatgcacctg gcgcgcgcgc gttcctcgac ggtacggaca
     2161 tgggcccggt gcccgtcgac atcaaggacg tcaaagccgg cgatcacatc gtgcaggtca
     2221 aggctcccag catgcagact ggcgagaaga ccgtcaaagt cgccgccggc agctcgcaga
     2281 tcgtgaagtt cgatctcaac agcgaagcgc ccgccgatag cggccttctg aaagtcgtgt
     2341 cgatggtgcc cgaggcccag gtgttcatcg acggtgcggc ggtcggcaag gtgccggtcg
     2401 agaagcgcct gtcgcaaggc gagcacccgg tggtcgtgcg cctcgacggc tacaagcagt
     2461 tcgagcagaa ggtccgcgtc gaagcgggcc agaccgtgac ggtgcaggcc tcgctcaagc
     2521 cggtcggtcg cctccgcatc ctctcgacgc cggcgcaggc gcaggtcgtc gtcaacggac
     2581 tgccgatcgg caagacgcca ctcgatcaag aggtcgaggt cggcgagacc gtcgtgcgta
     2641 tcgaaatggc cggcttccag ccgttcgagc agacgctgac gatcgaaggc ggcaagaccc
     2701 agacgctgtc gcgcgagctc gcgatcgcgg gcctgtcgga atcggagctc gcgtcgcagc
     2761 aacgcggcct gtcgtcgttc ggcgcgcgca cgctgccgcg cggccgctcg acggtcgatc
     2821 tcatggtcgg ctatccctac tacgtcgagg cgaagatcaa cgtcggcgcg ggcaagatcg
     2881 ccaagaagtt tggcttcgat gccggcgtcg gcgtgcgctc gttcctcgcg cgtaacgagc
     2941 tcggtattgg cggtcgcgcg atgctcgccg atcaggatcc gttctcggcc ggcatcttta
     3001 cggacctgtg gtggggcagc cggctcctcg acgattcgcg ccgcaacggc ctcacgtgga
     3061 acgtcggcgc tgcggcgtcg ctgaccgcgc tgtcgcacgt cacgatcacc ggccggctct
     3121 acgcgaacat ctggagcgat cgtcactgtc ccgaggagaa aacgacgtcg agcaccggct
     3181 tcgatggcga tccgatccag atctgcgtgg attacaagaa caacaccctg atggccgacg
     3241 agcgcgcgcg gatcaacaag ctgaccaccg gcagcgagac cgaaacgtgg gatttctcct
     3301 cgcgcgagag tggcatccgc ctgatggcga gcgtgatcgc cgagatcgcg ttcgagcagc
     3361 gctggaacat cttcggcatt ctcgagggtg cgccgttcca ggaggagcgc gcgctgttca
     3421 cgaacgactt ctcgggcacg atgccggaga ccgactacaa cctgtacctg cagctcggtc
     3481 tgacgtacaa gttctgagcg tcggctagcc ggcgaccggc gcgaacgcga tcgccgaata
     3541 cgcgaccgac gtcgagcttc cagggttgtg atacgcgtgc cgttgatcgc cgcggaagct
     3601 gacgacgtca ccttcgagta gcgtgtactt ctcgccggcg accgcgagct cgacggtccc
     3661 gcgttcgcac gtcaggtatt cgcgcgtacc cggcgtatgc ggcacgcctg ccatgcgcgc
     3721 gcccggcggc aacaccatgc gctcgagatc gagaccgggc agcggttcgg gcaacagctt
     3781 gcggatcgcg acctgaccct tcgttcgcat cggcagctcg tcggccttga ggaagcgtgc
     3841 gggctggcgc ggtggtgcaa ggagctcgtc gagccgcacc tgcagcgcgt tcgcgacctt
     3901 gatcagcacc gtcagcgtcg ggttcgcacc gccggattcg agatgcgtcc acgtggcgcg
     3961 cggaaccccc gccgctttcg cgatctgctg ctgcgacaag ccgcgggttt cgcgtaacgc
     4021 cttgatgttg tctgcgaggt gatcgtgttc tccgctcaac ggctgacctc ctgccaatag
     4081 attagcgcga ccggtgacag attggcggtg tgtgggtacc cctaattgtc aggaggcatc
     4141 gaccatgaag attcaaggaa caggcgccct ggtcaccggc gcgagccgtg gcctcggacg
     4201 agcgctcgcg gaggcgctcg cggccaaggg tgcgcgggtg gcgatggtgg cgcgtgacgc
     4261 tggcccgcta gctgacgcgg tcgcagcgat ccgcgcgcgg ggcgggatcg cgcatgcgat
     4321 cgcaggcgac atcagtgaca aggacgcggt gcacaagatc gcgggccagg cacagggcct
     4381 ggtcggcgag attggcatcg cgatacacaa cgcgagctcg ctcggcccgg tgccgctgcg
     4441 gctcttgctc gacaccgagt gcgaggacct cgctgcagtg ctcgagacga acctcgtcgg
     4501 accgttccgg ctgacgaaga tcctcgcggg cgcgatggcg ctacgcggtg acggcacgat
     4561 cgtgcacatc agctcggacg cggctgtcga ggcgtacccg cgctggggcg cgtacggcgt
     4621 gtcgaaggca gcgcaggatc agctcagccg cgtgcttgcc gccgagctcg atggcaccgg
     4681 cgtgcgcgtg ctcgcgatcg atcctggaga gatggacacc aagatgcacg cggacgcgat
     4741 tcccaatgcg gacccagcgt cgttgcagcg ccccgcggat gtcgccgcac gcatcatcga
     4801 catcatcgag aacgcgacag cggctccaag tggcgctcgg ctcagcgtgc cgagctggaa
     4861 ggcggcgtca tgatggcgcg tgcgtatcgt cgctacgcca gtctcgcgcc tgtcgaagag
     4921 gcgcgcgcag tcggcggcgc gacgcttgcg ttgcgtacgg acgaagtctc gcggcatgcc
     4981 gttcggctgg tcgtcatcgg tcgcaacgag gatcaagtcg cgatcacgaa gttcgcgaac
     5041 ctggcgtcgc tgctgcgtgc cggcgatctc gtcgtcgtca acgacgcggc cacgttgccg
     5101 gcatcgctgt tcggcacgac cgaacgcggt gatgcgttcg agctacggct atcggcgcca
     5161 gtcgacggca atcggctcta cggcgtgttg ctcggccgcg gcgatcaccg cacgcgcacc
     5221 gagcatcgcg cgccgccacc tccgatcgca gtcggcgaca tcgtcagcat cggcgccttg
     5281 cacgcgaagg tggtcgctgc cgcaggccgc cgcgtcgagc tgatcgcgcg cgagtcgggc
     5341 gatgcgctgt ggcaagcgct ctacgcgagc ggcaagccgg tgcagtacgc acaccgtccc
     5401 gagttgctgc cgctctatgc agtgcagacc gcctatgcga gcaggccgtg ggcggccgag
     5461 atgccgtcgg cgggccgccc gctgacgtgg gacgtgttgc tcgcgatgag gcgcgctggc
     5521 gtccagatcg cgacgctgac ccacgccgcc ggcctatcgt cgacgggcga tgacgcgctc
     5581 gatcgggcgc tgccctggcc cgagcgctac gagat
//
LOCUS       DASUFX010000307         9410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1992566, whole genome shotgun sequence.
ACCESSION   DASUFX010000307 DASUFX010000000
VERSION     DASUFX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992566"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFV99_12080"
     CDS             <1..1035
                     /locus_tag="VFV99_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5060095.1"
                     /translation="IATPLSGWWIDRIGARRGLVASVLLWTAVAALHALIPGFWVLFA
                     LRIALGIAEGPSFPGAAQTVQRILPPADRARGFGVLFTGSSIGGMLAPPIASALFAAH
                     GWRVAFLTSALIGLVWLPVWLFWTRQRRVAEQLDTAAQPKEAIPRPPLRELIRNPELH
                     RGLFAIFAVAPVFGVALSWGAIYLTSTWKVSQAGVGEYLWLPPLIFDGAAILFGHLAS
                     QQHRPHGEPPRMLFAIGMLLATTLVALPWMQGAWPAVYLLGIAMAGGGIVYTLATADM
                     LQRMPPSSVSFAAGTMAGAQSLALVIVNPLIGLAYDYYRNFDVVAIAIGLWSVPGCLV
                     WLLWRPRLER"
     gene            complement(1049..1678)
                     /locus_tag="VFV99_12085"
     CDS             complement(1049..1678)
                     /locus_tag="VFV99_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060096.1"
                     /translation="MAKRAWWVTLVIVSAVACSKAKDNGGAAAGSAQGSAAAGSAAAP
                     AGSATAPAGSAAAAPTGSAAEAGAAPAAGSGSAVAEGFKVGDPVMGQWTDGDWYPGKI
                     GKINDDGTYRVNYNDGDVSPRLTAKQVKPRKTGGGGGGKATASKGGEEPCPASHWTRC
                     NGNCVPLQDDRYNCGACGNVCPKNLWICRNGKCDCSANEKDSDGNCPPP"
     gene            1709..2464
                     /locus_tag="VFV99_12090"
     CDS             1709..2464
                     /locus_tag="VFV99_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5060097.1"
                     /translation="MPYARDLYYRDLGAGGPPIVALHGGWGYEFYPFDDAIAGIARRF
                     VIPDRSGYGKSPPRTSLPPRFHQLYADETTALLDALAIDRCVLWGHSDGAVIAANLAI
                     RDPARYAGIVVEAIHLDREKPRSRQFFETMARDPDKFGAGVTKKFAAEHGESWRTIIR
                     ADGEAWLHIAAHPDDDLYDNRLHELAVPMLVLHGADDPRTEPGELDRIRRDVPHAEIH
                     MLPGAGHSPHSERGSAATCTAIVARWLDVLGMA"
     gene            2464..3402
                     /locus_tag="VFV99_12095"
     CDS             2464..3402
                     /locus_tag="VFV99_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5060098.1"
                     /translation="MRLAHCSDLHLLSHDGARWLDLANKRWIGAMNLLSNRSRHYHVD
                     AFDHMVDDMNAQGIEHVLCTGDVTNLALEQEFKFARGKFDRLAGGPDHVTVIPGNHDA
                     YVAEGIAHFATVFDEYHRADTDWQWTDADRDVDDPDDDLRWPIVRVRGELALIGVSTS
                     RATPWFTAYGRIGAGQLARLRKALSDPRLANRARLVAIHHPPAGKRAANKIRGLRDHT
                     SFAQVIAEVGADLIVHGHEHQDLTDQLLGPAGPIPVRGIASGTYHHNKPDRISRYRVF
                     EIAGGKFVSDYVRVWNRDSGVFEAHPGSPQQSNYVS"
     gene            3436..4731
                     /locus_tag="VFV99_12100"
     CDS             3436..4731
                     /locus_tag="VFV99_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5060099.1"
                     /translation="MTDDLKDPASIEAADKKYVMRPWTHPAGEPVIVAKAKGCTITDV
                     HGKEYLDLTAGYFVNAAGHCHERIKEAATKQLGEVLQVSGKHGTVAAVRLAKKVVELA
                     PKSIAKVFFTTGGSEANENALRAARQFTKKTDVAYLENGYHGLTLGALEVTASKQYRE
                     SAGKPLGPENYAIPSAYCYRCKYGPHKECNVQCLDGVDKMLDARPTAAIIAEPIQAVG
                     GLAPPQKWWERLDEMRKARGILLIADEVQTGLGRTGKMFAVEHYGLQPEIMTAGKGLS
                     GGVGSLAAMCANDEISKLYFGGTTPTSGGNAVSAAAGLELISILQDEKLVENAANIGR
                     YFTEAAWALKDPWIGDVRFTGLLGGIELVKDRESREVLPKPDVGKVKDGLHAAGMLIT
                     VSGMYGNVLRLQPPLSFTTAMVDQFIGALRKVLTAVRGS"
     gene            4779..5465
                     /locus_tag="VFV99_12105"
     CDS             4779..5465
                     /locus_tag="VFV99_12105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5060100.1"
                     /translation="MPNLFAFRKNLDRVLHPLVSALARLPLHANTWTMFGALLGLIGG
                     VLFFYAHWWSGFVLLIVRGLVDHIDGFKARNFNQRSTFGAVMDDVCDRWVLGIMYAGG
                     CLNLSFDYPHVLIVLGFGITGALTNVIIKLSIYAESQQDIWREKGKIGHPVDVVGLFG
                     SAEFLIYYGTGVFFTALLNDPRPLLAGCWAVAIMSHVSLLQRVRFAWKRYRFVDPGLV
                     VADDDQAKAG"
     gene            5466..6068
                     /locus_tag="VFV99_12110"
     CDS             5466..6068
                     /locus_tag="VFV99_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5060101.1"
                     /translation="MSTATVAAPAAPGASGVTMQTPRKVSPFNPANAVTASRFLTLPP
                     LYWAIENGHHQYATLFILICGLLDKLDGLVAKVFNCRSAFGELFDAITDGICYGFGLI
                     VVAAFGWAPWIPVAAIIFLGVSNTLMRFVYVRRAGRATNYKSYAMERLVAYTGYLIGF
                     ATGGMEVKFYYWAFVPLMAVIIAYDTKRMLIDPIPPARPA"
     gene            6065..6289
                     /locus_tag="VFV99_12115"
     CDS             6065..6289
                     /locus_tag="VFV99_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060102.1"
                     /translation="MNELMSPNAFKWFLTILTGGLAGVWFFYDSFNLIRTRNLDRSDA
                     IVRDKHFGYVIGILIGIIGVVGCLRFHGVV"
     gene            complement(6266..7099)
                     /locus_tag="VFV99_12120"
     CDS             complement(6266..7099)
                     /locus_tag="VFV99_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5060103.1"
                     /translation="MRSSAEDSNVSGTPRHKDDGVRALPTGGPGVTWLGHATAVAELG
                     AARVVFDPLLPWRARAAGRVNAILITHSHVDHLNRWSLKAIDRDTQLVVPMGCMDIVK
                     DLGFAKVTEVTAGDQLDIDGLEIEAVATRHDNGRWRKGDRPECVGYVVGKGGHRIHHA
                     GDVDFSDHSVFDDIGKKFTIDATLLPIGGMLPVWYYRARRHLHDQGVHIDPDCALDIY
                     ERLGARTMVPVHWGTLHLRLGLPSMPRRRLAKIVNERNRAGVHILAHGERLSLHDAVE
                     A"
     gene            7098..7799
                     /locus_tag="VFV99_12125"
     CDS             7098..7799
                     /locus_tag="VFV99_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5060104.1"
                     /translation="MFDVAAVVTLLYSTGQPVLLFLFLGVAAGALTGVPIGPVNVAVI
                     DASYRHTMRRAIGVGLGGAIADAFYAGLGIIGVTPVIAKFPSVPPILYSVSGIVLLVY
                     GFLTARSQPVTPAVPEEPISASQVDLRAKKELGQGMKVGVALIVLNPAAMVTWVVIIG
                     QLLPDTETNWEGLACAIGVFAGSFGWFALVAYLTHKGKHVLGDKAAWIPRVVGIALMG
                     YALYLLFKAVKIVLA"
     gene            7860..8810
                     /locus_tag="VFV99_12130"
     CDS             7860..8810
                     /locus_tag="VFV99_12130"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5060105.1"
                     /translation="MRTLGWLVMVTVAGCAGDGGPASVAFVTPGTGTAFTRDHLGTSG
                     ALVANVEVEVAVEGDVARVAIAVGDGTLGYVADNKLAAEVAQSGAVTLTATALDDTDI
                     PLATATVDITIDDPMVADCKGWLDLYQLQYTSGPAMQGVADPVTVTLPLNGVAYRYSG
                     NTSQRKTLFGDCTLMKSLAQAAPIVRSHDVQEIIDIGIYNYRCIDQTMTPPNCSMSQH
                     AYAKAIDLAEFVTGDGTHYSVLNDWVIDSTGTTCTATTENDKDAWLHQLICELKGAHT
                     WNIVLTPNYNADHRNHFHVDMTKDSDFIKRAHDDSAPLDF"
     gene            complement(8807..>9410)
                     /locus_tag="VFV99_12135"
     CDS             complement(8807..>9410)
                     /locus_tag="VFV99_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5060106.1"
                     /translation="LPVAEVDENIELAQMAEHSATTAPHKKSHASLILIAGVAFVATA
                     GGVTWWLSQRQHRPHQEEAAAVPVEPAPAPAPAATATAPAPAQEPAPTPPPPPVEAPA
                     PTGVMLQIDSVPPGAAITIDGKPRGVTPLQVKLYEPRTVKLALALAGYVSVKQELAID
                     HDQRVLIPLAPKPKAAVAKPAAKKKDPKKKDPKDPFQRFD"
BASE COUNT         1538 a         3142 c         3019 g         1711 t
ORIGIN      
        1 atcgcgacgc cgctgtcggg ctggtggatc gatcggatcg gtgcccggcg cgggctcgtc
       61 gcgtcggtcc tgctgtggac cgccgttgcc gcgctgcacg cactcattcc cggcttctgg
      121 gtgttgttcg cgctgcgcat cgcgctcggc atcgccgaag gaccgagctt tccgggcgcc
      181 gcgcagacgg tgcaacgaat cctgccgccc gccgatcgcg cgcgcggctt tggcgtgttg
      241 ttcaccggct cgtcgatcgg cggcatgctc gcgccgccga tcgcgagcgc gctgttcgct
      301 gcgcatggct ggcgggtcgc gttcttgacc agcgcgctga tcggcctcgt gtggctgccg
      361 gtgtggctgt tctggacgcg gcagcgtagg gtggcggagc agctcgatac cgccgcgcaa
      421 ccgaaggaag cgatcccacg gccgccgctg cgcgagctga ttcgcaatcc cgagctgcat
      481 cgcggcctgt tcgcgatctt tgcagtcgcg ccggtgttcg gcgtcgcgtt gtcgtggggt
      541 gcgatctatt tgacgtcgac gtggaaggtc tcgcaggccg gcgtcggcga gtacctctgg
      601 ctgccgccgc tcatcttcga cggtgccgcg atcctgttcg gtcacctcgc atcgcaacag
      661 catcggccgc acggcgagcc gccgcgcatg ctgttcgcga tcggcatgct gctcgcgacg
      721 acgctcgtcg cgttgccatg gatgcagggc gcatggccgg ctgtgtactt gctcggcatc
      781 gcgatggccg gcggtggcat cgtctacacg ctcgcaaccg cggacatgct gcagcgcatg
      841 ccgccgagca gcgtgtcgtt cgcggcgggc acgatggccg gtgcgcagtc gctcgcgctc
      901 gtcatcgtca atccgttgat cggcctcgcg tacgactact acaggaactt cgatgtcgtc
      961 gcgatcgcga tcggactgtg gtcggtgcct ggctgcctcg tgtggctgtt gtggcgcccg
     1021 cgactcgagc gctagaagcg gaccaggctt acggaggagg gcagttaccg tcgctgtctt
     1081 tttcgttggc cgaacaatcg cacttgccgt tgcgacagat ccacaggttc ttggggcaca
     1141 cgttgccgca cgcgccgcag ttgtagcggt cgtcttgcag cggcacacag ttgccattgc
     1201 agcgcgtcca gtgcgacgcc gggcacggct cctctcctcc cttgctcgca gtcgccttgc
     1261 caccgccacc accgcccgtc ttgcgcggct tcacctgctt cgcggtcagc ctcggcgaca
     1321 cgtcgccatc gttgtagttc acgcggtacg tgccgtcgtc gttgatcttg ccgatcttgc
     1381 cggggtacca atcgccgtcg gtccactgac ccatgaccgg gtcaccgacc ttgaagccct
     1441 cggcgacggc ggagccactg ccggccgcag gcgccgctcc agcttcggcc gccgatccgg
     1501 tcggagccgc cgcagcgcta ccggccggtg cggttgcact tcccgccggc gcggctgcgc
     1561 ttcccgccgc cgcacttcct tgcgcgcttc ccgccgcggc gccgccgttg tccttcgcct
     1621 tgctgcaagc aactgccgaa acgatcacca gtgtcaccca ccacgcacgc tttgccatgc
     1681 gccacgatat cactggtatg actgtcgcgt gccgtacgcc cgcgacctct attacagaga
     1741 cctcggcgcg ggggggccac cgatcgttgc gctgcatggc ggctggggct acgagttcta
     1801 tccgttcgac gacgcgatcg ccggcatcgc gcggcgcttc gtgattccgg atcgcagcgg
     1861 gtacggcaag tcgccaccgc gcacgtcgct accaccgcgc tttcaccagc tctatgccga
     1921 cgagaccacc gcgctactcg acgcgctcgc gatcgatcgc tgcgtgctgt ggggccatag
     1981 cgatggcgca gtcatcgccg cgaacctcgc gatccgcgat cctgctcgct acgcgggcat
     2041 cgtggtcgaa gcgatccatc tcgatcgcga gaagccacgc tcgcgccagt tcttcgagac
     2101 gatggcgcgc gatccggaca agttcggcgc tggcgtgacc aagaaattcg ccgccgagca
     2161 cggcgagagc tggcggacga tcatccgcgc tgatggcgag gcgtggctgc acatcgctgc
     2221 gcaccccgac gacgatctct acgacaatcg cctgcacgag ctcgccgtcc cgatgctcgt
     2281 gctgcacggc gccgacgatc cacgcaccga gccgggcgag ctcgaccgca tccgccgcga
     2341 cgtgccacat gccgagatcc acatgcttcc cggcgccggt cacagccccc atagcgagcg
     2401 cggctcggcg gccacgtgca cggcgatcgt cgcacgctgg ctcgacgtgc taggtatggc
     2461 gtgatgcgcc tcgcgcactg ctcggacctt catctgttgt cgcacgatgg tgcgcgctgg
     2521 ctcgatctcg caaacaagcg ctggatcggc gcgatgaatt tgctctcgaa ccgcagccgc
     2581 cactatcacg tcgacgcgtt cgaccacatg gtcgacgaca tgaacgcgca aggcatcgag
     2641 cacgtgctgt gcaccggcga tgtcacgaac ctcgcgctcg agcaggagtt caagttcgcg
     2701 cgcggcaagt tcgaccggct cgccgggggc cccgaccacg tcaccgtcat tccgggtaat
     2761 cacgacgcgt acgtcgccga gggcatcgcg cacttcgcga ccgtgttcga cgagtaccac
     2821 cgcgccgaca ccgactggca gtggaccgac gcagaccgcg acgtcgacga tcccgacgac
     2881 gatctgcgat ggccgatcgt tcgcgtgcgc ggcgagctcg cgctgatcgg cgtgtcgacg
     2941 tcccgcgcga cgccgtggtt caccgcgtac ggccggatcg gcgcagggca actcgcgcgt
     3001 cttcgcaagg cgctttccga tccgcggctc gcgaaccgcg cacggctcgt cgcgattcat
     3061 catccacccg ccggcaaacg tgccgcgaac aagatccgcg gccttcgcga tcacacgtcg
     3121 ttcgcgcagg tgatcgccga ggtcggagcc gatctgatcg tccacggtca cgagcaccag
     3181 gacctcaccg atcagctcct cggaccggct ggaccaattc cggtgcgcgg catcgcctcc
     3241 ggcacctacc accataacaa gcccgatcga atcagccggt acagagtctt cgagatcgcg
     3301 ggcggcaagt tcgtgagcga ttacgtaaga gtctggaatc gggacagtgg cgtttttgag
     3361 gcacaccccg gcagccctca gcaatctaac tatgtaagtt gacaacggtt tgaggcatga
     3421 gatacctcac gcaaagtgac ggacgatctc aaagaccccg cgtcgatcga agccgccgac
     3481 aagaagtacg tgatgcgtcc ctggacgcat ccagccggcg aacccgtgat cgttgccaag
     3541 gccaagggct gcacgatcac tgacgttcac ggcaaggagt atctggacct gacggccggc
     3601 tacttcgtca atgccgcggg tcactgtcac gaacggatca aagaagccgc gaccaagcag
     3661 ctcggcgaag tgctgcaggt ctccggcaag cacggcacgg tcgccgcggt acggctggcg
     3721 aagaaggtcg tcgagctcgc gccaaagtcg atcgcgaagg tgttcttcac gacgggtggc
     3781 tccgaggcga acgagaatgc gctccgcgcc gctcgccaat tcacgaagaa gaccgacgtc
     3841 gcgtatctcg agaacggcta ccacggcctc acgctcggcg cgctcgaggt gacggcgagc
     3901 aagcagtatc gcgagagcgc cggcaagcca ctcggccccg agaactacgc gatcccgtcg
     3961 gcgtactgct atcgctgcaa gtacggcccg cacaaggaat gcaacgtcca gtgcctcgac
     4021 ggcgtcgaca agatgctcga cgcgcggccg accgccgcga tcatcgccga gccgatccaa
     4081 gcggtcggtg gtctcgcgcc gccacagaag tggtgggagc gcctcgacga gatgcgcaag
     4141 gcgcgcggca ttctgctcat cgccgacgaa gtgcagaccg gcctcggtcg caccggcaag
     4201 atgttcgccg tcgagcacta cgggcttcag cccgagatca tgacggcagg caagggcctg
     4261 tctggcggcg tcggctcgct cgctgcgatg tgcgcgaacg acgagatcag caagctgtac
     4321 ttcggcggca cgacgccgac gtcgggcggc aacgcggtct ccgcggcagc tggcctcgag
     4381 ctcatcagca tcttgcagga cgaaaaactc gtcgagaacg cggccaacat cggccggtac
     4441 ttcaccgagg cggcgtgggc gctcaaggat ccatggatcg gagatgttcg cttcacgggg
     4501 ctcctcggtg gcatcgagct cgtgaaggat cgcgagtcgc gcgaagttct gccgaagccc
     4561 gatgtcggca aggtcaaaga cggtctgcac gccgcgggca tgctgatcac cgtctcgggc
     4621 atgtatggca acgtgctgcg cctacagccg ccgttgtcgt ttacgaccgc aatggtcgac
     4681 cagttcattg gcgcgctgcg caaggtactt accgcagttc ggggcagctg atccccaggg
     4741 ccaagccccc cgtattcgga tagagtccgt acccgcagat gccaaacctg ttcgcctttc
     4801 gcaaaaacct cgatcgggtg ctccatccgc tcgtgtctgc gctggccagg ttgccgctcc
     4861 acgcgaacac gtggacgatg ttcggcgcgc tgctcggcct gatcggcggc gtgttgttct
     4921 tctacgcgca ctggtggtcg ggcttcgtgc tgctcatcgt gcgcggtctc gtcgatcaca
     4981 tcgatggctt caaggctcgc aacttcaacc agcgcagcac attcggcgcg gtgatggacg
     5041 acgtctgcga tcgctgggtg ctcggcatca tgtacgccgg cgggtgcctc aacctgtcgt
     5101 tcgactaccc gcacgtcttg atcgtgctcg gcttcggcat cacgggcgcg ctgacgaacg
     5161 tcatcatcaa gctgtcgatc tacgcggaga gccagcaaga catctggcgc gagaagggca
     5221 agatcggtca cccggtcgac gtcgtcggct tgttcggctc cgccgagttc ctcatctact
     5281 acggcaccgg cgtgttcttc accgcgctgc tcaacgatcc gcgtccgttg ctcgcaggtt
     5341 gctgggccgt cgcgatcatg tcacacgtgt cactgctcca gcgcgtacgc ttcgcgtgga
     5401 agcgttaccg cttcgtcgat ccgggcctcg tggtcgccga cgacgatcaa gcaaaggctg
     5461 gatagatgtc gactgcgact gttgccgccc cggctgcccc cggcgcctcc ggcgtgacca
     5521 tgcagacccc tcgcaaagtc tcgccgttca acccggccaa cgcggtcact gcgtcgcgtt
     5581 ttttgacgtt gccgccgctc tactgggcga tcgagaacgg tcaccaccag tacgcgacgc
     5641 tgttcattct gatctgcggc ctcctcgaca agctcgacgg gctcgtcgcg aaggtcttca
     5701 actgccggag cgcgttcggc gagctgttcg acgcgatcac cgatggcatc tgctacggct
     5761 tcgggctcat cgtcgtcgcc gcgttcggct gggcgccgtg gattccggtc gccgcgatca
     5821 tcttcctcgg cgtctcgaat acgctgatgc ggtttgtcta cgtccgccgc gccggccggg
     5881 ctacgaacta caagtcctac gcgatggagc ggctcgtcgc gtacacgggc tatctgatcg
     5941 gctttgcgac cggcggcatg gaagtgaagt tctactactg ggcgttcgtg ccgctgatgg
     6001 ccgtcatcat cgcctacgac accaagcgca tgctcatcga tccgattcca ccggcgaggc
     6061 ccgcatgaac gagctgatgt cgccgaacgc gttcaagtgg ttcttgacga tcctcaccgg
     6121 cgggctcgcc ggcgtgtggt tcttctacga ctcgttcaac ctgattcgca cgcgcaacct
     6181 cgatcgcagc gacgcgatcg tgcgcgacaa acacttcggc tacgtgatcg ggatcctgat
     6241 cgggatcatc ggtgtggtcg gctgtctacg cttccacggc gtcgtgtagc gacagccgct
     6301 cgccgtgcgc gaggatgtgc acgccggcac ggttgcgctc gttgacgatc tttgcgagcc
     6361 ggcgccgcgg catcgacggc agcccgagcc gcaggtgtag cgtgccccag tgcaccggca
     6421 ccatcgtgcg ggcgccaagt cgctcgtaga tgtcgagcgc gcagtcggga tcgatatgca
     6481 cgccttggtc gtgcaggtga cggcgcgcgc ggtagtacca aacgggcaac atgccgccga
     6541 tcggtagcag cgtcgcatcg atcgtgaact tcttaccgat gtcgtcgaac accgagtgat
     6601 cggagaagtc gacgtcgccg gcatggtgga tgcgatggcc gcctttgccg acgacgtaac
     6661 cgacacattc gggcctatcg cccttacgcc agcgcccgtt gtcgtggcgg gtcgccaccg
     6721 cttcgatctc gagcccgtcg atgtcgagct gatcgccggc ggtgacctcg gtgaccttcg
     6781 cgaagccgag gtctttgacg atgtccatgc agcccatcgg tacgaccagc tgcgtgtcgc
     6841 ggtcgatcgc cttgagactc cagcgattca agtgatcgac gtgggagtga gtaatcaaga
     6901 tggcgttgac gcgacccgcg gcgcgggcac gccacggcaa cagcgggtcg aacacgacac
     6961 gcgccgcacc aagctcggca accgcggtcg cgtgaccgag ccacgtgaca ccggggcccc
     7021 ccgtagggag tgcccgtacc ccatcatcct tatgacgcgg tgtgcccgaa acattcgaat
     7081 cttcggcgga agatcgcatg ttcgacgtgg cggccgtggt cacgctgctg tatagcacgg
     7141 gacagcccgt gctcctgttc ctgttcctag gcgtcgccgc aggtgcgctg accggtgtgc
     7201 cgatcgggcc agtgaacgtc gcagtgatcg acgcgtcgta ccggcacacg atgagacgcg
     7261 cgatcggagt gggactcggc ggcgcgattg ctgacgcgtt ctacgcagga ctcggcatca
     7321 tcggtgtgac gcccgtgatc gcaaagtttc catcggtgcc accgatcctc tactcggtca
     7381 gcggcatcgt gttgctcgtc tacggatttt tgaccgcgcg tagtcagccg gtcacgcccg
     7441 cggttccgga ggaaccgatc tctgcatcgc aagtcgacct gagggcgaag aaggagctcg
     7501 gccaagggat gaaggtcggc gtcgcgctca tcgtgctgaa cccggcggcg atggtgacgt
     7561 gggtcgtgat catcggccag ctgttgcccg acaccgagac caactgggaa gggctcgcgt
     7621 gtgcgatcgg cgtgttcgcc ggcagcttcg ggtggttcgc gctcgtcgcg tacttgaccc
     7681 acaagggcaa gcacgtgctc ggcgacaagg ccgcgtggat cccgcgcgtc gtcggcatcg
     7741 cgctcatggg ctatgcgctc tacttgctgt tcaaagcagt gaaaatagtc ctcgcgtagg
     7801 tggcagatcc gattgccgct tgaccgcggt tcatcgtcgg tgttctctac gccgcacgga
     7861 tgcgtaccct cggttggctc gtgatggtga cggtggcggg ttgcgccggc gacgggggac
     7921 ccgcatcggt cgcgttcgtc acgcccggca cgggcacagc gttcacccgt gatcacctcg
     7981 gcacgtcggg tgcgctggtc gcgaacgtcg aggtcgaggt cgcggtcgag ggtgacgttg
     8041 cacgcgtcgc gatcgcggtc ggtgatggca cgctcggtta cgttgccgac aacaagctcg
     8101 ccgccgaggt cgcccaatcc ggtgcagtga cgctcacggc gaccgcactc gacgacaccg
     8161 acattccgct cgcgactgcg accgtcgaca tcacgatcga cgatccgatg gtcgccgact
     8221 gcaaaggctg gctcgatctc tatcagctcc agtacacgag cggccccgcg atgcagggcg
     8281 tcgccgatcc ggtgacggtc acgctgccgc tcaacggcgt cgcgtaccgc tacagcggca
     8341 acacctcgca gcgcaagacg ctgttcggcg actgcacgct catgaagtcg ctcgcgcagg
     8401 cagcgccgat cgtgcgcagc cacgacgtgc aggagatcat cgacatcggc atctacaact
     8461 atcgctgcat cgatcagacg atgacgccac cgaactgctc gatgtctcaa cacgcgtatg
     8521 cgaaggcgat cgatctcgcc gagttcgtca ccggcgacgg cacgcattac tcggtgttga
     8581 acgactgggt gatcgactcg accggcacga cgtgcaccgc cacaaccgag aacgacaagg
     8641 acgcgtggct acaccagctg atctgcgagc tcaaaggcgc gcacacgtgg aacatcgtgc
     8701 tgacgccgaa ctacaacgcc gatcaccgca accacttcca cgtcgatatg acgaaagact
     8761 ccgacttcat caagcgcgcg cacgacgaca gcgcgccgct cgacttctaa tcgaagcgct
     8821 ggaacggatc cttcggatcc ttcttcttcg gatccttctt cttcgctgct ggcttcgcga
     8881 ccgcggcctt tggcttcggc gcgagtggaa tcagcacgcg ctgatcgtga tcgatcgcga
     8941 gctcctgctt gacgctgacg tacccggcga gcgccagcgc gagcttcacc gtgcgcggct
     9001 cgtagagctt cacctggagc ggcgtcaccc cacgcggctt gccatcgatc gtgatcgccg
     9061 caccgggcgg cacgctgtcg atttgcagca tgacgcccgt aggcgcgggc gcctcgaccg
     9121 gtggcggtgg cggcgtcggc gctggctcct gcgctggcgc gggcgccgta gccgtggccg
     9181 ctggcgccgg cgcaggcgcc ggctcgacgg gaacggcggc tgcctcctcc tgatgcgggc
     9241 gatgctggcg ctgcgagagc caccaggtga cgccaccggc ggtcgcgacg aatgcgacgc
     9301 cggcgatcag gatcagagat gcgtggctct tcttgtgtgg cgcggtggtc gcgctgtgct
     9361 cggccatttg cgcgagctcg atgttctcgt cgacctcggc gaccggcagc
//
LOCUS       DASUFX010000308         6673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_464435,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000308 DASUFX010000000
VERSION     DASUFX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464435"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="VFV99_12140"
     CDS             complement(<1..1994)
                     /locus_tag="VFV99_12140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX5060107.1"
                     /translation="MRKRRALVAACLVLSCGGPQRPSSKNEPPLEVKTYGPLGIKADA
                     KMPNRAVILGTDVKDGSTIVPLPVAQGKTVVDAMFVNMSNPKNVTGGMSPVKLATAPN
                     SDGSVQVGIFEEFAGGTGPQWRAGVWVSAFVAATTLNKDLTDFTFSATSGGYIDGASA
                     SGLMAAGFLAAMTGATVDPTVTMTGIINPDGTFGPVDGIPEKLLSAIEKGKKRVGYPI
                     GMRNATSAKTGETVDLVALAKEHGAEAVEIADVHEAYKLLTGKTLPEPIAVNESDMAL
                     DASTDKALDAKYKVWQQRLATEWGAILQLESAGRLPALLVYLRDYAKRYAEAAEQLHK
                     DGKLAAAYARMLAAAVYATSANQIYDVLANVQANKLDAAVASLDKLDQLDQLTKDTLT
                     KVGTLRPPTMGGHLQMMAAFRSALRGWVFKDFASLALTSTKQYIKSLAGTPAATLGSE
                     KTADSVVAQVGPTVLYVAKTTAETTLATEQLEFANANDISYMCSVPNVLRLATSFQSA
                     GAAGMNYFDTLLVEPFAQSEHIPVDDARTRVAILEPDYLVAIMTSKLGSLDGTPKELR
                     SKWGDKSLAWGLMSLAGSELAYFHAAELIAKYYSLDVRLDDARQVNHVEHEKAFTNML
                     ATAERNARASARAARIATGAIPVQAKLAYQLASVERAGNLA"
     gene            complement(2009..2920)
                     /locus_tag="VFV99_12145"
     CDS             complement(2009..2920)
                     /locus_tag="VFV99_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5060108.1"
                     /translation="MARRLRLDEAVDMFLDHLKVERGLSAHTLDGYGRDLAKLSQFLT
                     ARGREDVDDVSAGDITDHLIELAENKLAPRSRARALVAMRGLFRHLVGERWLEADPTA
                     LIDTPRTARRLPGVLGEDAVARLIAQPKDTPRGRRDAAMIELAYASGLRVSELVHIAL
                     ADINLNHGFVRITGKGNKTRLVPVGSAARDKIAQYLEHDRPRFVRDPAERALFLTERG
                     RPMTRQGFWKSLRNYARRAGVRLPAGDISPHKLRHSFATHLVERGADLRAVQAMLGHA
                     DISTTEIYTHVSRARVLEQARKHPRAR"
     gene            3016..3717
                     /locus_tag="VFV99_12150"
     CDS             3016..3717
                     /locus_tag="VFV99_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5060109.1"
                     /translation="MNLSPEDIRQIVIRVFVLVVSVCFHEFGHAYMADRLGDDTPRRQ
                     GRVTLNPLAHADPIGTLLLPIVGAIYAAGGHSIGGFGWGRPVQWQPSRVARKWKMSTA
                     RILVAVAGPSMNVVLAVLVAGIQSVLISQGVIDPFGRASQIMFIAVGTNFILFFFNLV
                     PAPPLDGGHVAEGFMPYKHRSTFEQYARFGPFIVLAIAMISPLAQIFVIPAKFVASKL
                     YLLMGQDLLAMLMNS"
     gene            3717..4550
                     /locus_tag="VFV99_12155"
     CDS             3717..4550
                     /locus_tag="VFV99_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX5060110.1"
                     /translation="MDAGYQVNLDVFEGPLDLLLHLVKKHELSILDIPIAFVTEKYLE
                     YLDAMAGLDIDVAGEYLLMAATLCHIKSRELLPSSEPLDEEGEAVEGEEVEVDPRGDL
                     IKRLLEYQKYKEAADKLGQRPVVGRNVWGRGTPTEDAVSEGVDAEAIAPLAPFPVHKL
                     IEAFDRVMQQAKIKVAHNVMIDRLSVSQRISELTDRLETEGRFAFSSMFSFLRDGVER
                     TLEEVRHEAVVTFLALLEMAKLRLVALSQHEDEEIFIQRAADDLRARMEQTVARSDDY
                     R"
     gene            4613..>6673
                     /gene="scpB"
                     /locus_tag="VFV99_12160"
     CDS             4613..>6673
                     /gene="scpB"
                     /locus_tag="VFV99_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5060111.1"
                     /translation="MSRKRKKQRANRAVPEEILAEGSGPIQDADSLAEAASAALAADG
                     DEEVVFEAADAVEASSAEATEAPSAEAAEPAEDDSGKTKGKSKSKGRRGKRLEVTSDE
                     PMIEQVATEAADEAVIEAAALEAAVEADVLDSEVGPEIEEAEVAAEQAAQADEQVLED
                     AVMRAEAEAELGAAEPEAEAGEEDVELGAALPTTAGTMDDLQLKHLVEALIFAADKPV
                     TMQRLRQLTRVSDVKRLEQALATLATDFADRGIALQQVSGGYQFRTNTQYSTWVQQLI
                     AGRPVRLSRAQLETLAIVAYRQPITRPEVDEIRGVDSSATLRLLMDRALIRILGKKED
                     VGRPILYGTTKEFLDFFSLGDLRELPTLREYSELTAESRQVVSDRLGLDAEPPSVDGV
                     SEDGELAEPDDANVVVAQEMSVDDMVREFATSDVDAPSSTDDVALGATDVASDELEMT
                     GADVASDELDMAGTDVASIELDMAGTDVASDELDMTGAGSASDELDMAGTDVASIELD
                     MAGTDVASDELDMTGADAASDELDVAGAEADVAGEEGSSASDIAGDADVSGEADVASD
                     VLEAAPSDADAASEVLEAAASDADAASDVVEAPAAADVAANDVAGAQADIAGDADSSA
                     ASLAGDADVAGDADVAGNADVAGNADGAGDSLDAAADALVAAADDAAADEAAAGQAAM
                     DEAVA"
BASE COUNT         1101 a         2095 c         2329 g         1148 t
ORIGIN      
        1 gcgaggttgc ctgcgcgttc gacgctcgcg agttgatacg cgagcttggc ctgcaccgga
       61 atcgctcccg tcgcgatgcg tgcggcacgg gcgctggccc gcgcgttgcg ctcggcggta
      121 gcgagcatgt tcgtgaacgc cttctcgtgc tcgacgtgat tgacctggcg tgcgtcgtcg
      181 aggcggacgt cgagcgagta gtacttcgcg atcagctcgg cggcgtgaaa gtacgcgagc
      241 tcgctgccgg caagcgacat caaaccccac gcgagcgatt tgtcgcccca cttgctccgc
      301 agctccttcg gcgtgccgtc gagcgagccg agcttcgacg tcatgatcgc gacgagataa
      361 tcgggttcga ggatcgcgac gcgcgtacgc gcatcgtcga ccgggatgtg ctcgctctgc
      421 gcgaacggct cgacgagtag cgtgtcgaag tagttcatgc ccgccgcgcc cgccgattga
      481 aacgacgtcg cgagccgcag cacgttcggc accgagcaca tgtaactgat gtcgtttgcg
      541 ttcgcgaact cgagctgctc ggtcgcgagc gtcgtctccg cggttgtctt cgcgacgtag
      601 agcaccgtcg gtccgacttg cgcgacgacg ctgtccgccg tcttctccga gccgagcgtc
      661 gcggccggtg tgccagcgag tgacttgatg tactgcttcg tgctcgtcag cgcgagtgac
      721 gcaaagtcct tgaacaccca gccgcgcagc gccgaacgga acgccgccat catctgcagg
      781 tggccgccca ttgtcggcgg gcggagcgtg ccgaccttcg tgagcgtgtc cttcgtgagc
      841 tggtcgagct gatcgagctt gtcgagcgat gcgactgcgg catcgagttt gttcgcctgc
      901 acgttcgcga gcacgtcgta gatctggttc gcactcgtcg cgtagaccgc cgcggccagc
      961 atgcgcgcgt acgcggcggc gagcttgccg tccttgtgca gctgctcggc cgcctcggcg
     1021 tagcgcttcg cgtagtcgcg caagtaaacg agcagggcag gcaaccgacc tgcgctctcg
     1081 agctgcagga tcgcacccca ctcggtcgcg agacgctgct gccagacctt gtacttcgcg
     1141 tcgagcgcct tgtccgtcga cgcgtcgagc gccatgtcgc tctcgttgac cgcgatgggc
     1201 tcgggcagcg tcttgccggt gagcagcttg tacgcttcgt gaacgtctgc gatctcgacg
     1261 gcctcggcgc cgtgctcctt cgcgagcgcg acgagatcga cggtctcgcc ggtcttcgcc
     1321 gacgtcgcgt tgcgcatgcc gatcggatag ccgacgcgct tcttgccctt ttcgatcgcc
     1381 gacaggagct tctccggaat cccgtcaacg gggccgaacg ttccgtcggg gttgatgatg
     1441 ccggtcatcg tcacggttgg atcgaccgtc gcgcccgtca tcgcggcgag aaagcccgcg
     1501 gccatcaggc ccgatgccga tgcgccatcg atgtagccgc cgctcgtggc cgagaacgtg
     1561 aagtcggtga ggtctttgtt gagcgtcgtc gcggcgacaa acgcgctcac ccagacgccc
     1621 gcgcgccact gcggtcccgt gccgccggcg aactcttcga agatgccgac ctgcaccgag
     1681 ccgtcgctgt tcggcgcggt cgcgagcttc accggtgaca taccgccggt gacgttcttc
     1741 ggattgctca tgttgacgaa catcgcgtcg acgacggtct tgccctgcgc gaccggcagc
     1801 ggaacgattg tcgagccgtc cttcacgtcg gtaccgagga tgacggcgcg gttcggcatc
     1861 ttcgcgtcgg ccttgatgcc gagtggcccg tacgtcttca cctcgagcgg tggctcgttt
     1921 ttcgacgacg gacgttgcgg cccaccgcac gacagcacga gacatgcagc gacgagcgcg
     1981 cggcgcttcc tcatccgggg agactaactc accgagcgcg cggatgcttg cgcgcttgct
     2041 ccagcacgcg agcgcggctg acgtgtgtgt aaatctctgt cgtcgatata tcggcgtggc
     2101 cgagcatcgc ctggaccgcg cgcaggtcgg cgccgcgctc gacgaggtga gtcgcgaacg
     2161 agtgacgcaa cttgtgcggc gagatgtctc cggccgggag ccgcacgccg gcgcggcgcg
     2221 cgtaattgcg caacgacttc cagaatccct gacgcgtcat cggccggccg cgctcggtga
     2281 ggaacagcgc acgctcggcg gggtcgcgga caaatcgcgg ccgatcgtgc tcgaggtact
     2341 gcgcgatctt gtcgcgcgcg gcgctgccga ccggcacgag tcgcgtcttg ttgcccttgc
     2401 cggtgatgcg cacgaagccg tgattgaggt tgatgtcggc gagcgcgatg tggacgagct
     2461 cggagacacg caggcccgat gcgtatgcga gctcgatcat cgcggcgtcg cggcgaccgc
     2521 gcggcgtgtc tttgggctgc gcgatcaacc tggcgacggc gtcttcaccc agcacgccgg
     2581 gcaggcggcg tgccgtacgc ggcgtgtcga tcagtgcggt cggatcggct tcgagccagc
     2641 gctcaccgac gaggtggcgg aacaacccgc gcatcgcgac gagcgcgcgg gcacggctgc
     2701 gcggtgcgag cttgttctcg gcaagctcga tcagatgatc agtgatgtct ccggcgctca
     2761 cgtcgtcgac atcctcgcgg ccgcgcgcgg tcaggaattg gctgagcttg gccagatcgc
     2821 ggccgtagcc gtcgagcgtg tgcgcggaca gcccgcgctc gaccttcagg tgatcgagga
     2881 acatatcgac ggcctcgtcg agccggagcc ggcgcgccat cccccgattg gacggtgatc
     2941 cgtgagatcg cgcaataatt tgcacgcttg cagtccgcct cgggcgcggc catattccag
     3001 cttcgagcac gcgacatgaa cctgtccccc gaagacatcc gacaaatcgt gatccgggtg
     3061 ttcgtgctcg tggtgtccgt gtgcttccac gagtttggtc acgcgtacat ggccgaccgg
     3121 ctcggtgacg acacgccgcg gcgtcagggt cgcgtgacgc tgaacccgct cgcgcacgct
     3181 gatccgatcg gtacgctgct gctcccgatc gtcggcgcga tttacgcagc cggtggccac
     3241 agcatcggtg gcttcggctg gggacggccg gtgcaatggc agccgtcgcg cgtcgcccgc
     3301 aagtggaaga tgagcacggc gaggatcctc gtcgcggtcg ccgggccgtc gatgaacgtc
     3361 gtgctggccg tgctggttgc tggcatccag tcggtcctga tcagccaagg cgtgatcgat
     3421 ccattcggtc gagccagcca gatcatgttc atcgccgtcg gcacgaactt catcctgttc
     3481 ttcttcaacc tcgtgcctgc gccgccgctc gacggtggtc acgtcgccga gggcttcatg
     3541 ccgtacaagc accgctcgac gttcgagcag tacgcgaggt tcggcccgtt catcgtgctc
     3601 gcgatcgcga tgatctcgcc cctcgcgcag attttcgtca tcccagcgaa gttcgtcgcc
     3661 tcaaagttgt atttgttgat gggccaagac ctgttggcca tgctgatgaa ctcctgatgg
     3721 acgctggcta tcaagtaaac ctcgacgtct tcgagggtcc gctcgatctg ttgcttcacc
     3781 tggtgaagaa gcacgagctg tcgatcctcg acatcccgat cgcgttcgtc accgagaagt
     3841 acctcgagta tctcgatgcc atggctggcc tcgacatcga cgtcgcgggc gagtacttgc
     3901 tgatggccgc gacgctctgc cacatcaagt cgcgcgagct gttgccgtca tccgagccgc
     3961 tcgacgaaga aggcgaagcg gtcgagggcg aagaggtcga ggtcgatccg cgcggggatc
     4021 tgatcaagcg cttgctcgag taccagaagt acaaggaagc ggcggacaag ctcggccagc
     4081 ggccggtcgt cggtcgcaac gtgtggggac gcggcacgcc gaccgaggat gcggtgagcg
     4141 aaggcgtcga cgccgaggcg atcgcgccgc tcgcgccgtt cccggtgcac aagctgatcg
     4201 aagcgttcga tcgcgtgatg cagcaggcca agatcaaggt cgcgcacaac gtgatgatcg
     4261 accgtctgtc ggtcagccaa cggatctcgg agctgaccga tcgcctcgag accgagggcc
     4321 ggttcgcgtt ttcgtcgatg ttctcgttct tgcgcgatgg cgtggagcgg acgctcgaag
     4381 aggtccgcca cgaagcggtg gtgaccttcc tcgccctgct cgagatggcc aagctgcgcc
     4441 tggtcgcgct cagccagcac gaggacgagg agatcttcat ccagcgcgcg gcggacgatc
     4501 tccgcgcccg gatggagcaa accgtcgccc gcagcgacga ctatcggtag tgtcagcgcc
     4561 tcgtgctatg aggggctagc cgcggagctt tcgcccgcgt cgccgaggtg ttgtgagccg
     4621 aaagcgcaag aaacagcgag ccaatcgagc ggttcccgag gaaatcctcg cggagggcag
     4681 tggtccgatc caagatgccg attcgcttgc ggaagccgcg agcgcggcac tcgcggccga
     4741 tggtgacgaa gaggtcgttt tcgaggctgc cgacgccgtt gaagcatcgt cggcggaggc
     4801 gaccgaagca ccgtcggcgg aggcggcgga gccggcggag gacgattccg gcaagaccaa
     4861 gggcaagtcg aagtcgaagg gcaggcgggg caagcggctc gaggtgacca gcgacgaacc
     4921 gatgatcgag caggtcgcca cggaagcggc tgacgaggcg gtgatcgagg cggcggcgct
     4981 cgaagcagcc gtcgaagcgg acgtgctcga ctcggaggtt ggccccgaga tcgaagaggc
     5041 cgaggtcgcg gccgagcagg cggcgcaggc ggacgagcag gtgctcgagg acgccgtgat
     5101 gcgcgcggaa gccgaggcgg agctaggtgc tgccgagcca gaggccgaag caggcgaaga
     5161 agatgtcgag ctgggcgcgg cgctgccgac gacggccggc accatggacg acctccagct
     5221 gaagcacctc gtcgaggcgc tgatcttcgc ggcggacaag ccggtgacga tgcagcggct
     5281 gcgtcagctc acccgcgtgt cggacgtgaa gcggctcgag caggcgctcg ccacgctcgc
     5341 gaccgatttc gccgatcgcg gcatcgcgct gcagcaagtg tcgggcggct atcagttccg
     5401 tacaaacacg cagtactcga cgtgggtgca gcagctgatc gcgggtcggc ccgtgcggct
     5461 gtcgcgcgcg cagctcgaga cgctcgcgat cgtcgcgtat cggcagccga tcacgcggcc
     5521 cgaggtcgac gagatccgcg gcgtggattc gtcggcgacg ctgcgcttgc tgatggatcg
     5581 agcgctgatt cgcatcctcg gcaagaagga agatgtcggt cgcccgatcc tctacggcac
     5641 gacgaaggag ttcttggact tcttcagcct cggtgatctg cgcgagctac cgaccttgcg
     5701 cgagtacagc gagctgaccg cagagagccg ccaggtagtg agcgatcggc tcggcctgga
     5761 tgcagagcca ccgagcgtgg acggtgtgtc ggaagatggc gagctcgccg aacccgacga
     5821 cgcgaacgtc gttgtcgcgc aagagatgag cgtcgacgac atggttcgcg agttcgcgac
     5881 cagcgatgtc gatgcgccga gctcgaccga cgacgttgcg ctcggtgcga cagatgtcgc
     5941 gagcgacgag ctcgagatga cgggcgctga tgtcgcgagc gacgagctcg atatggccgg
     6001 cactgatgtc gccagcatcg agctcgatat ggccggcact gatgtcgcga gcgacgagct
     6061 cgacatgacg ggcgctggtt ccgcaagcga cgagctcgac atggccggca ctgatgtcgc
     6121 cagcatcgag ctcgatatgg ccggcactga tgtcgcgagc gacgagctcg acatgacggg
     6181 cgctgatgcc gcgagcgacg agctcgatgt ggccggcgct gaagcggacg tggctggcga
     6241 agaaggctcc agcgcgtcgg atatcgcggg cgacgcggat gtttcgggcg aggcggatgt
     6301 cgcaagtgat gtgctcgaag ccgctccgag cgacgcggat gctgcgagcg aagtgctcga
     6361 agccgccgca agcgacgcgg atgctgcgag cgatgtcgtt gaagctccag ccgcggcgga
     6421 cgtcgcggct aatgacgtcg ctggcgcgca ggcggatatc gcaggcgatg cagactcgag
     6481 cgccgcgagc ctcgcgggcg atgcggacgt cgcgggcgat gcggacgtcg cgggcaatgc
     6541 ggacgtcgcg ggcaatgcgg acggcgcggg cgattcgctc gacgccgctg cggatgcgct
     6601 cgttgccgct gcggacgacg ctgctgccga cgaagctgcc gcgggacaag ccgcgatgga
     6661 cgaagccgtg gcc
//
LOCUS       DASUFX010000309         6929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1576640, whole genome shotgun sequence.
ACCESSION   DASUFX010000309 DASUFX010000000
VERSION     DASUFX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576640"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFV99_12165"
     CDS             <1..1181
                     /locus_tag="VFV99_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5060112.1"
                     /translation="NPQTITITSDAYLGSPQAVEVRCKIADTTLQIMPTMFDFGEVRV
                     AATAPSIPITLTNPASSAGAAMITAFDLRLHKNGLTLTKPAVPFSIAAGTVANASLSV
                     STTEDTDLTGEYLDITVDGSTLSFPVTGKVVHPHAYVTPTNMLDLGTACVGTQISGDV
                     VMTNDGTATLFVMPPVMTPSFVAAAPATPGPLPPGMSRTATVTPAMSAMGPIEGTLSW
                     TEDTPMTHEVGVKLDYVATGTALSPRGLDFGIVPVDDDPLTEYTQHLTLQNCDQAPTS
                     IKIESLKTKAGTLGAWHLVPNVGYTKQLGAKETQAVQVTFDPPARGKYEADMTVQTSS
                     GKQVVHLIGDATGRDFDNTSFYACACNGPGAPSRGWPIVLAVAIVIVRRRRPGPSSPR
                     "
     gene            complement(1138..2304)
                     /locus_tag="VFV99_12170"
     CDS             complement(1138..2304)
                     /locus_tag="VFV99_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5060113.1"
                     /translation="MADPVAESLVEYRKHFPALEECVHFISHSLGCVPAQTKEDLGEF
                     FELWRTKSITAWSDWLPEVDRAAARIAKIISAPDGTVIMHHNVSQIMATLASCFEFTA
                     PRNKVVYESLMFPTVSYVWQAEKRRGAECVVVPAKDGTMDADAICAAIDERTLLVPLQ
                     HVVFATAQIQDVKKIVARAKEVGAHVILDAYQSIGTLPIDVVDLGVSFACGGSVKYVC
                     GGPGAAWLYVRKDLIEQFAPRVTGWFGNESPFAFTMPEQSYADNVWRYMGGTPAVAAL
                     YQSRAGQSIIGEIGVRKIREKSLVMTQKCIDWVDELGLKLNSPRPAEVRGGSVVFDFV
                     GSADVCRELNRRKFFCDHRPSAGIRIAPHFYTKPDEVDLFFTEVKKVRGGGGGR"
     gene            2330..3604
                     /locus_tag="VFV99_12175"
     CDS             2330..3604
                     /locus_tag="VFV99_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060114.1"
                     /translation="MVDETVKDWITQQMALLIKRTGLDSYVAAPLVEQTDQWFPDEWH
                     PDDAGVARLGKRVFEHAGLPDVEIATELTKNTGSKGIVATHVDAKVVRLDVDPEFLTD
                     PLTIISHLISLAAVVFRVRHELEDPDHDTERRLIDLTTIYLGFGILTTNAAYRYRASG
                     ELQGSTAITRWSHDVQGSLPADAMAYALAQQLVARNASPAELRNVKRQLETNQAEVFE
                     TTCRQLKTDYVVLALDLPARNKWPAPRQPPAPPTSTALRLWKKPASNLPVATVKSTGY
                     LGFNSGRSVLRVPRNRRWEAGMIGLFVSMVPTIALAVKGFGGAAMGAMAATWLLSVML
                     GNKWVRETCSDPKCEMKLERGVQTCPRCGGTIAGTVQKGENRLEAEERLQLNQGDYDM
                     DVGDPAESGTALPSARVERERATSEGSTSTIV"
     gene            complement(3601..4005)
                     /locus_tag="VFV99_12180"
     CDS             complement(3601..4005)
                     /locus_tag="VFV99_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060115.1"
                     /translation="MLFVLGGCGFAQKHPGITVGIVAGTIGFGACEISVEKVKTCGAI
                     GAIAGVALGGITGLVTMFADTSAHELTNDDEGDVIVAPQNADEPPGLPLDAGIMAPVA
                     PVAPVAPIAPVAPTAQPDAGIPVGDAALSYGG"
     gene            4145..4744
                     /locus_tag="VFV99_12185"
     CDS             4145..4744
                     /locus_tag="VFV99_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060116.1"
                     /translation="MKYGALFAVLALSGPAFAQPGAPEPNAYWPPPDQAQQPQPQPQQ
                     NAATRATFVSSGEEKWDVWVDQQPACATPCTIGVMPLQYIALRTQERNPIRLDVGYLP
                     QGDLMVSAKGLSNGLYSGGIIMTTFAGMGVATGITLTAVGCSTDHPTLCRAGIITAIP
                     SAVGLYLGISWMRKALPKWNVERAQPYVAGSNVGVAGRF"
     gene            4881..5168
                     /gene="groES"
                     /locus_tag="VFV99_12190"
     CDS             4881..5168
                     /gene="groES"
                     /locus_tag="VFV99_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5060117.1"
                     /translation="MKIRPLHDRILVKRLEEETKTSGGLFIPDSAKEKPIQAKVIAVG
                     AGKRDKDGKIIALEVKAGDKVLFSKYSGTEVKIDGEEHLIMREDDLLAVLD"
     gene            5220..6851
                     /gene="groL"
                     /locus_tag="VFV99_12195"
     CDS             5220..6851
                     /gene="groL"
                     /locus_tag="VFV99_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830443.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5060118.1"
                     /translation="MAAKEIIFSSSARSEIAKGLNLLANAVKVTLGPRGRNVVIEKSW
                     GSPTVTKDGVTVAKEVEVENKFQNMGAQMVKEVASKTSDVAGDGTTTATVLAQAIYNE
                     GAKLVAAGLNPMDLKRGIDAAVASVVEQIKSMSTPTKGKEDIAQVGTISANGDTEIGE
                     MIAEAMKKVGKEGVITVEEAKTLHSELDVVEGMQFDRGYLSPYFVTDSERMEVVLNDV
                     YILIHEKKISNMKELLPVLEQVAKQGKPLLIVAEDVDGEALATLVVNKLRGTLQVCAV
                     KAPGFGDRRKAMLQDIAALTGGEAVTEDLGLKLENLTLQDLGRAKKIQVDKDNTTVIE
                     GAGKKDAIEARVKTIRREVEETTSDYDREKLQERLAKLVGGVAVIRVGAATEVEMKEK
                     KARVEDAMYATRAAVEEGIVPGGGVALVRTIPALDKLTFDDDRRYGVNIVRRALEEPL
                     RQIAGNAGTDGSIVVAKVKEGKGAFGFNAATLQYEDLVKAGVIDPTKVVRTALQNAAS
                     VAGLMLTTETLIAEKPKKEEKAGGGHEGHGHDFDH"
BASE COUNT         1340 a         2251 c         2255 g         1083 t
ORIGIN      
        1 ataacccgca gacgatcacg atcacatcgg acgcgtacct gggcagcccg caggccgtcg
       61 aagtgcgctg caagatcgca gatacgacgt tgcagatcat gccgacgatg ttcgacttcg
      121 gtgaagtccg cgtcgctgcg actgcaccgt cgatcccgat cacgctgacg aatccggcga
      181 gctcggccgg cgccgcaatg atcaccgcgt tcgatctgcg cctccacaag aacgggctga
      241 cgctgacgaa gccggccgta ccgttctcga tcgccgccgg cacggtcgca aacgcatcgc
      301 tctcggtctc gaccacggag gacaccgatc tcactggcga gtatctcgac atcaccgtcg
      361 acggctcgac gctgagcttc ccggtcaccg gcaaggtcgt gcacccccac gcatacgtga
      421 cgccgacgaa catgctcgat ctcggcaccg cctgcgtcgg tacgcagatc agcggcgacg
      481 tcgtgatgac gaacgatggc accgcgacgc tgttcgtgat gccgcccgtg atgacgccga
      541 gcttcgtcgc ggccgcaccg gccacgcccg gcccgttgcc gccgggcatg tcgcgcacgg
      601 cgaccgtcac gccggcgatg agcgcgatgg gaccgatcga aggcacgctg tcgtggaccg
      661 aggacacgcc gatgacgcac gaggtcggcg tgaagctcga ctacgtcgcg accggcacgg
      721 cgctgtcgcc gcgcggcctc gacttcggca tcgtgcccgt cgacgacgat ccgctcaccg
      781 agtacaccca gcacctcacg ctgcagaact gtgatcaggc gccgacctcg atcaagatcg
      841 agtcgctgaa gacgaaggcc ggcacgctcg gcgcctggca cctcgtgccg aatgtcggct
      901 acacgaagca gctcggcgcg aaagagacgc aagcggtcca ggtgacgttc gacccgccgg
      961 cgcgcggcaa gtacgaagcg gatatgacgg tgcagacgtc gagcggcaag caggtcgtgc
     1021 acctgatcgg cgacgcgacc ggccgcgact tcgacaacac cagcttctac gcgtgcgcgt
     1081 gcaacggacc cggcgcgccg tcacgcgggt ggccgatcgt gctcgcggtc gccatcgtta
     1141 tcgtccgccg ccgccgcccc ggaccttctt cacctcggtg aagaacagat ccacttcgtc
     1201 cggcttcgtg tagaagtgcg gcgcgatccg aatgccggcg ctcggccggt gatcgcagaa
     1261 gaacttgcgg cgattgagct cgcggcacac gtccgcggag ccgacgaagt cgaacacgac
     1321 cgaaccgccg cggacctctg ccggccgcgg gctgttgagc ttgaggccga gctcgtcgac
     1381 ccaatcgatg catttctggg tcatcacgag gctcttctcg cggatcttgc gcacgccgat
     1441 ctcgccaatg atcgactgcc ctgcgcgcga ctgatacagc gccgccaccg ctggcgtgcc
     1501 gcccatgtag cgccacacat tgtcggcgta cgactgctcc ggcatcgtga acgcgaacgg
     1561 cgactcgttg ccgaaccaac cagtgacgcg cggcgcgaac tgctcgatca gatccttgcg
     1621 cacgtagagc catgccgcgc cgggaccgcc gcacacgtac ttgaccgagc cgccgcacgc
     1681 gaacgacacg ccgagatcga cgacatcgat cggcagcgtg ccgatcgact ggtacgcgtc
     1741 gaggatcacg tgcgcgccga cttccttcgc gcgcgcgacg atcttcttga cgtcctggat
     1801 ctgcgccgtc gcgaacacga cgtgctgcag cggcacgagc agcgtgcgct cgtcgatcgc
     1861 ggcgcagatc gcgtccgcat ccatcgtgcc gtccttcgct ggcacaacga cgcactcggc
     1921 gccgcgccgc ttctccgcct gccagacata cgagaccgtc gggaacatca gcgactcgta
     1981 gacgaccttg ttgcgcgggg ccgtgaactc gaagcacgac gcgagtgtcg ccatgatctg
     2041 cgacacgttg tggtgcatga tcacggtgcc gtcgggcgcc gagatgatct tcgcgatgcg
     2101 cgcagcggcg cgatcgacct cgggcaacca gtcgctccac gcggtgatcg acttggtgcg
     2161 ccatagctcg aagaattcgc cgaggtcctc tttggtctgg gcgggaacgc agccgagcga
     2221 gtgcgagatg aagtggacgc actcttcgag cgccggaaag tgcttgcggt actcgactag
     2281 gctctcggcg acgggatcgg ccatgcgcag tggcttatca tgaagcgcga tggtcgacga
     2341 gacggtgaag gattggatca cccaacagat ggcgctgctc atcaagcgca cagggctcga
     2401 cagctacgtc gcagcgccgc tcgtcgagca aaccgatcaa tggtttccgg acgaatggca
     2461 tcctgacgat gcgggggtcg cccggctcgg caagcgcgtg ttcgagcacg cgggcctgcc
     2521 cgatgtcgag atcgcgaccg agctgacgaa gaacaccggg agcaaaggca tcgtcgcgac
     2581 acacgtcgac gcgaaggtcg tccgcctcga tgtcgacccc gagttcctca ccgatccgct
     2641 cacgatcatc tcccacctga tttcgctcgc cgccgtcgtg tttcgcgtgc gccacgagct
     2701 cgaggatcca gatcacgaca ccgagcggcg cctcatcgat ctgacgacga tctatctcgg
     2761 cttcggcatt ctcaccacga acgcggcgta tcgctaccgt gcaagcggcg agctgcaagg
     2821 cagcaccgcg atcacgcggt ggtcgcacga cgtgcaaggt tcgctgcccg cagacgcgat
     2881 ggcgtacgca ctcgcacagc agctcgtcgc gcgcaatgca tcgcccgcgg agctgcgcaa
     2941 cgtgaagcgt cagctcgaga cgaatcaggc cgaggtgttc gagaccacgt gtcgtcagct
     3001 caagaccgac tacgtcgtgc tcgcgctcga tctgccggca cgcaacaagt ggcctgcgcc
     3061 gcggcaacca cctgccccgc cgacgagcac ggcgctgcgg ctgtggaaga agcccgcgtc
     3121 gaatctgccg gtcgcgacgg tgaagagcac cggctacctc ggcttcaaca gcggccgctc
     3181 ggtgctgcgc gtgccacgca atcggcgctg ggaagccggc atgatcggct tgttcgtctc
     3241 gatggtgccg acgatcgcgc tcgccgtgaa aggcttcggc ggtgcggcga tgggcgcgat
     3301 ggccgccaca tggctgctca gcgtgatgct cggcaacaag tgggtgcgcg agacgtgctc
     3361 cgatccgaag tgcgagatga agctcgagcg cggcgttcaa acgtgcccgc gctgcggcgg
     3421 aacgatcgcc ggcaccgtgc agaaaggcga gaaccgcctc gaagccgagg agcggttgca
     3481 gctcaatcaa ggtgactacg acatggacgt cggcgatccc gccgaaagcg gcaccgcgtt
     3541 gcccagcgcg cgtgtcgaac gcgagcgcgc gaccagcgaa ggcagcacga gtacgatcgt
     3601 ctagccgccg taggagagcg cggcgtcgcc caccggaatc cccgcgtcgg gctgcgctgt
     3661 cggtgcgacc ggcgcgatcg gcgcgaccgg cgccactggc gcgacaggag ccatgatacc
     3721 ggcgtcgagc ggaagtccgg gcggctcgtc tgcgttctgc ggcgcgacga tgacgtcgcc
     3781 ctcgtcatcg ttcgtcagct cgtgggcgga tgtgtcagcg aacatcgtga cgagcccggt
     3841 gatgccgccg agcgcgacgc cggcgatcgc cccgatcgcg ccgcacgtct ttaccttctc
     3901 gaccgagatc tcgcaggcac caaagccgat cgtgcctgca acgatgccga ccgtgatccc
     3961 cggatgcttc tgcgcaaacc cgcagccacc gagcacgaac agcatcgcga agagcgctgc
     4021 tccgcgttgt cggcgagacc ttccgctgac ttccatcctg tcggcggatc taccacattg
     4081 cagcgcccga accgcttcgg cgaagatgag gtcgtgcgcc gtccgtacct aagtgagtag
     4141 cctcatgaag tacggagcct tgttcgcagt gcttgcgctg tctggacccg cgttcgcgca
     4201 gcctggtgcg ccggagccaa acgcgtactg gccgccgccg gaccaggcgc agcagccgca
     4261 gccgcaaccg cagcagaatg cagcgacgcg cgcgacgttc gtatcgagcg gcgaggagaa
     4321 atgggatgtg tgggtcgatc agcagcccgc atgcgcgacg ccgtgcacga tcggcgtgat
     4381 gccgctgcag tacatcgcgc tacgtacgca ggagcgcaat ccgatccggc tcgacgttgg
     4441 ctacttgccg cagggtgatc tgatggtgag cgcgaagggg ctgtcgaacg ggctctactc
     4501 gggcggcatc atcatgacga cattcgcggg catgggcgtg gcgaccggca tcacgctgac
     4561 cgccgtcggt tgctcgaccg atcacccgac gctgtgccgc gccggaatca tcacagcgat
     4621 tccaagcgcg gtgggcctgt acctcggcat ctcctggatg cgtaaagcgc tgccgaaatg
     4681 gaatgtcgag cgcgctcagc cgtacgttgc cggcagtaat gtgggcgtcg ccggccggtt
     4741 ctgacctgat attgcgccag ctaccacaaa gtaattgctg gcctaatcag aattttgtta
     4801 gcactcaccg ggaggggttg ccaacgacct ggagcgtcgt tataggtggc gaccaagaac
     4861 ccaatttctg gagtgaggca atgaagatcc gtccgctgca cgatcgaatt ctggtcaagc
     4921 gtctcgaaga ggagaccaag acctctggtg gtctgttcat tccggactcg gcgaaggaaa
     4981 agccgatcca agccaaggtg atcgccgtcg gcgcgggtaa gcgcgacaag gacggcaaga
     5041 tcatcgcgct cgaggtgaag gccggcgaca aggtgctgtt cagcaagtac agcggcaccg
     5101 aagtgaagat cgacggcgaa gagcacctca tcatgcgcga ggacgatctc ctcgcggttc
     5161 tcgactaatc aacatcgctc gcgccattgc gcgcgagctc acgcaacgag gtttttccaa
     5221 tggctgccaa agaaatcatt ttttcgtcga gcgcccgcag cgagatcgcc aagggcctca
     5281 accttctcgc caacgcggtg aaggtcaccc tcggcccgcg tggccgtaac gttgtcatcg
     5341 agaagtcgtg gggctcgccc acggtgacca aggacggcgt cacggtcgcg aaggaagtcg
     5401 aagtcgagaa caagttccag aacatgggcg cgcagatggt gaaggaggtc gcttccaaga
     5461 cctctgacgt cgctggtgac ggcacgacca ccgcgaccgt gctcgcgcag gcgatctaca
     5521 acgagggcgc gaagctcgtc gccgccggcc tgaacccgat ggatctcaag cgcggtatcg
     5581 acgcggctgt cgcgtcggtc gtcgagcaga tcaagtcgat gtcgacgccc accaagggca
     5641 aggaagacat cgcgcaggtc ggcacgatca gcgcgaacgg tgacaccgag atcggcgaga
     5701 tgatcgccga ggcgatgaag aaggtcggca aggaaggcgt catcaccgtc gaagaggcga
     5761 agacgctgca cagcgagctc gacgtcgtcg agggtatgca gttcgaccgc ggctacctgt
     5821 cgccgtactt cgtgacggac agcgagcgca tggaagtcgt gctcaacgac gtctacatcc
     5881 tcatccacga gaagaagatc tcgaacatga aggagctcct gccggttctc gagcaggtgg
     5941 cgaagcaggg caagccgctc ctcatcgtcg cggaagacgt cgatggtgaa gcgctcgcga
     6001 cgctcgtcgt caacaagctc cgcggcacgc tgcaggtctg cgccgtcaag gcgccgggct
     6061 tcggcgaccg tcgcaaggcg atgctgcagg acatcgcggc gctcaccggt ggcgaagcgg
     6121 tcaccgagga tctcggcctc aagctcgaga acctgacgct gcaggacctc ggccgcgcga
     6181 agaagatcca ggtcgacaag gacaacacca cggtcatcga gggcgccggt aagaaggacg
     6241 ccatcgaggc acgggtgaag acgatccgtc gcgaggtcga ggaaaccact agcgactacg
     6301 atcgcgagaa gctccaggag cgcctcgcca agctcgtcgg cggcgtcgcg gtcatccgcg
     6361 tcggcgcggc gaccgaggtc gagatgaagg agaagaaggc gcgcgtcgag gacgcgatgt
     6421 acgcgactcg cgcggctgtc gaagagggca ttgtccccgg cggcggtgtt gcgctcgttc
     6481 gcaccatccc ggcgctcgac aagctgacgt tcgacgacga ccgccgctac ggcgtcaaca
     6541 tcgtccgccg cgcactcgag gagccgctgc gccagatcgc tggcaacgca ggcaccgacg
     6601 gctcgatcgt cgtcgcgaag gtcaaagagg gcaagggcgc gttcggcttc aacgccgcga
     6661 cgctccagta tgaagacctc gtcaaggccg gcgtcatcga cccgaccaag gtcgtccgca
     6721 cggcgctgca gaacgccgcc tcggtcgcgg gcctcatgct cacgacggag acgctgatcg
     6781 ccgagaagcc gaagaaggaa gagaaggcgg gcggtggcca cgagggtcac ggccacgact
     6841 tcgatcactg attcgaatct cgttccgtaa gtgaagggcc ggtcgcaaga ccggctcttt
     6901 gcgttttcgg gaattcacga aggttgaac
//
LOCUS       DASUFX010000310         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_602142,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000310 DASUFX010000000
VERSION     DASUFX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602142"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VFV99_12200"
     CDS             complement(<1..1205)
                     /locus_tag="VFV99_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5060119.1"
                     /translation="MSALHRKLRRDLWRLKGQVATIALVLACGIMAMIMLRSTWQSLL
                     GARDAYYEQYRFADVFAHLERAPESVGARLEQLPGIAIVHTRITEDVMVPLAGEPDPV
                     TGKIVSLPESGIPPLNQLYLRAGRLPIGDDEAVVLEQFASAHALQPGDRLPVVINGRL
                     RSIVVVGITLSPEYVLAMSGRELAPDNRRFVVLWMLRDAVAPAFRMEGAFNDVVARLE
                     PGASVPAALAAIDRELARYGGLHAVARDKQMSNYALTNELGVLRTLALIIPAIFLAVA
                     AFLVNVVVSRLVFLERTQIAVLKAIGYTNRSVALHYLGLVALIVSISAIGGVLAGVKT
                     GQWMTNLYADFYRFPTRVHHVAPMLVALTLGIGLAAAVTGALSAVRRIARMPPAQAMR
                     PPAPLDYRKT"
     gene            complement(1202..1861)
                     /locus_tag="VFV99_12205"
     CDS             complement(1202..1861)
                     /locus_tag="VFV99_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5060120.1"
                     /translation="MGEIDVIALRGVDLDLVDGELMVLLGVSGSGKSTLLNIIGGLDV
                     CTTGKVLYRGFDLTAADDAELTEYRRRHVGFVFQFYNLLPSLTARENVELVTDIADDP
                     MDAAEALELVGLGHRLDHFPAQLSGGEQQRVAVARAVAKRPDVLLCDEPTGALDFDTG
                     IKVLAALQRVNRELGTTTAIITHNAPIAAIAERVVTLADGKIARIEVPAVRKSAEELR
                     W"
     gene            2063..>2940
                     /locus_tag="VFV99_12210"
     CDS             2063..>2940
                     /locus_tag="VFV99_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060121.1"
                     /translation="MRRVALVLVLSGCFHDAAKPTTSRGLVPGVRFAIAVGKTVQIVR
                     TTDNGLVVERTTPVPDAVSRIVWVGPDPAVWIDMTVGEQGPGFPPFNYDEDPKPPGAH
                     QNEMGLVTASGYVKFKPIAWPAAKRPDGQFSEDLSEVTEPNAALVASKDALWEKRCRW
                     YGGPDGGWCTYEYARRFPEPTVFVAEVDETRALAFPKIPPSATLAVGHKTRPRDPNNT
                     DADSFENNQEVMTCKRAGGEAITYPPAGALDYNVLKDIDWLSTEPPIFVSSEEIPDYS
                     GMGRTPPPSEKIWEGCT"
BASE COUNT          533 a          968 c          982 g          457 t
ORIGIN      
        1 gtcttgcggt agtcgagtgg cgccggcggt cgcatcgcct gcgccggtgg catccgcgcg
       61 atccgccgca ccgccgagag cgccccggtt actgccgccg cgagaccgat gccgagcgtc
      121 agcgccacga gcattggagc cacgtggtgc acgcgggtag ggaagcgata gaagtccgcg
      181 tacaggttcg tcatccactg gcccgtcttc acgccggcga ggacgccgcc gatcgcggag
      241 atcgatacga tcaacgcgac gagcccgagg tagtgcagcg cgacgctgcg attcgtgtag
      301 ccgattgcct tgagcaccgc gatctgcgtg cgctcgagga acacgagccg cgacacgacc
      361 acgttgacga gaaatgctgc gaccgcgagg aagatcgccg ggatgatcag cgcgagcgtg
      421 cgcaacacgc cgagctcgtt cgtgagcgcg tagttcgaca tctgcttgtc gcgcgcgacc
      481 gcgtgcaacc cgccgtaccg cgcgagctcg cgatcgatgg cagcgagcgc cgcggggacg
      541 gacgcaccgg gttcgagccg agccaccacg tcgttgaacg cgccttccat gcggaacgct
      601 ggagcgaccg cgtcgcgcag catccagagc acgacaaatc gccggttgtc gggtgccagc
      661 tcgcgtcccg acatcgcaag gacgtactcg ggagacagcg tgatgccgac gacaacgatc
      721 gaccgcaacc ggccattgat cacgaccggc agtcgatcgc cgggttgcag cgcgtgcgcg
      781 gacgcgaatt gctcgagcac gaccgcttcg tcgtcgccga tcggcaaccg gccggcacgc
      841 aagtagagct ggttgagcgg cggtataccg ctctctggca gcgacacgat cttaccggtg
      901 accggatccg gctcgccggc gagcggcacc atcacgtcct cggtgatgcg cgtgtgaacg
      961 atcgcgatgc cgggtagctg ctcgagccgc gcaccgaccg attcgggcgc acgctcgagg
     1021 tgagcgaaca cgtcggcgaa ccggtactgc tcgtagtacg cgtcgcgtgc acccaagagc
     1081 gactgccacg tactgcgcag catgatcatc gccatgattc cgcaggcgag gacgagcgcg
     1141 atcgtcgcca cctgaccctt caggcgccaa aggtcgcgtc gcagcttgcg atgcagcgcg
     1201 ctcaccagcg cagctcctcg gcggacttgc ggaccgccgg cacttcgatg cgcgcgatct
     1261 tgccgtccgc gagcgtgacg acacgctctg cgatagccgc gatcggtgcg ttgtgcgtga
     1321 tgatcgcagt cgtcgtgccg agctcgcgat tgacgcgctg gagcgcggcg agaaccttga
     1381 tgccggtatc gaagtcgagc gcgccggtcg gttcgtcgca cagcagcacg tcgggacgct
     1441 tcgcgaccgc tcgtgcgacg gcaacgcgct gctgttcgcc accggacaac tgcgccggaa
     1501 agtgatcgag gcgatggcct agcccgacga gctcgagagc ctcggcggcg tccatcggat
     1561 cgtcggcgat atcggtgacg agctctacgt tctcgcgcgc cgtcaggctc ggtagcaaat
     1621 tgtagaactg gaacacgaac ccgacgtggc gccggcgata ctcggtgagc tcggcatcat
     1681 cagcagccgt gagatcgaag ccgcggtaga gcaccttgcc ggtggtgcag acatcgaggc
     1741 cgccgatgat gttgagcaag gtcgacttgc cgctgcctga gacacccagc aagaccatca
     1801 gctcgccgtc gacgagatcg aggtcgacgc cgcgcagcgc gatcacatcg atctcaccca
     1861 tccgatagct cttggtcagc ccgcgcgcgg aaatcaccag ttcgcgggag tcagcgatgc
     1921 gcggcgtcgc cataacactg gatgagtgca gcgtctgtgc ccgcacatcg tgcacgtctc
     1981 agcgattccc gcgaacgcgc tgcggcaccc ctgcgggccc ccgcaaacgg ttcgcgaacg
     2041 cgcaatcgcg taggatgccc ctatgcgccg tgtcgccctc gtgctggttc tgagcggttg
     2101 ttttcacgat gcagcgaagc cgacgacctc gcgcggcttg gtgcccgggg tgcgctttgc
     2161 gatcgcggtc ggcaagaccg tgcagatcgt tcgcacgacc gacaacggcc tcgtcgtcga
     2221 gcgcacgaca ccggtgcccg acgcggtcag ccgcatcgtg tgggtcggtc cggatcccgc
     2281 ggtgtggatc gacatgacgg tcggtgagca agggcccggc tttccgccgt tcaattacga
     2341 cgaggatccg aaaccgcccg gtgctcacca gaacgagatg ggcctcgtca ccgcgagtgg
     2401 ctacgtcaag ttcaagccga tcgcctggcc ggcggcgaag cgcccggatg gtcagttcag
     2461 cgaggacctc agcgaggtca ccgagccgaa tgcagcgctc gtcgcgagca aggacgcgct
     2521 ctgggagaag cgatgtcgct ggtacggcgg ccccgacggt ggttggtgca cgtacgagta
     2581 cgcgcgccga tttcccgagc cgacggtgtt tgtcgccgag gtcgatgaga ctcgcgcgct
     2641 cgcgtttccc aagatcccac cgtccgcgac gctcgcggtc ggccacaaga cgcggccgcg
     2701 tgacccgaac aacacggacg cggacagctt cgagaacaac caggaagtga tgacgtgcaa
     2761 gcgtgcaggc ggcgaagcga tcacgtatcc gcctgccggc gcgctcgatt acaacgtcct
     2821 caaggacatc gattggctct cgaccgagcc gccgatcttc gtcagcagcg aagagatccc
     2881 ggactactcc ggcatgggcc gaacgccgcc gcccagcgag aagatctggg agggttgcac
//
LOCUS       DASUFX010000311        16027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1298056, whole genome shotgun sequence.
ACCESSION   DASUFX010000311 DASUFX010000000
VERSION     DASUFX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16027
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298056"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2279
                     /locus_tag="VFV99_12215"
     CDS             <1..2279
                     /locus_tag="VFV99_12215"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX5060122.1"
                     /translation="RLERSAIQRVRIALSKIDASKLGASGKLDGELAISAIDAGGKVD
                     VKGVVTDVGTLDSQLALAPGPGGEIAAHGTLQLGGVDPVDVNATLALPIHPFDPRGWK
                     QLGRATLREATVEAKRIAFDPDLMKRFGVDSPWRGWAAVNIKVGPEARTSEFVVDVHE
                     LRDGPLKKPVDLHIAGGSDANGPHITATAKADKVEIALAGKSPLSVEAMLAGNARTAP
                     IEATLTVPKTAARELALLVGRDDVLGGLISGEVTVGGTIDKPTARAVLAVDNLAVSGG
                     VAAKPPTLEKLELDARWLGVERGFELEVTGHEPGGRLLKISARGKPSEPEGIVASIEA
                     GNFDIAPFVAFAPPTSMAIGTRGQISGVLKLKGIDPNTGDVKGRLVISNARVPLAPEL
                     GNLRSGTVEISVLKKEVVALIDGKLGRGTVKGKATVRLTGSMPTAAELSLTLRKISLI
                     GEVQPQIDADVSGYLVRTKTKWTGTIKVANGNVYVPPESGNELLVTATPSDIVFIDAA
                     PVLVKPKRRPPTKPWLIATIDIGRTKILVDDQNFRFEGAASGQLKLELGDGIGLDGSL
                     STERGRVDVLGRGYQLDHGIIDFDGTLDPRLDIQMMHSFTTLALTVEIGGRSSQPDLR
                     LSSDSGAYSQGQLLGFLAGGTPSDDPSQQSSDALASGSLTLLSSRLGRKLNRVPFIKF
                     DTINYEAKTASSSRAIRFGKRLGEHTYLNLRKRFEPRLDENSQEATVEHELRKDVLIE
                     ATGGERGAGADLLWRKRW"
     gene            2295..3140
                     /locus_tag="VFV99_12220"
     CDS             2295..3140
                     /locus_tag="VFV99_12220"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5060123.1"
                     /translation="MVETVLDYLREPLVDLGGTHVTTLTLFTALVIVFAARIIAAIVG
                     RSIERVLEVRKVEAGLRHAVGKMTRYTVIVLGVFVALGTVGVDTSAILGAGAVLLVGI
                     GFGLQKLAENFISGLLVLIERPVRKGDFIDAGGVLGTVEDIGLRATRVMSRDGVTVIL
                     PNSGLISGTVINYGQPTPERRLQVDIGVAYGTDLDLAVRVLKEIATAEPMVLENPAPE
                     VRHYGFGDSSIQLALIVWIGEAREDLIAGSKLRFAIDKAFRKYKIEIPFPQRQVHMIP
                     PKHVA"
     gene            3137..4333
                     /locus_tag="VFV99_12225"
     CDS             3137..4333
                     /locus_tag="VFV99_12225"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5060124.1"
                     /translation="MKVSASQARAIALAAQGFAAPRPSRVDAKQLRALVERLGVVQID
                     SVNVLVRSHYLPAFSRLGSYDPMHFDRHAHRAPRALFEYWGHEASLLPVALQPLFRWR
                     MERAREHAWGRMSRIAKKRPTFVQSVLALVGEKGPISASEMELGGKKSRKGWWEWSDA
                     KTAIEWLFWSGQVTSARRRGFERLYDLPDRVIPAKVLAQPTPTEPDAHRALVERAARA
                     LGIASEADLRDYYRLPLAGARTAVAELVEAGTLEHVTVEGWAKPGYLHRAAQVPKRID
                     PDRPALLSPFDSLVWFRDRTHRLFGMKYRIEIYVPAPKRVHGYYVLPFLLGDELVARV
                     DLKADRATSTLRVQASHAEPGVAKPRVASALAGELSRMAEWLGLARVEIVPKGDLAAA
                     LAKATR"
     gene            complement(4323..4676)
                     /locus_tag="VFV99_12230"
     CDS             complement(4323..4676)
                     /locus_tag="VFV99_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060125.1"
                     /translation="MGTLSKRPLRDFSDDVKDRIENVRTSVGSKLESMRGRAEDASRH
                     EGAVTKAIEKLTAALPSTTWLLFAGASLIGAIAFRAARRNHASLFIGQWVPTFLILGL
                     YNKLVKVEGSERASG"
     gene            4883..6664
                     /locus_tag="VFV99_12235"
     CDS             4883..6664
                     /locus_tag="VFV99_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060126.1"
                     /translation="MLRIALLLGVLAATPAFADDDVPFDPTIDECAAPDSGCLPDAAE
                     PTDESLAAEEAAMVEDVAPIDWTVAQDRVACPDGATARDGQCIAGADVTADVSGGCTA
                     GHTPGIVLGIAVLALLVYARRRRVLILLAACSLDGQSWDDAVEGGPTGDAASYVDVFS
                     ADRGDSDGAQYLLANQAIADGNEQPVAQFALLRAHDGVPVFRTPGSCGDRLATSGDEL
                     LGYARADAGDGTAALVELAAPDGCAFTYETDPETVDILVAQGYRAVGTVAHVWPPGIG
                     DTPPVEPTTPDDMTLEATAPAACPNVTKHSPLQLLYASPGKDYTWRFLRGCPGEVVVG
                     EKRESGPVGRMRSAEAQAAGGRTGFVLDRHGDKLRELLLRDNGVERTAAYLRHKMQLG
                     YDYFVIDEITAAADFRDGATLNRRLRKLLLRMPARTIIPYISIDLTQYSTGFSSMQGR
                     RLLLRAFKRRARSVALEVYLHTAQVRAGYAPSAFRRAADRLALSVRGLQYGGGINRRA
                     ITVIGTSIHGGTSNLAQYSYLDQPSHDLASLSRQVNAIRHGSKRLRQQHGVGWYFVFA
                     GDMEPRPGGYTYEALIRRMRLLGLRFK"
     gene            6760..8139
                     /locus_tag="VFV99_12240"
     CDS             6760..8139
                     /locus_tag="VFV99_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5060127.1"
                     /translation="MRKRRAARETVASELHPWSECDEAHAKKVARIAAQLRAHDGKKP
                     VSLRKKAPPHQVPKGGDLRRRDAKIDVSDLTTIIDIDPVKRICVAESGVMFCDLVEAT
                     LRYGLVPIVVPELKTITIGGAVSGCSIESMSYRYGGFHDTCIEYEVVTARGDVLICKP
                     DNHHSLVFQMVHGSFGTLGILTRLKFRLVPAKPYVRVDYETYPTLVDYHAAIERHFKA
                     EDVDFMDGIMHTPQELVLCVGRFVDSAPFTNAYDWTKVYYQSTRKLAFDYLETPDYFF
                     RYDRGVTNVHPKSYVGRLLFGKLMDSGTTLRIADKLNFMFKSDRPTVILDVFLPFSQA
                     MAFMEWYEREVGHFPLWCVPYKRVHDYEWLDDSFYAGMTDQLFLDLAIYGMAQPEGKN
                     IHRMIEEKLRELGGVKTLISHNYYSKDEFWSIWNKRNYDTVKAITDPQNLFRDLYSKT
                     CRAAMGLPG"
     gene            8644..9288
                     /locus_tag="VFV99_12245"
     CDS             8644..9288
                     /locus_tag="VFV99_12245"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX5060128.1"
                     /translation="MKAITRALLIGLLFVGSAYADDPTKTTDTTKTNKDTTAKKEKLT
                     PAELQVMAHYKDVNMTEIDLGKVAKKQGGTAAVKSYGEMMVKDHGAMDKQLKDLAKMT
                     KQTIPAEKMTTDAEKQAQAEMKKDVAALKKLKGADFDRAYIQMMIDGHEREVSNIDSK
                     MAEVNNPELKTLLTDLKPQLQHHEDAAKELQKNNAQAMNQPHTNQPQAKQQPRK"
     gene            complement(9289..9642)
                     /locus_tag="VFV99_12250"
     CDS             complement(9289..9642)
                     /locus_tag="VFV99_12250"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5060129.1"
                     /translation="MVDSRATLRYRIDLPAQLEVGGAALSARIRNLSLGGVRLAGPPL
                     PVGARCRLTFLSPHGDSFDLWCITRWTTTDGCGLEFEALQPIDTNQLARMIGSSSRVT
                     ERIERIPIESIQRLG"
     gene            9949..11514
                     /locus_tag="VFV99_12255"
     CDS             9949..11514
                     /locus_tag="VFV99_12255"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEX5060130.1"
                     /translation="MNGKFTDAQECDKACSVGVGCTACEIGKATCNGNTATVCDESGN
                     GFVTVECDPVQGMTCDPNADGGCVGACAPKSLGTSYIGCDYFPTVTGNDTGTMFDFAV
                     AIANTTAGDAMVTIEGGALSATQIVTVPSNRVVVQTLPWQAELKLCNTPQTDGCFGTV
                     QSAALAMKGAYRLRSTSPVTVYQFSPLQYEKAGAFSYTNDASLLLPTNVWRTRYFAAA
                     WQALSGASYPSELAVTASHDNTTVTIQAKATTLAGQGAPGFMQGVGQQVTLNAGDVIE
                     ISTLSGDLTGSLVDADKPVQVISGHFCANVPDGTGYCDHLEESMFPVDTLGTHYIVNA
                     PAVTTIPTGKVEAVRIIATGGNTTLQYDPPQPSAPTTIANAGDFIEISGNANSFQIIA
                     DQKVLVVQYMEGQDAGGNTGDPAMTLAVPVEQFRTQYLFHAPTNYESNYVDVTAPVGA
                     QVMLDGAPISFAPIGTSGYALSRVYPLNAGPNNDGNHSIQGNMPFGITVYGYGQYTSY
                     WYPGGLDLNTIIQ"
     gene            11677..12534
                     /locus_tag="VFV99_12260"
     CDS             11677..12534
                     /locus_tag="VFV99_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060131.1"
                     /translation="MLKYLVASLLLTACTADVDILSEDEGATSGASVTSVPRSLTGCN
                     GHASTTIPSDGRYVITTFGGPGDHQPMSCGGYADGTGWYAASRQRYGCGSHVKVQANG
                     KCVVLKTDDYGPDVCVENAAHMPIIDVSPRASRELFGVSGAGWSDHLVVTVEEVPAST
                     PLGVCAPSDGGGGGGGGGGGTAMSCASATLDRDVAAGECVQAASDGVMYQCTNGAWVA
                     KSGTTGCTATYAWCSSPTLGKSVAPRACVQSAASGTWFQCNGQDWAKPVTVSTKEGPI
                     GACSEWHPL"
     gene            12637..13860
                     /locus_tag="VFV99_12265"
     CDS             12637..13860
                     /locus_tag="VFV99_12265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5060132.1"
                     /translation="MFRLDEQTQMVATMVRQWCQSQLAPKIPAFEHGEEQPFELMKKM
                     AKTFGFDQMLGGSVQKRIAKLRAGEVSDPDTTLAKGVGNPMMMHVVVKELSRISPGFA
                     MGFGVSLGLVGGAIVAKGTPDQIEKWGLPLATLQQIGSWCLTEPNAGSDAFGSMRTTA
                     RPEGSDYILKGSKTFITNGPGADVFLVYAKIDRGEPAAGQGVATFIVERNTPGVTVGP
                     PFKKMGMRDSPTSEVFFDDVRLGTEHLLGGKEKGRGGRSDTKDSLGNERSGVPAMAWG
                     IIERCYDASLQYVRDRVQFGRPIGAFQAVQLKITDMFLKLRTVENVVYRTAWMQQNGV
                     KDMPFVNASKAMCAQMAVDAALTSIQIHGGYGYMEELGLEKLARDAKLLELGAGTTDI
                     NLLACARELIGLHDA"
     gene            13850..14341
                     /locus_tag="VFV99_12270"
     CDS             13850..14341
                     /locus_tag="VFV99_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060133.1"
                     /translation="MMRSLLLVLALAACGKSDKPKPEAHDNVIAPRALEGLAFDLPDQ
                     WTKTYDTDHDAWNIASADAATKVLIERADERYVASPDAYMHHVSQQWPGKLVTIESRK
                     NVKGGFAMTLGIFTGEKDPNPQHATVVVRELGSRWYRCISEGADSEEIREQVLALCRS
                     VRL"
     gene            complement(14338..14880)
                     /locus_tag="VFV99_12275"
     CDS             complement(14338..14880)
                     /locus_tag="VFV99_12275"
                     /inference="COORDINATES: protein motif:HMM:NF025791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-4 family immunity protein"
                     /protein_id="HEX5060134.1"
                     /translation="MSSRRQVLERLPQEVRLEGWVGPTMFTSEVRDGVVITLEELDRR
                     FLGHEHARSLVIGTIRETEAAETDDGPSGGPYRGAVMSGANGWAYAAFCIDPRTENIV
                     LIDFDDVRPTTRFVNSSLALFLASLNAFLEAWPRLAGSDDDAAVAWSAGVCRELEAID
                     PAAMSDGDDFWPVSLETYTF"
     gene            complement(15469..>16027)
                     /locus_tag="VFV99_12280"
     CDS             complement(15469..>16027)
                     /locus_tag="VFV99_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060135.1"
                     /translation="SNLILICDGHHKLLHEGIVTITGAAPDQLVFTRDGRQLVDPRSP
                     AAIGQAMTLRETAAVTETVSPKPRSRFADAVRYEQAKQALKGLGFKATLAKQLLEEAL
                     THVGQDAEVSTIVEKVLAMNRATTTADVIEDQSKGMLAKRALVQSGYAPALAEAAVEA
                     ALTRVGSDKPLETLIMEAFRRCCEG"
BASE COUNT         2932 a         5303 c         5183 g         2609 t
ORIGIN      
        1 agcgcctcga gcgctccgcg atccaacgcg tgcgcatcgc gctgtcgaag atcgatgcga
       61 gcaagctcgg tgcgagcggc aagctcgacg gcgagctcgc gatcagtgcg atcgatgccg
      121 gcggcaaggt cgatgtcaaa ggcgtggtca ccgacgtcgg cacgctcgat tcgcagctcg
      181 cgctcgcgcc aggcccgggc ggtgaaatcg cggcgcacgg cacacttcag ctcggcggcg
      241 tcgatccggt cgacgtcaac gcgacgctcg cgctgccgat tcacccgttt gacccgcgcg
      301 gctggaagca gctcggtcgc gcgacgctac gcgaagcaac tgtcgaggcg aagcgcatcg
      361 cattcgatcc cgacctgatg aagcggttcg gggtcgacag cccgtggcgc ggctgggcgg
      421 cggtcaacat caaggtcggc ccggaggccc gcacgagcga attcgtcgtc gacgtccacg
      481 agctgcgcga tggtccgctc aagaaaccgg tcgatctgca catcgctggc ggcagcgacg
      541 cgaacggccc gcacatcact gccaccgcga aggccgacaa ggtcgaaatc gcactcgccg
      601 gcaagtcacc gctgtcggtc gaggcgatgc tcgccggcaa cgcacgcacc gcgccgatcg
      661 aagcgacgct cacggtgccg aagaccgccg cgcgcgagct cgcgctgctc gtcggtcgcg
      721 acgacgtgct cgggggtctg atttcgggcg aggtcaccgt cggcggcacg atcgacaaac
      781 cgaccgcgcg cgccgtgctc gccgtcgaca acctcgcggt ctctggcggc gtggcggcca
      841 agccaccgac gctcgagaaa ctcgagctcg acgcccgctg gctcggtgtc gaacgtggtt
      901 tcgagctcga ggtcaccggt cacgagcccg gcggccgtct gctcaagatc agcgcgcgag
      961 gcaagccgag tgagcccgaa ggcatcgtcg cgtcgatcga agccggcaac ttcgacatcg
     1021 cgccgttcgt cgcgtttgcg ccgccgacga gcatggcgat tggcacgcgc gggcagatca
     1081 gcggcgtgct gaagctcaaa ggtatcgatc cgaacaccgg cgacgtgaag ggccggctcg
     1141 tcatcagtaa cgcacgcgtc ccgctcgcgc cggagctcgg caacctgagg tccggcaccg
     1201 tcgaaatcag tgtgctcaag aaggaggtcg tcgcgttgat cgacggcaag ctcggcaggg
     1261 gcacggtgaa aggtaaagca acggtgcgcc tgaccggcag catgccgacc gccgccgagc
     1321 tgagcctcac gctgcgcaag atctcgctca tcggcgaggt ccaaccgcag atcgacgccg
     1381 acgtcagcgg ctacctcgtg cgcacgaaga cgaagtggac cggaacgatc aaagtcgcca
     1441 acggcaacgt ctacgtgcca cccgagagcg gcaacgagct actcgtcacc gccacaccca
     1501 gcgacatcgt gttcatcgat gcggcgccgg tactcgtcaa gccgaagcgc agaccgccga
     1561 cgaagccctg gctgatcgcg acgatcgaca tcggccgcac gaagatcctg gtcgacgatc
     1621 agaactttcg cttcgagggc gccgcgagcg gccagctgaa gctcgagctc ggtgatggca
     1681 tcggcctcga tggttcgctc tcgaccgagc gcggcagagt cgacgtgctc ggtcgcggct
     1741 atcagctcga ccacggcatt atcgacttcg acgggacgct cgatccgcgg ctcgacatcc
     1801 agatgatgca cagcttcacg acgctggcgc tgaccgtcga gatcggcggc cgctcgagcc
     1861 agcccgatct acgcctgtcg agcgactcgg gcgcctacag ccagggccag ctgctcgggt
     1921 tcctcgccgg tggcacgccg agcgacgacc cgagccagca atcgagcgat gcgctcgcga
     1981 gcggcagcct cacgctgctg tcatcgcggc tcggccgcaa gctcaacagg gtgccgttca
     2041 tcaagttcga cacgatcaac tacgaggcga agaccgcgtc ctcgagccgc gcgatcaggt
     2101 tcggcaagcg cctcggcgag cacacgtatc tcaacctgcg caagcgcttc gagccgcggc
     2161 tcgatgaaaa ctcgcaggaa gcgacggtcg agcacgagct gcgcaaggac gtgctgatcg
     2221 aggcgaccgg cggcgagcgc ggagccggcg ccgacctgtt gtggcgcaag cgctggtgat
     2281 aacgtcgcta cgacgtggtg gaaaccgtcc tcgattacct gcgtgagccg ctggtggacc
     2341 tcggcggcac acacgtcacg acgctgacgc tgttcaccgc gctcgtgatc gtgttcgccg
     2401 cacgaatcat cgccgcgatc gtcggtcgca gcatcgagcg cgtactcgag gtgcgcaagg
     2461 tcgaggccgg cctgcgtcac gcagtcggca agatgacgcg ctacaccgtg atcgtgctcg
     2521 gcgtgttcgt cgcgctcggc accgtcggcg tcgataccag cgcgatcctc ggagccggcg
     2581 ccgtgctgct cgtcggcatt ggcttcggtc tccagaagct cgccgagaac ttcatctccg
     2641 gtctgctcgt gttgatcgag cggcccgttc gcaaaggtga cttcatcgat gccggcggcg
     2701 tgctgggcac cgtcgaggac atcggcctgc gcgcgacacg cgtgatgtcg cgcgacggcg
     2761 tgaccgtcat cctgccgaac tcggggttga tctcgggcac ggtgatcaac tacggccaac
     2821 cgacgccaga gcggcgcctg caggtggaca tcggcgtcgc gtacggcact gatctcgacc
     2881 tcgcggtgcg cgtgctgaag gagatcgcaa ccgccgaacc gatggtgctc gagaacccgg
     2941 cgcccgaagt gcgccactac gggtttggcg acagctcgat ccagctcgcg ctgatcgtct
     3001 ggatcggtga ggcacgcgaa gacctgatcg ccggcagcaa gctgcggttc gcgatcgaca
     3061 aggcattccg caagtacaag atcgagatcc cgttcccgca gcgccaggtc cacatgatcc
     3121 cgccgaagca cgtggcgtga aggtctccgc atcacaggct cgcgcgatcg cgctcgccgc
     3181 gcagggcttc gccgctccga ggccgtctcg cgtcgacgcc aaacagctgc gcgcgctggt
     3241 cgagcggctc ggcgtcgtcc agatcgactc cgtcaacgtg ctcgtgcgct cgcactacct
     3301 gccggcgttc agccggctcg gcagctacga cccgatgcac ttcgatcgcc acgcgcatcg
     3361 cgcaccgcgc gcgttgttcg agtactgggg tcatgaagcg tcgctattgc cggtggcgct
     3421 gcaaccgctg tttcgctggc gcatggagcg cgcacgcgag catgcgtggg gacgcatgag
     3481 ccgcatcgcg aagaagcggc cgacgttcgt ccagagcgtg cttgctctgg tcggcgagaa
     3541 ggggccgatc agcgcgagcg agatggagct cggcggcaag aagagtcgca aaggctggtg
     3601 ggagtggtcc gacgcgaaga ccgcgatcga atggctgttc tggagcggcc aggtcacgag
     3661 cgcgcgccgg cgcggcttcg agcggctcta cgatctgccc gaccgcgtga ttcccgcgaa
     3721 ggtgctcgcg cagccgacac cgaccgagcc cgacgcgcat cgcgcactcg tcgaacgtgc
     3781 ggcgcgagcg ctcggcatcg cgagcgaagc cgacctccgc gactactacc ggctgccgct
     3841 cgccggagcg cgcaccgccg ttgcggagct cgtcgaggcc ggcacgctcg agcacgtcac
     3901 cgtcgagggc tgggcgaagc ccggctatct ccaccgcgcg gcacaagtgc caaagcgcat
     3961 cgatcccgat cgccctgctc tgctctcgcc gttcgattcg ctcgtgtggt ttcgcgatcg
     4021 cacgcatcgc ctgttcggca tgaagtaccg catcgagatc tatgtgccgg cgcccaagcg
     4081 cgtgcacggc tactacgtgc tgccgttcct gctcggcgat gagctcgtcg cacgcgtcga
     4141 tctcaaagcc gatcgtgcga caagcacgct gcgcgtacag gcctcgcacg ccgagcccgg
     4201 cgtggcgaaa ccgcgcgtcg catcggcgtt ggccggggag ctatcccgga tggccgagtg
     4261 gctcggtctc gcgcgcgtcg agatcgtgcc gaaaggcgat ctcgcagctg cgttagcaaa
     4321 agctacccgc tagcgcgttc gctgccctcg accttcacca gcttgttgta gaggccgagg
     4381 atcagaaatg tcggcaccca ctggccgatg aagagcgaag cgtgatttcg ccgcgccgcc
     4441 ctgaacgcga tcgcaccgat cagcgacgca ccggcaaaca ggagccacgt cgtcgacggc
     4501 aacgccgccg tcagcttctc gatcgccttc gtgacggcgc cctcatgacg gctcgcgtcc
     4561 tcggcgcggc cgcgcatgct ttcgagcttg ctgccgacgc tcgtgcgcac gttctcgatc
     4621 cgatctttga cgtcgtcact gaaatctctc agtggacgtt tgctgagcgt tcccatgttt
     4681 cctccttggg tctacgggca agggctgcag cgcgtgtgcc acggcacggc gaactgaagc
     4741 ccgatcgctt catcaaaccg cgcacatcgc cacgcacacg aacgtgcgca gtcgagggga
     4801 aagcccgtca cctgcagatg cgcaagcacg cagattcgca atgcgcagcg cgtggcacag
     4861 ctcgcgcaat tgccgccgcg ccatgcttcg catcgcgctt ctcctcggtg tgctcgcagc
     4921 aacgcctgcg ttcgccgacg atgacgttcc gttcgacccc accatcgacg agtgtgccgc
     4981 acccgacagc ggctgcttac ccgacgcggc ggagccaacc gacgaatcac tcgctgccga
     5041 agaagcggcg atggtcgaag acgtcgctcc gatcgactgg actgtcgcgc aagaccgggt
     5101 cgcctgcccc gacggtgcga ccgcacggga tggtcagtgc atcgccggcg ccgacgtcac
     5161 cgccgatgtc agcggcggct gcacggccgg ccacacgccc ggcatcgtgc tcggcatcgc
     5221 cgtgctcgca ttgctcgtct atgcgcggcg ccgccgcgtg ctgatcctgc tcgctgcgtg
     5281 ctcgctcgac ggccaaagct gggacgacgc cgtcgagggt gggccgaccg gcgacgcggc
     5341 gtcctatgtc gacgtgttct cggccgaccg cggcgatagc gacggcgcgc agtaccttct
     5401 cgcgaaccaa gcgatcgccg atggcaacga gcaacccgtc gcgcagttcg cactgttgcg
     5461 cgcacacgac ggagttcccg tgtttcgcac gcctggctcg tgtggcgatc gcctcgcgac
     5521 gagtggcgac gagctcctcg gctatgcccg cgcggacgcc ggcgatggca ctgctgcgct
     5581 cgtcgagctc gcagcgcccg atggctgcgc attcacctac gagaccgatc cagaaaccgt
     5641 cgacatactc gtcgcacaag gctatcgcgc cgtcggcact gtcgcgcacg tgtggccgcc
     5701 cggcatcggc gacacaccac cggtcgagcc gacgacgccg gacgacatga cgctcgaagc
     5761 gaccgccccc gccgcgtgcc ccaacgtcac caagcactcg cccctgcaac tactctacgc
     5821 gagccccggc aaggactaca cgtggcggtt cttgcgcggc tgtcccggcg aggtcgtcgt
     5881 tggcgagaag cgcgaatccg gcccggtggg acggatgcgc agcgccgagg cacaagcggc
     5941 cggcgggcgc accggcttcg tgctcgatcg ccacggtgac aagcttcgcg agctgctcct
     6001 ccgcgataac ggcgtcgagc gcacggcggc ctatctgcgg cacaagatgc agctcggcta
     6061 cgactacttc gtgatcgacg agatcaccgc cgctgccgat ttccgcgacg gcgcgacgct
     6121 caatcgccga ctgcgcaagc tgctgctccg catgcccgcg cgcacgatca ttccgtacat
     6181 ctcgatcgat ctcacgcagt actcgactgg attctcgtcg atgcaaggcc gccgcttgct
     6241 gctgcgcgcg ttcaagcgcc gcgcacgctc ggtcgcactc gaggtctacc tccacaccgc
     6301 ccaggttcga gccggctacg caccgagcgc attccgccgc gccgccgatc gcctcgcact
     6361 ctcggtccgc ggcctccagt acggtggcgg catcaaccgc cgcgcgatca ccgtgatcgg
     6421 aacgtcgatc cacggcggca cgtcgaacct cgcgcagtac agctacctcg atcagccgtc
     6481 acacgacctc gcgtcgctgt cgcgccaggt caacgcgatc cgtcacggca gcaagcggct
     6541 gcgccagcag cacggtgtcg gctggtactt cgtgtttgcc ggcgacatgg agccacgacc
     6601 gggcggctat acgtacgaag cgctgattcg ccgcatgcgt ctgctcgggc tgcgcttcaa
     6661 gtaggtacgc gcgttgctca ctcgcgggtg atgtcacgcg tccgcctgct aactgctgca
     6721 agtctcgtcg gaagcgcgct cgcgataggt cgctcgatcg tgcgcaagcg ccgcgctgcg
     6781 cgcgaaaccg tcgcgagtga gctgcatccg tggtcggagt gcgacgaagc gcatgcaaag
     6841 aaggtggcac gcatcgccgc gcaactacgg gcgcatgacg gcaagaagcc ggtgtcgctg
     6901 cgtaagaaag cgccgccgca tcaggtgccg aagggcgggg atctgcggcg acgagacgcg
     6961 aagatcgacg tctcggacct caccacgatc atcgatatcg acccggtgaa gcggatctgt
     7021 gtcgccgaat ccggcgtgat gttctgcgac ctcgtcgaag cgacgcttcg ctacggcctc
     7081 gttccgatcg tcgtccccga gctcaagacc atcacgatcg gcggcgcggt ctcgggttgc
     7141 tcgatcgagt cgatgtccta tcgctacggc ggcttccacg acacgtgcat cgaatacgag
     7201 gtcgtcaccg cgaggggcga cgtcttgatc tgcaagcccg ataaccacca ctcgctcgtg
     7261 ttccagatgg tccacggctc gttcgggacg ctcggcatcc tgacccggct gaagttccgg
     7321 ctcgtaccgg cgaaaccgta cgtgcgcgtc gattacgaga cgtacccgac gctggtcgac
     7381 taccacgcgg cgatcgagcg ccacttcaaa gccgaagacg tcgacttcat ggacggcatc
     7441 atgcacacgc cgcaggagct cgtgctgtgc gtcggccgct tcgtcgacag cgcgccgttc
     7501 acgaacgcct acgactggac gaaggtctat taccagagca cgcgcaagct cgcgttcgac
     7561 tatctcgaga cgcccgacta cttcttccgc tacgaccgcg gcgtcacgaa cgtgcacccg
     7621 aagtcgtacg tcgggcggct cttgttcggc aagctgatgg attcgggcac gacgctcagg
     7681 atcgcggaca agctcaactt catgttcaag agcgatcgcc cgacggtgat cctcgacgtg
     7741 ttcctgccgt tctcgcaggc gatggcattc atggagtggt acgagcgcga ggtcggtcac
     7801 ttcccgctgt ggtgcgtgcc gtacaagcgc gtccacgact acgagtggct cgacgacagc
     7861 ttctacgcgg gcatgacgga ccagctgttc ctcgatctcg cgatctacgg aatggcgcag
     7921 cccgagggca agaacatcca ccgcatgatc gaggagaagc tgcgcgagct cggcggcgtg
     7981 aagacgctga tctcgcacaa ctactattcg aaggacgagt tctggtcgat ctggaacaag
     8041 cgcaactacg acacggtgaa agcgatcacc gatccgcaga atttgttccg cgacctgtac
     8101 tcgaagacct gtcgcgcggc gatgggtctt cccggctagc gtcgcctgat tccacccagg
     8161 cgctctgcgc ggatgtcgcg gtcatgcaaa ggccgcgacg ttcggatcgc aagctcagtc
     8221 gataccgcgg cagcaggaat cgtcgtcgtc gtcgatccag cgacgcacag cagcattgcg
     8281 ctcatcggcc accttgcgcg ctcgtacatc gtcgggacct gcactgttac ggggtgagct
     8341 ctctcgggtt ggacggcgtt gaaggcgctg ctgttgctcg gtcatgtgtg gtgtggactg
     8401 caatgggtgt gcgcaaccgg cgagtggcgt tcgggcagtt tagggacgag agtccgtgcg
     8461 tcgacgcgca tcgcgcgtac gggggtaccc catactcttg caacgcgatt cagcggccaa
     8521 cttattcgcc gtagttgccc gcgcgttggc gctcgtcgtg gcgattcgta gcgatccgac
     8581 tgccgcgata cgcagaaatg acgtgtggcc tggcggtctt cgctttgcac ggcgcagagc
     8641 gccatgaagg caatcacgag agccctgttg atcggtctgc tgttcgtcgg atccgcctat
     8701 gcggacgacc cgacaaagac gacggacacg acgaagacga acaaggacac gacggcgaag
     8761 aaggaaaagc tcacgccggc agagctgcag gtgatggcgc actacaaaga cgtcaacatg
     8821 acggagatcg acctcggcaa ggttgccaag aagcaaggcg gcacggccgc ggtgaagtcc
     8881 tatggcgaga tgatggtcaa ggaccacggc gcgatggaca agcagctcaa agacctcgcg
     8941 aagatgacga agcagacgat ccccgccgag aaaatgacga ccgacgccga gaagcaggcg
     9001 caggccgaga tgaagaagga cgtcgccgcg ctcaagaagc tcaaaggcgc ggacttcgat
     9061 cgcgcgtaca tccagatgat gatcgatggt cacgagcgcg aggtgtcgaa catcgactcg
     9121 aagatggccg aggtgaacaa ccccgagctc aagaccctgc tcaccgatct gaagccgcag
     9181 ctgcagcatc acgaggacgc cgcgaaggaa ctgcagaaga acaacgcaca ggcgatgaac
     9241 cagccgcaca cgaaccagcc gcaggcgaag cagcagccga ggaagtgatc agccgaggcg
     9301 ctggatcgat tcgatcggaa tgcgttcgat ccgttcggtg acgcgcgacg agctaccgat
     9361 catgcgcgcg agctgattgg tgtcgatggg ctgcagggct tcgaactcga gcccgcagcc
     9421 atcggtcgtc gtccagcgcg tgatgcacca cagatcgaac gagtcgccgt gcggcgacag
     9481 aaacgtgagc cggcaccgcg caccaaccgg caagggcggc cccgcgaggc gaacgccgcc
     9541 aagcgacagg ttccggatac gcgcggagag cgccgcaccg ccaacctcga gctgcgccgg
     9601 caggtcgatc cggtaccgca gcgtggcgcg cgagtcgacc atggcctccc taggatgcgt
     9661 ggctcaactc gacgaatcca cgattctttt cgtccctcac ggtcacaccc tgtctccatc
     9721 gagaaaggtg cgtatcggtc agtacacccc agggccgctg taacaaaatg ctgcggcact
     9781 tagaagtctc ttcacaaacc ctggggctct gggttctagt gcgttgcgat gagagggcta
     9841 gcgatactcg cgatcgttat tggtgcgtct tgcggaccgg ggcagcgaca agacggcagt
     9901 tgcaatgccg gcgagacata ctgtgatggg ctcacctttc agacctgcgt gaacggcaag
     9961 ttcaccgacg cgcaggaatg tgacaaggcg tgctcggttg gtgtcggttg cacggcgtgt
    10021 gagatcggca aggcaacctg caacggcaac accgcgaccg tgtgcgacga gagcggcaac
    10081 ggcttcgtca ccgtcgagtg cgatccggtg caaggcatga cgtgcgatcc gaacgccgat
    10141 ggcggctgcg tcggtgcgtg cgcgccgaag agcctcggca cgagctacat cggctgcgat
    10201 tactttccga cggtcaccgg caacgacacg ggtacgatgt tcgacttcgc ggtcgcgatc
    10261 gcgaacacga ccgcaggcga tgcgatggtc acgatcgaag gcggtgcgct cagcgcgacg
    10321 cagatcgtca cggttccaag caatcgcgtc gtcgtgcaga cgctgccgtg gcaagcggag
    10381 ctcaagctct gcaacacacc gcagaccgat ggctgcttcg gtacggtgca gagcgcggcg
    10441 ctcgcaatga agggcgccta ccgcctacgc tcgacgtcgc cggttacggt ctatcagttc
    10501 agcccgctgc aatacgagaa ggccggcgcg ttctcataca cgaacgacgc gtcgctgctg
    10561 ctgccgacga acgtgtggcg cacgcggtac ttcgcagccg cgtggcaggc gctctccggc
    10621 gcgtcgtatc cgagcgagct cgcggtcacc gcgtcgcacg acaacacgac cgtcacgatc
    10681 caggcgaagg cgaccacatt ggcgggccaa ggtgcgcccg ggttcatgca aggcgtcggt
    10741 cagcaggtca cgctcaacgc aggtgacgtc atcgagatct cgacgctgag cggcgacctc
    10801 acgggttccc tcgtcgacgc cgacaagccc gtccaggtca tcagcggcca tttctgcgcg
    10861 aacgttcccg acggcaccgg ttactgcgat cacctcgaag agtcgatgtt cccggtcgac
    10921 acgctcggca cgcactacat cgtcaacgcg ccggcggtca cgacgatccc cactgggaag
    10981 gtcgaggccg ttcgtatcat cgcgacgggc gggaacacga cattgcaata cgatccgccg
    11041 cagcccagcg cgccaacgac gatcgcgaac gcgggcgact tcatcgagat cagcggcaac
    11101 gccaactcgt tccagatcat cgccgatcag aaggtgctgg tcgtgcagta catggaaggc
    11161 caggacgcgg gtggcaatac cggcgatccc gcgatgacgc tcgccgtgcc cgtcgagcag
    11221 ttccgcaccc agtatctgtt ccacgcaccg acgaactacg agtcgaacta cgtcgacgtc
    11281 acggcgccgg tcggtgctca agtgatgctc gatggtgcgc cgatctcgtt cgcgccgatc
    11341 gggacaagcg gctacgcgct atcccgcgtc tacccgctca atgccggccc gaacaacgac
    11401 ggcaaccact cgatccaggg caacatgccg ttcgggatca cggtctacgg ctacggccaa
    11461 tacacgagct actggtatcc cggcggcttg gacctgaaca cgatcattca gtgagtggga
    11521 gtggtcccac cgtcgctgac taccggttgc actgaaatgt gcggcggcta acggcgatca
    11581 cggccgtttc gtgcacgcgt cgcgcaacgc cttgcacgcc gagatccagc atcgcgattt
    11641 gccgaagtcg gcatatcgac tgcacatcgc ggcgccgtgc tgaaatacct cgtcgcttcg
    11701 ctcttgctta ccgcgtgcac cgccgacgtc gacatcctca gtgaagacga aggcgccacc
    11761 tccggcgcca gcgtcacgtc cgtcccacgc agtctgaccg gctgcaacgg gcacgcgtcg
    11821 acgacgatcc cctccgacgg ccgctacgtc atcacgacgt tcggtggccc gggcgatcac
    11881 caaccgatgt cgtgcggtgg ctacgcggac ggcaccggct ggtatgcggc atcgcggcag
    11941 cgatacggct gtggctcgca cgtcaaagtg caggcgaacg gaaagtgcgt tgtgctcaaa
    12001 accgacgact atggtccgga cgtctgcgtg gagaacgcgg cccacatgcc gatcatcgat
    12061 gtctcgccgc gtgcgtcgcg cgagctgttc ggcgtctcgg gtgcaggctg gagcgatcac
    12121 ctggtcgtga cggtcgagga agtgccggca tcgacgccac tcggtgtctg cgcgccgtcc
    12181 gatggcggcg gtggcggtgg cggcggtggt ggcggcacgg cgatgtcgtg tgcgtcggcg
    12241 acgctcgacc gcgacgtcgc agctggcgag tgcgtgcagg ccgcgagcga cggcgtcatg
    12301 taccagtgca cgaacggcgc gtgggtcgcg aagtcgggca cgaccggctg cacggcgacg
    12361 tacgcctggt gcagctcgcc gacgctcggc aagagtgtgg cgcctcgcgc ctgcgtgcag
    12421 tcggccgcgt cggggacctg gttccagtgt aacggccagg actgggcgaa gcccgtcacg
    12481 gtctcgacga aggaaggccc gattggcgcc tgctccgagt ggcacccact atgaagcccg
    12541 acgaaggaag cgtattcgcg ctccggctcc cggccgaccg cgatccggcc gacgacagcg
    12601 cggtatttgg gcctgaatct cgtctacgat gcgggcatgt tccgcctcga cgagcagact
    12661 cagatggtgg ccacgatggt ccggcaatgg tgtcagtcgc agctggcacc taagattccg
    12721 gcgttcgaac acggcgagga gcagccgttc gaactgatga agaaaatggc caagacgttc
    12781 ggcttcgacc agatgctggg cggctcggtg caaaagcgca tcgcgaagct ccgcgcgggc
    12841 gaagtcagcg accccgacac gacgctcgcc aagggcgtcg gcaatccgat gatgatgcac
    12901 gtcgtcgtca aggagctgtc gcgcatctcg cctggcttcg cgatgggctt cggcgtctcg
    12961 ctcggcctcg tcggcggcgc gatcgtcgcg aagggcacgc ccgaccagat cgagaagtgg
    13021 ggcctgccgc tcgcgacgct gcagcagatt ggctcgtggt gtctgaccga gccgaatgcg
    13081 ggctcggatg cgttcggatc gatgcgcacg accgcgcgtc ccgaaggcag cgactacatc
    13141 ctgaagggca gcaagacgtt catcacgaac ggcccgggcg cggatgtgtt cctcgtctac
    13201 gcgaagatcg atcgcggcga gccggccgct ggccagggcg tcgcgacgtt catcgtcgag
    13261 cgcaacacgc cgggcgtcac ggtcggcccc ccgttcaaga agatgggcat gcgcgactcg
    13321 ccgacgagcg aggtgttctt cgacgacgtg cgcctcggca ccgagcacct gctcggcggc
    13381 aaggagaaag gccgcggcgg gcgaagcgat accaaggaca gcctcggcaa cgagcgctcg
    13441 ggtgtgcccg cgatggcgtg gggcatcatc gagcgctgct acgacgcgtc gctgcagtac
    13501 gtgcgcgacc gcgtccagtt cggccggcca atcggcgcgt tccaggccgt gcagctgaag
    13561 atcacggaca tgttcctcaa gctgcgcacc gtcgagaacg tcgtgtaccg cacggcgtgg
    13621 atgcagcaga acggcgtcaa ggacatgccg ttcgtcaacg cgtcgaaggc gatgtgcgcg
    13681 cagatggcgg tcgacgcggc gctcacgtcg atccagatcc acggtggcta tggctacatg
    13741 gaagagctcg gcctcgagaa gctcgcgcgt gacgcgaagc tgctcgagct cggtgccggc
    13801 acgaccgaca tcaacttgct cgcctgcgcg cgcgaattga tcggcctgca tgatgcgtag
    13861 cctgctgctg gtgctcgcgc tcgccgcgtg cggcaagagc gacaagccca agcccgaggc
    13921 gcacgacaac gtcatcgcac cgcgcgcgct cgaggggctg gcgttcgacc tgcccgacca
    13981 gtggacgaag acgtacgaca ccgaccacga cgcgtggaac atcgcctctg cggacgcagc
    14041 gacgaaggtg ctgatcgagc gcgccgacga gcgctacgtc gcgagcccgg atgcgtacat
    14101 gcaccacgtc tcgcagcagt ggccgggcaa gctcgtcacg atcgagtcac gcaagaacgt
    14161 gaagggcggg ttcgcgatga cgctcgggat cttcacgggc gagaaggatc cgaatccgca
    14221 gcacgcgacc gtcgtcgtgc gcgagctggg gagccgctgg taccgctgca tctcggaggg
    14281 cgcggatagc gaagagattc gcgagcaggt gctcgcgctc tgccggtcgg tgcggctcta
    14341 gaacgtataa gtctcgagcg acacgggcca gaagtcatcg ccgtcactca tcgcggccgg
    14401 atcgatcgcc tctagctctc tgcaaacccc tgcggaccaa gccacagcgg catcatcatc
    14461 cgaacccgcg agccgcggcc acgcttcgag gaacgcgttc aggctcgcaa ggaacaacgc
    14521 caagctggaa ttgacgaagc gggtcgttgg tcgcacatca tcgaaatcga taagcacgat
    14581 gttctcggtg cgtgggtcga tgcaaaacgc cgcataggcc cacccgttgg ctccactcat
    14641 caccgcaccc cgatatggcc cgccggaggg tccatcgtca gtttctgcgg cttccgtttc
    14701 gcggatcgtg ccgatcacca gagaccgggc gtgctcgtgc cccaggaaac gacgatcgag
    14761 ctcctcgagc gtgatgacca caccatcgcg aacttccgag gtgaacatcg tcggcccgac
    14821 ccagccctcg aggcggacct cttgcggtaa acgttcgagg acctgacgcc ggctgctcac
    14881 gatgaacaca ttctactcgc tcgccgcgaa gtctgaacgc aaggctctgc tcgcgcggac
    14941 atcgggcgct gtgcagtcac gcagcctcgc gggagacgaa tgcactctgc agtcgtgcag
    15001 cgtccgggcg tggggcggtc tgctcgtacc accgcgcgaa acaagtttcg cgctgccgac
    15061 agcgcgcgcg gtctgcggag cacgatcctc ccggatgggt ctcttagatc gacgtcgcga
    15121 gaccgtcgcg cagaacacga ccgcttcgcc acaacgatca tcggcgcgga tctctaggtc
    15181 tgttgctttg atcgtctgct cgtcctgacc cacgtgggtc agcgatgctt tgcgcatcgg
    15241 ccaccgagcg cctccttcca gcccgacgca ccactccgcc tgctcgcgca gcgaggccgc
    15301 gctatcgcgt cgacgcagat ctcggcgatt catcgatgaa ggacggccga ctcagacgtc
    15361 gtggcgacgc ctcttacatg gtcagcgttt ccgacagcga cgcaaccgtt ccatgatcag
    15421 cgcctccacc ggctcgtcct gacccacgtg gggcagcgag ttttcgcttc acccctcgca
    15481 acagcgacga aacgcttcca tgatcagggt ctcaagcggc ttatctgaac ccacgcgggt
    15541 cagtgcagct tcgaccgccg cttccgcaag cgccggcgca tatccggact gaacgagcgc
    15601 acgtttcgcg agcatcccct tactttggtc ctcaataacg tccgcggtcg tcgttgcgcg
    15661 gttcatcgcg agcaccttct cgacgatcgt gctgacctct gcatcctgac ccacgtgggt
    15721 cagcgcctct tcgagcagct gcttcgccag cgttgccttg aagcccagcc cctttagcgc
    15781 ctgcttggct tgttcgtagc ggacggcgtc agcgaaacgt gagcgcggct tcggtgagac
    15841 ggtctcggtc accgcggcgg tttctcgcag ggtcatcgcc tggccgatcg cagccggcga
    15901 acgcggatcg acgagttgtc taccgtcgcg cgtaaagaca agttggtcag gagctgcgcc
    15961 ggtgatcgtc acgatgcctt catgcagcag cttatggtgg ccatcgcaga tcaggatcag
    16021 attcgac
//
LOCUS       DASUFX010000312         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_326518,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000312 DASUFX010000000
VERSION     DASUFX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326518"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..539)
                     /locus_tag="VFV99_12285"
     CDS             complement(174..539)
                     /locus_tag="VFV99_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5060136.1"
                     /translation="MAARDIVLDYLRAIESHDLDRVAAFLHADVQTIEHPNKLNPKGA
                     TYDRAAMRAAGERGAALMASERYEVRALTCEGDRVVAQIAWTGTLKDGRSMRAQICSA
                     IELKDGLIWRQEQYDCFEV"
     gene            complement(600..1541)
                     /locus_tag="VFV99_12290"
     CDS             complement(600..1541)
                     /locus_tag="VFV99_12290"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEX5060137.1"
                     /translation="MCLAIAMFALAGCRQIFGLEPPATGDAAQADAPASCSDNQLSPG
                     ETDVDCGGICPACGTGASCTQHSDCKTGVCAGTKCLLAKTCLEIRNAGQLTSGIYPIA
                     PNAMTFDAYCDQTTEGGGWTLVMKLSSASLALRFDAPQWTTAATLFEDDLDPNSAPQG
                     RDAKLVAYNEVAGTEMRLRWLDPDHSFAYTPPSATTALGVFMGGEDLVVGKETDQCNG
                     QLLSMSPSFLANGMRHATGHQFYGVNGQDNANGTNSHMRWGFGSNDEQTNPWLPHQGA
                     GTDDTSLTWDSQTDCTECSCYGNEYVPSETSANMWIR"
     gene            complement(1689..2735)
                     /locus_tag="VFV99_12295"
     CDS             complement(1689..2735)
                     /locus_tag="VFV99_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5060138.1"
                     /translation="MTTATLIEGDGIGPEIASATVRAVEAAGGKITWERAAAGANAVE
                     KFKDPLPQATIDSMKKNGLALKGPLATPTGGGYRSVNVTLRQQFDLYANVRPVQTIPG
                     VPSRYTNVNLVIVRENTEDLYAGVEHYVDPRRTAAESIAIITKFGSERIVVYAFEYAR
                     RHHRKKVTLVHKANILKMSNGLFLDTGREVAKRYPDIQFDDMIVDATAMKLVIAPERF
                     DVIVTMNLFGDILSDLTAGLVGGLGVAPAANIGDGGCAMFEAVHGTAPDIAGKGIANP
                     TGLMLSAAMLLDHVQQGAAASRLRAGIKAALFNAETRTGDLGGRANTVGFTDAVIRSM
                     ELAPDNSGVIRAMD"
     gene            complement(2735..3064)
                     /locus_tag="VFV99_12300"
     CDS             complement(2735..3064)
                     /locus_tag="VFV99_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5060139.1"
                     /translation="MSSWHPVAKVGALKPGDVIAVEAEGVPMILGLDGDRYFAMQRQC
                     VHRGGDLSDGIIARGHVVCANHGWRFSTATGRHDQASDVCLVVYAVRINGENIEVDPI
                     PRTLRGH"
BASE COUNT          525 a         1091 c         1026 g          586 t
ORIGIN      
        1 aggcggacac cattcggcag ctcggcaggc ccgagcggta gcagcacgcc gggaaagcag
       61 cccgcgagca gcgcggcgcc ggcgagcaaa tacctcatcc ctcacgagac accacgcgaa
      121 cacgtcagtt gcccagcgaa atggggtctg accggtctga ccccatttcc gggctacacc
      181 tcgaagcagt cgtactgctc ttggcgccag atcaggccgt ccttcagctc gatcgcactg
      241 cagatctggg cgcgcatcga ccgcccgtcc ttcagcgtgc cggtccacgc gatctgtgcg
      301 acgacccgat caccctcgca ggtcagcgcg cgcacctcgt agcgctcgct cgccatcagc
      361 gccgcgccgc gctcgccggc cgctcgcatc gccgcccggt cgtacgtcgc gcccttcggg
      421 ttgagcttgt tcggatgctc gatggtctga acgtcggcgt ggaggaacgc cgccacgcga
      481 tcgaggtcgt gcgattcgat cgcgcgcaga tagtcgagga cgatgtcgcg ggcagccata
      541 gccgtgcgat gaccgtaacg cacgattcga ttcgcatgtt ctgactgccc gccgctgcgt
      601 cagcgaatcc acatgttcgc gctggtctcg ctcggcacgt actcgttgcc gtagcacgag
      661 cactcggtgc agtcggtctg cgagtcccac gtgaggctcg tatcgtcggt ccccgcgcct
      721 tggtgtggga gccacggatt ggtctgctcg tcgttactgc cgaatcccca ccgcatgtgg
      781 ctgttggtcc cgttcgcgtt gtcctggccg ttgacgccgt agaactggtg gccggtcgcg
      841 tgacgcatgc cgttcgccaa gaagctcggc gacatcgaga gcagctggcc gttgcactgg
      901 tcggtctcct tgccgacgac caggtcctcg ccgcccatga atacgccgag cgcggtcgtc
      961 gcgctgggcg gcgtgtacgc gaacgagtga tcggggtcga gccaacgcaa acgcatttcg
     1021 gtgccagcaa cctcgttgta cgcgaccagc tttgcgtcgc gcccctgcgg cgcgctgttg
     1081 gggtcgagat cgtcctcgaa caacgtcgcc gctgtcgtcc actgcggagc atcgaacctc
     1141 agcgccaggc tcgcgctcga cagcttcatg acgagcgtcc agccgccacc ttcggtcgtc
     1201 tgatcgcagt acgcgtcgaa cgtcatcgcg ttcggtgcga tcggatagat gccagacgtg
     1261 agctgacccg cgttgcggat ctcgaggcag gtcttcgcga gcaggcattt ggttccggca
     1321 cacacgccgg tcttgcagtc gctgtgttgc gtgcagctgg cgccggttcc gcacgcggga
     1381 cagatgccgc cgcaatcgac atcggtctcg cctggcgaca gctggttatc gctgcacgac
     1441 gccggtgcgt cggcttgtgc agcgtcgccg gtcgcgggcg gttcgaggcc gaagatctgt
     1501 cggcagcccg ctagcgcgaa catcgcgatc gccaggcaca cccgagcgca cacactctag
     1561 actcccacgt cgcggcaacc gacgggtgag aaactcgtgc tgcgccgatc gcgcacgaac
     1621 gtgcacactc agcgtggcgg tttcgacgtg tcgaattcgt ctgcgtgcgt cgagatcgtc
     1681 gtcgacgatc aatccatcgc gcggatgacg ccgctgttgt cgggcgcgag ctccatcgag
     1741 cgaatgacag catcggtgaa gccgaccgtg ttcgcgcggc cgccgaggtc acccgtgcgg
     1801 gtctcggcgt tgaacagcgc ggccttgatg ccggcgcgca gccggctcgc cgcggcgcct
     1861 tgctgcacgt gatcgagcag catcgcggcc gatagcatga ggccggtcgg attcgcgatg
     1921 cccttgccgg cgatatccgg cgccgtgccg tgcaccgcct cgaacatcgc acagccgccg
     1981 tcgccgatgt tcgccgccgg tgcgacgccg aggccaccga cgaggcccgc cgtgaggtcc
     2041 gacaggatgt cgccgaacag gttcatcgtg acgatcacgt cgaagcgctc gggcgcgatc
     2101 acgagcttca tcgctgtcgc gtcgacaatc atgtcatcga actggatatc gggataacgc
     2161 ttcgcgacct cgcgaccggt gtcgaggaac aggccgttgc tcatcttcag gatgttcgcc
     2221 ttgtggacga gcgtgacctt cttacggtga tgccggcgcg cgtactcgaa cgcgtagacg
     2281 acgatgcgct cgctgccgaa tttcgtgatg atcgcgatcg actcggccgc ggtgcgccgc
     2341 ggatcgacgt agtgctcgac gcccgcgtaa aggtcctcgg tgttctcgcg cacgatcacg
     2401 agattgacgt tcgtgtaccg cgacggcacg cccggaatcg tctgcaccgg ccgcacgttc
     2461 gcgtagagat cgaactgctg gcgcagcgtc acgttgaccg accgatagcc accaccagtc
     2521 ggcgtcgcga gcgggccctt gagcgcgagc ccgttcttct tcatcgaatc gatcgtcgcc
     2581 tggggcagtg gatccttgaa cttctcgacg gcgttcgcgc cggccgctgc acgttcccac
     2641 gtgatcttgc cgcccgctgc ttcgaccgca cgcacggtcg cagacgcgat ttcagggcca
     2701 atgccgtcgc cttcgatcag agtcgccgtt gtcatcagtg tcctcgcagg gtccgcggaa
     2761 tgggatcgac ctcgatgttc tcgccgttga tgcgtaccgc ataaacgacg agacacacat
     2821 cggatgcttg atcgtggcgg ccggtcgcag tcgagaatcg ccagccgtga ttcgcgcaca
     2881 cgacatggcc gcgcgcgatg atgccatccg acaaatcacc accgcgatgc acgcactggc
     2941 gctgcatcgc gaagtaccgg tcgccgtcca ggcccaggat catcggcacg ccctcggcct
     3001 cgactgcgat cacatcgccc ggcttcagtg cgccgacttt ggcgaccgga tgccagctgc
     3061 tcatgctcag accttggtcc acacggcgag ctcgcagcca cccggatcga cgaagtgaaa
     3121 ccgcttgccg ccggggaact cgtggcgcgc gacaatcgtc gctcccgccg cactcaccgc
     3181 gagctcgctc tgcgcgagat cgtcggagta gagaatgatg agcggtcc
//
LOCUS       DASUFX010000313         6615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_603077,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000313 DASUFX010000000
VERSION     DASUFX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603077"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFV99_12305"
     CDS             complement(<1..554)
                     /locus_tag="VFV99_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060140.1"
                     /translation="MDRRLLVMVVAFAGCSRTFSGTAVQPNPLVNQHETLRDSEKITI
                     VRGDMDLESPEPASGAGNASVAHNQSYPLINQANFTLVSRDRIRFHVQIDHKWQEYAD
                     LHTWEVTLVDDRGRRWVPESVDHDSTRIITTMWDREQRTAVCGGGGRDANGNCYNTIG
                     YQDDGWRRRQTLGSLSVYRGRADFV"
     gene            complement(610..1290)
                     /gene="gmk"
                     /locus_tag="VFV99_12310"
     CDS             complement(610..1290)
                     /gene="gmk"
                     /locus_tag="VFV99_12310"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5060141.1"
                     /translation="MKPTDRGVLVIVSSPSGAGKTTLTRKLLDEFTGQLEFSVSYTTR
                     PQRPGEVNGRDYWFVSPDEFEKMVERGEFAEHAFVFNNRYGTAQAPIDSALAAGRDVI
                     FDVDWQGGAALSGRWPKDSLKIFILPPSIEALETRLRQRATDAPDVIQRRLRKAKEEL
                     AHFNEYQHLIVNDDRDRAYQVLRALYLTRRYGRENRSDVPYQLSDLAKVVDSNQGTEA
                     HARKLVEP"
     gene            complement(1302..2189)
                     /locus_tag="VFV99_12315"
     CDS             complement(1302..2189)
                     /locus_tag="VFV99_12315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5060142.1"
                     /translation="MTGFGRGVAEQGRSRATVDVRTVNHRFLDLKLRGTVAPAVEDAI
                     TTRVRAALERGAVTVSVHITRDGGAGAMRIDSQAARVAYQQLGELAHTLSLPGPDLAL
                     VLAQPGVVVAADEDPRDDSDAPAVLAALDAALAQLAGMRDGEGQALARELLARIDELA
                     TLRIAIERLAADVPERVQKKLQQRLERLLQDETSDKTSDAAGWLDPARLAQEVALLAD
                     RADVTEELVRLASHLDQARALVNGSAAAGRRLDFLVQEIGRELNTIGSKSAATEISSA
                     IVEAKAVLEKVREQVQNVE"
     gene            2231..3367
                     /locus_tag="VFV99_12320"
     CDS             2231..3367
                     /locus_tag="VFV99_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060143.1"
                     /translation="MRSALLLIALAACGSEHAGPQPIRFLHTFGAEETEMFNALMAER
                     GIAVDASLVPFARGQQVIGEILRAGTACPDLIRIDATWLASLGPFAAVPDNLIGLDWL
                     PESKEMIATSAGNIGNAGAIPETVDGLVVIRDDATPAPASPAIADLIGAARAARHPGR
                     PHPLGLRVDGYWLLPWLRADNIDLGFDSDGQPMIGHDGAARAMQAFASLFGDLAPSPP
                     PAGSEAPDEVRRWLGHELAYWVTGPWQVGALAERDHLAVSALAHAPRGGQLLMVPQCA
                     KRPTEGWKLAAELTSVEVQRAFAERFASVPSRKAALDASPPLVKQLYKALQTAEPLPR
                     SPLTPMLFDDLNPALAAVVAGDATPDEAVAGAARGWSRIAGQRK"
     gene            3364..5121
                     /locus_tag="VFV99_12325"
     CDS             3364..5121
                     /locus_tag="VFV99_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5060144.1"
                     /translation="MKRSLRWRVLLILSLAAVVPTVVVGVLAIMRARHDVENEVKRGS
                     LAHIRALGAALDGTLQGARRTIELAASQWADAPNDARETQLLIKRLRRGVPIVKSVSI
                     LDPDGKLLYGDPVPPNVDIGSHSFGGYIGDAIFTNAGTPTVHAVVQARGRTGELMGVV
                     VAALDLGFVHDVIAAARLGPGARVLVVDGTGVPVATSDTTTVSAHSLAGTDPAVDRAL
                     ASSIEGTLSANGMVSAYRNLSSYQSLRGVRWAIIHQQPEAQAYALARSTTRDTAIIGI
                     AALAIALALGAFFATKLTRPLADLAQRADAIAAGHTTDGAPVKGPGEIGVLAQSVDEM
                     AKRIAERAELAAALARGDRLASVGVMSAQVAHEINNPLTTVLGYAKLLLEDKPEGHPD
                     RTALELIADEAERMKKIIGGLLEYARAPRPSEPATVTATGAAAPGHSDPATVVRHVAA
                     LVAPQLKKAHAQLVTDVAEGKSIAIDAHALQQVLVNLLQNAAQALTGGGTITVSAKPA
                     AGGIATQLTIADDGPGIPAADQARVFDPFFTTKAPGTGTGLGLAVVKHLVATAGGSIE
                     VGDGPNGKGAEFRVVIPNA"
     gene            5219..6562
                     /locus_tag="VFV99_12330"
     CDS             5219..6562
                     /locus_tag="VFV99_12330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5060145.1"
                     /translation="MSSILVVDDERGIRALCSDVLGRAGHEVEAVDSAAGALAAITRK
                     KFALVLCDINLPDQDGVSILPKLLAGENPPAVLLITAYPSIDTAVRGMKLGARDYIGK
                     PFSPDELRLVVQRALAEDELQRKHGELTKRLAYGSMIGESPAMQQMRATIDKVAQSDA
                     TVLITGESGTGKELVARALHFAGRRAGGPFVPVNCGALVGTLLDSELFGHVRGAFTGA
                     DTSKRGLFVAADGGTLFLDEIGELPIELQPKLLRALQDGEVKPVGGTSAIRVDARVIA
                     ATNRTLEDQVKAGAFREDLFYRLNVITIEVPALRNRPGDIPLLAKHFAEQAALRAERG
                     RVSFTDAALAHLASQPWPGNVRELENTIERGVILAAGDVLDASDVAGPRPPTTGAAFA
                     TFTGDQVPTLDELERAHILKVLELCENQKTKAAQMLGINRTTLWKKLRQYGIEGD"
BASE COUNT         1069 a         2244 c         2230 g         1072 t
ORIGIN      
        1 acgaagtcgg cgcgtccgcg atacaccgag agcgagccga gcgtctgacg acggcgccag
       61 ccatcgtctt ggtagccgat cgtgttgtag cagttgccgt tcgcgtcgcg gccaccgccg
      121 ccgcagaccg cagtgcgctg ctcgcggtcc cacatcgtcg tgatgatgcg cgtgctgtcg
      181 tggtcgaccg attcaggaac ccagcgacgg ccgcggtcgt cgaccagcgt gacctcccac
      241 gtgtgaaggt cggcgtactc ctgccatttg tgatcgatct ggacgtggaa ccgaatgcgg
      301 tcacgcgaga ccaacgtgaa gttcgcctgg ttgatcagcg ggtaggactg gttgtgcgcg
      361 accgatgcgt tgccggcgcc gctcgccggt tcgggcgatt cgagatccat gtcgccgcga
      421 acgatcgtga tcttctcgga gtcgcgcagc gtctcgtgct ggttgacgag cgggttcggc
      481 tgcaccgccg ttcccgaaaa ggtgcgactg caacccgcga acgcaacgac catgacgagc
      541 agtcgacggt ccatattctc agggtggcgc gacggcgcct cgccgtcaac aagagaggaa
      601 cttgtagcgt tagggctcta ccaacttgcg agcgtgcgcc tctgtccctt ggttactgtc
      661 cacgaccttg gcgagatcgc taagttgata cgggacatct gaccggtttt cgcggccgta
      721 ccgacgggtc agatagagcg cgcgcagaac ctggtacgcc cggtcgcggt cgtcattgac
      781 gatcaagtgc tggtactcgt tgaagtgcgc gagctcttcc ttcgctttcc ggagcctgcg
      841 ctggatcaca tccggggcat cggtcgcacg ctgtcgcagc cgtgtctcga gtgcctcgat
      901 gctgggcggc aggataaaaa tctttagcga atccttgggc cagcggccgg aaagcgcggc
      961 gccaccctgc cagtcgacgt caaagatcac atcgcgtccc gcggcgaggg ccgaatcgat
     1021 cggggcttgc gcggtcccgt agcggttgtt gaacacgaac gcgtgctcgg cgaactcgcc
     1081 tcgctcgacc atcttctcga actcgtccgg gcttacgaac cagtaatcgc ggccgttcac
     1141 ttcgccaggc cgctgaggcc gcgtcgtgta cgacacggag aactcgagct ggcccgtgaa
     1201 ctcgtccagg agcttccgcg tcagcgtggt cttgccggcg ccggagggcg acgacacgat
     1261 gaccagcacg ccgcggtccg ttggcttcac ggtgctcggc atcattccac gttctggact
     1321 tgttcgcgca ccttctcgag caccgctttc gcctcgacga ttgcggagct gatctcggtg
     1381 gcggcggact tgctgccgat cgtgttgagc tcgcggccga tttcctggac gaggaaatcg
     1441 aggcgccggc ccgcggctgc gctgccgttg acgagcgcgc gcgcttgatc gaggtgactc
     1501 gcgagccgca ctagctcctc ggtgacgtcg gcacggtcgg cgagcagcgc gacttcctgg
     1561 gcaagccgcg ccggatcgag ccagccggca gcgtcgctcg tcttgtcgct cgtctcgtct
     1621 tgcagcaagc gttcgagccg ctgctgcagc ttcttctgca cgcgctcggg tacatccgcg
     1681 gcgagccgtt cgatcgcgat gcgcagcgtg gcgagctcgt cgatgcgcgc cagcagctcg
     1741 cgcgcaagcg cctggccttc gccgtcgcgc atccctgcga gctgagcgag tgcggcgtcg
     1801 agcgcagcga gcacggcagg tgcatcgctg tcatcgcgcg ggtcttcgtc agccgcgacc
     1861 acgacgcccg gttgagcgag cacgagtgcg agatcggggc ccggcaggct gagcgtatgc
     1921 gcgagctcgc cgagctgttg atacgccacg cgtgcagcct gcgaatcgat gcgcatcgcg
     1981 ccggcgccgc catcgcgcgt gatgtgcacc gagacggtga ctgcaccgcg ttcgagtgcc
     2041 gcgcgtacgc gcgtagtgat cgcatcttcg accgcgggcg cgaccgtgcc gcgcagcttg
     2101 agatccaaga agcggtggtt gacggtgcgg acgtcgacgg tcgcgcgcga gcggccctgt
     2161 tcggccacgc cgcgtccaaa gccggtcatg ctgcgcaccg agcagagata tcacgatgta
     2221 gagtctccac gtgcgctcgg cgctgctctt gatcgccctt gctgcctgcg gcagcgagca
     2281 tgcaggcccg cagccgatcc gctttctgca cacgttcggc gccgaagaga ccgaaatgtt
     2341 caacgcactg atggcggagc gcgggatcgc cgtcgacgcg tcgctcgtcc cgttcgcgcg
     2401 cggccagcag gtgatcggtg aaattctgcg cgctgggacc gcatgtccag atctgatccg
     2461 catcgacgcg acctggctcg ccagcctcgg accgttcgcg gcggttcccg acaacctcat
     2521 cggcctcgat tggttgcccg agagcaaaga gatgatcgcg acgtcggcgg gcaacatcgg
     2581 caacgccggc gcgattcccg agaccgtgga cgggctcgtc gtgatccgcg acgacgcgac
     2641 accagcaccg gcgagtccgg cgatcgccga tctgatcggc gccgcgcgcg cggcacgtca
     2701 tcccggacgg ccgcatccac ttggcctccg cgtcgacggc tactggctgt taccgtggtt
     2761 gcgcgccgac aacatcgatc tcggcttcga ctctgacggc caaccgatga tcggccacga
     2821 cggtgccgcc cgcgcgatgc aggcgttcgc gtcgctgttc ggcgatctcg caccatcgcc
     2881 accgcccgcg ggcagcgagg cacccgacga agtgcggcgc tggctcggcc acgagctcgc
     2941 gtactgggtc accggaccgt ggcaggtcgg cgcgctcgcc gagcgtgatc acctcgcggt
     3001 cagcgcgctc gcgcatgcgc cgcgcggtgg tcagctgctg atggttccgc aatgcgcgaa
     3061 gcgccccacc gagggctgga agcttgcggc cgagctgacg agcgtcgagg tccagcgcgc
     3121 gtttgccgag cgcttcgcgt cggtgccgag ccgcaaagcc gccctcgatg cgtcgccgcc
     3181 gctcgtcaag cagctgtaca aggcgttgca aaccgccgaa ccgttaccgc gttcgccgct
     3241 gacgccgatg ttgttcgacg atctgaatcc cgcactcgcg gcggtcgtcg ccggtgatgc
     3301 gacaccggac gaagccgtgg ccggcgcggc gcgcggctgg agccggatcg cagggcagcg
     3361 caaatgaaga ggtcgctgcg ctggcgggtg ttgctgatcc tgtcgctcgc ggcggtggtg
     3421 ccgacagtcg ttgtcggtgt gctcgcgatc atgcgcgcgc gccacgacgt cgagaacgaa
     3481 gtgaagcgcg gctcgctcgc gcacattcgc gcgctcggcg cggcgctcga tggcacgctg
     3541 cagggcgcgc gtcgcacgat cgagctcgcg gcatcacaat gggccgatgc accgaacgac
     3601 gcgcgagaga cgcagctgct catcaagcgg ctcagacgcg gcgtgccgat cgtcaagagc
     3661 gtctcgatcc tcgatcccga cggcaagttg ctctatggcg acccggtgcc accgaacgtc
     3721 gacatcggct cgcacagctt cggcggctac atcggcgacg ccatattcac gaacgcgggt
     3781 acgccgaccg tgcacgcggt cgtccaggcg cgcgggcgca cgggcgagct gatgggcgtc
     3841 gttgtcgcgg cgctcgacct cggcttcgtc catgacgtga tcgccgcggc gcgactcggg
     3901 cccggtgcgc gcgtgctcgt cgtcgatggc actggagtgc ccgtcgcgac cagcgacacg
     3961 acgactgtat ctgcgcactc gctcgcgggt accgatccgg cggtcgaccg cgcactcgcg
     4021 tcgagcatcg agggcacgct gtccgcgaac ggcatggtga gcgcgtatcg caacctgtcg
     4081 agctatcaat cgctgcgtgg cgtgcgctgg gcgatcatcc atcagcaacc ggaggcgcaa
     4141 gcctacgcgc tcgcacgcag cacgacgcgc gacaccgcga tcatcggcat cgcagcgctc
     4201 gcgatcgcac tcgcgctcgg tgcgttcttc gcgacgaagc tgacgcgccc actcgcagac
     4261 ctcgcgcagc gcgccgatgc gatcgcggcc ggtcacacga ccgacggtgc gccggtgaag
     4321 ggcccgggtg agatcggcgt gctcgcgcag tcggtcgacg agatggcgaa acgaattgcc
     4381 gagcgtgccg agcttgctgc ggcgctcgca cgcggcgatc ggctcgcgtc ggtcggcgtg
     4441 atgtcggcgc aggtcgcgca cgagatcaac aacccgctca cgaccgtgct cggctacgcc
     4501 aagctgctgc tcgaggacaa accggaaggt catcccgatc gcaccgcact cgagctcatc
     4561 gccgacgaag ccgaacgcat gaagaagatc atcggcggcc tgctcgagta cgcgcgcgcg
     4621 ccgcggccca gcgagccggc gacggtcacc gcgacgggtg ctgccgcgcc gggtcactcc
     4681 gatccagcga cggtcgttcg ccacgtcgcc gcgctggtcg cgccgcagct caagaaggcg
     4741 catgcacagc tcgtcactga cgtggcggag ggtaagtcga tcgcgatcga cgctcacgcg
     4801 ctccagcagg tgctcgtcaa tctgctgcag aacgcggcgc aggcgctgac cggtggcggc
     4861 acgatcacgg tgtcggcgaa gccagccgcg ggcggtatcg cgacgcagct gacgatcgcc
     4921 gacgacgggc cgggcattcc cgccgccgat caggcgcgcg tgttcgatcc attcttcacg
     4981 acgaaggcgc caggtaccgg caccgggctc gggctcgcgg tcgtgaaaca tctcgtcgca
     5041 acggccggtg gatcgatcga agtcggtgac ggcccgaacg gcaaaggcgc cgagttccgc
     5101 gtcgtgatcc cgaacgcgtg acgttgccat ttacaacgcc gtccccggta cagcatttcg
     5161 tccacgctac cccaagtcga ccgtagcgcg cgctcggcgg tcgtgatacc cacgcgacgt
     5221 gagctcgatc ctcgttgtcg acgacgaacg cgggatccgc gcgctgtgct ccgacgtgct
     5281 cggccgcgcg ggtcacgagg tggaggcagt ggattccgca gcaggcgcgc tcgccgcgat
     5341 cacgcgcaaa aagttcgcgc tcgtgttgtg cgacatcaac cttcctgatc aggacggcgt
     5401 ttcgatcctg ccgaagctgc tcgcgggtga gaacccgcct gcggtcctgc tgatcaccgc
     5461 gtacccgtcg atcgacaccg cggtgcgcgg catgaagctc ggcgcgcgcg attacatcgg
     5521 caaaccattc tcgccggacg agctccgcct cgtcgtgcaa cgcgcactcg ccgaggacga
     5581 gctccagcgc aagcacggcg agctcacgaa acgcctcgcg tacggctcga tgatcggcga
     5641 gtcaccggcg atgcagcaga tgcgcgccac gatcgacaag gtcgctcaat ccgacgcgac
     5701 cgtgctgatc actggcgaga gcggcaccgg caaggagctg gtcgcgcgtg cgcttcactt
     5761 cgcgggtcgc cgcgcgggcg gtccgttcgt gccggtcaac tgcggagcgc tcgtcggtac
     5821 gctgctggac agcgagctgt tcggtcacgt gcgcggtgcg ttcaccggtg ccgacacatc
     5881 gaagcgtggc ctgttcgtcg ccgccgacgg cggcacgctg ttcctcgacg agatcggaga
     5941 gttgccgatc gagctgcaac cgaaactgct ccgcgcgctg caagacggcg aggtcaagcc
     6001 cgtcggcggc accagcgcga tccgtgtcga tgcgcgcgtg atcgctgcga cgaatcgcac
     6061 gctggaagat caggtcaagg ccggcgcgtt ccgcgaagat ctgttctacc gcctcaacgt
     6121 catcacgatc gaagtgccgg cgctgcgtaa ccgtcccggc gacatcccgc tgctcgcgaa
     6181 gcactttgcc gagcaagccg cactgcgcgc cgaacgcggc cgcgtgtcgt tcaccgacgc
     6241 tgcactcgct cacctcgcgt cgcaaccgtg gcccggcaat gtccgcgagc tcgagaacac
     6301 gatcgagcgt ggagtgatcc tcgccgccgg agacgtgctc gacgccagcg acgtcgcggg
     6361 cccgcggcca ccgacgacgg gcgctgcatt cgcgacgttc accggcgatc aggtgccgac
     6421 gctcgacgag ctcgagcgcg cgcacatcct gaaggtgctc gagctgtgcg agaaccagaa
     6481 gacgaaggcc gcgcagatgc tcggcatcaa ccgcacgacg ctgtggaaga agctgcgcca
     6541 gtacggcatc gaaggcgact aaccacgccg ccggtagacc gccagccccg ccgcgatcga
     6601 gccaccgatc gcgag
//
LOCUS       DASUFX010000314        15191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_187816,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000314 DASUFX010000000
VERSION     DASUFX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187816"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..710
                     /locus_tag="VFV99_12335"
     CDS             162..710
                     /locus_tag="VFV99_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060146.1"
                     /translation="MTRALLLALAVIAIAAGCKGRKAEPPGLGHYKFGTTTRANVHDG
                     VCQPTELTDGRKAVWCFALPPIKVGKRIADVDAYFLGTDPPALPDNATKEQVDEFREA
                     QSKLPLIELQLKVRGCDEYETEQWISERFGKPDVDSKPTRKYWHNSFLWAAAFLPSEP
                     GRCVIHFLPVSENAEIDRLKQK"
     gene            complement(1002..1298)
                     /locus_tag="VFV99_12340"
     CDS             complement(1002..1298)
                     /locus_tag="VFV99_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060147.1"
                     /translation="MTVADDITNAILRQMSEKLSALEDIKGALGNVATELKQFKEETG
                     RNLSVTNQRLAIVEHKLIDLAAQLLLMGRYVKNSHSKAILELRKRVGRLEKKVG"
     gene            complement(1419..2444)
                     /locus_tag="VFV99_12345"
     CDS             complement(1419..2444)
                     /locus_tag="VFV99_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5060148.1"
                     /translation="MKSAVLFALALALVACRPADEAADDVDAAVVLPDAPDGMGCTAL
                     TPRTVPVESFVGPTGLETKMGQLIDGAQSTLDIQMYLFTVDSIAQKVIAAKQRGVVVR
                     MLLDKDEAGNMNVTPDFTAANVNWKWAPSLYTYSHAKYMIIDKSQAVIMSMNWNVDAM
                     RTERNYGIVDKDIEDIADVQSIFDADWALTNGTGNQAANLTCTRLVVSPTNSRQRLID
                     HIKTATTNLDVEVMYVSESSIRDEIIAAKQRGVTVRVIILDPAADEVVAFKAASIPVK
                     TPPSSFYLHAKLIIADTVAFVGSENMSFTSLTKNREVGALVFEPTAFMPIKTQFEADW
                     TASIAVP"
     gene            complement(2564..3064)
                     /locus_tag="VFV99_12350"
     CDS             complement(2564..3064)
                     /locus_tag="VFV99_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5060149.1"
                     /translation="MGEQRSDFRPNDTGELKERWRSSSPDRRLNVTAQREAIVEMFLR
                     TRDHVSIDELLTKVRKRQPRVGYATVYRTLKLLVDSGLAVERQFGDGQARYEVVGDHH
                     DHLICVKCGLILEFEDDEIERLQDRIASRLGGFTVLRHRHELYGLCPKAAGEATGMCP
                     NEQRKK"
     gene            3151..3390
                     /gene="xseB"
                     /locus_tag="VFV99_12355"
     CDS             3151..3390
                     /gene="xseB"
                     /locus_tag="VFV99_12355"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5060150.1"
                     /translation="MADAPDLGFDAILVRLREVVQKLESGELSLEQSLAVYEEGVQLA
                     RRGQQLLASAEKRVEILVSAAGGVQTVPFDEPNGT"
     gene            3391..4260
                     /locus_tag="VFV99_12360"
     CDS             3391..4260
                     /locus_tag="VFV99_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5060151.1"
                     /translation="MSATLAPSSAPTQLAAFLEEARVAVDAELDRRLRVVPAARSESE
                     PLHSWGDDPGRLAEAMHYAATGPGKRLRPALVIAACEACGGTRAQAMPAAAAIEMLHA
                     YTLVHDDLPAMDDDDERRGRPTVHIAFGEAIAILAGDGLLTAAFGALAELGPRAGDAV
                     AVLARRAGVHELLGGQAIDLTTARETLHDLGAIERMHAAKTGALFAAAAELGAISAGA
                     DNHTRNALARFGMAIGIAFQHADDRDDAELTEHAERAAERMRVLCGEARQAVQPLGST
                     LLDSLASWIAARA"
     gene            4281..5204
                     /locus_tag="VFV99_12365"
     CDS             4281..5204
                     /locus_tag="VFV99_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5060152.1"
                     /translation="MPVPPDEWEEETTAATDLEELREKLSTVGVARTKPVLTVLTGSA
                     SGKQYKVSKGTAVIGRAPNVELRVEDDGISRAHARIRAETVRAWVEDLKSRNGTFVNG
                     TRITATTELRDGDKIQVGRGTVIRFGFQDDLDESFHENLMSSALRDGLTKLFNKRYFL
                     ERLDSELKFAARHGTALSLLMLDLDHFKKINDSRGHVAGDTVLATAAAVLQRSARNED
                     VVARFGGEEFGIILRAIELESALVMAERVRKLVEATPIAIDGGKILHATISIGVACYP
                     ATQANTPEELVDAADKALYRAKNAGRNRVER"
     gene            5255..7609
                     /locus_tag="VFV99_12370"
     CDS             5255..7609
                     /locus_tag="VFV99_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5060153.1"
                     /translation="MGRIAWLVCAVLCACSGDDSHRHTKTPKNPGPPTTPAAAVLPGG
                     LSESMVASYWTTPDELAGAAKFALEDYKAAADAFGRALAATKDPDRAARLDLILGLCY
                     ERIDDAANAAPHFTAAYAKLPELADYIGYHAARALSLTQKYDEANALAQKVAKDSTVG
                     ADTEILIGDLIRRKSDWAAAVTHFRGYLTAHPNGPFRSEARFDLAEALENSKGDQQEA
                     IKLYRQITVDDPLSSWTTKARARLTALKVAETFTAAEQIARGMELFDAMRNPESEAAF
                     TAALADPKVTAAEKCVASYHKAQSRFKARDRAGAAPMFDAASVACKAAANTDLWIKSE
                     YQAGRSYAFNGQHETAITHYQAAQAVDSKHSYADDALLREAEEWASLNNGAKVEEVLS
                     SLPTKFPTGDNVAEAMWRLGWRAWLDKKYDDAIKWWKEQIRLVPRDDNYYGEGQAQYW
                     LGRAYMAKNQKADAIAQWQTTVRSYPAAYYALLALNRLQENAPEDYKKLVAEISTDPA
                     GYDPKAPAFVFKPRPEWSAPGFQRAMELLRLGIGSAAEPELKKLGLAAPPGKSRVNDA
                     DQIEKLWAMAFLFDRAGRYATSIWPTRWHILDYRTQWPIGANRARWLIAYPRGYYELL
                     SRHAQLNQVPFAMQIGIVREESGFNPLTESYANAIGLTQMIPPTAKDFSKGTGIDPTR
                     ENLRDPEKNVTIGSRFLGHLYKTFKGFTSLIPTGYNAGPTAVKRMLKLRGTYAPDEFV
                     ESIVDDQARNYTKRVTGSFFTYSWLYEKTIPTMPNQFPADVLPK"
     gene            complement(7707..8246)
                     /locus_tag="VFV99_12375"
     CDS             complement(7707..8246)
                     /locus_tag="VFV99_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX5060154.1"
                     /translation="MGNAMKLIGCVVVLLSGCIVGSDKKPVPADKTANAVIQPTTGNT
                     VTGNVDFKSHDGTVTMTVTIADAPEGDHGFHIHQNPACGNDGMDAGAHWDGETQGDSA
                     THGLPDSPMHHTGDTGNITIAADGSGTLTITSTTWTVGDGNLMTDVADHAVIFHMSAD
                     DGTMTSAGARLGCGIIVAN"
     gene            complement(8262..9023)
                     /locus_tag="VFV99_12380"
     CDS             complement(8262..9023)
                     /locus_tag="VFV99_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5060155.1"
                     /translation="MKLSGSVALVTGGASGLGAATVRRLVAGGARAVIVDRDEARGRE
                     LATELGQQFAKADVTDAAQVEAAIQQAGTLGPLRVVVSCAGVGWASRTLDKTGKPHDL
                     ELFKTVVGVNLIGTFNVLRLGAAAIAKTEALEHGERGVIINTASVAAFDGQIGQIAYS
                     ASKAGVAGMTLPAARDLAPVGIRVLTIAPGIFDTPMLGALPEDKRAALAADVVFPKRL
                     GSPDEYGALVVAIVENGYLNGETIRLDGSLRMPPK"
     gene            complement(9040..9822)
                     /locus_tag="VFV99_12385"
     CDS             complement(9040..9822)
                     /locus_tag="VFV99_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060156.1"
                     /translation="MRARGWWLVLVALSTSAAADPKPKTVDIKPFRDNMIVLTDADGA
                     LYLVVPGSDGHVFYGVGGKNKNLYDQIIIGRYSDGSTGAWDVSVWAPRVPHVQPGGIQ
                     KKADGTYQKTCGNDSKTPLTELTGDKAKAVIDKGAFFSSAIIRRPYMLARDDSGVYYY
                     VDVIRKEYGGKGYRVFVGKKGAMKQKPLTDVASDTAGDIFATKTGDLRIVRDTSEGKD
                     TVQWVKGEKRSALVSLDVDANSALIYSDLGVYSFIGSICESI"
     gene            9892..11661
                     /locus_tag="VFV99_12390"
     CDS             9892..11661
                     /locus_tag="VFV99_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060157.1"
                     /translation="MIADTYTVEALIGRGGMGAVFLASHKRLPGKQVAIKVLHAELAG
                     EEVLARFKREAEIASRLGHPNIVQVHDFNVMPDGTPYLVLEYLRGESLAQRLKGGPMP
                     LEATLSTVRQVGSALAVAHREGIVHRDLKPQNIFLVPTEVDGRIVEIAKVLDFGISKI
                     RGSTTVKTQDSALLGTPQYMAPEQATGQHSSVDDRTDVFAFGAIVYEMLSGQPAFSGE
                     TIPEVVFKVVYEQPMPLAQRVSGLPERITAAVHRAMAKNADERFPNVGAFVEALTGQP
                     LASTRPGASIPPPDLGFAAGSRAPTTGQDAFAQTMGSGDHGPAAQPSADGSPPTAHVP
                     PPAHRLGSAPTIDSQSQQLGHAGTVSAHPPPVKRKTGLIVALVLGGAVIAAVAMYVAM
                     KKPDDSKQVAQVTEPSDKMVAADAATRAEVPVVVADAAAGVEKKAEIDIGSGSSRSGT
                     TSPQEEAKPAKQDDRSAKQDKQPKHEDKPPKQDGKPPAVDEETDDGDEAVKKKLSDAE
                     AALAANNWNRAEQLANAVTRSQDANARQKLRATMIFGTVQCLGRNDEGRARAELRKLT
                     VAPKMRRKLIDACHSAGYLQNVR"
     gene            complement(12308..12688)
                     /locus_tag="VFV99_12395"
     CDS             complement(12308..12688)
                     /locus_tag="VFV99_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060158.1"
                     /translation="MFDAPMVVFRSFAIGLLAACFALLVMRPEVHVTHVVPAQVATLA
                     LSQRQEPAPTIVDVAPGISAAQLALAIRLAPGEQITSIDDVAVSGNVGAGLVLASRSL
                     RSGEYIDFSVAGPRGERRVLALLH"
     gene            complement(12865..13341)
                     /gene="bfr"
                     /locus_tag="VFV99_12400"
     CDS             complement(12865..13341)
                     /gene="bfr"
                     /locus_tag="VFV99_12400"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5060159.1"
                     /translation="MKGNEEILELLNQLLTNELTAINQYFIHAKMCENWGYERLAHKI
                     REESIDEMRHADDIISRILFLEGVPNLQRYHKLRVGETVKEQLESDLQLEYAAIAFLN
                     QGIVAARNAKDNATEDMFTKILVSEEEHTDWIETQLELIRQVGEQNYLTQQIKKTS"
     gene            complement(13447..13686)
                     /locus_tag="VFV99_12405"
     CDS             complement(13447..13686)
                     /locus_tag="VFV99_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5060160.1"
                     /translation="MIVCMCHPISDRDVDAIIDDGARTVEEISRRCGAGTGCGACVDE
                     LRERLVAKGAAGCSRDCAQEHRDTSSELVSVRLAR"
     gene            13773..14642
                     /locus_tag="VFV99_12410"
     CDS             13773..14642
                     /locus_tag="VFV99_12410"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HEX5060161.1"
                     /translation="MYRCLLLIALAVFGCNKGDEGETQVKPPDLHVVSLGNEPRRLLR
                     YQAPKGTTTTLEMAVDVEVTAGDMGGPMPTIVMTLAMQVEDVLPTGMKLRTTVVDAMA
                     RDKDETRVAPNALTGPLEQIKGIVLTTTMTPTGRLIGTKVDPGSKQLPPTAQTQLAAL
                     TASLDQLMMPLPEEPVGVGAVWRNSKPLEQNGMKMIAVNTVSITAITGDKVSYSIETQ
                     VHGDDQTIQDSGLSVTVKDITGTGTGIGVIDLRTLALTSELSTEFRSAMQAAGEGSAT
                     PMRMAIATHVTPK"
     gene            complement(14643..15113)
                     /locus_tag="VFV99_12415"
     CDS             complement(14643..15113)
                     /locus_tag="VFV99_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060162.1"
                     /translation="MLRLLPLLIALVACGPKTTTDVKTGGGTGGSKVVGAGSGSAKPA
                     AGPVGTVPDVGCLQPSCAYHAGANGYFTCLSGGAGACFHFGGPCTPTDNCMYDAVDRT
                     YKQCARSVEGSCQQWGAACAPASACMFSATDGLHHKCDEVAGGSCKKYGALCAP"
BASE COUNT         2644 a         4997 c         5003 g         2547 t
ORIGIN      
        1 accctgtcgg gccagggtac gctcgacgag aagcacgtcg aggccaaagg cacgttcgag
       61 ctcgatatcc gcaagctggg actctcggcg ccacgcttct tcatgttcaa gatggaggat
      121 gaagtgaccg tcgaagtgac cgtgcgcgga gccgtcaaag cgtgacccgc gcgctcttgc
      181 tcgcgctggc ggtcatcgcg atcgccgccg gctgtaaagg tcgcaaggcc gagccgcccg
      241 ggctcggtca ctacaagttc ggcacgacga cacgcgcgaa cgtccacgac ggcgtgtgcc
      301 agccgaccga gctgaccgac ggccgcaagg cggtctggtg cttcgcgctg ccgccgatca
      361 aggtcggcaa gcgcatcgcc gacgtcgacg cgtacttcct cggcaccgat ccgcccgcgc
      421 tacccgacaa cgcgacgaag gagcaagtcg acgagtttcg cgaggcgcag tcgaagctcc
      481 cgctcatcga gctgcagctc aaggtgcgcg gctgcgacga gtacgagacc gagcaatgga
      541 tcagcgagcg ctttggcaag cccgacgtcg acagcaagcc gacccgcaag tactggcaca
      601 actcgttctt gtgggccgcg gcgtttctcc cgagcgagcc cggccgctgc gtgattcact
      661 tcctgccggt cagcgagaac gccgagatcg atcggctgaa gcagaagtag gccgacggcg
      721 cgtagtccgc gaccaccgcc ggctctttcg ctcgagctgg cggcagatcc gacgtgattc
      781 acgcagcatc gcacgcacgt cggtgatggg atcgcccgag ttgaactcgg caagtacgtc
      841 acgcaggtct ttgccgagga gcgtgcgtga ctcgcgcagc atggctttga cgtcctcgat
      901 gggatcgccg gagttgtact gcgcaagtac ctctcggtca tcgaacccga gagcgcggag
      961 ctttgctttc caacgccgat gggagcgctc caacgaagtc atcaacccac tttcttctcg
     1021 agtctgccga ctcgtttgcg cagctcgaga atcgccttgc tgtggctgtt cttcacgtag
     1081 cggcccatca gcagaagctg tgcagcgaga tcgatcaact tgtgttcgac gattgcgagc
     1141 cgttgattcg tgaccgagag gtttcgtcca gtctcttctt tgaactgctt gagctcggtc
     1201 gcgacgtttc cgagcgcgcc cttgatgtcc tctagcgcgc tcagtttctc gctcatctgg
     1261 cgcaggatcg cgttcgtgat gtcatcggcc acggtcacct cctgaagggt accgcgcgcg
     1321 tatcagcaag cccaaggcca ttcgtaagca cgcggatcga tacgcgcgtt gccgtgcgcg
     1381 tccgaaatgt ccggccgtga cgcggactgc gcgtccgctt acgggacggc gatcgacgcg
     1441 gtccagtcgg cttcgaactg cgtcttgatc ggcatgaagg cggtcggctc gaacacgagc
     1501 gcgccgacct cgcggttctt cgtcagcgac gtgaatgaca tgttctcgct gccgacgaac
     1561 gcgacggtgt ccgcaatgat cagcttcgcg tgcagataga acgagctcgg cggcgtcttg
     1621 accggaatgc tcgcggcctt gaacgcaacc acttcgtcgg ccgcgggatc gaggatgatc
     1681 acgcgcacgg tgacgccacg ctgcttcgcg gcgatgatct cgtcgcgaat cgagctctcg
     1741 ctgacgtaca tgacctcgac gtcgaggttc gtcgtcgcgg tcttgatgtg atcgatgagg
     1801 cgctggcgcg agttcgttgg cgacacgacg aggcgcgtgc acgtgaggtt cgcggcctga
     1861 ttgccggtgc cgttcgtgag cgcccagtcg gcgtcgaaga tcgactgcac gtccgcgatg
     1921 tcttcgatgt ccttgtcgac gatgccgtag ttgcgctcgg tgcgcatcgc atcgacgttc
     1981 cagttcatcg acatgatcac ggcctgcgat ttgtcgatga tcatgtattt cgcgtgcgag
     2041 tacgtgtaga gcgacggcgc ccacttccag ttgacgttcg ccgccgtgaa atccggcgtg
     2101 acgttcatgt tgccggcttc gtccttgtcg agcagcatgc gcacgacgac gccgcgctgc
     2161 ttcgccgcga tgaccttctg cgcgatcgaa tcgacggtga acagatacat ttggatgtcg
     2221 agcgtcgact gcgcgccgtc gatcagctgg cccatcttcg tctcgagccc ggtcggtccg
     2281 acgaacgatt cgaccggcac cgtgcgcggc gtcagtgcgg tgcagcccat gccgtcgggt
     2341 gcgtccggca acacgaccgc ggcgtcgacg tcatctgcag cttcgtccgc agggcggcag
     2401 gcgacgagcg cgagcgcgag cgcgaagagg acagccgact tcatcggccg ccgactatgc
     2461 aggcgcatgt agcgattccc aagcacaaac gtcacgtgtc cgctgtcacg tcgctgcaaa
     2521 ccgccggttg cagaggagcg acgggagatc gatggacccc caactacttt ttgcgttgct
     2581 cgttcgggca cataccggtg gcttcgccgg cggccttcgg acacaagccg tagagctcgt
     2641 ggcggtgacg cagcacggtg aaaccgccga ggcgcgacgc gatcctgtct tgtaggcgct
     2701 cgatctcgtc gtcctcgaac tcgaggatga ggccgcactt cacgcagatc aggtgatcgt
     2761 gatgatcgcc gaccacctcg tagcgcgcct ggccgtcacc gaactggcgc tcgaccgcga
     2821 gcccgctatc gaccaggagc ttgagcgtgc ggtagacggt cgcgtagcca acacgcggct
     2881 ggcgcttgcg gaccttggtc aagagctcgt cgatcgatac gtgatcgcgc gtacgcagga
     2941 acatctccac gatggcctcg cgctgcgccg tcacgttgag cctgcgatcc gggctggagc
     3001 tgcgccaccg ctccttgagc tcgccggtat cgttgggacg gaagtccgag cgctgctccc
     3061 ccataccgtt tcagcttccc cgcaactgct cgacgcgtca agcgatctcg cgcgggtgac
     3121 ttttcagttc ctcccgggta aggtgccgcc gtggccgacg cacccgacct cggtttcgac
     3181 gcgatcctcg tgcgtttgcg cgaggtcgta cagaagctcg agtcgggtga gctgtcgctg
     3241 gagcagtcgc tcgcggtcta cgaagaaggc gtgcagctcg cgcgacgcgg ccagcaactt
     3301 ctcgcatccg cggagaagcg cgtcgagatc ctggtgagcg ctgccggtgg cgtgcagacg
     3361 gtcccgttcg acgagcccaa tggaacgtaa atgtcggcga cgctggctcc ctcgagtgca
     3421 cccacgcagc tagctgcgtt tctcgaggaa gcgcgtgtgg ctgtcgacgc cgagctcgat
     3481 cgccgactgc gcgtcgttcc cgcggctcgg agtgaatccg agccgctcca ttcctggggc
     3541 gacgatcccg gacgactcgc cgaagcgatg cattatgcag cgacggggcc aggcaagcgg
     3601 ctgcgccctg ccctcgtgat cgcagcgtgc gaggcgtgcg gcggcacgcg cgcgcaagcg
     3661 atgcccgcgg ccgctgcgat cgagatgctg cacgcgtaca cgctcgtgca cgacgaccta
     3721 ccggcgatgg acgacgacga cgagcgccgc ggccggccga ccgtgcacat cgcgttcggt
     3781 gaagccatcg cgatcctcgc cggtgatggt ttgctcacag ccgcgttcgg cgcgctcgcc
     3841 gagctcggtc cgcgcgcggg agatgcggtg gcggtgctcg cacgacgtgc tggcgtgcac
     3901 gagctactcg gaggacaagc gatcgatctg acgaccgcgc gcgagacgct gcacgatctc
     3961 ggcgcaatcg aacgcatgca cgcggccaag accggagcgc tgtttgccgc tgccgccgag
     4021 ctcggcgcga tttcagccgg agcggacaat cacactcgta acgcgctcgc ccgctttggc
     4081 atggcgatcg gcatcgcgtt tcagcacgcc gacgatcgag atgatgccga gcttacagaa
     4141 catgcagagc gagccgccga acgcatgcgc gtgttgtgtg gtgaggcgcg tcaggcagtc
     4201 cagccacttg gctcgacact gctcgactcg ctcgcgagtt ggattgcagc gcgagcgtga
     4261 ctcacggtac gaattagttc gtgcccgtgc ctcccgacga gtgggaagaa gagaccacgg
     4321 cggcgactga tctcgaggag ctccgcgaga agctgtcgac cgtcggtgtc gctcggacca
     4381 agccggtgct caccgtattg accggcagcg cgtcgggcaa gcagtacaag gtctcgaagg
     4441 gcactgccgt gatcggccgc gcgccgaacg tcgagctgcg cgtcgaagac gatggcatct
     4501 ctcgcgcgca tgcgcgcatt cgcgccgaga ccgttcgcgc gtgggtcgag gacctcaaga
     4561 gtcgcaacgg cacgttcgtg aacggcacgc ggatcaccgc gacgaccgag ttgcgcgacg
     4621 gcgacaagat ccaggtcggc cgcggcaccg tgatccggtt cggcttccag gacgatctcg
     4681 acgagtcgtt ccacgagaac ttgatgtcgt cggcgctgcg cgacggcctg acgaagttgt
     4741 tcaacaagcg ctacttcctc gagcgcctcg acagcgagct gaagtttgct gcgcggcatg
     4801 gcaccgcgtt gtcgctgctg atgctcgatc tcgatcactt caagaagatc aacgacagcc
     4861 gcggccacgt cgccggcgac accgtgctcg cgacggccgc cgccgtgttg cagcgctcag
     4921 cgcgcaacga agatgtcgta gctcgctttg gcggtgagga gttcgggatc atcctgcgcg
     4981 cgatcgagct cgagtccgca ctggtgatgg ccgagcgcgt ccgcaagctg gtcgaggcaa
     5041 cgccgatcgc tatcgacggc ggcaagatct tgcacgcgac gatctcgatt ggcgtcgcct
     5101 gctaccccgc gacgcaggcc aacacaccgg aagagctcgt cgacgccgcc gacaaggcgc
     5161 tctatcgggc gaagaacgca gggcgtaacc gcgtcgaacg ctaactctgg ccagggaatt
     5221 tgtcctggcg gatccacgtt gctagcttca ggcaatggga cgcattgcgt ggctcgtgtg
     5281 cgcggttctc tgcgcttgtt ccggtgacga ttcccatcgc cacacgaaga ccccgaaaaa
     5341 tcctggcccg ccaacgacgc cagccgctgc ggtgctaccc ggcgggctgt cggagagcat
     5401 ggtcgcgtcg tactggacga ctcccgacga gctcgccggc gccgcgaagt ttgcactcga
     5461 agactacaag gcagctgccg acgcgttcgg ccgtgcgctc gccgcgacga aggatccgga
     5521 tcgcgctgct cggctcgatc tgatcctcgg cctttgctac gagcgaatcg acgacgcggc
     5581 caacgccgca ccgcacttca cggccgcgta cgcgaagctg ccggagctcg ccgactacat
     5641 cggctatcac gccgcgcgcg ctctctcgct cacgcagaag tacgacgaag cgaacgcgct
     5701 cgcacagaag gtcgcgaagg actcgaccgt cggtgccgac accgagattc tgatcggcga
     5761 tctgatccgg cgtaagagcg actgggccgc agccgttacg cactttcgcg gctacctgac
     5821 ggcgcatccg aacggaccgt ttcgctcgga ggcgcgcttc gatctcgccg aggcgctgga
     5881 gaattcgaag ggcgatcagc aggaagcgat caagctgtat cgccagatca ccgtcgacga
     5941 tccgctgtcg tcgtggacga cgaaggcgcg ggcgcgcctc accgctctga aggtggccga
     6001 gacgttcacg gcggccgagc agatcgcgcg cggcatggag ctgttcgatg cgatgcgtaa
     6061 cccggagtcc gaggcggcat tcacggccgc gctcgccgat ccgaaggtca cagcggccga
     6121 gaagtgcgtc gcctcgtatc acaaggcgca gagccggttc aaagcgcgcg atcgcgccgg
     6181 cgcggcaccg atgttcgacg ccgcgtccgt tgcatgcaaa gccgcggcga acaccgatct
     6241 gtggatcaag tcggagtatc aggccggccg ctcgtacgcg ttcaacggcc agcacgagac
     6301 cgcgatcacg cactaccagg cggcgcaggc cgtcgattca aagcacagct acgccgacga
     6361 cgcgctcttg cgcgaggccg aggagtgggc gtcgctgaac aacggcgcga aggtcgaaga
     6421 ggtgctgtcg tcgttgccga ccaagttccc gaccggtgac aacgtcgccg aggcgatgtg
     6481 gcggctcggt tggcgagcgt ggctggacaa gaagtacgac gacgcgatca aatggtggaa
     6541 ggagcagatc cggctcgttc cgcgcgacga caactactac ggcgaaggcc aggcgcagta
     6601 ctggctcggc cgcgcgtaca tggcgaagaa ccagaaggcc gacgcgatcg cgcaatggca
     6661 gaccaccgtg cgcagctatc ccgccgccta ctacgcactg ctcgcgctca accggctgca
     6721 ggagaacgcg cccgaggact acaagaagct cgtcgccgag atctcgaccg acccggccgg
     6781 ctacgatccg aaagcaccgg cgttcgtgtt caagccgcgt cccgaatgga gcgcgcctgg
     6841 ttttcagcgc gcgatggagc tgctccggct cggcatcggc tcggctgcag agcccgagct
     6901 gaagaagctc ggcttggcgg cgccccccgg caagagccgc gtgaacgatg ccgatcagat
     6961 cgagaagctg tgggcgatgg cgtttctgtt cgaccgtgcc ggtcgctatg ccacgtcgat
     7021 ctggccgacg cgctggcaca tcctcgacta ccgcacgcag tggccgatcg gtgcgaatcg
     7081 cgcgcgctgg ctgatcgcgt atccgcgcgg ctactacgag ttgctctcgc gtcacgcgca
     7141 actcaaccag gtgccgttcg cgatgcagat cggcatcgtg cgcgaggaga gcggcttcaa
     7201 cccgctgacc gagagctacg cgaatgcgat cggcctcacg cagatgattc cgccgaccgc
     7261 gaaggatttc tcgaagggca ccggcatcga tccgacgcgc gagaacctgc gcgatccgga
     7321 gaagaacgtg acgatcggct cgcggttcct cggccacctc tacaagacgt tcaagggctt
     7381 cacgagcctg atcccgaccg gctacaacgc gggcccgacc gcggtgaagc gcatgctgaa
     7441 actgcgcggc acgtatgcgc ccgacgagtt cgtcgagagc atcgtcgacg accaggcgcg
     7501 caactacacg aagcgcgtca ctggctcgtt cttcacctac agctggctgt acgagaagac
     7561 gatcccgacg atgccgaacc agttcccggc cgacgtcctg ccgaagtgat cagttgcagc
     7621 gaacggttac cggcgatccg cggcaccggg cgctggtgcc ttcgcagtct tgtcgaggcc
     7681 gatctcgcga gcgtcgctgc ccaccgtcag ttcgcgacga tgatgccgca gccgaggcgc
     7741 gctccggccg acgtcatcgt gccgtcgtcg gcgctcatgt ggaagatcac cgcgtgatcg
     7801 gcgacgtcgg tcatgaggtt gccgtcgccg accgtccacg tcgtgctcgt gatcgtcagc
     7861 gtaccgctgc catcggcagc gatcgtgatg ttgccggtgt caccggtgtg gtgcatcggc
     7921 gagtccggca ggccgtgcgt tgccgaatcg ccctgggtct cgccatccca gtgtgcgccg
     7981 gcatccatgc cgtcgttgcc gcacgccggg ttctggtgga tgtgaaaccc gtggtcgccc
     8041 tcgggcgcgt ccgcgatggt cacggtcatc gtcacggtgc cgtcgtgcga cttgaagtcg
     8101 acgttaccgg tcacggtgtt ccccgtggtc ggctggatca ccgcgttggc cgtcttgtcg
     8161 gccggtaccg gcttcttgtc cgagccgacg atgcaaccgc tcaacaagac caccacacac
     8221 ccgatgagct tcatcgcgtt ccccatggtg gggccatcgt actacttcgg cggcatgcgg
     8281 agcgaaccat cgagccggat ggtctcgccg ttgagatagc cgttctcgac gatcgcgacg
     8341 accagcgcgc cgtactcgtc gggtgagccg aggcgcttgg ggaacacgac gtcggccgcg
     8401 agcgccgcgc gcttgtcctc gggcaacgcg ccgagcatcg gcgtatcgaa gatgccaggc
     8461 gcgatcgtga gcacgcgaat tccgacgggt gcgaggtcgc gggcggcggg cagcgtcatg
     8521 ccagcgacgc cggccttcga cgccgagtag gcaatttgcc cgatctggcc gtcgaacgcg
     8581 gcgacggacg ccgtgttgat gatcacgccg cgttcgccgt gctcgagcgc ttcggtcttc
     8641 gcgatcgcgg cggcaccgag ccgcagcacg ttgaacgtac cgatgaggtt cacgccgacc
     8701 acggtcttga acagctcgag gtcgtgcggc ttgccggtct tgtcgagcgt gcgcgatgcc
     8761 cagccgacgc cggcgcacga cacgaccacg cgcaatgggc cgagcgtgcc cgcctgctgg
     8821 atcgcggctt cgacctgcgc ggcgtcggtg acgtcggcct tcgcgaactg ctggccgagc
     8881 tcggtcgcga gctctcggcc gcgcgcttcg tcgcgatcga cgatcaccgc gcgcgcgcca
     8941 cccgcgacga gcctgcgaac cgtggcggcg ccgagacccg acgcgccacc cgtgacgaga
     9001 gcaaccgatc cggagagctt catgccccgg acagtacgtt cagatgctct cgcagatcga
     9061 gccgatgaaa ctgtaaacgc cgaggtccga gtaaatcagc gccgagttcg cgtcgacatc
     9121 gagcgaaacg agcgccgagc gcttctcacc cttcacccat tgcaccgtgt ccttgccctc
     9181 gctcgtgtcg cgcacgatgc gcagatcgcc ggtcttcgtc gcgaagatgt cgcccgcggt
     9241 atcgctcgcg acatcggtga gcggcttctg cttcatcgcg cccttcttgc cgacgaacac
     9301 gcgatagccc ttgccgccgt actccttgcg gatgacgtcg acgtagtaat aaacgcccga
     9361 gtcgtcgcgc gcgagcatgt acgggcggcg gatgatcgcg ctcgagaaga acgcgccctt
     9421 gtcgatgacc gctttcgcct tgtcgccagt gagctcggtg agcggcgtct tcgaatcgtt
     9481 gccgcacgtc ttctggtacg tgccgtcggc cttcttctgg atgccacccg gctggacgtg
     9541 cgggacgcgc ggcgcccaca cgctaacgtc ccacgcgccc gtgctgccgt cggagtagcg
     9601 gccgatgatg atctgatcgt agaggttctt gttcttgcca ccgacgccat agaacacgtg
     9661 gccatcggag cccggcacga cgaggtagag cgcaccgtcg gcgtcggtga ggacgatcat
     9721 gttgtcgcgg aacggcttga tatcgaccgt cttgggcttt gggtctgcgg cagcgctcgt
     9781 cgacagcgcc accagcacaa gccaccaacc tcgtgcacgc atcggtgtct cattatacgc
     9841 ttgccgccca tgcagaattc acctgcacga tcgagcctcg atgtggggtc ggtgatcgcc
     9901 gacacttaca cggtcgaagc gctgatcgga cgcggtggca tgggcgcggt gttcctcgcg
     9961 agccacaagc ggcttcccgg caagcaggtc gccatcaagg tgctccacgc cgagctcgcg
    10021 ggcgaggaag tgctcgcgcg gttcaagcgc gaggcggaga tcgcgtcccg gctcggccac
    10081 ccgaacatcg tgcaggtcca cgacttcaac gtgatgccag acggcacccc gtacctcgtg
    10141 ctcgagtacc tgcgtggcga gtcgctcgcc cagcggctga agggcggacc gatgccactc
    10201 gaggcgacgc tgtcgaccgt gcgccaggtt ggctcggcac tcgcggtcgc gcatcgcgag
    10261 ggcatcgtgc atcgcgatct caagccgcaa aacatcttcc tcgttccgac cgaagtcgac
    10321 ggccgcattg tcgagatcgc gaaggtcctc gactttggca tctcgaagat ccgtggctcg
    10381 acaacggtca agacgcagga cagcgcgctg ctcggcacgc cgcagtacat ggcgcccgag
    10441 caggcgacgg gccagcactc gagcgtcgac gaccgcaccg acgtattcgc gttcggcgcg
    10501 atcgtctacg agatgctgtc gggtcaaccc gcgttctcgg gcgagaccat tcccgaggtc
    10561 gtgttcaagg tcgtctacga gcagccgatg ccgctcgcgc aacgcgtgtc cggactgccg
    10621 gaacgcatca cggcggccgt gcaccgcgcg atggcgaaga atgccgacga gcgctttccg
    10681 aacgtcggtg cgttcgtcga ggcgctcacc ggtcagccgc tcgcgagcac gcgaccgggc
    10741 gcttcgatcc cgccgcctga tctcggcttc gccgccggct cgcgtgcgcc gaccacgggc
    10801 caggacgcat tcgcgcagac gatgggctcc ggcgaccacg gtcccgccgc gcaaccgagc
    10861 gccgatggtt cgccgccgac cgcgcatgtc ccgccgcccg cgcaccggct cggcagtgcg
    10921 ccgacgatcg attcgcagtc gcagcaactc ggacatgccg gcactgtctc cgcgcatccg
    10981 ccgccggtga agcgcaagac cggcctcatc gtcgcgctcg tgcttggcgg cgcggtgatc
    11041 gccgcggttg cgatgtacgt cgcgatgaag aagcccgacg actcgaagca ggtcgcgcag
    11101 gtcaccgaac cgagcgacaa gatggttgcc gccgatgctg cgacgcgcgc agaagtgccg
    11161 gtcgtagtcg ccgacgcagc cgccggtgtc gagaagaaag cggagatcga catcggctcg
    11221 ggctcgagcc gctccggcac gacgagccca caagaggaag cgaagccggc gaagcaggac
    11281 gacaggtcgg cgaagcagga caagcagccg aagcacgagg acaagccgcc gaagcaggac
    11341 ggcaagccgc cggctgtcga cgaggaaacc gacgatggtg acgaagcggt gaaaaagaag
    11401 ctgagcgacg cggaggccgc gctcgccgcg aacaactgga atcgcgccga gcagctcgcg
    11461 aacgccgtga caaggagcca ggacgcgaac gcgcgccaga agctgcgcgc gacgatgatc
    11521 ttcggaaccg tgcagtgcct cgggcgcaat gacgaaggcc gtgcgcgcgc cgagcttcgc
    11581 aagctgaccg tcgcgccgaa gatgcgccgc aagctgatcg acgcgtgcca cagcgctggg
    11641 tatctacaga acgttcgtta ggtcacgagt cgaagcgcgg ccgctcgccg cggaggctgg
    11701 acgggcaact gtctccgcag gacgtcggtc ttcgccccgt gatcggcgcg agcacgcccc
    11761 gctagccgcc ggtttcgcgg cgcaactgtc tctgcatgac gtcggccttc gccgtgtgat
    11821 cgggcgtcga cgcgagaacg cggcgagctg cacggagccg gcgagcgcgc gtccgacgtc
    11881 aacgcgagat gccgcgctcg ccgccggttt cgcggcgcaa ctgtctctgc aggacgtcgg
    11941 ccttcgccgt gtgatcgggc gtcgacgcga gaacgcggcg agtgcacgga gccggcgagc
    12001 gcgcgtccga cgtcaacgcg agatgccgcg ctggcggcgc agcggatggg gtcgatccgg
    12061 cgactgtaac taggtaggcc gcgccttgtt ccgcgcgtac cttgccctcg ctcgcgccgg
    12121 cgtcgaccgt tcttcggcag gcgccgggtc gacgcccatc cgcggagccg ccgagcgcgg
    12181 cgagcgtgcg ggcgctcttc gacggcgagc gtgcgtgcgg gcgctcttcg gcggcgagcg
    12241 tgcgggcgct cttcggcggc gagcgtgcgg acgctcttcg gcggcgctcg cgaggtgagc
    12301 cgcagcgcta gtgcagcagc gcgagcacgc gccgctcgcc gcgcggtcct gcgacggaaa
    12361 agtcgatgta ctcgcccgag cgcaggctgc gggatgccag cacgagcccg gcgccgacgt
    12421 tgccgctcac cgcgacgtcg tcgatcgatg tgatctgctc gccgggcgcg agccggatcg
    12481 cgagcgcgag ctgcgcggcg ctgatcccgg gcgcgacatc gacgatcgtt ggcgcgggct
    12541 cctggcgttg ggacaacgcg agcgtcgcca cctgcgcggg taccacatgc gtcacgtgaa
    12601 cctccgggcg catcacgagc agcgcgaagc acgcggccaa caggccgatc gcgaacgagc
    12661 ggaacacgac catgggcgca tcgaacatgg gcgcgtgatg ttgcaacgcg gctgccaggc
    12721 cgacagccgt gccttcgccg ggttagcggc attcgccgcg cgcacgcgtg ccgaattggc
    12781 agcgcgcgtc cgcgggcgag tgacgatttc tcgtcgcgca gcgcctgttg ggtcctgcct
    12841 gacgatttct cgtcgagaag cgtcttacga ggtcttcttg atctgctgtg tgaggtagtt
    12901 ctgctcgccg acctgacgga tgagctcgag ctgggtctcg atccagtcag tgtgctcttc
    12961 ttcggagacg aggattttcg tgaacatgtc ctcggtcgcg ttgtccttcg cgttccgcgc
    13021 ggcaacgatg ccctggttca agaacgcgat cgccgcgtac tccagctgca ggtcgctctc
    13081 gagctgctct ttcacggtct cgccaacgcg cagcttgtga tagcgctgca aattcggcac
    13141 accttcgagg aacaagatgc ggctgatgat gtcatcggcg tgtcgcatct cgtcgatcga
    13201 ctcctcgcgg atcttgtgcg cgagtcgctc gtaaccccag ttctcacaca tcttggcgtg
    13261 gatgaagtac tggttgatcg cggtcagctc gttcgtgagg agctgattca agagctccag
    13321 gatctcctcg ttgcccttca tgggcgcaag gtaacccagg tcttctgccg ggtggctagc
    13381 aggcgcccgc ggagatcgcg caaccgtgtc ggccgcgaag cgacgccgtt agcggaggcg
    13441 acacgcttag cgagcgaggc gaaccgaaac cagctcgctc gacgtgtcac gatgctcttg
    13501 cgcgcagtca cggctgcaac cggccgcgcc cttggcaacg agccgctcgc gcagctcatc
    13561 gacgcatgcg ccacagccgg tacccgcacc gcagcggcga ctgatctctt cgaccgtgcg
    13621 cgcgccgtcg tcgatgatcg catcgacgtc gcgatccgat atcgggtggc acatgcagac
    13681 gatcatctga aattgataat gcatttcaaa agcagaaacg tcaagccacc tcatttactg
    13741 gcgcaaccca ttggaagtcc tacactctag ccatgtaccg atgcctcctc ctcatcgccc
    13801 ttgccgtttt cggctgcaac aagggcgacg aaggcgaaac gcaggtgaag ccacccgacc
    13861 tccacgtcgt gagcttgggc aacgagccgc gccggctgct tcgctatcag gcacctaaag
    13921 gaaccaccac gacgctcgag atggcggtcg atgtcgaggt cacggcgggt gacatgggcg
    13981 gaccgatgcc aacgatcgtg atgacgctcg cgatgcaggt cgaagacgtg ctgcccaccg
    14041 gcatgaagct gcgcacgacc gttgtcgacg cgatggcacg cgacaaagac gagacgcgcg
    14101 tcgcgccgaa cgcgctcacg gggccgctcg aacagatcaa aggcatcgtg ctcactacca
    14161 ccatgacgcc gaccggtcgg ctgatcggca cgaaggtcga tcccgggagc aagcagctac
    14221 caccgaccgc gcagacgcag ctcgccgcgc tgactgccag cttggatcag ttgatgatgc
    14281 cgctccccga ggagcccgtc ggcgtcggcg cggtgtggcg caactcgaag ccgctcgagc
    14341 agaacggcat gaagatgatc gcggtcaaca ccgtgtcgat caccgcaatc accggcgaca
    14401 aggtgagcta ctcgatcgag acgcaggtgc acggcgacga tcaaacgatc caggacagcg
    14461 gcctgtccgt cacggtcaaa gacatcaccg gaaccggcac cggcatcggc gtgatcgacc
    14521 tgcgcacgct cgcactgacg agcgagctgt cgacggaatt ccgcagcgcg atgcaggcag
    14581 ccggcgaagg ctcggccacg ccgatgcgca tggcgatcgc gacgcacgtc acgccgaaat
    14641 agctacggtg cgcagagcgc gccgtacttc ttgcagctgc cgccggcgac ctcgtcgcac
    14701 ttgtggtgca ggccgtcggt cgcggagaac atgcacgcgc tggccggtgc gcacgccgcg
    14761 ccccactgct ggcaactgcc ctcgacggac cgcgcgcact gcttgtacgt gcgatcgact
    14821 gcgtcgtaca tgcaattgtc cgtcggcgtg cacgggccgc cgaagtggaa gcacgctccc
    14881 gccccgcccg acaggcacgt gaagtagccg ttcgcgccgg cgtgatacgc gcagctcggt
    14941 tgcaagcagc cgacgtcggg caccgtgccg acgggtcctg ctgcgggttt cgccgagccg
    15001 ctgcccgcgc cgacgacctt cgatccccct gtcccaccgc ccgtcttcac atcggtggtg
    15061 gtctttgggc cgcaggcgac gagcgcgatc aacagaggca ggagccgtag cactgccgga
    15121 atatagcgtg tcgttcgcca aagtcccggt gtagcgttgg cgagtgaggt ggatgctgat
    15181 cgcctgcttg t
//
LOCUS       DASUFX010000315        15113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_603392,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000315 DASUFX010000000
VERSION     DASUFX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603392"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFV99_12420"
     CDS             complement(<1..531)
                     /locus_tag="VFV99_12420"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5060163.1"
                     /translation="MVDEELEQFERDGFVILENVLSASELAELIAALAPYEENRPMGR
                     NAFEGQKSQRVYSLAAKGDVFLRLAEHPRVMALVERLLMPNFLLSTLQSIRLHPGEQS
                     QAWHTDDAFYFMPRPHRLPLAVSVIWAIEEFSEENGATEVIPGSHRWGQEHPDHREYD
                     SVAATMPAGSAIVFDGA"
     gene            complement(551..1249)
                     /locus_tag="VFV99_12425"
     CDS             complement(551..1249)
                     /locus_tag="VFV99_12425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX5060164.1"
                     /translation="MRIYVTSLAVGDLTVRGDEHHYLSHVRRARTGDAIELADGAGKR
                     ARGVIAKISDADTIVRVDAVEDIVDAPPRVRVLVPLIKGDRMDTCIEKLVEVGVDEIV
                     VWPAVRAVVKLDGARLVSRVEHYQALAQAAARQSGRASIPSVTSAASLAAALAALPAG
                     VRIVLDPAADRGAWPSSEDLTIISGPEGGFAPEEHDALATASFISLGLGPRVLRAETA
                     PVIAVALVRAATQS"
     gene            complement(1476..2351)
                     /locus_tag="VFV99_12430"
     CDS             complement(1476..2351)
                     /locus_tag="VFV99_12430"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5060165.1"
                     /translation="MGDWVEIIVPVGAADVDDVAALIADEIPAAAAGTELRGDEVVFW
                     VAASDVSAALIDTRNAVARWAAGGAAVDPVRVRSAAALPEAEWRDAWKKFFKVSRLTR
                     QFVVVPSWEKYEQQAEDVVIDLDPGMAFGTGTHASTRLVLEHMQVMADAGAAPARVLD
                     VGCGSGILAIAAVKRWPAATCVAIDNDPIAVQATAENALVNKVADRIDANATPVGDLI
                     ESFPLVLANIQAHVIRELKAALMKRCSGTLILSGLLTPQAQPLADEFVAAGMKLVKIR
                     ASAADPEWSSVVLEV"
     gene            complement(2420..2647)
                     /gene="rpsU"
                     /locus_tag="VFV99_12435"
     CDS             complement(2420..2647)
                     /gene="rpsU"
                     /locus_tag="VFV99_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5060166.1"
                     /translation="MENTFGKPVEVEVRDSLEKAMKILKQKMSKEGILQELKRRRFYE
                     KPSVKRKRKTREARKRLRREMKRRVTPAPTR"
     gene            complement(2710..2952)
                     /locus_tag="VFV99_12440"
     CDS             complement(2710..2952)
                     /locus_tag="VFV99_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060167.1"
                     /translation="MARVQQQTNQDEGRTMIDVTGVKVFSATKAKERELLGELITDWI
                     RSHPEHEIVDKIVTQSSDSEFHCLTITLFYKHNAKS"
     gene            3153..4496
                     /locus_tag="VFV99_12445"
     CDS             3153..4496
                     /locus_tag="VFV99_12445"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5060168.1"
                     /translation="MRVLLTVLAALWLTAPASAAPKPAIAQLPKIERFTLANGLEVAF
                     LAIDTAPVVAVQVWYHAGSKDEPRDRRGSAHMFEHMMFKGTKHVRAEAHAQFINGIGG
                     NVNATTDEDATHYINALPSDYLDFAVQLEAERMRNLLFRKEMIDTEREVVKEEVRQQE
                     NSPIAKGFLRFLAIAYTKHPYAWTAGGDIKDLDATTPADLKTFYDHYYQPNNAMLVIV
                     GKATLAQVKASVEKHFGPIAKAAEPPRPAKDAQEPAQTAKRREVVEPSGVGITFIGWH
                     IPAAKDKDVYALQVASIILGAGESSRLKVRLKTTDPKTKKPLALDGGAEALVREDPGM
                     VIALGAYLDKEQIDPVETAIFDEVKKLGTAGPTSQELRKAKNQVQSGFVFSLEQAQGL
                     ATAIGRSWILTGDPTAWLRDVDEIEKVSAADVKRVVQKYLAADKATIVVIPPKGR"
     gene            4498..6741
                     /locus_tag="VFV99_12450"
     CDS             4498..6741
                     /locus_tag="VFV99_12450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5060169.1"
                     /translation="MKKLVVLFSVAFSSLLACGPKPKPAEIPVLPGDGDKNVAKPTDT
                     GGGKTQANDPWVGRTDLIVPPAPKAPAKLELPGIDEFKLANGLQVFVVKNARLPVVSV
                     QLAIKAGRMDEPRARLGVAEATADMLVKGTTKRNAVALAKAIDFVGGTIAADSTFEAT
                     LVSCSVLARNLPTCLELMPEMITQSTFPDDELKKVKEGMVATVRNRLDDAGLLASVHV
                     QNLLWGNEHVRGWVNSEASINALRRDDLVAWQKTWFQPNNSLLVIAGDLDAKKVKSDL
                     ERAFGGWKKGTVPPTPSYKEPGLSGTRIRLVDKPGQTQTHIRIAQFGIKHDDPRFFDS
                     LVWNYALGGGAFSSRLMKVVRVEGGKTYGASSNFDRNLDKGSFVASTFTRNSEAVATT
                     KLMLGEIAKMAKDGPTQAEVDSAIANIAGGYGLRFQSAGDVGSALVGAELHGFGIEYL
                     SNFPLVVGKVDVESAKRAANEILDPKNYVVVMVGDAKDLEPQLEKAGWRYEKTTFAEP
                     ITKEPKQPEAPVDAKSLAAAKQILAEAVAAKGGKAKLAALKSLRMVATGTTKIQGQEL
                     PVEIERVFVVPDKMRIDATLAKQFKVIVGVDGKSGWQQAPTQSGGSQTVDFKGKDIEQ
                     AQFEAWREPELLLLKATDKDAKVAPAKDDTIDGKPHAAVRVGSPFGPDLVLYIDKKTK
                     LISRVTFSDGDAVQTDDFSDYKDVGGIKVAHKRASTGQGRVTNLEVTKVEWDPKIDPA
                     IFKKPAK"
     gene            6747..7916
                     /locus_tag="VFV99_12455"
     CDS             6747..7916
                     /locus_tag="VFV99_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX5060170.1"
                     /translation="MRFLAAFAILLGLSGVVRAQPRPLDRDELDAMVGEQPPQVILLT
                     FGVGARIFEKFGHAAVCLSYPETGREPVCFNYGVTNFNEGSAMIWNFLRTKQVFWVDP
                     ESWSDMISFYEEEDRDIYEQVLPLTPEQARTFEKGILTSLEEAHRYYHYDHFFDNCTT
                     RLRDMIDNVTSGKLRGGSDVAYPLTFRQLGYRGLSELPPLLALADFVIGRQTDDTPTL
                     WQAMFHPNVLRERVANTLGVQPQLIYKRHGRAFPTSGSLWRWQMLAIALVFTVPLVIA
                     TWRRRFQRVAVGWATFYLVFWGLLIWGLAIVSSIPGVRWNENILVLMPLDIVLPFLAI
                     ERRRRYARVRVAGLLLASALCAVGLLHQPLWIPILSALLPLSVIAFDQKIAPDPA"
     gene            7932..8189
                     /locus_tag="VFV99_12460"
     CDS             7932..8189
                     /locus_tag="VFV99_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060171.1"
                     /translation="MRRTRTITIAGREVSAEEALRFEVVRGVLMGTMTIVQGANKLHM
                     GIFELSRLVNGARTAVIKAFGEDALMRAREESQLSAAQAAS"
     gene            complement(8186..8662)
                     /locus_tag="VFV99_12465"
     CDS             complement(8186..8662)
                     /locus_tag="VFV99_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060172.1"
                     /translation="MIEHRLLDLAYTTDAVITVPLLAYYVPCRIEDAETVLDRLASEG
                     RLQLEVDDDGNVFYVFPNRQRIAAPLRPQHALMRREPSYDLETRQPPNPAAAAVLSMI
                     VPGAGQLYAGRPLSGIAWFALVTMGYLLLIVPGVLLHILCVASAAGAAYHWRPSMA"
     gene            complement(8698..10413)
                     /gene="ligD"
                     /locus_tag="VFV99_12470"
     CDS             complement(8698..10413)
                     /gene="ligD"
                     /locus_tag="VFV99_12470"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5060173.1"
                     /translation="MARRGAAEKLAKYRSMRDFTKTAEPSGTRAPSTRGSLYVIQQHA
                     ARRLHYDFRLELDGVLLSWSVPKGPSLSPSEKRLAVRTEDHPLDYGDFEGVIPKGEYG
                     GGTVSVWDHGRWQPEGDPREAMRKGRLTFTLDGKKLHGKWHLIRTKPQGKQEGWLLFK
                     SRDEAANENVDIVESRPESVISGRTLDEIAAESDRVWHSDRAHEPTVLEKRAARIAAV
                     AKHQPANEENPRKNLYRNVQLANEAAKRARKTRGAEPSIRAQTAGDVAALVKQLPLGF
                     ELTNLDKVLYPEQGITKAQLIAYLAVVAEWALPQLADRPLTLVRCPEGRHKQCFFQKK
                     IFPGSPKSIGTVKLREEDGTLVDYMKVTDMPGLVGLAQLGTLEIHTWGCHADKVERPD
                     FMVFDIDPDPDLEWERVALAAFDTRRRLGELGFTSFVKTTGGKGLHVCAPIKRTIDWD
                     SFKAFTKVFADKMAADEPKLYTSNMAKRARKGKVFVDYLRNGRNATFITPYSPRAREH
                     APVATPITWEELAHGVNPRSFTTKTVPARLAKLKQDPWAGIDDLDQAITASAWRALGA
                     KPPKR"
     gene            10478..11404
                     /locus_tag="VFV99_12475"
     CDS             10478..11404
                     /locus_tag="VFV99_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5060174.1"
                     /translation="MARAYIGTSGWVYAGWREHLYADTPTKKWLAVASRAFDALEING
                     SFYTQIKPETYQRWYEETPEAFRFALKGHRFVTHYKRLRDCRDSIIRLRDQVQPLREK
                     LAAVVWQLPSNFECNVERLADFMRALTAWRDVRHALELRHRSWFVPEVADVLRANHIA
                     VCMSDAPDFPIWREVTSDLVYVRLHGHTRKYASSYSEQSLRAWASATQRWLAQGRDVH
                     VYFDNDAEGHAVRNALRFQELVAGTPRRATPAVECVRPARRAAARSRPVASGSSRASP
                     WRRPTARPQSPASSGRRSIARTGSPGRRPSSR"
     gene            complement(11448..12893)
                     /locus_tag="VFV99_12480"
                     /pseudo
     CDS             complement(11448..12893)
                     /locus_tag="VFV99_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
     gene            complement(12992..13927)
                     /locus_tag="VFV99_12485"
     CDS             complement(12992..13927)
                     /locus_tag="VFV99_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060175.1"
                     /translation="MTKAHTLRVLLGALLTASAVAAACVHARDNGNYPVPELGRSTAQ
                     YPANPSVSPPDAAYTPPPPVEPVVTLTPVRPPEEPVEPTGEAHPGEPVTVPVGAGGYG
                     TTPAPYIPPNIPITPTTPPVENPPATPTPTPTPAPTNPQQPTNPQQPANPPQPTPTNP
                     TNPPPAPGTPPATPANPPATPTPTQPEPQPPPATPIPPGPAPGAPPATPAPPPATPAP
                     APGPMPAPTAPPSMMPMQAATAPSTIMPMQTPTAPGAPPPPPTGPRMDGGISIAPPRD
                     AGSIVPRADGGTPTPLPIRDGGIIGGPRGDAGIRR"
     gene            14064..14996
                     /locus_tag="VFV99_12490"
     CDS             14064..14996
                     /locus_tag="VFV99_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5060176.1"
                     /translation="MPARAIDTATIAFGLVSIPVKIYSTSEPTHEIHFHLIHEGCGER
                     LKQQYVCPRHGVVERDEMTKGFELTKGNFVELEKDELKALEAVASDEIAIREFVPASA
                     IDPIFVDRTYYLGPDKGGDRAYALLRDALEDAELVGVAAYSARGKQYITMVRPFEDGL
                     AMHQLRYADEVKPWSEVPLGKLPKAAASELKLASQIIDQLQHETFDASQYEDEVKDRV
                     RKLIAKKAKGGEIVAAPEAPKAVVTDLMEALKASLAGAGKAPAPEERVSVVRDGKRAG
                     ESHRGHARAKRSSRSRPHASRARSSRSHAKRAHR"
BASE COUNT         2578 a         4858 c         5042 g         2635 t
ORIGIN      
        1 cgcaccgtca aacacgatcg ccgagccggc aggcatcgtc gcggcgacgc tgtcgtactc
       61 gcggtgatca ggatgttctt ggccccagcg atgcgagccg ggaatcactt cggtcgcacc
      121 gttctcctcg gagaactcct cgatcgccca gatcacgctg actgcgagcg gcagccggtg
      181 cgggcgcggc atgaagtaga acgcgtcgtc ggtgtgccac gcctgcgatt gctcgccagg
      241 gtggaggcga atcgactgca gcgtcgacaa caggaagttc ggcatgagca ggcgctcgac
      301 gagcgccatc acgcgcggat gctctgcgag ccgcaggaac acgtcgccct tcgcagcgag
      361 gctgtacacg cgctggcttt tttggccctc gaacgcgttg cgtcccatcg gccggttctc
      421 ctcgtagggc gcgagcgctg cgatgagctc ggcgagctcg ctcgcagaga gcacgttctc
      481 gaggatcacg aacccatcac gttcgaactg ctcgagctcc tcatccacca tgagacgatg
      541 ctagcagcga tcagctttgc gttgcggcgc gcacgagcgc cactgcgatc accggagcgg
      601 tctctgctcg cagcacgcgt ggtcccagtc caagcgagat gaagcttgca gtcgcgagcg
      661 catcgtgctc ttcgggtgcg aagccgcctt cggggccgct gatgatcgtt agatcctcgc
      721 tgctcggcca cgcgccgcga tcggccgctg gatcgaggac gatccgaact ccggcgggca
      781 gcgctgcgag tgctgccgcg agcgacgccg cgctcgtcac cgacggaatc gacgcgcgtc
      841 cggactgtcg cgccgctgct tgcgcgagcg cctgatagtg ctcgacgcgc gaaacgagcc
      901 gcgcgccgtc gagcttgacg accgcacgca cggccggcca cacgacgatc tcgtcgacgc
      961 cgacctcgac gagcttctcg atgcaggtgt ccatgcggtc gcctttgatc agcggcacga
     1021 gcacgcgcac gcgcggcggc gcatcgacga tgtcctccac cgcatcgacg cgcacgatcg
     1081 tatcggcatc gctgatcttc gcgatcacgc cgcgggctcg tttaccggcg ccgtcggcga
     1141 gctcgatcgc atcgccggtc cgtgcgcgcc gcacatgcga gaggtagtga tgctcgtcgc
     1201 cgcgcacggt gaggtcaccg acggcgagcg aggtcacgta gatgcgcacg ggtgcagtct
     1261 agacccaaat ccgtcgtgct cgtagacgtg cacgatccgg cgacgctcat cggtgacgtc
     1321 gctcggacaa cccgcctagg ttgatcgata tcgctgccag actcggatgc tcgaaacccg
     1381 cttcgagccg cgatgctcgc cggggcgtcg atggctcgag gacgtcgcgc cctctatgac
     1441 gtacgccgct caaccgtcgt gtacgcaggt ggggtctaga cctcgagcac gaccgacgac
     1501 cactcggggt ccgccgcgct cgcgcgaatc ttcacgagct tcatgccggc cgcgacgaac
     1561 tcgtctgcaa gcggttgtgc ttgcggcgtg agcaggcccg acaagatcag cgtaccgctg
     1621 cagcgcttca tcagcgccgc tttgagctcg cggatcacgt gcgcctggat gttcgcgagc
     1681 acgagcggga acgattcgat gaggtcaccg accggcgtcg cgttcgcgtc gatgcgatcg
     1741 gcgaccttgt tgacgagcgc gttctcggcc gttgcctgga ccgcgatcgg atcgttgtcg
     1801 atcgcgacgc acgtcgcggc aggccagcgc tttaccgcgg cgatcgcgag aatgcccgag
     1861 ccgcaaccga cgtcgagcac gcgcgcgggt gctgcgcctg catcggccat cacctgcatg
     1921 tgctcgagca cgagacgcgt gcttgcgtgc gtgccggtgc cgaacgccat gccgggatcg
     1981 agatcgatca cgacgtcctc ggcctgctgt tcgtacttct cccagctcgg caccacgacg
     2041 aactggcgag tgagtcgact gaccttgaag aacttcttcc aggcgtcgcg ccactccgct
     2101 tcgggcagcg cagcagcgga acgcacgcgc accggatcga cggcagcacc accggccgcc
     2161 cagcgcgcga ctgcattccg cgtgtcgatg agcgcggcag agacgtcgct cgcggcgacc
     2221 cagaacacga cctcatcgcc gcgcagctcg gtaccggccg cggcggcggg gatttcatcc
     2281 gcgatcaacg cggcgacgtc gtcgacgtct gctgcgccca ccggaacgat gatctcgacc
     2341 caatcaccca tgaaacaaag aaaggccgct agtgcagcgg cctcgatacg ttaactcgct
     2401 cgatcgtcga gcgcgagaat cagcgcgtcg gagcgggcgt cacgcgacgc ttcatctcac
     2461 ggcgcagacg cttgcgggct tcgcgggtct tgcgcttgcg cttgaccgag ggcttctcgt
     2521 agaagcggcg gcgcttcagc tcctgcaaga tgccctcttt ggacatcttc tgcttgagga
     2581 tcttcatcgc cttctcgagg ctgtctcgaa cctcgacctc gacgggcttt ccgaacgtgt
     2641 tctccatggg gaggacagtg ggtacctgat caacccccag cggtcaagca ccgcttgggg
     2701 ggataggacc tacgacttgg cgttgtgctt gtagaacaac gtgatcgtta gacagtggaa
     2761 ctcagaatcc gacgactgcg tcacgatttt gtcgacgatt tcgtgctcgg gatggctgcg
     2821 aatccaatcg gtgatgagct cacccaagag ctcacgctct ttggccttcg tcgccgagaa
     2881 aaccttcaca ccggtcacgt cgatcatcgt ccgcccctca tcctggttag tctgctgctg
     2941 cactcgtgcc acggcacctc tcctcaacgt caaagcggct cttgggggcc gctgggtttc
     3001 aatggaagcc aactctgagg cacgatctcg tttcagtcaa tcgggataat tccaggctgg
     3061 gtgaactcag ggttaattac ctctttcggc cgtaagtact ggcatcccca ttacgttcgg
     3121 ctagtgtctc acccgctcga agggagaatc cgatgagagt tttgctgacc gtcctggcgg
     3181 cgctgtggct caccgccccc gcgtccgcgg cgccgaaacc ggcgatcgcc cagctcccca
     3241 aaatcgagcg tttcacgctc gcgaatggcc tcgaagtcgc gttcctcgcc atcgatacgg
     3301 cgcccgttgt cgcggtccag gtttggtatc acgccggcag caaggacgag cctcgcgacc
     3361 gtcgcggctc ggcccacatg ttcgagcaca tgatgttcaa ggggacgaag cacgtccgcg
     3421 ccgaggcgca cgcgcagttc atcaacggca tcggcggcaa cgtcaacgcg acgaccgacg
     3481 aagacgccac gcactacatc aacgcgctgc cgagcgacta cctcgacttc gcggtgcagc
     3541 tcgaagccga gcgcatgcgc aatctgcttt ttcgcaaaga gatgatcgac accgagcgcg
     3601 aggtcgtgaa ggaggaggtt cgccagcagg agaactcgcc gatcgcgaaa ggcttcctgc
     3661 gcttcctcgc gatcgcgtac acgaagcatc cgtacgcgtg gaccgccggc ggcgacatca
     3721 aggatctcga cgcgacgacg ccggccgact tgaagacgtt ctacgaccac tactaccagc
     3781 cgaacaacgc gatgctcgtc atcgtcggca aggcgacact cgcgcaggtg aaggcgagcg
     3841 tcgagaaaca tttcggcccg atcgccaaag cagcggagcc gccgcgtccc gcaaaggatg
     3901 cacaggagcc ggcacagacc gcgaagcggc gcgaagtcgt cgagccaagc ggcgttggca
     3961 tcacgttcat cggctggcac attccggccg cgaaggacaa ggacgtctac gcgctgcagg
     4021 tcgcgtcgat cattcttggt gccggcgagt cgtcgcgcct caaggtgcgg ctcaagacga
     4081 cggacccgaa gacgaagaag ccactcgcgc tcgatggcgg tgccgaagcg ctcgtccgcg
     4141 aagacccagg catggtgatc gcgctcggcg cgtacctcga caaggagcag atcgatccgg
     4201 tcgagaccgc aatcttcgac gaggtgaaga agctcggcac ggcggggccg acgagtcagg
     4261 agctccgcaa ggccaagaac caggtgcaat cggggttcgt gttctcgctc gaacaagcac
     4321 agggcctcgc aaccgcgatc ggtcggtcgt ggatcctgac cggtgatccg accgcatggc
     4381 tgcgcgatgt cgacgagatc gaaaaggtct ccgccgccga cgtgaagcgt gtcgttcaaa
     4441 agtacctcgc cgccgacaag gcgacgatcg tcgtcatccc gccgaagggg cgctgacatg
     4501 aagaagctcg tcgtactgtt ctcggtcgcg ttctcctccc tcctcgcgtg cggccccaag
     4561 ccgaagccgg cggagattcc cgtcctgccc ggcgatggcg acaagaacgt cgcgaagcca
     4621 accgacaccg gcggcggcaa gacgcaggca aatgatccgt gggtcggccg caccgacttg
     4681 atcgtgccgc ctgcgccgaa ggcacccgcg aagctcgagc tacctggcat cgatgaattc
     4741 aagctcgcga acggcctgca ggtgttcgtc gtcaagaacg cgcgcctgcc ggtcgtcagc
     4801 gtgcaactcg cgatcaaggc gggccgcatg gacgagccgc gcgcccgcct gggcgtcgcc
     4861 gaagcaaccg cggacatgct ggtcaagggc accacgaagc gcaacgcagt ggcgctcgcg
     4921 aaggcgatcg atttcgtcgg cggcacgatc gcggcggact cgacgttcga ggcgacgctc
     4981 gtgtcgtgca gtgtgctcgc gcgcaacctg ccgacgtgtc tcgagctgat gcccgagatg
     5041 atcacgcagt cgacgttccc cgacgacgag ctcaagaagg tcaaggaagg gatggttgcg
     5101 accgtgcgca accggctcga cgacgcgggc ctgctcgcgt ccgtgcacgt gcaaaacctg
     5161 ttgtggggca acgagcacgt gcgcggctgg gtgaacagcg aagcgtcgat caatgcgctg
     5221 cgccgcgacg acctggtcgc gtggcagaag acgtggtttc agccgaacaa ctcgctgctc
     5281 gtgatcgccg gcgatctcga tgcgaagaag gtgaaatcgg atctcgagcg cgcgtttggc
     5341 ggctggaaga aaggcaccgt gccgccgacg ccgtcgtaca aggagccggg cctgtcgggc
     5401 acgcgcattc gcctcgtcga caagccgggc cagacgcaga cgcacatccg catcgcgcag
     5461 ttcggcatca agcacgatga cccgcggttc ttcgactcgc tcgtgtggaa ctacgcgctc
     5521 ggtggcggtg cgttcagttc gcgactcatg aaggtcgtgc gcgtcgaggg cggcaagacc
     5581 tacggtgcga gcagcaactt cgatcgcaac ctcgacaagg gctcgttcgt cgcgtcgacg
     5641 ttcacacgta acagcgaggc cgtcgcgacg acgaagctga tgctcggcga gatcgcgaag
     5701 atggcgaagg acggcccgac gcaagccgag gtcgattccg cgatcgcgaa catcgcgggc
     5761 ggttacggct tgcggttcca atcggccggc gatgtcggct cggcgctcgt cggtgcagag
     5821 ctgcacggtt tcggcatcga gtacctgtcg aactttccgc tcgttgtcgg caaggtcgac
     5881 gtcgagtcgg cgaagcgcgc ggcgaacgag atcctcgatc cgaagaacta cgtcgtcgtg
     5941 atggtcggcg acgcgaagga cctcgagccg cagctcgaga aggcgggctg gcgctacgag
     6001 aagacgacgt tcgcagagcc gatcacgaaa gagccgaaac agcccgaggc gcccgttgat
     6061 gcgaaatcgc tcgccgccgc aaagcagatc cttgccgagg ccgtcgccgc aaaaggcggc
     6121 aaggcgaagc tcgccgcgct caagtcattg cgcatggtcg cgaccggcac gacgaagatc
     6181 caaggccagg aactgccggt cgagatcgag cgcgtgttcg tcgtacccga caagatgcgc
     6241 atcgacgcga cgctcgcgaa gcagttcaag gtgatcgtcg gcgtcgacgg caagagcggc
     6301 tggcagcaag cgccgacgca aagcggcggc tcgcagaccg tcgacttcaa gggcaaggat
     6361 atcgagcagg cgcagttcga ggcctggcgg gagccggagc tcctgctgct caaggcgacc
     6421 gacaaggacg cgaaggtcgc acccgcgaag gacgacacga tcgacggcaa gccgcatgcc
     6481 gcggttcgcg tcggatcacc gtttggccca gacctcgttc tctacatcga caagaagacg
     6541 aagctgatca gccgggtcac gttcagcgac ggcgacgcgg tgcagaccga tgatttctct
     6601 gactacaagg atgtcggtgg catcaaggtc gcgcacaagc gggcgagcac gggccaaggc
     6661 cgggtcacga atctcgaggt cacgaaggtc gaatgggatc cgaagatcga tccagccatt
     6721 ttcaaaaagc cggctaaata acaagagtgc ggtttctcgc ggcgttcgcg atccttctcg
     6781 ggctgagcgg cgtcgttcgc gcgcagcccc ggccactcga tcgtgacgag ctcgacgcga
     6841 tggtcggcga acagccgccg caggtcatct tgctgacgtt cggcgtcggc gcgcgcatct
     6901 tcgaaaagtt cggccacgcc gcggtgtgcc tctcgtaccc ggagaccggg cgcgagccgg
     6961 tctgcttcaa ctacggcgtc acgaacttca acgaaggctc agcgatgatc tggaacttcc
     7021 tgcgcacgaa gcaggtgttc tgggtcgatc cggagagctg gtcggacatg atcagcttct
     7081 acgaggagga ggatcgcgac atctacgagc aggtgctgcc gctgacgccc gagcaggcgc
     7141 gcacgttcga gaagggcatc ctcacgtcac tcgaagaagc gcaccgctac taccactacg
     7201 accacttctt cgataactgc acgacgcggc tgcgcgacat gatcgacaac gtcaccagcg
     7261 gcaagctgcg gggcggtagc gacgtcgcgt acccgttgac gttccgccag ctcggttatc
     7321 gcggcctctc cgagctgcca ccattgctcg cgctcgccga cttcgtgatc ggtcgtcaga
     7381 ccgacgacac cccgacgctg tggcaagcga tgtttcatcc gaacgtgctg cgcgagcgcg
     7441 tggcgaacac gctcggcgtc cagccgcagc tgatctacaa gcggcacggc cgggcgtttc
     7501 cgaccagcgg ctcgctgtgg aggtggcaga tgctcgcgat cgcgctcgtc ttcacggtgc
     7561 cgctcgtcat cgcaacgtgg cgtcggcggt tccagcgcgt tgctgtggga tgggcgacgt
     7621 tctacctggt gttctggggc ttattgatct gggggctcgc gatcgtgtcg tcgattcccg
     7681 gcgtgcgctg gaacgagaac atcctcgtgt tgatgccgct cgacatcgtg ctgccgttcc
     7741 tcgcgatcga gcggcgccgc cgctatgcgc gggttcgcgt cgccggcctg ctcctcgcgt
     7801 cggcattgtg cgcggtcggg ctcctgcacc aacccctctg gattccgatt ttgagcgccc
     7861 tgttgcccct gtcggtaatc gcattcgatc aaaaaattgc ccctgacccc gcctagcgtg
     7921 agggtggtgg catgaggcgg acccgcacca tcaccatcgc aggccgagaa gtcagcgccg
     7981 aggaggcgct tcggttcgag gtcgttcgcg gtgtgctgat gggaacgatg acgatcgtgc
     8041 aaggggcaaa caagctgcac atgggcatct tcgagctgtc gaggctcgtc aacggcgcgc
     8101 ggactgcggt gatcaaagca ttcggcgagg acgcgctgat gcgtgcacgc gaagagagtc
     8161 agctgtcggc agcgcaagcg gcgagctagg ccatcgatgg ccgccagtga taggcggcgc
     8221 ccgcagcgga tgcgacgcag agaatgtgca agaggacgcc gggcacgatc agtagcaagt
     8281 agcccatcgt gacgagcgcg aaccacgcga tgcccgagag tgggcggcct gcgtagagct
     8341 gaccagcgcc gggcacgatc atgctgagca cggcggcagc ggctggattc ggcggctgtc
     8401 gtgtctcgag gtcgtacgag ggctcgcgtc gcatcagcgc gtgttgtgga cgcaacggcg
     8461 cggcaatgcg ctggcggttg gggaacacgt agaacacgtt gccgtcgtca tcgacttcga
     8521 gttgaagccg gccttcactc gcgagtcgat cgagtacggt ctcggcatcc tcgatcctgc
     8581 acggcacgta gtacgcgagg agcggtaccg tgatcaccgc gtcggtcgtg tacgcgagat
     8641 cgagcaagcg gtgctcgatc acagcagggt cgtttgtacg cggcatcgtc ggcattgcta
     8701 cctcttcggc ggtttggctc cgagcgctcg ccatgcgctg gcagtgatcg cttgatcgag
     8761 atcgtcgatg ccggcccacg ggtcttgctt cagcttcgcg agccgtgccg gcaccgtttt
     8821 cgtcgtgaag cttcgcgggt tgactccgtg cgcgagctct tcccaggtga tcggcgtggc
     8881 gacgggcgca tgctcgcggg cgcgcggcga gtacggcgtg atgaacgtcg cgttgcggcc
     8941 gttgcgcagg taatcgacga acactttgcc cttgcgcgcg cgcttcgcca tgttcgacgt
     9001 gtagagcttc ggttcgtcgg cggccatttt gtcagcgaac accttcgtga acgccttgaa
     9061 gctgtcccag tcgatcgtgc gtttgatcgg agcgcacacg tggaggcctt tgccgccggt
     9121 cgtcttgacg aagctcgtga agccgagctc gccgagccgt cgccgggtat cgaacgccgc
     9181 gagcgcgacg cgctcccatt cgagatccgg gtcgggatcg atatcgaaca ccatgaagtc
     9241 gggacgctcg accttgtcgg cgtggcagcc ccaggtatgg atctcgagcg tgccgagctg
     9301 cgcgagcccg acgaggccgg gcatgtcggt cactttcatg tagtcgacga gcgttccgtc
     9361 ttcctcgcgc agcttcacgg tgccgatcga tttcggcgaa ccggggaaga tcttcttctg
     9421 gaagaagcac tgcttgtgcc gaccctcggg gcagcggacg agcgtcagcg gccgatccgc
     9481 gagctgcggc agcgcccact cggcgacgac cgcgaggtac gcgatcagct gcgccttcgt
     9541 gatgccctgt tcgggataga gcaccttgtc gagattcgtc agctcgaagc cgagtggtag
     9601 ctgcttgacg agcgcggcga cgtcaccggc agtctgcgcg cgaatgctcg gttcggcgcc
     9661 gcgggttttg cgcgcgcgct tggctgcttc gttcgcgagc tggacgttgc ggtagagatt
     9721 cttgcgcgga ttctcttcgt tcgcgggctg gtgcttggcg acggctgcaa tgcgcgcggc
     9781 tcgcttctcg agcacagtcg gttcgtgcgc gcgatcgctg tgccacacgc gatcgctctc
     9841 ggctgcgatc tcgtcgagcg ttcgcccgct gatcacgctc tcgggacgcg actcgacaat
     9901 gtcgacgttt tcgttcgcag cctcgtcgcg actcttgaac agcagccagc cctcttgctt
     9961 gccctgcggc ttggtgcgga tgagatgcca cttgccgtgc agcttcttgc cgtcgagcgt
    10021 gaacgtcagc cggcccttgc gcatcgcttc gcgcggatca ccctcgggct gccagcgtcc
    10081 gtgatcccag acgctcaccg tgccgccgcc gtactcgccc ttcggaatga cgccttcgaa
    10141 atcgccgtag tcgagcgggt gatcctcggt gcgaacggcg agtcgtttct cgctcggtga
    10201 caggctcggg ccttttggca ccgaccacga gagcaacacg ccgtcgagct cgagtcggaa
    10261 atcgtagtgc agtcggcgcg cagcgtgctg ctggatgacg tagaggcttc cgcgggtgct
    10321 cggtgcgcgc gtaccgctcg gctcagcggt cttcgtgaaa tcacgcatcg accggtactt
    10381 ggcgagcttc tcggcagctc cgcgacgagc catacccgca caccttgcag agtcagtgcc
    10441 acgcgcgcgc ggcctgcgag atgcacggaa gcactgcgtg gcgcgcgcgt atatcggcac
    10501 cagcggctgg gtctacgccg gttggcgcga gcatctctat gccgacacgc cgacgaagaa
    10561 gtggctcgcg gtcgcatcgc gcgcattcga tgcgctcgag atcaacggtt cgttctacac
    10621 gcagatcaag cccgagacgt atcagcgctg gtacgaggaa acgccggaag cgtttcgatt
    10681 cgcgctgaaa ggccatcgct tcgttaccca ctacaagcga ctgcgcgact gtcgcgactc
    10741 gatcattcgc ctccgcgacc aggtgcagcc gctgcgcgaa aagctcgccg ccgtggtctg
    10801 gcagctgccg agcaacttcg agtgcaacgt cgagcggctc gcggacttca tgcgcgcgct
    10861 caccgcatgg cgcgacgttc ggcatgcgct cgagctgcgc catcgctcgt ggttcgtgcc
    10921 cgaagtcgcc gacgtgctcc gcgcaaacca catcgcagtg tgcatgagcg acgcgcccga
    10981 ctttccgatc tggcgcgagg tgacgagcga tcttgtttac gtgcgattgc acggccacac
    11041 tcgcaagtac gcttcgagct acagcgagca gagcctgcgt gcatgggctt ccgctacgca
    11101 gcgctggctc gcgcagggtc gtgacgtcca cgtgtacttc gacaacgacg ccgaggggca
    11161 cgccgtgcgg aacgcgttgc ggtttcagga gctcgtcgcg gggacgccga ggcgagctac
    11221 gccagcggtc gaatgcgttc ggccagctcg gcgggcggca gcgcgttcaa gacctgtcgc
    11281 ttccggatcc tcgcgcgcct cgccatggcg acggcccacc gcacggccgc aatcccccgc
    11341 gtcgagtggg cgtcgctcga tagcacgaac cggatccccc gggcggcggc cttcctcgcg
    11401 ttgatcggat cgaggtcgag gcgatacgga tcgccgttga tctcgatggc cgcacgcgac
    11461 tcggcgatcg cgtcgaagac ttcgtcgatg cgcaccgcga tcggatcgcg ccgcagcacg
    11521 agccggccaa gcgcgtggcc ccagatcttg aaatacggct ggcgcatcac ggtgacgagc
    11581 cgtttcgtca tcgcatcctc gtccgccttg tagcgctggt gcacgctcgc gatcacgaga
    11641 tcgagctcgg gcagaatctc ggcaggcacg tcgacgctgc cgtcgggaag gatgtcccct
    11701 tcggtaccgc gcaggatctt gaccggcgga ttctcgagcg ccgcgatctc ggcgtgctgc
    11761 ttgcgcagcg cttccgcgtc gaggccgccg gcgtacgtcg ccgccgcgct gtgatcggtg
    11821 atcgtgatgc cctgcatgcc gagctcggcg gccgctgcgg ccatctccgc aatcgagtgc
    11881 tttccatcgc tgtaggtcgt gtgacagtgg aacgccgtcg tcacatcggc gagcgtgacc
    11941 agatccgtga agtcgtcgcc tgcgtccgcg gcggcaagct cgtcggtggc gtcgcgcact
    12001 tccggcggca agaacggcaa gccgagcgcg cggtagacgt cgacctcgtc gggcgcgtcg
    12061 agcgcagtga gcacgacgcc acgagcctgc gcacgcgcct ccagctgcgc aacgtgctcc
    12121 ggcgatccgg tcgcgcagat cagcgcccag ccgaagtgcg cctttgtcgc gaaatggatc
    12181 tcgcaccgca tgccgttcgc gagccgcgcg gcgaggacgt cgttggttcg atcgacggac
    12241 gtgacgagtc cgtagttcgc gatccgatcg atcaccgcgt cgcgatggtc ggtctcgatc
    12301 gcgaacgcca ggtgctcgac gatctcgcac cagcgccgga cctggcccgc gatctcgacg
    12361 cggatcgccg catcgtgcgc gcgcagatag ctcgcgagtg actccgcatg ctgttcggca
    12421 tcgatgtgga tctcgcggct gccgcgtgtg cgccgctcat cgatcgcctt caggatcttc
    12481 tgctcgctca gcttgccgaa gcccttgagc tcgcgcagcg cgccggcctt cgcggcggca
    12541 gcgaccgcat cgagatccac gggcgcgagc gcctggtgta tcgctcgtgc cttctggaca
    12601 ccgaccgacg gcagctgtgc aagctcgacg atcacggctg gccacttcgc gcgcaacgtc
    12661 tcgagcagcg tgctggagcc gcgccgcgcc agatcaccga cggtgcgcgc gatcgatggc
    12721 ccgatgccgg gcagctcctc gagccggccc tcgtcgagta gccgatgcag accctttgcc
    12781 gcctcgatcg agcgcgcggc tttgtcgtac gcgatcgcgc gatggcgatc gccgtcgaga
    12841 tcgaagtaga ccgcgatctc gcgaagctgc gctgcgatca cggtcggctc catacctgcg
    12901 gtgtagcagg ttacggtagc gacgtgcagc gtgcggggtc tgctcgctcg tgatcggatc
    12961 gaacaaaccg actcgtgcgc gtgcggatgc cttagcggcg aatgccagcg tcgccgcgcg
    13021 gcccgccgat gatgccgccg tcgcgaatcg gtagcggagt cggcgtacct ccgtcggcgc
    13081 gcggcacgat cgagccggca tcacgcggtg gcgcgatcga gatgccgcca tccatgcgcg
    13141 gtcccgtcgg cggcggcgga ggtgcgcccg gcgccgtcgg cgtctgcatc ggcatgattg
    13201 tcgatggtgc cgtcgccgcc tgcatcggca tcatgctcgg cggcgcggtc ggtgccggca
    13261 tcggtcccgg cgctggtgcc ggcgtcgcgg gtggcggcgc tggcgttgcg ggaggcgccc
    13321 ctggcgccgg ccccggcgga atcggcgttg cgggcggcgg ttgcggttcc ggttgcgtgg
    13381 gcgtcggcgt cgccggtgga ttggccggtg tggcgggcgg cgttcccggc gcgggtggtg
    13441 gattcgtcgg attcgtcggc gttggttgcg gcggattcgc aggttgctgc ggattcgtcg
    13501 gttgctgcgg attcgtcggc gctggtgtcg gcgtcggcgt cggcgttgcc ggcggatttt
    13561 ccacgggcgg cgtcgtcggc gtgatcggga tgttcggcgg gatgtacggt gcgggtgtcg
    13621 ttccgtatcc accggcacca accggcaccg tcaccggttc gcctggatgt gcttcgccgg
    13681 tcggctcgac gggttcttcc ggcggtcgca ccggcgtcaa cgtcaccacg ggctcgacgg
    13741 gcggtggtgg tgtgtacgcc gcgtcgggcg gcgacacgct cgggttcgcc gggtactgtg
    13801 ccgtcgagcg cccgagctcc ggtactggat agttgccgtt gtcgcgcgcg tggacgcacg
    13861 ctgctgccac cgcactcgcg gtgagcagcg cgccgagcag cacgcgaagg gtatgggcct
    13921 tcgtcatggc gagtgcagag gctgcgaagc ccgtgccgct ggcagatttg ctatgcgtcg
    13981 tcggattccg tgcgccgtgg tcacgacgcg acgtgatgcg acgcgcccgc tggtctggta
    14041 cgcgactcgc agtaccgcga tgcatgcctg ctcgcgctat cgacacagca acgatcgcgt
    14101 tcggtctcgt ctcgattccg gtgaagatct attcgacgag cgagcccacg cacgagattc
    14161 actttcatct tatccacgag ggctgcggcg aacgactcaa gcagcaatac gtgtgtccac
    14221 gtcacggtgt ggtcgagcgc gacgaaatga ctaaaggctt cgagctcacg aagggcaatt
    14281 tcgtcgagct cgagaaagac gagctcaagg cactcgaggc tgtcgcgagc gacgagatcg
    14341 cgattcgcga attcgtgccg gcgtccgcga tcgatccgat ctttgtcgat cgcacgtact
    14401 acctcggccc cgacaagggc ggcgatcgcg cgtatgcgct gctgcgcgat gcactcgaag
    14461 atgccgagct cgtcggcgtc gcggcgtact cggcgcgggg caagcaatac atcacgatgg
    14521 tgcggccgtt cgaagacggc ctcgcgatgc accaactacg ttacgccgat gaagtgaaac
    14581 cgtggagcga ggtgccgctc ggcaagctgc cgaaggccgc agcatccgag ctcaagctcg
    14641 catcgcagat catcgatcag ctccagcacg aaacgttcga cgcgagccag tacgaggacg
    14701 aagtcaaaga ccgcgtgcgc aagctgatcg cgaagaaggc gaagggtggc gagatcgtcg
    14761 cggcaccgga agcgccgaag gctgtggtca ccgatctgat ggaggccctc aaagcgtcgc
    14821 tcgcaggggc ggggaaagcg ccggcgcccg aagaacgcgt ttctgtcgtg cgtgacggca
    14881 aacgcgcggg cgagtcgcat cgcggccacg cacgagcgaa acgatcgtca cggtcgcggc
    14941 cgcatgcgag ccgggcgcga agctcgcggt cgcacgcaaa gcgtgcacac cgttaacgcg
    15001 gcgcgttctt gatcgtcggc gcgtcgaggc cacctgcatc cgggaccggc ggtaagtcga
    15061 ccacgtcaga cacgccggca tccgtcggca tgttcgtcgg tggcggtgcg ggc
//
LOCUS       DASUFX010000316         8912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_326914,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000316 DASUFX010000000
VERSION     DASUFX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326914"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFV99_12495"
     CDS             complement(<1..823)
                     /locus_tag="VFV99_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5060177.1"
                     /translation="MGDAAVPASGLTSGDRAPPVARIARLFASPAAVLVILPALVIAA
                     GVTVMLLGRRATQDTAENMAKNQCVATAQAVRGDVESAVDQADPVFKMMSGLANSAMP
                     TQDALSRLHDIVIGRPVTNASIVFPIGVMWQTFRDKEHPKETRVQETRVGDTQTTRTS
                     FEIIDGDTKQMGDVEISDYDPRTRPYYIEAVKAKKRVWLPPRVFSSSGKTGVTVAEPV
                     LTPTGEVAAVLVLDYDAAELSALLRQPPLAGARSVVFTRDGTILAYPSAQVPEAAI"
     gene            complement(841..1551)
                     /locus_tag="VFV99_12500"
     CDS             complement(841..1551)
                     /locus_tag="VFV99_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060178.1"
                     /translation="MKHSVWLLVALTVVVWGGCFDEIDARWQLDHDHVVVARATPPRV
                     RSGDATNLDALVAHAGRGVSIENPLTAEVGSARFAAYLSQHDGAWTLTAPDAQVLASA
                     RPALGLPEDAPVPVEVLLTFPRSTDRDAPDPVRVKKTVWLGEPSENPVVPPVTIDGAP
                     AGDEVVVPLDRDVYLTVLAQGMRVNWLTSVGTLFQDDEATAFVHVAPDDKPAGELVVV
                     VRDDEGGVAWRVWPMRAE"
     gene            complement(1548..3695)
                     /locus_tag="VFV99_12505"
     CDS             complement(1548..3695)
                     /locus_tag="VFV99_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5060179.1"
                     /translation="MARVAAADPASVHGRVVDGQGNPLRGATVSVEGATTSVTTDAQG
                     RYVLTGVAPGASLVVLEDGYGAGLGTASESAGDIVLLADAQTNETIEVKGERPPQAQG
                     ATTLDRETLQRMPGARNDVMQTLSAMPGVASYPLPLGESGVVIRGSSPQDSKILIDDF
                     EVPMLYHYVGFRSVVPSDAIDSLEYVPGGFDVAYGRATSGIVSLTTRAGDAKRGQQFE
                     TSAGELGILGQGSFHHGHYMIAMRRSAIDLLLPLLLPDDLDLSLTTVPRFYDAQLRVD
                     YTLTDKWTLRLSGLGSDDAMELYASRDENPDKRFLNRGRFARLSASAHYHDGPLTAKL
                     AVSGIATQETFERGLYQHLDVTSPALTTRAEVSRTATAWAGLREIEWRTGVEAVTTRH
                     RIDIAAPREPREGEPPTSDDPMDVSTSYVGHVDTGNVAGWTSMKAALDERVRFNVGLR
                     VDAYTRADDLAVQPRGELQIKLAPKLVARVSGGYYSRPAEHQTELLDPALKAERSHQL
                     MGGVLYEPLAGLRLQGSLYYTQRTNLITRDAMGELGNEGRGATYGGELLGTLTRGPWF
                     GWLTYAYSHSTRVDLPGGTVRLFDYDQPHSLNAAASYRYKRWQFGARFRLHSGLPTTP
                     VDDAIYDSDANLYIPVYGAVNSERAPMHHQLDIRIDRTTHWGPVKITKFLDVQNVYLN
                     DTVVGYYYGFDYTQRAAFRTLPILPTAGLRGEF"
     gene            3830..4687
                     /locus_tag="VFV99_12510"
     CDS             3830..4687
                     /locus_tag="VFV99_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060180.1"
                     /translation="MHARSLCFAATLLFSGLAHADDSDTHIRAAYAANGDVARLTVTN
                     GGKKPCVKIDLKGKGKEIYGGEAAATIEAGHGLVVIAVSIDSEKKPFQIVTLKDGKLS
                     TPVGLARPAKRENYPFAVAATPTPDGFTVFFQEVETANPNEAHTYLVELDKDGAVTKE
                     PTEVQIPWALAAAAWNGNGYHLGLFYTGGGGVTLSMVSTSADGSPQQHPDWATPAGMI
                     TDVHLVANGKSIRAYYRGMGDRLYETDVSKIGQWGQVSQKAKDLGALSASQAIAISAK
                     GAVTKVKAK"
     gene            4918..5775
                     /locus_tag="VFV99_12515"
     CDS             4918..5775
                     /locus_tag="VFV99_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060181.1"
                     /translation="MADDSHVASVAGNCQPDHNASDIGTYNVVNGVITVSSPSIFYCS
                     VPLAKIGTIYALRLTASGNAHEVSAKLIRWSKGDGSTWEMGTISSSGSGVQGILQGIS
                     PETYDPTTYNYYFQVTISDSAGVFYGLEMIGTATDTAPHAYQTVASACQPEPNTIDSG
                     RYLVSSGGTVTFKGSATGTIKMYCQIPTKAPMFPWSQFRVYYKNNSSTSYIKASLIKM
                     NRSGGTRTALATVNSQSGTNDNAFRSVSTPTPSEDMDMNVYYYYIVLEISRTSSTQTP
                     KAYALTIDN"
     gene            6471..6602
                     /locus_tag="VFV99_12520"
     CDS             6471..6602
                     /locus_tag="VFV99_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060182.1"
                     /translation="MATCGRVGASARRRVAASLTTGSETTLGVIFLPQVVAMIHLYN"
     gene            6621..7838
                     /gene="mutY"
                     /locus_tag="VFV99_12525"
     CDS             6621..7838
                     /gene="mutY"
                     /locus_tag="VFV99_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5060183.1"
                     /translation="MDHANVASAVVTHYRRDRRDLPWRRTRDPYAIWVSEIMLQQTRV
                     ATVIPYWERWMAKFPTVRALAAAPLDDVLAAWAGLGYYSRARNLHAGAQALAIAGELP
                     KSASELRAVRGIGPYTAGAIASIAYGERTPLVDGNVARVLARLFAITDDIKSTAGQKL
                     LWQRAGALMAALPSDHAPGDLNQGLMELGATLCSPTSPRCLLCPLAAQCEAARTGRQD
                     ELPVVAARKKESELPILARAALWMADGDRVVLARRAPDGLFGGLWELPQGASRIEITK
                     ALGVTADATAVAYHDQTLSHRRLRIAVFRASVPRRLVATAVPGYDAISLVERATAKAL
                     GIAAATAAILSKYEDSPWSSIPKRSLSSPRATKRSSKASTTSGSTRATTTSSTRRSAR
                     RKGSTSSSTTRSK"
     gene            7727..8275
                     /locus_tag="VFV99_12530"
     CDS             7727..8275
                     /locus_tag="VFV99_12530"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX5060184.1"
                     /translation="MEGLNHLGFDTSDDNFVDTAKRAAKGLHELVHDQKQVRPAVEAL
                     LAKTFPAKYTEMVISKHNTAFGVCPHHLLPVVYRISMAYIPTEKVLGLSKLTRLARLI
                     ARGPRLQEDLTHELADILHEDLASQGSAVYIEGLHMCMAARGVGAHEARLVTSGVRGV
                     FLELATREEFIKLVIAPQTTVL"
     gene            8298..>8912
                     /locus_tag="VFV99_12535"
     CDS             8298..>8912
                     /locus_tag="VFV99_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5060185.1"
                     /translation="MRCALLHGFAGDPAAWDEVIDAWSLREAPIAVALPGHGGGPVLD
                     TWEDNLNVVAGAVVGCDVVVGYSLGARVALGLVAGGRAAHGVFIGVNPGINDSERAQR
                     REFDAAWTRLLRSSGIEAFHEAWMQQPLFATQSRVQTDKLERRRQRRLKLDAEQLARS
                     LEVMGIANMPDYWGAIPAHRDRIALIAGADDPKYVAISSGLPCAS"
BASE COUNT         1673 a         2894 c         2814 g         1531 t
ORIGIN      
        1 tgatcgctgc ctcggggacc tgcgccgatg gatacgcgag gatcgtgccg tcacgcgtga
       61 acacgacact gcgtgcgccc gcgagcggcg gctgccggag cagcgcggag agctcggccg
      121 cgtcgtagtc gagcacgagg acagcagcga cctcgccggt cggcgtgagc acaggttctg
      181 caaccgtgac accggtcttg ccggacgacg agaacacgcg cggtggcagc cagacgcgct
      241 tcttcgcctt cacggcctcg atgtagtacg ggcgggtgcg cggatcgtaa tcgctgatct
      301 cgacgtcgcc catctgcttc gtgtcgccgt cgatgatctc gaagctcgtg cgcgtcgtct
      361 gggtgtcacc cacccgtgtc tcttgcacac gcgtttcctt ggggtgctcc ttgtcgcgga
      421 acgtctgcca catgacgccg atcggaaaca cgatgctcgc gttcgtcacg ggccggccga
      481 tgacgatgtc gtgcaagcgc gatagcgcat cctgcgtcgg catcgccgag ttcgcgaggc
      541 cactcatcat cttgaacacc ggatcggcct gatcgacggc ggactcgacg tcaccgcgca
      601 cggcctgtgc ggtcgcgacg cactgattct tcgccatgtt ctcggcggtg tcctgcgtcg
      661 cgcgccgacc gagcagcatc accgtgacgc cggccgcgat cacgagcgcg ggcagaatca
      721 cgagcacggc cgcgggcgac gcgaacaagc gtgcgatgcg cgccacgggt ggtgcacgat
      781 cgccactcgt cagcccggaa gccggcacgg ccgcgtcacc cacgccgcga tgttacctga
      841 ttactcggcg cgcattggcc acacgcgcca tgcgacacca ccctcatcgt cgcgcacgac
      901 aaccacaagc tcgcccgcgg gcttgtcgtc gggagcaacg tgcacgaatg cggtcgcctc
      961 gtcgtcctgg aacagcgtgc cgacggaggt gagccagttc acgcgcatgc cttgcgcaag
     1021 gacggtcagg tagacatcgc gatcgagcgg caccacgacc tcgtcaccgg cgggcgcgcc
     1081 gtcgatcgtc accggcggta ctaccggatt ctccgacggc tcgccgagcc acacggtctt
     1141 cttcacgcgc accggatccg gcgcgtcgcg atcggtcgag cgcggaaacg tgagcagcac
     1201 ctcgaccggc accggtgcat cctcgggcaa gccgagcgcg ggtcgggccg aggcgagcac
     1261 ctgcgcgtcg ggtgcggtca gcgtccacgc gccgtcgtgc tgcgacagat acgccgcgaa
     1321 gcgcgccgag ccgacctcgg cggtgagcgg attctcgatc gagacgccgc gtcccgcatg
     1381 cgctaccagc gcgtcgaggt tcgtcgcatc acccgagcgg acacgcggtg gggtcgcgcg
     1441 cgcgacgacg acgtgatcgt gatcgagctg ccagcgcgca tcgatctcgt cgaagcaacc
     1501 gccccaaacg acaaccgtga gagccacgag cagccacacc gagtgcttca aaactcacct
     1561 cgtagtcccg cggtaggcag gatcggcagc gtgcgaaatg ctgcgcgctg cgtgtagtcg
     1621 aagccgtagt agtagccgac gacggtgtcg ttcaagtaca cgttctgcac gtcgaggaac
     1681 ttcgtgatct tcaccggtcc ccagtgcgtc gtgcgatcga tgcggatatc gagctggtgg
     1741 tgcatcggcg cgcgctccga gttcaccgcg ccgtagaccg ggatgtagag gttcgcgtcg
     1801 ctgtcgtaga tcgcgtcgtc gaccggcgtc gtcggcaaac cggaatgcag ccgaaaccgc
     1861 gcgccgaact gccagcgctt gtagcgatag ctcgccgcag cgttgaggct gtgcggttgg
     1921 tcgtagtcga acagccgcac ggtgccaccg ggtagatcga cgcgcgtcga gtgcgagtac
     1981 gcgtaggtga gccagccgaa ccatgggccg cgcgtcagcg tgccgagcag ctcgcctcca
     2041 tacgtcgcac cgcgaccctc gttgccgagc tcgcccatcg cgtcgcgcgt gatgaggttc
     2101 gtccgttgtg tgtagtagag cgagccttgc aggcggagac cggcgagcgg ctcgtagagc
     2161 acgccgccca tcagctgatg cgagcgctcc gccttcagcg caggatcgag cagctcggtc
     2221 tggtgctcgg caggccggct gtaatagccg cccgacacgc gcgcgacgag cttcggcgcg
     2281 agcttgatct gcaactcgcc gcgcggctgc accgcgagat cgtcagcacg cgtgtacgcg
     2341 tcgacgcgca ggccgacgtt gaaccgcacg cgttcgtcga gcgccgcctt catcgatgtc
     2401 cagccggcga cgttgccggt gtcgacatgg ccgacgtagc tcgtcgagac gtccatcgga
     2461 tcatcgctcg tcggtggttc accttcgcgc ggctcgcggg gcgcggcgat gtcgatgcga
     2521 tggcgcgttg tcacggcctc gacgccggta cgccattcga tctcgcgcag gcccgcccat
     2581 gcggtcgcgg tgcggctgac ctcggcgcgc gtcgtcagtg ccggcgacgt gacgtcgagg
     2641 tgctggtaga gcccgcgctc gaacgtctcc tgggtcgcga tgcccgatac cgcgagcttc
     2701 gcggtcagtg gaccgtcgtg atagtgcgcg gacgcgctca gtcgcgcgaa ccggccgcgg
     2761 ttcaagaacc gcttgtcggg attctcgtcg cggctcgcgt atagctccat cgcgtcgtcg
     2821 gaaccgagcc ccgacaggcg cagcgtccac ttgtcggtga gcgtgtagtc gacgcgcagc
     2881 tgggcgtcgt agaagcgcgg caccgtcgtc agcgacagat cgagatcgtc ggggagcagc
     2941 agaggcagca gcagatcgat cgccgaccgc cgcatcgcga tcatgtagtg gccgtgatga
     3001 aacgagccct ggccgagaat cccgagctcg ccggcggacg tctcgaactg ctggccgcgt
     3061 ttcgcgtcgc cggcgcgcgt cgtcagcgac acgatgccgc tcgtcgcgcg gccgtacgcc
     3121 acgtcgaatc cacccggcac gtactcgagc gaatcgatcg cgtcgctcgg cacgaccgaa
     3181 cgaaagccga cgtagtgata gagcatcggc acttcaaaat cgtcgatcag gatcttcgag
     3241 tcctgcggcg acgaaccgcg gattacgacg cccgactcgc cgagcggcag cgggtagctc
     3301 gcgacaccgg gcatcgccga cagtgtttgc atcacgtcgt tgcgcgcgcc cggcatgcgc
     3361 tgcagcgttt cgcgatcgag cgtcgtcgcg ccttgcgcct gcggcggtcg ctcgcctttg
     3421 acttcgatcg tctcgttcgt ctgcgcgtca gcgagcagca cgatgtcacc ggcgctttcg
     3481 ctcgcggtgc cgagtcccgc gccgtagccg tcctcgagca cgacgagcga tgcaccgggc
     3541 gcgacgccgg tcagcacgta gcggccttgc gcgtcggtcg tcacgctcgt ggtcgcgcct
     3601 tcaacggaca cggtcgcgcc gcgcagcgga tttccttgcc cgtcgacgac gcggccatgt
     3661 accgacgcag gatctgcagc ggcaacgcga gccacgagca cgatggcagc cccccaacgc
     3721 aagcgatgca ctgtgactca cccgtgggcc acgacaaccg tgaaattgct acgaaaaatc
     3781 cctcgaagat tttcggtcac gaaacgcgcg caccgccgca ctagacggca tgcacgcacg
     3841 atcgctttgc ttcgccgcga cgctgttgtt cagcggactt gctcacgccg acgatagcga
     3901 cacccacatc cgcgccgcct acgcggcgaa cggcgatgtc gcgcggctga ccgtcacgaa
     3961 cggcggcaag aagccgtgtg tgaagatcga tctcaaaggg aaaggtaaag agatctacgg
     4021 cggcgaagca gccgcgacga tcgaagcagg tcacggcctc gtcgtgatcg cggtgtcgat
     4081 cgattccgag aagaagccgt tccagatcgt cacgctcaag gatggcaagc tgagcactcc
     4141 ggtcggcctc gcacggccgg cgaagcgcga gaactatccg ttcgcggtcg cggcaacacc
     4201 gacgcccgac ggcttcaccg tgttcttcca ggaagtcgag accgcgaacc cgaacgaagc
     4261 acacacgtac ctcgtcgagc tcgacaagga cggcgcggtg acgaaggaac cgaccgaagt
     4321 gcagataccg tgggcgctcg ccgcggccgc gtggaacggc aacggctatc acctcggtct
     4381 gttctacacg ggcggtggcg gcgtcacgct ctcgatggtg tcgacgagcg cagacggctc
     4441 gccgcagcag catcccgact gggcgacgcc cgccggcatg atcacggacg tgcacctcgt
     4501 cgcgaacggc aagtcgatcc gcgcgtacta ccgcggcatg ggcgatcggc tctacgagac
     4561 cgacgtcagc aagatcggcc agtggggaca ggtctcgcag aaggcgaagg acctcggcgc
     4621 gctgtcggcg agccaggcga tcgcgatcag cgcgaagggc gcagtgacga aggtcaaagc
     4681 gaagtaagtt gactcgccgt ctggagccct gatgcggaac gagctaagtc gcgacgctgc
     4741 atgccgtaat aaacggcacg acagcgtcac cggttccatc tagcgtcctt tacagagccc
     4801 gcggaagtgg ttcgcggagc gaagacgact ttaggaggac agctcatgaa tacaaggcga
     4861 ttcagcctca ccgtaatcct cacgatgatg ataatcacat tcagaattag gacatcggtg
     4921 gcagacgatt cacatgttgc cagtgtcgcc ggtaattgcc agcccgatca caatgcttca
     4981 gatatcggca cgtacaacgt agtaaatggg gttattacag tttcgagtcc cagcatcttc
     5041 tactgctcag ttccactcgc aaaaatcggg acaatttacg cacttcgact aaccgcctca
     5101 ggaaacgcgc acgaggtcag cgccaagcta attagatgga gcaagggcga tggatcgacc
     5161 tgggagatgg gaaccatttc ttcgtccggt tccggcgtac agggaattct tcaaggtatc
     5221 agcccagaga cgtatgaccc cacgacatac aattactact ttcaagtcac cattagcgac
     5281 agcgcaggag tcttctacgg gctagaaatg atcggaaccg caaccgacac tgccccgcat
     5341 gcttatcaaa ccgtggcctc ggcgtgccag ccagaaccaa atacgatcga cagcggtcga
     5401 tacttggtca gcagcggcgg aaccgttacg ttcaagggca gtgccacggg aacgatcaag
     5461 atgtattgcc agattcctac caaggcaccc atgtttcctt ggtcacagtt tcgcgtctat
     5521 tacaagaaca atagcagcac gtcctacatc aaggcatcgc ttataaagat gaaccgatca
     5581 ggtggaacaa gaaccgctct ggccactgtc aactctcaaa gcggcacaaa cgataatgca
     5641 ttccgatcgg tatcaactcc tactccatcg gaagatatgg acatgaatgt atactactac
     5701 tacatagttc tcgaaatcag cagaacttca tcgacacaaa ccccgaaggc atatgcgctt
     5761 acaatcgaca actagatgga gagaaccatg aaacgatttc tactggctat catcctactc
     5821 gtcgggaccg cgaaggtgtc gaatgctgag cgcctgctct tcacgggcaa ctcgtgcgcg
     5881 ccgaccgact ttgactatta cttcaataca ttcagggtgc taagcaccgc ttcggacatc
     5941 ttcaagtgga agcccgattt gactgggacc atgaatctga cttgtccgat tccgcacaca
     6001 ctctcgtcat cgccgactac gttaacaatc tatttcgtca acgatgatga ttgcccgttg
     6061 tgtggcactg aagcgattac tgtcaactac gtcaagatga gcagaactac tggagcggtt
     6121 actaccgttg cgtcagtttc tagtacgtcc agtcctgtcg tgggttcagc cttcacttcg
     6181 gtgtccacaa cgttctcaga cacgtacgac accggcagct atatctattt cctgtatgta
     6241 tatatggcta gaaatacaag tctcaacgat caaatgctat tcggtgggga gctattttag
     6301 cagtcgctta ggaagacgcg ctgcgtacga agagcttcta agcaacgctt cgcaccaatt
     6361 gttccttggt tgctgaccac gcgaaccgac cgtggtgtgt cacgcactat cgccgacctc
     6421 gtttggcgac ggatgcgctt ggccaggtcg acccgtactg tgagtgctga atggcgacgt
     6481 gcgggcgcgt cggcgcgtcg gcgcgtcgcc gcgtcgccgc gtcgctcacg acgggctcgg
     6541 agaccacgct cggcgtgatc ttcctgccgc aggtcgtcgc gatgatccac ctctacaact
     6601 aagcgtggta ccaccacgcg gtggaccacg cgaacgtcgc gtccgccgtc gtcacgcact
     6661 atcgccgtga ccgccgcgac ctcccgtggc ggcgcacgcg cgatccgtac gcgatctggg
     6721 tcagcgagat catgctgcaa cagacgcgcg tcgccaccgt gatcccgtac tgggagcgct
     6781 ggatggcgaa gtttccgacg gtgcgcgcgc tcgcggccgc gccgctcgac gacgtgctcg
     6841 ccgcgtgggc cggcctcggc tactactcgc gcgcgcgcaa cctgcacgcg ggcgcacagg
     6901 cactggcaat cgcgggcgag ctgccgaaga gcgcgagcga gctgcgagcc gtgcgcggca
     6961 tcgggccgta cactgcaggt gcaatcgcat cgatcgcgta cggtgaacgc acgccgcttg
     7021 tcgatggcaa cgtcgcgcgg gtgctcgcgc gcttgttcgc gatcaccgac gacatcaagt
     7081 cgaccgccgg tcagaagctg ctgtggcagc gtgccggcgc gctgatggcg gcgctgccca
     7141 gcgaccacgc gcccggcgat ctcaatcaag gcctcatgga gctcggcgcc acgctgtgct
     7201 cgccgacgtc gccgcggtgc ctgctctgcc cgctcgccgc ccagtgcgaa gccgcgcgca
     7261 ccggccgcca ggacgagctg ccggtcgtcg cggcacggaa gaaggaaagc gagctgccga
     7321 tactcgcgcg cgccgcgctg tggatggcgg atggtgatcg cgtcgtgctc gcgcgccgtg
     7381 cccccgacgg tctgttcggc gggttgtggg agctgccgca gggcgcgtct cgaatagaga
     7441 tcaccaaggc tctcggcgtt acagccgatg cgaccgcggt cgcgtatcac gaccaaacgc
     7501 tgagccaccg ccgcctgcgc atcgcagtgt tccgtgcgag cgtgccgcgc cggcttgtcg
     7561 caaccgcggt tccgggctac gacgctatat cgctcgtcga gcgcgcaact gccaaagcac
     7621 tcggcattgc tgccgccact gctgcgatac tctcgaagta cgaggatagt ccatggagct
     7681 cgatcccaaa gcgctcgctc tcttcaccga gggctacgaa aagatcatcg aaggcctcaa
     7741 ccacctcggg ttcgacacga gcgacgacaa cttcgtcgac acggcgaagc gcgcggcgaa
     7801 agggctccac gagctcgtcc acgaccagaa gcaagtaagg cccgccgtcg aggcgctgct
     7861 cgcgaagacg tttccggcga agtacaccga gatggtgatc agcaagcaca acaccgcgtt
     7921 cggtgtgtgc ccgcaccacc tgctgcccgt cgtctatcgc atctcgatgg cgtatatccc
     7981 gaccgagaag gtgctcgggc tctcgaagct cacgcggctc gcgcgcctca ttgcccgcgg
     8041 cccacgcctg caggaggacc tcacccacga gctcgccgac atcctgcacg aggatctggc
     8101 gagccagggg tcggcggtct acatcgaagg tctgcacatg tgcatggcgg cgcgcggcgt
     8161 cggtgcacac gaagcgcggc tcgtaacgag cggagttcgc ggcgtgttcc tggagctggc
     8221 gacgcgggaa gagttcatca agctcgtcat cgcaccgcag acgacggtgc tctgatcacg
     8281 gtatcttcgc cgtacggatg cgctgcgcgt tgttgcatgg gtttgcggga gatccggcgg
     8341 cctgggacga ggtgatcgac gcctggtcgc tccgtgaggc gccgatcgca gtcgctctgc
     8401 ccggacacgg cggcggaccc gtcctcgata cctgggagga caacctgaac gtcgtcgcag
     8461 gcgcagttgt cggctgcgat gtcgtcgtcg gctactcgct cggcgcgcgc gtcgcgctcg
     8521 gcctcgtcgc gggtggtcgt gcagcacatg gcgtgttcat cggcgtcaac cctggcatca
     8581 acgacagcga gcgcgcacag cgccgcgagt tcgacgccgc atggacgcgc ttgctccgct
     8641 cgagcggcat cgaagcgttc catgaagcgt ggatgcagca accgctgttc gcgacgcaaa
     8701 gccgggtcca gaccgacaag ctcgagcgac ggcgccagcg ccggctcaag ctcgatgccg
     8761 aacagctcgc gcgcagcctc gaggtgatgg gcatcgcgaa catgcccgac tactggggcg
     8821 cgatcccagc gcaccgcgat cggatcgcac tgatcgcagg cgccgacgat ccgaagtacg
     8881 tcgcaatctc gtcgggcctg ccgtgcgcgt cg
//
LOCUS       DASUFX010000317        10493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1716627, whole genome shotgun sequence.
ACCESSION   DASUFX010000317 DASUFX010000000
VERSION     DASUFX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716627"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFV99_12540"
     CDS             <1..697
                     /locus_tag="VFV99_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5060186.1"
                     /translation="ATSVPVDPYAEPAPPAAAGEGGVRVAAAPSEPAPAPPAPIVDSG
                     TPEDPYGGDPGIRAFAEAVGATPAPAPAAPAAVPAPVPAPKPAQVKTKAKAKAAPAPD
                     AAAALAAAATGDATVEVPAAAPPPGAPPPGAIVLARADLDAALANFSKLAASFHATFT
                     AEGLRMDTINDGTLLAKAGLHKGDVVTAVDGRPLRSLDDAANLYARSSTVRSATVQVL
                     RGGKPTTLRVAIQ"
     gene            749..1372
                     /locus_tag="VFV99_12545"
     CDS             749..1372
                     /locus_tag="VFV99_12545"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5060187.1"
                     /translation="MGAVRLELRLNSQSEWIKIFDPRDWTVFVPTDEPIENGTQLRVD
                     LDVGGWVVQLRGAVVGTREDPNGIVVALSGTEREKINYLNGFVRGGLLNLRRKNRLPI
                     KLQVTYGAIEGPAKTFTKDINEEGLFLFTDKPLPETSQVHMLVTVPGKSQPLSLMGKV
                     SHTILAQDEEPPGMGIVFDLDEAQREALVAVIKELEELLEKNQLPEE"
     gene            1552..2376
                     /locus_tag="VFV99_12550"
     CDS             1552..2376
                     /locus_tag="VFV99_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060188.1"
                     /translation="MSSADRVVRAGHGQLIATPTTIALIAGRDSGHLAVPLRRPFTLG
                     TLRLELIPSGRTFGAAALHVDTGMRTVLYAGAIRTTGASEAADVRAADAIVVGAPIGE
                     SQHRVPKLDDVVDQLSTWVRKQLAAAKVPVLVVDTALDGVEVASKLVAGGIAVTATKS
                     VRDAAGVISGAPALAAPGKPPSVIIRAETDRVRVAAATAANALVSVRAVEPEDGWHAT
                     FAWPFAASRDQLLGWIEQTRAKHVFVTGACAETIVAALGAKGHVLGPPQQMALFSS"
     gene            2373..4526
                     /locus_tag="VFV99_12555"
     CDS             2373..4526
                     /locus_tag="VFV99_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5060189.1"
                     /translation="MRSVVIANRRDGGWWWRLPLVALIWLLLATPIIGGSVVVFTLRS
                     WARDVPEVPDLAAWRADAHQTSLIVAADGSHLAELPFRDEHVVGHRTLVTLDALPPQL
                     IEAVLAAEDVRFFSHHGVDYRAVTRAAWANYKAERVVEGASTITQQVARNLLPVEIGK
                     ERSARRKVREALLARRIEQRWTKREILEVYLSYVFLGQGAYGMAAGANAYFDLPVSQL
                     DLAQVALLAGLIQAPSRLDPFHHPDAARDRRNEILARMARAKLIDEPTRARAAAAPIE
                     VRRPHPSYGTRVAWYTEAVRQLVDGAFPDESRRGGLVIETAALPALGTQLQADVVAHA
                     ESWKHDKHVPQVGALLWDYRTGYVEALVGGREWGTDKFDRMMQSCRQPGSAWKPLVYG
                     AALESGAITPGTALRDAPIAEYDEATNTHWKPKSGNQFRGVVLAQDAFAASLNAPAID
                     VFDRVGADNVIRFARRLGITSDLAEVRPMALGASCVKPIELARVYAVIARRGWAIAPR
                     LAVRVRRGDTVLFDATVPEDPWLDGARRYDRIAATAGLDAAERNQATGSQLIQEPIAF
                     QLIDMMRAVVERGTSTAARSLGRDAAGKTGTTNDNTDAWFIGYTGRLLGAVWIGFDDP
                     ATKLGKDGDGAHAALPLWLRAIRAAEADRPKLALPGDAPAGMQRATIDRESGLLAAPG
                     APGLTLWFRDGTAPTEISGNPGTSPTDFGRSSREF"
     gene            4550..5104
                     /locus_tag="VFV99_12560"
     CDS             4550..5104
                     /locus_tag="VFV99_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060190.1"
                     /translation="MQKTPLIALVLFAACGDDPVNYSAPVGIELKAKSGDVANDAITE
                     DKGITTESSNPYGAFVTEAKNRIGHDPSNIVLDTLTLTLGGQSTGVTVLEQVVTGDVV
                     VKFLTNDTNNTFVVGHFASPTGPGPVDGDESFDMAADVGDQDIAKILGGSFHVVISAT
                     PATDFATKGAEASLELKFTFSAFE"
     gene            5147..5614
                     /locus_tag="VFV99_12565"
     CDS             5147..5614
                     /locus_tag="VFV99_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5060191.1"
                     /translation="MFGIGGSEILVILVVALLFLGPDKLPDAAKTISKGIRDLKKQSR
                     ALQQTIESDEKIGGAIRDLKSALRGEEAPRPVRIQPQAKPAELETPADEPVAAIEEAK
                     PSLTLPATAGEPDVDAPAEDHDDDIGSLIKPASGTVAKSDVQPAGDEPKQKHG"
     gene            5607..6503
                     /gene="tatC"
                     /locus_tag="VFV99_12570"
     CDS             5607..6503
                     /gene="tatC"
                     /locus_tag="VFV99_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5060192.1"
                     /translation="MAKPARATTDEGESESDAPDELDGGRMPFLKHLAELRDRVRNAA
                     IAFIVAFLACWYFAKDIFAWLRAPLFDIWAQHCDVKEDPSCIGHGLLEAGKSWGPPKM
                     IYTGLTQPFWVDMSVAMWAGIFVASPFIFHQIWKFIAPGLYKRERKVGVVFAVSSAIF
                     FVTGALFCFYFALPKLYNFLLSYNTRNLLSLPNIQEYLDLTRDSMLAFGAIFELPLLI
                     YFLAMVGLVTHRSLWKFSRWFMVLAFIIGAILTPGPDIVSQFMMALPMIALYNISILL
                     AWRVTAKREKAAAEQRAREDAG"
     gene            complement(6500..7651)
                     /locus_tag="VFV99_12575"
     CDS             complement(6500..7651)
                     /locus_tag="VFV99_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060193.1"
                     /translation="MAFHVFVEGATDATPAGVARLAEAIAKHYGLGAADLHARLQKGR
                     FRVKGNTDRETAEKYRRDLEILGARCVIEEATAQNSQKNTPLPFPAVRPATPVAGTPV
                     LPPKPAAAKPATPPSGTPQYQSGLSAAFSGETPAANLGALAGDGLSFSLSSVDGADEQ
                     PKGDVSFAPPESALPASIGPAPEKPKAKAKAEKPKDEPLDMFAPPDQQGEEFKVDIAS
                     DEKEQTAKKRPSTPVPDESPRASSDKLARPQSEPAARKSQPSLQQPSEPAAVVKSSKL
                     GPLADPRARFAAGVLLAVLIGFVPAHLVASMREKSAYKEIDNKVLAAQQLADTAEVYA
                     TLDRMRTDQLEAKKGEQRNAAIIALAIWALVGGGIAFAWFKKVSWDNFD"
     gene            8110..8814
                     /locus_tag="VFV99_12580"
                     /pseudo
     CDS             8110..8814
                     /locus_tag="VFV99_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(8811..>10493)
                     /locus_tag="VFV99_12585"
     CDS             complement(8811..>10493)
                     /locus_tag="VFV99_12585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060194.1"
                     /translation="GAPPFTGNRVFDILSKQVTEIPAPLPTRRPGVPLWMEAAVAKML
                     AKDPDNRFATTTRLVEALRRGIDTGEVMEDEIARRRESIPPPSVSRVMQKMGIPAPVD
                     TPVLGKETMKGHLEPIPPAQPALPPQPQQVVNKQTMKGHIEGPLPQLPVETPSEERRR
                     TGTPQVGVPVVPEVQAPVTGLIEPRAKTPTAPPPEPMRPKTPSGPVEALPAGRKRQSN
                     AAGPSDSAAWFEEGESASEEADNIRSRKLISPSTTDMSLYDETMMQRRRWPLVAGVLV
                     ALALVGGTIAFALTRDGDTPKKTTAVAEPSPPPPTGDVPPSEIIAPPQPAPAPIAIQP
                     VVAAKAQPATKDAKKDTSSTTPKKTGKTGNAKIDSDDTASRRPGGAWFPTTTTPANTP
                     TTTVVTPTTTTPTPTGVSGVDGPQDPYGTSSDTPPPDGAGADKKAEFYANVGQQQLGS
                     GDTAGAAASFKKALELDPKHVGATIGMGEIALRQGLFGDAIAHLKKAAKLAPKSSRVF
                     TLLGEAYLNSGNNSVAADNFKRALQLDPDNARAREGFDEASAKVPPPEDDAP"
BASE COUNT         1697 a         3499 c         3499 g         1798 t
ORIGIN      
        1 ggcgacgagc gtaccggtcg atccgtacgc cgagcccgcc ccgccggcag ccgctgggga
       61 aggtggagtg cgcgtggctg ccgcgccgag cgagccggcc ccggccccgc cggccccgat
      121 cgtcgatagc ggcaccccgg aggaccccta cggcggcgac cccggtatcc gtgcgtttgc
      181 cgaggcggtc ggcgcgacgc ccgcccctgc gcctgcggcg cccgccgcgg tacccgcccc
      241 ggtgcccgcc ccaaagccgg cgcaggtgaa gaccaaggcg aaggcgaaag cggccccggc
      301 ccccgacgca gctgctgcgc tggcggcagc cgccacgggt gacgcgacgg tcgaggtccc
      361 ggccgcagcg cccccgccgg gcgcgccccc gcctggtgcg atcgtgctgg cccgcgccga
      421 cctcgatgcg gcgctcgcca acttcagcaa gctcgcggcc tcgttccacg ccacgttcac
      481 ggccgagggc ctgcgcatgg acacgatcaa cgacggcacc ctgctggcga aggccggcct
      541 gcacaagggc gacgtggtga cggcggtcga tggccgcccg ctgcgctcgc tcgatgatgc
      601 cgcgaacctc tacgcgcgct cgtcgacggt gcgctcggcc accgtgcagg tgctgcgcgg
      661 cggcaagccc accacgctgc gcgtcgcaat tcagtaacca cgcgactgcc gatggcggca
      721 gcgcgccgcg ctggtttacg atggggccgt gggagcggtt cggctcgagc tacggctcaa
      781 cagccagtct gagtggatca agatcttcga tccgcgcgac tggaccgtct tcgtgccgac
      841 cgacgagccg atcgaaaacg gtacccagct ccgcgtcgat ctcgatgtcg ggggctgggt
      901 cgttcaattg cgcggtgccg tcgtcggcac gcgtgaagat ccgaacggca tcgtcgtcgc
      961 gctgtcgggt accgagcgcg agaagatcaa ttacctgaac ggcttcgtac gcggcggtct
     1021 gctcaacctg cgccgcaaga atcggcttcc gatcaagctg caggtcacct acggcgcgat
     1081 cgaaggcccc gcgaagacgt tcacgaagga catcaacgaa gaaggcctgt tcttgttcac
     1141 cgacaagccg ctgcctgaaa cgtcgcaggt tcacatgctc gtgaccgtgc cgggcaagtc
     1201 gcagccgctg tcgctgatgg gcaaggtctc gcacacgatc ctcgcacagg acgaagagcc
     1261 gcccggcatg ggaatcgtgt tcgatctcga tgaagcgcaa cgtgaggcgc tcgtcgctgt
     1321 gatcaaagag ctcgaagagc tgctcgagaa gaatcagctg ccagaagagt agatcggcag
     1381 cagatcgccg ggccgctgct aagttgcctt gctgcctggc ggatgtcacc gtgtgccgtg
     1441 gcacgcagac cacgcgcggc tccagttccg ccggttacct ggcgggatgg cgtgcacctg
     1501 accgggacgc cgatctggtg cgacgctcgg cggcgccgtg acgtttgctt cgtgtcgagc
     1561 gcggaccgcg tcgtgcgcgc gggccacggc cagctcatcg cgacgcctac gacgatcgcg
     1621 ctgatcgccg gacgcgatag tggtcatctc gcggtcccac tgcgccggcc gttcacgctc
     1681 ggcacgctgc gtctcgagct gattccatcc ggccgcacgt tcggcgccgc ggcactgcat
     1741 gtcgacaccg gcatgcgcac ggtgctgtac gccggtgcga ttcgcacgac cggagcgagc
     1801 gaggccgctg acgtgcgagc cgcggatgcg atcgtggtag gcgcgccgat cggcgagtcg
     1861 cagcatcgcg tgcccaagct cgacgacgtt gtcgatcagc tgagcacgtg ggtacgcaag
     1921 caactcgcag cggcgaaggt gccggtgctc gtcgtcgata ccgcgctcga tggcgtcgag
     1981 gtcgctagca agctcgtcgc tggcggaatc gcggtgactg cgacgaagtc cgtgcgcgat
     2041 gcagcgggcg tgatcagcgg cgcacctgcg ctggccgcgc ccggtaaacc accgtcggtg
     2101 atcatccgtg ccgagaccga tcgcgtgcgc gtcgcggcgg ccactgccgc gaatgcgctc
     2161 gtatcggtgc gagccgtcga gccggaggac ggttggcacg caacgttcgc gtggccattc
     2221 gcggcgagcc gcgaccagct gctcggctgg atcgagcaaa cacgcgccaa gcatgtgttt
     2281 gtcaccggcg catgtgccga aaccatcgtc gccgcgcttg gtgcgaaagg tcacgtgctc
     2341 ggaccgccgc aacagatggc attgttctcg tcatgaggag tgtcgtcatc gccaatcgcc
     2401 gcgacggcgg ctggtggtgg cgactgccgc tcgtcgcgct gatctggctc ttgctcgcga
     2461 caccgatcat cggcggcagc gtcgtcgtgt tcacgctgcg cagctgggcg cgcgacgtgc
     2521 cggaggtccc cgacctcgcg gcgtggcgcg cggacgcgca ccagacgtcg ctgatcgtgg
     2581 ccgccgacgg ctcgcacctc gccgagctgc cgtttcgcga cgagcacgtc gtcggccatc
     2641 gcacgctcgt cacgctcgac gcgctgccgc cgcagttgat cgaggccgtg ctcgcggccg
     2701 aggacgtgcg gttcttctcg catcacggcg tcgattaccg cgcagtgacg cgtgccgcgt
     2761 gggcgaacta caaggccgag cgcgtcgtcg aaggcgcatc gacgatcacg caacaggttg
     2821 cacggaatct gttgccggtc gagatcggca aggagcgcag cgcgcgccgc aaggtgcgcg
     2881 aggcgttgct cgcgcgtcgc atcgagcagc gctggaccaa gcgcgagatc ctcgaggtgt
     2941 acttgagcta cgtgttcctc ggccaaggcg cgtacggcat ggccgcgggc gcgaacgcgt
     3001 acttcgatct gcctgtcagc cagctcgatc tcgcgcaggt cgcgctcctc gcggggctca
     3061 tccaggcacc gagccggctc gatccgtttc atcatcccga cgccgcacgc gatcgccgca
     3121 acgagatcct cgctcgcatg gcgcgtgcga agctgatcga cgagccgacg cgtgcgcgcg
     3181 ctgcggccgc gccaatcgaa gtgcgccgtc cgcatccgag ctacggcacg cgcgtcgcct
     3241 ggtacaccga ggccgtgcgc cagctcgtcg acggcgcgtt tcccgacgag agtagacgcg
     3301 gtggcctcgt gatcgaaacg gccgcactgc ccgcgctcgg cacgcagctg caagcagacg
     3361 tcgtcgctca tgccgagagc tggaagcacg acaagcacgt gccgcaggtc ggcgcgctgc
     3421 tctgggacta ccgcaccggc tatgtcgaag cgctcgtcgg tggtcgtgag tggggcaccg
     3481 acaagttcga tcgcatgatg cagagttgtc gccagccggg ttcggcgtgg aagccgctcg
     3541 tctacggcgc ggcgctcgag agtggcgcga tcacaccggg caccgcgctg cgcgatgcgc
     3601 cgatcgccga atacgacgag gcgacgaaca cgcactggaa accgaaatcc ggaaatcagt
     3661 ttcgcggcgt cgtgctcgcg caggacgcgt tcgctgcgtc gctcaacgca cccgcaatcg
     3721 acgtattcga tcgcgtcggc gccgacaacg tgatccgctt cgcgcgcagg ctcggcatca
     3781 cgagcgacct cgcggaagtg cggccgatgg cgctcggtgc atcgtgcgtg aagccgatcg
     3841 agctcgcgcg cgtgtacgcg gtgatcgcgc ggcgcggctg ggcgattgca ccacggctcg
     3901 cggtgcgcgt gcggcgtggc gacaccgtgt tgttcgacgc gaccgtgccc gaggatccgt
     3961 ggctcgacgg cgcgcgtcgc tacgatcgaa tcgcggcgac ggccggtctc gacgcagcag
     4021 aacgcaatca agccactggc agccagttga tccaggagcc gatcgcattc cagctgatcg
     4081 acatgatgcg tgcggttgtc gagcgcggca cgtcgacggc cgcgcgcagt ctcggtcgcg
     4141 atgcggccgg caagaccggc acgacgaacg acaacaccga cgcgtggttc atcggctaca
     4201 ccggccgctt gctcggcgcg gtgtggatcg gttttgacga tccagcgacg aagctcggca
     4261 aagatggcga tggcgcgcac gctgcgttgc cgctgtggct gcgtgcgata cgcgcagcag
     4321 aagctgatcg tccaaagctc gcgttgcccg gtgacgctcc cgctggcatg caacgtgcga
     4381 cgatcgatcg cgagagtggc ctacttgccg caccaggtgc tccaggtctc acgctgtggt
     4441 ttagagacgg cactgcaccg acggaaatca gcggcaatcc aggtacatca ccgacggact
     4501 tcggacgttc gtcacgcgag ttctagcgag aagcgcgtta gctttcggga tgcagaagac
     4561 tcctcttatt gcactcgttc tgttcgctgc gtgtggtgat gacccggtca actactcggc
     4621 gccggtcggc atcgagctca aggcgaagtc gggtgacgtc gcgaacgacg cgatcaccga
     4681 ggacaagggc atcacaacag agagcagtaa tccgtacggg gcgttcgtca ccgaagcgaa
     4741 gaaccgcatc ggacacgacc cgagcaacat cgtgctcgac acactgacac tgacgctcgg
     4801 cgggcaatcg accggtgtca cggtgctcga acaggtcgtg accggcgacg tggtcgtaaa
     4861 gttcctgacc aacgatacga acaacacgtt cgtggtcggg cattttgcca gcccgaccgg
     4921 tccaggcccg gtcgacggcg acgaaagctt cgatatggca gcggacgttg gcgatcagga
     4981 catcgccaaa atcctcggcg gcagcttcca cgtcgtcatc agcgcaacgc cggccacgga
     5041 ttttgctacc aagggggcag aagcctcgct ggaactgaag tttacattca gcgcgtttga
     5101 atgacgcagg cgccctcggg cgacccatgg tgctatgctg cttccgatgt ttggcatcgg
     5161 cggcagcgag atcctcgtga tcttggtagt cgcgctccta tttttggggc ccgacaagct
     5221 gcctgatgct gccaagacga tcagtaaggg catccgcgat ctgaagaagc agtcgcgcgc
     5281 gctccagcag acgatcgaaa gcgacgagaa gatcggcggc gcgattcgcg acctcaagag
     5341 cgcgttgcgc ggcgaggaag cacctcgtcc ggtccgcatc cagccgcagg ccaaacccgc
     5401 ggagctcgag acgccagcgg acgagccggt ggcagccatc gaggaggcga agccgtctct
     5461 gacgctgcct gccaccgctg gtgagcccga tgtcgacgcg cccgctgagg accacgacga
     5521 cgacatcggc tcgttgatca agccggcgag cggcacggtt gcgaagtccg acgtccaacc
     5581 tgccggcgac gagcccaagc agaagcatgg ctaagccggc acgcgcaacg acggacgaag
     5641 gcgagtccga gtccgacgct cccgacgagc tggacggcgg ccgcatgccg tttctcaagc
     5701 acctcgcgga gctccgcgat cgcgtgcgca acgcggcgat cgcgttcatc gtcgcgttcc
     5761 tcgcgtgctg gtatttcgcg aaggacatct tcgcgtggtt acgcgcgccg ctgttcgaca
     5821 tctgggccca gcactgcgat gtcaaagagg acccgagctg catcggtcac ggcctgctcg
     5881 aggccggcaa gagctggggc ccgccgaaga tgatctacac gggcctgacc caaccgttct
     5941 gggtcgacat gtccgtcgcc atgtgggcgg gcatcttcgt ggcgtcgccg ttcatcttcc
     6001 accagatctg gaagttcatc gcgcccggcc tctacaagcg cgagcgcaag gtcggcgtcg
     6061 tgttcgcggt ctcgtcggcg atcttcttcg tcaccggtgc gctgttctgc ttctacttcg
     6121 cgctgccgaa gctctacaac ttcctgctct cgtacaacac gcgaaatctg ctgtcgctgc
     6181 cgaacatcca ggagtacctg gatctcacgc gcgactcgat gctcgcgttc ggcgcgatct
     6241 tcgagctgcc gctgctcatc tacttcctcg cgatggtcgg gctcgtcacg caccgctcgc
     6301 tgtggaagtt cagccggtgg ttcatggtgc tcgcgttcat catcggcgcg atcctgacgc
     6361 ccggccccga catcgtgtcg cagttcatga tggcgctgcc gatgatcgcg ctctacaaca
     6421 tctcgatcct gctcgcgtgg cgcgtcacgg ccaaacgcga gaaggccgcc gccgagcagc
     6481 gcgcccgcga agacgccggc taatcaaagt tgtcccacga gactttcttg aaccaggcga
     6541 acgcgatgcc gccgcccacg agcgcccaga tcgcgagcgc gatgatcgcg gcgttgcgct
     6601 gctcgccctt cttcgcctcg agctgatcgg tgcgcatgcg atcgagcgtc gcgtagacct
     6661 ctgccgtatc ggcgagctgc tgcgcggcga gcaccttgtt gtcgatctct ttgtacgccg
     6721 acttctctcg catcgacgcg acgaggtgcg ccggcacgaa gccgatcagc accgcgagca
     6781 gcacaccggc ggcaaaccgc gcacgcggat cggcgagtgg gccgagcttg ctcgacttca
     6841 cgaccgcagc gggctcgctc ggctgctgca gcgacggttg tgatttgcgc gccgcgggct
     6901 cggattgcgg gcgtgccagc ttgtccgacg acgcgcgcgg cgattcatcc ggcaccggcg
     6961 tgctcggccg cttcttcgcg gtctgctctt tctcgtcgga cgcgatgtcg accttgaatt
     7021 cttcgccctg ctgatcgggc ggcgcgaaca tgtcgagcgg ttcgtccttc ggcttctcgg
     7081 ccttcgcttt cgccttaggt ttctccggcg ccgggccgat gctcgctggc aacgcactct
     7141 ccggtggcgc aaacgaaacg tcgcctttgg gctgctcgtc ggcgccatcc accgacgaga
     7201 gcgagaacga aaggccgtcg cccgcgagcg caccgagatt cgctgccggc gtctcacccg
     7261 agaaggcggc agacaaaccg ctctggtact gcggcgtgcc cgatggtggc gtagccggct
     7321 tcgctgctgc gggttttgga ggcagcaccg gcgtacccgc gacgggtgtg gcaggacgga
     7381 cagcgggaaa tggcaggggc gtgtttttct gcgagttctg cgcagtcgcc tcttcgatca
     7441 cgcagcgcgc cccgagaatc tcgaggtcgc ggcgatattt ctccgcggtt tctcgatcgg
     7501 tgttgccctt gacgcgaaag cggccttttt gcaggcgcgc atgtaggtca gcggccccca
     7561 ggccgtagtg cttggcgatc gcctcggcga gacgcgcgac gcccgcaggc gtggcgtccg
     7621 tcgctccctc gacgaacacg tgaaatgcca tctaggccca tcatcgcata ggacgagggg
     7681 agatctctcc cgcgatcaca aagttgcgca gcgcgatcaa cgaccctcag gatgaatcat
     7741 gcacgtcggc atcaagcgtc ctaagatctc atggtctagc cgccgcatcc gcctcggagt
     7801 cgcacaccat ggcgctccct cggcgtggac cgcaccggtg aaggacgtcg cgcaagatcg
     7861 caagcacccg aagaagggac gacgctagcc agtttcagct acgctctccg cgtgcagtgg
     7921 gtgctgctcg cggtcgtcgt ggtctcggcc tgccgggccg atgatccctt cgtctgcgca
     7981 agcgacggcg agtgtcgtcg caacggcatg accggtacgt gtgaagcgag cagcaactgc
     8041 agcttcccgg acatcgattg cccgacgggt cgtcgttacg acgagctcgc gacgcaatcc
     8101 ggtcagtgcg tgctcgacga cgatcaggac atgatcgccg acgacggcga caactgtccg
     8161 acgatcccga atcccgacca gttcgacgag gacaaggacg gcgtcggcga catctgcgat
     8221 ccgtgcccgg tattcgcgat gaaccgcgac gacgatggtg acggcgtcgg caatctctgc
     8281 gatccgcgcc ccgatacacc gggcgatcag atcgtgttct tcgaaggctt ccacacgccg
     8341 ctgcccgtgg agtggagcgt gaccggtgcc gccgtgatcg agaacgatgg cctcacgctc
     8401 cagaatatga gccaagcgtc gctgccgatc gcaccgagcg gaaaccaaac gttgatcgcg
     8461 agcgcgaaga tgccatcgac ctcgcttggc acctggttcg tcggcttgcc gttccaggac
     8521 aacgtcggcg gcgcctactg ccagctgaca accgagaagc tgcggctcta ttccgttgcc
     8581 accgtcgaca cgttgctcgg cgaggccgcg ttcgccgcga agatcaacac ggcgtacacg
     8641 ctcgcgatcc agaagacggg ccagcagacg acgtgcagcg cgcgaggaac accaaccgcg
     8701 ccaccggttt cggtgagcgg cacgccgatg acgacgccga caatgccgac gatcaagatc
     8761 ggctgcgatg ccagcaccca cttcgcgtgg gtgatggtcg tcgcgaacaa ctagggtgcg
     8821 tcgtcttcgg gcggcggcac tttcgccgac gcctcgtcga agccttcgcg tgcgcgcgcg
     8881 ttgtcgggat cgagctggag cgctctcttg aagttgtcgg cagcgacgct gttgttgccg
     8941 ctgttgaggt acgcctcgcc gagcagtgtg aacacgcgcg agctcttcgg tgcgagcttt
     9001 gccgctttct tgaggtgcgc gatcgcgtcg ccgaacaggc cctggcgcag cgcgatctcg
     9061 cccatgccga tcgtcgcacc gacatgcttc ggatcgagct cgagcgcctt cttgaagctc
     9121 gccgcagcgc ctgccgtgtc accactgcca agctgctgct ggccgacgtt cgcgtagaat
     9181 tcggccttct tgtcggcacc cgcgccatcg ggtggcggcg tgtcgctgct cgtgccgtac
     9241 ggatcctgcg gaccgtcgac accgctgacg cccgtcggcg tcggcgtcgt ggtggtcggc
     9301 gtcacgaccg tcgtcgtcgg tgtattcgca ggcgtggtgg tcgtcgggaa ccacgcgcca
     9361 ccggggcgac gcgacgcggt gtcatcgctg tcgatcttcg cgttgccagt cttgcccgtt
     9421 ttcttgggcg tcgtcgacga cgtgtccttc ttcgcgtcct tcgtcgcggg ctgcgccttc
     9481 gccgcaacga ccggctggat cgcgatcggt gccggtgcgg gttgtggcgg cgcgatgatc
     9541 tcgctcggcg gcacgtcacc ggtcggcggt ggcggcgacg gctcggcgac cgcagtcgtt
     9601 ttcttcggcg tgtcaccgtc acgcgtgagc gcaaacgcga tcgtgccacc gacgagcgcg
     9661 agcgcgacga gcacaccggc cacgagtggc cagcgacggc gctgcatcat cgtctcgtcg
     9721 tacagcgaca tgtccgtcgt gctcggcgag atcagcttgc gcgagcggat gttgtcggct
     9781 tcttcggagg cgctctcgcc ttcttcgaac cacgcagcgc tatcgctcgg acctgcggcg
     9841 ttgctctgcc gcttgcgacc agccggcagc gcttcgaccg gaccgctcgg cgtcttcggg
     9901 cgcatcggtt ccggcggcgg cgccgtcggc gtcttggcgc gaggctcgat gaggcccgtg
     9961 acgggcgctt gcacctcggg gacgacgggc acaccgactt gcggcgttcc ggtgcggcgg
    10021 cgctcttccg acggcgtctc gaccggaagc tgcggcagcg ggccttcgat gtggcccttc
    10081 atcgtctgct tgttgacgac ttgctgcggc tgcggcggca gcgcgggctg cgcaggcgga
    10141 atcggctcga gatgaccttt catcgtctct ttgccgagca ccggcgtatc gaccggcgcg
    10201 ggaatgccca tcttctgcat cacgcgcgac acgctcggcg gcgggatgct ctcgcggcga
    10261 cgcgcgatct cgtcctccat cacctcgccg gtgtcgatgc cgcgacggag cgcctcgaca
    10321 agacgtgtcg tcgtcgcaaa tcgattgtct ggatccttcg cgagcatctt cgcgacggcg
    10381 gcttccatcc acagcggcac gcccggacga cgcgtcggca gcggcgcggg aatctcggtg
    10441 acctgcttcg acaggatgtc gaatacgcga ttgccggtga acggcggcgc acc
//
LOCUS       DASUFX010000318        26403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1437048, whole genome shotgun sequence.
ACCESSION   DASUFX010000318 DASUFX010000000
VERSION     DASUFX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 26403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26403
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437048"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFV99_12590"
     CDS             <1..180
                     /locus_tag="VFV99_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5060195.1"
                     /translation="AMNGDQLSPGQYAVSTSNRNFEGRQGRGGRTFLASPQTAAAAAI
                     TGRITDVRTLLGGAR"
     gene            177..743
                     /gene="leuD"
                     /locus_tag="VFV99_12595"
     CDS             177..743
                     /gene="leuD"
                     /locus_tag="VFV99_12595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5060196.1"
                     /translation="MNAFTTLTARAVVLAADDVDTDPIIPARFLKGTSKVGLAKHLFA
                     DWRTQPGFPLLAAADGTDAQILVTGVNFGCGSSREHAPWALMDYGFRAIIARSFADIF
                     KQNATKNGLLPVELPGAVCLQIEQLVNRNSSTQLRIDLATQRVTLPDGNAFPFEIDPF
                     AKHCLTNGIDELGYILSFSERIAAHEQR"
     gene            740..2329
                     /gene="cimA"
                     /locus_tag="VFV99_12600"
     CDS             740..2329
                     /gene="cimA"
                     /locus_tag="VFV99_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX5060197.1"
                     /translation="MTIAIYDTTLRDGTQREGISLACSDKLRIAEQLDALGVAFVEGG
                     WPGSNPKDVELFRRARDIEWKHATLTAFGATRRAGIAPEDDANLAALIESGARACTIF
                     GKTSLLHVREVLRTTPDENLAMIEDSVRYLVTLGRRVIYDAEHFFDGADEDHAYALST
                     LLAAVRGGAEAVVLCDTNGGTLPWQITQRVSAVVAALPVAVGIHAHDDSGCGVANSLA
                     AVRAGATQVQGTLNGYGERCGNANLTTIIPALELKLGYRCLRPGALAELGRVARFAAE
                     TANLAFDEHAPYMGRSAFAHKGGVHVAAIRRSPRSYEHVDPTLVGNRTRFVVSELSGR
                     ANVRAIAEAHEVDVTSDAEANVLETVKEREARGFAFESAEASVALMMRRAQPDYTAPF
                     ELLDYKILAGKQEPSADAAVKVRVRGELVHTAADGNGPVSALDAALRKALIAAFPEVE
                     HIHLEDYKVRIIDGRAGTAATTRVLIDHGNGHDRWTTVGASPSILEASRLALVDGIEH
                     GIWLARGKEESDEGHNRLAAG"
     gene            2298..3293
                     /gene="leuB"
                     /locus_tag="VFV99_12605"
     CDS             2298..3293
                     /gene="leuB"
                     /locus_tag="VFV99_12605"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5060198.1"
                     /translation="MKATIVLLPGDGIGPEVVHEARAVLEVIAARYGHSFSFAEHLIG
                     GAAIDATCEALPGATLAACERADAVLLGAVGGPKWDNPKAKVRPEQGLLAIRKALGLY
                     ANLRPVGVHPALAAVSPLKSDRIEGVDILFIRELTGGLYFGWPRFREEIGDRRRAVDT
                     LEYTEDEIRRVVRLAFRLARTRKKRVTSVDKANVLESSRLWREVAVEVSAEFPDVALE
                     HQLVDSCAMRLVTNARSFDVIVTENMFGDILTDEAAVLAGSLGLLPSASLGAGTRGLY
                     EPIHGSAPDIAGKGIANPIGTILSAAMLLRHSLGLAEEADAVERAVREAIADGAR"
     gene            3518..4735
                     /locus_tag="VFV99_12610"
     CDS             3518..4735
                     /locus_tag="VFV99_12610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5060199.1"
                     /translation="MTSSIGARSETWMRRTTLLACFLHFDFSFMLWVLIGALGIPLAE
                     AAHLSPAEKGLVVAVPILAGSLMRIVVGLLTDRFGGKRVGVMVLAFLFLPLALAWQLE
                     PSLPALVVIGAMLGVAGASFAVALPLASRWFPPERQGLAMGIAAAGNSGTVVTNLVAP
                     RLVLAIGLGATFGVAMIALGFVLVAFSLLAYEPARRAVRPRFPSFRDADLRWLCLFYA
                     ITFGGYVGLSSFLPLFMRDAYGVTPIHAGYITAALACCGSFARPIGGIIADRIGGTRM
                     LAILLFPIAAAYGVAASLPSLPMMIGVFAVAMICLGLGNGAVFQIVPQRFVRDLGAVT
                     GLIGAIGGVGGFFLPTLLGSVRGATGSFAVGFLVLATIAGLAAISLRVLVSSRGRWAH
                     AMQPVVATEGVAP"
     gene            4732..5622
                     /locus_tag="VFV99_12615"
     CDS             4732..5622
                     /locus_tag="VFV99_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060200.1"
                     /translation="MRALVTTLVLLAATNADADVVAKPAPFSLGWSLRPAGPVNVVRS
                     DTAIGLFDDGGASGSTVASTFLVAYKVSPELSPLLRFAVIHNDPATGGTATAMSNPLV
                     GAMWAPPIVEAPYKLAFFGGLALPLGEGGGNAANPTMTSTVRSAIAARSSMDNAMFAI
                     NDLTPIVGIDAAYVANGLTLQLEATVFELFRVRGEDVQPDAYKTNFTSGAQAAYFVFD
                     WLSAGAELRYQRWLSTPAAVEMAPATRDTVSAAVGVRGHFAVGGPRWLRPGVSYARGL
                     DDPMTGRNYQIVQIDVPFAF"
     gene            5632..6432
                     /gene="cobA"
                     /locus_tag="VFV99_12620"
     CDS             5632..6432
                     /gene="cobA"
                     /locus_tag="VFV99_12620"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5060201.1"
                     /translation="MKSGKAYLIGAGPGDPKLLTLRAAEVLRQADVVLVDDLVHPDVL
                     SHARTARIIRVGKRSQRRAFPQELATNILIAYVRGGAIVARVKGGDPMLFGRGGEEAL
                     ALAEAGLPFEIVPGVTAALGAAAYAGIPLTHRGTASSVAFVAGHREGDQPARLDVEAD
                     TIVVYMCQRTIHAIARELIAAGRSPMTCIALVRGASWQSQELYTGYLGEAAALEDDWY
                     AVLDPEPPTVAIIGAVASYADQMQWFGRRATPLAMLMASPLRYAAGGR"
     gene            6432..9230
                     /gene="nirB"
                     /locus_tag="VFV99_12625"
     CDS             6432..9230
                     /gene="nirB"
                     /locus_tag="VFV99_12625"
                     /inference="COORDINATES: protein motif:HMM:NF011565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX5060202.1"
                     /translation="MSRLVVIGNGMVSHRFCEKLVEYGLIAHHEIVVFGEEPRPAYDR
                     VHLTEYFGHRSADQLRLGSQAWYAENHIALRVGIRIARIERECRVVVTEAGERIAYDT
                     LVLATGSAPFVPAIPGVDKAGAFVYRTIDDLDAIQGWASRAQRAAVIGGGLLGLEAAR
                     ALVEVGIDTHVLEVAPRLMPRQLDNAGAALLARSIQRLGVTLHLGTQVTEIGGDDAVR
                     WVQLPDRRLHLDMVIISAGIRPRDELAREAGIVCGDRGGVIVDKHMCTSDRHVFAIGE
                     VALHRGTAYGLVGPGYEMADVLAKRIAGHDAAFTGGDQSAKLKLLGTDVASFGNPFAD
                     DPGTRAIAFEDQLRGVYQKLIVTRDGMRLVGGILVGDTSEYGRLLHLTRAKQALNGEV
                     PLFSRGTAPSGELPDDVQVCSCNNVSACTIRERARAGDTIGEIKACTKAGTGCGGCLP
                     LVAELVQVELARSGRATKRVLCQHFAHTRQELFEIVAATQIRTFEELLRAHGTGTGCE
                     ICKPTVASILASIHNEPILDEPHRPLQDSNDRYLANIQKNGTYSIVPRMPAGEVTPEQ
                     LIALGNVAIKYGLYTKITGGQRVDLFGARVDQLPGIWEELIAAGFESGHAYGKAMRTV
                     KSCVGSTWCRYGVQDSTAFAIRIEHRYKGIRAPHKLKSAVSGCVRECAEAQGKDFGLI
                     ATERGWNVYVCGNGGAKPRHADLLASDIDEDTAIRYLDRFLMFYIRTADRLTRTSVWL
                     DKLPGGIEHLRDVVVHDSLGIAGELERQLQHLVDTYECEWAGVVRDSAKRAQFHETRA
                     TVRLPVVDHPLGRQFVRVASLRDVPAEGGIAIQYGHAQVAVFRAGGELYATQNSCPHT
                     QASVLARGIVGDDCGAPKVACPLHKRTFDLRSGACLSQDAPPIRTFPIRVDGDDILVE
                     LPPPDALAACAGEKALAS"
     gene            9230..10798
                     /locus_tag="VFV99_12630"
     CDS             9230..10798
                     /locus_tag="VFV99_12630"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5060203.1"
                     /translation="MLRLPLLDQYLREQHDLTAVERFSRAHDTALDGDRYRELMPATP
                     PGDGQQYAFEVDLDACSGCKACVTACHNLNGLDEGETWRAVGLLHGGTAAAPVKQVVT
                     SACHHCLDPACMNGCPVRAYEKDPVTGIVKHLDDQCIGCQYCTFTCPYEVPQYNAKRG
                     IVRKCDMCSDRLAADEAPACVQSCPNEAIAIRVIDQRQALDDAQGDAFLPGTPSPAIT
                     VPTTIYRTAQPPPRNLLPADFYSVAPSHHHVPLVVMLVLTQLSVGAFVIDAVVTRFIA
                     PHVLQPIHALAALVVGLAALAASVFHLGRPLYAFRAVLGLGTSWMSREIVAFGAFAML
                     AMSYAALSLTGAAPVALQTALGGAVAAIGVIGILCSALIYHVTRKAWWTVSITGFKFF
                     MTAALLGLASTIVTLSSSHTGGDLAPRLSRLVVLVSIVKLLGELVVLVHLRDKRYTEL
                     KRSAVLLVRDLRRWSIPRVAALIAGGILLPLAGEPTLVPAIASFVLLVIGELLERTLF
                     FAAASAPGMPKGVR"
     gene            10795..13014
                     /locus_tag="VFV99_12635"
     CDS             10795..13014
                     /locus_tag="VFV99_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX5060204.1"
                     /translation="MKSLRHLLYQRDGALTHDLARVPAGFGIGQVPVRLQPDSVTSMI
                     CGFCSTGCSLDIHIRDGRAVNITPTADYSVNTGAACPKGWDALSPLGATDRATTPLLR
                     SAAGNLEPVSWPTAIDAFVTRMTAIKAKYGGEALAFLGTGQMPSEELALLGALAKFGI
                     GMVHGDGNTRQCMATAVVAYKQAFGFDAPPYTYADFEESDVLVFVGANPCVAHPIMWA
                     RVCKNPHDPTIIVVDPRRTETAMAATLHLPLRPKSDLVLLYAIANVLVQRDWIDRAFV
                     DAHTNGFAELATHVAPYTLDRASEATGLPPEAIEQVAAHIHCGKRVSFWWTMGVNQSH
                     EGVRVAQALINLALLTGNIGRPGTGANSITGQCNAMGSRLFANTTNLLGGHDFTRADH
                     RDKVAGILGIDSAAISTKNSLAYDQILEAINRGKIRALWIVATNSAHSWINQSDAVDV
                     LRRLDLLVVQDMYATTETARCAHLVLPAAGWGEKEGTFINSERRLGLIKRVAHAPGHA
                     LADFYIFKLIAEAWGCGDMFERWSSPEAVFRLLAEVTRGQPCDITGITGYDDIERLRG
                     VQWPAPAGESIAPGSERRLFADGRFCHPDGKARFVVGEPQAPPEQPDATYPFTLLTGR
                     GSSSQWHTLTRTGKSPVLEKLGTSTPYVELSPVDAAALRISPGQLVVIESRRGRMPAR
                     AFVTHGVQPKHVFVPMHYARTNQLTFPAFDPESRQPAYKSCAVAVRAIREGERVEDL"
     gene            13659..14993
                     /locus_tag="VFV99_12640"
     CDS             13659..14993
                     /locus_tag="VFV99_12640"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX5060205.1"
                     /translation="MIRPPKLPTDPDRKLARRILAQMGENGKPPELGISHVNVGNESY
                     LQVIDAAYVGDLIGVVEGSSFKIVQGTYGAGKTHFLYCVRDLAWRRGLLAAFVTISPK
                     ECPFNRPLAIYKAIANSIELPRGTGGEITRGVDDVIRANLEARIAEEGRDATRAWLDK
                     QLARAQITRHSFRDAVVGFSRAVLDNDEPTARLLGAWLRGEDVSVSEVKAAGVFELPS
                     NENGFGMVRSLVQLVHQLGYEGTVVLLDEAERRLSVDGKPTKTMAETMDHLRELVDLC
                     GRSELPRTLVLYAVTPAFTADVLPQYPALQQRLGAPIQFLSTTNPKAPVIDLEALDLE
                     PRKLLVELGRRLVVVAKRAYEWSPNDKLVERNLDLLASIVAEEQLEVGHRRFFVKLCI
                     RLLDQQRIGGEKKLDDDHIQALVRDEQALQLEEARPDEYGEFLGITFRKKKD"
     gene            complement(14998..17055)
                     /locus_tag="VFV99_12645"
     CDS             complement(14998..17055)
                     /locus_tag="VFV99_12645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060206.1"
                     /translation="MQSCARTNTIAALLAGAADPAAAERLEVHLDECPACRRLVANLG
                     RGLSAIGSARPGSLAHGLPRPGESIARYEIVRVIGVGGMGVVYEAQDTTLARRVALKL
                     LRPDLLEGGSLLAEAQAMARLQHPNIAAVHDAGIANGQLYVCMELVAGSTLRAWVTEQ
                     PRDWRAIVTLFLAAGEGLAYVHRAGLVHLDFKPDNVLVDHDGRVRVTDFGLARIIGAS
                     TSRAIVGTPAYMAPEQRAGAVTDARSDQYSFCVALREALDGAAALRAVHRVIERGLAV
                     HPDDRYPSMAELLAALAAAIRPSRKRGAAVAVVGATMLVTAAVSVLPGTTGTITRIVE
                     RPVAQAVVEYRERVVAAPAVDDVPTPVMVAASSRSESVAPAAARFVPPRRAPALHLDS
                     FMNTVMFANALPNDLATDESADRAAHGGAGYCDDGSPMSCGWWAPSCPAGTVLAIQQG
                     CWTCADEQTCGPLGFPRTCNDGSPLRCTQEPPTCSGHRIAAVRNGCWSCEDPFSCWSW
                     VKQPTQPPPRPPPKDPGSGSGSGHGSGSGSGSGSGSGSGSGSGWGSGSGSGSGWGSGS
                     GSGSGWGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSAMCGNGYCEFGESYASCASD
                     CCDTGSGSNCVPVCGNGYCEAGETHAACASDCCELIGSACAPICGNGVCEPGESPSCS
                     DCN"
     gene            complement(17126..18055)
                     /locus_tag="VFV99_12650"
     CDS             complement(17126..18055)
                     /locus_tag="VFV99_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060207.1"
                     /translation="MIAAVVHPSTMVSSDAVRAFWTALAALHRPASLDVLVPHVTAAW
                     QRARAAHPQLAVDETALMTFAGERIATSEPGAELERRHVEDLYLACGCARGDRAALAT
                     LEDETLPIVMRGLTKIAQSDDARRELIQMLREQMLVARDGAPGIAAYDGRAPLAIWLR
                     VCAARLGMRQAAHDGRNQPLDDEHLDQLAPGVPDPQLAYLQQHYGAQFRAAFREAVTT
                     LTPRERNLLRHSVIDGLSIDQIAAIYHVHRATAARQLKQTRETLIARTREQMRLALGI
                     GETELDSIFRMLVSMADVTLREILRPQPRKTDG"
     gene            complement(18064..18537)
                     /locus_tag="VFV99_12655"
     CDS             complement(18064..18537)
                     /locus_tag="VFV99_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060208.1"
                     /translation="MRVLVMFVAALVAGFVACVDEPDVSGPSATSLSSGDDGSGGSDG
                     SGCGSGSGWGSGSGSGWGSGSGSGWGSGSGSGSGSGSGWGSGSGSGSGSGWGSGSGSG
                     SGSGSGSGWGSGSGSGCGGGSGSGSGSGSGSGSGSGSGSGSGSGSGGGSGSGSGD"
     gene            complement(18617..19492)
                     /gene="rluF"
                     /locus_tag="VFV99_12660"
     CDS             complement(18617..19492)
                     /gene="rluF"
                     /locus_tag="VFV99_12660"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HEX5060209.1"
                     /translation="MLEVRLNKFLSESGACSRREADELIAEGRVTVNGKPAVVGTQVS
                     ETDEVRLDGDRVGQARKKVKPVYIALNKPVGITCTTERHVAGNIVDFVNHTERVFPIG
                     RLDKDSEGLILLTNDGDIVNEVLRAEHNHEKEYVVAVDRAFEQDFVDKMAAGVRLSDA
                     TTKPCRVQRLGAKVFKITLTQGLNRQIRRMCEALGYTVEALQRVRIMHIKLGELPLGR
                     WRQLTPQEIAPLLPKEQRSQQPQQHQPQQRPKQPQQRPQQPQHRSHPHGAHSHGAHRP
                     GGGPSRGRGQRRGRP"
     gene            19508..21457
                     /locus_tag="VFV99_12665"
     CDS             19508..21457
                     /locus_tag="VFV99_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5060210.1"
                     /translation="MRYLVLPAVRLLLITPPMTQLNTPYPATAYLMGFLRQHAAELDL
                     ELAQADASIELFLRVFSRAGIQRILDELTARAADTDEEMPPSIAHFLEHGAAYVATVD
                     SVVRFLQGSDPGFALRVVGRELLPEGPRFASTDQGPGESDDDPLSWAFGGLGIADRAK
                     YLASLYIDDLADVIRDGIAPDFELARYAERLAASAPTFDALGEALEGEPTLVDTMLDE
                     LTAELHARHRPDVVGLSVPFPGNVYGALRIARRMKALAPATRVILGGGYVNTELRELS
                     DPRVFEYVDFITLDDGESPMRALLDHLRDPAKPLFRTFVRDGGAVRLVSDGEHHDVPI
                     AAAGTPTYTGLPLTRYLSLFEMLNPMHRLWSDGRWNKLTIAHGCYWKQCTFCDVSLDY
                     IARYDRAPADLLVDRIEAMIAETGQTGFHFVDEAAPPAAMEALAKRLIERNVIITWWT
                     NVRFEKAFTPELAELLARSGCVAVSGGLEVASDRLLKLMKKGVTVEQVARVTRAFTDA
                     GIMVHAYLMYGFPTETAQDTIDALERVRQLFAEGCLQSAFWHRFTATVHSPIGKQPEL
                     FGIKLRKGAPMPTFAKNDVAFDDPTGCDHDFFADGLRKAVYNYMHGLGLDTDPRTWFR
                     RGRGVAVPKVSVAPDLIRRALAANE"
     gene            complement(21408..21749)
                     /locus_tag="VFV99_12670"
     CDS             complement(21408..21749)
                     /locus_tag="VFV99_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060211.1"
                     /translation="MIVYAIAIVIFLAAAKLHGRQLRLEREHAGVRSVDFRRRSENPP
                     FVVALWRKDRWTFWPVAAAVAISLPLVVPDVPWPLARIAFPMTTGFVVAWSFSSIRLR
                     RARAGSDQAPR"
     gene            complement(21746..22399)
                     /locus_tag="VFV99_12675"
     CDS             complement(21746..22399)
                     /locus_tag="VFV99_12675"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5060212.1"
                     /translation="MANQRGNVAVYLLFSLAVATGAGYGVYRLMRDHKPAVAANDHVD
                     VQTAPAPTPRPSHAPSDELPVEPPQAVDPAENTAVAPEDIADPPKDTVLLGTPGVAGG
                     LEPASVERMVKRYVGRYDRCMRHTHEKYRMRRGALRVTFVINATGSVDYASAQATDVE
                     EDLANCVLDVFKKLRFDKSSDGAKVKVVYPMAFVPAQGGDAGDPYPAEPPADDPLAQ"
     gene            complement(22431..23372)
                     /locus_tag="VFV99_12680"
     CDS             complement(22431..23372)
                     /locus_tag="VFV99_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5060213.1"
                     /translation="MVRVLGFALLLSSCIGSAPNGGGGSHPDGGMTSGDGTANPDAPS
                     GGLESGSLTVSWMHGSANCSQNTDPEVQVHAYNATVHIIRQNKCKTFEAPFIYVIEGT
                     TKALMIDSGATNTTTLRDTVRGLIGTKQLIVAHSHAHGDHTAGDTTFSGQANTTVVAK
                     TIAAIQAQFAIATWPTSAGTIDLGGRVLDVLAIPGHEAQHIAIYDRRTGLLFTGDTLY
                     PGLLFINDWATYRTSVSRLAQFAATHPIAHVLGAHIEMTSTAKQVYPYGTTYQPAEHV
                     LQLTAAHISELDTALTQLGPTKPTQQIAHDDFVIDPQ"
     gene            complement(23456..24082)
                     /gene="can"
                     /locus_tag="VFV99_12685"
     CDS             complement(23456..24082)
                     /gene="can"
                     /locus_tag="VFV99_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX5060214.1"
                     /translation="MLEECFEANKRWSKASLAKDPEFFARLEGLQNPDLLWIGCSDSR
                     VPANTIIGRRPGEVFVHRNVANLVEHTDVNCLSVLQFAVDVLQVKHVIVCGHYGCGGV
                     RAAMSPHPLGLIDNWLRHIRDVHLWNRDEINALQDPKARLDRLCELNVEAQVANVCHT
                     TIVQDAWRRGQNVTIHGWIYSLSDGLLRDLGMVIDSPDQIPPEYRYSY"
     gene            24183..24827
                     /locus_tag="VFV99_12690"
     CDS             24183..24827
                     /locus_tag="VFV99_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060215.1"
                     /translation="MSDFVGVGILAIAVGSAAVGAGRKLLERRRARRALREKAPLAAS
                     SPEGEVVRVTGSVRVLDQMLTAPLSGRACVVYRSRVQSGGKLTSRAMRPRESIAMVPF
                     IIDRGPEGHVLVEGKHVLLDIPPLALKRQEMERARREQFFVQHGFVLREIGHAHFEET
                     VVEPLMRVSVAGLMMKDLPTAPASEELGFRDAAPPNLRIAGNVEHPLVIGEPVD"
     gene            24845..>26403
                     /locus_tag="VFV99_12695"
     CDS             24845..>26403
                     /locus_tag="VFV99_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060216.1"
                     /translation="MSSTTWKRLLWLATCGVFVACASAPAPTPTKKKKKKKRSKDKVA
                     LKKKKEEKRDDEEQPDKPSKADKEDKADEDTVIVSDESDEETATKKKKKAKDEKKDEE
                     VAAKSKKKKKDDEENSSKKDPADEETEESAVKKKKKAKDEEDGEEVASKSKKKKDEAK
                     RKKKEAEEEEARKKKEAEEEELARKEAEEEARKQKEEEELAAKAKKKKKKKSDETKVA
                     AKENGDEEEPADEEVAEEEPKAKKKKSVKVAKAAKKNADVDMTSDENTIDMEDESSGS
                     SLDSRVEDQPPERRAVAIAVPGVDDDSGGEVAADTTPLGTNPLVINERPLVLPKGKLA
                     VHGGLRVGVLTLPNAAGMTTSTTSTGLALGVNYGASDDAEIGLDYTAGLSPGSAKGPV
                     TLHAAYNAIKGKLDMAVAGALAVDFSPATMTVPSKASFALELGAWARYRVSRKASVFS
                     GVPALPNSTVSLTKLSFPLPPLPYQLAIGLNNAGTIALDLPVGVAYQATPKIYAFGLL
                     DLAHIRIANTE"
BASE COUNT         4420 a         8979 c         8685 g         4319 t
ORIGIN      
        1 gcgatgaacg gcgatcagct gtcaccgggg cagtacgcgg tgagcacgag caaccgcaac
       61 ttcgagggcc ggcaaggccg cggcgggcgc acgttcctcg cgtcgccgca gaccgctgcc
      121 gccgctgcga tcacgggccg catcaccgac gtgcgcacgc tcctgggagg tgcccgatga
      181 acgcgttcac cacactcacc gcgcgcgcgg tcgtgctcgc cgccgacgat gtcgacaccg
      241 atccgatcat ccctgcccgc ttcctcaagg gcacgtcgaa ggtcgggctc gcgaagcatc
      301 tgttcgccga ttggcggacg cagccgggct tcccgctgct cgccgccgct gatggtaccg
      361 acgcgcagat cctcgtcacc ggcgtgaact tcggttgcgg ctcgtcgcgc gagcacgcgc
      421 cgtgggcgct gatggattac ggcttccgcg cgatcatcgc gcggtcgttc gccgacatct
      481 tcaagcagaa cgcgacgaag aacggcttgt tacccgtcga gctgccgggt gccgtgtgct
      541 tgcagatcga gcagctggtc aaccgcaatt cctcgactca gctgcgcatc gacctcgcca
      601 cccagcgggt cacgttgccc gacgggaatg cgttcccgtt cgagatcgat ccgttcgcga
      661 agcactgcct gacgaacggc atcgacgagc tcggctacat cctgtcgttc tccgagcgca
      721 tcgccgcaca cgaacaacga tgacgatcgc gatctacgac acgacgctgc gcgacggcac
      781 gcaacgcgag ggtatctcgc tcgcgtgttc cgacaagctg cgcattgccg agcaactcga
      841 tgcgctcggg gtcgccttcg tcgagggcgg ctggcccggc tccaatccga aggacgtcga
      901 gctgttccgc cgtgcgcgcg atatcgagtg gaagcacgcg acgctcaccg cgttcggtgc
      961 gacccgccgc gccggcatcg cccctgaaga cgacgcgaac ctcgccgcac tgatcgaatc
     1021 gggcgcacgc gcctgcacga tcttcggcaa gacgtcgctg ctccacgtgc gcgaggtgct
     1081 gcgcacgacg cccgacgaga acctcgcgat gatcgaggac agcgtgcgct acctcgtcac
     1141 gctcggccgc cgcgtcatct acgacgccga gcatttcttc gacggtgccg acgaggacca
     1201 cgcgtacgcg ctctcgacac tgctggcggc ggtccgtggt ggcgcggaag ccgtcgtgct
     1261 gtgcgatacg aacggcggca cgctgccgtg gcagatcacg cagcgcgtga gcgcggttgt
     1321 cgccgcgctg cccgtcgccg tcggcatcca cgcccacgac gatagtggct gcggcgtcgc
     1381 caactcgctt gccgccgtgc gcgctggcgc gacgcaggtg cagggcacgc tcaacggtta
     1441 cggcgagcgc tgcggcaacg cgaacctcac gacgatcatc ccggcactcg agctcaagct
     1501 cggctaccgc tgcttgcgtc ccggtgcgct cgccgagctc ggccgtgtcg cgcggttcgc
     1561 cgccgagacc gccaacctcg cgttcgacga gcacgcgccg tacatgggcc gcagcgcgtt
     1621 cgcgcacaag ggcggtgtcc acgtcgctgc gatccggcgc tcgccgcgct cgtacgagca
     1681 tgtcgatccg acgctcgtcg gcaaccgcac ccggttcgtc gtcagcgagc tgtcgggccg
     1741 cgccaacgtg cgcgcgatcg ccgaagccca cgaagtcgac gtcacgagcg acgccgaggc
     1801 gaacgtgctc gagaccgtca aggagcgcga ggcgcgcggc tttgcgttcg agtcggccga
     1861 agcctcggtc gcgctgatga tgcggcgcgc acagcccgac tacacagcgc cgttcgagct
     1921 gctcgactac aagatcctcg ccggcaagca ggagccgagc gccgacgcag cggtcaaagt
     1981 ccgcgtacgc ggcgagctcg tccacactgc cgccgacggc aacggcccgg tcagcgcgct
     2041 cgacgcggcg ctgcgcaagg cactgatcgc cgcgttcccc gaggtcgagc acattcatct
     2101 cgaggactac aaagttcgca tcatcgatgg tcgcgctggt acggcggcca ccacacgcgt
     2161 gttgatcgac catggcaacg ggcacgatcg gtggaccacc gtcggcgcgt cgcccagcat
     2221 cctcgaagcg tcgcggttgg cgctcgtcga tggcatcgag cacggaatct ggctcgcacg
     2281 aggaaaggaa gagagcgatg aaggccacaa tcgtcttgct gccgggtgat ggcatcggcc
     2341 ccgaggtcgt ccacgaggcg cgcgccgtac tcgaggtcat cgccgcgcgc tacggccatt
     2401 cgttctcgtt tgccgagcac ctgatcggcg gcgccgcgat cgacgcgacc tgcgaagccc
     2461 ttcccggtgc gacgctcgcc gcctgcgagc gtgccgacgc cgtgctcctc ggcgccgtcg
     2521 gcggtccgaa gtgggacaac cccaaagcga aggtgcgtcc cgaacaaggc ctgctcgcaa
     2581 ttcgcaaagc gctcggcctc tacgcgaacc tgcgtccggt cggtgtgcac cctgccctcg
     2641 ccgcggtgtc gccgctcaag tccgaccgca tcgaaggcgt cgacatcctg ttcatccgcg
     2701 agctgaccgg cggcctctat ttcggctggc cgcgcttccg cgaggaaatc ggcgaccgcc
     2761 gccgcgccgt cgacacgctc gaatacaccg aggacgagat ccgccgcgtc gtgcgcctcg
     2821 cattccggct cgcgcgcacg cgcaagaagc gcgtcacctc cgtcgacaag gcgaacgtgc
     2881 tcgagtcgtc tcgcctctgg cgcgaagtcg cggtcgaggt ctctgccgag ttccccgacg
     2941 tcgcgctcga gcaccagctc gtcgattcat gcgcgatgcg gctcgtcacg aacgcgcgct
     3001 cgttcgacgt gatcgtcacc gaaaacatgt tcggcgacat cctcaccgac gaagcggcgg
     3061 tgctcgccgg ctcgctcggc ctgctcccca gtgcgtcgct cggtgccggc acgcgtgggc
     3121 tctacgagcc gattcacggt tcggcgcccg acatcgccgg caagggcatc gcgaatccga
     3181 tcggcacgat cctctcggcc gcgatgctgc tgcgtcactc gctcggtctc gccgaggaag
     3241 ccgacgccgt cgagcgcgca gtgcgcgaag cgatcgccga tggagcgcgc tgagccgaca
     3301 tgcatgcggg ccccgcgacg gcgacgctat cgaccaccga gatgggtgac gcgatccgcg
     3361 ccgggctcgg tgaagcatcc gcagcaacac gcgtcgcgca gtagagcagt tttttttcgc
     3421 gcgaattgtt gagccgcgac gccagctttt gatgcggcgc gcgatccgtt tcgtttgaga
     3481 aacgttcacg aaatctcagt tcgtcatggt ggatcacatg acgagctcga tcggtgcgcg
     3541 cagcgaaacg tggatgcgac gcaccacgtt gctcgcgtgt tttctccact tcgacttctc
     3601 gttcatgttg tgggtcctca tcggcgcgct ggggatcccg ctcgccgagg ccgcgcatct
     3661 gtcgccagcg gagaaagggc tcgtcgtcgc ggtcccgatc ctcgccggct cgctcatgcg
     3721 catcgtcgtc gggcttctca ccgatcgatt cggcggcaag cgcgtcggcg tgatggtgct
     3781 cgcgttcttg ttcctaccgc tcgcgctcgc ctggcagctc gaaccgtcgc tgcctgcgct
     3841 cgtcgtgatc ggcgcgatgc tcggcgtcgc aggcgcgtcg ttcgctgtcg cgctgccact
     3901 cgcgagccgg tggtttccgc ccgagcgcca gggcctcgcg atgggcatcg cggccgccgg
     3961 caacagcggc accgtcgtca cgaacctggt tgctccgcgt ctcgtgctcg cgatcgggct
     4021 tggcgcgacg ttcggcgtcg cgatgatcgc gctcgggttt gttctcgtcg catttagctt
     4081 gctcgcgtac gaaccggccc gccgcgctgt gcggccgcgg ttcccgtcgt tccgcgatgc
     4141 cgatttgcgc tggttgtgcc tgttctacgc gatcacgttc ggcggctacg tcgggctgtc
     4201 gagcttcctg ccgttgttca tgcgcgacgc gtatggcgtg acgccgatcc acgccggcta
     4261 catcacggcc gcgctcgcgt gctgcggcag cttcgcgcga ccgatcggcg gcatcatcgc
     4321 cgaccgcatc ggcggtacgc gcatgctcgc gatcctgctg ttcccgatcg cggctgccta
     4381 cggcgttgcg gcgagcttgc cgtcgttgcc gatgatgatc ggcgtcttcg cggtcgcgat
     4441 gatctgtctc ggcctcggta acggcgccgt gttccagatc gtgccgcagc gctttgtccg
     4501 cgacctcggc gcggtgactg gattgatcgg cgcgatcggc ggcgtcggcg gtttcttctt
     4561 gccgacgctg ctcggcagcg tgcgcggcgc gaccggctcg tttgcggttg gcttcttggt
     4621 gctcgcgaca atcgctggtc tcgccgcgat ctcgctgcgc gtactcgtct cgtcacgtgg
     4681 ccgctgggca cacgcgatgc aacccgtggt cgcaactgaa ggagtcgcac catgagagct
     4741 ctcgtcacca cactcgttct gctagctgcc acgaacgccg acgctgacgt cgtcgcaaag
     4801 cccgcgccgt tctcgctcgg ctggagtctg cgccccgccg gcccggtcaa cgtcgtccgc
     4861 tcggataccg cgatcggtct cttcgacgac ggcggcgcat cgggcagcac ggtcgcatcg
     4921 acgttccttg tcgcctacaa ggtgtcaccc gagctatcgc cgctgttgcg ctttgccgtg
     4981 atccacaacg atccggccac tggtggcact gcaaccgcca tgtcaaaccc gctggtcggt
     5041 gcaatgtggg caccgccgat cgtcgaggcg ccgtacaagc tcgcgttctt tggcgggctc
     5101 gcactgccgc tcggcgaagg tggcggcaac gccgcaaatc cgacgatgac gagcacggtg
     5161 cgttcagcaa tcgctgcgcg ctcgagcatg gataacgcaa tgtttgcgat caacgacctg
     5221 acgccgatcg tcgggatcga tgccgcctat gtcgcgaacg gcctgacgct ccagctcgaa
     5281 gcgacggtct tcgagctgtt ccgcgtgcgg ggcgaagacg tgcagcccga cgcgtacaag
     5341 acgaacttca cgtctggcgc gcaggcagcc tacttcgtgt tcgactggct ctcggcggga
     5401 gccgagctgc gttaccagcg ctggctctcg acgccggccg cggtcgagat ggcgcccgca
     5461 acccgcgaca cggtgtcggc ggcagtcggc gtgcgcggac acttcgcagt cggcggtccg
     5521 cggtggctgc ggccgggcgt gtcgtacgcg cgtggcctcg acgatccgat gaccggccgc
     5581 aactaccaga tcgtgcagat cgacgttccc tttgcgttct gaggagtcca gatgaagtca
     5641 ggcaaggcgt atctgatcgg tgctggccca ggtgatccca aactactcac gctccgcgcc
     5701 gccgaggtgc tgcgccaggc cgatgtcgtg ctcgtcgacg acctcgtgca tcccgacgtg
     5761 ctctctcacg cacgcaccgc gcgcatcatc cgtgtcggca agcgctcgca acggcgtgcg
     5821 ttcccgcaag agctcgcgac gaacatcctg atcgcgtacg tgcgcggcgg cgcgatcgtc
     5881 gcccgcgtga agggcggcga tccgatgctg ttcggccgcg gcggcgagga agcgctcgcg
     5941 ctcgcagaag cggggttgcc gttcgagatt gttcccggcg tgacagccgc actcggagcg
     6001 gccgcctatg cggggattcc gctcacgcac cgcggtaccg cgtcgagtgt cgcgttcgtc
     6061 gctggtcacc gcgaaggaga tcagccggcg cggctcgatg tcgaggcgga cacgattgtc
     6121 gtctacatgt gccagcgcac gattcacgcg atcgcgcgcg agctgatcgc cgctggccgg
     6181 tcgccgatga catgtatcgc gctcgtacgt ggcgcgagct ggcagtcgca ggagctctac
     6241 accggctacc tcggcgaagc ggccgctctc gaggacgact ggtacgccgt gctcgatccc
     6301 gaaccgccga cggtcgccat catcggcgcc gttgccagct atgcggatca gatgcagtgg
     6361 ttcggccggc gtgcgacgcc gctcgcgatg ttgatggctt cgccgctgcg ttacgcggcg
     6421 ggaggtcgct gatgagccgt ctcgtcgtca tcggcaacgg catggtcagc catcgcttct
     6481 gcgagaagct cgtcgagtac gggctgatcg cgcaccacga gatcgtggtg ttcggcgagg
     6541 agccgcgccc tgcgtacgat cgcgtgcacc tcaccgagta cttcggccac cgatccgcag
     6601 atcagctccg actcggctcg caagcctggt atgcggaaaa ccacatcgcg ctgcgcgtcg
     6661 gcatccggat cgcgcgcatc gagcgcgagt gccgcgtcgt cgtcaccgag gcgggcgaac
     6721 ggatcgcgta cgacacgctc gtgctcgcca ccggctcggc gccgttcgta cccgcgatcc
     6781 ccggcgtcga taaggcaggc gcgttcgtct accgcacgat cgacgatctc gacgcgatcc
     6841 aaggctgggc gtcgcgcgca caacgcgccg ccgtgatcgg cggtggtctg ctcggcctcg
     6901 aagcagcccg cgctctcgtc gaagtcggca tcgacacgca cgtgctcgag gtcgcgccgc
     6961 gcttgatgcc gaggcagctc gacaatgctg gcgccgcgct cctcgcccgc agcattcagc
     7021 ggctcggcgt cacgctgcac ctcggcacgc aggtcaccga gatcggcggt gatgatgccg
     7081 tgcgctgggt ccagctcccc gatcggcgac tccatctcga catggtgatc atctccgcgg
     7141 gcatccggcc gcgtgacgag ctcgctcgcg aggcaggcat cgtgtgcggc gaccgcggcg
     7201 gcgtgatcgt cgacaagcac atgtgcacca gcgatcgcca cgtcttcgcg atcggcgagg
     7261 tcgcactcca tcgcggcacg gcctacgggc tcgtcggccc gggctacgag atggccgacg
     7321 tgctcgcgaa acgcatcgcc ggccacgacg ctgccttcac cggcggcgac cagtcggcaa
     7381 agctcaagct gctcggcacc gatgtcgcga gcttcggcaa tccgttcgcc gacgatcctg
     7441 gaacccgtgc gatcgcgttc gaggatcagc tgcgcggtgt ctatcagaag ctgatcgtca
     7501 cccgcgacgg catgcgcctc gtcggcggca tcctcgtcgg cgatacgtcc gagtacggcc
     7561 gcttgctgca cctcacccgc gcaaagcagg cgttgaacgg cgaggtgccg ctgttctcgc
     7621 gcggcacggc accgagcggc gagctgcccg acgatgtcca ggtctgctcg tgcaacaacg
     7681 tcagcgcgtg cacgatccgc gaacgcgcgc gcgccggcga cacgatcggc gagatcaaag
     7741 catgcacgaa agcgggcacc ggctgcggcg gttgcctgcc gctcgtcgcc gagctagtgc
     7801 aagtcgagct tgcccgcagc ggccgcgcga cgaagcgcgt gctgtgccag cacttcgcgc
     7861 acacgcgcca ggagctattc gagatcgtcg ccgcgacgca gatccgcacg ttcgaggagc
     7921 tgttgcgcgc gcacggcacc ggtactggtt gcgagatctg caagccgacc gtcgcatcga
     7981 tcctcgcaag catccacaac gagccgatcc tcgacgagcc acacaggccg ctccaggatt
     8041 cgaacgatcg ctacctggcg aacatccaga agaacggcac gtactcgatc gtgccgcgca
     8101 tgcccgccgg cgaggtcacg cccgagcagc tgatcgcgct cggcaacgtc gcgatcaagt
     8161 acgggctgta cacgaagatc accggaggcc aacgcgtcga cctgttcggt gcgcgcgtcg
     8221 accagctgcc tgggatctgg gaagagctga tcgcggcggg cttcgaaagc ggtcacgcgt
     8281 acggcaaggc gatgcgcacg gtgaagagct gcgtcggctc gacgtggtgt cgctacggcg
     8341 tgcaggactc gacagcgttc gcgattcgca tcgaacatcg ctacaagggc atccgcgcgc
     8401 cacacaagct gaagagcgcc gtgagcggct gcgtccgcga atgcgcggag gcgcagggca
     8461 aggacttcgg cctgatcgcg accgaacgtg gctggaacgt ctacgtgtgc ggcaacggcg
     8521 gtgcaaagcc acgccacgca gacctgctcg cgagcgacat cgacgaggac accgcgattc
     8581 gttacctcga tcgcttcctc atgttctaca tccgcacagc agatcggctg acccgcacgt
     8641 cggtgtggct cgacaagctg cccggcggca tcgagcacct gcgcgatgtc gtcgtccacg
     8701 attcgctcgg catcgccggc gagctcgagc gccagctgca gcacctcgtc gacacctacg
     8761 agtgcgagtg ggccggcgtc gtacgtgatt cggcgaagcg cgcgcagttc catgagacca
     8821 gggcgacggt gaggctgccg gttgtcgatc atccgctcgg gcgccagttc gtgcgcgtcg
     8881 cgtcgctgcg cgatgtcccc gcggaaggcg ggatcgcgat ccagtacggc cacgcgcagg
     8941 ttgcggtgtt ccgcgccggc ggcgagctgt acgcgacgca gaacagttgc ccgcacacgc
     9001 aggcgtcagt gctcgcgcgc ggcatcgtcg gagacgactg tggtgcgccg aaggtcgcgt
     9061 gcccgctgca caagcgcacg ttcgatctgc gctctggcgc ctgcctctcg caggatgcgc
     9121 caccgatccg gacattcccg atccgcgtcg acggcgacga catcctcgtc gagctgccac
     9181 ctcccgatgc gctggcggcg tgtgctggcg agaaggccct cgcgagctga tgttgcgcct
     9241 gcctctgctc gatcagtacc tccgcgagca gcacgacctc accgcggtcg aacgattctc
     9301 gcgagcccac gacacggcac tcgatggcga ccgctaccgc gagctgatgc ccgcgacacc
     9361 tcccggtgac ggtcagcagt acgcattcga ggtcgatctc gacgcctgct ccggctgcaa
     9421 ggcgtgcgtc accgcatgcc ataacctgaa cggcctcgac gagggcgaga cctggcgcgc
     9481 ggtcggactt cttcatggcg gcaccgccgc ggcgccggtc aagcaagtcg tgacgtcggc
     9541 gtgtcatcac tgcctcgatc cggcgtgcat gaacggctgc ccggtgcgcg cgtacgaaaa
     9601 ggatccggtc accggcatcg tcaaacacct cgatgatcag tgcatcggct gccagtactg
     9661 cacgttcacc tgcccgtacg aggtcccgca gtacaacgcg aaacgcggca tcgtgcgcaa
     9721 gtgcgacatg tgcagcgatc gcctcgctgc ggatgaagca ccggcgtgcg tgcagtcatg
     9781 cccgaacgaa gcgatcgcga tccgcgttat cgatcagcgc caggcgctcg acgacgcaca
     9841 aggcgacgcg tttctgcccg gcacaccgtc gcccgcgatc acggtaccga cgacgatcta
     9901 ccggaccgca cagccaccgc cgcggaactt gttacccgcc gatttctaca gcgtcgcgcc
     9961 gtcgcatcac cacgtgccgc tcgtcgtgat gctcgtgctc acgcagctgt cagtcggcgc
    10021 gttcgtcatc gacgccgtag tcactcgctt catcgcgccc cacgtgttgc aaccgatcca
    10081 cgcgctcgcc gcgctggtgg tgggcctcgc cgccctcgcc gccagcgtgt tccatctcgg
    10141 ccggccgctc tacgcgttcc gcgcggtact tggcctcggc acgtcgtgga tgagccgcga
    10201 aatcgtcgcg ttcggcgcgt ttgcaatgct ggcaatgagc tacgccgcct tgtccctgac
    10261 cggtgctgca ccggtcgccc tgcaaacggc acttggcggt gccgtggccg cgatcggggt
    10321 catcggcatc ttgtgctcgg cgctcatcta tcacgtcacg cgcaaagcct ggtggaccgt
    10381 atcgatcacg ggcttcaagt tcttcatgac cgccgcactg ctcgggctcg ccagcacgat
    10441 cgtgacgctg tcctcatcgc acacgggcgg cgatctcgcg ccaaggctgt cgcgcctcgt
    10501 cgtcctcgtc agcatcgtca agcttctcgg cgagctcgtc gtgctcgtgc acctgcgcga
    10561 caagcgctac acggagctca agcgcagcgc cgtgctgctc gtccgcgatc tgcgccggtg
    10621 gtcgatcccg cgcgtcgccg cgctgatcgc cggcggcatc ttgctgccac tcgccggcga
    10681 gccaacgctc gtacccgcga tcgcgagctt cgtcctgctc gtgatcggcg agctgctcga
    10741 acgcacgctg ttcttcgcgg cggcgagcgc gcccggcatg ccgaagggag tgcgatgaag
    10801 tcgctgcgtc atctgctcta tcaacgcgac ggcgcgctca cacacgatct tgcccgcgtc
    10861 cccgcgggct tcggcatcgg tcaggtacct gtgcggctcc agcccgattc ggtcacgagc
    10921 atgatctgcg gcttttgctc gacgggctgc tcgctcgaca tccacatccg cgacggccgc
    10981 gcagtcaaca tcacgccgac cgccgactac tcggtcaaca caggcgcagc gtgcccgaag
    11041 ggctgggatg cgctctcgcc actcggcgcc accgatcgcg cgacgacccc gctgctccgc
    11101 tcggccgccg gcaacctcga gcccgtgtcc tggccaaccg cgatcgacgc gttcgtcacc
    11161 cgcatgacag cgatcaaggc gaagtacggc ggcgaggcgc tcgcattcct cggcaccggc
    11221 cagatgccga gcgaggagct cgcgctgctc ggcgcactcg cgaagttcgg catcggcatg
    11281 gtgcacggcg acggcaatac ccggcagtgc atggcgaccg cggtcgtcgc gtacaagcaa
    11341 gcgttcggct tcgacgcgcc gccgtacacg tatgccgatt tcgaagaatc ggacgtgctg
    11401 gtgttcgtcg gtgcgaaccc gtgcgtcgcg cacccgatca tgtgggctcg cgtctgcaag
    11461 aacccgcacg atccgacgat catcgtcgtc gatccgcggc gcaccgagac ggcgatggcc
    11521 gcgacgcttc atttgccgct gcgaccgaag tcggatctcg tgctgctcta cgcgatcgcg
    11581 aacgtgctcg tgcagcgcga ctggatcgat cgcgcattcg tcgacgcaca cacgaacggc
    11641 tttgccgagc tcgcgacaca tgtcgctccg tacacgctcg atcgcgcgag cgaggccacc
    11701 gggctgccgc cggaagcgat cgagcaagtc gccgcgcaca tccactgcgg caagcgtgtt
    11761 tcgttttggt ggacgatggg cgtcaaccag agtcacgagg gcgtgcgcgt cgcgcaagca
    11821 ctgatcaacc tcgcgctgct caccggcaac atcggtcgcc cgggcaccgg cgcgaattcg
    11881 atcactggcc aatgcaacgc gatgggctca cgcctgttcg cgaacacgac gaacctcctc
    11941 ggcggtcacg acttcacacg cgccgaccac cgcgacaagg tcgccggcat tctcggcatc
    12001 gacagcgccg cgatctcgac gaagaacagc ctcgcctacg atcagattct cgaagcgatc
    12061 aaccgcggca agatccgcgc gctgtggatc gtcgcgacga actcggcgca ttcgtggatc
    12121 aaccagagcg atgccgtcga cgtgctacgc cgactcgacc tgctcgtcgt ccaggacatg
    12181 tacgcgacga ccgagactgc acgctgcgcg catctcgtgc tgccggccgc gggctggggc
    12241 gagaaggagg ggacattcat caattcggaa cggcgcctcg gcctgatcaa gcgcgtcgcg
    12301 catgcccccg gccacgcgct cgccgacttc tacatcttca agctgatcgc tgaagcctgg
    12361 ggttgtggcg atatgttcga gcgctggtcg tcgcccgaag cggtgttcag actgctcgcc
    12421 gaagtcacgc gcggtcagcc ctgcgacatc accggcatca ccggttacga cgacatcgag
    12481 cgtctgcgcg gcgtgcaatg gccggcgcct gctggcgaat cgatcgcgcc gggcagcgag
    12541 cgccggttgt tcgctgacgg ccggttctgt catcccgacg gcaaggcgcg gttcgtcgtc
    12601 ggcgagccac aagcgccgcc cgagcagccc gacgcgacgt atccattcac gctgctcacc
    12661 ggtcgcggca gctcgagtca gtggcacacg ctcacgcgca ccggcaagtc acccgtgctc
    12721 gagaagctcg gcacgagcac gccgtacgtc gagctcagtc ccgtcgacgc agcggcgcta
    12781 cgcatctcac cgggccagct cgtcgtgatc gagtcgcgac gcggtcgcat gcctgcgcgc
    12841 gcattcgtca cgcacggcgt gcagccgaag cacgtgttcg tgccgatgca ctacgcacgc
    12901 acgaaccagc tcacgtttcc cgcgttcgat ccggagtcgc gccagcccgc atacaagtcg
    12961 tgcgcggtcg cggtgcgcgc aattcgcgaa ggcgagcgcg tcgaagatct gtaggtagat
    13021 tcgttcgtga gctgcattgc gctcgctgcg ccgtgcgttg cgttgtcagg agcaaacgca
    13081 ctcgggcagc atcccgcggc gtacactcgg taccgtgtcg ggactcggtc gcgagctctt
    13141 gagatcgctg gccggcaccg gaatcgtgcc gcggctggtg gccgagcacc cgcgctccga
    13201 gtggagattg ggtgtgatga cggtcgcggt caccgcgcgt ggcgcggagc tccactatgc
    13261 acgcgagcga gtgggcgtag cgaaggcgga ggccgctgac attgtcgatg ccatgcgccg
    13321 cgcgcgcgag cgactggagg agcgctcgct cgctcccgac gtactgttgc caaagctcgc
    13381 ggcggcctac gcagcggtcc tcgcgcgacg aggcgcacgg cccggcgaac gggtgccgct
    13441 cgtcgacgtc cgtggcgagc tgtcgacgac gcgcgcgcag ttcgcgtggg acgtcgcccg
    13501 gctcgcgcgc gacaaacgcc tggtcgtcga tggctgccgt attgatttcg ggattgcgac
    13561 cgggccgcga attgctcgtt cgcaaaccgt gtggctcgag aacgacgccg gtggtggcgc
    13621 gttcttcgcg acgttgcgac tgataccgga ggctccgcat gatcagacca ccgaaactgc
    13681 cgaccgaccc cgatcgcaag ctcgcgcgcc ggatcctggc gcagatgggc gagaacggaa
    13741 aaccgcccga gctcggaatc tcacacgtga acgtgggaaa cgagtcgtac ctgcaggtga
    13801 tcgacgccgc gtacgtcggt gatctgatcg gcgtcgtcga ggggtcgagc ttcaagatcg
    13861 tgcagggcac gtacggtgcc ggcaagacgc acttcctcta ttgcgtccgc gacctggcgt
    13921 ggcgacgcgg tttgctcgcg gcgttcgtga ccatctcgcc gaaggagtgt ccgttcaacc
    13981 gaccgctggc gatctacaag gccatcgcga atagcatcga gctaccgcga ggcacgggcg
    14041 gcgagatcac gcgtggcgtc gacgacgtga tccgcgcgaa cctcgaggcc cggatcgctg
    14101 aggagggtcg cgatgcgacg cgcgcctggc tcgacaagca actcgcacgg gcacagatca
    14161 cacgccactc gttccgtgac gccgtggttg gattctcgcg cgccgtgctc gacaacgacg
    14221 agcccaccgc tcgcttgctc ggggcctggc tacgcggcga ggacgtttcg gtctccgagg
    14281 tcaaggcggc tggagtcttc gagctcccgt ccaacgaaaa tggattcggc atggtgcggt
    14341 cgctcgtcca gctggttcat cagctcggct acgaagggac cgtcgtgctg ctcgacgagg
    14401 ccgagcgccg gctctcggtc gatggcaagc cgaccaagac gatggcagag accatggatc
    14461 acctgcgcga gctggtcgat ctgtgcggac gctcggagct accgcgcacg ctcgtgctgt
    14521 atgcggtgac gccggcgttc acggccgacg tgcttccgca atatccagcg ctgcagcagc
    14581 ggctcggcgc gccgatccag tttctcagca cgacgaatcc gaaggctccg gtgatcgatc
    14641 tcgaggcgct cgatctcgag ccacgcaagc tgcttgtcga gctcgggcgg cgcctcgtcg
    14701 tcgtcgccaa gcgcgcctac gagtggtcgc ccaacgacaa gctcgtggag cgcaacctcg
    14761 acctgcttgc atcgatcgtt gccgaagaac agctcgaggt cggtcaccga cgcttcttcg
    14821 tgaagctctg catccggcta ctcgatcagc aacggatcgg cggcgagaag aagctcgacg
    14881 acgaccatat ccaggcactc gtgcgcgacg aacaggcgct ccagctcgag gaagcgcgcc
    14941 cggacgagta cggcgagttc ctcgggatca catttcgcaa gaagaaggac tgaggcgtca
    15001 gttgcagtcg ctgcagctcg ggctctcacc tggttcgcag acaccgttcc cgcagatcgg
    15061 cgcacacgcg ctcccgatca gctcgcaaca atcgctcgca cacgctgcgt gggtctcgcc
    15121 tgcttcgcag taaccgttgc cacagaccgg aacgcagtta ctacccgaac ccgtgtcgca
    15181 gcagtcactc gcacacgacg catagctctc gccgaattcg cagtagccgt taccgcacat
    15241 cgcgctgccc gatccggagc cactgcccga gccagatccg ctgcccgagc cagatccact
    15301 gcccgagccc gagccgctgc ccgagccaga tccactgccc caaccgctgc ccgagccaga
    15361 tccactgccc cagccgctgc ccgagcccga gccgctgccc cagcccgagc cactgccact
    15421 gcccgagccg gacccactgc ccgaaccgct tcccgagcca tgccccgagc cactgcccga
    15481 gccgggatct ttcggcggtg gacgcggcgg cggctgagtc ggctgcttga cccacgacca
    15541 acacgagaac gggtcctcgc atgaccagca gccgttgcgc accgccgcga tgcgatgacc
    15601 gctgcacgtc ggtggttctt gcgtgcagcg aagcggcgag ccgtcgttgc acgtgcgcgg
    15661 gaagccgagc ggtccgcacg tctgctcgtc ggcgcacgtc cagcagcctt gctggatcgc
    15721 gagcaccgtg ccggccgggc acgacggagc ccaccatccg cacgacatcg gcgagccgtc
    15781 gtcgcagtag cctgcaccgc cgtgcgcagc gcgatccgcc gactcgtcgg tcgcaaggtc
    15841 gtttggcaac gcattcgcga acatcacggt gttcatgaac gaatcgaggt gaagcgcggg
    15901 cgctcggcgc ggcggcacga agcgagcggc ggcgggcgca acactttcgc tgcgtgacga
    15961 cgctgcgacc atcaccggtg tcggcacatc atccaccgcc ggtgcagcaa cgactcgctc
    16021 gcgatactcg acgaccgctt gggcgaccgg ccgttcgacg atgcgcgtga tcgtaccggt
    16081 cgtacctggc agcaccgata cagcagctgt gacgagcatc gtcgcgccga cgacggcgac
    16141 tgccgcaccg cgcttgcgcg acggtcgtat agcggccgcg agcgccgcga ggagctcggc
    16201 catcgacggg tagcgatcat ccgggtgcac tgcgagcccg cgctcgatca cgcggtgcac
    16261 ggcgcggagc gccgctgcgc catcgagtgc ttcgcgcagc gcgacgcaga acgagtattg
    16321 atccgagcgc gcgtccgtca ccgcgcccgc gcgttgttcc ggtgccatgt acgccggcgt
    16381 accgacgatc gcacgcgacg tgcttgcacc gatgatgcgc gcgagcccga agtcggtgac
    16441 gcgtacgcgg ccgtcgtgat cgacgagcac gttgtcgggc ttgaaatcga ggtgcacgag
    16501 ccccgcgcga tgtacgtatg cgaggccttc gcccgcggcg agaaacagcg tgacgatcgc
    16561 gcgccaatcg cgcggctgtt cggtgaccca cgcgcgtagc gtcgagccag cgacgagctc
    16621 catgcacacg tagagctgac cgttcgcgat gccggcgtcg tggacagccg cgatgttcgg
    16681 atgctgcagg cgcgccatcg cttgcgcttc ggcgagcaag cttccgcctt cgagcaagtc
    16741 gggccgcaag agcttcagag cgacgcggcg agcgagcgtg gtgtcctgcg cttcgtagac
    16801 aacgcccatg ccgccgacgc cgatgacgcg cacgatctcg tagcgcgcga tcgattcgcc
    16861 cggacgcggc aggccatgcg cgagcgagcc gggtcgcgcg ctaccgatcg ccgacagtcc
    16921 gcggccgaga ttcgcgacga gccggcggca cgccgggcac tcgtcgaggt gcacttcgag
    16981 gcgctctgcc gcagctgggt ccgccgcgcc ggcgagcagc gccgcgatcg tgttggttcg
    17041 tgcacaactc tgcatcggag cgggcatgcg cgattcagat ttcgcgccaa gcagcggacg
    17101 tgacgttctc agcggtttgc gccggtcagc catcagtttt tcgaggctgc ggccgcagga
    17161 tctcccgcag agtgacatcg gccatgctga cgagcattcg aaagatgctg tctagctctg
    17221 tctcgccgat gcccagtgca agccgcatct gttcccgggt ccgcgcgatc agggtctcgc
    17281 gggtctgctt gagctgacgc gcagcggttg cacgatgcac gtgatagatc gcggcgatct
    17341 ggtcgatgct gagcccgtcg atgacggagt ggcgcaacag gttgcgctcg cgcggtgtca
    17401 gcgtcgtgac ggcctcgcga aacgcggccc gaaattgcgc gccgtagtgt tgctgcagat
    17461 acgcgagctg cggatcggga acacccggtg cgagctgatc gaggtgctcg tcatcgagcg
    17521 gttggttgcg gccgtcgtgc gccgcttgtc gcatgccgag tcgcgccgca cacacgcgca
    17581 accagatcgc gagtggcgcg cggccgtcgt atgcggcgat gccgggtgca ccatcgcgcg
    17641 cgaccagcat ctgctcgcgc agcatctgga tgagctcgcg gcgcgcatcg tcgctctgcg
    17701 cgatcttggt cagcccgcgc atcacgatcg gcagcgtctc gtcctcgagc gtcgcgagcg
    17761 cggctcgatc accacgcgca caaccgcacg cgagatagag atcctcgacg tgacgacgct
    17821 cgagctcggc gcccggctcg ctcgtcgcga tgcgctcgcc ggcgaacgtc atcaacgcgg
    17881 tctcgtcgac ggcgagttgc ggatgtgccg ctcgcgcgcg ttgccatgcc gcggtgacat
    17941 gcggaacgag cacatcgagc gatgcagggc gatgcagggc ggcgagagcc gtccagaatg
    18001 cgcgcactgc gtcgctcgac accatcgtcg acggatgtac cacggcggcg atcacggacc
    18061 gctctagtcg cccgaacccg atccggatcc accgcccgag cccgagccac tgccagagcc
    18121 ggagcccgag ccactgccag accccgagcc cgagccactg ccagagcccg agccaccgcc
    18181 gcaaccggag cccgatccgc tgccccaacc agagccggag ccgctgcccg aacccgagcc
    18241 ggagccactg ccccaaccag agccagagcc actgcccgaa ccggatcccc aacccgagcc
    18301 actgcccgaa ccagagccgg agccactgcc ccaaccagag ccggagccac tgccccaacc
    18361 agagccggaa ccactgcccc agccagagcc ggagccacaa cccgagccgt ccgagccacc
    18421 cgagccgtca tcacccgacg acagcgacgt ggcgctcggt ccgctgacgt ctggttcatc
    18481 gacacaggcg acgaagccgg cgacgagcgc agcaacaaac atgacgagga cgcgcataaa
    18541 cgatctcctg tgaagtcact ggagataggc agcgacccgc gaaaagtcgc gcacgcgcgc
    18601 gcggcgtcgc gttttcctat ggccgtccgc gacgttgccc gcgaccgcgc gaagggcccc
    18661 cgcccggccg atgcgccccg tgcgagtgcg ccccgtgcgg gtgcgatcga tgctgcggct
    18721 gttgcggtcg ttgctgcggc tgcttcggcc gttgctgcgg ctgatgctgc tgcggctgtt
    18781 gcgatcgctg ctctttcggc aacagcggcg cgatctcctg tggcgtgagc tggcgccagc
    18841 ggccgagcgg tagctcaccc agcttgatgt gcatgatgcg cacgcgctgt agcgcctcga
    18901 cggtgtagcc gagggcttcg cacatgcgac gtatctgccg gttgagcccc tgcgtcagcg
    18961 tgatcttgaa gaccttcgcg ccgagccgct gcaccctgca cggcttcgtc gtcgcgtcgg
    19021 acagccgcac gccggccgcc atcttgtcga cgaagtcttg ctcgaacgca cgatcgaccg
    19081 cgacgacgta ttccttctcg tggttgtgct cggcgcgcag cacttcgttg acgatgtcgc
    19141 cgtcgttcgt gagcaggatc aggccctcgg agtctttatc gaggcgaccg atcgggaaca
    19201 cgcgctcggt gtggttgacg aagtcgacga tgttgccggc gacgtggcgc tcggtcgtgc
    19261 acgtgatgcc gaccggcttg ttgagcgcga tgtagaccgg cttgaccttc ttgcgggcct
    19321 ggccaacgcg atcgccgtcg agccgaacct cgtcggtctc cgagacttgc gtaccgacga
    19381 cggcgggctt gccgttgacg gtgacgcgac cttcggcaat tagctcgtcg gcctcgcgcc
    19441 gcgagcatgc gccgctctcg ctcaggaact tgttgagacg cacttcgagc atcgacccta
    19501 cctcagggtg cgctaccttg tgctgcctgc cgtgcgcctg ctgctgatca cgccgccaat
    19561 gacccagctc aatacgccgt accccgcaac ggcgtacctg atgggttttc tgcgccagca
    19621 cgcggcggag ctcgatctcg agctcgcaca ggccgacgcc tcgatcgagc tgttcttgcg
    19681 cgtgttcagc cgcgccggca tccagcgcat cctcgacgag ctgaccgcgc gcgctgccga
    19741 caccgatgag gaaatgccgc catcgatcgc gcatttcctc gagcacggcg ccgcgtacgt
    19801 cgccaccgtc gattcggtcg tgcgcttctt gcagggcagc gatccggggt tcgcgcttcg
    19861 cgtcgtcggc cgcgagctgt tgcccgaggg cccgcgcttc gcgtcgaccg atcaggggcc
    19921 gggcgaaagc gacgacgatc cgctgagctg ggcgttcggc ggcctcggca tcgccgatcg
    19981 ggcgaagtac ctcgcgagcc tctacatcga cgatctcgcc gacgtgatcc gcgatggcat
    20041 cgcacctgat ttcgagctcg cgcgctacgc cgagcggctc gcggccagcg cgccaacatt
    20101 cgatgcactc ggcgaggcgc tcgagggcga gccgacgctc gtcgacacga tgctcgacga
    20161 gctgaccgcc gagctccacg cacgccatcg ccccgacgtc gtcgggctga gcgtgccgtt
    20221 tcccggcaac gtctacggtg cgctgcgcat cgcgcgacga atgaaggcgc tcgcgcccgc
    20281 gacgcgcgtg atcctcggcg gcggttacgt caacaccgag ctgcgcgagc tgtcggatcc
    20341 gcgcgtgttc gagtacgtcg acttcatcac gctcgacgac ggcgagtcgc cgatgcgcgc
    20401 gctcctcgat catctgcgcg atccggccaa gccgctgttt cgcacgttcg tgcgcgacgg
    20461 cggcgcggtg cgcttggtca gcgacggcga gcatcacgat gtaccgatcg ccgcggccgg
    20521 cacgccgacc tacaccggcc tgccgctcac tcgctacctg tcgctgttcg agatgctcaa
    20581 cccgatgcat cgcctctggt ccgacggccg gtggaacaag ctgacgatcg cgcatggctg
    20641 ctactggaag cagtgcacgt tctgcgacgt gtcgctcgac tacatcgcgc gctacgaccg
    20701 cgccccggcc gatctgctcg tcgatcgcat cgaggcgatg atcgcggaga cggggcagac
    20761 cggctttcac ttcgtcgacg aggcggcgcc accagccgcg atggaagcac tcgcgaagcg
    20821 cctgatcgag cgcaacgtga tcatcacgtg gtggacgaac gtgcggttcg agaaggcgtt
    20881 cacgcccgag ctggccgagc tactcgcgcg gtcgggctgc gtcgccgtga gcggcggcct
    20941 cgaggtcgcc tcggatcggc tgctcaagct gatgaagaaa ggcgtgaccg tcgagcaagt
    21001 cgcgcgcgtg acacgggcgt tcaccgacgc cggcatcatg gtgcacgcgt acctgatgta
    21061 cggatttccg acggaaaccg cgcaggacac gatcgatgca ctcgagcgcg tgcgccagct
    21121 gttcgccgaa ggctgcctgc agtcggcgtt ctggcaccgc ttcaccgcga ccgtgcacag
    21181 cccgatcggc aagcagcccg agctgttcgg catcaagctg cgcaagggcg ccccgatgcc
    21241 gacgttcgcg aagaacgacg tcgcgtttga tgacccgaca ggctgcgacc acgacttctt
    21301 cgccgacggc ctgcgcaagg ccgtctacaa ctacatgcac ggcctcggcc tcgataccga
    21361 tccgcgcacg tggttccggc gcggccgtgg cgtcgccgta ccgaaagtca gcgtggcgcc
    21421 tgatctgatc cggcgcgcgc tcgccgcaaa cgaatagaac tgaacgacca cgcgacgaca
    21481 aagcccgtcg tcatcgggaa tgcgatgcgc gcgagcggcc acggcacgtc gggcacgacg
    21541 agcggcaacg agatcgcgac cgccgctgcc accggccaga acgtccaccg atctttgcgc
    21601 cacaacgcga ccacgaacgg cggattctcg ctgcgccgcc tgaaatcgac gctcctgacg
    21661 ccggcatgct cgcgctcgag ccggagctgg cggccgtgca gcttcgccgc cgcgaggaag
    21721 atcacgatgg cgatcgcgta gacgatcatt gcgcgagcgg atcgtcggcg ggcggctctg
    21781 ccggatacgg atcgcccgcg tcaccgccct gtgccggcac gaacgccatc gggtacacga
    21841 ccttcacctt cgcaccgtcg ctcgacttgt cgaagcgcag cttcttgaac acgtcgagca
    21901 cgcagttcgc gagatcttcc tcgacatcgg tcgcctgcgc cgacgcgtag tcgacgctgc
    21961 cggtcgcgtt gatcacgaac gtcacgcgca gcgcgccgcg tcgcatccgg tacttctcgt
    22021 gcgtgtggcg catgcagcgg tcgtagcgac cgacgtagcg cttgaccatg cgctcgacgc
    22081 tcgccggttc gagcccaccg gcgacgccgg gcgtgccgag cagcactgtg tccttcggcg
    22141 gatcggcaat gtcctcgggc gcgaccgcgg tgttctcggc cggatcgacg gcctgcggtg
    22201 gctccacggg cagctcgtcg ctcggcgcgt gcgacggccg cggtgtcggc gctggtgcgg
    22261 tctggacgtc gacgtggtcg ttagcggcaa cggctggctt gtgatcgcgc atcagtctgt
    22321 agacgccgta acccgcgcct gtcgcaactg cgagcgagaa caacaggtac accgccacat
    22381 tgccgcgctg gttcgccacg gctttggagg atacgccagt cccgaaccag ctactgcggg
    22441 tcgatcacga agtcgtcatg tgcgatctgc tgcgtgggct tcgtcgggcc gagctgggtc
    22501 agcgcggtat cgagctcgct gatgtgcgcg gcggtgagct gcagcacgtg ctcggctggt
    22561 tgatacgtcg tgccataggg atagacctgc tttgccgtcg aggtcatctc gatgtgcgcg
    22621 ccgagcacgt gcgcgatcgg gtgcgtcgcc gcgaactgtg cgagccggct gacgctcgtg
    22681 cggtacgttg cccagtcgtt gatgaacaag aggcccggat acagcgtgtc gccggtgaac
    22741 agtaggccgg tgcgccgatc gtagatcgcg atgtgctgcg cctcgtggcc cggaatcgcg
    22801 agcacgtcga gcacgcgacc gcccagatcg atcgtccctg ccgaggtcgg ccacgtcgcg
    22861 atcgcgaact gtgcctggat cgcggcgatc gtcttcgcga cgacggtcgt gttcgcctgc
    22921 ccgctgaacg tcgtgtcacc cgccgtgtga tcgccgtgcg cgtgcgagtg agcgacgatc
    22981 agctgcttcg taccgatgag accgcgcacg gtgtcgcgca acgtcgtcgt gttcgtcgca
    23041 ccgctgtcga tcatcagcgc cttcgtcgtg ccttcgatga cgtagatgaa cggcgcctcg
    23101 aacgtcttgc acttgttctg gcgaatgatg tggacggtcg cgttgtaggc gtgaacctgg
    23161 acttccggat cggtgttctg cgaacagttc gccgagccgt gcatccagct gaccgtgagc
    23221 gagccgcttt cgaggccgcc gctcggagcg tcgggatttg cagtcccgtc gccagacgtc
    23281 atgccgccat cgggatggct gccgccaccg ccgttcggcg cgctaccgat gcacgaactg
    23341 agcaacaagg cgaagccgag aacgcgaacc acgaccctgc aactagcagg tatcgcgccg
    23401 gtcccggcgc cagcgatttc gccaggttca ctaccgcgac gcggggccgc ggccctcagt
    23461 acgagtagcg atactccggc ggaatctggt cgggcgaatc gatcaccatg ccgaggtcgc
    23521 ggagcagacc gtccgacagc gagtagatcc agccgtggat cgtcacgttc tgaccgcgcc
    23581 gccacgcatc ctggacgatc gtcgtgtggc acacgttcgc gacctgcgcc tcgacgttga
    23641 gctcgcacaa acgatcgagc cgcgccttcg gatcctggag cgcgttgatc tcgtcgcggt
    23701 tccacagatg gacgtcgcgg atgtgccgca gccagttgtc aatgaggccg agcggatgtg
    23761 gcgacatcgc ggcgcgcaca ccaccgcagc cgtagtggcc gcacacgatc acgtgcttga
    23821 cctgaagcac gtcgacggcg aactgcagca ccgagaggca attcacgtcg gtgtgctcga
    23881 cgaggttggc gacgttgcga tggacgaaca cctcgcccgg ccggcgaccg atgatcgtgt
    23941 tcgcgggcac acggctgtcc gagcaaccga tccacaacag gtcgggattc tgcagtccct
    24001 cgagccgtgc gaagaattcg gggtctttcg cgaggctcgc cttcgaccag cgcttattcg
    24061 cctcgaagca ctcctcgagc agcgtcttct tcggggtgga cacgaggctt gggttttgtc
    24121 atggaccgat agggttcgtc acctgctgtt gcgcagtagg atggttcaca aggaaatcag
    24181 gcgtgtctga cttcgttggc gttggaattc tcgcgattgc cgtgggttca gccgcagtcg
    24241 gcgctggccg caagctgctg gaacgccggc gcgcacgccg cgcgctgcgg gaaaaagcgc
    24301 cgctcgccgc gagctcaccc gagggcgagg tcgtgcgcgt caccggcagc gtgcgggtgc
    24361 tcgatcagat gctgaccgca ccgctctcgg gccgcgcctg cgtcgtctat cgctcgcgcg
    24421 tgcagtccgg cggcaagctc acgagtcgcg cgatgcggcc gcgcgagtcc atcgcgatgg
    24481 tgccgttcat catcgaccgc ggccccgaag gtcacgtcct cgtcgagggc aaacacgtgc
    24541 tgctcgacat cccgccgctt gcgttgaagc gccaggagat ggagcgagct cggcgcgaac
    24601 agttcttcgt ccagcatggg ttcgtactcc gcgagatcgg ccacgcgcac tttgaagaga
    24661 cggtggtcga gccgctgatg cgagtctcgg tggcaggctt gatgatgaag gacctgccca
    24721 ctgcgccggc ttcagaagag ctggggtttc gcgatgcggc gccgccgaac ctgcgtattg
    24781 ccggcaacgt agagcatcca ctcgtgatcg gtgagccggt cgattaggat tgatgtcggc
    24841 caacgtgtcc tcgacaactt ggaaacggct gctgtggctc gcgacgtgcg gcgtgttcgt
    24901 cgcgtgtgcg agcgcgccgg cgcccacgcc gaccaagaag aaaaagaaga agaagcgcag
    24961 caaggacaag gtcgcgctga agaagaagaa agaagagaag cgcgacgacg aggagcagcc
    25021 cgacaaacca tcgaaagccg acaaagaaga caaggcggat gaagacacgg tgatcgtgtc
    25081 cgacgaatcc gacgaagaga cggcgacaaa gaagaagaag aaagcgaagg acgagaagaa
    25141 ggacgaagaa gtcgccgcga agtccaagaa gaagaagaag gacgacgagg agaattcgtc
    25201 gaagaaggat ccggccgacg aagagaccga ggagagcgcg gtcaagaaga agaagaaagc
    25261 gaaggacgag gaagacggcg aagaagtcgc cagcaagtcc aagaagaaaa aggacgaagc
    25321 gaagcggaag aagaaggaag ccgaagaaga ggaagcgcgg aagaagaaag aggccgaaga
    25381 ggaagagctc gcgcgtaaag aagccgagga ggaagcgcgg aagcagaaag aggaagaaga
    25441 gctcgcggcc aaggcgaaga agaagaagaa gaagaagagc gacgagacca aggtcgcggc
    25501 gaaggaaaac ggcgacgaag aagagcccgc cgacgaagaa gttgccgagg aagagccgaa
    25561 ggcgaagaag aagaagtcgg tgaaggtcgc gaaggcggcg aagaagaacg ccgacgtcga
    25621 tatgacgagc gacgagaaca ccatcgatat ggaagacgag agctcgggat cgagcctcga
    25681 ctcgcgcgtc gaggaccagc cgcccgagcg ccgcgcggtg gcgatcgcgg tgcccggcgt
    25741 cgacgacgac agcggtggcg aggtggctgc cgatacgacg ccgctcggga cgaacccact
    25801 cgtgatcaac gagcgtccgc tcgtgctgcc gaagggcaag cttgccgtgc acggcgggtt
    25861 gcgcgtcggt gtgctcacgc tgccgaacgc cgctggcatg acgacgtcga cgacctcgac
    25921 cggcctcgcg ctcggcgtga actacggcgc gagcgacgat gccgagatcg gtctcgacta
    25981 caccgctggt ctcagcccgg gttcggcgaa aggtcccgtc acgctgcacg ccgcgtacaa
    26041 cgcgatcaaa ggcaagctcg acatggcagt tgccggcgca ctcgcggtcg acttctcgcc
    26101 cgcaacaatg acggtgccgt cgaaggcgtc gttcgcactc gagctcggcg cgtgggcgcg
    26161 ctatcgggtg tcgcgcaagg cgtcggtgtt cagcggcgtg cctgcgctac cgaattcgac
    26221 ggtgagcctc acgaagctgt cgttcccgct gccgccactg ccctatcagc tcgcgatcgg
    26281 cttgaacaac gcgggcacga tcgcgctcga tctaccggtg ggtgtcgcgt accaggcgac
    26341 gccaaagatc tatgcgttcg gcctgctcga cctcgcgcac atccgcatcg cgaacaccga
    26401 gaa
//
LOCUS       DASUFX010000319        21363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1994468, whole genome shotgun sequence.
ACCESSION   DASUFX010000319 DASUFX010000000
VERSION     DASUFX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21363
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994468"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFV99_12700"
     CDS             complement(<1..240)
                     /locus_tag="VFV99_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEX5060217.1"
                     /translation="MLTFSRDPNVAEQQMNAIIFYLTAFGYIDGNFDFTEKTFVRIYI
                     RQLVTQRAKLALPDADDKTRDEVINKFTTHFHEVFE"
     gene            300..1586
                     /locus_tag="VFV99_12705"
     CDS             300..1586
                     /locus_tag="VFV99_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5060218.1"
                     /translation="MRAGVLATLVALSSPAIADPADRLEASGFLGVEDFSKENGLGNA
                     LAPEQRPQTAPTFGGRLTYFALETSGDMHLDLGVEAELSFTPAWTGYGFKEARPSYFA
                     PVFGYRANLLLRLGGGWFQPHVSGGVGALTVASDSPLMAKETDPVFVWGLGAQFAMGS
                     GWQVRLDARQALTESVTGDTTSNLEALLSVGMRFGEKPPKPEPIEHIDVVVQKPPPPP
                     EPDKDSDNDGIPDKLDGCPAQAESVNGIDDQDGCPEPDPDGDKIVGTADKCPDRAEDF
                     DKFEDEDGCPEDDNDKDGIVDARDVCPNEPETKNGITDDDGCPDAVPPAVVEALATAT
                     KAKFDANSVRISSRIKTALDKALITMMSNAKLKYVITVHPDKDGDKDAVLANRRAENV
                     KFYLVEQGVAMGALSTTVGPVSKDKNAPQLEITIAP"
     gene            1588..2127
                     /locus_tag="VFV99_12710"
     CDS             1588..2127
                     /locus_tag="VFV99_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5060219.1"
                     /translation="MIGLVAKLLEAPLAVGDTSFEARDAIVVLGAPLTAAGELSQVLE
                     ERVAAAVALYRMGGGRLVVASGGPTHGTRPEAAAIAEALHAAGIPDVLVEDRSLSTAE
                     NARLTAEVLAPLGVRTVWIVTQPFHAKRAARLFRRAGLDARSWHIADSVQYRDRGRAV
                     RWLVREYASWAALAVRGPR"
     gene            complement(2383..3831)
                     /locus_tag="VFV99_12715"
     CDS             complement(2383..3831)
                     /locus_tag="VFV99_12715"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5060220.1"
                     /translation="MKALAVLATLAGCAPLVMEAPTPAWVNDTSSYGGPNQPRPIVEH
                     YRETANKIISTARADRGAYKKLAYLTDQIGHRLAGSPELDKAIAWAAQAMKDDGLDAR
                     TEKVMVPHWVRGAEEASITAPITRPLHLLTLGGSVATPKGGITAPVVVVHDWKELDAK
                     AESVKGAIVVYNVAMPAWTPEKGSGYGKTVEYRFAGASQAAKRGAVAALVRSVTAKSL
                     STAHTGAMGYDKEQPKIPTASITVEDAELLDRLSAQGKVTLHLRLEPQLLPDAESANV
                     IGELRGREKPDEVVVIGGHIDSWDVGQGAHDDGAGIVTMMQAVRVLKQLGLTPRRTIR
                     VVLFTNEENGTRGAKTYAETHKAELAKTVLALESDSGGFAPRGFGVGHKDPEAAKRTR
                     ARLTEVAALLAPLGKLKVDDGHGGTDIEPMAAAGVPQVGLEVAGEKYFDYHHTEADTL
                     DKVDPQDLADMVAAVAVLAYVVADLPDRVDAP"
     gene            complement(3828..5468)
                     /locus_tag="VFV99_12720"
     CDS             complement(3828..5468)
                     /locus_tag="VFV99_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060221.1"
                     /translation="MSRRRLAGGLAPIHILLLVAFLEVAINRVAVPMLRPAKGTPPTW
                     HTALDYGGLFLFYFAGTLAAVLIGVRVVEGLRTSRSVRDFIANGALAVATVLAAIPLV
                     IGAPPSLVFALEVAFAVAIIAMFSTSISHHYDVGAQLGVVTLSLPLVLHTVTVLGGKY
                     LWPEGAFDGPGVEITRAGVMALCAAALVSPYVFAPRPFARAVTRPGPVVIAMAIAALG
                     AVAARTWYPIVAKAAALAVGVELNQAQADPRLAIYLLAIATLAWTLASCAIAHSQARR
                     AIGAGIAFVILGGYAFRWPNHYLLPLLGIALVTTAVRKVREEELAALPITTDTPPIAD
                     AAWSGYVASVKSGLERSLAGVHALTTRGDGGLASSVIIGDKDGLNVRTRIERIEGSVI
                     ALDVIIGREIDELRGATLTLWAIPPRTLGINPAGPPAAPIIKTNDTTFDQRFKTRGNR
                     VAFDKLLDEGLRARVIATLDGWLAYWEPEGLRYRVYPGRGAPLDHPMPLSDLALGRTA
                     SPERLIAIIELLVDIAARGVPVEPAAAPTALDHVESSS"
     gene            5427..6317
                     /locus_tag="VFV99_12725"
     CDS             5427..6317
                     /locus_tag="VFV99_12725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5060222.1"
                     /translation="MNGGKTPCKAPTRQPEKRSVVDYLNQGARKFGTCFQVRRTKSWA
                     PRSRHEKIFSVRFCPSLLVLVGLAASAATSGAAPSRPPVVVLDPGHGGSNPGAQGVAG
                     LREKQLTLTLANLVATRLREHGIAVTLTRDTDKTLTLRQRVEVADRLPADIFVSIHAN
                     ASTTRAQSGYETYVLTAKGVDIDARALRSGTTTPRPGVDPDIALVLDDLERGASQWES
                     AELAARMQRSLRDRRGPDGDRGVRQDSHHVLLGATMPAVLVEVGFIDHPIEGKQLADP
                     QIQAGIAAAIAEAIEDQLAD"
     gene            6414..6803
                     /locus_tag="VFV99_12730"
     CDS             6414..6803
                     /locus_tag="VFV99_12730"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEX5060223.1"
                     /translation="MAVCLIATASPAFADKKADDTAAASEANVSTEVKAGTGVENHEV
                     VGEATTFEQGTTVFVWSRITDGPASIKHVWKLDGKPVWTATLPVKSKKWSTQSRRVIS
                     KPGAYEVDVTSADGVSLGTIAFTVTAK"
     gene            complement(6902..7579)
                     /gene="lepB"
                     /locus_tag="VFV99_12735"
     CDS             complement(6902..7579)
                     /gene="lepB"
                     /locus_tag="VFV99_12735"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5060224.1"
                     /translation="MTDATPSDTSTDTPSDAASPADSPAPKRSRLRRWVREISVFMVL
                     AFVLLSARSSLADHYQVPTGSMLPTVHIDDRILVNKAAYGLRVPFTSSFLTEFQGPQI
                     GDTVVLRSPEDGRVLLKRVVGIPGTTIEVHLGQISIDGKLAPIEEHYGMLVEDLAGTH
                     HLVRLTNAGGPDFGPVTLPDGKYLVMGDNRGDSHDGRSFGLVSRDAFLGRAMGVYWRG
                     GPTWDPL"
     gene            7725..8963
                     /locus_tag="VFV99_12740"
     CDS             7725..8963
                     /locus_tag="VFV99_12740"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX5060225.1"
                     /translation="MSWLTVIVLGRILAESGSPPPDVDDQTILLIKRKDVMVGCSPGD
                     AGVDMDAASDAPDGDAAIGDAALDDAGVSDAGIGDAGVDDAGVDASTCTMISGDAVTL
                     VVQPHVSTSADGTRFAVLLVTPDRPIVELAGDVFSSLAQTTAPRIDIHTVEVPDPALG
                     QQCATYESGCGGGESDGCGGDFGDDGSSWTPPKIGDAGLGDGGLVQEMIGPYQFVRAQ
                     PTSAMELAGWLDQFGYAYMQKDLDAVAPYIALGYHVVAIRVSLDHPLTAPMIPISLTW
                     AGSELRVPAALGAGAVGTGTLTVYIAAEGKYQFPNATIRFARQTSAGGAGYLTRNQIT
                     LDQNLPPEQDPIAYRVTNSDFQEVTTQTEYVHVPVDVDCSQQEERGGCCNECNAKPRN
                     RLDLLTIVVAVAFVLRRRRR"
     gene            9057..9200
                     /locus_tag="VFV99_12745"
     CDS             9057..9200
                     /locus_tag="VFV99_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060226.1"
                     /translation="MLTLLIVLLVLALLGGGLGYGRFGAVGMSPAALLVIILIVLALM
                     GRL"
     gene            complement(9219..9611)
                     /locus_tag="VFV99_12750"
     CDS             complement(9219..9611)
                     /locus_tag="VFV99_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX5060227.1"
                     /translation="MATAPHHGHDDHGHGLAHVAAIKVLLATGGTLLLLTLVTVISSK
                     IDFGGASINLAIAMVIAVTKATLVVLFFMHLRYDRLFHSVVFCSAILAATLFVGFTLM
                     DTDQYQQTNIWNPTAPPMAPVGPRAING"
     gene            complement(9620..10870)
                     /locus_tag="VFV99_12755"
     CDS             complement(9620..10870)
                     /locus_tag="VFV99_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5060228.1"
                     /translation="MSTSHDAHADDHGHGHHGSPFVQHHYDDAQHQFDSGKLGIWIFL
                     AQEVLFFSALFVAYILYRVHHGEIYSYAHKWLDVKMGAINTAVLITSSLTAAWAVRCA
                     QLNQRRGLIFCIAATIACACGFLVIKYIEYSHKYHEQILFGRYFDPCETPGGNPLITK
                     SNDCPGRLGTVKWDFGAGKATEGCFESSTIDLDPREPGVQAECEVFEVKLGHDGKPEE
                     SSKKPITNVCAPKIGHGEEWEKNKFPCWMPSYQPAVCPDLTDPAKRAQGAAGRPQVGI
                     IVSYGEDDHEHRPHSIIKAECKKPAPPPAVDLTTEVEHHKLELGRGLAKTHVEEHHGH
                     DLLYAGPPPEHTNMFFTIYFAMTGLHGIHVLVGVFVFIWLLIRAIKGQFTPDYFGPVD
                     YAALYWHIVDLIWIFLFPLLYLIV"
     gene            complement(10867..12861)
                     /locus_tag="VFV99_12760"
     CDS             complement(10867..12861)
                     /locus_tag="VFV99_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX5060229.1"
                     /translation="MATTANRQPDGPVKEPDFLHVTRGLRSWLLTLDHKRIGMMYLCG
                     ILISLIVGGAFALIVRTELWAPGKLFVSGDTYNNFFTLHGAVMVFLVIIPGIPAALGN
                     IIMPIQLGAPDVAFPRLNLASFYLWVAGALLLVLTIPFGSLDTGWTLYTPYSLETKTP
                     VLIAVSGVFLLGFSSIFTGLNFLVTIHKFRPQGMGWFQLPLNVWAIYATAIMQVLATP
                     VLGITVLLLAVERFAHIGIFDPNMGGDPVLFQHFFWFYSHPAVYIMIIPGMGVISELI
                     STFARKPIFGYRFVAYSSVSLALLSFIVWGHHMFVSGQSRLANMVFSALTFTVGIPSA
                     IKVFNWVATLYKGDIRLKTPMLYALGFLLLFTIGGLTGLFLGILSVDVHLHDTYFVVA
                     HFHYVMMGSTLVAFLGALHYWYPKFTGKMYPESLGQLCSLGVFFGFNLTFLPQFVMGS
                     RGMPRRYWDYDPQYQIFHQLSTIGAYVLGISIFISVVSLFWSFKYGKKAPRNPWGGSS
                     LEWQAPTPPTLYNFENPPVLHELYNYDDLEEIEPDVWVRNTPIEDAPARADAIARQTG
                     AVADHTHGASLKEKAISDVHAKAEEVRAEHKLTETSHGRPVDPAKIDQDFKDAAKVSG
                     AGEVMPTAEKVEEVEAKDKPGSVTQGWDVPEVPSDDKEKK"
     gene            complement(12872..14275)
                     /gene="coxB"
                     /locus_tag="VFV99_12765"
     CDS             complement(12872..14275)
                     /gene="coxB"
                     /locus_tag="VFV99_12765"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5060230.1"
                     /translation="MKSIIRCLVLAAFVVAAGSASAQPAPQGSGGQTTTGTAGSAAVA
                     PEKGTAPIVPPVSPADKGAAQGTGPEGAQSPGPSTQPTGSPDGAGGSDAGSAANVGSA
                     AGSAAPAGAGSAAPAGAGSAAPAGGQAGVAWYDVLANKPIEESGTFWMPKSVNEAADD
                     SDLMFYSVLALSAIFFFAITIAVVYFAVKYRHRPGHKPQPSAAHNDALEITWTVIPTI
                     IVVFLFWYGWRSYIRVVTPPQKAVEIQVLAWRWNWQFTHANGVTDSDLHVPVNTPVRL
                     VMTSKDVLHSFYAPAMRVKQDLVPRRYTYAWFKATKPGTYRLTCAEYCGTSHAQMACL
                     ETDKHTGSCLRRAVVVVHSPGDYERYLADKAANMLNMAPVDLGKLVYEKKGCNACHTV
                     DGSARVGPTWKGSFGTTVPLSDGTSAKVDENYIRESMMAPQAKLHQGYPPSMPSFEGQ
                     LKEKEIEGVIEYMKSLK"
     gene            complement(14272..15180)
                     /locus_tag="VFV99_12770"
     CDS             complement(14272..15180)
                     /locus_tag="VFV99_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5060231.1"
                     /translation="MIRNALLALAVMASPVLAHPGEQAAPESGGDEILAPPPTYKGNE
                     VKVDEKLGAKIPVDARFRDQDGKVVTLGELLAQGKGGAETLPTILTFNYSDCPMLCNL
                     QLNGLTAALPKIADKTSDGMFRLGAQFRIVTIDLEPNDTLDKLSRMRARYIDRLPEAQ
                     RAAAQTGWTFLLAETPGDGAAIRRVADAVGFSYTYIADRAEWAHPAALIFLSVKGTVT
                     RYVYGIEFEPQVMRESIIKAGTSEPATAVGFMNRCYHYDPDAKNYSHAGVMALRIGAA
                     GFVVLLLSLFGVMHLIRRSRPGVNRA"
     gene            complement(15236..15970)
                     /locus_tag="VFV99_12775"
     CDS             complement(15236..15970)
                     /locus_tag="VFV99_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060232.1"
                     /translation="MSGPKRNPDKLNTIGVVVVGICGAVLVYVTIVALQAFYMNNTSE
                     IQTMADYGGQDLTAKGRKADEMRNITEAGKNSVGPGQEATFRIPIQNAMQKIVEDAKA
                     DPSHLIPALPPSTKPSIEPVFGRPKALGAAPAPEAGSAAGSAAEPPSVPMSPTGAPTG
                     PQPVPGAAGAAGAATPTPVPTNVPQMTTTPQPGQAPKAGGAAQPKAPAQPKADAKAPA
                     QPKADAKAPAQPKADAAKGSAQPKTP"
     gene            complement(15980..17272)
                     /locus_tag="VFV99_12780"
     CDS             complement(15980..17272)
                     /locus_tag="VFV99_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060233.1"
                     /translation="MSALTKTLDPNEKVKAGKLGSNMVKAGIAIFVVFGVISVVLGGL
                     QGDHWRRFMYSYVIGWSFIVGISIGMLWLVLLHHLVRGRWATVVRRLAEAMTQAFPLI
                     FIAGLAFVIPVVAGYGDLYYWAHPDAKIAALNPHMQHKLGWLSPGFFAIRYVVYGVIF
                     TGMAAYFARKSRQQDESGDPKISDQLRIASGPGMILFALTTCALAFDVLMSLAPKWYS
                     TIYGVGFWASACVSAFSVLALVVLWVQRNGRLVHSVNEEHYHDIGKWMFSFTFFWAYI
                     AFSQFMLQWYGNMPEETVWYKYRMFGDWQWVSIAILVGYWAFPFVFLMSRWTKRIRPS
                     LVFFAVWQIGFTWLNLYWNVMPQYDWATTYEGTTKLVQGPLMGPLAEHHVGLSPVDFT
                     VWIALIGVLLIGIGRNLTGNLIPVKDPTLGLSLAHEQL"
     gene            complement(17269..18561)
                     /locus_tag="VFV99_12785"
     CDS             complement(17269..18561)
                     /locus_tag="VFV99_12785"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX5060234.1"
                     /translation="MSTTHDTPATEPVNGDGELYGVLAEFDTPGELVEAARRVRDAGY
                     TEFDCYSPFPVHGIDPAMGIKRTILPAVIFGGGMAGALGGLLLQWYCNSYAWTWNISG
                     KPLWSIPANIPIAYELTILISAITAFLGMWAFNKLPQVWHPFFRKDRFLRATDDGFFV
                     GIEARDRRFDLEKTKQLLQDAGAIAIEPAYYDADPAKKVMPKWITAFIVASTAFALIP
                     FAIAAKARNSHSSEPHIHIFPDMDFQPKYRSDQPMDVFQDGRANRGEIPGTVARDNLQ
                     ADDVFYHGLEGGDGAPKWTTAFPKTYPDGRPFNVDMALLDRGKNRFEIYCTPCHGYDG
                     RGAGMVPERVKAGGGLWQARNLVEAPDAKGNGGVVVQMPNGQLFNTISNGYNTMMGYA
                     SQIPHADRWAIVAYVRALQRAQNASQADVPPDLQGSIR"
     gene            complement(18561..19952)
                     /gene="nrfD"
                     /locus_tag="VFV99_12790"
     CDS             complement(18561..19952)
                     /gene="nrfD"
                     /locus_tag="VFV99_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5060235.1"
                     /translation="MSETAAAVHEPGKAREAFRSITRDVSWIAEAPKPHKLWLVALAI
                     SVCMFGIGLYSIYVLVTTGIGVWGNSNTVAWAWDITNFVWWIGIGHAGTLISAVLYLT
                     RQQWRVSINRAAEAMTIFAVLCAGLFPLLHTGRPWFAWWMLPLPNDMGSMWPQFRSPL
                     MWDVFAVSTYLTVSLLFWYMGMVPDLATFRDRCSLQGKKIRSAIYGVLSLGWRGSARH
                     WHRYERAYLILAGLATPLVFSVHSVVSFDFATSQLPGWHTTIFPPYFVAGAIFSGFAM
                     VLTLLLPLRFLFNLQHIIKQEHIHAMCRIMLATGMMVGLAYATEFFIAWYSGNPFESF
                     TFISRAFGPFWWAYFMMVSCNVLVPQFFWSKWVRNNNILIWVLCIFINIGMWFERFVI
                     IATSLARSFLPSAWSYYHPTRYDIGLFIGTIGMFVMFFLLFFRFLPVAAMSELKGASP
                     EAHAGGPSKGGHH"
     gene            complement(19956..>21363)
                     /locus_tag="VFV99_12795"
     CDS             complement(19956..>21363)
                     /locus_tag="VFV99_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5060236.1"
                     /translation="WLWETPDFLTKITWDNYALVSPETSVALGVKNDQMITVKVGDKS
                     ITLPCYTMPGQAKYSIGLVLGAGRTHSGRAGATGGLRSGINSPGWDTYKVRSSTGFDY
                     AGSATVTGTSNTYQLANVQDHWNYKPGSNRLMSNDDSAGLRFDHEVERRAHDLVEEIT
                     GEELAKDKAWKAESEAEFWDDAIVQEAKGQKATHLSLFEEHDYGKHHRWAMAIDLTTC
                     TGCNACMVACQSENNVPTVGRREVINNREMHWIRIDRYFTGAPEEPHVVHQPLPCQQC
                     ENAPCEQVCPVGATSHSEEGLNDMAYNRCIGTRYCANNCPYRVRRFNFLDWNKEYREA
                     RQKVRRLLFNPDVTVRMRGVMEKCTFCVQRIQNGKIRAKAELRNPTPSTDRTGAIQGP
                     LPDGQIETACQMACPTEAIVFGDLLDPASRVSKLHADRRAYDLLPEMYTKPRNRYLTR
                     VLNPHPKMPQPPKAQGEH"
BASE COUNT         3727 a         6865 c         7169 g         3602 t
ORIGIN      
        1 ctcgaacacc tcgtggaagt gggtcgtgaa cttattgatg acctcgtccc gcgtcttgtc
       61 gtcggcatcg ggtagcgcca actttgcgcg ctgagtcacg agctggcgga tatagatgcg
      121 gacaaacgtc ttctcggtga agtcgaaatt gccgtcgatg tacccgaacg cggtcagata
      181 aaagatgatc gcgttcatct gctgttcggc gacgttgggg tctcggctga acgtgagcat
      241 cggtgttgcg atcctgcctg gatcgcgcgc ggcgctccag gctgcgatat gattatttgg
      301 tgcgcgccgg ggtcctcgcc acgctcgtgg ctttatcgtc gcctgccatc gcagatcctg
      361 cagatcggct cgaggccagc ggcttcctcg gcgtcgagga tttcagcaag gagaacgggc
      421 tcggcaacgc gctcgcgccg gaacaacggc cacagacggc gccgacgttc ggcggccggc
      481 tgacctactt cgcgctcgag acgagcggcg acatgcacct cgatctcggc gtcgaagccg
      541 agctgtcgtt cacgccggcg tggaccggct acggtttcaa ggaagcccgc ccgtcgtact
      601 tcgcgccggt gtttggctac cgcgcgaact tgctgcttcg gctcggtggc ggttggttcc
      661 aaccgcacgt cagtggcggc gtcggtgcgc ttaccgtcgc gtcggactcg ccgctgatgg
      721 cgaaggagac cgacccggtg ttcgtgtggg gtctcggcgc gcagttcgcg atgggcagcg
      781 gttggcaagt gcgtctcgac gcgcgccagg cgctcaccga gtcggtgacc ggtgacacga
      841 cgtcgaacct cgaagcgctg ctgtcggtcg gcatgcggtt cggcgagaag ccgccgaagc
      901 cggagcccat cgagcacatc gatgtcgtcg tgcagaagcc gccgccaccg cccgagcccg
      961 acaaggacag cgacaacgac ggcattccgg acaagctcga cggctgcccg gcgcaagccg
     1021 agtccgtgaa cggcatcgac gatcaggacg gttgccccga gccggatccc gacggtgaca
     1081 agatcgtcgg gaccgccgac aagtgccccg atcgcgccga ggacttcgac aagttcgagg
     1141 acgaggacgg ctgtcccgaa gacgacaacg acaaggacgg catcgtcgac gcacgtgacg
     1201 tctgcccgaa cgagcccgag acgaagaacg gcatcaccga cgacgacggc tgccccgatg
     1261 cagtgccgcc tgcggtggtc gaagcgctcg cgaccgcaac caaggcgaag ttcgatgcga
     1321 acagcgtgcg catctcgtcg cggatcaaga ccgcgctcga caaggcgctg atcacgatga
     1381 tgagcaacgc gaagctgaag tacgtcatca ccgtccatcc cgacaaggac ggcgacaagg
     1441 acgcggtgct cgcgaatcgc cgcgccgaga acgtgaagtt ctacctggtc gagcaaggtg
     1501 tcgcgatggg cgcgctgtcg acgaccgtcg gcccggtcag caaagacaag aacgcgcccc
     1561 agctcgagat cacgatcgcg ccgtagcgtg atcggtctcg tcgccaagct gctcgaagcg
     1621 ccgctcgcgg tcggcgacac gtcgttcgaa gcgcgcgatg cgatcgtcgt gctcggcgca
     1681 ccgctgacgg cggcgggcga gttgtcgcag gtgctcgaag agcgcgtcgc cgcagcagtc
     1741 gcactctatc gcatgggtgg tgggcggctc gtcgtcgcga gcgggggccc gacgcacggc
     1801 acgcgcccgg aggccgccgc gatcgcggag gcgttgcacg cagccgggat ccccgatgtg
     1861 ctcgtcgagg atcgctcgct gtcgacggca gagaacgcgc ggctcaccgc cgaggtactc
     1921 gcgccgctcg gcgtccgcac ggtgtggatc gtgacgcagc cgtttcacgc gaagcgcgct
     1981 gcgcggctat ttcggcgcgc cgggctcgac gcgcgcagct ggcacatcgc ggacagcgtg
     2041 cagtatcgcg atcgcgggcg cgcggtgcgc tggctcgtcc gcgagtacgc gtcgtgggcg
     2101 gcgctcgcgg tgcgtggccc gcggtagggc gtacacgtgc tcgtgctcgt gctcgtgcgg
     2161 cgctgcggat gacggattgg gcgctgaccg aaccgtcgcc cctgcggaat cgccgtcgaa
     2221 gacggacggg aggtatcacg ccgggctcga ggcgcgccat tgcaggcgcc aacgcgacct
     2281 cccgcagacg gggatgccgg ctcgcagccg gtgaatcgct tgccctgcgt caccaccagc
     2341 tcccgagcac gtctacgtgc acgtgcacgt gcacgagcac gattacggcg cgtcgacgcg
     2401 atccggcagg tcggcgacga cgtacgcgag cacggcgacg gccgcgacca tgtcggcgag
     2461 gtcttgcgga tcgaccttgt cgagcgtgtc cgcctcggtg tggtgatagt cgaagtactt
     2521 ctcgccggcg acctcgaggc cgacctgcgg cacgcccgct gccgccatcg gctcgatgtc
     2581 ggtgccgccg tgtccgtcgt cgaccttcag cttgccgagc ggcgcgagca acgcagcgac
     2641 ctcggtcagc cgcgcacgcg tgcgcttcgc ggcctcggga tctttgtggc cgacgccgaa
     2701 gccgcgcggc gcgaagccgc cggagtctga ttcgagcgcg agcaccgtct tcgcgagctc
     2761 ggccttgtgc gtctcggcgt aggttttcgc gccgcgtgtg ccgttctctt cgttcgtgaa
     2821 cagcacgacg cgaatcgtgc gccgtggcgt gaggccgagc tgcttgagca cgcgcaccgc
     2881 ctgcatcatc gtgacgatgc ccgcgccgtc gtcgtgcgcg ccctgcccga cgtcccatga
     2941 atcgatgtgg ccgccgatca cgaccacttc gtcgggtttt tcgcggccgc gcagctcgcc
     3001 gatcacgttc gccgattcgg catcgggcag cagttgcggc tcgagtcgca aatgcagcgt
     3061 gaccttgccc tgtgcgctca accgatcgag cagctcggca tcctcgacgg tgatcgacgc
     3121 ggtcgggatc ttgggctgtt ccttgtcata acccatcgcg ccggtgtgcg ccgtcgacaa
     3181 gctcttcgcg gtcaccgaac gcacgagcgc cgcgacggca ccacgctttg cggcttgcga
     3241 tgcgcccgcg aaccgatact cgacggtctt gccgtagccg gagcccttct cgggcgtcca
     3301 cgccggcatc gcgacgttgt agacgacaat cgcgcccttg accgactcgg ctttcgcgtc
     3361 gagctccttc cagtcgtgaa cgacgacgac cggcgcggtg ataccgccct tgggcgtcgc
     3421 gacgctgccg ccgagcgtga gcaggtgcaa ggggcgcgtg atcggcgcgg tgatcgatgc
     3481 ttcttcggcg ccacgcaccc agtgcggcac catcaccttc tcggtacgcg cgtcgaggcc
     3541 gtcgtctttc atcgcctgcg ctgcccacgc gatcgccttg tcgagctccg gcgagccggc
     3601 gagtcgatgg ccgatctgat cggtgaggta cgcgagcttc ttgtacgcgc cgcgatccgc
     3661 gcgcgcggtc gaaatgatct tgtttgcggt ctcgcgataa tgctcgacga tcggacgcgg
     3721 ctggttcggg ccgccgtacg agctcgtgtc gttgacccac gcgggcgtgg gtgcttccat
     3781 caccagcggt gcgcagccgg cgagtgtcgc gagtactgct agcgctttca cgacgaggac
     3841 tctacgtgat cgagcgccgt cggggcagcg gcaggctcga ccgggacccc acgcgcggcg
     3901 atatcgacta acagctcgat aatcgcaatt agccgttccg ggctggccgt gcggccgagc
     3961 gcgaggtccg agagcggcat cgggtggtcg agcggcgcgc cgcgacctgg atacacgcgg
     4021 tagcgaaggc cttccggctc ccagtaggcg agccagccat cgagcgtcgc gatgacacgc
     4081 gctcgtagcc cctcgtcgag cagcttgtcg aacgcgacgc gattgccgcg cgtcttgaac
     4141 cgctgatcga acgtcgtgtc gttcgtcttg atgatcggcg cagccggtgg gccggccggg
     4201 ttgatgccga gtgtacgggg cggaatcgcc cacagcgtga gcgtcgcgcc gcgcagctcg
     4261 tcgatctcgc ggccaatgat cacgtcgagc gcgatcacgc tgccttcgat gcgctcgatg
     4321 cgcgtgcgca cgttgagccc atccttgtcg ccgataatca ccgacgatgc gagcccgccg
     4381 tcgccgcgcg tggtcagcgc atgcacgccg gcgagcgagc gttcgagccc ggacttcacg
     4441 ctagcgacgt aaccggacca tgcggcatcg gcgatcggcg gtgtatcggt cgtgatcggc
     4501 agcgcggcga gctcttcctc gcgcaccttg cgcaccgccg tcgtcacgag cgcgatgccg
     4561 agcagcggca acaggtagtg attcggccag cgaaacgcat agccgccgag gatcacgaac
     4621 gcgatgccgg caccgatcgc acggcgcgcc tggctatgcg cgatcgcgca cgacgcgagc
     4681 gtccacgcca gcgtcgcgat cgcgagcagg tagatcgcga gacgcggatc ggcctgggcc
     4741 tggttgagct cgacgccgac cgcgagtgcc gccgccttcg cgacgatcgg ataccaagtg
     4801 cgtgcggcga ccgcgccgag cgccgcgatc gccatcgcga tcacgaccgg ccccggcctc
     4861 gtcacggcgc gcgcaaacgg ccgcggcgcg aacacgtacg gcgataccaa ggccgcggcg
     4921 cacagtgcca tcacgcccgc gcgcgtgatc tcgacgccgg gaccatcgaa cgcgccctcg
     4981 ggccacaggt acttgccgcc gagcaccgtg accgtgtgca acacgagggg cagcgagagc
     5041 gtgacgacac cgagctgcgc accgacgtcg tagtggtggc tgatgctcgt cgagaacatc
     5101 gcgatgatcg caacggcgaa cgcgacctcg agcgcgaaca cgaggctcgg tggcgcgcca
     5161 atgacgagcg ggatggcagc gagcacggtc gcgactgcga gcgcgccgtt cgcgatgaag
     5221 tcgcgcacgc tccgtgacgt ccgcagtccc tcgacgacgc gcacgccgat caacaccgcc
     5281 gcgagcgtgc cggcgaagta gaacaggaac agcccgccgt aatcgagcgc ggtgtgccac
     5341 gtcggcggcg tacctttcgc cggtcgcaac atcggcacgg cgacgcgatt gatcgcgacc
     5401 tcgaggaaag cgaccagcag gaggatgtga atgggggcaa gaccccctgc aaggcgccga
     5461 cgagacaacc ggaaaaacgt agcgttgtgg actatttaaa tcaaggagcg cgaaagttcg
     5521 ggacctgctt tcaggtaagg cgcacgaaaa gttgggcacc gcgctcccgg cacgagaaaa
     5581 ttttctctgt gaggttttgc ccgagcttgc tcgtgctggt cggcctggcc gcctcggccg
     5641 ccacgtcggg ggccgcgccg tccaggccgc cggtcgtcgt gctcgatccc ggccacggcg
     5701 gctcgaaccc gggcgcgcaa ggcgtcgccg ggcttcgcga aaagcagctc acgctgaccc
     5761 tggcaaacct ggtcgcgacc cgacttcgcg agcatggcat cgccgtcacc ctcacccgcg
     5821 acaccgacaa gaccctgacc ctccgccagc gcgtcgaggt cgccgacaga ctcccagccg
     5881 acatcttcgt cagcatccac gcgaacgcgt caacgacccg ggcgcaaagc ggctacgaga
     5941 cctacgtgct cacggcgaag ggtgtcgata tcgacgcccg tgcgctgcgc agcggcacca
     6001 cgacaccacg acccggcgtc gatcccgaca tcgcactcgt gctcgatgat ctcgagcgcg
     6061 gtgcatcgca atgggaatcc gccgagctcg cggcgcgcat gcaacgctcg ctgcgcgatc
     6121 gccgcggccc cgatggcgat cgcggcgtgc gccaagactc gcaccacgtg ctgctcggcg
     6181 cgacgatgcc ggccgtgctc gtcgaggtcg gcttcatcga tcatcccatc gagggcaagc
     6241 agctcgccga tccgcagatt caagccggca tcgccgctgc catcgccgaa gcgatcgaag
     6301 atcagctcgc cgactgatta ttccgccgtg aacgcggcgg cgatcgagta gttagcgcgt
     6361 ggaacatggc cgaggctgcg cgcgttcttc gcagcacgat gaaacaaatc ctattggccg
     6421 tctgtcttat cgctactgca tcgcctgcgt tcgccgacaa gaaagccgac gacaccgcag
     6481 ccgcctccga agcgaacgtc tccaccgagg tgaaagccgg caccggcgtc gagaaccacg
     6541 aggtcgtcgg cgaagcgacg acgttcgagc agggcacgac cgtgttcgtg tggtcgcgca
     6601 tcaccgacgg cccggcgagc atcaagcacg tgtggaagct cgacggcaaa ccggtgtgga
     6661 cggcgacgct gccggtgaag tcgaagaagt ggtcgacgca gtcgcgccgc gtgatctcga
     6721 agccgggcgc ctacgaagtc gacgtgacga gcgccgacgg cgtgtcgctc ggcacgatcg
     6781 cgttcacggt caccgcgaag taatcagaag cggcccgcga ggccgacggt cgcgccgccg
     6841 ttcgccggcg ccacgtacgg atcgccacat tgtcgctctc gcgcggtgcc gaggaaacct
     6901 tctagagcgg atcccacgtc gggccaccgc gccagtacac gcccatcgcg cggccgagaa
     6961 acgcgtcgcg gctgacgagg ccaaacgatc ggccgtcgtg actgtcgccg cggttgtcgc
     7021 ccatcacgag atatttgccg tcgggcagcg tcaccggtcc gaaatcagga ccgccggcgt
     7081 tggtcagccg caccaggtgg tgcgtgcctg cgaggtcttc gacgagcatg ccgtagtgct
     7141 cctcgatcgg cgcgagcttg ccatcgatcg agatctggcc gagatgcacc tcgatggtcg
     7201 tgcccggaat gccgacgaca cgcttgagca agacgcgccc gtcctcggga gagcgcagca
     7261 cgaccgtgtc gccgatctgt gggccttgga actcggtgag gaagctgctc gtgaacggca
     7321 cgcgcaaccc gtacgcagcc ttgttgacga ggatgcgatc gtcgatgtgg accgtcggga
     7381 gcatcgaacc cgtcggcact tgatagtgat cggcgagcga cgagcgcgcc gacagcagca
     7441 cgaacgcgag caccatgaag accgagatct cgcgcaccca gcggcgcagt cgcgatcgct
     7501 tcggtgcggg agagtcggca ggcgacgcgg cgtcagacgg cgtatcggtg gacgtgtcgc
     7561 taggcgtggc gtcagtcaca gcacgtcgac cagcgtcgcg cgcggcgtta ttcctgctac
     7621 gacctccgtg ccctggtccc tcgaaaatct gtggccactg gccgcgcaac tcaccgcaat
     7681 caggcgcttg gtgctggcct gcaccgtgaa tggttggacc agccatgtcg tggcttaccg
     7741 tcatcgtgct cgggcgcatc ctggcagaga gtggctcgcc gccacctgac gtcgacgacc
     7801 agacgatcct gctgatcaag cgcaaggacg tgatggtggg ctgctcgccc ggcgatgccg
     7861 gtgtggacat ggacgctgcg agcgacgcac ccgacggcga cgccgcgatc ggcgatgctg
     7921 cgctcgacga tgccggtgtg agcgacgccg gtatcggcga cgccggtgtc gacgatgccg
     7981 gcgtcgacgc gagcacctgc acgatgatct ccggcgacgc ggtcacgctg gtcgtccagc
     8041 cgcacgtgtc gacgagcgcc gacggcaccc gcttcgcggt cctgctcgtc acaccggacc
     8101 ggccgatcgt cgagcttgcc ggcgacgtat tcagcagcct cgcgcagacg acggcgccgc
     8161 gcatcgacat ccatacggtc gaggtgcccg acccggcgct cggccagcag tgcgcgacct
     8221 acgagtcggg atgcggcggc ggcgagagcg atggctgcgg cggtgacttt ggcgatgacg
     8281 gctcgtcgtg gacgccaccg aagatcggcg acgcggggct cggcgatggc gggctcgtgc
     8341 aagagatgat cgggccatat cagttcgtgc gcgcgcagcc gacgtcggca atggagctcg
     8401 cgggctggct cgatcaattc ggctacgcgt acatgcagaa ggatctcgac gcggtcgcgc
     8461 cgtacatcgc gctcggctat cacgtcgtag cgattcgcgt gtcgctcgat catccgctta
     8521 ccgcgccgat gattccgatc tcgctgacgt gggcaggcag cgaactgcgc gtgccggcgg
     8581 cactcggcgc cggtgctgtc gggaccggca cgctgacggt gtacatcgct gccgagggca
     8641 agtatcagtt cccgaatgcg acgatccggt tcgcgcggca aacgagcgcc ggcggtgcgg
     8701 gctacctcac gcgcaaccag atcacactcg atcagaacct gccgcccgag caggatccga
     8761 tcgcatatcg cgtgaccaat agtgacttcc aggaggtcac gacccagacc gagtacgtgc
     8821 acgtgcccgt cgacgtcgat tgctcgcagc aggaagagcg cggcgggtgc tgcaacgagt
     8881 gcaacgcgaa accacgcaat cgcctcgatc tgctgacgat cgtggtcgca gtcgcgttcg
     8941 tgctgcgccg ccggcgccgc tagtcagagc ttctcgcgcg tacgtggtcg tgcgccacgc
     9001 tgtggcagcc gagcgccgac ggaacaatcg gcaaggacgt tgcacaacgt agagtcatgc
     9061 tgactctact tattgttctc ttggtcctcg cgctgctcgg tggcggactt ggctacgggc
     9121 gcttcggcgc agtgggcatg tcgcccgcgg cgctgctcgt gatcattctc atcgtgctgg
     9181 cgctgatggg tcgcctgtag gcccggcggc ctcgcgagct agccgttgat cgcgcgcgga
     9241 ccgaccggcg ccatcggcgg agccgtcgga ttccagatgt tcgtctgctg gtactggtcg
     9301 gtgtccatca gcgtgaagcc gacgaacagt gtggcggcca atatcgcact acagaacacg
     9361 accgagtgga acaaccggtc gtagcgaagg tgcatgaaga agaggacgac gagcgtcgcc
     9421 ttcgtcactg cgatcaccat cgcgatcgcg aggttgatgc tcgcaccgcc gaagtcgatc
     9481 ttcgacgaga tgaccgtgac gagcgtgagc aggagcaagg tgccgccggt agcgaggagc
     9541 accttgatcg ctgcgacgtg agcgaggcca tgaccgtggt cgtcgtgccc gtgatgtgga
     9601 gcggttgcca tgatggacct cagacgatca ggtagaggag tgggaagagg aagatccaga
     9661 tcaagtcgac gatgtgccag tacagcgcgg cgtagtcgac cggtccgaag taatccgggg
     9721 tgaactgccc cttgatcgcg cggatgagca gccagatgaa gacgaacacg ccgacgagca
     9781 cgtggatgcc gtgcagaccg gtcatcgcga agtagatcgt gaagaacatg ttcgtgtgct
     9841 cgggtggcgg tcccgcgtac agcaggtcgt gcccgtggtg ctcttcgacg tgcgtcttcg
     9901 cgaggccccg accgagctcg agcttgtggt gctcgacttc agtggtcaga tcgaccgccg
     9961 gcggcggcgc gggcttcttg cactcggcct tgatgatcga gtgcgggcga tgctcgtgat
    10021 cgtcttcacc gtacgagacg atgatgccaa cctgcgggcg accagccgcg ccttgcgcgc
    10081 gcttcgccgg gtcggtgagg tcggggcaca ccgcgggttg gtacgacggc atccagcacg
    10141 ggaacttgtt cttttcccat tcctcgccgt gcccgatctt cggcgcacac acgttcgtga
    10201 tcggcttctt cgacgattcc tcgggcttgc cgtcgtggcc gagcttgacc tcgaacacct
    10261 cgcactcggc ctgaacgcca ggttccctcg gatcgagatc gatcgtgctc gactcgaagc
    10321 agccctcggt cgccttaccg gcgccgaagt cccacttcac ggtgccgagc ctgcccgggc
    10381 agtcgttgct cttcgtgatc agcggattgc caccgggggt ctcgcacgga tcgaagtagc
    10441 ggccgaacaa gatctgctcg tgatacttgt ggctgtactc gatgtacttg atgacgagga
    10501 agccgcatgc gcacgcgatc gtcgcggcga tacagaagat caggccgcgg cgctgattga
    10561 gctgcgcgca gcgcacggcc catgcagcgg tgaggctcga ggtgatcagg acggcggtgt
    10621 tgatcgcgcc catcttcacg tcgagccact tgtgcgcgta gctgtaaatc tcgccgtggt
    10681 gaacacggta gaggatgtag gccacgaaca gcgcggagaa gaacagcacc tcctgcgcga
    10741 ggaagatcca gattccgagc ttgccggaat cgaactggtg ctgagcgtcg tcgtagtggt
    10801 gctggacgaa cggcgagccg tgatggccgt ggccgtggtc gtccgcatga gcgtcgtgcg
    10861 aggtgctcat ttcttctcct tatcgtcgga gggaacctcg ggcacgtccc agccctgcgt
    10921 caccgagccc ggcttgtcct tcgcttcgac ttcttcgacc ttctccgcgg tcggcatcac
    10981 ctcgcctgca ccggagacct ttgccgcatc cttgaagtcc tgatcgatct tcgccggatc
    11041 gacggggcga ccgtgcgacg tctcggtgag cttgtgctcc gcgcgaacct cttcggcctt
    11101 cgcgtggacg tcggagattg ccttctcttt caggctcgca ccgtgcgtgt gatcggcgac
    11161 ggcgccggtc tggcgagcga tcgcgtcggc gcgagcgggc gcgtcctcga tcggcgtgtt
    11221 gcgtacccag acgtcgggct cgatctcctc gaggtcgtcg tagttgtaga gctcgtggag
    11281 gaccggcggg ttctcgaagt tgtagagcgt cggcggcgtc ggcgcttgcc actcgagcga
    11341 gctgccgccc cacgggttac gcggcgcctt cttgccgtac ttgaacgacc agaacagcga
    11401 cacgacgctg atgaagatcg agatgccgag cacataggca ccgatcgtcg agagctgatg
    11461 gaagatctgg tactgcgggt cgtagtccca gtaccggcgc ggcatgccgc gcgagcccat
    11521 cacgaactgc ggcaagaacg tcaggttgaa gccgaagaac acgccgagcg agcagagctg
    11581 gccgagcgac tccgggtaca tcttgcccgt gaacttcggg taccagtaat gcagcgcgcc
    11641 gaggaacgcg accagcgtcg aacccatcat cacgtagtgg aagtgagcga cgacaaagta
    11701 cgtgtcgtgc aggtggacgt cgaccgacag gatgccgagg aacagaccgg tgagaccacc
    11761 gatcgtgaac agcagcaaga agccgagtgc gtagagcatc ggtgtcttga gccggatgtc
    11821 acccttgtag agcgtcgcga cccagttgaa gaccttgatc gcgctcggga tgccgacggt
    11881 gaatgtcagc gccgagaaca ccatgttcgc gagccgcgac tggcccgaca cgaacatgtg
    11941 gtggccccac acgatgaacg agagcagcgc gagcgagacc gacgagtacg cgacgaagcg
    12001 gtaaccgaag atcggcttgc gcgcgaacgt cgagatcagc tcgctgatca cgcccatgcc
    12061 cgggatgatc atgatgtaga cggcagggtg cgagtagaac cagaagaagt gctggaacag
    12121 caccggatcg ccgcccatgt tcgggtcgaa gatgccaatg tgcgcgaagc gctcgaccgc
    12181 gagcagtagc accgtgatgc cgagaaccgg cgtcgcgagc acctgcatga tcgccgtcgc
    12241 gtagatcgcc cacacgttca gcggcagctg gaaccagccc atgccctgcg ggcggaactt
    12301 gtggatcgtc acgaggaagt tgaggcccgt gaagatcgac gagaagccga gcaggaacac
    12361 gcccgacacc gcgatgagca ccggcgtctt cgtctcgagc gagtacggcg tgtacagcgt
    12421 ccagccggtg tcgaggctgc cgaacggaat cgtcagcacg agcagcagcg cgcccgcgac
    12481 ccacagatag aacgacgcga ggttgaggcg tgggaatgcg acgtcaggcg cgcccagctg
    12541 gatcggcatg atgatgttgc cgagcgcagc tgggatgccg gggatgatga cgaggaacac
    12601 catcaccgcg ccgtgcagcg tgaagaagtt gttgtacgtg tcgccggaga cgaacagctt
    12661 gcccggcgcc cacagctcgg tgcgtacgat cagcgcgaac gcgccgccga cgatcagcga
    12721 gatgaggatg ccgcacaggt acatcatgcc gatccgcttg tgatcgagcg tgagaagcca
    12781 gctgcgtagg ccgcgggtta cgtggaggaa gtccggctcc ttgacggggc cgtcgggttg
    12841 tctgtttgcc gtggtcgcca tgatcaggtc ctcacttcag cgacttcatg tattcgatga
    12901 cgccctcgat ctctttctct ttgagctgcc cttcgaatga gggcatcgag ggcgggtagc
    12961 cttgatggag cttcgcttgc ggagccatca tcgactcgcg gatgtagttc tcgtcgacct
    13021 tggcgctggt gccatcggac agcggcaccg tagtgccgaa gctgcctttc cacgtcgggc
    13081 cgacgcgcgc cgaaccgtcg acggtatggc aggcgttgca gcccttcttc tcgtagacca
    13141 gcttgccgag atcgaccggc gccatgttca acatgttggc cgccttgtcg gcgaggtaac
    13201 gctcgtagtc gccgggcgaa tgcacgacga cgacagcgcg ccgcagacat gagcccgtgt
    13261 gcttgtctgt ttccaggcac gccatctgcg cgtgcgacgt gccgcagtac tcggcgcacg
    13321 tcagccggta cgtacccggc ttcgtcgcct tgaaccacgc gtacgtgtag cgacgcggca
    13381 cgaggtcctg cttcacgcgc attgccggcg cgtagaacga gtgcagcacg tccttcgacg
    13441 tcatcaccag acgcaccggc gtgttcaccg ggacgtgaag gtcggagtcg gtgacgccgt
    13501 tcgcgtgcgt gaactgccag ttccagcgcc aggcgagcac ctggatctcg acggccttct
    13561 gtggcggcgt cacgacgcgg atgtacgagc gccagccgta ccagaacagg aacacgacga
    13621 tgatcgtcgg aatcacggtc cacgtgatct cgagcgcatc gttgtgcgcc gccgacggct
    13681 gcggcttgtg accggggcgg tggcgatact tgacggcgaa gtagacgacc gcgatggtga
    13741 tcgcgaagaa gaagatcgcg gacagcgcga gcaccgaata gaacatcagg tcgctgtcgt
    13801 cggcggcttc gttcaccgac ttcggcatcc agaacgtgcc gctctcttcg atcggcttgt
    13861 tcgcgagcac gtcgtaccag gcgacaccgg cctggccgcc agcgggagcc gccgagcctg
    13921 caccggcggg agccgccgag cctgcaccgg ccggcgcggc cgaacccgcg gccgaaccaa
    13981 cgttcgccgc cgagcccgcg tccgagccac cggcgccgtc gggcgagcca gtcggctgcg
    14041 tcgacggacc cggcgactgc gcgccttcgg gtcccgtgcc ttgcgcggcg cctttgtcgg
    14101 cgggcgagac gggcggaacg atcggcgccg tccccttctc gggggcgaca gcagccgagc
    14161 ctgcagtgcc agtggtggtt tggcctccgg aaccctgcgg cgcgggttgc gcgctcgccg
    14221 agcccgcggc gaccacgaag gccgccagaa cgaggcaacg gatgatcgat ttcatgctcg
    14281 attcactcct ggtcgactgc ggcggatgag atgcatcacg ccgaagagcg acaacaacag
    14341 aacgacgaat cccgccgcgc cgatgcgcag cgccatgact ccggcgtgcg agtagttctt
    14401 cgcgtcggga tcgtagtggt aacagcggtt catgaacccg acggcagtcg cgggctcact
    14461 cgtacctgct ttgatgatcg actcgcgcat gacctgcggt tcgaattcga tgccgtacac
    14521 gtagcgagtc acggtgccct tgacgctgag gaagatcagc gcggccgggt gcgcccattc
    14581 cgcgcggtcg gcgatgtacg tatatgaaaa gccgacggcg tctgccacgc ggcgaatcgc
    14641 cgcgccgtcg ccgggcgttt ccgcgagaag gaacgtccag ccggtctgcg cggccgcgcg
    14701 ctgcgcctcg ggcaggcgat cgatgtagcg cgcacgcatg cgcgacagct tgtcgagagt
    14761 gtcgttgggc tcgagatcga tcgtgacgat gcggaactgc gcgccgagcc ggaacatgcc
    14821 gtcgctcgtc ttgtcggcga tcttcggcag cgctgcggtc agcccgttga gctgcaggtt
    14881 gcacagcatc gggcagtccg aatagttgaa cgtcaaaatg gtcggcagcg tctccgcccc
    14941 acccttgccc tgcgccagga gctcgccgag cgtgacgacc ttgccgtctt gatcgcggaa
    15001 ccgcgcgtcg accggaatct tcgcgccgag cttctcgtcg accttgacct cgttaccctt
    15061 gtacgtcggc ggcggtgcga gaatctcgtc gccgcccgat tcgggtgcag cttgctcgcc
    15121 cgggtgcgcg agcaccggtg acgccatgac ggcgagagcg agcagagcgt tgcggatcat
    15181 catctggaag acatcgccag ccggagatgg aggccgactg gtgatgcagc gtgacttacg
    15241 gagtcttcgg ttgtgccgag cccttggcgg cgtcggcctt cggttgtgcc ggcgccttcg
    15301 cgtcagcctt cggttgtgcc ggcgccttcg cgtcagcctt cggttgcgcg ggcgctttcg
    15361 gctgcgccgc gccgccagcc ttgggcgcct ggcccggctg cggagtcgtc gtcatctggg
    15421 gcacgttcgt cggcaccggc gtcggcgtcg ccgcgccagc tgcacccgcc gcacccggaa
    15481 ccggctgcgg accggtcggt gcacccgtcg gcgacatcgg caccgacggc ggctccgctg
    15541 ccgagcctgc agccgatcca gcttcgggag ccggtgccgc accgagcgcc ttcgggcgac
    15601 cgaacacggg ctcgatcgac ggcttcgtgc tcggcgggag cgcaggaatc aggtgcgacg
    15661 gatccgcctt cgcgtcctcg acgatcttct gcatcgcgtt ctggatcgga atgcggaacg
    15721 tcgcttcctg acctgggccg accgagttct tgccggcctc ggtgatgtta cgcatctcgt
    15781 cggccttgcg acccttcgcg gtcaggtcct gaccgccgta gtcggccatc gtttggatct
    15841 ccgacgtgtt gttcatgtag aaggcctgca gcgcgacgat cgtcacgtag acgagcaccg
    15901 caccgcagat accaacgaca acgacgccga tcgtgttgag cttatctgga ttgcgcttgg
    15961 gtcctgacat ggcggtctcc tagagctgct cgtgcgccag cgacaggccg agcgtcgggt
    16021 ctttgaccgg aatcaggttg ccggtcaggt tgcggccgat gccgatgagg agcacgccga
    16081 tcagcgcgat ccacacggtg aagtcgaccg gtgacaagcc cacgtggtgc tccgcgagcg
    16141 gtcccatcag cggaccctgc accagcttcg tcgtgccttc gtacgtggtg gcccagtcgt
    16201 actgcggcat cacgttccag tagaggttca gccacgtgaa gccgatctgc cacaccgcga
    16261 agaacacgag cgacgggcgg atgcgcttcg tccagcgcga catcaggaac acgaacggga
    16321 acgcccagta accgacgagg atggcgatgc tgacccactg ccagtcgccg aacatccggt
    16381 acttgtacca gacggtctcc tccggcatgt tgccgtacca ctggagcatg aactgcgaga
    16441 acgcgatgta tgcccagaag aacgtgaacg agaacatcca ctttccgatg tcgtggtagt
    16501 gctcctcgtt gaccgagtgg acaaggcggc cgttgcgctg cacccacagc acgaccagcg
    16561 cgagcacgct gaacgcggac acgcaagcgc tcgcccagaa gccgacgccg tagatcgtcg
    16621 agtaccactt cggcgcgagt gacatcagca cgtcgaacgc gagcgcgcat gtcgtcagcg
    16681 cgaacaggat catccctgga ccactcgcga tgcggagctg atcggagatc ttgggatcgc
    16741 cgctctcgtc ctgctggcgc gacttcctcg cgaagtacgc cgccatgccg gtgaagatca
    16801 caccgtacac gacgtagcgg atcgcgaaga agcccggcga cagccagccg agcttgtgct
    16861 gcatgtgcgg gttgagggcg gcgatcttcg cgtcggggtg cgcccagtag tagaggtcac
    16921 cgtagccggc gacgaccggg atcacgaacg cgaggcctgc gatgaagatc agcgggaacg
    16981 cctgcgtcat cgcctcggcg aggcgccgca cgaccgtggc ccagcggccg cgcaccagat
    17041 ggtgcagcag cacgagccag agcatgccga tcgaaatgcc gacgatgaag ctccagccaa
    17101 tcacgtacga gtacatgaag cgccgccagt ggtcgccttg cagcccaccg agcacgaccg
    17161 agatgacgcc gaacacgacg aagatcgcga taccggcctt caccatgttg ctgccgagct
    17221 tgccggcctt gaccttctcg ttcgggtcga gagttttcgt cagagcgctc atcggatgct
    17281 cccctggaga tcgggcggaa cgtcagcttg cgacgcgttc tgcgcgcgct ggagcgcgcg
    17341 gacgtacgcg acaatcgccc agcgatccgc atgtgggatc tgggacgcgt agcccatcat
    17401 cgtgttgtag ccattcgaga tcgtgttgaa gagctggccg ttcggcatct gcaccacgac
    17461 gccaccgttg cccttagcat ccggcgcttc gacgaggttg cgcgcttgcc acaggccacc
    17521 gcccgccttc acgcgctcgg gaaccatgcc ggcgccgcgg ccgtcatagc cgtggcatgg
    17581 cgtgcagtag atctcgaagc ggttcttgcc gcgatcgagc agcgccatgt cgacgttgaa
    17641 cgggcgcccg tcggggtacg tcttcgggaa tgccgtcgtc cacttcggcg cgccgtcccc
    17701 accttcgagg ccgtgataga agacgtcgtc ggcctggagg ttgtcgcgtg cgaccgtgcc
    17761 gggaatctcg ccgcggttcg cgcggccatc ctggaacacg tccatcggct ggtccgatcg
    17821 gtatttcggc tggaagtcca tgtccgggaa gatgtggatg tgcggctcgg acgagtgcga
    17881 gttgcgcgcc ttcgccgcga tcgcgaacgg aatcagcgcg aacgccgtgc tcgcgacgat
    17941 gaaggcggtg atccacttcg gcatcacctt cttcgccgga tccgcgtcgt agtaggccgg
    18001 ctcgatcgcg atcgcgccag cgtcttgcag cagctgcttc gtcttctcga gatcgaagcg
    18061 gcggtcgcgc gcctcgatgc cgacgaagaa gccgtcatcg gtcgcgcgga ggaaccggtc
    18121 cttgcggaag aacgggtgcc agacctgcgg cagcttgttg aacgcccaca tgccgaggaa
    18181 ggccgtgatc gccgagatca agatcgtgag ctcgtaggca atcgggatgt tcgccggaat
    18241 tgaccacaag ggcttaccgg agatgttcca ggtccacgcg tacgagttgc agtaccactg
    18301 cagcagcaga ccgccgagcg cgccggccat gccaccgcca aagatgacgg ccggcaggat
    18361 cgtgcgcttg atgcccatcg ccggatcgat cccgtgcacc gggaacggcg agtagcagtc
    18421 gaactcggtg tagccggcat cgcgaacgcg acgcgcagct tcgacgagct caccgggcgt
    18481 atcgaactcg gcgagcactc cgtagagctc gccgtcacca ttgaccggct cggtggccgg
    18541 cgtgtcgtgg gtggtcgaca ttagtggtgc cctcccttcg acggaccgcc tgcatgagcc
    18601 tcgggcgaag cacccttgag ctcactcatc gcggccactg gcaggaagcg gaagaacagc
    18661 aagaagaaca tgacgaacat gccgatcgtg ccgatgaaca ggccgatgtc gtaccgcgtc
    18721 gggtggtagt agctccacgc gctcgggagg aacgaccggg cgagcgacgt cgcgatgatg
    18781 acgaagcgct cgaaccacat gccgatgttg atgaagatgc agagcaccca gatcaggatg
    18841 ttgttgttcc ggacccactt gctccagaag aactggggca cgagcacgtt gcagctcacc
    18901 atcatgaagt aggcccacca gaacggaccg aacgcgcgcg agatgaacgt gaagctctcg
    18961 aacgggttcc ccgagtacca cgcgatgaag aactcggtgg cgtaggcgag accgaccatc
    19021 atgccggtcg ccagcatgat ccggcacatc gcgtggatgt gctcttgctt gatgatgtgc
    19081 tgcaggttga acaggaagcg cagcggcaac agcagcgtga gcaccatcgc gaagccggag
    19141 aagatggcac cggcgacgaa gtacggcggg aagatcgtcg tgtgccagcc gggcaactgt
    19201 gacgtcgcga agtcgaacga cacgaccgag tgcaccgaga agaccagcgg cgtcgcgagg
    19261 cccgcgagga tcaggtacgc gcgctcgtag cggtgccagt ggcgggcgct gccgcgccag
    19321 ccgagcgaca gcacgccgta gatcgcgctg cggatcttct tgccctggag cgagcagcga
    19381 tcgcggaacg tcgcgaggtc ggggaccatg cccatgtacc agaacagcag cgacacggtg
    19441 aggtacgtcg agactgcgaa gacgtcccac atcagcgggc tgcggaactg gggccacatc
    19501 gagcccatgt cgttcggcag cggaagcatc caccacgcga accagggacg gccggtgtgg
    19561 agcagcggga acaggcccgc gcacaagacg gcgaagatcg tcatcgcctc ggcggcgcgg
    19621 ttgatgctga cgcgccattg ctgccgcgtc agatagagaa cggcggagat cagcgtgccg
    19681 gcgtgaccga taccgatcca ccagacgaag ttcgtgatgt cccaggccca ggcgacggtg
    19741 ttcgagttac cccaaacacc gataccggtc gtgaccagca cgtagatcga atagaggccg
    19801 attccgaaca tgcatacgga gatcgcgagc gcgacgagcc acaacttgtg cggcttcggc
    19861 gcttcggcga tccacgagac gtcgcgggtg atcgaccgga aggcttcgcg agccttgccg
    19921 ggttcatgaa ctgcagcggc ggtctcactc atagtttagt gctccccttg ggccttggga
    19981 ggctgcggca tcttggggtg tggattgagc acgcgagtca ggtagcggtt gcgcggcttc
    20041 gtgtacatct cgggaagcag gtcgtaggcg cggcggtcgg cgtgcagctt cgacacgcgg
    20101 ctagccggat cgagcaagtc gccgaacacg atcgcttcgg tcgggcacgc catctggcac
    20161 gcggtctcga tctgtccgtc gggcagcggg ccctgaattg cgccggtgcg atcggtcgac
    20221 ggcgtcgggt tgcgcagctc cgccttcgcc cggatcttgc cgttctggat gcgctgcacg
    20281 cagaacgtgc acttctccat cacgccgcgc atacgcacgg tgacgtccgg gttgaacagc
    20341 aggcggcgaa ccttctggcg cgcctcgcgg tactccttgt tccagtcgag gaagttgaag
    20401 cgacgcacgc ggtacgggca gttgttcgcg cagtagcgcg tgccgatgca gcggttgtag
    20461 gccatgtcgt tgaggccttc ttcgctgtgg ctcgtcgcac cgaccgggca cacctgctcg
    20521 cacggcgcgt tctcgcactg ctgacacggc agcggctgat gcacgacgtg cggctcttcc
    20581 ggcgcgccgg tgaagtaccg gtcgatgcga atccagtgca tctcgcggtt gttgatcacc
    20641 tcccggcggc cgaccgtcgg gacgttgttc tccgactgac acgcgaccat gcaggcgttg
    20701 cagcccgtgc acgtcgtgag gtcgatcgcc atcgcccagc ggtggtgctt gccgtagtcg
    20761 tgctcttcga acagcgagag gtgcgtcgcc ttctggccct tcgcttcctg gacgatcgcg
    20821 tcgtcccaga actccgcttc ggactcggcc ttccacgcct tgtccttcgc gagctcctca
    20881 ccggtgattt cctcgacgag atcgtgagcg cggcgctcga cctcgtggtc gaaacgtagc
    20941 cccgccgagt cgtcgttcga catcagccgg ttgctgcccg gcttgtagtt ccagtggtcc
    21001 tggacgttcg cgagctggta cgtgttgctc gtgccggtga cggtcgcgct gccggcgtaa
    21061 tcgaagccgg tcgaggagcg aaccttgtac gtgtcccagc cggggctgtt gatgcccgag
    21121 cgcaggccac ccgtcgcacc ggctctaccc gaatgcgtgc ggccagcacc gaggacaaga
    21181 ccgatcgagt acttcgcctg gcccggcatc gtgtagcagg gcagcgtgat cgacttgtcg
    21241 ccgaccttca ccgtgatcat ctggtcgttc ttcacgccga gtgcgaccga agtctcgggc
    21301 gacacgagcg cgtagttgtc ccaggtgatc ttcgtcagga agtctggcgt ctcccacagc
    21361 cac
//
LOCUS       DASUFX010000320        14749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_188420,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000320 DASUFX010000000
VERSION     DASUFX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14749
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188420"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFV99_12800"
     CDS             <1..697
                     /locus_tag="VFV99_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060237.1"
                     /translation="VELFACIEAVWQPIGTARTNDDGRFSFALTGTKRLQIGMRDLFV
                     SVTGDRTGAAFIGFVAPTGTKMVVSDVDGTLTESENSYPESLALGGDVPVQPGAPAAL
                     QMLKARNYAVVYITSRGDRFTQDTRDWFAAKGFPRGPMRLPPAIVTLPGADTVEFKSS
                     AIESLAAFDVEAGFGNRSSDVTAYMENGISADRIFIKLPEFTEELMSVLSMSSATGFT
                     SYDDLRANQLMAM"
     gene            complement(701..2209)
                     /locus_tag="VFV99_12805"
     CDS             complement(701..2209)
                     /locus_tag="VFV99_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060238.1"
                     /translation="MRGSIGQLLISQGAIDAGALSDALARQSGRHPVASELYVLGYAT
                     ERQLCAALSVQTGWPAIVLDESTIRLDVLEHVSIEWARIFSALVVHEDERTLVVAAAR
                     PEDAIVPARELGSSRGKAVELRIALDVTLARTIRVAFRKWKAGETYLIGHDASTTRKG
                     AYLSIVQPDSGEDEHRQAQRALAEDAARNIERSEELPSEDHVSVSTLTGDWTSNTVTT
                     STEDTNNPLLEEKTLVLTADLDRLNEKEPQYSGTKALVVDPDPPGRIQLVTELEKLGW
                     SCQAVPNGAQAVELLASTPFDLVFADVAAAEVDGLQLCRAIKRSRRLAKTRVVITTSV
                     VDSGQVPDDVLQRHSADGYIEKPIDTRRLHRMLRDLSPDDKADHEGVLADALGRYHNG
                     DIEGAIAKLRGALVSDPSSPKLHFMLANMLQRASRWAEAVDEYEAVVELQPAYFPALT
                     RLAYLYFRPGLHARAVETWRRALPVCEDPALRRNIELFMRKLVADMSKAEHA"
     gene            2441..2926
                     /locus_tag="VFV99_12810"
     CDS             2441..2926
                     /locus_tag="VFV99_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5060239.1"
                     /translation="MTDFKIGEKAVYPAHGVAEVIGIEKKEINTTVCSFYVLKVLATG
                     MQILVPKDKADQVGLRPVATSSEIEEVFDILREKDVHIDKQTWNRRYRGFMEKIKTGS
                     LFDVAEVYRDLYRLKSSKVLSFGERKMLDNAKQLIVKELAVAKKWAEDKVEQELEKAC
                     A"
     gene            2976..3485
                     /locus_tag="VFV99_12815"
     CDS             2976..3485
                     /locus_tag="VFV99_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5060240.1"
                     /translation="MQVHVAKADIWSLPVDAVVNPANSLGIMGGGIGGSLRRQGGDVI
                     QQAAMAAAPIAVGAALVTTAGQLPAKNVIHVPTMEEPGMMIGAENVRRAARAALIAAD
                     RNNFKTIAIPGIGTDLGNVPIDEAARAIVEEIRAHRRPNPETIYLVDTNDYMVAALEE
                     ALRNAQANL"
     gene            3628..5286
                     /locus_tag="VFV99_12820"
     CDS             3628..5286
                     /locus_tag="VFV99_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060241.1"
                     /translation="MLRGLVVTAVLSAATIAAAQPQQARPSPAASAFDASGWTLLGTQ
                     EVAGKRDRDTFNVGKQEGKFDKLAIVVTDSDIELKDVTVFFANGDKWSPGGVKHVFKE
                     GQRSRAIDLPGNDRTIAKVELLYANTPGGGRAKVAVYAKDTKAKPAGKRPDMTGWTLL
                     GGQQVGGRKDRDTFNVSKYEGKFDEIQLNVLDSDLELLDLTVIFANGEKWSPKVKVSF
                     KEGQRSRAIDLPGNDRVISKIELVYANTRGGGNAYVEVYGRDRRKGPNGNPGPLPPNL
                     PTKPVFDANGWTLLGTQTVNGGRDKDTIRVGQKKGGFDQLTMVVTDSDLDLQDFTIVF
                     TGGQKWSPKFKHVFKEGARTRVIDLPGKDRTITRIELLYANLPGGGKAKVELYGRDVG
                     RPAPPPFTPIKWENKGWTFLGKKTVDGWRDRDKLNVFAAKPFSEVMFVVTGSDVQLNN
                     IVITLGNNEKFEMPGSVVFKEGTRTSPVDIPGVLRKIKSVEFAYANLPGGGRASVEVW
                     ARAKVQPPTPPPPTPAIATPPPPKPATPPPPPPPPAGPTVRDHR"
     gene            complement(5526..6014)
                     /locus_tag="VFV99_12825"
     CDS             complement(5526..6014)
                     /locus_tag="VFV99_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060242.1"
                     /translation="MKIKALVTCLVLGSSSLALAAPSYTTPYAAPNVRDHRQLPPAPA
                     PVAQYQGQFGWQKPLARPVLLADNTRVNGWSNINVAQGTRAFTKLELKANSGKTDLDR
                     VMITFGNGQRQVVSLRGKLDGKINAGKSLTIDLDGNGRFIKSVMLIGSSGRRASIDVV
                     AV"
     gene            complement(6205..6612)
                     /locus_tag="VFV99_12830"
     CDS             complement(6205..6612)
                     /locus_tag="VFV99_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060243.1"
                     /translation="MRSLVALVLLAGCVPLSYTYTPSTNKPVSRKPDNCKFEVYTTPP
                     QEGYEELGVLDLYNGTAPNDPAKFKSAVAEQVCGNGGDGVIAIRDDKGAISKGQIIHF
                     AHFTEPVKPISDMPSTPSTPSTQEGDTENPNKK"
     gene            6765..7151
                     /locus_tag="VFV99_12835"
     CDS             6765..7151
                     /locus_tag="VFV99_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5060244.1"
                     /translation="MQLQAYMPAGLAIVLALGFCGLFTALAVLIGPKATNPEKLKPFE
                     CGSEPIGTPRGRYSVKFYQVAILFLVFDIEAAFMYPWSIMYNQLSHDPMTGAVSVFGF
                     VEMLVFVAILVVALAYVWRKKAIGWE"
     gene            7142..7681
                     /gene="nuoB"
                     /locus_tag="VFV99_12840"
     CDS             7142..7681
                     /gene="nuoB"
                     /locus_tag="VFV99_12840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX5060245.1"
                     /translation="MGVELSTTRLEDAAKWAKQSLLERGSNWGRKFSLFSYPFVTACC
                     GMEFMSVAGPKYDLARFGAEFPRFSPRQSDLLMVVGTITEKQGPALKRVFDQMAEPKW
                     VIAFGVCASTGGFYQNYHAMPGCDQVIPVDVYIPGCPPRPEQVLDAIILLMDRIQNGR
                     GHSQLRTKREELIAASTDE"
     gene            7694..8239
                     /locus_tag="VFV99_12845"
     CDS             7694..8239
                     /locus_tag="VFV99_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5060246.1"
                     /translation="MAKQILDALAAKFAHAVERTESNFGDEVAWIKRENLVEVAKWLR
                     DEQGFAFPVFCTCIDRLDWKPIGLPPSEHWNESKPRFEIAYQLRSLKNQRIRLKVAVT
                     EADPRVQSLAELWPAFNWQERETFDMYGIRFDGHPDLRRIYLYEEFVGYPLRKDYPKE
                     KRQPLVRRDDLILNPLKKEVR"
     gene            8239..9489
                     /gene="nuoD"
                     /locus_tag="VFV99_12850"
     CDS             8239..9489
                     /gene="nuoD"
                     /locus_tag="VFV99_12850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX5060247.1"
                     /translation="MATESLKQLVLGRKDVQDLSDTETDLPPDLMTVNMGPSHPAMHG
                     TVRIVLTVDGERIVKSDVQPGYLHRCFEKESEHATYTQVFPYTDRLNYVSPMINNVGW
                     AMAVEKIMGLTPQIPKRVEYIRVIVSEMSRLTDHLTCVGASAMELGAFTAFLYFLKAR
                     EWLYELLEVVSGARLTHSYVRVGGVAKDLPEHFHTELTTCLDKIAGVMVEVEVMLNNN
                     KIFRDRMAGVGAMTKEDAIATGCVGPIARACGVDYDVRRDHPYSVYPEVEFDVPLGTH
                     GDCYDRYLVRIEEMKQSIRILRQCLRQIPAGPTMVDDPRVALPPKRDTYNTIESMIRH
                     FKHIMDGIRVPPGEAYTFVEGGNGEVGFFIVADGTGRPYKAYCRSPSFVHLSTSSQLI
                     HNHLIADVVPIFGMINMIGGECDK"
     gene            9486..11051
                     /locus_tag="VFV99_12855"
     CDS             9486..11051
                     /locus_tag="VFV99_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5060248.1"
                     /translation="MSDTVTLTIDGQQVTVPKGTNVLEAARSLGNDISAFCYHPGLPI
                     VAQCRQCLVSIEKNPKLQPSCQQVCGDGMVVHTKDPQSTLARKQQLEFTLLNHPIDCP
                     ICDKAGECTLQKLYFDHDNENSRVDVPKVHKDKVVDVGPHIVLDQERCILCSRCIRTC
                     DEVAGQHQLEFANRGDHEVLTTAPGEQLDNPYSINTVDVCPVGALTAKDFRFTMRAWE
                     LEATPSICNGCGTGCNIEIHHKQGRAWRLVPRHNPDVNKYWMCDEGRFTYHALREQRL
                     ASALVDGLPSTWDRAVTNAAKRLGVFLKDASQIGVVLSAQHSNEDNFALAQLAKQWGV
                     KQVYLAGRPPVPARADGKLRVADVNPNTSGAKAIAEALGLGVVQAFQIDASAASTLRA
                     LVVLGDTFTGLTEGKLREIDLITISAFERGPVAHAKVALPAAAWAENAGTVTNVQGRV
                     QRMHAAFAPPGNAIPAWEAIVRLAHATGVKLAWTHAREVFKDMTAAVPAWKELTWARE
                     ARPLALRFAGSRG"
     gene            11051..12268
                     /locus_tag="VFV99_12860"
     CDS             11051..12268
                     /locus_tag="VFV99_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX5060249.1"
                     /translation="MTASLAAALPSWVPSGWAVLDFPATKWAILILLLVMPLASLLTW
                     AERRQSAMMQDRLGPNRANIGPIKLKGILHFVADALKMIFKEDFIPANVHRGLFALAP
                     VLALAPVLIAFCIIPFGPTIFPHVFTKVLDVNELATNPAAPQFADSIRLQIFQADWGL
                     IFYFAVLTLANYGGTIAGWASYNKWALLGGLRSSSQMMSYEVAMGMSLMGAFLVVGSL
                     EPGYIVAHGASADMSPAHPLNWLWLWQPVALVLFFTAAIAETKRAPFDIPEGEPEIIG
                     YFVEYSGIRWGMFFLAEFIEIVFISAVIATVFFGGYHVPFLEPYGFSIQGFGSPTGAW
                     TLLLPHGVVVGLQILAFGAKVVLLCWFQLMIRWTLPRFRADQLMNLGWKLLLPLSLAN
                     IMVTALIKLIFMK"
     gene            12277..12975
                     /locus_tag="VFV99_12865"
     CDS             12277..12975
                     /locus_tag="VFV99_12865"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX5060250.1"
                     /translation="MSAAHHDTPEVSDGPKVLAEKTSSAGKKVRTLQVVRPEPQDASF
                     LAASAKGFGVTLKHFARNFFLPLRKAAARAKAGAPVDEKWKNEIETVQYPEEKVKYPE
                     RFRGLHRLMLRDDGAVRCVACMCCPTVCPAHCITIIPAETDNKGIEKKPAVFEIDELR
                     CVVCGLCVEACPCDAIRMDTGEHAKPTEERADGIETLESMLARGIRSTAKQGGEGPGW
                     RETAPHTPDTEVNR"
     gene            13212..13384
                     /gene="ssrS"
                     /locus_tag="VFV99_12870"
     ncRNA           13212..13384
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFV99_12870"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13453..>14749
                     /locus_tag="VFV99_12875"
     CDS             13453..>14749
                     /locus_tag="VFV99_12875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060251.1"
                     /translation="MDRDTNVTAVTEVQAGRGPARDTVLLGKYRVESTLGFGGMGLVI
                     KAHNLALDEHVAIKLLREDVQVDNENATRFVREAKNAVKLKSEHVARIRDVGTFDDGR
                     PYMVMELLEGLDLGKILLDQGVVERDRAVDLVLQACDAIAEAHSLGIVHRDIKPTNLF
                     VTKRRDGTDLLKVLDFGISKATMGTDMNLTQTQSMLGTPAYMSPEQMRSARTVDPRSD
                     IWSLGCVLYELVEGHPPFEAENFAELCVMVSIEPFTPIKNAPELAAVIDKVLAKPIEQ
                     RFQSIAELAHALEPFSSDPERARRQVARVFRVLGRSHPEGRDSTPTPMPMAAARGSSS
                     RISQPRISSSARPQTPIPNVRDRSGLWKMLLALGALAVIGAGSWMLLMGQDHDPDSAL
                     EPAAGSSADAVEPAPAGSTTEMTVERADAAVEATEAVSPA"
BASE COUNT         2672 a         4790 c         4734 g         2553 t
ORIGIN      
        1 cgtcgagctg ttcgcgtgca tcgaagcggt atggcaaccg atcggcaccg cgcgcaccaa
       61 tgacgatggt cggttctcgt tcgcgctcac cggcaccaag cgcctgcaga tcggcatgcg
      121 cgatctgttc gtctcggtga cgggcgatcg caccggcgcc gcgttcatcg gcttcgtcgc
      181 gccgaccggt acgaagatgg tcgtgtcgga tgtcgatggc acgctcaccg aatcggagaa
      241 ctcgtatccc gagtcgctcg cgcttggcgg cgacgtccct gtacaacccg gcgcacctgc
      301 ggcactgcaa atgctgaagg cgcgcaacta cgccgtcgtt tacatcacgt cgcgcggcga
      361 tcgcttcacg caagacactc gcgactggtt cgccgcgaag ggcttcccgc gcggtccgat
      421 gcggttgccg cccgcgatcg tcacgctgcc cggcgcggac accgtcgagt tcaagagcag
      481 cgcgatcgaa tcgctcgccg cgttcgatgt cgaggctggc ttcggcaatc gctcgagtga
      541 tgtcaccgcg tacatggaaa acggcatctc cgccgaccgc atcttcatca agctgcccga
      601 gttcaccgag gagctcatgt ctgtgctctc gatgagctcg gcgaccggct tcacgtcgta
      661 cgacgatctg cgcgcgaacc agctgatggc gatgtgaagc tcaggcgtgc tccgccttcg
      721 acatgtcggc gacgagcttg cgcatgaaga gctcgatgtt gcgccgcagt gccgggtcct
      781 cgcagaccgg caacgcacgc cgccaggtct cgaccgcgcg cgcgtgcagg cctgggcgaa
      841 agtagagata cgcgagccgg gtcagcgccg ggaagtacgc gggctggagc tcgacgaccg
      901 cctcgtactc gtcgactgcc tccgcccacc gcgacgcacg ctgcagcatg ttcgcgagca
      961 tgaagtgcag cttcgggctc gacggatccg agacgagcgc accgcgcagc ttcgcgatcg
     1021 cgccctcgat gtcaccgttg tgatagcggc cgagcgcgtc ggcgagcacg ccctcgtggt
     1081 ctgccttgtc gtcgggcgac agatcacgga gcatgcgatg caagcggcgc gtgtcgatcg
     1141 gcttctcgat gtagccgtcg gcggagtggc gctgcaacac gtcgtcggga acctggcccg
     1201 aatcgacgac cgacgtcgtg atcacgacgc gtgtcttcgc gagccggcgg ctgcgcttga
     1261 tcgcgcgaca cagttggagg ccatcgacct ccgccgcagc gacgtccgca aacacgaggt
     1321 cgaacggcgt gctcgcgagc agctcgaccg cctgcgcgcc gttcggcacg gcctgacagc
     1381 tccagcccag cttctcgagc tcggtcacga gctggatgcg tcccggcgga tctggatcga
     1441 cgacgagtgc tttcgtgccg ctgtactgcg gctccttctc gttgagtcga tcgaggtccg
     1501 cagtgagtac gagcgtcttt tcttcgagca gcggattgtt cgtgtcctcg gtcgatgtcg
     1561 tgaccgtgtt cgacgtccag tcgccggtca gcgtcgacac cgacacgtga tcctccgagg
     1621 gcagctcctc gctacgctcg atgttgcgcg ctgcgtcctc ggcgagcgcg cgctgtgctt
     1681 ggcgatgctc gtcctcgccg ctgtcgggct gcacgatcga caggtacgcg cctttgcgcg
     1741 tcgtgctcgc gtcgtgaccg atcaggtacg tctcgcccgc cttccatttg cggaacgcga
     1801 cgcgaatcgt gcgtgcgagc gtgacgtcga gtgcgatgcg gagctcgacg gccttgccgc
     1861 gtgagctgcc gagctcgcgc gccggcacga tcgcgtcctc gggtcgggcg gcagcgacga
     1921 ccagcgtgcg ctcgtcttcg tggacgacca gcgcgctgaa aattcgcgcc cactcgatcg
     1981 acacgtgctc gagcacgtcg agccggatcg tcgactcgtc cagcacgatc gcgggccagc
     2041 cggtctgcac cgagagcgcg gcacacagct ggcgctcggt cgcgtacccg agcacataga
     2101 gctcggacgc caccgggtgc cgaccactct ggcgagcaag cgcgtcagag agcgcacctg
     2161 cgtcaatcgc gccttgcgag atcaagagtt ggccgatcga gccccgcact cgcgcacagg
     2221 gtaacaccgc caagtatgca aaatctcgac tgacgtacgg ctagccgatc cgccggatgt
     2281 cgatttggct ggagattcgg cggatggctg atatcctccg cgtcccgttg ggacccccta
     2341 tgtcccgcgc gtgagacact ctggcgcacc tcgattagct aacccagcgt taatcttcga
     2401 gacaaccgga ggcaccagca ttgcttaagc ggagtactcc atgaccgact tcaagattgg
     2461 cgagaaggcc gtctaccccg cccacggggt ggccgaagta atcggaatcg agaagaagga
     2521 gatcaacacg acggtgtgct ccttctacgt tctgaaggtt ctcgccaccg gtatgcagat
     2581 cctcgtgccg aaggacaagg cggaccaagt gggcctgcgc ccggtcgcca cctcgtcgga
     2641 aatcgaggaa gtgttcgaca tcctgcgcga gaaggatgtc cacatcgaca agcagacctg
     2701 gaatcgccgc tatcgcggtt tcatggagaa gatcaagact ggctcgctct tcgacgtcgc
     2761 cgaggtctat cgcgacctgt accggctcaa gagcagcaag gtcctctcgt tcggcgagcg
     2821 caagatgctc gacaacgcca agcagctgat cgtgaaggag ctcgcggtcg cgaagaagtg
     2881 ggccgaagac aaggtcgagc aggagctcga gaaggcctgc gcctgaatcg cctagttgaa
     2941 tcgtaggctg ggcgcgtgct aacggtgcgg cgtccatgca ggttcacgta gcgaaggcag
     3001 atatctggtc gttgcccgtc gacgcggtcg tcaatccggc caactcgctg ggcatcatgg
     3061 gcggtggcat cggtgggtcg ctgcgtcgcc aaggcggtga cgtgatccag caggccgcga
     3121 tggcggctgc ccccatcgct gtcggcgctg cgctcgtgac aaccgcaggc cagctacccg
     3181 cgaagaacgt gatccacgtt ccgacgatgg aagagcccgg catgatgatc ggcgccgaga
     3241 acgtgcggcg cgcggcgcgt gctgcgctca tcgctgccga tcgcaacaac ttcaaaacga
     3301 tcgcgatccc cggcatcggc accgatctcg gcaacgtgcc gatcgatgaa gccgcgcgcg
     3361 cgatcgtcga agagattcgt gcgcaccgcc gcccgaatcc cgagacgatc tatctcgtcg
     3421 acacgaacga ctacatggtc gctgcgctcg aagaagccct tcgtaacgcg caagcaaacc
     3481 tgtaacgcgc gcaacgagga ttcgtcgcgc ggggcaaatc gcctcgcgtg aaatctcaca
     3541 gatgatgaat tcgctgcgaa ttcactccgt ctcgcacgag gacgcgacgc gctcgcgtta
     3601 gcgtcgttgg actcatacgg aggacacatg ttgcgaggct tggtagtcac cgcagtactc
     3661 tccgccgcga cgatcgcggc agcacagccg cagcaagcgc ggccgtcgcc agctgcgtcg
     3721 gcattcgacg cgagcgggtg gacgttgctc ggtacgcaag aggtcgcggg taagcgcgac
     3781 cgcgacacgt tcaacgtcgg caagcaggaa ggcaagttcg acaagctcgc gatcgtcgtc
     3841 accgactcgg acatcgagct caaggacgtg acggtgttct tcgcgaacgg tgacaagtgg
     3901 tcgccgggcg gcgtcaagca cgtgttcaag gaaggtcagc gcagccgcgc gatcgatctt
     3961 cccggtaacg accgcacgat cgcgaaggtc gagctcctct acgcgaacac gcccggcggc
     4021 ggccgcgcga aggtcgcggt ctacgcgaag gacaccaagg caaagccggc gggcaagaga
     4081 cccgacatga ccggctggac gctcctcggc ggtcagcagg tcggcggtcg caaggatcgc
     4141 gacacgttca acgtcagcaa gtacgagggc aagttcgacg agatccagct caacgttctc
     4201 gactctgacc tcgagttgct cgacctgacc gtcatcttcg cgaacggcga gaagtggtcg
     4261 ccgaaggtca aggtgtcgtt caaagagggt cagcgcagcc gcgcgatcga cctgccgggc
     4321 aacgaccgcg tgatctcgaa gatcgagctc gtctacgcga acacgcgcgg aggcggcaac
     4381 gcctacgtcg aggtctacgg ccgcgatcgc cgcaagggac cgaacggcaa cccgggtccg
     4441 ctaccgccga acctgccgac gaagccggtc ttcgatgcga acggctggac gctgctcggc
     4501 acgcagaccg tcaacggcgg ccgcgataag gacacgattc gggtcggcca aaagaagggc
     4561 ggcttcgatc agctgacgat ggtcgtcacc gactcggacc tcgacctcca ggacttcacg
     4621 atcgtcttca ccggcggcca gaagtggtcg ccgaagttca agcacgtctt caaggaaggc
     4681 gcgcgcacgc gcgtcatcga tctccccggc aaggaccgca cgatcacgcg catcgagctg
     4741 ctctacgcga acctgcccgg tggcggcaaa gcgaaggtcg agctctacgg ccgcgacgtc
     4801 gggcgccctg cgccgccgcc gttcacgccg atcaagtggg agaacaaggg ctggacgttc
     4861 ctcggcaaga agaccgtcga cggctggcgc gatcgcgaca agctcaacgt gttcgccgcc
     4921 aagccgttct ccgaggtgat gttcgtcgtc accggcagtg acgtgcagct gaacaacatc
     4981 gtcatcacgc tcggcaacaa cgagaagttc gagatgccgg gcagcgtcgt cttcaaagaa
     5041 ggcacgcgca cctcgccggt cgacatcccg ggcgtgctcc gcaagatcaa gagcgtcgag
     5101 ttcgcatacg cgaacctgcc gggtgggggg cgcgcgagcg tcgaagtgtg ggcgcgcgcg
     5161 aaggtgcaac cgccaacgcc gccgccgcct acgccagcga ttgcaacgcc gccgccgccg
     5221 aagccggcta cgccgccgcc gcctccgccg ccgccggcag gcccgaccgt ccgcgatcat
     5281 cgctaactag cgaggcgtga tgaaacgagg cacgtgccac gtggtacgtg ccggtttcgt
     5341 tttcggataa accgccaaga cgccgcagac gccaagtcgg aaagcgatct ttagaccgag
     5401 ccattggcga caaacggtcg cgcttggcac gtagcgtgtc cgagcacagc gcgatcgagc
     5461 tcgactctga tcttggcgtc cgcggcgcct tgtcggttgg ctccaggcgc gttggccgtg
     5521 tcgcattaga ccgcgacgac gtcgatcgac gcgcggcgac cgctcgagcc gatcagcatg
     5581 acgctcttga tgaagcgacc gttgccgtcg agatcgatcg tgagcgactt gcccgcattg
     5641 atcttgccgt cgagcttgcc gcgcaacgac acgacctggc gctggccgtt accgaacgtg
     5701 atcatcacgc gatcgagatc ggtcttgccg ctgttcgcct tgagctcgag cttggtgaac
     5761 gcgcgcgtac cctgcgcgac gttgatgttc gaccagccgt tcacgcgcgt gttgtcggcg
     5821 agcaggaccg ggcgcgcgag cggcttctgc cagccgaact ggccctggta ctgcgcgaca
     5881 ggcgcaggcg cgggcggcag ctgacgatgg tcgcgaacgt tgggcgctgc gtacggcgtg
     5941 gtgtacgagg gcgccgcgag cgcgagcgac gacgaaccga gaacgagaca ggtaacgaga
     6001 gctttgatct tcatgacagg gactccttgg ccgagcacca aaccagtgct cgccaggtcc
     6061 ctagtgcagc ggccgcgcca gcgctaagtg cgcgatggga caggcactac tcgggagcgc
     6121 cgctgcgaag ctcgcagacg acctgcggag tctgcagatc cgcggacatg gggtctgacc
     6181 ggtctgaccc ttcttgcggg gcggctactt cttgttcggg ttctcggtgt cgccctcctg
     6241 cgtggagggg gtcgagggtg tcgagggcat gtccgagatc ggcttcaccg gctccgtgaa
     6301 gtgcgcgaag tgaatgatct ggcccttcga gatcgcgccc ttgtcgtcgc ggatcgcgat
     6361 cacgccgtca ccgccgttgc cgcagacctg ctccgcaacc gcgctcttga acttcgcggg
     6421 gtcgttgggc gccgtgccgt tgtagaggtc gaggacaccg agctcctcgt agccctcttg
     6481 cggcggggtc gtgtagacct cgaacttgca gttgtcgggc ttcctgctga ccggtttgtt
     6541 tgtagacggc gtgtacgtgt acgagagcgg gacacaaccg gcgagcagca cgagcgcgac
     6601 gagtgaacgc atagcggtca tggtgcccgc gccttgcgtg cgggtccagc attcggcggc
     6661 gctggatcgg ccatcagcgt cgggccgaac cccgcgatcg gcctcgaaaa aagcccgccc
     6721 ggccgcgttg acccgcctat ctagcgccat atactgcgct cgctttgcag ctccaggcgt
     6781 acatgccagc aggactggcg atcgtgcttg cccttggctt ctgcggcctg ttcacggcgc
     6841 tcgccgtgct catcggcccg aaagccacta accccgagaa gctgaagccg ttcgaatgcg
     6901 gtagcgagcc gatcggaact ccgcgtggcc gctactcggt caagttctac caagtcgcca
     6961 tccttttcct cgtcttcgac atcgaagctg cgttcatgta cccgtggtcg atcatgtaca
     7021 accagctctc gcatgacccg atgacgggcg cggtcagcgt gttcggcttc gtcgagatgt
     7081 tggtgttcgt cgcgatcctc gtggtcgcgc tcgcctacgt ctggagaaag aaggcgatcg
     7141 gatgggagta gagctctcca ccacgcgact cgaagacgcc gccaagtggg cgaagcagtc
     7201 gctcctcgaa cgcggctcca actggggacg taagttctcg ctgttctcgt acccgttcgt
     7261 caccgcgtgc tgcggcatgg agttcatgtc cgtcgcggga ccgaagtacg acctcgcacg
     7321 cttcggcgcc gagttcccgc ggttctcgcc gcgccagagc gacctgctca tggtcgtcgg
     7381 cacgatcacc gagaagcagg gccccgctct caagcgcgtg ttcgaccaga tggccgaacc
     7441 gaagtgggtc atcgcgttcg gcgtctgcgc gtcgacgggc ggcttctacc agaactatca
     7501 cgcgatgccc ggctgcgatc aggtcatccc ggtcgacgtc tacatcccgg gttgtccgcc
     7561 gcgtcccgag caagtgctcg acgcgatcat cctgctcatg gatcgcatcc agaacgggcg
     7621 cggtcacagc caactccgta cgaagcgcga agagctcatc gccgcttcga cagacgaata
     7681 gcgaggagtc gtcatggcga agcaaatcct cgacgcgctg gcggcgaagt tcgcacatgc
     7741 cgtcgagcgc accgaatcga actttggcga cgaggtcgcc tggatcaagc gcgagaacct
     7801 cgtcgaagtg gcgaagtggc tgcgcgacga gcagggcttt gcgttcccgg tgttctgcac
     7861 ctgcatcgat cgcctcgact ggaagccgat cggcctgccg ccgagcgagc actggaatga
     7921 atcgaagccc cgcttcgaga tcgcctacca gctgcgctcg ctgaagaacc aacgcattcg
     7981 cctcaaggtc gcggtcaccg aagccgatcc gcgcgtgcag tcgctcgccg agctgtggcc
     8041 ggcgttcaac tggcaggagc gcgagacctt cgacatgtac gggatccgct tcgacggcca
     8101 ccccgacctc cgccggatct acctgtacga agagttcgtt ggctatccgc tgcgcaagga
     8161 ctatccgaag gagaagcggc agccgctcgt gcgccgcgac gacctcatcc tcaatccgct
     8221 caagaaagag gtgcggtgat ggcgaccgag tccctcaagc agctcgtgct cgggcgcaag
     8281 gacgtccagg atctctcgga caccgagacg gatctgccac cggatctcat gaccgtgaac
     8341 atgggcccgt cgcatcccgc gatgcacgga accgtgcgca tcgtgctcac cgtcgatggc
     8401 gagcgcatcg tcaagagcga cgtccagccc ggctatctgc atcgctgctt cgagaaggag
     8461 agcgagcacg ccacgtacac gcaggtgttc ccgtacacgg atcgcctgaa ctacgtgtcg
     8521 ccgatgatca acaacgtcgg ctgggcgatg gcggtcgaga agatcatggg gctcacgcct
     8581 cagatcccga agcgcgtgga gtacatccgc gtgatcgtca gcgagatgtc gcgcctgacc
     8641 gatcacctga cgtgcgtcgg cgcgagcgcg atggagctcg gcgcgttcac cgcgttcctc
     8701 tacttcctca aagcccgcga gtggctctac gagctgctcg aggtcgtcag cggcgcgcga
     8761 ctcacgcaca gctacgtgcg cgtcggcggc gtcgcgaagg atctccccga gcacttccac
     8821 accgagctca ccacctgcct cgacaagatc gccggcgtga tggtcgaggt cgaggtgatg
     8881 ctcaacaaca acaagatctt ccgcgatcgc atggccggcg tcggcgccat gacgaaggaa
     8941 gatgcgatcg cgaccggctg cgtcggtccg atcgcgcgcg cgtgtggcgt cgactacgac
     9001 gtccgccgcg accatccgta ctcggtgtat cccgaggtcg agttcgacgt gccgctcggc
     9061 acacacggcg actgctacga ccgctacctc gtgcgcatcg aggagatgaa gcagtcgatc
     9121 cgcatcctgc gtcagtgtct gcgccagatt cccgccggcc cgacgatggt cgacgatccg
     9181 cgcgttgcac tgccgccgaa gcgcgacacg tataacacga tcgagtcgat gatccgtcac
     9241 ttcaagcaca tcatggacgg catccgcgtg ccgcccggtg aggcgtacac gttcgtcgag
     9301 ggtggcaacg gcgaggtcgg cttcttcatc gtcgccgatg gcaccggccg tccctacaag
     9361 gcgtactgcc gttcgccgtc gttcgtgcac ctgtcgacgt cgagccagct gatccacaac
     9421 cacctcatcg ccgacgtcgt gccgatcttc ggcatgatca acatgatcgg tggagagtgc
     9481 gataaatgag tgacacggta actctcacta tcgacggcca gcaggtcacg gtcccgaagg
     9541 gcacgaacgt gctcgaggcc gcgcgctcgc tcggcaacga catcagcgcg ttctgctacc
     9601 accccggcct tccgatcgtc gcgcagtgcc gccagtgcct ggtctcgatc gagaagaacc
     9661 cgaagctgca gccgagctgc cagcaggtct gtggcgacgg catggtcgtc cacacgaagg
     9721 acccgcagtc gacgctcgcg cgcaagcagc agctcgagtt cacgctgctc aaccacccga
     9781 tcgattgccc gatctgcgac aaggcgggcg agtgcacgct gcagaagctg tacttcgatc
     9841 acgacaacga gaactcgcgc gtcgacgtcc cgaaggtcca caaggacaaa gtcgtcgacg
     9901 tcggcccaca catcgtcctc gatcaagaac gctgcatcct gtgctcgcgc tgcatccgca
     9961 cctgtgacga ggtcgccggc cagcatcagc tcgagttcgc caaccgcggc gatcacgagg
    10021 tgctgacgac ggcgccgggc gaacagctcg acaacccgta ctcgatcaac accgtcgatg
    10081 tctgcccggt cggcgcgctg accgcgaagg acttccggtt cacgatgcgc gcgtgggagc
    10141 tcgaagcgac gccgagcatc tgcaacggct gcggcaccgg ctgcaacatc gagatccatc
    10201 acaagcaggg ccgcgcgtgg cgcctcgttc cgcgccataa cccggacgtc aacaagtact
    10261 ggatgtgcga cgagggccgg ttcacgtacc acgcgctgcg cgagcaacgc ctcgcgagtg
    10321 cgctcgtcga cggcctgccg agcacctggg atcgcgcggt gacgaacgcc gcgaagcgac
    10381 tcggcgtgtt cctcaaggat gcgtcgcaaa tcggcgtcgt cctctcggcg cagcactcga
    10441 acgaagacaa cttcgcgctc gcgcagctcg caaagcagtg gggcgtcaag caggtctacc
    10501 tcgccggtcg cccgccggtg cccgcgcgtg ccgatggcaa gctgcgcgtc gccgacgtca
    10561 acccgaacac gagcggcgcg aaggcgatcg cggaagcgct tggcctcggc gtcgtgcaag
    10621 cgttccagat cgacgccagc gcggcctcga cgctgcgtgc gctcgtcgtg ctgggcgata
    10681 cgttcaccgg cctgactgaa ggcaagctgc gcgagatcga cctgatcacg atttccgcat
    10741 tcgagcgcgg tccggtcgcc catgcgaagg tcgcgttgcc cgcggccgcg tgggcggaga
    10801 acgccggcac ggtgacgaac gtgcaaggtc gcgtgcagcg tatgcacgcg gcgttcgcgc
    10861 ctcccggcaa tgcgattccg gcgtgggaag cgatcgtgcg actcgcccac gcgacgggcg
    10921 tgaagcttgc gtggacacat gcacgcgagg tgttcaaaga catgaccgcg gcagttcctg
    10981 cttggaagga gctgacgtgg gcacgtgagg cgcggccact tgcgctgcgc ttcgcgggga
    11041 gccgaggcta atgactgcat cactcgcagc cgcgttgccg agctgggtac ccagtgggtg
    11101 ggccgtgctc gactttcccg cgaccaagtg ggccatcctg atcctgctcc tcgtgatgcc
    11161 gctcgcgtcg ctgctcacgt gggccgagcg tcgtcagagc gcgatgatgc aggaccgcct
    11221 cggtccgaac cgcgcgaaca tcggtccgat caagctgaag ggcatccttc acttcgtcgc
    11281 cgacgcgctg aagatgatct tcaaggaaga cttcatcccg gcgaacgtcc acaggggtct
    11341 gttcgcgctg gcaccggtcc tcgcgctcgc gccggtgttg atcgcgttct gcatcatccc
    11401 gtttgggccg acgatcttcc cgcacgtctt cacgaaggtg ctcgacgtca acgagctcgc
    11461 gacgaacccg gccgcgccgc agttcgccga ctcgatccgc ctgcaaatct tccaggccga
    11521 ctggggcctc atcttctact tcgcggtgct cacgctcgcg aactacggcg gcacgatcgc
    11581 cggctgggcg agctacaaca agtgggcgct gctcggcggc ctccgcagct cgtcgcagat
    11641 gatgtcgtac gaggtcgcga tgggcatgtc gctcatgggc gcgttcctcg tcgtcggcag
    11701 cctcgagcct ggttacatcg tcgcgcacgg cgcgtcggcc gatatgtcgc cggcgcatcc
    11761 actcaactgg ctatggctct ggcagccggt cgcactggtg ttgttcttca ccgcggcgat
    11821 cgcggagacg aagcgcgcac cgttcgatat ccccgaaggc gaacccgaga tcatcggcta
    11881 cttcgtcgag tactcgggca ttcgctgggg catgttcttc ctcgccgagt tcatcgagat
    11941 cgtcttcatc tcggcggtga tcgcgaccgt gttcttcggc ggctaccacg tgccgttcct
    12001 cgagccgtat ggcttcagca tccagggctt cggaagcccg accggtgcgt ggacgctgct
    12061 gttgccgcac ggtgtggtgg tcggtctgca gatcctcgcg ttcggcgcga aggtcgtcct
    12121 gctgtgctgg ttccagctga tgattcgttg gacgctgccg cgcttccgcg ccgatcagct
    12181 gatgaacctc ggctggaagc tgctcctccc gctgtcgctc gccaacatca tggtgacggc
    12241 gctgatcaag ctcatcttca tgaagtagag gcgaacatgt cagccgctca tcacgacaca
    12301 cccgaagtat ctgacggccc gaaggtcctc gcagagaaga cctcgtcggc gggcaagaag
    12361 gtccgcacgc tgcaagtcgt acggcccgag ccgcaagacg cgagcttcct ggcggcgagc
    12421 gcgaagggct tcggcgtcac gctcaagcac ttcgctcgca acttcttctt gccgctccgc
    12481 aaggccgccg cacgcgcgaa ggccggcgca ccggtggacg agaaatggaa gaacgagatc
    12541 gagaccgtgc agtaccccga ggagaaggtg aagtacccgg agcgcttccg tggtcttcac
    12601 cgcctcatgc tgcgcgacga cggcgcggtt cgctgcgtcg cgtgcatgtg ctgcccgacc
    12661 gtgtgtcccg cgcactgcat cacgatcatt ccggccgaga ccgacaacaa aggcatcgag
    12721 aagaagccgg cggtgttcga gatcgacgag ctgcgctgcg tggtgtgcgg cctgtgtgtc
    12781 gaagcgtgcc cgtgcgatgc gattcgtatg gacaccggcg agcacgcaaa gccgaccgaa
    12841 gagcgcgcgg atggaatcga aacgctcgag tcgatgctcg cgcgcggtat tcgctccact
    12901 gcgaagcagg gtggcgaagg accaggctgg cgcgagaccg cgccgcacac gcccgacacc
    12961 gaagtaaatc gctagcgctt cacggacgcg ctcacgcatt cttcgtcgat gtcgatcgct
    13021 tgacagcgat ccaattcacc gcgtgaacac gtcgttccga agcgccgatt catcgcgatg
    13081 tcaacgcgcg tttttgcgtc gctcggcaaa tttgcgatct gtgacgagat tttttttctt
    13141 tgcggtgaag tgatccgatc cgcatttttt caattgacgg attggatcgc gagccgtagt
    13201 ttcgaaaacg agcccggctg ttcgatgcga tacccggggc caatctgtct cctacatttc
    13261 tatccaggga tctgcctctg ttccgggagt gcacgcccgc tcgtcgggaa gcccaaagga
    13321 ttatgcggag cccacctacg taaatcgtag ggggtagacg gctctacggt cgaacggtcg
    13381 tgctcgtttt ggggtgcagc ggatgacagc cgctgcaccc cactgtttat ttcgggctac
    13441 ccttgaagcg gtttggaccg ggacacgaac gtcacggcag tgacggaggt gcaggccggg
    13501 cgggggcccg cacgcgatac cgttttactc ggtaagtatc gcgtcgagtc gacccttggc
    13561 ttcggcggca tggggctcgt catcaaggcg cacaaccttg cgcttgacga gcacgtcgcc
    13621 atcaagctgc tgcgcgaaga cgtccaggtc gataacgaga acgccacgcg ttttgtccgt
    13681 gaggcaaaga acgctgtgaa gctcaagagt gagcacgtcg cccgcatccg cgacgtcggc
    13741 acgttcgacg acggccggcc gtacatggtg atggagctgc tcgagggcct cgacctcggc
    13801 aagatcctgc tcgatcaggg agtggtcgaa cgcgaccgcg cggtcgacct cgtgctgcaa
    13861 gcgtgcgacg cgatcgccga agcgcactcg ctcggcatcg tgcatcgcga catcaagcca
    13921 acgaacctgt tcgtcaccaa gcggcgcgac ggcaccgatc tgctcaaggt gctcgacttc
    13981 gggatctcga aggcgacgat gggcaccgac atgaacctga cgcagacgca gtcgatgctc
    14041 ggcacgcccg cgtacatgtc tccggagcag atgcgatcgg cacgcaccgt cgatccgcgc
    14101 agcgacatct ggtcgctcgg ctgcgtgctc tacgagctcg tcgaaggcca tccaccgttc
    14161 gaggcggaga acttcgccga gctgtgcgtg atggtgtcga tcgagccatt cacgccgatc
    14221 aagaacgctc ccgagctcgc agcggtgatc gacaaggtgc tcgcgaaacc gatcgagcaa
    14281 cgatttcaga gcatcgccga gctcgcgcac gcgctcgagc cattctcgag cgatccggaa
    14341 cgagcacgcc gccaggtcgc gcgcgtgttc cgcgtgctcg gtcgttcgca cccggaaggt
    14401 cgcgactcga cgccgacgcc gatgccgatg gcggcagcgc gcggcagtag ctcgcggatc
    14461 agccagccga ggatttcgtc gtcggcgcgg ccgcaaacgc cgataccgaa cgtgcgcgat
    14521 cgcagcgggc tatggaagat gctcctcgcg ctcggtgcgt tggccgtgat cggtgccggg
    14581 agctggatgc tcctgatggg ccaggatcac gatccagatt ccgcattgga gccagcggct
    14641 ggtagtagtg ctgacgccgt cgagcccgcg ccggctggct cgacgaccga gatgaccgtc
    14701 gagcgcgccg acgcagccgt cgaggccacc gaagccgtat cgcccgccg
//
LOCUS       DASUFX010000321        15863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1854857, whole genome shotgun sequence.
ACCESSION   DASUFX010000321 DASUFX010000000
VERSION     DASUFX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15863
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854857"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFV99_12880"
     CDS             <1..209
                     /locus_tag="VFV99_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060252.1"
                     /translation="IQQKALTLFKTGYTKAIQMQVYDEYTAKIREALGRLDADHFPPE
                     KEGRSRERSADRPLNPDLMTEVAR"
     gene            206..1330
                     /locus_tag="VFV99_12885"
     CDS             206..1330
                     /locus_tag="VFV99_12885"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060253.1"
                     /translation="MKKLLVIVLVALTACGGSTKRTVRGGRTVEAKREKIKPAAMKEY
                     EAAMRALRLGGPEANETARARLRSAVKTDSTLWEGWYNLGVIAWREGDDDEAIDDYGK
                     ALAQNRDHTPTLMARAEAYRRAGRKKEARGDYEAALKGMEEDDPNRRDAAARLASLLR
                     DGGDYEDAVEVLRETVRVSGTNARIYTELGQIYIAQKRLELAQLVLAKALELDAKDPS
                     VYNALALLAQRQGKSQEAFERYDQAVSLDAKYIDARFNKATVLLDAGDYLRAKTELTA
                     IVENKQDDYAAWVSLGVAHRGLAEFPDARKAYERVIKEAPKRSSARADAMFNFAILKM
                     DFLNDQAGAKTELERYLQEAPTSHAKHQEAETLCKQLKCR"
     gene            1345..1653
                     /locus_tag="VFV99_12890"
     CDS             1345..1653
                     /locus_tag="VFV99_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060254.1"
                     /translation="MRGLLTTLFVCALVAPAAAQPKKGDKGPPPQEAKQKAPEKGEKK
                     EKDKVKNFDFNALDLNGRMRTPQLLYFLERANEELERASLEKRSFIPHMVRSVEEEQL
                     "
     gene            1650..3254
                     /locus_tag="VFV99_12895"
     CDS             1650..3254
                     /locus_tag="VFV99_12895"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5060255.1"
                     /translation="MSTVALRTALIWQDEVMDDVVLQKPTKITVGSAGGSTFVVPNVG
                     LPKNFAIVRPGNRGYLLTLGERMRGTICIDGEQRDVAEYVKKNVERGGFAATPISGKD
                     WGVIDLDESGDYKVFFQFVPLDDATAQPFFTKQVITAGIAGYLLSIVTLAIFFYLRGV
                     STDFTLFNSALLAEFAEAGFRGWCIASAALGVSAIGWWLVQQEGDQQASFAFSVLLHA
                     ALLFMTYQLYDGSNPFVWPGPRSLTGSYLVTRLEPEPEPKPVPTVATVGQKKDTGAAA
                     PKSPEKPKNTATKNDEGAAGGKGETERARDPNAKDVPPAPPKVALFEDKNRKVLDNII
                     DRNLATSLDKFTGIKGDEVKRGSLGFGEGTGTGVGAGVGTGTTRGSNKNGSGGGGNAE
                     GDFVSNKGKIDTGKDRPGGGNCAKPPCGTAPKEVKVVLQDAEGDFGGLTAEEINRVVK
                     ARAGIFRACYQGELNRNPGLGGKLVVAFQIGGDGVVKSAKIGGGSSMRNDNVEGCVKS
                     NIMRLKFPAKGGIANVNYPFLFQPGG"
     gene            3257..4084
                     /locus_tag="VFV99_12900"
     CDS             3257..4084
                     /locus_tag="VFV99_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060256.1"
                     /translation="MRKLIFMVLITGCTNEPQYVSCAPMGAAAMDLCKLEGGMDDGMG
                     GTLDEVKGSVHIPLRREEDWKQSDQDKRTELMANAPAGVDVPIYRPEHYDISVEWTVK
                     NLTDQQSQFRVDLNGANEEFTYDPSMIILDPTDDEAPPTPPLAGNIPIDIGPNAEISG
                     TFREDQLVEAAIDLDQVTRGNVNPFRAVLTTSRHDDQFQPVTPYDYVNMTGGEPTGPA
                     VPSYAWRQIVRVDIVFKPQAHMTIDYTVRVREHVDVIPDEGLNDPDINVLDPAPYVP"
     gene            4110..4667
                     /locus_tag="VFV99_12905"
     CDS             4110..4667
                     /locus_tag="VFV99_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060257.1"
                     /translation="MIRSLLLVLALVGTAHARPASTGFYAEGGLGAVVPLPPDSDNAA
                     VGPSLNLRIGRDLFSWVSIGGYAAVSSHEATVPPPPEGEWFQLYRFGGELRLGGRADR
                     IAFFVEGGVGGTVISSNVLEKVGTTEPGEKFSITFHGGGGFEYQLENRHYAIGVAVDG
                     FYEPLFEQFKAIDSRLYLRYTYGGG"
     gene            complement(4926..6251)
                     /locus_tag="VFV99_12910"
     CDS             complement(4926..6251)
                     /locus_tag="VFV99_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5060258.1"
                     /translation="MTDERKWAERKVGFARLWARAKQVLTTDPTGSDEDDHVVDEEAA
                     RALAAQAGRLKGGLAKVAQLAAYHPGATLGRARGASAQARAVLGGLWDHVPAMAAGDI
                     AAVVEKDLGKSPQALFAHWDASPLAAASLGQVHAAKLDDGTDVVVKVQYPGVADKLRA
                     DLDDDDFIRKLAGAEIGRSLDTEALHTLGDAVRGELDYRAEAAAQMKFAAAWDGDKSL
                     RVPKVIGELSSQRVLTMTRAKGKPMVEIAASGAPDVRAQAAAAIYKFAWGSPLAHGLL
                     NADPNPGNFLVEVEADGHALVWCLDFGCALELPDAIRDLDREIWWGLLDDEAVTGAER
                     FRVALGRAGLLRRTDMLATTAHRDWESALAQPLATHGNFHWDADYAGRFAETTARVLD
                     AGGVQLPARVLLLWRQRLGVSAVIGMLDANAPFRRVLLDLVGTGKRALR"
     gene            complement(6244..8073)
                     /locus_tag="VFV99_12915"
     CDS             complement(6244..8073)
                     /locus_tag="VFV99_12915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5060259.1"
                     /translation="MTSNGRPDSQSGTVLPAPARRWIGHAFTIAIGLIGWFVVLGLPA
                     LGLIPSDPLAPALPIILFVVVILTARALAFRLVEGSVLSLDSAYYVAAALCVGTVEAG
                     RLVALALTLDASARLSAKQRRGDLDPDGWWAELGYVVYFGGMSGGLLVGCAWVFAGGG
                     VGHQADPVWVTLRVVAIASTLLVAHYALQGLRLRFLGHQWNEYLRQLALPGIVAEASL
                     MPIGAVLVLLYDPQQPLGFALLSLTYLLINFVFARLSRARHQLQERVHDLEILNATAR
                     RFSSSLQREELVEAVAREVCKAIPEAEAVTLVHRRTGRESELVIDAFDRGRDKFFRHP
                     MRPGEGAAGWVMKNGVARRIDDFTTSDVATDTPSHGMHSWLGAPLFMYGDCDGVVAVQ
                     SSRVGAFKPEHLALLESLALQIAAALQNAHLYELAMVDGLTGLFVRRYFDARIEEEIE
                     RSRRYGTPFSVIMLDVDDFKKLNDTYGHLVGDRVLRAIANVIKAQMRGVDTAARYGGE
                     EMSVILPRTEMVGAYNLAERIREAIAELRITTDDEPPRSLGVTASLGISAFPESKAAD
                     GVDLVRRADKALYRAKKTGKNRVELFWTDDPSGLHKLPELARD"
     gene            complement(8176..8352)
                     /locus_tag="VFV99_12920"
     CDS             complement(8176..8352)
                     /locus_tag="VFV99_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060260.1"
                     /translation="MLDPWIIEEILKKEEERRRESERGRAELPIERERDTGPRSTVTP
                     PREETERGVMIIDI"
     gene            complement(8728..9387)
                     /locus_tag="VFV99_12925"
     CDS             complement(8728..9387)
                     /locus_tag="VFV99_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060261.1"
                     /translation="MRLVLCICVVLGLGAAAACGRPRGPSQTLDKYGRALKNHDFGDA
                     YDLMSSGFRSKVSREDYVRMMRDNPREVDETADRLRGKHGSMEVTAEFEYGLGDQMRL
                     VQEGGRWRIASNPLGFYDQSTPKGALRSFIRAYRLERWEVMLRFVPNQYREKMDATKM
                     KAQFTGPSKEQMETLMNTLEANVDEPIIERGNDARMSYGDRFEVKFVKEDGVWKLKDL
                     D"
     gene            complement(9395..10720)
                     /locus_tag="VFV99_12930"
     CDS             complement(9395..10720)
                     /locus_tag="VFV99_12930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5060262.1"
                     /translation="MRRAGGPRILVALGLTNLIAYAMRNSLFAVYPDLRDLFGLRDAK
                     LGLLTTVFLIPHAIATLPFGWAGDRYDRRRVIALGMLLASVAGAAGALAQNTTQLAIS
                     RALLGFGTAAVVPVANSILGQLYEGPHKASRMAIFNLGLLLGGVAGFGVGAIAGFPAV
                     VVVLAIPGVVLALVMLTLPVPAHPAPHDKVSLPRYILQLGRLFVVESKELLRIRTLRW
                     LMVSTTAMAFAAGGFNAWLLDFLEREKAMTKESATSLLMVAMVGAVAGIIVGGRLSDR
                     LRSRFVAGRLWTIVIGMTLAIPCTIACLELQGVATFDLRTLTVGDYSFTWLTGHGVNP
                     LYYIAGTANFFFFSWYHAPMAATVDDLAPRDKLVAAQGLVIFTMHLFGTSSSSYVVGV
                     VSDHSSLYQAMWVPAGALVIAALAQLVATGSFAGDHLRARSGEAVRASL"
     gene            complement(10808..12145)
                     /locus_tag="VFV99_12935"
     CDS             complement(10808..12145)
                     /locus_tag="VFV99_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060263.1"
                     /translation="MKQYVVLAVAIALCVVACRGGGTKTKRTGSAAPVQVIDEPQLLD
                     AGHGPGPNADEIEPNDGDEVATPMSIGGTARGKIDPETDVDYYHLTIDKPGVLQVTLS
                     PVEGMDLSLELADASATTIAKSDRGAARVKEGLPNVGVTPGRYTLIVKQVKKKPAKPA
                     KPAKGRKAPEQEPPSAPVAVPVYELSTQMIDPGKGGEHEPDDDRGTANDLIVADNATG
                     FIGWSGDRDVWKLSVETLSDKNLLDVHVSAIEGIALELEISDGIGQPLATRKAPKGAA
                     LEMKGLMPNVPQGAPPFHYLTVRADKSNPETAYTLHATARIGGTDEEVEPNDLVDKPY
                     MMPTDRTVVHATWSPGDIDCFGLSPAGTARQIDVNVDTPGEINLDAELLVDGKSVAIA
                     NKGGKGVAEKLTAIVPANTRPVVRIKNPDANATVEAKYDVTIQESTATQDNAP"
     gene            complement(12142..12597)
                     /gene="fabZ"
                     /locus_tag="VFV99_12940"
     CDS             complement(12142..12597)
                     /gene="fabZ"
                     /locus_tag="VFV99_12940"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5060264.1"
                     /translation="MDAPGVLKAEQILGLLPHRYPFLLIDRVLELTEEKVIALKNVTF
                     NEPFFQGHFPGVPVMPGVLQIEAMAQAGGILASKAVQFDASTHVMLFMAIDAVKFRKA
                     VVPGDQLHIEVVPLRKGKIFKMKGEIKVDGQVVSQAEFLAGLAEKSKVQ"
     gene            complement(12667..13143)
                     /locus_tag="VFV99_12945"
     CDS             complement(12667..13143)
                     /locus_tag="VFV99_12945"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX5060265.1"
                     /translation="MVSPLLSGLATSAVPAPKIGVIDIDNTLSSTPAGKRASDAFEKT
                     RKTKQSELDKKQEELKKADADLDKQKAVLKPEVFAAKRQELEKKFVELQQTYVKLERE
                     LAGERAKLIQDLLKQASPKIEALAKAEGVNIIVDREAVVWADKSVDLTQKLNAEMK"
     gene            complement(13230..15785)
                     /gene="bamA"
                     /locus_tag="VFV99_12950"
     CDS             complement(13230..15785)
                     /gene="bamA"
                     /locus_tag="VFV99_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX5060266.1"
                     /translation="MIRVWLSVLVVAIVAQGAAAQPATGSGAGSGSAQAPAQAPAPAP
                     APAPTPPAQPAPQQPAPQPQAPPPSASGAVTWQGRPIERVQFRGNRKVEDDAIRVQLL
                     SKPGTLLDAAKLREDLRAMWKMGFFADIDVEGEVGTSGAITLTFAVKEKPAIRKVLVA
                     GNSDLGLDKINEVIDLELDAIVDISKVKKNREKIADLYVQKGFYLATVDYEIKPVNEA
                     EVDVWFKIDEKSKVKIREVQFIGNNNISDEELRNSIATRRADALSFLNDSGTYSQEAF
                     ERDLLLVSAHYWDRGYANVKVGTPQLRLSRDKQYMYLSIPVDEGPIFTIGAVNFKGDL
                     IGGAAKNLEKVKIRPGMIFSRTRIAEDRENLSAFYQDQGYAYANVLPLTKVDLDKRTI
                     SLTFEVSRGKRAYFERINIRGNSKTRDKVIRREMKISEGELFNNTNLEVSKRRIMALG
                     FFENVVVSTKRGSSDEFVEVNVEVTERPTGTFQIGAGFSSVENFIAQAQISQNNLFGR
                     GQTLALQAQISSLRQLFLLRFVEPYFLDTQWTFAFDLYNQSRGFGSFYRNASGGTLTW
                     GYPLSYEARAFLPYKLEDVSITTGSGGIASLGAVSAPIASTQVANLFRGGVTSSIRGS
                     LSWDSRNNRLFPTAGWYHSIFAEYASQYTGSENKFVRWGGFARHYRPIWGPFVLKINA
                     ELGVTTSTDPLGVPISERYLVGGIYNIRGYAPLSLGPKLRTQNPGDVGQNLSSLPLGG
                     NMQVIWNSEIEFPLFKKVGISGVVFYDMGNAYNLEDRYCSGLQRKNSQISIKFDPCFR
                     AAESLTTGLRKSVGFGFRWISPIGPLRFEWGIPLDAQPNEDPLVFEFTIGNFF"
BASE COUNT         2849 a         5163 c         5110 g         2741 t
ORIGIN      
        1 agatccagca gaaggcgctg acgctgttca agaccggcta cacgaaggcg atccagatgc
       61 aggtgtacga cgagtacacc gcgaagatcc gcgaggcgct cggtcggctc gacgccgatc
      121 acttcccgcc ggagaaggaa gggcgaagcc gcgaacgcag cgccgaccgt ccgctcaatc
      181 ccgacttgat gacggaggtg gcgcgatgaa gaagctcctc gtcatcgtgc tcgtcgcgct
      241 caccgcttgc ggtggctcga cgaagcgcac ggtccgcggt ggccgtacgg tcgaagccaa
      301 gcgcgagaag atcaaaccgg ccgcgatgaa ggagtacgag gccgcgatgc gcgcgctgcg
      361 gctcggcggc cccgaggcta acgaaacggc acgagctcgc ctgcgcagtg cggtcaagac
      421 tgattcgacg ctgtgggaag gctggtacaa cctcggcgtc atcgcgtggc gcgaaggcga
      481 cgacgacgaa gcgatcgacg actacggaaa ggcgctggcc cagaaccgcg accacacgcc
      541 gacgctgatg gcgcgcgccg aggcgtaccg ccgcgcgggt cgcaagaagg aagcgcgcgg
      601 cgactacgag gccgcgctca agggaatgga ggaagacgat cctaaccgcc gcgacgcggc
      661 agcgcgtctc gcctcgctgc tccgcgacgg cggcgattac gaggatgcgg tcgaggtact
      721 gcgcgagacc gtgcgcgtgt ccggcaccaa cgcacgcatc tacaccgagc tcggccagat
      781 ctacatcgcg cagaagcggc tcgagctcgc gcagctcgtg ctcgccaaag cgctcgagct
      841 cgacgcgaag gatccgtcgg tctacaacgc gctcgcactg ctcgcgcagc gccagggcaa
      901 atcgcaggaa gcgttcgagc gctacgacca ggcggtctcg ctcgatgcga agtacatcga
      961 cgcccggttc aacaaggcga ccgtgcttct cgatgccggc gactacttgc gagccaagac
     1021 cgagctcact gcgatcgtcg aaaacaaaca ggacgactat gcggcgtggg tgtcactcgg
     1081 cgttgcgcat cgcggtctcg cggagttccc cgacgcgagg aaggcatacg agcgcgtgat
     1141 caaggaagcg cccaagcgca gctccgcccg cgccgacgcg atgttcaact tcgcgatttt
     1201 gaagatggac tttctgaacg atcaggccgg cgccaagacc gagctggagc gatatctgca
     1261 ggaagcaccg acgagtcacg caaaacatca ggaggcggag acgctgtgca aacaactcaa
     1321 gtgccgttga ggcatggtgc ccccatgcgg gggctgctga ctacgctctt cgtgtgtgcg
     1381 ctggtcgcac ctgcagccgc gcaacccaag aagggtgaca aaggtccgcc gccccaagag
     1441 gcgaagcaga aggcgcccga aaaaggtgag aagaaggaga aggacaaagt gaagaacttt
     1501 gacttcaacg ccctcgacct gaacggccgc atgcgcacgc cgcagctgct ctacttcctc
     1561 gagcgcgcga acgaagagct cgagcgcgcg agcctcgaga agcgttcctt catcccgcac
     1621 atggtgcgga gcgtggagga agaacagctg tgagcacggt tgccctccgc acagcgctga
     1681 tctggcagga cgaagtcatg gatgacgtcg tcctccagaa gccgacgaag atcaccgtcg
     1741 gcagtgcggg aggctccacc ttcgtcgtgc cgaacgtcgg cctgccgaag aacttcgcca
     1801 tcgtgcggcc gggcaatcgc ggctacttgc tcacgctcgg tgagcggatg cgcggcacga
     1861 tctgcatcga cggcgaacag cgcgacgttg ccgagtacgt caagaagaac gtcgaacgtg
     1921 gcgggttcgc cgcgacgccg atcagcggca aagactgggg cgtcatcgat ctcgacgagt
     1981 ctggtgacta caaggtgttc ttccagttcg tgccgctcga cgatgcgacg gcacaaccgt
     2041 tcttcacgaa gcaggtgatc acggcaggca ttgccggcta cctgctgtcg atcgtgacgc
     2101 tcgcgatctt cttctacctg cgcggtgtct cgaccgattt cacgctgttc aattccgcgc
     2161 tgctcgccga attcgccgag gccggcttcc gcggctggtg catcgcgagc gcggcactcg
     2221 gcgtatccgc gatcggctgg tggctcgtcc agcaggaggg cgaccagcaa gcgtcgtttg
     2281 cattctcggt cttactccac gcggcgctgc tgttcatgac gtaccagctc tacgatggca
     2341 gcaatccgtt cgtgtggccc gggccgcgct cgctgacggg cagctatctc gtcacgcgcc
     2401 tcgagcccga gccggaaccg aagccggtac cgacggtcgc gacggtcggc cagaagaaag
     2461 acaccggcgc cgctgcaccg aagtcgcccg agaagccgaa gaatacggcg acgaagaacg
     2521 acgaaggtgc agccggtggc aaaggcgaga ccgagcgcgc gcgcgatccg aacgcgaagg
     2581 acgtgccgcc cgctccgcca aaggtcgcgc tgttcgagga caagaaccgc aaggtcctcg
     2641 acaacatcat cgatcgcaat ctggcgacga gcctcgacaa gttcaccggc atcaagggtg
     2701 acgaagtgaa gcgtggctcg ctcggttttg gcgaaggcac cggaactggc gtcggcgcag
     2761 gtgtcggcac cggcaccacg cgcggcagca acaagaacgg cagcggtggc ggcggtaacg
     2821 ccgagggcga cttcgtcagc aacaagggca agatcgacac cggcaaggac cgtccgggcg
     2881 gcggcaactg tgcgaagccg ccgtgcggca cggcgccgaa ggaagtgaag gtcgtgctgc
     2941 aggacgccga gggcgacttc ggtggtctca ccgctgaaga gatcaaccgc gtcgtgaagg
     3001 cgcgagctgg catcttccgt gcttgttatc agggcgagct caaccgcaac ccggggctcg
     3061 gcggcaagct tgtcgtcgcg ttccagatcg gcggtgacgg tgtcgtcaag agcgcgaaga
     3121 tcggtggtgg tagctcgatg cgtaacgaca acgtcgaagg atgcgtgaag tcgaacatca
     3181 tgcggctcaa gttccctgcc aaaggcggca tcgcaaatgt gaattatcca ttcctgttcc
     3241 aaccgggagg gtagtgatgc ggaagctcat cttcatggtc ctgatcaccg gctgcacgaa
     3301 cgagccgcag tacgtgtcgt gcgcgccgat gggcgcagca gcgatggatc tgtgcaagct
     3361 cgagggcggc atggacgatg gtatgggcgg cacgctcgac gaggtcaaag gcagcgtcca
     3421 catcccgctc aggcgcgagg aggattggaa gcagagcgat caagacaagc gcacggagct
     3481 gatggcgaac gcacccgcgg gcgtcgacgt gccgatctat cgtcccgagc actacgacat
     3541 ctccgtcgag tggacggtca agaacctgac ggaccagcag agtcagttcc gcgtcgatct
     3601 caatggcgcg aacgaggaat tcacctacga cccgtcgatg atcatcctcg atccgaccga
     3661 tgacgaagcg ccgccgacgc cgccgctcgc cggcaacatc ccgatcgata tcggtcccaa
     3721 cgccgagatc agcggcacgt tccgcgagga ccagctcgtc gaggcggcca tcgatctcga
     3781 tcaggtcacg cgcggcaacg tgaacccgtt ccgcgcggtg ctgacgacgt cccgccacga
     3841 cgaccagttc cagcccgtca cgccctacga ctacgtcaac atgacgggtg gcgagccaac
     3901 gggcccggca gtcccgagct acgcgtggcg gcagatcgtg cgcgtcgaca tcgtgttcaa
     3961 gccgcaggcg cacatgacga tcgactacac cgttcgcgtg cgcgagcacg tcgatgtcat
     4021 tcctgatgaa ggcctcaacg accccgacat caacgttttg gatccggcac cgtacgtccc
     4081 gtagctattt cgcttacgat agctcctgga tgattcgcag cttgctgctg gtgctggcgc
     4141 tggtggggac cgcgcatgca cgcccggcgt cgacgggctt ctatgccgag ggtggtctcg
     4201 gcgcggtcgt cccgttgcct cctgactcgg acaacgcggc cgtcggcccg agcctcaatc
     4261 tacgcattgg ccgcgacctg ttctcgtggg tttcgatcgg cggctacgca gccgtttcga
     4321 gtcacgaagc gacggtccct cccccgcccg agggcgaatg gttccagctc taccgcttcg
     4381 gtggcgagct ccgcctcggc ggtcgtgcgg atcgcatcgc gttcttcgtc gagggtggcg
     4441 ttggcggcac ggtgatctcg tcgaacgtgc tcgagaaggt gggcaccacc gagcccggcg
     4501 agaagttctc gatcacgttc cacggcggcg gcggtttcga gtaccagctc gagaaccgcc
     4561 actacgcgat tggcgtcgcc gtcgacggct tctacgagcc gttgttcgag caattcaaag
     4621 cgatcgattc gcggctctac ctccgctaca cgtacggcgg cggctaacgc attcgagctg
     4681 aagcagtcga tcgacgttca aaccagcgca cagccggtcc cgtcggcact ttcgcaaaag
     4741 agatcaccgc caagacgccg gggacgccaa gcgcagagat caggttacgc gcgcgtcctg
     4801 ctgccacgac cgttcgtgca gagcgcgatc caaaccctac ttctgatggc ggccttggcg
     4861 tctgcaggcg ccttggcgtt tatccggggc ggctatgcgc ctgacgctgt tgcgtgaacc
     4921 aagcgtcaac gcagcgcgcg tttcccggtg ccgaccagat cgagcagcac gcggcgaaac
     4981 ggcgcattcg catcgagcat gccgatgacg gcgctgacgc cgaggcgttg gcgccagagc
     5041 agcagcacgc gggctggcag ctgcacgccg cccgcgtcga gcacgcgcgc ggtcgtttcg
     5101 gcgaaccgtc ccgcatagtc ggcgtcccag tgaaagttac cgtgcgtcgc gagcggctgc
     5161 gcgagcgcac tctcccagtc gcggtgggcg gtcgtcgcga gcatgtcggt gcggcgcaag
     5221 agccctgccc gcccgagcgc cacgcgaaac cgctcggcgc cggtgaccgc ttcgtcgtcg
     5281 aggaggcccc accagatctc gcgatcgaga tcgcgaatcg cgtcgggcag ctcgagtgcg
     5341 cagccgaagt cgagacacca cacgagcgcg tgaccatcgg cttcgacttc gacgagaaaa
     5401 ttgcctgggt tcgggtcggc gttgagcagg ccgtgcgcga gcggcgagcc ccacgcgaac
     5461 ttgtagatcg cagccgctgc ttgcgcgcgt acgtcgggcg caccgctcgc cgcgatctcg
     5521 accatcggct tgcccttggc gcgcgtcatc gtgagcacgc gttgcgacga cagctcgccg
     5581 atcaccttcg gtacgcgaag cgacttgtcg ccgtcccacg ccgcagcaaa cttcatctgc
     5641 gcagcggctt cggcgcgata gtcgagctca ccgcgcaccg catcgccgag cgtgtgaagg
     5701 gcctcggtat cgagactgcg cccgatctcc gcgcccgcga gcttgcggat gaaatcgtcg
     5761 tcgtcgagat ccgcgcgcag cttgtcggcg acgccgggat actgcacttt gacgacgacg
     5821 tccgtgccgt cgtcgagctt ggcggcgtgg acctggccga gcgaagcggc cgcgagcggc
     5881 gacgcatccc agtgcgcgaa caacgcctgc ggcgacttac cgagatcctt ctcgacgacc
     5941 gccgcgatgt cacccgctgc catcgctggc acgtgatccc agaggccgcc gagcaccgca
     6001 cgcgcctgtg cactcgcgcc acgcgcacga ccgagcgtcg cgcccggatg ataagccgcg
     6061 agctgcgcga ccttcgcgag cccgcccttg agtcgcccgg cttgcgctgc gagcgcgcgt
     6121 gccgcttcct cgtcgacgac gtgatcgtcc tcgtcggagc cggtcgggtc cgtggtgagc
     6181 acctgtttcg cgcgcgccca cagccgtgcg aatccgacct tgcgctctgc ccacttccgt
     6241 tcgtcagtca cgcgcgagct ccggaagctt gtgcaggccc gaaggatcgt cggtccagaa
     6301 cagctcgacg cggttcttgc cggtcttctt cgcgcgatag agcgccttgt cggcgcggcg
     6361 cacgagatcg acgccgtcag ccgccttgct ctccggaaat gccgagatgc cgagcgatgc
     6421 ggtcacgccg agcgagcgcg gtggctcgtc atcggtggtg atgcgcagct cggcgatggc
     6481 ttcgcgaata cgctcggcga ggttgtacgc gccgaccatc tcggtgcgcg gcaggatcac
     6541 cgacatctcc tcgccgccat aacgcgccgc cgtgtcgacg ccacgcatct gtgccttgat
     6601 gacgttcgcg atcgcgcgca acacacggtc tccgacgaga tgcccgtagg tatcgtttag
     6661 tttcttgaaa tcgtcgacgt cgagcatgat cacggagaac ggcgtgccgt aacgccgcga
     6721 ccgctcgatc tcctcctcga tgcgtgcgtc gaagtagcga cgcacgaaca gcccggtcag
     6781 accgtcgacc atcgccagct cgtagaggtg cgcgttttgg agcgcggccg cgatctgcag
     6841 cgcgagcgac tcgagcaacg ccagatgttc gggcttgaac gcgccgacgc gcgacgactg
     6901 caccgcgact acgccgtcgc agtcgccgta catgaacagc ggtgcaccga gccaggaatg
     6961 catgccgtgg ctcggcgtat cggtcgcgac gtccgatgtc gtgaagtcgt cgatccgtcg
     7021 cgcgacgcca ttcttcatca cccagcccgc ggcgccttcg ccggggcgca tcggatgacg
     7081 gaagaacttg tcgcgcccgc gatcgaacgc gtcgatcacg agctcggact cgcgcccggt
     7141 gcgacggtgc acgagcgtga cggcctcggc ctcgggaatt gccttgcaga cctcgcgcgc
     7201 gactgcttcg acgagctcct cgcgctgcag cgacgacgaa aatctccgag cggtcgcgtt
     7261 caagatctcg aggtcgtgca cgcgctcttg cagctggtgc cgcgcacgcg acagccgcgc
     7321 gaacacgaag ttgatgagca gatacgtgag cgagaggagc gcgaacccga gcggctgctg
     7381 cggatcgtag agcaacacga gcactgcacc gatcggcatc agcgacgcct ccgcgacgat
     7441 gcccggcagt gcgagctgac gcagatactc gttccactga tggccgagga agcggagccg
     7501 caaaccttgc agcgcgtagt gcgcgaccag cagcgtcgag gcaatcgcga caacccgcag
     7561 cgtgacccaa actggatccg cctgatggcc aacgccgcct cctgcaaaca cccacgcgca
     7621 gcccacgagc aagccgccgc tcataccgcc gaagtacacg acgtaaccga gctccgccca
     7681 ccagccgtcg ggatcgaggt caccgcgccg ctgcttcgcg gagaggcgtg cggacgcatc
     7741 cagagtgagc gccagcgcca caaggcggcc cgcctctacg gtgcctacgc ataacgcggc
     7801 agcgacgtag tacgccgagt cgagtgacag cacgctgcct tcgacgaggc gaaacgcgag
     7861 tgcgcgggcc gtcaggatga cgacgacgaa caaaatgatc ggcaatgcgg gcgcgagtgg
     7921 gtcgctcgga atcaagccga gtgccggcaa gccgagcacg acgaaccaac cgatgagacc
     7981 aatcgcgatc gtgaacgcgt ggccgatcca gcgccgagcc ggtgcaggca gaacggtgcc
     8041 tgactgcgag tctgggcgac cgttactcgt caccgcgcgg atctatcaga tactgcgaca
     8101 gaagtcgcgg aaccccgaaa agcgcgaaag cccccgccac cgtaggtggg agggctttcg
     8161 gttgaggttg ataggtcaga tatcgatgat catcacgcca cgctcggtct cctcgcgcgg
     8221 aggagtgacc gtcgagcgcg ggccggtatc gcgttcacgc tcgatgggaa gctcagcccg
     8281 accccgttca ctctcacggc gtcgttcctc ttctttcttg aggatctctt cgataatcca
     8341 gggatccaac atggcggacc gcctttcatg ggacggatgg tcgagcctaa ctagttatct
     8401 ttgcgtagct cctatcctcc aacactgcaa cctgcgggcc ttgtgacgat gaatcccgca
     8461 cgttcattct aacgcatgga tatccgccag gctccacttc gcgcacgcga tttcgcaagt
     8521 cggcgagatc gactcaatca gggcaccgct cgggcctaca gccctcactg acgcacgggg
     8581 tcgtcgacgt ttatcgaacg cacgcgccac gttgtgctcg atctctacac ccttactttt
     8641 ttgcggagtg tgagtgacga atctggcacc tggcagatcc accaggtggg caaatcccca
     8701 catcggtggc agcccgaggc gccagctcta gtcgaggtct ttgagcttcc agacgccgtc
     8761 ttctttgacg aacttcactt cgaatcggtc gccatagctc atccgtgcgt cattgccgcg
     8821 ctcgatgatc ggctcgtcga cgtttgcctc gagcgtgttc atgagcgtct ccatctgctc
     8881 tttgctcggg cccgtgaact gcgccttcat cttcgtcgcg tccattttct cgcggtactg
     8941 attcggcacg aagcgcagca tcacttccca gcgctcgagt cgatacgcgc gaatgaacga
     9001 gcgcagcgcg cccttcggcg tcgactgatc gtagaagccg agcgggttcg aagcgattcg
     9061 ccagcggccg ccctcctgga cgagccgcat ctgatcaccg aggccgtact cgaactcggc
     9121 ggtgacttcc atgctgccgt gcttgccgcg caggcggtca gcggtctcgt cgacctcacg
     9181 cgggttgtca cgcatcatcc gtacgtagtc ctcacgcgag accttgctgc gaaagcccga
     9241 cgacatcagg tcgtaggcgt cgccgaagtc gtggttcttc agcgcgcggc cgtacttgtc
     9301 gagcgtctgc gacggtcccc ttggccggcc gcaggcagcg gcggccccga ggccaagaac
     9361 gacgcagatg cagagaacca agcgcacgac ggggttatag cgaagccctg acggcctcgc
     9421 cactccgggc tcggagatga tcgccggcaa agctccccgt cgcgacgagc tgcgcgaggg
     9481 ccgcaatcac gagtgcgcca gcaggtaccc acatcgcctg gtagagcgac gagtggtccg
     9541 agaccacgcc gacgacatag gacgacgacg aggtgccgaa cagatgcatc gtgaagatga
     9601 caagcccctg cgcggcaacc agcttgtcgc gcggcgcgag atcgtccacg gtcgcggcca
     9661 tcggcgcgtg gtaccagctg aagaagaaga agttcgcagt gcccgcgatg taatagagcg
     9721 gattgacgcc gtggccggtg agccacgtga acgagtaatc gccgaccgtc agcgtgcgca
     9781 ggtcgaacgt cgcgacgccc tgcagctcga ggcacgcgat cgtgcacgga atcgcgagcg
     9841 tcatgccgat gacgatcgtc cacaatcggc ccgcgacgaa tcgcgagcgc aggcgatccg
     9901 aaagccgacc gccgacaatg atgccggcga ccgcgccgac catcgcgacc atgagcagcg
     9961 acgtcgcgct ctctttggtc atcgccttct cgcgctcgag aaaatcgaga agccacgcgt
    10021 tgaagccgcc ggccgcgaat gccatcgccg tcgtcgacac catcagccag cgcagcgtgc
    10081 gaatgcgcaa gagctccttg ctctcgacga cgaacagcct gccgagctgc aggatgtagc
    10141 gcggcagcga caccttgtcg tgcggagccg gatgcgcggg caccggcagc gtgagcatga
    10201 ccagcgcgag caccacgccg gggatcgcga gcacgacgac gaccgctgga aagccagcga
    10261 tcgcgccgac gccaaacccg gcgacgccgc cgagcaagag gccgaggttg aagatcgcca
    10321 tccggctcgc cttgtgcggg ccttcgtaga gctggccgag gatcgagttc gcgaccggga
    10381 cgaccgccgc ggtgccgaag ccgagcagcg cgcgagagat cgcgagctgt gtcgtgttct
    10441 gcgcgagtgc accggcggcg ccggctacgc tcgcgagcag catgccgagc gcgatcacgc
    10501 gacggcggtc gtagcggtcg ccggcccagc caaacggcag cgtcgcgatc gcgtgcggga
    10561 tcaagaatac ggttgtgagc agaccgagct tggcgtcgcg caggccgaac aggtctcgca
    10621 ggtccggata gaccgcgaac agcgagttgc gcatcgcgta cgcgatcagg ttcgtcaggc
    10681 cgagcgcgac gaggatgcgc ggaccgccgg cgcgccgcat ccacagcgac tctcgcggtg
    10741 aaacctcaac cggatgtccg ctgctcatcg cccacctgcc acgatagcag ccctgcccga
    10801 gcgcgtgtca tggcgcgttg tcttgggtcg ccgtcgattc ctggatcgtc acgtcgtatt
    10861 ttgcctcgac ggtcgcgttc gcgtcggggt tcttgatgcg cacgaccgga cgcgtgttcg
    10921 ctgggacgat cgcggtcagc ttctcggcga cgcctttgcc acccttgttc gcgatcgcga
    10981 ccgacttgcc gtcgacgagt agctcggcgt cgagattgat ctcgccgggc gtgtcgacgt
    11041 tgacgtcgat ctggcgcgcg gtccccgcgg gtgaaagccc gaagcaatcg atgtcgcccg
    11101 ggctccacgt cgcgtgcacg accgtgcgat cggtcggcat catgtacggc ttgtcgacga
    11161 gatcgttggg ctcgacttcc tcgtcggtgc caccgatccg cgcggtcgcg tgcagcgtgt
    11221 acgcggtctc cgggttcgac ttgtccgcgc gcacggtcag atagtgaaac ggcggcgcgc
    11281 cctgcggcac gttcggcatc agccctttca tttcgagcgc tgcgcccttc ggggccttgc
    11341 gcgtcgcgag gggttggccg atgccgtcgc tgatctcgag ctcgagcgca atgccctcga
    11401 tcgcgctgac gtgcacgtcg agcagattct tgtcggacag cgtctcgacc gacagcttcc
    11461 agacgtcgcg atcgcccgac cagccgatga atccggtcgc gttgtcggcg acgatgagat
    11521 cgttcgcggt tccgcggtcg tcatcgggct cgtgctcgcc gcccttgcct ggatcgatca
    11581 tctgcgtcga cagctcgtag accggaaccg cgaccggcgc cgacggcggt tcttgctcgg
    11641 gagccttgcg gcccttcgct ggcttcgccg gtttcgcggg cttcttcttc acctgcttca
    11701 cgatcagcgt gtagcggccc ggcgtcacgc cgacgttcgg caggccttcc ttcacacggg
    11761 cggcgccgcg atccgatttc gcgatcgtcg tggccgacgc atcggcgagc tcgagcgaca
    11821 agtccatgcc ctcgaccggc gacaacgtga cttgtagaac cccgggcttg tcgatcgtca
    11881 ggtggtagta gtcgacgtcg gtctccggat cgatcttgcc gcgcgcggtg ccgccgatcg
    11941 acatcggggt cgcgacctcg tcgccgtcgt tcggctcgat ctcgtcggcg ttcgggccgg
    12001 ggccgtgacc ggcatcgagc aattgtggct cgtcgatcac ctggaccggc gccgcactcc
    12061 ccgtgcgctt cgtcttcgtg ccgccacctc ggcacgcgac gacgcacaga gcgatcgcta
    12121 cggcgagcac aacgtactgc ttcattgaac cttcgacttc tccgcgagcc cggccaggaa
    12181 ctctgcttgt gaaaccacct ggccgtcgac tttgatttca cccttcatct tgaagatctt
    12241 gcctttgcgc agcggcacga cttcgatgtg cagctggtcg cccggcacca ccgctttgcg
    12301 gaacttcacg gcatcgatcg ccatgaacag catgacatgc gtcgacgcgt cgaactggac
    12361 tgctttggag gccaggatgc cgccagcttg cgccattgcc tcgatctgca agacgcccgg
    12421 catcaccggg acgccgggga agtgcccctg gaagaacggc tcgttgaacg tgacgttctt
    12481 gagcgcgata accttctcct cggtcagctc gaggacccgg tcgatcagca agaacggata
    12541 gcgatgaggg agcagaccga ggatctgctc tgccttcagt acgcctggcg catccatgcg
    12601 ccggagacta tcccgcccac cccgtcgggg ccactgtcac gctggccgcg ataggcggcc
    12661 gcgcgatcac ttcatttcgg cgttgagctt ctgcgtgagg tcgacggact tgtcggccca
    12721 caccaccgcc tcgcggtcga cgatgatgtt gacgccctcg gccttcgcga gcgcctcgat
    12781 cttcgggctc gcctgcttca ggagatcctg gatgagctta gcgcgctcgc cggcaagctc
    12841 gcgctcgagc ttgacgtagg tctgctgcag ctcgacgaac ttcttctcga gctcctggcg
    12901 cttcgccgcg aacacctcgg gcttcagcac ggccttctgc ttgtcgagat cggcgtcggc
    12961 cttcttgagc tcttcctgct tcttgtcgag ctcgctctgc ttcgtcttcc gcgtcttctc
    13021 gaacgcgtcg ctggcgcgct taccggccgg cgtcgacgac agcgtgttgt cgatgtcgat
    13081 cacgccgatc ttcggcgcag gaacggcact cgtcgccaga cccgacagga gcgggctcac
    13141 catcaagcct agaccgagac aaccaagggc tagataccgg ttcatgggca cctcatacga
    13201 cgctttggac gagcggccaa cgggattgtt cagaagaagt tgccgatcgt gaattcgaaa
    13261 accaggggat cttcgttagg ttgcgcgtcc agcggtatgc cccactcgaa tcggaggggg
    13321 ccaatcggcg agatccagcg gaaaccgaag ccgactgatt tacgtaggcc ggtcgtgagc
    13381 gactcggctg cgcggaaaca cggatcgaac ttgatcgaga tctggctgtt cttgcgctgc
    13441 aggcccgagc agtagcggtc ctcgaggttg tacgcgttgc ccatgtcgta gaagaccacg
    13501 cccgagatgc cgaccttctt gaacagcggg aactcgatct cactgttcca gatcacctgc
    13561 atgttgccgc cgagcggcag gctcgacagg ttctggccga cgtcgcccgg gttctgcgta
    13621 cgcagcttcg gaccgagcga gagtggcgcg tacccgcgga tgttgtagat accgccgacg
    13681 aggtagcgct cgctgatcgg cacgccgagc ggatcggtcg acgtcgtgac gccgagctcg
    13741 gcgttgatct tgaggacgaa cggtccccag atcgggcggt agtggcgagc aaaaccgccc
    13801 caccgcacga acttgttttc ggagccggtg tactggctcg cgtactcggc gaagatcgag
    13861 tggtaccagc cggccgtcgg gaacaggcgg ttgttacgcg agtcccagga tagcgagccg
    13921 cggatcgacg acgtcacgcc gccgcggaac agattcgcga cctgcgtcga cgcgatcggt
    13981 gcgctcaccg cgccgaggct tgcgatgccg cccgagccag tcgtgatgct gacgtcctcg
    14041 agcttgtacg ggaggaatgc gcgcgcttcg tacgagagcg gatagcccca cgtgagcgtg
    14101 ccacccgatg cattgcggta gaacgagccg aagccgcggc tctggttgta gaggtcgaac
    14161 gcgaacgtcc actgggtgtc gaggaagtac ggctcgacga agcggagcaa gaacagctgg
    14221 cggagagagc tgatctgcgc ctgcaaggcg agcgtctggc cgcggccgaa caggttgttc
    14281 tgcgagatct gtgcttgagc gatgaagttc tcgacgctcg agaagccggc gccgatctgg
    14341 aacgtgccgg tcggccgctc ggtaacctcg acattgactt cgacgaactc gtcggagcta
    14401 ccgcgcttcg tcgagaccac cacgttctcg aagaagccga gcgccatgat gcggcgcttg
    14461 gagacctcga ggttcgtgtt gttgaacagc tcgccctcgg agatcttcat ctcgcggcga
    14521 atcaccttgt cgcgcgtctt gctgttgccg cggatgttga tgcgctcgaa gtacgcacgc
    14581 ttgccgcgcg acacctcgaa cgtgagcgag atggtccgct tgtcgagatc gaccttcgtc
    14641 agcggcagca cgttcgcgta cgcatagccc tggtcctgat agaacgcgga caggttctcg
    14701 cggtcctcgg caatgcgcgt gcgcgagaag atcatgcccg ggcggatctt cactttctcg
    14761 aggttcttcg ccgcgccgcc gatcagatcg cctttgaagt tgaccgcgcc gatcgtgaag
    14821 atcgggccct cgtcgacggg aatcgacagg tacatgtact gcttgtcgcg ggacagtcgc
    14881 agctgcggcg tgccgacctt gacgttcgcg tagccgcgat cccagtagtg cgcgctgacg
    14941 aggagcaagt cgcgctcgaa tgcttcctgg ctgtaggtgc cgctgtcgtt caagaagctg
    15001 agcgcatccg cgcgccgcgt tgcgatcgag ttacgtagct cctcatcgga gatgttgttg
    15061 ttaccgatga actgaacctc gcggattttg accttcgatt tctcgtcgat cttgaaccag
    15121 acgtcgacct cggcttcgtt gaccggcttg atctcgtagt cgacggtcgc gaggtagaag
    15181 cccttctgaa cgtagaggtc cgcgatcttc tcgcggttct tcttcacctt cgagatatcg
    15241 acgatcgcgt cgagctcgag gtcgatcacc tcgttgatct tgtcgaggcc gagatcgctg
    15301 ttgcccgcga cgagcacttt gcgaatcgcc ggcttctcct tcaccgcaaa cgtcagcgtg
    15361 atcgcgccgc tcgtgccgac ctcgccttcg acgtcgatgt cggcgaagaa gcccatcttc
    15421 cacatcgcgc gcagatcttc gcgcagcttc gcagcgtcga gcagcgtgcc cggcttgctc
    15481 agcagctgga cgcgaatcgc atcgtcctcg accttgcggt tcccgcggaa ctgcacgcgc
    15541 tcgatcggcc ggccttgcca cgtgaccgcg ccactcgcac tcggcggcgg cgcctgcggt
    15601 tgtggcgcgg gttgctgcgg cgcgggttgc gcgggtggcg tcggtgctgg tgctggtgcc
    15661 ggcgctggcg cttgcgctgg cgcttgcgcg gagccactgc ctgctccgct tccggtggcg
    15721 ggctgtgcag ccgcaccctg cgccacgatc gcgaccacga gcacgctcag ccatacgcgg
    15781 atcacgactg cacttccttc gcgggtgtca cgtccgtcgc cggtgcggca ctgtcttcgc
    15841 tcgcgtcggc atcgctcgga tcg
//
LOCUS       DASUFX010000322        11212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1437348, whole genome shotgun sequence.
ACCESSION   DASUFX010000322 DASUFX010000000
VERSION     DASUFX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437348"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFV99_12955"
     CDS             <1..890
                     /locus_tag="VFV99_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5060267.1"
                     /translation="GRDDGARVPPDAAAIAEALRGALDDTMLANLLQSIDLGLFAEGM
                     PGLRGDLRVVPLAEVLQLLDVQEQSGVLTVERSGARVDIYFRRGRVDQAIAEGVPDEF
                     LLGRFVTDAELMTRGDFEAFMESRLRGSQPGVSAHKLLGQSLVKLGHLAETDLRACLT
                     RQSSELIYEILRWRHGRFRFSAGMELPPSVIDAALGLDVEAVLMEGYRRVDEWHLIER
                     AIDNFDVVFLRNEDSVAQMGRGRLTREELAVLELVNGKNSVKDIIRKSRMGSFEVSKM
                     LYRLLSIKLVRRRVLPVAV"
     gene            complement(1103..1408)
                     /locus_tag="VFV99_12960"
     CDS             complement(1103..1408)
                     /locus_tag="VFV99_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060268.1"
                     /translation="MRYALLILLLACGSKSNPPSEASNKTPSPANDCITTGCSGTVCT
                     EPGKEVVTTCEYKPEYGCYKDGVCARQNDGSCGWTQTPSLQACLANPPALPVGGTPQ"
     gene            complement(1512..4316)
                     /locus_tag="VFV99_12965"
     CDS             complement(1512..4316)
                     /locus_tag="VFV99_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060269.1"
                     /translation="MRFRTLALAAALSVGISHTGDAFAPHKGADRQVVAAGRAPRLHR
                     DVKWTAPQGALAGLPGWRVQWDHDTDVPLRLWGQSIAAPGTSANANAAETFARQMLAE
                     HVALLSPGATASDFELVSNQLNPSGEIRTVGFAQRAFGLSVVGGAISFAFSHDRLMMI
                     SSTALPKVNVRMPQTSLPVSTVASAAKSFLSQAGYSVDVKAYGDRLIVPIVHSRGNRA
                     GVDIEYRVVETVTVESNRDAGSWNVWIDSNNAAPVARENLLHYATGKVLYDAPDRWPG
                     GTRSAKAAPYANHSIAGTATLSTLDGTITWASGSVTVTPGLVGQYVKITNKQGALATD
                     SLTLNDGGTVTWSRASVEYDDAEIITYIAASTAKEFVRTRLNPNLAWLNNLLSATVNE
                     SQTCNAYSTGNDIHFYRKSATTAQTQCENTGRLTDVVYHEFGHSVHAQSIIPGAGAFD
                     GSLSEGMGDTLAASITRDHGMGRGFFMNDEALRELDPVGTEKKWPDDADGEPHDEGEI
                     IGETLWDLRKGLIAKLGDQAGEDRLLKIFYGIIQRASDIPSSYAEALVADDDDGNLAN
                     GTPNQCVINTAFGLHGLADPTVTIGLEPPTRDGYKVSITIRPPTQTECPPPNVTGAKL
                     TYTPKGGNGTEIDLAASGDVWSASIPTQPDGTVVLYHVTVTLSDGSSLTYPQNPADPE
                     YQMYVGNVEKIWCADFEGGLGDWTVGGTTNRVEFEVGAPMGLAGDPKSAYAGTNVLGI
                     DLGSDDGLYNPRTTQYAQSPEIDLQGHTDVRLQYYRWLNVEDGVYDPATISVNDQIVW
                     QNKTSDMQSQTEINHTDKEWRFQDVDLAQYAASGKMTLKFQLVSDQGLELSGWNVDEV
                     CLVRPGAPSQLCGNGNVDTDEACDDGNTTDGDGCSATCQTEGTDDGGCCSAGTNPAGP
                     LALSVLTLGLVVLRRRQRRA"
     gene            complement(4430..5359)
                     /locus_tag="VFV99_12970"
     CDS             complement(4430..5359)
                     /locus_tag="VFV99_12970"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5060270.1"
                     /translation="MEVFRGHQQVPGWHNGAAVAIGNFDGVHVGHRALIRRARELAGA
                     HGAKTVALTFDPHPSVLLAPKPPAMISSLSRRIELLAEAGADVVVVEPFTRDLASRSA
                     HAFIDDIVISALCARAIVVGYDFSYGQGRSGTTDALRAHGGRAGVEVAIVAAVEIDGE
                     VASSSKIRSHLREGDLVAANRLLGRAWDIDGTVIHGAKRGRAIGVPTANIRTDTELLL
                     RTGIYAVTLEVDGRSLPAVASLGTNPTFVENGGVVLEVHLLDFDGDLYGKRTRTTFVS
                     RLRDEAKFDSIDALIEQIQRDIDAARRVLTSPV"
     gene            complement(5375..6241)
                     /locus_tag="VFV99_12975"
     CDS             complement(5375..6241)
                     /locus_tag="VFV99_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060271.1"
                     /translation="MVPPKTWLAIPIMAIACAAVAALLGEQPSAAVAIGALAAAAAAA
                     VRAFAGASVAAATTASAAALLVALGVLDLPHFDVPRATLAAAAALFAIAELARPLPVD
                     ASPLPAIGAALLAGVLDPAYVALLAIAGIRLLTGPWSRPRWTVLVPIVGMLAIGIAVL
                     AACAERGVFAELWHTWAARTGTHAPLTVLTQAADTLGPIATIAALAGLAVCALRGRYA
                     AAATLAVAIGAVAVDLAVGSLGAATLAIAAVGAGVGLARLAATVRWPTAQTFVGATAG
                     FLVVVAAAMLRL"
     gene            complement(6281..7534)
                     /locus_tag="VFV99_12980"
     CDS             complement(6281..7534)
                     /locus_tag="VFV99_12980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5060272.1"
                     /translation="MTLQETPKSGFFAVFRSKRMVVLFLLGFAGGLPLYLTSQTLQAW
                     MTAVGVERGRIDDVAAFGLAYTLKVLWAPLLDRYQLPFLGRRRGWLLAFQLLLVVTTA
                     IMALSDPRAHPYELAAIAIAVAFCSASQDIIIDAYMTDTLAEQERAAGSAAYVLGYRV
                     AVLMSGTLALIAADHLPWRTIYFGMAGLMSLGVIATLVADEPPERVAPPRTFADALVL
                     PFREFWQRLGTRGLVVVIGFAALYRFGDSFAQSLIIPFLKDGVKFSFTEIGVVFKGIG
                     FAGLAIGGAFAGALVARFGLRRMLVTFGILSAITNLLYSWLAVSGHDISVFCAAVFVD
                     QLSYALSTTAFLSVLMGSCTPAVSATQMALLTSLSSVGQRVFGPLADHVVTSLAWSGF
                     FLATALMAIPGLVLAWWVARHQPNG"
     gene            7580..9589
                     /locus_tag="VFV99_12985"
     CDS             7580..9589
                     /locus_tag="VFV99_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5060273.1"
                     /translation="MVHSSKRAKLAAAVRWLAPSAAAAAGGAALAGALEGAGMHGLFG
                     SAAAAGFVALVAFPALLAASAATRGLWHAWQPRALGMIDDGGGAPRLAGWFAFVWLAT
                     LALAWVMFQGTWLLAGATAFKPNGMAFAEPVLAVITVLLLVALSRPTARGLTWMYRKI
                     DQRFRMTPLRIIASLVIKTVIIAYLVWHFFAAKRIGALDFSILHVPLAGVMATAIVHV
                     VWERATRVRRIVGGVLGAVAAVLIGCALVAWQTRPALTLQIWGERPIAGLAIDKLFDL
                     DSIRAGVSLSEFRPIEKAGSPHPDIILITIDTVRADHTPPYGGTAEMPLLRELAVRGA
                     VYDWAFAPSNVTRRSIPSMVTGLAPNRVRGRVVGWALRIDPRHVVLAERMLAGGYETA
                     GFVCCYGFWGHDFRTGLQRGLSHLEIEPSGIKLAKQARQWLEAREKQPNKKPLFLWMH
                     ILEPHNWQQGQGTPQNDEDRRRLYDRSLTAADTMMVELLTSLSARKPEDAPIVIITAD
                     HGEALGEHGQPFHSTDLYNSQIRVPFVVAGPGIKPGRISETVSLTDLTPTVLELAGFV
                     PPTGQSMDGRSVADLATGRRLSTEDGGIAFSAMIRDRSNPGGITSLIRGRWKMIDSGN
                     SLELYDVHADPNETSNLATTRPQVYNELRGILQQYVKRGGESPFE"
     gene            9602..9952
                     /locus_tag="VFV99_12990"
     CDS             9602..9952
                     /locus_tag="VFV99_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060274.1"
                     /translation="MSVAVCPMCHRSNRPSAKECDDCGYEFGQSIEALRGMLQNQLLN
                     TRTMFWVLVAANLALAAVVAIGVMYGFMILPMLPFLFITYQSVRAGQKIAVTKHSLRL
                     IEQKQLPRATLVSG"
     gene            complement(9944..>11212)
                     /locus_tag="VFV99_12995"
     CDS             complement(9944..>11212)
                     /locus_tag="VFV99_12995"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5060275.1"
                     /translation="FSVGILLAEMVMARRLFTSTNDLDILLMVRDARLDRLHKYASEF
                     PVELRVLTVRALQRRPEDRWQTAAQFRDALDEWIRRTTRVGQRDLANLIGRVINSPTA
                     DLEGQPRQAAVDEGPSGLSGPMTRMHQAQAELEAQQARAEFIANSGVPEPAVPDPTID
                     MGSSGTMTISEDDIMPEQPREAASGPREVGDLREQSVLRLVYKLARTKGTGLLTLEGR
                     ASVLKEAYFLDGQPQFVNSNVESERLGNFLLEQKALSPQALQRALSVMHHFGGRLADT
                     LVGLDLLDPLEAYRLLAKQVAAKLMEAFSWQKGRYTWTPRQTNPYKARSLHLDAFRVI
                     GAGAAQLGEPLVEEWLQAQQRAFVAREPAQESELAQFGLGEALMRVYSLLDGRTRLGD
                     LAARVRSPEARINLLRLTYLLVQTDFARLS"
BASE COUNT         1843 a         3713 c         3847 g         1809 t
ORIGIN      
        1 ccgggcgtga cgacggcgca cgggtcccgc ccgatgctgc ggcgatcgcc gaggcgctgc
       61 gcggcgcgct cgacgacacg atgctcgcga acctcttgca gtcgatcgat ctcggcctct
      121 tcgccgaggg catgcccggc ctgcgcggcg accttcgcgt cgtgccgctc gccgaggtgc
      181 tgcagctgct cgacgtgcag gagcaatccg gtgtgctcac cgtcgagcgc tccggcgccc
      241 gcgtcgacat ctacttccgg cgcggccgcg tcgatcaagc gatcgccgaa ggcgttcccg
      301 acgagttctt gctcgggcgc tttgtcaccg acgccgagct gatgacgcgc ggcgatttcg
      361 aggcgttcat ggagtcgcgc ctgcgcggat cgcagcccgg cgtgtcggcg cacaagctgc
      421 tcggtcagag cctcgtcaag ctcggccacc tcgccgagac cgatctgcgc gcgtgcctca
      481 cgcgccagtc gagcgagctc atctacgaga tcctgcgctg gcgtcacggc cgcttccggt
      541 tttccgccgg catggagctg ccaccgtcgg tgatcgacgc tgcgctcggc ctcgatgtcg
      601 aagccgtgct gatggagggc taccgtcgcg tcgacgagtg gcatctcatc gagcgcgcga
      661 tcgacaactt cgatgtcgtg ttcctgcgca acgaagactc ggtcgcccag atgggccgcg
      721 gccggctgac gcgcgaagag ctcgcggtgc tcgagctggt caacggcaag aactccgtga
      781 aggacatcat tcgcaagtcg cgaatgggca gcttcgaagt cagcaagatg ttgtaccggc
      841 tgctcagcat caagctcgtg cgccggcgcg tgctcccagt cgccgtctag gcgtgcgtag
      901 agcacggcgt cgaagcgccc gcgtctgccg cttcgcggct cccagagaaa acgccaagaa
      961 gccaagaacg ccaagaggaa agttttcggg atcgcgttct gctcgtacga ccgccgctgc
     1021 agaccgcgtc gaagacccca cccatctggt ggcgtcctcg gcggcaaggc ttcttggcat
     1081 gagatctgga gcacgtcgtg cgttactgcg gcgtgccgcc caccggcagc gccggcggat
     1141 tcgcgaggca agcctgcagc gacggcgtct gcgtccagcc gcagctgcca tcgttctgac
     1201 gcgcgcacac gccgtccttg tagcagccgt actccggctt gtactcgcac gtcgtcacga
     1261 cctctttgcc aggctcggtg cacaccgtgc cgctgcagcc ggtcgtgatg cagtcgttcg
     1321 ccggcgacgg cgtcttgttc gacgcttcgc tcggcgggtt cgacttcgag ccgcacgcga
     1381 gcagcaggat cagcagcgcg tatcgcatgc gcggcactct agcagccgta acagagccga
     1441 aaaggacagc tggcgcagcg ccgcgtgatg agagggtgag gcgcggcgcc gcgccagtgt
     1501 tgaacgattg gctacgcgcg acgctggcgg cgacgcagga ccacgaggcc gagcgtgagc
     1561 accgacagtg cgagcgggcc cgcggggttc gtgcccgcgc tgcagcagcc gccgtcgtcc
     1621 gtaccttcgg tctggcacgt cgccgagcag ccatcgccgt cggtcgtgtt gccgtcgtcg
     1681 caggcctcgt cggtgtcgac gttgccgttg ccgcacagct gcgagggcgc gcccggacga
     1741 acgaggcaga cttcgtcgac gttccagccg gaaagctcga ggccctgatc gctgaccagc
     1801 tggaacttga gcgtcatctt gccgctcgcc gcgtactgcg cgaggtcgac gtcctggaac
     1861 cgccactcct tgtcggtgtg gttgatctcg gtctgcgact gcatgtcgct cgtcttgttc
     1921 tgccagacga tctgatcgtt gacgctgatc gtcgccgggt cgtagacgcc gtcctcgacg
     1981 ttcagccagc ggtagtactg caggcgcacg tcggtgtgac cctgcagatc gatctcgggc
     2041 gactgcgcgt actgcgtcgt gcgcgggttg tagagaccgt cgtcgctgcc gaggtcgatg
     2101 ccgagcacgt tggtgccggc atatgcgctc ttcggatcgc cggcgaggcc catcggcgca
     2161 ccgacttcga attcgacgcg gttcgtggtg ccgccaacgg tccaatcacc gaggccgccc
     2221 tcgaagtccg cgcaccagat cttctcgacg ttgccgacgt acatctggta ctcggggtcg
     2281 gctgggttct gcgggtacgt cagcgacgag ccgtcggaga gcgtgaccgt cacgtgatag
     2341 agcacgacgg tgccgtcggg ctgggtcggg atgctcgcgc tccacacgtc gccgctcgcg
     2401 gcgagatcga tctcggtgcc gttgccgccc ttcggcgtgt acgtgagctt cgcgccggta
     2461 acgttcggcg gcgggcactc ggtctgcgtc ggcggacgga tcgtgatcga aaccttgtaa
     2521 ccgtcgcgcg tcggcggctc gaggccgatc gtgaccgtcg ggtcggcgag accgtggagg
     2581 ccgaacgccg tgttgatcac gcactggttc ggcgtgccgt tcgcgaggtt tccgtcgtca
     2641 tcgtcggcga cgagtgcctc ggcgtagctc gacgggatat ccgacgcgcg ctggatgatg
     2701 ccgtagaaga tcttgagcag gcggtcttcg ccggcctggt caccgagctt cgcgatcagg
     2761 cccttgcgga gatcccatag cgtctcgccg atgatctcgc cttcgtcgtg cggctcaccg
     2821 tcggcgtcgt ccggccactt cttctcggtg ccaaccggat cgagctcgcg gagcgcttcg
     2881 tcgttcatga agaagccgcg gcccatgccg tggtccctcg tgatcgaggc agcgagcgtg
     2941 tcgcccatgc cctcgctgag cgagccgtcg aacgcgccgg cgcccgggat gatcgactgc
     3001 gcgtgaaccg agtgaccgaa ctcgtgatag acgacgtccg tcaagcgccc ggtgttctcg
     3061 cattgcgtct gcgccgtcgt tgccgacttg cgatagaagt ggatgtcgtt gccggtggag
     3121 tacgcgttgc acgtctgcga ctcgttcacg gtcgccgaca gcaggttgtt cagccacgcc
     3181 aggttcgggt tgaggcgcgt gcggacgaac tccttcgcgg tgctggccgc gatgtacgtg
     3241 atgatctcgg cgtcgtcgta ctcgacgctc gcgcggctcc acgtcaccgt gccgccgtcg
     3301 ttgagcgtca gcgagtcggt cgcgagcgcg ccctgcttgt tcgtgatctt gacgtactga
     3361 ccgacgaggc ccggcgtcac cgtcacgctg ccgctcgccc acgtgatcgt gccgtcgagc
     3421 gtgctgagcg tcgcggtccc ggcgatcgag tggttcgcgt acggcgcggc cttggcgctg
     3481 cgcgtgccgc ctggccagcg atccggtgcg tcgtacagca ccttgccggt ggcgtagtgg
     3541 agcaggttct cgcgcgcgac cggcgcggcg ttgttcgaat cgatccagac gttccaggag
     3601 cccgcgtcgc ggttcgactc gacggtcacg gtctcgacga cgcgatactc gatgtcgacg
     3661 ccggcacggt taccgcgcga gtgcacgatc ggaacgatga ggcgatcgcc gtacgctttg
     3721 acgtcgaccg agtagccggc ctgggacagg aagctcttgg cggcgctcgc gacggtcgac
     3781 accggcagcg aggtctgcgg catgcgcacg ttgaccttcg gcagcgccgt cgagctgatc
     3841 atcatgagtc ggtcgtgcga gaacgcgaac gagatcgcgc cgccgacaac cgacaggcca
     3901 aacgcgcgtt gcgcgaaacc aaccgtgcgg atctcgccgc tcgggttcaa ttggttggac
     3961 acgagctcga agtcgctcgc agtcgcgccc ggtgacaaca gcgcgacgtg ctcggcgagc
     4021 atctgccgtg cgaacgtttc ggcggcgttc gcattggcgc tcgtgcccgg tgctgcgatc
     4081 gattgccccc acagacgcaa cggcacgtcc gtgtcgtgat cccactggac gcgccagccg
     4141 ggcaaaccgg cgagcgcgcc ctgcggcgcc gtccatttca catcgcggtg cagacgcgga
     4201 gcgcgacctg cagcaacgac ctgacgatca gcgcctttgt gtggagcgaa cgcgtcgccg
     4261 gtgtgactga taccgacgga aagtgcggcc gcgagtgcga gggttcgaaa acgcatggag
     4321 tctccccttg atggcgaggg gcataaaaca cgaggccgca gctaggtcca acgcgggcca
     4381 gagcacatcc actgacttgc ctgagaccgc aggtagatag ctcggcgcat cacacaggcg
     4441 atgtcagtac gcggcgcgct gcgtcgatat ctcgctgaat ctgctcgatg agtgcatcga
     4501 tggagtcgaa cttggcctcg tcgcgcagcc gcgacacgaa cgttgttcgc gtccgcttgc
     4561 catagagatc cccgtcgaag tcgaggaggt ggacctcgag gaccaccccg ccgttctcca
     4621 cgaacgtagg gtttgttccg aggctcgcca cggcgggaag ggagcggcca tcgacctcga
     4681 gcgtgaccgc gtagataccc gtccgcagca ggagctcggt atcggtcctg atgttcgcag
     4741 tcggcactcc gatcgcgcgg ccgcgcttcg cgccgtggat cacagtgccg tcgatgtccc
     4801 aggcgcgtcc cagcaggcga tttgctgcga cgagatcgcc ttcgcgtaga tgactgcgga
     4861 tcttgctcga cgatgcaacc tcgccgtcga tctcgacggc agcgacgatc gcgacctcga
     4921 cgccagcgcg gccgccgtgc gcgcgcagcg catccgtcgt gccgctgcgg ccttgcccgt
     4981 acgagaagtc gtagccgacg acgatcgcgc gagcgcacag cgcgctgatc acgatgtcgt
     5041 cgatgaatgc gtgagcggag cggctcgcga gatcgcgcgt gaacggctcg acgaccacga
     5101 cgtctgcgcc ggcctcggcg agtagctcga tgcggcggct gagcgacgag atcatcgcgg
     5161 gtggcttcgg cgcgagcagc accgacggat gcgggtcgaa cgtgagcgcg accgtcttgg
     5221 cgccgtgcgc accggcgagc tcgcgcgcgc ggcggatcag cgcacgatgc ccgacgtgca
     5281 cgccatcgaa gttgccgatc gcgacagccg cgccgttgtg ccagcccggc acctgctgat
     5341 ggccgcgaaa aacttccacg tgcgaagaat acaactacag ccgcagcatc gcggccgcga
     5401 cgacgaccag aaatccagcg gtcgccccga cgaacgtctg cgcggtgggc cagcgcacgg
     5461 tcgccgcgag ccgagcaagc ccgacgcccg cgccgaccgc tgcgatcgcg agcgtggcag
     5521 caccgagcga gccgacggcg agatcgacgg cgactgcgcc gatcgcgact gcgagtgtcg
     5581 ccgcagcggc gtagcggccg cggagtgcac acaccgcaag gccggcaagc gcggcgatcg
     5641 tcgcgatcgg gccgagcgta tcggcggcct gagtaagaac ggtgagcggt gcgtgcgtgc
     5701 cggtgcgtgc ggcccacgtg tgccacagct cggcgaacac gccgcgctcc gcgcacgctg
     5761 caagtaccgc gatgccgatc gcgagcatgc cgacgatcgg gacgagcacg gtccaacgcg
     5821 ggcgcgacca cggccccgtg agcagccgga tgccggcgat cgcgagcaac gcgacgtacg
     5881 cgggatcgag gacgcccgcg agcagcgcgg cgccgatcgc ggggagcgga gacgcatcga
     5941 ccggcagcgg acgcgcgagc tcggcgatcg cgaacagggc cgcggcagcg gcgagcgtgg
     6001 cgcgcgggac atcgaagtgc ggcaggtcga gcacgccgag tgcgacgagc agcgcagcag
     6061 cggacgcggt agtcgcagcg gcgacggatg caccggcgaa cgcacgaacg gcggcggcag
     6121 cggcggcggc aagtgcaccg atcgcgactg cggcggatgg ctgctcgccc agcagggccg
     6181 ccaccgcggc gcacgcgatc gccatgatcg ggatcgcgag ccacgtcttt ggaggcacca
     6241 cagtccgacg ctacgcagcc cgacgtaacg gggcaaactt tcacccgttt ggctgatggc
     6301 gggcgaccca ccaggccagc acgagcccgg gaatcgccat cagcgcggtc gccaagaaga
     6361 agccggacca ggcgagcgag gtcacgacgt ggtcggcgag cggtccgaac acgcgctggc
     6421 caaccgatga cagcgacgtc aagagtgcca tctgggtcgc gctgaccgcg ggcgtgcacg
     6481 agcccatcag cacggacaga aacgcggtcg tgctgagtgc gtaggacagc tgatcgacga
     6541 acaccgctgc gcagaacacg ctgatgtcgt ggccgctcac cgcgagccag ctatagagga
     6601 ggttcgtgat cgccgacagg atgccgaacg tgacgagcat ccggcgcagg ccgaaccgtg
     6661 cgacaagcgc gccagcgaat gcgccgccga tcgcgagacc ggcaaagccg atgcctttga
     6721 acacaactcc gatctcggtg aacgagaact tcacgccgtc cttgaggaac ggaatgatga
     6781 gcgactgcgc gaacgagtcg ccgaaccggt acagcgcggc gaagccaata acgacgacga
     6841 ggccgcgcgt gcccaggcgc tgccagaatt cgcgaaacgg cagcacgagt gcgtcggcga
     6901 acgtacgcgg cggcgccacg cgttcgggtg gctcgtcggc gacgagcgtc gcgatcacgc
     6961 cgaggctcat gaggccggcc atgccgaagt agatcgtccg ccacggcagg tgatcggcag
     7021 cgatcaatgc gagggttccc gacattaaaa ctgcgacgcg atagccgagc acgtaggccg
     7081 ccgagccggc tgctcgctcc tgctccgcca gggtgtcggt catgtacgcg tcgatgatga
     7141 tgtcctgcga cgccgaacag aacgcgacgg cgatcgcgat ggcggcaagc tcgtacggat
     7201 gcgcacgcgg atcgctcagc gccatgatgg cggtcgtgac cacgagcaag agctggaacg
     7261 cgagcagcca gccacgccgg cggccgagaa acggcagctg atagcggtcg agcagcggcg
     7321 cccacagcac ttttagcgtg taggccaggc cgaatgcggc gacatcatcg atgcggccgc
     7381 gctcgacacc gaccgccgtc atccaggcct gcagggtctg gctggtcaga tacagcggca
     7441 ggccaccggc aaagccgagc aggaacagca cgaccattcg cttcgagcga aagaccgcga
     7501 agaagccgga cttcggagtt tcttgcagcg tcacgatcgt gccagcaggg gttccggtag
     7561 gtaagatcgc gcggaccgcg tggtgcattc cagtaaacga gcaaagctgg cggcagcagt
     7621 gcgctggctt gcaccgagcg cagcagcggc cgcgggcggc gccgcgcttg ccggtgcgct
     7681 cgaaggcgcc gggatgcacg ggctgttcgg gagcgccgcg gcggcagggt tcgtcgcgct
     7741 cgtcgcgttt ccggcgctgc tcgctgcgag tgccgcgacc cgcgggctgt ggcacgcgtg
     7801 gcagccgcgc gcgctcggca tgatcgatga cggcggtggt gcgccgcgcc tcgcgggttg
     7861 gttcgcattc gtgtggctcg cgacgctcgc gctcgcgtgg gtgatgttcc agggcacctg
     7921 gctgctcgcc ggcgcgaccg cgttcaagcc gaacggcatg gcgttcgccg agcccgtgct
     7981 cgcggtgatc accgtgctgc tgctcgtcgc gttgtcacgg ccgaccgcgc gcggtctgac
     8041 gtggatgtat cgcaagatcg atcagcgctt ccggatgacg ccgctgcgca tcatcgcgag
     8101 cctcgtcatc aagacggtga tcatcgcgta cctcgtgtgg cacttcttcg ccgcgaagcg
     8161 aatcggtgcg ctcgacttca gcatcctcca cgttccgctc gccggcgtga tggcgacggc
     8221 gatcgtccat gtggtctggg aacgtgcgac gcgtgtgcgg cgcatcgtcg gcggcgtgct
     8281 cggtgcggtc gcggcggtgt tgatcggttg cgcgctggtc gcatggcaga cgcgccctgc
     8341 cctcacgctg cagatctggg gcgagcgacc gatcgcgggc ctcgcgatcg acaagctctt
     8401 cgatctcgat tcgattcgcg ccggcgtctc gctcagcgag ttccggccga tcgagaaagc
     8461 cgggtcgccg catcccgaca tcatcttgat cacgatcgat acggtgcgcg ccgatcacac
     8521 gccgccgtac ggtggcaccg ccgagatgcc gctgctccgc gagcttgcgg tgcgcggtgc
     8581 ggtctacgac tgggcattcg cgccgagcaa cgtgacgcga cgctcgatcc cgtcgatggt
     8641 gacgggcctc gcgccgaatc gcgtgcgcgg ccgcgtcgtc ggctgggcgc tccgcatcga
     8701 tccgcgtcac gtcgtgctcg ccgagcgcat gctcgccggt ggctacgaga cggcaggctt
     8761 cgtgtgctgc tacggcttct gggggcacga cttccgcacc ggtctgcagc gcggcctctc
     8821 gcatctcgag atcgagccaa gcggcatcaa gctcgcgaag caagcgcggc agtggctcga
     8881 agcgcgcgag aagcagccga acaagaagcc gctgttcttg tggatgcaca tcctcgagcc
     8941 gcacaactgg caacagggcc aaggcacgcc gcaaaacgac gaggatcgcc gtcgcctcta
     9001 tgatcgatcg ctgactgcgg ccgacacgat gatggtcgag ctgttgacct cgctgtcggc
     9061 gcgcaagccc gaagacgcgc cgatcgtgat catcaccgcc gatcacggcg aggcactcgg
     9121 cgagcacggg cagccgtttc actcgaccga tctctacaac tcgcagatcc gcgtgccgtt
     9181 cgtcgtcgca ggtccgggca tcaagcccgg ccgcatctcc gagaccgtga gcctcaccga
     9241 cctgacgccg accgtgctcg agcttgccgg gttcgtaccg ccgaccggtc agagcatgga
     9301 cggccgctcg gtggccgatc tggcaaccgg ccggcggctc agcaccgagg acggcggcat
     9361 cgcgttctcg gcgatgatca gggatcgcag caaccccggc ggcatcacgt cgctgattcg
     9421 cggccgctgg aagatgatcg acagcgggaa ttcgctcgag ctctacgacg ttcatgccga
     9481 tccgaacgag acgtcgaacc tcgcgacaac gcgcccgcag gtctacaacg agctgcgtgg
     9541 cattctgcag cagtacgtga agcgcggcgg cgaatcgcca ttcgagtgat acgatcgagc
     9601 catgtcggtc gccgtctgcc cgatgtgcca ccggtcgaat cgaccgagcg cgaaagaatg
     9661 cgacgactgc ggctacgagt tcggccagag catcgaggct ctgcgcggca tgctccagaa
     9721 tcagctgctg aatacgcgga cgatgttctg ggtgctcgtc gcggctaacc tcgcgctcgc
     9781 ggcggtcgtc gcgatcggcg tgatgtacgg cttcatgatc ctgccgatgc tgccgttctt
     9841 gttcatcacg tatcagtcgg tgcgcgccgg ccagaagatc gcggtcacga agcacagcct
     9901 gcgcctgatc gagcagaagc agctgccgcg cgcgacgctc gtctcaggat agccgcgcga
     9961 aatcggtctg cacgagcaga tacgtcaggc gcagcaagtt gattcgcgcc tccggcgaac
    10021 gcacgcgcgc cgccagatcg ccgagtcgcg tgcggccatc gagcaagctg tacacgcgca
    10081 tgagcgcttc gccgagcccg aactgcgcga gctcgctttc ctgcgccggc tcgcgcgcga
    10141 cgaacgcacg ctgctgcgcc tgcagccatt cctcgacgag cggttcgccg agctgcgccg
    10201 cacctgcacc gatcacgcga aacgcgtcga gatgcagcga ccgcgccttg tacgggttgg
    10261 tctggcgcgg cgtccacgtg tagcggccct tctgccacga gaacgcctcc atcagcttcg
    10321 cggcgacctg cttcgcgagc aggcgatacg cctcgagcgg atcgagcaga tcgagaccga
    10381 cgagcgtgtc ggcgagacgg ccgccgaagt gatgcatgac ggacagcgcg cgctgcagtg
    10441 cttgtggcga cagcgccttc tgctcgagca agaagttacc aagccgctcg ctctcgacgt
    10501 tgctgttgac gaactgcggc tggccgtcga ggaagtacgc ttccttcagt acgctggcgc
    10561 ggccctcgag ggtgagcagg ccagtgccct tcgtgcgcgc gagcttgtac acgagacgca
    10621 acaccgactg ctcgcgcaag tcgccgacct cgcgcggtcc ggacgccgcc tcgcgcggct
    10681 gctccggcat gatgtcatct tcgctgatcg tcatcgtgcc gctcgagccc atgtcgatcg
    10741 tcggatcggg cacggccggc tcgggcacgc cgctgttggc gatgaactcg gcgcgcgcct
    10801 gctgcgcttc gagctcggct tgcgcctgat gcatgcgcgt catcgggccc gatagtcccg
    10861 acgggccttc gtcgacggcc gcttggcgag gttggccttc gagatccgcg gtcggcgagt
    10921 tgatcacgcg gccgatcagg ttcgcgagat cgcgctggcc aacccgcgtg gtgcggcgga
    10981 tccactcgtc gagcgcatcg cggaactgcg cagcggtctg ccagcgatcc tcggggcggc
    11041 gctgcaacgc gcgcaccgtg agcacgcgta gctcgaccgg gaactcgctc gcatacttgt
    11101 ggagacgatc gagacgcgcg tcgcgcacca tcagcaggat gtcgaggtca ttggtcgacg
    11161 tgaacagccg gcgcgccatc accatctcgg cgagcaggat gcccaccgag aa
//
LOCUS       DASUFX010000323         8781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1854973, whole genome shotgun sequence.
ACCESSION   DASUFX010000323 DASUFX010000000
VERSION     DASUFX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854973"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFV99_13000"
     CDS             <1..809
                     /locus_tag="VFV99_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5060276.1"
                     /translation="EGALDIIQKERVTCSLMVPTMLIRLSNLPKETLAKYDTSSLRWV
                     MSAAAPLTTEAARRFMAQFGPVLWNFYGATETGLVTLAGPHDHVSRPGTIGRRLRGNE
                     IRLLDDSGNDVRVGEVGELYARNPTLISGYHGNDDATRSSQRDGFFSVGDCGRTDADG
                     YYYLESRKSDMVISGGVNIYPREIEDHLSTHPAILEAAVIGVPDPEWGETLRAFVVLR
                     NGQQLSETEVINFCREHLADYKRPRKVTFLTEMPRNPTGKILKRELRDMQ"
     gene            806..1171
                     /locus_tag="VFV99_13005"
     CDS             806..1171
                     /locus_tag="VFV99_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5060277.1"
                     /translation="MRLGQLILFSGDSARLAAFYRDVLGLTVLEDEPGWVVLDAGGVK
                     LAIHQIPKEYAGEVSTPPKKREDSYWKPTFVVDDLDAVLAKLASHGVAMSPQRTFGPR
                     TFSDGMDVDGNVFQVARHR"
     gene            1168..3117
                     /gene="selB"
                     /locus_tag="VFV99_13010"
     CDS             1168..3117
                     /gene="selB"
                     /locus_tag="VFV99_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX5060278.1"
                     /translation="MIHPIVLGTAGHIDHGKTSLVRALTGIDTDRLPVEKARGITTEL
                     GFARLDLGDRRVAVVDVPGHERFVKSMVAGATGLDLVMLVIAADEGVMPQTREHLDIC
                     DLLGVRRGLIVLTKRDLVDDEWLGMVTGDVQTAVAGTFLEGAPIVPVSTKTPDTSTGL
                     DELRAALVKQIDALPPRAQTGVFRLPIDRVFTVKGFGTIVTGTVLGGEVKIGDELAVI
                     PTGLTTRVRGIEVHGAAVDKAVAGHRAALNLGNIAVEDLARGDLLAHPGRVAASHILD
                     VELRYLRSAPGPLPMRSKVLVHHGTTQVLASLVLVDPASASESDPLHSRAKELAPGST
                     GLAQLRLDQTTPLGALPDDHFIVRGFVASASGGSTIGGGRVIRVLAPKARKGASHAQA
                     VAALASARLDERVALDIKSSAFAGLGVADLVRRLGVPAESLRAPLAKLTAAGELLVTG
                     DDEHAHYLHAQAVADLEQRITKLVGAADIDGVAREPLRTQLPAALPARAYDAILAGLE
                     NRGLVVAAADRVKKSTAPKTAALSPVESAVLAKLEQTGIEPPRPKELPAALGLAEPQV
                     KTAIDRLIAAKLAIKVKPDLVMHARTVADVRTKLLAHLDQHGTIDAQQWKDLTGASRK
                     FTIPLAEYFDAEKLTLRVGDVRRRR"
     gene            complement(3126..3893)
                     /locus_tag="VFV99_13015"
     CDS             complement(3126..3893)
                     /locus_tag="VFV99_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060279.1"
                     /translation="MLTVLGAAGTAWGQALDDEGKPVPTTDPAAAPTSLVPPGNQIEY
                     GADIRLRQVFVPKGLIGLFVERVPGGASNTGFGVDLVRRRGNLELQLGFEFEHIEPKE
                     GVFINSGDNVAGGDEADYLISADRAGKSLGWFTLEFTFLNHAPINKYVAVRYGGGAGI
                     GFLTGGLQKYDVPCNGATNENPEPGCVPVGVTSPSGVQGTAGAARGPNDYDLPPVFPV
                     VNAIIGVQIRPTPKAVVNIETGIRTIPFIGLSAGYFF"
     gene            3989..5545
                     /locus_tag="VFV99_13020"
     CDS             3989..5545
                     /locus_tag="VFV99_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060280.1"
                     /translation="MRHGSLIGAVLFVLAGCPGGDAGLGERCGSHSDCAGQLQCFANI
                     CQPRCERAPDCGDGYRCDNDGVCIAATGQLGDTCKSEVECAAGLSCQIEGTELADDGF
                     LLASCVVENSSHPAGAECQTSGDCRNGTCDLGHCIDLCGSDRDCGTGTMCTQIPRLIG
                     TFSNQIQVQYRGCLQSQGAVRWSIPVTSANDEIELPVPSSARSVSMLFTVDDPNQKVG
                     ATAVTIPGVDTCGQTLCDVMTMYDYYANPFVRHRTEFGASVLSMPISPAPPAQLRTGV
                     YTMRVKSLRQTDPVTCQTPPCFGQGTATPAVTAVIKVDEAAILDLHFYFLNLDDHPCG
                     DAFGGKLDAATAQGASYFEQYLGEIKNIIGNAVYFEVGNVTYEDLRDHADLDGLDVDN
                     AASLLALGKHATGINVFFVRTLSPIGLQAVGPNPGPAALGGTPRSGIVIGVDTLCYRS
                     WTQLARLTTREIARYMGLYDNTGLDPMQNDPIADTDMSSVNLMFYSELGGTYLSDGQR
                     QILRRSAVLR"
     gene            5542..6129
                     /locus_tag="VFV99_13025"
     CDS             5542..6129
                     /locus_tag="VFV99_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5060281.1"
                     /translation="MRTILVTLFLTGAVAIATGTTNLDPDEQNTLTTIDTVPTKPQID
                     SVFGSTALQKLTAIANDPDPTADIGIRLRAIHALAKYCAATPCAANDVAHQSVASVVT
                     STATSHDGSQVLLLRAAIETLGAMRVTGDAPSLWPLLDHPSRDIRAATARALRDLCNN
                     QAVTPLRARYSVEMTDQVKLAISEALRILGQCSGM"
     gene            6344..8368
                     /locus_tag="VFV99_13030"
     CDS             6344..8368
                     /locus_tag="VFV99_13030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060282.1"
                     /translation="MQIDISNAPPDDERDSHPSLAMGTSDVSREDRTKIESPSGRNKT
                     PQPKPPTRPPPTPNRSADDPEIRIGQVLGAYKLAELLGKGGMGYVYRAEHVKLGREVA
                     LKLLRGDYAKRRDAVLRFFQEAKTVNRVRHRNIVDVTDFVELDDGTTFIIMEFLRGQS
                     LGKWARTGIDLPRALAVLVQICDGLGAAHAVGVVHRDLKPDNVIVVPTSDGAEMVKLL
                     DFGVAKLLNRDDEDLGFQTAAGSVIGTPAYMSPEQAGGMVVDHRSDIYSLGAIMYELF
                     CGQPMFKGRSFGEYVRKHLTEMPVPPRSTPNGGAIDPVLEALIMKCLAKDPNERFSHI
                     LELRDGLLHMFGGYETQPPTYASLAQSAIRPPPTAGMTAVGMSQLPPHILPVPAPAMV
                     TTVPTPPSSSQSHVSQLSGIASIPSLPSIYSQYSAQPPEPVLPPEPTTPWWVWFVGGA
                     FAVGLGIAGALWYANKSGGEDTPKPAPVVQPTANPIETDTASPKLVELRFDSLPSGGV
                     YADGRSAELCRTPCNFNVDLSDGGPKDRRTFVVRADGYQDSRVDVDFTKSERDFSVSL
                     AKNPTVPTVTQQPTVVDMSADEADETVETPHAEKGSTKKAGGKKGKKTEEKKASTTVE
                     QPAEEKKPEDKKPLVDDTKLEPIEKKPEEKKKPAGPIDPSDTIDPFRRKK"
     gene            8426..>8781
                     /locus_tag="VFV99_13035"
     CDS             8426..>8781
                     /locus_tag="VFV99_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060283.1"
                     /translation="MAVPARTANADDPATRAARRHYERGEKLFALGKFDDALEEYQTA
                     FDAKPLPGFLYNIGQCYRNLGDYDQAIFSFKKYLKLEPEASNKEAVERLVEELEEKKA
                     QGESEKFVRKPKKEPTP"
BASE COUNT         1573 a         3003 c         2787 g         1418 t
ORIGIN      
        1 ccgagggcgc gctcgacatc atccagaagg agcgcgtgac gtgctctctg atggtgccga
       61 cgatgttgat ccgcctgtcg aacctgccga aggagacgct cgcgaagtac gacacgtcga
      121 gcctgcgctg ggtgatgtcg gccgccgcgc cgctaaccac cgaggccgcg cgccgcttca
      181 tggcgcagtt cgggccggtg ctgtggaact tctacggagc gaccgagacc gggctcgtca
      241 cgctcgctgg gcctcacgat cacgtgtcgc ggccgggcac gatcggcaga cggctgcgtg
      301 gcaacgagat ccgattgctc gacgatagcg gtaatgacgt gcgcgtcggc gaggtcggcg
      361 agctttacgc gcgcaacccg acgctgatct ccggttatca cggcaacgac gacgcgaccc
      421 gctcctcgca gcgcgatggt ttcttctccg tcggcgactg cggccgcacc gatgccgatg
      481 gctactacta cctggagtcg cgcaagagcg acatggtgat ctccggcggc gtgaacatct
      541 atccgcgcga gatcgaagat cacctgagca cgcaccccgc gatcctcgag gcggcggtga
      601 tcggcgttcc ggatcccgag tggggcgaga cgctacgtgc gtttgtcgtg ctgcgtaacg
      661 gccagcagct cagcgagacc gaggtcatca acttctgccg cgagcacctc gccgattaca
      721 agcgcccgcg caaggtcacg ttcctcaccg agatgccgcg caaccccact ggcaagatcc
      781 tcaagcgcga gctacgagac atgcaatgag gctcggccag ctgatcttgt tctccggcga
      841 ttcggcgcgc ctcgcggcgt tctatcgcga cgtgctcggg cttaccgtgc tcgaggatga
      901 gccaggctgg gtggtgctcg acgctggcgg cgtgaaactg gcgatccatc agatcccgaa
      961 ggagtacgcc ggcgaggtct cgacgccacc gaagaagcgc gaggactcgt actggaaacc
     1021 gacgtttgtc gtcgacgatc tcgatgcggt gctcgcgaag ctcgccagcc acggcgtcgc
     1081 gatgtcgccg caacgcacgt tcggtccgcg cacgttctcc gacggcatgg acgtggacgg
     1141 caacgtgttc caagtcgcca ggcaccgatg atccatccga tcgtcctcgg caccgcaggc
     1201 cacatcgatc acggcaagac gtcgctcgtg cgcgcgctca ccggcatcga taccgatcga
     1261 ctgcccgtcg agaaagcgcg cggcatcacg acggagctcg gcttcgcgcg cctcgacctc
     1321 ggcgaccgtc gcgtcgcggt cgtcgacgtg cccggtcacg agcgcttcgt gaagagcatg
     1381 gtcgcgggcg cgaccggcct cgatctcgtc atgctcgtga ttgcggcaga cgaaggcgtg
     1441 atgccgcaga cgcgcgagca cctcgacatc tgcgacctac tcggcgtgcg ccgcggcttg
     1501 atcgtgctga cgaagcgcga cctcgtcgac gacgaatggc tcggcatggt cacaggcgat
     1561 gttcaaacgg cggtcgccgg cacgttcctc gagggcgcgc cgatcgtgcc ggtctcgacg
     1621 aagaccccgg ataccagcac tggtctcgac gagctccgcg ccgcgctcgt gaagcagatc
     1681 gatgcgctac cgcctcgtgc gcagaccggc gtgttccggc tgccgatcga tcgcgtgttc
     1741 acggtcaaag gcttcggtac gatcgtcacg ggcaccgtgc tcggcggcga agtaaagatc
     1801 ggcgacgagc tcgccgtgat tccgaccggc ctcacgacgc gcgtgcgcgg catcgaagtc
     1861 catggcgccg ccgtcgacaa ggcggtcgcc ggtcatcgcg ccgcgctcaa cctcggcaac
     1921 atcgcggtcg aggatctcgc gcgcggcgat ctgctcgcgc atcccggacg cgtcgcggcg
     1981 tcacacatcc tcgacgtcga gctgcgctac ctgcgctcgg cacctggccc gttgccgatg
     2041 cgcagcaagg tgctcgtcca tcacggcacc acgcaagtgc tcgcatcgct cgtgctcgtc
     2101 gatcccgcga gtgcgagtga atcggacccg ctccattctc gggccaagga gctcgcgccc
     2161 ggctcgacgg gcctcgcgca gctgcgtctc gatcaaacga caccactcgg tgcccttccc
     2221 gacgatcact tcatcgtgcg tggcttcgtc gcatcggcgt cgggcggctc gacgatcggt
     2281 ggcggacgcg tgatccgcgt gctcgctccg aaagcgcgca agggcgctag ccatgcccag
     2341 gccgtcgcag cactcgcgtc cgcacggctc gacgaacgcg tcgcgctcga catcaagtcg
     2401 tcagcattcg cgggccttgg cgtcgcggac ctggtccgcc ggctcggcgt gccggccgaa
     2461 tcgctgcgtg cgccactcgc gaagctcacc gccgccggtg aattgctcgt gaccggtgac
     2521 gacgagcacg cgcattacct ccacgcgcaa gccgtcgccg acctcgagca acgaatcacc
     2581 aagctcgtcg gcgcggccga tatcgacggt gtcgcgcgcg agccgttgcg cacccagctt
     2641 ccagccgcgc ttcccgcccg tgcgtacgac gcgatcctcg ccgggctcga gaaccgcggc
     2701 cttgtcgtcg ccgccgccga tcgggtgaag aagtcgaccg caccgaagac ggcggcgctc
     2761 tcgccggtcg agtcggccgt gctcgcgaag ctcgagcaaa ccggcatcga gccgccgcgc
     2821 cccaaagagt tacccgctgc tctcggcctc gccgagccgc aggtgaagac cgcgatcgat
     2881 cgtctgatcg ctgcgaagct cgcgatcaaa gtgaaaccgg atctcgtgat gcacgcccgc
     2941 accgtcgccg acgtacgcac gaagctgctc gcgcacctcg atcagcacgg cacgatcgac
     3001 gcgcagcagt ggaaggatct caccggcgcg tcgcgcaagt tcaccatccc actcgccgag
     3061 tacttcgacg ccgagaagct cacgctccgc gtcggcgacg tgcggcgccg gcgctagaag
     3121 ccaggctaga agaagtaacc cgccgacagg ccgatgaacg ggatcgtgcg aatgccggtc
     3181 tcgatgttga ccacggcctt cggcgtcgga cgaatctgca cgccgatgat cgcgttgacg
     3241 accgggaaca ccggcggcaa gtcgtaatcg ttcggaccgc gcgcggcacc cgccgtgccc
     3301 tgcacgccgc tcggtgaggt gacgccaacg ggcacgcagc ccggctccgg gttttcgttc
     3361 gtcgcgccgt tgcacggcac gtcgtacttc tggaggccac cggtcaaaaa gccaatcccc
     3421 gcgccgccac cgtagcgcac ggcgacgtac ttgttgatcg gcgcatggtt caagaacgtg
     3481 aactcgagcg tgaaccagcc aaggctcttg cccgctcgat ccgccgagat caggtagtcg
     3541 gcctcgtcgc cgccggcgac gttatcgccg ctgttgatga acacgccctc cttcggctcg
     3601 atgtgctcga actcgaagcc gagctgcagc tcgaggttgc cgcgtcgacg caccaggtcg
     3661 acgccgaaac ccgtattact cgcaccgccg ggcacgcgtt cgacgaacag gccaatcagc
     3721 cctttgggca cgaagacctg ccggagccga atatcggcgc cgtattcgat ctggtttccc
     3781 ggcgggacaa gcgacgtcgg cgcagccgcc ggatcggtcg tcgggacagg ttttccctcg
     3841 tcgtcgagcg cctgccccca ggcagtgccc gccgcaccaa ggaccgtaag caaagtcagg
     3901 ctcgtggcga aacgcatgat tcccccgcgc tgcgacaata tatacttcag gtcactgagt
     3961 cccgtaaaac cgtgcgggat tggcgttcat gcggcatggc tcactgatcg gcgcagtact
     4021 gttcgtcctc gcgggctgtc ccggcggcga tgcggggctc ggcgagcgct gtggcagcca
     4081 tagtgactgt gccggtcagc tgcaatgctt cgcgaacatc tgccagcctc gttgtgaacg
     4141 tgcgccggat tgtggtgacg gctatcgctg tgacaacgac ggcgtgtgca tcgccgcaac
     4201 cggccagctc ggcgacacct gcaagagcga ggtcgagtgc gccgcgggat tgtcgtgtca
     4261 aatcgagggc accgagctcg ccgacgacgg ctttctcctc gcgagctgcg tcgtcgagaa
     4321 ctcgagtcac ccggccggcg ccgagtgcca gacgagcggc gattgtcgca acggtacctg
     4381 cgatctcggc cattgcatcg acctgtgcgg cagcgatcgc gactgcggca cgggcacgat
     4441 gtgcacgcag attccccgcc tcatcggcac gttctccaac cagatccagg tgcagtaccg
     4501 cggctgcttg cagtcacaag gcgccgtgcg ctggtcgatt ccggtgacga gcgcaaacga
     4561 cgagatcgag ctgccggtgc cgtcgagcgc acgttcggta tcgatgctgt tcaccgtcga
     4621 cgatccgaac cagaaggtcg gagcgaccgc ggtcacgatc ccgggcgtcg acacgtgcgg
     4681 ccaaacgctg tgcgacgtga tgacgatgta cgactactac gcgaacccgt tcgtccgcca
     4741 ccgtacggag ttcggcgcgt cggtgctcag catgccgatc tctcctgcac cacccgcgca
     4801 gctgcggacc ggcgtgtaca cgatgcgggt gaagtctctg cgtcagacgg atccagtgac
     4861 ctgccagacg ccgccgtgct ttggccaggg caccgcgacg ccggccgtca ccgccgtgat
     4921 caaggtcgac gaagcagcca ttctcgatct gcacttctac ttcttgaacc tcgacgacca
     4981 tccgtgcggc gacgcgttcg gcggcaagct cgatgcggcc accgcgcagg gggcgtcgta
     5041 cttcgagcag tacctcggcg agatcaagaa catcatcggc aacgcggtgt acttcgaggt
     5101 tggcaacgtc acgtacgagg atctgcgcga ccacgccgat ctcgacgggc tcgacgtcga
     5161 caacgcggcc tcgttgctcg cgctcggtaa gcacgcgacc gggatcaacg tcttcttcgt
     5221 gcgcacgctg tcaccgatcg ggctgcaagc cgtcggaccg aatcccggcc ccgcggccct
     5281 cggcggaacg ccgcgctcgg gcatcgtgat cggtgtcgat acgctctgct atcgctcgtg
     5341 gacgcagctc gcacgactca cgacgcgcga gatcgcgcgc tacatgggtc tctacgacaa
     5401 caccgggctc gatccgatgc agaacgatcc gatcgccgac acggacatgt cgagcgtgaa
     5461 cctcatgttc tactccgagc tcggcggcac gtacctctcc gacggtcagc gccagatcct
     5521 gcgcaggagc gcggtgctgc gatgagaacg atcctcgtca cgctgttcct caccggcgcc
     5581 gtcgcgatcg cgaccggcac cacgaacctc gatcccgacg agcagaatac gctgacgacg
     5641 atcgacaccg tgccgacgaa gccgcagatc gactcggtgt tcgggtcgac cgcgctccag
     5701 aagctcaccg cgatcgcgaa cgatccggat ccgaccgcgg acatcggcat ccgcctgcgc
     5761 gcgatccacg cgctcgcgaa gtactgcgcc gcgacgccgt gcgcggccaa cgacgtggcc
     5821 caccagtcgg tggcatcggt tgtcacctcg accgcgacct cgcacgacgg ctcgcaagta
     5881 ttgctgctcc gcgcggcgat cgagacgctt ggcgcgatgc gcgtcaccgg cgacgcgccg
     5941 agcttgtggc cacttctcga ccatccgagc cgggacattc gggctgcgac cgcgcgggct
     6001 ttgcgcgacc tctgcaacaa ccaagcggtc acgcccctcc gggcccgcta ctccgtcgag
     6061 atgaccgacc aggtgaagct cgcgatatct gaggctctcc ggattctggg ccagtgttcc
     6121 ggtatgtgat gggagcacgt ggcctagcgc acaaccgctc ggaatcgttt ggttgctgcg
     6181 gccgggggga cgatcgtaca attcccgtta cctcgctaca cgtctgcgcc tgatcgcgca
     6241 ctcgtcgggg gtgaacatga cgactcgcga tccgcgctct cgcgttccgc tgcgacctga
     6301 ctctggtcgt agtggtgaaa gcgcgcgcga gccgacgcag tcgttgcaga tcgacatcag
     6361 caacgcgcca cctgacgacg agcgcgattc gcatccgtca cttgcgatgg gcacgagcga
     6421 cgtcagtcgc gaagatcgca cgaagatcga atcgccgagc ggccgcaaca agacgccgca
     6481 acccaagcca ccgacgcgcc cgccgccgac gccgaaccgc tctgccgatg atccggagat
     6541 ccggatcggc caggtcctcg gcgcctacaa gctcgccgag ctgctcggca aaggcggcat
     6601 ggggtacgtg taccgtgccg agcacgtcaa gctcggccgc gaggtcgcgc tcaagctatt
     6661 gcgcggtgac tacgcgaagc ggcgcgacgc cgtgctccgg ttcttccagg aagcgaagac
     6721 cgtcaaccgc gttcgccacc gcaacatcgt cgacgtcacc gacttcgtcg agctcgacga
     6781 cggcacgacg ttcatcatca tggagtttct gcgcggccag agcctcggca agtgggcgcg
     6841 caccggcatc gacctgccgc gcgcgctcgc cgtgctcgtg cagatctgcg atggcctggg
     6901 cgccgcccac gcggtcggcg tcgtgcaccg tgatctcaag cctgacaacg tcatcgtcgt
     6961 gccgacgtcc gatggtgcgg agatggtcaa gctcctcgac ttcggcgtcg cgaagctgct
     7021 caaccgcgac gacgaggatc tcggcttcca gactgccgcc ggctcggtga tcggcacgcc
     7081 cgcgtacatg tcgcccgagc aagcgggagg catggtcgtc gaccatcgct ccgatatcta
     7141 ttcgctcggc gcgatcatgt acgagctgtt ttgcggtcag ccgatgttca agggccgttc
     7201 gttcggcgag tacgtgcgca agcacctcac cgagatgccg gttccgccgc gctcgacgcc
     7261 gaacggcggc gcgatcgatc ccgtcctcga ggcgctgatc atgaagtgtc tcgcgaagga
     7321 tccgaacgag cgcttctcgc acatcctcga gctgcgcgac gggttgctcc acatgttcgg
     7381 cggctatgaa acccaaccgc cgacctacgc gtcgctcgcg cagagcgcga tccggccacc
     7441 gccaacggca ggcatgaccg cggtcggcat gtcgcaattg ccgccgcaca tcctgccggt
     7501 gcctgcgccc gcgatggtga cgacggtgcc gacgccgccg tcgagctcgc aatcgcacgt
     7561 ctcgcagttg tcgggcatcg cgagcattcc gtcgctgccg tcgatctaca gccagtacag
     7621 cgcacagccg ccagagccgg tgctgccgcc ggaaccgacg acgccgtggt gggtgtggtt
     7681 tgtcggcggc gcgttcgcgg tcggcctcgg catcgccggc gcgctttggt acgcgaacaa
     7741 gagcggcggt gaggacacgc ccaagccggc gccggtcgtg caacccactg cgaatccgat
     7801 cgagaccgac accgcgagcc cgaagctcgt cgagctccgc ttcgattcgc tgccgagcgg
     7861 tggcgtctac gcggacggtc gctctgcgga gctgtgccgc accccgtgca atttcaacgt
     7921 cgatctgtcc gacggtggcc cgaaagatcg gcgcacgttc gtcgtacgcg ccgacggcta
     7981 tcaggactcg cgcgtcgacg tcgacttcac gaaatccgag cgtgatttct cggtatcgct
     8041 cgcgaagaac ccgaccgtgc cgaccgtcac gcagcagccg acggtcgtcg acatgtcagc
     8101 cgacgaggcc gacgagactg tcgaaacgcc acacgccgaa aaggggagca ccaagaaggc
     8161 gggtggtaaa aagggcaaga agaccgaaga gaagaaggcc tccaccaccg tcgagcagcc
     8221 agccgaagag aagaaaccgg aagacaagaa gccgctcgtc gacgacacga agctcgagcc
     8281 catcgaaaag aaaccggagg agaagaaaaa acctgccggt cccatcgatc ccagcgatac
     8341 gatcgacccg ttccgccgta agaaataacg actctcgtac gcaatgatta ctcgcctatt
     8401 cttgctgttg gtgatgtgtg tggccgtggc tgttcctgca cggaccgcga acgctgatga
     8461 tccagccacg cgcgccgcgc gccggcacta cgagcgcggc gagaagctgt tcgcgctcgg
     8521 caagttcgac gacgcgctcg aggagtacca gaccgcgttc gacgccaagc cgctgcccgg
     8581 cttcctctac aacatcggcc agtgctaccg aaacctcggt gactacgacc aggcgatctt
     8641 cagcttcaag aagtacctga agctcgagcc cgaggcgtcg aacaaggagg ccgtcgagcg
     8701 gctcgtcgag gagctcgagg agaagaaggc ccaaggcgag agcgagaaat tcgtccgcaa
     8761 gcccaagaag gagcccacac c
//
LOCUS       DASUFX010000324         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1994759, whole genome shotgun sequence.
ACCESSION   DASUFX010000324 DASUFX010000000
VERSION     DASUFX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994759"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFV99_13040"
     CDS             <1..520
                     /locus_tag="VFV99_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060284.1"
                     /translation="EKINNPQRIGNLQLALASLDLDDSQEASAIGRAETVQKDAAERG
                     ATSLEAYASVVLAHAHLGQAETQKALSDLDHVKAEPQALRLRVQYKIVDGLTHHFLGD
                     DDYAKEKIEAVRAETEKQTCVGLLLETKLARSQILPAEEGKAELDAVVRDAKAANFGR
                     IVKLAEALAQPQ"
     gene            537..1463
                     /locus_tag="VFV99_13045"
     CDS             537..1463
                     /locus_tag="VFV99_13045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060285.1"
                     /translation="MPDCQLCAGSHPDNACPQSRTGQTIDKYVVGPLLGVGGIAAVYA
                     AEHPVLRTQIAVKVLHRRWAKDAELTQRFVREARETAALGHPAFVRVHDAGTTDDGCA
                     YIEMDRLEGRDLYSLRTQFGPLDPERLARIAIHVLDALAVLHARGTIHRDLKSANIYL
                     VPSQDGEQIKLLDLGFAKANDQLALTQKDQMLGTPFYISPEQYLDPTAVDARADLFSL
                     GIVMWEALVGDFPYQWENKRDLLSKVMHGKLERHPAARRPDVPDWLDAIVARVLAHDK
                     NERFESALAMKDALEKREPPKKPGLLGRIFGT"
     gene            1460..1999
                     /locus_tag="VFV99_13050"
     CDS             1460..1999
                     /locus_tag="VFV99_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060286.1"
                     /translation="MIRALALVALASSAAAAQPAPGAVQPVPEEEPPRERTPFDRGRF
                     ALSAGAGTTSVFDQRYFGIGVAAGYFVLDGVAIDLGTQVQWGDGPTILRTTPGLRYVA
                     QPLVGTWPLIPYVAGYYTHWFVGDAIADVDAVGTRGGLLYVSGSLVLGLGVGYEHLVS
                     ECTMDCSSVYPDLTISIAL"
BASE COUNT          452 a          856 c          903 g          408 t
ORIGIN      
        1 cgagaagatc aacaacccgc agcggatcgg caatctgcag ctcgcgctgg cgtcgctcga
       61 tctcgacgac agtcaggaag cgagcgcgat cggccgcgcc gagaccgtgc agaaagatgc
      121 ggccgaacgc ggtgcgacga gtctcgaagc gtatgcctcc gtcgtgctcg cgcacgcaca
      181 cctcggccag gccgagaccc agaaggcgct ctccgacctc gatcacgtga aggccgagcc
      241 gcaggcgctg cgcttgcgcg tgcaatacaa gatcgtcgac ggcctcaccc atcacttcct
      301 gggcgacgac gactacgcga aggagaagat cgaagcagtg cgcgccgaga ccgagaagca
      361 aacgtgcgtc ggcctgttgc tcgagacgaa gctcgcgcgc tcgcagatac tcccggccga
      421 ggagggcaaa gccgagctcg acgccgtcgt gcgcgacgcc aaggccgcaa acttcgggcg
      481 catcgtgaag ctcgccgaag ccctcgcgca gccgcagtga gttattctac ccggcgatgc
      541 cggactgcca gctgtgcgca ggaagccatc ccgacaacgc gtgtccgcag tcgcgcacgg
      601 gacagacgat cgacaagtac gtcgtagggc cgttgctcgg cgtcggcggc atcgcggccg
      661 tgtatgccgc ggagcacccg gtgttgcgca cgcagatcgc ggtcaaggtg ctgcatcggc
      721 ggtgggcgaa ggacgccgag ctcacgcagc ggttcgtgcg cgaggcgcgc gagaccgcgg
      781 cacttggcca tccggcgttc gtgcgcgtac acgatgccgg cacgaccgac gacggctgcg
      841 cgtacatcga gatggatcgg ctcgagggtc gcgacctgta cagcttgcgc acgcagttcg
      901 gcccgctcga tcccgagcgg ctcgcgcgca tcgcgattca cgtgctcgat gcgctcgccg
      961 tgctccacgc gcgcggcacg atccatcgcg acctcaagag cgcgaacatc tatctggtgc
     1021 cgtcgcagga tggcgagcag atcaagctgc tcgatctcgg gtttgcgaag gcgaacgacc
     1081 agctcgcgct cacgcaaaaa gatcagatgc tcggcacgcc gttctacatc agccccgagc
     1141 agtatctcga tccgacagcg gtcgatgcgc gcgccgatct cttctcgctc ggcatcgtga
     1201 tgtgggaagc gctcgtcggc gacttcccgt atcagtggga gaacaagcgc gatctgttgt
     1261 cgaaggtgat gcacggcaag ctcgagcgtc atcccgcggc gcgacgaccc gacgtgcccg
     1321 actggctcga tgcgatcgtg gcgcgcgtgc tcgcgcacga caagaacgag cgcttcgagt
     1381 cggcgctcgc gatgaaggac gcgctcgaga agcgcgagcc gccgaagaag cccggcttgc
     1441 tcgggcggat cttcggcaca tgatccgggc gctcgcgctc gttgcgctag catcatcggc
     1501 cgcagccgcg caaccggcgc caggtgccgt tcagcccgtc cccgaggaag aaccgccacg
     1561 cgagaggact ccctttgatc gcgggcgctt cgcactgtcg gcgggcgcgg ggacgacgag
     1621 cgtgttcgac cagcgctact tcggcatcgg tgtggcggcg ggttacttcg tgctcgatgg
     1681 ggtcgcgatc gatctcggca cgcaggtgca gtggggcgac ggccccacga ttctgcggac
     1741 cacgcccggt ttgcgctatg tcgcgcagcc gctcgtcggc acctggccgc tgatcccgta
     1801 cgtcgcgggt tactacacgc actggtttgt cggcgacgcg atcgcggacg tcgatgcggt
     1861 cggcacgcgc ggtggtctgc tttacgtatc gggctcgctc gtcctcggcc tcggcgtcgg
     1921 gtacgagcat ctcgtatcgg aatgcacgat ggactgttcg tcggtgtatc cggacctgac
     1981 catcagcatc gcgctttagt cggtacgtcg tctgcacgct gactgctcgg aggttgtatg
     2041 cccgagcgtc aaacagtaca gaaggcaaaa cgcgagctgc gtcatgggcg ctcgccgtcg
     2101 acggcggcgg gtgagttcgt tcgcgaagaa atccatcacg tccgtgaggg caagcacggg
     2161 gcgcggtcga caaagcaggc gatcgcgatc ggattgtcga aggcgcgacg tgccggcgtt
     2221 ccgcttcccc cgggcggttc gcgctcgaca cgtaagcgcg cgagccgcga tctggccacg
     2281 gggcagcacc ggcgcaaacc acgtgcacca tcacggacgc gcgcacgtgc cacagagcgt
     2341 gcgctgaagc gcgaaccgcg gcgcgcggca tcgcatcgct cgctcgagcg gcaggcacgt
     2401 cggagcgcga agcagcgtgg ccaggcggcg agggtgaggg cggcccgcaa ggctgttcgt
     2461 acgaaggggc ccgcggtgcg tcgcgcggcg gcacggaagg ccgcgcgtac gcgcgcgcgt
     2521 cacgcctaga tgcgggtgtt tcgcgatcgc cgcgatgccg gcgtgcagct cgcatcgacc
     2581 ttgcgtcgtt atgccgaagc gaacgacgtg atcgttctc
//
LOCUS       DASUFX010000325        12424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1995411, whole genome shotgun sequence.
ACCESSION   DASUFX010000325 DASUFX010000000
VERSION     DASUFX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995411"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..765)
                     /locus_tag="VFV99_13055"
     CDS             complement(46..765)
                     /locus_tag="VFV99_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060287.1"
                     /translation="MARADEPIADNSFLVEEAYNQESGVVQHIQTMQLDLKSHDWGYT
                     FTEEWPAPNEEHQVSVTVPVNHTDGKTTLGDLYVHYRRELFHGDHVVVTPRASLWLRT
                     GQEAAPASGVQFQACVSVQPDGVLVLHSNVGVSAPLSNGSASDTYVVLGQSLIWLAQP
                     KLNLLVEAAATFPTATMSTDVSRELVVSPGLRTAIDFSSGLQIVPGVAAPLSIDADHH
                     AWALLFYLSFEHPLQSSRKDG"
     gene            complement(816..1403)
                     /locus_tag="VFV99_13060"
     CDS             complement(816..1403)
                     /locus_tag="VFV99_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5060288.1"
                     /translation="MTQSHVLDSQHLSFFIGAEQYGLGILEAREILQYPAVTAVPSRS
                     RAIRGVINLRGTAVPVVDLAAVFGHGVQPITSRTCVVVVASQSPNGESTLIGLVVERV
                     HQVFELRSENIERVPELGMRSRAKFFRGLNRTNDGFVLLLDLPRVLEHVDFDEDLLDI
                     GVDEPPLAEAEPAACHVSVGGDPDPQPETEESSQP"
     gene            complement(1400..3058)
                     /locus_tag="VFV99_13065"
     CDS             complement(1400..3058)
                     /locus_tag="VFV99_13065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX5060289.1"
                     /translation="MNDDVEVTREDILQVFAVEAKEQLDLLEDGLLKLEQDPGDPEVL
                     AELFRAAHSLKGNASVVDLTSIEAFTHTLEDVFDRIRSGSITPTSDLVSLLLRVVDAF
                     RRIIPKALAGIDDPHPEHAELLRWLASPARDSSAVAESTSDLSQSTVDDGSRNRTLRV
                     DAHKLDRMLDLLGEITIARSRLSSLLGLADVRSLPVLRDARDAHDDAEELYRELQSEV
                     LRSRLVRLGPTFRRYQRVVRDLALASGKHAHLVIDGEDAEVDLAVVEAIRDPLTHMIR
                     NAVDHGIEPPALRAAKGKPEIGTIVVRATREGSSVAISVADDGAGLDREQVLARARAM
                     KLVAADEVPSDSNLFKLIFEPGFSTAKIVSEVSGRGIGMSVVQRNIEALRGTVLLSSH
                     PGRGTTVTLRLPLTLAIIEGFSVGVGTETYVLPMDAVLACVEMPEAERTTDDAQGLID
                     VWGRPIPYVRLANLLGVERSPANRESVVILQHDGDRAGVVVDDLCGASQTVIKPLADL
                     VDRIPAVAGCTLLGSGRVALILDVPALFEAAASGTSSAQQVSEP"
     gene            complement(3058..3516)
                     /locus_tag="VFV99_13070"
     CDS             complement(3058..3516)
                     /locus_tag="VFV99_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX5060290.1"
                     /translation="MLNQVYLTPGSLYAATAPCVITTLVGSCVAVTMWSARLKVGGMN
                     HYLLPRQASGTERSPRYGETALAMLLARMEVLGCVAAEIETRIYGGAAVLATLSAGIS
                     LGEQNVVAAWQFARARALHVVDEQAGGKVARRLMLDVQTGAVDVKTLGGG"
     gene            complement(3510..4235)
                     /locus_tag="VFV99_13075"
     CDS             complement(3510..4235)
                     /locus_tag="VFV99_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5060291.1"
                     /translation="MSGNGNKPKHGYVARVRDNTRRYIEELLQENVRLRGVIDTVGRE
                     QQVLRRQLDDLRIDLDRREQQHARLLALLGAAESAARGFEDRFAEVEQQNANLARLYA
                     ASYQLHATMHRSEVLQAIQEIVINLIGSEELAILAGGPTLTPLASMGVDEERLGALDT
                     SAGALGRSLATRAPVLCDQDVAGIKASIPLVVGDRTLAVILIFGLLPQKPALDAFDRE
                     LLDLVATHAATALYCAELHEGKC"
     gene            complement(4238..4606)
                     /locus_tag="VFV99_13080"
     CDS             complement(4238..4606)
                     /locus_tag="VFV99_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060292.1"
                     /translation="MAKKILLVDDSNTVLLMERMILSHGRYELCTARNGREGVERARE
                     ERPDLILLDVVMPEMNGLDALAEIRKDATTRDIPVIMVTTRSEASSMETGFARGCNDY
                     VTKPVHSSELLTKVRNLLGE"
     gene            complement(4618..6084)
                     /locus_tag="VFV99_13085"
     CDS             complement(4618..6084)
                     /locus_tag="VFV99_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5060293.1"
                     /translation="MSLIGNLADVAVVDLMQFVHLGARTGTLVLERDNERAHISFQRG
                     RIVGAYGPTSPSVCELLVLKGDLLQSELDAVLAEHAKASPKVPLGQLLVAKGAVSADA
                     LREAITHKVERTVFDLIGWTFGTFRFELDEVRGDQEIALAPGDVIPQLDINTEMVLIE
                     ALRLFDERNRSGEIAPALPPPPAVAAPTRAPLTVQLVTEDIELVARVGAHLAPGISVR
                     TVELRDAGAPAPGEPPPLVLVDARRNVKPAAIDRVRKRHPRSLVVAIIAPATDPRELY
                     AIGVAAVVPADGAAIAACCVTLFGARGTTNDGAPTETRSSIARLRRVIADLRGGLVSA
                     TLSLNLMTIVADSVDRAILFLVQRDMMIALGAFGQHGNGQPLVESTQGMCVPIGSSGA
                     LSSCVAEGRACTVEYDGPDFPTDLRHIIDRPRSGQAVLFPVLGSRRVIALIYADNGKH
                     LRSVDDVEIVEIATAQVGLAFENELLRRQLDRSAYAAH"
     gene            complement(6081..6860)
                     /locus_tag="VFV99_13090"
     CDS             complement(6081..6860)
                     /locus_tag="VFV99_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5060294.1"
                     /translation="MAKAPTQVSERIDTELRHTVCNSSAIVMANGDVDAEQLLEVLRT
                     EFRRLNQVRSDRVAELDARIGTLEQDLRATEEMLIAAERQSARLASLYAAAYQLHSLD
                     PSEITAALSEIAVDLLGAHRCLLLMTSMERPGYDVVELVPGSIGRAPFASAQYDGGEP
                     IVDACLGDGRMRLGPDATAPTCAAVPLMLQGDVVGALVVLELLPQKPSLQREDRELLD
                     LLAAHAASALVGARVFQDTQRKLRTLEGLVALLRRSPGGAR"
     gene            complement(6899..8002)
                     /locus_tag="VFV99_13095"
     CDS             complement(6899..8002)
                     /locus_tag="VFV99_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060295.1"
                     /translation="MEMRAEAKDDWTFEEPTVAEEEREASARQNMAWAVAEQVRASAP
                     VNAVLVVARGTGMPTAVLVDDTRRHSRELDNVVLAEILAAAGDDQMVLALARLFLGDC
                     EDVEHTVARDRDGAITGVIAVRISGRVSSSWLRTVLARAANSLTGWLAVDVTWPPQSL
                     LEAIDEPALAHEGGIVLVANQPLARMLGREPNEVIGMPVSRITRRLAVHRACSLVVGG
                     RARPALLFDPPHAPPAGTSLLGCLERVLADRYTLLRRTTRVSIERRDQAEVAVPAPAL
                     EALVSIALLDATAMFVIPAQANHVKCWIYREDTMWVVLELIATGSLFHRPDTEHLGAV
                     VCATRTRELGGQFLSDTSRHDARVIRIKLPVAS"
     gene            complement(8106..8552)
                     /locus_tag="VFV99_13100"
     CDS             complement(8106..8552)
                     /locus_tag="VFV99_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060296.1"
                     /translation="MATTKDHISRETAAASRPRAISWRTVASHRQSPEQMYAAWPLLD
                     RRHMCHEAANYTFRPLRDIARDDLRRVLELLQVDRPGAARAVALKLRCLADDALLARH
                     AAIRSEALCAAAAADRWHVGIGADEARVDVLRTVIAIARQLRLTLL"
     gene            8545..10062
                     /locus_tag="VFV99_13105"
     CDS             8545..10062
                     /locus_tag="VFV99_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5060297.1"
                     /translation="MAIGSKPTWTDEQARAVLRVLEAVSRHRDREQLFVAIAGVLRSV
                     FKFDALIVNLDGPGPDQMTPYFIHPRVAIPALQRSRSALERVFLTQEPIYIRSRADVA
                     DRPGSLEAMEKFGAHSYVGLPLVIGGKVLAALLLQSNRPNAYDDLDLPFATEIASAIA
                     VALDNCLAYERIVQSRARLDAENQLLRAELRAAHLAGQMVAESSAMTEVKRLVELVAP
                     TDATVLITGETGAGKERLARLIHERSARADRPMLAINCAAIPSSLIESELFGHEAGAF
                     TGATRRRRGRFELANRGTLLLDEVGELAPDAQAKLLRVLQTQELERVGGSETLRVDVR
                     VIAATNRPLTEMVEAREFRPDLYYRLAVFPIELPPLWARRDDVPILARQLVAESAQRL
                     GIAPPHLDEQTIRELQRYSWPGNVRELQNVIERAVILSRGGLLDVAPLLDLSGLDAAD
                     EESDQDESTEGTEVRAALEASKWVIEGDDGAANRLGLRPSTLRSRMRRLGIERPR"
     gene            10059..11543
                     /locus_tag="VFV99_13110"
     CDS             10059..11543
                     /locus_tag="VFV99_13110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5060298.1"
                     /translation="MKLHSVLEVLRAIAGHRERRELMATLLQTLRASIRADYYLAAVK
                     RGRGSNEAEAFFDPPLTGVSTRVSDYSLVGDLLRGRAALFYEHDIGNHPVQRALWERY
                     GVRVSLRIPLMVEHEAVGTIALWSRDRHAFDGLDMDVMTELGMVVAVTIDSCLAYEHL
                     ARLRDEADDDNSYLREALADELQISEGIIGGGPAFATVKRQIEVAAPTETTVLLTGES
                     GTGKDVVARAIHAASPRRDRPLVTVNCAAIPNNLAETELFGHEEGAFTGAAKTRVGRF
                     EQANGGTLFLDEVGELPMAVQAKLLRVIQERELERVGGGDAIKIDVRIIAATNRNLQE
                     MINEGRFREDLFFRLAVFPIHLPPLRSRLEDLPELVEHFVKLAAERFRVPVRRVTPDV
                     VRRLADHDWPGNVRELQHAIERAMIVSPSDALAIDAVVPRRIAPPVSSGAGSDTLRAE
                     YLAALESAEWVIEGDDGAAARLGVHPNTLRHRLKRMGIARPTAR"
     gene            11579..12106
                     /locus_tag="VFV99_13115"
     CDS             11579..12106
                     /locus_tag="VFV99_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060299.1"
                     /translation="MSPVDVLGVVIKIPPEAVVPLLVGAGALLILMFFGAALADWKTK
                     HPETASKVGMVIAVALTGYALYMGATIIPAKYIDHGAPGETTIGFLDGPIMGFSALIG
                     ALFWRFANQRWLALGVGLGLGLAMFMKPFILPLHGWYDGHEHPWHFFDPEHLSFHGPG
                     VAVMIAGLIAGSRPK"
BASE COUNT         2094 a         4029 c         4157 g         2144 t
ORIGIN      
        1 aagaacgaga gaatcagctt gggaccaatc ttgagacctg ccatatcaac catccttccg
       61 agagctttgg agcgggtgtt cgaagctgag gtagaagagc aatgcccacg cgtgatggtc
      121 ggcatcgatg ctcaagggcg ccgcaacgcc cgggacgatc tgaagccctg acgagaaatc
      181 gatcgcggtg cgaaggcccg ggctcacgac gagctcgcgg gacacgtcgg tgctcatcgt
      241 cgctgtcggg aacgtcgccg ctgcctcgac gagcaggttg agcttgggtt gcgcgagcca
      301 gatcaagctc tgtccgagca cgacgtacgt atcgctcgcg ctgccattcg aaagtggcgc
      361 gctgaccccg acgttcgaat gcagcaccag cacgccgtcg ggttgaacgc tgacgcatgc
      421 ctgaaactga acgccggatg ctggggcagc ttcctgaccg gttcgcagcc acagactcgc
      481 gcgcggtgtc acgacgacgt ggtcgccgtg gaagagctcg cgccggtaat ggacgtagag
      541 gtcaccgagt gtggtcttgc cgtccgtgtg gttcacggga accgtgacgc tcacctggtg
      601 ttcttcgttg ggcgcgggcc actcttcggt gaacgtgtag ccccagtcgt ggctcttcag
      661 gtcgagctgc atcgtctgga tgtgctgcac gaccccggac tcttggttgt aggcttcttc
      721 gacgaggaag ctgttgtcgg cgatgggctc gtccgctcgg gccaccgaag acaccagtag
      781 cggcgcagga acgagccagg atagaagctt ggtggtcacg gctgagagga ctcctcggtt
      841 tctggttgcg gatctggatc gccgccgacc gacacgtggc acgcggccgg ctctgcttct
      901 gcgagcggcg gttcgtcgac gccgatgtct agaaggtctt cgtcgaaatc aacgtgctcg
      961 agcacgcgcg gcaggtcgag caggagaacg aacccgtcgt tcgtcctgtt gagaccgcgg
     1021 aagaatttcg cgcggctccg catgccgagc tccggcacgc gctcgatgtt ctcgctacgc
     1081 agctcgaaca cctgatgaac gcgctcgacg acgagaccga tgagtgtcga ctcgccgttc
     1141 ggggactgac tcgcaacgac cacgacgcac gttcggctgg tgatcggctg gacgccatga
     1201 ccgaacacgg ccgcgagatc gacaaccggc actgccgtgc cgcgaaggtt gatgacccca
     1261 cggatggcgc gcgaacgcga cggcacagcg gtgaccgccg ggtactggag gatctctcgt
     1321 gcctcgagga tgcccagtcc gtactgctcc gctccgatga agaagctgag gtgttgcgag
     1381 tcgagaacgt gtgattgcgt catggctcac tcacttgctg cgccgagctc gtgccactcg
     1441 cggcggcctc gaacagcgcg ggtacgtcca agatcagcgc gacgcgacca ctgccgagca
     1501 gcgtgcagcc cgcgacggct ggaatccgat cgacgaggtc cgcgagcggt ttgatgacgg
     1561 tctggctcgc gccgcagaga tcatcgacca ccacaccggc acggtcaccg tcgtgctgca
     1621 ggatgaccac gctctcgcgg ttcgcaggcg agcgctcgac gccgagtagg ttcgcgagtc
     1681 gcacgtacgg aatcggacgc ccccacacgt cgatgagccc ttgcgcatcg tcggtcgtgc
     1741 gttcggcctc aggcatctcg acgcacgcga gcacggcgtc catcgggagc acgtaggtct
     1801 cggtgccgac gccgacggag aagccctcga tgatcgcgag cgtcaacggc aggcgcagcg
     1861 tgacggtggt gccgcggcca gggtgactcg acaacaacac tgtgccgcgc agcgcttcga
     1921 tgttccgctg gacgacgctc atgccgatgc cgcgcccgga gacctcgctc actatcttcg
     1981 ccgtcgagaa accgggctcg aagatcagct tgaacagatt cgagtcgctc ggaacctcgt
     2041 cggctgcgac gagcttcata gcgcgtgcgc gcgcaaggac ctgttcgcgg tcgagcccgg
     2101 caccatcgtc agctaccgag atcgcgacgc tgctgccttc gcgggtcgcg cgtacgacga
     2161 tcgtgccgat ctccggtttg ccctttgcgg cgcgcaacgc gggcggctcg atcccgtgat
     2221 cgaccgcgtt gcggatcata tgagtgagcg ggtccctgat tgcttcgacg accgcgagat
     2281 ccacctcggc gtcctcgccg tcgatcacca ggtgcgcgtg cttgccgctc gcgagcgcga
     2341 ggtcgcggac gactcgctga tagcggcgga acgtcggtcc aagccggacc agacgcgagc
     2401 gcaacacctc actctggagc tcgcgataca gctcctctgc gtcgtcgtgc gcatcgcgcg
     2461 cgtcgcgcaa gacgggcagc gaccgaacat cggccaagcc gagcaggctg ctcaatcgac
     2521 tgcgcgcgat cgtgatctcg ccgagcaggt cgagcatccg atcgagcttg tgcgcgtcga
     2581 cgcgcagcgt acggtttcgc gaaccgtcgt cgacggtgga ttgcgagaga tcgctcgtgc
     2641 tctctgcgac agccgacgag tcacgggccg gtgacgccaa ccagcgaagt agctcggcgt
     2701 gctcggggtg cggatcatcg attcccgcga gcgccttcgg aatgatgcgg cgaaacgcat
     2761 cgacgacgcg caatagcagc gacacgagat cggaggtggg cgtgatcgag ccggaccgga
     2821 tgcgatcgaa gacgtcctcc agcgtgtgcg tgaacgcctc gatcgacgtg aggtcgacga
     2881 cgctggcatt gcctttgagc gaatgtgccg cgcggaacag ctcggcgagt acttcgggat
     2941 cgccgggatc ctgctcgagc ttcaagaggc cgtcttcgag caggtcgagt tgctctttgg
     3001 cctccacagc gaacacctga agaatgtctt cgcgcgtgac ttcgacatcg tcgttcatta
     3061 gccacctccg agcgtcttga cgtcgaccgc gccggtctga acgtcgagca tgagcctgcg
     3121 cgcgaccttg ccgccggctt gctcgtcgac gacgtggagc gcgcgtgcac gggcgaactg
     3181 ccatgccgcg acgacgtttt gttcgccgag cgagatgccg gccgagagcg tcgccagaac
     3241 tgctgcgccg ccgtagatgc gggtttcgat ctcggcagct acacagccga gcacttccat
     3301 gcgtgcgagc agcatcgcga gcgcggtctc gccgtaacgc ggcgagcgct cggtgccgct
     3361 cgcttgacgc ggcagcaagt agtgattcat gccgccgacc ttcaggcgcg ccgaccacat
     3421 cgtgaccgcg acgcacgaac cgacgagggt cgtgatcacg cacggtgcgg ttgccgcgta
     3481 gagcgaaccg ggcgtcagat agacttggtt cagcacttac cctcgtgcag ctccgcgcaa
     3541 tacagcgcgg tcgcagcgtg tgttgcgacg agatcgagaa gctcgcgatc gaacgcgtcg
     3601 agcgccggct tctgcggcag caggccgaaa atcaggatca cggcgagcgt gcgatcgccg
     3661 acaacgagtg ggatcgacgc cttgatgccg gcgacgtcct ggtcgcacaa caccggcgcc
     3721 cgagtcgcga gcgagcgtcc gagtgcgccg gcgctggtgt cgagtgcgcc gaggcgctct
     3781 tcgtcgacac ccatgctcgc gagcggcgtg agcgttggac cgccggccag gatcgcgagc
     3841 tcttcggacc cgatcaggtt gatgacgatc tcctggatcg cctgcaacac ctcgctgcga
     3901 tgcatcgtcg cgtgcagctg gtagctggcg gcatagaggc gcgccaggtt cgcgttctgc
     3961 tgttcgacct ccgcgaagcg gtcttcgaag ccgcgtgccg cagactcggc ggcgccgagc
     4021 aatgcgagca atcgcgcgtg ctgctgctcg cgacgatcga gatcgatccg cagatcatcg
     4081 agctgccgcc gcagcacctg ctgctcgcga ccaaccgtgt cgatcacacc gcgcaggcgc
     4141 acgttctctt gcagcagctc ttcgatgtag cgccgcgtgt tgtcgcgcac gcgcgcgacg
     4201 tacccatgct tgggtttgtt gccgttgccg ctcatcgtca ctctccgagg aggttgcgga
     4261 ccttggtgag cagctcgctg ctgtgcacgg gcttcgtgac gtagtcgttg cacccgcgcg
     4321 cgaaaccggt ctccatgctc gaggcttcgc tacgcgtcgt caccatgatc accgggatgt
     4381 cgcgggtggt cgcgtccttg cggatctctg cgagtgcgtc gagcccgttc atctcgggca
     4441 tcacgacgtc gagcaggatc agatcgggac gctcttcacg cgcgcgctcg acgccttcgc
     4501 ggccattgcg cgccgtgcac agctcgtagc ggccgtgcga caggatcatg cgctccatga
     4561 ggagcacggt gtttgaatcg tcgacgagta ggatcttctt ggccatgaca ctctccctca
     4621 gtgagctgcg tacgcgctgc gatcgagttg gcggcgcagc agctcgttct cgaatgcgag
     4681 tccaacttgt gcggtcgcga tctcgacgat ctcgacgtcg tcgaccgacc gcaggtgctt
     4741 gccgttgtcg gcgtagatga gcgcgatcac ccgccggctc ccgagcactg gaaacagcac
     4801 ggcttggccc gagcgcgggc gatcgatgat gtggcgtagg tcggtcggaa agtcaggccc
     4861 gtcgtactcg acggtgcagg cgcggccctc cgcgacgcag ctcgagagtg cgccactgct
     4921 gccgatcgga acgcacatgc cctgggtcga ttcgacaagc ggttgaccgt tgccgtgctg
     4981 accgaatgcg ccgagcgcga tcatcatgtc gcgctggacg aggaacagga tcgcgcgatc
     5041 gaccgagtcg gcgacgatcg tcatcaggtt cagcgacagc gttgcggaca cgaggccgcc
     5101 gcgcaggtcg gcgatcacgc ggcggagccg ggcgatgctg ctgcgcgtct cggtcggtgc
     5161 gccgtcgttt gttgtgccgc gcgcgccgaa cagggtcacg cagcacgcgg cgatcgccgc
     5221 gccgtccgcg ggaaccactg cagcaacacc gatcgcgtac agctcacgcg gatccgttgc
     5281 cggcgcgatg atcgcgacga cgagagaacg cgggtgacgc ttgcgcacgc gatcgatcgc
     5341 ggcaggcttg acgttccgtc gcgcgtccac cagcacgagc ggcggcggct caccgggagc
     5401 cggcgcaccg gcatcgcgaa gctcgacggt acgaacggag attccgggcg caaggtgcgc
     5461 gccgacgcgg gcgaccagct cgatatcttc cgtgacgagc tggactgtga ggggtgcccg
     5521 cgtcggcgca gcgacggctg gtggtggtgg aagcgcaggc gcgatttcgc ctgagcggtt
     5581 gcgctcgtcg aacaagcgga gcgcttcgat cagaaccatc tcggtgttga tgtcgagctg
     5641 ggggatgaca tcgccgggcg cgagcgcgat ctcttggtcg ccgcgcactt cgtcgagctc
     5701 gaagcggaac gtgccgaacg tccagccgat cagatcgaag accgtgcgct cgaccttgtg
     5761 cgtgatcgct tcgcgtagcg cgtcggcgct caccgcgccc tttgcgacga gaagctgacc
     5821 gagcggcacc ttcggcgatg ctttcgcgtg ctcggcgagc acggcgtcga gctcgctctg
     5881 cagtagatcg cccttgagca cgagcagctc gcacacgctg ggagacgtcg gtccatacgc
     5941 gccgacgatg cggccgcgtt gaaacgagat atgagcccgc tcgttgtcgc gttcgagcac
     6001 cagcgtgccg gtgcgggcgc cgaggtggac gaactgcatc agatcgacca ccgcgacatc
     6061 agcgagattg ccgatcagac tcatcgtgca cctccggggc tgcggcgcag cagcgcgacg
     6121 agaccttcga gggtgcgcag cttgcgttgg gtatcctgga acacgcgcgc accgacgagc
     6181 gccgatgcag cgtgagccgc aagtagatcg agaagctcgc ggtcctctcg ttgcagtgac
     6241 ggcttctgcg gcagtagctc gagcacgacg agcgcgccga cgacgtcgcc ctgcagcatc
     6301 agcggaaccg cggcacaggt cggcgcggtt gcatcgggac ccagccgcat tcgaccgtcg
     6361 ccgaggcacg cgtcgacgat cggttcgccg ccgtcgtact gcgcggatgc gaacggcgcg
     6421 cgcccgatcg agccgggtac gagctcgacg acgtcgtagc cgggccgctc catgctcgtc
     6481 atcagcagca ggcagcggtg tgcgccgagt agatcgactg cgatctccga gagcgcagcc
     6541 gtgatctccg atggatcgag cgagtgcagc tgatacgcag ccgcgtagag gctcgcgagc
     6601 cgcgccgact ggcgctccgc agcgatcaac atctcttcgg tagcgcggag atcctgctcg
     6661 agcgttccga tgcgtgcgtc gagctcggcg acgcgatcgc ttctcacttg attgagacgg
     6721 cggaactcgg tccgcaacac ctcgagcaac tgctccgcgt cgacgtcccc gttcgccatc
     6781 acgattgccg atgaattgca gacggtgtgc cgcagctcgg tgtcgatgcg ttcgctgact
     6841 tgcgtcgggg ccttcgccac gaagccgtgg tggccacggc ggctacggtc gtgagcgatc
     6901 acgacgccac cggcagcttg atacggatga cgcgagcgtc gtgtcgcgac gtgtcgctca
     6961 agaactgacc accaagctca cgcgtcctcg tcgcacacac gacggcaccg aggtgctcgg
     7021 tatcgggacg gtggaacagc gagcccgtcg cgatgagctc gaggacaacc cacatcgtgt
     7081 cctcgcggta gatccagcac ttcacgtggt tcgcctgtgc agggatcacg aacatcgcgg
     7141 tcgcgtcgag cagcgcgatc gagacgagcg cctcgagagc aggtgcgggg actgcgacct
     7201 ccgcttgatc ccgccgctcg atcgatacgc gcgtcgtacg gcgcagcagc gtgtagcgat
     7261 cggcgagcac gcgctcgagg cacccgagca gggacgtgcc tgcgggcgga gcatgcggag
     7321 ggtcgaacag cagcgcgggg cgtgcgcggc cgccgacgac cagcgagcac gcgcggtgaa
     7381 ctgccaatcg gcgcgtgatg cgtgataccg gcatgccgat cacttcgttc ggttcgcggc
     7441 cgagcatgcg agcgagcggt tggttggcga ccaggacaat gccgccctcg tgtgcgagcg
     7501 ccggttcgtc gatcgcttcg agaagagact gcggtggcca cgtcacgtcg accgcgagcc
     7561 agcccgtcag gctgtttgca gcgcgcgcga ggaccgtgcg cagccacgag ctcgaaacac
     7621 ggcccgagat gcggaccgcg atcacgccgg tgattgcacc gtcccgatct cgcgcgacgg
     7681 tgtgctcgac atcctcgcag tcgcccaaga acaggcgcgc gagcgccaac accatctgat
     7741 cgtcacctgc ggcggcgagg atctcggcga gcacgacgtt atcgagctcg cggctgtggc
     7801 gacgcgtgtc atccactagc acggccgtcg gcatgccggt gccccgtgcg actacgagca
     7861 cggcgttgac tggcgcggag gctcgcacct gctcggcgac cgcccacgcc atgttctgcc
     7921 gggccgacgc ctcgcgttcc tcctcagcaa cagtcggttc ctcgaacgtc caatcgtctt
     7981 tcgcttctgc cctcatctcc atggcggggg ccccatcgca actgccatgc ccgcgctaag
     8041 tggccgattc gagtcccgcc aagcgccacg acaaccccac gactcaccca ccaggtcgtc
     8101 gtgggtcaga gcagcgtgag tcgtagctgc cgggcgatcg cgatcacggt gcggagcaca
     8161 tcgacgcgtg cttcgtcagc accgattccg acatgccacc ggtccgctgc agccgcggcg
     8221 cacaacgcct cggaacgaat cgccgcgtgc cgcgcgagca gcgcatcatc cgccaggcac
     8281 ctcaatttca gtgctactgc acgagcagct cccggccgat cgacctggag aagctcgagc
     8341 acacgccgga gatcgtcacg cgcgatgtcg cggagcggac gaaaggtgta gttcgcagcc
     8401 tcgtgacaca tatgacgccg gtccagcaac ggccatgccg cgtacatctg ctcgggactt
     8461 tgccgatgtg aagccaccgt tcgccacgag atggctcgtg gccgcgacgc ggctgcggtc
     8521 tctcgactaa tatgatcttt ggtggtggcc atcggctcca agccgacgtg gacggacgag
     8581 caggcgcggg cggtattgcg agtgctcgaa gcagtttctc gtcatcgtga tcgcgaacag
     8641 ctgttcgtcg cgatcgctgg cgttctgcgc tcggtgttca agttcgacgc gttgatcgtc
     8701 aaccttgatg gacccggccc cgatcagatg acgccgtact tcatccaccc acgtgtcgcg
     8761 atcccagcgc tgcagcgctc gcggtccgcg ctcgagaggg tgttcctcac ccaggagccg
     8821 atctacatcc gctcgcgtgc agatgtcgcg gaccgtcctg gctccctcga agcgatggag
     8881 aagttcggcg ctcattccta tgtcggtctg ccgctcgtga tcggcggcaa ggtgctcgcg
     8941 gcgctgctcc tccagtccaa tcgccccaac gcatacgacg atctcgatct gccgttcgcg
     9001 accgagatcg cgtcggccat cgcggtggca ctcgacaatt gtctcgccta cgaacgaatc
     9061 gtgcagtcgc gcgcccggct cgacgccgag aaccagttgc tgcgcgcgga gctacgtgcc
     9121 gcacacctgg ccggccagat ggtcgccgag agcagcgcga tgaccgaggt gaagcggctc
     9181 gtcgagctgg tcgcgccgac cgatgcaacg gtgctgatca cgggcgagac cggtgccggc
     9241 aaggagcgac tcgcgcgatt gatccacgaa cgtagtgcgc gcgccgatcg gccgatgctg
     9301 gcgatcaact gcgcggcgat tccgagctct ttgatcgaga gcgagctgtt cggtcacgag
     9361 gcgggcgcat tcaccggcgc gacgcgtcgc cggcgtggtc gcttcgagct cgcgaaccgc
     9421 ggcaccctct tgctcgacga ggtcggcgag ctggcacccg atgcccaggc caagctgttg
     9481 cgcgtgctcc agacccagga gctcgaacgc gtcggtggca gcgagacgct tcgcgtcgac
     9541 gtgcgcgtga tcgcggcgac gaaccggccg ctcaccgaga tggtggaggc ccgcgagttt
     9601 cgcccggacc tctattaccg gttggcggtg tttccaatcg agctgccgcc actatgggcg
     9661 cgccgcgatg acgttccgat cctcgctcgc cagctcgtcg ccgagagcgc acagcggctc
     9721 ggcatcgccc cgccgcatct cgacgagcag acgattcgcg agctccagcg ctactcgtgg
     9781 ccgggcaacg tgcgcgagct gcagaacgtg atcgagcgcg ctgtgatctt gtcgcgcggc
     9841 ggcctcctcg acgtcgcgcc gctcctcgat ctgtccgggc tcgacgcagc ggacgaggag
     9901 tccgaccaag acgagtccac cgaaggcacc gaagtacgcg ctgctctcga ggcgtcgaag
     9961 tgggtgatcg aaggcgacga cggtgccgcc aaccggctcg gcctgcggcc aagcacgcta
    10021 cgatcgcgca tgcgtcgact cgggatcgaa cggccgcgat gaagctgcac tcggtgctcg
    10081 aggtgctacg cgccatcgct ggccatcgcg aacgacgcga gctgatggcc acgctgctcc
    10141 agacactgcg tgcgtcgatc cgtgccgact actatctggc ggcggtgaag cgcgggcgtg
    10201 gaagcaacga agccgaggca ttcttcgatc cgccgctcac cggggtatcc acgcgcgtga
    10261 gcgactattc gttggtcggt gacctcctgc gtggccgcgc agcgctgttc tatgaacacg
    10321 acatcggaaa ccatccggta cagcgcgcgc tgtgggagcg ctatggcgtc agagtgtcgt
    10381 tgcgcatccc gctcatggtc gagcacgagg ctgtcggtac gatcgcgctg tggtctcgcg
    10441 atcgccacgc attcgacggg ctcgatatgg acgtgatgac ggagctcggc atggtcgtcg
    10501 cggtgacgat cgacagctgt ctcgcgtacg agcacctcgc gcggctgcgc gacgaagccg
    10561 acgatgacaa ctcgtacttg cgcgaagcgc tcgccgacga gctgcagatc agcgaaggca
    10621 tcatcggtgg tggacctgcg ttcgcgacgg tgaagcggca gatcgaggtt gcggcgccga
    10681 ccgagacgac cgtccttttg accggcgaga gcgggactgg caaggatgtc gtcgcgcgtg
    10741 cgatccacgc ggcaagcccg cgccgtgacc ggccgctggt gacggtcaac tgtgccgcga
    10801 tcccgaacaa cctcgccgag accgagctgt tcggccacga ggagggcgcg tttaccggtg
    10861 ccgccaagac gcgcgtcgga cggttcgaac aggcgaacgg cggcacgcta ttcctcgacg
    10921 aggtcggcga gctgccgatg gccgtgcagg cgaagctgtt gcgcgtgatc caggagcgcg
    10981 agctcgaacg cgtgggtggc ggtgacgcga tcaagatcga tgtccggatc atcgcagcaa
    11041 cgaatcgcaa cttgcaggag atgatcaacg agggccgatt ccgcgaggat ctgttcttcc
    11101 gcctcgcggt gtttccgatc catttgccgc cgctgcgctc gcgactcgag gacctacccg
    11161 agctcgtcga acacttcgtc aagctcgcag cagagcggtt ccgcgtgccg gttcgtcgcg
    11221 tcacccccga cgtcgttcgc cggctcgccg accacgattg gcctggcaac gtgcgcgagc
    11281 tccagcacgc gatcgagcgc gcgatgatcg tgagcccgag cgacgcgctc gcgatcgatg
    11341 cggtcgttcc tcgccggatc gctcctccag ttagcagcgg cgccggcagc gatacgctgc
    11401 gtgccgagta tctcgcagcg ctcgaatccg cggagtgggt gatcgagggc gacgacggtg
    11461 ctgctgcgcg gctcggtgtt cacccgaata ctctgcgtca ccgcctcaag cgaatgggca
    11521 ttgcgcggcc gaccgcgcgc tgacgttctg tgagagcgtg gtgggcggga ggttcgcgat
    11581 gagtcctgtc gacgtactcg gtgtcgtcat caagattccg ccggaagcgg tcgttccgct
    11641 gctggtcggc gcaggcgcgc tgttgatcct gatgttcttc ggtgctgcgc tcgcggactg
    11701 gaaaacgaaa catcccgaga cggcgagcaa ggtggggatg gtcatcgcgg tcgcgctcac
    11761 gggttacgcg ctgtatatgg gcgcgacgat cattccggcg aagtacatcg atcacggcgc
    11821 gccgggcgag acgacgatcg ggttcctcga cgggccgatc atgggcttct cggcgctcat
    11881 cggtgcgctg ttctggcggt tcgcgaacca gcgctggctc gcgctcggcg tcggccttgg
    11941 cctcggcctc gcgatgttca tgaagccctt catcctcccg ctgcacggct ggtacgacgg
    12001 tcacgagcat ccgtggcact tcttcgatcc ggagcacttg tcgtttcacg gtccgggagt
    12061 ggccgtgatg atcgcgggtt tgatcgccgg atcgagaccg aagtaactga ttgctttgag
    12121 ctcaaatgag ctcccagttc ttctcgttca caattcttaa cgttgattcg cggcgcgttt
    12181 cacattgcgg cgaacgcgcg attgctagcc tcgattgcgg aggtttacgt atgcgattgc
    12241 attcgtcacg ctgggccgtg ttgctcggca ttgcgatcac gtcctcgtcg ctcgcgctgg
    12301 cacagccgga aacgcgcgat caccggcgac gaccgccacc gccgccgcag gcgccggtgc
    12361 ccgctcccgt cgacaccggt ccgaccgagg cgccgccggc tccgcaagcc gagaccgtca
    12421 cacc
//
LOCUS       DASUFX010000326         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1023751, whole genome shotgun sequence.
ACCESSION   DASUFX010000326 DASUFX010000000
VERSION     DASUFX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023751"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFV99_13120"
     CDS             <1..545
                     /locus_tag="VFV99_13120"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF016400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5060300.1"
                     /translation="QWIAATLAVSIIAALDGGRFYSWFSLVPAKVWHGQLWRLVTWPL
                     VETRPLRLVFACAAIYTFGSQLAIRWGDRRLRRFMLEVVVIAGVATTLVATVAGRSYL
                     RYVGTLAADTSLLIAWSRQFPDAPISYRGVISLHGRDLTLFLLGVIVLLAIYIGPIAM
                     IPEVVACVVAAKYPQSLLRR"
     gene            603..1592
                     /locus_tag="VFV99_13125"
     CDS             603..1592
                     /locus_tag="VFV99_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060301.1"
                     /translation="MLGTLPTFSRGLKIVRIGVFIMLAQLVLGVVMTVKALGINTPDE
                     ARDALKWMEYFFLANVGATAAMFFGVARAIPELKRSGIELGKLIAAAIGFLIATLAML
                     WCYRVISRFVDIMLNPEAGIDSLSSVLSDLKTMKYVAIVKDFAYSFALFSLIGTIRQS
                     AIANDQLALRDYAGSMSRALLVMLVADLFQQLTYGTAGGGIGILGLVGQLLVAGYWIY
                     CHVRLQRFLANSAYFMNEPHNLPVARIVSVPDESPRRAKTSASGPALRPSQPVMRPSQ
                     PSAQRSYPSAPQREVQPTPAAPLAIVAPPPRPEPPRAQSSSEGEAESQPRFLR"
     gene            1760..3097
                     /locus_tag="VFV99_13130"
     CDS             1760..3097
                     /locus_tag="VFV99_13130"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEX5060302.1"
                     /translation="MGSCALTWSVRAVVLLLVLAACSTDEDLSSTEQAVVDPTDFTLP
                     TLTTAQRAAIVHKYDALDPMNQVPRGLLEDAMVYYDVNFAAIPKHSYFVVIDFSLYSG
                     KDRFWLVNTTTGAVEKHKVAHGDGSDPDNNGYADSFGNVSGSNKSSLGFYLTGEIYDG
                     THPHSMRLDGLSPDGSPNGMANTNVRSRAIVVHEASYVSDSNTSQQGRSNGCPALDSD
                     IEVSVVDRIHDGSLMYGAISPLNPPIGRGDGAPCGTIPATGAVIDDGDACFAARGPAT
                     GLHSETSAGMGGSLVWTHATAVANEQNYAEWTLFFDQPGRYSVEVYTDASFAQSRQAH
                     YTVVANGIEHDVTIDQTAANGYQKLGDFDFAQGGGQLVHVGDNTAEASKPQLVFDAVR
                     ITPVSDGSDDGGAADDPGMTDDPGDSSGCAATHSSAGALFGLALLFARRRRRR"
     gene            3178..4473
                     /locus_tag="VFV99_13135"
     CDS             3178..4473
                     /locus_tag="VFV99_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060303.1"
                     /translation="MTTDVELVARCRRRDADAFGALVTRHQQLVFGVALARCQDPALA
                     EDVAQEAFVAAWRDFDRLRDAERVGPWVAGIARNLAANAVRTRARRERIVIEPNAEVP
                     TPEDAALEREDRELLQRALADIPEAHRETLVLFYLQGQAVAEIAGALSISEDLVKQRL
                     SRGRQALRDSVADRVESALTRTRLKPAFRAGVIAALSTASARKAAAGKVIAVMSTQKI
                     IGVVAGVLVVAGGAWWFAAHSKTEQQTAMKATETVANPAATPTVPSQPHAPSKLHVER
                     LPDKNARATLLAAIRDAQTRKSAASSGTSASTSSTPPPALPGVDMDKEYIRGAVREII
                     PLLTECYAQGLERDPKLAGDVVVEFTIEGEPGVGGVIGSSAIDPKATSLGDDSVRECI
                     QETMYAIKIDPPASGGTVTVRYPFAFAPAGEEPPPGPPR"
     gene            4487..>4786
                     /locus_tag="VFV99_13140"
     CDS             4487..>4786
                     /locus_tag="VFV99_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060304.1"
                     /translation="MKGLLATLVVFVAACGGHDPGDIDELLGATCRSDRDCEVRCYLD
                     PQFPGGICSVPCTSDNDCPFEALCMSTSGGMCLYACPDFDCNRLGPGWTCRSRGRE"
BASE COUNT          783 a         1615 c         1568 g          820 t
ORIGIN      
        1 ctcagtggat cgcggcgacg ctcgccgtgt cgatcatcgc ggcgctcgac ggcggccggt
       61 tctacagctg gttctcgctc gtgccggcga aggtctggca cggtcagctc tggcggctcg
      121 tgacgtggcc gctcgtcgag acccgcccgc tgcgtctcgt gttcgcgtgc gccgcgatct
      181 acacgttcgg cagtcagctc gcgatccggt ggggcgaccg gcggctgcgt cggttcatgc
      241 tcgaggtcgt ggtcatcgcg ggcgtcgcca ccacactcgt cgcgacggtc gcgggccgca
      301 gttacctgcg ctacgtcggc acgttggccg cggatacgag tctcttgatc gcgtggtcac
      361 gccagttccc cgacgcgccg atcagctacc gcggcgtcat atcgctgcac ggccgcgacc
      421 tgaccttgtt cctgctcggc gtcatcgtgc tgctcgcgat ctacatcgga ccgatcgcga
      481 tgatccccga ggtcgtggcc tgcgtggtcg cggcgaagta cccgcaatcc ctgctgcgtc
      541 gctgagatag cgggccgcag aaagggcggg catttgccgc ccgagatcgg tagtgtcagc
      601 gcgtgctggg tacccttccg acgttctcgc gtggcctcaa gatcgttcgc atcggcgtgt
      661 tcatcatgct ggcgcagctc gtgctcggcg tcgtgatgac ggtcaaggcg ctcggtatca
      721 acacgcccga cgaagcgcgc gatgcgctga agtggatgga gtacttcttc ctcgcgaacg
      781 tcggcgcgac cgccgcgatg ttcttcggcg tcgcgcgcgc gattcccgag ctcaagcgct
      841 ccggcatcga gctcggcaag ctcatcgccg ccgccatcgg cttcctcatc gcgacgctcg
      901 cgatgctgtg gtgctaccgc gtgatttcgc gattcgtgga catcatgctg aatcccgagg
      961 ccggcatcga ctcgctcagc agcgtcctct cggatctcaa gacgatgaag tacgtcgcga
     1021 tcgtgaagga cttcgcgtac tcgttcgcgc tgttctcgct gatcggcacc atccgccagt
     1081 ccgcgatcgc gaacgaccag ctcgcgctcc gcgactacgc cggcagcatg agccgcgcgc
     1141 tgctcgtgat gctcgttgcc gacctgttcc agcagctgac gtacgggact gccggcggtg
     1201 gtatcggcat cctcggactt gtcggccagc tgctggtcgc cggctattgg atctactgcc
     1261 acgtgcggct gcagcgcttc ctcgcgaact cggcgtactt catgaacgag ccgcacaacc
     1321 tgccggtcgc gaggatcgtg agcgtgcccg acgagtcacc gcgccgagcg aagacatcgg
     1381 cgagcgggcc ggccctgcgc ccatcgcagc cggtcatgcg cccatcgcag ccatcggcac
     1441 aacgatcgta tccctcggcg ccgcagcgtg aggtgcagcc gacgccggcc gcgccgctcg
     1501 cgatcgtcgc accgcctccg cggcccgaac ctccccgcgc gcaaagctcg agcgaaggcg
     1561 aggccgaaag ccagccgcgt ttccttcgct gatcgctctg tcgggtgtgc acacgatcgt
     1621 ttgcaggccg agcgatgcgc agcgggttgc acagtctgcc tgtgtgatga cacctccgca
     1681 gttccgacga ggaattttgt aatcgtcgag tcgccgccga gatgttgacc gcggcggtcc
     1741 acaggcggac tctcgaagca tggggagctg cgcgctgacg tggtcggttc gtgccgttgt
     1801 cctgctgctc gtgctcgccg cgtgttcgac cgacgaggat ttgtcctcga cggaacaggc
     1861 agtcgtcgat ccgacggact tcacgcttcc gaccctcacg acggcgcagc gcgcggcgat
     1921 cgtgcacaag tacgacgcgc tcgatccgat gaaccaggtg ccgcgcggtc tgctcgaaga
     1981 cgcgatggtc tactacgacg tcaacttcgc cgcgattccg aagcacagct acttcgtcgt
     2041 catcgatttc tcgctctact ccggcaagga ccgcttctgg ctcgtcaaca cgacgaccgg
     2101 cgcagtcgag aagcacaagg tcgcgcacgg cgacggcagc gatccggaca acaatggcta
     2161 cgcggactcg ttcggcaacg tgtcgggatc gaacaagagc tcgctcggct tctatctcac
     2221 cggcgagatc tacgacggca cgcatccgca ctcgatgcgc ctcgatggcc tgtcgcccga
     2281 cggcagcccg aacggcatgg cgaacacgaa cgtgcgctcg cgcgcgatcg tcgtgcacga
     2341 agcgtcgtac gtgagcgaca gcaacacgag ccagcaaggc cgcagcaacg gctgccccgc
     2401 gctcgattca gacatcgaag tcagcgtcgt cgatcgcatc cacgacggct cgctgatgta
     2461 cggcgcgatc tcgccgctca acccgcccat cggtcgtggc gatggcgcgc cctgcggcac
     2521 gattcccgcg accggcgccg tgatcgacga cggcgacgcg tgctttgccg cacgtggccc
     2581 ggcgacgggc ctgcacagcg agacgagcgc cggtatggga ggcagcctgg tctggacgca
     2641 cgcgaccgct gtcgcgaacg agcagaacta cgccgagtgg acgctgttct tcgatcaacc
     2701 agggcgctac agcgtcgagg tctacaccga cgcgtcattc gcgcagtcgc ggcaggcgca
     2761 ctacaccgtc gtcgcgaacg gcatcgagca cgacgtcacg atcgatcaga cagcggcgaa
     2821 cggctatcag aagctcggcg acttcgactt cgcgcaaggc ggcggccagc tcgtgcacgt
     2881 cggcgataac acggccgagg cgagcaagcc gcagcttgtc ttcgacgccg ttcgcatcac
     2941 gccggtgagc gatggcagcg atgatggcgg cgccgccgac gatccgggca tgaccgacga
     3001 tccgggcgac agcagtggct gcgcagccac gcacagcagc gcgggcgcgc tgtttggcct
     3061 cgcgctgcta tttgcccgcc gccgtcgccg tcggtagcga gcggcaagcg gaagcggtgg
     3121 caaccgcgta ggatttgggt tgtcaccttt tcgcgtgccg tgaccactac tggcgtaatg
     3181 actaccgacg tcgagctcgt cgcgcgctgc cgtcgccgcg atgcggatgc cttcggtgcc
     3241 ctcgtcacgc ggcatcagca gctggtgttc ggtgtcgcgc tcgcgcgctg ccaggatccg
     3301 gcgctcgccg aggatgtcgc gcaagaagcg tttgtcgccg cgtggcgcga cttcgatcgt
     3361 ctccgcgatg ccgagcgcgt cggtccctgg gtcgcgggca tcgcacgcaa cctcgccgcc
     3421 aacgccgtgc gcacacgcgc gcgccgcgag cgcattgtca tcgagccgaa cgcggaggtg
     3481 ccgacgcccg aagacgccgc gctcgaacgc gaggatcgcg agcttctcca gcgcgcgctc
     3541 gcggacattc ctgaagcgca tcgcgagacg ctcgtgctgt tctatctgca aggccaagcg
     3601 gtcgccgaga tcgccggcgc actgtcgatc agtgaagatc tcgtgaagca gcgactgtcg
     3661 cgtggtcgtc aggcgcttcg cgatagcgtc gcggaccgcg tcgagtcggc gctcacccgc
     3721 acacggctca aacccgcctt ccgcgccggc gtgatcgcgg cgctctcgac ggcaagcgcc
     3781 cgcaaggcag ctgccgggaa agtgattgca gtgatgtcta cgcagaagat catcggcgtc
     3841 gtggccggcg tgctcgttgt cgctggtggc gcgtggtggt tcgcggcgca ctcgaagacc
     3901 gagcagcaga ccgcgatgaa agcgacggag acggtcgcga atccagctgc tacgccgacg
     3961 gtgccatcac aaccgcacgc gccgtccaag ctccacgtcg aacggctgcc cgacaagaac
     4021 gcacgcgcga cattgctcgc agcgattcgc gatgcacaga cgcggaagag tgccgcgagc
     4081 tccggtacga gcgcatcgac gagctcgaca ccgccgccgg cactccccgg cgtcgacatg
     4141 gacaaggaat acatccgcgg cgcggtcaga gagatcatcc cgctgctcac cgaatgctac
     4201 gcgcaggggc tcgagcgcga tccgaagctc gctggcgatg tcgtcgtcga gttcacgatc
     4261 gaaggcgagc ccggcgtcgg tggtgtgatc ggctcgagtg cgatcgatcc gaaagcgacc
     4321 agcctcggcg atgacagtgt gcgcgagtgc atccaggaga cgatgtacgc gatcaagatc
     4381 gatccgccgg caagcggtgg cactgtgacc gtgcgctatc cgtttgcgtt cgcacccgcc
     4441 ggcgaagagc caccaccagg cccgccgcgg tagtacgctc gcctgcatga aaggcttgtt
     4501 agcgaccctg gtcgtgttcg tcgccgcatg cggtggtcac gatccaggag acatcgacga
     4561 actgctcggc gcgacgtgcc gatccgatcg cgactgcgaa gtgcggtgct acctcgatcc
     4621 ccaatttccc ggcggcattt gtagcgtgcc gtgtacctcc gacaacgatt gccccttcga
     4681 ggcgctgtgc atgtcgacga gtggcgggat gtgcctgtac gcgtgcccgg acttcgactg
     4741 caaccgcttg ggacccggct ggacgtgtcg cagtcgtggc cgcgag
//
LOCUS       DASUFX010000327        18945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1162084, whole genome shotgun sequence.
ACCESSION   DASUFX010000327 DASUFX010000000
VERSION     DASUFX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18945
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162084"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1665)
                     /locus_tag="VFV99_13145"
     CDS             complement(49..1665)
                     /locus_tag="VFV99_13145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060305.1"
                     /translation="MADTRNPSGRRPPLLPLPPPPEDLPRLPEVNVDDSAEVRIDHRA
                     LAPNRDSLSGAREVPRRQENTRVGRPTANDRPQGIQRVGPRVIPDTVSPSRKEGSRPP
                     RGMHTPTAPPRSAAIRVTTAQHSSLIGGRYQIVKRIGQGGMGKVYEVNHIHLSRQFAL
                     KIISNTVAETDEARELFYREARFASAMSHPNITSVVDFGEDEKVGMFMVMEFVDGEPL
                     HRILFREKRLSVRKACEIVLQVAEALHYIHKQNVVHCDIKTENILIAEEDLEGKRTKM
                     VAKLLDFGLARPLTGPRASTSLSGTPHYVAPERIRGEPASPASDVYGVGILLYELLTG
                     QVPWDGPVQKILAGHLDEVPKAPSKLIDGGLDPAVENLVLHALAKRPSERHKDMAAFI
                     YELKTVMDMLGIGRRRAAQGPSRRVVIEQKSEKGHKRDELARVAFDACRLPLALLTPS
                     GQIAAANPAFAKFVMGVSVEVEGLPVKSTPLASAWSTCEQDIARTLAGSPIRRIIEID
                     VSATETRRLLLWLDAVSKDHVVLGVQPLEL"
     gene            1701..3878
                     /locus_tag="VFV99_13150"
     CDS             1701..3878
                     /locus_tag="VFV99_13150"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5060306.1"
                     /translation="MQELWSSASYEPHLLSLLFAFAPASMLVVIAYGIAMRGEPVLRA
                     WLLMHFVTLLPLFMTITLAPSIVSPHVATALFRISASCIPLTSVAGGAFQFSLIGKHE
                     ATRWYSLGFLVIACVWLVIGTTTDALCSGVRWLDGAGMWFGVTGDWAWLALLCVIAST
                     LPSYVMLCKAAIFSKPSIERRQLRLVLIANVLTYAGIPVDGSLAFGYGVFPLSWLLGG
                     IGSVLVARALVVEDLLRARAADTTAPNLVLHFAAAILLGWCSLELLGDQVPWWASAMV
                     LAVCFGSVRVLIASLALINRGARGREGTLDRLLGQLVARARPLESEAQIAQLAVDIVE
                     LGLGTRAEVLLAASEDYGWSRPSGEKLDDADAPDPLLGAWLGEHRRALFSDELELHVP
                     HDLREQLSRQFHRHQARALLPISSDGELLALLMVPATFRRVRGRELSFLERAGERFAE
                     ALVHARMAQRAAQRALLSRQVELAATVQAQLLPTKGPHVHGDITVVGTWLPASRCAGD
                     FWGVYPLGERRVLVAIGDVAGHGIASSMVTAAAAAAVDVAVRRYGKDLELTNLITALD
                     AAVRRVGGGQLSMTAFAAVLDPDAREIRFISCGHTTPYLVRPGDELELQALVGRGNPL
                     GGGGSVGAKVLQKPLKAGDLVVWYTDGVIEAQDAGGEAFGDRRLQRMLRRLDRAQFTA
                     PAVHNVLYASVAAHRSGVPREDDETLVVAQFATPRAASTEAAQ"
     gene            3875..4663
                     /locus_tag="VFV99_13155"
     CDS             3875..4663
                     /locus_tag="VFV99_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5060307.1"
                     /translation="MRLRVLSYNIHKCIGGVDRRYQPQRVADVCAALDADVLMLQEVD
                     AGVTRSNGDRQVDVLGDLIGMRYRTWFPNVDVRGGGHYGNAILSRYPLIESTNIDLSI
                     RFKKKRSVLHGVIRVRHDDVDRTVHLYNMHLGLARYERRIQLGMFLDSHPFANLHHDT
                     PVVVGGDLNDVYGRLGELLRPAGFRGVDRRPLTFPAWGPVRPLDAIFVRGHVDFLKLS
                     RCESELARRASDHRPLLAEVRLQPHQAHLSSHHHHEHKPPTPRP"
     gene            4708..5292
                     /locus_tag="VFV99_13160"
     CDS             4708..5292
                     /locus_tag="VFV99_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5060308.1"
                     /translation="MRLAVALGVLAACSFDHGIPVNAQDDAPVAMIDSKSIDASGSGS
                     ATPDARPDAAPDARACPSNFQPIANGPAGSVYRIHGYSQVNDQSLPFQTARTGCVNEG
                     GYLAIPNDANEIMALLAATPRESMMPWMWVGITDQAQEGTWLTVLGTTPYLVWGAGEP
                     DGGTANNCLLLAQTNQMYDSTCGSSWAYACECEQ"
     gene            complement(5293..6984)
                     /gene="ettA"
                     /locus_tag="VFV99_13165"
     CDS             complement(5293..6984)
                     /gene="ettA"
                     /locus_tag="VFV99_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX5060309.1"
                     /translation="MTAQYTFVMKDLRKLVPPKREILKGIWLSFYPGAKIGVLGNNGA
                     GKSTLLRIMAGVDKDFLGEAWPAPGLKVGYLAQEPQLDPAKTVKENVDEGVAETRKLL
                     TRFEEISMALGEDMPEAKMNKLLEEQGRLQDQIDAVNAWELDHQVDMAMDALRCPPGD
                     ADVTKISGGEKRRVALCRLLLSNPDMLILDEPTNHLDAESVAWLEKTLETFKGTVVAV
                     THDRYFLDNVAKWILELDRGEGHPFEGNYSAWLELKAKRLKDEERQADARQKMLAREL
                     EWVRSSPKARQAKSKARLARYDEMVNEAQKEARAEGLEIVIPPGPRLGGDVVSFDGVY
                     KSYGDRLLIENLTFKLPRGGIVGVIGPNGAGKTTLFRMIMGVEKPDKGTIKIGETVKL
                     GYVDQSRDALDDNKTLYDEIAEGAETLTLGEPPNQRTMNARAYVSSFNFKGQDQQKKV
                     GILSGGERNRVHLAKMIKRAGNVLLLDEPSNDLDVDTLRALEDGLEKFPGCAVVISHD
                     RWFLDRIATHILAFEGDSHVEWFEGNFQDYEADRHRRLGDAADQPHRIKYKPIRT"
     gene            7016..7846
                     /locus_tag="VFV99_13170"
     CDS             7016..7846
                     /locus_tag="VFV99_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060310.1"
                     /translation="MAYRGDDAGDAVEAPTAEGLLKVLLGPNRVDLSVAQRSLHIADR
                     IATLVEHKKQKQDRRGSVKILGNLVVARGFPREDVGLWIEHLGDDKKKKSDDKRKAEP
                     AEDDKRKPPSKADDSRADEPSMQRIFGVEPVSLLERDGLGALGRLDVLAQRLRAHLAD
                     LAGPIRRAIEIGSGHALDKVLLADHGDHYMFYARRLFRDRARFAMAVHDDGRIVVPDG
                     KTLREVRVTSRFGITVRGDYIRFADPHGTDLAKVSLPWLGPEDRDEVARRIGQFVDRE
                     "
     gene            complement(7847..10609)
                     /locus_tag="VFV99_13175"
     CDS             complement(7847..10609)
                     /locus_tag="VFV99_13175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060311.1"
                     /translation="MIAAGAWIYHYELIRELGRGAMGTVWAARDTKLGRRVAIKVLRD
                     AAQEVADRFLVEARATAQCNHDNIVIIHEVGAINGTPYMVFEFLEGDTLREVMGGFST
                     GQRLAPSRVVELALPIARALVRAHELGIVHRDLKPENVFVTSAGQVKVLDFGIAKAFA
                     EHPRRLTQYGTQRGPEPRSLQMTTEGELVGTLPYMSPEQMTGVDVDHRADLYALGVIL
                     FEMLTGYHPVQPLTMDAMVANAHAPTPLASVHTRAPDVPDALAQLVERCLRKQRFERI
                     ANAAEVVRTLEALLPGRHGRQLAEGESPYPGLIAFQEADADRFFGRGRDVTRMVARVR
                     ELPLTGVVGPSGVGKSSFIRAGVGPALKSSGERWEIVTLRPGRQPVAALASVVQRLAS
                     RANVQTQFDEQHHRLRNEPGYFGTCMRGYARQTGQSVLLFVDQFEELYTLMPDAGERR
                     AFTAALTGAADDSAAPVRVVVSMRSDFLDRIAEDPRFMEDLSRGLVFLSPPDRVGLRE
                     ALIAPVEMVGYRFETMEMIEDMLDALVGTPGALPLLQFAAAKLWDTRDRQRRLLTVAS
                     YTAIGGISGALATHADEVVARMTAPAQRLTQKIFQRLITPERTRAIVELADLHELSPD
                     VGHVLDQLVAARLLVVQTRGDTAGGTVEIVHESLIGRWPTLRRWLDEDQEDTAFLAQL
                     SAAAKQWEAKRHAVGVLWRGEAMEEARRWFALRPRELSPRDRAFLDAVLALARRGQRT
                     RRTVLVGAFILLGAIAAAAIVALVWIRGAEQNATDALSAMQLKERERLAAEADKAAAE
                     QRKAAAEADTKAAEASKAAAEADKAAAEAARAKAQEQVVAKDTIIEGNREELQRQNVK
                     LKAALDAASAAKENAEREQARAQKAAADAQKAAAELEKANAILQKKNDELEREKAKLS
                     TKLKGL"
     gene            complement(10672..11646)
                     /locus_tag="VFV99_13180"
     CDS             complement(10672..11646)
                     /locus_tag="VFV99_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060312.1"
                     /translation="MKLVAIIVFALLASPAFAGNEPWSTGVSDQQKQAAQKLLEQGNA
                     LFLEKNFSAALEKYKEAVAAWDHPAIRFNMVRCLIQLERPVEASDNLQLALEYGAAPL
                     DEAVYNEALNYQKLLGKQLGDLEVSCTQAGVKVSFDGQPLVTCPGTTKRRATAGQHMI
                     VGTKDGFLTKTVEAVVLGGKQQRVEVALIPLETAAKITHRWPGWVPWSVFGGGLAIAG
                     VGGLLQLQAASRMDSYDKQVTRNCSDIHCDPNDPTMLDTSDKQSAERLSVIAISVMSV
                     GAAAAITGGVMLYMNRGRTVYEKSVEKAARIDVVPTKGGGLVTVGGSF"
     gene            complement(11643..13745)
                     /locus_tag="VFV99_13185"
     CDS             complement(11643..13745)
                     /locus_tag="VFV99_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060313.1"
                     /translation="MRGLILVLVAAGCVRTTAVTCSDGHVCAPDKVCDNTHETCVFPD
                     QLTSCVDRADGEACNINGAPGGICKDKVCIPATCGDELVSGDEVCDGATDIPDVGTCV
                     DFAYDYGALGCNALCTEGFSTCRRLGWQARSLNGVGGIAGIWEDGADLWVANQQGIYH
                     RHAGVWEPLIQPTGSVVWYRSIWAGGGHVFAAGYDGSIAHFNGTSWDSAETGTLHVNG
                     IWGRSPTDVYAVGGKQGGQIFHFDGNGWTEMTIPTAGLLNGVFGIGNDVFAVGDAGTI
                     LRLVGGSWTPMNVSAGGYNLRGVWGSGPTDVFAVGTPSADGPPGIALHYDGNQWTTMA
                     LPAGTTQLNAITGRSISEVYAVGVGAVSNILFYDGTGWIPMVTSVASANLSLQAVHAG
                     ATTVYAAKRPSLFLEYAGVGRLDTPSLPNEVRGLWGTNKGYAIAVGDGVFFQEWAQGK
                     WTSVPYPSCWPGTTFVFGAWGSAGSDVWIVGPYGNMMHRTATGTTCVNAPNVKWILDI
                     WGFAANDIYAVGADGSTGEANLLHYNGTTWEDQSSRVMPLGKSLSGVWGSGPSDVYAT
                     AEAKLLHYNGTAWSDAGLVTTHKLTKVWGASASDVFVVGEGGAIYHYDGTAWTQMIVP
                     LATTTPTDDAWSDVNGTGANDVWVVGNRTLLHYDGTSWSPVSRLSAGAAEAVWSMPGA
                     VVVGGELGIDARLIGRLQ"
     gene            complement(13766..16192)
                     /locus_tag="VFV99_13190"
     CDS             complement(13766..16192)
                     /locus_tag="VFV99_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5060314.1"
                     /translation="MRRISWLVAISACGFSGGNDSKPPTVVSVSPGDGADGVWLHDPI
                     TVTFSEAIDPASVTATSVVVTADDGTPLAATATLTGAVLTVQVADDAAAVGAVRLTLS
                     NAITDMDANPLAETSVGWTLPAWSRAATTGATPSLAIGDGGRVVVAWSAGANSARRVV
                     VNQIGPAGFVALGGELGAGDSALPSITFDAQDRPIVVWSQFGATASVQAARWDGDAWV
                     PLASPGDGAYAVAARSPGGDPMIAYVAAGSFQNGSAVVVRRLVDDAWQPLGTDARDIA
                     VIGTVSGLPQLAMATPDTPVVAFADTGATGTSGRGPAVRVLRWTGTWIETTIPIGVPV
                     QNGSETTGAFNRVAVAAHGDTIDVAYDTYSGFSWGVHVARVDAQGYHLLGGELDVDSP
                     ADAQAPAIALDADGTPIVAWRELVEGNWRGFVARWDGTAWRAVGGGAWNDDAAHAIVR
                     PVMALAGGRAPVIAWGAQSVDSMSASATIAIARWNGPAVPRFGIEARTSITGCAFDGT
                     QTTLSATGCFTIANGRATPHAGLIPFDIVSELWSDGARKRRWIALPDGEMMTTQPTGA
                     WTPPAGAMLIKEFAIETTPGDPRTRRVMETRFLIVGASSGVPSLQGYSFRWRIDGSDA
                     DLLPDAATTVDWPLDNGTLHTHSYPSRAQCLRCHNVSNGPLLGVRTGQLARRFDYDGV
                     MGDQLDTLAHIGAIAPQLEVQPFASPHDATLPLETRMRGYVAANCSHCHNPGGERPTR
                     DFRWEVPLAQTKLCGTDAEVVAGDPASSVIFQRISTRVNGMPPLATLQTDPLAIDVIG
                     RWIQSEVNCQ"
     gene            16338..18107
                     /locus_tag="VFV99_13195"
     CDS             16338..18107
                     /locus_tag="VFV99_13195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5060315.1"
                     /translation="MPRLVVHEPGGVTTAVEVDGDVRIGRETGLEVSLLDTKVSRHHA
                     TIAKDGANWVLTDAGSRHGTFVNGQRVTRHVLAEGDQLQIGATQMRFEHATTPSSVAL
                     HLTTAVPPQIDERLRVFYQLADATAAIEEGDAALRPALAAIVAVLGCDRGVIALGNLP
                     TNLRRAAEVEARGLVISRAVIESVIVRGEAVLLRGADAALQMTLDRQGVRSAIAAPLR
                     IRDRTIGLVYVDDRGRDDRFEQADLELLVAVARLAGVIVDVAGRYERAVAIVENVERE
                     RVAPELIGSSAELGRVRLEVARFAATELPIFLTGESGVGKELVARALHATSPRKAAQF
                     IAVNCAAMPDTLLESELFGHVRGAFTGADRPRRGKLVLADGGTLFLDEVADLSLAAQA
                     KLLRVLEDGEVVPVGSEQAIKTDLRVVSASHKDLQQAVTEGRFRQDLYYRLVGAEVAI
                     PPLRERARDVVELAETFLARVRAKRQAPSRFSPDAITALTTYGWPGNVRELRHAVERA
                     CAIAAGDVIEAADLSLRAPTGKLGESRDTLANRYAALDDHERQLVEEAMAKAGNNVAE
                     AARLLGITRIMMKRRLDRFSGNE"
     gene            complement(18108..18569)
                     /locus_tag="VFV99_13200"
     CDS             complement(18108..18569)
                     /locus_tag="VFV99_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5060316.1"
                     /translation="MSQHPRAGLLAEIEFHENSARMGDAAGSQLGHVAAWAEDNFDGL
                     IVVDGHADAHGPAKNNVALSMRRARLVRDQLIAIGVDPTQIVISAFGAETRRHARVRV
                     WGTHNSLESVIAGRRNAKEVLVNDDRVLELQQRQARPSPMRTRRSHTCCAQ"
BASE COUNT         2974 a         6491 c         6183 g         3297 t
ORIGIN      
        1 agcacggcag cgacgacgag caatggcctc atgggccggg accctagcct agagctcgag
       61 cggctgcacg ccgaggacta cgtgatcctt cgagacagcg tctagccata ggagcaacct
      121 gcgcgtctcg gtcgccgaca cgtcgatctc gataattcgg cggatcgggc tgccggcgag
      181 cgtgcgtgca atgtcttgtt cgcaggtgga ccacgcggag gccagcggcg tcgatttgac
      241 cggcaggccc tcgacctcga cggagacgcc catgacgaac ttcgcaaatg ctggatttgc
      301 tgcagcgatc tgccccgacg gcgtgagcag cgcgagcggg agtcggcagg cgtcgaacgc
      361 gactcgtgcg agctcgtcgc gcttgtggcc cttctcagat ttttgctcga tgacgacacg
      421 gcgcgacggc ccctgcgcag cacgcctgcg gccgatgccg agcatgtcca tcacggtctt
      481 cagctcgtag atgaacgcgg ccatgtcctt gtgccgctcg ctcggccgtt tcgcgagcgc
      541 gtgcagcacg aggttctcga cggcaggatc gagccctcca tcgatcagct tcgacggtgc
      601 cttcggtacc tcgtcgagat ggccggcgag aatcttttgc accgggccgt cccacggcac
      661 ctgcccggtg agcagctcgt agagcaagat gccgacgccg tagacgtcgc tcgccgggga
      721 tgcgggctcg ccgcggatgc gctcgggcgc aacataatgc ggcgtacctg acagcgacgt
      781 cgacgcgcgc ggaccggtga gcgggcgcgc gaggccgaag tcgagcagct tggcgaccat
      841 cttggttcgc ttgccctcga gatcctcttc agcgatcagg atgttctcgg tcttgatgtc
      901 gcagtggacg acgttctgct tgtggatgta gtggagcgcc tcggcaacct gcaggacgat
      961 ttcgcaggct ttacggacgg acaaccgctt ctcgcgaaac aagatgcgat ggagtggttc
     1021 gccgtcgacg aactccatca ccatgaacat gccgaccttc tcgtcttcac cgaagtcgac
     1081 gaccgacgtg atgttcggat gcgacatcgc cgatgcgaag cgagcctcgc gatagaacag
     1141 ctcgcgcgcc tcgtcggtct cggcgaccgt gttcgagatg atcttcagcg cgaactgacg
     1201 cgacaggtgg atgtgattga cctcgtacac cttgcccatg ccgccctgac cgatgcgctt
     1261 gacgatctgg tagcgaccgc cgatcagcga gctgtgctgc gcggtcgtca cgcggatggc
     1321 ggcgctgcga gggggcgcgg tcggcgtatg catgccgcgc ggcgggcgcg agccctcctt
     1381 gcggctgggt gacacggtat cggggatcac gcgggggccg acgcgctgga tcccttgcgg
     1441 gcggtcgttt gcggtcggcc tgccgactcg ggtgttttcc tggcgacgcg gcacctcgcg
     1501 ggcgcccgag agcgagtcac ggtttggcgc gagcgcacga tgatcgatcc gcacctcggc
     1561 ggaatcatcg acgttgacct cgggcagccg cggtaggtcc tccggcggcg gaggcagtgg
     1621 cagtaacggc gggcggcggc ccgagggatt gcgcgtatcc gccacgcctg aatcgtacac
     1681 gaggtacagt ggttaggtcg atgcaggagc tgtggtcgag cgcgagctac gagccgcacc
     1741 tcttgtcatt gcttttcgcg tttgcgccgg cctcgatgct cgtcgtgatc gcctacggca
     1801 tcgccatgcg cggcgaacct gtcctgcgcg cctggctgct gatgcacttc gtcaccctgc
     1861 tgccgctgtt catgacgatc acattggcgc catcgatcgt ctccccccat gtcgcgaccg
     1921 ccttgtttcg catctctgcc agctgcatcc ccctgacctc cgtcgccggc ggcgcctttc
     1981 agttcagtct gatcggcaag cacgaggcca cacggtggta cagcttggga tttctcgtaa
     2041 tcgcatgcgt ctggctggtc attggtacga ccacggacgc gctctgcagc ggggtgcgct
     2101 ggctggacgg tgctggcatg tggttcggcg tcactggcga ctgggcctgg ctggccctgc
     2161 tgtgcgtcat cgcgagcacg ctgccgagct acgtgatgct gtgcaaggcg gcgatcttca
     2221 gcaagccgtc gatcgagcgc cgtcagctgc gcctcgtgct catcgcaaac gtgctcacgt
     2281 atgccggcat ccccgtcgac ggcagtctcg cgttcggcta cggcgtgttt ccgctgtcgt
     2341 ggctgctcgg tgggatcggc agcgtgctcg tcgcacgagc gcttgtcgtc gaggaccttc
     2401 tgcgggcacg cgcggccgac acgacggcgc cgaacctcgt gctgcatttc gcggctgcga
     2461 tcctgctcgg ctggtgctcg ctcgagctgc ttggcgacca ggtgccgtgg tgggcgagcg
     2521 cgatggtgct cgcggtctgc ttcgggagcg tgcgcgtgct gatcgcgtcg ctcgctctca
     2581 tcaaccgcgg tgcgcgcggg cgcgaaggca cgctcgatcg cctgctcggc cagctcgtcg
     2641 cgcgtgctcg cccgctcgaa tccgaagcgc agatcgccca gctcgcggtc gacatcgtgg
     2701 agcttggcct cggtacgcgc gccgaagtgt tgctcgccgc ctctgaagac tatggctggt
     2761 cacggccgtc gggcgaaaag ctcgacgacg ccgatgcgcc cgatccgcta ctcggcgcat
     2821 ggcttggtga acatcggcgc gcgctgttct ccgacgagct cgagctgcat gttcctcacg
     2881 atcttcgtga acagctcagc aggcagtttc atcgtcacca ggcgcgcgcg ctcctgccga
     2941 tctcgagcga cggcgagctc ctcgcgttgc tgatggttcc cgcgacgttt cgccgcgtgc
     3001 gcggacgcga gctgtcgttc ctcgaacgcg ccggcgagcg gtttgctgaa gcgctcgtcc
     3061 acgcgcgcat ggctcagcgc gcagcgcaac gcgccctgct ctcgcgtcag gtcgagctgg
     3121 ccgccaccgt gcaagcgcag ctgctaccga cgaagggacc gcacgtgcac ggcgacatca
     3181 ccgtcgtcgg aacgtggctg cccgcgtcgc ggtgcgccgg cgatttctgg ggcgtgtatc
     3241 cgctcggcga gcgccgcgtg ctcgtcgcga tcggtgacgt cgccggccac ggcatcgcat
     3301 cgtcgatggt gaccgcagcc gcggctgctg ccgtcgatgt cgcggtgcgc cggtacggca
     3361 aggatctcga gctgacgaac ttgatcaccg cgctcgatgc cgcggtccgc cgcgtcggcg
     3421 gtggccagct ctcgatgacg gcattcgcgg cggtcctcga tccggacgcg cgtgagattc
     3481 gcttcatctc gtgcggccat acgactccct atctcgtgcg gcctggcgac gagctcgagc
     3541 tccaggcact cgtcggccgc ggcaatcccc tggggggcgg cggcagcgtc ggtgcgaagg
     3601 ttctgcaaaa accactgaag gccggcgatc tcgtcgtctg gtataccgat ggtgtgatcg
     3661 aggcccagga tgctggtggc gaggcgttcg gtgatcgcag gttgcagcgg atgctgcgcc
     3721 gtctcgatcg cgcgcagttc accgcaccgg ccgttcataa cgtgctgtac gccagcgtcg
     3781 ccgcgcatcg ctccggcgtt ccgcgcgagg atgacgagac gctcgtagtc gcgcagtttg
     3841 caacgccgcg cgccgcgtcg acggaggccg cgcaatgaga ctgcgcgtcc tcagctacaa
     3901 catccacaaa tgcatcggcg gcgtcgaccg ccgctatcaa ccgcaacgcg tcgccgacgt
     3961 gtgcgccgcg ctcgatgcgg acgtgctgat gctgcaggaa gtcgacgccg gcgtgacgcg
     4021 gtcgaacggc gatcgccagg tcgacgtgct cggcgatctg atcggcatgc ggtatcggac
     4081 gtggttcccg aacgttgacg tgcggggcgg cgggcactac ggcaacgcga tcctcagccg
     4141 ctatccattg atcgagagca cgaacatcga tctgtcgatc cgcttcaaga agaagcgcag
     4201 cgtgctgcac ggcgtgatcc gcgtgcgcca cgacgacgtc gaccgcaccg tgcatctcta
     4261 caacatgcac ctcgggctcg cgcgttacga gcgtcgcatc cagctcggca tgttcctcga
     4321 cagccatccg ttcgcgaacc tccaccacga cacgccagtc gtcgtcggtg gtgatctcaa
     4381 cgacgtctac ggccgactcg gcgagctatt gcgtcccgcc ggatttcgcg gtgtcgatcg
     4441 gcgcccgctc acgttcccag catggggtcc ggtgcggccg ctcgacgcga tcttcgtgcg
     4501 cggccacgtc gacttcttga agctttcccg ctgcgagagc gagctcgcgc gccgggcaag
     4561 cgaccaccgt ccgctcctgg cggaggttcg cctccaaccc catcaggcgc acctatcaag
     4621 tcatcaccat cacgaacaca agccgccaac cccgcggccc tgatgattcg ctgatcgaca
     4681 ggcgctcggc ggtgatacgc tgagggtgtg cgactggccg tagccctcgg cgtgctcgca
     4741 gcttgctcgt tcgaccatgg cattccggtc aacgcgcagg acgacgcgcc cgtggcgatg
     4801 atcgacagca agtcgatcga cgcgagcggt agtggcagcg ccacgcccga cgcgcgtccc
     4861 gacgccgcgc cggatgcgag ggcatgcccg agcaacttcc agccgatcgc gaacgggccg
     4921 gccggctcgg tctaccggat ccacggctac tcgcaggtca acgatcagag cctgccgttc
     4981 cagaccgcac gaaccgggtg cgtgaacgaa ggtggctatc tcgcgatccc gaacgacgcg
     5041 aacgagatca tggcactgct cgccgcgacg ccgcgcgaga gcatgatgcc gtggatgtgg
     5101 gtcgggatta ccgatcaagc gcaggaaggc acgtggctga cagtgctggg cacgacgccg
     5161 tacctcgtct ggggcgctgg cgagcccgac ggcggcacgg caaacaactg cctgctgctc
     5221 gcgcagacca atcagatgta cgactcgaca tgcggcagct cgtgggcgta cgcgtgcgag
     5281 tgcgagcagt gactaggtcc ggatcggctt gtacttgatc cggtgcggct ggtcggcggc
     5341 gtcaccgagc cggcgatggc ggtcggcttc gtaatcctgg aagttgccct cgaaccactc
     5401 gacgtggctg tcgccctcga acgcgaggat gtgcgtcgcg atgcgatcga ggaaccagcg
     5461 atcgtggctg atgacgaccg cgcagcccgg gaacttctcg aggccgtcct cgagtgcgcg
     5521 cagcgtgtcg acgtcgaggt cgttcgacgg ctcgtcgagc agcagcacgt tgccggcgcg
     5581 cttgatcatc ttcgcgaggt gcacgcggtt gcgctcacca ccggagagaa tgccgacctt
     5641 cttctgctgg tcctggccct tgaagttgaa gctcgacacg tacgcgcgag cgttcatcgt
     5701 gcgctggttc ggcggctcgc cgagcgtgag cgtctcggcg ccttcggcga tctcgtcgta
     5761 gagcgtcttg ttatcgtcga gcgcgtcgcg cgactggtcg acgtagccga gcttgacggt
     5821 ctcgccgatc ttgatcgtgc ccttgtcggg tttctcgacg cccatgatca tgcggaacag
     5881 cgtcgtcttg ccggcgccgt tcgggccgat cacgccgacg atgccgccgc gcggcagctt
     5941 gaacgtcagg ttctcgatca gcaggcgatc gccgtatgac ttgtagacac cgtcgaagct
     6001 gacgacgtcg ccaccgaggc gcggtcccgg cgggatcacg atctcgagcc cctcggcgcg
     6061 cgcttctttt tgcgcctcgt tcaccatctc gtcgtagcga gcgagacgcg ccttgctctt
     6121 ggcctggcgc gccttcggcg agctgcgcac ccactcgagc tcgcgcgcga gcatcttctg
     6181 acgtgcgtcg gcttgccgct cctcgtcctt gaggcgcttc gccttcagct cgagccacgc
     6241 cgaataattg ccttcgaacg ggtggccctc gccgcggtcg agctcgagga tccacttcgc
     6301 gacgttgtcg aggaagtaac gatcgtgcgt gaccgcgacg acggtcccct tgaacgtctc
     6361 gagcgtcttc tcgagccacg cgacggactc ggcgtcgagg tggttcgtgg gctcgtcgag
     6421 aatcagcatg tccgggttcg agagcagcaa gcggcacagc gcgacgcggc gcttctcacc
     6481 accggagatc ttcgtgacgt cggcgtcgcc cggagggcag cgcagcgcat ccatcgccat
     6541 gtcgacctga tggtcgagct cccacgcatt caccgcgtcg atctgatcct ggagacgacc
     6601 ttgctcctcg aggagcttgt tcatctttgc ctcgggcatg tcctcgccga gcgccatcga
     6661 aatctcttcg aagcgcgtaa gcagcttgcg cgtctcggca acgccctcgt cgacgttctc
     6721 cttgacggtc ttcgccggat cgagctgcgg ttcctgcgcg agataaccga ccttgagacc
     6781 cggtgccggc cacgcttctc cgagaaagtc cttgtcgacg cccgccatga tgcggagcaa
     6841 cgtgctcttg ccggcaccgt tgttaccgag gacgccgatt ttcgcgcctg gataaaacga
     6901 caaccaaatg cctttgagga tctcgcgctt cggcggcacg agcttgcgga gatccttcat
     6961 cacgaacgtg tactgggcgg tcatggcggg cggggttata ccgtaccctt ggcgcgtggc
     7021 ctaccgaggc gacgacgcag gcgatgccgt agaagcaccg acggcagagg gcctgctgaa
     7081 ggtgctcctc ggtccgaacc gcgtcgatct atccgtcgcg caacgtagcc ttcacatcgc
     7141 cgaccgcatc gcgacgctgg tcgagcacaa gaagcagaag caagaccgcc gcggctcggt
     7201 gaagattcta ggaaacctcg tggtggcgcg cgggtttcca cgcgaggatg tcggcctgtg
     7261 gatcgagcac ctcggcgacg acaagaagaa gaagagcgac gacaagcgca aggccgaacc
     7321 ggccgaggac gacaagcgca agccgccgag caaggctgac gactcccgcg cggacgagcc
     7381 gagcatgcag cggatctttg gcgtcgagcc ggtgagcctg ctcgaacgcg acgggctcgg
     7441 cgcgctcggc cgcctcgacg tgctcgcgca gcgcctccgc gcccacctcg ccgacctcgc
     7501 cggaccgatc cgccgcgcca tcgaaatcgg atccggccac gcgctcgaca aggtgctgct
     7561 cgccgatcac ggcgaccatt acatgttcta cgcgcgccgg ctgtttcgcg accgagcgcg
     7621 gttcgcgatg gcggtgcacg acgacggccg cattgtcgtc cccgacggca aaacgctgcg
     7681 cgaggtgcgc gtgacgtcgc ggttcggtat caccgtgcgt ggcgactaca tccgcttcgc
     7741 cgacccgcac ggcaccgacc tcgcgaaggt gtcgctgccg tggctcggcc ctgaagatcg
     7801 cgacgaagtc gcgcgtcgca tcggccaatt cgtcgatcgg gagtagctac agccccttca
     7861 gcttcgtcga cagcttcgcc ttctcgcgtt cgagctcgtc gttcttcttc tgcaggatcg
     7921 cgtttgcctt ctcgagctcc gccgctgcct tctgggcgtc ggcggcggcc ttctgtgccc
     7981 tcgcttgctc gcgctcggcg ttttccttcg cggcgcttgc ggcgtcgagc gccgccttca
     8041 gcttcacgtt ctggcgctgc agctcttcgc gatttccctc gatgatcgtg tccttcgcga
     8101 cgacttgctc ttgcgccttc gcgcgagccg cttcggcggc ggctttgtca gcctccgcgg
     8161 ccgccttgct cgcttcggct gctttcgtgt ccgcctccgc agcagccttg cgttgctctg
     8221 cagcggcttt gtccgcttct gccgcgaggc gttcgcgctc cttgagctgc atcgccgaca
     8281 gcgcatccgt cgcgttctgc tcggcgccgc ggatccacac cagcgcgacg atcgccgcgg
     8341 ctgcgatcgc gccgaggaga atgaacgcac cgacgagcac ggtgcgccgc gtgcgctgac
     8401 cgcgccgggc gagtgcaagg accgcgtcga gaaatgcgcg atcgcgcggc gacagctcgc
     8461 gcggccggag agcgaaccag cgccgcgctt cttccatcgc ttcgccgcgc cacagcacgc
     8521 cgactgcgtg gcgcttcgct tcccattgct tcgcagcggc cgacagctgc gcgaggaacg
     8581 cagtgtcctc ctgatcttcg tcgagccagc ggcggagcgt tggccagcgg ccgatcagcg
     8641 actcgtgcac gatctcgacg gtgccacccg cggtatcgcc gcgcgtctgc acgacgagca
     8701 agcgcgccgc gacgagctga tcgaggacgt ggccaacgtc gggcgacagc tcgtgcaagt
     8761 cggcgagctc gacgatcgcg cgcgtgcgct cgggcgtgat caggcgctgg aagatcttct
     8821 gcgtgagccg ctgcgccggt gccgtcatcc gcgcgacgac ctcgtcggcg tgggtcgcga
     8881 gtgcgccgct gatgccgccg atcgcggtgt agctcgcgac cgtcagcaag cggcgctggc
     8941 gatcgcgcgt gtcccacagc ttcgcggctg cgaactgcaa gagtggcaac gcgcccggcg
     9001 tgccgacgag tgcgtcgagc atgtcttcga tcatctccat ggtctcgaac cggtaaccga
     9061 ccatctcgac cggcgcgatc aacgcttcgc gtagaccgac gcgatcgggc ggcgacaaga
     9121 agacgagccc gcgcgagaga tcctccatga accgcgggtc ttcggcgatg cgatcgagga
     9181 agtccgagcg catcgacacg acgacgcgca cgggtgcagc gctgtcgtcg gcggcgccgg
     9241 tcagcgcagc ggtgaacgca cgccgttcgc ccgcatccgg catcaacgtg tacagctctt
     9301 cgaactggtc gacaaacagc agcacgctct ggccggtctg tcgcgcgtaa ccgcgcatgc
     9361 acgtgccgaa gtagcccggc tcgttgcgca gccgatggtg ctgctcgtcg aactgcgtct
     9421 gcacgttcgc gcgactcgcg agtcgctgca cgacgctcgc cagcgccgcg acaggttgcc
     9481 ggcccggacg aagcgtcacg atctcccaac gctcgcccga gctcttgagc gcgggaccga
     9541 cgccggcgcg aatgaacgat gacttgccga cacccgacgg accgacgaca ccggtgagcg
     9601 gcagctcgcg cacgcgcgcg accatgcgcg tgacatcgcg gccacgcccg aagaagcgat
     9661 ccgcatccgc ttcctgaaat gcgatgaggc cgggatatgg gctctcgccc tcggcgagct
     9721 ggcgaccatg gcggccgggc agcagtgctt cgagcgtgcg cacgacctcg gcagcgttcg
     9781 cgatgcgttc gaaccgttgt ttgcgcaggc agcgctcgac gagctgggcg agggcatcgg
     9841 ggacatcggg cgcgcgcgtg tgcaccgacg cgagcggcgt cggtgcatgc gcgttcgcga
     9901 ccatcgcgtc catcgtcagc ggctgcaccg ggtgatagcc ggtcaacatc tcgaacaaga
     9961 tcacgccgag cgcgtagagg tcggctcgat gatcgacgtc gacgccggtc atctgctcgg
    10021 gcgacatgta cggcagcgtg ccgacgagct caccttcggt cgtcatttgc agcgagcgcg
    10081 gctcggggcc acgctgcgtg ccgtactgcg tgagccgccg cgggtgctcg gcgaacgcct
    10141 ttgcgatgcc gaagtcgagg accttgacct ggcccgcgct cgtgacgaac acgttctcgg
    10201 gcttgagatc gcgatggacg ataccgagct cgtgagcgcg cacgagcgca cgtgcgatcg
    10261 gcagcgcgag ctccacgacg cgcgacggcg cgagccgctg tcccgtcgag aacccgccca
    10321 tcacttcgcg tagcgtgtcg ccctcgagga actcgaacac catgtacggc gtgccgttaa
    10381 tcgcgccgac ctcatggatg atgacgatgt tgtcgtgatt gcactgcgcg gtcgcgcgcg
    10441 cttcgacgag gaagcgatcc gcgacctctt gcgctgcgtc gcgcagcacc ttgatcgcga
    10501 cgcgccggcc gagcttcgtg tcgcgcgctg cccataccgt acccatcgcg ccgcggccga
    10561 gctcgcgaat cagctcgtag tgatagatcc atgcacccgc agcgatcaca ccgggcgctt
    10621 gctgcggcgg tgattggacg accggtactt cccagtcggt cgtgcgcgcg gtcagaacga
    10681 gcctccgacg gtcacgagcc cgccgccttt cgtcggcacg acgtcgatgc gcgctgcctt
    10741 ctcgacgctc ttctcgtaca cggtgcgtcc gcgattcatg tagagcatca ccccgcccgt
    10801 gatcgcagcc gcggcgccga ccgacatcac gctgatcgcg atcacgctga gccgctcggc
    10861 gctctgcttg tcgctcgtgt cgagcatcgt cggatcgttc ggatcgcagt ggatgtccga
    10921 gcagttgcgc gtgacttgct tgtcgtagct gtccatccgc gaggccgcct gcagctggag
    10981 cagaccgccg acgccggcga tcgcgagacc gccaccgaac accgaccacg gcacccaacc
    11041 cggccagcga tgcgtgatct tcgcggcggt ctcgagcgga atcagcgcca cttcgacgcg
    11101 ctgctgcttg ccgccgagca cgacggcttc gacggtcttc gtgaggaacc cgtccttcgt
    11161 gccgacgatc atgtgctgac cggcggtcgc gcgtcgcttc gtggtgcccg ggcacgtgac
    11221 gagtggttga ccatcgaagg agaccttcac gccggcctgc gtgcagctca cttccagatc
    11281 gccgagctgc ttgccgagca acttctgata gttcagcgct tcgttgtaga cagcttcgtc
    11341 gaggggcgcc gcgccgtact cgagcgcgag ctggaggttg tccgatgcct cgacgggacg
    11401 ctcgagctgg atcaagcagc gaaccatgtt gaagcggatc gcggggtgat cccacgctgc
    11461 gaccgcttcc ttgtacttct cgagcgccgc actgaagttc ttctcgagga agagcgcgtt
    11521 gccttgctcg agtaacttct gcgctgcttg cttctgctgg tcgctgacgc ctgtgctcca
    11581 cggctcgttg ccggcgaacg cgggcgaggc gaggagcgcg aacacgatga tcgcgacgag
    11641 cttcattgca gcctcccgat caaccgcgcg tcgatgccga gctcaccgcc aacgaccacc
    11701 gcgcctggca tcgaccacac tgcctccgca gctccggcag acaggcgaga caccggcgac
    11761 cacgacgtgc cgtcgtagtg aagcagtgtg cggttgccga cgacccacac gtcgttcgcg
    11821 ccagtaccat tgacgtcact ccatgcgtcg tccgtcggcg tcgtagtcgc gagcggcacg
    11881 atcatctgcg tccacgcggt gccgtcgtaa tgatagatcg caccaccctc accgacgacg
    11941 aacacgtcgc tcgcgctcgc cccccacacc ttggtgagct tgtgcgtcgt gacgagcccc
    12001 gcatcgctcc acgctgtgcc gttgtagtgg agtagcttcg cctcggcggt cgcgtatacg
    12061 tcgctcggcc ccgagcccca cacgccagac agtgatttcc cgagcggcat cacgcggctc
    12121 gattggtcct cccacgtcgt accgttatag tggagcaggt ttgcttcgcc ggtcgagccg
    12181 tcggcaccga cggcatagat gtcgttcgcg gcgaatcccc agatgtcgag gatccatttc
    12241 acgttcggcg cgttcacgca cgtggtcccc gtcgcggtcc tgtgcatcat gtttccgtac
    12301 ggcccgacga tccacacgtc actgccagcc gacccccagg cgccgaacac gaaggtagta
    12361 cccggccagc agctcggata cggcaccgac gtccacttgc cctgcgccca ctcctggaaa
    12421 aacacgccat cgccgaccgc gatggcgtag cccttgttcg tgccccacag gccgcgcacc
    12481 tcgtttggca gcgatggggt atcgagacgc cccacgcccg catactcgag aaacaagctc
    12541 ggccgcttcg ccgcatacac cgtggtcgcg ccagcatgca ccgcctgcag actgaggttc
    12601 gccgacgcga cactcgtcac catcggaatc cagccggtgc cgtcgtagaa caggatgttg
    12661 ctcacggcgc caacgccgac tgcgtagact tcactgatcg agcggccggt gattgcattg
    12721 agctgcgtcg tcccagcggg aagcgccatc gtggtccatt gattaccgtc gtagtgcagc
    12781 gcgatccccg gtggaccgtc tgcagacggc gtaccgaccg cgaacacgtc cgtcggtccg
    12841 ctgccccaga ctccgcgcag gttgtagccg cccgcgctca cgttcatcgg cgtccatgat
    12901 ccgccgacga gccgcaggat tgttcctgca tcgccgaccg cgaacacgtc attgccgatg
    12961 ccgaacacac cgttgagcaa ccccgccgtg ggaatcgtca tctccgtcca cccgttgccg
    13021 tcgaagtgga agatctggcc gccttgcttg ccgccgaccg cgtacacgtc ggtcgggctt
    13081 cgcccccaga tgccgtttac gtgcagggtg ccggtctcgg cgctgtccca cgaggtgcca
    13141 ttgaagtgag cgatcgatcc gtcgtacccg gcggcgaaca cgtgaccacc gccggcccag
    13201 atgctgcggt accagaccac gcttcccgtc ggttggatca gcggttccca cacaccggcg
    13261 tgccggtgat agatgccttg ctggtttgcg acccagagat cagcaccgtc ttcccaaatt
    13321 cccgcgatgc cacccacacc gttgagagag cgtgcctgcc agccgagccg gcggcacgtg
    13381 ctgaatcctt cggtgcacag cgcgttgcat ccgagcgcgc cgtagtcgta cgcgaagtcg
    13441 acgcacgtgc cgacatcggg gatgtcggtc gcgccatcgc agacctcgtc gcccgagacg
    13501 agctcgtcac cgcacgtcgc aggaatgcag accttgtcct tgcagatgcc gcccggcgcg
    13561 ccgttgatgt tgcacgcctc gccatcggct ctgtcgacgc acgacgtgag ctggtcggga
    13621 aacacgcacg tctcgtgcgt gttgtcgcag accttgtccg gcgcgcaaac atggccatcg
    13681 ctgcacgtca ccgccgtggt ccggacgcac cccgccgcca ccagtaccag tatgagtccc
    13741 cgcatggtct ttagggtaac gccgatcact ggcaattgac ctcgctttgg atccatcgcc
    13801 cgatcacgtc gatcgcgagt ggatcggtct ggagcgtcgc aagtggcggc attccgttca
    13861 cgcgcgtgct gatgcgctga aagatcacgg atgacgccgg atcgccggcg acgacttccg
    13921 catcggtgcc gcacagcttc gtctgcgcga gcgggacctc ccagcggaag tcgcgcgtcg
    13981 ggcgctcgcc acccggattg tggcagtgcg agcagttcgc agcgacgtag ccgcgcatcc
    14041 gcgtctcgag cggcagcgtc gcgtcgtgcg gcgacgcgaa cggctgcacc tccaactgcg
    14101 gcgcgatcgc gccaatgtgc gcgagcgtgt cgagctgatc ccccatgacg ccatcgtagt
    14161 cgaagcgtcg cgcgagctga ccggtacgta cgccgagcag tggcccgttc gacacgttat
    14221 ggcaacgcag acattgcgca cggctcggat agctgtgcgt gtggagcgtg ccgttatcga
    14281 gcggccagtc gaccgtcgtc gccgcatcgg gcaacaggtc tgcgtcactg ccgtcgatgc
    14341 gccagcggaa gctgtagccc tgcaagctgg ggacacccga cgatgcgcca acgatcaaga
    14401 agcgcgtctc catcacgcga cgcgtacgcg gatcaccggg cgtggtctcg atcgcgaatt
    14461 ctttgatcag catcgcaccc gcgggcggcg tccacgcacc ggtgggctgc gtcgtcatca
    14521 tttcgccgtc gggcagtgcg atccagcggc gcttgcgcgc gccgtcggac cacagctcgc
    14581 tcacgatgtc gaaggggatc aaaccggcgt gcggcgtggc gcgaccgttc gcgatcgtga
    14641 agcagccggt cgcgctgagc gtggtctgcg tgccgtcgaa cgcgcagccc gtgatcgacg
    14701 tgcgggcctc gatgccgaac cgcggcaccg cggggccgtt ccagcgtgcg atcgcgatcg
    14761 tcgcgctcgc gctcatcgag tcgaccgatt gcgctcccca cgcgatcacc ggcgcgcggc
    14821 cacccgcgag cgccatcaca ggacgcacga tcgcatgcgc ggcatcgtcg ttccacgcac
    14881 cgccgccgac cgcgcgccat gccgtgccgt cccagcgcgc gacgaagccg cgccagttac
    14941 cctcgacgag ctcgcgccac gcgacgatcg gcgtgccgtc ggcgtcgagc gcgatcgccg
    15001 gcgcttgtgc atccgcgggc gagtcgacat cgagctcgcc gccgagcaga tggtagccct
    15061 gcgcgtcgac gcgcgcgacg tgcacgcccc acgagaaccc gctgtacgtg tcgtacgcga
    15121 catcgatcgt gtcgccgtgc gccgcgaccg caacgcgatt gaacgcgccg gtcgtttcgc
    15181 tgccgttctg caccggaacg ccgatcggga tcgtggtctc gatccacgtg ccggtccagc
    15241 gcagtacacg gacggcaggc ccgcggccgc tggtccccgt cgctccggta tcggcaaacg
    15301 ccacgaccgg cgtgtccggt gtcgccatcg cgagctgcgg caatccactg acggtgccga
    15361 tgaccgcaat gtcgcgcgcg tcggtgccga gcggttgcca cgcgtcgtcg acgagccggc
    15421 gcacgacgac cgcgctgccg ttctgaaatg acccagcagc gacgtacgcg atcatcggat
    15481 cgccacccgg tgaccgcgcc gcgacggcat acgccccatc gcccggtgac gcgagcggga
    15541 cccatgcgtc gccgtcccag cgcgcggcct gcacggacgc ggtggcgccg aactgcgacc
    15601 acacgacgat cggacggtcc tgcgcgtcga acgtgatcga cggcaacgcc gagtcacccg
    15661 cgccgagctc gccgccgagc gcgacgaatc ccgccgggcc gatctggttc acgaccacgc
    15721 gccgcgccga gttggcaccc gcgctccacg ccacgacgac gcgcccgcca tcaccaatcg
    15781 cgagcgacgg tgtcgcgccg gtcgtcgcgg cacggctcca cgccggcagc gtccagccca
    15841 cgctcgtctc cgcgagcgga ttggcatcca tgtccgtgat cgcgttggac agcgtcagcc
    15901 gcacggcgcc gaccgccgcg gcatcgtccg cgacttgcac ggtcagcacg gcgccggtca
    15961 gcgtcgccgt cgcggccagc ggcgtaccgt catccgccgt cacgacgacc gacgtcgcgg
    16021 tcacgctggc cggatcgatc gcctcggaga acgtgaccgt gatcgggtcg tgcagccaga
    16081 cgccgtcggc gccgtcaccc ggcgataccg acacgaccgt gggtggtttg gagtcgttgc
    16141 caccggaaaa gccacacgcg gaaattgcga caagccagga aatgcgacgc atggtcgtcc
    16201 cgccttcgca agcgccatgc cgcgtgcaac tgtgcgagct tgctcggctc ctcgccgggg
    16261 cgcgatcgat ccgatcgatc caaagcgatc attcgatcaa tcaatcggcg tggccgaaag
    16321 tgatacaagc gagggtgatg ccacgccttg tcgtgcacga gccgggcggc gtcacgacag
    16381 cggtcgaggt cgatggcgac gtacggatcg gtcgcgaaac cggtctcgaa gtcagcttgc
    16441 tcgacaccaa ggtgtcgcga catcacgcga cgatcgcgaa ggacggcgcg aattgggtgc
    16501 tgaccgacgc cggctcgcgg cacggcacgt tcgtcaacgg ccagcgcgtc acgcgccacg
    16561 tgctcgccga gggcgaccag ttgcagatcg gcgcgaccca gatgcgcttc gagcacgcga
    16621 ccacgccatc gtccgtggcg ctgcacctca cgaccgcggt gccgccgcag atcgacgaac
    16681 gcctgcgcgt gttctatcaa ctcgccgatg cgaccgcggc gatcgaggaa ggcgacgccg
    16741 cgctgcgccc tgccctcgcg gcgatcgtcg cagtgctcgg ctgcgatcgc ggcgtgatcg
    16801 cgctcggcaa cctaccgacc aatctgcggc gcgccgccga ggtcgaggcg cgcgggctcg
    16861 tgatctcgcg ggcggtgatc gaatcggtga tcgtgcgcgg cgaagcggtg ctgcttcgcg
    16921 gtgccgatgc cgcgcttcag atgacgctcg atcggcaggg cgtgcgctcg gcgatcgccg
    16981 ccccgctacg cattcgcgat cgcacgatcg gtcttgtcta cgtcgacgat cggggccgcg
    17041 acgatcgctt cgaacaagcc gacctcgagc tgctcgtcgc ggtcgcccgg cttgccggcg
    17101 tgatcgtcga cgtcgccggt cgctacgaac gcgccgtcgc gatcgtcgag aatgtcgaac
    17161 gcgagcgggt tgcccccgag ctgatcggtt cgagcgccga gctcggacgc gtgcgcctcg
    17221 aggtcgcgcg gttcgccgcg accgagctgc cgatctttct caccggcgag agcggtgtcg
    17281 gcaaagagct cgtcgcgcgc gcgctccacg cgacctcgcc acgcaaagcg gcgcaattca
    17341 tcgcggtcaa ctgcgcagcg atgcccgaca cgctgctcga gagcgagctg ttcggtcacg
    17401 tgcgcggcgc gtttaccggc gccgaccgcc cccggcgcgg caagctcgtg ctcgccgacg
    17461 gcggcacgct gttcctcgac gaggttgcgg acctcagcct cgccgcgcag gcaaagctgc
    17521 tccgcgtgct cgaagacggc gaagtcgtgc cggtcggcag cgagcaggcg atcaagaccg
    17581 acctgcgcgt tgtctcggcg tcgcacaagg atctgcagca agcggtcacc gagggtcgct
    17641 tccgacaaga tctctactac cgactcgtcg gcgccgaggt cgcgatcccg ccgctgcgcg
    17701 aacgcgctcg cgatgtcgtc gagcttgccg aaacgttcct cgcgcgcgtc cgtgcgaagc
    17761 gtcaggcgcc gagtcggttc tcgcccgatg cgatcacggc gctcaccacg tacggctggc
    17821 ccggcaacgt ccgcgagctc cgccatgcgg tcgagcgcgc gtgtgcaatc gcagccggag
    17881 atgtgatcga agccgcggac ctgtcgctgc gcgcgccgac cggcaagctc ggcgagagcc
    17941 gcgacaccct cgcgaaccgc tacgccgcgc tcgacgatca cgagcgtcag ctcgtcgaag
    18001 aagcgatggc gaaggcgggc aataacgtcg ccgaggccgc gcgcctgctc ggcatcacgc
    18061 gcatcatgat gaagcgccgg ctggatcgat tctctggcaa cgaatagcta ctgggcgcaa
    18121 cacgtgtgcg agcgccgtgt gcgcatcggc gacggtcgtg cttgccgctg ctgcagctcg
    18181 agcactcgat cgtcgttgac gagcacctcc ttcgcgttgc gccgtcctgc gattaccgat
    18241 tcgaggctgt tgtgtgtgcc ccacacgcgg actcgcgcgt gcctgcgagt ctctgcgccg
    18301 aatgccgaga tcacgatctg cgtcgggtcg acaccgatcg cgatcagctg atcacgcacg
    18361 aggcgcgcgc gccgcatgga cagcgctacg ttgttcttcg cagggccgtg ggcgtcggcg
    18421 tggccatcga cgacgatcaa tccgtcgaag ttgtcttccg cccacgcggc gacgtgcccg
    18481 agctgagagc ccgcggcatc gcccatccgc gccgagttct catggaattc gatttccgcg
    18541 agcaggccgg cccgaggatg ctgcgacatg ttcccgggct cggcacctac gctcagcgga
    18601 acgagtacta ctactacaag caaagctaat ctcatcgcgt ccctctccga aggaggtcga
    18661 gatgaagggc cgcaacccat gtgccgccta ctacccgttt gcgtaggacg attcggggaa
    18721 tatttccgtc ggtgtactgc cctcggcagg ccctttacat cgacgtaagc ggacccacaa
    18781 tgaccccatg ctgaagtcct ggcggctcgg aaccatcctc gggtttccgg tcgagctcaa
    18841 cctgagcttc ttgctcctgc ttgcactcgt gttcttcgcg ttcggcggcg cgatcggagt
    18901 cgcgctcgtg ttgctcgtgt tcggctcggt gctgttgcac gagct
//
LOCUS       DASUFX010000328         9377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1300680, whole genome shotgun sequence.
ACCESSION   DASUFX010000328 DASUFX010000000
VERSION     DASUFX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300680"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..981)
                     /locus_tag="VFV99_13205"
     CDS             complement(97..981)
                     /locus_tag="VFV99_13205"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX5060317.1"
                     /translation="MVTVLVVGASGEVGRCVVQSLTHRGARVRVLVHSTPFDGAESVR
                     GDLADPASLASALAGCDGACFITPHHEHEAQLGFNFVDACEAAKLRRLVFVSSFHPCT
                     WLPLQAVLDGIVGVIGPHYRPKFQVERRVRSSPLSPVVLGPSNFYQNDELALPEILAG
                     SYPNPMGKRRLNRVDVRDIGDGAARSLVDDDVASGAYPIVGPDNWTGAAVAQVWSEAL
                     GRPVTYGGDDVERWRSTVGARMQPARRADDFARTYRIIQRFGAPATTRTIAKTTALLG
                     RPPRDFRTYCAELAARTR"
     gene            complement(983..1921)
                     /locus_tag="VFV99_13210"
     CDS             complement(983..1921)
                     /locus_tag="VFV99_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5060318.1"
                     /translation="MKKILKRVAIGVAMFLVLGTAAVAIAVVARENRTFDAPYPDIHA
                     SRDPDVIERGRYLVTGPAHCVECHGMVGDTNEERHLVGGLTFNLPIGTIYARNITPDQ
                     GTGIGKYTDPEVARVLRYGVHPSGRAMMPFMPFADMTDEDLTAVISYLRSRPPIDHYV
                     PPSDFNFLGRAAKAFLLEPKGPSQPIRAHVERGPTAEYGKYIANTIANCNGCHTRRNL
                     RNGDAEGVTFAGGMSLESHSAPGTKFVTPNLTPDPKTGHIYVWSEDVFVARFKNAVPT
                     ASPMPWSTFRKMSEDDLRALYRYFRSLPPARMGQDL"
     gene            1981..2508
                     /locus_tag="VFV99_13215"
     CDS             1981..2508
                     /locus_tag="VFV99_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060319.1"
                     /translation="MGVVAVSLADEAALCRRYAPAVRTFWRRRLRGTEVDELTQETLL
                     RFVQALRRGAITDPERMGGFVLGICRNLARERVRSAERRSELWAEFAGALASLADEPE
                     VASYQLAQLEDCLSQMTQRSRDVIRHAFIDGHSSSQIGQRMGMSEGNVRVVRHRTLEA
                     LRTCMSQKVFWEAPS"
     gene            2505..3038
                     /locus_tag="VFV99_13220"
     CDS             2505..3038
                     /locus_tag="VFV99_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060320.1"
                     /translation="MTRLYELDAYLTGELNEGEADALEEAMFGAPDDPDLLFLDRIAR
                     HGAQLAEHGTFDIGVPRAHIDTLVAAGHKVQMFDPGPPGKGHSLILDDDAEFVVTMLA
                     IGRNDIERVDVEINLIDYNATKTIKDVTVDRDGIVYGLCERPLAKLAFGAGRTVANVR
                     RTDGARDVIATWDLTPP"
     gene            complement(3311..6427)
                     /locus_tag="VFV99_13225"
     CDS             complement(3311..6427)
                     /locus_tag="VFV99_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5060321.1"
                     /translation="MFISNYAIKKPIITTVVMVALVVFGLIALVRLHTDEFPEVTAPV
                     VNVAIVYPGASPGVVERELINPLEESFRSISGVDTINSTAVDGYANILVQFVFEKDIQ
                     QATQDIRDKISESRADLPIEMEEPILTRFASSDFPIISLTLTSTTVAPVRLTELADQR
                     IRRELTSLQGVASVDIVGGVERELTVEVQPEALRASGVSISQVVQTLQTENLAVPVGK
                     LSGKLEDRTIRLRGRLSGPSEFEQLVVGRSGDRVVRLGDVAKVRDGQEEPLTAAFFNK
                     TPAVGIDIKKALGYSTTDVSQRVLKRIAELQQDPQLKGTKLEIVRDSGARVDASVKDV
                     QTSLFEGALLTILVVFIFLKSWRSTVITGLALPVSVIASFVAVLAFGFTLNVMSLLGL
                     SLAIGILVDDAIVVRENIVRHMEMGKDHVRAAAEGTSEIGLAVAATTFSIVVVFVPIA
                     FMGGVAQQWFAPFALTIACSVLVSLFVSFSLDPMLSAVWHDPEAAGQHSWLTKRLMFF
                     DRAFQALTRRYKKIIAWALRHRFVMVVLALVTFFGALAIPATGAVGSSFFPIEDRSEF
                     QISLECAPSSNLEYTQKKVLAAVDLALKRPEVMYAYSTIGGRSGADTAQIYVRLKPKK
                     ERKLHQDVVAQALRNDIRQLSGVTAYITTGFAGGFKQIQIQLIGPDTDILNGIAERVA
                     KEVRQVPGAVDVGLSSKGQRAEVEIIPDRDLAGQLGISAAVLAQAVRPAFAGLESGHW
                     IDPDGETRDVVVRLAPDWRQRPEDLEALPISLPQFGPQVARTIRLGEIAKVKRGFGPA
                     QIEHLDGSRVVTVGANAEGVPLSAVVGAIEPRLAKMPMPDGYQRKQGGETQDQQEVFG
                     RILIALGTAIMLMYFILVIQFGSFLEPIPIMASLPMSLIGVMLAMLVTGSTLNLMSMI
                     GVIMLMGIVAKNAILLIDFAKQAEKNGLSREEAIVEAGAVRLRPIVMTSVAIVAGMIP
                     VAIGSGEGGDFRAPLGRAVIGGVITSTVLTLLAIPTFYDIITHWRDAVIRRVRRKKPS
                     PPPGTESH"
     gene            complement(6427..7662)
                     /locus_tag="VFV99_13230"
     CDS             complement(6427..7662)
                     /locus_tag="VFV99_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5060322.1"
                     /translation="MRLFTIIVIVIALAACKKGAQEQPKSTNEVPTIKLAPEDIVVLA
                     RGELQTGPRISGTLQAASRAVVRAETQGSVVAIGPELGQPVKKGDLLARIEAKALGDV
                     ASSAKSGLESAQAQYDLALREVQRTEALVKGGALAARELDRAKSELSAATAAVTQARA
                     QVASSQSQLGDATVKAPLAGVVARRAISVGDVVSPGSELYEVIDPSTMRLDASVASED
                     LSVIALGKAVDFEVRGYPGQLFTGKIQRIAPAADPVTRQIQVLVDIPNPGGKLIAGLY
                     AEGRIAVERREALTAPVGAIDSAGDQPTVLRVKNSVVERVIVALGVRDERAEVVEVTS
                     GLAPGDVLVLARAQKNVGPGAKVELPQQGAKTEDKPAAPDEKSAAPDEKPQAPAAKPT
                     TPDEKPADKKAPAAGSAGK"
     gene            complement(7670..9043)
                     /locus_tag="VFV99_13235"
     CDS             complement(7670..9043)
                     /locus_tag="VFV99_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5060323.1"
                     /translation="MMLRLSFVAGLCSLTLAGSAAAQPPSATPESPTALSFEEAVGAA
                     ERTAESVAIANADVDRADAQVTSARAGYLPTVNSSLAYQRTLASEFDDINFGPMTGGS
                     DVKLPFGQRNNWRVNLNVTQPIFDGFRTKNAVDVAKGGVRHAELGVNSMRAQVALQVA
                     QAYFDAVLAERQVVIGEETLRQAEQTYQETELGFKQGTRPEFDLVRAEVVRDNQRTLV
                     VQFKVQRDVTLVSLRRLVGLPLDRQLQLTSTLDSNDLEQLLATARKAAGITSTNRIAV
                     AQAKESVGIRESALKLAKSQYWPSLSAGTDLGFVDYDNTPFHPDWRTNWTLGVTLSLP
                     VFDGLRREAQVRTTQAELAAAKQQLQLSSKVSNVETAQARASVEASQTQLENSIRTVK
                     QAQRAYEIAELRFSQGASTHLEITDARVQLEQALLVQARAARDLRVARFREELLPALP
                     LGLQLVF"
     gene            complement(9167..>9377)
                     /locus_tag="VFV99_13240"
     CDS             complement(9167..>9377)
                     /locus_tag="VFV99_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060324.1"
                     /translation="SQQHPPSVPPYTTPIPGSIPPTTYPQGSMPPQSQLVPRPTSSGR
                     GRRIFVILAAAIIVSTIGILIVLLT"
BASE COUNT         1604 a         3081 c         3078 g         1614 t
ORIGIN      
        1 gaaggcgagc gcagcgatcg ccgacgaagc gggtaccgac gccgcggcgc ttccagtacg
       61 aagaagagga gcccgcgacg tggacgaaga agtgagctag cgggttcgcg ctgcgagctc
      121 ggcgcagtac gtgcggaagt cgcgtggcgg tcggccgagc agcgcggtcg tcttcgcgat
      181 cgtgcgcgtg gtcgcgggcg caccgaagcg ctggatgatc cggtatgtcc gtgcgaagtc
      241 atccgcgcgc cgcgccggct gcatgcgcgc accgacggtc gagcgccaac gttcgacatc
      301 atcgccgcca tacgtcaccg gccggccgag cgcctcgctc cacacctgcg cgaccgcagc
      361 gccggtccag ttgtcggggc cgacgatcgg gtaggcaccg gatgcgacat cgtcatcgac
      421 gagcgagcgc gcagcgccat caccgatgtc gcgcacgtcg actcggttga ggcggcgctt
      481 gcccatcggg ttcggatagc taccggcgag aatctcgggc agcgcgagct cgtcgttctg
      541 atagaagttt gacggcccga gcacgacggg cgacagcggc gacgagcgca cgcggcgttc
      601 gacctgaaac ttcgggcgat agtgcggacc gatcacgccg acgattccgt cgagcacggc
      661 ctgaagcggc aaccacgtgc acgggtggaa tgacgacacg aacacgagac ggcgcagctt
      721 cgccgcttcg cacgcgtcga cgaagttgaa gccgagctgt gcctcgtgct cgtggtgcgg
      781 cgtgatgaag caggcgccgt cacaaccggc aagcgcgctc gcgagcgatg cagggtcggc
      841 gagatcaccg cgcacagact cggcaccgtc gaacggcgtc gagtgaacga gaacgcgcac
      901 gcgtgctccg cggtgcgtga gcgactggac gacgcagcgg ccgacttcac cggacgcgcc
      961 gacaacgagg acggttacca cgttagagat cttgccccat tcgcgccggc ggaagcgaac
     1021 ggaagtaccg atagagtgcg cgcagatcgt cttcgctcat cttgcggaac gtcgaccatg
     1081 gcatcgggct cgccgttggc actgcgttct tgaagcgcgc gacgaacacg tcctcactcc
     1141 atacgtagat gtggcccgtc ttcggatctg gcgtgaggtt cggcgtcacg aacttcgtgc
     1201 cgggcgcgct gtgcgactcg aggctcatgc cgccggcgaa cgtgacgccc tcggcgtcgc
     1261 cgttgcgcag gttgcgccgc gtgtggcagc cgttgcagtt cgcgatcgtg ttcgcgatgt
     1321 acttgccata ctccgcggtc ggcccacgtt cgacgtgcgc gcgaatcggt tgactcggac
     1381 ctttcggctc gagcaagaac gccttcgccg cgcggccgag gaagttaaaa tcgctcggcg
     1441 gcacgtagtg atcgatcggc ggtcggctac gcagatacga gatcaccgcg gtcagatcct
     1501 cgtcggtcat gtccgcgaac ggcatgaacg gcatcatcgc gcggccactt ggatgcacgc
     1561 cgtagcgcag cacgcgggcg acctcggggt cggtgtactt gccgatgccc gtcccttggt
     1621 cgggtgtgat gttgcgcgcg tagatcgtgc caatcggcag attgaacgtg agaccgccga
     1681 cgagatgacg ctcctcgttc gtgtcgccca ccatgccgtg gcattcgaca caatgcgcgg
     1741 gaccggtcac gagatagcgg ccacgctcga tgacgtcggg atcacggctc gcatggatgt
     1801 cgggatatgg agcgtcgaac gtgcggttct cgcgcgcgac caccgcgatg gcgaccgcgg
     1861 ccgtacctag caccaggaac atcgccacac cgatggcgac gcgcttcagg attttcttca
     1921 tgccccgcca agtgcggtga gccgatgctt cgttacaaag tttctgttac ggtgacgcaa
     1981 atgggcgtag ttgctgtatc gctagccgac gaggccgccc tgtgccgtcg ctatgcgccc
     2041 gccgttcgca cgttttggcg ccgtcggcta aggggaaccg aggtcgatga gctgacgcag
     2101 gaaacgctgc ttcgcttcgt ccaggcactg cggcgtggcg cgatcaccga ccccgagcgg
     2161 atgggcgggt tcgtgctcgg catctgccgc aatctcgcgc gcgaacgcgt gcgcagcgcg
     2221 gagcgccgtt ccgagctgtg ggctgaattc gccggcgcgc tcgcctcgct cgcagacgag
     2281 ccggaggtcg ccagctatca gctcgcgcaa ctcgaagact gcttgagcca gatgacgcag
     2341 cgctcgcgcg acgtgattcg tcacgcgttt attgatggcc acagttcctc ccagatcggg
     2401 caacgtatgg gcatgagcga aggcaatgtg cgcgtcgttc gccatcgcac gctcgaagcg
     2461 ctgcgcacgt gcatgagcca gaaagtgttc tgggaggcac cgtcgtgacg cgcttgtacg
     2521 agctcgacgc ctacttgacg ggcgagctga acgagggcga ggccgacgcg ctcgaagaag
     2581 cgatgttcgg cgcgcccgac gatccggacc tgctgttctt ggatcgcatc gcacgccacg
     2641 gtgcacagct cgccgagcac ggcacgttcg acatcggcgt gccgcgcgct cacatcgaca
     2701 cgctcgtcgc tgccggccac aaagtgcaga tgttcgatcc cgggccgccc ggtaaaggcc
     2761 actcgttgat tctcgacgac gatgccgagt ttgtcgtcac gatgctcgcg atcggccgca
     2821 acgatatcga gcgcgtcgat gtcgagatca acctcatcga ttacaacgcg acaaagacga
     2881 tcaaggacgt caccgtcgat cgcgacggca tcgtctacgg cctgtgcgag cgtccgctcg
     2941 ccaagctggc attcggtgcc gggcgcacgg tcgcgaacgt ccgccgtacc gacggcgcac
     3001 gcgacgtgat cgcgacctgg gacctgaccc cgccctaggc gcttcgcgga cgcgctgtcc
     3061 gagccgggtc cgggtgccgg acggcaaaaa ctaaccccgg tacgtcgggg gaacggtgtt
     3121 tcaggatctt aaccaacccc ggtacggcgc tacggacggc aaaactaacc ccggtacgtc
     3181 gggggaacgg tgtttcagga tcttaactaa ccccggtacg gcgctactgg tgcgcgcgca
     3241 cggtcgcgaa cgtccgccgt accgacggcg cacgcgacgt gatcgcgacc tgggacctga
     3301 cgccaccgcc ttagtgcgat tcggttcccg gcggcggtga cggcttcttc cggcgcaccc
     3361 ggcggatcac cgcatcacgc cagtgcgtga tgatgtcgta gaacgtcggg atcgcgagca
     3421 gcgtgagcac ggtggacgtg atcacaccgc cgatcacggc gcggccgagc ggtgcgcgga
     3481 aatcgccgcc ttcacccgag ccgatcgcga ccgggatcat gccggcgacg atcgcgacac
     3541 tcgtcatcac gatcggtcgt aggcggaccg cgcccgcttc gacaatcgct tcctcgcgcg
     3601 acaggccgtt cttctcggcc tgcttcgcaa agtcgatcag aaggatcgcg ttcttggcca
     3661 cgatgcccat cagcatgatc acgccgatca tcgacatgag gttgagcgtc gagccggtga
     3721 cgagcatcgc gagcatcacg ccgatcagcg acatcggcag tgacgccatg atcggaattg
     3781 gctcgaggaa cgagccgaac tggatcacga ggatgaagta catgagcatg atcgccgtgc
     3841 cgagcgcgat caggatgcgg ccgaacacct cttgctggtc ttgcgtctcg ccgccctgct
     3901 tgcgctggta gccgtcgggc atcggcatct tcgcgaggcg tggctcgatg gcgccgacga
     3961 ccgcggagag cgggacgccc tcggcgtttg caccgacggt gacgacgcgc gagccgtcga
     4021 ggtgctcgat ctgcgctggc ccgaagccac gtttgacctt cgcgatctcg ccgaggcgaa
     4081 tcgtgcgcgc gacttgcggt ccgaactgcg gcaagctgat cggcagcgct tcgagatctt
     4141 cgggacgttg ccgccagtcc ggcgcgagcc gcacgacgac gtcacgcgtt tcgccgtctg
     4201 gatcgatcca gtggccggat tcgaggccgg cgaacgcagg ccgcaccgcc tgcgcgagca
     4261 cggccgcgct gatcccgagc tggccagcga ggtcgcggtc cggaatgatc tcgacttcgg
     4321 cgcgctggcc cttgctcgag aggccaacgt cgaccgcgcc cggcacctgg cgcacttctt
     4381 ttgcgacgcg ctccgcgatg ccgttgagga tgtcggtgtc gggaccgatc agctggatct
     4441 ggatctgctt gaagccgccg gcgaatcccg tcgtgatgta cgcggtcaca cccgacagct
     4501 gccggatgtc attgcgcagc gcctgcgcga cgacgtcctg gtgcagcttg cgctccttct
     4561 tcggtttgag ccgcacgtag atctgcgccg tgtccgcgcc cgaccggcca ccgatcgtcg
     4621 agtacgcgta catgacctcg gggcgcttga gcgcgaggtc gacggctgcg agtaccttct
     4681 tctgcgtgta ctcgaggttg ctgctcggcg cgcactcgag cgagatctgg aattcggagc
     4741 ggtcttctat cgggaagaag ctgctaccga cggcgccggt cgccggaatc gccagcgcac
     4801 cgaagaacgt gacgagcgcg agcaccacca tcacgaaccg gtgacgcagc gcccacgcga
     4861 tgatcttctt gtagcgccgc gtcagtgcct ggaacgcgcg gtcgaagaac atcaaccgct
     4921 tcgtcagcca cgagtgctgg cccgcggcct cgggatcgtg ccagaccgcg gacaacatcg
     4981 ggtcgagcga gaacgacacg aacagcgaca cgagcaccga gcacgcgatc gtgagcgcga
     5041 acggcgcgaa ccactgctgc gcgacgccac ccatgaacgc gatcggcacg aacacgacga
     5101 cgatcgagaa cgtggtcgct gcgaccgcga gcccgatctc cgacgtgcct tctgccgcgg
     5161 cgcgcacgtg gtctttgccc atctccatgt ggcgcacgat gttctcgcgc acgacgatcg
     5221 cgtcgtcgac gaggataccg atcgcgagcg agaggccgag cagcgacatc acgttgagcg
     5281 tgaagccgaa cgccagcacc gcgacgaacg acgcgatcac cgacacgggc agcgcgaggc
     5341 cggtgatcac cgtcgagcgc cacgacttga ggaagatgaa cacgacgaga atcgtcagca
     5401 gtgcgccttc gaacagcgac gtctgcacgt ccttgaccga cgcgtcgacg cgcgcacccg
     5461 agtctctgac gatctcgagc ttcgtgccct tgagctgtgg atcttgctgt agctcggcga
     5521 tccgtttgag cacgcgctga ctgacgtcgg tcgtgctgta gccgagcgcc ttcttgatat
     5581 cgatcccgac ggcgggcgtc ttgttgaaga acgcggccgt gagcggctcc tcctggccgt
     5641 cgcgcacctt ggcgacgtca ccgagtcgca cgacgcggtc gccgctgcgt ccgacgacga
     5701 gctgctcgaa ctcggaaggc cccgacaatc gaccgcgcag tcgaatcgtg cggtcctcga
     5761 gcttgccgct cagcttgccg acggggacgg cgaggttttc cgtctgcagc gtctggacga
     5821 cttgcgagat cgacacaccc gacgcgcgca gcgcttccgg ctgcacctcg accgtcagct
     5881 cgcgctcgac accgccgaca atgtcgacgc tcgcgacgcc ctgcagcgag gtcagctcgc
     5941 ggcgaatccg ttggtccgcg agctcggtga gcctgaccgg cgcgacggtt gtcgacgtca
     6001 gagtcagcga gatgatcgga aagtccgacg acgcgaaccg cgtcaggatc ggctcctcca
     6061 tctcgatcgg cagatccgcg cgagactccg aaatcttgtc gcggatgtcc tgggtcgcct
     6121 gctggatgtc tttctcgaag acgaactgga cgaggatgtt cgcgtagccg tcgaccgccg
     6181 tcgaattgat cgtgtcgaca cccgagatcg agcggaacga ttcttcgagc ggattgatca
     6241 gctcacgctc gacgacaccg ggcgatgcac cgggatagac gatcgcgacg ttgacgaccg
     6301 gcgcggtgac ctcgggaaac tcgtcggtat gaaggcgcac gagcgcgatc aagccgaaca
     6361 cgacgagcgc caccatcacg accgtcgtga tgatcggttt cttgatcgcg tagttcgaga
     6421 tgaacattac ttgcctgcgc ttcccgcggc cggcgccttc ttgtcggccg gcttctcgtc
     6481 cggcgtcgtc ggcttcgcag ctggtgcttg cggtttctca tcgggcgctg cggatttctc
     6541 atcgggcgct gccggtttgt cctcggtctt cgctccctgc tgcggcagct cgaccttcgc
     6601 gcctggcccg acgttcttct gcgcacgcgc cagcacgagc acgtctcccg gtgcgagccc
     6661 actcgtcacc tcgacgactt ccgcacgctc gtcacgcacg ccgagcgcga cgatcacgcg
     6721 ttcgacgaca ctgttcttca cgcgcagcac ggtcggctga tcacctgccg aatcgatcgc
     6781 accaaccggc gccgtcaacg cttcgcgccg ctcgactgcg attcgacctt cggcatagag
     6841 gccggcgatc aacttgccgc ccgggttcgg aatgtcgacg agcacctgga tctggcgcgt
     6901 gaccggatca gcagccggcg cgatgcgctg gatctttccg gtgaacagct gaccgggata
     6961 gccgcgcacc tcgaagtcca ctgccttgcc gagcgcgatc accgacaagt cctccgatgc
     7021 gacgctggcg tcgagccgca tcgtcgacgg atcgatcacc tcgtagagct cgctacccgg
     7081 cgacacgacg tcgccgacac tgatcgcacg ccgcgcgacg acgcctgcga gcggcgcctt
     7141 cacggtggca tcgccgagct gcgactgcga ggacgcgacc tgtgcgcgcg cctgcgtgac
     7201 cgccgccgtg gctgcgctga gctcgctctt cgcgcggtcg agctcgcgcg cggctagtgc
     7261 gccgcccttc acgagcgcct cggtgcgctg cacttcgcgt agcgcgagat cgtactgcgc
     7321 ctgcgccgac tcgagcccgc tctttgccga cgacgcgacg tcgccgagcg ccttcgcttc
     7381 gatgcgtgcg agcaggtcgc ccttcttcac cggctggccg agctccgggc cgatcgcgac
     7441 gaccgacccc tgcgtctccg cgcgaacgac ggcacgcgac gcggcctgca acgtgcccga
     7501 gatgcgcggg cccgtttgca gctcaccgcg cgcaaggacg acgatgtctt cgggcgcgag
     7561 cttgatcgtc ggcacttcgt tcgtgctctt gggttgctct tgtgcgccct tcttacaagc
     7621 ggcaagcgcg atgacgatga cgatgattgt gaagagccgc atacgtctcc tagaaaacca
     7681 gctgtaggcc aagcggaagt gcgggaagca gctcctcgcg gaaccgcgcg acgcgaagat
     7741 ctcgagcagc gcgcgcctgg acgagcagtg cttgttcgag ttggacgcgc gcgtcggtga
     7801 tttcgaggtg cgtcgatgcg ccctgcgaga accgcagctc ggcgatctcg tacgcgcgct
     7861 gcgcttgttt cacggtgcgg atgctgttct cgagctgcgt ctggctcgct tcgacgctcg
     7921 cgcgcgcttg cgcggtctcg acattcgaga ctttgctgga gagctggagc tgctgcttcg
     7981 ctgccgcgag ctccgcttgc gtcgtgcgca cctgagcctc gcgcctcagg ccgtcgaaga
     8041 ccggcagcga cagcgtgacg ccgagcgtcc agttcgttcg ccagtccgga tggaacggcg
     8101 tgttgtcgta gtcgacaaag ccgaggtcgg tgcccgcgga gaggctcggc caatactgcg
     8161 acttcgcgag cttcaacgcc gattcgcgaa tgccgacgct ctcctttgcc tgtgcgactg
     8221 cgatccggtt ggtgctcgtg atgccagctg ctttgcgcgc ggtcgcgagc aattgctcga
     8281 ggtcgttcga atcgagcgtc gacgtcagct ggagctgccg atcgagtggc aggccgacga
     8341 gccggcgcag cgacacgagc gtgacgtcgc gctggacctt gaactgcacg acgagcgtgc
     8401 gctggttgtc gcgcacgacc tcggcgcgaa cgagatcgaa ctcgggccgc gtgccttgtt
     8461 tgaagccgag ctcggtctcc tggtacgtct gctcggcctg ccgcaaggtt tcctcgccga
     8521 tcacgacctg acgctcggcg agcaccgcat cgaagtacgc ctgcgcgacc tgcagcgcga
     8581 cctgcgcgcg catcgaattg acacctagct cggcgtgacg cacgccgccc tttgcgacat
     8641 cgactgcatt cttcgtacga aagccatcaa agatcggctg cgtcacgttg aggttgacgc
     8701 gccagttgtt gcgctggcca aacggaagct tgacgtcgct cccgcccgtc atcgggccga
     8761 agttgatgtc gtcgaactca ctcgcgagcg tgcgctgata tgcgagcgag ctgttgaccg
     8821 tcggcaagta acccgcgcgc gccgacgtca cttgtgcgtc ggctcggtcg acatcggcat
     8881 ttgcgatcgc gaccgactcc gccgttcgct cggcggcacc gaccgcctct tcgaatgaca
     8941 gcgccgtcgg cgattccggc gtggccgatg gcggctgcgc agcagcggac ccggccagcg
     9001 tgaggctgca caggccggcg acgaaggaca aacgaagcat catggggccc tttgcaaaat
     9061 gcaggcacga gcttccctcg aaaccgcgcg ccagacgcgt ccgaagcgcg tcaaacctga
     9121 cagacgctgc tctagaaaca gcgttcctgt agggaatccg acagccttat gtaaggagca
     9181 cgatcaggat gccgatcgtc gacacgatga tcgcagcggc gagaatcacg aaaatgcgac
     9241 gtccgcgacc gctgctcgtc ggccgcggca ccagctgtga ctgcggcggc atcgagccct
     9301 gcggatacgt cgtcggcgga atcgaaccgg gaatcggcgt cgtgtacggc ggaacgctcg
     9361 gtgggtgctg ctgtgac
//
LOCUS       DASUFX010000329         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1718602, whole genome shotgun sequence.
ACCESSION   DASUFX010000329 DASUFX010000000
VERSION     DASUFX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718602"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VFV99_13245"
     CDS             complement(<1..1388)
                     /locus_tag="VFV99_13245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060325.1"
                     /translation="MVHAGDAPIDRYVVLEHLSEGGMGAIYVGKKLGAGGFEMEVVLK
                     QLLPEFTQQEEFIDLFLREAKLSATLDHANIVHTIDLVTAGGEYFIVMEYLPGGDLRT
                     LLKKAKRRGKRFSPKAAIYIARELLSALSYAHSKRDFDGNPLKLIHRDVSPSNVLLSY
                     AGEIKLTDFGIAKAATHTSLFYKVKGKIGYMSPEQAKSEPLDHRSDLYSLAVCMYEIL
                     TGERLFVHAGLTTSADEIYSQPVPQLSRKVPGLTPDLDAIMRKALSIDPDKRYQTAGE
                     FQEALMRCAHRNGLLMSAPELSRELLDACGPTDQWRGDDDDDDDLSVPRAGTEVYDPG
                     DDEDDDDDSALEIKPSGPLSIHSIAARARSSPSGAKSPPLNTRSPPPLRPKTEVGKFA
                     GVELTSIINMMDVDDRLGSQPLVDLDSPPMQRPMERVDSDPSLMSFVQSLPPARGGGY
                     EAPRRSGFDESSP"
     gene            1413..1856
                     /locus_tag="VFV99_13250"
     CDS             1413..1856
                     /locus_tag="VFV99_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060326.1"
                     /translation="MAGEDRYRLAPVRDERARNERVRRGDLAVAVGDARETAARLDAA
                     RARTQAARDALTAAVAARDSLTRTDTTPAQLVLADRFIARRRGELDRILGEELRVEAA
                     HESRLDSLDVARQRLVRARADREVIERHFATWRHTQQKLAERRED"
     gene            1937..2230
                     /locus_tag="VFV99_13255"
     CDS             1937..2230
                     /locus_tag="VFV99_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060327.1"
                     /translation="MRILLATLLLFPAIAFADDAKPQAPVQEAQGNPDDCARARKLGQ
                     TCVLTIGEEDIEGGVSRPDGIGFSARDWAYMNSLIHIRRDFIPEIVKSAEDID"
     gene            complement(2231..2482)
                     /locus_tag="VFV99_13260"
     CDS             complement(2231..2482)
                     /locus_tag="VFV99_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060328.1"
                     /translation="MRIALVLALLAGCTQPRSKTCVDICSRESDCITATKADIPFDEK
                     ECVSACEVLRADQQNLAKVESHRECVMKNASCKDVLECP"
BASE COUNT          443 a          864 c          822 g          437 t
ORIGIN      
        1 ggcgacgact cgtcgaaccc cgaacgtcgc ggcgcttcgt agccgccgcc acgcgcgggc
       61 ggcagcgact gcacgaacga catcagcgat ggatccgaat cgacgcgctc catcggacgc
      121 tgcatcggtg gcgagtcgag atcgacgagc ggttgcgagc cgagccgatc gtcgacatcc
      181 atcatgttga tgatcgacgt cagctcgacg cccgcgaact tgccgacctc ggtcttcggc
      241 cgtagcggcg gcggtgatct cgtgttgagc ggtggtgact tcgcaccgct cggcgacgat
      301 cgcgcgcggg ctgcgatgct gtggatcgac agcggtccgg acggcttgat ctcgagcgcg
      361 ctgtcatcat cgtcgtcctc gtcgtcgccc gggtcataga cctcggtgcc ggcacgcggg
      421 acggacagat cgtcgtcgtc atcgtcatca ccacgccact gatcggtcgg gccgcacgcg
      481 tcgagcagct cacgggaaag ctcgggcgcc gacatcaaga ggccgttgcg atgcgcgcac
      541 cgcatcagcg cttcctggaa ctcgccggcg gtctggtagc gcttgtcggg atcgatcgac
      601 agcgccttcc gcatgatcgc gtcgagatcg ggcgtcaggc ctggcacctt cctcgacagc
      661 tgcggcaccg gttgcgagta gatctcgtcg gcgctcgtcg tcaggccggc gtgcacgaac
      721 aaccgctcgc cggtcaggat ctcgtacatg cacaccgcga gcgagtagag gtcgctgcga
      781 tgatcgagcg gctcgctctt cgcctgctcg ggcgacatgt agccgatctt tcccttcacc
      841 ttgtagaaca gcgacgtgtg cgtcgcggcc ttcgcgatac cgaagtcggt cagcttgatc
      901 tcgcccgcat acgagagcag tacgttcgac ggcgacacgt cgcgatggat gagcttgagc
      961 ggattgccat cgaagtcgcg cttcgagtgc gcgtacgaca gcgccgatag cagctcgcga
     1021 gcgatgtaga tcgccgcctt cggcgagaac cgcttgccgc ggcgcttcgc cttcttgagc
     1081 agcgtgcgca agtcgccgcc cggcaggtac tccatcacga tgaagtactc gccacccgcg
     1141 gtcacgagat cgatcgtgtg gacgatgttc gcgtggtcga gcgtcgccga cagcttcgcc
     1201 tcgcgcagga acaggtcgat gaactcttcc tgctgcgtga actcgggcaa cagctgcttg
     1261 aggacgacct ccatctcgaa gccgcccgcg ccgagcttct tgcccacata gatcgcaccc
     1321 atgccgccct cggagaggtg ctcgagcacg acgtagcggt cgatcggcgc gtcgcccgcg
     1381 tgaaccattc gccggcagtg taacattgcg ttgtggctgg cgaggaccgc taccggctgg
     1441 ctcccgtgcg cgacgaacgc gcgcgaaacg agcgggtccg ccgcggagat ctcgcagttg
     1501 cggtcggcga tgcacgcgaa accgctgccc gactcgatgc ggcacgcgca cgtacgcagg
     1561 ccgcgcgcga cgcactgacg gccgcagtcg ccgcacgcga ttcgctcacg cgcaccgaca
     1621 cgacacccgc ccagctcgtg ctcgccgatc gcttcatcgc gcgccgtcgc ggcgagctcg
     1681 accgcatcct cggcgaggag ctacgcgtgg aagccgcgca cgaatcccgt ctcgactcgc
     1741 tcgacgtagc gcggcaacgg ctcgtccgtg cgcgcgcgga ccgcgaggtg atcgaacgcc
     1801 acttcgctac ctggcgtcac acccagcaaa agctcgccga acgccgcgaa gactagtcgg
     1861 acatccggct accgacttcg gacgtcaggc tcggggtcaa ttgcaaccgc gagggttcga
     1921 tgcgtgtctc gccgtcatgc ggatcctcct cgcgaccctg ctcctgttcc ccgcgattgc
     1981 ctttgccgac gacgccaagc cccaggcgcc ggtccaagaa gcgcaaggca accccgatga
     2041 ctgcgcgcgt gcgcgcaagc tcggccagac gtgtgtgctc acgatcggcg aagaggacat
     2101 cgaaggcggc gtgtccaggc ccgacggcat cggcttctcg gcgcgcgact gggcgtacat
     2161 gaacagcctg attcacatcc ggcgcgactt cattcccgag atcgtcaagt cggccgaaga
     2221 tatcgactga ctacggacac tcgagaacgt ctttgcacga ggcgttcttc atgacgcact
     2281 cgcggtgcga ttcgaccttc gccaggttct gctgatccgc gcgcagcacc tcgcacgccg
     2341 acacgcattc cttctcgtcg aacgggatgt cggctttcgt cgcggtgatg cagtcggact
     2401 cgcgcgagca gatgtcgacg cacgtcttgc tgcgcggctg cgtacagccg gcgagcaggg
     2461 cgaggacgag cgcgatgcgc atggtcatag tctacgcgaa cgccttcgcg atcgcgcgca
     2521 tcgtcatgta gatcaggaac gcgaggagaa tgaacaacgc agcgac
//
LOCUS       DASUFX010000330         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_468189,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000330 DASUFX010000000
VERSION     DASUFX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468189"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFV99_13265"
     CDS             complement(<1..469)
                     /locus_tag="VFV99_13265"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX5060329.1"
                     /translation="MAQPSNPPPQYGYQQQQPGYQQPGYQQPPPPTQTQPPPTQPAVT
                     QPPPPTQPEPPPPPPPAEPAYYDTPGSVNVNVSFQGDAVWNLDVFFDQLDPYGTWYDD
                     QTYGWVFVPSTANYVPYSNGYWKYTDYGLLWVSNDPFGWATDHYGRWVYLNRWV"
     gene            594..1967
                     /locus_tag="VFV99_13270"
     CDS             594..1967
                     /locus_tag="VFV99_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060330.1"
                     /translation="MRVLRLTEVVRGRTSIAASCSLDDLRFHATVWYQDVDLDELARV
                     HGAEVLDRIALHVALFQLNAVASLRPDAIELGVYSRFLTPALVRLWQTVFRNVWAQWR
                     WQHDLRDYVPEFANAPVESEAAPIEAPNGDTELLAFCGGGKDSLVALKLLERARLPFA
                     TLGYAHSIYGGAAEQHALLDRLGGMTARVRAERQWIVDDFLDSPVAALRPDLGVKSVL
                     AAETPASVFAALPIALARGYRGLVVAHEASANAANLDWNGEPVNHQWGKSWDAEQLLD
                     RYVREQLVSNVRYFSLLAPVNDEVIFELLARDAELAPLTHSCNVKKPWCGECAKCAYV
                     WLQFAAHLPADIVDKTFGANFGERAVNLQHFHQLLGLAEHTPFECVGSAAEARLALTL
                     ARDRGVLGTRLSALVDELGTIDIAAHARSLVELGSNHGMPGHVAAVVMPLLSDAARSA
                     RARLLGE"
     gene            complement(1953..>2209)
                     /locus_tag="VFV99_13275"
     CDS             complement(1953..>2209)
                     /locus_tag="VFV99_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060331.1"
                     /translation="ALGQTYEGKFAPPAHMAKQMGINEVRLTIDYLGANLKVRMRLDR
                     KGLRHDRAIDQVFELPRLRAASQPEVNATIKAMLDQLLAQ"
BASE COUNT          338 a          698 c          771 g          402 t
ORIGIN      
        1 acacccagcg gttgagatag acccagcggc cgtagtgatc ggtcgcccag ccgaacggat
       61 cgttcgagac ccagagcaga ccgtagtcgg tgtacttcca gtagccgttc gaatacggca
      121 cgtagttcgc cgtcgacggc acgaacaccc agccgtacgt ctgatcgtcg taccaggtgc
      181 cgtacggatc gagctgatcg aagaagacgt cgaggttcca gaccgcgtcg ccttggaagc
      241 tgacgttgac gttgaccgag ccgggcgtgt cgtaatacgc gggctcggcc ggcgggggtg
      301 gcggaggagg ttcgggttgc gtgggtggtg gcggttgcgt caccgcgggc tgcgtcggcg
      361 gtggctgcgt ttgggtcggc ggaggcggct gctgatagcc cggctgttga tagcccggct
      421 gttgctgctg atagccgtat tgaggcggcg ggttactcgg ttgagccacg caacccgcga
      481 acaacagacc agccaacgct ttggatagac gcataggtac ctttcgacgg ctgacgccgg
      541 acccgaattc accgccacga taccggtttt gtgaggatcg cgtacggttg ggcatgcgcg
      601 tcttgcggct gaccgaggtc gtacgaggtc gcacaagcat cgcagcgagc tgttcgctcg
      661 atgacctgcg cttccacgcg acggtctggt atcaggatgt cgatctcgac gagctcgcgc
      721 gcgtccacgg tgccgaggtt ctcgatcgca tcgcgctaca tgtcgcgttg ttccagctca
      781 acgccgtcgc gagcctcagg ccggatgcga tcgagctcgg cgtctactcg cggtttctga
      841 cgcccgcgct ggtgcgcctg tggcagaccg tgtttcgcaa tgtgtgggcg cagtggcgct
      901 ggcagcacga cctgcgcgac tacgtgccag agtttgccaa cgcgcccgtc gagagcgagg
      961 ctgcgccgat cgaagcgccg aacggtgaca ccgagctgct cgcgttctgc ggcggcggga
     1021 aggacagcct ggtcgcgctc aagctgctcg agcgtgcgcg attgccgttc gcgacgctcg
     1081 gctacgccca ctcgatctat ggcggtgccg ccgagcaaca cgcgctgctc gatcgcctcg
     1141 gcggcatgac tgctcgcgtg cgcgcggagc ggcaatggat cgtcgacgat tttctcgact
     1201 caccggtagc cgcgttgcgt cccgacctcg gcgtgaagtc agtgctcgcg gcggagacgc
     1261 cagcgtcggt gttcgcggcg ctgccgatcg cgctcgctcg tggttatcgc gggctcgtcg
     1321 tcgcgcacga ggcgagtgcg aacgccgcga acctcgactg gaacggcgaa ccggtcaatc
     1381 accagtgggg caagagctgg gacgccgagc agctgctcga tcgctacgtg cgcgagcagc
     1441 tcgtcagcaa cgtgcgctac ttcagcctgc tcgcgcctgt gaacgacgaa gtgatcttcg
     1501 agctgctcgc gcgcgatgcc gagctcgcgc cgctcacgca ctcgtgcaac gtgaagaagc
     1561 cgtggtgcgg cgagtgcgcg aagtgcgctt acgtctggct gcagtttgcg gcgcacctgc
     1621 ccgccgacat cgtcgacaag acgttcggcg cgaacttcgg cgagcgcgcg gtcaacctcc
     1681 agcactttca tcagctgctc ggcctcgccg agcacacgcc gttcgagtgc gtggggagcg
     1741 ccgctgaagc gcggctcgcg ctcacgcttg cccgcgatcg gggagtgctc ggcacgcggc
     1801 tcagcgcgct cgtcgacgag ctcggcacca tcgatatcgc cgcccacgcg cgttcgctcg
     1861 tcgagctcgg ctcgaaccac ggaatgcctg gtcacgtcgc ggcggtggtg atgccgctgc
     1921 tctcggatgc ggcgcggtcg gcgcgagcgc ggttactggg cgagtagctg gtcgagcatc
     1981 gccttgatcg tcgcgttgac ctcgggctgc gaggcggcgc gcaggcgcgg cagctcgaac
     2041 acctgatcga tcgcgcggtc gtggcgcagg cctttgcgat cgagacgcat gcgaactttg
     2101 aggttcgcgc cgaggtagtc gatcgtcaga cgcacctcgt tgatgcccat ctgcttcgcc
     2161 atgtgcgcgg gcggcgcgaa cttgccctcg taggtctggc cgagcgcgg
//
LOCUS       DASUFX010000331         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1580086, whole genome shotgun sequence.
ACCESSION   DASUFX010000331 DASUFX010000000
VERSION     DASUFX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580086"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFV99_13280"
     CDS             <1..423
                     /locus_tag="VFV99_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060332.1"
                     /translation="GDPAHSAYFINDNGNASGAGTVTFTGNWTAYPGCGAQCFYGDDH
                     GSSTTGATATFQFTGTQIALFSVCDAGNGLAGFSLDGGAETMRDYYASIRQGEQAIYT
                     SPVLPFGPHELRVRVTGQHASGSSGAAISIDRAEVFTQ"
     gene            423..839
                     /locus_tag="VFV99_13285"
     CDS             423..839
                     /locus_tag="VFV99_13285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5060333.1"
                     /translation="MPRLTLTELEAVLDEHSPRWRKHARILGLGVQDIHVTMPLGAEL
                     VRAGGTFSGPALMALADRAAYYLTLALVGPVPDAVTSNLAIHFLVRPQPGDITATATV
                     LRIGSRLVVSSVEMHAAEQLVAHATVTYALPQAYTS"
     gene            complement(936..2231)
                     /locus_tag="VFV99_13290"
     CDS             complement(936..2231)
                     /locus_tag="VFV99_13290"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEX5060334.1"
                     /translation="MNAAVRASMLLLAVGCADATVPDRVSPDAGTIEPTPDADTFHTS
                     LVNAGQVCKLLSNRNASDPTGNDVQHAANVLGADLGIPVVTNDKLYIFFGDTIGYDTI
                     WGATESHPDSVGYGLDSATAIAAQPDLLCNRLGIVTLPVSQSIGPGNNSAIKADFAGA
                     AMLAPSGHSLPEYIHNPSGPSGSTSFPHLPGDFEVPSGAFAVNGSIYVFYTTVVGPTN
                     VAMKGSYLAKWVSPTTTGPFGYQILYQVDERFDANGALHGHFINIAAEVVGDYVYLFG
                     TGDYRRSKIYLARKPVAMIDQPGGFEELGEIVTTPGYGEISVRYFPSLEHWMLLAEEL
                     TPSSNHIVAYFAANPEGPWSAPIVVHDMASSSFRSQYCCTSEDNCIGQQMFNCNRTGF
                     YGTYLFPAIDDNATSFKVTYTMSSFSPYNVALFEASFAK"
     gene            complement(2228..3463)
                     /locus_tag="VFV99_13295"
     CDS             complement(2228..3463)
                     /locus_tag="VFV99_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX5060335.1"
                     /translation="MKRYRHAFAFALSATLVGCVGNAPGSGASSDTGSGSTSDDSGAG
                     SGDEDSSSGGEGGGDGGGSGATETIAGETHWKVLNHDYQAQTTGYWCGPAASRIALST
                     RVAPPTQQALANELGTTTNGTDWIGQVTAALNNHLGARYATVEMPNDPPTPAQRDRLW
                     ADVMLSIDAGHGMVANIVAPPNNHPPGYPNETIYHYISLIGYNPDNLQVYVGDSANFG
                     GNNHYWLTLDQLSTLIPPKGYSTYRCGVGRTMGEIDARYQGFGGCTGFLGAALTDETT
                     APDNTGKYNVFRNGSIYWSPTTGAFEVHGAIRDKWKELGWEAGALGYPVSNETKTPDN
                     VGRFSVFQRGSIYWTPATGAHEVHGAIRDKYKEVGWEAGALGYPTSDEYAVSGGRRSD
                     FQHGSITWDSATNTCTVTQ"
     gene            complement(3472..>4520)
                     /locus_tag="VFV99_13300"
     CDS             complement(3472..>4520)
                     /locus_tag="VFV99_13300"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060336.1"
                     /translation="GLRTGYLLAGIANDETNLAMCWSEATWACKGPASPDCGGGPIIA
                     GSADGPCGNQQGGLGMFQFDAGTYQQTIDRYGTAILTIDGQIGSAIDYATWMVKVSAY
                     TTNAETDAKARAWLNNFDPNNGALRDQWIKTVVRYYNGCQPGWSCWNARYQTYSDGYY
                     AAINEPGGVGFWGSIGTSCNGSPATVGKIDEKYRALGGCGSFLGKPITGETGAPDGVG
                     RYSVFENGSIYWTPSTGAFEVHGQIRDKWKELGWEAGLLRYPITDETQTPDGVGRFNV
                     FEGGSIYWSPSTGPHEVHGVIRDKYKAEGWEAGALGYPTSDEYAVTDGRRSDFQHGYI
                     TFSSSTNTATVTYQ"
BASE COUNT          747 a         1474 c         1459 g          840 t
ORIGIN      
        1 ggcgatccgg ctcacagcgc gtacttcatc aacgacaacg gcaacgcgag cggcgccggc
       61 accgtgacgt tcaccggcaa ctggaccgca tatccaggct gtggtgcgca gtgcttctac
      121 ggtgatgatc acggttcgag caccaccggc gcgaccgcga cgttccagtt cacgggtaca
      181 cagatcgcgc tgttctcggt gtgcgatgcc ggcaacggcc tcgctgggtt ttctctcgac
      241 ggtggcgccg aaacgatgcg cgattactac gcgtcgatcc ggcaaggcga gcaggcgatc
      301 tacacgagtc cggtgctgcc attcggtccg cacgagctgc gcgtgcgcgt gacgggtcag
      361 cacgcctctg gctcgagcgg cgccgcgatc agcatcgatc gcgccgaagt gttcacgcag
      421 tgatgccgcg gctcacgctc accgagctcg aagcagtact cgacgagcac tcgccgcgct
      481 ggcggaagca tgcgcgcatt ctcggcctcg gcgtccagga catccacgtg acgatgccgc
      541 ttggcgccga gctcgtgcgc gccggcggta ccttctccgg accagcgctg atggcacttg
      601 ccgatcgcgc ggcctactac ctcacgctcg ctctcgtcgg gccggtgccg gacgcggtga
      661 cgtcgaacct cgcgattcac tttctcgtgc gcccgcaacc gggcgacatc accgcgacgg
      721 cgacggtgct acgcatcggc agccgcctcg tcgtgtcgtc ggtcgagatg cacgcagccg
      781 agcagctcgt cgcgcacgcg acggtcacct atgctttgcc gcaggcttat acgagctgag
      841 cacatactcg ttgccgcgtg cgcgaatcac tcgccgcagc agtgcatgac gcagataccc
      901 gggtgacatg agcagaatgt ccgccatgga gacctctatt tcgcaaacga cgcctcgaag
      961 agcgccacgt tgtacgggga gaacgaggac atcgtgtacg tgaccttgaa cgaggtcgcg
     1021 ttgtcatcga tggccgggaa cagataggtg ccgtagaagc cggtgcggtt gcagttgaac
     1081 atctgctggc cgatgcagtt gtcctcgctg gtgcagcagt actgcgagcg gaagctcgag
     1141 ctcgccatgt cgtgcacgac gatcggcgca ctccacggcc cctcgggatt cgcggcgaag
     1201 tacgcgacga tgtgattcga cgacggcgtc agctcctcgg cgagcagcat ccagtgctcg
     1261 agcgacggaa agtagcgcac cgaaatctcg ccgtagcctg gcgtggtcac gatctcgccg
     1321 agctcctcga agccacccgg ctgatcgatc atcgcgaccg gcttgcgcgc gagatagatc
     1381 ttgctgcgcc ggtagtcgcc ggtgccgaac aggtagacgt aatcgccgac gacctccgcc
     1441 gcgatgttga tgaagtgccc gtgcagcgcg ccgttcgcgt cgaagcgctc gtcgacctga
     1501 tagaggatct ggtagccgaa tggtccggtc gtcgtcggtg acacccactt cgcgagatag
     1561 ctgcctttca tcgcgacgtt cgtcgggcca acgacggtcg tgtagaacac gtaaatcgag
     1621 ccgttgaccg cgaacgcgcc cgagggcact tcgaagtcac ccggcaagtg cgggaacgac
     1681 gtgctgccac tcggccccga cgggttgtgg atgtactcgg gcagcgagtg gcccgatggc
     1741 gcgagcatcg cagcgcctgc gaagtctgct ttgatcgccg agttgtttcc gggcccgatg
     1801 ctctgtgaga ccggcagcgt cacgatcccg aggcgattgc acaggaggtc cggctgcgcg
     1861 gcgatcgcgg tcgccgaatc aagcccgtag ccaaccgaat caggatgcga ctcggtcgct
     1921 ccccagatcg tgtcgtagcc gatcgtgtcg ccaaagaaga tgtacagctt gtcgttcgtg
     1981 acgaccggaa tgccgaggtc ggcgccgagc acgtttgcag catgctggac gtcgttcccc
     2041 gtcggatccg atgcgttgcg attcgacagc agcttgcaca cctgcccagc gttcacgagc
     2101 gacgtgtgaa aggtgtccgc gtccggtgtc ggctcgatcg tgcccgcatc gggcgagacc
     2161 cgatcgggca ctgttgcatc cgcgcagcca actgcgagca acaacatcga ggcgcgcacc
     2221 gccgcgttca ctgggtgacc gtgcaggtgt tggtggccga gtcccaggtg atcgagccgt
     2281 gctggaagtc ggagcggcga ccaccggaca ccgcgtactc gtcggaggtc ggatagccga
     2341 gcgcgcccgc ttcccacccg acctctttgt atttgtcgcg gatcgcaccg tgcacctcgt
     2401 gcgcgccggt cgccggcgtc cagtagatcg agccgcgctg gaacacgctg aagcggccga
     2461 cgttgtcagg cgtcttcgtc tcgttcgaca ccggatagcc gagcgcgccc gcttcccaac
     2521 cgagctcctt ccacttgtcg cggatcgcgc cgtgcacttc gaacgcgccg gtggtcggcg
     2581 accagtagat cgagccattg cggaagacgt tgtacttgcc ggtgttgtcg ggcgccgtcg
     2641 tctcgtcggt gagcgccgcg ccgaggaatc cagtgcagcc gccaaagccc tggtagcgcg
     2701 cgtcgatctc gcccatcgtg cgaccaacgc cgcagcgata cgtcgagtag ccctttggtg
     2761 gaatcaacgt cgagagctga tcgagggtga gccaatagtg gttgttgccg ccgaagttcg
     2821 ccgagtcgcc gacgtagacc tgcaggttgt cggggttgta gccaatcagc gagatgtagt
     2881 gatagatcgt ctcgttcgga taaccgggcg gatggttgtt gggcggcgcc acgatgttcg
     2941 cgaccatgcc gtggccggcg tcgatcgaga gcatcacgtc ggcccagagt cgatcgcgct
     3001 gcgctggcgt cggcggatcg ttcggcatct cgacggtcgc gtagcgcgcg ccgagatgat
     3061 tgttgagcgc ggccgtgacc tgcccgatcc agtcggtgcc gttcgtcgtc gtgccgagct
     3121 cgttcgcgag cgcctgctga gtcggcggcg cgacgcgcgt cgatagcgcg atgcggctcg
     3181 ccgccggacc gcaccagtag ccggtcgttt gcgcctgata gtcgtggttc aagaccttcc
     3241 agtgggtctc gcccgcgatg gtttccgtcg cacccgagcc accaccatca ccaccgcctt
     3301 cgccacccga gctcgagtct tcgtcgccgg atcctgcacc gctgtcgtcg ctcgtcgatc
     3361 cactgccggt atcgctgctg gcaccgctgc ccggcgcgtt gccaacgcaa ccgacgagag
     3421 tggcggacag agcgaacgca aacgcatgtc gatatcgctt catgactctc ctcactgata
     3481 ggtgacggtc gcggtgttgg tgctggagct gaacgtgatg tagccgtgct ggaagtcgct
     3541 gcgccggccg tcggtgacgg cgtattcgtc agacgtcgga tagccgagtg cgccggcttc
     3601 ccacccttcc gctttgtatt tgtcgcgaat cacgccgtgg acttcatgcg ggcccgtcga
     3661 cggcgaccag tagatcgagc caccctcgaa cacgttgaag cgcccgacac cgtcaggcgt
     3721 ctgcgtctcg tcggtgatcg ggtagcgcaa caggcccgct tcccagccga gctccttcca
     3781 cttgtcgcgg atctggccgt gtacctcgaa tgcgcccgtc gacggcgtcc agtagatcga
     3841 gccgttctcg aacacgctgt agcgaccgac accgtcgggg gcgccggtct cccccgtgat
     3901 cggcttgccg aggaacgaac cgcaaccgcc gagcgcgcgg tacttctcgt cgatcttgcc
     3961 gaccgtcgcc ggcgagccgt tgcagctcgt gccgatcgat ccccagaagc cgacgccgcc
     4021 gggttcattg atagcggcgt agtacccgtc gctgtacgtc tggtagcgcg cgttccagca
     4081 gctccagccc ggctggcagc cgttgtagta gcggacgaca gtcttgatcc attgatcgcg
     4141 cagcgcgccg ttgttcggat cgaagttgtt gagccacgcg cgcgccttcg cgtcggtctc
     4201 ggcgttcgtc gtgtacgcgg agaccttgac catccatgtc gcgtagtcga tcgcgctgcc
     4261 gatctgaccg tcgatcgtga ggatcgccgt cccgtagcga tcgatcgttt gctgatacgt
     4321 gccggcgtcg aactggaaca tgccgagacc accctgttgg tttccgcacg gcccgtccgc
     4381 cgaaccggcg atgatcggac cgccgccgca atcgggcgac gccggtccct tacatgccca
     4441 ggtcgcttcc gaccagcaca tcgcgaggtt ggtctcgtcg ttcgcgatac ctgcgagcag
     4501 ataaccagtg cgcagaccgc
//
LOCUS       DASUFX010000332         4813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_190251,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000332 DASUFX010000000
VERSION     DASUFX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190251"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="VFV99_13305"
     CDS             complement(<1..1870)
                     /locus_tag="VFV99_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5060337.1"
                     /translation="MLPRITISTVAASALVVVPALAQDVVDGASPQPATATDAEGEET
                     IEVVDKAPPGARAELTKEALERDEHDDLHKVLRGIAGVYLRDEDGYGLRPNIGMRGAA
                     ADRSAKITLMEDGVLIAPAPYTAPAAYYVPLVTRMSRIEVTKGPSAIRFGPATVGGAL
                     DLIGEPFPAERSGYVDVAGGSDLYGKLHARAAERRERWGVMAEYVHLRSDGFKDLDGG
                     GPTGFDKNDVQLTTRFMSEPTAETYHQLDLRVGYGDETSHETYTGLTDEDFAAAPQRR
                     YVASQLDEMNWGHWRFRATHRVDLGVATRVETVAYRHKFHRVWGKVDGLIGQRDLYAL
                     LAEPTAGANQVYYAILTGAADSQSPEEPLVLGTNDRTFTSQGAQTTVSLERATGPVKH
                     HVDAGARIHFDRADRRRYEDTYDMTMATLVRTARSRATVLDTRAETIALALFAQDKLH
                     WKRFELAAGTRIELIDYRFADWLTMAQHDGSYAVVIPGAGAEYHVTGNAGVLAGVHRG
                     FVPVAPSAGSGVLPESSINYEAGARWRDERIAADVIGFFSDYSNLKGSCTLSAGCTET
                     QEGAEYNGGAVFTWGAEAQLSGEVPLVRELVLPISAAYTWTRSRFRHAFDSEFAGWG"
     gene            complement(1848..3122)
                     /locus_tag="VFV99_13310"
     CDS             complement(1848..3122)
                     /locus_tag="VFV99_13310"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX5060338.1"
                     /translation="MSGILRGQILAGRRAGLALAGLAALGPAALGPAAGLAALATTAS
                     ALAGCGSSDPAKADAIPDNFDRNALLAHLGEHVLLPIQATFDARANALPAAIAAYCDA
                     LDAGTPGTTLESARAAWREAIDAWQHAEALLVGPAAMDNKALRDRIYAWPLLAPCDVD
                     RDVASRWANPASYDVSTKLQRQRSLLAIEYLLFPQTDAHNCAITPPGWDALGADLLRA
                     RCRLAEVLATDVAAAATTLHTAWRADGGNYVGELARAGMGTSIPSAHAGVNLVSDSFF
                     YVDRMVKDMKLAEAAGIAVNACGTVQEPCIREVELRFADRATTAIRINLATLREAITG
                     TTPAADGPGFDDFLIAIGQPEVATRVTAALDAAIAKADALPESFLGALTNDYAAIVEA
                     HAATKEFTNDLKSQFLTLLALDIPDDVASDND"
     gene            complement(3223..4599)
                     /locus_tag="VFV99_13315"
     CDS             complement(3223..4599)
                     /locus_tag="VFV99_13315"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5060339.1"
                     /translation="MTLDRMRAAATRPIDIASLAAFRIIFGAVMFAGIVRFVASGQLP
                     RIYGEPKFFFTYPGFDWVTPWPVTWMYVHYCVLAVLALAIAAGAWHRIACALFAVGFA
                     YTQLIDITNYLNHHYLVVLIAVQLALLPAHRTWSIDAWRKPALQAATIPAWIVWLLRF
                     QTGVVYVFAGLAKAKSDWLLHGQPLNTWLAARTETPLIGRFFDEPSVALAMSWAGFLF
                     DTTIVFWLSWARTRIPAYVALIAFHGLTGYLFMIGMFPLIMTSAALIFFPPDWPRTLL
                     HRIRLARFAVPRGGAVAPLSPRVSRVVAAAIAIHVIVQVALPLRHVVYPGAVLWNEDG
                     MRFAWHVMIREKHGAVTFVAELDGGKQLEIPPSTYLTWRQEREMAGQPDLILQLAHRI
                     GSDLVAQGHRDVRVRAVTSVSLNGRAPAPLIDPAVDLLGVRDIGERKWVLAAPDTDPP
                     VVQPLRQR"
BASE COUNT          828 a         1588 c         1622 g          775 t
ORIGIN      
        1 tgccccaacc ggcaaactcc gagtcgaacg cgtggcgaaa ccgcgaacgc gtccacgtgt
       61 acgcggcgct gatcggcagc acgagctcgc gcacgagcgg cacttcgccg ctcaattgcg
      121 cttcggcgcc ccacgtgaag acggcaccac cgttgtactc ggcaccttcc tgcgtctcgg
      181 tgcagccagc ggagagcgtg cacgatccct tgaggttcga gtagtcgctg aagaagccga
      241 tcacgtcggc ggcgatccgc tcgtcgcgcc agcgcgcgcc agcttcatag ttgatgctcg
      301 actcgggcag cacacccgag cccgccgacg gtgcgaccgg cacgaagccg cggtggacac
      361 cggcgaggac gcccgcgttg ccagtgacgt gatactcggc gccggcgccg ggaatcacga
      421 ccgcgtagct gccgtcgtgc tgcgccatcg tcagccaatc ggcgaaccgg tagtcgatca
      481 gctcgatgcg cgtgccggcc gcgagctcga agcgcttcca gtgcagcttg tcctgcgcga
      541 acagcgcgag cgcgatcgtc tcggcacgcg tgtcgagcac cgtcgcgcgc gaccgtgcgg
      601 tgcgcacgag cgtcgccatc gtcatgtcat aggtgtcttc gtagcgacgg cgatcagcgc
      661 gatcgaaatg gatgcgcgcg ccggcgtcga catgatgctt gaccggtccg gtcgcgcgtt
      721 cgagcgaaac cgtggtctgc gcgccttgcg acgtgaacgt gcgatcgttc gtgccgagca
      781 cgagcggctc ctccgggctc tgcgaatcgg cggcgccggt gaggatcgcg tagtagacct
      841 ggttcgcgcc ggcggtcggc tccgcgagca gcgcgtacag atcacgctgg ccgatcaggc
      901 cgtcgacctt gccccacacg cgatggaact tgtggcggta cgcgacagtc tcgacacgtg
      961 tcgcgactcc gaggtctacg cgatgggtcg cgcgaaaccg ccagtgcccc cagttcatct
     1021 cgtcgagctg cgaagcgacg tagcgccgtt gcggcgccgc ggcgaaatcc tcgtcggtga
     1081 ggccggtgta tgtctcgtgc gacgtctcgt cgccgtagcc gacgcgcaga tcgagctggt
     1141 ggtaggtctc tgcggtcggt tcggacatga accgcgtcgt gagctggacg tcgttcttgt
     1201 cgaagccggt cgggccaccg ccatcgaggt ccttgaagcc atcgctgcgg agatggacgt
     1261 actccgccat cacgccccag cgctcgcgcc gctcggcggc acgcgcgtgc agcttgccgt
     1321 agagatcgct gccaccggcg acgtcgacat aaccggagcg ctcggcgggg aacggctcgc
     1381 cgatcagatc gagcgcaccg ccgacggttg ccggcccgaa tcggatcgcc gacggaccct
     1441 tcgtcacctc gatgcgagac attcgcgtga cgagcggtac gtaataggca gcgggcgcgg
     1501 tgtagggagc cggcgcgatc aacacgccgt cttccatcag tgtgatcttc gcgctgcgat
     1561 cggcagcggc accgcgcatg ccgatgtttg ggcgcaggcc atagccgtct tcgtcgcgga
     1621 ggtagacgcc ggcgatgccg cgcaggacct tgtgcaaatc gtcgtgctcg tcgcgttcga
     1681 gcgcttcctt ggtgagctcg gcgcgcgcac cgggcggcgc cttgtcgacg acctcgatcg
     1741 tttcctcgcc ctcggcatcg gttgccgttg caggctgggg ggacgcaccg tcgacgacgt
     1801 cctgcgccag tgccggaacg acaaccagcg cgctggcggc gaccgtacta atcgttatcc
     1861 gaggcaacat catcgggaat gtccaaggcg agcagcgtga ggaactgact cttcagatcg
     1921 ttcgtgaact ctttggtcgc ggcgtgagct tcgacaatcg cggcgtagtc gttcgtcagc
     1981 gcgccgagga agctctccgg cagcgcgtcg gccttggcga tcgcggcgtc gagcgccgcc
     2041 gtcacccgcg tcgcgacctc gggctgaccg atcgcgatca agaagtcgtc gaagccgggc
     2101 ccgtcggcgg ccggcgtcgt gccggtgatc gcttcgcgca gcgtcgcaag gttgatccgg
     2161 atcgccgtcg tcgcgcgatc ggcgaagcgc agctcgacct cgcggatgca cggctcctgc
     2221 accgtgccgc acgcgttgac cgcaatgcct gcggcctcgg cgagcttcat gtccttgacc
     2281 atgcggtcga cgtagaaaaa cgaatcggac acgaggttca cgcccgcatg cgccgacggg
     2341 atcgatgtac ccattccggc gcgcgcgagc tcgccgacat agttgccgcc gtcagcgcgc
     2401 cacgcggtgt ggagcgtggt cgcggcagcg gccacgtcgg tcgcgagcac ctcggcgagc
     2461 cgacagcgcg cgcggagcag gtcagcgccg agggcgtccc agccgggcgg cgtgatcgca
     2521 cagttgtgcg cgtcggtctg cgggaacagc aggtattcga tcgcgagcag cgagcgctgg
     2581 cgctgcagct tggtcgagac gtcgtagctc gcaggattcg cccaccgcga cgcgacatcg
     2641 cgatcgacat cacacggcgc gagcagcggc cacgcgtaga tccgatcgcg cagcgctttg
     2701 ttgtccatcg cggccggacc aaccaacagc gcttcggcgt gttgccaggc atcgatcgcc
     2761 tcgcgccacg ccgcacgcgc ggactcgagt gtcgtaccgg gcgtgccggc atcgagcgca
     2821 tcgcagtacg cggcgatcgc ggccggcaat gcattcgcgc gagcgtcgaa cgtcgcctgg
     2881 atcggcaaca gcacgtgctc gccgagatgg gcgagtaacg cgttgcgatc gaagttgtcg
     2941 gggatcgcat cggccttcgc gggatcggaa ctgccacaac cggcgagcgc cgacgcggtc
     3001 gttgcgaggg ctgctagccc ggctgcgggg cctagagctg cggggcctag agctgcgagc
     3061 ccggcgagcg cgagcccggc tcgtcggccg gccaagatct gtccgcgcaa aatgcctgac
     3121 atcgttgaaa ggcatcctgg aggatttgaa aacgattttc aactccgatt tcagaaatct
     3181 gctgtgtcca atcgttcttg ctgtaataat tgacctttcc tgtcagcgtt ggcgcaatgg
     3241 ttggacgaca ggtgggtcag tatccggcgc cgccagcacc cacttccgct ccccgatatc
     3301 gcgtaccccg agcagatcaa ccgcgggatc gatgagcggt gcaggggccc ggccattcag
     3361 cgagacgctg gtgacggcgc gaacgcggac gtcgcggtgg ccctgcgcga cgaggtcgct
     3421 gccgatccga tgtgcgagct gcaagatcag atcgggctgt ccggccatct cgcgttcctg
     3481 tcgccacgtc agatacgtgc tcggcggaat ctcgagctgc ttgccgccat cgagctcggc
     3541 gacgaacgtc accgcgccat gcttctcgcg gatcatcacg tgccacgcga agcgcatccc
     3601 gtcttcgttc cacagcacgg cgcccggata gacaacatgc cggagcggca acgcgacctg
     3661 cacgatgacg tggatcgcga tcgcagccgc cacgacacga ctcacgcgcg gcgagagcgg
     3721 cgccactgcg ccaccacgcg gaaccgcgaa tcgcgcgagc cgaattcgat gtagcagcgt
     3781 gcgcggccag tccggcggaa agaagatcag cgcggcgctc gtcatgatga gcgggaacat
     3841 gccgatcatg aacaagtagc cggtgagccc gtggaacgcg atcaacgcga cgtacgccgg
     3901 aatgcgcgtg cgcgcccacg acagccagaa cacgatcgtg gtgtcgaaca agaagccggc
     3961 ccacgacatc gcgagcgcga cgctcggctc gtcgaagaag cggccgatca gcggcgtttc
     4021 ggtgcgcgca gcgagccacg tgttgagcgg ctgcccgtgc aatagccagt cgctcttggc
     4081 cttcgcgagc ccggcgaaca cgtagacgac accggtctgg aagcgcagca gccacacgat
     4141 ccacgctggg atcgtcgccg cttgtagcgc tggcttgcgc cacgcgtcga tcgaccatgt
     4201 gcgatgcgcc ggcaacagtg cgagctgcac cgcgatgagc acgacgagat agtggtgatt
     4261 gagatagttc gtgatgtcga tcagctgcgt gtacgcgaag ccaacggcaa acaacgcgca
     4321 cgcgatccga tgccacgcgc cggcggcgat cgcgagcgcg agtaccgcga gcacgcagta
     4381 gtgcacgtac atccaggtca ccggccacgg cgtgacccag tcgaatcccg gatacgtgaa
     4441 gaagaacttc ggctcgccgt agatccgcgg cagctgcccc gatgcgacga agcgcacgat
     4501 gccggcgaac atcacggcgc cgaagatgat gcgaaacgcc gcgagcgatg cgatgtcgat
     4561 cggtcgcgtg gcggcggcgc gcatgcggtc gagcgtcacg cgctcaccct actcgaagtc
     4621 gcacgcgtta ccagcgcgct atttgcgttc gaggatgatc aggtcgacgc gctcgttgat
     4681 tcccttcgca ccgcgcgtgg tcgggtcgac gagcggcttc gtgccgccaa agccgcgtgg
     4741 ctcgaggcgc ttctcctcga tgccgtactt gatcagccac tcgcgaatcg cgaacgcgcg
     4801 cagctcggag acg
//
LOCUS       DASUFX010000333         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_606127,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000333 DASUFX010000000
VERSION     DASUFX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606127"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFV99_13320"
     CDS             <1..702
                     /locus_tag="VFV99_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5060340.1"
                     /translation="MIRITSMDALVVNGIGHDTWAFEILDASGMRDKLKLIYANDGVS
                     LIPVAGDPSGEKVVNPHTFISTTAAIQQVYTISQKLGELDPENAMYFRQNARKYALEI
                     RKLRSEFDAQIKGVSLSSFRCATMHSGYDYILQELGLQVTAVIEPRHGVEPTARQLAE
                     TIDRIKAANVNVLFAEKYFASKLSDTIRDATGVKMYSISHISSGEYTPTKFVEEMREN
                     LTTLATAIKESGASG"
     gene            707..1432
                     /locus_tag="VFV99_13325"
     CDS             707..1432
                     /locus_tag="VFV99_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX5060341.1"
                     /translation="MNGPTIEFDRVNLTLGATSILEDVTFTVRAATIHCIVGANGGGK
                     TSLVRSLLGQMPHKGKISIHWADSRVIGYVPQTLDFDKSLPITVGDFMAMATQRRPVF
                     LGVSKARRDEIDRMLERLGLNGKRKAKLGSLSGGERQRVLLAQALIPEPALLVLDEPT
                     TGLDLKGKEILQRTIVEFARSGGTVVWINHDIAQVGEIADALTYIDRKVLLDGAPREA
                     LATGTAAHLFPTLASTFAAAVQS"
     gene            1429..2334
                     /locus_tag="VFV99_13330"
     CDS             1429..2334
                     /locus_tag="VFV99_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5060342.1"
                     /translation="MTWLFDIPRQFLIGLADDGVIHYTFSYAFVVNGFLCAFVIGPLL
                     GGIGTMVVAKRMAFFSQAVGNAALTGVAIGVIIGESYTAPYMSMFSFCLLFGLLLNFS
                     RNRTKMSADTLIGVFLSISLAIGATALLWVSAKVNTHILDTVMFGSILTVDDTDMTVL
                     LITTVLTLVTALPLFNQMLLGSLNPSLAHVRGVRVHLLEYVFVLLLTILTVACVKIVG
                     AVLVEALLLIPAAAARNLNRSIRGFVWWSIAFSTIACLAGIYAPMRWDLPLPSGGAII
                     LAAAMMFVVTLVVRMALPRYREAAL"
     gene            2331..>2798
                     /locus_tag="VFV99_13335"
     CDS             2331..>2798
                     /locus_tag="VFV99_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060343.1"
                     /translation="MKLNRIGVLAGVLAAVSLQQSVGAADAAPPLVLTGTGATYSLTV
                     ALTAGTPIQVQNVPADGRQLALLKDYIERRKESLEPTFAAATAVVTLTNALPGDPLYR
                     FARDANIRIVDIEAGLPWSLDAPGVALADAPSSKAEWGGDNAETAVAPYFWLSL"
BASE COUNT          464 a          923 c          907 g          504 t
ORIGIN      
        1 atgatccgca tcacgtcgat ggacgcgctc gtcgtgaacg gcatcggcca cgacacgtgg
       61 gcgttcgaga tcctcgacgc ctcgggcatg cgcgacaagc tcaagctcat ctacgcgaac
      121 gacggcgtgt cgctgatccc ggtggctggc gacccgagcg gcgagaaggt cgtcaacccg
      181 cacaccttca tctcgacgac ggcggcgatt cagcaggtct acacgatctc gcagaagctc
      241 ggcgagctcg accccgagaa cgccatgtat ttccggcaga acgcgcggaa gtacgcgctc
      301 gagattcgca agctgcgctc cgagttcgac gcgcagatca agggcgtgag cttgtcgagc
      361 tttcgctgcg cgacgatgca cagcggctac gactacatcc tgcaagagct cgggctgcag
      421 gtcacggccg tcatcgagcc gcgccacggc gtcgagccga cggcgcgcca gctcgccgag
      481 acgatcgatc gcatcaaggc cgccaacgtc aacgtgttgt tcgccgagaa gtacttcgcg
      541 agcaagctgt ccgacacgat ccgcgacgcg acgggcgtga agatgtactc gatttcccat
      601 atcagcagcg gcgagtacac gccgaccaag ttcgtcgaag agatgcgcga gaacctgacg
      661 acgctcgcga ccgccatcaa ggagtccggc gccagcggct aagcgaatga acggacccac
      721 gatagagttc gaccgcgtca atctgacgct cggtgccacg tcgatcctcg aggacgtgac
      781 gttcacggtg cgcgccgcca cgatccactg catcgtcggt gccaacggcg gcggcaagac
      841 gtcgctcgtg cgctcgctgc tcgggcagat gccgcacaaa ggcaagatca gcatccattg
      901 ggccgacagc cgcgtcatcg gctacgtgcc gcagacgctc gacttcgaca agagcctacc
      961 gatcacggtc ggcgatttca tggcgatggc gacgcagcgc cggcccgtgt tcctcggcgt
     1021 ctccaaagcg cggcgcgacg agatcgaccg catgctcgag cggctcggct tgaacggcaa
     1081 gcgcaaggcg aagctcggca gcctgtcggg cggcgagcgc cagcgcgtgc tgctggcgca
     1141 agcgctgatt cccgagcccg cgttgctcgt gctcgatgag ccgacgaccg gcctcgactt
     1201 gaagggcaaa gagattctgc aacgcacgat cgtcgagttc gcgcgcagcg gcggcacggt
     1261 ggtgtggatc aaccacgata tcgcgcaggt cggcgagatc gccgacgcgc tgacgtacat
     1321 cgaccgcaag gtgttgctcg acggcgcgcc gcgcgaggcg ctcgcgacgg gcaccgccgc
     1381 gcacttgttc ccgaccttgg cctccacctt cgccgcggcg gtgcagtcat gacttggttg
     1441 ttcgacatcc cgcggcagtt cctcatcggc cttgccgacg acggcgtcat ccactacacg
     1501 ttctcatacg cgttcgtcgt caacggcttc ttgtgcgcgt tcgtgattgg gccgctgctc
     1561 ggcggtatcg gcacgatggt cgtcgcgaag cgcatggcgt tcttctcgca agccgtcggt
     1621 aacgcggcgc tcacgggcgt ggcgatcggc gtcatcatcg gcgagtcgta tacggcgccg
     1681 tacatgtcga tgttcagctt ctgcctgctg ttcgggctgc tgctgaactt ctcgcgcaat
     1741 cgcaccaaga tgtcggcgga cacgctgatc ggcgtgttcc tctcgatctc gctggcgatc
     1801 ggcgcgacgg cgctcttatg ggtatccgcc aaggtcaaca cgcatatcct cgacaccgtg
     1861 atgttcggct cgatcctgac cgtcgacgac accgacatga ccgtgctctt gatcacgacg
     1921 gtgctaacgc tcgtcacggc gctgccgctg ttcaatcaga tgctgctcgg cagcctgaac
     1981 ccgagcctcg cgcacgtgcg cggcgtgcgc gtgcacctgc tcgagtacgt gttcgtgctt
     2041 ctcttgacga tcctgaccgt cgcttgcgtg aagatcgtgg gcgccgtgct cgtcgaggcg
     2101 ttgctgctga tcccggccgc ggccgcacgc aacctgaacc gctcgatccg cggcttcgtc
     2161 tggtggagca tcgcgttctc gacgatcgcg tgcctcgccg gtatctacgc gccgatgcgc
     2221 tgggacctgc cgttgccgtc cggtggcgcg atcatcctcg cggccgccat gatgttcgta
     2281 gtcacgctcg tcgtgcggat ggcgttaccg cggtatcgcg aggctgccct atgaagctaa
     2341 atcgcatcgg tgttttggcc ggcgtgctgg ccgcggtttc gttgcagcag tcggtgggcg
     2401 ctgccgatgc ggcgccgccg ctcgtgttga ctggcactgg ggcgacgtac tcattgacgg
     2461 tcgcgttgac ggccggcacg ccgattcaag tgcagaacgt gccggccgat gggcggcagc
     2521 tcgcgctgct caaggactac atcgagcggc gtaaagagtc gttggagccg acgttcgccg
     2581 cggcgacggc ggtcgtgaca ctgacgaacg cgctgcccgg cgacccgcta taccgcttcg
     2641 cgcgcgacgc caacatccgc atcgtcgaca ttgaggcggg cctgccgtgg tcgctcgacg
     2701 cgccaggcgt cgcgctcgcg gacgcgccct cttcgaaggc cgagtggggc ggcgacaatg
     2761 ccgaaacggc cgtcgcgccg tacttctggc tgagcctc
//
LOCUS       DASUFX010000334        13135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1301438, whole genome shotgun sequence.
ACCESSION   DASUFX010000334 DASUFX010000000
VERSION     DASUFX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301438"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VFV99_13340"
     CDS             <1..1477
                     /locus_tag="VFV99_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5060344.1"
                     /translation="LRYELSSAFTKVLRQERSVNVRGLKVGTNGGTQYVSLTVQKLTE
                     PRELQGTVIVVISDVPGVPEKAPPARGRRKLPPRLAELEDELQRSRDETQTVREEMQT
                     SQEELKSTNEELQSTNEELQSTNEELTTSKEEMQSLNEELQTVNHELQSKVDELSRSN
                     NDMKNLLNSTDIATLFLDTDLNVRRFTTATAKIIKLIPADVGRPITDIASDLDYANLA
                     EDAREVLRTLIYKENQTSTRDGRAFSVRIMPYRTLENVIDGVVITFTDASAARALERA
                     ATDQASQLRQMAEALPHLVWGARPDGAFDFLSRQWGEYTGLNDGEQLLWGWLDQLHPS
                     DRDRVREQWRTAVKTGSHFDSEFRIRSNTGEYRWFKARATPIRDQSGAIVKWYGTSTD
                     VDELKRAEERLGALTQVVSDAFVGVDVDLQIVEVNPVFEKLISRSAAELLGRTLTDIL
                     PESPLDLTHAPKDLQVNIGGRTYNARSRRDPAGGYWLFVRA"
     gene            1487..1849
                     /locus_tag="VFV99_13345"
     CDS             1487..1849
                     /locus_tag="VFV99_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5060345.1"
                     /translation="MPPRKTTTLRRRAERTLQQRKTRKPSPRSRLIHELEVHEVELEL
                     QNEELVEARERVEAALERYTEVFDFAPIGYVTLDARGAVREINHAGAALLGAQRTALI
                     EQQFASNVTPKSLPTFRR"
     gene            1879..3180
                     /locus_tag="VFV99_13350"
     CDS             1879..3180
                     /locus_tag="VFV99_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5060346.1"
                     /translation="MSCELDLRRDQIAFPARLTAAVLRRGEPVVMIAFEDVSERRANE
                     AELARSEAALREMNERKDEFLAMLSHELRNPLSPIRTSVAVLQLAEPGSRIATHSIEI
                     IDRSAAHMNRLVDDLLDITRITHGKIELRREIVDLTALVRRVIDDQRDVFSANHIEIA
                     TTLPSHELLLDADAARLVQVMMNLISNALKFTPKHGRIAIELAREGNAGVISVVDNGI
                     GIAPELIGELLRPFAQVPQALDRNHGGLGLGLAMVKSLVELHGGRVTIRSAGLGHGTE
                     VKVVLPIQQASSAVRAVDLAPVSARRRVLIVEDMPDNARSMEQALKLRGHDVEVALNG
                     FTALERLATFKPEVVLCDIGLPDMDGFAFAMRVRADEALRHVCLVALSGYARAEDVRR
                     AKAAGFDRHLAKPARLDQIDRLLAELVPRTTRDPFDNNTLH"
     gene            complement(3183..4142)
                     /locus_tag="VFV99_13355"
     CDS             complement(3183..4142)
                     /locus_tag="VFV99_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5060347.1"
                     /translation="MNRDIITIGASAGGVEVLLEMAHALPKDLPAAVFVVVHVPVGFV
                     SSLPELIARRSALEARHPLHDEAIEHGTIYVAPPDMHLQVRPGMIEVVRGPKDNGFRP
                     AADVLFRSASNAYGSRVIGVVLSGYRDCGTAGMLSVKARGGLAVVQDPTSAVAPEMPQ
                     NVIDSVAVDHVVHPLELPGLLARLVTTEAGPAREPGQLVKQVEGTEKGLPSDIVCPIC
                     EGVLTETQPGLFQHFRCHVGHGFSLESLVREQGEEMERVLWAAIRALEESAALSHRIS
                     LRETGELRKRFEEKAATQTQQANYIRELVLHGARLDRADASKL"
     gene            complement(4235..5869)
                     /locus_tag="VFV99_13360"
     CDS             complement(4235..5869)
                     /locus_tag="VFV99_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060348.1"
                     /translation="MSNNDKPGSTGEQPTPEELLGIGAGEDILIVDDNEANLIAFDSA
                     LAPLGRRLVMVQSGVQALAEMLAQDFALVILDVSMPGMTGIECAEMIRKRKRTRGIPI
                     IFVTGLTVQDTAILEGYDAGGFDFVVKPVRAEVLRAKVRVFLQLQERTRAMQHHAAAR
                     HELEIAANKAKLREQEQRHESEMLEMRVQQLAEMDRRKDELLAILGHQLRNPLQALQL
                     AIDALHGQDVPAELVDRIHNIIERRVRSLNVVVDDLLDIAKLSTGELELRAHPLKLAD
                     LIRQVVETVDPMLDGRAIDLALDDEVVVAGDAARLVRCIANVVDNAIKFTERDGRITI
                     KLTRSAGRALLRVTDNGRGITAALLPQLFDVFSRTTRGTSGVGLGLAIVRRLVGVHGG
                     RVRAFSEGEGRGATFEISLPLAATAAPRVEEDLRPTRSLRVVVCEDAHDIRELAADLL
                     RADGHTVYQAADGATAIKLIETERPDVALVDLVLPDIDGFTVARTVRRTLGTSAPRMV
                     AITALASATPQADFDEHLLKPASRERMLSVLARLSR"
     gene            complement(6038..9121)
                     /locus_tag="VFV99_13365"
     CDS             complement(6038..9121)
                     /locus_tag="VFV99_13365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5060349.1"
                     /translation="MLERFITASFRKPLIAIVLALAGAAMGTVWMRELPRDVFPDLSA
                     PVFNIIVQNSAMNADELEMRVAIPFETALSGLPQVRRVRSSSTLGVVQVTIEFEPDAD
                     YSRSRQYVAERLPQVDLPPGTETPLLSGVTGRLNEIFEITVEARSGIADLMTLRDLAE
                     NDIKNRLLAVPGVAAVERLGGYLREFQIQMDPDRMAARGVTLDDIMHAARGANVSAAG
                     GFVTQAQMEWTVRAAGRAANVEQLRSTVVAMKGGTPVLLGDVGEVREAPAVRRGIAHR
                     LNGEVVSCRIVKQFGADTVSVARGIRAAIADLKTSLPRGVEIRTVYDQSQLVESSLGG
                     VGRAVLIGACLVALVLFVLLGNVRAALIVTLTLPLSLALSGVFMRVAGIGINTMTLGG
                     LAIAVGLLVDSSIIVVENIVHRISQRREGDRHGNALAAAIEVARPIAFATVVVIAVFI
                     PLFAMTGIEGRMYKPLAGAVIAAVLAALVLALALVPVVAAGLLRPTREGAHEDVWLIR
                     KLKGAYRPLLDRALRHTALVMIITLSITAPALWVATKLGSDFVPPLDEGAILMQTFLP
                     PEASLDEVDRLNHRVEDVLRGFPEVSDVVRRTGRAERTEDPMPHTLSDVLILLKPDRA
                     RGGEALVSDMREAVEKVPGVSALFTTPLGMRIDEGLGGTPADISVRIFGPDAQKLADL
                     ADAAAAMMRGVEGIEDVRAEPLVGLPQLTVQVDREASARVGLTPGDVIEAVQIGLAGE
                     HVSEVWIGQRRFDLVVKLSPDFRRDAATLAALMVDGHDGSRIPLGKLAQIEPTFGLGT
                     IKREAGSRRIAVEASVSGRDLGSAARDVKERLAAKLKLPPGYFVDVGGKVESQTRAQQ
                     ALYLAVGLALLAVFLLLYIALGSLAETIVILATLPSAFVGGIVALLIAGESWSVSSLV
                     GLIGLFGIAVQNGLVLVTQTRGSISEGHGFTQALREACIGRVRPKIMTASTAILGLLP
                     LLVLDLHGTEIERPLAIVMIGGLLTSTVFTLLALPTFYQLVHNLGVRWRSVPAAT"
     gene            complement(9131..10318)
                     /locus_tag="VFV99_13370"
     CDS             complement(9131..10318)
                     /locus_tag="VFV99_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5060350.1"
                     /translation="MNKLTMLALLLIGGCGGKKTEPNQDDYTSPEPAGHAGSDTAEHG
                     NAHEHGPEMIKIAPDMMRDLRVTTGKAQARAAAETVSALGELHVNEEAYAEIAAPVSA
                     RVTKILAKPGDTVKAGQVLAELASPELAQGRAEVGAAHSRVDVARANAERKRKLAADR
                     LIPERELIEAEAALTEAEAAYKVTASSLQKFGGASGDSALALKSPIAGTVIDRSVVMG
                     QLADPSKTLFRVGDLSTLWLHAHVFERDAVRVQPGRTATATFAALPGKSTEATIKWIG
                     REVDQASRTIDVRLDVPNSDGLLRPGMSATVALPLGEVGPDTVVTVPAAAVQRVGATW
                     AVFIPHGPGEFAVRSIGRGRDLSGEVEVLSGLQPGDEVVVEGAFLLKAEADKASGAGG
                     HED"
     gene            complement(10315..11535)
                     /locus_tag="VFV99_13375"
     CDS             complement(10315..11535)
                     /locus_tag="VFV99_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5060351.1"
                     /translation="MSQVPFARLALAALVLHSLPASADQIDLDLPNAIARAHQAAPAA
                     VGARGQIAIANAAVTGANLPFIDNPEIEAGAGPRLISTRPTDVDARIEQNLELGRRSP
                     RRQLARAGVTQTQAELDAMLRELDLEVTKAFYDALFAVRAAELAKRAEDFAQRAAAVA
                     DRRRKAGEITDLDANLARTALGRARSATQSAQSDQASAVGRLAALIGATPDDAIVVRG
                     DLKPAALPDLATLRGTAGSRADIRVLDAERGVARAERAQASANGLPQVWLWAGYQRED
                     TQSIVLGGLRMTFPLWNRAQGEKAAATARERRASDMRATTLRAAGRESVDAFAAYTSA
                     RLAVEGFETDVVPLLDDSEQLLQKTIDAGQIAVSDYLVARQQILDGRREHLERLLALA
                     KAAATARFVAGGAP"
     gene            complement(11607..11900)
                     /locus_tag="VFV99_13380"
     CDS             complement(11607..11900)
                     /locus_tag="VFV99_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060352.1"
                     /translation="MLVLALLQATGIAELVRRHACEIECRDDGCGNDCTSGNDAPSCP
                     CHCPSVQSQTPPAIASVTMPPPTEAAPVVFDNADRMHGSPDPADILHIPKHVV"
     gene            12183..12863
                     /locus_tag="VFV99_13385"
     CDS             12183..12863
                     /locus_tag="VFV99_13385"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HEX5060353.1"
                     /translation="MGTKLTPQVLEAIRVDREEHGFTLTRIMERHGLRRTTAYTAIAG
                     LDDSKVQRASPTRRVVQPVDAPPRPPLSKANLGEAARQLIAGRMLLAGLDVFQPIGED
                     TPIDLLVLRPDGVALKCQCKVMYIAKTGVHVMPFCTIRKWGPNAKVVPHRYTRDEVDF
                     FLGYAVEVDAVFVFPFDATAHFKSSMTVWLLREPANNNGTSRFDAAPYRNAFHLLSAQ
                     DGHPTLDK"
     gene            complement(12867..12941)
                     /locus_tag="VFV99_13390"
     tRNA            complement(12867..12941)
                     /locus_tag="VFV99_13390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12904..12906),aa:Arg,seq:cct)
BASE COUNT         2351 a         4273 c         4277 g         2234 t
ORIGIN      
        1 actgcgctac gagctctcga gtgcgtttac gaaggtgctg cgtcaggagc gcagcgtcaa
       61 tgttcgcggc ctcaaagtcg gaacgaatgg tgggacgcag tacgtgagcc ttacggtcca
      121 gaagctcacc gagccgcgcg agctgcaggg taccgtgatc gtcgtgatct ccgacgtgcc
      181 cggcgtgccg gagaaagcgc cacccgcaag gggacgtcgc aagctgccgc cccggcttgc
      241 cgagctcgag gacgagctcc agcgctcgcg agatgagacc cagacggtcc gcgaagagat
      301 gcagacctcg caagaagagc tcaagtcgac gaacgaggag ctgcaaagca ccaacgaaga
      361 gctgcagagc acgaacgagg agctcacgac ctcgaaagag gagatgcagt cgctcaacga
      421 ggagctgcaa acggtcaacc acgagctgca atcgaaggtc gacgagctct cgcgctcgaa
      481 caacgacatg aagaacctct tgaacagcac cgacatcgcg acgctgttcc tcgataccga
      541 cctcaacgtg cgccgcttca cgaccgcaac tgcaaagatc atcaagctca ttcccgcgga
      601 cgtcggcagg ccgatcaccg acatcgcgtc ggatttggac tacgccaacc tcgccgaaga
      661 cgcgcgcgag gttcttcgca cgctgatcta taaggagaac caaacatcga cgcgtgatgg
      721 acgagcgttc tcggtccgca tcatgccgta tcgcacgctc gagaacgtga tcgatggcgt
      781 cgtgatcacg ttcaccgacg ccagtgcggc acgggcgctc gagagagcag cgaccgacca
      841 agcgtctcag cttcgtcaga tggccgaagc gctgcctcac ctcgtgtggg gcgcgcggcc
      901 ggatggcgcc ttcgacttct tgagccggca atggggtgaa tacaccggcc tcaacgatgg
      961 tgagcagctg ctgtggggct ggctcgatca gctacatccg agcgaccgcg atcgtgtccg
     1021 cgaacagtgg cggactgcgg tcaaaaccgg ctctcacttc gacagcgagt tccgcattcg
     1081 gagcaacacc ggggaatatc gctggttcaa ggcgcgcgcg acgccgatcc gcgatcagag
     1141 cggtgcgatc gtgaagtggt acggcaccag cacggatgtc gacgagctca agcgggcaga
     1201 agagcgcctg ggagcgctca cgcaggttgt gagcgatgca ttcgttggcg tcgatgtcga
     1261 tcttcagatc gtcgaggtga acccggtgtt cgagaaattg atctcacgca gcgccgcgga
     1321 gctcctcggg aggacgttga ccgacattct tcccgagtca ccactcgacc ttacccacgc
     1381 acccaaggac ctgcaggtga acatcggagg gcggacatac aacgcacgca gccggcgtga
     1441 tccggcgggt ggctactggc tgttcgtgcg agcatgatct ccgacgatgc cgccgcgcaa
     1501 gacgacaacc ctgcggcgcc gcgccgagcg gacgctgcag cagcgcaaaa cgcgtaagcc
     1561 atcgccacgt tcacgcctca tccacgagct cgaggtccac gaggtcgagc tcgagctgca
     1621 gaatgaagag ctcgtcgaag cacgcgaacg tgtcgaagct gcgctcgagc gatacaccga
     1681 ggtctttgac ttcgcgccga ttgggtacgt gacactcgac gcgcgtgggg cggttcgcga
     1741 gatcaaccac gcgggtgcgg cgttgcttgg cgcgcaacgc acggcgctca tcgagcagca
     1801 gttcgcgtcg aatgtgaccc caaaatccct gccgaccttc cggcgctgat cgagcaagca
     1861 catggcacga cgacgtccgt gagctgcgag ctcgacctgc gtcgcgatca gattgcgttt
     1921 ccagctcgac tgactgccgc ggtcctgcgg cgcggtgaac ccgtggtgat gatcgcattc
     1981 gaggacgtgt ccgagcgccg agccaacgaa gccgagcttg cccgcagcga ggccgcattg
     2041 cgcgagatga acgaacgtaa agatgagttc ctcgcgatgc tatcgcacga gctccgcaat
     2101 ccgctctcgc cgatccgcac cagcgttgct gtgttgcagc ttgcggagcc gggctcgcgc
     2161 attgcgaccc actcgatcga gatcatcgat cgctcggctg cacatatgaa ccgactggtc
     2221 gacgacctgc tggacatcac gcggatcacc cacgggaaga tcgagctacg gcgtgagatc
     2281 gtcgatctga ctgccctcgt tcgccgcgtg atcgatgacc agcgcgacgt gttctcggca
     2341 aaccacatcg agatcgcgac gacgttgccg tcacacgagc ttctgctcga tgccgatgca
     2401 gcccggctcg ttcaggtgat gatgaacctg atctcgaacg ccctgaagtt cacgccgaaa
     2461 cacggccgga tcgcgatcga gctcgcgcgc gagggtaacg ccggtgtgat ttcagtcgtc
     2521 gacaacggta tcggcatcgc gcccgagctc attggtgagc tgcttcgccc gttcgcacaa
     2581 gtaccacagg cgctcgatcg caatcacggt ggcctcgggc tcggactcgc gatggtcaag
     2641 tcactcgtcg agctccatgg cggacgggtc acgatccgca gcgcaggcct cggtcatggc
     2701 accgaggtca aggtcgtatt gccgatccaa caagcgtcat ccgccgtccg agccgtcgac
     2761 ctcgcgcccg tcagcgcgcg gcggcgcgtg ctcatcgtcg aggatatgcc ggacaacgcg
     2821 cgcagcatgg aacaagcgct gaagctgcgg ggccatgacg tcgaggtggc gctcaacgga
     2881 ttcaccgcgc tcgaacgact cgcaacgttc aagcccgagg tcgtgctgtg cgacatcggg
     2941 cttcccgaca tggacgggtt tgcgttcgcg atgcgggtcc gtgcagacga ggcgctccgt
     3001 cacgtctgcc tcgtcgcgct cagtggctat gcgcgcgcag aagacgtcag gcgtgcgaag
     3061 gccgccgggt tcgatcgtca cctcgcgaaa cccgcgcggc tcgaccagat cgatcgcctg
     3121 ctcgcggagc tcgtgccgcg gaccacacga gatccattcg acaacaacac gctgcactag
     3181 ccctagagct tgctcgcgtc tgcgcgatcg agtcgggcac cgtgaaggac gagctcgcgg
     3241 atgtagtttg cttgctgcgt ctgcgtagcg gccttctctt cgaagcgttt gcgtagctcg
     3301 cctgtctctc tgaggctgat ccggtgcgac agtgcagcgc tttcttcgag cgcacgaatc
     3361 gcagcccaga gcacgcgctc catttcttcg ccttgttcgc ggacaagact ctcgagcgag
     3421 aagccgtgcc cgacgtgaca gcgaaagtgt tggaacaggc cgggctgggt ctccgtcagc
     3481 acgccttcgc agatcggaca cacgatgtca ctcggcaggc ccttctcagt gccttcgacc
     3541 tgcttcacga gctggcccgg ttcacgcgcg ggacctgctt ctgtggtcac gagtcgtgcg
     3601 agcagccccg gaagctctaa tggatggacg acgtgatcga cggcgacgct gtcgatcacg
     3661 ttctgcggca tttctggtgc aaccgcgctc gtcggatcct gcacgaccgc gagcccaccg
     3721 cgcgccttca cggacaacat gccggcggta ccgcaatccc gatagcccga cagcacgaca
     3781 ccgatcacgc gcgagccgta cgcgttcgac gcgctacgaa acagcacgtc ggcggcggga
     3841 cggaacccgt tgtccttcgg gccgcgcacg acttcgatca tgccgggccg cacttgcagg
     3901 tgcatgtccg gcggcgcgac atagatcgtg ccgtgctcga tcgcttcatc atgaagcgga
     3961 tggcgcgcct cgagcgcact gcgtcttgcg atgagctccg gcagggagct gacaaacccg
     4021 actggcacgt gcaccacgac aaacaccgcg gcaggcaaat ccttgggcag tgcatgcgcc
     4081 atctcgagca ggacttcgac tcctcccgcc gaagccccga tcgtaatgat gtcgcggttc
     4141 atgaggacag gctggatcgt tcgtcgccga tgccaaccgt gcgggcacgc gcactcgacg
     4201 cgtgcgcctt cgcccatgtg tcgacgtcgc gcggtcagcg ggacagacgc gcgagaaccg
     4261 agagcatgcg ttcgcggctc gccggcttga gcaagtgctc gtcgaagtcg gcctgtggtg
     4321 tggcgcttgc gagcgctgtg atcgcgacca tccgaggcgc cgatgtgccg agcgtacgtc
     4381 gcaccgtgcg ggcgaccgtg aagccatcga tatcggggag cacgaggtcg acgagcgcga
     4441 cgtcgggccg ctcggtctcg atcagcttga tcgcggtcgc gccgtcggcg gcctggtaca
     4501 cggtgtgacc atccgcgcgg agcagatccg cggcgagctc gcggatgtca tgtgcgtctt
     4561 cgcagacaac cacgcggagc gagcgagtag ggcgaaggtc ttcttcgacg cgcggtgcgg
     4621 cagtcgctgc cagcggcaga ctgatctcga acgtagcgcc acggccttcg ccttcgctga
     4681 acgcgcggac gcgaccaccg tgaacgccga cgaggcgacg cacgatcgca aggccgaggc
     4741 cgacgccgct tgtacccctg gtcgtacgcg aaaacacgtc gaagagttgc ggtagtagtg
     4801 ctgcggtgat gccgcggccg ttgtcggtca cccgcaggag tgcacgaccg gcgcttctgg
     4861 tcagcttgat cgtgatgcgg ccgtcacgct ccgtgaattt gatcgcgtta tcgacgacgt
     4921 tcgcgataca gcgcacgagc cgggcggcgt cgccggcgac gaccacctcg tcatcgagcg
     4981 cgaggtcgat cgcgcggcca tcgagcatcg ggtcgacggt ctcgaccacc tggcgaatga
     5041 ggtcggcgag cttgagcgga tgagcccgca gctcgagctc tcctgtggat agcttcgcga
     5101 tgtccagcaa atcatcgacg acgacgttga gcgagcgcac gcgcctctcg atgatgttgt
     5161 ggatgcgatc gaccagctcg gccgggacat cctggccatg caacgcatcg atcgcgagct
     5221 gcagcgcctg cagcggattg cgcagctgat ggccgaggat cgcgaggagc tcgtccttgc
     5281 gccgatccat ttcagcaagc tgttgcacgc gcatttcgag catctcggac tcgtgacgct
     5341 gctcttgctc gcgcagcttc gccttgttcg ccgcgatctc gagctcgtgg cgcgccgctg
     5401 cgtggtgttg catcgcgcgc gtgcgttctt gcagctggag aaacacgcgc accttcgcgc
     5461 gcaacacctc ggcgcgcact ggcttgacga cgaaatcgaa gccgccggcg tcgtagcctt
     5521 cgaggatcgc ggtgtcttgc accgtcaaac cggtgacgaa gatgatcggg ataccgcgcg
     5581 tgcgcttgcg cttgcggatc atctcggcgc actcgatgcc ggtcatgcct ggcatcgaca
     5641 cgtcgaggat cacgagggcg aagtcttgcg cgagcatctc cgcgagtgcc tggacgccgg
     5701 actgcaccat gacgagccgg cgcccgagcg gcgcgagcgc ggagtcgaac gcgatgaggt
     5761 tggcctcgtt gtcgtcgacg atcagaatgt cttcgccggc gccgatgccg agcagctctt
     5821 cgggcgtcgg ctgctcgcca gtggaccctg gcttgtcgtt gttactcata cttcgtgtcg
     5881 aggtccccca accccaagaa gtgcgaaagg acaagcaaag atcgtgcgga cgtaacccgg
     5941 cgtacgcagg ttcgcgagag tgttctggcg tagagaaagc gcacggtatg aaacccgtac
     6001 gtgcgcgagc gccacagccc gatgcggaat ccagggccta tgtcgcagcc ggtacggagc
     6061 gccagcgcac gccgaggttg tggaccagct gatagaacgt cggcagcgcc agcagtgtga
     6121 acacggtcga cgtgagcaaa ccgccgatca ttacgatggc gagtggccgc tcgatctcgg
     6181 tgccgtggag gtcgagcacg agcagcggca ggagcccgag aatcgcagtc gacgcggtca
     6241 tgatcttggg ccgcacgcgt ccgatgcagg cctcgcgcag cgcttgcgtg aagccgtgac
     6301 cctcgctgat cgacccgcgc gtctgggtca cgagcacgag accgttctgt accgcgattc
     6361 cgaacagacc gatcagaccg acgagtgatg acacgctcca gctctcgcca gcgatcagca
     6421 gtgcgacgat gccgccgacg aacgcgctcg gcagtgtcgc gaggatcacg atggtctcgg
     6481 cgagcgagcc gagtgcgatg tagagcaaga ggaacactgc gagcagcgcg agcccgacgg
     6541 ccaggtacaa cgcttgttgc gcgcgcgtct ggctctcgac cttgccgccg acatccacga
     6601 agtagccggg cggcagcttc agcttcgcgg cgaggcgttc cttcacgtcg cgcgctgcag
     6661 agccgagatc gcggccggac acgctcgcct cgacagcaat gcggcggctg cctgcctcgc
     6721 gcttgatcgt gccgagtccg aacgtcggtt cgatctgggc gagcttaccg agggggatgc
     6781 ggctgccatc atggccgtcg accatgagtg cggcgagcgt cgcggcgtcg cgccggaagt
     6841 ccggcgacag cttcacgacc agatcgaagc gtcgctgacc gatccacacc tcggagacgt
     6901 gctcgccggc gagcccgatt tggaccgctt cgatcacatc gccgggcgtg agtccaacgc
     6961 gtgcggaagc ttcgcgatcg acctgcacgg tcaactgagg caggccgacg agcggttcgg
     7021 ctcgcacgtc ctcgatgccc tcgacgccgc gcatcatcgc ggcggctgca tccgcgagat
     7081 cggcgagctt ctgcgcgtcg gggccgaaga tgcggaccga aatatcggcc ggtgtgccgc
     7141 cgaggccctc atcgatacgc atgccgagcg gcgtcgtgaa cagcgccgaa acgccgggta
     7201 ctttctcgac cgcttcgcgc atgtcgctga ccagcgcttc gccgccgcgt gcgcgatcgg
     7261 gcttgagcag gatcagcacg tccgacagcg tgtgcggcat tggatcctcg gtgcgctcgg
     7321 cgcgccctgt gcgccgtacg acgtcggaga cttcagggaa gccgcgcagc acgtcctcga
     7381 cgcgatggtt cagccggtcg acttcatcga gagacgcttc gggcgggagg aatgtctgca
     7441 tcaagatcgc gccttcgtcg agtggcggta cgaagtccga accgagcttc gtcgcgaccc
     7501 agagcgctgg cgcggtgatc gagagcgtga tgatcatgac gagcgcggtg tggcgcagcg
     7561 cgcgatcgag gagcggccga tacgctccct tgagcttgcg gatcagccaa acgtcctcgt
     7621 gtgcaccttc gcgtgtcggc ctgagcagac ctgccgcgac cacgggaacc agcgcgagcg
     7681 cgagcacgag cgccgcaagg acggccgcga tcacagcacc agcgagcggt ttgtacatgc
     7741 gaccttcgat gccagtcatc gcgaacagcg ggatgaacac ggcgatgacg accacggtgg
     7801 cgaatgcgat cggccgcgcg acttcgattg cggcagcgag cgcgttcccg tggcgatcgc
     7861 cctcgcgccg ttgcgagatg cggtggacga tattctcgac gacgatgatc gacgagtcga
     7921 cgagcaggcc gaccgcgatc gcgaggccgc cgagcgtcat cgtgttgatg ccaatgccgg
     7981 ccacgcgcat gaacaccccg gacagcgcga gagacagcgg caatgtgagc gtcacgatca
     8041 gtgccgcgcg cacattgccg agcagcacga acagcacgag cgcgacgagg caggcgccga
     8101 tcagcacggc gcgcccgacg ccgccgagcg acgattcgac gagctgggac tggtcataca
     8161 cggtacggat ctcgacgccc ctgggcaggc tggtcttgag gtccgcgatc gccgcccgga
     8221 tgccgcgcgc gaccgacacg gtatcggcgc caaactgctt cacgatgcgg cagctcacga
     8281 cctcgccatt caagcgatgc gcgatgccgc gtcggacagc cggtgcctcg cggacctcac
     8341 cgacatcgcc gagcaatacc ggtgtgccac ccttcatcgc gacgacggtg ctgcgtagtt
     8401 gctcgacgtt cgctgcacga cccgcagcgc ggacggtcca ctccatctgc gcttgcgtga
     8461 cgaacccgcc tgctgcgctg acgttcgcac cgcgcgccgc gtgcatgatg tcgtcgagcg
     8521 ttacgccgcg cgcagccatg cgatcgggat ccatctgaat ctggaactcg cgcaggtagc
     8581 cgccgagtcg ttccacagcg gcgacaccgg ggaccgcgag cagccggttc ttgatgtcat
     8641 tttccgcgag gtcgcgcagt gtcatcaggt cggcgatgcc gctgcgcgct tcgaccgtga
     8701 tctcgaagat ctcgttcagg cgaccggtca cgccggacag cagcggtgtc tcggtgccgg
     8761 gcggcagatc gacttgcggc aggcgctccg cgacgtactg tcgtgatcgc gagtagtcgg
     8821 catctggttc gaactcgatc gtgacctgaa cgacgccgag cgtcgatgac gaccgcacgc
     8881 gtcgcacctg cggcaggccg gagagcgcgg tctcgaacgg aatcgcgacg cgcatctcga
     8941 gctcgtccgc gttcatcgcg gagttctgca cgatgatgtt gaacaccggc gccgacagat
     9001 cggggaacac gtcgcgcggc agctcgcgca tccagaccgt gcccatcgcg gcgccagcga
     9061 gcgcgagcac gatcgcgatc aacggcttgc ggaagcttgc agtgatgaat ctctccagca
     9121 tggcgcctcc tcagtcctcg tgtccgcctg cgccgctggc cttgtcagcc tcggctttga
     9181 gcaggaaggc gccctcgacg acgacttcat cgccaggctg gagacccgag agaacctcga
     9241 cttcgcccga caggtcgcga ccacgaccga tcgaacgaac cgcgaactcg ccaggtccat
     9301 gcggaatgaa caccgcccag gtcgcgccga cgcgttgcac cgctgcagcg gggaccgtga
     9361 ccaccgtgtc cggcccgacc tcaccgagcg gaagcgcgac ggtggcggac atgccaggac
     9421 gcagcaagcc gtcggagttc ggtacgtcga gacgaacgtc gatcgtgcgc gatgcctgat
     9481 cgacctcgcg gccgatccac ttgatcgttg cttcggtcga cttgccgggc agggcggcaa
     9541 acgtggcggt cgcagtgcgg cccggctgga cacgcaccgc gtcgcgctcg aatacatgag
     9601 cgtggagcca cagcgtcgac aggtcgccga cgcggaacag cgtcttcgac ggatccgcga
     9661 gctgccccat gaccacgctg cgatcgatca cggtgccagc gatcggtgac ttcaacgcga
     9721 gcgcgctgtc gcccgacgcg ccgccgaact tctgcaacga cgaagcggtc accttgtacg
     9781 ctgcctcggc ctcggtcagc gccgcctcgg cttcgatcag ctcgcgctcg gggatgagtc
     9841 gatccgcagc gagcttgcgt ttgcgctcgg cgttcgcgcg cgcgacgtcg acgcgtgagt
     9901 gcgcagcacc gacttcagcg cgaccttgtg cgagctcggg gctcgcgagc tctgcgagca
     9961 cctggccggc cttcacggtg tcgccgggct tcgcgaggat cttggtcacg cgcgcactca
    10021 ccggcgcggc gatctccgca tacgcctctt cgttcacgtg cagctcgcca agcgccgaca
    10081 ccgtttcggc ggccgcgcga gcttgtgcct tgccggtggt gacgcgcagg tcgcgcatca
    10141 tgtccggcgc gatcttgatc atctcggggc cgtgttcgtg tgcgttcccg tgttccgccg
    10201 tgtccgagcc tgcgtgcccg gccggttccg gactcgtgta gtcatcttgg ttcggctcgg
    10261 tcttcttgcc gccacaaccg ccgataagga gcagcgcgag catcgtgagc ttgttcatgg
    10321 tgcacctccg gcgacgaacc gcgcagtggc cgcggctttc gctagcgcga gcaaccgttc
    10381 gaggtgctcg cgtcgtccgt cgagaatctg ttgacgcgcg acgaggtagt cgctgaccgc
    10441 gatctgcccg gcgtcgatcg tcttctgcag gagctgctcg ctgtcgtcga gtagcggaac
    10501 gacgtctgtc tcgaagcctt cgaccgctag acgcgcgctc gtatacgccg cgaaggcgtc
    10561 aacgctctcg cgccccgcgg ctcgcagtgt cgttgctcgc atgtcgctcg cgcgtcgctc
    10621 gcgcgcggtt gcggccgcct tctctccttg cgcgcggttc cacaatggaa atgtcatgcg
    10681 tagaccgccg agcacgatcg actgggtgtc ttcgcgctgg tagccggccc acagccacac
    10741 ctggggtagc ccgttcgcac tcgcttgcgc tcgttcggct cgcgcgacgc cgcgttcggc
    10801 atcgagcacg cggatatccg cgcgactccc tgcggttccg cgaagggtcg cgagatccgg
    10861 cagcgcggcc ggcttgagat cgccgcgcac cacgatcgca tcgtcgggtg tagcgccgat
    10921 cagtgcagcg agccgaccca ccgcgctcgc ttgatcggac tgtgcggact gcgtggctga
    10981 ccgcgcgcgc ccgagcgcag tcctcgcgag gtttgcgtcg aggtcggtaa tctcgccggc
    11041 cttgcgtcga cgatcggcga ccgcggcggc ccgctgggcg aagtcctcgg cgcgctttgc
    11101 tagctccgcg gctcgtaccg cgaacagggc atcgtagaac gccttcgtga cctcgagatc
    11161 gagctcgcgc aacatcgcgt cgagctcggc ctgcgtctgc gtgactcctg cacgtgcgag
    11221 ctgccggcgt ggcgacctcc ggccgagctc gaggttttgt tcgatccgcg cgtcgacatc
    11281 ggtcggtcgc gtgctgatca atcgcggccc ggcgccggcc tcgatctcgg ggttgtcgat
    11341 gaatggcagg ttggcaccag ttacggcggc gttcgcgatc gcgatctgac cgcgtgcacc
    11401 aacggccgca ggtgccgcct gatgcgcacg cgcgatcgca ttcggcagat cgagatcgat
    11461 ctggtcggcg gacgcgggaa gggaatgcag tacgagcgcg gctagcgcta gccgcgcgaa
    11521 cggaacctga gacatgacgc acctcgatca gcgacgctgc gatcgctcgc acgcgtcaga
    11581 acgacgtcgg ctcggaagtg aacgagtcag acgacgtgct tcgggatgtg caagatgtcg
    11641 gccgggtcgg ggctgccgtg catacggtcg gcgttgtcga acacaaccgg tgcggcttcg
    11701 gtaggtggtg gcatcgtcac cgacgcgatc gccggtggcg tttggctctg aacgctcggg
    11761 caatgacaag ggcacgatgg cgcgtcgttg cccgacgtgc agtcgttgcc gcatccgtcg
    11821 tcacggcact cgatctcgca tgcatgcctg cgcacgagct ccgcaattcc cgtcgcctgc
    11881 aacagggcga gaaccagcaa aagggcgcgc ggaaccgtca cctcttatga gtagcacgag
    11941 cgcaaaccgg gggaccaatg acacgctcgc gaccggcgac accgaacgcg tgtgaatgcg
    12001 tacgaggccc acacccccgc cgccggcacc acccaccgca gccacgaaat cgccaccacg
    12061 gccatcgtgg ccacgaccac catcgtcgac ggtggttggc cgtcagaccg acctgatacg
    12121 gattgcggat cgtggagcgc aacggaggtc gttgcagctc tgcatcgtga tgggagtttg
    12181 gaatgggtac gaaactgacg ccgcaggttc tggaggcgat tcgcgttgac cgcgaggagc
    12241 acgggttcac tctgacaagg atcatggagc gccatggttt gaggcgaacc actgcctaca
    12301 cggcgatcgc gggtctcgat gactcgaagg tgcagcgagc gtcaccgacc cgacgtgtcg
    12361 tgcaacccgt cgatgctccg ccgcggccgc cgctttccaa agccaacctc ggcgaggccg
    12421 cgcgccagct gatcgccgga cggatgctgc tcgctgggct cgacgtcttt caaccaatcg
    12481 gcgaggacac tccgatcgat ctactcgttc tgcgtcccga cggggtggcg ctgaagtgcc
    12541 aatgcaaggt catgtacatc gccaagacag gcgtgcacgt gatgcctttc tgcactattc
    12601 ggaaatgggg cccgaacgcg aaggtcgtgc ctcatcgcta cacccgagac gaagtcgact
    12661 tctttctcgg atatgcagtg gaggtcgacg cggtgttcgt cttcccgttc gacgccaccg
    12721 cacacttcaa gtcgtcgatg accgtctggc tcttgcgtga accggcaaac aacaacggaa
    12781 catcacgctt cgacgccgct ccctaccgga atgccttcca tcttctgagc gcgcaagacg
    12841 gacacccaac gctcgataag tgattgtgat cccaacaggg ctcgaacctg tggcctgagc
    12901 tttaggaaag ctccgctcta atccaactga gctatgggac cggaatcgtc cgcattactt
    12961 agcacgggtg gccggacgat ccgagtctgt cgtcgtcgat gtccttaggt accccaaagc
    13021 tcaggcgaag ccgcggatcg tgtggttcgg ccctggatca caagcacgcg ttgtagccga
    13081 agtcaggttt acgactcgtg gccaggtccg cccgccacgt tgcgatgtca cgctc
//
LOCUS       DASUFX010000335        14942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1857299, whole genome shotgun sequence.
ACCESSION   DASUFX010000335 DASUFX010000000
VERSION     DASUFX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857299"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFV99_13395"
     CDS             complement(<1..623)
                     /locus_tag="VFV99_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060354.1"
                     /translation="MVMLRLLSRGLLIAALSLGVVAVKAHPVYADDDDEEEDDGGDDG
                     GSTKSSGDEDGDEEEEADPDQPIVTAGGLFTMKSYPVREISRPLTMTQKITQVRVSAG
                     TDISAKGAFGSGGLSVEGIYGQADNFNIIGGLTNAYNFKQFGFYAGFEAALVYDILDI
                     RLAANLHRNALPVYKDFCNPPAMPNEMPVGGACMNASSAIQSLPSGE"
     gene            complement(918..1265)
                     /locus_tag="VFV99_13400"
     CDS             complement(918..1265)
                     /locus_tag="VFV99_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060355.1"
                     /translation="MGEKIAKLGIKRDNDMMYYIKSGDVWATPRKKPGQPKGKAAKVA
                     AAGIEMDYSKYLYYLDGDGDVARKERQVGGSKRKSSKKSAPKAKKGGAKKAAKKTAKK
                     AGKKGGGKKGKRK"
     gene            complement(1458..2348)
                     /locus_tag="VFV99_13405"
     CDS             complement(1458..2348)
                     /locus_tag="VFV99_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5060356.1"
                     /translation="MSARLSDDLIADLVDAGSREHYSDAALYDYEYRRRRADVQFYRE
                     LAKMRLPDGGRILELGAGSGRVTIALARAGYEVVALDTSKPMLDKLRGRVAALPKAVA
                     RRITVVQGDLRDFDVGETFPLVIAVFNVLEHLYTRVEVDACLRRVAAHLTQAKKSREG
                     GGAFAFDVQMPDLAWLLRDSTKRWAKTRFTDPTTGRAMFYSTNHDYDPVGQIALIRLY
                     YEPVDGKGPSRIVKLSQRKFFPAELEGLVAHAGLRMVERYGDFGWRSLDGSAESQVLV
                     CEHAISRRENAVSRRGFRRG"
     gene            complement(2416..3912)
                     /gene="pcnB"
                     /locus_tag="VFV99_13410"
     CDS             complement(2416..3912)
                     /gene="pcnB"
                     /locus_tag="VFV99_13410"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX5060357.1"
                     /translation="MPSLDRTAIDPDADRVVRKLTRAGYKAYLVGGCVRDLLVSRTPK
                     DFDVATSATPNEIKATFRNSRIIGRRFRLAHVFFGSKIIETSTFRANPRDEDDHDLLI
                     RRDNVFGTETEDARRRDFTINGLFYDVEREEVIDHVGGLADLDARLIRTIGDPDIRFQ
                     EDPVRMLRAIKFAARLSFGFDPATWRALLRWRGEISKCAPPRLLEEMHRLLRGGAARR
                     SFELLVETGVLAVLSPYLAGLLEGAGAPTTAPPIADTAPRPTVARIEYEDEDPAEHDP
                     DDPDEEEGSLPINGEPEAVEVAVELDADEREWHRVWADEPPARPAHAIAIKLSFLPDT
                     EVGKRRALVWEALSHLDVAVTDGRDPSNAVAFGILLAPFITSGELRGGELHGAINEIA
                     HPLIAQLHVTRRDSERLRYILMAQRKLAAARKRGGQAELAGGRELIDDAVLLYALLER
                     AAGNEGALPEPVAIAEGRDGDDEDDDPNRPRKRRRRRRGGRRRREATA"
     gene            4048..5487
                     /locus_tag="VFV99_13415"
     CDS             4048..5487
                     /locus_tag="VFV99_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5060358.1"
                     /translation="MPDKLTTAITDFETLHTPVAIFELDGTFVAINRASEALLGRAAA
                     EVVGHKTWEFAPGVEHIWDEMVAAARVRGTSRGTITLATPHEHRQIHYINTLRSYEGK
                     TYVLSYAFELPSGDAALEARHRAEALGLVAGGIAHDFNNQLVSVLAEAGIARENPSID
                     DETREAFRRIEAAAHRMAQLTRQLLAYAGRGRFVSEMLDPDGLVEDMREPLARLMRPD
                     ATLQITTSANRVALEADRTLLCQVIQNLVVNASEALGDGGGSIIVTTSHSGGSWFFEV
                     SDSGIGMDRHTVARIFDPFFTTKRDRHGLGLSAAQGIVRRLGGDISADSTPGGGSTLR
                     VSLPVVPGAQPVRRRTPTELVPVSTLRDVRVLVADDEPGVLSTVKRLLERRGAQVVIA
                     SDGAQARVRLAQGIYQIVLFDVMMPELTGYQLLPLARDLQPSAKVMLMSGYTEHARGH
                     GDEPDAFLEKPFTAKALDAAIDALIVPPR"
     gene            complement(5484..8861)
                     /locus_tag="VFV99_13420"
     CDS             complement(5484..8861)
                     /locus_tag="VFV99_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5060359.1"
                     /translation="MTDSRKPPSGHSDEEQTNVDPKRRVTDEERTAVDPKRPTKDATT
                     AVDPKPRAADSQSSIPVEVPRRNVDRFARPPEYPRHKSPAVEPAQDDPATQPISMSPE
                     LPSSPLAVTREDKTPIRDEAGTKPFELLPPRTAATPATGIPKADDLDPSTRPLDLPRR
                     APASNPVVTVEMTEQNTGVLSVTEDSRVPAPRLSHTAEISAAIGALIDDEQPSQPGIT
                     VEMVEENTGVLNTTKAEDTAAQKIVTDADPPARPLSAAARKIEADKKAAADKATIEKV
                     AAKVAAEHAAGKAAEQAATKAAAEKAAAKAAAEKAVESVIAADKHRAATAADDKAKKG
                     SDPISAAGSDPISAVAAEDIAESTLASDDKPDEEMVAWVAPDDGFQLADEIGSAPAPL
                     VPNPPILPSPQEAALEAARIVSQMVDIESESGAVIAAAAAAGAAIEAAKAAQKASIDP
                     RASQPSMRIPPELTAAQAQSHDPITTVRLSRDRILAALADEDIAVPVPTARDGRMPRA
                     RTEPGAIRARTEPGIEDDGVYEEFDPVAIAAAAIGRLRARARTDLAELRVHYHRHDIL
                     VLLLALVIIIVAGRIHASMVTPPSDHFSERGLTFDHPATWLKPEPVPPIAPRLVRDPT
                     GAATKDDGLYHVELSSVGSTAKLEVLIDKKPTWSNIVTGLELDRRSRWGELYTLDASS
                     VKSIAGHDWLRTAYRYAHVVDKGDVPRVDHAVELATVDRDQLYVVTIYGTPLELDQIE
                     DVVSPSLRVPSQTGLPLVPQTSRLSQRQFPSPVGRAFDSTVMVVVADLVDGRLKARGG
                     GSGVIVGAEGSILTNYHVIHDKDGRLHDVFIIGRFSAPDRAPQLQCAGRPTRSKLQRD
                     LDLALIKCDMDLDGRTWTPTSSGIWPTLPEAKASDVKIGQRLWVLGYPDVGGGGLQLG
                     VAEVEGWTGEDGAQGKDFIKTNGSITHGNSGGPVIDDQGKLVGIASAFRTKVTASGGV
                     VEMAQVGLVRPLQTASNVLAYAAAGWTPREGYTDVELTPTAVEAAAEGIRIFTTVIDD
                     ANEQAVRDATVMVLKSGVSTSSIDMNRLDDQAIAWGKTNAQGEVRLKQLVPVPGTYTV
                     MVVAPGYEPLIGENELHLDTNTPPSFDPWGKIGLRSR"
     gene            complement(8858..9742)
                     /locus_tag="VFV99_13425"
     CDS             complement(8858..9742)
                     /locus_tag="VFV99_13425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX5060360.1"
                     /translation="MKRPRWSLRRARVLVGTKFLLVAGLVGFVVVCWLGELVAGLDRP
                     VHLGPAFAAFMSCVPAMFWLGFFYLMDRHEPEPKQLVVGVCVLGALVAAPLADFVLSQ
                     AVPPLGLEQQGLSAFSLDRVLYAVLVIGLTQEMCKYAVVRYTIYMSREFDEPMDGIVY
                     MMACGTGFAVWVNYHRLSGQGHQVYLSTGAAQAVVTTLAHASFAGALGYVMGRAKFSR
                     RSAPVRGALLMGGLLGAALLNGQFSVVEAWVQQVGFAQHPWRGVGYAAACAMAVFAVI
                     WFASQKLLQSSPFKDKPA"
     gene            9845..10669
                     /locus_tag="VFV99_13430"
     CDS             9845..10669
                     /locus_tag="VFV99_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5060361.1"
                     /translation="MLTIHELAVYLHLDEATVNKLVAGGKIPSIQLERQWRFKKAAID
                     EWIEQQLVGDDENFTDVPDGMKLPLEDLLPDQAIITNLRATTPIAVIEELAARAYTNG
                     WLADKPWFVGAVVERESLASTAMEGGVAFLHTRAKDKGKIARPFLVVGRSWSGIPFGA
                     VDGNPTYLFFLMGLKYDRLHLPILGRLARALRNPATIAKLRSMQSPDQMRALLLKEDA
                     NARAGLVPEFQAEAFKPKLDRTLRLRAIRRLQQQKKPEPEPPPPSKRGKAKAKPAK"
     gene            10745..11428
                     /locus_tag="VFV99_13435"
     CDS             10745..11428
                     /locus_tag="VFV99_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060362.1"
                     /translation="MGRTALYRDPVPYRPARANVQLDPDALILVLPDSWIRRHPLDGM
                     QITISDGFVSERSARGSSESIITRHGAPPAPPRVLRRFVRMLILERDGERIVVITPPD
                     HGAVAPNVVQMPEAPSDAAIIDELAWDALAEWLLGGGRLAACSIADLARLACIATPQF
                     AGLIGEVAAQRALEHVWAASGPLRGGGDLDSVLQPLVDAAKHSSRASEALVSALSHAA
                     GASRRRRWR"
     gene            11467..11802
                     /locus_tag="VFV99_13440"
     CDS             11467..11802
                     /locus_tag="VFV99_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060363.1"
                     /translation="MRLAILATLALGLAACQSSDVSRELGARCDNSNDCDVRCLSGAD
                     WPGGFCTIACETDVDCQADASCLEEEGGVCAFTCVSDAGCQFLGQGYTCKERDARGGA
                     GKRMVCRGG"
     gene            11787..12431
                     /locus_tag="VFV99_13445"
     CDS             11787..12431
                     /locus_tag="VFV99_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060364.1"
                     /translation="MSRRLAIVLLFAPAAAAADDTAAPGQDSATTATAETGNPSAPAD
                     MNDQAISATLGVAFGGRTTPGGLAVAGHYFYQLSDHDWFDGSAQFVFGSGGAECFRDR
                     MNDTICDHGLADGYAGGVGAGVRRFLPALASGDFWPFARAGLAAAVVRFPDDETTGIT
                     LDAQLGAGMRVAVSEAIAITAQFDFVLGLGRFSNGVGGEPQLGVMVTAGAEFRL"
     gene            12428..14020
                     /locus_tag="VFV99_13450"
     CDS             12428..14020
                     /locus_tag="VFV99_13450"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5060365.1"
                     /translation="MRWLAIAALAIACGKPAKPRSTTTLAVSVTDNGKPVGARVLLFD
                     AQNQPVHIGTIDLFGRRQGAGACSIAPGVLGTWDGLILAYGVGEVPIGRDACVPSPAI
                     QYGRYHVWAWRGIEYEKWEGDVDLSANRGRVELKIALDRAWTPQGTLAADLHVHAHAS
                     DDSRMPNPQRVIAQVAAGIQVLALTDHNWNGDVDAEIHDLELDDQIASIAGNELTANA
                     LHVGVYPVPFDRNVPRGGSPSHDKVVPASPQEVFAIAHSFTTGPIVQVNHPRFRVNSL
                     YDTVGWNGVSWPPPFPLAFDAVEVIAGYSAFNVDGDRRLDDGVRDFYTFIDHGHLITG
                     VGNSDTHDFNWVLDGTARNYVYVDDPRVYPFDEPGFIAALRARRVVATTGPWLDVEAA
                     VKKDATPTVGPGGWLVANDGAIWLDVTLQQARWVKTERIRITIGSPEGPKLVQTIDVP
                     PNVREFRWAGRIDVGAQDTWVGVTADGDTAMPLEFTGTYQRDKWQHAGVTPMALISPI
                     LVDADHDGRWKRGDADVPLGVR"
     gene            14056..14622
                     /locus_tag="VFV99_13455"
     CDS             14056..14622
                     /locus_tag="VFV99_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5060366.1"
                     /translation="MEAFVQGWSLTIALCGFIVLVFVAGWLHVHRELAWVKALTDHLG
                     DELVSAAPLKPRSLELQALRDEAHAILTAKDPAWAQKQVRSWQMRAQRLEPVLAFWVD
                     LLRQLGLLGTVLGIGLSLMYTGSDLTKLLGPLALKVWTTVAGLACSIALSASFSMKLT
                     AWVDAAEKNLEAWDARRHAGTVKAEDMK"
     gene            14619..>14942
                     /locus_tag="VFV99_13460"
     CDS             14619..>14942
                     /locus_tag="VFV99_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060367.1"
                     /translation="MRRARRHVRVPPDLTSLFDVLFIVIFAALIRAAAVEKAAAQAAT
                     PKPVAPAPPVKLANAQLHEKALADLGKQLAARPYVVVRVSERAIKALETGDNVQKLDT
                     PLLEHS"
BASE COUNT         2377 a         4924 c         5056 g         2585 t
ORIGIN      
        1 tactcgccac tcggcagcga ctggatcgcc gagctcgcgt tcatgcacgc gccgccgacc
       61 ggcatctcgt ttggcatcgc gggcgggttg cagaagtctt tgtagaccgg cagcgcgttg
      121 cggtggagat tcgccgcgag gcgaatgtcg aggatgtcgt agaccagcgc ggcttcgaag
      181 ccggcgtaaa acccgaattg cttgaagttg tacgcgttgg tgaggccacc gatgatgttg
      241 aaattgtcgg cctggccgta aatgccttcg accgacaacc cgccgctgcc gaacgcgccc
      301 ttcgcgctga tatcggtgcc ggccgacacg cgcacctggg tgatcttctg cgtcatcgtc
      361 agcggccgcg agatctcgcg caccgggtac gacttcatcg tgaacagacc gcccgcggtc
      421 acgatcggct gatcggggtc tgcctcttct tcttcgtcac cgtcctcgtc gccgctgctc
      481 tttgtgctgc caccgtcgtc gccgccgtcg tcctcttctt cgtcgtcgtc gtcagcgtag
      541 acggggtgcg ccttgacggc gacaacaccc aagctaaggg ccgcgatcag cagccccctc
      601 gatagcaggc gaagcatcac cacctctcgg aaatgctaca gcaaacgacc gccgaacggt
      661 gaagggcagg ggggcccaaa ttggcgaggc ggcgcgcgcc gatactgcac catcgctacg
      721 gccccagccg tacccgacaa cgtgacggcc ctgtggttgt cgacgggagc acaggcgttc
      781 gcgaaacatc gaccggcagg ttgatctgcc cagcgcggct gttgcccagc gcggcggttc
      841 cgaaaaatcg aaaggcccgt tggtcaccca acgagccctt cgcgttctaa tctaacgagt
      901 cgaagcgcga gcggaaatta cttccgcttg cccttcttgc cgccgccctt cttgccggcc
      961 ttcttggccg tcttcttggc ggccttcttg gcgccgccct tcttcgcctt gggcgcggac
     1021 ttcttgctgc tcttgcgctt gctgccgccg acctggcgct ccttgcgcgc cacatcgccg
     1081 tcaccgtcga gatagtagag gtacttgctg taatccatct cgatgccggc cgcggcgacc
     1141 ttcgccgcct tgcccttcgg ctgaccgggc ttcttgcgcg gcgtggccca aacgtcgcca
     1201 ctcttgatgt agtacatcat gtcgttgtca cgcttgattc cgagcttcgc gatcttctca
     1261 cccatgattt gagaccctcc ctagggataa ttgtgggtgc cgcgaacgct acacgaaccg
     1321 tctgacaaac actcccgaaa acctcgtcga aaaatcgttt tttttcgttt tttctcgacg
     1381 gagatccgga caggcgccga agtgatcggg attctcgacg aggaaaagct gtggataagt
     1441 tgtttctcgt cgaggatcta accgcgccta aatcctcgtc gagaaacggc attttctcga
     1501 cgagaaatcg cgtgctcaca gacgaggacc tggctctctg cgctcccgtc gagggatcgc
     1561 cacccgaagt caccgtagcg ttccaccata cgtagccctg cgtgagcgac gaggccttcg
     1621 agctcggccg ggaaaaattt gcgctgcgag agcttcacga tccgggacgg gcccttgccg
     1681 tcgacgggct cgtagtagag gcgaatgagc gcgatctggc cgacgggatc gtagtcgtgg
     1741 ttcgtcgagt agaacatcgc gcggccggtc gtcggatcgg tgaaacgggt cttcgcccag
     1801 cgcttcgtcg agtcccggag cagccaggca aggtcgggca tctggacgtc gaacgcgaac
     1861 gcgccgccgc cctcgcgtga tttctttgct tgcgtcaggt gagctgccac acgccgcaaa
     1921 caggcatcga cctcgacgcg cgtgtagagg tgctcgagga cgttgaatac ggcgatgacg
     1981 agcgggaacg tctcgccgac gtcgaagtcg cggagatcac cctgcacgac cgtgatgcgc
     2041 cgcgcgactg ccttcggtag cgctgcgacc cgcccgcgca gcttgtcgag catcggcttc
     2101 gaggtgtcga gcgccacgac ctcgtagccg gcgcgcgcga gcgcgatcgt gacgcggccc
     2161 gagccggccc cgagctccag gatgcgcccg ccgtcgggca gccgcatctt cgcgagctcg
     2221 cgatagaact gcacgtcggc gcggcggcgg cggtactcgt agtcatagag cgcggcgtcc
     2281 gaatagtgct cgcgcgagcc ggcatctacg agatccgcga tcaggtcgtc tgataaccga
     2341 gcactcatgt gttccggctc tggccgaagt cgatactgcc cagcccgaag tccgaagccc
     2401 gaggtccgaa gcccgtcacg ccgttgcttc gcgtcgcctg cgtccaccgc gtcgtcgccg
     2461 ccggcgtttg cgcggacgat tcggatcgtc gtcttcgtcg tcgccgtcgc ggccttccgc
     2521 gatcgcgacc ggctcgggca gtgcgccctc gttgccggcg gcgcgctcga gcagcgcgta
     2581 cagcaggacc gcgtcgtcga tgagctcgcg gccaccggca agctcggcct gaccaccgcg
     2641 cttgcgcgcc gccgccagct tgcgctgcgc catcaggatg tagcgaagcc gttcgctatc
     2701 gcgccgggtg acgtgcagct gtgcgatcag tggatgggcg atctcgttga tcgcaccgtg
     2761 cagctcgccg ccgcgcagct cgccggacgt gatgaacggc gcgagcagaa tgccgaacgc
     2821 gaccgcattc gacgggtcgc ggccatcggt gacagcgacg tcgaggtggc tgagcgcctc
     2881 ccacacgagc gcgcggcgct tgccgacctc ggtatcgggc aagaacgaca gcttgatcgc
     2941 gatcgcgtgt gcggggcgtg ccggcggctc gtcggcccac acccgatgcc attcgcgctc
     3001 gtccgcgtcg agctccaccg cgacctcgac ggcctcgggc tcgccgttga tcggcagcga
     3061 cccttcttct tcgtcgggat cgtcgggatc gtgctcggcc ggatcctcgt cttcgtactc
     3121 gatgcgtgcg accgtcgggc gcggcgcggt gtcggcgatc ggcggcgcgg tcgtcggtgc
     3181 acccgcacct tcgaggaggc ccgcgaggta cggcgatagg actgcgagca caccggtctc
     3241 gacgagcagc tcgaaggagc ggcgcgcggc accaccacga agcaggcggt gcatctcttc
     3301 gagcaggcgc ggcggtgcgc acttgctgat ctcgccgcgc cagcggagca gtgcgcgcca
     3361 ggtcgcggga tcgaagccaa acgacagccg cgcggcgaac ttgatcgcgc gcagcatgcg
     3421 gaccggatct tcctggaagc ggatgtcggg atccccgatc gtgcggatca ggcgcgcgtc
     3481 gaggtcggcg agcccaccga cgtgatcgat cacttcttcg cgctcgacgt cgtaaaacag
     3541 tccgttgatc gtgaagtcgc ggcggcgcgc gtcctcggtt tcggtaccga acacgttgtc
     3601 gcggcggatc agcagatcgt gatcgtcttc gtcgcgcggg ttcgcgcgga acgtcgaggt
     3661 ctcgatgatc ttcgagccga agaacacgtg cgcgaggcgg aagcggcggc cgatgatccg
     3721 agaatttcgg aacgtcgctt tgatctcgtt cggcgtggcg ctcgtcgcga catcgaaatc
     3781 tttgggcgtg cggctgacga gcaggtcgcg cacgcatcca ccaaccagat aggccttgta
     3841 gcccgcgcgg gtgagcttgc ggaccacgcg atcggcatcg ggatcgatcg cagttcggtc
     3901 cagtgacggc atcgtaacga tgcagccgta ccatgccggg caaacaaggg gcaactacgc
     3961 aggtctattg agctaccccg gtctttaccc gagccctgcc gaccggcgtg atgatatgga
     4021 ccgattacga caacgattac gagtcccgtg cccgacaagt taaccaccgc gatcacggac
     4081 ttcgagaccc tgcacacgcc ggtcgcgatc ttcgagctgg acggcacgtt cgtcgccatc
     4141 aaccgcgcca gcgaggcgct gttggggagg gccgccgccg aggtggtcgg ccacaagacc
     4201 tgggagttcg ccccgggcgt cgagcacatc tgggacgaga tggtcgcggc cgcccgggtc
     4261 cgcggcacgt ctcgcggcac gatcacgctc gcgaccccgc acgagcaccg ccagatccat
     4321 tacatcaaca cgctacgcag ctacgaaggc aagacctacg tgctgtcgta cgcgttcgag
     4381 ctgccatcgg gcgacgcggc gctcgaggca cggcatcgcg ccgaagcgct cggtctggtc
     4441 gccggcggta tcgcgcacga cttcaacaac cagctcgtca gcgtgctcgc cgaggctggt
     4501 atcgctcgcg agaacccctc catcgacgac gagacgcgcg aggcgttccg ccgcatcgag
     4561 gctgctgcgc accgcatggc acagctgacg cgccagctgc tcgcgtacgc cgggcgcggc
     4621 cggttcgtca gcgagatgct cgatcccgat ggtctcgtcg aggacatgcg cgagccactc
     4681 gcgcgtctga tgcgtccgga cgcgacgctc cagatcacga ccagcgcgaa ccgcgtcgcg
     4741 ctcgaggccg accgcacgct gctctgccag gtgatccaga acctcgtcgt caacgcgtcc
     4801 gaggcgctcg gcgacggcgg tggttcgatc atcgtcacga cgagccattc aggcggcagc
     4861 tggtttttcg aggtgagcga cagcggcatc ggtatggatc gccacaccgt ggcgcgcatc
     4921 ttcgatccgt tcttcacgac gaagcgcgat cgccacggcc tcgggttgtc ggctgcgcaa
     4981 ggcatcgtgc gccggctcgg cggcgacatc agcgccgaca gcacgcccgg cggcggcagt
     5041 acgttgcgcg tcagcctgcc cgtggtgccc ggtgcccagc cggtgcgccg acgtacgccg
     5101 accgagctgg tcccggtgag cacgctacgc gacgtccgcg tcctcgtcgc cgatgacgag
     5161 ccgggcgtgc tgtcgacggt gaagcggctg ctcgagcggc gcggcgcgca ggtcgtgatt
     5221 gcatccgatg gggcgcaggc gagagtgcga ctcgcgcagg gcatctatca gatcgttctg
     5281 ttcgacgtga tgatgcccga gctcaccggc taccagctgt tgccgctcgc gcgcgacctc
     5341 cagcccagcg cgaaggtgat gttgatgtcg ggctacaccg agcacgcgcg cggtcacggc
     5401 gacgagccgg atgcgttcct cgagaagccg ttcacggcga aggcactcga cgctgcgatc
     5461 gacgcgctga tcgtgccgcc gcgctagcgg gagcgcagcc cgatcttgcc ccacgggtcg
     5521 aagctcggcg gcgtgttggt gtcgaggtgg agctcgttct cgccgatcag tggctcgtag
     5581 ccgggcgcga cgaccatcac ggtgtacgtg ccgggcaccg gcaccagctg cttgagccgc
     5641 acttcgccct gggcgttcgt cttgccccat gcgatcgcct gatcgtcgag gcggttcatg
     5701 tcgatcgatg acgtgctgac gcccgacttg agcaccatca cggtcgcgtc gcgcactgcc
     5761 tgttcgttgg catcgtcgat caccgtcgtg aagatgcgaa tgccctcggc ggcggcttcg
     5821 accgcggtcg gggtcagctc gacgtcggtg tagccttcgc gcggcgtcca gccggcggcg
     5881 gcgtacgcga gcacgttgct cgcagtctgc agcggacgca cgagcccgac ctgcgccatc
     5941 tcgacgacgc caccgctcgc ggtgaccttc gtgcggaatg ccgaagcgat accgacgagc
     6001 ttgccctgat cgtcgatcac cgggccaccg gagttgccgt gcgtgatcga gccattcgtc
     6061 ttgatgaagt ccttgccctg cgcaccgtcc tcgccggtcc agccttcgac ctcggcgacg
     6121 ccgagctgca gcccgccacc gccgacatcg ggatagccga gcacccacag ccgctggccg
     6181 atcttcacgt cggaggcctt cgcctcgggc aacgtcggcc agatgccgct gcttgtcggc
     6241 gtccaggtgc gcccgtcgag atccatgtcg cacttgatga gtgcgagatc gagatcgcgc
     6301 tggagcttgc tgcgggtcgg tcgtcctgcg cactgcagct gtggcgcgcg gtcgggtgcg
     6361 ctgaagcggc cgatgatgaa cacgtcgtgg aggcggccgt ccttgtcgtg gatgacgtga
     6421 tagttcgtca ggatcgagcc ctcggccccg acgatgacgc cggagccgcc gccgcgcgcc
     6481 ttgagtctgc cgtcgacgag atcggcgacg acgaccatca ccgtcgagtc gaacgcgcgg
     6541 ccgacggggc tcggaaattg gcgctggctc aggcggctcg tttgcggcac gagtggtagg
     6601 ccggtctgcg acgggacgcg caacgatggc gacacgacgt cttcgatctg gtcgagctcg
     6661 agcggcgtgc catagatcgt gacgacgtag agctgatcgc gatcgacggt cgcgagctcg
     6721 acggcgtggt cgacgcgcgg cacgtcgccc ttgtcgacga catgcgcgta gcgataggcg
     6781 gtgcgcaacc agtcgtggcc tgcgatcgat ttcaccgagc tggcatcgag cgtgtagagc
     6841 tcgccccagc gcgatcgccg atcgagctcg aggccggtaa cgatgttcga ccaggtcggc
     6901 ttcttgtcga tcagcacctc gagcttggcg gtcgagccga ccgacgacag ctcgacgtga
     6961 tacaggccgt catccttcgt cgcagcgccg gtcggatcgc gaacgagacg cggtgcgatc
     7021 ggcggtactg gctcgggctt gagccacgtc gccgggtggt cgaacgtcag gccgcgctcg
     7081 ctgaagtgat cgctcggcgg cgtcaccatg cttgcgtgga tgcggcccgc gacgatgatg
     7141 atcacgagcg cgagcagcaa tacgaggatg tcgtggcggt gatagtggac gcgaagctcg
     7201 gcgaggtcgg tgcgcgcgcg tgcacgaaga cggccgatcg ccgcggccgc gatcgcgacc
     7261 ggatcgaact cctcgtagac gccgtcatcc tcgatgccag gctcggtgcg cgcgcggatg
     7321 gcgccgggct cggtcctcgc acgcggcatg cggccgtcgc gcgctgtcgg cacgggcacc
     7381 gcgatgtcct cgtcggcaag tgcggcgagg atccgatcgc gcgagaggcg aaccgtcgtg
     7441 atcggatcgt ggctctgcgc ctgcgccgcc gtgagctcgg gcggaatgcg catgctcggc
     7501 tgcgacgcgc gcggatcgat gctggccttt tgcgcggcct tcgcggcttc gatcgcagca
     7561 ccggcggccg ccgcggcggc gatcaccgcg ccggattcgc tctcgatgtc gaccatctgg
     7621 ctgacgatgc gtgcggcctc gagcgctgcc tcttggggcg acggcaggat gggcggattg
     7681 gggaccagcg gcgcgggcgc agagccgatc tcgtcggcga gctggaagcc gtcgtcgggc
     7741 gcgacccacg cgaccatctc ttcgtcgggc ttgtcgtcgc ttgcgagcgt gctctcggcg
     7801 atgtcctcgg cggcgacggc agaaatgggg tcagacccgg ccgcggaaat ggggtcagac
     7861 ccctttttcg ctttgtcgtc ggccgccgtt gcagcgcggt gcttgtcggc ggcgatcacg
     7921 gactcgacgg ccttctcggc ggcggccttg gcagcggcct tctcggcggc ggccttcgtt
     7981 gcggcctgct cggcggcctt gccggcggcg tgctcggcgg cgaccttggc tgcgaccttc
     8041 tcgatggttg ctttatcggc ggcagccttc ttgtcggctt cgatcttgcg cgccgccgcc
     8101 gatagcggac gtgcgggcgg atccgcgtcg gtcacgatct tctgcgcggc ggtatcctcc
     8161 gccttcgtcg tgttgaggac gccggtgttc tcctcgacca tctcgacggt gatgcccggt
     8221 tgcgagggct gctcgtcgtc gatcagcgcg ccgatcgcgg ccgagatctc cgcggtgtgg
     8281 ctgaggcgag gggccggcac ccgcgagtcc tcggtgaccg acagaacgcc ggtattctgc
     8341 tcggtcatct cgacggtgac gaccgggttc gatgctggtg ctcgcctcgg tagatcgagg
     8401 ggtctcgtcg atgggtcgag gtcgtcggcc ttcggaatgc cggtcgccgg cgtcgccgcg
     8461 gtacgtggcg gcaacagctc gaacggcttc gtacctgcct cgtctcggat cggggtcttg
     8521 tcttcgcggg tgaccgcgag tggcgacgac ggcagctcgg gcgacatcga gatcggctgc
     8581 gtcgccggat cgtcctgggc aggctcgacg gccgggcttt tgtggcgcgg atactccggc
     8641 ggacgcgcga accgatcgac attgcgacgc ggaacctcga cagggatcga cgattgcgaa
     8701 tccgccgcgc gcggcttcgg atcgaccgca gtcgtcgcgt ccttcgtcgg tcgcttcgga
     8761 tcgacggcgg tccgttcctc gtcggtgacc cgtcgctttg gatcgacgtt ggtctgctct
     8821 tcgtcgctat ggcccgacgg cggcttacgg ctgtcggtca cgcgggtttg tccttgaacg
     8881 gcgacgactg caacagcttc tgcgatgcga accagatcac cgcaaacacc gccattgcac
     8941 acgccgccgc gtagccgacg ccgcgccacg ggtgctgcgc aaatccaact tgctgcaccc
     9001 aggcttcgac gacgctgaat tgtccattga gcagagcggc accgagcaaa ccgcccatca
     9061 atagcgcgcc gcgcacgggt gcgctgcgcc gcgaaaactt cgcacgcccc atcacgtagc
     9121 cgagcgcgcc cgcaaatgat gcgtgggcca gcgtcgtgac gacggcctgg gcggcacccg
     9181 tggacaggta gacctggtgc ccctggcccg agaggcggtg gtagttcacc cagaccgcga
     9241 agccggtgcc gcacgccatc atgtagacga tgccgtccat cggctcgtcg aactcgcgcg
     9301 acatatagat cgtgtagcga acgacggcgt acttgcacat ctcctgggtg aggccgatga
     9361 cgagcaccgc gtagagcacg cgatcgaggg agaacgcaga caggccttgc tgctcgagcc
     9421 cgagcggcgg caccgcctgc gagagcacga agtcagcgag gggcgccgcg acgagcgcgc
     9481 cgaggacgca gacaccgacg acgagctgct tgggctcggg ctcgtggcgg tccatcaggt
     9541 agaagaagcc gagccagaac atggccggta cgcacgacat gaatgccgca aacgcggggc
     9601 ccaggtggac gggccgatcg aggcctgcca ccagctcgcc gagccagcag accacgacga
     9661 agccgaccag gccggcaacc aggaggaact tggtcccgac cagcacacgg gctcggcgga
     9721 gcgaccagcg cggtctcttc acgtcgaaat tctacggcac acgaggttgg cgcgcgcgtt
     9781 cacagccctc tagaatgccg cgcgagtcgt ttcgatgcca aaaaagcagg gttccgacgc
     9841 acagttgctg acaatccacg agctggcggt ctatctccac ctcgacgagg cgaccgttaa
     9901 caagctcgtt gcgggtggca agattccttc gatccagctc gaacgccagt ggcggttcaa
     9961 gaaagcggcg atcgacgagt ggatagagca gcagctcgtc ggcgacgacg agaatttcac
    10021 cgacgtaccc gacggtatga agctgccgct cgaggacctg ctccccgacc aggcgatcat
    10081 cacgaacctc cgtgcgacga cgccgatcgc ggtgatcgaa gagctggcgg cgcgtgcgta
    10141 cacgaacggc tggctcgccg ataaaccgtg gtttgtcggt gctgtcgtcg agcgcgagtc
    10201 gctggcatcg acggcgatgg agggcggcgt ggcgttcttg catacgcgcg ccaaggacaa
    10261 gggcaagatc gcacggccgt tcctggtcgt cggtcggtcg tggagcggca tcccgttcgg
    10321 cgccgtggac ggcaatccga cctatctgtt cttcctgatg ggcctcaagt acgaccggct
    10381 ccacctgccg atcctcggcc ggctcgcgcg tgcgctacgt aatccagcga cgatcgcgaa
    10441 gctgcgctcg atgcagtcac ccgaccagat gcgtgcgctc ctgctcaagg aagacgccaa
    10501 cgcccgcgcc gggctcgttc ccgagttcca ggccgaggcg ttcaagccga agctcgacag
    10561 gacgctccgg cttcgtgcga ttcgtcgtct gcaacagcaa aagaagccag aaccggaacc
    10621 gccgccaccc tccaagcgtg gcaaggcgaa ggccaagccc gcaaagtgac tggacgcgct
    10681 gctttgttag cgagtgcgtg acttcggtac gatcgcttcg ggagtgggct gctaagatct
    10741 cattgtggga cgtactgccc tgtaccgcga tccggtgccg taccggccgg cgcgcgcgaa
    10801 cgtgcagctc gatcccgacg cgctgatcct ggtgctaccg gacagctgga tccggcggca
    10861 tccgctcgac ggcatgcaga tcacgatcag cgatggcttc gtttcagagc gcagcgcacg
    10921 cggcagctcg gagtcgatca tcacgcgtca cggcgcgccg ccggcaccac cgcgcgtcct
    10981 gcgtcgcttc gtgcggatgt tgatcctcga gcgtgatggc gaacgcatcg tcgtgatcac
    11041 gccgcccgat catggtgcgg tcgccccgaa cgtcgtgcag atgcccgagg cgccgagcga
    11101 tgctgcgatc atcgacgagc tcgcatggga tgcactcgcg gagtggctgc tcggcggcgg
    11161 tcgtctcgct gcatgctcga tcgcggatct cgcgcgcctc gcgtgcatcg cgactccgca
    11221 attcgccggt ttgatcggtg aagtcgcggc gcaacgtgct ctcgagcacg tgtgggctgc
    11281 atctggaccg ctgcgcggtg gcggtgatct cgattcggta ttgcaaccgc tcgtcgacgc
    11341 agcgaagcac tcgtcgcgcg cgtcggaggc gctggtttcg gcgctgtcgc acgccgcggg
    11401 cgcgtcgcgt cgtcggcgct ggcgttagtg gcgcgacgga tcatcgtgcg gtagcttcgc
    11461 tcgcgaatgc gtctcgcgat cctcgcgact ctcgctttgg ggctggccgc gtgccagagc
    11521 tcggatgtct cgcgcgagct cggtgcacgc tgcgataact cgaacgactg cgacgtgcgc
    11581 tgtctgtccg gcgcggactg gcccggtggg ttctgcacga tcgcgtgcga gaccgatgtc
    11641 gattgtcagg cggacgcttc gtgcctcgag gaagagggcg gtgtctgcgc gttcacgtgc
    11701 gtcagcgatg cgggctgtca gttcctcggt cagggctaca cctgcaaaga acgcgacgcg
    11761 cgcggcggcg ccggaaagcg aatggtatgt cgcggcggct agcgatcgtg ctgctcttcg
    11821 cgcccgctgc cgcagcggcg gatgacacag cagcgcccgg tcaagactct gctacgaccg
    11881 cgacggcaga gaccgggaac ccgagcgcac cggccgacat gaacgatcaa gcgatcagcg
    11941 ccacgctcgg tgtggcgttc ggcggacgca cgacgccggg cgggcttgcg gttgccggtc
    12001 actacttcta tcagctcagc gatcacgact ggttcgatgg ctcggcgcag ttcgtgttcg
    12061 gcagcggcgg tgccgagtgc ttccgcgatc gcatgaacga cacgatctgc gatcacggcc
    12121 tcgcggacgg ctacgcgggt ggcgtcggcg ccggggtccg ccgcttccta ccggcgctcg
    12181 caagtggaga cttctggccg ttcgcacgcg caggtctcgc cgctgcagtc gtccgcttcc
    12241 ccgacgacga gacgaccggc atcacgctcg atgcgcagct cggcgcgggc atgcgggtgg
    12301 cggtttccga ggcgatcgcg atcaccgcgc aattcgactt cgtgctcggt ctcggtcggt
    12361 tcagcaatgg agtcggcggc gagccgcaac tcggcgtgat ggtcacggcg ggcgccgaat
    12421 tcaggctatg aggtggctgg cgatcgcagc gctggcgatc gcatgcggca agccagcgaa
    12481 gccgcggtcg acgacgacac tcgcggtcag cgtcacggac aacggaaagc cggtcggcgc
    12541 gcgcgtgctg ttgttcgacg cccagaacca gccggtgcac atcggcacga tcgatctgtt
    12601 cggtcgccgg caaggcgcag gtgcgtgctc gatcgcgccc ggcgtgctcg gcacgtggga
    12661 cgggttgatt ctcgcgtacg gcgtcggcga agtgccgatc ggccgcgacg cgtgcgtgcc
    12721 gtcgccggcg atccagtacg gccgctatca cgtgtgggcg tggcgcggca tcgagtacga
    12781 gaaatgggaa ggcgacgtcg atctgtcggc gaatcgcggc cgcgtcgagc tgaagatcgc
    12841 gctcgaccgc gcgtggacac cgcagggcac gctcgcggcg gatctccacg ttcatgcaca
    12901 tgcgtccgac gactcgcgca tgccgaatcc gcaacgcgtg atcgcgcagg tcgccgctgg
    12961 catccaggtg ctcgcgctca ccgatcacaa ctggaacggc gacgtcgacg ccgagatcca
    13021 cgacctcgag ctcgacgacc agatcgcgtc gatcgccgga aacgagctca ccgcgaacgc
    13081 tctgcatgtc ggcgtttacc cggttccgtt cgaccgcaac gtgccccgtg gtggttcgcc
    13141 gtcgcacgac aaggtcgtcc ctgcgtcacc gcaggaagtg ttcgcgatcg cgcacagctt
    13201 cacgaccgga ccgatcgtgc aggtgaacca tccgcgcttt cgcgtcaact cgctgtacga
    13261 caccgtcggc tggaacggcg tgtcgtggcc gccgccattc ccgctcgcgt tcgatgccgt
    13321 cgaggtgatc gccggctaca gcgcgttcaa cgtcgacggc gatcggcggc tcgacgacgg
    13381 cgtgcgcgac ttctacacgt tcatcgatca cggccacctc atcaccggcg tcggcaacag
    13441 tgatacgcac gatttcaact gggtgctcga tggcaccgcg cgcaactacg tctacgtcga
    13501 cgatccgcgc gtgtatccat tcgacgagcc gggcttcatc gctgcgctgc gcgcgcgccg
    13561 tgtcgtcgcg acgaccggcc cgtggctcga tgtcgaggct gcggtgaaga aagacgccac
    13621 gccgacggtc gggccgggtg ggtggctcgt cgcgaacgac ggcgcgatct ggctcgacgt
    13681 gacgctgcag caggcgcgct gggtgaagac cgagcgcatc cgcatcacga tcggctcacc
    13741 cgaaggcccc aagctcgtgc aaacgatcga cgtgccgccg aacgtgcgcg agttccgctg
    13801 ggcgggccgc atcgacgtcg gcgcgcagga tacgtgggtt ggcgtcaccg ccgatggcga
    13861 cactgcgatg ccgctcgaat tcaccggcac gtaccagcgc gataaatggc agcacgcagg
    13921 cgtcacgccg atggcgctga tttccccgat tctcgtcgat gccgatcatg acgggcgttg
    13981 gaagcgtggg gacgctgacg taccgctggg tgtgcgatag gcgccgcacg agccgttgcg
    14041 ataccattgc tgcggatgga agcgttcgtt cagggctgga gcctcacgat cgcgctctgc
    14101 ggtttcatcg tgctggtgtt cgttgccggt tggttgcacg tccaccgcga gctggcctgg
    14161 gtcaaagcgc tcaccgatca cctcggtgac gagctcgtca gcgccgcgcc cctgaagccg
    14221 cgcagcctcg agctccaagc gctgcgcgac gaagcgcacg cgatcctcac cgccaaagac
    14281 ccagcctggg cgcagaagca ggtgcgcagc tggcaaatgc gcgcgcaacg gctcgagccc
    14341 gtgctcgcgt tctgggtcga tctgctccgc cagctcggtc tgctcggcac cgtgctcggc
    14401 attggcctct cgctcatgta cacgggcagc gatctgacaa agctgctcgg tccgctcgcg
    14461 ctcaaggtgt ggacgacggt cgccggtctc gcgtgtagca tcgcgctgtc cgcgagcttc
    14521 tcgatgaagc tgaccgcgtg ggtcgacgct gccgagaaga acctcgaggc gtgggatgcg
    14581 cgccgccacg ccggcaccgt gaaagcagag gacatgaagt gagacgcgcg cgtcgtcacg
    14641 tgcgcgtgcc gccggacctc acgtcgctgt tcgacgtgct gttcatcgtg atcttcgcgg
    14701 cgctgatccg cgctgcggcc gtcgagaaag cggccgcgca agcggcgacg ccgaagccgg
    14761 tcgcgccggc gccaccggtc aagctcgcga acgcgcagct gcacgagaag gcgctggccg
    14821 atctcggcaa acagctcgcg gcgcggccgt acgtggtcgt gcgcgtgagc gagcgcgcga
    14881 tcaaagcgct cgagactggc gacaatgtgc agaagctcga cactccactg ctcgagcaca
    14941 gc
//
LOCUS       DASUFX010000336        20572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1858066, whole genome shotgun sequence.
ACCESSION   DASUFX010000336 DASUFX010000000
VERSION     DASUFX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858066"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="VFV99_13465"
     CDS             complement(<1..1868)
                     /locus_tag="VFV99_13465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060368.1"
                     /translation="MLVNVGDVIAGRFEVERHVGAGGMGVVYRALDRDTSGAAALKVV
                     RGTDRATNERFEREVTVLAELRHPNIVGYVTHGKTNDGALYLAMEWLEGEDLSTRLSV
                     GTLSAAETVAVARQAAAALALAHQRGIIHRDIKPSNLMLVDNQPDRVKLLDFGIARLA
                     DIAPLTQTGMMIGTPLYMAPEQASGKSDIDVRADVFALGAVMFHCLTGRCPFPARTVA
                     ELVARLAAPTEAPRVRTFAPGTPLALDELVARMLAKDPAKRPRDAAAVHDALGPDVLA
                     QQSMATAETGLVETPRPDVHDDAGARGQKALAEGRWADAKAAFTEALAARDSGMIRLG
                     LAEALQWLGDCRGCIEQQEAAYALLRRTNDPMAALAAIQLGISQRKMMGNDTASRGWL
                     ARAARLLGNDVGPLHGYLWVANALDLDDPARAVELGGRALEAARKHNDRDLELIALAV
                     QGYAMVVNGDLDGGMALIDESMAGIVGGEYERLGTIVPTTCMMVNACHRMADVRRMVE
                     WCRGAEQFIDKYGAPFMFADCRAHYGSALMLTGRWDDAERELAKAMQAAPVETDYHAI
                     ARSQLADLRVRQGRLDEAAALLEELFDRACVRPIAAKIKFAAGRFTLPAGDADAAAP"
     gene            complement(1906..4203)
                     /gene="rpoD"
                     /locus_tag="VFV99_13470"
     CDS             complement(1906..4203)
                     /gene="rpoD"
                     /locus_tag="VFV99_13470"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX5060369.1"
                     /translation="MARTPAKKISKPAPKAPPAKDKGKAAPAKKDDKKEAKDAKDEKS
                     KKAVAPVAAPKAKPAAAPKAAKAPAKGGKVSAPRDEDGDGFDGDDDEDDDFAPPAAKA
                     GKKGAAAAGPGKSKGPRPEDLVDMDDDAVPGATDEFDDDDEEAGDRGPRRSSSADADA
                     AALEAETRATKKGRGAGNANAVTSAKDKLIELGKQKGFVTYDEVNDAMPEDVVSTDQI
                     DSWLSALGDHGIEVVDGTGPRRPDIVDPAPKGLDIEKEKEEGEVDEDEEYAYSRTSDP
                     VRMYLRKMGSVSLLTREGEVEIAKRIEDGERKMLQAVLNSSVAVEELIAIGDRLRHGK
                     IRVKDVVKDIDEDEAEFNEQFYVDRVCKIIDKVKRLQRDTEKLEEKLDEKGSTEGKKK
                     KVKEATKANRAQMFEELSDLRLNKPTVDRIVAQLKNIIVKLDKAEAEVKECERRAGMS
                     GSEIRKTLREMRANPTRARAVGKKTMLTVADFEEMEEQIKTAMRKVGGIELEARATAP
                     DLRSTFRDLHEGERMADRAKSELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMK
                     AVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRTTRYLVQ
                     ELGREPTPEEIAERMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSIIS
                     PSESVISVNLADQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIR
                     QIEAKALRKLRHPSRSKRLKAFVEN"
     gene            complement(4206..6200)
                     /gene="dnaG"
                     /locus_tag="VFV99_13475"
     CDS             complement(4206..6200)
                     /gene="dnaG"
                     /locus_tag="VFV99_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5060370.1"
                     /translation="MIADEIINEIRSRADIVAVIGQHVQLKKAGRNWKGLCPFHGEKT
                     PSFNVSADKGFYHCFGCQKHGDVFTFVMELEGKSFVEAAEQLGARFGVAVPKVDESPE
                     LRRARGEKVAMLDINKHATQFFREVLASTDSGKRGEPGRAYLAKRGVGAEISEKFQLG
                     YAPPDWHALADYLKAKGCDMELATKLGLVARQPRAGGYYDRYRDRLVCPVIKVGNEVA
                     GFSARVIGKPPVAPDGSEPPKYINSPESPVYKKSQLLFGLAQAREGMQKNGRAVLVEG
                     NFDVISLHQAGFSDVVAPLGTALTIEQVLTLKRLAERIVLLYDGDKAGYKATMHALQL
                     CVEADVEVLVAHRPGNAKSGGGGVLAGGVDPDSLVASGGAEQLREAIDRAQGGIEFFC
                     FEVWGKARANADAKTRALEDAARLATHIENPIKRDLVIDTLAKALEVDTSVVRTAVGR
                     ASGSRSGGARTDERGRAASHPNAPGGHPNAPAGYGGAPDARDGRGPGSDETQRANPAK
                     TLGPPPALEIELISLLADHPSLIASPEADKAFWLLTDERVRSMYSAARAGQSFLELAP
                     VQLPPPSAKQVLSGTYSESKDPRAQLLALIASLEQKQTMLANEGLLKSLVDAKRGGSD
                     PELVRLRAQLAVAQAKRDPELVEQLKAEIEKLASNRKQAE"
     gene            complement(6273..6482)
                     /locus_tag="VFV99_13480"
     CDS             complement(6273..6482)
                     /locus_tag="VFV99_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060371.1"
                     /translation="MAEHPGTARKKHRHDLGEEDHGPKLAAGTTRAIQTLLGLWIALT
                     LVFVIYALVDASRPKAEIVETTPAK"
     gene            complement(6575..7975)
                     /locus_tag="VFV99_13485"
     CDS             complement(6575..7975)
                     /locus_tag="VFV99_13485"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060372.1"
                     /translation="MVRLGEHLAAARLLDQEQIDRALRAQVLWGGRLGTNLIELGLLD
                     LDRISRALGRQHRVPAALSHHFEKADRELQERLTPELAKQWSVVPLRHVGPKKQIAIA
                     VLDPLPADALAELADAFLCGRENILVTVAAEMRVRYHLERVYGIKRPARFLRTKGPTI
                     TPFPQFENVPVPVDSDVEVAVPIAVDDSAHPTGRASADDLTVPPEIERATSGVPQGIA
                     HATTAVMEAPPPASVDDSDALMQLIDDAVAQAMAVEPPEPPVAGRDRRTYVRTLADAP
                     PGPQTPAPASVVTGAALARMSIKRMAVTEAKPAAQTFVEGVRAIKRAGNRDRVAELVI
                     ETLQRFVPACEAAVLLVIRGEVATSWGHFSRTSATSPEIAVPLDQPGLLPGVIANNAV
                     ARSGFADLTAIDEALMRSLGEPLGDLVVLPIAIADQVMALIAASTEAGAPIGGIESVA
                     FAAGAAFARLIRDASR"
     gene            complement(7991..9055)
                     /locus_tag="VFV99_13490"
     CDS             complement(7991..9055)
                     /locus_tag="VFV99_13490"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5060373.1"
                     /translation="MDIRNIREILGDKASLLEHQSKTIPASSLTLPSPRYIDEIFSTS
                     DRTPRVLGAMQQMFDHGRLAGTGYLSILPVDQGIEHSAGASFAPNPQMFDPEHIIRLA
                     IEGGCSAVASTFGVLGMMARKYAHRIPFIVKINHNELLSYPNTHDQTLFGNVKQCFDM
                     GAQGVGATVYWGAPESRRQLHEISEAFAHAHELGLFTVLWCYVRNSAFKTSQGDHSLS
                     ADLTGQANHLGVTIQADIIKQKLPELNGGYNDPALKGFGKTHKKVYSELTSDNPIDLT
                     RYQVANCYMGRIGLINSGGASTGESDLREAVTTAVINKRAGGMGLIAGRKAFQRPFKE
                     GVELLQAIQDVYLCKDVTVA"
     gene            9230..10084
                     /locus_tag="VFV99_13495"
     CDS             9230..10084
                     /locus_tag="VFV99_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060374.1"
                     /translation="MDIGFNLPTVTLKANHEVADDQWEEIGDADLSCLNTASADMATS
                     VSVTMNAVVKDFQSGNAVPGAKVTAFKDVDYMNPFQAEVTADTNAELMVTIPTGVKRF
                     GYRMTGSTSLPTFLLNQTVKPDVAVQPEGTCTPAPCREKIQSVSNATAATLPALIGQT
                     RTVGTGVVAGALRDCQRREISGFIATISSVSAMHETIMGADSYYFDSAVGLPVHHNRQ
                     AFASGDGLFMIIEVPSTNPTGYVQMWGFPTEDDKAMGQAGLKLIAELKVPVLADTVIT
                     GSYEPLRQ"
     gene            10105..10869
                     /locus_tag="VFV99_13500"
     CDS             10105..10869
                     /locus_tag="VFV99_13500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5060375.1"
                     /translation="MSAPAASPEVTSSPDLRAGRHALYTYVAVAVLVAGLVRINITLP
                     AIGHLGSALVSVLFLYAPMIVLGRRKEDLIDYGFKAEPIKRGLLTAAAAIAIVFPIFV
                     VGYFVFYEVACHSKLLAHLVPHGMCAQYGGMRALHVPTPTWSLGEFCLVQLLVVALPE
                     ELFFRGFLLGLLEKRFPPKRRILGGGVGLALVLSSLAFAIIHIPKDGDPRVLATFFPG
                     LLFGWMRSATGSILASTVTHGCSNILIQLLVLAVKR"
     gene            10866..11516
                     /locus_tag="VFV99_13505"
     CDS             10866..11516
                     /locus_tag="VFV99_13505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5060376.1"
                     /translation="MTVAAFFDMDNTLLTVDTGVSYTRWLYRRGELSRRMLAKALYWS
                     TLYKLAVLDMETVFTKLCLGLEGDSEADMIAKCDIWYREHIAPFVAPAARVALEHHRQ
                     LGHAVVLATGSTVYAARPVAAGIGIDHVLATELEVDGDTFTGRPRALCFGHHKVKLAE
                     QWAASHGIDLARSYFYSDSFNDRPMLERVGHAIAINPDARLSRLAKQRGWPMMRWS"
     gene            11516..11998
                     /locus_tag="VFV99_13510"
     CDS             11516..11998
                     /locus_tag="VFV99_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060377.1"
                     /translation="MRTLLTLVIVALICGVAGADVYKSKAGRVSIEVPKKWSVMAQDD
                     LVRGASPNNEVAVVMWIVDSAEVKDALKKLEGELYSAIQGLRWIDKTKKLKVNKIAGT
                     WVEGVGVSSRSTQLDVIAFVAATPTKKGVIVLAVVDHDKYEANRSAIQSIFGTLKTTK
                     "
     gene            12074..12940
                     /gene="nadC"
                     /locus_tag="VFV99_13515"
     CDS             12074..12940
                     /gene="nadC"
                     /locus_tag="VFV99_13515"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5060378.1"
                     /translation="MGDFALRGLAAIPAVRRLIELALDEDLGRGDVTSQVTLGNEGRR
                     VVADMNAREPIVAFGVDVAAAVFSLVDPRIEVEVLAPDGTRVDKGGLIVTVTGPAHNV
                     LAAERTALNFIQRLSGVATLSKRYADHVAGTKAKVVDTRKTTPGYRVLEKAAVLAGGC
                     ANHRFDLGSGILIKDNHIAAAGSVRAAVEAAKARAPHPLRVEVEVTNLGELDEALAAG
                     AEIVLLDNMTPSQVEVAAARAHGRGVLVEVSGGITLATIADYARAGADLISVGALTHS
                     APAVDIGLDVRT"
     gene            12937..13704
                     /locus_tag="VFV99_13520"
     CDS             12937..13704
                     /locus_tag="VFV99_13520"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5060379.1"
                     /translation="MTFEALHLKARWLGCQRIVLDSCGSTNDEAARLARAGAKHGTIV
                     IAERQKSGRGRDGRTWESPPGGLYMSAVVRPPIPVADVPPMTLAIGIGLCEAARTTGA
                     AAVLKWPNDLLVRHRKLAGVLVEAQSQANRLEAVIIGIGVNVGGELPEPVAESGITLA
                     EAAGTTIDRDAFIETLLAHVERWVDQYVSVGIEPIIPAWQARMAVGLAARATVDGASL
                     TGCVAGLDADGALLLRDEDGNVHRVRSGDVEVLRAAC"
     gene            13753..14382
                     /locus_tag="VFV99_13525"
     CDS             13753..14382
                     /locus_tag="VFV99_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060380.1"
                     /translation="MRNLLVFGGLAVVALCVGLWLLLHKGDDASAETGTTQGSQAVVA
                     PPHTSGTPSVTSAPPGEHPALPTVGSGENPRDYVVGDIRVRDHRAGDNKPLDIPPNVH
                     PPEGPAIPSELTHAVAQKVKAVMIDCVASLPQDARGEKPRLEGQIEISIKDHKVRIEK
                     STMQLRNVSGESVEPTRQCIESKSVGIENSAPDQSDLASYPINISFAIP"
     gene            14540..15643
                     /locus_tag="VFV99_13530"
     CDS             14540..15643
                     /locus_tag="VFV99_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060381.1"
                     /translation="MSRTSVRAVRLLGMVTLLVAITSTLGCEQLDGRNRNKKGNRYFR
                     EMKFIDAVAEYEKALKTVDDPIIHYNVALAYSKIFKPGMETDQELLLGEKGSFVCDQV
                     PQVKFVSKQVCVKPGDRRYDACDAKNVCASSYTCKQTELCAIGNNAIADMTAQHFGVW
                     IKVHPKDAETRGLMTQVWMDSGQYKKALDYWQSLLSDKPNDTEIMGALAGINLKAGDW
                     RKSIEWYTKVAELSKEESAKVAAYQFIGNVAWSKLNSKTLTTADSVELADRGIGALQK
                     AAEIQPKNPKLFGLMGSIFNFRSLAMGASWAAGLDRATAQDLQIVSRVLSDEAKKAQG
                     LPVTPTPAPAPAPSPAPSGSAAPATPSPAKTGG"
     gene            15670..16416
                     /locus_tag="VFV99_13535"
     CDS             15670..16416
                     /locus_tag="VFV99_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060382.1"
                     /translation="MFANYAAAKNRKLPKWAGPLLVGAVIFHIGLFVTMWIKSIWDIE
                     LLERPKTTIDLAVAPPPPPPPPPPPGGAKPQEVRIEPKKIKVKDIVQPVKIEKQVAQV
                     QDTGPAGVEGGVEGGVEGGVVGGDLNGVMGAPPPPPPPPPPAPPQNVPPTLLEGSRIA
                     GEKMISPDDVTKTEIQRSGKDRIIGSFKLCLTVTGAISNVNMLKSTGFPAYDGKIQNK
                     MRSEWRYKPYMVNGKAVPVCTAVTFIYSQK"
     gene            16444..17130
                     /locus_tag="VFV99_13540"
     CDS             16444..17130
                     /locus_tag="VFV99_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5060383.1"
                     /translation="MGLDLMELWEHMSIVVKIITVLMVLMSVFMFYIIAERLLTFRAA
                     SDQSIRYVQALGEYLRQHKVNEALTAARGANKSPVAKVMESGLAALLQGREALTTQGP
                     ADVGDFDLVDSVNRALERVKERETSNLRRGLAFLGTTASATPFIGLLGTVVGILGTFQ
                     MLKGGTVTINEIGPKISEALVSTALGLGVAIIAAMSFNFFTSRVEQFVIDMNDVSSEF
                     IDYVLREGRS"
     gene            17136..17648
                     /locus_tag="VFV99_13545"
     CDS             17136..17648
                     /locus_tag="VFV99_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5060384.1"
                     /translation="MAGAPQKNSGNAGHPHRRWRLSGKKHHIDSVRNDINVTPLVDVM
                     LVLLIIFMLMTLIMGRGHDVNLPKAANYSQEKDKMQPVVTIDLEGNLFVEKNKLGPVN
                     ESTLKEMANQIQAAWAAPKNPDGVGRIYIKADSKVGYGKVHPVLMYLNRELQLQQIDL
                     AIAKDSKEGD"
     gene            17651..18127
                     /locus_tag="VFV99_13550"
     CDS             17651..18127
                     /locus_tag="VFV99_13550"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5060385.1"
                     /translation="MGMSTGSGDKRAVQSEINVTPLIDVLLVLLIIFLVVMPIMMKME
                     TLQVPRKLDDQNEMPDPNASQLTVKVKNDLSIAFNDGSSENDMPIKAVDIASTLRPKL
                     EAMQANQEKVVFVEFEEGVPWSDVVGTMDTIRSLAADVNHDDIKVALKIKEDEQKQ"
     gene            18301..18705
                     /locus_tag="VFV99_13555"
     CDS             18301..18705
                     /locus_tag="VFV99_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5060386.1"
                     /translation="MAFTQRGSAEINVTPLIDVLLVLLVIFLIVMPTMVKFEEVHTPA
                     PPEQSGEYVQSLVVRVHADTSVVLVDEGREIPLDAGGQLAGGLRSHLPARTQVVFLEL
                     EDDVQWGHVVSTIDTIHGVAPMTPVALKVADE"
     gene            19009..20109
                     /locus_tag="VFV99_13560"
     CDS             19009..20109
                     /locus_tag="VFV99_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5060387.1"
                     /translation="MIRQVRLKEKYQDATGVLTARGNPVFAFGTHPQITGPDDAHPVT
                     SATAVGNKLRIIMDELLVGNNLQQIECRQPVNTDGAFDSVPIGATPDDVARCSVASDV
                     LPKSCPASNPHSMCICQIATGCGVTGEIKMNMPVGVKDVNQDGAADGMRMMPNSVGLK
                     CGSIDVPIDLNNSYWNPSGDQNVPAMGGFDALGPAIVLQPDHPLGMASIPQFLPTNTT
                     CSLVFDPAVVDKSGIKVCAPPDGDITKNCTPGNVDAFTFRVEPLAIDNALSNVHDGDT
                     GVSRTDGVQLYMTAPVSAASISAITVTEGSTSFTAFTATQSAPDQIKLTWTGATGLAA
                     NTMYTITVGTGLTDVYNQAAPMPSTISFMTGS"
     gene            20118..>20572
                     /locus_tag="VFV99_13565"
     CDS             20118..>20572
                     /locus_tag="VFV99_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5060388.1"
                     /translation="MKTRKLLLAATLGSTSLFVANLINPDLAAAQSSTAGAIQGVVTD
                     GKSGEKLAGVTVIVTSPALAQTQTAITDENGAYQVGALPPGEYLVTFYYADITVERSG
                     IRVGIDKTTPVYQKLNMAAAGGETIKISDTAPTIDPTSTTQGITIDKNYL"
BASE COUNT         3462 a         6720 c         6672 g         3718 t
ORIGIN      
        1 ggggctgccg cgtcggcatc cccggcgggg agggtgaagc gaccggcggc gaatttgatc
       61 ttcgcggcga tcggtcgcac gcacgcgcga tcgaatagct cctccagcag cgcggctgct
      121 tcgtcgagcc ggccttggcg cacgcgcagg tcggcgagct gcgagcgcgc gatcgcgtgg
      181 tagtcggtct cgaccggcgc tgcttgcatc gccttcgcca gctcgcgctc ggcatcgtcc
      241 cagcgccccg tcaacatcag tgcgctgccg tagtgagcgc ggcaatctgc gaacatgaat
      301 ggcgcgccgt acttgtcgat gaactgctcg gcaccgcggc accattcgac catgcgccgg
      361 acatcggcca tgcgatggca cgcgttcacc atcatgcacg tcgtcggcac gatcgtgccg
      421 agtcgttcgt attcaccgcc gacgatgccg gccatcgact cgtcgatcag cgccatgccg
      481 ccgtcgagat cgccgttcac caccatcgcg tagccttgca cggcgagcgc gatgagctcg
      541 aggtcgcgat cgttatgctt gcgcgctgcc tcgagcgctc gcccgccgag ctcgaccgca
      601 cgtgccggat cgtcgaggtc gagcgcgttc gcgacccaca aatacccgtg cagcgggccg
      661 acgtcgttgc cgagcagtcg cgcggcgcgt gcgagccagc cgcgactcgc ggtgtcgttg
      721 cccatcatct tgcgctgact gatgccgagc tggatcgccg caagggcggc catcggatcg
      781 ttcgtgcgcc gcagtagcgc gtacgcggct tcctgctgct cgatgcagcc gcggcaatcg
      841 ccgagccact gcagcgcttc ggcaagcccg agccggatca tgccggagtc gcgcgcggcg
      901 agcgcttcgg tgaacgcggc cttcgcgtca gcccagcgtc cttccgcgag cgccttctgg
      961 ccgcgtgcgc ctgcatcgtc gtggacgtcc gggcgcggcg tctcgacgag gccggtctcg
     1021 gcggtggcca tcgactgctg tgcgagcacg tcggggccga gtgcgtcgtg caccgcggcg
     1081 gcatcgcgtg gccgcttcgc cggatccttc gcgagcatgc gcgcgacgag ctcgtcgagc
     1141 gctagcggcg taccgggtgc gaacgtgcgg acgcgcggcg cttcggtcgg cgcggcaagg
     1201 cgtgcgacga gctcggcgac cgtgcgggcc ggaaatgggc agcgtccggt caggcagtgg
     1261 aacatcacgg caccgagcgc gaacacatcg gcgcgcacgt cgatgtcgct cttcccactc
     1321 gcctgctcgg gggccatgta cagcggcgtg ccgatcatca tgccggtctg cgtgagcggt
     1381 gcgatgtcgg cgagccgcgc gatgccgaag tcgagcagct tgacgcggtc gggctgattg
     1441 tcgacgagca tcaagttcga cggcttgatg tcgcggtgga tgatgccgcg ctgatgcgcg
     1501 agcgcgagtg cagccgccgc ttggcgggcg accgcgacgg tctctgccgc cgacagcgtg
     1561 ccgaccgaga gccgcgtcga gagatcttcg ccctcgagcc attccatggc gaggtagagc
     1621 gcaccgtcgt ttgtcttgcc gtgcgtgacg tagccgacga tgttcgggtg ccgcagctcg
     1681 gcgagcacgg tcacttcgcg ctcgaagcgc tcgttcgttg cgcgatcagt tccacgcacg
     1741 accttgagcg cagctgcgcc agaggtgtcg cgatcgagcg cgcgatagac gacgcccata
     1801 ccgccggcgc cgacgtgcct ctcgacttcg aatcggcccg cgataacgtc cccgacgttt
     1861 accagcacat gactagcaaa ccacggggag cgctactgcg tcagctcagt tctcgacaaa
     1921 cgccttgagg cgcttgctac gcgacggatg acgcagcttg cgcagcgcct tcgcttcgat
     1981 ctggcggatg cgctcgcggg tgacttcgaa gtcctgaccg acttcctcga gcgtgtggtc
     2041 gctcttctca ccgatgccga agcgcatgcg gaggaccttc tcctcgcgcg gtgtgagcgt
     2101 cgcgagcacc ttgcgggtct gatccgcgag gttgacgctg atgacgctct cgctcggcga
     2161 gatgatgctc ttgtcctcga tgaagtcgcc gaggtgggag tcttcctcct caccgatcgg
     2221 cgtctcgagc gagatcggct ccttcgcgat cttgaggacc ttgcggacct tgtcgagcgg
     2281 cagctccatg cgctcggcga tctcttccgg ggtcggctcg cggccgagct cctggacgag
     2341 gtagcgcgtc gtgcggacga gcttgttgat cgtctcgatc atgtggaccg ggatgcggat
     2401 cgtgcgcgcc tggtcggcga tcgcacgggt gatcgcctgg cggatccacc acgtcgcgta
     2461 cgtcgagaac ttgtagccgc gcttgtactc gaacttgtcg acggccttca tcaggccgat
     2521 gttgccttcc tggatcaggt cgaggaactg caggccgcgg ttcgtgtact tcttcgcgat
     2581 cgacacgacg aggcgaaggt tcgcttcgac gagctcgctc ttcgcgcgat ccgccatccg
     2641 ctcgccctcg tggaggtcgc ggaacgtcga gcgcaaatcg ggcgccgtgg cgcgggcctc
     2701 gagctcgatg ccgccgacct tgcgcatcgc cgtcttgatc tgctcttcca tctcctcgaa
     2761 gtcggcgacg gtgagcatcg tcttcttgcc gacggcgcgg gcgcgcgtcg ggttcgcgcg
     2821 catctcgcgc agcgtcttgc gaatctcgct gccgctcatg ccggcgcggc gctcgcactc
     2881 tttgacttcg gcctcggcct tgtcgagctt gacgatgatg ttcttgagct gcgcgacgat
     2941 gcgatcgacc gtcggcttgt tgaggcgcag atcgctgagc tcttcgaaca tctgagcgcg
     3001 attggccttg gtcgcttcct tgaccttctt cttcttgccc tcggtcgagc ccttctcgtc
     3061 gagcttctcc tcaagcttct cggtgtcgcg ctggaggcgc ttgaccttgt cgatgatctt
     3121 gcagacgcgg tcgacgtaga actgctcgtt gaactcggcc tcgtcctcgt cgatgtcctt
     3181 gacgacatcc ttgacgcgga tcttgccgtg gcgcaggcga tcgccgatcg cgatcagctc
     3241 ttcgacggcg accgagctgt tgagcacggc ctggagcatc ttgcgctcgc cgtcctcgat
     3301 gcgcttcgcg atctcgactt cgccttcacg cgtgagcagc gagaccgagc ccatcttgcg
     3361 caggtacatg cggacgggat cgctggtgcg cgagtacgcg tactcctcgt cctcgtcgac
     3421 ttcgccttct tctttttctt tttcgatgtc gaggcccttc ggggccggat cgacgatgtc
     3481 gggacggcgc ggacccgtgc cgtcgacgac ctcgatgccg tgatcaccga gcgccgagag
     3541 ccagctgtcg atctgatcgg tcgacaccac gtcttcgggc atcgcgtcat tgacctcgtc
     3601 gtacgtgacg aagcccttct gcttgccgag ctcgatcagc ttgtccttcg cgctcgtaac
     3661 cgcgttggcg ttgccggcac cacggccctt cttggtggcg cgcgtctcgg cttcgagcgc
     3721 agcggcatcg gcatctgccg acgagctgcg acgcgggccg cgatcgcccg cctcctcgtc
     3781 gtcgtcgtcg aactcatcgg tcgcgccggg gaccgcgtcg tcgtccatgt cgacgaggtc
     3841 ttcggggcgc ggacccttgc tcttgcccgg gcccgccgca gcagcgccct tcttgccggc
     3901 cttcgcagcc ggtggcgcga agtcgtcgtc ctcgtcgtcg tcgccgtcga agccatcgcc
     3961 gtcttcatcg cgcggcgcgg agaccttgcc gcccttcgcg ggagccttcg ccgccttcgg
     4021 cgcggccgcc ggcttcgcct ttggcgcagc aaccggcgcg accgccttct tggacttctc
     4081 gtccttggcg tccttcgcct ctttcttgtc gtccttcttg gcaggcgctg ccttcccctt
     4141 gtccttggcg ggcggagcct tcggagcggg cttactaatc ttcttcgctg gggtacgtgc
     4201 catcgttact ctgcctgctt tcggttggac gcgagcttct cgatctcagc cttgagttgt
     4261 tcgaccaact cagggtctcg tttagcttga gctacggcta gctgcgctct gagacgcact
     4321 agctctggat cactgccacc acgcttcgca tcgacgagag acttgagcag tccctcgttc
     4381 gcgagcatcg tctgcttctg ttccagcgaa gcgatcaacg cgagcagctg tgctcgcgga
     4441 tctttgcttt cggaatacgt tccggacagc acttgcttgg cgctcggcgg gggaagttgc
     4501 acgggggcaa gctccaggaa ggactgtccc gcccgggccg cggaatacat ggaacgaacc
     4561 cgttcgtccg tcaagagcca aaaagccttg tctgcctccg ggcttgcgat cagtgaaggg
     4621 tggtctgcga gtagggaaat cagctcgatc tcgagggcgg gcggcgggcc gagagttttc
     4681 gcgggattcg cgcgttgcgt ctcgtcggaa cccggacccc ggccgtctcg cgcatcaggt
     4741 gcgccgccat agcccgccgg ggcattcggg tggccgcccg gggcgttcgg gtgacttgcc
     4801 gcacgccctc gctcgtcggt gcgggcgccg cccgaccggg aaccggacgc gcggccgacg
     4861 gccgtccgca ccacggacgt gtcgacctcg agcgccttcg cgagcgtgtc gatcacgagg
     4921 tcgcgcttga tcggattctc gatgtgcgtc gcgagccgcg ctgcatcctc gagcgcacgg
     4981 gtcttggcgt cggcgttcgc gcgcgccttg ccccagacct cgaagcagaa gaactcgata
     5041 ccgccttgcg cgcgatcgat cgcttcgcgg agctgctcgg cgccaccgga cgcgacgagg
     5101 ctgtcgggat cgacgccgcc cgcgagcaca ccgccgcctc ccgacttcgc attaccgggc
     5161 cgatgcgcga cgagcacctc gacgtccgcc tccacgcaca gctgcaacgc gtgcatcgtg
     5221 gctttgtagc ccgccttgtc gccgtcgtag agcagcacga ttcgctcggc gagacgcttg
     5281 agcgtcagca cctgctcgat cgtgagcgcc gtccccagcg gcgcgacgac atcggagaat
     5341 ccagcctggt ggagcgaaat gacgtcgaag tttccttcaa ccagcaccgc gcgcccattt
     5401 ttttgcatgc cttcgcgcgc ctgcgcgagc ccgaatagca gctggctctt cttgtagacc
     5461 gggctttcgg ggctgttgat gtatttgggc ggctcgctgc cgtcgggggc gaccggtggc
     5521 ttgccgatca cgcgcgccga gaatccggcg acttcgttgc cgaccttgat caccggacac
     5581 acgaggcgat cgcgatagcg atcgtagtaa ccgccggcgc gcggctgccg tgcgacgagc
     5641 ccgagcttcg tcgcgagctc catgtcgcag cccttggcct tcaggtagtc ggcgagcgcg
     5701 tgccaatccg gcggcgcata gccgagctgg aacttctcgc tgatctcggc gccgacgccg
     5761 cgcttcgcga ggtacgcccg ccccggctcg ccgcgcttgc cggagtctgt cgacgcgagg
     5821 acctcgcgga agaactgtgt cgcgtgcttg ttgatgtcga gcatcgcgac cttctcgccg
     5881 cgtgcgcgcc gcaattccgg cgactcgtcg accttgggca ccgcgacgcc gaaccgcgca
     5941 ccgagttgct cggccgcctc gacgaagctc ttgccctcga gctccatcac gaacgtgaac
     6001 acgtcgccgt gcttctggca gccgaagcag tgatagaagc ccttgtcagc ggagacgttg
     6061 aacgacggcg tcttctcgcc gtggaatgga cacagaccct tccagttgcg acccgctttc
     6121 ttgagctgaa cgtgctggcc gatcaccgcg acgatgtctg cgcgactgcg gatctcgttg
     6181 atgatctcgt cggcaatcat gtccagcagc gctcccccga gcgcagtctc cgcaccatga
     6241 ccgatccgtc cttcgttgac caggtcgccg gactacttcg ccggcgtggt ctccacgatc
     6301 tcggccttcg ggcgcgatgc gtcgacgagg gcgtagatca cgaagaccag ggtcagggcg
     6361 atccacagcc cgagcagggt ctggatcgcg cgcgtggtgc cggccgcgag cttgggaccg
     6421 tgatcctctt caccgaggtc atggcggtgc ttcttgcgag ccgtacccgg gtgttcagcc
     6481 ataacgaccg gacgttgccg tacgtgtctg acatcgtcaa gccagccccg cgtcacatcg
     6541 ggtggaccgc tttcaccgac ctgatcgcgg ccggttagcg gctggcatcg cggatcagcc
     6601 gcgcgaacgc agcgccggcg gcgaacgcga ccgactcgat gccgccgatc ggcgcaccgg
     6661 cctcggtact cgcggcgatc agcgccatca cctggtcggc gatcgcgatc ggcaaaacga
     6721 cgagatcgcc gagcggctcg ccgagcgagc gcatcaacgc ttcgtcgatc gcagtcaggt
     6781 cggcaaaccc gctgcgcgcg acggcgttgt tcgcgatcac gcccggcaga agacccggct
     6841 ggtcgagcgg cacggcgatc tccggcgagg tcgcgctcgt gcgcgagaag tggccccaac
     6901 tcgtcgccac ctcgccgcgg atcacgagca gcaccgctgc ttcgcacgcc ggcacgaagc
     6961 gctgcagcgt ctcgatgacg agctcggcga cgcgatcgcg gttgcccgcg cgcttgatcg
     7021 cacgcactcc ctcgacgaac gtctgcgcgg cgggcttcgc ctcggtgacc gccatgcgct
     7081 tgatcgacat gcgagcgagc gcggcgcccg tcacgaccga cgcgggtgcg ggcgtctgcg
     7141 gcccaggcgg cgcgtcggcg agcgtgcgca cgtaggttcg ccgatcgcgg ccggcaacgg
     7201 gcggctccgg tggctcgacg gccatcgcct gagcgaccgc gtcgtcgatc agctgcatca
     7261 gcgcgtcgga gtcgtcgacg ctcgcgggtg gcggggcttc catcaccgcg gtcgtcgcgt
     7321 gcgcgatgcc ctgcggcaca ccactcgtcg cgcgctcgat ctccggcggc accgtgaggt
     7381 cgtcggcgct cgcgcggccc gtcgggtgcg cgctgtcgtc tacagcgatc gggaccgcga
     7441 cctcgacgtc gctgtcgacc ggaaccggca cgttctcgaa ctgcgggaac ggcgtgatcg
     7501 tcgggccctt cgtgcgcagg aagcgggcag ggcgcttgat tccgtagacg cgctcgaggt
     7561 ggtagcgaac gcgcatctcg gcggcgacgg tgacgaggat gttctcgcgc ccgcacagaa
     7621 acgcgtccgc gagctcggcg agcgcgtcag ccggcaatgg atcgagaacg gcgatcgcga
     7681 tctgcttctt cgggccgacg tggcgcagcg gcaccaccga ccactgcttt gcgagctccg
     7741 gcgtcagtcg ctcctggagc tcgcgatcgg ctttctcgaa gtgatgcgag agcgcagccg
     7801 gcacgcgatg ctgacggccg agcgcacgcg agatgcgatc gagatcgagg aggcctagct
     7861 cgatcaggtt cgtaccgagc cggccacccc acaacacctg cgcccgcagc gcgcggtcga
     7921 tctgttcctg atcgagtagg cgtgccgcag cgaggtgctc gcccagtcgg accatggacc
     7981 tttgtactta ctacgcgacg gtgacgtctt tgcagagata gacgtcctga atcgcctgga
     8041 gcagctcgac gccttccttg aacggacgct ggaacgcctt gcggcccgcg atcaggccca
     8101 tgccaccggc gcgcttgttg atcaccgcgg tggtgaccgc ctcgcgcaga tcgctctcgc
     8161 ccgtcgacgc gccgcccgag ttgatgagac cgatgcggcc catgtagcag ttcgcgacct
     8221 ggtaacgcgt gaggtcgatc gggttgtcgc tcgtcagctc ggagtaaacc ttcttgtgcg
     8281 tcttgccgaa gcccttgagc gcgggatcgt tgtagccgcc gttcagctcg gggagcttct
     8341 gcttgatgat gtcggcctgg atcgtgacgc cgaggtggtt cgcttgaccg gtgaggtccg
     8401 ccgacaagct gtgatcacct tgcgacgtct tgaacgcgct gttgcggacg tagcaccaga
     8461 ggaccgtgaa caacccgagc tcgtgcgcat gtgcgaacgc ttctgagatc tcgtgcagct
     8521 ggcggcgcga ttccggcgcg ccccagtaca ccgtcgcgcc aacgccttgc gcgcccatgt
     8581 cgaagcactg cttcacgttg ccgaacagcg tctggtcgtg cgtgttcgga tagctcaaca
     8641 gctcgttgtg gttgatcttg acgatgaacg ggatccgatg cgcgtacttg cgcgccatca
     8701 tgccgagcac gccgaacgtc gacgcgacgg cgctgcagcc gccctcgatg gcgagccgga
     8761 tgatgtgctc gggatcgaac atctgcgggt tcggcgcgaa cgacgcgccg gccgagtgct
     8821 cgatgccctg gtcgaccgga aggatcgaca gataaccggt gcccgcgagt cggccgtgat
     8881 cgaacatctg ctgcatcgca ccgaggacgc gcggcgtgcg gtccgatgtg ctgaagatct
     8941 cgtcgatgta gcgcggcgac ggcagtgtga gcgatgacgc aggaatcgtc ttgctctggt
     9001 gctcgagcag cgaggccttg tcgccgagaa tctcccggat gttgcggatg tccatgcaca
     9061 gtgtttagcg ccacatcgga aatggttgca atcgggggcg gtcgcccgat acatacggac
     9121 accgggagtt agccaaatgc gcctacctat tcttttcacg cttccctcac tgttgctttt
     9181 tgcatcatgc ggcggcgacg acgggtctac gacgcccgac gcagccggca tggacatcgg
     9241 cttcaacctg ccgaccgtca ccctcaaggc gaaccacgag gttgccgacg atcagtggga
     9301 ggagatcggc gacgccgacc tcagctgcct gaacacggcg tcggccgaca tggcgacgtc
     9361 ggtgtcggtc acgatgaacg ccgtcgtcaa agacttccag agcggtaacg ccgttccggg
     9421 cgccaaagtc accgcgttca aggacgtgga ctacatgaac ccgttccagg cggaagtcac
     9481 cgccgacacg aacgccgagc tcatggtcac gatcccgacc ggcgtgaagc gcttcggcta
     9541 ccggatgacg ggctcgacgt cgctgccgac gttcctgctc aaccagacgg tgaagcccga
     9601 cgtcgcggtg cagcccgagg gcacctgcac gccggcgccg tgccgcgaga agatccagag
     9661 cgtgtcgaac gcgaccgccg cgacgctgcc cgcgctgatc ggccagacgc gtacggtcgg
     9721 caccggcgtc gtcgcgggcg cgctgcgcga ctgccagcgc cgcgagatct ccggcttcat
     9781 cgcgacgatc tcgtcggtgt cggcgatgca cgagacgatc atgggagcgg atagctacta
     9841 cttcgactca gcggttggct tgcccgtgca ccacaaccgc caggcgtttg cctcgggtga
     9901 cggcctgttc atgatcatcg aggtcccgtc gacgaacccg accggttacg tccagatgtg
     9961 gggcttcccg accgaggacg acaaggcgat gggccaggcc ggcctcaagc tgattgccga
    10021 gctgaaggtt ccggtgctcg cggacaccgt gatcaccggt tcgtacgagc cgctgcgcca
    10081 gtaatcgatt aacgtagcgc agcagtgtct gcgccggcgg cttctcccga ggtgacctcg
    10141 tcgcccgacc tgcgcgcggg gcgccacgcg ctctatacgt acgtcgccgt ggccgtcctg
    10201 gttgccggcc tcgtacggat caacatcacg ctgccggcga tcggtcatct cggcagcgcg
    10261 ctggtctcgg tgttgttcct gtacgcgccg atgatcgtgc tgggccggcg caaggaagac
    10321 ctgatcgact acggcttcaa agccgagccg atcaagcgcg gcctgctcac tgccgctgca
    10381 gcgatcgcga tcgtctttcc gatcttcgtc gtcggttact tcgtcttcta cgaggtcgcc
    10441 tgtcatagca agctcctcgc gcacctggtg ccgcacggca tgtgcgccca gtacggcggc
    10501 atgcgcgcgc tccacgtgcc gacgccgacg tggtcgctcg gcgagttctg tctcgtgcag
    10561 ctgctcgtcg tcgcactgcc cgaagagctg ttctttcgcg gcttcttgct cggtctgctc
    10621 gagaagcggt ttccgccgaa gcgccgcatc ctcggcggcg gcgttgggct tgcgctcgtg
    10681 ctgtcgtcgc tcgcgttcgc gatcattcac attcccaagg acggcgaccc gcgcgtgctc
    10741 gcgacgttct tcccgggcct gttgttcggc tggatgcgct cggcgaccgg gtcgattttg
    10801 gcctcgaccg tgacgcacgg ctgctcgaac attctgattc agctgctagt cctagcggtg
    10861 aagcgatgac cgtcgccgca ttcttcgaca tggacaacac gctgctcacc gtcgacaccg
    10921 gcgtgagcta cacgcgctgg ctctatcggc gcggcgagct gtcgcgccgg atgctcgcga
    10981 aggcgctcta ctggtcgacg ctgtacaagc tcgcggtgct cgacatggag acggtgttca
    11041 cgaagctgtg tctcgggctc gagggcgact ccgaggccga catgatcgcg aagtgcgaca
    11101 tctggtaccg cgagcacatc gcgccgttcg tcgcgcctgc tgcccgcgtc gcgctcgagc
    11161 atcaccgcca gctcggccac gcagtcgtgc tcgcgaccgg ctcgacggtg tacgcggcac
    11221 gcccggtcgc agccggcatc ggcatcgacc acgtgctcgc aaccgagctc gaagtcgacg
    11281 gcgacacgtt caccggtcgg ccgcgcgcgc tgtgctttgg tcatcacaag gtgaagctcg
    11341 ccgagcagtg ggcggcgtca cacggcatcg acctcgccag gagttacttc tactccgaca
    11401 gcttcaacga ccggccgatg ctcgagcggg tcggccacgc gatcgcgatc aaccccgacg
    11461 cccggctgtc tcggcttgca aagcagcgcg gctggccgat gatgaggtgg agctgatgcg
    11521 aaccctactg acactcgtga tcgttgcact catctgcggc gtcgccggcg cggacgtcta
    11581 caagagcaag gcgggccggg tctcgatcga ggttccgaag aagtggtccg tgatggcgca
    11641 ggacgacctc gtgcgcggcg cgtcgccgaa taacgaggtc gcagtcgtca tgtggatcgt
    11701 cgactcggcc gaggtcaaag atgcgctgaa gaagctcgag ggtgagctgt actcggcgat
    11761 ccagggcctg cggtggatcg acaagacgaa gaagctgaag gtgaacaaga ttgccggcac
    11821 ctgggtcgag ggcgtcggcg tgtcgagccg ctcgacgcag ctcgacgtca tcgcgttcgt
    11881 ggcggcgacg ccgacgaaga agggcgtcat cgtgctcgcg gtggtggatc atgacaaata
    11941 cgaggcgaat cgatcggcga ttcagtcgat tttcggaacg ctaaagacga cgaagtgatc
    12001 tcggcgccga cgagcccgcg cggaaagatt tcgggatcca tcgtcggacc gatcatgtaa
    12061 ggtgagcgcg tctatgggtg actttgctct gcgtggcctc gcagcgattc ctgcggtgcg
    12121 ccggctcatc gagctcgcgc tcgacgagga tctcggccgc ggcgatgtga catcgcaggt
    12181 cactctaggc aacgaaggtc gccgcgtcgt tgccgacatg aacgcgcgcg aaccgatcgt
    12241 cgcgttcggc gtcgacgtcg cggccgcggt gttctcgctc gtcgatccgc gcatcgaggt
    12301 cgaggtgctc gcgcccgatg gcacgcgcgt cgacaagggc gggctcattg tcacggtcac
    12361 cggtcctgcg cacaacgtgc tcgcggccga gcgcaccgcg ctcaatttca tccagcgcct
    12421 ctccggcgtc gcgacactgt cgaagcgcta cgccgatcac gttgcgggca cgaaggcgaa
    12481 agtcgtcgat acgcgcaaga ccacgcccgg ctatcgcgtg ctcgagaagg cggcggtgct
    12541 cgccggtggc tgcgcgaacc atcgcttcga tctcggctcg ggcatcttga tcaaggacaa
    12601 ccacatcgcg gcggccggtt cggtgcgcgc tgcggtcgaa gcggcgaagg cgcgtgcgcc
    12661 tcacccgcta cgagtagaag tcgaggtcac gaacctcggc gagctcgacg aagcgctcgc
    12721 ggcgggcgcc gagatcgtgc tcctcgacaa catgacgccg tcgcaagtcg aagttgctgc
    12781 agcgcgcgcg catggtcgcg gcgtgctggt ggaagtatcg gggggcatca cgctcgcgac
    12841 gatcgcggac tacgcgcgcg ccggagcaga tttgatctcc gtcggcgcgc tgacgcactc
    12901 ggcgcctgcg gtcgatatcg gattggatgt gcgcacatga cctttgaagc gctgcatctc
    12961 aaagcgcgct ggctggggtg ccagcgcatc gtgctcgact cgtgcggctc gacgaacgac
    13021 gaggccgcgc ggctcgcacg cgccggcgcg aagcacggca cgatcgtgat cgccgaacgc
    13081 cagaagagtg ggcgagggcg cgacggtcgc acgtgggagt cgccgcccgg cggtctgtac
    13141 atgagcgcgg tcgtgcgccc accgattcct gtcgctgacg tgccgccgat gacgctcgcg
    13201 atcggcatcg gcctctgcga agcagcgcgt acgacgggtg cggccgcggt gctcaagtgg
    13261 cccaacgatc tgctggtgcg tcatcgcaag ctcgccggtg tgcttgtcga ggcgcagagc
    13321 caggccaatc ggctcgaagc ggtgatcatc ggcatcggtg tcaacgtggg cggcgaattg
    13381 cccgagcctg tcgccgagtc aggcatcacg ctcgcggaag ctgccggcac gacgatcgat
    13441 cgcgacgcgt tcatcgagac cttgctcgcg cacgtcgagc gctgggtcga tcaatacgtg
    13501 tcggtcggca tcgagccgat cattccggcg tggcaagcgc gcatggcggt cggccttgct
    13561 gcgcgcgcga cggtcgatgg tgcatcgctc accggttgcg tagccgggtt ggacgcagat
    13621 ggcgctctgt tattgcgtga cgaagatggc aatgtgcatc gcgtccgttc gggcgatgtc
    13681 gaagtgttgc gcgctgcttg ctaactctgc atgccgcggc accgagccgt gtcgccgtgc
    13741 taccacctag gcatgcgtaa cctgctcgtg ttcggtggcc tcgctgtggt cgcgctctgc
    13801 gtcggcctgt ggctgctctt gcacaaaggc gatgacgcat cggccgagac cggcacgacg
    13861 caaggctcac aggccgtcgt cgcgccgccg catacgagcg gcacacccag cgtgacgagc
    13921 gccccgcccg gcgagcatcc tgcactcccg accgtgggct cgggcgaaaa tccgcgcgac
    13981 tacgtcgtcg gtgacattcg cgttcgcgat catcgcgcag gcgacaacaa gcccctcgac
    14041 attccaccga acgtgcaccc tccggaggga ccggcgattc catccgagct cacgcacgcc
    14101 gttgcacaaa aagtgaaagc cgtgatgatc gactgcgtcg cgagcctgcc gcaggacgcg
    14161 cgaggcgaaa agccgcgact cgagggtcag atcgagatct cgatcaagga tcataaggtc
    14221 cggatcgaga agtcgacgat gcagctgcgc aacgttagcg gcgaatcggt cgaaccaact
    14281 cgacaatgca tcgagtcgaa atccgttggc atcgagaatt ccgcgccaga tcaaagcgac
    14341 ctcgcgagtt atccgatcaa catctcgttc gcgattccgt gacgatccag tgacgttgcg
    14401 actgcggctg cgaaaagcgc cgagcaatgc gtcatgaagt gtcaccttgc cccgtcgcca
    14461 acgcgtagta gatgcgtgtt agacccgggc tcgagaacgg ttgatgaccc ccacctcggt
    14521 atgtatagtc ccgcgcctca tgagtcggac ctctgtccgc gcagtgcgct tgctgggaat
    14581 ggtcacgtta ctcgtggcca tcacatcgac gctgggatgt gagcagctcg acggccgcaa
    14641 tcgcaataag aagggcaatc gctacttccg cgagatgaag tttatcgacg cggtggcgga
    14701 gtacgagaag gcgctgaaga cggtagatga cccgatcatc cactacaacg tcgcgctcgc
    14761 gtactcgaag atcttcaagc cgggcatgga gaccgaccag gagctcctgc ttggtgagaa
    14821 gggcagcttc gtctgcgatc aagttccgca ggtgaagttc gtctccaagc aagtgtgcgt
    14881 gaagccgggc gatcgccgct acgacgcctg cgacgcgaag aacgtctgcg catcgagcta
    14941 cacgtgcaaa cagaccgagc tgtgcgcgat cggcaacaac gcgattgccg acatgaccgc
    15001 gcagcacttc ggcgtgtgga tcaaggtgca cccgaaggac gcagagacgc gcggcctgat
    15061 gacgcaagtc tggatggact cgggccagta caagaaagcg ctcgattact ggcagtcgct
    15121 gctgagcgac aagccgaacg acaccgagat catgggcgcg ctcgccggca tcaacttgaa
    15181 agctggcgac tggcgcaaat cgatcgagtg gtacacgaag gtcgccgagc tctcgaagga
    15241 agagtcggcc aaggtcgctg cctaccagtt catcggcaac gtcgcgtggt ccaagctcaa
    15301 ctccaagacg ctcaccacgg cggacagcgt cgagctcgcg gatcgcggca ttggcgcgct
    15361 gcagaaggcc gccgagatcc agccgaagaa ccccaagctc ttcggcctga tgggctccat
    15421 cttcaacttc cgctcgctgg ccatgggcgc ctcgtgggca gccggcctcg atcgcgcaac
    15481 tgcgcaagat cttcagatcg tctcgcgcgt tcttagcgat gaggcgaaga aggctcaagg
    15541 tctacccgtg actccgaccc cggcgcccgc acccgcaccg agtcccgcgc caagcggaag
    15601 cgccgctcct gcaaccccta gcccggccaa gaccggcggc tgattcgccc agaaggagag
    15661 tagctgccga tgttcgctaa ttacgccgcc gcaaagaacc gcaagctgcc gaagtgggcc
    15721 ggaccccttc tggtcggcgc agtcatcttc cacatcggac tgttcgtcac gatgtggatc
    15781 aagtcgatct gggacatcga gctcctcgag cgtcccaaga cgacgatcga tctcgctgtc
    15841 gctccgccgc cacctcctcc gcctccgccg cctccgggtg gtgcgaagcc gcaggaagtc
    15901 cggatcgagc cgaagaagat caaggtcaag gacatcgtcc agccggtcaa gatcgagaag
    15961 caggtcgctc aggtccagga caccggtccc gccggcgtcg aaggtggtgt cgagggcggc
    16021 gtcgagggtg gtgttgtcgg tggcgatctc aacggcgtga tgggcgcgcc gcctccgcct
    16081 ccgccgcctc cgcctcctgc accgccgcag aacgtgccgc cgacgctcct cgaaggctcg
    16141 cgtatcgcgg gcgagaagat gatcagcccc gacgacgtga ccaagaccga gatccaacgc
    16201 tcgggcaagg accgcatcat cggcagcttc aagctgtgtc tcaccgtcac cggtgcgatc
    16261 agcaacgtga acatgctgaa gtcgacgggc ttccccgcgt acgacggcaa gatccagaac
    16321 aagatgcgct ctgagtggcg ctacaagccg tacatggtca acggtaaggc cgttcccgtg
    16381 tgcaccgccg tgacctttat ctactcgcag aagtaaccaa caggccaaga ggaaagtgca
    16441 gctatgggac tcgacctaat ggagctctgg gaacacatga gcatcgtggt gaagatcatc
    16501 acggtgctga tggtcctcat gtcggtgttc atgttctaca tcatcgccga gcgtcttctg
    16561 acgttccgcg cggcgagcga tcagtcgatt cgctacgtgc aagcgttggg tgagtacctg
    16621 cgccagcaca aggtgaacga ggcgctcacg gcggcgcgcg gcgcgaacaa gagccccgtc
    16681 gcgaaggtga tggagtcggg cctcgcagcg ctgctgcagg gtcgcgaagc tctcaccacc
    16741 cagggtcccg ccgacgtcgg tgacttcgat ctcgtcgact ccgtcaatcg cgcgctcgag
    16801 cgcgtgaagg agcgcgagac gagcaacctg cgccgcggcc tcgcgttcct cggtacgacc
    16861 gcatcggcga cgccgttcat cggtctgctc ggaacggtcg tcggcatcct cggtaccttc
    16921 cagatgctca agggcggtac cgtgacgatt aacgagatcg gcccgaagat ctccgaagcg
    16981 ctcgtctcga cggcgctcgg tctcggcgtc gcgatcatcg cggcgatgtc gttcaacttc
    17041 ttcacgtcgc gcgtcgagca gttcgtgatc gacatgaacg acgtgtcgag cgagttcatc
    17101 gactacgtgt tgcgcgaggg tcggagctag tcaccgtggc cggcgcgccc caaaagaaca
    17161 gtggcaatgc gggtcacccg catcgccgct ggcgcctgtc gggcaagaag catcacatcg
    17221 attcggttcg caacgacatc aacgtgacgc cgctcgtcga cgtcatgctc gtgctcctca
    17281 tcatcttcat gctgatgacg ctgatcatgg gtcgcggcca cgacgtgaac ctgccgaagg
    17341 cggcgaacta ctcgcaagag aaagacaaga tgcagcccgt ggtcaccatc gacctcgagg
    17401 gcaacctgtt cgtcgagaag aacaagctcg gtcctgtcaa cgagtccacg ctgaaggaga
    17461 tggccaacca gatccaggcc gcgtgggcag cgcccaagaa ccccgacggt gtcggccgca
    17521 tctacatcaa ggccgatagc aaagtcgggt acggcaaggt ccaccccgtg ctgatgtatc
    17581 tgaaccgcga gctccagctc cagcagatcg acctcgcgat cgcgaaggac agcaaggagg
    17641 gcgactagcc atgggcatgt caaccggctc tggcgacaag cgcgccgtcc agagcgaaat
    17701 caacgtcacg ccgctgatcg acgtcctcct cgtgctcctc atcatcttcc tcgtcgtcat
    17761 gccgatcatg atgaagatgg agacgctgca ggtgccgcgt aagctcgacg atcagaacga
    17821 gatgcccgat ccgaacgcga gccaattgac cgtcaaggtc aagaacgacc tctcgatcgc
    17881 gttcaacgac ggcagctcgg agaacgacat gccgatcaag gcggtcgaca tcgcatcgac
    17941 gctacgtccc aagctcgaag cgatgcaggc caaccaagag aaggtcgtgt tcgtcgagtt
    18001 cgaagaaggc gtcccgtggt ccgatgtcgt cggtacgatg gacacgatcc gcagcctcgc
    18061 tgccgacgtg aaccacgacg acatcaaggt cgcgttgaag atcaaagagg acgagcagaa
    18121 gcagtagcgc tttggccgtg cgacaagacg cagcccccct tgtggctgcg ttttgcagtt
    18181 tcgggccgcg tttcctcgtg cagatcgctg cgactgcgac agcgacgcgg gtcgcgtgat
    18241 gcaacgcggc ttgatcgggt cctgttagct gcgccgcgtc gtcggtgata gccgtgacct
    18301 atggcgttca cacagcgggg ctcggctgag atcaatgtca ctccactgat cgacgtgctg
    18361 ctggtcctgc tcgtgatttt tctgatcgtg atgccgacga tggtgaagtt cgaggaggtc
    18421 cacacgccgg cgccgccgga gcagagcggc gagtacgtcc agagcctcgt cgtgcgcgtc
    18481 cacgccgata ccagcgtcgt cctcgtcgac gaaggccgcg aaatcccgct cgatgcgggc
    18541 ggccagctgg ctggcgggct gcgctcgcat ctgcctgcgc ggacgcaggt cgtgttcctg
    18601 gagctcgagg acgacgtcca gtggggccac gtcgtgtcga cgatcgacac gattcatggt
    18661 gtcgcaccga tgactccggt cgcattgaag gtcgcagacg aatgagggtg cgacacttcc
    18721 agcgaaccgg actcggcgac cccgtttggg ctgtcacgtc cggtgatcgg aagtccggtt
    18781 cagcgaggga tccggcggtg atacaaggcg atgaaccgcc ttgacaccaa tattcctcgt
    18841 tcagtacaag cgggcgactt ttgcgtcgtt gtgcacccgc gatgaagggg agcagtgaga
    18901 cttatgactt cgagtcgatt tggaattgca gtcgtccttg cttcgctatc ggttgccgcg
    18961 tgcaccgatc tcgatagcgc aacgaatctc aaccctgaag gcccgccgat gatccggcag
    19021 gttcgcctga aggagaaata tcaggacgcg accggcgtgc tgaccgcgcg cgggaatccc
    19081 gtgttcgcgt tcggcacgca cccgcagatc acggggccgg atgacgcgca cccggtgacg
    19141 tcggcgactg ccgtcggcaa caagctgcgc atcatcatgg acgagctgct cgtcggtaat
    19201 aacctgcagc agatcgagtg tcgccagccg gtcaacaccg acggcgcgtt cgacagcgtg
    19261 ccgatcggcg cgacgcctga cgatgtcgcg cgttgctcgg tcgcgtcgga cgtgttgccg
    19321 aagtcgtgcc cggcgtcgaa cccgcactcg atgtgcatct gccagatcgc gacgggctgc
    19381 ggtgtcaccg gcgaaatcaa gatgaacatg ccggtcggtg tgaaggatgt gaaccaggac
    19441 ggcgctgccg acggcatgcg catgatgccg aactcggtcg gtctcaagtg cggctcgatc
    19501 gacgtgccga tcgatctcaa caacagctac tggaatccgt ccggcgatca gaacgtgccg
    19561 gcgatgggtg gcttcgacgc gctcggcccg gcgatcgtgc tccagccgga tcacccgctc
    19621 ggcatggcgt cgattccgca gttcctgccc acgaacacca cgtgcagtct cgtgttcgat
    19681 ccggccgtgg tcgacaagtc gggcatcaag gtctgcgcgc cgcccgacgg cgacatcacc
    19741 aagaactgca cgccgggtaa cgtcgacgcg ttcacgttcc gcgtcgagcc gctcgcgatc
    19801 gacaacgctc tgtcgaacgt ccacgacggt gacacgggcg tcagccgcac ggacggcgtt
    19861 cagctgtaca tgaccgcacc ggtctcggca gcgagcatca gcgcgatcac cgttacggaa
    19921 ggcagcacga gcttcacggc cttcacggcc acacagtcgg ctcccgatca aatcaagttg
    19981 acgtggacgg gcgcgaccgg gctcgcggct aacacgatgt acacgatcac cgtcggcacc
    20041 ggactcaccg atgtctacaa ccaggccgcc ccgatgcctt ccaccatctc cttcatgact
    20101 ggatcgtgag gattgccatg aagactcgca aactccttct tgccgcgacc ctgggctcca
    20161 ccagcctgtt cgtcgcgaat ctcatcaacc ccgacctcgc ggcggcgcaa agctcgaccg
    20221 caggcgcgat tcaaggtgtc gtcactgacg gcaagtccgg cgagaagctc gctggtgtga
    20281 ccgtgatcgt gacgtcgccc gcgctggcgc agacgcagac cgcgatcacc gacgagaacg
    20341 gagcgtacca agtcggcgcg ctgccgcccg gcgagtacct ggtcacgttc tactacgccg
    20401 acatcaccgt cgagcgcagc ggcatccgtg tcggtatcga caagacgacg ccggtttacc
    20461 agaagctgaa catggcagcg gctggtggcg agacgatcaa gatctcggac accgcgccga
    20521 cgatcgaccc gacgtcgacg acgcagggca tcacgatcga caagaactac ct
//
LOCUS       DASUFX010000337         6005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_52604,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000337 DASUFX010000000
VERSION     DASUFX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52604"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFV99_13570"
     CDS             <1..538
                     /locus_tag="VFV99_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060389.1"
                     /translation="VGEMGSILRFDGTTWMPMTSNTVRTLIAIWGAKATTSTSATTIV
                     AAGAAGTVQRYDGSDWQAAPNTPLNGLTLRGVWTSGTRTVAVGINSSSMAVIATTEAA
                     GWNTQTASPMYLQSVWGVADDNIYAVGAGGTIMQFDGARWMPMTSGTQQPLQAVSGTT
                     TNVYVVGNVGTILRLGVN"
     gene            complement(535..2178)
                     /locus_tag="VFV99_13575"
     CDS             complement(535..2178)
                     /locus_tag="VFV99_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060390.1"
                     /translation="MRGLWSTATLCAITASASLLAACASDGDDPSSVELALGGQAAVK
                     LTNSNAHMALTSDTQWTLDKQGSVNTSTHTVTWTITATKGMTQTGILIVDGTMTVKNT
                     GSGGATIGNIVVNLQTRSGNTWVTKSSDIANATQDDAATSAKVVSSASSENKSTFNEN
                     SASGHLLFMDAQTNSAFALVPQVTIPGGTTKKLVFKATFDNNVLHLSAGTSTRVEMIL
                     SFGNAGGGASKPNIDINGNGLIDPDEARVRSVPSRLGLTVPHTTPANSTATISDALSD
                     ITKTGTVTFTNPQFNLGTTTGTVTVHYDGGTSGGTITNCAHLKSQTQNATCGGHTFNN
                     VLGVNLTDCNTQVIGPHTCSPGLPGCGWETGDVVTYTQDGWGEPASIPGGLLVANFDN
                     IYINALEVGIAGTAGFSMRFFDALAVFDYAPASGANGPLTGDLVDPTSSSAGLFGGNV
                     MALQLDVDFSDAGVLGGTSGLRFGDLRMCNFTTLSALNGMTVRQYLAVVNTLLGGGTG
                     PYSIDDLNPVTFALTQAFLGGAPSTFAQQQLFNSASCPI"
     gene            2533..3000
                     /locus_tag="VFV99_13580"
     CDS             2533..3000
                     /locus_tag="VFV99_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX5060391.1"
                     /translation="MDQALVTIASGHPLIDIDWTVAVQFGLFLLLFYVSNKLLFQPYL
                     RLRERRKEGIDGARAEAERMTAQADAKLADYDKHLAVAKNRANEEGRKVRTEAAAHEK
                     DVTDKARAEAQKSIDEATAKMRQETDAARLQLLPQANQLARQIASKLLGREVS"
     gene            3000..3734
                     /locus_tag="VFV99_13585"
     CDS             3000..3734
                     /locus_tag="VFV99_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX5060392.1"
                     /translation="MKKLLIAALLCAPAVAFAQPDEPKVESTTHAAAEIEPQPHDPTA
                     HFNFTNFDFRGKDEYGGKYGDGEMTDAKTGHTTREEEPMSAPFVLMLLNFGVLLLILA
                     KWGGPVARKLAQDRHDQIKSALDEAAKLRDQAATKLAEYEAKVKGLDNEVKQIVDGIR
                     ADAEADKKRILESATAQAAQMKRDAEQRIAAEIEYARKALQKEVTVAASTATEKLLKD
                     KVTGDDQNKLVSTFITNVQNGRKEAR"
     gene            3734..4273
                     /gene="atpH"
                     /locus_tag="VFV99_13590"
     CDS             3734..4273
                     /gene="atpH"
                     /locus_tag="VFV99_13590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX5060393.1"
                     /translation="MVTGSLARRYAKAIFDIGSQQGDLAKLGQDLRSLAKAMNESKEL
                     GTALTNPAIRRSDRKKVIDGLLQSIGVQTASRNLVYILLEGERMGTVPGISRALDEMI
                     EAKAGRVTAEVVSAKPLGSDQLSQINAALEKLSGKKVSVTTRQDPELLGGVIAKVGDT
                     VYDGSLRTQLRTLRDELSK"
     gene            4290..5822
                     /gene="atpA"
                     /locus_tag="VFV99_13595"
     CDS             4290..5822
                     /gene="atpA"
                     /locus_tag="VFV99_13595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5060394.1"
                     /translation="MDIRAAEISDIIRKQIENYDKKVSVTETGTVLTAGDGIARVYGL
                     SGALAGELLEFEGAGGETVQGMVLNLEEDNVGVALLGKFEAIREGDTVKRTGRIVEVA
                     VGEELLGRVVNAMGQPIDGGKPVVGTHRRQVEIKAPGIIARKSVHEPMQTGMKAIDSM
                     IPIGRGQRELIIGDRGTGKTAIAIDTIINQKGQNMYCFYVAIGQKQSTVATVVDRLTK
                     AGAMEYTTVVVAGASEPAPLQFIAPYTGVTMAEYFRDSGRHALIVYDDLSKQAVAYRQ
                     LSLLLRRPPGREAYPGDVFYIHSRLLERAAKMSDAKGAGSLTALPIIETQAGDVSAYI
                     PTNVISITDGQIFLEADLFFSGVRPAVNVGISVSRVGGAAQTKAMKKIAGRLRLELAS
                     YREKAAFAQFGGDLDKATLQQLARGERMVELLKQGQFVPMPMEEQVVVIYAGTNGFVD
                     DYPVSVLGRYEKELLSFLKSRKADLLAELKSTGKLEGDTEKQLRDALTEFAKQFSTEE
                     KK"
BASE COUNT         1122 a         1967 c         1953 g          963 t
ORIGIN      
        1 cgtcggcgag atgggctcga ttctccgctt cgacggcacg acgtggatgc cgatgacgtc
       61 gaacacggtg cgcactctca tcgcgatctg gggcgcgaag gccaccacca gcacgagcgc
      121 cacgaccatc gtcgcagcag gcgccgccgg tacggtccag cggtacgatg gctcggattg
      181 gcaggccgcg ccgaacacgc cgctcaacgg cctcacgctg cgcggcgtgt ggacgagcgg
      241 aacgcggacc gtcgccgtcg gcatcaactc atcgagcatg gcggtcatcg cgacgacaga
      301 agcggcggga tggaatacgc agaccgcgtc accgatgtac ctgcagagcg tgtggggcgt
      361 cgccgatgac aacatctatg cggtcggcgc cggcggcacg atcatgcagt tcgacggcgc
      421 acgctggatg ccgatgacct cgggcacgca gcagccgctg caagcggtca gcggcacgac
      481 cacgaacgtc tacgtggtcg gcaacgtcgg cacgatcttg cgcctgggcg tgaactagat
      541 cgggcagctc gcgctattga acagttgctg ctgcgcgaac gtcgacggtg cgccgccgag
      601 gaatgcttgc gtcagcgcga acgtcaccgg gttgagatcg tcgatcgagt acgggccggt
      661 gccgccgccg agcagcgtgt tcacgactgc gaggtactgg cgcaccgtca tgccgttgag
      721 cgctgacagc gtcgtgaagt tacacatgcg gagatcgccg aaccgcaggc cggaggttcc
      781 gccgagcacg cccgcatccg agaaatcgac gtcgagctgc agtgccatga cgttgccgcc
      841 gaacaagccc gccgacgacg atgtcggatc gacgaggtca ccggtgagtg gcccgttcgc
      901 accgctcgcc ggcgcgtagt cgaacaccgc gagcgcgtcg aagaagcgca tcgagaaccc
      961 ggccgtgcct gcgatgccga cctcgagtgc attgatatag atgttgtcga agtttgcgac
     1021 gagcaggccg cccgggatcg acgccggctc gccccaacca tcttgcgtgt acgtgacgac
     1081 gtcgccggtt tcccagccgc atccgggcag gcccggcgag caggtgtgcg gaccgatcac
     1141 ctgcgtgttg cagtcggtga ggttcacgcc gaggacgttg ttgaacgtat ggccaccgca
     1201 cgtcgcgttc tgggtctggc tcttcagatg cgcgcagttc gtgatcgtgc cgccgctcgt
     1261 gccgccgtcg taatggacgg tgaccgtgcc ggtcgtggtg ccgaggttga actgcgggtt
     1321 cgtgaacgtg accgtgccgg tcttcgtgat gtccgacagc gcgtcgctga tcgtcgcggt
     1381 cgaattcgcc ggcgtcgtgt gtgggaccgt gaggccgagc cgcgacggca cgctgcgcac
     1441 gcgcgcttca tctggatcga tgaggccgtt gccgttgatg tcgatgttcg gcttgctcgc
     1501 accgccgccc gcgttgccga acgacaggat catctcgacg cgcgtcgacg tgccggcgct
     1561 gaggtggagg acgttgttgt cgaacgtcgc cttgaacacg agcttcttcg tcgtgccgcc
     1621 cggaatcgtg acttgaggaa ccagcgcgaa cgccgagttc gtctgcgcgt ccatgaagag
     1681 gagatgaccc gacgccgagt tttcgttgaa cgtgctcttg ttctccgagc tcgccgatga
     1741 cacgaccttc gcgctcgtcg ccgcatcatc ttgtgtggcg ttcgcgatgt cggacgactt
     1801 ggtgacccag gtatttcccg atcgggtctg cagattcacg acgatgttgc cgatcgtcgc
     1861 gccgccgctg ccggtgttct tgaccgtcat cgtgccgtcg acgatcagga ttcccgtctg
     1921 ggtcatgccc ttcgtggcgg tgatcgtcca cgtcaccgtg tgcgtcgacg tgttcaccga
     1981 gccctgcttg tcgagcgtcc actgggtgtc gctcgtcagc gccatgtgcg cgttcgagtt
     2041 ggtcagcttg accgcggctt gcccgccgag agctagctcg accgacgacg ggtcgtcacc
     2101 atcgcttgca caggctgcga gcaagcttgc acttgctgtg atcgcgcaca gcgtcgccgt
     2161 cgaccaaagg cccctcatcg agttggacta tatgcaggca gcctgcccgt ccggttacgt
     2221 tgccttacgc caaatgctga cagcgaatgc ggttcacaga ccgcatctgt cgcagggctc
     2281 gggtgcgatg gccgctacat cgatcgatcg ctgacggccg gcatcgaccg aaagacccgg
     2341 tgggttcgac cgactgatcg gtcgggatcg agctccatcc gacgatgatc gcggcctccg
     2401 atctcgtcgc caacgatcgc ggcgcattgt cgcagcaccg tcgagaatcc tggcgcatcc
     2461 caacgttgac acggtcggac acggagacta taagcagccc gccccaaggc caggattctc
     2521 gctaggttgt ccatggatca ggcactcgta actatcgcgt caggacaccc gctgatcgac
     2581 atcgattgga cggtagccgt tcagttcggc ctgttcctgc tcctgttcta cgtgagcaac
     2641 aagttgctgt tccagccgta cttgcggctg cgcgaacggc gcaaggaagg catcgacggc
     2701 gctcgcgccg aggccgagcg gatgaccgcc caggccgatg ccaagctcgc cgattacgac
     2761 aagcacctcg cggtcgcgaa gaaccgcgcc aacgaagaag ggcgcaaggt gcgcaccgag
     2821 gcggcggcgc acgaaaagga cgtcaccgac aaggcccgcg ccgaggccca gaagtcgatc
     2881 gacgaagcca ccgcgaagat gcgccaggaa actgacgcgg cgcggctcca gctgctcccg
     2941 caggccaatc agctcgcgcg gcagatcgcc agcaagctgc tcggccggga ggtttcgtga
     3001 tgaagaagct cctcatcgca gcgctgctct gcgcaccggc cgttgcgttc gcgcagccgg
     3061 acgagcccaa ggtcgaaagc accacgcacg cagctgccga gatcgagccg cagccgcacg
     3121 atccgaccgc gcacttcaac ttcacgaact tcgacttccg cggcaaggac gagtacggcg
     3181 gcaagtacgg cgacggcgag atgaccgacg cgaagacggg ccacacgacg cgcgaagagg
     3241 agccgatgtc ggctcccttc gtgctgatgc tgctcaactt cggcgtcctg ctcctcatcc
     3301 tcgcgaagtg gggcggaccg gtcgcgcgca agctcgcgca ggatcgccac gatcagatca
     3361 agagcgcact cgacgaagcc gccaagctcc gcgatcaagc ggcgacgaag ctggccgagt
     3421 acgaggcgaa ggtgaaaggc ctcgacaatg aggtgaagca gatcgtcgac ggcattcgtg
     3481 ccgacgccga ggcggacaag aagcgcatcc tcgagtccgc gactgcgcag gccgcgcaga
     3541 tgaagcgcga cgccgagcag cgcatcgccg ccgagatcga atacgcgcgc aaggcgctgc
     3601 agaaggaagt cacggtcgcc gcgtcgactg cgaccgagaa gctgctcaag gacaaggtga
     3661 ccggcgacga ccagaacaag ctggtgtcga cgttcatcac caacgtgcag aacggccgca
     3721 aggaggcccg ctaatggtga ccggcagcct cgcacgtcgt tacgcgaagg cgatcttcga
     3781 catcggatcg cagcaaggcg atctcgcgaa gctcggccag gaccttcgtt ctctcgcgaa
     3841 ggcgatgaac gaatcgaagg agctcggcac cgcgctcacg aacccggcga ttcgccgcag
     3901 cgatcgcaag aaggtgatcg acggcctgct ccagtcgatc ggcgtgcaga ccgcatcgcg
     3961 caacctcgtc tacatcctcc tcgagggcga gcgcatgggc accgtgcccg gcatctcgcg
     4021 cgcgctcgac gagatgatcg aagcgaaggc cggtcgcgtc accgccgagg tcgtctcggc
     4081 caagccgctc ggctccgacc aactctctca aatcaacgcc gcgctcgaga agctatcggg
     4141 caagaaggtc agcgtcacga cgcggcagga cccggagctg ctcggcggcg tcatcgcgaa
     4201 agtcggcgac accgtctacg acggctctct tcgtacccag ctccgcaccc tccgcgacga
     4261 gctcagcaag tagcaagtaa ggacacgcca tggatatccg cgccgcagag atctccgaca
     4321 tcatccgcaa gcagatcgag aactacgaca agaaggtctc ggtcaccgag accggcaccg
     4381 tgctcaccgc cggtgacggt atcgctcgcg tctacggcct ctccggcgca ctcgccggcg
     4441 agctcctcga gttcgaaggt gccggtggcg agaccgtgca gggcatggtg ctcaacctcg
     4501 aagaggacaa cgtcggcgtc gcgctcctcg gcaagttcga agcgattcgc gaaggcgaca
     4561 cggtgaagcg caccggtcgc atcgtcgagg ttgccgtcgg tgaagagctg ctcggccgcg
     4621 tcgtcaacgc gatgggccag ccgatcgacg gtggcaagcc ggtcgtcggc acgcatcgcc
     4681 gccaggtcga gatcaaggcg cccggcatca tcgcccgcaa gtcggtgcac gagccgatgc
     4741 agaccggcat gaaggcgatc gactcgatga ttccgatcgg ccgcggccag cgcgagctga
     4801 tcatcggcga ccgcggtacc ggcaagacgg ccatcgcgat cgacacgatc atcaatcaga
     4861 agggccagaa catgtactgc ttctacgtgg ccatcggcca gaagcagtcc acggtggcca
     4921 ccgttgtcga ccgcctcacg aaggcgggcg cgatggagta cacgacggtg gtcgtcgccg
     4981 gcgcgtcgga gcccgctccg ctgcagttca tcgcgccgta caccggcgtc acgatggccg
     5041 agtacttccg cgactccggc cgccacgcgc tgatcgtcta tgacgatctc agcaagcagg
     5101 ccgtcgcgta ccgccagctg tcgctgctcc tccgccgccc gccgggccgc gaggcgtacc
     5161 cgggcgacgt gttctacatc cacagccgcc tcctcgagcg cgccgccaag atgagcgacg
     5221 cgaagggcgc gggctcgctg acggcgctgc cgatcatcga gacccaggcc ggtgacgtgt
     5281 cggcgtacat cccgacgaac gtcatctcga tcaccgacgg tcagatcttc ctcgaagcag
     5341 acctgttctt ctcgggcgtt cgtcccgcgg tcaacgtcgg catctcggtg tcgcgcgtcg
     5401 gtggtgcagc gcagacgaag gcgatgaaga agatcgccgg tcgcctccgc ctcgagctcg
     5461 cgagctaccg cgagaaggcc gcgttcgcgc agttcggtgg tgacctcgac aaggcgacgc
     5521 tgcagcagct cgctcgcggc gagcgcatgg tcgagctgct caagcagggc cagttcgtcc
     5581 cgatgccgat ggaagagcaa gtcgtcgtga tctacgccgg caccaacggc ttcgtcgacg
     5641 actacccggt ctccgtcctc ggccgctacg agaaggagct gctgtcgttc ctcaagagcc
     5701 gcaaggccga tctgctcgcc gagctcaagt cgaccggcaa gctcgagggc gacaccgaga
     5761 agcaactgcg cgacgcgctc accgagttcg cgaagcagtt ctcgacggaa gagaagaagt
     5821 aacgcgccat gccgagcctg aaggcaatcc gcaagcgaat cgtgagcgtg aagaacacgc
     5881 gcaagatcac gcgtgcgatg aagctcgtcg cggctgcgaa gctgcgccgc gcgcaggaca
     5941 cgatcatcgc ggcgcgcccg tactcggccg ctctgtcgtc ggtcgtctcc gacctgtcgg
     6001 gcgtc
//
LOCUS       DASUFX010000338         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_887371,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000338 DASUFX010000000
VERSION     DASUFX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887371"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFV99_13600"
     CDS             complement(<1..251)
                     /locus_tag="VFV99_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060395.1"
                     /translation="MSNGRDANSELEQREWDAQESALRAECAGSWAGGGADVAQYRVI
                     ARALRTPQLDAIPRDFAAETAARALREARLASESVEVWL"
     gene            complement(248..877)
                     /locus_tag="VFV99_13605"
     CDS             complement(248..877)
                     /locus_tag="VFV99_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060396.1"
                     /translation="MGSGEPAVIEASGIGSSEPDAVDTDRRLVAAVLAGTRGAFETLV
                     LNHQRLCWHIINRMVRHPDDTRELCQDTFLRVHRYLYQYRFESALKTWIGQIAYSVAR
                     RHLERKRIPIVEPAADDDSGSFVERVADDFNLEAANADAELGAMLHAEIDALPPLQRT
                     VLTLYHLDELPIAEIAGITGLSAGTIKSHLFRCRARLRGRLQARWGVKE"
     gene            1015..1344
                     /locus_tag="VFV99_13610"
     CDS             1015..1344
                     /locus_tag="VFV99_13610"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX5060397.1"
                     /translation="MRFEVLIPITLFFCITYAIKLLVDARTRSKLIAANGSEELIRSL
                     VQNEEQQRRHASVRWGVVLVCLAVGFALIEFFGWDDVTPGAIAVLLGATGVGNLVSFY
                     ISRDLPK"
     gene            1350..>2451
                     /locus_tag="VFV99_13615"
     CDS             1350..>2451
                     /locus_tag="VFV99_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060398.1"
                     /translation="MNYIILIGIALFFVLTAIRNGDAGWDFRNNALNFRISDGDYSLR
                     VKGEGDVDLKPDGSGLASLDRGGSFDITLRRNGVDRRVLFTRDDATIERKFFVDGDEQ
                     AWDPEADRFVADVMPIVLRETAINAGERVAWLIDNRGQNGLLDEIELIHSDFAQRVYS
                     VQYAKTATIAPADFERLMKLTADNMSSDFDVRTTLIEAFDAQMPTGASFDALLRAGET
                     ISSDFDARMVLEHVGSSMQRTAEAGTAYLNLARTISSDFDMRLALQPLLTSSDSDDDV
                     VARALDLARNDISSDFDLRTVLSEASGRVGQSDTLARAYTTASRSISSDFDQRLALSA
                     LAANAELTPAGWQMLLEAATDISSDFDAATLLT"
BASE COUNT          420 a          832 c          760 g          439 t
ORIGIN      
        1 ctgagccaca cttcgacgct ctcgctggcg agacgagcct cgcgcaacgc acgagcagcc
       61 gtctctgctg cgaagtcgcg cgggatagca tcgagttgcg gcgtgcgcag ggcgcgcgct
      121 atgacgcggt attgcgcgac atcggcgccg ccgcccgccc agctgcccgc acactcggca
      181 cgcaacgcgc tttcctgtgc atcccattcg cgctgctcga gctcgctgtt cgcatcgcgt
      241 ccgttgctca ttccttcact ccccagcgcg cctgcagccg gccgcgcaag cgcgcgcggc
      301 agcgaaacag gtggctcttg atcgtgccgg ccgagagccc ggtgattcca gcgatctcgg
      361 cgatcggcag ctcatccaag tgatacaacg tcagaaccgt ccgctgcaag ggtggcagcg
      421 cgtcgatctc ggcatgcagc atcgcgccga gctcggcgtc ggcgtttgcc gcctcgaggt
      481 tgaagtcgtc ggcgacacgc tccacgaagc tgcccgagtc atcatccgcg gccggctcga
      541 cgatcgggat tcgcttgcgc tcgaggtggc gccgcgcaac cgaatacgcg atctgcccga
      601 tccacgtctt cagggcgctc tcgaaccggt actggtagag atagcgatgc acgcgcagga
      661 acgtgtcctg gcacagctcg cgcgtgtcgt cggggtgccg gaccatgcgg ttgatgatgt
      721 gccaacagag ccgctgatgg tttaggacca acgtctcgaa tgcgccgcgc gtgccggcca
      781 atacggcggc caccagacgc cggtcggtat cgacggcgtc tggctcgctc gaaccgattc
      841 cgctggcttc gatgaccgct ggctcccctg aacccatccc acgatcgata cgcccaacgg
      901 gccatcggtt gcaaccccag ccaagcggga tttctcgcaa ccggctcctc agaacccgta
      961 tctactgctt tcgaggccag ccatatcggc cgggctattg aggcgagcaa tcacatgcgt
     1021 ttcgaagtac tgatcccgat caccctgttt ttctgcatta cctacgccat caagctgctg
     1081 gtcgacgcgc gcacgcgcag caagctgatc gctgcgaacg gctccgagga gctaattcgc
     1141 tcgctcgtgc agaacgagga gcaacaacgc cgccacgcct ccgtgcgctg gggagtcgtg
     1201 ctcgtgtgct tggcagtggg cttcgcgttg atcgagttct tcggctggga cgacgtcacg
     1261 ccgggcgcga tcgccgttct gctcggcgcg actggggtcg gcaatctcgt ctcgttctac
     1321 atctcgcgcg acttgcccaa gtagggccca tgaattacat catcctgatc ggcatcgcgc
     1381 tgttcttcgt cttgaccgcg attcgcaacg gcgacgcggg ctgggacttt cgtaacaacg
     1441 cgctgaactt ccgcatcagc gacggcgact actcgctacg cgtgaagggc gagggcgatg
     1501 tcgatctcaa gcccgacggc agcggcttgg cctcgctcga tcgcggcggc tcgttcgaca
     1561 tcaccttgcg ccgcaacggg gtcgaccgtc gcgtcctctt cacgcgcgac gacgccacga
     1621 tcgagcgaaa gttcttcgtc gacggcgacg agcaggcgtg ggaccccgag gccgaccgct
     1681 tcgtcgccga cgtcatgccg atcgtgctgc gcgaaaccgc gatcaacgcc ggcgagcgcg
     1741 tcgcgtggct catcgacaat cgtggccaaa acggcctgct ggacgagatc gagctgatcc
     1801 actcggactt cgcgcaacgc gtctattcgg tgcagtacgc gaagacagcg acgatcgcac
     1861 cggcggactt cgagcggctg atgaagctta cagccgacaa catgtcgtcg gacttcgacg
     1921 tgcgcacgac gctgatcgaa gcattcgacg cacagatgcc gacgggagcc agcttcgacg
     1981 ctctgctccg cgccggcgaa acgatctcgt ccgacttcga tgcgcgcatg gtgctcgagc
     2041 acgtcggctc gagcatgcag cgcacggccg aggcggggac agcctacctc aacttggcgc
     2101 gcacgatttc gtcggacttc gacatgcggc tcgcgctgca gccgctgctc acgagcagcg
     2161 actccgacga cgatgtggtc gcacgcgcgc tcgacctcgc gcgcaacgac atctcctcgg
     2221 atttcgacct gcgcactgtg ctctccgaag cgtcggggcg tgtcggccaa tcggatacgc
     2281 tcgcgcgtgc ctacacgacg gcctcgcgct cgatcagcag cgatttcgat cagcggctcg
     2341 cgctatcagc gttggcagcc aacgccgagc tgacgccggc gggctggcag atgctgctcg
     2401 aagcggctac tgacatcagc tcggacttcg acgccgcgac gctattgacc g
//
LOCUS       DASUFX010000339         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1582429, whole genome shotgun sequence.
ACCESSION   DASUFX010000339 DASUFX010000000
VERSION     DASUFX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582429"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="VFV99_13620"
     CDS             complement(<1..2151)
                     /locus_tag="VFV99_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5060399.1"
                     /translation="MDAPDGGPEEIVCENLPPLASGTCEVTSVGAAQLIKGNILTPQT
                     IFRGGQVAIDATGEITCVGCDCAKGGETTIVCPGATVSPGLINTHEHITFQQAEPYTD
                     TGERYEHRHQWRKGLDGHTKIPSTGGASGVKISYGELRYVMGGATSIVGSGGSPGLLR
                     NLDNTSNQEGLNHTAVNFETFPLGDSSGTMRTADCNYGTTVTPESLSNVNSFEPHTSE
                     GINAAANNEFKCESSDTYDTMAPGLSENLLLPKTAMIHAIGLTPNDYGAMAVAGSALI
                     WSPRSNITLYGDTARVAEAARLGVEIALGTDWMPTGSMNMLRELHCADELNATYFDHY
                     FTDKQLWAMATLNAAAVTATDDAIGMLAPGHVADIAIFAANGKDAYRAIIEAEPKDVA
                     LVMRGGKALYGDATAVNELRSECDEVDVCGNDKRVCVKPEIGKTWDELKTAVGTIYPA
                     FACGTPAKEPSCSPKRPMAVNGSTVFTGQTSADDNDGDGIPNASDNCPDTFNPVRPMD
                     NGMQPDADGDGQGDACDVCPLDAGSTTCTNADPNDRDHDGVTNTADNCPDVANPNQTD
                     GDMDGHGDACDACPSDPNPGTAGCPASIYQIKASIVNTGSVVRVQNALVTGKGSNGFF
                     VQVKEGDMGYMGPANSGLFIYTGPMAPPLASAMVGLRFTIDGRVTSFQGQIELDTISA
                     VIPETMMGEAAPAPIGVTYTEVKTGGSRATELESV"
BASE COUNT          297 a          706 c          729 g          429 t
ORIGIN      
        1 cacgctctcg agctcggtcg cgcgcgagcc gccggtcttc acttccgtgt acgtgacgcc
       61 gatcggcgcg ggtgctgctt cgcccatcat cgtctcgggg atgaccgccg agatcgtgtc
      121 gagctcgatc tgaccctgga agctcgtgac cctgccgtcg atcgtgaagc ggaggccgac
      181 catcgcgctt gccagcggtg gtgccatcgg accggtgtag atgaacaggc cggaattcgc
      241 cgggcccatg tagcccatgt cgccttcctt cacttgcacg aagaagccgt tcgagccctt
      301 gccggtgacg agcgcgttct gcacgcgcac gacggagccg gtgttcacga tgctcgcctt
      361 gatctgatag atcgacgccg ggcagccggc ggtgccggga tttggatcgc tcgggcacgc
      421 gtcgcacgca tcgccgtggc cgtccatgtc gccatcggtc tggttcgggt tcgcgacgtc
      481 ggggcagttg tcggcggtgt tcgtgacacc gtcgtgatcg cgatcgttcg gatcggcgtt
      541 cgtgcacgtg gtcgagcccg cgtcgagcgg acagacgtcg cacgcgtcac cttggccatc
      601 gccgtcggcg tccggctgca tgccgttgtc catcggccgc accgggttga acgtgtcggg
      661 gcagttgtcg ctcgcgttcg ggatgccatc accgtcgttg tcgtcggctg acgtctgacc
      721 ggtgaacacc gtcgagccgt tgactgccat cggccgcttc ggcgagcacg agggctcctt
      781 ggccggcgtg ccgcacgcga acgcgggata gatcgtgccg accgcggtct tcagctcatc
      841 ccaggtcttg ccgatctcgg gcttcacgca cacgcgcttg tcgttgccgc acacgtcgac
      901 ttcgtcgcac tcgctgcgca gctcgttgac cgcggtcgca tcgccgtaga gcgccttgcc
      961 gccacgcatc acgagcgcga cgtccttcgg ctccgcttcg atgatcgcgc ggtacgcatc
     1021 cttgccgttg gcggcaaaga tcgcgatgtc cgcgacatga cctggcgcga gcatgccgat
     1081 cgcgtcatcg gtcgcggtga ccgcggcggc gttgagcgtc gccatcgccc acagctgctt
     1141 gtcggtgaag tagtggtcga agtacgtcgc gttgagctcg tcggcgcagt ggagctcgcg
     1201 cagcatgttc atcgagccgg ttggcatcca gtcggtaccg agcgcgatct cgacgccgag
     1261 ccgtgcagct tcggcgacgc gcgcggtgtc accgtagagc gtgatgttgg agcgcggcga
     1321 ccagatcagt gcgctgccgg cgactgccat cgcgccgtag tcgtttggcg tcaggccgat
     1381 cgcgtgaatc atcgccgtct tcggcaacag caagttctcc gacagaccgg gcgccatcgt
     1441 gtcgtaggta tcggagctct cgcacttgaa ctcgttgttt gcggcggcgt tgatgccttc
     1501 ggacgtgtgc ggctcgaacg aattgacgtt gctcagcgac tccggcgtca ccgtcgtgcc
     1561 gtagttgcag tccgcggtgc gcatcgtgcc ggacgagtcg ccgagcggga acgtctcgaa
     1621 gttgacggcc gtgtggttga ggccttcctg gttcgacgtg ttgtcgagat tgcggagcag
     1681 gccgggcgag ccgcccgagc cgacgatcga cgtcgcgcca cccatgacgt agcgcagctc
     1741 gccgtagctg atcttcacgc cactcgcgcc gccggtcgac ggaatcttcg tgtgaccgtc
     1801 gaggcccttg cgccactgat ggcggtgctc gtagcgctcg ccggtgtcgg tgtacggctc
     1861 ggcctgctgg aacgtgatgt gctcgtgcgt gttgatgagg ccgggcgata cggtcgcgcc
     1921 cgggcacacg atcgttgtct cgccaccctt cgcgcagtcg caaccgacgc acgtgatctc
     1981 gccggtcgca tcgatcgcga cctggccgcc gcggaagatc gtctgcggcg tgaggatgtt
     2041 gcccttgatc agctgcgccg cgccgaccga cgtgacctcg cacgttcctg acgcgagtgg
     2101 cggcaggttc tcgcagacga tttcctcggg accgccgtcg ggcgcatcca tgccgccatc
     2161 a
//
LOCUS       DASUFX010000340         4164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_53066,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000340 DASUFX010000000
VERSION     DASUFX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53066"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFV99_13625"
     CDS             complement(<1..213)
                     /locus_tag="VFV99_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060400.1"
                     /translation="MKSALSWLVHIAYGSLVAAAYGALRAGHPRNTFLDGIALGTGLW
                     LFGDELAVPLLGLADKPTAYSPSSHLQ"
     gene            504..1538
                     /locus_tag="VFV99_13630"
     CDS             504..1538
                     /locus_tag="VFV99_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5060401.1"
                     /translation="MRHVVVLAGGSGTRLWPASRQARPKQLIALTADGVPMVAAAVKL
                     GAAVAPNVVIVTGESHVEGTKAAVPGVEIIAEPVGRNTAAAIGLAAALLAARDPDAVM
                     LVLPADQHVTDKAAFAKALDAALVAVERDDSIATIGITPTRPETGFGYLEVDSAAPGL
                     VTAVKRFVEKPDRATADQYLASGRYLWNAGIFGASARRILAELDTHLPATARAVRDIA
                     RDPSTARTLYPTLASISFDHAVMEKADRVVTVPAQVGWDDVGSWAALPAVRGVDATGN
                     TVVGTALVIDGTGNVVVSDDTTLVATVGLSDLVVVKAGDAILVIRKDEAQDVRKVIDA
                     LSARGLARYL"
     gene            1542..2903
                     /locus_tag="VFV99_13635"
     CDS             1542..2903
                     /locus_tag="VFV99_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5060402.1"
                     /translation="MNPRSFREYDIRGVAERDLDDATVRALGTALGQRVTGQTVVVGR
                     DPRVHSPRLFAALTDGIRVHADVIDIGVVPTPVLYFAAHHLQPAASVMITGSHNPPED
                     NGFKLMMGSETLHGPAIAELRNEVESLVGKPVEPPHKSLQSRDVTGAYLDHAAGQLEL
                     GPRRFKVVVDAGNGAGGPTAVSLYRRLGFDVIPLYCELDGRFPNHHPDPTQPENVRDL
                     IATVQSNQAELGIALDGDADRIGAIDGTGRILWGDQLMILFGKALLAEVPHAKFVGEV
                     KCSQAMYDELAAAGGDVEMWKVGHSLIKARMKETGAQLAGEMSGHMFFAHRWLGFDDA
                     IYAGARLLELLSAGNRTLAERAAELPVMINTPEIRIDCPDDKKVALVAAVTARLRNDP
                     AVTGVVDVDGVRAKFAGGWGLVRASNTQPALVMRCEADTEQRLAEIRGAIEAHLDAAR
                     KSS"
     gene            2900..3856
                     /locus_tag="VFV99_13640"
     CDS             2900..3856
                     /locus_tag="VFV99_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5060403.1"
                     /translation="MKIGIDLGGTNLRAAIIEDEVRLRDHSKEPVGEPRDPTTIVARC
                     AELVERLSAGESATVGIGLAAMLRGHSGMVANSPHLRWLDIPFGELLAQRLGSRFKVG
                     VYNDVNAIVYGEYMCGSARGFRNVLGVYVGTGIGGGMIVNGELVEGASNCAGEIGHTK
                     VRWDDAAAPCACGKRGCVEAYVGGSYVQRRILEELSAGARSSAVTLAGGIENVNAGHV
                     DMAAADGDEWALGLWQELAPLLAVTLGNAMAVLNPERLVLGGGVLGRCPTLYAMTDAA
                     LMVVGNQPTLEACTIAQAELGDDAGIIGAAHLASRDVSIVAV"
     gene            3951..>4164
                     /locus_tag="VFV99_13645"
     CDS             3951..>4164
                     /locus_tag="VFV99_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060404.1"
                     /translation="MTRSPIDLTFTQLRDMDDALVRFCEALDEPKHGDPPIESLAREA
                     YLSVLAFAQLIYGVHGDDSAISTLALT"
BASE COUNT          671 a         1388 c         1420 g          685 t
ORIGIN      
        1 ctgcagatgg ctgctcggcg aatacgcggt cggtttgtcg gcaaggccga gcaacggcac
       61 cgcgagctcg tcgccgaaca gccacagccc cgttccgagc gcgataccgt cgaggaaggt
      121 gttgcgcgga tggcccgcgc gcagcgcgcc gtacgcggcc gcgacgagcg agccatacgc
      181 gatgtggacg agccaggaga gcgccgattt cattcttggc gacggcgagt gacccgcgag
      241 tttctggtac gcgagccgcc cgattgcggt ggtagcgctt tccccgggct cgtggtgcgg
      301 gccgaacggt gacatcgagc gcgcgcggag attcgcgtgc gtggcccgtg gtgcccgttg
      361 cgtcacgagc ggagcgctgg cacgtcggac gatctccatg agcccaagac cgatcgcgcc
      421 gccgagtagt ccaagaccga gtccttttcc tatcgcgtgc atggtgcgta gcgatgcaga
      481 cgccgttcca ctaggatgcg cccgtgcgtc acgttgttgt cctagccggt ggtagtggca
      541 cgcgcttatg gcccgcgagc cggcaggcgc ggcccaagca actgatcgcg ctcaccgcgg
      601 atggcgtgcc gatggtcgct gccgccgtga agctcggcgc cgccgtcgca ccgaacgtcg
      661 tgatcgtcac gggcgagagt cacgtcgagg gcacgaaggc tgcagtaccg ggcgtcgaga
      721 tcatcgccga gccggtcggt cgtaacacgg ccgctgcgat cggcctcgcc gctgcgctgc
      781 tggcggcgcg tgatcccgac gcggtgatgc tcgtcttgcc tgccgatcaa cacgtcacgg
      841 acaaggctgc attcgccaag gcgctcgatg ccgcactcgt cgccgtcgaa cgcgatgatt
      901 cgatcgcgac gatcggcatc acgccgacgc ggccggaaac gggctttggc tacctcgagg
      961 tcgacagcgc ggcgcccggc ctggtgaccg ccgtgaagcg cttcgtcgag aaaccggatc
     1021 gcgcgaccgc cgaccagtat ctcgcgagcg gtcgctatct gtggaacgcg ggcatcttcg
     1081 gcgcaagcgc gcgccgcatc ctcgcggagc tcgacacgca cttgcctgcg actgctcgcg
     1141 cggtgcgcga tatcgcacgc gacccgagca cggcgcgcac cctgtatccg acgctcgcat
     1201 cgatctcgtt cgatcacgcc gtcatggaga aggcggatcg cgtcgtcacc gtaccggcgc
     1261 aggttggctg ggacgacgtc ggctcgtggg ccgcgctgcc ggccgtccgc ggcgtcgatg
     1321 caacgggcaa taccgtggtt ggcactgcac ttgttatcga tgggacgggc aacgtcgtgg
     1381 tttccgacga cacgacgctc gtcgcgacgg tcgggctctc ggacctcgtc gtcgtcaagg
     1441 cgggcgacgc gatcctggtc atccggaaag acgaggccca ggatgttcgc aaggtcatcg
     1501 atgccctttc cgcacgcggc cttgcgcggt atctgtgact cgtgaacccg cgtagctttc
     1561 gcgagtacga catccgcggg gttgcggagc gcgacctcga tgacgcgacc gtgcgtgcgc
     1621 tcggcactgc actcggccag cgtgtcactg ggcagacggt tgtcgtcggc cgcgatccgc
     1681 gggtgcactc gccacggctg ttcgccgcgc tcaccgacgg cattcgcgtc cacgcggacg
     1741 tcatcgacat cggcgtcgtg ccgacgcccg ttctctactt cgcggcgcac catctccagc
     1801 cggccgcctc cgtgatgatc acgggaagtc acaacccgcc cgaggacaac ggcttcaagc
     1861 tgatgatggg cagcgagacg ttgcatgggc ccgcgatcgc cgagctgcgc aacgaggtcg
     1921 agtcgcttgt cggcaagccg gtcgagccgc cgcacaagtc gcttcaatcg cgcgacgtca
     1981 ccggtgcgta tctcgatcac gcggcaggtc agctcgagct cggcccgcgt cgcttcaagg
     2041 tcgtcgtcga tgccggcaac ggcgcgggcg ggccgaccgc cgtgtcgcta taccgacggc
     2101 tcggcttcga cgtcatcccg ctgtactgcg agctcgacgg tcgctttccg aatcatcacc
     2161 ccgatccgac gcagcccgag aacgtgcgcg atctgatcgc gacggtgcaa tcgaatcaag
     2221 ccgagctcgg cattgccctc gatggcgacg ccgatcgcat cggcgcgatc gacggcacgg
     2281 ggcgcatcct gtggggcgat cagctgatga tcctgttcgg caaagcgctg ctcgccgaag
     2341 tgccgcacgc gaagttcgtc ggcgaggtga agtgctcgca ggcgatgtac gacgagctgg
     2401 cggccgccgg cggtgacgtc gagatgtgga aggtcggcca ctcgctgatc aaggcgcgca
     2461 tgaaggagac cggcgcgcag ctcgccggcg aaatgtcggg ccacatgttc ttcgcgcatc
     2521 gctggctcgg cttcgacgac gcgatctatg ccggcgcccg gctgctcgag ctgctcagcg
     2581 cgggcaatcg cacgctcgcc gaacgggcgg ccgagctgcc ggtcatgatc aacacgcccg
     2641 aaatccgcat cgactgcccc gatgacaaga aggtcgcgct cgtcgcagcc gtgaccgcgc
     2701 gactgcgcaa cgatccggcg gtaaccggcg tcgtcgatgt cgatggcgtg cgcgcgaaat
     2761 tcgcgggcgg ctggggcctc gtgcgcgcgt cgaacaccca acccgcactc gtgatgcgct
     2821 gcgaagccga taccgagcag cggcttgccg agatccgagg cgcgatcgag gcgcacctcg
     2881 acgcggccag aaagtcctca tgaagatcgg catcgatctc ggcggcacga atctgcgcgc
     2941 ggcgatcatc gaggacgagg tcaggctgcg cgaccacagc aaggagccgg tcggcgagcc
     3001 gcgcgatccg actacgatcg tcgcgcgctg cgcggagctc gtcgagcggc tgtcagccgg
     3061 tgaatccgcg actgtcggca tcggcctcgc cgcgatgttg cgcggtcata gcggcatggt
     3121 tgcgaactca ccgcatctgc gctggctcga catcccgttc ggcgagttgc tcgcacaacg
     3181 cctcgggtcg cgcttcaagg tcggcgtcta caacgacgtc aatgcgatcg tgtacggcga
     3241 atacatgtgc ggctctgcgc gcggctttcg caacgtactc ggcgtctacg ttggcacggg
     3301 catcggcggc ggcatgatcg tcaacggcga gcttgtcgaa ggtgcgagca actgcgccgg
     3361 cgaaatcggc cacacgaaag tgcgatggga cgacgccgct gccccatgcg cgtgcggcaa
     3421 gcgcggttgc gtcgaagcgt acgtcggcgg cagctacgtc cagcgccgga tcctcgagga
     3481 gctgtcggca ggcgcgcgca gctcggcggt cacgctcgcc ggcggcatcg agaacgtcaa
     3541 cgctgggcac gtcgacatgg cggccgccga tggtgacgag tgggcgctcg gactctggca
     3601 agagctcgcg ccgctgctcg cggtcacgct cggtaatgcg atggcggtgc tcaatcccga
     3661 gcggctcgta ctcggcggcg gcgtgctcgg gcgctgccca acgctctacg cgatgacgga
     3721 tgctgcgctg atggtcgtcg gcaaccagcc aacgctcgag gcgtgcacga tcgcgcaagc
     3781 cgagctcggc gatgacgccg gcatcatcgg cgccgcgcac ctggcgtcgc gcgacgtttc
     3841 gatcgtggcc gtgtaacgga cgcgctgtcc gccgctcgtc ggacacggac acctcgcgcg
     3901 tgttggttca acctgttacg cgtggcgcgc gcaatgcagt gcgacgcagc atgactcgtt
     3961 cgcctatcga cctcacattc actcaacttc gcgacatgga cgatgcgctc gtgcgcttct
     4021 gtgaagcact cgacgagccg aagcacggcg atccgccgat cgaatcgctc gcacgcgagg
     4081 cgtatctgtc cgtgctcgcg ttcgcgcagc tgatctacgg cgtgcacggt gacgacagcg
     4141 caatatcgac cctcgcgctg actc
//
LOCUS       DASUFX010000341         6961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1303306, whole genome shotgun sequence.
ACCESSION   DASUFX010000341 DASUFX010000000
VERSION     DASUFX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303306"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VFV99_13650"
     CDS             <1..1289
                     /locus_tag="VFV99_13650"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5060405.1"
                     /translation="EACDDGNTTATDGCSATCTTESGWSCSGSPSSCSPVCGDGMVVG
                     TEACDDGGTTAGNGCSATCTVETGWTCSGAPSTCTAICGDGMVLGTETCDDGNTTASD
                     GCNATCMTESGWTCAGMPSTCMTTCGDGIIAGAETCDDNNSSAGDGCGATCLVEAGWT
                     CAGSPSTCSTTCGDGIKAGAEGCDDGGTTSNDGCSATCTVEGGWSCTGVPSVCTTSCG
                     DGVVAGTETCDDGNTAASDGCSATCTTETGWTCTGAPSACDEICGDGLVVGTEACDDG
                     DTDAGDGCNASCGVEGGWSCTGTPSVCTPNCGDGQVAGSESCDDNNNADGDGCSAACQ
                     TEAGWNCAGTPSVCTVVCGDGVVTASEECDDGNTTASDGCSATCVVEDGFECEAGSPS
                     KCTKLEDGCGCRSSGNTPPVLALLVLVALRRRRRRG"
     gene            complement(1286..2557)
                     /locus_tag="VFV99_13655"
     CDS             complement(1286..2557)
                     /locus_tag="VFV99_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060406.1"
                     /translation="MHSLGRSLILAAIAVIALRGSIARADDKPGGKGVEFIDDAKLLY
                     RIAACGGTDAIDDKLTKIVEKHCKAITEKLDKFRAEYFGKNREWFTAVVPKDVPKTVV
                     YPFGGGDLLSALVAFPDATEITTVSLELSGDPRRLKTLKPTAVQLSLGGLRGEIGGLI
                     SVGSNTTENLSAGQRNDLPAQVSSFLLGLVAGGYEPVSMRFFTLDDAGDIHYLEQSEI
                     DEMDKEAAKKLKKQKAAPPPKPVTPVVEGEPPPPVTAKTHNWLNPNFSLAFSNVEIQY
                     RKIGETAVRVHRHIGWNLSDGYLKTHPQLIRHLEKKGQVTMLVKGASYLLWSPGFSRV
                     RGYILDHLAWMLSDSTGVPPAFAKPAGMVQETYGIYTGAFLESWQATKHDEDFVALWK
                     GQKKRRLPFRFGYVDMTKQAHLVVTRPRAKS"
     gene            complement(2595..4448)
                     /locus_tag="VFV99_13660"
     CDS             complement(2595..4448)
                     /locus_tag="VFV99_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5060407.1"
                     /translation="MAESVAPEKMTRNTTSLPQLMMQRVQQTPDREAYRYPVGEAWRS
                     MTWRQAGERVRAIAAGLLTLGLHREERVGILSNTRVEWLLCDLGILCAGGATTTVYPS
                     STPEDCAFILSDSETRYIFVEDAGQVAKLRNHRAEMPKLTKLILLDGVPDAKDGDWVI
                     TLADLEAKGAELLAKDPRAIDDIVKGVEGKQLATLIYTSGTTGKPKGVRLLHECWAYT
                     ADAIDAIKLWGPDDVQYLWLPMSHSFGKVLMAGHIASGSVTAVDGRIPKIIENLGVVR
                     PTIMAAVPRIFEKVYNKILEGVKAGSPLKQQIFQWAVNVGKEGSKVRQRGEHPSGLLA
                     LKLKIADRLVFQKIKDRFGGRVHYFISGSAPLSREIAEFFHACGILILEGYGLTESSA
                     ASFVNRSSKYAFGSVGMPMPGTELKIAPEDGEILIRSPGVMQGYHNLPDATAETLTPD
                     GWLKTGDIGEVDQRGFLRITDRKKDLIKTSGGKYIAPQSIEGKLKATCPYISQVIVHG
                     DKRNFVTALVTLDEEATMKWAKEHGFNGQGYTEVVQAPEVKKLLDPYFAEVNKTLAKY
                     ESIKQFAILPKDLSVDEGELTPSLKVKRKVVEKKYATLLDKMYEGSVADMG"
     gene            4508..6481
                     /locus_tag="VFV99_13665"
     CDS             4508..6481
                     /locus_tag="VFV99_13665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IGHMBP2 family helicase"
                     /protein_id="HEX5060408.1"
                     /translation="MVSATEWLDSLSAAWRAERLAARARVALERSGRSLTERVALGIA
                     IAHLRIVDEQSAPGERVRVRVTVPEQVDLDNLRLAPGDPIRLWAEHPDESSAVRGVFE
                     RREEQSVWLMLERPVDELDRDYALDPEAPELTFDRGDAAIERARSALANSDLARLREV
                     ASLARPAKLLAPVTWTPLDEELDEKQRAAVDAGIRSGDLALIWGPPGTGKTRTLVEVV
                     RQRVARGERVLCTAPSNTAVDNLGIRLAEAGVRAVRLGHPARVSPALATLTIDAQIDR
                     DGTYDLVREWRDRAREIRRRAMSSDVTKGSREDIARARAEVRALWAEARALDRDAARE
                     IANAERAIVDRADVVLCTCVGCDHPILGNTVFDCVVVDEATQAPDPLLLIAFARAKVG
                     VLAGDPQQLGPVVIGGPSVDAVLGTTVFERLVGAGAPAVMLEQQHRMHEMIMTFPSVS
                     TYDGKLRAAPAVATHTLDDLGVKADTVRNRPVWLVDTAGKDWLEERTDFDPGGSLNNM
                     PSFSFDPSTFNSGNAERVAAEARRLLSRGLLPTDLAIIAAYSAQARRLRDLLRAERAA
                     GVEVGTVDGFQGREKEAVIVDLVRSNDSGELGFLSNTRRMNVALTRARRFLLVIADSA
                     TLGEHPYYARFLQYVDEIDAHGSAWSDDAEPLP"
     gene            6514..>6961
                     /locus_tag="VFV99_13670"
     CDS             6514..>6961
                     /locus_tag="VFV99_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060409.1"
                     /translation="MKAILFVAALALAAACGKHNDAAILQQEAITLAKHYQAKPDKPS
                     QLDLLDQRVQAIFARGNKIPGNLPGIEEVGKRLQEARDTIVKLNGIVGKGPDGKSALE
                     KQAEAAAKEGKVADLQKLVHDSEEELERGLTIINDDLDAVEAWLANY"
BASE COUNT         1178 a         2330 c         2275 g         1178 t
ORIGIN      
        1 cggaagcctg cgacgacggc aacacgaccg cgaccgatgg ctgtagcgcg acgtgcacca
       61 ccgaatccgg ctggagttgc agcggatcgc cgagctcgtg ctcgccggtc tgcggtgacg
      121 gcatggtcgt tggcaccgaa gcctgcgacg acggcggcac gacggcgggc aacggctgct
      181 cggccacgtg tacggtcgag acaggctgga cgtgcagcgg cgcacccagc acgtgcacgg
      241 cgatctgtgg cgatggcatg gtgctcggca ccgagacgtg cgacgacggc aacacgaccg
      301 ccagcgacgg ctgcaacgcg acgtgcatga ccgagtcggg ctggacgtgc gcgggcatgc
      361 cgagcacgtg catgacgacc tgcggtgacg gcatcatcgc cggtgccgaa acctgcgacg
      421 acaacaacag cagtgcgggc gacggctgcg gcgcgacctg cctcgtcgaa gcgggatgga
      481 cctgcgcggg ctcaccgagc acgtgcagca cgacctgtgg cgatggcatc aaggccggcg
      541 ccgagggctg cgacgacggc ggtacgacga gcaatgacgg ctgctccgcg acgtgcaccg
      601 tcgagggtgg gtggtcgtgc accggcgtgc cgagcgtgtg cacgacgtcg tgcggcgacg
      661 gtgttgtcgc aggcaccgag acctgcgacg acggcaacac agccgcgagc gacggctgca
      721 gcgcgacgtg caccacggaa acgggctgga cgtgcaccgg cgcgccgagc gcctgcgacg
      781 agatctgcgg tgatggtctc gtcgtcggca ccgaggcgtg cgacgacggt gacaccgatg
      841 caggtgacgg ctgcaacgcg tcgtgcggtg tcgaaggtgg ctggtcgtgc accggcacgc
      901 cgagcgtctg cacgccgaac tgcggcgatg gccaggtcgc gggctccgag tcctgtgacg
      961 acaacaacaa cgcggacggc gatggttgct cggctgcgtg ccagaccgaa gcgggctgga
     1021 actgcgccgg aacgccgagc gtgtgcacgg tcgtgtgcgg cgacggtgtt gtcaccgcga
     1081 gcgaggaatg cgacgacggc aacacgaccg cgagcgacgg ctgcagcgcg acgtgcgtcg
     1141 tcgaggatgg cttcgaatgc gaggccggct cgccgagcaa gtgcaccaag ctcgaggacg
     1201 gctgcggttg ccgtagcagc ggcaacacac cgccggttct ggcgctcctc gtcctcgtcg
     1261 cgctccgccg ccgccgccgc cgcggttagc tcttggcgcg gggacgcgtg acgacgaggt
     1321 gcgcctgctt cgtcatgtcg acgtagccga agcggaacgg cagccggcgc ttcttctggc
     1381 ccttccagag cgcgacgaag tcttcgtcgt gtttcgtcgc ctgccagctc tcgaggaacg
     1441 caccggtgta gatgccgtat gtctcttgca ccatgcccgc cggcttcgca aacgcgggtg
     1501 gcacgccggt cgagtccgac agcatccacg cgaggtgatc gaggatgtaa ccgcgcacgc
     1561 gcgagaagcc gggactccac agcaagtagc tcgcgccttt gacgagcatc gtgacctgtc
     1621 ccttcttctc gaggtgacgg atcagctgcg ggtgggtctt gagatagccg tcgctgaggt
     1681 tccacccgat gtgacgatga acgcgcaccg cggtctcgcc gatcttgcgg tactggatct
     1741 cgacgttcga gaacgcgagc gaaaagttcg ggttcagcca gttgtgcgtc ttcgcggtga
     1801 ccggcggcgg cggttcgccc tcgacgactg gcgtcactgg cttcggcggc ggcgccgcct
     1861 tctgcttctt gagcttcttc gcggcttcct tgtccatctc gtcgatctcg gactgctcga
     1921 gataatggat gtcgcccgca tcatcgagcg tgaagaagcg catcgacacc ggctcgtacc
     1981 cgccggcgac gaggccgagc aagaacgagc tgacttgcgc cggcaggtcg ttgcgctgcc
     2041 ccgccgacag attctccgtc gtgttcgagc cgaccgagat caagccaccg atctcgccgc
     2101 gcagaccacc aagactgagc tgcaccgcgg tcggcttgag cgtcttgagg cggcgcggat
     2161 caccggagag ctcgagcgag accgtcgtga tctcggtcgc gtccggaaac gcgacgagcg
     2221 ccgacaatag atcgccgccg ccgaacggat agacgaccgt cttcggcacg tccttcggaa
     2281 cgacggcggt gaaccactcg cggttcttgc cgaagtattc ggcgcggaac ttgtcgagct
     2341 tctcggtgat cgccttgcag tgcttctcga cgatcttcgt gagcttgtcg tcgatcgcgt
     2401 cggtgccacc acacgcggcg atgcggtaca gcagcttcgc gtcatcgatg aactcgacgc
     2461 ccttgccacc gggcttgtcg tccgcccgcg cgatcgatcc tcgaagtgcg atgaccgcga
     2521 tagccgcgag gatcaggctc cgccccagac tatgcattgt cagatcgaac gcgcgagaac
     2581 gctccaccct tccactatcc catatccgcc acgctgccct cgtacatctt gtcgagtagc
     2641 gtcgcgtact tcttctcgac gaccttgcgc ttcaccttga ggctcggtgt cagctcgcct
     2701 tcatccaccg acaagtcctt cggcaggatc gcgaactgct tgatgctctc gtacttcgcg
     2761 agcgtcttgt tcacctcggc aaaatacgga tcgagcagct tctttacttc gggggcctgc
     2821 acgacctcgg tatagccctg gccgttgaac ccgtgctcct tcgcccactt catcgtcgcc
     2881 tcttcgtcga gcgtgacgag tgcggtgacg aagttgcgct tgtcgccgtg gacgatcacc
     2941 tggctgatgt acgggcacgt cgccttcagc ttgccctcga tcgattgcgg cgcgatgtac
     3001 ttgccgccgc tggtcttgat cagatccttc ttgcgatccg taatgcgaag gaagccgcgc
     3061 tgatcgactt cgccgatgtc gccggtcttg agccagccat cgggcgtgag cgtctcggcc
     3121 gtcgcgtcgg gaaggttgtg atagccctgc atgacaccgg gcgagcgaat gagaatctcg
     3181 ccgtcctcgg gcgcgatctt gagctcggtg ccgggcatcg gcatgccgac gctgccgaac
     3241 gcgtacttgc tcgagcgatt gacgaagctc gcggcgctcg attccgtgag gccgtagccc
     3301 tcgagaatca ggatgccgca cgcgtgaaag aactccgcga tctcgcgcga cagcggtgcc
     3361 gagcccgaga tgaagtagtg cacgcgaccg ccgaaccgat ccttgatttt ctggaacacg
     3421 aggcggtctg cgatcttgag cttgagcgcc agcaagccgg atgggtgctc gccgcgctgc
     3481 cgcaccttcg agccttcctt tccgacgttg accgcccact ggaagatttg ctgcttgagc
     3541 gggctgcccg ctttcacgcc ctcgaggatc ttgttgtaga ccttttcgaa gatgcgcggc
     3601 acggccgcca tgatcgtcgg ccgcacgacg ccgaggttct cgatgatctt cggaatgcgg
     3661 ccgtcgacgg cggtcaccga gcccgacgcg atgtggccgg ccatcagcac cttgccgaac
     3721 gagtgagaca tcggcagcca caggtattgc acgtcgtcgg ggccccacag cttgatcgcg
     3781 tcgatcgcat ccgctgtgta cgcccagcac tcgtggagta ggcgcacgcc cttcggcttg
     3841 ccggtggtgc ccgacgtgta gatcagcgtc gcgagctgct tgccctcgac gcctttgacg
     3901 atgtcatcga tggcgcgcgg atccttcgcg agcagctcgg cgcccttcgc ttcgagatcg
     3961 gccagcgtga tcacccaatc gccgtccttc gcgtcgggca caccgtcgag caagatgagc
     4021 ttcgtgagct tcggcatctc cgccctgtga ttgcgtagct tcgcaacctg gccggcgtcc
     4081 tcgacgaaga tgtagcgagt ctcggagtcc gacaggatga acgcgcagtc ctccggcgtg
     4141 ctcgatgggt agacggtcgt cgttgcgccg ccggcacaca agataccgag gtcgcagagc
     4201 agccactcca cgcgcgtatt cgacagtatg ccgacgcgct cctcgcgatg aagaccgagc
     4261 gttaacaggc ccgccgcgat cgcacgtacg cgctcgccgg cctggcgcca ggtcatcgac
     4321 cgccacgcct caccgaccgg gtagcgatac gcctcgcggt ccggcgtctg ttgcacgcgc
     4381 tgcatcatca gctgcggcaa cgacgtggtg ttgcgggtca tcttctccgg ggcaaccgat
     4441 tccgccataa gcgggctgac tatgccccaa ttcctctcgc tgtggatcgg agttacgctc
     4501 ccccaaaatg gtgtccgcaa ccgaatggct cgactcgctc agcgccgcct ggcgcgccga
     4561 gcgacttgcc gcacgcgcgc gtgtcgcgct cgaacgcagc ggacggtccc tcaccgaacg
     4621 agtagcgctc ggtattgcga tcgcacacct acgcatcgtc gacgagcaat cagcgcccgg
     4681 cgagcgcgtg cgcgtgaggg tgacggtccc cgagcaagtg gatctcgaca acttgcgact
     4741 cgcgcccggc gatccgatcc ggctgtgggc cgagcatccg gacgaatcga gcgcggttcg
     4801 cggcgtgttc gagcgccgcg aggaacagag cgtgtggctg atgctcgagc gtccggtcga
     4861 cgagctcgat cgcgactacg cactcgatcc cgaggcaccg gagctgacat tcgatcgcgg
     4921 cgatgcagcg atcgagcgcg cgcgttccgc cctcgcgaac tccgatctcg cgcgcctgcg
     4981 tgaagtcgcg tcgcttgcac gtcccgccaa gctgctcgcg cctgtgacct ggacgccact
     5041 cgacgaggag ctcgacgaga agcaacgcgc cgcagtcgac gccggcattc gcagcggtga
     5101 tctcgcattg atctggggcc cgccgggtac cggcaagacc cgcacgctcg tcgaggtcgt
     5161 gcgccaacgc gtcgcgcgcg gtgagcgcgt gctgtgcacg gcaccgtcga acacggccgt
     5221 cgacaacctc ggcatccggc tcgccgaggc tggtgtacgc gccgtacgac tcggccatcc
     5281 agcgcgtgta tcaccggcgc tcgcgacact gacgatcgac gcgcagatcg accgagacgg
     5341 cacctacgac ctcgttcgcg aatggcgcga tcgtgcgcgc gagattcgcc ggcgtgcgat
     5401 gagcagcgac gtcaccaagg gcagccgcga ggacatcgcg cgggcgcgtg ccgaggtacg
     5461 tgcgctatgg gccgaagcgc gtgcgctcga tcgcgatgct gcacgcgaaa tcgcgaacgc
     5521 cgagcgcgcg atcgtcgacc gcgccgacgt ggtgctgtgc acgtgtgtcg gctgcgatca
     5581 tccgatcctc ggcaacacgg tcttcgattg cgtcgtcgtc gatgaagcga cacaggctcc
     5641 cgatccgctg ctcctcatcg cgttcgcgcg cgcgaaggtc ggcgtgctcg ccggcgatcc
     5701 gcagcagctc ggtccggtcg tgatcggcgg gccctccgtc gacgccgtgc tcggcacgac
     5761 cgtgttcgag cggctcgtcg gagcgggcgc gcccgcggtg atgctcgagc agcagcatcg
     5821 catgcacgag atgatcatga cgttcccgtc ggtctcgacg tacgacggca agctgcgcgc
     5881 cgcaccggcg gtcgcaacgc acacgctcga cgacctgggc gtcaaagccg acaccgtgcg
     5941 caatcgaccg gtctggctcg tcgacaccgc cggcaaggac tggctcgagg agcgcaccga
     6001 tttcgatccc ggtggctcgc tcaacaacat gccgtcgttc agcttcgatc cgagcacgtt
     6061 caactccggc aacgccgagc gcgtcgccgc ggaggctcgc cgactgctgt cgcgcggcct
     6121 gttgccgacc gacctcgcga tcatcgccgc ctactcggcg caggcccgcc ggctccgcga
     6181 cctgctgcgc gccgagcgtg ccgccggtgt cgaggtcggt accgtcgacg gcttccaagg
     6241 gcgcgagaag gaagccgtga tcgtcgatct cgtgcgcagt aacgactccg gcgagctcgg
     6301 cttcctctcg aacacccggc gcatgaacgt cgcgctcacc cgtgcgcgac ggttcttgct
     6361 cgtgatcgcg gactccgcga ccctcggcga acacccgtac tacgcgcggt tcctgcagta
     6421 cgtcgatgaa atcgatgccc acggcagcgc gtggagcgac gacgccgagc ccttgccata
     6481 gccccgctga atctgcgatc aacagggggt cgtatgaagg caatcctctt tgtcgccgcc
     6541 ctcgccctcg ctgccgcttg cggcaagcac aacgatgccg ccatcctcca gcaggaggcc
     6601 atcacgctcg cgaagcacta tcaggccaag cccgacaaac cgtcccagct cgacctgctc
     6661 gatcagcgcg tgcaggcgat cttcgcgcgc ggaaacaaga tcccagggaa ccttcccggc
     6721 atcgaggagg tcggcaagcg cctccaagaa gcccgcgaca ccatcgtgaa gctgaacggc
     6781 atcgtcggca aaggtcccga cggcaagtca gcgctcgaga agcaggcaga agccgccgcg
     6841 aaggaaggca aggtcgctga tctgcaaaag ctcgttcacg acagcgagga agagctcgaa
     6901 cgcggcctga cgatcatcaa cgacgatctc gatgccgtcg aagcgtggct cgccaactac
     6961 g
//
LOCUS       DASUFX010000342         8250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_471249,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000342 DASUFX010000000
VERSION     DASUFX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471249"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFV99_13675"
     CDS             complement(<1..599)
                     /locus_tag="VFV99_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060410.1"
                     /translation="MTYARFVGLALLVAATGCDDDDDQCQFYDRGGAPGIAAIEYRDP
                     VSGTCQAFGGGYDCADPCQPCPDYATGAAAQPDWAQCYQQCEGLDENTCKGTPSCRAI
                     YKGNNTVFHECWGVAQSGPVQGGNCVAFDAYECSRHDDCIAIHADGSPIGSFSSCAPE
                     TSDPNPGSCVGAITCTTAAPACPSGTLAGRANGCWTGYC"
     gene            complement(685..1650)
                     /locus_tag="VFV99_13680"
     CDS             complement(685..1650)
                     /locus_tag="VFV99_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060411.1"
                     /translation="MRFGCAPLVVLVTACYGPSVNPGSPCGPNGECPSGLECISGLCL
                     PSGSTPHDDAAMPDDSAVEIDGNIVLADAAPDGALPYVPWGTPVAVPSLDAVSGHGEG
                     DPSITANKLTVLLTSDSPDDIFECNRTAIGQAFTCFVLAINSVDDDKSPEVSADGTTL
                     YFSSDRGGSFDIYISKKTMGVWSTPVVATDLSSATTDSDLAISPDGLTAVVEQDPGTN
                     HFVFFTRASTAVPFGSPVTHTELEGPIDIAAPSLTNNGDILYFHAGNTRDIYVATRKP
                     NGQYNMPKPVTELNTSSRDAAPFVSADDKYMLFERAGDIYETTRP"
     gene            complement(1672..3087)
                     /locus_tag="VFV99_13685"
     CDS             complement(1672..3087)
                     /locus_tag="VFV99_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX5060412.1"
                     /translation="MGKPTGFIEWPRAAPHRRKVDERLRDWREVETTADPAESKQQGG
                     RCMDCGVPFCTQGCPLGNPIPDFAHAVYTDKWESAHRQLAVTNEFPEFTGRLCPAPCE
                     SACVLAIDNAPVTIEALERAISDRAFKEGWIQPRPPKTRTGKRVAIVGSGPAGLAAAA
                     HINRFGHTAIVYEAAKRPGGLLRYGIPDFKMEKHVIDRRLEILSKEGVELRCGVAVGS
                     RSGHGPTWKQLRADHDAVVIAIGAQRPRELDVPGRNLDGVVLAMDYLTEQNEVVGGER
                     ESTRFDVKGKRVIILGGGDTGSDCLGTAHRQGAAHVMQIELMPAPPATRATTNPWPTW
                     AFVFRTSSSQEEGGVREFAFRTTRLEGKDGKLVALYGVKVEIKDGKLVDVPDSELKLE
                     VDTLILALGFVGPSVDPLVEQLGIALDSRKNVIVDKQFSTNVKGVYCAGDAHRGASLI
                     VWAIAEGRDMAKAVDIFLRGG"
     gene            complement(3080..7546)
                     /gene="gltB"
                     /locus_tag="VFV99_13690"
     CDS             complement(3080..7546)
                     /gene="gltB"
                     /locus_tag="VFV99_13690"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX5060413.1"
                     /translation="MDFTAGERDACGVGFVAHIKGERSRAIVDQGLEVLKRLSHRAAC
                     GADPDTGDGAGILMQLPEKFFRAEGARLGFDIPANRRFAIGNVFLPPDPGLRAACEGL
                     LESAIRDEGQQVIGWRDVPIDLRHVGRIARDVLPVFRQIYVRMRRVPPSAWERTLYVI
                     RKLTENRVRERRADPSGYFHVASLSTETIVYKGLLLPAQVPQFFTDLKSPLMESAIAV
                     VHSRFSTNTFPTWDLAQPFRYIAHNGEINTLRGNTNWMQARKSQLKSAKFSGGLERLW
                     PIIVPGKSDSAQFDNMVELLTLGGRTLPHSMMMMIPEAWEGNAAMDDDRKSFYRYASS
                     LLEPWDGPAAIVFSDGQLVGATLDRNGLRPARWTITTDDRVILASETGVIDVPPEQVR
                     QKGRLTPGMMFVVDTAEGRIVDDAELKRDVAGRFPYRKWLDKNVYELDELDAAEAPPS
                     ITGAPLLEQQRAFGYTDEDIDLIITPMADTGKEPVGSMGTDTPLAVLSQRAPSLFTYF
                     HQLFAQVTNPPIDPIRESLVMSLESSLGPDGNTFEESPEQCHQLALRSPILDSKDLAK
                     IRATQEGVFEPITLSLLWPVSHGAGWLEKALEKLCRAAVQAIDDGHNVLILSDRGCDK
                     QRVAIPSLLALSAVQQHLVREGIRMQVGLVVETAEVREVHDVALLIGYGAAAINPYLA
                     IDTVRAMGKEPKLYIKALEEGLLKVMSKMGISTVHSYRGSQIIEAVGLDRDLIDQWFT
                     GTPSRINGVGLEELGDEAVARHARGYMKTDDQLPIGGQYRWRRTGELHKWTPATIAAL
                     QDSVRTNDAAKFQEFERLCDDEENALVTLRGLLDIEGGDPIPLDEVEPIKSIVSRFVT
                     GAMSFGSISAEAHETLAIAMNRMGAKSNSGEGGEEPHRFVPDENGDFRRSAIKQIASG
                     RFGVTTHYLVNADDLQIKVAQGAKPGEGGQLPGNKIDDRIAKARNSTPGVTLISPPPH
                     HDIYSIEDLAQLIYDLTAVNPRARVSVKLVSEVGVGTIAAGVAKARAACVVIAGYEGG
                     TGASPLSSLKRAGLPWELGLAETQQVLVENRLRDRIRIQVDGGLRTSRDIVVAALLGA
                     EEFGMATASLIATGCVMLRKCHLNTCSVGIATQDPELRKKFVGKPEEVVAYFTFVAEG
                     VRRRLAEIGARSLDEVIGKVEYLKVRKRTLKKFDKTKDLDLSAIVRRPISHTKLPLRF
                     EKAQPWPLEDHLDHELIKRGALDGKPLVIELPIDNSKRAAGTLLSGELAKRYGSRGLP
                     DDTIHVKLTGHAGQSFGAFLSPGVTLELSGDANDYIGKGLSGGRLIVKPPVGSRFAPE
                     DNVIIGNVALYGATAGDLFANGLGGERFCVRNSGARAVVEGVGDHGCEYMTGGVVVVL
                     GPVGRNFAAGMSGGNAFVFDPAQSFRSRCNMDMVDLEPLAEESDLWLVHSLIEDHVRF
                     TGSPRGKKILDNWDHLIARFVKVMPIEYKRVLERRRSTTRPRSLMFAGAANG"
     gene            complement(7617..>8250)
                     /locus_tag="VFV99_13695"
     CDS             complement(7617..>8250)
                     /locus_tag="VFV99_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060414.1"
                     /translation="HLVMVVFSYNDVPFEQLTHGFAGMMLAPDEGQYFDPRSVMQRFD
                     LRGRSVINVPLTIDLAERRLRWLDVGIRGRRELHDVGGYRAALAHVGRDFADALGVGA
                     RPTLWDLACIHAAARGNVIYIRERDGSFTMYRRRDNESKAMRLARLMSGAADDGRAGT
                     LPATDAPTWFALVTGMPLPKGSVGYALDARELGPEVQQLSPGDLVAELAL"
BASE COUNT         1338 a         2710 c         2768 g         1434 t
ORIGIN      
        1 atgcagtagc cggtccagca gccgttcgcg cggcccgcga gcgtcccgga cgggcaggcg
       61 ggtgccgccg ttgtgcacgt gatcgcaccg acgcacgagc cgggattcgg atcgctcgtc
      121 tcgggcgcgc atgacgaaaa cgatccgatg ggcgaaccgt cggcgtggat cgcgatgcag
      181 tcgtcgtgac gcgagcactc gtaggcatcg aatgcgacgc agttgccgcc ttgcaccggg
      241 ccggactgcg cgacgcccca gcactcgtgg aacacggtgt tgttgccctt gtagatcgcg
      301 cggcacgacg gcgtgccctt gcacgtgttc tcgtcgagac cttcgcactg ctggtagcac
      361 tgcgcccagt caggctgcgc cgccgcgccc gtcgcgtaat cggggcacgg ctgacacggg
      421 tcggcgcagt cgtagccgcc gccgaacgcc tggcaggtgc cgctgacggg gtcgcgatac
      481 tcgatcgcgg cgatgcccgg agcgccaccg cggtcgtaga actgacactg atcgtcgtcg
      541 tcgtcgcagc cagtagccgc gacgagcagg gcaaggccaa cgaagcgcgc gtaggtcata
      601 gggacctaat cagcaaccat cgcgccagat gcaactgcgc gtgatgccat gtacgtggca
      661 cgtcgccgcc tcagatttcc gaggttatgg gcgcgtggtc tcgtagatat cgcccgcccg
      721 ctcgaacagc atgtacttgt cgtcggccga cacgaacggc gcagcgtcgc gtgacgaggt
      781 gttcagctcg gtgacgggct tcggcatgtt gtactggccg ttcggcttgc gcgtagcgac
      841 gtaaatgtcg cgcgtgttgc ccgcatggaa gtagaggatg tcgccgttgt tcgtgagcga
      901 cggcgccgcg atgtcgatcg gaccctcgag ctcggtatgc gtgaccgggc ttccgaacgg
      961 caccgccgtg gacgcgcgcg tgaagaacac gaagtgattg gtgccgggat cctgctcgac
     1021 gaccgcggtg aggccgtcgg gcgaaatcgc gaggtcggag tccgtggtcg cgctcgagag
     1081 atccgtcgcg acgaccggcg tcgaccagac gcccatggtc ttcttcgaga tgtagatgtc
     1141 gaacgacccg ccgcgatcag acgagaagta gagcgtggtg ccgtcggcgc tgacctctgg
     1201 tgacttgtcg tcgtcgaccg aattgatcgc gaggacgaag cacgtgaacg cctggccgat
     1261 tgcagtgcgg ttgcattcga agatgtcgtc cggactgtcc gacgtcagca ggacggtgag
     1321 cttgttcgcc gtgatcgacg gatcgccttc gccgtgcccg gacacggcat cgaggctcgg
     1381 taccgcgacc ggtgtgcccc atggcacata cggcagtgcg ccatccggcg ccgcgtcggc
     1441 gaggacgatg ttgccgtcga tctcgactgc gctgtcgtcg ggcatcgctg cgtcgtcgtg
     1501 cggcgtgctg ccggacggca ggcatagccc gctgatacac tccagcccgc tcgggcattc
     1561 gccattcggc ccgcacggcg agcccggatt tacggatggc ccgtaacacg ccgtgacaag
     1621 cacgacgagc ggagcgcatc cgaaccgcat gcgtggatga taacgccgcg ctcagccgcc
     1681 gcggagaaat atgtcgaccg ccttcgccat gtcgcggcct tccgcgatcg cccacacgat
     1741 gagcgacgcc ccgcggtggg catctcctgc acagtagacg ccttttacgt tcgtcgagaa
     1801 ctgcttgtcg acgatgacgt tcttgcgaga gtcgagcgcg atgccgagct gctcgacgag
     1861 cggatcgaca ctcgggccca cgaatccgag cgcgaggatc agcgtgtcga cctcgagctt
     1921 gagctcgctg tccggcacgt cgacgagctt gccgtccttg atctcgacct tcacgccgta
     1981 cagcgcgacg agcttgccgt ccttgccctc gagtcgcgtg gtgcggaacg cgaactcgcg
     2041 cacgccacct tcttcttgcg agctcgacgt gcggaacacg aatgcccagg tcggccacgg
     2101 gtttgtcgtg gcacgggttg ctggcggcgc aggcatcagc tcgatctgca tcacgtgggc
     2161 agcgccctgg cgatgtgccg tgccgaggca gtcggatccg gtgtcgccgc cgccgaggat
     2221 gatcacgcgc ttgcctttga cgtcgaagcg cgtgctctcg cgctcgccgc caacgacctc
     2281 gttctgctcg gtgaggtagt ccatcgcgag cacgacaccg tcgaggttcc ggccgggcac
     2341 gtcgagctcg cgcggccgct gcgcaccgat cgcgatcacg accgcatcgt ggtccgcacg
     2401 cagctgcttc caggtgggcc catgaccaga tcgacttccg accgcgacgc cgcagcggag
     2461 ctcgacgcct tccttcgaga ggatctcgag gcggcgatcg atgacgtgct tctccatctt
     2521 gaagtcgggg atgccgtagc ggagcaggcc acccgggcgc ttcgccgctt cgtagacgat
     2581 cgcggtgtgg ccgaagcggt tgatgtgcgc cgcagccgcg agtcccgccg gtcccgagcc
     2641 gacgatcgcg acgcgcttac ccgtgcgcgt cttcggtggc cgcggttgga tccagccctc
     2701 cttgaacgcg cggtcgctga tcgcgcgctc gagcgcttcg atggtgacgg gtgcgttgtc
     2761 gatcgcgagc acgcacgccg actcgcacgg agcagggcag aggcggccgg tgaactcggg
     2821 gaactcgttc gtcaccgcga gctggcgatg cgcggattcc catttgtcgg tgtagaccgc
     2881 gtgcgcgaag tcgggaatcg gattgccgag cggacagccc tgcgtgcaga acggcacgcc
     2941 gcaatccata cagcgcccgc cttgctgctt gctctcggcg ggatccgcgg tggtctcgac
     3001 ctcgcgccag tcgcgcaagc gctcgtcgac cttccgccgg tgcggagccg cacgcggcca
     3061 ctcgatgaat ccagttggct tacccattgg cggctcctgc gaacatcagc gagcgcggac
     3121 gcgtcgtcga gcgacggcgt tcgagcacgc gcttgtattc gatcggcatc accttcacga
     3181 agcgcgcgat gaggtgatcc cagttgtcga ggatcttctt gccgcgcggg ctgccggtga
     3241 accggacgtg atcctcgatg aggctgtgga cgagccagag gtcgctctcc tcggcgagtg
     3301 gctcgagatc gaccatgtcc atgttgcagc gcgagcggaa cgactgcgcc gggtcgaaca
     3361 cgaacgcgtt accgccgctc atgccggccg cgaagttgcg gccgaccgga ccgagcacga
     3421 cgacaacgcc accggtcatg tactcgcaac cgtgatcgcc gacaccttcg acgaccgcac
     3481 gcgcgcccga gttgcgaacg cagaagcgtt cgccgcccag gccgttcgcg aacagatcgc
     3541 cggcggtggc gccgtacagc gcgacgttgc cgatgatcac gttgtcctcg ggcgcgaacc
     3601 gcgagccgac cggtggcttc acgatcaagc ggccacccga gaggcccttg ccgatgtagt
     3661 cgttcgcgtc gcccgacagc tcgagcgtga cgcccggcga caagaacgcg ccgaagctct
     3721 ggcccgcgtg gccggtgagc ttgacgtgaa tggtgtcgtc cggaagaccg cgcgagccgt
     3781 accgtttcgc gagctcgccc gatagcagcg tgccggctgc gcgcttgctg ttgtcgatcg
     3841 gtagctcgat cacgagcggc ttgccgtcga gcgcgccgcg cttgatcagc tcgtgatcga
     3901 gatgatcctc gagcggccac ggctgcgcct tctcgaaccg gagcggcagc ttcgtgtgcg
     3961 agatcgggcg gcgcacgatc gcggacagat cgaggtcctt cgtcttgtcg aacttcttga
     4021 gcgtgcgctt gcggaccttc aggtactcga ccttgccgat cacctcgtcg agcgagcgcg
     4081 caccgatttc ggcgagccgg cggcgcacgc cttccgcgac gaacgtgaag tacgcgacga
     4141 cttcttcggg cttgccgaca aacttcttgc gcagctcggg atcctgcgtc gcgatgccga
     4201 ccgagcacgt gttgaggtgg cacttgcgca gcatcacgca gccggtcgcg atcagcgacg
     4261 cggttgccat cccgaactcc tcggcgccga gcagcgcggc gacgacgatg tcgcgcgacg
     4321 tgcgcaggcc accgtcgacc tggatgcgga tgcgatcgcg cagccggttc tcgacgagca
     4381 cctgctgggt ctcggcgagg ccgagctccc acggcaagcc cgcgcgcttg agcgacgaca
     4441 gcggcgacgc accggtgccg ccttcgtagc cggcgatcac gacacacgct gcgcgtgcct
     4501 tcgcgacacc cgcggcgatc gtgccgacac cgacctcgct gaccagcttc acgctgacgc
     4561 gcgccctcgg attgacggcg gtgaggtcgt agatcagctg cgcgagatct tcgatcgagt
     4621 agatgtcgtg gtgcggtggc ggcgagatca gcgtgacgcc gggcgtcgag ttgcgcgcct
     4681 tcgcgatgcg atcgtcgatc ttgttgccgg ggagctgacc accttcgccg ggcttcgcgc
     4741 cctgcgcgac cttgatctgc agatcgtcgg cgttgacgag ataatgcgtg gtgacgccga
     4801 accggcccga cgcgatctgc ttgatcgcgg agcggcggaa gtcgccattc tcgtcgggga
     4861 cgaagcggtg cggctcctcg ccgccttcac cggagttgga cttcgcgccc atccggttca
     4921 tcgcgatcgc gagcgtctcg tgcgcctcgg cgctgatcga tccgaacgac atcgcgccgg
     4981 tcacgaagcg cgagacgatc gacttgatcg gctcgacctc gtcgagtgga atcggatcgc
     5041 caccttcgat gtcgagcaga ccgcgcagcg tgacgagcgc gttctcctcg tcgtcgcata
     5101 gccgctcgaa ctcctggaac ttcgcggcgt cgttcgtgcg caccgagtcc tggagcgcgg
     5161 cgatcgtcgc tggcgtccac ttgtgcagct cgccggtgcg ccgccagcga tactggccgc
     5221 cgatcggcaa ctgatcgtcg gttttcatat agccgcgagc gtggcgggcg accgcctcgt
     5281 cgccaagctc ctcgagcccg acgccgttga tgcgcgacgg ggtgccggtg aaccattggt
     5341 cgatcagatc gcgatcgaga ccgactgctt cgatgatctg cgagccgcga tagctatgca
     5401 ccgtcgagat gcccatcttg gacatcacct tgagcaagcc ctcttcgagt gccttgatgt
     5461 agagcttcgg ctccttgccc atcgcgcgca cggtgtcgat cgcgaggtac ggattgatgg
     5521 cagcagcgcc gtagccgatc agcagcgcca cgtcgtggac ttcgcggacc tcggcggtct
     5581 cgacgaccag accgacttgc atgcgaatac cctcgcgcac gaggtgttgt tgcaccgcgg
     5641 acaacgcgag cagtgacgga atcgcgacgc gctgcttgtc gcagccgcgg tcgctgagga
     5701 tcagcacgtt gtggccgtca tcgatcgctt gcacggcggc gcgacacagc ttctcgagtg
     5761 ccttctcgag ccagcccgcg ccgtggctca ccggccacag cagcgacagc gtgatcggct
     5821 cgaacacgcc ttcctgcgtc gcgcggatct tcgcgaggtc cttgctgtcg aggatcgggc
     5881 tgcgcagcgc cagctggtgg cactgctcgg gcgactcttc gaacgtgttg ccgtcgggac
     5941 cgagcgacga ctcgagcgac atcacgagcg actcgcggat cggatcgatc ggcgggttcg
     6001 tcacctgcgc gaacagctgg tggaagtatg tgaacaggct cggtgcacgt tgcgacaaca
     6061 ccgcgagcgg cgtgtcggta cccatcgagc cgaccggttc ctttccggtg tcggccatcg
     6121 gcgtgatgat gagatcgatg tcttcgtcgg tgtaaccgaa cgcgcgttgc tgctcgagca
     6181 acggtgcgcc ggtgatcgac ggcggtgcct cggcggcatc gagctcgtcg agctcgtaca
     6241 cgttcttgtc gagccacttg cgatacggga agcggccggc gacgtcacgc ttgagctcgg
     6301 cgtcgtcgac gatgcgacct tctgccgtat cgacgacgaa catcatgccg ggcgtcagcc
     6361 ggcccttctg ccgcacttgc tcgggcggta cgtcgatcac gcccgtctcc gacgcgagga
     6421 tcacgcggtc atccgtcgtg atcgtccagc gcgcggggcg taggccgttg cgatcgagcg
     6481 tggcgccgac gagctggccg tctgaaaaca cgatcgccgc cgggccatcc cacggttcga
     6541 gaagtgacga cgcgtagcga tagaagctct tgcggtcgtc gtccatcgcc gcgttccctt
     6601 cccaggcctc gggaatcatc atcatcatcg agtgcggcag cgtgcggccg ccgagcgtga
     6661 gcagctcgac catgttgtcg aactgcgccg agtcgctctt gccgggcacg atgatcggcc
     6721 acagccgctc gaggccgccg gagaacttcg cgctcttcag ctggctcttg cgcgcctgca
     6781 tccagttcgt gttgccacgc agcgtgttga tctcgccgtt gtgcgcgatg tagcggaacg
     6841 gctgcgcgag atcccaggtc ggaaacgtgt tcgtcgagaa ccgcgagtgc acgaccgcga
     6901 tcgcgctctc catgagcggc gacttcagat cggtgaagaa ctgcggcacc tgcgcgggca
     6961 gcagcagccc cttgtagacg atcgtctcgg tcgacaagct cgcgacgtgg aagtagcccg
     7021 acggatcggc gcgccgctcg cgcacgcggt tctcggtgag cttgcggatc acgtagagcg
     7081 tgcgttccca cgcgctgggc ggaacgcgcc gcatgcgcac gtagatctgg cggaacacgg
     7141 gcagcacgtc gcgtgcgatg cggccgacat ggcgcagatc gatcggcacg tcgcgccagc
     7201 cgatcacttg ctggccttcg tcgcggatcg cgctctcgag caagccttcg cacgcggcgc
     7261 gcagacctgg atcgggcggc aagaacacgt tgccgatcgc gaagcggcga tttgccggaa
     7321 tgtcgaagcc gagccgcgcg ccctcggcgc ggaaaaactt ctcgggtagc tgcatcaaga
     7381 tgccggcgcc atcaccggta tcggggtccg caccgcatgc agcgcgatgg gacaggcgtt
     7441 taaggacctc gaggccttgg tcgacgattg ctctcgatcg ttcgcccttg atgtgggcga
     7501 caaagccgac gccacaggcg tcgcgttccc cggcggtgaa gtccatgacg ccacggagat
     7561 taacgcagcg cgtcgcctac accaacccat ttctatatgc gcgacgcggg ttcaggtcac
     7621 aacgcaagtt ctgcaacgag atccccgggg ctcaactgct gaacctcagg gccgagctcg
     7681 cgcgcgtcga gggcgtagcc gaccgagcct ttcggaagcg gcatgccggt gacgagcgcg
     7741 aaccaggtcg gcgcgtcggt cgccggcaac gtgccggcgc gaccatcgtc ggccgcgcca
     7801 gacatcagac gcgcgaggcg catcgcttta ctctcgttgt cgcgccgccg gtacatcgtg
     7861 aacgaaccgt cgcgctcgcg aatatagatg acgttaccgc gtgccgcggc gtgaatgcac
     7921 gcgaggtccc atagcgtcgg cctggcgccg acgccgagcg catccgcgaa gtcgcggccg
     7981 acgtgtgcga gcgccgcgcg gtagccgccg acgtcatgca gctctctgcg gccgcggatg
     8041 cccacatcga gccagcgcag ccgccgctcg gcgagatcga tcgtgagcgg cacgttgatc
     8101 accgaacgtc cgcgcagatc gaagcgctgc atcaccgagc gcggatcgaa gtactggcct
     8161 tcgtcgggcg cgagcatcat gccggcgaag ccgtgggtga gctgctcgaa cggaacgtcg
     8221 ttgtagctga acaccaccat cacgagatgg
//
LOCUS       DASUFX010000343         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_53508,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000343 DASUFX010000000
VERSION     DASUFX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53508"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VFV99_13700"
     CDS             complement(<1..1532)
                     /locus_tag="VFV99_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060415.1"
                     /translation="MEDKVNILVVDDLPDKRLAFREVLDDLDENLVLVGSGAEALREL
                     LEREFAVIVLDVNMPEIDGIETAELVRKHGRTAHTPIIFVTAYADEMQTERGYELGAV
                     DYILTPVVPAILRSKVRVFVDLYRAQRRAAKVARAEAARAAAEAAQRRSDLIALASRE
                     LSSSLDLRASMERLLEVLVPSVGGCAVLSVDTGNGHLGLYRQDREPARLLEALTLLPP
                     GVRRAMVEVQSGGYPLEVRTDAGGSSHWPPWLRVAHVLPLRVGERNIGALAVGTVDHD
                     ALTLDERGLLEIATRAAIAFDNAQLYSTLGREMARAKSAEEKLQEANRRKDEFLAMLS
                     HELRNPLAPIVTSAQVLDKVAPNDARIAWVREVIERQVTHLAGLVDDLLDVSRITQGK
                     ITLHREAVELGKVIEHSLEIVRPFFDSKRHVLSVDVPETPVWVFGDFSRLSQIFSNVL
                     HNAAKYTADGGEIGLQVEVDRGSVSVRVRDNGIGIEPQFLPHVFEVFTQGYRGLDRSQ
                     GGL"
     gene            complement(1545..>3861)
                     /locus_tag="VFV99_13705"
     CDS             complement(1545..>3861)
                     /locus_tag="VFV99_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060416.1"
                     /translation="SHQAFLDQLMESIGIVIHTIEANTRTETLLTQSQSLAEELQQTN
                     QALEEKARQLAHQNQEVERKNTEVEQARQALEEKATQLALTSKYKSEFLANMSHELRT
                     PLNSLLLLSDKLAKNPEGNLSLRQVESAKTIHASGNDLLALINDILDLSKIESGTVVA
                     DISELRLLDLQRYVERTFRHVAEAKQVAFEVHLDPHVPRTMLTDIKRMQQIIKNLLSN
                     AFKFTHEGSVSLLVSPAAGGWSPDNEDLATAEQVVAFAVTDTGIGISPDKQQIIFEAF
                     QQADGSTSRKYGGTGLGLAISRELSKLLGGEIRLTSVPGEGSTFTLYLPVEAARPSRM
                     TRPSAQEFRSTPPAAPKAAAVQPLYIEEEAPGAAVNEAGDDRASIHPGDRTMLIVEND
                     LAFAKVLLDAARRRGFTGLVTAKAATAISLIKEYMPTIVSLDIFLPDMPGWRILDRLK
                     ADLSTRHIPICIVSTDDSRDRALSSGAIGFITKPVASTEVIDDAIEQLADYVARPRKR
                     VCLAMAPGGDLDVVQSRFAEAAIDVIATTEAAEICDLLRKDLIDCLVIESGSTDLQPA
                     DLIEVAEQRALTRQLSVVIYGTGGGDALSRWRRRDGMFALREARSLERLVDAVYFSLH
                     RSVSELSEGERQAIEGLHHDSRVLEGKKALVVDDDMRNIFALSTVLHDHGMEIISANN
                     GHEAIRLVEEDPSIDIVLMDIMMPEMDGLTTMREVRKLEQRKDLPIIAVTAKAMKGDR
                     EKCIEAGAWDYLSKPVDTIHLLGVLKSWLHR"
BASE COUNT          638 a         1274 c         1245 g          704 t
ORIGIN      
        1 agcccgccct ggctgcggtc gagaccgcga tagccctgcg tgaacacctc gaacacgtgc
       61 ggcaggaact gcggctcgat accgattccg ttatcgcgta cgcgaacgct gacgctgccg
      121 cggtcaacct cgacctggag accgatctcg ccgccatcag ccgtgtactt ggccgcgttg
      181 tgcagcacgt tgctgaagat ctggctcaag cgcgagaagt cgccgaacac ccagaccggc
      241 gtctcgggca cgtcgacgga caacacgtgg cgcttggaat cgaagaacgg ccgcactatc
      301 tcgaggctgt gctcgatgac cttgccgagc tcgacggcct cccggtggag cgtgatcttg
      361 ccctgagtga tgcgcgaaac atcgagcaaa tcgtcgacga gcccggcgag gtgcgtcact
      421 tgccgctcga tgacctcgcg tacccaggcg atgcgagcat cgttcggcgc caccttgtcg
      481 agcacctgcg ccgacgtgac gatcggcgcg agcggattgc gcagctcgtg cgacagcatc
      541 gcgaggaact cgtctttgcg gcggttcgct tcctggagct tttcttcggc cgacttcgcg
      601 cgcgccatct cccggccgag cgtgctgtaa agctgcgcgt tgtcgaacgc gatggccgcg
      661 cgggtcgcga tctcgagcag cccgcgctca tcgagcgtca aggcgtcgtg atcgacggtt
      721 ccgacggcga gcgcgccgat gttgcgctcg ccgacccgaa gcggcagcac gtgggcgacg
      781 cgcagccatg gcggccagtg gctcgagccg ccggcgtcag tgcgaacctc gagcggatag
      841 cccccgcttt ggacctcgac catcgcccgc cggactccgg gcggcagcag cgtgagcgcc
      901 tcgaggagac gagccggctc gcggtcttgc cgatacaacc ctagatgacc gttgccggtg
      961 tcgaccgaca acaccgcgca gccgccaacg ctcggcacga gcacctcgag cagacgctcc
     1021 atactcgcgc gcagatcgag tgacgacgac agctcccgac tcgcgagagc gatcaagtcg
     1081 gaccgccgct gcgcggcttc cgccgccgcg cgcgcggcct cggcgcgcgc aactttcgcg
     1141 gcgcgacgct gggcgcggta cagatcgacg aacacgcgaa ctttgctgcg caggatcgcc
     1201 ggcacgacgg gcgtcaggat gtagtcgact gcaccgagct cgtaccctcg ttcagtttgc
     1261 atctcgtcgg catacgccgt gacgaaaatg atcggcgtgt gcgccgtgcg cccatgcttg
     1321 cggactagct cggccgtctc gatgccgtcg atctccggca tgttgacgtc gagcacgatg
     1381 acggcgaact cgcgctcgag cagctcgcgc agcgcctcgg cgccggagcc gacgagcacg
     1441 aggttctcgt cgagatcgtc gagcacctcg cggaacgcca gccgcttatc gggcaggtcg
     1501 tcgacgacga gaatgttgac tttgtcttcc acgtgaaccg cgcgtcagcg atgcagccag
     1561 cttttcaata cgcccagcag atggatggtg tcgacgggct tcgacaggta gtcccacgca
     1621 cccgcttcga tgcatttctc gcggtcgccc ttcatggcct tcgcggtcac ggcgataatc
     1681 ggcagatcct tgcgctgctc gagcttgcgg acctcgcgca tcgtcgtgag gccgtccatc
     1741 tccggcatca tgatatccat cagcacgatg tcgatcgacg ggtcctcctc cacgaggcgg
     1801 atcgcttcat ggccgttgtt ggccgagatg atctccatgc cgtggtcgtg cagcaccgtg
     1861 gaaagagcga agatgttgcg catgtcgtcg tcgacgacca gtgccttctt gccttcgagg
     1921 acgcgactgt cgtggtgcaa accctcgatc gcttgacgct cgccttccga cagctcgctc
     1981 accgagcggt gcagcgagaa atagacggcg tccacgagcc gctcgagcga acgcgcctcg
     2041 cgcagcgcga acatgccgtc acggcggcgc cagcgcgata gtgcgtcgcc gccaccagtg
     2101 ccgtagatca cgaccgagag ctggcgcgtc aacgcgcgtt gctcggccac ctcgatgaga
     2161 tcggcgggct gcagatctgt cgagcccgac tcgatgacga ggcagtcgat caggtccttg
     2221 cgcagcaagt cgcagatttc ggccgcctcc gtcgtggcga tgacgtcgat ggccgcctcc
     2281 gcgaagcgcg actgcaccac atcgaggtcg ccgcccggcg ccatggccag acatacgcgc
     2341 ttgcgcggcc gcgcgacgta gtcggcgagc tgctcgatgg cgtcgtcgat gacctccgtg
     2401 gaagcgacgg gcttggtgat gaagccgatc gcgcccgacg acaacgctcg gtcgcgggaa
     2461 tcatccgtcg agacgatgca gatcgggatg tgccgcgtcg acagatcggc cttcaggcga
     2521 tcgaggatgc gccacccggg catgtcgggg aggaagatgt cgagcgacac gatcgtcggc
     2581 atgtactcct tgatcaggga gatagccgtc gcggctttgg ccgtgacgag ccccgtgaag
     2641 ccgcggcgcc gcgccgcgtc gagcagcacc ttcgcgaacg cgaggtcgtt ctcgacgatc
     2701 agcatcgtgc gatcgcccgg gtgaatgctc gcccgatcgt cgccggcttc gttgaccgcc
     2761 gcccccggcg cctcctcctc gatatagagc ggctgcaccg ccgcggcttt cggcgcggcc
     2821 gggggcgtcg agcgaaactc ctgcgcgctc ggccgagtca tgcggctcgg gcgcgccgcc
     2881 tcgactggca ggtacagcgt gaacgtgctg ccttcgccgg gcacgctcgt cagccgaatt
     2941 tccccgccga gcagcttcga gagctcgcgg ctgatcgcca gacccaagcc ggtgccgccg
     3001 tacttgcgcg acgtcgatcc atcggcctgt tggaacgcct cgaagatgat ttgctgcttg
     3061 tccggcgaga tgccgatgcc ggtatccgtc acggcgaacg cgacgacctg ttccgcggtg
     3121 gccagatctt cgttgtccgg cgaccagccg cccgcggctg ggctcaccag cagcgacacc
     3181 gagccttcgt gcgtgaactt gaacgcattc gacagcaagt tcttgatgat ctgctgcatg
     3241 cgcttgatgt ccgtcagcat cgtgcgcggc acatgcgggt cgaggtgcac ctcgaacgca
     3301 acctgcttcg cctcggccac gtgacggaat gtgcgctcga cgtaacgctg cagatcgagc
     3361 agccgcagct cgctgatgtc ggcgacgacc gtgcccgatt cgatcttcga gagatcgaga
     3421 atgtcgttga tcagcgcgag tagatcgttg cccgaagcat gaatcgtctt ggcggactcg
     3481 acttggcgca ggctcaagtt gccttccgga ttcttcgcta gcttgtcgga cagaagcagc
     3541 aagctgttga gcggcgtgcg tagctcgtgc gacatgttcg caaggaactc ggacttgtac
     3601 ttcgacgtca acgcgagctg ggtcgccttt tcttccagcg cttggcgagc ctgctcgacc
     3661 tcggtgttct tgcgctcgac ttcctggttt tggtgcgcga gctggcgcgc cttctcttcg
     3721 agcgcctggt tcgtctgctg caattcctcg gcgagcgatt gcgactgcgt gagcagcgtc
     3781 tccgtcctcg tgttcgcctc gatcgtgtga atgacgatgc cgatcgattc catgagctgg
     3841 tccaggaacg cctggtgcga a
//
LOCUS       DASUFX010000344         9494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_888248,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000344 DASUFX010000000
VERSION     DASUFX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888248"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFV99_13710"
     CDS             <1..650
                     /locus_tag="VFV99_13710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5060417.1"
                     /translation="TIDAVRAWLVIVVLLLGASCEKKPAAGTGAGSQAAPKDDEAARE
                     QARFDAERRPDKVVEALGIKPGSIVADVGAGSGLLTVHLARAVAPNGKVVATDVDGAV
                     LTLLRQRLEKANLATVVEPRVVAADTPGLEPATYDAILLAEVDHYFTDAPAWLKAAGT
                     ALKPGGRIVISNRIYHRQQSMANAKKAGLELVSESTPVPTHFIAVFTVPPEAKKK"
     gene            647..787
                     /locus_tag="VFV99_13715"
     CDS             647..787
                     /locus_tag="VFV99_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060418.1"
                     /translation="MNYAPLAMLVVLAGCPSDDGNPSVLWFAPNGSETELKLQDSEPS
                     PW"
     gene            902..1165
                     /locus_tag="VFV99_13720"
     CDS             902..1165
                     /locus_tag="VFV99_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060419.1"
                     /translation="MQFDLMHRRGADNTTITTWTHHFDPLATGFDAQKYELDMQGAKV
                     DFAVGDKLVFRYSASNTIMAEAWIPNGDGKLSNGRVPNIKLPQ"
     gene            1222..1383
                     /locus_tag="VFV99_13725"
     CDS             1222..1383
                     /locus_tag="VFV99_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060420.1"
                     /translation="MKLAASVLVVVLAGCAADGADSVGGAGTNPPTLWLAMGSGSVMK
                     LVDVEPHPY"
     gene            1441..1758
                     /locus_tag="VFV99_13730"
     CDS             1441..1758
                     /locus_tag="VFV99_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060421.1"
                     /translation="MPAGTYHFVLDCIVVSAVDVSFKLMVENAGNIVVLAEWSKHFDP
                     LSTGYDAQAYAVDKQAPAIKFTPGDQLVLRYSASSSSKADAWIPNGDGQLSHGRLPNL
                     TLP"
     gene            1833..2885
                     /locus_tag="VFV99_13735"
     CDS             1833..2885
                     /locus_tag="VFV99_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060422.1"
                     /translation="MRVGIACVVVCVAGCGRIAFDTRDDGGGGSGTTDVGASDVGIPP
                     GSYTRTISITAGARDVPAGYSISLALDHAMLVATGKSFTSGQDLGVLRGGLDIDRVLD
                     AGAQWNSPTTRIWFRTASVIPANTTNTEHVLVYGRDATTPVADPNQVFLVAEDFENGL
                     GGWQVGAGSWSIVPAAAHSGTGGVVMTGPLAGANRWIECVGLNAADIALESYWHVTGA
                     AGNDLGMGVRSAAQSSPRHYLINLEQANGLVLAKDTASGFMTLSPYVTPPTANTWTRV
                     AIMIAGQNARVLVNETQVIPAAGSTDVGSELATGSVSLYAYQLGNGQSWYVDDLTVRR
                     YVDPEPTTTLGPEQQN"
     gene            complement(2882..4135)
                     /locus_tag="VFV99_13740"
     CDS             complement(2882..4135)
                     /locus_tag="VFV99_13740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5060423.1"
                     /translation="MVSDGRMQRAATLAVVVAALGYFVDIYDLILFGTVRVPSLTALG
                     YKGDALREQGIFLLNCQMGGMLVGGIAWGMLGDKRGRLSVLFGSIIVYSLANLANGFV
                     NTIGQYAVCRLIAGIGLAGELGAGITLVSELMPKHSRGIGTTIVAAFGILGGVVAGIV
                     GGGMPMIYEGVHWRTAFYIGGGLGLGLLVLRIGVVESGMFHAVRQQAHVSRGNFFALF
                     TNKERFLRYLRVIAVGVPIWYAVGILVAFCNDLGAALGLPDKPRPATAFMIAYAGLAV
                     GDLAAGLLSQWMRSRRRALAIFIAGTALTVVAYFTFGGTSLVVFYAILAVLGLFTGYW
                     AVFATIGAEQFGTNLRATVATTSPNFVRGSVVLLTIMFEQLTKPLGLLGSAAAVGVFA
                     IGVAVVGLVGLRETFGVDLDYLEEH"
     gene            4165..6063
                     /locus_tag="VFV99_13745"
     CDS             4165..6063
                     /locus_tag="VFV99_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5060424.1"
                     /translation="MKHLALAVALVIASHASARAQGGEQRAWVKEVPNLETWKRYSKT
                     VGSDEFGKAVIDVKTNAIYFIDVNLFNIHADFVLGVLLKQPWTAANVREYNKNYERVK
                     PRFILCYLTHHLKVDKWTFSFWEGDKIGPDDVMRVSKRFQETFFMKSLPFRPDSPMQQ
                     AVANDVKKRGLKTITNDEIYKAATFQAFNKGRTVGKLRIVPVGTPFESLTFERTDIVL
                     LQESYPDITPVAGIIASQFSTPLSHVNLRAGAWKIPNAGDKKARDKYGKLDGKVVYYE
                     VTDTAITLREATAAEIKELENKIFKARHVDLPEADTKTTHLAMLTRMRAKDVLRYGTK
                     ASNLGEIVTANLEGVRVPPGFGVPFFYYVQHMQKNGLDKKVEELLTNPKFKDGEWRKT
                     ALEQLRTAITEAPIDPDTVDMLYKRVRLKLGGKGVFVRSSSNSEDLPGFNGAGLYDTI
                     GNVVGKKQLGEALKKVWASLWNLRAVDERTAFGIDHRQAYMAVLIQVGVNATAAGVLV
                     TKNLWDPGDDHSFTINSKWGLGERVVPGQKLPEQIIFDPTNDGTKIISRADDPVMLVF
                     DEHGGMKELPVPAGAGVILSEERAKKLSNMVEKFMPVFTHGQPLDVEWVLEGEDVWIV
                     QARPYVGS"
     gene            6063..6521
                     /locus_tag="VFV99_13750"
     CDS             6063..6521
                     /locus_tag="VFV99_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060425.1"
                     /translation="MRHAFALIVLAACEIQPAPKKQAVPPPPPPAAQVEAPKPVEPPP
                     PNAGSGSALPAPPKLEVTAACLEVGAKVASVFIDTEADPAQRSVKEQERGNMTRKIAE
                     ACTVQGWSDEARTCYLGTKTPAEIKACETKFPRPRPTQPPRPPSRVEGNP"
     gene            complement(6522..7388)
                     /locus_tag="VFV99_13755"
     CDS             complement(6522..7388)
                     /locus_tag="VFV99_13755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5060426.1"
                     /translation="MRKCNLACGYCNEYDKVSKPVPLADVLRWLDKLAELKTEIVTVS
                     GGEPMLHPDIEAIVAGIRERKMIAGLITNGYFLQPERIAGLNRAGLQYLQISIDNVRP
                     DDVSQKSLKVLDSKLVNLAAHAQFQVNVNSVLGSGCAHPEDALTIARRAKELGFSTSV
                     GIIHDGTGHLQALSQRERAIYDEIVAVGAGMYTRIRGFQDNLLDGKPNDWQCRAGARY
                     LYICENGLVHYCSQQRGYPGTPLAEYTRDDIAREFDTEKACAPFCTIGCVHRASVLDQ
                     WRGKQELAKKSA"
     gene            7555..8217
                     /locus_tag="VFV99_13760"
     CDS             7555..8217
                     /locus_tag="VFV99_13760"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5060427.1"
                     /translation="MRRFILLRLNNWWRAFVLALAAYAVLGMVVLALVPHLAGVFLLG
                     AYCIPANSVIPIPHEPAILYFAKFYDPFWCALAGTIGSLIACFADYAMVGAALRHRAL
                     AKTRNSALFQWSTKWMKRYPFAITVLFSFTPLPISIVRILAPAVEYNVRRYMLAQIVG
                     RFPRFYLLALVGHAVMIPNWILIGLGILLLATFFLGARSVEADDDDLDDDEPEPDYAN
                     AA"
     gene            complement(8218..8877)
                     /locus_tag="VFV99_13765"
     CDS             complement(8218..8877)
                     /locus_tag="VFV99_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5060428.1"
                     /translation="MTQRLRDELVDTAKQMSALGLTPGMSGNVSVRFNDAFYVTPSGM
                     PYEKIVADDVVKIEHDGTVRPGQRTPTTEWRLHMDILGARQDIQAIVHTHSLFCTTIS
                     TLRRPIPALHYMVVLAGSDEIPCAEYATFGSAQLALNAVSALHGGDACLLANHGMVAL
                     GTSLAKALRLAAEVETLAAQYWHAAQIGSPHILDGGELDKVRERFAEYGQAKSKTKRS
                     W"
     gene            complement(8894..>9494)
                     /locus_tag="VFV99_13770"
     CDS             complement(8894..>9494)
                     /locus_tag="VFV99_13770"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="s-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5060429.1"
                     /translation="TAGWLATVDWGTAIAPIYRAHEAGVPVEVWVDETRPRNQGLLTA
                     WELAQHGVAHTLIADNAGGHLMQHGDVDLCIVGTDRTTRSGDVCNKIGTYLKALAAHD
                     NHVPFFAAVPSPSIDWTIDEGRDIPIEERSGDEVRFIAGHDTGGRSARVQVVGQGSAV
                     ANPAFDVTPRRLMTGLITERGIAEPTRASLAALFPEHQR"
BASE COUNT         1702 a         3077 c         3148 g         1567 t
ORIGIN      
        1 ctacgatcga cgcggtgcgc gcatggctcg tcatcgtggt tctgctgctc ggcgcttctt
       61 gcgagaagaa gccggcggcg ggcaccggcg cgggcagcca ggccgctccg aaagatgatg
      121 aggccgcgcg cgagcaggcg cggttcgatg cggagcgccg ccccgacaag gtcgtcgaag
      181 cgctcggcat caagccgggc tcgatcgtgg ccgacgtcgg cgcgggctcg ggtctgctca
      241 ccgtgcatct cgcacgcgcg gtggcaccga acggcaaggt ggtcgcgacc gacgtcgacg
      301 gtgcggtgct caccttactc cggcagcggc tcgagaaagc gaacctggca accgtcgtcg
      361 agccacgcgt tgtggcggcc gacactcccg gcctggagcc ggcgacgtac gatgcaattc
      421 tgctcgctga agtcgatcac tatttcaccg acgcgccggc gtggttgaaa gccgccggca
      481 ctgcgctaaa gccaggtggg cgcatcgtga tctcgaatcg catctatcac cgccagcaaa
      541 gcatggcgaa cgcgaagaag gcgggcctcg agctcgtcag cgaatccacg ccggtgccca
      601 ctcacttcat tgccgtgttc accgttccac cggaggcgaa gaagaaatga actacgcgcc
      661 gctcgcaatg ctcgtcgtgc tcgccggatg tcccagcgac gatggcaatc catcggtcct
      721 gtggttcgcg cccaatggtt cggagacgga gctgaagctc caggactccg aaccgtcgcc
      781 ctggtgagcg ccaggcgtcg gggccggtgg ctctgaagaa gcagcattcg acctgaaggc
      841 gagcattcct tcggggacgt accacttcgt cctcgattgc atcgtcattg ccggcgtcga
      901 catgcagttc gatctcatgc accggcgcgg cgccgataac acgacgatca cgacgtggac
      961 gcatcacttc gatccgctgg cgacgggctt cgacgcgcag aaatacgagc tcgacatgca
     1021 gggcgcgaag gtagacttcg ccgtcggcga caagctcgtg ttccgctact cggcgagcaa
     1081 cacgatcatg gccgaggcat ggatcccgaa cggcgacggc aagctgtcca acggccgcgt
     1141 cccgaacatc aagctcccgc agtgagcgcg ggttagccga cgtcagcgac agcgcgccgc
     1201 ggatctcgca cttgcgtaga tatgaagctc gcggcgtccg tgctcgtggt ggttctcgct
     1261 ggttgcgccg ccgatggcgc ggatagcgtt ggtggcgctg gtaccaaccc gcccacgctg
     1321 tggctcgcga tggggtcggg cagcgtgatg aagctggtgg acgtcgagcc ccatccctat
     1381 tgagcacccg gcatcggggc cggtggctcc gaagaagcag cgttcgctct ccaaggcgat
     1441 gtgccggccg gcacgtatca cttcgtgctc gattgcatcg tcgtgtcggc tgtcgatgtc
     1501 tcgttcaagt tgatggtcga gaatgccggc aacatcgtgg tgcttgccga gtggtcgaag
     1561 cacttcgacc cattgtcgac gggctacgac gcgcaggcct atgcggtcga caagcaagcg
     1621 cctgcgatca agttcacgcc gggcgatcag ctcgtgctgc gttacagcgc gtcgagtagc
     1681 tcgaaggccg acgcgtggat cccgaacggc gacggccagc tgtcgcatgg ccggctcccg
     1741 aacctcacgc ttccttaggg ttagtcactg cgtgcccgct tgtttgggca actgtcggtg
     1801 cacgcagtgg ctgaccccgt tatgctgatc gggtgcgtgt cgggatcgcg tgcgtcgtcg
     1861 tgtgcgttgc gggttgcggc cgcattgcat tcgacacacg cgacgatggc ggaggtggca
     1921 gcggcacgac cgatgtcggt gcgagcgatg tcggcatccc gcccggctcc tatacgcgca
     1981 cgatcagcat cactgcgggc gcgcgagacg tgcccgccgg ctactcgatc agccttgcgc
     2041 tcgaccacgc gatgctcgtt gcgaccggca agtcgttcac gagcggccag gatctcggtg
     2101 tgctgcgcgg cggcctggat atcgatcgcg tgctcgatgc tggcgcgcag tggaattcgc
     2161 cgaccacacg aatctggttt cgcaccgcga gcgtgattcc cgcaaacacg acgaacaccg
     2221 agcacgtgct ggtctacgga cgcgatgcga cgacgcccgt cgccgacccg aaccaggtat
     2281 tcctggtggc cgaggacttc gaaaacgggc tcggtggctg gcaagtcggc gccggcagct
     2341 ggagcatcgt tcctgccgcg gcgcacagtg gtaccggcgg ggtcgtgatg accgggccgc
     2401 tcgccggtgc gaatcgctgg atcgaatgcg tcgggctgaa tgcagccgat atcgcgctcg
     2461 aatcatactg gcacgtgacc ggtgcagccg gcaacgacct cggcatgggc gtgcgcagcg
     2521 cggcacagtc gtcgccgcgg cactatttga tcaacctcga gcaggcaaac ggcctcgtgc
     2581 tcgcgaagga caccgcgagc ggcttcatga cgctgtcgcc gtacgtgacg ccaccgacgg
     2641 cgaacacgtg gacgcgcgta gcgatcatga tcgcgggcca gaacgctcgc gtgctcgtga
     2701 acgagacgca ggtgattccg gcggcgggct cgaccgacgt cggcagcgag ctggcgaccg
     2761 gctcggtgag cctctacgcg taccagctcg gcaacggaca atcctggtac gtcgacgatc
     2821 tgaccgtacg caggtatgtc gatccggagc cgacgacgac gctcggcccc gagcagcaga
     2881 actagtgctc ttcgagatag tcgagatcga cgccgaacgt ctcgcgtaac ccgacgagac
     2941 cgacgaccgc gacaccgatc gcgaagacac cgaccgccgc ggccgagccg agtagcccga
     3001 gcggtttcgt gagctgctcg aacatgatcg tgagcagcac gaccgagccg cgcacgaagt
     3061 tcggtgacgt cgtcgcgacg gtcgcgcgca ggttcgtgcc gaattgctcc gcgccgatcg
     3121 tcgcaaacac tgcccagtag ccggtgaaca ggccgagcac ggcgagaatc gcgtagaaga
     3181 ccacgagcga cgtgccgccg aacgtgaagt acgcgacgac cgtgagcgcg gtgccggcga
     3241 taaaaatcgc gagcgcccgc cgccgcgaac gcatccactg cgaaagcagg ccggctgcca
     3301 gatcgccgac cgcgagtccg gcgtacgcga tcatgaatgc ggtcgcgggc cgcggcttgt
     3361 cggggagtcc gagtgcggcg ccgaggtcgt tacagaacgc gacgaggatg ccgaccgcgt
     3421 accagatcgg cacgccaacc gcgatgaccc ggaggtagcg caggaagcgt tccttgttcg
     3481 tgaacagcgc gaagaagttg ccgcgcgaga cgtgggcctg ctggcgcacg gcgtggaaca
     3541 tgccggactc gacgacgccg atgcgaagga cgagcaggcc aaggccgagc ccgccgccga
     3601 tgtagaaggc ggtccgccag tgcacgccct cgtagatcat cggcatgcca ccgccgacga
     3661 tgccggcgac gacgccaccg agaatgccga acgcggcgac gatcgtcgtg ccgatgccgc
     3721 gggaatgctt cggcatcagc tcgctcacca gcgtgatgcc ggcgccgagc tcgcctgcga
     3781 ggccgatgcc cgcgatcagc cggcacaccg cgtactggcc gatggtgttg acgaagccgt
     3841 tcgcgaggtt cgcgagcgag tagacgatga tcgagccgaa cagcaccgag agccggccgc
     3901 gcttgtcgcc gagcatgccc cacgcgatac cgccaacgag catgccgccc atctggcagt
     3961 tgagcaggaa gatgccttgc tcacgcagcg cgtcgccctt gtagccgagc gcggtgaggc
     4021 tcggcacgcg gacggtgccg aacagaatga gatcgtagat atcgacgaag taaccgagag
     4081 ctgcgacgac gaccgcaaga gtcgcggcgc gttgcatacg tccgtcagag accacggcgc
     4141 gactatacgc gttataacgc tcatatgaag cacctggccc tggcggtagc tctcgtgatc
     4201 gcgagtcacg cctccgcgcg cgcacagggt ggcgagcagc gtgcgtgggt caaggaggtg
     4261 ccgaatctcg agacctggaa gcggtactcg aagacggtcg gcagcgatga gttcggcaag
     4321 gccgtcatcg atgtaaagac caacgcgatc tatttcatcg acgtaaatct gttcaacatc
     4381 cacgccgact tcgtgctcgg cgtgctgctc aagcagccgt ggaccgctgc gaacgtgcgc
     4441 gagtacaaca agaactacga gcgcgtgaag ccgcgcttca ttctctgcta cctcacgcac
     4501 cacctgaagg tcgacaagtg gacgttctcg ttctgggagg gcgacaagat cggtcccgac
     4561 gacgtgatgc gcgtgagcaa gcgcttccag gagacgttct tcatgaagag cctgccgttc
     4621 cgtcccgact cgccgatgca gcaggcggtc gcgaacgacg tcaagaagcg cggcctcaag
     4681 acgatcacga atgatgagat ctacaaggca gccacgttcc aggcgttcaa caagggccgc
     4741 accgtcggca agctgcgcat cgtgccggtc ggcacgccgt tcgaatcgtt gacgttcgag
     4801 cgcactgaca tcgtgctgct gcaagagagc tacccggaca tcacgccggt cgctggcatc
     4861 atcgcgtcgc aattctcgac gccgctctcg cacgtgaacc tgcgcgccgg cgcgtggaag
     4921 atcccgaacg caggcgacaa gaaggcacgt gacaagtacg gcaagctcga cggcaaggtc
     4981 gtctattacg aggtgaccga caccgcgatc acgctgcgcg aagcgaccgc tgccgagatc
     5041 aaagagctcg agaacaagat cttcaaggcg cgacacgtcg atctgccgga ggcagacacg
     5101 aagacgacgc acctcgcgat gctgacgcgc atgcgcgcga aggacgtgct gcggtacggc
     5161 acgaaggcgt cgaacctcgg cgagatcgtg accgccaacc tcgaaggcgt gcgcgtgccg
     5221 cccggcttcg gcgtgccgtt cttctattac gtccagcaca tgcagaagaa cggcctcgac
     5281 aagaaggtcg aggagctgct gacaaacccg aagttcaaag acggcgagtg gcgcaagacc
     5341 gcgctcgagc agctgcggac cgcgatcacc gaagcaccga tcgatcccga tacggtcgac
     5401 atgctctaca agcgcgtgcg gctcaagctc ggcggcaagg gcgtgttcgt gcgcagctcg
     5461 tcgaactccg aggacctgcc cggcttcaac ggcgccggcc tctacgacac gatcggcaac
     5521 gtcgtcggca agaagcagct cggcgaggcg ctcaagaaag tgtgggcgtc cctgtggaac
     5581 ctccgcgcgg tcgacgagcg gaccgcattc ggcatcgacc atcggcaggc gtacatggcc
     5641 gtgctgatcc aggtcggcgt caacgcgacc gcggccggcg tgctcgtcac gaagaacctg
     5701 tgggatccgg gagacgacca cagcttcacg atcaactcga agtggggcct cggcgagcgc
     5761 gtcgtgccgg gtcagaaact gcccgagcag atcatcttcg acccgacgaa tgacggcacg
     5821 aagatcatct cgcgcgcaga cgacccggtg atgctcgtgt tcgacgagca cggcggcatg
     5881 aaggagctgc cggttccggc cggcgcgggc gtgattctga gcgaagaacg tgcgaagaaa
     5941 ctgtccaaca tggtcgagaa gttcatgccg gtgttcacac acggtcagcc gctcgatgtc
     6001 gagtgggtgc tcgaaggcga ggatgtgtgg atcgtgcaag cccgcccata cgtggggtcg
     6061 tgatgcgtca cgcgttcgcc ttgatcgtgc tcgctgcgtg cgagatccag ccggcgccga
     6121 agaagcaagc agtgccgccg ccaccaccgc cggccgcaca ggtcgaagcg ccaaagcctg
     6181 tcgagccgcc cccgccgaac gcaggctcgg gctcggcgtt gcccgcgccg ccaaagctcg
     6241 aagtcaccgc ggcgtgcctc gaggtcggcg cgaaggtcgc ctcggtgttc atcgacaccg
     6301 aggccgatcc ggcgcagagg agcgtcaaag agcaggagcg tgggaacatg acgcgcaaga
     6361 tcgccgaggc gtgcacggtc caaggctgga gcgacgaggc gcgtacgtgc tacctcggca
     6421 cgaagacgcc cgccgagatc aaagcgtgcg agacgaagtt cccgcgccca cgaccgacgc
     6481 agccaccgcg gccgccatcg cgggtcgaag gcaatccgta gctaggccga cttcttggcg
     6541 agctcttgct tgccgcgcca ctggtcgagc accgacgcgc gatgcacgca gccgatcgtg
     6601 cagaacggcg cgcacgcctt ctcggtgtcg aactcgcgcg cgatgtcgtc acgcgtgtac
     6661 tcggcgagcg gcgtgcccgg gtagccgcgc tgctgcgagc agtagtgcac gagcccgttc
     6721 tcgcagatgt acaggtagcg cgcgccggcg cggcactgcc agtcgttcgg cttgccgtcg
     6781 agcaggttgt cctggaagcc gcgaatgcgc gtgtacatgc cggcgccgac cgccacgatc
     6841 tcgtcgtaga tcgcgcgctc gcgctgcgac agcgcctgga ggtggccggt gccgtcgtgg
     6901 atgatgccga ccgacgtcga gaagccgagc tccttggcac gcctcgcgat cgtcagcgcg
     6961 tcctcgggat gcgcgcagcc cgagccgagc accgagttca cgttgacctg aaactgcgcg
     7021 tgcgccgcga ggttgacgag cttgctgtcg agcaccttga ggctcttctg cgagacgtcg
     7081 tcgggcctca cgttatcgat gctgatctgt aagtactgca ggccggcgcg gttgagacca
     7141 gcgatccgct cgggctgcag gaagtagccg ttcgtgatga ggcctgcgat catcttccgc
     7201 tcgcggatgc ccgcgacgat ggcctcgatg tcgggatgaa gcatcggctc gccaccgctc
     7261 acggtcacga tctcggtctt caactcggcg agcttgtcga gccagcgcag tacgtcagcg
     7321 agcgggactg gcttggagac cttgtcgtac tcgttgcagt agccacacgc gaggttgcat
     7381 ttgcgcatgg gcacgatctg cgcgagcacc ggatgatggc gatcgagcaa tgctcgtacg
     7441 gcaatccctg gtccagccca aagcgcgtgt ccccgtttca gccacgtcca aatgcccatt
     7501 cttgagctac attaagcacg gtttttcggg gtacgcaggc actccgttcg gcaaatgcgt
     7561 cgcttcattc ttcttcgctt gaacaactgg tggcgtgcgt tcgtgctcgc gttagcggca
     7621 tacgcggtct tgggcatggt tgtgctcgcc ctggtgccac accttgccgg cgtgttcctg
     7681 ctcggtgcct actgcatccc ggcgaactcg gtgatcccga ttccgcacga gcccgcgatc
     7741 ctgtacttcg ccaagttcta tgacccgttc tggtgcgcgc ttgccggcac catcggcagc
     7801 ttgatcgcct gcttcgcgga ctacgcgatg gtcggcgcgg cactgcgtca tcgcgcgctc
     7861 gcgaagaccc gcaattcggc gctgttccag tggtcgacga aatggatgaa gcggtacccg
     7921 ttcgcgatca cggtgctgtt ctcgttcacc ccgctgccga tctcgatcgt gcgcatcctc
     7981 gcgccggcgg tcgaatacaa cgtgcgccgc tacatgctcg cgcagatcgt cgggcgcttc
     8041 ccgcgcttct acctgctcgc gctcgtcggc cacgctgtca tgatcccgaa ctggatcctg
     8101 atcggcctcg gtatcctctt gctcgcgacg ttcttcctcg gggcacggtc cgtcgaggct
     8161 gacgacgacg atctcgacga cgacgagccc gagcccgact acgcgaacgc cgcgtagcta
     8221 ccagctgcgc ttcgtcttcg acttcgcctg gccgtactcg gcgaaccgct cgcgcacctt
     8281 gtcgagctcg ccaccgtcga ggatgtgcgg cgagccgatc tgcgcggcgt gccagtactg
     8341 cgccgcgagc gtttcgactt cggccgcgag gcgcagcgcc ttcgcgagcg aggtgccaag
     8401 cgcgaccatg ccgtggttcg cgagtaggca cgcatcacca ccgtgcagcg cactcaccgc
     8461 gttgagcgcg agctgcgcgc tgccaaacgt cgcgtattcg gcgcatggaa tctcgtcgga
     8521 gccggcgagc acgaccatgt aatgcagcgc gggaatcggc cggcgcagcg tcgagatcgt
     8581 cgtgcagaac aacgagtgcg tatgcacgat cgcctggatg tcctgccgcg cgccgaggat
     8641 gtccatgtgc aggcgccact cggtcgtcgg cgtgcgctgg ccgggacgca ccgtgccgtc
     8701 gtgctcgatc ttgacgacat cgtcggcgac gatcttctcg tagggcatac ccgacggcgt
     8761 gacgtagaac gcgtcgttga accgcacgct gacgttcccc gacatgccgg gcgtcaggcc
     8821 gagcgcgctc atctgcttgg ccgtgtcgac cagctcgtcg cggaggcgct gcgtcatcgc
     8881 ccgtactttc gcatcagcgc tggtgctcgg ggaacagcgc agcgagcgat gcgcgcgtcg
     8941 gctcggcgat cccgcgctcg gtgatgaggc cggtcatcag gcggcggggc gtgacgtcaa
     9001 acgcagggtt tgcgaccgca ctgccctgcc cgaccacctg cacgcgcgcg gaacggcccc
     9061 cggtgtcgtg gccggcgatg aagcggacct cgtcgcccga gcgctcctcg atcggaatgt
     9121 cccgaccttc atcgatcgtc caatcgatgc tcggcgacgg cacggccgcg aagaacggca
     9181 cgtggttatc gtgcgccgcg agcgccttca ggtaggtacc gatcttgttg cagacgtcgc
     9241 cggagcgcgt cgtgcgatcg gtcccgacga tgcacaggtc gacgtcgccg tgctgcatca
     9301 ggtggccgcc ggcgttgtcg gcgatcaggg tgtgcgcgac gccgtgctgc gcgagctccc
     9361 acgccgtgag cagcccctgg ttgcgcggcc gcgtctcgtc gacccagacc tcgaccggga
     9421 cgccggcctc gtgcgcgcgg tagatcggcg cgatcgcggt gccccagtcg acggtcgcaa
     9481 gccaccccgc ggtg
//
LOCUS       DASUFX010000345        10836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_748712,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000345 DASUFX010000000
VERSION     DASUFX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748712"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..2061)
                     /locus_tag="VFV99_13775"
     CDS             complement(358..2061)
                     /locus_tag="VFV99_13775"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5060430.1"
                     /translation="MSAEIELSEQRGKAIEVATMLGDSVVDVKHCMDPHSGKITSRTW
                     ALLATGALCVLVSAIAFYVSVKTAAYNKGALEYWTHVAHKPAYAYRAQQLSGGYDWLA
                     FGGFAIGIVSLAGALLRIRDERKSPYYRIGTAPGVEQPLEGAPSPSFPLVAPHGDDFV
                     FNFGTGMTGEAIIDGRATPLAELAAKGHARPSMTQPGAFELPLALTSRIRTKVGQTQF
                     VVSPVAKPRRQSVPLFAGLQSRTMSYFAGSLGVHLGIVLLLSYIPVEAGTASIDLAMH
                     EETDIRAKSVIEENTPPEERDAVDDGGAGSEGASAQMALAEGAAGTTKSDRKDGHMRM
                     KNNNIDPAVARIQAIEEARTAGILGSVSLQSGDAFASLTATDNLSSGPDDANVYGAIY
                     GADGESFGYFGYGRSGFGAGGGCTGGTGCDGIIGTRPGYGKIGLGKFGRSGWDGPGGG
                     VLGTRKHPPGVPTPIIGQPDAKGGLDKSIIRRYIKRNIDKIKYCYEKQLLAKPGLEGT
                     VSVSFFITPAGSVTSSTGAGVDPTVANCVADVVSTIEFPRPDGGGVQVNYPFTFHPAG
                     Q"
     gene            2230..3795
                     /locus_tag="VFV99_13780"
     CDS             2230..3795
                     /locus_tag="VFV99_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5060431.1"
                     /translation="MLDVRGLRVALPDGRVLIDNIDLRIAPGEVVVLLGGSGAGKSTF
                     ARVLFEREELDDAGFDVVAATLDLDRAQLGLVPQRGALFDHLDVRGNLELALRQGDSK
                     DDPATWLERVGLERELAEAGTPVTKLSGGQAQRVAVARVLASRRKLLFLDEPSVGLDP
                     HRVRVLARLIREQVKALGVSAIVVTHDVALAAGVADRLFLLSLENRKLEQLFADRWPG
                     ALEEAGHSAEERGRWLVELEAALVDHLDKHGDKPPPQGTPKPRGVLTKLARVADPLLA
                     PFRVAAIAAVKAPVQLVKNPRDFAIIARRVIVQTLLRPLPFYAIVSTLIGYTVLFVVS
                     KVGGAGVRPDALLRQIGGSHVIALAPALSALLFVAASGSATNAWLGSMGLTKQTLALD
                     ALGVDRRSYLWAPAWLSLALSFFAVAALFALGMILGGMLVCESYGITNAWDLLTGDIL
                     DPRPERLRYAVRAAFLVWIYAWGIASDVVAKGGGPKPEADAVTRGMTSSVVACTLWVV
                     AWELVTVLAVFRL"
     gene            3792..5273
                     /locus_tag="VFV99_13785"
     CDS             3792..5273
                     /locus_tag="VFV99_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060432.1"
                     /translation="MKIATLAGIVIVAAHAAGFAWLAQKSGGSELMLTVPELAVRATG
                     ETPGLHRSYVRTEYRGGHVREIGTTELVGPFQDPKAPACSGRVVVGQKLLDAMAPVMK
                     DMVTEQMRGTDIFPVGSFQRVRSLSLEWARAESNPGDRRLLGSAGAPDGYVRANATVV
                     FDRGEVALLVAFIPQRSPTSLKFRIAAVADLEFNNSVLRWVSDKLGADRIATSVAREQ
                     IDDILVTTFAPPPPFELPGGQSLQFIYCDGPIEIVDGAYGALPFAVAFTPLPNAPQIL
                     PPRFGPGARPAPPPDTTLALDLDVDALNAMLFELWRTGWLDRELANVGLDRRFNTDPI
                     VTDYLSIRISPVRLALPPVIAPHAGALRLAADARVAIADGAHTTVGRAFGALDFHFVK
                     PATTDLPVSVDLGALELACERTPTTLVPCYSDLVGAIRDRGAELHGALTDAFAALLGD
                     IFIDRHLGTTGLPAELVIGAVVPSVTRGGTLHLQLAGTLVPVQ"
     gene            5291..6010
                     /locus_tag="VFV99_13790"
     CDS             5291..6010
                     /locus_tag="VFV99_13790"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5060433.1"
                     /translation="MDRDGFREALLAIMERKVHWAWPHFTSGKVAKERLHIHFEQEYA
                     TYVRDFPVMVGWAYVQCPIAEVRRDLAENLYEEETGGVIAGKPHPDLFLEYPRGLGMD
                     LSRFDHVELLPAARQYRAVLDEYTQRNGWSQAAAVSTIFIEGTDHERGELDPNAPKRP
                     APPLEQHPLVVHYGLPVDGLALTKAHRRVEGSHRAAAWRMILDHVPASEYSGVLAAME
                     RTLEAWRTYRDDVAAACGLTR"
     gene            6028..6963
                     /locus_tag="VFV99_13795"
     CDS             6028..6963
                     /locus_tag="VFV99_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060434.1"
                     /translation="MRCALLLVLAACAREAPEPVGPIGIKVPPGTPKRLCVKQPDEAA
                     AISHASGDKHIVQYCVGTAIDQCFSLELISGGLRRLSEQPAAQSPAIQAAAHVETTNP
                     ELKVCGTNGCKTLTPQVWPGAAPLHAATNGTVAAVMLGDAEAGKGYIEVYDVMKAKKL
                     ASFKYARGDFKCGEIGMLGDTIYVGTSMCANPSGRAALYTAKGKKIANVGGKPDFGMF
                     GNAYTQVEDTVWAFLEENGNRIALQDVVKGKVKKTIDVSALFESKMGNPGESAIVRLD
                     GGRLAVIAGTPANGSVAVVDVTSGEVTIVRAPLCT"
     gene            7070..8191
                     /locus_tag="VFV99_13800"
     CDS             7070..8191
                     /locus_tag="VFV99_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5060435.1"
                     /translation="MPGAATSVERSGRTAVVRVRGDVVVPTARQLYGVLRSLCRRRDV
                     KKVVVDFGHVGKLDSSGVAVVELIKRAMDHAGKQLALDHLDDHHTAAFELAPPRRAQP
                     AATDAGPTWLEGIGDRVLEIGDTLRGLGALVRDTLAQGWAVATRRRHIPEGSLGAHIL
                     TMGADAVFIVGLLSFLLGMTIAFQGAVQLQKFGAGVFVSDMVGWSMVREFAPLITAIV
                     LTGRTGAAIAAELGTMRVGSEIDALTTMGVSPIRFLVVPRMAALTFVQPALTLMAMFI
                     GIAGGFLVTALVLDLSPATFWAHITYRVALADFAQGLGKSLVFAWIIGITACHLGLRA
                     RGDASSVGRATTRTVVVSIFMIVVVDAVFATIITLARMR"
     gene            8188..8925
                     /locus_tag="VFV99_13805"
     CDS             8188..8925
                     /locus_tag="VFV99_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5060436.1"
                     /translation="MTPPLLQCVDVSVGYETPVLQHVDLEIGRGEIVALLGGSGCGKS
                     TLLRTVCGLVSPLAGDVRLFGESLYALAPDARNPLLRRIGVAFQQDALFSSMTIEENV
                     ALPLRELSKLPATIIREMVRMRLDLVGIGDLARRMPANVSGGQRKRAALARASILDPE
                     IIFADEPTAGLDPVVAAEIDSTLVRFRDALGSTIVVVSHELESIRATADRAIMLARGG
                     VAAIGTVDELQHSRDDAVFQFFHARAG"
     gene            8922..9752
                     /locus_tag="VFV99_13810"
     CDS             8922..9752
                     /locus_tag="VFV99_13810"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5060437.1"
                     /translation="MTTTKQQKVRIGLFAVAAGALLAVVLVAFAGVHFWRPRHRYYVE
                     FDSTVYGLEKGADVFLNGIRVGKVRDIALAPRDISHVRVALDIDKAAPIRSDTRAILQ
                     FAGITGLKIIDLRGGSFAAPPLPTGSTIPVGQTLLDRLEDQGMAMVDRSRELLDHANA
                     IIAKTDAIVNDLAEATDGAKLDDIMSQTRATAANLAQASASLRGLIDENRAGLRASIA
                     AIEQTAKRAGELVDGNQVKAAVSDLRQASRSFKELAREVRQRPSRLLFSKPEPDRKLP
                     "
     gene            9749..10384
                     /locus_tag="VFV99_13815"
     CDS             9749..10384
                     /locus_tag="VFV99_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5060438.1"
                     /translation="MTRAVLVLLVVAACSGKLPATRFYQLAEPAGKTWGSSGVALVVE
                     VLTTDSAYDDERIVYRVTPYRLDYYNYHRWSAAPGLLIANYLERAFEKSGHFGSVTRE
                     PNPAAPVTLGGRVIAIEEVDQTKTKWLGRIVLELTLTDSASGAVVWAEQFEETEPLPT
                     QSPEGLARALSVALERIANRAVPAVSLFATQTAKAHESMTATQSRAARLRP"
     gene            complement(10374..>10836)
                     /locus_tag="VFV99_13820"
     CDS             complement(10374..>10836)
                     /locus_tag="VFV99_13820"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5060439.1"
                     /translation="FDNGTLARKYITELWGKGNLALIDELVDDKIKLSDPIMQKTEGK
                     AAVKAHLEKMGQELTESSITIDEIAVAGDVVVVREKWSGVHKGDFFGVPAAGKRVTCD
                     AVDWLRFKDGKVVENYSYFDLYDVFRQLGAAPSPEQLKSAKPEAAAKTARA"
BASE COUNT         1743 a         3655 c         3579 g         1859 t
ORIGIN      
        1 ggcggcccca gcgggcaagc gatgacgtac gcctggggtt tcgagtggga atgatcagac
       61 caatcgcccg gatctgcctc gaagctcttc gcgagcgacg gtttctcgtt cagcttcgac
      121 acgtgccggg cggtgatcgt cgtcgacgca agcacgattg tcgccaacgt cacgccacga
      181 gtacgagcac gaaccgcatc acgcatatcc acaagacaac gcacgacgct gcgagttgcg
      241 tctacgtacc ggcttcagcg ccgcggttgt gctgcagaca aacgccgacg cggcgagcac
      301 cagtgcgatg ctcgccgcgc gatagaccgt gacttgcgtc gcgatcacga tgtctgctta
      361 ctggccagcc gggtggaacg tgaacggata gttcacctgc acgccaccgc cgtcgggcct
      421 cgggaactcg atggtcgaga cgacgtcagc gacgcagttc gcaaccgtcg ggtcgacacc
      481 agcgcccgtc gacgacgtca ccgagccagc cggcgtgatg aagaagctga ccgacacggt
      541 gccttcgagg cccggcttcg cgagcagctg cttctcgtag cagtacttga tcttgtcgat
      601 gttgcgcttg atgtagcggc ggatgatcga cttatcgaga ccgcccttcg cgtccggctg
      661 accgatgatt ggcgtcggca cgcccggcgg atgcttgcgc gtaccgagca cgccaccgcc
      721 cgggccatcc cagccactgc ggccgaactt accgaggccg atcttgccgt agcccggccg
      781 cgtaccgatg atgccgtcgc aacccgtgcc ccccgtacag ccaccgccgg cgccgaagcc
      841 actacggccg tagccgaagt agccgaagct ctcgccgtcg gcgccgtaga tcgcgccgta
      901 gacgttcgcg tcatcgggac cactcgatag attgtcggtc gcggtcagcg acgcgaacgc
      961 atcgccactc tgcagcgaga ccgagccgag gataccagcc gtgcgcgctt cttcgatcgc
     1021 ctggatgcga gcgaccgccg gatcgatgtt gttgttcttc atccgcatgt gaccgtcctt
     1081 gcgatcactc ttcgtcgtgc ctgcggcgcc ttcggcaagc gccatctgcg cgctcgcacc
     1141 ttccgagccc gcgccaccgt cgtcgaccgc gtcgcgctct tcgggcggcg tgttctcttc
     1201 gatgacgctc ttcgcgcgga tgtccgtctc ctcgtgcatc gcgagatcga tcgacgccgt
     1261 ccccgcctcg actggaatgt acgagagcag gagcacgatg ccgaggtgca cgccgagcga
     1321 gccagcgaag tagctcatcg tgcggctctg cagacccgcg aacagcggca cgctttggcg
     1381 acgcggcttc gcgaccggcg agacgacgaa ctgcgtctgg ccgaccttcg tgcgaatgcg
     1441 cgaggtcagc gcgagcggca gctcgaatgc accgggctgc gtcatcgacg gacgcgcgtg
     1501 gcccttggca gcgagctcgg caagcggcgt cgcgcgacca tcgatgatcg cctcgcccgt
     1561 catgccggtg ccgaagttga agacgaagtc atcgccgtga ggcgcgacga gcgggaagct
     1621 cggcgagggt gcgccttcga gcggctgctc gacgcccggc gcagtgccga tgcggtagta
     1681 ggggctcttg cgctcgtcgc ggatgcgcag caatgcgccg gcgagcgaca cgataccgat
     1741 ggcgaagcca ccgaacgcga gccagtcgta accgccgctc agttgctgcg cgcggtacgc
     1801 gtacgccggc ttgtgcgcga cgtgtgtcca gtactcgagc gcgcccttgt tgtaggccgc
     1861 cgtcttcact gacacgtaga acgcgatcgc cgacacgagc acgcacagcg caccggtagc
     1921 gagcagcgcc cacgtccgcg acgtgatctt gccgctatgt gggtccatgc agtgcttcac
     1981 gtcgacaacc gagtcgccga gcatggttgc gacttcgatt gctttcccgc gttgttcaga
     2041 tagctcgatt tctgcagaca tgtagacaga cctccgtcga ggcgcccgcg cgcctagccg
     2101 ctcacacgct tcactcagtt ggcgaatgcg ttgcggttct gcggattcgg acaacggagg
     2161 ttcgcctagg ttccggagat ttttggctgc aaccacctcc ggcgatcggt gtctgctacc
     2221 gttcgcgcga tgctcgacgt acgcgggtta cgcgtcgcgc ttcccgacgg acgggtgctg
     2281 atcgacaaca tcgatctgcg catcgcgccg ggcgaagtcg tggtgctgct cggcggatcg
     2341 ggcgcaggta aatcgacgtt cgcgcgcgtg ctgttcgagc gcgaagagct ggatgacgcc
     2401 ggattcgacg tggttgccgc gacgctcgat ctcgatcgcg cgcagctcgg cctcgtgcct
     2461 cagaggggcg cgctgttcga tcacctcgac gtgcgtggca acctcgagct cgcgctgcgg
     2521 caaggcgaca gcaaagatga tccggcgacg tggctcgagc gcgtcggact cgagcgcgag
     2581 ctcgccgagg ccggcacgcc agtgacgaag ctctcgggtg gtcaggcaca acgcgtggcc
     2641 gtggcgcgcg tgctcgcaag ccggcgcaag ctgctgttcc tggacgagcc gagcgtcggc
     2701 ttggacccgc atcgcgtacg agtgctcgcg cggttgatcc gcgagcaggt gaaggcgctc
     2761 ggcgtgtcgg cgatcgtcgt cacgcacgat gtcgcgctcg cggcgggcgt cgccgatcgg
     2821 ttgttcttgc tgtcgctcga gaaccgcaag ctcgagcagc tgttcgctga ccgctggccg
     2881 ggcgcactcg aagaggccgg gcattcggcc gaggagcgcg gacgctggct cgtcgagctc
     2941 gaggcggcac ttgtcgatca tctcgacaag cacggcgaca agccaccacc gcaaggcaca
     3001 ccgaagccgc gcggcgtgct gaccaagctc gcgcgcgtcg ccgatccgtt gctcgcgccg
     3061 tttcgcgtcg ccgcgatcgc agcggtgaag gcaccggtgc agctcgtgaa gaatccgcgc
     3121 gacttcgcga tcatcgcgag gcgtgtcatc gtgcagacgc tgctccggcc gttgccgttc
     3181 tacgcgatcg tatcgacgct gatcggctac acggtgctgt tcgtcgtcag caaggtcggc
     3241 ggcgccggcg tgcgccccga cgcgctgttg cgccagatcg gcggcagtca cgtgatcgcg
     3301 ctcgcgcccg cgttgtccgc gctgttgttc gtcgcggcaa gcggcagcgc gacgaacgcg
     3361 tggctcggct cgatggggct cacgaagcag acgctcgcac tcgatgcgct cggcgtcgat
     3421 cgccgcagct atttgtgggc gccggcgtgg ctgtcgctcg cgctgtcgtt cttcgcggtt
     3481 gcggcgctgt tcgcgctcgg catgattctc ggcggcatgc tcgtgtgcga gtcgtacggc
     3541 atcacgaacg cgtgggacct gctcaccggt gacatcctcg atccgcgacc cgaacggctg
     3601 cgttatgcgg tgcgcgccgc gttcctggtg tggatctacg catggggaat cgcgagcgac
     3661 gtcgtcgcga agggcggcgg accgaagccc gaagcggatg cagtcacgcg cggcatgacg
     3721 tcgagcgtcg tcgcgtgcac gctgtgggtc gtcgcctggg agctcgtcac cgtgctcgcg
     3781 gtgttccggc tgtgaagatc gcgacgctcg ccggcatcgt gatcgtggcc gcacacgcgg
     3841 cgggcttcgc gtggctcgcg cagaagagcg gcggcagcga gctgatgctc accgtgcccg
     3901 agctcgccgt gcgggcgacc ggcgagacgc ccggcctgca ccgcagctac gtgcgcaccg
     3961 agtatcgcgg cggccacgtg cgcgagatcg gcacgaccga gctcgtcggt ccgttccagg
     4021 atccgaaggc gcccgcatgc agcggccgcg tcgtcgtcgg ccagaagctg ctcgacgcga
     4081 tggctccggt gatgaaggac atggtcaccg agcagatgcg cggcaccgat atctttccgg
     4141 tcggctcgtt tcagcgcgtg cgctcgctat cgctcgagtg ggcccgcgcg gagtcgaatc
     4201 ccggcgatcg gcggttgctc ggcagcgctg gcgcgccgga tggctacgtg cgcgcgaacg
     4261 cgacggtcgt gttcgaccgc ggtgaggtcg cgttgctcgt cgcgttcatc ccgcagcgct
     4321 cgccaacgtc gctgaagttc cgcattgccg cagtcgccga cctcgagttc aacaactcgg
     4381 tcctgcgctg ggtcagcgac aagctcggcg ccgatcgcat cgcgaccagc gtcgcgcgcg
     4441 aacagatcga cgacatcctc gtcaccacat tcgcgccgcc gccgccgttc gagctgcccg
     4501 gcggccagtc gttgcagttc atctattgcg atggcccgat cgagatcgtc gacggtgcgt
     4561 acggcgcgct gccgtttgcg gtcgcgttca cgccgctccc caacgcgccg cagatcttgc
     4621 cgccgcgctt cggccccggc gcgcgccccg ccccacctcc cgacacgacg ctcgcactcg
     4681 atctcgacgt cgacgcactc aacgcgatgc tgttcgagct gtggcgcact ggctggctcg
     4741 atcgcgagct cgcaaacgtc gggctcgatc gccgcttcaa taccgatccg atcgttactg
     4801 actacctgtc gatccggatc tcgccggtac ggctcgcgct gccaccggtg atcgcaccgc
     4861 acgccggcgc gctccgcctt gccgctgatg cgcgcgtcgc gatcgccgat ggcgcgcaca
     4921 ccacggtcgg ccgcgcgttc ggcgcgctcg acttccactt cgtgaagccg gcgaccaccg
     4981 acctgccagt gtcagtcgac ctcggcgcgc tcgagctcgc atgcgaacgc acgccgacca
     5041 cactcgtccc ctgctacagc gacctcgtcg gcgcgatccg cgaccgcggc gccgagctgc
     5101 atggcgcact caccgacgca tttgctgcgc tcctcggcga catcttcatc gatcgccatc
     5161 tcggtacgac cggattgcct gccgagctcg tgatcggcgc cgtcgtgccg agcgtcaccc
     5221 gtggtggcac gttgcactta cagctcgccg gcacgcttgt cccggttcag tgacccggta
     5281 aacttgggct gtggatcgag acggtttccg ggaagccttg ctcgcgatca tggagcgcaa
     5341 ggtccattgg gcgtggccgc acttcacgtc cggcaaagtc gccaaagagc ggctgcacat
     5401 tcacttcgaa caggagtacg cgacctacgt acgtgacttt ccggtgatgg tcggctgggc
     5461 atacgtgcaa tgcccgatcg ccgaagtgcg ccgcgatctc gccgagaacc tgtacgaaga
     5521 agagaccggc ggcgtgatcg ccggcaagcc gcatccggat ctgttcctcg agtatccgcg
     5581 gggtctcggc atggacctgt cgcggttcga ccacgtcgag ctgttgccgg cggctcgcca
     5641 gtatcgcgcg gtgctcgacg agtacacgca acgcaacggt tggtcgcagg ccgccgcggt
     5701 ctcgacgatc ttcatcgagg gcaccgacca cgagcgcggc gagctcgatc cgaacgcgcc
     5761 gaaacgcccc gcgccgccgc tcgaacagca tccactcgtc gttcactacg ggcttccagt
     5821 cgacggcctc gcgctcacga aagcgcaccg ccgcgtcgag ggcagccacc gtgccgccgc
     5881 gtggcgcatg atcctcgacc acgtgccggc cagcgagtac agcggtgtgc tcgccgcgat
     5941 ggaacgcacg ctcgaagcgt ggcgaacgta tcgcgatgac gttgccgccg cgtgcgggct
     6001 cacgcgctga tcgattatca tcgccgcatg cgctgcgcgc tgctgctcgt cctcgccgcc
     6061 tgtgcgcgcg aggctcccga gcccgtcggg ccgatcggaa tcaaggtgcc acccgggacg
     6121 ccgaagcggt tgtgcgtcaa gcagcccgac gaggccgcgg cgatctcaca tgcaagcggc
     6181 gacaagcaca tcgtgcagta ctgcgtcggt accgcgatcg atcagtgctt ctcgctcgag
     6241 ctcataagtg gcgggctccg gaggctgtcc gagcagcccg cagcgcaatc gcctgcgatc
     6301 caagccgcgg cgcatgtcga gaccaccaac ccggagctga aggtgtgcgg caccaatggc
     6361 tgcaagacgc tgacgccgca agtgtggcct ggcgcagcgc cgcttcacgc tgcgacaaac
     6421 ggtacggtcg ccgcggtgat gctcggcgat gccgaagcgg gcaaaggtta catcgaggtc
     6481 tacgacgtga tgaaggcgaa gaagctcgcc agcttcaagt acgcgcgcgg cgacttcaaa
     6541 tgcggcgaga tcggaatgct cggcgatacg atctatgtcg gcacgagcat gtgcgcgaat
     6601 ccgtcgggac gcgccgcgct ctacacggcg aagggaaaga agatcgcgaa cgtcggcggc
     6661 aagcccgact tcggcatgtt cggcaacgca tacacgcagg tcgaggacac cgtgtgggca
     6721 ttcctcgagg agaacggcaa ccgcatcgcg ctgcaggatg tcgtgaaggg caaggtcaag
     6781 aagacgatcg acgtcagcgc gctcttcgaa tcgaagatgg ggaacccagg cgagtcagcg
     6841 atcgtcaggc tcgacggcgg ccgcctcgcg gtgatcgctg gaacacctgc gaacggcagc
     6901 gtcgcggtcg tcgacgtgac ctcgggcgag gtaacgatcg tgcgcgcgcc actttgtacg
     6961 taaccgcacg ccgtgcgtag gcgcacgcgc gctgcgctcg tgggcgcaca cggaacgtcg
     7021 cgtacccgtg cggttgcgct gtcctcgttc ttgcagtgga agccccgcaa tgcccggtgc
     7081 tgccacttcc gtcgagcgaa gcggacgcac ggcggtggtg cgcgtgcggg gcgatgttgt
     7141 cgtgccgacg gctcgtcagc tctacggcgt gctgcgcagc ctgtgcaggc ggcgcgacgt
     7201 gaagaaggtc gtcgtcgact tcggtcatgt cggcaagctc gattcgagcg gagtcgcggt
     7261 agtggagctg atcaagcgcg cgatggatca cgcgggcaag cagctcgccc tcgatcacct
     7321 cgacgatcac cacacggcgg cgttcgagct cgcaccaccg cggcgcgcgc agccggcggc
     7381 aaccgatgca ggcccgacct ggctcgaggg catcggcgat cgcgtgctcg agatcggcga
     7441 cacactgcgc ggactcggcg cgctcgtgcg cgacacgctc gctcaaggct gggccgtggc
     7501 aacgcggcgt cgccacattc ccgagggctc gctcggcgca cacatcctca cgatgggcgc
     7561 cgacgcggtg ttcatcgtcg ggctgctcag cttcttgctc ggcatgacga tcgcgtttca
     7621 aggcgccgtg cagctgcaga agttcggtgc cggcgtgttc gtttcagaca tggtcggctg
     7681 gtcgatggtc cgcgagttcg cgccgctgat cacggcgatc gtgctgaccg gacgcactgg
     7741 cgcagcgatc gcggccgagc tcggcacgat gcgggtcggc tccgagatcg atgcgctgac
     7801 gacaatgggc gtcagcccga tccggttcct cgtcgtgccg cgcatggccg cactcacgtt
     7861 cgtgcagccg gctctcacgc tgatggcgat gttcatcggc atcgccggtg gattcctcgt
     7921 caccgcgctc gtgctcgatc tgtctccggc gacgttctgg gcacacatca cgtatcgcgt
     7981 ggcactcgcc gacttcgcgc aagggctcgg caagtcgctc gtgttcgcgt ggatcatcgg
     8041 catcaccgcg tgccacctcg gcctgcgcgc acgtggagat gcgagcagtg tcggtcgcgc
     8101 gaccacgcgc accgtggtcg tcagcatctt catgatcgtt gtcgtcgacg cggtgttcgc
     8161 gacgatcatc acgctcgcga ggatgcgatg acgccaccac tgctccagtg cgtggacgtc
     8221 tcggtcggtt acgaaacgcc ggtgctccag cacgtcgatc tcgagatcgg tcgcggcgag
     8281 atcgtcgcgc tgctcggagg ctcaggctgc ggcaagtcga cattgctccg cacggtctgc
     8341 ggactcgtgt caccactcgc gggcgacgtg cggctgttcg gcgagtcgct gtacgcgctg
     8401 gcgccggacg cgcgcaatcc attgttgcgc cgcatcggcg tcgcgttcca gcaggacgcg
     8461 ctgttctcgt cgatgacgat cgaggagaac gtcgcactgc cgctgcgcga gctctcgaag
     8521 ctgcccgcga cgatcatccg cgagatggtg cggatgcggc tcgacctcgt cgggattggt
     8581 gacctcgccc gccgcatgcc ggcgaacgtc tccggcggtc aacgcaagcg cgccgcactc
     8641 gcgcgcgcat cgattctcga ccccgagatc atcttcgccg acgagccgac cgcgggcctc
     8701 gacccggtcg tcgccgccga gatcgacagc acgcttgtcc gcttccgcga cgcgctcggc
     8761 agcacgatcg tggtcgtcag ccacgagctc gagagcattc gtgcgaccgc cgaccgcgcg
     8821 atcatgctcg cgcggggtgg tgtggcagcg atcgggacgg tcgacgagct gcagcacagc
     8881 cgcgacgacg cggtctttca gttcttccac gcgagggcag gatgaccacg accaagcagc
     8941 agaaagtccg catcggattg ttcgcagtcg ccgccggcgc gctgctcgcg gtcgtgctcg
     9001 tcgcgtttgc tggcgtccac ttctggcgcc cacgccatcg ctactacgtc gagttcgact
     9061 ccacggtgta cggactcgag aaaggtgccg atgtgttctt gaacggcatc cgggtcggca
     9121 aggttcgcga catcgcgctc gcgccgcgcg atatcagcca cgtccgcgtc gcactcgaca
     9181 tcgacaaggc cgccccgatc cgcagcgaca cgcgtgcgat cctccagttc gccggcatca
     9241 cggggctcaa gatcatcgat ctgcgcggcg gctcgttcgc cgctcccccg ctccccaccg
     9301 ggtcgacgat tccggtcggc cagacgctgc tcgacaggct cgaggaccaa gggatggcga
     9361 tggtcgatcg gtcacgagaa ctcctcgatc acgcgaacgc catcatcgcg aagacggacg
     9421 cgatcgtgaa cgacctcgcc gaggccaccg atggcgcgaa gctcgacgac atcatgagcc
     9481 agacgcgcgc caccgccgcg aacctcgccc aggcgagcgc gtcgctgcgc ggcctcatcg
     9541 acgagaaccg cgccgggctg cgcgcgagca tcgccgcgat cgagcagacg gccaagcgcg
     9601 ccggtgagct cgtcgacggc aatcaggtca aagcagccgt ctccgacttg cggcaggcaa
     9661 gccgcagctt caaggaactc gcgcgcgagg tgcggcagcg accgtcacgc ctgctgttct
     9721 cgaaacccga gccggatcgg aagctgccat gacgcgtgcg gtgctcgtcc tgctggtggt
     9781 tgcggcatgc agcggcaagc tgcctgctac gcgcttctat cagctcgccg agccggccgg
     9841 gaaaacgtgg ggcagtagcg gcgtcgcgct tgtggtcgag gtgctgacga ccgacagcgc
     9901 atacgacgac gagcgcatcg tctatcgtgt gacgccctac cgcctcgatt attacaatta
     9961 ccatcgttgg agcgccgcgc caggcctcct catcgcgaac tatctcgaac gcgcgttcga
    10021 gaagagcgga cacttcggtt cggtcacgcg tgagccgaat cctgctgcgc cggtgacgct
    10081 cggcggtcgc gtgatcgcga tcgaagaagt cgatcagaca aagacgaaat ggctcggtcg
    10141 catcgtcctc gagctgacgc tgacggactc ggcctcgggc gcagtcgtgt gggccgagca
    10201 gttcgaggag acagaaccgt tgcctacgca gtcgcccgag ggcttggcgc gagcgctcag
    10261 cgtggcgcta gagcgcatcg cgaaccgcgc ggtgccagcg gtgtcgctgt ttgccacgca
    10321 gaccgcgaag gcccacgaat cgatgacggc gacgcagagt cgcgccgccc gccttaggcc
    10381 ctagcggtct tcgccgccgc ctcaggcttc gctgacttga gctgctccgg tgatggagcg
    10441 gcgccgagct gccggaacac gtcgtacaga tcgaagtacg agtagttctc cacaacttta
    10501 ccgtctttga accggagcca gtcgaccgca tcgcacgtga cgcgcttacc agccgcgggc
    10561 acgccgaaga agtcgccctt gtgaacgcca gaccacttct cgcgaaccac gaccacgtcg
    10621 cccgcaaccg cgatctcgtc gatcgtgatc gagctctcgg tcagctcctg gcccatcttc
    10681 tcgagatgag ctttcaccgc cgccttgcct tcggtcttct gcatgatcgg atcgctgagc
    10741 ttgatcttgt catccacgag ctcgtcgatc agcgccaggt tccctttgcc ccacagctcg
    10801 gtgatgtatt tccgagcgag ggtaccgttg tcgaat
//
LOCUS       DASUFX010000346        11845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1999931, whole genome shotgun sequence.
ACCESSION   DASUFX010000346 DASUFX010000000
VERSION     DASUFX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999931"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VFV99_13825"
     CDS             complement(<1..1575)
                     /locus_tag="VFV99_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060440.1"
                     /translation="MTQLQYLSTVLACGVFASACTAADKQPSGDDQGSDYGGDGSGSG
                     SGDGSGSGSGSGGVVDTDNNAGGPDVGADTMPTYPTAHPRIYLPKNQARLQAALAMNT
                     APAARFRLVVDRYINGEDIYGMKEWQVALMGALTNDAKYCTHAVNEVDSYVASEEAKI
                     AAGQQPTVAADSYLDVGWVIGDVMMTYDWCNARVSASQKTRWLRYANQAVWNVWHPAQ
                     AIWGSTPRAWSGWAVDDPANNYYYSFLRATAYLGLAAKGEDAQADTWITQFRDTKMLG
                     QLVPAFDAQLRGGGSREGTAYGVSMRDLFGLYDFWEGSTGEKLGAKTKHARQSMHSFM
                     HQMMPTLDRFAPTGDQARDSTATFFDYQRNYLQELIALYPQDALAPRAKAMLDNSSLP
                     VMARPELFVYDFLYDNAAVTAQPLAGMGTDYYASGIGHLYSRSGWDRQATWVELIGGP
                     YTQSHAHMDQGSLMIYKEGWLGYDGVISSFSGIIQDTGSHSLVSIKNASGPLRQITPT
                     MSQTVGVHAGSDWFYAA"
     gene            complement(1750..5412)
                     /locus_tag="VFV99_13830"
     CDS             complement(1750..5412)
                     /locus_tag="VFV99_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060441.1"
                     /translation="MKRVQVDWIVLITALFFAFGCGGGGCGGCGMEPIPGGFPSAKRN
                     PNAGQLRVTQSGLAAVSANPASLLGALGGAMNGVLKFNAPANCGGSTPICCPGGNPVN
                     PCGPIDIDLKMYPGDQPRLVLAPASGASRLDVTVRARVKTEMDIPVSVPLVGDCGVKI
                     DTSAGSVKDIRIDVPITFAQDATAGTTRVVVGTVNLTQLASEDVSLTGGIGCQLANLG
                     LSFFLGILTDQITGAVQSAIQDQTCKACESGNVAECGPFATACTDKVCMEGNECLQEL
                     GIAGRMRGSLLFASLSPGTTGAMDLYEVAGGYATTNNNGIALGLLGGMQPGGAARDKC
                     GPPATDPGLVTIPQSTFFQGNTRPDNGQAFDVGIGLHKSQLAQLAYAGYDGGLFCLTI
                     GASFAAQLSTDTISLLSRSLGKLVESNSPMAVGLRPQSPPTIILGKNTFKDDGMGNQV
                     IDVPLLDISFKALEIDFFAMVDEQYIRVFTVVADVHLPIGLQTTGMGQLQPVIGSTMD
                     AFTNITVKNNDAITESPAELAGLFPTLLELALPQLSSGIGPISLPELGGLKLNVTAIT
                     AVDNDSFLAIFANLAPGSMMAVAPVDTTVELAQVTEPADAVMRDIKQWESHKPPSVTL
                     ALSGSAPDLEYSYRVDDGSWSAWSASQRQTLSSRVFWLPGVHKVAVRSRQIGHPETLD
                     PEPAVVELPIGTGNSLPVVARSDFHGQAGASGCSCETSSGSSGALFALVIGLIVLPTR
                     RMRRRLRNLVIAGLRNGLRLGPIVWLTALALLPGCDCGNPCGSSDCLPGDVERGAIGR
                     WTSIAGDDKRVMVATYDQVLGDLVVVDATDQGALKYVAVDGVPDITPTHDPDTYRGGV
                     EDAGPNIGAWTSIALANGHAAVAYQDRDEKSLKYARETKPGSWKNHVIDGGDLEIGAY
                     ASLVFDADKRPAIAYIALGKDDGMGHRITELRLARSTSTEPDGADEWQSTVIASAPGS
                     CGGLCGSDACIAGAAATDPQVCVTPSTDCTATCANDEVCSAGACTAVITEPKLLEPPL
                     GTGLWVSLVVLPDGRLAAAYYDQSKRALTLAVEGGKGTSQFTETILDGNVTGADRGMW
                     SSAVVGNDGTIHIAYQDALGDQLMYTTWSGAPGTPEVVDDGQRPGDRTHPVGAAASIY
                     LNNATPSIAYQDGMTSDVYVATKGGAWTPSPLAQGPLLDGFSIGATTGHGTPVIAWGS
                     MDPAQSPPTLLTVRSP"
     gene            complement(5562..7211)
                     /locus_tag="VFV99_13835"
     CDS             complement(5562..7211)
                     /locus_tag="VFV99_13835"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5060442.1"
                     /translation="MRKTFFALALIAIAGCADKAYDPDAPAIDPNAPRVHITSPTRGQ
                     YAGDVTSITVTGNASDDSGEIASVEVNGVAATVAADGTWTAHVNVAAGTNLLHAIAKD
                     KQGNAGKESRAVVAGPQATLARSVPDAVTATLSAQTFDAIGRGAAGFIKSGNLMSAVQ
                     GMNPVVDVGDGPDCLYAQASITSMSVGDADILLAPQTGGIFLSGELTNVRVGMHLQWA
                     VSCLDGSRDIVIAAQKVSVQGNLVVGLVGRDFDIHLENQNVQITGFDLQLGGIPGTIV
                     DMLSLDTAMGPILGWATERFVVPLLNKSLAGLNDTKTIDVLGTQVNIDVKPSQINFTN
                     VGGQVLLNTSLRAQNDSGSFVYVANTLPTMDMSQGFQLAVADDAANQLLTSMWSAKGL
                     DKSIDFAGESYGEVGKLYDQVELETAVPPFVDATDGKLTLTIGDMIASFKSGGSIVTQ
                     VAINAQVELEVVKGMDGKLRFDVGTPTVYVDVMDESIEGSNQLSNAQFEAISSFALSR
                     IVAVGSGSVGAIPLPSIGGVSVTNLSIEEQHGYLIVDGEVQ"
     gene            complement(7181..7759)
                     /locus_tag="VFV99_13840"
     CDS             complement(7181..7759)
                     /locus_tag="VFV99_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060443.1"
                     /translation="MNTRALLTRCVAIALAVCLAGQPSWGHSFPPVRTVVVQIEGCEA
                     AVLVGYRPASGEATDTLLARIASEPKSQRLAAAKNLLAKEALAPLTFAVDKTPLVPTE
                     VRAKIGVDPGGTRPMVVVLVTFSVATSGSFAVTSREPRSTRISWTDRDSHRVDLEHAP
                     AQGRWFTGVASFLLSLGATGDSACAKHSSPSH"
     gene            complement(7756..10854)
                     /locus_tag="VFV99_13845"
     CDS             complement(7756..10854)
                     /locus_tag="VFV99_13845"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5060444.1"
                     /translation="MRLVVAAALVALLATCTGSAPPRSRAQRIGKLEEAIGGPHAIGR
                     VGDFILENDQVRFVIADTGRCPPNGDPNCVEVYGRVNTTYGGSLVDADLQRIAGDGKG
                     NDQLAELLPGFFFTAIDPTEVKVTNDGSDGRAAEVTVKGVGGDLFQMVSLLNTGLLYP
                     LTLEFTQTYKLEPGKHYVTIETTIKNVGMGAHPFPYLMPSELDVLTKFSIPGIGGLQL
                     SVPIGQLPLFGGEQKIFTPGVAGFNVRFAIEDSYKVAGGFPSFPGMVVDFVATKGEGV
                     SYGLAIPDSPDNYVNNYKSGYAAQEVTKYSMLLPFTYAGVTGAYQYRAPPMLGPNEEH
                     TYTSYFIVGRGDVGSIYDTILELRQASTGTFGGHVVDENTGQPVADADVIIRQGTKVI
                     DQLETDAHGAFLGKLLPGTYEYVVVRDDRPLTKSEQITITAGAQTGVHVVMGAPATLA
                     VSVVDELGRHAPAKIQLLGHDARIKTVDGRGILYSLQLGEGVRPTAFDGSDRYIEKAW
                     WTNDGRLLASVRPGTYDLVISRGPEYEITTESITLGPGSYSGKQLALTRSFDTPGWVA
                     GDFHIHAQPSTDSGLPIDERVTSCAAEGLEVAVATDHNFVTDYAPVIASSGLDPWLLG
                     IPGMELTTFETGHFIGYPLKVDPGSTRGGEFLWAHQGPQQLFDQLRKLAIDPTDNVVQ
                     VAHPRQQVLGYWAQFFVDFATGEPYTPTGIMGVFAPYGDEFKAENFSYDYDSVELLTG
                     RKIEDVHTYRAPNPLPPPPPNGFPDPQPVPGEIVVGKDGHPTFPGVVETWMTMLDRGN
                     KVTGIGASDSHHLLGDEPGDARTLLYVGDGKDTPGGFSRGDVVRATREHKAITTNAPF
                     VDISIKGQGIGSTVVESAASVPVEIHVRAPSWAKVDRLKLYMNSQIVQDMPITTGTDF
                     TTTVNVMPTADSWVVAEVTGTQSMFPVISAVEFPPLDATIILGALSAGLDLSALPITS
                     ALKPQRLHTATPYAITNPIWIDRDGNGWTPPKPPLPKKLVTPGSRPDVRAQFDALPEV
                     SP"
     gene            complement(10851..>11845)
                     /locus_tag="VFV99_13850"
     CDS             complement(10851..>11845)
                     /locus_tag="VFV99_13850"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5060445.1"
                     /translation="WEPYGYLRMQYIFVQNDPNVQFVGRDDGFELQNARIGVHGTMQG
                     IASFTVSLDGAVDERAQNTPQGKLRVGMRDAYADITLAGELQARGGYFQTWVDPVALV
                     PDTQREFVDKPLESRGMRSTEGWYTPGLPPGRSLGAAVRMAPTEPNHVGFELAVQNGA
                     DEFASNNDNDKPAVSAAAMLKLPNDGFLVAAGRWNPRTVGDLPFRQDETDLQGSVGGQ
                     VSIGPLVAGGGVMVQQTTFESTGGPKQNAFGGHAQAMVKVGATRPIAVGYRFGILDAS
                     SLITTDRVMEHTAGAVMAVPQFRMRVQLQFTHVQEQAARELSNDRLQLAAEVSL"
BASE COUNT         1943 a         3944 c         3908 g         2050 t
ORIGIN      
        1 cgctgcgtag aaccagtcgc tgccggcgtg aacaccgacg gtttgcgaca ttgtcggcgt
       61 gatctggcga agcggaccgc tcgcgttctt gatgctgacg agcgagtgcg agccggtgtc
      121 ctggatgatg ccgctgaacg agctgataac gccgtcgtaa ccgagccagc cttccttgta
      181 gatcatgagc gagccctgat ccatgtgcgc gtgggactgg gtgtacggtc cgccgatcag
      241 ctcgacccac gtcgcctggc gatcccagcc cgagcgcgag tagagatgac cgatgcccga
      301 cgcatagtag tcggtaccca tcccggcgag cggctgcgcg gtgacagccg cgttgtcgta
      361 gaggaagtcg tagacgaaca gctcggggcg cgccatcacg ggcagcgagc tgttgtccag
      421 catcgccttc gcgcgcggcg cgagcgcgtc ttgcggatac agggcgatca gctcctgcag
      481 gtagttgcgc tgatagtcga agaacgtcgc cgtcgagtca cgcgcctgat cgccggtcgg
      541 cgcgaaccga tcgagcgtcg gcatcatctg gtgcatgaac gagtgcatcg actggcgcgc
      601 gtgcttggtc ttcgcgccga gcttctcgcc ggtcgaaccc tcccagaaat cgtagaggcc
      661 gaacaggtcg cgcatcgaca caccgtatgc ggtgccttcg cgcgacccac cgccgcgcag
      721 ctgcgcgtcg aacgccggca cgagctggcc gagcatcttg gtgtcgcgga actgcgtgat
      781 ccacgtgtcc gcttgcgcgt cttcgccctt ggcggcgaga ccgaggtagg ccgtcgcgcg
      841 caagaacgag tagtaatagt tgttcgccgg atcatcgacg gcccagccgc tccatgcgcg
      901 cggtgtcgaa ccccagatcg cctgagcggg atgccacacg ttccacaccg cctggttcgc
      961 gtagcggagc cagcgcgtct tctgcgacgc cgacacccgg gcgttgcacc agtcgtacgt
     1021 catcatgacg tcgccgatca cccaaccgac gtcgagatag ctgtcggcgg cgaccgttgg
     1081 ctgctgccct gccgcgatct tcgcttcctc cgaggcgacg tagctgtcga cctcgttgac
     1141 ggcgtgcgta cagtacttcg cgtcgttggt cagcgcgccc atcagggcga cctgccactc
     1201 cttcattccg tagatgtctt cgccgttgat gtagcgatcg acgacgaggc gaaaccgcgc
     1261 ggccggtgcc gtgttcatcg cgagggctgc ttgcagcctc gcctggttct tgggcaggta
     1321 gatgcgcgga tgcgccgtcg gatacgtcgg catcgtgtcc gcgccgacgt caggtccacc
     1381 tgcgttgttg tccgtgtcga cgacgccgcc actaccgctg ccgctcccac tgccgtcccc
     1441 actaccgctg ccactcccat cgcccccgta gtcggagcct tggtcgtcgc cggaaggttg
     1501 tttgtctgcg gcggtgcaag cgcttgcgaa aactccgcac gctagcaccg tcgagaggta
     1561 ctgcaattga gtcatggccg cgcgacgtat caaacgcgca cgccgaaatc gactgtgtct
     1621 tttgtccgac actgcggtac aggcattcct cgcagcatcg attcctcgac gacaaccgtc
     1681 tacttcgatc gcgtcgcgcg gaccgcagat tacagatccg cgatcgatcg atcgtgaaat
     1741 aaattttgtt cagggcgagc gcaccgtcag caacgtcggc ggagactgtg cgggatccat
     1801 ggagccccaa gcaattaccg gagtgccatg gccggtcgtc gcgccgatgc tgaagccgtc
     1861 gagcagcggt ccctgcgcga gcggcgacgg cgtccacgca ccgcccttcg tcgcgacgta
     1921 gacgtcggac gtcatcccgt cctgatacgc gatcgagggc gtggcgttgt tgagatagat
     1981 cgacgctgct gcgccgaccg gatgcgtgcg gtcgccgggt cgctgaccat cgtcgacgac
     2041 ctccggcgtg ccgggcgcac cgctccacgt cgtgtacatc agctgatcac cgagcgcgtc
     2101 ctgatacgcg atgtgaatgg tgccgtcgtt gccgacgacc gcgctcgacc acatgccgcg
     2161 atcggcgccg gtcacgttgc catcgaggat cgtctcggtg aactgcgagg ttcccttgcc
     2221 gccctcgacc gcaagcgtga gcgcgcgctt cgattgatcg tagtacgcag ccgcaagccg
     2281 accgtcaggc aacacgacga ggctgaccca caggccggtg ccgagcggcg gctcaagcag
     2341 cttcggctcg gtgatgaccg ccgtgcacgc acccgccgag cacacctcgt cgttcgcaca
     2401 cgtcgccgtg cagtccgtgc tcggcgtgac gcagacctgc ggatccgtcg ccgccgctcc
     2461 ggcgatgcac gcgtcgctac cgcagaggcc accgcacgag ccaggcgcgc tcgcgatcac
     2521 ggtcgactgc cactcgtccg cgccatcggg ctcggtgctc gtgctgcgcg cgaggcgcag
     2581 ctcggtgatg cgatggccca tgccgtcgtc cttgccgagc gcgatgtacg cgatcgccgg
     2641 tcgcttatcg gcatcgaaca cgagcgacgc gtacgcgccg atctcgagat cgccgccgtc
     2701 gatgacgtga ttcttccagc tacccggctt ggtctcgcgc gcgtacttga gcgacttctc
     2761 gtcgcgatcc tggtacgcga cggcggcgtg gccgttcgcg agcgcgatcg acgtccatgc
     2821 gccgatgttc gggcccgcgt cttcgacgcc gccacgatac gtgtcgggat cgtgcgtcgg
     2881 cgtgatgtca ggaacgccgt cgaccgcgac gtacttgagc gcaccctgat ccgtcgcatc
     2941 gacaaccacg agatcgccga gcacctgatc gtacgtcgcg accatcacgc gcttgtcgtc
     3001 acccgcgata ctggtccagc gaccgatcgc accgcgctcg acgtcacccg gcaggcagtc
     3061 cgagcttccg cacgggttcc cacagtcgca gcccggcaac aacgcgagcg ccgtcagcca
     3121 cacgatcggc ccgagccgga gaccgttgcg gagacccgca atgacgaggt tgcggaggcg
     3181 gcgacgcatg cgacgtgtcg gcaacacgat caagccgatc acgagcgcga acagcgcgcc
     3241 gctgctaccg ctcgacgtct cgcagctgca gccactcgcg ccggcctggc cgtggaagtc
     3301 gctgcgcgcg acgaccggca acgaattgcc ggtgccgatg ggcagctcga ccaccgcggg
     3361 ctctggatcg agagtctcgg gatgaccgat ctggcgcgag cgcacggcga ctttgtggac
     3421 gccgggcagc cagaacacac ggctcgacag cgtctggcgc tggctcgcgc tccacgccga
     3481 ccacgagccg tcatcgaccc ggtacgagta ctcgaggtcg ggcgcactgc ccgacagcgc
     3541 gagcgtcacg ctcggcggct tgtgcgactc ccactgcttg atgtcgcgca tcacggcatc
     3601 ggcgggctcg gtcacctgcg cgagctcgac cgtcgtgtcg accggcgcga cggccatcat
     3661 cgagcccggc gcgaggttcg cgaagatcgc gaggaagcta tcgttgtcga ctgccgtgat
     3721 cgcggtgacg ttgagcttga ggccaccgag ctcgggcaag ctgatcgggc cgatgccact
     3781 cgatagctgc ggcagcgcga gctcgagtag cgtcgggaac aatcccgcga gctcggccgg
     3841 cgactcggtg atcgcgtcgt tgttcttgac cgtgatgttc gtgaacgcat ccatcgtgct
     3901 gccgatcacc ggctggagct ggcccatgcc cgtcgtctgc aggccgatcg gcaggtggac
     3961 gtcggcgacg acggtgaaca cccggatgta ctgctcgtcg accatcgcga agaagtcgat
     4021 ctcgagcgcc ttgaacgaga tgtcgagcag cggcacgtcg atcacctggt tgcccatgcc
     4081 atcgtccttg aacgtgttct tgccgaggat gatcgtcggc gggctctgtg gccgcaggcc
     4141 gaccgccatc ggcgagttgc tctcgacgag cttgccgagc gagcgcgata gcaggctgat
     4201 cgtgtcggtc gatagctgcg cggcgaagct cgcgccgatc gtcaggcaga acaggccacc
     4261 gtcgtaaccg gcgtaggcga gctgcgcgag ctgcgacttg tgcaggccga taccgacgtc
     4321 gaacgcctgg ccgttatcgg ggcgcgtgtt gccctgaaag aacgtcgatt gcggaatcgt
     4381 cacgagccct ggatcggtcg ccggcgggcc gcacttgtcg cgcgccgcgc cgcccggttg
     4441 catgccgccg agtagaccca gcgcgatgcc gttgttgttc gtcgtcgcgt agccgccggc
     4501 gacttcgtag aggtccatcg caccggtagt gccgggcgat agcgacgcga acaacaggct
     4561 gccgcgcatg cggccggcga tgccgagctc ctgcaaacac tcgttgcctt ccatgcagac
     4621 cttgtcggta cacgcggtcg cgaacgggcc gcactccgcg acgttgcccg actcgcaggc
     4681 cttgcacgtc tgatcctgga tcgcgctctg caccgcgccg gtgatctggt cggtgaggat
     4741 gccgaggaag aacgacaggc cgaggttcgc gagctggcaa ccgatgccac cggtcagcga
     4801 cacgtcttcg gacgcgagct gcgtcaggtt caccgtgccg accacgacgc gcgtcgtgcc
     4861 tgccgtcgcg tcctgcgcga acgtgatcgg cacgtcgata cggatatcct tcacgctgcc
     4921 agcactcgtg tcgatcttga cgccgcagtc gccgacgagc ggcaccgaaa ctggaatgtc
     4981 catctcggtt ttcacgcgtg cgcgcacggt gacgtcgagc cggctcgcac cggaggcagg
     5041 cgcgagcacg aggcgcggct ggtcgccggg atacatcttc agatcgatgt cgatcggacc
     5101 gcacgggttg accgggttac cgcccgggca acagatcggc gtactgccac cgcagttcgc
     5161 cggcgcgttg aacttgagca cgccgttcat ggcaccgccg agcgcgccga gcagcgaagc
     5221 agggttcgcg ctgacggccg cgaggccgct ctgcgtcacg cgcagctggc ctgcattggg
     5281 attgcgcttc gccgagggga agccacccgg aatcggttcc atgccgcatc cgccacagcc
     5341 accaccgcca cagccaaatg cgaaaaacaa cgcggtgatc aggacaatcc agtcgacttg
     5401 gacgcgcttc atcggtcccc ctcggcgaga ggtgtcactc gacagccccc atggggtcaa
     5461 gcgggaaccg ctgccagcgg tcgactgcgt cacaagttac gccaacaacg cctaacggag
     5521 acctatcgtc ccggggtcgg ccgaccgtct cggggacggc tctactgcac ttcgccgtcg
     5581 acgatgaggt agccgtgctg ctcctcgatc gacaggttgg tcaccgacac gccaccgatg
     5641 gagggcagcg gaatcgcacc gaccgagccc gagccgaccg cgacgatgcg cgacagcgcg
     5701 aacgaggaga tcgcctcgaa ctgggcgttc gacagctggt tcgacccttc gatgctctcg
     5761 tccatgacgt cgacgtagac ggtcggggtg ccgacgtcga agcggagctt gccgtccatg
     5821 cccttcacga cctcgagctc gacctgcgcg ttgatcgcga cctgggtgac gatcgagccg
     5881 ccgctcttga acgacgcgat catgtcgccg atcgtgagcg tgagcttgcc gtcggttgca
     5941 tcgacgaacg gcggcaccgc ggtctcgagc tcgacctgat cgtagagctt gccgacttca
     6001 ccgtaggact cgcccgcgaa gtcgatcgac ttgtcgagac ccttcgccga ccacatgctc
     6061 gtgagcagct ggttcgccgc gtcgtctgcg accgcgagct ggaagccctg cgacatgtcc
     6121 atcgtcggca gcgtgttcgc gacgtagacg aagctgccgc tgtcgttctg cgcgcgcagc
     6181 gacgtgttga gcagcacctg gccgccgacg ttcgtgaagt tgatctgcga cggcttgacg
     6241 tcgatgttca cctgcgtgcc gagcacgtcg atggtcttcg tgtcattgag gccggcgagc
     6301 gacttgttga gcagcggcac gacgaagcgc tccgtcgccc agccgagaat cgggcccatc
     6361 gcggtgtcga gcgacagcat gtcgacgatc gtgccgggga tgccgccgag ctggaggtcg
     6421 aagccggtga tctggacgtt ctggttctcg aggtgaatgt cgaagtcgcg gccgacgagg
     6481 ccgacaacga ggttgccttg caccgacacc ttctgcgcgg cgatcacgat gtcgcggctg
     6541 ccgtcgaggc acgacaccgc ccactgcagg tgcatgccga cgcgcacgtt cgtgagctcg
     6601 ccagagagga agatgccgcc ggtctgtggc gcgagcagga tgtccgcgtc gccgacgctc
     6661 atgctcgtga tcgacgcctg cgcgtagagg cagtccgggc cgtcgccgac atcgacgacg
     6721 ggattcatgc cctgcacggc cgacatcagg ttgccgctct tgatgaagcc agccgcaccg
     6781 cgaccgatcg catcgaacgt ctgcgccgag agcgtcgcgg tgaccgcgtc ggggacggaa
     6841 cgcgcgagcg tcgcttgcgg accggcaacc acggcgcgcg attccttgcc cgcgttgcct
     6901 tgcttgtcct tcgcgatcgc gtggagaagg ttcgtgccgg ccgcgacgtt gacatgcgcg
     6961 gtccacgtgc catcggcagc gaccgtcgcg gcgacgccgt tcacttcgac cgacgcgatt
     7021 tcgccgctgt cgtcgctcgc gttaccggtg acggtgatcg acgttacgtc gcccgcgtac
     7081 tggccgcgcg tgggagacgt gatgtgcacg cgcggtgcgt tcggatcgat cgccggcgcg
     7141 tccgggtcgt acgccttgtc agcgcaacct gcgatcgcga tcagtgcgag ggcgaagaat
     7201 gttttgcgca tgccgagtct cccgtggcgc cgagactcag caagaaggag gccacgccgg
     7261 tgaaccagcg tccctgggcg ggagcgtgct cgagatcgac ccgatgactg tcgcgatccg
     7321 tccacgatat ccgcgtacta cgcggctcgc gcgaggtgac cgcgaagctg ccgctggtgg
     7381 ctactgaaaa cgtcacgagc accactacca tcggccgcgt tcctccgggg tccacgccga
     7441 tcttggcgcg gacttctgtg ggcacaagcg gcgtcttgtc gaccgcgaac gtgagcggcg
     7501 cgagcgcttc cttcgcgagg agatttttcg cggcggcaag ccgttgtgat ttcggctcgc
     7561 tggcaatgcg cgccagaagt gtatcggtcg cttcaccgct tgcaggccgg tagccgacga
     7621 ggacggccgc ttcgcaacct tcaatttgca ctaccaccgt gcgcacgggc gggaacgaat
     7681 gaccccagga aggctggcct gccaggcata cggcaagtgc aatagccacg cagcgcgtca
     7741 gcagggcgcg cgtgttcatg gcgacacctc gggcagcgcg tcgaactgcg cgcgcacgtc
     7801 ggggcgagat cccggcgtga cgagcttctt cggcagcggc ggcttcggcg gcgtccagcc
     7861 attgccgtcg cgatcgatcc agatcgggtt cgtgatcgcg tagggcgtcg cggtgtgcag
     7921 gcgctgcggt ttgagggcgg acgtgatcgg cagcgccgag agatcgaggc cggccgacag
     7981 cgcgccgaga atgatcgtcg cgtcgagtgg cgggaactcg accgcagaga tcaccgggaa
     8041 catgctctgc gtgcccgtga cctcagcgac gacccagctg tcggcggtcg gcatcacgtt
     8101 gaccgtcgtc gtgaagtcgg tgccggtcgt gatcggcatg tcctgcacga tctggctgtt
     8161 catgtagagc ttgagccgat cgaccttcgc ccagctcggc gcacgcacgt ggatctcgac
     8221 cggcacgctg gcggcgctct cgaccacggt cgagccgatg ccctgcccct tgatcgaaat
     8281 gtcgacgaac ggcgcgttcg tcgtgatcgc tttgtgctcg cgcgtcgcgc gcacgacatc
     8341 gccgcgcgag aagccgcccg gtgtgtcctt gccgtcgccg acgtagagca gcgtgcgtgc
     8401 gtcacccggc tcgtcgccga gcaggtggtg cgaatccgat gcgccgatgc ccgtcacctt
     8461 gttgccgcga tcgagcatcg tcatccacgt ctcgacgacg cccggaaacg tcggatggcc
     8521 gtccttgccg accacgatct cgccgggcac gggctgcgga tcgggaaagc cgttcggagg
     8581 aggcggcggc agcggattcg gcgcgcgata cgtgtgcacg tcctcgatct tgcgcccggt
     8641 gagtagctcg acggagtcgt agtcgtagct gaagttctcg gccttgaact cgtcgccgta
     8701 cggcgcgaac acgcccatga tgcccgtcgg cgtgtacggt tcgccggtcg cgaagtcgac
     8761 gaagaactgc gcccagtagc cgagcacttg ctggcgcgga tgcgcgacct gcacgacgtt
     8821 gtcggtcgga tcgatcgcga gcttgcgcag ctgatcgaac agctgctgcg gaccttgatg
     8881 cgcccacaag aactcgccgc cgcgcgtcga gcccggatcg accttgagcg gatagccgat
     8941 gaagtgaccg gtctcgaacg tcgtcagctc catgcccggg atgccgagca gccacggatc
     9001 gagacccgag ctggcgatca ccggcgcgta gtcggtgacg aaattgtgat cggtcgcgac
     9061 cgcgacctcg agcccttcgg cagcgcagct cgtcacgcgc tcgtcgatcg gcaggccgct
     9121 atccgtcgac ggctgtgcgt gaatgtgaaa gtcacccgcg acccagcccg gcgtgtcgaa
     9181 gctgcgcgtg agcgcgagct gcttgccgct gtagctgccg ggcccgagcg tgatcgactc
     9241 ggtcgtgatc tcgtactcgg gaccgcgtga gatcacgagg tcgtacgtac cgggccgcac
     9301 actcgcgagc aaccggccgt cgttcgtcca ccacgccttc tcgatgtagc gatcgctgcc
     9361 atcgaacgcg gtcggccgca cgccctcgcc gagctgcagc gagtacagaa tgccgcggcc
     9421 gtcgacggtc ttgatgcgag cgtcgtggcc gagcagctgg atcttcgccg gcgcgtggcg
     9481 accgagctcg tcgacgaccg acaccgcgag cgtcgccggt gcacccatca cgacgtggac
     9541 gccggtctgc gcgccggcgg tgatcgtgat ctgctccgat ttcgtgagcg ggcgatcgtc
     9601 gcgaacgacg acatactcgt acgtgccagg cagaagcttg ccgaggaacg cgccgtgcgc
     9661 gtcggtctcg agctgatcga tcaccttcgt gccctgcctg atgatcacgt ccgcgtcagc
     9721 gactggctgc ccggtgttct cgtcgacgac atggccgccg aacgtgccgg tcgacgcttg
     9781 gcgcagctcg aggatcgtgt cgtagatcga accgacgtcg ccacggccga cgatgaagta
     9841 tgacgtatac gtgtgctctt cgttcgggcc gagcatcggc ggcgcgcggt actggtacgc
     9901 accggtcacg cccgcgtacg tgaacggcag cagcatcgaa tacttcgtga cctcctgcgc
     9961 cgcgtaccct gatttgtagt tgttgacgta gttgtcgggc gaatcgggaa tcgcgagccc
    10021 gtacgagacg ccctcgccct tggtcgcgac gaagtcgacg accatgcccg gaaacgacgg
    10081 gaagccaccc gcgaccttgt agctgtcctc gatcgcgaac cgcacgttga agccggcgac
    10141 gccgggcgtg aagatcttct gctcgccacc gaacagtggc agctgaccga tcggcaccga
    10201 tagctgcagt ccgccgatgc ccgggatcga gaacttcgtg agcacgtcga gctcgctcgg
    10261 catcaagtac ggaaacgggt gcgcgcccat gccgacgttc ttgatcgtcg tttcgatcgt
    10321 gacgtagtgc ttgccgggct cgagcttgta cgtctgcgtg aactccagcg tcagcgggta
    10381 cagaaggccc gtgttgagca gcgagaccat ctggaacaag tcgccgccga cgcccttcac
    10441 ggtgacttcg gcggcgcggc cgtcgctgcc gtcgttcgtg accttcacct cggtcggatc
    10501 gatcgcggtg aagaagaagc ccggcaacaa ctcggcgagc tgatcattgc ccttgccgtc
    10561 gccggcgatg cgctgcagat ccgcgtcgac gagcgagccg ccatacgtgg tgttgacgcg
    10621 gccgtagacc tcgacgcaat tcgggtcgcc gttcggcggg cagcgcccgg tgtcggcgat
    10681 gacgaatcgc acctgatcgt tctcgaggat gaaatctccg acgcgaccga tcgcgtgcgg
    10741 accgccgatc gcctcctcga gcttgccaat gcgttgtgcg cgcgagcgcg gcggtgccga
    10801 gcccgtgcag gtcgcgagca gcgcgacaag cgccgcggcg accaccagtc tcacagcgag
    10861 acctcagcgg cgagctgaag gcgatcgttc gacagctcgc gcgccgcctg ctcctggacg
    10921 tgagtgaact ggagctgcac gcgcatacga aactgtggca cggccatcac ggcccccgcc
    10981 gtgtgctcca tcacgcgatc ggtcgtgatc aggctcgacg cgtcgaggat gccgaaccga
    11041 tagccgaccg caatcggccg cgtcgcgccg actttcacca tcgcctgcgc gtgaccaccg
    11101 aacgcgttct gcttcggacc gcccgtcgat tcgaacgtgg tctgctgaac catcacgccg
    11161 ccgccagcca ccagcgggcc gatgctgacc tggccgccga cgctgccctg caaatcggtc
    11221 tcgtcctggc gaaacggcag atcgccaacg gtgcgcggat tccagcgtcc cgcggcgacg
    11281 agaaagccgt cgttcggcag cttcagcatc gccgccgccg agaccgccgg cttgtcgtta
    11341 tcgttgttcg acgcaaactc gtccgcgcca ttctgcaccg cgagctcgaa gccgacgtgg
    11401 ttgggctccg tgggtgccat gcgtacggcc gcaccgagcg agcggcccgg cggcaagccc
    11461 ggtgtgtacc agccttccgt cgagcgcatg ccgcggctct cgagcggctt gtcgacaaat
    11521 tcgcgctgcg tgtcgggcac gagcgcgacc ggatcgaccc aggtctggaa gtagccgccc
    11581 cgcgcttgca gctcgccggc gagcgtgatg tcggcgtacg cgtcgcgcat gccaacacgt
    11641 agcttgcctt gcggcgtgtt ctgcgcgcgc tcgtcgacag cgccgtcgag cgacaccgtg
    11701 aagctcgcga tgccctgcat ggtgccgtgc acgccgatgc gcgcgttctg cagctcgaag
    11761 ccgtcgtcgc gaccgacgaa ctgaacattc ggatcgttct gcacgaagat gtactgcatg
    11821 cgcaggtagc catacggctc ccacg
//
LOCUS       DASUFX010000347        28534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_748875,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000347 DASUFX010000000
VERSION     DASUFX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 28534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28534
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748875"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFV99_13855"
     CDS             complement(<1..544)
                     /locus_tag="VFV99_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060446.1"
                     /translation="MPNRLGVRTISLACLLALPLVACDKDKLKGPEAEVKKTDVKLDL
                     PAVPAFDLPPAPADGSHTVKELRVKGKKLLDTELTVHGFVTWAYDCKTAIRKPEETDK
                     DLQARIDEDPTLCERPKFYIGDTAQTPPEKSLWVVDVPRPYNALEVKRIKKKDRTDWD
                     RCEPTEKDPMKSVCPPYDVGD"
     gene            559..2265
                     /locus_tag="VFV99_13860"
     CDS             559..2265
                     /locus_tag="VFV99_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5060447.1"
                     /translation="MRLPVSQVGVVIALLALGVGACESPARVKPWRHAPDPTAEAAHA
                     PGSPVLAEDNSEAAVFAARPHTLRIHMDADPGRLTPVVDPSLWATRITVGTIFEPLLR
                     YVPPDAQGPARYTARLARSWRVMPSGLEIRIELQPGVTFHDGRPLTTSDVQFTLDAMR
                     TPHKGADHLRAMLDDVEAVELITPLEIRLRLKRPSSIVLRALAEIPILPMAIYDGSLL
                     AGGALVGTGPWKFGSNKNGIVHLVRNDKYWDGTPPIADVEFVVERDAAVALTAAKRGE
                     LDIIPALIPAHWPEQASAPGLAASFVPLQLAPPRLRYFQFNAARPLIADARVRHALAL
                     LIDRRGISKRVFDGLARPVNWPIWPGGPVDGAEAPVPDFDPAAAGKLLDAAGWVDTDK
                     DGIRDQGEGKQLKLVLYGVETPKVHDASGPPAKTERDYFVEAARRAGVVIEVKTGGES
                     WLDKKLGEGQWDIVEIAWNGRVDMDITPLVAGRTPIRPVQPRVDHALDAMAEAWDPAE
                     RGKLAKELADALAESWPIAGIVADAPQGLAHRRLQNVRVWNGWVDLSQLKFVDESKPS
                     SP"
     gene            complement(2335..3138)
                     /locus_tag="VFV99_13865"
     CDS             complement(2335..3138)
                     /locus_tag="VFV99_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060448.1"
                     /translation="MKTFLAIATTIGVFSAPAVAGNNKKNHRDTKPTLVAEKTHAPKA
                     DTKPDAKLDAKLDATKPIADAVAAPATASAAAMPGSLGAAAMPGSLGAALATGPALPL
                     PEVLDIPVLPADTSSVLAPYADLERRPLVTAKPLAKAAAKPKAPTLNMGKDYVLGGRQ
                     ATKVSERDTEVFVPKSLSQAQVATVVQAHMGDIHNCWELLPKAHRADACTAELRLSIS
                     DAGVVTDIELGGDVPAGAHKCITSAVSKWTFPTAETRTEIEYGISLRSL"
     gene            complement(3276..3602)
                     /locus_tag="VFV99_13870"
     CDS             complement(3276..3602)
                     /locus_tag="VFV99_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060449.1"
                     /translation="MLAASAIGCAVSYVPWLAAPTPLLSMLLMVPVGACVAPLYPLAA
                     AQAYARQPDASGAVLAAGHLFTPLALALPFLVGTVADHAGTLAALVLLLLQPLGLVVL
                     VGTTRR"
     gene            complement(3719..4021)
                     /locus_tag="VFV99_13875"
     CDS             complement(3719..4021)
                     /locus_tag="VFV99_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060450.1"
                     /translation="MGALRTSEQGESAAGEGDKDPAKDDRPVDSGAKIIIGPGTLDAP
                     ATPPRAVAPKAPVPQRASQSMPLTASRRGGLGLVILLYVLSAAALGFAIYERFIAT"
     gene            4048..5433
                     /locus_tag="VFV99_13880"
     CDS             4048..5433
                     /locus_tag="VFV99_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5060451.1"
                     /translation="MAHLLLIDDDAHGDAVVKALSKDGHEVRWVRTMADGLKQFRADR
                     ADVVLVDTDLPDGSGHDVLGQVRIEDPTTMVIMTSARGTIEEAVDAMKQGASDFVRKP
                     LDLADLLLRVSRSLSNRRLATQLEYLKARQARGSNIAGLVGECPEMRSVFGTVIRLAQ
                     KTGRRAGPVVLIVGETGTGKGVMARALHYNSSRRDGPFVEVNCASIPESLMEAEVFGA
                     ERGAYTGAVASHVGYVETAEGGTLFLDEIGCLSLPLQAKLLTVLESRTFRRVGSSVER
                     QADIQCVVATNMDLRAAVERGAFREDLLHRLEVIVLRMPPLRDRGPDRMRLAGHLRDE
                     ICREYGLSPRPFDDDVDDLVDDYHWPGNVRELRNCLEQILLLEDEPVIRARHFMLRGR
                     PKRSSNSRLGSEGGGIELEMPEEGLALQAVEDALIDRTLEKCDGNVSRAARMLGVSRD
                     QLRYRLAKRGS"
     gene            5586..7115
                     /locus_tag="VFV99_13885"
     CDS             5586..7115
                     /locus_tag="VFV99_13885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060452.1"
                     /translation="MRRKYCVACGSIYPQELRACPQDGSPLAFENTGHRLQLGHVLGN
                     YRLIRKLGEGGVGTVYEGEHVRLGRKMALKVLHPETASAETIVRFFNEARAVNEIRHP
                     NIVDVEDFVTTDDGEHYLIMELLKGEDLRGLISREGVMDPQRVSAIGEQIARALGAIH
                     RAGILHRDLKPDNIFLIRKPDGSEVAKLLDFGIAKFLTDKEGMTRAGMTLGTPEYMAP
                     EQIVSSGKPGPGADIYSLGMLMYECLAGAPAFTATTTAAILRGHISEPIVPPSHRRGE
                     PLPPVLESAVLKCLEKESAHRFADGDALAAALRSDRAVELSTPRYTPGKRQGRTLQML
                     PAFAMAAAAAVIHFLPRDDRAAAAPAPVVAMTAVPPSQPAPPALDPVPPPAPAVEEEP
                     AVPAPTHIEVTLVSNPEGAQLFLGKQALGESPAVATIPLGNQPVTLVARFSDGAEVTQ
                     TIVPDRPIAQIAFVKPQKQQSAAPPPPPSVKQKTTTQKPRASSGDKLESRDGTMDPFA
                     K"
     gene            7112..8602
                     /locus_tag="VFV99_13890"
     CDS             7112..8602
                     /locus_tag="VFV99_13890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060453.1"
                     /translation="MKSCPTCARLYIESEDTCALDTARLVEKTVRAPAGVGRLLGSYQ
                     LVCLLGEGGMGNIYVGRHLQLHRYVAIKVLREELAKRKENVARFFHEAHTINRLHHPN
                     IIESIDLVEDGGEGAYVVLELLKGHDLKTRLAHGALPIESAIHIGVQLADALGKVHAL
                     GVVHRDLKPENLILLDRDGRDDFVKLIDFGIAQIGFHGDGGIVGTAAYMAPEQGIANA
                     PVDQRADIYSLGVLLFEMVTGQHPFPSATDSEYMVQHADTAPPKPSKLAPRCPPALEA
                     VILRCLAKSPAERYGSADQVAAALRMVDPRKANGSRAAWVAFGLAFVGGAAAAAYFLL
                     PPYLATSTAEAKADVATVAQAEPAEPQHAVAAPPAPAQEAPAPAPPPPPAAPAIVEIG
                     IVSTPAGASVYRTGETVALGVTPFTVPLSRSDKPVQMRFEKAGFQPKTIEVPIAASLE
                     IEVSLAKQKQPASKATVQIRKPAAKPVTDTKPKPIQREGTIDPFAK"
     gene            8612..9787
                     /locus_tag="VFV99_13895"
     CDS             8612..9787
                     /locus_tag="VFV99_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060454.1"
                     /translation="MRALLASTMLMYVLGGGAAPAAAETDLLVLPVGKDAKELKPVLA
                     RVGTVSPKPIDASCASDPGCLNKAGADAGAHRVLSITASGTRYTLMLVDASTKLLLAT
                     RDIDVPKKRLAKDLGPVLSKFVDDAVVDKAKALFAEGNEHYNLGEFGPALERYKLAYR
                     VKPLPAFQFNIAQCHRKLGQYPDAIAMYQAYLVGVPSAQNKATVESLITETKKLLADQ
                     QALERQRESERLAAEQKKAEEARKATEAQAAADAETAKAEQARVAAERARDATYNRHP
                     ARGWMLLTTGLGLATGGAGAYFAVQARDEQRKFDALDCGDPIKKPLLDMATLDQCTDH
                     QKKGDRDAFLTNVLIGSGGAIVLASLIVFIADPGNVERPNRMNVSVTHNSIDLVVRW"
     gene            9781..14823
                     /locus_tag="VFV99_13900"
     CDS             9781..14823
                     /locus_tag="VFV99_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060455.1"
                     /translation="MVVITRALLALMLVGVAACRFDSKEFEDRSCTRDTDCRPDQSCV
                     AGLCTQKTCAASTDCGPDNTFQCTDGKCYADACSDDSACGPGYGCETGFCHVACVDHD
                     GDGIPFGTPCTGTQDCDDLDPTVTPGTVEGMYGDAICSDGKDNDCDGIKDGAELTCGA
                     QCANGDDDDDDGTLDFPDDPGCSSIDDNSEKGTAICDDGVDNDNDGKLDYRTDGTGDP
                     GCMAPTGASELSNTACNDGIDNDMDGTIDYRKDGTGDPGCVDDADTSERGTTACDDGI
                     DDDNDGTTDYRQGGGGDPGCTDPMDMNERGTTACDNDMDDDMDGRKDFNLNPAVGDAG
                     CSGPTDNDEHSTAACDDGMDNDNDGTTDWRAAGGDPGCADLADNSERGTGACDNGLDD
                     DGDGRTDYNLTASLTDPGCSDPNDANEKCSGIGCPACDNGVDDDADTRTDFSTTMAIS
                     DPGCSSPADTDEKCSGGTCPQCDDNMDNDTDTLFDLADPGCAGSPTGNNEASVSAACA
                     DGVDNDGDGRTDFGAGPTNDPGCSSATDTNEHDSSLVCDDGIDNDNDGRSDFNATPGA
                     GDPGCASVTDTSEKGATLCDDGMDNDNDGQIDFSVAGADPGCTGPADNNEHENTLRCD
                     DGTDNDGDGTADFQLSGGDAGCSSLQDSNERGNTACDNGVDDDADGRTDFNVMAALTD
                     PGCSSPTDSSEKGTTACDNSADDDADGLTDFSTNSAISDPGCSGPDDTNEKCTPGSGC
                     PQCDDGSDNDADTLVDLADPGCSGSPNGNNEASVSPACADGVDNDGDGRIDVGDDPGC
                     TSATDSSEKGTDVCDDGADNDTDGRTDYNVNSAIGDPGCSAPDDPTEKCSGGGCPQCD
                     DNTDNDNDGKSDYTLGGADPGCSGPTDISEHDAALVCDDGIDNDKDGVSDFQLSGGDP
                     GCSSLSDSSERGTATCDDGLDNDNDGRIDYNISAVLSDPGCTDPTDTSEKCTGVGCPA
                     CDNGADDDADTRTDYSTNPASSDPGCSGPDDTGEHCSGVGCPQCDDDIDNDADTLKDL
                     ADPGCAGNPTGNNEASVSPACADGIDNDGDGQTDSNDPGCASATDPSEKGTTLCDDGV
                     DNDLDGRTDFNVNAAVSDPGCSAPADPTEKCSGSGCPQCDDNTDNDNDGQADYTLGGA
                     DPGCSGPTDTTEKSTAPCDDGVDNDLDGLFDFQLSGGDPGCASLTDPSERGTVVCDDG
                     IDNDKDGLIDFATNGGDPGCSSVADTSERGVLACDDGMDNDLDGRTDYNLDGTQSDPG
                     CTGPADNNEKGPASLVCDNGIDDDTDGRTDYRTDSTGESCCTSPTGTTEQCACVADLD
                     GGTVHTCAAKTSGAAFCWGDNANGALGDGSTTDRQTPVQVLVSIGTPLTGVVHVATGD
                     LHSCALKSDGTVWCFGRNNAGQIGDMTNMARSFATPVMNLNGVIAIAAGWDHSCAVKS
                     DGTVWCWGANGNGQLGNGLSGNMNSSNVPVQVMGLTGATQVTAGELHTCARKSDGTAW
                     CWGRSNKGQLGNDTMMDSNVPVQVKYFQGTVKNLTGVTEVSAGQTHSCARLGTTEVRC
                     WGLNGNGQLGDNSMTDSARAVQVSNLANPASIAAGGSHTCAMLTTGTEVCWGFNMAGQ
                     LGNGMTMTSLVPVPVNTLTKIVTIGTGASHSCAALDTETTQCWGSDSMGQLGNGNGGS
                     SSSPGAVTLTCP"
     gene            complement(14824..16557)
                     /locus_tag="VFV99_13905"
     CDS             complement(14824..16557)
                     /locus_tag="VFV99_13905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060456.1"
                     /translation="MLKPLAKGGMAEVLLARAIGLAGFARHVVIKRIRVEHGSDEKSM
                     AMFLDEARLVAALHHGNIVQVHDVGEEDGKYFFVMEYVHGRDVRELLRHVKDKKEQIP
                     LEHVITIVSAAAAGLHYAHEQRGPDRQPLGIVHRDISPGNILIGFDGGVKVVDFGIAK
                     THVRAHDTQGGELKGKLGYMSPEQCRAQPLDRRTDVFLLGIVLYELCTVRRLFKADTR
                     YETMSLIVDGNVPPPSRFRKDLPDDLEKIIMRALANNPAERYQTADELRQTLDRFARV
                     AGLTASPGRLSDYMKEQFGDVLEPWLVDEAVPPPSAEVLERIRASAQLIPVSRASQPT
                     FDATPLPPPAPEPPELDVVADAKQAAAIERAAVAVVAALEPSAPLPAAAIEPTKPKRD
                     DSTKPELEAIKAAKPIAHGEPSGPVVPDVVYPVAPRSPFPWVWLVVFLLLAGGLAAGY
                     FFLYQDLGARSTPAAAPQRQDAAATPSTEPALDAASVEAVEAVDAAISEPVAEPPIDA
                     GAATVPVIDAGTAPAVKAPAKPPHPKPIRRKPKPPPAKPAVAKPAEPPPDAAPAEPPP
                     TPTAPPLDPAP"
     gene            16681..19398
                     /gene="glnD"
                     /locus_tag="VFV99_13910"
     CDS             16681..19398
                     /gene="glnD"
                     /locus_tag="VFV99_13910"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX5060457.1"
                     /translation="MATPAIAGTGVRAALDATFAALPAQLRVPGTGGAICRQIADVYD
                     HALAAHFAQATAGIGEPLALVATGGWARRELAPYSDIDFIVLHDRDEATAKQVSDRLL
                     YPLWDEKLAIGHAIREPRAAARLARGDLATATALLDARHIAGDRRLTNELVRATLSVL
                     SPGGNPNDLITLLATEKKARHDRFGASLYLLEPNLKQGIGALRDLSTALWAAQVRWHP
                     PRPGFDPESAESLITNLVKLGHLTRRQGDVLSFARDFLLRIRVLVQLTAKRRFDQLTF
                     EIQEAVAPSLYPDARPHEGDIRSAVAPAVEALMRDYYLHARGVVQVADLLLESARVPA
                     RRKPRIAEIDQSFITFNGELAIKDPRLFAERPSEMVRMFRVAVDQKLPVYGHTRELVA
                     ETIARDPVPLQRDPLAARMLLDALSDPRDSAQPSAFEVMHQLGILSALMPEWAPCTGR
                     VQHDLYHVYTVDQHQLYALAMQKRLARGELNEEHPYATELWREVKRPAALFLGTLLHD
                     VGKPLGKGHAEKGAVVAAAIAKRFGMTDDEIEVTEFLVRQHLTMAHLSQRRDLTDPDT
                     IARFAERVGSEDHLIKLYLLTLCDTAMTNPENLNAWKAGLLSELAIRTRRYFRGESLR
                     GESADTETEDGERAAREKILAIASGDGALDTELARGLVGGIDPRLISQLTARQAARHV
                     KLVAGAQHKQPPVGLEVHCFPMRGHSEVSIVAPDAPGVLAAIAGALTANRVDVLGAFL
                     GHIDLPDRRRLVMDTFYVRDLKGAAITDDDARWARLANDLRELLGGAPDPGAVAQLIQ
                     KRRPKSGMPKRHTPGVITEIKLHDDSSHSTIVEVFTQDRVGVLYAITQTLTDLGLDIA
                     LAKVSTEGEKVADAFYVHRAGERITDARERETLISRLRTALEAPGLTGG"
     gene            19395..19961
                     /locus_tag="VFV99_13915"
     CDS             19395..19961
                     /locus_tag="VFV99_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060458.1"
                     /translation="MSVVDLPPGIGLVGTPLGTFVAPRRARRLSAIALICVVAFGALT
                     WAFVSRHPYRWHASTQFVLPIAFAAIALLGFLVRRASVAVTRDGVRWGWASLNFTQEA
                     SRIVTAHVYHDGIALKAKRGSWWFLSARDWDRFDALVRQLRRAELPITDHEGRAPLRA
                     RLQSYGRFLDVLLVGSMLATFAVMLWAA"
     gene            complement(20212..21213)
                     /locus_tag="VFV99_13920"
     CDS             complement(20212..21213)
                     /locus_tag="VFV99_13920"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF006083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5060459.1"
                     /translation="MTRIGVFGAGAIGCWVGGKLSAAGLGGDVILVGRQRVMAELENG
                     LKVSDLTGGTREAKPTLATEASALATADLVLVTVKSAATAQAGRELAPHLAKSCVVVS
                     LQNGVRNVRVLRDALPDHRVLAGMVPFNVVRRAAGYYLRASAGTLMFDANDAIAPLSE
                     ACLKADLAFELRDDMAAVQWSKLVLNLNNAINALSGKPLAEELSDRDFRRCLAACQRE
                     ALALLKAAEQPLARITPLPTTWIAKMLTVPNGVFQLLGKRIVAIDPHARSSMCDDLDA
                     KRPTEIDYIQGEIVALADRLGLQAPVNARLVGLIKDAERGGKRDFTGAELYTALGRP"
     gene            21262..21384
                     /locus_tag="VFV99_13925"
     CDS             21262..21384
                     /locus_tag="VFV99_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060460.1"
                     /translation="MSDLIQSPKQESGAIGYILAFLIGIPIPILLAVFLIRGCD"
     gene            complement(21385..21645)
                     /locus_tag="VFV99_13930"
     CDS             complement(21385..21645)
                     /locus_tag="VFV99_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060461.1"
                     /translation="MIVAGDDPPPAPREEYVTARPGYVWIEGHWMRDHHRWHWVDGTY
                     ERERPGYVYAPGRWVRQGDRYVWVDGRWRTSNTVVIRGRGRF"
     gene            complement(21812..22195)
                     /locus_tag="VFV99_13935"
     CDS             complement(21812..22195)
                     /locus_tag="VFV99_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060462.1"
                     /translation="MDFDRSLLEMIDDYISDAMAKTGTRRAPRQTLQSLGREPELDVA
                     DLEPLADLIAARMRTKPNEPQGATRTFVIDERNADPALLAQVTQQLAQSQPALEPLPR
                     SRAPRSTSDLVEEEITNIVPRPAKR"
     gene            complement(22195..23307)
                     /locus_tag="VFV99_13940"
     CDS             complement(22195..23307)
                     /locus_tag="VFV99_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060463.1"
                     /translation="MRKLLSLFLLVAISGCPGSAPPNVADRPDAAGGGDDAAPTSFLL
                     SGKVMDYFGATAIEGAMVATDGLDPPLMTSSAADGAYSLDVAVGSSLFINASKANYRP
                     TRNAAMSVADMPVTKDVYLMTDQDVKNQYTAVGATPVLGTAILIAEMRRNDGTPLEGI
                     PLTNVTLLDAQNQPVATVKGPYFFNAAGSVDLALTTATAYQGRSRVAILDVPPGTYTL
                     AVTYLNGMNQNTTNNTPVTTVADGGTLAISGGMGGGGGGGGGNNVTDPSFATDIYPKL
                     QRAAAGGLGCANCHTLSGPAAVLKYDEAAGTVLANIIAATGVVNSASPADSLLLKRPL
                     YEAPPTPQDHPNATFLDVNDADYKLFLLWITKGAKP"
     gene            23471..24934
                     /gene="guaB"
                     /locus_tag="VFV99_13945"
     CDS             23471..24934
                     /gene="guaB"
                     /locus_tag="VFV99_13945"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5060464.1"
                     /translation="MLEEPLREALTFDDVLLVPSFSEVIPRDVDVATRLTPAIPLRIP
                     LVSSAMDTVTEAATAIRMAREGGIGFIHKNLTIEQQAREVHRVKKAQSGIVVDPLTIA
                     PSRTLEDALAIMRANRISGLPVVDGDRRPVGILTNRDVRFEKRLDVQVGEVMTKNPVT
                     VKEGVQIDEAKDLLHKHRIEKLLVIDGSGKLKGLITIRDIEQAQEHPTAVVDDLGRLR
                     VGAAVGVGPDREARIEALVAAGCDLICVDTAHGHSAGVIAAVRDIRKKFPKLQLVAGN
                     VATAEATRALIETGVNAVKVGVGPGSICTTRIVAGVGVPQLTAIADAVGAAKGSGVAI
                     IADGGIKYSGDVAKALAAGADTVMIGSLFAGTDEAPGEVILYQGRSYKSYRGMGSIGA
                     MQEGSKDRYFQADVEAPQKLVPEGIEGRVPYKGALRESLYQLIGGLRSSMGYLGCATI
                     AELHAKARFVKISASGLRESHVHDVIITKEAPNYRVE"
     gene            24977..26533
                     /gene="guaA"
                     /locus_tag="VFV99_13950"
     CDS             24977..26533
                     /gene="guaA"
                     /locus_tag="VFV99_13950"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5060465.1"
                     /translation="MAHQLVLVLDFGSQYTQLIARRIREQSVYCEIHPYTLANDLAKV
                     RALAPIGIVLSGGPNSVYDEGAPSVPAGIFELGIPVLGVCYGAQLTALVLGGKVRSAE
                     RREYGRATVRVLKGDEGVFRTVHAGEELAVWASHGDHVDAIPPGFVHTAESDNCKFSG
                     FVHAARKIHCVQFHPEVVHTPRGAELYSNFLFGVCGAKGDWSMASFVDEAVERIRAQV
                     GDNGRVICGLSGGVDSSVAAALIHRAIGNRLTCIFVDNGVLRKGEREQVAVIFRDHFH
                     VDLRVIDAEERFLKALADVTDPEKKRKIIGYEFIAVFEEQSKSIENAKFLAQGTLYPD
                     VIESVSFKGPSHTIKSHHNVGGLPERMQLALVEPLRELFKDEVRQLGLELGLPKHMVW
                     RQPFPGPGLAVRCLGNLTRARLDVLRNADAIIEEEIRAAGLYESIWQSFGVLLPVRSV
                     GVMGDARTYEEALALRAVHSRDGMTADWVQLPYDVLGRISSRIINEVRGINRVVYDIT
                     SKPPGTIEWE"
     gene            26541..26963
                     /locus_tag="VFV99_13955"
     CDS             26541..26963
                     /locus_tag="VFV99_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060466.1"
                     /translation="MNELALQPGERILKSDGEVIWLKGWFAKPRGRLVFTTQRLVFEL
                     PKAVAVTAGQALMNEMVNHLPIDVPRDRLDAVELGKYRKSDNVIVVRTEDAEYKFLFR
                     TPVKEWEATLKQALHDDKVALQHLLDRLKPKENPPPYR"
     gene            27016..27648
                     /locus_tag="VFV99_13960"
     CDS             27016..27648
                     /locus_tag="VFV99_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060467.1"
                     /translation="MLLRTSIAMLMLVCATAHAEVKVDWARGLVTADAVGVADRHAPN
                     PAVARGTSRRGAEDAAKKLLAAKVGELPVAGGSTVAAKAKDKAVKERIERAVANAITL
                     AADPETDGAWKVTLAVPIEAVRQAIAGPRALPADGDKGPVVVVVDSATAKPAVGWTIA
                     GVEAPVLWVTDVPAWAKDAPHATAKGAKGGAIEVTGIDASAATLFVVVTK"
     gene            27724..>28534
                     /locus_tag="VFV99_13965"
     CDS             27724..>28534
                     /locus_tag="VFV99_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060468.1"
                     /translation="MIRCVLPLAMVAALVSVAHAEDVVAYQAEGDAPTASADARVMAL
                     DEAFAHAVGNALADLVAGDVRTARKGELDREIVGRARLWVAKFAVTKDETVDNRRELT
                     VSVKIDRDKMRARLAELKIATKDAGTATEPAPAQRTATILMRLALPAGVRAVYGPNAV
                     QDAPGLIALTNLLRNAGFAVRRAPASGPAPRADGDLPLSDEEADALANEAKADVMAIA
                     GVSLGPPVVVRGQPTNAALVTAHVRIVDRKDKKVLGQGAAAAAARGEDPGYA"
BASE COUNT         4914 a         9451 c         9685 g         4484 t
ORIGIN      
        1 cgtccccgac gtcgtacggc gggcacacgc tcttcatcgg atccttctcg gtcggctcgc
       61 agcgatccca gtcggtgcgg tccttcttct tgatgcgctt gacctcgagc gcgttgtacg
      121 ggcgcggcac gtcgacgacc cagaggctct tctcgggcgg cgtctgcgcc gtatcgccga
      181 tgtaaaactt cgggcgctcg cacagcgtcg gatcctcgtc gatgcgggcc tggagatcct
      241 tgtcggtctc ttcgggcttg cgaatcgccg tcttgcagtc gtacgcccac gtcacgaagc
      301 cgtgcaccgt gagctcggta tcgaggagct tcttgccttt gacgcgaagc tctttgaccg
      361 tgtgcgatcc gtcggcggga gccggcggta ggtcaaatgc cgggacggcc ggcaggtcca
      421 gcttcacgtc ggtcttcttg acctccgctt cgggcccctt gagcttgtcc ttgtcacacg
      481 ctacgagtgg cagtgccaga aggcatgcca atgagatggt gcggaccccc agacggttcg
      541 gcatgctgcg gtaataacat gcgcctccca gtgtctcaag tcggcgtggt cattgcgctc
      601 ctcgcactgg gggtgggggc ctgcgagtca cccgcccgcg tcaagccatg gcggcatgcg
      661 ccggatccga ccgccgaggc tgcgcacgcg cccggctcgc cggttctcgc cgaggacaac
      721 agcgaagccg cggtgttcgc cgcgcgcccg cacacgctgc gcattcacat ggatgcggac
      781 ccggggcggc tgacgccggt cgtcgaccca tcgctctggg ccacgcgcat cacggtcggc
      841 acgatcttcg agccgctact tcgatacgta cctcctgatg cgcagggacc cgcgcgctac
      901 accgcgcgcc tcgcccgcag ctggcgcgtg atgccgagcg ggctcgagat ccggatcgag
      961 ctgcagcccg gcgtgacgtt ccacgacggc cgcccgctca cgacctcgga cgtgcagttc
     1021 acgctcgacg cgatgcgcac gccgcacaag ggcgccgatc acctgcgcgc gatgctcgat
     1081 gacgtcgaag cagtcgagct gatcacgccg ctcgagatcc gcctgcgcct caagcgaccg
     1141 agcagcatcg tgctgcgcgc gctcgccgag atcccgatcc tgccgatggc gatctacgac
     1201 ggcagcttgc tcgcgggcgg cgcgctcgtc ggtaccgggc cgtggaagtt cggctcgaac
     1261 aagaacggca tcgttcatct cgtgcgcaac gacaagtact gggacggcac gccgccgatc
     1321 gcggacgtcg agttcgtggt cgagcgcgat gcggccgtcg cgctcactgc cgccaaacgc
     1381 ggcgagctcg acatcattcc cgcgctcatc ccggcgcact ggcccgagca ggcgagcgca
     1441 cctggtctcg ccgcatcgtt cgtgccgctg cagctcgcgc caccgcgcct gcgctacttc
     1501 cagttcaatg ctgcacggcc gctgatcgcc gacgcgcgcg tacgtcacgc gctcgctctg
     1561 ctcatcgatc gccgtggcat ctcgaagcgc gtgttcgacg gcctcgcgcg tccggtcaac
     1621 tggccgatct ggccgggcgg ccctgtcgac ggcgccgagg ccccggtgcc cgatttcgat
     1681 cccgccgcgg ccggcaagct gctcgacgcc gccggctggg tcgacaccga caaggatggc
     1741 attcgcgatc aaggcgaggg caagcagctc aagctcgtgc tctacggcgt cgagacgccg
     1801 aaagtgcacg acgcctcggg cccgccggcc aagaccgagc gcgattattt cgtagaagcc
     1861 gcgcgccgcg ccggcgtcgt gatcgaggtg aagaccggcg gcgagtcgtg gctcgacaag
     1921 aagctcggcg aagggcagtg ggacatcgtc gagatcgcgt ggaacggccg cgtcgacatg
     1981 gacatcacgc cgctcgtcgc gggcaggacg ccgatccggc cggtgcagcc gcgcgtcgat
     2041 cacgcgctcg atgcgatggc cgaggcatgg gatcccgccg agcgcggcaa gctcgccaag
     2101 gagctcgccg acgctcttgc cgaaagctgg ccgatcgcgg gcatcgtcgc cgatgcgcca
     2161 caaggcctcg cgcatcgtcg attgcagaac gtgcgcgtgt ggaacgggtg ggtagattta
     2221 agccaactga agtttgtcga cgagagcaaa ccaagctctc cgtgattcgg tagctgacga
     2281 aacggggtct gaccggtctg accccgtttc tgcagggact gacccctttt ctggttacag
     2341 cgagcgcagc gagatgccgt attcgatttc ggtgcgcgtc tcggcggtcg ggaacgtcca
     2401 cttcgatacg gccgacgtga tgcacttgtg cgcgccggcc ggcacgtcgc cgccgagctc
     2461 gatgtcggtg accacaccgg cgtcggagat cgacagccgc agctccgcag tgcacgcatc
     2521 ggcgcggtgc gcctttggca gcagctccca gcaattgtgg atgtcgccca tatgcgcctg
     2581 gacgaccgtc gcgacctgcg cctggctcaa gctcttggga acgaacacct cggtgtcgcg
     2641 ctcggacacc ttcgtggcct gacgaccgcc aagcacgtag tccttgccca tgttgagcgt
     2701 gggcgccttc ggcttggccg ccgctttcgc cagcggcttc gcggttacga gcggacggcg
     2761 ctcgaggtcg gcgtacggcg cgagcacaga cgacgtgtcc gcgggcagga ccgggatgtc
     2821 gagcacctcg ggcaggggca gcgcagggcc cgtcgcgagt gcggcgccga gcgatcccgg
     2881 catggcggcg gcgccgagcg atcccggcat ggcggcggcg ctcgcggtag ccggcgctgc
     2941 gactgcgtcg gcgatcggct tcgtcgcatc gagcttcgcg tcgagcttcg catcgggctt
     3001 cgtgtcggcc ttcggcgcgt gggtcttctc ggcgacgagc gtcggcttgg tgtcgcggtg
     3061 gttcttcttg ttgttccccg cgaccgcagg cgcgctgaac actccgatgg tggtggcgat
     3121 ggcgaggaag gtcttcatgg ctgacgcccc caagagcagc cggcgtgcca cgcgtgatcg
     3181 cgaatcggcg agcacacttc cgcgagtcgc tggagtcggc cggccttggg tctttcgttc
     3241 cgcgggcgga agctcgagca gccactaacg agccactacc tgcgcgtcgt gccgacgagg
     3301 acaaccaacc ccagtggctg caacagcagc agcacgagcg cagcgagcgt gcccgcgtga
     3361 tccgcgacgg tgccgacgag aaacggcaac gccagcgcga gcggcgtgaa cagatggccg
     3421 gccgctagca cggcgcccga tgcgtcgggc tggcgtgcgt aggcctgcgc agccgcgagt
     3481 gggtagagcg gggcgacgca cgcgccgacc gggaccatga gcagcatcga gaggagcggc
     3541 gtcggtgccg cgagccaagg cacgtagctg accgcacagc cgatcgcact cgcagccagc
     3601 acccagcgct ccgatcgaaa cttcagtaga cgatcacaag cgacaaggcc gagcgcgccg
     3661 ccgatcacga gtgccgcgac aattgttttc gcggttctgc ggtgcctcgg aagaggtctt
     3721 aggtcgcgat aaagcgctcg tagatcgcga agccgagcgc cgcggccgac agtacgtaca
     3781 gcaggatcac gagaccaagg ccgccccgcc gggaggcggt caacggcatc gactgcgatg
     3841 cgcgctgcgg cactggggct ttcggtgcca ctgcgcgtgg cggcgtggcc ggcgcgtcga
     3901 gggtgccggg accgatgatg atcttggcgc cgctgtcgac cgggcgatcg tccttggcgg
     3961 ggtccttgtc accctcgccg gcggcagact cgccttgttc actcgtgcgc agggcgccca
     4021 cgcgtggatg gtacaattca tttttggatg gcgcacctcc tgctaatcga cgatgacgcg
     4081 cacggggacg cggtcgtcaa agcgctgagc aaagatggcc acgaggtccg ctgggtacgg
     4141 acgatggccg acggcttgaa gcagtttcgc gctgatcgcg cggatgtcgt gctcgtcgat
     4201 accgacctgc cggacgggtc gggacacgac gtgctcggcc aggtgcggat cgaagatccg
     4261 acgacgatgg tgatcatgac cagcgcgcgc ggcacgatcg aagaagccgt cgatgcgatg
     4321 aagcagggcg cgagcgactt cgtgcgcaag ccgctcgacc tcgccgattt gctgctccgc
     4381 gtatcacgca gcttgtcgaa tcgccggctc gcgacccagc tcgagtatct caaggccagg
     4441 caggcgcgcg gctcgaacat cgcaggcctc gtcggcgaat gccccgagat gcgcagcgtg
     4501 ttcggtaccg tgatccggct tgcccagaag acgggtcggc gcgcgggtcc ggtcgtgctg
     4561 atcgtcggcg agaccggcac cggcaagggc gtgatggcgc gcgcgctcca ctacaacagc
     4621 tcgcgtcgcg acggtccgtt cgtcgaggtg aactgcgcgt cgattcccga gagcttgatg
     4681 gaagccgaag tgttcggcgc cgagcgcggt gcgtacaccg gcgcggtcgc gtcgcacgtc
     4741 gggtacgtcg agaccgccga gggcggcacg ttatttctcg acgagatcgg gtgcctatcg
     4801 ctgccgttgc aagcaaagct gctcaccgtg ctcgagtcgc gcacgttccg gcgcgtcggc
     4861 agctcggtcg agcggcaagc ggacatccag tgcgtcgtcg cgacgaacat ggatctgcgc
     4921 gcggccgtcg agcgcggcgc gtttcgcgaa gacctcttgc atcgtctcga agtgatcgtc
     4981 ctgcgcatgc cgccgcttcg cgatcgcgga ccggatcgca tgcgactcgc ggggcatctg
     5041 cgcgacgaga tctgtcgcga gtacggattg tcgccgcggc cattcgacga cgacgtcgac
     5101 gacctggtcg acgactatca ctggcccggc aacgtgcgcg agctgcggaa ctgcctcgag
     5161 cagatcctgc tgctcgaaga cgagccggtg atccgcgcgc gacacttcat gctgcgcggg
     5221 cggccgaaac gctcgagcaa ttcgcgtctc ggcagcgagg gcggcggcat cgagctcgag
     5281 atgccggaag agggattggc actgcaggca gtcgaggacg cgctcatcga ccgcacgctc
     5341 gagaagtgcg acggcaacgt gtctcgggca gcccgcatgc tgggcgtcag ccgcgaccag
     5401 ctccgctacc ggctcgcgaa gcgcggaagc tagctaagag ctggaacctg ttggtactat
     5461 gacgccaatg accgggtggg tgagaatccc cgaatgggtg atctcaccca aaatctttcg
     5521 tgtgctaggc aaacaatcta gcagcttgtg atggagcggt tttcgcaagg acagaaacga
     5581 cggtggtgcg cagaaagtac tgtgtagcct gcgggagcat ctatccgcag gagctgcgag
     5641 cgtgtccgca agacggctcg ccgctcgcgt tcgagaacac gggccaccgc ctgcagctcg
     5701 gtcacgtgct cggcaactac cgcctgattc gcaagctcgg cgaaggcggt gtcggcaccg
     5761 tctacgaagg cgagcacgtg cggcttggcc gcaagatggc gctcaaggtc ctgcaccccg
     5821 agacggcgag cgccgagacg atcgtgcggt tcttcaacga agcgcgtgcg gtcaacgaga
     5881 tccggcaccc gaacatcgtc gatgtcgaag acttcgtgac gaccgacgac ggcgagcact
     5941 acctgatcat ggagctgctc aagggcgagg acctgcgcgg cctgatcagc cgcgaaggcg
     6001 tgatggatcc gcagcgcgtg tccgcgatcg gcgagcaaat cgcgcgcgcg ctcggcgcga
     6061 tccaccgcgc gggcatcctg catcgcgatc tcaagcccga caacatcttc ctcatccgca
     6121 aacccgatgg cagcgaggtc gccaagctcc tcgatttcgg gatcgcgaag ttcctcaccg
     6181 acaaggaagg catgactcgc gcgggcatga cgctcggcac gcccgagtac atggcgcccg
     6241 agcagatcgt gtcgagcggc aagcccgggc ctggcgccga catctattcg ctcggcatgc
     6301 tgatgtacga gtgcttggcg ggcgcgccgg cgttcactgc gacgacgacg gccgcgatcc
     6361 tgcgcggtca tatcagcgag ccgatcgtgc cgccgtcgca tcgtcgaggt gaaccgctgc
     6421 cgccggtgct cgagagcgca gtgctcaaat gcctcgagaa agagagcgcc catcggttcg
     6481 ccgacggcga tgcactcgcg gcggcattgc gcagcgatcg cgccgtcgag ctcagcacgc
     6541 cgcgctacac gccgggcaag cgacagggcc ggacactgca gatgttgccc gcgttcgcga
     6601 tggcagctgc agcggcggtc attcacttct tgccgcgcga cgatcgtgca gccgcggccc
     6661 cggcgccagt cgtcgcgatg accgcagtcc cgccgagcca gccagcgccg ccggcgctcg
     6721 accccgtgcc gcccccggcg ccagccgtcg aagaagaacc cgcggtgccc gcgccgacgc
     6781 acatcgaggt cacactggtc tcgaacccag aaggcgcgca gctcttcctc ggcaagcaag
     6841 cgcttggcga atctcccgcg gtcgcgacca tcccgctcgg caaccagccg gtgaccctcg
     6901 tggcgcgctt ttccgacgga gccgaggtga cgcagacgat cgtacccgac cggccgatcg
     6961 cgcaaattgc gttcgtgaag ccgcagaagc agcaatctgc tgcacctccg ccaccgccaa
     7021 gcgtcaagca aaagaccacg acgcaaaaac cgcgtgcgtc gtcgggtgac aaactcgaga
     7081 gccgcgatgg caccatggat ccattcgcca aatgaagtcg tgtcctacat gcgctcgcct
     7141 gtacatcgag agcgaggaca cgtgcgcgct cgataccgcg cgactcgtcg agaaaaccgt
     7201 gcgggcgccg gctggcgtgg ggcgcctcct cggctcgtat cagctcgtgt gtctgctcgg
     7261 cgagggcggc atgggcaaca tctacgtcgg tcgccatctg cagctccatc gctacgtcgc
     7321 gatcaaggtg ttgcgcgagg agctcgcgaa gcgcaaggag aacgtcgcgc ggttctttca
     7381 cgaagcgcac acgatcaatc ggctgcacca tccgaacatc atcgagtcga tcgatctcgt
     7441 cgaagacggc ggcgaaggcg cgtacgtcgt gctcgagctg ctcaagggac acgacctcaa
     7501 gacgcggctc gcgcacggcg cgctgccgat cgagtccgcg attcacatcg gcgtgcagct
     7561 cgccgacgcg ctcggcaaag tgcacgcgct cggtgtcgtc caccgcgatc tcaagcccga
     7621 gaatttgatc ctgctcgacc gcgacggccg cgatgacttc gtgaagctca tcgacttcgg
     7681 catcgcgcag atcggctttc acggtgacgg cggcatcgtc gggacggccg cgtacatggc
     7741 acccgaacag ggcatcgcga atgcgccggt cgatcagcgc gccgacatct actcgctcgg
     7801 cgtgctgctc ttcgagatgg taaccgggca gcacccgttc ccgtcggcga ccgacagcga
     7861 gtacatggtc cagcacgccg atacggcgcc gccgaagcca tcgaagctgg caccgcgctg
     7921 tccgcccgcg ctcgaggccg tgatcctgcg ctgcctcgcg aagtcgcccg ccgagcggta
     7981 cggcagtgcc gatcaagtcg cggcggcgct gcgcatggtc gatccacgca aggcaaacgg
     8041 ctcgcgtgcg gcctgggtcg cgtttggcct cgcgttcgtc ggtggtgccg ctgcggccgc
     8101 gtacttcttg ctgccgccgt acctcgcgac gtcgactgcc gaggcgaagg cggacgtcgc
     8161 gaccgtcgcg caagccgagc cggccgagcc gcagcacgcc gtggccgcgc cacccgcgcc
     8221 cgcgcaggaa gcacctgcac cggccccacc acctccgccg gcggcgcctg cgatcgtcga
     8281 gatcgggatc gtgtcgaccc cggcgggcgc atcggtgtat cgcaccggcg agacggtcgc
     8341 gctcggcgtc acgccgttca ccgtgccgct gtcgcggtcg gacaagccgg tgcagatgcg
     8401 cttcgagaag gccggctttc aaccgaagac aatcgaggtg ccgatcgcgg catcactcga
     8461 gatcgaagtt tcgctggcga agcagaaaca gccagcttcg aaggccactg tgcaaattcg
     8521 aaagccggcg gcgaagccgg tcaccgacac gaagcccaag ccgattcaac gcgagggcac
     8581 gatcgatccg ttcgcgaagt aagaggtgct catgcgagct ttgcttgcgt ccacgatgct
     8641 catgtacgtg ctggggggtg gcgccgcgcc tgcggcggcc gagaccgatc tgctcgtgct
     8701 gccggtgggc aaggacgcca aggagctgaa gccggtgctc gcgcgcgtcg gcacggtatc
     8761 gccgaaaccg atcgatgcca gctgtgcgag tgatccgggc tgcttgaaca aggccggcgc
     8821 cgacgcgggc gcgcatcgcg tgctctcgat taccgcgagc ggtacgcgct acacgctgat
     8881 gctcgtcgat gcgagcacca agctcctgct cgcgacccgc gatatcgacg ttccgaagaa
     8941 gcggctcgcg aaggacctcg gccccgtgct cagcaagttc gtcgacgacg cggtcgtcga
     9001 caaagcaaag gcgctgttcg ccgagggcaa cgagcactac aacctcggcg agttcggacc
     9061 tgcgctcgag cgctacaagc tcgcgtatcg cgtcaagcca ttgcccgcgt tccagttcaa
     9121 tatcgcgcag tgccatcgca agctcggcca gtatcccgac gcgatcgcga tgtatcaggc
     9181 gtatctcgtc ggcgtgccga gcgcacagaa caaggcaacg gtcgagtcgc tgatcaccga
     9241 gacgaagaag ctgctcgccg atcagcaagc gctcgagcgc cagcgcgaga gcgagagact
     9301 cgccgccgag cagaagaaag ccgaggaggc gcgtaaggca accgaggccc aggccgctgc
     9361 cgacgctgag accgcgaagg ccgagcaagc ccgcgtcgcg gcggagcgtg cgcgcgacgc
     9421 gacgtacaac cggcatccgg cgcgcggctg gatgctgctg accaccggac tcgggctcgc
     9481 gaccggcggc gcaggcgcgt atttcgcggt gcaagcgcgc gacgagcagc gcaagttcga
     9541 cgcgctcgac tgcggcgatc cgatcaagaa gccgctgctc gacatggcga cgctcgatca
     9601 gtgcaccgac catcagaaga agggcgatcg cgatgcgttc ctgacgaacg tgctgatcgg
     9661 cagcggcggc gcgatcgtgc tcgcgtcgct gatcgtgttc atcgcggacc cgggcaacgt
     9721 cgagcggccg aaccgcatga acgtctcggt gacccacaat tccatcgacc tggtggtgcg
     9781 atggtagtca tcacgagagc gctgctcgcg ctgatgctcg tcggggttgc ggcgtgtcgc
     9841 ttcgactcga aggagttcga ggatcgcagc tgcacgcgcg acaccgactg ccgccccgat
     9901 cagagctgcg tcgcgggact ctgcacgcag aagacgtgcg ccgcgagcac cgattgcggc
     9961 cctgacaaca cattccaatg caccgacggc aagtgctacg ccgatgcatg cagcgatgac
    10021 tcggcatgcg gcccgggcta cgggtgtgaa accgggttct gccacgtggc gtgcgtcgat
    10081 cacgacggcg acggcatccc gttcggaacg ccgtgcaccg gcacgcagga ctgcgatgac
    10141 ctcgatccaa cggtcacgcc gggaacggtc gagggcatgt acggcgacgc gatctgcagc
    10201 gacggcaagg acaacgactg cgacggcatc aaggacggtg ccgagctgac gtgtggcgct
    10261 cagtgcgcga acggcgatga tgacgacgac gacggcacgc tcgacttccc cgacgacccg
    10321 ggctgttcgt ccatcgacga caacagcgag aagggcaccg ccatctgcga cgacggcgtc
    10381 gacaacgaca acgacggcaa gctcgactac cgcaccgacg gtacgggcga tcctggctgc
    10441 atggcgccga ccggcgcgag cgagctgtcc aacactgcgt gcaacgacgg catcgacaac
    10501 gacatggacg gcacgatcga ctaccgcaag gatggcaccg gcgatccggg ctgcgtcgac
    10561 gacgccgaca cgagcgagcg cggcacgacc gcgtgtgacg acggcatcga cgacgacaac
    10621 gacggcacga ccgactaccg gcagggcggt ggcggcgatc caggttgcac cgatccgatg
    10681 gacatgaacg agcgcggcac gaccgcctgc gacaacgaca tggacgacga catggacggt
    10741 cgcaaggact tcaacttgaa ccccgccgtc ggcgacgcag gttgcagtgg cccgaccgac
    10801 aacgacgagc acagcactgc ggcatgcgac gacggcatgg acaacgacaa cgacggcacg
    10861 accgactggc gcgccgccgg tggcgatccg ggctgcgcgg atctcgccga caacagcgag
    10921 cgcggcaccg gcgcgtgcga caacggcctc gacgacgacg gcgatggccg cacggactac
    10981 aacctcacgg cgtcgctcac cgatccgggc tgcagtgatc cgaacgacgc gaacgagaag
    11041 tgcagcggca tcggctgccc agcgtgtgac aacggcgtcg acgacgatgc ggacacgcgc
    11101 accgacttct cgacgacgat ggcgatcagc gatcctggct gctcgtcgcc cgcggacacc
    11161 gacgagaaat gcagcggtgg cacctgtccg cagtgcgacg acaacatgga taacgacacc
    11221 gacacactgt tcgatctcgc cgatcctggc tgcgcgggta gcccgaccgg gaacaacgaa
    11281 gcgagtgtga gcgccgcgtg cgcagacggc gtcgacaacg acggcgacgg ccgcaccgac
    11341 ttcggcgcgg ggccgacgaa cgatcctggc tgctcgagcg cgaccgatac gaacgagcac
    11401 gacagctcgc tcgtgtgcga cgacggcatc gacaatgaca acgatggccg ctccgacttc
    11461 aacgcgacgc cgggcgccgg cgatccaggc tgcgcgagcg tgaccgatac gagcgagaag
    11521 ggcgcgacgc tctgcgacga cggcatggac aacgacaacg acggccagat cgacttctcc
    11581 gtcgctggcg cggacccggg ttgcaccggt ccagcggaca acaacgagca cgagaatacg
    11641 ttgcgctgcg acgatggcac cgataacgac ggcgacggca ccgcggactt ccagctcagt
    11701 ggcggcgacg ccggttgctc gtcgctgcag gactcgaacg agcgcgggaa taccgcatgc
    11761 gacaacggcg tcgacgacga cgccgatggc cgcaccgact tcaacgtgat ggcggcgctc
    11821 accgatccgg gctgttcatc gccgaccgac tcgagcgaga aggggacgac cgcgtgcgac
    11881 aacagcgccg acgacgacgc cgacggtctc accgacttct cgacgaattc ggcgatcagc
    11941 gatccgggtt gctccggccc ggatgacacg aacgagaagt gcacgcctgg ctccggctgc
    12001 ccgcagtgcg acgacggcag cgacaacgac gccgacacgt tggtcgacct cgcagacccg
    12061 ggttgcagcg gttcgccgaa cggcaacaac gaagcgagtg tgagcccggc atgcgccgac
    12121 ggcgtcgata acgacggcga tggccgcatc gacgtcggcg acgacccggg ttgtacgagc
    12181 gcgacggatt cgagtgagaa gggcaccgac gtatgcgacg acggcgccga caacgacacc
    12241 gacggtcgca ccgactacaa cgtcaactcg gcgatcggcg atccgggctg cagcgcgccc
    12301 gacgatccga ccgagaagtg cagtggtggc ggctgcccgc agtgcgacga caacaccgac
    12361 aacgacaacg acggcaagtc cgattacacg ctcgggggcg cggatcctgg ctgcagcggg
    12421 ccgaccgaca tcagcgagca cgacgcagcg ctcgtctgcg atgacggtat cgacaacgac
    12481 aaggacggtg tcagcgactt ccagctcagt ggcggcgatc ctggctgttc gtcgctatcc
    12541 gacagcagcg agcgcggcac cgcgacgtgc gacgacggcc tcgacaacga taacgacggt
    12601 cgcatcgact acaacatcag cgcggtgctg tcggatccgg gctgcacgga tccgacggac
    12661 accagcgaga agtgcaccgg cgtcggctgc cctgcgtgcg acaacggcgc cgacgacgac
    12721 gccgacacgc gcaccgacta ctcgacgaac ccggcgagct ccgaccccgg ctgcagcgga
    12781 cccgacgaca ccggcgagca ttgcagcggc gtgggctgcc cgcagtgcga cgacgatatc
    12841 gacaacgacg ccgatacgct caaggatctc gccgacccag gctgcgcggg taacccgacc
    12901 ggcaacaacg aagcgagcgt gtcaccggcg tgcgccgacg gtatcgacaa cgacggcgac
    12961 ggccagaccg acagcaacga tcccggctgc gcgagcgcga ccgatccgag cgagaagggc
    13021 accacgctgt gcgacgacgg cgtcgacaac gacctcgacg gccgcaccga cttcaacgtc
    13081 aatgccgcgg tcagcgatcc gggctgcagc gcgcccgccg atccgaccga gaagtgcagc
    13141 ggtagcggct gtccgcagtg cgatgacaac accgacaacg acaacgacgg ccaggccgac
    13201 tacacgctcg gcggcgccga ccccggctgc agcggaccga ccgacacgac cgagaagagc
    13261 acggcgccct gcgatgacgg cgtcgacaac gatctcgacg gcctgttcga cttccagctg
    13321 agcggcggtg atcccggctg tgcgtcgctc accgatccga gcgagcgtgg caccgtcgtg
    13381 tgcgacgacg gtatcgacaa cgacaaagat ggcctgatcg acttcgcgac gaacggtggc
    13441 gatccgggct gctcgagcgt cgccgacacg agcgagcgcg gtgtgcttgc gtgcgacgac
    13501 ggcatggaca acgacctcga tggtcgtacc gactacaacc tcgatggcac gcagtccgat
    13561 ccaggttgca ccggaccagc cgacaacaac gagaagggac cagcgtcgct ggtctgcgac
    13621 aacggcatcg acgacgacac cgacggccgc accgattacc gcaccgacag cacgggggag
    13681 tcgtgttgta cgtcgccgac tggcacgacc gagcaatgcg cgtgcgtcgc cgatctcgac
    13741 ggcggcaccg tgcacacatg cgcggcgaag acgagcggtg cggcgttctg ttggggcgac
    13801 aatgcgaacg gcgcgctggg cgacggctcg acgacggatc ggcagacgcc ggtccaggtc
    13861 ttggtctcga taggcacgcc gctcacgggt gtcgtccatg tcgcgaccgg cgatttgcac
    13921 tcgtgcgcgc tgaagagcga cggcacggtg tggtgcttcg gccgtaacaa cgcgggtcag
    13981 atcggcgaca tgacgaacat ggcgcgctcg ttcgcgaccc ccgtgatgaa cctgaacggc
    14041 gtgatcgcga tcgcggccgg ctgggatcac agctgtgcgg tcaagtccga cggcaccgtg
    14101 tggtgctggg gcgcgaacgg caacggtcag ctcgggaacg gcttgtccgg aaacatgaac
    14161 agcagcaacg tgccggtgca ggtcatgggg ctcaccggcg cgacgcaagt caccgccggc
    14221 gagctccaca cgtgcgcgcg caagagcgac ggtactgcgt ggtgctgggg ccgcagcaac
    14281 aagggccagc ttggcaacga cacgatgatg gacagcaacg tgcccgtgca ggtgaagtac
    14341 ttccagggca cggtgaagaa cctcaccggc gtcaccgagg tgtcggcggg acagacgcac
    14401 agctgcgcgc gtctcggcac gaccgaggta cgctgctggg gcctcaacgg caacggccag
    14461 ctcggcgaca attcgatgac ggacagcgcg cgcgccgtgc aggtcagcaa cctggcaaac
    14521 ccggcttcga tcgcggcggg cggctcgcac acgtgcgcga tgctgacgac cggcaccgag
    14581 gtgtgctggg gcttcaacat ggcgggtcag ctcggcaacg gcatgacgat gacgagcctc
    14641 gtgccggtac ctgtgaacac gctgacgaag atcgtcacga tcggcaccgg cgcctcgcac
    14701 agctgtgccg cactcgacac cgaaaccaca caatgctggg gcagcgacag catgggccag
    14761 ctcggcaacg gcaacggcgg tagctcgtcg agcccgggcg cggtgaccct gacctgtccg
    14821 tagttacggt gcgggatcga gcgggggtgc cgtgggcgtc gggggtggtt ccgcgggcgc
    14881 agcgtcgggt ggcggttcgg cgggctttgc cacggcgggt ttcgccggcg gcggtttggg
    14941 tttgcgacgg atcggcttgg ggtgcggcgg cttcgcgggt gctttgacgg ccggcgcggt
    15001 gccggcgtcg atgacgggca ccgtggcagc gcccgcatcg atcggtggtt ccgccacagg
    15061 ctcgctgatc gcggcatcga cggcctcgac ggcctcgacg ctcgcggcgt cgagcgcggg
    15121 ctcggtgctc ggcgtcgcgg ctgcgtcctg gcgttggggt gctgcggccg gcgtcgaacg
    15181 cgcgccgagg tcctgataga ggaagaagta tccggcggcg agaccacccg cgagcagcaa
    15241 gaagacgacc agccacaccc acgggaacgg tgagcgtggc gcgactggat agacgacatc
    15301 ggggaccacg gggccggagg gctcgccgtg ggcgatcggc ttcgcggcct tgatcgcttc
    15361 gagctcgggc ttcgtcgaat cgtcgcgctt gggcttcgtc ggctcgatcg cggcggccgg
    15421 cagtggcgcc gacggctcga gcgccgcgac caccgcgacc gccgcgcgct cgatcgccgc
    15481 cgcctgcttc gcatccgcca cgacgtcgag ctcgggcggc tcgggcgcgg gcgggggaag
    15541 cggcgttgca tcgaacgttg gctgcgacgc gcgcgagacc ggaatgagct gcgccgatgc
    15601 gcgaatgcgc tcgaggacct ccgccgaggg tggtggcacg gcctcgtcga cgagccacgg
    15661 ctcgagcacg tcaccaaact gctccttcat gtagtcggac agccggcccg gcgaagcggt
    15721 gagtccggcg acgcgcgcga atcggtcgag cgtctggcgc agctcgtcgg cggtctggta
    15781 gcgctcggcc ggattgttcg cgagcgccct catgatgatc ttctcgaggt cgtcgggcag
    15841 atctttgcga aaccgcgacg gcggcggcac gttgccatcg acgatcagcg acatcgtctc
    15901 gtagcgcgta tcggccttga acagccggcg caccgtgcac agctcgtaga gcacgatgcc
    15961 gagcaggaac acgtcggtcc ggcgatcgag cggctgcgcc cggcattgct cgggcgacat
    16021 gtagccgagc ttgcccttga gctcgccgcc ctgggtgtcg tgcgcgcgca cgtgtgtctt
    16081 cgcgatgccg aagtcgacga cctttacgcc gccgtcgaac ccgatgagga tgttgccggg
    16141 cgaaatgtcg cgatggacga tcccgagcgg ttggcgatcg ggtccgcgtt gctcgtgggc
    16201 gtagtgaagg ccggccgccg cggcgctcac gatcgtgatg acgtgctcga gcgggatctg
    16261 ctctttcttg tctttgacgt ggcggagtaa ctcccggacg tcgcgtccgt gaacgtactc
    16321 catgacgaag aagtacttgc cgtcctcctc gccgacgtcg tggacttgca cgatgttgcc
    16381 gtgatggagc gccgcaacca gtcgcgcttc atcgaggaac atcgccatcg atttctcgtc
    16441 actaccgtgt tcgacgcgga ttcgtttgat gacgacgtga cgggcaaagc cggcaaggcc
    16501 aatcgcacgc gcgagcaaca cctcggccat gccacctttc gcaaggggct tgagcacttg
    16561 gtaccgccct agcatcgtcc ccgtcgctgg agccgtcgtt cccaactgcc caagcgtcgc
    16621 aaccgaggga gggcagcgtc aagggcacca tcgcgctccg tcccgcgcta cacatacggc
    16681 atggccacac ccgcgatcgc cggcaccggg gtgcgtgcgg cgctcgacgc gacgttcgca
    16741 gcgctgccgg cccagttgcg cgtgccaggc accggtggcg ccatttgtcg gcagattgct
    16801 gacgtctatg atcacgcgct tgctgctcat ttcgcgcagg ccaccgcagg gatcggcgag
    16861 cccctcgcgc tggtcgcgac cggcgggtgg gcgcgccgcg agctggcgcc gtactcggat
    16921 atcgacttca tcgtcctcca cgatcgcgac gaggccaccg cgaagcaggt ctccgaccgg
    16981 ttgctctatc cgttgtggga cgaaaagctc gcgatcggcc acgcgatccg ggagccgcgc
    17041 gcggcggccc ggctcgcccg tggcgacctc gcgaccgcca ccgcactgct cgacgcccgt
    17101 cacatcgcgg gcgaccgccg gcttacgaac gagctcgtgc gcgcgacgct atccgtgctg
    17161 tcgccgggcg gcaatccgaa cgacctcatc acgttgctcg ccaccgagaa gaaggcgcgc
    17221 cacgatcggt tcggcgcctc gctctatctg ctcgagccga atctgaaaca aggcatcggc
    17281 gcgctgcgcg atctgtcgac cgcgctgtgg gcggcgcagg tgcgctggca cccgccgcgg
    17341 ccgggcttcg atccggagag cgccgaatcg ctgatcacga acctcgtcaa gctgggtcac
    17401 ctgacccggc gccagggcga cgtgctctcg ttcgcacgcg acttcttgct gcgtattcgc
    17461 gtcctcgtcc agctgaccgc gaagcggcgc ttcgatcagc tcacgttcga gatccaggaa
    17521 gcggtcgcgc cgagcctgta tcccgacgcg cggccgcacg agggcgacat tcgctcggcg
    17581 gtcgcgccgg ccgtcgaggc gctgatgcgc gactactact tgcacgcgcg tggcgtcgtg
    17641 caggttgccg acctgctgct cgaatcggcg cgagtgccgg cgcgccgcaa gccgcgcatc
    17701 gccgagatcg atcaaagctt catcacgttc aacggcgagc tcgcgatcaa ggatccgcga
    17761 ctgtttgccg agcggccgag cgagatggtg cggatgttcc gcgttgccgt cgatcagaag
    17821 ctcccggtct acggccacac ccgcgagctc gtcgccgaga cgatcgcgcg cgatccggtg
    17881 ccgctccagc gcgatccact tgccgcgcgc atgctgctcg acgcgctctc cgatccgcgt
    17941 gatagcgcgc agccgtcggc gttcgaggtg atgcaccagc tcggcatcct gtcggcgctg
    18001 atgccggaat gggcaccgtg caccggtcgc gtccagcacg acctctatca cgtctacacg
    18061 gtcgaccagc accagctgta cgcgctcgca atgcagaagc gactcgcgcg cggcgagctg
    18121 aacgaagagc atccgtacgc gaccgagctc tggcgcgagg tgaagcgacc ggccgcgctg
    18181 ttcctcggca cgctgctgca cgacgtcggc aagccgctcg gcaaaggcca cgccgagaaa
    18241 ggcgcggtcg tcgccgctgc gatcgcaaag cgcttcggga tgaccgacga cgagatcgag
    18301 gtcaccgagt tcctcgttcg ccagcacctg acgatggcgc acctgtcgca gcgccgcgat
    18361 ctcaccgatc cggacacgat cgcgcggttc gccgagcgcg tcggcagcga ggatcatctc
    18421 atcaagctct atctgctcac gctctgcgat acggcgatga cgaacccgga gaacctcaac
    18481 gcgtggaagg cggggctctt gagcgagctc gcgattcgca cgcgccggta cttccgcggc
    18541 gagtcgctgc gcggtgagtc ggccgatacc gagaccgagg acggcgagcg cgcggcacgc
    18601 gagaagatcc tggcgatcgc gagcggcgat ggtgcgctcg ataccgagct cgcacgcggg
    18661 ctcgtcggcg gtatcgatcc tcgcctgatt tcgcagctca ccgcgcgcca ggccgcgcgt
    18721 cacgtcaagc tcgtcgctgg cgcgcagcac aagcaaccgc ccgtcgggct cgaggtgcac
    18781 tgcttcccga tgcgcgggca tagcgaggtg tcgatcgtcg cgcccgatgc tcccggcgtg
    18841 ctcgccgcga tcgcaggagc gctcaccgcg aatcgcgtcg acgtgctcgg cgccttcctg
    18901 ggccacatcg atcttccgga tcgccgccgc ctcgtcatgg acacgttcta cgtccgcgac
    18961 ctcaagggcg ccgcgatcac cgacgacgac gcgcggtggg cgcgcctcgc caacgacctc
    19021 cgcgagctac tcggcggtgc gccggatccg ggcgcagtcg cgcagctgat ccagaagcgc
    19081 cggccgaaga gcggcatgcc gaagcggcac acgccgggcg tgatcaccga gatcaagctg
    19141 cacgacgatt cgtcgcactc gacgatcgtc gaagtgttca cgcaagaccg ggtcggtgtc
    19201 ctctacgcca tcacccagac gctcaccgat ctcggcctcg atatcgcgct cgcgaaagtc
    19261 tcgaccgaag gcgagaaggt cgccgacgcg ttttacgtcc accgtgccgg cgaacgcatc
    19321 accgatgcgc gcgaacgcga gacgctgatc tcccgtctgc gcactgcgct cgaggcgccg
    19381 ggcctgaccg gcggatgagc gtcgtcgatc tgccgcccgg catcgggctc gtcggtacgc
    19441 cgctcggcac gttcgtcgcg ccgcgccgcg cgcgccggct gtccgcgatc gcgctcatct
    19501 gcgtcgtcgc attcggtgcg ctgacgtggg cgttcgtatc gcgccatccg tatcgctggc
    19561 acgcgagcac gcagttcgtg ttgccgatcg cgttcgccgc gatcgcactg ctcgggttcc
    19621 tcgtgcgtcg cgcgagcgtt gcggtcacgc gcgatggtgt gcgctggggc tgggcatcgc
    19681 tcaatttcac gcaggaggcg tcgcgcatcg tgaccgcgca cgtctatcac gatggcatcg
    19741 cactcaaagc gaagcgcggg tcgtggtggt ttctgtccgc gcgcgactgg gaccggttcg
    19801 acgcgctcgt gcgccagcta cgccgcgccg agctgccgat caccgatcac gagggcaggg
    19861 cgccgttacg agcgcggctc cagagttacg gtcggttcct cgacgtgctg cttgtcggct
    19921 cgatgctcgc gacgttcgcc gtgatgctat gggcggcctg agcctctgac gtgcacgtac
    19981 acgtacacgt ggcacgtgca cgtgctcgtg ctttcgcttg ggcgtcgctg ctgatgacgt
    20041 cgcacctgta cgtcgtgttg atccggcaat gctcgctccg atgtgcgtcg atccggactg
    20101 accgtgtcct aagcacgctg ccggttcgag gcggtgcggc ttcgagaacg gctgccgtcc
    20161 gagcacgtct acgtgcacgt gacacgtgca cgtgacacgt gcacgacgat tctatgggcg
    20221 gccgagggct gtatagagct cggcacccgt gaagtcgcgc ttgccaccac gctcggcatc
    20281 cttgatcaag ccgaccagcc gcgcgttgac cggcgcctgc aggccgagcc gatcggcgag
    20341 cgccacgatc tcgccctgga tgtaatcgat ctcggtcggc cgcttcgcgt cgaggtcgtc
    20401 gcacatcgag gagcgcgcgt gcggatcgat cgcgacgatg cgcttgccga gcaactgaaa
    20461 gacgccgttg gggacggtca gcatcttcgc gatccacgtc gtgggcagcg gcgtgatgcg
    20521 ggcgagcggc tgctccgccg ccttcagaag tgcgagcgct tccctctggc aggccgcaag
    20581 acaccgccgg aagtcgcgat ccgagagctc ctcggcgagc ggcttgcccg agagcgcgtt
    20641 gatcgcgttg ttgagattga ggacgagctt cgaccactga acagcggcca tgtcgtcgcg
    20701 cagctcgaac gcgaggtccg ccttcaagca cgcctcggac agcggcgcga tcgcgtcgtt
    20761 cgcgtcgaac atcagcgtgc cggccgatgc gcgcagatag tagccggccg cgcggcgcac
    20821 gacgttgaac ggcaccatgc cggcgagcac gcgatgatcc ggcagtgcat cgcgcagcac
    20881 gcgaacgtta cgcacgccgt tctgcaagct gaccacgacg cagctcttcg cgaggtgcgg
    20941 tgcgagctcg cgcccggctt gtgccgtcgc agccgacttc acggtgacga gcacgaggtc
    21001 ggcggtcgcc agcgcgctcg cttccgtcgc gagcgttggc ttcgcttcac gtgtcccgcc
    21061 ggtgagatcg ctgaccttga ggccgttctc gagctcggcc atcacccgct gtcgcccgac
    21121 gagaatgaca tcgcctccta gccccgccgc tgacagcttg ccgcctaccc agcaaccaat
    21181 ggcgcccgca ccgaatacgc caatgcgtgt catgcgcgca caatactctg ctgggcataa
    21241 cggatgcagt tttcctcatg catgagcgat ttgattcaat cccctaagca agagtccggc
    21301 gctatcggct acatcctcgc gttcttgatc ggcattccga ttccgatcct cctcgcggtc
    21361 ttcttgatcc gcggctgcga ttgatcagaa gcgtccgcga ccgcgaatca cgacggtgtt
    21421 cgacgttcgc caccggccgt cgacccacac ataacggtcg ccttgccgaa cccaacgccc
    21481 cggcgcgtac acgtagccgg ggcgttcgcg ttcgtacgta ccgtcgaccc agtgccagcg
    21541 atggtggtcg cgcatccagt ggccttcgat ccatacgtag ccgggccgag ccgtcacgta
    21601 ctcttcgcgc ggcgccggcg gcggatcatc gccggcgacg atcaccgcgt gtcctgacgc
    21661 gccgcagcct ggcgctgctg ctgccaacgc agcgcaaagc aagctcgctg ttagcctcat
    21721 acgatcctcc gatctcgcag gatcagagga gcaagcgatg cgccgcaccg agtggcgcgc
    21781 accgtgcgtg cgctgctcag cgtttcgctg ctcagcgttt cgctgggcgc gggacgatgt
    21841 tcgtgatttc ttcttcgacg agatcagagg tgctgcgcgg tgcgcgcgaa cgcggcagcg
    21901 gttcgagcgc gggctggctc tgcgccagct gctgagtcac ctgtgcgagc aacgccgggt
    21961 ctgcgttgcg ttcgtcgatc acgaacgtgc gcgtcgctcc ctgcggctcg ttgggcttcg
    22021 tccgcatcct cgcggcgatc aaatccgcga gcggctcgag atccgcgacg tcgagctcgg
    22081 gctcgcggcc gagcgactgc agcgtttgcc gcggtgctct gcgcgtcccg gtcttggcca
    22141 tcgcatcgga gatgtagtcg tcgatcatct ctaggaggct gcggtcgaag tccattacgg
    22201 ctttgctccc tttgtgatcc acaacaggaa cagcttgtag tccgcgtcgt tcacgtcgag
    22261 gaacgttgcg ttggggtgat cttgcggcgt cggcggcgct tcgtaaagag gccgtttgag
    22321 caataacgaa tccgccggtg aagcggagtt cactacgccc gtcgccgcaa tgatgttcgc
    22381 gagcacggta ccggcggctt cgtcgtactt cagcacggcg gcaggacccg acaacgtgtg
    22441 gcagttcgcg cagccaagac caccggcggc ggcgcgctgc agcttcggat agatgtccgt
    22501 cgcaaacgac gggtccgtga cgttgttacc gccaccaccg ccgccaccgc cgcccatgcc
    22561 gccactgatc gcgagcgtgc cgccgtcggc gacggtcgtg accggcgtgt tgttcgtcgt
    22621 gttttgattc atgccgttca ggtacgtgac cgcgagcgtg tacgtgcccg gcggtacgtc
    22681 gaggatcgcg acgcgcgagc ggccttgata cgcagtcgca gtcgtcagcg cgagatcgac
    22741 gctgcccgcg gcgttgaaga agtacgggcc tttcacggta gcgacgggtt ggttctgcgc
    22801 atcgagcagc gtgacgttcg tcagcggaat gccctcgagc ggcgtgccgt cgttgcggcg
    22861 catctccgcg atgaggatcg cggtgccgag gaccggcgtc gcgccgaccg cggtgtactg
    22921 attcttgacg tcttgatcgg tcatcaaata gacgtccttg gtcaccggca tgtcggcgac
    22981 cgacatcgca gcgttgcgcg tcggccgata gttcgccttg ctggcgttga tgaacagcga
    23041 gctgccgacc gcgacgtcga gcgagtacgc gccgtcggct gcgctcgagg tcatgagcgg
    23101 aggatcgagg ccgtcggtcg cgaccatcgc gccttcgatc gcggtcgcgc cgaaatagtc
    23161 catgaccttg ccggacagga ggaagctcgt cggcgcggca tcgtcaccgc cgcccgccgc
    23221 atcgggccga tcggcgacat tcggcggcgc gctccccgga caaccagaga tcgctaccag
    23281 gaggaaaagg ctaagaagct tacgcatgac aggacctctt cacacgcgtg aaacatcaat
    23341 gactatgcca gggtaacgta gcgaaattgg gtctgtccga acgttgcact ctgggagtga
    23401 agcccccatg ggtgggggag gttgcaccga gaggcattgc cgggatcaga ccggtcatct
    23461 ataacgcgcc atgctcgagg aaccccttcg agaggcactg accttcgacg acgttctcct
    23521 ggtcccgtcg tttagcgaag tgatcccgcg cgacgtcgat gtcgccacgc gactgacgcc
    23581 tgcgattccg ctgcgcatcc cgctggtcag ctcggcgatg gacacggtca ccgaagcggc
    23641 caccgcgatc cgcatggcgc gtgaaggcgg catcggtttc atccacaaga atctgacgat
    23701 cgagcagcag gcgcgcgagg tccatcgcgt caagaaagcg cagtcgggca tcgtcgtcga
    23761 tccgttgacg atcgcaccat cgcgcacgct cgaagatgcg ctcgcgatca tgcgcgcgaa
    23821 ccgcatcagc ggcttgccgg tggtcgatgg cgatcgccgg ccggtcggca ttctcaccaa
    23881 ccgcgacgtt cgcttcgaga agcgcctcga cgtgcaggtc ggcgaggtga tgacgaagaa
    23941 tccggtcacc gtgaaggagg gcgtccagat cgacgaggcg aaggacctcc tccacaagca
    24001 ccgcatcgag aagctgctcg tcatcgacgg cagcggcaag ctcaagggcc tcatcacgat
    24061 ccgcgacatc gagcaggcac aagagcatcc gaccgcggtc gtcgatgatc tcggccgcct
    24121 gcgcgttggc gccgcagtcg gcgtcggccc cgatcgcgaa gcacgcatcg aagcgctcgt
    24181 cgcggccggc tgcgatctga tctgcgtcga caccgcgcac ggccactcgg ccggcgtgat
    24241 cgccgcggtg cgcgacatcc gcaagaagtt ccccaagctc cagctcgtcg ccggcaacgt
    24301 cgcgacggca gaagcgacgc gcgcgctgat cgagaccggc gtcaatgccg tcaaagtcgg
    24361 cgtcggtccc ggctcgatct gcaccacgcg catcgtcgcc ggtgtcggcg tgccgcaact
    24421 caccgcgatc gcggatgcgg tcggcgccgc gaagggctcc ggggtcgcga tcatcgccga
    24481 cggtggcatc aagtactccg gcgacgtcgc gaaggcgctc gccgcgggtg cggacaccgt
    24541 gatgatcggt tcgttgttcg ccggtaccga cgaggcgcca ggcgaggtca ttctctacca
    24601 gggccgcagc tacaagtcgt atcgcggcat gggctcgatc ggcgcgatgc aggaagggtc
    24661 gaaggaccgc tacttccagg ccgacgtcga agcgccgcag aagctcgtgc ccgaaggcat
    24721 cgaaggccgc gtgccgtaca agggcgcgct gcgcgagtcg ctctatcaac tgatcggcgg
    24781 cctccgctcg tcgatgggct atctcggctg cgcgacgatc gcggagctgc acgccaaagc
    24841 aaggttcgtg aagatctcgg catcggggct gcgcgagagt cacgtccacg acgtcatcat
    24901 tacgaaggaa gctccgaact atcgcgtgga gtagtccccc gacctcggtc gatcaagctc
    24961 aggcggtcga taagtcatgg cgcatcaact agttctggtc ctcgatttcg ggtcgcagta
    25021 cacgcagctg atcgcgcgcc ggattcgcga gcagtcggtc tattgcgaga ttcatccgta
    25081 cacgctcgca aatgatctgg cgaaggttcg cgcgctggcg ccgattggca tcgtgctctc
    25141 gggtggcccg aacagcgtct acgacgaggg cgcaccgtcg gttcccgccg ggattttcga
    25201 gctgggcatt ccggtgcttg gcgtttgcta cggcgcgcag ctgacggcgc tcgttctcgg
    25261 cggcaaggtg cgctcggcgg agcggcgcga gtacggccgc gcgaccgtgc gtgtactgaa
    25321 gggcgacgag ggtgtgttcc gcaccgtgca tgccggcgag gagctcgcgg tgtgggcgag
    25381 ccacggcgat cacgtcgacg cgattccgcc cgggttcgtg cacacggccg agagtgacaa
    25441 ctgcaagttc agcggcttcg tccatgccgc gcgcaagatc cactgcgtcc agttccatcc
    25501 cgaggtcgtg cacacgccgc gcggcgccga gctgtactcg aactttctgt tcggcgtctg
    25561 cggcgcgaag ggcgactggt cgatggcctc gtttgtcgac gaggcggtcg agcgcattcg
    25621 cgcgcaggtc ggtgacaatg gtcgtgtgat ctgcggcctg tcgggcggcg ttgattcgtc
    25681 agtggcagcc gcgctgatcc accgcgcgat cggcaaccgg ctcacctgca tcttcgtcga
    25741 caacggcgtg cttcgcaaag gcgagcgcga gcaggtcgcg gtcatcttcc gcgatcactt
    25801 ccatgtcgac ctccgcgtga tcgacgccga agagcgcttc ttgaaggcgc tggccgacgt
    25861 caccgatccg gagaagaagc gcaagatcat cggctacgag ttcatcgccg tgttcgagga
    25921 gcagtccaag agcatcgaga acgccaagtt cctcgcccag ggcacgctgt atcccgacgt
    25981 gatcgagagc gtgtcgttca agggcccgag ccacacgatc aagagccatc acaacgtcgg
    26041 cggtctgccc gagcgcatgc agctcgcgct cgtcgagccg ctgcgcgagc tgttcaagga
    26101 cgaggtccgc cagctcggcc tcgagcttgg cctgccgaag cacatggtgt ggcggcagcc
    26161 gttccctggg cctggcctcg cggtgcgctg cctcggcaat ctcactcgcg cgcgcctcga
    26221 cgttctgcgc aacgccgacg cgatcatcga ggaagagatt cgcgcggcgg gcctctacga
    26281 atcgatctgg cagtcgttcg gcgtcttgtt gccggtgcgc tcggtcggcg tgatgggcga
    26341 tgcccgcacg tacgaagagg cgctcgcgct gcgcgcggtg cattcgcgcg acggcatgac
    26401 ggcggactgg gtgcagttgc cgtacgacgt gctcggccgg atcagctcgc gcatcatcaa
    26461 cgaggtgcgc ggcatcaacc gcgtcgtcta cgacatcacg agcaagccgc ccggcacgat
    26521 cgagtgggag tagcaggctc gtgaacgagc tcgcactcca gcccggcgaa cggatcctca
    26581 agagcgacgg cgaggtgatc tggctcaagg gctggttcgc gaagccgcgc ggccggttgg
    26641 tcttcacgac gcagcggttg gtgttcgagc tgccgaaagc cgtcgcggtg accgccggcc
    26701 aggcgctcat gaacgagatg gtcaatcacc tgccgatcga cgttccgcgc gatcgtctcg
    26761 acgccgtcga gctcggcaag tatcgcaaga gcgacaacgt gatcgtcgtc cgcaccgagg
    26821 acgccgagta caagttcttg ttccgcacgc cggtcaagga gtgggaagcc acgctgaagc
    26881 aggcgctgca cgacgacaag gtcgcgctcc agcatctgct cgatcgcttg aagcccaaag
    26941 agaacccacc gccgtatcgc tgagcacctg cgggttgatg cgtcgcgggc ccgcgggtat
    27001 cactaggagg ctgcgatgtt gttgcgaacc tcgatcgcca tgctcatgct cgtgtgcgcg
    27061 acggcccatg ccgaggtgaa ggtcgactgg gcgcgcggcc tcgtcaccgc cgatgcggtc
    27121 ggagttgccg atcggcatgc gccgaatcca gcggtcgcgc ggggcacgtc gcggcgcggc
    27181 gccgaggacg cggcgaagaa gctgctcgcg gcgaaggtcg gcgagctccc ggtcgcgggt
    27241 ggcagcaccg tcgcagcgaa ggcgaaggac aaggcggtga aggagcggat cgagcgcgcg
    27301 gtcgctaatg cgatcacgct cgccgcggat cccgagaccg acggcgcctg gaaagtgacg
    27361 ctcgcggtgc cgatcgaggc ggtgcgccag gcgattgccg gaccgcgtgc gttgccggcc
    27421 gatggcgaca agggcccggt cgtggttgtc gtcgacagcg cgaccgcaaa accggcggtt
    27481 gggtggacga tcgccggtgt cgaggcgccc gttctgtggg tgaccgatgt tccggcatgg
    27541 gccaaggacg cgccgcacgc gaccgcgaag ggtgcgaaag gcggcgcgat cgaggtgacg
    27601 ggaatcgatg ccagcgctgc gacgttattc gtggtcgtca cgaagtgatc ggcgtggccg
    27661 cacggcgcga gttgcgatgg cgctctactg gggcggggac tggcttctgc taggctacgt
    27721 ggcgtgatcc gctgtgtcct acctctcgcc atggtggccg cgcttgtctc cgtcgcgcat
    27781 gccgaggacg tcgtcgctta tcaagcggaa ggcgatgcgc cgaccgcctc tgccgacgcg
    27841 cgcgtgatgg cgctcgacga agcgttcgca catgccgtcg gcaatgcgct cgccgatctg
    27901 gtggccggtg acgtgcgtac cgcgcgcaaa ggcgagctcg atcgcgagat cgtcgggcgc
    27961 gctcggctct gggtcgcgaa gttcgcggtg acgaaggacg agaccgtcga caatcggcgc
    28021 gagctcacgg tcagcgtgaa gatcgatcgt gacaagatgc gtgcgcggct tgccgagctg
    28081 aagatcgcga cgaaagatgc tggcaccgcc accgagccgg cacccgcgca gcgcacggcg
    28141 acgatcctga tgcgcctggc actgccggcc ggcgtgcgcg ccgtgtatgg accgaatgca
    28201 gtgcaggatg cgcctggcct gatcgcgctg acgaacctgt tgcgaaacgc cggcttcgcg
    28261 gtgcgccgcg cgcctgcctc gggaccggca ccgcgtgccg acggcgacct gccgctctcc
    28321 gacgaggagg ccgacgcgct cgcgaacgag gcaaaggccg atgtgatggc gatcgccggc
    28381 gtcagcctcg ggccgccagt tgtggtgcgc gggcaaccga cgaacgcagc gcttgtcact
    28441 gctcacgtgc gcatcgtcga tcgcaaggac aagaaggtgc tcgggcaggg cgctgcggct
    28501 gcagctgcac gtggcgaaga tccgggctac gcgg
//
LOCUS       DASUFX010000348         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1028212, whole genome shotgun sequence.
ACCESSION   DASUFX010000348 DASUFX010000000
VERSION     DASUFX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028212"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2422)
                     /locus_tag="VFV99_13970"
     CDS             complement(<1..2422)
                     /locus_tag="VFV99_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060469.1"
                     /translation="MSTLDQSQARKGLSDYFSASHARVERWRTVHRLARSLAAGGKPD
                     ALRTELVEALQDLAPIEDLCGYPGPGLMAALKERLQTSDWTGLARLAQRISVSLLSNS
                     YRDDPGAWLVDEEGEAHVPEVLPPGLGRGQARKPYFEVLIVTPGERSTWGDMREAFRG
                     LRRDTDAFVYEPVVVGSFEDAILAAIINYNVQAVVVFDGFAYPSQYTVHDLRDLLAPH
                     VPQGGNVQGADLGTALSRVLLAYRPELDIYLATDRDVGALAGADEAAGIRRIFYGLEE
                     MLEIHLSVLDGIADRYATPYFDNLKKYAQRPIGTFHALPVARGKSIFKSNWIRDMGEF
                     YGTNLFLAESSATTGGLDSLLEPTGNIKVAQEKFARAFGADRAFFVTNGTSTSNKIVH
                     MALLAPGDIVLIDRDCHKSHHYGLVLAGAQPYYIDAFPLTQYSMYGSLAIRPIKQALL
                     DLKAEGKLDKAKMVVLTNCTFDGHVANVEQTMLECLAIKPDLIFLWDEAWFGFARFSP
                     FLRRRTAMGAAAKLRAMMRDPAYRARYATFKAKVGEIDPKNKKLLETTLLPDPDKVRI
                     RVYETDSVHKSMSSLRQGSVVIVADQDFHTIEPMFKESFFTHTSTSPNLQIIATLDLA
                     RRQVELEGYELVQRAIQLAIELRRDINGHPLISKYFRAATPAEMIPEQYRQSGFADYG
                     APGWNVAKTMQALDSDEFFLDPTRVTLLCGNAGYDGTQFKALLASEYDIQINKTSRNS
                     VLVQININNTLSDVGHLVKALADIARAVEKRLAEGGAAEQAVFAARVKSLVSDVPDLP
                     DFSRFHDAF"
BASE COUNT          462 a          922 c          987 g          506 t
ORIGIN      
        1 ggaatgcgtc gtgaaagcgc gagaagtccg gcaggtcggg cacgtcggac acgagcgact
       61 tcacgcgcgc cgcgaagacc gcctgctcgg ccgcgccccc ttcggcgagg cgcttctcga
      121 cggcgcgcgc gatgtcggcg agcgccttga cgaggtgccc gacgtcgctc agcgtattgt
      181 tgatgttgat ctggacgagc acgctgttcc gcgacgtctt gttgatctga atgtcgtact
      241 cgctcgccag caacgccttg aactgcgtgc cgtcgtaccc ggcgttgccg cacaggagcg
      301 tgacgcgcgt cgggtcgagg aagaattcgt cggagtcgag cgcctgcatc gtcttcgcga
      361 cgttccagcc cggcgcgccg tagtcggcga agccggactg gcggtactgc tcgggaatca
      421 tctcggccgg cgtcgccgcc cggaagtact tggaaatgag cgggtggccg ttgatgtcgc
      481 gacgcagctc gatcgcgagc tggatcgccc gctgcacgag ctcgtagccc tcgagctcca
      541 cctgcctccg cgcgaggtcg agcgtcgcga tgatctgcag gttgggtgac gtcgacgtgt
      601 gcgtgaagaa cgactccttg aacatcggct cgatcgtgtg gaagtcctgg tcggcaacga
      661 tcacgaccga tccctggcgc agcgacgaca tcgatttgtg caccgaatcg gtctcgtaga
      721 cgcggatgcg caccttgtcg ggatcgggca gcagcgtcgt ttccagcagt ttcttgttct
      781 tgggatcgat ctcgccgacc ttcgccttga aggtcgcgta gcgcgcgcgg taggcgggat
      841 cgcgcatcat cgcgcgaagc ttcgccgcgg cgcccatggc ggtgcgtcgc cgcaggaacg
      901 gcgagaaacg cgcgaagccg aaccacgcct cgtcccacag gaagatgagg tcgggcttga
      961 tggcgagaca ctcgagcatc gtctgctcga cgttcgcgac gtggccgtcg aaggtgcagt
     1021 tggtcagcac caccatcttg gccttgtcga gcttgccctc cgccttcagg tcgagcagcg
     1081 cctgcttgat cggccggatc gcgaggctgc cgtacatcga gtattgcgtc agcgggaagg
     1141 cgtcgatgta gtacggctgg gcaccagcga gcacgaggcc gtagtgatgc gacttgtggc
     1201 agtcgcggtc gatcaggacg atgtcgccgg gcgcgagcag cgccatgtgc acgatcttgt
     1261 tcgacgtcga cgtgccgttg gtgacgaaga atgcgcggtc ggcgccgaat gcgcgcgcga
     1321 acttctcctg cgcgaccttg atgttcccgg tcggctcgag caggctgtcg aggccgcccg
     1381 tcgtcgccga cgattcggcg aggaacaggt tcgtcccgta gaactcgccc atgtcgcgga
     1441 tccagttcga cttgaagatc gacttgccgc gcgcgaccgg cagcgcgtga aacgttccga
     1501 tcggccgctg cgcatacttc ttgagattgt cgaagtacgg ggtggcgtat cggtcggcga
     1561 tgccgtcgag gacggaaaga tggatctcga gcatctcttc caatccgtag aagatgcggc
     1621 ggatgcccgc ggcttcgtcg gcgcccgcga gcgcgccgac gtcgcgatcg gtcgcaagat
     1681 atatgtcgag ctcgggacga taggcgagca gcacgcgcga cagcgcggta ccgaggtcgg
     1741 cgccctgcac gttgccgcct tgcggcacgt gcggagcgag cagatcgcgc aggtcgtgca
     1801 ccgtgtattg cgacggatag gcgaacccgt cgaacacgac caccgcctgg acgttgtagt
     1861 tgatgatcgc cgcgaggatg gcgtcctcga agctgccgac gacgaccggc tcgtagacga
     1921 acgcgtcggt gtcgcggcgc agtccgcgga acgcttcgcg catatcgccc cacgtggagc
     1981 gctcgcccgg cgtgacgatg agcacttcga aatagggctt gcgggcctgc ccgcggccga
     2041 gcccaggcgg cagcacctcg ggaacgtgcg cctcgccttc ttcgtcgacg agccatgccc
     2101 cgggatcatc ccgatagctg ttcgaaagga gcgaaacgct gatccgttgc gcgagccgcg
     2161 cgagaccggt ccagtcggac gtctgcagcc gctccttcag cgccgccata aggcccggcc
     2221 cgggatatcc gcagaggtcc tcgatcggcg ccagatcctg cagcgcttcg acgagctcgg
     2281 tgcgaagcgc atcgggcttg ccgcccgcgg ccagcgaccg ggcgagccgg tgcaccgtgc
     2341 gccagcgctc gacgcgcgca tgcgacgcgg aaaagtagtc cgagagtccc ttgcgtgcct
     2401 gcgattgatc gagtgtcgac atccttctct ctcctttcga tgcgaaagcc gcggaagctt
     2461 cgcggccgtc cacgacgctt tcgtgcgcga aatgcgctgc gagaggcgcc gtccggcgca
     2521 gtgctcgatg aacgcgttgc cgcgctcgcg aaccgtgtcg ccaacgggag gcgctgtgat
     2581 gcgcggccca cggccgcgcc tccggcgcat ccgactcgtt cacatcgtct gcttgcgaag
     2641 gagtcgagtc ttcgcatttc tcgacacgga tgacggctcg tgtgccgtca gcggagcgta
     2701 cgcacgcagc cgcggctgct gcgtcttcgc gagagcgcgg catcgcgccg ccgcgctgaa
     2761 gggacgcttc gtcgttgggg cggtagccgc gatgcgtgcg cagcgtgcgc cgacgaatcg
     2821 tcgaggccac gcctccagtc ggaccgcgtc ttcagtcaga ccgcgcctgg cggcgcc
//
LOCUS       DASUFX010000349         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1166604, whole genome shotgun sequence.
ACCESSION   DASUFX010000349 DASUFX010000000
VERSION     DASUFX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166604"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFV99_13975"
     CDS             <1..324
                     /locus_tag="VFV99_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060470.1"
                     /translation="QWGGPFVEINRWMRGLLGDDVKNWKQYDPTTLIDKLKPGTLALY
                     MDCGTEDGFALNNGMAWLHDLLLEKKIEHAYFVGPGGHDFGFWKPRLPESLKFLRDHV
                     AKPAT"
     gene            complement(325..1755)
                     /locus_tag="VFV99_13980"
     CDS             complement(325..1755)
                     /locus_tag="VFV99_13980"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060471.1"
                     /translation="MGHIAKTDAELVEASRRGEREAFGLLVSRYQDVVCAVSFSSTGN
                     WALSEDVAQDTFIAAWRQLGQLRETGRLRSWLCGIARNLARKARKRTDREAAVDDDVP
                     ALDGNPFDATAQAEVDRVVREALARIPDSYRETLVLYYCENRSIRDVAAVLGVSEDAA
                     MQRLTRGRRYLATGVSELVERSLRGTRPRKGFAAAVIAALPLVGVSRVDASPNPEGST
                     MLKIGLAAAVFVAIGTTAIVAKRSDGPSAKPQVGTLAATPVVAQKAVSGAARRSGGHV
                     NAPVLPGGGAVTAHAIDDDDAPRLIGQDTIAKLKLDQGPSRGPANAPVTIVLFQDLMC
                     KYCGIVLGTIDQIWDEYPGKLRLVVKQLPVHSEARLAAEASLAADAQGKFWDLHDVMI
                     ANQADLSHDAIIGYAQQAGLDVTRLTAALDQHTYQNALETEMDAAKDIGVQATPTFLV
                     NGQEVRGALPPEAFRTVIDEALAASK"
     gene            complement(1840..>2475)
                     /locus_tag="VFV99_13985"
     CDS             complement(1840..>2475)
                     /locus_tag="VFV99_13985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5060472.1"
                     /translation="GTLFLDEIGELPLPAQVKLLRVIQTGTFCRVGGSDEIATDVRLI
                     AATLRSLPALVADGAFREDLFYRVNVFSLELPPLRERRDDIAPLASALLQQLCARLGV
                     PVPTVPSAVLAQLEAHDWPGNVRELANVLEAALIISDGSELVLPRALTVRRAPATSFD
                     GAATHAIEAALRATRGKIYGAGGAAARLGLKPATLQSKMRKLGIERRAFTA"
BASE COUNT          415 a          881 c          763 g          416 t
ORIGIN      
        1 cagtggggcg gcccgttcgt cgagatcaat cgctggatgc gcggcctgct cggcgacgac
       61 gtcaagaact ggaaacagta cgacccgacg acgctcatcg acaagctgaa gcccggcacg
      121 ctcgccctct acatggactg cggcaccgaa gacggcttcg cgctcaacaa cggcatggcg
      181 tggctgcacg atctcttgct cgaaaagaag atcgagcacg cgtacttcgt cggtccgggc
      241 ggccacgact tcggcttctg gaagccgcgg ctacccgaga gcttgaagtt cctgcgcgat
      301 cacgtcgcca agccggcgac ctgatcactt cgaagcggcg agcgcttcgt cgatcaccgt
      361 gcggaacgcc tcgggcggca gcgcaccgcg cacttcctgg ccattcacga gaaacgtcgg
      421 cgtcgcctga acgccgatat ccttggcagc atccatctcg gtctcgagcg cgttctgata
      481 cgtgtgctgg tcgagcgccg cggtcagcct cgtcacatcg agtcccgctt gctgcgcata
      541 cccgatgatc gcgtcgtgcg acagatcggc ctggttcgcg atcatcacgt catgcagatc
      601 ccagaatttg ccctgcgcat ccgctgcgag cgatgcctcg gcggcgagcc gcgcctcgct
      661 gtgcaccggc agctgcttga cgacgagccg cagcttgccc gggtactcgt cccagatctg
      721 atcgatcgtc cccagcacga tgccgcagta cttgcacatc agatcctgaa acaacacgat
      781 cgtcaccggc gcattcgccg ggccgcgcga cggtccttgg tcgagcttca gcttcgcgat
      841 cgtgtcctga ccgatcagcc tcggcgcgtc atcgtcgtcg atcgcatgcg ccgtgaccgc
      901 tcctccacca ggcagcactg gtgcattgac gtgcccaccg cttcggcgtg ccgctcccga
      961 caccgctttc tgcgcgacga ccggtgtcgc cgccagtgtt cccacttgcg gtttcgcgct
     1021 cgggccatcc gaacgcttcg cgacgatcgc cgtcgtacca attgccacga acactgctgc
     1081 tgcgagtcca atcttcaaca tggtcgatcc ttccgggttt ggagaagcat cgacacgaga
     1141 cacacccacg agcggcagcg ccgcgatgac ggctgccgca aagcctttgc gcggccgcgt
     1201 accgcgcagc gaccgctcga caagctcact cacaccagtc gccaggtacc ggcgcccgcg
     1261 cgtcagtcgc tgcatggccg catcctccga gacgccgagc acggccgcga cgtcgcgaat
     1321 cgatcggttc tcgcagtagt acagcaccag cgtctcgcga tagctgtcgg gaatgcgcgc
     1381 cagcgcctcc cgcaccacgc gatcgacttc ggcctgcgcg gtcgcatcga acggattacc
     1441 gtcgagtgcc ggcacgtcgt cgtcgacagc cgcctcacga tccgtgcgct tgcgtgcctt
     1501 gcgcgcgagg ttgcgcgcga tcccgcacag ccacgaacgc agccgcccgg tttcgcggag
     1561 ctgaccgagc tggcgccacg cggcgatgaa cgtgtcctgc gcgacgtcct cgctgagtgc
     1621 ccaatttccg gtcgagctga acgagaccgc acagaccacg tcctggtacc gactcacgag
     1681 gagcccgaac gcttcgcgct cgccgcgcct gctggcttcg acgagctccg catccgtctt
     1741 cgcgatgtgt cccatgtcgc ccctgtgttc ctcgccggcg ccgaaacctg acaagaaaag
     1801 cgttcgtggc tgctaaccag ccacaaaccc agggcattcc tacgcagtga acgcgcgccg
     1861 ctcgatcccg agcttgcgca ttttgctctg caaggtggcg ggcttgagcc cgaggcgcgc
     1921 ggcagcgcca ccggcgccgt agatctttcc gcgcgtcgca cggagagcgg cttcgatcgc
     1981 atgcgtggcg gcaccatcga agctcgtcgc gggcgctcgc cgcaccgtca gcgcgcgcgg
     2041 cagcacgagc tcgcttccgt cgctgatgat caacgcggcc tcgagcacgt tcgccagctc
     2101 gcgcacgttg cccggccagt cgtgcgcctc gagctgcgcg agtaccgccg acggcacggt
     2161 cggtacgggc actccgagcc gtgcgcacag ctgctggagc agcgcgctcg cgagcggagc
     2221 gatgtcgtcg cggcgttcgc gcagcggcgg cagctcgagc gagaacacgt tgacgcgata
     2281 gaacagatcc tcgcggaacg cgccgtcggc gacgagcgct ggcagtgaac gcagcgtagc
     2341 cgcgatcagg cgcacatcgg tcgcgatctc gtcgctgccg ccgacgcgac aaaacgttcc
     2401 ggtctggatc acgcggagta gcttcacttg cgcgggcagc ggcagctctc cgatctcgtc
     2461 gaggaacagc gtgcc
//
LOCUS       DASUFX010000350         9954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_194477,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000350 DASUFX010000000
VERSION     DASUFX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194477"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFV99_13990"
     CDS             <1..385
                     /locus_tag="VFV99_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060473.1"
                     /translation="FAGDWHGNRVGDTPVVRVGPGIDATLTIDTIDKHGLRGHLLVNT
                     SVPGVPLVDAPVMSLEAAEADALATMTFSGAPLRVYLAFVPVLGVGGDALAVIALYDS
                     HRVELRLLRSDPDPLYAIFALGEGS"
     gene            404..2335
                     /locus_tag="VFV99_13995"
     CDS             404..2335
                     /locus_tag="VFV99_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060474.1"
                     /translation="MTCPHCHAPLTADARFCPACGKATSPTESGTSWPAAGSAPDLVG
                     REIAGRYRIVKKLGEGGMGAVYRGEQLSLKRAVAVKVLKPELGANQMILRRFNAEAEA
                     VAKLSHPNTVNIYDFGQDSDGSLFIAMEFIEGRSLREAIQKEGPFSPTRSIAIALQVA
                     ASLSDAHAHSIVHRDLKPDNVMLQDRGRNKDVVRVLDFGIAKLRDDNRATQQAMTQAG
                     DMLGTPQYMAPEQIRGEAIDGRTDIYALGCMIYEMVTARLPYEAPTIMAMLSKHLLET
                     PVPPSQRRPDLAIPASIDQLVLGAMAKDPNARAATMEQFAEHLQSLLATLPADPNRSA
                     GMSVQQGAATPFAPPTQPPPAYTPAGFAPPTTPPPSGGASYAAPTPPPAGVAYAPPSA
                     SAPTAASGKSRTPMWIALGVLALGGAGAGIFFATRDSGTTAKADSEEPDEHENTPDKD
                     EPDEHEQPDEPAQPDKWETPANNGEDPWAPKAQGGRAPKNKVTAAKPAAANPRAIGTD
                     ATPIPDGAKVRVGSGYTSQVSSAAHVHVNAAKGILIAMAPLLPGTNDPQKLGEMWTSQ
                     TGVTATGSMKIMSAGKLRPALGFAGEVNGVPVNQVVVLYIEPDYRVGVLYQAPTASFS
                     DSAFQRDVMTWFARNVVLP"
     gene            2425..3450
                     /gene="selD"
                     /locus_tag="VFV99_14000"
                     /pseudo
     CDS             2425..3450
                     /gene="selD"
                     /locus_tag="VFV99_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            3471..3803
                     /locus_tag="VFV99_14005"
     CDS             3471..3803
                     /locus_tag="VFV99_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060475.1"
                     /translation="MRLWLVVAVVLAACGGEGEFHDAASPPLCALCTSDTDCETGICW
                     TYGNGQSKCTHTCTAGEEAADCPTGRCNGMGYCMCPGYEPPTDAGIDDAQKDAPEVPP
                     ADAPTDGA"
     gene            complement(3809..4303)
                     /locus_tag="VFV99_14010"
     CDS             complement(3809..4303)
                     /locus_tag="VFV99_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060476.1"
                     /translation="MRSAHVIPLLLLCACPAACKSERAAETRKEPSAMAPQLSGRIPR
                     QMRNCPSGVPSAKTVATPTADGVNLTITSDNPAARRRIVALAQMHATWGEPIWAMPYH
                     TGMHGGPGTIGLCPIIHAKTIVTQREIPEGVLIHVRSEIPGMESRLQELTKLRVNALE
                     PPSS"
     gene            complement(4334..4717)
                     /locus_tag="VFV99_14015"
     CDS             complement(4334..4717)
                     /locus_tag="VFV99_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5060477.1"
                     /translation="MIDHIGIAVTDLDKSVAFYTKALAPLGYELLRKFDIPSGGKVAG
                     FGVAGKPDFWIAQGEVKQHIHVAFRATGRANVREFYDAAMAAGGKDNGAPGVRPHYHE
                     HYYGGFVYDPDGHNVEACCHEPFLG"
     gene            4755..5492
                     /locus_tag="VFV99_14020"
     CDS             4755..5492
                     /locus_tag="VFV99_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060478.1"
                     /translation="MRGGLVLLTAVLVAAPGSAHAGRTFYGWLYGTEVMPERGAELQS
                     WITEENFDNLKQDSWQFSAQIGITDQLELGLPLEIDWFRDTTTMPPTAGTVFSRFGAD
                     LRYRFVTQDPVDAPAIVPLLRVGVKRLITQRDGFQPEADLVVSYESGIVQAEVDLGFL
                     AQVGIQGDDYYAFRPGAGVSVLAIDDLRFGAEFFAEINNADDSWYIAGPNMAWTHGRF
                     WMSAAFGVGLDQDRIKTAPRVQWGIAF"
     gene            5501..6139
                     /locus_tag="VFV99_14025"
     CDS             5501..6139
                     /locus_tag="VFV99_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5060479.1"
                     /translation="MAVVAAAGSAHAGGSVTGKVTVTDANGKPVTADVVVYVTGFPEK
                     GDTSAPAATIAQKGRKFVPDLVAITVGESVTFPNNDPFLHNVFSQSPPRKFDLGSFKK
                     GESKEKQFPNAGVIDVYCNIHPEMAATILVLPNKRHTRTAPDGSFKIDGVPAGRWTVF
                     AYARRATKPVSAAVTVTDGQDATVNLSLVRGAEPPHVNKYGEKYKEPTTPYK"
     gene            6155..6871
                     /locus_tag="VFV99_14030"
     CDS             6155..6871
                     /locus_tag="VFV99_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060480.1"
                     /translation="MRALLIVALVGCASGPNVDAVIASHHHDRTIAAPAVRAPRPWEV
                     GQWTLSKTSSQGHVGTIRRSIVAKDACGFWLEVVATSPRGRTITKTCYRSQLASSANP
                     AAELDALQAIMIRQGGRTIVIDFRNGKNPAKKLQMQTQLQQRVSLAWQSPSSDELATI
                     VVPAGRFEGAAHVVARLWVEQSMQTAQAWLHPAVPIDGVVKESATDGSELELLDYGLT
                     GAKSELPSFDEQLSESGLAD"
     gene            complement(6868..7746)
                     /locus_tag="VFV99_14035"
     CDS             complement(6868..7746)
                     /locus_tag="VFV99_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060481.1"
                     /translation="MTADRVQQLSAASGLAASSPTAALSAWESLLATARASWPRVKLD
                     EAKLVTFIGERLAGSDLVAAIAAAPAADLAIAAACAAQEPTAHAAFDAVLTEVDAAGA
                     STGAERDQIEEVKQLLRLQLLVTKDGKPPGIAGYRGRGPLRGWVRITATRELIRHKKK
                     RTREVSFSQSLEKYLTSGVDPALEALKAEYRTEFALALNEAIEDLTPEDRTLLRQAIV
                     DDMSIDAIGAAFGVHRATAARWLTRARAAMVSATHNRLAARLKLPVEQIESVIRLVQS
                     KLDASVIRYLRDRKSD"
     gene            7783..>9954
                     /locus_tag="VFV99_14040"
     CDS             7783..>9954
                     /locus_tag="VFV99_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060482.1"
                     /translation="MECLDANVVQDLMSGALDTAQREGVLGHLDTCEDCRELLGITAR
                     DTLRDQLKQNAYPEEVAADARRVVNAAMTLPSANGANVGMLETAAGTVAPGHDVGLAA
                     TLAPDLLATPVMGTEVAGEPPTQGRKLGRYTLIERLGAGAMGVVWRAEDPTLSRQVAV
                     KLLRRHDESLTERLVREAQSMAQVNHPNVVTVYEVGQGVDGTTFIAMELVPGESLRAW
                     QTKARHTIPALVQAYIAAARGLAAAHSAGIIHRDFKPDNVLVGNDGRVRVTDFGLAAA
                     KQTVSASGRLQQISDVNLTTSGSVMGTPAYMAPEQFVGGNVDPRTDQFNFCVALYEAL
                     YGERPFDGKNFHELGDNVCEGKLKPEPANTEVSGALRAIVLRGLSVKPGDRFATMDEL
                     ILELGRDRAKPWRRTSIASAAVAVALGLGLVSDWIVRDRVTSEIRHSFALTREQQKRV
                     VNRLIESFKVTSEVAYREQALREVAGHHDQADFGLGDPETDQADLEKLHNTLSSTDWV
                     KNGTATLAIADYKGRLLFTSAGDTWGGDLKALVPVKRAIDAGKGDSVTVLPYADPGLA
                     TTGILGAHPPTRGLAVLFERTLALGDKASEGSEARGFYLQLQDGKQLLDEISLDKQTL
                     LGLVALDGTAIGELPPALIKAAPSNEESAEIRVDGRVYQVRSLPMTGLAAQGTIAHVV
                     MARPLDGVLSLFPGARLVFALTAFGALLLAATTYVSARRITL"
BASE COUNT         1699 a         3279 c         3375 g         1601 t
ORIGIN      
        1 ctttgccggc gattggcatg gcaatcgggt cggcgatacc cctgtggtcc gcgtcggtcc
       61 aggcatcgac gcgacactga caatcgacac gatcgacaag cacggcctgc gtggtcacct
      121 gctcgtgaac acgtcggttc caggcgttcc tctggtcgac gcgcctgtca tgtcgctcga
      181 agcggccgag gccgacgcgc tcgcgacgat gacgttctcg ggcgcaccct tgcgcgtgta
      241 cctcgcgttc gttccggtgc tcggcgtcgg cggcgatgcg ctcgccgtga tcgcgctcta
      301 cgactcccac cgcgtcgagc tgcgcctatt acgtagcgac cccgatccgc tctacgccat
      361 cttcgcgctc ggcgagggct cttgagctat tctcgacgtc tccgtgacgt gcccgcactg
      421 ccacgcgccg ctcactgctg acgctaggtt ttgtcccgcc tgcggcaagg caacgtcacc
      481 gaccgagtcc ggcacgagct ggccggccgc gggcagcgca cccgacctcg tcggccgcga
      541 gatcgcgggg cgctaccgca tcgtcaagaa gctcggcgaa ggcggcatgg gcgccgtgta
      601 tcgcggcgag cagctttcgc tcaagcgcgc ggtcgcggtg aaagtgctca agcccgagct
      661 cggtgcgaat cagatgatct tgcgccggtt caacgcggag gccgaagcag tcgcgaagct
      721 cagccacccg aacacggtca acatctacga cttcggccag gacagcgacg gctcgctgtt
      781 catcgcgatg gagttcatcg agggtaggtc gctgcgcgag gcgatccaga aggaaggccc
      841 gttctcaccg acgcgctcga ttgcgatcgc gctccaggtc gcagcgtcgc tctccgacgc
      901 gcacgcgcac tcgatcgtcc accgcgatct caagcccgat aacgtgatgc tccaggatcg
      961 cggccgcaac aaggatgtcg tacgcgtgct cgatttcggc atcgcgaagc tgcgcgacga
     1021 caatcgcgcg acccagcagg cgatgacgca ggcgggcgac atgctcggca cgccgcagta
     1081 catggcgccg gagcagatcc gtggcgaagc gatcgacggc cgcaccgata tctacgcgct
     1141 cggctgcatg atctacgaga tggtcacggc gcgcctgccg tacgaggcgc cgacgatcat
     1201 ggcgatgctg tcgaagcatc tactcgagac tccggtgccg ccatcgcagc gccgacccga
     1261 tctcgcgatt cccgcgtcga tcgatcagct cgtgctcggc gcgatggcaa aggatcccaa
     1321 cgcacgcgct gccacgatgg agcagttcgc cgagcatctg cagtcgctgc tcgcgacgct
     1381 gccggcggat ccgaatcgca gcgctggcat gtcagtgcag caaggcgcag caacgccgtt
     1441 cgcgccaccg acgcagccgc cacccgcgta cacaccggct ggcttcgcac cgccgaccac
     1501 gccgccaccg tcgggaggcg cgagctacgc agcgccgacc ccaccacccg ccggtgtggc
     1561 ctacgcgccg ccgagcgcat ctgcgccgac ggccgcgtcg ggcaagagcc gcacgccgat
     1621 gtggatcgcg ctcggcgtgc tcgcactcgg tggcgccggc gccggcatct tcttcgcgac
     1681 gcgcgactcg ggcacgactg cgaaagcgga tagcgaggag cccgacgagc acgagaacac
     1741 gcccgacaag gatgagccgg acgagcacga gcagcccgat gaacccgcgc agcccgacaa
     1801 gtgggaaacg ccagcgaaca acggcgagga tccgtgggca ccaaaggcgc agggcgggcg
     1861 cgcgccgaag aacaaagtca ccgccgccaa gccggccgct gcgaatccac gggcgatcgg
     1921 caccgacgcg acgccaattc ccgacggtgc gaaggtgcgc gtcggttcgg gatacacatc
     1981 gcaggtctcg agcgcggcgc acgtgcacgt caacgccgcg aaggggatcc tgatcgcgat
     2041 ggcgccgctg ctaccgggca cgaacgatcc gcagaagctc ggcgagatgt ggacgtcgca
     2101 gaccggcgtc accgcgacgg gcagcatgaa gatcatgagc gcgggcaagc tacggccggc
     2161 gctcggcttc gccggagagg tcaacggcgt gccggtcaat caggtagtcg ttctctacat
     2221 cgagccggac tatcgcgtcg gggtgctgta tcaagcgccg accgcatcgt tcagcgattc
     2281 ggcatttcag cgcgacgtga tgacgtggtt cgcacgtaac gtcgtcctgc cttagctgct
     2341 acgctgcgcg ggatgactcc cgaagcgaaa gcagccattc gcctgacgca atacgcgcgc
     2401 ggcggtggtt gagccgccaa gctccggctc ggcgagctgg cacatgttct cgatcacctc
     2461 gaaagacgag gatcgggagg ggtgcgtccg gacgtcatcg tcgatcacga gtactcggac
     2521 gatgcagcca tcgtgcgcat gggcggagcg cgcgatgtcg tgctcacggc cgacgtgatc
     2581 gcaccactcg tcgacgatcc agatgcattc ggtgcgatcg cggcggcaaa ctcgctctcc
     2641 gacgtctggg cgatgggcgg cgaaccgcgc ttcgcgctca acctcgtctt ctttcccgac
     2701 gaccagctcg acctcagtgt gctcgacgcg attctcgccg gtggcgcgcg cgtgtgcgcc
     2761 gaagcaggtg cagccgtcgt cggtggtcac acggtgcgcg accaggaagt gaagttcggc
     2821 ttgtcggtca ccggtgatgt cgcagcgggc gaggagtggt cgaatcgcaa ggcgcagccc
     2881 ggtcaggcgc tcgtgctcac gaaggcgctc ggcaccggcg tgatcggcca ggcgatcaag
     2941 aaagggctcg cgagcgacgt cgcgatgact gcggcgatcc gttcgatgac gacgctcaat
     3001 caaggcgccg cgaagatcgg ccgtgcgttc ggcacgactg cggcgaccga catcacgggc
     3061 ttcggtctgc tcggccacct gcgtaacatc gtgcgaggta gcaacgtcgc agcgacgatc
     3121 gagctcgccg cattgccgct actgcccgac gcgatcgagc acatgcgcgc gaaggtctgc
     3181 cccgcgggca gcaaagcgaa tcgcgcgcac gttgccgccg acactgcgtg ggccgacggc
     3241 acgccgctcg tcgcggaggg cgatggtagc gagcgcgatc tgctcgcctc gctcgcatgt
     3301 gacgcgcaga cgagcggcgg cctcttgctg tgcttgccgc cgggacgcgc ggacgcgtgc
     3361 gtcaccgagc tgcgcgctct cgggttgcca gcggctcgca ttggcgcgct cagtgcgcct
     3421 ggtgagggcg cgcacatcgt gctcgcgtga tcatcgagta cgctgcgcgc atgaggttgt
     3481 ggttggtcgt tgccgtcgtc ctcgccgcgt gcggtggcga aggcgaattc cacgacgctg
     3541 cgagcccgcc gctctgcgcg ctgtgcacga gcgacaccga ttgcgaaacc ggcatctgct
     3601 ggacgtacgg caacggccag agcaagtgca cgcacacgtg cacggccggg gaagaggcgg
     3661 ctgactgtcc gacaggccgc tgcaacggta tgggctactg catgtgcccg ggctacgagc
     3721 cgccgacgga tgccggcatc gacgacgcgc agaaggacgc gccggaagtg ccgcccgctg
     3781 atgctccgac tgacggcgcg tagatcaact agctcgacgg cggctcgagc gcgttcacgc
     3841 gtagcttggt cagctcctgg agccgactct ccatgcccgg gatctcggag cgcacgtgga
     3901 tcaggacgcc ctcgggaatc tcccgctggg tcacgatcgt ctttgcatgg atgatcggac
     3961 aaaggccgat cgtgcccgga ccgccgtgca ttccggtgtg gtacggcatt gcccagatcg
     4021 gctcgcccca tgtcgcgtgc atctgagcga gtgcgacgat cctgcgccgc gcggccgggt
     4081 tgtccgaggt gatcgtgagg ttgacaccgt cggcggtcgg tgtcgcgacc gtctttgccg
     4141 agggaactcc gctcggacaa ttgcgcatct gccgtggaat tctgccgctg agctgcgggg
     4201 ccatcgccga cggctctttg cgcgtctcgg cggcacgttc gctcttgcac gccgccgggc
     4261 aggcgcacaa cagcagcagc ggaatcacgt gtgcggatct catgcacacg caacgctgcg
     4321 acgcgcgtgc cagctagccg aggaacggtt cgtgacagca ggcttcgacg ttgtgcccgt
     4381 cggggtcgta cacgaagccg ccgtagtagt gctcgtgata gtgcgggcgc acgccgggtg
     4441 cgccgttgtc cttgccgcct gcggccatcg cggcgtcgta gaactcgcgg acgttcgcgc
     4501 ggccggttgc gcggaacgcg acatggatat gttgtttgac ctcgccctgc gcgatccaga
     4561 agtcgggctt gccggcgacg ccgaacccag cgaccttgcc gccgctcggg atgtcgaact
     4621 tgcgcagcag ctcgtaaccg agcggcgcaa gcgccttggt gtagaacgcg acgcttttgt
     4681 cgagatcggt gaccgcaatg ccgatgtgat cgatcatgcg cgcaccatat caaacgatgg
     4741 agtaaggtcg cctcgtgcgt ggtggactcg ttttgttgac ggcggtgctc gtggccgctc
     4801 ccggttcggc gcatgcaggc cgcacgtttt atggctggct ctacggcacc gaggtgatgc
     4861 ccgagcgcgg cgccgagctg cagtcatgga tcaccgagga gaacttcgac aacctcaagc
     4921 aagacagctg gcagttcagc gcgcagatcg ggatcacgga ccagctcgag ctcggcctgc
     4981 cgctcgagat cgattggttc cgcgatacga cgacgatgcc gccgaccgcg ggcaccgtgt
     5041 tctcgcgctt cggcgccgac ctgcgttacc gcttcgtcac ccaggatccg gtcgatgcgc
     5101 cggcgatcgt gccgctcttg cgtgttggcg tgaagcggct gatcacgcag cgcgacggtt
     5161 ttcaaccgga ggccgacctc gtggtgtcgt acgagtcggg catcgtgcag gcggaagtcg
     5221 atctcggctt tctcgcgcag gtcggcatcc agggcgacga ctactacgcg tttcgccccg
     5281 gcgctggcgt cagtgtgctc gccatcgacg atctgcgctt cggcgccgaa ttcttcgccg
     5341 agatcaacaa cgcggacgac agctggtaca tcgcgggacc gaacatggcc tggacgcacg
     5401 gccggttctg gatgtccgcc gcattcggcg tcggtctcga tcaagaccgg atcaagaccg
     5461 cgcccagggt ccaatggggc atcgcgttct aatcccgatt gtggccgtgg ttgccgcggc
     5521 gggcagtgcg cacgcaggcg gcagtgtcac cggcaaggtg acggtgaccg atgcgaacgg
     5581 caagccagtg accgccgacg tcgtcgtcta cgtcaccggc tttcccgaga agggtgacac
     5641 gagcgcgccg gctgcgacga tcgcacagaa ggggcgcaag ttcgtgcccg acctcgtcgc
     5701 gatcactgtc ggcgagagcg tgacgtttcc aaacaacgat ccgtttctcc acaacgtgtt
     5761 ctcgcagtcg ccgccgcgca agttcgatct cggctcgttc aagaagggcg aatcgaagga
     5821 gaaacagttt ccgaacgccg gcgtcatcga cgtctactgc aacatccatc ctgaaatggc
     5881 tgcaacgatc ctcgtgctgc cgaacaagcg gcacacgcgc accgcacccg acggcagctt
     5941 caagatcgac ggcgtaccgg cggggcgctg gacggtattc gcgtacgcgc gccgtgcgac
     6001 gaaacctgtg agcgccgccg tcaccgtcac cgacgggcaa gacgcgacgg tgaatctctc
     6061 gctcgtgcgc ggtgccgagc caccgcacgt caacaagtac ggcgagaagt acaaggaacc
     6121 gacgacaccg tacaagtagc gctacgatca aagcatgcgc gcgctgttga tcgtcgccct
     6181 cgttggctgc gcgagcggac cgaacgtcga cgcggtgatc gcgtcgcatc atcacgaccg
     6241 cacgatcgct gcgccggccg tgcgtgcgcc gcggccatgg gaggtcggac agtggacgct
     6301 gtcgaagacg tcctcgcagg ggcacgtcgg tacgatccgg cgcagcatcg tcgcgaaaga
     6361 cgcgtgcggc ttctggctcg aagtcgtcgc aacatcaccg cgcggtcgga cgatcacgaa
     6421 gacctgctac cgcagccagc tcgcgtcatc ggcgaatcca gcggccgagc tcgacgcgtt
     6481 gcaagcgatc atgatccgcc agggcggtcg caccatcgtc atcgatttcc ggaacggcaa
     6541 gaacccggcg aagaagctcc agatgcaaac gcagctgcag cagcgagtct cgctcgcgtg
     6601 gcaatcgccg tcgtccgacg agctcgccac gatcgtcgtg ccggccggtc gcttcgaggg
     6661 cgccgcccac gtcgtcgcgc ggctgtgggt cgagcaatcg atgcagaccg cacaagcgtg
     6721 gctccatccg gcggttccga tcgatggcgt ggtcaaggag agcgcgacgg atggcagcga
     6781 gctcgagctg ctcgattacg ggctcaccgg cgccaagagc gagctgccga gcttcgacga
     6841 gcagctgagc gagtctgggc tcgccgatta gtcggacttg cggtcgcgca ggtagcggat
     6901 cacgctcgcg tcgagcttgc tctggacgag acggatcaca ctctcgatct gctcgacggg
     6961 aagcttgagc cgcgcggcga gccggttgtg cgtcgcgctg accattgcgg cgcgggcgcg
     7021 ggtgagccag cgcgctgccg tcgcgcggtg cacgccgaac gcggcgccga tcgcatcgat
     7081 cgacatgtcg tcgacgatcg cctgacgcag cagcgtgcgg tcttcgggcg tcagatcttc
     7141 gatcgcctca ttgagcgcga gcgcgaactc ggtgcggtac tcggccttga gcgcttcgag
     7201 cgcggggtcg acgccgctcg tcaggtactt ctcgaggctc tgactgaacg agacctcgcg
     7261 ggtgcgcttc tttttgtgac ggatcagctc gcgcgttgcc gtgatgcgga cccaaccgcg
     7321 caacggaccg cggccgcgat agccggcgat gccgggcggc ttgccgtcct tcgtgacgag
     7381 cagctgcaag cgcagcagct gcttgacctc ttcgatctgg tcgcgctcgg cgccggtcga
     7441 agcgccagcc gcatcaacct cggtgagcac ggcgtcgaac gcggcgtgcg cagtcggttc
     7501 ctgcgcggca cacgcagccg cgatcgcgag gtcggcggcc ggtgcggcgg caatcgctgc
     7561 gacgaggtcg ctgcccgcga gccgctcgcc gatgaacgtg acgagcttgg cctcgtcgag
     7621 cttcacccgc ggccagctcg cgcgcgcggt cgcaagcagc gactcccacg cgctgagcgc
     7681 agcagtcggc gaggatgccg caagcccgct tgctgccgag agctgttgga cgcggtccgc
     7741 ggtcacgcgc tgcattctct catgtcacaa tgcctcggcg ggatggagtg cctcgacgcc
     7801 aacgtcgttc aggacctgat gtcgggggca ctcgatacgg ctcagcgtga gggcgttctc
     7861 ggccacctcg acacgtgcga ggactgccgc gagctgctcg gtatcaccgc gcgcgacacg
     7921 ttgcgcgacc agctgaagca gaacgcgtat cccgaggagg tcgcggccga cgcgcggcgg
     7981 gttgtcaacg cggccatgac gctgccgagc gccaacggcg ctaacgtcgg gatgctcgag
     8041 acggcggcgg gcaccgtggc gccgggccac gacgtcgggc tcgcggcgac gctcgccccc
     8101 gatctactcg cgacaccggt gatggggacg gaggtcgccg gcgagccgcc gacgcaggga
     8161 cgcaagctcg ggcgctacac gttgatcgaa cggctcggtg ccggcgcgat gggcgtcgtg
     8221 tggcgcgcgg aggatccgac gctgagtcgt caggtcgcgg tcaagctgtt gcgccgccat
     8281 gacgagtcac tgaccgaacg cctcgtgcgc gaggcgcagt cgatggcgca ggtcaatcat
     8341 ccgaacgtgg tgaccgtcta cgaagtcggg cagggcgtcg acggcacgac gttcatcgcg
     8401 atggagctcg tcccgggcga gagcctgcgc gcgtggcaga ccaaagcgcg ccacacgatt
     8461 ccggcgctcg tccaggcgta catcgccgcg gcacgcgggc tcgccgccgc gcactccgcg
     8521 ggcatcattc accgcgattt caaacccgat aacgtgctcg tcggtaacga cggtcgcgtg
     8581 cgcgtcaccg acttcggtct tgccgcggcg aagcagaccg tgagcgcgag cggcaggttg
     8641 cagcagatca gcgacgtgaa cctgacgacg tcgggctccg tgatggggac gccggcgtac
     8701 atggcgcccg agcaattcgt cggtggcaac gtcgatccgc gcaccgatca gttcaacttc
     8761 tgcgtcgcgc tgtacgaagc gctgtacggc gagcgaccgt tcgacggcaa gaacttccac
     8821 gagctcggcg acaacgtatg cgaaggcaag ctgaaaccgg aacccgcgaa caccgaagta
     8881 tccggcgcgc tgcgtgcgat cgtgctgcgc ggcctgtcgg tcaaacccgg cgatcggttc
     8941 gcgacgatgg acgagctgat cctcgagctc ggccgcgatc gtgcgaagcc gtggcgccgc
     9001 acgtcgatcg cgtcggccgc ggttgccgtc gcgctcggcc tcgggctcgt ctcggattgg
     9061 atcgtgcgcg atcgcgtgac gagcgagatc cggcactcgt tcgcgctcac gcgtgagcag
     9121 cagaagcgcg tcgtcaaccg actgatcgaa tcgttcaaag tcacgtcgga ggtcgcgtac
     9181 cgcgagcaag cgctacgcga ggtcgccggc catcacgatc aggccgactt cggtctcggc
     9241 gatccggaaa ccgatcaagc cgatctcgag aagctgcaca acacgttgtc gtcgacggat
     9301 tgggtgaaga acggcaccgc gacgctcgcg atcgccgact acaaaggccg cctgctgttc
     9361 acgagcgcgg gcgatacgtg gggcggcgat ctcaaggcgc tcgtgccggt gaagcgcgcg
     9421 atcgatgccg gcaagggcga cagcgtcacg gtgctgccgt acgccgatcc gggactcgcg
     9481 acgacgggca tcctcggcgc gcatccgccg acgcgaggtc tcgcagtctt gttcgagcgc
     9541 acgcttgcgc tcggcgacaa agcgagcgaa ggcagtgaag cgcgcggctt ctatctccag
     9601 ctgcaagacg gcaagcaatt gctcgacgag atttcactcg acaagcagac attgctcgga
     9661 ctcgtcgcgc tcgacggcac ggcgatcggc gagctgccgc cggcgttgat caaagccgcg
     9721 ccctccaacg aagaaagcgc agagattcgt gtcgacggtc gcgtgtatca agtgcggtcg
     9781 ctgccgatga ccgggctcgc ggcacaaggc acgatcgcgc acgtcgtgat ggcgcgtccg
     9841 ctcgacggag tgctctcgct gtttccggga gcgcgactcg tctttgcgtt gaccgctttc
     9901 ggcgctctcc tacttgcggc tacgacgtac gtaagtgcgc gcagaatcac gctg
//
LOCUS       DASUFX010000351         9174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1860560, whole genome shotgun sequence.
ACCESSION   DASUFX010000351 DASUFX010000000
VERSION     DASUFX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860560"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2046)
                     /locus_tag="VFV99_14045"
     CDS             complement(4..2046)
                     /locus_tag="VFV99_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX5060483.1"
                     /translation="MSDADPVQPAADPIRKAKLGDELNQEWLVANGLGGYASGTVLGP
                     PTRRYHGYLVAALANPSGRTMMLNTLAAQLRLHDGRRFDIGWVPPLYASHSRLELVEF
                     RLELGLPVWMFKGHGITIEKRVVVSYGTNTVLVLYRLIDGGPARIELRPGVQFRGHDE
                     PVSTIVPDAYPLWCYGSRIEVVAPTPLPSVRLLLSGQKSTFVLEPLQVPDIVYETEES
                     RGYESRGQLFSPGRFRADLEPGAHVGLFASTESWDTLLTMNCDETWAAENERRRRLLD
                     AAVEPARTGPAAELVLAADQFVIKPAGRREDHVRARAAGDEAWTVIAGYHWFTDWGRD
                     TMISLEGLTLLTGRIEEAGSILRMFAHHIRDGLVPNMFPEGSNEGLYHTADATLWFFH
                     ALDRYLRASGDRRTLIKLLPMLRDVIDHHLQGTRFNIKVDPDDGLLHQGAPGYQLTWM
                     DAKAGDWVVTPRRGKAVEINALWYNALRLMTDWLARTNDSEGAHHLGQLAERTYASFN
                     RRFWNEATGFLYDVVDGEHGNDPACRPNQLFAISLPNPVLEREYWKPVLDVVERDLVT
                     PLGLRSLSPHNPEFKPTYRGDLLARDAAYHQGTVWSWLIGPYIDALLRVHPDDVRGAQ
                     RRLAGLIAHLDEACVGNISEVFDAEAPYTPGGCIAQAWGVAELLRCLVRVTPRSPR"
     gene            complement(2043..3941)
                     /gene="treZ"
                     /locus_tag="VFV99_14050"
     CDS             complement(2043..3941)
                     /gene="treZ"
                     /locus_tag="VFV99_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX5060484.1"
                     /translation="MPRRLPIGAELSEAGVHFRVWAPVWQRVSLVLEAPTRREIALGA
                     EGNGYHSLLVDNLDAGARYWFRLGDGAQLHADPASRYQPEGPFGPSQVIDPDDTAWTD
                     EAWHGVTRPHDQIVYEMHVGTFTQEGTWTAAAAHLPFLVDVGVTTIEVMPINDFAGRR
                     GWSYDGVNLFAPTRLYGTPEELREFIDRAHAIGLAVILDVVYNHLGPAGNSLFAFAPE
                     FKNGHIDGEWGDALNFASRGVRDFFTTNAAYWISEYHFDGLRFDATQALFDPSEPHIL
                     AELSRSARAAAPHKRLWLVAENEPQDVKLLRPLDDGGCGLDALWNDDFGHTARVALTG
                     CGDGYFHDYKPTPQAFISALESAFLYQGQMYPWQRNTRGTPTRGFAPTRFVNYLENHD
                     QIANSGFGDRLIVQSDPSTFRALTAVLLLGPQIPMLFQGEETGSRKPFRFFVDHDDAL
                     NTRVRHGRAEFLAQFSRLATPEAQAALPDPAALTTFEACILDAHERDLDAPIVQLHRD
                     LIALRRSYAGFTESSLRGAVISPHAFCIRWWHEDGERLLLVNLGTTLREPILPEPLIA
                     PPEKQGWRLVWSSEHPRYGGYGTPEPFTHARLHVPGRSAVLLAPDPAKYLRIDTPPGR
                     GEKAPVDP"
     gene            4079..5104
                     /locus_tag="VFV99_14055"
     CDS             4079..5104
                     /locus_tag="VFV99_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060485.1"
                     /translation="MRRLLLVFLVATACGGKQKSTTPPPPLPEESKAQEKPAEPPKQA
                     EKAPEEPPLPKGPVDVPVPSGTVTVKLIKAGSGKKVPLKLAPKAGDKQAIELAVDFSG
                     KQTMPPELGGQTESQVNPTVIFNADVEAKEVGADGATKFEMTIKGVDAKDVAGAKNTG
                     EEVKKEFGSLAGWTVLGAVNPNGSTSDLTIHIEKPDRATQSGLEILGISLMPIWPVFP
                     TEPIGPGAKWTVTRTSKLMGKLDVTNTTDYELVSKKGKVWTLKGTTKVTGTDQEFGDE
                     GKKAKFGGIAGTGSTEATYNDGALLPTAKHSTKTDFTATVDAPEAPSKTIALVFHLEQ
                     TNAVTPK"
     gene            5169..6674
                     /locus_tag="VFV99_14060"
     CDS             5169..6674
                     /locus_tag="VFV99_14060"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5060486.1"
                     /translation="MHRVVACLLLAACGGTVAVVKTKPADEPKLDITAPWIDPEAFWI
                     RGTPTKTRPLTDALTDPNAKTIAIKNATILTATGQKIDSGTIVMEHGSITYVGADKTP
                     PQGATVIDGKGKFVSPGIIDAHSHMGVYPAPNGKAHNDGNEAVGPITAHARAEYGYWP
                     QDPQLARALAGGVTTALILPGSANLIGGRGFTVSLRPGARTAAEAAFPGAPPTVKMAC
                     GENPKRVYGEKGGPQTRMAEYAAFRAAFAEAAAYQAKKAAYENAWALWQMKKKRAEQI
                     EAKRKDNKKVPGENAPEPVPVDLRYETLAGVLRGDVLVQIHCYRADDMANLIQVADEM
                     GFRIRSFHHALEAYKIRDLLAQKNIAVNTWADWWGFKLEAYDGIPENAALLTQMGVRT
                     TIHSDSAIGGQRLNQEAAKAQAAGNRAGIKIDDDTALRWVTVNPARVLGIDGVTGTLQ
                     EGKRADVVMWSASPLSVYARAELVIVGGQIAYDKGKGLRASDFELDDGRTP"
     gene            6671..7924
                     /locus_tag="VFV99_14065"
     CDS             6671..7924
                     /locus_tag="VFV99_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5060487.1"
                     /translation="MKTLVVVALFATVALFASVASAEPIAIVGAKVYVKPGQAIDNAT
                     VVIDRGRITAVGANVKVPAGAKTIDGKDKVVTAGMIDGISSVGLVGIELEPQSVDGRF
                     PATDAVHADPVHASYQARDGFNPHDVNVAVARAGGITAVVAAPAGGLVSGQSAAFALD
                     GSAEPIGAPVAMNVEIGPNAGTAGGSRGKTIELLRELLDDARAFGRDRAGYERNAKRR
                     MIADRLDLEALQPVLARRVPMIVAAQSEADIRAALRLAKEFNVRIGIAGGTEAWRVAK
                     ELAAAKVPVLIDPTANLPSDLEASDINDDAAAVLDKAGVQVAISTLGGSWNARTLRQL
                     AGNAVAHGMSWDHALAAITNVPAVIYGLAGRGTVERGAVADLVVWSGDPLECSTGADA
                     VIINGVVQSLENHQTKLLQRYRRLP"
     gene            complement(7921..8355)
                     /locus_tag="VFV99_14070"
     CDS             complement(7921..8355)
                     /locus_tag="VFV99_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060488.1"
                     /translation="MRKLIPLIISAALGSCYTSGSVEYASSGVVDLAYVAPGVSVIAN
                     YGEPIFYSDGAYWYNAYGGWYRSPYYTGGWKYVQYPPYRISQIRAPLAYRYYRPYGYV
                     PRYRPVPANRIRTPHIYDYRSARRNYNYQPGHNPYYRDHAKY"
     gene            complement(8453..8857)
                     /locus_tag="VFV99_14075"
     CDS             complement(8453..8857)
                     /locus_tag="VFV99_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060489.1"
                     /translation="MSKLLPFLIAAALGACGGGGYYSGSVAVSASTPDLAYVAPGVSV
                     IADYDQPVFYSNGYYWYNENGYWYRSSYYTGGWTYVDSPPIAIVRIQRPYAYVHYRPH
                     NYVARNRPVHRFERPVVRDHRAARRGGYYRTR"
BASE COUNT         1660 a         2905 c         3152 g         1457 t
ORIGIN      
        1 gctctagcgc gggctcctcg gagtcactcg caccaggcag cgcaagagct cggcaacgcc
       61 ccacgcctgc gcgatgcaac cgccgggcgt gtacggcgct tccgcgtcga acacctcgct
      121 gatgttgccg acgcatgctt cgtcgaggtg cgcgatcagt ccggcgagcc tgcgttgcgc
      181 gccgcgcacg tcgtcgggat gcacgcgcag cagcgcatcg atgtacgggc cgatcagcca
      241 gctccacacc gtgccttggt gataggcggc atcgcgcgcg agcaggtcgc cgcgatacgt
      301 cggcttgaac tcgggattgt gcggcgagag cgagcgcagc ccgagcggcg tcaccagatc
      361 gcgctcgacg acgtcgagca ctggcttcca gtactcgcgc tcgagcaccg ggtttggcag
      421 cgagatcgcg aacagctggt ttggccggca cgccggatcg ttgccgtgct cgccgtcgac
      481 gacgtcgtag agaaagcccg tcgcctcgtt ccagaaccgg cgattgaatg atgcgtacgt
      541 gcgctcggcg agctgaccga gatggtgcgc gccttcgctg tcgttcgtgc gcgcgagcca
      601 gtcggtcatg aggcgcagcg cgttgtacca gagcgcgttg atctcgacgg ctttgccgcg
      661 acgaggcgtg acgacccagt caccggcctt cgcgtccatc cacgtcagct gatagccggg
      721 cgcaccttga tggaggaggc cgtcgtcggg atcgaccttg atgttgaagc gcgtgccttg
      781 caggtgatgg tcgatcacgt cgcgaagcat cggcagcagc ttgatcagcg tacggcgatc
      841 gccactcgcg cggaggtagc gatcgagcgc gtggaagaac cacagcgtcg cgtcggcggt
      901 gtgataaagg ccctcgttcg aaccctcggg gaacatgttc ggcacgaggc cgtcgcggat
      961 gtgatgcgcg aacatccgca agatcgagcc ggcttcctcg atgcggccgg tcagcagcgt
     1021 gaggccctcc agcgagatca tcgtgtcgcg gccccaatcg gtgaaccagt gatagccggc
     1081 gatcaccgtc cacgcttcgt cgcccgcggc gcgcgcgcgc acgtgatcct cacgacgacc
     1141 cgcgggtttg atcacgaact gatcggcggc gagcacgagc tcggcggcgg gacccgtgcg
     1201 cgccggctcg acggcggcat cgaggagacg gcggcggcgc tcgttctccg cagcccacgt
     1261 ctcgtcgcaa ttcatcgtga gcagcgtgtc ccacgactcg gtcgacgcga acagcccgac
     1321 gtgcgcgccc ggctcgaggt cagcgcgaaa tcggccaggg ctgaacagct gaccgcgcga
     1381 ttcgtagccg cggctctctt cggtctcgta gacgatatcg ggaacctgca acggctcgag
     1441 cacgaacgtc gacttctgtc ccgacagcag tagccgcacg ctcggtagcg gcgtcggcgc
     1501 gacgacctcg atgcgactgc cgtagcacca cagcggatat gcgtcgggca cgatcgtcga
     1561 caccggctcg tcgtgaccgc ggaactgcac accgggacgg agctcgatgc gcgccggccc
     1621 accgtcgatg aggcgataga gcacgagcac ggtgttcgtg ccgtacgaca cgacgacgcg
     1681 cttctcgatc gttatgccgt gacccttgaa catccacact ggcaagccaa gctcgagccg
     1741 gaattcaacc agctcgaggc ggctgtggct cgcgtagagt ggcggcaccc agccgatgtc
     1801 gaaccgccgg ccgtcgtgca gccggagctg cgcggcgagt gtgttcagca tcatcgtacg
     1861 acccgacggg ttcgcgagcg cggcgaccag gtagccgtga taacggcgcg tgggtggacc
     1921 gagcaccgtg cccgacgcgt agccgccgag accgttcgcg acgagccact cctggttgag
     1981 ctcgtcgcca agcttcgcct tgcgaatcgg atctgcagcg ggttggacgg gatctgcgtc
     2041 gctcatggat cgaccggtgc cttctcgccg cggccaggcg gcgtgtcgat acgcaaatac
     2101 ttggcaggat caggtgcgag cagcacggcg ctccggccag gcacgtggag tcgcgcgtgc
     2161 gtgaatggct cgggcgtacc gtaaccgccg tagcgcggat gctcactcga ccacacgagg
     2221 cgccagccct gcttctcggg cggtgcgatc agcggctcgg gcaggatcgg ttcgcgcagc
     2281 gtcgtgccga gattgacgag tagcagccgt tcgccgtcct cgtgccacca gcgaatgcag
     2341 aatgcgtgcg gcgaaatcac ggcgccgcgc agcgagctct ccgtgaagcc ggcgtagcta
     2401 cggcgcagcg cgatcaagtc gcgatgcagc tgcacgatcg gcgcgtcgag atcgcgttca
     2461 tgtgcgtcga ggatgcacgc ttcgaacgtc gtgagcgcgg ccggatcggg tagtgcggct
     2521 tgtgcttccg gcgtcgcgag ccgcgagaac tgcgcgagga actcggcgcg gccgtggcgg
     2581 acgcgcgtgt tcagcgcgtc gtcgtgatcg acaaagaagc gaaacggttt tcgcgatccc
     2641 gtctcctcgc cctgaaacag catcgggatt tgcgggccga gcagcagcac cgcggtcagt
     2701 gcgcggaacg tcgacggatc gctctgcacg atcagccgat cgccgaagcc actgttcgcg
     2761 atctgatcgt ggttctcgag atagttcacg aaccgtgtcg gcgcgaagcc gcgggtcggc
     2821 gtaccgcgcg tgttgcgctg ccacggatac atctggccct ggtagaggaa cgcgctctcg
     2881 agtgccgaga tgaacgcctg cggcgtcggt ttgtaatcgt gaaagtagcc atcgccgcag
     2941 ccggtcagcg cgacgcgcgc cgtgtgtccg aagtcgtcgt tccacagcgc atcgaggcca
     3001 caaccaccat cgtcgagcgg ccgcaacagc ttcacgtcct gcggctcgtt ctcggcgacg
     3061 agccagagcc gcttgtgcgg tgccgcggcg cgtgcgctgc gcgatagctc ggcgaggatg
     3121 tgcggctcgc tcgggtcgaa cagcgcttgg gtcgcatcga agcgcaggcc gtcgaagtgg
     3181 tactcgctga tccagtacgc cgcgttcgtc gtgaagaaat cgcggacgcc gcggctcgcg
     3241 aagttcagcg cgtcgcccca ttcgccgtcg atgtggccat ttttgaattc gggcgcgaac
     3301 gcgaatagcg aattgcccgc cggacccaag tggttgtaga cgacgtcgag gatcactgcg
     3361 aggccgatcg cgtgcgcgcg atcgatgaat tcgcgaagct cctcgggggt gccgtagagt
     3421 cgcgtcggcg cgaacaagtt gacgccgtcg tagctccagc cgcggcggcc ggcgaagtcg
     3481 ttgatcggca tcacctcgat cgtcgtaacg ccgacgtcga cgaggaacgg gagatgcgcg
     3541 gcggcggccg tccacgttcc ctcctgcgtg aacgtaccga cgtgcatctc gtaaacgatc
     3601 tgatcgtgcg gccgcgtgac gccgtgccag gcctcatcgg tccatgcggt gtcgtcggga
     3661 tcgatcacct gcgacggacc aaacggacct tcgggctggt agcgcgatgc cggatccgcg
     3721 tgaagctgtg caccgtcgcc gaggcggaac cagtagcgtg cgccagcgtc gagattgtcg
     3781 acgagcagcg agtgatatcc gttgccttcc gcgccgagcg cgatttcgcg acgcgttggt
     3841 gcttcgagga cgagcgagac gcgttgccac acgggcgccc agactcgaaa gtgaacgccc
     3901 gcctccgaca gctctgcgcc gatcggtagt cttcgcggca cgcactgtag cggtgcagca
     3961 cgtgtgccat gaaatctcgc tccttacgtc ggagttgcct gcgccaggac gcgcagcccc
     4021 gcacgtcgag cgggcacgcg cacgtgagag atggtcgtgc gcgtggtata gccccgcaat
     4081 gcgtcgactg ctcctcgtgt tcctggtcgc taccgcctgc ggtggcaagc aaaagtccac
     4141 cacgccaccg ccgccgctcc cggaggaatc gaaggctcaa gagaaaccgg cggagccgcc
     4201 gaagcaggcc gagaaggccc ccgaggagcc gccgcttccg aaggggccgg tcgacgtgcc
     4261 ggtgccgtcg ggtaccgtca cggtgaagct gatcaaggcc ggctcgggca agaaggtgcc
     4321 gctcaagctc gcgccgaagg cgggcgacaa gcaggcgatc gagctagcgg tcgatttctc
     4381 gggcaagcaa acgatgccgc ccgagctcgg cggtcagacc gagagccagg tcaatccaac
     4441 ggtcatcttc aacgctgacg tcgaggcgaa ggaagttggc gccgacggcg cgacgaagtt
     4501 cgagatgacg atcaagggcg tcgatgcgaa ggacgtcgcc ggcgcgaaga acaccggtga
     4561 agaggtcaag aaggagttcg gttcgctcgc gggctggacc gtgctcggcg ccgtgaaccc
     4621 gaacggttcg acgtcagacc tgacgattca catcgagaag ccggatcgcg cgacgcagag
     4681 tggcctcgag atcctcggca tctcgctgat gccgatctgg ccggtcttcc cgaccgagcc
     4741 gatcggtcct ggcgcgaagt ggacggtcac gcgcacgtcg aagctcatgg gcaagctcga
     4801 tgtcacgaac accaccgact acgagctggt ctcgaagaag ggcaaggtat ggacgctcaa
     4861 gggcaccacg aaggtgaccg gcacggacca ggaattcggc gacgaaggca agaaggcgaa
     4921 gttcggtggc atcgccggca ccggctcgac cgaggcgacg tacaacgatg gcgcgctgct
     4981 gccgactgcg aagcactcga cgaagacgga cttcaccgcg accgtcgatg cgcccgaggc
     5041 gccgtcgaag acgatcgcgc tcgtgttcca tctcgagcag acgaacgcgg tcacgccgaa
     5101 gtagccgtcg cgcacgtcgc agcctgtcgc acgacgcttg agggccgagt ctgggttaca
     5161 accgccccat gcacagggtg gttgcatgct tgctgctcgc tgcgtgcggc ggcaccgtcg
     5221 cggttgtcaa gacgaaaccg gcggatgagc cgaagctcga catcacggcg ccgtggatcg
     5281 atccagaggc gttctggatt cgcggcacgc cgacgaagac gcggccgctg accgacgcgc
     5341 tcactgatcc gaacgcaaag acaatcgcga tcaagaatgc gacgatcctg accgcgaccg
     5401 gtcagaagat cgacagcggc acgatcgtga tggagcacgg ctcgatcacg tacgtcggcg
     5461 cagacaagac gccgccgcag ggcgcgacag tgatcgatgg caaaggcaag ttcgtctcgc
     5521 ccggcatcat cgatgcgcat tcgcacatgg gcgtgtatcc ggcgccgaac ggcaaggcgc
     5581 acaacgacgg caatgaagcg gtcggcccga tcaccgcgca cgcgcgcgcc gagtacggct
     5641 actggccgca ggatccgcag ctcgctcgcg cgctcgccgg aggggtgacg accgcgctga
     5701 tcctgccggg ctcggcgaac ctgatcggcg gccgcggttt cacggtatcg ctgcggccgg
     5761 gcgcacgcac cgcggcggaa gcggcatttc ccggtgcgcc accgacggtg aagatggcgt
     5821 gcggcgagaa tccgaaacgc gtgtacgggg agaagggcgg accgcagacg cgcatggccg
     5881 agtacgcggc gtttcgcgcg gcgttcgccg aggcggcggc ataccaggcg aagaaggcgg
     5941 cgtacgaaaa cgcgtgggcg ttatggcaga tgaagaagaa acgcgccgag cagatcgaag
     6001 cgaagcgcaa ggacaacaag aaggtccctg gtgagaacgc gccggagcca gtgccggtcg
     6061 atctacgcta cgagacgctt gccggcgtgc tgcgcggcga cgtgctcgtg cagatccact
     6121 gctatcgcgc cgacgacatg gcgaacttga tccaggtcgc cgacgagatg ggcttccgga
     6181 tccgctcgtt ccatcacgcg ctcgaggcgt acaagatccg cgacctgctc gcgcagaaga
     6241 acatcgcggt caacacctgg gccgactggt ggggcttcaa gctcgaggcg tacgacggga
     6301 ttcccgagaa tgcagcgctg ctgacgcaaa tgggcgtgcg cacgacgatc cactcggact
     6361 cggcgatcgg tggccagcgg ctcaatcaag aagcggcgaa ggcgcaggcg gcgggcaatc
     6421 gcgctggtat caagatcgac gacgacaccg cgctgcgctg ggtgaccgtg aatcccgcgc
     6481 gcgtgctcgg catcgacggt gtcaccggca cgctgcaaga aggcaagcgc gccgacgtgg
     6541 tgatgtggag tgcatcgccg ctgtcggtct acgcgcgtgc cgagctcgtg atcgtcggcg
     6601 gccagatcgc ctacgacaaa ggcaagggcc tgcgcgcgag cgacttcgag ctcgacgacg
     6661 ggaggacgcc atgaagaccc tcgttgtcgt cgcgctgttc gcgaccgtcg cgctgttcgc
     6721 gagcgtcgcg agcgcggagc caatcgcgat cgtcggcgcg aaggtctacg tcaaacccgg
     6781 gcaggcgatc gacaacgcga cggtcgtgat cgatcgcggc cgcatcacag cggtgggcgc
     6841 caacgtgaag gtgccggcgg gtgcgaagac gatcgacggc aaggacaaag tcgtgaccgc
     6901 aggcatgatc gacggcatct cgtccgtcgg cctcgtcggc atcgagctag agccgcaatc
     6961 cgtcgacggc cggtttcctg ccaccgatgc cgtgcacgct gatccggtgc acgcgtcgta
     7021 tcaagcgcgc gatggcttca atccgcacga cgtcaacgtc gcggtcgcgc gtgccggtgg
     7081 catcaccgcc gtcgtcgctg cgccggccgg cgggctcgtg tccggtcagt cggcagcatt
     7141 cgcgctcgac ggcagtgccg agccgatcgg tgcgccggtc gcgatgaacg tcgagatcgg
     7201 tccgaacgcg ggaaccgcgg gtggttcacg tggcaagacg atcgagctcc tgcgcgagct
     7261 gctcgacgac gcgcgtgcat tcggtcgcga tcgtgccggc tacgagagga atgcgaagcg
     7321 acgcatgatc gccgatcgcc tcgatctcga agcgctgcag cccgtgctcg cgcgtcgggt
     7381 accgatgatc gtcgccgcgc agagcgaagc ggacattcgc gccgcgcttc gtctcgcgaa
     7441 agaattcaac gtgcgcatcg gcatcgccgg cggcaccgaa gcatggcgcg tcgcgaaaga
     7501 gctcgcggct gcaaaggtgc cggtgttgat cgatccgacc gcgaacttgc cgtcggatct
     7561 cgaagcgagc gacatcaacg acgacgcggc cgccgtgctc gacaaagccg gtgtgcaggt
     7621 cgcgatctcg acactcggtg gttcgtggaa tgcgcggacg ctgcgtcagc tcgcgggcaa
     7681 cgcggtcgcg cacggcatgt catgggatca cgcgctcgcc gcgatcacga acgtgcccgc
     7741 ggtgatctac ggtctcgcgg gtcgcggcac cgtcgagcgc ggtgccgtcg cggatctcgt
     7801 ggtgtggagc ggcgatccgc tcgagtgctc gacgggcgcg gacgcggtga tcatcaacgg
     7861 cgtcgtgcag tcgctcgaga atcaccagac gaagctgctg cagcgttatc gccgcttgcc
     7921 ttagtacttc gcgtggtcgc ggtaatacgg gttgtgaccg ggctggtagt tatagttccg
     7981 gcgtgctgac cgataatcgt agatgtgcgg cgtgcggatg cgattggcag gcaccggccg
     8041 gtagcgcggc acgtagccgt acggccggta gtagcgatac gcgagcggcg cgcgaatctg
     8101 cgagatgcga tacggcgggt actgcacata cttccaaccg ccggtgtaat agggcgagcg
     8161 gtaccagccg ccgtacgcgt tgtaccaata cgcgccgtcg gaatagaaaa tcggttcgcc
     8221 gtagttggcg atcacgctga caccgggcgc gacgtacgcg aggtcgacca caccggagct
     8281 ggcgtactcg actgagccgg acgtgtagca gctgccgagc gctgccgaaa tgatcaacgg
     8341 gatgagcttc cgcataagtc ctcgtcccgt tcaaaaagca tcgcgcatgc cgcagccccg
     8401 gatctccgtc ggagatccga gactcactgt cgcgtgctcg cggtagcgcg ggttagcgcg
     8461 tgcggtagta accaccgcgt cgggccgcac gatggtcgcg caccaccggt cgctcgaagc
     8521 ggtgcactgg acggttacgc gccacgtagt tgtgcgggcg ataatgcacg tacgcgtacg
     8581 ggcgttggat gcgcacgatc gcgatcggtg gcgagtcgac gtacgtccag ccgcccgtgt
     8641 aatacgacga ccggtaccag tagccgttct cgttgtacca gtagtagccg ttcgaataga
     8701 acaccggctg gtcgtagtcg gcgatgaccg agacgcccgg cgcgacgtac gcgaggtccg
     8761 gcgtgctcgc cgagaccgcg acgctacccg agtagtagcc accgccgccg caagcaccga
     8821 gtgccgctgc gatcagaaag ggaagtagct tcgacatgag atcctccacc taggaggaaa
     8881 gcatcgagcg tgccgccgta cgcgatcggc gaattcccga tggcgcgcgc taccgcaacg
     8941 agatgcaggt gctcggcgtg gccgcgatct gcgcacttgc tgcggccgcg cacactgcgc
     9001 ccgactcgat gcgcacagcc atcgctgcac gagacggtgc gttgctcagt cgacgtcgac
     9061 gtcgaaatcg aaatcgaagc ggccgggatt gacttgaacg ttcacgtcgt cgctgtcgct
     9121 atcgctgtca tcgtcgctgt cgctatcgct gtcctcgtcg tccatgccgt tcca
//
LOCUS       DASUFX010000352         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_749574,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000352 DASUFX010000000
VERSION     DASUFX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749574"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..543)
                     /locus_tag="VFV99_14080"
     CDS             complement(58..543)
                     /locus_tag="VFV99_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060490.1"
                     /translation="MKRSLLLLIALAAPRVADAAPCDTLPNPIYLQVGDTQNNLIKRL
                     ARALRDNTDKPITLVWTTSGSCTNIQAIYTRVAIAANTNMQYVPSIAEDATWTPSSAT
                     LTCTIPTGGKVPDVANSALFNSACTTEQPPATVNLTYGPVQAYVMAVPEASSQTAITF
                     E"
     gene            884..2833
                     /locus_tag="VFV99_14085"
     CDS             884..2833
                     /locus_tag="VFV99_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5060491.1"
                     /translation="MRWQINLFALSTVACASLVRVAAAQDAPEPPPPPVVDTPEPPPP
                     QELTDEQLARLSEGEAIEIFDERPDKPFDRDTEVRLTGEQLAARGAVDLATALALLPD
                     VSVQEAGRGGSTVIIRGASKGQVSVLIDGVLVSDPYYGTFDLTSIPITDIVQIRLATT
                     PQSPIDGVGGPGGVIEVHTRDAIGPQLVIARMASDTLPSFGMSGTARAALSKRLALRI
                     AASGVGGGRSLTLAAPFDMISESRRDAAGSLRLEYRADKRRIVLDGFANDRHYIVPPS
                     ETTTALQLIDRENNARAAAKLDDTIDDYQIQVQGAFQYLLRHTRSFSDPTFGFESAAE
                     KLHANRTGGMALVTHPIGKDARWAASATVEHERADVSAGSLMMPIVARGNVTLAEVAG
                     DLQYEHRTVRLDGAVGVAFPFGTDANPWPEAKLVGKWRPNFGNLEVTATGARKGRVPS
                     LRERFQPGLGDPDLAPEIINHGEVRAIEHIANRIHIELATFARYQQGTIIGTTQPPDV
                     GKLINLGTVTVGGIDVLARVRIHPKIEVGGAYNYVRAHSDEKGDDPLSHLPHHKGEAW
                     VQVAPITGLTVMTRGNFFGESFINQMTRIPPYTAFEVSASWQLAKEYLAVLRGTDLGD
                     SRPLIRPGVYGPGRTISAVLQATWD"
     gene            2910..3167
                     /locus_tag="VFV99_14090"
     CDS             2910..3167
                     /locus_tag="VFV99_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060492.1"
                     /translation="MTGESGTKADAAAGGDGPAAGTFGAACTTVSDQSTECMSGVCTN
                     TFDQLGHPVCSQKCTVLQGTDPTCPMGPSNQKCNMQGYCKP"
     gene            complement(3169..3870)
                     /locus_tag="VFV99_14095"
     CDS             complement(3169..3870)
                     /locus_tag="VFV99_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060493.1"
                     /translation="MAARVLVVDDERDVCRLLTFSLEQAGFEVSSAGTGAEALLSIGR
                     KPPAVIVLDVGLPDLSGVDLCRRLRADQDIGDVGIVMLTALGSREDRIVGLEAGADDY
                     VVKPFDVQEVVLRVRALSRRISERDRAKGASAGQPAQLLRSGTLTVDPVTFEVRGGDR
                     LLALRPLELRLLMTLMSEPGRVYSREELLRSVWEIEHGSVRTVDVHVRRLRINLGEWA
                     DQIETVPGFGYRAKR"
     gene            3965..>4382
                     /locus_tag="VFV99_14100"
     CDS             3965..>4382
                     /locus_tag="VFV99_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060494.1"
                     /translation="MTISFRAKLLASHAVVALVVGLVTLIVVDRQVSRSMEHQIDQRL
                     EAQARAVATWMERAAHPAQLARRLAGVVDARVTILDKHGIAVGESHAEANAKPAMDSE
                     GDPPEVLAARDGKLGRETRFSAYEGQQVRYVAVPAPQ"
BASE COUNT          792 a         1468 c         1417 g          705 t
ORIGIN      
        1 ctcgtcgctc cacggctcga ccatgccggc catccccatg ccgaatacga agtacgccta
       61 ttcgaacgtg atcgcggtct gcgacgacgc ttccggcacc gccatcacgt acgcttgcac
      121 cgggccgtac gtgagattca cggtcgcggg cggctgctcg gtcgtacacg cgctgttgaa
      181 cagcgcggag ttcgcgacat cgggcacctt gccgcccgtc gggatcgtgc aggtgagcgt
      241 tgccgacgat ggcgtccacg tagcgtcctc ggcgatcgac ggcacgtact gcatgttcgt
      301 gttcgccgcg atcgctacgc gcgtgtagat cgcctggatg ttcgtgcacg agccgctcgt
      361 cgtccacacc agcgtgatcg gcttgtcggt attgtcgcgc aatgcccgcg cgagtcgctt
      421 gatcaggttg ttctgggtat cgccgacctg gaggtagatc gggttcggca acgtgtcgca
      481 cggcgcggcg tccgcgacgc gaggcgctgc cagtgcgatc aacagcaaca ggctccgctt
      541 cattggaaca tcctcggcgt cgcgcgcgag agcgtccaac cacaaaccga tgacagatcg
      601 agtgcaggcg cgtgcgatga cactgcgaag atctcgcgat gacagagcga tgaactgtgg
      661 cttgccgcct ggggccacag gtgaggaccg tggtcccgca gccgtccgga ggcgacggct
      721 gtccttgacg acaagtacgg gatcgccgac cgtgatcatg ggtgaagcgt cacgttcgta
      781 acgccactgt tacgtgtgcg gacggccggc agcgaccgcc atccgtcgcg caaagcggca
      841 cgcagcctgc aattcgtccc cggggcccgc ggccgtactt gcaatgaggt ggcaaataaa
      901 ccttttcgcg ctctccactg tcgcgtgcgc gagcctcgtc cgcgttgctg ctgcgcagga
      961 cgcgccggag cccccacctc ctccggtcgt ggacacgccg gagccgcccc ctccgcagga
     1021 gctgacggac gaacagctcg ccaggctctc cgagggcgaa gcgatcgaga ttttcgacga
     1081 gcgccccgac aagccattcg atcgcgacac cgaagtgcga ttgaccggcg agcagctcgc
     1141 cgcacgtggt gccgtcgacc tcgcaaccgc actcgcgctg ctgccggacg tctcggttca
     1201 ggaagcaggc cgcggcggat cgacggtcat cattcgtggt gcgagcaagg gccaggtcag
     1261 cgtcctgatc gatggcgtgc tggtcagcga cccgtactac ggcacgttcg atctgacctc
     1321 gatcccgatc accgacatcg tgcagatcag gctcgcgacg acgccgcagt cgccgatcga
     1381 cggcgtcggt ggcccgggcg gtgtcattga agtccacacg cgcgacgcga tcggcccaca
     1441 gctcgtcatc gcacgaatgg caagtgacac gctgccttcg ttcggcatgt cgggaaccgc
     1501 acgagccgca ctgtcgaagc gcctagcgct acgcatcgcc gcgtcgggcg tcggcggcgg
     1561 acggtcactt acgctcgcgg cgccgttcga catgatcagc gagagccgcc gcgatgccgc
     1621 tggttcgttg cggctcgagt accgcgccga caaacgccgc atcgtgctcg acgggttcgc
     1681 caacgatcgg cactacatcg tcccgccaag cgagactacg acggcgctcc agctgatcga
     1741 tcgcgagaac aacgcccgcg cggccgcaaa gctcgacgac acgatcgatg actatcagat
     1801 ccaggttcag ggcgcgtttc agtacctgct gcgccacacg cgcagcttca gcgatccgac
     1861 gtttggcttc gaaagcgccg ccgagaagct gcacgcgaac cgcaccggtg gcatggcgct
     1921 cgtgacgcat ccgatcggca aggacgcgcg ctgggccgcg tcggccaccg tcgaacacga
     1981 acgcgccgac gtgagtgctg gctcgctcat gatgccgatc gtcgctcgcg gcaacgtcac
     2041 gcttgccgag gttgcgggtg acttgcaata cgagcatcgg accgtgcggc tcgacggcgc
     2101 cgtcggcgtg gcgtttccgt tcgggaccga tgcgaacccg tggccggagg caaagctcgt
     2161 cggaaaatgg cgaccgaact tcggcaacct cgaggtcacc gccaccggcg cacgcaaggg
     2221 gcgcgtcccg agcctgcgcg agcggtttca gcccgggctc ggtgatccgg atctcgcacc
     2281 cgaaatcatc aaccacggcg aagtgcgcgc gatagagcac atcgcgaatc gcatccacat
     2341 cgagctcgcg acgttcgctc gttaccagca gggaacgatc atcggcacga cccagccgcc
     2401 cgacgtcggc aagctcatca acctgggcac ggtgacggtg ggcggcatcg acgttctcgc
     2461 ccgcgttcgc attcatccga agatcgaggt cggcggcgcc tacaactacg tgagagcgca
     2521 cagcgacgag aagggcgacg atccgctgtc gcatcttcca caccacaagg gcgaggcctg
     2581 ggtgcaagtc gcaccgatca ccggcttgac cgtgatgacg cgcggcaact tcttcggcga
     2641 gagcttcatc aaccagatga cccggattcc gccctacact gcattcgagg tctcggcatc
     2701 ctggcaactc gcgaaggagt acctcgccgt cttgcgcggc accgatctcg gggattcgcg
     2761 tccgctgatc cgccccggcg tctatggccc cggacgaacc atttctgcgg tgctgcaagc
     2821 cacgtgggac tagcagccac ttcgactacc aaggagcaac gaccatgact cgcattattc
     2881 tcatcgcact catgttctca gccgcatgca tgactggaga aagtgggacg aaagcagatg
     2941 cagcggccgg aggagacgga ccggctgcgg gaaccttcgg tgccgcgtgc acgaccgtca
     3001 gcgatcagtc gaccgagtgc atgagcggtg tgtgcacgaa cacgttcgat cagctcggcc
     3061 acccggtttg ctcgcagaag tgcacggtgc tgcagggcac ggatccgacg tgcccgatgg
     3121 gcccgtcgaa ccagaagtgc aacatgcagg gctactgcaa gccctgagtc agcgcttcgc
     3181 gcggtacccg aagccgggaa cggtctcgat ctgatcggcc cactcgccga ggttgatgcg
     3241 caagcgacgc acgtgaacgt cgacggtgcg cacactgcca tgctcgattt cccacaccga
     3301 gcgcaacagc tcctcgcggc tgtacacgcg gccgggctcg ctcatcagcg tcatcagcag
     3361 cctgagctcg agcggccgca gcgcgagcag cctgtcgccg ccacggactt cgaacgtgac
     3421 cggatcgacc gtgagcgtac cggagcggag gagctgggcc ggttgtcccg cggacgcacc
     3481 cttggcgcga tcgcgttcgc tgatccggcg cgatagcgcg cgtacgcgga gcacgacctc
     3541 ctggacgtcg aacggtttga cgacgtagtc atcggcacca gcctcgaggc caacgatgcg
     3601 atcctcgcgc gagccaagcg ccgtgagcat cacgatgccg acgtcaccga tatcctgatc
     3661 ggcgcgcagg cggcggcaga ggtccacacc cgacagatcg ggcaagccga cgtcgagcac
     3721 gatcacggcg ggcggcttgc ggccgatcga cagcagcgcc tcggcaccgg tgcctgcact
     3781 ggatacttcg aaaccggctt gctcgaggct gaacgtgagc aggcggcaga cgtcgcgctc
     3841 gtcgtcgacc acgaggacac gtgctgccat gacgcgattt tccactatcc gcgcgcgaag
     3901 cgcgaggcgt gtcatcgagc tgtcttcgcg ctgtcacgac caaaggcgtc tactatcagg
     3961 catcgtgacg atctcctttc gcgccaagct gctcgcgtcc catgcagtcg tggcgctcgt
     4021 cgttggcctg gtcacgctga tcgtcgtcga tcgccaggtt tcgcgaagca tggagcacca
     4081 gatcgatcaa cggctcgaag cgcaggcgcg tgcggtggcg acctggatgg agcgcgccgc
     4141 gcaccccgca caacttgcgc gccggctcgc gggcgtcgtc gacgcgcgcg tgacgatcct
     4201 cgacaagcac ggcatcgcgg tcggcgaatc gcatgccgaa gcgaacgcga agccggcgat
     4261 ggactccgaa ggtgacccgc cggaagtgct cgccgcgcgc gacggcaagc tcggccgcga
     4321 gacccggttc tcggcgtacg agggtcagca ggtgcgctac gtcgcggtgc cggcgccgca
     4381 ag
//
LOCUS       DASUFX010000353         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1443290, whole genome shotgun sequence.
ACCESSION   DASUFX010000353 DASUFX010000000
VERSION     DASUFX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443290"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFV99_14105"
     CDS             <1..399
                     /locus_tag="VFV99_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060495.1"
                     /translation="EDLAIELLQQLDLSFLAAALLRDPGLTERVTPSVSGVVTRATIE
                     RMIGPLGIQTGWIACVDVRRNARAHEGVLQGSDGSLYPVEGVLSVEDMPFDDNGEPFD
                     VVVEVDELPDGSLEFADRFSDDAFAVHAGM"
     gene            471..1961
                     /locus_tag="VFV99_14110"
     CDS             471..1961
                     /locus_tag="VFV99_14110"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX5060496.1"
                     /translation="MAVGDEADDGRAREPGERVAVLEAALAERDAKLAERDAQLATLT
                     EQIAKLSAQIEVLTELLNRNSKNSHLPPSSDGPGASGGSASGRRPKSERKRGGQKGHR
                     GSYRELLPAERVDTFVDLFPPVCLGCACELPRVIDVAACRYQQLELRDHRPHLTEWRR
                     HEVDCERCGASTRAAYDSSQIPSSAFGPCLTAVVAMLTGAYHLSRRKTQKLLAELFGI
                     AVSLGAISAMEQRASEALASAYEEALREVQYAEVKHTDGTTWARAGKLMSLWTLATAA
                     ATVYRIFADGCRDTIRPMFGPRLGILVSDRATVFDFWTMALRQICHAHLLRKFVAFSE
                     RDGPAGVLGHELLQLSSLVFEYWHGFKQGHLNRDELQLWMRPVQRDFERLLERGAAAD
                     IERLSGSCADILAHRDALWTFVTQRGVEPTNNHGELELRDFVLWRKRSFGTQSERGER
                     FAERVMTAVRTARKQGKDVLDFVVRSVIAHLDGSTPPLLIDAAVPA"
BASE COUNT          347 a          626 c          688 g          339 t
ORIGIN      
        1 gaagacttag cgatcgaact tcttcagcag ctggacctaa gcttccttgc agcagcgtta
       61 cttcgagacc ctggtttgac agagcgagtc actccaagcg ttagtggagt cgtaacgcgg
      121 gcaactatcg agcggatgat cgggcctctc ggcattcaga ccggctggat cgcatgcgtc
      181 gacgtgcgcc gaaacgcaag agcccatgaa ggagtgctgc aaggaagcga cggttcgctg
      241 tatcccgttg aaggcgtgct ttccgttgaa gacatgccat ttgacgacaa tggggagccg
      301 ttcgacgtcg tcgttgaagt cgacgagctt cccgacggca gcctcgagtt cgccgatcgc
      361 ttcagcgatg acgcgtttgc ggtgcacgca ggcatgtaac cgttcagccc tcggaatttc
      421 cgtgcacgag acggatggac atggcgatcc gatcgtgatc taagctgcat gtggcggttg
      481 gcgacgaggc tgacgacgga cgtgctcgcg agccagggga gcgcgttgcg gtgctcgagg
      541 cggctctcgc ggagcgtgac gcgaagctcg ccgagcgcga cgcgcagctc gcgacgctga
      601 cggagcagat cgccaagctg tcggcgcaga tcgaggtgtt gaccgagctg ctgaaccgca
      661 actcgaagaa ctcgcaccta ccgccatcga gcgacgggcc gggcgcgagc ggtggttcgg
      721 caagcgggcg tcggccgaag tcggagcgca agcgcggcgg tcagaagggc caccgcggct
      781 cgtatcgcga gctgctcccc gccgagcgcg tggacacgtt cgtggatctg ttcccaccgg
      841 tatgcctggg ctgcgcgtgc gagctgccgc gggtcatcga tgtcgccgcg tgccggtacc
      901 agcagctgga gctgcgcgat caccgaccgc acttgaccga gtggcgccgc cacgaggtgg
      961 actgcgagcg ctgcggtgcc tcgacccgag cggcctacga tagttcgcag atccccagct
     1021 cggcgttcgg gccttgtctc accgcggtcg tcgcgatgct gaccggcgcc taccatctga
     1081 gccggcgcaa gacgcagaag ttgctcgccg agctgttcgg gatcgcggtc tcgctcggcg
     1141 ccatcagcgc gatggagcag cgtgccagtg aagcgctcgc gtcggcgtac gaagaggcgc
     1201 tgcgtgaggt gcaatacgcc gaggtcaagc acaccgacgg cacgacttgg gcgcgcgccg
     1261 gcaagctgat gtcgctatgg acgttggcca cggcggcggc gacggtctat cgaatcttcg
     1321 ccgatggatg ccgcgatacc atccggccga tgtttggtcc gcggctgggc atcctcgtga
     1381 gcgatcgcgc cacggtcttc gacttctgga cgatggcgct acggcaaatc tgccatgcgc
     1441 acttgctccg caagttcgtc gccttctcgg agcgcgatgg tccggccggc gtgctcgggc
     1501 acgaactgct gcaactgagc tcgctcgtat tcgagtactg gcacggcttc aagcaaggcc
     1561 acttgaaccg agacgagctg cagctgtgga tgcgcccggt gcagcgtgac ttcgagcggc
     1621 tgctcgagcg cggcgccgcg gccgacatcg agcggctgtc gggctcgtgc gccgatatcc
     1681 tcgcgcaccg cgacgcgctg tggaccttcg tgacgcaacg gggtgtcgag ccgaccaaca
     1741 accacggtga gctcgagctg cgagatttcg tgctgtggcg caagcgcagc ttcggcaccc
     1801 agagtgaacg cggcgagcgc ttcgccgagc gcgtgatgac cgcagtgcgg acagcgcgca
     1861 agcagggcaa ggacgtactc gacttcgtcg tccgcagcgt gatcgcccac ctcgacggct
     1921 cgacgccgcc actgctcatc gacgcggcgg tgccggcctg atcaatacgc gctcacgcgg
     1981 accgaaccac ggcggggaag
//
LOCUS       DASUFX010000354         8959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2001322, whole genome shotgun sequence.
ACCESSION   DASUFX010000354 DASUFX010000000
VERSION     DASUFX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001322"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV99_14115"
     CDS             <1..261
                     /locus_tag="VFV99_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060497.1"
                     /translation="DPATGLGTRYTEASFIATMRTGKKPNGTPIMPPMPSDVYKNMTD
                     DDLGAIFAYLQTIPAIKNQVPDPLAPQQAQLVANPQQIASQQ"
     gene            258..812
                     /locus_tag="VFV99_14120"
     CDS             258..812
                     /locus_tag="VFV99_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5060498.1"
                     /translation="MSRVDLFTVIHKGIRSLLFELAREAARVDLTSTHAVDGLVARVE
                     RTLGFLDEHARLEDMHVFTALRALGGELADELNAEHRSLEVVQLEVERAADALALAQL
                     AGREEAGAHLARLINHLVAVQLIHMNREETEVNATLWAGLPDSELGAIRTRLKNTLSP
                     ERFDEWMQIVGPALNPVEHRLVIG"
     gene            823..1317
                     /locus_tag="VFV99_14125"
     CDS             823..1317
                     /locus_tag="VFV99_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060499.1"
                     /translation="MSASYPPLPPELVTFAEGGVSILVGTCNRELVPDCVRGMGVRVW
                     PGASGLTVLLPAATAAASIANVRDNPRLAVTLSHIPSHRTIQVKGKVIAVRDGDDADR
                     ELATRYREKLADDLAFVGQPAANTLRLGIWPCHAVDLEIEVVFAQTPGPVAGVKMPLQ
                     TGRL"
     gene            1314..2681
                     /locus_tag="VFV99_14130"
     CDS             1314..2681
                     /locus_tag="VFV99_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5060500.1"
                     /translation="MIRLDALDQCFHGIVPGVLATCDREAIPNVSYISQVHLIDEQHL
                     ALSCQFFNKTRQNLDENPLATLELYDPLTFEAYRLRLRFLRSESTGPLFDSMAVRILA
                     IASHTGMAGVFKLRSADVFEVLSIEQREGYIEPPAAGERLVRSLPNGPLTELRGLQLV
                     SERVARASDLDSLLTGALAALDEVFGFTHGMIMMPEHGKKLVTVASHGYGDAAIGAEV
                     GCGEGMIGNVADTKRLVRIASASAELRYGRAIREQYAHTGSGVGAEIPLPRLADAESQ
                     MAIPLLVQDRLVGVLALECRSRLNFEQWHEAFLQVLANQIASGIERMAEREDDGAMSL
                     PAAAPPKRVPAARTLNFTYYRNDDCVFVDGEYLIRNVPGKILWKVLRAYEAEKRTDFS
                     NRELRLDPTLGLPPIKDNLESRLILLRKRLEQKCPDVRLVPTRRGRFSLCIGCELELV
                     ERETA"
     gene            complement(2915..4180)
                     /locus_tag="VFV99_14135"
     CDS             complement(2915..4180)
                     /locus_tag="VFV99_14135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5060501.1"
                     /translation="MIVPIGHDQSIRRMPWVTIAIIALCTLMQLYATLGAPSEDQLAN
                     ELIALTDRYTDMAAENEDGQLTPEQEQEHDRQVEAIANKLPAYRFGYKTHEGLQTGLI
                     TSAFVHGGWMHLIGNMLFLWLAGSALEDRYGRVRFTLFYLLGAAASAFAYDITAKTGD
                     PQLLVGASGAISACMGAFLIHFRKTEITFWYLLMYRSGTFRWPAWFALPLWLAEQFLY
                     SFLHSSMGVTSQVAYASHIGGFAVGMAGGYAMSKLFPHDADHDDVEYSEPMPPVRKQI
                     DAALDERISKCLAALKARDQATVRQLSSRVILDLARVDHDGRILDLYREIEKQFTTVP
                     LTDGAFAAAATAADRLNDRRTFVAIAGSMMEAHPGSTQLPKVMWRLAQIHREAGEYEL
                     EQETLRTLAQRFTKDPLGQKAQLELDRQA"
     gene            4274..6718
                     /locus_tag="VFV99_14140"
     CDS             4274..6718
                     /locus_tag="VFV99_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5060502.1"
                     /translation="MTTYLRAGLVGMLVAAGCGDNIKADPDELGLLKVSKLGGLETTE
                     AGGTDSFEVELIGMPRENILVKFKSTNTDEGTVDVVEYEFERHTWDKPVVITVKGVDD
                     DYDDKDQLFEIDVDAGEFGHAHVPVANRDDDVFGAAVTATTGLQTSESGTTAQFTVKL
                     TSKPYGNVTIPLSSNRPTEGSLTTAMLTFTPLDWNAAQIVTVTGVDDSVADDNQNYVV
                     TLGSTTSSDARDAAIDPEDVSLINLDNDLANFLVTPTVGLQTTESQGTATFTVQLLSQ
                     PSANVTFDVASDTPSEGTASPATLTFTPLNWNAPQQVTITGANDDVADGNKPYTIVLN
                     NVVSSDAQYVLRDPSDVAVTNLDNDTAGVSVSLATTAETTESGGTADFDVVLLSQPLT
                     PVTFAVSSGNATEGTASVTSLTFTDTNWNAPQRVTVTGQNDNVADGDQHYAIVLDPDA
                     SYVDVNNQPVDPDDVSLINRDNDTAGVQLSRTTGLVTDETGLSDVFTVVLLSQPVDDV
                     TISYMSSNTAEAIVSPLSPITFTPANWDAPQTISAKGVDDVVRDGNQQFQILITTAST
                     DANYDNVPVPNVFGVNLDDDAAAIRVTPTELEVSEFGDSDTFDVVLVAQPTASVTIPI
                     TSSDTTEGTVDKSSLTFTTANWNVPQTVTVTGVNDALIDGQQQFFIRTGAVVSTDPFY
                     VGVDADDVTVLNFDDESPGVYVQAHNLLKTQEGTNQPQHIRMRLTLAPTAPVTCVVTV
                     SDPTEIALTPSPSVFTFDATNFSVLQTFDVRGVDDAIVDGDQLVTVITQPCTSADPVY
                     NLFDPRNIRVLNRDNE"
     gene            6885..>8959
                     /locus_tag="VFV99_14145"
     CDS             6885..>8959
                     /locus_tag="VFV99_14145"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5060503.1"
                     /translation="MTALVATSGTVPHEAEVVPPTFAIVESLARPVAPPHVVVAPAPS
                     ITARVIEDGEPVADAEVSISDGSKPLLATARTDRDGIVRFPELEPGAYELWAAHAEKA
                     SAIARVMDVTPDSRVDIVLDRPAAALSGHLVVDGELPGAATVQLVPMDVDHAVRVATV
                     DGHGEFAFSSLPYGRWRVEVSAPGHVQVEEQTIAIAAAPDELAVRLQRTGVVTGTVVD
                     PAGSPIAHATIVLRDQAGTTQRPISLTASRLRWVHPLAGSRVVPMNDMTGFGAPRAGS
                     RPAECGRGHCGIDLVQPRGSIVHAVADGQIAALFPDSRTEAGRVVVIHHGGGLKSFYM
                     HLDEVRPGLEVGQAIHAGDPVGTLGSTGFTHSVPHLHFAITHEDSGRAWYLDPEPMIR
                     AAVVLPAPRPYDPVDVAVAGAAKADLGAPVVATITTDAKGAFRVDGVAPGSYVAAAFA
                     SDLAPGVSAPFTVRSGDDIAGIVIQLTEGVLVQGRVTGRDGPIAGATVIATAGMGESA
                     HKIATTTTDKYGEYTLRSLGGKISLVASAASYGEAERAIAIIEGREHQREDFKLTIED
                     GQLRGVVLAPDGGAGAGISVRIVEGITRRRTVSDAFGRFTIGPVASGRYVVELSSLDF
                     PSKRMTIDSDRFAELRLEAGGGARALIRDVHSGSPLANIRIDATGPGGQTAFRTTDAR
                     GLVELRGLAA"
BASE COUNT         1567 a         2937 c         2952 g         1503 t
ORIGIN      
        1 gatcccgcga caggtctcgg cacccgctac acggaggcgt cgttcatcgc cacgatgcgc
       61 accggcaaga agcccaacgg cacgcccatc atgccgccca tgccctcgga cgtgtacaag
      121 aacatgaccg acgacgatct cggcgcaatc ttcgcctatc tgcagaccat cccggcgatc
      181 aagaaccagg tcccggatcc gctcgcgccc caacaagcgc agctcgtggc gaacccgcag
      241 cagatcgcga gtcagcaatg agtcgcgtcg atctgttcac tgtgatccac aaagggatcc
      301 gatcgctgtt gttcgagctc gcacgtgagg cggcgcgcgt cgatctgacc tcgacgcacg
      361 cggtcgatgg tttggtcgcg cgggtcgagc gcacactcgg gtttctcgac gagcacgcac
      421 ggctcgagga catgcacgtg ttcaccgcac tgcgcgctct cggtggtgag ctcgcagacg
      481 agctgaacgc cgaacatcgt agcctcgagg tcgtgcagct cgaggtcgag cgagcagccg
      541 atgctctcgc gctcgcgcag cttgctgggc gggaagaggc aggcgcgcac ctcgcgcgac
      601 tgatcaacca cctcgtcgcg gtacagctga tccacatgaa tcgcgaggag accgaggtca
      661 acgcgacact gtgggccggc ctgcctgaca gcgagctcgg ggcgatcaga acccggctga
      721 agaacacgct gtcgccggag cgcttcgacg agtggatgca gatcgtcgga cctgcgctca
      781 acccagtcga gcaccggctg gtaatcggtt aagattcgcg ggttgtccgc gtcctatcca
      841 ccgttaccgc ccgagctggt cacgtttgcc gaaggcggcg tatcgattct cgtcggtacg
      901 tgcaatcgcg agctcgtgcc cgattgcgtg cgcgggatgg gcgtgcgggt ctggccgggc
      961 gcaagcggtt tgacggtgct gctaccggcg gcgactgccg cggcatcgat cgcgaacgtg
     1021 cgcgacaacc cgcggctcgc ggtcacgctg tcgcacattc cgtcgcatcg tacgatccag
     1081 gtcaaaggca aggtgatcgc ggtgcgcgac ggcgacgacg cggatcgcga gctggcgacg
     1141 cgctatcgcg aaaagctcgc cgatgatctc gcgttcgtcg gccagccggc ggcgaacacg
     1201 ctgcggctcg gcatctggcc gtgccacgcc gtcgacctcg agatcgaggt cgtgttcgca
     1261 cagacgccgg gcccggtcgc cggtgtgaag atgccgctgc aaacggggcg gctgtgatcc
     1321 ggctcgacgc actcgatcag tgctttcacg gcatcgtgcc gggcgtgctc gcgacgtgcg
     1381 atcgggaagc gattccgaac gtcagctaca tcagccaggt gcacctgatc gacgagcagc
     1441 acctcgcgct gtcgtgccag ttcttcaaca agacgcggca gaacctcgac gagaatccgc
     1501 tcgcgacgct cgagctctac gatccgctga cgttcgaagc gtaccggttg cggctgcgct
     1561 tcttgcgctc ggagtcgacc ggcccgctgt tcgattcgat ggcggtgcgc atcctcgcga
     1621 tcgcgtcgca caccggcatg gcgggcgtat tcaagctgcg ctcggccgac gtgttcgagg
     1681 tgctgtcgat cgaacagcgc gagggctaca tcgagccgcc ggccgcgggt gaacgcctcg
     1741 tccgttcgct gccgaacggt ccgctcaccg agctccgcgg cttgcagctg gtgtcggagc
     1801 gggtcgcacg cgcgagcgat ctcgattcgc tgctgaccgg cgcactcgcc gcgctcgacg
     1861 aggtattcgg cttcacgcac ggcatgatca tgatgccgga gcacggcaag aagctcgtca
     1921 ccgtcgcgag tcacggctac ggcgacgcgg cgatcggtgc cgaggtgggg tgtggcgaag
     1981 gcatgatcgg caacgtcgcc gacacgaagc ggctcgtgcg catcgcatcg gcaagcgcgg
     2041 agctccgcta cggccgcgcg atccgcgagc agtacgcaca tacgggcagt ggtgtcggcg
     2101 ccgagattcc actgccgcgc ctcgccgacg ccgagagcca aatggcgatc ccgttgctcg
     2161 tacaggatcg gctcgtcggg gtgctcgcgc tcgagtgccg gtcgcgcctg aacttcgagc
     2221 agtggcacga agcgttcctc caggtgctcg cgaatcagat cgcaagcggc atcgagcgca
     2281 tggccgagcg ggaggacgac ggcgcgatgt cgctgcccgc cgctgctccg cccaagcggg
     2341 tgccggcagc gcgcacgctc aacttcacgt actaccgcaa cgacgattgc gtattcgtcg
     2401 acggcgagta cctgatccgc aacgtgccgg gcaagatcct gtggaaggtg ctgcgagcgt
     2461 acgaagcgga gaagcgcact gatttttcga accgcgagct gcgactcgat ccgacgctcg
     2521 gcttgccgcc gatcaaggac aacctggaga gtcgattgat cctgctgcgc aagcggctcg
     2581 agcagaagtg ccccgacgtg cggctcgtac cgacgcggcg cggccggttc tcactgtgca
     2641 tcggctgcga gctcgagctc gtcgagcgcg agaccgcctg agctgatcat tacttctcgc
     2701 ggtcgccgcg atcaccctga ccgcgtttcg ccgtcgactt cgcctttcgc ttctgatcgc
     2761 gggcgctcga acgaccgacg ttctcttctt ccttgaattg gccgcgttcg ttacggcgaa
     2821 cgtagcgctt gttcccacga ggactgatcc gagtgcgttt ggtagccata aggtattccc
     2881 tggcccctag cggtgcagtg acgatgccaa acgctcaggc ctgacggtcg agctcgagct
     2941 gcgccttctg gccgagtgga tccttggtga agcgctgcgc gagcgtgcgt agcgtttcct
     3001 gttcgagctc gtactcgccg gcttcgcggt ggatctgcgc gagccgccac atcaccttgg
     3061 gtaactgcgt actgccggga tgcgcttcca tcatcgaacc cgcgatcgcg acgaacgtgc
     3121 ggcgatcgtt gagccgatcg gcggcggttg cagcggcagc gaatgcgccg tcggtgagcg
     3181 ggacggtcgt aaactgtttc tcgatctctc ggtacagatc gagaatccgg ccatcgtgat
     3241 cgacgcgcgc gagatcgaga atgacgcgtg acgacagctg gcggacggtt gcctgatcgc
     3301 gcgccttcag ggcggcgagg cacttcgaga tgcgttcatc gagcgcggca tcgatctgct
     3361 tgcgcaccgg cggcatcggc tcgctgtatt cgacgtcgtc gtggtcggca tcgtgcggga
     3421 acaacttgct catcgcgtac ccaccggcca tgccgacggc gaaaccgccg atgtgcgacg
     3481 cgtacgcgac ctgggacgtc acgcccatgc tgctgtgcag gaacgagtag aggaactgct
     3541 cggcgagcca cagcggcaac gcaaaccacg cgggccaccg gaacgtgcca ctgcggtaca
     3601 tgagcaggta ccagaacgtg atctcggtct tgcggaagtg gatgagaaat gcgcccatgc
     3661 acgccgagat cgcacccgat gcgccgacga gcagctgcgg atcgccggtc ttcgccgtga
     3721 tgtcgtacgc gaaggccgag gcggcggcgc cgagcaaata gaacagcgtg aagcgcacgc
     3781 ggccgtagcg atcctcgagc gctgatccgg cgagccagag gaacagcatg ttgccgatca
     3841 ggtgcatcca gccgccgtgg acgaacgcgg aggtgatgag gccggtctgg agcccctcgt
     3901 gggtcttgta gccaaatcgg tacgccggaa gcttgttcgc gatcgcttcg acctgacggt
     3961 cgtgctcctg ctcctgttcg ggtgtcagct ggccgtcctc gttctcggcg gccatgtccg
     4021 tgtagcgatc cgtgagcgca attagctcgt ttgcgagctg gtcttcgcta ggcgcgccga
     4081 gcgtcgcgta cagctgcatc agcgtgcaca gcgcgatgat ggcgatcgtg acccatggca
     4141 ttcgccgaat ggactggtcg tgcccgatgg gaacgatcac gagaacatcg tagaccgttt
     4201 tgccgggtag gcgccggtta tcggcagggc cgggatactg cacgggcgcg cgaacttcga
     4261 taacctgggt ttgatgacta cgtaccttcg tgcggggcta gtgggcatgc tcgtggccgc
     4321 gggatgcggt gacaacatca aggccgaccc ggacgagctc gggctgctca aggtcagcaa
     4381 gctcggtggg ctcgagacga ccgaggcggg cggtaccgat tcgttcgagg tggagctgat
     4441 cgggatgccg cgcgagaaca ttctcgtgaa gttcaagtcg acgaataccg acgaaggcac
     4501 ggtcgatgtc gtcgagtacg agttcgagcg ccacacatgg gacaagcccg ttgtcatcac
     4561 cgtgaagggc gtcgacgacg actacgacga caaggatcag ttgttcgaga tcgatgtcga
     4621 cgcgggcgag tttggccatg cgcatgtccc cgtcgcgaac cgcgacgacg acgtcttcgg
     4681 cgccgctgtg acggcgacaa ccggcttgca gacgagcgag tccgggacga cggcgcagtt
     4741 caccgtcaag ctgacgagca agccgtacgg caacgtcacg atcccgctca gcagcaatcg
     4801 gccgaccgaa ggctcgctga cgacggcgat gctgacgttc acgccgctcg attggaacgc
     4861 ggcgcagatc gtgacggtga ccggtgtcga tgactcggtc gctgatgaca accagaacta
     4921 cgtcgtcacg ctcggctcga cgacgagcag cgacgctcgt gacgcggcga tcgatcccga
     4981 ggacgtctcg ttgatcaacc tcgacaacga cctcgcgaac ttcctggtca ccccgaccgt
     5041 cggtctgcag accaccgagt cgcaaggcac tgcgacgttc acggtgcagc tgttgtcgca
     5101 accgagcgcg aacgtgacgt tcgacgtcgc ctccgatacg ccgagcgagg gcacggcgtc
     5161 gccggcgaca ttgacgttca ctcccctgaa ctggaacgca ccgcagcagg tcacgatcac
     5221 gggcgctaac gacgacgtcg ccgacggcaa caagccgtac acgatcgtgc ttaacaacgt
     5281 cgtgtcgagt gatgcgcagt acgtgctgcg cgatccgtcc gacgtggcag tcaccaacct
     5341 cgacaacgac acggccggtg tcagcgtctc gctcgcgacg accgcggaga caaccgagag
     5401 cggtggcacg gcggacttcg acgtcgtctt gctatcgcag ccgctcacgc ctgtcacgtt
     5461 cgccgtctcg agcggcaatg cgaccgaggg caccgcgtcg gtaacatcgc tgacgtttac
     5521 cgatacgaac tggaacgcgc cgcaacgggt cacggtcacc ggccagaacg acaacgtcgc
     5581 cgatggcgat caacactacg cgatcgtcct cgacccagat gcgagctacg tcgacgtcaa
     5641 taaccaaccg gtcgatccgg acgacgtttc gctcatcaat cgcgacaacg atacggcggg
     5701 cgtgcagctg tcgcgtacga cgggtctcgt caccgatgaa accggtcttt cggacgtgtt
     5761 cacggtcgtg ctgctgtcgc agccggtcga cgacgtgacg atcagttaca tgtcctcgaa
     5821 tacggccgag gcgattgtct cgccgctgag cccgatcacg tttacccctg ccaactggga
     5881 cgcgccgcag acgatcagcg cgaagggcgt cgatgacgtc gtgcgtgacg gtaaccagca
     5941 gttccagatc ctgatcacca ccgcgagcac cgacgcgaat tacgacaacg tgccggtgcc
     6001 gaacgtgttt ggcgtcaacc tcgacgacga tgccgccgcg attcgcgtga cgccgaccga
     6061 gctcgaggtc tccgagttcg gtgacagcga cacgttcgat gtcgtgctcg tcgcccagcc
     6121 gaccgcgagc gtcacgattc cgatcacgag cagcgacacg accgagggaa cagtcgacaa
     6181 gagcagcctg acgttcacga ccgcgaactg gaacgtgccg cagaccgtca cggtcaccgg
     6241 cgtcaacgat gcgttgatcg atggccagca gcagttcttc atccgcactg gcgcggtcgt
     6301 atcgaccgat ccgttctacg tcggtgtcga tgccgacgac gtcacggtcc tgaacttcga
     6361 cgacgagagc ccaggcgtct acgtgcaggc ccacaatctg ctcaagactc aggaaggcac
     6421 caaccagccg cagcacatcc gcatgcgcct cacgctcgcg ccgaccgcgc cggtcacgtg
     6481 cgtcgtcacg gtgtcggatc cgaccgagat cgcgctcacg ccgtcgccga gcgtcttcac
     6541 gttcgacgcg acgaacttct cggtgctgca gacgttcgac gtccgcggtg tcgacgatgc
     6601 gatcgtcgat ggcgatcagc tggtcaccgt gatcacccag ccctgtacga gcgcggaccc
     6661 ggtctacaac ctgttcgacc cgcggaacat ccgcgtgctc aatcgagaca acgagtagcg
     6721 atttggcgtg actatttggc agccgggtat gcgtgccagc gcgcaagccc gcgagctgcc
     6781 acgggtcgta cgggcatgat ggctgcacta ctgcgtacgt gggctggcgt ctagctgaca
     6841 tacggagacg gtgtcgtcgt ggcgccgccc cgctgctctg ggcggtgacc gcactcgtcg
     6901 cgacgagcgg cacggtgccg catgaggccg aggtcgtgcc gcccacgttc gcgatcgtcg
     6961 agtcgctcgc gcggccggtc gcgccgccgc atgtcgtcgt cgcgcctgcg ccgagcatca
     7021 ccgcccgcgt catcgaagac ggtgagcccg tcgctgacgc agaggtttcg atcagcgatg
     7081 gcagcaagcc cttgctcgca actgcacgca cggatcgcga cggcatcgtc cgctttcccg
     7141 agctcgagcc cggcgcctac gagctgtggg cggcacatgc ggagaaggcg tcggcgatcg
     7201 cgcgcgtgat ggacgtgacg ccggactcgc gcgtcgacat cgttctcgat cgcccggctg
     7261 ccgcgctgag tggtcacctc gtcgtcgatg gcgagctgcc cggagcagca accgtgcagc
     7321 tcgtgccgat ggatgtcgat cacgcggtgc gcgtcgcgac tgtcgacgga cacggcgagt
     7381 ttgcgttttc gtcgctgccg tacggccggt ggcgcgtcga ggtcagcgcg ccggggcacg
     7441 ttcaggtcga ggagcaaacg atcgcgatcg ctgctgcgcc cgacgagcta gccgtgcggt
     7501 tgcaacgcac cggcgtcgtg accgggaccg tcgtcgatcc ggcgggctca ccgatcgcac
     7561 acgcgacgat cgtgttgcgc gatcaagccg ggacgacgca gcgcccgatc tcgctcaccg
     7621 catctcgcct ccgctgggta catccgctcg ccggtagtcg cgtcgtgccg atgaatgaca
     7681 tgaccgggtt tggcgcaccg cgcgcgggct cgcgcccagc cgagtgcggc cgtggccact
     7741 gcggtatcga cctcgtgcag ccgcgcggca gcatcgtcca cgcggtcgcc gatggtcaga
     7801 tcgcggcgct gttcccggac agccgcaccg aagcaggccg cgttgtcgtc atccatcacg
     7861 gcggcggcct caagtcgttc tacatgcacc tcgacgaggt gcgccccggc ctcgaagtcg
     7921 gccaggcgat ccacgccggc gatcccgtcg gcacgctcgg ctcgacggga ttcacgcatt
     7981 ccgtgccgca cctgcacttc gcgatcacgc acgaagacag cggccgcgcc tggtacctcg
     8041 atcccgagcc gatgatccgc gctgccgtcg tgcttcccgc gccgcgtcca tacgatcccg
     8101 tggatgtcgc agtggccggc gccgcgaaag ccgacctcgg tgcgccggtg gtcgcaacga
     8161 tcacgaccga cgcgaaaggc gcgttccgcg tcgacggcgt cgctcctggc tcgtacgtcg
     8221 cagcagcgtt tgccagcgat ctcgcgcccg gcgtcagcgc gccgttcact gttcgcagcg
     8281 gcgacgacat cgcaggcatc gtcatccagc tgaccgaagg cgtgctcgtg cagggcaggg
     8341 tgaccggccg cgatggtccg atcgcaggcg cgaccgtgat cgccaccgcc ggtatgggcg
     8401 aaagcgcgca caagatcgcg acgacgacga ccgacaagta cggcgagtac acgctgcgct
     8461 cgctcggcgg caagatctca ctcgtcgcga gcgccgcgag ctacggcgag gccgagcgtg
     8521 ccatcgcgat catcgaggga cgcgagcacc agcgcgagga cttcaagctc accatcgagg
     8581 atggccagct gcgcggcgtc gtgctcgcac ccgacggcgg agcgggtgca ggcatcagcg
     8641 tgcgcatcgt cgaaggcatc acgcggcggc gtacggtcag cgatgcattc ggccgcttca
     8701 cgatcggtcc ggtcgcgagc gggcgctacg tggtcgagct gtcgtcgctc gattttccgt
     8761 cgaagcgcat gacgatcgac agcgatcggt ttgcggagct gcgtctcgag gcgggtggcg
     8821 gcgcacgcgc gctgatccgc gatgtccaca gcggctcgcc gctggcgaac atacgcatcg
     8881 atgccaccgg cccgggcggc cagaccgcgt tccgcacgac cgacgcacgc ggcctcgtcg
     8941 agctccgcgg cctcgcagc
//
LOCUS       DASUFX010000355         7983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_474359,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000355 DASUFX010000000
VERSION     DASUFX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474359"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /gene="pyrF"
                     /locus_tag="VFV99_14150"
     CDS             complement(<1..494)
                     /gene="pyrF"
                     /locus_tag="VFV99_14150"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5060504.1"
                     /translation="MAFSVGNRLIAALDAPSRGEADALVTRLGGVPSWVKIGLELFCA
                     EGPSIVREYTKRGLSVMLDLKLHDIPETVARATAKVASLGAGLLTVHAGGGRAMLEAA
                     VKNAGDMRVLAVTVLTSLDDSDLADVGAQGPVAELVKRRAQLAHRAGCHGIVASPHEV
                     ASVRA"
     gene            complement(556..2412)
                     /locus_tag="VFV99_14155"
     CDS             complement(556..2412)
                     /locus_tag="VFV99_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX5060505.1"
                     /translation="MKLALITLILVLAGCPRHTRKTLTPDVPQHGDATARNRFLEAKS
                     RFLRDGGQSLEFKKIIQEYPADPIVPWAELYAGIASVKARSFAEADAQLTSLIERNVD
                     PGLTARAQLFLGITKNYEGDAATARKLLATSERAIENDDERTEYLAAVAYSTAVGDKP
                     LAALPVFDLLWQRVTPTEKALIVARCQELVSALDRNTLERLFDEIADRRGPAIGAVGS
                     RLVVIYDRAADTQRADKMRENMVPVRQALGLPRTITEAEVGSAQSGGGDAGLIGAVVP
                     LGSKKENRVAEQAVAGLGLAAGAPDGKGVVAIETRAAIDKTTSAEAVDALAQKNVIAI
                     IGPIGKDSVDGASARAENLGVPMISLSTSAEQRPTGRFIFHIRHSPEHRARTLAQRAL
                     AKGIKTFAIMAPETPYGKGISAAFAEAVKQGGGTVVITVTYPDTAKSFTKEASSLKSG
                     WDGIFIADEATRLGLIVPTLAAAGYVAKAQPWPKKMKNGRPILLLSTAEGLDASYLAA
                     AGRHSEGALFAPGFFPDAADATQKPFIDRFVAAYSRQPEATEAYAFDAAQIAAAAGAG
                     GRAGLATALAKSQLQGVTGAIQFNPDHRRSDPGVVYTVVEETGGVFAVRVAK"
     gene            2448..3461
                     /locus_tag="VFV99_14160"
     CDS             2448..3461
                     /locus_tag="VFV99_14160"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5060506.1"
                     /translation="MARQDRLVADVDRGFTALENGEIEEAEACLERCSKIDRKHADVV
                     ALAAAVADAQGDSETALGKYTELAALRPDDPMPRICLARLQLHALGDPDAALDTLDQA
                     FDFIDEEEDLVEAIVVRAEALIVTDDPEGARGALSELSTSAIEDPVLAFELAELSLAA
                     EDPRHALRWIEIAKKDKSATTDVQHLLGRVHEALGDHDETVKAWQAVRAADLAAPPGP
                     VAISDDELERIASAALEELPETARAKLEHVPILIDDVPSEELVADGFDPRTLGVIEGP
                     TMADAGDIATSPTHIRLFKKNLERIAHDLDDLAEEVRITVLHETAHYFGLEDDDLEKI
                     GLD"
     gene            complement(3458..3781)
                     /locus_tag="VFV99_14165"
     CDS             complement(3458..3781)
                     /locus_tag="VFV99_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060507.1"
                     /translation="MYAVFVVACATGGMNGGSGGDDVGEKMDAAVMPHYDSNVSSLPD
                     ASVKMDASIVVQDAFVPQPDAASGPFCMANSDCTNAGECCFSINGTGLCVPGTIVLGV
                     CFPIN"
     gene            4023..4730
                     /locus_tag="VFV99_14170"
     CDS             4023..4730
                     /locus_tag="VFV99_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060508.1"
                     /translation="MASLANQRTLLMRDLSGAATEYSPRRQAMLGVLGTAGAIAGYVG
                     VAVGTLLWWPTLPIVFGAGGAAGAYAALRKKPPVAPIDLHPVVTAKAAVTQRGIARRL
                     AETVTALTDEHVLAEQAVVRTRKGVLFRRIRAVPFLVELDGGDKLVVVGQVRIERSPA
                     LTTKIKTGDPRLAALDLGGIPIAAQLIVAVVNEGDPIEVTGEPATEIVPELAFHRDAG
                     EATVMRGRAQAVVAIRA"
     gene            complement(4731..>7983)
                     /gene="dnaE"
                     /locus_tag="VFV99_14175"
     CDS             complement(4731..>7983)
                     /gene="dnaE"
                     /locus_tag="VFV99_14175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5060509.1"
                     /translation="VLLAKNEVGYQNLSYLNSKGYLEGFYYNPRIDKQILKDRSEGLI
                     GMSACLGGEVAQTLEKHGVAQAEEVAREYQSIFGEGDFYLELMPTPIPEQQTLNDELK
                     RMGPKLGIPLVVTNDCHYVNRGDAAAHDVLMAIQTGKSLKDDKRLKHSVDSYYLKSPA
                     EVNAELRDCPEALENTVRIAKQCDVKLKLDQTHLPTYKVPPGEDLDSYIVKLVDQGLE
                     RRFGELAARGKKFDVDEYRERCKRELGVIQRMGFSGYFLIVWDFINWAKDHGIPVGPG
                     RGSGAGSAVAWALRITDLDPIEFKLLFERFLNPERVSMPDFDVDFCMNRRGEVIKYVE
                     GKYGSDRVGQIATFHQLKARGVIRDIARAMEIPFAEADKLAKLVPEPVQGKSPPVREA
                     IEQTPELKQLYNESPLHRELLDLAASLEGLNRNAGMHAAGVVIAEQPLWEYVPCFRGQ
                     NDEIVTQFAMKEVEKAGLVKFDFLGLKTLTVIQTAVKLINQQRPPGDELDIERIRKDD
                     ADVFKMIARGDTTGVFQLESSGFREMLKKLKPDCLEDIVAAGALYRPGPLEGGMVDQY
                     IDVKHGRKAADYMHPWLETVLKDTYGVIVYQEQVMQIAQVLGGYTLGGADLLRRAMGK
                     KKPEEMAKQKQTFLDGAKNKGVDVKRAAEIFELVEKFAGYGFNRSHSAAYGWVSYQTA
                     YLKHHYPHEFMAGLMSCDADNVDNIVKFIAEARSMGLTVERPDINESAQDFTVTSGEN
                     KVIRFGLGAVKGVGATAVEAILEARASEGKFESLFELCRRVDTQKCNRRVLEQLIKSG
                     AFDGLPGGHHRAQLLAALDAALERGAAEQRDRRSGQTSLFGMFAAAEPIKAPNGAAPA
                     GETYPEIEVWSHKQLLAFEKEALGFYVSGHPLDRYRGDLTRYATATTSDFAMGARSAG
                     EHSIGGIVSQYREMITKKGDKMARFMLEDAAGTLEVIAFPKTFEKVRHVLVSEDPILC
                     SGQVKNEGTSESPEWKMILEGAAPLSQLREQKTTRVDIHLNADMLTHDQIDELKTILA
                     NAQRGGCQAVVRLKISKRSETVIALPDAWAVSPTEDLLTRLERLFGDRVATLA"
BASE COUNT         1233 a         2736 c         2614 g         1400 t
ORIGIN      
        1 gcccggaccg aggcgacctc gtgcggcgac gccacgattc cgtggcaacc ggcgcggtgc
       61 gcgagctgcg cgcgacgctt caccagctcg gcgaccggtc cctgcgcgcc gacgtcggcg
      121 aggtcgctat cgtcgagcga agtcagcaca gtcaccgcga gcacgcgcat gtcgccggca
      181 ttcttgaccg cggcttcgag catcgcgcgc ccgccgccag cgtggaccgt gagcaggccg
      241 gcgccgagcg aggcgacctt cgcagtcgcg cgcgcgacgg tctcggggat gtcgtgcagc
      301 ttgaggtcga gcatcaccga caggccgcgc ttcgtgtact cgcggacgat cgatgggcct
      361 tcggcgcaga acagctcgag gccgatcttc acccacgacg gcacgccgcc gagccgcgtg
      421 acgagcgcat ccgcttcgcc gcgagaggga gcgtcgagtg ccgcgatgag tcggttaccg
      481 acggagaacg ccacgccggc tgtgtatcac acacccgggg gcgctgcccc gggttccccc
      541 cgggatgagc gacagttact tcgcgacgcg gaccgcgaag actccgccgg tttcctcgac
      601 gacggtgtag acgacgccgg gatcgctgcg ccgatgatcg ggattgaact gaatcgcgcc
      661 ggtcacaccc tgcagctgac tcttggcgag cgcggtcgcg aggccggcgc ggccgccggc
      721 acctgcggca gcggcgatct gtgcggcgtc gaatgcgtag gcctcggtgg cctcgggctg
      781 ccggctgtag gcggcgacga agcggtcgat gaacggcttc tgcgtcgcgt cggccgcgtc
      841 ggggaagaag cccggcgcga acagcgcgcc ttcggagtgc cggccggcgg ccgcgagata
      901 gctcgcgtcg aggccctcgg ccgtcgagag cagcaggatc gggcggccgt tcttcatctt
      961 cttcggccac ggctgcgcct tcgcgacgta accggctgcc gcgagcgtcg gcacgatcag
     1021 gccgaggcgc gtggcttcgt cggcgatgaa gatgccgtcc cagccgctct tgagcgacga
     1081 cgcttccttc gtgaaggact tcgcggtgtc gggataggtc acggtgatca cgaccgtgcc
     1141 gccgccctgc ttcactgcct cggcgaacgc cgccgagatg cctttgccgt acggcgtctc
     1201 gggcgccatg atcgcgaacg tcttgatgcc cttcgccagt gcgcgctgcg cgagagtgcg
     1261 cgcgcggtgt tcgggcgagt ggcggatgtg gaagatgaag cggcccgtcg ggcgctgctc
     1321 ggcactggtc gacagcgaga tcatcgggac gccgaggttc tcggcgcgtg cactggcgcc
     1381 gtcgacggag tccttgccga tcgggccgat gatcgcgatg acgttttttt gtgcgagcgc
     1441 gtcgacggct tcggcagacg tcgtcttgtc gatcgcggca cgcgtctcga tcgcgacgac
     1501 gcccttgccg tcaggcgccc cggctgcgag cccgaggccc gccaccgcct gctcggcgac
     1561 gcgattctct tttttgctgc cgagcggcac gaccgcgccg atcagacccg cgtcaccgcc
     1621 gccgctctgt gcagagccga cctcggcttc ggtgatcgtg cgcggcaggc cgagcgcttg
     1681 ccgcaccggc accatgttct ctcgcatctt gtcggcacgc tgcgtgtcgg ccgcgcggtc
     1741 gtagatcacg acgagccggc tgccgaccgc accgattgcg gggccacggc gatccgcgat
     1801 ctcgtcgaac aggcgctcga gcgtgttgcg gtcgagcgcg ctgacgagct cctgacaccg
     1861 cgcgacgatc aatgccttct cggtcggcgt cacgcgctgc cacaggaggt cgaacaccgg
     1921 caatgccgcg agcggcttgt cgcccacggc ggtcgagtag gcaaccgcgg caaggtactc
     1981 ggtgcgctcg tcgtcgtttt cgatcgcgcg ctcggatgtc gcgagcagct tgcgcgccgt
     2041 cgcggcgtcg ccctcgtagt tcttcgtgat gccgaggaac agctgtgcgc gcgcggtgag
     2101 gcccgggtcg acgttgcgtt cgatcaggct cgtgagctgc gcatccgcct cggcgaagct
     2161 gcgggccttg accgatgcga tgccggcgta cagctcggcc cagggcacga tcgggtccgc
     2221 ggggtactcc tggatgatct tcttgaactc gagcgactga ccgccgtcgc gcaagaagcg
     2281 actcttcgct tcgaggaacc ggttacgagc cgtggcatcc ccgtgctggg gcacgtcggg
     2341 cgtcaatgtc ttacgcgtgt ggcgagggca gcctgccagc accagaatca gcgtgatcag
     2401 cgcgagcttc acgtggagac gatagcaaat cgaggtaagc aacacccatg gcgcgccaag
     2461 atcgtctagt tgccgatgtc gaccgcggat tcaccgcgct cgaaaacggc gaaatcgagg
     2521 aagcagaagc ctgtctcgaa cgttgttcca agatcgaccg caagcacgcc gacgtcgtgg
     2581 cgctcgccgc agcggtcgca gatgcgcagg gcgacagcga aaccgcgctc ggcaagtaca
     2641 ccgagctggc agcgctccgt cccgacgatc cgatgccgcg aatctgcctc gcccgcctgc
     2701 agctgcacgc gctcggcgat cccgacgcgg cactcgacac gctcgaccaa gcgttcgact
     2761 tcatcgacga ggaagaagat ctcgtcgagg cgatcgtcgt gcgtgccgaa gccctgatcg
     2821 tcaccgacga cccagaaggc gcacgcggtg cgctgtccga gctgtcgacc tcggcgatcg
     2881 aggatccggt gctcgcgttc gagctcgccg agctatcgct cgccgccgag gatccgcgcc
     2941 acgcgctgcg ctggatcgag atcgcgaaga aggacaagtc cgcgacgacc gacgtccagc
     3001 acttgctcgg ccgcgtccac gaagcgctcg gcgatcacga cgagacggtc aaggcctggc
     3061 aggccgtgcg cgccgccgac ctcgccgccc cgcccggccc ggtcgcgatc tccgacgacg
     3121 agctcgagcg tatcgcgagc gccgcgctcg aagagttgcc cgagaccgct cgcgcgaaac
     3181 tcgagcacgt gccgatcttg atcgacgacg tgccatccga agagctcgtc gccgacggtt
     3241 tcgatccgcg cacgctcggc gtcatcgaag gccccacgat ggccgacgcc ggcgatatcg
     3301 cgacgtcacc gacgcacatc cggctgttca agaagaacct cgagcgcatc gcgcacgacc
     3361 tcgacgatct cgccgaggaa gttcgcatca cggtgctcca cgagaccgcg cattacttcg
     3421 gcctcgaaga cgacgacctc gagaagatcg gcctcgatta gttaatcggg aagcacaccc
     3481 cgagcacgat cgtgcccggc acgcacaggc cagtcccgtt gatcgagaag cagcactcgc
     3541 ccgcgttcgt gcagtcactg ttcgccatgc agaacggccc gctcgcggca tcgggttgcg
     3601 gcacgaacgc atcctggacg acgatgctcg catccatctt cacgctcgcg tcaggcagcg
     3661 acgatacgtt cgagtcgtaa tgcggcatca cggcagcgtc catcttctcg ccgacgtcat
     3721 cgccgccact gccgccgttc attccgcccg tcgcgcatgc aacgacgaag accgcataca
     3781 cgagcgtcca tcgtgtcgga agtgacatcg ttacccctcg gcgggcatcc tagctgcgag
     3841 cctccgtcgc tcaacgactt tcttgcgacg cgctgtcgat tgcgcaactc gcgcacctgc
     3901 tcgggtcagt tgtccccagc tatcctgcat tcgtgagcgt gagcgacgaa acattttgct
     3961 gcatgcattg ccggcttctg cagcctcggg gcaatgagtg cgtcgagtgt gcgtcgcctc
     4021 tcatggcttc gctcgcgaac caacgcacgc tgctgatgcg cgatctcagc ggcgctgcca
     4081 ccgaatattc gccgcgccgg caagcgatgc tcggcgtcct cggcaccgcg ggcgcgatcg
     4141 ccggctacgt cggcgttgcc gtcggcacgc tgctgtggtg gccgacgctg ccgatcgtct
     4201 ttggagccgg tggagcggcc ggcgcgtacg cggcgctgcg caagaagcct cctgtagcgc
     4261 cgatcgatct ccacccggtg gtcaccgcga aggccgccgt gacgcagcga ggcattgcgc
     4321 gccggctcgc cgaaacggtg acggcactga cggacgagca cgtgctcgcc gaacaagccg
     4381 tcgtgcgcac gaggaagggc gtcctgtttc ggcgcattcg ggccgtgccg ttcctggtcg
     4441 agctcgacgg tggcgacaag ctcgtcgtgg tcggccaggt gcgcatcgag cgatcgccgg
     4501 cgctgaccac gaagatcaag accggcgatc cgcgactcgc cgcgctcgat ctcggcggca
     4561 taccgatcgc ggcgcagctg atcgtcgcgg tcgtcaacga aggcgatcca atcgaggtca
     4621 ccggcgagcc tgcgaccgag atcgtgcccg agctcgcgtt ccatcgcgac gccggcgaag
     4681 cgaccgtgat gcgcgggcgc gcgcaggcag tcgtcgcgat ccgtgcgtga ttacgcgagc
     4741 gtcgcgacgc gatcgccaaa cagccgttcg agccgcgtca gcagatcctc ggtcggcgac
     4801 accgcccacg catcggggag tgcgatcacc gtctcggagc gcttcgagat cttgagccgc
     4861 acgaccgcct gacacccgcc gcgttgcgcg ttcgcgagga tcgtcttgag ctcgtcgatc
     4921 tgatcgtgcg tgagcatgtc cgcgttcaag tggatgtcga cacgcgtcgt cttctgctcg
     4981 cgcagttgcg acagcggcgc ggcgccttcg aggatcatct tccactcggg cgactcgctc
     5041 gtgccctcgt tcttgacctg gccactgcac aggatcggat cctcgctgac cagcacgtgg
     5101 cgcaccttct cgaacgtctt cgggaacgcg atcacctcga gcgtgccagc ggcatcctcg
     5161 agcatgaagc gcgccatctt gtcgcccttc ttcgtgatca tctcgcggta ctggctgacg
     5221 atgccgccga tcgagtgctc accggcgctg cgtgcgccca ttgcgaagtc gctcgtcgtc
     5281 gcggtcgcgt agcgcgtgag gtcgccgcgg tagcgatcga gcgggtggcc cgacacgtag
     5341 aagccaagcg cttccttctc gaacgcgagc agctgcttgt ggctccacac ttcgatctcg
     5401 ggataggtct cgcccgccgg cgccgcgcca ttcggcgcct tgatcggctc cgctgctgcg
     5461 aacattccga acagcgacgt ctgaccgctg cgtcgatcgc gctgctccgc ggcgccgcgc
     5521 tcgagcgctg catcgagggc cgccagcagc tgcgcacggt gatgcccgcc cggcaggccg
     5581 tcgaacgcgc cgctcttgat cagctgctcg agcacgcggc gattgcactt ctgcgtgtcg
     5641 acgcgccggc acagctcgaa tagcgactcg aacttgccct cgctggcgcg cgcttcgagg
     5701 atcgcctcga ccgcggtcgc accgacgccc ttcaccgcgc cgagcccgaa ccggatcacc
     5761 ttgttctcgc ccgacgtcac cgtgaagtcc tgcgccgact cgttgatgtc gggacgttcc
     5821 accgtcagcc ccatcgaccg cgcctcggcg atgaacttga cgatgttgtc gacattgtcg
     5881 gcgtcgcacg acatcaaccc cgccatgaac tcgtgcgggt aatggtgctt gaggtacgcc
     5941 gtctggtagc tgacccagcc atacgcggcc gagtgcgaac ggttgaagcc gtacccggcg
     6001 aacttctcga cgagctcgaa gatttcagcc gcgcgtttga cgtcgacgcc cttgttcttc
     6061 gcgccgtcga ggaatgtctg cttctgcttc gccatctcct cgggcttctt cttgcccatg
     6121 gcgcggcgaa gcagatcggc gccgccgagc gtgtagccgc ccagcacctg cgcgatttgc
     6181 atcacctgct cctgatagac gatgacgccg tacgtgtcct tgagcaccgt ctcgagccac
     6241 gggtgcatgt agtccgcggc cttccggccg tgcttgacgt cgatgtactg atcgaccatg
     6301 ccgccttcga gcggacccgg gcgatacaac gcgccggccg cgacgatgtc ctcgaggcag
     6361 tcgggcttga gcttcttcag catctcgcgg aagccgctcg attcgagctg gaacacgccg
     6421 gtcgtgtcgc cgcgcgcgat catcttgaac acgtcggcgt cgtccttgcg gatgcgctcg
     6481 atgtcgagct cgtcgccggg cggtcgctgc tgattgatca gcttcaccgc ggtctggatc
     6541 acggtaagcg tcttgaggcc gaggaagtcg aacttcacca agcccgcctt ctcgacctcc
     6601 ttcatcgcga actgggtgac gatttcgtcg ttctgaccgc ggaagcacgg cacgtactcc
     6661 cacagcggtt gctcggcgat cacgacgccg gctgcgtgca tgccggcgtt gcggttcaag
     6721 ccctcgagcg acgcggccag atcgagtagc tcgcggtgca gcgggctctc gttgtagagt
     6781 tgcttgagct cgggcgtctg ctcgatcgct tcgcgcaccg gcggcgactt gccttgcacc
     6841 ggctcgggca cgagcttcgc gagcttgtcg gcctcggcga acggaatctc catcgcacgc
     6901 gcgatatcgc gaatcacgcc gcgcgccttg agctggtgga acgtcgcgat ctggccgacg
     6961 cgatcgctgc cgtacttgcc ctcgacgtac ttgatcacct cgccgcgccg gttcatgcag
     7021 aagtcgacgt cgaagtcggg catcgacacg cgctcggggt tcaagaaccg ctcgaacagg
     7081 agcttgaact cgatcggatc gaggtccgtg atccgcagcg cccacgcgac cgccgagccc
     7141 gcgcccgagc cgcggcccgg cccgacgggg atgccgtggt ccttcgccca gttgatgaag
     7201 tcccagacga tcaggaagta gcccgagaac cccatccgct ggatcacgcc gagctcgcgc
     7261 ttgcagcgct cgcggtactc gtcgacgtcg aacttcttgc cgcgcgccgc gagctcgccg
     7321 aaccggcgct cgagcccctg atcgacgagc ttgacgatgt acgagtcgag gtcctccccg
     7381 ggcggcacct tgtacgtcgg caggtgcgtc tggtcgagct tgagcttgac gtcgcactgc
     7441 ttcgcgatgc gcaccgtgtt ctcgagcgcc tcggggcagt cgcgcagctc ggcgttcacc
     7501 tccgccggcg acttcaggta gtagctgtcg acggagtgct tgagccgctt gtcgtccttg
     7561 aggctcttgc cggtctggat cgccatcagg acgtcgtgcg ccgcggcgtc gccgcggttg
     7621 acgtagtggc agtcgttcgt cacgacgagc gggatcccga gcttgggccc catccgcttg
     7681 agctcgtcgt tgagcgtctg ctgctcgggg atcggcgtcg gcatcagctc gaggtagaag
     7741 tcgccctcgc cgaagatgct ctggtactcg cgcgcgacct cctcggcctg cgcgacgccg
     7801 tgcttctcga gcgtctgcgc gacctcgccg ccgaggcacg cgctcatccc gatcaggccc
     7861 tcgctgcggt ccttgaggat ctgcttgtcg atgcgcgggt tgtagtagaa gccctcgagg
     7921 tagcccttcg agttcaggta cgagaggttc tggtagccga cctcgttctt cgcgagcagc
     7981 acg
//
LOCUS       DASUFX010000356         4445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1862115, whole genome shotgun sequence.
ACCESSION   DASUFX010000356 DASUFX010000000
VERSION     DASUFX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862115"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..2349
                     /gene="ppk1"
                     /locus_tag="VFV99_14180"
     CDS             85..2349
                     /gene="ppk1"
                     /locus_tag="VFV99_14180"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5060510.1"
                     /translation="MALERPSIAPDPTTSGVHRLELVTEEPRERPSLRNFDVPGRTPI
                     APGVGDIVPTDLNAPELYLNRELTYLNFCWRVLHEAEDDRLPLLERVKFLAIVSSNVD
                     EFFMKRIGGLKQQVGAQLHSVTQDGRTPAQQIADCVELITKLEARKVHILDKVIAELK
                     AVGVWIAPYKELDTQQQAWVREYYLRNIFPLVTPQAMDPAHPFPFVSNLSLNLLVSLR
                     YEHDGEPLLARVKVPTSGGVSRFVRVGTSRTFVDLADVMANNLDMLFPGMEIEACAKF
                     RVTRNAITERDEEEADDLLEMIEIEMRKRKFAPVVRLQVDKFMDTHLRGRIAAELGLD
                     EGQDVFEADGMLGQRDVMEIAQLDIPELRDTPHAPAQPVDFLSDGNIFHAIRDQKHLM
                     VHHPYESFQESVERFFREAADDPKVRAIKTTLYRTSRDSAVIKHLVRAAQNGKQVAVV
                     LELKARFDEEANIKWASRMERAGIHVTYGVVGLKTHTKVTLVVRHDYDGLRRYAHVAT
                     GNYHAGTARLYSDLGLFTCDEGIGRDLTELFNYLTTGYKPRRKYQKLLVAPKQLKAAL
                     LEKIEREIALVGQGERGHIQWKLNAIEDVDIVRALYRAAQRGVTIDLIVRDTCRLRPG
                     LDGVSSSIKVVSVIGRFLEHARIYYFHNGGRPEYYIGSADAMQRNLEKRVEVLCPIED
                     PRLQSELRFILDTQLSDQRGAWDMLPDGSYAQRSGLNAKHSQVQMIERSERRLKEATR
                     LRRRKVQAASRRRR"
     gene            complement(2346..3221)
                     /locus_tag="VFV99_14185"
     CDS             complement(2346..3221)
                     /locus_tag="VFV99_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060511.1"
                     /translation="MGQAALVALLALAACNQVYGLEATAVLDAPYRVDGPPDAPQRCP
                     PLGTPLRATGVLNQVVRQNCFTYTASAGSQIAVATCEVLDEMGNKYVASEGRLDDSMA
                     PIDLAAPIGTTIDQAHISPEGDEIYVRATTAMVGAVVVYRRQADGKWTFGEQLPIAQT
                     ASFNIGGVTRRPHRHLLLLAGSAFYEYVSDDAGNWPETKQYTFAQLDVTLAQTPTISS
                     DGLRILFSGYSSIVGAMFQTRYAERAGIDDNFMGSQPVMNMPSAPDPFLIDDCSRIYF
                     SSASLQSVFAADLAY"
     gene            complement(3231..4175)
                     /locus_tag="VFV99_14190"
     CDS             complement(3231..4175)
                     /locus_tag="VFV99_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060512.1"
                     /translation="MNERVESEGFYEMLWDCDHCETKGLLGKSQRHCPECGAPQNPDK
                     RYFPSPEQQKKIDGHSYEGADRHCPACNAPMAAKAKNCAQCGSPLDGSKEVRGVVTPK
                     PAAKKRRRIWPYVVAALILIIFLIYWFFIRTKDAQLTVSAHRWERVVAIEKFGEYEES
                     AWRDQIPSDATGFPMCSRRQRSSRQVQTGEECHTERVDKKDGTFEQVKRCKPTYRSEP
                     IDDDWCTFRVRRWKKIDAVKAAGTGMTAAWPAQLPAADTPATLGATRSAGRTETLILD
                     FGGQSCDVKDATWQKYKDGQKVKVEVRASSGKLVCSSL"
     gene            complement(4229..>4445)
                     /locus_tag="VFV99_14195"
     CDS             complement(4229..>4445)
                     /locus_tag="VFV99_14195"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX5060513.1"
                     /translation="KYAPSPVAMGLAFVMSPSISITIAIGGFVHWFVARKSKKVADEQ
                     GVAWASALIGGEAIAGLLIAVLLLAER"
BASE COUNT          797 a         1453 c         1401 g          794 t
ORIGIN      
        1 cgctgttaac tgacgggagt ggctttgttg acgtaacggg ccgcttgtaa cggtcccgtc
       61 acctggcggg tataattttc gagaatggct ctcgagcgac cgagtatagc gcctgaccct
      121 acgacgtctg gcgtccaccg actcgagctg gtcaccgaag agccccgaga acggccgtcg
      181 ctgcgcaact tcgacgtgcc gggacgtacg ccgatcgctc cgggcgttgg cgacatcgtg
      241 ccgactgatc tcaacgcgcc cgagctctat ctcaaccgcg agctcacgta cctcaacttc
      301 tgttggcgcg tgctgcatga agcggaggac gatcgcctgc cgctgctcga gcgcgtgaag
      361 tttctcgcga tcgtcagctc gaacgtcgac gagttcttca tgaagcgcat cggcggcctc
      421 aagcagcagg tcggcgctca gttgcactcg gtcacgcagg acggccgcac gccagcacag
      481 cagatcgccg actgcgtcga gctgatcacc aagctcgaag cacgcaaggt ccatatcctc
      541 gacaaagtca tcgccgagct gaaggcagtc ggcgtgtgga tcgcgccgta caaagagctg
      601 gatacgcaac agcaggcgtg ggtgcgcgag tactacctgc gcaacatctt cccgctcgtc
      661 acgccgcagg cgatggatcc ggcgcacccg tttccgttcg tctcgaacct gtcgctcaac
      721 ctgctcgtgt cgctgcgcta cgagcacgac ggcgagccgc tgctcgcgcg cgtcaaggtg
      781 ccgacgagcg gtggcgtgtc gcgattcgtc cgcgtcggca catcacgtac gttcgtcgat
      841 ctcgccgacg tgatggcgaa caacctcgac atgctgttcc ccggcatgga gatcgaggcg
      901 tgcgcaaagt ttcgcgtcac gcgcaacgcg atcaccgaac gcgacgaaga agaagccgac
      961 gacctgctgg agatgatcga aatcgagatg cgcaagcgca agttcgcgcc ggtcgtacgt
     1021 ctgcaggtcg acaagttcat ggacacgcac ctgcgcggcc gcattgccgc ggagctcggc
     1081 ctcgacgagg gtcaggacgt gttcgaagcc gatggcatgc tcggccagcg cgacgtcatg
     1141 gagatcgctc agctcgacat ccccgagcta cgtgacacac cacacgcgcc cgcgcagccg
     1201 gtcgacttcc tctccgacgg caacatcttc cacgcgattc gcgaccagaa gcacctgatg
     1261 gtgcatcacc cgtacgaatc gtttcaggag agcgtcgagc gattcttccg cgaagccgcc
     1321 gacgatccga aggtgcgcgc gatcaagacg acgctgtatc gcacgtcgcg tgacagcgcc
     1381 gtgatcaagc atctcgtgcg tgccgcgcag aacggcaagc aggtcgcagt cgtcctcgag
     1441 ctcaaggcac gcttcgacga ggaagcgaac atcaagtggg cgtcgcggat ggagcgcgca
     1501 ggcatccacg tcacctatgg cgtcgtcggc ttgaagacgc acacgaaggt gacgctcgtc
     1561 gtgcgccacg attacgatgg cctgcgccgc tacgcgcacg tcgcgaccgg caactatcac
     1621 gctggcaccg cgcgtcttta cagcgacctc ggtctgttca cgtgcgacga gggtatcggc
     1681 cgcgatctga ccgagctgtt caactatctg acgaccggct acaagccgcg tcgcaaatat
     1741 cagaagctgc tcgtcgcacc gaagcagctg aaggcggcgt tgctcgagaa aatcgagcgc
     1801 gagatcgcgc tggtcgggca aggcgagcgc ggccacatcc agtggaagct caacgcgata
     1861 gaagatgtcg acatcgtgcg tgcgctgtat cgcgctgccc agcgcggcgt gacgatcgat
     1921 ctgatcgtgc gcgacacctg ccgcctgcgt cccggtctcg acggtgtgtc gagctcgatc
     1981 aaggttgtca gcgtgatcgg gcgcttcctc gagcacgcgc gcatctacta cttccacaac
     2041 ggcggccgcc ccgagtacta catcggctcg gccgacgcga tgcagcgcaa cctcgagaag
     2101 cgcgtcgagg tgctgtgccc gatcgaggat ccgcgcctgc agtcggagct gcgcttcatc
     2161 ctcgataccc agctctcgga tcagcgcggt gcgtgggaca tgttgcccga tggcagctac
     2221 gcgcaacgct cgggtctgaa cgcgaagcac agccaggtgc aaatgatcga gcgttccgaa
     2281 cgccgcctga aagaagcgac gcgcctgcgc cgccgcaaag tgcaagctgc ctcgcgccgc
     2341 cgccgttagt atgcgaggtc tgcagcgaac acggactgca gcgacgcgct gctgaagtag
     2401 atccgcgagc agtcgtcgat caggaatgga tcgggcgcgg acggcatgtt catcacgggc
     2461 tgcgagccca tgaagttgtc gtcgatcccc gcgcgctctg cataccgggt ctggaacatc
     2521 gcaccgacga tcgacgagta cccggagaac aggatgcgta gaccgtccga tgaaatcgtc
     2581 ggcgtttggg ccagcgtgac gtcgagctgc gcgaacgtgt actgtttcgt ctccggccag
     2641 ttgcctgcgt cgtcggacac atactcgtag aacgccgagc ccgcgaggag cagcaagtgg
     2701 cgatgcgggc gtcgcgtgac cccgccgatg ttgaagctgg cggtctgcgc gatcggcaac
     2761 tgctcgccga acgtccactt gccatcggct tgtcggcggt aaacgactac cgctcctacc
     2821 atcgccgtgg tggcgcgcac gtagatctcg tcgccctcgg gagagatgtg cgcttgatcg
     2881 atggtcgtgc cgattggtgc cgccaggtcg atgggagcca tcgagtcgtc gaggcggcct
     2941 tcgctcgcaa cgtacttgtt gcccatctcg tcgagaacct cgcaggtcgc gaccgcgatc
     3001 tgcgaacccg cgctggccgt gtacgtgaag caattctggc gcacgacctg gttgagcacg
     3061 ccggtcgctc gcaacggcgt gccgagcggc gggcaccgct gcggcgcatc aggcggaccg
     3121 tcgacgcgat acggtgcatc gagcacggcc gtcgcctcga gcccgtagac ctgattgcag
     3181 gcggcgagcg cgagcaatgc aacgagcgct gcctgcccca tccgatgatt ctacagcgac
     3241 gagcacacga gcttgccgga gctagcgcga acttcgacct ttaccttctg cccgtccttg
     3301 tatttctgcc aggtggcgtc cttgacgtcg cagctctgac caccgaagtc gaggatcagc
     3361 gtttcagtgc gacccgcaga gcgcgtcgcg ccaagcgttg cgggtgtatc ggcggctggc
     3421 aactgcgccg gccacgcggc ggtcatgccg gtgcccgctg ctttgaccgc gtcgatcttc
     3481 ttccagcgcc gcacgcggaa cgtgcaccag tcgtcgtcga tcggttcgga ccgatacgtc
     3541 ggcttgcagc gcttcacttg ctcgaacgtg ccgtccttct tgtcgacgcg ctcggtgtgg
     3601 cactcctcgc cggtctgcac ctgacgcgac gagcgctggc gccgcgagca catcggaaaa
     3661 cccgtcgcgt cgctcggtat ctgatcgcgc cacgctgact cttcgtactc gccgaacttc
     3721 tcgatcgcga cgacgcgctc ccagcgatgc gcgctgaccg tcagctgcgc atccttcgta
     3781 cgaatgaaga accagtagat gaggaagatg atcaggatca gcgcggcgac cacgtacggc
     3841 cagattcgcc gccgcttctt ggctgcgggc ttcggcgtga cgacgccgcg cacttccttg
     3901 ctcccatcga gcggcgagcc gcactgcgcg cagttcttcg ccttcgcggc catcggcgca
     3961 ttgcacgccg ggcagtgccg gtcggcgcct tcgtagctgt ggccgtcgat cttcttctgc
     4021 tgttcgggcg acgggaagta gcgcttgtcg gggttctgcg gcgcaccgca ctcgggacag
     4081 tggcgctggc ttttgccgag cagtcccttc gtctcgcagt ggtcgcagtc ccacagcatc
     4141 tcatagaagc cttcgctctc gacgcgttcg ttcacggagg aacgcattgt atgcgatcag
     4201 cgtcccgcga gcgcgcttgg ctcggccgct agcgttccgc tagcaacagc acggcgatca
     4261 agaggccggc gatcgcctcg ccgccgatca aggccgatgc ccacgccacg ccctgctcgt
     4321 cggcgacctt cttcgacttg cgtgcgacga accagtgcac gaatccgccg atcgcgatcg
     4381 tgatcgagat ggacggcgac atgacgaagg cgaggcccat cgccacgggt gagggcgcgt
     4441 acttc
//
LOCUS       DASUFX010000357         6310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1585939, whole genome shotgun sequence.
ACCESSION   DASUFX010000357 DASUFX010000000
VERSION     DASUFX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585939"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2068
                     /locus_tag="VFV99_14200"
     CDS             299..2068
                     /locus_tag="VFV99_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5060514.1"
                     /translation="MRARCWCAVLVLLIACDVGEVDPITLSNEAQARGQNPCDDGAIR
                     KKANIKPSGDGHRFVGTNGKDIIFGTAGDDVIFGNGGDDLICGLGGADIIFGGEGRDH
                     IFGGGGNDTIHGQGGSDRIWGGDGNDVLFGDILDDKLYGQDGDDTLIGGHGTDTLDGG
                     AGADFLRGDTGNDTFIGGGGYDVASFATALPPGQPEVKNDGSQNEITGVAIVSGRADG
                     DGGNEGLGDIESIVGSPFTDRIEAGGRNVQPTYGLDIVQNGNGPPPGPATPAGVVYVA
                     SATIGNELVDVGVHVLGTTGDDRYTVRGNGMVIVVEATAALAAGDGCANQGATTVRCD
                     VGAYVAARPHRRDPFHYIAAWGDNGADVIEFVGDFPRELEVHASGGRGSDHLIGGAEA
                     DVFFTGVTGNDRLEGRAGDDALLGESQHPLGWADGARPDTAFYDDGADVLDGGDGNDQ
                     LVVDYVCGGHKFIGGAGHDIAGFARSGKPAISAQLAGPARVHTQWYGFAANMDKCGNR
                     RDRWTEFVNKGTNADLEVLEASEGPDRLWGDDRNNVIWGREGNDEMYGLGGDDELLGA
                     NGNNHMDGGDGDDDVRDGDGVEE"
     gene            2108..2275
                     /locus_tag="VFV99_14205"
     CDS             2108..2275
                     /locus_tag="VFV99_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX5060515.1"
                     /translation="MLDWLVIAAYVVALLWALVEVVSSDRAAIYKLAWVAVMLVFPVF
                     GLVVYYATADR"
     gene            complement(2279..2836)
                     /locus_tag="VFV99_14210"
     CDS             complement(2279..2836)
                     /locus_tag="VFV99_14210"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5060516.1"
                     /translation="MSVGAYLQKLPDDRRTAIAKVRDVINANLPTGYEERLQWGMISW
                     CVSESVLPAKDVYNKQPLCLAALASQKSHMAVYLMSVYGDKNLRGWFEKAYKQTGKKL
                     DMGKSCVRFKSLDALPVELIGEAIAKVSVDKYVEVYRTVHGGKSAGAAKRAAGKAAAK
                     KSAAKKSAAKKPAKKSAAKKSARRN"
     gene            complement(2837..4702)
                     /locus_tag="VFV99_14215"
     CDS             complement(2837..4702)
                     /locus_tag="VFV99_14215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5060517.1"
                     /translation="MAKNLGGAIIDLLDSERVDLRAAAATVLAAVGKSDKSVQGALTG
                     RLADPDPSVRRIALDGLAEMSAGGIAPKLVPLLHGDDEILAERAASLLAQQGAEAEST
                     LKKEVRAGTVGARRVMAQLLIRRGTTGAIEAVLDQLSDHEFGEQALQLVRAELDTGNE
                     KLANLVEKSAIGRASESAKDLHKVWAKAQKTAAEAQKAAAKASKGKKKTNGANGTNGH
                     NGHNGHNGANSHSAADPLRDPEVALGIARLGSLLRLIGYLARPSTQSLLLKYADAEEP
                     RPIRLAAIAGLRRIVAQSEAKGTEKVIETLIDYADGDDYAVAQSAVDTLRGARIPESL
                     AKSFAGLAKSKNVMAQKLAMERLPAGGGASAVKALVEALGGDDPTARDAAARGLAKAP
                     ESVLPVTRALLATADEQVARRYSGVIRAHRGHVSNQAIDELVERVRELMDRHLKGKAS
                     ADQLVLERVLAELIADVAPAKHVELLFERAKRLRKAGKPVEAFGSLKPLLRSRADLDA
                     AIDDDQRFFLAQLSLEAAGEGVLRSMSADDPVFAQFARLAAKGYPVAKNLARNGDVTD
                     EQLYALGFRLIESGDGSNEELGAELLQGIIEERPRSKLAKAAKNKLKLTGHLDDD"
     gene            complement(4735..5361)
                     /locus_tag="VFV99_14220"
     CDS             complement(4735..5361)
                     /locus_tag="VFV99_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060518.1"
                     /translation="MRRIIVIAAIVLAGFGALSIRVVLEGRDALATGDDWILRGKPLE
                     AIQAFETSARWYLPLAPHVDEAYERLRKLGASEDPAVAIAAWRAIRGAARATRSLWTP
                     HADDLAAADAAIAKLAAQDPAGAPVEGDSPQAREAWQKTRLSRDVRPSIGAAVLAALG
                     IVLWIGGAVVLAWRGIDAAGAFARRPALAGGAAIIVGFFCWFLGLYNA"
     gene            5443..>6310
                     /locus_tag="VFV99_14225"
     CDS             5443..>6310
                     /locus_tag="VFV99_14225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060519.1"
                     /translation="MPGEVDDADAVALAATHASDEKGIAHDATVQSGDVGHEATLASG
                     ETVASGGAPLGPSTAARDLPEVDETRYTIGGEVGRGGLGRVLRARDEVLDRPVALKQL
                     FASDDNSRRRFIREAMITARLQHPAIIPVYDAGRLADRSPFYAMKLVGGKPLDEAMRG
                     TNTLAARLALMPTVLAVADAVAYAHSQRIIHRDLKPGNVLVGEFGETIVIDWGLAKDL
                     SIDDHEAVDAGPYRAVASTHTVAGAVLGTPGYMAPEQAAGQEVDERADVYALGAILYH
                     VVSGAIPHEGTTL"
BASE COUNT         1006 a         2087 c         2155 g         1062 t
ORIGIN      
        1 cgcgatcagg gctagcagcc gaacagcgtg atgacggccg tgatgttgta gcagtagtac
       61 cactggccgt tgcggcacca gctgtcgtgc accgcgcagc cgtagtggta gcagcagtcg
      121 ccgcacaccc acgaccagca cctacgagtt gtccaaaagg gaaggtgccg agcatcgcat
      181 cgcgtcagcc cacgttcgtt tgcggtgctc tcccaccatt cgtccgattt ccggaccgta
      241 attttgcgaa aggattacga gtcgcatcgg cacatcggtt gctgatgcag cgtgggcaat
      301 gcgagctcgc tgttggtgtg cggtactggt cctcttgatc gcgtgtgacg tcggcgaggt
      361 cgacccgatc acgctctcga acgaagcgca ggcgcgcggg cagaacccgt gcgacgacgg
      421 cgcgattcgc aagaaggcga acatcaagcc aagcggcgac ggtcatcgct tcgtcggcac
      481 gaacggcaag gacatcatct ttggcaccgc gggggacgac gtcatcttcg gcaatggcgg
      541 cgacgacttg atctgtgggc tcggcggggc cgacatcatc tttggcggcg agggacggga
      601 ccacatcttc ggcggcggcg ggaacgacac gatccacggc caaggcggga gcgatcggat
      661 ctggggcggc gacggcaacg acgtgctgtt cggcgacatc ctggacgaca agctctacgg
      721 gcaggatggc gatgacacgt tgatcggcgg gcatggcacc gacacgctcg acggtggcgc
      781 gggcgccgac ttcttgcgtg gcgataccgg caacgacacc ttcatcggcg gcggtggcta
      841 tgacgtcgcg agctttgcga ccgcgctgcc gcctgggcag ccggaagtga agaacgacgg
      901 ctcgcagaac gagatcaccg gcgtcgcgat cgtcagtggg cgtgccgatg gcgacggcgg
      961 taatgaaggg ctcggcgaca tcgaatcgat cgtcggcagc ccgttcaccg atcgcatcga
     1021 ggcaggtggc cgcaacgtgc agccgacgta cgggctagac atcgtccaga acggcaacgg
     1081 tccgccaccc gggcccgcga caccggcggg tgttgtctat gtcgcgagcg cgacgattgg
     1141 caacgagctc gtcgacgtcg gcgtccacgt gctgggcacg accggcgatg atcgctacac
     1201 cgtgcgcggc aacggcatgg tgattgtcgt cgaggcgact gccgcgcttg ctgcgggcga
     1261 cgggtgtgcg aaccagggcg cgacgacggt gcgttgcgac gtgggtgcgt acgtcgcggc
     1321 gcggccccat cgccgcgatc cgtttcacta catcgccgcg tggggcgata acggtgccga
     1381 cgtgatcgaa ttcgtcggcg actttccgcg cgagctcgag gttcacgcga gcggtgggcg
     1441 tggcagcgat catctgatcg gtggcgccga agcagatgtg ttcttcacgg gcgtcaccgg
     1501 caacgatcgt ctcgaaggcc gcgccggcga cgatgcgctg ctcggggaga gtcagcaccc
     1561 actgggctgg gccgatggcg ctcgtcccga caccgcgttc tacgacgacg gtgccgacgt
     1621 cctcgatggc ggcgacggca acgatcagct cgtcgtcgac tacgtatgtg gtggccacaa
     1681 gttcatcggt ggcgcgggtc acgacatcgc cggcttcgcg cgcagcggta agcccgcgat
     1741 cagcgcgcag ctcgccggcc cggcgcgcgt gcacacgcag tggtacgggt ttgccgcgaa
     1801 catggacaag tgcggcaatc gccgcgatcg gtggaccgag ttcgtcaaca agggcaccaa
     1861 cgccgatctc gaagtgctcg aggcaagcga aggtccggat cggctgtggg gcgacgatcg
     1921 caacaacgtg atctggggcc gcgagggcaa cgacgagatg tacggcctcg gtggggacga
     1981 cgagctgctc ggcgcgaacg gcaacaatca catggacggg ggcgacggcg acgacgacgt
     2041 tcgcgacgga gacggcgtcg aggaatagcg ctggccgtcg cgcggcgcct ggcgtatgac
     2101 acggcccgtg ttggactggc tggtcatcgc ggcgtacgtg gtcgcgctct tgtgggcgct
     2161 ggtcgaggtc gtcagctcgg atcgcgcggc gatctacaag ctcgcgtggg tcgccgtgat
     2221 gctggtgttc ccggtgttcg gcctcgtcgt gtattacgcg actgccgacc gctgacggct
     2281 agttccgcct tgcggacttc ttcgcagccg acttctttgc gggcttcttc gcggcggact
     2341 tcttcgcggc ggacttcttc gcggcggctt tgccagcagc gcgcttcgcg gcaccagcgc
     2401 tcttaccgcc gtggaccgtg cgatagacct cgacgtactt atcgacgctg accttcgcga
     2461 tggcctcgcc gatcagctcg accggcagcg cgtcgaggct cttgaaccgc acgcacgact
     2521 tccccatgtc gagtttcttg ccggtctgct tgtacgcctt ctcgaaccag ccgcgcaggt
     2581 tcttgtcgcc gtacacgctc atcaagtaga ccgccatgtg gctcttttgc gacgcgagcg
     2641 ccgcgaggca gagcggctgc ttgttgtaaa cgtccttcgc cggcagcacc gactcggaga
     2701 cgcaccacga gatcatgccc cactgcagcc gctcttcgta gccggtcggc aagttcgcat
     2761 tgatgacgtc acgaaccttg gcgatcgcgg tgcggcggtc gtcgggcagc ttctggagat
     2821 aggcgcctac gctcatttag tcgtcatcca ggtggccggt gagcttgagc ttgttcttcg
     2881 ccgccttcgc gagcttgctg cgcgggcgct cctcgatgat gccctggagt agctcagcgc
     2941 cgagctcctc gttcgagcca tcgccagact cgatgagcct gaagccgagc gcgtacagct
     3001 gctcgtcggt gacgtcgccg ttgcgcgcga ggttcttcgc gaccggataa cccttcgcgg
     3061 cgagtcgcgc gaactgcgcg aacaccggat cgtccgcact catcgagcgc agcacgcctt
     3121 caccggccgc ttcgagcgac agctgggcga gaaagaagcg ctggtcgtca tcgatcgcag
     3181 cgtcgagatc cgcgcgcgag cggagcaggg gcttgagcga tccaaacgcc tcgaccggct
     3241 tgccggcttt gcgcaggcgt ttcgcgcgct cgaacaacag ctcgacgtgc ttcgcaggcg
     3301 cgacatccgc gatcagctcg gcgagcacgc gctcgagcac gagctggtcg gccgatgcct
     3361 tgcccttcaa gtgacgatcc atcagctcgc gcacgcgctc gacgagctcg tcgatcgcct
     3421 ggttcgagac atggccgcga tgcgcgcgga tgacaccgct atagcggcgc gcgacttgct
     3481 cgtccgccgt cgcgagcagc gcgcgggtca ccggcaacac cgactccggc gccttcgcga
     3541 gaccgcgggc ggccgcgtcg cgtgccgtcg gatcatcgcc gccgagcgcc tcgacgagcg
     3601 ccttcaccgc cgatgcaccg ccgcccgcgg gcaagcgctc catcgcgagc ttctgcgcca
     3661 tcacgttctt cgacttcgcg agacccgcga acgacttcgc gagcgactcg ggaatgcgcg
     3721 cgccgcgcag cgtgtcgacg gcagattgcg cgaccgcgta gtcgtcgccg tcggcgtagt
     3781 cgatcagcgt ctcgatcacc ttttcggtgc ccttcgcttc gctctgcgca acgatgcggc
     3841 ggaggcccgc gatcgcggcg agccggatcg ggcgcggctc ttcggcgtcg gcgtacttga
     3901 gcagcagcga ctgcgtcgac ggacgtgcga gataaccgat caagcggagc agcgagccga
     3961 gccgcgcgat gccgagggcg acctcgggat cacggagcgg atccgcggcg ctgtgactgt
     4021 tcgcgccgtt gtggccgttg tggccgttgt ggccattcgt accgttcgcg ccgttcgtct
     4081 tcttcttgcc cttgctcgcc ttcgcggcgg ccttctgggc ttcggcggcg gtcttctggg
     4141 cctttgccca caccttgtgg aggtccttcg cggactcgct ggcgcggccg atcgcgcttt
     4201 tctcgacgag gtttgcgagt ttctcgttgc cggtgtcgag ctcggcgcgc accagctgca
     4261 gcgcctgctc gccgaactcg tgatccgaaa gctgatcgag caccgcttcg atcgcgcccg
     4321 tcgtgccgcg gcggatcaat agctgcgcca tcacccggcg cgcgccgacc gttcctgcgc
     4381 gcacttcttt cttcagcgtc gattccgctt ccgcaccctg ttgcgcgagc agcgacgcag
     4441 cgcgctcggc gagaatctcg tcgtcaccgt ggaggagcgg gacgagcttg ggcgcaatgc
     4501 cgcctgcgct catctcggca aggccgtcga gtgcgatgcg acgcacgctt ggatcggggt
     4561 ccgcgaggcg cccggtcagc gcgccctgta ccgacttgtc actctttccc accgctgcga
     4621 gcacggtcgc cgcggcagcc cgcagatcga cgcgctccga gtcgagcaga tcgatgatcg
     4681 ctccgcctag attcttggcc attccagcct ccggcgcgaa ggcaaccatg cgaatcaagc
     4741 gttgtaaagg ccgaggaacc agcagaagaa cccgacgatg atcgcagccc cgccggccaa
     4801 ggcggggcgt cgtgcgaagg cacccgcggc atcgatgcca cgccaggcca acacgaccgc
     4861 gccgccgatc cacagcacga ttccgagggc cgcaagcacg gctgctccga tgctcggccg
     4921 cacatcccgg gataaccgcg tcttttgcca ggcttcgcgc gcctgcggcg agtcgccctc
     4981 gaccggcgct cccgccggat cctgcgcggc cagcttcgcg atcgccgcat ctgccgccgc
     5041 gaggtcatcc gcgtgcggtg tccacagcga gcgcgtcgcc cgcgcggctc cgcgaatcgc
     5101 ccgccatgcc gcgatcgcga ctgcaggatc ttcggacgct cccagcttcc gcagccgctc
     5161 gtacgcctcg tcgacatgcg gcgcgagcgg cagataccag cgcgcgctgg tctcgaacgc
     5221 ctggatcgcc tcgagcggtt tgccgcgcaa gatccaatcg tctccggttg cgagcgcatc
     5281 gcgaccttcg agcaccacgc ggatcgagag cgcgccgaag ccggcgagca cgatggccgc
     5341 gatgacgatg atccgtcgca cgccggcact atacgtgagt agcgtcggcg cgcatgctgt
     5401 acgaggcgcc tacgtccggg ggctcgcgag tatgatggcg cgatgcccgg cgaagtcgat
     5461 gacgcggacg cggtagcgct cgccgccacg cacgcgtcgg acgagaaggg catcgcccac
     5521 gacgcgacgg tgcagagcgg cgacgtcgga cacgaagcaa cgctggcgag tggcgagacc
     5581 gtcgcgagtg gcggtgcgcc gctcgggccg agcacggctg cgcgcgatct gcccgaggtc
     5641 gacgagacgc gctacacgat cggcggcgag gtcggccgcg gtggtctcgg ccgcgtgctg
     5701 cgcgcacgcg acgaagtgct cgatcggcca gtcgcgctca agcaactgtt cgcgagcgac
     5761 gataactcgc ggcgccgttt cattcgcgaa gcgatgatca ccgcacggct ccagcatccg
     5821 gcgatcattc cggtctacga cgccgggcgg ctcgctgatc gctcaccgtt ctacgcgatg
     5881 aagctcgtcg gcgggaaacc gctggacgag gcgatgcgcg gtacgaacac gctcgcggcg
     5941 cggctcgcgc tgatgccgac ggtgctcgcg gtcgccgatg cagtcgcgta cgcgcacagc
     6001 cagcggatca ttcatcgcga cctcaagccg gggaacgtgc tcgtcggcga gtttggcgag
     6061 acgatcgtga tcgactgggg cctcgcgaag gatctgtcga tcgatgatca cgaggcggtg
     6121 gatgccgggc catatcgcgc ggtcgcgtcg acgcacacgg tggctggcgc ggtgctcgga
     6181 acaccgggat acatggcgcc cgagcaggcc gcagggcaag aggtcgacga gcgcgcagat
     6241 gtctatgcgc tcggcgcgat cctctaccac gtcgtgtccg gcgcgattcc gcacgagggc
     6301 acgacgctcg
//
LOCUS       DASUFX010000358         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1030219, whole genome shotgun sequence.
ACCESSION   DASUFX010000358 DASUFX010000000
VERSION     DASUFX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030219"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFV99_14230"
     CDS             complement(<1..1067)
                     /locus_tag="VFV99_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5060520.1"
                     /translation="MGELAPINKGFHGKALFVDLSARSHRVETIDPQVYRDYLGGYGL
                     GAWLMWKHFPAGTDPLDPKSMFALCSGMLTGLHAPFSGRIQIVGKSPLTNTWADSNSG
                     GSVCIHLRKAGFDALVVTGKADVPSIVVIRDGEVTIEPANELWGKEVPETFDALKDRF
                     GGKRDVGVSAIGPAGEKQMRIASVMNDRYHAFGRQGFGAVYGSKNLKAIVVAGTGEVP
                     VAKPDEFKAVCKRITDEYKRDLGLIARFFAWMLTAKVWLGWMYRAAKRMGAKLASPQA
                     AMRRSFSKRGTTLAVALSIENGDAPIKNWKGVGSRDFPIGKRGMKIDGGAVDKYITKK
                     LSCGDCPMPCKGIVKVKSRNLS"
     gene            1267..2319
                     /locus_tag="VFV99_14235"
     CDS             1267..2319
                     /locus_tag="VFV99_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060521.1"
                     /translation="MKTLASLTLALSLAVALTACGDNLGGGDDTGGEGSGSGSGSGSD
                     EPAPKLDATGTYRLNSTFDIATNMPGSSGSFVNGLIAATDDPDDPMSWLLDQMLDQMP
                     SGTLKSILQGAKPIVAGYLNDQLTQLAPDLVGTILELGHHMEDMTKHFGVNEKLVIAG
                     ADQTLLATLTADGVRFTVDANQIDVAFADYNVDDVVASGVLIQIENESKMLIGEHALP
                     LPYGKIVRMGLDTAIIPTIDPNAHNLADLLGDVVDCTSVGQGIADALDFGSASFWTSA
                     CTAGLNGAANLVYDQIAAKDSLLTFHLTGNARVSDGNGDHKLDKIQFGTWAGTLSYDA
                     TDTALVQPASFDGHRE"
     gene            complement(2799..3557)
                     /locus_tag="VFV99_14240"
     CDS             complement(2799..3557)
                     /locus_tag="VFV99_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060522.1"
                     /translation="MVAVWPRANYVVGGGPGEIALIALSTQKMPDPLPVSRKQHGMPD
                     EGSGANSVALVARDRAEDPAWFTESVIAPFADLIAADLGPQHAAAALAAEHAYVIEAQ
                     HDDPDDLGHLQAAWALAKCLCEEGATVVIDVYAARAHLGSEVAALSPERSFDIMHEVT
                     LFFDESDDGSIAAWSLGLRKFGRPDIVLLGLTPESATEAALLLRDIAATLASGERIEP
                     GDTVEGPDREFKAERFDPESAPVVAVEGDAILLR"
BASE COUNT          595 a         1222 c         1163 g          631 t
ORIGIN      
        1 gagaggtttc tcgacttcac cttgacgatg cccttgcacg gcatcgggca gtcgccgcac
       61 gacagcttct tggtgatgta cttgtcgacg gcgccaccat cgatcttcat gccgcgcttg
      121 ccgatcggga agtcgcgtga gccgacgcct ttccagttct tgatcggcgc gtcgccgttt
      181 tcgatcgaca gtgcgaccgc gagcgtcgtg ccgcgcttcg agaacgagcg gcgcatcgcg
      241 gcctgcggtg aggcgagctt cgcgcccatg cgctttgcag cgcgatacat ccagccgagc
      301 cacaccttcg ccgtgagcat ccacgcgaag aagcgcgcga taaggccgag gtcgcgcttg
      361 tactcgtcgg tgatgcgctt gcacaccgcc ttgaactcgt cgggcttggc gaccggcacc
      421 tcgccggtgc ctgcgacgac gatcgccttc aggttcttgc tgccgtacac ggcgccgaag
      481 ccctggcgac cgaacgcgtg atagcggtcg ttcatcaccg acgcgattcg catctgcttc
      541 tcgccggctg ggccgattgc cgacacgccg acgtcgcgct tgccaccgaa ccggtccttg
      601 agcgcgtcga atgtctcggg cacttctttg ccccacagct cgttcgcggg ctcgatcgtg
      661 acctcgccgt cgcggatcac gacgatcgac ggtacgtctg ctttgccggt gacgacgagc
      721 gcgtcgaagc ctgccttgcg caggtgaatg cagacgctgc cgccggagtt cgaatcggcc
      781 cacgtgttcg tcagcggtga tttgccgacg atctggatgc gaccggagaa cggtgcgtgc
      841 aggccggtca gcatgccgga gcacagcgcg aacatcgatt tcggatccag cggatcggtt
      901 cccgccggga agtgcttcca catcaaccac gcgccgaggc cgtagccgcc gaggtagtcg
      961 cgatacacct gcgggtcgat cgtctcgacg cgatgcgatc gcgcgctgag atcgacgaac
     1021 aacgccttcc cgtgaaaccc cttattgata ggagccagct cacccatagg ggccggagca
     1081 taccttcgat cgcgtgcggc ccccgccgac cggccgcgcc gtctagctgc gctggaaaaa
     1141 ccagccggga cgagatcttc taggcgcagc gaccaaatac ttggtgccaa caggctttct
     1201 tttgcctctt cgcgtcaaaa aaccctggca ttcgtggtcg aacctttgat agatgcccgc
     1261 gcctatatga agacgcttgc ttctcttact ctcgcactct cgctcgccgt cgccctcacc
     1321 gcgtgcggcg acaacctcgg cggcggtgac gacaccggcg gtgaaggttc gggctcgggc
     1381 tccggctcgg gttccgacga gcccgcaccg aagctcgacg cgaccggcac ctaccgcctc
     1441 aacagcacgt tcgatatcgc gacgaacatg ccgggcagct cgggctcgtt cgtcaacggc
     1501 ctgatcgccg ccaccgacga tccggacgat ccgatgtcgt ggctgctcga tcagatgctc
     1561 gaccagatgc caagcggcac gctcaagtcg atcctccagg gcgccaaacc gatcgtcgcc
     1621 ggctacctca acgaccagct cacgcagctc gcgcccgacc tcgtcggcac gatcctcgag
     1681 ctcggccatc acatggaaga catgacgaag cacttcggcg tcaacgagaa gctcgtgatc
     1741 gccggtgccg accagacgct cctcgccacg ctaaccgcgg acggcgtgcg cttcacggtc
     1801 gacgcaaacc agatcgatgt cgcgttcgcc gactacaacg tcgacgacgt cgtcgcgagt
     1861 ggcgtgctga tccagatcga gaacgagtcg aagatgctga tcggtgagca cgccctgccg
     1921 cttccctacg gcaagatcgt tcgcatgggc ctcgacacgg cgatcatccc gacgatcgat
     1981 ccgaacgccc acaacctcgc tgacctgctc ggcgacgtcg tcgactgcac gtcggtcggt
     2041 cagggcatcg cggatgcgct cgacttcggt agtgcgtcgt tctggacgtc agcctgcacc
     2101 gcgggcctca acggcgcggc caacctggtc tacgaccaga tcgcggcgaa ggactcgctg
     2161 ctgacgttcc acctcacggg caacgcgcgc gtgtcggacg gaaacggcga ccacaagctc
     2221 gacaagatcc agttcggcac gtgggcgggc acgctcagct acgacgcgac cgacacggcg
     2281 ctggtgcaac ccgcgtcgtt tgacggccac cgcgagtaac ctcgggcctc gctaacacgt
     2341 ccggcgcttc gcgccacgcg tgatacgtca ggcgcttcgc gccagcgttc tgcagagcgc
     2401 gaacgcgcgc ggctgcaacg gcacgcgcgc ccccgctcgg gtggattcgc gcctactcgc
     2461 cgcggcaacc tccttcgcag cggctctgcg tctcgagctg catcgttcgc gttgcttcgc
     2521 aacgcttgtg ccttcctgcg gcggcgcgct cgcggctcgc tgacggcgct cacaccctcg
     2581 gagggcaacg cgtgcctgcg cgccgcgcgc ttcgagactg caacgccgca acgtcgaaac
     2641 atcgcgctct gcacgcacga gcgtttcagc agaacgcgat ctggaatgtt tttggccttg
     2701 gcgttcttgg cgttcttggc atcttggcgt ccctctgggc gcgaaaccga tgacgcgcgg
     2761 acgcgggccg gcgagcagac gcgttggtcg cccgaagatc agcgcaggag gatcgcgtcg
     2821 ccttcgacgg cgacaaccgg tgcgctctcg gggtcgaagc gctcggcctt gaactcgcgg
     2881 tccgggccct cgacggtgtc gccgggctcg atgcgttcgc ccgacgcaag cgtcgcggcg
     2941 atgtcacgga gcaagagcgc ggcttcggtc gcgctttcgg gcgtgaggcc gagcagcacg
     3001 atgtcgggac ggccgaactt gcgcaggccg agcgaccacg cggcaatcga gccgtcgtcg
     3061 ctctcgtcga agaacagcgt gacctcgtgc atgatgtcga agctgcgttc gggcgagagc
     3121 gcggcgactt cgctgccgag gtgcgcacgg gcggcgtaga cgtcgatcac gacagtcgcg
     3181 ccttcttcgc agaggcactt ggctagcgcc caggcggcct ggagatgccc gaggtcatcg
     3241 ggatcgtcgt gctgcgcctc gatgacgtag gcgtgctcgg cggcgagtgc cgcggccgcg
     3301 tgctgcgggc cgaggtcggc cgcaatgaga tccgcgaacg gcgcgatgac ggattcggtg
     3361 aaccaggccg gatcttcagc gcggtcacgc gcgacgaggg cgacggagtt ggcgccggag
     3421 ccttcgtcgg gcatgccgtg ctgcttgcgc gagaccggca gcgggtccgg cattttttgg
     3481 gtggagagcg cgatgagggc gatctcgccc ggcccaccgc cgacgacgta gttcgcgcgt
     3541 ggccagacgg caaccatcag gccacgacgt cttcggtgta catgctgtcg atctcgcggg
     3601 cgtacttttg c
//
LOCUS       DASUFX010000359         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1445292, whole genome shotgun sequence.
ACCESSION   DASUFX010000359 DASUFX010000000
VERSION     DASUFX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445292"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFV99_14245"
     CDS             complement(<1..293)
                     /locus_tag="VFV99_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060523.1"
                     /translation="MTRISILFVAVALFAGCKKKDDGGTAAGKTTEAKATGPTKLPKL
                     GLSIDVPGAVEVGDAIMGEGHMLQGANVGALTVEIDDKPQTIDEAKSDADMYSP"
     gene            430..738
                     /locus_tag="VFV99_14250"
     CDS             430..738
                     /locus_tag="VFV99_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060524.1"
                     /translation="MSPEGLQIFAVVFAVWGVAVAIKAFNCLRNNEDYVFSMWDGGMI
                     RAGKRLNKMGKQIKLVVGVLEAVGCIGLLTGAIPLQTACYLIIFVAIVSLVSDFVTAA
                     "
     gene            735..896
                     /locus_tag="VFV99_14255"
     CDS             735..896
                     /locus_tag="VFV99_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060525.1"
                     /translation="MTDVPFAESLCHRCVNLRVVRSKTSTFLMCQEPSLPKYPRQPVV
                     ACGKFSPRP"
     gene            complement(880..1536)
                     /locus_tag="VFV99_14260"
     CDS             complement(880..1536)
                     /locus_tag="VFV99_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060526.1"
                     /translation="MAMWLRALLRGGGVSCAPRTLTAMSRYDAGVEPALIALLLTIGG
                     GITGYFAHAWYGSDARRTRRVLRRTRVTPIAELRDGRLACIVGHVEAEGELVQSLMSR
                     KDCVAFETVVQVFGSLDMTVPARVEVTRKLVTFFVVDDSGRARIDAAEAALANRAIMK
                     SERFEERVIENGARVRIVGSVVLDPTMQPDAEHAYRQGAFKATLTGTRKYPLLITVED
                     "
     gene            1621..2301
                     /locus_tag="VFV99_14265"
     CDS             1621..2301
                     /locus_tag="VFV99_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060527.1"
                     /translation="MARPHIRKLITWCCTVPASVVLVICLFLPQVQDCNGRTKTAFDT
                     NTWPFMVVLAIIGVLPIAWQWRPLRQPILLMIGVATASVLIMSVLGIPVLIALALTRS
                     MTDEEAVALCSFTLVLAFVLMFPVAMMFGDWRRGAELTWAAAWLELFGTIWWASAATE
                     LRPLQLRRWHFIRPRKLVQKADQVDDLRQQDVHRGAGGGEVLGRELLRVEPARQCQCI
                     HRASDGAP"
     gene            complement(2546..3409)
                     /locus_tag="VFV99_14270"
     CDS             complement(2546..3409)
                     /locus_tag="VFV99_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060528.1"
                     /translation="MRRLCLLISLTACKASISEGMDDAGAGSDAPRADALPLGPWGPP
                     EPIGITPVGDDDPTATGDLLELYFNRSSDIFVTKRSSPTGGWSTPTAVAELNSADAET
                     TPEVSYDGLTIYFASARMPTLGGNDVWVSTRASRADAWGTPVHVTDLSSVAADGGATT
                     VDATIAMFDSDRAGTTTLDIYMSQRASATAAWSTPALVPELESASNEGNPMLAADKLT
                     VYFDSNRGGDGELYAATRTTEAAPFAPPARIVELSSTAADTDPWISPDGRTMYFTSDR
                     DGTLRLWQTTR"
     gene            3452..3853
                     /locus_tag="VFV99_14275"
     CDS             3452..3853
                     /locus_tag="VFV99_14275"
                     /inference="COORDINATES: protein motif:HMM:NF022769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HEX5060529.1"
                     /translation="MGIEATGKLHTIFETKQVSERFSKREFVVELADNPKYPQTVLFQ
                     LTGDRVSQLDGMNVGDQVRIEFSLRGREWRSPSGEVKYFNSLDVWKIEPARAGAGRGR
                     RDSGADPRDIDMPPRIDESQFGRGDTDDIPF"
BASE COUNT          634 a         1307 c         1341 g          725 t
ORIGIN      
        1 ggcgagtaca tgtccgcgtc ggacttcgct tcgtcgatcg tctgcggctt gtcgtcgatc
       61 tcgaccgtca gtgcgccgac gttggcgccc tggagcatgt ggccttcgcc catgatcgcg
      121 tcgccgacct cgaccgcgcc cggcacgtcg atcgagagac cgagcttcgg gagcttcgtt
      181 ggaccggtag ctttcgcttc ggtcgtcttg ccggcagcgg tgccaccgtc gtccttcttc
      241 ttgcaaccag caaacagcgc gacagccacg aacaggatcg agatgcgagt cattgatgcc
      301 tccgtcccgc ggcatatttc acgtcgcgta ccgacgacgg cgcgcgcgat gtccgctact
      361 tagcggccgc ggcgggtcgg cacgaaccca attgccaaac ccgggtgggt ccggtgtagg
      421 gtgcgcgcca tgtcgcccga aggtctgcaa atttttgcgg tggtgtttgc ggtgtgggga
      481 gtcgcggtcg cgatcaaggc attcaattgc ctgcgcaaca acgaggacta cgtgttcagc
      541 atgtgggacg gcggcatgat tcgcgccggc aagcgactga acaagatggg caagcagatc
      601 aagctcgtcg tcggcgtgct cgaggccgtc ggctgcatcg gcctcttgac cggcgcgatt
      661 ccgctgcaga ccgcgtgcta cctcatcatc tttgtcgcga tcgtcagtct cgtatcggac
      721 ttcgtcaccg cggcatgacc gacgtcccgt tcgcggagtc gctgtgtcat cgctgcgtga
      781 acctccgcgt cgtgcgtagc aagacgtcga cgtttctgat gtgtcaggag ccgtcgctgc
      841 cgaagtatcc gcgccagccg gtcgtcgcgt gcggcaagtt cagtcctcga ccgtgatgag
      901 cagcgggtac ttgcgcgtgc cggtgagcgt cgccttgaac gcgccctggc gataggcgtg
      961 ttcggcgtcc ggctgcatcg tcggatcgag caccacgctg cccacgatgc gcacgcgcgc
     1021 gccgttctcg atcacgcgct cctcgaagcg ctcgctcttc atgatcgcgc ggttcgcgag
     1081 cgcggcttcg gcggcatcga tgcgcgctcg gccgctgtca tcgacgacga agaacgtcac
     1141 cagcttgcgc gtgacctcga cgcgcgccgg caccgtcatg tcgagactgc cgaagacctg
     1201 gacgacggtc tcgaatgcga cgcagtcctt ccgcgacatc agcgactgga cgagctcgcc
     1261 ctccgcctcg acgtgaccga cgatgcacgc gagcctgccg tcgcgtagct ctgcgatcgg
     1321 cgtgacccga gtgcggcgca gcacgcggcg cgtacgccgt gcatcggagc cgtaccacgc
     1381 gtgggcgaag tatcccgtga tgccgccgcc gatcgtcaga agcaacgcga tcagcgccgg
     1441 ttccacaccg gcatcgtaac gcgacatcgc cgtcagcgtg cgtggtgcgc acgagacgcc
     1501 accgccgcga agcagggcgc gtaaccacat cgccacgtct tgcgcatcgc gcgtctacgc
     1561 accggcgctg cgcggttcgt cacgcgaggc ggcggcatga ccgctgctcg atggcactgc
     1621 atggcccgtc cccatatccg caagctcatc acgtggtgtt gcaccgtccc cgccagtgtc
     1681 gtgctcgtca tctgcctgtt cttgccgcag gtacaggact gcaacggacg cacgaagacg
     1741 gcattcgaca cgaacacctg gccgttcatg gtcgtgctcg cgatcatcgg tgtgttgccg
     1801 atcgcgtggc agtggcggcc gctgcgccag ccgatcttgc tgatgatcgg cgtcgcgact
     1861 gcgagcgtgc tcatcatgag cgtgctcggg attccggtgc tgatcgcgct cgcactcacg
     1921 cgcagcatga ctgacgagga agcggtcgca ctgtgcagct tcacgctcgt gctcgcgttc
     1981 gtgctgatgt tcccggtcgc gatgatgttc ggcgattggc ggcgcggcgc cgagctgacc
     2041 tgggccgctg cgtggctcga gctgttcgga acgatctggt gggcgtcggc ggcgaccgag
     2101 ctacgtccgc tccagctgcg gcgctggcat ttcatccggc cacgcaagct cgtgcagaag
     2161 gccgatcaag tcgacgatct ccgtcagcag gatgtgcacc gtggtgcggg cgggggcgag
     2221 gtcctcgggc gcgagctgct tcgcgttgag ccagcgcgac agtgccagtg catccaccgc
     2281 gcgagtgatg gcgctccata gatcggcgtt gtgcacaccc agcgatgcgc gatcgtgaag
     2341 cgcggcgtgc agcgcatcga tacggcgctt cgcgttcgtg cggtgcctgc gcgcgcggaa
     2401 gagccgccag cgcaacagcg gtgcggtgag cgtgcggcgc agatcgagca ggtctttgcc
     2461 ccgcatcgcg gcggcttcgt cgagcacgaa cgagcacgac aagaggtcgt gccgcgcggc
     2521 ttcggcgatc tcgtgcagca tcgtgctacc gcgtggtttg ccacaatcgc agcgtgccgt
     2581 cgcgatcgct cgtgaagtac atggtacggc cgtcgggcga aatccacgga tcggtatcgg
     2641 ccgcggtgct cgacagctcg acaatgcggg caggcggagc gaacggcgcg gcctctgtcg
     2701 tgcgggttgc agcgtacagc tcgccatcgc caccgcgatt cgaatcgaag tagacggtga
     2761 gcttgtcggc cgcgagcatc gggttgcctt cattgctcgc gctctcgagc tcgggcacga
     2821 gcgcgggcgt gctccacgcg gcggtcgcgg atgcgcgttg cgacatatag atgtcgagcg
     2881 tcgtggtgcc ggcgcgatcg ctgtcgaaca tcgcgatcgt cgcatcgacg gtcgtcgcgc
     2941 cgccatcggc tgccacggag ctgagatcgg tgacatgcac aggtgtgccc caggcatcgg
     3001 cgcgcgatgc tcgcgtcgac acccagacat cgttgccacc gagcgtcggc atgcgcgcgc
     3061 tcgcgaagta gatcgtgagg ccgtcgtagc tgacttccgg cgtggtctcg gcgtcggcgc
     3121 tgttgagctc ggcgaccgcg gttggcgtgc tccagccacc ggtcggcgat gaccgcttcg
     3181 tcacgaagat gtcgctgctg cgattgaagt agagctcgag caagtcgccg gtcgcggtcg
     3241 gatcgtcatc gcccaccggc gtgatcccga tgggctccgg cgggccccac ggcccgaggg
     3301 gcagggcatc ggcacgcgga gcgtcactcc cggcgccggc gtcgtccatc ccttcgctaa
     3361 tgctcgcctt acaggcggtc agcgaaatga ggaggcagag ccggcgcacc ctctcagtct
     3421 atcagtcaga ccctctgcgt atcgtcgaga aatgggaatc gaagccaccg gaaagctcca
     3481 cacgattttc gagaccaagc aggtgtcgga gcggttttcc aagcgcgagt ttgtcgtcga
     3541 gctcgccgac aacccgaagt atccgcagac ggtgttgttc cagctcaccg gcgatcgcgt
     3601 ctcgcagctc gacggcatga acgtgggaga ccaggtgcgc atcgagttct cgctgcgcgg
     3661 tcgcgagtgg cgcagcccgt cgggcgaagt gaaatacttc aacagcctcg acgtgtggaa
     3721 gatcgagccc gcgcgtgctg gcgccggtcg cggccgccgc gacagcggtg cggatccgcg
     3781 cgacatcgac atgccgcctc gcatcgacga gtcgcaattc ggccgcggcg acaccgacga
     3841 cattccgttc tgagcgacac cgcattctgc gcgagccggc acaaccgtcg cgcgcgtgaa
     3901 ccgcgatcgc accggctcgt tacgtcacta aacgtagtcg ggtgcgagtg agcgcagttt
     3961 ctgtcgagtc cacgcgcacg ctgcgcgctc ttgccgcacg ccgagaa
//
LOCUS       DASUFX010000360         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_56836,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000360 DASUFX010000000
VERSION     DASUFX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56836"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFV99_14280"
     CDS             <1..734
                     /locus_tag="VFV99_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060530.1"
                     /translation="TPQPEPPPSLPNCVPNRDGVITADEMPVVFDASLNYYVGTNVTI
                     AQTGERWDYSTERAEDDVVALGPVALKDQWYASNFPAGQFVVDAGSGLEGIYHQDAQA
                     LWLDGTASHDMAMASRTLVVYPQAVAVLRFPVAAGDMYTTRATLAGATINGLPFNGYD
                     DITVSVVGEGLLAVPYVEFSPVLRVTVAVARVPTSGPSTSKRTTLFLFECFGEVARAE
                     SKADEPSADFSFAAYFRRFALGVTP"
     gene            731..1021
                     /locus_tag="VFV99_14285"
     CDS             731..1021
                     /locus_tag="VFV99_14285"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEX5060531.1"
                     /translation="MIWDMWKQGFAAWEQATAQYFEKVLTNPGVLGPAGAMLTAAMKT
                     KTATDKALAAWWSSFGLPTRREQERALHKLNQLESRLYDLEEQLLMQSSKRN"
     gene            1024..2607
                     /locus_tag="VFV99_14290"
     CDS             1024..2607
                     /locus_tag="VFV99_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEX5060532.1"
                     /translation="MDVAHLLTVAPAPHAVFERLATHRDRARFHVRVGDAWQPVTWGQ
                     FAAQIRGVARWLVEHELQPGQRIAIYAPNSVAWAAAALGAQTAGGVFVPIYPASTPEQ
                     VAYILEHAEIKYVFVGGKELIARLEKARALLASPPQVVAVDDAAFRGATARDAAEPGF
                     VDTRLSALDLDQPAQMLYTSGTSGNPKGVPLTHRNVGTSGSDWLQCNAPLLAEGDRDL
                     LWLPMSHIFGFGELCAGNLLGWETYLATPADVLEHLPSVAPHVFMSVPAYWEKIARAV
                     ATHADRSEALRKVTGGNLRFCLSGGAGLKVEIKEQLHALGMLIIEGYGLTETSPTLTL
                     NRPDSFRFDTVGKPLPSLELKLDTDGEILARGPNVFAGYFKDPAATAAAFTEDGWFRT
                     GDVGQWTEDGFLKIVDRKKDILVNSGGKNIPPANIEAKFVDDPAIERVVVYGDARPYL
                     VAAVWMRPDVPADDRVRLAAKRIDEINTELARHETIKRHFIAELPLTVEDGTLTSSLK
                     LRRKVVYDRLRDRFEALYA"
     gene            2615..>3459
                     /locus_tag="VFV99_14295"
     CDS             2615..>3459
                     /locus_tag="VFV99_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5060533.1"
                     /translation="MQAPKKSPLDSIIALVKRAPKGQPAVGMTPHSVVWTENKWRLLR
                     FAPARPKYKTPVLLVPSLINRWYVLDLGTGRSLIEWLVAQGHDVFCIDWGTPGSEDRY
                     LTWDDFAGRYVGRAVRIASRYGRSGDVHLLGYCLGGTLATSYVAAFPERIASFLALAA
                     PIDFEHAGIMATWCRTPTFDVGAIFEAFGNIPWPLMQASFNMLRPTLRAAKTVALLDR
                     AWDDEFLEGFLATEHWGHDNVSFPGACYARYIEELYRANRLISGGFSVMGRPAELHQI
                     KCPVLA"
BASE COUNT          585 a         1149 c         1126 g          599 t
ORIGIN      
        1 tgacgccgca gcccgagccg ccgccgagct tgccgaactg cgtgccgaat cgcgacggtg
       61 tgatcaccgc ggacgagatg ccggtcgtgt tcgacgcgtc gctgaactac tacgtcggca
      121 cgaacgtcac gatcgcgcaa accggcgagc gctgggacta cagcaccgag cgcgcagaag
      181 acgacgtcgt cgcactcggt ccggtcgcgc tcaaggatca gtggtacgcg agcaatttcc
      241 cggcggggca gttcgtcgtc gacgctggta gcggcctcga gggcatctat caccaggacg
      301 cgcaggcact gtggctcgac ggcaccgcgt cgcacgacat ggcaatggcg tcgcgcacgc
      361 tggtcgtgta cccgcaggcc gtcgcggtgc tgcgcttccc ggtcgcggcc ggcgacatgt
      421 acacgacgcg cgcgacgctt gcaggcgcca cgatcaatgg cctgccgttc aacggatacg
      481 acgacattac ggtgtcggtc gttggcgagg gcctgttggc tgtgccctac gtcgagttct
      541 cgcccgtgct acgcgtcacc gtcgcggtcg cgcgcgtacc gacgagcggc ccgtcgacga
      601 gtaagcgcac gacgctgttc ttgttcgaat gtttcggtga ggtcgctcgc gcggaaagca
      661 aagccgacga gccatctgcc gattttagtt tcgccgccta tttcaggcga ttcgcgttag
      721 gagtgacacc atgatctggg acatgtggaa acaaggcttc gctgcttggg agcaagccac
      781 cgcgcaatac ttcgagaagg tgctgacgaa cccgggcgtg ctcgggccgg ccggcgcgat
      841 gttgaccgcg gccatgaaaa cgaagaccgc gactgacaag gcgctcgccg cctggtggtc
      901 gtcgttcggc ttgccgacgc gccgcgagca agagcgcgcg ctgcacaagc tcaaccagct
      961 cgagagccgg ctgtatgacc tcgaagagca actactgatg cagtcctcga agaggaacta
     1021 gccatggacg tcgcgcacct gcttaccgtt gcacccgcgc cgcatgccgt attcgagcgg
     1081 ctggcgacgc atcgcgatcg cgcgcgtttc cacgtgcgtg ttggcgacgc gtggcaaccg
     1141 gtcacgtggg gccagttcgc tgcccagatc cgtggcgtcg cgcgctggct cgtcgagcac
     1201 gagctgcaac ccggccagcg catcgcgatc tacgcgccga acagcgtcgc gtgggcagct
     1261 gctgcgctcg gcgcgcaaac cgcaggtggg gtgttcgtgc cgatctatcc ggcgtcgaca
     1321 cccgagcaag tcgcgtacat cctcgagcac gccgagatca aatacgtgtt cgtcggtggc
     1381 aaggagctca tcgcacgtct cgaaaaggcg cgcgctctgc tcgcgtcgcc gccgcaagtt
     1441 gtcgccgtcg atgatgcagc attccgaggc gccacggcgc gcgacgctgc agaaccggga
     1501 ttcgtcgaca cgcggctgtc ggctctcgat ctcgatcaac cggcgcagat gctctacacg
     1561 agcggtacat cgggcaatcc gaagggcgtg ccgctcacgc atcgcaacgt cggcacgagc
     1621 ggctccgatt ggctgcagtg caacgcgccg ctgctcgcgg agggcgatcg cgatctgctc
     1681 tggctgccga tgagtcacat cttcggattc ggcgagctgt gtgcgggcaa cttgctcggt
     1741 tgggagacgt acctcgcgac gcctgccgac gtgctcgagc acctgccgag cgtcgcgccg
     1801 cacgtgttca tgtcggtgcc tgcgtactgg gagaagatcg cgcgcgccgt cgcgacacac
     1861 gccgatcgca gcgaggcgct gcgcaaggtc accggcggca acctgcgctt ctgtctctcc
     1921 ggcggtgcag gcctcaaggt cgagatcaag gagcagctgc acgcgctcgg catgctgatc
     1981 atcgagggct acgggctcac cgagacgtcg ccgacgctca cgctcaaccg tcccgactcg
     2041 ttccggttcg acacggtcgg caagccactg ccgtcgctcg agctcaagct cgacaccgat
     2101 ggcgagatcc tcgcgcgcgg cccgaacgta ttcgccggct acttcaagga tccggcggcc
     2161 actgcggcgg cgttcaccga ggacggctgg ttccgcaccg gtgatgttgg ccagtggacc
     2221 gaggatggct tcctcaagat cgtcgatcgc aagaaggaca tcctcgtcaa cagcggcggc
     2281 aagaacatcc cgcccgcgaa catcgaggcg aagttcgtcg acgatccggc gatcgagcgc
     2341 gtcgtcgtct atggcgacgc gcggccgtac ctggtcgccg cggtgtggat gcgtcccgat
     2401 gttcccgccg acgatcgcgt gcgccttgcc gcgaagcgca tcgacgagat caacactgag
     2461 cttgcacgcc acgagacgat caagcggcac ttcatcgccg agctgccgct caccgtcgag
     2521 gacggcacgc tcacgtcatc actgaagctg cgccgcaagg tcgtgtacga ccggctgcgc
     2581 gatcgcttcg aggctctgta cgcgtaggat ggctatgcag gcgccaaaga aaagtccgct
     2641 cgattcgatc atcgcgctcg tcaagcgcgc gcccaaaggg caacccgccg tcggtatgac
     2701 gccgcactct gtcgtgtgga cggagaacaa gtggcgtctc ctccgcttcg cgccggcccg
     2761 cccgaagtac aagacgccgg tcttgctcgt gccatcgctg atcaatcgct ggtacgtgct
     2821 cgacctcggc accggtcgct cgctcatcga gtggctggtc gcgcaaggcc acgacgtgtt
     2881 ctgcatcgac tggggaacgc cgggctccga agatcgttac ctgacgtggg acgacttcgc
     2941 cggccgctac gtcggccgcg ccgttcgcat cgcgtcgcgc tacggccgtt cgggtgacgt
     3001 tcatctgctc ggctactgcc tcggtggcac gctggcaacc agctacgtcg ctgcgtttcc
     3061 cgagcgtatc gcatcgttcc tcgcgctcgc ggcgccgatc gatttcgagc acgcgggcat
     3121 catggcgacc tggtgccgca cgccgacgtt cgatgtcggc gcgatcttcg aggcgttcgg
     3181 caacatcccg tggccgctca tgcaggcgag cttcaacatg ctgcgcccca cgctgcgtgc
     3241 ggcaaagacc gtggcgctgc tcgatcgcgc atgggacgac gagttcctcg aaggattcct
     3301 cgcgaccgag cactggggcc acgacaacgt ctcgttcccc ggcgcctgtt atgcgcgcta
     3361 catcgaggag ctgtaccggg ccaatcgact gatcagcggc ggcttctcgg tgatgggtcg
     3421 gccggcggag ctacaccaga tcaaatgccc cgtgctcgc
//
LOCUS       DASUFX010000361        15453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1030758, whole genome shotgun sequence.
ACCESSION   DASUFX010000361 DASUFX010000000
VERSION     DASUFX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030758"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..962)
                     /locus_tag="VFV99_14300"
     CDS             complement(267..962)
                     /locus_tag="VFV99_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5060534.1"
                     /translation="MPELVARIVLSRSSEFALDVELTCPHGITCIMGPSGSGKSTILA
                     VLAGLQAPDKGRVALGNQVWLDRASGIDVPVHKRRLSYVFQGLALFPHMNALHNVEYG
                     MQHVPRMERAAKAKALLDRVGVGHLARRRPRTFSGGEAQRVALARALARSPELVLLDE
                     PFSALDRELRAQLVALVRDLSKDLAVPIVHVTHSIAEARQLADQIVRVDKGRVVARGK
                     ADEVLAGVTTLEE"
     gene            complement(955..1743)
                     /locus_tag="VFV99_14305"
     CDS             complement(955..1743)
                     /locus_tag="VFV99_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter permease"
                     /protein_id="HEX5060535.1"
                     /translation="MERASSLLDSLRAERGEIARFAITLAIVIGVIAYVQSQGGIVWE
                     PMALSLRIALLSMVLTLVVGTALTVLLMWKRLPARDLLDAITSVPLVLPPTVLGYYLI
                     VSLGTESAVGRAWQSVFGGQLIFTFKGAVIAASMGSLPLVVRSVRIGLESVDASYTNA
                     ARTLGAGPLRVFFTIVLPLAAPGVIAGGMLGFARALGDYGATMFFASSRIDGVPTASI
                     AVMDFFYGNHEAQARQMSFVMTVVGVGMLYLANRLTRRMHPNRA"
     gene            complement(1746..2504)
                     /locus_tag="VFV99_14310"
     CDS             complement(1746..2504)
                     /locus_tag="VFV99_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5060536.1"
                     /translation="MPLTKGPAGVRAETANDNSESAKELREKKRVLAALVVHDLRSPL
                     SAVQGYLHLLKEEIGPDAPPGVTQYLDDANVLVGKALGLVATILDVDELEDGILRAVP
                     QPVKLMELIDRARAGNRAHFEVRQLKLDLDIEHDMTIKLDRDLFGRVLENLLDNATRY
                     APRGGRCAISAKRMGAGVEIAIGNNGPPVPPADRDAIFGRFFQVERRRQSARANRGLG
                     LYFCKLAVEAHGGTIRVEERGDLGAVFVVTLPAA"
     gene            complement(2504..3388)
                     /locus_tag="VFV99_14315"
     CDS             complement(2504..3388)
                     /locus_tag="VFV99_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5060537.1"
                     /translation="MSDTGRNKTVVTAISKISDRPVAKEACLVVIYGLELGKKFNLNR
                     PQIIIGRSSKADIQIDQEAVSRNHCKIINTGNAIMLRDMGSTNGTYVNDEMIDEYVLR
                     DGDFIKVGRCIFKFLSGNNIENAYHEEIYRLTTVDGLTQVFNRRYFVETLEREIGRAL
                     RYRRDLSLIMFDIDRFKGVNDSFGHLAGDHVLKHLASVIKTRIRREDVLARYGGEEFA
                     IVLPEIDHYNAMQFAEKIRKLVEVAEFKFEDAVIPVTVSIGVASLRGEIEDALEFIKQ
                     ADTHLFSAKEGGRNRVVG"
     gene            complement(3438..3857)
                     /locus_tag="VFV99_14320"
     CDS             complement(3438..3857)
                     /locus_tag="VFV99_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060538.1"
                     /translation="MATVQPGTCAEPDRDGVMSESPRLEHADRDLDGDGQAELIVVDR
                     AKCSDDGNCYWNVFRRSRDPGECARYAGTFAGSALETLPTRGEDNMSDVRAFWKQTGN
                     RMLLQSYRFVRDGYRIEDVLQCKRAPDDRLDCADTNR"
     gene            complement(3940..5352)
                     /locus_tag="VFV99_14325"
     CDS             complement(3940..5352)
                     /locus_tag="VFV99_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5060539.1"
                     /translation="MSSNQLVLDATALTSAGIGDKGPDEHAISAVGDSARRAVETTLG
                     RRERGEIGFLDLPDDRTTARACMDFARTLDPFIDTVVTLGIGGSSLGPRALYSALAQP
                     YDHLATRSAGMPRRLFFPDNIDPITFAAIIERLPLDRVLWNVVTKSGATAETCAQFLV
                     VADQLDKRLGPERAKKHIVCTTDPEKGALRAVAAERGYPVFDIPQNVGGRFSVLSAVG
                     LLPAALAGLDVMGLLDGARRMRDRVLVPDVATNPALLLATLLHHHHIVRGRNLTVMMA
                     YVDGLYDTADWFRQLWAESLGKEKSTDGRTVNVGPTPIAARGATDQHSQLQLYAEGPD
                     DKVFLFVTAADRGGEVKIPGGALAESQAELSYLAGHGLGELLDAELTGTMSSLVRRGR
                     PCGKLTLAKVSAEAIGELLVLLEAITAFAGPLYGVDPFDQPGVEEAKRLAFAALGRPG
                     YEALALEMSKAPGPDPKYVL"
     gene            5417..6730
                     /locus_tag="VFV99_14330"
     CDS             5417..6730
                     /locus_tag="VFV99_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5060540.1"
                     /translation="MRRAVLLAGGALLAGAGLFIRSGAGPTPKASIDGEPIATAPKPI
                     EADSKATPEEIATYESLGADPIKQASVDLQLDKMELKDGHYEAPLKDGRRATLTLDPD
                     LQKLAEKLLNESRAPRGAIVAMTPDGKILAFAGRRTQENKGGKEGTFDYHLATDVWAP
                     AASVFKLVTASALLSNGFNPDDKVCYHGGVRSVMESNLTDSKSDGNCETLGYGVAHSN
                     NAILGKLAYQKLDPTKLDHQARDLGWTFAPSFGVKATCGELDLPKDKNLDFAKAAAGF
                     NGSRLSVLGGALLSAIFASGGQQPVPRIIESINGKPFAGQKAKRVLPETVAKTVATMM
                     QETCESGSAAKTFRKRSYEVAGKTGTLSTNKPFFMEHSWFVGFAPTDQPQIVISVLLG
                     NPESWHLRGHEAAKRMIDRAIRRAGDREENVETKSTKSSARASKR"
     gene            6773..7906
                     /locus_tag="VFV99_14335"
     CDS             6773..7906
                     /locus_tag="VFV99_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060541.1"
                     /translation="MSRLTFGLVLASSLLGGVALAESQAEIAARLNEEGKQLLFAEKY
                     AEASAKFQDAHSRVPEPKYFYNHCMSLTAEGKFGEALTACNAADKNADASLKDKIAKL
                     ETRIHEEAKKQGIDLQPVGGGGGPGDTPPVDPNNPNQPPVDPNNPNNPPPGGQPPAGP
                     GMAVGRPVGNVLFQAAPENKYTWSLGFDLYGGSGQVGRSGYFGKATGGIRVKGDYLFN
                     PAKRIGTQAYFQLTHFGEGDDMASTGALSLDVFDLGIAGYKHLCGPHARGCITPLIGV
                     QLALMSPADQMDASTGDQVFNYASVGARLELALSYALGSHFEHVLSLTGGLNAYTKVF
                     SEPSEGDSAAFWGLDKGGAAGYIGIGYTYRFNTPFGTSPFVVL"
     gene            8074..9366
                     /locus_tag="VFV99_14340"
     CDS             8074..9366
                     /locus_tag="VFV99_14340"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX5060542.1"
                     /translation="MAEKLSAKLILPGGKENQLPVIVGTEDEHAVDIRNLRQETGYIT
                     LDSGYMNTGSTTSAITYLDGEQGILRYRGYPIEELAEHSDFVETSYLLINGELPNRAE
                     REAFRTKLTRHSLIHEDMHHFFAGFPPTSHPMAVLSAMVTSLSAYYPDSLERNAPSDL
                     HITRILSKVRTIAAFAYKKSIGQPIVYPKNALRYCANFLHMMFAVPSEDYEVSPVLEK
                     ALNQLLILHADHEQNCSTSTVRMVGSSGANLYAAIAAGILALWGPLHGGANQAVIEML
                     EEIKASGGDHKKFIDDVKNKRENRRLMGFGHRVYKNYDPRAKILKKMCDEVLVHLGIK
                     DPLLDIAKNMEEIALKDDYFVSKKLYPNVDFYSGIIYRAMGIPTNMFTVMFALGRLPG
                     WIAHWKEMVEDPQTKIGRPRQIYTGPKQRKYVPLENRA"
     gene            9366..9911
                     /locus_tag="VFV99_14345"
     CDS             9366..9911
                     /locus_tag="VFV99_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060543.1"
                     /translation="MRRSAVVVALVVMASACGKNESSPAASGSAAGSAAANDPWSKPA
                     APAPAPAAAPCDDADIKKHIDDSLAVSLAYMAALEKKALKWKTDCSVAKKDLLALEPE
                     ATKFMASVNDFMTWGKTLTPECGKRVAEIGEQYDAARDIEKRTPELEAHVKTILEHCK
                     DYPGFQDAAAKGLRVMHKKKP"
     gene            complement(9912..10571)
                     /locus_tag="VFV99_14350"
     CDS             complement(9912..10571)
                     /locus_tag="VFV99_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5060544.1"
                     /translation="MRSFTHSPATIIAVLAMALVACKPKYSDYPPPYTRTAHGQTAAA
                     RPAPAKGGKPTSAPKSEPPKPEPPKPQHLDPFAQQFIDAHNEVRAKHCAGPLTWSKKL
                     ADIAQNWANTLKSKGCVFGHSPGAQYGENLAAGTVGALDPTSTVAMWYDEIKLYKFPN
                     GGFSARTGHFTQVVWRSTQQVGCGHVECNGNDIYVCTYDPAGNWEGQYRQEVLPASCK
                     K"
     gene            complement(10579..11052)
                     /locus_tag="VFV99_14355"
     CDS             complement(10579..11052)
                     /locus_tag="VFV99_14355"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5060545.1"
                     /translation="MAKKKALATDKVLQELRAFGMQYPGAHFKSPWPGHMDLAVNDKT
                     FAYLSNEGEPFGISCKLPQSAAVALMLPFCSPAAYGLGKAGWVSAKFPEGKALPVEML
                     KEWLDESYRAQAPKKFLKQLEGGAAAPATVNKRKPVTKRTAAAKTTKRAAKKRVS"
     gene            11075..11494
                     /gene="apaG"
                     /locus_tag="VFV99_14360"
     CDS             11075..11494
                     /gene="apaG"
                     /locus_tag="VFV99_14360"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX5060546.1"
                     /translation="MPGPMVAESTSQALTQGVRVRVQSMYLPDQSSPNDDRYVFAYTI
                     TISNESTFTAQLRTRHWIITDGRGGVEEVKGDGVVGEQPRLSPGQSFQYTSGCVLTTP
                     IGTMHGTYRFWRDDGTYFDAVIAPFSLASPVRDQEAN"
     gene            11575..12234
                     /locus_tag="VFV99_14365"
     CDS             11575..12234
                     /locus_tag="VFV99_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5060547.1"
                     /translation="MTNAEREAATATALVAAAKAGDTRAFEALVKRYRKRIYALALHM
                     TRSASEADDITQEVFLKAYRALGQFEGRSQFFTWVYRMTVNRSLNAKRDRARRGEDTI
                     DDPRLELAVAVDARGNPGREAELRQSYARLLRALDQLPIDMRTTVILVSLQGMSHAEV
                     GIIQKVSDGTIAWRMHEARRRLHDAMAPEKVTRKRGELSAELSRALSEYGLLVPGKAR
                     A"
     gene            complement(12231..12890)
                     /locus_tag="VFV99_14370"
     CDS             complement(12231..12890)
                     /locus_tag="VFV99_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5060548.1"
                     /translation="MLTVHHLNGSRSQRVLWLLEELGVPYEIVKYERDPKTDLAPPEL
                     DKIHPLGKSPVITDGDVTIAESAAILEYIVEKYGNGKMQPPKDTPNHRRYRYFMHYAE
                     GSLMPFLLLRLITSKLKRTPFLVRPIAKAIAGKLEGSYVAPNLDRHVAFLASELGTRD
                     YFAGGEITAADIQMSFPMEALALRVKDVPDAIKAWVTRMHERPAFKKAVERGGPYDLM
                     T"
     gene            12921..13436
                     /locus_tag="VFV99_14375"
     CDS             12921..13436
                     /locus_tag="VFV99_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5060549.1"
                     /translation="MRLLLATLLLAGCAQLMPAAKQEPFPVMEVKATRPPPGPARVVL
                     TESNIAIMDKVQFETGKANLLPASNALLDEVAKMLKDNPQVELISVEGHTDSTGSPDF
                     NRKLSQQRAESVAKYLSGKGVKAARMEPKGFGPDRPIADNETDQGREANRRVEFNILK
                     QGPKKVLVKDE"
     gene            13429..13803
                     /locus_tag="VFV99_14380"
     CDS             13429..13803
                     /locus_tag="VFV99_14380"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5060550.1"
                     /translation="MNKLTLFIAAVSLSALAGACAKQGMTPAVRNEITAKLQSTQTPI
                     QQCYARQLATNRKLAGMVVAQAAIAPDGQFGEVSLRRDEPADPVLRFCVVQEIAKLKL
                     DKPTGERVVLDSIPIKFVWANP"
     gene            13788..14243
                     /locus_tag="VFV99_14385"
     CDS             13788..14243
                     /locus_tag="VFV99_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060551.1"
                     /translation="MGEPVSKTPTRSPTHEDVDPTRLAGMGLEIEDIADGDTVALPAL
                     VELAEDRGRPVSHYLAAIPLATELRLAGTGPLTLKICAGTCQRWGALDLLDHVAERVT
                     KGAKLTLAPVSCLDRCDQAPACEIHGPHGQLVVAPATAATLDEAIDSLS"
     gene            14317..15051
                     /locus_tag="VFV99_14390"
     CDS             14317..15051
                     /locus_tag="VFV99_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5060552.1"
                     /translation="MLRLNKVNKYYQVGTHSLHVLRDVELEVADGEFVAIMGASGSGK
                     STLLNILGILDDYDSGEYWLGDTLIRKLSETRAAEYRNRFIGFVFQSFNLLPFKTALE
                     NVALPLYYQGVTRRKRNQIAMQYLERVGLRDWADHVPSEMSGGQKQRVAIARSLIGKP
                     RLILADEPTGALDSETSRQIMDLMSEINSTGITVLVVTHDPEVASRTQRTIRLRDGKV
                     LSAHEAALSNPGMPAARDSIPPSAGT"
     gene            15052..>15453
                     /locus_tag="VFV99_14395"
     CDS             15052..>15453
                     /locus_tag="VFV99_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060553.1"
                     /translation="MAIAALKLLGILAGLALAVTLIVVVVIVLIAIVRMAIVGVVQWR
                     DLFSLDRWSEVLITIGRNKLRTALTTISVAWGIFVLVILLGLGNGLNNGAHHEFARDA
                     TNGVWITGNKTSEAFGGYDIGRRIKFDNRDYD"
BASE COUNT         2827 a         5066 c         4962 g         2598 t
ORIGIN      
        1 cgcgcgttgc tgcatcgagt agcgcatgga cgatcacctg cgtgatcgtc cgcggccgcg
       61 ctcccgcggg aagccccttc gtgcgcagtt tctccagcgc gcgagcctcg tacggtgaca
      121 gacggatcgt gatcttcgac agctgtctgc gatcttccat gacctctacg tcgcccttcc
      181 ggtgtcgcgt acagactagg cagttcgcgt tcggcgtcaa tgttccacgg gcgttgatca
      241 cgtgaacaga tcgcagcgag gtcgcgtcac tcttcgagcg tggtgacgcc agcaagaact
      301 tcgtcggcct taccgcgcgc gacgacgcgg cccttgtcga ctcgcacgat ctgatcggcg
      361 agctgacgcg cttcagcgat cgagtgcgtg acatgcacga tcggtaccgc cagatctttc
      421 gaaaggtcgc gcacgagagc aacaagctgc gcacgcagct cgcgatcgag tgcggagaac
      481 ggctcatcga gcaacacgag ctccggtgag cgcgcgagcg cacgtgcgag cgcgacacgc
      541 tgcgcctcac ctccggagaa cgtgcgcggc cgccgccgcg cgagatgacc gacgccgacg
      601 cgatcgagca acgccttcgc cttcgccgcg cgctccatgc gcggcacgtg ctgcatgccg
      661 tactcgacgt tgtgcaacgc gttcatgtgc gggaacagcg cgaggccttg gaacacgtac
      721 gagagacgac gcttgtgcac cggcacgtcg atgccactcg cgcgatcgag ccagacctga
      781 ttgccgagcg cgacgcggcc cttgtcggga gcttgcaggc cggcgagcac ggcgaggatc
      841 gtgctcttgc ccgagcccga cgggcccatg atgcaggtga tgccgtgcgg gcatgtcagc
      901 tcgacatcga gcgcgaactc gctcgaccgc gacagcacga ttctggcgac gagctcaggc
      961 acggttcggg tgcatccgcc gggtgagccg gttcgcaagg tagagcatgc caacgccgac
     1021 gacggtcatg acaaaggaca tctgacgtgc ctgcgcctcg tgattgccat agaagaagtc
     1081 catgaccgcg atcgaggccg tcggaacccc gtcgatgcga gagctggcga agaacatcgt
     1141 tgcgccgtag tcgccgagcg cgcgcgcgaa gccgagcatg ccgcctgcga tcacgcccgg
     1201 cgcggcgagc ggcagcacga tcgtgaagaa cacgcgcaac gggccggcgc ccagggtgcg
     1261 cgccgcgttc gtgtagctcg catcgaccga ctcgagcccg atccgcaccg atcgcacgac
     1321 gagcggtagc gagcccatcg acgccgcgat gaccgcgccc ttgaacgtga agatcagctg
     1381 gccgccgaac accgactgcc acgcccggcc gaccgcgctc tcggtgccga gcgacacgat
     1441 gaggtagtag ccgagcaccg tcggtggaag cacgagcggc accgacgtga tcgcgtcgag
     1501 caagtcgcgc gccggcagcc tcttccacat cagcagcacg gtcagcgcgg taccgacgac
     1561 gagcgtgagc accatcgaga gcagcgcgat ccgcagcgac agcgccatcg gctcccacac
     1621 gatgccgccc tgcgattgca cgtacgcgat cacgccgatc acgatcgcga gcgtgatcgc
     1681 gaagcgtgcg atctcgccgc gctctgcccg tagcgagtcg agtaacgagc ttgcgcgttc
     1741 catggttatg ccgccggcag cgtcaccacg aacaccgcgc ccagatcgcc gcgctcctcg
     1801 acacgaatcg tgccgccgtg ggcttccacg gcgagcttgc agaagtagag cccgaggccg
     1861 cgattcgcgc gggcactctg gcggcggcgc tcgacctgga agaagcggcc gaagatcgcg
     1921 tcgcgatccg cgggcggcac cggcggaccg ttgttgccga tcgcgatctc gacgccagcg
     1981 cccatgcgct tcgcactgat cgcacagcga ccgccgcgcg gcgcgtagcg agtcgcgttg
     2041 tcgagcaggt tctcgagcac gcggccgaac agatcgcgat cgagcttgat cgtcatgtcg
     2101 tgctcgatgt cgagatcgag tttgagctgg cgcacctcga agtgcgcgcg attgccggca
     2161 cgcgcgcgat cgatcagctc catcagcttc accggctgtg gcaccgcgcg caggatgccg
     2221 tcctcgagct cgtcgacgtc gaggatcgtc gcgacgaggc cgagcgcttt gccgacgagc
     2281 acgttcgcgt cgtcgagata ctgcgtgacg ccgggcggcg cgtcgggacc gatctcttcc
     2341 ttgagtaagt gcagatagcc ctgcactgcc gacagcgggc tgcgcagatc atggacgacc
     2401 agtgccgcga gaacgcgctt cttctcgcgc agctccttcg cgctctcgct attgtcgttc
     2461 gccgtctccg cacgaacgcc ggccggccct ttcgtcaacg gcatcagcca accacgcgat
     2521 tacgcccgcc ctctttggcg ctgaacagat gcgtatcggc ttgtttgatg aactccagcg
     2581 cgtcctcgat ctcgccgcga aggctcgcaa cgccgatcga tacggtgacc ggaatcaccg
     2641 cgtcctcgaa cttgaactcc gcgacctcga cgagcttgcg gatcttctcc gcgaactgca
     2701 tcgcgttgta gtgatcgatc tcgggcaaca cgatcgcgaa ttcctcgccg ccgtagcgcg
     2761 cgagcacgtc ttcgcgccgg atgcgcgtct tgatcaccga cgcgaggtgc ttgagcacgt
     2821 gatcgccggc gaggtgaccg aagctgtcgt tgacgccctt gaaccgatcg atgtcgaaca
     2881 tgatcagcga caggtcgcgg cgatatcgca acgcacgccc gatctcgcgc tcgagcgtct
     2941 cgacgaagta gcggcggttg aacacctgcg tgagaccgtc gaccgtggtg aggcgataaa
     3001 tctcctcgtg gtaggcgttc tcgatgttgt tgccgctgag gaacttgaag atgcagcgac
     3061 cgaccttgat gaagtcgccg tcgcgcagca cgtactcgtc gatcatctcg tcgttgacgt
     3121 aggtgccgtt ggtcgacccc atgtcacgca gcatgatcgc gttgccggtg ttgatgatct
     3181 tgcagtgatt ccgcgagacc gcttcctgat cgatctggat gtcggcctta ctcgagcggc
     3241 cgatgatgat ctgcgggcgg ttcaggttga acttcttgcc cagctcgagg ccgtaaataa
     3301 caacgaggca cgcctctttc gcgaccggtc gatcgctgat cttcgagatc gcggtgacga
     3361 cggtcttgtt acggccggta tcgctcaccc agcgttgatc ctatcaagaa atccggacct
     3421 ttgcgcgtct tgcgctgtca cctgtttgtg tcggcgcaat cgaggcggtc gtcgggtgcc
     3481 cgcttacact ggagcacgtc ctcgattcga tagccgtcgc gcacgaaccg gtaggactgc
     3541 agcagcatgc gattcccggt ctgtttccag aacgcccgca cgtcgctcat gttgtcctcg
     3601 ccgcgggtcg gcagcgtctc gagcgccgaa ccggcgaacg tacccgcgta gcgcgcacac
     3661 tcaccgggat cgcgcgacct ccggaacacg ttccagtagc aattaccgtc gtcgctgcac
     3721 ttcgcgcgat cgacgacgat gagctccgcc tgaccgtcgc cgtcgagatc gcgatcggca
     3781 tgctcgagcc gcggcgactc gctcatgacg ccgtcgcggt ctggctccgc acacgtcccc
     3841 ggttgcacgg ttgccatcgg catccgcacc gtcgtgggcg ccggcttact gcgcgtacac
     3901 gcgccgatga cgacactggc gacgacgaaa agaatgcgcc tagaggacat acttcggatc
     3961 cggtccgggt gccttggaca tctcgagtgc gagcgcttcg tatcccggcc gaccgagcgc
     4021 ggcaaacgcg agtcgctttg cctcttcgac gccgggttgg tcgaacgggt cgacaccgta
     4081 gagcggtcct gcgaacgcgg tgatcgcttc gagtaacacg agcagctcac cgatcgcttc
     4141 cgccgacacc ttcgcgagcg tgagcttgcc gcacggacga ccacgccgca cgagcgagga
     4201 catcgtgccc gtgagctcgg catcgagcag ctcgccgagg ccgtgacctg cgaggtagct
     4261 gagctcggcc tgtgactctg cgagcgcacc gccgggaatc ttcacctcgc cgccgcgatc
     4321 ggccgcggtc acgaacagga agaccttgtc gtccggtcct tccgcgtaga gctgcagctg
     4381 actgtgctga tcggtcgcgc cgcgtgccgc gatcggtgtc gggccaacgt tgaccgtgcg
     4441 gccgtccgtc gacttctcct tgccaaggct ctccgcccac agctggcgaa accaatcggc
     4501 ggtgtcgtag aggccgtcga cgtacgccat catgacggtg aggttgcgac cgcgcacgat
     4561 gtgatggtga tgcaagagcg tcgcgagcag cagcgcgggg ttcgtcgcga catccgggac
     4621 gagcacgcga tcgcgcatgc gccgcgcgcc gtcgagcaac cccatcacgt cgagcccggc
     4681 gagcgcagcg ggcaagaggc cgactgcaga cagcacgctg aaccgaccgc cgacgttctg
     4741 cggaatgtcg aacaccggat agccgcgctc ggctgcgacg gcacgcagcg cgcccttctc
     4801 cggatcggtc gtgcacacga tgtgtttctt cgcgcgctcg gggccgagac gcttgtcaag
     4861 ctgatccgcg acgacgagga attgcgcgca cgtctcggcg gtcgcgcctg acttcgtgac
     4921 cacgttccac agcacgcgat cgagcggcag gcgctcgatg atcgcggcga acgtgatcgg
     4981 atcgatgttg tcggggaaga acagccgccg tggcatgccg gcgctgcgtg tcgcgaggtg
     5041 atcgtacggc tgcgcgagcg ccgaatagag cgcgcgtggg ccgaggctcg agccgccgat
     5101 gccgagcgtg acgaccgtgt cgatgaacgg atcgagcgtg cgcgcgaagt ccatgcatgc
     5161 gcgcgcggtc gtgcggtcgt ccggcagatc gagaaaaccg atctcgccgc gctcccgacg
     5221 cccgagcgtg gtctcgaccg cgcgtctagc agaatctcct acggccgaga tcgcatgctc
     5281 gtcgggcccc ttgtcaccaa tccccgccga cgtcagcgcg gttgcatcga gaaccagctg
     5341 attgctcgac atccggggcg aaccctactg caaagttctt gtgcttgcga gcagatgcgg
     5401 ctacatccaa gtatcgatgc gacgcgctgt cctgctcgcc ggaggtgccc tgctcgcagg
     5461 cgcgggtctg ttcattcgct ctggcgcagg gccgacaccg aaggcgtcga tcgacggcga
     5521 accgatcgca acagcgccca agccgatcga agctgacagc aaagcgactc ccgaagagat
     5581 cgcgacgtac gaatctctcg gagccgatcc gatcaagcag gcgagcgtcg acctgcagct
     5641 cgacaagatg gagctgaagg acggccacta cgaagcgccg ctcaaggacg gccgccgcgc
     5701 gacgctcaca ctcgatccgg atctgcagaa gctcgccgag aagctgctca acgaatcgcg
     5761 cgcgccgcgc ggtgcgatcg tcgcgatgac gccggacggc aagatcctcg cgttcgccgg
     5821 ccgccgcacc caagagaaca agggcggcaa agaaggcacg ttcgactatc acctcgcgac
     5881 ggacgtgtgg gcaccggccg catcggtgtt caagctcgtc accgcgagcg cactgctgag
     5941 caacggcttc aaccccgacg acaaggtctg ctaccacggc ggcgttcgtt cggtgatgga
     6001 gtcgaacctc accgacagca agagcgacgg caactgcgag acgctcggct acggcgttgc
     6061 gcactcgaat aacgcgatcc tcggcaagct cgcgtatcag aagctcgacc cgacgaagct
     6121 cgatcatcaa gcgcgcgatc tcggctggac gttcgcgcca tcgttcggcg tcaaagcgac
     6181 gtgcggcgag ctcgacctgc cgaaggacaa gaatctcgac ttcgcgaaag cagcggctgg
     6241 cttcaacggc tcgcggctct cggtgctcgg cggcgccctg ctctccgcga tcttcgcgag
     6301 cggcggtcag cagcccgtgc cgcgcatcat cgaatcgatc aacggcaagc cgttcgccgg
     6361 gcagaaggcg aagcgcgtgc ttcccgaaac cgtcgcgaag accgtcgcga cgatgatgca
     6421 ggagacctgc gagtcgggca gcgcggcgaa gacgttccgc aagcgctcgt acgaggtggc
     6481 tggcaaaacc ggcacgctat cgacgaacaa gccgttcttc atggagcact cgtggttcgt
     6541 cgggtttgcc cccaccgacc agccgcaaat cgtgatttcc gttctgctcg gcaacccgga
     6601 gagctggcat ttgcgcggcc acgaagcggc caagcgcatg atcgatcgtg ccattcggcg
     6661 tgcgggagat cgcgaagaga acgtcgagac caaatcgact aaatcgagcg cccgcgcgtc
     6721 caagcgctaa cggtgatacg atcttgccgt cgtcctggtc ctgggaggaa ctatgtctag
     6781 gctcactttt ggcctcgtgc tcgcgtcgtc gctgctcggc ggcgttgcgc tagcggagag
     6841 ccaagccgag attgccgcgc ggctgaacga agaaggcaaa cagctcctgt tcgcggagaa
     6901 gtacgcggaa gcgagcgcga agttccagga cgcgcacagc cgcgtgccgg agccgaagta
     6961 cttctacaac cactgcatgt cgctgaccgc cgaaggcaag ttcggcgaag cgctcacggc
     7021 gtgcaacgcg gccgacaaga acgccgacgc atcgctcaag gacaagatcg ccaagctcga
     7081 gacgcgcatc cacgaggaag cgaagaagca aggcatcgat ctgcagccgg tcggtggtgg
     7141 tggcggtccc ggtgacacgc cgcccgtcga tccgaacaat ccgaaccaac cgcccgtcga
     7201 tccgaacaat ccaaacaacc cgccgcccgg tggtcagccg ccggcgggtc cggggatggc
     7261 ggtcggtcgt cccgtcggca acgtgctgtt ccaagcggcc ccggagaaca agtacacctg
     7321 gagcctcggc ttcgatctgt acggtggctc gggccaggtc ggccgctcgg gatacttcgg
     7381 caaggcgacc ggtggcattc gcgtcaaggg cgactacctg ttcaatcccg cgaagcggat
     7441 cggaacgcag gcgtattttc agctcacgca tttcggcgag ggcgacgaca tggcgagcac
     7501 gggcgcgctg tcgctcgacg tgttcgatct cggcatcgcc ggatacaagc acctgtgcgg
     7561 cccgcatgcg cgcggctgca tcacgccact catcggcgtg cagctggcgc tgatgagccc
     7621 ggcggaccag atggacgcgt cgaccggtga ccaggtcttc aactacgcat ccgtcggcgc
     7681 gcgcctagag ctcgcgcttt cgtacgcgct cggcagccac ttcgagcacg tcctcagcct
     7741 caccggcggt ctcaacgcgt acacgaaggt gttctcggag ccgagcgaag gcgatagcgc
     7801 agcgttctgg ggtctcgaca agggcggcgc ggccggctac atcggcatcg gctacacgta
     7861 ccgcttcaac acaccgttcg gcaccagtcc gttcgtagtg ctgtagtccg cgtcgctgtg
     7921 tgatgcacga gcccggcgct tcgttgggac gggcaacgat cattttgacg tcccgcgaga
     7981 cgcagtcttc gtgacgctcc actagaccga cccgtcacga attcgggtat ggtctccgcg
     8041 ctgcaaggta acgcagtgca ggagccaccc gtcatggccg agaaactcag cgcgaagctc
     8101 atcctccccg gcggcaaaga gaaccagcta cccgtgatcg tcggtaccga agacgagcac
     8161 gcggtcgata tccggaacct ccgccaggag accggctaca tcacgctcga tagcgggtac
     8221 atgaacacgg gctcgaccac gagcgcgatc acctacctcg atggtgaaca aggcatcctg
     8281 cgctaccgcg gttatccgat cgaagagctc gccgagcact cggacttcgt cgagaccagc
     8341 tacctcctga tcaacggcga gctgccgaac cgcgccgagc gcgaggcatt ccgcaccaag
     8401 ctcacgcggc acagcctgat ccacgaggac atgcaccact tcttcgcggg cttcccgccg
     8461 accagccatc cgatggccgt gctgtcggcg atggtgacgt cgctgtcggc gtactacccc
     8521 gactcgctcg agcgcaatgc gccgtccgat ctgcacatca cgcggatctt gtcgaaggtc
     8581 cgcacgatcg cggcgttcgc ctacaagaag agtatcggcc agccgatcgt ctacccgaag
     8641 aacgcgctgc gctactgcgc gaacttcctc cacatgatgt tcgccgtgcc gagcgaagac
     8701 tacgaagtct cgccggtgct cgagaaggcg ctcaaccagc tcctcatcct gcacgccgat
     8761 cacgagcaga actgctcgac gtcgacggtt cgcatggtcg gcagctcggg cgcgaacctc
     8821 tacgcggcga tcgccgccgg catcctcgcg ctgtggggcc cgctccacgg cggcgcgaac
     8881 caggccgtca tcgagatgct cgaggagatc aaagcctcgg gcggcgatca caagaagttc
     8941 atcgacgacg tgaagaacaa gcgcgagaac cggcgcctga tgggcttcgg tcaccgcgtc
     9001 tacaagaact acgatccgcg cgcgaagatc ctcaagaaga tgtgcgacga ggtgctggtg
     9061 cacctcggca tcaaggatcc gctgctcgac atcgcgaaga acatggaaga gatcgccctc
     9121 aaggacgatt acttcgtgtc gaagaagctc tacccgaacg tcgacttcta cagcggcatc
     9181 atctaccgcg cgatgggcat cccgacgaac atgttcaccg tgatgttcgc gctcggccgc
     9241 ctgcccggct ggatcgcgca ctggaaagag atggtcgagg atccgcagac gaagatcggt
     9301 cgtccgcgcc agatctacac cggcccgaag cagcgcaagt acgtcccgct cgagaaccgc
     9361 gcctaatgag gcggtccgcg gtcgtcgtcg cgctggtcgt gatggcgagc gcatgcggca
     9421 agaacgagtc gtcgccagcg gcgagcggat cggctgcggg ttccgccgcg gcgaacgatc
     9481 cgtggtcgaa gccggctgcg cccgcccctg ccccagccgc tgctccctgc gacgacgccg
     9541 atatcaagaa gcacatcgac gacagcctcg cggtgtcgct cgcgtacatg gcggcgctcg
     9601 agaagaaagc gctgaagtgg aagacggact gctcggtcgc gaagaaggat ctgctcgcgc
     9661 tcgagcccga agccacgaag ttcatggcct cggtgaacga cttcatgacg tggggcaaga
     9721 cgctgacgcc cgagtgcggc aagcgggtcg ctgaaatcgg cgagcagtac gacgccgcgc
     9781 gcgacatcga aaagcgtacg cccgagctag aagcacacgt gaagacgatc ctcgagcact
     9841 gcaaagacta cccgggcttt caggacgccg cggccaaagg cctgcgcgtc atgcacaaga
     9901 agaagccgta actacttctt gcagctcgcg ggcaacactt cctgccggta ctggccttcc
     9961 cagttgccgg ctggatcgta ggtgcacacg tagatgtcgt tgccgttgca ctccacgtgg
    10021 ccgcaaccaa cctgttgcgt gctgcgccag acgacctgtg tgaagtggcc cgtccgcgcc
    10081 gagaagccgc cgttcggaaa cttgtagagc ttgatctcgt cgtaccacat ggcaacggtg
    10141 ctcgtcggat cgagcgcgcc gacggtgccc gccgcgaggt tctcgccgta ctgcgcgccc
    10201 gggctgtggc cgaacacgca gcccttgctc ttgagcgtgt tcgcccagtt ctgcgcgatg
    10261 tcggcgagct tcttcgacca cgtcagtgga ccggcgcagt gcttcgcacg cacttcgttg
    10321 tgcgcgtcga tgaactgctg cgcgaacggg tcgagatgct ggggcttcgg cggctcgggc
    10381 ttcggcggct cggacttcgg cgcgcttgtc ggctttccac cctttgctgg cgccggtcgc
    10441 gcggcagcag tttgaccgtg ggccgtccgc gtatatggcg gcggatagtc ggagtacttg
    10501 ggcttgcacg cgaccagcgc catcgcgagc accgcgatga tcgtcgcggg cgaatgagtg
    10561 aacgacctca tgcggtgatc atgacacgcg cttcttcgcg gcgcgtttgg tggtcttcgc
    10621 agcagcggtg cgcttcgtca ccggtttacg tttgttcaca gtcgcgggcg cagccgcacc
    10681 accttcgagc tgcttgagaa acttcttcgg cgcttgtgcg cgatagctct cgtcgagcca
    10741 ttccttgagc atctcgacgg gaagcgcttt gccttcggga aacttggccg agacccaacc
    10801 cgccttgccg aggccgtagg ccgccgggct gcagaacggc agcatcagcg cgaccgcagc
    10861 cgactgcggc agcttgcacg agatgccgaa cggctcgcct tcgttcgaca ggtacgcgaa
    10921 cgtcttgtcg ttgaccgcga ggtccatgtg cccgggccat gggctcttga agtgcgcacc
    10981 ggggtactgc attccgaacg cacgaagctc ctgcagcacc ttgtcggtgg ccagggcctt
    11041 cttcttcgcc atggaccgag tgtatggtgc gcgcgtgcct ggaccaatgg ttgcggagtc
    11101 aacatcgcaa gcgctgacgc aaggcgtccg ggtccgcgtc cagtcgatgt acctgcccga
    11161 ccaatcgtcg ccgaacgacg atcgctacgt gttcgcgtac acgatcacga tctcgaacga
    11221 gagcacgttt accgcgcagc tgcgcacgcg gcattggatc atcacggatg gtcgcggtgg
    11281 cgtcgaagag gtcaaaggcg atggcgtggt cggcgagcaa ccgcgcctgt caccggggca
    11341 gagcttccag tacacgtcgg gctgcgtgct gacgacgccg atcggaacga tgcacggcac
    11401 ctatcggttc tggcgcgacg acggcaccta cttcgacgcc gtgatcgctc cgttctcgtt
    11461 ggcatcgccc gtgcgcgacc aagaagcgaa ctagaacggc tagttggctc gagtgcgggg
    11521 atgtgggcag gaaaaacgca aagccttcac gtaggctatg cgtctaacga ccagatgacg
    11581 aatgctgagc gcgaggcagc gaccgcgacc gcactggtcg cggcggcgaa ggcgggggac
    11641 acgcgagcat ttgaagcgct cgtgaagcgc tatcgcaagc gcatctacgc actcgcgctg
    11701 cacatgacgc ggtcggcgag cgaagccgac gacatcacgc aagaggtctt cctcaaggcg
    11761 tatcgcgcgc tcggtcagtt cgaaggtcgc tcgcagttct tcacctgggt ctatcggatg
    11821 accgtgaatc gttcgctcaa cgccaagcgc gatcgtgcgc ggcgcggcga ggacacgatc
    11881 gatgaccctc gtctcgagct cgccgtcgcg gtcgacgcgc gcggcaatcc ggggcgcgaa
    11941 gccgagctac gccagagcta tgcacggctg cttcgcgcgc tcgatcagct accgatcgac
    12001 atgcgcacga cggtgattct cgtcagcctg cagggcatgt cgcacgccga ggtcggcatc
    12061 attcaaaagg tgtcggacgg cacgatcgcg tggcgcatgc acgaagcgcg gcgccgcctg
    12121 cacgacgcga tggcacccga gaaggtgacg cgcaaacgcg gtgagctctc ggccgagctg
    12181 tcgcgcgcgc tgtccgagta cggcttgctg gtgcccggca aagcgcgcgc ttaggtcatc
    12241 aggtcgtacg ggccaccgcg ctcgacggct ttcttgaacg ccggccgctc gtgcatgcgc
    12301 gtgacccacg ctttgatcgc gtcgggaacg tcctttacgc gcaaggcgag cgcttccatc
    12361 gggaagctca tctggatgtc ggccgccgtg atctcgccgc ccgcgaagta gtcgcgggtg
    12421 ccgagctcgc tcgcgagaaa cgcgacgtgg cgatcgaggt tcggcgcgac gtagctgccc
    12481 tcgagcttgc ctgcgatcgc cttcgcgatc ggccgcacga ggaacggcgt gcgcttgagc
    12541 ttgctcgtga tgaggcgcag cagcagaaac ggcatcagcg agccttcggc gtagtgcatg
    12601 aagtagcgat agcgccggtg gttcggggtg tccttcggcg gctgcatctt gccgttgccg
    12661 tatttctcga cgatgtattc gaggatcgcg gcggactccg cgatcgtcac atcgccgtcg
    12721 gtgatgacgg gtgatttgcc gagtggatgg atcttgtcga gctccggcgg ggcgaggtcg
    12781 gtcttcggat cgcgttcgta cttcacgatc tcgtacggca caccgagctc ttccagcagc
    12841 cagagcacgc gctgcgaacg cgagccgttg aggtgatgga cggtgagcat cgcgggcgaa
    12901 attaacttag tatccgcgcc atgcgactcc ttctcgccac actcctgctt gccggctgtg
    12961 cgcagctgat gccggcggcg aaacaagagc cgttcccggt catggaagtg aaagcgacac
    13021 ggccgccgcc cggtccggcg cgagtcgtgc tcactgaatc gaacatcgcg atcatggaca
    13081 aggtccagtt cgagaccggt aaggcgaacc tgctgccagc gtcgaacgcg ctgctcgacg
    13141 aggtcgcgaa gatgctcaag gacaacccgc aggtcgagct gatctccgtc gagggtcata
    13201 ccgattcgac ggggtcgccc gacttcaacc gcaagctgtc gcaacagcgc gcggagtcgg
    13261 tcgcgaagta tctctccggc aaaggcgtga aggccgcgcg catggagccg aagggcttcg
    13321 ggcccgaccg gccgatcgcc gacaacgaga ccgatcaagg acgcgaagcg aatcgccgcg
    13381 tcgagttcaa catcctcaag cagggtccga agaaagtgct ggtgaaggat gaataagctc
    13441 actctattca tcgctgccgt gtcgctctcc gccctcgccg gcgcctgcgc gaagcagggc
    13501 atgacgcccg cggtgcgcaa cgagatcacc gcgaagctgc agtcgacgca gacgccgatc
    13561 cagcagtgct acgcgcgcca gctcgcaacc aatcgcaagc tggccggcat ggtcgtcgcg
    13621 caagccgcaa ttgcgcccga cggccagttc ggcgaggtct cgctccgccg cgacgagccc
    13681 gccgatccgg tgctgcgctt ctgcgtcgtg caagagatcg cgaagctcaa gctcgacaag
    13741 ccaaccggcg aacgcgtggt gctcgacagc attccgatca agttcgtgtg ggcgaacccg
    13801 tgagcaaaac gccgacacgt agcccgaccc acgaagacgt cgatccgacg cgactcgcgg
    13861 gcatgggtct cgagatcgag gacatcgcgg acggcgacac ggtcgcgctc cctgccctcg
    13921 tcgagctcgc cgaagaccgc ggccgcccag tcagtcatta tctggctgcg attccgctcg
    13981 cgaccgagct gcggctcgcc ggcaccgggc cgctcacgct caagatctgc gccggcacct
    14041 gccagcgctg gggcgcgctc gacttgctcg accacgtcgc cgagcgggtg accaagggtg
    14101 ccaagctgac gctcgccccc gtgagttgcc tggacaggtg cgatcaggcg cctgcctgcg
    14161 agatccatgg cccgcacggc cagctggtcg tagccccggc taccgccgct acgctcgacg
    14221 aggccatcga ctcgttatcg taacgttttg tcgcatgtgt cgcagtctgt cgcgcactgg
    14281 cgcgacggcg atgcgagcgc tagcctgggt ctcgtcgtgt tgcgcctgaa caaggtcaac
    14341 aagtactacc aggtgggcac gcacagcctt cacgtgctgc gtgatgtcga gctcgaggtc
    14401 gccgacggcg agttcgtcgc gatcatgggc gcgtcgggca gcggcaagtc gacgctgctc
    14461 aacatcctgg gcatcctcga cgactacgac tccggcgagt actggctcgg cgacacgctg
    14521 atccgcaagc tgtcggagac gcgcgccgcc gagtaccgca accgcttcat cggcttcgta
    14581 ttccagtcgt tcaacctgct gccgttcaag acggcgctcg agaacgtcgc gctgccgctc
    14641 tattaccagg gcgtcacccg ccgcaagcgc aaccagatcg cgatgcagta tctcgagcgc
    14701 gtcgggctgc gcgactgggc cgaccacgtg ccgtcggaga tgtcgggcgg tcagaagcaa
    14761 cgcgtcgcga tcgcgcgctc gctgatcggc aagccgcgcc tgatcctcgc cgacgagccg
    14821 accggcgccc tcgatagcga gaccagccgt cagatcatgg atctgatgag cgagatcaat
    14881 tcgaccggca tcaccgtgct cgtcgtgacg cacgatccgg aagtcgcgag ccgcacgcaa
    14941 cggacgatcc gcctgcgcga cggcaaggtg ctgagtgcgc acgaagcagc gctgtcgaat
    15001 cccggcatgc cggcggcgcg cgactcgatt cccccgtcgg cggggacgta gatggcgatc
    15061 gcggcgctca agctgctcgg aatcctcgcc gggcttgcgc tcgcggtcac cctcatcgtc
    15121 gtcgtcgtaa tcgtgctgat cgcgatcgtg cgaatggcga tcgtgggcgt cgtccagtgg
    15181 cgcgatttgt tctcgctcga tcgctggagc gaggtcctga tcacgatcgg tcgcaacaag
    15241 ctacgcaccg cactcacgac gatcagcgtc gcgtggggca tcttcgtgct cgtcatcttg
    15301 ttgggcctcg gcaacgggct caacaatggt gcgcaccacg agttcgctcg cgacgcgacg
    15361 aacggggtct ggatcaccgg caacaagacc tcggaggcgt tcggcggcta cgacatcggc
    15421 cgccgcatca aattcgacaa ccgcgactac gac
//
LOCUS       DASUFX010000362         8500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1863218, whole genome shotgun sequence.
ACCESSION   DASUFX010000362 DASUFX010000000
VERSION     DASUFX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863218"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFV99_14400"
     CDS             complement(<1..263)
                     /locus_tag="VFV99_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5060554.1"
                     /translation="MADHLFLRACRRQPVPRTPIWMMRQAGRYLPAYRAVRSRASFLE
                     MCRTPELACEVTLQPIDEYGFDAAILFSDILITLPAMGLDVAFP"
     gene            305..1345
                     /locus_tag="VFV99_14405"
     CDS             305..1345
                     /locus_tag="VFV99_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060555.1"
                     /translation="MSAEAIAIVVAPEGATGPLEGIVERLTSTNIVVHVVEDVPSAAL
                     IASRTPSEPPCVLLDLRDLSSGSELEDVKQACERVRKAAHAIQHTMPVAVTSSANAGA
                     VVALVRAGAGDVIDLQLEGIGSARAVVVRVYHRQVELSHQVYTARALRSMIEDMLKDL
                     IRTERRSIDLEEKLAQFESVTGETGALSDQREPAILLVEQDLAVAREIAERLEAEGLS
                     TFAYTSGEEAARELDTLLAGGTGFDLALIAAQLPGIDGLETVRRLRDQLPGLPAFLMT
                     AVHDADLAARAADLGVVGFVQKPLPDIDEVVARLSQLAREALDRTREQLYLQRIKARH
                     ERVLARYRSLPR"
     gene            1379..2668
                     /locus_tag="VFV99_14410"
     CDS             1379..2668
                     /locus_tag="VFV99_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5060556.1"
                     /translation="MAGAVLSIKLRCASWQQLATIYKRDLSRGSMFLKATTQPAIGTL
                     VRIDLELPSASVIELQGTIVKHVTDPQRGTGVDLKLDPLPTKSVWMIESALEAAGPKG
                     RASTGNEANQPRERASTVTDGEEVTAAEAELIRALEAEAESLRKLNPFLVLGVGYETT
                     DEDVRHAFGELTKRYHPDRFARYQSAKLRQVAAEIFILIRDAYRRLADPKAREQVVAT
                     LRRAPQRAQTEPRGSASSSVSPPQQRGVTRVAVPTKPPEKAVKPASPHDAPTNPSVRP
                     AELAHTDLAQNELAVPIAKPVRGFDPTVPLPPQPAVPEKPKRAPTPPPEPVDTEAIEE
                     LIEVGKYDEALKEYKALFKKNPHDRGLRAGIELCEGLKALAARDRLEAAQYFEAALEL
                     DPSNERAARELAEMRRQATNERKGLLSRLMGKKEPPP"
     gene            2665..4326
                     /locus_tag="VFV99_14415"
     CDS             2665..4326
                     /locus_tag="VFV99_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5060557.1"
                     /translation="MSRIIGIDLGTTNSCVAVLDDKGQPKTLTAADNERTTPSWVAWA
                     PDNTISVGTRARRQAVTNASATVYGAKRLIGRKVNAEDVAWFAKLAPFRIVAAPNGDA
                     WVRVHGQPISPQEVAAHVLRRLRQVAEEALGEPVTRCVVTVPAYFDDAQRQATRDAGQ
                     IAGLDVIRILNEPTAAALAYGAHRVREGRRIIAVFDLGGGTFDISIMSVENGIFEVLA
                     TGGDSALGGEDWDRRVLERIVDEVFDSHRVDLTGVPMALSRLREAAENAKKALSSANE
                     ALIQLPFLANDQHGAPINYERTITRDEVEQLTQDLLDRVEHPCKRALYDAGLTAADVE
                     EVLLVGGMTRWPAVQRTVERIFNRKPSKGANPDEVVALGAASYASILGGDADDAALLD
                     VTPHDLGIKVGDSGFSVVIPRNSMLPVRARKLFATTSEDQKFVSIELYQGESNDLRGN
                     RKLGQVVLDELPPGPAGSVRVELVMTVDVESILGVTARELRSGKEASVTIRPSGGLSQ
                     REIVEIINRRRQESSFTAKLPSEGGTVARVTTREQAAVTPIDDEK"
     gene            4326..4856
                     /locus_tag="VFV99_14420"
     CDS             4326..4856
                     /locus_tag="VFV99_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5060558.1"
                     /translation="MSAKERVDPAVLKVADAVGALIEGWGFKRNMGRMWAVLYLEDHP
                     LSAAELGDRLGLSSGAVSMLLAELLQWGIVKKAWVVGERREFFEPETSIWKMVSRVFR
                     ERELNWIKTASDEFEAAGKELGGVRDARRKLIAERIASLTQLAQVGATLLEAVLEGES
                     VDALPLKTIGDLAKKS"
     gene            4866..5867
                     /locus_tag="VFV99_14425"
     CDS             4866..5867
                     /locus_tag="VFV99_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5060559.1"
                     /translation="MIRARELTKHFKVHKRPPGFANALKSLVRRKYEVVKAVDGVSFT
                     IERGERVGFLGPNGAGKTTTLKVLSGLLHPTSGDVDVAGHVPFKREAALLGKITLVMG
                     QKSQLIWDLPPAETYAMNRAIFDVDQSEFDKTLGELTELLELGDLVNKPARQLSLGER
                     MKCELAAALLHRPTTLFLDEPTIGLDVAMQVAIRDFVRSYNQRHDATVLLTSHYMDDV
                     VALCPRIIVIDQGKLIHDGDLRQLIKTMDPDKRVSFTLVGPAVADEEIAKLGTVLSRE
                     AQRTTLRVSEKELPNVVGHLLGTLKAADLAIEDPPLEDVLRVMFGKSKNNNHGNGAS"
     gene            5864..6682
                     /locus_tag="VFV99_14430"
     CDS             5864..6682
                     /locus_tag="VFV99_14430"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5060560.1"
                     /translation="MRSAPARALRALPTLLRIGVAETVAYRAEFVVWMLTSTMPLIML
                     SLWTSVASEGPFQQYSSADFVAYFLGVLIVRNLTGNWVAFQISEEVRMGTLSMRLLRP
                     IHPFFAYATSHAAALPFRSLIALPIAIILLISSGASSLTTEPAQLALLLPSICLSWLI
                     TFGLMFAIGCCAFWITQAFGIFNFYFGLWSLMSGYLMPMDIMRSKFPIVADVAVRLPF
                     YYMLGAPVELMTKHLSSSQLVELLGWQIAWGFVSVLIALYVWGKGVRHFEAVGG"
     gene            6679..7467
                     /locus_tag="VFV99_14435"
     CDS             6679..7467
                     /locus_tag="VFV99_14435"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5060561.1"
                     /translation="MNRYVTLFTTQLRTSVATAMQYRANFVIEIVLSVAWLAIALLPL
                     MVLFEQRDNVAGWDRPSALIVMAYFMAVKAIMEGMVTPSLVSLVEKIRTGAFDYVLLK
                     PVDAQFMVSTGGYEPQKAIDLIGAAVLSCYAFSQRGVWPNGEQVGIGILLLIAGVFAM
                     YSLWIICAAASFWVVRLDNLIYLLMAIFDTARWPIQVFRGVWRFLFTFIIPVAIMTTF
                     PAMALLGRLSYRTTLFTLAGALGMVFVSRMIWRAAIRNYTSASS"
     gene            complement(7472..>8500)
                     /locus_tag="VFV99_14440"
     CDS             complement(7472..>8500)
                     /locus_tag="VFV99_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5060562.1"
                     /translation="FESPITHQLAFLGTNKERTNQIVATISQHQPRALAWDSAHDALY
                     VAGLGSDSIIQLKNASQVGIAEGVSASLTKGKDRCGPDGIAVGQDGNLFIWCSFTRSV
                     TTLEVIDGKGKLATAAKIDAGESLVASSLNEKQHEGLVLFHSSQPQISARGSLACASC
                     HPDGRADGLSWRIDKHELQTPLLAGRIVDTHPYKWDGGDADLPASLTGTMKRLGGFGI
                     TKEQTGALASYLETLQKPTVPTHDAQQIARGEKLFDSAEVGCRTCHDGKQFTDQDKHK
                     FQAATLPEADTPSLIGVAASAPYYHDGSAATLEALLRDRGNVHGMAETAKLSDSQVAD
                     LIAFLESL"
BASE COUNT         1470 a         2791 c         2823 g         1416 t
ORIGIN      
        1 gggaacgcga cgtcgaggcc catcgccggc aacgtgatca ggatgtcgga gaacaggatc
       61 gccgcgtcga acccgtactc gtcgatcggc tgcagcgtga cttcgcacgc gagctcgggc
      121 gttcgacaca tctcgagaaa cgacgcgcgc gatcgcacgg cgcgatacgc gggcaagtaa
      181 cggcccgctt gccgcatcat ccagatcggc gtgcggggaa cgggctgtcg tcggcacgcg
      241 cgcaggaaca agtgatcggc catcgcgcac atcctagtga tatcgtgcgc gccagacaaa
      301 ccacatgagc gccgaagcca tcgcgatcgt ggttgcaccg gagggcgcta ccgggcccct
      361 ggaaggcatc gtcgaacggc tcacgagcac caacatcgtg gttcacgttg tcgaagacgt
      421 accctctgcc gcgctgatcg cgagccgcac cccctccgag ccaccgtgcg tgctgctcga
      481 tttgcgcgac ctcagcagcg ggagcgagct cgaggacgtg aagcaggcct gcgagcgcgt
      541 acgtaaagcc gcgcacgcga tccagcacac gatgccggtc gcggtcacga gctcggcaaa
      601 cgccggtgcc gtggtcgcgc tggtccgcgc gggtgccggc gacgtgatcg atctgcagct
      661 cgaaggcatt ggctcggcgc gcgcggtcgt ggtccgcgtc tatcaccgcc aggtcgagct
      721 ctcgcatcag gtgtacacgg cgcgcgcgtt gcgctcgatg atcgaggaca tgctgaagga
      781 tctgatccgg accgaacggc gctcgatcga cctcgaagag aagctcgccc agttcgagag
      841 cgtcaccggc gagaccggcg cgctcagcga tcagcgggag ccagcgatct tgctcgtcga
      901 gcaagacctc gcggtcgcgc gcgagattgc cgagcgcctc gaagcggaag gcctatcgac
      961 gttcgcgtac acgagcggcg aagaagctgc gcgcgagctc gatacgctgc ttgcgggcgg
     1021 caccggcttc gacctcgccc tcatcgccgc ccagctcccc ggaatcgacg gcctggagac
     1081 cgtgcgaaga ctccgcgacc agcttccggg gctaccggcg tttctgatga ccgcggtcca
     1141 cgatgccgac ctggcggcac gggcggccga cctcggtgta gttggattcg tacagaagcc
     1201 gctccccgat atcgacgagg tagtcgcgcg actgtcccaa cttgcgcgcg aggcgctcga
     1261 tcgcacccgc gagcagctct atctccagcg gatcaaagcg cgccacgaac gtgtgctcgc
     1321 gcggtaccgc tcgttgcctc ggtagcgtaa aatcgcctat gatttcgcgg tttcactgat
     1381 ggcgggagcg gtcctcagca tcaagcttcg ttgcgcctcc tggcagcagt tggcgacgat
     1441 ctacaagcgc gatctgagtc gtggctcgat gttcctcaag gcgacgacgc aacctgcgat
     1501 cggcacactc gtccgcatcg atctcgagtt gccgagcgcg agcgtgatcg agctgcaagg
     1561 aacgatcgtc aagcacgtca ccgatccgca gcgcggcacc ggcgtcgatc tcaagctcga
     1621 tcctctgccc acgaagtcgg tgtggatgat cgaaagtgca ctcgaggctg cgggaccgaa
     1681 gggccgcgct tcgaccggca acgaagcaaa ccagccgcgc gagcgtgcga gcacggtgac
     1741 cgacggcgaa gaggtcaccg ctgcggaggc cgagctgatc cgtgcgctcg aagcggaagc
     1801 cgagtcgctg cgcaagctga atccgttcct cgtgctcggc gtcgggtacg aaaccaccga
     1861 cgaggacgtg cgccacgcgt tcggtgagct gacgaagcgc tatcaccccg atcgcttcgc
     1921 acgctatcag tcggcgaagc tgcgccaggt cgccgccgag atctttattc tgattcgcga
     1981 cgcgtatcgc cggctcgctg atcccaaggc tcgcgaacaa gtagtcgcga cactgcggcg
     2041 agccccgcaa cgtgcgcaga ccgagccgcg cggctcggcc tcgagctcgg tctcgccacc
     2101 gcagcaacgg ggtgtcacgc gcgtcgccgt acccacgaag ccgccagaga aggccgtcaa
     2161 gccggcgagc ccgcacgacg cgccgacgaa tccgagcgtg aggccggccg agctcgcaca
     2221 cacggacctc gcgcagaacg agctcgctgt gccgatcgcc aagcccgtcc gcggtttcga
     2281 cccgaccgtg ccgctgccac cgcaacctgc ggtgcccgag aagccgaagc gcgcgcccac
     2341 accgccgcca gagcccgtcg ataccgaagc gatcgaggag ctcatcgagg tcggcaagta
     2401 cgacgaggca ctcaaggagt acaaagcgct gttcaagaag aacccgcacg atcgcggact
     2461 gcgtgcaggc atcgagctgt gcgaaggcct caaagcgctc gctgcgcgcg accgactcga
     2521 agcggcgcag tacttcgagg ccgcgctcga gctcgatccg tcgaacgaac gcgccgcgcg
     2581 cgagctcgca gaaatgcgcc gccaggccac gaacgagcga aaggggctgc tgtcacgcct
     2641 catgggcaag aaggagcctc cgccatgagc cggatcatcg ggatcgatct cggaacgacc
     2701 aactcgtgtg tcgcggtgct cgacgacaag ggccaaccca agacgttaac ggcggccgac
     2761 aacgaacgga cgacaccgtc gtgggtcgcg tgggctccgg acaacaccat tagcgtcggc
     2821 acccgtgcgc gccggcaagc ggtgacgaac gcctcggcaa cggtctatgg cgcgaagcga
     2881 ttgatcggcc gcaaggtcaa cgccgaggac gtcgcgtggt tcgcgaagct cgcgccgttc
     2941 cgcatcgtgg cagcaccgaa cggcgacgca tgggtgcgcg tgcatggcca gccgatcagt
     3001 ccgcaagagg tagcggccca cgtgctgcgc cggctgcgcc aggttgccga agaagcgctc
     3061 ggcgagccgg tcactcgttg tgtcgtcacc gtgccagcgt acttcgacga tgcgcaacgc
     3121 caggcgacgc gcgatgccgg tcagatcgcg ggtctcgacg tcatccgcat cctcaacgag
     3181 cccaccgctg cggcactcgc ctatggcgcg caccgcgttc gcgaaggccg ccggatcatc
     3241 gcggtgttcg acctcggggg tggcacgttc gacatctcga tcatgtccgt cgagaacggc
     3301 atcttcgagg tcctcgcgac cggcggcgac agcgcgctcg gcggcgaaga ctgggatcgc
     3361 cgcgtgctcg agcgaatcgt cgacgaagtg ttcgatagcc atcgcgtcga tctcaccggc
     3421 gtgccgatgg cgttgtcgcg gctgcgcgaa gcagccgaga acgcgaagaa ggcgctgtcg
     3481 tcggcgaacg aggcgctgat ccagctgccg ttcctcgcga acgatcagca cggcgctccg
     3541 atcaattacg agcgcacgat cacgcgcgac gaggtcgagc aactcacgca agacctgctc
     3601 gatcgcgtcg agcatccgtg caagcgcgct ctttacgacg ccgggctcac cgccgccgac
     3661 gtggaagaag tgctgctcgt cggtggcatg acgcgctggc cggcggtgca gcgcaccgtc
     3721 gagcgcattt ttaaccgcaa gccgagcaag ggtgcgaacc ccgacgaggt cgtcgcgctc
     3781 ggcgccgctt cgtacgcgag catcctcggc ggcgacgccg acgacgcggc actgctcgac
     3841 gtcacgccac acgacctcgg catcaaggtc ggggacagcg ggttctcggt cgtcattccg
     3901 cgcaactcga tgctgccggt acgtgcgcga aagctgttcg cgacgacgtc ggaggaccag
     3961 aagttcgtat cgatcgagct gtatcagggc gagtcgaacg acctgcgcgg caaccgcaag
     4021 ctcggtcagg tcgtgctcga cgagctgccg cccggccctg ctggctccgt acgcgtcgag
     4081 ctcgtgatga ccgtagatgt cgaatcgatc ctcggagtca ccgcacgcga gctccgcagc
     4141 ggcaaagaag cgagcgtgac gattcgcccg tccggtggtc tgtcgcagcg cgagatcgtc
     4201 gagatcatca accgccgtcg ccaggagagc tcgttcaccg cgaagctgcc gagcgaaggc
     4261 ggcaccgtcg cgcgcgtcac gactcgcgag caggccgccg tcacgccaat cgacgacgag
     4321 aagtaatgtc tgcaaaagaa cgcgtcgatc cggcagtcct caaagtcgct gacgcggtcg
     4381 gtgcgctgat cgagggttgg ggcttcaagc gcaacatggg caggatgtgg gcggtgctgt
     4441 atctcgagga tcatccgctc agcgcggcgg agctcggtga tcggctcggc ttgtcgagcg
     4501 gcgcggtgtc gatgctgctc gccgagctat tgcagtgggg catcgtgaag aaggcctggg
     4561 tcgtcggcga gcgccgcgag ttcttcgagc ccgagacgtc gatctggaag atggtgtcgc
     4621 gcgtgttccg cgagcgcgag ctcaactgga tcaagaccgc gtcggacgag ttcgaggccg
     4681 ccggcaaaga gctgggaggc gtccgagacg cacggcgcaa gctcatcgcg gagcggatcg
     4741 cgagcctgac gcaactcgcg caggtcggcg cgacattgct cgaggccgtg ctagaaggcg
     4801 aatcggtgga cgccctgccg ctcaagacga tcggcgacct tgcaaagaag agttgaggcg
     4861 agctgatgat ccgcgcccgg gagctgacga agcacttcaa ggtgcacaag cgcccgcctg
     4921 gctttgccaa cgcgctgaag tcgctcgtgc gccgcaagta cgaggtcgtc aaagcggtcg
     4981 acggcgtcag cttcacgatc gagcgcgggg agcgcgtcgg cttcctcggc ccgaacggtg
     5041 ccggcaagac cacgacgctc aaggtgctgt ccggcctgct gcacccgacg agcggtgacg
     5101 tcgacgtcgc cggccacgta ccgttcaagc gcgaggccgc gctgctcggc aagatcacgc
     5161 tcgtgatggg gcagaagtcg cagctgatct gggacctacc gccggccgag acatacgcga
     5221 tgaaccgcgc gatcttcgac gtggatcagt ccgagttcga caagacgctc ggcgagctga
     5281 ccgagctgct cgagctcggc gacctcgtca acaagccagc gcgtcagctg tcgctgggcg
     5341 agcgcatgaa gtgcgagctc gcggccgccc tgctccaccg cccgacaacg ctgttcctcg
     5401 acgagccgac gatcggcctc gacgtcgcga tgcaggtcgc gattcgcgac ttcgtgcgca
     5461 gctacaacca gcgccatgac gcgacggtgc tgttgacgag ccactacatg gatgacgtcg
     5521 tcgcgctgtg cccgcgcatc atcgtcatcg atcagggcaa gctgatccac gacggtgacc
     5581 tgcgccagct gatcaagacg atggatcccg acaagcgcgt gtcgttcacg ctcgtcggcc
     5641 cggccgtcgc cgacgaagaa atcgcgaagc tcggcaccgt gctgtcacgc gaagcgcaac
     5701 gcacgacgct gcgcgtctcc gagaaagagc taccgaacgt cgtcggtcac ctgctcggca
     5761 cgctgaaggc cgctgacctc gcgatcgagg atccgccgct cgaggacgtg ctgcgcgtga
     5821 tgttcggcaa gtcgaagaac aacaaccacg gcaacggggc gtcgtgagat cggcaccggc
     5881 ccgcgcacta cgcgcgctgc cgacgctgct ccgcatcggc gtcgccgaga cggtcgcgta
     5941 ccgcgccgag ttcgtcgtct ggatgctgac gtcgacgatg ccgctgatca tgctcagcct
     6001 gtggaccagt gtcgcgagcg agggtccatt ccagcagtac tcgtcggcag acttcgtcgc
     6061 ctacttcctc ggtgtgctga tcgtgcgcaa cctgaccggc aattgggtcg cattccagat
     6121 ctcggaggag gtccgcatgg gcacgctgtc gatgcgcctc ttgcggccga tccatccgtt
     6181 cttcgcctac gcgacgagcc acgccgctgc actgccgttc cgatcgctga tcgcgctgcc
     6241 gatcgcgatc atcttgctca tcagctcggg cgcgagctcg ctgacaactg aaccggctca
     6301 gctcgcgctg ctgctgccgt cgatctgcct gtcgtggctg atcacatttg gcctcatgtt
     6361 cgcgatcggc tgctgcgcgt tctggatcac gcaggcgttc ggcatcttca acttctactt
     6421 cgggctgtgg tccctgatgt ccggctacct gatgccgatg gacatcatgc gatcgaagtt
     6481 cccgatcgtc gccgacgtcg ccgtgcggct gccgttctat tacatgctcg gcgcgccggt
     6541 cgagctgatg acgaagcacc tctcgtcgag ccagctggtc gagctgctcg gttggcagat
     6601 cgcgtggggc tttgtgtcgg tgctgatcgc gctctacgtc tggggcaagg gcgtgcgcca
     6661 cttcgaggcg gtgggcggat gaaccgctac gtcacgctgt tcacgacgca gttgcgcacg
     6721 agcgtggcga ccgcgatgca ataccgtgcg aacttcgtga tcgagatcgt gctctcggtg
     6781 gcgtggctcg cgattgcgct gttgccgctg atggtgctgt tcgagcagcg cgacaatgtc
     6841 gccggttggg accgcccgtc ggcgctgatc gtgatggcct acttcatggc ggtcaaagcg
     6901 atcatggagg gcatggtgac accgtcgctg gtatcactcg tcgagaagat ccgcaccggg
     6961 gcgttcgact acgtgctgct gaagccggtc gacgcgcagt tcatggtctc caccggcggc
     7021 tacgagccgc agaaggcgat cgatctgatc ggagccgccg tcctgtcgtg ctacgcgttc
     7081 tcgcagcgcg gtgtgtggcc gaatggcgag caagtcggca tcggcatcct gctcctcatc
     7141 gcaggcgtat tcgcgatgta ctcgctgtgg atcatctgcg ccgccgcgtc gttctgggtc
     7201 gtgcggctcg acaacctcat ctacttgctg atggcgatct tcgacaccgc gcgctggccg
     7261 atccaggtct tccgcggcgt gtggcgcttc ttgttcacgt tcatcatccc ggtcgcgatc
     7321 atgacgacgt ttccggcaat ggcgctgctc ggcaggctct cgtaccgcac gacgctgttc
     7381 acgctcgccg gtgcgctcgg catggtcttc gtcagccgca tgatctggcg cgctgcgatc
     7441 cgcaactaca cgagcgcgtc gagctagcga gctagagcga ctcgaggaac gcgatcaggt
     7501 cggcgacctg cgagtcactg agcttagcgg tctccgccat gccgtgcacg ttgccgcggt
     7561 cgcggagcaa cgcttcgagc gtcgccgcgc tgccatcgtg gtagtacggc gcgctcgccg
     7621 cgacaccgat gaggctcggc gtatctgcct cgggcagcgt cgccgcctgg aacttgtgct
     7681 tgtcctggtc ggtgaactgc ttgccgtcgt ggcaggtgcg gcaaccgacc tcggccgagt
     7741 cgaacagctt ctcgccgcgc gcgatctgct gcgcatcgtg ggtcggcacg gtcggcttct
     7801 gcagcgtctc gagatacgac gcgagcgcgc cggtctgctc tttcgtgatg ccgaagccgc
     7861 cgaggcgctt catcgtgccg gtgagactcg ccggcaggtc ggcgtcgccg ccatcccact
     7921 tgtacgggtg ggtgtcgacg atgcggcccg cgaggagcgg cgtctgcagc tcgtgcttgt
     7981 cgatgcgcca cgacagacca tccgcgcggc cgtcggggtg gcagctcgcg caggcgagcg
     8041 agccgcgtgc cgagatctgc ggctgcgacg agtggaacag cacgaggccc tcgtgctgct
     8101 tctcgttgag cgacgacgcg acgagcgatt cgcccgcgtc gatcttcgcc gccgtcgcga
     8161 gcttgccctt gccgtcgatc acctcgagcg tcgtcacgct gcgcgtgaac gagcaccaga
     8221 tgaagaggtt gccgtcctga ccgaccgcga tgccgtccgg gccgcagcgg tccttgccct
     8281 tcgtcagcga tgccgacacg ccctcggcga tgccgacctg cgaggcgttc ttgagctgga
     8341 tgatcgagtc gctgccgagg cccgcgacat acagcgcgtc gtgcgccgag tcccacgcga
     8401 gcgcgcgggg ctgatgctgc gagatcgtcg cgacgatctg attcgtgcgc tccttgttcg
     8461 tgccgaggaa cgcgagctga tgcgtgatcg gcgactcgaa
//
LOCUS       DASUFX010000363        13778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_612843,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000363 DASUFX010000000
VERSION     DASUFX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612843"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VFV99_14445"
     CDS             <1..1492
                     /locus_tag="VFV99_14445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060563.1"
                     /translation="QVPEAAIKEKRLLSYTDFKDPALTTMFDRLGSATPEQQQFMHLD
                     ASGDKYLASVSAIGGKRGISDWYMATLIPKSTLFATMKRLNRDSLIASGGALAIAMGV
                     ALMFAWNILRMRRAVGVAREAARSAEARAKQLGSYRLVERLGAGGMGEVWRAEHQLLA
                     RKAAIKLVRPDVLRDRNHAPLIQERFRREAQTLASMRSRHTIELYDYGVTDDGTFFYV
                     MELLDGLDLSDLVREDGPQPAARVISIISQACQSLAEAHDAGLLHRDIKPANLELCKA
                     ADEVDIVKVLDFGIVHNIAEPIAPVSLPLPRPSSNKPQSESGPRLTTEGVVIGTPGYI
                     PPEQAVGAPLDPRGDLYALGCVAWFLLTGSEIYARTSEDEVLRAHVYDAIPNLREKVQ
                     GWLPVELERLITQCLAKQPQQRPEDARTLARMLNAIEIPMEHAWTDVEAQLWWSKHSR
                     TKQVEAVEAERLLVPNRAATTTNIDEHRAGSDARTIDARKSARTVG"
     gene            1489..3174
                     /locus_tag="VFV99_14450"
     CDS             1489..3174
                     /locus_tag="VFV99_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5060564.1"
                     /translation="MKLALALLLVASSAAYAQPVPGSGDDNVREVLIYPYFTNVELPV
                     LAMHGGNPEDTVLLFDGFELPSLFHPGGLRSISTPGAIGEIELLPGAFGVEYGRGSSI
                     VSLSSADGMPRNFGDVTAFDATAHLWMPGRKHSVLGLRSTMLSFRYGWDGFTRPSKLV
                     GGDYLDAVFRQEFDLSNRWSLAESGLFANDDARLLARGVLTAAYKTATWRASVAASAM
                     SLASADLGQLAFDSRAEVIRMADSSAGLTKLEWRLGQQTNSSQFEFGASENPTKRWRH
                     DIAGWSSIAANLSKDIRATVGLRVDNFNGDVATQPRGSLIVQPIKELTVALAAGAYRR
                     PPTQASELAFDLNPERATHVAVGTTYNEPGLRINAIAYYIDRTRLVVHDQLGELHNTG
                     FGTSMGVEAIAQLRGDSWFGTLSSALTRSKRFDYERAKERPAEYEQPFRLDAIAGRSW
                     RRWIFSARLQLASGLPYTPYTGAVYNSDTDTYEPLYVPPLSARTPFHHQVDVRVDYRL
                     VKRSKLVIDGFIDLHNAYRNRGAIGYQYSYDYSERTPITALPLFPFVGLRAYL"
     gene            3171..3821
                     /locus_tag="VFV99_14455"
     CDS             3171..3821
                     /locus_tag="VFV99_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060565.1"
                     /translation="MKRALLLAVAACSEPATLEPWQLDHDRVVAVRAEPPHLASGEIG
                     MFDALIAHEGGPTTVEVARNAAATSAASDLFTAVHFNIDHWQIDGPDEARLAAARAEL
                     GLPDGAPVPLLVSLEFPGPLYADKTMWLGDSAANPAPPAISYGPDLEVGREYQPVFIP
                     PPGSTVRWLTSCGTLTGDTEPRATHIIEEPCTGELVLVLRDPLGGVAWQVMSIVAK"
     gene            3860..4132
                     /locus_tag="VFV99_14460"
     CDS             3860..4132
                     /locus_tag="VFV99_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060566.1"
                     /translation="MRLVVVSFVGLLLAACGNSPEQDSRMVSTAFCDCLHNTPALSEA
                     CIDHDFMPIVPAPSDECLQCVYENSQACLILFDKCFDLCVQLQPQP"
     gene            4158..4919
                     /locus_tag="VFV99_14465"
     CDS             4158..4919
                     /locus_tag="VFV99_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060567.1"
                     /translation="MRCPSLVLVLTAGLGSIAAAQPGPPPAPPPPPADTTTTTTTTDP
                     APPDPPPPPPPPPQQQRAMPVVQEEPVRNDRPDGFSIGLGLGYGLPTSLETPNVTSAR
                     LRLAGGLTFEPLVSIANTTQTTAPPSPAPEAEDKTTEFRIATLVRLPVITHGHVDLEG
                     LAFVGFRNLKDNPDGDFNTTTTNTFAVGWGVGIGYWLSHHWQLSMSVTNPLVAYQSTK
                     ATMDIGGMAVTNKTSETTIGVIFLPQVVAMIHLYN"
     gene            5090..5671
                     /locus_tag="VFV99_14470"
     CDS             5090..5671
                     /locus_tag="VFV99_14470"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5060568.1"
                     /translation="MLILDDNELWLRSAMRELRQSTREDLAAFYARNADEALIHAHRQ
                     VIDLAIVDLHLTSESRDLHCASGISVIKALKATCPPCVAVLTSAHLTTKHVDLARDAG
                     ASFSEPKPVSIMGIIARVEGTLMVTPPSFRTITLARAEWEHIHRVVGDSGNNISEAAR
                     RLGIHRSVLQRKLRRVAPPAHAQTVEETFSSTD"
     gene            complement(5684..6379)
                     /locus_tag="VFV99_14475"
     CDS             complement(5684..6379)
                     /locus_tag="VFV99_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060569.1"
                     /translation="MLRGAAIVCLLAGCDVVWRLDDVHGEVAVDARSDAPGSCAFVDD
                     FTGTMRKQQWDVIIDDPRIMIAQANMTIIDAAANIDTNAETYLRLIERQTLAVGDHVD
                     VEIPQATNTDNGQVETSLLVGQNPQNGYVIDVSQTFLEFYSRVASVGTLASTRMYEPA
                     AHRWWRLTHGPGELDVTFYTSVDGHDWFGQKTVTMATPLVDVEVVLLVGSYNGGASLP
                     TRAIFDNFSICGP"
     gene            complement(6394..7101)
                     /locus_tag="VFV99_14480"
     CDS             complement(6394..7101)
                     /locus_tag="VFV99_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060570.1"
                     /translation="MKRALVIASVVAAGCSSTVDEPWQLDHDRIVAVRATPPSIAAGE
                     QSTIDLLVAYAAAPADVRAPDGAQVISPASLADTLAPGTWTITAPSEDRLVAARAELG
                     LPAGAPVPLEVGVAVAWPYPVTSVDGQMFPATKTVWLGEHRDNPELTAMLVNNAEPGT
                     ELVVPKDDKVPLFVEADDTVDIVNWLTSCGEMHDFDLHQAYLKVLPDQPQDGQLAVVK
                     RDDRGGVSWRVWPIRAE"
     gene            complement(7098..9224)
                     /locus_tag="VFV99_14485"
     CDS             complement(7098..9224)
                     /locus_tag="VFV99_14485"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5060571.1"
                     /translation="MHGRVINSLGRPIRGAKISVEGDTKVVTTDRDGKFHIVAPDGAT
                     LVIDANTYEVGIATIAGPTLDDIVLLTLGQRSETIEIKGEAPAAAPGAAKLDRQELQR
                     LPGTGGDVVRALTVMPGIANQQLPLGSMSGVVIRGSAPQDSKVLVDDFEIPVLMHNIG
                     FRSVLPAEAIQSLEFLPGGFDVAFGRASSGIVSLVTRPGSDQRSTQAEVSMIDGGLLA
                     QGRIGKKTNYMFGLRRSTIDLVLPGLIPDSVDLSLTTVPSYWDGQFRIDHQLTSKWKL
                     ALSGVGTTDTFELYTTKDTESATKRFFNQTRFFRLTGSATYHDGPWTAKLALSGIAPE
                     FTFEFGAFQHIKWRQPAITPRAEVTRTWDKKLGLTNVEWRTGVEAQIGRASFDMASPP
                     DIRDGEQYSGYDPRDTSMSIKDTFWLPDFAGWSSVSANLDPRIRATVGVRTDAFARTG
                     EVAVQPRGELKVKLTPTLTARLSSGAYVRPAEYQTENFSPDARSEHSTQNIVGLQYEP
                     REGIRLQTSAYYTDRTKLLTRETGAGMLENFGRGTTVGAELLATYRGGPWFGWLSYSY
                     SHSTRVDRPGAEERLFNFDQPHSMNAALSWKSGHWQLGGRFQLYSGMPYTPVLGSVFM
                     SDRNLFVPQFGDTNSERAPIHHQLDLRIDYSWKWGPAAMSAFLDVQNVYMNKSIVTYY
                     YGYDFTQRSEFTALPLIPSIGLRGTL"
     gene            complement(9401..10114)
                     /locus_tag="VFV99_14490"
     CDS             complement(9401..10114)
                     /locus_tag="VFV99_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060572.1"
                     /translation="MRWLLLVALLVGCNSDLDEPWQLEHDRIIAIRATPPRILSGETS
                     TIDVLLGFSAAEMPVVERGPDVATVVSPMSLMDTLANDNGNWVVTAPSEERLAAAREE
                     LELEPGAPVPLVVGIAVAWPYEIVTPQGNAFGGAKTLWLGESATNPELTGMLINGIEP
                     GAELVVPKEVKVPLFVEADDKIDIVNWLTSCGEMHDFDLHKAYLKVLPDQPQDGQLAV
                     VKRDDRGGVSWRVWPIRAE"
     gene            complement(10111..12450)
                     /locus_tag="VFV99_14495"
     CDS             complement(10111..12450)
                     /locus_tag="VFV99_14495"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5060573.1"
                     /translation="MRVIVGLAIVCLARAAFAQPEPPTEQPAPEPPKPAPDEPKPADQ
                     PKPADQPTPAPEQAPTPEQPAPLDKTITVRGRVINSLGKPIRGAKISIEGNDEQVATG
                     KDGWFRISAPIGATLVIEASGYEVGIATAAGATLDDIVLLTTAQTSETIEVSGEAPPP
                     SPGAAKLDRQELQRVPGTGGDVVRALTVMPGVVNLQIPLGYSGVVIRGSSPQDSKVLV
                     DDFEIPVLFHNIGFRAVVPAEAIQSLDFIPGGFDVSFGRASSGIVSLVTRPGSDDRTT
                     QAEVSMIDGGLLAQGRIGKKTRYMFGLRRSTIDFVLPALIPESVDLSLTTVPSYYDGQ
                     FRIDHQLNSKWNLTLSSVGTIDTFELYATKDTDSDTKRFFNRTRFVRLTAAARYHDGP
                     WNATLALSGLAPEFIFEAGAFQRINVRQPTITPRAEVTRTWDKALGLTNVEWRTGGEA
                     QVGRTTIDMALPLEMREGEEFMGYDPRDTSQTAKEKFWIPDFAAWSAVSANLDPRIRV
                     TLGVRGDAFTRVGEVTPQPRGEIKVKLAPEWTARLSSGAYRRPPEFQTENMYTNIESE
                     HSTQTIAGLQWEPRDGARVQASAYYTDRTNLITREGMGADLGNNGRGTTVGGELLATY
                     RGGPWFTWLSYSYSHSTRVDHPGDPRRLFTFDQPHSMNAAASWKHRKWQLGGRFQLYS
                     GLPYTPAIGSIFDSDRNIYIPLYGEVNSERAPLHHQLDLRVDYSWKWGPTALTFFVDV
                     QNVYMNQSVVTYFYGYDFTQRTAFTSLPIIPSLGLRGVL"
     gene            complement(12639..13331)
                     /locus_tag="VFV99_14500"
     CDS             complement(12639..13331)
                     /locus_tag="VFV99_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060574.1"
                     /translation="MRPWWLVVLTGCDGVFGLTHIPDRTDAQTGDSEVVIDGREHCEQ
                     DMFPDLTTVLTRWDRATTGVGFDVNVEAGELVVRVPANMPGLAYVRHLANIDMTEATL
                     TVEVPRAIGLIDSETFFSVMIDDTNFYFFDVVNGQLTMRVRSLNVDKHLAMMSYDSAA
                     HRFWRFEHDKVLGQMRFFTSSDGTSFTMRHSMPTDVAVGPMTIELGAATINTGLAVGS
                     EAHFDNLEYCPP"
     gene            complement(13422..>13778)
                     /locus_tag="VFV99_14505"
     CDS             complement(13422..>13778)
                     /locus_tag="VFV99_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX5060575.1"
                     /translation="ALDKLTFEDDRRYGVNIVRRALEEPLRQISGNAGVDGSIVVSKV
                     KEAQGGFGFNAATLEYTDLVKAGVIDPTKVVRTALQNAASVAGLMLTTETLIAEKPKK
                     EEKAGGHEGHGHDFDH"
BASE COUNT         2385 a         4530 c         4440 g         2423 t
ORIGIN      
        1 gcaggtcccc gaggcagcga tcaaggagaa gcgactcctc agctacaccg acttcaaaga
       61 tccagcgctc acgacgatgt tcgacaggct cggcagtgcg acgccggaac agcagcagtt
      121 catgcacctc gacgcgagtg gcgacaagta cctcgcgtcg gtctcggcga tcggcggcaa
      181 acgcggaatc agcgactggt acatggcaac cctgatcccg aagtcgacgc tgttcgcgac
      241 gatgaagcgg ctcaaccgcg actcgctgat cgcttcgggt ggcgcgctcg cgatcgcgat
      301 gggtgtcgcg ctgatgttcg cgtggaacat cctgcgcatg cgtcgcgccg tcggtgtcgc
      361 gcgcgaagca gcccggtccg cggaagcgcg cgcgaagcag ctcggcagct accggctcgt
      421 cgagcggctc ggcgctggcg gcatgggcga ggtctggcgc gccgagcacc agctgctcgc
      481 gcgaaaggcc gcgatcaagc tcgtgcgacc cgacgtgctg cgcgatcgca atcacgcgcc
      541 attgatccag gagcgcttcc gccgcgaagc acaaacgctc gcgtcgatgc gatcgcgcca
      601 cacgatcgag ctctacgact acggcgtcac cgacgacggt acgttcttct acgtgatgga
      661 gctgctcgac gggctcgacc tctccgacct cgtgcgcgag gacgggccgc agccggccgc
      721 gcgcgtgatc agcatcatca gtcaggcgtg tcagtcgctc gccgaagcgc acgacgccgg
      781 tctgctccac cgcgacatca agccggcgaa cctcgagctc tgcaaggccg ccgacgaggt
      841 cgacatcgtc aaggtgctcg acttcggcat cgtccacaac atcgccgagc cgatcgcgcc
      901 ggtgagtctc ccgctgccgc gcccgagcag caacaagccg cagagcgagt cgggtccgcg
      961 cctcacgacc gagggcgtcg tgatcggcac gcccggctac atcccgcccg agcaggcggt
     1021 cggcgcgccg ctcgatccgc gcggcgatct gtatgcgctc ggctgcgtcg cgtggttcct
     1081 gctgaccggc agcgagatct acgcgcgcac gagcgaggac gaggtgctgc gcgcgcacgt
     1141 ctacgatgcg attccgaacc tgcgcgagaa ggtgcaaggg tggctgcccg tcgagctcga
     1201 gcggctcatc acgcagtgcc tcgcgaagca accgcagcag cgccccgagg acgcgcgaac
     1261 gctcgcgcgc atgctgaacg cgatcgagat cccgatggag cacgcgtgga cggacgtcga
     1321 agcgcagctc tggtggagca agcactcgcg cacgaagcag gtagaggcgg tcgaggccga
     1381 gcgcctgctc gttccaaacc gggcggcgac gacgacgaac atcgacgagc acagggctgg
     1441 ctccgacgcg cgcacgatcg atgcgcgcaa gagcgcccgc acagtgggat gaagctcgca
     1501 ctcgcattgc tgctggtcgc gtcgtccgcc gcgtacgcgc agcccgtccc cggtagcggc
     1561 gacgacaacg tgcgcgaggt cctgatctat ccgtatttca cgaacgtgga gctgcccgtg
     1621 ctcgcgatgc acggcggcaa tcccgaggac acggtcctgt tgttcgacgg cttcgagctg
     1681 ccgtcgctgt ttcatcccgg cgggctgcgc tcgatctcga cgccgggtgc gatcggcgag
     1741 atcgagctgc taccgggcgc attcggcgtc gagtacggcc gcggctcgag catcgtgtcg
     1801 ttgtcgtcgg ccgatggaat gccgcgcaac ttcggcgatg tcaccgcgtt cgatgcgacc
     1861 gcacacctgt ggatgccggg gcggaagcac agcgttctcg ggctccgctc gacgatgttg
     1921 tcgtttcgct acggctggga cgggttcaca cgtccgtcga agctcgtcgg tggcgactac
     1981 ctcgatgcgg tgttccgcca ggagttcgac ttgtcgaatc ggtggtcgct cgccgagtca
     2041 ggtctgtttg cgaacgacga tgcgcgtttg ttagcgcgtg gcgtgcttac cgccgcatac
     2101 aagacggcga cttggcgtgc gtcagtggcg gcgtcggcga tgtctcttgc atcggctgac
     2161 ctcggtcagc tcgccttcga ctcgcgcgcc gaggtcattc gcatggccga cagcagcgca
     2221 ggcctcacga agctcgaatg gcgattgggt caacagacga acagcagcca gttcgaattc
     2281 ggagcctccg agaacccgac caagcgttgg cgccatgaca tcgcgggctg gtcgtcaatc
     2341 gctgcaaacc tgtcgaagga tattcgcgca acggtcggcc tgcgcgtcga caacttcaac
     2401 ggcgacgtcg cgacgcagcc gcgcggcagc ctgatcgtcc agccgatcaa ggagctgacg
     2461 gtcgcactcg ccgctggcgc gtatcgccgc ccgccaacgc aagcgagcga gctcgccttc
     2521 gacttgaacc ccgagcgtgc gacccacgtc gcggtcggca cgacctacaa cgagcccggc
     2581 cttcgcatca acgcgatcgc gtactacatc gatcgcacgc gcctcgtggt ccacgaccag
     2641 cttggcgagc ttcacaacac aggcttcggc acgtcgatgg gcgtcgaagc gatcgcacag
     2701 ctgcgcggtg actcgtggtt cggcacgctg tcatccgcgc tcacgcggtc gaagcgcttc
     2761 gactacgagc gcgcgaagga gcgaccagcc gagtacgagc agccgtttcg cctcgacgcg
     2821 atcgccggcc gctcctggcg ccgctggatt ttcagcgcgc gtctgcagct cgcgtcgggc
     2881 ttgccgtaca cgccgtacac cggcgcggtc tacaacagcg acactgacac ctacgagccg
     2941 ctctacgtgc cgccactgag cgcgcgcacg ccgttccatc atcaggtcga cgtgcgcgtc
     3001 gactaccggc tggtcaaacg cagcaagctc gtcatcgacg gcttcatcga cctccacaac
     3061 gcgtatcgca accgcggtgc gatcggctac cagtacagct acgactacag cgagcgcacg
     3121 ccgatcaccg cgctaccgct gtttccgttc gtgggattgc gagcgtatct atgaagcgcg
     3181 cattgctcct cgccgtcgcc gcctgcagcg agcctgcgac gctcgagccg tggcagctcg
     3241 atcacgatcg cgtggtcgcc gtgcgcgccg agccgccgca cctcgcatcc ggcgagatcg
     3301 ggatgttcga cgccctgatc gcgcacgaag gcggcccgac gaccgtcgag gtcgcgcgca
     3361 acgccgccgc gacgtccgcc gccagcgacc tgttcaccgc cgtccacttc aatatcgatc
     3421 actggcaaat cgacggcccc gacgaggctc ggctcgccgc cgcccgcgcc gagctcggct
     3481 tgcccgatgg cgcgccggtg ccgctcctcg tctccctcga gtttcccggc ccgctctacg
     3541 ccgacaagac gatgtggctc ggtgacagcg ccgcgaaccc ggcgccgccg gcgatcagct
     3601 atggccccga cctcgaggtc gggcgcgagt accagcccgt gttcatcccg ccgccgggca
     3661 gcacggtgcg ctggctcaca tcatgtggca cgttgaccgg cgacaccgag ccgcgcgcga
     3721 ctcacatcat cgaggagccg tgcaccggcg agctcgtgtt ggtcctgcga gatccccttg
     3781 gtggtgtcgc gtggcaggtg atgtcgatcg tggccaaata aatttgcgtg cggctgccac
     3841 cgcgcgggac aattttttca tgcgactcgt ggtggtgtcg ttcgttggcc tgttgctggc
     3901 cgcatgcggg aactctccgg aacaagacag ccggatggta tccactgctt tttgcgactg
     3961 cctccacaac acaccggcct tgagcgaggc gtgcatcgac cacgatttca tgccgatcgt
     4021 gccggcgcca tctgacgaat gtttgcagtg cgtctacgag aactcgcagg cgtgcctgat
     4081 cctcttcgac aagtgctttg acctctgcgt gcaactgcaa ccgcaaccgt aatttatcga
     4141 tccactggag gaatgcgatg aggtgtccgt ctttggtgct tgtactgacc gctggtctcg
     4201 gcagcatcgc tgccgcgcag ccggggccac cgcccgcgcc tccgcctccg cccgcggata
     4261 cgacgacaac aacgacgacg accgatccgg cgccgcccga tccgccgccg cctcctccgc
     4321 caccgccgca gcagcagcgc gcgatgccgg tcgttcagga agaaccggtg cgcaacgatc
     4381 gacccgatgg tttctcgatc ggtctcggcc tcggctatgg gctaccgaca tcgctcgaga
     4441 cgccaaacgt caccagcgcg cgccttcgcc ttgcgggtgg cctgacgttc gagccgctag
     4501 tgtcgatcgc gaacacaacc cagaccaccg cgccgccctc gccagccccc gaggcggagg
     4561 acaagaccac cgagttccgc atcgccacgc tcgtgcgttt gccggtcatc acgcatggtc
     4621 atgtcgatct agagggcctc gcgtttgtcg ggttccgcaa cctgaaggac aaccccgacg
     4681 gcgatttcaa cacgactacg acgaacacgt tcgcggtcgg ctggggtgtc ggcatcggct
     4741 actggctgtc gcatcactgg cagctcagca tgagcgtgac gaacccgctc gtcgcctatc
     4801 agagcaccaa ggccacgatg gacatcggag ggatggcggt gaccaacaag acctcggaaa
     4861 cgacgatcgg cgtgatcttc ctgccgcagg tcgtcgcgat gatccacctc tataactgag
     4921 ctgccagaag gtgcagccaa acgctgcacc cgtctgtatc aactcccgag ccggccgcgt
     4981 tgacctcgaa ggaacgagct ggatagagtg aacgtgtcgg gattggatgt ggtcgatgtg
     5041 gtcacgacgt gggggcgtca tgatgcgatc gcttagtcgc gtaaggtcag tgttgatctt
     5101 ggacgataac gagctttggc tgcgctctgc aatgcgtgag ttgcggcaaa gcactcgcga
     5161 ggacttggca gcgttctatg ctcgaaacgc ggacgaagcg ctcatacatg cgcataggca
     5221 agtgatcgac ttagcaattg tggatcttca tctcactagt gaaagccggg accttcactg
     5281 cgcttccgga atttccgtga tcaaggcgct aaaggcgacc tgtccgccat gcgtagcggt
     5341 gctgacgagc gcccacctca ctacaaagca tgttgatctt gcccgtgacg cgggcgcgtc
     5401 gttctcggag cccaagccgg tctccatcat ggggatcatc gcccgggtgg agggaaccct
     5461 gatggtaacg ccgccgtctt ttcgcacgat tacactcgca cgtgccgagt gggagcatat
     5521 ccaccgggtt gtgggagata gcgggaacaa catttccgaa gctgccaggc gacttgggat
     5581 ccaccgctct gtcttgcaac gtaaacttcg acgtgtcgcc ccacctgctc acgctcaaac
     5641 cgtcgaagaa acgttctcca gcactgatta gcgggacggt tcatcacggg ccgcagatgc
     5701 tgaagttgtc gaagatcgcg cgcgtcggta gcgacgcacc gccgttgtag ctgccgacga
     5761 ggagcacgac ctcgacgtcg acgagcggcg tcgccatcgt caccgtcttc tgcccgaacc
     5821 aatcgtggcc gtcgacgctc gtgtagaacg tcacgtcgag ctcgccgggt ccgtgcgtca
     5881 gccgccacca gcgatgcgcg gccggctcgt acatgcgcgt cgaggccagc gtgccgacgc
     5941 tcgcgacgcg cgagtaaaac tcgagaaacg tctgcgatac gtcgatgacg tagccgttct
     6001 gcgggttctg ccccacgagc aacgacgtct cgacctgccc gttgtccgtg ttcgtcgcct
     6061 gcgggatctc gacgtcgaca tggtcgccga ctgcgagcgt ctggcgctcg atgagccgca
     6121 ggtaggtctc ggcattggtg tcgatgttcg ccgcagcgtc gatgatcgtc atgttggcct
     6181 gcgcgatcat gatgcgcgga tcgtcgatga tcacatccca ctgctgctta cgcatcgtgc
     6241 cggtgaagtc gtcgacgaac gcgcaagagc ccggcgcgtc ggacctcgcg tcgacggcaa
     6301 cttcgccatg cacgtcatcg agccgccaca cgacatcgca accggcgagc aagcacacga
     6361 tggcggcccc gcgcagcacg cctcattatc acctcattcg gcgcggatcg gccacacgcg
     6421 ccacgacaca ccgccgcggt cgtcacgctt cacgactgcg agctgaccgt cctgcggctg
     6481 atcgggcaac accttcaagt aggcctgatg gaggtcgaag tcgtgcatct caccgcatga
     6541 cgtgagccag ttcacgatat cgaccgtgtc gtctgcctcg acgaacagcg gcaccttgtc
     6601 gtccttcggc acgacgagct cggtgcccgg ctcggcgttg ttgaccagca tcgcggtgag
     6661 ctccgggttg tcacggtgct caccgagcca cacggtcttc gttgcgggaa acatctggcc
     6721 gtcgaccgac gtcaccggat acggccatgc gacggcgacg cccacctcca gcggcaccgg
     6781 tgcgcccgcg ggcaatccga gctctgcgcg cgcggcaacg aggcgatctt cgctcggcgc
     6841 ggtgatcgtc cacgtcccgg gcgcgagcgt gtcggcgagg ctcgccggcg agatcacttg
     6901 cgcaccgtcg ggggcgcgca cgtcggcggg cgccgccgcg tacgcgacga gcaagtcgat
     6961 cgtcgattgc tcgccggctg cgatgctcgg tggcgttgcg cgcaccgcga cgatgcgatc
     7021 gtgatcgagc tgccacggct cgtcgacagt gctgctgcaa cccgcggcga cgacggacgc
     7081 gatgacgagc gctcgcttca cagcgtacct cgcagaccga tcgacgggat cagcggcagc
     7141 gcggtgaatt cggagcgctg ggtgaagtcg tagccgtagt agtacgtgac gatgctcttg
     7201 ttcatgtaga cgttctggac gtcgaggaat gcgctcattg cggctggccc ccacttccac
     7261 gagtagtcga tgcgcaggtc gagctggtga tggatcggcg cgcgctcgga gttcgtgtcg
     7321 ccgaactgcg gcacgaacag attacgatcg ctcatgaaga ccgagccgag caccggcgtg
     7381 tacggcattc cggagtagag ctggaaccga ccgccgagct gccagtgtcc cgacttccag
     7441 ctcagcgccg cgttcatgct gtgcggttga tcgaagttga acagtcgctc ctcggcgccg
     7501 ggacgatcga cgcgcgtcga atgcgagtac gagtacgaga gccaaccgaa ccatgggcca
     7561 ccgcgatacg tcgcgagcag ctcggcaccg acagtcgtgc cacgaccgaa gttctcgagc
     7621 atgccggcac cggtctcgcg cgtcagcaac ttcgtgcgat cggtgtagta ggccgacgtt
     7681 tgcagccgga tgccctcgcg cggctcgtat tgcaggccga cgatgttttg cgtcgagtgt
     7741 tcggaccgtg catcgggcga aaaattctcg gtctggtact cggcggggcg cacgtacgcg
     7801 cccgatgaca gccgcgcggt caacgtgggc gtgagcttga ccttgagctc gccgcgcggc
     7861 tgcacggcga cttcgccggt gcgcgcgaac gcgtcggtgc gcacacccac cgtcgcgcgg
     7921 atgcgcggat cgagattcgc ggacaccgac gaccagcccg cgaagtcagg cagccagaac
     7981 gtgtccttga tgctcatcga cgtgtcgcgc gggtcgtagc ccgagtattg ctcgccgtcg
     8041 cgaatgtccg gtggtgacgc catgtcgaac gatgcgcggc cgatctgtgc ctcgacaccg
     8101 gtgcgccatt cgacgttcgt caggccgagc ttcttgtccc acgtgcgcgt gacctcggcg
     8161 cgcggcgtga tcgcaggctg gcgccacttg atgtgctgga acgcgccgaa ctcgaacgtg
     8221 aactcgggcg cgatgccgga aagcgcgagc ttcgcggtcc acggcccgtc gtgatacgtc
     8281 gccgagccgg tcaaccggaa aaagcgcgtc tggttgaaga accgcttcgt tgccgactcc
     8341 gtgtccttcg tcgtgtagag ctcgaacgtg tcggtcgtgc cgacgcccga gagcgcgagc
     8401 ttccatttcg aggtgagctg gtggtcgatg cgaaactggc cgtcccaata gctcggcacc
     8461 gtcgtcagcg acagatcgac gctgtcgggg atcaaacccg gcagcacgag gtcgatcgtc
     8521 gagcggcgca ggccgaacat gtaatttgtc ttcttgccga tgcgaccttg tgcgagcagg
     8581 ccgccgtcga tcatcgatac ttcggcttgc gtcgatcgct gatcgctgcc tgggcgcgtg
     8641 acgagcgaga cgatgccact cgacgcgcga ccaaacgcga cgtcgaagcc gccgggaagg
     8701 aattcgagcg actgaatcgc ctcggcgggt agcaccgagc gaaacccgat gttgtgcatc
     8761 agcaccggaa tttcgaaatc atcgacgagc accttcgagt cctgcggcgc gctgccgcgg
     8821 atcacgacgc ccgacatgct gccgagcggc agctgctgat tcgcaatgcc cggcatcacg
     8881 gtcagtgcgc gtacgacgtc gccaccggtg cccggtagtc gctgcagctc ctggcgatcg
     8941 agcttcgcgg cgccgggcgc tgccgcaggt gcctcgcctt tgatttcgat cgtctccgat
     9001 cgctgcccga gtgtgagcag cacgatgtcg tcgagcgtcg ggccggcgat cgtcgcgata
     9061 ccgacctcgt acgtattcgc atcgatcacg agcgttgcgc cgtctggcgc cacgatgtgg
     9121 aacttgccat cgcgatctgt cgtcacgacc ttcgtgtcgc cctcgacgct gatcttcgcg
     9181 ccgcgaatcg gccggccgag cgagttgatc actcggccat gcacggtctg cgcgtcggct
     9241 gctggtgcgt gctgcgaaag ttccgcagcc ggattcgatt gcgccgacgc ggtgccacca
     9301 acgcagcaaa tgacaaatgc agaacgacgt agacgcacga gtcccctctg tgacgtgggg
     9361 cacttcgacg cgtgaagatg cgttcaggtt gcctggcggt tcactcggcg cgaatcggcc
     9421 acacgcgcca cgacacgcct ccgcgatcat cgcgcttcac gactgcgagc tgaccgtcct
     9481 gcggctgatc gggcaagact ttcaagtacg ccttgtggag atcgaagtcg tgcatctcac
     9541 cgcacgacgt cagccagttg acgatatcga tcttgtcgtc ggcttcgacg aagagcggaa
     9601 ccttgacttc cttcggcacg acgagctcgg cgccgggctc gatgccgttg atcagcatgc
     9661 cggtgagctc gggattcgtc gcactctcgc cgagccacag cgtcttcgca ccaccgaatg
     9721 cgttgccttg cggggttacg atctcgtacg gccacgccac cgcgatgccg actaccaacg
     9781 gcaccggcgc gccgggctcg agctcgagtt cttcgcgcgc ggccgcgagc cgctcttcac
     9841 tcggcgcggt gacgacccag ttgccattat cgttcgcgag cgtgtccatc aggctcatcg
     9901 gagaaacgac ggtcgcgaca tccgggccac gctcgacgac tggcatttcg gctgccgaga
     9961 agccgagcag cacgtcgatc gtcgacgtct cacccgacaa gatacgcggc ggcgttgcac
    10021 ggatcgcgat gatgcggtcg tgctcgagct gccacggctc gtcgagatcc gagttgcaac
    10081 caacaagcaa cgcgacgagt agcaaccacc tcacagcaca cctcgcaatc cgagcgacgg
    10141 aatgattggc aacgacgtga acgcagtgcg ctgcgtgaag tcgtaaccgt agaagtacgt
    10201 caccacactc tggttcatat acacgttctg aacatcgacg aagaacgtga gcgcggtcgg
    10261 gccccacttc cacgagtagt cgacgcggag gtcgagctgg tggtggagcg gcgcgcgttc
    10321 ggagttcacc tcgccataga gcggaatgta gatgttgcga tcgctgtcga agatcgagcc
    10381 gatcgctggc gtgtacggca ggccggagta gagctggaac cgaccgccga gctgccactt
    10441 cctgtgcttc cagctcgcgg ctgcattcat gctgtgcggc tggtcgaacg tgaacagccg
    10501 gcgaggatcg ccgggatgat cgacgcgcgt cgagtgcgag tacgagtagg acagccacgt
    10561 gaaccacggg ccgccgcggt acgtcgcgag cagctcgccg ccgacggttg tgccccggcc
    10621 attgttgccg aggtcggcgc ccataccctc gcgcgtgatg aggttcgtgc ggtcggtgta
    10681 gtacgccgat gcctgcacgc gggcaccgtc acgcggctcc cactgcaggc ccgcgatggt
    10741 ctgcgtcgag tgttccgact cgatgttcgt gtacatgttc tcggtctgga actccggcgg
    10801 ccggcgatac gcacccgacg acagccgcgc cgtccattcg ggcgccagct tcactttgat
    10861 ctcgccgcgc ggctgcggcg tcacttctcc gacgcgcgtg aacgcatcgc cgcgcacacc
    10921 gagcgtgacg cgaatccgcg gatcgaggtt cgccgacact gcgctccacg ccgcgaagtc
    10981 cggaatccag aacttctcct tcgcggtctg cgacgtgtcc cgcgggtcgt agcccatgaa
    11041 ctcttcgccc tcgcgcattt cgagcggcag ggccatgtcg atcgtcgtgc gcccgacctg
    11101 ggcttcaccg ccggtacgcc actcgacgtt ggtcagcccg agcgccttgt cccacgtgcg
    11161 cgtgacttcc gcgcgcggag tgatcgtcgg ctggcgcacg ttgatgcgct ggaacgcacc
    11221 ggcctcgaag atgaactcgg gcgcgagacc cgacagcgcg agggtcgcgt tccacggacc
    11281 gtcgtgatag cgcgccgccg cggtgaggcg cacgaagcga gtgcggttga agaaccgctt
    11341 cgtgtcggag tcggtgtcct tcgtcgcgta cagctcgaac gtatcgatcg tgccgacgct
    11401 cgagagcgtg aggttccact tggaattcag ctggtgatcg atgcggaact ggccgtcgta
    11461 gtagctcggc accgtcgtca gcgacaggtc gacgctctcg gggatcaacg ccggcagcac
    11521 gaagtcgatc gtcgagcggc ggagcccgaa catgtagcga gtcttcttgc cgatgcggcc
    11581 ctgcgcgagc aggccgccgt cgatcatcga gacctcggct tgcgtcgttc ggtcgtcgct
    11641 acccggccgc gtgaccagcg acacgatgcc gctcgatgcg cgtccgaacg agacgtcgaa
    11701 gccgccggga atgaagtcga gcgactggat cgcttcggcc ggcacgaccg cgcggaagcc
    11761 gatgttgtgg aacagcaccg gaatctcgaa gtcgtcgacg aggaccttcg agtcttgcgg
    11821 tgagctgccg cggatcacga cgcccgagta gccgagcgga atctgcagat tgacgacgcc
    11881 gggcatcacg gtcagcgcac gcacgacgtc gccgccggtg ccgggcacgc gttgcagctc
    11941 ctggcgatcg agcttggcgg cgccgggcga gggcggtggc gcttcaccgc tgacctcgat
    12001 cgtctccgag gtctgcgcgg tcgtgagcag cacgatgtca tcgagcgtcg caccggcggc
    12061 cgtcgcgatg ccgacctcgt agccactcgc ttcgatcacg agcgtcgcgc caatcggcgc
    12121 ggagatccgg aaccagccgt ccttgccggt cgcgacctgc tcgtcgttcc cttcgatgct
    12181 gatcttcgcg ccacggatcg gcttgccgag cgagttgatc acgcggccgc ggaccgtgat
    12241 cgtcttgtcg agcggcgcag gctgctcggg agtcggcgct tgctcgggcg ccggcgtcgg
    12301 ctggtcggcc ggtttgggct ggtcggccgg cttcggctcg tccggcgcgg gcttgggtgg
    12361 ctccggcgcg ggctgttcag ttggaggctc gggctgcgca aacgcagcgc gagcaaggca
    12421 aacgatggca agtcctacga ttacccgcac aagacctcga agcgacagca atgaccatgc
    12481 ccgaaccgac gcattgatcg acaccagctt agaacgaatg accgcatgcc gatccgacag
    12541 gtgcagtgaa ttccgacgcg aaacccaggg cacattgtcg cgtgttcccg tcgtgtgtcc
    12601 ccgctgacgc gaaaaacgtt caatcccgcg aattagcgtt acggcgggca gtattcgagg
    12661 ttgtcgaagt gcgcttcgct gccgactgca agcccggtgt tgatggtggc cgcgccaagc
    12721 tcgatcgtca tcgggccaac ggcgacatcg gtgggcatgg aatgccgcat cgtgaagctc
    12781 gtgccgtcgg agctggtgaa gaaccgcatc tggcccagga ccttgtcgtg ctcgaaccgc
    12841 cagaaccgat gtgcggcgga atcgtagctc atcatcgcga ggtgcttgtc gacgttgagg
    12901 ctgcgcacac gcatcgtgag ctggccgttg acgacgtcga agaagtagaa gttcgtgtcg
    12961 tcgatcatga ccgagaagaa cgtctcggag tcgatgaggc cgatcgcgcg tggcacctcg
    13021 acggtaagcg tcgcctcggt catgtcgatg ttcgcgaggt gacgcacgta cgccagtccc
    13081 ggcatattcg ccggcacccg cacgacgagc tctccggcct ccacgttcac gtcgaagccg
    13141 acgccagtcg tggcgcggtc ccaccgtgtg agcacggtag tgaggtcggg aaacatgtct
    13201 tgttcgcagt gctcgcgccc gtcgatcacg acctccgagt caccggtctg tgcatccgtg
    13261 cgatcgggaa tgtgcgttag cccgaatacg ccgtcgcatc cggtgagcac gacaagccac
    13321 cacggcctca cacctcatca tagcgcgaac cgaaaatgcg aagagccggc ccggaggcca
    13381 gctcttcaat cagtcagcga aatcgatcgg gcttggcgcg atcagtgatc gaagtcgtgg
    13441 ccgtggccct catggccacc cgccttctct tccttcttcg gcttctcggc gatcagcgtc
    13501 tccgtggtga gcatgaggcc cgccaccgag gccgcgttct gcagcgcggt gcggacgacc
    13561 ttcgtcgggt cgatcacgcc ggccttgacg aggtccgtgt actcgagggt cgcggcgttg
    13621 aagccgaagc cgccctgagc ctctttgacc ttcgacacga cgatcgagcc gtcgacgccc
    13681 gcgttgccag agatctggcg cagcggctcc tcgagcgcgc ggcggacgat gttgacgccg
    13741 tagcggcggt catcctcgaa cgtcagcttg tcgagcgc
//
LOCUS       DASUFX010000364        17509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_892119,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000364 DASUFX010000000
VERSION     DASUFX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17509
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892119"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFV99_14510"
     CDS             <1..377
                     /locus_tag="VFV99_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060576.1"
                     /translation="AGGRARDHRSYAAISGIATHGGAVAKIEELLDIGEHGWTFAHEL
                     AHLVFFHLDEKRARPLRALYDLAVDVGYANVEYALSNVDEFFAVGYADYLRNRYDLPG
                     APHADDAGVQHALAKYFDSLCA"
     gene            670..1830
                     /locus_tag="VFV99_14515"
     CDS             670..1830
                     /locus_tag="VFV99_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060577.1"
                     /translation="MRWVALLILCVACSDRYGAYLVVDSHGAVTFDRVEFYFGKELGG
                     PLPTSPKHPLVSTEPSLVAKRLFVDADVQLAGGATTMITYYVPDQPENENLGRYVVVL
                     AYSGDQIVGIGELFDFEVMTGSAVYRYDVDLVDARAEDIELWGRPTADCVRWTRDRGT
                     GKPRTVAVVRGQDADCDSFLEGNIANQDCLPRVFCDGSGNAACDARVPCLTEGGESGC
                     QVGTCSNKDGAPASCIPTTCVDENVCAPCDMTPAQDVPIGCALLNNSVHVDTPIEVNP
                     DYTLCTEPYKIDMQMPVECLNPAIVSVIDYMPADPFTFSIADATGNTCQLTITPKMPG
                     AHFTTVPHMMIAIDTPMGTAPRSAFIMGISANAGTCQTSTIMPDVRFGSCVP"
     gene            complement(1843..2904)
                     /locus_tag="VFV99_14520"
     CDS             complement(1843..2904)
                     /locus_tag="VFV99_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060578.1"
                     /translation="MRDADAYPQYQLSHDPTCTACHLTPAGGGLLTENGLMVAENESW
                     KGTDPTFFYGAPLPSWLRMSGDIRGAAGAVDNGVFNGAAYPMQAEAYASAGARGFSLN
                     LTGGFRRPQDGGSPAHVVWSREHFLMWQQHPEDPYGLYVRVGRLMPVFGLRLAEHVVY
                     TQRFGGYPLYGEAYAAAVAYVSARFEAHLTGFIHDPIGSPVEHGDGGAGYVEVRLGKD
                     KHAQVGAEGKYAAADDLHRTYVGLTGKVYFPSVDLLVQAEAQLVHQEIVSTSYTYNQL
                     IGYVLASRPIVHGWLLDVGVGHFTQNTDVDGNYRDCIDANVHWFVTSHLELLATTRLE
                     LLGSGPNGGYALAQLHYRL"
     gene            complement(2961..3272)
                     /locus_tag="VFV99_14525"
     CDS             complement(2961..3272)
                     /locus_tag="VFV99_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060579.1"
                     /translation="MSVLIGCGTTDERPATVEVVSLEILAPTCGAVQCHSTTTRTEGL
                     AFDTLDEARASMRDLGVRARDGGELLEVINETEMPPDAPMFENDIRLIEKWIAAGAPG
                     L"
     gene            3640..5859
                     /locus_tag="VFV99_14530"
     CDS             3640..5859
                     /locus_tag="VFV99_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5060580.1"
                     /translation="MELETDAYRLLFDANPLPMFVSDHDTLQIIEVNRAGVELYGWTR
                     DELTAMTLRDIRPPTDVEFFEASFADSRKTGAETYSRAARHRTKDGRIIDVRLDISAI
                     TLNGRNVGLVIITNMTGIEEAERRFKLLVENSSDGISLTTADNVVQYVSPGGLRILGY
                     DEATVIGSPAEAVTHPDDARGWDVPGLGEIRHYVARLKHRDGSWRWIESSTKNLTHDP
                     AVRAYVSNYRDITERKLAEVALRKSETNFRTLIECAPAATFVHRDGRFVYVNPAAAAM
                     LGHERPDEIVGRLVSDYVHPDEREEITRRLELLTEIGQLRVGQVRMQRADGSPFVIEG
                     EAMLLDFDGVPSHVVIGHDVTERDAMFARIAIADRMLSIGTLAAGVAHEINNPLAYIA
                     TNLELLAAELPNIRPSSRSRLTDATLQCLVTDARDGVARVNSIVRDLRSLSRTEDQKP
                     GPVCVAGVLASSIKMTSNEVRHRARVVQEYADNLPAVHADASRLGQVFVNLLLNAAQA
                     IVEGDADRNEIRVSVRTSDDGTRVRVDIADTGTGIAPSIIGRIFDPFFTTKAPGAGTG
                     LGLAISHQIVRSMGGDITVASQLGKGSTFSVVMPVAEPASADAEDTCAGTASVNRRIL
                     LVDDEAAVGRSLQLLLAPETEVIAVLSGEQALARLTAGEHFDAIVCDIMMPQSTGMQL
                     YDDVARVAPEYRSRMIFMTGGAFTPQARAFLTKLDRPYLEKPFSEQELRDALARVKA"
     gene            5896..7203
                     /locus_tag="VFV99_14535"
     CDS             5896..7203
                     /locus_tag="VFV99_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HEX5060581.1"
                     /translation="MALRVAGVVLALAATASADRLSPVDLARKNDGGYVTGLPLVSYS
                     TDLGFGGGARAYYYWNGHRDDARFANTPYLFRVFLQAYATTGGLQFHWLDLDAPRFDS
                     SPYRIRSQLIFARNTNSNYFGHGARSLDPLRFPGSAAFDSYADYAAAQHQIMGGETFA
                     KYDQYDLLEPNGIAIIERGFDNDRVRVLAGLGFSYARIHDYTGKTVDATSGQATEAQT
                     RLTTDCAAQLLVGCSGGRDDHLRVGVSYDTRDFEPDPNRGVFLDAVLDVGTAALGSEY
                     DYVRFLVAARGYYSPIPDRTDLVLATRAAFEWQSSGTPFFSMDTIPFTEDPRQGLGGH
                     RTLRGYRQDRFVGPVMALVNQEARWTFARTTLWRQKLAFIAVGFLDVGRPFDRVSELS
                     LRRWRPSYGGAMRISWNLATVITVDYGVSPEDSGLYINFNHIF"
     gene            complement(7214..9436)
                     /locus_tag="VFV99_14540"
     CDS             complement(7214..9436)
                     /locus_tag="VFV99_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060582.1"
                     /translation="MARALALLSLLVVLGCDDSSAPRAVDGVLDLRGWELDDTSVLSL
                     QGGWTFYWKKLIEPGDNTAPDGVLPIGRWNGQKLTDGRELGGDGFATYRLIVHLPETP
                     KQYALATAPVVDANRFVVSRLDGQLLAEPLRSGTVGTTSDQSQGTPRNAVVSFTAGGD
                     VLLTLQVSNFEHARGGSGPAPTLGTAAAIGQRERHRRMTDFFVLGLLILVGVQQVVQF
                     VMNRADRASLWFGLVCLVIAGRVFILGRYPADEVPGFSTVMSLRLEYLTGYLGSPLIA
                     LFIRAVFPTYVHALAVRGFVAASLVFALTVALPAPIFTLLQTAYQMLIFAALAYAIVS
                     LARAVIGKRDLPSVLMLVGFVLLALAVAWDVVRVRRVVQPLQATTQYGLTAFVLCQST
                     LLAFLNRRRSRELEQRNRDVQLLNEELRQQIANRSHELSHALTLMASPKRSAQELDPG
                     TVLAEKYRIDQLLGEGGMGKVYRAERLSDGRAVAIKLVRGGSAGKLARFAREAELVAR
                     IDHPNVVSVLDFGITDQAVLFLVLELIEGNSLEHVRDRFGDLSWAVPMVAQLAKAIET
                     IHALGVIHRDIKPANVLVAGDVLKLTDFGVAHVGRPDDPERAEAQTVVEAEPDPDETA
                     TLNPSLTKVGMLIGSPRYMAPELGQGADHASPRSDLFSFGIVAYEMLSGQRPYDRPVV
                     KAFLAGKPMPDPAQPLASVVPGLPADVAAIVDACLQIDAKARPDAATVAKVFAGACAR
                     "
     gene            complement(9485..11914)
                     /locus_tag="VFV99_14545"
     CDS             complement(9485..11914)
                     /locus_tag="VFV99_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5060583.1"
                     /translation="MRTQRYVQWVQRHTVAIFTSSLVLVGIAIYLAAFHLPLQTDFSS
                     LLPGDTPSVKAAEKLAKRAPSRDTMLVVIVAPDPKSREAAATEALAGISALGPELVES
                     ADGDDVATRAFIREHRELYVPMNELTAVRDALAKQIADAKLHANPLFIDIDDEPAPQD
                     THELDELRKKQKDADAQLARDRHVSADGRSQVIVVHTAFVATDVDRDRKLMGALDKVA
                     MKIRGAHPFVTVGFAGGPAVSLAEHGALSRGIVMSSLLTGLLVSLVLFLHLRSKRALA
                     LLGFNILIATLIAFGAAALTVGHLNAATAFLGAIIAGNGVNYGILLIARYLEERRTAN
                     VTQAMTTAIRGTLMPTLVASLGAAIAYGALGATKFRGFADFALIGGVGMIVCWICSFV
                     LLPAMIMRFAGNSMREPSPMFGRIVVKLFGFRRPVLVTGVAAAITIGACVISWHYLTH
                     DPYEYDLTQLRSQAPDARATRDWLKYSDDEFGRGLAGLAGQTFVAVDRKEQVPSVVAQ
                     LHAIAKQEPMVGSVSSALDVIPQDQAAKLALLAEIRDQIDEIAPQLDEQQRAELLALR
                     PADTLAAVTAQDLPPALARKLTERDGRVGLMIAVKPSAEFDERDGRDLIRFSGAVRKL
                     SHDDVTASGACMLFADVLTQIQKDGPLVTAIAAIALIAMVVLIVGRSRRAVAVLVASS
                     AGSIGMIAVCALAGLKINFLDFVALPITLGLGIDYAINIADRAAHGDPMIALRSTGGT
                     VLVCSLTTVIGYMSLLVSDNLSIRGFGLASLIGELTCVVAAFVIVPAIIALPKLSEAP
                     EFGTDAASSRA"
     gene            complement(12056..13564)
                     /locus_tag="VFV99_14550"
     CDS             complement(12056..13564)
                     /locus_tag="VFV99_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060584.1"
                     /translation="MRHALALVVLVGCTDNTVVVTPIIDIPANDDAQPLDLDTITLSI
                     AHAGAADDITSATFSHGDVIELPNVPYGDDLVVHMVGRRSNSEYAYGRTCEFSVRPDA
                     RLPTPHLFFARQVKFGEMSQQPLPRIGGQAMMYRDGSGLLVGGEYPDENNKPLALTQI
                     ERFDPSTGAYDNLHDIERRYDAAVAIVGTGADSRMVIVGGLDPATGVGAEFAEVIEAE
                     RNTDRQYEKFFEPRLNRTRLTATTLSDGRAVVIGGYQVPDPAKAIAGGPSNIVAQVSV
                     DTGTTAITALRAQLKYPRYSHTATRLTDELGAAVLVAGGLDANGKPIKEAELYKPLFD
                     NFSDQFTAQMIVPRWNHQVARLPDGSVLFIGGLTINPMTGQTEIADQLELFSPLDGRF
                     ISVGLLSEKAPTAGRIGMSATVLPDGRVLITGGAITVDADGKPGPPVDTAFIASLDPL
                     DGQLRVVATDNLAVPRMNHQATPLCDGTVLISGGTVGQATYERYNPAAIGRR"
     gene            complement(13561..14577)
                     /locus_tag="VFV99_14555"
     CDS             complement(13561..14577)
                     /locus_tag="VFV99_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060585.1"
                     /translation="MARADAAPTADLVVVWAPGLRTAPVVQAARGAGAAILDRSPKPE
                     GTATTAQLVQKGLDAFERLEFDQAWQQLDSARSEVDRTGAAGLTQAQLSDLFLYRGLI
                     KTQQGDSNGAWEELIVANTVDATRELDPGRFPPKIIAEFERAQATVKNKGGAELTLTV
                     PQGCRATVDATNVNVATAIPLIVGRHWVRVTCPDREPDGRRVELVGGKITLPITPPEF
                     AAPTDTELLGQAQRSGARAFIVAEVRNNVATARLVGLDGKERDRKTVTIGTDLEPLAD
                     AVSALLTPRAHRPWYKSKWAWAVGGAVGAAAIVIPLTIFATQDSGSPAFTARPDWPDR
                     YVPL"
     gene            14674..16353
                     /locus_tag="VFV99_14560"
     CDS             14674..16353
                     /locus_tag="VFV99_14560"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060586.1"
                     /translation="MSRALGRYELLRPLARGGMGEVYLARRRAAGVEKWLVVKRMRPE
                     RAGDARFLDLFMREARLSMSLAHQNIVPVFDFGRIDDQVFLAMERVEGKDLGSSMTKA
                     GKGMPPLLAAFIAAECCQALDYAHRRKGPDGADLGIVHRDVTPRNVLLSWSGEVKLTD
                     FGIAALAGDEPSKMAGTPAYMAPEQARSEPTDVRADIYAVGMILREALTGARGRHGND
                     RETVLAAAQAGKLDPWPEGFDSPLKAVVDRATATAKEDRYPDARAMLEDLDDFIVAER
                     AAKKAEAPARQLAAWLAEVWQGERDDVTVSDVDIEAKHMMSFLDDGQFDVVGTGTMRS
                     MAMTAAEDVLSKHSEVKADATPAEVTPSPRAPTAGERPDTATPAPQRRSKLPWILLVA
                     VVVAASAIVVAMSMNKPEQPERVVEPPIAKVEADAHEPIVEPEAVDAVVAPPADAGVA
                     DAPARVKPHNTQHVVTPRPDAHVAAPPASATKYKVTISTLPKWSNFTVDNDPTVYQSF
                     DSIQLAPGPHKVHFTGSVHYPADKIVTITVPEKDFKTTVTLDAPEKDAPAP"
     gene            complement(16301..>17509)
                     /locus_tag="VFV99_14565"
     CDS             complement(16301..>17509)
                     /locus_tag="VFV99_14565"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5060587.1"
                     /translation="YPIRSVLARQKNARVLLADVKSIDVDKRTVTLDAGSLHYDFLVV
                     ATGATHSYFGKNEWEARAPGLKSIEDALEIRRRLLVAYEAAERESDLEREREWLTFAV
                     VGGGPTGVELAGALGEIGLHTLAKDFRSIDPTSLRVLLFEGRDRLLNAYPEKLSEAAK
                     QSLEKRQVSVRLNTMVTSIDERSITVKGPAGEERIGTRTVLWAAGVQASPLAKSLGAP
                     LDRTGRVLVNPDLSIPGHPEVFVIGDLAKMMSDGVEVPGVAQGALQSGKHVAKIIDSE
                     ARGEGVRSAFHYRDKGNMATIGRADAVIATKRFAKHGLLAWMLWWVVHIFFLVGFRNR
                     LFMMFHWAWSWLTYRRESRLITGDVGKLPAVTTIADDGTVALPPAAENIAVTTEPEHP
                     SREHRASRSS"
BASE COUNT         3081 a         5847 c         5717 g         2864 t
ORIGIN      
        1 cggccggcgg acgcgcgcgc gatcatcgga gttatgcagc catctcgggt atcgcgaccc
       61 acggcggtgc ggttgcgaag atcgaagaac tgctcgacat cggcgagcac ggctggacct
      121 tcgcgcacga gctcgcgcac ctcgtgttct ttcatctcga cgagaagcga gcgcgaccac
      181 tgcgcgctct ttacgatctt gcggtcgatg ttgggtacgc gaacgtcgag tacgcgctat
      241 cgaacgtgga cgagtttttc gccgtcggct acgcggacta tctgcgtaat cgctacgacc
      301 ttccgggggc accgcacgca gacgatgccg gcgtgcagca cgcgctcgcg aagtacttcg
      361 actcactctg tgcgtgacgg tcgatcgcgt tagcgcggca cgtagccgag gtgaagagcg
      421 cggtgcgcag gacgccgggc gtgatgagca catcgcggat tacatccacc cgccgcgacc
      481 tgcgcgcgcc agtcgcgatc tgctgctcgt accagcggtg tcgaagaggt cctcgacgga
      541 gaggacgacc tcgaacatcg ctttgcaggc cgcctacaag atggagaagt acggcctcaa
      601 gccgcctccg tcgcgcacga ctaaccgagg ggcatcgaac ccgcccgcca ggtcggctac
      661 actggatcgg tgcgttgggt cgcgctgctg atcctgtgcg tagcgtgttc tgatcgctac
      721 ggcgcgtacc tcgtggtcga cagccacggt gcggtgacgt tcgatcgcgt cgagttctac
      781 ttcggcaaag agctcggtgg cccgctgccg acctcgccga agcatccact ggtgagcacc
      841 gagccatcgc tcgtcgcaaa gcgcttgttc gtcgacgccg acgtccaatt ggcgggcggc
      901 gcgaccacca tgatcacgta ctacgttccg gatcagccgg agaacgagaa cctcggccgc
      961 tacgtcgtgg tgctcgcgta cagcggggat cagatcgttg gcatcggcga gctgttcgac
     1021 ttcgaagtga tgaccggcag cgccgtgtac cgctacgatg tcgacctcgt cgatgcgcgc
     1081 gcggaagaca tcgagctatg gggaagacca actgccgact gcgtgcgctg gacgcgcgac
     1141 cgcggaacgg gcaagccacg cacggtcgcc gtggtgcgcg gccaggacgc cgattgcgac
     1201 agctttctcg agggcaacat cgcgaaccag gactgcctgc cgcgcgtgtt ctgcgacggc
     1261 agcggcaacg cggcgtgcga cgcacgggtt ccgtgtctca ccgaaggcgg tgagtccggc
     1321 tgtcaggtcg gcacctgcag caacaaggat ggcgcacctg cttcatgcat ccccaccaca
     1381 tgcgtcgacg aaaacgtctg tgccccctgc gatatgacgc ctgcgcagga cgtgccgatc
     1441 ggatgcgcgc tgctcaacaa cagcgttcat gtcgatacgc cgatcgaggt caaccccgac
     1501 tacacgctgt gcaccgagcc atacaagatc gacatgcaga tgccggtcga gtgtctcaac
     1561 ccggccatcg tcagcgtcat cgactacatg ccggccgacc cgttcacgtt ttcgatcgcg
     1621 gatgcgaccg ggaacacgtg ccagttgacg atcacgccga agatgcccgg cgcgcacttc
     1681 acgacggtcc cgcacatgat gatcgcgatc gatacgccga tgggtacggc gccacgcagc
     1741 gccttcatca tgggcatctc tgcgaacgcg ggcacgtgcc agacaagcac gatcatgccg
     1801 gatgttcgct tcggcagctg cgtgccctga ccgccgtgtg acctacaggc ggtagtgcag
     1861 ctgcgcgagg gcgtagccgc cgttcggtcc actgccgagc agctcgagcc gcgtcgtcgc
     1921 cagcagctcg aggtgtgacg tgacgaacca gtgcacgttt gcatcgatgc agtcgcggta
     1981 gttgccgtcg acgtccgtgt tctgcgtgaa gtgcccaacg ccgacatcga gcagccaccc
     2041 gtgcacgatg gggcgcgagg cgagcacgta accgatcagt tggttgtacg tgtagctcgt
     2101 cgagacgatc tcttgatgca cgagctgcgc ctcggcctgg acgagcagat ctacgctcgg
     2161 gaagtacacc ttgcccgtga gcccgacgta cgtgcgatgc agatcgtcgg cggcggcgta
     2221 cttcccttcc gcgcccacct gagcgtgctt gtctttgccg agccgaacct ccacgtagcc
     2281 cgcgccgccg tcgccgtgct cgaccgggct tccgatcgga tcgtggatga agcccgtgag
     2341 gtgagcttcg aagcgcgccg acacgtacgc gaccgctgca gcgtaggcct cgccgtacag
     2401 cggatagccg ccgaaccgtt gcgtgtagac gacgtgctcg gcgaggcgca gcccgaagac
     2461 gggcatcaac cggcccacgc gcacgtacag cccgtacggg tcctcgggat gttgctgcca
     2521 catgagaaag tgctcgcgcg accacacgac gtgcgccggg ctgccaccat cctgcggacg
     2581 ccggaagcca ccggtcaggt tgagcgagaa gccccgcgcg ccggcagacg cgtatgcctc
     2641 cgcttgcatc ggataggcgg cgccgttgaa gactccgttg tccaccgcgc cggccgcgcc
     2701 acgaatatca ccgctcatcc gcaaccagct cgggagcggg gcgccgtaga agaacgtagg
     2761 atccgtccct ttccacgact cgttctccgc gaccatcaga ccattctcgg tcaggaggcc
     2821 accgccggcg ggggtcaagt gacacgccgt gcaggtcggg tcatgcgaga gctggtactg
     2881 cgggtacgcg tccgcatcac gcattcccgc cacactcacg acaagcattg ctgccacact
     2941 cacaaagcgc aggatggctt tcatagtccc ggcgctcctg cagcgatcca cttctcgatc
     3001 agccggatgt cgttctcgaa catcggcgcg tccggcggca tctcggtctc gttgatgact
     3061 tcgagtagct cgccgccgtc gcgcgcgcgt acgccgaggt cgcgcatcga tgccctggcc
     3121 tcatcgagag tatcgaaggc aagcccttcg gtccgcgtgg tcgtcgagtg gcactgaacg
     3181 gcaccgcagg tcggggcaag aatttcgagc gacaccactt cgacggtcgc gggccgctcg
     3241 tcggtggtcc cacagccgat cagtacgctc aacgcaaggc tacgtagtgt acgaacgctg
     3301 cacgaaaaac gaggaaatgt tcgcatcgcc gcagccaagc tgcatctcga gtgccggaga
     3361 actcggccga aagcgcgcaa ggcctgcgaa gtgatcgagg cgcgacaccc gtcagatccg
     3421 cacgttcgag gtagcgaagc agcagggcaa gcccgactcg acgtacgagg ttggtacacc
     3481 gcgcgaagtg gcatgcggcg aacgcctccg cgcagatcag gcactcgtgc tggtcgccgg
     3541 cgacaaagat gcgcccttgc ggaaggcatc cggccgtgac cgcgtcggtc gtcgggtagc
     3601 gggcggttac ccctccgacg ctcatgctac gagatcgata tggagctcga aacggatgcc
     3661 taccgtctgt tgttcgatgc gaacccgctg ccgatgttcg tctcggacca cgacacgctc
     3721 cagatcatcg aggtgaaccg cgcaggcgtc gagctctatg gctggacgcg cgacgagctc
     3781 accgcgatga ccctccgcga catccgtcca cccacggatg tcgagttctt cgaggcctcg
     3841 ttcgccgatt cgaggaagac gggcgcggaa acgtactcgc gtgcggcgcg ccatcgcacg
     3901 aaagacggcc gcatcatcga tgtcaggctc gacatctcgg cgatcacatt gaacggccgc
     3961 aacgtagggc tcgtgatcat caccaacatg accggcatcg aagaagcgga gcgccggttc
     4021 aagctgctcg tcgaaaactc cagcgacggc atctcgctga ccaccgcgga caacgtcgtg
     4081 cagtacgtga gcccgggtgg cctccgcatc ctcggctatg acgaagcgac ggtcatcggg
     4141 agccctgccg aagccgtgac gcaccccgac gatgcccgcg gctgggatgt gcctgggctc
     4201 ggcgagatcc gacactacgt cgcgcgcctc aagcatcgcg atggttcgtg gcgatggatc
     4261 gaatcatcga cgaagaacct gacgcacgat cccgcggtgc gcgcgtacgt gtcgaactac
     4321 cgcgacatca ccgaacgcaa actggccgag gttgcactcc gcaagtccga gacaaacttc
     4381 cgcaccctga tcgaatgcgc gccagcggca acgttcgtgc accgagacgg gcggttcgtc
     4441 tacgtgaacc cagccgcggc tgcgatgctc ggccacgaac gccccgacga gatcgtcggc
     4501 cggctcgtca gcgattacgt gcatcccgac gaacgcgagg agatcacccg gcgactcgag
     4561 ctgctcaccg agatcggcca gcttcgcgtc ggtcaggtcc gcatgcagcg cgccgacggc
     4621 tcgccgttcg tgatcgaggg cgaagcgatg ctgctcgatt tcgacggtgt gccgtcgcat
     4681 gtcgtcatcg gccacgacgt gaccgagcgc gatgcgatgt tcgcgcgcat cgcgatagct
     4741 gaccgtatgc tgtcgatcgg tacgctcgcg gcaggcgtcg cgcacgagat caacaatccg
     4801 ctggcgtaca tcgcgacgaa tctcgagctg ctcgcggccg agctaccgaa catccggccg
     4861 agcagtcgct cgcgactcac cgacgcgacg ttgcagtgcc tcgtcaccga tgcccgcgac
     4921 ggcgtcgccc gtgtgaactc gatcgtgcgc gacctgcgct cgctatcccg gaccgaagac
     4981 cagaagcccg gccccgtctg cgttgccggc gtgctcgcct cgtcgatcaa gatgacgagt
     5041 aacgaggtcc gtcaccgcgc acgtgtggtg caggaatacg cggacaactt gcccgctgtc
     5101 cacgctgacg catcgcgcct cggtcaagtg ttcgtgaatc tgttgctcaa cgctgcgcaa
     5161 gcgatcgtcg aaggcgatgc cgatcgcaac gagatcaggg tcagtgtgcg cacgagtgac
     5221 gatggcacgc gcgtccgcgt cgacatcgca gataccggta cgggcatcgc gccatcgatc
     5281 atcgggcgca tcttcgatcc gttcttcacg acgaaggctc ccggcgccgg caccggactt
     5341 ggcctcgcga tcagccatca gatcgtgcgt tcgatgggtg gcgacatcac tgtagcgagt
     5401 cagctgggca agggcagcac gttcagcgtg gtgatgccgg tcgccgagcc cgcctccgcc
     5461 gacgccgaag acacatgcgc cggcaccgcc agcgtcaacc gcaggatcct tctcgtcgat
     5521 gacgaggccg cggtcggcag gtcgttgcag ctgctcctcg ccccagaaac cgaggtcatc
     5581 gcggtgttgt ccggcgagca ggcgctggcc cggctcaccg caggcgagca cttcgacgcg
     5641 atcgtctgcg acatcatgat gccgcagagc accggcatgc agctctacga cgacgtcgcg
     5701 cgcgttgcgc ccgaatatcg cagccgcatg atcttcatga ccggcggtgc gttcacgccg
     5761 caagcgcgcg cgttcctcac gaagctcgat cgcccgtacc tcgagaagcc attctccgag
     5821 caggagctgc gtgacgcgct cgcccgcgtc aaagcctgac aatcgtcaga ccagttgctg
     5881 ctaggatggg ttccaatggc actccgtgtg gccggcgtgg tgctcgcatt agcggcgacc
     5941 gcgagcgccg accggctgtc ccccgtcgat ctcgcgcgta agaacgacgg cggctacgtc
     6001 accgggctac cgctggtgtc gtactcgacg gacctcgggt tcggtggagg cgcgcgcgcc
     6061 tactactact ggaacggcca ccgcgacgac gcgaggttcg cgaacacacc gtacctgttc
     6121 cgcgtgttcc ttcaggcgta cgcgacgacg ggtggcttgc agttccactg gctcgatctc
     6181 gatgccccga ggttcgacag ctctccgtac cgcatacgca gccagctgat ctttgcgcgc
     6241 aatacgaaca gcaattactt cggtcacggt gcgcgcagcc tcgacccgtt gcgcttcccg
     6301 gggtccgccg cattcgacag ctatgccgac tacgccgccg cgcaacatca gatcatgggt
     6361 ggcgagacct tcgcaaagta cgatcagtac gacctgctcg agccgaacgg catcgcgatc
     6421 atcgagcgtg gtttcgacaa cgaccgcgtg cgcgtgctcg cagggctcgg cttctcgtac
     6481 gcgcgtatcc acgactacac aggtaagacg gtcgatgcaa cgagcggcca ggcgaccgag
     6541 gcccagaccc gccttaccac cgactgcgcg gcgcagcttc tcgtcggctg cagcggcggc
     6601 cgcgatgacc acctgcgcgt cggtgtctcg tacgacaccc gagacttcga gcccgacccg
     6661 aatcgcggcg tttttctcga cgcggtgctc gacgtcggca cggccgcgct tggctccgag
     6721 tacgactacg tccgattcct cgtcgcggcc cgcggctact actcgccgat ccccgaccgt
     6781 accgacctcg tgctcgcgac gcgcgcggca ttcgagtggc aatcgtccgg aaccccgttc
     6841 ttctcgatgg acacgatccc gttcaccgaa gatccgcgtc agggcttggg cggccaccgt
     6901 acgctgcgcg gctaccgtca ggaccgcttc gtcgggccgg tgatggcgct cgtcaaccag
     6961 gaagcccggt ggacgttcgc gcgcacgacg ctgtggcgcc agaagctcgc tttcatcgcg
     7021 gtcggttttc tcgacgtcgg ccgcccgttc gaccgcgtgt ccgagctgtc gctccgacgc
     7081 tggcggcctt cgtatggtgg tgcgatgcgg atctcgtgga acctggcgac ggtgatcacc
     7141 gtcgactacg gtgtgtcgcc agaggacagc ggcctgtaca tcaacttcaa ccacatcttc
     7201 tgagcggcga tcgctacctg gcgcaagcgc cggcaaagac cttcgcaacc gtcgcggcat
     7261 ctggccgcgc tttcgcgtcg atctgcaagc atgcgtcgac gatcgcggcg acgtccgctg
     7321 gcagtccggg cacaaccgat gcgagaggtt gagctggatc aggcatcggt ttaccggcaa
     7381 ggaacgcttt gacgacgggg cgatcgtacg gccgctgtcc gctcagcatc tcgtacgcga
     7441 cgatgccgaa cgagaacaga tccgagcgcg gcgacgcgtg atccgcgccc tgcccgagct
     7501 cgggcgccat gtagcgcggc gagccgatca acatgcccac cttggtcaag ctcgggttca
     7561 gcgtcgcggt ctcgtcagga tcgggttccg cttcgacgac ggtctgggct tcagcgcgct
     7621 cgggatcatc cgggcggcca acgtgcgcga ctccgaagtc ggtcagcttg aggacgtcac
     7681 ccgccacgag gacattggcc ggcttgatgt cgcggtggat gacgccgagc gcgtggatgg
     7741 tttcgatcgc cttggcgagt tgcgcgacca tcggcacagc ccagctcaga tcgccgaacc
     7801 ggtcacgtac gtgctcgagc gagttgccct cgatcagctc cagcacgagg aacagcacag
     7861 cctggtcggt gatcccgaag tcgaggacgc tgaccacgtt cggatgatcg atgcgggcga
     7921 ccagctccgc ctcgcgcgcg aatcgggcaa gctttcccgc gctgcctccg cgcacgagct
     7981 tgatcgcgac ggcgcggccg tcggagagcc gttcggcgcg atacaccttg cccatgccgc
     8041 cttcgccgag cagctgatcg atccggtact tctcggcgag cacggtaccg gggtcgagct
     8101 cttgtgccga ccgtttcggc gacgccatca gcgtcagcgc gtgcgacagc tcgtgcgatc
     8161 gattcgcgat ctgctgacgt agctcctcgt tcagtagctg cacatcgcgg ttgcgttgct
     8221 cgagctcgcg actgcgccgg cggttgagga atgcgagcag cgtcgactgg cacagcacga
     8281 atgccgtcag gccgtactgc gtagtcgcct gcagtggctg cacgacacgg cgcacccgaa
     8341 ccacatccca cgcgacggcc aacgcgagca gcacgaaccc tacgagcatc agcacagaag
     8401 gcaggtcgcg cttaccaatc acagcgcgcg cgaggctgac gatcgcgtac gcgagcgctg
     8461 cgaagatcag catctggtac gcggtttgga gaagcgtgaa gatcggggct ggcagcgcga
     8521 ccgtcagcgc aaatacgagg ctcgccgcga caaacccgcg aacggcgagc gcgtgcacgt
     8581 acgtggggaa caccgcacgg atgaacagcg cgatgagcgg tgacccgagg tagccggtca
     8641 gatactcgag gcgcaacgac attactgtcg agaacccggg cacctcgtcg gccggatacc
     8701 gcccgaggat aaacacgcgg cccgcgatca cgagacacac gaggccaaac cacagcgacg
     8761 cgcgatcggc tcggttcatc acgaattgca cgacctgctg cacgccgacg aggatcaaca
     8821 ggccgagcac gaagaaatcg gtcatcctgc gatgccgctc gcgttggccg atcgccgcgg
     8881 cggtgcccag agttggcgcc ggccccgagc cgccgcgcgc gtgctcgaag ttgctgacct
     8941 gcaacgtgag caacacgtcg ccgccggcag tgaacgatac gactgcgttg cgcggcgtcc
     9001 cttgcgattg atcggatgtg gtccccaccg ttcccgagcg cagcggctcg gctaacagct
     9061 gaccatcgag gcgtgacacg acaaaccgat tcgcatcgac caccggagcg gtcgcgagcg
     9121 cgtattgctt gggcgtctcg ggaaggtgca cgatcaagcg atacgtcgcg aagccatctc
     9181 caccgagctc gcggccgtca gtgagcttct ggccgttcca ccgtccgatc gggagcacac
     9241 catcgggcgc agtgttgtcg cccggctcga tcagcttctt ccagtagaac gtccagccgc
     9301 cctgtagcga cagcacgctc gtgtcgtcga gctcccagcc gcgcagatcg aggacaccgt
     9361 cgactgctcg aggcgccgag ctgtcatcgc agccgagcac gacgagtagg ctcaagaggg
     9421 cgagcgcgcg agccacacgc ccatcgtaac gggtttggcg gagtagctcg tgtcgaacgt
     9481 cctctcaggc acgcgaagaa gctgcgtccg tgccgaattc gggcgcttcg ctgagcttcg
     9541 gcaacgcaat gatcgcaggc acgattacga atgctgcaac gacacatgtg agctcgccga
     9601 tcagcgacgc caggccgaag ccgcggatcg acaggttgtc gctgaccagc aacgacatgt
     9661 agccgatcac ggtggtcagt gagcacacga gcaccgtgcc gcccgtcgaa cgcagcgcga
     9721 tcatcgggtc gccgtgcgcc gcgcggtccg cgatgttgat cgcgtagtcg atgccgagac
     9781 cgagcgtgat cggcagtgcg acgaaatcga ggaagttgat cttgagcccg gcgagcgcgc
     9841 acaccgcgat catgccgatg ctgcccgcgc tcgatgccac gagcaccgcg accgcgcggc
     9901 gcgagcggcc gacgatcagc acgaccatcg cgatcagcgc gatcgcggca attgccgtga
     9961 cgagcgggcc gtccttctgg atctgcgtga gcacgtccgc gaacaacatg catgcgcccg
    10021 acgcggtgac gtcgtcgtgc gagagcttgc gcaccgcgcc cgagaagcga atcaggtcgc
    10081 ggccgtcgcg ctcgtcgaac tcggcgctcg gctttaccgc gatcatgagg ccgacgcggc
    10141 cgtcacgttc ggtcagcttc cgcgcgagcg cgggcggcag gtcttgcgcg gtcaccgcgg
    10201 ccagcgtgtc ggccggacgc agcgcgagga gctcggcgcg ttgctgttcg tcgagctgcg
    10261 gcgcgatctc gtcgatctgg tcgcgaatct cggcgaggag cgccagcttc gcggcttggt
    10321 cctgcggaat gacgtcgagc gccgagctca cgctgccgac catcggctcc tgcttcgcga
    10381 tcgcgtgcag ttgcgccacc acgctcggca cctgctcctt gcgatcgacc gcgacgaacg
    10441 tctggccggc aagcccggcg aggccgcggc cgaactcgtc gtcagagtac ttcagccagt
    10501 cgcgcgtcgc gcgcgcatcg ggcgcctgcg agcgcagttg cgtgaggtcg tactcgtagg
    10561 gatcgtgggt gaggtagtgc cacgagatca cgcacgcgcc gatcgtgatc gccgccgcga
    10621 cgccggtgac gagcacaggg cggcggaagc cgaacagctt gacgacgatg cggccgaaca
    10681 tcggcgacgg ctcgcgcatg ctgttgccgg caaacctcat gatcatcgcc ggcagcagca
    10741 cgaacgagca gatccagcac acgatcatgc cgacgccgcc gatcagcgcg aagtcggcga
    10801 agccgcggaa cttcgtcgcg ccgagcgccc cgtacgcgat cgcagcaccg agcgacgcga
    10861 cgagcgtcgg catcagcgtg ccgcggatcg cggtcgtcat cgcctgcgtc acgttcgccg
    10921 tccggcgctc ctcgaggtag cgcgcgatga gcaggatgcc gtagttgaca ccgttgcccg
    10981 cgatgatcgc gccgaggaat gcagtcgctg cattgaggtg accgacggtg agcgcggcgg
    11041 caccaaacgc gatcagcgtc gcgatcaaga tgttgaaccc gagcagcgcg agcgcgcgct
    11101 tgctgcgcag gtgcaggaac agcacgagcg acacgagcaa gccggtgagc aagctcgaca
    11161 tcacaatgcc gcgtgacagc gcgccgtgct cggcgagcga caccgccgga ccgccggcaa
    11221 accccaccgt cacgaacgga tgcgcgccgc ggattttcat cgcgaccttg tcgagcgcgc
    11281 ccatcagctt gcgatcgcgg tcgacgtcgg tcgcgacaaa cgcggtatgg acgacgatca
    11341 cctgcgagcg gccgtccgcg ctgacgtggc ggtcgcgcgc gagctgcgca tcggcgtcct
    11401 tctgtttctt gcgcagctcg tcgagctcgt gggtgtcctg cggcgccggc tcgtcatcga
    11461 tatcgatgaa cagcgggttc gcgtgcagct tggcatccgc gatctgcttc gcgagtgcat
    11521 cgcgcaccgc ggtcagctcg ttcatcggta cgtagagctc gcgatgctcg cgaatgaacg
    11581 cgcgcgtcgc gacatcgtcg ccatccgcgc tctcgacgag ctcggggccg agcgcggaaa
    11641 ttccggcaag cgcctccgtc gctgccgcct cgcgcgactt cggatccggc gcgacgatga
    11701 cgacgagcat cgtgtcgcgc gacggggcgc gcttcgcgag cttctcggcc gccttcacgg
    11761 acggcgtgtc acccggcaac agcgacgaga agtccgtctg cagcggcagg tggaacgcag
    11821 cgagatagat cgcgatgcca acgagcacga ggctcgacgt gaagatggcg accgtatgcc
    11881 gctggaccca ctggacgtac cgctgcgtgc gcatttgcgc gagataaggc aaccggcatg
    11941 ccggggccta acccctgcag aacctggcaa atccggtcaa agctgtcgaa ccctcgacgc
    12001 aggacgttga gcgcccggcg ctgccgcgtc gaacagctgc ccggacccgg tcgggttagc
    12061 ggcggccgat cgcggctggg ttgtagcgct cgtaggtggc ctggcccacg gtgccgccgg
    12121 agatcaacac cgtgccgtcg cacagcggcg tcgcctggtg attcatgcgc ggcacggcga
    12181 ggttgtcggt ggcgacgacc cggagttggc cgtcgagcgg atcgaggctc gcgatgaacg
    12241 cggtgtcgac cggcggcccc ggtttgccgt ccgcatcgac cgtgatcgcg ccaccggtga
    12301 tgagcacgcg gccatcggga agcacggtcg cggacatgcc gatccgtcca gcagtcggcg
    12361 ctttttccga caggagaccg accgaaatga accggccgtc gagcggcgag aacagctcga
    12421 gctgatccgc gatctcggtc tggcccgtca tcgggttgat cgtcagacca ccgatgaaca
    12481 agacgctgcc atcgggcaag cgcgccacct ggtgattcca gcgcggcacg atcatctgcg
    12541 cggtgaactg atcggagaag ttgtcgaaca acggcttgta cagctcggct tccttgatcg
    12601 gctttccgtt cgcatcgagg ccgccagcaa cgagcacggc cgcaccgagc tcgtcggtga
    12661 ggcgcgtcgc ggtgtgggag taccgcggat acttgagctg cgcgcgcagc gcagtgatcg
    12721 cggttgtacc ggtgtcaacc gacacctggg cgacgatgtt cgaaggaccg cccgcgatcg
    12781 ctttggccgg gtcaggtacc tggtagccgc cgatcacgac tgcgcggcca tcgcttagtg
    12841 ttgtcgcggt gagccgcgtg cgattgagcc ttggctcgaa gaacttctcg tactggcggt
    12901 cagtgttgcg ctcggcctcg atgacctccg cgaactctgc gccgacgcca gtggctgggt
    12961 cgagtccgcc gacgatcacc atgcgcgagt cggcaccggt gccgacgatc gcgaccgcag
    13021 cgtcgtagcg gcgctcgata tcgtgcaggt tgtcgtacgc gcccgtgctc ggatcgaagc
    13081 gctcgatctg ggtcagcgcg agcggtttgt tgttctcgtc gggatactcg ccaccgacga
    13141 gcaaccccga gccgtcgcga tacatcatcg cctggccacc aatccgcggc agcggctgct
    13201 gtgacatctc gccgaacttg acctggcgcg cgaagaacaa gtgcggcgtc ggcagacgcg
    13261 cgtcaggacg caccgagaac tcgcacgtgc ggccgtaggc gtactcgctg ttgcttctcc
    13321 ggccgaccat gtggacgacg aggtcgtcgc cgtacggcac gttcggcagc tcgatgacgt
    13381 cgccatgcga gaacgtcgct gacgtgatgt catcggcggc gccagcgtgc gcgatcgaca
    13441 gcgtgatcgt gtcgaggtcg agcggctgtg catcgtcatt ggcaggaatg tcgatgatcg
    13501 gcgtgaccac gaccgtgttg tcagtgcagc cgacgaggac gaccagtgcg agcgcgtgcc
    13561 tcatagcggc acgtacctgt cgggccagtc cgggcgcgcg gtgaatgcgg gcgagccgct
    13621 gtcttgcgtc gcgaagatcg tcaacggaat cacgatcgcg gccgcgccga ctgcgccacc
    13681 gaccgcccat gcccacttgc tcttgtacca ggggcgatgt gcccgcggcg tcagcagcgc
    13741 gctgaccgca tccgcgagcg gctcgaggtc ggtgccgatc gtgaccgtct tgcgatcgcg
    13801 ttccttgccg tcgaggccga ccagccgcgc ggtcgcgacg ttattgcgca cctcggcgac
    13861 gatgaatgcg cgcgcgccgc tgcgctgcgc ttgaccgagc agctcggtat cggtcggtgc
    13921 cgcgaactcg ggcggcgtga tcggtagcgt gatcttgccg ccgaccagct cgaccctgcg
    13981 accgtcgggc tcgcgatcgg gacacgtcac gcgcacccag tggcggccga cgatcaacgg
    14041 aatcgcagtt gcgacgttga cgttcgttgc gtcgaccgtc gcgcggcagc cttgcggaac
    14101 cgtcagcgtc agctctgcgc cgcctttgtt cttgacggtc gcctgtgcgc gctcgaactc
    14161 ggcaatgatc tttggtggga agcgaccggg gtcgagctcg cgggtggcgt cgaccgtgtt
    14221 cgcgacgatc agctcctccc acgcgccgtt cgaatcgccc tgctgcgtct tgatcagccc
    14281 ccggtacaag aacaagtcgg agagctgcgc ctgtgtgaga ccggcagccc cggtccggtc
    14341 gacctccgag cgcgcgctat cgagctgctg ccaggcctgg tcgaactcga gtcgctcgaa
    14401 ggcgtcgagt cccttctgga ccagctgcgc ggtggtggcc gtaccctccg gttttggcga
    14461 acgatccagg atggctgcac ctgccccgcg ggcggcctga accaccggcg cggtgcgcaa
    14521 acccggtgcc cagaccacaa cgaggtcagc cgtcggcgcc gcgtcggcgc gagccaccag
    14581 cgccaggacg accaagaggc ccagagtgcg cactcaaatg tgagtttcgg cctgttccga
    14641 tgaaccgtca accgcacgct ataatcgccg ggcatgagcc gtgcgctagg tcgctacgag
    14701 ctcctccgcc cgttagcgcg cggcggcatg ggcgaggtgt atctggcgcg tcgacgagct
    14761 gcgggcgtcg agaagtggtt ggtcgtcaaa cggatgcggc ccgagcgcgc cggcgacgcg
    14821 cggtttctgg acttatttat gcgcgaagcc cggctatcga tgagcctcgc gcaccagaac
    14881 atcgtgccgg tcttcgactt tggccgcatc gacgaccagg tattcctcgc aatggagcgt
    14941 gtcgagggca aagacctcgg ctcgagcatg acgaaggccg gcaaggggat gccgccgctt
    15001 ttggcggcgt tcatcgccgc agaatgctgc caggcgctcg actatgccca tcgccgcaaa
    15061 ggtcccgacg gcgccgacct cggcatcgtt caccgcgacg tcaccccgcg caacgtgctg
    15121 ctctcgtggt cgggcgaggt caagctgacc gattttggta tcgccgctct ggccggcgac
    15181 gagccgagca agatggccgg cacgccggca tacatggcgc ccgagcaagc gcgcagcgag
    15241 ccgaccgacg tccgcgcgga tatctacgcc gtcggcatga tcctgcgcga agcgctgacc
    15301 ggtgcgcgtg gccgccatgg caacgatcgc gaaaccgtgc tcgccgcggc gcaggccggc
    15361 aagctcgacc cgtggcccga gggcttcgac tcgccgctga aggcagtcgt cgatcgcgcg
    15421 accgcgaccg cgaaagagga tcgctatccg gacgcgcgcg cgatgctcga ggatctcgac
    15481 gacttcatcg tcgccgagcg cgctgcgaag aaagccgaag caccggcgcg ccagctcgcc
    15541 gcgtggctcg ccgaggtgtg gcaaggcgag cgcgacgacg tcacggtcag cgacgtcgac
    15601 atcgaagcga agcacatgat gagcttcctc gacgacggtc aattcgacgt ggtcggcacc
    15661 ggcacgatgc gctcgatggc gatgacggcg gccgaggacg tgctgagcaa gcacagcgag
    15721 gtgaaagccg acgcgacgcc ggccgaggtc acgccgtcgc cgcgagcgcc aaccgctggc
    15781 gagcgccccg acacggcgac accggcgcca cagcggcgat cgaagttgcc gtggatcttg
    15841 ctcgttgcgg tcgtggtcgc cgcgagcgcg atcgtcgtcg cgatgtcgat gaacaagccc
    15901 gagcaaccgg agcgtgtcgt cgagccgccc atcgcgaagg tcgaagcaga cgcgcacgag
    15961 ccgatcgtcg agcccgaagc ggtcgatgcg gtcgtcgcgc cacccgccga tgccggcgtt
    16021 gccgatgcac ccgcgcgcgt gaagccgcac aacacgcaac atgtcgtgac gcctcgcccc
    16081 gacgctcacg ttgcggcgcc gcccgcctcg gcgacgaagt acaaggtgac gatcagtacg
    16141 ctgccgaagt ggtcgaactt cactgtcgac aacgatccga cggtgtatca gagcttcgac
    16201 tcgatccagc tcgcgccggg accgcacaag gtccacttca ccggcagcgt gcactacccg
    16261 gccgacaaga tcgtcacgat cacggtccca gagaaagact tcaagacgac cgtgacgctc
    16321 gatgctcccg agaaggatgc tccggctccg tagtcaccgc gatgttctcc gcggccggag
    16381 gtaacgcgac cgtgccgtcg tcggcgatcg tggtcactgc cggcagcttg ccgacgtcgc
    16441 cggtgatcag ccgtgactcg cggcggtagg tcaaccacga ccacgcccag tggaacatca
    16501 tgaacaggcg gttgcggaaa ccgacgagga agaagatgtg gacgacccac cacagcatcc
    16561 acgcaagcag gccgtgtttc gcgaatcgct tcgtcgcgat cacggcatcg gcgcgaccga
    16621 tcgtcgccat gttgcccttg tcgcggtagt ggaacgcaga ccgcacgccc tcgccgcgcg
    16681 cttcgctatc gatgatcttc gcgacgtgct tgccgctctg cagcgcgccc tgtgcgacgc
    16741 ctggcacttc gacgccgtcg ctcatcatct tcgcgaggtc gccgatcacg aacacttcgg
    16801 ggtggccggg aatcgacagg tccgggttca cgagcacgcg gcccgtgcga tcgagcggcg
    16861 cgccgagact tttcgcgagc ggcgacgctt gcacgccggc ggcccacaac acggtacgcg
    16921 tgccgatccg ctcctcgccc gcaggaccct tcacggtgat cgaccgctcg tcgatcgacg
    16981 tgaccatcgt gttgagtcgc accgacacct ggcgcttctc gagtgattgc ttcgccgcct
    17041 ccgatagttt ttcggggtag gcgttgagca gccggtcgcg tccctcgaac agcagcacgc
    17101 gcaagctcgt cggatcgatc gagcgaaaat ccttcgcgag cgtgtgcagg ccgatctcgc
    17161 cgagcgcgcc cgcaagctcg acgcccgtcg gaccaccgcc gacgaccgcg aacgtcagcc
    17221 actcgcgttc tcgctcgagg tccgactcgc gctccgcggc ctcgtaggcg acgaggaggc
    17281 ggcggcggat ctcgagcgcg tcctcgatcg acttgagacc aggggcccgc gcctcccact
    17341 cgttcttccc gaagtacgag tgcgtcgcgc ccgtggcgac gacgaggaaa tcgtagtgca
    17401 gtgagccggc atcgagcgtg accgtgcgct tgtcgacgtc gatcgacttc acgtccgcca
    17461 gcagcacgcg cgcgttcttc tgacgcgcga gcacgctacg aatcggata
//
LOCUS       DASUFX010000365         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_752455,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000365 DASUFX010000000
VERSION     DASUFX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752455"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VFV99_14570"
     CDS             <1..1657
                     /locus_tag="VFV99_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5060588.1"
                     /translation="VASAACCSKSEVKGGGSGADVPPPAKPSFTVFALAETRGQIGPC
                     GCTSDPLGDISRTAKLVEEARAKGPVLVVDAGSLLYGQNPVPKHLEAQEDLKADLLTT
                     IYKDQLQVAAVGLGPADLAKGPAEIRFPRVAVNVVDPAVKLQPASVINVGNVKVGVFG
                     VVATDAIKDVKLDDPVAAGKRAVDTLKKAGAQSVIALVQATSKRDAVAMVRDIGGIDI
                     AIAGLGAVAPEPDRIDVEPTKVGDGWLVIPANRGQIVSRLDVYIHGGGGFVDAVGRGA
                     AAAKIAQLDHQIASLDAELAKFKQDKDADPKFVAAKQSERDAAAAQKKKLEAQPLEAP
                     AKGNYFTLEQLRINKTLACSVPVRDAIRGYDLAAGEANVKAAADTPVPAPKKGQAGYV
                     GTEACSDCHQDQVDQWKTTRHASAWKTLVDRGQQFDYECIGCHVTGWQQPGGSNLAHN
                     DNLRDVQCETCHGPGSIHVAKGGLEKPFAIVRNPKPELCASQCHTKEHSDTFQYEAYL
                     RDIVGQGHGAEMRTKLGDGPTGHELRSTALDKAGRTLGAGCVK"
     gene            1683..2075
                     /locus_tag="VFV99_14575"
     CDS             1683..2075
                     /locus_tag="VFV99_14575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5060589.1"
                     /translation="MTRSVRTWLVAIALAGAACGGGQKPKKSSGQSYGVQSGLGTWYG
                     SEQHGGPTASGERFNKNAMTAAHRSLPMGTRVRVTNERNGRSVIVRINDRGPYGSKRR
                     IIDVSEAAARQLGMIDAGVVPVRVEVVK"
     gene            2139..3074
                     /locus_tag="VFV99_14580"
     CDS             2139..3074
                     /locus_tag="VFV99_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5060590.1"
                     /translation="MSDAPPLIEADKLTKRYGSIVAISNVTAQVRGTIIGLLGPNGAG
                     KSTLLKSLLGLIPFEGGAKVLGLDAKTHDREIRDRVGYMPEQEALLAGMSAVELCTYA
                     GELSGLPRTEAMQRAHAALYYAGLEEKRYLPIDGYSTGMKQRVKLAQALVHDPEILFL
                     DEPTNGLDPRSREEMLHLITELPTRRGCAIVLSTHLLPDVERVCDSAVIMHQGRVRFV
                     GTIEELRGARGLDTSITVEVKANAPKLAEVLGAAGATCKVKSPVAVEVDLPTNATSML
                     VFQRAKEAGLQVRGIEVRRESVENAFLRVIGEVAP"
     gene            3071..4027
                     /locus_tag="VFV99_14585"
     CDS             3071..4027
                     /locus_tag="VFV99_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060591.1"
                     /translation="MSEPAAPGGPPYRTPADAPKPAGTIYDTGYKRYVGTRRSVATRW
                     LVIMRHQIAMAWKKWWRYKMPLFGAVVTTFVAAGLLYFRTSKIMRQVGTAGGGEILRM
                     IDAVVPLSFEWYCRAAFMLTLTLGATIVATDTQSGAFTFYYVRSIRPMDYVLGKLAGV
                     GILIATLVMLPPLVLVGMRIGFSADTAEVTEQLRLLPKMLALGGLATLVYTAVPLAMS
                     SLVANRRYALALWVAYYLIVGFIAGQISMFVTADAGVLDIQTSLQAITYELFDMKVMR
                     IRTGGMSPKLALFGLLAQSAFAIAILWFQVSRDQKTGVGGSS"
BASE COUNT          713 a         1277 c         1417 g          636 t
ORIGIN      
        1 ggtcgcaagc gcggcttgtt gctcgaaatc agaggtaaaa ggtggcggat ctggtgccga
       61 cgtcccgccg cccgcgaaac cgagctttac ggtattcgcg ctggccgaga cccgcgggca
      121 gatcggaccg tgcggctgca cctccgatcc gctcggagat atctcgcgga cggcgaagct
      181 ggtggaggag gcccgggcga agggtccggt gctcgtggtc gatgccgggt ccctgctcta
      241 cggccaaaac ccagtcccca agcacctcga ggcgcaggaa gacctgaagg ccgacctgct
      301 caccacgatc tacaaggacc agctccaggt cgccgcggtg ggcttgggtc ccgcggacct
      361 cgcgaaaggc ccggcggaga tccggttccc gcgcgtcgcg gtgaacgtcg tggacccagc
      421 ggtgaagctg cagcccgcga gcgtgatcaa tgtgggcaat gtaaaggtcg gcgtgttcgg
      481 cgtggtcgcg accgacgcga tcaaagacgt caagctcgat gatccggtcg ccgctggcaa
      541 gcgcgcggtc gacaccctca agaaagccgg tgcacagtcc gtgatcgcgc tcgtgcaggc
      601 gacgagcaag cgcgatgcag ttgcgatggt ccgcgacatc ggcggcatcg acatagcgat
      661 cgccggtctc ggtgccgtcg cgcccgagcc ggatcgcatc gatgtcgagc cgacgaaggt
      721 cggtgacggc tggctcgtga ttcccgcgaa ccgcggccag atcgtgagcc gcctcgatgt
      781 ctacattcac ggtggcggcg ggtttgtcga tgccgtcggt cgcggcgcag cggcggcgaa
      841 gatcgcgcag ctcgatcatc agatcgcatc gctcgacgcc gagctcgcga agttcaagca
      901 ggacaaggac gccgatccga aattcgtcgc cgcgaagcaa agtgagcgcg atgcggcggc
      961 agcgcagaag aagaagctcg aggctcagcc gctggaagcg ccggcgaagg gtaactactt
     1021 cacgctcgag cagctgagga tcaacaagac gctcgcgtgc agcgtgccgg tgcgcgacgc
     1081 gattcgtggc tacgatttag cggccggcga ggcgaacgtg aaagccgcgg cggacacacc
     1141 ggtgccggcc cctaagaagg gccaggccgg ctacgttggc accgaggcgt gcagcgactg
     1201 ccaccaggat caagtcgatc aatggaagac gacgcgtcac gcgagcgcgt ggaagacgct
     1261 cgtcgatcgc ggccagcagt tcgattacga gtgcatcggc tgccacgtca ctggctggca
     1321 gcagccaggc ggctcgaacc tcgcgcacaa cgacaacctg cgcgacgtcc agtgcgagac
     1381 ctgccacggc cccggctcga tccacgtcgc gaagggcggg ctcgagaagc cgttcgcgat
     1441 cgtgcgcaat cccaagcccg agctgtgtgc gtcgcagtgc cacacgaaag agcacagcga
     1501 cacgttccag tacgaggcgt acctacgaga tatcgtcggc caaggtcacg gcgccgagat
     1561 gcgcacgaag ctcggtgacg gccccacggg ccacgagttg cgatctaccg cactcgacaa
     1621 ggcaggccgc acgctcggcg cagggtgcgt gaagtagcgg ctgcgtgcat aatcgatcgg
     1681 gcgtgacccg ctcggtgagg acgtggctcg tagcaatcgc gctcgcgggt gccgcgtgcg
     1741 gtggtgggca gaagccgaag aagtcgagcg gccagtcgta cggcgtgcag agcgggctcg
     1801 gcacctggta cggcagcgag caacacggcg ggccgaccgc gtcgggtgag cgattcaaca
     1861 agaacgcaat gacggcagcg catcgctcgc tgccaatggg cacacgtgtg cgcgtgacga
     1921 acgagcgcaa cgggcgcagt gtgatcgtgc ggatcaacga tcgcgggccc tacggcagca
     1981 agcgccgcat catcgacgtg tcggaggccg cggcgcgcca gctcggcatg atcgacgccg
     2041 gcgtcgttcc ggtacgcgtc gaagtggtca agtgatcgcg ggcacgcgtt ccgccgtgac
     2101 cgcgtgatcg gtgtgtgcta cagccgtcgt gccagccgat gagcgacgcg cccccgttga
     2161 tcgaagcgga caagctgacg aagcgctacg gctcgatcgt cgcgatcagc aatgtcaccg
     2221 cgcaggtgcg tggcacgatc atcggcctgc tcggccccaa cggcgcgggc aaatcgacgc
     2281 tgctgaagtc gctgctcggg ctgattccat tcgaaggcgg cgcgaaggtg ctcggtctcg
     2341 acgcgaagac gcacgaccgc gagattcgcg atcgcgtcgg ctacatgccg gagcaggaag
     2401 ccttgctcgc gggaatgagc gcggtcgagc tatgcacgta cgctggcgag ctctcgggcc
     2461 tgccgcgtac cgaggcgatg cagcgcgcgc acgcggcgct ctactacgcg gggctcgagg
     2521 agaagcgcta cctgccgatc gacggctact cgacgggcat gaagcagcgg gtgaagctcg
     2581 cgcaggcgct cgtccacgat cccgagatct tgtttctgga cgagccgacg aacggcctcg
     2641 atccgcgctc gcgcgaggag atgctgcacc tgatcacgga gctgccgaca cgccgcggct
     2701 gcgcgatcgt gctgtcgacg cacctcttgc ccgacgtcga gcgcgtctgc gatagcgccg
     2761 tgatcatgca tcagggtcgc gtgcggttcg tcggcacgat cgaggagctg cgcggcgcac
     2821 gtggcctcga cacgagtatt acggtcgagg tgaaggcgaa tgcgccgaag cttgccgaag
     2881 tgctcggcgc ggctggcgcg acgtgcaagg tgaagtcgcc ggtcgccgtc gaggtcgacc
     2941 tgcccacgaa cgcgacgagc atgctcgtgt tccagcgcgc gaaggaagcc ggcctgcagg
     3001 tacgcggcat cgaggtgcgg cgcgagagcg tcgagaacgc gttcttgcgc gtgatcggcg
     3061 aggtggcgcc gtgagcgagc ctgcagcacc cggtggtccg ccgtatcgca cgcccgcgga
     3121 cgcgccgaag cccgccggca cgatctacga cactggttac aagcggtacg tcggcacgcg
     3181 ccgctcggtt gccacgcgct ggctcgtgat catgcggcac cagatcgcga tggcgtggaa
     3241 gaagtggtgg cgctacaaga tgccgctgtt cggcgcggtc gtgacgacgt tcgttgcggc
     3301 ggggctcttg tacttccgca cgtcgaagat catgcgccag gtcggcaccg caggcggcgg
     3361 tgagatcctg agaatgatcg acgctgtcgt gccgctgtcg ttcgagtggt actgccgcgc
     3421 tgcgttcatg ctgacgctga cgctgggtgc gacgattgtc gcgaccgaca cgcagagcgg
     3481 cgcgttcacg ttctattacg tgcggtcgat ccgcccgatg gactacgtgc tcggcaagct
     3541 cgctggcgtc ggtatcctga tcgcgacgct cgtgatgctg ccgccactcg tgctcgtcgg
     3601 catgcgcatc gggttctcgg ccgacacggc ggaggtcacg gagcagctgc ggctgctccc
     3661 gaagatgctc gcgctcggcg gcctcgcgac gctcgtctac acggcagtgc cgctcgcgat
     3721 gtcatcgctc gtcgcgaatc gccgctacgc gctcgcgctc tgggtcgcgt actacctgat
     3781 cgtcgggttc atcgccggcc agatatcgat gttcgtgacc gccgatgccg gcgtgctcga
     3841 catccagacc tcgctgcagg cgatcaccta cgagctattc gacatgaagg tgatgcggat
     3901 ccgcaccggc ggcatgtcac cgaagcttgc gctgttcggc ctgctcgcgc agagcgcgtt
     3961 cgcgatcgcg atcctgtggt tccaggtgtc gcgcgatcag aaaaccggcg tcggaggctc
     4021 gtcgtgagca tcgtcgtcgc cga
//
LOCUS       DASUFX010000366        17099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1170267, whole genome shotgun sequence.
ACCESSION   DASUFX010000366 DASUFX010000000
VERSION     DASUFX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170267"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFV99_14590"
     CDS             complement(<1..906)
                     /locus_tag="VFV99_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060592.1"
                     /translation="MAETSDPKTLAGATDGVTKAAQLPAHFPGWAAKLAELYFSGTTS
                     MFVLHGNTFDVVRAGAENDPRWTGLADFIADQLFGRWDLVVHYDLARGLRCAAGTSSK
                     RLQEMVATANKWIGDLRQLPRDPTKGLLALDLMIQKIVMADDNQRIRCAVIIDHVGYV
                     APSGDNLDLNEQTHLVTLLNWASSPYIKRLNMAFVLIDPRLSSVAERLSSNPHVAAIE
                     VPLPNEAERTAFLAAQVKATGKDVASFSDYQVPELGKLTAGIGLTDLEVLVRSAAEGT
                     RRLDRQYFKELKKRLIERQAQGMLEF"
     gene            complement(906..1679)
                     /locus_tag="VFV99_14595"
     CDS             complement(906..1679)
                     /locus_tag="VFV99_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060593.1"
                     /translation="MILTKFFKAIAAQFNKLGNWLTNKDPIAQMQYEYDKSVEQLKEG
                     REGLEQYRGLVERVTRQVKDGEAQVAKLTAQAKAYLKLNDRDKASIIALQLTKVKTQL
                     GENQAQLAMHEEAYNNNLKKIQFANKKLAEVKDKIQKYDADLKMSAAEAEIAKLTETF
                     NMDLTTDFGQMEDAIQRKIDANRGKARVAADMSRQGMEKIEDDERLEKQMAEDALKGL
                     EVDLGMRAPETTPVQQAQKDLGPAEAEAARKELENLEKS"
     gene            1906..2670
                     /locus_tag="VFV99_14600"
     CDS             1906..2670
                     /locus_tag="VFV99_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060594.1"
                     /translation="MADKNPSTALALVNPVGLGVAAGAATVAIALTNPLIGVVGGGVW
                     LATVAIDAIRRSRKKKKRAAVSIPMMKDPDDIVDPTTRAAVQKILANKAELQKILDET
                     PADLQIHISKTLGSLDELEMYAVRLVERAEDITTHLSKMNLPALVAEVKQLATRAAAA
                     KDPVTRQSYEEAKQARMDEIRSLKEIKTTKDRIDANLMRVVAVMGALPTKVVHMRALD
                     AQALDQLSGDISHDLSAIGEELKTSEAVIKQQLVAQ"
     gene            2679..3314
                     /locus_tag="VFV99_14605"
     CDS             2679..3314
                     /locus_tag="VFV99_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060595.1"
                     /translation="MSDRKLAIAIAFATAAVLLTMVAVGMTTGATQEPHEHFAAPDAY
                     AKSLLADAGPLRLMFALDIAFCVLYTAFFIAFARHLRNLWAWLGLGAMIVVAVLDFVE
                     DHHIVSMLESAEHGILPGADSIMWQATESATKFSMSYLSLVLFGLAIPRTRTLGWVLA
                     IFLTVGTLVSAVVGYAVPPDVQPSIESGRWVGFLAGFALAVAWLWHEPDAT"
     gene            3339..3623
                     /locus_tag="VFV99_14610"
     CDS             3339..3623
                     /locus_tag="VFV99_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060596.1"
                     /translation="MGPLDVRDVSGRLWRWLWSVLAGAQIAVIVALVLPQRSLNGWGG
                     GAFGGANALLVAAGALGLALAINALLAALMRRPPRVRIRLPRARLVRGRC"
     gene            complement(3634..4197)
                     /locus_tag="VFV99_14615"
     CDS             complement(3634..4197)
                     /locus_tag="VFV99_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060597.1"
                     /translation="MRMVLVCLAVAGCGVKSNEPFLLDTADCSVVNNFKVDNQATPWT
                     DCRAYWSGTSNVLTVELTTPASMGSFLAPAPMWIRASVQVDADVNATLRSVAHPASAL
                     PSTVASDQIAIDLVVPGCGNIGANGVMIKTMADVGDKSVGFSLGIDGTCSEQTVMHAL
                     SGGFIVSAAAQAGSTAASDPATVVSVP"
     gene            4275..4550
                     /locus_tag="VFV99_14620"
     CDS             4275..4550
                     /locus_tag="VFV99_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060598.1"
                     /translation="MHDLDVRDLEGRFGRLVAAISIGAILAVPIAALLPQESMRGWGA
                     GAFGLANPVLVIAGACVIALGVNALLHATRSRTPPIRVPRATTRRRR"
     gene            complement(4547..6091)
                     /locus_tag="VFV99_14625"
     CDS             complement(4547..6091)
                     /locus_tag="VFV99_14625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5060599.1"
                     /translation="MTELDREPLKIKAVMGSSERRGAWKVPALVEAHVSFGNMELDLR
                     EAEMGPDTTIEAYVKMGNLEIIVPSDLAVDVNVDSIMGNVEQGARDGHGDGGEVQVSG
                     SAAAGQQLASHAYRDPTLPPQKRLRVTGKVKLGNCEIIPLRAGETREDYARRIRHIHR
                     MRHGHHMHGHHGRHQRHDRWREIQAERELWKTEPGKSPWLPWWLQAQMRRRIFSWFVI
                     TFAFGVLAGVKWVGSHPWWHFIFPAILLSITSGWVSWRLTRPLLMVVKAARDIGDGKL
                     DTRLHTSKRGEMRVLASAINDMASRIEQQIKDQRQLLAAVSHELRTPLGHMRVLVETA
                     RDTKDPKVLEEIDKEIVTLDDLVGRLLASSRLEFGNLDRRELDLGELIAEVANSAGIA
                     PDKIEAVGDVHAKVDPTLVRRAIANLLDNARVHGGGAIAVRAVRRGHQVAIEVDDAGP
                     GVQKDRRADAFRAFVPSSGGGLGLGLALVSRIAVAHEGGAWIDDRPGGGARVGFTVSV
                     APPAAA"
     gene            complement(6088..6456)
                     /locus_tag="VFV99_14630"
     CDS             complement(6088..6456)
                     /locus_tag="VFV99_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060600.1"
                     /translation="MTEDTNSLAVNVLLGSTERRGDWKVPARIDARVTLGHLQLDLRD
                     ADLGPDTTIDAVVTLGNLEILVPEDVVIDIDMDSIAANVDGGREPMAFGLPGVRRLRV
                     TGKVRFASCEVIPVPRGLFE"
     gene            complement(6449..7144)
                     /locus_tag="VFV99_14635"
     CDS             complement(6449..7144)
                     /locus_tag="VFV99_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5060601.1"
                     /translation="MRVLLIDDDARLAELLVGYLQPQGVTLVHAGGGQAGLGQLQGGS
                     FDAVLLDVMMPGMDGLAVLRKIRDAGHRVPVIMLTARGDEADRVVGLELGADDYVAKP
                     FSPRELLARLRAVLRRAAPEAVAEKLSAAGITVDTGAREAWVDGKSVELTGLEIDLLV
                     ALLRRAGRVVPRSALMELAGRGDVSVGERAVDVHISRLRKKLGDDAATRIRTVRGVGY
                     TLARTVDEEESHD"
     gene            complement(7199..7708)
                     /locus_tag="VFV99_14640"
     CDS             complement(7199..7708)
                     /locus_tag="VFV99_14640"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX5060602.1"
                     /translation="MICLALGLGVLGFVAARKARRCSRGYGWGWHHHHHHGWHGGWHG
                     GGPGRRGLYMALSHLDASPAQERAIVNEVDKLRERLHSAKRNLKDSRGDLAAAVRAPT
                     LDDAALGAVLGRVDTATGEMRSAALDALRNIHAVLDDKQREQLADILDRGWWRGRSHG
                     DHGGGPYRV"
     gene            7912..9672
                     /locus_tag="VFV99_14645"
     CDS             7912..9672
                     /locus_tag="VFV99_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5060603.1"
                     /translation="MVALIKSATLVGIDAVTIDVECVITSGQLPAFNIVGLPTTSVKE
                     GAVRIRSALHSVGHDVPQKKVTVNLAPADLRKPGSALDLPTALSIVIADGTFDGSLLD
                     SLLVLGELGLDGSVRAVPGVLAAAMLARKRRMRGILVPDRCAAEALVVDGIEVYGVRH
                     LSQVIAALAGEEELPQPSSASGRPRNRIFSADMSDVRGQDFARAAIEIAVAGGHNLLM
                     TGPPGTGKTMLARRIPSVLPTMTRDEALETTKIYSALGLADGGLITERPFRAPHHTIS
                     GAALHGGGTMPRPGEISLAHNGVLFLDELPEFARYTIEALRQPLEERAVTVSRINGMI
                     RLPSSFMLVAASNPCPCGWLQSGVRECTCSRFAVERYRARMSGPLLDRIDLQVYVRPV
                     ELKVLRDPKPSESSSRVRDRVMLARERQLARLSRFGIRCNAEMPPKVLRETCPLDDAC
                     ETYLKELVEVRQSLSARSIDRLIKVARTIADILGQDRIDRVCLREAADYRAIDPTADI
                     FAAPPPHASGRPLIARVRMNDETATVIVDSDDDGYEPWAPPPERESTDAEPPQVGHPE
                     PTPDDLAAATHSLPPLPPPP"
     gene            9744..10088
                     /locus_tag="VFV99_14650"
     CDS             9744..10088
                     /locus_tag="VFV99_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060604.1"
                     /translation="MTPLANRIYKQLVSHVRRKKASITYGELAAAVDVHHRSRKLHAA
                     LGEVTNACRHSQLPALPAIVWRADAKQPSDGYFKVAHPRAHTDEARVAAWEREHARVL
                     GAIEQFPTKLGE"
     gene            10133..10480
                     /locus_tag="VFV99_14655"
     CDS             10133..10480
                     /locus_tag="VFV99_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060605.1"
                     /translation="MRKTISLAQLAKNPEKIAKDIEASGSTYRIRRPGRKAMLLVGQD
                     YFDSWVASLEFIQRHPNWREELKQSELDYKAGLCRPLDEILEELGLDERPANTRRRGA
                     APRTATNRRASRR"
     gene            complement(10693..11346)
                     /locus_tag="VFV99_14660"
     CDS             complement(10693..11346)
                     /locus_tag="VFV99_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5060606.1"
                     /translation="MGATAFTRNHRDHSNDTGYQFEFFCDKCGNGYRSTFQASALGVG
                     SKIAKGLGALFGGNKLWGAGVAADHLKDGLRGAGWDEAFKKAIDEIKPKFHQCTRCGI
                     WVCPEVCWNDAKQLCENCAPDLAEEAASAQAHIAANQARQKMEAVDQTEGFDPKAQML
                     AGCPHCKARLAAGAKFCASCGKPVVQTQAQKKFCTGCGAQLAAGAKFCSGCGAASEG"
     gene            11374..12555
                     /locus_tag="VFV99_14665"
     CDS             11374..12555
                     /locus_tag="VFV99_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060607.1"
                     /translation="MVTSRWLLVSSVLCGVMGTAQAQPKQTLPAMVGANELMKVTTPS
                     GFVDDVVAYDNQRLAYVVADTSTKAELHVVQFGCAKCIEDKQEIVIDLAAVTLRPTSL
                     RLVGQRAFVIGATEDGNQIAALIDFAKKAPLYKLGPATHVTLITRDSKPVVAVHKASA
                     TKVGTKHDVELFALENGKRVAKGKAFELDKDTEKKLDFRVNHWSDGWTRAGGLKGGDW
                     NKKENQRSPDSEATYDLIAGKFVENKPIGDLFEQRKRYQILADAGGQLDFFRMKPDNT
                     AIEIWRKGALGAITLDQPLSQYDVKSLQGIIQPDGSAWIAIKVDPVNAEAVARKKADP
                     EYLDVFRVGTDGKATRKARILAKGVKHRFGVVDNYFWLLERSTGFDRGGRVLTVYQIS
                     A"
     gene            complement(12552..13460)
                     /locus_tag="VFV99_14670"
     CDS             complement(12552..13460)
                     /locus_tag="VFV99_14670"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX5060608.1"
                     /translation="MLDVVLSPEVAEARVDKRPIVALESTLVAHGMAWPDNFELAKDL
                     EKIVREAGAVPAHIAVVDGRPCVGLSSAQLERIAQQGSTFAKAGATDLAVHLARRKSA
                     ATTVSATSVIAARAGIRVFATGGIGGVHRGDAGDVSHDLIALAKTPIAVVSAGAKAIL
                     DLPRTMELMETLGVLVLGYRTDEMPAFYTVRSGIRLEHFTGTPGDCADILRRHWRELD
                     GGGVLVCNPIPAEASLDERLIASAIEDALRTARTTNITGKALTPHLLAQLADATAGAS
                     VRANKALARNNAQVAAEIALALGAYV"
     gene            complement(13479..13697)
                     /locus_tag="VFV99_14675"
     CDS             complement(13479..13697)
                     /locus_tag="VFV99_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060609.1"
                     /translation="MKFPLTQQLIDEAKRFELRSACCHCFFWNEKDQKCWHDWPDEGQ
                     RRWPLDAKDPETGAIPTEVAFCKEFELR"
     gene            complement(13694..13963)
                     /locus_tag="VFV99_14680"
     CDS             complement(13694..13963)
                     /locus_tag="VFV99_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060610.1"
                     /translation="MIRLLIWLALIGGFVFLGSTVKLGNRTFFGHVRAIWHTEEAQEL
                     KDGVKEKAGPAAGRIKRGIEAGYNAMKDDGGTGSAGSAAPAKHHP"
     gene            complement(14073..15941)
                     /locus_tag="VFV99_14685"
     CDS             complement(14073..15941)
                     /locus_tag="VFV99_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5060611.1"
                     /translation="MALVLALATSASAQTAGGDVPLDTFKPAMDSRGYLTVNASQVLG
                     NKEFSFGLGALDWGYKMLGFGDPDTCSAGTGAPCYQVNNMITATLIAAFGLKAGPAEL
                     EFGASVPFVIMSGDRGPNSLGADMMPGGNDDQEYKLDGQGIGNVGLHFKTRFLKTSRP
                     PHLGLGVIASVYLPTVSEKGKWLGDTKAVPQIMGILDKEFGRRGEFRVALNGGVRIRQ
                     NVTFTDSGVMGAPATNDSLTAGTEIPIGLGLAYAISVQKFDVVAELHGAIPIASGNDN
                     YFPLEAIGGVKLYLARNSFLSLGAGTGLLRKQETGANPDVRAFIGIVFEPNIGDRDGD
                     GIKDDVDKCPDDPEDYDRFEDEDGCPEPDNDRDGILDEDDKCPNIPEDKDGFQDEDGC
                     PEGNKNDRDGDGILDDVDKCPDDPEDFDQFQDEDGCPDPDNDQDGILDVDDLCPNDPE
                     DKDGFEDEDGCPDLDNDKDRILDKDDKCPNEPETYNGFEDEDGCPDRGRVVVTDTSIE
                     ILDMIYFEYDKAIIKKESYPILDAVAATLQGNPSISLIEIQGHTDERGNDAYNLDLSD
                     RRAKAVKDYLVGKGVDEKRLTSQGYGETQPLIRESNEKAWAKNRRVAFLILKRATD"
     gene            complement(16222..>17099)
                     /locus_tag="VFV99_14690"
     CDS             complement(16222..>17099)
                     /locus_tag="VFV99_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060612.1"
                     /translation="QVQTPENPDGSNQTVTPPANDLAGSIAVECKQLAAEKKWTDANS
                     CADRLQQHDGKLAKTLKAQYKQELENELTVGRISDAVKKNDLTRSRKELAQIEDGSVY
                     RGPAEKMVDDLEGDLVSRYHDEALALKRSNKCDQIEKLVGEARDKGGPKAAAAVLKEK
                     CSAPPPVEKDCSDKLVDNSKACKQQFCAKNGGDPRCGTGQVQVTPPANCDAEALKEEG
                     MQNINMGQHAAALSRFEMSLKCKRDPYVIQLAFMEACSSSNSPKAKYYYKQLTAAQQQ
                     KFAQICIRQKVDYLP"
BASE COUNT         2815 a         5707 c         5486 g         3091 t
ORIGIN      
        1 gaactcgagc atgccctgcg cctgccgctc gatgagccgc ttcttcagct ccttgaagta
       61 ctggcgatcg agccggcgcg tgccctcggc ggcggagcgc acgagcactt cgagatcggt
      121 caggccgatg ccggcggtga gcttgccgag ctcgggcacc tggtagtcgg agaacgacgc
      181 gacgtccttg ccggttgcct tcacctgcgc ggcgaggaac gcggtgcgct cggcctcgtt
      241 cggcagcggc acttcgatcg ccgcgacgtg cgggttgctg gacagccgct ccgcgacgct
      301 cgagagtcgc gggtcgatca gcacgaacgc catgttgagc cgcttgatat acgggctcga
      361 tgcccagttg agcagcgtca ccaggtgcgt ctgctcgttg agatcgaggt tgtcgccgct
      421 cggcgcgacg tagccgacgt gatcgatgat caccgcacac cgaatgcgct ggttgtcgtc
      481 ggccatcacg atcttctgga tcatgaggtc gagcgcgagc agacccttcg tcggatcgcg
      541 cggcagctgg cgcaggtcac cgatccactt gttcgcggtc gcaaccattt cctggagccg
      601 cttgctgctc gtgcctgccg cgcagcgtag gccgcgcgcg agatcgtagt ggacgacgag
      661 atcccagcga ccgaacagct gatcggcgat gaagtcggcg agcccggtcc agcgcggatc
      721 attctccgca ccggcgcgca cgacatcgaa cgtgttgccg tgcagcacga acatcgacgt
      781 cgtgcccgag aaatacagct cggcgagctt cgccgcccag cccgggaagt gcgcaggcag
      841 ctgtgccgcc ttcgtcactc catcggtcgc accggccagc gtcttcggat cgctggtctc
      901 cgccattacg acttctcgag gttctcgagc tctttgcggg ccgcttcagc ttcggccgga
      961 ccgaggtcct tctgtgcctg ttgcaccggc gtggtctccg gcgcgcgcat gccgaggtcg
     1021 acctcgaggc ccttgagcgc gtcttccgcc atctgcttct cgagccgctc gtcgtcctcg
     1081 atcttttcca tgccctgccg cgacatgtcg gcggcaacgc gggccttgcc gcggttcgcg
     1141 tcgatcttgc gctggatcgc atcttccatc tgaccgaagt cggtcgtgag atccatgttg
     1201 aacgtctcgg tcaacttcgc gatctcggcc tcggccgcgc tcatcttgag gtcggcgtcg
     1261 tacttctgga tcttgtcttt gacctcggcg agcttcttgt tcgcgaactg aatcttcttg
     1321 aggttgttgt tgtaggcctc ttcgtgcatc gcgagctgcg cctggttctc accgagctgc
     1381 gtcttcacct tggtcagctg cagcgcgatg atcgacgcct tgtcgcgatc gttcagcttc
     1441 aaataggcct tggcctgcgc ggtcagcttc gcgacttgcg cctcaccgtc cttgacctgg
     1501 cgcgtgacgc gctcgacgag accgcgatac tgctcgaggc cttcgcggcc ctctttgagc
     1561 tgttcgacag acttgtcgta ctcgtactgc atctgggcga tcggatcttt gttcgtgagc
     1621 caattgccca gcttgttgaa ctgcgccgcg atcgccttga agaacttggt cagaatcatc
     1681 gcaggtcctc cccgaggaac gtgaacctac caccacacga gcccgacacc cttgtatgct
     1741 gcgcagcaaa tgctgcgagt gggcctgttg gtggtcctgg ccttgcgcgc tggccgagcc
     1801 ggccgcactg acaaagctgg gttcgagaat gtggttgcag cgttcctcac tagtaatgtc
     1861 taaacgcggt ctggcaaggt cagcagaccc gaatccccag cagtaatggc cgataagaat
     1921 ccatccactg ctctcgcgct cgtcaatccg gtcggcctcg gtgtggccgc cggtgcggcg
     1981 acggtcgcga tcgccctgac gaacccgctc atcggcgtcg tcgggggcgg tgtgtggctg
     2041 gccacggtcg cgatcgatgc gatccggcgc agccgcaaga agaagaagcg cgcggccgtc
     2101 tcgatcccga tgatgaagga tccagacgac atcgtcgatc cgacgacccg cgcggcggtg
     2161 cagaagatcc tcgcgaacaa ggccgagcta cagaagatcc tcgacgagac gccggccgac
     2221 ctgcagatcc acatctcgaa gacactcggc tcgctcgacg agctcgagat gtacgcggtt
     2281 cgcctcgtcg aacgcgcaga agacatcacg acgcacctct cgaagatgaa cctgcccgcg
     2341 ctggtcgccg aggtgaagca gctcgcgaca cgcgccgcgg ccgcgaagga tccagtgacg
     2401 cgtcagtcgt acgaagaggc gaagcaggcg cggatggacg agatccgctc gctcaaagag
     2461 atcaagacga cgaaggatcg catcgacgca aatctaatgc gcgtggtcgc ggtgatgggc
     2521 gcgctgccga cgaaggtggt ccacatgcgc gcgctcgacg cgcaggcact cgatcagctg
     2581 tcgggcgaca tctcgcacga tctatcggcg atcggtgaag agctgaagac gagcgaggcc
     2641 gtgatcaagc agcaactggt cgcgcagtga gcgcgcccgt gagcgaccgc aagctcgcca
     2701 ttgcgatcgc gttcgccaca gccgccgtgc tgctcacgat ggtcgcggtc ggcatgacga
     2761 ccggcgcgac gcaggagccg cacgagcact tcgcggcacc cgatgcctac gcgaagtcgc
     2821 tgctcgccga cgcgggcccg ctgcgcctga tgttcgcgct cgatatcgcg ttctgcgtgc
     2881 tctacacggc gttcttcatc gcgttcgcgc gccatctgcg caacctgtgg gcgtggctcg
     2941 gcctcggcgc gatgatcgtc gtagccgtgc tcgacttcgt cgaggatcat cacatcgtgt
     3001 cgatgctcga gagcgcggag cacggcatcc tgccgggcgc ggattcgatc atgtggcagg
     3061 cgaccgagag cgcgacgaag ttctcgatgt cgtatctgtc gctcgtgctg ttcggcctcg
     3121 cgattccacg tacgcgcacg ctcggctggg tgctcgcgat cttcctcacc gtcggcacgc
     3181 tggtctcggc ggtcgtcggg tacgcggtgc cgccggacgt ccagccgtcg atcgagagtg
     3241 gccggtgggt cggtttcctc gccggattcg cgctggcggt cgcctggctg tggcacgaac
     3301 ctgacgcaac ctgacgtcgc ggctggtgtc tacgtggcat gggcccactc gacgtacgtg
     3361 acgtgtccgg ccggctgtgg cgctggctct ggtccgtgct cgcgggcgcg cagatcgcgg
     3421 tcatcgtcgc gctggtcttg ccccagcgct cgttgaacgg ctggggtggt ggtgcgttcg
     3481 gcggcgcaaa tgcgctgctt gtcgcggcgg gtgcgctcgg cctcgcgctc gcgatcaacg
     3541 cgctgcttgc cgcgctcatg cgtagaccac cgcgtgtccg catccggttg ccgcgcgcaa
     3601 ggctcgtgcg cggtcgctgt taggacgcag cactcacggt accgagacga cggtcgccgg
     3661 atcgctggcc gcggtcgagc ccgcctgcgc ggccgcggac acgatgaagc caccgctcaa
     3721 agcatgcatg acggtttgtt ccgagcaggt cccatcgatg ccgagcgaaa agccaacgct
     3781 cttgtcgccg acgtcggcca tcgtcttgat catcacgcca ttcgcgccga tgttgccgca
     3841 cccggggacg acgaggtcga tcgcgatctg atcgctcgcg accgtcgaag gcagcgcgct
     3901 cgcagggtgc gcgacgcttc gcaacgtggc gttgacgtcc gcgtccactt gcacgctggc
     3961 gcgaatccac atcggcgcgg gtgccaggaa cgagcccatc gaggccggcg tcgttagctc
     4021 gacggtaagg acgttgctcg tacccgacca gtacgcgcga cagtcggtcc acggcgtcgc
     4081 ttggttgtcg accttgaagt tgttcacgac actgcagtct gcggtgtcga ggagaaacgg
     4141 ctcgttgctc ttcacgccac agcctgcgac ggcgaggcac accaggacca ttcgcatgat
     4201 ttctcctaag acctgtcaag cagtctaccc gacgttcggt cgcaaccgga tggccaccgt
     4261 cgtgtctacg tggcgtgcat gacctcgatg tccgcgatct cgaaggccgc ttcggccgat
     4321 tggtcgcggc gatctcgatc ggcgcgatcc tcgcggttcc catcgccgcc ctgttgccgc
     4381 aggaatcgat gcgcggctgg ggcgctggcg cgttcgggct cgcaaatccg gtgctcgtga
     4441 ttgccggagc gtgcgtgatc gcactcggcg tcaatgcgct gctccacgcg acgcggtcgc
     4501 gtacgccgcc gattcgcgtg ccacgtgcga ccacgcgacg ccgccgctaa gcagcagccg
     4561 gcggcgcgac cgacacggtg aagccgacac gcgcgccgcc gccggggcga tcatcgatcc
     4621 acgcgccgcc ttcgtgcgcg accgcgatcc gcgacacgag ggcgagaccg aggccgagcc
     4681 caccgccgct cgacggcacg aatgcacgaa acgcgtcggc gcggcgatcc ttttgcacgc
     4741 cggggccagc gtcatcgacc tcgatcgcga cctgatggcc gcgacgaacc gcgcgcacgg
     4801 cgatcgcgcc gccaccatgg acgcgcgcgt tgtcgagtag attggcgatg gcgcgacgga
     4861 cgagcgtcgg atcgaccttc gcgtgcacgt cgccaaccgc ctcgatcttg tcgggcgcga
     4921 tgccggcgct gttcgcgacc tcggcgatca gctcgccgag atcgagctcg cggcgatcga
     4981 ggttgccgaa ctcgagccgc gagctcgcga gcaagcgccc gacgaggtcg tcgagcgtga
     5041 cgatctcttt gtcgatctct tcgaggacct tcgggtcctt ggtatcgcgc gcggtctcga
     5101 cgagcacgcg catgtggccg agcggcgtgc gcagctcgtg cgacaccgcc gcgagaagtt
     5161 ggcgctgatc tttgatctgc tgctcgatgc gcgatgccat gtcgttgatc gccgacgcga
     5221 gcacgcgcat ctcaccacgc ttcgacgtat ggagccgcgt atcgagcttg ccgtcgccaa
     5281 tatcgcgcgc cgccttgacg accatgagca gtggccgggt cagccgccac gacacccagc
     5341 ccgacgtgat ggacagcaga attgccggaa agatgaaatg ccaccacggg tgcgagccga
     5401 cccacttcac gccggcgagc acgccgaacg cgaacgtgat cacgaaccag ctgaagatgc
     5461 ggcgacgcat ctgcgcctgg agccaccacg gcagccacgg cgatttgccc ggctcggtct
     5521 tccacagctc gcgctccgcc tggatctcgc gccagcggtc atggcgctgg tgacgaccat
     5581 ggtggccgtg catgtggtgg ccatgccgca tgcgatggat gtgacgaatg cgacgcgcgt
     5641 agtcctcgcg cgtctcgccc gctcgcagcg gaatgatctc gcagttcccg agcttcacct
     5701 tgccggtgac gcgcaggcgc ttttgcggcg gcagcgtcgg atcgcgatac gcatggctcg
     5761 cgagctgctg acccgccgcc gcgctgccac tgacctggac ctctccgccg tcgccatggc
     5821 cgtcgcgcgc gccctgctcg acgttcccca tgatcgaatc gacattgacg tcgactgcga
     5881 gatcgctggg cacgatgatc tcgaggttac ccattttgac gtaggcctcg atcgtcgtgt
     5941 ccgggcccat ctccgcttcg cgcagatcga gctccatgtt gccaaagctg acgtgggcct
     6001 cgacgagcgc tggcactttc caggcgccac ggcgctccga cgaacccatc accgccttga
     6061 tcttcagcgg ctcgcggtcg agctcggtca ttcgaacagg cctcgcggga ctggaatcac
     6121 ttcgcagctc gcgaagcgca ccttgccggt cacgcgcagt cgccgcacgc cgggcagccc
     6181 gaacgccatc ggctcgcggc caccgtcgac gttcgccgcg atcgagtcca tatcgatgtc
     6241 gatcacgacg tcttcgggaa ccaagatctc gaggttgccg agcgtgacga ccgcgtcgat
     6301 cgtcgtgtcc gggccgagat cggcgtcgcg cagatcgagc tgcagatggc cgagcgtcac
     6361 gcgcgcgtcg atgcgcgcag gaactttcca atcgccgcgg cgttcggtcg aaccgagcaa
     6421 aacgttcacc gcgagcgaat tcgtgtcttc agtcatggga ttcttcctca tcgaccgtgc
     6481 gcgcgagcgt gtagccaacg ccacgcacag tccggatccg tgttgccgcg tcgtcgccaa
     6541 gcttcttgcg cagacgcgag atatgaacat cgacagcgcg ctcgccgacc gagacgtcgc
     6601 ctcggcccgc cagctccatc agcgcgctgc gtggaaccac gcgtcctgca cgacgcagta
     6661 gcgcgaccaa tagatcgatt tcgagccccg tgagctcgac ggacttgccg tcgacccacg
     6721 cttcgcgcgc accggtgtcg acggtgatgc cggctgcgct gagcttctcg gccaccgctt
     6781 ccggcgccgc acgcctgagc accgcacgca accgtgcgag caattcacgc ggcgagaacg
     6841 gctttgcgac gtaatcgtcg gcgccgagct cgagcccgac gacccgatcc gcttcatcgc
     6901 cgcgcgcggt caacatgatg acgggaacgc ggtggccggc gtcgcggatc tttcgaagaa
     6961 ccgcgagacc atccatgccg ggcatcatca cgtcgagcag caccgcatcg aagctgccgc
     7021 cttgcagctg accgagaccc gcctgcccgc cgccggcgtg caccagcgtc acgccctgcg
     7081 gctgcaggta gccgaccagc agctcggcca gtcgggcatc gtcgtcgatc agaagtacgc
     7141 gcacggtggg atcatctcat caggagggtg tcgagtctgc tttgcgtcgg cctaccattc
     7201 agacgcggta cggaccacca ccgtgatcgc catggctgcg gccacgccac cacccgcgat
     7261 ccaggatgtc ggcaagctgc tcgcgctgct tgtcgtcgag gacggcgtgg atgttgcgga
     7321 gcgcgtcgag cgccgcgcta cgcatctcac cggtggcggt atcgacacgg ccgagcaccg
     7381 cgccgagcgc ggcatcatcg agcgtgggtg cgcgcaccgc cgcagcgaga tcaccgcgac
     7441 tgtctttcag gttgcgcttc gcgctgtgca gccgctcgcg cagcttgtcc acctcgttga
     7501 cgatcgcgcg ctcctgcgcg ggcgacgcgt cgaggtgcga gagcgccatg tacaagccgc
     7561 gccggccggg cccgccgccg tgccagccgc catgccagcc gtgatgatga tgatggtgcc
     7621 aaccccatcc gtagccgcgg ctgcaacgac gggctttgcg agccgcgaca aaaccgagga
     7681 cgccaagtcc gagtgcgaga caaatcatgg gttcgttcct ttcaccagct caggatggag
     7741 agcgcgtgtt tcggaacctg cagccgcaat atgaagaaac cttgcagcgc gtaagtggcc
     7801 ggcgtccggc cggatttcgg ccacgccgtc cgcaccgcgg acccttgcgc acgcaactcg
     7861 cgagaatcgc gtagcccaat tctggcgcgc gccgtgctga tgcgcggcgg catggtcgcg
     7921 ctcatcaagt cagccacgct cgtcggcatc gatgcggtca cgatcgacgt cgagtgcgtg
     7981 atcacgtccg ggcagttgcc ggcgttcaac atcgtcggtc tgccgacgac ttccgtcaaa
     8041 gaaggtgcgg tgcgcatccg aagcgcgctg cactcggtag gtcacgacgt gccgcagaag
     8101 aaggtcacgg tcaatctcgc gcccgccgat ctgcgcaagc ccggcagcgc gctcgatcta
     8161 ccgacggcac tgtcgatcgt gatcgcagac ggcacgttcg atggctcgct actcgatagc
     8221 ttgctcgtgc tcggcgagct cggcctcgat ggctccgtcc gcgcagtgcc cggtgtgctc
     8281 gccgccgcga tgctcgcacg caagcgtcgc atgcgcggca tcctcgttcc ggatcgctgc
     8341 gcggccgaag cgctcgtcgt cgacggcatc gaggtctacg gcgttcgcca cctgtcgcag
     8401 gtcattgcgg cgctcgccgg tgaagaggag ctgccgcaac cgtcttcggc gagcgggcga
     8461 cctcgcaacc gcatattcag cgcggatatg tcggacgtgc gcggtcagga cttcgcgcgc
     8521 gccgcgatcg agatcgccgt tgcaggcggc cacaacctgc tgatgaccgg tccgcccggc
     8581 accggcaaga cgatgctcgc ccgtcgcatt ccgagcgtgt tgcccacgat gacacgcgac
     8641 gaagcactgg agaccacgaa gatctattcg gccctcggtc tcgcagacgg cggcttgatc
     8701 accgagcggc cgttccgtgc accgcaccac acgatcagcg gcgctgcgct gcatggcggc
     8761 ggcacgatgc caaggcccgg cgagatctcg ctcgcgcaca acggcgtgct gttcctcgac
     8821 gagcttcccg agttcgcgcg ctacacgatc gaagcgctgc gccagccgct cgaggagcgt
     8881 gcggtcacgg tcagccggat caacggcatg atccgattgc cctcatcgtt catgctggtc
     8941 gcggcatcga atccgtgccc gtgcggttgg ctgcagtcag gcgtccgcga gtgcacgtgc
     9001 tcgcggttcg ccgtcgaacg ctatcgcgcg cgcatgtcag gcccgctgct cgatcgcatc
     9061 gacctccagg tctatgtgcg cccggtcgag ctgaaagtcc tgcgcgatcc gaagccatcc
     9121 gagagctcgt cgcgcgtccg cgaccgcgtg atgctcgcgc gcgaacggca gctcgctcgg
     9181 ctctcgcggt tcgggatccg ctgcaacgcc gagatgccac cgaaggtgct gcgcgaaacc
     9241 tgcccgctcg atgacgcgtg cgagacgtac ctcaaagagc tcgtcgaggt acgccagtcg
     9301 ttgagcgcac gttcgatcga tcgtctgatc aaggtcgcgc gcacgattgc ggacatcctc
     9361 ggccaagacc gcatcgatcg tgtctgtctg cgtgaagcgg ctgattaccg cgcgatcgat
     9421 ccaacagccg acatcttcgc cgcgcctcca ccgcacgcga gtggccgtcc gctgatcgcg
     9481 cgcgtccgca tgaacgacga gacagcgacg gtcatcgtcg acagcgacga cgacggctac
     9541 gagccgtggg cgcctccgcc agagcgcgag tcgaccgacg cggaaccgcc gcaggttggg
     9601 catcccgaac cgacgcccga tgatcttgct gccgcgacgc actcgttgcc accgctgccg
     9661 ccgccgccct gaccgcgccg gccatccgtt tcgccgccgg catgtcgccg gcgatctgtc
     9721 gtgcacttcg aaaggaacct gtcatgaccc cgcttgccaa ccgaatctac aaacagctcg
     9781 tctcccacgt tcgccgcaag aaggcatcga tcacgtacgg cgagcttgcc gccgctgtcg
     9841 acgttcacca ccgcagccgc aagctgcacg ctgcacttgg cgaggtgacg aacgcgtgcc
     9901 gccactcgca gttgccggca ttgcccgcga tcgtgtggcg cgccgacgcg aagcagccga
     9961 gcgatggcta cttcaaggtc gcgcatccgc gtgcgcacac cgatgaggcg cgcgtcgcgg
    10021 cgtgggagcg cgagcacgcg cgcgtgctgg gcgcgatcga gcaatttccg acgaagctcg
    10081 gcgagtgagt tggacattgc cgtgctcgaa gccaacccat atgctggagg agatgcgcaa
    10141 gacgatctcg ctggcccagc tcgcgaagaa ccccgagaag atcgcgaagg acatcgaggc
    10201 gtccggatct acgtatcgca tcaggcgtcc cggccggaaa gcgatgctcc tggtggggca
    10261 ggactatttc gacagctggg ttgcatcgct ggagttcatc cagcgccatc caaactggcg
    10321 cgaagagctg aagcagtcgg agctcgatta caaggccggc ctctgccggc cgctagacga
    10381 gattctcgag gagctcggcc tcgatgaacg tccggctaac accaggcgcc gaggcgcagc
    10441 tccgcgcact gccaccaatc gccgcgcgag ccgtcgctga cgtattgcga cagctgaagg
    10501 ttacgccccg actcgggcga cagatgcaag atgccctaga cctcggagcg tttttcacga
    10561 agctcgtcat cgttcgacga cgacgacggt ggacgcttca catcgtctat cgggtccgtg
    10621 aaaccgaagt gctggtgatg cacatcgatc catcgtgggt gcgacgtaca actgtcgctg
    10681 atcgctaatc gctcagcctt cagaggcagc accgcagcca ctgcagaact tcgcgcccgc
    10741 cgcgagctga gccccgcagc cggtgcagaa cttcttttgc gcctgcgtct gcacgaccgg
    10801 cttgccgcaa ctcgcgcaga acttggcgcc ggcggcgagc cgcgctttgc agtgcgggca
    10861 gcccgcgagc atctgcgctt tcggatcgaa gccctcggtc tgatcgacgg cttccatttt
    10921 ttggcgggct tggttcgcgg cgatgtgcgc ttgagcgctg gcggcttctt cagcgagatc
    10981 gggggcgcag ttctcgcaga gctgcttggc gtcgttccag cagacctcgg ggcagaccca
    11041 gatgccgcaa cgggtgcact gatggaactt cggcttgatc tcgtcgatgg ccttcttgaa
    11101 ggcctcgtcc caccccgcgc cgcgcaggcc gtccttgagg tggtcagctg cgacgccggc
    11161 accccacagc ttgttgccgc cgaacagagc gcccagtccc ttcgcgatct tggaaccgac
    11221 accgagcgcg ctggcctgaa acgtcgagcg ataaccattc ccacacttgt cacagaagaa
    11281 ctcgaactga tagcccgtgt cgttgctgtg atcgcgatgg ttgcgagtga aagccgttgc
    11341 gcccatggaa ggccaggagc ataccatccg gccatggtga ccagccgctg gcttctcgtc
    11401 tcgtcggtgc tgtgtggggt aatgggtact gcgcaggcgc aacccaagca gacgttgccc
    11461 gcgatggtcg gtgcgaacga gctgatgaag gtcacgacac cgtcggggtt cgtcgatgac
    11521 gtcgtcgcgt acgacaacca gcggttggcg tatgtcgtcg cggatacgtc gacgaaggcg
    11581 gagctgcacg tcgtgcagtt cggctgtgca aagtgcatcg aagacaagca ggagatcgtt
    11641 atcgacctgg cggcggtgac gctgcggccg acgtcgctgc gtctggtcgg tcagcgcgca
    11701 ttcgtgatcg gcgcgaccga ggatggcaat cagatcgcag cactcatcga cttcgcgaag
    11761 aaggcgccgc tctacaagct cggcccggcg acgcacgtca cgttgatcac gcgcgacagc
    11821 aagccagtcg ttgcggtcca caaggcaagc gccacgaagg tcggaacgaa gcacgacgtc
    11881 gagctgttcg cgctcgagaa cggcaagcgg gttgcgaagg gcaaagcgtt cgagctcgac
    11941 aaggacaccg agaagaagct cgacttccgc gtcaatcact ggagcgatgg ctggacgcgc
    12001 gcgggcggcc tgaagggcgg cgactggaac aagaaggaga accagcgctc gcccgacagc
    12061 gaggcgacgt atgacctgat cgcgggcaag ttcgtcgaga acaagccgat cggcgatctg
    12121 ttcgagcagc gcaagcgcta tcagatcttg gccgatgcgg gtggtcagct cgacttcttc
    12181 cgcatgaagc cggacaacac ggcgatcgag atctggcgca agggcgcgct cggcgcgatc
    12241 acgctcgacc aaccgttgtc gcaatacgac gtgaagtcgc tgcaggggat catccagccc
    12301 gacggttcgg cgtggatcgc gatcaaggtc gatccggtga acgcggaggc ggtcgcgcgc
    12361 aagaaggcgg accccgaata cctcgacgtg tttcgcgtcg gcaccgacgg caaagcgacg
    12421 cgcaaggcgc gcattctcgc gaagggcgtc aagcaccgct tcggcgtcgt cgacaattac
    12481 ttctggctgc tcgaacggtc gaccggcttc gaccgcggtg gccgcgtgct gaccgtctac
    12541 cagatcagcg cttagacgta cgcgccgagc gcgagcgcga tctcggccgc aacctgtgcg
    12601 ttgttgcgtg cgagcgcctt gttggcgcga accgatgcgc ccgccgtagc atcggcgagc
    12661 tgcgcgagca ggtgcggcgt cagcgccttg ccggtgatgt tcgtggtgcg cgcggtgcgc
    12721 aatgcatctt cgatcgcgct cgcgatcaac cgctcgtcga gcgacgcctc agccggaatt
    12781 ggattgcaga cgagcacacc accgccgtcc agctcgcgcc agtgacggcg caggatgtcg
    12841 gcgcagtctc ccggcgtgcc cgtgaagtgc tcgagccgaa tgccggagcg aacggtgtag
    12901 aacgccggca tctcgtcggt gcgatagccg agcacgagca caccgagcgt ctccatcagc
    12961 tccatcgtgc gcggcagatc gagaatggct ttcgcgccgg ccgacacgac cgcgatcggc
    13021 gtcttcgcga gcgcgatcag atcgtgcgag acatcgcccg cgtcaccgcg atgcacaccg
    13081 ccgatgccgc cggtcgcgaa cacgcgaatg ccggcgcgcg ccgcgatcac gctggtcgcg
    13141 ctgaccgtgg ttgccgcgct cttccggcga gcgaggtgaa cggcgagatc ggtcgcgccc
    13201 gccttcgcga acgtgctgcc ttgctgcgcg atgcgctcga gctgtgcgct cgacagaccg
    13261 acgcacggcc gaccgtcgac gacggcgata tgcgccggca ccgcgcccgc ctcgcgcacg
    13321 atcttctcga ggtctttcgc gagctcgaag ttatccggcc acgccatgcc atgcgcgact
    13381 agcgtcgact cgagagcgac gatcggtcgc ttgtcgacac gcgcttcggc aacctcgggt
    13441 gaaagcacca cgtccagcac gcgccgattg taaatcgatc agcgcagctc gaactcttta
    13501 caaaacgcga cttcggtcgg aattgcgcca gtctcgggat ccttcgcatc caaaggccag
    13561 cgccgttggc cttcatcggg ccaatcgtgc caacacttct gatctttttc gttccagaag
    13621 aagcagtggc agcacgcaga gcgcagctcg aaccgcttcg cctcgtcgat cagctgctgg
    13681 gtcagcggga acttcacggg tgatgtttgg ccggcgccgc cgaccctgcc gagcccgtgc
    13741 cgccgtcgtc cttcatcgcg ttgtagccag cttcgatacc gcgcttgatg cggcccgctg
    13801 ccggccccgc cttctccttc acgccgtcct tcagctcctg cgcttcttcg gtgtgccaga
    13861 tcgcgcgcac gtgaccgaag aaggtgcgat tgccgagctt gaccgtcgag cccaggaaca
    13921 cgaacccgcc gatgagagcg agccagatga gcaatcggat cacgcccgga gtctagcccg
    13981 aaaaatctgc cgcgccccct tttcgctcgg cggccgaaac cacgaagcgc cgcccgtcac
    14041 acgagcggcg cttttgaagc aatgctcgcg agttagtcgg tcgcgcgctt caagatgagg
    14101 aacgcgacgc ggcggttctt cgcccacgcc ttctcgttcg actcgcgaat cagcggctgc
    14161 gtctcgccgt agccctgcga ggtcagtcgc ttctcgtcga cgcccttgcc gaccaggtag
    14221 tccttcacgg ccttcgcgcg gcgatcggag aggtcgaggt tgtacgcgtc gttgccgcgc
    14281 tcgtcggtgt gaccttggat ctcgatgagc gagatgctcg ggttgccctg cagcgtcgcc
    14341 gcgacggcgt cgaggatcgg gtacgactcc ttcttgatga tcgccttgtc gtactcgaag
    14401 tagatcatgt cgaggatttc gatcgacgtg tcggtgacga cgacgcgacc acgatcgggg
    14461 cagccgtcct catcctcgaa gccgttgtat gtctccggct cgttcgggca cttgtcgtcc
    14521 ttgtcgagga tgcggtcctt gtcgttgtcg agatcggggc agccgtcttc gtcttcgaag
    14581 ccgtccttgt cttccggatc gttcgggcac agatcgtcga catcgaggat gccgtcctga
    14641 tcgttgtccg gatcggggca gccgtcttcg tcctggaact gatcgaagtc ctcgggatcg
    14701 tcggggcact tgtccacgtc gtcgaggatg ccgtcgccgt cgcgatcgtt cttgttgcct
    14761 tcggggcagc cgtcttcatc ctggaagccg tccttgtcct ccgggatgtt ggggcacttg
    14821 tcgtcctcgt cgaggatgcc gtcgcggtcg ttgtcgggct cgggacagcc gtcctcgtcc
    14881 tcgaaccgat cgtaatcctc gggatcgtcg ggacacttgt ccacgtcgtc cttgatgcca
    14941 tcgccgtcgc ggtcaccgat gttcggctcg aacacgatgc cgatgaacgc gcgcacgtcc
    15001 gggttcgcgc ccgtctcttg cttgcggagc aggccggtgc cagcgccgag cgagaggaac
    15061 gagttgcgcg cgaggtagag cttcacgccg ccgatggcct cgagcgggaa gtagttgtcg
    15121 ttgccggacg cgatcggaat cgcgccgtgc agctcggcaa caacgtcgaa cttctgcacc
    15181 gagatcgcgt acgcgagccc caggccgatc ggaatctcgg tgcccgcagt cagcgagtcg
    15241 ttcgtcgcgg gcgcgcccat cacaccgcta tcggtgaacg tcacgttctg gcgaatacga
    15301 acgccgccgt tcagcgcgac gcggaactcg ccgcgacgac cgaactcctt gtcgaggatg
    15361 cccatgatct gcggcaccgc cttggtgtcg ccgagccact tgcccttctc ggagaccgtc
    15421 ggcaggtaga cgcttgcgat gacgccgagg ccgagatgcg gcggccggct cgtcttgaga
    15481 aatcgcgtct tgaagtgaag gccgacgttg ccgatgccct gaccatcgag cttgtactcc
    15541 tggtcgtcgt tgccgcccgg catcatgtcg gcaccgaggc tgttcggtcc gcgatcgccg
    15601 gacatgatca cgaacggaac agaggcgccg aactcgagct cggctggtcc cgccttcaaa
    15661 ccgaacgctg cgatcagcgt cgcggtgatc atgttgttga cctggtagca gggcgcgccc
    15721 gtgcccgcgc tgcacgtgtc cgggtcgccg aagcccagca tcttgtagcc ccagtcgagg
    15781 gcaccgaggc cgaacgagaa ctccttgttg ccgagcacct gcgatgcgtt gaccgtcagg
    15841 tagccgcgcg agtccatcgc cggtttgaag gtgtcgagcg gaacgtcacc gcccgccgtc
    15901 tgcgccgacg ctgacgttgc cagcgcgagc acgagtgcca ccgaaccaag agatcgccga
    15961 aaagtagaaa gcacaaggcc tccagacatg tagcctccga gctagtgcac ccaggctctg
    16021 ccctggttac accaacccga tcccccgggt caaacaagcg gcataacaga gttttccacc
    16081 cgatcacatt cccgttcgaa cgcgatgcgg tccggccggt ccgcggggac gacgaccggc
    16141 accccggaca tccagttcac ggtgacccgc atcgacgacc ggcacccgga cacccagttc
    16201 gaccgcgcgc cagtcggcgc tctacggcag gtagtcgacc ttttggcgga tgcagatctg
    16261 ggcgaacttt tgctgctgcg ccgccgtgag ctgcttgtag tagtacttcg ccttcggcga
    16321 gttgctgctc gagcacgctt ccatgaacgc gagctggatg acgtacggat cgcgtttgca
    16381 cttgagcgac atctcgaacc gcgacagcgc ggccgcgtgc tggcccatgt tgatgttctg
    16441 catgccctct tccttgaggg cctcggcgtc gcagttcgcc ggcggcgtca cctggacctg
    16501 accggtgccg cagcgcgggt cgccgccgtt cttggcgcag aactgctgct tgcacgcctt
    16561 cgagttgtcg acgagcttgt ccgagcagtc cttctcgacc ggtggcggcg ccgaacactt
    16621 ctctttgagc acggccgcag cggccttcgg gccacccttg tcgcgcgctt cgccgacgag
    16681 cttctcgatc tgatcgcact tgttgctgcg cttcagcgcg agcgcttcgt cgtggtagcg
    16741 cgacaccagg tcgccctcga ggtcgtcgac catcttctcg gccgggccgc gatacacgga
    16801 gccgtcctcg atctgcgcca gctccttgcg ggaacgggtc aggtcgttct tcttcaccgc
    16861 gtcggagatg cggccgaccg tcagctcgtt ctcgagctcc tgcttgtatt gcgccttgag
    16921 cgtcttcgcg agcttgccgt cgtgctgctg caggcggtcc gcgcagctgt tggcgtcggt
    16981 ccatttcttc tcggccgcga gctgcttgca ctcgaccgcg atcgagcccg ccaggtcgtt
    17041 cgccggcggc gtcacggtct ggttcgagcc gtcgggattc tcgggcgtct gcacctggg
//
LOCUS       DASUFX010000367         5495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1726945, whole genome shotgun sequence.
ACCESSION   DASUFX010000367 DASUFX010000000
VERSION     DASUFX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726945"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFV99_14695"
     CDS             complement(<1..1292)
                     /locus_tag="VFV99_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060613.1"
                     /translation="MVDLNRVDWQRSNKHHPIFMRFQVLAGVVAALASVVLLTEPVCA
                     EGHGKATARGPSSKSDGTDFTTEARDMFRVAACGSDEAIAARFAKTAKQTIDNHCERM
                     GRIYSWYRVSWMDKAKPFIAKLRPADAPTTVVYPFGGGDLSSALTVFPDATELTTISL
                     EAAGDIRTIDTIGTDRLKDDLSTTAKSISRLYNAAHSTTQNLMVASHAELPGTIMFAL
                     AGLAVHDMVPLQLRYFELEADGSIRYLTTDEMDAREAEIVASRKAKHSKHFWYEQTSA
                     FANVEITFRPRGDEKATPRVYRHILANLDNAHLTADKRLLEHLRAKGNVSVMTKAASF
                     LLWWDDFSQIRDHLLKTTAWMISDASGIPPRYADKAGFEQTTFGDFTGPYFIQDPINA
                     RAEFVKLWADQPHRDLPFRFGYPDKEKHNHLLVTRPKTR"
     gene            1363..2031
                     /locus_tag="VFV99_14700"
     CDS             1363..2031
                     /locus_tag="VFV99_14700"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEX5060614.1"
                     /translation="MKRIALIAVALAFAATGCHEPHSVLVKRPENKDVDSAVTAMWSH
                     EIHGVAQDGDWILSRSYFLLADGIAKTTGGVDLSHASIYDAHRDTVIEAVGSGVREIP
                     LSELVQRNHYLIVVRPSGMTAAQRARSVERARSRIGTEFDKAGLFGFDNPDKLYCSEL
                     VWWAAQTELRTGDHQRVITPSELMDYGQVIYWSGQRTDQQVMDLAMARDAQPDYGNPQ
                     YAAN"
     gene            complement(2098..3450)
                     /gene="aroA"
                     /locus_tag="VFV99_14705"
     CDS             complement(2098..3450)
                     /gene="aroA"
                     /locus_tag="VFV99_14705"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5060615.1"
                     /translation="MKLIVEPATGPLNGTITVPGDKSIGHRALLFSLLSQTPIRVLGL
                     GDGADNGRSARAITALGAKIAKDGNAIVISGTGLDGMTAPTAPVDCGNSGTTIRLLCG
                     LLAGQRFPTTLIGDESLSKRPMRRVIEPLAQMGASIKGQGGTAQGGGLDVTPPLVVGP
                     ATGPLRALDYKLPMASAQVKTAILLAGLSADGATIVTEPGPSRDHSERMLAYLGAPLT
                     VSGRATTIDTRGWNRRLAGSGFEVPSDPSSSAFLVAAALVAGAREVRLPGICVNPTRT
                     GFLDAIKAMGGHVALEDERQAGPEPVATLVVRGAAAELKATEIAGDLAVRSIDELPIL
                     AVLAARARGTTVVRNAEELRVKESDRIATTVAMLRGFGIQCEAHPDGFTVEGRPDRPL
                     EPARVHADGDHRIAMAAAVAGLVATGTTIIDDADNVATSYPGFAAALTTLGATIRSDS
                     "
     gene            complement(3447..4355)
                     /locus_tag="VFV99_14710"
     CDS             complement(3447..4355)
                     /locus_tag="VFV99_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060616.1"
                     /translation="MRLLVVALVVCACGGRARPGAGTGPAIAAPTTLAEKMIALLPDG
                     AQLLVEIDLARLKANPAVGPVATQALARLGADAKLPGLPIAVQGSPLANADLIVLAAY
                     GVGTDHAASLTLLATKAEVAGAVRLSPELVVLGPEEWTAQVETRAGIAEHTPIAPSLS
                     LMPLRDHAMPEKAPGAVLRVTGQLPFDARVALARQTGLEVAPAQLSIWADVVDDVAVI
                     IDADAADPGDKKAKDAAARLQTSLTAVLGAVANAPALQGLGLTSAIRDARTITQRTWV
                     RTIITVGPRQLTRAVERARAMLGSAS"
     gene            complement(4352..5467)
                     /gene="hisC"
                     /locus_tag="VFV99_14715"
     CDS             complement(4352..5467)
                     /gene="hisC"
                     /locus_tag="VFV99_14715"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5060617.1"
                     /translation="MSTAWIDDFAPAHVRNLDVYQPGKPIEELERELGISGAIKVASN
                     ENPLGPSPKAMAAIPAALSSLHLYPDAGGFALRRALAKRYGVPIEQIALGNGSNDMLY
                     QLVLATCEPTDEVLSHRYAFLSYRLSAQVAGRPFIVADTTRDLSCDVDKLIAAITPRT
                     KLVVLGSPNNPTGSVVTRADAERVLAALPDRALLVMDEAYAEYAAQWPEIDHVDGLTL
                     QKQDKRVIVLRTFSKIYGLAGLRIGFGVADQAVIDILNRVGRTFHVSSLAMVGAVAAL
                     DDHDHVMISAKHARAAIEKMRGAVHGPSVRVYPSLANFVLIDCGKLSTPIYEKLLRMG
                     VIVRPMAAWGLPHHIRVSVAAHQDLPRVIEALNQALA"
BASE COUNT          877 a         1834 c         1866 g          918 t
ORIGIN      
        1 cgggtcttcg gcctcgtgac gagcaaatgg ttgtgcttct ctttgtcggg atagccgaag
       61 cggaacggca gatcgcggtg cggttggtcc gcccacagct tcacgaactc ggcgcgcgcg
      121 ttgatcgggt cctggatgaa atacggcccg gtgaagtcgc cgaacgtggt ctgctcgaag
      181 ccggccttat cggcgtagcg cggcgggatg cccgacgcgt cggagatcat ccatgcggtg
      241 gtcttgagca ggtgatcgcg gatctgcgag aagtcgtccc accagagcaa gaagctcgcg
      301 gccttcgtca tcacgctgac attgcccttc gcgcgcaggt gctcgagcaa tcgcttgtcg
      361 gcggtcaggt gcgcgttgtc gaggttcgcg aggatgtggc gatagacgcg tggggtggct
      421 ttctcgtcac cgcgcggccg gaacgtgatc tcgacgttcg cgaacgcgct cgtctgctcg
      481 taccagaagt gcttcgagtg cttcgctttg cggctcgcca cgatctctgc ttcgcgcgcg
      541 tccatctcgt cggtggtgag gtagcgaatg ctgccgtcgg cctcgagctc gaaatagcgg
      601 agctggagcg gaaccatgtc gtgcactgcg aggccggcga gcgcgaacat gatcgtgccc
      661 ggcagctcgg cgtgcgatgc gaccatgagg ttctgcgtcg tcgaatgcgc agcgttgtag
      721 agccggctga tgctcttcgc ggtcgtcgac agatcgtcct tgaggcgatc cgtgccgatc
      781 gtgtcgatcg tgcggatgtc gcccgcggcc tcgagcgaga tcgtggtcag ctcggtcgcg
      841 tcgggaaaca cggtgagcgc gctcgacaga tcgccgccgc cgaacggata gacgacggtc
      901 gtcggcgcgt cggcgggacg cagcttcgcg atgaacggct ttgccttgtc catccatgac
      961 acgcggtacc agctgtagat gcggcccatg cgctcgcagt ggttgtcgat cgtctgcttc
     1021 gcggtcttcg cgaaccgcgc ggcgatcgcc tcgtcgctgc cacacgcggc gacgcgaaac
     1081 atgtccctcg cttcggtcgt gaagtcggtg ccgtcgctct tcgacgacgg ccctcgcgcg
     1141 gttgccttgc cgtgcccctc ggcacacacg ggctctgtca gcagcacgac gctcgctaac
     1201 gccgcgacca ctcccgcaag cacctgaaac cgcatgaaga tcggatggtg cttgttcgac
     1261 ctctgccagt caacgcggtt gaggtccacc accgacagcc cctaagtggc cgggatcgct
     1321 cgatgaccct gtgcggcacg gagcgtggat ttagtccggg gcatgaaacg catcgctttg
     1381 atcgccgtcg ccttggcttt cgctgctacc ggctgccacg agccacacag cgtgctcgtg
     1441 aagcgtccgg agaacaagga cgtcgactcg gctgtcacgg cgatgtggtc gcacgagatc
     1501 cacggcgtcg cacaggacgg cgattggatc ctgtcgcgca gctacttctt gctcgcggac
     1561 ggcatcgcga agacgaccgg cggtgtcgac ctctcgcacg cgtcgatcta tgacgcgcat
     1621 cgcgacaccg tgatcgaagc ggtcggctct ggcgtgcgcg agattccgct ctcggagctc
     1681 gtgcagcgta accactactt gatcgtcgtg cgtccgagcg gcatgacggc ggcgcaacgt
     1741 gcgcggtcgg tcgagcgcgc gcgcagccgc atcggtaccg agttcgacaa ggcgggtctg
     1801 ttcggcttcg acaacccgga caagctctac tgctcggagc tcgtgtggtg ggcggcgcag
     1861 accgagctgc gcaccggcga ccatcagcgc gtgatcacgc cgtcggagct catggactac
     1921 ggccaggtca tctactggag cggccagcgc accgatcagc aggtgatgga cctcgcgatg
     1981 gcgcgcgacg cgcagcccga ctacggcaac ccgcagtacg ccgcgaacta acgctttcgt
     2041 tcgagagaat ggcgcccgca gcttcgaaag ctgcgggcgc ttttgtttgg ctgtctgtta
     2101 actgtcgctg cgaatcgtcg cgccgagtgt cgtcagcgcg gccgcgaagc cgggatacga
     2161 ggtcgcgacg ttgtcggcat cgtcgatgat cgtcgtaccg gtcgcgacga gccccgcgac
     2221 cgcggccgcc atcgcgatcc ggtgatcgcc gtcggcatga acgcgcgccg gctcgagcgg
     2281 acgatcggga cgaccttcga cggtgaaccc atcgggatgc gcctcgcact ggatgccgaa
     2341 gccacgcagc atcgcgaccg tcgtggcgat gcgatcggat tccttcacgc gcagctcttc
     2401 ggcgttgcgt acgacggtcg tgccgcgcgc acgcgccgcg agcacggcga ggatcggtag
     2461 ctcgtcgatc gagcggaccg cgaggtcgcc agcgatctcg gtcgccttca gctcggccgc
     2521 cgcgccgcgc acgacgagcg tcgcgaccgg ctcggggccc gcctggcgct cgtcctcgag
     2581 cgcgacgtga ccacccatcg ccttgatcgc gtcgagaaat ccggtgcgcg tcgggttgac
     2641 gcagatgccc ggcaatcgta cttcgcgcgc gccggcgacg agcgccgccg cgacgaggaa
     2701 cgccgaggac gatggatcgc tcggcacctc gaacccggag ccggccagcc ggcgattcca
     2761 gccacgcgtg tcgatggtcg tcgcgcgacc gctgacggtc agcggcgcac cgagataggc
     2821 gagcatgcgc tcggaatgat cgcggctcgg gcccggctcg gtgacgatgg tcgcgccgtc
     2881 ggcggacagg ccggcaagca ggatcgccgt cttcacctgt gccgatgcca tcggcagctt
     2941 gtagtcgagc gcgcgcaagg gaccggtcgc cggcccgacg acgagcggag gcgtgacgtc
     3001 gaggccaccg ccttgcgcgg tgccaccctg ccctttgatc gacgcgccca tctgcgcgag
     3061 cggctcgatc acgcggcgca tcggtcgctt cgacagcgac tcgtcgccga tcagcgtcgt
     3121 cgggaatcgc tggcccgcga gcaggccgca caagaggcga atcgtcgtgc ccgagttgcc
     3181 gcagtcgacc ggcgcggtcg gcgcggtcat tccgtccagc ccggtgccgc tgatcacgat
     3241 cgcgttgccg tctttcgcga tcttcgcgcc gagcgcggtg atcgcacgcg ccgagcggcc
     3301 attgtcggcg ccgtcgccga gtccgagcac gcgaatcggc gtctgcgaca gcagcgagaa
     3361 caacagcgcg cgatgaccga tcgacttgtc gccggggacg gtgatggtgc cgttcagagg
     3421 tccggtggcg ggctcgacga tcagcttcat gacgcggacc cgagcatcgc acgagcgcgc
     3481 tcgaccgcgc gcgtgagttg gcgcggaccg acggtgatga tcgttcgcac ccaggtgcgc
     3541 tgcgtgatcg tacgagcgtc gcgaatcgcg ctcgtaagcc cgagcccctg cagcgctggc
     3601 gcgttagcca ctgccccgag taccgcggtc agcgatgtct ggaggcgggc agccgcatct
     3661 ttcgccttct tgtcgccagg gtccgcggcg tcggcatcga tgatcaccgc gacatcgtcg
     3721 acgacgtcgg cccagatcga cagctgcgcc ggtgccacct cgagaccggt ctgtcgcgcg
     3781 agcgcgaccc gcgcatcgaa gggcagctgc ccggtgaccc gcaggaccgc gcccggcgcc
     3841 ttctccggca tcgcgtgatc gcgtagcggc atcagtgaca gcgacggcgc gatcggcgtg
     3901 tgctccgcga tgcccgcgcg ggtttcgacc tgtgcggtcc actcctcggg accgagcacg
     3961 acgagctccg gcgacaaccg caccgcaccg gcgacctccg ctttcgtcgc gagcagcgtc
     4021 aggctcgccg cgtgatcggt tccgacgccg tacgccgcga gcacgatgag gtcggcgttc
     4081 gcgaggggtg agccttgcac cgcgatcggc agacccggca gcttcgcgtc ggcgccgagc
     4141 cgagcgagcg cttgcgtggc gaccggaccg accgccgggt tcgccttgag ccgcgcgaga
     4201 tcgatctcga cgaggagctg cgcgccgtca ggcaacagcg cgatcatctt ctcggcgagc
     4261 gtcgtcggtg cagcgatcgc cggcccggtc cccgcacccg gtcgtgcgcg gcctccgcac
     4321 gcacacacga cgagcgcgac gacgagcagc ctcatgcgag cgcttgattg agcgcttcga
     4381 tcacgcgcgg cagatcttgg tgcgcggcaa ccgacacgcg aatgtgatgc ggcaggcccc
     4441 atgccgccat cggccgcacg atcacgccca tgcgcagcag cttctcgtaa atcggtgtcg
     4501 acagtttgcc gcaatcgatg aggacgaagt tcgcgagcga cggatacacc cgaacgctcg
     4561 ggccatgaac cgcgccacgc atcttctcga tcgcagcgcg cgcatgcttc gcgctgatca
     4621 tcacgtgatc gtggtcgtcg agcgccgcga ccgcaccgac catcgccagc gacgacacgt
     4681 ggaacgtgcg accgacgcgg ttcaagatgt cgatcaccgc ctgatcggcg acgccaaagc
     4741 cgatgcgcaa ccctgccagc ccgtagatct tcgagaacgt gcgcagcacg atcacgcgct
     4801 tgtcttgctt ctgcagcgtg aggccatcga cgtgatcgat ctcgggccac tgcgccgcgt
     4861 actccgcgta cgcctcgtcc atcacgagta gcgcgcggtc gggcaatgca gcgagcacgc
     4921 gctcggcatc agcacgcgtg acgaccgagc cggtcggatt gttcggcgag ccgagcacga
     4981 cgagcttcgt gcgcggcgtg atcgcagcga tcagcttgtc gacatcgcac gatagatcgc
     5041 gcgtcgtatc ggcgacgatg aacggccgcc cggcgacctg cgccgacagc cggtacgaca
     5101 agaacgcgta gcgatgggac agcacctcgt cggtcggctc gcatgtcgcg agcacgagct
     5161 ggtacagcat gtcgttgctg ccattgccga gcgcgatctg ttcgatcggc acgccgtagc
     5221 gtttcgccag cgcgcggcgc agcgcgaatc cgccggcatc cgggtagagg tgcaggctcg
     5281 acagcgcggc cgggatcgca gccatcgctt tcggtgaagg ccctagtggg ttctcgttcg
     5341 aggcgacctt gatcgcgccg gagatgccga gctcgcgctc gagctcctcg atcggcttgc
     5401 ccggttggta gacgtctaag ttgcgaacat gagccggggc aaaatcatcg atccaggcgg
     5461 tggacacagc ggtggcaccc taccgcccgg aacag
//
LOCUS       DASUFX010000368         5321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_58391,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000368 DASUFX010000000
VERSION     DASUFX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58391"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFV99_14720"
     CDS             <1..576
                     /locus_tag="VFV99_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060618.1"
                     /translation="RKKLETIAKHAKFRVTLNSVIGSAPAGEPAEVVAFAKEHGFKPR
                     VSLVHDGNGQFKLEPEQRAEFDHLKHLSGGGRMEAAGDYRGKLMAEGKAPFKCRSGSR
                     YLYVDEFGIVRWCSQTRDRWGVPLSDYSRAELKRQFDTKKECNERCTVGCVRKASGPD
                     SWRGQSQPDPGVPAEQLVQIGNKPPSASVQP"
     gene            complement(579..1838)
                     /locus_tag="VFV99_14725"
     CDS             complement(579..1838)
                     /locus_tag="VFV99_14725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5060619.1"
                     /translation="MFDAVVLATAPTANDKLLGITLAERGRRVADKAGARRVYMIDSE
                     AALAGLAAWDRDRGDAALLFVRAGDQLVHLPLVRPLIAGLDPRRIAVGPTGEYAGALW
                     ADAAHGREIIAALTLSVESAEQNLVAGWQSAERIRHDDIAVHRATTPQEKRGAERMLL
                     QLLVKASEDSPVSKYVYRPLSRPLTRLLLHTSITPNQVSYFVGVLGLAGCVLTAFPSY
                     AALVWGAVLVFWSCVIDGCDGEIARMKLQFSPFGAWLDTVIDELTQVSYFFAIGYHTY
                     QHHPSWWLGGSIALGGISYAATIYAIYYFSLVVIKKGGSQYYEGDLEIVDDGGMPALR
                     KRVKVSTAPAWAKALGQIFLYMIRRDFINLAAMIVAFFDGYVVIYAGIWLGTIVAGAI
                     IVPEHVRLRRQLAEIKRRGGVPRYVAA"
     gene            complement(1843..2568)
                     /locus_tag="VFV99_14730"
     CDS             complement(1843..2568)
                     /locus_tag="VFV99_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX5060620.1"
                     /translation="MKAVILAAGVGSRLAPLTNDRPKVLVPVLGRSLLFRQLDWLAAA
                     GVASEDVVVVGGYRIDQLKASLDEGGFRCKVVLNEKYEPWGNFYSVLVAEPYLRGHDF
                     LQLDGDVILDDKILPRLIAAPGDALLATDTSAELDDDAMKVEMRGNQVYALDKKLIAA
                     NCIGEYIGVTKLSATAGAAVFRELAKFPAENLTHEYYDHSYHRLTQRGEVPFGIVKVD
                     DCRVLEIDDHADLKRAEAVLAQG"
     gene            2721..4583
                     /locus_tag="VFV99_14735"
     CDS             2721..4583
                     /locus_tag="VFV99_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5060621.1"
                     /translation="MMSVSHAVVIATRPAFEAGHELSAALVAAVRKSLETLAALGVHE
                     VAVVDGRHAEELRDRLALHELPLLRVEVIANLSWRNLSGSAVLLAKPWLERSERCLIV
                     RGDRPPELEALRLLTQLERSDADAALAVSLTPEVEPGADVRVKLARQDDTELYAVTSI
                     GEDLVGADGVFTGHALVNRTIIDALSALPNPTLEAGLHELARKGRVVAVTSASWAWST
                     RRPIDVEDNVHALLQAKRHAHYTLLNPGPVNTTATVKSALVHPDVCHRDSVFSELMVS
                     MTNRLRRIYRGTPHHTVVTITGSGTAAMEAALVSTVPSDRKILIIDNGAFGARLVEVA
                     TAHDMDIVHLRYEWGEQVRVEDVERALAQYPEIAVVAMIHHETSVGLLNPVREIGALC
                     KRGDALLVVDAVSSLGAEDIDVVRDNIDVCYASANKCLHAVSGASFVCVAPRVWPRIE
                     ALNPRSYYLDLRRYRRYMDELAQTPFTPAVSVYFALDAACAEFLADGHAARFDMYRER
                     NAKLREGLEALGMPPFTKHGCESSSIVTCRLPEGVAFDALYGAVKARGIVLYGCKGVL
                     ARDYLQIANMGHLADGTIEHLVQVLAEEIARLRSLPKATPVSVIGPAQNERIAG"
     gene            4628..>5321
                     /locus_tag="VFV99_14740"
     CDS             4628..>5321
                     /locus_tag="VFV99_14740"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5060622.1"
                     /translation="MALQKGVNVKPAEGKLGILLPGMGAVATTFIAGVQAIRRGLGKP
                     IGSLTQLGHIRIGKRTDGKSPAIKDYVSIANLDDIAFGGWDIFHDNAYQAAVRAGVLD
                     TRLLDQLKPELEAIKPMTAVFEQKYVKKLNGPNVKKGTSKLDLANQVMDDIKAFKATN
                     KCDRLVAVWCGSTEVYRKPGDVHSTLAKFEAGLERSDDDIAPSQIYAYACLKMGVPYA
                     NGAPNLSVDTPAL"
BASE COUNT          954 a         1709 c         1764 g          894 t
ORIGIN      
        1 cgcaagaagc tcgagacgat cgcgaagcac gcgaagttcc gcgtcacgct caatagcgtg
       61 atcggaagcg cgccggcggg cgagccagcc gaagtcgtcg cgtttgcgaa ggaacacgga
      121 ttcaagccgc gtgtttcgct cgttcatgac ggcaacggtc agttcaagct cgagcccgaa
      181 cagcgcgccg agttcgatca cctcaagcat ttgagtggtg gtggtcggat ggaagccgca
      241 ggcgactatc gcggcaagct catggccgaa gggaaggcac cgttcaagtg tcgttccggc
      301 agccgctatc tctacgtcga cgagttcggc atcgtgcgtt ggtgctcgca gacccgcgac
      361 cgctggggcg taccgctctc cgactattcg cgcgccgagc tcaagcgcca gttcgatacg
      421 aagaaggaat gcaacgaacg ctgcactgtc ggctgtgtcc gtaaggcgag cgggccggac
      481 tcgtggcgcg gccagtcgca acccgatccg ggtgtacctg ccgagcaact cgtgcagatc
      541 ggcaacaagc cgccaagcgc gtctgtgcaa ccgtagggct acgccgccac gtagcgcggt
      601 acgccgccgc gccgcttgat ctcggcgagc tggcggcgca gccgcacatg ctcgggcacg
      661 atgatcgcgc ccgcgacgat cgtgccgagc cagataccgg cgtagatcac gacgtagcca
      721 tcgaagaacg cgacgatcat cgctgcgaga ttgatgaagt cgcgacgaat catgtagagg
      781 aagatctggc cgagcgcctt tgcccacgcc ggcgcggtcg agaccttcac gcgtttgcgc
      841 agcgcaggca ttccgccgtc gtcgacaatc tcgaggtcgc cctcgtagta ctggctgcca
      901 cctttcttga tcacgacgag cgagaagtaa tagatcgcgt agatcgtcgc cgcgtatgaa
      961 atgccgccga gcgcgatcga gccgccgagc caccacgacg gatggtgctg gtaggtgtgg
     1021 tagccgatcg cgaagaagta actgacctgc gttagctcgt cgatcacggt gtcgagccac
     1081 gcaccgaacg gcgagaactg cagcttcatg cgcgcgatct cgccgtcgca gccgtcgatg
     1141 acgcaggacc agaacacgag cacggcgccc cacacgagcg ccgcgtacga cgggaacgcg
     1201 gtcaacacgc agccggcgag gccgagcacg ccgacgaagt acgagacctg gttcggcgtg
     1261 atcgacgtgt gcaagagcaa ccgcgtgagc ggccgcgaga gcgggcggta gacgtacttg
     1321 ctgaccgggc tgtcctcgct cgccttgacg agcagctgca gcagcatccg ttcggcgccg
     1381 cgcttctcct gcggcgtggt cgcgcgatgc accgcgatgt cgtcgtgacg gatgcgctcg
     1441 gcgctctgcc aaccggcgac caggttctgc tcggctgact cgaccgacag cgtgagcgcc
     1501 gcgatgatct cgcggccgtg cgcagcgtcc gcccacagcg cgccggcgta ctcgccggtg
     1561 ggcccgactg cgatccggcg cggatcgagc ccggcgatga gcggccggac gagcggcaga
     1621 tgcacgagct gatcgcctgc gcgcacgaac agaagtgcgg catcaccgcg gtcgcgatcc
     1681 caggcagcga gaccggcgag tgctgcttcg ctgtcgatca tgtacacgcg gcgtgcaccc
     1741 gctttgtcgg cgacgcggcg gccgcgctcg gcgagcgtga tgccaagcag tttgtcgttc
     1801 gccgtcggcg cagtcgcgag caccacggcg tcgaacatgc ggctagccct gggcgaggac
     1861 cgcttcggcg cgtttgagat ccgcgtgatc gtcgatctcg agcacgcggc agtcgtcgac
     1921 cttcacgatg ccgaacggta cctcaccgcg ctgcgtgagg cggtgatacg agtggtcgta
     1981 gtactcgtgc gtgaggttct cggccgggaa cttcgcgagc tcgcggaata ccgcggcgcc
     2041 cgcagtcgcg gagagcttgg tgacaccgat gtattcgccg atgcaatttg cggcgatgag
     2101 cttcttgtcc agggcgtaga cctggttgcc gcgcatctcg actttcatcg catcatcgtc
     2161 gagctccgcg ctggtgtcgg tcgcgagcaa cgcgtcgccg ggagcggcga tcaaacgcgg
     2221 caggatcttg tcgtcgagga tgacgtcacc gtcgagctgg aggaagtcgt ggccccgcag
     2281 gtacggctcg gcgacgagca ccgagtagaa gttgccccat ggctcgtact tctcgttgag
     2341 cacgaccttg cagcgaaagc cgccctcgtc gagagatgcc ttcagttggt cgatccgata
     2401 gccaccgacg acgacgacgt cctcgctggc cacacccgca gccgcgagcc aatcgagctg
     2461 gcgaaatagc agtgatcttc cgagaacggg caccaggacc ttgggccggt cgttggtcag
     2521 gggcgcgagg cgagaaccga cgccagcagc caggatcacc gctttcatga gcgcggcacg
     2581 atacgcgacg ccaggggcca gtcaagcggt gcagaaatcg caccccgggg gacgcgttca
     2641 atagttgacg gaggcgacgg taagtgattg attcggtgac cggttcgcta gttgcaaatc
     2701 gcatttgtgt gacgcgtgaa atgatgtccg tttctcatgc agtggtgatc gcgacgcgtc
     2761 ctgcgttcga agcagggcac gagctttcgg ctgcgcttgt tgcggcggtt cgcaaatcgc
     2821 tcgaaacgct cgcggcgctc ggcgtgcatg aggtggcggt ggtcgacggg cggcacgcag
     2881 aggagcttcg cgatcgtctc gcgctccacg agctgccgct gctgcgcgtc gaagtgattg
     2941 cgaatttgtc gtggcgcaac ctcagcggct cggccgtgct gctcgccaag ccgtggctcg
     3001 agcgcagcga acgctgtttg atcgtgcggg gcgatcgccc gcccgagctc gaagcgctgc
     3061 gcctgctcac gcaactcgaa cgttctgacg ccgacgcggc gctcgccgtg tcgttgactc
     3121 ccgaggtcga gcccggtgcc gacgtgcgcg tcaagctcgc gcgccaggac gacaccgagc
     3181 tctatgcggt cacgtcgatc ggcgaagatc tcgtcggcgc cgacggcgtg ttcacgggcc
     3241 atgcgctcgt caatcgcacg atcatcgacg cactcagcgc gctgcccaat ccgaccctcg
     3301 aggcaggtct gcacgagctc gcgcgcaagg gccgcgttgt cgcggtgacc agcgcatcgt
     3361 gggcgtggtc gacgcgtcgc ccgatcgacg tcgaggacaa cgtccacgcg ctgctccagg
     3421 cgaagcgcca cgcgcactac acgctgctca atccagggcc ggtcaacacg accgcgaccg
     3481 tgaagagcgc gctcgttcac cccgatgttt gtcaccgcga ctcggtgttt tccgagctga
     3541 tggtgtcgat gacgaaccgg ctgcgtcgca tctaccgcgg cacgccgcac cacaccgtcg
     3601 tcacgatcac tggtagcggc accgcggcga tggaagcggc gctggtgtcg acggtgccga
     3661 gcgatcgcaa gattttgatc atcgacaacg gtgcgttcgg cgcccgcctc gtcgaggtcg
     3721 cgacggcgca cgacatggac atcgtccacc tccgctacga gtggggcgag caagttcgcg
     3781 tcgaggacgt cgagcgcgcg ctcgcgcagt atccagagat cgctgtcgtc gcgatgatcc
     3841 atcacgagac ctcggtcggc ttgctcaatc ccgtccgcga gatcggcgcg ctgtgcaaac
     3901 gcggcgacgc gctgctcgtc gtcgatgcgg tgtcgtcgct cggcgctgaa gacatcgacg
     3961 tcgtgcgcga caacatcgac gtgtgttacg cgagcgcgaa caagtgcctc cacgcggtga
     4021 gcggtgcgtc gttcgtgtgc gttgccccgc gcgtttggcc gcgcatcgag gcgctgaacc
     4081 cgcgctcgta ctatctcgac ctccggcgct accgccgcta catggacgag ctcgcgcaga
     4141 cgccgttcac gccggccgtc tcggtgtact tcgcgctcga cgcagcgtgc gcggaattcc
     4201 tagccgacgg ccacgcggcg cggttcgaca tgtaccgcga gcggaacgca aagttgcgcg
     4261 aaggcctcga agcgctcggc atgccgccgt tcacgaagca tggctgcgag tcgagctcga
     4321 tcgtgacgtg ccggctgccc gaaggcgttg cattcgacgc gctttacggc gccgtcaagg
     4381 cgcgcggaat tgtcctgtac ggttgcaagg gcgtgcttgc gcgcgactac ctgcagatcg
     4441 cgaacatggg tcatctcgca gacgggacga tcgagcatct ggttcaggta ttggccgaag
     4501 agatcgcccg tctgcgcagc cttccgaagg caacgcctgt cagcgtgatc ggtcccgcgc
     4561 aaaacgaacg tattgcaggc taattcgaga cttgcgcgtc gacgagcctt gaggcaggtt
     4621 ccccgccatg gctctgcaga aaggcgtcaa cgtcaagccc gccgagggaa agctcggcat
     4681 cttgctcccc ggcatgggtg ccgtggcgac caccttcatc gctggtgttc aggcgatccg
     4741 gcgcggtctt ggcaagccga tcggctcgct gacccagctc ggacacattc gcatcggcaa
     4801 gcgcacggac ggcaagtcgc ccgcgatcaa ggactacgtg tcgatcgcga acctcgacga
     4861 catcgcgttc ggtggctggg acatcttcca cgacaacgcc tatcaagctg cagtacgcgc
     4921 cggcgtgctc gacacccggc tcctcgatca gctcaagccc gagctcgaag cgatcaagcc
     4981 gatgacggcg gtgttcgagc agaagtacgt caagaagctc aatggcccga acgtgaagaa
     5041 gggcacgtcg aagctggacc tcgccaacca ggtgatggac gacatcaagg cgttcaaggc
     5101 cacgaacaag tgcgaccgcc tcgtcgcggt gtggtgcggc tcgaccgagg tctatcgcaa
     5161 gccgggcgac gtgcactcga cgctcgcgaa gttcgaggct ggcctcgagc gttccgatga
     5221 cgacatcgcg ccgtcgcaga tctacgcgta cgcgtgcctc aagatgggcg tgccgtacgc
     5281 gaacggcgca ccgaacctgt cggtcgacac gcccgcgctc c
//
LOCUS       DASUFX010000369         9975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1032572, whole genome shotgun sequence.
ACCESSION   DASUFX010000369 DASUFX010000000
VERSION     DASUFX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032572"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFV99_14745"
     CDS             <1..808
                     /locus_tag="VFV99_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060623.1"
                     /translation="VPPPGQAEFSFDGGREIKGWAVSAGLGFLDRPMRLHTTQIKIFP
                     VEQRETLALGGVYQVGNALIVDARMPFAHQTGDRLMGLGDRTPLQATVVGDLTLGARL
                     RVMERETFATYVRGNLTLPTGADFDFAGESRYMFAWMLIGRWTLPGDVVIAGNGGVRF
                     RPREVVVADRLIGDELFGNLGVSVPLPAIHGLYCDENEVRLTGELVGVLGSDVGGKAG
                     ASPAEVRVGMTERIRPWLAIAARVGKGIDDHIGAPRFRAMVELVYQGGAR"
     gene            821..2644
                     /locus_tag="VFV99_14750"
     CDS             821..2644
                     /locus_tag="VFV99_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060624.1"
                     /translation="MVAAPARFAPADRPRIAVAADGTLAAIHERTRVVVTEVPSCAQF
                     AELGVDAKASDAEVVWVGTPPRLLVVSRYAAHSTVHLVDPFGPRTIAELRFESPMQLR
                     ASVGNFALAIGAQGAMVLAATDRGLTPYQFPARAIPTTAGAAGTQFVVALPGAIEEWD
                     PQSRLPKRRLKLPRPAQITAVGGSDRAVWMTTQQDPTRIDVLPFVNRGQPKHHELPEP
                     IGHVASHPRSDTIACIGATTGRVYVIDLDGHTGMRTVGPEGIEQVEGVGLVVGRVTGL
                     LAAQTKRPLVVVPLEHHEEPVVAAPVLPRSERSESIKRSSLYGDDEQNTNVTLSSPRA
                     EPAKPAEPAEPAEPAKPAEPAKLAEPKLAEPKLAPDKAALASFRQRVGGNRRTLQPFS
                     LWAEAPQEWRGEIAVWTYDVLANQPVSDTPQSVPIDTLITRYDLMEAFVPVLTVLYGA
                     HLVGANGAAPIDIARFVGWPEALGRGDLADKRIAVFAESRVRLAPALLRVLDELPPTT
                     GTLIGTPGVVSLLGPCTLVSAGPLTIIAEACGSSISGAILAAHDNADPHELVAEARAY
                     GAAPMLRVSPRTLATVPVDQPVILVADDDHTADQLGLPRLT"
     gene            complement(2641..4116)
                     /locus_tag="VFV99_14755"
     CDS             complement(2641..4116)
                     /locus_tag="VFV99_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060625.1"
                     /translation="MPSNTYDLVVLGDDIAALVCATLCARRGMRTLVLGSDRPARYML
                     GPHKLPIEPMLWPVGTGSPGERVLKELHAEMALRRKLREPRIAAQIVAPDFRVDLGSD
                     RVASELARELGAASDALLTRWQRGRDIARTLDPLFASEHAYPGVGFFERRETTKLAEQ
                     ATEAAQAWWADCETDPNAKLWRHLGAIALRHPNAPAMALTRALDAWHAGPPALRGDGE
                     AVRELLLEKLTTAGGELRAGTVTEANVSWSKLASVTLQNGDEIGAGQVVASKTASELA
                     ELLGKKTPKKVSEMAESMQLVGYRYTFNIVMAEAGIPEHMAPTVAVIADPDKDPVGDA
                     AFSIHLGETDDGGRVVATVAAVLASTELLDADRAAPKMAALRAGLWKRLGDVMPFFEK
                     HLVLAHSPFEANPPHVPGGRGSYDVPRNVPLPMPPVWQDNPVATYVTGLKNLTLTGDQ
                     ILPGYGIEGALVSGWSAAKIACALAGKKKDYLRDEVVGAAS"
     gene            complement(4106..4714)
                     /gene="purN"
                     /locus_tag="VFV99_14760"
     CDS             complement(4106..4714)
                     /gene="purN"
                     /locus_tag="VFV99_14760"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5060626.1"
                     /translation="MKIGVLVSGSGTNLQALLDAEARGELAPGTIAVVVSNRPEAPAL
                     QRATDAGKPAVVVDHKQFAERQAFEDKLLATLDEHGAELVVLAGFMRVLTPHFITRYP
                     QRIINTHPSLLPAFPGVDAAAQAIAHGVKVSGVTIHFVDTSLDGGPIITQVPVAVFPA
                     DDAGSLQARIQREEHRVLADVVRRLAAGLYSCQGRHVVERAE"
     gene            complement(4711..5736)
                     /gene="purM"
                     /locus_tag="VFV99_14765"
     CDS             complement(4711..5736)
                     /gene="purM"
                     /locus_tag="VFV99_14765"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5060627.1"
                     /translation="MALTYKDAGVDIDAGNALVERIKPLAKATTRPEVLGGIGGFSAL
                     CRIPTAYKEPILVSGTDGVGTKLKTAFAANRHDTIGIDLVAMCVNDVLVTGAEPLFFL
                     DYFATGALDVDTAASVISGIAEGCRQSGCALVGGETAELPGLYHGSDYDLAGFCVGVV
                     EKSAIRPRRDLVAGDIVIGLPSAGLHSNGHSLARKVVLETLKLPLDAKPDVLGGATVA
                     DEMLRPTIIYADAFKRIAEQPWKAAAHITGGGLVENPPRIFADDTLAVELDPSTWEVP
                     AILKLIQSAGVTDDEMNRTFNMGLGMIIVVPADAAARSVELLASYRARVVGKLVPRGG
                     GESSRFK"
     gene            5760..6392
                     /locus_tag="VFV99_14770"
     CDS             5760..6392
                     /locus_tag="VFV99_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060628.1"
                     /translation="MPVIDVVVPVRDHAASLGPLLRELPHRAIRSTVVVDRASKDRSA
                     EIARDAGALVLREAGGGYGAACLRAQAHFGALPRVPDIVVFVPGDRPAAAAAVLDLVA
                     PIVDRSVELVVGHETGRRKVREKLVLGLIDTVYRQRWSGVGPVRALKFPALVALGMRD
                     RGDGWDVEMLVRAVKLGLTCDEVGLPADAHRDPKVGSRALLHILRHATMR"
     gene            6465..7139
                     /locus_tag="VFV99_14775"
     CDS             6465..7139
                     /locus_tag="VFV99_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5060629.1"
                     /translation="MNDGLERFARDFKAGTVLFEEGQPGADMYVVLAGQVEIRRKVGE
                     IEHALAVLMPGDFFGEMAILNARPRSATAVTRIDSRLLVIEGRMFEAMLRARPEIALR
                     IIKALAMRLDNSNQHVELLLLPTPNHKVVQCLRHMAEEQMMLAGGQVTPGTAILVPKR
                     VEDIAVRVGLPVAEVIDVVDRLRAARLLLLAQDAGIDGDGFIVPEVGRLLEFLEFLNL
                     KDRFGV"
     gene            complement(7170..7478)
                     /locus_tag="VFV99_14780"
     CDS             complement(7170..7478)
                     /locus_tag="VFV99_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060630.1"
                     /translation="MSSVLVVNEISTEREEITRALEAEGFTVVQANSAAEAVREIWGG
                     SFIVAIVSTLLTGTTSTALVQQLGQMAPEIETLVHGKNDELPALVRKVISIRDGEAAA
                     "
     gene            7688..8593
                     /gene="accD"
                     /locus_tag="VFV99_14785"
     CDS             7688..8593
                     /gene="accD"
                     /locus_tag="VFV99_14785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5060631.1"
                     /translation="MAWWSRTQGLAEQPKKSVPKGIWIKCERCAATLYEADLDANFRV
                     CSSCQHHFRMPTEARIKMMCDLGTWQEHDRELESGDPLGFRVDGKKYVDQVKGTTKKV
                     GPGDAYRAGTATMGGIAVEIGFFVFEFMGGSMGSVVGEKITRQFERAAAHKRPAIMFS
                     ASGGARMQEGVLSLMQMAKTSAARSRLREARVPYISVLLHPTTGGVAASVAMLGDLIV
                     AEPDALIGFAGPRVIEQTIRQQLPPGFQRSEFLLEHGMVDMVVPRKDLKSTLERTLRW
                     FERGREPERRTGTAADDIAEPPATD"
     gene            8639..9868
                     /locus_tag="VFV99_14790"
     CDS             8639..9868
                     /locus_tag="VFV99_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5060632.1"
                     /translation="MTYVELLQRLVSARRFGMKLGLERMRELLDTLGAPDRKLGTVVH
                     IAGTNGKGSTVAMVAALARSAGKRVATYTSPHLSTLRERITIDGQLVSEDEVVRAAEE
                     VSAAGGDELTFFEQVTAIAFVIIAARAVDITVLEVGLGGRLDATNVVAAPIAAVTGVA
                     LDHEAILGTTLDAIAFEKAGIFKRGQRAFIGCAGEPAAVPLLVQHARAAGVAELSVID
                     EPANVTLALPGAHQQQNAALALALVEALGLAPDRSVLATLSHPGRFEIVGDVILDGAH
                     NPHGARALAATLQERGLRPTLVVAVSADKDVAAIASALEPQVSAVIATRYQQERALAP
                     EDLANGFRAAVGQGGAQAMQIETAPDLATAIARARTLGSPILIAGSLFLVGEARTLLL
                     GAPTDPVVVSDPVSARA"
BASE COUNT         1579 a         3355 c         3396 g         1645 t
ORIGIN      
        1 cgtgccgccg ccgggacagg cggagttcag cttcgatggc ggccgcgaga tcaagggctg
       61 ggcggtgagc gccgggctcg gcttcctcga tcggccgatg cggctgcaca cgacccagat
      121 caagatcttt cccgtcgaac agcgcgagac cctcgcgctc ggcggcgtgt accaggtcgg
      181 caacgcactg atcgtcgatg cgcggatgcc gttcgcgcat cagaccggcg accgcttgat
      241 gggcctcggc gatcggacgc cgctccaggc gactgttgtc ggtgacctca ccctcggcgc
      301 ccggctgcgc gtgatggaac gcgagacgtt cgcgacgtat gtgcgcggca acctcacgct
      361 gcccaccggc gctgacttcg acttcgcagg tgagtcccgc tacatgttcg cgtggatgct
      421 gatcggccgg tggacgctcc ccggcgacgt cgtgatcgcc gggaacggcg gcgtgcggtt
      481 tcgtccgcgc gaggtcgtcg tcgcggaccg tctcatcggc gacgagctgt tcggcaacct
      541 tggcgtgagc gttccgctcc ccgcgatcca cggcctctac tgcgacgaga acgaagtccg
      601 tctcaccggc gagctcgtcg gcgtgctcgg cagcgacgtc ggcggcaaag cgggcgcgtc
      661 gccggcggag gtgcgagtag gcatgaccga gcgaattcgc ccatggctcg caattgctgc
      721 gcgcgtcggc aagggcatcg acgatcacat cggcgcacct cggttccgcg caatggtcga
      781 gctcgtctac cagggcggcg ctcggtgata cgatcgttcg gtggtggcag ctccggcgag
      841 gtttgcgccg gccgatcgac cgcgaattgc ggtcgcggct gacggcacgc tcgcggccat
      901 tcacgagcgc acgcgcgtcg tcgtcaccga ggtgccgagc tgtgcgcagt tcgccgagct
      961 aggcgtcgat gccaaggcaa gcgacgccga ggttgtctgg gtcggtacgc cgccgcgcct
     1021 cctcgtcgtg tcgcgctatg cggcgcacag cacggttcac ctcgtcgatc catttggtcc
     1081 gcgcacgatc gctgagctgc ggttcgagtc gccgatgcag ctccgcgcaa gcgtcggcaa
     1141 cttcgcactc gcaatcggcg cgcaaggcgc gatggtgctg gccgcgaccg atcgcggcct
     1201 gacgccatac cagtttcccg ctcgcgcgat tccgacgacc gcgggcgcag ccggcaccca
     1261 gttcgtcgtc gcattgccgg gcgcgatcga ggagtgggat ccgcagagcc ggctgccgaa
     1321 gcgccggctc aagctgccgc gtccggcgca gatcacggcg gtcggcggta gcgatcgcgc
     1381 cgtgtggatg acgacgcagc aagacccaac gcggatcgat gtcctcccgt tcgtcaatcg
     1441 cggtcagccc aagcatcacg aattgcccga accgatcggt cacgtcgctt cgcatccgcg
     1501 cagcgacacg atcgcgtgta tcggcgcgac caccggccgc gtctacgtca tcgatctcga
     1561 cggccatacc ggcatgcgca cagtcggccc cgaaggtatc gaacaagtcg aaggcgtcgg
     1621 cctcgtcgtc ggcagagtca ccggcctcct cgccgcacag acgaaacgcc cgctcgtcgt
     1681 cgtaccgctc gagcaccacg aagagcccgt agtagccgcg ccggtgttgc cgcgcagcga
     1741 gcgcagcgag tcaatcaagc gctcgtcgct gtacggcgat gatgagcaaa acacgaacgt
     1801 cacgttgagc tcgccgcgcg ccgagccggc aaaaccagcc gagccggccg aaccagctga
     1861 gccagccaaa ccagccgagc cagcgaaact ggccgagccg aaactggccg agccgaagct
     1921 ggccccggat aaggccgcgc tcgccagctt ccgccagcgc gtcggcggca accggcggac
     1981 gctccagccg ttctcgctct gggccgaggc accgcaggag tggcgcggcg aaatcgcagt
     2041 gtggacctac gacgtcctcg cgaaccagcc ggtgtccgac acaccgcagt cggtgccgat
     2101 cgacacgctg atcacgcgct acgatctgat ggaggcgttc gtaccggtgc tcaccgtcct
     2161 ctacggcgcg cacttggtcg gcgcgaacgg cgcagcaccg atcgacatcg cgcgtttcgt
     2221 cggctggccc gaggcgctcg gtcgcggcga cctcgcggac aagcgcatcg cggtgttcgc
     2281 cgagtcgcgt gtgcggctcg cgcctgcact gctgcgcgtg ctcgacgagc taccaccgac
     2341 aaccggcacg ctcatcggta cgccgggcgt cgtatcgctg ctcggcccgt gcacgctcgt
     2401 atcggccggc ccgctcacga tcatcgccga ggcgtgtggc tcgtcgatca gcggcgcgat
     2461 tctcgccgcg cacgataacg ccgatccgca cgagctcgtc gccgaagccc gcgcgtacgg
     2521 cgcagccccg atgttgcgcg tcagcccgcg cacgctcgcg accgtgcccg tcgatcagcc
     2581 ggtcattctc gtcgccgacg acgatcacac cgccgatcag ctcggactgc cgcgcctgac
     2641 ctagctcgcc gcgccgacaa cttcgtcgcg caggtagtcc ttcttcttgc cggcgagcgc
     2701 gcacgcgatt ttcgcggcgc tccagccgga gacgagcgcg ccttcgatgc catagccggg
     2761 caggatctga tcgcccgtga gcgtgaggtt cttgagcccg gtcacgtacg tcgccaccgg
     2821 gttgtcctgc cacaccggcg gcatcggcag cggcacgttg cgcggcacgt cgtagctgcc
     2881 gcggccaccc ggcacgtgcg gcgggtttgc ttcgaagggc gagtgcgcga gcacgaggtg
     2941 cttctcgaag aacggcatca cgtcaccgag tcgcttccac agccccgcgc gcagcgccgc
     3001 cattttcggt gcggcgcggt cggcatcgag gagctcggtg ctcgccagga ctgccgcgac
     3061 ggttgcgacc acgcggccgc cgtcgtctgt ttcgccgagg tggatcgaga acgccgcatc
     3121 accgacggga tccttgtcgg gatccgcgat caccgcgacc gtcggcgcca tgtgctcggg
     3181 gatgccagct tcggccatca cgatgttgaa cgtgtagcga tagccgacga gctgcatgct
     3241 ctcggccatc tcgctgacct tcttcggcgt cttcttgccg agcagctcgg cgagctcgct
     3301 ggcggtcttg ctcgcgacga cctggccggc gccgatctcg tcgccgttct gcagcgtgac
     3361 gctcgcgagc ttgctccacg acacgttcgc ctcggtcacc gtcccggcac gcagctcgcc
     3421 gcctgccgtc gtgagcttct cgagcagcag ctcgcggact gcttcgccgt cgccgcgcaa
     3481 cgcaggcgga ccggcgtgcc atgcgtcgag cgcgcgggtc agcgccattg caggtgcatt
     3541 cggatgccga agcgcgatcg cgccgaggtg gcgccacagc ttcgcgttgg ggtcggtttc
     3601 gcaatccgcc caccaggcct gagcagcctc ggtggcttgc tcggcgagct tcgtggtctc
     3661 gcggcgctcg aagaatccaa cgccgggata ggcgtgctcg ctcgcgaaca gcggatcgag
     3721 cgtgcgtgcg atgtcgcggc cgcgctgcca gcgcgtgagc agcgcatcgc ttgctgcacc
     3781 gagctcgcgc gccagctcgc tggcgacacg atcgctcccg agatcgacgc gaaaatccgg
     3841 cgcgacgatc tgcgccgcga tgcgcggctc gcgcagcttg cgtcgtagag ccatctcggc
     3901 gtggagctct ttgagcacgc gctcgccggg tgacccggtg ccgaccggcc acagcatggg
     3961 ctcgatcggg agcttgtggg ggccgagcat gtagcgcgcg ggccgatccg agccgagcac
     4021 gagcgtgcgc atgccacggc gcgcgcacag cgtggcgcag accagcgcgg caatgtcgtc
     4081 acccagtacg accagatcgt acgtgttact cggcacgctc caccacgtgc cgaccttggc
     4141 acgagtagag acccgccgcc aagcgccgaa ccacgtctgc aagcacgcgg tgctcctcgc
     4201 gctgaattcg cgcctgcagc gatccagcgt catcggccgg aaacaccgcg accggcactt
     4261 gcgtgatgat cggtccgccg tcgaggctcg tgtcgacgaa gtgaatcgtc acgcccgaca
     4321 ccttcacgcc gtgcgcgatt gcttgcgccg ccgcgtcgac accggggaac gcgggcaata
     4381 gcgacgggtg cgtgttgatg atccgctgcg gatagcgcgt gatgaagtgc ggcgtgagca
     4441 cgcgcatgaa gccagcgagc acgacgagct cggcgccatg ctcgtcgagc gtcgcgagca
     4501 gcttgtcctc gaatgcctgg cgctcggcaa actgcttgtg atcgacgacg accgccggct
     4561 tgcccgcatc ggtcgcgcgc tgcagggcag gggcctcggg gcgattcgag acgacgaccg
     4621 cgatcgtgcc aggtgcgagc tcgccgcgcg cttcggcatc gagcagcgcc tgcagattcg
     4681 tcccgctgcc ggagacgaga acaccgatct tcatttgaac cgcgatgatt cgccgccgcc
     4741 gcgcggcacc agcttgccga cgacgcgcgc gcgatagctc gcgaggagct cgacactgcg
     4801 cgcagccgca tccgctggca cgacgatgat catgccgagg cccatgttga acgtgcgatt
     4861 catctcgtcg tcggtgacac cggcgctctg gatcagtttc aggatcgccg gcacctccca
     4921 cgtcgatggg tcgagctcga ccgcgagcgt gtcgtccgcg aagatgcgcg gcggattttc
     4981 gacgaggcca ccaccggtga tgtgcgcggc cgccttccac ggctgctcgg cgatgcgctt
     5041 gaacgcgtcg gcgtagatga tcgtcgggcg cagcatctcg tcggccaccg tggcgccgcc
     5101 gagcacgtcc ggctttgcat cgagcggcag cttcagcgtc tcgagcacga ccttgcgcgc
     5161 gagcgaatgg ccgttcgagt gcaggcccgc ggacgggagg ccgatcacga tgtcgccggc
     5221 cacgagatcg cggcgcgggc gaatcgcact cttctcgacg acgccgacgc aaaagccggc
     5281 gaggtcgtag tcgctgccgt gatagaggcc gggcagctcg gcggtctccc cgccgacgag
     5341 cgcgcatccg gactgccggc agccctcggc gatgccgctg atcacgctcg ccgcggtatc
     5401 gacatcgagc gcgccggtcg cgaagtagtc gaggaagaac agcggctcgg cgccggtgac
     5461 gagcacgtcg ttgacgcaca tcgcgaccag gtcgatgccg atcgtgtcgt ggcggttcgc
     5521 cgcgaacgcg gtcttgagct tggttccgac gccatcggta ccgctgacga ggatgggctc
     5581 cttgtacgcg gtcggaatcc ggcacagtgc actgaatccg cctatgccgc cgagtacctc
     5641 ggggcgcgta gtcgccttcg cgagcggctt gatgcgctcg acgagtgcat tcccggcgtc
     5701 gatgtcgacc cctgcgtcct tataggtgag cgccatcgcc gctcgtgtac cataccccgg
     5761 tgcctgtcat cgacgtcgtg gtgccggtac gcgaccacgc agcgagctta ggaccactgc
     5821 tacgcgagct cccgcatcgg gcgattcgtt cgacggtcgt ggtcgatcgc gcgtcgaagg
     5881 accgcagcgc cgagattgcg cgcgatgccg gcgccctcgt gctgcgcgag gcgggcggtg
     5941 gctacggtgc cgcttgtctg cgggcgcagg cgcatttcgg agcgctgcca cgcgtccccg
     6001 acatcgtcgt gttcgtgccc ggagatcggc cagccgcggc agctgcggtg ctcgacctcg
     6061 tggcgccgat cgtcgatcgc agcgtcgagc tggtcgtcgg tcacgagacc gggcggcgca
     6121 aggtccgcga gaaactcgtt ctcgggctga tcgacaccgt ctatcgccag cggtggtcgg
     6181 gtgtgggccc ggtgcgcgcg ctcaagtttc ctgcgctggt cgcgctggga atgcgtgatc
     6241 gcggcgatgg ctgggatgtc gagatgctgg tccgcgcggt caagctaggc ctgacctgcg
     6301 acgaagttgg gcttcccgcc gacgcacatc gcgatcccaa ggtgggaagt cgcgcgctac
     6361 tccacatcct gcgccacgcg acgatgcgat gatcgccgcg acgatggtcg tggcgaggat
     6421 ggtcgcaact tggccggctg agatctccgt gggatgatgg tgctgtgaac gacggcttgg
     6481 agcggtttgc acgcgacttc aaggcgggca ccgtgctgtt cgaagagggc cagcctgggg
     6541 cggatatgta cgtcgtgctg gcggggcagg tggagattcg ccgcaaggtc ggcgaaatcg
     6601 agcatgcgct cgcggtgttg atgccgggcg atttcttcgg ggagatggcg atcctcaacg
     6661 cgcggccgcg ctcggcgacc gcggtcacgc gcatcgattc gcggctgctc gtgatcgagg
     6721 ggcgcatgtt cgaagcgatg ctccgcgcgc gtcctgaaat cgcgctgcgc atcatcaagg
     6781 cgctcgcgat gcggctcgat aattcgaacc agcacgtcga gctgcttctg ctgccgacgc
     6841 cgaaccacaa ggtcgtgcag tgcctgcggc acatggccga ggagcagatg atgctcgccg
     6901 gcgggcaggt cacgccgggc accgcgatcc tggtgccgaa gcgcgtcgag gacattgccg
     6961 tacgcgtcgg tctgccagtc gccgaggtga tcgacgtcgt cgatcgtttg cgtgccgcgc
     7021 gcctcctgct gctggcacaa gacgccggca tcgatggcga tggcttcatc gtcccagagg
     7081 tgggacggct actcgagttc ctcgagttct tgaacctgaa agatcgcttc ggcgtttaag
     7141 cagagcggcg aaaagggggc agaccccttt tacgcagccg cttcgccatc acgaatcgag
     7201 atgaccttgc gaacgagagc agggagctcg tcgttcttgc cgtgcaccaa ggtctcgatc
     7261 tcgggagcca tctgaccgag ctgctggacc agagccgtgc tggtggtacc cgtgagcagc
     7321 gtcgacacga ttgcgacgat gaacgagcca ccccagattt cccggactgc ctcggccgca
     7381 gaattggcct ggacgaccgt gaagccttcg gcttcgaggg cgcgggtaat ttcctcgcgc
     7441 tccgtgctga tttcattaac gactaggacg gaagacatcg cgacctcact aggggcggcc
     7501 gaattctatc gatctacacc caaaacgccc ttcaggctgc gttttccctt ttggtgtcta
     7561 gcagaagcgt ggccataccg cgagagaaag tcgatggaat cacgaggtaa gaaggatttg
     7621 gagatcgtga cgaacctcaa aaccgacccc tttacgcaac ttagcggtcc tgctatgagt
     7681 gcgccccatg gcctggtggt cgcgaacgca ggggctggct gagcagccga aaaagtccgt
     7741 ccccaaaggg atttggatta agtgcgagcg gtgcgcggcc accctgtacg aggccgacct
     7801 ggacgctaac ttccgagttt gctccagttg tcagcaccac ttccggatgc cgaccgaggc
     7861 gcgcatcaag atgatgtgcg atctcggtac ctggcaggag cacgatcgcg agctcgagag
     7921 cggcgatcca ctcggcttcc gggtcgacgg caagaagtac gtagatcagg tcaagggcac
     7981 gaccaagaag gtcggccccg gcgacgccta ccgcgcgggc accgcgacga tgggtggcat
     8041 cgcggtcgag atcggcttct tcgtgttcga gttcatgggc ggctcgatgg gctcggtggt
     8101 tggcgagaag atcacgcgcc agttcgagcg cgcggccgcc cacaagcgac ccgcgatcat
     8161 gttctccgcg tcgggcggcg cgcgcatgca agagggcgtg ctgtcgctga tgcagatggc
     8221 gaagacgagc gcggcccggt cgcgactccg cgaagcccgc gtgccgtaca tctcggtgct
     8281 gctgcacccg accacgggcg gcgtcgctgc atcggtcgcg atgctcggcg atctgatcgt
     8341 cgcggagccc gatgcattga tcggctttgc cggcccgcgc gtgatcgaac agacgatccg
     8401 ccagcagctg ccgcccggtt tccagcgctc cgagttcttg ctcgagcacg gcatggtcga
     8461 catggtcgtg ccgcgcaagg atctcaagag cacgctcgag cgcacgctgc gctggttcga
     8521 gcgcggccgc gagccggagc gccgcaccgg caccgccgcc gatgacatcg cagagccgcc
     8581 ggcaacggac taaggtccgt cccgcggatc tatgtcacgg ggcggcagcg gattgaccat
     8641 gacgtatgtc gagctgctgc agcggctcgt cagtgcgcgt cgcttcggca tgaagctcgg
     8701 cctcgagcgc atgcgcgagc tgctcgacac gctcggcgcg cccgatcgca agctcggcac
     8761 ggtcgtccac attgccggca cgaacggcaa gggctcgacc gtcgcgatgg tcgctgccct
     8821 cgcgcgctct gcgggcaagc gggtcgcgac gtacacgtcg cctcatctct cgaccctgcg
     8881 cgagcggatc acgatcgatg gccagctcgt ctccgaggac gaggtcgtgc gtgcagccga
     8941 ggaggtgagt gcggcaggtg gcgacgagct gacgttcttc gagcaggtca ccgcgatcgc
     9001 cttcgtgatc atcgcggcgc gcgcggtcga tatcaccgtg ctcgaagtcg ggctcggcgg
     9061 tcggctcgat gcgacgaacg tggtcgcggc accgatcgca gcggtcactg gcgtcgcgct
     9121 cgatcacgag gcgatcctcg gaaccacgct cgacgcgatc gcgttcgaga aggccggcat
     9181 cttcaaacgc ggccagcgcg cgttcatcgg ctgcgccggg gagccggcgg cggtgccact
     9241 actcgtacag cacgcgcgcg cggccggggt ggccgagcta tccgtgatcg acgagcccgc
     9301 gaacgtgacg ctcgcgctgc cgggtgcgca tcagcagcag aacgctgcgc tcgcactcgc
     9361 gctcgtcgag gcgctcggcc tcgcgccaga tcgcagcgtg ctcgcgacgc tgtcacatcc
     9421 cggccggttc gagatcgtgg gcgatgtgat cctcgacggc gcgcacaatc cgcatggcgc
     9481 gcgcgcactc gccgcgacgc tgcaggagcg cggcctgcgg ccaacgctgg tcgttgccgt
     9541 gtcggccgac aaggatgtcg ccgcgatcgc cagcgcgctc gaaccgcagg tgagtgcggt
     9601 gatcgcgacg cgctaccaac aagagcgggc gctcgcgccg gaggacctcg caaatggatt
     9661 ccgagcggcg gtcgggcagg gcggtgcaca agcgatgcag atcgagaccg cgcccgatct
     9721 cgccactgca atcgcacgcg cgcgcacgct cggctcgccg atcctgatcg ccggctcgct
     9781 gtttctcgtc ggcgaagcgc gcacgctgct actcggcgcg ccgaccgacc cagtggtcgt
     9841 cagcgacccc gtttcggctc gggcatagtc cagcgtaggt cgagcagcgt cttctgcgcg
     9901 ccctcccatg acttggcgcg gaacgtggcg cggatctcct tcgcacctgc gagctcgagc
     9961 agaccctcga agaag
//
LOCUS       DASUFX010000370         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_199274,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000370 DASUFX010000000
VERSION     DASUFX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199274"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..772)
                     /gene="cobS"
                     /locus_tag="VFV99_14795"
     CDS             complement(38..772)
                     /gene="cobS"
                     /locus_tag="VFV99_14795"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX5060633.1"
                     /translation="MSLRSFAVALTFLTRTPINLREVRDADFGQSLAWFPTVGLLLGA
                     MLAWGEHLARGHASHEIIALGLVALMLGLTGGLHIDGVADVFDGLSGSRGNRERMLEI
                     MRDSRIGAHGAAAIVIVLVAKVFALVALQRAAISWALFVFPAAARWAACVLLVGFPYA
                     RKEGLGKAFNGHARVSHLVVATCTTVAIIVVVGVRALVPTLAALAVALGLGFWIYRRL
                     GGLTGDVYGAAIELAELAFLIAATTS"
     gene            complement(777..1835)
                     /gene="cobT"
                     /locus_tag="VFV99_14800"
     CDS             complement(777..1835)
                     /gene="cobT"
                     /locus_tag="VFV99_14800"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5060634.1"
                     /translation="MKLVNETCQRIVAIDQHIADETQRILDVKTKPRRSLGRLEDVAC
                     QVAAIRGTTFPATSRKAIVVMGADHGVAEAGVSAYPQEVTAQMLLNFARGGAAINVLG
                     RHADARVIVVDMGVKQPLPADAGIRSERIGPGTRNFAREPAMTRAEAIAAIEVGIRIA
                     IELAAEGVTLIGIGEMGIGNTTAASALTSVFTGASPDEVTGRGTGIDDATFSRKIATI
                     KQALAVTKPDAADAIDTLAKLGGFEIAGLCGVVLGAAAQRVPVVMDGFIASTAALCAV
                     RIAPASAGYLIASHRSVEAGHRLVLAAIGAKPLLDLDLRLGEGSGAALAMSLVDASLR
                     ILSEMATFASAGVSDSGA"
     gene            complement(1819..2385)
                     /gene="cobU"
                     /locus_tag="VFV99_14805"
     CDS             complement(1819..2385)
                     /gene="cobU"
                     /locus_tag="VFV99_14805"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5060635.1"
                     /translation="MTNQLILVGGGVRSGKSTFALARARSLGARRVFVATAQAFDDEM
                     RDRIALHRNERGDEFRTIEEPLAVAQALAALDGVDVVLVDCLTLWLSNLLLREMSTLQ
                     IFERVDELVAVLAQRRFHSVIVTNEVGMGIVPESALGRAFRDIAGIAHQRIARHADEI
                     DVAILGTVLRIKPAPVQIQEGEARETSK"
     gene            complement(2382..3170)
                     /gene="cbiR"
                     /locus_tag="VFV99_14810"
     CDS             complement(2382..3170)
                     /gene="cbiR"
                     /locus_tag="VFV99_14810"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HEX5060636.1"
                     /translation="MMNVPFRLGTTSFIHPGGWAFNVERLAGRVEDVEILFFESDGLP
                     GADEARHLAEHKARTGITYSLHTPLDASLASANESRRQASVSSVRRALEVAIPFRPEV
                     AIVHVYLGEHEHDPNIPSDLDAWRARARLSLESILASGIAPRDLCVEVLDYDFALIEP
                     VIVDLGLSVALDVGHLVRDGADELALLRRHLHRTRVIQWHGLDPMGRDHRGLQYYPRD
                     RARMLIDTLFAEGYDGVLTLEVFREADLDVSLAIVTSLLEDRPS"
     regulatory      3234..3435
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          640 a         1162 c         1166 g          555 t
ORIGIN      
        1 gacgatcgag ccgcgtgcca ggcgcgcgcg ctgagactca gctcgtcgtc gccgcgatca
       61 gaaatgcgag ctcggccagc tcgatcgcgg caccgtagac gtcaccggtc agtccgccga
      121 ggcgacggta gatccagaac ccgagaccga gcgcgaccgc gagtgcggcc agcgtgggca
      181 cgagcgctcg aacgccgacg accacgatga tcgcaaccgt cgtgcacgtg gcgacgacga
      241 gatgcgacac gcgcgcgtga ccattgaacg ctttgccgag gccttccttg cgcgcgtacg
      301 gaaacccgac gagcagcacg cacgctgccc agcgcgccgc cgccgggaat acgaacagcg
      361 cccagctgat cgcggctcgc tggagcgcga caagcgcgaa taccttcgcg acgagcacga
      421 tgacgatcgc cgcggcgcca tgcgcgccga tgcggctatc gcgcatgatc tcgagcatcc
      481 gctcgcggtt gccgcgcgag ccgctcaggc cgtcgaacac gtcggccaca ccgtcgatgt
      541 gcaggccgcc ggtgagaccc agcatcagcg cgacgaggcc gagcgcgatg atctcgtgcg
      601 acgcatgacc acgcgcgagg tgctcgcccc atgcgagcat cgctccgagc agcaggccga
      661 ccgtcggaaa ccaggcgagc gactggccga agtcggcgtc gcgtacctcg cgcaggttga
      721 tcggcgtgcg cgtgaggaat gtcagcgcta ccgcgaacga tcggaggctc actcgatcac
      781 gcccccgagt cgctcacgcc cgcggaggcg aacgtcgcca tctcgctcag gatccgcagc
      841 gacgcgtcga cgagactcat cgcgagcgca gcgccggatc cttcgccgag ccgaagatcg
      901 agatcgagca gcggcttcgc gccgatcgcg gcgagcacga gccggtgtcc cgcctcgacc
      961 gagcgatgcg atgcgatcag atagccggcc gatgccggcg cgatgcgcac cgcgcacaac
     1021 gccgcagtgc tcgcgatgaa gccgtccatc acgacgggaa ctcgctgcgc ggccgcgccg
     1081 agcacgacgc cacaaagccc cgcgatttcg aacccgccga gcttcgcgag cgtgtcgatt
     1141 gcatcggccg catcgggttt ggtgacggcg agtgcttgct tgatcgtggc gatcttgcgc
     1201 gagaacgtcg cgtcgtcgat tccggtgccg cgaccggtga cctcgtccgg cgatgccccc
     1261 gtgaacacgg aagtcagcgc gctcgctgcg gtcgtgttgc cgatgcccat ctcgccgatg
     1321 ccgatcagcg tcacgccttc ggcggcgagc tcgatcgcga tgcgaattcc gacttcgatg
     1381 gccgcgatcg cttcggcgcg cgtcattgcg ggctcgcgcg cgaagttgcg agtgcccggt
     1441 ccgatgcgtt cgctgcgaat gccggcgtcg gcgggcaacg gctgcttgac tcccatgtcg
     1501 acgacgatga cgcgcgcatc ggcatgccgg ccgagcacgt tgatcgcggc gccaccgcgc
     1561 gcgaagttga gcagcatctg cgcggtgacc tcttgcgggt acgcgctgac accggcttcg
     1621 gccacaccgt gatcagcgcc catcacgacg atcgcttttc gcgatgtagc gggaaacgtc
     1681 gtgccgcgga tcgcagcgac ctggcacgcg acgtcttcga gtcggccgag gctgcggcgc
     1741 ggcttggtct tgacgtcgag gatccgctgc gtctcgtcgg cgatgtgctg gtcgatcgcg
     1801 acgatgcgct gacacgtctc atttactagt ttcacgagct tctccctctt ggatctgaac
     1861 cggcgcgggc ttgatgcgca gcacggtacc gagaatcgcc acgtcgatct cgtcggcgtg
     1921 acgcgcgatt cgctgatgcg cgatccccgc gatgtcgcga aacgcgcgac cgagcgccga
     1981 ctccgggacg attcccatgc cgacctcatt cgtcacgatg acggagtgga accggcgctg
     2041 cgcgagcacc gcgacgagct cgtcgacgcg ctcgaagatc tgcagcgtcg acatctcccg
     2101 cagcaacagg ttcgacagcc agagcgtcag acaatcgacg agcacgacat ccacgccatc
     2161 gagcgcggca agcgcctgcg cgaccgcgag tggctcctcg atcgtccgga actcgtcgcc
     2221 gcgctcgttg cgatgaagtg caatgcggtc gcgcatctcg tcatcgaatg cttgcgccgt
     2281 cgcgacgaac acgcgccgtg ctccgagtga acgtgcacgc gccagtgcga acgtgctctt
     2341 gccggagcgc acgccgccgc cgacgaggat cagctggttc gtcacgacgg ccgatcctcg
     2401 agtagcgagg tcacgatcgc gagcgacacg tcgaggtctg cctcgcggaa cacttcgagc
     2461 gtgagcacgc cgtcgtagcc ctcggcaaac agcgtgtcga tcagcatgcg cgcgcggtcg
     2521 cgcggatagt actggagccc gcgatgatcg cggcccatcg gatcgagccc gtgccactgg
     2581 atgacgcgcg tgcggtggag atgacggcgc aacagcgcga gctcgtcagc gccatctctc
     2641 acgaggtgac cgacatcgag cgcgaccgag agtccgagat cgacgatcac cggctcgatg
     2701 agcgcgaagt cgtaatcgag aacttcgacg catagatcgc gcggtgcgat accggaggcg
     2761 aggatcgatt cgagcgacag ccgggcacgc gcgcgccacg catcgaggtc cgacggaatg
     2821 ttgggatcgt gttcgtgctc gccgaggtag acgtgaacga tcgcaacctc ggggcgaaac
     2881 gggatcgcca cctcgagcgc acgccgtacc gacgagaccg acgcttgccg ccgcgattcg
     2941 ttggcgctcg cgaggctcgc gtcgagcggc gtgtgcagcg agtaggtgat cccggtacgc
     3001 gccttgtgct ccgccaagtg gcgagcttca tcagcacccg gcagtccgtc gctttcgaag
     3061 aacaagatct cgacatcttc gacccgacca gcgagtcgtt cgacattgaa cgcccatcca
     3121 ccggggtgaa tgaacgatgt ggttcccaag cgaaatggca cgttcatcat ggcctgacga
     3181 aacgacgtgt tgacgaggtc atggtgtttc cgtatggtgc cgacggtttt gacaatcgaa
     3241 tagcgatcgc aggttcgcgc gacgaggttt gcgcgaaaaa gggaagccgg tcaaacaccg
     3301 gcgcggtccc gccactgtag gtggagagtc acttcgaatt accactggcc cacgggctgg
     3361 gaaggttgaa gcggcgttcc ggcattgccg gaagatccac gagccaggaa acctgcctgc
     3421 gatcgatgaa ccttctcgcg gagaacgagg acggacttcg gcattgatcg gccgcatccg
     3481 cctcagctcc ggcttcgagg cactatgcgg tggagatggt tcg
//
LOCUS       DASUFX010000371        16231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_199492,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000371 DASUFX010000000
VERSION     DASUFX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199492"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFV99_14815"
     CDS             complement(<1..207)
                     /locus_tag="VFV99_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060637.1"
                     /translation="MSEKSVSRGFRGRRQPGEVAGRLPPGQYVERGFPVLSAGPTPRT
                     PLDRWDFSVEGNSDQSRRWSWDELR"
     gene            425..1825
                     /gene="zwf"
                     /locus_tag="VFV99_14820"
     CDS             425..1825
                     /gene="zwf"
                     /locus_tag="VFV99_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5060638.1"
                     /translation="MTTEPMSDALVIFGATGDLAYKQIFPALQAMVRRGALTVPVIGV
                     ARTRRSDDDLRERARDSVEHHGGFDREAFDKLARLLRYVSINYSDPQSFADLNRALDG
                     AKRPLHYLAIPPSAFTTAVAGLTRVRGTDNARVALEKPFGRDRASARALDKCLHDVFP
                     ESAIFRIDHYLGKEPVQNLLYFRFANSFPEPLWNRELVSSVEITMAEDFGVAGRGSFY
                     EETGAIRDVLQNHLLVVTSILAMDGPVGQNVEAVRDEKARVLKAIAPLDRSRVVRGQY
                     RGYRDERGVAPGSMVETFAAVELQIDTWRWAGVPFFMRTGKLMPCTATEVMIQLKKPP
                     REIFGEHISCPDYFRFRLEPEIVIALGIRVRRPGAATSDSMVGEDKELLASEDPKLGT
                     LPYERLLGDAMRGDQSLFARQDAIEEQWRIVDPVLGDAVPLHYYDKGTWGPAEAERVS
                     PWGCSNPLAPARRKSS"
     gene            complement(1860..2786)
                     /locus_tag="VFV99_14825"
     CDS             complement(1860..2786)
                     /locus_tag="VFV99_14825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5060639.1"
                     /translation="MKAIVVTDQAAGTAGMKLMERPEPQVSGPNYGDVVVQVRASGFT
                     GNELSWPSTWIDRLGRDRTPSIPGHELAGVVTALAYGTTGLTVGQRVFGLTDWTRDGT
                     LAEYIAVEARNLAPLPGDVDFTVGAGLVMSGLTAWQGLFEHGRLRAGSSVLVHGAAGV
                     VGSMATQLAREAGAYVIGTGRASARQTALDFGAQEFVDLDNDALEDVDGVDLVFDVLG
                     GDIAKRSAGVIRAGGTLVTITGPTEARPKSGLTIDFVVVPDRAQLSLLVQRVRDGRLR
                     TNIGNVAALDDAVAAFNPTERIKGKTIIRVHP"
     gene            2978..4000
                     /locus_tag="VFV99_14830"
     CDS             2978..4000
                     /locus_tag="VFV99_14830"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5060640.1"
                     /translation="MKRYLFVALLAACPKSGDKPKPVEPAGRGDAQSRLLPEMDAGPL
                     TLAPAPPLPAVPAGLPTPPAHPRVTPEALALGELLFYDTRLSQSGSRSCANCHDPATG
                     YSGNVQLAADGKQNLRRTPALVNLAWAKEFGWDGRYGTFAEHLPPHLKGQLGDPLDAV
                     TARIDAVPLYHLHLARIGGTPQDAIAQALEAFVLTRYEGDTPWDRMERTALTKAGTAS
                     SDPIVAGYQLFVGKAQCGQCHPPPLYTDHGFHKVAPNPFGDKGKAGGFKTPTLRGAAN
                     RTSFFHAGSVKTLEDAVAQYQKPPADVDPVLAKIKLTPDEAAQLVAFLKALTADRPAP
                     TKPVLP"
     gene            4029..4496
                     /locus_tag="VFV99_14835"
     CDS             4029..4496
                     /locus_tag="VFV99_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060641.1"
                     /translation="MWGRRLRYVLTLLALCSIATCPAAKRACTAKNRSREAENLLGVL
                     ADRVATVVATTGKVPPTPAGPTPQPSCCEQGGECKADPSMWATPGWRELQFSVDGAHR
                     YTYQYVPDASGQSAVLRATGDLDCDDVLGVYELRVTVKGTSLLRAWHRQNPYE"
     gene            4509..4931
                     /locus_tag="VFV99_14840"
     CDS             4509..4931
                     /locus_tag="VFV99_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5060642.1"
                     /translation="MRMLIALLLCVACSKNNEPPPPSSGSPMAQPESGPKGKKPAASA
                     GIPEKSQQMFMTVCATCHGADGSGNGPAAETLTTKPRNYTDPAWQASVTDDVIRNTIL
                     KGGQAMGKSPLMPGNPQLAEDPETLDGLVQIIRGFGKK"
     gene            4928..6511
                     /locus_tag="VFV99_14845"
     CDS             4928..6511
                     /locus_tag="VFV99_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060643.1"
                     /translation="MTDAAPLTLVTSHDGQWAVARQDRKLVLMPGAGGAPVASVELSS
                     ADVDIALVGPPTALIVLSRDPDARGIVLYTTPYLEAAARIDLDIDARLAAASGPRVAV
                     ASADNKNVAVVRSAGRALATQKLDLTPPIEFIVGLERNQMLFGLSKKLEVWDAVSGRP
                     MLRAQFQLPPPPRVLGASAGHLWARQIGSDEIFLYRLSDGRPFRHDAGAPISDVICHP
                     ASPLLVLVTPKGLLRLHGYAHSLTPLDDVPDNHGALALHVSGEDITLLGFTGVGADMW
                     RLPISGSSTGAIAVQTATPSAVPVASETARPSTPATASRWREPLATYGAELARGSEAE
                     LPIVAIDNELGDLAHRLELSATARRVLTALYALHLIGESISIAALAKATGDWTEALGQ
                     GELGALAMLRRKHGKIGLRTAVRDFLDGAAPRSIRIVGTGATTPRTGAFRVSRDSRTD
                     AEIETALAGELGRIAVVDGSLRSALLEARLHGATAIAYSVPAERPRPWPRDAGLVLVL
                     YGTNASWVADLPTLGATAS"
     gene            complement(6498..7346)
                     /locus_tag="VFV99_14850"
     CDS             complement(6498..7346)
                     /locus_tag="VFV99_14850"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5060644.1"
                     /translation="MSREAELYKDKIEVSLDGRQIFYLFFGGAVIVGLVFVLGVMVGR
                     RVEARGHTDRARVAATVDPLAALDRLEGGNGLSFQGALRGADGPVSDVEKTIAAMPQK
                     GSRPQASGFSQKDDPKSKKDEPKVDVKKDEPKAEAKKDEPKAEVKKDEPKKAEAKKDE
                     PKAEMKKDEAKKVDVKKDEAKVEAKKDEAKKDEAKKDEAKKDEPAKAKFTLQLSSFQD
                     KTEAQAYLDSMKNAGYSAYITEGAVDGKQYFRVRMGSYKSMEAANDAKAELEKSTKKN
                     AIVMKL"
     gene            complement(7430..10726)
                     /locus_tag="VFV99_14855"
     CDS             complement(7430..10726)
                     /locus_tag="VFV99_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060645.1"
                     /translation="MKVYRLAFVAVATLVACSDTTTNPVTQLNLDRPVDVAFACYGGL
                     RLTHGNAATGNEDLTASAQPVDACNIRSGPRPKAEPGQPTPPPPVPPGQEDIGTQKVG
                     SAAWYAFLLQSGPGTVAVARFPTQPSSLFAGGDVAVLDSDQLTPGKNGISVGEDPVAI
                     ATDKVGCYEVVANAGSCDLSSLDVGSAVQAASGDNVTPVVVRMDVKNSAGEVIRARPA
                     AMAFEPPGGTIGDECPAQPTGLAYIAYPGCRLIAGVDVATGTIVTGVQFDAAGVATVV
                     NGNVTCPDECSGAATMPGFRPVALDLEKDVRTGRTLLAIGADNSNALTVFDLDPVTSL
                     PLSMVPPILLENPSGKLGITSLAISPVIGMGGTSGSTSGVIDDTAPGGDHQFIYAVAT
                     DGSVRVADLSGAPRECDTNVDPRYLHNVRDADRLSCLGVGLATTPPRRAGVRGPGIEL
                     IGDAIPTSVDVTKVDRLDTATPPANPQQLIGYFGLITAANGQTFVFNVDNDDFADFVS
                     GGTLSEQLATPIPLDIAHQLRDGVPQRDAIADEGDPNNSMAPRHFVCDNPGPDPDAAG
                     GFSGGPRLTGSIQQSLPSGVLAAEKTGGLPSIRQVFCDSGGEDPDRPVSELYFSAPLD
                     VREDEFPDLRALRADETWTLTWEGALSGDKSDTAIDGPVVRQSQMFVDGAGMRLVDTT
                     RPFCDAGVEPYDVVQFRGCDPAVGDNGCPIGYTCYVHPQSQVTGLGSCMLEDEAERLS
                     NACKAFLTSLRRYTVGRTKSGELQLLPRKSVLRTTPIDGCTSDQQCEDLADYALRNTS
                     SANPSDPDQPTDPKTYRCQADPDRRPMPATSTGKRCLLTCNDDTQCALGTVCQAHPGA
                     APQMGYCMEGVTPPQACVNAPQRFELRAGEAFTVLGTRQGYMHPIIANPGDDVCVRDP
                     NANRMQIGRFPLDPPACDPTADPRTGALPGGGFEPNPCTLTTKETEFQLNYLDDTCTL
                     ADPDETIVTRDATAIRFRNRALNLTVVDPTYQGDLRCNGDRKGTLVNVPLVAPGYQIA
                     FRQVAGFVGLTIAGIAPALPIKVLRGPTQSFWIIDEGDFLSTSITQPSTRGKVFRVEA
                     SRLNAINLLE"
     gene            complement(10723..12051)
                     /locus_tag="VFV99_14860"
     CDS             complement(10723..12051)
                     /locus_tag="VFV99_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060646.1"
                     /translation="MRGLWLGILLVGCTASGKDVEPPDNELFFPTGAAVSPDEQFLFV
                     ANANSELRWDSGSISVIDLNAVDAAVADWIANQDDKILPDCAMDKDHRETLQCKEYNF
                     INVDASTRIGNFATDISVQDTGNGTLRLFVPTRGDPSIAWVDFDGSRLHCTDAQGWSQ
                     CDEAHRLSFVHNDPDLAPIPDEPFDAFADSKGQFAVVTHLTRGAVSLIDSPIGKPAVV
                     ADVLTELFASDPLSGSKAATGIAARPSTSDNGGIIYVASRSEDRIQTLTVGRPVNDAP
                     PYLIAGNYFFLNLVGTLAGSSTDSRGVTFSPNGDRMYLINRKPPSLQIYDTSVGPSGF
                     PKNAGIGATDICRQASTLAVMDTGDGERAYVTCFQDGQLYVVDPRGLSSVENIVTVGR
                     GPYSVVVAPNRKKVYVTNFLEDTVVVLDANPASPYYNRIVLRIGDVRPLK"
     gene            complement(12108..16019)
                     /locus_tag="VFV99_14865"
     CDS             complement(12108..16019)
                     /locus_tag="VFV99_14865"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX5060647.1"
                     /translation="MKWVRQFGGGSAEGRSKDKALLGGKGANLAEMASLGLPVPPGFT
                     ITTEVCRHVMEHGVGAYPAELDGEVGAALGRVEELTKTKFGDDQHPLLLSVRSGAPAS
                     MPGMMDTILNLGLNDTTVQALAVRTQNPRFAWDCYRRFVAMYGEVVMGVVPPTEHDEP
                     PFDKILDAIKAKHRANRDQDLSEAALREAVHAFKATAQEVTGIPFPDDVRKQLWGAIG
                     AVFASWNNPRADVYRKMNNIPASMGTAVNVQAMVFGNIGDDCATGVAFTRNPATGTPE
                     LYGEFLTNAQGEDVVAGIRTPEQIGELAKKLPAAHAELVRVAKLLEDHFRDMQDLEFT
                     IQKGQLFMLQTRNGKRTGKAMVKVAVDLVAEGKLTPKEAVLRIEPAKLDEVLHPTIDP
                     KSRPTPIAKGLPASPGAAIGRVVFTANAADEWHKRGEEVVLVRTETSPEDIHGMKAAE
                     GTLTARGGMTCIAGETKVLTDQGLLAAEAVFGKLEAGERLRILSFDSKSMKPVWRDII
                     AAGCKPAQVITVAVSQTGRVDDNVLRITDDHKTYYLYNRKLAKKPIKDVLAAEDFVLA
                     VDQVPGLGDTATTAALAYVAGAILSDGYIKVAPTKGSVTFIQKHTPEKVEFVAAVEQA
                     FLEAFEVPFSYGRNRETVATLRGREIRGEVQDRICFRKQPAERLAWIRDNLASWVLTL
                     DQTALRFFLAGYVDGDGSYAEESSAVRLEITVSQEKPGNLAGVVLACLRLGIVPQVTR
                     NRDAYNVQIAERIDEILAFTHRVLADAPDRKYESKCFSVRGLFDDIVDQVNFMGRVRE
                     GIKRNLTFGAEKIRRDVLPLCSGEQARELAAVLDSGVRSYRAKQVGDHEPATVYNFEV
                     DASDELDKNFVVFSSRLTPILVSNSHAAVVARGMGKPCITACTSLRVDAAKKTASFVG
                     GGKEFKIKEGDVLTLDGSTGEVFLGKAPLVPAQLGGELEQLMQWVDQHRKLKVRTNAD
                     TPIDAKTARAFGAEGIGLCRTEHMFFQPERILAVREMILASDEPGRRAALAKILPMQR
                     SDFAELFRVMDGLPVTIRLLDPPLHEFLPHTEAEIEEVAKDLGKPAGVIAAKVSELTE
                     ANPMLGHRGCRLAITYPEIYEIQVQAIGEAAALVARDGVAVHPEIMIPLVMVSEELRR
                     LRNLVANAMDKALGKAEIPYTIGTMIELPRACVVADRIAEHADFFSFGTNDLTQTTFG
                     LSRDDAGRFLPTYVDGGVLPNDPFAVLDPDGVGALIELGVRGGRGTKPGLKVGICGEH
                     GGEPSSVEFCHRAGFDYVSCSPFRVPIARVAAARAALAREK"
     gene            complement(16016..>16231)
                     /locus_tag="VFV99_14870"
     CDS             complement(16016..>16231)
                     /locus_tag="VFV99_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060648.1"
                     /translation="AERAARDKRDYAAVFESLEKLRPTVAAFFDKGGVMVMDPDPKLR
                     DNRLALLNWFVTPYMAIADFRLLAGAA"
BASE COUNT         2676 a         5465 c         5291 g         2799 t
ORIGIN      
        1 gcggagctcg tcccacgacc atcgcctgga ctgatcgctg ttgccttcga ccgagaaatc
       61 ccaccggtcg agtggcgtac gtggcgtcgg tcctgccgac agcacgggaa atccacgctc
      121 gacgtactgc cccggcggta ggcgcccggc gacttcgccg ggctgccggc gaccacgaaa
      181 tccgcgcgac acagattttt cactcatgac ctgacgctag gtgctgcacg cgccggacca
      241 tcgcgagcga tcagcgcgct gtgcctttca cttgcgaaca ccgtggcgca tcccggcacg
      301 ccactcgcac gtatcgatga cgtgcccgag gagaaacacg gagaacacag atgagaatcg
      361 gcatgattgg actcggccgc atgggcgcga acttcgaatt tggcggtcac gaggagaagc
      421 ggccatgacc acggagccca tgtcagacgc gctggtgatc ttcggtgcca ctggcgacct
      481 cgcgtacaag cagatcttcc cggcgctgca agcgatggtg cgtcgcggtg ccctgaccgt
      541 gccagtcatc ggtgtagcga gaacccgccg cagcgacgac gacttgcgcg aacgcgctcg
      601 cgacagtgtc gagcaccatg gcgggttcga tcgcgaagcg ttcgacaaac ttgcgcgctt
      661 gctgcgctac gtctcgatca actatagcga tccgcagagc ttcgccgact tgaatcgcgc
      721 actcgatggc gcgaagcgac cgctgcacta tctcgcgatt ccgccgagtg cgttcacgac
      781 cgcggtcgca gggcttaccc gagtgcgcgg cactgacaac gcgcgggtcg cgctcgagaa
      841 gccgttcggg cgcgatcgtg cttcggcgcg tgcgctcgac aagtgcctgc acgatgtctt
      901 cccagagtcg gccatctttc gtatcgacca ctacctcggc aaggagccgg tgcagaatct
      961 gttgtacttc cgcttcgcca actcgtttcc cgagccgctg tggaatcgcg agctcgtgag
     1021 cagcgtagag atcacgatgg ccgaagactt cggcgtcgct gggcgcggca gtttctacga
     1081 ggagaccggc gcgattcgcg acgtcctcca gaaccacctg ctcgtggtga cctcgatctt
     1141 ggcgatggac ggccccgttg ggcagaacgt cgaagcagtg cgcgacgaga aggcgcgcgt
     1201 gctcaaggcg atcgcgccgc tcgatcgctc ccgcgtcgtg cgcgggcagt accgcgggta
     1261 ccgcgacgag cgcggcgtcg cgccgggctc gatggtcgag accttcgcag ccgtcgagct
     1321 ccagatcgat acctggcggt gggccggcgt cccgttcttc atgcgtaccg gaaagctgat
     1381 gccgtgcacg gctaccgaag tgatgatcca gctcaagaag ccgccgcgcg agatcttcgg
     1441 cgaacacatt tcttgccccg actacttccg ctttcgcctc gagcccgaga tcgtgatcgc
     1501 gcttggcatc cgcgtcaggc gcccgggtgc cgcgacgagc gactcgatgg ttggcgaaga
     1561 caaggagctg ctcgcatctg aagacccgaa gctagggacg ttgccctacg agcgactgct
     1621 cggcgacgcg atgcgcggtg atcagagtct gttcgcccgc caggacgcga tcgaggagca
     1681 gtggcgcatc gtcgatccgg tgctcggcga cgccgtgccg ctccactatt acgacaaggg
     1741 cacctggggc cccgccgagg ccgagcgggt gtcgccctgg ggttgcagca accccctcgc
     1801 accagcccgg aggaaatcct cgtgactcga gcgtgcgata gaaggcaatc cccgaatcct
     1861 cacggatgaa cgcggatgat ggtctttccc ttgatccgct cggtcgggtt gaaggccgca
     1921 accgcatcgt caagggcagc cacgttgccg atgtttgtcc gcagccgacc atcccggact
     1981 cgctggacga gcagactcaa ctgggcgcga tcgggtacga cgacgaagtc gatcgtcagg
     2041 ccgctcttcg gccgcgcctc ggtcggaccg gtaatggtca ccagcgttcc tccggcgcga
     2101 atcacgcccg cagaccgctt cgcgatgtca ccgccgagga catcgaaaac cagatctacc
     2161 ccgtcgacat cttccagggc gtcgttatcg aggtcgacga actcctgtgc gccgaagtcg
     2221 agagccgtct gacgggcgga agcgcgcccg gtgccgatga cgtacgcgcc ggcctcacgt
     2281 gcgagctggg tcgccatcga accgactacg ccggccgcac cgtgcacgag gacgctcgac
     2341 cccgcacgaa ggcggccgtg ctcgaacagc ccctgccacg cggtcaggcc cgacatcacg
     2401 aggcccgcac ctacggtgaa gtcaacgtcg ccgggcagcg gtgcgaggtt gcgtgcctcg
     2461 acggctatgt actccgccaa cgtgccgtcg cgagtccagt ccgtgaggcc gaacacccgc
     2521 tgtcccaccg tcaaccccgt cgttccatag gcgagggcgg tgaccactcc ggccagctcg
     2581 tgacccggga tcgaaggcgt ccggtcacgg cctaggcggt cgatccaggt cgagggccac
     2641 gacagctcat tcccggtgaa tcccgacgca cggacctgaa caacgacatc gccgtagttc
     2701 gggcccgaca cctgcggctc gggccgctcc atcaacttca tcccggccgt tcccgcagcc
     2761 tgatcggtca ccacgatcgc cttcatcggt cacctcccta tgtcgagctc ttgccttgag
     2821 gctttgcact tttgattccg tagtcccacg cagcgcgtgc gctgcatgag ctgccgcccg
     2881 ccgaagctat gttgcgcagc actgataacc gtcacgcgtc tccgtttcgg gagtacggcg
     2941 ttgcccgcgt ggcggttcga cgcagtaagc tcgcggcgtg aagcggtacc tgttcgtcgc
     3001 gctgctcgct gcgtgtccga agagcggcga caagccgaag cccgtcgagc ctgcgggtcg
     3061 cggtgatgcg cagagccggc tgttgccgga gatggatgcc ggtccactga cgctcgcccc
     3121 tgccccgccg cttcccgcgg tgccggctgg cctaccgacg ccgcccgcgc atccgcgcgt
     3181 cacgcccgaa gcgctcgcgc tcggtgagct gttgttctat gacacgcggc tctcgcagag
     3241 tggctcgcgg tcgtgcgcga actgccacga tccggcgacc ggctattcgg gcaacgtgca
     3301 gctcgcggcc gacggcaaac agaacctgcg ccgcacacca gcgctcgtga atctcgcgtg
     3361 ggcgaaggaa ttcgggtggg acggccgtta cggaacgttc gccgagcacc tgccgccgca
     3421 tctgaaaggc cagctcggcg atccgctcga cgcggtaacg gcgcgcatcg atgcggtgcc
     3481 gctctatcac ctgcacctcg cacggatcgg cggcacgccg caggatgcga tcgcgcaagc
     3541 gctcgaagca ttcgtgctca cccgctacga gggcgacaca ccgtgggacc gcatggagcg
     3601 caccgcgctc acgaaagccg gtactgcctc gagcgatccg atcgtcgccg gctaccagct
     3661 gttcgtcggc aaggcgcaat gcggccagtg tcatccgccg ccgctctaca ccgaccacgg
     3721 ctttcacaag gtcgcgccga atccgttcgg tgacaaaggc aaggccggcg gattcaagac
     3781 gccgacgctc cgcggtgcgg cgaaccgcac gtcgttcttt catgccggca gcgtgaagac
     3841 gctcgaggac gcggtcgcgc agtatcaaaa gccgccggcg gacgtcgatc cggtgctcgc
     3901 caaaatcaag ctcacgcccg acgaggccgc gcagctcgtg gcatttctga aggcgctgac
     3961 ggcggatcgg cccgcaccga cgaaaccagt gttgccgtag cagtccttgc gctagattcc
     4021 acgggcccat gtggggccgc cgccttcgct acgtgctgac gctgctcgcg ctgtgctcga
     4081 tcgcgacgtg cccggccgcg aagcgcgcgt gtaccgcgaa gaaccgctcg cgcgaggccg
     4141 aaaacctgct cggtgtgctc gccgatcgcg tcgccacggt ggtcgcgacg accggcaagg
     4201 tcccgcccac gccggcagga ccgaccccgc agccgtcgtg ctgcgagcaa ggcggcgagt
     4261 gcaaggccga tcccagcatg tgggcaacgc ccggatggcg cgagctccag ttctccgtcg
     4321 atggcgcgca tcgctacacg tatcagtacg tgcccgatgc cagcggccag tcggccgtcc
     4381 tgcgcgctac cggcgacctc gactgtgacg acgtgctcgg cgtttacgag cttcgagtga
     4441 cggtcaaggg cacgagcttg ttgcgcgcct ggcatcgcca gaacccgtac gagtaagatg
     4501 tccgcatcat gcggatgttg atcgccctgc tcctctgcgt cgcctgcagc aaaaacaacg
     4561 agccacctcc accgagtagt ggctcgccga tggcgcagcc ggagtctggc cccaaaggca
     4621 agaagcccgc ggccagcgcc ggcattcccg agaagtcgca gcagatgttc atgaccgtgt
     4681 gcgcgacctg tcacggcgcc gacggcagcg gcaacggccc ggcggcggag acgctgacca
     4741 cgaagccgcg caactacacc gacccggcgt ggcaagcgag cgtcaccgac gacgtgattc
     4801 gcaacacgat cctcaagggc ggtcaggcga tgggcaagag tccgctcatg cccggtaacc
     4861 cgcagcttgc cgaggatccg gaaacactcg acggcctcgt gcagatcatc cgcggcttcg
     4921 gcaaaaaatg accgacgccg cgccgctcac gctggtgacg agccacgacg gccagtgggc
     4981 ggtcgcgcgt caggatcgca agctcgtgct gatgcccggt gcgggtggcg cgccggtcgc
     5041 gagcgtcgag ctgtcgtcgg cggatgtcga catcgcgctc gtcgggccac cgaccgcact
     5101 catcgtgctg tcgcgcgatc cggatgcgcg cggcatcgtg ctgtacacga cgccatatct
     5161 cgaagcggcg gcgcgcatcg atctcgatat cgatgcccgg ctcgcggcag cctcgggtcc
     5221 gcgcgtcgcg gtcgcatcgg ccgacaacaa gaacgtcgcg gtcgtgcgtt cggccgggcg
     5281 cgcgctcgcc acgcagaagc tcgatctcac gccaccgatc gagttcatcg tcgggctcga
     5341 gcgcaatcag atgctgttcg ggctctcgaa gaagctcgaa gtctgggacg cggtatcggg
     5401 gcgaccgatg ttgcgtgcgc agtttcagtt gccgccgcct ccgcgcgtgc tcggcgccag
     5461 cgccggtcac ttgtgggcgc ggcagatcgg cagcgacgag atctttctct accggctttc
     5521 cgacggccgg ccgtttcgtc acgacgccgg cgcgccgatc agcgacgtga tctgtcatcc
     5581 cgcatcgccg ctgctcgtgc tcgtcacccc gaaaggactg ctgcgcctgc acggctacgc
     5641 gcacagtctc accccgctcg acgacgtgcc ggacaatcat ggtgcgctcg ccctccacgt
     5701 cagcggcgag gacatcacgc tgctcggctt caccggcgtc ggcgccgaca tgtggcggct
     5761 gccgatcagt ggcagcagca cgggtgcgat cgcggtgcag accgcgacac cgagcgctgt
     5821 accggtcgca tcggagaccg cgcggccgag cacgccagcg actgcgagcc gctggcggga
     5881 accactcgcg acctatggcg cggagctcgc gcgcggtagc gaagccgagc tgccgatcgt
     5941 ggcgatcgac aacgagctcg gcgatctcgc gcaccgcttg gaactgtcgg cgactgcgcg
     6001 ccgcgtgctg accgcgctct acgcgctaca tcttatcggc gaatcgatct cgatcgccgc
     6061 actcgcaaag gcgaccggcg actggaccga agcactcggc caaggcgagc tcggtgccct
     6121 cgcgatgctg cgccgcaagc acggcaagat cgggctgcgc accgcggtcc gcgatttcct
     6181 cgacggcgcc gcgccgcgtt cgatccggat cgtcggcact ggcgcgacga cgccacgcac
     6241 cggcgcattt cgcgtgtcac gcgattcgcg cacggacgcc gagatcgaga ccgcgctggc
     6301 cggcgagctc ggccggatcg cagtcgtcga tggctccttg cggtcggcac tgctcgaagc
     6361 gcgactgcac ggcgcgacgg cgatcgcgta cagcgtgccc gccgaacggc cgcgcccgtg
     6421 gccacgcgat gctgggctgg tgctcgtgct ctacggcacg aacgcgtcgt gggttgcgga
     6481 cctgccgacg ctcggcgcta cagcttcatg acgatggcgt tcttcttcgt cgacttctcg
     6541 agctctgcct tcgcgtcgtt cgccgcttcc atcgacttgt aggagcccat gcgaacgcgg
     6601 aagtactgct tgccgtcgac ggcgccctcg gtgatgtacg ccgagtagcc cgcgttcttc
     6661 atcgaatcca gataggcctg cgcttccgtt ttgtcctgga acgacgacag ctgcagcgtg
     6721 aacttcgcct tcgcgggctc gtccttcttg gcctcgtcct tcttggcctc gtccttcttg
     6781 gcttcgtcct tcttggcctc gaccttcgct tcgtccttct tgacgtcaac cttcttggcc
     6841 tcgtccttct tcatctcggc cttcggctcg tccttcttcg cttcagcctt cttcggctcg
     6901 tccttcttca cctcggcctt cggttcgtcc ttcttcgctt cggctttcgg ctcgtccttc
     6961 ttcacgtcga ccttcggctc gtccttcttt gacttcggat cgtccttctg gctgaagccc
     7021 gaagcctgag gcctggagcc tttctgaggc atcgcggcga tcgtcttctc gacgtcgctc
     7081 accgggccgt ctgcgccgcg cagcgcgccc tgaaacgaca gcccgttacc gccctcgagg
     7141 cgatcgagtg ccgctagcgg atcgaccgtg gccgcgaccc gtgcgcggtc ggtgtgaccg
     7201 cgggcctcga cgcgccggcc caccatcacg cccagtacga acaccaggcc cacgatgacc
     7261 gccccgccga agaacaaata gaagatctga cgaccgtcga ggctgacctc gatcttgtcc
     7321 ttgtacagct cggcttcgcg cgacatagcc cgagggtcag cggtgccgat cccgggggca
     7381 agaattcaac cggtcggcgc ccacagagaa tgatccaccc aacggctgat cattcgagca
     7441 ggttgatagc gttgagccgc gacgcctcga cgcggaagac tttgccgcgc gtcgagggct
     7501 gggtgatcga cgtcgacagg aagtcgccct catcgatgat ccagaagctc tgcgtcggtc
     7561 cgcgtagcac cttgatcggc aacgcaggcg cgatgcccgc aatggtcagc ccgacaaagc
     7621 cggccacctg gcggaacgcg atctggtagc ccggcgccac cagcggcacg ttgacgagcg
     7681 tgcccttgcg gtcgccgttg cacctcaggt cgccctggta ggtcgggtcg accacggtca
     7741 gattcagcgc acggttgcgg aaccggatcg cggtggcatc gcgcgtcacg atcgtctcgt
     7801 ccggatcagc aagcgtgcac gtgtcatcca ggtagttgag ctggaactcc gtctccttcg
     7861 tcgtgagcgt gcacgggttc ggttcgaagc cgccccccgg cagcgcgccg gtacgcgggt
     7921 ccgcggtcgg atcgcacgcc ggcggatcga gcgggaaccg gccgatctgc atgcggttcg
     7981 cgttcgggtc gcggacgcaa acgtcgtcac ccgggttcgc gatgatcgga tgcatgtagc
     8041 cctgacgcgt gccgagcacc gtgaacgcct cgccggcgcg gagctcgaac cgttgcggag
     8101 cgttcacgca cgcctgcggc ggcgtcacgc cttccatgca gtagcccatc tgcggcgcgg
     8161 cacccggatg ggcctggcac accgtgccga gcgcgcactg cgtgtcgtcg ttgcaggtga
     8221 gcaagcagcg cttgcccgtg gaggtcgccg gcatcgggcg ccggtccgga tcggcctggc
     8281 agcggtaggt cttcggatcg gtcggttgat ccgggtcgct cggattcgcc gacgatgtgt
     8341 tgcgaagcgc atagtccgcg aggtcctcgc actgctgatc cgaggtgcag ccatcgatcg
     8401 gcgtcgtgcg cagcacggac ttgcgcggca ggagctggag ctcgccgctc ttcgtgcggc
     8461 cgaccgtgta gcggcggagc gacgtcagga acgccttgca cgcattcgac aggcgctcgg
     8521 cttcgtcctc gagcatgcag gaaccgaggc cggtgacctg gctctgcgga tgaacgtagc
     8581 aggtgtatcc gatcgggcag ccgttatcac cgaccgccgg atcgcaaccg cggaactgga
     8641 cgacgtcata cggctcgaca cctgcgtcgc agaacggccg cgtcgtgtcg acgaggcgca
     8701 tgccggcgcc atcaacgaac atctggcttt gacgaacgac ggggccatcg atcgcggtat
     8761 ccgacttgtc cccggagagc gcgccctccc acgtcaacgt ccatgtctcg tcggcgcgga
     8821 gcgcgcgcag gtcagggaac tcgtcctcgc gcacgtcgag cggagccgag aagtacagct
     8881 cgctgaccgg gcgatcggga tcttcgcccc ccgagtcgca gaagacctgg cgaatgctcg
     8941 gcagaccgcc agtcttctcg gcggcaagca cgcccgatgg tagcgattgc tggatactgc
     9001 ccgtgaggcg cggaccgccg ctgaagccgc cggcggcgtc gggatccggg ccggggttgt
     9061 cgcagacgaa gtgccttggt gccatcgaat tgttcgggtc gccttcgtcg gcgatcgcat
     9121 cgcgctgcgg cacgccgtcg cggagttggt gtgcgatgtc gagcggaatc ggcgtggcga
     9181 gttgctcgct caacgtgccg ccgctgacga aatccgcgaa gtcgtcattg tcgacgttga
     9241 acacgaacgt ctggccgttc gctgcggtga tgaggccgaa gtaaccgatc agctgctggg
     9301 gattcgccgg tggggtagcg gtgtcaagcc gatccacttt cgtgacgtcg accgacgtcg
     9361 gaatcgcatc gccgatcagc tcgataccgg gcccgcgcac cccggcacga cgcggcggcg
     9421 tcgtcgcgag gcccacgccg agacacgaca gtcgatccgc atcgcgcacg ttgtgcagat
     9481 agcgaggatc gacgttcgtg tcgcactcgc ggggcgcgcc gctgaggtcg gcaacgcgca
     9541 cgctgccgtc ggtcgcgacg gcgtagatga actggtggtc gccgcccggc gcagtgtcgt
     9601 cgatgacgcc cgacgtgctt ccgctggtgc cgcccatacc gatgaccggc gagatcgcca
     9661 gcgacgtgat gccgagcttg ccgctcggat tctccagcag gatcggcggc accatcgaca
     9721 atggcaacga cgtcaccgga tcgagatcga agacggtgag cgcgttcgag ttgtcggcgc
     9781 cgatcgcgag cagcgtgcgg ccggtgcgca catccttctc gagatcgagc gcgacagggc
     9841 gaaagccggg catcgtcgcg gcaccgctac actcgtcggg acacgtcacg ttgccgttca
     9901 ccacggtcgc gacgccagcc gcatcgaact gcacgccggt cacgatcgtg cccgtcgcga
     9961 catcgacgcc ggcgatgagg cggcacccgg gatacgcgat gtaggcgagg cccgtgggct
    10021 gcgccggaca ctcgtcacca atcgtgccgc cgggcggctc gaaggccatc gcggctggac
    10081 gcgcgcggat cacctcaccc gcggaattct tcacatccat gcggacaacg acgggcgtga
    10141 cgttgtcgcc actcgcggct tgcaccgcac tgccaacgtc gagcgacgac agatcgcaag
    10201 agccagcgtt cgcgacgact tcgtagcaac cgaccttgtc ggtcgcgatt gcaaccggat
    10261 cttcgccgac gctgatgccg ttcttgcccg gtgtcagctg atcggagtcg aggacggcaa
    10321 cgtcgccacc ggcgaacaac gacgacggtt gggttgggaa ccgcgcgacc gcaacggtgc
    10381 ccggacccga ctgcagcagg aatgcgtacc acgcggccga gccaaccttc tgcgtgccga
    10441 tgtcttcctg gcccggcggc accggaggcg gcggggtcgg ctggcccggc tcagccttcg
    10501 ggcgagggcc cgagcggatg ttgcacgcgt cgaccggctg cgcgctcgcc gtcaggtctt
    10561 cattgcctgt ggcagcgttg ccgtgggtga ggcgcaaccc gccgtaacac gcgaacgcca
    10621 cgtcgaccgg acgatcgaga ttgagctgcg tgaccggatt ggtcgtggtg tcgctacacg
    10681 cgacgagcgt ggcgacggcg acgaatgcga gacggtagac cttcatttca gtggcctcac
    10741 gtcaccgatg cgcaacacga tgcggttgta gtagggcgac gccgggtttg catcgagcac
    10801 gacgaccgtg tcctcgagga agttcgtgac atagaccttc ttgcgattgg gcgcgacgac
    10861 gaccgagtac ggtccgcgcc cgacagtgac gatgttctcg acggacgaca acccgcgcgg
    10921 atcgacgacg tacagctggc cgtcctggaa acaggtgacg tacgcgcgct cgccatcgcc
    10981 ggtatccatc accgcgagcg tcgaggcctg acgacagatg tcggtcgcgc cgatgccggc
    11041 gttcttcgga aagccactcg gccccaccga cgtgtcgtag atctgcaggc tcggtggctt
    11101 gcggttgatc aggtacatgc ggtcgccgtt cggcgagaac gtgacgccac gcgaatcggt
    11161 cgagcttccg gcaagggtgc caacgaggtt caagaagaag tagttgccgg cgatcaggta
    11221 cggcggcgcg tcgttgaccg ggcggccgac ggtgagcgtc tggatgcggt cttcgctgcg
    11281 actcgcgaca tagatgatgc cgccgttgtc gctcgtgctc ggccgcgcgg cgatgccagt
    11341 cgcagccttc gagcccgaca gcggatcgga agcaaacagc tcggtgagca cgtcggcgac
    11401 gaccgcgggc ttgccgatcg gcgaatcgat gagcgacacc gcgccgcgcg tcaggtgcgt
    11461 gacaacggcg aactggccct tcgagtccgc gaatgcatcg aacggctcgt cggggatcgg
    11521 cgcgagatcc ggatcgttgt gcacgaagct caggcgatga gcctcgtcgc actgcgacca
    11581 accttgcgcg tcggtgcagt gcagccgcga accatcgaaa tcgacccatg cgatcgacgg
    11641 gtcaccgcgc gtcggcacga acaggcgcag cgttccgttg cccgtgtcct gcaccgagat
    11701 gtcggtcgcg aagttgccga tgcgcgtgct cgcatcgacg ttgatgaagt tgtactcctt
    11761 gcactgcaac gtctcgcgat ggtctttgtc catcgcgcag tccgggagga tcttgtcgtc
    11821 ctggttcgcg atccaatccg cgacggcagc gtcgaccgcg ttgagatcga tcaccgagat
    11881 cgaaccggag tcccagcgca gctccgagtt cgcgttcgcg acgaacagga actgctcgtc
    11941 cggcgatacg gccgcgcccg tcgggaagaa cagctcgttg tccgggggct ccacatcctt
    12001 gccggatgcc gtgcaaccta cgagcaaaat gccgagccaa agccctcgca tgagatggcc
    12061 cttcgacctc cacatccgcg aaaccgttgc ctgatacatg acgtgaatca cttctctctc
    12121 gccagggcgg cgcgtgctgc cgcgacccgc gcaatcggca cccggaacgg ggagcacgat
    12181 acgtagtcga accccgcacg gtggcagaac tcgacggagc tgggctcacc gccatgctcg
    12241 ccacaaattc caacctttag gccgggtttg gtgccgcgtc cgccgcgcac gccgagctcg
    12301 atgagcgccc cgaccccgtc ggggtcgagg accgcgaacg ggtcgttcgg gagcaccccg
    12361 ccgtcgacgt acgtcggcaa gaaccggccg gcatcgtcac gggataggcc aaaggtggtc
    12421 tgggtcaggt cgttggtgcc gaacgagaag aagtcggcat gctcggcgat ccgatcggcc
    12481 accacgcacg cgcgcgggag ctcgatcatc gtgccgatcg tgtacgggat ctccgccttg
    12541 ccgagcgctt tgtccatcgc gttggcaacg aggttgcgca gccggcgcag ctcctcggac
    12601 accatcacga gcgggatcat gatctcggga tgcacggcga cgccgtcgcg cgcgaccagt
    12661 gccgcggctt cgccgatcgc ctgcacctgg atctcgtaga tctcgggata ggtgatcgcg
    12721 agccggcagc cgcgatggcc gagcatcgga ttcgcctcgg tgagctcgga gaccttcgcg
    12781 gcgatcacgc cggctggctt gccgagatcc ttcgcgacct cttcgatctc cgcctccgtg
    12841 tgcggcaaga actcgtgcag cggcggatcg aggagccgga tcgtgaccgg caagccatcc
    12901 atcacgcgga acagctccgc gaagtcgctg cgctgcatcg gcaggatctt cgcgagggcg
    12961 gcgcgccgtc cgggctcgtc gctcgcgagg atcatctcgc gcaccgcaag gatgcgctcg
    13021 ggctggaaga acatgtgctc ggtgcgacac agcccgatgc cctcggcacc gaaagcgcgc
    13081 gccgtcttcg catcgatcgg cgtgtctgcg ttcgtgcgga ccttcagctt gcgatgctga
    13141 tcgacccact gcatcagctg ctccagctcg ccaccgagct gcgccggcac gaggggcgct
    13201 ttgcccagga atacttcgcc cgtcgagcca tcgagggtga gcacgtcacc ctctttgatc
    13261 ttgaattcct tgccgccgcc aacgaacgaa gccgttttct tggcggcgtc cactcgcaac
    13321 gacgtgcacg cggtgatgca cggcttgccc atgccgcgcg ctactacagc ggcgtggctg
    13381 ttgctcacga ggatcggcgt cagccggctc gagaatacga cgaagttctt gtcgagctcg
    13441 tcagaggcgt cgacctcgaa gttgtagacc gttgccggct cgtgatcgcc gacttgcttt
    13501 gcgcggtacg agcgcacgcc cgaatcgagc acggccgcta actcgcgcgc ctgctcgccg
    13561 ctgcacaacg gcagcacgtc acgccggatc ttctcggcac cgaacgtcag gttgcgcttg
    13621 atgccttcgc gcacgcgtcc catgaagttc acctgatcga cgatgtcgtc gaacagaccg
    13681 cgcacgctga agcacttcga ctcgtacttg cggtctggag cgtcggcgag cacgcgatgc
    13741 gtgaatgcaa ggatctcgtc gatgcgctcc gcgatctgca cgttgtacgc gtcgcggttg
    13801 cgcgtgactt gcgggacgat gccgaggcgc aagcatgcca gcacgacgcc ggcgaggttg
    13861 ccgggcttct cctgcgagac ggtgatctcg agcctgaccg cgctcgactc ttcagcgtac
    13921 gagccgtcgc cgtcgacgta gcctgcgagg aagaaccgca gtgcggtctg atcgagcgtg
    13981 agaacccacg acgcgaggtt gtcgcggatc caggcgagac gctcggccgg ctgtttgcgg
    14041 aagcaaatcc ggtcttgcac ctcgccgcgg atctcgcgac cgcgcagcgt ggcgaccgtc
    14101 tcacggttgc gaccgtacga gaacggcacc tcgaatgcct cgaggaacgc ctgctcgacc
    14161 gcggcgacga attcgacctt ctccggggtg tgtttctgga tgaacgtcac cgagcccttg
    14221 gtcggtgcga ccttgatgta accgtccgac aggatcgcgc ccgcgacata cgcgagcgca
    14281 gctgtggtcg ctgtgtctcc gagtcccgga acctgatcga cggcgagcac gaaatcctcg
    14341 gccgcgagaa cgtccttgat cggtttcttc gcgagcttgc ggttgtacag gtagtacgtc
    14401 ttgtggtcgt cggtgatgcg aagcacgttg tcgtcgacgc gaccggtctg cgataccgca
    14461 acagtgatga cctgcgccgg cttgcacccg gctgcgatga tgtcgcgcca caccggcttc
    14521 atcgacttgc tgtcgaacga caaaatgcgc aggcgctcgc cggcttcgag cttgccgaac
    14581 accgcttcgg ccgcgagtag cccctgatcg gtgagcacct tcgtctcgcc cgcgatgcag
    14641 gtcattcccc cgcgcgcagt gagcgtgccc tcggcggcct tcatgccgtg aatgtcttcc
    14701 ggcgacgtct cggtgcgcac gagcacgact tcttcgccgc gcttgtgcca ctcgtcggcg
    14761 gcgttcgcgg tgaacacgac gcgaccgatc gcggcgcccg gcgacgcggg caatcccttc
    14821 gcgatcggcg tcggccgcga cttcggatcg atcgtcgggt gcagcacctc gtcgagcttc
    14881 gccggttcga tgcgcagcac tgcttccttc ggcgtcagct tgccttcggc gacgaggtcg
    14941 acggcaacct tcaccatcgc cttgccggtg cgcttgccgt tgcgggtctg cagcatgaac
    15001 agctgaccct tctggatcgt gaactcgagg tcctgcatgt cgcggaagtg atcctcgagg
    15061 agcttcgcga cgcgaacgag ctcggcatgc gcggccggca gcttcttcgc gagctcgccg
    15121 atctgctcgg gcgtgcgaat gccggcgacg acgtcttcgc cctgcgcgtt cgtgaggaac
    15181 tcgccgtaga gctcgggcgt gccggtggcc gggttacgcg tgaacgcgac accggtcgca
    15241 caatcgtcgc cgatgttgcc gaacaccatc gcctggacgt tgacggcagt gcccatcgac
    15301 gccgggatat tgttcatctt gcgatagacg tcggcgcgcg ggttgttcca cgacgcgaac
    15361 acggcaccga tcgcacccca gagctgcttg cgcacgtcgt cggggaacgg gatgccggtg
    15421 acttcttgcg ccgtcgcctt gaacgcgtgc acggcctcgc gcaacgcggc ctcgctgaga
    15481 tcttgatcgc gattcgcgcg atgcttcgcc ttgatcgcgt cgagaatctt gtcgaatggc
    15541 ggctcgtcgt gttcggtcgg cggcacgacg cccatcacga cctcgccgta catcgcgacg
    15601 aagcggcgat agcagtccca cgcaaagcgc gggttctgcg tgcgcacggc gagcgcttgc
    15661 acggtcgtgt cgttgagccc gaggttcagg atcgtgtcca tcatgccggg catcgacgcc
    15721 ggcgcgcccg agcgcaccga cagcagcagc ggatgctggt cgtcgccgaa ctttgtcttc
    15781 gtcagctctt cgacgcggcc gagcgcagcg ccgacctcac cgtcgagctc cgcgggatac
    15841 gcgcccacgc catgctccat cacgtgacga cagacttccg tcgtgatcgt gaagccgggt
    15901 ggcacgggca ggccaagcga tgccatctcg gcgaggttcg cgcccttgcc accgaggagt
    15961 gctttatctt tcgagcgacc ttcggcggag ccgccgccga attgacgtac ccatttcatg
    16021 cggcacctgc gagcaagcgg aagtcggcga tcgccatgta cggcgtgacg aaccagttga
    16081 gcagcgcgag ccggttgtcg cgaagcttcg gatcgggatc catgaccatg acgccgccct
    16141 tgtcgaagaa cgccgcgacc gtcggccgca gcttttcgag cgattcgaac accgcggcgt
    16201 agtcacgctt gtcacgcgcg gcgcgctcgg c
//
LOCUS       DASUFX010000372         9446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1589415, whole genome shotgun sequence.
ACCESSION   DASUFX010000372 DASUFX010000000
VERSION     DASUFX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589415"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFV99_14875"
     CDS             <1..801
                     /locus_tag="VFV99_14875"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX5060649.1"
                     /translation="DALRASLAHLHETGDDGVDAVTAIGAAPVFVAAWNRKRAGAAPV
                     APNAVLGHAADYLRMSLGVTPTDEAVRALDAYLVTVIDHGMNASTFTARVVTSTGSDL
                     VSAVVAAVGALKGPLHGGAPGPVLDMLDAIGEPARAEQWLLGELAAGRRIMGMGHRIY
                     RVRDPRAAVLEQAVKSLASQRLDLAHAVERAAAKILQERRPDRPLAANVEFYTAVLLD
                     AVGLPREQFSSTFAVGRIAGWSAHVIEQRRTGRLIRPNSKYVGPMPMY"
     gene            989..2809
                     /locus_tag="VFV99_14880"
     CDS             989..2809
                     /locus_tag="VFV99_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5060650.1"
                     /translation="MRGLVSLIAPADQPLELQILGRTLLHAALVGFGAGVLGCAFFAG
                     AELLQNVLLEQLAGYEPLRAHGERVWGGAASSHFRWWLLAIIPALGALVGSLFARLAP
                     ECRGGGGDATIEAFHHHGGAVRRRVLWVKPLASIATLGAGGSGGREGPTMQIGAALGS
                     TVGRHLRVTPRERRVLMVAGVAAGISAVFRAPLGAALIAIEMLYRDDFESEALIPSVL
                     ASVIAYSVSISVFGQTTLFGHLEPFAFKPAHIPLYIGLAIVVSVGASLFVGTLRLAQR
                     LTARMPIVDWARPAIGGLLLGVFVVLFLHFIGPFVDHGDRGLGILGGGYGASQVAITG
                     ADWLPLGWRGVQILVLLAAIKIVASSLTIGTGGSAGDFAPALAIGALLGGAFGLAARL
                     LFDDPTIHPGAFALVGMGTFYGGIANTPLAALVLVCEMAGSYELLVPLMLAGGVAFVA
                     LRRVTLYTAQVPTLRDSPAHRRDVDPLSRLRCADVLRRDRAFVRFAPQTRIPALTKQV
                     EAAADQDVFPIVDSAGALQGVIAAEALRVIASNPELHQVGVAADLMMPAVSIPLEADL
                     RSAAQLMVARDLRSVPVIDADGSIVGILDEHDIASATLDT"
     gene            complement(2816..4696)
                     /locus_tag="VFV99_14885"
     CDS             complement(2816..4696)
                     /locus_tag="VFV99_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060651.1"
                     /translation="MSRHQVRLLLCSIASVALIAGALFALEWFRVDFGPAQITIDLRE
                     MHMCAPSEACKTIDLASMQGLYPVSAIAAFWGALALGLLVIGQCFARVLAGSASVSAS
                     RLGYVLASAVFLCAFGAGYLFAPETDGMLGMMGATVNQTFAPPMLLAGCLFALLALRY
                     AQLDAVVDEAGEYKPVVLKKDLDARLPVTPIQTKQIEPAPRERRPTPASFERIPLPGR
                     TPTGERVASGRTPTGERVAPATTRDRVTDTPPLASKRPGTDSERTKAPSQQPAARERA
                     KTASDSPTGTGTSVGAPRTKTASQQQPMIARTVSPNEPVIARTKSPSREPDDGPTEVD
                     PMTRARTSSSGPIDLAARLSGAPIAVAIKPPLPEPVPVPPDQIPVAPESGLVIRKRTP
                     SSGPLTAKAAQAAAALVPAAPRTKTPTGTKPLSAIGRRITDDFDVATPIPAAMPMHEE
                     DMTGLGLSFEAEPARVSSVAATGSSPPGKLQPVSRDSAAPPGLRGKLNYAVTSATLSS
                     TGIEAMREDGSTRQVTWEAIVGIIARRLPNEAPFDGSTFVDLVSTAGSTLRILEWSQL
                     AGAPIHGSGEERARAFVQLVAARCLDAKLDSWTKVFADGVGRAAQLPSAKTLSAHDDR
                     LA"
     gene            complement(4781..7387)
                     /locus_tag="VFV99_14890"
     CDS             complement(4781..7387)
                     /locus_tag="VFV99_14890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5060652.1"
                     /translation="MAIAAIGLFLYMRRHRDERAPAPAVATKGSAQHAQHAAPEPADP
                     AKLVVAVTDDKGPLAGATVRFERERGDVDIVETGKDGVARADRLEPGTWQVSASADGH
                     EPAALRPRELRAGEVVNVDLKLAPGGRTLTGTVTDASGGPITGARIDAAKLGAVARPS
                     DAVASTLTGADGKYKLSVAEGQLLVAASESSYAPQSRFVEVGASGASADFQLVPGGVI
                     EGVVRDEQTREPVPGATVLAQRDAPAMMLGERATHDVKSGADGRFRITGLRPGGYELN
                     ARAVGRASQAPTIVGLGVAEQVTDVEIVVGRAPVIRGVVVDESGAPAPNVEVNAFSGP
                     GDNADAKADAKGQFALEGLAPGKYTLMARSEEFLPAGTTNVELEHKDLDGVKITVRRG
                     LKIKGHVERRQVCEVQLDFDEGAMQRGDMPMLIAPITTGPDGEFEVGPAHPIAYDLKA
                     RCPSGEQGTKKIDVKPGMPDVVIEVKPGASIAGKVLDGGGKPIGGVTVMAAATGGTER
                     TMIVNGMVTSGVQSLTNATGAFELRGLAAGTYRLRVLDRGRPLPMKPDAKIAVTATEH
                     KTGVTLTVDRPDGVIRGVVTGSDGKPIADAWVSVNQNLEDMLSEALANHGPDEGGSRS
                     VMIEAHDDGGGDTGGMPPALTDANGKFEIRNLARVPWTVVAEAQAGKLRGRATKVVPD
                     AQITLQAVGLTKLEGKLVTGGAAMPSSFQVELEGPTRAQRSFTTADGTFSFARVDPGE
                     YKVTVTSSAGNGSATVKVQPDQTAHVDINLAANAIVVGKLIDAAGKPVGGVPVTIIPD
                     TGDGQLRVELHGMPPTSNPDGTFRLDAKAGPSILMVMTPPRPTSKRGLNLEAGKTLDV
                     GPITVAATPAPP"
     gene            7538..8200
                     /locus_tag="VFV99_14895"
     CDS             7538..8200
                     /locus_tag="VFV99_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060653.1"
                     /translation="MHNSYEVGEDPTIGVAIAQPTQEKAVLIVTRPDGSTVRQRVSLG
                     VARNNIRFGGTVVDKSTEVTFTERGDYRVELRNDNALLAKQEIRISVDQLTQLFDDSE
                     IADFAPVVRYTRSRGLNKQQWKTYGALYEHTLRKGVQIQVLIEDPGDALDEAWKQYKE
                     EGTLGVIENNYVRFRDRNGSVSASWISGKKIVAMRAETLDDFERGFIGAFLARFPSKL
                     RH"
     gene            complement(8197..9087)
                     /locus_tag="VFV99_14900"
     CDS             complement(8197..9087)
                     /locus_tag="VFV99_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060654.1"
                     /translation="MRLYAALATLVVLVPIASAERRRHVRPHVEAARVHTEPPMRIRA
                     LAPPPVAPPPRPASLTLRSNAIQASVTAEMGMTSGAPVAAPASIAPDLSFGVTDAFTL
                     SANTSGSALTGFRGGAGAGICMGGVDRSCRVPFNGGGIEGLVSIATGSAAFALNAGVM
                     WSAIEPSVHTDLKLGFKLKMSEGKLFALVSPSVWLALDDRYDRVVPHEDQLFVPIGLF
                     VKPVAPLALGIGTGVKGPVKHFADRMAIPLGASATYAVDKHWTLGTSFVFGKLFAGSD
                     VMDPGIDARAIQVWVSVLSG"
     gene            complement(9198..>9446)
                     /locus_tag="VFV99_14905"
     CDS             complement(9198..>9446)
                     /locus_tag="VFV99_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060655.1"
                     /translation="IENCTLDRNYQGTCENPTAQQVAIDKLALAHRNEIAAKIVLTGS
                     VLAIVGAAAVWYTAPKERVVITPTATTSSVGVSLAGHF"
BASE COUNT         1465 a         3135 c         3182 g         1664 t
ORIGIN      
        1 gatgcgctgc gcgcctcgct cgcacacctg cacgagacag gtgacgacgg cgtcgatgcg
       61 gtgaccgcga tcggtgcggc gccggtgttt gttgccgcgt ggaatcgcaa acgcgcgggt
      121 gctgcgccgg tcgcgccgaa cgcggtgctc ggccacgcag cggattacct gcggatgtca
      181 ctcggcgtga caccgacgga cgaagcggtg cgcgcgctcg acgcgtatct cgtcaccgtg
      241 atcgatcacg ggatgaacgc gtcgacgttc accgcgcgtg tcgtcacatc gaccggatcg
      301 gatctggtct cggccgtggt cgctgcggtc ggtgcgctca aaggcccgct gcacggcggt
      361 gcgcctggcc ccgtgctcga catgctcgac gcgatcggtg aaccggcgcg cgcggagcag
      421 tggctgctcg gcgagctcgc agcaggccgc cgcatcatgg gcatgggcca tcgcatctat
      481 cgagtacgcg atccgcgcgc cgcggtcctc gagcaagcgg tgaagtcgct cgcgtcgcaa
      541 cgcctcgatc tcgcgcatgc ggtcgaacgc gcggctgcta agatcctgca ggaacgcagg
      601 ccagatcggc cgctcgccgc gaacgtcgag ttctacacgg cggtgctgct cgatgcggtc
      661 ggtctgcccc gcgagcagtt ctcgtccacg tttgcggtcg gccgcattgc cggctggagt
      721 gcgcacgtca tcgagcagag gcggaccggc cgcttgatcc gacctaattc gaagtacgtc
      781 ggcccgatgc cgatgtacta actatccggc atcttgcggg aacgctttgt actcaaagta
      841 gtttctcggt agtgctccag cacgaccggt gatctatgct gacgcggtgg ctaagggtcc
      901 ggaagatcga ggtcctgaac cgaatgaacg cgccagtgac agcggaaccg gaccgatcgc
      961 attcgcgcgc gccgccgtgc tgcggcccat gcgcggattg gtctcgctga tcgcaccagc
     1021 agatcagccg ctcgagcttc agattctcgg ccgtacgctc ctgcatgcgg cgctggtggg
     1081 cttcggagcg ggtgtgctgg gctgcgcatt tttcgcggga gccgagctgt tgcagaacgt
     1141 gcttctcgag cagctcgcgg ggtacgaacc gttgcgcgcg cacggcgaac gagtgtgggg
     1201 aggggcagcc tcgtcgcact tccggtggtg gcttctcgcg atcatcccgg cgctgggtgc
     1261 gctcgtcggc tcgctattcg cgcggctcgc gcccgagtgc cgcggtggtg gcggtgatgc
     1321 gacgatcgaa gcgttccatc atcacggtgg tgcggtccgg cggcgcgtgc tctgggtgaa
     1381 acccctcgcc tcgattgcga cgctcggcgc cggtggctcg ggcggtcgcg aagggccgac
     1441 gatgcagatc ggcgctgcac tgggctcgac ggtcgggcgc cacctgcgcg tgacgccgcg
     1501 cgaacgccgc gtgctcatgg ttgccggcgt cgcggccgga atctcggcgg tatttcgcgc
     1561 accgctcggc gccgcgttga tcgcgatcga aatgctgtac cgcgacgact tcgagtcgga
     1621 ggcgctcatt ccgagcgtcc tcgcatccgt gatcgcgtac tcggtctcga tctcggtctt
     1681 cggtcaaacg acgctgttcg gacatctcga accgttcgcg ttcaagcccg cgcacatccc
     1741 gctctacatc ggtctcgcga ttgtcgtatc ggtcggtgcg tcgttgttcg tgggcacctt
     1801 gcggctcgcg cagcggctga ccgcgcgcat gccgatcgtc gactgggcac ggccggcgat
     1861 cggcggcctc ctgctcggcg tattcgtcgt gttgttcctg cacttcatcg ggccgttcgt
     1921 cgaccatggg gatcgtggtc tcggcatcct gggtggcggc tacggcgcat cgcaggtcgc
     1981 gatcaccggc gccgactggc tgccactcgg ttggcgcggc gtgcagatcc tggtgctgct
     2041 cgcggccatc aagatcgtcg cgtcgagcct gacgatcggc accggcggaa gcgcaggtga
     2101 tttcgcgcct gcactcgcga tcggtgcgct gcttggcggc gcgttcgggc ttgcagcccg
     2161 gctgttgttc gacgatccga cgattcaccc gggcgcattc gcgctcgtcg ggatggggac
     2221 attctacggt ggcatcgcga acacgccgct tgccgcgctc gtgctcgtgt gcgagatggc
     2281 aggcagctac gagctgctgg ttccgctcat gcttgccggc ggcgtcgcgt tcgtcgcgct
     2341 gcgccgcgtg acgctctaca cggcgcaggt tccgaccctg cgtgactcac ccgcgcaccg
     2401 acgcgatgta gatccgctga gccgtctccg ctgtgcagac gtcctccgcc gcgaccgcgc
     2461 gttcgtgcgg ttcgcaccgc agacaagaat ccccgctctc acgaagcagg tcgaggccgc
     2521 ggccgatcaa gacgtgtttc caatcgtcga cagcgcgggc gcgttgcagg gcgtgattgc
     2581 cgccgaggcg ctccgcgtga ttgcctcgaa cccggagctc catcaggtcg gagttgcagc
     2641 cgatctgatg atgcccgcgg tctcgattcc gctggaagcc gatctcagga gcgcggcgca
     2701 actcatggtc gcgcgcgatt tgcggtcggt gccagtcatc gacgcggatg gcagcatcgt
     2761 cggcatcctc gacgagcacg acatcgcgtc ggccacgctc gacacgtaag tgcagctacg
     2821 cgaggcggtc gtcgtgagcg gagagcgtct tcgcgctcgg caattgcgcg gcgcggccga
     2881 caccatcggc gaacaccttc gtccagctgt cgagcttggc gtcgaggcag cgcgcggcga
     2941 cgagctgcac gaacgcgcgc gcacgctctt caccgctacc gtgaatcggg gcgccggcga
     3001 gctgcgacca ttccaggatg cgcaaggtcg agccggcggt cgacacgaga tcgacgaacg
     3061 tcgagccgtc gaacggcgct tcgttcggca accggcgcgc gatgatgccg acgattgctt
     3121 cccacgtgac ctgcctcgtc gagccgtcct cgcgcatcgc ctcgatgccc gtgctggaaa
     3181 gcgtcgcgct cgtcaccgcg tagttcagct tgccgcgcag ccccggcggg gcggcggaat
     3241 cacgcgatac cggttgcagc tttcctggcg gagacgagcc ggtcgcagcg accgacgaca
     3301 cgcgcgcggg ctcggcctcg aacgacagac cgaggcccgt catgtcttct tcgtgcatcg
     3361 gcattgcggc cgggatcggc gtcgcgacat cgaaatcgtc ggtgatgcga cggccgatcg
     3421 cagatagtgg cttcgtaccc gtcggcgtct tcgtgcgcgg cgccgccggg actagcgctg
     3481 ctgctgcctg cgcggctttc gccgtgagcg gacccgacga cggcgtgcgc ttgcgaatca
     3541 cgagccccga ttcgggcgcg acgggaatct gatcgggtgg caccggaacg ggctcgggca
     3601 gcggtggctt gatcgcgacg gcgatcggcg caccactgag gcgtgctgcg agatcgatcg
     3661 ggcccgacga cgacgttcgc gcacgtgtca tgggatcgac ctcggtcggt ccgtcgtccg
     3721 gctcgcgcga tggcgatttg gtgcgcgcga tgaccggttc gttcggcgac accgtacgcg
     3781 caatcatggg ctgttgttgc gacgcggtct tcgtgcgcgg tgcgccgacc gacgttcccg
     3841 tgccggtcgg tgaatccgac gcggtcttcg cgcgttcacg agccgctggc tgttgcgacg
     3901 gcgccttcgt gcgctcgctg tcggtgccag gccgcttgct tgcgagcgga ggcgtatcgg
     3961 tcacgcggtc gcgcgtcgtc gctggcgcga cgcgttcgcc cgtcggcgta cgaccagagg
     4021 cgacgcgctc acccgttggc gtgcgacccg gcagcgggat gcgctcgaac gacgcgggcg
     4081 tcggccggcg ctcgcgcggt gcgggttcga tctgcttggt ttggatcggc gtgaccggca
     4141 ggcgcgcatc gagatccttc ttgagcacga ccggcttgta ctcgcccgct tcatcgacga
     4201 cggcatcgag ttgcgcgtac cgcagcgcga gcaatgcgaa caggcagccg gcgagcaaca
     4261 tcggcggcgc gaacgtctga ttgaccgttg cgcccatcat gccgagcatg ccgtcggtct
     4321 cgggggcgaa cagatagccg gcgccgaacg cgcacaggaa cacggcgctc gcgagcacgt
     4381 agccgagccg gctcgcgctg acgcttgccg agcccgcgag tactctcgcg aagcactgcc
     4441 cgatcacgag caacccaaga gcgagcgcgc cccagaacgc ggcgatcgca gacaccggat
     4501 acaagccttg catcgacgcc aagtcgatcg tcttgcaggc ctcgctcggc gcgcacatgt
     4561 gcatctcgcg cagatcgatc gtgatctgcg cgggcccaaa gtccacgcgg aaccattcga
     4621 gcgcgaacaa cgcacccgcg atcagcgcca ccgacgctat cgagcagagc agcaaccgga
     4681 cttggtgcct gctcatttgt agattcaggg tatcactcct gcggctggcg cgatccgcca
     4741 accggggttc acccggttct gcgttggggt agcggcaggg tcatggcggc gcaggtgttg
     4801 cggccaccgt tatcggcccg acatcgaggg tttttccggc ctcgaggttg agcccacgct
     4861 tcgacgtcgg ccgcggcggc gtcatgacca tcaggatgct cgggcccgcc ttcgcatcga
     4921 ggcggaacgt gccgtccggg ttgctggttg gcggcatgcc gtggagctcg acacgtagct
     4981 ggccatcgcc ggtatccgga atgatcgtca ccggcacgcc accgaccggc ttgccggccg
     5041 catcgatcag cttgccgacc acgatcgcgt tggcagcgag gttgatgtcg acatgcgctg
     5101 tttgatcagg ctgcactttc accgtcgcgc tgccattgcc ggcgctcgac gttacagtga
     5161 ccttgtactc gccaggatcg acgcgcgcga acgagaacgt gccgtccgcg gtcgtgaagc
     5221 tccgctgtgc ccgcgtcgga ccttcgagct cgacctgaaa cgaggacggc attgccgcgc
     5281 cgccggtgac gagcttgcct tcgagcttcg tgagcccgac cgcttgcagc gtgatctgag
     5341 catcgggcac gaccttcgtc gcgcgcccac gcagcttgcc agcctgcgct tcggcgacca
     5401 cggtccacgg cacgcgcgcg aggtttctga tctcgaactt gccattcgca tccgtgagcg
     5461 cgggcggcat gccgccggtg tcgccgccac cgtcgtcgtg cgcctcgatc atcaccgagc
     5521 gcgagccgcc ttcgtcagga ccatgattgg cgagtgcctc gctgagcatg tcctcgagat
     5581 tctgattgac cgatacccac gcgtcggcaa tcggcttgcc gtcggagcca gtgacgacac
     5641 cgcggatcac gccatcggga cgatcgaccg tcagcgtgac gcccgtcttg tgctcggttg
     5701 cggtgaccgc gatcttcgcg tccggcttca tcggcagcgg tcgaccgcga tcgagcacgc
     5761 gcaaccgata ggtgcccgcg gcgagcccgc ggagctcgaa cgcgccagtc gcgttcgtga
     5821 gtgactgtac gcccgacgtc accatgccgt tgacgatcat cgtgcgctcg gtgccgccgg
     5881 ttgctgcagc catcacggtg acgccgccga tcggttttcc gccaccgtcg agcaccttgc
     5941 ccgcgatcga cgcgccgggt ttcacctcga tgacgacatc tggcatcccg ggcttgacgt
     6001 cgatcttctt cgtgccttgc tcaccgctcg ggcagcgcgc cttcaagtca tatgcgatcg
     6061 gatgcgcggg accgacttcg aattcaccgt cgggaccggt cgtgatcggc gcgatcaaca
     6121 tcggcatgtc gccgcgttgc atcgcccctt cgtcgaagtc gagctggacc tcgcagacct
     6181 gccggcgctc gacatggcct ttgatcttga ggccgcgccg cacggtgatc ttcacaccgt
     6241 cgagatcttt gtgctcgagc tccacgttcg tggtgccggc tggcaagaac tcctcgctgc
     6301 gcgccatcag cgtgtacttg cccggcgcga gtccttcgag cgcgaactgt cccttcgcat
     6361 cggccttggc atcggcgttg tcgccgggcc cgctaaacgc gttgacctcg acgttcggcg
     6421 ccggcgcacc ggattcatcg accaccacac cgcgaatcac cggcgctcgg ccgacgacga
     6481 tctcgacatc agtgacctgc tcggcgacac cgagcccgac gatcgtgggc gcttgcgacg
     6541 cgcgtccgac ggcgcgcgca ttcagctcgt agccgcccgg acggaggccg gtgatccgga
     6601 accggccgtc ggctccactc ttcacatcgt gcgttgcgcg ctcgccgagc atcatcgcgg
     6661 gggcatcgcg ctgggcgagc accgtcgcgc cgggaactgg ttcgcgcgtt tgctcgtcac
     6721 gcacgacacc ttcgatcacg ccgccgggta cgagttggaa atcggcggat gcgccgctcg
     6781 caccaacctc gacgaagcga gattgcggcg cgtagctcga ttcgctcgcc gcgacgagca
     6841 gctggccctc ggcgacggac agcttgtact tgccgtcggc gccggtcagc gttgacgcga
     6901 ccgcatcgct cggtctcgcg acggcaccga gctttgcggc atcgatgcgc gcgcccgtga
     6961 tcggtccgcc cgaagcgtcg gtgacggtgc cggtcagggt gcgcccaccc ggcgcgagct
     7021 tcaggtcgac attgacgacc tcaccggcgc gcaactcgcg cggccgcagc gctgcgggct
     7081 cgtgcccatc ggccgacgcg ctgacctgcc acgtgcctgg ctcgaggcgg tcggcacgcg
     7141 cgacgccatc cttgccggtc tcgacgatgt cgacgtcacc gcgctcgcgt tcgaagcgaa
     7201 cggtcgcgcc ggcgagcggt cctttgtcgt cggtgacggc gaccacgagc ttcgcgggat
     7261 ctgcgggctc cggcgctgcg tgctgcgcgt gctgtgcaga ccccttcgtc gcgactgccg
     7321 gggcaggggc gcgctcgtcg cgatggcgcc gcatatagag gaagaggccg atcgcggcga
     7381 tagccacgat cgacatgatc aatagacccc ggctcttgtt catcgcggcc ctcaaccggc
     7441 actcgggccg gcgaattccc tacaataggc acgaatgcgt gcgctgtgga ttctggcctt
     7501 cgttggatgc gcgagcggtc ctccgtatca actcagcgtg cataacagct acgaggttgg
     7561 tgaggacccg acgatcggcg ttgcgatcgc acaaccgacg caagaaaaag cggttttgat
     7621 cgtcacgcgc cccgatggct cgacggtgcg acagcgagtg tcgctgggtg tcgcgcgcaa
     7681 caacattcgc ttcggcggca ccgtcgtcga caagagcacc gaagtcacct ttacggagcg
     7741 cggcgactat cgcgtcgagc tgcggaacga caacgcgcta ttagccaagc aagagatccg
     7801 tatctcggtc gatcagttga cacagctgtt cgacgattcc gagatcgccg atttcgcacc
     7861 ggtcgttcgc tacacgcggt cgcgcgggct caacaagcag cagtggaaaa cgtacggcgc
     7921 actctacgag cacacgctgc gcaagggcgt gcagatccag gtcctcatcg aggatccagg
     7981 cgatgcgctc gacgaggcgt ggaaacaata caaagaggaa ggcacgctag gcgtcatcga
     8041 gaacaactac gttcggtttc gggatcgcaa cggcagcgtg tctgcgagct ggatctcggg
     8101 caagaagatc gtcgcgatgc gcgccgagac gctggacgat ttcgagcgtg gcttcatcgg
     8161 cgcgttcctc gcgcgctttc cgagcaagct ccgccactag cccgagagca cgctgaccca
     8221 gacctggatc gcgcgggcat cgatgcccgg gtccatcaca tcgcttcccg cgaacagctt
     8281 gccgaacacg aacgacgtcc cgagggtcca gtgcttgtcg accgcgtacg tcgcggacgc
     8341 gccgagcgga atggccatgc gatccgcgaa gtgtttgacg gggcctttga cgccggtgcc
     8401 gatgccgagc gcgagcggcg ccactggttt gacaaagagg ccgatcggca cgaacagctg
     8461 atcttcgtgc ggcacgacgc gatcgtaacg atcgtcgagc gcgagccaca cactcggaga
     8521 gaccaacgcg aacagcttgc cctcggacat cttgagcttg aagccgagct tgagatcggt
     8581 gtgcaccgac ggctcgatcg cggaccacat cacaccggcg ttcagcgcga acgcggccga
     8641 tcctgtcgcg atgctgacta gaccctcgat gccgccgccg ttgaatggca cgcggcagct
     8701 gcggtcgacg ccgcccatgc agatgccagc gccagcgccg ccgcgaaagc cggtcagcgc
     8761 cgagcccgag gtgttcgccg agagcgtgaa cgcatcggtg acgccgaatg acaaatcagg
     8821 tgcgatcgat gccggcgcgg cgaccggcgc tccactcgtc atgcccatct cggcggtaac
     8881 cgatgcctgg atcgcgttcg agcgcagcgt cagcgacgcg ggtcgtggcg gtggtgcaac
     8941 cggcggaggc gcgagcgcgc ggatacgcat cggcggctcg gtatggacac gcgctgcctc
     9001 gacgtgcgga cgcacgtggc ggcgacgttc cgcggatgcg atcggcacga gaacgacgag
     9061 cgtggcgagt gccgcgtaca acctcatcac gcaacaacga tcgcacgcac gcgcacgcga
     9121 atcatcgggc tagggtttcg taggcaaggt cacgggctag atctggctta ccccgtgaga
     9181 catcgacgga gttgtcgtca gaaatgtccg gcgagcgaga caccgacgga gctggtggtc
     9241 gcggtcggcg tgatgacgac acgttctttc ggtgccgtgt accagaccgc cgccgcgccg
     9301 acgatcgcga gcacgctgcc ggtgaggacg atcttcgccg cgatctcgtt acggtgcgcg
     9361 agtgcgagct tgtcgatcgc gacctgctgc gcggtcggat tttcacacgt gccctggtag
     9421 ttgcggtcga gcgtgcagtt ctcgat
//
LOCUS       DASUFX010000373         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_338581,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000373 DASUFX010000000
VERSION     DASUFX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338581"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VFV99_14910"
     CDS             <1..1404
                     /locus_tag="VFV99_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5060656.1"
                     /translation="AEERQLFDPAYQGSVEQRLARQQYRKDLERIVVEAARQGAAPAA
                     ATLATLRSERGVEESEEERALAEILAPGGPIAAIYTEELAAIKRLVEAAEAAHPDASK
                     SDASKGASESASLALLHHLATRRAHEHAIHALGIVAVMTKKSDADTLRQHAKSRGGIE
                     PRRLAVLDGVDASLRDPLVELVTRLGRCAHSELRADPILAIADDASRHVRAAVAVLLS
                     RFDDDRSRTQLLSMLDDVEPIVREAVVRAMGAKSRLTRELLSKVLNDPSPLVRHSAVR
                     AVSRTTSGELPAVDPAVLAQTTAGVGKPGVYATLDANAAMASLTTIEKMMLIRQVPIF
                     AELDAEDLEELASIVEERRINAEKDLFKEGDVGDAVYLIVKGRVRVFVGGGSRPEKTI
                     NELGPGSCIGEMAVLDASPRSATVRALDPTRALRVPGEGFKRVMSERPEMSEAIVAEL
                     VRRMRGMMAQATKGDPP"
     gene            1412..2422
                     /locus_tag="VFV99_14915"
     CDS             1412..2422
                     /locus_tag="VFV99_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060657.1"
                     /translation="MRATFLVIVLSSACYSPGYRDCEITCASQQCPSGYSCNAGGFCV
                     TDPQMSCSGIGSDGGGSDVMPDMGMQGSGTWNPPMPVPFTAGSPTGLDDQPSLTDDMR
                     TLYTRRIVGGQSELYSSTRQLQTDPFPTPAIVSSISTAAANEITPELGGNGLTIFYGS
                     DRGASPPQFDIWFASRNQISGNWQTQTTPVQGLASTAADESPTVARDGLTMVFCSLRM
                     SGDAGDIYLSNRATQADPWGAPMPMTAINNMGRRDEGPFLSTDKLTIYFSSDRPGGTG
                     GFDLWQATRPDLSSPFGMPTPIMELNTSGNERDPWVSPDGKRIFFTGMLNNVPTIMQA
                     TR"
     gene            2436..2705
                     /locus_tag="VFV99_14920"
     CDS             2436..2705
                     /locus_tag="VFV99_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060658.1"
                     /translation="MKRDATNVIWRTVVFAGAMLGAPACSKKHPQTTPTNTATPTDPA
                     ATQPTQATEQKTDTPDPCAGDPCAGRPRGADDDGGGGAGRGFVLS"
     gene            2752..3204
                     /locus_tag="VFV99_14925"
     CDS             2752..3204
                     /locus_tag="VFV99_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060659.1"
                     /translation="MAKHRDLARELYASSLGRKSASTIWRTVVFAGAMLAAPLTGCGG
                     SPKKTTTQPMTGGDAAQPSDQAAQDKAAADKAAEEKAAADKAAAEQAAAAAEAEKAAA
                     DQKAQEEAEAKAKEEADAKAKADADAAAKKRPRGGGTARPKGRGFVLA"
     gene            3208..4128
                     /locus_tag="VFV99_14930"
     CDS             3208..4128
                     /locus_tag="VFV99_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5060660.1"
                     /translation="MNVEGDDGPSQRDTVDDESRDEPKRGDDGYEPTSPWWLSGTPLE
                     AEHHRLMRRRATPIPWQATTAGTLTDAERKRLAGTWMRRSEAEYLAVSTFSVLAIDLV
                     AAMAPADVISLCLRAGIDEVRHAEMCVRMAQIYSGEQNLPVPGMSSLPDDPSRPKLHQ
                     ALANTMLVSCVSETYATTVLSATRDLTTDPTAQAVLTAIYSDEVMHARLGWSYLRHAI
                     EVGGQGVIDAAAEMLPRALRGVANVVERERPEGEVTEAVRAHGLMTPAEERVIYATCV
                     REVLVPGFQALGIPVGSALEDYGDAWAHAA"
     gene            4110..4721
                     /locus_tag="VFV99_14935"
     CDS             4110..4721
                     /locus_tag="VFV99_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060661.1"
                     /translation="MGARSLIGLTSLAALGFGACGRHQPPATPEPAKPASTPEKVVAD
                     FEQSVKTSKDAYVGMFDFVAVGEFEILLHRYDLNGRLKNLPDDMKQQFAAEDGTPYPP
                     ERERRNVGNFYTFLAQRTVGTGGCTLSEPRTHYGKLLGVQFDPLPEGTSPGYEKLRTT
                     VNAYLAKGGVVGIRCSGGNGGLALVWTERPNQRGYDLITIYDD"
BASE COUNT          878 a         1561 c         1652 g          717 t
ORIGIN      
        1 gcggaagaac gccagctgtt cgacccggcg tatcagggtt cggtcgagca gcggctcgcg
       61 cgccagcagt accgcaagga cctcgagcgc atcgtcgtcg aggctgcacg ccagggcgcg
      121 gcgccggcgg cggcgacgct ggctacgctg cgcagcgagc gcggcgtcga ggagagcgaa
      181 gaagagcgcg cgctcgccga gatcctcgcg ccgggcggac cgatcgcggc gatctacacg
      241 gaagagctcg ctgcgatcaa acggctcgtc gaagccgccg aagccgcgca tcccgacgcg
      301 tcgaagagcg acgcgagcaa gggcgcgagc gaatcggcga gcctcgccct cctccatcat
      361 ctcgccaccc gccgcgcaca cgagcatgcg attcacgcgc tcggcatcgt cgcggtgatg
      421 acgaagaaga gcgatgcgga cacgctgcgc cagcatgcga agtcgcgcgg cgggatcgag
      481 ccgcgccggc tcgctgtgct cgacggcgtc gatgcgtcat tgcgcgatcc actcgtcgag
      541 ctcgtcaccc ggctcggcag atgcgcacac tccgagcttc gtgccgatcc gatcctcgcg
      601 atcgcggacg acgcgtcacg ccacgtgcgc gccgccgttg cggtgctgct ctcgcgtttc
      661 gatgacgacc gatcgcgcac gcagctcctc tcgatgctcg atgacgtcga gccgatcgtg
      721 cgtgaggcag tggtgcgcgc gatgggtgca aagagccggc tcacgcgcga gctgctgtcg
      781 aaggtgctga acgatccgtc gccgctcgtg cgtcattcgg cggtccgcgc ggtgtcgcgc
      841 acgacgtcag gcgagctgcc ggcggtcgat cctgccgtgc tcgcgcagac gacggccggc
      901 gtcggcaagc ccggcgtcta cgcgacgctc gacgcgaacg ccgcgatggc ctcgctcacg
      961 acgatcgaga agatgatgct gatccgacag gtcccgatct tcgccgagct cgacgccgag
     1021 gatctcgagg agcttgcatc gatcgtcgag gagcgccgca tcaacgcaga gaaggatctg
     1081 ttcaaagaag gcgacgtcgg cgacgcggtc tatctgatcg tcaaaggtcg cgtgcgtgtg
     1141 ttcgtcggcg gcggcagccg acccgagaag acgatcaacg agctcggccc cggttcgtgc
     1201 atcggcgaga tggccgtgct cgacgcatcg ccgcgctcgg cgaccgtccg cgcactcgac
     1261 cctacgcgcg ccctgcgggt ccccggcgaa ggcttcaaac gcgtgatgag cgaacgcccc
     1321 gagatgtcgg aggcgattgt tgcggagttg gtgcgccgaa tgcggggtat gatggcgcag
     1381 gcgaccaagg gtgatccgcc atgagcccat catgagagcg acgttcctcg tcatcgtgct
     1441 gtccagcgct tgctactcgc cgggatatcg cgactgcgag atcacgtgcg catcgcaaca
     1501 atgcccgagc ggctattcgt gtaacgcggg cgggttctgt gtcaccgatc cgcagatgag
     1561 ctgtagcggc atcggctccg acggcggcgg ctcggacgtg atgcccgaca tgggcatgca
     1621 gggcagcggg acgtggaacc ctccgatgcc ggtaccgttc accgcgggaa gtccgaccgg
     1681 tctcgacgat cagccgtcgc tgaccgacga catgaggacg ctctacaccc gccgcatcgt
     1741 cggtggccag agcgagctgt acagctccac gcgccagctg caaacggatc cgttcccgac
     1801 accggcaatc gtgtccagta tctcgacggc cgcggccaat gaaatcacgc cggagctcgg
     1861 cggtaacggc ctcacgatct tctacgggtc cgaccgcggt gcttcaccgc cgcagttcga
     1921 tatctggttc gcgtcacgga accagatcag cgggaactgg caaacgcaaa cgacgccggt
     1981 gcagggtctc gctagcaccg ccgcggacga gtcgccaacg gtcgcccgcg atggcctgac
     2041 gatggtgttc tgctcgttgc gcatgtcggg tgatgccggc gacatctatc tcagcaaccg
     2101 cgccacccag gcagacccat ggggcgcgcc gatgcccatg accgcgatca acaacatggg
     2161 acgccgcgac gaggggccgt tcctgtcgac ggacaagctc acgatctact tcagctccga
     2221 ccgcccgggc ggcacgggtg gattcgacct gtggcaggcg acgcggccgg atctcagttc
     2281 gccgttcgga atgccgacgc cgatcatgga gctcaatacg agcggcaacg agcgcgatcc
     2341 gtgggtatcc ccagacggca agcgcatctt ctttaccggc atgctcaaca acgtgcctac
     2401 gatcatgcaa gccactcggt gataggatca aaacgatgaa gcgtgatgcg acaaacgtga
     2461 tctggcgtac ggtggtgttc gctggcgcga tgctcggcgc accggcgtgc agcaagaagc
     2521 atccgcagac aacgccgacg aacacggcga cgccgacgga ccccgcagcc acgcagccga
     2581 cacaggcgac cgagcagaag accgacacgc ccgacccgtg cgctggcgat ccgtgtgcgg
     2641 gacgcccgcg cggtgccgac gacgatggcg gtggtggcgc cggtcgcggc tttgttctgt
     2701 catagcctct agcgctggaa atcgcgcggc cacggtgtca tcgtcagcac catggcgaag
     2761 caccgtgatc tggcgagaga gctgtatgcg agcagtcttg gtcgcaagag cgcatccacg
     2821 atctggcgga cggtcgtgtt cgccggtgcg atgctcgccg cgccgctgac cggctgtggt
     2881 ggcagcccga agaagaccac gacgcagcca atgaccggcg gtgacgccgc gcagccgagc
     2941 gatcaggcgg cgcaagacaa ggccgctgct gacaaggccg ccgaggagaa agctgccgcc
     3001 gacaaggccg ccgccgagca agcagcggct gctgccgagg ccgagaaggc cgccgccgat
     3061 cagaaggcgc aggaagaagc cgaggcgaag gccaaggaag aagcagacgc caaggccaaa
     3121 gcggacgccg atgcggcggc gaagaaacgg ccgcgcggcg gtggcactgc gcggcccaag
     3181 gggcgcgggt tcgttctggc gtagccgatg aacgtcgaag gggacgacgg tccgagccag
     3241 cgcgacaccg ttgacgacga atcgcgcgac gaacccaagc gcggcgacga cggctacgag
     3301 ccgacaagcc cgtggtggct gtcgggcacg ccgctcgagg ccgagcacca tcgcttgatg
     3361 cggcgacgcg cgacgccgat tccgtggcag gcgacgaccg cgggcacgtt gacggatgcc
     3421 gagcgcaagc ggctcgcggg cacgtggatg cggcgctccg aggccgagta cctcgcggtg
     3481 tcgacgttct cggtgctggc gatcgatctc gtcgctgcga tggcgcctgc cgacgtgatt
     3541 tcgttgtgcc tgcgcgctgg catcgatgaa gtgcgtcacg ccgagatgtg cgtacggatg
     3601 gcgcagatct attcgggcga gcaaaacctg cccgtgcccg gaatgagctc gctgccggat
     3661 gatccgtcgc ggccgaagct ccaccaggcg ctcgcgaaca cgatgctcgt ctcgtgcgtc
     3721 tccgagacgt atgcgacgac cgtgctgtcg gcaacgcgcg acttgacgac cgatccgacg
     3781 gcacaggccg tgctcaccgc gatctactcc gacgaagtga tgcacgcgcg acttggctgg
     3841 tcgtatctgc gccatgcgat cgaagttggt ggccagggcg tgatcgacgc ggctgccgag
     3901 atgttgccgc gtgcgctccg tggcgtggcg aacgtcgtcg agcgcgagcg tcccgaaggt
     3961 gaagtcaccg aggcggtgcg tgcgcacggc ctgatgacac cggccgaaga acgtgtgatc
     4021 tacgcgacgt gcgtgcgcga agtgctcgtg cctggcttcc aggcgctcgg cattccggtc
     4081 ggcagcgcgc tcgaagacta tggggatgca tgggcgcacg cagcctgatc ggattgacat
     4141 cactggcagc tctcggcttc ggcgcgtgcg gacgacatca gcctccggcg acgcccgagc
     4201 cggccaagcc cgcaagcacg cccgagaaag tcgtcgctga cttcgagcaa tccgtgaaga
     4261 cgagcaagga cgcgtacgtc ggcatgttcg acttcgtggc ggtcggtgaa ttcgagatcc
     4321 tgctccatcg ctacgatctg aacggccggc tcaagaacct gcccgacgac atgaagcaac
     4381 aatttgctgc agaggacggc acgccctacc cgcccgagcg cgagcgacgc aacgtcggca
     4441 atttctacac gttcctcgcg cagcgcaccg tcggcaccgg cggttgcacg ctgagcgagc
     4501 cgcgcacgca ctacggcaag ctgctcggcg tgcaattcga tccgctgccc gaagggacat
     4561 cgccggggta cgagaagctg cgcacgaccg tcaacgcgta cctcgcgaag ggcggtgtgg
     4621 tcggcatccg gtgtagcggc ggtaacggcg ggctcgcgtt ggtgtggacc gaaaggccga
     4681 accaacgcgg ctacgatctg atcaccatct acgacgactg atcgcgatcc gttataggat
     4741 cgtgcgacgg gtggcatggg gagcgcttcg gtcaagccgg acccgctgat cggcactgtc
     4801 gtcggcgg
//
LOCUS       DASUFX010000374        11247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1728492, whole genome shotgun sequence.
ACCESSION   DASUFX010000374 DASUFX010000000
VERSION     DASUFX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728492"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFV99_14940"
     CDS             <1..364
                     /locus_tag="VFV99_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX5060662.1"
                     /translation="MTNTSLLAGPHGLDEIIKSVKRGVYAKRFSGGQVNISNGDFVFS
                     LSESYLIEDGKLTAPLKGVNLIGNGPDVLRRVDMLGADYQLSDGIWTCGKDGQSVPVN
                     VGTPTVRIASITVGGTQA"
     gene            361..1758
                     /locus_tag="VFV99_14945"
     CDS             361..1758
                     /locus_tag="VFV99_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5060663.1"
                     /translation="MSGAQVTEATVKELCDAAALAVEIAKKAGADHAEVLVRDGSELT
                     AKVRLGEPELVQEAGSRALGLRVLKGAKRAVTYTSDLRRESLEALCKETVALAELAEA
                     DEYALPPDPALLAKEIPELDLYDPAVADVDAAWALREAKNGEAAARRHDARVTNSEGA
                     TWSRVLGATAFATSGGFVGGYRGSYASLVVEPLCDDTTDPENPKKRNGFWWTASRFLD
                     QLQVAEAVGIEAARRTVATLGSRKVETQECPIVFDPEVARSIVGTIFSVANGSSFWRK
                     SSYLLGKEGEVVASPLVTIVDDPLIKRAPGSRPFDGDGLPTRKNNVIAKGVLGPVLCD
                     VYSGRKLGRASTASAGRGIGGNPGPTTSNLIMEAGSVSREELLKQTTRGLYVTSLMGF
                     GFNPVTGDFSRGAQGFWIEGGELTFPVSEVTIAANFDQILKRIDLVANDLELRSSTAA
                     PTFRVSHMTLAGTSK"
     gene            1913..4258
                     /locus_tag="VFV99_14950"
     CDS             1913..4258
                     /locus_tag="VFV99_14950"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060664.1"
                     /translation="MPLAAGGMARIYIGRSTGIGSFERHVVLKMITPERANDLTAVNM
                     FLDEARLAASLNHQNVAQVFEVGEDVGIHYLAMEYVHGQDLRAVLAKAGSQGTRIPLE
                     LALTVVAGAAAGLHHAHERRGPDGAPLGIVHRDVSPSNIMIGYDGAVKLLDFGIAKAT
                     ARSVETQSGIIKGKFAYMAPEQCRGRDVDRRSDVFSLGIILYEISTQHRCFRADSDFD
                     TMHRIVTGDVVRPTRLVQNYPQALEAIVMKALAVDANQRYQSSGLLLEALESFAVSSR
                     MSLSTMGLGRFMRDMFGEVQEPWLNTAATKVHQPLTPKESTISSTNGQGSKSAPIMLP
                     PEQLAPVDTSGIPDEEARFADSDGINSMAKTTLDLPREGDREPDENASDEWNAKSYPA
                     PKPSMASDDTIPPDPVGRQSYPQGIPPPPQVQRKSQPQVPAQPEPRPTPNPALPMTYP
                     KASSLPPGELPLPPPAPPQHQFNVPGSGSGPSHPSHPAIGIVSTKHGYTSGAMTRGDA
                     SYPRYDAPGDVSSSPDLQLTPNRRPLFIGLGIVLVGIIVMLVVSFTGGDEAPAPRPAD
                     EPAQPMEEHATTPEQTQPAPGSNTDVADENMVSVIIHSDPVGADVLIAGTKIGTTPFE
                     SKLKRGTKIAQLTVQKDGYAPFNTKIDLGGEYENRKIKLTKLEDLPKGSDGNPMSPDN
                     GEESTPKGTPKTGTEKTGTTPKTGTEKTGTPKAGTEKTGTKTGTEKTGTSTKTGSEKT
                     GTKTGTKTGTGTGTTTPKVEKEKCQPPGPNVDPFGPPICKT"
     gene            complement(4386..5021)
                     /locus_tag="VFV99_14955"
     CDS             complement(4386..5021)
                     /locus_tag="VFV99_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEX5060665.1"
                     /translation="MARRVAAELRDGFYVNLGIGMPTLVANFIPPGIDVVLQSENGLL
                     GIGPFPYEGDEDPDLINAGKQTVTTIKGSAFFDSAQSFAMIRGGHVDVSVLGAMEVSQ
                     SGDLANWMIPGKMIKGMGGAMDLVAGSRRVIVMMEHTTKEGAPKILKECTLPLTGVAC
                     VHTLVTDMAVIDVQNGGLVLRERAPGLSCDDVAKATGATLTWKGDVPEIAI"
     gene            complement(5045..5746)
                     /locus_tag="VFV99_14960"
     CDS             complement(5045..5746)
                     /locus_tag="VFV99_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX5060666.1"
                     /translation="MHKVYPDAQAALEGVFDGAVILAGGFGLSGNPENCIAELARRQV
                     KDLTIVSNNCGTTEKGLGVLLAQGQVKKMISSYVGENKIFEKQFLSGQLQVELCPQGT
                     LAERLRAGGAGIEAFFTPTGFGTKVAEGKETRTINGKQCVMEHAIRGDFAIVKAWKGD
                     KWGNLVFRKTSRNFNPLCAQAGKITIVEVEQLVEVGELDPDHVHLPSIYVQRIFQGTH
                     YEKPIEQRTLRKRSA"
     gene            5772..6557
                     /locus_tag="VFV99_14965"
     CDS             5772..6557
                     /locus_tag="VFV99_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX5060667.1"
                     /translation="MPIITLTTDFGTRDGYVGAVKGVIHRVLQSTVVRPVWEHTSVVD
                     IAHDLPRGDIAHAAWVLATSTIEFPEGSIHVIVVDPGVGGARQNVVVESAGGQLYVGP
                     DNGVFALLGDTSAHVVTKTALLAPKISSTFHGRDLFAHVAARLACGYRPTDFGKATRL
                     VGTLPWGRRPEGVGRIIHIDGFGNLISDLPAREAGGTVLFETRHIPVMRTYEDVPVGE
                     LLAYVGSAGTIEIAVRDGRADALLGVQRGAKVLPDPDATGPYR"
     gene            6578..7765
                     /locus_tag="VFV99_14970"
     CDS             6578..7765
                     /locus_tag="VFV99_14970"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5060668.1"
                     /translation="MGAEIEVEITSLAAGGDGVARDANGRVTFVPRSTPGDVVRVTLT
                     KQTKSFARGTIVEIVRRSPQRINPPCPYFVAGCGGCQWQHVERAGQLAAKHAIVTNAL
                     RHLPGVVVEDVLDPAPAYGWRRRARFHGAHGLVGLYEHGSHTLIRLQQCPQLEPELEP
                     ILKELNALRPPEGEVALLRGHTGDIVLGVEHAWRGAAKLVGRVGIVGVVAGNETHGTT
                     VIEIEPGLFGGPWDFAQASRAGNAKLIEVARAAVGAAGGVNSSTSKRLLELHAGAGNF
                     TRTFVADGWDVLASDVHAPARPIAGARFEGGTAEDVLARAAGPFDAIVLDPPRTGAVE
                     AMPGIAKHAPPVVVYISCDPATLARDAAKLLEVGYRAERAWPVDLMPQTSHVEVVLRL
                     TKG"
     gene            complement(7762..8283)
                     /locus_tag="VFV99_14975"
     CDS             complement(7762..8283)
                     /locus_tag="VFV99_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060669.1"
                     /translation="MIRLFVVLALLGGVAHAETIVSLPNSKATLTLPDGWNRVKHSFK
                     GVVEIFKHDNGATLVVTRADVPNPDAWISDKKQAYVDKVESGIKAGVPGYKRRAKKIV
                     DANGVPALDLETKRDGGSTIVVRVLLFRTYSVSAAIEVPKGVDVAAARVVVKTFAPPK
                     EPKPEPAPTKKAG"
     gene            complement(8280..8927)
                     /locus_tag="VFV99_14980"
     CDS             complement(8280..8927)
                     /locus_tag="VFV99_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060670.1"
                     /translation="MRSKHASASSPETGRAKDAKETSYRHEDPRRAPLETVEVELEVS
                     RQRWISWTIGGIIGGALLIWKLGTVGVWVGYVLIALGLFRGYQLAMSFLHPPGKFVIS
                     EDKVSLPRGVHRARPLVLEPSAVTAAYFLRRSVPWNRSAPVLVVELGDKAMAFPRDWF
                     ASEADQRHVIHALLGLRPRGDAPADKADGKSDAKSDAKSDTKSDAKSDAGPVTES"
     gene            complement(8878..10254)
                     /locus_tag="VFV99_14985"
     CDS             complement(8878..10254)
                     /locus_tag="VFV99_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5060671.1"
                     /translation="MTNTLLVTADRAITEPAGFAGALGSVTRGGGARGPTAILAIDGE
                     IVLVGDPGDVAADPRAAGARRLDWSHRAVVPGTVNTHNHSFQSLLRGIGDDLPFLEWR
                     DRALYRYSPRLTADDMGTAALFAFGEMLLHGVTTVCDFYYLNAQGNDHASATIEAARR
                     LGMRIVLARCFYDWDGAPAAYRESIPQAVANYQALHARYRDKVRHLVTIQPAPHSQHG
                     ATPAMIEAGAGCAADAGEPWHIHLAEEKYQVDQSLERFGKRPIHAVAGLQVDMSGMIA
                     VHGCWFDESERSLLAERGGSLAYCPGSNMFLGDGVTDLVDLVARGVRVGLGTDGGCSN
                     NRVSVFDEMRMAALLQKVHKTDGQAIAAETCFALGTRTAGEVLRLPIGRITPGYRCDL
                     VALDLDDPSLWPSQALEKNVVYALSPRAITDVVVDGQEVVTARRLCHVSLDEIQARVS
                     ELTRDWSR"
     gene            10298..>11247
                     /gene="holA"
                     /locus_tag="VFV99_14990"
     CDS             10298..>11247
                     /gene="holA"
                     /locus_tag="VFV99_14990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5060672.1"
                     /translation="MQELIEKGLAPIYVFHSEHPILIERAVATIRDAAVPPASRGFNY
                     DVIEGKPKGNQIVALAQTLPMMAKHRMVFVRDLGLLPADEAEPVINYLSKPNPSTVLV
                     AITSKLDKRLKLFAQLSKKGFLHVLEAPRQVGPWVREEAKLKGVRIDSAAMSRLVDTV
                     GNDLSRLSLAIEQLGLYAGDRPVTSDDVDELIADTRERSVFELTDAIGAADRGRALAA
                     VASLCDQRESAVGVVVMLARHIRQLSMLHTCRMHGVPRSEWNVRIGVPPFVVDKLVAQ
                     AKSYTPGALATATQRLANADRALKGDITLTTQTAAFTGPQL"
BASE COUNT         1905 a         3679 c         3824 g         1839 t
ORIGIN      
        1 catgacgaac acgtcgctgc tcgctgggcc gcacggcctc gacgagatca tcaagagcgt
       61 caagcgcggc gtctacgcga aacggttctc cggcgggcag gtcaacatct cgaacggcga
      121 cttcgtgttc tcgctcagcg agagctacct gatcgaagac ggcaagctca ccgcgccgct
      181 caagggcgtg aacctgatcg gcaacggtcc cgacgtgctt cgccgcgtcg acatgctcgg
      241 cgccgactac cagctcagtg atggcatctg gacctgcggc aaggacgggc agtcggttcc
      301 ggtcaacgtc ggtacgccga cggttcgcat tgcatcgatc accgtcggag gcacgcaggc
      361 atgagtggcg cgcaggtaac agaggcgacg gtcaaggagc tgtgcgacgc ggcggcgctc
      421 gcggtcgaga tcgcgaagaa ggcaggcgcg gatcacgccg aagtgctcgt gcgcgacggt
      481 agcgagctca ccgcgaaggt gcggctcggc gagcccgagc tcgtgcagga ggcgggcagc
      541 cgcgcgctgg gtctgcgcgt gctcaagggc gcgaagcgcg cggtgacgta caccagcgat
      601 ttgcggcgcg agtcgctcga ggcgctgtgc aaggagacgg tggctctcgc tgagctcgcc
      661 gaggccgacg agtacgcgct gcctccggat ccggcgttgc tcgcgaagga gattcccgag
      721 ctcgacctct acgatcccgc agtcgcggat gtcgacgcgg cgtgggcact gcgcgaagcg
      781 aagaacggcg aagcggcagc gcggcgccac gatgcgcgcg tgacgaacag cgaaggtgcg
      841 acgtggtcgc gggtgctcgg tgcgacggcg tttgcgacga gcggtggttt tgtcggcggc
      901 tatcgcggca gctacgcatc gctcgtcgtc gagccgctgt gcgacgacac taccgacccc
      961 gagaatccga agaagcggaa cgggttctgg tggaccgcct cgcgcttcct cgatcagctg
     1021 caggtcgccg aagccgtcgg catcgaggcg gcacgccgca cggtcgcgac gctcggctcg
     1081 cgcaaggtcg agacgcagga gtgtccgatc gtgttcgatc cggaagtcgc gcgctcgatc
     1141 gtcggtacga tcttcagcgt cgcgaatggt tcgtcgttct ggcgcaagtc gagctacctg
     1201 ctcggcaagg aaggcgaggt cgtcgcatcg ccgctcgtca cgatcgttga cgatccattg
     1261 atcaagcgcg cgccgggtag ccggccgttc gacggcgatg gcttgccgac gcgcaagaac
     1321 aacgtcatcg cgaagggcgt gctcggaccg gtgctgtgcg acgtctactc gggccgcaag
     1381 ctgggtcgcg cgtcgaccgc gtcggccggt cgaggcatcg gtggcaaccc cggccccacc
     1441 acgtcgaact tgatcatgga ggccgggagt gtgtcccgtg aggaactgct gaagcagacc
     1501 acacgcggcc tgtacgtaac ttcacttatg ggatttgggt tcaatccggt gaccggagac
     1561 ttctcgcgcg gcgcgcaggg cttctggatc gaaggtggcg agctgacgtt cccggtaagc
     1621 gaagtgacga tcgcggcgaa ctttgaccag attctcaagc ggatcgatct ggtcgcgaac
     1681 gatctcgagc tccgaagctc gacggctgcg ccaacgttcc gggtgtcaca catgaccctc
     1741 gctggaacct cgaagtagcg ccagtaagtg aagcacgtac atggctgaga tcgttggttg
     1801 agtacctcgg gtctgcttga cttgaacctc ccggaatgaa acgatcttcg acgtgcctgc
     1861 cgtagcaacg gccgcgggac ttccgtctcg cctcggcaag tacgagctgc tgatgccgct
     1921 cgcggccggt ggcatggctc ggatttacat cgggcgttcg actggtattg gctctttcga
     1981 acgtcacgtt gtgctgaaaa tgatcacgcc ggagcgggcg aatgatctga cggccgtgaa
     2041 catgttcttg gacgaagcgc gtctcgccgc ttcgctcaat catcagaacg tcgcgcaggt
     2101 gttcgaggtc ggtgaggacg tcggcattca ctacctcgcg atggagtacg tgcatggcca
     2161 ggatctacgc gccgtgctcg cgaaggccgg cagccaaggc acgcgcattc ctctcgagct
     2221 cgcgctgacg gtcgtcgccg gcgcagcggc aggtttgcat cacgcgcacg agcgtcgcgg
     2281 gcccgacggt gcgccgctcg gcatcgtgca ccgcgacgtg tcgccgtcga acatcatgat
     2341 tggctacgac ggcgcagtga agctgctcga cttcggtatc gcgaaggcga ccgcgcgttc
     2401 cgtcgagacg cagtccggaa tcatcaaggg caagttcgcg tacatggcac cggagcagtg
     2461 ccgcggtcgc gatgtcgatc gccgcagcga tgtgttctcg ctcggcatca tcctctacga
     2521 gatttcgacg cagcatcgct gcttccgcgc ggacagcgac ttcgacacga tgcatcgcat
     2581 cgtcacgggc gacgtcgtgc ggccgacgcg tctcgtccag aactatccgc aagcgctcga
     2641 agcgatcgtg atgaaggcgc tcgcggtcga cgcgaaccag cgctaccaga gctcgggcct
     2701 gttgctcgaa gcactcgaga gcttcgcggt gtcgtcgcgg atgtcgctgt cgacgatggg
     2761 ccttggccgc ttcatgcgcg acatgttcgg cgaggtgcag gagccgtggc tgaacacggc
     2821 cgcgacgaag gtgcaccaac cgctcacgcc gaaagagagc acgatctcgt cgacgaacgg
     2881 gcagggcagc aagtcggcgc cgatcatgtt gccgccggaa cagctcgcgc cggtcgatac
     2941 cagcggtatc cccgacgagg aagcgcgctt cgccgactcc gacggcatca acagcatggc
     3001 gaagacgacg ctcgatctgc cgcgggaggg agaccgcgag ccagatgaga acgcgtcgga
     3061 cgagtggaac gcgaagtcgt atcccgcgcc gaagccgtcg atggcgtcgg acgacacgat
     3121 tcctccggac ccggttggtc ggcagtcgta tccgcaaggc atcccgccgc cgccgcaggt
     3181 ccagcgcaaa tcgcagccgc aggtgccggc gcagcccgaa cctcggccga cgccgaatcc
     3241 ggcgttgccg atgacgtatc cgaaggcgag ctcgctgccg ccgggcgagc tgccgttgcc
     3301 gccgcccgcg ccgccgcagc atcagttcaa cgtgcccggc tcgggctcgg ggccgtcgca
     3361 tccatcgcat cccgcgattg gtatcgtcag cacgaagcac ggctacacga gcggcgcgat
     3421 gacgcgcggc gatgcctcgt atccgcgtta cgacgcgccg ggtgacgtat cgtcgtcgcc
     3481 ggatctccag ctcacgccga atcggcgccc gctgttcatc ggcctcggca tcgtcctcgt
     3541 cggcatcatc gtgatgctcg tcgtgtcgtt caccggcggt gacgaagcgc cggcgccgcg
     3601 tccggccgat gaaccggcgc agccgatgga agagcacgcc acgacgcccg agcagacgca
     3661 gccggcgcct ggcagcaaca ccgacgttgc cgacgagaac atggtctcgg tcatcatcca
     3721 ctcggatccg gtgggcgctg acgtgctgat cgcgggcacg aagatcggta cgacgccgtt
     3781 cgagagcaag ctcaagcgcg gcacgaagat cgcgcagctc accgtgcaga aggacggcta
     3841 cgcgccgttc aacacgaaga tcgatctcgg cggcgagtac gagaaccgca agatcaagct
     3901 gacgaagctc gaggacctgc ccaagggcag cgatggcaat cccatgtcgc cggacaacgg
     3961 cgaggaatcg acgccgaagg gcacgccgaa gacgggcacc gagaagaccg gcacgacgcc
     4021 gaagacgggc acggagaaga ccggcacgcc gaaggccggc acggaaaaga ccgggacgaa
     4081 gaccggcacc gagaagacgg gcacgtcgac gaagacgggc tccgagaaga cgggaacgaa
     4141 gaccggaacg aagaccggca ccggcacggg cacgacgacg ccgaaggtcg aaaaagagaa
     4201 gtgccagccg ccgggcccga acgtcgatcc gttcggcccg ccgatctgca agacgtaaca
     4261 gcgctgccgc cctttcgcgg cacatcgccg ctccacgcga gcggaaatgg gatcagactc
     4321 ctttttccgg cacatcgccg ctccacgcca gcggatatgt ggtcagacgg tcagccccct
     4381 ttgggttaga tcgcaatctc cggcacgtcg cccttccacg tgagcgtcgc gccggtcgcc
     4441 ttcgcgacgt cgtcgcacga caggccaggt gcgcgctcgc gcagcacgag gccgccgttc
     4501 tgcacgtcga tgacggccat gtcggtcacg agcgtgtgca cgcatgcgac gccggtgagc
     4561 ggcagcgtgc actccttcag gatcttcggc gcgccttcct tcgtggtgtg ctccatcatg
     4621 acgatcacgc ggcggctgcc ggcgacgaga tccatcgcgc cgcccatgcc tttgatcatc
     4681 ttgccgggga tcatccagtt cgcgaggtcg ccagattgcg agacctccat ggcgccgagc
     4741 acgctgacgt cgacgtggcc gccgcggatc atcgcgaacg actgcgcgga atcgaagaac
     4801 gccgagccct tgatcgtcgt gacggtctgc ttgccggcgt tgatgaggtc cggatcttcg
     4861 tcgccttcgt acgggaacgg gccgatgccg agcaggccgt tctcggactg cagcacgacg
     4921 tcgatgccgg gcgggatgaa gttcgcgacg agcgtcggca tgccgatgcc caggttgacg
     4981 tagaagccgt cgcgcagctc ggcagcgacg cggcgggcca tttgatcacg atcgagacgc
     5041 ggcattacgc gctcctcttg cgcagcgtgc gctgctcgat gggtttctcg tagtgggtgc
     5101 cctggaagat tcgctgcacg tagatcgacg gcaggtgcac gtgatcggga tcgagctcgc
     5161 cgacttcgac gagctgctcg acctcgacga tcgtgatctt gccggcctgt gcgcacagcg
     5221 gattgaagtt gcggctcgtc ttgcggaaca cgaggttccc ccacttgtcg cccttccagg
     5281 ctttgacgat cgcgaagtcg ccgcgaatcg cgtgctccat cacgcactgc ttgccgttga
     5341 tcgtgcgcgt ttccttgccc tcggcgacct tcgtgccgaa gccggtcggc gtgaagaatg
     5401 cctcgatgcc tgcgccgcct gcgcgcaggc gctcggcgag cgtgccttgc gggcagagct
     5461 cgacctgcag ctggccggac aggaactgct tctcgaagat cttgttctcg ccgacgtagc
     5521 tcgagatcat ctttttcacc tggccctgcg cgagcaggac gccgaggcct ttctcggtcg
     5581 tgccgcagtt gttggacacg atggtgagat ccttgacctg tcgccgcgct agctcggcga
     5641 tgcagttctc ggggttgccc gacaggccga agccgccggc caggatgacg gcaccgtcga
     5701 acacgccttc gagggccgcc tgggcgtctg ggtagacctt gtgcatcgtc tgtgtcttac
     5761 tgccaaaggg gatgccgatc atcacgttga cgacggattt cggcacgcgc gacgggtacg
     5821 tcggtgcggt caagggcgtg atccatcgcg tgctccagag cacggtcgtg cgcccggtct
     5881 gggagcacac gagcgtcgtc gacattgctc acgatctgcc gcgcggcgac atcgcgcacg
     5941 ccgcgtgggt cctcgcgaca tcgacgatcg aattcccgga agggtcgatt cacgtcattg
     6001 tcgtcgatcc cggcgtcggg ggcgcgcgcc agaacgtcgt cgtcgaatcc gcgggcggcc
     6061 agctctacgt cgggccggat aacggcgtgt tcgcgttgct cggcgacacc agtgcgcacg
     6121 tggtcacgaa gaccgcgctg ctcgcgccga agatcagctc gacgtttcac ggccgcgacc
     6181 tcttcgcgca cgtcgcagcc cgcctcgcgt gcgggtatcg accgacggat ttcggcaagg
     6241 cgacgcggct cgtcggcacg ctgccgtggg ggcggcgtcc ggagggcgtg ggtcgcatca
     6301 tccatatcga cggcttcggc aatctgatct cggatctgcc ggcgcgcgag gcgggtggga
     6361 ccgtgctgtt cgagacccgc cacattccag tgatgcgcac gtatgaagac gtaccggtcg
     6421 gcgagctgct cgcgtacgtg ggatcggcgg ggaccatcga gattgccgtg cgcgacgggc
     6481 gcgcagacgc cttgctcggt gtgcagcgcg gcgccaaagt gctaccggat ccggacgcga
     6541 ccggaccgta tcgatgagtc cctcgtcagg gatcaccatg ggcgctgaaa tcgaagtcga
     6601 aatcacgtcg ctcgccgcgg gcggcgatgg tgtggcgcgc gatgcaaatg gccgcgtcac
     6661 gttcgtgccg cgcagtacac cgggcgatgt cgtgcgcgtg acgctcacga agcaaacgaa
     6721 gtcgttcgcg cggggaacca tcgtcgagat cgtgcggcgg tctccacagc gcatcaaccc
     6781 gccatgcccg tacttcgttg ccggctgcgg cggctgccag tggcagcacg tcgagcgggc
     6841 agggcagctc gctgccaaac acgcgatcgt gacgaacgcg ctgcgccacc tcccgggcgt
     6901 cgtcgtcgaa gacgtgctcg atccggctcc agcgtatggc tggcgacgcc gtgcgcgctt
     6961 tcatggcgcg cacggcttgg tcggcctgta cgagcacggc agccatacgc tgatccgcct
     7021 gcaacagtgc ccgcagctcg agccggagct cgagccgata ttgaaggagc tcaatgcgct
     7081 gcggccgccc gagggcgagg tcgcgctgct ccgcggtcac accggtgaca tcgtgctcgg
     7141 cgtcgagcac gcctggcgcg gtgccgcgaa gctcgtcggc cgcgtcggca tcgtcggtgt
     7201 cgtcgccgga aacgagacgc acggcacgac ggtgatcgag atcgagcccg gcctgttcgg
     7261 cgggccgtgg gacttcgcgc aggcgagcag ggccggcaat gcgaagctca tcgaggtggc
     7321 gcgtgctgcg gtcggcgcgg cgggtggtgt gaattcgtcg acgagcaagc ggttgctcga
     7381 gctgcatgcc ggcgcgggca acttcacgcg gacgttcgtc gccgacggct gggacgtgct
     7441 cgcgagcgat gtccacgcgc ccgcgcgacc gattgctggc gcgcgcttcg agggtggcac
     7501 cgccgaggat gtgctcgcgc gcgccgccgg ccccttcgac gcgatcgtgc tcgacccacc
     7561 gcgcaccggc gcagtcgagg cgatgccggg tatcgcgaag cacgcaccac ccgtcgtcgt
     7621 gtacatctcg tgcgatccgg cgacgctcgc gcgcgacgcc gcgaagcttc tcgaagttgg
     7681 ttaccgcgcc gagcgcgcat ggccggtcga tctgatgcca cagacctcac acgtcgaggt
     7741 cgtgctccga ctgacgaagg gctaaccggc cttcttcgtc ggcgccggtt caggcttcgg
     7801 ttctttcggc ggcgcgaacg tcttcacgac cacgcgtgcg gcggcgacgt cgacgccctt
     7861 cggcacttcg attgcggcag acaccgagta ggtgcgaaac agcagcacgc ggacgacgat
     7921 cgtcgagcca ccgtcccgct tcgtctcgag atcgagcgcg gggacaccgt tcgcgtcgac
     7981 gattttcttg gcgcgacgct tgtagccagg gacgcccgcc ttgatgccgg actcgacctt
     8041 gtcgacgtac gcctgcttct tgtcactgat ccatgcgtcg ggattcggca catccgcgcg
     8101 cgtgacgacg agcgtcgcgc cgttgtcgtg cttgaagatc tcgacgacgc ccttgaacga
     8161 gtgcttcacc cggttccagc catcgggcaa ggtcagtgtg gctttcgaat tcggcagcga
     8221 gacgatcgtt tccgcgtgcg ccactccacc gagcagcgcg agtacgacga acagccggat
     8281 cacgactcgg tgaccgggcc ggcgtcgctc ttcgcgtcgg acttcgtgtc gctcttcgcg
     8341 tcggacttcg cgtcgctctt gccgtccgcc ttgtcggccg gggcgtcgcc acgcggacgc
     8401 aggccgagca gggcgtggat cacgtggcgc tgatcggctt ccgacgcgaa ccagtcgcgc
     8461 gggaatgcca tcgccttgtc gccaagctcc accaccagca ccggcgccga gcgattccac
     8521 ggcacggagc ggcgcaggaa gtacgctgcc gtcacagccg acggctcgag cacgagcggc
     8581 cgcgcccggt gcacgccgcg cggcaggctg accttgtctt ccgagatcac gaacttgccg
     8641 ggcggatgca gaaagctcat cgcgagctgg tagccgcgga acaggccgag cgcgatcagc
     8701 acgtagccga cccacacgcc gaccgtaccg agcttccaga tcagcagtgc gccgccgatg
     8761 attccgccga tggtccagct gatccagcgc tggcgcgata cctcgagctc gacctcgacc
     8821 gtctcgagcg gagcccgcct cggatcctcg tgacgatacg aggtctcctt ggcgtcctta
     8881 gcgcgaccag tctcgggtga gctcgctgac gcgtgcttgg atctcatcga gggagacatg
     8941 gcagagacgt cgtgccgtaa cgacctcctg accatctacc acgacgtcgg tgatggcgcg
     9001 aggcgagagc gcgtagacca cgttcttttc cagggcttgg ctcggccaga gggacggatc
     9061 gtcgagatcg agtgcgacga ggtcgcagcg ataccccggg gtgatccggc cgatcgggag
     9121 ccgtagtacc tcgcctgcgg tacgcgtacc gagcgcaaag caggtctcgg cggcgatggc
     9181 ttgcccgtcg gtcttgtgga ccttctggag cagggcggcc atccgcatct cgtcgaagac
     9241 gctgacccgg ttgttcgagc agccgccgtc ggtgcccagg cccacccgca cgccgcgggc
     9301 caccagatcg acgagatcgg tgacgccatc gccgaggaac atgttcgagc ccgggcaata
     9361 ggcgagcgag ccgccgcgct cggcgagcag ggaccgctcg gattcgtcga accagcagcc
     9421 gtgcaccgcg atcatcccgg acatgtcgac ctggaggccg gcgaccgcgt ggatcgggcg
     9481 cttgccgaac cgctcgagcg actggtcgac ctggtacttc tcctcggcga ggtggatgtg
     9541 ccatggctcg ccggcgtcgg cggcgcagcc ggcaccggct tcgatcatcg ccggcgtcgc
     9601 gccgtgctgg ctgtgcggcg cgggctggat cgtgacgagg tggcgaacct tgtcgcggta
     9661 gcgcgcgtgc agcgcctggt agttcgcgac cgcctgcggg atcgattcac gatacgccgc
     9721 gggcgcgcca tcccagtcgt agaaacagcg cgcgagcacg atgcgcatcc cgagccggcg
     9781 cgcggcctcg atcgttgccg acgcgtggtc gttgccctgc gcgttcagat agtagaagtc
     9841 gcagacggtc gtgacgccgt ggagcaacat ctcgccgaac gcgaacagcg cggcggtacc
     9901 catgtcgtcg gcggtgaggc gtggcgagta gcgatacagc gcgcggtcgc gccactcgag
     9961 aaatggcagg tcgtcgccga tgccgcgcaa cagcgattgg aagctatggt tgtgcgtgtt
    10021 gaccgtgccg ggtacgaccg cgcgatgtga ccagtcgagc cgccgcgcac ctgccgcgcg
    10081 tgggtcggcg gccacatcgc cgggatcacc gacgagcacg atctcgccgt cgatcgcgag
    10141 gatcgccgtc ggaccgcgcg cgccgccgcc ccgtgtcacc gatccgagcg cgccggcgaa
    10201 gccggcgggc tcggtgatcg cgcgatccgc agtaacgagt agcgtgttcg tcatcggcgc
    10261 agcgacccta tcattggtgc atgctagggt ctgccccgtg caggagttga tcgagaaagg
    10321 cctcgccccg atctacgtgt tccactcgga acacccgatc ctgatcgaac gggcggttgc
    10381 cacgatccgc gacgctgccg tgccgccggc gtcgcgcggc ttcaactacg acgtcatcga
    10441 gggcaaaccc aagggcaacc agatcgtcgc gctcgcgcag acgctgccga tgatggcgaa
    10501 gcaccgcatg gtgttcgtgc gcgacctcgg cctcttgccc gccgacgagg ccgagccggt
    10561 catcaattac ctgagcaagc caaacccgtc gacggtgctc gtcgcgatca cgagcaagct
    10621 cgacaagcgc ctcaagctgt tcgcgcagct gtcaaagaag ggcttcttgc acgtgctcga
    10681 ggcgccgcgt caggtcgggc cgtgggtgcg cgaggaagcc aagctcaagg gcgtgcgcat
    10741 cgacagcgct gcgatgtcgc ggctcgtcga caccgtcggc aatgacctgt cgcggctatc
    10801 gctcgcgatc gagcagcttg gcctctacgc cggcgatcgt ccggtcacca gcgacgacgt
    10861 cgacgagctg atcgccgaca cgcgcgagcg ctcggtgttc gagctcaccg acgcgatcgg
    10921 cgccgcggat cgcgggcgag cactcgccgc ggtcgcatcg ctgtgtgatc agcgcgagag
    10981 cgcggtcggt gtcgtcgtca tgctcgcgcg tcatatccgc cagctgtcga tgctgcacac
    11041 ctgccgcatg cacggcgtgc cgcggtccga gtggaacgtg cggatcggcg tgccgccatt
    11101 cgtcgtcgac aaactcgtcg cacaagcgaa gagctacacg cccggcgcgc tcgccacggc
    11161 gacccagcgg ctcgcgaacg cggaccgcgc gctgaagggc gacatcacgc tgaccacaca
    11221 gacggcagca ttcacaggcc ctcagct
//
LOCUS       DASUFX010000375        20810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1590026, whole genome shotgun sequence.
ACCESSION   DASUFX010000375 DASUFX010000000
VERSION     DASUFX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20810
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590026"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFV99_14995"
     CDS             complement(<1..218)
                     /locus_tag="VFV99_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase/pyrazinamidase"
                     /protein_id="HEX5060673.1"
                     /translation="MRALLLIDLQYDFCPGGALAVANGHETIPVANRLMPKFSTIVAT
                     QDWHPAHHGSFAVNHPGKKPYDMHELAG"
     gene            complement(228..917)
                     /locus_tag="VFV99_15000"
     CDS             complement(228..917)
                     /locus_tag="VFV99_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5060674.1"
                     /translation="MSFSYEYARPALAVDCVVFGLDENDLKVLLIQRKLQPFQHAWAL
                     PGGFVRIEETLDDAARRELEEEAGVSDVFLEQLYTFGTLDRDPRDRVVSVAYYALAKL
                     SDHRIRAATDAMGVGWFGLDDLPKLAFDHSEIVARAHERLRGKVRYAPIGFELLPPRF
                     SLTQLQRLYEIILGTGLDKRNFRKKILAMDLLVETDEVEQGVRHRAARLYRFDRRKYE
                     RLARQGFEFAM"
     gene            complement(914..2941)
                     /gene="nadE"
                     /locus_tag="VFV99_15005"
     CDS             complement(914..2941)
                     /gene="nadE"
                     /locus_tag="VFV99_15005"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX5060675.1"
                     /translation="MKLIKVAVACVNQTPFAWETNFAHLRMAIEHARAEGATMLCLPE
                     LAITGYACEDAFFMDGLQDVAFSQLDALAPLTRGMVVAVGMPVYHEKALYNAAALLAD
                     GKIVGFVGKQFLAADGIHYEPRWFKPWPAGEVDVLERTAEDGSIRRYPIGDLVFDIGD
                     VRIGFEICEDAWVANRPGTDLALRGVDIMCNPSASHFAFGKFAVRQRFVVEGSRAFGV
                     AYLYANLLGNEAGRVVYDGGGLIASGGELLVRGRRFSFHDVELATAIIDVDGNRREQA
                     RRGSHRPRHDAEQQVVEHPFVWPQRKPEAPRPRLHSWEDRHSLKEEEFARCVALGLWD
                     YLRKSRAQGYVVSLSGGADSAACAVLVALAVQLSFQELGAGGVRQHLPWCRRLIETLD
                     KGGGATAAVGALLTCAYQPTENSGKVTRIAAETVAKAVGAEFHVIDVDAQYKAYITTL
                     EKAIGRSLSWDRDDLTLQNIQARVRSPSIWMLANVRGAVLLTTSNRSEAAVGYATMDG
                     DTSGGLAPLGGIDKSFLRAWLRWMEKKGLVGLEPIPALGVINSQQPTAELRPPTSSQT
                     DEADLMPYDFLEAVEDSAIRDKHTPTEVLQELLPRYPEHSPAQLAMWIERFFRLWCQN
                     QWKRERIAPSFHLDDRNVDPRSWCRFPILSGGFERELAEMRAYVAMGKAER"
     gene            complement(2949..3869)
                     /gene="prs"
                     /locus_tag="VFV99_15010"
     CDS             complement(2949..3869)
                     /gene="prs"
                     /locus_tag="VFV99_15010"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX5060676.1"
                     /translation="MHEPLVFSTAAYTYLADAIAKEGGWERGVITRKTFPDGEHYRRI
                     DTDPADRDVIFVGGTIDDEAAVELYDLACGLVMFGAYRMRMVIPYYGYSTMERSAKAG
                     EIVTAKTRARLISSIPMASRGTQVFLLDLHVGGLAHYFEGGIRPIHVYGKKLTTAAAR
                     RLGGADFVLASTDAGRAKWVESLAKDLQVDAAFVYKRRTSGTETEITGVSANVAGRRV
                     VIYDDMIRTGGSLCAAAEVYKDAGAVAIDAIATHGVFPRDSLAKIRNRGLLGRVVTTD
                     SHPRAVSLASEFLEVDSTAALLTEHLKSNR"
     gene            complement(3862..5331)
                     /locus_tag="VFV99_15015"
     CDS             complement(3862..5331)
                     /locus_tag="VFV99_15015"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX5060677.1"
                     /translation="MTDGLDDVYGGSLALLTDLYQLTMAAGYWKSGHAEREAVFHLTY
                     RRPPFGGGYAIAAGIAPVVAYLKRFRFMPDDIAFLATLKDNEQKPLFPPAFLGYLHEM
                     KFSCSVDAVPEGALVFPHEPIIRVRGPIVQAQLIETPLLTLVNYQTLIATKAARLAQV
                     AKGPVLEFGLRRAQGIDGGLGGSRAAYIGGCAATSNTLAGKLLGIPVKGTHAHSWVMF
                     HGDELSSFRTYAETLPGNCTFLVDTYDTLDGVRNAITVGKELRARGHELAGIRLDSGD
                     LAHLSAEARAMLDAAGFPNAKIVASNDLDEHLIQNIQDQGARIDIWGVGTKLITAFDQ
                     PALGGVYKLGAYRDDSGAWREAIKLSEQPIKISNPGMLQVRRLRNGNEYVGDVIFDSD
                     HGCETPCELHDIEDPLRGPVAPVHDHAEDLLTTVIDRGRVLPLPDLEAARARAAHDLA
                     MLSPRTKRFLNPQPYPVGLDGHVHRRKQQLIAEARARHA"
     gene            5368..6318
                     /locus_tag="VFV99_15020"
     CDS             5368..6318
                     /locus_tag="VFV99_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5060678.1"
                     /translation="MSNGSDQRAPAAKPGAEAVISDQAVIVWGEVLWDRFPDGDKLGG
                     APSNVAWHLGLAGGWARLVSRVGDDAAGREAIERLSNVCDADLIQIDSERPTGEVRVA
                     IENGEPRYTLVPGCAWERIECTADVAAALGEAGVMVYGTLAQHTETGIAGWRAAIAAA
                     HGSCLKVCDVNLRRLSIAPPVEQRAVKEAIAAADIIKVNDQELTRIADWFGWLDPLAE
                     LRAGRRVVAVTHGKNGATLYGDKEIVTIAGVQSKPGGDNVGCGDAFVALFVHGMTLGW
                     DLRTSGTAACRWAAAVAGVRGATPMFDEEQIAELLGEAAS"
     gene            6369..8717
                     /locus_tag="VFV99_15025"
     CDS             6369..8717
                     /locus_tag="VFV99_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060679.1"
                     /translation="MGTTTESRQAARVENTEDKPSAPVIKKRESFFARHLGRYFGGST
                     DKKPGGESPDSWDQVAAWFLTNGALPALALITVAVFGIHAHIFRGETAGDDLSFHFAE
                     SARLMDCIRHGDFDFWNPSANAGYASAYYYQVLPQLASALPAAIFGHHLFWFQLSVVL
                     PLVLAPAAAYKGMRLLGATPWQSVVGAFCVGFMNGASRWGAGNAGTFQVGLYTQTWAL
                     CAFPMALGYAYLWMTKGKRLASTIAWSAFVTLCHPFAGITLGFAALAAWFATFVLLPF
                     DYLFAALAPKLTGRLTWLGKRWAEPPRRAKEILPELVRGGLFFIGLVIAVMPVWLPLV
                     IDPQGFGGFPHRVGDEVGPGFKTLAHWYANGEILDWLAPSAGLGTRLGLLTWSLPLVV
                     ILARAPYYRWLWAPAVLYALLLGVGPNIGKIGDDLIPPVRFLGGMQTVLAMGIGAGVV
                     LIGQRAWEIVGRWPSKAYALRTVIAAIAAAAIVLVVVPGGRALGARVRVLGDHSNSHA
                     DELFLVNAILARQPQGRKQTGPGAENHWWNLLSYVYERVPSTLQMGGGGLQASPNYDF
                     LWTQRDLVKNAWIFDAPYLVYMSSLGSKMPIAEMIAKTPNYEVRRLPSAGLVSPIQVV
                     GVLPPGYSHKQEGHKKALEWIKSDQPMKDEMLAYVGSEHGNSDAPAGRTLRSWRQDSP
                     GSDPDIVAEVEAEKTTTFVVRESWHPRWHAYIDEDQEVPVRRVTPDFIAVDVPPGKHT
                     LAVRFERPWWAHASWLAWPLTVLLAFFAMRWWSRRNATSPVA"
     gene            complement(8703..10274)
                     /locus_tag="VFV99_15030"
     CDS             complement(8703..10274)
                     /locus_tag="VFV99_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060680.1"
                     /translation="MAGAPDDRDKELPELPAASSDDPSSAKTDMPASASGSVDVKVSQ
                     PELPAGASGSVDVKLSQPELPASASGSVDVALSHPGIPQAVPAADSKPVASGGVVAVA
                     TAHDTGPVGGRSKRITKAMQDAASGAVERLGASIGTIGEGVAKVGDVTKKVPLVGASV
                     GKLGEGLTKAGESIHALPRVAQTRRGRLLVRSVIVGFLIVFSWIALIVFIQLRSATTP
                     DFRPQAEKILMEISSGKQGIEDVYEKASPRFQEFVLKERFVDLMKDLDATNGKFQEIT
                     AINETVVGTGPTGPVARVGLTALYEHGITKGSISFHKDKDGKWKFLGIGIEVPASVKI
                     TQAEREKRVAACLDEKGHDVSDQPADHAHGKPAKCDVRDAAETILEMIRDGKAGEVYD
                     SASDIFKQQETRANFIRIYEDNKIPLGDYKRLLTVTEAKSIGGLTAFFDTVAEFEKSS
                     GVRIIFNFSRPSKTAKWQLRSLKVVVPMPRPDEERSLVPPAPDVQVDEPPLMREMGSP
                     PPPSRPDAAVGSGHR"
     gene            complement(10277..11113)
                     /locus_tag="VFV99_15035"
     CDS             complement(10277..11113)
                     /locus_tag="VFV99_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5060681.1"
                     /translation="MTGYARPRDLKEALDLRAKHPDWMLLAGGTDLMVNANHRPAPAG
                     ILDLWRLPGLGGIVAADSHVTIGAGATWYEVETHTAILERWLPLALAAREIGALQIQA
                     RGTLGGNVGNSSPVGDSLPVLLALDAELELASVRGTRRIAYKDFCTGYKKTALAPDEL
                     IVGATLPAPSKTTRTTWRKVGTRRAQSISKVMGAAAIDLDGGVVKSARVALGAVADRP
                     IRVAAVENAVRGLTPDQAAEAARAALRTAIKPIDDVRSTAVYRLAVAENLVARFFSGI
                     LS"
     gene            complement(11110..11598)
                     /locus_tag="VFV99_15040"
     CDS             complement(11110..11598)
                     /locus_tag="VFV99_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5060682.1"
                     /translation="MSMKITLEVNGEKRTVECSPWKRLLDVLREDVHLTGTKEGCGEG
                     ECGACSVMLDGEVVNSCLVAAGQVDGRRVTTVEGLARGDVLHAVQKQLVTCGGAQCGI
                     CTPGIAVCATHVVDRGLIRGKDEAAARAAVRELLAGNICRCTGYQLIVDAVIAAAREV
                     ES"
     gene            complement(11600..13741)
                     /locus_tag="VFV99_15045"
     CDS             complement(11600..13741)
                     /locus_tag="VFV99_15045"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5060683.1"
                     /translation="MDGAAKVTGRARYLDDLQAEGAWFGATVRSKIAHGVLEAIDLDP
                     AFDWSQVTVVTAADIPGSNYIALIENDQVALVPIGGRVMHVDEALALVAAPTKELAFA
                     AAKAVKPRVKPLPGVFTIDDSLALTQKLYGDDNVFKQFLIRKGHDDDAAFDAAFARAA
                     QVIEGTYWTSAQEQMYIEPQAMMARWDGGRCYIVGSMQCPYYVHKAMKPLLGCGPDDV
                     VISQSVTGGGFGGKEEYPSMLAAHVAVLAKKAGRAVKIVYDRDEDIAATTKRHPARTH
                     HRIAVDERGEIIAMDVDVVMDGGAYLTLSPVVLSRGVLHAAGPYRCPVTRVRGRVVAT
                     NHPPHGAFRGFGAPQTTFAYERQIQKLAIARGEDPMALRKRLALRSGDTTSTGQLLGF
                     SVGTDEVIGAIERVAGPAPRKDGVRNGAPVRRGRGVGFYFHGAGFTGSGEQRLAGRAT
                     VAKTVDGRYEVRSSSTDIGQGAITIFTQIAAGALGIDPSQVEVVDPSTAQVPDSGPTV
                     ASRTCMVVGGLVEKAARALKEKLKEPHDGEVAVTMQYEPPPGIHFDDATYTGAAYPCY
                     GWAACLVDVSVDLDTYEVHVDRCVQAVDVGKAINPVIVKGQIEGGTLQALGWALLENV
                     VYKDGKVMNANMTNCIVPTFADAPELETILIEVPYPFGPSGAKGVGEIPMDGPAAAVA
                     NAVENALGCEFDAIPIMPEVVAASRPRWRLG"
     gene            13932..14900
                     /locus_tag="VFV99_15050"
     CDS             13932..14900
                     /locus_tag="VFV99_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5060684.1"
                     /translation="MKRGLDASDVRRAVDHVIESGLDFFDVAAEESAESLVADAIRSL
                     RVRDRVIAAYRIPAIAQRLGIPTRDTLPERLPPRYIVDRVESILRATRLDAIPLAQLT
                     LRALWRSSSAWPEVTGTCARLVREGKVLDWAAFVDAIEDDTVELTHEPWLAAISVPYS
                     LCERAADPILDAAALVSAAPQTTAAAERKLTILARRPLAGAALAGSLGPGAKLRMHDD
                     RRSIDDATLERIAATVAKLARFTKQTPPAAFSCDAAKAQLDRNVRPEHLHVQTLAELA
                     LRFVVTRGAIALPRLHRKEHVADALIAAHSPPLPRDLIESIEKLDI"
     gene            14926..15957
                     /locus_tag="VFV99_15055"
     CDS             14926..15957
                     /locus_tag="VFV99_15055"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5060685.1"
                     /translation="MKARPTENPPIRFRDVTVAYDEGDGPAPATITLLEDQSRSTLSH
                     NDSPDLGFNWSANPYRGCQSACHYCYARPTHEYLDLGAGTDFDTKIVIKPRAGELLRA
                     AFDKPSWKGELIMFSGVTDCYQPIERDLQLTKQCLEVCLEYRNPVAIISKHALVERDV
                     DLIAELAREASAHLSVSLAWIDAELARTIEPWAASPQRRLKVIETFAKAGVPVGVMCA
                     PIIPGLNDNQIVKLLEAAKDAGAQWAGWTLLRLPGVVKEVFEDRLRVSLPLVADKVMH
                     RVRETRGGEKLYDSQFHTRGRGVGVYAETIKALFETTVKRLGLNERDWGDRPSRFQRP
                     NRSPQLSLF"
     gene            complement(16163..16393)
                     /locus_tag="VFV99_15060"
     CDS             complement(16163..16393)
                     /locus_tag="VFV99_15060"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX5060686.1"
                     /translation="MRKEYDFSKARKNPYARQLKRAVTIRIDRDTLEYFKELAAKVGL
                     PYQTLINMYLRECAMEKKELRLHWLPKRKSEK"
     gene            complement(16393..16680)
                     /locus_tag="VFV99_15065"
     CDS             complement(16393..16680)
                     /locus_tag="VFV99_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX5060687.1"
                     /translation="MDDEIRFEWDPAKNAHNMARHGVSFEEAALVFIDEYALLIDDPD
                     HSDVEDRFILLGRSLYGILVVCHCYRADAIRIISARKASARERRAYLEGLN"
     gene            complement(16871..17848)
                     /locus_tag="VFV99_15070"
     CDS             complement(16871..17848)
                     /locus_tag="VFV99_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060688.1"
                     /translation="MRRLLCCVVVVVLAGLVEARGAWAADQETPVELQMSSIDQRQLT
                     KRLDDGDTLTGKAQIKLRYYRETTWLLEDEVDVGTGAKVEIAKLDRKVKLDRSKQVEC
                     HVWLPTTKGKFLGVVGVSKPSQVLGSKPSAFKLGCRLMGAALAKQDAVDAKWPDIGTQ
                     TDVNFWYSVNIDAAIGNAVAKAKDDVSRANGKLAVLVELFDPATKEKLGVRSFELTPR
                     KPKGLEAFAETTNADSGPTEIKLPEGQPVLFRLRMDTQKGGVVFAEGEVTAWRLGSNQ
                     PRSTASIEAKAFKFEKDPKKAPKPGEWGATKTPAKAVPAKAPAKAPAKK"
     gene            complement(17901..18731)
                     /locus_tag="VFV99_15075"
     CDS             complement(17901..18731)
                     /locus_tag="VFV99_15075"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5060689.1"
                     /translation="MLRVEVSEAGQAALPAVDVDDAVVVIGSATNARIRLPANAAERE
                     HVRVEGKRWRAVGAVKVDGAPRDGGDIGDGVTLEVAGYRVRLMPAPAGAQAAPVQRTE
                     SLARELMRNLLGSAGQPTLEIERGARAGAKRALQPPESTLVIGRGDEANWIIDDKDLS
                     RTHAEIRRGWDGTRIVDLESKNGTKVDGDPVGAGGMALRDGCLVELGPVVMRFRDPAE
                     KHLQGDAPAFRPLLADAPPLKPAPAPSVAVPRGNPVIFYGALAIMALALAGLVWILAS
                     "
     gene            complement(18741..19382)
                     /locus_tag="VFV99_15080"
     CDS             complement(18741..19382)
                     /locus_tag="VFV99_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5060690.1"
                     /translation="MLVAMMLVAGLAVAAHADVYSWTDEDGVVHFTNIKPSGGKWKKV
                     LDSQPPKGSKASAERCPRCDKVGATDKSPERFHRYDAFIQEASDLYRIPVPLIRAVIK
                     VESDYDPKVVSAMDCKGLMQVHPDVEVDMGVQGDVFDPRTNIMAGTRLLRWLANRLDG
                     DLVLTIAGYHAGLGSLQKFGYTVPPYAYTRQYLKMVLERYYQYKAEEAKQQTK"
     gene            19448..>20810
                     /gene="nadB"
                     /locus_tag="VFV99_15085"
     CDS             19448..>20810
                     /gene="nadB"
                     /locus_tag="VFV99_15085"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5060691.1"
                     /translation="MAEPLETDYLVIGSGIAGFNFALLAAEHGRVVVCTKKRPNDTNT
                     NWAQGGVAAVLAPDDSFEQHVSDTLNAGDGLCDRKIVELCVNEGPAQVKRLLDIGVRL
                     ARDSAGQLDLGREGAHTHHRVVHYEDVTGREIQRALIAAVQSHPNITVLDSHIAIDLL
                     SMAKYGGDPACFGAYVLDSRSGEVKTICARATVLATGGTGKVYIYTSNPDVATGDGVA
                     MAYRIGAACGDLEFVQFHPTVLYHPMAGSFLISEALRGEGGVLKLANGETFMEKYHPM
                     KSLASRDVVARAIDNELKKSGADAVYLDMTHLDPGFVRGRFPNIYERCLSLGIDITKQ
                     PIPVVPAAHYMCGGVKTDENGATTIRGLYAIGECAFTGLHGANRLASNSLLEGMVFST
                     RAASAVRDAPRIRPSQVAPWSSGNATDSNDAIVVSLNWEEIRRFMWSYVGIVRSDKRI
                     MRAH"
BASE COUNT         3433 a         7065 c         6721 g         3591 t
ORIGIN      
        1 aagccggcga gctcgtgcat gtcgtacggc ttcttgccgg gatgattcac cgcgaagcta
       61 ccgtgatgcg ctggatgcca atcttgcgtg gcgacgatcg tcgagaactt cggcatgagc
      121 cggttcgcga ccggaatggt ctcgtggccg ttcgcgaccg cgagcgcacc gccagggcag
      181 aagtcgtact gcaggtcgat gaggagcagc gctcgcatgt cggaagtcta catcgcgaac
      241 tcgaacccct ggcgcgcgag ccgctcgtac ttgcggcggt cgaagcgata gaggcgcgcc
      301 gcgcggtggc ggacgccctg ctcgacttcg tcggtctcga cgagcaaatc catcgcgagg
      361 atcttcttgc ggaagttccg cttgtcgagg cccgtgccga ggatgatctc gtacagacgc
      421 tgcagctggg taagcgagaa gcgcggcggc aacagctcga agccgatcgg tgcgtaacgc
      481 accttgccgc gcagacgctc gtgagcgcgc gcgacgattt cgctgtgatc gaacgcgagc
      541 ttaggcagat cgtcgagccc gaaccaaccg acacccatcg cgtcggtcgc ggcgcggatg
      601 cggtgatcgc tcagcttggc gagcgcgtag tacgcgaccg agacgacgcg gtcgcgcgga
      661 tcgcgatcga gcgtaccgaa cgtgtagagc tgctcgagaa acacgtcgct gacgccggcc
      721 tcttcttcga gctcgcggcg cgctgcgtcg tcgagcgtct cctcgatccg cacgaagccg
      781 ccgggcagcg cccacgcatg ctggaacggc tgcagcttgc gctggatcag cagcaccttg
      841 aggtcgttct cgtcgagacc gaacacgacg cagtcgactg cgagcgcagg cctcgcgtac
      901 tcgtaggaaa agctcaccgc tcggccttcc ccatcgcgac gtacgcgcgc atctccgcga
      961 gctcgcgctc gaagccgccg gacaagatcg ggaagcgaca ccaggagcgc ggatcgacgt
     1021 tgcgatcatc gagatggaag ctcggcgcaa tgcgctcgcg cttccactga ttctggcacc
     1081 acaggcggaa gaatcgctcg atccacatcg cgagctgtgc gggcgagtgc tcggggtaac
     1141 gcggcaacag ctcctgcagc acctcggtcg gcgtgtgctt gtcgcggatc gccgagtcct
     1201 cgacggcctc gagaaagtca tatggcatca ggtccgcttc gtcggtctgc gacgacgtcg
     1261 gcgggcgcag ctccgcggtc ggttgctgcg agttgatcac gcccagcgcc ggaatcggct
     1321 cgagcccgac gagacccttc ttctccatcc agcgcagcca cgcgcgcaag aacgacttgt
     1381 cgatgccgcc gagcggggcg agcccgccgg acgtatcgcc gtccatcgtc gcgtagccga
     1441 ccgcagcttc ggagcggttc gacgtcgtga gcagcacggc gccgcgcacg ttcgcgagca
     1501 tccagatcga cggcgaacgc acgcgcgcct ggatgttctg cagcgtcagg tcgtcacggt
     1561 cccacgacag cgaacggccg atcgccttct cgagcgtggt gatgtacgcc ttgtactggg
     1621 cgtcgacatc gatgacgtgg aactctgcac cgaccgcttt cgcgacggtc tcggctgcga
     1681 tgcgcgtgac cttgcccgag ttctccgtcg gctgatacgc gcacgtgagc agcgcaccga
     1741 cggccgcagt agcgccgccg cccttgtcga gcgtctcgat caaccgccgg caccacggca
     1801 gatgctggcg cacgccgccg gcgccaagct cctggaacga gagctgcacg gcaagcgcga
     1861 cgagcaccgc acacgccgct gagtcggcgc cacccgagag cgacacgaca tagccttgcg
     1921 cgcgggactt tcgcaggtag tcccagagcc cgagcgcgac gcagcgcgcg aactcttctt
     1981 ccttgagcga gtgccgatct tcccagctgt gcagacgcgg acgcggcgct tccggcttgc
     2041 gctgcggcca cacgaacggg tgctcgacga cttgctgctc ggcatcatga cgcggccggt
     2101 ggctgccgcg acgcgcttgt tcgcggcgat tgccgtcgac gtcgatgatc gccgtcgcga
     2161 gctcgacgtc gtgaaacgaa aacctgcggc cacgcacgag aagctcgccg cccgaggcga
     2221 tcaggccacc gccgtcatag acgacgcgac cggcttcgtt gccgaggaga ttcgcgtaga
     2281 ggtacgcgac gccgaatgca cgcgagcctt cgacgacgaa gcgctggcgc acggcgaact
     2341 tgccgaatgc gaagtggctc gcgctcgggt tgcacatgat gtcgacgccg cgcagtgcga
     2401 gatcggtgcc cggacgattc gcgacccacg cgtcctcgca gatctcgaag ccaatgcgca
     2461 cgtcgccgat gtcgaatacg agatcgccga tcggatagcg gcggatcgat ccgtcttcgg
     2521 cggtgcgctc gagcacgtcg acctcaccgg ccggccacgg cttgaaccag cgcggctcgt
     2581 agtgaatgcc gtcggcggcg agaaactgct tgccgacgaa gccgacgatc ttgccgtcgg
     2641 cgagtaacgc agctgcgttg tacagcgcct tctcgtgata gacgggcatg ccgaccgcga
     2701 ccaccatgcc gcgcgtcagt ggcgcgagcg cgtcgagctg cgagaacgcg acgtcctgca
     2761 gaccgtccat gaagaacgcg tcctcgcagg cgtagccggt gatcgcgagc tccggcagac
     2821 acagcatcgt cgcgccctcg gcacgcgcgt gctcgatggc catgcgcagg tgcgcgaagt
     2881 tggtttccca cgcaaacggc gtttggttca cacacgcgac ggcgaccttg atgagcttca
     2941 tgggagacct accggttgga tttcagatgc tcggtgagca gggctgcggt gctgtcgact
     3001 tccagaaact ccgaggcaag cgagacggcc cgcggatggc tatcggtcgt gacgacgcgg
     3061 ccgagcaggc cacgattacg aattttcgct agcgaatcac gtggaaacac accgtgcgtc
     3121 gcgatcgcgt cgatcgcgac ggcccccgca tccttgtata cctctgcggc cgcgcagagc
     3181 gacccgcccg ttcggatcat gtcgtcgtag atgacgaccc ggcgacctgc gacgttggcc
     3241 gacacgccgg tgatctcggt ctcggtgccc gacgtgcggc gcttgtagac gaacgcggca
     3301 tcgacctgga gatccttcgc aagcgactcg acccacttcg cgcggccggc gtcggtggac
     3361 gcgagtacga agtctgcgcc gccgagccgc cgcgccgcag cggttgtcag cttcttgccg
     3421 tagacgtgga tcggccggat gccgccttcg aagtagtgcg cgaggccacc gacatggagg
     3481 tcgagcagaa acacctgcgt gccgcggctt gccatcggga tcgacgagat cagccgcgcg
     3541 cgcgtcttcg cggtgacgat ctcaccggcc ttcgccgagc gctccatcgt cgaatagccg
     3601 taatacggaa tcaccatgcg catgcgatac gcaccgaaca tgacgaggcc gcatgcgagg
     3661 tcgtagagct cgaccgcggc ttcgtcgtcg atcgtgccgc cgacgaagat cacgtcgcga
     3721 tcggcaggat cggtgtcgat acgccgatag tgctcgccgt ccgggaacgt cttgcgcgtg
     3781 atgacgccgc gctcccagcc accttccttc gcaatcgcgt cggcgaggta cgtgtaggcg
     3841 gccgtcgaaa atacgagtgg ctcatgcatg gcgcgctctc gcttccgcga tcagctgttg
     3901 cttgcgacga tgaacgtggc cgtcgagacc gacgggatat ggctgcggat tgagaaagcg
     3961 tttcgtgcgc ggcgagagca tcgcgagatc gtgcgcggcg cgcgctcgcg cggcttctag
     4021 atcgggcagc ggcaagacgc ggccgcgatc gatcacggtg gtcagcaagt cctcggcgtg
     4081 gtcgtgcacc ggcgcgaccg gcccccgcaa cggatcctcg atgtcgtgca gctcgcacgg
     4141 cgtctcgcag ccgtggtcgc tgtcgaagat gacgtcgccg acgtactcgt tgccgttgcg
     4201 gagccgtcgc acctgcagca tgccggggtt cgagatcttg atcggctgct ccgagagctt
     4261 gatcgcctcg cgccatgcac cgctgtcgtc gcgatacgcg ccgagcttgt acacgccgcc
     4321 gagcgccggc tgatcgaacg cggtgatgag cttcgtgccg acgccccaga tgtcgatgcg
     4381 cgcgccctga tcctggatgt tctggatcag atgctcgtcg agatcattgg acgcgacgat
     4441 cttcgcgttc gggaatccgg ccgcatcgag catcgcgcgt gcctcggcgg acagatgtgc
     4501 gaggtcgccc gagtcgaggc gaatgccggc gagctcgtga ccccgtgcgc gcagctcctt
     4561 gcccaccgtg atcgcgttgc gtacgccgtc gagcgtgtca taggtgtcga cgaggaacgt
     4621 gcagttgccg ggcagcgtct cggcgtatgt acgaaatgac gagagctcgt cgccgtggaa
     4681 catcacccag ctgtgcgcgt gcgtgccctt cacaggaatg ccgagcagct tgccggcgag
     4741 cgtgttcgac gttgcagcgc agccgccgat gtacgccgcg cgggagccgc cgagaccgcc
     4801 gtcgataccc tgcgcgcgcc gcaggccgaa ctcgagcacc gggcccttcg cgacctgcgc
     4861 gagccgcgcg gctttcgtcg cgatcagcgt ctgataattc acgagcgtga gcagcggcgt
     4921 ctcgatcaac tgcgcctgca cgattggacc gcgcacgcga atgatcggtt cgtgcgggaa
     4981 cacgagcgcg ccctcgggca ccgcatcgac gctgcacgag aatttcatct cgtgcaggta
     5041 gccgaggaac gcgggcggaa acagcggctt ctgctcgttg tccttcagcg tcgcgaggaa
     5101 cgcgatgtcg tcgggcatga agcgaaaccg cttcaagtac gcgaccaccg gggcaatgcc
     5161 tgcggcgatc gcgtagccgc cgccgaatgg cgggcgccga tacgtcagat gaaagacggc
     5221 ctcgcgctcg gcatggccgg acttccagta gccggcagcc atcgtgagct gatacagatc
     5281 ggtcagcagc gcgagcgaac cgccgtagac gtcatcgagg ccgtcggtca tcgttattac
     5341 tgtgtatcat ttacactaag tccttcgatg tcaaatggta gtgaccagcg cgcgcccgcc
     5401 gctaaacccg gtgcggaagc ggttatcagc gatcaagcgg tgatcgtctg gggcgaggtt
     5461 ctttgggacc gatttcccga cggcgataag ctcggtggcg cgccgtcgaa tgtcgcctgg
     5521 cacctcggtc ttgccggcgg ctgggcgcga ctggtgtcgc gggtcggcga cgacgcagca
     5581 ggccgcgagg cgatcgaacg cttgtcgaac gtatgtgatg ccgatttgat ccaaatcgat
     5641 tccgagcgtc cgaccggcga agtgcgtgtt gcgatcgaaa acggagagcc tcgctacacg
     5701 ctcgtccccg gctgcgcctg ggagcggatc gagtgcacgg ccgatgtcgc tgctgcgctc
     5761 ggcgaagcag gcgtgatggt ctacggcaca ctcgcacagc acaccgagac cggcatcgcc
     5821 ggctggcgtg cggcgattgc cgccgcacac ggaagttgtt tgaaggtctg cgacgtcaac
     5881 ttgcggcgcc tgtcgatcgc gccgccggtt gaacaacgcg cggtcaagga agcgatcgcc
     5941 gccgccgaca tcatcaaggt caacgatcag gagctcacgc gcatcgccga ctggttcggc
     6001 tggctcgatc cgctggccga gctgcgcgcc gggcgccgcg tcgtggccgt cacccacggg
     6061 aagaacggtg ccacgctgta cggcgacaag gagatcgtca cgattgcggg cgtgcagtcg
     6121 aagccaggtg gcgataacgt cggctgcggc gatgcctttg tcgccctctt cgtgcacggc
     6181 atgactctcg gctgggatct acgaacttcg ggcacggcag catgtcgatg ggcggctgcc
     6241 gtcgcgggtg tgcgtggcgc gacaccgatg ttcgacgaag agcaaatcgc cgagctgctc
     6301 ggagaggcgg cctcgtgagc gagccgaacg agtcaggcgg cagagcgtcc gtacggtcgt
     6361 cgagcaacat gggcacgacc accgagtcgc ggcaggcggc gcgcgtcgag aacaccgaag
     6421 acaaaccaag cgcaccggtg atcaagaagc gcgagtcgtt cttcgcgcgc cacctcggcc
     6481 gctacttcgg cggcagcacg gacaagaagc ctggcggcga gagtcccgat tcgtgggacc
     6541 aggtcgcggc gtggttcctc acgaacggcg cgctgccggc actcgcgctg atcaccgtcg
     6601 cggtgttcgg catccacgcg catatcttcc gaggcgaaac cgcgggcgac gatctgtcgt
     6661 tccactttgc ggagtcggcg cgcctgatgg actgcatccg ccacggcgac ttcgactttt
     6721 ggaacccgag cgcgaacgca ggctacgcgt ccgcctacta ctaccaggtg ctgccgcagc
     6781 tcgcgtcggc gctgccggcg gcgatcttcg gtcatcatct attctggttt cagctgagcg
     6841 tcgtgttgcc gctcgtcctc gcgccggcgg ctgcgtacaa aggcatgcgc ctgctcggcg
     6901 cgacgccatg gcagtcggtc gtcggtgcgt tctgcgtcgg gttcatgaac ggcgcctcgc
     6961 gctggggcgc gggcaacgcg ggcacgtttc aggtcggcct ctacacccag acgtgggcgc
     7021 tgtgcgcgtt cccgatggcg ctcggctacg cgtacctgtg gatgacgaag ggcaagcgcc
     7081 tcgcgtcgac aatcgcgtgg agcgcgttcg tcacgctgtg ccatccgttt gccggcatca
     7141 cgctcggatt cgccgcgctc gccgcgtggt tcgcgacgtt cgtgctgctg ccgttcgact
     7201 acttgttcgc agcgctcgcg ccgaagctga ccggccggct cacctggctc ggcaaacgct
     7261 gggcagagcc accgcggcga gcgaaggaaa tcctgcccga gctcgtgcgg ggcggcttgt
     7321 tcttcatcgg cctcgtgatc gccgtgatgc cagtctggct gccgctcgtg atcgatccgc
     7381 agggcttcgg cgggttcccg caccgcgtcg gcgacgaggt tggccctggc ttcaagacgc
     7441 tcgcgcactg gtacgcgaac ggcgagatcc tcgactggct cgcaccgagc gcgggcctcg
     7501 gcacgcggct cgggctgctc acgtggtcgc tgccactcgt cgtgatcctc gcgcgcgctc
     7561 catattaccg gtggctgtgg gcacccgcgg tgctctacgc gctactgctg ggcgtcggtc
     7621 cgaacatcgg caagatcggt gacgatctga tcccgccggt gcgcttcctc ggcggcatgc
     7681 agaccgtgct cgcgatgggc atcggcgccg gcgtcgtgct gatcggtcag cgtgcctggg
     7741 agatcgtcgg tcgctggccg tcgaaggcct atgcactacg gaccgtgatc gctgcgatcg
     7801 cagcagcagc gatcgtgctc gtcgtcgtgc cgggtggtcg cgcgctcggt gcacgcgtgc
     7861 gcgtgctcgg cgatcactcg aacagccacg ccgacgagct gttcctcgtc aacgcgatcc
     7921 tcgcgaggca gccgcagggt cgcaagcaaa ctggaccggg cgcggagaac cactggtgga
     7981 acctgctgtc gtacgtgtac gagcgcgtgc cgtcgacgct gcagatgggc ggcggcggtc
     8041 tgcaagcatc gccgaactac gacttcttgt ggacgcagcg cgacctggtc aagaacgcgt
     8101 ggatcttcga cgcgccgtat ctcgtctaca tgtcctcact cggcagcaag atgccgatcg
     8161 cggagatgat cgcgaagacg ccgaattacg aagtgcgccg gctgccttcg gcgggtctcg
     8221 tatcgccaat ccaggttgtc ggcgtgttgc cgccgggcta ttcgcacaag caggaaggtc
     8281 ataagaaggc gctcgagtgg atcaagagcg atcagccgat gaaggacgag atgctcgcgt
     8341 atgtcggctc cgagcacggc aacagcgatg cgcctgccgg acgaacgctg cgctcgtggc
     8401 ggcaagattc gccgggcagc gatcccgata tcgtcgccga ggtcgaggcc gagaagacga
     8461 cgacgttcgt cgtgcgcgag agctggcatc cgcgctggca cgcatacatc gacgaggatc
     8521 aagaagtgcc ggtgcgccgc gtgacgcccg acttcatcgc ggtcgacgtg ccgcccggca
     8581 agcacacgct tgcggtccgc ttcgagcggc cctggtgggc gcatgcgtcg tggctcgcgt
     8641 ggccgctgac cgtactgctc gcattcttcg cgatgcggtg gtggtcgcgc cgaaacgcaa
     8701 cgtcaccggt ggcctgagcc aaccgcggcg tccggacggc tcggcggcgg cggcgagccc
     8761 atctcgcgca tcagcggcgg ctcgtcgact tgtacgtcgg gggccggcgg aacgagcgag
     8821 cgttcctcgt cgggacgcgg catcggtacg acgaccttga ggctgcgcag ctgccacttc
     8881 gcggtcttcg acgggcgcga gaagttgaag atgatgcgaa caccgctcga cttctcgaac
     8941 tcggcgacag tgtcgaagaa cgcggtcagg ccgccgatcg atttcgcttc ggtcacggtc
     9001 aagaggcgct tgtagtcgcc gagtgggatc ttgttgtcct cgtagatgcg gatgaagttc
     9061 gcgcgcgtct cctgctgctt gaagatgtcg ctcgcgctgt catagacctc gccggccttg
     9121 ccgtcgcgaa tcatctcgag aatcgtctcg gcggcatcac gcacgtcgca tttcgcgggc
     9181 tttccatgcg cgtgatctgc gggttgatcg gaaacgtcgt gccctttctc gtcgagacac
     9241 gcggcaacac gcttctcgcg ttccgcctgc gtgatcttca ccgacgccgg gacctcgata
     9301 ccgataccga ggaacttcca cttaccgtcc ttgtccttgt ggaagctgat cgagcccttc
     9361 gtgatgccgt gctcgtagag cgcggtgagg ccgacgcgcg cgaccggacc ggtcggcccg
     9421 gtgccgacga cggtctcgtt gatcgcggtg atctcctgga acttgccgtt cgtcgcgtcg
     9481 aggtccttca tcaagtcgac gaagcgctcc ttgagcacga actcctgaaa acgcggcgac
     9541 gccttctcgt agacatcttc gatgccctgc ttgccggagc tgatctccat caggatcttc
     9601 tccgcctgtg ggcgaaagtc cggtgtggtg gcgctgcgca gctggatgaa cacgatcagc
     9661 gcgatccacg agaacacgat caagaaaccg acgatgacgc tgcgcacgag cagccggccg
     9721 cggcgtgtct gcgcgacgcg cggcaacgcg tgaatcgatt cgccggcctt cgtcagacct
     9781 tcgccgagct tgccgacgct ggcgccgacg agcggcacct tcttcgtgac gtcgccgact
     9841 ttcgcgacac cttcgccgat cgtgccgatg ctggcaccga gacgctcgac cgcgccgctc
     9901 gctgcatcct gcatcgcctt cgtgatgcgc ttgctgcgcc caccgacggg gccggtgtca
     9961 tgcgcagtcg cgaccgcgac gacgccaccg ctagcgacgg gcttggaatc ggccgcaggg
    10021 actgcttgcg gaattcccgg gtgcgagagc gcgacgtcga ccgagcccga cgcgctcgcc
    10081 ggcagttcgg gctgcgagag cttcacatcc accgagcccg acgcgcctgc cggaagctcg
    10141 ggctgcgaga ctttcacatc gacggaaccc gacgcgcttg cgggcatgtc ggtcttggca
    10201 ctgctcggat cgtcggagct cgcggccggc aattccggca gctccttgtc ccggtcatcc
    10261 ggtgcaccag ccactactac gacagtatgc ccgagaagaa ccgcgcgacc agattctcgg
    10321 ctacggcgag gcgatatacc gccgtcgacc gcacgtcgtc gatcggcttg atcgccgtgc
    10381 gcagcgccgc gcgtgcagct tcggccgcct ggtccggcgt gagcccacgt accgcgttct
    10441 cgaccgcagc gacgcgaatc ggccgatcgg caaccgcgcc gagtgcgacg cgggctgact
    10501 tcacgacgcc accgtcgagg tcgatcgccg ctgcgcccat caccttcgag atcgactgcg
    10561 cgcgccgcgt accgaccttg cgccacgtcg tgcgcgtcgt cttgctcggc gccggcaatg
    10621 tcgccccgac gatcagctcg tccggtgcga gcgcagtctt cttgtagccg gtgcagaaat
    10681 ctttgtaggc gatgcgccgc gtgccacgca ctgacgcgag ctccagctcg gcatcgagtg
    10741 cgagcaacac gggcaagcta tcgccgacgg gcgacgagtt gccgacgttg ccaccgagcg
    10801 tgccgcgcgc ctggatctgc agcgcgccga tctcgcgtgc cgcgagggcg agcggcaacc
    10861 agcgttcgag gattgcagtg tgcgtctcga cctcgtacca ggtcgcgccg gcgccgatcg
    10921 tgacgtgcga gtccgcggcg acgatgccgc cgagcccggg cagtcgccat agatcgagga
    10981 tgccggccgg tgccgggcga tgattcgcgt tgaccatcag gtcggtaccg ccggcgagca
    11041 gcatccagtc gggatgcttc gcgcgcaggt cgagcgcttc tttcagatca cgcgggcgtg
    11101 cgtagccggt cacgactcga cctcccgtgc tgccgcgatc accgcgtcga cgatcaattg
    11161 ataaccggtg caccggcaga tgttgcccgc gagtagctcg cgcactgccg cacgcgccgc
    11221 tgcttcgtcc ttgccgcgga tcaatccgcg atcgacgacg tgcgtcgcgc acaccgcgat
    11281 acccggcgtg cagatgccgc attgcgcgcc gccgcacgtg acgagctgtt tctgcaccgc
    11341 gtgcaagacg tcgccccgcg cgagcccttc gaccgtcgtc actctgcggc cgtcgacctg
    11401 ccctgccgcg acgagacacg agttcacgac ttcgccgtcg agcatcaccg agcacgcgcc
    11461 gcactccccc tcgccacaac cttccttcgt gccggtgagg tgcacgtctt cgcgcagcac
    11521 gtcgagcaag cgcttccacg gcgagcactc gaccgtgcgc ttctcgccat tgacctcgag
    11581 cgtgatcttc atcgacatct cagccgagcc tccagcgcgg gcggctcgcc gcgaccactt
    11641 caggcatgat cggaattgca tcgaactcgc agccgagcgc gttctcgacc gcattcgcga
    11701 cggcggcggc gggaccatcc atcggaatct cgccgacgcc cttcgcgccg ctcggcccga
    11761 acggatacgg cacctcgatg agaatcgtct cgagctccgg cgcatcagcg aacgtcggca
    11821 cgatgcagtt cgtcatgttc gcgttcatca ccttgccgtc cttgtagacg acgttctcga
    11881 gcagcgccca gccgagtgct tgcaacgtac cgccctcgat ctggcctttc acgatcaccg
    11941 gattgatcgc cttgccgaca tcgacagcct gcacgcagcg atcgacatgc acctcgtacg
    12001 tatcgagatc gaccgagacg tcgacgagac acgcggccca gccgtagcaa ggatacgccg
    12061 caccggtata cgtcgcgtcg tcgaaatgga tgccaggcgg tggctcgtac tgcatcgtca
    12121 ccgcgacttc cccgtcgtgt ggctctttca acttctcctt gagcgcgcgc gccgctttct
    12181 cgacgagccc gccgacgacc atgcacgtgc ggctcgcgac tgtcgggccg ctgtcgggca
    12241 cctgcgcggt cgacgggtcg acaacctcga cctgcgaggg atcgatgccg agcgcgccgg
    12301 ccgcgatctg cgtgaagatc gtgatcgcgc cctggccgat gtccgtcgag ctcgatcgca
    12361 cttcgtagcg accgtcgacg gtcttcgcga ccgttgcgcg gcccgcgagg cgttgctcgc
    12421 cgctgcctgt gaacccggcg ccgtggaaat aaaacccgac accccgaccg cgccgcacgg
    12481 gagcgccatt gcgcacgccg tccttccgcg gtgccggccc cgcgacgcgc tcgatcgcgc
    12541 cgatcacttc gtcagtgccg accgagaacc cgagcagctg accggtcgac gtcgtgtcgc
    12601 ccgaccgcag cgcgagccgc ttgcgcagcg ccatcggatc ttcgccgcgc gcgatcgcga
    12661 gcttctggat ctgacgctcg tacgcgaacg tcgtctgcgg tgccccaaag ccgcggaacg
    12721 caccgtgtgg cggatgattc gtcgcgacga cgcggccacg cacgcgcgtg accgggcagc
    12781 gatacggccc ggcggcatgc agcacaccgc gcgacagcac gaccggcgac agcgtcaggt
    12841 acgcgccgcc gtccatcacg acatcgacat ccatcgcgat gatctcgccc cgctcgtcga
    12901 ccgcgatgcg atgatgcgtg cgcgcgggat ggcgcttcgt cgtcgcggca atatcctcgt
    12961 cgcggtcgta gacgatcttc accgcgcgcc ccgccttctt cgcgagcacc gcgacgtgcg
    13021 ccgcgagcat cgacggatac tcttccttgc cgccaaatcc gccgccggtc accgattggc
    13081 tgattacgac atcgtcgggg ccgcagccga gcagcggctt catcgccttg tgcacgtaat
    13141 acgggcactg catcgacccg acgatgtagc aacgcccacc gtcccaccgg gccatcatgg
    13201 cctgcggctc gatgtacatc tgctcctgcg cgctcgtcca atacgtcccc tcgatcacct
    13261 gcgccgcgcg cgcgaacgca gcatcgaacg ccgcgtcgtc gtcgtgaccc ttgcggatca
    13321 agaactgctt gaacacgttg tcatcgccgt acagcttctg cgtcagcgcc aacgagtcgt
    13381 cgatcgtgaa cacgccgggc agcggcttca cgcgcggctt caccgccttc gccgccgcga
    13441 atgccagctc cttcgtcggt gccgcgacga gcgcgagcgc ctcgtccacg tgcatcacgc
    13501 gccccccaat cggcaccagc gcaacctgat cgttctcgat cagcgcgatg taattgctgc
    13561 ccggaatgtc cgcggccgtc acgaccgtca cctgcgacca gtcgaacgcc ggatccagat
    13621 cgatcgcctc cagcacaccg tgcgcgatct tcgaccgcac ggtcgcgccg aaccacgcgc
    13681 cctccgcctg caagtcatcg agatagcgcg cacgcccggt caccttcgca gcgccgtcca
    13741 cgcgcggaac agatgcgcca acaggcggct tcgcgtcaga aacgctgggg acccgatcca
    13801 tcccgagatt caatcacagc cgccgcggct tcactaccgc cgccgccccg agcttgccgt
    13861 aggatgtcgc gtcgatgtgg tcgcgcgcaa ttccggcgat tggttcaggt gacgtcagcg
    13921 tttcccgtgc catgaagcgc ggcctcgacg cctccgacgt ccgccgcgcc gtcgaccacg
    13981 tcatcgagtc cggcttggat ttcttcgacg tcgcggccga ggaatcggcc gaatcgctcg
    14041 tcgccgacgc gatccggtcg ctgcgcgtcc gcgatcgcgt catcgcggcc taccgcatac
    14101 cggcgatcgc gcagcgcctc ggcatcccga cccgtgacac gctgcccgag cgcctgccgc
    14161 cgcgttacat cgtcgaccgc gtcgaatcga tcctacgtgc gacacgcctc gacgccattc
    14221 cgctcgcgca gctcacgctc cgcgcgctct ggcgctcctc gtccgcgtgg cccgaggtca
    14281 ccggcacctg cgcgcgcctc gtgcgcgagg gcaaagtcct cgactgggcg gctttcgtcg
    14341 acgcgatcga ggatgacacc gtcgagctca cccacgagcc gtggctcgca gccatctcgg
    14401 tcccttactc gctctgcgag cgcgccgccg atccgatcct cgacgcggcc gcgctcgtct
    14461 ccgccgcacc gcaaaccacc gccgccgccg aacgcaagct caccatcctc gcccgccgac
    14521 cgctcgccgg tgccgcgctc gccggctcac tcggcccggg cgcgaagctg cgcatgcacg
    14581 acgatcgccg ctcgatcgac gacgcaacgc tggaacggat cgcggcgacc gtcgcgaagc
    14641 tcgcgcgctt cacgaagcaa acgccgcctg ccgcgttctc gtgcgatgcc gcgaaggcgc
    14701 agctcgaccg caacgtgcgc cccgagcact tgcacgtgca aacgcttgcc gagctcgccc
    14761 tccgctttgt cgtcacgcgc ggcgcgatcg cactacctcg cctgcatcgc aaagagcacg
    14821 tggccgacgc gctgatcgca gcccacagcc caccactccc gcgcgatctc atcgaatcga
    14881 tcgagaaact ggacatctaa acatttgttc agtaccgtcg tctgggtgaa ggctcgcccg
    14941 acggagaatc cgccgatccg gttccgcgac gtgaccgtcg cgtacgacga aggtgatggc
    15001 ccggcacccg cgaccatcac gctgctcgaa gatcagtcgc gctcgaccct ctcgcacaac
    15061 gactcgcccg acctcggctt caactggagc gcgaatccat atcgtggatg ccagtccgcg
    15121 tgccactact gctatgcccg tccgacgcac gagtatctcg atctcggcgc aggcaccgac
    15181 ttcgacacga agatcgtgat caagccgcgc gcgggcgaat tactccgcgc cgcgttcgac
    15241 aagccgtcgt ggaagggcga gctgatcatg ttcagcggcg tcacggactg ctaccagccg
    15301 atcgagcgcg accttcagct cacaaagcag tgtctcgagg tttgtctcga gtaccgcaat
    15361 ccagtcgcga tcatctcgaa gcacgcactc gttgaacgcg acgtcgatct aatcgccgag
    15421 ctcgcgcgcg aagcgagtgc tcatctcagt gtgagcctgg cgtggatcga cgcggagctc
    15481 gcgcgcacga tcgagccgtg ggcggcgtcg ccgcaacgcc ggctcaaagt catcgagacg
    15541 ttcgcgaagg ccggagtgcc ggtcggcgtg atgtgcgcgc cgatcattcc cggcctcaac
    15601 gacaaccaga tcgtgaagct gctcgaagcc gcgaaagacg cgggcgcgca gtgggccggc
    15661 tggacgctgc tgcgcttgcc cggcgtcgtg aaggaagtgt tcgaagatcg gctgcgcgtg
    15721 tcgctgccgc tcgtcgcgga caaggtcatg catcgcgtgc gcgagacgcg cggcggcgag
    15781 aagctctacg actcgcagtt ccacacgcgc ggccgcggcg tcggcgtcta cgccgaaacg
    15841 atcaaggcgc tgttcgagac aacagtgaag cgccttggcc tcaacgagcg cgattggggc
    15901 gaccgccctt cgcgctttca acgcccgaat cgcagcccac agctctcact gttctgattt
    15961 tgcgccgagg aaaccaatga cgaagctctg tcttctccag ctcgaggact gctcgaacgc
    16021 ctgcaatccg gcccatggct cacatccatg ccgtatccca cccgccgatc gagctagctc
    16081 gcgagaaccc agacgaggcc ggcgagcgcg acgggggctc tcaagcgtcg cgctttcgcc
    16141 gccccaatcg cccctgccgc tgtcactttt ctgatttacg cttcgggagc cagtgcaggc
    16201 gtagttcctt cttttccatg gcgcactcgc gcaaatacat gttgatcagc gtctggtacg
    16261 ggagccccac ctttgccgcg agctccttga aatactcgag cgtgtcgcga tcgatgcgaa
    16321 tcgtcactgc acgcttgagc tgccgcgcgt aagggttctt ccgcgccttc gaaaagtcgt
    16381 attccttccg catcagttca atccctcgag gtacgcgcgc cgctccctag cgctcgcctt
    16441 tcgcgccgag atgattcgga tcgcatcggc tcgatagcag tggcaaacga ccaagatgcc
    16501 gtacagcgag cggccgagaa gaatgaaccg gtcctcgaca tcggagtgat ccggatcgtc
    16561 gatgagcaaa gcgtattcgt cgatgaatac caaggcggcc tcttcgaacg aaacgccatg
    16621 tcgcgccatg ttgtgcgcgt tcttcgccgg gtcccattcg aatcgaatct cgtcgtccac
    16681 gccatacctc aattgtacat attttgtatg tacacgctag gcagtcgcgt ggcggcgatc
    16741 caagacgtgt gccgcttcgc cggcaatgaa tcccagcgac ctcgccgagc ggtgcgtccg
    16801 acccaaggac gaatgaccgg cttgcggccg gacgccgtac gccagcgctc gatgcgagcg
    16861 cgccgacgac ctacttcttt gcgggcgcct tggccggtgc cttcgcgggc actgccttcg
    16921 cgggcgtctt cgtcgcgccc cactcgcctg gttttggcgc cttcttcgga tctttctcga
    16981 acttgaacgc cttcgcctcg atcgatgccg tcgagcgcgg ttggttgctg cccaggcgcc
    17041 aggccgtcac ctcgccttcc gcgaacacga cgccaccctt ctgggtatcc atgcgcaggc
    17101 ggaagagcac cggctggcct tcaggcagct tgatctcggt cggcccggaa tcggcgttcg
    17161 tcgtctcggc gaaggcctcg aggcccttgg gcttcctcgg cgtcagctcg aacgagcgca
    17221 cgccgagctt ctctttcgtc gcggggtcga acagctcgac gagcaccgcc aacttaccgt
    17281 tggcgcgcga cacgtcgtcc ttcgccttcg cgaccgcgtt gccgatagcg gcgtcgatat
    17341 tcaccgagta ccagaagttc acgtcggtct gcgtgccgat gtcgggccac ttggcgtcga
    17401 cggcatcttg cttcgcgagg gcagcgccca tcagacggca cccgagcttg aacgcggacg
    17461 gcttcgagcc gagcacctga gacggcttgc tgacgccgac gacgccgagg aacttgccct
    17521 tcgtcgtcgg cagccagacg tgacactcga cttgcttgct gcgatcgagc ttgacctttc
    17581 tgtcgagctt cgcgatctcg accttcgcgc ccgtgcccac gtcgacttcg tcctcgagca
    17641 gccaggtggt ttcgcggtag tagcgcagct tgatctgcgc cttgccggtg agcgtgtcgc
    17701 cgtcgtcgag gcgcttcgtg agctggcgct gatcgatcga cgacatctgg agctcgaccg
    17761 gcgtctcttg atccgcggcc caggcaccgc gcgcttcaac caggccagcg agcacaacca
    17821 cgaccacaca gcagagcagt cggcgcatct cgttcctccc cgtgagccgc attagacgtc
    17881 gagtcgggtc cgcgattcaa tcaactcgcg agaatccaca cgaggccggc gagtgcgagc
    17941 gccatgatcg cgagcgcccc gtaaaagatg accgggttac cgcgcggcac ggccaccgac
    18001 ggcgccggcg ctggtttgag cggcggagcg tcggcgagca gcggccggaa cgccggcgca
    18061 tcgccttgca ggtgcttctc cgccggatcg cggaagcgca tcacgaccgg cccgagctcg
    18121 acgagacagc cgtcgcgcag cgccatgccg cctgcgccga ccggatcgcc gtcgaccttc
    18181 gtgccgttct tcgactcgag atcgacgatg cgcgtgccgt cccagccgcg ccggatctcg
    18241 gcgtgcgtgc gcgagagatc cttgtcgtcg atgatccagt tcgcctcgtc gccgcgaccg
    18301 atcacgagcg tcgattccgg cggctgcagc gcacgcttgg cgccggcacg cgcgccgcgc
    18361 tcgatctcga gcgtcggctg accggcgcta ccgaggaggt tgcgcatcag ctcgcgtgcg
    18421 aggctctcgg tgcgctgcac gggcgccgct tgtgcgccgg cgggcgcggg catgaggcgc
    18481 acgcgatagc cagcgacctc gagcgtgacg ccatcgccga tgtcgccgcc gtcgcgcggc
    18541 gcaccatcga ccttcacggc accgaccgca cgccagcgct tgccctcgac gcgcacgtgc
    18601 tcgcgctcag cggcgttggc gggcaggcga atgcgcgcgt tcgtcgcgct gccgatcacg
    18661 acgacggcat cgtcgacatc gaccgccggc agtgcagcct gccctgcttc gctgacctcg
    18721 acgcgcagca cgtgacgaga ctacttcgtc tgctgcttcg cttcttcggc tttgtactgg
    18781 tagtagcgct cgagcaccat ctttagatat tggcgcgtgt acgcgtacgg cggcacggtg
    18841 tagccgaact tctggagcga gccgaggccc gcgtgatagc cggcgatcgt gagcacgaga
    18901 tcgccgtcga gccggttcgc gagccagcgg aggaggcgcg tgccggccat gatgttcgtg
    18961 cgcggatcga acacatcgcc ttgcacgccc atgtcgacct cgacgtcggg gtggacctgc
    19021 atcaggcctt tgcagtccat cgccgagacc accttcgggt cgtagtcact ctcgaccttg
    19081 atcacggcgc ggatgagggg cactggaatc cggtacaggt ccgaggcttc ctggatgaat
    19141 gcgtcgtagc ggtggaagcg ctcgggcgat ttgtcagtcg cgccgacctt gtcgcagcgc
    19201 gggcagcgct cggcggaggc cttgctgccc ttcgggggct gactgtctag caccttcttc
    19261 cacttgccgc cgctcggctt gatgttcgtg aagtgcacga cgccgtcctc gtcggtccac
    19321 gaatagacgt cggcgtgggc tgcaacggcc aggccggcga cgagcatcat agcgaccagc
    19381 atccgcacga cgagaaatcg taccttgctc cctcggaggt cgcaaaagct gtatggtcgc
    19441 cccgcacatg gcagagccgc tcgaaaccga ctacctcgtc atcggatcgg ggattgcggg
    19501 tttcaatttc gcgctgctcg ccgccgaaca tgggcgcgtc gtcgtctgta cgaagaagcg
    19561 gccgaacgac acgaacacga actgggcaca gggcggcgtc gccgctgtgc tggcgccgga
    19621 cgacagcttc gagcagcacg tcagcgacac gctgaacgcg ggcgacgggc tgtgcgatcg
    19681 caagatcgtc gagctgtgcg tgaacgaggg gccagcgcag gtgaagcggt tgctcgacat
    19741 cggcgtgcgg ctcgcgcgcg actcggccgg ccagctcgac ctcggccgcg aaggcgcgca
    19801 cacgcatcat cgcgtcgtgc actacgaaga tgtgaccggc cgtgagatcc agcgcgcgct
    19861 gatcgcggcg gtgcaatcgc atccgaacat cacggtgctc gacagccaca tcgcgatcga
    19921 cttgttgtcg atggcgaagt acggcggtga tccggcgtgc ttcggcgcct acgtgctcga
    19981 cagcaggagc ggcgaggtca agacgatctg cgcgcgcgcg accgtgctcg cgaccggcgg
    20041 taccggcaag gtctacatct acacgtcgaa tcccgatgtc gcgaccggcg atggcgtcgc
    20101 gatggcgtat cgcatcggcg cggcgtgcgg cgatctcgag ttcgtccagt tccacccgac
    20161 ggtgctctac cacccgatgg cgggcagctt cttgatctcg gaggcgctgc gcggcgaagg
    20221 cggcgtgctc aagctcgcga acggcgagac gttcatggag aagtaccacc cgatgaagag
    20281 cctcgcgtct cgcgacgtcg tcgcgcgcgc gatcgacaac gagctcaaga agtcgggtgc
    20341 ggacgccgtg tacctcgaca tgacgcacct cgatccgggg ttcgtgcgcg gtcggttccc
    20401 gaacatctac gagcgctgtt tgtcgcttgg catcgacatc acgaaacagc cgattccggt
    20461 cgtgcccgcg gcgcactaca tgtgcggcgg cgtgaagacg gacgagaacg gcgcgacgac
    20521 gatccgcggc ctctacgcga tcggtgagtg cgcgttcacc ggtttgcacg gcgcgaaccg
    20581 gctcgcgtcg aactcgctgc tcgagggcat ggtgttctcg acgcgcgccg cctctgccgt
    20641 gcgagatgcg ccgcgcatcc gaccgtccca ggtcgcgccg tggtcgtccg gcaacgcgac
    20701 ggactccaac gacgcgatcg tcgtgtcgct caactgggaa gagatccgcc ggttcatgtg
    20761 gtcgtacgtc gggatcgtgc gatccgacaa gcgcatcatg cgggcgcacc
//
LOCUS       DASUFX010000376         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1310519, whole genome shotgun sequence.
ACCESSION   DASUFX010000376 DASUFX010000000
VERSION     DASUFX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310519"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..435)
                     /locus_tag="VFV99_15090"
     CDS             complement(109..435)
                     /locus_tag="VFV99_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060692.1"
                     /translation="MIVRDDVPTGVQLAQTVHAAGWSAQLPESQGAEPPTIAVALAAS
                     PAELADLASALADAGVPHVRVHEPDEPWRGALMAIGVVPTSRARVRRYLAHLRPARVR
                     GKDDRD"
     gene            587..>2498
                     /locus_tag="VFV99_15095"
     CDS             587..>2498
                     /locus_tag="VFV99_15095"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5060693.1"
                     /translation="MTTSSALPKQYDPSEVEPRWLSFWLENGFFHADEASPTVPYSIT
                     LPPPNVTGSLHIGHALGSTMQDILIRWKRMQGFNAMWMPGIDHASIAVHVLLEKDLKR
                     REQKTRFELGRDEFMKRAWAWKERSGNRIGEQEKLMGFSLDWARERFTMDDKSNRAVR
                     EAFVRLYDEGLIFRAKRMINWDPASETVVSDLEVDTAEENGSLWELKYPIVGGGGEVV
                     VATTRPETMLGDTAVAVHASDERYKALHGKMVELPLTGRQIPIVPFELVRDGKPWPDP
                     AFGSGAVKITPAHDPNDYDASQIANPNLAVLQVIDPKGRMQAPAPQKYIGMTVDEARK
                     AVVADLEAGGFLGEVKPYKVPRGRSQRSGAVIEPMLMDQWWVKAAPLAEKCVAAVEQG
                     KTKFVPELWTKTYMHWMTNIKDWCISRQLWWGHRIPAWYCGKGHITVARDEVAACKEC
                     GATDLRQDEDILDTWFSSALWPFSTLGWPEKTRELSTFYPNNVLVTGPDIIFFWVARM
                     MMMGIHFMGKVPFRTVYLTSIVTDENGDKMSKTKGNVIDPLDVVYGASLDVLLNRVDV
                     EKPPEPDKVKAAVKKHFGKGIPSMGADALRFALAALNTGSSRIRLSIERVEGYRNFIN
                     KLWNASRFALMNL"
BASE COUNT          457 a          778 c          862 g          401 t
ORIGIN      
        1 tcgtcgcggt gatcatgcga gagccagtgt cgcagcagca cgaggcgttg gttgatgcga
       61 gcgagcaacg tcttggtgtc gagagcgggg gcaggtttct cagcagcgtc agtcacggtc
      121 gtcctttcca cgcacgcgcg cggggcgcag gtgcgcgaga taacggcgta cgcgcgcacg
      181 cgacgtcggg accaccccga tcgccatcag cgcgccgcgc cagggttcat ccggttcgtg
      241 cacgcgcacg tggggcacac cggcgtcggc cagcgcgctc gcgaggtcag cgagctcggc
      301 cggcgaggcg gcgagcgcga ccgcgatcgt aggcggttcg gcaccctgcg attcgggcag
      361 ttgtgccgac cagccggcgg cgtggacggt ttgcgcgagc tggacgcccg tcgggacgtc
      421 gtcgcgaacg atcacgtagt gggcgatccc actgcggccg gcgttaatgc atgggcaaac
      481 cctaagaccg agaccgagac cgcgcaactg cgacttgcaa gcggagcgcg ttcgcccgtc
      541 gcaacggcct gaaatgatgc gttccgcgtg ctagcgtccg ctcgccatga ccacgtcgtc
      601 ggcgctcccc aagcagtacg atccgtccga ggtcgagcct cgctggctct ccttctggct
      661 cgagaacggt ttctttcacg cggacgaagc cagcccgacc gttccgtact cgatcacgct
      721 gccgccgccg aacgtcacgg gctcgctgca catcgggcac gcgctcggct cgacgatgca
      781 agacattctg atccgctgga agcggatgca gggtttcaac gcgatgtgga tgccgggcat
      841 cgaccacgca tcgatcgcgg tgcacgtgct gctcgagaag gatctcaagc gccgcgagca
      901 gaagacgcgc ttcgagctcg gccgcgacga attcatgaag cgcgcgtggg cgtggaaaga
      961 gcgcagcggc aatcgcatcg gcgagcagga gaagctcatg ggcttctcgc tcgactgggc
     1021 gcgcgagcgc ttcacgatgg acgacaagtc caaccgtgcc gtgcgcgaag cattcgtgcg
     1081 cctgtacgac gagggtttga tcttccgcgc caagcgcatg atcaactggg acccggcgag
     1141 cgagacggtg gtgagcgacc tcgaggtcga taccgccgag gagaacggct cgctgtggga
     1201 gctcaagtat ccgatcgtcg gtggtggtgg cgaggtggtg gtcgcgacga cgcgaccgga
     1261 gacgatgctc ggcgataccg cggtcgcggt gcacgcgagc gacgagcgct acaaggcgct
     1321 gcacggcaag atggtcgagc tgccgctcac cgggcgacag attccgatcg tgccgttcga
     1381 gctcgtgcgc gacggcaagc cgtggccgga tccggcgttc ggctcgggcg cggtgaagat
     1441 cacgccggcg catgatccaa acgactacga cgcgtcgcag atcgcgaacc cgaatctcgc
     1501 cgtgctgcaa gtgatcgatc cgaagggccg catgcaggcg cccgcgccgc agaagtacat
     1561 cggcatgacg gtcgatgaag cgcgcaaggc ggtggtcgcg gacctcgagg ccggcgggtt
     1621 cctcggcgag gtgaagccgt acaaggtgcc gcgcggccgc agccagcgca gtggtgcggt
     1681 gatcgagccg atgctgatgg atcagtggtg ggtgaaggcc gcgccactcg ccgagaagtg
     1741 cgtcgctgcc gtcgagcagg gcaagacgaa gttcgtcccc gagctgtgga cgaagacgta
     1801 catgcactgg atgaccaaca tcaaagactg gtgcatctcg cgccagctct ggtggggaca
     1861 tcgcattccc gcgtggtact gcggcaaggg ccacatcacc gtcgcgcgcg atgaggtcgc
     1921 ggcgtgcaag gagtgcggtg cgaccgacct ccgtcaggac gaggacatcc tcgacacgtg
     1981 gttttcctcg gcgctgtggc cgttctcgac gcttggctgg ccggagaaga cgcgcgagct
     2041 atcgacgttc tatccgaaca acgtgctcgt caccgggccc gacatcatct tcttctgggt
     2101 cgcccgcatg atgatgatgg gcatccactt catggggaaa gtgccgttcc gcaccgtgta
     2161 cctgacatcg atcgtcaccg acgagaacgg cgacaagatg tcgaagacga agggcaacgt
     2221 gatcgatcca ctcgacgtcg tgtacggcgc gagcctcgac gtgctgctca atcgcgtcga
     2281 cgtcgagaag ccacccgagc ccgacaaggt gaaggcggcg gtcaagaagc acttcggcaa
     2341 gggcatcccg tcgatgggcg ccgatgcatt gcgcttcgcg ctggccgcgc tcaataccgg
     2401 cagctcgcgc attcgactct cgatcgagcg cgtcgagggc taccgcaact tcatcaacaa
     2461 gctgtggaac gcgtcgcgct tcgcgctgat gaacctcg
//
LOCUS       DASUFX010000377         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2006172, whole genome shotgun sequence.
ACCESSION   DASUFX010000377 DASUFX010000000
VERSION     DASUFX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006172"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..2181)
                     /locus_tag="VFV99_15100"
     CDS             complement(13..2181)
                     /locus_tag="VFV99_15100"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5060694.1"
                     /translation="MHQLDQILSEVAGYHPSADVGLIRRAYQFAAQAHDGQTRKSGDP
                     YVTHPLAVAQVIAELKLDVASVCAGLLHDAVEDTSATVEQLAEQFGKEIAFLVDGVTK
                     LGKLPYSTREEQQAENFRKMLLAMARDIRVILVKLCDRLDNMRSLNHLPPEKQERIAA
                     ETMQIYAPLANRLGIQWIKVELEDLAFKYLYAGEYEQLASEIAKTRAERLEYIQHVEK
                     LIQKEMGDNGVPCEVYGRAKHLWSIYTKMKRTQRPFEEIHDAIGFRVITDSQMHCYQA
                     LGVAHSTWTPIPGRFKDYIALPKPNLYQSLHTAVIGPKGERIEIQIRTRDMNMVAEHG
                     IAAHWKYKEGKPLALEDDKKFAWLRQLMESQKELRDPTEFLESVKIDLFGDEVYVFTP
                     GGDVKALPKGSCPIDFAYAVHSSIGDHCSGARVNGMIVPLRYQLRNGDTIEIITSPNQ
                     KPNKDWLKLVKTARARTKIRYLLRQEQREKAVALGTDLIDKALRKHDTTIQRAEKQLK
                     HAAQELRCQTVDELIVQVGYGKVTVQQVLEKALPNLAKKAGEELAPKAESMLKTLLRK
                     VTRRTTSSGIKVAGEEDILVRFAKCCSPLPGDPIVGFITRGRGVTVHRRDCDKGLDLD
                     PDRRIDVEWDGHSKTQHEVALQVLCANKPGLLANISQSFSDQGVNISQAHCRTTEDGR
                     AVNTFHASVRDLDQLKQVIRSLSRIKGVFSVDRVSADVGT"
BASE COUNT          354 a          743 c          761 g          494 t
ORIGIN      
        1 ttggcggtta tctcaggtgc cgacgtctgc agagacgcgg tcgacgctga acacgccctt
       61 gatgcgcgac agcgagcgga tcacctgctt gagctgatcg agatcgcgca cgctcgcgtg
      121 gaacgtattc acggcgcgac cgtcctcggt cgtgcggcaa tgcgcctgcg agatgttgac
      181 gccctggtcg ctgaacgact gggagatgtt cgcgagcagg cccggcttgt tcgcgcacag
      241 aacctggagc gcgacctcgt gctgggtctt gctgtggccg tcccactcga cgtcgatgcg
      301 gcggtcggga tcgagatcga gacccttgtc gcaatcgcgg cggtgcacgg tgacgccgcg
      361 gccgcgcgtg atgaagccga cgatcggatc gccaggtagc gggctgcagc acttcgcgaa
      421 ccgcacgagg atgtcctctt cgccggcgac cttgatgccg ctcgacgtcg tgcgccgggt
      481 gaccttgcgc agcagcgtct tcagcatgct ctcggccttg ggcgcgagct cctcccctgc
      541 cttcttcgca aggttcggca gtgccttctc gagcacctgc tgcacggtga ccttcccgta
      601 gccgacctgc acgatcaact cgtcgacggt ctggcagcgc agctcttggg ccgcgtgctt
      661 gagctgcttc tcggcgcgct ggatcgtcgt gtcgtgcttg cgcagcgcct tgtcgatcag
      721 gtcggtgccg agcgcgactg ccttctcgcg ctgctcctgc cggaggaggt aacggatctt
      781 cgtgcgcgcg cgcgccgtct tcacgagctt gagccagtcc ttgttcggct tctggttcgg
      841 gctcgtgatg atctcgatcg tgtcgccgtt tcggagctgg tagcgcagcg gcacgatcat
      901 gccgttgacg cgcgcgcccg agcagtgatc gccgatcgag ctgtgcacgg cgtatgcgaa
      961 gtcgatcggg cagctgcctt tgggtagcgc tttgacgtcg ccacccggtg tgaacacgta
     1021 gacctcgtcg ccgaagaggt cgatcttcac cgactcgagg aactcggtcg gatcgcgcag
     1081 ctccttttgc gactccatca gctgccgcag ccaggcgaac ttcttgtcgt cctcgagcgc
     1141 gagcggcttg ccctctttgt acttccagtg cgccgcgatg ccgtgctcgg cgaccatgtt
     1201 catgtcgcgc gtgcggatct ggatctcgat gcgctcgccc ttcggcccga tcaccgcggt
     1261 gtgcagcgac tggtagaggt tcggcttcgg cagcgcgatg tagtccttga agcggccggg
     1321 aatcggcgtc catgtcgagt gcgcgacgcc gagcgcctga tagcagtgca tctggctatc
     1381 ggtgatcaca cggaagccga tcgcgtcgtg gatctcttcg aatggacgct gtgtccgttt
     1441 catcttcgtg tagatcgacc acaggtgctt cgcgcggccg tagacctcgc acggcacacc
     1501 gttgtcgccc atctccttct gaatgagctt ctcgacgtgc tggatgtact cgagccgctc
     1561 ggcgcgcgtc ttcgcgatct ccgacgcgag ctgctcgtac tcgcccgcgt agagatactt
     1621 gaacgcgagg tcttcgagct cgaccttgat ccactggata ccgaggcggt tcgcgagtgg
     1681 cgcgtagatc tgcatcgtct cggcggcgat tcgctcctgc ttctcgggcg gcaggtgatt
     1741 gagcgagcgc atgttgtcga ggcgatcgca gagcttcacc aagatgacgc ggatgtcgcg
     1801 cgccatcgcg agcagcatct tgcggaagtt ctccgcctgc tgctcttcgc gcgtcgagta
     1861 cggcagcttg ccgagcttcg tgacgccatc gacgagaaac gcgatctctt tgccgaactg
     1921 ctcggcgagc tgttcgacgg tggcactcgt atcctcgacg gcgtcatgga gcaggccggc
     1981 acagacgctc gcgacgtcga gcttgagctc ggcgatcacc tgcgcgaccg cgagcggatg
     2041 cgtgacgtat ggatcgccgc tcttgcgggt ctggccgtcg tgcgcttgcg ccgcgaattg
     2101 ataggcccgg cggatcagcc ctacgtctgc gctcgggtgg tacccggcca cctcgctcaa
     2161 gatctggtca agctgatgca tcacgactgc gtgagaccct atctagggta ccacgcagcg
     2221 cggcatccgg cttactcggc ggcgacgttg tcctggaaac gccaggtgaa ttcgaatgct
     2281 tcgcccggtc gcttcacgat cagcttgagc gaccggacgt tctgcgaaaa cagatcacgc
     2341 tggtagaaca cg
//
LOCUS       DASUFX010000378        13038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1728712, whole genome shotgun sequence.
ACCESSION   DASUFX010000378 DASUFX010000000
VERSION     DASUFX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728712"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VFV99_15105"
     CDS             <1..1357
                     /locus_tag="VFV99_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX5060695.1"
                     /translation="YVLPGCQDTGTAIVMGKKGQHVVTNGADEEALSRGVFQTYTETN
                     LRYSQVAPLDMFEEKNTGTNLPAQIELYAVDGDEYHFLFMAKGGGSANKSLLFQETKA
                     LLNPKSLATFLDQKLRSLGTAACPPYHLAVVIGGTSAEFTLKVAKYASARYLDHLPTK
                     GTTAGHAYRDLEWEQKVVEIARATGIGAQFGGKYFCHDARVIRLPRHGASCPVAIAVS
                     CSADRQALGKITKDGVFLEQLETDPAQYLPDITHEDLEADAPAVKIDLTMPMNEIRAT
                     LSRYPIRTRLSLTGPMIVARDIAHAKLKERIDRGEGLPQYMKDHCVYYAGPAKTPAGY
                     ASGSFGPTTAGRMDSYVDLFQQHGGSMVMLAKGNRSQQVTDACKQYGGFYLGSIGGPA
                     ARLAKDCIKSVSVHEYPELGMEAVWKIDVVDFPAFIVVDDKGNDFFAGISGGGKKLPI
                     K"
     gene            1387..2208
                     /locus_tag="VFV99_15110"
     CDS             1387..2208
                     /locus_tag="VFV99_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060696.1"
                     /translation="MIRNSLLVVAALTAVADAQPGAQLQVSGDASAQVNLDQLVAQAE
                     AAAPSIETVVRGTVRRARRAISIGPTVGLWSAAIIDPGDIDAALTFGIGFETFKVPVL
                     PDIDTLRALIQERVKAQLKDRIKAVFQGRPPDPLELNAIAAQVYLDVRDEILGVKNVR
                     GKTMERPQFTVGFEANRLFGAERWLGRTRVGIGVWKVTLALSTTVGRVCRGAGCDDNV
                     KVFIGPEVVAHFMTSKSPRASVIDAFLRADFQANGRGIETYDQLTIGVRYLLDLI"
     gene            complement(2209..2916)
                     /locus_tag="VFV99_15115"
     CDS             complement(2209..2916)
                     /locus_tag="VFV99_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060697.1"
                     /translation="MSWRGVSWRAIAAAACALAATGCDFVFRIDHIPEQRIDADVRPE
                     DGAGVDADQLCASPVINTALDGTVPCAPWGMKYEDFGATVTSGSSGLVIKPQNQFNSV
                     AGCYSSNSQPFGIGGVKAHVTDLVRGAAVYTTLQVHGPDLEIKASGTGVVVFQTTNNV
                     QLGMAVISELPMWWRIRPVGTVDVIGEISPDGESWTMLGTASPGPPAPVGVELNAGVD
                     NGFTGGQSVFDRLVVCP"
     gene            3069..4289
                     /locus_tag="VFV99_15120"
     CDS             3069..4289
                     /locus_tag="VFV99_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5060698.1"
                     /translation="MFVGIDFGTTNSALAIADARGDVQLVPLAGASYWRTVLYFEPGG
                     GLTAGAPAIARYLETEGEGRLVQSIKSHLASAAFSRTSIFGRRWQLDDMIAAFVRQLR
                     AAAPFPLGTRCVIGRPVRYWGAEDEADDARAVGRMTEALAKAGFEEVVFEYEPVGAAA
                     RYAARLDHDELIVVADYGGGTTDFSVIRVGPGRARVLATGGIGVSGDAFDARVIDAIV
                     APALGRGSRYRDEMGGEAPVPAWLYGHLRRWHLLSFLKEESTQRLLERVVRGALEPIR
                     VARLVSVVEDDLGLALHRAVEGAKVRLSKRDADRVELPVITLDLPLARPAFDDWIADD
                     LASIDRVLDDVLARAGVDAREVDRVFATGGSSLVPAVRGRLAARFGDDRVVGGEELTS
                     VAWGLAARAREVFG"
     gene            4327..4665
                     /locus_tag="VFV99_15125"
     CDS             4327..4665
                     /locus_tag="VFV99_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060699.1"
                     /translation="MLRFLASLSLLALVACQPAPVGVRNLSVAPDSGPQCASLCQQVG
                     MSLDSLVIMADNVGCVCRAAGPAAPPGAEPGPAPAATTGAASAGGMAAILIQRQAQQA
                     AAQRSAAQRK"
     gene            4730..5203
                     /locus_tag="VFV99_15130"
     CDS             4730..5203
                     /locus_tag="VFV99_15130"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5060700.1"
                     /translation="MSSDAVHSPTPPSANDNLRCVETYIACTPDVCWRALTDASLLAA
                     WVPGLRRARVIDRDENGRAREVLFEFSASLTYSLVYSYDVPTRELRWEPRMGKRDGVR
                     GYARLDACDTGTRMTYALEQGPGRSPSDLALGDPEALLVAFARWVESRLAFQRAT"
     gene            complement(5206..6066)
                     /locus_tag="VFV99_15135"
     CDS             complement(5206..6066)
                     /locus_tag="VFV99_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5060701.1"
                     /translation="MRLVAVVLALTVTVVACGGKSQPRSDDETDGPFVNRAGFQPTAF
                     TAKVTGQGRPVIFIPGLACPGEVWDDTVARLGNEIQAHVITLSGFAGTKPIKPPLVAK
                     TRKELVRYIRSNHLKNPIIVGHSLGGFIAYWLAATAPDAVGGVVVVDAGPRYFADDEE
                     ARLLRNTWAQAGDDELPVALRNVFSGMTRDRRHIEPFIDQIAKSDRQTIGDAIYELVK
                     TDITPETASITAPVLLVLADGGLQARYRKQAADVDHLEVVVMPKTGHFVMLDDPERFA
                     RTLSAFIAKQ"
     gene            6109..7404
                     /locus_tag="VFV99_15140"
     CDS             6109..7404
                     /locus_tag="VFV99_15140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5060702.1"
                     /translation="MRAFWMIGLALVACGDSDNDGDPKQFIDQGGVLAIDGCNYMLTT
                     RIGAEQPVPSGKTIGMEPTPRQVHLGIMGDPKTSIVAQWRTADETTTVTTLRYGTGEG
                     LSEAQLTNIVKGVHFRYNSTGTDLYRIHQAHVCGLEPGTTYSYQVGADKHWSPVYTFH
                     TAPDVDAHPDAEVVLAFVGDSRGGYDVWTQLVDQIKQRTPDLVLFSGDAITIGLTQYE
                     WEEFLTDGEPLFATTPVVLTNGNHEANAINYFSQFAMPGDQENFGFDYGFAHVFVAND
                     SPDNPSDLTDVIPVAMDADFTDHTSARWKMLMSHRPMYSSGTRHGSAMDLQAAWGPVV
                     DKHKVDLVLNGHEHMFEISKPLFNNAVQASNADGTVYVVAGGAGAELYGFGTPGFWTD
                     YIESAHTAATIRVRRDQLTLDSFRPDGTAVPNGFTKTKP"
     gene            7401..8510
                     /locus_tag="VFV99_15145"
     CDS             7401..8510
                     /locus_tag="VFV99_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060703.1"
                     /translation="MKLVALAALLLLARVAAADDADAERTFRDASLRGDITALEQLGA
                     QRPLTIWSDDAWAEAARLATRANDFTRARADLEQVIAIGTDDRLVQRAKNELARITSF
                     SGSAGEWTAVATEHERLAPQLTAGGDPKPALRELEQLVRAHPGYPRAAMLMVAIAQSW
                     QREGEGERAIGWLHEAARVATERTDKLRIGAELVRALIRVGAFADAQRELAALDAGPA
                     LVAALRAELSRAELRRTVRWIMWGVLVALAVIAAIALRRAAGSWRAGLRRLARPPTEA
                     LFLVPIAAVVVGFSFTGNPLVARTVLAILAAGVATSWVSGAVLEGQPRVRFRRALLHA
                     SLAIVAVVAAAYLAVDRGHVIDFIIETLRSGHERG"
     gene            complement(8511..8981)
                     /locus_tag="VFV99_15150"
     CDS             complement(8511..8981)
                     /locus_tag="VFV99_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5060704.1"
                     /translation="MRIYLVRHGDAVPEEDAGSDRDRWLSPRGREAARILGRLLREQH
                     IAPDLIVCSPLPRAVQTAELLAQSIDYIGAISSWRCLEPAAMPRIAAGQIAQAGMSVL
                     VVGHEPSISSLGAYIMGRPSFPPFRTAQCFALEDGKPTFTARSDLDQIQPYFIE"
     gene            complement(8990..10513)
                     /locus_tag="VFV99_15155"
     CDS             complement(8990..10513)
                     /locus_tag="VFV99_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060705.1"
                     /translation="MLTPLSRIALVILLFASCGKGGNKNGKKDAASAPVAAPISLPVL
                     GVDKITRFSFIYDQGAAAYKKAADAAAKKDWAEARKQAEAALAKDPNHLDAHRVLAIA
                     LVQTGEPAAAVDHLVAAIAADYYKYGAELATIPELKDFLATQHGQAVTQLAAQIHDEY
                     AREIKAGLWLVGRRNSFKWPDKPGVQSGLSKGELYAFDRDSKRYLRLTHTGDQVAGFV
                     RAKQGNEVAILGYDKIDRSKEDAPPLLARGWVQAFDTAEWKNTSPKINLPSARAAALY
                     YGDGDQLLVATAPATGRWTIGDWTVSSIDRTTGKLTKVTAPLGSPRIELTLDEGRIVP
                     PKSIEGVGAAWIGDPPRAPTLELAKGVPPVQVPESGQAAMETVAVAPGGGYVAFATAV
                     DPCAKDTAPSLYVANGKTAALKHLLTAKSRFATTWLDPNTLAYEDGDGAIRLWDATTG
                     REALRLENKPGLALDVLSLASAPLCKQAPPTAETGSGGDEPPLPPEEGSDAPVTKPQ"
     gene            10538..11077
                     /locus_tag="VFV99_15160"
     CDS             10538..11077
                     /locus_tag="VFV99_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5060706.1"
                     /translation="MMIAFVLAAVVGCSKDKKVAKPTPTTAKPVAAKPADDKLLETLP
                     ADQQVSPNLSLSGDIVQMCGIKATANATAAPTFDYDKEELHPEDRAVLDQLATCLMTG
                     ALKGRSVALTGRADPRGTEEYNLGLGSRRAASVSTYLVRLGVGEPQLGVTTRGSLDAS
                     GTDEAGWAKDRRVDITLAN"
     gene            complement(11074..11568)
                     /locus_tag="VFV99_15165"
     CDS             complement(11074..11568)
                     /locus_tag="VFV99_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060707.1"
                     /translation="MLARVVVVLVFVACAAPQEPRIRRAEVVITSRAEVPAEYQKLLP
                     KHGVFVTGGGEKSPVYRIVVDTDAKTIYTGKAPAATPLTGALAEERTRELTPPNEQHL
                     MQLAQEAKAEPPPAQPPERVQGYDEIFAVADGDSVFFVQGRGPITRPKTIKAIEAFRA
                     AAAL"
     gene            11605..>13038
                     /locus_tag="VFV99_15170"
     CDS             11605..>13038
                     /locus_tag="VFV99_15170"
                     /inference="COORDINATES: protein motif:HMM:NF040100.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060708.1"
                     /translation="MALEHVAQGQTRFFTPGVAPDPRGILLGRFCLMTFPTLEGAVSW
                     LRLYSAEASLDELMANLTLTKAKTALGSREIVIQIPAVSSYAADRSARLARLVGGAIY
                     TGTSKHFVKYRDDRSPYGYDAVDIGAMPASTDFMVHGDEFAQGYVREGELPFAKLLFR
                     LSIRRIPGADKLATEDRGELYLAVAHGLSEGIIRYLWRNRVEAQAGLFTPRSSSAFDE
                     QRRGYMLIRARALPERILALFLGTPGIDVFKPAGANAAVAVGYQHPIDLASCSSVFPP
                     DTFHVFWPNDRVDVLPGPLTLSDIADLTRVDLELDKPRDPAQHGGGAAEPIGVELRLA
                     AAMGPPKRVVATLIEPKHAPRLKRIVFALPNVSLRGQRVAVTDRGILIIGSENLDVIP
                     LGQLLYELTPGLLVPLGMDIVPRVTPEVLARALGHQGGVVTVFTSDNRPFQVPDSAFT
                     SLERRSLAKMDVEAAETVELGAEPSAEA"
BASE COUNT         2080 a         4351 c         4435 g         2172 t
ORIGIN      
        1 ctacgtgctg cctggctgcc aggacacggg aacggccatc gtcatgggca agaagggcca
       61 gcatgtcgtg acgaatggcg ccgacgagga ggcgctgtcg cggggcgtgt tccagacgta
      121 cacggagacg aacctccgct actcgcaggt cgcgccgctc gacatgttcg aggagaagaa
      181 caccggcacg aacttgccgg cgcagatcga gctctacgcc gtcgacggcg acgagtacca
      241 cttcctgttc atggcgaagg gcggcggctc ggcgaacaag agcctgttgt tccaggagac
      301 gaaagcgctg ctcaatccga agagcctcgc aacgttcctc gatcagaagc tgcgctcgct
      361 cggcaccgcc gcgtgcccgc cgtatcacct cgcggtcgtc atcggcggca cgagcgcgga
      421 attcacgctg aaggtcgcga agtacgcgtc ggcgcgctac ctcgatcacc tgccgacgaa
      481 gggcaccacc gcggggcacg cgtatcgcga tctcgagtgg gaacagaagg tcgtcgagat
      541 cgcgcgcgcg accggcatcg gtgcgcagtt cggcggcaag tacttctgcc acgacgcgcg
      601 cgtcattcgc ctcccgcgcc acggcgcgag ctgcccggtc gcgatcgcgg tgagctgctc
      661 ggccgatcgt caggcgctcg gcaagatcac gaaagatggc gtattcctcg agcagctcga
      721 gaccgacccg gcgcagtacc tgcccgacat cacccacgag gacctcgagg ccgacgcgcc
      781 agcggtgaag atcgatctca ccatgccgat gaacgagatc cgcgcgacgc tgtcgcggta
      841 cccgatccgc acgcggctgt cactcaccgg accgatgatc gtcgcgcgcg acatcgcgca
      901 cgcgaagctc aaggagcgca tcgatcgcgg cgagggcctg ccgcagtaca tgaaggacca
      961 ctgcgtgtac tacgcgggtc cggcgaagac gccggcgggc tacgcgtcgg gctcgttcgg
     1021 gccgacgacc gcgggccgca tggacagcta cgtcgatctg ttccagcagc acggcggcag
     1081 catggtgatg ctcgcgaagg gcaaccgcag ccagcaggtc accgacgcgt gcaaacagta
     1141 cggcggcttc tacctcggct cgatcggcgg cccggcagcg cggctggcga aggactgcat
     1201 caagagcgtg tcggtgcacg agtatcccga gctcggcatg gaggcggtgt ggaagatcga
     1261 tgtcgtcgac ttccccgcat tcatcgtcgt cgacgacaag ggcaatgact tctttgccgg
     1321 aatttccggt ggcggaaaga agctacctat caagtaagct agatcagccg tcggtaagat
     1381 gagctgatga ttcgcaatag cttgctcgtg gtggcggccc tgactgccgt cgcggacgcc
     1441 cagccgggtg cgcagctgca ggtctccgga gatgccagcg cgcaggtcaa cctcgaccag
     1501 ctcgtcgcgc aggccgaggc ggcagcgccg tcgatcgaga ccgtcgtgcg tggcaccgtg
     1561 cggcgcgcgc ggcgcgcgat ctcgatcggt ccgacggtcg gcttgtggag cgcagcgatc
     1621 atcgatccgg gcgacatcga cgcggcgctc acgttcggca tcggcttcga gacattcaag
     1681 gtgccggtgc tgcccgacat cgacacgctg cgcgcactga tccaggagcg cgtgaaggcg
     1741 cagctcaagg accggatcaa ggccgtgttc caaggccggc cgcccgatcc actcgagctc
     1801 aacgcgatcg cagcgcaggt ctacctcgac gtgcgcgacg agatcctcgg cgtgaagaac
     1861 gtgcgcggca agacgatgga gcggccgcag ttcacggttg gcttcgaggc gaatcggttg
     1921 ttcggcgccg agcgctggct cggccgcacg cgtgtcggca tcggcgtgtg gaaggtgacg
     1981 ctcgcgctgt cgacgacggt cggtcgcgtg tgtcgcggcg cgggctgcga tgacaacgtg
     2041 aaagtgttca tcggtcccga ggtcgtcgcg cacttcatga cgtcgaagag cccgcgcgcg
     2101 tcggtcatcg acgcgttctt gcgcgccgac ttccaggcga acggccgcgg catcgagacc
     2161 tacgatcagc tcacgatcgg cgtccgctac ctgctcgatc tgatctgatc acggacagac
     2221 aacgagtcgg tcgaacacgc tttggccgcc cgtgaatccg ttgtcgacgc cggcgttcag
     2281 ctcgacgcca acgggcgccg gaggtccggg agaggcggtg ccgagcatcg tccagctctc
     2341 accgtcgggc gagatttccc cgatgacgtc cacggtgccc acgggccgga ttcgccacca
     2401 catcggcaat tccgaaatca cggccatccc gagttggacg ttgttcgtcg tctggaacac
     2461 gacgacgccg gtcccgcttg ccttgatctc gagatcggga ccgtgcacct gcaacgtggt
     2521 gtatacggcg gcgccgcgca cgagatctgt gacgtgcgct ttgacgcctc cgatgccgaa
     2581 cggctgactg ttgctcgagt agcagccggc gacgctgttg aattggtttt gcggtttgat
     2641 cacgaggccg ctcgatccac tcgtgaccgt cgcgccgaag tcttcgtact tcatgcccca
     2701 cggcgcgcac gggacggtgc cgtcgagcgc ggtgttgatg accggcgatg cgcatagctg
     2761 gtcggcgtcg acacctgcgc catcctctgg acggacgtcg gcatcgatgc gctgctccgg
     2821 gatgtgatcg atccggaaca cgaagtcaca gcccgtggcg gcaagcgcgc atgcggcggc
     2881 agcaatcgct cgccacgaga ctccgcgcca agacattcac gcgatcgtac aggaatgccc
     2941 agctccggcg aggcgccggt catgagcggc ggccaatcga cggcgccacc tcatcccgag
     3001 cagctcccac aggataggca gactggtgca gctagtaaat caaaatcgaa tcgccgacgg
     3061 tggagcccat gtttgtcggc attgatttcg gcaccacgaa ctcggcgctc gccatcgccg
     3121 acgcgcgcgg tgatgtccag ctggtaccgc tggccggcgc cagctactgg cgcaccgtcc
     3181 tctacttcga gcccggcggc gggctgaccg caggagcgcc ggcgatcgcg cgctacctcg
     3241 aaaccgaggg cgaaggccgg ctcgtgcagt cgatcaagag ccaccttgcg agcgcggcat
     3301 tctcgcgcac gtcgatcttc ggccggcgct ggcaactcga cgacatgatc gcggccttcg
     3361 tgcgccagct gcgcgccgcg gcgccgtttc cgctcggcac gcgctgtgtg atcggccggc
     3421 cggttcgcta ctggggtgcc gaggacgagg ccgacgacgc gcgcgcggtc gggcggatga
     3481 ccgaggcgct cgcgaaggcc ggcttcgagg aggtcgtgtt cgagtacgag ccagtcgggg
     3541 ctgccgcgcg gtacgccgcc cgcctcgatc acgacgagtt gatcgtcgtt gccgactacg
     3601 gtggcggcac gaccgacttc tcggtgatcc gcgtcgggcc cgggcgcgct cgggtgctgg
     3661 cgaccggcgg tatcggcgtg tcgggcgacg cgttcgatgc gcgggtgatc gacgcgatcg
     3721 tcgctcctgc gctcgggcgg ggatcccgct atcgcgacga gatgggtggc gaggcgccgg
     3781 tgccggcgtg gctctacgga cacctgcggc ggtggcactt gctgtcgttt ctcaaggagg
     3841 aatcgaccca gcgcctgctc gagcgcgtcg tgcgtggagc gctggagccg atccgcgtcg
     3901 cacggctggt cagcgtcgtc gaggacgacc tcgggctcgc gctccaccgg gcggtcgagg
     3961 gcgcgaaggt gcggctgtcc aagcgcgacg ccgatcgggt cgagctaccg gtaatcacgc
     4021 tcgatctacc gctcgcgcgg ccggcattcg acgactggat cgccgatgac ctcgcgtcga
     4081 tcgatcgcgt gctcgatgac gtgctcgcgc gggctggcgt cgacgcccgc gaggtcgatc
     4141 gcgtgttcgc gaccggcggc agctcgctcg tcccggcagt tcgcggccgt ctcgccgctc
     4201 ggttcggcga cgaccgcgtg gtcggcggcg aggagctgac gagcgtcgcg tggggactgg
     4261 cggcgcgtgc gcgcgaggtg ttcggctagc gaatctactg tcagacctgg tgcatagcat
     4321 cgcgacatgc tgcgcttcct cgcttcgctc tcgctactcg cgctcgtcgc gtgtcagccc
     4381 gcgccagtcg gcgtccgcaa tctctcggtc gcgcccgatt cggggccgca gtgcgcgagc
     4441 ctgtgccagc aggtcggcat gtcgctcgac tcgctcgtga tcatggccga caacgtcggc
     4501 tgcgtgtgcc gcgcggctgg gccggccgca ccaccgggag ccgagcccgg accggcaccg
     4561 gcggcgacga ccggcgcagc gtcggcggga ggcatggcgg cgatcctgat ccagcgccag
     4621 gcgcagcagg cagccgcgca gcgatcggcc gcgcagcgca aatagtgctg gatctcctgc
     4681 gacgaaatcg tcacacactc atagctgagc ccaccgtaga gtaagcaaat tgtcttctga
     4741 cgcagtccat tcaccgacgc cgccatccgc gaacgacaac ttgcgctgcg tcgagacgta
     4801 catcgcgtgc acgcccgatg tttgctggcg cgcattgacc gatgcatcgc tgctcgcggc
     4861 gtgggtgcct gggcttcgcc gcgctcgcgt gatcgatcgc gacgagaatg gtcgcgcgcg
     4921 cgaggtgctg ttcgaattct ccgcgtcgct cacgtactcg ctcgtctatt cctatgacgt
     4981 accgacgcgc gagctgcgct gggaaccacg catgggcaag cgcgacggcg tgcgcggcta
     5041 cgcgcgactc gacgcctgcg ataccggcac gcgcatgacg tatgcgctcg agcaaggtcc
     5101 gggtcgcagc ccctcggatc tcgcgctcgg tgatccggaa gcgctgctcg tcgcgttcgc
     5161 gcggtgggtc gagtcgcgtc tggcgttcca gcgcgcgacc taacgctact gcttcgcgat
     5221 gaatgcggac agcgtgcgcg cgaagcgttc gggatcgtcg agcatcacga agtgcccggt
     5281 cttcggcatg acgacaacct cgagatgatc gacgtcggcc gcctgtttgc gatagcgagc
     5341 ttgcaagccg ccgtcggcga gcacgagcag caccggcgca gtgatcgacg cggtctcggg
     5401 cgtgatgtcg gtcttcacga gctcgtagat cgcatcgccg atcgtctggc gatccgactt
     5461 cgcgatctga tcgatgaacg gctcgatgtg cctgcggtcg cgtgtcatcc ccgagaacac
     5521 gttgcgtagc gcgaccggta gctcgtcatc gccggcctgc gcccacgtgt tgcggagcag
     5581 gcgcgcctct tcgtcatcgg caaagtagcg tgggccggca tcgacgacga ccacgccacc
     5641 gacggcatcg ggcgcggtcg cggcgagcca gtacgcgatg aaaccaccga ggctgtggcc
     5701 gacgatgatc ggattcttca ggtggttgga tcggatgtag cggaccagct ctttgcgcgt
     5761 cttcgccacg agcggcggtt tgatcggctt ggtgccggcg aagcccgaca gcgtgatcac
     5821 gtgcgcctgg atctcgttgc cgagtcgcgc gacggtgtcg tcccagacct cgccggggca
     5881 ggcgaggcct gggatgaaga tcaccgggcg gccctgaccc gtcaccttcg cggtgaacgc
     5941 ggttggctgg aatccagcac ggttgacgaa gggaccgtcg gtttcgtcgt cggagcgcgg
     6001 ctgtgatttg ccgccacagg ccacgactgt gaccgtgagc gcaagcacga ccgcgacgag
     6061 ccgcatcgag ggagacgata cccctaggga gctgatacaa acgagtagat gcgcgcgttc
     6121 tggatgatcg gcctcgctct tgtcgcctgt ggtgattccg ataacgacgg cgatccgaag
     6181 caattcatcg atcagggcgg cgtgctcgcg atcgacggct gcaactacat gctgacgacg
     6241 cggatcggtg cggagcagcc ggtcccttcg ggcaagacga tcggcatgga accgacgccg
     6301 cgccaggtcc acctcggcat catgggcgat ccgaagacgt cgatcgtcgc gcagtggcgc
     6361 acggccgacg agacgacgac ggtcacgacg ttgcgctacg gcacgggcga gggcctgtcc
     6421 gaggcgcagc tcacgaacat cgtcaaaggc gtgcactttc gctacaactc gaccggcacc
     6481 gacctctacc ggatccatca ggcgcacgtc tgtggcctcg agcccggcac gacgtacagc
     6541 taccaggtcg gcgccgacaa gcactggtcg ccggtctaca cgttccacac cgcgcccgac
     6601 gtcgacgcgc atcccgacgc cgaggtcgtg ttggcctttg tcggcgacag ccgcggcggc
     6661 tacgacgtgt ggacgcagct ggtggaccag atcaagcagc gcacgcccga cctcgtgctg
     6721 ttctcgggcg acgcgatcac gatcggcctc acgcagtacg agtgggaaga gttcctgacc
     6781 gacggcgagc cactgttcgc gacgacgccg gtcgtgctca cgaacggcaa ccacgaggcg
     6841 aacgcgatca actacttctc gcagttcgcg atgccgggcg atcaggagaa cttcggcttc
     6901 gactacggct tcgcgcacgt gttcgtcgcg aacgactcgc cggataatcc gtcggacctc
     6961 acggacgtga ttccggtcgc gatggacgcc gacttcaccg accacacctc ggcgcgctgg
     7021 aagatgctga tgagccaccg gccgatgtac tcgtcaggca cccgacacgg ctccgcgatg
     7081 gatctgcaag ccgcgtgggg gccggtcgtc gacaagcaca aggtcgatct cgtgctcaac
     7141 ggccacgagc acatgttcga gatcagcaag ccgctgttca acaacgccgt acaggcgtcg
     7201 aacgccgatg gcaccgtgta cgtcgtcgcc ggtggcgcgg gcgccgagct ctacggcttc
     7261 ggcacgccgg gcttctggac cgattacatc gagtcagcgc acaccgcagc gacgatccgc
     7321 gtgcgccgcg atcagctcac gctcgattcg ttccgtcccg acggcaccgc ggtcccgaac
     7381 ggcttcacga agaccaagcc gtgaaactcg tcgcgctcgc agcactcctg ctgctggcgc
     7441 gcgtcgcggc tgcggacgac gctgacgccg aacgaacgtt ccgcgatgca tcgctgcgcg
     7501 gcgacatcac tgcactcgag cagctcggcg cacagcgccc gctgacgatc tggagcgacg
     7561 atgcgtgggc cgaggccgcg cgtctcgcca cacgagcgaa cgatttcacg cgggcacgcg
     7621 cggatctcga gcaggtgatc gcgattggca cagacgaccg actggtgcag cgcgccaaga
     7681 acgagctcgc gcgcatcacg agcttttccg gcagcgcggg cgagtggacg gctgtcgcta
     7741 ccgagcacga acggctcgcg ccgcagctca ccgccggcgg tgatccgaag ccggctcttc
     7801 gcgagctcga acagctcgtg cgtgcgcacc ccggctaccc gcgggcggcg atgctgatgg
     7861 tggcgatcgc gcagagctgg cagcgcgaag gcgagggcga acgtgcgatc ggctggctgc
     7921 acgaggcggc gcgcgttgcg accgagcgca cggacaagct gcggatcggc gccgagctgg
     7981 tgcgcgcgtt gattcgcgtc ggtgcgttcg ccgatgccca gcgcgagctc gccgcgctcg
     8041 acgcaggacc ggccctcgtg gcagcgctgc gcgccgagct gtcacgggcc gagttgcgcc
     8101 gcaccgtgcg ctggatcatg tggggcgtgc tggtcgcgct cgcggtcatc gcggcgatcg
     8161 cgctgcgccg agctgccggc tcgtggcgcg cggggttgcg ccggctcgcg cggccgccga
     8221 cggaggcgtt gtttctcgtg ccgattgcag cggtagtcgt cgggttttca ttcaccggca
     8281 atccgctggt cgcgcgcacg gtgctcgcga ttctcgcggc cggcgtcgcg acgagctggg
     8341 tatcgggcgc ggtgctcgag gggcagccgc gcgtgcgctt tcggcgcgca ctgctgcacg
     8401 catcgctcgc gatcgtcgcg gttgttgctg cggcatacct cgccgtcgat cgcggtcacg
     8461 tcatcgactt catcatcgag acgctgcgct cgggtcacga gcgcggctga ctactcgatg
     8521 aagtacggct ggatctgatc gaggtccgac cgcgcggtga acgttggctt gccgtcttcg
     8581 agcgcgaagc actgcgcggt gcggaacggc ggaaacgacg gccgccccat gatgtacgcg
     8641 ccgagtgagg agatcgacgg ctcgtgaccg acgacgagca ccgacatgcc cgcctgtgcg
     8701 atctggcctg cagcgatgcg cggcatcgcc gccggctcga gacaacgcca cgacgagatc
     8761 gcgccgatgt aatcgatgct ctgcgcgagg agctcggccg tctgcacggc gcgcggcaag
     8821 ggcgagcaca cgatgaggtc tggtgcgatg tgctgctcgc gcaacaggcg accgaggatg
     8881 cgcgcggctt cacgtccacg cggcgagagc caccggtcgc gatcgctacc cgcgtcttcc
     8941 tcggggaccg cgtcgccgtg cctcaccagg tagatgcgca tgccgcatcc tactgcggct
     9001 tcgtcaccgg cgcatcgctc ccttcctcgg gcgggagcgg tggttcgtcg ccgccggagc
     9061 cggtttcagc cgtcggcggc gcctgcttgc agagcggtgc gctggcgagc gacagcacgt
     9121 cgagcgcgag tccaggcttg ttctcgagcc gcagcgcctc gcggcccgtc gtcgcgtccc
     9181 agaggcggat cgcaccgtcg ccgtcttcgt acgcgagcgt gttcggatcg agccacgtcg
     9241 tcgcgaaccg gcttttcgcc gtcagcaaat gcttgagcgc cgcggtcttg ccgtttgcga
     9301 catagagcga cggtgcggtg tccttcgcgc acggatccac ggccgtcgcg aacgcgacgt
     9361 aaccgccgcc aggcgcaacg gcgaccgtct ccatcgccgc ttggcccgac tccggcactt
     9421 gcaccggtgg cacgcccttc gcgagctcga gcgtcggtgc gcgcggcgga tcgccgatcc
     9481 atgcggcgcc cacaccctcg atcgatttcg gcgggacgat gcgtccctcg tcgagtgtca
     9541 gctcgatgcg cggcgagccg agcggcgcgg tgaccttcgt cagcttgcca gtcgtgcgat
     9601 cgatcgaaga cacggtccag tcgccgatcg tccaccgccc agtcgccggt gccgtcgcga
     9661 cgagcagctg atcgccatcg ccgtagtaga gcgcagccgc gcgcgccgag ggcaggttga
     9721 tcttcggcga cgtgttcttc cactcggccg tgtcgaacgc ctgcacccac ccgcgcgcga
     9781 gcagcggcgg tgcgtcttct ttgctgcgat cgatcttgtc gtaaccgagg atcgcgacct
     9841 cgttgccctg tttcgcgcgc acgaagccgg cgacttgatc gcccgtgtgc gtgagccgca
     9901 agtagcgctt cgagtcgcga tcgaatgcgt acagctcgcc tttgctcagc cccgactgca
     9961 cgcctggctt gtccggccac ttgaacgagt tgcgccggcc gacgagccac aagccggctt
    10021 tgatctcccg cgcatattcg tcgtgaattt gcgccgcgag ctgggtgacc gcctggccgt
    10081 gctgcgtcgc gagaaagtcc ttgagctcgg gaatcgtcgc gagctcggcg ccgtacttgt
    10141 agtaatcggc cgcgattgca gcgacgaggt gatcgaccgc agccgctggc tcgccggtct
    10201 gcacgagcgc gatcgcgagc actctgtgcg cgtcgagatg attcggatct ttcgcgagcg
    10261 cagcttccgc ttgcttgcgc gcttccgccc agtccttctt cgcggcggca tcagcagcct
    10321 ttttgtacgc cgctgcgcct tggtcgtaga tgaacgagaa ccgtgtgatc ttgtcgaccc
    10381 cgagcactgg cagcgaaatg ggtgcggcta caggtgccga tgcagcatct ttcttcccgt
    10441 tcttgttgcc gcccttaccg caggaagcaa agaggaggat cacgagcgcg atgcgtgata
    10501 atggagtcag cactacctgg aggatgccat gaagatcatg atgatcgctt tcgtgttggc
    10561 cgcagtcgtc ggctgttcga aagacaagaa ggtcgcgaag cccaccccga cgaccgcaaa
    10621 gccggttgct gcgaagccgg cggatgacaa gctgctcgag acgctgcccg ccgaccagca
    10681 ggtcagcccg aatttgtctc tgtcgggcga catcgtgcag atgtgcggca tcaaggccac
    10741 ggccaatgcg accgcagcgc cgacgtttga ctacgacaag gaagagctgc accccgagga
    10801 ccgcgcggtg ctcgaccagc tcgcgacctg cctcatgacc ggcgcgctca agggccgcag
    10861 cgtcgcgctg accggccgcg ccgatccgcg cggcaccgag gagtacaacc tcggcctcgg
    10921 ctcgcgccgc gccgcgagcg tgagcacgta cctcgtgcgc ctcggtgtcg gtgagcctca
    10981 gctcggcgtc accacgcgcg gctcgctcga tgcgagcggc accgacgagg ccggctgggc
    11041 gaaagatcgc cgcgtcgata tcacgctcgc gaactagagc gcggccgcgg cgcggaacgc
    11101 ctcgatcgcc ttgatcgtct tgggccgcgt gatcggaccg cggccctgga cgaagaagac
    11161 gctatctccg tcggcgacgg cgaagatttc gtcgtagccc tgcacccgct ccggcggttg
    11221 cgcaggcggc ggctcggctt tcgcctcttg cgcgagctgc atcaggtgct gctcgttcgg
    11281 aggcgtgagc tcgcgtgtgc gctcctcggc gagcgcaccc gtcaacggcg tcgcggctgg
    11341 tgccttgccg gtgtaaatcg tcttcgcatc ggtatcgacg acgatgcggt agaccggcga
    11401 cttctcgccg ccgccggtca cgaacacgcc gtgcttgggg agcaacttct ggtactcggc
    11461 ggggacctcg gcgcgcgaag tgatgacgac ttccgcgcgg cggatgcgcg gctcttgagg
    11521 ggccgcacac gcgacgaaga ccaacacgac gacaacccgg gcgagcatgc ccgccactgt
    11581 atcctgcatg ctagtgtttc cgcgatggcg ctggagcacg tcgcacaagg gcaaacgcgg
    11641 ttcttcacgc ccggcgttgc ccccgatccg cgcggcatct tgctcggccg gttctgcctg
    11701 atgacgttcc cgacgctcga gggcgcggtg agctggctgc gtctgtattc ggccgaggca
    11761 tcgctcgacg agctgatggc aaacctcacg ctgacgaagg cgaagacggc actcggcagc
    11821 cgcgagatcg tgatccagat tcccgccgtg tcgtcgtacg cggccgatcg ctcggcgcgg
    11881 ctcgcgcggc tcgtcggtgg cgcgatctac accggcacgt cgaagcactt cgtcaaatac
    11941 cgcgatgatc ggtcgccgta cggctacgac gctgtcgaca tcggcgcgat gccggcgtcg
    12001 accgacttca tggtgcacgg cgacgagttc gcgcagggct acgtgcgcga gggcgagctg
    12061 ccgttcgcaa agctgctgtt ccggctgtcg atccggcgga ttccgggcgc tgacaagctg
    12121 gctaccgaag atcgcggcga gctctacctc gcggtcgcgc acggcctctc ggaaggcatc
    12181 attcgctacc tctggcgcaa ccgcgtcgag gcgcaggccg gcctgttcac gccgcgctcg
    12241 tcgagcgcct tcgacgaaca gcggcgcggc tacatgctga tccgcgcgcg tgcgttgccc
    12301 gagcgcatcc tggcgctatt cctcggcaca cctggcatcg atgtgttcaa gcccgcgggt
    12361 gcgaacgcgg cggtcgcggt tggctaccag catccgatcg atctcgcgtc gtgctcgtcg
    12421 gtgtttccgc ccgacacgtt ccacgtgttc tggccgaacg atcgcgtcga cgtgctgccc
    12481 ggtccgctca cgctcagcga catcgccgat ctcacgcgcg tcgacctcga gctcgacaag
    12541 ccacgcgatc cggcacagca cggcggtggc gcggccgagc cgatcggcgt cgagctgcgg
    12601 ctcgcggccg cgatggggcc gccgaagcgc gtcgtcgcga cgttgatcga gccgaagcat
    12661 gcgccgcggc tcaagcgcat cgtgtttgcg ctgccgaacg tgtcgctgcg cggccaacgc
    12721 gtcgcggtca ccgatcgcgg catactcatc atcggctcgg agaacctcga cgtcatcccg
    12781 ctcggccagc tgctctacga gctcacgccc ggtttgctcg ttccgctcgg gatggacatc
    12841 gtgccgcgcg tgacgcccga ggtgctcgcg cgcgcgctcg gtcaccaagg tggcgtcgtc
    12901 accgtgttca cgagcgacaa ccggccgttc caggtacccg actccgcgtt cacgtcgctc
    12961 gagcggcgat cgctcgcgaa gatggacgtc gaggcagcgg agacggtcga gctcggcgcg
    13021 gagccgagcg ccgaagcg
//
LOCUS       DASUFX010000379         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1728881, whole genome shotgun sequence.
ACCESSION   DASUFX010000379 DASUFX010000000
VERSION     DASUFX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728881"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFV99_15175"
     CDS             complement(<1..191)
                     /locus_tag="VFV99_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060709.1"
                     /translation="MKRAIASILLCLPVTAAAQPADGAEPAKSDKPEQEKPAAPDPAT
                     TAAAAPEPTKLVDKDVEPPT"
     gene            complement(216..1016)
                     /locus_tag="VFV99_15180"
     CDS             complement(216..1016)
                     /locus_tag="VFV99_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060710.1"
                     /translation="MKYFELEWRGGATATRLHKRRTGIEHLPWGTLATTDLDPAYRFE
                     ARAIWSNGVFTEYASAAAFSALTTAMLECGAPVDLIAMCGDIVVDEMFHVELSSRLTM
                     ELGGAMALEFDLANVAPAPSTQRPLMKAAELAVVTSCVSESLSVPAMARSRVLASEPL
                     CRAVLDRLLVDEGPHSRLGYWFLDWAAERLTDIERAALAATAVTTIEAYASLWQESCD
                     GCPPPLGLGGHDAVGRQDLRAAVENMIARPLARYGIELDKERVAQLVA"
     gene            complement(1013..1621)
                     /locus_tag="VFV99_15185"
     CDS             complement(1013..1621)
                     /locus_tag="VFV99_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060711.1"
                     /translation="MKLFAVAVLASASSAQAGTEAQSTQAARASTPANSGIDWNGTIE
                     VTAIRPRVLADGRPAAGNSVGKPLEQEAREDIDRTREVLLTASLAYQAAEQEPVIQPR
                     LLASYRPACGNTGGKSDPPADCTKAEIRAVEAYNKASADAYGKHYAKVQTAYDKLMIA
                     TASAVRADPTLKARLYVSGRPACGNTMSKKGPPAYCRTEAKL"
     gene            1721..2122
                     /locus_tag="VFV99_15190"
     CDS             1721..2122
                     /locus_tag="VFV99_15190"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX5060712.1"
                     /translation="MTAPGPIVLYDGECGLCHRSVKFLIKRDRHQLWYAPLQGETAAA
                     LRARHPEIPTTLESVVLVDHDRVYLRSKAFLYVAKYLTAPWRWAYYLRWLPAWLLDLG
                     YRVIARIRYRVWGKYDTCQLPTTDERAQLLP"
     gene            complement(2123..3226)
                     /locus_tag="VFV99_15195"
     CDS             complement(2123..3226)
                     /locus_tag="VFV99_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060713.1"
                     /translation="MRSLLIIVVALAIAGGRANAYPQFQLSKDQTCTSCHLSPSGGGL
                     LNENGANVAEAMSMFGQNPAFMYGAIETPDWLSLGGDLRGQWGYLQAPQRYLIGFPMQ
                     LDAYAAATKNNFSLHATVGYRTHQEGNEALTTVWARELYGMWQSEPGSSYGLFARVGQ
                     FMPVFGLRWVEHPLYVRRYGGTPLFSETPAVSVSYITGQYEVHATGFTKNPLYDPVRL
                     SSGGALYGEYRLGEHTQIGAGGMYEHQDWEYKLRGELTAKQYLPGPDLLLQAEVQLIN
                     PHVSGYGYRGLASVLMATYFMPKGVMIDAAWQHYDENLRIKDLDRDGFDLNVHWFATS
                     HIELILANQVEFMAFGKGGPTGAYSFLQLHYRL"
     gene            complement(3223..3783)
                     /locus_tag="VFV99_15200"
     CDS             complement(3223..3783)
                     /locus_tag="VFV99_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060714.1"
                     /translation="MHRIVWAPLLVLAACGETTDDRPLELDYLTQAIFAPTCGATQCH
                     SGFTQQGEDVFDTPEATRASLVDNGLIQFNSTKYDPDSPGKADLIIWITETVPFGGAT
                     VGEDGKPIGRMPYDAPMPNKDVLLLKEWIAAQAPGAQCNPDANKGTPAEGKACNNKEV
                     VECTPDWNFGPRVQLCSGDCVAGTCL"
     gene            complement(3899..4426)
                     /locus_tag="VFV99_15205"
     CDS             complement(3899..4426)
                     /locus_tag="VFV99_15205"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX5060715.1"
                     /translation="MAGYAGMPLPKKLGIKAGHKVCLLNAPGPFVRRLATEDVRIAHD
                     LRLPPVDVVVLFVDRVADLERRFADIANRLHPAGGFWVAFPKARRGCDITEEVVRRIA
                     LACGMVTNKVCSMGDFAGIRLVLRAEHRDAVAYRVEPPPVSRRMRRPTAAARMMHRPS
                     GAGSMMRRARARSTK"
     gene            4662..>5248
                     /gene="hemH"
                     /locus_tag="VFV99_15210"
     CDS             4662..>5248
                     /gene="hemH"
                     /locus_tag="VFV99_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5060716.1"
                     /translation="MPTGLLLINLGTPDAPTTPAVRRYLREFLSDPRVIDINPVGRAL
                     LLHLIILPTRPAKSAEAYRKVWDDQRGSPLLAFSKELAAGVQAKLGDSWRVALGMRYG
                     NPSIESALEALRGAGVDRIVVLPLYPQYAASSTATSVARVMELVSARWDVVPVDFVPA
                     FYADPGFLNAFAAVAKPALASARPDHVLFSFHGLP"
BASE COUNT          844 a         1813 c         1654 g          937 t
ORIGIN      
        1 gtcggcggct cgacgtcttt atcgacgagc ttcgtcggct ccggggcagc ggccgccgta
       61 gtcgcaggat ccggcgcggc cggcttctcc tgctccggct tgtcagactt cgctggctcg
      121 gcgccatccg cgggttgtgc cgcggcggtg acgggcaggc acaacaggat cgaagctatc
      181 gcgcgcttca ctacccggta cggtaacccg aaacgtcacg cgacaagctg tgcgacgcgc
      241 tccttgtcga gctcgatgcc gtagcgcgcg agcggccgcg cgatcatgtt ctcgaccgcc
      301 gcgcgcagat cttgcctgcc aaccgcatcg tgaccgccga gcccgagcgg aggcggacaa
      361 ccgtcacacg actcctgcca cagcgatgca tacgcttcga tcgtcgtgac cgccgtcgcg
      421 gcaagcgcgg cgcgctcgat atcggtcagc cgctccgccg cccagtcgag aaaccagtaa
      481 ccgagccgcg aatgcgggcc ttcgtcgacg agcaaacgat cgagcaccgc gcgacacagc
      541 ggctcgctcg cgagcacgcg cgaccgcgcc atcgccggca cgctcagcga ttcgctgacg
      601 cacgacgtga caacggcgag ctctgccgcc ttcatcagcg gccgctgcgt gctcggcgcg
      661 ggtgcaacgt tcgcgagatc gaactcgagt gccatcgcgc caccgagctc catcgtcagc
      721 cgcgacgaca gctcgacatg aaacatctcg tcgacgacga tgtcgccgca catcgcgatc
      781 agatcgacgg gcgcaccgca ctcgagcatc gccgtcgtca gcgccgagaa cgccgccgcg
      841 ctcgcatact cggtgaacac gccgttgctc cagatcgccc gcgcttcgaa gcgatatgcc
      901 ggatcgagat ccgtcgtcgc gagcgtgccc cacggcaagt gctcgatgcc ggtgcgtcgc
      961 ttgtgcaggc gagttgcggt cgcaccgccg cgccactcga gctcgaagta cttcacagct
     1021 tcgcctccgt gcggcaatac gcaggcggtc ccttcttgct catcgtgttg ccacacgcag
     1081 gccggccgct gacgtagagc cgcgccttca gcgtcggatc ggcgcgcacc gccgatgcgg
     1141 tcgcgatcat gagcttgtcg tacgcggtct gcaccttcgc gtagtgcttg ccgtacgcgt
     1201 cggccgacgc cttgttgtag gcctcgacag cgcgaatctc tgctttcgtg caatcggccg
     1261 gcggatcgct cttgccgccg gtgttgccgc acgcggggcg atagctcgcg agcaagcgcg
     1321 gctggatcac cggctcttgc tctgccgctt gatatgcgag ggacgcggtc agcagtacct
     1381 ctcgcgtccg atcgatgtcc tcacgcgctt cctgctccag cggcttgccg acgctgttgc
     1441 ccgccgccgg gcggccatcg gcgagcacgc gcggccggat cgcagtgacc tcgatcgtgc
     1501 cgttccagtc gatgccgctg ttcgcgggcg tgctcgcgcg cgccgcctgg gtagactgtg
     1561 cctccgtgcc cgcctgtgcg ctcgatgcgc tcgccagcac ggcaactgca aacaatttca
     1621 tccctcgccg attcgccatg ggtgtctcct tcaacgagca agacgagcgt ccgcgacaac
     1681 cgttaacggc gctgtcaggt ccgcgtaaac tcgcgtcgtc atgactgctc ccggccccat
     1741 cgttctgtat gacggcgaat gtgggctgtg ccatcgctcg gtgaagttcc tcatcaagcg
     1801 cgatcgccat cagctctggt acgcgccgct gcaaggcgag accgcggccg cgctgcgcgc
     1861 gcggcatccc gagatcccga ccacgctcga gtcagtcgtg ctcgtcgatc acgatcgcgt
     1921 gtacctgcgc tcgaaagcgt ttctctacgt cgcgaagtac ctcaccgcgc cgtggcgctg
     1981 ggcgtactac ctgcgctggc tgccggcgtg gctgctcgat ctcggctatc gcgtgatcgc
     2041 gcgcattcgc tatcgcgtct ggggcaagta cgacacctgc cagctaccga cgaccgacga
     2101 gcgcgcgcag ctactgccct agctacaggc ggtagtgcag ctgcaagaac gagtaggcgc
     2161 ccgtcgggcc gcctttgccg aacgccatga actcgacctg attggcgagg atcagctcga
     2221 tgtggctcgt cgcgaaccag tgcacgttga ggtcgaaccc atcgcgatcg agatccttga
     2281 tgcgcaggtt ctcgtcgtag tgctgccagg cggcgtcgat catcacgccc ttcggcatga
     2341 agtacgtcgc catcagcacg ctcgcgagac cgcggtagcc gtagcccgag acgtgtgggt
     2401 tgatcagctg cacctcggcc tgcagcaaca ggtcgggacc aggcaggtac tgcttggcgg
     2461 tcaattcccc gcgcagcttg tattcccagt cctggtgctc gtacatgccg ccggcgccga
     2521 tctgcgtgtg ctcgccgagc cgatactcgc cgtagagcgc gccaccgctc gatagccgca
     2581 ccgggtcgta gagcgggttc ttcgtgaagc ccgtcgcgtg cacctcgtac tgccccgtga
     2641 tgtacgaaac cgacaccgcg ggcgtctccg agaacagcgg cgtgccgcca tagcgccgca
     2701 cgtacaacgg gtgctcgacc cagcgcagac cgaacaccgg catgaactga ccgacgcgcg
     2761 cgaacaggcc atagctcgag cccggctcgc tctgccacat gccgtagagc tcgcgcgccc
     2821 agactgtcgt gagcgcctcg ttgccttcct ggtgcgtgcg gtagccgacc gtcgcgtgca
     2881 gcgagaagtt gttcttcgtt gcggccgcgt aggcgtcgag ctgcatcgga aaaccgatca
     2941 agtagcgctg cggcgcctgc agatagcccc actgaccgcg caagtcgccg ccgagcgaga
     3001 gccagtccgg cgtctcgatc gcgccgtaca tgaacgcggg gttctggccg aacatcgaca
     3061 tcgcctcggc gacgttcgcg ccgttctcgt tgagcagacc gccgcccgac ggcgagaggt
     3121 gacagctcgt gcacgtctga tccttgctga gctggaactg cgggtacgcg ttcgcgcggc
     3181 cacctgcgat cgcgagcgcc acgacaataa tgaggaggga cctcataggc acgtccctgc
     3241 gacgcagtcg ccactgcaca actgaacgcg cggaccgaaa ttccagtccg gggtgcactc
     3301 gacgacttcc ttgttgttgc acgccttccc ttccgcgggc gtgcccttgt ttgcgtccgg
     3361 attgcactgc gcgcccggtg cctgcgctgc gatccattcc ttgagcagca gcacgtcctt
     3421 gttcggcatc ggcgcgtcgt atggcatgcg cccgattggc ttgccgtcct cgcccaccgt
     3481 tgcgccgccg aacggcaccg tctcggtgat ccagataatg aggtcggcct tgcccgggct
     3541 atcggggtcg tacttggtcg agttgaactg gatcagcccg ttgtccacca agctcgcgcg
     3601 cgtggcctcg ggcgtgtcga acacgtcctc gccctgttgc gtgaagcccg agtgacactg
     3661 cgtggcgccg cacgtcggtg cgaagatcgc ctgcgtcagg tagtcgagct cgagcggtcg
     3721 atcgtcagta gtttcgccgc acgctgctaa cacaagcagc ggcgcccaca cgattcgatg
     3781 catgcgcgga cattacatga aggccggaag atcaggcgac ttcgtacgtg ccacacagca
     3841 ttccacgtgc gcgccatgtg catgtccaca acacgcgctg caggcgccgc gacgcgcgtt
     3901 acttggtgga ccgagcgcga gcgcgacgca tcatcgagcc agcaccgctc ggacgatgca
     3961 tcatgcgcgc agcagcggtg ggccgacgca tgcggcgcga caccggcggc ggctcgacgc
     4021 gatacgcgac agcgtcgcga tgctcggcac gcagcacgag gcgaatgccg gcaaagtcgc
     4081 ccatcgagca gaccttgttc gtgaccatgc cgcacgcgag cgcgatgcga cgcaccacct
     4141 cttcggtgat gtcgcagccg cggcgcgctt tcgggaacgc aacccagaag ccaccggcag
     4201 gatgcaggcg gttcgcgatg tcggcgaagc gacgctcgag gtcagcaacg cgatcgacga
     4261 acagcacgac cacgtcgacc ggaggcagcc tcagatcgtg cgcgatgcgc acgtcttctg
     4321 tggcgagccg gcgcacgaat gggccagggg cgttgagcag acacaccttg tgccccgcct
     4381 tgatccccag cttcttcggt agcggcatac ccgcgtagcc agccatgccc acgcatcatg
     4441 cagcggctct ttccccggtc aaacaggatg gccaaatctt cttttaagtt ccggcaacga
     4501 ctaggtttcg gtgtgatcgc cgtactcccg cccattcgta gggacgatga cgaatcgccc
     4561 tgtggatagc gtgggtttcg gcctgattat gaaatcgatt actcccggcc ggccgtttcc
     4621 ttgactttgg tgcccgggtt cgtcagcaag gggttccgga cgtgccgact ggtcttcttc
     4681 tcatcaacct gggaacgccc gacgcgccga ccacgcctgc cgtgcgtcgc tacctgcgcg
     4741 agttcctctc cgacccgcgc gtcatcgaca tcaacccggt cggccgtgcg ctgttgctac
     4801 acctgatcat tctgccgacg cgtccggcga agagcgccga ggcgtatcgc aaagtctggg
     4861 atgaccagcg cggctcgccg ctgctcgcgt tttcgaaaga gctcgcggcc ggcgtgcaag
     4921 ccaagctcgg tgacagctgg cgggtcgcgc tcgggatgcg ctacggcaac ccgtcgatcg
     4981 agagcgcact cgaagcgctg cgcggcgcgg gcgtcgatcg gatcgtcgtg ctcccgctct
     5041 atccccagta cgccgcatcg tcgaccgcga cctcggttgc gcgggtgatg gagctggtga
     5101 gcgcgcgctg ggacgtcgtg ccggtcgact tcgtgcccgc gttctacgcc gacccggggt
     5161 tcttgaatgc gttcgccgcg gtcgccaagc ccgcgctcgc ctcggcgcgc cccgatcacg
     5221 tgctgttttc ctttcacggc ctccccga
//
LOCUS       DASUFX010000380         8870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1449081, whole genome shotgun sequence.
ACCESSION   DASUFX010000380 DASUFX010000000
VERSION     DASUFX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449081"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /gene="rpoN"
                     /locus_tag="VFV99_15215"
     CDS             <1..1396
                     /gene="rpoN"
                     /locus_tag="VFV99_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5060717.1"
                     /translation="ADLVHEEMLENPILDDELDLDNERSRERDEMGGDEQQLHQLEGA
                     GTTETPVEGSVVDGVAQTEIKGDAAAVSEIDWENYLDNYSMAPPMPAFRGDGEELPSL
                     EATLTKPPSLPDHLAWQLKMVNPPLTPEQLEIGLTIIGNIDADGYIKDPPLPELAADL
                     GVDETRVQEVLETIQTFDPTGVGARSLAECMLIQAMACGQDDDLVVKMIKSHLGNLEK
                     KNYQAIARDLKQPLEEIYEAAKVIMDFDPRPGRQYSLDDPHYVTPDVYIHKVGDKYFV
                     VPNDDGLPKLKISGFYKNAMGNSNASKDYVQDKLRSAQWLIRSIQQRQRTIIKVTESI
                     LKFQREFFDKGIAHLKPLILRDVAEDIGMHESTVSRVTTSKYVHTPQGIFELKFFFNS
                     GISRTNGEDLASQAVKSKIKELVAGEDPKRPHSDQKIVELLKKGGIDIARRTVAKYRE
                     QLGILSSSKRKQVF"
     gene            complement(1393..2208)
                     /locus_tag="VFV99_15220"
     CDS             complement(1393..2208)
                     /locus_tag="VFV99_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060718.1"
                     /translation="MKTCYSAFVFVFAGCVVNGKAYGPGSSTTNSSTTSGATTAEPSG
                     STSTSEAAATNGAPPQAGSPVNDQVSSNENAPYPDAPADPWAGVAGDQPKRRSDDRWT
                     VRTRDFACTAAHDHCLDTDAWFIVDMQPRPLSYQRNGEVYVFGKDEPFGAWNAVMKNH
                     GPDHYVAYRTVPATKQNLAPGVLVFGYKSDYPANGRKAVEDSWAHGKVASIDLELGYY
                     TLEGREQQLRLSGARVAVLRWEPGGKVEIIGGKRRDELAVRAADVFLPAPERD"
     gene            2408..2770
                     /locus_tag="VFV99_15225"
     CDS             2408..2770
                     /locus_tag="VFV99_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060719.1"
                     /translation="MHEDSPPPFIVIRTPKRPDVYDRTLELVALVQRILEGASARFHL
                     KDRLDRVVTSLVFELSRAKHGAKTTRWRHYRRAEELASDCATILDILVHQQAAALSDL
                     EPARKLVHELLAELNALG"
     gene            2865..3482
                     /gene="raiA"
                     /locus_tag="VFV99_15230"
     CDS             2865..3482
                     /gene="raiA"
                     /locus_tag="VFV99_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5060720.1"
                     /translation="MQFAVTFRHMEPTEALKSYARERMERVRKYLPDPIACHVVLSTE
                     RHNHRIDVAFQLHNGLSIAGHETTENMYSSIDLCIAKIERQVRKYKGKLEGMRARPHH
                     VVALPWSHSIIDEAFHELNEMNGNGHHKAVTKSDAPSSQPEFTVVRTEKFHAQPMSVS
                     DAIVQLNLLHEPFLVFRHDTDGRVAVVYKREDGSYGLIETPTGSA"
     gene            3545..4006
                     /locus_tag="VFV99_15235"
     CDS             3545..4006
                     /locus_tag="VFV99_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5060721.1"
                     /translation="MKIVDLIKRDMVVPALVATDKRGILEELAAYMSAHHSRIDRAAL
                     AKVLVEREQLASTAIGEGVAIPHGKLGAVGEIVACLGRAPTGVEFDSMDGQPTFLFFV
                     LVAPESSTGAHLKALARISRVFKDPEFRRRLLAASDAETMYHVVAEEDAKY"
     gene            4007..4996
                     /gene="hprK"
                     /locus_tag="VFV99_15240"
     CDS             4007..4996
                     /gene="hprK"
                     /locus_tag="VFV99_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX5060722.1"
                     /translation="MADGAPDDAVGGRPGRTVAISVAELLDRAELAVTIAAGKVGLGR
                     EVLVPRIQKPGLALTGWPEQLHANRVLVLGGTEIDYLKDHEPARRVGIETLLASEPAC
                     VVVCRSLVPPDELLAAGEARGVPVLVSSLVTADFIAAVTTWMADRLAPSTELHGVLMD
                     VLGIGVLMVGKSGIGKSETALDLVVRGHRLVGDDVIRIRRHGHQLSGRGAGILGHHME
                     IRGLGIINIKDLFGISAVREAKVIDLIVELHEWDAAVEYDRLGFDEHREKLLDVAITK
                     LRLPVRPGRNIATLIEVAARNQLLKHQGIHSARAFRDTLHRAMHDAEGADAVE"
     gene            4997..5830
                     /gene="rapZ"
                     /locus_tag="VFV99_15245"
     CDS             4997..5830
                     /gene="rapZ"
                     /locus_tag="VFV99_15245"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX5060723.1"
                     /translation="MQIVIVTGLSGAGKSTALRALEDIEFTCVDNIPVPLVTQMVDHL
                     AHEGDRDKLAIAIDARQRRALTAWQQTLATLRSSGHRLEVMFLDASDEVLLRRFSETR
                     RRHPLSGDDLIDGIRRDRELMAEMRQGAAVVDTSQLNTHQLKAIIQDRYGGTGKLAVT
                     IVSFGFKHGLPLEFNLVFDVRFLPNPYFEPSLTHLDGRDPDVAKFVLGPDGSELVRQV
                     ESLMRFSLPHFQKEGKLYVTVAVGCTGGRHRSVAVVEELRRRLAGEWDILVRHRDVER
                     E"
     gene            5827..6105
                     /locus_tag="VFV99_15250"
     CDS             5827..6105
                     /locus_tag="VFV99_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX5060724.1"
                     /translation="MSDFAEKTLLIQNELGLHARAATKLVQLATKFPCEITVTKDGHE
                     VNGKSIMGVLMLVASKGTTVTLKAKGDRAKEAVDAIAALIDDKFGEGK"
     gene            6108..7877
                     /gene="ptsP"
                     /locus_tag="VFV99_15255"
     CDS             6108..7877
                     /gene="ptsP"
                     /locus_tag="VFV99_15255"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX5060725.1"
                     /translation="MTVREEIRRTGLGVSPGIAFGRAYLIGRDTLKAPRHHIESDDVD
                     TEVARLYKAIASSDKQLAKIKEKLASENESDYHIITAHQMMLHDEHLVGAAVEYIRDD
                     KINAEWALRRAVDDIRGVFDQIEDSYLRERRSDVEFVFERVLRNLLGRDMGPLSPPPD
                     AIVVAYDLSPADTATLHKAAVAGLLTDAGGKTSHTAIIARAHEIPAVVALENITELVE
                     TDDLLLLDGSTGMVIVNPSASTVAQYREEQRRQVAEGVRFHAMRDLPARTRDEANVAL
                     LANIDGPDELEDAIEYGAMGVGLFRTEYLFMAGTDIPDEEQHYQTAVAVLDKLKGRPA
                     TIRTFDLGSDKLAKFIEEADLHEQNPALGLRSIRLCLSSLGRDLFRAQLRGLLRASAH
                     GPIKLMFPMISGVAELRAVKTVVDDVKRELDEEGKAYDKDIKLGIMIEMPSAAMTADL
                     LAAECDFFSIGTNDLIQYTMAVDRVNEYVSYLYEPLHPSLIRLIGEVARAANAANIPV
                     TVCGEMAGEPMIAPVLFGLGIRELSMSAVSIPEVKATIRQMTVTETQSLVERVRNLPT
                     AADVRAMVTDYVFGLATPRKEPV"
     gene            7946..>8870
                     /locus_tag="VFV99_15260"
     CDS             7946..>8870
                     /locus_tag="VFV99_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060726.1"
                     /translation="MFGLLAVLAGRAEAGDPHRVYRTVETEHFIVHYWVPLDDVARRI
                     GVVAERAHRVLSPALDHAPAGKTLIYLTDDTDSANGFASVLPRNAIQLYVTAPSGFTE
                     LDDYDDWLYGLVAHEYSHILHLDTMSGLPNIYNSIFGKTWAPNQIMPRWVIEGIATYE
                     ESKRTAGGRNRGTRFDEQIRIAWKGDYDLRLDQVSGAPRQYPRGNAAYIYGSHFLQYV
                     FDRFGDDTLREMSHTSGSYAPPFAVNRQIAKVVGKPFTELYDDWQQYLRDRYALQEMA
                     AARRGLVTGRPITQSHEINLYPFYTADGRELY"
BASE COUNT         1640 a         2888 c         2880 g         1462 t
ORIGIN      
        1 ggccgacctc gtgcatgagg agatgctcga gaaccccatt ctcgacgacg agctggacct
       61 cgacaacgag cgcagccgcg aacgagacga gatgggcggc gacgagcagc agctccatca
      121 gctcgagggc gccggcacga ccgagacccc ggtcgaaggc tccgtggtcg atggcgtcgc
      181 gcagaccgaa atcaaaggcg acgccgccgc cgtatcggag atcgactggg aaaactacct
      241 cgacaactac tcgatggccc cgccgatgcc cgcgttccgg ggcgatggcg aggagctacc
      301 gtcgctcgaa gcgacgctga ccaagccgcc gagcctgccg gaccacctgg cctggcagct
      361 caagatggtc aacccgccgc tgacgccgga gcagctcgag atcggcctga cgatcatcgg
      421 caacatcgac gccgacggtt acatcaagga cccgccgctc cccgagctgg ccgctgacct
      481 cggcgtcgac gagacgcgcg tccaggaagt cctcgagacg atccagacgt tcgatccgac
      541 tggcgtcggc gcgcgcagtc ttgccgagtg catgctgatc caagcgatgg cgtgcggcca
      601 ggacgacgac ctcgtcgtca agatgatcaa gagccacctc ggcaacctcg agaagaagaa
      661 ctaccaggcg atcgcgcgcg atctgaagca gccgctcgaa gagatctacg aggccgcgaa
      721 ggtcatcatg gatttcgatc cgcggcccgg tcgccagtac tcgctcgacg atccgcacta
      781 cgtcacgccc gacgtctaca tccacaaggt cggcgacaag tacttcgtcg tgccgaacga
      841 tgacggtctg ccgaagctga agatcagcgg cttctacaag aacgcgatgg gcaactcgaa
      901 cgcgagcaag gactacgtcc aggacaagct ccgctcggcg cagtggctga tccgttcgat
      961 ccagcagcgc cagcgcacga tcatcaaggt caccgaatcg atcctgaagt tccagcgcga
     1021 gttcttcgac aagggcatcg cgcacctcaa gccgctcatt ctgcgtgacg tcgccgagga
     1081 catcggcatg cacgagtcga cggtttcgcg cgtgaccacg tcgaagtacg tgcacacgcc
     1141 gcaaggcatc ttcgagctga aattcttctt caactcgggc atctcgcgca cgaacggcga
     1201 ggacctcgcg tcgcaagccg tgaagtcgaa gatcaaagag ctcgtcgccg gcgaggaccc
     1261 caagcgcccg cactcggatc agaagatcgt cgagctgctc aagaagggcg gcatcgacat
     1321 cgctcgccgt acggtcgcga agtaccgcga gcagctcggc atcctgtcga gcagcaagcg
     1381 caagcaagtc ttctagtccc gctcgggcgc gggcaggaac acgtcagccg cacgcacggc
     1441 cagctcgtcg cggcgcttgc cgccgatgat ctcgaccttg ccgccgggtt cccagcgcag
     1501 cacggcaacg cgcgcaccgg atagtcgcag ctgctgctcg cggccctcga gcgtgtagta
     1561 gccaagctcg aggtcgatgc tggcgacctt gccgtgcgcc cagctgtcct cgactgcttt
     1621 gcgaccgttc gccggatagt cggacttgta gccgaacacc agtacaccag gcgccaggtt
     1681 ctgcttggtc gccggcaccg tgcgataggc gacgtagtga tcgggaccgt ggtttttcat
     1741 gactgcgttc cacgcgccga acggctcgtc cttgccgaac acgtagactt cgccgttgcg
     1801 ctgatacgac agcggccgtg gctgcatatc gacgatgaac caggcatcgg tgtcgagaca
     1861 gtgatcgtgc gcagcagtgc aagcgaagtc gcgcgtgcgc acggtccaac gatcgtcaga
     1921 gcggcgcttg ggttgatcgc ccgcgacacc agcccacgga tctgcgggcg cgtcggggta
     1981 gggcgcgttc tcgttgctcg acacctggtc gttcaccggc gagcccgctt ggggcggtgc
     2041 tccgttcgtc gcggccgctt ccgacgtgga cgtcgagcca gatggctccg cggtcgtcgc
     2101 gccgctggtc gtcgagctgt tcgtcgtcga gctgcccggt ccgtacgctt tgccgttcac
     2161 cacacagccc gcgaacacga acacgaatgc gctgtagcaa gtcttcattt ctgcctccaa
     2221 tggtgcgtct ttcgtccgcc gccggccgcg gttgcggatc gcacgcaact gcccgcgcgg
     2281 accgtcgatc cgcccctcaa aaggagatgc ggtcggctcg ttggcggcgc gcccgcgaag
     2341 tagtcggtcg ggtagggccg gggaggctaa ccgaaacagc ggcgggttcc gtgcgtacta
     2401 aacgtacatg catgaggact cgccgccgcc gttcatcgtc atccgcacgc cgaagcgccc
     2461 tgacgtatat gatcgtacgc tcgagctggt cgcgctcgtc cagcgcatcc tcgaaggcgc
     2521 tagcgcacgg ttccacttga aggacaggtt ggaccgggtg gtcacgtcgc tcgtgttcga
     2581 gctgtcccgc gccaagcatg gcgcgaagac cacacgctgg cgtcactatc gccgtgccga
     2641 ggagctagcg agcgattgcg ccacgatcct cgacatcttg gtccaccagc aggcggcagc
     2701 cctcagcgat ctggagccgg cgcgcaagct cgtgcacgag ttgctcgccg agctgaacgc
     2761 tctcgggtag aatctcctcc ccttcaagaa ttctcgtcga ggatctggct gatcatccga
     2821 gagggcgggg gtatcatcga agcgaaccat tagggaggcg ctcgatgcaa ttcgcggtga
     2881 cgttccgcca catggaaccg accgaagctt tgaagtctta cgcacgagag cgaatggaac
     2941 gagtcagaaa gtacctacca gacccaatcg cctgtcacgt agtactcagc accgaacgtc
     3001 ataatcaccg catcgatgtc gctttccagc tccacaacgg gctgtcgata gccggtcacg
     3061 agacgaccga gaacatgtac agctcgatcg atctttgcat cgcgaagatc gagcgccagg
     3121 tccgcaagta caagggcaag ctcgaaggca tgcgtgcgcg cccgcaccat gttgtcgcct
     3181 tgccgtggtc tcactcgatc atcgacgagg cctttcacga gctgaacgaa atgaacggga
     3241 acggccatca caaggccgtc accaaatcgg acgcgccgag ctcgcagccg gagttcaccg
     3301 tcgtgcggac cgagaagttc cacgcgcagc cgatgtccgt ctcggatgcg atcgtgcagc
     3361 tgaacctgct gcatgagcct ttcctcgtat tccggcatga cacggatggc cgagtggcag
     3421 tcgtgtacaa gcgtgaggac ggtagctacg gtctgatcga gacgccgacc ggatccgcat
     3481 aatcaatcac cctcggggcg agcgccgacc cgccataaac agtggtaggg tcttcagcgt
     3541 agtaatgaag atcgtcgacc tgatcaagcg cgacatggtc gtccccgcgc tcgtcgcgac
     3601 cgacaagcgc ggcattctcg aggagcttgc cgcgtacatg tccgcgcacc actcgcggat
     3661 cgatcgcgct gcgctcgcga aggtcttggt cgaacgcgag cagctcgcgt cgaccgcgat
     3721 cggtgaaggg gtcgcgattc cacacggcaa gctcggcgcg gtcggcgaga tcgtggcgtg
     3781 cctgggccgc gcgccgaccg gggtcgagtt cgactcgatg gacggtcagc cgacgttctt
     3841 gttcttcgtg ctggtcgctc ccgagagctc gaccggcgca cacctcaagg cgctcgcgcg
     3901 gatcagccgc gtgttcaaag accccgagtt ccgccgccgg ctcctggccg cgtcggatgc
     3961 cgagacgatg taccacgtgg ttgccgaaga ggacgccaag tactagatgg ccgacggcgc
     4021 acccgacgac gcagtcggcg gccgacccgg ccgaacagtg gcgatctccg tcgcggagct
     4081 gctcgaccga gcggagcttg ccgtgaccat cgcggcaggc aaggtggggc tcggccgcga
     4141 ggtcctcgtg ccgcggatcc agaagcccgg cctcgcgctc accggctggc cggagcagct
     4201 gcacgcgaat cgcgtcctcg tgctcggcgg caccgagatc gattacctga aggatcacga
     4261 acccgcgcgc cgcgtcggta tcgagacgtt gctcgcgagc gagccggcct gcgtcgtcgt
     4321 gtgccggtcg ctggtcccgc cggacgagct gctcgccgcc ggcgaggcga ggggcgtgcc
     4381 ggtgctcgtg tcttcgctcg tcaccgcgga cttcatcgcg gcggtgacga cgtggatggc
     4441 cgaccgactc gcgccctcga ccgagctgca cggcgtcttg atggacgtgc tcggaatcgg
     4501 cgtcttgatg gtcggcaaga gcggcatcgg caagagcgaa accgcgctcg acctcgtggt
     4561 gcgtggccac cggctcgtcg gtgacgacgt gatccggatc cgccgccatg gtcatcagct
     4621 ctcggggcgg ggcgccggga tcctcgggca ccacatggag atccgcggcc tgggaatcat
     4681 caacatcaaa gacctgttcg gtatctcggc tgtgcgggaa gccaaggtca tcgatctcat
     4741 cgtcgagctg cacgagtggg atgctgcggt cgagtacgac cggctcggct tcgacgagca
     4801 ccgcgagaag ttactagacg ttgcgatcac gaagctgcgt ttgccggtgc ggcccgggcg
     4861 caacatcgct acgctgatcg aagttgccgc acgcaaccag ttactgaagc accaaggaat
     4921 ccactcggcg cgcgcatttc gcgacacgct gcatcgcgcg atgcacgacg ccgagggtgc
     4981 ggacgcggtg gagtagatgc agatcgtcat cgtcacgggg ctatcgggtg ccggaaagag
     5041 cacggcgttg cgtgctctcg aggacatcga gttcacgtgc gtcgacaaca tcccggttcc
     5101 gctcgtcacg cagatggtcg atcacctcgc gcacgagggt gatcgcgaca agctcgcgat
     5161 cgcgatcgat gcgcgccagc gtcgcgcact caccgcgtgg caacagacgc tcgcgacact
     5221 gcgcagctcg gggcatcgcc tcgaggtgat gttcctcgac gcgagcgacg aggtcctgct
     5281 gcgccgattc tccgagacgc gccgccggca tccgctgtcc ggcgacgatc tgatcgatgg
     5341 catccgccgc gatcgcgaat tgatggcgga gatgcgccag ggcgctgccg tcgtcgatac
     5401 gtcgcagctc aacacgcatc agctgaaggc gatcatccag gatcgctacg gcggtaccgg
     5461 caagctcgcg gtgacgatcg tgtcgttcgg cttcaagcac ggcctgccgc tcgagttcaa
     5521 cctcgtgttc gacgtgcggt tcttgccgaa cccgtacttc gagccgtcgc tcacgcatct
     5581 cgacgggcgc gatccggatg tcgcgaagtt cgtgctcggt ccggacggaa gcgagctcgt
     5641 gcgccaggtc gagagcctga tgcgcttctc gctcccgcac ttccagaaag agggcaagct
     5701 ctacgtcacc gtggcagtcg gctgcaccgg cggccgtcat cgctcggtcg cggtcgtcga
     5761 agagctgcgc cgccggctcg ctggtgaatg ggacatcctc gtgcgtcatc gcgacgtgga
     5821 gcgcgaatga gtgacttcgc cgagaagacg ctgctgatcc agaacgagct gggtctgcac
     5881 gcgcgtgcgg cgaccaagct cgtccagctg gcgaccaagt ttccgtgcga gatcacggtg
     5941 acgaaggatg gccacgaggt caacggcaag agcatcatgg gtgtcctgat gctggtcgcg
     6001 agtaaaggca cgacggtcac gctcaaggcg aagggcgacc gcgcgaagga agcggtcgat
     6061 gcgatcgcgg ccttgatcga cgacaagttc ggcgagggca agtagccgtg accgtgcgcg
     6121 aggagatccg gcgaacgggt ctcggtgtct caccgggcat cgccttcggc cgcgcgtatt
     6181 tgatcggtcg cgacacgctc aaggcgccgc gccatcacat cgagagcgac gacgtcgata
     6241 ccgaggtcgc gcgcctctac aaggcgatcg cgtcgagcga caagcagctc gcgaagatca
     6301 aagagaagct cgcgagcgaa aacgagagtg actaccacat catcaccgcg caccagatga
     6361 tgctccacga cgagcacctc gtcggtgcgg ccgtcgagta catccgcgat gacaagatca
     6421 acgcggagtg ggcgctgcgc cgcgccgtcg acgacatccg cggcgtcttc gatcagatcg
     6481 aggactcgta tctgcgcgag cggcgcagcg acgtcgagtt cgtgttcgaa cgcgtgctgc
     6541 gcaacttgct cggccgcgac atgggtccgc tgtcgccgcc gcccgacgcg atcgtcgtcg
     6601 cgtacgacct gtcgccggcc gacaccgcga cgctgcacaa ggcggcggtc gccggtctgc
     6661 tcacggacgc tggcggcaag acgagccaca ccgcgatcat cgcgcgcgcg cacgaaatcc
     6721 cggcggtcgt cgcactcgag aacatcaccg agctcgtcga gaccgacgac ctgctgctgc
     6781 tcgacggcag caccggcatg gtgatcgtca atccatcggc gagcaccgtc gcgcaatatc
     6841 gcgaggagca gcgccgccag gtcgccgagg gcgtgcgctt ccacgcgatg cgcgatctac
     6901 cggcgcgcac gcgcgacgaa gccaacgtcg cgctgctggc aaacatcgac ggcccggacg
     6961 agctcgagga tgcgatcgag tacggcgcga tgggcgtcgg gctgttccgc accgaatatc
     7021 tgttcatggc ggggaccgac atccccgacg aggagcagca ctatcagact gcggtcgccg
     7081 tgctcgacaa gctgaagggc aggccggcga cgatccgcac gttcgacctc ggcagcgaca
     7141 agctcgccaa gttcatcgag gaggcggacc tccacgagca gaacccggcg ctcggcctgc
     7201 gctcgatccg gctgtgcctg tcgtcgctcg gccgcgacct gttccgcgcc cagctgcgcg
     7261 gcctgctgcg tgcctccgcg cacggaccga tcaagctgat gttcccgatg atctcgggtg
     7321 tcgccgagct gcgcgcggtg aagaccgtcg tcgacgacgt caagcgcgag ctcgacgaag
     7381 agggcaaggc gtacgacaag gacatcaagc tcggcatcat gatcgagatg ccgagtgccg
     7441 cgatgacggc cgacctgctc gcggccgagt gcgacttctt ttcgatcggc acgaacgacc
     7501 tcatccagta cacgatggca gtcgatcgcg tgaacgagta cgtgtcgtac ttgtacgagc
     7561 cgctgcatcc gtcgctgatc cgattgatcg gcgaggtcgc gcgcgcggcg aacgcggcga
     7621 acattccggt cacggtgtgt ggcgagatgg ccggcgagcc gatgatcgcg ccggtgctgt
     7681 tcggcctcgg cattcgcgag ctgtcgatga gcgcggtgtc gattcccgag gtgaaggcga
     7741 cgatccgcca gatgacggtc accgagacgc agagcctcgt cgagcgcgtc cgcaacctgc
     7801 cgaccgccgc cgacgtgcgt gcgatggtca ccgactacgt gttcggcctc gcgacgccgc
     7861 gtaaggagcc tgtgtgacat caggcttcgg gcctcgggct tcaggcttcg ggttcggagg
     7921 gtgggggttc agcgtaaggc tggtggtgtt cgggttgcta gcggtgttag cgggaagagc
     7981 ggaggcgggc gatccgcacc gcgtctatcg caccgtcgag accgagcact tcatcgtgca
     8041 ctactgggtg ccgctcgacg acgtcgcgcg gcgcattggc gtggtcgccg agcgagctca
     8101 tcgcgtgctg tctcccgcgc tcgatcatgc gccggcgggc aagacgctga tctacctcac
     8161 cgacgacacc gacagcgcga acggcttcgc gagcgtgctg ccgcgcaacg cgatccagct
     8221 ctacgtgacc gcaccttcgg gattcaccga gctcgacgac tacgacgact ggctctacgg
     8281 cctcgtcgcg cacgagtact cgcacatcct ccatctcgac acgatgtcgg ggctgccgaa
     8341 catctacaac tcgatcttcg gcaagacgtg ggcgccgaac cagatcatgc cgcgctgggt
     8401 gatcgagggc atcgcgacct acgaagaatc gaagcgcacc gcgggcggtc gcaatcgcgg
     8461 cacgcgcttc gacgagcaga tccgcatcgc gtggaagggc gactacgacc tgcggctcga
     8521 tcaggtgagc ggcgcgccgc gccagtatcc gcgtggcaac gcggcataca tctacggttc
     8581 gcacttcctc cagtacgtgt tcgatcgctt cggtgacgac acgctgcgcg agatgtctca
     8641 cacgtcgggt tcgtacgcgc cgccgttcgc ggtcaaccgc cagatcgcga aggtcgtcgg
     8701 caagccgttc accgaacttt acgacgactg gcagcagtac ctgcgcgatc gctacgcgct
     8761 ccaggaaatg gctgccgcgc ggcgaggcct cgtcaccggc cggccgatca cgcagagcca
     8821 cgagatcaac ctttatccgt tctacacggc cgacggacgc gagctgtact
//
LOCUS       DASUFX010000381         6887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_616205,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000381 DASUFX010000000
VERSION     DASUFX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616205"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..739
                     /locus_tag="VFV99_15265"
     CDS             50..739
                     /locus_tag="VFV99_15265"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060727.1"
                     /translation="MKSLQPDPLQGSFTLRGAKVVVLGYDEDAREHALALRRANNSVL
                     IGVRPDTTCWMRACADGFAADRPSAIVGSAEVVVMLVREPMATWAKVEPLLAPGALVV
                     FGNARLLEAMTYSRGGFDSVLVTTVDDAHTGCRVAVHRDATGKALLRAVGYARAACGI
                     DVALRATSVEAEADLELASHGERAGSLLALAASCECLPVAKPQAMIVDDDEDEKEPSW
                     LDSMLAWRSRM"
     gene            721..2448
                     /gene="ilvB"
                     /locus_tag="VFV99_15270"
     CDS             721..2448
                     /gene="ilvB"
                     /locus_tag="VFV99_15270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX5060728.1"
                     /translation="MAEPHVTTRTGAQILWDALHREGVDVVFGYPGGAIMPAYDALLD
                     SPIRHVLVRHEQGAAHMADGSARVSGKVGVAVATSGPGATNLVTGIATAMLDSVPLVC
                     ITGQVASRFIGSDAFQETDITGVTLPITKHNYLVTRPQDIAKTIREAFYVARSGRPGP
                     VLVDITKDAQQMSCEPVFSDEPVRMRGYRPDHRAGLDDLKRAAELIDRAKRPVILAGH
                     GIVKAEATTLLRALVVKTGMPVASTLLGLGGFPATHPLSLGMMGMHGEAWVNQAIQEA
                     DLLVALGMRFDDRVTGKLDTYAVNAKKIHCELDPAEINKIVRADVALVGDVRETLEAL
                     LPLVEARDHHDWVGRISELKGDSAVRDIQQMPPAGKLYAAHVMHDLWRITEGKALVVT
                     DVGQHQMWEAQYYKHDHPRKLVTSGGLGTMGFALPAALGARFAAPDDEIWVIAGDGGF
                     QMTACELSTIAQEGVKLNIAIINNGYLGMVRQWQQFFYNNRYSQTPLKSPDFVKLAEA
                     HGLFGLRVQSREEVAPAVMRARAARGTVLIDFRVEQEDSVYPMVPAGAALHDMIRRPS
                     PIVETAEDD"
     gene            2451..2990
                     /gene="ilvN"
                     /locus_tag="VFV99_15275"
     CDS             2451..2990
                     /gene="ilvN"
                     /locus_tag="VFV99_15275"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5060729.1"
                     /translation="MNIRLRTFIMHVEDLPGVLNRVTSLFRRRGYNIESLAVGRTEVA
                     GVSRMTIVMEADDDAARRIEANLYKLVNVLRVEDTTWRPTVSRELALIKVRTNEANRA
                     QVLQLTEVFRARVIDVSPEAVIFEITGTRDKVDSLAEVLAPYGVVELVRTGAIAMVRS
                     AEALEATPTVSEQPQTNAA"
     gene            3003..4016
                     /gene="ilvC"
                     /locus_tag="VFV99_15280"
     CDS             3003..4016
                     /gene="ilvC"
                     /locus_tag="VFV99_15280"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5060730.1"
                     /translation="MATIYYDKDADLSLIRGKRVAIVGYGSQGHAHALNLSDSGVAVR
                     VGLPPTSRSLARAKAAGVEVAPVNEVAAWADVVMVLAPDTVQAKLYENEIAPHLNAGK
                     TLMFAHGFNIRYGTITPRNDIDVSMIAPKGPGHRVRETYRDGNGVPALMAVHQDATGT
                     ARHTALSYASAIGAGRAGVLETTFAEETETDLFGEQAVLCGGVSALVKSAFETLTAAG
                     YQPEVAYFECLHELKLIVDLMYRGGLSYMRYSVSDTAEYGDYVSGPRVVDPRVKETMK
                     KVLDEIQNGAFAKRWIEENQTGRRWFEEERKRETQHQIEEVGTQLRSMMPFLDPVTIK
                     PGE"
     gene            4020..5588
                     /locus_tag="VFV99_15285"
     CDS             4020..5588
                     /locus_tag="VFV99_15285"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5060731.1"
                     /translation="MTDYVRIFDTTLRDGEQSPGATMTSAEKLEVARALARLRVDVIE
                     AGFPAASPDDLAAVRAIAEEVGSAADAPIICGLARAAHSDIRAAWEAVKGAKRPRIHT
                     FLATSDLHMQHKLRMSRAEVKARVAEMVGYAKLLCEDIEFSPEDAGRSEPAFLYEVLE
                     LAIKAGATTLNIPDTVGYTTPDEFGALIAGIRDNVPGAKDVILSVHCHDDLGLATANA
                     LAGILAGARQAEVTINGIGERAGNSSLEEVVMALHTRAAKFGLRTGIDTTQLTRVSKL
                     VTQCTGMTVQPNKAIVGANAFAHESGIPQDGMLKHEATYEIMRPETVGATQTRLVLGK
                     HSGRAALAARLGELGYTIDPTALDRVFARFKEVADRRKHITDADLEVLVRDEGQQLDE
                     EFTLDALHVGCGTLGMPTATVRLRDREGVVRAHASIGTGPVDAAYKAIDALVAAPAQL
                     LEYSVHSVTEGIDALGHVTVRVRDDRGRVCYGAGADTDIIVASVQAYLRALNRIVSGV
                     VKTSPQSWQGAEAS"
     gene            5585..>6887
                     /gene="leuC"
                     /locus_tag="VFV99_15290"
     CDS             5585..>6887
                     /gene="leuC"
                     /locus_tag="VFV99_15290"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5060732.1"
                     /translation="MKRTLFDKIWDAHVVTEEAGRPAVLYIDLHLVHEVTSPQAFAGL
                     EARGLKVRRPDRTVATMDHSIPTTKLVVVDDAARTQLRTLAENCKKHGITLHDTEAEH
                     TGIVHVIGPELGHTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLASQCLLQRK
                     PKSLEVRFEGTLRPGASAKDLVLAMIAKLGVGGATGHVIEYTGPAIRALDMEGRMTVC
                     NMSIEAGARAGMIAPDETTFAWLAGRKHAPQGAAWATAVARWCELRTDEGATYDRTLV
                     IDASQIEPMITFGTNPGMGIPITANVPSPDDVEPSQRAGLAKALRYMGLAPGEPLLGR
                     PVDVVFIGSCTNARLPDMRAAASVMRGNKVAAGVRVLVVPGSKDVKRAAEAEGLDQVF
                     RDAGAEWRDPGCSMCIAMNGDALAPGEYAVSTSNRNFEGRQG"
BASE COUNT         1209 a         2335 c         2320 g         1023 t
ORIGIN      
        1 ccgaaggggc tgtgacccgg ccccgtcaga ccacgacgaa ggagcccgca tgaaatcgct
       61 acagcccgat ccactgcaag gttcgttcac tctgcgcggc gcgaaggtcg tcgtcctcgg
      121 ttacgacgag gacgcacgcg agcacgcgct ggcgctcagg cgcgccaaca acagcgtgct
      181 catcggcgtt agacccgaca ccacatgctg gatgcgggcg tgcgccgatg gctttgcggc
      241 cgatcggccg agcgcgatcg tcggcagcgc cgaggtcgtc gtgatgctcg tgcgcgagcc
      301 gatggcaacg tgggcgaagg tcgagccgct gctcgctccc ggcgcgctcg tcgtgtttgg
      361 caacgcgcgg ctgctcgaag cgatgacgta ttcacgcggc ggattcgaca gcgtgctcgt
      421 caccaccgtc gacgacgcgc ataccggctg ccgtgtcgcc gtacaccgcg atgcgaccgg
      481 caaggcgcta cttcgtgccg tcggctacgc acgcgccgca tgcggcatcg acgttgcatt
      541 gcgtgcgacc tcggtggagg cagaggcgga tctcgagctc gcgtcgcacg gggagcgcgc
      601 gggcagcctg ctcgcgctgg ctgcgtcgtg cgagtgcctg ccggtcgcca agccgcaggc
      661 gatgatcgtc gacgacgacg aggacgagaa agagccgagc tggctcgatt cgatgctcgc
      721 gtggcggagc cgcatgtgac cactcgcacc ggtgcacaga tcctgtggga cgcgctccat
      781 cgcgagggcg tcgatgtcgt gttcggttat ccgggcggcg cgatcatgcc tgcgtacgac
      841 gcgctgctcg atagcccgat ccgccacgtg ctcgtgcgtc acgagcaagg cgcggcgcac
      901 atggccgatg gctccgcgcg cgtctcgggc aaggtcggcg tcgccgtcgc gacctccggt
      961 ccaggtgcga cgaacctcgt caccggcatc gcgactgcga tgctcgactc ggttccgctc
     1021 gtctgcatca cgggtcaggt ggcctcgcgc ttcatcggca gcgacgcgtt ccaggagacc
     1081 gacatcaccg gcgtcacgct gccgatcacg aagcacaact atctggtcac gcgcccgcag
     1141 gacatcgcga agacgatccg cgaggcgttc tatgtcgcgc gctcgggccg ccccggtccg
     1201 gtgctcgtcg acatcacgaa ggacgcgcag cagatgagct gcgaaccggt gttctccgac
     1261 gagcccgtgc gcatgcgcgg ctaccgtccc gaccatcgcg cggggctcga cgatctgaaa
     1321 cgcgccgccg agctgatcga tcgcgcgaag cgcccggtga tcctcgccgg ccacggcatc
     1381 gtcaaagccg aagcgaccac actgctccgc gcactcgtcg tcaagactgg catgccggtc
     1441 gcgtcgacgc tgctcggcct cggcggcttc ccggccacgc atccgctcag cctcggcatg
     1501 atgggcatgc acggcgaagc gtgggtcaac caggcgatcc aggaagccga cctgctcgtc
     1561 gcgctcggca tgcgcttcga cgatcgcgtc accggcaagc tcgacacgta tgcggtcaac
     1621 gcgaagaaga tccactgcga gctcgacccc gccgagatca acaagatcgt gcgcgcagac
     1681 gtcgcgctcg tcggcgacgt gcgcgaaacg ctcgaagcgc tgctgccgct cgtcgaggcg
     1741 cgcgaccacc acgactgggt ggggcgcatc agcgagctca agggtgactc cgcggttcgc
     1801 gacatccagc agatgccgcc cgccggcaag ctctacgccg cgcacgtgat gcacgacctg
     1861 tggcgcatca ccgaggggaa ggcgctcgtc gtcaccgacg tcggccagca ccagatgtgg
     1921 gaagcgcagt actacaagca cgatcatccg cgaaagctcg tcacgtcggg cggcctcggc
     1981 acgatgggct tcgcgctgcc cgctgcactc ggtgcgcgct tcgcagcgcc cgatgacgag
     2041 atctgggtga tcgccggcga cggtggcttt cagatgaccg cgtgcgagct gtcgacgatc
     2101 gcgcaagagg gcgtgaagct gaacatcgcg atcatcaaca acggctacct cggcatggtc
     2161 cgccagtggc agcagttctt ctacaacaac cgctactcgc agacgccgct caagagccct
     2221 gacttcgtga agctcgccga ggcgcacggg ctgttcggcc tgcgcgtgca atcacgcgaa
     2281 gaggtcgcgc ccgcggtgat gcgtgcgcgc gccgcacgcg gaaccgtgct gatcgatttc
     2341 cgcgtcgagc aagaagactc cgtttacccg atggtgccgg ccggcgcggc actccacgac
     2401 atgatccgcc ggcccagtcc catcgtcgaa accgcagagg acgactagcc atgaacatcc
     2461 gcctacgcac gttcatcatg cacgtcgaag atcttcccgg cgtgctcaac cgcgtgacgt
     2521 cgctattccg ccgtcgcggc tacaacatcg agtcgctcgc cgtcggccgg accgaggttg
     2581 ccggcgtgtc gcgcatgacg atcgtcatgg aagccgacga cgatgcagcg cgccgcatcg
     2641 aggcgaacct ctacaagctc gtcaacgtgc tccgcgtcga ggacacgacg tggcggccga
     2701 cggtgtcgcg cgagctcgcg ctgatcaagg tgcgcacgaa cgaggcgaac cgcgcgcagg
     2761 tgctccagct caccgaggtg tttcgcgcgc gcgtgatcga cgtcagcccc gaagccgtga
     2821 tcttcgagat caccggcacg cgcgacaagg tcgacagtct cgccgaggtg ctcgcgccct
     2881 acggcgtcgt cgagctggtg cgcactggtg ccatcgcgat ggtgcgcagc gccgaggcac
     2941 tcgaagcaac cccaaccgtt tcagaacagc cgcagacgaa cgcggcgtga aaggcaacga
     3001 ccatggcaac gatctattac gacaaggatg cggacctctc actgatccgt ggcaagcgcg
     3061 tcgccatcgt cggctacggc tcgcaaggcc acgcccacgc tctcaacctc tcggatagcg
     3121 gcgtcgccgt tcgcgtcggt cttccaccga cgagccgcag cctcgcgcgc gcgaaagcgg
     3181 ccggcgtcga agtcgcgccg gtcaatgaag tcgccgcctg ggcagacgtc gtgatggtgc
     3241 tcgcgcccga caccgtgcag gccaagctct acgagaacga gatcgcgccg cacctcaacg
     3301 ccggtaagac gctgatgttc gcgcacggct tcaatatccg gtacggcacg atcacgccgc
     3361 gcaacgacat cgacgtgtcg atgatcgcgc cgaagggtcc tggccatcgc gtccgcgaga
     3421 cgtaccgcga cggtaacggc gtgccggctc tgatggccgt gcatcaagat gcgaccggca
     3481 ctgcccgcca caccgcgctg tcctatgcga gcgcgatcgg cgctggccgc gcgggcgtgc
     3541 tcgagacgac gttcgccgag gagaccgaga ccgatctgtt cggcgagcaa gccgtgctgt
     3601 gcggcggcgt cagcgcgctc gtgaagagcg ccttcgagac gctcaccgcg gccgggtatc
     3661 agcccgaggt cgcgtacttc gagtgcctgc acgagctgaa actgatcgtc gacctcatgt
     3721 accgcggcgg cctcagctac atgcgctact cggtgagcga cacggccgag tacggcgatt
     3781 acgtctcggg gccgcgcgtc gtcgacccac gcgtgaagga gacgatgaag aaggtgctcg
     3841 acgagatcca gaacggcgcg ttcgcgaagc gctggatcga ggagaaccag acgggtcgcc
     3901 gctggttcga agaagagcgc aagcgcgaga cgcagcacca gatcgaagag gtcggtacgc
     3961 agctgcgctc gatgatgccg ttcctggatc ccgtcacgat caaaccgggc gagtaagcca
     4021 tgaccgacta cgtacgcatc ttcgacacca cgcttcgcga cggcgagcaa tcgcccggtg
     4081 ccacgatgac ctcggccgag aagctcgagg tcgcgcgtgc actcgcgcgg ctacgcgtcg
     4141 atgtcatcga agcggggttt ccggccgcat cgccggatga cctcgccgcc gtacgagcga
     4201 tcgccgagga agttggcagt gcggcggatg ctccgatcat ctgcgggctt gcgcgcgcgg
     4261 cgcacagcga catccgtgcc gcgtgggagg ccgtgaaggg cgcgaagcgt ccgcgcatcc
     4321 atacattcct cgcgacgagc gacctccaca tgcagcacaa gctgcggatg tcacgcgcgg
     4381 aggtgaaggc acgcgtcgcc gagatggtcg gctacgcgaa gctcctgtgc gaggacatcg
     4441 agttctcgcc ggaggatgca ggccgcagtg agcccgcatt cctctacgag gtgctcgagc
     4501 tcgcgatcaa agccggcgcg accacgctca acatccccga caccgtcggc tacacgacgc
     4561 ccgacgagtt cggcgcactg atcgcgggta tccgcgacaa cgtgcccggt gcgaaggacg
     4621 tgatcctctc ggtgcactgc cacgacgacc tcgggctcgc gactgcgaat gcgctcgctg
     4681 gcatcctcgc gggtgcccgg caggcggagg tcacgatcaa cggtatcggc gaacgcgccg
     4741 gtaactcatc gctcgaagaa gtcgtgatgg cgctccacac gcgcgccgcg aagttcggcc
     4801 tgcgcaccgg tatcgatacg acgcagctca cgcgcgtgag caagctcgtc acgcagtgca
     4861 cgggcatgac ggtgcagccg aacaaggcca tcgtcggcgc gaacgcgttc gcgcacgagt
     4921 cgggcatccc ccaggacggc atgctcaagc acgaggcgac gtacgagatc atgcggccgg
     4981 agacggtcgg cgcaacgcag acgcggctcg tcctcggcaa gcactcggga cgcgcggcgc
     5041 tcgcggcgcg gctcggcgag cttggctata cgatcgatcc caccgcgctc gaccgcgtgt
     5101 tcgcgcgatt caaggaggtc gccgatcggc gcaagcacat caccgacgcc gacctcgaag
     5161 tgctcgtgcg cgatgaaggg cagcagctcg acgaggagtt cacactcgac gcgctgcacg
     5221 tcggctgcgg cacgctcggc atgccgacgg cgacggtgcg cctgcgcgat cgcgaaggtg
     5281 tcgttcgcgc gcacgcgtcg atcggcactg gaccggtcga tgctgcctac aaggcgatcg
     5341 atgcgctcgt tgcggcgccg gcacagctgc tcgagtactc ggtgcactcg gtgaccgaag
     5401 gcatcgacgc gctcggccac gtgaccgtgc gcgtgcgcga cgaccgcggc cgcgtgtgtt
     5461 acggcgcggg cgccgacacc gacatcatcg tcgcaagcgt gcaggcctac ctgcgcgcgc
     5521 tcaatcgcat cgtgagtggc gtcgtgaaga cgtcgccgca gagctggcaa ggggcagaag
     5581 catcatgaag cggacgttgt tcgacaagat ctgggacgcg cacgtcgtga ccgaagaggc
     5641 agggcgaccg gcggttctct acatcgactt gcatctcgtg cacgaggtga cgtcgccgca
     5701 ggcatttgcg ggactcgaag cgcgcggcct gaaggtccgc cggcccgatc gtacggtcgc
     5761 gacgatggac cactcgattc cgacgacgaa gctcgtcgtc gtcgacgatg cggcgcgcac
     5821 gcagctgcgc acgctcgccg agaactgcaa gaagcacggc atcacgctgc acgacaccga
     5881 ggccgagcac acgggcatcg tccacgtgat cggtcccgag ctcggtcaca cccagccggg
     5941 catgacgatc gtgtgcggcg atagccacac gtcgacgcac ggcgcgttcg gcgcgctcgc
     6001 gttcggtatc ggcacgagcg aggtcgagca cgtgctcgcg tcccagtgcc tgctccagcg
     6061 caagccgaag tcactcgaag tgcggttcga gggcacgttg cgcccgggcg cgtcggccaa
     6121 ggacctcgtg ctcgcgatga tcgcgaaact cggcgtcggc ggcgcgaccg gtcacgtgat
     6181 cgagtacacg gggcccgcaa tccgcgcgct cgacatggaa ggccgcatga cggtctgcaa
     6241 catgtcgatc gaggcaggcg cgcgtgccgg catgatcgcg cccgacgaga cgacgttcgc
     6301 gtggctcgcc ggccgcaagc acgcgccgca gggtgccgcg tgggccacgg cggtcgcgcg
     6361 ctggtgcgag ctgcgcaccg acgagggcgc gacctacgac cgcacgctcg tgatcgacgc
     6421 gtcgcagatc gagccgatga tcacgttcgg cacgaatccc ggcatgggca ttccgatcac
     6481 ggcgaacgtg ccgtcacccg atgacgtcga gccgtcgcag cgcgcgggcc tcgcgaaggc
     6541 gctgcgctac atgggcctcg cgcccggcga gccgctgctc ggccggccgg tcgatgtcgt
     6601 gttcatcggt tcgtgcacga atgcgcggct tcccgatatg cgcgccgccg cgagcgtgat
     6661 gcgcggtaac aaggtcgcgg ccggcgtgcg cgtgctcgtc gtccccggca gcaaggacgt
     6721 caagcgcgcc gcggaagccg aaggcctcga ccaggtgttc cgcgacgccg gcgctgaatg
     6781 gcgcgacccg ggctgctcga tgtgcatcgc gatgaacggc gatgcgctcg cacccggcga
     6841 gtacgcggtc tcgacctcga accgcaactt cgaaggtcgt cagggcc
//
LOCUS       DASUFX010000382        13397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1590603, whole genome shotgun sequence.
ACCESSION   DASUFX010000382 DASUFX010000000
VERSION     DASUFX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590603"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFV99_15295"
     CDS             <1..918
                     /locus_tag="VFV99_15295"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060733.1"
                     /translation="KPANVLHAGDNRWKLADFGIARMPDSDLTQVGIFMGTPGYSPPE
                     AIKEGRYTPQADVFAWGAVMYELMSGRIPYEGPDTKTTNGYVVQGNAPPPTRHDASIP
                     EPLSTVTMTALQPSDKARFKDASEAEQALREAWERCLTQGLVHPAVLAMDEMPHDKAA
                     VRFHEPRGAVARPAPVVLGDSSEMTAVNDKPGPKKSNQFVIESSKGRPGDDDPTKLMV
                     REENNKAKPDATARGVGPTGTQTPSAEVKSGARAALDSAADTKADPAISRAMKGEGGG
                     ARRAILIAVIALLVLGSAGAGLYFGGMFG"
     gene            1077..1328
                     /locus_tag="VFV99_15300"
     CDS             1077..1328
                     /locus_tag="VFV99_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060734.1"
                     /translation="MSSLADRDAIMLIGDAGLERVLRVISASRVFVARQPLDAIRILV
                     EHAESLQMVVVAAELEWRAELAELLEAEYPEVRLVVVSP"
     gene            complement(1325..2275)
                     /locus_tag="VFV99_15305"
     CDS             complement(1325..2275)
                     /locus_tag="VFV99_15305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5060735.1"
                     /translation="MEVTVVGAGVIGLTTALTLEERGHRVRIVAAARRADTTSAIAGA
                     VWFPYRVGPPNKVTVWAMRTRKWLEQLAGTPEAGIDVLTGYEILGDDGTTRPWWAAAP
                     TMEGDGLIESIDVEWVPSPVVGAPAGWKFTAPRAQPSLFLPFLESLIEGEIEQRIVTN
                     IHDEPGDVVINCTGLGARDLVGDDKLYGLLGQIVIAERGSADLATTVTDDRDPERIFY
                     IIPRRDELVLGGCSLPFPPGDTPQLDPDITARILAHAKRLGISVGAVRTERVGLRPYR
                     AEVRLERDPHHKRVIHNYGHGGAGFTLCRGCAEDVATLLG"
     gene            2321..3076
                     /locus_tag="VFV99_15310"
     CDS             2321..3076
                     /locus_tag="VFV99_15310"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX5060736.1"
                     /translation="MTRSVDVLTIVLLSIAALGAGTVDAIAGGGGLITVPALLTAGLP
                     PVQALGTNKGQSVWGSGAALIAFWRADRVDRKQASYTFGLGFLGSLGGATLVNAIDKD
                     ALKPIVIAMLIGAAVLLVIRKPSRDQDAGHAKWYVAALLALVIGAYDGFFGPGTGTFL
                     IVGFVTLCGRSLVHASADAKVVNFASNLAAICIFTIHDAIVWRVAIPMAFGQLLGGVI
                     GAQLAMKGGERVVRGVVLCVSGALILKLVYDLI"
     gene            3089..3571
                     /locus_tag="VFV99_15315"
     CDS             3089..3571
                     /locus_tag="VFV99_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060737.1"
                     /translation="MRTVCLVVLLLAACKDKQEAKPTPAPTPALAQVVPEKTEAPPPE
                     APRPAAPAAVKPAGGLNTQAEYEAKAFELTDKLAEVFAAAGTNCPKLADNIERFAAAN
                     QQAFAGTEEFEQANPTAEDELEPKLREKSTGLMQKISLSMQACQKDERVKAALAKLPK
                     "
     gene            complement(3578..4513)
                     /gene="lipA"
                     /locus_tag="VFV99_15320"
     CDS             complement(3578..4513)
                     /gene="lipA"
                     /locus_tag="VFV99_15320"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5060738.1"
                     /translation="MSERLHLPVVQGGEAAPKRRHPEWIRAQLPSKPEYFEVRNMVRE
                     LKLHTVCESASCPNIGECWTRRALTIMILGDICTRSCRFCDVTTGRPLPADPDEPRRV
                     AEMLGRLQLEHTVITCVDRDDLADFGAAHWAETIRRVKEACPQMTLEVLAGDFKGKTE
                     LVDIVLEADPDVFAHNLETVPRLSRQVRVQASYPRSYKILEHARKRGAVTKTGLMLGL
                     GETIEEVHEVIRELASLGVDILTLGQYLSPSKEHLAIERYVHPTEFAELEQFALAAGL
                     THVVSGPLVRSSYHADGQAKLVRDLRAAKGLAPIS"
     gene            complement(4555..5892)
                     /locus_tag="VFV99_15325"
     CDS             complement(4555..5892)
                     /locus_tag="VFV99_15325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5060739.1"
                     /translation="MFKAVVISDAVASPDLIVASVDLVSRALLDAGCTDVERVSSIEH
                     GLSLVNRLQAAVIILDLLEPLRFEGYRRILARSRGVPVIVIVPENKMQTAFDAGVDDC
                     VARPIRRVELVARTRAAIRVRIERARRSKRDRRLSQEVRTLQREKHDLERIVCVDSLT
                     GVANRRHSLSLLEAEWKRSAREGTPMSLIIIDLDCFHAFNETYGHPGGDACLRRVTAE
                     MVMCLRRPSDFLGRYGGEEFIAVLANTDAAGACIVAERLRAAVEAIQLPHISSTCAHV
                     VTISVGFATATPQPDATHDALVSAADDALLAAKSHGRNRINGEAAAHGAVRHRMWTQP
                     FRRFPPVVADPWFANRIPQFLAAARGELLAARDACESGGFDRIRATARKLRGNANDHG
                     IETIAELANLLERAARTDDRASIKAVVDELEQYVEHVQVTYRRPLERKLGHVG"
     gene            complement(6290..7744)
                     /gene="lpdA"
                     /locus_tag="VFV99_15330"
     CDS             complement(6290..7744)
                     /gene="lpdA"
                     /locus_tag="VFV99_15330"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5060740.1"
                     /translation="MSDPKSNGSAGAASAAAGPYDVVVIGSGPGGYVAAIRAAQLGLK
                     TACIERAELGGICLNWGCIPTKALLKSAEVLEYAQRAKEFGVVIEGVRADFPAIIKRS
                     RDVADKISKGVGFLFRKNKIDSIAGTAKLLPRSGPWKPHQVEVTSDAGKRIIDTKNVI
                     IATGARARSLPGIELDGKRIIEYRKAMTLPEQPKTMVVLGAGAIGVEFASFYRALGVD
                     VTIVEYLPRLVPNEDPEVSKELGKAFDKKGIKLLVSHKLSNAKNTGNAVTMTVEPAAG
                     GDKKEITADILLVAVGIAANTEGIGLEQHGIQLEKGFIKVDAECRCGDGLWAIGDVAG
                     RGLAHVASAEGVFVAETIAGVHAEPVRYDAIPACTYCHPEIASVGLTEEKAKAQNIPV
                     KVGRFPFRPLGRAMASGEATGFIKVMWHGETGALVGAHMIGPAVTDLIAELTLAKTTE
                     VNAESLIYTIHAHPTFAEAIRGASEDAFGHAIDL"
     gene            complement(7741..9150)
                     /locus_tag="VFV99_15335"
     CDS             complement(7741..9150)
                     /locus_tag="VFV99_15335"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX5060741.1"
                     /translation="MAQIIGLPKLSPTMEEGVLVRWTKKEGDKVSPGDLVAEVETDKA
                     NMDFNIEDDGVLLKLLVKEGETVKLGAPVAIIGKAGEDVTALVAEAAKQGGGAPAAKQ
                     EAPKQEAPAKQQPPQQAPAARGPEVSATAPAAKKPTPAQAAPAPKAAAPAPAAGGKLL
                     ASPLAKTLAIELGIDLRAIQGSGPGGRIVERDVRAAASGGGNGHAQAPAHDGGGAEAE
                     VDETQTTQALAVRHARQMTPDTEGDYTDIPASNMRKRIAARLTEAKRDVPHFYLMRTI
                     DAAPMLGFRGRLNELLGDKGKVSVNDLVIKAVALALRRVPECNAMWEGDAIRQFHRVH
                     IGVAVALDEGLITPVVRDADQKGIGSIAAEVRDLADRAKHKRLKAHEISGSTFSVSNL
                     GMMGIERFTAIINPPEAGILAIGTVVETPVVKDGQIVVGKRMTVTMSCDHRVIDGALG
                     AKWLAAFCDLIEKPESLAL"
     gene            complement(9154..10146)
                     /locus_tag="VFV99_15340"
     CDS             complement(9154..10146)
                     /locus_tag="VFV99_15340"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX5060742.1"
                     /translation="MSVISYREALNQAMREEMERDKDVFIMGEEVGYYNGAYKVTQGL
                     LQRFSERRVVDAPIAEMGFAGIGVGAAMVGLRPIIEFMTFNFSLVAIDQIVNNAAKIY
                     QMSGGQYNVPIVFRGPGGPAVQVGSQHSQSLESYYAHVPGLKVVMPSTAYDAKGLLKT
                     AIRDDNPVVFIEGETLYNTTWEVPDAAEGSEHLIPLGLADVKREGKDVTLIAWSKMVW
                     TCLDAAKVLAEEGIECEVVDPRTLRPLDVETLAESVRKTGRCVIVEVGWPVSGFGAEV
                     SYQMQKHCLDSLDAPIERVTSDDVPMPYAKNLEDEVQPQVKDVVAAVHRVLYLD"
     gene            complement(10150..11163)
                     /gene="pdhA"
                     /locus_tag="VFV99_15345"
     CDS             complement(10150..11163)
                     /gene="pdhA"
                     /locus_tag="VFV99_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5060743.1"
                     /translation="MATASMSTPETKRIEPEESAVETTVEELRPLYRQMLAIRRMEEA
                     SAKAYSQGKIGGFLHLIIGQEAVCVGAVAALEPKDYVVATYREHGHAYAKGVSARAVL
                     AELYGKKTGCVKGLGGSMHIFDKSKNFLGGYGIVGGHVPIAAGAAFASKYRGDQAVTL
                     CFFGEGASTIGGFAEGLALAGLWKLPVVLICENNQYSMGTPLYRSLAVEDVSMRALAH
                     GMARDRFDGDDVVKVKRRIAEAVERARQSSEPTLVEVVTYRFRGHSMSDPGLYRTKDE
                     VEEWKRRDPLNRSRARLTELGMTEDELTSLEADVKAEIEDAVKFAEESPPADEYLPYV
                     VKE"
     gene            complement(11202..11603)
                     /locus_tag="VFV99_15350"
     CDS             complement(11202..11603)
                     /locus_tag="VFV99_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060744.1"
                     /translation="MLRFATLVLVLAGCPGGGDECHVDSDCGHNLVCARNSDCLPASE
                     VRSIRVTWTIRGMPANEMLCAKTPNFYLMFAGTSVNDTFGYEPVPCKAGLFSIDKLPL
                     RFVSVELGVDGGFSEVTAFDAQGNASFDLAP"
     gene            complement(11603..12136)
                     /locus_tag="VFV99_15355"
     CDS             complement(11603..12136)
                     /locus_tag="VFV99_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060745.1"
                     /translation="MRPRVTSRKGAARGPRPFALAALLLAGCIIEPPDPGPDPGGGSG
                     DGWGSGWGGSGGSTGYGCHSDTACGSGYVCARNGECTIASGVRIIHANWTVKQQVASD
                     ATCVSSKSLDITFSTSADSFDMFGFAPVPCDAGRYTIDKMPARYTYVTLARTGDTSGG
                     DSATFDAQGNAVLDLPY"
     gene            complement(12152..>13397)
                     /locus_tag="VFV99_15360"
     CDS             complement(12152..>13397)
                     /locus_tag="VFV99_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060746.1"
                     /translation="PARGIVHLHSPYSHDACDGMPRDATGAPNEPCLEHLRAALCTEQ
                     IDFAALTDHDDTMADEEFTTLFSMRGTDTPVMSGSNQIASRIHCDDGHEVLITIGGEN
                     DLMPIMLDHHVAGTVQERHDIYNGTSATAIQAMRDAGALIWVAHTEQHPTDELRTVAP
                     NGVELYNLHANIDPKIRADFLGLDGYGAITNAVEFADTNEGGPEPDLALLSFLTPNLP
                     GITHWNELLAEGKHIPATAGSDAHENALPVILADGERGDSYRRVLRWFSNIVLVNDPK
                     DITQIEAALAKGRLFSVFEIMGTPDGFDVVATGGGELGDTVTFNATHLLVKVPTVRGL
                     DPTLPAPEIKATVIRCSAGAVTTVASGSGPELDVELPGPGAYRVEVSIVPRHLGPYLR
                     DLGTAWADQELPWIYTSAIYVE"
BASE COUNT         2130 a         4402 c         4469 g         2396 t
ORIGIN      
        1 aagccggcga acgtgctgca cgccggtgac aaccgctgga agctggccga tttcggcatc
       61 gcgcgcatgc ccgacagcga tctcacgcag gtcggcatct tcatgggcac gcccggctac
      121 tcgccgcctg aagcgatcaa agaaggccgc tacacgccgc aggccgacgt attcgcgtgg
      181 ggcgcggtga tgtacgagct gatgtcggga cgcattccgt acgagggccc cgacacgaag
      241 acgacgaacg ggtacgtcgt gcagggcaac gcgccgccgc cgacgcgcca cgacgcatcg
      301 attcccgagc cgctgtcgac ggtgacgatg accgcgctgc agccgagcga caaggcgcgc
      361 ttcaaggacg cctcggaggc ggagcaagca ctgcgcgaag cgtgggagcg ctgcctgacg
      421 caaggcctcg tgcatcccgc ggtgctcgcg atggacgaga tgccgcacga caaggcggcg
      481 gttcggttcc acgagccgcg tggcgcggtc gcgcgcccgg cgccggtcgt gctcggtgat
      541 tcgagcgaga tgaccgcggt caacgacaag ccgggaccga agaagtcgaa ccagttcgtg
      601 atcgagagct cgaagggtcg tccaggcgat gacgatccga cgaagctgat ggtgcgtgag
      661 gagaacaaca aggcgaagcc cgatgcgacg gcgcgtggcg tcggtcccac cgggacgcag
      721 acgccgagtg ccgaagtgaa gtccggtgcg cgcgcggcgc tcgacagcgc cgctgatacg
      781 aaagcggatc ctgcaatctc gcgcgcgatg aagggcgagg gtggcggggc ccgccgcgcg
      841 atcttgatcg cagtgatcgc gttgctcgtc ctgggcagcg ctggcgcggg cctctacttc
      901 ggcggcatgt tcggctgatc gtctaggcga cagtcgtcgt tcagcgtccc gacacccacg
      961 accgccgagc tcgtgatttc gcacatgtcg cgccgagcgg cgccggccca cgatttgctc
     1021 gtgtacgtcg cgtggtgctg aaaggatcgc tggcagtcgc tgtcggcgac gcaggcatga
     1081 gctcgctcgc tgaccgggac gcgattatgt tgatcggcga tgccggcctc gagcgcgtgc
     1141 tgcgcgtgat ttctgcgtcg cgcgtattcg tagcgcggca gccgctcgac gcgatccgga
     1201 tcctcgtcga gcatgccgag agtctccaga tggtcgtcgt cgccgcagag cttgaatggc
     1261 gtgcggagct ggccgagcta ctcgaggccg agtatcccga ggttcgcttg gtcgtcgtgt
     1321 cgccttagcc gagcagcgtc gcgacatcct ccgcgcagcc gcggcacagc gtgaacccag
     1381 cgccgccgtg gccgtagttg tggatgacgc gcttgtggtg cggatcgcgc tcgaggcgaa
     1441 cctcggcgcg gtacggccgt agaccgacgc gctcggtgcg caccgcgccg accgagatgc
     1501 cgagccgttt cgcgtgcgcg aggatgcgcg cggtgatgtc cggatcgagc tgtggtgtgt
     1561 cgcccggcgg gaacggcagc gagcagccgc cgagtacgag ctcgtcgcgg cgcgggatga
     1621 tgtagaagat gcgctcggga tcgcgatcgt cggtgacggt cgtcgcgagg tcggcgctcc
     1681 cacgctcggc gatgacgatc tggccgagca ggccgtacag cttgtcgtcg ccgacgagat
     1741 cgcgggcacc gaggccggtg cagttgatga cgacgtcgcc gggctcgtcg tgaatgttcg
     1801 tgacgatgcg ctgctcgatc tcgccttcga tgagcgactc gagaaacggc aggaacagag
     1861 acggctgcgc gcggggggcg gtgaacttcc agccagccgg tgcgccgacg acaggcgagg
     1921 gcacccactc gacatcgatg ctctcgatga ggccgtcgcc ctccatcgtc ggtgcggctg
     1981 cccaccacgg gcgcgtcgtg ccgtcgtccc cgaggatctc gtagccggtc agcacgtcga
     2041 tgccggcctc cggcgtgccg gcgagctgct cgagccattt gcgcgtgcgc atcgcccaga
     2101 cggtgacctt gttcggcggc ccgacgcgat acgggaacca gacggcaccc gcgatcgcgg
     2161 aggtagtgtc ggcgcggcgg gcggccgcga cgatgcgcac gcggtggccg cgttcttcga
     2221 gcgtgagtgc cgtggtcagg ccgatgacgc ctgcgccaac gacggttact tccatccgac
     2281 aagcatagcg aggactggtg gccgcgacga attacgcgct atgacgagat ctgtggatgt
     2341 cctgacgatc gtgctgttgt cgatcgccgc gcttggcgcg ggtacggtcg atgcgatcgc
     2401 gggagggggc ggcctgatca cagttccggc gctgctcact gccggattgc cgcccgtaca
     2461 ggcgctcggc acgaacaagg gtcagagcgt atggggctcg ggtgcggcgc tgatcgcgtt
     2521 ctggcgtgcg gaccgcgtcg atcgcaagca ggcctcctac acgttcgggc ttggcttcct
     2581 cggctcgctc ggtggcgcga cgctcgtcaa cgcgatcgac aaggacgcgc tcaagccgat
     2641 cgtgatcgcg atgctgatcg gcgcagcggt gctgctggtc attcgcaagc cgtcgcgcga
     2701 ccaggacgcg ggccacgcca agtggtacgt ggcggcgctg ctcgcgctcg tgatcggcgc
     2761 ctacgacggc ttcttcgggc ccggcaccgg gacgttcttg atcgtcggct tcgtgacgct
     2821 gtgcggccgc agcctcgtcc acgcgagcgc ggacgcgaag gtcgtcaact tcgcgtcgaa
     2881 cctcgccgcg atttgcatct tcacgattca cgacgcgatc gtctggcgcg tcgcgattcc
     2941 gatggcgttc ggccagctcc tcggcggcgt gatcggcgcc cagctcgcga tgaagggcgg
     3001 cgaacgcgtc gtccgcggcg ttgtactttg cgtgtcaggt gcgctgatcc tcaagctcgt
     3061 gtacgacctg atctagtatc gtcggcgcat gcgcaccgtc tgcctcgtcg tgctcctgct
     3121 cgccgcctgc aaagacaagc aggaagcgaa gccgactcct gcaccgaccc cggcgctggc
     3181 gcaggtcgtg ccggagaaga cggaagcgcc gccacccgaa gccccaagac cggcagcgcc
     3241 cgccgcggtc aaaccggccg gcggtttgaa cacgcaggcg gagtacgagg cgaaggcctt
     3301 cgaactcacc gacaagctcg ccgaggtgtt tgcggcggcg ggcacgaact gcccgaagct
     3361 cgccgacaac atcgagcggt tcgcagccgc caaccagcag gcattcgccg gcaccgaaga
     3421 gttcgaacag gcgaatccga cggccgagga cgagctcgag cccaagcttc gcgagaagtc
     3481 gacgggcctc atgcagaaga tcagcctctc gatgcaggcg tgccagaagg acgagcgtgt
     3541 gaaggcagcg ctcgcgaagc tgcccaagta gttgcgctca gctgatcggc gccaggccct
     3601 tggccgcgcg cagatcgcgg acgagcttcg cttgaccgtc ggcgtgatag ctcgagcgca
     3661 cgagcggacc gctcacgaca tgcgtgaggc cggcggcgag tgcgaactgc tcgagctcgg
     3721 cgaactccgt cggatgcacg tagcgctcga tcgccaggtg ctccttgctc ggcgacaggt
     3781 actggccgag cgtgaggatg tcgacgccaa gcgatgcgag ctcgcggatc acctcgtgta
     3841 cctcttcgat cgtttcgccg aggccgagca tcaggccggt tttggtgacc gcaccacgct
     3901 tgcgcgcgtg ctcgaggatc ttgtagctgc gcggatagct cgcctgcacg cggacctggc
     3961 gcgacaggcg cggcacggtc tcgaggttgt gtgcgaacac gtcgggatcc gcttcgagca
     4021 cgatgtcgac gagctcggtc ttgcccttga agtcgccggc gagtacctcg agcgtcatct
     4081 gcgggcacgc ctctttgacg cggcggatcg tctctgccca gtgcgcggcg ccgaagtcgg
     4141 cgaggtcatc gcgatcgaca cacgtgatca cggtgtgctc gagctggagg cggccgagca
     4201 tctcggcgac gcggcgtggc tcgtctggat cggccggcag cgggcggccg gtggtgacat
     4261 cgcagaagcg gcacgaccgg gtgcagatgt cgccgaggat catgatcgtg agcgcgcgcc
     4321 gggtccagca ctcgccgatg ttcgggcaac tcgcgctctc gcacacggtg tggagcttca
     4381 gctcgcgcac catgttgcga acctcgaagt actcgggctt gctcggcagc tgcgcgcgaa
     4441 tccactcggg gtggcggcgc ttgggcgcgg cctcaccgcc ctgaaccacg ggaaggtgaa
     4501 ggcgctcgct catgggcacc atgtagcacg cgcggccggg atccagaagt ggtgtcagcc
     4561 cacgtggccg agcttgcgtt cgagcggcct gcgataggtg acttgcacgt gctcgacgta
     4621 ctgctcgagc tcgtcgacaa ccgctttgat cgacgcgcga tcatcggtgc gtgccgcgcg
     4681 ctcgagcagg ttcgcgagct cggcgatcgt ctcgatgcca tgatcgttcg cgttgccgcg
     4741 gagcttgcgc gccgtcgcgc gaatgcgatc gaaaccgccg gactcgcagg catcgcgggc
     4801 ggcgagcaat tcgccgcgtg ccgctgcgag aaactgcggg atgcggttcg cgaaccatgg
     4861 atcggcgacg accggtggga accggcgaaa tggctgggtc cacatccggt ggcgcactgc
     4921 gccgtgggcg gccgcttcgc cgttgatccg gttgcggccg tgcgacttcg cggcgagcag
     4981 cgcatcatcg gctgcgctga ccagcgcatc gtgggtcgcg tctggctgcg gcgtcgcggt
     5041 ggcgaagcca acgctgatcg ttacgacgtg ggcgcacgtc gagctgatgt ggggcagctg
     5101 gatcgcctcg accgcggcac gcaggcgctc cgcgacgatg caggcgcccg cggcgtcggt
     5161 attggcgagc acggcgataa actcttcgcc gccgtagcga ccgaggaagt ccgacggccg
     5221 gcgcagacac atcaccatct cggcggtcac gcgccgcaga cacgcatcac caccgggatg
     5281 gccgtacgtc tcgttgaacg cgtgaaagca gtcgaggtcg atgatgatga gcgacatcgg
     5341 cgtgccctcg cgcgcggagc gtttccactc cgcttcgagc aggctcaggc tatggcggcg
     5401 attcgcgacg ccagtcaggg agtcgacaca cacgatgcgc tcgagatcgt gcttctcgcg
     5461 ttgcaacgtt ctcacttctt gcgacagccg gcggtcacgc ttgctgcggc gtgcgcgctc
     5521 gatgcgcact cggatcgcgg cgcgcgtgcg cgcgacgagc tcgacgcggc ggatcggccg
     5581 cgcgacgcag tcatcgacgc cagcgtcgaa tgcggtttgc atcttgttct cgggaacgat
     5641 gacgatcacc ggcacgccgc gactgcgcgc gaggatccgg cgatagcctt cgaaccgcag
     5701 cggctcgagc agatcgagga tgatcacggc ggcctgcagg cgattgacga gcgacaggcc
     5761 gtgctcgatc gaggacacgc gctcgacgtc cgtgcagccc gcatccagta gcgctctact
     5821 tacgaggtcg acggacgcga caatgaggtc cggggacgca acggcgtcgg agatgacgac
     5881 ggctttgaac acccgctact ccggtccctg ttctccgccg agcacgtcgt acgggtcgtc
     5941 gggttgatgc tggtgcgcgc ggtcttcgca atcgctgcag aggaggctct cacgtgtcgt
     6001 gatctcgccg catgcttcac atgcatgagg cacgagcttc atgtgattcg cgaccaactc
     6061 gtcatcctgc tcacgagtag gcaagatgac gaacggccgt gtcgcgcgag tcgcctgcat
     6121 gcgcgcgtca tgtttcaagg catgtgccac gcgcctcgaa ttacctgggc ttacaaccga
     6181 ctacgtctct gagtttcgcc agttgcgatg actcgcgtct gcggctcata catcgtcgta
     6241 gtcgcctctg cgcgcggtgt gcgcgtccgc gcgcccaacc ggtgtgcaac taaagatcga
     6301 tcgcgtgacc gaacgcatct tccgatgcgc cgcgaatcgc ttcggcgaac gtcgggtgtg
     6361 cgtggatggt gtagatgagc gactctgcgt tgacctcggt ggtcttcgcg agcgtgagct
     6421 ctgcgatcag atcggtgacg gccggtccga tcatatgagc cccaactaat gcaccggtct
     6481 cgccgtgcca catcaccttg atgaaaccgg tcgcttcacc agatgccatc gcgcggccga
     6541 gcgggcggaa cggaaaccgg ccgaccttaa ccgggatgtt ctgcgccttc gctttttctt
     6601 ccgtgagacc gacggaagcg atttccggat ggcagtacgt gcatgcggga atcgcgtcgt
     6661 agcgcacggg ctcggcgtgg acgcccgcga tcgtttcggc gacgaacacg ccttcggccg
     6721 acgcgacgtg cgcgagtccg cggccggcaa cgtcgccgat cgcccacagg ccgtcgccgc
     6781 atctgcactc ggcatcgacc ttgatgaagc ccttctcgag ctggatgccg tgctgctcga
     6841 ggccgatgcc ctcggtgttc gccgcgatgc cgaccgcgac gagcaggatg tcggcagtga
     6901 tctctttctt gtcgccaccg gcggcgggct cgactgtcat cgtcaccgcg ttgccggtgt
     6961 tcttcgcgtt cgacagcttg tggctgacga gcagcttgat gcccttctta tcgaaggcct
     7021 taccgagctc cttactgacc tcgggatcct cgttcgggac caggcggggc agatactcga
     7081 cgatcgtgac gtcgacgccg agcgcgcgat agaacgaggc gaactcgacg ccgatcgcgc
     7141 cggcaccgag tacgaccatc gtcttcggct gctcgggcag tgtcatcgcc ttgcgatact
     7201 cgatgatgcg cttgccatcg agctcgatgc cggggagcga gcgcgcgcgg gcaccggtcg
     7261 cgatgatcac gttcttggtg tcgatgatgc gcttgcccgc atcgctcgtg acctcgacct
     7321 ggtgcggctt ccacggtccg gaacgcggca gcagcttcgc ggtgcccgcg atgctgtcga
     7381 tcttgttctt gcggaacaga aagccgacgc ccttgctgat cttgtcggcg acgtcgcggc
     7441 tgcgcttgat gatcgcgggg aagtcggcac gcacgccttc gatgacgacg ccgaactcct
     7501 tcgcgcgctg cgcgtactcg agcacttcgg cactcttcaa cagcgccttc gtcggaatgc
     7561 agccccagtt gaggcagatg ccaccgagct cggcgcgctc gatgcacgcg gtcttgagcc
     7621 cgagctgtgc agcgcggatc gcggcgacat agccacccgg acccgagccg atcacgacga
     7681 cgtcgtacgg gcccgccgcg gcgctcgcgg caccagcgga gccgttgctc ttcggatcgc
     7741 tcatagtgcc agcgattccg gcttctcgat caggtcgcag aatgcggcga gccacttcgc
     7801 gccgagtgcg ccatcgatca cccggtggtc acacgacatc gtgaccgtca tgcgcttacc
     7861 gacgacgatc tgcccgtcct tcacgacggg cgtctcgacg accgtaccga tcgcgaggat
     7921 gccggcctcg ggcggattga tgatcgccgt gaaccgctcg atgcccatca tgccgaggtt
     7981 cgacaccgag aacgtcgagc cgctgatctc gtgcgccttg agccgcttgt gcttcgcgcg
     8041 atcggcgagg tcgcgaactt ccgccgcgat cgagccgatg cctttttgat ccgcgtcgcg
     8101 cacgaccggc gtgatgaggc cttcatcgag cgcgacggcg acaccgatgt gcacgcgatg
     8161 gaactggcga atcgcatcgc cttcccacat cgcgttgcat tcgggcacgc ggcgcagcgc
     8221 gagcgcgacc gccttgatca cgaggtcgtt gacgctgacc ttgcccttgt cgccgagcag
     8281 ctcgttcaag cggccgcgga atccgagcat cggcgccgcg tcgatcgtgc gcatcagata
     8341 gaagtgcggc acgtcgcgct tggcttcagt gagccgcgcc gcgatccgtt tgcgcatgtt
     8401 cgacgccgga atgtccgtgt aatcgccctc ggtgtcgggc gtcatctggc gggcatggcg
     8461 taccgcgagc gcttgcgtcg tttgcgtctc gtcgacctcg gcctctgcac caccgccgtc
     8521 gtgcgccggt gcctgcgcgt ggccgttgcc gccgcccgac gccgccgcgc gtacgtcgcg
     8581 ctcgacgatg cggccacccg ggccgctacc ttgaatcgcg cgcagatcga tgccgagctc
     8641 gatcgcgagc gtcttcgcga gtggcgatgc gagcagcttt ccgccggcag caggggcggg
     8701 cgcagcggcc ttgggagccg gcgcagcctg cgcgggtgtg ggcttcttcg ccgctggtgc
     8761 ggtcgcgctc acttcggggc cgcgcgcggc cggtgcttgc tgcggcggtt gctgcttcgc
     8821 cggcgcttcc tgcttcggcg cttcctgctt cgcggccggt gcgccaccgc cttgcttcgc
     8881 tgcttcggcg acgagcgcgg tgacgtcctc accggccttg ccgatgatcg cgaccggcgc
     8941 gccgagcttc acggtctcgc cctctttgac gaggagcttc aacaagacgc cgtcgtcctc
     9001 gatgttgaag tccatgttcg ccttgtcggt ctcgacttca gcgacgaggt ctccgggcga
     9061 cactttgtcg ccttccttct tcgtccagcg aacgaggacg ccttcttcca tcgtcgggga
     9121 cagcttcggc agtccaatga tctgtgccat ggctcaatcc aggtagagaa cgcgatgcac
     9181 ggcagcaacc acgtctttca cttgcggttg cacttcgtct tcgaggttct tcgcgtacgg
     9241 catcggtacg tcgtcgctcg tcacgcgctc gatcggtgcg tcgagcgagt ccaggcagtg
     9301 cttctgcatc tggtagctga cctcggcgcc aaaacccgag acgggccagc cgacttcgac
     9361 gatgacgcac cgaccggtct tgcgcacgct ctcggcgagc gtctcgacat cgagcgggcg
     9421 cagcgtgcgc ggatcgacga cctcgcattc gatgccttct tcggcgagca ccttggcagc
     9481 gtcgaggcac gtccacacca tcttcgacca cgcgatcagc gtgacgtcct tgccctcgcg
     9541 cttcacgtcg gcaaggccga gcggaatcaa gtgctcgctg ccctcggcgg cgtccggcac
     9601 ttcccatgtc gtgttgtaga gcgtctcgcc ctcgatgaac acgaccgggt tgtcgtcgcg
     9661 gatcgccgtc ttgagtagcc ccttcgcgtc gtacgccgtc gacggcatca cgaccttcag
     9721 gccgggtacg tgcgcgtagt agctctcgag cgactgactg tgttgcgagc cgacctgcac
     9781 cgcagggccg cccgggccgc ggaacacgat gggcacgtta tattgcccgc cgctcatctg
     9841 ataaatcttc gccgcgttgt tcacgatctg atcgatcgcg acgagcgaga agttgaacgt
     9901 catgaactcg atgatcgggc gcaaaccgac catcgcggcg ccgacgccaa tgccggcgaa
     9961 gcccatctcg gcgatcggtg catcgacgac gcggcgctcg gagaaccgct gcagtaggcc
    10021 ctgcgtgacc ttgtacgcgc cgttgtagta gccgacctct tcacccatga tgaagacgtc
    10081 cttgtcgcgc tccatctcct cgcgcatggc ttggttgagc gcttcgcgat agctgatgac
    10141 cgacatcgct tactccttca ccacgtaggg gaggtattcg tcggccggcg gtgattcctc
    10201 cgcgaacttc acggcgtctt cgatctcggc tttcacgtcc gcctcgagcg aagtgagctc
    10261 gtcttctgtc atgccgagct cggtgaggcg cgcgcgcgag cggttgagcg gatcgcgccg
    10321 cttccactct tcgacctcgt ccttcgtgcg gtaaaggcca gggtcgctca tcgagtgacc
    10381 gcggaagcga tacgtcacga cttcgacgag cgttggctcg ctgctctggc gtgcgcgctc
    10441 gaccgcctcg gcgatgcggc gcttcacctt gacgacgtcg tcgccatcga accgatcgcg
    10501 cgccatgccg tgcgcgagtg cgcgcatcga cacgtcctcg accgcgagcg agcggtagag
    10561 cggcgtgccc atcgagtact ggttgttctc gcagatcagc acgaccggca gcttccacaa
    10621 gccggcgagc gcgagcccct cggcgaagcc gccgatcgtc gatgcgcctt cgccgaagaa
    10681 acacagcgtg accgcctggt cgccgcgata cttcgacgcg aatgccgcgc ctgccgcgat
    10741 cggcacgtgg ccgccgacga tgccgtagcc gccgaggaag ttcttgctct tgtcgaagat
    10801 gtgcatcgag ccgccgaggc ccttcacgca tccggtcttc ttgccgtaga gctcggcgag
    10861 aactgcgcgc gcgctcacgc ccttcgcata cgcatgacca tgctcgcgat acgtcgcgac
    10921 gacgtagtcc ttcggctcga gcgccgcgac cgcgccaacg cacaccgctt cctgaccgat
    10981 gatcaggtgg aggaagccgc cgatcttgcc ttgcgagtag gccttcgccg acgcctcttc
    11041 catgcgccgg attgccagca tctgccggta gagaggacgc agttcttcga ccgtggtctc
    11101 gactgccgat tcctcgggct cgatgcgttt cgtttcgggg gtgctcatgg acgccgtcgc
    11161 cacgggccga ccatacccat gaagcttgta aggtcaaatg attagggggc gagatcgaac
    11221 gacgcgttgc cctgtgcgtc aaacgcggtc acttcgctaa atccgccatc cacaccgagc
    11281 tccacggaca cgaagcggag cgggagcttg tcgatcgaaa acagccccgc tttgcacgga
    11341 actggttcat agccgaacgt gtcattgacg ctcgtcccgg cgaacatcag gtagaagttc
    11401 ggcgtcttcg cgcacagcat ctcgttcgcg ggcatgccgc ggatcgtcca ggtgacgcgg
    11461 atcgaccgca cttcgctggc cggcagacag tcgctgtttc gcgcgcaaac gaggttgtgg
    11521 ccgcagtcgc tgtcgacatg gcactcatcg ccgccgcccg ggcagccggc caacacgagc
    11581 acgagcgttg cgaatcgcaa catcagtagg gcaggtcgag gaccgcgttg ccttgcgcgt
    11641 caaaggtcgc gctgtcgccc cccgaggtgt cgcccgtgcg cgcgagcgtg acgtacgtgt
    11701 agcgcgccgg catcttgtcg atcgtgtacc gacccgcgtc gcacggcacg ggcgcgaagc
    11761 cgaacatgtc gaagctgtct gcgctcgtcg agaacgtgat gtcgagcgac ttcgagctca
    11821 cgcacgtcgc gtcgctcgcg acctgctgct tcaccgtcca gttcgcatgg atgatgcgca
    11881 cgcccgacgc gatcgtgcac tcgccgttgc gcgcgcagac ataaccgctg ccgcacgcgg
    11941 tatccgaatg acagccgtag ccggtgctgc cgccgctgcc gccccaccca cttccccaac
    12001 cgtcgccact accgccgccg ggatcgggac ccgggtcagg aggctcgatg atgcagccag
    12061 cgagcaagag cgcggcgagc gcgaaaggcc gcggcccacg ggccgcgccc tttcgactgg
    12121 tgacgcgggg cctcatgccg gtaatggtag ctcactcgac atagatcgcg ctcgtgtaga
    12181 tccatggcag ctcttgatcg gcccacgccg taccgaggtc gcgcaagtac ggcccgaggt
    12241 ggcgcggaac aatcgatact tcgacgcgat acgcccccgg tccgggtagc tcgacgtcga
    12301 gctcggggcc tgacccggat gcaaccgtcg tcacggcccc tgcgctgcag cgaatcacgg
    12361 tggccttgat ctccggagct ggcaaagtcg gatcgaggcc acgtaccgtg ggcaccttca
    12421 cgagcagatg cgtcgcgttg aacgtgaccg tgtcgccgag ctcaccgccg cccgtcgcga
    12481 ccacgtcgaa gccatcgggc gtgcccatga tctcgaacac gctgaacagc cggccctttg
    12541 cgagcgccgc ctcgatctgc gtgatgtcct tcggatcgtt caccagcacg atgttcgaga
    12601 accagcgcag cacgcgccgg tacgaatcgc cgcgctcgcc gtccgccaat atcaccggaa
    12661 gtgcgttttc gtgcgcatcg ctgccggccg tcgctggaat gtgcttgccc tcggcgagta
    12721 gctcgttcca gtgcgtgatc cccggcaggt tcggggtcaa gaacgacaac agggcgagat
    12781 ccggctcggg cccaccttcg ttcgtatcgg cgaactcgac cgcgttggtg atcgcgccat
    12841 agccgtcgag cccgagaaag tcggcgcgga tcttcggatc gatgttcgcg tgcaggttgt
    12901 agagctcgac gccgttcggc gcgaccgtac gcagctcgtc ggtcggatgt tgctcggtgt
    12961 gcgcgaccca aatcagtgcg cccgcatcac gcatcgcctg gatcgcagtc gccgatgtgc
    13021 cgttatagat gtcgtgccgc tcttgcacgg tgccggccac gtgatgatcg agcatgatcg
    13081 gcatcaagtc gttttcgccg ccgatcgtga tcagcacttc gtggccatcg tcgcagtgaa
    13141 tgcgggatgc gatctgattg ctgccgctca tcacgggcgt gtcggtgccg cgcatgctga
    13201 acagcgtcgt gaactcttcg tccgccatcg tgtcgtcgtg gtcggtcagt gccgcgaagt
    13261 cgatctgctc tgtgcagagc gccgcgcgca gatgctcgag gcacggctcg ttcggcgcgc
    13321 cggtcgcatc gcgtggcatg ccatcgcacg cgtcgtgcga atacggcgag tgcaggtgca
    13381 cgatgccgcg cgccggc
//
LOCUS       DASUFX010000383         8883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1034673, whole genome shotgun sequence.
ACCESSION   DASUFX010000383 DASUFX010000000
VERSION     DASUFX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034673"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFV99_15365"
     CDS             <1..1257
                     /locus_tag="VFV99_15365"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5060747.1"
                     /translation="DTIRARDGELGAFVEVAARRALMQARRADLMLRARGNRPGGPPF
                     LGVPTAIKDHEPVRGLGTRIGSRAFRWLVSPIDGYVARAMRRGGFIIIGKTTCSELTI
                     LPFVHVALHAPTRNPHDTSRYGGGSSGGAAVAVAAGMLPIAPGSDGGGSIRIPAAFCG
                     LVGFKTGRGTVPNPYGMLDAVGISSLGPLARTVRDAAAMIDTLAVRPDGSFARAVDGH
                     VPPLRVKVLLRSPLADVDVAPEHAAAATAVGRKLAELGHGVAEATPFVGQLDDFLPIM
                     ARMVARVPLLPGMVRLLEPSTQWLRERGRGVTRGDVVAVGERLSRDVLTWFGDADIVV
                     SPTVAQPAPKVGAYLNLDGEGVFRTAAAIGAFTAPFNVSGQPAISIPWGTTATGLPIG
                     VQLVGRRGTDRQLLALAARLTGENGV"
     gene            complement(1521..2549)
                     /locus_tag="VFV99_15370"
     CDS             complement(1521..2549)
                     /locus_tag="VFV99_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060748.1"
                     /translation="MRLLAYSLLLISSHAFAGSTIDPPSAPDAGTLRGNVLVWHDAML
                     YAEASDTASSIQLATIDGARKDHLGHVLALKVVGTKGAFVEVELTGQQDCAPSRVVVP
                     DDLARVRLFVRRADLAPVLVKSVTQSFDDGTSISLAPGTPVLPTDAGTYVVSLRSDAL
                     EVEVPSASVGFAYGVGKSRVSVMQGQTSAIAAATKVTLGARTLALTAWQGAPIERRGD
                     STLVALDGGCVSAHVLVPSKALADADESTGDLSASNDGNDVVSLRDECFLPKLTPLAI
                     GSKQVALAAKPIYLHAEPMGKNACIQRALRIESTLEIKSTDEKLRLCAPATKVLHEQL
                     RSARSAPR"
     gene            complement(2623..3816)
                     /locus_tag="VFV99_15375"
     CDS             complement(2623..3816)
                     /locus_tag="VFV99_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060749.1"
                     /translation="MGLDEPVSSVDQEASASPPWKQPAKWPYSIETPRVVLHYQATGD
                     LATAQTVLSDVEDAWTKQITQQGSRPPLDDGGVAGPDGRFDVYLQRGLDSLYVSSVAA
                     NAATPYDDYSTAMVLDPWGQYGGAEMRANVFHEFRHASQGADDWWEHIEFFEAEATAW
                     EAAYYGYDRLPYVWADYQAHPEWTPFKNDKYVTWFMYGGGLFLLHLKKSVFQNSFAFL
                     NETWLRSRSAGTNEPDFADALGIVLAAKGTTLFDQIVAFQRARWYTGSRANATIIEGA
                     AAIAEVATRSHTRASGAQRTSFVANPELLGTIYTVVTAAPTDGGTLRISLSNVDAKAR
                     PIVQVVGQNNTDRVLDLSSGSATVSVVGGKVVLAVTMLPSNGQFDPDSVGTQLYKGTV
                     NIDKM"
     gene            3921..4784
                     /locus_tag="VFV99_15380"
     CDS             3921..4784
                     /locus_tag="VFV99_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5060750.1"
                     /translation="MNPLVILGCGYVGTRIAKAALAQGRTVRVCGRSTGRMQPLGALG
                     ADVKFLDATTPKNFTSAMSSMAGSTVVYSIPPVTALPPGHAIRAALQAAYGNGALYFV
                     HFSSCGLYGDEPDDDVWIDEDTPTRPDAAMQNIMSDEVEVERAAFERMRTVVLRLAPV
                     YGPGRGVRNRIRKGDYRILDEGQHATSRIHVDDVARIVLAVEGNAPNKSIYLVGDDEP
                     TTQGEYAKWLSERMGVPLPPYRAMIEPGKPKVAHRNRKIRNAKLKRELGIELLYPSYR
                     QGEEAIEKEEA"
     gene            5546..5626
                     /locus_tag="VFV99_15385"
     tRNA            5546..5626
                     /locus_tag="VFV99_15385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5579..5581,aa:Leu,seq:tag)
     gene            complement(5635..6810)
                     /locus_tag="VFV99_15390"
     CDS             complement(5635..6810)
                     /locus_tag="VFV99_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5060751.1"
                     /translation="MVQKPDGTRERISGSAPEYINTKVAAERAMLDHIERVMHPERVP
                     TRKETPSFSDWFRGRFWKEWVVGRMNKPTEVRSKNIIFECHLEPRFGKMPLDEVTTSE
                     VAQFRADLVAKGLSKKRINNILAVLSKPMKYAVDCEVLVKAPKIGMFKVERPEIVAWD
                     FEEYARMLKAAKEEGEEWYAAACLAGEAGLRVGEVKALRWRDDVDLIARTITVNLQVR
                     NKETTTPKGRKRRTIPMTSTLHEALKRMSVIREGLVVRNLDGTAKSDDQADKAMGRIC
                     RRAGLPVRLFHTMRHTFGTHAALFGVNPWRLQAWLGHKRIDETMLYVHVAEAHPRQPP
                     KHVQKAGRRISDPDARVLAMLGARGAESSSEATPKPMRRGKGVAKVQALDLKVLKAS"
     gene            complement(6858..7298)
                     /locus_tag="VFV99_15395"
     CDS             complement(6858..7298)
                     /locus_tag="VFV99_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5060752.1"
                     /translation="MSRASAKQRAPTDAAANANEAALALSTETRDDADLGWLRSRELM
                     DRIAVEVFKGVVTAVQGALRGETLLPQPTAPAPPTSEPATDVMTADEVAEFLGLDRNT
                     VYDFAGRGEIPHQRLGKRLLFRRGALVSWLDASLCKATLTRKAR"
     gene            complement(7295..7750)
                     /locus_tag="VFV99_15400"
     CDS             complement(7295..7750)
                     /locus_tag="VFV99_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060753.1"
                     /translation="MTNTNDWRQAVEDEAQRLEAGAGLMRLIAMAEDQIDAIRVISDL
                     MAAVWHCDKARAAREHRHLALNPPIPVSKLLTNFAESLREKLVTVAGLLPQEHRDRLL
                     AIAPAVQASLEAQEPDVVELVAKYLAAPPDAIAFAIIFCIDAVEQRFAS"
     gene            complement(7782..>8883)
                     /locus_tag="VFV99_15405"
     CDS             complement(7782..>8883)
                     /locus_tag="VFV99_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060754.1"
                     /translation="NSHPPLARDPQGHLVELPKGTCAWRIGRETTGRPRIILAPDNQP
                     MRFPLHLTSEQLVEMCGLDVYHVYAIDEFGNMLGRVTKLDATRDARELRNGFAGDVPT
                     APTSRTPYGQMTDLRFALEAMAQMMRTNSEALRAVTESQADWVKAIAVAKGMPRNVAF
                     PSEPAPAESYDYDDDDEAEEQEGTSVAGAATNVYDVVNKAIDKFGDFVPGLLTSNPAA
                     SPKKAAPRNAAGKAQASDIDWDLVYAPNWEARDAIDLDYAHRKGKAKRAAKALEQEAA
                     TTPRLTLHQQLARDPALQQRLMAIKAHITPEEAEVVINAVMASPPEQQDDFLSKVRSL
                     SVEEGAEMCRVVAADVREHQAGKQTPSDGAAA"
BASE COUNT         1453 a         2974 c         2846 g         1610 t
ORIGIN      
        1 gacacgattc gcgcccgcga tggcgagctc ggtgcgttcg tcgaggtcgc agcacgccgc
       61 gcgctgatgc aggcgcgccg cgcggatctc atgctgcgcg cgcgcggcaa ccgacccggc
      121 ggtccgccgt ttctcggtgt gccaaccgcg atcaaggatc acgaaccggt gcgtggcctc
      181 ggcacgcgca tcggctcgcg cgcatttcgt tggctcgtat cgccgatcga cggctacgtc
      241 gcacgcgcga tgcggcgcgg cggcttcatc atcattggca agacgacgtg ctcggagctg
      301 acgatcctgc cattcgtgca cgtcgccctg cacgcaccga cgcgcaaccc gcacgatacg
      361 tcccgctacg gcggtggctc gagcggtggc gcagccgtgg cggtcgctgc tggcatgcta
      421 ccgatcgccc ccggctccga cggtggtggc tcgatccgga tccccgccgc gttctgcgga
      481 ctcgtcggct tcaagaccgg ccgcggcacg gtgccgaacc cgtacggcat gctcgacgcc
      541 gtcggcatca gctcgctcgg tccgctcgcg cgcaccgtgc gtgacgccgc cgcgatgatc
      601 gatacgctcg ccgtccgtcc cgacggctcg ttcgcgcgcg ccgtcgatgg ccacgtgcca
      661 ccgttgcgcg tgaaggtcct gctgcgctcg ccgctcgctg atgtcgacgt cgcgcccgag
      721 cacgccgcgg ctgcgactgc ggtcgggcgc aagctcgccg agctcgggca cggtgtagct
      781 gaagcgacgc cgttcgtcgg ccagctcgat gacttcttgc cgatcatggc gcgcatggtc
      841 gcgcgcgtgc cgctgttgcc cggcatggtg cggctgctcg agccctcgac acagtggctg
      901 cgcgagcgcg gtcgcggcgt gacacgcggt gatgtcgtcg cggtgggcga gcgactgtcg
      961 cgcgacgtgc tcacctggtt cggcgatgcc gacatcgtcg tgtcgccgac ggtcgcgcag
     1021 ccggcgccga aggtcggtgc gtacttgaat ctcgatggcg aaggtgtgtt ccggaccgcc
     1081 gccgcgatcg gtgcgttcac tgcgccgttc aacgtcagcg ggcagcccgc gatctcgata
     1141 ccctggggca cgaccgcgac gggcctgccg atcggtgtcc agctcgtcgg ccgtcgcggc
     1201 accgaccgcc agctactcgc gctcgcggcc cggctcaccg gcgaaaatgg ggtctgaccc
     1261 cttttctgtt cgccgacgtt gcacacggcc gtttgcagcg cgcggtactc ccgttcgtga
     1321 actcacggac ttgggtgcga aagacaccgg aacgttcgtt gcacaaacga agttcatgcg
     1381 tcgtttcccc cgccccggcc gcaccgagat ccggcttggt aaccggctcg aggccgtcag
     1441 cggcgagatc gtgacgcgga cgcacctggc cacggggacc ggtgcctacg aaaccgttgg
     1501 tgcctacgaa acgatcgaga ttatcgcgga gccgagcgcg cactacggag ctgctcatgc
     1561 agcaccttcg tcgccggcgc gcacagacgc agcttctcgt ccgtcgactt gatctcgagg
     1621 gtgctctcga tgcgaagcgc gcgctgaatg cacgcgttct tgcccatcgg ctcggcgtgc
     1681 aaatagatcg gcttggcggc aagcgcgacc tgcttgctgc cgatcgcgag cggcgtgagc
     1741 ttcggcaaga agcactcgtc gcgcaggctg acgacgtcgt tgccgtcgtt gctcgcgctg
     1801 aggtcgccgg tgctctcgtc cgcatcggcg agcgccttgc tcggtaccag cacgtgcgcg
     1861 ctcacgcaac cgccgtcgag agcgacgagc gtcgagtcgc cgcggcgctc gatcggtgcg
     1921 ccctgccatg cggtcaacgc gagcgtgcgc gcgccgagcg tcaccttcgt cgctgcagcg
     1981 atcgcggacg tctgtccctg catcacgctc acgcgcgact tgccaacacc gtacgcgaag
     2041 ccgaccgagg cgctcggcac ttccacctcg agcgcgtcgc tgcgcagcga gacgacgtac
     2101 gtgcccgcat cggtcggcag cacaggcgtg ccgggcgcga gtgagatcga ggtgccatcg
     2161 tcgaacgact gcgtgaccga tttgacgagc accggcgcga gatccgcgcg gcgcacgaac
     2221 agccgcacgc gtgcgagatc gtccggaacg acgacgcgcg atggtgcgca atcctgctga
     2281 ccggtgagct cgacctcgac gaaggcgccc ttcgtgccga cgaccttgag cgcgaggacg
     2341 tgaccgagat gatccttgcg cgcgccgtcg atcgtcgcga gctggatcga cgacgccgta
     2401 tccgacgcct ccgcgtacag catcgcgtcg tgccacacga gcacgttgcc gcgcagcgtg
     2461 ccggcatcgg gtgcgctcgg cggatcgatc gtggagcccg cgaacgcatg cgagctgatc
     2521 aatagaagcg aataagctag gagccgcatt tgcgcggcgg aacctgccac gaccgcgccg
     2581 gtcatgtggt ggcaaatctc gtcctgaggt gcgttcagga cgctacatct tgtcgatgtt
     2641 tacggtgccc ttgtagagct gcgtcccgac gctatccgga tcgaactggc cgttcgacgg
     2701 cagcatcgtg acggcgagca cgaccttgcc gccgaccacg gacacggtcg ccgagcccga
     2761 cgacaagtcg agcacgcggt ccgtgttgtt ctgaccaacg acctgaacga tcggccgcgc
     2821 cttcgcgtcg acgttcgaga gcgaaatgcg aagcgtgccg ccgtccgtcg gcgctgccgt
     2881 cacgaccgta tagatcgtgc ccagcagctc gggattcgcg acgaacgacg tgcgctgcgc
     2941 acccgaggca cgcgtgtgcg agcgggtcgc gacttcggcg atcgctgcgg cgccttcgat
     3001 gatcgtggcg ttcgcgcgcg acccggtgta ccagcgcgca cgctggaacg cgacgatctg
     3061 gtcgaacagc gtcgtgccct tcgcggcgag cacgatgccg agcgcgtcgg cgaagtcagg
     3121 ctcgttcgtt cccgccgaac gcgagcgcag ccacgtctcg ttgaggaacg cgaagctgtt
     3181 ctggaacacg gactttttca gatgcagcaa gaacaagccg ccgccgtaca tgaaccacgt
     3241 gacgtacttg tcgttcttga acggcgtcca ctcggggtgc gcctggtaat cggcccacac
     3301 gtacggcagc cgatcgtagc cgtagtacgc ggcctcccac gccgtcgctt cggcctcgaa
     3361 gaactcgatg tgctcccacc aatcgtccgc gccctgcgat gcgtgacgga actcgtggaa
     3421 cacgttcgcg cgcatctcgg cgccgccgta ctggccccac ggatcgagca ccatcgcggt
     3481 cgagtaatcg tcgtagggcg tcgcggcgtt tgcggcgacg gaggagacgt agagtgaatc
     3541 gaggccgcgc tggagataga cgtcgaaccg accatccggg ccagcgacgc cgccgtcgtc
     3601 gagcggtggc cgcgaacctt gctgcgtgat ctgcttggtc cacgcgtcct cgacgtccga
     3661 gagcaccgtc tgcgcagtcg cgagatcgcc cgtcgcctga taatggagca ccacgcgcgg
     3721 tgtttcgatc gagtacggcc acttcgccgg ctgcttccac ggcggcgatg ccgacgcttc
     3781 ctgatcgacg gacgacaccg gctcgtccag gcccacgcac ccactgagaa tcgctacaac
     3841 gacaacgcct ctgaccatag tccgcgcaca gtagtcgagc ggtggcacga gtcaacagca
     3901 attcgagcta cattgcggcc gtgaacccgc tcgtgatcct cggttgcggc tacgtcggca
     3961 cgcgaatcgc gaaggctgcg ctcgcacaag gccgcaccgt ccgcgtctgc gggcgctcga
     4021 cggggcgcat gcaaccgctc ggcgcgctcg gcgccgacgt gaaattcctc gacgcgacga
     4081 cgccgaagaa cttcacctcc gcgatgagca gcatggccgg ctcgaccgtc gtgtactcga
     4141 tcccgccagt cactgcgttg ccgcccggtc acgcgatccg cgccgcactg caagctgcgt
     4201 acggcaatgg ggcgctctac ttcgttcact tcagctcgtg tggcctgtac ggcgacgagc
     4261 ccgatgacga tgtgtggatc gacgaggaca cgccgacgcg tccggacgcc gcgatgcaga
     4321 acatcatgag cgacgaagtc gaggtcgagc gcgccgcgtt cgagcgcatg cgcactgtcg
     4381 tgctgcgtct cgcgccggtc tatggcccgg gccgcggcgt gcgcaatcgc atccgcaaag
     4441 gcgattatcg gattctcgac gaagggcagc acgcgacgtc gcgcattcac gtcgatgacg
     4501 tcgcgcggat cgtgctcgcc gtcgaaggga atgcgccgaa caagtcgatc tacctcgtcg
     4561 gtgatgacga gccgacgacg caaggcgagt acgcgaagtg gctgagcgag cgcatgggcg
     4621 tgccgctacc gccttatcgc gcgatgatcg aacccggcaa accgaaggtg gcgcatcgca
     4681 atcgcaagat ccgcaacgcg aagctcaagc gcgagctcgg catcgagctg ctctatccgt
     4741 cgtaccgcca gggcgaagaa gcgatcgaaa aagaagaggc ctgagcgtca gagggcatcg
     4801 ctacgatcga tctatgcgtc gatcaaaata tgatgctgcg acgctcgcgc caatcgtggc
     4861 gagctcgcag tcgcttgcgg atgtcattcg caacctcggt ttgcgaccca ctggtggaaa
     4921 ccaccggtac atcaaggctc gaatccgaca agcaggcctc gacacatcgc acttcggctg
     4981 gggcaaacta cgtgcggccg tcgagcgagt gtcggcggaa gacctccgag cgctcatcga
     5041 agagtcgacc tcattcgcgc aaacactcgt aaagctcgat ctgccaacgg taggacgtgc
     5101 gcaccacgag ctgaaaaagc gcgtccgcaa tctcgggatc gcaactgacc actttaccgg
     5161 tccaggttgg tcgcgcggca aaacctcgcg aacccatccg agcatcgcgc gcgttgcaaa
     5221 ggcgcatacg cgatccgacg atgaggtctt catcgagaac tcgccggaga caaaaggacc
     5281 cgcgattgca cgtaggttgg tcgcaaaagg ttggtcgtat gcgtgcgctc tttgcggcac
     5341 ttcgcaatgg atgggaaagc cgctcgtcct acatctcgat cacatcaacg gcgtccacaa
     5401 cgacaaccgt tacgtcaacc ttcgtctcct gtgcccgaac tgccattcgc agacggacac
     5461 gtactgcaat cgtcgacgac cacagccgtc ccgggcgtca gagccgcgcg cgcgttactc
     5521 gtgctatacg ttccgcgcat cacacgcgag cgtggtgaaa tggcagacac gacagtctta
     5581 ggcgctgttg gggtaacccg tgggggttcg agtccccccg ctcgcactct taacttacga
     5641 agctttcagg actttcagat ctaaggcctg aacttttgcc acgcctttgc cacggcgcat
     5701 cggtttcggc gtcgcttccg acgacgattc cgcgccgcgt gcaccgagca tcgcgagcac
     5761 gcgcgcatcg ggatcgctga tgcgacgccc tgccttctgc acgtgcttcg gcggctggcg
     5821 cgggtgcgcc tccgccacgt gcacgtagag catggtctcg tcgatgcgtt tgtggccaag
     5881 ccacgcctgc aagcgccacg gattcacgcc gaacagcgca gcgtgagttc cgaacgtgtg
     5941 ccgcatggtg tggaacaaac gaaccggcaa gccggcgcgg cgacagatgc gccccatcgc
     6001 cttgtcggcc tggtcgtcgg acttcgccgt gccgtccagg ttccgcacga cgagcccttc
     6061 gcggatgaca ctcatccgct tgagcgcttc gtgcagcgtt gacgtcatcg ggatcgtccg
     6121 ccgcttgcgg cccttgggcg tggtcgtctc cttgttccgg acctgaaggt tgacggtgat
     6181 ggtcctggcg atcaggtcga cgtcatcacg ccacctgagc gctttgacct cgccgacgcg
     6241 tagccccgcc tcaccggcaa gacacgcagc ggcgtaccac tcctcgccct cttccttcgc
     6301 tgccttgagc attcgcgcgt actcctcgaa gtcccacgcg acgatctccg gccgctccac
     6361 cttgaacatg ccgatcttcg gcgccttcac tagcacctcg caatcgaccg cgtacttcat
     6421 cggcttcgac aacaccgcga ggatgttgtt gatgcgcttc ttgctcagcc ccttcgccac
     6481 gaggtcggcg cgaaactgcg cgacctcgga cgtcgtgacc tcgtcgagag gcatcttccc
     6541 gaaccgcggc tcgagatgac attcgaagat gatgttcttc gagcgcacct cggtcggctt
     6601 gttcatgcgg cctacaaccc attccttcca gaaccggcca cggaaccagt cactaaacga
     6661 cggtgtctcc ttcctcgtcg gcacgcgttc cggatgcatc acgcgctcga tgtgatcgag
     6721 catcgcgcgc tccgcggcga cctttgtatt gatgtattcg ggcgccgagc cactgatgcg
     6781 ctcgcgcgtt ccgtccggtt tctggaccac gtggcgataa cgccaacgcc cttcggatgt
     6841 acgtcgaatc ggcatagcta tcttgctttc cgagtcaacg tggccttgca tagtgacgcg
     6901 tccaaccatg acacgagcgc gccgcgtcgg aacagcaaac gtttgccgag gcgctggtgt
     6961 gggatctcgc cgcgaccggc gaagtcatag acggtgttgc ggtcgagccc gagaaactcg
     7021 gcaacctcgt ccgcggtcat gacgtcggtg gccggctcgc tcgtcggcgg cgccggcgcg
     7081 gttggctgcg ggagcagtgt ctcgccgcgg agcgcaccct gcaccgccgt cacgacgcct
     7141 ttgaagacct cgaccgcgat ccggtccatc agctcacgcg agcgcagcca gcccagatca
     7201 gcgtcgtcgc gcgtttcggt cgagagtgcg agcgcggcct cgttcgcgtt cgctgcggca
     7261 tccgtgggcg ctcgttgctt cgcgctggcg cggctcacga cgcgaacctc tgctcgaccg
     7321 cgtcgatgca gaagatgatc gcgaaggcga tagcgtcggg tggtgctgcg aggtacttcg
     7381 ccacgagctc gacaacgtcg ggttcctgcg cctcgagcga ggcctgcacg gcaggtgcga
     7441 tcgcgagcag acgatcgcgg tgctcctgcg gcaacaagcc ggcgacggtg acgagcttct
     7501 cgcgaagact ctcggcgaag ttcgtcagca gcttcgagac gggaatcggc ggattcagag
     7561 ccaagtgccg gtgctcgcgt gcagcacgcg ctttgtcgca gtgccacacc gctgccatga
     7621 gatccgagat gacccggatc gcgtcgatct gatcctcggc catcgcgatg agccgcatga
     7681 ggcccgcacc tgcttcgagg cgctgggcct cgtcctctac tgcctgtcgc caatcattcg
     7741 tgttcgtcat gttcgttctc ccctacgtgg tggtgatgcg actacgccgc cgctccgtcg
     7801 gacggggtct gtttccctgc ttggtgttcc ctcacatcag cagcgacgac gcggcacatc
     7861 tccgcgcctt cttcgacgga gagcgatcgc actttcgaga ggaagtcgtc ttgctgctcg
     7921 ggcggtgacg ccatcactgc gttgatcacg acctcggctt cctcgggtgt gatgtgcgcc
     7981 ttgatcgcca tcaagcgctg ctgaagcgca ggatcgcgag cgagctgctg atgaagtgtc
     8041 agcctcggtg tggtggctgc ctcttgctcc agcgccttcg cggcgcgttt cgccttgcct
     8101 ttgcggtgtg cgtagtcgag atcgatcgcg tcgcgcgcct cccagttcgg cgcgtacact
     8161 agatcccagt cgatgtcgct cgcctgcgcc ttgccggctg cgttgcgcgg cgccgccttc
     8221 ttcgggcttg ctgcgggatt gctcgtgagc aagccgggga cgaagtcgcc gaacttgtcg
     8281 atcgccttgt tgacgacgtc gtagacgttc gtcgctgcgc ccgcaacgga tgtgccttcc
     8341 tgttcctcgg cctcgtcgtc atcgtcgtag tcgtatgact cggccggcgc tggttccgat
     8401 ggaaatgcca cgttgcgcgg catgcccttc gccaccgcga tcgccttgac ccaatcagcc
     8461 tgtgactcgg tgaccgcgcg caacgcttcg ctgttcgtgc gcatcatctg agccatcgcc
     8521 tcgagcgcga agcgcaggtc ggtcatctgg ccgtatggag tgcgcgaggt cggcgctgtt
     8581 ggcacatcgc ccgcgaagcc gttgcgtagc tcgcgggcat cacgcgtcgc atcgagcttc
     8641 gtgacgcggc cgagcatgtt cccgaactcg tcgatcgcgt acacgtgata gacgtcgagc
     8701 ccgcacatct cgacgagctg ctcgctcgtg aggtggagcg ggaagcgcat cggctggttg
     8761 tcgggcgcga ggatgatgcg cggtcggccc gtggtctcgc ggccaatgcg ccatgcgcac
     8821 gttcctttag ggagctcgac aaggtggccc tgcgggtcgc gcgcgagcgg cggatggcta
     8881 ttg
//
LOCUS       DASUFX010000384        13412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_895763,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000384 DASUFX010000000
VERSION     DASUFX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895763"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /gene="pruA"
                     /locus_tag="VFV99_15410"
     CDS             <1..1396
                     /gene="pruA"
                     /locus_tag="VFV99_15410"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX5060755.1"
                     /translation="TAERAIRAALDARREWSNMRWESRAAVLLRAAELLSGPWRMRMN
                     AATMHGQSKTCHQSEIDAACEMIDFWRFNAAYAQQLYQMQPKSAPGMWNMSELRPLEG
                     FVFALTPFNFTAIGGNLPTAPAVMGNTVVWKPAESQLLAAWRTFQIFEEAGFPPGVIN
                     FIPGLAHDKTNIILDHPELAGIHFTGSTDVFRMMWKRVADNIDRYKNYPRLVGETGGK
                     DFILAHASADPKAVATAIIRGGYEYQGQKCSAASRIYVAQSVWKAMKDELISEIGTIK
                     VGDVSDFSNFMGAVIKKTAFDKHAAAIAEARETKGMKVLAGGTCDDREGWFVQPTLLQ
                     TDDPNVRHMRDEFFGPIVTLHVYPDTAWEETLKLVDRTSPYALTGGVFARERTAIVEA
                     TAALRDAAGNFYINDKPTGAVVGQQPFGGARASGTDDKAGSPLNLLRWTAARTLKETF
                     VPPTDYRYPFLNES"
     gene            1531..4260
                     /locus_tag="VFV99_15415"
     CDS             1531..4260
                     /locus_tag="VFV99_15415"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5060756.1"
                     /translation="MNRKVGALVAAVLVAALAIWFFAFRDRSSSKPQPKAGRNGKIEL
                     PKSTPAKTDDTPAPQGMAPRWSLDVDPEGPLRLEGQVVDEDGHGVGGAEVWLGSVPPR
                     SAKSEDDGTFAFDKLVGREYGLTATAGERVGGPVQYKLTESSDPVVIRLTKSAKVVVT
                     VLADDSKPIEGADVRLAAMGERTAKTAADGTATLSPVAPGWVAVQATANGYAPGNGFT
                     QVGSAGAVGTIKVTLHKGVPVTGRVVDEAGAPIAKAHMSTAGIWDLPSATGCATCNQV
                     VTDTNGEFTFPALGAGTHVLVATDGEHAPARSAPVTVADQPVKNVKITMKAGGTLAGT
                     VVDDQQKPVPFATVRVGGDGQQMWMVDARQATSDRTGHFELKGLARAKLKARAESDIA
                     ASAIATLDLTSTPKAELKLVLDVKGTIAGVVVNETNEPVAEVQVNAFPDIFGGNSPES
                     ISLAGFSSATTDGAGHFVIRGLPDGGYRLRAARSTGGRYDWGQQGTPAKVGDKNVKIT
                     LAAEGSIVGKLVVEGGGAPKLATVQVGSQPSTPASADGTFKLEDVAPGKYDLHVRGHD
                     FAEFIQHDVEVKPGKPTDVGTLTLMRGRKLVGKVIDAGGNPVPGTKVKTGDMLFSMQG
                     GDDQMENLEEMYGARTDITDQDGKFTLIGIAKKSTSVIADHPTRGRSVAVSLPAGTDD
                     PPPVTLTLKGYGSITGKVTSKGEPVGGATITDTPKGGGAQIRIVQSEADGTFTLTKIG
                     EGTHVVSAMQQSMLGMSLKSTSTTVQVREGKESKVAIDIPVGSLVLAVQVKAQAGQKV
                     DSAQIFLFRGAIVVHNAKELTEGFLSGGVQGMKFWLGEGKPVPEFDELVAGDYSVCTI
                     PITGDLNDSQFQARIQEHMDVLKVYCKKVPLAASPQKQTFIAEVPAMTPLPQN"
     gene            complement(4263..4712)
                     /locus_tag="VFV99_15420"
     CDS             complement(4263..4712)
                     /locus_tag="VFV99_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060757.1"
                     /translation="MKRIILSALVALTAFGGVASADRGRGVRHSQGGVSVTHSRGRYV
                     QPRGHVTYNRGYVARPSYRYVRRPIYVQRPRIAYHYYNYYQRPALLVENYAAVPGYYW
                     VAGQWNWDGYEWIWTPGHYEPDPNAAGYYSNTYSSGYYANPYYDSGY"
     gene            complement(4780..5214)
                     /locus_tag="VFV99_15425"
     CDS             complement(4780..5214)
                     /locus_tag="VFV99_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060758.1"
                     /translation="MTLAVIGGVASADRHHGGRWQRGNNWSGGVRVTAPRVVVQPRVV
                     VQQPRRVYVQRTHVVRRPIFVTAPRIRYRYYNYYQRPAVLAENYAPMTGYYWVSGQWG
                     WTGYEWTWTAGHYEPDPNYVEPNYDYSSYDNGYDANCDHDRY"
     gene            complement(5399..7426)
                     /locus_tag="VFV99_15430"
     CDS             complement(5399..7426)
                     /locus_tag="VFV99_15430"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX5060759.1"
                     /translation="MALSACSDDLDGPGEHPIAKTTQLVQYQSCSALETDLKNMVIYE
                     IWADIDRANYWGWGQGGAEGDAAGGAGSGSGGGRQEGVDFSGTNNQEQGVDEADIVKT
                     DGYHIYTLNGNRLHIMGVPNFGELTAESVTKIEGYPQQMLLDSKANRVVVLSWIDTYS
                     LPDGHPLKQLVGYQDDQSGWHWRINQLSKITVLDITNKAAPTLVREVYYEGWYQTARK
                     IDSSIRIAGYSMIEPSIMWGWWNLYEQNGEDKTATKIAVRNYVNSLALADFIPQIYVR
                     NPNGQFTSNSLSEGSCRSFYRPTDSHARGISSIISFDLLGDTVHWDADHIVSNWSTFY
                     ASKDTLLLAEPAHDWWWYWWYQDDPDQLNVHAFDISTPGKSVYTGSGRVDGQIVDQFS
                     LDEDNGAIRVATTTGLLWRWWADDEERPTMENHVWVLEDHGSKLDIVGHVGGIAKGER
                     IMSSRFQGNKAYLVTYQYVDPLFTLDLSDRRNPHVVGELKVPGFSTYLHPIADGKLLS
                     IGVDGTNQWRTQVSLFDVSDFAHPALDAALPVEAQNTWGWSEALYDHKAFQYWAPKGL
                     LAIPQSTYAYQNTGNGQYYYRYLSQLVLVNVDPTSGALSIKGRIDHSPYYDAEGNTYW
                     RYTDIRRSIFMGDFVYAISDKAVTAHRVSDLGKTAEQTLPGYTPNDYYWWW"
     gene            7579..8478
                     /locus_tag="VFV99_15435"
     CDS             7579..8478
                     /locus_tag="VFV99_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060760.1"
                     /translation="MRLLAFVLAAAAGCGSSTLSGPCSLDSACSDGEVCDFTAEGGPI
                     CISADGDVDNDGIPNGEDFCEHAMGGRYDEDKDGVGDDCDKCPIAPPRDTPDSDGDGV
                     ESPCDPAPTEPGDEIIFFDGFNGALSADWKAMTPAAWTGGDGEVTANLSAVATEDSLS
                     RVVPGKTNVAVEIGYKVDRVENSSPRHMVAVYADDPRPAGTATVQCGVSRADSAAGDL
                     VFVQTNSGQMSSMTVDPALLTTKSYRASAYMSGARAGCVVIANNMALGTVQTTITPDD
                     LSTIALNAQSVTARFQYVLVVGH"
     gene            complement(8800..10554)
                     /locus_tag="VFV99_15440"
     CDS             complement(8800..10554)
                     /locus_tag="VFV99_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060761.1"
                     /translation="MTLAIAVLAGCGDDGHKVEADARLEGFDKPDLVCPGDPQCKTAG
                     DGVLKVGVAKRTYTPQNFETYTDENMDRQWQTTEPYTDLNGNGKFDGVWLFGGARAAL
                     GVTTDVEVRAMAFEEGDMRIVIAYVDCVGMFVGDMDSIREDPALASLGIDHIIIGSTH
                     AHDAPDTAGIWGPRVGESGREQFVIDKLHAAAVEAIAEAVNNVQPAQMIIASSKTIND
                     PTNEQSKTDDWWKDLRDPQIMDPTLTIARFVKVSNPSETIGTLVNWADHPEVAHFDDT
                     VPATITAHYPHWLRDHVENGVLTTESKYALQDLAGLGGITVFVQGALGGQIGSLRGTH
                     PPGPGGVPVTKTSHAMDQALGTNAAAVALTALRDKGESTSDLPLSFKSAKFYARVDNL
                     GIQVYFILGILGPHPTAGYNPDEPIDETNTPWLPLRATYVQIGPLGLVTTPGELHPEL
                     WVGGYNGEWSFGWPLLQHAGHCADNNNDCNEQQMCNTGVECVPTPNLPKFDEAPQPPY
                     MRDLVLAHDGVRYPVVAGLAEDYVGYIVPAYNFVLATTGPWLNEAEGDHYEEVYCLSK
                     DAERHIVDSTLALLKYRR"
     gene            complement(10619..11245)
                     /locus_tag="VFV99_15445"
     CDS             complement(10619..11245)
                     /locus_tag="VFV99_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5060762.1"
                     /translation="MRALLLVVLVACDTGGPPDYSAQLVAHYTMDSGTGGTVADSSGN
                     HYDGMCTVCPTAEAGKVGGALRFNGGDQQVDVPSLAAFDYVARGFSASAWVFVDMPPP
                     QLPGCPIIKGAMWSICVTPDMHPEFSSFEATSATLTAGEWHHIAISYDGKMKRIVLDG
                     VEAGTMAASILSENGQIILGGELIGVADDIRVYSGVLTDDEIADLVTP"
     gene            complement(11262..11912)
                     /gene="msrA"
                     /locus_tag="VFV99_15450"
     CDS             complement(11262..11912)
                     /gene="msrA"
                     /locus_tag="VFV99_15450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5060763.1"
                     /translation="MFFSTKKSRMPSPDEALRGRSTAMQVGDTHFVNGHRITPPFPDG
                     TQRALFGMGCFWGAERKFWETPGVYSTAVGYAGGFTPNPTYEEVCSGMTGHNEVVLVI
                     YDPKQVSYDKLLGVFWESHDPTQSMRQGNDVGTQYRSGIYTFSAEQQKAAEASKAMYQ
                     DRLRTAGHGDISTEIREAPPFYYAEDYHQQYLGKNPGGYCGLGGTGVSCPIGLGAK"
     gene            11959..12519
                     /locus_tag="VFV99_15455"
     CDS             11959..12519
                     /locus_tag="VFV99_15455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5060764.1"
                     /translation="MTGVSLLGICGSTRKASMNAVLLRTVAEALPHDAKLEIWEGLDH
                     LPIFNSDLPEPPGILALKGRIAAADGVIFSVPEYNYSIPGGLKNALDWVSRPPAMSPM
                     RGKPIGLVGAATGMSGTIRAQNHMRQILVYSDSPVMSQPEVLIPRAHERFIDGKLTDE
                     PTHQLLQRFATAFVAFVHRHKVLPGA"
     gene            complement(12615..>13412)
                     /locus_tag="VFV99_15460"
     CDS             complement(12615..>13412)
                     /locus_tag="VFV99_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5060765.1"
                     /translation="NGDADDLVYIHKGGGTLRSLLGDIEFAQGDYVFVPRGLLHRFLP
                     KGEQHWFWFSLNGGLHLPRQWRNEVGQLRMDAPYSERDFKRPKLQPPYDEKIRGMVVR
                     RDGKWHGFTLEHSPFDVVGWDGAVYPWAFPILAFQPRVSSVHLPPTWHGTFAARGALI
                     CSFVPRPVDFHPEAIPCPYPHSSTHCDEILFYADGNFTSRKGVGPGSISHHPIGVPHG
                     PHPGAYERSIGTKETTELAVMIDTFLPLHTTSAAVSVEDAAYHDSFV"
BASE COUNT         2361 a         4372 c         4386 g         2293 t
ORIGIN      
        1 caccgcggag cgtgcgattc gtgcagcgct cgatgcgcgt cgtgagtgga gcaacatgcg
       61 ctgggaatcg cgcgcggcgg tgctgctccg cgcggccgag ctgctcagcg gcccgtggcg
      121 catgcgcatg aacgcggcga cgatgcacgg ccagagcaag acctgccatc agtcggagat
      181 cgacgcggca tgcgagatga tcgacttctg gcggttcaac gccgcctacg cgcagcagct
      241 gtatcagatg cagccgaaga gcgcgcccgg catgtggaac atgagcgagc tgcggccgct
      301 cgagggtttc gtgttcgcgc tgacgccgtt caacttcacc gcgatcggcg gcaacctgcc
      361 gaccgcaccc gcggtcatgg gcaacacagt cgtgtggaag ccggccgaga gccagctgct
      421 cgcggcgtgg cgcacgttcc agatcttcga ggaagcgggc ttcccgccgg gcgtgatcaa
      481 cttcatcccc ggcctcgctc acgacaagac gaacatcatc ctcgatcatc cagagctcgc
      541 cggcattcac ttcaccggct cgacggacgt gttccgcatg atgtggaagc gcgtcgccga
      601 caatatcgat cgctacaaga attatccgcg cctcgtcggt gagaccggcg gcaaggactt
      661 catcctcgcg cacgcgtcgg ccgatccgaa ggcggtcgcg accgcgatca tccgcggtgg
      721 ctacgagtac cagggccaga agtgctcggc ggcgtcgcgc atctacgtcg cgcagagcgt
      781 gtggaaggcg atgaaggacg agctgatttc cgagatcggt acgatcaagg tcggtgacgt
      841 cagcgacttc tcgaacttca tgggcgccgt gatcaagaag accgcgttcg acaagcacgc
      901 ggccgcgatc gccgaagcgc gcgagacgaa gggcatgaag gtgctcgccg gcggcacgtg
      961 cgacgaccgc gaaggttggt tcgtgcagcc aacgctgctg cagaccgacg atccaaacgt
     1021 ccgccacatg cgcgacgagt tcttcggccc gatcgtgacg ctgcacgtct atcccgacac
     1081 cgcgtgggaa gagacgctga agctcgtcga tcgtacgagc ccctacgcac tgaccggcgg
     1141 cgtgttcgcg cgcgagcgca ccgcgatcgt cgaagcaacc gcggcactgc gcgacgcagc
     1201 cggcaacttc tacatcaacg acaagccgac cggcgccgtc gtcggtcagc agccgttcgg
     1261 tggtgcgcgc gcgtcgggca ccgacgacaa agccggttcg ccgctcaacc tcctgcgctg
     1321 gaccgcagcg cgcacgctca aagaaacgtt cgtgccgccg accgattacc gctatccgtt
     1381 cctgaacgag tcgtagacgg cgcgtctcga agcgacggcg cttacaggaa aaagcggtcg
     1441 catgagctgt cgcggtgatg tcgcatctac cgcggtcccc aaagtgggat taccgtgcgc
     1501 cggcgtgatg gattcgcgca tcggagcctc atgaaccgca aggttggcgc cctcgtcgct
     1561 gctgttctcg ttgctgctct cgccatctgg ttctttgcat ttcgcgatcg cagcagctcg
     1621 aagccgcagc ccaaagccgg tcgcaacggc aagatcgagc ttccgaagtc cacgcccgcg
     1681 aagaccgacg acacgccggc tccgcagggc atggcgccgc gatggtcgct cgatgtcgat
     1741 ccagaaggtc cgctccgtct cgaaggccag gtcgtcgatg aagacggcca tggtgtcggc
     1801 ggcgcagagg tgtggctcgg ctcggtgccg ccgcgctcgg cgaagagcga ggacgatggc
     1861 acgttcgcgt tcgacaagct ggtcggccgc gagtacgggc tgaccgcaac ggcgggcgaa
     1921 cgcgtcggcg gtccggttca atacaagctg accgagagca gcgatccggt cgtgatccgg
     1981 ctgacgaaga gcgcgaaggt cgtcgtcacg gttctcgccg acgacagcaa accgatcgaa
     2041 ggcgccgacg ttcgcctcgc cgcgatgggc gaacgcactg cgaagaccgc agcggacggc
     2101 accgcgacgc tctcgccggt cgcgcctggc tgggtcgccg tgcaagcgac tgcaaatggc
     2161 tacgcgccag gcaacggatt cacgcaggtc ggttcggccg gcgctgtcgg cacgatcaaa
     2221 gtgacattgc acaagggcgt cccggtcacc ggccgcgtgg tcgacgaagc cggcgcaccg
     2281 atcgcgaaag cgcacatgtc gacggccggc atctgggacc tcccgagcgc gactggctgc
     2341 gcgacatgca atcaggtcgt caccgatacg aacggtgagt tcacgttccc cgcactcggc
     2401 gccggaacgc acgtgctggt cgcgaccgat ggcgagcacg cgcccgcacg ctccgcgcct
     2461 gtcaccgtcg ccgatcaacc ggtcaagaac gtcaagatca cgatgaaggc gggcggcacg
     2521 ctcgccggca ctgtcgtcga cgatcagcag aagccggtgc cgttcgctac cgtgcgtgtc
     2581 ggcggcgacg gccagcagat gtggatggtc gacgcgcgcc aggcaacgag cgatcgcacc
     2641 ggccacttcg agctcaaagg cctcgcacgc gcaaagctca aggcgcgcgc cgagtccgac
     2701 atcgcggcga gcgcgatcgc gaccctcgat ctgacgagca ccccgaaggc cgagctcaag
     2761 ctcgtgctcg atgtcaaagg cacgatcgcc ggcgtcgtcg tcaacgagac gaacgagccc
     2821 gtcgccgagg tccaggtcaa cgcgttcccc gatattttcg gcggcaactc gcccgaatcg
     2881 atctcgctcg ccggcttctc gtcggccacc accgacggtg ccggccactt cgtgatccgc
     2941 ggcttgcccg acggcgggta tcgcctgcgc gcggcgcgtt cgaccggcgg tcgttatgac
     3001 tggggccagc aaggcacgcc cgcgaaggtc ggcgacaaga acgtgaagat cacgctcgcg
     3061 gccgagggca gcattgtcgg caagctcgtc gtcgaaggcg gcggtgcccc gaagctggcg
     3121 accgtgcagg tcggctccca accgagcacg ccggcaagcg ccgacggcac gttcaagctc
     3181 gaagacgtcg cgcccggcaa gtacgacctc cacgtgcgtg gccacgactt cgccgaattc
     3241 atccagcacg acgtcgaggt gaagcccggc aagccgaccg atgtcggcac gctcacgctg
     3301 atgcgcggcc gcaagctcgt cggcaaggtg atcgacgccg gtggcaatcc ggtccccggc
     3361 acgaaagtga agaccggcga catgctgttc tcgatgcagg gtggcgacga tcagatggag
     3421 aacctcgagg agatgtacgg cgcgcgcacc gacatcaccg atcaggacgg caagttcacg
     3481 ctgatcggca tcgcgaagaa atcgacgagc gtgatcgccg atcacccgac gcgcggccgt
     3541 tcggttgccg tctcgctgcc cgcgggcacc gacgatccgc cgcccgtgac gctgacgctc
     3601 aaaggctacg gctcgatcac cggcaaggtc acctcgaagg gcgagcccgt cggcggcgcg
     3661 acgatcaccg acacgcccaa aggtggtggc gcgcagattc gcatcgtgca aagcgaggcg
     3721 gacggcacgt tcacgctgac caagatcggc gaaggcacgc acgtggtcag cgcgatgcag
     3781 cagagcatgc tcggcatgtc actcaagtcg acgagcacga cggtgcaggt gcgcgagggc
     3841 aaagaatcga aggtggcgat cgacattccg gtcggctcgc tcgtgctcgc cgtgcaggtc
     3901 aaagcgcaag ctggccagaa ggtcgactcc gcgcagatct ttctgttccg cggcgccatc
     3961 gtcgtccaca acgcgaagga gctcaccgag ggcttcctta gcggcggcgt gcagggcatg
     4021 aagttctggc tcggcgaggg caagcccgtt cccgagttcg acgagctcgt cgccggtgac
     4081 tactcggtct gcacgattcc gatcaccggc gacctcaacg actcgcaatt ccaggcccgg
     4141 atccaggagc acatggacgt gctcaaggtc tactgcaaga aggtgccgct cgcggcgtca
     4201 ccgcagaagc agacgttcat cgccgaagtg ccggcgatga cgccgctgcc gcagaactaa
     4261 atttagtagc cgctgtcgta gtacgggttc gcgtagtagc cggagctgta cgtgttgctg
     4321 tagtagccag ccgcgttcgg atccggctcg tagtggcctg gcgtccagat ccactcgtag
     4381 ccatcccagt tccactgccc cgcaacccag tagtagcccg gcaccgccgc gtagttttcg
     4441 acgagcagcg ccggccgctg gtagtagttg tagtagtggt acgcgatgcg cggccgctgc
     4501 acgtagatcg gccgtcgcac atagcgatac gacggccgcg ccacgtagcc gcggttgtac
     4561 gtcacgtggc cgcgcggctg cacgtagcgg ccacgcgagt gcgtgacaga aacaccgccc
     4621 tgcgagtggc gtacgccgcg gcctcgatca gccgacgcaa cgccgccgaa ggcggtcaga
     4681 gcaacaagcg ccgagagaat gattcgcttc atgactacga tgtacgtacg gatcgccgtc
     4741 gacgctcgtt gcgttctgtt aaccagctac gagctactcc tagtagcgat cgtgatcgca
     4801 attggcgtcg tagccattgt cgtagctgga ataatcgtag ttcggctcga cgtaattcgg
     4861 atctggctcg tagtgacccg ccgtccacgt ccactcgtag ccggtccagc cccactgccc
     4921 cgagacccag tagtagccgg tcatcggcgc gtagttctcg gcgagcaccg ccggccgttg
     4981 gtagtaattg tagtagcggt agcggatccg cggcgccgtt acgaagatcg ggcggcgcac
     5041 cacgtgcgtg cgctggacgt aaacgcggcg tggctgctgc acgaccacgc gaggctgcac
     5101 gacgacgcgc ggcgcggtca cgcgtacgcc gccgctccag ttgttgccgc gttgccagcg
     5161 accgccgtgg tgacgatcag ccgacgcgac accgccgatg acagcgaggg tcaagatcga
     5221 aaggacaaga gttcttttca tggtggctcg atacgcagca ccatgtatgc ccgatcacgc
     5281 gcgcacgaat tttcggtgag ttgccacgta cggtgtgaac acgaggccac gtgcagcgtg
     5341 agccaaccgc tcggtgttct tcatacttcg caacaaagcg acgatcacca acaggcgatc
     5401 accaccacca gtagtagtca ttcggcgtgt agcccggcaa cgtttgctcg gcggtcttgc
     5461 cgagatcgct cacgcgatgc gcggtgaccg ctttgtcact gatcgcgtac acgaagtcgc
     5521 ccatgaagat gctgcggcga atgtccgtgt accgccagta cgtgttgccc tcggcgtcgt
     5581 agtacgggct gtgatcgatg cggcccttga tcgagagcgc gccgctcgtc ggatcgacat
     5641 tcacgagcac gagctgcgac aggtagcggt agtagtactg gccgttgccg gtgttctggt
     5701 acgcgtaggt cgactgcgga atcgcgagca ggcccttcgg tgcccagtac tggaacgcct
     5761 tgtggtcgta cagcgcttcc gaccaccccc aggtgttctg ggcttcgacc ggtagcgcgg
     5821 cgtcgagggc cggatgcgcg aagtcgctga cgtcgaacag cgagacctgc gtgcgccact
     5881 ggttcgtgcc gtcgacaccg atcgagagca gcttgccgtc tgcgatcgga tgcaggtacg
     5941 tcgagaagcc cggcaccttg agctcgccga ccacatgcgg gttgcggcga tccgacagat
     6001 cgagcgtgaa cagcggatcg acgtactggt acgtgacgag gtacgcctta ttgccctgga
     6061 agcgcgacga catgatgcgc tcgcccttcg cgatgccgcc gacgtgcccg acgatatcga
     6121 gcttgctgcc gtgatcctcg agcacccaga cgtggttctc catcgtcggc cgctcttcgt
     6181 catccgccca ccagcgccac aacaggccgg tcgtcgtcgc gacgcgaatc gcgccgttgt
     6241 cctcgtcgag cgagaactgg tcgacgatct ggccatcgac gcggccggag cccgtgtaca
     6301 cgctcttgcc cggcgtcgag atatcgaacg cgtggacgtt cagctgatcc ggatcgtcct
     6361 ggtaccacca gtaccaccac cagtcgtgcg ccggctcggc gagcagcagc gtgtccttcg
     6421 acgcgtagaa cgtcgaccag ttcgacacga tgtggtcggc atcccagtgc acggtgtcgc
     6481 cgagcagatc gaacgagatg atcgacgaga tgccgcgcgc gtggctgtcg gtcggccgat
     6541 agaacgagcg gcacgagccc tcggacaggc tgttcgacgt gaactgaccg ttcgggttgc
     6601 gcacgtagat ctgcgggatg aagtccgcga gcgcgaggct gttgacgtaa ttgcgcaccg
     6661 cgatcttcgt cgcggtcttg tcctcgccgt tctgctcgta caggttccac cagccccaca
     6721 tgatcgacgg ctcgatcatc gagtagccgg cgatgcggat gctcgaatcg atcttgcgcg
     6781 cggtctggta ccagccttcg taatagacct cgcgcacgag cgtcggcgcc gccttgttcg
     6841 tgatgtcgag gaccgtgatc ttcgacagct ggttgatgcg ccaatgccag ccgctctggt
     6901 catcttgata gccaacgagc tgcttgagcg gatggccgtc gggaagcgag tacgtgtcga
     6961 tccacgacag cacgaccact cggttcgcct tgctgtcgag cagcatctgc tgcggataac
     7021 cctcgatctt cgtgacgctc tccgcggtga gctcgccgaa gttcggcacg cccatgatgt
     7081 ggaggcgatt cccgttgagc gtgtagatgt gatagccgtc cgtcttcacg atgtcggcct
     7141 cgtcgacgcc ctgctcctgg ttgttggtgc ccgagaagtc gacgccttcc tggcgtccac
     7201 caccgctgcc gctaccggcg ccacccgctg cgtcgccctc cgcgccgccc tggccccagc
     7261 cccagtaatt cgcgcgatcg atatcggccc agatctcgta gatgaccatg ttcttgagat
     7321 cggtctcgag agccgagcag ctctggtact gcacgagctg cgtcgttttt gcgatcggat
     7381 gctcgccggg accatcgagg tcgtccgagc acgccgaaag ggccaacagc gatagggaaa
     7441 cgcaggccgc ggtgaagcgc atgcagcccc gttcagcaaa atcgcggcca gcctaactgt
     7501 gcgagacgtc atcccgcgat cgtcagatga ctcgcgtgtg ggatgatgac tgcctcagga
     7561 atctgagatg atccgtacgt gagactcctc gcgttcgttc tcgcggcggc agcagggtgc
     7621 ggtagctcga cgctctccgg cccgtgcagc ctcgactcgg cctgcagcga cggcgaggtt
     7681 tgcgacttca ccgccgaagg tggcccgatc tgcatctccg ccgacggtga cgtcgacaac
     7741 gacggcatcc cgaacggcga agacttctgc gagcacgcga tgggtggccg ctacgacgag
     7801 gacaaggacg gcgtcggcga cgattgcgac aagtgtccga tcgcaccgcc gcgcgacacg
     7861 ccagactccg atggcgacgg cgtcgagagc ccgtgcgatc cagcgccgac cgagccgggc
     7921 gacgagatca tcttcttcga cggtttcaac ggcgcgctct ccgccgactg gaaggcgatg
     7981 acgccagccg cctggaccgg aggtgatggc gaagtcaccg caaacctctc ggcggtcgcc
     8041 accgaggaca gcctctcgcg cgtcgtgcct ggcaagacga acgtcgcggt cgagatcggc
     8101 tacaaggtcg accgcgtcga aaacagctcg ccgcgccaca tggtcgcggt ctatgccgac
     8161 gatccacgcc ccgcaggtac cgcgaccgtt cagtgcggcg tgtcgcgcgc cgactcggcc
     8221 gctggcgacc tcgtgttcgt gcagacgaac tcgggccaga tgtcgtcgat gaccgtcgac
     8281 ccggcgctgc tcaccaccaa gagctatcgc gcgagcgcct atatgtcggg cgcgcgtgcc
     8341 ggctgcgtgg tgatcgcgaa caacatggcg ctcggaaccg tgcagacgac gatcacgccc
     8401 gacgatctat cgacgatcgc gctgaacgca cagtcggtga cggcgcgctt ccagtacgtc
     8461 ctcgtcgtcg gccactagga gtgcgtaggg catagcgtcg acggccccta ggagtgcgta
     8521 gggcatagcc tcgacgaagc acgcgtctgc ggccgcgccg cttccggagg aacgccaaga
     8581 cgccaaaacg ccaagaacgc caagaagcgt ttcgggatcg cgttctgctc gcgcgaccgc
     8641 gtcagcagat cgcgtcgaag atcgaccttc tggtggcgtc cttggcggct tggcttcttg
     8701 gcgtttagat ccggaacccg tcgtgcggcg ccgaaggcgc catcgcgctc cgccgacggc
     8761 ggtccactgc gcccggatcg cgcgtttcgc actcgaggtc tagcgccggt atttcagcag
     8821 cgcgagcgtc gagtcgacga tgtggcgctc ggcatccttc gagaggcaat acacctcttc
     8881 gtagtgatcg ccttcggcct cgttgagcca cgggcccgtc gtcgcgagca cgaagttgta
     8941 cgccggcacg atgtagccga cgtagtcctc ggcgagaccc gcgacaaccg gataccgcac
     9001 gccgtcgtgc gcgagcacga ggtcgcgcat gtacggcggc tgcggcgctt catcgaactt
     9061 cggcagattc ggcgtcggga cgcattcgac acccgtgttg cacatctgct gctcgttgca
     9121 gtcgttgttg ttgtcggcgc agtgacccgc atgctggagc agcggccagc cgaagctcca
     9181 ctcgccgttg tagccgccaa cccaaagctc gggatgaagc tcgcccggcg tggtcacgag
     9241 accgagcgga ccgatctgca cgtacgtcgc gcgcagcgga agccacggcg tgttcgtttc
     9301 gtcgatcggc tcgtcggggt tgtagccggc agtcggatgc gggccgagga tgccgaggat
     9361 gaagtagacc tggatgccaa ggttgtcgac gcgcgcgtag aacttcgcgc tcttgaagct
     9421 gagtggtagg tcgctcgtcg attcgccctt gtcgcgcagc gcggtcagcg cgaccgccgc
     9481 cgcattcgtg ccgagtgcct gatccatcgc gtgcgacgtc ttcgtgaccg gtacgccgcc
     9541 gggaccgggc ggatgcgtgc cgcgcagcga gccgatctgg ccaccgagtg cgccttgcac
     9601 gaacacggtg atgcccccga ggccggcgag atcctgcagt gcgtacttgc tctcggtcgt
     9661 cagcacgccg ttctcgacgt gatcgcgcag ccagtgcgga tagtgcgcgg tgatcgtcgc
     9721 cgggacggtg tcgtcgaagt gggcgacctc gggatggtcg gcccagttta cgagcgtgcc
     9781 gatcgtctcg ctcgggttac tgactttcac gaagcgcgcg atcgtcagcg tcggatccat
     9841 gatctgcggg tcgcgcaggt ctttccacca gtcgtccgtc ttgctctgct cgttcgtcgg
     9901 atcgttgatc gtcttgctcg acgcgatgat catctgcgcg ggttgcacgt tgttgaccgc
     9961 ttcggcgatc gcttcgactg cggccgcgtg cagcttgtcg atcacgaact gctcgcgacc
    10021 gctttcgccg acgcgcgggc cccagatgcc ggcagtgtcg ggcgcgtcgt gcgcgtgcgt
    10081 cgagccgatg atgatgtgat cgatgccgag gctcgcgagc gccggatcct cgcggatcga
    10141 gtccatgtcg ccgacgaaca tgccgacgca atcgacgtag gcgatcacga tgcgcatgtc
    10201 gccttcttcg aacgccatcg cgcgcacttc gacgtcggtc gtgacgccga gcgccgcgcg
    10261 cgcgccgccg aacagccaca cgccgtcgaa cttgccgttg ccgttgagat ccgtgtacgg
    10321 ctcggtcgtc tgccactgac gatccatgtt ctcgtcggtg tacgtctcga agttctgcgg
    10381 cgtgtacgtc cgcttcgcga cgccgacctt gagcacgccg tcgcccgcgg tcttgcactg
    10441 cggatcgccg ggacacacga ggtccggctt gtcgaaccct tcgagccgcg cgtcggcctc
    10501 gactttgtga ccgtcatcgc cgcagcccgc aagcacggcg attgcgaggg tcacccatcg
    10561 catcctcatc atggggcggt cgtacctcga cgagccgggc cgtgtaaatc gcgaagtatc
    10621 agggcgtgac gagatccgcg atttcgtcgt cggtgagcac gccggagtag acccggatgt
    10681 cgtcggcgac gccgatcagc tcgccgccga ggatgatctg accattctcg ctcaggatcg
    10741 aggcagccat cgtgccggct tccacgccgt cgagaacgat gcgtttcatc ttgccgtcgt
    10801 aactgatcgc gatgtgatgc cactcgccgg cggtcagcgt cgcgctcgtc gcttcgaacg
    10861 agctgaactc cgggtgcatg tcgggcgtca cgcagatcga ccacatcgcg cctttgatga
    10921 tcgggcagcc gggcagctgc ggtggcggca tgtcgacgaa cacccacgct gatgccgaga
    10981 agccacgtgc gacgtagtcg aaggcggcga ggctcggcac gtcgacctgc tggtcgccac
    11041 cgttgaagcg caacgcgcca ccgactttgc cggcctcggc cgtcgggcag accgtgcaca
    11101 tgccatcgta gtggttgccg ctggagtccg cgacggtgcc gccggtgccg ctgtccatcg
    11161 tgtaatgggc gacgagctgc gccgagtagt ccggcgggcc accggtgtca caggctacga
    11221 gaacgaccaa gaggagtgcg cgcactcctc atcgtaacgc gttatttcgc gccgaggcca
    11281 atcggacagc tcacaccggt gccgccgagg ccgcagtagc cgcccgggtt cttgcccagg
    11341 tactgctggt ggtagtcctc ggcgtaatag aacggcgggg cttcgcggat ctccgtggag
    11401 atgtcgccgt gaccggccgt gcgcaggcgg tcttgataca tggccttcga cgcctcggcc
    11461 gccttctgct gctcggcaga gaacgtgtag atgccgctgc gatactgcgt gccgacatcg
    11521 ttgccctggc gcatgctctg cgttggatcg tggctctccc agaacacgcc gagcagcttg
    11581 tcgtagctga cctgcttggg gtcatagatg acgagcacga cttcgttgtg gccggtcatg
    11641 ccgctgcaga cttcctcgta cgtgggattc ggcgtgaagc caccggcgta gccgacggcg
    11701 gtcgaataga cgcccggcgt ctcccagaac ttccgttccg cgccccagaa gcagcccatg
    11761 ccgaacagcg cacgctgcgt gccgtcggga aacggcggcg tgatccgatg accgttgacg
    11821 aagtgggtgt ccccgacctg catcgctgtc gaacggccgc gcagtgcctc gtcgggggac
    11881 ggcatgcggg acttcttggt gctgaagaac atgacaacca agtatacgac ctaggtcacg
    11941 gttggttact gtcgcctcat gaccggtgtg tctctcctcg ggatttgcgg cagcacgaga
    12001 aaagcgtcga tgaacgccgt gctgctgcgg accgtggctg aggcgctccc gcacgacgcc
    12061 aagctcgaga tctgggaagg cctcgatcac ttgccgatct tcaacagcga cctgcccgaa
    12121 ccgccgggca tcctcgcact caagggccgc atcgctgccg ccgacggcgt gatcttctcg
    12181 gtccccgagt acaactactc gatcccgggc gggctcaaga acgcgctcga ttgggtgtcg
    12241 cgcccgccgg cgatgtcacc gatgcgcggc aagccgatcg gccttgtcgg cgcggcgacc
    12301 ggcatgagcg gcacgatccg cgcgcagaat cacatgcgcc agatcctcgt ctacagcgac
    12361 tcacccgtga tgagccagcc cgaggtgctc atcccgcgcg cgcacgagcg tttcatcgac
    12421 ggcaagctca ccgacgagcc gacgcaccag ctcctccagc ggttcgcgac cgcgttcgtc
    12481 gcgttcgtgc accgccacaa ggttctcccc ggcgcttaga tgaacccctg cggaatctcc
    12541 cgcagtgggt cgtgtagccg cgcggcgcgt agcgacgccg cgagcgaagc gagcgcgccc
    12601 cgaaggggcg gcggctacac gaacgagtcg tgatacgccg cgtcctcgac ggacaccgcg
    12661 gccgacgtcg tgtgcagcgg caggaacgta tcgatcatca ccgcgagctc ggtcgtctct
    12721 ttcgtgccga tcgagcgctc gtacgcgccg ggatgcggac cgtgcggcac accgatcgga
    12781 tgatgcgaga tgctgccggg cccgacgccc ttgcgcgacg tgaagttgcc gtcggcgtag
    12841 aacaggatct cgtcgcagtg cgtggagctg tgcggatacg ggcacggaat cgcttcggga
    12901 tggaagtcga ccggccgcgg cacgaagctg cagatgagcg caccgcgcgc cgcgaacgtg
    12961 ccgtgccacg tcggcggcaa gtgcacgctc gacacgcgcg gttggaacgc gaggatcgga
    13021 aacgcccacg gatagacggc gccatcccag ccgacaacgt cgaacggcga gtgctcgagc
    13081 gtgaagccgt gccacttgcc gtcgcggcgc acgaccatgc cgcggatctt ctcgtcgtac
    13141 ggcggctgca gcttcgggcg cttgaagtcg cgctcgctgt acggcgcgtc catgcggagc
    13201 tggccgacct cgttgcgcca ctgacgcggg aggtgaaggc caccattgag cgagaaccag
    13261 aaccagtgct gctcgccctt cgggaggaac cgatgcaaga ggccgcgtgg cacgaacacg
    13321 taatcgccct gcgcgaattc gatatcgccg agcagcgagc gaagggtgcc gccacctttg
    13381 tggatgtaga cgaggtcgtc cgcatcgccg tt
//
LOCUS       DASUFX010000385         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_756684,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000385 DASUFX010000000
VERSION     DASUFX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756684"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2723
                     /gene="rpoC"
                     /locus_tag="VFV99_15465"
     CDS             <1..>2723
                     /gene="rpoC"
                     /locus_tag="VFV99_15465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5060766.1"
                     /translation="FRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKLALELFKPFI
                     FSKLQLRGEATTIKAAKRLVEREGPEVWDILEQVIREHPVMLNRAPTLHRLGIQAFEP
                     VLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLSIEAQLEARALMMSSNNILSPANGE
                     PIIVPSQDVVLGIYYMTRERANARGEGMLFSDVEEVHRAYESGVVELHSKIRVRIREK
                     VGGVPTSKLTNTTVGRSLLSEILPAGMPFEQINRAMNKRAISATINLCYRQLGLKETV
                     VFADQLMYTGFYYATRAGVSFGVNDMVVPKEKQAILDGAENEVKDIQDQYAAGLVTDG
                     ERYNKVVDIWSRTNDQVAKAMMEKLGVEPVTEGPGKGGQQESFNSIYMMADSGARGSA
                     AQIRQLAGMRGLMAKPDGSIIETPITANFREGLDVLQYFISTHGARKGLADTALKTAN
                     SGYLTRRLVDVAQDLVVTELDCGTTVGLLMTPLVEGGDIVEPLRERVLGRAAAVDVLA
                     PGSEKVVLKAGTLLDESAVKMLEQYSIDQVLVRSPITCETKYGVCAQCYGRDLARGHR
                     VNRGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRSAAVNSVEVKSAGTIRFVNVKTV
                     THEQKGHRITTSRSGELAVVDEFGRERERYKVPYGATLGAKDGEAVQPGQLVASWDPH
                     THPVVTEVSGYLKFEDFIDGVTVTSQVDEITGLTSIVVLDAAQRSSEYRPTAKLVDEK
                     GKAINFPNTDIPAAYALPSGAVLSMENGARVAVGDVLARLPQESSKTRDITGGLPRVA
                     DLFEARRPKDPAILAEYSGTVSFGKETKGKRRLVITDDEGEQHEELIPKWRHLNVFEG
                     ERVQRGEIIADGEPNPHDILRLQGVEPLANYLVREIQDVYRLQGVKINDKH"
BASE COUNT          498 a          890 c          905 g          430 t
ORIGIN      
        1 ttccgtcaga acctgctcgg caagcgcgtc gactactcgg gccgctcggt cattgtcgtt
       61 ggccccactt tgaagctgca tcagtgcggc ttgccgaaga agctcgcgct cgagctcttc
      121 aagcccttca ttttctcgaa gctgcagctg cgcggcgaag cgacgacgat caaagctgcc
      181 aagcgcttgg tcgagcgcga gggtcccgag gtttgggaca tcctcgagca ggtcatccgc
      241 gagcatccgg tcatgctgaa ccgcgcgccg acgctgcacc ggttgggcat tcaggcgttc
      301 gagcccgtcc tcatcgaggg caaggcgatc cagctgcacc cgcttgtctg cacggcgttc
      361 aacgccgact tcgacggcga ccaaatggcc gtccacgtgc cgctgtcgat cgaggctcag
      421 ctcgaggcgc gcgcgctcat gatgtcgtcc aacaacatcc tgtcgccggc gaacggcgag
      481 ccgatcatcg tgccgtcgca ggacgtcgtg ctcggcatct actacatgac gcgcgagcgc
      541 gcgaatgcgc gcggcgaagg catgctgttc tcggacgtcg aggaagtgca tcgcgcgtac
      601 gagtccggtg tcgtcgagct gcactcgaag atccgcgtgc gcattcgcga gaaggtcggc
      661 ggtgtgccca cctcgaagct cacgaacacg acggtgggcc gctcgttgct gtcggagatc
      721 ttgccggccg gtatgccgtt cgagcagatc aaccgcgcga tgaacaagcg cgcgatctcg
      781 gccacgatca acctctgcta tcgccagctc ggcctcaagg aaacggtcgt cttcgccgac
      841 cagctcatgt acacgggctt ctattacgcg acgcgtgccg gcgtctcgtt cggcgtcaac
      901 gacatggtcg tgccgaagga gaagcaggcg atcctcgacg gcgccgagaa cgaggtcaag
      961 gacatccagg accagtacgc ggcgggcctc gtcaccgacg gcgagcgtta caacaaggtc
     1021 gtcgacattt ggtcgcgcac gaacgaccag gtcgcgaaag cgatgatgga gaagctcggc
     1081 gtcgagccgg tgaccgaagg tcccggcaag ggcggccagc aagagtcgtt caactcgatc
     1141 tacatgatgg ccgactccgg cgcgcgcggt agcgccgcgc agattcgcca gttggccggc
     1201 atgcgcggcc tcatggcgaa gcccgacggc tcgatcatcg agaccccgat cacggcgaac
     1261 ttccgtgaag gcctcgacgt gttgcagtac ttcatctcga cgcacggcgc tcgtaagggt
     1321 ctcgccgaca cggcgctcaa gaccgcgaac tcgggctacc tgacgcggcg tctcgtcgac
     1381 gtcgcccagg atctcgtcgt cacggagctc gactgcggca ctacggtcgg gctattgatg
     1441 actccgctcg tcgaaggcgg cgacatcgtc gagccgctgc gcgagcgcgt gcttggccgc
     1501 gcggccgccg tcgatgtgct ggccccgggc tccgaaaagg tcgtgctcaa ggcgggcacg
     1561 ttgctcgacg agagcgccgt caagatgctc gagcagtact cgatcgatca ggtgctcgtg
     1621 cgctcgccga ttacctgcga gacgaagtac ggcgtttgcg cccagtgcta cggccgcgac
     1681 ttggcgcgcg gtcaccgcgt gaaccgcggc gaggccgtgg gcgtcatcgc ggcgcaatcg
     1741 atcggcgagc cgggcacgca gctcacgatg cgtacgttcc acatcggcgg cgccgcgtcg
     1801 cggtccgctg ccgtcaacag cgtcgaggtc aagagcgcgg gcacgattcg cttcgtcaac
     1861 gtgaagacgg tgacgcacga gcaaaaaggc caccgcatca cgacgtcgcg ctcgggcgag
     1921 ctcgccgttg tcgacgagtt cggccgcgag cgcgagcgct acaaggtgcc gtacggcgcg
     1981 acactcggtg cgaaggacgg cgaggccgtg cagccgggcc agctcgtcgc gagctgggat
     2041 ccgcacacgc accccgtcgt cacggaagtg tccggctacc tcaagttcga ggacttcatc
     2101 gacggcgtga ccgtgacgtc gcaggtcgac gagatcacgg gcttgaccag catcgtcgtg
     2161 ctcgatgccg cacagcgcag cagcgagtac cggccgacgg ccaagctcgt cgacgagaag
     2221 ggcaaggcga tcaacttccc gaacaccgac atcccggcgg cctacgcgct gccatcgggc
     2281 gccgtgctca gcatggaaaa cggcgctcgc gtggcggtcg gcgacgtgct cgcccgcttg
     2341 ccgcaggaga gctcgaagac gcgcgacatc acgggcggtc taccgcgcgt cgcggatttg
     2401 ttcgaggcgc gccgtccgaa ggacccggcg atcctcgccg agtactcggg cacggtcagc
     2461 ttcggcaagg agacgaaggg caagcgccgt ctcgtcatca cggacgacga gggcgagcag
     2521 cacgaggagc tcattccgaa gtggcggcac ctgaacgtct tcgagggcga gcgcgtgcag
     2581 cgcggcgaga tcatcgccga tggcgagccg aacccgcacg acatcctgcg tctgcaggga
     2641 gtcgagccgc tcgcgaacta cctcgtgcgc gagatccagg acgtctaccg cctgcagggc
     2701 gtcaagatca acgacaagca cat
//
LOCUS       DASUFX010000386         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_617345,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000386 DASUFX010000000
VERSION     DASUFX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617345"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VFV99_15470"
     CDS             complement(<1..1601)
                     /locus_tag="VFV99_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX5060767.1"
                     /translation="MSTAVAPRPTAALGGPAQRNAETGLAIAVVFVIALLILPLPAMM
                     LDLFLATSIGLSLVVLLTAMQTADALEFSSFPALLLLLTLFRLALNVSSTRLILTHGE
                     AGQVIHAFGQFVIGGNYAVGLVIFLILIGINFIVITKGAGRVAEVAARFTLDAMPGKQ
                     MAIDADLSAGLIDENEARRRRDEIARQADFYGAMDGSSKFVKGDAIAALLITGVNIIG
                     GMFVGVVQKGMSAGEAASHFTVLTVGEGLVAQIPALIVSTAAGIMVTRASGSSRMGAQ
                     LTAQLAAYPRAIWVSAAVLAAFGIVPGLPKIPFLVLGGTMALLARTAEKAESTRRSKA
                     HAEQVAAKEPEKVADPMRDLLQIDPIELEVGYAVIPLVDEKQGGDLLERIQLLRKQAA
                     LEMGILVPPIRVRDDIRLPANDYVIKLRGSEVARAEVMPRFMLALDTGSAIRAVEGIE
                     TTDPAFGLSARWIAATRRVEAESYGYVVVEPSTVIATHLMESLKANAAELLGRQDVQE
                     MVDTLKKSHPALVEEMIPAKISLGVL"
     gene            complement(1598..>2278)
                     /locus_tag="VFV99_15475"
     CDS             complement(1598..>2278)
                     /locus_tag="VFV99_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX5060768.1"
                     /translation="GIQSWAELVKSLFKLLIVTIAVRGAIVAAWPDTMALAEQGPAGF
                     LHVVKKYSVKLLMTAGLCYLALALFDYLYQVWQHERQLRMSREEIKQELRQSEGDPLV
                     KQRMRAMGRAFARRQMFRDVPKADVVITNPTHLAVALKYDPLVAPAPVVIAMGQRKVA
                     ERIKAIAKESGVPMIENKPIARALVAKAKVGQMIPSELYLVVAEILAFVIRRRILRGG
                     PGLREVLA"
BASE COUNT          406 a          744 c          753 g          375 t
ORIGIN      
        1 agcacgccga gcgagatctt cgccgggatc atctcctcga cgagtgccgg gtgcgacttc
       61 ttgagcgtgt ccaccatctc ctgcacgtcc tgcctgccta acaattcggc cgcattggcc
      121 ttgagcgact ccatgaggtg cgtcgcgatg accgttgacg gttcgacgac gacgtacccg
      181 tacgactcgg cctcgacgcg gcgggtagcc gcgatccagc gagcggacag cccgaacgcc
      241 gggtcggtag tctcgatgcc ctcgacggcg cggatggcgc ttccagtgtc gagcgccagc
      301 atgaaccgcg gcatgacctc ggcgcgcgcg acctcggagc cgcgcagctt gatgacgtag
      361 tcgttcgccg gaaggcgaat gtcgtcgcgc acgcgaatcg gtgggacgag aattcccatc
      421 tcgagcgccg cctgcttgcg gagcagttga atacgctcga gcagatcgcc accctgtttc
      481 tcgtcgacga gcggaatgac cgcgtaaccg acttcgagct cgattggatc gatctggagg
      541 aggtcgcgca ttggatcggc gaccttctcc ggctcctttg cggcgacctg ttcggcgtgc
      601 gccttcgagc ggcgtgtcga ctctgccttt tcggccgtgc gggccagcag cgccatcgtt
      661 ccgcctaaca cgaggaacgg gatcttcggg aggcccggga cgatgccgaa cgctgccagt
      721 acagctgcac tgacccagat tgcccgcggg tatgccgcga gctgcgcagt gagctgtgcg
      781 cccatgcgcg agctgcccga cgcgcgcgtg accatgatac cggccgccgt gctgacgatg
      841 agcgccggga tctgtgcgac gagtccttca ccgaccgtga gcaccgtgaa gtgcgacgcc
      901 gcctctccgg cggacatccc cttctgcacg acgccgacga acatgccgcc gatgatgttg
      961 acaccggtga tcagcagcgc cgcaatggcg tcacccttta cgaacttcga cgacccatcc
     1021 atcgcgccgt agaagtcggc ctgacgagcg atctcgtcac ggcgccgacg cgcttcgttc
     1081 tcatcgatca ggccagcgga cagatcggcg tcgatcgcca tctgcttgcc gggcatcgcg
     1141 tcgagcgtga agcgcgccgc aacctcggcg acgcggccgg cacccttcgt gatgacgatg
     1201 aagttgatgc cgatgaggat caggaagatc acgagaccga cggcgtagtt gccgccgatc
     1261 acgaactgac cgaatgcgtg aatgacctga cccgcctcgc catgcgtgag gatcagccgc
     1321 gtgctgctca cgttgagcgc aagccggaac agggtgagca gaagaagcag agccgggaac
     1381 gagctgaact cgagtgcgtc cgccgtctgc atcgcggtga gcagcaccac gagggaaagc
     1441 ccaatgctcg tcgcgaggaa caggtcgagc atcatcgcgg gcagcgggag aatcagcagc
     1501 gcgatgacga agacgaccgc gatcgcgagg cccgtctcgg cattgcgctg tgccgggcct
     1561 ccgagagcgg cggtggggcg cggagcgacg gcagtgctca tgcgagcacc tcacgcaggc
     1621 ccggcccgcc acgcagaatg cgccgacgaa tcacgaacgc gaggatctcg gcaaccacga
     1681 gatagagctc gctcggaatc atctgaccga ccttcgcctt cgcgacgagc gcgcgcgcga
     1741 tcggcttgtt ttcgatcatc ggtacgccgg actccttcgc gatcgccttg atgcgctcgg
     1801 cgaccttgcg ctggcccatc gcgatgacga cgggcgcagg cgcgaccagc ggatcgtact
     1861 tgagcgcgac ggcaaggtgg gtcgggttcg tgatgacgac gtccgccttc gggacgtcgc
     1921 ggaacatctg ccggcgcgcg aatgcgcggc ccatcgcgcg catgcgctgc ttgacgagcg
     1981 ggtcgccttc cgactgtcgc agctcctgct tgatctcctc gcgactcatg cgcagctgcc
     2041 gctcgtgctg ccagacctgg tacaggtagt cgaacagcgc gagtgcgaga tagcagagcc
     2101 ccgcggtcat gagcagcttg accgagtact tcttcacgac gtgcaggaag ccggccgggc
     2161 cctgctcggc gagcgccatg gtgtcgggcc aggcggcgac gatcgcgccg cgcacggcga
     2221 tcgtgacgat cagcagcttg aagagcgact tgacgagctc ggcccaggac tgaatgcc
//
LOCUS       DASUFX010000387         9120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2008005, whole genome shotgun sequence.
ACCESSION   DASUFX010000387 DASUFX010000000
VERSION     DASUFX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008005"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFV99_15480"
     CDS             complement(<1..714)
                     /locus_tag="VFV99_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060769.1"
                     /translation="MRLIWPFLAVTSGIAHADTAWSEYGVATPMRPATQLAEAGCEIE
                     ITLKGAIAEVEQRQRLTNPGPDPLAATTDFVLPAGAQLVGLELKLGSGKAEAALAVPA
                     GYAVDRVSSPAVLGADLAQLEALQPLDGRPRFRLIVQPIAAEQDAQITTRWTRVTDIR
                     GGALHLVLPGHDGKPCKGSIHAQPGPGTSVARVRVAGQESSSRTFSLEGADLALDVDL
                     AFKRNEPLVWTQSESLGDGF"
     gene            772..2052
                     /locus_tag="VFV99_15485"
     CDS             772..2052
                     /locus_tag="VFV99_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5060770.1"
                     /translation="MRVVQAPPSRPKIGFVLSGGGSRGAYEAGIIHYIRTDLAKRLGR
                     HVPIDIVTGTSVGAINAAFLAATMTDPETQAQQVVAGWRGLQLEELISLKAGDLLRGF
                     RLLLGGDPPPPTPGSFRYGGLLDTSGLERFVIRIVPWRGIERSLRSKALHAISVSATH
                     VGTGHTVVFLSSGEPVPREWSRDPFVRHRAARIGPRHVLASAAIPLLFPAVKIGDEYF
                     TDGGLRQNTPMSPAIRLGADRLLLVSLRHVKKGEPPQIEKERVEAYPKPLFLAGKALN
                     ALMLDHTEYDLMRMQRINMILEAGRASFGDRFEEMMNHELVRLRGAPLRRIQAVHVRP
                     SEDIGAIAADFAASGKVKVRGPIAKRLISRLAAGEARHESDLLSYLLFDGDFAAELIE
                     LGRRDAAKKEDELAALFDVSGAAREPAAALPETA"
     gene            2104..5052
                     /locus_tag="VFV99_15490"
     CDS             2104..5052
                     /locus_tag="VFV99_15490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5060771.1"
                     /translation="MSKRLLTLALAALVACGGSPKTTPTTTGTTTATTPTSTPASSTD
                     PDAAPLPLWSKLKKGTLPNGLTYYILPHKKPEKRALLWLAVNAGSAQEDDDQRGLAHF
                     DEHMAFNGTKRFKENELVKYLESIGMRFGADLNARTSWDDTVYQLEVPSDKPEFMGKG
                     LDILRDWAGDVTYDPKEVDKERGVVLEEWRLGRGAGMRLFDKHAKVMFKGSRYANRIT
                     IGDPDTLKKAPVDKLVRYYKDWYRPNNMAVIAVGDFDPEAMEKEIVQRFQNLENPKNE
                     RKRPSGGVPEAKGTRVSIETDREATSTTVSIENPVAHRPETSKKDYRRILSEQIYTGI
                     LNERLASIARRGDAPFITAGGQIAGAIGVRDVDSFSRFAVAKKGNIEDALRALLTEVV
                     RIEKHGITSSELERARTNMTRFYESLADTEATRDSSDFTEEITRNFFEGELIVGSQTE
                     KKLALDILPTLTIAELNKIGTSLGGEDGRVILISGPEGKPLPTQERVLQIAKEVSSAK
                     VDAWEDAAPQTALMDKAPTAGTITKETKNDKVGVTEWTLSNGVRVIVKPTDFEADSVS
                     ISGSSPGGDAMAKDTDFPSTRFADDVADLGGVATFDVETLGKMLAGKQVSVSTGIGET
                     TESVSANGSPKDLETMMQLLYLRMTAPRKDVEAFNVWKTNFKEQIENALRSPEFRYAR
                     ESNTAEWKGNLRRKPPEPAEIGKIDQDKALAFYKQRFGDASDFTFVIVGSVKLETLKP
                     LVETYLASLPAKGRKEKEKDLKIRRVGGIVKKSFKLGTEPKASVSIEFHGPDKWTRDN
                     DRDIAILGQVLSVKLREMLREDMGGVYGVGAGGRISRSPYQERTFSIRFGCDPTRVDE
                     LVKASFDAAAKLAKDGADDATLASIKETFVRARETDLRTNRFWLGWLASAYKYGDDPA
                     LVLDTAPFVARIKSDLVKASAKRYLDAKQYYEAVMLPENADAAAKPAEAKADKPAKPG
                     EKPAKPAEKK"
     gene            5094..5789
                     /locus_tag="VFV99_15495"
     CDS             5094..5789
                     /locus_tag="VFV99_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060772.1"
                     /translation="MRRAIVVVVLLLGCREKPAPQKREPPPATSRNTTGAFAKPTRRL
                     PPTLATTFISSVAEASDSAAAWTAIADAYENELATCTKDCRAIAYEIVLARKNVVKAM
                     KLEPPPGDGPVELPAEVKEVVDAIDAYVKMVDPTDDEVAPMQFLAANALWHWRQPEAL
                     TRLQQFLEEHRDDPTAEYAANQLLHSLMQQNRLEELRAWVGELSGDETFLANKPELRA
                     TIARIQEALASNP"
     gene            6047..6487
                     /locus_tag="VFV99_15500"
     CDS             6047..6487
                     /locus_tag="VFV99_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060773.1"
                     /translation="MEVIARRELIEEIQLEPPPGYGPVELPHPVRSLLDTVDEYVKGL
                     QPDDTEIPAMRLLTARTLLHWRRLEALPLIEKFLRDYRDDENAEWAADSHMQQLINEQ
                     RFRELRIWLAAFSADEKFLANKPELRATLERAKFLLRARSDLLM"
     gene            6506..7192
                     /locus_tag="VFV99_15505"
     CDS             6506..7192
                     /locus_tag="VFV99_15505"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5060774.1"
                     /translation="MRLHLKQYTEPIFEVLAGYRATLVAHPLLTAARAGELSRETLHE
                     FAFHQYSDSILWIPMLAQMKAMARKSARLRKAIEDNIAHEAGLGNVSHVQLAVALMRS
                     LGITSLEPFPTRTFERSATLWLSDAFCDQSEPEVAGWLLVAESLVPLMFAALQPSFVA
                     LGADTRYFGEHVEVDTDEHAAWMAESVEDVLDIYGPAASEAITAGMVDAWQETREVPD
                     ALWRKQCASH"
     gene            7177..8121
                     /locus_tag="VFV99_15510"
     CDS             7177..8121
                     /locus_tag="VFV99_15510"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060775.1"
                     /translation="MRIALTYNEKRSASEAEAEFDSPETIHAIARMLASLGHSVTPVE
                     VSRPVEAIVAELRRLAPDLVFNMAEGQVGTFREAFYPALFEHLGLPHTGSPASVLALC
                     LDKAMAKKIVAAARVHTPRGQLVRRLDELRPVTCKVIVKPNFEGSSKGITQASVVDDP
                     ATLHDVVAGVLARYPAGVLVEEFVEGMDVSVGFVDGLGVLAPIGYAYAADGPHQIYDL
                     ALKQGPPERIDVHIPARLDELTTSRLVAAAKRAFAALGVTGYGRADFRVTPEGNVQFL
                     ELNPLPSLAPRERDLYAAAAPLGKAPRDLLACIVTAAA"
     gene            complement(8127..>9120)
                     /locus_tag="VFV99_15515"
     CDS             complement(8127..>9120)
                     /locus_tag="VFV99_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060776.1"
                     /translation="IPAGSVDIQISAPLTDPKPAQIVLEHCDDIGKFFELAKTQTPGA
                     CRIVMQGTSTLELDVWVENNTLRLGAHKGAVPAGKPGGMTAFGRELASGTWTAAFWGR
                     GTMLNLGTITAAKENAPPEVALGIHAMSLVNELGVAARVDKQGITFRAVLRTAWDNPP
                     AVVDKITKFPGNDILTGKATEPAKAIAAGAPGSPFAADFDAGQGGLMVPAAMIGLASA
                     VVIPIVMRATGAGGGEQDESAHGPQVNQSDLVMLLLHAYAEEAYPKWQADHPKQKCPA
                     TLAEVATYFGDNPGLPVEKDPWDHDLVMKCDDKGFSVSSVGPDGKAGTDDDVHL"
BASE COUNT         1646 a         2987 c         3075 g         1412 t
ORIGIN      
        1 gaaaccgtcg ccgagcgatt cgctttgcgt ccacacgagt ggctcgttgc gcttgaacgc
       61 gaggtcgacg tcgagcgcga ggtcggcgcc ttcgagcgag aacgtgcgcg acgacgattc
      121 ctgacccgcg acgcgcacgc gtgcgaccga ggtgccgggg ccgggctgcg cgtggatcga
      181 acccttgcat ggcttgccgt catgacccgg gagcacgagg tgcagtgcgc cgccgcgaat
      241 gtctgtcacg cgcgtccagc gcgtcgtgat ctgcgcgtct tgttcggcgg cgatcggctg
      301 cacgatgaga cggaaccgcg gtcggccatc gagtggctgc agcgcttcga gctgcgcgag
      361 gtccgcgccg agcacggccg gcgagctcac gcgatcgacc gcgtagccgg cgggcaccgc
      421 gagcgcggcc tcggccttgc cgctccccag cttgagctcg agaccgacga gctgtgctcc
      481 cgcgggcagg acgaagtcgg tcgtggcggc gagcgggtcg gggccgggat tcgtgagccg
      541 ctgccgctgc tcgacttcgg cgatcgcgcc cttcagcgtg atctcgatct cgcagcccgc
      601 ttcggcgagc tgggtcgccg ggcgcatcgg tgtggctacg ccgtactccg accacgcggt
      661 atctgcgtgc gcaataccgg atgtgacggc gaggaacggc cagatcaggc gcatgtgctt
      721 gagacaacga gggtaccgaa cggttgcgcg cacatcacgg tcgacgcgac aatgagggta
      781 gtgcaagctc cgccgagcag accaaagatc ggcttcgtgt tgtcgggagg cgggtctcga
      841 ggggcctacg aggccggcat cattcactac atacgcaccg acctggcaaa gcggctcggt
      901 cgccacgttc cgatcgacat cgtgaccggc accagcgtcg gggccatcaa tgcggcgttc
      961 cttgccgcca cgatgaccga tccggaaacc caggcgcaac aggtcgttgc ggggtggcgc
     1021 gggctgcagc tcgaggagct gatcagcctc aaggcgggcg acttgctccg cggcttccgc
     1081 ctcttgctcg gcggcgatcc gccaccgcca acccccggca gcttccgcta cggcggcctg
     1141 ctcgacacga gcgggctcga gcgattcgtc atccgcatcg tgccgtggcg cggcatcgaa
     1201 cgcagcctgc gctcgaaggc gctgcacgcg atctcggtgt cggcgacgca cgtcggcacc
     1261 ggccatacgg tcgtgttctt gtcgagcggc gagcccgtgc cccgggagtg gagccgcgat
     1321 ccgttcgtgc ggcatcgcgc ggcgcgcatc ggtccgcgtc acgtgctcgc gtcggctgcg
     1381 attccgctgc tgttccccgc ggtgaagatc ggcgacgagt acttcaccga cggcggccta
     1441 cggcagaaca cgccgatgtc accggcgatc cggctcggcg ccgatcggct cttgctcgtg
     1501 tcgttgcgtc acgtgaagaa gggcgagccg ccgcagatcg agaaggaacg cgtcgaggcc
     1561 tatccgaaac cgctgttcct cgccggcaag gcgctcaacg cgctgatgct cgaccacacc
     1621 gagtacgacc tgatgcgcat gcagcggatc aacatgatcc tcgaagccgg gcgcgcgtcg
     1681 ttcggcgatc gtttcgaaga gatgatgaat cacgagctcg tgcgcctgcg cggcgcgccg
     1741 ctccgccgca tccaagccgt ccacgttcgc ccgtcggaag atatcggtgc gatcgcggcc
     1801 gacttcgcgg cctcgggaaa agtgaaggtc cgcggtccga tcgcgaaacg gctgatcagt
     1861 cggctcgcgg cgggcgaagc gcgccacgaa agcgacttgc tctcgtatct tctgttcgac
     1921 ggcgacttcg ccgccgagct gatcgagctc ggacgacgcg acgcggccaa gaaagaagac
     1981 gagctcgccg cgctgttcga tgtctcgggt gcggcccgag agcccgccgc ggcacttccc
     2041 gagaccgcct gagacacggt gcgacgggtg cacctgcgcg attgacgtgg caatgtcccc
     2101 gccatgtcga agcgcctgct caccctcgct ctcgccgcgc ttgtcgcgtg cggtggttct
     2161 ccgaaaacca cgccgaccac gaccggcacc acgacggcaa cgacaccaac gtcgacgccg
     2221 gcgagcagta cggatcccga cgccgcacca ctgccgctgt ggagcaagct caagaagggc
     2281 acgctgccga acggcctcac gtactacatc cttccgcaca agaagccgga gaagcgcgcg
     2341 ctgctctggc tcgcggtcaa cgcgggctcg gcgcaagagg acgacgatca gcgcggcctc
     2401 gctcacttcg acgagcacat ggcgttcaac ggtacgaagc gcttcaagga gaacgagctc
     2461 gtcaaatacc tcgagagtat cggcatgcgc ttcggcgccg acctcaacgc gcgcacctcg
     2521 tgggacgaca ccgtctatca gctcgaggtg ccgagcgata aacccgagtt catgggcaag
     2581 ggcctcgaca tcctgcgcga ctgggccggc gacgtgacgt acgacccgaa agaggtcgac
     2641 aaggaacgcg gcgtcgtgct cgaggagtgg cgactcggcc gtggcgccgg catgcgcctg
     2701 ttcgacaagc acgcgaaggt gatgttcaag ggcagccgct acgcgaaccg catcacgatc
     2761 ggcgaccccg acacgctcaa gaaggcaccg gtcgacaagc tcgtccgcta ctacaaggac
     2821 tggtaccggc cgaacaacat ggcggtgatc gcggtcggcg atttcgatcc ggaggcgatg
     2881 gagaaagaga tcgtccagcg cttccagaat ctcgagaatc cgaagaacga gcgcaagcgc
     2941 ccgagcggcg gcgtgccgga agcgaagggc acgcgcgtct cgatcgagac cgaccgcgaa
     3001 gcgaccagca cgaccgtgtc gatcgagaac ccggtcgcgc atcgtcccga aacgagcaag
     3061 aaggactacc gccgcatcct cagcgagcag atctacacgg gcatcctcaa cgagcgcctg
     3121 gcgtcgatcg cacgtcgcgg cgacgcgccg ttcatcaccg cgggtggcca gatcgccggc
     3181 gcgatcggtg tgcgtgacgt cgacagcttc tcgcgcttcg cggtcgcgaa gaaggggaac
     3241 atcgaggatg cgctgcgcgc gctgctcacc gaggtcgtgc gcatcgagaa acacggcatc
     3301 acgtcgagcg agctcgaacg cgcgcgcacg aacatgacgc gcttctacga gtcgctcgcc
     3361 gacaccgagg cgacgcgcga cagcagcgac ttcaccgaag agatcacgcg caacttcttc
     3421 gagggtgagc tgatcgtcgg ctcgcagacc gagaagaagc tcgcgctcga catcctgccg
     3481 acgctgacca tcgccgagct caacaagatc ggcacctcgc tcggcggcga agacggccgc
     3541 gtgatcctga tctccggtcc cgagggcaaa ccgctaccga cgcaagagcg cgtgctccag
     3601 atcgcgaagg aagtcagcag cgcgaaggtc gacgcgtggg aggacgcggc gccgcagacc
     3661 gcgctcatgg ataaggcgcc gacggccggc acgatcacga aggagacgaa gaacgacaag
     3721 gtcggcgtca ccgagtggac gctgtccaac ggcgtgcgcg tgatcgtgaa gccaaccgac
     3781 ttcgaggccg acagcgtctc gatcagcggc agctcgccgg gcggtgacgc gatggcgaag
     3841 gacacagact tcccgagcac gcggtttgcg gacgacgtcg ccgacctcgg cggtgtcgcc
     3901 acgttcgacg tcgagacgct cggcaagatg ctcgccggca agcaggtgag cgtatcgacc
     3961 ggcatcggcg agacgaccga gtccgtcagc gcgaacggct cgccgaaaga cctcgagacg
     4021 atgatgcagc tgctctacct gcggatgacg gcgccgcgca aagacgtcga ggcgttcaac
     4081 gtctggaaga cgaatttcaa ggaacagatc gagaacgcac tgcgctcgcc cgaattccgt
     4141 tatgcgcgcg aatcgaacac cgcggagtgg aagggcaacc tgcgccgcaa gccgcccgag
     4201 cccgccgaga tcggcaagat cgatcaggac aaggcgctcg ccttctacaa gcagcggttc
     4261 ggcgacgcgt ccgacttcac gttcgtgatc gtcggctcgg tcaagctcga gacgctcaag
     4321 ccgctcgtcg agacgtacct cgcaagcctg ccggcgaagg gccgcaagga gaaagagaag
     4381 gacctcaaga tccgccgcgt cggtggcatc gtgaagaagt cgttcaagct cggtaccgag
     4441 ccgaaggcat ccgtctcgat cgagttccac ggcccggaca agtggacgcg cgataacgat
     4501 cgagacatcg cgatcctcgg ccaggtgctc tcggtcaagc tgcgcgagat gctgcgcgag
     4561 gacatgggcg gcgtttacgg cgtcggcgcc ggtggtcgca tctcgcgttc gccctaccag
     4621 gagcgcacgt tctcgatccg cttcggctgc gacccgacgc gcgtcgacga gctcgtcaag
     4681 gcatcgttcg acgcggccgc gaagctcgcg aaggacggcg ccgacgacgc cacgctcgcc
     4741 agcatcaagg agacgttcgt gcgcgcgcgc gagaccgacc tgcgcacgaa ccggttctgg
     4801 cttggctggc tcgccagcgc ttacaaatac ggtgacgatc cggcgctcgt gctcgacacc
     4861 gcgccgttcg tcgcgcgcat caagtccgac ctcgtgaagg cgtcggcgaa gcgctacctc
     4921 gatgcgaagc agtactacga agccgtgatg ctgcccgaga acgccgacgc ggcggcgaag
     4981 ccggccgagg cgaaggccga caagcccgcg aagccgggcg agaagcccgc aaagccggcc
     5041 gaaaagaagt agttgcggcc gcaactggcg gccggtcatg gtgtctgcgc tccatgcgcc
     5101 gcgccatcgt cgtagtggtt ctgttgctcg gctgccgcga gaagcccgcc ccgcagaagc
     5161 gcgaaccacc gcccgccacg tcgcggaaca cgacgggcgc gttcgcgaag ccgacgcggc
     5221 gactgccgcc gacgctggcg acgacattca tctcgtcggt cgccgaggcg agcgacagtg
     5281 ccgcagcatg gacggcgatc gcggacgctt acgagaacga gctcgcgacg tgcacgaaag
     5341 actgccgcgc gatcgcgtac gagatcgtgc tcgcgcgcaa gaacgttgtg aaggcgatga
     5401 agctcgagcc gccgcccggt gacgggccgg tggagctgcc ggccgaggtc aaggaagtgg
     5461 tcgacgcgat cgacgcgtac gtgaaaatgg tcgatccgac cgacgacgaa gtcgcgccga
     5521 tgcagttcct cgctgcgaat gcgctgtggc actggcgtca gcccgaggcg ctgacgcgac
     5581 tccagcagtt cctcgaagag caccgtgacg atccgacggc ggaatacgct gccaatcagc
     5641 tgctgcattc gctgatgcag caaaatcgcc tggaagagct gcgggcgtgg gttggtgagc
     5701 tttcaggtga cgaaacgttt ctcgcgaaca agccggagtt acgcgccacg atcgcgcgca
     5761 tccaagaagc gctcgcgtcg aatccgtagt cggcgcaact ggtgacagcg cgcgatgtct
     5821 gcggcacatg cgcagcgtca tcgccgtcgt tctactcgtc ggatgtctag aaacgccagc
     5881 tccgcagcag cgtgagccac tgcctgtcgc aagcggaaac caaacgccgc caaaacggcc
     5941 cacgcttgcg agcacgttca tcgacgcggg gtcgtggacg tatatcgcct acactgccaa
     6001 gagccggctc gcgagctgca cgacggactg caggtcgttc gggtacatgg aggtcatcgc
     6061 gcgcagggag ctgatcgagg agattcagct agagccgccg cccggctacg ggccggtcga
     6121 gctgccgcac ccggtgcgat cgctgctcga tacggtcgat gagtacgtga aagggctcca
     6181 gccggacgac accgagatcc cagcgatgcg gctcttgacc gcgaggacgc tcctgcactg
     6241 gcgccggctc gaagcgctgc cgctgatcga gaagtttctg cgggactacc gcgacgacga
     6301 gaacgccgag tgggccgcgg acagtcacat gcagcagctg atcaacgagc agcgtttccg
     6361 cgagctgcgc atctggctcg cggcgttttc ggcggacgag aagtttctcg cgaacaagcc
     6421 ggagctgcgc gcgacactcg agcgcgcaaa atttctcttg cgtgcgcgca gcgatctcct
     6481 aatgtgaatt ttgaaggagg tctgcatgcg tctacatctc aagcagtaca ccgaacccat
     6541 cttcgaagtg ctcgccggct accgtgccac gctggtcgct catccactgc tgaccgcggc
     6601 gcgagccgga gagctctcga gagagacatt gcacgagttc gcgtttcacc agtactcaga
     6661 cagcatcctg tggattccga tgctcgccca gatgaaggcg atggctcgca agtccgcccg
     6721 cttgcgcaaa gcgatcgagg acaacatcgc gcacgaggcg gggctcggta acgtctcgca
     6781 cgtgcagctc gcggtcgcgc tgatgcgcag cctcgggatc acgagcctag agccgtttcc
     6841 gacgcgcacg ttcgagcgct cggcgacgct gtggctgtcg gatgcgttct gcgatcagag
     6901 cgagcccgag gtcgccggct ggctgctcgt cgcggagtcg ctcgtgccgc tgatgttcgc
     6961 ggcgttgcag ccgagcttcg tcgcgcttgg cgccgacacg cggtacttcg gcgagcacgt
     7021 cgaggtcgat accgacgagc acgcggcctg gatggccgag tccgtcgagg acgtgctcga
     7081 catctatggt ccggcggcga gcgaggcaat caccgccggc atggtcgacg cctggcagga
     7141 gacgcgcgag gtccccgacg cgctgtggag gaagcaatgc gcatcgcact gacctacaac
     7201 gagaagcgtt ccgcgagcga agccgaagcg gagttcgact cgcccgagac gatccacgcg
     7261 atcgcgcgca tgctcgcgag cctcggccat agcgtgacgc cggtcgaagt gtcgcgaccg
     7321 gtcgaagcga tcgtcgccga gctgcgccgg ctggcgccgg atctcgtatt caacatggca
     7381 gaggggcagg tgggaacgtt tcgcgaagcg ttctatccgg cgctgttcga gcacctcggg
     7441 ttgccgcaca ccggcagccc ggcgagcgtg ctcgcgctct gcctcgacaa ggcgatggcg
     7501 aagaagatcg tcgcggctgc tcgcgtgcac acgccgcgcg gtcagctcgt gcgcaggctc
     7561 gacgagctgc gcccggtcac gtgcaaagtg atcgtgaagc cgaacttcga gggctcgagc
     7621 aagggcatca cgcaggcgag cgtggtcgat gatccggcaa cgctgcatga cgtcgtcgcc
     7681 ggcgtgctcg cgcgctatcc ggccggcgtg ctcgtcgagg aattcgtcga gggcatggac
     7741 gtgtcggtcg gctttgtcga cgggctcggt gtgctcgcgc cgatcggcta cgcgtacgcc
     7801 gcggacgggc cgcaccagat ctacgatctg gcgctcaagc aaggcccgcc ggagcggatc
     7861 gacgtccaca tccccgcgcg actcgacgag ctgacgactt cgcggctcgt cgcggcggca
     7921 aagcgtgcgt tcgcggcact cggggtgacg ggctatgggc gcgcggattt tcgcgtgacg
     7981 ccggaaggga acgtgcagtt cctcgagctc aatccgctgc cgtcactcgc gccgcgcgag
     8041 cgcgacttgt acgcggcggc cgcaccgctc ggcaaagcgc cgcgcgactt gctcgcgtgt
     8101 atcgtcacag cagccgcgta acgcgtctat agatgtacgt cgtcgtcggt gccggctttg
     8161 ccatccggcc cgactgacga cacgctgaag cccttgtcgt cgcacttcat cacgaggtcg
     8221 tggtcccacg gatctttttc gacgggcaag ccagggttgt cgccgaagta cgtcgcgacc
     8281 tcggcgagcg tcgccggaca cttctgcttc gggtgatcgg cttgccactt cgggtacgcc
     8341 tcttcggcgt acgcgtggag cagcagcatg acgaggtcgg actggttgac ctgcgggccg
     8401 tgcgccgatt catcctgctc gccaccgccg gcgcccgtcg cgcgcatcac gatgggaatc
     8461 acgaccgccg acgcgagacc gatcatcgca gcgggcacca tcaagccgcc ttggccggca
     8521 tcgaagtcgg cagcgaacgg cgagcccggc gcacctgccg cgatcgcctt cgccggttcg
     8581 gtggccttgc cggtgaggat gtcgttgccg ggaaacttcg tgatcttgtc gacgacggcc
     8641 ggcggattgt cccaggccgt gcgcagcacc gcgcggaacg tgatgccctg cttgtcgacg
     8701 cgcgccgcga cgccgagctc gttgacgagc gacatcgcgt gaatgcccag cgcgacctcg
     8761 ggtggcgcgt tttccttcgc agccgtgatc gtgccgaggt tgagcatcgt gccgcggccc
     8821 cagaatgcgg cggtccacgt gccgctcgcc agctcgcgcc cgaacgcggt cataccgccg
     8881 ggcttgcctg cgggcactgc gcctttgtgc gcgccgagac gaagcgtgtt gttctcgacc
     8941 cacacgtcga gctcgagcgt gctcgtgcct tgcatgacga tgcggcatgc gcccggcgtt
     9001 tgcgtcttcg cgagctcgaa gaacttgccg atatcgtcgc agtgctcgag cacgatctgc
     9061 gccggtttcg gatcggtgag cggcgccgag atctggatgt cgaccgagcc ggccgggatg
//
LOCUS       DASUFX010000388        16882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_480455,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000388 DASUFX010000000
VERSION     DASUFX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16882
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480455"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(801..6413)
                     /locus_tag="VFV99_15520"
     CDS             complement(801..6413)
                     /locus_tag="VFV99_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX5060777.1"
                     /translation="MSKRSDLLVVCLVAGTFGTANAKTDKKDTTAKTWVVDASHGTFS
                     EQIRVEVPAFRDLTPNLGLKYDSSDGNGWVGVGWSLEGIGIVERASPGKGAPKYNASD
                     IFLLDGEELVACVAGSVSPSCTSGGTHSTKVENYLRIALTGTGTASRWTVTQKDGTRR
                     IYAPVYTVNNNVDVYRWGLSQVIDTRGNTVNYTWAVNQFGCCWEYPQSVSYNGTSVTF
                     YYEQRTDRPSNAVGVGVRNVFGRIKTIDVTVSGSRVRAYKLSYGTSGSTSRSLLTSVQ
                     QFGTDATLDGTGTVTGGTSLPAITMQYQSGAPSFVSGTTDNGVSNNTDADYFAIDING
                     DGKTDMLEIYNACFILCTRYRVAHISTGSGFVQSSSDSGMATATTTRFLTADVNGDGK
                     GDIIELYESWGSERRRVWLSDGTRFNQASDACCMKTTSADSRYFALDVNGDGKTDLVE
                     QYPSVLFGAEGRRVWFSNGTAFTEGQTLNFDTDPKYLVLTLDVNGDGKMDMVQLKDYF
                     SAVQRTTWISTGTGFATGVTDQCDGQVVEAQKDGVVTAWSPFLAMDVNGDGKDDLVNL
                     ASFFGNRVRRVWHSTGKGFTEVSEVSVPFYDAGDWFLAEDVNGDGRSDLITIESVGLG
                     IEQRKIWLSVGDNFVGGAVDTQSAFGDGTKLLAADVNGDGLSEMVTMDSFFGIRSRRI
                     WNMTGPYPDLMTSIRGSLGATTSVAYAPSSAWTNTNNPPLMQTAASITLNDGRGGVWT
                     TNYTYSGGLYDRIEHRFMGFRYEKETRPCITGESVCPYNETWFRQDYGSASKPERIDH
                     RAGNGQLLSSEIYEYTTNGATVPWTSLRTGNWDYTFINAGTACPGADCKRKYVSRTFN
                     AYGDAVTEVEYGDYDTTGDEDTQSNTFVPNTTAYIVNKPADVKSFAGVGTAGTLLNET
                     LTYYDGAATWNQAPSVGNPTKESRWLSSPSSFVNTSKEYDTYGNVTATINALGARTTT
                     AFDATYHIFATSETNALSQVTTTSWNTVCGEKSQLVDLNSQTTALTYDPLCRLSQRTE
                     PGGNFERHTWLGLGDATSQYEQIETPAADGGATAMWRREYLDGHKRIWRTVKQGPAAS
                     GNIYVDTGYNARGEQRSKTRPYYWASGPQPTTYTTTTDYDALDRPIKVTHADGSFKTT
                     SYSLWTTTETDELGHSMINIKDADGRRVTHKEYVGGAYQVTAYLYDARGNLIRSTDPS
                     GNSINYVNDSLARTTAMTDPDSGASSYAFDGANRLTSQTDAKAQRTDFTYDALDRKTG
                     KTSKAGTGSAVTVSWTYDQVRTGYFNKGKLTAMTDSAGSKTLDYDAAGRSVKTVRVTG
                     GTSYTFTRGFDAGGRALWTTYPDGDTLGTPGTPLVYDSAGRLASIPGYVTSVLYGADD
                     KVARIDSANGAVNTRTYSAQRGWLTRISTTAGATTIQNLGYTRNARGLITSVTSPIAN
                     EGWTYAYDELDRLLSATNTSDATQNQTFTYNAIGNITANSRLGSYTYGTSRPHAVTAA
                     GSNTYSYDAAGLMTAGGGRTLTWDGDNRLATISGPANVAYTYDADGARVQQIENGITR
                     RFLGDNFEIQVGGNASKYVTVGDITVARRDGTTSYWVHTDHLGSIQAETSSSGAEAHR
                     KKYRPYGETLSSTGTSEPRGFTGQRQDASGLIDLHNRSYDPALGRFVSPDLVIDGENT
                     VGLNRYAYCNNNPVTRTDTEGTADEPEDKDKKDDKKAHGNWITRAADAAKHNGEFADE
                     PPTIGEPGQDKVRYDFYQKPVAVAGSHFKAVVTDESGKNVGTYSLAGWDAKYNDTNPG
                     DDNYKHAGTIYKDKESLKNFNDAYMNVADGNRYALGDSNAAIDHAIKAIGEDKGIFTL
                     SNPQHVPAAAYGYNNFDYQPPPEQKPPE"
     gene            complement(6707..7264)
                     /locus_tag="VFV99_15525"
     CDS             complement(6707..7264)
                     /locus_tag="VFV99_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060778.1"
                     /translation="MIVGRPLSEHTVDRHGLVLLGGFSTDGDNSAPRVDIFMPMPMES
                     APQFRIGLSLSYDSDSASIPDLSVQLYGAGIELLGAYDWRLPIATYSGDFVINVEGGP
                     SVGRIWMKVDMFDMPGEWDSVTGVGLGVASSVQYRAHNGFIASVQPIGIGIPIWHSDP
                     GANNPFTINSDVSWQGALMVGYQWR"
     gene            complement(7871..8548)
                     /locus_tag="VFV99_15530"
     CDS             complement(7871..8548)
                     /locus_tag="VFV99_15530"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5060779.1"
                     /translation="MAGVLSTGKAPRGGKRPNAGRKKSDDFKYDPSHAKRPDLFFKHP
                     VHVMLRVLSSVPRLRQRDGYEAIREALLYFVDGASFRVVHISIQRNHLHLVVEAAHKE
                     ALARGMQRFAIRAARALNAAFEREGKVFAFRYKAKQIKTAHYARNVIAYVLNNWRKHH
                     EDRRYEGMPFDRFTSAEAFAGWTTGDPGKPPDFGLPVSPPRTELLVFDWQLFGLIDPF
                     EVPGQVM"
     gene            9175..10488
                     /locus_tag="VFV99_15535"
     CDS             9175..10488
                     /locus_tag="VFV99_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5060780.1"
                     /translation="MRSRTLALLVLAAAPRFAAADPKKLSLDETITKALAGPKARMAR
                     GDADTAAGRLGEAKALRLPRIKGTFFGTASPKIDCLPPDCTRTDPENFAFRYEGLYVG
                     AYLDVEQPLFAPGKSHGLEAAKAGVEAQELLADEAAGDLAVDAARAYWGVKLAREMRF
                     MLEDGIDEIGKAIERMDARTGKDAPSPQDRQRVAVLLAEAKVQRADAKAAERQALAGL
                     RALTGVADADVDDDPLAALELPLPTNAAAKDRPQARAAKQGAVAADELVSFQTSYYYP
                     DLSLVGRGFATTAQGADDPPSAYAYDPYNRWGADLSLVVRWTAEPWSVNARVKRARAE
                     ARRAHALDDLAASGAQYDLETVLGEAEAAKEKLTAAADGEKAGRAWVVTLAQADVIGA
                     VESKDFADAYIAWFRMKANWALSAFQWNVAVVRIGRATGKYRAKL"
     gene            10883..11095
                     /locus_tag="VFV99_15540"
     CDS             10883..11095
                     /locus_tag="VFV99_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060781.1"
                     /translation="MKLKDRAREKAERRNERRDNPREQKGPEIAWDQAGGVGSVVRDN
                     PDPAPVAGGVTAPPTPATDDSTDTDT"
     gene            complement(11133..14015)
                     /locus_tag="VFV99_15545"
     CDS             complement(11133..14015)
                     /locus_tag="VFV99_15545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060782.1"
                     /translation="MSGRDVDALGATVGGAEAAPAPPRIGRGAIIDRYVVIDLLGAGG
                     MGQVFAAYDPELDRRVAIKLLRAGPGSTSREHQSRLMREGKAMAKLSHPNVVVVHDVG
                     THDSDMFVAMELVDGKTLEAWLKDKRNWREVVGAFVQAGRGLEAAHAAGIIHRDFKPA
                     NALVRVDGRVLVTDFGLARSVGAPHTDSDEPATEPHEPHVSSPSREATQAGTLLGTPA
                     YMSPEQLAGKPADARSDQFSFCLALYEGVFGVRPFDLPTISDTGEPDPNAMDEARWVI
                     PPLPHDCSVPMWLRRVIVRGLATDPKQRHASMSDLLRELSRDPARTRKVAAVVGVGVA
                     LAGAGIAWGATRTQTKACEVPTNAAIDAVWNAGAKAKMKAAFEATKRPYAADTFARVA
                     GELDERTTQWRGAWVSACEATEVQHAQSANLLDLRMQCLDRRAKEIGAQVDLFTRAEA
                     DIVDKAVKAVAVLPDLSECANAERLSTRVPLPTDPMLRDKIAAVRTKLDTELAQFRAG
                     KAKALAPTAVALADEAAALGYPPLEAEARYRTSDIFNELGDAKISEEQLRKALPAAAA
                     SRDDILLSRVVNGLVFALGHQEKRYLEADALVPAAEALATRADDEARIAELAYYRGII
                     DEDRGHYKEARALLEQALTMRTKLVGEDHPEVAVIHQNLAIVLDALGEYDTGRGHSER
                     TYAILEKTYGPEHPAVGTARLMVGGSLRRAGKLQEAKPHFEAALRIWTAAYGPDHPYV
                     GMGLNNLLVILDDLGEQEEALKVGLRALEFKEKHSGPESEATAATLANLGRLLRSMHR
                     NPEARPYLERALTIRRKVLEANHPDIATSLSGLALLELEEKRPAEAQKLAIEALAIRE
                     KLGPEHPDLLTELLLLAKTHVKLGKPAAAIPLLERALAIAAKKTVDASTAAEVKLDLA
                     KALWDRGDKKRAVTLVTEARAAWAEVGAAAKEPLAECDAWLAAHKI"
     gene            complement(14101..15015)
                     /locus_tag="VFV99_15550"
     CDS             complement(14101..15015)
                     /locus_tag="VFV99_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060783.1"
                     /translation="MRALGIVLLVCALCGHAAAQSPEDIEKANAIFLEGRELLTSKQT
                     KAACEKFEESIALDPTAPGVMLNLGLCYELLEQYATSLYWFRKAQVAAAEAHLPAYED
                     EAKRHTLSLAAKVSVVKLTVPEGAEVLIDGKLIAPTDYARVEVDRGTHNLEARADGKQ
                     TYRHEFEVDAIDAGTIKIPELAAAGGGGGGQNVIVGPGPDTSAPSTLGRTVMAATFGG
                     AGLVLCIASPLWARHTKTAYDDAVAKGEMPSYSSARTKQHIATGLFIAGLGAIGVGTY
                     LYLTMPSGTTVAPVVDAQQVGVVISGSL"
     gene            complement(15026..15481)
                     /locus_tag="VFV99_15555"
     CDS             complement(15026..15481)
                     /locus_tag="VFV99_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060784.1"
                     /translation="MVDNRLEASEHTSLWPKTRRGAVLYALAAMVAVVVGVIPALWIN
                     RWRSDADAAPAKQAAPVVMPTVEPIEAEAVPTEQPPAPEPTVPTVPAVDQATIDMDAA
                     TAPVATAKTTKRPRPKTARPKPAATKPAPTKPKPPPCDIYLHPHGCPNG"
     gene            complement(15550..16317)
                     /locus_tag="VFV99_15560"
     CDS             complement(15550..16317)
                     /locus_tag="VFV99_15560"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HEX5060785.1"
                     /translation="MWRACLLLLFASGCHIVFPIEPYDSGDDDGGLTGNAPKVFSSPG
                     PAASLYGGYPKSYAITLAADQPGTMIYYTTDGSEPSVDSTATMFAVTPIKGITISTTT
                     TIKYFGVSGGTQSPTTTETMSIDATTAQINAGYLVTNVTLDGTSPVVIAAGGSILAAR
                     ANVQTWVQNNCAACAAQVVYGIDQTDQGCLFDGGAGVYPGMTFNNKAFNVTVPSTPGV
                     HEVKLAHIEQTSCAAAMAVGALKTRPTLARIGVIIVR"
     gene            complement(16330..>16882)
                     /locus_tag="VFV99_15565"
     CDS             complement(16330..>16882)
                     /locus_tag="VFV99_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5060786.1"
                     /translation="GATVVVFGLGGIGLNVVQGARMAGATMIVGVDINPAREAAGRAL
                     GMTHFVNPKDHGDKLVGALIELTGGGADYSFECVGDVKLMRQALECCHRGWGVSVIIG
                     VAGAGQEISARPFQLVTGRVWKGTAFGGARGRTDVPRIIDWYMDKRIEIDSMITHKLP
                     LDRINDAFDLMHAGKSIRAVVEY"
BASE COUNT         2658 a         5522 c         5566 g         3136 t
ORIGIN      
        1 atcggcctcg tgcaagttgg tcagcgactc gacgagagcg gcaacgtgcg catcgggcac
       61 gttgacgttg ccgacgacga acgaacctcg tctgcgctcg gctaacaggg atagacgctt
      121 atcgtcgggc gagtgaccgt actgaactgc gagtcgttcg acgtgttcgg cttggtcgac
      181 ttgttccact cgttcggctc gttcgccgga ctcgcacgcg aacgccacaa acgagagcag
      241 cgcggccgac atttgccagc aacgtgtgcg agcacctttc aaacggctgt cgctcatgtc
      301 gggtatagat cgcgcgagac gtggctatta tgatcgatcg tcctcgtcgg agcgcggata
      361 cagtcgagct ccactttacg attctgagag gtgcatctct tgtgcttcca tgcacgtact
      421 cgagtacatc ggcaccacta acggcagcgc gaggctacag gcgggcgcac gcatagcgat
      481 cacctgtgtc ggggtcgtgt tcgttggaga gctgcgcgcg tcgcaagatg cgagcggagc
      541 gcggctcgtg gccctcggcg attcgacggt cacctccggc cgatacggac actgcaagct
      601 ctcgatcgtg gatggccaag tcgttttgta cgactgcggc tcggacaacg gcacgtggat
      661 cgtgcacggc gacacgttcc agcgagtcgg ctcgatcgct tcaccaacga cgatcgcagc
      721 cggcaccgaa atcgccatcg ggcgttctcg gttccgggtg ctcgacatac cggactgagg
      781 acgagcagct gacgtgttgc ttattccggc ggcttctgct cgggcggcgg ctgatagtcg
      841 aagttgttgt agccgtacgc cgcggccgga acgtgctgcg ggttggacag cgtgaagatg
      901 cccttgtcct ccccaatcgc cttgatcgcg tgatcgatcg cggcgttcga gtcgccgagc
      961 gcgtaccggt tgccatcggc gacgttcatg tacgcgtcgt tgaagttctt gaggctctcc
     1021 ttgtccttgt agatcgtgcc ggcgtgcttg tagttgtcgt cgccggggtt ggtgtcgttg
     1081 tacttggcgt cccagcccgc gaggctgtac gtgccgacgt tcttgcccga ctcgtcggta
     1141 acgaccgcct tgaagtgcga accagcgact gcgaccggct tctgatagaa gtcgtagcgc
     1201 accttgtcct ggccgggctc gccgatggtc ggtggctcgt cggcgaactc gccgttgtgc
     1261 ttcgccgcgt ccgcagcacg cgtgatccag ttgccgtggg ctttcttgtc gtccttcttg
     1321 tccttgtcct cgggctcgtc ggccgtgccc tcggtgtccg tgcgcgtaac cgggttgttg
     1381 ttgcagtacg cgtagcggtt gagaccgacg gtgttctcgc cgtcgatcac gagatcgggt
     1441 gacacgaagc gaccgagcgc cggatcgtac gaacggttgt ggaggtcgat gagaccagag
     1501 gcgtcctgcc gctgaccggt gaagccgcgc ggctcggacg ttccggtgga cgacagcgtc
     1561 tcgccgtacg ggcggtattt tttgcgatgt gcctcggcgc ccgacgagct ggtctccgcc
     1621 tggatcgagc cgagatgatc ggtgtgcacc cagtacgacg tggtgccatc gcggcgcgcg
     1681 accgtgatgt cgccgaccgt gacgtacttg ctcgcgttgc cgccgacctg gatctcgaag
     1741 ttgtcgccga ggaatcggcg cgtgatgccg ttctcgattt gctgcacgcg ggcgccatcg
     1801 gcgtcgtacg tgtaggcgac gttcgcgggc ccgctgatcg tggccagtcg gttgtcgccg
     1861 tcccaggtca gcgtgcggcc accgcccgcg gtcatgagac cggcggcatc gtagctgtac
     1921 gtgttcgagc ccgccgcggt gactgcatgc ggacgcgacg tgccgtacgt gtagctgccg
     1981 agccgagagt tggccgtgat gttgccgatc gcgttgtacg tgaacgtctg gttttgcgtc
     2041 gcgtcggacg tgttggtcgc cgagagcaag cgatcgagct cgtcgtacgc gtacgtccag
     2101 ccctcgttcg cgatcgggct cgtcacgctg gtgatgaggc ccctcgcgtt gcgcgtgtag
     2161 ccgaggttct ggatcgtggt cgcgccggct gtcgtcgaga tgcgggtgag ccagccgcgc
     2221 tgcgccgagt acgtccgcgt gttgaccgca ccattcgcgc tgtcgattcg cgccaccttg
     2281 tcgtcggcgc cgtagagcac cgaggtgacg taaccgggga tcgatgcgag ccggcctgcg
     2341 gagtcgtaga cgagcggcgt gcccggcgta ccgagcgtgt caccgtcggg ataggtcgtc
     2401 cacagcgcgc gaccgcccgc gtcgaagccg cgcgtgaacg tgtaactcgt tccacctgtc
     2461 acgcgcacgg tcttcaccga gcgaccggcg gcatcgtagt cgagcgtctt gctgccggcg
     2521 ctgtcggtca tcgccgtcaa cttgcccttg ttgaagtagc cggtgcggac ttgatcgtag
     2581 gtccagctca ccgtgaccgc tgagcccgtg cccgccttgc tcgtcttgcc ggtcttgcga
     2641 tcgagcgcgt cgtacgtgaa gtccgtgcgc tgagccttcg cgtccgtttg cgacgtgagc
     2701 cggttcgcgc catcgaacgc gtagctggag gcgcccgagt ccggatcggt catcgccgtc
     2761 gtgcgagcga gcgagtcgtt cacgtagttg atgctgttgc cgctcggatc ggtagatcgg
     2821 atgaggttgc cgcgcgcgtc gtagaggtac gccgtgacct ggtacgcacc accgacgtat
     2881 tccttgtgcg tcacgcgccg gccgtctgcg tctttgatgt tgatcatgct gtggccgagc
     2941 tcgtcggtct cggtcgtcgt ccacaggctg tagctcgtgg tcttgaagct gccgtccgcg
     3001 tgcgtgacct tgatcggacg gtcgagcgcg tcgtagtcgg tcgtggtcgt gtaggtcgtc
     3061 ggttgcggtc ccgacgccca gtagtacgga cgcgtcttcg acctctgctc gccgcgcgcg
     3121 ttgtatccgg tgtcgacgta gatgttcccg ctcgcggccg ggccctgttt cacggtgcgc
     3181 cagatgcgct tgtggccgtc gaggtactcg cgccgccaca tcgcggtagc accgccgtcg
     3241 gcggcaggcg tttcgatctg ctcgtactgg ctcgtcgcat caccgaggcc gagccacgtg
     3301 tgacgctcga agttgccgcc cggctcggtc ctctgcgaga ggcgacacag cgggtcatag
     3361 gtgagcgcgg tcgtctgact gttgagatcg acgagctgag atttctcgcc gcagacggtg
     3421 ttccagctcg tcgtggtcac ctggctgagc gcgttggtct ccgacgtcgc gaagatgtgg
     3481 taggtcgcat cgaacgcggt cgtcgtccgc gcaccgagcg cattgatcgt cgcagtcacg
     3541 ttgccgtacg tgtcgtattc cttgctcgtg ttgacgaacg agctcggact cgacagccac
     3601 cgcgactctt tggtcgggtt gccgaccgac ggagcctggt tccaggtggc cgcaccgtcg
     3661 tagtaggtca gcgtttcgtt gaggagcgtg ccggccgtac cgacgcctgc gaacgacttc
     3721 acgtcggccg gcttgttcac gatgtacgcg gtcgtgttgg gcacgaacgt gttcgattgc
     3781 gtgtcctcgt cgccggtcgt gtcgtagtcg ccgtactcga cctcggtgac ggcgtcgccg
     3841 tacgcgttga acgtgcggct cacgtatttg cgcttgcagt cggcgcccgg gcacgcggta
     3901 cccgcgttga tgaacgtgta gtcccaattg ccggtgcgca gcgacgtcca cggcaccgtg
     3961 gcgccgttgg tcgtgtactc gtagatctcc gacgacagga gctggccgtt gcccgcgcga
     4021 tgatcgatgc gttcgggctt cgacgccgag ccatagtcct ggcgaaacca ggtctcgttg
     4081 tacgggcaca ccgactcgcc ggtgatgcac ggccgcgtct ccttctcgta gcgaaagccc
     4141 atgaaccgat gctcgatccg atcgtagagg ccaccgctgt acgtgtagtt ggtggtccag
     4201 acgccgccgc ggccatcgtt gagcgtgatc gacgccgccg tctgcatgag cggtgggttg
     4261 ttcgtgttgg tccatgccga cgaaggcgcg tacgccacgc ttgtcgtcgc gcccaagctg
     4321 ccgcggatcg aggtcatcag gtcggggtac ggcccggtca tgttccagat gcgccgcgac
     4381 cggattccga agaacgaatc catcgtgacc atctcggaga gtccgtcgcc gttgacgtcg
     4441 gcggcgagca gcttcgtgcc atcgccgaac gccgactgcg tatccaccgc gccaccgaca
     4501 aagttgtcgc cgaccgacag ccagatcttg cgctgctcga tgccgaggcc gacagactcg
     4561 atcgtgatca gatccgatcg accgtcgccg ttgacatcct cggcgaggaa ccagtcgccc
     4621 gcgtcataga acggcacgct gacctcggag acttcggtga accccttgcc cgtcgagtgc
     4681 cacacgcggc ggacgcggtt gccgaagaac gacgcgagat tgacgaggtc gtccttgccg
     4741 tcgccgttga cgtccatcgc caagaacggc gaccacgcgg tgaccacgcc gtccttctgc
     4801 gcctccacga cttggccatc gcattggtcg gtgactccgg tcgcgaagcc ggtgccggtg
     4861 gagatccagg tcgtgcgttg aactgcgctg aagtagtcct tgagctggac catgtccatc
     4921 ttgccgtcgc cgttcacgtc gagcgtcagg acgagatact tcgggtcggt gtcgaagttc
     4981 agcgtctgtc cctcggtgaa cgcggtgccg ttcgagaacc acacgcgacg cccctccgcc
     5041 ccgaacaaca cgctcgggta ctgctcgacg agatcggtct tgccgtcgcc gttgacgtcc
     5101 agcgcgaagt agcgcgagtc cgcggacgtc gtcttcatgc agcatgcatc cgacgcttgg
     5161 ttgaagcgcg tgccatccga cagccatacg cgacgccgct ccgaccccca gctctcgtac
     5221 agctcgatga tgtcgccctt gccgtcgccg ttgacgtctg cagtcagaaa ccgtgtcgtc
     5281 gtggccgtcg ccatcccgct gtcgctcgag ctctgtacaa acccgctgcc cgtcgagatg
     5341 tgggcgacgc ggtagcgcgt gcacagaatg aaacacgcgt tgtagatctc gagcatgtcc
     5401 gtcttgccgt cgccgttgat gtcgatcgcg aagtagtccg catcggtgtt gttactcaca
     5461 ccgttgtcgg tggtgcccga tacgaacgac ggcgcgccgc tctggtactg catcgtgatc
     5521 gccggcaggc tcgtgccgcc ggtgaccgtt cccgtaccgt ccagcgtcgc gtcggtgccg
     5581 aactgctgca cgctcgtcag tagcgagcgc gacgtcgagc cactcgtccc atacgacagc
     5641 ttgtacgcgc gcacgcggct gccactcacc gtcacgtcga tcgtcttgat gcggccgaac
     5701 acgttccgga cgccgacacc aaccgcgttg ctcgggcgat ccgtgcgctg ctcgtaatag
     5761 aacgtgacgc tggtgccgtt gtagctgacc gactgcggat actcccagca gcaaccgaat
     5821 tgattgacgg cccacgtgta gttgaccgtg ttgccgcgcg tgtcgatgac ctgcgagagg
     5881 ccccatcggt agacatcgac gttgttgttg acggtgtaca caggcgcgta aatgcgacgc
     5941 gtgccgtcct tctgggtcac ggtccagcga ctcgcggtgc cggtaccggt gagcgcgatg
     6001 cgcaggtaat tctcgacctt cgtcgagtgc gtgccgccgc tcgtgcagct cgggctcacg
     6061 ctcccggcga cacatgcaac gagctcttcg ccgtcgagca agaagatgtc gctcgcgttg
     6121 tacttcggtg cgcctttgcc cgggctcgcg cgctcgacga tgccgatgcc ttcgaggctc
     6181 cagccgacgc cgacccagcc attgccgtcg gacgagtcgt acttgagacc gaggtttggc
     6241 gtgaggtcgc gaaacgcagg gacttcgacg cgaatctgct cgctgaacgt gccgtgcgat
     6301 gcatcgacca cccacgtctt cgccgtggtg tccttcttgt ccgtcttggc gttcgccgtg
     6361 ccgaacgtac cggcgacaag gcatacgacg agcagatcgc tgcgcttgga catagctccc
     6421 cctgtccgac gctgtccctc gcgtcggcga actcgaacgt attcgaagcc acgcgccagg
     6481 gcgctacgat cccgtccctc gcgcgagata aacacgttat atacctcacg cacgagagtc
     6541 aacacgaatg aatttcgcgt gttgttcaga gtctgaacga actgttcaga tccagttcgc
     6601 acgatgcgaa gcgcgcgcgc gaaagcgctc ctatggttgg accggcgcta actccggaag
     6661 cgcgcgatcg cagtcgcggg accacttggt caggcgggca cgaggatcag cgccactgat
     6721 agccaaccat cagcgcgcct tgccacgaca cgtccgagtt gatcgtgaac ggattgttgg
     6781 cgccaggatc cgagtgccag atcggaatgc cgatgccaat cggctgcacc gacgcgatga
     6841 agccgttgtg cgcgcggtac tgcaccgacg acgcgacgcc gagcccgaca ccagtgacgc
     6901 tgtcccattc gccgggcatg tcgaacatgt cgaccttcat ccagatgcga ccgacgctcg
     6961 gaccgccttc gacattgatc acgaagtcgc ccgagtacgt cgcgatcggc aggcgccagt
     7021 cgtaggcgcc gagcaactcg atgcccgcgc cgtagagctg caccgacaga tcgggaatcg
     7081 atgcggagtc ggagtcgtag ctaagcgaga ggccgatgcg aaactgcggc gcgctctcca
     7141 tcggcatcgg catgaagatg tcgacgcgcg gcgcgctgtt gtcgccgtcg gtcgagaagc
     7201 cgccgagtag cacgaggccg tggcgatcga cggtgtgctc cgagagcggg cggccgacta
     7261 tcatcggctg ctgcgtcccg tagccaggct gaccgtacgc aggctgttga ccgtacgcag
     7321 gctgttgacc gtacgcagcc ggttggccat tcgcaggcgg ctgaccatat gcaggcgctt
     7381 ggccattcgc aggctgctga ccgtacgcgg gcgcttgacc attcgcaggc tgctgaccgt
     7441 acgcgggcgc ttggccattc gcagatgcgg atgcgcctgc cgtctgcggg gctgcagctc
     7501 taggcggcgc ggacgttggt gtcccaggtg ctggcggcgc ggctggcgcg ggtgcctgtg
     7561 cacgcgcgat gtgagtcgac agcaccacca cacaccccgc gagtgcaatg aaacgcatcg
     7621 cgaccattct aggtgttcgg ggctggcgtc gacagcagca attggttgca gttcgctgtg
     7681 tgacgcgatc gcacgggcac agtgtgggcg tcttgcgtcc acgcagtttc tgcaaatcgt
     7741 gggcggagcc acagcagctg ctctgcacgg gaacaggtgc agcgattgtg gcaatgcgcg
     7801 agagtcgtag tccggccatg aagcgcgagc accgccgctg gccgctaacg aaggcctggc
     7861 cgggtgtgcg tcacatgacc tgcccgggaa cttcgaacgg atcgatgagc ccgaacagtt
     7921 gccaatcgaa cacgagcagc tccgttcggg gtggcgagac cggcaagccg aagtctggag
     7981 gctttcctgg atcgccggtg gtccagcccg caaacgcctc ggcgctcgtg aagcgatcga
     8041 acggcatgcc ctcgtaacgg cgatcctcgt gatgcttgcg ccagttgttc agcacgtacg
     8101 cgatgacatt gcgcgcgtag tgcgcggtct tgatctgctt cgccttgtag cgaaacgcga
     8161 acaccttgcc ttcgcgttcg aacgcggcat tgagcgcacg cgccgcgcgg atcgcgaacc
     8221 gctgcatgcc gcgggcaagc gcctccttgt gcgcggcctc gactacgagg tgcaagtgat
     8281 tgcgctggat cgaaatatgg acgacgcgaa agctcgcgcc gtcgacgaag tacagcagcg
     8341 cctcacgaat ggcctcgtag ccgtcgcgct gacgcaggcg cggcacggag gagagcacgc
     8401 gcagcatgac gtgtaccgga tgcttgaaga agaggtccgg tcgtttcgcg tgcgaagggt
     8461 cgtacttgaa gtcgtccgac ttcttgcgac ccgcgttcgg ccgcttgccg ccacgcggcg
     8521 cctttcccgt cgacaaaaca cccgccattt gatttggcag taacatgtcg ctctgacatc
     8581 gagttgtcga gcaaaccgat cgcgaacgcg aggtatcgtt gtgggccgga gctcatcgtt
     8641 gaacggcacg cgctcgagat cggaagcatc tcgcgatcgg atcgccgtgt tccgcgcacg
     8701 gcatcgcacg ccgcgatcgg cagctgattc gcgcccggat cggaggcacg tcgcgatcag
     8761 atcacggttg atccgcaccc gcatcggagg ccccctgcga ttggatcgtc gctgatccgc
     8821 gcgcccgaca tcggacacag gtcgcgacgg cgctgatccg cgcgcgcgac atcggatcgc
     8881 tgttgatccg agcactcgat atcggaagcg ccttggtatc ggctcgccgt tgatctgagc
     8941 gctcgacacc ggatgcgggt cgctatccga tcggcggggt tccaccagtt ggccgaggcg
     9001 tcgctatcga atctggtcga gaccgcgaac gccgcccgcc tgcccgcgcg accccgcata
     9061 cgtgttcgta ccgctattgc ccacgtcttc cgcgaaacca cgcgataccg gtgccgcccg
     9121 cattgtcttc cgcgaaacca cgcgatgcac gtggcccaca gggtaagaat acccatgcgc
     9181 tcgcgtaccc tcgcgcttct ggttctggcc gccgcccctc gattcgcagc ggccgatccg
     9241 aaaaagcttt cgctcgacga gacgatcacc aaggcactcg ccggcccgaa ggcgcgcatg
     9301 gcgcgcggcg acgcggacac tgctgctggg cggctcggcg aagcgaaggc gctgcgcttg
     9361 ccgcgcatca agggcacgtt cttcggtacg gcgagcccga agatcgactg cctccccccg
     9421 gactgtacga ggacggatcc ggagaacttc gcgttccgct acgaagggct ttacgtcggt
     9481 gcgtacctcg acgtcgagca gccgctgttc gcgcctggca agagccacgg tctggaggca
     9541 gcgaaagctg gcgtcgaagc gcaggagcta ctcgccgacg aagcggccgg cgatctggcc
     9601 gtcgacgccg cacgcgcgta ctggggtgtg aagctcgcgc gcgagatgcg cttcatgctc
     9661 gaagatggca tcgacgagat cggcaaggcg atcgaacgca tggatgcacg cacgggcaag
     9721 gacgcgccgt cgccgcaaga tcggcaacgc gtggcggtgc tgctcgccga ggcgaaggtg
     9781 cagcgcgccg atgcgaaagc ggcggagcgg caggcgctcg ccgggctacg cgcgctgacc
     9841 ggcgtcgccg atgccgatgt cgacgacgat ccacttgcgg cgttggagct accgctaccg
     9901 acgaacgccg cagcgaagga ccgcccgcaa gcacgcgccg cgaagcaagg cgccgtcgcc
     9961 gccgacgagc tcgtctcgtt ccagacctcg tactattacc ctgacctctc gcttgtcggt
    10021 cgcgggttcg caacaacggc acaaggcgcc gacgatccgc cgagcgcgta tgcctacgat
    10081 ccgtacaatc gctggggcgc cgacctctcg ctcgtcgtgc gctggaccgc cgagccgtgg
    10141 agcgtgaacg cgcgcgtcaa gcgtgctcgt gcggaggcgc gccgcgcgca tgcactcgac
    10201 gatctcgctg cgtcgggcgc gcagtacgat ctcgaaaccg tgctcggcga agccgaggcc
    10261 gcgaaggaga agctgaccgc cgcggcagat ggcgagaagg ccgggcgcgc gtgggttgtc
    10321 acgctcgcgc aggcggatgt gatcggtgcg gtcgagtcga aggacttcgc cgacgcgtac
    10381 atcgcgtggt tccgcatgaa ggcgaactgg gcgctgtctg cgttccagtg gaacgtcgcc
    10441 gtcgtgcgga tcgggcgcgc cacaggcaag taccgcgcga agctctagtt tgcaggagga
    10501 tgcgtgacgg acagctggac cgagcggcag ttgaacgtct gtgagcaata cggcgtgtcg
    10561 tcgattccat gtcgacccga cgagaaggtc gggatcgcac ggaatgttca gaccggattg
    10621 ctcccagtca acggcgcgcg ccacctagcc cggcaaagtt ctagcgaatg caatcgctac
    10681 gacctcttcc gaggttttgt catgccggcg gcggcgtggt atccaatcac actaaatcca
    10741 cactaaatct cgacgtggac gggcgaacgt cgtcgctccg cccgcttcgg cgcccaactg
    10801 ggctgtatta cgacgggcgc cccgagcgga aaggacctga atggcaaatc aacgaggaac
    10861 cttcgccaaa cgaaaccgcg agatgaagct gaaggaccgc gcgcgcgaaa aagcagaacg
    10921 tcgcaacgag cgccgtgaca atcctcggga gcagaaaggc cccgagatcg cgtgggacca
    10981 ggcgggtgga gtcggcagtg tggtgcggga caatcccgat ccagcacccg tggcaggtgg
    11041 cgtgaccgcc ccgcctacgc cggccaccga cgacagtact gacaccgata cgtagttccg
    11101 agcgaccggc tcgatcccga tcggctcgag gactagatct tgtgtgccgc gagccacgcg
    11161 tcgcactcgg cgagcggctc cttcgcggct gcgccaacct cggcccacgc cgcgcgcgct
    11221 tcggtgacga gcgtgacggc gcgcttcttg tcgccgcgat cccacagcgc cttcgcgagg
    11281 tcgagcttga cctcggctgc cgtcgaggca tcgaccgtct tcttcgccgc gatcgcgagc
    11341 gcacgttcga gcagcggaat cgccgccgcc ggcttgccga gcttgacgtg cgtcttcgcg
    11401 agcagcagca gctcggtgag taggtccgga tgctcgggcc cgagcttctc gcgaatcgcg
    11461 agcgcttcga tcgcgagctt ctgcgcctcg gccggccgct tctcttcgag ctcgagcaat
    11521 gcgaggccgc tcagcgacgt cgcgatgtcg ggatggttgg cttcgagcac cttgcggcga
    11581 atcgtgagcg cccgctcgag gtacggccgc gcttcggggt tacggtgcat gctgcggagg
    11641 aggcggccga ggttcgcgag cgtggcggcg gttgcttcgg attcggggcc gctgtgcttc
    11701 tccttgaact cgagcgcgcg caggccgacc ttgagggctt cctcttgctc gccgagatcg
    11761 tcgaggatca cgagcaggtt gttgaggccc atgccgacgt acggatgatc gggcccgtat
    11821 gcggcggtcc agatccgcag cgcggcttcg aagtgcggct tcgcttcctg cagcttgccc
    11881 gcgcggcgta gcgagccgcc aaccatcagc cgcgccgtac cgaccgccgg atgctccggt
    11941 ccgtacgttt tctcgaggat cgcgtacgtg cgttccgagt gaccacgacc ggtgtcgtac
    12001 tcgccgagcg cgtcgagcac gatggcgagg ttctggtgaa tcaccgcgac ttcgggatgg
    12061 tcttcgccga cgagcttcgt gcgcatcgtc agcgcctgct cgagcagcgc gcgcgcttct
    12121 ttgtagtgtc cgcgatcctc gtcgatgatg ccgcggtaat acgcgagctc ggcaatgcgc
    12181 gcttcgtcgt cggcacgagt cgcgagggcc tctgcggccg ggaccagcgc atccgcttcg
    12241 aggtagcgct tctcctggtg cccgagtgcg aacacgagcc cgttcacgac gcgcgagagc
    12301 agaatgtcgt cgcgcgatgc ggctgccgcc ggcagggcct tgcgcagctg ctcctcggag
    12361 atcttggcgt cgcccagctc gttgaagatg tcgctcgtgc gatagcgcgc ctcggcttcg
    12421 agcggcggat agccgagcgc cgcagcttca tcggcgagcg cgaccgccgt cggtgcgagc
    12481 gcctttgctt ttccggcacg gaattgcgcg agctcggtat cgagcttcgt gcgcaccgcc
    12541 gcgatcttgt cgcgcagcat cggatcggtc ggcagcggca cgcgggtcga gagtcgctcc
    12601 gcgttcgcgc attcgctcag gtccggcagg acggccaccg ccttcaccgc tttgtcgacg
    12661 atgtcggctt ctgcgcgcgt gaagagatcc acctgcgcgc cgatttcttt cgcgcgccga
    12721 tcgaggcact gcatgcgcag atcgagcagg ttcgccgact gcgcgtgctg cacctcggtc
    12781 gcctcgcatg ccgagaccca cgcgccgcgc cactgcgtcg tgcgctcgtc gagctcgccg
    12841 gcgacgcgcg cgaacgtgtc ggcggcgtac ggccgcttcg tcgcttcgaa tgccgccttc
    12901 atctttgctt tcgcgcccgc gttccacacc gcatcgatcg ccgcgtttgt aggcacctcg
    12961 catgcctttg tttgcgtgcg cgtcgcgccc cacgcgatgc ccgcacccgc gagcgccaca
    13021 ccgacaccga cgaccgctgc caccttgcgc gtgcgcgccg gatcacgcga cagctcgcgg
    13081 aggaggtccg acatcgacgc gtggcgctgc ttcggatccg tcgcgagacc gcgaacgatc
    13141 acccggcgca gccacatcgg caccgagcag tcatgcggca gcggcgggat tacccagcgc
    13201 gcctcgtcca tcgcgttcgg atcgggctcg ccggtgtcgc tgatcgtcgg caggtcgaac
    13261 ggacgcacgc cgaacacgcc ttcgtacagc gcgaggcaga agctgaactg atcggagcgc
    13321 gcgtcggcgg gcttgcctgc gagctgctcg ggggacatat acgcgggcgt gccgagtagc
    13381 gtgcccgctt gcgtcgcctc gcgactcggc gagctcacgt gcggctcgtg cggctcggtg
    13441 gccggctcgt cgctgtcggt gtgcggcgcg ccgacgctgc gcgcgagacc gaagtccgtg
    13501 accagtacgc ggccatcgac ccgaacgagc gcgttcgccg gtttgaagtc gcgatgaatg
    13561 atgcctgcgg cgtgtgcagc ctcgaggccc ctgcccgctt ggacgaatgc gccgacgacc
    13621 tcgcgccagt tgcgcttgtc tttgagccag gcctcgagcg tcttgccgtc gacaagctcc
    13681 atcgcgacga acatgtcgct gtcgtgcgtg ccgacgtcgt ggacgacgac gacgttcgga
    13741 tgcgacagct tcgccatcgc cttgccttca cgcatgaggc gcgactggtg ctcgcgtgac
    13801 gtgctgccgg gacctgcgcg gagcagcttg atcgcgacgc gacgatcgag ctcgggatcg
    13861 taggcagcga atacctggcc catgccgccc gcgccgagca gatcgatcac gacgtagcga
    13921 tcgatgatcg cgccgcggcc aatgcgcggt ggggccggcg ccgcctcggc accgcctacc
    13981 gttgccccca atgcatcgac gtcgcgaccg ctcaccgccg tccatgataa cgcgaacgct
    14041 atgggagcgg gatcgcccag acgacgtgat cgtccacacc gcaccgctga cctgcggcga
    14101 ctagagcgag ccgctgatca cgacgccgac ttgttgcgcg tcgacgaccg gcgcgaccgt
    14161 cgtgccgctc ggcatcgtca gatacagata tgtgccgact ccaatcgcac cgaggcccgc
    14221 gatgaacaat ccggtcgcga tgtgttgctt cgtgcgcgcc gacgagtagc tcggcatctc
    14281 gcccttcgcg accgcatcat cgtatgcggt tttcgtgtga cgcgcccaca gcggcgacgc
    14341 gatgcacagc acgaggcctg ccccgccaaa cgtcgctgcc atcaccgtgc gcccgagcgt
    14401 cgacggtgcg ctggtatccg ggcccggccc gacgatgacg ttctgcccac cgccaccacc
    14461 gccggcggcg gccagctccg ggatcttgat cgttccggcg tcgatcgcgt cgacctcgaa
    14521 ttcatgccga tacgtctgct tgccgtccgc tcgcgcttcg agattgtgcg tgccgcgatc
    14581 gacctcgaca cgcgcgtagt ccgtcggcgc gatcagcttg ccgtcgatca gcacctccgc
    14641 gccctcgggc acggtcagct tcacgaccga caccttcgcg gcgagcgaca gcgtgtgacg
    14701 cttcgcttcg tcttcgtagg cgggaagatg cgcctcggcc gccgcgacct gcgccttgcg
    14761 gaaccagtac agcgatgtcg cgtactgctc gagtagctcg tagcagaggc cgaggttgag
    14821 catcacgccc ggcgcggtcg gatcgagcgc gatcgattcc tcgaacttct cgcatgcagc
    14881 cttcgtctgc ttgctcgtga gtagctcgcg gccttcgaga aagatcgcgt tcgctttctc
    14941 gatgtcctcg ggcgactgcg cggccgcgtg gccacacagt gcgcacacga gaagaacaat
    15001 cccgagtgcg cgcatcgccg ctagtttacc cgttggggca gccgtgcggg tgcaagtaga
    15061 tgtcacacgg cggcggcttc ggcttcgtgg gcgccggctt tgtcgccgcg ggcttcgggc
    15121 gcgccgtctt cgggcgcggc cgcttcgtcg tcttggcggt ggccacgggc gctgtcgctg
    15181 cgtccatgtc gatcgtggct tggtcgacgg ccggcaccgt cggtacggtt ggctccggcg
    15241 caggcggctg ctcggtcggc accgcctccg cctcgatcgg ctcgaccgtc ggcatcacca
    15301 ccggcgcggc ctgcttcgcg ggagccgcat cggcatccga gcgccagcgg ttgatccaca
    15361 gtgccgggat cacgccgacc acgaccgcca ccatggccgc cagcgcgtag agcaccgccc
    15421 ctcgcctggt ctttggccac agggaggtgt gctcgctggc ctcgaggcga ttgtcgacca
    15481 ctgcggcaag ggtaacgtgc ccgggctaac ggctcaattg ccgtctaata cgccaaggca
    15541 gcgtttctcc tagcggacga tgatcacacc gatgcgagcg agcgtcggcc gggtcttcag
    15601 ggcccccacc gccatggccg ccgcgcagct ggtctgctcg atatgggcga gcttgacctc
    15661 gtggaccccg ggcgtcgacg gcaccgtgac gttgaacgcc ttgttgttga acgtcatccc
    15721 cgggtagacg cccgcaccgc cgtcgaacag gcagccctga tcggtttggt cgatcccata
    15781 gacgacctgc gccgcgcagg ccgcacagtt gttctgcacc caggtctgca cgttcgcacg
    15841 tgccgcgagg atggagccgc cggctgcgat caccacgggc gacgtgccat cgagcgtgac
    15901 attcgtgacg agatagcccg cattgatctg cgcggtcgtc gcatcgatcg acatcgtctc
    15961 ggtcgtcgtc gggctttgcg tgccgcccga gacgccgaag tacttgatgg tcgtcgtcgt
    16021 cgagatcgtg atgcctttga tcggcgtcac cgcgaacatc gtcgccgtcg agtcgacgct
    16081 cggctcactg ccgtcggtcg tgtagtagat catcgtgccc ggctggtcgg ccgcgagcgt
    16141 gatcgcgtac gacttcggat agccgccata gagcgacgcg gctggcccgg gcgacgagaa
    16201 caccttcggc gcgttgcccg tcagcccgcc gtcatcgtcg ccgctgtcgt acggctcgat
    16261 cgggaacacg atatggcacc cgcttgcgaa caacagcagc aggcaggcgc gccacacgcc
    16321 cctagtctat cagtactcga cgacggcgcg gatcgacttc cctgcgtgca tgaggtcgaa
    16381 cgcgtcgttg atgcggtcga gcggcagttt gtgcgtgatc atcgagtcga tttcgatccg
    16441 cttatccatg taccagtcga tgatccgcgg cacgtcggtg cggccgcgag ccccgccgaa
    16501 agccgtgcct ttccagaccc gcccggtgac cagctggaac gggcgcgccg agatttcctg
    16561 gcccgccccc gccacgccga tgatcacgct cacaccccag ccgcgatggc agcactcgag
    16621 cgcctggcgc atcagcttga cgtcgccgac gcactcgaag ctgtagtccg cgccaccgcc
    16681 ggtgagctcg atcagcgcgc cgacgagctt gtcgccgtgg tccttcgggt tcacgaagtg
    16741 cgtcatgccg agcgcgcggc cagccgcctc gcgcgcgggg ttgatgtcga caccgacgat
    16801 catcgtcgcg cccgccatgc gtgcgccctg cacgacgttg aggccgatgc cgccgaggcc
    16861 gaatacgacg acggtcgcgc cg
//
LOCUS       DASUFX010000389         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1174189, whole genome shotgun sequence.
ACCESSION   DASUFX010000389 DASUFX010000000
VERSION     DASUFX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174189"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="VFV99_15570"
     CDS             <1..1241
                     /locus_tag="VFV99_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060787.1"
                     /translation="DRPTDLAGLSFELYVDGMFFAQTEGTFDGTIYIPDVPDGPFVLK
                     TSRATEQPHWEQREAHDFVVVAPRVGRPDAVLATSAPLHLQLDNLAPWSSDDWLVADC
                     FENGTEHYPLTLDARLAVGATQVDASFDWADAAWGPYLLDGAAGDRVTISHYVNDPRT
                     DLDVTRLTQVATGSAPSQTDGAPSDFAAAFVDVAATLSLTVHVDLAALAAAVPQTNPN
                     WGVTLLKGPGSHDLDWFGPQLVSIGAVNPPMDGQVVAATTYGDPFNRDWPLLVVAGYG
                     AFELLRAPDGSGYSARHYAYDSRVYDGSEYTLEIQEPVTSGTLNDYPLIGDIPWDGKS
                     PVTLQIDVPPGRNGFSALLNLAQSNRGLIRSDRNEITLPSEAVRMGVDYFLDVHVSTE
                     TETSSSGVFSTLGPFRLVPP"
     gene            complement(1305..1712)
                     /locus_tag="VFV99_15575"
     CDS             complement(1305..1712)
                     /locus_tag="VFV99_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060788.1"
                     /translation="MRYLVLLALLTTTAHADDAPLPATGKMVDKVVEMTNQFFDQHVD
                     LLTHDMLYVRVDAEHRAAKIAVGGGDAHLMKLRFAGNVEVVDGTARIHSRLALGLGGK
                     QLDLRLPNIDVSAASYRGERGVEVRLPLLRRSF"
     gene            1823..2380
                     /gene="msrB"
                     /locus_tag="VFV99_15580"
     CDS             1823..2380
                     /gene="msrB"
                     /locus_tag="VFV99_15580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5060789.1"
                     /translation="MIRALALAMLFAACNANSSSAAPAAETKADVKTHTMKHPIRTTK
                     PSDDVIRKELTPLQYEVTQRDGTEPPFHNEYWDNHEAGIYVDIVTGEPLFSSTNKFDS
                     GTGWPSFYKPIGNIVEKRDVSHSMARVEVRSKIGDSHLGHVFDDGPKPTGLRYCIDSA
                     ALRFIPADQLEAQGYAQFTSLFVKR"
     gene            complement(2360..>5049)
                     /locus_tag="VFV99_15585"
     CDS             complement(2360..>5049)
                     /locus_tag="VFV99_15585"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060790.1"
                     /translation="HVLRELGRGSMGLVLRAYDPELARPVAIKLLRDVKRDELRDEAR
                     TLARLRHPNVVTVYDVFTDDHGVYLAMELVDGDTLRGYCKGRSQREVIAACVRAGRGL
                     AAAHDAGVIHRDFKPENVLCGGDADVRVSDFGLAHTTDAAPDGTVAGTPAYMAPEILR
                     REPATAASDQYSFCVTVHEMLTGTRPGEVDTSLPAWIQRVLRRGLARDPAGRYPSMHE
                     LLTALADDPKVRRRRRLLFGGVIGAALATGAVAVRLAQSSAPSCDIDDGALGDAWNAQ
                     RKQEVTRALGDRVARAIDIYAKSWVAVRHEACVATYDRGEQSRQALERRVACLDRGQH
                     QLGELTKLFTTVDPTLLPNALEAVYSLRDPYTCTGAESYTVPNDPASHVVVEEARTLL
                     DRARALQYGGKLDTADALAGQVVRLVEPLDISGLVAEAQLVQARVNIDRDNQGVAEDQ
                     LYKALHAAERAKDDRLVAETWIELVMTTGAQNHRFELAHSHAKAAEAAVARVNTPDLQ
                     VRLEYSLGALALAEAKLDDARTHLERAAALAGDDSRRLGQRGLIRSTLCDVERQAGKL
                     PAAKERCKQALEILEEAFGEDHMRVALTLNVIGGIAFAERDWATAERTYKRVIETLER
                     RKLTAQINYALALSNLGAVYSSKDDIKPAREYFTRALAVFEQHHPKHPQRLMPLQGLA
                     SLALRTGDTATAVERYTQVHDTMAASYAPEHPSLLVASYNLALAYVGNKMPDKAQAVL
                     DDLIARATTKGKESWMLAERAYDLSAQIADECKDYRSALALLQKAFAAVEHANDPTER
                     ALVLRHQGEIARHMNKPALAIAPLEQAIKDFGKDPDAYDIGATRYHLAFALWDSGRDK
                     KRAVEVAQQASDDLAKAQSGDALKQYRETLASFLALHKQ"
BASE COUNT          828 a         1720 c         1612 g          889 t
ORIGIN      
        1 tcgatagacc gaccgacctc gccgggctca gcttcgagct ctacgtcgac gggatgttct
       61 ttgcgcagac cgagggcacg ttcgacggaa cgatctatat cccggacgtt cctgacggtc
      121 cgttcgtcct aaagacaagc cgagcgaccg aacaaccaca ctgggagcag cgcgaggcgc
      181 atgacttcgt agtcgtcgcg ccgcgtgtag gacggccgga cgccgtgctg gcaacgagcg
      241 cgccgttgca tctccagctc gacaacctcg cgccatggag ctcggacgat tggctagtcg
      301 ccgattgttt cgagaacgga accgagcatt acccgctcac gttggatgcc cgcctcgcag
      361 tcggcgcgac acaggtcgac gcatcgttcg actgggcgga tgccgcgtgg ggaccatatc
      421 tactcgatgg cgcagcgggc gatcgcgtga ctatttcgca ctacgtcaac gatccgcgca
      481 cggacctcga tgttacgagg ctcacccagg tcgcaacggg ctcagcgccc tcgcagaccg
      541 atggagcgcc gagtgacttc gcggctgcgt tcgtcgatgt cgcggcgacg ctgtcactca
      601 ccgtccatgt ggatctcgct gcgctagcag cggcggtgcc acagaccaat cccaactggg
      661 gcgttacgtt actcaaggga ccggggtcgc atgacttgga ctggttcggt ccacagctcg
      721 tttcgatcgg tgcggtcaac ccgccgatgg acggccaggt cgtcgccgcc acgacatacg
      781 gtgacccgtt caatcgtgat tggccgctgc tcgtcgtcgc cggctatggt gcgttcgagc
      841 ttctgagggc ccctgacggc tcgggctatt cggctcgcca ttacgcgtac gactcgcgcg
      901 tctacgatgg cagcgagtac acgctcgaaa tccaagagcc agtcacatcg ggcacgctga
      961 acgactatcc actcatcggc gacatcccgt gggacggcaa atcgcccgtg acccttcaga
     1021 tcgacgtgcc gcctggacga aacggattct ccgcactgct caacctcgcc cagtcaaatc
     1081 gcggactgat ccgttcagac cgcaacgaga tcacgttgcc gagcgaggcg gtgcgcatgg
     1141 gtgtcgacta cttcctggac gttcatgtca gtaccgaaac cgagacctcc agctccggcg
     1201 tgttctcgac actcggcccg ttccgcctcg tgccgccgta gtggccatcg agcagtttgc
     1261 agcgacgacg ttcgtgctca gccacgaagt tcgcgctcac cgcgctagaa cgagcgccgc
     1321 aacagcggca gccgtacttc aacgccacgc tcgccgcgat aggaggcggc agagacgtcg
     1381 atgttcggca gtcgcaggtc tagctgcttg ccgccgagcc cgagcgcgag tcggctgtga
     1441 atgcgcgccg tgccgtccac gacctcgacg ttgccggcga accgcagctt catgaggtgg
     1501 gcgtcaccgc caccgaccgc gatcttcgcc gcacggtgct cggcatcgac ccggacgtac
     1561 agcatgtcgt gggtcagcag atcgacgtgc tgatcgaaga actgattcgt catctcgacg
     1621 accttgtcga ccatctttcc ggtcgcgggc agcggtgcat cgtcggcatg agcggttgtc
     1681 gtaagcaggg cgagcaggac caggtagcgc atgccctacc ctcgcgcgtg accgcgtgaa
     1741 ggcactgtag gtgcacggtg aagtgcgcgt gatcgggccg taaccaacgc gcgatcacgg
     1801 cgtaagcagg ccaggaggaa ccatgattcg cgccctcgct ctcgcaatgt tgttcgcggc
     1861 ttgcaacgcc aactcgtcgt cggccgcgcc cgcagccgag accaaggccg acgtgaaaac
     1921 ccacaccatg aaacatccca ttcgcaccac gaaaccgtct gatgacgtga tccgcaaaga
     1981 gctcactccg ctgcagtacg aagtcaccca acgggatggc accgagccac cgttccacaa
     2041 cgagtactgg gacaaccacg aggccggtat ctacgtcgac atcgtgaccg gcgagccgct
     2101 gttctcgtcg acgaacaagt tcgactccgg caccggctgg ccgagcttct acaagccgat
     2161 cggcaacatc gtcgagaagc gcgatgtctc gcacagcatg gcccgcgtcg aggttcgcag
     2221 caagatcggc gattcgcacc tcggccacgt gttcgacgat ggcccgaagc ccaccggcct
     2281 gcgttactgc atcgactcgg ccgcgctccg cttcatacct gcggaccagc tcgaggcgca
     2341 gggctacgct cagttcacgt cactgtttgt gaagcgctag gaacgacgcg agcgtctcgc
     2401 ggtactgctt gagcgcatcg ccgctctgcg ccttcgcgag atcatcgctc gcctgctgcg
     2461 cgacttcgac cgcgcgcttc ttgtcgcgtc cgctgtccca cagcgcaaat gcgaggtggt
     2521 agcgcgtggc gccgatgtcg tacgcatcgg ggtccttgcc gaagtctttg attgcctgct
     2581 cgagcggcgc gatcgcgagc gctggcttgt tcatgtggcg cgcgatctca ccctggtgac
     2641 gcagcacgag cgcacgctcg gtcggatcgt tcgcgtgctc gaccgcggca aacgcctttt
     2701 gcaggagcgc gagcgcgctg cgatagtctt tgcactcgtc ggcgatctgc gccgacagat
     2761 cgtaggcgcg ctcggcgagc atccagctct ccttaccctt ggtcgtcgcg cgcgcgatca
     2821 ggtcgtcgag cactgcttgc gccttgtcgg gcatcttgtt gcccacgtac gcgagcgcca
     2881 ggttgtagct ggcgacgaga agcgacgggt gctcgggcgc gtacgacgcc gccatcgtgt
     2941 cgtggacctg cgtgtagcgc tcgaccgcgg tggccgtatc gccggtgcgg agcgcgaggc
     3001 tcgcgagtcc ctgcagtggc atgagccgct gcgggtgctt tggatgatgt tgctcgaaga
     3061 ccgcgagcgc gcgcgtgaag tattcgcgcg ctggcttgat gtcgtccttc gacgaataca
     3121 ccgcaccgag gttcgatagc gcgagcgcgt agttgatctg cgccgtgagc ttgcgccgtt
     3181 ccagcgtttc gatcacgcgc ttgtacgtgc gctcggccgt tgcccagtcg cgctccgcga
     3241 acgcgattcc accgatcacg ttgagcgtga gcgcgacgcg catgtgatct tcgccgaacg
     3301 cttcctcgag gatctcgagt gcttgcttgc agcgctcctt cgcggccggc agcttgcccg
     3361 cctgccgctc gacatcgcac agcgtcgaac gaatcagacc gcgctgaccg agccggcgcg
     3421 agtcgtcgcc cgccagcgcc gccgcgcgtt cgagatgggt acgcgcatca tcgagctttg
     3481 cctcggcgag cgcgagcgcg ccgagcgagt actcgagccg cacctgcagg tcgggggtgt
     3541 tcacgcgcgc cacggccgct tccgccgcct tcgcgtgcga gtgcgcgagc tcgaaccggt
     3601 ggttctgggc gcccgtggtc atcacgagct cgatccacgt ctcggcgacg agccgatcat
     3661 ccttcgcgcg ttcggccgca tgcagcgcct tgtagagctg gtcctcggcc acaccttggt
     3721 tgtcgcgatc gatgttgacg cgcgcctgga cgagctgcgc ctcggcaacc aagccgctga
     3781 tgtcgagcgg ctcgaccaag cggacgacct gcccggccaa tgcatccgcc gtgtcgagct
     3841 tgccgccata ctgcaacgca cgcgcgcgat cgagtagcgt gcgcgcctct tcgacgacca
     3901 cgtgcgacgc cggatcgttc ggcaccgtgt acgactcggc gcctgtacac gtgtacggat
     3961 cgcgcagcga gtacacggct tcgagtgcat tgggcagcag tgtcgggtcg acggtcgtga
     4021 acagcttcgt gagctcgccg agctggtgct gcccgcgatc gaggcacgcg acccgccgct
     4081 cgagtgcttg ccgcgattgc tcgccgcggt cgtaggtcgc aacacacgcc tcgtggcgga
     4141 ccgccaccca tgacttcgcg tagatgtcga tcgcgcgcgc gacgcggtcg ccaagcgcac
     4201 gcgtcacctc ttgtttgcgt tgcgcgttcc acgcgtcgcc cagcgcgccg tcatcgatat
     4261 cgcagctcgg tgccgatgac tgcgccagcc tgaccgcgac cgcgccggtc gcgagcgccg
     4321 caccgatgac gccgccgaac agcagccgcc gacgccggcg caccttcgga tcgtcggcga
     4381 gcgcggtgag cagctcgtgc atcgacggat aacgtcccgc cggatcacgc gcgaggccgc
     4441 ggcgcagcac gcgctggatc cacgccggca acgacgtatc gacctcgccg gggcgcgtgc
     4501 ccgtcagcat ctcgtgcacg gtgacgcaga agctgtactg atcgctcgcg gcggtcgctg
     4561 gttcgcgccg caggatctcc ggcgccatgt acgcgggcgt gcctgccacc gtgccgtcgg
     4621 gcgccgcgtc ggtcgtgtga gcgagcccga agtccgacac gcgaacgtcc gcgtcgcctc
     4681 cgcacagcac gttctcgggt ttgaagtcgc ggtggatcac accggcatcg tgagcggccg
     4741 cgagtccgcg ccctgcccgc acgcacgccg cgatcacctc gcgctgcgaa cggcccttgc
     4801 agtaaccgcg cagcgtgtcg ccatcgacga gctccatcgc gaggtacacg ccgtggtcgt
     4861 cggtgaacac gtcgtacacg gtcacgacgt ttggatggcg caaccgcgcg agcgtgcgcg
     4921 cttcgtcgcg gagctcgtcg cgcttcacgt cgcgcagcag cttgatcgcg accggccgcg
     4981 caagctcggg gtcgtacgcg cgcagcacca gccccatcga gccgcggccc agctcgcgca
     5041 gcacgtgat
//
LOCUS       DASUFX010000390         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_340829,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000390 DASUFX010000000
VERSION     DASUFX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340829"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFV99_15590"
     CDS             <1..475
                     /locus_tag="VFV99_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5060791.1"
                     /translation="VSRVMLTAITEQSLTAIEPQAATRNVKVEVTIPDGLAVKADPKA
                     LDQILVNLIDNGVKYARADGHVWVEAREVGDTVRIEVRDDGPGIADKHRERVFERFYR
                     ADPSRTREAGGTGLGLSIVKHLVESMGGDVGVEPNAPRGSIFWLRLPRAAEQVTA"
     gene            472..1158
                     /gene="phoU"
                     /locus_tag="VFV99_15595"
     CDS             472..1158
                     /gene="phoU"
                     /locus_tag="VFV99_15595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5060792.1"
                     /translation="MIHSHTSKEFETELRNLRERLCQMGGKCERQIALAMQALTDRDD
                     EIARKVIDGDHAIDRDETEIDELALQILATRQPVASDLRFITMALKFVTDLERIGDHA
                     AGIAKRVLELNHVPPLDNAPDLRSFAQLVLKNLHAALDAFVARDADAAMGLIQADREV
                     DKRNASLFAELLAHVATDPASVTRVLPLTSVCRYLERIGDHVKNLAEEVIYMVRAEDL
                     RHRPLPKPKN"
     gene            1209..2738
                     /locus_tag="VFV99_15600"
     CDS             1209..2738
                     /locus_tag="VFV99_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX5060793.1"
                     /translation="MRSLLASVVPLVSLVATSCGGSKMYAAAPSEMVAAGAPQFYEPT
                     AANSEDYTNYGKNPWVSAADDRFSTFAADVDTASYTIARRKLQEGTLPPQASVRVEEW
                     VNYFKYSFPAAAQGTPFSVVMEAAPHPFEPSRHVLRVGVATKAKTVGERKPSALVFLV
                     DVSGSMSSDDKLGLAKKALHLLTDNLSEKDAVAIVTYAGADRLVLPMTSIDHKDRIHK
                     AIDSLTSGGSTAMAAGIDTAYDLAAKWVRPGGISRVIVLSDGDANVGPHSYQELLKII
                     EDRAKAGVALSTIGFGMGNYKDTTMEQLADKGNGNNYYIDSLDAAKRIFSEQLTSTLE
                     VAAKDVKLQVEFDPAQVEHYRLLGYENRDIKDEDFRKDSIAAGQVGWGHQVTALYELE
                     LTAAGKAHPAPLGTVRIRHKQPEADAATESTFAMAAPPASSLAAASSELRFAFAVAAF
                     ADVLRGGAEWSLDEIRGHAAAATGNDKDRTELLSLIDKARSLRGNTNHVATDAPSTIA
                     K"
     gene            2778..3017
                     /locus_tag="VFV99_15605"
     CDS             2778..3017
                     /locus_tag="VFV99_15605"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEX5060794.1"
                     /translation="MARTSTARKTPTNVSIRADLVKRAKELGLNLSGLLESALEEAIR
                     TAEREQWMRENEEAIDYWNEWVKKHGSFADQWRKF"
BASE COUNT          605 a         1028 c         1013 g          474 t
ORIGIN      
        1 ggtgtcgcgc gtgatgctca cggcgatcac ggaacagtcg ctgacggcga tcgagcccca
       61 ggcggcgacg cgcaacgtga aggtcgaggt cacgattccc gatgggttgg cggtgaaggc
      121 cgaccccaag gcgctcgatc agatcctcgt caatctcatc gacaacggcg tcaagtacgc
      181 gcgcgccgac ggccacgtgt gggtcgaggc gcgcgaggtc ggcgatacag tccgcatcga
      241 ggtgcgcgac gacggccccg gcatcgccga caagcaccgg gagcgggtgt tcgagcggtt
      301 ctaccgggcg gatccgagcc gaacgcgaga agccggcggc accggccttg gcctctcgat
      361 cgtcaagcat ctcgtcgaga gcatgggtgg cgacgtcggg gttgagccga acgcgccacg
      421 cggtagcatc ttctggttgc ggctgccgcg agcggccgag caggtgacgg catgatccac
      481 tcacacacca gcaaagagtt cgaaaccgag ctgcgcaacc tgcgcgagcg cctgtgccag
      541 atgggtggca agtgcgagcg ccagatcgcg ctcgcgatgc aggctctcac cgaccgcgac
      601 gacgagatcg cgcgcaaggt aatcgacggt gaccacgcga tcgatcgcga tgagaccgag
      661 atcgacgagc tcgcgctgca gatcctcgcg acccgacaac cggtcgcctc cgatctccgc
      721 ttcatcacga tggcgctcaa gttcgtgacc gatctcgagc gcatcggtga ccacgccgcc
      781 ggcatcgcga agcgcgtgct cgagctcaat cacgtgccgc cgctcgacaa cgcacccgat
      841 ctgcggtcgt tcgcgcagct cgtgctcaag aacctgcacg cggcactcga cgcattcgtc
      901 gcgcgcgacg ccgacgccgc gatgggattg atccaggcgg accgcgaagt cgacaagcgc
      961 aacgcgagcc tgttcgccga attgctcgcg cacgtcgcga cggatccggc gtcggtcacg
     1021 cgcgtgctcc cgctgacctc ggtgtgccgc tatctcgagc gcatcggcga ccacgtgaag
     1081 aacctcgccg aagaggtcat ctacatggtg cgcgctgaag acctccgtca ccggccgctg
     1141 ccaaagccga agaactagag cggaagttac gtgctgtaag tcgcagcgcc gcgtgtcgtt
     1201 ggatcggcat gcgctcactt ctcgcgtccg tcgtcccgct cgtatcgctc gtcgccacca
     1261 gttgtggcgg ttccaagatg tacgcagccg cgccaagcga gatggtggcc gcaggcgcgc
     1321 cccagttcta cgagcccacc gcggcgaaca gcgaggacta cacgaactac ggcaagaatc
     1381 cgtgggtctc cgccgccgac gatcgcttct cgacgttcgc cgcagatgtc gacaccgcgt
     1441 cgtacacgat cgcgcgccgc aagctgcagg aaggcacgct gccgccgcaa gcgtcggtgc
     1501 gcgtcgaaga gtgggtgaac tacttcaagt acagcttccc agcggccgcg cagggcacgc
     1561 cgttctcggt cgtgatggaa gcggcaccgc atccgttcga gccgagccgt cacgtgctgc
     1621 gcgtcggcgt cgcgacgaaa gcgaagaccg tgggcgagcg caagccgagc gcactcgtgt
     1681 ttctcgtcga cgtctcgggc tcgatgagct cggatgacaa gctcggcctc gcgaagaagg
     1741 cgctgcacct gctcacggac aacctcagcg aaaaggacgc cgtcgcgatc gtcacttacg
     1801 cgggcgcgga ccgcctcgtg ctgccgatga cgagcatcga ccacaaagat cgcatccaca
     1861 aagcgatcga ttcactcacg tcgggcggct cgaccgcgat ggccgcgggc atcgataccg
     1921 cgtacgacct cgccgcgaag tgggtccgcc ccggcggcat ctcgcgcgtg atcgtgctct
     1981 ccgacggtga cgcgaacgtt ggcccgcact cgtatcagga gctgctcaag atcatcgagg
     2041 accgcgcgaa agccggtgtc gcgttgtcga cgatcggctt cggcatgggc aactacaaag
     2101 acacgacgat ggagcaactc gccgacaagg gtaacggcaa caactactac atcgactcgc
     2161 tcgacgcggc gaagcgcatc ttcagcgagc agctcacgtc gacactcgag gtcgccgcca
     2221 aggacgtcaa gctgcaggtc gagttcgatc ccgcgcaggt cgagcactat cgcctgcttg
     2281 gctacgagaa ccgcgacatc aaggatgaag acttccgcaa ggacagcatc gcggccggtc
     2341 aggtcggctg gggccatcag gtcaccgcgc tgtacgagct cgagctcacc gcggctggca
     2401 aggcgcaccc ggcgccactc ggtaccgttc gcatccgcca caagcagccc gaggctgacg
     2461 cagcgaccga gtcgacgttc gcgatggccg cgccgccggc gtcgtcgctc gcggctgcct
     2521 cgtcggagct gcgctttgcg ttcgcagtgg cagcgttcgc cgacgtgctt cgtggtggtg
     2581 cggagtggtc gctcgacgag atccgcgggc acgcggctgc ggcgacaggc aacgacaagg
     2641 accgcaccga gctgctgtcg ctgatcgaca aggcccgctc gctgcgtggc aacacgaacc
     2701 acgtcgcgac cgacgcgccg tcgacgatcg cgaagtagcc ttgccggggc agattgatgc
     2761 gcatatagta tgcgcatatg gcgcgaacat cgacagcgcg gaaaacgcct acgaacgtgt
     2821 cgattcgcgc cgacctcgtg aaacgcgcga aggagctcgg cctgaacctc tcgggactgc
     2881 tcgagagcgc tctcgaagaa gcgattcgga ccgccgagcg cgaacagtgg atgcgagaga
     2941 acgaggaagc gatcgactac tggaacgaat gggtcaagaa gcacggcagc ttcgccgacc
     3001 aatggcggaa gttctaagtt ggcgcagttc gacgtccaca agaatccgcg cggcggtccc
     3061 taccccctgc tgctcgacat tcagtcgaac gtgttggccc gaaccgaaca tcgagtcgtc
//
LOCUS       DASUFX010000391         7626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_202381,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000391 DASUFX010000000
VERSION     DASUFX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202381"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFV99_15610"
     CDS             complement(<1..214)
                     /locus_tag="VFV99_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060795.1"
                     /translation="MLLVDAGQTHDVFDVVEVTDALLRVRTAFLFDIGEEMKVRIEQN
                     GAVFEAMARVLGHSGTADDRITELELA"
     gene            322..1059
                     /locus_tag="VFV99_15615"
     CDS             322..1059
                     /locus_tag="VFV99_15615"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5060796.1"
                     /translation="MLKPVEKQRVAEEIAEQLRGLILNGQYPPGSKLPPERELSKRLR
                     VNRASLREALKKLEHLGLVRIRQGDGTRVQNFMETGGIELVQHLLPLGGGKPELIRDL
                     LEFRRIFGRELARLAASRSSKDKDGLTKLRAVADKADHTQGAAELFDLDFDFYVAIAS
                     LSGNQVMLLLINTVRDGVRNFMPLLANLAAPSDAVRKHHRELIAAIEKGDVPGAGRIA
                     DEYLRMGAEMAARVGGRPELQPQPLPE"
     gene            1063..1362
                     /gene="gatC"
                     /locus_tag="VFV99_15620"
     CDS             1063..1362
                     /gene="gatC"
                     /locus_tag="VFV99_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5060797.1"
                     /translation="MPALTRKEVEEIALLARLHLDASELDQMQGELGAILEHFAALAQ
                     VDTNDVQPMTHAVPMDLRLRPDEVAPSLSPEDALRGAPVKQDSVFVVPAIIGGNE"
     gene            1359..2819
                     /gene="gatA"
                     /locus_tag="VFV99_15625"
     CDS             1359..2819
                     /gene="gatA"
                     /locus_tag="VFV99_15625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5060798.1"
                     /translation="MSEWLSVADTAKKIAAGELTAVRVVDTYLSRIMNHDRELGSYLA
                     VDGEGAREAAKAIDAQRAEGKRLGPLGGVPISLKDVLVTRGLETTAASKILEGWIPPY
                     DGTVVEKLRAAGAIVLGKVNCDEFAMGSSTENSAYKRTRNPWDTQRVPGGSSGGSSAS
                     VAAGLCAASLGTDTGGSIRQPAAFCGVVGLKPTYGRVSRWGVVAFASSLDQVGPLTRS
                     VHDAALVLEVIAGFDPRDSTSLDVPVPPYTDALVGDVTGLKVGLPREYFEGEIDNEVK
                     AALDKTIAALKDRGAQIVDISLPHTSLALAAYYLVAPAEASSNLARYDGIRFGKAAQG
                     AKDLLDLYMKSRGAGFGREVKRRIMLGTYALRSGYYDAYYKKAQQVRALIKRDFDDAF
                     GSVDVILTPTTPTPAFRFGAKATPMDMYLGDVFTLSCNLAGLPGISVPCGLTSDGLPI
                     GAQLLGKPLDEATVLRVGHVIESLVGLRDQRPKAVS"
     gene            2816..4273
                     /gene="gatB"
                     /locus_tag="VFV99_15630"
     CDS             2816..4273
                     /gene="gatB"
                     /locus_tag="VFV99_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5060799.1"
                     /translation="MTELAQLLETWEPVIGLEVHVQLNTKSKLFSPSAVQFGAPPNSL
                     TDPLVLGMPGTLPVFNRQALELALRLGVATESKIRAKSRFARKQYFYPDLPKGYQISQ
                     YDEPLCEDGRLDIIVGGAVKRIKLERIHIEEDAGKNTHVGQFSHVDLNRAGVPLVEVV
                     TAPELTSAEEAAEFMRSLHRLVRWLEISEGDMEKGQMRCDANVSVRIRGEERLGTRTE
                     LKNINSFRFVQNAVEHEIARQVRIVDGGGRITRETRLWDADLGSSATMRSKEEANDYR
                     YFPDPDLPPLVVDGGMMGTILRTLPELPAARFTRYIEQHGVSPDDARTLISDRALAEY
                     FDVTCKLHPGERSGRTIANWMLTELLGALNAEGKDISQSPMQPATLSELIRLVEDGTI
                     SGKIGKEIFEESYKTGEAPTKIVERRGVQQISDEAALWEIVDRVVTANPKQATEYANG
                     KDGLFGFFVGQVMKQTQGRANPVVTSDLLHKRLGR"
     gene            4300..5136
                     /locus_tag="VFV99_15635"
     CDS             4300..5136
                     /locus_tag="VFV99_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060800.1"
                     /translation="MGWALLLLCGCNQVFGLRETIANDAQLFDAPIDAPFTCPDPAMQ
                     PQFSKILHQVITKNCQAYMTSPESNRAVAYCVDMDVVVDGVIDAEPQPATLTPVGQYD
                     WPRLTPEGDEIWVRDRRVPPTFAVFQWQSDHTWAHVRDLVIANVARDDYITAPSRKVG
                     GKRRFVRLAFSEFKLYEYDDDGTTTTPIRSYNLQLDLGVDFASFPNLDASGLRLVFVG
                     QTTGTSFTQTFYASRASIDDSFSPAKPLLAAPLAYDPFLTPDCSRLYTSGLGSIFYAQ
                     QL"
     gene            complement(5139..6026)
                     /locus_tag="VFV99_15640"
     CDS             complement(5139..6026)
                     /locus_tag="VFV99_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060801.1"
                     /translation="MERRPPRGTDNNGLLLGNLADGTRVGEFVIEARLGARGTGHLYA
                     ATHLVLPRRATIHVLPPAEGPARTVALELLRAACIVDAIDHPGMPRVFECGMFPDHRP
                     WIASERIEGQTVATMLDSRRVSLGDVIAIVRDVAAVIAEAHRRGLVHGHITPAAIVFT
                     PRGRRFPICLVDWVGARTHDSTAPLPLVVGSPYVAPEQANGIRIDDRSDVYSLGRIGS
                     ALLDCVTGDEQSPMLVALLDSMTADDRDVRPASEYVEQTAAWLAGQLPAQPVAVAAER
                     SAPITSEVAIAVAGEIAPR"
     gene            6192..6509
                     /gene="rplU"
                     /locus_tag="VFV99_15645"
     CDS             6192..6509
                     /gene="rplU"
                     /locus_tag="VFV99_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5060802.1"
                     /translation="MYAVIQTGGKQYRVEPGKTITVEKLAGDAGAQVVFDQVLLVSSG
                     DGGNVTVGKPLVAGAKVTASIVEQTRGDKLVVFKFRRRKNYVRRNGHRQDMTVVKVAE
                     IKA"
     gene            6522..6788
                     /gene="rpmA"
                     /locus_tag="VFV99_15650"
     CDS             6522..6788
                     /gene="rpmA"
                     /locus_tag="VFV99_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5060803.1"
                     /translation="MAHKKGQGSTRNGRDSNPKMRGVKRFAGEQVTAGSILVRQVGTK
                     FHAGENVGIGRDWTLFAKITGVVQFERHGKSKMRVAVYPQAAAA"
     gene            complement(6823..7047)
                     /locus_tag="VFV99_15655"
     CDS             complement(6823..7047)
                     /locus_tag="VFV99_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060804.1"
                     /translation="MKRFIALILLSTALAVGTTACAHQQLTKLHAAETVATAAVIAGM
                     VLLAAQAKCGDCNVGIEDPNTSARPAQPPR"
     gene            complement(7044..7247)
                     /locus_tag="VFV99_15660"
     CDS             complement(7044..7247)
                     /locus_tag="VFV99_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060805.1"
                     /translation="MKRLIAFVSLSAALATTGCAHKQLTNEQVAKYAVTGASAVGLVV
                     LFIASGVAKQNAGPTSANLGAQP"
     gene            complement(7244..7462)
                     /locus_tag="VFV99_15665"
     CDS             complement(7244..7462)
                     /locus_tag="VFV99_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060806.1"
                     /translation="MKSLAIALAAALGLPGCAHKPVSNDNLARSAVTVGAAVLITSAL
                     VYAEYHSQSAGDVGGATAAALPPLGAHQ"
BASE COUNT         1304 a         2499 c         2569 g         1254 t
ORIGIN      
        1 cggccagctc gagctcggtg atcctgtcgt cggcggtccc gctgtggcct aggacccggg
       61 ccatcgcctc gaaaaccgcc ccgttctgct cgattcggac cttcatttcc tccccgatgt
      121 cgaagagaaa agcggtcctc accctcagca gcgcgtcggt gacctcgacg acgtcgaaga
      181 cgtcgtgggt ctgcccggcg tcgacgagca gcaccgatgc gctcgtggca ggctccggca
      241 tccgagcgga ggtaccacgc tacgcgcggg cgcctcaagc tcttctacta ggcggcgcgc
      301 cgggttaacc tcattcggct gatgctcaag cctgtcgaga agcagcgtgt cgccgaggag
      361 atcgccgaac agctgcgtgg cctcatcttg aatggccaat atccgccggg ctcgaagctg
      421 ccgccggaac gcgagctctc gaagcgattg cgcgtgaatc gcgcgtcgct ccgcgaggcg
      481 ctcaagaagc tcgagcatct cggcctcgtg cgcatccgcc agggcgatgg cactcgcgtt
      541 cagaacttca tggagaccgg cggcatcgag ttggtccagc atttgttgcc gctcggcggt
      601 ggcaagcccg agctgattcg cgacctgctc gagttccgac gcatcttcgg acgcgagctc
      661 gcgcggctcg cagcgtcgcg tagctccaag gacaaggacg ggctgaccaa gcttcgtgcc
      721 gtcgccgaca aggctgatca cacgcagggc gcggccgagc tgttcgatct cgacttcgat
      781 ttctacgtcg cgatcgcgtc actgtcgggc aatcaggtca tgctgctgct catcaacacc
      841 gtgcgcgatg gcgtccgcaa tttcatgcct ctgctcgcga acctcgccgc gccgtcggat
      901 gccgtgcgca agcatcaccg cgagctcatc gcggcgatcg agaagggtga cgttccgggc
      961 gccggccgca tcgccgacga gtacttgcgg atgggggccg agatggctgc gcgcgtgggc
     1021 ggccgtcccg agctccagcc tcaacctttg cccgagtagc tcatgccggc tctgacgcgg
     1081 aaggaggtgg aggagatcgc gctcttggcg agactccacc tcgacgcgag cgagctcgat
     1141 caaatgcagg gcgagctcgg cgcgatcctc gagcacttcg ccgcgctcgc acaggtcgac
     1201 accaacgacg tgcaaccgat gacgcacgcg gtgccgatgg atctccggct gcgtcccgac
     1261 gaggtcgcgc cgtcgctgtc gcccgaggat gcgctgcgcg gcgcacccgt gaagcaggac
     1321 agcgtgttcg tcgtccccgc gatcatcggg ggcaacgagt gagcgagtgg ttgtcggttg
     1381 ccgacaccgc gaagaagatc gcggccggcg agctcaccgc ggtgcgtgtc gtcgacacgt
     1441 acctgtcgcg gatcatgaac cacgatcgcg agctcggctc gtatctcgcg gtggacggcg
     1501 aaggcgcgcg cgaggctgca aaggcgatcg acgcgcaacg cgcggagggc aagcggctcg
     1561 gcccgctcgg cggcgtcccg atctccctca aggacgtgct cgtcacccgc gggctggaga
     1621 ccaccgcagc gtcgaagatc ctcgagggct ggatcccgcc gtacgacggc accgtcgtcg
     1681 agaagctgcg cgctgccggc gcgatcgtcc tcggcaaggt gaactgcgac gagttcgcga
     1741 tgggcagctc gaccgagaac tccgcgtaca agcgcacgcg caatccgtgg gacacccagc
     1801 gcgtgccggg cggcagctcg ggcggcagct cggcgtcggt cgccgcgggt ctgtgtgcgg
     1861 cgagccttgg caccgatacc ggcggttcga ttcgccagcc ggcggcgttc tgtggtgtcg
     1921 tcgggctcaa gccgacgtac gggcgtgtat cgcgctgggg cgttgtcgcg ttcgcgtcgt
     1981 cgctcgatca ggtcggccca ctcacgcgct cggtgcacga tgccgcgctc gtgctcgaag
     2041 tgatcgcggg tttcgatccg cgcgactcga cgtcgctcga cgtgccggtg ccgccgtaca
     2101 ccgacgcgct cgtcggtgac gtcaccgggc tcaaggtcgg cctgccgcgc gagtacttcg
     2161 agggcgagat cgacaacgag gtgaaagccg cgctcgacaa gacgatcgct gcgctcaagg
     2221 accgcggcgc gcagatcgtc gacatctcgc tgccgcacac gtcgctcgcg ctcgccgcgt
     2281 attacctcgt cgcgcccgcc gaagcgtcat cgaacctcgc gcgctacgac ggcatccgct
     2341 tcggcaaggc agcgcagggc gccaaggacc tgctcgacct ctacatgaag tcgcgcggcg
     2401 ccggctttgg ccgcgaggtc aagcgccgca tcatgctcgg cacctacgcg ctgcgctccg
     2461 ggtactacga cgcgtactac aagaaggccc aacaagtccg cgcgctgatc aagcgcgact
     2521 tcgacgacgc attcggaagc gtcgacgtca tcctcacgcc gacgacgccg acccctgcgt
     2581 tccgcttcgg tgcgaaggcg acgccgatgg acatgtacct cggcgacgtg ttcacgctgt
     2641 cgtgcaacct ggcggggctg ccgggcatca gcgtcccgtg cggcctcacg agcgacggcc
     2701 tgccgatcgg cgcgcagctg ctcggcaagc cgctcgacga agcgaccgtg cttcgcgtcg
     2761 gtcacgtgat cgaatcgctc gtcggtctgc gcgatcagcg gccgaaggcg gtgtcgtgac
     2821 cgagctcgcg caattgctcg agacgtggga gccggtgatc ggtctcgagg tccacgtcca
     2881 gctgaacacg aagagcaagt tgttctcgcc ctcggcggtg cagttcggcg cgccgccgaa
     2941 ctcgctcacc gatccgctcg tgctcggcat gcccggcacg ctgcccgtgt tcaaccgtca
     3001 agcactcgag ctcgcgctgc ggctcggcgt cgcgaccgag tcgaagatcc gcgcgaagtc
     3061 gcggttcgcg cgcaagcagt acttctatcc ggatctgccg aagggctatc agatctctca
     3121 gtacgacgag ccgctgtgcg aggacggtcg cctcgacatc atcgtcggtg gcgcagtcaa
     3181 gcggatcaag ctcgagcgca tccacatcga agaggatgcc ggcaagaaca cgcacgtcgg
     3241 tcagttctcg cacgtcgatc tcaaccgcgc gggtgtgccg ctcgtcgagg tcgtgacggc
     3301 acccgagctg acgagcgccg aggaggccgc cgagttcatg cgctcactgc atcggctcgt
     3361 gcgctggctc gagatctccg agggcgacat ggaaaagggg cagatgcgct gcgacgcgaa
     3421 cgtgtcggtg cgcatccgtg gcgaggagcg gctcggtacg cggaccgagc tcaagaacat
     3481 caactcgttt cgtttcgtgc agaacgccgt cgagcacgag atcgcgcgcc aggttcgcat
     3541 cgtcgacggc ggcggacgca tcacccgcga gacgcggcta tgggatgccg acctcggctc
     3601 gtcggcgacg atgcgctcga aagaggaagc gaacgactat cgttacttcc cagatcccga
     3661 tctgccgccg ctcgttgtcg atggcggcat gatgggcacg atcctccgca cgctgcccga
     3721 gctaccggca gcgcgcttca cgcgctacat cgagcagcac ggcgtttcgc cggacgacgc
     3781 acgcacgctg atcagcgatc gcgcgctcgc cgagtacttc gatgtcacgt gcaagctgca
     3841 cccgggcgaa cgctcgggcc gcacgatcgc gaactggatg ctcaccgagc tgctcggcgc
     3901 gctcaacgcc gaaggcaaag acatctcgca atcgccgatg cagccggcga cgctgtcgga
     3961 gctgatccgg ctcgtcgagg acggcacgat cagcggcaag atcggcaaag agatcttcga
     4021 ggagagctac aagaccggcg aggcgccgac caagatcgtc gagcgccgcg gcgtgcagca
     4081 gatctccgac gaggcggcgc tgtgggagat cgtggaccgt gtggtcaccg cgaacccgaa
     4141 gcaggcgacc gagtatgcaa atggcaagga cggtctgttc ggattcttcg tcggccaggt
     4201 gatgaaacag acccagggcc gcgcgaatcc ggtcgtcaca tccgacctgt tgcacaagcg
     4261 tctgggccgc tgacgtacta aagtacagaa gtgcgcgcga tggggtgggc gctgctgctc
     4321 ttgtgcggct gcaatcaggt ctttggcctg cgtgagacga tcgcgaacga cgcgcagctg
     4381 ttcgacgcgc cgatcgatgc gccgttcacg tgccccgatc ccgcgatgca gccgcagttc
     4441 tccaagatcc tgcatcaggt gatcacgaag aactgccagg cgtacatgac gagccccgag
     4501 agcaatcgcg cggtcgccta ctgtgtcgac atggacgtag tcgtcgacgg tgtcatcgat
     4561 gccgagccac agccggccac gctcacaccg gtaggccagt acgactggcc gcggctgacg
     4621 ccggaagggg acgagatctg ggtgcgcgat cgccgcgtgc cgccgacgtt cgccgtattt
     4681 cagtggcaga gcgatcacac ctgggcgcac gtgcgcgatc tcgtgatcgc gaacgtcgcg
     4741 cgcgacgact acatcacggc gccgtcgcga aaggtcggtg gcaagcgccg gttcgtgcgg
     4801 ctcgcgttca gcgagttcaa gctctacgag tacgacgacg acggcaccac cacgacaccg
     4861 attcgctcgt acaacctgca gctcgatctc ggcgtcgact tcgcgagctt tccgaacctc
     4921 gatgcgagtg ggttgcggct cgtgttcgtc ggccagacaa ccggcacgag cttcacgcag
     4981 acgttctacg ccagtcgcgc gagcatcgac gattcgttct cgccagcgaa gccgctgctc
     5041 gccgcgccgc ttgcctacga tccattcctc acgccggact gcagccggct gtacacgagc
     5101 ggcctcggtt cgatcttcta cgcgcagcag ctctgagctc agcgcggcgc gatctcgccg
     5161 gcgaccgcaa tcgcgacctc ggacgtgatc ggcgcgctgc gctcggcggc gaccgccacg
     5221 ggttgcgcgg gcagctgtcc ggcgagccac gcagcggttt gctcgacgta ctcgctcgcc
     5281 gggcggacgt cgcgatcgtc agcggtcatc gaatcgagga gcgcgacgag catcggcgat
     5341 tgttcgtcgc cggtcacgca atcgagcagt gcgctgccga tgcggccgag cgagtagacg
     5401 tcgctgcgat cgtcgatgcg gataccgttt gcttgctccg gcgcgacgta cggactgccg
     5461 acgacgagcg gcagcggcgc ggtcgaatcg tgcgtgcgtg caccgaccca atcgacgagg
     5521 cagatcggga accggcggcc gcgcggcgtg aacacgatcg cggccggtgt gatgtggcca
     5581 tggacgagcc cgcggcggtg cgcttcggca atcacggcag caacgtcgcg cacgatcgcg
     5641 atgacatcgc cgagcgagac gcgccgactg tcgagcatgg tcgcgacggt ctggccctcg
     5701 atccgctccg acgcgatcca cggccgatga tcggggaaca ttccgcactc gaacacgcgc
     5761 ggcatgccgg ggtgatcgat cgcgtcgacg atgcacgcag cgcgaagcag ctcgagtgcg
     5821 acggtgcgcg ccgggccttc cgccggcggc agcacgtgga tcgtcgcccg gcgcggcaac
     5881 accaagtgcg tcgctgcgta gaggtgaccg gtgccgcgag cgccaagccg cgcctcgatc
     5941 acgaactcgc cgacgcgcgt gccgtccgcg aggttgccga gcagcaaccc gttgttgtcg
     6001 gtcccacgtg ggggccggcg ttccatcctg caggcataat gcatcccggc cgccctgggt
     6061 aggcttttgt cggatggagc caaccccttg atcatacgga tgatcgcggc tcaccccacg
     6121 agcaggcttg acgaaggccg cgcgatcagt aaagtccgcg attccgaaag cattttccgc
     6181 ggagccaagt catgtacgcc gtgatccaaa cgggtggcaa acagtatcga gtcgagccgg
     6241 gcaagaccat tacggtcgag aagctcgccg gcgatgcagg cgcccaggtc gtgttcgatc
     6301 aagttcttct cgtttcgtcg ggtgacggcg gcaacgtgac ggttggaaag ccgctcgttg
     6361 ccggcgcgaa agtcaccgct tcgatcgtcg agcaaacccg cggcgacaag ctcgtcgttt
     6421 tcaagttccg tcgccgcaag aactacgtgc gccgcaacgg tcatcgtcaa gacatgaccg
     6481 tcgtcaaggt cgccgagatt aaggcctaga ggtaacgcgt catggcacac aaaaaaggac
     6541 aaggctcgac tcggaacggt cgcgactcca acccgaagat gcgcggtgtg aagcgcttcg
     6601 cgggtgagca ggtcacggcc ggcagcatcc tcgttcgtca ggtcggcacg aagttccacg
     6661 cgggtgagaa cgtcggcatc ggtcgcgact ggacgctgtt tgcgaagatc accggcgtcg
     6721 ttcagttcga gcgccacggc aagtcgaaga tgcgcgttgc ggtgtatccg caagccgccg
     6781 ctgcctgatt cgcgagatgt gattttcgcg cgcggcttcg cgtcaacgcg gaggctgcgc
     6841 cggacgcgct gacgtattgg gatcttcgat cccgacgttg cagtcgccgc atttcgcctg
     6901 tgcggcgagc agcaccatgc cggcgatcac cgccgcggtg gcgacggtct cggcggcatg
     6961 cagcttcgtg agctgctgat gcgcgcacgc tgtggtgccg acggcgagcg cggtgctgag
     7021 caagatgagt gcgatgaagc gcttcatggc tgagctccta gatttgcaga cgtgggccca
     7081 gcgttctgct tcgcgacacc gctggcgatg aacaagacga cgagcccgac cgcactggcg
     7141 cccgtgacgg cgtacttcgc gacttgctcg ttcgtgagct gcttgtgcgc gcagcccgtg
     7201 gtggcgagcg cagcgctgag cgagacgaat gcgatgaggc gcttcattgg tgcgctccta
     7261 gcggcggcag cgcagcggcg gtcgcgccgc caacgtcgcc cgccgactgc gagtggtact
     7321 cggcgtagac gagcgcgctg gtgatcagta cagcagcgcc gaccgtcact gcgctgcgcg
     7381 cgaggttgtc gttgctgacc ggtttgtgtg cgcagccggg gaggccgagc gcggcggcga
     7441 gggcgatggc gagcgacttc atgaacccag cgtgcccgcc gtgcgcgtga agccactagg
     7501 cgtgccgcgt gagctacacg tgatcccaag gtcgtttcgg cgtcgcccta ggtcgcgccg
     7561 gcgagcgcaa ctacgcgcgc acccaggtgt tccagcaggc cggcgtcgtg gctggcgagg
     7621 atcgtc
//
LOCUS       DASUFX010000392         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1868529, whole genome shotgun sequence.
ACCESSION   DASUFX010000392 DASUFX010000000
VERSION     DASUFX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868529"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VFV99_15670"
     CDS             complement(<1..1276)
                     /locus_tag="VFV99_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5060807.1"
                     /translation="MAEPTRYTTLDRRPAVQIGRALASPRGVLVTLPLLVAAVGIGIM
                     LVGRDATSSATRSMARHQLAEQAKSVQTDTAFALDQAEPLMKLVRVVAERERPIDDVL
                     VRLHDMMAGRPGVAFLSISFPDGTFRGAQLMPDNHIEVQESTVDAAQAHWFVVENGAL
                     RPVKTEPSQYDPRTRGFYQLAVKTNARAWTEPYTFFKSHETGITCAEPVYNADGTLRA
                     VLTVDFHVGALSSFVARPAVDQARSVVYTQDGTILAYPAADHLDTPGDALLKVANVHD
                     PALTALFAAHRAGDSLRFVELSASDGNYLASVAPIGGSRAGVPAPLDWYVATLVPERT
                     LLGPTKKLMRSSIIASIGALGIAIGLALVLAWNLVRMRRQVQISREEARSAEARARDL
                     GSYRLVAKLGSGGMGEVWRAEHRLLARQAAIKL"
     gene            complement(1269..2021)
                     /locus_tag="VFV99_15675"
     CDS             complement(1269..2021)
                     /locus_tag="VFV99_15675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5060808.1"
                     /translation="MPGRTFAIGDIHGDLASLAKLFERLPTLAADDTVVFLGDYIDRG
                     PNSAGVVAWVRELTRTTPAKVVCLRGNHEDAWLQVLAEGWPEFVMPRGNGCLECFRSF
                     MGLPVPKEDEIPTYEEYEAMFDGRFFPADVADWMKTLPFFYEDEHAIYVHAGIKREGQ
                     IFPHPSEVEPKRALLWLRDHDFFEHYRGKLVVFGHTTTRTLPNELSTYTPEDPTDLWA
                     GPACVGLDTACGKGGFLTAFELPAKRVYESRG"
     gene            2143..>2350
                     /locus_tag="VFV99_15680"
     CDS             2143..>2350
                     /locus_tag="VFV99_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060809.1"
                     /translation="MKLPLVLASLFVAATAYAQAPGDYEEGTGAPGMSDPVAPTPPAP
                     PLRNQRWSIGLGVGSMSLAPHNAPD"
BASE COUNT          390 a          769 c          788 g          403 t
ORIGIN      
        1 tcagcttgat cgctgcctgc cgggcgagca gccgatgctc ggcgcgccac acttcaccca
       61 taccgccgga gccgagcttc gcgacgagcc ggtagctgcc gaggtcgcgt gcacgtgctt
      121 cggcgctacg cgcttcttcg cgcgagatct ggacctgccg ccgcattcgc acgaggttcc
      181 atgccagcac gagcgcgagc ccgatcgcga tgccgagtgc accgatgctc gcgatgatgc
      241 tcgaccgcat gagcttcttc gtcgggccga gcagcgtgcg ctcggggacg agcgtcgcga
      301 cgtaccagtc gagtggcgcc ggcacgcccg cgcgcgagcc accgatcggc gcgaccgacg
      361 cgaggtagtt gccgtcgctc gccgacagct cgacgaaccg caacgagtca ccagcgcgat
      421 gggccgcgaa cagcgcggtc aacgccgggt catgaacgtt ggcgactttg agcagcgcgt
      481 cgcctggcgt gtcgagatga tcggcggcgg gatacgcgag gatcgttccg tcctgcgtgt
      541 agacgaccga gcgcgcctga tcgaccgcgg gccgcgcgac gaagctcgac agcgcgccga
      601 cgtgaaagtc gaccgtgagc accgcgcgca gcgtgccgtc cgcgttgtag acgggttcgg
      661 cgcacgtgat gcccgtttcg tggctcttga agaacgtgta tggctccgtc cacgcgcgcg
      721 cgttcgtctt caccgcgagc tgatagaagc cgcgcgtgcg cgggtcgtac tgcgacggct
      781 cggtcttcac cgggcgcaac gcgccgttct cgacgacaaa ccagtgcgcc tgcgcagcat
      841 ctacagtgct ctcctgcacc tcgatgtggt tgtccggcat caactgcgcg ccgcgaaacg
      901 tgccgtcggg gaagctgata ctgagaaacg cgacgccggg tcggccggcc atcatgtcgt
      961 gcaggcgcac gagcacgtcg tcgatcggtc gctcgcgctc ggcgacgacg cgcacgagct
     1021 tcatcagcgg ctcggcttga tcgagcgcga atgcagtgtc ggtctgcacg ctcttcgctt
     1081 gttcggcaag ttgatgccgc gccatcgagc gcgttgcgct actcgtcgcg tcgcgaccga
     1141 cgagcatgat gccgataccg acggcagcga cgagcagcgg cagtgtgacg agcacaccgc
     1201 gcggtgacgc gagcgcgcgt ccgatctgga ctgcgggcct gcgatcgagc gtcgtgtatc
     1261 gagtcggttc agccacgcga ttcgtagacg cgcttggcag ggagctcgaa agcggtcaga
     1321 aagccgccct tgccacacgc ggtgtccaga ccgacgcacg cggggcctgc ccagaggtcc
     1381 gtcggatctt cgggggtgta cgtcgatagc tcgttcggca gcgtgcgcgt cgtcgtgtga
     1441 ccgaacacga cgagcttgcc gcgatagtgc tcgaagaagt cgtggtcgcg cagccagagc
     1501 agcgcgcgtt tcggctcgac ctccgacgga tgcggaaaga tctggccttc gcgtttgata
     1561 cccgcgtgca cgtagatcgc gtgctcgtcc tcgtagaaga acggcagcgt cttcatccag
     1621 tcggcgacgt cggcgggaaa gaaccggccg tcgaacatcg cttcgtactc ttcgtaggtc
     1681 ggaatctcgt cttccttcgg caccggcaaa cccatgaacg agcggaagca ctcgaggcaa
     1741 ccattgccgc gcggcatcac gaactccggc cagccctccg cgaggacctg gagccacgcg
     1801 tcctcgtggt tgccgcgcaa gcagacgacc ttggcagggg tggttctggt gagctctcgc
     1861 acccaggcga cgactcccgc actgtttgga ccccggtcga tgtagtcgcc gaggaagacc
     1921 acagtgtcgt cggcagcgag cgtcggcagg cgctcgaaga gctttgccaa gctcgcgaga
     1981 tccccgtgga tgtcacctat ggcaaaggtt cgacccggca ccgcatcatc ttagtgtagg
     2041 aaccgccaac gatcgcccgt tcgggtgtcg gttctggcga cgatcgacag ctcgcgtttt
     2101 cgctggcttg gagtggcacg aggcgtggac aataggagcg ccatgaagct tccgctcgtt
     2161 ctcgcctcgt tgttcgttgc ggccacggcg tatgcacagg ctcctggtga ctacgaggaa
     2221 ggcaccggcg cgccgggcat gagcgatccg gttgctccga cgccgccggc accgccgctg
     2281 cgcaaccagc gctggtcgat cggccttggc gttggctcga tgagcctcgc gccgcacaat
     2341 gcacccgatg
//
LOCUS       DASUFX010000393         6621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1313308, whole genome shotgun sequence.
ACCESSION   DASUFX010000393 DASUFX010000000
VERSION     DASUFX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313308"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFV99_15685"
     CDS             <1..563
                     /locus_tag="VFV99_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060810.1"
                     /translation="LLLVVGCAKKAAETGARNTEAVGGAPGAGAGSSAAVMATSPQDT
                     HQSEAAKAPPPPPAGGGPSQGAKGGEGGGSAIDQARGAGVLGPTDQQAFLVKGAVTIK
                     SASSKDLDAAVKEQLEALQACYDKSLEMNEKLAGDLTLAIKDAKASVSKTTVKDSDLE
                     QCIVHVLEKVKLPKGKSTLVLSFKRQ"
     gene            578..1207
                     /locus_tag="VFV99_15690"
     CDS             578..1207
                     /locus_tag="VFV99_15690"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEX5060811.1"
                     /translation="MVIRGSARVTCPACGAKHDGELVQSINTQTNPKDKERLLAGELN
                     VLACKCGKRTQLAANVLFHDPDAHYYCQVVPGGDQAMDQAAAAFAASGVSGTLRLVPS
                     LNALVEKVKLLDAGLVDWAIEMTKVLLLASIGELDRVLLFEAIDREAGVLRWVIFDEE
                     GRAPERVSSPLAAYEKLVTRTASKPADSELRIDRAWAVEAVKKMVAEAN"
     gene            1236..1451
                     /locus_tag="VFV99_15695"
     CDS             1236..1451
                     /locus_tag="VFV99_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060812.1"
                     /translation="MSKAKQPSPTGKRGSRDKHVPGDSSTERQDESTPEREESSLLDD
                     LFGESDGVVDEENELDTDKYARPVDEP"
     gene            1542..3686
                     /locus_tag="VFV99_15700"
     CDS             1542..3686
                     /locus_tag="VFV99_15700"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX5060813.1"
                     /translation="MRSYLFGALGLTAVASLLGPASSNAQAPTAIPAIATNPLAMTAN
                     KQCDAVRDLVIDTAVHQMIVGYGYGYGYNPYYGGYPGAIADEAMPTAQPSSKAAAAPV
                     AAPAPSAGRAMPVQQSAPTSGPSHYTTTNVHEKGVDEADLVKTDGKYVYTLRNNELIV
                     AKTWPVDKPDVVARLTFKTMVPTQLYLHGEQLVLQGYATQPRQNQYAYGSTRVIVVNA
                     HNRERPKLEKIFDVDGGTFSSRIVGDDMYLVQNGYLQMPPKLLESAQKVMAKIPRADQ
                     TSLRPWEIQSRLAATLRKAIYSNLTAQDVEAMLPAIYNGSQKTPLACDSLYMPPNNMQ
                     LGLTALARISLTSSKTDLVGAMVSGGTVYASTDSLYIASPFYQWNQQGYSNMQTQVHQ
                     FSLADKDGKPRYVASGAVDGNILNEFSMSEYNGDLRIATTDWQWNGQQGGNHLFVMRP
                     RGSKLEVIGAVRGLAKGERIYAGRMFGDRGYLVTFKQTDPLFTLDLSDPQHPRVAGEL
                     KINGFSNYIHPMGGNLLLTIGQDADENGRVKGMHLQMFDVNDATNPKRRFHETFALKN
                     GGNYSYSAAQNDHHAFTYDPFTGTLALPFYEYNSNTGTNYQGLIAYHVDAKRGFKSLG
                     RIDHGMLADKMIAHTCEQQKKLNNGNDVYYCNKANWKNMRSQYPIDRSIVVDKYLMTL
                     GTAGLMIHTIAGLDQVSMLSWSKIDDHAALAQ"
     gene            3758..4807
                     /locus_tag="VFV99_15705"
     CDS             3758..4807
                     /locus_tag="VFV99_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5060814.1"
                     /translation="MDPRFVALQKRAAECSDVIGLAGGLPADELLPRDELAQALADVT
                     ASRKDALQYGWPEGVEQLRTWIANRLAARGATVEPDRVIITAGAQQALSIAGAMFAGR
                     AIAVGAATYPAAIQAFLRAGARVVEHGGDARYVIVGVANPQGVALDESLGEIPLIVDE
                     AYAELRFDGELPPLLQAAAPDRVWHVGTISKTLAPGLRVGWLVPPAAEHEAALAIKHA
                     ADLQTASLSQAALARFLATFDYDHFAGRARAAYAERASALVTGMRRHAPMLEFTEPDG
                     GFSIWIETDVKGDEVALLKAALAEGVMVDPGSEFRALPDERIAFRVSYSHVGPEQLDE
                     GARRVARMFERFVRE"
     gene            complement(4797..5651)
                     /locus_tag="VFV99_15710"
     CDS             complement(4797..5651)
                     /locus_tag="VFV99_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060815.1"
                     /translation="MKGCALLALLVACGPGTRGGDGLGDDDQSMTDGSMNANECPMGA
                     ELVYVIDQFNYRISTFNPATKTFTDLGSLSCPAMGGAGPFSMSVDRQAIAWVLYDSGE
                     LFRVVLPSLACTKTTWNSGGELKVFGMGFSTDEPMGDKETLYVGGGANQMQTSYKLAK
                     FDTSSLTAQVIGTQTQLPEMTGNAKAELWGFMPDATNARVVQFDKTNGSALKTYMLPT
                     LAGTMTGYAFAHWGGDYWVFLIKNAEPSTTVYQVDGATGTIKSTTPTTGRTIVGAGVS
                     TCAPTVIL"
     gene            complement(5708..6253)
                     /locus_tag="VFV99_15715"
     CDS             complement(5708..6253)
                     /locus_tag="VFV99_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060816.1"
                     /translation="MTTLGDLFGAALVLPDAVDAPRAAAARARCDARGYTRYARYDRG
                     SYDVLENPDEPELVGLMVRAAERTTGRTLALAESRVLRLLPGDYVLAHHDRIHEGNPV
                     EVTLDLSPNPADADVHYRRRGQVFFRVPCIPGALAIVERGVTITRNHTYVSKLLRDAV
                     VVRLIVLLHDSPPAMHTTRSA"
     gene            complement(6250..>6621)
                     /locus_tag="VFV99_15720"
     CDS             complement(6250..>6621)
                     /locus_tag="VFV99_15720"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5060817.1"
                     /translation="EFAAVLAPPLSRITGKPVSRVDMRAYAYRPGHYLLPHSDHQDGL
                     TRALAYAYYVPSPEPPQGGELELFACRIERGEIVATDSARIIEPRGNRLVVFDVSDVS
                     LHQVREVLSGLRISLAGWFYP"
BASE COUNT         1261 a         2125 c         2169 g         1066 t
ORIGIN      
        1 ctctgttgct cgtcgtcggc tgtgcgaaga aagctgcgga gaccggtgcg cgtaataccg
       61 aagcggtcgg cggcgcgccg ggcgcaggcg ctggctcgtc tgctgcggtg atggccacta
      121 gtccgcaaga cacgcatcag agcgaagcag cgaaggcacc gcctcctccg ccggcgggag
      181 gcggcccatc gcagggagcg aagggtggcg agggaggagg ctctgcgatc gatcaggcgc
      241 gcggcgccgg cgtgctcggc ccgaccgatc agcaggcgtt cctggtcaaa ggtgcagtca
      301 cgatcaagag cgcgagctcg aaggatctcg atgccgcggt gaaggagcag ctcgaggcac
      361 tgcaagcctg ctacgacaaa tcactcgaga tgaacgagaa gctcgccggt gatctgacgc
      421 tcgcgatcaa ggacgcgaag gcgagcgtca gcaagacaac cgtcaaggac agcgacctcg
      481 agcagtgcat cgtgcacgtg ctggagaagg taaaactccc gaagggaaag tcgacgctgg
      541 tcctttcctt caagcgccag tgagctaatc cacccctatg gtcattcgcg gtagcgcgcg
      601 cgtcacctgt cccgcgtgcg gagcgaagca cgacggcgag ctcgtgcaga gcatcaacac
      661 gcagacgaat cccaaggaca aggaacgtct gctcgcgggt gagctcaacg tgctcgcctg
      721 caagtgtggc aagcgtacgc agctcgccgc gaacgtgctg ttccacgatc ccgatgcgca
      781 ctactactgc caagtcgtgc cgggcggcga tcaagcgatg gaccaagccg ccgcagcatt
      841 tgcagcgtcg ggtgtgagtg gcacgctgag gctcgtaccg tcactcaacg cactcgtcga
      901 gaaggtgaag ctgctcgacg ccgggctggt cgactgggcg atcgaaatga ccaaggtgct
      961 gctactcgca tcgatcggcg agctcgatcg cgtgctgttg ttcgaggcga tcgatcgtga
     1021 ggcaggagtg ctacggtggg tcatcttcga cgaggaggga cgtgcgccgg agcgcgtttc
     1081 cagcccgctg gccgcgtacg agaagctcgt gacacgcacc gcgagcaagc ccgccgactc
     1141 ggagttacgc atcgatagag cgtgggccgt cgaagcagtg aagaagatgg tcgcggaggc
     1201 gaactagggc atgcggcttg ctgaaacgag ccgccatgtc gaaagcgaag cagccgtccc
     1261 ctacgggcaa acgcgggagt cgcgacaaac atgtcccggg cgattcgagt accgagcgcc
     1321 aagacgagtc gaccccggag cgtgaagaat cgagcctgct cgacgatctg ttcggcgaga
     1381 gcgacggtgt cgtcgacgaa gagaacgagc tcgatacgga taagtacgcg cggccggtcg
     1441 acgaaccgtg aacgctcggt cacagcccac atcaccccac gggctgtttc cgacgagtta
     1501 aggcgctaga ccaaaccaac gtatgggccg tttgcatgcc catgcgtagc tatcttttcg
     1561 gcgccctcgg tttgaccgct gtcgcgtcgc tgctcggtcc cgcttcttcc aacgcccagg
     1621 ccccgacggc cattcccgca atcgcgacga acccgctcgc gatgacggcg aacaagcaat
     1681 gcgatgcggt gcgcgacctc gtgatcgaca ccgccgtcca ccagatgatc gtcggctacg
     1741 gctacggcta cgggtacaac ccgtactatg gcgggtatcc cggtgcgatc gccgatgagg
     1801 cgatgcccac ggcacagccg tcgagtaagg ctgcagcggc acccgtcgcg gctcctgcac
     1861 cgtccgccgg tcgcgcgatg cccgtgcagc agagcgcccc gacgtcgggt ccctcgcact
     1921 acacgacgac gaacgtgcat gagaagggcg tcgacgaggc agacctcgtc aagaccgacg
     1981 gcaagtatgt ctacacgctg cgcaacaacg agctgatcgt cgcgaagacg tggccggtcg
     2041 acaagccgga cgtcgtcgcg cgcctcacgt tcaagacgat ggtgccgacg cagctctacc
     2101 tgcacggcga gcagctcgtg ctgcagggct atgcgacgca gccgcgccag aaccagtacg
     2161 catacggttc gacgcgcgtg atcgtcgtca acgcgcacaa ccgcgagcgt ccgaagctcg
     2221 agaagatctt cgacgtcgac ggcggcacgt tcagctcgcg catcgtcggc gatgacatgt
     2281 acctcgtgca gaacggttat ctacagatgc cgccgaaact gctcgagagc gctcagaagg
     2341 tgatggcgaa gatcccgcgc gcggatcaga cctcgctgcg cccgtgggag atccagagcc
     2401 ggctcgccgc gacgctgcgt aaagcgatct acagcaacct gaccgcgcag gatgtcgaag
     2461 cgatgctgcc cgcgatttac aacggcagcc agaagacgcc gctcgcatgc gactcgctgt
     2521 acatgccgcc gaacaacatg cagctcggcc tcaccgcgct cgcgcgcatc tcgctgacgt
     2581 cgtcgaagac cgacctcgtc ggcgcgatgg tgtcgggcgg cacggtgtac gcatcgaccg
     2641 attcgctcta catcgcgtcg ccgttctacc agtggaacca gcagggctac tcgaacatgc
     2701 agacgcaggt ccaccagttc tcgctcgccg acaaggacgg gaagccgcgc tacgtcgcca
     2761 gcggcgcggt cgacggcaac atcctcaacg agttctcgat gagcgagtac aacggtgatc
     2821 tccgcatcgc gacgaccgat tggcagtgga atggtcagca gggcggcaat cacttgttcg
     2881 tgatgcgccc gcgcggcagc aagctcgaag tcatcggcgc ggtgcgtgga ctcgcgaagg
     2941 gcgagcgcat ctatgccggc cgcatgttcg gcgaccgcgg ctacctcgtc acgttcaagc
     3001 agacggaccc gctgttcacg ctcgacctca gcgatccgca gcacccgcgc gtcgccggcg
     3061 agctcaagat caacggcttc tcgaactaca tccacccgat gggcggcaac ctgctgctca
     3121 cgatcggtca ggatgccgac gagaacggcc gcgtgaaggg catgcacctg caaatgttcg
     3181 acgtcaacga cgcgacgaac ccgaagcgcc gcttccacga gacgttcgcg ctgaagaacg
     3241 gcggcaacta ctcgtactcg gcggcgcaga acgatcacca cgccttcacg tacgacccgt
     3301 tcaccggcac gctcgcgctg ccgttctacg agtacaacag caacaccggc acgaactacc
     3361 agggcctgat cgcctatcac gtcgacgcga agcgcggctt caagtcgctc ggccgcatcg
     3421 atcacggaat gctcgctgac aagatgatcg cgcatacctg cgagcagcag aagaagctca
     3481 acaacggcaa cgacgtctac tactgcaaca aggcgaactg gaagaacatg cgttcgcagt
     3541 atccgatcga tcgcagcatc gtcgtcgaca agtacctcat gacgctcggc acggccggcc
     3601 tcatgatcca cacgatcgcc ggtctcgatc aggtgtcgat gctgtcgtgg tcgaagatcg
     3661 atgaccacgc cgcactcgcg cagtaagcga gcgtaggcgc acgggcgagc gacatcgctc
     3721 gtaccggttg gcattcgtgt tgaaccaaac gcgggctgtg gacccgcgtt ttgtcgcgct
     3781 acaaaagcga gccgccgagt gcagcgatgt catcggcctt gcgggtggct tgccggccga
     3841 cgagctcctg ccgcgtgacg agcttgcgca ggcgctcgcg gacgtcactg catcgcgcaa
     3901 agacgcgctt cagtacggct ggccggaagg cgtcgagcag ctccgcacct ggatcgcgaa
     3961 tcgacttgcc gcacgaggcg ccacggtcga acccgaccgc gtcatcatca cggctggcgc
     4021 tcagcaggcg ttgtcgattg ccggcgcgat gttcgccggt cgcgcgatcg cagtcggcgc
     4081 ggcgacgtat ccagcggcga tccaggcgtt cctgcgcgcc ggcgctcgtg tcgtggagca
     4141 cggcggtgat gcgcgctacg taatagtggg cgttgccaac ccgcagggtg tagcgctcga
     4201 cgagagcctc ggcgagatac cgttgatcgt cgacgaggcg tacgccgagc tacgcttcga
     4261 tggcgagctg ccgccgctgt tgcaggccgc cgcacccgat cgcgtctggc acgtgggcac
     4321 gatcagcaag acactcgcgc cgggactgcg cgtcggctgg ctggtaccgc cggcggccga
     4381 gcacgaggcg gcgttagcaa tcaaacatgc cgccgatctg cagacagcga gcctctcgca
     4441 agcggcactc gcgcgattct tggctacgtt cgactacgac catttcgccg gcagagcgcg
     4501 cgcagcctat gcagagcgag caagcgcgct cgtcacgggc atgcgccgcc acgcaccgat
     4561 gctcgagttc accgaaccag acggtggctt ctcgatctgg atcgagaccg atgtgaaggg
     4621 cgacgaggtc gcgctactga aggcggcgct cgctgaaggc gtgatggtcg atccgggcag
     4681 cgagttccgc gcgctgccgg acgagcgcat cgcgtttcgc gtgagctact cgcacgtcgg
     4741 cccggagcag ctcgacgagg gcgcgcgcag agtcgcgcgg atgttcgagc gcttcgttag
     4801 agaatgacgg tcggcgcgca ggtcgacaca cccgcaccga cgatcgtgcg gccggtcgtc
     4861 ggcgtcgtgc tcttgatcgt gccggtcgca ccgtccactt gatacacggt ggtcgacggc
     4921 tcggcgttct tgatcaggaa cacccagtag tcgccgcccc agtgcgcgaa cgcgtagccc
     4981 gtcatcgtac cggcgagcgt cggcagcatg tacgtcttga gtgcagagcc gttggtcttg
     5041 tcgaactgca ccacgcgcgc gttcgtcgcg tcgggcatga agccccacag ctcggccttc
     5101 gcgttgccgg tcatctcggg cagctgggtc tgcgtgccga tcacctgcgc ggtgagcgag
     5161 ctcgtatcga acttggcgag cttgtagctc gtctgcatct ggttcgcgcc gccgccgacg
     5221 tacagcgtct ccttgtcgcc catcggctcg tccgtcgaga agcccatgcc gaacaccttg
     5281 agctcgccgc cgctgttcca cgttgtcttc gtgcacgcga ggcttggcag cacgacccgg
     5341 aacagctcgc cgctgtcgta gagcacccac gcgattgcct gtcgatcgac gctcatcgag
     5401 aatggacccg cgccacccat cgctgggcag ctgagcgatc cgagatcggt gaacgtcttg
     5461 gtcgccgggt tgaatgtcga gattcggtag ttgaactggt cgatcacgta gaccagctcg
     5521 gcgcccatcg gacactcgtt ggcgttcatc gagccgtcgg tcatcgactg atcgtcgtcg
     5581 cctaagccgt cgccgccgcg agtacccgga ccacaagcaa ccagcaacgc gagtagcgcg
     5641 catcccttca tcgcgagtca cgctagcgct ttcggatcgc cgccgaaact acaattcgtc
     5701 gacgatttca cgcgctgcgc gtggtgtgca tcgcgggtgg cgaatcgtgc agcaacacta
     5761 tgaggcgcac cacaactgcg tcgcgcagca gcttgctcac gtaggtgtgg ttgcgcgtga
     5821 tcgtcacgcc gcgttcgacg atcgcgagag cgccgggtat gcacggaacg cgaaagaaca
     5881 cctgaccgcg gcggcgatag tgcacgtccg cgtcggcggg attcggcgac agatcgagcg
     5941 tgacctcgac gggattgccc tcgtgtatgc gatcgtgatg cgcgagcacg tagtccccgg
     6001 gcaggaggcg cagcacgcgc gattcagcga gcgcgagcgt gcggcctgtc gtgcgttcag
     6061 cggcgcgcac catgagcccg acgagctccg gctcgtcggg attctcgagc acgtcgtagc
     6121 tgccgcgatc gtagcgggca tagcgcgtgt agccccgagc gtcgcagcgc gcgcgtgcgg
     6181 cggctgcgcg tggggcatcg accgcgtccg gcaggacgag ggcggcgccg aacagatcgc
     6241 cgagcgtcgt cacggataga accatccggc gagtgagatg cgcagtccag agagcacctc
     6301 gcgtacctgg tgaagcgaca cgtcgctgac gtcgaacacg acgaggcgat tgccgcgcgg
     6361 ttcgatgatg cgcgcgctgt cggtcgcgac gatctcgcca cgctcgattc gacacgcgaa
     6421 cagctcgagc tcgccgccct gtggtggctc gggagatggc acgtagtacg cgtacgcgag
     6481 cgcgcgcgtg agcccatctt ggtgatcgga gtgcggaagc aagtagtgcc ccgggcggta
     6541 ggcatacgcg cgcatgtcga cgcgcgagac gggcttgccg gtgatccgag acagtggcgg
     6601 ggcgagcacc gctgcgaatt c
//
LOCUS       DASUFX010000394         5763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_481448,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000394 DASUFX010000000
VERSION     DASUFX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481448"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFV99_15725"
     CDS             <1..370
                     /locus_tag="VFV99_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060818.1"
                     /translation="DDVDIACTIADGLELAGYQTYVAHDAFSALRLTEGFAPDVALLD
                     IGLPDIDGCELAARLRERHGKDLVMMCVTAYGDDAHRAKAAAAGVQAYFVKPVPLRTL
                     RAAIAKLLPDAAHDDAGLQM"
     gene            complement(428..1573)
                     /locus_tag="VFV99_15730"
     CDS             complement(428..1573)
                     /locus_tag="VFV99_15730"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5060819.1"
                     /translation="MSGDLKAAERIRGLVEADPIVLVEIAGAISSEGRMPPDAITDVA
                     RDHAVRLADADKQRLRDAMEQAILGRDVDLALEWMHQAGILRVLFPELEATVDLVQEA
                     GRQHKDVWAHTKQVVKQTVRRPLVRWAALLHDIGKVPTRTFTADGVHFHGHAEVGARM
                     FDRVYPRFAFARDERQTIRFLVKHHLRTNQYSEAWTDSAVRRFHREMGPHMIDLLDLS
                     RADITSKRPGRRKALLEQIAALADRVDRLVEEDAKQPPLPGGVGNAIMDAFELAPSRL
                     IGDLKRALETAIEAGTLEARREDAYYVAYIARQDLVPNVSPDKRDALIAAGGNIGAAA
                     EHDDPEGPHKGVDHDDPSPGVLACGHDPENDPCVHRDADPDDPDLHS"
     gene            1911..2720
                     /gene="murI"
                     /locus_tag="VFV99_15735"
     CDS             1911..2720
                     /gene="murI"
                     /locus_tag="VFV99_15735"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX5060820.1"
                     /translation="MTKRHPIGVFDSGVGGLTVVRALRRALPGEDIIYLGDTARVPYG
                     SKSPRTVEKYSLLCQRFLLDRSVKLVLIACNTASANALPALVEASPVPVIGAVEPGAA
                     SALAATKNGHIGVIGTLATVRSSAYAKAITARDPKAKLTALACPLFVPLAEEGWTDDE
                     VAVLIARRYLTQLFAQDPQIDTLVLGCTHYPLLRDVIANVAHELAGREIAVVDSASAM
                     AEAAKEALGSGPNKRTQAGRLDCFATDTSRLDELAPRFLGEALTGFELVDL"
     gene            complement(2763..3326)
                     /locus_tag="VFV99_15740"
     CDS             complement(2763..3326)
                     /locus_tag="VFV99_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060821.1"
                     /translation="MRSWLVSLPHDLKIAFDAMDDENLPRTVREIATGAAIYVVSPND
                     AVSDRNNNIVSYADDALLLRLALDKALGKGEDEQAFRDRFPELFDNLEDNLKLCKSVL
                     GDLVTWLESKVGGLKSIDYKGKKVPAYLDNDEAREALFDDGLVFRTDYPVDEKTIGDK
                     LKKAATVIETMKRRKADEARAKGLHAS"
     gene            3512..4921
                     /locus_tag="VFV99_15745"
     CDS             3512..4921
                     /locus_tag="VFV99_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060822.1"
                     /translation="MAKRSPILGYNHNVRYRGLVFHVQTEDSGLLNPHLFTHLFHEGV
                     IVSTRKLVYDAGSNEEAIKALMQAQHKAVMKDLRKGSFDDKIDQYLGGTPGLEPRASG
                     DAKPIPRGERAKTEADPAVAKAALDRTASPIEIINEDLSEPIELPVKARADSADRTIP
                     ITAIEPPPPGAFDGPTDRTGIEHPPSRTKTAQKVSKSTDIARAPTQHAPRDSAPVIEI
                     SVDDGGRARGPHDTEVSETQPRTTTPSRPPSHGAATLPAMKPPSRPIAPPVVTRPPTQ
                     DRKRPDSDVVEVYSPPPPSVEPPPGERAERPGQYSMSRKEGAAPIREKTGRINQINPA
                     NNQQGQRSDRGSGQIPKVEQRQTQARAAQPPPVPSQARGHTPAAGNKAVQQPQRVQQT
                     APIANRTPSPATPASGSGVVMTRPAVIVGAPPKPQGQPQRVRKAREDEGRGFGQGLIS
                     EKSLDEVILAYLSEDAEDK"
     gene            4983..5258
                     /locus_tag="VFV99_15750"
     CDS             4983..5258
                     /locus_tag="VFV99_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX5060823.1"
                     /translation="MAAHVKIFTTDWCGYCFAALRLLKQKGVAYEQLDSDDVRTRRWL
                     RDVTGQSTVPQIFIDGNSVGGYSDIRELDRRGVLDRLLAGSDDHPPH"
     gene            complement(5294..>5763)
                     /locus_tag="VFV99_15755"
     CDS             complement(5294..>5763)
                     /locus_tag="VFV99_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060824.1"
                     /translation="FSYYTEINLLTALGRVKEARNLLESRGPVPTGEVLKLSHWIAQM
                     HLWVTENAPQPVGDMPFKDPPKKKELEIDESELHDRMRKGLSMTAGADLLLLCAWCYA
                     YRGEHDDARFAYRQSKEREGSHRLDVAMPKLAAWIDEYRKEHPDLDHADADEP"
BASE COUNT         1016 a         1893 c         1861 g          993 t
ORIGIN      
        1 cgacgacgtg gacatcgctt gcacgattgc cgatggtctc gagctcgctg ggtaccagac
       61 gtacgtcgcg cacgatgcgt tcagtgcgct gcgcctcacg gagggcttcg cgcccgacgt
      121 cgccctgctc gacatcggac tccccgatat cgatggctgc gagctcgcgg cacggttgcg
      181 cgaacgccac ggcaaagatc tcgtcatgat gtgtgtcact gcctacggcg acgacgcgca
      241 tcgtgcgaaa gccgcggccg cgggcgtgca agcctacttc gtcaagccgg taccgctgcg
      301 tacgctgcgc gcggcgatcg cgaagctctt gcccgacgcg gcacacgacg acgccggact
      361 tcaaatgtaa aggctcaccc caggcgtcgc gaagatccgc ggcgaggagc cgtgcgcggt
      421 tcacgcgcta cgagtgcagg tctggatcgt cgggatccgc gtcgcggtgc acgcacggat
      481 cgttctcggg atcgtgaccg cacgcgagca cgcccggcga cggatcgtcg tgatcgacgc
      541 ccttgtgcgg accttcggga tcgtcgtgct cggcggctgc gccgatattg ccgcccgctg
      601 cgatcagcgc gtcgcgcttg tcgggcgaga cgttcggcac gagatcctgg cgcgcgatgt
      661 acgcgacgta gtacgcgtcc tcgcggcgcg cctcgagcgt gccggcttcg atggccgttt
      721 cgagcgcgcg cttgagatcg ccgatcagcc gcgacggtgc gagctcgaac gcatccatga
      781 tcgcgttgcc gacaccgcct ggtagcggcg gctgcttcgc gtcctcttcg acaaggcggt
      841 cgacgcgatc cgcgagcgca gcgatctgtt cgagcagcgc cttgcggcgg ccggggcgct
      901 tcgaggtgat gtcggcgcgc gacagatcga gaagatcgat catgtgcgga cccatctcgc
      961 gatggaaccg gcgcactgcg ctgtcggtcc acgcttcgct gtactggttc gtgcgcaggt
     1021 gatgcttgac gagaaagcgg atcgtttgcc gttcgtcgcg cgcgaacgcg aagcgcggat
     1081 acaccctgtc gaacatgcgc gcgccgactt ccgcgtgacc gtggaagtgc acgccgtccg
     1141 cagtgaacgt gcgcgtcgga actttgccga tgtcgtggag gagcgccgcc caccggacga
     1201 gcggacgccg gacggtctgc ttcacgactt gcttcgtgtg tgcccagacg tccttgtgct
     1261 ggcgacccgc ttcctgcacg aggtcgaccg tggcttcgag ctcggggaac agcacgcgca
     1321 ggatgccggc ctgatgcatc cactcgagcg cgaggtcgac gtcgcggccg agaatcgctt
     1381 gctccatcgc gtcgcgcagg cgctgcttgt cggcgtccgc gaggcggaca gcgtggtcgc
     1441 gagcgacatc ggtgatcgcg tccggaggca tgcgaccttc gctcgaaatc gcacctgcga
     1501 tctcgacgag cacgatcggg tcggcctcga cgaggccccg aatgcgctcg gccgccttca
     1561 gatcgccgct catataaggg tcggaacatc taccatgttt ggttggtacg ggccgtgctg
     1621 aatggcgcgg agccgtgcag ggtcgtatca ccgagagtca tctgccgcag gccgaggaat
     1681 cctcgcctgg tatcactcgt ttttacgagt ctctcgacga gaagcccgcg accttcctcg
     1741 atcttctctg ggcattcgag agtcgggtcc agcgttgtaa gaccgcacgg aagcgtcggc
     1801 gccgttagcg cgttttccta gcgacacgca tagccagctg gcaggttcag tggccagatg
     1861 gtgacagcgt ggcgcactcc ggagacgcaa tgaggcaaga ttccgcgacg atgacgaagc
     1921 gtcacccgat cggtgtattc gattcgggcg ttggtgggct cacggtcgta cgagcgctga
     1981 gacgagcgct tcccggcgaa gacatcattt atctcggcga cacggcacgc gttccttatg
     2041 gaagtaagag tccgcgcacg gtcgagaagt attcgctcct ctgtcagcgc tttctgctcg
     2101 atcgcagcgt caagctcgtc ttgatcgcgt gcaacacggc gagtgcgaac gcgctgcccg
     2161 cactcgtcga agcgagcccg gtgcccgtga tcggtgccgt cgagcccggc gctgcgagcg
     2221 cgctcgccgc gacgaagaac ggacacatcg gcgtgatcgg tacgctcgcc accgtacgtt
     2281 cgagtgcgta cgcaaaagcg atcaccgcgc gcgatccgaa ggcgaagctg accgcgctgg
     2341 cgtgtccgct gttcgtgccg ctcgccgaag aaggctggac cgacgacgaa gtcgccgtgc
     2401 tcatcgcgcg ccgctatctc acgcagctgt tcgcacagga tccgcagatc gacaccctcg
     2461 tgctcggctg cacgcactat ccgctgctgc gtgatgtgat cgcgaacgtc gcgcacgagc
     2521 ttgcaggtcg cgagatcgca gtcgtcgatt cggcgagcgc gatggcggag gctgcgaagg
     2581 aagcgcttgg cagcgggccg aacaagcgca cgcaagcggg tcgcctcgat tgcttcgcga
     2641 ccgacacctc gcgactcgac gagctcgcgc cacgctttct cggtgaagcg ctcaccggct
     2701 tcgagctcgt cgatctctga tcgaacgatt ctgaccgcgc gactgatctc tgatcgaggt
     2761 cgttacgaag cgtgcaggcc cttggcccgc gcttcgtccg ccttgcgacg cttcatcgtt
     2821 tcgatgaccg tcgctgcctt cttgagcttg tcgccgatcg tcttctcgtc gacgggatag
     2881 tcggtgcgga acacgaggcc gtcgtcgaac agcgcctcgc gcgcttcgtc gttgtcgagg
     2941 tacgcgggca ccttcttgcc cttgtagtcg atcgacttca ggccgccgac cttcgactcg
     3001 agccacgtca cgagatcacc aagcactgac ttgcagagct tgaggttgtc ctcgaggttg
     3061 tcgaacagct ccgggaagcg atcgcggaac gcctgctcgt cttcgccctt accgagcgcc
     3121 ttgtcgagcg cgaggcgcag aagtagcgcg tcgtccgcgt agctcacgat gttgttgttg
     3181 cgatcggaga ccgcatcatt cggagacacg acgtagatgg cggcgccggt cgcaatttcc
     3241 ctgaccgtcc gcggtaggtt ttcgtcgtcc atcgcatcaa acgcgatctt gaggtcgtga
     3301 gggagagaaa caagccaaga cctcatcaac tcgacgaact tcgactccgc tgtgatcgtc
     3361 acgggatatg cctaccccgc agttgcgggg cctccaaatc agcttcgaaa ttgaagcccg
     3421 aagagggtga gtaccaaccc cgaaggggaa ttacaaggcg gacttgggcg accgagcgcc
     3481 gttttgcggg ccgtggtacc ctcgaaaccc ggtggccaag cgttcaccca tcctcggtta
     3541 caaccacaac gtcaggtacc gaggcctggt tttccacgtc caaaccgagg actcggggtt
     3601 actcaacccc cacctgttca cgcacttgtt ccacgagggc gtgatcgtct cgacccgcaa
     3661 gctcgtctac gacgcgggct ccaacgagga ggcgatcaag gccctgatgc aggctcagca
     3721 caaggccgtg atgaaggatc tcaggaaggg gtccttcgac gacaagatcg accagtacct
     3781 tggcggtacc cccggtctcg agccgcgcgc cagtggtgac gcgaagccga tcccgcgcgg
     3841 cgaacgcgcg aagaccgagg ccgacccggc ggtcgccaag gcggcgctcg atcgcacagc
     3901 gtcgcccatc gagatcatca acgaagacct gtccgagccg atcgagcttc cggtcaaggc
     3961 gcgcgccgac tccgctgacc gcacgattcc gatcaccgcg atcgagccac cgccaccggg
     4021 cgcgttcgat ggcccgaccg atcgcacggg catcgagcat ccgccgtcgc gcacgaagac
     4081 cgcgcagaag gtttcgaagt cgaccgacat cgcccgcgct ccgacgcagc acgcgcctcg
     4141 cgattcggcg ccggtgatcg agatcagcgt cgacgatggt ggtcgcgcgc gtggtccgca
     4201 cgacaccgaa gtttcggaga cgcaaccgcg cacgacgacg ccgagccgcc cgccgagtca
     4261 cggcgctgcg acgctgccgg caatgaagcc gccgtcgcgt ccaatcgcgc cgccggtcgt
     4321 gacgcggccg ccgacgcaag atcgcaagcg ccccgacagc gatgtcgtcg aggtctactc
     4381 gccgccgccg ccgtccgtcg agccaccgcc gggagaacgc gccgagcgcc cgggtcagta
     4441 ctcgatgagt cgcaaagaag gtgcggcgcc gattcgcgag aagaccggcc gcatcaatca
     4501 gatcaatccg gcgaacaatc agcaggggca gcgatcggat cgcggctccg gtcagatccc
     4561 gaaggtcgag cagcgccaga cccaagcacg cgcagcacaa ccgccgccgg ttccatcaca
     4621 agcgcgcggc cacacgccgg cagccggaaa caaggcagtg cagcaaccac agcgcgtcca
     4681 acagacggcg ccgatcgcga atcgcacgcc atcgccggcg acgccagcgt cgggcagcgg
     4741 cgtcgtgatg acgcgcccag cggtgatcgt cggcgcgcct ccgaagccgc aaggccagcc
     4801 gcagcgcgtg cgaaaagctc gcgaggatga agggcgtggc ttcgggcaag gcctgatctc
     4861 ggagaagagc ctcgacgaag tgatcctcgc gtatttgtcc gaagacgccg aggacaagta
     4921 gcgcgagctc ctcgtacgtg aggtgcgctg gcgctctacg cattcgccag tacggttcga
     4981 acatggccgc gcacgtgaag atctttacga ccgattggtg tggttactgc ttcgccgcgc
     5041 tgcgcctgtt gaagcagaag ggtgtcgcgt acgagcagct cgattccgac gatgtccgga
     5101 cgcgacgctg gctacgcgat gtcaccggcc agtcgacggt gccgcagatc ttcatcgacg
     5161 gaaactcggt cggcgggtac agcgacattc gcgagctcga ccggcgcggc gtgctcgatc
     5221 gcttgctcgc tggcagcgac gatcacccgc cgcactgatc cgagcttgcc cgaagcccga
     5281 agcccgaagc cggctacggc tcgtcagcgt cggcgtgatc gagatcgggg tgctctttgc
     5341 ggtactcgtc gatccacgcc gcgagcttgg gcatcgcgac atccagccga tgcgagccct
     5401 cgcgctcctt tgactggcga tacgcgaacc gcgcgtcgtc gtgctcgccg cgatacgcgt
     5461 aacaccacgc gcacagcagc agcagatcgg cgcctgcggt catcgacaga cccttgcgca
     5521 tccgatcgtg gagctcgctc tcgtcgatct cgagctcctt cttcttcggc ggatctttga
     5581 agggcatgtc gccaaccggc tgcggcgcat tctcggtgac ccacagatgc atctgtgcga
     5641 tccagtgcga tagcttgagc acttcgcccg tcggcaccgg gccgcgcgac tcgagcaggt
     5701 tgcgcgcttc cttcacgcgc ccgagcgcgg tgagcaagtt gatctcggtg tagtacgaga
     5761 att
//
LOCUS       DASUFX010000395         6091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_63248,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000395 DASUFX010000000
VERSION     DASUFX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63248"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFV99_15760"
     CDS             <1..781
                     /locus_tag="VFV99_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060825.1"
                     /translation="SMADPRLPTVSTRLPAAFPPRWGFFKGLLTGAVIEVPALAIGVW
                     LLARLGIGDRTAPFMKILRLGAVFAGVAALFTAAGVGRLAAYASVDKIGGRRHAMRVA
                     AQAHAAAGAGLLLIATIPNGHLPEHVIGWFAILAMGGLVGAGCGAAIGAVCGGAAPVS
                     IGDVMAVAIKRPSEALRQLLDPEDLVKLGAAVRKRTTTLLGGMFEPAERPPGEEPLQP
                     FPEGKVLASERADRKPTDKPKDKSTTASADKPAEPPAAPRE"
     gene            783..1469
                     /locus_tag="VFV99_15765"
                     /pseudo
     CDS             783..1469
                     /locus_tag="VFV99_15765"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
     gene            complement(1492..2109)
                     /locus_tag="VFV99_15770"
     CDS             complement(1492..2109)
                     /locus_tag="VFV99_15770"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5060826.1"
                     /translation="MRLAIAIVLLAAQTAHADKRESCYDELDARHISFTKMKKPGIAN
                     AIEVTGMLGGIEVTGEGALVIDCSLAVSLDEAGRYMHALGIVKATFASAYSRRNVRGT
                     NHPSKHSYGLAIDVSGFSGPDLGSMRVARDYEQGLGDAVDCVGQPMTQAGAVLKVLQC
                     QLVRSGLFHLVLSPDYDDAHHDHFHLEALPWRERSSLRASTPAIH"
     gene            complement(2148..4310)
                     /locus_tag="VFV99_15775"
     CDS             complement(2148..4310)
                     /locus_tag="VFV99_15775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5060827.1"
                     /translation="MYYELALISVLIAGGYWGWYFLRHEQTRLYGFLQLAAAGLCTLG
                     LIGNRFDKPGLGIPGAIGVGAGVCFLILGPLARGMARRFSAAERFGAAEKMLDVADIL
                     APGSGVGDEKALLYAMREIRDGNIEQTVDALTAAKDRAPADARLAIDERIAMLYLASY
                     RWEEAIAHAEEHLFGALPPVSETPGSHVALRRVLGIAPPVWVELLGAYGYKGDLDQAA
                     LMLARLEEVCAGRPDAGIWLHRGRLIFLALAGRVGAVQTLVEPKRSRHMKAAARQYWI
                     AVAHERMGEVATAEAAYERARMRTRGRPRVLIDQALERLKTAKRTELGPTATEVVARV
                     EAEPPPVVTERVRPRGPLATRTLVAVCLLAATVIAFVLDGSTDVGTLMRAGAMVRSLV
                     ADGEWWRLVSCIFIHVGGIHLIVNMIGLWFLGRLVEDLFGPSQMIAIFAVSGIAGAAA
                     SYLASPVGASAGASGAIFGLLGAVFIELSLHRKQHRVAWSRGVWGSLAVVTVAQIAIG
                     FFYPVIDQWAHGAGLAAGAIAGILLSPNVRWPKLGLYAARAIAIVFGLLVVTSAVFVV
                     RTSIADSLANEPLERRQSINVSVVVPVSWQRSTDGELFDPDLYIPLLAKRVPAIGTLD
                     AQVEAYRKDEEKHATDTKGFEKVTAATDQLIPLPAEWRGSEMLASMPDPLGDHQQFRV
                     VVAGRPHPQGVILVSLYIPETVARAAPAWFTQLLASVQ"
     gene            complement(4315..5304)
                     /locus_tag="VFV99_15780"
     CDS             complement(4315..5304)
                     /locus_tag="VFV99_15780"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5060828.1"
                     /translation="MANIEPPQLTDRMILEFERPIIDLEKKISELRGLSTETVDFSAE
                     IRKLEQKARKLQKEVFAELSAQQKVQLARHPARPYMMDYVGLLMDDFVELHGDRSFRD
                     DPAIVGGLAQFDEWEVLVLGHQKGRNTKDNMHRNFGMARPEGYRKATRLMRMASRFKR
                     PIICFIDTPGAYPGLGAEERGQAEAIAKALQVMASLECPMISVVIGEGGSGGALALGV
                     TDRILMFEYSIYSVISPEGCASILWRDPAKISEAATQLKLTAPDLVQLGICDEIIPEA
                     AGGAHRNGAQTAAKLRSALKKHLRELVELSPNELVERRYQKFRKMGAFVEAPS"
     gene            complement(5313..>6091)
                     /gene="miaA"
                     /locus_tag="VFV99_15785"
     CDS             complement(5313..>6091)
                     /gene="miaA"
                     /locus_tag="VFV99_15785"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5060829.1"
                     /translation="GTGKVSVDERARVPHHLLDVVRPDEEMTAARFIELADKAIADVA
                     ARGRVPIICGGTGLYVRALLLGLFEGPPASADVRRELIALGIEALYAELSRVDPESAK
                     RIDRNDEKRIVRALEVYRLTGDTMSAHQARHDHKTMPRRYPARLVGLSPEREKLYAAI
                     DARVDEMIALGLEREVEALRTAGYAPPLRSQQAIGYAELHDVAKGTLERGRAIELIKR
                     NSRHYARRQISWYRPDQTITWYASPADVDLAELERYLAGL"
BASE COUNT          994 a         2058 c         2033 g         1006 t
ORIGIN      
        1 gtctatggct gatcccaggc tccccactgt aagtacgcgg cttcctgcgg cttttccgcc
       61 gcgttggggg ttcttcaagg gccttctgac cggcgcggtc atcgaggtgc ccgcattggc
      121 gatcggcgtg tggctgctcg cgcgtctcgg catcggcgat cgcaccgctc cgttcatgaa
      181 gatcctgcgg ctgggtgcgg tgttcgcggg ggtcgctgcg ttgttcaccg cagcgggagt
      241 tggtcggctc gcggcctacg catcggtcga caagatcggc gggcggcgcc acgcgatgcg
      301 cgtcgcggcg caggcacatg cagcggccgg cgccggtctc ttgctcatcg ccacgatccc
      361 gaatggccac ctgcccgagc acgtgatcgg atggttcgcg atcctcgcga tgggcggctt
      421 ggtcggcgcg ggctgtggcg cggcgatcgg cgcggtgtgt ggcggcgcgg caccggtgag
      481 catcggtgac gtgatggccg tcgcgatcaa gcggccgagc gaggcactgc gtcagctgct
      541 cgatccggaa gatctggtca agctcggcgc cgcggtgcgc aagcgaacga cgacgctgct
      601 cggcggcatg ttcgagcccg cggaacgtcc gcccggcgag gaaccgttgc aaccgttccc
      661 ggaaggcaaa gtgctcgcga gcgagcgcgc cgaccgcaag ccgaccgaca agccgaagga
      721 caagtcgacg acagcaagcg cggacaaacc ggccgagccg cccgccgcgc cgcgagagtg
      781 acgtgcgcga cgaacgccac gagctccgca cgctagcgct cgcgctcctc gcgtcgctct
      841 tgctgtggaa cctgccgttc ggcggcgtgc tgctctatcc gttcaagctg ttcgcgacct
      901 ggctacacga gctctcgcac ggcctcgcga tgatcatgac cggcgccggc ttcgatcaca
      961 tcgtcgtcta tcgcgacacg agcggcctcg cgtacgcgta cacctcggtc ggatcgtttg
     1021 gcaccgcgat catcgcagca gccggttaca tgggcacgcc gctgtggggc gcggtgatgc
     1081 tcgtcgtcac accgacgccg aaggccgcgc ggcgcgcatt gctcgtgctc gccgggctgc
     1141 tcgtcggtac gacgatcctc gtgatcgcgc cgagcccggc cggtgacacg ttcgggcagt
     1201 gggcgattcc cgcgatcggc gccggcgtcg cgctgtgcgg cttgatcgtg cccgatcgct
     1261 ggcgcgtgct cgtcgcgcac ttcatcgcgg tgcaggcgtg cgtcaatgcg ctgctcgata
     1321 ttcgcgtgct gctgcggccg tcgcaggtcg tcgacggtaa atcggccggc aactccgacg
     1381 ctcacaacat ggcgctcaac acgttcggca cgaacgcgac gtgggcggtg tggacgtggg
     1441 ccatcatctg gctcgtctgg tcgctcgcgg tgctctacat cgcgctgcga atcagtggat
     1501 cgcgggcgtc gaagcgcgca gcgaggaccg ctcgcgccac ggcagcgctt cgagatgaaa
     1561 gtgatcgtga tgagcgtcgt cgtagtccgg tgagagcacc aggtggaaca agcccgagcg
     1621 caccagctga cactgcagaa ccttgaggac cgcgccggct tgcgtcatgg gctggccgac
     1681 gcagtcgacc gcatcgccga gaccttgctc gtaatcgcga gcgactcgca tcgagccgag
     1741 gtcggggccg gagaaaccac tgacgtcgat cgcgagcccg tagctgtgct ttgacggatg
     1801 attcgtgccg cgcacgttgc ggcgtgaata cgcggacgcg aacgtcgcct tcacgatgcc
     1861 gagcgcgtgc atgtagcggc cggcctcgtc gagcgacacc gcgagcgagc aatcgatcac
     1921 gagcgcgccc tcgcctgtca cctcgatgcc gccaagcatg ccggtcactt cgatcgcgtt
     1981 cgcgatgccg ggcttcttca tcttcgtgaa gctgatgtga cgcgcatcga gctcgtcgta
     2041 acagctctcg cgcttgtcgg catgcgccgt ctgggcggcg agcagcacga tggcaatagc
     2101 gaggcgcacg acgcgagcgt actcctcgcc gccggggtgc tccaacttta ctgtaccgac
     2161 gcgagcagct gcgtgaacca cgccggcgcc gcgcgggcaa cggtctcggg gatgtagagg
     2221 ctgacgagga tgacgccttg tggatgcggc ctgcctgcga cgacgacgcg gaactgctgg
     2281 tgatcgccga gcggatctgg catcgacgcg agcatctcgc taccgcgcca ctcggccggc
     2341 aacggaatca actggtcggt cgcagccgtg accttctcga accccttggt gtctgtcgca
     2401 tgcttctctt cgtctttgcg ataggcctcg acctgcgcgt cgagcgtacc gatcgctggg
     2461 acccgctttg cgagcagcgg gatgtagaga tctggatcga acagctcgcc gtccgtcgag
     2521 cgctgccacg acaccggcac gaccaccgac acattgatgc tctgccggcg ttcgagcggt
     2581 tcgttcgcga ggctgtcggc gattgacgtg cggacgacga acaccgcaga cgtgacgacg
     2641 agcaggccaa acacgatcgc gatcgcacgc gcggcgtaca ggccgagctt cggccaccgc
     2701 acgttcggcg agagcaggat cccggcgatg gcgcccgcgg caagaccggc gccgtgcgcc
     2761 cactgatcga tcaccggata gaagaagccg atcgcgatct gcgcgacggt gacgacggcg
     2821 aggctgcccc agacgccgcg cgaccacgcg acgcgatgct gcttgcgatg cagcgacagc
     2881 tcgatgaaca ccgcgccaag caggccgaag attgcacccg aggcaccggc cgaagcgccc
     2941 acaggtgatg ccaggtagct cgctgctgcg cccgcaatgc ccgagaccgc aaagatcgcg
     3001 atcatctgcg acggcccgaa cagatcctcg acgagccggc cgaggaacca caggccgatc
     3061 atgttcacga tcaggtgaat gccgccgacg tgaatgaaaa tgcagctgac gagtcgccac
     3121 cactcgccgt cggcgacgag gctgcgcacc attgcaccag cgcgcatcag cgtgccgacg
     3181 tcggtcgagc cgtcgagcac gaacgcgatc acggttgccg cgagcaggca cacggcgacg
     3241 agcgtgcgcg ttgccagcgg gccgcgcgga cgcacccgct cggtgacaac cggcggcggc
     3301 tccgcttcaa cgcgcgcgac cacctcggtc gccgtcgggc cgagctcggt gcgcttcgcg
     3361 gtcttcaatc gctcgagcgc ttgatcgatc agcacgcgcg ggcgaccacg cgtgcgcatt
     3421 cgcgccctct cgtatgccgc ttcggcagtt gcgacctcgc ccatgcgttc atgcgcgacg
     3481 gcgatccagt actggcgcgc ggcggccttc atgtggcgcg agcgcttcgg ctcgacgagc
     3541 gtctgcaccg cgccgacgcg accggcgagc gcgaggaaga tcagcctacc gcgatgcagc
     3601 cagatgcctg cgtcgggacg acccgcgcac acctcttcga ggcgcgcgag catgagcgcg
     3661 gcctgatcga gatcaccttt gtagccgtac gcgccgagca gctcgaccca caccggcggc
     3721 gcgatgccga gcacgcggcg cagcgcgacg tgcgaacccg gcgtttcgct gaccggtggc
     3781 agcgcaccga acagatgttc ctcggcatgc gcgatcgctt cttcccagcg atacgacgcg
     3841 aggtacagca tcgcgatgcg ctcgtcgatc gcgagccgcg cgtcggcggg tgcacggtcc
     3901 tttgcagcgg tcagcgcgtc gacggtctgc tcgatgttgc cgtcgcggat ctcgcgcatc
     3961 gcgtagagca gcgccttctc gtcgccgaca cccgaacccg gcgcgaggat gtcagcgacg
     4021 tcgagcatct tctcggccgc gccaaagcgc tcggccgcgg agaacctgcg ggccatgcca
     4081 cgcgccagcg ggccgaggat caggaagcac acgccagcgc cgacgccgat cgcgcccggg
     4141 atgccgaggc cgggcttatc gaaccgatta ccgatgaggc cgagcgtgca caggccggcg
     4201 gcggcgagct gcaagaaccc gtagagccgg gtctgctcgt gacgaaggaa ataccaaccc
     4261 cagtacccgc ccgcgatcag taccgagatc agcgcgagct cgtaatacat ggcgtcagga
     4321 cggcgcctcg acgaacgcgc ccatcttgcg gaacttctgg tagcgccgct cgacgagctc
     4381 gttcggggac agctcgacga gctcgcgcaa gtgcttcttg agcgcgctgc gcagcttcgc
     4441 cgcggtctgc gcgccgttgc gatgcgcgcc accggcagcc tcgggaatga tctcgtcgca
     4501 gatgccgagc tgcaccaggt cgggcgccgt cagcttcagc tgcgttgccg cttcgctgat
     4561 cttcgccggg tcgcgccaca ggatcgacgc gcagccctcg ggcgagatca ccgagtagat
     4621 cgagtactcg aacatcaaga tgcgatcggt gacgccgagt gcgagcgcgc cacccgagcc
     4681 accctcgccg atcacgacgg agatcatcgg gcactcgagg ctcgccatca cttgcagcgc
     4741 cttcgcgatc gcttcggcct gaccgcgctc ctcggcgccg aggccgggat acgcgcccgg
     4801 cgtatcgatg aagcagatga tcgggcgctt gaaccgcgac gccatgcgca tgaggcgcgt
     4861 tgccttgcga tagccctcgg gacgcgccat gccgaagtta cggtgcatgt tgtccttcgt
     4921 gttgcggccc ttctggtggc cgagcacgag gacttcccac tcgtcgaatt gcgcgaggcc
     4981 gccgacgatc gccggatcgt cgcgaaagct tcgatcgccg tgtagctcga cgaagtcgtc
     5041 catcagcaag ccgacgtaat ccatcatgta cggccgtgct ggatgacgcg cgagctgcac
     5101 cttctgttgc gcgctcagct ccgcgaacac ttccttctgg agcttgcggg ccttctgctc
     5161 gagcttgcga atctcggccg agaagtcgac cgtctcggtc gacagaccgc gcagctcgct
     5221 gatcttcttt tcgagatcga tgatcggccg ctcgaactcg aggatcatgc ggtcggtcag
     5281 ctggggcggt tcaatgttgg ccatcgtgtc gcctagagcc ctgctaggta ccgctccaat
     5341 tccgcgaggt caacgtcggc cggcgaggcg taccaggtga tcgtctgatc aggccggtac
     5401 cacgagattt gacgccgcgc gtaatgacgt gaattccgct tgatcaactc aatggcccga
     5461 ccacgttcta gcgttccttt tgccacgtcg tggagctcgg cgtagccgat cgcctgctgc
     5521 gaacgaagcg gaggcgcgta gccggcggtg cggagcgctt cgacctcgcg ctcgagcccc
     5581 agcgcgatca tctcgtccac acgcgcgtcg atcgcggcgt agagcttctc gcgctccggc
     5641 gacagcccga cgagccgcgc cggatagcgc ctcggcatcg tcttgtgatc gtgccgcgcc
     5701 tggtgcgcgc tcatcgtgtc gccagtcagt cgatacacct cgagcgcacg gacgattcgc
     5761 ttctcgtcgt tgcgatcgat gcgcttcgcg ctctccggat cgacgcgcga tagctcggcg
     5821 tacagcgcct cgatgccgag cgcgatcagc tcgcgtcgca cgtcggccga ggcgggcggg
     5881 ccttcgaaca gcccgagcag cagcgcacgc acgtagaggc cggtaccgcc gcagatgatc
     5941 ggtacgcgtc cgcgtgcagc gacgtcggcg atcgctttgt cggcgagctc gataaaccga
     6001 gccgcggtca tctcctcgtc tgggcgcacg acatcgagca ggtgatgcgg cacgcgcgcg
     6061 cgctcatcga cactgacctt gccggtgccg a
//
LOCUS       DASUFX010000396         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1452191, whole genome shotgun sequence.
ACCESSION   DASUFX010000396 DASUFX010000000
VERSION     DASUFX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452191"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFV99_15790"
     CDS             <1..319
                     /locus_tag="VFV99_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060830.1"
                     /translation="MGVLFDNKDGSSKDSAEAAGGVIGALLFAGLPAIWAFRAARNAS
                     RATRAANVAKSDPSYTWRLSGKFVIATDGPGVPHPELSFKVNGKIRTMLLALPQAEIV
                     NRS"
     gene            complement(316..999)
                     /locus_tag="VFV99_15795"
     CDS             complement(316..999)
                     /locus_tag="VFV99_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060831.1"
                     /translation="MSSGKLVGVVVAHLVAIAGCYQPELRDCTVHCSAPTDCTGGQVC
                     RNDGWCAMPDVKKCGERTSDQDASMEGSSTSDASQSSPDASLSCQQACTNGTCQNGVC
                     VIDCSIALSCTMTDIKCPPNVPCRVECGYHACSRKVLCGMSTSCEVDCIGEQSCGDDI
                     ECNTNRCEIYCIGTSSCAHRVRCSNACACDVTCSGAGSCAQPSECPATTCRIGNGCSS
                     TLAAGCDGC"
     gene            complement(1093..2163)
                     /gene="mnmA"
                     /locus_tag="VFV99_15800"
     CDS             complement(1093..2163)
                     /gene="mnmA"
                     /locus_tag="VFV99_15800"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5060832.1"
                     /translation="MSSQSPLVVAALSGGVDSAAAAALLVEQGHRVVGMTMRLYDARG
                     TAASSGGRCCGPRDIEDARAVCAHLGIPHYVIDLAAEFGDAVVEDFVEAYLAGETPNP
                     CVKCNQHIKFTPLLQRARAIGADVLVTGHYAQIIDGQLYRGLDAGKDQSYFLFSMPGH
                     ELTSVRFPLGGMTKDEVREHALRLGLPNAVKPESQEICFVPDGDYAGFVAATALRRGR
                     TLPTTGTIVHEDGRVVGQHEGVHKFTVGQHRGLGNLTTKDKLFVTAIDPARAEVRVGP
                     REAAEKHELAVRDLRWLSAPQTALTAQVQVRHRGTPISADVTVEGDHATVRLSAPTVA
                     APGQAAVFYAGDRVLAGGWIAR"
     gene            2202..3527
                     /gene="rlmD"
                     /locus_tag="VFV99_15805"
     CDS             2202..3527
                     /gene="rlmD"
                     /locus_tag="VFV99_15805"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5060833.1"
                     /translation="MNKCQVTAASLDETGHGVGLAEGIRVHVADLLPGERAEVAIDHA
                     SPHKPEAWGRIVRRLGPTSDQRIPPACPGFGRCGGCLWQHLSYPSQLEAKRARVVAAL
                     GPDAKVGDVRPSPQLFGYRNKGKYVVGKSGDHVLLGAYAPRSHHVIDTLGCRVVAPII
                     DEVATWVRGAAERAQLVPYDEATRVGELRYVVIREAAGDVMIALIVAPNTPRAKLEHV
                     ANALAKHPAVRSLVAITNDRRDGAIVPSGSSAQVLFGHGHLVEELAGVKLEVGASEFL
                     QVNRAQAQAMYTRVAELAGDVRGKRAIDLFAGLGGFGLHLARAGAEVIAVEIDRDAIS
                     QLRRAAERAGLPLTTIAGDASADLAPQLGRPDVVVVNPPRKGLSESTRELLATIAPAT
                     IIYISCGPESLGRDLDALRVQGYAPDAIEPFDLMPGTAQVETIVRLRRS"
     gene            3567..3773
                     /locus_tag="VFV99_15810"
     CDS             3567..3773
                     /locus_tag="VFV99_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060834.1"
                     /translation="MTSRDSDAWMNGPDPHARPLLTDLERYTGEHYRPQMAEPRHAEV
                     SLAKVAVPAAIGILIVLALLLVNR"
     gene            complement(3777..>4562)
                     /locus_tag="VFV99_15815"
     CDS             complement(3777..>4562)
                     /locus_tag="VFV99_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060835.1"
                     /translation="GCVPLDEHLLFSTGWEWGYWLNDVVSLRASYEQGSSAEDLLTDA
                     YAPDLGVAAGHFVGRLMAAQKKALIDQRLIGYLVGRDIAIDAGDRLDPPIISQPDRIT
                     FEDLVANRQLEAALIIVLADLDAYATTLDGLESELDALDLPDSRWSSELRDGVAIDRL
                     RVRFVNATYNAVLAFVRNQNFAPDYEKAKELLARARKLVASRHADLHDNHGRRLVDKT
                     ANHTFYQFGYLYMADTLCYWQRELDQVGQILGTSTAVPNTCLF"
BASE COUNT          748 a         1486 c         1572 g          756 t
ORIGIN      
        1 gatgggtgtc ctgttcgaca acaaagacgg tagctcgaaa gactctgcgg aagccgccgg
       61 cggagtgatt ggcgccctgc tgttcgcagg cctgcccgcc atctgggcgt ttcgtgcagc
      121 gcgcaacgcg tctcgcgcga cccgggccgc gaatgttgcg aagagcgatc cgagctacac
      181 gtggcgtctg tcgggcaagt tcgtcatcgc gaccgacggc cccggcgtgc cgcatcccga
      241 gctgtcgttc aaggtgaacg gcaagatccg cacgatgctg ctcgcgctgc cgcaggcaga
      301 aatcgtcaat cgcagctagc agccgtcgca accggctgcg agcgtgctcg aacagccgtt
      361 cccgatccgg catgtggtgg ccgggcactc ggacggttgg gcgcacgatc cggcgccact
      421 gcacgtgacg tcacaggcgc atgcgttcga gcaccgcacg cggtgcgcac acgagctcgt
      481 tccgatgcag tagatctcgc agcgattcgt gttgcattcg atgtcgtctc cacacgactg
      541 ctcgccgatg cagtcgacct cgcacgacgt cgacatgccg cacagaacct tgcggctgca
      601 cgcgtggtag ccgcactcga cgcggcacgg cacgttcggc gggcatttga tgtccgtcat
      661 cgtgcacgac aacgcgatgc tgcaatcgat cacgcaaacg ccgttctggc acgtgccgtt
      721 cgtgcacgcc tgctggcagc taagcgatgc gtcggggctc gactgcgacg catcggacgt
      781 actgctacct tccatgctcg cgtcttggtc gctggttcgc tcgccgcact tcttgacgtc
      841 gggcatcgcg caccagccgt cgttgcgaca cacctgaccg ccggtgcaat cggtcggtgc
      901 cgagcaatgc accgtgcagt cgcgcagctc gggttgatag cagcccgcga tggcgacgag
      961 gtgggcaacc accacaccga cgagtttgcc gctcgacacg tggtgcgcgg gtgcagcgtc
     1021 gatgccagcg gtgcgcgatg cgctacgcgg tgatagcgcg caccggcgag caggattgct
     1081 acatcgtgcg gatcagcgcg cgatccaacc gccagcgagc acgcgatcgc ctgcgtagaa
     1141 gaccgcggct tgtcccggag cggcgaccgt cggtgccgac aatcgcaccg tcgcatgatc
     1201 accctcgacg gtgacatcgg cgctgatcgg cgtaccgcga tgacggacct ggacctgtgc
     1261 cgtgagagcg gtctgcggtg ccgacagcca gcgcagatcg cggaccgcga gctcgtgctt
     1321 ctcggcagcc tcgcggggac cgacgcgaac ctcggcgcgc gccggatcga tcgcggtgac
     1381 aaatagcttg tccttcgtgg tgaggttgcc aagcccgcga tgctggccga cggtgaactt
     1441 gtgtacgcct tcgtgctggc caacgacgcg gccgtcctcg tggacgatcg tgccggtggt
     1501 cggcagcgtg cggcctcgac gcagcgcggt cgccgcgaca aagccggcgt agtcgccatc
     1561 gggaacgaaa caaatctcct gcgactcggg cttcaccgcg ttcggcagcc cgagccgcag
     1621 cgcatgctcg cggacctcgt ccttcgtcat gccgccgagt gggaagcgca ccgaggtcag
     1681 ctcgtgaccc ggcatcgaga acaagaagta cgactgatcc ttgccggcat cgaggccgcg
     1741 gtagagctgg ccgtcgatga tctgcgcgta atgcccggtg acgagcacgt cggcgccgat
     1801 cgcgcgtgca cgctgcagca gcggcgtgaa cttgatgtgc tggttgcact tcacgcacgg
     1861 attcggggtc tcgccagcga gatacgcctc gacaaaatct tcgaccacgg cgtcgccgaa
     1921 ctcggcggcg agatcgatca cgtagtgcgg aatgccgagg tgcgcgcaga ccgcgcgggc
     1981 gtcttcaatg tcgcgcgggc cacagcagcg gccacccgac gaggcagccg tgcctcgcgc
     2041 gtcgtacagc cgcatcgtca tgccgacgac gcggtgcccc tgctcgacga gcagcgcggc
     2101 agcggcagcg ctgtccacgc cgcccgacag cgcggcgacg acaagtgggg actggctcga
     2161 catgggcgcg acaccttagc agagtgcgct agtctggcac cgtgaataag tgccaggtca
     2221 cggctgcgtc gctcgacgag accggccacg gcgtcggcct tgccgagggc attcgcgtgc
     2281 atgtcgcgga tctgttgccc ggcgagcgcg ccgaggtcgc gatcgaccat gcgagcccgc
     2341 acaagcccga ggcctgggga cgcatcgtgc gccggctcgg cccgacgagt gaccagcgga
     2401 ttccgcccgc gtgcccgggc tttggccgct gtggcggctg cttgtggcag cacctgtcgt
     2461 atccatcgca gctcgaggcc aagcgtgctc gcgtggtagc ggcgctcggc ccggacgcga
     2521 aggtcggtga cgtgcggccg tcgccgcagc tgttcgggta tcgcaacaag ggcaagtacg
     2581 tggtcggcaa gagcggtgat cacgtgctgc tcggcgccta cgcgccgcgc agccaccacg
     2641 tgatcgacac gctcggctgt cgcgtggtcg cgccgatcat cgacgaggtc gcgacgtggg
     2701 tgcgtggcgc cgccgagcgc gcgcaactgg tgccgtacga cgaggcgacg cgtgtcggcg
     2761 agctccgcta cgtcgtgatc cgcgaggccg cgggcgatgt gatgatcgcg ttgatcgtcg
     2821 cgccgaacac gccgcgcgcg aagctcgagc acgtcgcgaa tgcactcgcg aagcatccgg
     2881 cggtgcgtag cctcgtcgcg atcacgaatg atcggcgcga cggcgcgatc gtgccatccg
     2941 gatcgagcgc gcaggtcctg ttcggccacg gccacctcgt cgaggagctc gccggagtga
     3001 agctcgaggt cggcgcgtcc gagttcctgc aagtcaatcg cgcgcaggcg caggcgatgt
     3061 acacgcgagt cgccgagctc gcgggtgacg tgcgcggcaa gcgtgccatc gatctgttcg
     3121 cgggcctcgg cggattcggt ctgcacctcg cgcgcgcggg cgcggaagtg atcgcggtcg
     3181 agatcgatcg tgatgcgatc tcgcagctgc ggcgcgccgc cgagcgcgca ggactgccgc
     3241 tgacgacgat tgcgggtgat gcaagtgcgg acctggcgcc acagctcggc aggccagatg
     3301 tcgtcgtcgt caatccaccg cgcaagggcc tctcggagtc gacgcgcgag ctcctcgcaa
     3361 cgatcgcccc tgcaacgatc atctacatca gttgtggacc cgagtcgttg ggacgtgatc
     3421 ttgatgcact tcgagtgcag ggctacgcac ccgacgcgat cgagccgttc gatctcatgc
     3481 ccggcaccgc gcaggtcgag acgatcgtgc ggctgcgccg aagttagtgc ttgcgtcgat
     3541 cacacgcgcg gcagtacgtc gtgagcgtga cctctcgcga tagcgacgcg tggatgaacg
     3601 ggccggaccc gcacgcgcgt ccgctgctca ccgacctcga gcgctacacc ggagagcact
     3661 accggccgca gatggctgag cctcgccacg ccgaggtgtc gctggcgaag gtcgcggtac
     3721 ctgcagcgat cggtattttg atcgtgctcg cgctgttgct cgtgaaccgc tagcggtcag
     3781 aacaagcagg tgttcgggac tgcggtgctc gtgccgagga tctgcccgac ctgatcgagc
     3841 tcgcgctgcc agtagcacag cgtgtcggcc atgtagagat atccgaactg atagaacgtg
     3901 tggttcgcgg tcttgtcgac gagcctgcgg ccgtggttgt cgtgcagatc ggcgtggcgc
     3961 gatgcgacga gcttgcgggc gcgcgcgagc agctccttcg ccttctcgta gtcgggcgcg
     4021 aagttctggt ttcgcacgaa agcgagcacc gcgttgtagg tggcgttgac gaaccgaacg
     4081 cgcagccgat cgatcgcgac gccgtcgcgg agctcgctgc tccatcgact gtcgggcaga
     4141 tcgagtgcat cgagctcgga ctcgagccca tcgagggttg tcgcataggc gtcgagatcg
     4201 gcgagcacga tgatcagcgc agcctcgagc tggcgattcg cgaccaggtc ctcgaatgtg
     4261 atccgatccg gctgcgagat gatcggcggg tcgagccggt cgccggcgtc gatcgcgata
     4321 tcgcggccga cgagatagcc gatcaggcgc tggtcgatca gtgccttctt ctgcgcggcc
     4381 atgagccggc cgacgaagtg accggcggca acgccgagat ccggcgcgta tgcatcggtg
     4441 agcagatctt ccgccgagct gccctgctcg tagctcgccc gcagggagac gacgtcgttg
     4501 agccagtagc cccattccca gccggtggag aacagcaggt gctcgtcgag cggcacgcag
     4561 cc
//
LOCUS       DASUFX010000397        13137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_203870,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000397 DASUFX010000000
VERSION     DASUFX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203870"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..251)
                     /locus_tag="VFV99_15820"
     tRNA            complement(176..251)
                     /locus_tag="VFV99_15820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Ala,seq:ggc)
     gene            complement(290..976)
                     /locus_tag="VFV99_15825"
     CDS             complement(290..976)
                     /locus_tag="VFV99_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060836.1"
                     /translation="MARQRRGGGFRPPELRGTLGTLLRTTLAQAGTLRDVLERGAREG
                     RSRLDSALSGRRRTDALAELGDIVLDLIRRGEIDLGELPEVRDIVAHLDEIDAGHVDE
                     HDESPPTPPGRKRFDARGRDEDDGTVSSRSYASARPAKPAPRVWRPPADTDRPDDEIT
                     ARGRSAPRRSGEDTVPGKTRKAAAIPNIPAKAKDPARKGGIHFDDDEDLSEYMHPDDV
                     PPKGSGDGDP"
     gene            1004..2989
                     /locus_tag="VFV99_15830"
     CDS             1004..2989
                     /locus_tag="VFV99_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060837.1"
                     /translation="MTRRLWFGVGLTVMLAACPPPEPPVKPLPPVSGGKVRVRVFTEP
                     APVKTVGAAGRFLFVATEDALQRWDDKGSVMAMTAETGLSGEHVVAMTTDADRKWMWI
                     LTDGGLGHYDASVEVYTETVPPPAPLGIDYAAIAKEGVASLAPATEGGVWLGTSKGLF
                     FVSEQGGWVATPIKDPIRALVRDRGGWLWIAATTGLVARKPNGDLIKIGVAQGCDVTQ
                     PRLLVEAPGDRVMVIGGDDQGKERLAIGKQTAWRSYRALPDHTWEAATLRGNAIVVMG
                     GGHIYRIAPASAAVRPLAREGVRLVPMTGPTTSDWVIDPTPLVVPPGATVLGAIEDQL
                     LIGTKDLGTARYRDGDAQPFTWLRRKQMFQDATDLSVACARAQDCWLATGARQAWHWN
                     GERFTAGGPDVVVLAVARDPAGPIYALHRAPQEKELHLSRIEGTTWTPVSKVSLTTPG
                     DVPEVSFARFATSGTLWVGLRYRDGEERRAYGIAIIDVVNAKVAYHRTEAVPSVPDKK
                     KEKMLPIPIGVVDADVRGDTAWFATNEGIARLSGGQVKVWTEADGLRSELARAVTIAP
                     EGGVIVATGAGAGVWDGKEWGFPAALRFEINDVVATRNGHVWMATERGIAAWDGKKVR
                     RVDTRRGLAENSVLDVAVDQFDRVWARGPGSLTLISQ"
     gene            3089..3829
                     /locus_tag="VFV99_15835"
     CDS             3089..3829
                     /locus_tag="VFV99_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060838.1"
                     /translation="MTNRTILIGLAFALATMACGKKQDDKEAACSRVAAKIRDEELAK
                     CAGDKDCQHEAEEMFQGRKDICKMAAKANEGGKPKSLNEQADDAEKKCDKGDAESCAT
                     IGGAYLLGKGGKEKDEAKGLALVKKACDGKSAFGCEIYGRALDEGKGIAPDKEGAKSY
                     FKQSCDLGGAGGCRALALQMETTDPARIPLLQKACDKDDKLGCMGLGAAYLHGNQGAP
                     KDLAKAKQYLQKSCDLGATSACDKAHEL"
     gene            complement(3837..4346)
                     /locus_tag="VFV99_15840"
     CDS             complement(3837..4346)
                     /locus_tag="VFV99_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060839.1"
                     /translation="MRAAIVFACLASNALTAHADSFEDHDTFGLDVSTAWEPANGDAF
                     GAGPMFRFETFTSRMPDWLGLVSRFGVIVDSADRVYSPVAIGLEVRHDWFYYTVEGGA
                     TLASRDSHMDDALYFDWTFGAAAGVRLGHWDLRANVMLGGLFDKAVWMFSVGRDFVRI
                     DSKITRTTL"
     gene            complement(4452..6815)
                     /locus_tag="VFV99_15845"
     CDS             complement(4452..6815)
                     /locus_tag="VFV99_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5060840.1"
                     /translation="MRGIICFVVATLPALGACGDDPSTTITCGDGTEGALTATAPLTV
                     TADGAKDLRGAAIAAGPGTTIPSTPLTIKCAVDIVPAGYVALGPAVTFGADGTWSDRP
                     FFLTLPYKAARLPKDAERRHVRIVAKRDGQTDPFFAPVSNRKYEDDDRYASRVTFKAG
                     ELTTYQVVAELTAGTKETQQFGWRAIVGISMGGNAAMSIALRHPDKFDIFADLGGEPG
                     PSMVYSLSMVSDFLFGGFCTVDDEAAGRGNVGQLCPNKSTRKDQFEIEADYEHMITQD
                     GDGVGLTLKRNLYMKASRDLARALSNPALYNPMNPYVPPGVSPDFIEVDAATRCANPT
                     KLDDFYDREFNPTGSHPVITFCDGGDGPALGLAVFDPNLPQTNPAELLLAVDLNNNGK
                     RDSGEPVITNAYEPFSDVGTDSLADKDEPGYDPVTNPDPNRDDYHSLRNPLGTEKNGN
                     YESGEPFVDAGLDGVMGTCQAGQTPPSGTAGCYDFGEGNSKWDLSPNVARWYESDVIG
                     RLDALDAEQRRHMSMWFDAGIRDFLNASVSANAGVGQAMAKYSLPFGVYDNFSILDGG
                     DSELAYDFEDIDWTNIPKNGYLRYGDPDATPDQIKNGDGRHVGTANQIIYRARTAFAW
                     INRRWPDGDLSDTYDGGQIIKDLMFTSPTTGRVNPFGVFLPPGYNKPENATKTYPVVY
                     FLHGYGQEPKDLVDLSSVFALNMIATNAIETRFQKYIIVYVDGRCRPNIDGTPVDPTG
                     DRCEGGTFYLDAPLGGLARMETNMLDLMDYIDANYRTKPASSAQVVD"
     gene            6925..7251
                     /locus_tag="VFV99_15850"
     CDS             6925..7251
                     /locus_tag="VFV99_15850"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5060841.1"
                     /translation="MKQLADMHVAPPLRRWSTRLGLALVVAIAIGYVPGQLMRRDPRE
                     QKLTEQLEAVRAESRELAARNMVLYRDVNALRTDVRAIEDRARGDLGMVYPDEVILRV
                     RRGSTP"
     gene            7320..9998
                     /locus_tag="VFV99_15855"
     CDS             7320..9998
                     /locus_tag="VFV99_15855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5060842.1"
                     /translation="MKAKASATTDVFARHDSARVSKGDPEEIADEDTAPTKRPTATED
                     VSTKTTAHAEAVPKRATSLESAPIGLRKASITDSGGAKLRGGDFGDLAKPRDSGPVPK
                     SGRIAHGTGGAEALSAKLKTGPGDSRRFERVDLGKRKPDAVIAAKHVKTGPITIKLQA
                     RWKRALRRAGIVSVGAVAAAFACVVVAIGGVDGASLFAAQMPLPQTMIATSIAALVLV
                     AVIRRVQFSPPSARAQRVSMWLAAAQDLADLELCLALVAGTHVVIAVTGGLESHAYPV
                     LYGLVAFAVTVLARPGAFATIGAALFLEAALLVRSGIDDTTILAASLHALFLAGAGLA
                     HALLLRGMTSRYRQRRARRLEEELAALRDSARDYRLIAAALGPGSRAPRPREEEERLL
                     AIGGVGMIGDAMSWVLTTLKRSLNARTVALLWVEDSDGEGVKLTEVASDADDITEAPR
                     LPSAGVLGAVIRDRAPLLVAATKPGQLPYYDNGRAGVALVAVPVMEGTHLRGILAADR
                     DLPFSEADRDLLADAGGQVLRVVQAEQVFRAVERAKYEHERFYQATAMLGRALTPEQV
                     MDTAFDACAAIVEYDAAAIALYDKDRAKHRIAAIRIGRDGAGIIDPSLPGFEFKDNAG
                     LASMVIKNRHYLPAGGEPREVSAPIYTRKIKIDDANSLLVLPLLSADEAIGTFTLIAR
                     AERRFGKDVREMLAVIANQVAVSLQNGYLYKQMETMATTDGLTGLTNHRTFQSRFEDL
                     LQRAQRHNHKVALLLCDVDHFKKVNDTYGHPIGDEVLRRVAKVLQEVPRKIDIPARYG
                     GEEFAVILDGVDVTQAKAVAERIRIEISKVVVETEKGPLSVTESIGVASFPDDGRDRA
                     TLIERADLALYQAKHSGRNRVVTWSEAQAAKAKKAG"
     gene            complement(10045..10212)
                     /locus_tag="VFV99_15860"
     CDS             complement(10045..10212)
                     /locus_tag="VFV99_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060843.1"
                     /translation="MKKLSTMILVVAVATATACFGGKRGGRGTNVGTGSDDWPPGQGG
                     QSPALTGSGHP"
     gene            10383..12530
                     /locus_tag="VFV99_15865"
     CDS             10383..12530
                     /locus_tag="VFV99_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060844.1"
                     /translation="MPREPAEVGSAANSYEILAKLAMGGMAEIFLARGASAAGVERYV
                     VLKRVLRHRADDVHFVQMFLDEARLAAQLQHPNIAQVYDIGKLGDSYFFTMEYVHGET
                     VRTLLSRSHALRRQIPIGSVLSIIAGAAAGLHHAHERKGFDGRHLGIVHRDVSPSNLM
                     VSYEGTVKVVDFGVAKAAHRSTETVSGTVKGKISYMSPEQCKGAAEIDRRSDLFSLGI
                     VMWEVLTTEKLFRRNSDFETMQSIVSDDVPPPSHYRPEIPPEIDHIVTSLLSKDPARR
                     YQTADELHEAIENVAVRTGSVLSAASLGRYMRELFGQRPEPWIELQTAEQHPEAFTVT
                     SEPIPNNLAMSKADDFDRKLSAVPALTQRFTVPEGQARSPSAQPTQPLRVGADLVTTL
                     PMGADPQDLMKTMPQRESRVRNVVKLPLEPPNRPSTPIPAPLAPSGPMMAPQYGSDSG
                     ISRPHSPSLTPGSGSSPQYPIASGVANPSFSAIPQNYPVAYQQVPEKRRSRVLMIMLP
                     AAIVGIVIGIAFGLGGGEKKKLETAQPEQPTAGTTTMVATPEPGTNPAPGVRVIDSQP
                     TVPDKPKSVEPGEPIKSVDMTKPEKPKSEDKPKSDGKPKSDDKPKSDDKPKSDDKPKT
                     PDVAALFKAANYGDVVTACSSSTKLVALNAVSCTVAACKTKQAAKAKRWLSQVGPAKK
                     ASVVHDCDGVIPAPEKGQPPSDPCKTDPMACQH"
     gene            12554..>13137
                     /locus_tag="VFV99_15870"
     CDS             12554..>13137
                     /locus_tag="VFV99_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060845.1"
                     /translation="MVRVLVAALVVGLASIAAADGESTKLFEEGRELAKDGKYEEACA
                     KFARSLELDRATGTLVNYADCHEHLGHLAQAWRLFDEAARASEKESNPERAKFARDRA
                     QALVPKTSTIVVKLAAADTPGLAVSIAGRSAKPAPEIREVVDPGEVTIEVTATNATPF
                     TKTERAEAGLKIVVEVPALAEPHEGGGGGTTTERR"
BASE COUNT         2291 a         4176 c         4572 g         2098 t
ORIGIN      
        1 ggtcttgcgc gcccacagcc agttgaagag ggcagtgcgc gcgccgccga tatggagata
       61 gccggttgga gaaggagcga accgaagacg tggcttccgc atgggcctcg caacggattc
      121 ggatgctccg acccgaaatc ccgaaaatgc ggccgctcag tgcggccggg ttacctggtg
      181 gagataaggg ggatcgaacc cctgacctcg tgaatgccat tcacgcgctc tcccagctga
      241 gctatatccc caggggtgtg cgggtttcta ccggcgccct accggcatgt caagggtcgc
      301 cgtcccccga gcccttgggc gggacgtcgt ccggatgcat gtattcggac agatcctcgt
      361 cgtcgtcgaa gtgaatcccg cccttgcggg ccgggtcctt cgccttggct ggaatgttgg
      421 ggatcgcggc agctttgcgg gtcttgccgg gcacggtgtc ttcgcccgag cggcgcgggg
      481 cggaccggcc gcgcgcggtg atctcgtcgt ccggacgatc ggtgtcggcc ggcgggcgcc
      541 agacccgagg ggcgggcttg gccgggcgtg cgctggcgta cgagcgggat gagacggttc
      601 cgtcgtcttc gtcacggccg cgtgcgtcga agcgctttcg gccgggcgga gtcggcggcg
      661 attcgtcatg ctcgtcgacg tgaccggcgt cgatctcgtc gagatgcgcg acgatgtcgc
      721 ggacttcggg cagctcgccg agatcgattt cgccgcggcg gatgagatcg agaacgatgt
      781 cgccgagctc ggcgagcgcg tcggtgcgcc ggcggccgga gagtgcggag tcgagccgac
      841 tgcgaccttc tcgtgcgccg cgctcgagca cgtcgcggag cgtgccggcc tgggcgagcg
      901 tcgtgcgaag cagcgtgccg agcgtgccgc gcagctcggg agggcggaag ccaccacctc
      961 gtcgttgccg tgccatgcgc ggaggttata ccgtcgatgg gcagtgacgc gacggctttg
     1021 gttcggggtc ggcctgacgg tgatgctggc tgcatgccca ccgcctgaac cacccgtcaa
     1081 gccgttgccg cccgtgtcgg gtgggaaggt tcgcgtgcgc gtgttcaccg agccggcgcc
     1141 cgtgaagacg gttggcgcgg ccgggcggtt cttgtttgtc gcgacggaag atgcgttgca
     1201 gcggtgggac gacaagggct cggtgatggc aatgaccgcc gagactgggt tgtcgggcga
     1261 gcacgtcgtc gcgatgacga ccgatgctga ccgcaagtgg atgtggattc tgaccgacgg
     1321 cggattgggg cactacgacg cgagcgtcga ggtttatacc gagacggtgc caccgccggc
     1381 gccgctcggc atcgactacg ccgcgatcgc gaaggaaggc gtcgcgagct tggcacctgc
     1441 gacggaaggt ggcgtgtggc tcggcacgtc gaagggtttg ttcttcgtga gcgagcaggg
     1501 cggctgggtt gcgacaccga tcaaggatcc gattcgcgcg ctcgtacgag atcgtggtgg
     1561 gtggttgtgg atcgcggcga cgaccggatt ggtcgcgcgc aaaccgaacg gcgacttgat
     1621 caagatcggc gtcgcgcagg gatgcgacgt gacgcagccg cgcttgctcg tcgaagcgcc
     1681 cggcgatcgc gtgatggtga tcggcggcga cgatcagggc aaggagcggc tcgcgatcgg
     1741 caagcagacg gcgtggcgca gctatcgcgc gctgcccgac cacacgtggg aagcggcgac
     1801 gttgcgcggc aatgcgatcg tcgtgatggg cggtggccac atctatcgca tcgcgcccgc
     1861 gagcgcggcg gtgcggccgt tggcacgcga aggtgtgcgg ctcgtgccga tgaccggccc
     1921 gacgacgagc gactgggtga tcgatccgac gccgctcgtc gtgccgcctg gcgcgaccgt
     1981 gctcggcgcg atcgaagatc aattgttgat cggcacgaag gatctgggca ccgcgcgcta
     2041 tcgcgacggc gatgcgcaac cgttcacgtg gctacggcgc aagcagatgt ttcaggacgc
     2101 gacggacctg agcgtcgcgt gtgcgcgagc gcaggattgt tggctcgcga ccggtgcacg
     2161 ccaagcgtgg cactggaacg gcgagcgatt cacggcaggc gggccggatg tcgtcgtgct
     2221 cgccgtcgcc cgcgatccgg cgggaccgat ctatgcgctg catcgcgcgc cgcaggagaa
     2281 agagctgcat ctgtcgcgca tcgagggcac gacgtggacg ccggtgtcga aggtctcgct
     2341 cacgacgccg ggcgatgtac ccgaagtcag cttcgcgcgc ttcgccacgt cgggcacgct
     2401 gtgggtcggc ttgcggtatc gcgatggcga agagcgccgc gcgtacggca tcgcgatcat
     2461 cgatgtcgtc aacgcgaagg tcgcgtatca ccgcaccgag gcggtgccgt cggtgcccga
     2521 caagaagaag gaaaagatgc tgccgattcc gatcggcgtc gtcgatgccg acgtgcgcgg
     2581 cgacaccgcg tggtttgcga cgaacgaagg catcgcgcgc ttgtcgggcg ggcaagtgaa
     2641 ggtgtggacc gaagcggatg gtttgcgcag cgagctcgcg cgcgcggtga cgatcgcgcc
     2701 ggagggcggt gtgatcgtcg cgaccggcgc gggcgcaggt gtgtgggacg gcaaggagtg
     2761 gggcttcccg gcggcgctgc gcttcgagat caatgatgtc gttgcgacgc gcaacggcca
     2821 cgtgtggatg gcgaccgagc gcggcatcgc ggcgtgggac ggcaagaagg tgcggcgtgt
     2881 cgatacgcgg cgcgggctcg ccgagaacag cgtgctcgac gttgccgtcg atcagttcga
     2941 tcgcgtgtgg gcgcgcgggc ccggcagcct gacgctcatc tctcaataat agaggtgcac
     3001 ctgcgctgca cccggtgcac gcgaacgcgg gcgcgtgggg tatccagcac ttgcgcttgg
     3061 catgtatggt gctgagtcgg gggcaaccat gaccaaccgt acgatcctga tcggcctggc
     3121 gttcgcgctc gcgacgatgg cctgcggcaa gaagcaagat gacaaggaag ccgcgtgtag
     3181 ccgcgtggcc gcgaagattc gcgacgaaga gctcgcgaag tgtgccggcg acaaggactg
     3241 ccagcacgaa gcggaagaga tgtttcaggg tcgcaaggac atctgcaaga tggccgcgaa
     3301 ggcgaacgaa ggcggcaagc cgaagtcgct gaacgagcag gccgacgacg ccgagaagaa
     3361 gtgcgacaag ggcgacgccg agtcgtgcgc gacgatcggc ggtgcgtact tgctcggcaa
     3421 gggcggcaaa gagaaggacg aagcgaaggg cctcgcgctc gtgaagaagg cgtgcgacgg
     3481 caagagcgcg ttcggttgcg agatctacgg ccgcgcactc gacgagggca aaggcatcgc
     3541 gccggacaag gaaggcgcga agtcgtactt caagcagtcg tgcgacctcg gtggtgccgg
     3601 tggttgtcgc gcgctcgcgc tgcagatgga gaccaccgat ccggcgcgca tcccgctgct
     3661 ccagaaagcg tgcgacaagg atgacaagct cggctgcatg ggcctcggcg ccgcgtacct
     3721 gcacggcaac cagggcgcgc cgaaagatct cgcgaaggcg aagcagtacc tgcagaagtc
     3781 gtgtgacctc ggcgcgacga gcgcgtgcga caaagcgcac gagctgtaga gcactcctag
     3841 agtgtcgtgc gcgtgatctt cgaatcgatg cgcacgaagt cgcggccgac cgagaacatc
     3901 cagacggctt tgtcgaacag accgccgagc atgacgttcg cgcgcaggtc ccagtgaccg
     3961 agtcgtacgc cggccgcggc gccgaacgtc caatcgaaat agagggcgtc gtccatgtga
     4021 ctgtcgcggc tcgctaacgt cgcgccacct tcgaccgtgt aatagaacca atcgtgacgg
     4081 acctcgaggc cgatcgctac aggcgagtac actcgatcgg cgctgtccac gatcacaccg
     4141 aagcgcgata cgaggccgag ccagtccggc attcgcgacg tgaacgtctc gaagcggaac
     4201 atcggtccgg cgccgaacgc gtcgccgttc gcgggttccc atgcggtcga gacgtcgaga
     4261 ccgaacgtgt catggtcctc gaagctgtcg gcgtgcgcag ttagtgcatt tgaagcgaga
     4321 caagcaaaga cgatggcagc gcgcatgctc ctctttcacg gcggcgcgct ccatcgtgca
     4381 gtgcgtgtgc cgcacgcagt gccgcgcaca tagcgcgcga acgtgaagca acggctacag
     4441 cgaagtgagc ttcagtcgac gacttgcgcc gagctcgctg gcttcgtccg gtagttcgcg
     4501 tcgatgtagt ccatgaggtc gagcatgttc gtttccatgc gcgcgagccc accgagcggc
     4561 gcgtcgagat agaacgtgcc gccctcgcag cgatcgcctg tcggatcgac cggcgtgccg
     4621 tcgatgttcg gacggcagcg gccgtcgacg tacacgatga tgtacttctg gaatcgcgtc
     4681 tcgatcgcgt ttgtcgcgat catgttgagc gcgaacacgc tcgacagatc gacgaggtcc
     4741 ttcggctcct gcccgtagcc gtgcaggaag tagacgaccg gatacgtctt cgtcgcgttc
     4801 tcgggcttgt tgtagccggg tggcaggaac acgccgaacg gattcacgcg gcccgtcgtc
     4861 ggcgacgtga acatgaggtc tttgatgatc tggccgccgt cgtaggtgtc gctgagatcg
     4921 ccgtcgggcc agcgccggtt gatccacgca aacgccgtac gcgcgcgata gatgatctgg
     4981 ttcgctgtac cgacgtggcg cccgtcgccg ttcttgatct gatcgggcgt cgcgtcggga
     5041 tcgccgtagc gcagatagcc gttcttcgga atgttcgtcc agtcgatgtc ttcgaagtcg
     5101 taggcgagct cgctgtcgcc gccgtcgagg atcgagaagt tgtcgtagac accgaacggc
     5161 aggctgtact tcgccatcgc ttgaccgaca cccgcgttcg ccgacaccga cgcattgagg
     5221 aagtcgcgaa tgccggcgtc gaaccacatg ctcatgtggc ggcgctgttc agcatcgagc
     5281 gcgtcgaggc gcccgatgac gtcgctctcg taccagcgcg cgacgttcgg cgacaggtcc
     5341 cacttgctgt tgccttcccc gaagtcgtag caaccggcgg tgccgctcgg cggagtctgg
     5401 ccagcctgac aggtgcccat cacgccatcg agaccggcgt cgacgaacgg ctcgccgctc
     5461 tcgtagttgc cgttcttctc ggtcccgagc gggttgcgca gcgagtggta gtcgtcgcgg
     5521 ttcggatcgg gattcgtcac cgggtcgtag ccgggctcgt ctttatcggc gaggctgtcg
     5581 gtgccgacgt cggagaacgg ctcgtacgcg ttcgtgatca cgggctcgcc gctgtcgcgc
     5641 ttgccgttgt tgttgagatc gacggcgagc aacagctcgg cgggatttgt ctgcggcagg
     5701 ttcgggtcga acaccgcgag gccgagtgcg ggaccgtcgc cgccgtcgca gaacgtgatc
     5761 accgggtggc tgcccgtcgg gttgaactcg cgatcgtaga agtcgtcgag cttcgtcggg
     5821 ttcgcgcaac gcgtcgccgc gtcgacctcg atgaaatccg gtgacacacc gggcggcacg
     5881 tacgggttca tcgggttgta gagcgccggg ttcgacagcg cgcgtgcgag atcgcgcgac
     5941 gccttcatat agaggttgcg cttgagcgtg aggccgacgc catcaccgtc ctgcgtgatc
     6001 atgtgctcgt agtcggcttc gatctcgaac tggtctttgc gcgtcgactt gttcgggcag
     6061 agctgaccga cgttgccgcg accggccgct tcatcgtcga cggtacagaa cccgccgaac
     6121 aagaagtcgc tgaccatcga gagcgaatag accatcgacg ggccgggctc accgccgagg
     6181 tccgcgaaga tgtcgaactt gtccgggtga cgtagcgcga tcgacatcgc cgcgttaccg
     6241 cccatcgaga tgccgacgat cgcgcgccat ccgaactgct gcgtctcctt cgtgccagcc
     6301 gtcaattcag cgaccacctg atatgtcgtg agctcgccgg ccttgaacgt gacgcgcgac
     6361 gcgtagcgat cgtcgtcctc gtacttgcga ttcgataccg gcgcgaagaa cgggtccgtc
     6421 tggccatcgc gcttcgcgac gattcgcacg tggcgccgtt cggcgtcctt cggtaggcgc
     6481 gccgccttgt atggcagcgt caagaaaaac gggcgatcgg accatgtgcc atcggcaccg
     6541 aacgtgaccg cggggccgag cgcgacgtag ccggccggca cgatgtcgac cgcacatttg
     6601 atcgtgagcg gtgtgctcgg aatcgtggtg ccgggccccg ccgcgatcgc cgcgccgcgc
     6661 agatccttcg cgccgtccgc ggtgaccgtg agcggcgcag tcgccgtcaa cgcgccttcg
     6721 gtgccatcac cacaggtgat ggtggtgctc gggtcgtcac cgcatgcgcc aagcgcaggc
     6781 agcgtggcga caacgaaaca gatgatcccc ctcatgtgcg cgcagcgtac acataggccg
     6841 tcgaaactcc atcagaaagt gatcccccgt gacgttggcc aaaagttggc ccccgaaagc
     6901 cgcgatccgt aacgtcgggg gacggtgaag caactggccg acatgcacgt ggcaccgccg
     6961 ctacgtcggt ggtcgacgcg gctcggactc gcgctcgtgg tcgcgatcgc gatcggctac
     7021 gtgccaggcc agctgatgcg ccgcgacccg cgcgaacaga agctcaccga acaactcgag
     7081 gccgtacgcg ccgaatctcg cgagcttgcc gcgcggaaca tggtgctcta ccgcgatgtc
     7141 aatgcactgc ggaccgacgt ccgcgcgatc gaggatcgtg cgcgcggcga cctgggcatg
     7201 gtctatcccg atgaagtcat cctgcgcgtc cggcggggga gcacgccatg agcgcgccaa
     7261 ggaagcccgc gaaaggcacg cggagtgagc cggcgcgccg cgcaaccgac agtcgcccgg
     7321 tgaaggcgaa agcgtcggcg acgaccgacg tgttcgcgcg tcacgactcg gcgcgcgtca
     7381 gcaagggcga ccccgaggag atcgccgacg aagacaccgc gccgacgaag cggccgaccg
     7441 cgaccgagga cgtgtcgacg aagacgaccg cacacgcgga agcggtgccg aagcgcgcga
     7501 cgtcgctcga gagcgcaccg atcggtctgc gcaaggcgag catcaccgat agcggcggcg
     7561 cgaagctgcg cggcggcgac ttcggcgacc tcgcgaagcc gcgcgacagc ggcccggttc
     7621 cgaagagcgg ccggatcgca catggcaccg gcggcgccga ggcgctgagc gccaagctca
     7681 agaccggccc cggtgactcg cgtcgcttcg agcgcgtcga tctgggtaag cgcaagccgg
     7741 atgcagtgat cgcggcgaag cacgtgaaga ccggcccgat cacgatcaag ctgcaagctc
     7801 gctggaagcg cgcgctccgt cgcgccggca ttgtctcggt cggcgccgtc gccgctgcgt
     7861 tcgcgtgcgt ggtcgtcgcg atcggcggtg tcgatggcgc gtctctgttc gctgcgcaga
     7921 tgccgctgcc gcagacgatg atcgcgacgt cgatcgcggc gctcgttctc gtcgccgtga
     7981 ttcgccgcgt ccagttctcg ccgccgagtg cgcgcgcgca gcgggtgtcg atgtggctcg
     8041 cggccgcgca ggatctcgcc gatctcgagc tctgccttgc cctcgtcgcc ggcacgcacg
     8101 tcgtgatcgc agtgactggc ggcctcgagt cgcacgcgta cccggtgctc tacggactcg
     8161 tcgcgttcgc ggtcacggtg ctcgcccgac ccggtgcgtt cgccacgatc ggcgccgcat
     8221 tgttcctcga agcggcgctg ctcgtgcgct ccggcatcga cgacaccacc atcctggccg
     8281 cgtcactgca cgcactgttt ctcgccggcg ccgggctcgc gcacgcactg ctgcttcgcg
     8341 ggatgacctc gcgttatcgc caacgtcgag ctcgccggct cgaagaagag ctcgccgcgc
     8401 tacgcgactc cgcgcgtgac taccgattga tcgccgccgc gctcggtccg ggtagccgtg
     8461 caccgcgtcc gcgcgaggaa gaagagcgcc tgctcgcgat cggcggtgtc ggcatgatcg
     8521 gcgacgcgat gagctgggta ctcaccacgc tgaagcgctc actcaacgcg cgcaccgtcg
     8581 cgctgctctg ggtcgaagac agcgatggcg aaggcgtcaa actcaccgag gtcgcgagcg
     8641 acgccgacga catcaccgaa gcgccgcggc tgccgagcgc tggcgtgctc ggtgcggtga
     8701 ttcgcgatcg cgctccgctg ctcgtcgcgg cgacgaagcc gggccagctt ccgtattacg
     8761 acaacggtcg tgccggcgtc gcgctcgtcg cagtgccggt catggaaggc acccacctgc
     8821 gcggcatcct cgccgccgat cgcgacttgc cgttcagcga agccgatcgc gacctgctcg
     8881 ccgacgccgg tggtcaagtg ctgcgcgtcg tccaggccga gcaagtgttc cgcgccgtcg
     8941 agcgtgcgaa gtacgagcac gagcggttct atcaagcgac cgcaatgctc ggccgcgcgc
     9001 tcacgcccga gcaggtgatg gacaccgcgt tcgacgcctg cgccgcgatc gtcgagtacg
     9061 acgccgctgc gatcgcgctg tacgacaagg atcgcgcgaa gcaccgcatc gccgcgattc
     9121 gcatcggtcg cgacggtgcc ggcatcatcg atccgtcgct gcccggcttc gagttcaagg
     9181 acaacgctgg ccttgcgtcg atggtgatca agaaccgcca ttacctgccg gcgggcggcg
     9241 agccgcgcga ggtgtcggcg ccgatctaca cgcgcaagat caagatcgac gacgcgaact
     9301 ctctcctcgt gctgcctctg ctctccgccg acgaggcgat cggcacgttc acgctgatcg
     9361 cgcgcgccga gcggcgcttc ggcaaggacg tgcgcgagat gctcgcggtg atcgcgaacc
     9421 aggtcgcggt gtcgctgcag aacggctacc tctacaagca gatggagacg atggcgacga
     9481 cggacggcct caccggcctg acgaaccatc gcacgttcca atcgcgcttc gaggatttgc
     9541 tgcagcgcgc gcagcgtcac aatcacaagg tcgcgctgct gctctgcgac gtcgatcatt
     9601 tcaagaaggt caacgacacc tacggtcacc cgatcggcga cgaagtgctg cgccgcgtgg
     9661 cgaaggtgct gcaggaagtg ccacgcaaga tcgacattcc ggcgcgctac ggcggcgaag
     9721 aattcgcagt catcctcgac ggcgtcgacg tcacacaagc gaaggcagtt gccgagcgca
     9781 ttcgcatcga gatctcgaag gtggtcgtcg agaccgagaa gggaccgcta tcggtcaccg
     9841 agtcgatcgg tgtcgcctca ttccccgacg acggccgcga ccgcgcgacg ctcatcgaac
     9901 gcgcggacct cgcgctctat caagcgaagc acagcggtcg taaccgcgtt gtcacgtgga
     9961 gcgaagcgca ggctgcgaaa gccaagaagg ccggatagcg ccagcctccg cagtgcggtt
    10021 ggcgcaatcg gtctctcgtg cgcgctacgg atgtccggac ccggtgagcg ccggggactg
    10081 gccaccttgg ccgggcggcc agtcatcgct gcccgttcca acgttggtac cgcgcccgcc
    10141 acgcttgccg ccgaaacacg cagtcgcggt cgcaaccgcg acgacgagga tcatggtcga
    10201 cagctttttc atgacgggaa gcacagcaag gcccatgctc gacgaagctc gcggaactgc
    10261 atgcgcgatc tgtgcagcgg agacggccgc gagacatcgg cgctcgtcgt gatgtaggtg
    10321 taacgacgcc agcgccgcgg accgcgcgac gcatactgat cgtggcgtac aattcacggc
    10381 aggtgcccag ggagcctgcg gaagtcggct ctgccgcgaa cagctatgaa atcctcgcga
    10441 agctcgcgat gggtgggatg gcagagatct ttctcgcacg cggcgcgagc gcagcaggtg
    10501 tcgagcgcta cgtagtgctc aagcgcgttc ttcgccatcg cgcggacgac gtgcacttcg
    10561 tgcagatgtt cctcgacgag gcacgtctcg ccgcgcagct tcagcatccg aacatcgcgc
    10621 aggtctacga catcggaaag ctcggcgact cgtacttctt cacgatggag tacgtgcacg
    10681 gcgagaccgt gcgcacgctg ctgtcgcgct cgcatgcgtt gcgtcgacag attccgatcg
    10741 gctcggtgct ctcgatcatc gcaggcgcgg ccgcgggtct tcaccacgcg cacgagcgca
    10801 aaggcttcga tggccggcat ctcggcatcg tccatcgcga cgtctcgccg tcgaacttga
    10861 tggtcagcta cgagggcacg gtgaaggtcg tcgactttgg tgtcgcgaag gctgcgcatc
    10921 gcagcaccga gacggtgtcg ggcacggtga agggcaagat ctcgtacatg tcgcccgagc
    10981 agtgcaaggg cgccgccgag atcgatcggc gcagcgactt gttctcgctc ggcatcgtga
    11041 tgtgggaagt gctgacgacg gagaagctgt tccggcgcaa cagtgacttc gagacgatgc
    11101 aatcgatcgt cagcgacgac gtaccaccac cgtcgcacta ccgccccgag attccgccgg
    11161 agatcgatca catcgtcacg agcctgttgt cgaaggatcc ggcgcggcgc taccagaccg
    11221 ccgacgagct ccatgaagcg atcgaaaacg tcgcggtacg cacgggctcc gtgctgtcgg
    11281 cggcgagcct cggccggtac atgcgcgagc tgttcggtca gcgccccgag ccctggatcg
    11341 agctccagac cgcagagcag catcccgaag cgttcacggt cacgagcgag ccgattccga
    11401 acaacctcgc gatgtcgaag gccgacgatt tcgatcgcaa gctgtcggcc gtgccggcgc
    11461 tcacgcagcg cttcaccgtg cccgaaggcc aagcacggtc accgtccgcc cagccgaccc
    11521 agccgctgcg cgttggcgcc gatctggtga cgacgctgcc gatgggggcc gatccgcagg
    11581 atctgatgaa gacgatgccg cagcgcgagt cgcgcgtgcg caacgtcgtc aagttgccgc
    11641 tcgagccgcc gaaccggccg tcgacgccga ttcccgcgcc gctcgcgccg agcggaccga
    11701 tgatggcacc gcagtacggg tccgatagcg gcatcagccg gccgcactcg ccgtcgctga
    11761 cgccaggtag tgggagcagc ccgcaatatc cgatcgcgag cggcgtggcg aatccgtcgt
    11821 tctcggcgat cccgcagaac tatccggtcg cgtatcagca ggtgccggag aagcggcggt
    11881 ctcgcgtgct catgatcatg ttgcccgcgg cgatcgtggg catcgtgatc ggcatcgcgt
    11941 tcggcttggg cggcggcgag aaaaagaagc tggagacggc gcaaccggag cagcctacgg
    12001 cgggcacgac gacgatggtc gcgacgcccg agccgggaac gaatccggcg cccggcgtgc
    12061 gggtcatcga ctcgcagccg acggttccgg acaaaccgaa gtccgtcgag ccgggcgagc
    12121 cgatcaagtc cgtcgacatg acgaagcccg agaagccgaa gtccgaggac aagccgaagt
    12181 ccgacggcaa gccgaagtcc gacgacaagc caaagtccga cgacaagcca aagtccgacg
    12241 acaagccgaa gacgccggat gtcgcggcgc tgttcaaggc ggccaactac ggcgacgtcg
    12301 tcactgcatg cagctcgtcg acgaagctcg tcgcgctcaa cgcggtctcg tgcaccgtcg
    12361 ccgcgtgcaa gacgaagcag gcggcgaagg cgaaacgttg gctctcgcaa gtcgggccgg
    12421 cgaagaaggc gtccgtcgtg cacgactgcg acggtgtgat accggcaccg gaaaagggcc
    12481 aaccgccgag tgatccgtgc aagacggatc cgatggcttg ccagcactga ggcatctgcg
    12541 tgacgatatg gccatggtgc gagtcctcgt ggctgcgctc gtcgtcggtc tggcgagcat
    12601 cgcggccgcg gacggcgaga gcacgaagct gttcgaggaa ggtcgcgagc tcgcaaagga
    12661 cggcaagtac gaagaggcgt gcgcgaagtt cgcacgcagc ctcgagctcg atcgcgccac
    12721 cggcacgctg gtcaactacg ccgactgcca tgaacacctc ggccacctcg cgcaggcgtg
    12781 gcgactgttc gacgaagcgg cgcgcgcatc cgagaaggag tccaacccgg agcgcgcgaa
    12841 gttcgcccgc gaccgcgcgc aggcgctcgt gccgaagacg agcacgatcg tcgtcaagct
    12901 ggcagccgcg gacacgcccg ggcttgcggt ttcgatcgca gggcgtagcg cgaagccggc
    12961 gcccgagatc cgcgaggtcg tcgatcccgg cgaggtcacg atcgaggtca ccgcgaccaa
    13021 cgccacgccg ttcacgaaga ccgagcgtgc ggaggcgggc ctcaagatcg tcgtagaggt
    13081 cccggcgctc gccgagcccc atgagggcgg cggcggaggg acgacgacgg agcgccg
//
LOCUS       DASUFX010000398         9408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_482076,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000398 DASUFX010000000
VERSION     DASUFX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482076"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFV99_15875"
     CDS             complement(<1..969)
                     /locus_tag="VFV99_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5060846.1"
                     /translation="MHPKGTCSPGGSLARALAFLGLAACGSPTMMQHGDDDDVAGDAA
                     LGSDSGGSDSDAQISATCDGKTAQPLDSDWSLTVGSLTRTARVHVPASYDPSKRTPLV
                     INLHGRTSNASQQAWLSHAIAKSDAAGFIVIHPEAWGSPTSWNAGGGCCDPAASNNID
                     DSGFMTAVLDEAEARLCVDPTRIFFEGLSNGGYLVHRVACELTDRVGAIGAVAGLLQL
                     PTCAPTKPMPVFLVHGTADQLVSYDWVDETVSYWVAKNHCTTTATTYQNGVATCVTHG
                     GCTDGADVVLCTMQGGGHQWPGGETVPFLGNNSNDLIATDALWDFFV"
     gene            complement(969..1904)
                     /locus_tag="VFV99_15880"
     CDS             complement(969..1904)
                     /locus_tag="VFV99_15880"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5060847.1"
                     /translation="MRAWLLVWLVACSSPTQSIDQLDAAGGGGSDMGSGSSDAPPQSA
                     TCDGKTAQPLDATWNIMVGSSMRVAKVHVPASYDPSKRTPLVVDVHGRTANASGEMSL
                     THSIAKSDAAGFIAIYPESATTPTSWNSGTCCEPASSSNLDDTGFFKKLLDEAEAKLC
                     IDTSRVYMMGMSNGAYESHRLGCEMADRIAAIGPVAGLLLFQGCSPTKPMPVMMVNGT
                     SDSLSLYQYVASGVNFWKGKNGCTTMTTTYTNGDASCVTYGGCTAGADVVLCTIQDGG
                     HQWPGGETLPFLGNKSDNLIATDALWDFFVAHPRS"
     gene            complement(1993..2949)
                     /locus_tag="VFV99_15885"
     CDS             complement(1993..2949)
                     /locus_tag="VFV99_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5060848.1"
                     /translation="MHAALSNPFMLGKKKPIVVGHRGVPARHQENSLAGFKRAVQLGV
                     PAIELDVQLTADRRAVVLHDANLRRLTGNPRNVWDLTWDQLSKLRVRRELPMGVDVHG
                     QRTVARYEREEPIPLLAEVLAEVAGKVAINIELKLNAFGFWQTEIARVVAEEVRKANA
                     EDHVILTSFDPRKLRAALSAYPKLATGFCFDDTMLDFAAPFLERLPYLRNKLSLPADG
                     HIRTNARRLLNRILETNLVGKLLGTRVVGAEHTIVGSRTVKRLHDQGIAIGTHTLFPL
                     GSTTGKPISPSAMTAQEVERLVALGVDWIESDDPERLLNLIG"
     gene            3001..3888
                     /locus_tag="VFV99_15890"
     CDS             3001..3888
                     /locus_tag="VFV99_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5060849.1"
                     /translation="MGSAAGGVMLGCQRTSKTVAIRDVDHVTTALTSRDGAGVALRRS
                     IGSRALPLLDPFLLLDEIHSSDPADYAAGFPQHPHRGFETVSYVLRGGFQHRDSVGNH
                     GLIHDGGAQWMTAGHGIIHEEMPTQTHGPELWGLQLWVNLPAARKMIRPRYQDLAPSA
                     VPEFDIADARVRLVAGTIGSHRGPVDGIDIRPTMLDATLAPGATFSHSLPGADTAFAY
                     VLEGEVTVGQRQVTAGQLAVLGLGDQVVASSAGGGRFLLVSGTPLREPVARRGPFVMT
                     TDDELRQAFADYQNGTLTL"
     gene            complement(3864..5732)
                     /locus_tag="VFV99_15895"
     CDS             complement(3864..5732)
                     /locus_tag="VFV99_15895"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HEX5060850.1"
                     /translation="MCTAADATWFSPRASTVLRALFCTYVALTALHIGYVVHHEPFAF
                     DAWNVAVDTHAQPASVRGFITYWVDQYTSSNPRIGQPLAYLAYKTTGVAELVTPLAYL
                     ALVLGGFVLALRRAPVRHDLRDLATLAIGIGCLWFAAPNFPAYLFCRAYATNYVWVAA
                     LEIWFLVALRAWSVRPSRWRIAAIAVLGVAAGMGNEHVGPTLIAFALAFALYDREKYG
                     AHRAVLAAGVGGVIAGYALLFLAPGQGQRYDGLAAKLSLFDQVVTRGAAGNLDIVRGL
                     LFAASPLLALIIAIAIIGLVQRTVIERHQRGALAVVGLALLAGVTIAITLFVSPLLGP
                     RFYLHAMFVLLAAVMALARAFLTRTTPFVILAVVASGYAAARTVPLYTRLAAASDLRL
                     AELAATEPGDTYTARAWEQVPESWWFLGDDTRDQRKRDLIASYYQLGRVVFRAGERWS
                     PLGVTDVKLQTDYAFDPPPSRSEAQAIDAQFAAAQYLGRDVRALQHAFLDAIAEVQRD
                     ATTALRAVDLIVTFDGPPPRLPRDRVYVARWRDGALESYAAQIVRHGRSPDREVELPP
                     ALADWEPFVVALGDPPRRLRGHTYRPWRSGEYWLLACKREYCFVVAATNHSVSVPF"
     gene            complement(5844..6422)
                     /locus_tag="VFV99_15900"
     CDS             complement(5844..6422)
                     /locus_tag="VFV99_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060851.1"
                     /translation="MVGRLVLALALLGCHGADPAKPAPAKATAPAPTWPVPQGWRSEV
                     IRFPLDFAPSLPHRGYEDLRFPPGMFDPAAPGYWSYAFAWRTDDPAELDATALAAELT
                     TYFRGLIAAVDDKHRITARDEIAVEATADGNRFALTAHVFDAFKTAQPIVLTGWAERR
                     ACATGALWVFVLSREGSAFRATLDDLARQAGC"
     gene            6463..6705
                     /locus_tag="VFV99_15905"
     CDS             6463..6705
                     /locus_tag="VFV99_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060852.1"
                     /translation="MTNALEELSPYYFQAFGVTDTQGWIMWKVVLTILRRFEETGITQ
                     QELPRSIQRPGAAVALAVALGIRQAGLSASAATAQR"
     gene            complement(6689..7093)
                     /locus_tag="VFV99_15910"
     CDS             complement(6689..7093)
                     /locus_tag="VFV99_15910"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEX5060853.1"
                     /translation="MAGDRCACGSGVIEAACCGNFLDGVPAPTALALMRSRYTAFVRG
                     AIDYLIETHDASTRGTVDRKSIAQWSRDTTWQGLEIVATERGGEDDDDGIVEFIARGI
                     TNGKPFAQRERSRFRKLDGLWYYVDGKISAAR"
     gene            7117..7242
                     /locus_tag="VFV99_15915"
     CDS             7117..7242
                     /locus_tag="VFV99_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060854.1"
                     /translation="MLNRADDLANMTVLDEQGTPVALGTFWKDKPAVLVFTRHFG"
     gene            7261..7644
                     /locus_tag="VFV99_15920"
     CDS             7261..7644
                     /locus_tag="VFV99_15920"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX5060855.1"
                     /translation="MVQLHQGIDQLHAAGAELYIIGNGTPNFIAGFREQTKYPDPIYV
                     DPSLAVYKAAELKRGALRTFDPRALGKTITAFRHGSRQGSVQGDAWQQGGVLVVAPGG
                     EVKFHHASDRPGDNASIPEIVAALR"
     gene            complement(7641..8516)
                     /locus_tag="VFV99_15925"
     CDS             complement(7641..8516)
                     /locus_tag="VFV99_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060856.1"
                     /translation="MTRQLLIAFAILASSSLAYADLSKEACVDAHSRGQDARDSGKIT
                     LARKLFLTCAQSGCPAAVQGDCARFADDLSNLQPTVNFVARDGNGNDLPNTSVYVDGM
                     LVATSIDGRPVDVDPGNHTVKFSNAGKDEVVTVVIGSGEKGRVVQARFGSATIVSNPG
                     GGIVDRPAPKKQGPTVVHPKGALAVAIAGGVLALAGGGVVFYGRSQMPSQCDLSTHEC
                     AAPPGDKVFGEASSAASTINLGYATAGVGVAVLAGGLVWYFAGAKTIKESSEQQAVAP
                     WVNANGGGVAVMGRF"
     gene            complement(8526..>9408)
                     /locus_tag="VFV99_15930"
     CDS             complement(8526..>9408)
                     /locus_tag="VFV99_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060857.1"
                     /translation="RMLSAIEPVVQQGLDVDLAKRYASAADMEEALTRAVPPASTSEV
                     AAWVKSLGKDFIESRDKIIAAEEASWRRTHANIATQPGVRRVATLPGEFRRATSAAEL
                     QHEPLATESAPPPRAGGRAIIGVLAALVLLLGVGAVVAMKSGDAAPEPAAKPTPPAEP
                     TPTAAAAPVPEPTPAAVTEPAPTAPEPATESAPVAAMPEPTPPPSETSAEKPVVTREA
                     PPVKRAPVRPAPRPAVVKKEIKKSEEVPPAKPTPNPVTQAVGSDAKPAAADKPKDDCN
                     PPYYFEGSKKVFKPACL"
BASE COUNT         1561 a         3101 c         3206 g         1540 t
ORIGIN      
        1 gacgaagaag tcccagagcg cgtcggtcgc gatgaggtcg ttgctgttgt tgccgaggaa
       61 cggcacggtc tcgccgcctg gccactgatg cccgccgccc tgcatcgtgc acaggaccac
      121 gtccgcgcca tcggtacagc cgccgtgggt cacgcaggtg gcgacaccgt tctggtacgt
      181 cgtcgcggtc gtcgtgcagt ggttctttgc gacccagtag ctcacggtct cgtcgaccca
      241 gtcgtagctg acgagctggt cggcggtgcc gtgcacgaga aacaccggca tcggcttggt
      301 cggcgcgcag gtcggcagct ggagcaggcc agcgaccgca ccgatcgcac ctacacgatc
      361 ggtcagctcg cacgcgacgc gatggacgag ataaccgccg ttcgacaggc cctcgaaaaa
      421 gatgcgcgtc gggtcgacgc acagccgcgc ttcggcttcg tcgagcaccg cagtcatgaa
      481 gccactgtca tcgatgttgt tcgacgccgc gggatcgcag cagccgccac cggcgttcca
      541 gctcgtcggc gagccccacg cttcgggatg gatcacgatg aagcccgccg catccgactt
      601 cgcgatcgcg tgcgagagcc acgcctgctg gctcgcgttg ctcgtgcggc cgtggaggtt
      661 gatcacgagc ggcgtgcgct tgctcggatc gtaactcgcg ggtacgtgca cgcgtgcggt
      721 gcgggtcagc gagccgaccg tgagcgacca gtcactgtcg agcggctgtg cggtcttgcc
      781 gtcgcacgtc gcgctgatct gcgcgtcgct gtcagagccg ccgctgtcgc tgccgagtgc
      841 ggcatcaccg gcgacatcgt catcgtcgcc gtgctgcatc atcgtcgggc ttccgcacgc
      901 ggcgagaccg aggaacgcta acgctcgcgc cagcgagccg ccaggcgagc aagtaccctt
      961 ggggtgcatc acgaccgtgg gtgcgcgacg aagaaatccc agagcgcgtc ggtcgcgatc
     1021 aggttgtcgc tcttgttacc gaggaacggc agcgtctcgc cgccgggcca ctgatgaccg
     1081 ccgtcctgga tcgtgcacag cacgacgtcg gcgcccgcgg tgcagccgcc gtacgtcacg
     1141 cagctcgcat cgccgttcgt gtatgtcgtg gtcatcgtcg tgcacccgtt cttgcccttc
     1201 cagaagttca cgcccgacgc gacgtactgg tacagcgaga gcgagtccga cgtgccattg
     1261 accatcatca ccggcatcgg cttcgtcggc gagcagccct ggaatagcaa gagtccggcg
     1321 accggaccga tggccgcgat gcgatcggcc atctcgcagc cgagccggtg cgattcgtac
     1381 gcgccgttcg acatgcccat catgtagacg cgcgaggtgt cgatgcacag cttcgcttcg
     1441 gcttcgtcga gcagcttctt gaagaagccg gtgtcgtcga ggttgctcga cgaggcgggc
     1501 tcgcagcacg tgcccgagtt ccagctcgtc ggcgtcgttg ccgattctgg atagatcgcg
     1561 atgaagccag cagcgtccga cttcgcgatc gaatgcgtga gcgacatctc cccggatgca
     1621 ttcgccgtgc ggccgtgaac gtcaaccacg agcggcgtgc gcttgctcgg atcgtagctc
     1681 gcgggcacgt gcactttcgc gacgcgcatc gagctgccga ccatgatgtt ccacgtcgca
     1741 tcgagcggct gcgcggtctt gccgtcgcac gtcgcgctct gcggtggcgc atcgctcgag
     1801 cccgagccca tatcggaacc gccgccgccc gctgcatcga gttgatcaat ggattgcgtc
     1861 ggcgagctac acgcgaccaa ccacacaaga agccacgctc tcatcgcgca cgaggaagcc
     1921 agtttccgac gacgcgagca acgacaaatc gtgcatcgca cgtcgaatgc gccagtcgcg
     1981 cagcgaggtg cgctaaccga tcaagttgag cagacgctct ggatcgtcgc tctcgatcca
     2041 gtcgacaccg agcgcgacga gccgctcgac ctcttgcgcg gtcatcgcac tcggcgagat
     2101 cggcttgccg gtcgtcgaac cgagcgggaa caacgtgtgc gtaccgatcg cgatgccctg
     2161 atcgtgcagg cgcttcacgg ttcgactgcc cacaatcgtg tgctccgcgc cgaccacacg
     2221 tgtgccgagc agcttgccca cgaggttcgt ctcgagaatg cggttgagca gccgacgcgc
     2281 gttcgtgcgg atgtgaccgt cggcgggcag cgacagcttg tttcgcagat acggcaggcg
     2341 ctcgaggaac ggcgccgcga agtcgagcat cgtgtcgtcg aagcagaaac cggtcgcgag
     2401 cttcggatac gccgacagcg cagcgcgcag cttgcgcgga tcgaagctgg tgaggatgac
     2461 gtgatcctcg gcgttcgcct tgcgcacttc ctcggcaacg acgcgcgcga tctcggtctg
     2521 ccagaagccg aacgcgttga gcttgagctc gatgttgatc gcgaccttgc ccgcgacttc
     2581 ggcgagtact tcggcgagca gcggaatcgg ctcttcgcgc tcgtaccgcg cgaccgtgcg
     2641 ttgaccatgg acatcgacac ccatcggcag ctcgcgacga acgcgcagct tcgacaattg
     2701 gtcccacgtc agatcccata cgttgcgcgg gttgccggtg agccggcgca gattcgcgtc
     2761 gtgcaaaaca actgcacggc gatcagcggt gagctgcacg tcgagctcga tcgcggggac
     2821 accgagctgc acggcgcgct tgaacccggc gagcgagttc tcctggtgcc gcgcagggac
     2881 accacgatga cccacgacga tcggcttctt cttgccgagc atgaatgggt tagacagcgc
     2941 cgcgtgcatg gtgtttcctt ccactgtagc gtgttgttga tttcgcgacg acgattcctt
     3001 atgggctccg ccgccggcgg ggtaatgctg ggatgccagc gcacgtccaa gacggtagcg
     3061 atccgtgatg tcgaccacgt cacgaccgcg ttaactagcc gtgacggcgc gggcgtcgcg
     3121 ctgcgtcgat cgatcggtag tcgtgcgctg ccgctcctcg atccgttcct gctgctcgat
     3181 gaaatccata gctcggaccc ggcggattac gcggccggct tcccccagca cccacatcgc
     3241 ggattcgaaa ccgtgtctta cgtgctccgc ggcggcttcc agcaccgcga tagcgtcgga
     3301 aatcacggcc tgatccacga cggcggcgcc cagtggatga ccgcaggcca cgggatcatt
     3361 catgaagaga tgccaaccca gacccacggt cccgagctat ggggcctcca gctatgggtc
     3421 aacctgccgg cagcccgcaa gatgatccgg cctcggtacc aggacttggc gccgtccgcg
     3481 gtccccgaat tcgacatcgc cgacgcgagg gttcgcctgg ttgcgggcac gatcggctcc
     3541 caccgggggc ctgtcgatgg gatcgacatc cggccgacca tgctcgatgc gacgttggcg
     3601 cccggcgcga ccttcagcca ctcactaccc ggcgcagaca ctgcgttcgc gtacgtattg
     3661 gagggcgagg tcacggtggg ccaacgccag gtcacagctg gccagctcgc ggtgctgggc
     3721 ctgggcgacc aggtcgtggc gagctcggca ggcggcggcc ggttcctgct cgtgtcaggc
     3781 acgccgctac gcgagccggt cgcgcggcgc ggtccgttcg tgatgaccac ggacgacgag
     3841 ctgcgccaag cgtttgccga ctatcagaac ggcacgctga cgctgtgatt cgtcgccgcg
     3901 acgacgaagc aatactcgcg cttgcaggcg agtagccagt actcgccgct tcgccacggc
     3961 cggtaggtgt ggccgcgcaa ccgccgcggc ggatcgccga gcgcgacgac gaacggctcc
     4021 cagtccgcca gcgcgggcgg cagctccacc tcgcgatcgg gtgaacggcc gtggcggacg
     4081 atctgcgcgg cgtagctctc gagcgcccca tcccgccagc gcgcgacgta gacgcgatcg
     4141 cgtggcaggc gtggcggtgg gccgtcgaac gtcacgatca gatcgaccgc gcggagtgcc
     4201 gtcgtcgcgt cgcgctggac ctcggcgatc gcgtcgagga atgcatgctg cagcgcgcgg
     4261 acgtcgcggc cgaggtattg cgcggcagcg aactgggcgt cgatcgcttg cgcttcgctc
     4321 ctcgagggcg gcggatcgaa tgcgtagtcg gtctgtagct tgacgtccgt gaccccgagt
     4381 ggcgaccagc gctcgccggc gcggaacacg acgcggccga gctggtagta gctcgcgatc
     4441 aggtcgcgct tgcgttgatc gcgcgtgtcg tcgccgagga accaccacga ctcgggcacc
     4501 tgttcccagg cgcgcgccgt gtacgtgtcg ccgggttcgg tcgcggcaag ctcggcgagt
     4561 cgcaggtcag aggcagcggc gaggcgcgtg tagagcggga cggtgcgcgc ggccgcatag
     4621 ccgctcgcga cgacggcgag gatcacgaac ggtgtcgtgc gcgtgaggaa cgcgcgcgcg
     4681 agcgccatca cggcagcgag cagcacgaac atcgcgtgca gatagaatcg tggaccgagc
     4741 agtggcgaca cgaacagcgt gatcgcgatc gtgaccccgg cgagcagcgc gaggccgacg
     4801 acggccagcg caccgcgctg gtggcgctcg atcacggtgc gctggacgag gccgatgatc
     4861 gcgatcgcga tgatcagcgc gagcagcggc gaggcagcga acaagaggcc gcgtacgata
     4921 tcgaggttgc cggcggcgcc gcgggtgaca acttgatcga acagcgacag cttcgcggcg
     4981 agaccgtcgt agcgctggcc ttgacccggc gcgaggaaca acagcgcgta gccggcgatc
     5041 acgccgccga cgcctgccgc gagcaccgcg cgatgcgcgc cgtacttctc gcgatcgtag
     5101 agtgcgaacg cgagtgcgaa tgcgatcagc gtcgggccga cgtgctcgtt gcccatgcca
     5161 gcggcgacgc cgagcacggc gatcgcagcg atgcgccacc ggctcggccg cacggaccac
     5221 gcgcgaagag cgacgagaaa ccagatctcg agcgccgcga cccacacgta gttcgtcgcg
     5281 taggcgcggc agaacagata cgcgggaaag ttcggcgccg cgaaccacaa gcagccgatg
     5341 ccgatcgcga gcgtcgcgag gtcgcgcaga tcatggcgca cgggtgcgcg gcgcagcgcg
     5401 agcacgaaac cgccgagcac gagcgcgaga tacgcgagcg gcgtgaccag ctcggcaaca
     5461 cccgtcgtct tgtatgcgag gtacgcgagc ggctggccga tgcgcgggtt gctgctcgta
     5521 tactggtcga cccagtacgt gatgaagccg cgcacgctcg cgggctgcgc gtgggtatcg
     5581 actgcgacgt tccaggcgtc gaatgcgaac ggttcgtgat ggacgacata cccaatgtgc
     5641 agagcggtca gcgcgacgta cgtgcaaaac aatgcgcgca gcacggtgct cgctcgcggc
     5701 gagaaccagg tggcgtcggc agcggtgcac atcgagtggg cccgttcagc aacggctgtg
     5761 cccgcgcaag gggtgagaag tgcgtggcga cgacgccggt aaatcgtggt tcaccccaaa
     5821 acccggctcg aaatgtcgtg gtctcagcag ccggcctggc gcgccaggtc atcgagcgtg
     5881 gcgcgaaatg cgctgccctc gcgggaaagc acgaacaccc agagcgcgcc cgttgcgcag
     5941 gcgcggcgtt cggcccaccc ggtgaggacg atcggttgcg cggtcttgaa cgcgtcgaag
     6001 acatgcgcgg tcagcgcgaa ccggttgccg tcggcggtcg cctcgacggc gatttcgtcg
     6061 cgcgcggtga tgcggtgctt gtcgtcgacg gcggcgatta gcccgcggaa gtaggtcgtg
     6121 agctctgcgg ccagcgccgt cgcatcgagc tcggccggat cgtcggtgcg ccaggcgaat
     6181 gcgtaggacc agtagccggg cgcagccgga tcgaacatgc cgggcgggaa ccgcaggtcc
     6241 tcgtagccgc gatgtggcag cgacggtgcg aagtcgagcg ggaagcgaat gacctcgctg
     6301 cgccagcctt gcgggaccgg ccacgtgggc gcaggagccg tggccttcgc gggcgctggc
     6361 ttcgcgggat ctgcaccgtg acagccgagc agggcgagcg cgagcacgag acggccaacc
     6421 atcgcggaca tcatgtcaga cctagcggat acggtgcgcg ccgtgacgaa cgcactcgag
     6481 gagctgtctc cttactactt ccaggccttc ggtgtgaccg atacgcaagg ctggatcatg
     6541 tggaaggtcg tgctgacgat cctgcggcgc ttcgaagaga ccggcatcac ccagcaagag
     6601 ttgccgcgct cgatccagcg tcccggtgca gcggtcgcgc tcgcggtcgc acttggcatt
     6661 cgccaggctg gactcagcgc ctcggctgct accgcgcagc gctgatcttc ccatcgacgt
     6721 agtaccagag accatccagt ttccgaaatc gcgagcgctc gcgctgcgcg aacggtttgc
     6781 cgttcgtgat gccacgggca atgaactcga cgatgccgtc gtcgtcatcc tcgccgccgc
     6841 gctcggtcgc gacgatctcg agcccctgcc atgtcgtgtc gcgcgaccac tgcgcgatcg
     6901 atttgcgatc gacggtgccg cgcgtcgacg catcgtgcgt ctcgatcaag taatcgatcg
     6961 ccccacgcac gaatgccgtg tagcgcgagc gcatgagcgc gagtgcggtc ggtgccggca
     7021 caccgtcgag gaagttgccg cagcatgcgg cctcgatgac acctgaaccg catgcacatc
     7081 gatcgccagc catgcgcgta gggtacgata ggcacaatgc tgaatcgcgc cgacgacctc
     7141 gcaaacatga ccgtgctcga cgaacaaggc acgccggtcg cgctcggcac gttctggaag
     7201 gacaagccgg ccgtcctcgt gttcacgcgc cactttggtt gaattcactg ccgcgagcac
     7261 gtggtgcagt tgcaccaagg aatcgaccag ctccatgccg ccggcgcgga gctctacatt
     7321 atcggcaacg gcacgccgaa cttcatcgcg ggctttcgcg agcagacaaa gtatcccgac
     7381 ccgatctacg tcgatccctc gcttgccgtg tacaaggccg ccgagctcaa gcgcggcgcg
     7441 ctgcgcacgt tcgacccgcg cgcgctcggc aagaccatca cggcgttcag acacggctcg
     7501 cgccagggca gcgtgcaggg cgatgcctgg cagcaaggcg gcgtgctcgt cgtcgcgccc
     7561 ggcggcgagg tgaagttcca ccacgcctcg gatcgcccgg gcgacaacgc gagcattccc
     7621 gagatcgtcg cggcgctgcg ctagaagcga cccatcaccg caacgccgcc gccgttggcg
     7681 ttgacccatg gcgcgaccgc ttgctgctcc gagctctcct tgatggtctt cgcgccggcg
     7741 aagtaccaga cgaggccgcc ggcgagcacg gcgacaccga cacccgccgt cgcatagccg
     7801 aggtttatcg tcgacgccgc gctgctcgct tcgccgaaca ccttgtcgcc cggaggcgcc
     7861 gcgcactcgt gcgtcgatag atcgcactgg ctcggcatct gcgacctacc gtagaacact
     7921 acgccgccgc cggcgagcgc gagcacgcca cccgcgatcg cgacggccag tgcgcccttc
     7981 gggtggacga cggtcgggcc ctgcttcttc ggcgccggtc tatcgacgat gccgccgcct
     8041 gggttcgaga cgatggtcgc cgagccaaag cgcgcctgga cgacgcgacc cttctcgccg
     8101 ctgccgatca cgacggtgac gacttcgtct ttgcccgcgt tcgagaactt gacggtgtgg
     8161 ttacccggat cgacgtcgac ggggcggccg tcgatcgacg tcgcgacgag catgccgtcg
     8221 acgtacacgc tcgtgttcgg caggtcattg ccgttgccgt cgcgcgcgac gaagttcacc
     8281 gtcggttgca ggttcgagag atcgtcggcg aacctcgcgc agtcaccttg gacggcagca
     8341 gggcagcctg attgtgcgca ggtcaagaac agcttgcgcg caagcgtgat cttgcccgag
     8401 tcacgcgcgt cttggccgcg actatgcgcg tcgacgcacg cttctttgga aagatcggca
     8461 tacgcgagcg agctcgacgc gagaatcgca aacgcaatga gtagctggcg ggtcattcga
     8521 gtctcctaga ggcaggcggg tttgaagacc ttcttcgagc cttcgaagta atagggcggg
     8581 ttgcagtcgt ctttgggttt gtcggcggct gccggcttgg catccgagcc gacggcttgg
     8641 gtgactggat tgggagtggg cttggcgggc ggtacttctt ccgacttctt gatctccttc
     8701 ttgacgaccg cagggcgcgg cgcaggacgc acgggtgcac gtttcacggg tggggcttca
     8761 cgcgtgacga ccggcttctc ggccgacgtc tcgctcggag gtggtgtggg ctcgggcatg
     8821 gcagcgaccg gcgcggactc cgtcgccggt tcaggcgcgg taggcgctgg ctccgtcacg
     8881 gcagctggcg tcggttcggg cacgggcgcg gccgcagcgg tcggtgtggg ctcggccggc
     8941 ggcgtgggtt tcgcggcggg ctccggcgca gcgtcgcctg atttcatcgc gacgacggcg
     9001 ccgacgccga gcaacagcac gagcgcggcg agcacgccga tgatggcgcg accaccagct
     9061 cgcggtggcg gcgccgattc cgtggccagt ggctcgtgtt gcagctcggc ggcgctcgtc
     9121 gcgcggcgaa actcgcccgg caacgtcgcc acccggcgaa cgccgggctg cgtcgcgatg
     9181 tttgcgtgcg tgcggcgcca gctcgcctct tcggcggcga tgatcttgtc gcgactctcg
     9241 atgaagtcct tgccgaggga cttcacccac gcggcaacct cggacgtcga tgccggcggc
     9301 accgccctgg tcagcgcctc ctccatgtcg gccgcgctcg cgtagcgctt cgcgagatcg
     9361 acgtcgaggc cctgctggac gaccggttcg atcgccgaca gcatcctc
//
LOCUS       DASUFX010000399        11434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_204132,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000399 DASUFX010000000
VERSION     DASUFX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204132"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFV99_15935"
     CDS             complement(<1..568)
                     /locus_tag="VFV99_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5060858.1"
                     /translation="MSRTRNSTGTAITGAGVWHPENVLTNEEICVAFNEYVRRTNAAG
                     AQPPLKESSPEFIEKASGIKRRYIVDKTGVLDPERMCPNVPDRRDDQLSVQAEFGVNA
                     ITRALAAAGRVGEEVDLVVCGTSMLQRAYPAMAIEIQNAIGARGLGFDLTLGCASATA
                     AIQVATTAIQAGLATCAVVVSPELMSGHN"
     gene            complement(686..1609)
                     /locus_tag="VFV99_15940"
     CDS             complement(686..1609)
                     /locus_tag="VFV99_15940"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5060859.1"
                     /translation="MMNELSAVSVTIPLPTDERDREAALRARFAAYWSTAKGTPRANF
                     DSFISATPIADGVSFERVDNTVVHGWWARCAEPAPDGVILHLHGGGYVQGSADAFRPL
                     ASQIVGRTQLTTFVLDYPLAPEATLPTAVDAVMAAYDWLLTTANRIAIVGDSAGGGLA
                     LALMSRLAQRGPQAIAGVLFSPWVDLAFAGASMTDATIADPLITYEHLQAYAQKYLGR
                     ADPRDPLASPLYGDLSHLPPLLIQVGTDEHLLDDARRLADRAARAGSPVRLEIWEGMH
                     HVFQLNVADLHTSRRALNHATAFLASHVCGA"
     gene            1698..3887
                     /locus_tag="VFV99_15945"
     CDS             1698..3887
                     /locus_tag="VFV99_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5060860.1"
                     /translation="MRWVAVLLLVGCVQTNARFCADGRICPEGRLCDDVHDLCVSQDQ
                     RDQCHDKPDHTHCVSDVTTGQCDQGVCIPGCGDGEQQAGETCDDGGFANHDGCSSVCE
                     LEVGNWREVPKYWVARAGHVAAFDTARQKLVVFGGVTQASATANAGSSNDTWERSADG
                     TWHQIEVAGPPPRQNAAMAYDAARGVMVLFAGVTTQGTVLTDTWEFDGTTWTQKAPTV
                     SPPPRRLHAMAYDSTRQRVVVFGGASETLQPFGDLWEFDGTTWSPIATTGTPPAPRYS
                     AAMAFDDNRDRLVVFAGSTFDGQTWELAPNGAWSLIPVTTGPKNRLAGAMTYDPVSQR
                     VLLFGGDDLGGVLNDFWAWDGATWTVLTSPRTPAPRRGHTMTSVGTTTVLVAGTAEIS
                     DEPLDDVWTYEPVQGWKLVDLNFIPSPRAATPLAYDPVRDELVLYGGLLLHNDTWTFD
                     GTRWFAEMTPQTAGLRTYHSTAWDAARQRVVIYGGLAGGTTIPYDDAWAWDGTRWTRI
                     QAVTRPPGRASAAFAGNAAGVLVMFGGGTALDPSGALQDTWELEGSTWRQIETATAPG
                     PVFGPAMTYDPEHDRMVLYDRDGATWTYRNGTWTLLVPASEAPGPRNFATLVYHEARG
                     RVVLYGGVAGSIVLTDLWELDGTTWRQVNAAGEPPAAQFSSVAYYPTQRSIVLFGGQR
                     SNVINETWSFRYDSTLPDEICTNGSDDDEDGLADCDDPDCIYHGCPN"
     gene            3902..4744
                     /locus_tag="VFV99_15950"
     CDS             3902..4744
                     /locus_tag="VFV99_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060861.1"
                     /translation="MVTNGRRWLFVALAGCGGGGSGNVDAPLTIVDAPPDAKVWLDAP
                     PPEFDFSCMTNPPPTTANANITGSGTIRRIEFNGSIMFNPLDGASVRVCKAPGAPDCN
                     TTGNQLGSTVTTANGGLWSITFPSGGQPADVYVEMTEATSRTTFAFPDQPLTMDAMAD
                     LLTFTPQVIAALSLVPNGCTQSAGNALIGLAVIDCANQPIADTANLTLSIKQGGNEVT
                     GTTTMDLGAVNGAAAGLFLICNVPPNNVTTVGASWKGMALRPHDVKTVAGTTSQTIVR
                     PGYK"
     gene            4749..5720
                     /locus_tag="VFV99_15955"
     CDS             4749..5720
                     /locus_tag="VFV99_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060862.1"
                     /translation="MKRLLITAMLCSASIASAQPWAEGVSDAHKAEAQARLEEGNALF
                     LKQSYREALEKYQDALRAWDHPSIRFNIVRCLIQLDRPVDAYDNLKAALKYGAEPLEE
                     NVYREALAYEKLLANEVAEVEIKCAQPGVAITLDGQDLPTCPGQATRRVKPGKHEVVA
                     NKAGFLTVQRELKVVGGSRESVDIALVPLAEATKVVHRWPTWIPWVVFGSGLGVAGFG
                     AAMEYNAQRTMDEHDREVARDCAMGVCYADPNADGLTDAQRAKARDLQTQRANAELQD
                     KIAIGIISVGTVAAIAGGVMLVMNRGETVYSLEPQPGGSMVTASGRF"
     gene            complement(5721..8543)
                     /locus_tag="VFV99_15960"
     CDS             complement(5721..8543)
                     /locus_tag="VFV99_15960"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060863.1"
                     /translation="MGNEGNPALHATELPPVAQRPKAVPEAFHATQSEPGADAGVAIA
                     ASPGQRVHHYELIRELGRGGMGVVWAARDTKLGRRVAIKFLRQASREDAERFLTEARA
                     TAQCNHDNIVIIHEVDELDDTPYMVLEFLEGQTLRTMMGPIGGASRLPATRVVELVLP
                     IARALARAHEGGIIHRDLKPENVIVTTAGQVKVVDFGIAKVKDSRHDLMAGTLPYMSP
                     EQLGMGEIDQRTDLWALGVMMWEMLAGRHPIEPFTGEALFANARNDTPMPTLRSAVED
                     APDALAGLVDECLRKTHADRIANAGELVRRLEALAPGAGRATRTLAETESPYPGLTAF
                     QEGDAHRFFGRARDIARITARIRDVPITALVGPSGVGKSSIVRAGVGPALKASGEQWD
                     VVTLRPGRQPLAALATIVQRLTTKSGSHLKEEVGEHEQLVTRLRTEPGYVGTLLRARA
                     RQSGGHILLFVDQFEELYTLVTDAEERRTFTAALAGVADDTAAPLRVVLAMRSDLLDR
                     VGEDARFMEELSRGLVFLRPPDREGLREALVAPVEMVGFHFESNAIVGDMIDALADTP
                     GGLPLLQFAAAKLWDARDRDKRLLTVRSYAAIGGISGALATHADDVVGQMNAAAQKLT
                     QQMFRRLVTPDRTRAIVELADLQQLAGDPAEVARTIDTLVAGRLLVVQTRADGGTGSV
                     EIVHESLIDRWPTLRRWLDEDQEDAAFLAQLATAAKQWDAKGRPHGLLWRGDAMEEAR
                     RWHALRPRELPAREQAFLDDVFALARRGARAKRLALIVAFTVLGCVAIGAIVAAAWIR
                     NAEVVAQQEKHKAQDALTAMEAKEAARKAAEAKQKAAEAQQQAALAEKHEVEEAKQKV
                     EAAKEKAETAVAETAEELRKKNIALEQKIIEASTAKERALREKETAVAISAQLAKAKD
                     ELKLANDRLKEENERLKKEVLATKLK"
     gene            8620..10395
                     /locus_tag="VFV99_15965"
     CDS             8620..10395
                     /locus_tag="VFV99_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060864.1"
                     /translation="MRYLYGDSTSSPLQSNYLAFFRDAMEFWVHLLLAQQRINKLRDE
                     RSATDQYAAAERTRIGGLKALVTDAAEEANHDGAESMSLRAVKSVITASDQAIAVILD
                     ELQRKLDADRAALAAKDRHERDECLDAFAKYVALHEAHEGTWRLKAYLDDDGSYQGET
                     SGAAPYGAEWRCAIELQSDHPMAKGIKVGDLVTQIELPLPEGSGWLKKGKLKPQRIDQ
                     YSIDSFETDGTQFSYALRATPRAPTGLDVTIRGDKIEVTLADAKDATSVEVNLDNRER
                     LLTLRDRILDALTSHAGVRRRVVTATIDDKPLADGDDLGDVVKRMIDAATPIVKQIRK
                     HSLSPHELVIRRLLGNDRREEIFVSTASLLEKLGRLPRNLRSLFEPLGLEWQRAHTPV
                     PGEIFVEPPRPTAPEPEPAKVEVANAEPERQQSETTESAPSEPSRASVPEPVDDAAGS
                     AAGSIEAALLRTSSPSIEVAPVAAASVPSPPVTSTISIDAHSKEALAATVKRIVGMAR
                     EGRTPEAYAAYAALFEDNGFAQQRPQDQRQVLKLVLMSKTPPPRSESVTHAYRSALAR
                     LQSLSADSRDPIDDEMIGVCLALLG"
     gene            10437..11024
                     /locus_tag="VFV99_15970"
     CDS             10437..11024
                     /locus_tag="VFV99_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX5060865.1"
                     /translation="MKKSAPAALRNREPIREVLAQQLPETGTVLTIAEGSGEHVVHFA
                     RSFPALTWQPSDVDAEALASIAEWREETALPNLASPVQLDVTAAQWPIAHADVITCIN
                     MIHIAPWEATLGLFAGAARTLPSGALLYLYGPYRFDGAFTAPSNEAFDASLRARDSRW
                     GVRDVSDLQTAATGFVLRAVVEMPANNHSLVFRRN"
     gene            11150..>11434
                     /locus_tag="VFV99_15975"
     CDS             11150..>11434
                     /locus_tag="VFV99_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060866.1"
                     /translation="MSARADISPRGVLSAGIDIGHHDHPDDNGTSIGVNLDGELHVGM
                     FLVGASIGYDDYAPLNGVTVHAGTYAARVGVDVPFAKTAPNRKGRTIQFDA"
BASE COUNT         1962 a         3729 c         3787 g         1956 t
ORIGIN      
        1 tgttgtggcc gctcatcagc tcgggcgaca ccacgaccgc acacgtcgcg agtcccgcct
       61 ggatcgcggt tgtcgcgacc tggatcgccg ctgtcgctga cgcgcatccg agtgtcagat
      121 cgaagccgag tccgcgcgca ccgatcgcgt tctggatctc gatcgccatc gccggatacg
      181 cgcgctgcag catcgacgtc ccgcagacga cgagatcgac ctcttcgccg acacgcccgg
      241 cagcagcaag cgcgcgcgtg atcgcgttca cgccgaactc ggcttgcacc gatagttgat
      301 cgtcgcgtcg atcgggcacg ttcgggcaca tgcgctcggg atcgagaacg ccggtcttgt
      361 cgacgatgta gcgacgcttg atgccggaag ctttttcgat gaactccggc gacgattcct
      421 tcagtggcgg ctgggcgccc gccgcattgg tgcgtcgcac gtactcgttg aacgcgacgc
      481 aaatctcttc gttcgtgagg acgttttcgg gatgccagac accggcgccg gtaatggccg
      541 ttccggtcga attgcgagtg cgtgacatgg ccgtggcatc gcatagccga acccctgtcc
      601 gcaccagcaa tccgtcatgc ttgggtcagg ttcgaccacg taagtcttga aacgtgcacg
      661 tgaagcgcgc atgaagcggg gtctattagg ctccgcacac atgtgatgcc aggaacgcgg
      721 tggcgtggtt cagcgcgcgg cggctcgtgt gaaggtcggc gacgttgagc tgaaacacgt
      781 gatgcatccc ttcccagatc tcgagccgca ccggagagcc cgcgcgtgct gctcggtcgg
      841 caagccgccg ggcatcgtcg agcaggtgtt cgtcggtgcc cacctgaatc agtagcggtg
      901 gcagatgtga cagatcaccg tagagcggtg atgcgagcgg atcgcgcgga tcagcacggc
      961 cgaggtactt ctgcgcgtag gcttgcaggt gctcgtacgt gatcaacgga tcggcgatcg
     1021 tcgcatccgt catcgacgcg ccggcaaacg cgagatccac ccacggcgag aacagcacgc
     1081 cggcgatcgc ttgcggtccg cgctgcgcga gtcgcgacat caacgcgagc gccaagccac
     1141 cacctgcaga atcgccgacg atagcgatcc gattcgccgt cgtaagcagc cagtcatatg
     1201 ctgccatgac cgcgtccact gcagtcggca gcgtcgcctc gggtgcgagc ggatagtcga
     1261 gcacgaaggt ggtgagctgg gtgcggccca cgatctgact cgcgagcggc cgaaatgcgt
     1321 ctgctgatcc ttggacgtag ccgccgccgt gcagatgcag gatcacgcca tcgggcgctg
     1381 gctccgcgca gcgcgcccac cagccgtgga ccacggtgtt gtcgacgcgc tcgaaggaaa
     1441 cgccgtcagc aatcggcgtt gcggagatga acgagtcgaa gtttgcccgc ggcgttccct
     1501 tcgcggtgct ccagtacgct gcgaatcggg cgcggagcgc agcctctcgg tcacgctcat
     1561 ccgtcggcag agggatcgtg acgctgaccg ccgagagctc gttcatcata gttcctcctc
     1621 gaatcacggc ggtgcaccgc gcgtaccacg cacgcttccc gaaacagcag cgctggtcgc
     1681 ggtatcatcc gtagctggtg cggtgggtcg cggtcctctt gctggtcggg tgcgtgcaga
     1741 cgaacgcgcg gttctgcgcg gacggtcgca tttgtcccga agggcgtttg tgcgacgacg
     1801 ttcacgatct gtgcgtgtcg caagaccagc gcgatcagtg tcacgacaag cctgaccaca
     1861 cgcactgcgt cagcgatgtc acgaccgggc agtgcgatca gggcgtgtgc atcccgggct
     1921 gtggcgatgg cgagcagcaa gccggcgaga cgtgcgacga cggcgggttc gcgaatcacg
     1981 acggctgctc gtcggtgtgc gagctcgagg tcggcaactg gcgcgaagtt ccgaagtatt
     2041 gggttgcgcg cgcaggccac gtggccgcct tcgataccgc gcgtcagaag ctcgtcgtgt
     2101 tcggtggcgt gacgcaagcg agcgccaccg cgaatgccgg cagctcgaac gatacgtggg
     2161 agcgctccgc cgatggaacg tggcatcaga tcgaggttgc cggacctccg ccgcggcaga
     2221 acgcggcgat ggcctacgat gccgcgcgcg gcgtgatggt gctgttcgct ggggtgacga
     2281 cgcaaggcac cgtgctgacc gatacctggg aattcgacgg cacgacgtgg acgcagaaag
     2341 cacccacggt atcgccaccg ccgcgccggc tgcatgcgat ggcttacgac tcgacccgtc
     2401 agcgcgtcgt cgttttcggc ggcgcatcgg agacgctgca gccgtttgga gacctgtggg
     2461 agttcgacgg tacgacgtgg tcgccgatcg cgacgaccgg gacgccacca gcgccgcgct
     2521 actcggcggc gatggcattc gacgacaatc gcgatcgcct ggtcgtgttc gccggcagca
     2581 ctttcgacgg tcaaacgtgg gagctcgcgc ccaatggtgc gtggtcgctc atcccggtca
     2641 cgaccgggcc gaagaaccgg ctcgccgggg cgatgacata tgaccctgtg agtcagcgcg
     2701 tgctgttatt tggcggcgac gatctcggcg gggtactcaa cgatttctgg gcatgggacg
     2761 gcgcgacgtg gacggttctc acgtcaccgc gcacgccagc accgcgtcgc ggtcacacga
     2821 tgacctccgt cgggacgacg acggtgctcg tggctggcac cgcggagatc agtgacgagc
     2881 cactcgacga cgtgtggacg tacgagcccg tgcagggttg gaagctcgtc gatctgaatt
     2941 tcatcccgtc gccgcgcgcc gcgacaccgc tcgcctacga tccggtacgt gacgagctcg
     3001 tgctctacgg cgggttgctc ctgcacaacg atacgtggac attcgacggc acgcgctggt
     3061 tcgcggagat gacgccacaa acagccggat tgcgcaccta ccactcgacg gcgtgggacg
     3121 cggcacgcca gcgggtcgtg atctacggtg ggctcgccgg tggcacgacg attccctacg
     3181 acgatgcgtg ggcgtgggac ggcacacgct ggacgcgaat ccaggcggtg acgcgtccgc
     3241 cggggcgagc gtccgccgcg tttgccggca atgcggccgg cgtactcgtc atgttcgggg
     3301 gcggcaccgc actcgacccg agcggcgcac tccaagacac gtgggagctc gagggctcga
     3361 cgtggcgaca aatcgaaacc gcaactgcgc ctgggccggt gttcgggccc gcgatgacgt
     3421 acgaccccga gcacgatcgc atggtgctat atgaccgcga cggtgcgacg tggacgtacc
     3481 gcaacggcac atggacgttg ctggttcccg cgagcgaagc gcccggtccg cgtaacttcg
     3541 cgacactcgt ctatcacgag gcgcgcggtc gcgtcgtcct ctacggaggt gtcgcaggct
     3601 cgatcgtgct cacggatctg tgggagctcg acggaacgac ctggcggcaa gtcaatgccg
     3661 ctggcgagcc accagcggcc cagttctcgt cggttgccta ctatccgaca cagcgttcga
     3721 tcgtgctgtt tggcggacag cgcagcaatg tgatcaacga gacatggtca tttcgttacg
     3781 actccacact tccggacgag atttgtacga acgggagcga tgacgacgag gacggcctgg
     3841 cggattgcga cgatcccgac tgcatctatc acgggtgtcc caactgagag atgatcgcat
     3901 catggtgacc aatgggcgtc gatggctgtt cgttgcactc gccggctgcg gtggtggtgg
     3961 cagtggcaac gtcgatgcac cgctgacgat cgtcgatgcg ccgcccgacg cgaaggtgtg
     4021 gctcgacgcg ccgccgccgg agttcgactt ctcgtgcatg accaatccgc caccgacgac
     4081 ggcgaacgcg aacatcacgg gttcgggcac tattcgtcgc atcgagttca acggctcgat
     4141 catgttcaat ccgctcgacg gcgcgagcgt acgcgtgtgc aaggctcccg gcgcgccgga
     4201 ctgcaacacg acaggcaatc agctcggcag cactgtgaca actgcaaacg gcggactgtg
     4261 gtcgatcacg ttcccgagcg gcgggcagcc tgccgacgtg tacgtcgaga tgaccgaggc
     4321 cacctcgcgt acgacctttg cgttccctga tcaaccgctc acgatggacg cgatggccga
     4381 tctgctcacg ttcacaccgc aggtgatcgc tgcgctgtcg ctcgtgccaa acggctgcac
     4441 ccagagcgcc ggcaacgccc tgatcggact tgccgtgatc gactgcgcga atcagccgat
     4501 tgcggacacg gccaacctca cgctcagcat caagcagggc ggcaacgagg tgacgggcac
     4561 gaccacgatg gatctcggtg ccgtcaacgg agcggcagcg ggtctgttct tgatctgcaa
     4621 cgtgccaccg aacaacgtaa cgaccgtcgg ggcctcgtgg aagggcatgg cgctgcgacc
     4681 acacgacgtc aagaccgtcg cgggcaccac ttcgcagacg atcgtgcgac cgggctacaa
     4741 gtgacgcgat gaagcggctg ctcatcaccg cgatgctgtg cagcgcttcg atcgcgagcg
     4801 cgcagccgtg ggcggaaggc gtcagcgacg cgcacaaggc cgaggcacaa gcgcggctcg
     4861 aagaaggcaa tgcgctgttc ctcaagcaga gctacaggga agcgctcgag aagtaccaag
     4921 atgcgctgcg cgcgtgggac cacccgtcga tccgcttcaa catcgtgcgc tgcttgatcc
     4981 agctcgatcg ccccgtggac gcctacgaca acttgaaggc agcgctgaag tacggcgcag
     5041 agccgctcga agagaacgtg tatcgcgagg cgctcgcgta cgaaaagctt ctcgcgaacg
     5101 aggtcgccga agtcgagatc aagtgcgcgc agcccggtgt cgccatcacg ctcgatggcc
     5161 aggatctgcc gacgtgcccg ggccaggcca cgcgccgggt caaaccgggc aagcacgagg
     5221 tcgtcgcgaa caaagccggc tttctcaccg tgcagcgcga gctcaaagtc gtcggcggca
     5281 gccgcgagtc cgtcgacatc gcgctcgtcc cgctcgcaga ggcgacgaag gtcgtgcacc
     5341 gctggccgac gtggatcccg tgggtcgtat tcggcagcgg gcttggcgtg gcgggctttg
     5401 gcgcggcgat ggagtacaac gcgcagcgca cgatggacga gcacgatcgc gaggtcgcac
     5461 gggactgtgc gatgggcgtc tgttacgccg atccgaatgc cgacgggctc accgacgccc
     5521 agcgtgcgaa ggcgcgcgac ctccagacgc agcgagcaaa cgcggagctg caggacaaga
     5581 tcgcgatcgg tattatctcg gttggcaccg tcgcggcgat cgccggcggc gtgatgctcg
     5641 tgatgaatcg cggcgagacg gtgtactcgc tcgaaccgca gcccggcggc agcatggtca
     5701 cggcgagcgg ccgcttctga tcacttcagc ttcgtcgcga gcacttcctt cttcaggcgc
     5761 tcgttctctt ccttgagccg atcgtttgcg agcttgagct cgtccttggc cttcgcgagc
     5821 tgcgccgaga tcgccacggc ggtctccttc tcgcgaagcg cgcgctcctt cgcggtgctc
     5881 gcctcgatga tcttctgctc gagtgcgatg ttcttcttgc gcagctcctc ggcggtttcg
     5941 gcgaccgcgg tctcggcctt ctccttcgca gcctcgacct tctgcttcgc ttcttcgacc
     6001 tcgtgcttct cggcgagcgc tgcttgctgt tgcgcctctg ccgccttctg tttggcttcg
     6061 gcggccttgc gtgcggcttc cttcgcttcc atcgcggtga gtgcgtcttg cgctttgtgc
     6121 ttctcttgct gcgcgacgac ctcggcgttg cggatccacg cagccgcgac gatcgcaccg
     6181 atcgcgacac agccgagcac ggtgaatgcg acgatcaagg cgagccgctt cgcacgcgcg
     6241 ccgcggcggg cgagggcgaa gacgtcgtcg agaaatgcct gctcgcgagc ggggagctcg
     6301 cgcggtcgga gcgcatgcca gcggcgtgct tcctccatcg cgtcgccgcg ccagagcaag
     6361 ccgtgcggcc gtcccttcgc gtcccactgt ttcgccgccg tggcgagctg ggcgaggaac
     6421 gcggcgtcct cttgatcctc gtcgagccac cgtcgcagtg ttggccagcg atcgatcagc
     6481 gattcgtgca cgatctcgac cgagcccgtg ccgccgtccg cacgcgtctg cacgacgagc
     6541 aggcgtccgg caaccagcgt atcgatcgtg cgcgcgacct cggccggatc gcccgcgagc
     6601 tgctggaggt cggcgagctc gacgatcgcg cgcgtgcgat cgggcgtgac gagccggcgg
     6661 aacatctgct gcgtgagctt ctgcgcggcc gcgttcatct gcccgaccac gtcgtcggca
     6721 tgcgtggcaa gcgcgccgct gatgccaccg atcgcggcgt agctccgcac ggtgagtaga
     6781 cgcttgtcgc gatcgcgcgc atcccacagc ttggcggcgg cgaactgcaa gagcggcaaa
     6841 ccaccgggcg tgtccgcgag cgcatcgatc atgtcgccga cgatcgcgtt gctttcgaag
     6901 tgaaacccga ccatctcgac aggagcgacg agcgcttcgc gcaaaccctc gcggtcgggt
     6961 gggcgcagga acacgaggcc gcgcgacagc tcctccatga accgcgcgtc ctcgccaacg
     7021 cgatcgagca agtccgaacg catcgcgagt acgacacgca gtggtgccgc cgtgtcgtcg
     7081 gcaacgccgg caagtgcagc cgtgaacgtg cgccgctcct cggcatccgt gacgagcgtg
     7141 tacagctcct cgaactgatc gacgaacaac aggatgtgcc cgccggattg tctcgcccgt
     7201 gcgcgcagca gcgtgccgac gtagcccggc tcggtgcgca gtcgcgtgac cagctgctcg
     7261 tgctcgccga cttcttcttt gaggtgcgag cccgatttcg tggtcagtcg ctgcacgatc
     7321 gtcgcgagtg cggcgagtgg ttggcgaccg ggacgcaacg tgacgacgtc ccactgctcg
     7381 cccgacgcct tgagagcggg accgacaccg gcgcgcacga tcgatgactt gcccacgccc
     7441 gacggaccga cgagcgcggt gatcggcaca tcacgaatcc gtgcggtgat gcgtgcgatg
     7501 tcacgagcgc ggccgaagaa gcgatgggca tcgccttcct gaaatgcggt gagccccggg
     7561 tacgggctct cggtttcggc cagggtacga gtcgcacggc ccgcgcctgg cgcgagcgct
     7621 tcgagtctgc gcacgagctc gccggcgttc gcgatgcggt ccgcgtgcgt cttgcgtaga
     7681 cactcgtcga cgagtccggc gagtgcgtcg ggtgcatcct cgacggcgct gcgcagcgtc
     7741 ggcatcggcg tatcgttgcg cgcgttcgcg aacagggctt cgccggtaaa cggctcgatc
     7801 ggatgccggc cggccagcat ctcccacatc atcacgccga gcgcccacag atccgtgcgc
     7861 tgatcgatct cgcccatgcc gagctgctcg ggcgacatat acggcaacgt gccggccatc
     7921 aggtcatggc ggctgtcctt gaccttcgcg ataccgaagt cgacgacctt gacctgaccc
     7981 gcggtcgtca cgatgacgtt ctcgggcttg aggtcgcggt ggatgatgcc gccttcgtgt
     8041 gcgcgagcga gcgcgcgcgc gattggcaac acgagctcga cgactcgcgt cgctggcaag
     8101 cgtgacgcgc caccgatcgg tcccatcatg gtgcgcagcg tctggccttc gaggaactcg
     8161 aggaccatgt acggcgtgtc gtcgagctcg tcgacctcgt gaatgatgac gatgttgtcg
     8221 tgattgcact gcgccgtcgc gcgcgcttcg gtgaggaagc gctcggcgtc ttcgcgtgag
     8281 gcctggcgca gaaatttgat cgcgacgcgg cgaccgagct tggtgtcgcg cgcggcccag
     8341 accacaccca tgccaccgcg tccgagctcg cggatcagct cgtagtgatg aacgcgctga
     8401 cccggtgacg cggcgatcgc aacacctgcg tctgcccctg gctcggattg tgtggcgtgg
     8461 aacgcctcgg gaaccgcttt ggggcgctgg gctaccggcg gtagctcggt cgcgtgtagc
     8521 gccggattcc cctcgttccc cacatcctcc agggtaacac ggtaccccag accgatcgcg
     8581 agcggcctcc ttcggttacc atgggaactg gagggtaacg tgaggtacct gtacggcgac
     8641 tcgacatcat caccgctgca gtcgaactat ctcgcgttct ttcgcgacgc gatggaattc
     8701 tgggttcatc tgctgctcgc gcagcagcgg atcaacaagc ttcgcgacga acgttccgca
     8761 accgaccagt acgccgcagc ggagcggacg cggatcggag ggttgaaggc actcgtgacc
     8821 gatgcggccg aggaggcgaa ccacgatggc gccgagtcga tgtcgctccg tgcggtcaag
     8881 agtgtcatca ccgcctcgga ccaagcgatc gcggtgatcc tcgacgagct gcagcgaaag
     8941 ctcgatgccg atcgagccgc gctggctgcc aaagaccgcc acgagcgcga cgagtgtctc
     9001 gacgcgttcg cgaagtacgt cgcgctccac gaggcgcacg agggtacgtg gcgactcaaa
     9061 gcgtacctcg acgacgatgg cagctatcaa ggtgagacat cgggggctgc cccgtacggc
     9121 gcggagtggc gatgtgcgat cgagctccag tccgatcatc caatggcgaa gggcatcaag
     9181 gtcggcgacc tcgtcaccca gatcgagctc ccacttcccg aaggatcagg gtggctcaag
     9241 aagggcaagc tgaagccgca gcgaatcgac cagtacagca tcgactcgtt tgaaaccgac
     9301 ggcacgcagt tcagctacgc gcttcgggcc accccgcgcg caccgaccgg ccttgacgtc
     9361 acgatccgcg gcgacaagat cgaggtcacg ctcgcggatg cgaaggacgc gacctcggtc
     9421 gaggtcaacc tcgacaatcg cgagcggcta ctcacgttgc gcgatcgcat cctcgacgcg
     9481 ctcacctccc atgccggggt tcgtcgccgc gtcgtgaccg ccactatcga tgacaagcca
     9541 ctcgccgacg gcgacgatct cggcgacgtc gtgaagcgca tgatcgacgc tgccacaccg
     9601 atcgtgaagc agatccgcaa gcactcgctg tcgccgcacg agctggtgat tcgccgcctc
     9661 ctcggcaacg accgtcgcga ggagatcttc gtgtcgacgg cgtcgttgct cgagaagctg
     9721 gggcgactgc cgaggaacct gcgctcgctg ttcgaaccgc tgggcctcga gtggcagcgc
     9781 gcgcacacgc cggtccccgg cgaaatcttc gtcgagccac cacggccgac ggcgcccgaa
     9841 cccgagcctg cgaaggtcga ggttgccaac gccgagcccg agcggcagca gagcgagacc
     9901 accgagtccg cgccatcgga accgtctcga gcaagcgtgc ccgagcccgt ggacgacgct
     9961 gcgggatcgg ctgcgggatc gatcgaagcc gcactgttgc gaacgtcgtc gccgagcatc
    10021 gaggtcgctc cggtggcagc ggcatctgtg ccgtcaccgc ccgttacgtc cacgatctcg
    10081 atcgatgcgc acagcaagga agcactggcc gctacggtca agcgcatcgt cggtatggca
    10141 cgcgaaggcc gtacgcccga agcgtacgcg gcctatgccg ccctcttcga ggacaacggc
    10201 ttcgcgcaac agcggccaca ggaccagcgt caggttctca agctcgtgct gatgtcgaag
    10261 accccgccgc cgcgcagcga atcggtcact cacgcgtatc gcagcgcgct cgctcggctt
    10321 cagagcctga gtgccgacag cagggatccg atcgacgacg agatgatcgg cgtgtgcctc
    10381 gcgctgctcg gctgagctgc gcgtcatctg atccgcgccg tgctacacgc agccgtatga
    10441 agaagagcgc gccggcggca ttgcgtaacc gcgaaccgat tcgggaggtg ctcgcgcagc
    10501 agctgcccga gaccgggacc gtgctgacga tcgcggaggg gtcgggcgag catgtcgtgc
    10561 acttcgctcg tagctttccc gcactgacct ggcaaccctc cgacgtcgac gccgaggcgc
    10621 tcgccagcat cgccgaatgg cgggaggaga ccgcgttgcc aaatctggcg agtccggtcc
    10681 agctcgacgt gaccgcggcg caatggccga tcgcgcacgc ggacgtcatt acgtgcatca
    10741 acatgatcca catcgcgccg tgggaggcga cgctgggtct gttcgcaggc gcggcgagaa
    10801 cactgccgtc gggtgcactg ctgtacctct atggccctta tcgtttcgac ggcgcattca
    10861 ccgcgccatc gaacgaagca ttcgacgcct cgttgcgtgc gcgtgattcg cgctggggtg
    10921 tccgcgatgt aagtgatctg cagaccgccg ccaccggttt cgtccttcgt gcggtcgtcg
    10981 agatgccggc aaacaaccat tcgctggtgt tccgccgcaa ctagctgtca gttcttcgac
    11041 ggcatccgcc cgaccacgcg cggttacgtc gcggcccgga cgttgctcca tctgcttcct
    11101 catgcggggc aaagcacttg gcgttctggt ggcgacggtt ctgattcccg tgtcggcacg
    11161 tgccgacatc tcgccgcgtg gggtgttgtc ggcgggaatc gatatcggcc accacgatca
    11221 tcccgacgac aatgggacgt ccatcggcgt caacctcgac ggcgagctcc acgtcggcat
    11281 gttcctcgtg ggtgcctcga tcggttacga cgactacgca ccgctaaacg gcgtcaccgt
    11341 gcatgccggt acgtacgcgg cacgcgtggg cgtcgacgtg ccatttgcga aaacggcgcc
    11401 gaatcgtaag ggccgcacga tccagttcga cgcg
//
LOCUS       DASUFX010000400         8702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1314453, whole genome shotgun sequence.
ACCESSION   DASUFX010000400 DASUFX010000000
VERSION     DASUFX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314453"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFV99_15980"
     CDS             complement(<1..577)
                     /locus_tag="VFV99_15980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5060867.1"
                     /translation="MRIDLPFRQLVDAAPDGMVVCDQSGTIVLVNAETERMFGYARDE
                     LIGKRIEALIPERLRARHEGHVSGFASAPRLRPMGSGLELHGRHKDGHEFPVEISLSP
                     IKSDDLMLFTAGIRDVSDRRVLERQARRANAYLTSAVESVTDAFALFDEQDRVMMMNS
                     SARHLLGAVRDSGVVGLTFESVLDNALNNAVF"
     gene            complement(567..1670)
                     /locus_tag="VFV99_15985"
     CDS             complement(567..1670)
                     /locus_tag="VFV99_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060868.1"
                     /translation="MTGRAVLVVDDNAENRALAEATLEDEGYRVVLANDGEQAVAAFT
                     RESPECILMDIRMPVLDGIGACERIRALPGGEAVAIIFLTAQRDVETFDRALAAGGDD
                     FITKPFRPSELVVRVQTALRLRRIAAERSELFAQIKQQRDQLQRLQLQKEQFAAFLVH
                     DLKNPVNSIELQAQIVARDATAAERTKRAAGSIQQETRALLRMITNLLDIAKADEGRL
                     APTRQGVDAEKLIRTVFDELAPRAASAEVTLETDASGTELMVDPDLMHRVVANLVENA
                     IRHAPDGTAIKVSATRADGHIELRIADAGPGVPAEARDKIFERFESGSTGRLNRGLGL
                     AFCKIAIEAHGGRIWVEDGSPGAVFVMRFPDAH"
     gene            1832..3538
                     /locus_tag="VFV99_15990"
     CDS             1832..3538
                     /locus_tag="VFV99_15990"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEX5060869.1"
                     /translation="MKQTFLLACVLLFACGPAGRDGNNNNGDDDDGATCPRCSDDKMS
                     VIDCNGNATACPPDQGCSNAQCMPACEAAEKNHASIGCDYYGVDMDAAMGPPMDACYT
                     MFVANTSQGQAHMNISFNGSFIDLSRFAKIPQGQGMSLQYGAYDPGQGLAPGQVAIVF
                     LGYAPGGGGPLMPNVACPVPAAIGTEAQISGNGIGHAFHLETDMPVVAYQMLPYGGGA
                     AAATGASLLLPTSAWSNNYVAVTAYDTEGSPPPISVPQGPSMNIVAMEDDTTITMRPK
                     SAIGGGGGIPAATANGTWEMKLNKGEHAQITQFAALSGSPIQSDKPVGVFGGHQIMSI
                     DRCCGDHGEQMIAPVRALGYEYVAAPHPERKPVSEVHIYRIFGAVDGTALTYDPPNVG
                     PDKVELGQFLEIRSADPFTVRSQDQDHPFLMFTYMSGAGEQGEGGWGDADFVRVVPPA
                     QYMKHYVFFADPTYPFTGLTVVRKKKDGAFKDVKLACSGALTGWHPVGSGDFEITTVK
                     LVDHWAGQGGCNNGVNEMDSEAGFGVWVWGWGSEDTNTGWVSYGYPAGEGVLPINDVV
                     IL"
     gene            3549..4373
                     /locus_tag="VFV99_15995"
     CDS             3549..4373
                     /locus_tag="VFV99_15995"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5060870.1"
                     /translation="MTVWRVVTLNIWNRQGPWAQRLPLIREGLRALDADVIGLQEVLG
                     FPGMPSQADEIAAGLGWHVYYAPAWHIGGGLTYGNAIVSKHPLVDGACLPLPSPPGLD
                     TRTVAFARVELPHGPMPVFVTHLTFQLHLGHARCEQVRALVEHVKTLAPVDGPPPVLL
                     GDFNADPDSDEMRFLRGLTPLGGTSVYFADCWSATGNLEPGYTYDRRNTYALRSREPS
                     RRIDYIYVRGPDRSLRGEPLSARLALTEPVDGVWASDHFAVVAEIHAAARAHDPGL"
     gene            complement(4370..5356)
                     /locus_tag="VFV99_16000"
     CDS             complement(4370..5356)
                     /locus_tag="VFV99_16000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5060871.1"
                     /translation="MEPRIAIEARETAAGARRQLVTCAPILGELARRLRAAPPPLVVT
                     CARGSSDHAATYGKYVLETMAGNLVASVGPSVASMYHRAPVGLTNALFIAVSQSGKSP
                     DVLKLTDAAKRGGALVVGIVNDEASPLAGHCDLVLPICAGEERAVAATKSFLLSGLAF
                     VQLAAAWTGDATLVDAVARAPDAFDAACEAPWDLTPLATATGLYVVGRGVGLGAAQEL
                     ALKLKETCRLHAEAFSTAEVLHGPVALVRDGFPIVAIEQADQTAAATRDVLAKLAALG
                     ATVHRCPPVDAPALLAPLCQVQSFYLGVSALAAARGLDADAPAHLTKVTETL"
     gene            complement(5380..6222)
                     /locus_tag="VFV99_16005"
     CDS             complement(5380..6222)
                     /locus_tag="VFV99_16005"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5060872.1"
                     /translation="MIRALFALAIALATACGGSKPQCAVVAPSIPGPAFLWKVQKGGD
                     VVWLFGTIHNTGLDAVPASAKDALAKSPRLVTELGDVDVDTDMFRKYARNASGKGIDQ
                     LLPADDWWDLRDALLGKVKEDDLRRMRPWYAMSQLTKFLAPSQGPSMDVELAERAEKL
                     SIPVEPLESWEDQLWMLDRTVGVADLQEAIRARKTMRCDLARLENAYRAGDIVVMQAL
                     LVVPRTKDVMLTARNKKWFPALEKQFGQGGAFVAVGLGHLLGDDGLVAMLQRAGYTVE
                     RIAQ"
     gene            complement(6219..7595)
                     /locus_tag="VFV99_16010"
     CDS             complement(6219..7595)
                     /locus_tag="VFV99_16010"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5060873.1"
                     /translation="MVGFVFAIGCGTDPATGDDDGVVFPYPELPADTQRPGDAAKGYD
                     FLINGGYITCGIPKSIAPSGSAADRMAGRTGDNAALPYYWSAATSAEGVRVVSANCMF
                     CHAGKINDQLVIGLGAADRDFTGDQVALLDTALGLVSGVEKTELERFRDRMAAIADYS
                     RTITIGVNPADSLTAALFSHRDPATLAWSAEPMIPLPPAIVPPVDVPPWWRMSKKHTM
                     FYNAAGRGDHARIMMAASLLCTENVAEAQMIDQAFVDVRAWIESSVQPPAWPFGAVDT
                     QLVAKGKTVFEATCAKCHGTYGEGGVYPNQLVPLADIGTDSTLVEGENQFAGAFVEWF
                     AASFWGQTSRLEPSIGYVAPPLDGVWATAPFLHNGSVPTIEALLDSSKRPKYWTRTFQ
                     STDYDPATLGWKFTKLDHGHAGEPTAGARVKIYDTTQAGYGNGGHTFGDDLSDSERKA
                     VLEYLKTL"
     gene            7649..8323
                     /locus_tag="VFV99_16015"
     CDS             7649..8323
                     /locus_tag="VFV99_16015"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX5060874.1"
                     /translation="MVLLNLDAGEHADEPEELWALADVLSVACGGHAGDEQSMARVVA
                     FSVGRARPRIGAHPSYPDRLGFGRRSLAVDPQALRQTIAWQCAQLAKVAAAHGQRVQW
                     VKPHGALYHDAAKDLAAAGAVIFGAIEALGPDVIVIGPPGGVLYEASRQAKLSYLREG
                     FADRATNPDGSLVPRDQPGALITDPVVAAARARELVGDVDTICVHADTPGALAIARAV
                     REALDA"
     gene            8316..>8702
                     /locus_tag="VFV99_16020"
     CDS             8316..>8702
                     /locus_tag="VFV99_16020"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX5060875.1"
                     /translation="MPEWRALGDRAIRFPRPAVSARALVEAVHKWPGVVDVVVARNDV
                     AAYFDREPVIDPAELAALALLRDVDGAARDIAIRVVYDGPDLDDIARAKQLTVAEIQR
                     LHAQATYTVDTIGFAPGFAYLTGLDTH"
BASE COUNT         1428 a         2915 c         2886 g         1473 t
ORIGIN      
        1 cgaagaccgc gttgttgagc gcgttgtcga ggaccgactc gaacgtgagg ccgacgacac
       61 cgctgtcccg cacggcgccg agcaagtgcc gcgccgagct gttcatcatc atcacgcggt
      121 cctgctcgtc gaacagcgcg aacgcatcgg tgaccgactc gaccgccgac gtcaggtagg
      181 cgttggcgcg acgcgcctgg cgctcgagca cgcgccggtc ggagacgtcg cgaattccgg
      241 cggtgaacaa catgaggtcg tcgctcttga tcggcgacag gctgatctcg accggaaact
      301 cgtgaccgtc tttgtgccgg ccgtgcagct cgaggcccga gcccatcggc cgcaggcgcg
      361 gcgccgacgc gaagcccgat acgtgacctt cgtgccgggc gcgcagtcgc tccgggatca
      421 gggcctcgat gcgcttgccg atgagctcgt cgcgcgcgta cccgaacatg cgctcggtct
      481 ccgcgttgac gagcacgatg gtgcctgact gatcgcacac gaccatgccg tcgggtgcgg
      541 cgtcgacgag ctggcggaat ggcaggtcaa tgcgcatcgg ggaacctcat gacgaacacc
      601 gcacccggcg agccatcctc gacccagatg cggcctccgt gagcctcgat cgcgatcttg
      661 cagaacgcaa gaccaaggcc gcgattcaag cgcccggtcg agccactctc gaagcgctcg
      721 aagatcttgt cgcgcgcctc ggctggcaca cccgggcccg cgtcggcgat gcgcaactcg
      781 atgtggccgt cggcgcgcgt cgcgctgacc ttgatcgcag tgccatccgg cgcgtgacgg
      841 atcgcgttct cgacgaggtt cgcgaccacg cgatgcatga gatccggatc gaccatcagc
      901 tcggtcccgc tcgcatcggt ctccagtgtc acctcggcgc tcgcagcgcg cggcgcgagc
      961 tcgtcgaaca cggtgcggat cagcttctcg gcgtcgacac cttgtcgcgt cggagcgagc
     1021 cgcccttcgt cggcctttgc gatgtcgagc aggttcgtga tcatgcgcag gagtgcgcgc
     1081 gtctcttgct ggatgcttcc cgcagcacgc ttcgtgcgct cggcggcggt cgcgtcccgt
     1141 gcgacgatct gcgcctgcag ctcgatcgag ttgaccgggt tcttcaggtc gtgaacgagg
     1201 aacgcggcga actgctcctt ctggagctgt aaccgctgga gctgatcgcg ctgctgcttg
     1261 atctgcgcga aaagctcgct gcgctcggca gcgatccggc gcaagcgcag cgcggtctgc
     1321 acgcgcacga cgagctcact cgggcgaaat ggcttcgtga tgaaatcgtc gccgcctgca
     1381 gcgagcgcgc gatcgaacgt ctcgacatcg cgttgcgcgg tgagaaagat gatcgcaacg
     1441 gcttcgccac cgggcagcgc gcgaatgcgt tcgcatgcgc cgatgccgtc cagcaccggc
     1501 atgcggatgt ccatcaagat gcactcggga gactcgcgcg tgaaggcagc gaccgcttgc
     1561 tcgccatcgt tcgcgagcac gacgcgatat ccctcgtcct cgagcgtcgc ttctgcgagc
     1621 gctcgattct cggcgttgtc gtccacgaca aggaccgctc taccagtcat tgtccgagcc
     1681 tccgccatgt accgaccatg gcacgtgttg cgatgtcgcg tgcgcgcgac gtgcaaactt
     1741 tcgcacagca caaatcctcg acgagaacag cgttcgtttt tcgctatctg cgtctgtacg
     1801 cgctgtgtag tgtgcgcagc cagaggggat catgaagcag acgtttttgc tcgcgtgtgt
     1861 tctgttgttc gcgtgtggcc cggcggggcg cgacggcaat aacaacaacg gtgatgacga
     1921 cgacggcgca acgtgtccgc gttgctccga cgacaagatg agtgtcatcg attgcaatgg
     1981 caatgcgaca gcttgtccac ccgaccaagg gtgctcgaac gcgcaatgta tgccggcatg
     2041 cgaggcggcc gagaagaacc acgcgtcgat cggctgcgac tattacggcg tcgacatgga
     2101 tgccgcgatg gggccgccga tggacgcttg ctacacgatg ttcgtagcga acacgtcgca
     2161 aggccaagcg cacatgaaca tctcgttcaa cgggtcgttc atcgatctgt cccggttcgc
     2221 gaagatcccg cagggccagg ggatgtcact tcagtacggc gcctacgatc cgggtcaagg
     2281 cctcgcgccc ggtcaggtcg cgatcgtctt cctcggctat gcgccgggcg gcggtggtcc
     2341 gctgatgccg aacgtcgcgt gcccggtacc ggcagcgatc ggcaccgagg cgcagatctc
     2401 gggtaacgga atcggtcacg cgttccacct cgagaccgac atgcccgtcg tcgcgtatca
     2461 gatgctgccg tacggcggtg gcgccgcggc ggcaaccggt gcgtcgctgc tgttgccgac
     2521 gagcgcgtgg tcgaacaact acgtcgcggt caccgcgtac gacaccgaag gttcgccgcc
     2581 gccgatctcg gtgccgcagg gtccctcgat gaacatcgtc gcgatggaag acgacaccac
     2641 gatcacgatg cgtccgaaat cggcgatcgg cggcggtggt ggcattcccg cggcgacggc
     2701 gaacggcacg tgggagatga agctcaacaa gggcgagcac gctcagatca cccagttcgc
     2761 cgcgctgtcg ggtagcccga tccagtcgga caaaccggtc ggcgtgttcg gcggtcacca
     2821 gatcatgtcg atcgatcgct gctgcggcga tcacggcgaa cagatgatcg cgcccgtgcg
     2881 cgcactcggc tacgagtacg tcgccgcgcc gcatcccgag cgcaagccgg tcagcgaggt
     2941 ccacatctac cgcatcttcg gcgccgtcga cggcaccgcg ctcacctacg atccgccgaa
     3001 cgtcggcccc gacaaggtcg agctcggcca gttcctcgag atccgcagcg cagatccgtt
     3061 caccgtgcgc agccaggatc aagatcatcc gttcttgatg ttcacgtaca tgagcggcgc
     3121 cggcgagcaa ggcgaaggcg gttggggcga cgctgacttc gtgcgcgtcg tgccgcccgc
     3181 gcagtacatg aagcactacg tcttcttcgc cgatccgacg tatccgttta ccggcctcac
     3241 cgtcgtgcgc aagaagaagg acggtgcgtt caaggacgtc aagctcgcgt gctcgggcgc
     3301 actcaccggc tggcacccag tcggctccgg cgacttcgag atcacgacgg tcaagctcgt
     3361 cgatcactgg gcaggccagg gcggctgcaa caacggcgtc aacgagatgg atagcgaagc
     3421 cgggttcggc gtctgggtgt ggggctgggg cagcgaggac acgaacaccg gctgggtcag
     3481 ctacggctat cccgctggcg agggcgtact gccgatcaac gacgtcgtga ttctgtaatg
     3541 tagagggcgt gaccgtctgg cgagttgtca cgctcaacat ctggaatcgg cagggcccgt
     3601 gggcgcagcg cttgccgctg atccgcgaag gtctgcgcgc actcgacgcc gacgtgatcg
     3661 gcctgcagga ggtgctcggg tttccgggca tgccgagcca ggccgacgag atcgcggcgg
     3721 gcctcggctg gcacgtgtac tacgcgccgg cgtggcacat cggcggtggg ctcacctacg
     3781 gcaacgcgat cgtgtcgaag catccgctcg tcgacggcgc gtgcctgccg ctaccatcgc
     3841 ctcccggtct cgatacgcgc acggtcgcgt tcgcgcgcgt cgagctgccg cacgggccga
     3901 tgccggtgtt cgtcacgcac ctgacgttcc agctccatct cggccatgcg cgctgcgaac
     3961 aagtgcgcgc gctcgtcgag cacgtgaaga cactcgcgcc cgtcgacggc ccgccacccg
     4021 ttctgctcgg cgacttcaac gccgatcccg attccgacga gatgcggttc ttgcgtggcc
     4081 tcacgccgct cggtggcacg tcggtgtact tcgccgattg ctggagtgcg accggcaatc
     4141 tcgaaccggg ctacacgtac gatcggcgca acacgtacgc actgcgctcg cgcgagccat
     4201 cgcggcgcat cgattacatc tacgtgcgcg gtccagatcg ttcgctgcgc ggcgagccac
     4261 tgtccgcgcg actcgcactg accgagcctg tcgacggagt gtgggcgtcg gatcacttcg
     4321 ccgtcgtcgc cgagattcat gctgcggcac gcgcgcacga tccggggctt tagagcgttt
     4381 ccgtgacctt cgtcaggtgc gccggcgcgt cggcgtcgag gccacgtgca gcagcgagcg
     4441 ccgacacgcc gaggtagaag ctctgcacct gacacagtgg tgcgagcagt gcgggcgcgt
     4501 cgaccggtgg gcagcgatgc accgtcgcgc cgagcgcggc cagcttcgcg agcacgtcgc
     4561 gcgtggcggc tgccgtttga tcggcttgct cgatcgcgac gatcggaaat ccatcgcgca
     4621 cgagcgcgac cggaccgtgc agcacttcgg cggtgctgaa cgcttcggca tgcagccggc
     4681 acgtctcctt gagcttgagc gcgagctcct gcgcggcgcc gagcccgacg ccacggccga
     4741 cgacgtagag accggtcgcg gtggcaagcg gtgtgagatc ccacggcgcc tcgcatgcgg
     4801 catcgaatgc atcgggcgcg cgggcaaccg catcgacgag cgtcgcgtcg ccggtccacg
     4861 cggcagcgag ctggacaaat gcgagtcccg agagcaagaa cgacttcgtg gccgcgaccg
     4921 cgcgctcttc gcccgcgcag atcggcagca cgaggtcgca gtgcccagcg agcggcgatg
     4981 cctcgtcgtt gacgatgccg acgacgagtg cgcctccacg ctttgctgcg tcggtcagct
     5041 tcagcacgtc cgggctcttg ccgctctgcg agaccgcgat gaacagcgcg ttcgtcaggc
     5101 cgaccggcgc gcggtgatac atcgatgcca ccgacggacc gaccgacgcg accaggttgc
     5161 ctgccatcgt ctcgagcacg tacttgccgt acgtcgccgc gtgatccgag ctgccgcgcg
     5221 cacaggtgac gaccagcggt ggcggcgcag cacggagccg ccgcgcgagc tcgccgagga
     5281 tcggggcgca ggtcacgagc tggcggcgtg cacctgccgc ggtctcgcgg gcctcgatcg
     5341 cgatcctcgg ctccataccc aagcgtagcg tgcgtttgcc tactgcgcga ttcgctcgac
     5401 ggtgtaaccc gccctctgca gcatcgccac gagcccgtca tcgccgagca gatggccgag
     5461 cccgaccgcg acgaacgcac cgccctgacc gaactgcttc tcgagcgcgg gaaaccactt
     5521 cttgttgcgc gcggtcagca tcacgtcctt cgtgcgcggc acgaccaaca gcgcctgcat
     5581 caccacgatg tcgccagcgc gataagcgtt ctcgaggcgc gcgaggtcgc agcgcatcgt
     5641 cttgcgcgcg cgaatcgctt cctgcagatc ggcgacgccg accgtgcggt ccagcatcca
     5701 gagctgatct tcccagctct cgagcggctc gaccgggatc gagagcttct ctgctcgctc
     5761 ggccagctcg acgtccatcg agggcccttg cgacggcgcg agaaacttcg tcagctgcga
     5821 catcgcgtac cacggccgca tgcggcgcag atcgtcttcc ttcaccttgc cgagcagcgc
     5881 gtcgcgcagg tcccaccagt cgtcggcggg cagtaactga tcgatgcctt tgcctgatgc
     5941 attgcgcgcg tacttgcgga acatgtctgt atcgacgtcg acgtcaccga gctcggtgac
     6001 aaggcgaggc gactttgcga gcgcgtcctt cgccgaggcg ggcaccgcgt cgagacctgt
     6061 gttgtggatc gtgccgaaca gccagaccac gtcgccgccc ttctgcactt tccatagaaa
     6121 cgccgggccg ggaatcgacg gcgccacgac cgcgcactgt ggcttcgagc caccgcatgc
     6181 ggttgccagc gcgatggcga gcgcaaacag cgcgcgaatc acagcgtctt caggtactcg
     6241 agcactgcct tgcgttcgct gtcgctcaga tcgtcgccga acgtgtggcc gccgttaccg
     6301 tagccggcct gcgtcgtgtc gtagatcttc acgcgcgcgc cagcggtggg ctcgccggcg
     6361 tggccgtgat cgagcttcgt gaacttccag ccgagcgtgg cgggatcgta gtccgtcgac
     6421 tggaatgtgc gcgtccagta cttcggccgc ttcgacgaat cgagcagcgc ttcgatcgtc
     6481 ggcaccgagc cgttgtggag gaacggcgcg gtcgcccaga cgccatcgag cggtggcgcg
     6541 acgtagccga tcgacggctc gagtcgcgac gtctggcccc agaacgaggc ggcgaaccac
     6601 tcgacgaacg cgccggcgaa ctggttctcg ccttcgacga gcgtcgagtc cgtaccgatg
     6661 tcggctagcg gcaccagctg attcggatac acaccgcctt cgccgtacgt gccgtggcac
     6721 ttcgcgcacg tcgcctcgaa cacggtcttg cccttcgcga cgagttgcgt gtcgaccgcg
     6781 ccgaacggcc acgcaggcgg ctgcacgctc gactcgatcc atgcacgtac gtcgacgaac
     6841 gcctgatcga tcatctgcgc ctcggcgacg ttctcggtgc acaacagcga tgccgccatc
     6901 atgatccgcg cgtgatcgcc gcggccggcc gcgttgtaaa acatcgtgtg cttcttggac
     6961 atgcgccacc atggcggcac gtcgacgggc ggcacgatcg cggggggcag cggaatcatc
     7021 ggctcggccg accatgcgag cgtcgccgga tcacggtggg agaacagcgc ggcggtcaag
     7081 ctgtcggccg ggttcacacc gatcgtgatc gtgcgcgagt agtcggcgat cgccgccatg
     7141 cgatcgcgga agcgctcgag ctcggtcttc tcgacaccac tgacgagacc gagcgcggtg
     7201 tcgagcagcg cgacctgatc gccggtgaag tcgcggtcag cggcgccgag gccgatcacg
     7261 agctggtcgt tgatcttgcc cgcgtggcag aacatgcagt tcgccgacac gacgcgcacg
     7321 ccctccgccg atgtcgccgc actccagtag tagggcagcg ctgcgttgtc gccggtacgg
     7381 ccggccatcc gatcggctgc agagccgctc ggcgcgatcg atttcgggat gccacaggtg
     7441 atgtagccgc cgttgatcaa gaagtcgtag cccttcgccg cgtcgccagg ccgctgcgta
     7501 tcggccggta gctccggata cgggaagacg acgccatcgt cgtcaccggt ggccggatcc
     7561 gtgccacacc cgattgcgaa gacgaatccc accacggcta gacaaccgaa ggtccgcatc
     7621 gcggcggacc ctacactaca atccagccgt ggtgctgctg aatctcgacg ccggcgagca
     7681 cgcggacgag cccgaggaat tgtgggcgct tgccgacgtg ctgtcggttg catgcggcgg
     7741 tcatgcgggt gacgagcagt cgatggcgcg cgtggtcgca ttctcggttg gtcgcgctcg
     7801 gccgcggatc ggcgcgcacc cgtcgtatcc cgatcggctc ggcttcggcc gtcgctcgct
     7861 cgctgtcgat ccgcaggcac tgcggcagac gattgcgtgg caatgcgcgc agctcgcgaa
     7921 ggttgccgct gcacatggtc aacgcgtgca gtgggtgaaa ccgcacggcg cgctctatca
     7981 cgatgcggcg aaggatctgg cggcagccgg tgcggtgatc ttcggcgcga tcgaagcact
     8041 cggtcccgac gtcatcgtga tcggcccgcc gggcggcgtg ctctacgaag cgagtcggca
     8101 agcaaagctc agttacctgc gcgagggctt cgccgaccgc gcgacaaacc cggatggctc
     8161 gctcgtgccg cgcgatcagc cgggtgcact gatcaccgat ccagtggtgg cggccgctcg
     8221 cgcgcgggag ctcgtcggcg acgtcgatac gatctgcgtc cacgcagata cgcccggagc
     8281 actcgcgatc gcgcgtgcgg tgcgtgaggc gctcgatgcc tgaatggcgc gcgctcggcg
     8341 atcgcgcgat tcgcttcccg cgacctgcgg tgtccgcgcg ggcgctcgtg gaagcggttc
     8401 acaagtggcc aggtgtggtc gacgtcgtcg tcgcacgtaa cgacgtcgcg gcgtatttcg
     8461 atcgcgagcc cgtgatcgat ccagccgagc tcgccgcgct cgccctgctg cgcgatgtcg
     8521 atggggccgc gcgagacatt gcgatccgtg tcgtttacga cggtccggat ctcgacgaca
     8581 tcgcgcgggc gaagcagctc accgtcgccg aaatccagcg cctgcacgcc caagcgacgt
     8641 acaccgtcga cacgatcggc tttgcaccag gcttcgcgta cctgaccggc ctcgacacgc
     8701 at
//
LOCUS       DASUFX010000401         6641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1176209, whole genome shotgun sequence.
ACCESSION   DASUFX010000401 DASUFX010000000
VERSION     DASUFX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176209"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFV99_16025"
     CDS             <1..618
                     /locus_tag="VFV99_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060876.1"
                     /translation="DAMPGPNLLVQKTAVSFTTGTRTLTLPAPSTAGSLLVITVGAND
                     ISSLTLPSGWTIAASVLTNGACATAIAYYPNNPGGITSVILQQPMLQPTAAQLSEWSP
                     ITANPLNGTSQTQGGIQATMQTAMVATIATKGVAITAFCEDVNNPTYTPGAGWTTLGT
                     SSNGSAEPSFISDYKAVTAGGIYTETVTSSVSAKYAAVIAVFASP"
     gene            704..1753
                     /locus_tag="VFV99_16030"
     CDS             704..1753
                     /locus_tag="VFV99_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5060877.1"
                     /translation="MRGTWAVLFLIACGPTGQKHPGNGGDDDDTGSGSIDAGETYFDS
                     AGNDIDASCGAQMQQIGVVNLGDPPDLLIVLDRSGSMTEPPPAFPPVFDSKWNIMKNA
                     LNSIATMKDQNIKFGLLEFPSDQNCAADANPEVAIALGAHTPMATYFAGRSPGGNTPA
                     HVALTSALSYYQTIPTNPAGRYVLFATDGLPNCLGGDPDVASDQATVQAVQALYNAGI
                     PTYVLGFGTFGLPTGVLNDAAQAGGKAKTGVTKFYEANNANDLAMALQAIAGGIIVPS
                     CTFQLQSVPPDPTNVTVTINGTAVPRSPSHMNGWDYYPNMMTITFFGSYCSQIMMGST
                     TNVSFVYGCPGPIIQ"
     gene            complement(1758..2015)
                     /locus_tag="VFV99_16035"
     CDS             complement(1758..2015)
                     /locus_tag="VFV99_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060878.1"
                     /translation="MGVKKFRDISEIEDVVYERGSRKLYEVIRYVWGLSDVICPQRFP
                     SGVFKHRTIEDAEALREQWEEANFRAHRARMARQRGDKSGT"
     gene            complement(2015..2485)
                     /locus_tag="VFV99_16040"
     CDS             complement(2015..2485)
                     /locus_tag="VFV99_16040"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX5060879.1"
                     /translation="MDVDLVQQVLAALERENARYVVFGAVALALHGLPRATEDLDLFL
                     APDRDNIERVKAALRSVFDDPHIDEISADDLLGEYPAVQYGPPEGPFHIDLLTRLGEL
                     FDFDSLESERVDFGGVTVSVVTPKMLYRMKKGTVRPKDHVDAARIAQRFSLKDD"
     gene            2616..3896
                     /locus_tag="VFV99_16045"
     CDS             2616..3896
                     /locus_tag="VFV99_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060880.1"
                     /translation="MIRSFSPRVIARGEGYLEAIDAAIDRCGGVVGSVQGSRENPYDI
                     RMRPSAEDARVLVAACSCPAAEEGACKHLWAMLRVIDREDAWPREVSVPTKLVVTDVD
                     DRDLDLIIENEAVEFCNPGTSVSARATRDLGKPRAPKVPSWRDRMDPWPHSASLPAQK
                     PANVEYIVDVRAMATLTSARRSAFPQLDERGCTLLLATTTQTKATRTRPSRTRRTFGR
                     VDSSRLSPVDVEIHQVLFGIEELSAHGWSYSSRFYYSPQARSGVRIAAERVADVLSRL
                     SATGRLGWLPDPESGLAPLGWDEQGTWILEVSMEAVAEHRVRATGCLVRGDERIPLDD
                     IAAVGGGGVAIFGTRLLHVATEGSSSAGVIGPCFACHSATNSRTACRRSSAVAASSSH
                     ADTLRPARSAAARTSSHSAGSIETLSLSTATGRA"
     gene            complement(3902..4564)
                     /locus_tag="VFV99_16050"
     CDS             complement(3902..4564)
                     /locus_tag="VFV99_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5060881.1"
                     /translation="MTAKQVGGDVAVEVARQLTEPLRALRDRLGLVVDHIERHVATST
                     GPTPYPYRSLQTLRQDLASAYLEATQLARRVEELDRALVTEEPHWFDLATAVDLGIRL
                     AGHHLGPGIELLFDLGNVAPARGIPGTLSLLVAQLVAISAHSARQAAGSSLTVRVWTE
                     DEWGVVTIADNGGGDARASEVGDVAREVLAPWGATIDALSDDGRGCAFELRLATQLVP
                     TE"
     gene            complement(4561..>6641)
                     /locus_tag="VFV99_16055"
     CDS             complement(4561..>6641)
                     /locus_tag="VFV99_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060882.1"
                     /translation="KAYLRDKYSQQEMAAERRGMQTGRALTHSGESNLIPRYSADGKE
                     LYWLEYTGYSLPKVRAMPVGGDVTTAHDVVQIDAMGEYDLLPDGSLVYEQGRQYRAVY
                     GYEDIFRWDARTRQTVRLTTGRRARDPVVSPDGRRVAYSQNEHAESSLAVMDLAPDAP
                     ATTVWRGKRYDQAYQPAWSPDGTRIAFSAWRRPGYRDILIVDVATGKIDEITNDRAID
                     IQPQWAADGSTLYFASDRTGISNIYAFDLHDRTTWQLTNVLGGAFSPAASPDGKRIAF
                     IASVPKGGYDLYELPVDRSRWLPARDFVDDRPDPVVIADDEAVVTQARPYRAMESLAP
                     QVWTLQVDTTSQSANIATNGSDAVGLHGYSMALGLDYATGDANIGASYGYFGLLPAIR
                     GAVSRTLLERGGWRVDGVPKRFKEEDWSATISVGVPFESRPSSSWTAAFDYDVDWFRL
                     VKAPDMVLDPNAHTPMHPPTDYVQAGIGSRVAFSTIRGTTFGLGSQSGFDGSVAIRFD
                     HPAIGAKYRNVTFSYAADRFQKLWGDTPVLSARLVGAFRVGDLPRAGSYGLGGVPPQD
                     VANSIVNSLRSASSGYLRGYPARTVAGNQFHLLNLEYRQELWNVERGLQTLPVYFRRL
                     HFALLSDTGVAFDTTFDANRDLRTSVGAALRLDAFFGYFVPGTFEIGYSRGLTDEGIN
                     ETWFLLTGSL"
BASE COUNT         1173 a         2185 c         2196 g         1087 t
ORIGIN      
        1 gacgccatgc ccgggcctaa cctgctcgtc cagaagaccg cggtgagctt cacgaccgga
       61 acgcggacgc tgacgctgcc cgcgccgagt accgcgggct cgctgctcgt gatcacggtc
      121 ggtgcgaatg acatctcgag cctgacgttg ccgagcggct ggacgatcgc ggcatccgtg
      181 ctgacgaacg gcgcgtgcgc gaccgcgatc gcgtactacc cgaacaaccc cggcggcatc
      241 acgagcgtga tccttcagca gccgatgctg cagccgacgg cggcacagct ctccgagtgg
      301 tcaccgatca cggcgaaccc gctgaacggc acgagtcaga cgcaaggcgg tattcaggcg
      361 acgatgcaga ccgcgatggt ggcgacgatc gcgaccaaag gcgtcgcgat cacggcgttc
      421 tgcgaggacg tgaacaatcc gacgtatacg cccggtgcgg gctggacgac cctcggcacc
      481 tcgtcgaacg gttcggccga gccgtcgttc atcagcgact acaaggccgt gacagccggc
      541 ggcatctaca ccgagacggt cacgagcagc gtcagcgcca aatacgcggc ggtgatcgcc
      601 gtattcgcct cgccgtagtg cgcgagtagc gcagcgggct gcacgagcgg attgcacttg
      661 gtgtcgttgc tcgtgcacgg cgttgcgtga gcccctctag gtcatgaggg gaacctgggc
      721 cgtcctgttt ttgatcgcgt gcggaccgac cgggcagaag catcccggta acggcggaga
      781 tgacgacgac accggcagcg gatcgatcga tgccggcgag acgtacttcg attccgcggg
      841 taacgacatc gacgcgtcgt gcggcgcgca gatgcaacag atcggcgtcg tcaatctcgg
      901 cgatccgccc gatctgttga tcgtgctcga tcgctcgggc tcgatgaccg aaccgccacc
      961 cgcattccca ccggtgttcg actcgaagtg gaacatcatg aagaacgcgc tgaattcgat
     1021 cgcgacgatg aaggaccaga acatcaagtt cggtctgctc gagttcccga gcgatcagaa
     1081 ctgcgcggcg gatgcgaatc ccgaggtcgc gatcgcgctc ggcgcgcaca cgccgatggc
     1141 gacgtacttc gcgggtcgtt cgccgggcgg caacacgccg gcgcacgtcg cgctgacgag
     1201 cgcgctttcg tactaccaga cgattccgac gaaccccgcc gggcgctacg tgctgttcgc
     1261 gaccgacggc ttaccgaatt gcctcggcgg cgatccggac gtggcgagcg atcaggcaac
     1321 cgtgcaggcc gtgcaggcgc tctacaacgc cggcattccg acctacgtgc tcgggttcgg
     1381 cacgttcggc cttcccaccg gcgtgctcaa cgatgccgcg caagccggcg gcaaggcgaa
     1441 gacgggcgtg acgaagttct acgaggcgaa caacgcgaat gacctcgcga tggcgctgca
     1501 ggcgatcgcc ggcggcatca tcgtgccgtc gtgcacgttc cagctgcaga gcgtgccgcc
     1561 cgatccgaca aacgtcaccg tcaccatcaa cggcacggcg gtgccgcggt cgccgtcgca
     1621 catgaacggc tgggactact acccgaacat gatgacgatc acgttcttcg gcagttactg
     1681 cagccagatc atgatgggtt cgacgacgaa cgtcagcttc gtctacggtt gcccgggccc
     1741 gatcattcag tagccgatca ggtgcccgat ttgtcgccgc gttgtcgtgc catccgtgcg
     1801 cggtgcgcgc gaaagttggc ctcctcccac tgctcgcgca aggcctccgc gtcctcgatc
     1861 gtccggtgct tgaaaacgcc cgatgggaat cgctgcggac agatcacgtc cgagaggccc
     1921 catacgtaac ggatcacttc gtacagcttg cgactgccgc gctcgtacac gacgtcttcg
     1981 atctcggaga tgtcacggaa cttcttgacg cccatcagtc gtccttcagg ctaaaccgct
     2041 gcgcgatgcg cgcggcatcc acgtgatcct tcggtctgac cgtccccttc ttcatccggt
     2101 acagcatctt cggcgtcacg accgagacag tgacgccacc gaagtcgacg cgctccgact
     2161 cgagactatc gaagtcgaag agctccccga gccgggtcag cagatcgatg tgaaacggtc
     2221 cttcgggtgg gccgtattgc acggcaggat actctccgag cagatcatcg gccgagatct
     2281 cgtcgatgtg agggtcgtcg aacacagatc ggagcgcggc cttcacacgc tcgatgttgt
     2341 cgcgatcggg cgctagaaaa aggtccagat cctccgtggc acgcggaaga ccgtgcagtg
     2401 ccagcgcgac cgcgccgaac accacatagc gtgcgttctc acgctccaac gcggccagca
     2461 cctgctggac gagatcgacg tccattcgaa gacagcgtag cgcacgacca agggaagtgg
     2521 ctacctatcc ctttgtcagt acgtcgagct agaaccagcg agcaatgccc gccgtcggct
     2581 ccgagtccag cgtgcgccgc aacctcgcac gagagatgat tcggtcgttc tcaccgcgcg
     2641 taatcgcgcg cggtgaaggc tatctcgagg cgatcgacgc tgcgatcgat cgctgcggcg
     2701 gcgtcgtcgg tagcgtccag ggttcgcggg agaaccccta tgacattcgc atgcgcccgt
     2761 cggccgaaga cgcgcgcgtg ctcgtagcag cgtgctcgtg cccggccgcc gaggaaggcg
     2821 cttgcaagca tctatgggcc atgttgcgcg tcatcgatcg ggaggatgca tggccacgcg
     2881 aggtctccgt accgacgaag ctcgtcgtca ccgacgtcga cgatcgtgat cttgatttga
     2941 tcatcgagaa cgaggcggtg gagttctgca atccgggcac gagcgttagc gcgcgggcga
     3001 cccgcgatct cggcaagcca cgtgccccga aggtcccgag ctggcgcgac cgcatggatc
     3061 cctggcctca cagcgcgtcg ttacctgcgc agaagccggc aaacgtcgag tacatcgtcg
     3121 acgtgcgcgc gatggccaca ctaacgtccg cgcggcgctc ggcgttcccc cagctggacg
     3181 agcgtggctg cacgctgctg ctcgcgacga ccacgcagac caaggccacg cgcacgcggc
     3241 cgtcgcggac gcgccgaacg ttcggcaggg tcgactcgtc gcggttaagc cccgtcgacg
     3301 tcgagatcca tcaagtgttg ttcgggatcg aggagctatc cgcgcacggc tggtcataca
     3361 gcagccgctt ctattactct ccgcaggctc gttccggcgt gcgaatcgca gctgagcggg
     3421 tcgcagacgt gttgtcccga ctctctgcaa ccggtcgatt ggggtggctg ccggatcccg
     3481 aaagcggact cgcgccgctc ggctgggacg agcagggtac gtggattctc gaagtaagca
     3541 tggaggcggt agccgagcat cgcgtgcgcg cgaccggctg cttggtgcgt ggcgacgagc
     3601 gaatcccgct cgacgatatt gcggcggtcg gtggcggcgg tgtcgcgatc ttcggcaccc
     3661 ggctgctcca cgtcgcgacc gaaggatcca gctcggccgg cgtgatcggc ccgtgcttcg
     3721 cctgccactc ggcaacgaac tcgcgcactg cctgtcgacg aagctcagcc gtggcagcga
     3781 gttcaagcca cgcagatacg ctgaggccag cgcgttccgc ggcggcgcgc acatcctcgc
     3841 acagcgcggg atccatcgag acgctgagcc tttcgacggc caccggccga gcttagcgcg
     3901 actactcggt aggaacgagt tgcgtcgcga gtcggagctc gaacgcgcag ccacggccgt
     3961 cgtcggaaag cgcgtcgatc gtcgcacccc acggcgcgag cacctcgcga gcgacatcac
     4021 cgacctcgct cgcccgcgca tcgccgccgc cgttgtcagc gatcgtgacg acgccccact
     4081 cgtcttccgt ccacacgcgc acggtcagcg agctgcccgc ggcctggcgc gccgaatgcg
     4141 cgctgatcgc gacgagctgc gcgacgagca gagacagcgt tcccgggatg ccgcgcgccg
     4201 gagcgacgtt gccgagatcg aagaggagct cgatgcccgg gccgaggtga tgaccggcga
     4261 ggcgaatgcc gagatcgacg gcggtcgcga gatcgaacca gtgcggctcc tcggtgacga
     4321 gcgcgcgatc gagctcttcg acgcggcgcg cgagctgcgt cgcttcgaga tacgcggacg
     4381 cgaggtcttg ccgcagggtc tgcagcgagc gatatggata cggcgtcggt cccgtcgagg
     4441 tcgcgacgtg gcgctcgatg tgatcgacga cgaggccgag gcggtcacgc agtgcgcgca
     4501 acggctcggt taactgccgc gcgacctcga cggcgacgtc accaccgact tgcttcgcgg
     4561 tcacaggctc cccgtgagca ggaaccaggt ttcgttgatg ccctcgtcgg tcaggcctcg
     4621 tgaataaccg atctcgaacg tgccgggcac gaagtagccg aagaacgcgt cgagccgcag
     4681 tgccgcgccg accgacgtgc ggagatcgcg attggcatcg aacgtcgtgt cgaacgcgac
     4741 accggtatcg ctgagcagcg cgaaatgcag ccggcggaaa tacaccggca gcgtctgcag
     4801 accgcgctcg acgttccata gctcctggcg atattcgaga ttgagcaggt gaaactgatt
     4861 gccggcgacg gtgcgcgcgg gatagccgcg caagtagccc gacgacgcgc tgcggagcga
     4921 gttgacgatc gaattcgcga cgtcttgcgg cggcacgccg ccgaggccgt aggagccggc
     4981 gcgcggcagg tcaccgaccc ggaacgcacc gacgagccgc gccgacagca ccggcgtgtc
     5041 gccccagagt ttctggaagc gatcggcagc gtaggagaac gtgacgttgc gatacttggc
     5101 gccgatcgcc ggatgatcga agcgaatcgc gacgctgccg tcgaagccgc tctgcgagcc
     5161 caggccgaac gtcgtgccgc ggatcgtcga gaacgcgacg cgcgagccga tgccggcctg
     5221 cacgtaatcc gtcggcggat gcatcggcgt gtgtgcgttc ggatcgagca ccatgtccgg
     5281 cgctttgacg aggcggaacc aatcgacgtc gtagtcgaac gcggcggtcc agctcgagct
     5341 cggccgcgat tcgaacggaa cgccgacgga gatcgtcgcg ctccagtcct cttccttgaa
     5401 gcgtttgggg acgccgtcga cgcgccagcc gccgcgctcg agcaacgtcc gcgacaccgc
     5461 gccgcgaatc gcgggcaaca ggccaaagta tccgtacgac gcgccgatgt tcgcgtcgcc
     5521 ggtcgcgtag tcgagaccga gcgccatcga gtagccgtgc agaccgaccg catcggagcc
     5581 gttcgtcgcg atatttgcgc tctgcgatgt cgtgtcgacc tgcagcgtcc acacctgcgg
     5641 cgcgagcgac tccatcgcgc gatacggccg cgcctgggtg accacggctt catcgtcggc
     5701 gatgactacc ggatcgggcc gatcgtcgac gaagtcacgc gccggcaacc acctgctgcg
     5761 atcgacgggc agctcgtaga ggtcgtagcc gcccttcggc accgacgcga tgaacgcgat
     5821 ccgcttgcca tcgggcgatg ccgccggcga gaacgcgccg ccgagcacgt tcgtgagctg
     5881 ccaggtcgta cggtcgtgca gatcgaacgc gtagatgttc gagatgccgg tgcgatcgct
     5941 tgcgaagtag agggtcgagc catcggccgc ccactgcggc tggatgtcga tcgcgcgatc
     6001 gttcgtgatc tcgtcgatct tgccggtcgc gacgtcgacg atcaggatgt cgcgatagcc
     6061 gggacgccgc cacgccgaga acgcgatgcg cgtgccgtcg ggcgaccacg cgggttgata
     6121 cgcctggtcg taacgtttgc cgcgccatac ggtcgtcgcc ggcgcatcgg gcgcgaggtc
     6181 catgaccgcg agcgagctct ccgcgtgctc gttctgcgag tacgcgacgc ggcggccatc
     6241 gggcgatacc actggatcac gcgctcggcg gccggtcgtc agccgcaccg tctgccgggt
     6301 gcgcgcgtcc cagcgaaaaa tatcctcgta gccgtacacc gcgcggtact ggcggccttg
     6361 ctcgtaaacg agcgagccat cgggcaacag gtcgtactcg cccatcgcgt cgatctggac
     6421 gacgtcgtgc gccgtcgtga catcgccgcc gaccggcatt gcgcgcacct tcggcaggct
     6481 gtagccggtg tactcgagcc agtagagctc tttgccgtcg gcggagtagc gcgggatcaa
     6541 gttcgactcg ccgctgtgcg tgagtgcgcg accggtctgc atgccgcggc gttcggcagc
     6601 catttcttgc tggctgtact tgtcgcgcag ataggccttc c
//
LOCUS       DASUFX010000402         5267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1452875, whole genome shotgun sequence.
ACCESSION   DASUFX010000402 DASUFX010000000
VERSION     DASUFX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452875"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..434
                     /locus_tag="VFV99_16060"
     CDS             51..434
                     /locus_tag="VFV99_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060883.1"
                     /translation="MVAQPDDRDIEPELIRELAAELEIELALEYAFDPDLEVDIEIID
                     GSLVIARDTMPEIRTAIVIEAGHRTPRQPMLDYKHCNANIFDEFEDSDDEWFISFDET
                     QPIEFPDEPTTRWRRLRSWARKRAA"
     gene            553..1038
                     /locus_tag="VFV99_16065"
     CDS             553..1038
                     /locus_tag="VFV99_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060884.1"
                     /translation="MLIAILPLIVMTTGAFDAEIAHADAARTAERSPQVKLADLLGSA
                     DSIDSISGRGNTFRFVITRGDDHLELIATTNHRDVVSVVERERDFGGAGIIELANLSW
                     LIDTMQATTAVTRLEVDDDGAVTMTTDEGMRYMAIPGRGSGGNAAVEGRWAGEWNRDE
                     G"
     gene            1286..1822
                     /locus_tag="VFV99_16070"
     CDS             1286..1822
                     /locus_tag="VFV99_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060885.1"
                     /translation="MKASKFIVLVGGILGILAFFLPLVSVERNGAKASVSAYQVIKGL
                     DQVEVAVDQGAARGEYAVETTASAKKDIGAMKGIVMAIFAPALLLSLIGAAGVARKRF
                     GRVAGTFSFLFGIVGLGIAALLKSAAEGDSGIGLTLLLVTGVAGLVGGLAALIKPERL
                     ATGQPALAAVGTSTRIAA"
     gene            1873..2754
                     /locus_tag="VFV99_16075"
     CDS             1873..2754
                     /locus_tag="VFV99_16075"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5060886.1"
                     /translation="MKFDPRTDPDVPKGMSVELLADLHLLTRDGHLNADARRKLKQIR
                     HLVGLLRPGLDDALARHTDPLVVDCGAGKSYLGAMLYELVLGPAGRGSLMAIEARPEL
                     SEQAAKRAARFGQERFRVATGAIGSFITAPSASDAAQEPNAQRPNVVTALHACDTATD
                     DALVLAIAQAADHVAVVPCCQAEVARQLEAAKPSDPVDAALFEHPLHRREFGSHLTNV
                     IRALVLAAHGYKVTVTELVGWEHSAKNELILGKRVQRFDKHARATLRALLDRFHVEPA
                     VIRELASRGLDPRTEPA"
     gene            2793..3944
                     /locus_tag="VFV99_16080"
     CDS             2793..3944
                     /locus_tag="VFV99_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060887.1"
                     /translation="MRWALMLAWLCGCGRIAIDPIADDGAMPDAPPTGPGVFAVAAHV
                     NSSCMILDGALSCWGANANGQLATGDTMDRFARTPIGSERDWIAIAPGETHICGLRAG
                     GDLYCWGGNDNGELGQGDMMERHVPTRVPLARPVVAIDAWSHTCAILDNGELWCWGQN
                     AEGELGQNDSNGAAPIPSPVQVSPGMTWSRVGLGQGHTLAYGAAGLYGTGRNVDSELG
                     LGPTGSPQLRVMTAIDTAAWTTINGAQNSSCGRRADQTIACWGANDFGPLGTGDKLNR
                     DVPTTVGTTEMWRTVDIDTFHSCALAMTNQIYCTGRNVEGQLGTGDFMDRMDWTLSST
                     FSDWILLSVGRFHTCGMRADHSVWCVGQNTNGQLGVTGTQNRANWLQVL"
     gene            complement(3961..4413)
                     /locus_tag="VFV99_16085"
     CDS             complement(3961..4413)
                     /locus_tag="VFV99_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEX5060888.1"
                     /translation="MGRMWIPLTVHAKTRRTFGALIDTGASVTVLSNAYAEMIGIDWK
                     AEDKLEIGGFRYPVCYRRVDMHIPRTDCFVENLRVAVIATPANDIPGAIIGADFLQRP
                     GAVLDVRKGRHAVGGDLEGRDANPPELVRAKPLRVKRAKPARRRNGRS"
     gene            complement(4432..4854)
                     /locus_tag="VFV99_16090"
     CDS             complement(4432..4854)
                     /locus_tag="VFV99_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060889.1"
                     /translation="MNFIELPHSLRSLATWIGSTAFATALFWSCFGTCVAGCIDQPLP
                     DVEPQARVVASWDPLQCGDPHRVVVELEDDDGAKLSHSVPCEVGGMSIDIRHWGVYRG
                     RIYAWSLGTGNAAEMRSVLYVRLDVDAPVIFWPVDTPR"
     gene            complement(4971..>5267)
                     /locus_tag="VFV99_16095"
     CDS             complement(4971..>5267)
                     /locus_tag="VFV99_16095"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEX5060890.1"
                     /translation="RMTQQRVQYVLVDQAARALDHGLGEQLLAQHERCDAWRDALRDM
                     PLHGGLVVADVDGPELFAVASELFFERPMTLMRIDPQLFFELVSIYKIDTRSLA"
BASE COUNT          937 a         1778 c         1717 g          835 t
ORIGIN      
        1 cgagcagaag ttgatcgcgc tcggcaaacc gacgacgttc accgcccgcg atggtggcgc
       61 aacccgatga tcgcgatatc gagcccgagc tgatccgcga gctcgcggca gagctcgaaa
      121 tcgagctcgc tctcgagtac gcgttcgacc cggatctcga ggtcgacatc gagatcatcg
      181 atggctcgct cgtgatcgcg cgcgacacca tgcctgaaat ccgcacagcc atcgtgatcg
      241 aggcaggtca ccgcactccg cgccagccga tgctcgacta caagcattgc aacgcgaaca
      301 tcttcgacga attcgaagac tcggacgacg agtggttcat ctcgttcgac gagacgcagc
      361 cgatcgagtt tcccgacgag ccgaccacgc ggtggcgccg cttacgctcg tgggcccgca
      421 agcgcgccgc gtaatgcgga cttaggaaac gggttcagac cccatttctg tgtctccaca
      481 gccacgcgga cggttaacta ggccggccgc cggcacatct gggctggccc ggcggtcgca
      541 ataccgacgg gcatgctgat cgccatcctg ccgttgatcg tcatgaccac cggtgcgttc
      601 gacgccgaga tcgcgcacgc cgatgccgcg cgtacggcgg agcgtagccc gcaggtgaag
      661 ctcgccgacc tgctcggtag cgcagactcg atcgacagca tcagcggtcg cggcaacacg
      721 ttcaggtttg tgatcacgcg tggcgacgac cacctcgagc tcatcgcgac gacgaaccat
      781 cgcgacgttg tcagtgtcgt cgagcgcgag cgcgacttcg gcggcgcagg tatcatcgag
      841 ctcgcaaacc tgtcgtggct catcgacacg atgcaggcga ccaccgccgt cacgcggctc
      901 gaggtcgacg acgacggcgc agtgaccatg accaccgacg aaggcatgcg ctacatggcc
      961 atcccgggcc gcggctcggg tggtaacgcc gccgtcgaag gccggtgggc cggtgagtgg
     1021 aatcgcgacg agggctgaat cgaaagcgcg atccgttgtg agcgcgctcg ccgccgctgg
     1081 tcgcacgaaa tcgtcgacga tttttcatcg tcatcaccga gagctacaac ggacccagcc
     1141 gatctcggcg atcgggtccg actagccccg acaggcgccg ccaagtgcgc gactccaaat
     1201 cgcggtcgct tggccttcga gctgcacata ggccggcctc gtcaaacaga ggcacttggt
     1261 gcctcgcaac tcggaggcat cggtcatgaa ggcaagcaag ttcatcgttc tcgtcggcgg
     1321 catcctcggc atcctcgcgt tcttcctccc gctcgtctcg gtcgagcgga acggtgcgaa
     1381 ggcgtccgtc agcgcgtatc aagttatcaa gggactcgac caagtcgaag tcgcggtcga
     1441 tcagggcgcc gcacgcggcg agtacgcggt cgagaccacc gcgtccgcaa agaaggacat
     1501 cggcgcgatg aagggcatcg tgatggcgat cttcgcgcct gccctgctgc tgtcgctgat
     1561 cggtgcagcg ggcgtcgcgc gcaagcgctt cggtcgcgtg gcaggcacgt tctcgttcct
     1621 gttcggcatc gtggggctcg gcatcgcggc gctactgaag agcgcggctg aaggtgacag
     1681 cggcatcggt ctgacgctgt tgctcgtgac cggtgtggcc gggctcgtcg gtggcctcgc
     1741 cgcgctgatc aaacccgagc ggctcgcgac cgggcaaccg gcgctcgcag cagtcggcac
     1801 cagcacgcgc atcgcggcct gaactgcggc ccgagcagca gcgcgatgcg cgattagcgc
     1861 tacagtgcgc gagtgaagtt cgacccgcgc accgatcccg acgttccgaa aggaatgtcg
     1921 gtcgagctgc ttgccgacct gcatctgctc acgcgtgatg gccacctcaa cgccgacgcg
     1981 cgtcgcaagc tgaaacagat ccggcatctc gttggtctgt tgcgccctgg actcgacgac
     2041 gcgctcgcgc ggcataccga tccgctcgtc gtcgactgcg gcgccgggaa gagctacctc
     2101 ggcgcgatgc tctacgagct ggtgctcggc cctgccggac gcggttcgct gatggctatc
     2161 gaagcgcggc ccgaattgtc ggagcaagcc gcgaagcgcg ccgcacgatt cggccaggag
     2221 cggtttcgcg tcgcgaccgg cgctatcggc tcgttcatca ccgcgccgag cgccagcgac
     2281 gccgcgcagg aaccgaacgc ccaacgcccg aacgtcgtca ccgcgctaca tgcctgcgat
     2341 accgccaccg acgacgcgct cgtcctcgcg atcgcgcaag cggccgatca cgtcgcagtc
     2401 gtcccatgct gtcaggccga ggtcgcgcgc cagctcgaag cggcgaagcc gagcgatccg
     2461 gtcgatgccg cactgttcga gcatccgttg catcgccgcg agttcggctc gcacctgacg
     2521 aacgtgatcc gcgcgctcgt gctcgcagca cacggctaca aggtcaccgt caccgagctg
     2581 gtcggctggg agcactcggc gaagaacgag ctgatcctcg gcaagcgcgt gcagcgcttc
     2641 gacaagcatg cgcgcgccac gctgcgcgca ctgctcgacc gcttccacgt cgagccggcc
     2701 gtgatccgcg agctcgcgtc gcgtggtctg gatccacgta ctgaaccggc gtagcgcacc
     2761 gcgagcggcg tcccctgtac tatggatcgt gcatgcggtg ggcgctcatg ctcgcttggc
     2821 tgtgcggttg cggccggatc gcgatcgatc cgatcgccga cgacggtgcc atgccggacg
     2881 ctccgcccac cggccctggt gtgttcgcgg tcgcagcaca cgtgaacagc tcgtgcatga
     2941 tcctcgacgg cgcgctgtcg tgctggggcg caaacgcgaa cggtcagctg gcgaccggcg
     3001 acacgatgga tcgctttgca cggacgccga ttggcagcga gcgcgactgg atcgcgatcg
     3061 cgccgggtga gactcatatc tgcgggctgc gtgccggtgg cgacctctac tgttggggcg
     3121 gcaacgacaa cggcgagctc ggacaaggcg acatgatgga gcggcacgtt ccgacgcgcg
     3181 tgccgcttgc acggcccgtc gtcgccatcg atgcgtggtc acacacctgc gccatcctcg
     3241 acaacggcga gctgtggtgt tggggccaaa atgccgaggg cgagctcggg cagaacgact
     3301 cgaacggagc agctccgatc ccatcgcccg tgcaggtcag tcccggcatg acatggagcc
     3361 gcgtcggcct cggccagggt cacacgctcg cgtacggcgc agcgggcctc tacggtaccg
     3421 ggcgcaacgt cgacagcgag ctcgggctcg ggccaaccgg atccccgcag ttgcgcgtga
     3481 tgaccgcgat cgacaccgct gcatggacca ccatcaatgg cgcccagaac agcagctgcg
     3541 ggcggcgcgc cgatcagacg atcgcgtgct ggggcgcgaa cgacttcggt ccgctcggca
     3601 ccggcgataa actcaatcgc gacgtcccga ccacagtggg caccacagag atgtggcgta
     3661 ccgtcgacat cgacacgttt cacagctgcg cgctcgcgat gacgaaccag atctattgca
     3721 ctggtcgcaa cgtggagggc cagctcggca ccggcgactt catggaccgc atggactgga
     3781 cgctctccag cacgttcagc gactggatcc tgctctcggt cggccgcttt cacacctgcg
     3841 gcatgcgcgc cgatcactcg gtgtggtgtg tcggtcagaa cacgaacggc cagctcggcg
     3901 tcaccggcac ccagaatcga gcgaactggc tccaggtgtt gtgagcgaac cgcccgctat
     3961 tcagctgcgg ccgttgcggc gccgcgccgg cttcgcccgc ttgacccgca ggggcttcgc
     4021 acgaacgagc tccggtggat tcgcgtcacg gccctcgaga tcaccgccga ccgcgtggcg
     4081 gcccttccgg acgtcgagaa ccgcgcccgg ccgctgcagg aagtcagctc cgatgatcgc
     4141 gcccggaatg tcgtttgctg gtgttgcgat cactgcaacg cgcaggttct cgacgaagca
     4201 atcggtgcgt gggatgtgca tgtcgacgcg ccggtaacac accggatagc ggaagccgcc
     4261 gatttccagc ttgtcctcgg ccttccagtc gatgccgatc atctcggcgt acgcgttcga
     4321 caggaccgtc acgctagcgc ccgtgtcgat gagcgcgccg aacgtgcggc gcgttttcgc
     4381 gtgcacggtc agcggtatcc acatacggcc catcacatca ccagggtagc actagcgtgg
     4441 cgtgtcgacg ggccagaaga tgaccggcgc gtcgacgtcg agccgcacgt agagcaccga
     4501 tcgcatctcg gccgcattgc cggtgccgag cgaccacgca tagatgcgtc cgcgatagac
     4561 gccccagtgc cggatatcga tcgacatgcc accgacctcg cacggcaccg agtgcgacag
     4621 cttcgcgccg tcatcgtctt cgagctcgac cacgacgcga tgtggatcgc cgcactgcag
     4681 cggatcccac gacgcgacga cacgcgcctg cggctcgacg tccggcaacg gctgatcgat
     4741 gcagccggcg acgcacgtgc cgaagcacga ccagaacaag gcggtcgcaa acgcggtgga
     4801 accgatccac gtggcaagcg atcgcaacga atgaggaagc tcgatgaagt tcatgccgcg
     4861 tgcttcagca cggcgcgggc caacgcgaag cgcgcgaatt cactcgcggc gagtgtccgt
     4921 gtccgacggc gtgcggacag gccgtccatg cgccggacaa cgagacggcg ttacgcgagc
     4981 gagcgcgtgt cgatcttgta gatgctgacc agctcgaaga acagctgcgg atcgatccgc
     5041 atcagcgtca tcgggcgctc gaagaacagc tcgctcgcga ccgcaaacag ctccggccca
     5101 tcgacgtcgg cgacgacgag accgccatgc agcggcatgt cgcggagcgc gtcgcgccat
     5161 gcatcgcacc gctcgtgctg cgcgagcagc tgctcgccga ggccgtgatc gagcgcgcga
     5221 gccgcctgat cgacgagaac gtactgcacg cgctgctgcg tcatccg
//
LOCUS       DASUFX010000403        19752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_759823,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000403 DASUFX010000000
VERSION     DASUFX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19752
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759823"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFV99_16100"
     CDS             complement(<1..1155)
                     /locus_tag="VFV99_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060891.1"
                     /translation="MQAVEPGWATRFYGVTVNKEVAAAARDHLTDRTITDLVDAHLAI
                     NQAYFGGIDKTLSGFVILDDEGDDYTLIDLRDSGQLWWQDHETRELSLRFNNLSDMAA
                     NRTAKEAKHRRRVTTPQLCERYQWLVWFYAQPLLQDGTPTQTTDHLVRNSLGRFRQAF
                     PTRDALYTTFAAEMKELADDPHLAIYWLLHTTALADHERRQRVLIAAGTSDNPLLRAF
                     IARLGQLPLAGDLPIVPDFRARRALAQSYGAFELTAEQIPAACLRALEIAPATESLGH
                     GLQIVWGLDRGLLDDETVMATLARIPEQTTGTSLVAAVLEKRRKAGSSSHADVLVRLL
                     ATSTDPWWTQLEALWHVHELAYDGPALVTATRRVLVHDRYHRRALQMAMRA"
     gene            1207..2049
                     /locus_tag="VFV99_16105"
     CDS             1207..2049
                     /locus_tag="VFV99_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060892.1"
                     /translation="MGLAPEDWEEHTEIVPRVVLARRRRAVTFLAFAAFAFSVGAASF
                     VIPKKRRVEQATKDAITADAQKLAVNLHAAFNQTQDRAEGFAGTPVLEAGILTDAATV
                     ADIVKTEYRLKTRPGETLELFQLKGETITPLVRVPADAAPIAYVKGKRTRIENRKGLA
                     VVVSAEVAPYNSTTDLSGQLAVAAAVDLSQLPLSAHSVEANLVGAGPPIPLVAKRGTG
                     DSLTVPVPLDAKWELGQLSLHAVPVMSPYLAGWVLPLRYGALGLGIAFLCTFLVAFRG
                     ALRR"
     gene            2078..5113
                     /locus_tag="VFV99_16110"
     CDS             2078..5113
                     /locus_tag="VFV99_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060893.1"
                     /translation="MRVIEQARLWFREGNSDKVYEIDLVEVAAGQHVVNFRYGRRGGP
                     LKDGTKTPLPLSLDKARGVYKKLVDEKVAGGYQTGPVTAAPAPAPVASGSPYRSPPVV
                     HTPSVAQQLIGKLRMGARSPSPLGPVMWRVADLDLTEAEPVVLELLASQFVPTELEPN
                     AWRHLLVAALARMGSAAAVTPLANLITDARTPRHLVDVARIAIARIDPVRSKEIALQR
                     LPQNLSALYTRGDGPALARAVEEALTSDPMKVRDPIVALYAINDAVARPAVLAVARVA
                     RLSNVEAAIVRTLFRLAEIRRDGELYALIARRIDAHRGHTRPFSPLTRNYLRRRVARY
                     LRRLGHVESADYVPMAAAILLGYEDDDTPDPKRGVFNHTYDRYAAYHAFNQILFTHSS
                     RYEKSHHELSVWRVKGRYRPGDPPPPEREEAFPALWDRAPATLWELILKSGATPVLEF
                     ATRALRANHAFTQALRDDQLALVMSSGHLIAQRFAFDIARQRSLNVTLARGALSSDLP
                     EAHNWVVTWIDANPEATVGDPDMLAMIVTGKTQAVREATLRLLRARPIADDIARSASV
                     RAIAILLGLGDTPANAERASGAVATLLRSFEAQLREVSAEVLRDMLKHPLAALGELAG
                     ELVLRHAHRDTLPVDLIETMLASKFATVRTLGGRVLAATPPEVAKDDLEALVMFATSG
                     NAELREATRTLLGEVAKRYPDVGRTLADRLIDALLKTQPPGAPAHIVTLLKAELAPVL
                     PQKPAQTILRLIGALSPHARDAGGLLLHQLAPDDIGLDDIARLASHENLSVRQGAWAL
                     ARASVSRYRIAPVALAKLLDSDWEDTRQFAIGFIRDDIGGDKLSADAIITICDSIRPE
                     VQDLGKSLLHQQFREADAPRYLERLAEHPSTNLQLLVSTLLDRAFDFGTDIQKLQILV
                     PYFATVLSQVNRGRVAKERVLALLRREAAKSHEAAAVLAPLLDRQSATAAITQKHPII
                     ATMVDVHHLYPDVPLPISIIVPAPPAKPGALHSRPKGAS"
     gene            5113..7647
                     /locus_tag="VFV99_16115"
     CDS             5113..7647
                     /locus_tag="VFV99_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060894.1"
                     /translation="MLFEHRYSGSSAVRQDQDATGMSFAPDTLREPTYFRGKLTRQIE
                     FREAISALHDVVTSDLRFIPKDRTAYLQWREQQSYIDLAAVSSRQVQLAADITKAQQE
                     LSALEHAASHRRSGFYKARQKYFDYLYNRDREMWFKLDPVITVHPDQVFFECFSKDES
                     SYGRLAASYNVFEELGERANGTTNIDYSEKLYGEFQKIRSYKTTTLDVDPGGFNVKTS
                     MEEQYREVKIDLPDSWVRGFLQVSSAATLPGNVVELHPMDVYNLCHVLRRNKELFGPR
                     SLRFKLTPGAPVEIVVEPWNHVVKCPRSRQTDGTATEIRVWGRRRLFILERLLPRAKR
                     VRVFLLGTGLPTFWVVDLGELSFTLGLSGWTQNNWSEAGNFDLMAAREEVDNITQQRV
                     FNELGKVWLATPEELAKTTGLATPVVASALAGWVQAGRAIFDLDRGVYRKRELTREPL
                     PMDKLRFANEREESAAQMLHRAKIAVDKADTSDGGLALAGRIHDYSKLYGVALTFDAD
                     RRLVQGECSCDFFIRNRLHRGPCEHMLALRAAHRRGVNDVITFTPQQTTSRGAVPQQS
                     TQQLRQSFQDAYRRSVARAAELRQQGRAQDAIAELERVLRIAPPNSDEEGRLNEVIAE
                     ARFDAGDHQAAQQAADRALTRFPTSILALRIKRDAFVELDDIEQAIPVARALAHIDNK
                     PERWDELCTLCDSIADHRAMFQFAEEGLRLHKGHPKLTQHRERARGNLPPGSVPPAPA
                     APPTPDAAKPSWWRRAVGAITGKPPTTPPPTLDGRLDRIMGELAQGSTIRDREAVLQL
                     LRVAHAAADRDDARVFALAAALKDTPFISNLPDDQELLALLRRALS"
     gene            7904..9328
                     /locus_tag="VFV99_16120"
     CDS             7904..9328
                     /locus_tag="VFV99_16120"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX5060895.1"
                     /translation="MADNLSPPKNRKELYERIARGGKDEVIIEEMVRLGFWPNTSPPP
                     SDPANEVRRMGELRARLTQLRQQAQGLRNLAQLEKDLKKQRLAESKKKRELNKQKLLG
                     ERAASKAETQQKKTRELTYLGPAVSVGLGPVADRRVSKPELLAKQKLPLANTAAELAA
                     QMKLPLGRLRYLAFAREVSTTSHYHRFTIPKRTGGERLISAPHKKLKAAQHWILENVL
                     EPLALAEHAHGFVPGRSTVSNALPHVGAAVVVNVDLRDFFPTVTYRRVKGLYRKLGYS
                     EEVATVLALICSEPDVTETELDGTRYYVARGPRRLPQGAPTSPAITNVLCRRLDRRIA
                     GWARKHGFMYTRYADDLTVSSKDRAAPVGKMLAFLRHITTAEGFAIHPDKVRVVRRGR
                     RQEVTGVVVNEKPGVPRDELRRFRALLYQIEKDGPAGKKWGEGGTDVLTAALGFAAYV
                     AMVDKAKGEVLRAKVLALKAKYKS"
     gene            9411..10313
                     /locus_tag="VFV99_16125"
     CDS             9411..10313
                     /locus_tag="VFV99_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5060896.1"
                     /translation="MRNQLKTILLFGVLSALLIGIGGALRPNQLGLFIAIAAVMNIVS
                     YFWSDKIVLRIYRARPLSETDAPGLHAMVSELAARAQIPKPRLFIVPGAQPNAFATGR
                     NPKHGAVAVTEGLMQMLDHREVRGVLAHEMAHIKNRDVLISTAAAVIASAITTVANVL
                     TFGAMFGGRRSSSEGGGNPLVLLFTIIVAPIAAVLIQLAISRSRELAADQTGAQLDGD
                     PDGLINALVKLERAAKVVPPAHAEPATANLFIVNPFGAREAMAKWFSTHPPMAERISR
                     LEELRDDSGELRARRFPRHAAHHV"
     gene            complement(10233..11006)
                     /locus_tag="VFV99_16130"
     CDS             complement(10233..11006)
                     /locus_tag="VFV99_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5060897.1"
                     /translation="MDISLREHWFEHGGTRLFATESGDGPPLVLLHGGLANHLACWQF
                     AAPLATRYRVITPDLRGAGRSHFAAPLSWDALADDIAALLLRLGIARAAIGGISFGAG
                     AAVRVALRHPDVVSALLVLHPAYGGAALGLSAMQQQAMAAMHAAGSRVVAEGIEVLMP
                     LFDALPPEVRERAKRVVATYDPASVATTTAFMQSGAQPFARGDELAAIRARTLVVPGV
                     DPYHPPEVAAVFQSHVVRCVTREAASPELAGVIAEFLEA"
     gene            complement(11118..11753)
                     /locus_tag="VFV99_16135"
     CDS             complement(11118..11753)
                     /locus_tag="VFV99_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060898.1"
                     /translation="MRSNRTTCFSLICGLVASGFLVLGSAGCKQDASAQGYAEVPQDP
                     VGPINCFRIVDAAQLSGQSAAELCTSALTDAPGRCFVATQDQFHELSTQDSLNLCDRA
                     TTMTPVDCYGHLRAEGVLTEDQMIDYCRTYCSLGPPPPEASNPACLSQAIENTDLSLQ
                     SAAELCLRSRTTYPVACFNEGQTLHKIADSTLVTMCSEQRRCQYYNAPPVY"
     gene            complement(11790..13028)
                     /locus_tag="VFV99_16140"
     CDS             complement(11790..13028)
                     /locus_tag="VFV99_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060899.1"
                     /translation="MQETNEMQRLTFAIQGAMRAASNPGGSNEEWGPQVYGDGQQQNE
                     LRERVMEAVRDRVGQRLNERLREVVKERMRDIVRERLATELRTAMIEAQSERGFDFSR
                     LERRLTERLAQTVEMHVMDAVHQRVRETIRERLGESVREALQNSPFAEQSEKIMMKIR
                     DRVEPELKERLVEGIRDRLRETIRTRMQETLRERLGEAFQAARQGGLSDPERTIGTIR
                     DRLGDAIEQRVGDAIAERVRESVREKLEEVVRDRVANAVRSAISERFQGGGRQMGFGG
                     GGFGFGSLTMTTDGANMSDWLQEQIYDQLRTRFMDQVRENVSVALKEHIEDVLRERLG
                     TAIRASLVEQKLLGGFNPEHFADSVRGRIAYAIRERLTDAVREKLHELHRDRLRDAIR
                     SAIGEQMGGSEFQGFGTGMH"
     gene            complement(13219..13491)
                     /locus_tag="VFV99_16145"
     CDS             complement(13219..13491)
                     /locus_tag="VFV99_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060900.1"
                     /translation="MNEVTRSQLLKELAARGYRDDGSSPNRTIVIDYDELSDVELGEL
                     LEQTVARREKIFRAVDTLGKDAAMQSYDDAQRAIDAMKVVIRKLTD"
     gene            complement(13488..15305)
                     /locus_tag="VFV99_16150"
     CDS             complement(13488..15305)
                     /locus_tag="VFV99_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5060901.1"
                     /translation="MSSTAQHYRPNLRDIFFNLFEVLDIQSSVLGKAPFDSMDEDSAR
                     ASLEGFLEVMLATWAPGFVPGDREGATFDGKGNVKLPAAYHKALDAYYNGGWNKLELP
                     EHLGGYGAPPSVRWSAFELMAGANPAVCFYVLGNVMATIIDSLGTPSQKARYVKAMTD
                     HHWGATMVLTEPAAGSDVGAGTTKARQLDGDVWQLEGVKRFITNGDFDHPKNIVHMVL
                     ARPEGAGPGTKGLSMFIVPKYWVEADGAIGERNGAVVTKVEHKMGIRGSATCELTLGD
                     GAPCRGLLLGEVHDGIAQMFHVIEYARMGVGTKSMATLSTAYLNALDYTRVRKQGADL
                     TKASDKTAPRVEIIKHPDVRRMLMTQKAFAEGLRAVVLWTAHIQDQVALRGGHGNQEA
                     RPLDALNDFMLPLIKGYASEKVYELLAVSLQCFGGSGYCQDYPIEQYIRDQKIDSLYE
                     GTTHIQALDLFFRKIGRDRGATLQSLVGQITATIDSLPTELAVEKTALLAAVGDVQAI
                     MMTMMGKLGQSVYHVGMQGNRILFALAELVIGWRLAVNAKVALAKLPSAVGDDAAFYK
                     GKLAASRFYAKQVLPGIKLARTLVEQGDIELMELADDCW"
     gene            complement(15318..16121)
                     /locus_tag="VFV99_16155"
     CDS             complement(15318..16121)
                     /locus_tag="VFV99_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060902.1"
                     /translation="MSRLMCGLWFALAACGGGGGFPDAPESDSAGPTGTFSVTWSVVD
                     QNSQPISCDTVSAQAMTVLAHNLAIEGGSTQIFSCNTGMGMSQGLAPGDYEMNFELSS
                     GMFGALGMAAKQAPVAITANANLELAPVTFQVEAHGGIALKLATGKPNCDAVSAGGAG
                     ITAMTITLNHNSDLTCEPITLSISDGVTQPGGMYTINCTTPMTRVCIEENQTITAMGV
                     PSDSYTIRVKGYVGANLCYLNADSIQVPPLNKLLLGTLNLGQQTQTPGC"
     gene            complement(16152..16658)
                     /locus_tag="VFV99_16160"
     CDS             complement(16152..16658)
                     /locus_tag="VFV99_16160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5060903.1"
                     /translation="MQPDLAISVRTATAFDAPALAALVNHAYAIESFFVDGDRTNAGE
                     VERMLERGTFVVLEHAGGLAAAVYVERGYFGMLSVLPEMQGHGLGTRLVRIAEAMCES
                     AGCLEVNLRIINLREELGRWYRSLGYNEVGTTPYEHRPVKQPCHFVEMRKSLRPVTAI
                     TGTGAVAA"
     gene            complement(16791..16916)
                     /locus_tag="VFV99_16165"
     CDS             complement(16791..16916)
                     /locus_tag="VFV99_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060904.1"
                     /translation="MVKAVSDVTVGTVIVLFHVALADRNRKATRDQRAKQQHLTA"
     gene            17004..17180
                     /locus_tag="VFV99_16170"
     CDS             17004..17180
                     /locus_tag="VFV99_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060905.1"
                     /translation="MIEEKKSVTQLARYRESEEVFRVLDRVLISAICVAITVERDACA
                     HSNVTSRNNGCSSK"
     gene            17218..18183
                     /locus_tag="VFV99_16175"
     CDS             17218..18183
                     /locus_tag="VFV99_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060906.1"
                     /translation="MTSRVNAVHIVASVDGESANDSPAYARANQTVKLFAVIRAGDKF
                     YSDAPSPSLGGKRITVEPIARGPVIELAWSRIEPTTANLSNTASGTFRFEPIDYRATE
                     IADGRARTWIHVDVRPTLTTDHGNGVGTMRYQLAVTQGDHTLTTPGPEARRGRGSGGL
                     TDGVMRVSIRRDDSYLGFLTEMYGQPYVWASAGLSDTSHQSERLEGSDCADLMVYGAR
                     RMGKHVAYTWTGGLPKVTKLIAAGTRDEHGVYRDANGKPVPYTQEGDLVLFPRHVGAL
                     AADKGEMGVLDDQDLMLHTLFDSPKEQPIKDSGYAQYPVEIRRFR"
     gene            complement(18180..19346)
                     /locus_tag="VFV99_16180"
     CDS             complement(18180..19346)
                     /locus_tag="VFV99_16180"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX5060907.1"
                     /translation="MPQIAGFRGALWDGSKVQLDSVVASPVTGVKERLAAGELVRDPS
                     RALYRYHQTFTLGGRSLTRQTVVCALRLEPWTEGTIRPHEQTDPTARDAATAAINAAG
                     AHTEAVFAGYRDAAREVDRLFRGVESGRPTVEVTTPDGTIHRLWRVANAEVIGKVRTQ
                     FAPKKLHVLDGHARYEGMLAYAHKLGAENQLQYSSTKYGLATLVNLEDPALVVAARHR
                     LVRNDGIKRDNVLESAKKYFIIEKIAGAARDVDKVQSALGETVAHQPAFVAIFAGDGD
                     AWKLTLKPDISPVTEGVSVHRAIQKLDPVVVEHMFLAKVLQTAASTTQLDAAGVVDAV
                     ANGAAVGMVMRPLTLEQVLHADELGALLPFGSTAFHPPLANLIAFVIDADEDLV"
     gene            complement(19361..>19752)
                     /locus_tag="VFV99_16185"
     CDS             complement(19361..>19752)
                     /locus_tag="VFV99_16185"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX5060908.1"
                     /translation="DPETQPLMARMIGDLGAVLAGAARGEIPVGKLAWDARVAVCVVV
                     AAAGYPGSVRTGDVIGGLDSVSEDVVVFHAGTARKDGKLVTAGGRVLGVTALGVSVQQ
                     ARERAYAVVDAVQLEGKQSRGDIGARR"
BASE COUNT         3373 a         6843 c         6282 g         3254 t
ORIGIN      
        1 cgcacgcatg gccatctgca gcgcgcggcg gtgatagcga tcgtgaacga gcacgcgccg
       61 ggtcgcggtg acgagcgcag gtccgtcgta cgcgagctcg tgcacgtgcc acagcgcttc
      121 gagttgtgtc caccacggat cggtgctcgt cgcgagcagc cgcacgagca cgtcggcgtg
      181 cgagctcgag ccagccttgc gccgcttttc gagcacggcc gcgacgagcg aggtgccagt
      241 cgtctgctcg ggaatgcgcg cgagcgtcgc catcaccgtt tcgtcgtcga gcaatccgcg
      301 atcgagcccc cacacgatct ggaggccatg accaagtgat tccgtcgccg gcgcgatctc
      361 gagtgcgcgc aaacacgccg caggaatctg ctcggccgtg agctcgaacg caccgtagct
      421 ctgtgcgagc gcacgccgcg cacgaaagtc tgggacgatc ggcaggtcac cggcgagcgg
      481 caactggccg agccgcgcga tgaacgcgcg gagcagcgga ttgtccgacg tgccggccgc
      541 gatcaacacg cgctggcgac gttcgtgatc ggcgagcgca gtcgtatgga gaagccagta
      601 gatcgcgagg tgcggatcgt cggcaagctc cttcatctcc gcggcgaatg tcgtgtagag
      661 cgcgtcgcgg gtcgggaacg cctggcgaaa ccggccgagg ctgttgcgta cgagatgatc
      721 cgtagtctgg gttggcgtgc cgtcctggag gagcggttgc gcgtagaacc agacaagcca
      781 ctgatagcgc tcgcacaact gaggcgtggt cacgcggcgg cgatgcttgg cctccttggc
      841 cgtgcggttt gcggccatat cggagaggtt attgaagcgc agcgagagct cgcgcgtttc
      901 gtggtcctgc caccagagtt gcccgctgtc gcgtagatcg atcagcgtgt aatcgtcgcc
      961 ttcgtcgtcg agaatcacga atcccgacag cgtcttgtcg atgccgccga aatacgcctg
     1021 attgatcgcg aggtgcgcgt cgacgaggtc cgtgatcgtg cgatcggtca ggtggtcgcg
     1081 cgcagcggct gccacctctt tgttcaccgt caccccgtag aagcgcgttg cccacccggg
     1141 ctcgaccgct tgcatcggct gacaacgtta ccccaagccg acggcgaggg agtagtttca
     1201 ctagtgatgg gtttggcgcc ggaggactgg gaagaacata ccgagatcgt cccgcgggtc
     1261 gtactcgcgc ggcgacggcg cgcggtcacg ttcctcgcgt tcgccgcgtt cgcgttcagt
     1321 gtgggcgcag cgtcgttcgt gatcccgaag aagcgccgcg tcgagcaagc gacgaaagac
     1381 gcgatcaccg ccgacgccca gaagctcgcg gtcaacctac atgccgcgtt caaccagacg
     1441 caagatcgcg ccgaggggtt tgccggcacg ccggtgctcg aagccggcat cctcacggac
     1501 gccgcgacgg tcgccgacat cgtcaagacc gaatatcggc tgaagacccg gccaggcgag
     1561 acgctcgagc tgttccagct caagggcgag accatcaccc cgctcgtgcg cgtgccggcg
     1621 gatgcggcgc cgatcgcata cgtcaaaggc aagcggacgc ggatcgagaa ccgcaaaggt
     1681 ctcgccgtcg tcgtcagcgc cgaggttgct ccgtacaaca gcacgacgga cctctcgggc
     1741 cagctcgccg tcgcagcagc ggtcgaccta tcgcaactcc cgctctcggc gcacagcgtc
     1801 gaagccaacc tggtcggcgc aggaccgccg attccgctcg tcgccaagcg tggcaccggt
     1861 gattcactga cggtcccggt tcccctcgac gcaaagtggg agctcggcca gctgtcgcta
     1921 catgccgtgc cggtgatgtc gccgtacctg gcaggctggg tgctcccgct tcgctacggc
     1981 gcactcggtc tgggcatcgc gttcttgtgc acgttcctcg tcgctttccg cggcgcattg
     2041 cgacggtagc cagggcggct ttgctaggct cgccgtcgtg cgggtgatcg aacaggcacg
     2101 cctgtggttt cgcgaaggca attccgacaa ggtgtacgag atcgatctcg tcgaagtcgc
     2161 ggccggccag cacgtcgtca acttccgcta cggacgccgc ggcggcccgc tcaaggacgg
     2221 cacgaagacg ccgctgccac tctcgctcga taaagcgcgc ggcgtttaca aaaagctcgt
     2281 cgacgagaag gtcgccggcg ggtaccagac cggcccggtc accgctgcac ctgcaccggc
     2341 gccggtcgcg agcggctcgc cgtatcggtc accaccggtc gtccacacgc cgagcgtcgc
     2401 ccagcagttg atcggcaagc tccgcatggg cgcgcgcagc ccgtcgccgc tcgggccggt
     2461 gatgtggcgc gtcgccgacc tcgatctcac cgaagccgag ccggtcgtgc tcgagctgct
     2521 cgcgtcgcaa ttcgtgccga cggagctcga gccgaatgcc tggcgacact tgctcgtcgc
     2581 cgcgctcgcg cgcatgggct cggcggccgc cgtcaccccg ctcgcgaatc tgatcaccga
     2641 cgcgcgcacg ccgcgtcacc tcgtcgacgt ggcgcgcatc gcgatcgcgc gcatcgatcc
     2701 ggtacgttcg aaggaaatcg cgctgcagcg cctgccgcag aatctgtccg cgctctacac
     2761 gcgcggcgat ggtcccgcgc tcgcgcgcgc cgtcgaggaa gcgctgacca gcgacccgat
     2821 gaaggtgcgc gatccgatcg tcgcgctgta tgcgatcaac gacgcggtcg cgcgtccggc
     2881 cgtgctcgca gtcgcgcggg tcgcccggct gtcgaacgtc gaggccgcga tcgtgcgcac
     2941 gctattccgg ctcgccgaga ttcgccgcga cggcgagctc tacgcgctga tcgcccgccg
     3001 catcgacgcg caccgcggtc acacgcggcc gttctccccg ctcacgcgca attacctgcg
     3061 ccgccgtgtt gcccgctacc tgcgccggct cggccacgtc gagtcagcgg actacgtgcc
     3121 gatggccgcc gcgatcctgc tcggctacga agacgacgac acgcccgatc cgaagcgtgg
     3181 tgtgttcaac cacacgtacg atcgctacgc cgcgtatcac gcgttcaacc agatcctgtt
     3241 cacgcacagc tcgcgctacg agaagagcca tcacgagctg agcgtgtggc gcgtcaaggg
     3301 ccggtatcgc ccgggtgatc cgccgccgcc cgagcgcgag gaagcgttcc ccgcgctgtg
     3361 ggatcgcgca cccgcgacgc tgtgggagct catcctgaag agtggagcga cgccggtgct
     3421 cgagttcgca acccgcgcgt tgcgcgcgaa ccacgcgttc acgcaagcgc tgcgcgacga
     3481 ccagctcgcg ctcgtgatgt cgagtggaca cttgatcgcg cagcggttcg ccttcgacat
     3541 cgcacgccag cggtcgctca acgtcacgct cgcgcgcggc gcgctgtcga gcgacttgcc
     3601 cgaggcgcac aactgggtcg tcacctggat cgacgcgaac cccgaagcga cggtcggcga
     3661 tccggacatg ctcgcgatga tcgtgaccgg caagacccag gcggtacgcg aggcaacgct
     3721 gcgcctgctc cgtgcgcgcc cgatcgccga cgacatcgcg cgcagtgcaa gcgtacgcgc
     3781 gatcgcgatc ctgctcggcc tcggcgatac gccggcgaac gcggagcgcg catcgggtgc
     3841 cgtcgcgacg ctgctcaggt cattcgaggc gcagctgcgc gaagtcagcg ccgaggtgct
     3901 acgcgacatg ttgaagcacc cgcttgccgc gctcggcgag ctcgccggtg agctcgtgct
     3961 gcgccacgcg catcgcgaca cgctgccggt cgacctgatc gagacgatgc tcgcgtcgaa
     4021 gttcgcgacc gtgcgcacgc tcggcggccg cgtgctcgca gcgacgccgc ccgaggtcgc
     4081 gaaggacgat ctcgaggcac tcgtcatgtt cgcgacgagc ggcaacgcgg agctgcgtga
     4141 agcgacacgc acgctgctcg gcgaggtcgc gaagcggtac cccgacgtcg gccgcacgct
     4201 cgccgatcgc ctgatcgacg cgctgctcaa gacgcagccg cccggcgcgc ccgcgcatat
     4261 cgtcacgctg ctcaaggcag agctcgcgcc ggtgctgccg cagaaacctg cgcagacgat
     4321 cctgcgcctg atcggtgcgc tgtcgccgca cgcgcgcgat gccggcgggc tgctgttgca
     4381 tcagctcgcg cccgatgaca tcggcctcga cgacatcgcg cggctcgcga gccacgagaa
     4441 cctgtcggtc cgccagggtg cgtgggcact cgcccgcgcc tcggtgtcgc gctatcggat
     4501 cgcgccagtc gcgctcgcga agctgctcga ttccgactgg gaagacaccc gccagttcgc
     4561 gatcggcttc atccgcgacg acatcggcgg cgacaagctg tcggcggacg cgatcatcac
     4621 gatctgcgat tcgattcgcc ccgaagtcca ggacctcggc aaatcgctcc tgcaccagca
     4681 gttccgcgag gccgatgcgc cgcgctatct cgagcggctc gccgagcacc cgtcgacgaa
     4741 tctccagctg ctcgtgtcga cgctgctcga tcgggctttt gatttcggga ccgacatcca
     4801 gaagctgcag atcctggtgc cgtacttcgc gaccgtgctg tcgcaagtca atcgcggccg
     4861 cgtcgcgaag gagcgcgtgc tcgcgctgct ccgccgcgaa gccgcgaaga gccacgaggc
     4921 ggcagcggtg ctcgcgccgc tgctcgatcg ccagagcgcc accgccgcga tcacgcagaa
     4981 gcatccgatc atcgcgacga tggtcgacgt ccatcacctc tatcccgacg tgccgctgcc
     5041 gatcagcatc atcgtccccg cgcccccggc aaagccggga gcgctccatt cccggccgaa
     5101 gggagcctcg taatgctgtt cgagcatcgg tactcgggct cgtccgcggt acgccaggat
     5161 caagacgcga ccggcatgag cttcgcgccc gacacgctgc gcgaaccgac gtacttccgc
     5221 ggcaagctca cgcgtcagat cgagttccgc gaggcgatca gcgcgctgca cgacgtcgtc
     5281 accagtgacc tgcggttcat tccgaaggac cgcaccgcgt atctgcagtg gcgcgagcag
     5341 cagagttaca tcgatctcgc cgcagtaagc tcgcgccagg tccagctcgc cgccgacatc
     5401 acgaaggcac agcaagagct gtccgcgctc gagcacgcgg cgtcccatcg ccgcagcggc
     5461 ttctacaagg cgcgccagaa gtacttcgac tacctctaca accgcgaccg cgagatgtgg
     5521 ttcaagctcg atccggtcat cacggtccac ccggaccagg tgttcttcga gtgtttctcg
     5581 aaggacgagt cgagctacgg ccggctcgcc gcttcgtaca acgtgttcga agagctcggc
     5641 gagcgcgcga acggcacgac gaacatcgac tacagcgaga agctgtacgg cgagttccag
     5701 aagatccgca gctacaagac gacgacgctc gacgtcgatc cgggcggctt caacgtgaag
     5761 acgtcgatgg aggagcagta ccgcgaggtc aagatcgacc tgcccgactc gtgggtgcgc
     5821 ggcttcctcc aggtcagctc cgcggcgacg ctgcccggca acgtcgtcga gctgcatccg
     5881 atggatgtct acaacctctg ccacgtgttg cgccggaaca aggagctgtt cgggccgcgc
     5941 tcgctgcgct tcaagctcac gcccggcgcg ccggtcgaga tcgtcgtcga gccgtggaac
     6001 cacgtcgtca agtgtccgcg ttcgcggcag actgacggca ccgcgaccga gatccgcgtg
     6061 tggggtcgcc gccgtctgtt catcctcgag cggctactgc cgcgcgcgaa gcgcgtgcgc
     6121 gtgttcctgc tcggcaccgg tctccccacg ttctgggtcg tcgatctcgg cgagctgtcg
     6181 ttcacgctcg ggctctcggg ctggacgcag aacaactggt ccgaggccgg caacttcgat
     6241 ctgatggccg cgcgcgaaga ggtcgacaac atcacgcagc agcgagtgtt caacgagctc
     6301 ggcaaggtgt ggctcgccac gcccgaggag ctcgcgaaga ccaccgggct cgcgacgccc
     6361 gtcgtcgcgt ccgcgctcgc gggctgggtc caggccggcc gcgcgatctt cgatctcgat
     6421 cgcggcgtct atcgcaagcg cgagctgacc cgcgagccgc tgccgatgga caagctgcgc
     6481 ttcgcgaacg agcgcgagga atccgcggcg cagatgctcc accgcgcgaa gatcgccgtc
     6541 gacaaggccg acacttccga cggtgggctc gcgctcgcgg gccgcatcca cgactacagc
     6601 aagctgtacg gtgtcgcgct cacgttcgat gccgaccgcc gcctcgtgca gggcgagtgc
     6661 agctgcgact ttttcattcg caaccgtctg caccgcgggc cgtgcgagca catgctcgcg
     6721 ctacgcgccg cgcatcgccg tggcgtcaac gacgtcatca cgttcacgcc gcagcaaacg
     6781 acatcgcgcg gcgccgtgcc gcaacagtcg acgcagcagc tgcgccagag ctttcaggat
     6841 gcatatcgcc gctccgtggc gcgcgccgcc gagctccgcc agcaaggccg cgcgcaagac
     6901 gccatcgccg agctcgagcg cgtgctgcgc atcgcgccgc cgaacagcga cgaggaaggc
     6961 cggctcaacg aggtgatcgc cgaggcgcgc ttcgacgccg gcgaccacca ggccgcgcag
     7021 caggcggccg accgtgcgct cacgcgattc ccgacgagca tcctcgcgct tcgcatcaaa
     7081 cgcgacgcgt tcgtcgagct cgacgatatc gagcaggcga ttccggtcgc gcgtgcgctc
     7141 gcgcacatcg acaacaagcc ggagcgctgg gacgagctgt gcacgctgtg cgactcgatc
     7201 gccgaccacc gcgcgatgtt ccagttcgcc gaagaaggtc tgcgcctgca caaaggtcac
     7261 ccgaagctca cacagcatcg cgaacgcgcg cgcggcaact tgccgcccgg ctccgtgccg
     7321 ccagctcccg ccgcgccacc cactcccgac gctgcgaagc cgtcctggtg gcgccgcgcg
     7381 gtcggtgcca tcaccggcaa accgccgaca acgccgccgc ccacgctcga cggccgcctc
     7441 gaccgcatca tgggcgagct cgcccaaggc tcgacgattc gcgaccgcga agccgtgctg
     7501 cagctgctgc gcgtcgcaca cgccgcagcg gaccgcgatg acgcccgcgt gtttgcgctc
     7561 gcggccgcgc tcaaagacac cccgtttatt tccaacctgc cagacgatca ggagctgctc
     7621 gcgctcttgc ggcgggcgct ctcgtgatcg acagtagcta tcgcgtggcg ggtggcccta
     7681 gaccttgcaa tccgggcggt gcatcgccgt ccttgcgaac catccgcgca tacatcactg
     7741 gctcactcaa tactgtgtgt cggcaggtac taccaggaga ttccgagggg gttttcgcga
     7801 aaagcgaatg cacccggtag tacccgccgc cactcgagcc gagcgatcca gtcccaggcg
     7861 tagacgaagg tagccatccc gccacgcacc cttttctctc ttcatggctg acaacctctc
     7921 cccaccgaag aaccgcaaag agctctacga gcgcatcgct cgcggcggta aggacgaggt
     7981 catcatcgaa gagatggtac ggctcggctt ctggccgaac acgtcgccac cgccgagcga
     8041 tccggccaac gaagtgcgcc gcatgggcga gctacgcgct cgcctgaccc agctgcgcca
     8101 gcaagcgcag ggcctccgca acctcgcgca gctcgagaag gacctcaaga agcagcgact
     8161 cgccgaatcg aagaagaagc gcgagctcaa caagcagaag ctgctcggcg agcgcgcggc
     8221 cagcaaagcg gagacgcagc agaagaagac gcgcgagctg acgtacctcg gtcctgccgt
     8281 ctcggtcggc ctcggtcccg tcgctgatcg ccgcgtctcc aagcccgagt tgctcgcgaa
     8341 gcagaagctg ccactcgcga acaccgccgc cgagctcgcc gcgcagatga agctgccgct
     8401 cggtcgcctg cgctatctcg catttgcgcg cgaagtctcg accacctcgc actatcaccg
     8461 cttcacgatt cccaagcgca ccggcggcga gcgcctgatc agcgcgccgc acaagaagct
     8521 caaagcggcg cagcactgga tcctcgagaa cgtgctcgaa ccgctcgccc tagccgagca
     8581 cgcgcacggt ttcgtgcccg gccgctcgac ggtctcgaac gcgctgccgc acgtcggcgc
     8641 tgcggtcgtc gtcaacgtcg acctccgcga cttcttcccg acggtaacct accgccgcgt
     8701 caaaggcctc taccgcaagc tcggctatag cgaagaggtg gcgaccgtgc tcgcgctgat
     8761 ctgcagcgaa cccgacgtca ccgagaccga gctcgatggc actcgctact acgtggcacg
     8821 tggaccgcgc cgcctgccgc aaggtgcacc gacgtcgccc gcgatcacga acgtcctctg
     8881 ccgccgcctc gatcgccgca ttgccggctg ggcgcgcaag catggcttca tgtacacgcg
     8941 ctacgccgac gacctgacgg tctccagcaa ggaccgcgca gcgccggtcg gcaagatgct
     9001 cgcgttcctc cgccacatca ccacggccga aggcttcgcg atccatcccg acaaggtgcg
     9061 cgtcgtgcgc cgcggccgcc gtcaggaagt caccggcgtc gtcgtcaacg agaagccggg
     9121 tgtgccgcgc gacgagctgc gccggttccg cgccctgctc tatcagatcg agaaggacgg
     9181 tcccgccggc aagaagtggg gcgaaggcgg cacggacgtg ctgaccgccg cgctcggctt
     9241 cgccgcgtac gtcgcgatgg tcgacaaggc caagggcgaa gtgctgcgcg cgaaggtgct
     9301 cgcgctcaag gccaagtaca aatcgtaggt gccgcggcgg tgtgccgtag cgctctccgt
     9361 tcaggcaggt acctttacat cgccgggccg gaccacatgt tgatctcgac atgcggaacc
     9421 agctcaagac gattcttctc ttcggcgttc tgtccgcgct gctgatcgga atcggcggcg
     9481 cgctgaggcc caaccagctc ggcctgttca tcgcgatcgc cgcggtgatg aacatcgtca
     9541 gctatttctg gtccgacaag atcgtgctcc gcatctaccg agcgcgtccg ctctccgaaa
     9601 ccgacgcgcc cgggctgcat gcgatggtga gcgagcttgc agctcgggcg cagattccaa
     9661 agccgcgcct gttcatcgtg ccgggcgctc agccgaacgc gttcgcgacc ggccgcaacc
     9721 cgaagcacgg cgccgtcgcc gtgaccgaag gcttgatgca aatgctcgat caccgcgagg
     9781 tgcgtggcgt gctcgcgcac gagatggcgc acatcaagaa tcgcgacgtg ctgatctcga
     9841 cggcggcggc ggtcatcgca tccgcgatca cgaccgtggc gaacgtgctg acgtttggcg
     9901 cgatgttcgg cggccgccgc agcagcagcg agggcggcgg aaatccgctc gtcctgctgt
     9961 tcacgatcat cgtcgccccc atcgccgccg tgttgatcca gctcgcgatc tcgcggtcgc
    10021 gggagctcgc cgccgaccag accggcgcgc aactcgacgg cgaccccgac ggtctgatca
    10081 atgcgctcgt caagctcgag cgggcggcga aggtcgtgcc gccggcgcac gccgagccgg
    10141 cgactgcgaa cctcttcatc gtcaatccgt tcggcgcgcg cgaagccatg gcgaagtggt
    10201 tctcgaccca cccgccgatg gccgagcgga tctcacgcct cgaggaactc cgcgatgact
    10261 ccggcgagct ccgggctcgc cgcttcccgc gtcacgcagc gcaccacgtg tgattggaat
    10321 accgccgcga cctcgggcgg gtgatacgga tcgacacccg gcaccaccag tgtacgcgcg
    10381 cggatcgccg cgagctcgtc gccacgcgcg aacggctgcg cgcccgactg catgaacgcg
    10441 gtcgtcgtcg caacactcgc cggatcgtac gtcgccacga cgcgcttcgc gcgctcccgg
    10501 acctcgggcg gcagtgcgtc gaacagcggc atcaagacct cgatgccctc ggcgacgacc
    10561 cggctgcccg ccgcgtgcat cgcagccatc gcttgctgct gcatcgccga aaggccgagc
    10621 gcggcgccgc cgtacgccgg atgcagcacg agcagcgccg acacgacgtc gggatgccgc
    10681 agcgccacgc gcaccgccgc acctgcaccg aacgagatac cgccgatcgc cgcgcgcgca
    10741 atgccgaggc ggagcaagag cgccgcgatg tcatcggcga gcgcgtccca ggacagcggt
    10801 gccgcgaagt gcgagcgccc tgccccgcgc aggtcgggcg tgatgacgcg gtagcgcgtg
    10861 gcgagcgggg ccgcgaattg ccagcacgcc aggtgattcg cgaggccacc gtgcagcagc
    10921 acgagcggcg ggccgtcgcc actctcggtt gcaaacagtc gcgttccgcc gtgctcgaac
    10981 caatgctcgc gcagagagat atccatgatc tcgtccttcc gtggcgggcc gcgtgaagca
    11041 gccgccgaaa ctaccgaaaa tgaaaaagca ccagccgaca cgctggcgct ctccattgga
    11101 tatcacacgc gacgccgtca gtacactggc ggtgcgttgt agtactgaca gcgccgctgc
    11161 tccgagcaca tggtcacgag cgtgctgtcg gcgatcttgt gcagcgtttg cccctcgttg
    11221 aagcacgcga ccgggtacgt cgtccgtgag cgcagacaca gctcagcggc tgactgcagg
    11281 gacaggtccg tgttctcgat cgcttgcgac aggcacgctg gattactcgc ttccggcggc
    11341 ggcggaccga gcgaacaata cgtccggcag tagtcgatca tctgatcttc ggtgagcacg
    11401 ccttccgccc gcaggtgccc gtagcaatcg accggtgtca tggtcgtcgc gcggtcgcac
    11461 aggttcaggc tgtcctgcgt cgacagctcg tggaattgat cttgcgtggc gacgaaacag
    11521 cggcccggcg catcggtgag cgcggacgtg cacaactccg ccgcgctctg cccggatagc
    11581 tgtgccgcat cgacgattcg gaaacagttg atcggcccga ccgggtcttg cggcacctcg
    11641 gcataaccct gcgccgacgc gtcttgtttg catcccgccg aaccgagcac gagaaaaccg
    11701 gaagcgacga gcccgcagat cagcgagaaa cacgtcgtcc ggtttgatct catgcagctg
    11761 catcggtgca gcggccatgc ctcgagcggt cagtgcattc cagtaccgaa gccctggaac
    11821 tcggatccgc ccatttgttc gccgatcgca gaacgaatcg catcgcgcag gcgatcgcga
    11881 tgcagctcgt gcagtttctc gcgcaccgca tctgtcagcc gttcacggat cgcatacgcg
    11941 atgcgtccgc gcaccgagtc cgcgaagtgt tccgggttga agccgccgag cagcttctgc
    12001 tcgacgagcg acgcacgaat cgcggtgccg agccgctcgc gcaggacgtc ctcgatgtgc
    12061 tccttgagcg cgacgctgac gttctcgcgc acctgatcca tgaaccgcgt gcgcagttgg
    12121 tcgtagatct gctcctgcag ccagtcggac atgttcgcgc cgtcggtggt catcgtgagc
    12181 gagccgaaac caaagccgcc gccgccaaag cccatctgac gcccgccacc ctggaagcgc
    12241 tccgagatcg ccgaccgcac cgcgtttgcc acgcggtcac ggacgacctc ttcgagcttc
    12301 tcgcgcacgc tttcgcgaac gcgctcggcg atcgcgtcac ccacgcgctg ctcgatcgca
    12361 tcgccaagac gatcgcggat cgtgccgatc gtgcgctcgg ggtcggagag tccgccctgg
    12421 cgcgcggcct ggaacgcttc gccgaggcgc tcgcgcagcg tctcctgcat gcgcgtgcga
    12481 atcgtctcgc gtaggcgatc gcgaatccct tcgacgagcc gctccttgag ctcgggctcg
    12541 acgcgatcgc ggatcttcat catgatcttc tcgctctgct ccgcgaacgg actgttctgg
    12601 agcgcctcgc gaacgctttc gccgagtcgc tcgcgtatgg tctcgcgcac gcgctggtgc
    12661 accgcgtcca tcacgtgcat ctcgacggtc tgcgccagcc gttcggtcag ccgcctctcg
    12721 aggcgactga agtcgaagcc gcgctcgctc tgcgcttcga tcatcgccgt gcgcaactcg
    12781 gtcgcgagcc gctcgcggac gatgtcacgc atacgctcct taacgacttc gcgcagccgc
    12841 tcgttcaggc gttggccaac gcggtcgcgc acggcctcca tgacgcgctc gcgcagctcg
    12901 ttttgttgtt gtccgtcacc atagacctgc ggtccccact cctcattcga gccgcccgga
    12961 ttcgaagcgg cacgcatcgc cccctggatc gcgaacgtca gtcgctgcat ctcattcgtc
    13021 tcttgcatcg ccattgctct ctctcccctg aggttttcca aacgtccggg cgcccgctga
    13081 gcaaacgctg gaccagggga taagcggcac agcgcaagac tcggcagtcc gcgcagcgca
    13141 ggaaatcctc agccgtgcgc gccgatcgtg cgaaccagtc agcgggtcag acgctcccgt
    13201 acgtacgaat ccgtaacgtt agtccgtgag cttcctgatc acgactttca tcgcatcgat
    13261 cgcgcgctgt gcatcgtcgt agctctgcat cgcagcgtcc ttgccgagcg tgtcgacggc
    13321 gcgaaagatc ttttcgcgac gcgcaaccgt ctgctcgagc agctcaccga gctcgacgtc
    13381 gctgagctcg tcgtaatcga tcacgatcgt gcgattcggc gacgagccgt cgtcgcgata
    13441 gcctcgcgcg gcgagctcct tgagcagctg acttcgcgtg acttcgttca ccagcagtcg
    13501 tccgctagct ccatcagctc gatatcgccc tgctcgacaa gcgtgcgcgc gagcttgatg
    13561 ccaggcaaca cctgcttcgc gtagaaccgc gatgcggcga gcttgccctt gtagaacgcg
    13621 gcatcatcac cgaccgctga cgggagcttc gcgagcgcga ccttcgcgtt gaccgcgagg
    13681 cgccaaccga tcacgagctc ggcgagcgcg aacaagatgc gattgccctg catgccgacg
    13741 tggtagacgc tctggcccag cttccccatc atcgtcatca tgatggcctg cacatcgccg
    13801 acggcagcca acagcgcggt cttctcgacc gcgagctccg tcggtaggct gtcaatcgtg
    13861 gccgtgattt gcccgacgag tgactgcagc gtcgcaccgc ggtcgcgacc gatcttgcgg
    13921 aagaacagat cgagcgcctg gatgtgcgtc gtgccttcgt acagcgagtc gatcttctgg
    13981 tcgcggatgt actgctcgat cggataatcc tggcagtagc ccgagccgcc gaagcattgc
    14041 agcgacaccg cgagcagctc gtataccttc tccgacgcgt agcccttgat cagcggcagc
    14101 atgaagtcgt tgagcgcgtc gagcggcctc gcctcctgat tgccgtgacc gccgcgaagc
    14161 gcgacctggt cctggatgtg cgcggtccac agcacgaccg cgcgcaaacc ctcggcgaac
    14221 gccttctgcg tcatcagcat gcggcgcaca tccggatgct tgatgatctc gacgcgcggc
    14281 gcggtcttgt cggaggcctt cgtcaggtcc gcgccctgct tgcgcacgcg cgtgtagtcg
    14341 agcgcgttca agtacgcggt cgacagcgtc gccatcgact tcgtgccgac gcccatgcgc
    14401 gcgtactcga tgacgtgaaa catctgcgcg atgccgtcgt ggacctcgcc gagcaggagg
    14461 ccccgacacg gcgcgccatc gccaagcgtc agctcgcacg tcgccgagcc gcggataccc
    14521 atcttgtgct cgaccttcgt gacgaccgcg ccattgcgct cgccgatcgc gccatccgct
    14581 tcgacccagt acttcggcac gatgaacatc gacagaccct tcgtgccggg ccccgcgccc
    14641 tcgggccgcg cgagcaccat gtgcacgatg ttcttcggat gatcgaagtc gccgttcgtg
    14701 atgaaacgct tgacgccttc gagctgccag acgtcgccat cgagctggcg cgccttcgtg
    14761 gtgccggcgc caacgtcgct gcccgcggcc ggctcggtca gcaccatcgt cgcgccccag
    14821 tggtggtcgg tcatcgcctt gacgtagcga gccttctgcg acggcgtgcc tagcgagtcg
    14881 atgatggtcg ccatcacgtt gccgagcacg tagaagcaca cggccgggtt cgctcccgcc
    14941 atcaattcga acgccgacca ccgcacgctc ggcggtgcgc cgtaaccacc gagatgctcg
    15001 ggcagctcga gcttgttcca gccgccgttg taataggcgt cgagggcctt gtggtacgcg
    15061 gccgggagct tgacgttgcc cttgccgtcg aacgtcgcac cttcgcgatc gccgggcacg
    15121 aagccgggcg cccaggtcgc gagcatcacc tcgagaaacc cttcgagcga ggcgcgcgcc
    15181 gagtcctcgt ccatcgagtc gaacggcgcc ttgccgagca cgctcgattg gatgtcgagc
    15241 acctcgaaca ggttgaagaa gatgtcgcgg aggttcggcc gatagtgctg cgcggtagag
    15301 ctcacccgcg cactgtacta acaaccaggc gtctgcgtct gctggccgag gttcagggtg
    15361 cccagcaaca gtttattgag cggcggtacc tgaatcgaat ccgcgttcag gtagcagaga
    15421 ttggccccga cgtaaccctt cacacgaatc gtgtaggagt ccgacgggac ccccatcgcg
    15481 gtgattgtct gattctcctc gatgcagacg cgggtcatcg gtgtcgtgca gttgatcgtg
    15541 tacatgccac ccggctgggt cacgccgtcg gagatgctga gcgtgatcgg ctcgcaggtc
    15601 aggtcgctgt tgtggttgag cgtgatcgtc atcgcggtga tgccggcacc gcccgcgctc
    15661 accgcatcgc agttaggctt gccggtcgca agcttgagcg cgatgccgcc gtgcgcctcg
    15721 acctggaacg tgaccggtgc gagctcgaga ttcgcgttcg cggtgatcgc gaccggcgct
    15781 tgtttggcgg ccatcccgag cgcgccgaac atgcccgacg acagctcgaa gttcatctcg
    15841 tagtcgcccg gcgcgaggcc ctggctcatg cccatgccgg tattgcagct gaagatttgc
    15901 gtcgagccgc cctcgatcgc gaggttgtgc gcgagcaccg tcatcgcctg cgccgaaacg
    15961 gtgtcgcagg aaatcggctg gctgttctga tcgaccacgg accaggtgac ggagaacgtg
    16021 cccgtcgggc cggcactatc ggactcgggc gcatcgggaa agccgccgcc gccaccgcac
    16081 gcggcaagcg cgaaccacag accacacatc agtcgagaca tcgcctcgac tgtaacaccc
    16141 ggccgaattc ttcaggcagc gacggccccg gtacctgtga tggctgtgac ggggcgcaag
    16201 gatttgcgca tctcgacgaa gtgacagggt tgtttgaccg gccggtgctc gtacggcgtg
    16261 gtcccgacct cgttgtagcc gagcgaccgg taccagcgcc ccagctcctc gcgcaggttg
    16321 atgatccgca ggttgacctc gaggcagccc gccgactcgc acatcgcctc ggcgatgcgg
    16381 accaaccgag tgccgaggcc gtgcccctgc atctcgggta gcaccgagag catgccgaaa
    16441 tagccgcgct cgacatagac cgcggcggcc agtccacccg cgtgctcgag cacgacgaag
    16501 gtgccgcgct ccagcatgcg ctcgacctcg ccggcgttcg tgcggtcgcc atcgacgaag
    16561 aacgactcga tcgcgtatgc gtgattcaca agcgcagcca gcgccggcgc atcgaaggcc
    16621 gtcgcggtgc ggacgctgat agctagatcg ggttgcacga tcgatcgcgt atcagaagcg
    16681 tccgacattc agcgcgcgat cacaacttct tgcggcctga tccgcgggtc cgatcccgtg
    16741 cgtcgatttc gcttgcactg cttcgcgcga tttttccgcg cgagcgcgag ctacgcagtc
    16801 aggtgctgtt gcttcgcgcg ctgatcgcgc gttgctttgc gattgcgatc ggcgagcgcg
    16861 acgtgaaaca gcacgatcac ggttcccacc gtcacatcgg agactgcttt caccatccgg
    16921 tcagccaaat tccacatagt catccctcaa tgccgaagcc aatgtgcacc atgaacttcg
    16981 acgggacaac ctgaattccc tcgatgatcg aagaaaagaa atctgttacg cagctcgcgc
    17041 gttacagaga gagtgaagaa gtttttcgcg ttcttgatcg cgtgctcatc agcgcaatct
    17101 gcgtcgccat caccgtcgag cgtgacgcat gcgcacactc caacgtcacc tcgcgcaaca
    17161 acggttgcag ctcgaagtga caaaagcgaa acgaacgcga atttcggcga tgcagcggtg
    17221 acgagtcgcg tcaacgccgt tcatatcgtc gcgtccgtcg acggtgaatc cgcgaacgat
    17281 tcacccgcgt atgcacgcgc gaatcaaacc gtgaagctct tcgcagttat tcgcgcgggt
    17341 gacaagttct attccgatgc gccatcgccg agcctcggcg gcaagcgcat cactgtcgag
    17401 ccaatagcgc gtgggcctgt gatcgagctc gcgtggtcgc gcatcgaacc gaccacagcg
    17461 aacctttcga acactgcgag cggaactttc cgctttgaac cgatcgatta ccgcgcaacc
    17521 gagattgctg acggccgtgc tcgtacgtgg atccacgtag acgtgcggcc gacgctcacg
    17581 accgaccacg gcaacggcgt cggtacgatg cgttaccagc tcgcggttac ccaaggtgac
    17641 cacacgctga ccacgcccgg tcccgaagct cggcgcggcc gtggctcggg tgggctcacg
    17701 gatggagtga tgcgcgtatc gattcgccgc gacgacagct acctcggctt tctcaccgag
    17761 atgtacgggc aaccgtacgt gtgggcgtcg gccggcctct ccgatacaag tcatcagagc
    17821 gagcgactcg aaggctccga ctgcgcggac ctcatggtct atggcgcgcg acgcatgggc
    17881 aagcacgtcg cgtacacgtg gacgggcgga ctgccgaagg tgacgaagct aatcgcggcg
    17941 ggcacgcgcg acgagcacgg tgtgtatcgc gatgcgaacg gcaagccggt gccatacacg
    18001 caggaaggtg acctcgtgtt gttcccgcgt cacgtcggcg cgctcgctgc cgacaaaggc
    18061 gagatgggcg tgctcgacga tcaggacctg atgctgcaca cgctgttcga ctcgccgaag
    18121 gagcagccga tcaaggacag cggttacgcg cagtacccgg tcgagatccg gcggtttcgc
    18181 tagaccaggt cttcgtccgc gtcgatcacg aacgcgatca ggttcgcgag cggcggatga
    18241 aatgcggtcg agccgaacgg caacagtgcg ccgagctcgt cggcgtgcag cacctgctcg
    18301 agcgtgagcg ggcgcatcac catgccgacg gccgcgccgt tcgcgacggc atcgacaacg
    18361 ccggccgcgt cgagctgggt cgtcgacgcc gcggtctgca gcaccttcgc gaggaacatg
    18421 tgctcgacga ccaccggatc gagcttctgg atcgcgcggt ggacactcac gccctcggtc
    18481 accgggctga tatcgggctt gagcgtgagc ttccaggcgt cgccgtcgcc cgcgaagatc
    18541 gcgacgaacg cgggctgatg cgcgaccgtt tcaccgagcg cggactggac cttgtcgaca
    18601 tcgcgcgccg cgcccgcgat cttctcgatg atgaagtact tcttcgcgga ctcgagcacg
    18661 ttgtcgcgct tgatgccgtc gttgcgcacg agccggtggc gcgccgcgac gacgagcgcc
    18721 ggatcctcga ggttgacgag cgtcgcgagc ccgtacttcg tcgacgagta ttgcagctgg
    18781 ttttcagcgc cgagcttgtg cgcgtacgcg agcatgcctt cgtagcgcgc gtgaccgtcg
    18841 agcacgtgca gcttcttcgg cgcgaactgc gtgcgcacct tgccgatcac ctcggcgttc
    18901 gcgacgcgcc acagtcgatg gatggtgccg tcgggcgtcg tgacctcgac ggtcgggcgc
    18961 cccgactcga caccgcgaaa cagacggtcg acttcacgtg cggcgtcgcg atagcccgcg
    19021 aacacggcct cggtatgcgc gccggcggcg ttgatcgcgg ccgtcgccgc atcgcgtgcc
    19081 gtcggatcgg tctgctcgtg cgggcggatc gtgccttccg tccacggctc gagccgcaac
    19141 gcgcacacga ccgtctgtcg cgtcagcgag cggccgccga gcgtgaacgt ttggtgatag
    19201 cgatacagcg cgcgcgacgg atcgcgaacg agctcacccg ccgcgagtcg ttccttcacg
    19261 ccggtcaccg ggctcgcaac aacgctgtcg agttggacct tcgaaccgtc ccacagcgcg
    19321 ccacggaacc ctgcaatctg cggcatacga gaatcgtagc ttatcggcgc gcgccgatgt
    19381 cgccgcggct ctgcttgccc tcgagctgca ccgcatcgac gactgcgtat gcccgctcac
    19441 gcgcctgctg gacgctgacg ccgagcgccg tcactccgag tacccgacca cccgcggtga
    19501 cgagcttgcc gtccttgcgt gcagtgccgg cgtggaacac gacgacatcc tcgctaaccg
    19561 aatcgaggcc gccgatcacg tcaccggtgc ggacgctccc gggataaccg gccgcggcca
    19621 cgacgacgca caccgccacg cgcgcgtccc aggcaagctt gccgaccggt atctcgcccc
    19681 gcgcggcacc cgccagcacc gcgccgaggt cgccgatcat gcgcgccatc agcggttgcg
    19741 tctcgggatc ac
//
LOCUS       DASUFX010000404         6081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_343448,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000404 DASUFX010000000
VERSION     DASUFX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343448"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2185)
                     /locus_tag="VFV99_16190"
     CDS             complement(<1..2185)
                     /locus_tag="VFV99_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060909.1"
                     /translation="MGVPDDPSTELLPAGAESAAAPSTTPTTSLIGRTLGRFVIMERL
                     GKGGSGEVFRAQQVQLGRSAVIKVLRREVAAVPNRVERFLREAKLASRLDHPYAAHIY
                     AFGVEPDNVMWIAMEHVKGITLDELVSRRGAMPASVFAPLFARLCEVVHSAHELGIVH
                     RDIKGANVMVIERAGQLLPKLLDFGIAKADSDDAAPGVDDGELTGHGVTLGSPHYMSP
                     EQWERPSEVDGRSDIYGLGVLAYRCLSGHLPFHTLDRTQLSHAHTKQPVPPLPEHVPP
                     QLSAVVMRALAKNPDERWQTAVELADGIQRVSNAPSAEVVPIFDPTTRDAWLRAGPQP
                     IADAMAHLTSATTTVEADAALTELVAITCRWLAVLALAGLPETNDPSVREQARAVVGR
                     DDGAPWLRLAKAAVAAAKEPLPALVAALAASEPLSELADRLDDRDRTRTAAMLATNVA
                     AAADALRGLEPLLAYQLVVGASGAIAESWQGPRRRERDRVLVWGEPLDEMQVALLDSG
                     GKVVAKLSPYAQVISPFPAAEPELFLLWRTGRGQARLMAAPWGFERDDDAAGMKLAAL
                     STEDSDTAADPADDRSPYPGLASYSVADAEHFVGREREVEALANRLVRAPLVAVLGPS
                     GVGKSSFIHAGLVPRLGEHYRILTMRPGRHPMHALASLPPVSSDSQDEGALVSRLREL
                     GESAQRGLVLVIDQLEELVTLCADPKERTRFADTLAAVADGSSAPV"
     gene            2300..3244
                     /locus_tag="VFV99_16195"
     CDS             2300..3244
                     /locus_tag="VFV99_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060910.1"
                     /translation="MRAFVILAAFLIVACGPSNRGDDDGNGNNPDSGNNGSGSGSGSG
                     NEDGCSDAAKLIYVVDSNNTLSSFDPATKTFKDLGTLSCPAASGAQPFSMGVDRNATA
                     YVLYSGVNPLSGDVTSTEIFKVDTTQSGLPCTKTTFAGTADFKQFGMGFSTDAAMGDT
                     DTLFIAGSPGVGTGATKLGKFDLSTMTPSTIGTVTGDPELTGNGNAELWAFFPSDTTG
                     ATDPKVGKLDKATGALSSPYTLTALKGIPAAWAFAFWGGDYWIFLAKSSSGPIPTPGK
                     TVVYQVDGSNGTVKGMTDTMTRTIVGAGVSTCAPVVIL"
     gene            3346..4218
                     /locus_tag="VFV99_16200"
     CDS             3346..4218
                     /locus_tag="VFV99_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060911.1"
                     /translation="MRAFVLLAAFLIACGPDTRGDDDGMGSGSGSGSGSGSGNMDGCS
                     DAAKLVYVVDENNKLSKFDPSTKTFMDLGTLNCPAQFLATPFSMGVDRNAIAWVLYNS
                     GELFRVDPSNLGCTKSTWATQLGLTVFGMGFSANVKGSTDDTLFIGGGGDQTSQNATL
                     ATLSTTSFQATRVGNITGWPELTGNGNAELWGWFPSDSTGGTTPRVEKIDKTTGAALT
                     TYMLPTLQGAPMAWAFAFWGGDYWIFLAKGTETNTTVYQIDGTNGTIKSTTPAAGRLI
                     VGAGVSTCAPVVIL"
     gene            4249..5307
                     /locus_tag="VFV99_16205"
     CDS             4249..5307
                     /locus_tag="VFV99_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060912.1"
                     /translation="MPDPKQDSKSGVEDGWDDQPAQQPAHRPQKPSRPHLPGVNPKPG
                     QDQSSLALLAKDLMPPPAASQAPTQIDVNTVGNASDRKVQDPGLKIKLKSFVRRWSGK
                     LTSFVKNRKLVAISLAGTIVIVLVLIIVLLAGGGGSKQSRGAKLAKQGQELLNANDAK
                     HAAELIESELVGAAQAGDGEAYLVLGHARFAMKRYLEAVTAYERALTLSPKLASDKVL
                     RGNATKVLESKDSVAAVVALELLASRVTPPANDVVVQYASTGKLQDARRRAFAIAERE
                     GIADKIDRVESWSLDLAAAGTCEDRRTIVAKLASTKDKRAIPALKKMKLYKCAEKDAT
                     DAIAEIEGGGAAGSAEPK"
     gene            complement(5308..>6081)
                     /locus_tag="VFV99_16210"
     CDS             complement(5308..>6081)
                     /locus_tag="VFV99_16210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060913.1"
                     /translation="PRTDTDFTVAKLTANGALDATWGTSGRFTLDIQESNATARALHV
                     LGDGSVIAGGYANSPGVGTTQPVLYRLTPAGALDANFATGGLFHETVLTLQTEVYNFA
                     IDGNKLTTAGYGRNAGTTNDWVALRFNTTTGARDTNFGNAPNGAVMIDPSGMMLGSNC
                     RNALALPGGKTALIGSTGPSNMPAQDGAVAILDVNGKLDTHFGTGVNVFPLGPSSNDQ
                     FWSGAVASNKLLVTGWRGGGMTQTDTVNDDAHAVVFELD"
BASE COUNT         1051 a         2005 c         2072 g          953 t
ORIGIN      
        1 gcaccggcgc actcgaacca tcggcgaccg ccgcgagcgt gtcagcgaac cgcgtacgct
       61 ccttcggatc ggcgcacagc gtgacgagct cctcgagctg atcgatcacg agcacgagac
      121 cgcgctgcgc actctcgccg agctcgcgca gacgcgagac gagtgcgccc tcgtcctggc
      181 tgtcgctcga caccggcggc aggctcgcga gcgcgtgcat cggatgacgg ccgggtcgca
      241 tcgtcaggat gcggtagtgc tcgccgagac gcggcacgag cccggcgtga atgaacgagc
      301 tcttaccgac ccccgatggc ccgagcacgg caaccagcgg cgcgcgcacg agccgatttg
      361 cgagcgcttc gacttcgcgt tcgcggccga cgaagtgctc ggcgtcggcc acgctgtacg
      421 acgcgaggcc ggggtacggc gagcgatcgt cggccggatc ggcggcggta tccgagtcct
      481 ccgtcgacag tgcggcgagc ttcatgcccg ctgcgtcgtc gtcgcgctcg aagccccacg
      541 gcgctgccat caggcgcgcc tgcccgcggc cggtacgcca cagcaagaac agctcgggct
      601 ccgccgccgg gaatggcgag atcacctgcg cgtacggcga cagtttcgcg acaaccttgc
      661 cgccggaatc gagtagcgcg acctgcatct cgtcgagcgg ctcaccccac acgagcacgc
      721 ggtcgcgctc gcggcgccgc ggtccctgcc agctctcggc gatcgcgccg gacgcaccga
      781 cgacgagctg atacgcgagc agcggctcga gcccgcgcag tgcgtctgcc gccgcagcga
      841 cattggtcgc gagcatcgcc gccgtgcgcg tgcgatcgcg atcgtcgagt cgatcggcaa
      901 gctcactgag cggctccgat gcagcgagtg ccgcgacgag cgctggcaac ggctctttcg
      961 cggccgcgac ggctgccttc gcgaggcgca gccacggggc gccgtcatca cggccgacga
     1021 ccgcgcgcgc ttgctcgcgc accgacggat cgttcgtttc cggcaagccc gcgagtgcga
     1081 gcacggcgag ccagcggcac gtgatcgcga cgagctcggt gagcgcggca tctgcctcga
     1141 cggtcgtcgt cgctgacgtc aggtgcgcca tcgcatcggc gatcggctgc gggccggcgc
     1201 gcagccacgc gtcgcgcgtc gtcggatcga agatcggcac gacctcggcg ctcggcgcat
     1261 tcgatacgcg ctggattccg tccgcgagct cgactgcggt ctgccagcgc tcgtcgggat
     1321 tcttcgcgag cgcgcgcatc acgaccgccg acagctgcgg cggcacgtgc tcgggcagcg
     1381 gtggcaccgg ctgtttcgtg tgcgcatgcg agagctgcgt gcgatcgagc gtgtggaacg
     1441 gcagatgacc ggagaggcag cgatacgcga gcacgccgag cccgtagatg tcggagcgcc
     1501 cgtcgacttc ggatggccgt tcccattgct cgggagacat gtagtgcggc gagccgagcg
     1561 tgacgccgtg gccggtgagc tcgccgtcgt cgacgccggg cgcggcatca tcgctgtctg
     1621 ctttcgcgat gccgaagtcg agcagcttcg gcaacagctg gccggcgcgc tcgatcacca
     1681 tcacgttcgc gcctttgatg tcgcgatgca cgatgccgag ctcgtgcgcg gaatgcacga
     1741 cttcgcagag ccgcgcgaac agcggcgcga acacgctcgc cggcatcgcg ccgcgccgcg
     1801 agacgagctc gtcgagcgtg atgcctttga cgtgctccat cgcaatccac atcacgttgt
     1861 cgggctcgac gccgaacgcg tagatatgcg cggcgtacgg atggtcgagg cgcgaggcga
     1921 gcttggcttc gcggaggaag cgctcgaccc ggttagggac cgcggcgacc tcgcgccgca
     1981 acaccttgat caccgccgag cggccgagct gcacctgttg cgcacgaaag acctcacccg
     2041 agccgccctt accgagccgc tccatgatca cgaagcggcc taacgtgcgg ccgatcaagg
     2101 aggttgtggg cgtggtactc ggagctgccg ctgattctgc gccagcaggc agcagctccg
     2161 tcgacggatc gtcgggaacc cccacgggca gaactgtatc gccgccgctg gtagacgaaa
     2221 accccgcaca tcactcaact caggcccggt cagaacgcgg ctgctggatt ttttggtcaa
     2281 agtgatagcg tccgtcgcga tgcgggcatt cgtcattttg gcggcttttc tgatcgtcgc
     2341 gtgtggtccc agtaaccgcg gcgacgatga cggcaacggc aacaaccccg actccggtaa
     2401 caacggcagc ggcagcggca gcggcagcgg caacgaagac ggctgcagcg acgcggcgaa
     2461 gctgatctac gtcgtcgaca gcaacaacac gctgtcgtcg ttcgacccgg cgacgaagac
     2521 gttcaaggac ctcggcacgc tgagctgccc ggccgcgagc ggcgcgcaac cgttctcgat
     2581 gggcgtcgat cgcaatgcga cggcatacgt cctctacagc ggcgtcaacc cgctcagcgg
     2641 tgatgtgacc tcgaccgaaa tcttcaaggt cgacacgacg cagtcgggcc tgccgtgcac
     2701 gaagacgaca ttcgccggca cggcggactt caagcaattc ggcatgggct tctcgacgga
     2761 cgccgcgatg ggcgacaccg acacgttgtt catcgccggc agcccgggcg tcggcacggg
     2821 cgcgacgaag ctcggcaagt tcgacctctc cacgatgacg ccgtcgacga tcggtacggt
     2881 caccggcgat cccgagctca ccggcaacgg taacgcagag ctgtgggcgt tcttcccgag
     2941 cgacacgacc ggcgcgacgg atccgaaggt cggtaagctc gacaaggcga ccggcgcgct
     3001 ctcgtcgccg tacacgctca ccgcactcaa gggcatcccg gccgcgtggg cgttcgcgtt
     3061 ctggggtggc gactactgga tcttcctcgc gaagtcgagc tcgggaccga tccccacgcc
     3121 tggaaagacg gtcgtgtatc aggtcgatgg ctcgaacggc acggtcaaag gcatgaccga
     3181 tacgatgacc cgcacgatcg ttggcgcagg tgtgtcgacg tgtgcgccgg ttgtgatcct
     3241 ctgatcacat aaagcgtaag tgcccacggc agttggcacg acgcgtccgc accggacgcg
     3301 aaaacgaacc ccggtacaaa aacggcccgt gatagcgtcg ggccgatgcg ggcattcgtc
     3361 cttcttgcgg cttttctgat cgcgtgtggt cccgatacgc gcggcgacga tgacggcatg
     3421 ggcagcggca gcggctcggg cagtggctcg gggagcggca acatggatgg ctgctccgac
     3481 gcggcgaagc tcgtctacgt cgtcgacgag aacaacaagc tgtcgaagtt cgatccgtcg
     3541 acgaagacgt tcatggatct cggcacgctg aactgcccgg cgcagttcct cgcgacgccg
     3601 ttctcgatgg gcgtcgatcg caacgcgatc gcgtgggtgc tctacaactc gggcgagctg
     3661 ttccgcgtcg atccgtcgaa cctcggctgc acgaagtcga cgtgggcgac gcaactcggc
     3721 ctcaccgtgt tcggcatggg cttctcggcg aacgtgaagg gaagcaccga cgacacgctg
     3781 ttcatcggcg gcggcggcga tcagacgagc cagaacgcga cactcgcgac gctgtccacg
     3841 acttcgttcc aggcgacgcg cgtcggcaac atcaccggct ggcccgagct gaccggcaac
     3901 ggcaatgcgg agctgtgggg ctggttcccg tcggactcga ccggtggcac cacgccgcgc
     3961 gtcgagaaga tcgacaagac gaccggcgcc gcgctcacga cgtacatgct gccgacgctg
     4021 cagggtgcac cgatggcatg ggcgttcgcg ttctggggtg gcgattactg gatcttcctt
     4081 gcgaagggca ccgagacgaa cacgaccgtc tatcagatcg atggcaccaa cgggaccatc
     4141 aagagcacga cgcccgcagc gggccgttta atcgtcggcg caggtgtgtc gacgtgtgcg
     4201 ccggttgtga tcctctagcg cgctgatcga tctacactcg atcgggtcat gcctgacccg
     4261 aagcaagatt cgaagtctgg cgttgaggat ggttgggatg accagccggc ccagcagccg
     4321 gcacatcggc ctcagaagcc gagccggccg cacttgccgg gcgtgaaccc gaagccgggc
     4381 caggatcaga gctcgctcgc gctgctcgcg aaggacctga tgccgccacc cgcggcttcg
     4441 caggcaccga cgcaaatcga cgtcaacacc gtcggcaacg ccagcgatcg taaggtccag
     4501 gacccgggcc tgaagatcaa gctgaagagc ttcgtgcggc ggtggagcgg caagctcacg
     4561 agcttcgtca agaaccgcaa gctcgtcgcg atctcgctcg ccggcacgat cgtgatcgtg
     4621 ctcgtgctca tcatcgtgtt gctcgcgggc ggtggcggta gcaagcagtc gcgtggcgcg
     4681 aagctcgcga agcaaggcca ggagctcctc aacgcgaacg acgcgaagca tgcggccgag
     4741 ctcatcgaga gcgagctcgt cggcgcagcg caggccggtg atggcgaggc gtacctcgtg
     4801 ctcggccacg cgcggttcgc gatgaagcgc tatctcgaag cggtcaccgc gtacgagcgc
     4861 gcgctcacgc tgtcgccgaa gctcgcgagc gacaaggtgc tgcgcggcaa cgcgacgaag
     4921 gtgctcgagt cgaaagactc ggtcgcagcg gtggtcgcgc tcgagctgct ggcttcccgg
     4981 gtaacgccgc cagcgaacga cgtcgtcgtg caatacgcgt cgactggcaa gctgcaggat
     5041 gcgcggcggc gcgcgttcgc gatcgccgag cgcgaaggta tcgccgacaa gatcgatagg
     5101 gtcgagagct ggtcactcga tctcgcggcc gccggcacgt gcgaagaccg ccgcacgatc
     5161 gtcgcgaagc tcgcgagcac gaaggacaag cgcgcgattc cggcgctcaa aaagatgaag
     5221 ctctacaagt gcgccgagaa agacgccacc gacgcgatcg cggagatcga aggcggcggc
     5281 gctgccggca gcgcggagcc gaagtagtca gtcgagctcg aacacgaccg cgtgcgcgtc
     5341 gtcgttgacg gtgtcggtct gcgtcatacc gccgccgcgc cagccggtga cgagcagctt
     5401 gttgctcgcg actgcgccac tccagaactg atcgttgctg gagggtccga gcggaaacac
     5461 gttcacgccg gtcccgaagt gcgtgtcgag cttgccgttg acgtcgagga tcgcgacggc
     5521 tccatcttgc gcgggcatgt tgctcgggcc cgtcgagccg atcagcgcgg tcttgccgcc
     5581 aggcagcgcg agcgcgttgc ggcagttact gccgagcatc atgcccgatg gatcgatcat
     5641 gaccgcacca tttggtgcat tgccgaagtt cgtgtcacgc gcgccggtcg tcgtgttgaa
     5701 gcggagcgcg acccagtcat tcgtcgtgcc ggcgttgcgg ccgtagccgg cggtcgtcag
     5761 cttgttgccg tcgatcgcga agttgtagac ctccgtctgc agcgtgagca cggtctcgtg
     5821 aaagaggccg cctgtcgcga agttcgcgtc gagcgcgcca gccggcgtca accggtacag
     5881 caccggctgc gtggtgccga cgccgggcga gtttgcgtag ccgcctgcga tcacgctgcc
     5941 atcgccgagt acgtgcagcg cccgcgcggt cgcgttcgac tcttgaatgt cgagcgtgaa
     6001 cctgccgctg gtgccccacg tcgcgtcgag cgcgccgttc gcggtcagct tcgcgaccgt
     6061 aaagtcggtg tcggtgcgcg g
//
LOCUS       DASUFX010000405         9748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_204889,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000405 DASUFX010000000
VERSION     DASUFX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204889"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFV99_16215"
     CDS             <1..1108
                     /locus_tag="VFV99_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5060914.1"
                     /translation="IVAFLGASTAGAGVAHADRALPMDNSIADVAERVVESVVNIDTE
                     SQVATDPFFVDMFGADEVQKMAGKGSGVIVTANGRILTNAHVINGADTIMVTLHDGTE
                     VKAKLVGKDTKADLAVLQLEGKFGSLKPLPMGDSSQTRLGELVLAIGDGLGIGKSVSL
                     GIVSAKGRGLKLAEYEDFIQTDAAINPGNSGGALVNMKGELVGINTAIASRTGGYQGI
                     GFAIPTNMAKPIMEALMKDGKVTRGYMGIGIATVSPPIAKKYGLGAQQGVLVASVEAS
                     GPAAKAGLVEGDVITSFNGNAVKSDDVLRNQIAALKPGSTVEVAIAHRDGKTATVKAK
                     LGELKDEEPPMARSKKQRQQQPQRKSQQWQWQSP"
     gene            1153..1527
                     /locus_tag="VFV99_16220"
     CDS             1153..1527
                     /locus_tag="VFV99_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060915.1"
                     /translation="MLRLALFVCVLGGCISKAKEAIAPAPDSVGTLSCREVVEQCDSQ
                     CGDPLCINRCTGEGNQEAQGQHAALLDCGQSHGCTDQACMEQNCPSEIATCMGPPSGS
                     PADTPTAPANEPTGAPGQTPPG"
     gene            1690..3504
                     /locus_tag="VFV99_16225"
     CDS             1690..3504
                     /locus_tag="VFV99_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060916.1"
                     /translation="MTSLNETTPFARDDEPTGEPFGPYVVYEKLGEGGMAYVHRAELV
                     GDNGLRKPVALKRLLTNAAEDPDFVASFVHEAQLAAKLKHPNIVQAYDLGKLDNTYYI
                     AMELVPGPTLAQVMTQAHNGAGAVPLPIALEILIQLCDALDHAHDLRDDGGRRLDLVH
                     RDVNPMNVIVSRTGVVKLIDFGIAKARSASRVTEVGIIKGKHAYVAPEYTYGKLDHRA
                     DLFGLGVLAHELLTGRRLFLGETDLQTIRNVREMIVPPPSRFAPDISTQLDAIVLKAL
                     ERDPNERWQTANEMRTALTEERHRLGVAITGRQIREWAEWAFQQAPRRETSINRMLDG
                     LEPSISIERPLESTSERAETMQGTGVKTALRQPTAPTVPIGRLSEDDVLTEAVLPRVA
                     NDDDVPTEALLPANDDDMPTVDQRAPTPVPVRLHRSAALIPNRPATVARSLADECPPV
                     PRALAATAVAPAPKPRALLVATPATGSRAYLVPAPAPVVAKPLVVANGTGPTSVPNEQ
                     LAVGSGPTARPPVRVGPALVVTTPVVAKAPAAAQPAKRRPRAPAWRPPTRWAHPQKRR
                     SPAPFFILLGLIGLAAVLVSQGYIDIERWREIIATYIG"
     gene            complement(3721..5487)
                     /locus_tag="VFV99_16230"
     CDS             complement(3721..5487)
                     /locus_tag="VFV99_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060917.1"
                     /translation="MVRWLGARYAPWVVLAIGIALAAPAITANFTADDHLHRVVERDD
                     TGIAGLHPRSLDLFTFANGNVADTEQLRDGGLFPWWVDPHLKLSFMRPLSSVTHVLDH
                     ALWPDNAAAQLAHNLVWLALSLVFAWLFFRRFLAARWIAVLALALYAFDDARGPVVGW
                     IANRNALVALCLAMPVLLAHDRWRREGWQPGRWLAPSLFVVALGAGESSVAILAYVAA
                     HALWLDRGSWRDRALALAPYVFLVVAWRVIYAKLGYGVFGSGIYLDPGSDPLAFLGAA
                     AQRLPFLLAGALALPWSDFASFYPVLGVLAVMLAIALVLLGLIAFACARLLRRDPTAR
                     FFATGMLLAAIPVASTFPADRLLTFVCLGGMGLIAQLLATAVRDRAQLGDSKLRRFGA
                     TAVAVLMLVLHLVLAPPFLVLRARSMVAVARVLDRADASIPGNAHTVLIVTTPSDALV
                     GFVPLMRHSRGQPCPDHLYWLSTATTPVTLERLDDKTLRVSPERGFLLHEVDQMMRSA
                     RIRPFAVGDRIPLSGLTMEIESVTPDGRPLTALAHFATSLDDPSLTWLRWEGHAYVAY
                     RPPAVGAREPLPAVDFSKLLGD"
     gene            complement(5501..7753)
                     /locus_tag="VFV99_16235"
     CDS             complement(5501..7753)
                     /locus_tag="VFV99_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5060918.1"
                     /translation="MGKLSWWPAQGTIDLAHHDLPHPAADTEWWYVNSHLRLADGRTF
                     GLFAAFFRIINAVDEKTGAVEYAHSYTWALSDLDRGSYHGESRVDQRAPELGLQRLAN
                     GRGAKDDRLNRAMKEILDRGQVPAPDRVFDGPVHIASDRLHVQYGSGSFEKQADGSYR
                     VQLVHDDKQTACELVFAPQKQPIRHGDDGVVRGNAGEQMFYYFIPRCTVTGTITVGGE
                     THPVAAAQGWYDHEFGGYVADDTKADAAAQGEGAGIRDLGWNWTAIQLDDGTDLTAYE
                     IIDVKSHETVGKWMIGIDADSKRRSIDDVTFKPVKTWTSHRTFHDYPTSWRLTAPSAG
                     IDLTIEAAFPDQELITVISKPAFWEGRCTVKGTANGRQVSGLAYIERSGFENIETLDD
                     FFSAVGKEVRRSTAQALPLEPTHDELRDLIASEELAHYMDGVDRPSMAETLIKPIREM
                     VDRGGKSWRSYAALACCDVVGGDSRKFSQWLAMPELLHVGSLIVDDVQDKSTVRRGGP
                     CTHVTYGEPIAINSGTAAYFLTQNQLHSEELNDKQKLRIYDLYFAAMRAGHAGQAIDL
                     GGMASLMPRVVETGDSTELAGRILACHRLKTAAPAGCLARMGAVAGGGTDEQIAAVGE
                     FFEAVGLAFQIMDDVLNLNGFKGNLKQRGEDIANGVVTLPIAYAMSRLPKDQREHLWR
                     TLQSKPTDIATIAATIELVQSCGAIKACADQARELVEAGWRKAEPLLDPSLQRLMLRA
                     FGWYVLERHY"
     gene            7880..8482
                     /locus_tag="VFV99_16240"
     CDS             7880..8482
                     /locus_tag="VFV99_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060919.1"
                     /translation="MTLTIETLAAEPRWELAKVQALPGRSRGDLLGSWGSNVMKRFGS
                     TGLQRVLRRLPSPLDALPATLGAKDWVPVYAQLLVTEAIVDEYLGGDMRALYPLLVED
                     TRAGIGSVQLVVLKAMGPVRALKLAPRTFRHVHEQGSVDAEVGGRSARMTFSGSPLFT
                     HPTWRLLQLFATHTMLELTGRRGSSSGETMTNGFVANTNW"
     gene            8529..9608
                     /locus_tag="VFV99_16245"
     CDS             8529..9608
                     /locus_tag="VFV99_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060920.1"
                     /translation="MKQLWPIVLTLGGCIISSGPSDDIDQTQADVDHYNQLSAELDAR
                     RSEFLGRDVQELAAAGSTLYWLDTTNFNPKLMHYDGQTKLAYGFSIGDGNNYNYRASA
                     SVVVTAEPGSDAVIYRAYDAVAANRLLGTTSLPKPVGAKWSAYAVDSSTVYIMDGTTL
                     KKWLPGGDVTTVTTLESAGVEAGEFWDFGVASNTMVFIESGRVWSMDLAANHATWLMN
                     MTEAGGTVDFRPDGVLFDTATGLSFYDYAQRKLIDIRAAIDANPFKINNTFASAAKFD
                     TDFTRYKQWVLYIGQSGLFAYDFKADKITPLLLSPNRADLRVDYRYPVALDTGVAFVT
                     GLTSTSGATGADGPTYTLDLKPILH"
BASE COUNT         1745 a         3158 c         3288 g         1557 t
ORIGIN      
        1 gatcgtcgct tttcttggtg cgagcaccgc tggtgcaggc gtcgcgcacg ccgatcgagc
       61 gctgccgatg gacaactcga tcgctgatgt agccgagcgt gttgtcgaga gcgtcgtcaa
      121 catcgatacc gagagtcagg tcgcgaccga tccgttcttc gtcgacatgt tcggcgcgga
      181 cgaggtgcag aagatggccg gcaaaggctc cggcgtcatc gtcaccgcga atggtcgcat
      241 tctgacgaac gcgcacgtga tcaacggcgc cgacacgatc atggtcacgc tgcacgatgg
      301 caccgaggtc aaggcgaagt tggtcggcaa ggacaccaag gccgacctcg cggtgcttca
      361 gctcgaaggc aagttcggtt cgctgaagcc gctgccgatg ggcgactcgt cgcagacgcg
      421 cctcggcgag ctcgtgctcg cgatcggtga cggtctcggc atcggcaaga gcgtgtcgct
      481 cggcatcgtg tctgcgaagg gccgcggcct caagctcgcc gagtacgaag acttcattca
      541 gaccgacgcc gcgatcaacc cgggcaactc gggtggcgcg ctcgtcaaca tgaagggcga
      601 gctggtcggc atcaacaccg cgatcgcgtc gcgcaccggt ggctaccagg gcatcggttt
      661 cgcgattccg acgaacatgg cgaagccgat catggaagcg ctgatgaagg acggtaaggt
      721 gacgcgcggc tacatgggca tcggcatcgc aacggtgtcg ccgccgatcg caaagaagta
      781 cggcctcggc gcgcagcaag gcgtgctcgt cgcgagcgtc gaagcaagtg gcccggcggc
      841 gaaggccggc ctcgtcgagg gcgacgtgat cacgtcgttc aacggcaatg cggtgaagag
      901 cgacgacgtg ctccgcaatc agatcgcggc gctcaagccg ggcagcaccg ttgaagtcgc
      961 gatcgcgcat cgcgatggca agacggcgac ggtgaaagcg aagctgggcg agctcaagga
     1021 tgaagagccg ccgatggcgc gcagcaagaa gcagcgccag cagcagccgc aacgcaagtc
     1081 gcaacagtgg cagtggcaat cgccttgatc gcggcgagcc actgacgctc caatcagcgc
     1141 tactctcggc gaatgctgag actagcgctt tttgtttgtg tgctcggcgg ctgcatcagc
     1201 aaggcgaagg aagcgatcgc gcctgcgccc gacagcgtcg gcacgctcag ctgccgcgaa
     1261 gtggtcgagc aatgcgatag ccagtgtggc gatccgctct gcatcaatcg ctgcaccggc
     1321 gaaggcaatc aggaagcgca ggggcaacac gctgcattgc tcgactgtgg ccagagccac
     1381 ggctgcaccg atcaggcgtg catggagcag aattgcccgt cagaaattgc cacctgtatg
     1441 ggcccgccga gcggatcacc cgccgatacc ccaacggctc ccgcgaacga gccgacgggc
     1501 gcgcctggac aaaccccgcc aggctgagca gttagcgagc cgttgtgcga ttcgcgcact
     1561 ggctactcga gttgccaaca ggaccttaca agcggtcgct tcagcgaccc gaattggttc
     1621 gttgttgtct gccagtcaat ttgccaacgt tccaccctga tggtcgctcc gcattcaccg
     1681 tcgatgcgca tgacgtctct gaacgagacg acgccgttcg cgcgcgacga cgaaccgacg
     1741 ggcgaaccat tcggtccgta cgtcgtttac gagaagctcg gcgaaggcgg catggcgtat
     1801 gtccatcgcg cggagctcgt cggcgacaac ggtctgcgca agccggtcgc gctcaagcgc
     1861 ctgctcacga acgctgccga ggatcccgac ttcgtcgcgt cgttcgtgca cgaagcgcag
     1921 ctcgcggcga agttgaagca cccgaacatc gtgcaggcgt acgacctcgg aaagctcgac
     1981 aacacgtact acatcgcgat ggagctcgtg cccgggccga cgctcgcgca ggtgatgacc
     2041 caagcgcaca acggcgccgg cgcggtgccg ctgccgatcg cgctcgagat cctgatccag
     2101 ttgtgcgacg cgctcgatca cgctcacgat ctgcgcgacg acggcggtcg tagactcgac
     2161 ctcgtccatc gcgacgtcaa tccgatgaac gtgatcgtct cgcgcacggg cgtcgtgaag
     2221 ctcatcgact tcggcatcgc gaaggcgcgc tcggcgtcgc gcgtcaccga ggtcggcatc
     2281 atcaagggca agcatgctta cgtcgcgccc gagtacacgt acggcaagct cgatcatcgc
     2341 gccgacctgt tcggcctcgg cgtgctcgcg cacgagctgc tcaccggccg ccgcttgttc
     2401 ctcggcgaaa ccgatctgca aacgatcagg aacgtccgcg agatgatcgt gccgccgccg
     2461 tcgcgcttcg cccccgatat ctcgacgcag ctcgatgcga tcgtgctgaa ggcactcgag
     2521 cgcgatccga acgagcgctg gcaaaccgcg aacgagatgc gcaccgcgct caccgaggag
     2581 cggcaccggc tcggcgtcgc gatcaccggt cgccagatcc gcgagtgggc cgaatgggcg
     2641 ttccagcagg ccccgcgccg cgagaccagc atcaaccgta tgctcgacgg cctcgagccg
     2701 agcatctcga tcgagcgtcc gctcgagtct acgagcgagc gggcggagac gatgcaaggc
     2761 accggcgtga agacggcgtt gcgccagccg acggcgccga ccgttccgat cggtcgcctc
     2821 tccgaagacg acgtgctcac cgaggcagtt ctgccgcgcg tggcaaacga tgacgacgtg
     2881 cccaccgagg cgttactgcc ggcgaacgac gacgacatgc cgacggtcga ccagcgcgcg
     2941 ccgacgccgg tgccggtcag gctccaccgt tcggctgcac tcatcccgaa tcgcccggcc
     3001 acggtcgcgc gctcgctcgc agacgaatgc ccgccggttc cgcgcgcgct cgccgcgacg
     3061 gccgtcgcac ctgctccgaa accgcgggca cttctggtgg ccacgcccgc cacgggatcc
     3121 cgcgcgtatc tcgtgcctgc gcccgctcca gttgttgcga agccactcgt cgtcgcgaac
     3181 ggcactggcc cgacctcggt gccgaacgag cagcttgccg tgggcagcgg cccgacggcg
     3241 cggccacccg tgcgcgtcgg ccctgcgctc gtggtgacga cgcctgtcgt cgcgaaggcg
     3301 cctgccgccg cgcagcccgc gaagcgccgc ccgcgtgcac cggcctggcg accgccgacg
     3361 cgctgggcgc atccgcagaa gcgccgctcg ccggcaccgt tcttcatcct gctcggcctg
     3421 atcggcctcg ccgcggtgct cgtctcgcag ggctacatcg acatcgagcg ctggcgcgag
     3481 atcatcgcga cgtacatcgg ttagtcgccg agtagcttcg agaagtcgac cgcaggcagc
     3541 gtttcgcgcg cgccgaccgc aggcgggcgg tacgcgacgt acgcgtggcc ttcgaagcgc
     3601 aaccacacca gcgacggatc gtcgagaatc ggacagggtc agacatgtct tgtatgcgtt
     3661 gctcggacag ctgtctggga acgcatgcaa cgcatgtctg accctgtccg ggacatgtac
     3721 ctaatcgccg aggagcttcg agaagtcgac cgcgggcagg ggctcgcgtg cgccgaccgc
     3781 aggcgggcga tacgcgacgt acgcgtggcc ttcccagcgc agccacgtca gcgacggatc
     3841 gtcgagggac gtcgcgaagt gggcgagcgc ggtgagcggg cggccgtcgg gtgtgacgga
     3901 ctcgatctcc atcgtgagac cggagagcgg gatgcggtca ccgaccgcaa acggacggat
     3961 gcgtgccgag cgcatcatct gatcgacttc gtggagcaag aaaccgcgct cgggcgagac
     4021 gcggagcgtc ttgtcgtcga gccgctcgag cgtgaccggc gtggtcgccg tcgagagcca
     4081 gtagagatga tcggggcagg gctggccgcg cgagtggcgc atcagcggga cgaagccgac
     4141 aagcgcgtcg gatggcgtcg tcacgatgag gaccgtgtgc gcgttgccgg ggatcgaagc
     4201 gtcggcgcga tcgagcacgc gggcgaccgc gaccatcgag cgcgcgcgga gcacgaggaa
     4261 cggtggggcg agcacgaggt ggagcacgag catgagcacc gcgaccgcgg tcgcgccgaa
     4321 ccggcggagc ttgctatcgc cgagctgggc gcggtcgcgg acggccgtgg cgagcagctg
     4381 cgcgatgagg cccatcccgc cgagacagac gaacgtgagc agccgatcgg cggggaacgt
     4441 gctcgcgacc gggatcgccg cgagcagcat gccggttgcg aagaagcgcg cggttggatc
     4501 gcggcggagc aggcgagcgc acgcgaatgc gatcaggccg agcagaacca gcgcgattgc
     4561 gagcatgacg gcgagcacgc cgagcaccgg atagaacgac gcgaagtctg accacggcag
     4621 cgcgagcgca ccggcgagca agaacggcag tcgctgcgcg gcggcaccga ggaacgcgag
     4681 cggatcgctg cccggatcga ggtagatgcc ggagccgaag acgccgtagc cgagctttgc
     4741 gtagatcacg cgccacgcga cgacgaggaa tacgtagggg gcgagtgcga gcgcgcggtc
     4801 gcgccagctg ccgcgatcga gccacagcgc gtgcgcggcg acgtacgcga ggatcgcgac
     4861 cgatgactcg ccggcgccga gcgcgacgac gaacagagac ggcgccagcc aacggccagg
     4921 ctgccagccc tcgcgccgcc agcggtcgtg cgcgagcagc acaggcatcg cgaggcagag
     4981 cgcgacgagc gcgttgcgat tcgcgatcca gccgacgacc ggaccgcggg cgtcatcgaa
     5041 ggcgtagagc gcgagcgcga gcacggcgat ccaccgcgcg gcgaggaagc ggcggaagaa
     5101 cagccaggcg aaaacgagcg acagcgcgag ccacacgagg ttgtgtgcga gctgcgccgc
     5161 cgcgttgtcc ggccacagcg cgtggtcgag cacgtgcgtt accgacgaca gcgggcgcat
     5221 gaacgacagc ttgaggtgcg gatcgaccca ccacgggaac aggccgccat cgcgcagctg
     5281 ctcggtgtcg gcgacgttgc cattcgcgaa cgtgaacaga tcgagcgagc gcggatgtag
     5341 tccggcgatg ccagtatcgt cgcgctcgac gacacggtgc aggtgatcgt cggccgtgaa
     5401 gtttgcggtg atcgccggcg ccgcgagcgc gatgccaatc gcgagcacga cccacggtgc
     5461 gtagcgtgca ccgagccagc ggaccatcgc gttcacgaac ctagtaatgg cgctcgagaa
     5521 cgtaccagcc gaacgcgcgc agcatcagcc gctggagcga cggatcgagc agcggctcgg
     5581 ccttgcgcca gccagcctcg acgagctcgc gtgcctggtc ggcacacgcc ttgatcgcgc
     5641 cgcagctctg cacgagctcg atcgttgccg cgatcgtcgc gatgtcggtc ggcttgctct
     5701 gtagcgtccg ccacaggtgc tcgcgctggt ccttcggcag gcggctcatc gcgtacgcga
     5761 tcggaagcgt tactacgccg ttcgcgatgt cctcgccgcg ctgcttgagg ttgcccttga
     5821 agccattgag gttgagcacg tcgtccatga tctggaacgc gagcccgacc gcctcgaaga
     5881 actcgccgac cgccgcgatc tgctcgtcgg tgccgccacc ggcgaccgcg cccatgcggg
     5941 ccaggcagcc cgcgggtgcg gcggtcttga ggcgatggca cgccaggatg cgcccggcga
     6001 gctcggtgct gtcaccggtc tcgacgacgc gcggcatcag cgacgccatg ccgccgaggt
     6061 cgatcgcctg gccggcgtga ccggcgcgca tcgcggcgaa gtacaggtcg tagatgcgga
     6121 gcttctgctt gtcgttcagc tcctcggagt gcagctggtt ctgggtcagg aagtacgcag
     6181 ccgtgcccga gttgatcgcg atcggctcgc cgtaggtgac gtgcgtgcac ggcccgccgc
     6241 ggcgtaccgt cgacttgtcc tgcacgtcgt cgacgatcaa cgacccgacg tggagtagct
     6301 cgggcattgc cagccactgc gagaattttc gagagtcgcc gccgacaaca tcacagcacg
     6361 cgagcgcggc atacgagcgc cacgacttgc cgccgcgatc gaccatctcg cggatcggct
     6421 tgatcagcgt ctcggccatc gacggacgat cgacaccgtc catgtaatgc gcgagctctt
     6481 cactcgcgat caggtcgcgc agctcgtcgt gcgtgggctc gagtggcagt gcctgcgcgg
     6541 tcgagcggcg cacttccttg ccgactgccg agaagaagtc gtcgagcgtt tcgatgttct
     6601 cgaagccgct gcgctcgata tacgcgaggc ccgagacctg gcgaccgttc gcggtgccct
     6661 tgacggtgca gcgtccttcc cagaacgccg gcttggagat gacggtgatc agctcctggt
     6721 cggggaacgc ggcttcgatc gtgagatcga tgcccgccga cggcgcagta agccgccagc
     6781 tcgtcgggta gtcgtggaac gtgcgatgcg acgtccacgt cttcaccggc ttgaacgtga
     6841 cgtcgtcgat cgagcgacgc ttcgagtccg cgtcgatccc gatcatccac ttgccgaccg
     6901 tctcgtgcga cttcacatcg atgatctcgt atgcggtgag atcggtgccg tcgtcgagct
     6961 ggatcgcggt ccagttccag ccgaggtcgc gtatgcccgc gccttcgccc tgcgcagcgg
     7021 cgtcggcttt cgtgtcgtcg gcgacgtagc cgccgaattc gtgatcgtac caaccctgcg
     7081 cggcagcgac cggatgcgtt tcgccgccga ccgtgatcgt gccggtcaca gtgcagcgcg
     7141 gaatgaagta atagaacatc tgctcgccgg cgttgccgcg gacgacgccg tcgtcgccgt
     7201 gccggatcgg ctgcttttgc ggcgcgaaca cgagctcgca tgcggtctgc ttgtcgtcgt
     7261 ggacgagctg cacgcgatag ctgccgtcgg cttgcttctc gaaactgccg ctgccgtact
     7321 gcacgtgcag tcggtcgctg gcgatatgca cggggccatc gaacacgcga tcgggtgcgg
     7381 gcacctgacc gcgatcgagg atttctttca tcgcgcggtt gagtcgatcg tccttcgcgc
     7441 cgcggccgtt cgcgaggcgc tgcagaccga gctcgggcgc gcgctgatcg acgcgtgact
     7501 cgccgtgata gctgccgcga tcgagatcgc tgagcgccca cgtatacgag tgcgcgtact
     7561 cgaccgcgcc ggtcttctcg tcgaccgcat tgatgatgcg gaaaaacgcg gcgaacaacc
     7621 cgaacgtgcg gccgtcggca agccgcagat gcgagttgac gtaccaccac tcggtgtcag
     7681 ccgcggggtg cggcaggtcg tgatgcgcga ggtcgatcgt cccttgcgcg ggccaccagc
     7741 ttaatttccc catacggctg gacggcacgc tacacgcgat gcagcgacgg gttcaacgaa
     7801 caatttgctg tgcaaactgt tagtggctgc gcaattcgcg catcgcccgc tttcaatgta
     7861 acggttaagt ttccgccgga tgacactgac gatcgagacc ctcgctgccg agcctcgctg
     7921 ggaactggcg aaggttcagg cgctgcccgg gcgcagcaga ggcgatttgc tcgggtcgtg
     7981 gggcagtaat gtcatgaaac ggtttggttc gaccggcctg caacgtgtgc tgcggcgctt
     8041 accatcgccc ctcgacgcgc taccggcgac gctcggcgcg aaggactggg tgccggtcta
     8101 cgcgcaattg ctcgtgaccg aggcgatcgt cgacgagtac ctcggcggtg acatgcgcgc
     8161 gctctatccg ttgctcgtcg aggatacgcg cgccggcatc ggcagcgtgc agctcgtcgt
     8221 gctcaaggcg atggggcctg tgcgcgcgct caagctcgcg ccgcgaacgt ttcggcacgt
     8281 gcacgagcag ggcagcgtcg acgccgaggt cggcgggcgc agcgcgcgga tgacattctc
     8341 cggctcaccg ctgttcacgc atccgacgtg gcgtctgctc cagctgtttg cgacccacac
     8401 gatgctcgag ctgaccggcc gccgcggttc gtcgtctggc gaaaccatga caaacggctt
     8461 cgtcgcgaac acgaattggt gaaggtcgtt ttcgtgcgac cggaggccgg caagcggctc
     8521 gtttcggcat gaaacagctg tggccaattg tcctcacgct cggaggctgc atcatcagct
     8581 ccgggccctc cgacgatatc gaccaaacgc aggccgacgt cgatcactac aaccagctgt
     8641 cggccgagct cgacgcgcga cgctccgaat ttctcggacg cgacgtgcag gagctcgccg
     8701 cggcgggctc gacgctgtac tggctcgaca cgaccaactt caatccgaag ctgatgcatt
     8761 acgacgggca gacgaagctc gcgtacggct tctcgatcgg cgacggcaac aactacaact
     8821 atcgtgcgtc ggcgagcgtc gtcgtcaccg cagaacccgg ctccgacgcc gtgatctacc
     8881 gcgcgtacga cgctgtcgcc gcaaatcgcc tgctcggcac gacgtcgctg ccgaagccgg
     8941 tcggcgcgaa gtggagcgcg tatgccgtcg acagctcgac ggtctacatc atggacggca
     9001 cgacattgaa gaagtggctg ccgggcggcg acgtgacgac ggtgacaacg ctcgaatccg
     9061 caggcgtcga ggcgggcgaa ttctgggact tcggcgtcgc cagcaacacg atggtgttca
     9121 tcgagagcgg ccgcgtgtgg tcgatggatc tcgcggcgaa ccacgcgacg tggttgatga
     9181 acatgaccga agcaggcggt accgtcgact tccgtcccga cggtgtgttg ttcgataccg
     9241 cgaccggtct gtcattttac gactacgcgc aaaggaagct gatcgacatc cgcgcagcga
     9301 tcgacgcgaa ccccttcaag atcaacaaca cgttcgcgag cgcggcgaag ttcgacacgg
     9361 acttcacgcg ttacaagcag tgggtgctct acatcgggca gagcgggctg ttcgcgtatg
     9421 acttcaaggc cgacaagatc actccgcttt tgctatcgcc gaatcgcgcg gaccttcgcg
     9481 tcgattaccg ctatccggtc gcgctcgata cgggcgtggc ctttgtcacg ggcctcacga
     9541 gcacgagcgg cgccacgggc gccgacggtc ctacgtacac gctggacctc aagccaattc
     9601 tccattgaag aaattgcgca gggccagtca gctcttggag ccgatgaaaa gtttgtgtgt
     9661 cgcctcactg tgtgaggaat tctgactgaa cgccctcgcg aactatcgtt caatagcgcg
     9721 cgcgatgttg atagggtggt gctcgtgt
//
LOCUS       DASUFX010000406         8483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2010922, whole genome shotgun sequence.
ACCESSION   DASUFX010000406 DASUFX010000000
VERSION     DASUFX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010922"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..762
                     /locus_tag="VFV99_16250"
     CDS             79..762
                     /locus_tag="VFV99_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5060921.1"
                     /translation="MRAVVLCADPEQTSREPGSVATLLVELGGRVSVGRFDLGGLDEE
                     ELVAHPPVAVIVEAGDELQRAQKTIQKIRDGGPLVEVPILIAVTVARLPSLDFSIGFD
                     DFVLMPIVPAELYARMRQLDWKSATFGSDEVIKIDDLLIDIAGYEARIGGRRIDLTHQ
                     EFELLRFLAQHRGRVFTRQALLERAWGYAYAGGTRTVDIHVRRVRSKLGTAGELIETV
                     RNVGYKMRG"
     gene            801..2645
                     /locus_tag="VFV99_16255"
     CDS             801..2645
                     /locus_tag="VFV99_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5060922.1"
                     /translation="MAGRASIVGAAETSVGKVRDHNEDAHFIDPEAGILIVCDGMGGH
                     AAGEVASALAVKTIRNRWSSEGVQRAIDSWHKLGTAEARKQLILAIQKGVVDAHDAIV
                     AEAEADRGKAGMGTTLVGAIVVGGDVVFAHCGDSRAYIVRDNIAMQLTEDHTLLARLL
                     AAGVDVDVDGDGMRFKSMLTNALGIGQECKVSTFIVPVADGDRFLLCSDGITEYVKEH
                     EVGEVLSSSASPARACQRLVEMALARGGGDNATALVVRVLEAGETSRPAELLRREERA
                     IHSCPLWAKVNPQQRLRALRIALPRDYALSEKLPAQTLGDRVAWIIVEGDLEQDGHVR
                     GPGSLIYPEALLTDRPLPDKDGLAVAQSDIRVLAIRSDDFRELCEDDPELGEALLAAL
                     AAEIQLRRPHRRTGKIVVNGDGEERSGTDPGLEPTMPGLSTFPRARTPTDRGVSAVAR
                     APTIPPPVDSRAGTEPPARISSPRLSRGSTPPLSSLIQAPTDVEGDLDRALLDLTADK
                     KAVATKQLEPADDEDAEISVEPRKDDTPVPKTEAKRSPNLSEPEISIEALEDEADAAP
                     VEVLKDTSSQKITMTSDASDPEIVVAPLANGDSGPERPRPKRQTDQPD"
     gene            complement(2962..3747)
                     /locus_tag="VFV99_16260"
     CDS             complement(2962..3747)
                     /locus_tag="VFV99_16260"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5060923.1"
                     /translation="MLAGCAGAEAMTLPQRAKPGIVQTTTNEVGLNPGETMAFEVRLG
                     GVLAGEAALAVGALGEFEGKKAVVVKSRANTAGAAALFKRIVDEATTVIDMGSGRPLQ
                     LETLVEQGDKKTTASAKFVGTIADVTYTRSDDPTPHNFKINFGQQAVHDTHSAMAQIR
                     GWRPAAGTTRSVFVVGGRRLWRVDVKFVGEETIGSAVGNRRAIKLEGASFRARPNFTI
                     ETDKPMRTFTVWLSDDADRVPLRVLAKTELGDVTVDLTEYNRQ"
     gene            3831..4787
                     /gene="pdxA"
                     /locus_tag="VFV99_16265"
     CDS             3831..4787
                     /gene="pdxA"
                     /locus_tag="VFV99_16265"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5060924.1"
                     /translation="MEARTKVGITLGDPAGIGPEIVAAALHAAPREWRDRITVYGDRG
                     PLERGAKALGITVPDVALVESGEGDRAVAGTPDRVSGAAQVAYLEAAVAAAARGELAA
                     IVTAPISKTWARRAGFEFLGHTEMLAARLGARDVAMFFVGPKLKVALATVHRPLAEVV
                     TDLTTERVRTVVELVTKSMVRDLGVTAPRIGVVGLNPHAGEDGLLGDEDAEIIAPALE
                     EPLAPATLLGPLVPDAAFRDAVHGRYDALVAMYHDQGLIPVKLVDFEESVNVTLGLPI
                     VRTSPDHGTAYDIAGKGVARTVSMQRALALAIEMVARRAAYV"
     gene            complement(4791..5132)
                     /locus_tag="VFV99_16270"
     CDS             complement(4791..5132)
                     /locus_tag="VFV99_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060925.1"
                     /translation="MTRWMLLMLAGCLVVVEPDPEETTDGHGLPIGGVHACEVRVKDT
                     KAYWTICFQNTGDVSTFETQTMSTCTYNGVDCGVNCSTHELWLCVIRCPGPPLSFDAC
                     NSSYGCYCPPE"
     gene            complement(5268..5864)
                     /locus_tag="VFV99_16275"
     CDS             complement(5268..5864)
                     /locus_tag="VFV99_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060926.1"
                     /translation="MLLAPLLRNILMYGGLVIAAGAVLVLRELYRMRAAERSRLPDAK
                     VVSDERSFRRTFVRWSPALAIAFGIALFYWSYLHRYEYVMVHDGDHGPSVVRRLSPDR
                     IPDVTLAPGQKSPTEEDSFISEGTWVVNLSKRTVRVVTTQYGRGLGFGGEPQPIPPST
                     SAHFVHIDHIGPNDPPPGSVQDDVNLGIAFREWLTWDE"
     gene            complement(5874..6563)
                     /locus_tag="VFV99_16280"
     CDS             complement(5874..6563)
                     /locus_tag="VFV99_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5060927.1"
                     /translation="MPDPKRVLWYLRKVPLLADLGPDAIARMAERVELREARRRDVIY
                     LPGDPGRSLFFVHGGRIKVSKVTRDGKSLTLAYHGPAELFGDACLLDGGPRSEMAEAV
                     ENALLSEIDRVHFEELMHNHPSLGLAMTRLMIVRRRELENKVEALVFRDVSSKLAELL
                     VKLAGEYGVDDARGTLVALKITHQELANLIGSTRETVSLTLSQFKRKRLIQTEGRKVI
                     ISDTEALRALY"
     gene            complement(6737..>8483)
                     /locus_tag="VFV99_16285"
     CDS             complement(6737..>8483)
                     /locus_tag="VFV99_16285"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5060928.1"
                     /translation="PIEAAARVRKKSEETGDPAPTLEAVAEDWVGMARAGRLPPVVGV
                     DPVFDELASLLDEKRLPSMLFVGPRGAGKTALVRRIARGLMDRSRGKGSAKRRLWATS
                     ADRIVAGMIYLGMWQQRCLSIVKELEGGADTLFIDRLADAMAPMSDGASITDLLTPAV
                     LAGELSLFAECDEAELVRARQRNPALLDAMRVVRVPEATPAQVIALLDPLGQRLDPPV
                     VLSHEASRRLVELLAAFRRDTAFPGKAVQFVSYCSLQKQSQLSLAELTTTFGTWSGLP
                     VDLLSPERALDTAAIATELRKGVVGQDAACFVAARVIARLKAGLDDPQRPVGALLFAG
                     PTGVGKTELAKQLARYLFGDGERLVRIDMSEITTPSAIARLITPSPAGTSLADRIRRQ
                     PLSVVLFDEIEKADDAAFDLLLGVLGEGRLTDALGRLIDFRMALIVMTTNLGAADPRP
                     AGFSSDPDAPPDPSNAIKSFFRPELLGRLDSVVAFRPLPPPALERIVDLELDKLRARP
                     GFIARNLRIELTSGARARLAALGHDPKLGARPLRRTIEDLVVAPLAERMAREPTWRDQ
                     TIRIAIRGESGDILI"
BASE COUNT         1453 a         2757 c         2898 g         1375 t
ORIGIN      
        1 cgaggaagaa agggcaggtg acggtaaggg gactcacggc ttgacgcgac cgctcggtaa
       61 cggagatcac tcggagtcgt gcgggctgtc gtcttgtgtg ccgatccgga gcagacctct
      121 cgtgaaccgg ggtcggttgc cacgctcctc gtcgagctgg gcgggcgtgt ctcggttgga
      181 cgctttgatc tcggtggcct cgacgaggaa gagctcgtag cgcacccgcc cgtcgcggtg
      241 atcgtcgagg cgggggacga gcttcagcgc gcacaaaaaa cgatccagaa aatccgtgat
      301 ggcggacctc tcgtggaagt tccgatcttg atcgcggtca cggtcgcgcg cctgccatcg
      361 ctcgattttt cgatcggctt tgacgacttc gtgctgatgc cgatcgtgcc cgcggagctc
      421 tacgcgcgca tgcgccagct cgactggaag agcgcgacgt ttggctccga tgaggtcatc
      481 aagatcgatg acctgttgat cgacatcgcg ggctacgaag cgcgcatcgg tggccgccgc
      541 atcgacctca cgcatcagga gttcgagctg ctgcggtttc tcgctcagca ccgcggccgc
      601 gtgttcacgc gtcaggcgtt gctcgagcgc gcgtggggct acgcgtatgc cggcggcacg
      661 cgcaccgtcg acatccacgt gcggcgggtc aggagcaagc tcggcaccgc cggggagctc
      721 atcgagaccg tccgcaacgt tggctacaag atgcgcgggt aggggcatgt gcccctgcct
      781 tgaccgtgta tcctggttcg gtggcaggtc gggcctcgat cgtaggagcc gccgagacga
      841 gcgtcggcaa agtgcgcgat cacaacgagg atgcgcactt catcgatccc gaggccggca
      901 tcctcatcgt ctgcgacggc atgggtggcc atgcagcggg cgaggtggcg agtgcactcg
      961 cggtcaaaac gattcgtaat cgatggtcga gcgaaggcgt ccagcgcgcg atcgatagct
     1021 ggcacaagct cggcaccgcg gaagcacgca agcagctgat tctcgcgatc cagaagggcg
     1081 tcgtcgacgc gcacgacgcg attgtcgccg aggccgaggc agatcgcggc aaggctggca
     1141 tgggcacgac gctcgtcggc gcgatcgtgg tcggcggtga tgtcgtgttc gcgcattgcg
     1201 gcgactcgcg cgcgtacatc gtgcgcgaca acatcgcgat gcagctcacc gaggatcaca
     1261 cgctgctcgc gcgcctgctc gcggcgggtg tcgacgtcga tgtcgatggc gacggcatgc
     1321 ggttcaagag catgctgacg aacgcgctcg gcatcggcca ggagtgcaaa gtgtcgacgt
     1381 tcatcgtacc ggtcgccgac ggcgatcggt tcctcttgtg cagcgacggc atcaccgagt
     1441 acgtgaagga gcacgaggtc ggcgaggtgc tgtcatcgtc ggcgagcccg gcgcgtgcct
     1501 gccaacgcct cgtcgaaatg gcgctcgcgc gcggcggtgg tgacaacgcg accgcgcttg
     1561 tcgtgcgtgt actcgaagcc ggcgagacgt cgcggccggc ggagctcttg cgccgcgaag
     1621 aacgtgcgat tcactcgtgc ccgctgtggg cgaaggtcaa tccgcagcag cgcctgcgtg
     1681 cgctgcgcat cgccttgccg cgcgactatg cgctgagcga gaagttgccc gcgcagacgc
     1741 tcggcgatcg cgtcgcgtgg atcattgtcg agggcgatct cgagcaagac ggtcacgtgc
     1801 gcggtcccgg ctcgctgatc tatcccgagg cactgctcac cgatcggccg ctgcccgaca
     1861 aggacggcct cgcggtcgcg caaagcgaca tccgcgtgct cgcgatccgc agcgacgatt
     1921 ttcgcgagct gtgcgaggac gatccggagc ttggcgaggc actgctcgcg gcgcttgccg
     1981 ccgagattca gctccgtcgc ccgcatcgcc gaaccggcaa gatcgtcgtg aacggcgatg
     2041 gcgaggagcg cagcggcacc gatccggggc tcgaaccgac gatgcccggc ctctcgacat
     2101 tcccgcgcgc tcgcacgccg acggatcgcg gtgtgtcggc ggtcgcacga gcgccgacca
     2161 tcccgccgcc cgtcgactcg cgtgcgggga ccgagccacc ggcgcgcatc agctcgccgc
     2221 gactgtcgcg cggcagcaca cccccgctgt cgagcctgat ccaggcgccg accgatgtcg
     2281 aaggcgatct cgatcgcgcg ctcctcgatc tcacggccga caagaaggcc gttgccacga
     2341 agcagctcga gcccgccgac gacgaggacg ccgagatcag cgtcgagccg cgcaaggacg
     2401 acacgcccgt cccgaagacc gaagcgaagc ggtcgccgaa cctgagcgag ccagagatct
     2461 cgatcgaggc actcgaagac gaagcggacg ccgcaccggt cgaggtgctc aaggatacgt
     2521 cgtcgcagaa gatcacgatg acgagcgacg catcggatcc cgagattgtc gtcgcgccac
     2581 tcgccaacgg cgactccggc ccggagcgtc cgcgcccgaa gcgccagacc gaccagcccg
     2641 attagtctcg ccgatctgga tccgtgcacg tgcacgtaca cgatctcagc gtcgaaccag
     2701 catcgtttct cccgaaccgg gatcgtgaca ccgaatgtcg tgcccagatc gacgatgcgg
     2761 aacaggtcgc gggcagacaa ccccaaccga gacgccgatc gacgagctgc gatcagcgaa
     2821 ttcccccact cgcccggcac tgctggtcgg tgaccgcact gtcccgagct ccttcagcac
     2881 atgctcgggg caacgcttat cgccgagtgg ggctcggcgg ccgtgcacgt gacacgtcgg
     2941 atcgagtgac cgaaccgtgg gttactggcg gttgtactcg gtgagatcga cggtcacgtc
     3001 gccgagctcg gtcttcgcga gcacgcgcag cggtacgcgg tcggcgtcat ccgacagcca
     3061 cacggtgaac gtgcgcatcg gcttgtcggt ctcgatcgtg aagttcggcc gcgcgcggaa
     3121 cgatgcgcct tcgagcttga tcgcgcggcg attgccgacc gccgagccga tcgtctcttc
     3181 gccgacgaac ttgacgtcga cgcgccacaa ccgccggcca ccgacgacga acaccgagcg
     3241 cgtcgtgccc gccgccggcc gccagccgcg gatctgcgcc atcgccgaat gcgtgtcgtg
     3301 cacggcctgc tggccgaagt tgatcttgaa gttgtgcggc gtggggtcgt cgctgcgcgt
     3361 gtaggtgacg tccgcgatcg tgccgacgaa cttcgccgac gccgtcgtct tcttgtcgcc
     3421 ttgttcgacg agcgtctcga gctgcagcgg gcggcccgag cccatgtcga tcacggtggt
     3481 cgcttcgtcg acgatgcgct tgaacagcgc ggccgcacca gccgtattcg cgcgcgactt
     3541 cacgacgacc gccttcttgc cctcgaactc gccgagcgcg ccgaccgcga gtgccgcctc
     3601 accggcgagc acaccgccga gtcgcacttc gaacgccatc gtctcgccgg gattgaggcc
     3661 gacctcgttc gtggtcgtct ggacgatccc cggctttgca cgctgcggga gcgtcatcgc
     3721 ttcagcgccg gcacacccgg ccagcaccgc cacgagccaa atcgatgtgc gccccaccga
     3781 tatagaatgg acgaaccagc gccgcaggca agcagtgata gggtgtggct gtggaggcgc
     3841 gcacgaaggt cggcatcacg ctcggcgatc cggcggggat tggccccgaa atcgtcgcag
     3901 cggcgcttca cgctgcaccg cgtgaatggc gcgaccgcat caccgtctat ggtgatcgtg
     3961 ggcccttaga acggggcgcg aaggcactgg gcatcacggt tccggacgtg gcgctcgtcg
     4021 aaagcggcga gggcgatcgc gcggtcgccg gtacgccgga tcgcgtgagc ggcgccgcac
     4081 aggtcgcata cctcgaagct gccgtcgcgg cggctgcacg cggcgagctc gctgcgatcg
     4141 tcaccgcgcc aatctcgaag acgtgggcac ggcgcgccgg cttcgagttc ctcggccata
     4201 ccgagatgct tgcagcacgg ctcggcgccc gcgacgtcgc gatgttcttc gtcggcccga
     4261 agctcaaggt cgcgctcgcg accgtgcatc gcccgcttgc cgaagtcgtt accgatctga
     4321 cgaccgagcg cgtgcgcacc gttgtcgagc tcgtgacgaa gtcgatggtg cgtgacctcg
     4381 gtgtgaccgc gccgcggatc ggcgtcgtcg gtctcaaccc gcacgcgggt gaggatggcc
     4441 tgctcggcga cgaggacgcc gagatcatcg cgcccgcgct cgaagagcca ctcgcgccgg
     4501 cgacgctact cggcccgctc gtgcccgacg ctgcgttccg cgatgcggtg cacggccgct
     4561 acgacgcgct cgtcgcgatg tatcacgacc aaggattgat cccggtcaag ctcgtcgact
     4621 tcgaggaatc agtgaacgtc acgctcggcc taccgatcgt gcgcacatct cccgatcacg
     4681 gcactgcgta cgacatcgca ggtaaaggcg tcgcgcgtac agtgtcgatg cagcgcgcgc
     4741 tcgcgctcgc gatcgagatg gtcgcgcgcc gcgccgcgta cgtgtaagcg ctactcggga
     4801 gggcagtagc agccgtacga cgagttgcag gcgtcgaacg acagcggcgg ccccgggcac
     4861 cggatcacgc agagccacag ctcgtgcgtc gagcaattca cgccgcaatc gacgccattg
     4921 tacgtgcacg tgctcatcgt ttgggtctcg aacgtcgaca cgtcgccggt gttctggaag
     4981 cagatggtcc agtacgcctt cgtatctttc acgcggacct cgcacgcgtg aacgccgccg
     5041 atcggtaatc cgtgaccgtc ggttgtctct tctggatccg gttcgacgac gaccaggcaa
     5101 ccggcaagca tcagcaacat ccagcgcgtc atgcagcctc tcgcgaggct ggtcgttgtt
     5161 cacgtcacgt gccgcgtgga gcgcacgcga taaccgtgga ctagatcact ttgcgcggac
     5221 gcgagcgagc gcctgatgct cgacgcgcag gacgccgcgt acgcggatca ctcgtcccag
     5281 gtcaaccact cgcgaaacgc gatgccgagg ttcacgtcgt cttgcaccga gccgggcggc
     5341 ggatcgtttg gcccgatgtg atcgatgtga acgaagtgcg ctgacgtgct cggtggtatc
     5401 ggttgcggtt cgccgccaaa cccgaggccg cgaccgtact gcgtcgtcac gacgcggacc
     5461 gtacgcttgg agaggttcac gacccacgtg ccctcgctga tgaagctgtc ctcttcggtc
     5521 ggcgatttct ggcccggcgc gagcgtgacg tcgggaatgc gatcggggct gagtcggcgc
     5581 acgaccgatg ggccgtgatc gccgtcgtgc accatcacgt attcgtagcg atgcagatac
     5641 gaccagtaga acagcgcgat gccgaatgcg atcgcgagtg ccggcgacca gcgcacgaac
     5701 gtgcgacgga acgagcgctc gtcgctcacg accttcgcgt cgggaaggcg ggaccgctct
     5761 gccgcgcgca tacgatagag ctcgcgcagc acgagtaccg cgccggccgc gatcacgagg
     5821 ccgccgtaca tcaggatgtt ccgcaacagc ggcgcgagga gcatcccgcg aggttagtac
     5881 agagcgcgca gcgcttcggt atccgaaatg atgactttgc ggccttcggt ctggatcagg
     5941 cgcttgcgct tgaactgcga cagcgtgagc gacacggtct cacgcgtgga accgatcagg
     6001 ttcgcgagct cttgatgcgt gatcttgagc gcgacgagcg tgccgcgtgc atcgtcgacg
     6061 ccgtactcgc cggcgagctt gacgagcagc tcggcgagct tggacgagac gtcgcggaac
     6121 acgagcgcct cgactttgtt ctcgagctcg cggcggcgca cgatcatgag gcgcgtcatc
     6181 gcgaggccga gcgatggatg gttgtgcatc agctcttcga agtgaacgcg atcaatttcg
     6241 gagagcagcg cgttctcgac cgcctcggcc atctcgctgc gcggaccgcc atcgagaaga
     6301 catgcatcgc cgaacagctc ggcgggaccg tgatacgcga gcgtgagtga cttgccatcg
     6361 cgcgtgacct tgctgacctt gatgcgaccg ccgtgcacga agaacagcga gcgacccggg
     6421 tcaccgggca aatagatcac gtcgcgcctg cgtgcttcgc gcaactcgac tcgttcagcc
     6481 atgcgcgcga tcgcgtcagg accgaggtcc gcaagaagcg ggaccttgcg caaataccag
     6541 aggactcgct tggggtcagg cacgattcaa ttgtagcagc gctcgcaaca ggatgaaccc
     6601 ttgtcgcgtt gcttactaaa gtggcacgtg cgtgatttcg ccgagttgcg agattggact
     6661 gatctccggc gcacctgaaa tgagccttac gtgggcagcc caacgcgcta atcacatcag
     6721 aattttccct tgatccttag atcaggatgt cgcccgactc gccgcggatc gcgatgcgga
     6781 tcgtctgatc gcgccaggtc ggttcgcgcg ccatgcgttc ggcgagcggc gcgaccacca
     6841 gatcttcgat cgtgcggcgc aatggtcgcg caccgagttt cggatcgtga ccgagtgctg
     6901 cgaggcgcgc gcgagcgccg gacgtgagct cgatgcgaag gttgcgcgcg atgaagccgg
     6961 gacgtgcacg cagcttgtcg agctcgagat cgacgatgcg ctcgagcgcg ggcggtggca
     7021 gtggtcggaa tgcgacgacc gaatcgagac ggccgagcag ctcgggccgg aagaagctct
     7081 tgatcgcgtt gctcggatcg ggcggcgcgt ctggatcgga ggagaagccc gcgggccgcg
     7141 gatcggcggc gccgaggttc gtcgtcatca cgatcagcgc catgcggaag tcgatcaggc
     7201 ggccgagcgc atcggtgaga cgaccttcgc caaggacgcc gagcaagaga tcgaacgcgg
     7261 cgtcgtcggc cttttcgatt tcgtcgaaca gcacgaccga cagcggttgg cggcgaatgc
     7321 gatcggcgag cgacgtgccg gcgggcgagg gtgtgatcag acgcgcgatc gcggacggcg
     7381 tcgtgatctc gctcatgtcg atgcgcacga gccgctcgcc atcgccgaac aagtagcgcg
     7441 cgagctgctt cgcgagctcg gtcttgccga cgccggtcgg gccggcgaac aacagcgcgc
     7501 cgaccgggcg ctggggatcg tcgaggccag ccttgaggcg ggcgatcacg cgtgcggcga
     7561 cgaagcacgc ggcgtcctga ccgacgacgc ctttgcgcag ctcggtcgcg atcgcagcag
     7621 tgtcgagcgc gcgctcgggc gagagtagat cgaccggcag accggaccat gtaccgaacg
     7681 tcgtcgtcag ctcggcgagc gacagctgcg attgcttctg cagcgagcag tagctgacga
     7741 actgcacggc cttgccgggg aacgcggtgt cgcggcggaa cgcggcgagc agctcgacga
     7801 gccggcgcga tgcttcgtgc gacagcacga ccggcggatc gaggcgctga ccgagcggat
     7861 cgagcagcgc gatcacctgc gcgggggttg cttcgggtac gcggacgacg cgcatcgcat
     7921 cgagcagcgc ggggttgcgc tggcgcgcgc gcacgagctc ggcctcgtcg cactccgcga
     7981 acagcgatag ctcgccagcg agcacggcgg gcgtgagcag atcggtgatc gatgcgccgt
     8041 cggacatcgg cgccatcgca tcggcgagcc gatcgatgaa cagcgtgtcg gcgccgccct
     8101 cgagctcctt gacgatcgac aagcagcgct gctgccacat gccgagatag atcatgccgg
     8161 cgacgatgcg atcggcgctg gtcgcccaca gccggcgctt cgcggagcct ttgccgcggc
     8221 tgcgatccat gaggccgcgt gcgatgcggc ggacgagcgc agtcttgccg gcgccacgcg
     8281 ggccgacgaa cagcatgctc ggcaggcgtt tctcgtcgag cagcgacgcg agctcgtcga
     8341 agaccggatc gacgccgacc accggcggga ggcgcccggc gcgcgccatg ccgacccaat
     8401 cctcggcgac cgcttcgagc gtaggggctg ggtcgccggt ctcttctgac ttcttgcgca
     8461 cgcgcgcggc ggcttcgatc ggg
//
LOCUS       DASUFX010000407         6991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1733934, whole genome shotgun sequence.
ACCESSION   DASUFX010000407 DASUFX010000000
VERSION     DASUFX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733934"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFV99_16290"
     CDS             <1..277
                     /locus_tag="VFV99_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060929.1"
                     /translation="YGSWAVYPASFGARHFALTALRVQAELRTMTVDECRDPQRRRTR
                     LARWLDDVVLSGAAEPILDQSAWQMPPAWARHSKKQPPRTLACIETN"
     gene            439..1200
                     /locus_tag="VFV99_16295"
     CDS             439..1200
                     /locus_tag="VFV99_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060930.1"
                     /translation="MTYRNDVDALAARKDALDADIARQTRERDDAARMLDEHRERMRL
                     PVLQNIRVASPCSAKWEDMTGDDRARLCAQCDKHVFNVSELTRVEAEALIVEKAGSLC
                     VRYFQRKDGTILLADCEIGKRGVRRRRVIAAGVVAAMAAGGGIAYAMQEDEAKKEELI
                     AVAGGPMGDPYPYVEPSPVPHAPAPECYAFRDAIFAVDDCISAEMRAHFRGVFVDALA
                     QSRLNDEAQRMQAVETCVNGAETMRGLAKLCVKQP"
     gene            complement(1224..1793)
                     /locus_tag="VFV99_16300"
     CDS             complement(1224..1793)
                     /locus_tag="VFV99_16300"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEX5060931.1"
                     /translation="MLVVTVVIVWIIDGRDRRLGISHGIEHATIALLERLIRFGLGGF
                     ATPDSFVVRIPRESVHLLWRERVRDAAQRAIDQLAAGDRQLAYTPDCSTNWFAERAIL
                     CVCVFAFCAFAYVVSAPPHVGVLAVATAIWFARALGHVLGIALQWWVTVHTRWASVRI
                     VDVEVGEGKDDHAGTYEFRVRLDIVPRPG"
     gene            complement(2103..3245)
                     /locus_tag="VFV99_16305"
     CDS             complement(2103..3245)
                     /locus_tag="VFV99_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060932.1"
                     /translation="MRRILIVCGLTAAVILLVLALRQRDSSARAANARAVGTSPAVAA
                     EPTTQRGPHSLGTNVAVAPSTLDREYGETRAPAEDPLTAYKRINQYPPTSRPLSIHHD
                     LVTPNRRYESPQHAAKDSDVTFWFSGDKGTIVGDDVLHAFLEVRRDGEPIDVRIVSAT
                     ADKLPLTFTKNGERYVNELRLADAQYDTSATVNVRVEFDYGGGMTQEASFPVLYTPKA
                     GVPARFTGTFREAIVNGSLVIYAGIEVKKEGWYLIDANLWAGDDAPVAWTRYKGNLGP
                     SNTEVPLEFFGKVLVDAKSPAPYQVRQLRGARHVENKDPDLENMPFFDGTYTTRTYPL
                     STFSPAEWDSEHKREMIRLLSEQQQTGRHNPASGGDPALAEPHGLP"
     gene            complement(3258..4007)
                     /locus_tag="VFV99_16310"
     CDS             complement(3258..4007)
                     /locus_tag="VFV99_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060933.1"
                     /translation="MSLAPVAAEAATYNLHVSGVCSYNWSDSLGSQDWFTDDNMGFWS
                     GETSVSASVNQTSSMSTAVADLKARLDTYCLNGNLCYIYAWSNGGAVISKVLATYDTA
                     WNIGYIYTSANNEGGSELAGGKWLSEFLAGCSLASAVKPGNFRTAWNHNDTNGKVFYQ
                     VGGDIGPSWALWYVTSGLLPGDDDGIVALHSAGGATCGGSSSGCSVTNMCSAARWSNH
                     SIVGTCAGYSLDHAAIKRKAICNDGGGGNCP"
     gene            4315..4758
                     /locus_tag="VFV99_16315"
     CDS             4315..4758
                     /locus_tag="VFV99_16315"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX5060934.1"
                     /translation="MKEIALNRSGEHQRTFAFVLEPDEDPIAALSERAQAYALASCQI
                     TAVGAFSRVTLGYFDREQKKYLPIPIEEQVEVLSLIGDVAHLDGKRVVHVHCVVGLRD
                     GTTRGGHLLAARVWPTLEVIVNEWPRFLQKRFDPNLGLALIDHGG"
     gene            4846..5205
                     /locus_tag="VFV99_16320"
     CDS             4846..5205
                     /locus_tag="VFV99_16320"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX5060935.1"
                     /translation="MRWTYGLVPIVSGADKFTNLLTDWTQYLSPLATRVVPVKPRTFM
                     RAVGLIEIAAGALVLKRRTSRLGAYVVGGWLAAIAANLVIKRSNYDVAVRDLVMGIGA
                     VSLARMLKIRETRPFAC"
     gene            5208..5762
                     /locus_tag="VFV99_16325"
     CDS             5208..5762
                     /locus_tag="VFV99_16325"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5060936.1"
                     /translation="MSSHAHYSKATLGGHPIHAMIIGLPIALYTTGIVALIVYAVRHD
                     PFWYRASMTLVLAGVATALLAAVFGAIDLFFGIPRATRPRKTGVKHFGLQLLATILFA
                     GAGFMMLGDWVGTPPAPAHLRVAAPLVIGLAGFVAMSVGAWLGWKMVQVEHVGVDITK
                     VEAERANAAHPRASVERQHASPNR"
     gene            complement(6002..6322)
                     /locus_tag="VFV99_16330"
     CDS             complement(6002..6322)
                     /locus_tag="VFV99_16330"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX5060937.1"
                     /translation="MRRPRNPKRWSREVSERSNALDLEPGVFKGSPSEIARSLKRSAD
                     RSKRRKADPFRSALSMLTFYENRAGSKLPASRKRALENAKDELRKLYGRSAPRSRSKA
                     RKRS"
     gene            complement(6329..6505)
                     /locus_tag="VFV99_16335"
     CDS             complement(6329..6505)
                     /locus_tag="VFV99_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060938.1"
                     /translation="MKSVSGDELVIQRDNGSELTLKITERGANMTMPDTGDRVNVQYR
                     FDGMEPVVVKIEAQ"
     gene            6507..>6991
                     /locus_tag="VFV99_16340"
     CDS             6507..>6991
                     /locus_tag="VFV99_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060939.1"
                     /translation="MPSSVCSGPIPCAEDGCADVSLGSVAPGILVVGSVERGVVIGAV
                     ARGVVVSGSVVSGVVVDGSVVFGVDGSVVFGSFTFGIVPDGSVVSGAVVDGSVVFGVD
                     GSVVFGIVDGSVARGVVVDGSVVRVLCCASLRRASNSCRCFSRACVARVCASCARWRA
                     C"
BASE COUNT         1116 a         2233 c         2337 g         1305 t
ORIGIN      
        1 gtacgggagt tgggctgtct atcctgcaag ctttggcgcg cgccacttcg cgctcactgc
       61 gctgcgcgtt caggcagagc tgcgcacgat gactgttgac gagtgccgcg atccgcagcg
      121 gcgtcgcacg cggctcgcgc gttggctcga tgacgtcgtg ctgagcggcg cggccgagcc
      181 gatcctcgat cagtcggcgt ggcagatgcc accggcctgg gcccgtcact cgaagaagca
      241 gccgccgcgc acgctcgcgt gcatcgagac taactagaca cccagctgct acgcgacaaa
      301 gggcgcggag aggcagcctc ttgtaatgag gcgctgacaa tccgtcgaat cctcggcgac
      361 acgtccgtcg tcggccgtag tggttgcgcg gttctacgaa aggccaaaac cgcggcccgc
      421 tcccggtgtc tcactcccat gacctaccgc aacgacgtcg acgcgctggc cgcgcgcaag
      481 gacgctctgg acgccgacat cgcgaggcag acgcgcgaac gcgacgacgc tgcgcgcatg
      541 ctcgacgagc accgcgaacg gatgcggctg ccggtgctgc agaatatccg cgtcgcgtcg
      601 ccgtgctcgg cgaagtggga ggacatgaca ggcgatgatc gcgctcggct ctgcgcgcaa
      661 tgcgacaagc acgtcttcaa cgtgtcggag ctgacgcgcg tggaggcgga ggcgttgatc
      721 gtcgagaagg ccgggtcgct ctgcgtgcgg tacttccagc gcaaggacgg gacgatcctg
      781 ctcgccgatt gcgagatcgg aaagcgcggc gtgcggcggc ggcgcgtgat cgcagcgggc
      841 gtggtagcgg cgatggcggc gggcggcggc attgcgtatg cgatgcagga agacgaagcg
      901 aagaaggaag agctcatcgc ggtcgccggt gggccgatgg gggatccgta tccgtacgtc
      961 gagccgtcgc cggtgccgca tgcgccggcg cccgagtgct acgcgtttcg cgatgctatc
     1021 tttgctgtcg acgactgcat ttcggccgaa atgcgcgcgc acttccgtgg cgtgttcgtc
     1081 gatgcgctcg cgcagtcgag gttgaatgat gaagcgcaac gcatgcaggc ggtcgagacg
     1141 tgcgtgaacg gcgccgaaac gatgcgcggc ctcgcgaagc tctgcgtcaa acaaccttga
     1201 tcgtttgatc gagcgcggcg agctcagccg ggccgcggca cgatgtcgag acggacacga
     1261 aactcgtacg tgcccgcgtg gtcgtctttg ccttcgccaa cctccacgtc gacgatgcgg
     1321 accgacgccc agcgcgtgtg caccgtcacc caccactgca gcgcgatgcc gagaacatgc
     1381 cccaacgcac gagcgaacca gatcgcggtc gcgaccgcga gcacgccaac gtgcggcggt
     1441 gcgctgacga cgtatgcgaa cgcgcagaac gcgaacacgc atacgcagag tatggcgcgc
     1501 tcggcgaacc agttcgtgct gcagtccggt gtgtacgcga gctgcctgtc gcccgccgcg
     1561 agctgatcga tcgcgcgctg cgcggcgtcg cggacgcgct cgcgccatag caggtgaact
     1621 gattcgcgag ggatccgcac gacgaagcta tcgggtgtgg cgaagccgcc gaggccaaac
     1681 cgtatgagtc gctctagcaa cgcgatcgtt gcgtgctcga tgccgtgcga gatgccgagc
     1741 cgtcgatcgc gaccgtcgat gatccacacg atcacgaccg tcacgaccaa cacggcgctc
     1801 agcgcacttg cgatcagaag cagcagaagg ggtagcttca aatattgcgt gaggcttgga
     1861 tacgcgagcg ccccgagcgt ccccagctcg agccagtggt cggtcagcca ctcagtcgct
     1921 gactccctcg ccccttgcca ccacgtcacg attcaccagg ttcgtcgggt gcttgcccgt
     1981 cagcacgagg cgcagtgcgc atagcggctt cggataggtg tggctcgccg gcgaacacgt
     2041 caagggcagc cgcgaagatt tccggccgtc gacgtaaggt cgttgtcgtc gcgcgtcggc
     2101 agctaaggca ggccgtgcgg ttcggcgagc gcggggtcgc caccggaggc ggggttgtgt
     2161 cgaccggtct gctgctgctc gctgagtagc cggatcatct cgcgcttgtg ctcgctgtcc
     2221 cattccgcgg gcgagaacgt gctcaacgga tatgtgcgcg tcgtgtacgt gccgtcgaag
     2281 aacggcatgt tctcgaggtc gggatccttg ttctcgacgt gccgcgcgcc gcgcagctga
     2341 cggacctggt acggcgccgg cgatttggcg tcgacgagca ccttgccgaa gaactcgagt
     2401 ggcacctcgg tgttgctcgg gccgaggttg cccttgtagc gggtccacgc cactggcgca
     2461 tcgtcgcccg cccacaggtt ggcatcgatg aggtaccagc cctccttctt cacctcgatc
     2521 ccggcgtaga tgacgagcga gccattgacg atcgcttcac ggaacgtgcc ggtgaaccgc
     2581 gccgggacgc cagccttcgg cgtgtacagc accgggaagc tcgcctcctg cgtcatcccg
     2641 ccgccgtagt cgaactcgac gcgcacgttg acggttgcac tcgtgtcgta ctgcgcatcc
     2701 gccaggcgga gctcgttgac gtagcgttcg ccgttcttcg tgaacgtcag cggaagcttg
     2761 tccgcggttg ccgacacgat ccgcacgtcg atcggttcgc cgtcgcggcg cacttcgaga
     2821 aatgcgtgga gcacgtcgtc gccgacgatc gtgcccttgt cgccactgaa ccagaacgtc
     2881 acgtcgctgt ccttggcggc gtgctgcggg ctctcgtagc ggcggttcgg cgtgacgagg
     2941 tcgtggtgga tcgacagtgg ccgcgaggtc ggcgggtact gattgatccg tttgtatgcg
     3001 gtgagcggat cctcggctgg cgcgcgcgtc tcgccgtact cgcgatcgag cgtgctcggc
     3061 gcgaccgcga cgttcgtgcc gagcgaatgc ggtccgcgtt gtgtggttgg ctccgcggcc
     3121 acggccggcg acgtcccgac agcgcgcgcg ttggcggcgc gagcactgct gtcgcgttgg
     3181 cgcagcgcga gcacgaggag gatcaccgcg gccgtgagac cgcacacgat caggatgcga
     3241 cgcatggctg acctccgcta tggacagttt ccgccgccgc cgtcgttaca gatcgctttg
     3301 cgtttgatcg cggcgtggtc gaggctgtag ccggcgcatg tgcctacgat cgagtgattc
     3361 gaccatcgcg cggcgctgca catgttcgtg acgctgcatc ccgacgacga gccgccgcat
     3421 gtggcgccgc cggcggagtg gagcgcgacg ataccgtcgt cgtcgccggg caagaggccc
     3481 gaggtcacgt accacagcgc ccacgatgga ccgatgtcgc cgccgacctg atagaagacc
     3541 ttgccgttgg tgtcgttgtg gttccacgcg gtgcggaagt tgcccggctt gacggccgac
     3601 gccaggctgc agcccgcgag gaactccgag agccacttgc cgccggcgag ctcgctaccg
     3661 ccctcgttgt tggcgctcgt atagatgtag ccaatgttcc atgcggtgtc gtaggtcgcg
     3721 aggaccttcg agatcaccgc gccaccgttc gaccacgcat agatgtagca aaggttgccg
     3781 ttgagacagt acgtgtcgag ccgcgccttc agatcggcga cggcagtcga catcgacgac
     3841 gtctgattca cggacgcact gaccgacgtc tcgccggacc agaaacccat attgtcgtcg
     3901 gtgaaccagt cctgtgaccc gagcgagtcg gaccagttgt agctgcacac gccactaacg
     3961 tgcaggttgt aggttgctgc ttccgcggca accggagcga gtgacaaggc aatgacgatt
     4021 gcgaccagcg acctcgaatg catcttcgac ctcccccatg gtgcgttgaa gattcgcaag
     4081 gaggctcacg gcgtgtgcaa tcgcaaaacc acgcgcaacg agccgtaact ggttgagacg
     4141 tggatgtgtg gcgcgatcgg ttgctcagac gatgagccaa gtagctcagg ttctgcgcaa
     4201 cgctgctcaa atgttgggcg acacgaggac caccggtacg ccgagccgac cggtcctcat
     4261 cggcaactgt cgtaggcgcg tgtggtgggc acgtggcttg cgtcgagaga cgatatgaag
     4321 gagatcgcgc tcaaccgaag cggcgaacac caacgaacgt tcgcgttcgt gctcgaacca
     4381 gatgaggatc cgatcgcagc gctgtcggag cgcgcgcaag cgtacgcact cgcgagctgt
     4441 cagatcacag ccgtcggcgc gttctcgcgc gtcacgctcg ggtacttcga ccgcgaacag
     4501 aagaagtatc tgcccattcc gatcgaggag caggtcgaag tgctctcgct gatcggcgat
     4561 gtcgcgcatc tcgacggcaa gcgcgtcgtc cacgtgcatt gcgtcgtcgg gctccgcgac
     4621 ggcacgacgc gcggcggcca tctcctcgca gcgcgggtgt ggccaacgct cgaggtgatc
     4681 gtcaacgagt ggccgcgatt cttgcagaag cgcttcgatc cgaacctcgg gctcgctctg
     4741 atcgatcacg gcggctgatt cgcacgcgtg ttgcacgtgc tgggttacgt cgctcgtcgc
     4801 gacgaggagt ttccgatgtc agctctcgat cgaccgtccg aaatcatgcg gtggacctac
     4861 gggctcgtgc cgatcgtgtc cggcgccgac aagttcacca atcttctgac cgactggacc
     4921 cagtacttga gcccgctcgc cacccgcgtg gtgccggtga aaccccgtac gttcatgcgt
     4981 gcagtcgggc tcatcgagat cgccgcaggc gcgctcgtgc tcaagcgcag gacgtcgcga
     5041 ctcggggcat acgtggttgg cggctggctc gctgcgatcg ccgcgaatct ggtgatcaag
     5101 cgatcgaact acgatgtcgc cgtgcgcgac ctcgtgatgg gaatcggcgc cgtctcgctc
     5161 gcgcgcatgc tgaagattcg cgagacgcgg ccgtttgcgt gctagccatg tcgtcgcacg
     5221 cgcactactc gaaggcgaca ctcggcggcc atccgatcca cgcgatgatc atcgggctgc
     5281 cgatcgcgct ctacacgacc gggatcgtcg cattgatcgt ctacgcggtg agacacgatc
     5341 cgttttggta ccgcgcatcg atgacgctgg tcttggccgg cgtcgcgacc gcgctcctcg
     5401 ccgccgtatt cggcgcgatc gatctgttct tcggcattcc gcgtgcgact cgcccgcgca
     5461 agaccggcgt gaagcacttc gggctgcagc tgcttgcgac catcttgttc gcgggggcgg
     5521 ggttcatgat gctcggggat tgggtcggca cgcctccggc gccggcccac cttcgcgtcg
     5581 cggctccgct cgtgatcggg ctcgccggct ttgtggcgat gtcggtcggc gcctggctcg
     5641 gttggaagat ggtgcaggtc gagcatgtcg gcgtcgacat cacgaaggtc gaagccgaac
     5701 gcgcgaacgc tgcccatccg cgcgcctcgg tcgagcggca gcacgcatca cctaaccgct
     5761 gacgcctcct agtaggtcgc aggtaacgga cgggcgcgac attgatggcg cgtggccggt
     5821 acgttcgatg caacctcgtt actcgcgagg agaccgcaat gttacttccc tggcttctaa
     5881 tgatcctgat cctggcggcg gcgattgccg gcctcatcta cacgacgagg cgtaagtggc
     5941 gcgagcatca ccgcgagccc ggcacgggta ggcgctgatc gcggtcacaa actcgtgccg
     6001 ctcagctccg ctttcgcgcc ttgctacgcg agcgaggtgc cgagcggccg tagagcttgc
     6061 gtagctcgtc cttcgcattc tctagcgcgc gtttccgtga cgccggaagc ttcgagccgg
     6121 cgcggttctc gtagaacgtg agcatcgaga gcgcggagcg aaacggatcc gccttgcggc
     6181 gcttgctgcg atccgccgac cgcttcagag aacgggcaat ctccgacgga ctgcccttga
     6241 acacacccgg ctcgaggtcg agcgcattgc tacgctcgct gacctcgcgt gaccaacgct
     6301 tcgggttgcg gggccttcgc atgtttgcct actgcgcttc gatcttcacc acgaccggct
     6361 ccatgccgtc gaagcggtac tgaacgttca cgcggtcgcc ggtgtcgggc atcgtcatgt
     6421 tggcgccacg ctccgtgatc ttcagcgtca gctcactgcc gttatcgcgc tggatcacga
     6481 gctcgtctcc cgacactgac ttcacgatgc cttcgtcggt ctgcagcgga cccatcccat
     6541 gcgccgagga cggctgcgcg gatgtatcac tgggctcggt cgcacctggc atcctcgtcg
     6601 tcggttcagt cgagcgcggc gtcgtcatcg gcgctgtcgc tcgcggggtt gtggtctcgg
     6661 gttcggtggt ctccggtgtc gtcgtcgatg gctcggttgt ctttggtgtc gacggctccg
     6721 tggtcttcgg ctcgttcacc tttggcatcg tgcccgatgg ctcggtggtc tccggcgccg
     6781 tcgtcgacgg ctccgtggtc ttcggcgtcg acggctcggt cgtcttcggc attgtcgacg
     6841 gctcggtggc ccgcggcgtc gtcgtcgatg gctcggtggt gcgcgttctc tgctgcgcct
     6901 cgttgcgacg agcctcgaat tcttgccgct gtttctcccg cgcctgcgtc gcacgagttt
     6961 gcgcgtcttg cgcccgctgg agagcctgct g
//
LOCUS       DASUFX010000408         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_205602,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000408 DASUFX010000000
VERSION     DASUFX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205602"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2553)
                     /locus_tag="VFV99_16345"
     CDS             complement(<1..2553)
                     /locus_tag="VFV99_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5060940.1"
                     /translation="MSDPVDTSDIRHGAKIGRFVVEGQLGAGAMGVVYAAHDRELDRR
                     VALKVLKGTGEDREALLRLQREGQAMARVTHPNVITVHEAGIQGRLVFLAQELLDGGS
                     LRQWLEEKMFKRSQREILDKFVAAGRGLAAAHAAGLVHRDFKPDNVLLGKDGRVRVSD
                     FGLARSLAGIETVPVKPLDPTGGTVNDIAKSPMATMTMTGAVMGTPLYMSPEQHRGEA
                     ADERSDQFSFSVALYQALYGDQPFPGKTVVALADAVIAGRMQPPPKNAKVTSHLRRVL
                     LRGMATDPAKRYPSMDALLADLTFDPGRKLRRFGVIAAITMLVAGAVVGGYVLRSKTA
                     SYEVPPQRTIAVLGFKNLSGDKSVDWMSSAVSGLIANEIATDEMRVTPAEDVQRVRGE
                     LRLGNEQTFSKETLQKVRDRLASGVVLTGSYLQTGDQLKLTVVIEDLDRRTQQRVEVT
                     KGANELPALAAEAGTKIRAALGIVSVAKASEMLAVMPKNPDAAREYVLGRDALDAFKY
                     GSAKEHLRNAIEKERDFAPGYIALADAHAGLLETDEAKAAARNALDNVKSLGMDQQLL
                     VSGQAYERLGKPANAREQYKKLFAASPKLEYGIALLGVETPDELTATLARVRGIGPDA
                     RIDLIEAQAELDKSNAQRALELAQPAAKTAQDKGAWRVQAEARTIEGQAQLVLGQLGK
                     AAEAFEAARKLYDTAGDDISRVGMMELIAGVSLERGELDDAAAKYELVADKRKGDAAA
                     RAWAASAYATALRGRIANAETQLKKAEQIKPQDPFAITQIDLAAAWIAWAKGDADAAI
                     ARSEQCAARANTDAMHALCLELNGSIRADRADPLARKTLEDGLALAEKINNPQ"
     gene            complement(2570..>2775)
                     /locus_tag="VFV99_16350"
     CDS             complement(2570..>2775)
                     /locus_tag="VFV99_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060941.1"
                     /translation="SQADAIVLANVFVSYRDLYIKGLEAGLGVYNLLDHDNNFYQPYD
                     GGHAPLRDASRQLLLRVGYTVPL"
BASE COUNT          422 a          977 c          857 g          519 t
ORIGIN      
        1 ctgcgggttg ttgatcttct cggcgagcgc gagcccatcc tcgagtgtct tgcgcgccag
       61 cggatccgca cggtcggcgc ggatcgagcc gttcagctcg agacacagcg catgcatcgc
      121 gtccgtgttc gcgcgcgcgg cgcattgctc gctacgcgcg attgcggcgt cggcgtcgcc
      181 tttcgcccac gcgatccagg cggcggcgag atcgatctgc gtgatcgcga atggatcctg
      241 cggtttgatc tgctcggcct tcttcagctg cgtctcggca ttcgcgatcc ggcctcgcaa
      301 tgcggtcgca tatgcactcg ccgcccaggc acgcgccgcc gcatctcctt tgcgcttgtc
      361 ggcgaccagc tcgtacttcg ccgcggcgtc gtcgagctcg ccgcgctcga gactgacgcc
      421 cgcgatgagc tccatcatgc cgacgcgact gatgtcatcg ccggctgtgt cgtagagctt
      481 gcgcgcagcc tcgaacgcct ctgccgcttt gccgagctgg ccgagcacga gctgcgcttg
      541 tccctcgatc gtgcgcgcct ccgcctgcac acgccacgcg cctttgtcct gcgcggtctt
      601 cgctgcaggt tgcgcgagct cgagcgcgcg ctgcgcattc gacttgtcga gctcggcctg
      661 tgcttcgatc agatcgatgc gcgcatccgg gccgatgcca cgaacgcgcg cgagcgtcgc
      721 cgtcagctcg tccggcgttt cgacgccgag gagtgcgatg ccgtactcga gcttcgggct
      781 cgccgcgaac agtttcttgt actgctcgcg agcgttcgcc ggtttgccga gccgctcgta
      841 cgcttgaccg ctcacgagca gctgttgatc catgcccagc gacttcacat tgtcgagggc
      901 gttgcgtgct gccgccttgg cctcatcagt ctccaacagg cccgcgtgcg cgtcagcgag
      961 cgcgatgtag ccgggtgcga aatcgcgctc cttctcgatc gcattacgca ggtgctcctt
     1021 cgccgagccg tacttgaagg cgtcgagtgc gtcacgtccc aagacatatt cgcgcgcagc
     1081 gtcgggattc ttcggcatca ccgcaagcat ctcgctcgcc ttggcgaccg aaacgatgcc
     1141 gagtgcggcg cgaatcttcg tgcctgcttc cgccgcgagg gccggcagct cgttcgcgcc
     1201 tttcgtgacc tcgacgcgct gttgcgtgcg acggtcgagg tcttcgatga cgacggtcaa
     1261 cttcagctga tcgcccgtct ggagatagga accggtgagc acgacaccgc tggcgagtcg
     1321 atcgcgaacc ttctggagcg tctccttgct gaacgtctgt tcgttcccga gtctcagctc
     1381 gccgcgcacc cgctggacgt cctccgccgg cgtcacccgc atctcatcgg tggcaatctc
     1441 gttcgcaatc agaccactca ccgccgacga catccagtcg acgctcttgt cgccgctcag
     1501 attcttgaag ccgagcacgg cgatcgtgcg ctgcggcggc acctcgtagc tcgccgtctt
     1561 cgatcgcagc acgtagccgc caaccaccgc gcccgcgacg agcatcgtga tcgccgcgat
     1621 caccccgaac cgccgcagct tgcggcctgg atcgaacgtc agatccgcaa gcagcgcatc
     1681 catcgacgga tagcgcttcg ccggatcggt cgccatccca cgcagcagca cgcggcgcaa
     1741 atgcgacgtc accttcgcgt tcttcggcgg cggctgcatg cgacccgcga tcaccgcgtc
     1801 ggcgagcgcg accaccgtct tgccggggaa cggctgatcg ccgtacagcg cttgatacag
     1861 cgcgaccgag aagctgaact gatccgaacg ctcgtcggca gcctcgccac gatgctgctc
     1921 gggcgacatg tacagcggcg tgcccatcac cgcgccggtc atcgtcatcg tcgccatcgg
     1981 gctcttcgcg atgtcgttca cggtgccgcc ggtcggatcg agcggcttca ccggcacggt
     2041 ctcgatgccg gcgagcgagc gcgcgagacc gaagtcggac acgcgcacgc ggccatcttt
     2101 gccgagcagc acgttgtcgg gcttgaagtc gcgatgcacg agccccgcgg catgcgcggc
     2161 tgcgagcccg cgtcccgccg cgacgaactt gtcgaggatc tcgcgctggc tgcgcttgaa
     2221 catcttctcc tcgagccact gcctgagcga tccaccgtcg agcagctcct gcgcgaggaa
     2281 tacgaggcgg ccctggatgc cggcttcgtg cacggtgatc acgttcggat gcgtcacgcg
     2341 cgccattgcc tggccttcgc gctggagtcg caacagcgcc tcgcgatcct cgccggtgcc
     2401 cttgagcacc ttgagtgcga cgcgccgatc gagctcgcga tcgtgcgcgg cgtacaccac
     2461 gcccatcgcg cccgcaccga gctgaccctc gaccacgaag cgaccgatct tcgctccgtg
     2521 ccggatgtcc gaggtgtcga cgggatcgct cacgcgcccc gactatacgt tagagcggaa
     2581 cggtgtaccc gacgcgcagc agcaactggc gtgaggcatc tcgcaacggc gcgtgaccgc
     2641 cgtcgtaggg ctgataaaaa ttgttgtcgt gatcgagcaa gttgtacacg ccgaggcccg
     2701 cttcgaggcc ctttatgtac aaatcgcgat acgacacgaa cacgttcgcg agcacgatcg
     2761 catccgcctg cgaga
//
LOCUS       DASUFX010000409         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1177571, whole genome shotgun sequence.
ACCESSION   DASUFX010000409 DASUFX010000000
VERSION     DASUFX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177571"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFV99_16355"
     CDS             complement(<1..828)
                     /locus_tag="VFV99_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060942.1"
                     /translation="MAEVPPPVKDTLLELNAAVREDFTRNRRVLSFSEYLALAFAEPT
                     RQLRSSTQYIVDCFDHYGTEKVTYPFGQVTHFKLFDAPWANGEDRLFGQEPVQNQVYG
                     ILRSFVQDGRPNKLILLHGPNGSAKSTFIRCLGRALEHYSTLDEGALYRFSWIFPAQK
                     STKGGIGCGGGGQSIESMESFAYLPDDQIDARLGDELRDHPLLLVPLAERQKLIGKLL
                     EGKAFQLSDYLRGGALSPRNRAIYDALLASYQGDYVQVLRHIRVERFEISHRYRQGWV
                     "
     gene            complement(869..1465)
                     /locus_tag="VFV99_16360"
     CDS             complement(869..1465)
                     /locus_tag="VFV99_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060943.1"
                     /translation="MSRALVLAILIGSTGCAACGNGSKSPGDQGKVPANASGEEKISV
                     PGAKGAADTGGPGSAATPASAGIGNADDPRFHLKPEEGSITVDKAEGKAGAEATAALK
                     LAPATGYHVATDYPIKLLLEAPAGVKVAKSELSAGGRNKDQGDASTLTEQGLAFAIKA
                     TADKPGSYEIKGVFKFGICEKDSCHPKKQPITITVAAN"
     gene            1526..>2418
                     /locus_tag="VFV99_16365"
     CDS             1526..>2418
                     /locus_tag="VFV99_16365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5060944.1"
                     /translation="MRRAQQLGRYHLLDRIAFGGMAEIYRAKTFDGNGTPHLVAVKRV
                     LAHLAEDDDFIQMLVDEAKIASVLRHQSIARVYEFARAHGEYFIAMEHVDGKDMRTVL
                     ERCRAKKKPIPPEHAAYICAEVGAALHAAHSAVDSRGRPLRIIHRDVSPSNIICSYSG
                     EVKLCDFGIAKATLSRVTTKTGVIKGKVKYMSPEQALGRKLDHRSDVFSLGSCLYEML
                     TRIPPFTATNEMDLLIMVRDAKYRPVSELTPGIPHELEAIADKCLTRSRANRYQTAAE
                     AEGDLRAFLRKFMPNYSRSHLG"
BASE COUNT          417 a          799 c          782 g          420 t
ORIGIN      
        1 gacccagccc tggcgatagc gatgcgagat ctcgaagcgc tcgacgcgga tgtgccgcag
       61 cacctggacg taatcgccct gatagctcgc gagcagcgcg tcgtagatcg cgcgattgcg
      121 cggtgacagc gcaccgccac gcagatagtc gctgagctgg aacgccttgc cctcgagcag
      181 cttgccgatg agcttctggc gctccgcgag cggcacgagc agcagcggat gatcgcgaag
      241 ctcgtcgccg aggcgcgcgt cgatctgatc gtcgggcaga tacgcgaacg actccatcga
      301 ttcgatcgac tggccgccgc cgccacagcc gatgccgccc ttcgtgctct tctgcgccgg
      361 gaagatccag ctgaagcgat agagcgcgcc ttcgtcgagc gtcgagtagt gctcgagcgc
      421 gcggccgagg cagcggatga acgtcgactt cgcggagccg ttcgggccgt ggagcaggat
      481 cagcttgttc ggccggccgt cctgcacgaa cgagcgcagg atcccgtaga cctggttctg
      541 gaccggctcc tggccgaaca agcggtcctc gccgttggcc cacggagcat cgaacaactt
      601 gaagtgcgtc acctggccaa acgggtacgt gaccttctcg gtcccgtagt gatcgaagca
      661 gtcgacgatg tactgcgtcg acgagcggag ctggcgtgtc ggctcggcga acgcgagcgc
      721 gaggtactcg gagaacgaca gcacgcggcg attgcgcgtg aagtcttcac gcacagcggc
      781 gttcaattcg agcagcgtgt cttttaccgg cggcggaact tcagccacgg ctccccccga
      841 tcgcttcgct catcgaaacg accttacttt agttagcggc gacggtgatc gtgatcggct
      901 gcttcttcgg atggcagctg tccttctcgc aaatcccgaa cttgaagacg cccttgatct
      961 cgtagctgcc gggcttgtcg gcggtggcct tgatcgcgaa cgcgagcccc tgctcggtca
     1021 gcgtcgaggc atcgccttga tccttgttgc ggccacctgc cgacagctcg gacttcgcga
     1081 ccttcacgcc ggcgggagcc tcgaggagaa gcttgatcgg atagtcggtc gcgacgtgat
     1141 agccggtcgc gggagcgagc ttgagcgcgg cggtcgcctc agcgcctgct ttgccctctg
     1201 ccttgtcgac ggtgatcgat ccctcctccg gcttgaggtg gaagcgcgga tcatccgcgt
     1261 tgccgatgcc cgcgctagca ggtgttgcag cggatccggg tccacccgtg tcggccgcgc
     1321 ctttcgcgcc gggaaccgag atcttttctt cgccggaggc gttcgccggc accttgccct
     1381 gatcacccgg ggacttggag ccgtttccac acgcagcgca accagtgctg ccgatcagaa
     1441 tcgctaggac gagagcgcga gacatgcccg ctagcatagc ggatctcggc ggttacgcca
     1501 tggcgtggta ctctggacgt tgccggtgag aagggctcag cagctcggcc gttaccactt
     1561 gctcgatcgc atcgcgttcg gtggcatggc cgagatctat cgcgccaaga cgttcgacgg
     1621 gaacggcacg ccgcacctcg tcgcggtcaa gcgcgtgctc gctcacctcg ccgaggacga
     1681 cgacttcatc cagatgctcg tcgacgaagc gaagatcgcg agcgtgctcc gtcaccagag
     1741 catcgcgcgc gtctacgagt tcgcgcgcgc gcacggcgag tacttcatcg cgatggagca
     1801 tgtcgacggt aaggacatgc gcaccgtgct cgagcgctgc cgcgcgaaga agaagccgat
     1861 cccgcccgaa cacgctgcgt atatctgtgc cgaggtcggc gccgcgctgc acgccgcgca
     1921 ctccgcggtc gactcgcgcg gccggccgct gcgcatcatc caccgcgacg tttcgccgtc
     1981 gaacatcatc tgcagctatt cgggcgaggt gaagctgtgc gacttcggca tcgcgaaggc
     2041 gacgctgtcg cgcgtgacga cgaagaccgg cgtcatcaag ggcaaggtca agtacatgag
     2101 ccccgagcag gcgctcggcc gcaagctcga tcaccgcagc gacgtgttct cgctcggcag
     2161 ctgcctctac gagatgctga cgcggatccc accgttcacc gcgacgaacg agatggatct
     2221 gctgatcatg gtgcgcgacg cgaagtaccg cccggtcagc gagctcacgc ccggcatccc
     2281 gcacgagctc gaggcgatcg ccgacaagtg cctcacgcgc agccgcgcga accgctacca
     2341 gaccgccgcc gaggccgagg gtgacctgcg cgcgttcttg cgcaagttca tgccgaacta
     2401 ctcgcgcagc cacctcgg
//
LOCUS       DASUFX010000410         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_483992,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000410 DASUFX010000000
VERSION     DASUFX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483992"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFV99_16370"
     CDS             complement(<1..230)
                     /locus_tag="VFV99_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060945.1"
                     /translation="MIKVHVLDDGTKRFQVYGRARVRDKSVKVYVGTYASQRDAEAAD
                     EEHRVTQRGIKAGELPPAVDSKRTLGDALDAWL"
     gene            complement(315..1115)
                     /locus_tag="VFV99_16375"
     CDS             complement(315..1115)
                     /locus_tag="VFV99_16375"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX5060946.1"
                     /translation="MITEIAFRILEAWLLCAIVQAVLWMIAERTKNAGIVDVGWAQSF
                     TAVVLLFALQPLAPKAGWLPIAALVIAWSTRLTCYLVSRGSATGAEEGRYVELRRRWA
                     LHASQRFFGFFQLQGALTALLSTAFVVPFVVAPWDNGWLRILGFLITTIGIVGETVAD
                     AQLAHWKRDPANVGKVFTFGLWAYSRHPNYFFEWVTWLGFAIYGLAFPTPWAFLPLLG
                     QAIILISILKVTGIPPTENHALRSKGEAYREYQRRVSKFIPLPPKTGT"
     gene            complement(1112..1993)
                     /locus_tag="VFV99_16380"
     CDS             complement(1112..1993)
                     /locus_tag="VFV99_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060947.1"
                     /translation="MRLWALLLLTVACGRDAKDAPRKHYEDAHVAVVPAVDAASPAPA
                     VSSDSYKDLAAALAAVIPADARVIGFGELHSRTDRAQVQSALSRFTTNGLPGMADKLS
                     DLIVETWIVDPKCGQAAKTATAKVEITVRRPQETKSEIAVLAETARAAKIQPHAMKLS
                     CDDYDRVAPSGKDVIPDVMLGLITKELGRIAEEAIKHRDKEPGHRPWIALYGGSLHND
                     RFPAEGVEDWSYAARVDQLTGGKFIEVDLIVPELAEADPTMQKQPWFPLVKDSDPNVR
                     VWKRGERSFVVLFPRSK"
     gene            2025..2885
                     /locus_tag="VFV99_16385"
     CDS             2025..2885
                     /locus_tag="VFV99_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5060948.1"
                     /translation="MGPSASELRTIPLFHGFGDDELAEIADLFKQVDAGDKPLFDVNE
                     PATTLYLLTKGEVVLDRPGDDVFRLRPPALIGELGALTGLARSTRATVTSGAVVWALP
                     AKKLQAYLGDHQELGVRFLVNLLGTVADKVHRDQRRMADMRANLISTQKELKRLRELV
                     LESVETPISAPVHDTLDKLITHNRRVNYRVEPPPALAAHCRFEKSTSPIADISRTHVT
                     VQLANDPPATGSWVSGVMHLAGTEIPISGRVHRTQGHKMTVELDLMIDEYAAALEGYL
                     TRAQLLDILC"
     gene            complement(2882..>3407)
                     /locus_tag="VFV99_16390"
     CDS             complement(2882..>3407)
                     /locus_tag="VFV99_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060949.1"
                     /translation="VGAGCDFVERNERGSAWAIGLDIPVVGYGQHEGAIVPGFGLEAA
                     LHLHPMFDFLVHGDIVVMPRGDDERALHQTVLAGGRIDFSHHPQPGDVKGVFATLGAG
                     YDRVAAPSSAAVQSGPIVEGSVAWGLQGSDGAAWLRLHGRFGVGPDNSDLRAVFLSLG
                     MEIRLDRNRWRDRN"
BASE COUNT          606 a         1163 c         1089 g          549 t
ORIGIN      
        1 agccacgcat cgagcgcgtc gcccaacgtg cgcttggagt cgaccgcagg tggcaactcg
       61 cccgccttga tcccgcgctg cgtgacgcga tgctcttcat ccgcggcctc ggcgtcgcgc
      121 tgcgaagcgt acgtgccgac gtagaccttg acgctcttgt cgcgaacgcg agcgcgtccg
      181 taaacctgaa agcgttttgt cccatcgtcc aacacgtgca ccttgatcat ggcaaggagc
      241 ctacgatggg cctgcgacag ggcggggtag aaacggggta gaaatcaaca atcgcgagat
      301 cggaccgcgg tcggtcaggt cccggttttt ggaggcagcg ggatgaactt cgagacccgc
      361 cgctggtact cgcggtacgc ctcacccttg ctccggaggg catgattctc cgtcggcgga
      421 atgcccgtta ccttgaggat cgagatcagg atgatcgcct ggccgagcaa cggcaggaac
      481 gcccacggcg tcgggaacgc gaggccgtag atcgcgaagc cgagccacgt cacccactcg
      541 aagaagtagt ttggatggcg cgagtacgcc cacaggccga acgtgaagac cttgccgacg
      601 ttcgctggat cgcgtttcca gtgggcaagc tgcgcatcgg cgacggtctc gccgacgatg
      661 ccgatcgtgg tgatgaggaa gccgaggatc cgtagccagc cgttgtccca cggcgccacg
      721 acgaacggca cgacgaacgc ggtcgacagc agtgcggtca gcgcaccctg cagctggaag
      781 aaaccgaaga agcgctgcga tgcgtgcagc gcccagcgcc gccgcaattc gacgtaccgg
      841 ccttcctcgg cacccgtcgc cgagccgcgc gacacgagat aacaggtgag ccgcgtgctc
      901 cacgcgatca cgagcgccgc gatcgggagc caaccggcct tcggtgcaag cggctgcaga
      961 gcgaacaaga gcacgacggc ggtgaacgat tgcgcccacc cgacgtcgac gatgccggcg
     1021 ttcttcgtgc gttccgcgat catccacagc acggcctgga cgatcgcgca cagcagccac
     1081 gcctcgagga tgcggaacgc gatctcggta atcatttgct ccgtgggaag agaacgacga
     1141 acgagcgctc gccgcgcttc cacaccctca cgttgggatc gctgtccttg acgagcggaa
     1201 accacggttg cttctgcatc gtcggatctg cttccgcgag ctctggcacg atgagatcga
     1261 cctcgatgaa cttgccgccg gtcagctgat cgacgcgcgc cgcgtagctc cagtcttcga
     1321 cgccttccgc tggaaagcga tcgttgtgca gcgagccgcc gtacagcgcg atccacgggc
     1381 gatggccggg ttccttgtcg cggtgcttga tcgcttcctc ggcaatccgg cccagctcct
     1441 tcgtgatcag gccgagcatg acgtcgggaa tcacgtcctt gccgctgggc gcgacgcggt
     1501 cgtagtcgtc gcacgacagc ttcatcgcgt gcggctggat cttcgcggca cgcgcagtct
     1561 cggcgagcac ggcgatttcc gacttcgtct cctgcgggcg gcggaccgtg atctcgactt
     1621 tcgccgtcgc ggtcttcgcg gcttgcccgc atttgggatc gacgatccag gtctcgacga
     1681 tcaagtcgct gagcttgtcg gccatgcccg gcaggccgtt cgtcgtgaac cgcgatagcg
     1741 cggactgcac ctgcgcgcga tcggtgcgac tatgcagctc gccaaagcca atcacgcgcg
     1801 catcggccgg aatgaccgcg gcgagcgctg cggccaggtc cttgtacgaa tcactggata
     1861 ctgcaggtgc cggtgaggcc gcatcgaccg ctgggacaac cgcgacatgc gcgtcttcgt
     1921 agtgcttacg aggcgcatct ttggcgtctc tcccgcaggc gacggtcagc agaagcaggg
     1981 cccagagcct caccctgcaa cgataccgcg atatgttccg cgccatgggt ccgagtgcca
     2041 gcgagctgcg taccatcccg ctgtttcacg gcttcggcga cgacgagcta gccgagatcg
     2101 ccgacctgtt caaacaggtc gacgccggcg acaagccgtt gttcgacgtc aacgaaccag
     2161 cgacgacgct ctaccttctc accaagggcg aggtcgtgct cgaccgtccc ggtgacgacg
     2221 tgttccgact gcggccgccc gcgttgatcg gcgagctcgg tgcgctgacg ggcctcgcac
     2281 gctcgacgcg cgccacggtg acgtctggcg cagtggtctg ggcgctgccc gccaagaagc
     2341 tccaggcata tctcggcgat caccaggagc tcggcgttcg cttcctggtc aatctgctcg
     2401 gcacggtcgc cgacaaggtc caccgtgacc agcgccgcat ggccgacatg cgagcgaatt
     2461 tgatctcgac gcagaaggag ctcaagcgct tgcgcgagct cgtgctcgag tccgtcgaga
     2521 ccccgatctc cgcgccggtg cacgacacgc tcgacaagct catcacgcac aaccggcgcg
     2581 tgaactaccg cgtcgagccg ccgcccgcgc tcgccgcgca ctgccgcttc gagaagagca
     2641 cctcgccgat cgccgacatc tcgcgcacgc acgtcaccgt gcagctcgcg aacgatccgc
     2701 ctgcgaccgg cagctgggtc tcgggcgtca tgcacctcgc gggcaccgaa attccgatct
     2761 ccggccgcgt ccatcgcacg cagggccaca agatgacggt cgagctcgat ctcatgatcg
     2821 acgagtacgc ggccgcactc gagggctacc tgacgcgcgc gcagctgctc gacatcctct
     2881 gctaattgcg gtcgcgccag cgattgcggt cgagccggat ctccatccct aacgacagga
     2941 acaccgcgcg cagatccgag ttgtctgggc cgacgccgaa gcggccatgc agccgcagcc
     3001 acgccgcgcc atccgagccc tgcagacccc acgcgacgga gccttcgacg atgggccccg
     3061 actgcaccgc cgcggacgac ggagccgcga cacggtcgta gcccgcgccg agcgtcgcga
     3121 agacgccttt gacatcgccc ggctgcgggt ggtgcgagaa gtcgatgcgg ccgccggcga
     3181 gcacggtctg gtgtaacgcg cgctcgtcat cgccgcgcgg catcacgacg atgtctccgt
     3241 gcacgaggaa gtcgaacatc ggatggagat ggagcgcggc ttcgaggcca aagcccggca
     3301 cgatcgcgcc ctcgtgttgc ccgtaaccga cgaccggaat atcgagcccg atcgcccagg
     3361 ccgagccgcg ctcgttgcgc tcgacgaagt cgcagccggc gccgacg
//
LOCUS       DASUFX010000411         9029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_484483,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000411 DASUFX010000000
VERSION     DASUFX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484483"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VFV99_16395"
     CDS             complement(<1..1543)
                     /locus_tag="VFV99_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060950.1"
                     /translation="MRHLVCVLLLVLSSTAEAQSKAKPKAKPAAAKSAKQATKQVAKQ
                     VDAATAKQLGAAYRAYDDNDLAGAKKHLAKIDDGKVIALDYLLWLRGMVALRSGDAAA
                     AKTAFEKLAKVSPSSFGAQVPWRLADCAWELGDRAGAAKAYAKLINSDSAGDIGDVGT
                     AKFRIAEATPTAQGYRVVVLDHPGHPLAARAEQRMLELGGAPLDANDHLTRAKQLSTA
                     HLWDQAIAELALIPQTGIPEALANQRDYWTGETLFSMRRRYGDAAKLLLAVYAKLDSP
                     QAMFHGARALSRADQDDDAIVWYGKVVATYPRTPWAEEAAFLIGWLDFNRGKYKEAIK
                     PLETSLAKYPRSKWVDDATWFLGMAHYFLGEWDAARERLAALAKYSGSLEGGKGTYWL
                     ARIDERLKKNDAAIAGYRTTVEKHPFSWYSLLARSRLAALGVKVGPFGTDNPKPQGPA
                     LAATVDAKLANDELILRSDELTAAGLGADAGDELARNEKAFLKRNDRAAAFAMLLDRY
                     RKAGNF"
     gene            1575..2741
                     /locus_tag="VFV99_16400"
     CDS             1575..2741
                     /locus_tag="VFV99_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5060951.1"
                     /translation="MAYAALDYYAIDDLFTHEERMVRDTVRELVTTRVMPSIGKHWSA
                     GTFPHELVPVFGEMGLLGPSLTGYGCPGITPTAYGLICQELERGDSGIRSFCSVQGSL
                     VMYPIWAFGSEEQKQKWLPEMAAGRVIGCFGLTEPDFGSNPNGMLTTATRTQGGYRIT
                     GTKRWITNGSVAHVALVWAKLDGKVHGFLVPTKSKGFTARDIHGKWSLRASVTSELIL
                     EDVEVGEDALLPGVSGMRGPLSCLSQARFGICYGAVGGAMACYDEALSYAKDRVQFSR
                     PIAGYQLVQRKLVDMVQEITKAQLLCWRMGRLKEAGTLTPAQISLAKRNCVAMARDIA
                     RDARDILGANGVTDEYQCGRHMLNLESVYTYEGTHDIHTLVVGQDVTGLAAFDG"
     gene            2744..3550
                     /locus_tag="VFV99_16405"
     CDS             2744..3550
                     /locus_tag="VFV99_16405"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX5060952.1"
                     /translation="MTNPYRGAAQSRRPDVLVCSGLDPSGGAGLIADVRVVSELGGRP
                     AGVVTALTVQNTTGVVDVQPCDPDLVGHQLAFLLTDVEVKAVKIGMLGSTAIAKAIAN
                     ALQLTHAPVVWDPILAPTRGVVSLIDADFDEALAALRPHLTIITPNKHELFQLTSRPT
                     GDHVMAEAAARVLANKLDCAVLVKGGHFPGDDDAVDVLVTTSERHVLRGKRVPGGEDV
                     HGTGCALSSAIATYLGQGRDLGEACTLAKQFVANLIEHPSHPGRGASAVL"
     gene            3547..3921
                     /locus_tag="VFV99_16410"
     CDS             3547..3921
                     /locus_tag="VFV99_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060953.1"
                     /translation="MKKTKRKAKTSSRGGGNSRGGGNSRGGGGDSPDAVLEAIDKFVA
                     ARKAEVARMTDKAIKAKRHNPEQHRALQLARAELAEALKLRLRVAKVVLDVSQRLRMI
                     ENSARFAIATAFADYQQRTSRS"
     gene            3923..4906
                     /locus_tag="VFV99_16415"
     CDS             3923..4906
                     /locus_tag="VFV99_16415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX5060954.1"
                     /translation="MKIVVTGATGFVGTPLVAALRERGDDVIALVRDVERAKQHLRPD
                     ATVDAPKPERTPGAKQPGALHLVLAELETPGVWCDRLDGADAIIHLAGESIAGKRWDA
                     RQKQLIRDSRVETTRTIVEAIGKTTTKPKALVTASGVDYYAFAPTKHEFDDDEITESD
                     PAADTFLGRLCRDWENEARAAESFGVRVVSLRCGLVLGKHGGAIAQMRRPFDLFAGGR
                     MGSGRQWMSWVHLDDVIAGYIAAATDERFRGPLNMVTDSTRNADFAKTLGKALGKPTW
                     LPVPAFAIKAAVGSEFAESLLEGRRVMPAKLRELGFAWKYPTLEGALAASV"
     gene            complement(4909..5706)
                     /locus_tag="VFV99_16420"
     CDS             complement(4909..5706)
                     /locus_tag="VFV99_16420"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5060955.1"
                     /translation="MERISDSPARWLDLRPVGIVALAAAMVWSTWIVADTYHSIKVKP
                     EQRNIRITGSAKKRIVSDLIEWSATLEATAPDRTTAYKQLRGYTDRTIEFLKGQGIKA
                     EEIQPQSTSFEEVIEVTEELKVLPGVKEPVKVEKKTPKGFKTTQVILVRSTDVGKVEK
                     ASREVTTLLEEGVSVTSDAPEYFYTRLGELKIEMLAAAGKDARARADNILKNAGGAKV
                     GRLVAADMGIININPANSTETSEEGNNDHSSLEKDIITIVHATFQLD"
     gene            complement(5769..7010)
                     /locus_tag="VFV99_16425"
     CDS             complement(5769..7010)
                     /locus_tag="VFV99_16425"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5060956.1"
                     /translation="MRSPWWQGLDLRVLRHVRGVLRRRGLELTLVERRPSEPAAFAAL
                     STEDASGPGLAVIAPDLSSPEVGLIEHMLGEAALDVAAALRERDGGGNGEPARRSAYA
                     GIIGAAPSMIELYSLLDRVSPSDSTILIQGENGTGKELVARAIHERSPRKENRFVVTN
                     CSAFNDNLLDSELFGHKRGAFTGAVADKAGLFETADMGTFFLDEIGDMSPTLQVKVLR
                     VLQEGTFNRVGDTETRKVDVRIIAATNRDLAAMVAAGQFREDLFYRINVINVTLPALR
                     ERRDDVPILIDYFLGRHRRNGRPKRLTPDCQAQMLGYPWPGNVRELENEIERLVVLAG
                     DSPAIDASLLSPRIRQFTPAEEEPTYDTGSLPAAVEALERKMIGAAIRRHGGNKTRAA
                     EELKVSRRNLIRLVQKYQLEK"
     gene            complement(7269..7934)
                     /locus_tag="VFV99_16430"
     CDS             complement(7269..7934)
                     /locus_tag="VFV99_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5060957.1"
                     /translation="MSERREVVIGQTVLGRPIEAICFTPPSYAKSRPPALLFGAIHGD
                     EPVSQLMLERLADELVERPPGRETWIVPVVNVDGLLAGTRNNANDIDLNRNFASSSWG
                     TERRPGYNPGNAAEDQPETQALVALIDQVKAERLIAVHATYRMINWDGRGEELAKKMA
                     ELCGYPAERDMGYPCPGSFGTKYGVERGLEVITVESPYLIADERGWTECRSALRWCVD
                     LPD"
     gene            complement(7931..>9029)
                     /locus_tag="VFV99_16435"
     CDS             complement(7931..>9029)
                     /locus_tag="VFV99_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060958.1"
                     /translation="PAGYQAGGVSPHAAANAATMFASGPPPGAQPSPPAPQYGGGPAP
                     GYGAPSPGYGNPAPGYGAPAPAYQPPPSSAGMGIHSPQQATPAPLPAVHQPYLGAQTG
                     GVAHAGRPVEPWKDPLKLWMFVWGAIALAAFATPVMTDPMAFQWDAIIHGEGKMKIPG
                     LVWASVGLLSIVFAAIPMSTLPRGALAAVLGIAGIFVPLAVTAFPPWQILVPMIGSLA
                     LVSGLLVRHEYVESLLARALVTVGVVCTLAPYLIPEHGQIPLVLIFKALLSGGNHMEI
                     VIVALGQIVLVVLCLLAWMPGPATAGAKVFAWAVILYPILGFLLTLLGNGHIGDVISK
                     QPGAMVAWAPGVAYSVLVGYGLATVIGKQLE"
BASE COUNT         1517 a         3013 c         2959 g         1540 t
ORIGIN      
        1 tgaagttgcc cgccttgcga tagcgatcga gcagcatcgc gaacgcagcc gcgcgatcgt
       61 tgcgcttgag aaatgccttc tcgttgcgcg ccagctcgtc gcccgcatcc gccccgaggc
      121 cggccgcggt gagctcgtca ctgcgcagga tcagctcgtc gttcgcaagc ttcgcgtcga
      181 cagtggcggc aagcgccggg ccttgcggct tcgggttgtc ggtgccgaac ggaccgacct
      241 tcaccccgag cgccgcgagt cggctgcgcg cgagcagcga gtaccacgag aacggatgct
      301 tctcgacggt ggtgcggtag ccggcgatcg ctgcgtcgtt cttcttgagc cgctcgtcga
      361 tgcgcgcgag ccagtacgtg cctttgccgc cctcgagcga gccgctgtac ttcgcaagcg
      421 cggcgaggcg ctcacgcgcg gcgtcccact cgccgaggaa gtagtgcgcc atcccgagga
      481 accacgtcgc gtcgtcgacc cacttgctgc gcggatactt cgcgagcgac gtctcgagcg
      541 gcttgatcgc ttccttgtac ttgccgcgat tgaaatcgag ccagccgatg aggaacgctg
      601 cttcctcggc ccacggcgtg cgcggatacg tcgcgacgac cttgccgtac cagacgatcg
      661 catcgtcatc ctgatcggcg cgcgacagcg cacgtgctcc gtggaacatc gcttggggcg
      721 aatcgagctt cgcgtagacg gcgagcaaca gcttcgcggc atcgccgtac cgccgtcgca
      781 tactgaacag cgtctcgccg gtccagtagt cgcgctggtt cgcgagtgct tcgggaatgc
      841 cggtctgcgg gatcagcgcg agctcggcga tcgcttgatc ccacaggtgc gcggtcgaca
      901 gttgcttcgc gcgcgtcaga tgatcgtttg cgtcgagcgg cgcgccaccg agctcgagca
      961 tgcgttgctc cgcgcgggcg gcgagcggat ggcccggatg gtcgaggacg accacgcgat
     1021 agccctgcgc ggtcggggtc gcttctgcga tccgaaactt tgcggtgccg acgtcgccga
     1081 tgtcacccgc gctgtcgctg ttgatcagct tcgcgtacgc cttcgccgcg ccggcgcgat
     1141 cgcccagctc ccacgcacaa tccgcgagtc gccacggcac ctgcgcgcca aagctcgacg
     1201 gcgagacctt cgcgagcttc tcgaacgcgg tcttggccgc cgccgcatcg ccgctgcgca
     1261 gtgcgaccat gccgcgtagc cacaacaggt agtcgagcgc gatgaccttg ccgtcatcga
     1321 tcttcgcaag gtgcttcttg gcccctgcca agtcgttgtc atcgtaggcg cgataggcgg
     1381 cacccagctg cttggcagtc gcggcgtcga cctgtttggc gacctgcttc gtggcctgtt
     1441 ttgcggattt ggccgccgca ggtttcgcct tcggcttggc ctttgattgc gcctctgccg
     1501 tcgacgacag cacgaggagc agcacgcaca ccaaatgccg cacggtcctt cgagtatgtc
     1561 gtataacgcc cgcgatggca tacgcggcgc tcgactacta cgcgatcgac gatctcttca
     1621 cgcatgaaga gcggatggtg cgtgacacag ttcgcgagct ggtaacaacg cgtgtcatgc
     1681 cgagcatcgg caagcactgg tcggccggca cgttcccgca cgagctggtg ccggtgtttg
     1741 gtgaaatggg attgctcggt ccgtcgttga ccgggtacgg ctgtcccggc atcacgccga
     1801 ccgcgtacgg attgatctgt caggagctcg agcgcggcga ctcgggaatt cgctcgtttt
     1861 gctccgtgca gggctcgctc gtgatgtatc cgatctgggc gtttggctcg gaggagcaga
     1921 agcaaaagtg gctgcccgag atggcggcgg gccgcgtgat cggctgcttc ggattgaccg
     1981 agcccgattt cggctcgaac ccgaacggca tgctgacgac ggccacgcgc acgcagggcg
     2041 gctaccggat caccggtacg aagcgctgga tcacgaacgg ctcggtcgcg cacgtcgcgc
     2101 tcgtgtgggc caagctcgat ggcaaggtgc acggcttcct cgtgccgacg aagtcgaagg
     2161 gcttcacggc gcgtgacatc cacggcaagt ggtcgctgcg cgcgagcgtg acgagcgagc
     2221 tgatcctcga ggacgtcgag gtcggcgagg acgcgctgtt gccgggtgtg agcggcatgc
     2281 gcggcccgct gtcgtgtctg tcacaagcgc ggttcggtat ctgctacggc gcagtcggtg
     2341 gggcgatggc ctgctacgac gaagcgctgt cctacgcgaa agatcgcgtg cagttcagcc
     2401 ggccgatcgc gggttatcag ctcgtgcagc gcaagctcgt cgacatggtc caggagatca
     2461 cgaaagcgca gctcttgtgc tggcgcatgg gccgcttgaa ggaagcgggc acactgacgc
     2521 cggcgcagat ttcgctcgcg aagcgcaact gcgtcgcgat ggcgcgtgac atcgcccgcg
     2581 acgcgcgcga catcctcggc gcgaacggtg tcaccgacga gtatcagtgc ggccgccaca
     2641 tgctgaacct cgagagtgtc tacacgtacg agggcacgca cgacatccac acgctcgtcg
     2701 tcgggcaaga cgtgacgggc ctcgccgcgt tcgacggcta gccatgacga acccgtaccg
     2761 cggggcggcg cagagtcgca gaccagatgt gctggtgtgc agcggcctcg atccatctgg
     2821 cggcgccgga ctgatcgccg atgttcgcgt cgtctccgag ctcggcggcc ggccggccgg
     2881 tgtcgtcacc gcgttgacgg tgcagaacac gacgggcgtc gtggacgtgc agccatgcga
     2941 tccggatctc gtcggccatc agctcgcgtt cctgctcacg gatgtcgagg tgaaggccgt
     3001 gaagatcggc atgctcggct cgactgcaat tgcgaaggcg atcgcgaacg cgctgcagct
     3061 cacccacgcg ccggtcgtgt gggatccaat cctcgcgccg acgcgcggcg tggtgtcgct
     3121 catcgacgcc gacttcgacg aggcgctcgc cgcgctgcgg ccacacctga cgatcatcac
     3181 gccgaacaaa cacgagctct tccagctcac ctcgcgcccc accggcgatc acgtcatggc
     3241 agaggcagcc gcacgcgtgc tcgcgaacaa gctcgactgt gcggtgctcg taaaaggcgg
     3301 ccacttccct ggcgacgacg atgcggtcga cgtgctcgtc acgaccagcg agcgccacgt
     3361 gttgcgcggc aagcgggttc caggtggcga ggacgtgcac ggcaccggct gcgcgctgtc
     3421 atctgcgatc gcgacgtacc tcgggcaagg ccgcgacctc ggcgaagcgt gtacgctcgc
     3481 caaacagttc gttgcgaact tgatcgaaca tccgtctcac cccggacgag gagcatcggc
     3541 ggtcctatga agaagacgaa acgcaaagcg aagaccagct cacgtggtgg cggcaactcg
     3601 cgtggtggcg gcaattcgcg tggtggtggc ggcgactcgc cggacgccgt gctcgaggcg
     3661 atcgacaagt tcgtcgcggc gcgcaaggcc gaggtcgcgc gcatgacgga caaagcgatc
     3721 aaggcgaagc gtcacaatcc ggaacagcac cgcgcgctgc agctcgcacg tgcggagctc
     3781 gccgaagcgc tcaagctccg gctgcgcgtc gcgaaggtcg tgctagacgt ctcgcagcgc
     3841 ctgcggatga tcgagaactc ggcacgattc gcgatcgcga cggcgttcgc cgattaccag
     3901 cagcgcacat cgcggagctg acatgaagat cgtggtgacg ggggcgacgg ggtttgtcgg
     3961 cacaccgctc gttgcagcgc tgcgcgagcg tggcgacgac gtgatcgcgc tcgtgcgcga
     4021 cgtcgagcgg gccaagcagc acctgcggcc ggatgcaacg gtcgacgcac cgaagccgga
     4081 gcgtacgcca ggcgcgaagc aaccgggtgc gctgcacctc gtgctcgcgg agctcgagac
     4141 gccgggcgtg tggtgcgacc ggctcgacgg cgccgacgcg atcattcatc tcgcgggcga
     4201 atcgatcgcg ggcaagcgct gggatgcgcg ccagaagcag ttgatccgcg acagtcgggt
     4261 cgagaccacg cgcacgatcg tcgaggcgat cggcaagacg acgaccaagc cgaaggcgct
     4321 cgtgaccgcc tcgggtgtcg attactacgc gttcgcgcca accaagcacg agttcgacga
     4381 cgacgagatc accgaatccg atccggcggc agacacgttc ctcggccggc tgtgccgcga
     4441 ctgggagaac gaagcgcgtg cagccgagtc gttcggcgtg cgcgtggtga gcctgcgttg
     4501 cgggctcgtg ctcggcaagc acggcggtgc gatcgcacag atgcgccggc cgttcgatct
     4561 attcgccggc ggtcgcatgg gtagcggccg gcagtggatg tcgtgggtcc acctcgacga
     4621 cgtgatcgct ggctacattg cagcggcaac cgacgagcgg ttccgcgggc cgctcaacat
     4681 ggtcaccgac tcgacgcgca atgccgattt cgcgaagaca ctcggcaagg cgctcggcaa
     4741 gccgacgtgg cttccggtgc ccgccttcgc gatcaaggcg gcggtcggca gcgagtttgc
     4801 cgaatcgcta ctcgaaggcc gccgcgtcat gccggcgaag ctgcgcgagc tcggctttgc
     4861 gtggaagtac ccgacgctcg aaggcgcgct cgccgcgtcg gtctgacgtc agtcgagctg
     4921 gaacgtcgcg tggacgatcg tgatgatgtc cttctccagg ctcgagtgat cgttgttgcc
     4981 ctcttcggat gtctccgtcg agttcgccgg gttgatgttg atgatgccca tgtcggcggc
     5041 gaccaggcgg ccgaccttcg caccgcccgc gttcttgagg atgttgtcgg cgcgcgcgcg
     5101 tgcatccttg cccgccgcag cgagcatctc gatcttgagc tcaccgagtc gcgtatagaa
     5161 gtactcgggc gcgtcgctcg tgaccgacac gccttcctcg agcagcgtcg tcacctcgcg
     5221 tgacgccttc tcgaccttcc cgacgtcggt cgaccgcacc aggatcacct gcgtggtctt
     5281 gaaccccttc ggcgtcttct tctcgacctt caccggctcc ttcacgccgg gcaggacctt
     5341 cagctcctcg gtcacctcga tgacctcttc gaagctggtc gattgcggct ggatctcctc
     5401 ggccttgatg ccctgcccct tgagaaactc gatcgtgcga tcggtgtagc cgcgcagctg
     5461 cttgtacgcg gtcgtgcgat caggcgcggt cgcttcgagc gtcgccgacc actcgatcaa
     5521 atccgacacg atccgtttct tcgcggagcc ggtgatccgg atgttccgct gctcgggctt
     5581 cactttaatg ctgtgatagg tgtccgcgac gatccacgtc gaccacacca tcgcagccgc
     5641 cagcgcgacg atgccgaccg gccgtaggtc gagccacctc gctggcgagt cactgatccg
     5701 ttccatgggc gtttccctcc ttgacggagg ccgaacgcct gacccggcgg ccttattcgc
     5761 gagtcggctt atttttcgag ctgatacttt tgaacgagcc ggatcagatt gcgccgagac
     5821 accttcagct cctcggcagc gcgggtcttg ttgccgccgt ggcgccggat cgccgcaccg
     5881 atcatcttgc gctcgagtgc ctcgacggcc gcgggcagcg atccggtgtc gtacgtaggc
     5941 tcttcctcgg cgggggtgaa ctggcgaatg cgcggcgata gcagtgacgc gtcgatcgcc
     6001 ggcgagtcac cagccagcac gacgagccgc tcgatctcgt tctcgagctc gcgcacgttg
     6061 ccgggccacg gatacccgag catctgcgcc tggcaatccg gcgtcagccg cttcgggcgg
     6121 ccgttgcggc gatgccggcc caagaagtag tcgatcaaga tcggcacgtc gtcgcggcgc
     6181 tcgcgcaacg cgggcagcgt gacgttgatg acgttgatcc ggtagaacaa gtcttcgcgg
     6241 aactgaccgg ctgcgaccat cgcagccaga tcgcgatttg tcgccgcgat gatgcgaacg
     6301 tcgaccttgc gtgtctcggt gtcaccgacg cgattgaacg tgccctcttg cagcacgcgc
     6361 agcaccttca cctgcagcgt cggcgacatg tcaccgatct cgtcgaggaa gaacgtgccc
     6421 atatcggcgg tctcgaacaa ccccgccttg tcggcgacag ctccggtaaa cgcaccgcgc
     6481 ttgtgaccaa acagctccga gtcgagcagg ttgtcgttga acgccgagca gttcgtgacg
     6541 acgaaccggt tctctttgcg cggcgagcgc tcgtgaatgg cgcgcgcgac cagctccttg
     6601 ccggtgccgt tctcgccctg gatcaggatc gtcgagtccg acggcgagac gcgatcgagc
     6661 agcgagtaca gctcgatcat cgacggcgcc gcaccgatga tccctgcgta cgccgaacgc
     6721 cgcgccggtt cgccgttgcc gccaccatcg cgctcgcgca acgccgcggc gacgtcgagc
     6781 gccgcttcgc cgagcatgtg ctcgatcagc cctacctcgg gcgacgacaa gtccggcgcg
     6841 atcaccgcca accccggccc actcgcatcc tccgtcgaga gcgccgcaaa cgcggccggc
     6901 tcgctcggtc gccgctcgac gagcgtcagc tccaatcctc gacggcgaag gacgcctcgc
     6961 acgtgccgga gcacgcgaag atcgagcccc tgccaccaag gagaccgcat ttgtccgccc
     7021 agtgtgacaa cgagtcacag tcttgccaag tcccgacgac gatcggccgc tcaccgcccc
     7081 gctacctcca ggtctcacgc ctgtgtccgg ttcgcttacc gaacagtggt caataccgac
     7141 acgggtgtta gtttgatgcg cgcgtcaagc tacggataac agccgcgtcc cgttcgtcgt
     7201 cggtcgggta attcagtcgg gtatgttcgt cagcgcgaaa ttcgatccgc gaatttcagt
     7261 cggcgaattc agtccggcag atcgacgcac caccgcagcg cactgcggca ttcggtccag
     7321 ccgcgttcgt ccgcgatcag gtagggcgac tcgaccgtga tgacttcgag cccgcgctcg
     7381 acgccgtact tcgtgccgaa cgagcccgga cacgggtacc ccatgtcgcg ctccgcggga
     7441 tagccgcaca gctcggccat cttcttcgcg agctcctcgc cgcggccgtc ccagttgatc
     7501 attcgatacg tcgcgtgcac cgcgatgagc cgctccgcct ttacctgatc gatcagcgcg
     7561 acgagcgcct gcgtctccgg ttgatcctcg gccgcattgc cggggttgta accaggccgc
     7621 cgctcggtgc cccagctcga cgacgcgaag ttgcgattga gatcgatatc gttcgcattg
     7681 ttgcgggtgc ccgcgagcaa gccgtcgacg ttcacgacgg gcacgatcca ggtctcgcga
     7741 ccgggcgggc gctcgactaa ttcgtcggcg agccgctcga gcatcagttg actgaccggc
     7801 tcgtcgccgt ggatcgcgcc gaacaagagc gcaggcgggc gcgacttcgc gtacgacggc
     7861 ggcgtgaagc aaatggcctc gatcggtcga ccgaggaccg tctggccaat caccacttca
     7921 cgtcgttcgc tcactccagt tgcttaccga tgacggtcgc gaggccgtag ccaaccaaca
     7981 cgctgtacgc aacgcccgga gcccacgcga ccattgcacc cggctgcttc gaaatcacat
     8041 cgccgatgtg accgttgccg agcaacgtca acaggaaccc gaggatcgga tagagaatca
     8101 ccgcccacgc gaacaccttc gcgcccgccg tcgccggtcc cggcatccac gcgagcaggc
     8161 agagcacgac caacacgatc tggccgagtg caacgatcac gatctccatg tgattgccgc
     8221 cgctcaggag cgccttgaag atcaacacga gcggaatctg cccgtgctca gggatcaggt
     8281 acggcgcgag cgtacacacc acaccgacgg tgacgagcgc gcgtgcgagc agcgactcga
     8341 catactcgtg acgcacgagc aagcccgaca cgagcgcgag cgagccgatc atcggcacga
     8401 ggatttgcca cggcgggaac gcggtcaccg cgagcggcac gaagatgccg gcgatgccga
     8461 gcaccgcggc gagcgcaccg cgcggcagcg tcgacatcgg gatcgcggcg aacacgatcg
     8521 agagcagacc gacgctggcc cacacgaggc caggaatctt catcttgcct tcgccgtgga
     8581 tgatggcgtc ccactggaac gccatcggat ccgtcatcac cggcgtggcg aacgcggcga
     8641 gcgcgatcgc gccccacacg aacatccaca gcttgagtgg atccttccac ggctcgaccg
     8701 gacgaccggc atgggcgacg ccacccgtct gcgcgccgag gtacggctga tgtaccgccg
     8761 gcagcggcgc gggcgttgct tgctgcggcg agtgaatgcc catgcccgcc gacgagggcg
     8821 gcggctgata cgcgggcgcg ggggcgccat agcccggtgc tggattgccg tagcccggcg
     8881 acggcgcacc gtagccaggc gcgggaccac cgccgtactg cggcgcgggc ggactcggtt
     8941 gcgcaccggg cggcgggcca ctcgcaaaca tcgttgcggc attggccgcg gcatgcggcg
     9001 acacgccacc agcttgatac ccagcgggc
//
LOCUS       DASUFX010000412        17933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_206490,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000412 DASUFX010000000
VERSION     DASUFX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17933
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206490"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1063
                     /locus_tag="VFV99_16440"
     CDS             239..1063
                     /locus_tag="VFV99_16440"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5060959.1"
                     /translation="MRGLVLGLFVIAACSSDSTPPGKIYGGDRPAELKTPATLTEGKQ
                     YPLLVVLHGFGASGFVQTAFFGVGGLPGEDKALLIAPDGTENSTGQQFWNADPACCDF
                     EHHNPDDVAYIGGLIDDISADWPVDKKQVFILGHSNGGYMAYRMACERADVIAAIASL
                     AGNASSTSANCNPSQKVNVVHLHGTADDTVPYSGATPSVDQWATKNGCGTTRSETTTY
                     DFDSAVEGAETHVNPQADCPANGDVELWTMEGSTHIPVMNATFAPTIFDWFTAHKR"
     gene            1116..1358
                     /locus_tag="VFV99_16445"
     CDS             1116..1358
                     /locus_tag="VFV99_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060960.1"
                     /translation="MTPIARVVSSPLAQLVSLVAAAALLVMIVVTQLRPQPSVVVMMP
                     PAAPVVGQPAAAPVPMPVVFPYCYSWAGSWYGYCRD"
     gene            complement(1372..1824)
                     /locus_tag="VFV99_16450"
     CDS             complement(1372..1824)
                     /locus_tag="VFV99_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060961.1"
                     /translation="MRIASLVCAALVVTACTKSDEAEMERKFVVKEITALEKALADGS
                     ESGVTVECAALRASLPRLDATTVARIERLCDGEGAKLFLRKAIADVNARNAQYPNTRE
                     INCMQLFAKTAFESFGRRPEPELEKLVTEYATLCPGEAAKIRNPPPIH"
     gene            complement(1844..3781)
                     /gene="mutL"
                     /locus_tag="VFV99_16455"
     CDS             complement(1844..3781)
                     /gene="mutL"
                     /locus_tag="VFV99_16455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX5060962.1"
                     /translation="METVIAVLPPTVIDQIAAGEVIERPSSVVKELIDNAIDAGARTI
                     SVETLAGGRNLVRVVDDGRGMNPKDAVLAFERHATSKLRGLDDLWGLATLGFRGEALP
                     AIASVSRLTLTTRQAGDLAATRVHIEGGKLVEVSEIGAPVGTCVEVVDLLYNVPARLK
                     FMKGEATEASHITELVARVAMVHHELHLRLKHNGRVALDTPPDRDGFARVQSLLGARI
                     ASRMVPVSGQEGGTRVIAYLGAPELAQTTARGVQLFVGKRPVRDRGLLHALAMGYGEL
                     VPRGRYPTAIVMVDVPAGAVDINVHPQKHEVRFADAGAVCAAVRHVVQAGIEKAPWRD
                     EIGGAGPVMMTAIASVAPPKLPFDAPATAMAQTYAAQLREAREREWTRDRAQAQLGFD
                     DGPRQWAQQIKDRVRSARAAEAELAARAAYALNRVEREQERDAAPWDEAQIAEGSTPA
                     TAPGFFASLKYLGQLDLTYLVCEGDGELVLIDQHVAHERVELARLKAGQGEREVPTQR
                     MLFPTTIELKPELLDVAAKMTDVLAQVGYEAESFGKGTLAVKAVPGGIRHGDPTHLLR
                     KLLTDWADEGAPSEAERLERVLAEIACHSVVRAGDRLSPSEAEALFRSMDGADFSSHG
                     VHGRPVMLRMSLADIARRFGR"
     gene            3851..4852
                     /locus_tag="VFV99_16460"
     CDS             3851..4852
                     /locus_tag="VFV99_16460"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5060963.1"
                     /translation="MSSTKPGRVVAVTGACTYLGLELLRRLEEDTRYSRVLALDIRPP
                     AVSGKKIEYIKLDLTQPTVDSELATILERHRVDTFVHGAFLSHPTHAAEWAHELEDVG
                     TMHVLNACAGVEPRRLVMISTTLVYGAHPKNPNFLTEEAELRGHRDSRFVNDKVRAER
                     QVQRFASEHPNVEVCILRFAPIIGPTVSNMYTRFFRRPVAPVMMGHDPLMQFVHEQDA
                     AFALVKAVDSHATGPFNIVGKGVLPYTTVLALLGRVPVPMPQLVARQVSKILWATQLV
                     GSPPSFLDFLLYLCVADGSKAKSALGFSPRLSIKRTILDFLGVAPEDGATDVARAQA"
     gene            4857..5966
                     /locus_tag="VFV99_16465"
     CDS             4857..5966
                     /locus_tag="VFV99_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5060964.1"
                     /translation="MTDEPDDESTDDEWYGDADPRVPDATAWPDRDLGFLGSDYGGKA
                     AVDSDITGRARTADIDEEAELRELERRVEQQRTPAFPIDLRTRLPLEGLWRRWRALAM
                     WGRSDVVDDFGRDPRATARWEWLFEFLYSKWFRVQAIGVENIPAHGRALLVANHAGTL
                     PYDSAMVMHAVRRDHPSRRDVRPLVEDTVFHLPFLGPIMNRIGGVRADPANAERLLEK
                     DELVAVFPEGEKGMGKLWKDRYRLQRFGRGGFVKLALRTRAPIIPVAVVGAEEAAPML
                     GKVTWFAKNIGIPWIPVTPTFPWFGPAGLLPLPSKWFVQFGEPIDLAAQNGPDAAEDR
                     LLVNKLADQIRTQIQSMIDGLLGKRRSPLLAAFLG"
     gene            5966..6355
                     /locus_tag="VFV99_16470"
     CDS             5966..6355
                     /locus_tag="VFV99_16470"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5060965.1"
                     /translation="MLLDRDLELLYARALISIARAEGTIDSDEGARLGERIAERSGLE
                     LADLLLERSLQPEELADALMRTGAPFRGASVRADEVGRALVEDALYVALAKGHVTPEE
                     GHRMWRYAIALGLSADEFRALTAHWLP"
     gene            6430..6969
                     /locus_tag="VFV99_16475"
     CDS             6430..6969
                     /locus_tag="VFV99_16475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5060966.1"
                     /translation="MPAAYESELERLLEAARELLGAGDDALLVEANATVNQALQLTPD
                     CVEAWLLKCQVASATGDDLAALAAVEMALHRAPHRAEALYWRGAVLGDLGRHREALRA
                     IEAALRVLGDSDHWLLEDLFYEKVMILDALGLHDAAVAACEAGLDRCPGSPLLRAALA
                     PAERARVRSSLKVLRGGAG"
     gene            7046..8233
                     /locus_tag="VFV99_16480"
     CDS             7046..8233
                     /locus_tag="VFV99_16480"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5060967.1"
                     /translation="MKLTTSLVLVLGLAVFGCSKKKEEGTAQKSEPAMTPKTTEKPAE
                     PPKPTPLTGTALADQYKKCVDMVNAGKFDDFKKECIDDSFTMHTGAGMPEMKGADALV
                     AHFNEMKTAFPDMKLQPQLIMVSGRNILAVNLHTGTHTGTMKTPMGDVPATNKKVGML
                     FFHRLAINDANKATEEWAMMDPMTMMGQLGLNPKTAPPVRPAMDKGMEGAPIVVVTAD
                     DAKEKANMDAVKKANDAFIANKPADAMAVMTDDAVESDQATEKDHKGKKDIEAGFKTF
                     RNGWSDVKLSDVQMWAAGDYVVQSFKFEGKNDKDIGKMKKTGKTVALDAVEVMHFKDG
                     KIDQLWRFMNGMDFAQQMGMMPAPGAAPAGAKPGEPKAGEAPKAGEAPKAGEPMKKEP
                     EKK"
     gene            8318..9223
                     /locus_tag="VFV99_16485"
     CDS             8318..9223
                     /locus_tag="VFV99_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060968.1"
                     /translation="MFRRVAGLAACGMVAVAGVWWIGGAIAGDAPSIGPPPADAHVLT
                     EAARAGRHVRIDGPRGAIHVWIPQSYRPETGATIVYVHGYYDDADTAYVGHRLPEQFA
                     MSALNAMFIVPEAPYMQKVPVNYPNLSELIRLVEDKTGVSRGMALTAAVGHSGAYRTI
                     NSWLDEPLLDQIALVDAMYANEEVIEAWLRASPVHRMITVGEDTLQWNEQLLRDMPDM
                     FVIDRVPPTFDTWPAEAKTAKAVYVRAQYYHMPLVTDGIVLPGVLRMLPVELLADEPW
                     RQPLGAMPPLPDAAVDAVSGDAVSD"
     gene            9240..10760
                     /locus_tag="VFV99_16490"
     CDS             9240..10760
                     /locus_tag="VFV99_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5060969.1"
                     /translation="MRTRIAALVAAGALLAFAYVMPGERRRFMQKVITWDAEPSEAIA
                     LQPGMGLGMTPASRVRVILIDGLAETTAETLPNWSAQCKRGIALTVDVGFPTVSLPVE
                     VTLWTGLMQQQTGIVFRSDRPLVPPLQHSIAAQVPGSRAVAEDHGYIVRSLGFADTKP
                     VAVDPAHPGKDADPEAWRAAWLPAARDAVTSDARLAFVHVLRVDTWGHKKGRDSDEYR
                     KAASEADAIVGELVALAPDARWFLLSDHGHLPTGGHGGEERFVRQVQHCIIGPELAPA
                     HGGPVHLVDISRAIADSVGVTPDRASQGRPLTVAMHAPLENDDAVPPLALPIGAIAIF
                     ILALGLAGSSFGVRRWWMAPWWFVLAGVALVIWRGQPTMSTPMVYARDSFFDIFDFDR
                     RLMFRSWLLVLPIAAITTWYGLARKTLWRVLVAQLALPFAALAACITACAGWGALFGG
                     EIAPVVPRYTAYTSALMLITAHGAAVVGLTVLARTAHSASGQRRPAETSRTAPSSE"
     gene            complement(10645..13212)
                     /locus_tag="VFV99_16495"
     CDS             complement(10645..13212)
                     /locus_tag="VFV99_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5060970.1"
                     /translation="MSDDSSSRRGRSPRRPEPQPSPGTSSTPSAPPDARGSYPSQPPD
                     GRGSTPSQPPDGRGSTPSQPPLRRASPLLPDQRRSSNSSWPATRGSTPSGRLSPPGRL
                     SPPGRASPPDRGSTPSDRGLAAPVHGSSGRISSDRISSSGALAPDAAPAPAPDLRTWW
                     IIVAHTLGAAALGSVDSARLGSLGIALAVVPLFAAMGLITGVIVALMQRLVVDRPWWL
                     AALVLTAPALVVFVVVAISLFRGAYAQTLPLSGAAPFLLPLVLWLLSAGAVALGRRVL
                     RATDLTTRGIVVLALAGMMGTVIWVERHVLGTGYPTAHLGATLGLIVLIGVAVRVTRR
                     GGLPYLIAAVVMGLALGGAIIACLGGLKSPVDRQLLATYGDQSRDIVNLWRHVLDFDR
                     DGSSKVLGGGDCDDTDASRHPGAVDKPGDGIDQDCDGKDAPQPTSVAPKPAPKREDLA
                     SWRATPAIADVLAKTRGMHVIVISVDALRADLLAPDAPDRADFPAITKLLSESVWFTH
                     AFAPASGTDVSLSTFLTGRFDPFQPVAMTLPEAMQASGRHTFAALPAEVLRYAGEVLL
                     GRGIDKVATVHTDWDTADVGDHVSAAATTSEGIKALDALGDRPGFLWLHYFDVHEHHQ
                     IDVPKRLREAVHAGATPKVTKYRALLRAIDDEVGRLVDELAKRNLTDKTIIMFASDHG
                     ESLGEDPRLLDTHGKVTYAPLVRIPVAFKIPGVTPGQRTDQISLVDLAPTLLALAGVT
                     PSDMPLDGIDLVPSLLDAPAALRHPPRSVAIHEELQWSVVEWPYQLIVRPSDNIEELY
                     DLEKDPRQLDNIVTKQPDVVTRLKGQYATFPQVVVDRTPNGRSERERLARLRPPRGQ"
     gene            complement(13344..13898)
                     /locus_tag="VFV99_16500"
     CDS             complement(13344..13898)
                     /locus_tag="VFV99_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5060971.1"
                     /translation="MSNAEHKPSILIVDDDEVYRTRLARAFVDRGYDTRTAQDYDSAV
                     AAATTDSPEFAVVDLKMPGKSGLELVKALREIDPATKTVVLTGYGSIATAIDAVRLGA
                     TYYLSKPADADDIVAAFARGEAPPLEPPADHDYKAPSLARAEWEHINRVLSDAGGNIS
                     EAARRLGIHRRSLQRKLQKYPPNK"
     gene            complement(13924..15270)
                     /locus_tag="VFV99_16505"
     CDS             complement(13924..15270)
                     /locus_tag="VFV99_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5060972.1"
                     /translation="MAPPPTIPERNWVNIQWLARLRWAEVAGQAVTVIAAQFLLGGSL
                     PIAPLLAVIGIGLISNVAIEMYFFGDRRRGRVEPKPVHDWQLALVMMFDIASLTALLY
                     LTGGPHNPFSFLYLVQIALATVVVRARWTWMLVGLSFIGFGILLVAHQPLRIPESSHA
                     YGAWVALGVASAFVVHFLLRITAAVAERERELTEARGLAARQERLASLATMAAGAAHE
                     LSTPLGTVALAAKELERALASTNAELASDARLIREQVGRCRSILEQMAQGAGTVGESV
                     TSCTVAELIAETLVGIRDAPKVLNEITDDVARHALRLPPRAVSQALRSLVTNAQDASP
                     PTSAVVVTAHFDDDTLAVGIRDRGQGIPSDVKERIGEPFFTTKAPGRGMGLGLFLARA
                     VIEGVGGTMQIDSAPGGGTEVQVRLPSDVGARSSVAAHEPVRTSESTTGRVVSKPA"
     gene            complement(15339..15881)
                     /locus_tag="VFV99_16510"
                     /pseudo
     CDS             complement(15339..15881)
                     /locus_tag="VFV99_16510"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(15894..16163)
                     /locus_tag="VFV99_16515"
     CDS             complement(15894..16163)
                     /locus_tag="VFV99_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060973.1"
                     /translation="MKALVLLCGALLMACQADTTKLNEKIDKLDKKLDALLAQGGGRG
                     AQPGAQRPSRPEPDRAKTYAVPIDGDSFEGPADAKVTIVEASDYA"
     gene            complement(16248..17069)
                     /locus_tag="VFV99_16520"
     CDS             complement(16248..17069)
                     /locus_tag="VFV99_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5060974.1"
                     /translation="MKALVLLFGAALMACQADTTKLNEKVDKIDKKLDALLAQGGRPG
                     AAQQPRAPRPGPDPAKTYAVPIDNDPFDGPANAKITVVKAYDYACPFCERVRSTMDDL
                     HKKYGNDIRVVYKQLVVHPNTAMSGALAFCAAAKQGKAPQYDNLIWEKSFKARQFDPD
                     KCWESDAGCPLLTSLAQELGLKADQFKADMKSCTSVVQNDMRALQGLGVGATPAFFIN
                     GRYISGAVPIDNFVTVIDEELKKANERIQQGTPQANYYQQWVMDKGLKTLEAPKQ"
     gene            complement(17066..>17933)
                     /locus_tag="VFV99_16525"
     CDS             complement(17066..>17933)
                     /locus_tag="VFV99_16525"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX5060975.1"
                     /translation="LVAQHRTFAAARRASRAPDDALALVRELGSRARLVVGDAAVGPH
                     VVGMLRPIVVVPPALLDDPALLRAALLHELAHVRRFDALGRYIQIIASSLMWWFPLMR
                     LVNRRLDLAREAACDAWALETGSVSRPAYARLLVRMAALRTAAAPTLAAHYALDARVA
                     AVLGPPARARIGVMHRLVLVAWFVVALGGARTASARSREQVCRYTPEMAAALYTAHPE
                     ADLDGDGLLSREEACDLQAQLQQSSSRLTPEREAALQTLLAEPLCCNCDLLDGYAAPE
                     PTTCLKAEGVDR"
BASE COUNT         3010 a         5968 c         6001 g         2954 t
ORIGIN      
        1 gcacgagatc acctcgccgc cgagcttcgc cgcgagctcc agcgagagcc gggtcttgcc
       61 ggcccccgtt ggtccgacga tgacgacgat gcgcggtggc acgcgcccat tgtagcgctg
      121 tgcgccaatc gcgacgaatc gcgttgaccg caggactggc acttgaccag agagaccacg
      181 cgcgcgcgga ccggcattca tccgcgttgt cgacgaaaac atcgctatca tgcgcgccat
      241 gcgtggactg gttctcgggc ttttcgtgat cgcggcatgc agcagtgaca gcacgccgcc
      301 cggcaagatc tatggcggcg atcgcccagc cgagctgaag acaccggcca cgctcaccga
      361 aggcaagcag tatccgttgc tggtcgtact gcatggcttc ggcgcgagtg gcttcgtgca
      421 gaccgcgttc ttcggcgttg gtggtctgcc cggcgaagac aaggcactgc tgatcgcacc
      481 cgacggtacc gagaactcga ctggccagca gttctggaat gcggatccgg cgtgctgcga
      541 tttcgagcat cacaatcccg acgacgtcgc gtacatcggc gggctcatcg atgacatcag
      601 tgcggactgg cccgtcgaca agaaacaggt gttcatcctc ggccactcga acggcggcta
      661 catggcgtac cgcatggcgt gcgagcgcgc cgatgtcatc gccgcgatcg cgtcactcgc
      721 cggcaacgcg tcgtcgacgt cggcgaattg caatccgtcc cagaaggtca acgtcgtgca
      781 cctgcacggc accgcagacg acaccgtgcc ctactccggc gcgacgccga gcgtcgatca
      841 gtgggccacc aagaacggct gcggcacgac acgcagcgag acgacgacgt acgacttcga
      901 cagcgccgtc gagggcgccg agacacacgt caatccgcaa gccgactgcc cggccaacgg
      961 cgacgtcgag ctgtggacga tggaaggctc gacgcacatc ccggtgatga acgcgacgtt
     1021 cgcgccgacg atcttcgact ggttcaccgc gcacaagcgc tgacgcttac agacgcctta
     1081 cgccggaacg cgcacgcgtc gtgcacgctc ttcgcatgac gcccatcgct cgtgtcgtga
     1141 gctcgccgct tgcccagctc gtatcgcttg tcgccgccgc cgcactgctc gtgatgatcg
     1201 tcgtcacgca gctgcgaccg caaccctcgg tcgtcgtcat gatgccgccg gccgcacccg
     1261 tcgtgggtca gccagcagcg gcgcctgtgc cgatgcccgt tgtgttcccg tactgctaca
     1321 gctgggcggg gagctggtac ggctactgcc gcgactagat ctcgatgccg atcagtgtat
     1381 cggcggcggg ttgcggattt tcgccgcctc gcccgggcac agcgtcgcgt actccgtcac
     1441 tagcttctcg agctcgggct cgggacgccg cccgaatgac tcgaacgcgg tcttcgcgaa
     1501 cagctgcatg cagttgatct cgcgcgtgtt gggatattgc gcgttgcgcg cgttcacgtc
     1561 ggcaatcgct ttgcgcaaga acaacttcgc accctcgcca tcgcacagcc gctcgatcct
     1621 cgcgacggtc gtcgcatcca gtcgcggcaa actcgcccgc agcgcagcgc actcgaccgt
     1681 gacgcccgac tccgaaccgt cggcgagcgc tttctcgagc gcggtgatct ctttgacgac
     1741 gaacttgcgc tccatctcgg cttcgtcaga cttcgtgcac gctgtgacaa cgagtgccgc
     1801 gcacaccaag ctcgcgatcc gcatacgcaa ggcgtagtct agctcagcga ccgaagcggc
     1861 gcgcgatgtc tgcgagcgac atccgcagca tcacgggccg accgtgcaca ccatgcgacg
     1921 agaagtcggc gccatccatc gagcgaaata gcgcttcggc ctcgctcggc gacaaccgat
     1981 cgcccgcgcg cacgaccgag tgacacgcga tttccgcgag cacgcgctcg agccgctccg
     2041 cttcactcgg cgcgccttca tccgcccaat cggtgagcag cttgcgcagc aggtgcgtcg
     2101 gatcgccgtg gcgaatgccg cccggcaccg ccttcaccgc gagcgtgcct ttgccgaagc
     2161 tctccgcttc gtagccgacc tgcgcgagca cgtcggtcat cttcgcggcg acatcgagca
     2221 gctcgggctt gagctcgatc gtcgtcggga acagcatgcg ctgcgtcggc acttcgcgtt
     2281 cgccctgccc tgccttgagt cgcgcgagct cgacgcgttc gtgtgccacg tgctgatcga
     2341 tcagcacgag ctcgccgtcg ccctcgcaga cgagatacgt cagatcgagc tggccgagat
     2401 acttcagcga cgcgaagaat cccggcgcgg tcgccggcgt gctgccctcc gcgatctgcg
     2461 cctcgtccca cggcgcagcg tcgcgctcct gctcgcgctc gacgcgattc agtgcgtacg
     2521 ccgcacgtgc tgcgagctcc gcttcggccg ctcgcgcact gcgcacgcga tccttgatct
     2581 gttgcgccca ctggcgcgga ccatcgtcga agccgagctg cgcctgcgca cgatcacgcg
     2641 tccactcgcg ctcgcgcgct tcccgcagct gcgccgcgta cgtttgcgcc atcgcagtcg
     2701 ccggcgcatc gaacggtagc ttcggcggcg cgacgctcgc gatcgccgtc atcatcaccg
     2761 gaccagcgcc accgatctcg tcacgccatg gcgctttctc gatgccagct tgcacgacgt
     2821 gacgaacggc cgcgcacacc gcgcccgcgt cggcgaaccg cacctcgtgc ttttgcggat
     2881 gcacgttgat gtcgaccgca cccgccggca cgtcgaccat cacgatcgcc gtcggataac
     2941 ggccgcgcgg caccagctcg ccgtagccca tcgcgagtgc gtggagcaag ccgcgatcgc
     3001 gcaccggccg cttgccgacg aacagctgca cgccgcgcgc ggtggtctgc gcgagctcgg
     3061 gcgcgccgag atacgcgatg acgcgggtgc cgccctcctg acccgagacc ggcaccatgc
     3121 gcgacgcgat ccgtgcaccg agcaatgact gcacgcgcgc aaagccatcg cgatcgggcg
     3181 gcgtgtcgag cgcgacccgg ccgttgtgct tgaggcgcag gtggagctcg tgatggacca
     3241 tcgcgacccg cgcgaccagc tcggtgatgt gcgacgcctc ggtggcctcg cccttcatga
     3301 acttcaggcg ggcgggcacg ttgtacagaa ggtcgactac ctcgacacac gtaccgacgg
     3361 gcgcgccgat ctcgctgacc tcgacgagct tgccgccctc gatgtgaacg cgagtagctg
     3421 cgagatctcc ggcctgccgt gtggtcagag tgagccgcga gacgctggca atcgcgggca
     3481 atgcctcgcc gcgaaagccg agcgttgcaa gtccccataa atcatcgagg ccgcgcagct
     3541 tggacgtggc gtggcgctcg aatgcgagca ccgcgtcctt ggggttcatg ccgcggccat
     3601 cgtcgacgac ccggaccaga ttgcgcccgc ccgccagggt ctcgaccgaa atggtgcgcg
     3661 cccctgcgtc gatcgcgtta tcgatcagct ctttgacgac cgaggacggc ctttcgatga
     3721 cctcaccggc cgcaatctga tcgatgacgg tgggcggaag aaccgcgatc actgtctcca
     3781 cccgccgtgc gtaacatggc cccctgatca gcccccagat cggacccgga agtgcgctat
     3841 aaggcccccc atgtcgagca caaaaccagg ccgcgtcgtt gcggtgaccg gggcgtgcac
     3901 ctacctcgga ctcgagctgc tgcggcggct cgaagaagac acccggtact cccgcgtgct
     3961 cgcgctcgac atccgcccgc ccgccgtgtc gggcaagaag atcgagtaca tcaagctcga
     4021 cctcacgcag ccgaccgtgg acagcgagct ggcgacgatc ctcgagcgcc atcgcgtcga
     4081 cacgttcgtg cacggcgcgt tcctgtcgca tccgacgcat gcggcggaat gggcacacga
     4141 gctcgaagat gtcggcacga tgcacgtgct caacgcgtgt gccggcgtcg agccgcgccg
     4201 cctggtgatg atctcgacca cgctggtcta cggcgcgcat cccaagaatc cgaacttcct
     4261 caccgaggaa gccgagctgc gcggccatcg cgattcgcgc ttcgtcaacg acaaggtccg
     4321 cgccgagcgc caggtgcagc ggttcgcatc cgagcacccg aacgtcgaag tctgcatctt
     4381 gcgattcgcg ccgatcatcg gcccgacggt gtcgaacatg tacacgcggt ttttccgccg
     4441 gccggtcgcg cccgtgatga tgggccacga tccgctgatg cagttcgtcc acgaacagga
     4501 cgccgcgttc gcgctcgtca aggctgtcga ctcgcacgcg accggtccgt tcaacatcgt
     4561 cggtaagggc gtgttgccgt atacgaccgt gctcgcgctg ctcggccgcg tgccggtgcc
     4621 gatgccgcag ctggtcgcgc gccaggtctc gaagatcttg tgggcgacgc agctcgtcgg
     4681 ctcgccgccg tcgttcctcg atttcttgct ctacctgtgc gtcgccgacg gcagcaaggc
     4741 gaagagcgcg ctcggcttct cgccgcgcct atcgatcaag cgcacgattc tcgatttcct
     4801 cggcgtcgcg ccggaggatg gagcgaccga cgtcgcgagg gcgcaggcct aagaccatga
     4861 ccgacgaacc cgacgacgaa agcaccgacg acgagtggta cggcgacgcc gatccgcgcg
     4921 tccccgatgc gacggcatgg ccggatcgcg atctcggttt cctcggcagc gactacggcg
     4981 gcaaggctgc ggtcgactcc gacatcaccg gccgcgcccg caccgccgac atcgatgaag
     5041 aggccgagct ccgcgagctc gagcgtcgcg tcgaacaaca gcgcacgcct gcgttcccga
     5101 tcgatctgcg cacacggctt ccgctcgaag gtctgtggcg gcgctggcgc gcgcttgcga
     5161 tgtggggccg cagcgacgtc gtcgatgatt tcggccgcga tccgcgtgcg accgcgcgct
     5221 gggaatggct gttcgagttc ctctactcga agtggttccg cgtgcaggcg atcggcgtcg
     5281 agaacattcc agcgcacggc cgcgcgctgc tcgtcgcgaa tcatgccggc acgctgccgt
     5341 acgacagcgc gatggtgatg cacgccgtgc gtcgcgatca tccgtcgcgc cgcgatgtcc
     5401 gcccgctcgt cgaggacacg gtgttccacc tgcccttcct cggcccgatc atgaaccgca
     5461 tcggcggcgt gcgcgccgat cccgcgaatg ccgagcgcct gctcgagaag gacgagctcg
     5521 tcgctgtctt ccccgagggc gaaaaaggca tgggcaagct gtggaaggat cgctatcgcc
     5581 tgcaacgatt cggccgcggc ggcttcgtca agctcgcgct gcgtacgcgc gcgccgatca
     5641 ttccggtcgc cgtcgtcggc gctgaagaag ccgcgccgat gctcggcaag gtgacgtggt
     5701 tcgcgaagaa catcggcatc ccgtggattc ccgtgacgcc gacgttcccg tggttcggtc
     5761 cggcgggcct cttgccgctt ccgagcaagt ggttcgtgca gttcggcgag ccgatcgatc
     5821 tcgccgcgca aaacggcccc gatgccgccg aggatcgcct gctcgtcaac aagctggctg
     5881 atcagatccg cacgcagatc caatcgatga tcgacgggct gctcggcaag cgccgctcgc
     5941 cgctgctcgc cgcgttcctc ggctgatgct gctcgatcga gatctcgagt tgctctacgc
     6001 gagggcactg atctcgatcg cgcgcgcgga aggcacgatc gatagcgatg aaggtgcgcg
     6061 ccttggcgag cgcatcgcgg agcgcagtgg gctcgagctc gcggacctct tgctcgagcg
     6121 ctcgctacag ccggaggagc tggcggatgc gctgatgcgg accggagccc catttcgcgg
     6181 cgcgtcggta cgtgcggatg aggtcggccg cgcgcttgtc gaggatgccc tgtatgtcgc
     6241 actcgcgaag ggccacgtga cccccgagga aggccatcgg atgtggcgct atgcgatcgc
     6301 cctggggctc tcggccgacg agtttcgggc cctgaccgca cactggctgc cgtaggggaa
     6361 ggatcctccc ggagggcacg tagccatccc gttcttgaac ggtgcgatcc gcgtgttatc
     6421 gtgaggccaa tgcctgcggc gtacgagtcc gagctagagc gtttgctcga agcggcgcgc
     6481 gagctgctcg gtgctggcga cgacgcactg ctcgtcgaag cgaacgcgac ggtgaaccag
     6541 gcgctgcagc tcacccccga ctgcgtggaa gcgtggctgc tcaagtgcca ggtcgcatcg
     6601 gcgaccggtg acgatctcgc tgcgctcgca gccgtcgaga tggcgctgca tcgcgcgccg
     6661 catcgcgccg aggcgttgta ctggcgcggc gccgtgctcg gcgatctcgg gcgtcatcgc
     6721 gaagcgctgc gcgcgatcga agccgcattg cgcgtgctcg gcgactccga tcactggctg
     6781 ctcgaagatc tgttctacga aaaggtcatg atcctcgatg cgctcggtct gcacgacgcg
     6841 gcggttgctg cgtgcgaagc gggcctcgat cgctgtcccg gctcgccgct cctgcgcgcg
     6901 gccctcgcgc ccgcagagcg cgcacgcgtg cgtagctcgt tgaaagtgct tcgcggcggc
     6961 gctggctagt ctcacgcaaa tttcgcgcag ctcggttgcg acgattccgt tgcagtccta
     7021 ggctcgcgcg cggaggagac tagagatgaa actcactacg tcgctcgtgt tggtgctcgg
     7081 cctggcggta ttcggctgca gcaagaagaa ggaagaggga accgcgcaga agtcggaacc
     7141 ggcgatgacg ccgaagacga cggagaagcc tgccgaaccg ccgaagccga cgccgctgac
     7201 cggcaccgcg ctggccgacc agtacaagaa gtgcgtcgac atggtcaacg ccggcaagtt
     7261 cgacgacttc aagaaggagt gcatcgacga cagcttcacg atgcacacgg gggccgggat
     7321 gcctgaaatg aagggcgcgg acgctctcgt cgcgcacttc aacgagatga agaccgcgtt
     7381 cccggacatg aagctgcaac cgcagctgat catggtcagc ggccgcaaca tcctcgccgt
     7441 caacctccat accggtaccc ataccggcac gatgaagacg ccgatgggcg acgttccggc
     7501 gacgaacaag aaggtcggca tgctgttctt ccatcgtctc gccatcaacg acgcgaacaa
     7561 ggcgacggaa gagtgggcca tgatggaccc gatgacgatg atgggtcagc tcggcctcaa
     7621 cccgaagacc gcgccgccgg tgcgtcccgc gatggacaag ggcatggagg gcgcgccgat
     7681 cgtcgtcgtc acggctgacg acgcgaagga gaaggcgaac atggacgcgg tcaagaaggc
     7741 caacgacgcg ttcattgcga acaagccggc cgatgcgatg gcggtgatga ccgacgacgc
     7801 agtcgagagc gatcaggcca ccgagaagga ccacaagggc aagaaggaca tcgaggccgg
     7861 cttcaagacg ttccgcaacg gctggagcga cgtgaagctg tcggatgtcc agatgtgggc
     7921 cgcgggcgac tacgtcgtgc agtcgttcaa gttcgaaggc aagaacgaca aagacatcgg
     7981 caagatgaag aagacgggca agaccgtcgc gctcgatgcc gtcgaagtca tgcacttcaa
     8041 ggacggcaag atcgatcagc tgtggcgctt catgaacggc atggacttcg cgcagcagat
     8101 gggcatgatg cccgcgcctg gcgcggctcc cgcaggtgcg aagccgggtg aaccgaaggc
     8161 tggtgaagca ccgaaggctg gcgaggcgcc gaaggccggc gagcccatga aaaaagagcc
     8221 cgagaagaag tagcgacagc tcacacctca gctctcggcg cgcccacgca ccgggcaacg
     8281 ggcacagcga tcgtcgcctt gctatcatcg cgaggtgatg tttcgccggg tcgccgggct
     8341 cgctgcgtgc ggcatggtcg ccgtagcggg cgtgtggtgg atcggcggtg cgatcgcggg
     8401 cgacgcgcca tcgatcggcc cgccgccggc cgatgcacac gtgctcacgg aagcagcgcg
     8461 tgcgggccgg cacgtgcgca tcgacggtcc gcgcggtgcg atccacgtgt ggattccgca
     8521 gagctatcgg cccgagaccg gcgcgaccat cgtctacgtg cacggctact acgacgacgc
     8581 ggacaccgcg tacgtcggcc atcggctgcc ggagcagttc gcgatgagcg cgctcaacgc
     8641 gatgttcatc gtccccgagg cgccgtacat gcagaaggtg ccggtgaact atccgaatct
     8701 cagcgagctg atccggctcg tcgaggacaa gaccggagtc tcgcgcggca tggcactgac
     8761 tgccgcggtt ggtcacagcg gcgcgtaccg cacgatcaat agctggctcg acgagccact
     8821 gctcgaccag atcgcgctcg tcgacgcgat gtacgcgaac gaggaagtga tcgaggcgtg
     8881 gctgcgggcg tcgccggtgc accgcatgat cacggtcggc gaggacacgc tgcagtggaa
     8941 cgagcagctg cttcgcgaca tgcccgacat gttcgtgatc gaccgcgtcc cgccgacgtt
     9001 cgacacctgg cccgccgagg cgaagaccgc gaaggccgtt tacgtgcgag cgcagtacta
     9061 tcacatgcct ctcgtcaccg acgggatcgt gctgcccggg gtgctgcgaa tgctgccggt
     9121 ggaattactc gcagacgagc catggcgaca gccactgggc gcgatgccgc cgctaccgga
     9181 tgctgcagtc gatgccgtgt ccggtgacgc tgtcagtgac tgacctataa tgcgcgtaga
     9241 tgcgtacgcg gatcgcagca ctcgtcgccg ccggtgcgct gctcgctttc gcgtacgtga
     9301 tgccgggcga gcgccggcgg ttcatgcaga aggtgatcac gtgggacgcc gagccgagcg
     9361 aggcgatcgc gctgcagccg ggcatgggcc tcggcatgac gccggcatcg cgcgtgcgcg
     9421 tgatcttgat cgacgggctc gcggagacga ccgccgagac gctgccgaac tggtcggccc
     9481 agtgtaagcg cggcatcgcg ctgacggtcg atgtcgggtt tccgaccgtg tcactgcccg
     9541 tggaggtcac gctgtggacc ggtctcatgc agcaacagac cggcatcgtg tttcgctcgg
     9601 accgtccgct cgtgccgccg ctgcagcaca gcatcgcggc gcaggtgccg gggagccgtg
     9661 cggtcgccga ggatcacggc tacatcgttc gctcgctcgg ttttgccgac acgaagccgg
     9721 tggcggtcga tccagcgcat ccggggaagg acgctgatcc cgaggcgtgg cgagcggcgt
     9781 ggctgccggc ggcacgcgac gcggtcacga gcgatgcgcg gctcgcgttc gtccacgtgc
     9841 tacgcgtcga tacctggggc cacaagaagg gccgcgactc cgacgaatac cgcaaggcag
     9901 cgagcgaagc ggacgcgatc gtcggtgagc tcgtcgcgct cgcgcccgat gcgcgctggt
     9961 tcttgctttc ggaccacggt caccttccga cgggtggcca cggcggcgaa gagcgcttcg
    10021 tgcggcaggt gcagcactgc atcatcggtc ccgagctcgc gcccgcgcac ggtggcccgg
    10081 tacacctcgt cgacatctcg cgtgcgatcg ccgactcggt cggcgtcacg ccggatcgcg
    10141 cgtcacaggg tcgcccgctg acggtcgcga tgcatgcacc gcttgaaaat gatgatgccg
    10201 tgccgccgct cgcgctgccg atcggcgcaa tcgcgatctt catcctcgcg cttggcctcg
    10261 ccggcagctc atttggcgtg cggcggtggt ggatggcgcc gtggtggttc gtgctcgcgg
    10321 gcgtcgcgct cgtgatctgg cgcggtcagc cgacgatgtc gacgccgatg gtctacgcgc
    10381 gagacagctt cttcgacatc ttcgatttcg atcgccgcct catgtttcgc agctggctcc
    10441 tcgtgctgcc gatcgccgcg atcacgacgt ggtacggctt ggctcgcaag acactgtggc
    10501 gcgtgctcgt cgcgcagctc gcgctaccat tcgcggctct cgcggcttgc atcacggcgt
    10561 gcgcgggctg gggagcactg ttcggcggcg aaatcgcgcc ggtcgtgccc cgctacaccg
    10621 cgtacacgtc agcgctgatg ctgatcactg cccacggggc ggccgtagtc gggctaaccg
    10681 ttctcgctcg gaccgcccat tcggcgtccg gtcaacgacg acctgcggaa acgtcgcgta
    10741 ctgccccttc aagcgagtga cgacgtcggg ctgcttggtg acgatgttgt cgagctgccg
    10801 cggatccttc tcgagatcgt agagctcctc gatgttatcg gacggccgca cgatcagctg
    10861 atacggccac tcgacaaccg accactgcag ctcttcgtgg atcgcgaccg agcgcggcgg
    10921 gtgacgtagc gccgcgggcg cgtcgagcag tgacggcacg agatcgatac catcgagcgg
    10981 catgtcgctc ggcgtcacgc ctgcgagcgc gaggagcgtc ggcgcgagat cgacgagcga
    11041 aatctgatcg gtgcgctgcc ccggcgtcac accgggaatc ttgaacgcga ccggaatgcg
    11101 cacgagcggc gcatacgtca ccttgccgtg cgtatcgagg agtcgcggat cctcgccgag
    11161 cgactcgccg tgatcgctcg caaacatgat gatcgtcttg tcggtcaggt tgcgcttcgc
    11221 gagctcgtcg acgagccggc cgacctcgtc gtcgatcgcg cgcagcagcg cacggtactt
    11281 cgtgaccttc ggcgtcgcgc ccgcgtgcac cgcctcgcgc aggcgcttcg gcacgtcgat
    11341 ctgatggtgc tcgtggacat cgaagtagtg cagccacaga aaccccgggc gatcgccgag
    11401 cgcgtcgagc gccttgatgc cctcgctcgt cgtcgccgcg gcactcacgt gatcgccgac
    11461 gtcggcggta tcccagtcgg tgtgcacggt agcgaccttg tcgatgccgc ggccgagcag
    11521 aacttcaccc gcgtagcgaa gcacctcggc gggaagcgcc gcgaacgtgt ggcgcccgga
    11581 tgcctgcatt gcctcgggca gcgtcatcgc caccggctgg aatggatcga agcggccggt
    11641 caggaaggtc gagagcgaaa cgtcggttcc cgatgcgggc gcgaacgcgt gcgtgaacca
    11701 cactgattcg gagagcagct tcgtgatcgc ggggaagtcg gcgcggtccg gcgcgtccgg
    11761 cgcgagtagg tcggcgcgca gcgcatcgac ggagatcacg atcacgtgca tgccgcgcgt
    11821 cttcgcgagc acgtcggcga tcgctggggt cgcacgccag ctcgcgaggt cctcgcgttt
    11881 cggtgcaggt ttcggcgcga cgctggtcgg ctgcggcgcg tccttgccgt cgcaatcttg
    11941 atcgatgcca tcaccgggct tgtcgacggc gcccgggtga cggctcgcat cggtatcgtc
    12001 gcaatcgcca ccgccgagca ctttcgagct gccgtcgcga tcgaagtcga ggacgtggcg
    12061 ccacaggttc acgatgtcgc ggctctgatc accgtacgtc gcgagcaact ggcgatccac
    12121 cggcgatttc agcccgccga ggcacgcgat gatcgcgccg ccgagcgcga gccccatgac
    12181 gacagcggcg atcaggtatg gcagcccgcc gcgtcgcgtg acacgcaccg cgacgccgat
    12241 gagcacgatg aggccgagcg tcgcgccgag gtgcgcggtc ggatacccgg tgccgagcac
    12301 gtgccgctcg acccagatca cggtgcccat catgccagcg agcgcgagca cgacgatgcc
    12361 gcgcgtcgtc aggtcggtcg cgcgcagaac gcggcggccc agtgcgacgg cacccgcaga
    12421 gagcagccag agcacgagcg gtaacaagaa tggcgccgcg cctgacagcg gcagtgtctg
    12481 tgcgtacgcg cctcgaaaca gcgagatcgc gacgacgacg aacacgacga gcgcaggcgc
    12541 tgtgagcacg agcgccgcga gccaccacgg gcgatcgacg acgaggcgct gcatcagcgc
    12601 gacgatcacg ccggtgatga gtcccatggc ggcgaacagc ggcacgacgg cgagcgcgat
    12661 gccgagcgat ccgaggcgtg cagaatcaac gctgcccagc gcggccgcgc cgagcgtgtg
    12721 cgcgacgatg atccaccacg tgcggagatc cggggcgggt gccggagctg cgtcgggcgc
    12781 gagcgctccc gacgacgaga tgcggtccga cgagatccgg cccgaggacc cgtgaaccgg
    12841 cgctgccagc ccccggtccg acggcgtcga gccgcgatcc ggcggtgacg cccggcctgg
    12901 tggcgacaac cgtcccggcg gcgataaccg ccccgacggc gtcgagccac gcgtcgctgg
    12961 ccatgacgaa ttcgagctgc gacgttgatc gggcaacagc ggcgatgcgc ggcgcagcgg
    13021 cggttgcgat ggcgtcgagc cgcgaccgtc gggcggctgc gatggcgtcg agccgcgacc
    13081 gtcgggcggc tgcgatggat acgacccgcg tgcatccggc ggtgcgctcg gcgtcgacga
    13141 ggtgcccggc gacggctgtg gttcggggcg cctcgggctc ctgccacggc gtgacgacga
    13201 atcgtcgctc acgtgacact cctggcgcga gaatgcaggt gtgccctcgc cgcgcgagct
    13261 tccccatggc tcgaacgcac agacgacggc atccgcccct gatcacagca tgtgcgccgg
    13321 gagcgcgcta cggcaaggac cgcctactta ttgggagggt atttttgcaa tttgcgttgc
    13381 aaactgcgtc gatggatacc gaggcgacgc gccgcctccg agatgttgcc gccggcgtcc
    13441 gagagcactc ggttgatatg ctcccactcc gcgcgcgcca gtgatggcgc cttgtagtcg
    13501 tgatccgctg gcggctcgag cggcggcgct tcgccacgcg cgaatgctgc gacgatgtcg
    13561 tcggcgtcgg ccggcttgga gaggtagtag gtcgcaccta aacgcaccgc atcgatcgcg
    13621 gtcgcgatgc tcccgtaacc cgtgagcacg acggtcttcg tcgcaggatc gatctcgcgc
    13681 agcgccttga ccagctcgag cccgctcttg cccggcatct tgagatcgac gactgcgaat
    13741 tcaggcgagt cggtggtcgc agcagcgacg gcactgtcgt agtcctgcgc agttcgggtg
    13801 tcgtagccgc gatcgacgaa cgcgcgcgcg aggcgggtgc gatacacctc gtcgtcgtcg
    13861 acgatcagga tcgatggttt gtgctcggcg ttgctcatcg cgaaactctg gggccgtcgg
    13921 cactcatgca ggcttggata ccactcggcc agttgtcgat tcggacgttc gcacgggttc
    13981 gtgggctgcg acagatgacc gcgcccctac gtcactaggt aggcgtacct ggacttcggt
    14041 acctccgcca ggggccgagt cgatctgcat ggttccaccg acgccctcga tgacggcgcg
    14101 cgccagaaat agcccgaggc ccattccgcg gccgggcgcc ttcgtcgtga agaacggctc
    14161 gccgatacgc tctttcacgt cgctcgggat gccttggccg cgatcgcgaa tgccgactgc
    14221 cagcgtgtcg tcgtcgaaat gcgcggtcac gacgacggcg gacgtcggcg gcgatgcatc
    14281 ctgggcgttg gtgacgagcg aacgcagcgc ctgactgacc gcgcgcggcg gcagacgcag
    14341 cgcgtggcga gcgacgtcat ccgtgatctc gttcagcacc ttcggcgcgt cgcggatgcc
    14401 gacgagggtc tctgcgatca gctcggcgac cgtgcacgac gtgacgctct cgcccacggt
    14461 gcctgcgccc tgcgccatct gctcgaggat cgagcggcag cgaccgactt gctcgcgaat
    14521 cagccgcgcg tccgaagcga gctcggcgtt cgtgctcgcg agtgcacgct cgagctcctt
    14581 cgcggcgagc gcaacggtgc cgagcggcgt cgatagctcg tgcgccgcac cggcggccat
    14641 cgtcgcgagc gatgcgagac gctcctgacg tgccgcgagg ccgcgcgctt cggtcagctc
    14701 gcgttcgcgc tcggcgacgg cggcggtgat gcgcaacagg aagtgaacga cgaacgcgga
    14761 tgcgacgccg agcgcgaccc acgcgccgta ggcgtgactg gactcgggaa tcctcagcgg
    14821 ctgatgcgcg acgagcagga tgccgaagcc gatgaacgag agcccgacga gcatccaggt
    14881 ccagcgcgcg cgcacgacga cggtcgcgag cgcgatctgc acgaggtaga ggaagctgaa
    14941 cggattgtgc ggaccaccag tgagatagag cagcgcggtg aggctcgcga tgtcgaacat
    15001 catcacgagc gcgagttgcc aatcgtgcac cggcttgggc tcgacgcgac cgcggcggcg
    15061 atcgccgaag aagtacatct cgatcgcgac gttcgagatg agaccgatgc cgatcacggc
    15121 gagcagcggc gcgatcggca gcgagccacc gaggagaaac tgtgccgcga tcacggtgac
    15181 cgcctggccg gcgacctccg cccatcgcag acgcgcgagc cactggatgt tgacccagtt
    15241 gcgctcggga atcgtcggcg gcggtgccac gggcgaaccc tagcaccgaa acgaaagaag
    15301 ccgggcgagc ccggcttcgc acggtccttg gtcaggactt actgcttcgg cgcctcgaga
    15361 gtcttgagac ccttgtcgac gacccactgc tggtagtact gcgcggccgg cgtaccctgc
    15421 tggatgcgct cgttcgcctt cttcagctcg tcgtcgatca ccgtggtgaa ctgatcgatc
    15481 ggcaccgcac ccgagatgaa gcgaccgttg atgaagaacg acggcgtcgc gccgacaccg
    15541 aatgcctgca ggtcgcgcat gtctttctgg accgccgcgg tgcagctctt catgtccgcc
    15601 ttgaactgat cgaccttgag gccaatctcc tgtgcccagc cggtcaagag cgggcagcca
    15661 gcgtcggctt cccagcactt gtcgggatcg aactggcggg ccttgaagcc cttctcccag
    15721 atcgcgttat ccatttcctt gtacttgccc tgacgagccg ccgcgcacgc cgcgagggca
    15781 ggccccatcg cggtgttcgg gtgcacgacc atgtgcttct ttacgacgcg aatatccttt
    15841 ccgtacttct gcgagagctg ctcgagggtc gcacggaccc tctcacagaa cggtcaagca
    15901 tagtcgcttg cttctactat cgttaccttc gcgtcggccg ggccctcgaa cgagtcgccg
    15961 tcgatcggca cagcataggt cttcgcgcga tccggctcgg gacgcgacgg gcgctgcgca
    16021 ccgggctgag caccacgacc gccgccctga gcgagcagcg cgtctagctt cttgtcgagc
    16081 ttgtcgatct tttcgttgag cttcgtcgtg tcggcctgac acgccatcaa cagagcgcca
    16141 cacaagagaa ctagggcttt catcgctcga ctccttgagt ttctaggtga gtagacctct
    16201 gggcggtccg aattgcaact cggcgacacg gtaaggaatg ttgcgaatta ctgcttcggc
    16261 gcttcgaggg tcttgagccc cttgtccatc acccactgct ggtagtaatt cgcctgcggc
    16321 gtcccctgct ggatgcgctc gttcgctttc ttcagctcct cgtcgatcac ggtgacgaag
    16381 ttgtcgatcg gcaccgcgcc cgagatgtag cggccgttga tgaagaacgc aggcgtcgca
    16441 ccgacgccga gaccctgcaa cgcgcgcatg tcgttctgga cgactgacgt gcagctcttc
    16501 atgtccgcct tgaactgatc ggccttgagg ccgagctcct gcgccaagct cgtcaggagt
    16561 gggcaaccgg cgtcgctctc ccagcactta tcggggtcga actggcgagc cttgaagctc
    16621 ttctcccaga tcaggttgtc gtactgcggt gccttgccct gcttcgccgc cgcgcagaat
    16681 gcgagcgcgc cgctcattgc ggtgttcggg tggacgacga gctgcttgta gacgacgcgg
    16741 atgtcgttgc cgtacttctt gtggaggtca tccatcgtcg agcgtacgcg ttcgcagaac
    16801 gggcacgcgt agtcgtacgc cttgacgacc gtgatcttcg cgttcgccgg gccgtcgaac
    16861 ggatcgttgt cgatcggcac tgcataggtc ttcgcgggat caggtccggg acgcggcgca
    16921 cgcggctgct gagcagcacc gggacgaccg ccctgcgcga gcagcgcgtc gagcttcttg
    16981 tcgatcttgt cgaccttttc gttgagcttc gtcgtgtcgg cctgacaggc catgagagca
    17041 gcaccaaaca acagaacaag ggccttcatc gatcgactcc ttcagctttc aaacaagtcg
    17101 tgggttcggg ggcggcgtag ccgtccaaca agtcgcagtt gcaacacaac ggctccgcga
    17161 gcagggtttg gagcgcggct tcgcgctcgg gcgttagccg agacgagctc tgctgcagct
    17221 gcgcctgcag atcgcaggct tcttcgcgag agagcaggcc gtctccgtcg aggtccgctt
    17281 cgggatgcgc ggtgtagagc gcagcggcca tctctggggt gtagcggcag acctgttcgc
    17341 ggctgcgtgc actcgcggtg cgcgcgccac caagagcgac gacgaaccac gcgaccagca
    17401 cgagacgatg catgacgccg atgcgtgcgc gtgccggcgg gccgagcacg gcagcgaccc
    17461 gtgcatcgag cgcgtagtgc gcggcgagtg tcggcgcggc agcagtgcgc agcgcggcca
    17521 tgcgaacgag caagcgcgcg tacgcgggac gcgacaccga gccggtctcg agtgcccacg
    17581 catcgcacgc agcttcgcgc gcgaggtcga gccggcggtt gacgagccgc atcagcggga
    17641 accaccacat cagcgagctc gcgatgatct ggatgtagcg gccgagtgca tcgaaccgac
    17701 gcacgtgcgc gagctcgtgg agcaaggccg cgcgcaacag cgcgggatcg tcgagcagtg
    17761 ccggcggcac gacgacgatc ggccggagca tgccgacgac gtgcgggccc accgcagcgt
    17821 caccgacgac gaggcgtgca cgcgagccga gctcgcgaac gagcgcgagc gcgtcgtccg
    17881 gtgcgcggga ggcacggcga gcggcggcaa acgttcggtg ctgtgcgacg agc
//
LOCUS       DASUFX010000413         9973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1734965, whole genome shotgun sequence.
ACCESSION   DASUFX010000413 DASUFX010000000
VERSION     DASUFX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734965"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFV99_16530"
     CDS             <1..1398
                     /locus_tag="VFV99_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5060976.1"
                     /translation="VADRRATAADIVTSVQRDIESGALPAGSRLPPVRALEKQLGLSK
                     NTAQAAYDELVARGLVEAREREGVFVLAASRFASPGTMIAAPPAAFVAPPLSRPDVAP
                     RGMTALSTVFIDPELLPTERIAECARSVLRERMPSHYDAQGYRPLRESIAKRLQVRGL
                     DVEADEIVITTGSQQSLDIVARSLAVKRIAVESPLYAYAKLLFETLGHELVGLHLDPF
                     GGIDLAAWERALATKPSLAYLIPSFHNPTGYSYTSAELRGVLELCVKHGVAILEDDWG
                     SDMLSDGEYRPMLRMLGGKDVLYVNSFTKKLLPSLRVGFVAASPALVPTLVAMKRLST
                     LGNAWLTEAIVAEFLDRGYYDTHLQALQRALDARYAACLAALDELMPDGVRWTHPGGG
                     PTLWLDIPRAIDLPALEAHLARRGIHISNSSAAFVGPAHLHGFRIAYAYLPEEEMRRA
                     LTIVADALRELGRAR"
     gene            complement(1406..2188)
                     /locus_tag="VFV99_16535"
     CDS             complement(1406..2188)
                     /locus_tag="VFV99_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060977.1"
                     /translation="MKRAQKICYVVASVFATYALAGSAAAQDSQPPQQQPQQQTPSRQ
                     KAPQPSDQQGMDKEKQDKQAAPGEVMAARMSLTATVDKIDMKKRQLTLKDDSGNQFKV
                     DVPPDVQDFKNIKKGDKISIDYYSSVALKLQKPGAAGKPGMTETEMAAKEPAKLPGGI
                     VARKITATAEVMKVDTSANTVTIKGPGGELDTIKVDDPDVQADLGKLKKGDKIKASYT
                     EAVAISITPAQKMGQGPQVAPEKTPDQAPEQKEPKTDTNKGY"
     gene            2282..3511
                     /locus_tag="VFV99_16540"
     CDS             2282..3511
                     /locus_tag="VFV99_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060978.1"
                     /translation="MHYRVLAVLALVACSSAPAATTDFWGPTIEPPRGLGKLAPGMSV
                     ADAIKLVPALHEPKARGVRDELILDSGVGDVKLAVRVEAGTVSSILAIVQNPSVRDLL
                     TRAWGQPEITRDSLGQPEVTWASEATGWKVKLDCMERNCLVEYVPYHVLTAEFFGPHV
                     VPPGDLGKLRIGMPLAEARTLAPGPVSARSGIATKYDGVREFVAIDDKQGTVRSIYLN
                     LPEDAVELITAAWGDGAAAAMPVGKAVEVWHDPETGWRATLRPALGRSRDLVFDIYMP
                     AFVLFGDQPDTLEGLREPVLGRSVDDVKKAYKDVITAQGRDLVVSLPPTEWDRFATRI
                     LLDTSGGRVRSLSFAIPFKPHPQARDQLLDLFKGKWGLPEEVVEDSKPTLVFRKDDPR
                     VEIREDNEHGAWLVEIR"
     gene            complement(3512..4117)
                     /locus_tag="VFV99_16545"
     CDS             complement(3512..4117)
                     /locus_tag="VFV99_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060979.1"
                     /translation="MTYRSDHEAALARVDALEHELAKLRKPAEKPRPRPKSRFAWFAA
                     SIAAAAGVFVGLTPAMCGGTDETQPAELDRLDRTRLVKCVEAVELTPLLTAAETDPHR
                     ETPKPITAIVPTAAPCREDLHTIVESSPLSPNERVALSTWVSYEDELAGAIARIETYY
                     ASDPYKLDNYTSARQLWVEYDRAHDQRDVAMLTWRRDFAKL"
     gene            4320..5108
                     /locus_tag="VFV99_16550"
     CDS             4320..5108
                     /locus_tag="VFV99_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5060980.1"
                     /translation="MLDTNRYRAAAARDPFRRPLVVVPSRTAIAINAVVAVGVIALGL
                     AQRLTPDVFGFFAIGLGIGVATCIPIGIANVIVIDAAYRHGARRALGASLGGALADGL
                     YASLGIFGIGPLIGRYPTVPYVLHAISGCVLLVYGVVLLRARPALAGPVESRSSGNDS
                     HLLGGFLVGLGATLLNPSAIVTWVVIVGGHATGITRVEASAWVVGIVVGTLAWFLVVM
                     GLALRGRRVLREKAVWMTRIVAVVVIGSGLLSFARVVDFVFGAR"
     gene            5190..5975
                     /locus_tag="VFV99_16555"
     CDS             5190..5975
                     /locus_tag="VFV99_16555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5060981.1"
                     /translation="MIDALRHRLQLLLYAWRTVGPGTMLRNTWRWALDGEARNSNESE
                     LDARFGIDTTAELTPNEAAIPVARRAVATMYLPSLGDDLDAMLAALAWPDSLARDATF
                     VDIGSGKGRVVLLAAMRRFREVIGVELSPVLHRIAGRNLDIVQGSGALVSPARLTLGD
                     ATELDVPHGPLIAYLYHPFRDPIAAIVVDRIVASLAASPRPAAILYGHPTLQQPIDPA
                     VFARGGVFDARAHGERRTRRYRIGWSVWTNDAWLAANRCLVGA"
     gene            5939..6568
                     /locus_tag="VFV99_16560"
     CDS             5939..6568
                     /locus_tag="VFV99_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5060982.1"
                     /translation="MAGSEPLLGGRVTMLAYYLLGVVLGAGSSVVPGPCGLAVIDAAT
                     RLGSRRAIATAIGSGLGDLTYAALGVFGIGQVLARDRELVAIMLAASGVVLIGYGLAC
                     LRGRPVASEAEARSLGGIVVGFATLVCNPGALLMWSSIGIQIATATRAEHVCAVFGIG
                     SGSAAWFVAMAVLSARCNRVLGERMHRVVQVIGCLIVAYGAVSLARAVL"
     gene            complement(6610..7230)
                     /locus_tag="VFV99_16565"
     CDS             complement(6610..7230)
                     /locus_tag="VFV99_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5060983.1"
                     /translation="MTPSHLQQVTTGGSFASRLRNAHNFVDVATIGCAAAQELGLHCV
                     ISLHGTNGQPSITVDNIADAPDNMFGFPTPILALDEHLGVVRWSAAAPLTPELRRELE
                     TMTMHISVRLAQLGYFAREDHSQRLTDRQNEAARLAARGYTNGEIAEELALSANTVKK
                     HLKEVFERLGVSSRTELAVRYARLAADDGVPDGVSYRAGCTLTKHR"
     gene            7372..8559
                     /locus_tag="VFV99_16570"
     CDS             7372..8559
                     /locus_tag="VFV99_16570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEX5060984.1"
                     /translation="MTTESKEPKRVSDLAYAATVAKSVVANVSNILKTTTVKRTLRTA
                     YNVARTTSWKKHVEYPVMVAIETTNKCNATCWFCPITEGSKRPPSDPAQLIQLGKKSE
                     PAPEPQADPDAASFNLMKRELGYMSDELFEKIIDDCKRFPLRRLEPYLHGEPFMDRKI
                     CERIEYINKHLPQTEVHIFTNGSLLTKKTVDKLKELNIASMVISLNTAVGDKYNAIMG
                     LDWKKTLANLERVAAENRTDKKVADRFVMRMTAPKETTDAEIAAFRKLAKQMGARAVI
                     VSLHNYKGDITSERPVPQFPCYFVNDLDILYDGRTALCCMDHDGDYSWGDVSKHSVLE
                     VYNSKEAKHYRTMMREDRRNELTPCNECNMFWWSLERTGVRRLQYSGRIAQYLIKHRP
                     LRF"
     gene            complement(8570..>9973)
                     /locus_tag="VFV99_16575"
     CDS             complement(8570..>9973)
                     /locus_tag="VFV99_16575"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5060985.1"
                     /translation="GTPHATVEDAQRFRAAYFTPDNATLVIAGHFDIALANKWIDFLF
                     ADWHGHAQPRHLTPPTPHPASIAQIENLTQLLVLAAVPVTTGTRAQRLVAAAMLAEIT
                     GDVRHQLGATYDFDAGLDENRLGARWIIGGWVEPGRANDVVALLRDRIEQLRKDPDAA
                     ARAFVLARARVVAHLASVTGSASLIAARAETDVAYDRAPMSDLATARAVQALTIDQMA
                     PLLAELDLSRAAVLMRGADEPIRQAFAVLGREPTRVHMEQVQVAEDASEDPPLSTTQT
                     PHFQRITFNDIDYAITDQGAAAKSPLEVGLAVTLAIGTVPDLFPGMQTLPVEGSPSGV
                     MVSGEVGLRFRARFSTGLQLGIGRFGGSYGLTSGGVAYDYLPYSIVPIDVAAYLHMTP
                     LANVWAGFLVGVHHDGIAFDADRTWTRSVGIGIEAGYDVYSLGPNSVAIVARATGAYG
                     GADPNLGSIGIGVAYHH"
BASE COUNT         1559 a         3203 c         3375 g         1836 t
ORIGIN      
        1 gtcgccgatc gtcgcgccac cgcagcggac atcgtcacga gcgtgcagcg tgacatcgaa
       61 agcggggcat tgccggccgg ctcgcgactc cctccggtgc gcgcgctcga gaaacaactc
      121 ggcctctcga agaacaccgc gcaagccgcg tacgacgagc tcgtcgcgcg tggcctcgtc
      181 gaagcgcgcg agcgtgaggg cgtgttcgtg ctcgctgcgt cgcggttcgc gtcgcccggg
      241 acgatgatcg cggcaccgcc ggccgcgttt gtcgcgccgc cgctatcgcg tccagacgtc
      301 gcgccacgcg ggatgaccgc gctgtcgacc gtgttcatcg atccggagct attgccgacc
      361 gagcgcatcg cggagtgcgc acgttcggtg ctgcgcgagc gcatgccaag ccactacgac
      421 gcccagggct atcgtccgct acgcgaatcg atcgcgaagc ggctgcaggt gcgcggactc
      481 gacgtcgaag ccgacgaaat cgtcatcacg accggctcgc agcagtcgct cgatatcgtc
      541 gcgcgctcgc tcgcggtcaa gcgcatcgcg gtcgagtcgc cactgtacgc gtacgcaaag
      601 ctgctattcg agacgctcgg ccacgagctc gtgggcctac atctcgatcc gttcggcggc
      661 atcgatctcg ctgcgtggga gcgcgcgctc gcgacgaagc cgtcgctcgc atatctcatt
      721 ccgagctttc acaacccgac cggctactcg tacacgagtg cggagctgcg cggtgtgctc
      781 gagctgtgcg tcaagcacgg cgtcgcgatt ctcgaggacg attggggcag cgacatgctc
      841 tccgacggcg agtaccggcc gatgctgcgc atgctcggcg gcaaggacgt cctctacgtc
      901 aactcgttca cgaagaagct gttgccctcg ctgcgtgtcg gcttcgtcgc ggcttcgccg
      961 gctcttgtac cgacgctcgt cgcgatgaag cggctatcga cgctcggtaa cgcgtggctg
     1021 accgaagcga tcgtcgccga gttcctcgat cgcggatact acgacacgca cctgcaggct
     1081 ctgcagcgtg cgctcgatgc ccgttacgcc gcgtgtcttg cagcactcga cgagctgatg
     1141 cccgacggtg tgcggtggac tcatccgggc ggcgggccga cgctctggct cgatattccg
     1201 cgtgcgatcg acttgccagc actcgaagcg catctcgcgc ggcgcgggat tcacatctcg
     1261 aactcgtcgg cggcgtttgt cggacccgca catctgcacg ggttccggat cgcgtatgcc
     1321 tacttgcccg aggaggagat gcgccgcgcg ctcacgatcg tggccgacgc gcttcgagag
     1381 ctgggccgcg cgcggtgagc gtcgcctagt accccttgtt cgtatccgtc ttcggctcct
     1441 tctgttccgg cgcctggtcc ggcgtctttt ccggcgcgac ctgaggtcct tggcccatct
     1501 tttgggcagg cgtgatcgag atcgcgaccg cctcggtgta gctcgccttg atcttgtcgc
     1561 ccttcttgag cttgccgaga tccgcctgga cgtcggggtc gtcgaccttg atcgtgtcga
     1621 gctcgccgcc cgggcctttg atcgtgaccg tgttcgccga cgtgtcgacc ttcatcacct
     1681 cggccgtcgc cgtgatcttc cgcgcgacga tgccgccggg gagcttcgcc ggttccttgg
     1741 ctgccatctc ggtctctgtc atgcctggct taccggcggc gcccggcttc tgcagcttca
     1801 gcgcgaccga cgagtagtag tcgatcgaga tcttgtcgcc cttcttgatg ttcttgaagt
     1861 cctgcacgtc aggcggcaca tcgaccttga actggttgcc actatcgtcc ttgagggtca
     1921 gctggcgctt cttcatgtcg atcttgtcga ccgtcgccgt cagcgacatg cgtgcggcca
     1981 tcacctcgcc gggcgccgcc tgcttgtcct gtttctcctt atccattccc tgctgatcgg
     2041 acggctgcgg ggctttttgc ctcgacggcg tctgctgctg cggctgctgt tgcggtggct
     2101 gcgagtcttg cgcggcagca ctacctgcca gcgcgtaggt cgcaaaaaca ctcgcgacca
     2161 cgtaacaaat cttttgggct cgtttcatgg cgagtcctcc tttcgaggac ccaggtggtg
     2221 caaggtcatg gccacctgcg agacgcttgt gcttcgagca atagcggggc agaaatgtag
     2281 agtgcactac cgggtcctcg ccgtgctcgc gctggttgcg tgtagttctg cgccagcggc
     2341 gaccaccgac ttctggggcc cgacgatcga gccgccgcgc ggtctcggca aacttgcgcc
     2401 cggcatgagc gtcgcggacg caatcaagct cgttcccgca ctgcacgagc cgaaggcgcg
     2461 cggtgtgcgt gacgagttga tcctcgattc gggcgtcggt gacgtgaagc tcgccgtgcg
     2521 cgtcgaagcg ggcaccgtgt cgagcattct cgcgatcgtg cagaacccga gtgtgcgcga
     2581 cctcctcacg cgcgcatggg gacaaccaga gatcacgcgc gactcgctcg gtcagcccga
     2641 agtgacatgg gcgagcgaag cgactggctg gaaggtcaag ctcgactgca tggagcgcaa
     2701 ctgcctcgtc gagtacgtgc cgtatcacgt gctcaccgcc gagttcttcg gtccgcacgt
     2761 cgtaccaccg ggcgatcttg gcaagctgcg catcggcatg ccgctcgccg aagcgcgcac
     2821 gctcgcgcca ggtccggtct cggcgcgcag tggtatcgcg acgaagtacg acggcgtgcg
     2881 cgagttcgtc gcgatcgacg acaagcaagg cacggtgcgt tcgatctatc tcaaccttcc
     2941 cgaggatgcc gtcgagctga tcaccgctgc atggggcgat ggcgccgccg ctgcgatgcc
     3001 cgtcggcaag gccgtcgagg tctggcacga cccggagacc ggctggcgcg cgacgctgcg
     3061 gcctgcgctc ggacgcagcc gcgatctcgt gttcgacatc tacatgccgg ctttcgtgct
     3121 gttcggcgat cagcccgaca cgctcgaagg tctgcgcgag ccggtgctcg gtcgcagcgt
     3181 cgatgacgtc aagaaggcct acaaggacgt gatcacggcg cagggccgcg acctcgtcgt
     3241 gtcgctgccg ccgaccgagt gggaccgctt cgcgacgcgc atccttctcg atacctccgg
     3301 tggccgcgtg cgctcgctgt cgttcgcgat tccgttcaag ccgcacccac aggcgcgcga
     3361 tcaactgctc gatctcttca agggcaagtg gggcttgccc gaagaggtcg tcgaggattc
     3421 gaagccgacg ctcgtgttcc gcaaagacga tccgcgcgtc gagatccgcg aagacaacga
     3481 gcacggcgcg tggctcgtcg agatccgctg atcagagctt cgcgaagtcc ctgcgccacg
     3541 tcagcatcgc gacgtcgcgc tggtcgtgcg cgcggtcgta ctcgacccag agctgacgag
     3601 cgctggtgta gttgtcgagc ttgtacggat cgctcgcgta gtacgtctcg attcgcgcga
     3661 tcgcaccggc gagctcgtcc tcgtagctga cccacgtcga cagcgcgacg cgctcgttcg
     3721 gcgacagagg cgacgactcg acgatcgtat gtagatcctc tcgacacggc gccgctgtcg
     3781 gcacgatcgc cgtgatcggc ttcggcgttt cgcgatgggg atcagtctcc gccgccgtga
     3841 gtagcggcgt cagctcgacg gcctccacgc acttgacgag gcgcgtacga tcgagccggt
     3901 cgagctcggc cggctgcgtc tcatcagttc ctccgcacat cgcgggcgtc agacccacga
     3961 acacaccggc ggcggcggca atggacgccg cgaaccacgc gaatcgagac ttcggacgcg
     4021 gccgtggttt ctccgcgggc ttgcgtagct tcgcgagctc gtgttcgagc gcatcgacgc
     4081 gagccagcgc ggcctcgtga tcgctgcgat atgtcattga cccctcctcc ttgctacttc
     4141 ggatgcaagc tgaggacgcg cagtcgcctc gtcgagagta aaagcgacac gtgtccaatt
     4201 tcggttgcgc gtcggcgatg ccgtgcttag cgcgcgtacc cgatcgggtc ctaccgatca
     4261 cgcgcggctc acgttacttg acctgttggg ctcacgtagg gagctgcgag tcagggggaa
     4321 tgctcgatac gaaccgctat cgtgcggcgg ccgcacgcga tccgtttcgc cggccgctcg
     4381 tagtcgtgcc gagtcgcacc gcgatcgcga tcaacgcggt cgtggcggtt ggcgtgatcg
     4441 cgctcgggtt ggcgcagcgg ctcacgcccg acgtgttcgg gttcttcgcg atcgggctcg
     4501 ggattggcgt cgcgacgtgc attccgatcg gcatcgcgaa cgtgatcgtg atcgatgcgg
     4561 cgtaccggca cggcgcccgc cgcgcgcttg gcgcgagctt gggcggcgca ctggccgacg
     4621 ggctctacgc gagcctcggc atctttggca tcggcccgtt gatcggacgg tacccgaccg
     4681 tgccgtacgt gctgcacgcg atcagcgggt gcgtgctgct cgtctatggc gtggtgctct
     4741 tgcgtgcgcg accggcgctc gccggcccgg tcgagtcgcg gtcctcgggc aacgacagcc
     4801 atctcctggg cggcttcctc gtcggactcg gtgcgacgtt gctcaatccg tcggcgatcg
     4861 tgacctgggt cgtgatcgtc ggcggtcacg ccaccggcat cacgcgcgtc gaggcgagcg
     4921 cgtgggtcgt cggtatcgtc gtcggcacac tcgcgtggtt tctcgtcgtc atgggcctcg
     4981 cactgcgcgg gcgtcgcgtg cttcgcgaga aagccgtgtg gatgacacgc atcgtggccg
     5041 ttgtcgtgat cggctcgggt ttgctgtcct ttgccagggt cgtcgacttc gtgttcggcg
     5101 cgcgctgacc cggccgggtg ccgcgcgtca cgcgttcttc acgacgacga ccctgatgag
     5161 ctcacgcacg actgcgtcag cgtgaggtca tgatcgatgc actcagacac cggttgcagc
     5221 ttcttctcta tgcctggcgc acggtcggcc ccggcacgat gcttcgcaat acgtggcggt
     5281 gggcgctcga cggcgaggcg cgcaacagca acgagagcga gctcgatgcg cggttcggta
     5341 tcgacaccac ggcggagctc acgccgaacg aagcggcgat tccagtggca cggcgtgcgg
     5401 tcgcgacgat gtatctgccg tcgcttggtg atgacctcga tgcgatgctc gcggctcttg
     5461 cgtggccgga ttcgctcgcg cgcgacgcga cgtttgtcga catcggaagc ggcaagggca
     5521 gggtcgtgct gctggccgcg atgcggcggt ttcgcgaagt catcggcgtc gagctgtcgc
     5581 cggtactgca tcggatcgca gggcgcaatc tcgacatcgt gcaaggcagc ggcgcgctgg
     5641 tgtcgccggc gcgcctgacg ctcggcgatg cgaccgagct cgatgtgcca cacgggccgt
     5701 tgatcgcgta cctctatcac ccgttccgcg atccgatcgc tgcgatcgtt gtcgatcgga
     5761 tcgtcgcgtc gctcgctgcc tccccgcggc ccgcggcaat cctgtacggc catccgacgc
     5821 ttcagcagcc gatcgatccg gcggtgttcg cgcgaggcgg agtgttcgac gcgcgggcgc
     5881 acggagaacg gcgaacgcgg cgctatcgga tcggctggtc cgtatggacg aatgacgcgt
     5941 ggctggcagc gaaccgctgc ttggtgggcg cgtgacgatg ctggcgtact acctgctcgg
     6001 cgtcgttctc ggcgcgggct cgagtgtcgt gccaggcccg tgcgggcttg cggtgatcga
     6061 tgcggcaacg cggctcggtt cgcggcgggc aatcgctacc gccatcgggt cgggcctcgg
     6121 cgatctgacg tacgccgcgc tcggcgtgtt tggcattggc caggtcctcg ctcgcgatcg
     6181 cgagctggtc gcgatcatgc tggcagcaag cggcgtggtg ctgatcggct atgggctcgc
     6241 gtgtctgcgc ggccggccgg tcgcaagcga agcggaagcg cgctcgctcg ggggcatcgt
     6301 cgtcggcttt gcgacgctcg tctgcaatcc cggtgcactg ctcatgtggt catcgattgg
     6361 aatccagatc gctacggcga cccgcgcaga gcacgtgtgc gcggtgttcg gcatcggcag
     6421 cggctcggcg gcgtggttcg tcgcgatggc ggttctctcg gcgcgctgca atcgtgtgct
     6481 cggcgaacgc atgcaccgcg tcgtccaagt gatcggctgc ttgatcgtcg cgtatggcgc
     6541 cgtgtcgctc gcgcgtgcgg tgctgtaatc gagcgcgatg ctcacgccgc gccgggcatg
     6601 gctccgcgtt cagcgatgct tcgtcagcgt gcagcccgcg cggtagctca ccccatcggg
     6661 taccccatcg tccgcggcca gccgcgcata gcggacggca agctcggtgc ggctcgacac
     6721 gccgaggcgt tcgaacacct ctttgaggtg ctttttgacc gtgttcgccg atagcgcgag
     6781 ctcctcggca atctcaccgt tcgtataacc gcgcgcagcc agccgcgctg cctcgttctg
     6841 gcggtcggtg aggcgttggc tgtggtcttc gcgggcgaag tagccgagct gcgcgagccg
     6901 caccgagatg tgcatggtca tggtctcgag ctcgcggcgc aattctggcg tcaacggcgc
     6961 ggcggcgctc cagcggacga ccccgaggtg ctcgtcgagc gcgaggatcg gggtcggaaa
     7021 cccgaacatg ttgtcgggcg cgtcggcgat gttgtcgacc gtgatcgagg gttgtccgtt
     7081 cgtgccatgc agcgagatga cgcagtgcag cccgagctcc tgcgcggccg cgcaaccgat
     7141 cgttgcgacg tcgacgaaat tgtgtgcgtt gcggagtcgg cttgcgaacg acccgccggt
     7201 cgttacctgc tgcaggtggg acggcgtcat gtcctcaagg tcgccggtgc gcgcgtgaaa
     7261 ttgcggtgaa gcgttcgcgc gaatcgggtg aggaaaggga cccggacggg taatagccag
     7321 cgtcgctggc tttgcggatt ctgcggcgat gttgggaaag ggtggaaccg aatgacaacc
     7381 gaatcgaagg agccgaaacg cgtctccgac ctcgcgtacg ccgcgaccgt ggcgaagagc
     7441 gtcgtcgcga acgtgtcgaa catcctcaag acgacgacgg tgaagcgcac gctgcgcacc
     7501 gcttacaacg tcgcgcgcac gacgtcgtgg aagaagcacg tcgagtaccc ggtgatggtc
     7561 gcgatcgaga cgacgaacaa gtgcaacgcg acgtgttggt tctgtccgat cacggagggc
     7621 tcgaagcgtc cgccgtcgga tcccgcgcag ctgatccagc tcggcaagaa atcggagccg
     7681 gcgccggagc cgcaggccga tccggatgcc gccagcttca atttgatgaa gcgcgagctc
     7741 ggttacatga gcgacgagct gttcgaaaag atcatcgacg actgcaagcg gttccccttg
     7801 cgtcgcctcg agccgtacct gcacggcgag ccgttcatgg atcgcaagat ctgcgagcgc
     7861 atcgagtaca tcaacaagca tctgccgcag accgaagtcc acatcttcac gaacggcagc
     7921 ctgctgacga agaagactgt cgacaagctc aaggagctga acatcgcgag catggtgatc
     7981 tcgctgaaca ccgcggtcgg cgacaagtac aacgcgatca tggggctcga ctggaagaag
     8041 acgctcgcga acctcgagcg cgtcgcggcg gagaaccgca ccgacaagaa ggtcgccgat
     8101 cggttcgtca tgcgaatgac cgcgccgaag gagaccaccg acgcggagat cgcggcgttc
     8161 cgcaagctcg cgaagcagat gggagcgcgc gcggtcatcg tgagccttca caactacaag
     8221 ggcgacatca cgtcagagcg accggtgccg cagttcccct gctacttcgt caacgacctc
     8281 gacatcctct acgacgggcg cacagcgctg tgctgcatgg accacgacgg cgactactcg
     8341 tggggcgatg tctcgaagca ttcggtgctc gaggtctaca actcgaagga ggcgaagcac
     8401 taccgcacga tgatgcgtga agaccgtcgc aacgagctca cgccgtgcaa cgagtgcaac
     8461 atgttctggt ggagcctcga gcgcaccggc gtgcgccggc tgcagtacag cggccgcatc
     8521 gcgcagtact tgatcaagca ccgcccgttg cggttctgag ccgcggcact cagtggtggt
     8581 acgcgacgcc gatgccgatc gagccgagat tggggtcggc accaccatac gcgccggttg
     8641 cgcgcgcgac gatcgcgacc gagttgggac cgaggctgta gacgtcgtag ccagcttcga
     8701 tcccgatgcc gacgctcctc gtccaggtgc ggtcggcgtc gaacgcgatg ccatcgtggt
     8761 ggacgccgac cagaaagccg gcccacacgt tcgcgagtgg tgtcatgtgc agataggcgg
     8821 cgacgtcgat cggcacaatg ctgtagggca ggtagtcgta ggcgacaccg ccggacgtga
     8881 gcccgtacga gccgccgaac cgtccgatgc cgagctgcag accggtgctg aagcgtgcgc
     8941 ggaaccgaag gccgacctcg ccgctcacca tgacgccgga gggcgagccc tccacgggca
     9001 acgtctgcat tcccgggaag agatccggca cggtgccaat ggcgagcgtg acggcgagtc
     9061 ccacctccaa aggtgatttt gcggcggctc cttggtcggt gatcgcgtag tcgatgtcgt
     9121 tgaacgtgat gcgctgaaag tggggtgtct gcgtcgtgct gagcggtggg tcttcgctgg
     9181 cgtcttccgc aacctggact tgctccatat gcactcgggt cggctcacgc ccgaggacgg
     9241 cgaacgcctg cctgatcggc tcgtcggcac ctcgcatcaa caccgcggcg cgcgagagat
     9301 cgagctcggc gaggagcggt gccatttgat cgatcgtgag cgcctggact gcgcgcgcgg
     9361 tggcaaggtc cgacatcggc gcacggtcgt aggcgacgtc ggtctcggcg cgcgcggcga
     9421 tcagggacgc gctacccgtc accgatgcga ggtgcgcgac gacgcgagcg cgcgcaagca
     9481 cgaaggctcg tgcggcggca tcggggtcct tgcgcagctg ttcgatgcgg tcacgtaaca
     9541 acgcgacgac atcgttggcg cgcccgggtt cgacccagcc gccgatgatc catcgcgcgc
     9601 cgagccgatt ctcgtcgagg cccgcgtcga agtcataggt cgcaccgagc tgatggcgga
     9661 cgtcacccgt gatctcagcg agcatcgcgg cggcgacgag ccgctgagcg cgcgtgcccg
     9721 ttgtgaccgg caccgcggcg aggacgagca actgcgtcag attttcgatc tgagcgatcg
     9781 atgccgggtg cggcgttggc ggagtcaggt ggcgtggctg cgcgtgacca tgccaatcag
     9841 cgaacaagaa gtcgatccac ttgttcgcga gcgcgatatc gaagtggccg gcgatcacga
     9901 gcgtcgcgtt gtcgggcgtg aagtacgctg cgcgaaatcg ttgagcgtcc tcgaccgtgg
     9961 catgcggcgt ccc
//
LOCUS       DASUFX010000414         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_66022,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000414 DASUFX010000000
VERSION     DASUFX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66022"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFV99_16580"
     CDS             <1..468
                     /locus_tag="VFV99_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5060986.1"
                     /translation="SVHGDSLVLHPTVNLGIAVDLGAEGLVAPVIKNADRMSVAQLAD
                     AIAEIAQRSRAGKLKPDDFQGGTYTLSNSGTFGTLITAPIINQPQVAILSTDGVRKKP
                     VVVETSEGDSIAIRPVGVLAQSFDHRAVDGAYSAAFLDRVRKIIETSHWVVEV"
     gene            478..1413
                     /locus_tag="VFV99_16585"
     CDS             478..1413
                     /locus_tag="VFV99_16585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5060987.1"
                     /translation="MAKHTLGWVGAGGRMGFAMAKRLLAAGNKVSVYNRTRSKVEPLA
                     ALGATIVDSPRDLRDHDIVFTTVSASDDLIAVCTGPGGLLDKSGSPKLIIDCSSVSEE
                     ASAQVRAAAAELGTEMLAAPVSGNAKVVEAGKLTIVASGPRKSFDMAEPYLQALGLGV
                     TYVGEGELARMVKICHNLLLGIVAQSLAEITVLAEKGGVKRSAFLEFINNSVMGSQFS
                     RYKTPALVNLDWKPTFTPVLLRKDLDLGLKAAKELGVPLPVTKVARGEVDKLVQAGHT
                     ECDFAILIEQAATAAGMKLTPENVAVDDGLKKKSA"
     gene            1873..2496
                     /locus_tag="VFV99_16590"
     CDS             1873..2496
                     /locus_tag="VFV99_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060988.1"
                     /translation="MFASIWVNERKIASRVRFAGYCRDQRQPVISFIVDAARAEPTVR
                     RCVRSNDASSPACEEQPTLTSYPVDRLEYPPTDAERPAVGTYEVLVGEHAVCEAQRRF
                     LQSDQDWRAMADLGLIREQPRQGFDGWTTSTVAVPGQSGQTRASAFDFDNDGKLDRVL
                     DVSFFSTYMISGALLVPAGRKSLRRRRPQEELRLRSSRFSRCGAHRD"
BASE COUNT          474 a          913 c          871 g          457 t
ORIGIN      
        1 agcgtgcacg gcgattcgct cgtgctgcac ccgaccgtca atttgggtat tgcggtcgat
       61 ctcggcgccg aaggcctcgt cgcgcccgtc atcaagaacg ccgaccgcat gagcgtcgcg
      121 cagctcgccg atgcgattgc cgagatcgcg cagcgctcgc gcgccggtaa gctcaagccc
      181 gacgacttcc agggcggcac ctacacgctg tcgaactcgg gcacattcgg cacgctgatc
      241 acggcaccga tcatcaatca gccgcaggtc gcgattctgt cgaccgacgg cgtgcgcaag
      301 aagcccgtcg tcgtcgagac gagcgaaggc gattcgatcg cgatccggcc cgtcggcgtg
      361 ctcgcgcagt cgttcgacca tcgcgccgtc gacggcgcgt actcggccgc gttcttggat
      421 cgcgtgcgca agatcatcga aacgagtcac tgggtcgtcg aagtctgagg ttttggtatg
      481 gcgaagcaca cgctcggctg ggtgggcgca ggcgggcgca tgggcttcgc gatggcgaag
      541 cggctgctcg cggcgggtaa caaggtcagc gtctacaacc gcacgcgcag caaggtcgag
      601 ccgctcgccg cgctcggcgc gacgatcgtc gattcaccgc gggatctccg cgatcacgac
      661 atcgtcttca cgacggtgtc ggcgtcggat gacttgatcg ccgtttgcac cggtcccggc
      721 ggcctgctcg acaagagcgg ttcgccgaag ctcatcatcg attgctcgag cgtttcggaa
      781 gaagcctccg cacaggtgcg cgccgctgcg gcggagctgg gcaccgagat gctcgcggcc
      841 ccggtcagcg gcaacgcgaa ggtcgtcgag gccggcaagc tcacgatcgt cgcctcgggc
      901 ccgcgcaagt cgttcgacat ggcggagccg tatctgcaag cgcttgggct gggcgttacc
      961 tatgtcggcg aaggcgagct cgcgcgcatg gtcaagatct gccacaacct gctgctcggc
     1021 atcgtcgcgc agtcgctcgc cgagatcacc gtgctcgccg agaaaggcgg cgtgaagcgc
     1081 agcgcgttcc tcgagttcat caacaacagc gtcatgggct cgcagttctc gcgctacaag
     1141 acgccggcgc tcgtgaatct cgattggaag ccgacgttca caccggtgct gctgcgcaag
     1201 gatctcgacc tgggcctcaa ggccgcgaaa gagctcggcg tgccgctgcc cgtcacgaag
     1261 gtggcgcgcg gcgaggtcga caagctcgtg caggccggcc acacggagtg cgacttcgcg
     1321 atcttgatcg agcaggccgc gaccgcggcc ggcatgaagc tcacgcccga gaacgtcgcc
     1381 gtcgacgacg gcctcaagaa gaaatccgct tagcgaccct ggcccacgcg gcgagcgtcc
     1441 tcggcacgag accgtcgtct cactgcgcac tccgcgcagt gttcgtcggt gctgctcgca
     1501 ggtagcatct gaacgagccc caaatggacg ccggtcccca tgtcccgagc cgcgggcaca
     1561 gccatcttct ttctagcgat gctcgcagcg agcccgcttc aagcgcagga gcctggggcc
     1621 tttcgcgcgg tcaggctcgc gtgccactac gtcgcggcta ccgactccgc tatggccacc
     1681 atcgaacgcg ttgccgatgg cccgttcgag cgagcagcca ctcgagtact cgatgggtca
     1741 tcctcgagcg acgcggtgga ctttttcgtc gggccaggag agcacgccga gtgcgcgttt
     1801 ccagccgggg cgcgcgttcg catcaaagtc ggtcacgatg acgccggctg ccgtaacaac
     1861 cccgacggcg aggtgttcgc gtcgatctgg gtgaacgaaa gaaagatcgc ctcacgcgtc
     1921 cgatttgccg gctactgtcg cgaccaacgc cagccagtca tcagcttcat tgtcgatgca
     1981 gcgcgcgcag aaccgacggt tcgccggtgc gtaaggtcga acgatgcatc gtccccggca
     2041 tgcgaggagc aacccacgct gacgagctat ccggtcgatc gcctcgaata cccgccgacc
     2101 gatgccgaac gccccgcggt tggcacctac gaagttctcg tgggcgagca tgcagtctgc
     2161 gaagcgcaac gccgcttctt gcagtccgac caagattggc gcgcgatggc cgacctcggt
     2221 ctgattcgcg agcagccacg gcaggggttc gacggctgga cgacgagtac cgtcgccgtt
     2281 cccggacagt ccgggcaaac ccgcgcgagc gcgttcgact ttgacaatga cggcaagctg
     2341 gaccgcgtgc tcgatgtgtc gttcttctcg acgtacatga tctccggggc cttactggtt
     2401 ccagccggcc gcaagtcact ccgtcgacga cggcctcaag aagaactccg cttaagatcc
     2461 tcgcgcttca gtcgttgcgg cgctcaccgc gactagcgcc gacgatctgc cgtactggcc
     2521 gccgcccttt cacgatacta tccacggcgg tccggcgcag gcgctggatt tgggacgacg
     2581 agcccgtgac gtccgacaac gaccgccgct ttgagggtac cgagatccgg cccgtcgcga
     2641 ccgacgcaga cgccatcgca cccggcacga ccatcggtca ttaccgcatc gacacgcttc
     2701 tcggtgccgg cggga
//
LOCUS       DASUFX010000415        18527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1039970, whole genome shotgun sequence.
ACCESSION   DASUFX010000415 DASUFX010000000
VERSION     DASUFX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18527
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039970"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFV99_16595"
     CDS             <1..462
                     /locus_tag="VFV99_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060989.1"
                     /translation="GAWMSRLQEGTFALSVGYSLDGPTPYQFYRWTMSSKTVLPVGTV
                     SPGNWHRLGDRQADALLGELDHASSPEVQHSVTVALERRFAMVAPAIPLFANPLWGEF
                     STAHFVGFPDADHPFARPSPNAEPDALLVLTAIEPRTDDEHTSLASIGGAQ"
     gene            468..1442
                     /locus_tag="VFV99_16600"
     CDS             468..1442
                     /locus_tag="VFV99_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5060990.1"
                     /translation="MALSRLAFYVVAAWAALTLNFLLPRLMPGDPTTAMVGRLSASAS
                     PESIAALRETFGHTDAPLYRQYGAYLVDLAHGDLGRSLSQYPAPVSDVIATGLVWTLG
                     LGGMAVLISFVLGTALGALAAWRRSGWLDAVLPPVLALSGAFPYFWLAMLALHLFGFT
                     LGWSPLRHAYCDHLTAAPTWRFALDVIQHATLPALAIVIATVGGWLLGMRNAMIGVLG
                     TEQITLARAKGLSNRRILWAYAARNAILPNLTSFGMALGFVLGGSLLTELVFSYPGTG
                     YLLIQAVRTQDYPLMQGLFLAITMAVLAANFLVDLVTLALDPRTRPHA"
     gene            1435..3879
                     /locus_tag="VFV99_16605"
     CDS             1435..3879
                     /locus_tag="VFV99_16605"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5060991.1"
                     /translation="MRELMRALYGNRKALVGAAVLVLFALVAIVGPFVFPDAGAPVGR
                     PLAPPSWQHWLGTTGQGQDVLALTVTGARNTLAIAFAVGALVVLLGALVGVTAGYFGG
                     SVDRALSLGTNVFLVIPGLPLAIVIAAYLRPGTASVIAVLALSGWAWHARVFRAQALT
                     LRARDYIAAARITGESHARVIVGELLPNMTALLAAAFIGATIYALGAIVGLEFLGVGD
                     PGAVSWGANLYWAANDAALLTGSWWVFVPTGVCIALVGFALTMLNVALDEFTNPRLRP
                     CAAHEPAAAPATTTTSTPEHDDGVIAVRDLRVTFGETRAVDDVSFDLLPGEILGLAGE
                     SGSGKSTIGYALLRLLDESACQCAGHIRFAGGDVLDMNARQLRAYRWADVAMVFQGAL
                     DALNPVLTAGDQITDVLRARAGLDAAAADARARELIGFVGLEPHHLRAYPHQLSGGMR
                     QRVVIAIALALSPKVLILDEPTTSLDVIVQRDILAHIQRLRRELGLTILFISHDLPLL
                     LSIADRVGVMQHGRLLEVDTPAALRAHAKDPYTRRLLSAFPNLEPPARTPVSTDFRPT
                     LLEVSHLSRRYGRARTFALDNVSFDLRAGEVLAVVGASGGGKSTLARLLTGLDRPDRG
                     TVRVAGNGAHRQHVQMVFQDPFASLNPARRIRHHLARPLRIHGGPQDLDQRIESLLAT
                     VGLPPAFARRFPHELSGGQRQRVALARALAADPRLLIADEPTSMLDASLRAELLALLR
                     ELAHERGLGVVLVTHDLPSAAAIADRVLVLRRGRVVEDGPIGPTLRAPSHPYTGELLD
                     AARHCPSSLERHEAVS"
     gene            3863..5263
                     /locus_tag="VFV99_16610"
     CDS             3863..5263
                     /locus_tag="VFV99_16610"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX5060992.1"
                     /translation="MKRFPDNFVWGAATSAYQIEGAVREDGRGESIWDRFTAEPGRIS
                     DGSDASVACDHYHRVDEDLDVLSWLGVRSYRFSIAWPRVLPTGHGTVNTPGLDFYDRL
                     VDRLLARDIEPFVTLNHWDLPQALHDAGGWPARDTVNAFVRYAEIVMRRLGDRVRRVC
                     THNEPWCISALGFGRGEHAPGEQSWPRALAAAHHLLLSHGRAVQAIRAISRATQLGIV
                     LNLVPAEPASPSEHDRDACRAFDGSFNRWFLDPLYDRGYPLDVIDDHVRAGHLQTHDL
                     PFVHDGDLRTIATATDFLGINYYSRAVMRSTTVPEHENAPPTVVADGERTDMGWEVAP
                     DGLLAILRRVHTEYAPPRLYITENGAAYGTEPDARGRVRDVSRQIYLWSHFEAAHTAI
                     AEGIPLAGYYLWSLLDNFEWGEGYSKRFGLFWVDYQTQARLPKDSAHLCRHIIRGNTL
                     TSLEHDLARVGGSEQP"
     gene            5269..8373
                     /locus_tag="VFV99_16615"
     CDS             5269..8373
                     /locus_tag="VFV99_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060993.1"
                     /translation="MRALWAIAIVAATAGVAHATEAHVVHDDRGYKLQVDGKDLMVRG
                     MNWGYTPIGSNYSYSLWAQSDEFVERVLHRDMEFLRGMGVNMIRQGPDIPPRWVEWIH
                     THYGIYTMINHTMGRYGATIDGVWIAQIDYADQHQRAALVADIVAVVERYRGTRGVAL
                     WLLGNENNYGLAWTSFEAENLPKGQEDAARATALYSLYGEVIRAIKQRDSSHPVAIAN
                     GDLQYIDLIATQCKGLDILGSNVYRGASARDFFQVVEDKLHVPAMFTEFGADAYDAHA
                     DREDATAQAEYLRKQWQEIYEQSWGKGKVGNAIGGFIFQWSDGWWKHGQEINLDVHDT
                     TASWSNDAYPKDYVAGENNMNEEWFGIAAIEDQDADGFYQVQPRVAYYVLREAFRLDP
                     YADTTTPEQIKTHFDEVHEGELSAPYEGLHARATADKLSRIRVSGLRMRLESNVTNAS
                     AMSDRANAQRFDHTESLFVDVTVQPTPKVMSKATINLVGNAAQNRLDPLYWENRTPRP
                     SSDAMTPAMPAADDMLSSDHVSLYGAELHADLPVVGIEGFYRVGHGHWGYEGDFFGLY
                     REAYYGIAIDTYHATAPLGAVLSGKGALGDIKVALGPELYWGANPSVIGKWSHAFGAV
                     TLTALHQEDVAAHSGAATSSAAFQPVTRRSTLQAQIKRGSAVLDVGGIFAASQRVGRS
                     YTFAKPSANAGYLDSGYDVYTGRIAWADTLGGRARLAYDGGRARWYVEGGYRGLVADA
                     GSDQTTTFTGWSMKSSGRGNQMSGLAGALLTFDSFQVAPNLLYQRPIIGPSPAIPDRY
                     DQATGMYYPGVSPRNALADPFVVLDNRETQGMELLLVYDPTPATWYWNWDRDNREDAR
                     FATSLDVVYRHQPTSRDATLVILADGSQVPSAATPPAHDVWSARLAWSSAAALPLRLS
                     GSFYTGQDQANAGDPRLVTRFGGSLRMVRAGLMASTDIKIRDWGPYDYHRDFNLTYPF
                     QWYGDLSYGLPRSALGIGDARFGARWQLRFLDGFSEGYVGDPALSRKLATESEVLTYL
                     EVRL"
     gene            8370..8918
                     /locus_tag="VFV99_16620"
     CDS             8370..8918
                     /locus_tag="VFV99_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060994.1"
                     /translation="MIDRHLLGLLALTASACATDGVPWQGQPLSDEPLVFSSPDEGVH
                     PSRAVLDDPANPFVSGKLTDAAIWQLQSSAGNVSAFYAWATALAYGATGERQYYAALD
                     LKAILEREQATDLPAVRDRAIRGFQAVLTYFPKAVTYDASGTIAYELATPSVIAIQEL
                     GGTAEGWILVMTPDGHTVAVPR"
     gene            9056..10306
                     /locus_tag="VFV99_16625"
     CDS             9056..10306
                     /locus_tag="VFV99_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5060995.1"
                     /translation="MSSRSSQAEYGIGLVLSGGGVRGAYEAGVLAGIADVLASRPSFD
                     VICGSSVGAINAAHLAAHADRADFGIDKLLSSWRSLRLETHLRPRLSTFLGWGQDAQL
                     RKAEQGSARASRWGRALLDPRPFEELVCTTIPWEGLHRNVHAGVLKALIVSALDIARA
                     RTNTFIELAPGASCVLLPARDPRRTGRIETITSDHVLASAALPLVFPARAIGGSYFCD
                     GGLRFNTPLAPAIRTGARKIVVVTLRGGAASADEVVAHYYPKPIFLIGKVLEALLQDP
                     IDYDLQVVERLNRVLDVMADAVPPEAAERIAKVLEADRGLAYQRVRLLVFRPSQNISV
                     LALEHLRRHGAAKASFFAKLLLRKATSLGAYVEADVLSYILFDGMFASTLIELGRRDV
                     QARAAEVTAFFRSETEKPGVPSVA"
     gene            complement(10251..12752)
                     /locus_tag="VFV99_16630"
     CDS             complement(10251..12752)
                     /locus_tag="VFV99_16630"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5060996.1"
                     /translation="MVPLAVFSIVVAIIVAVALTYAGRAWWGWSLGWVFPLAVWWAAG
                     GTWGLTLAIVLWLGVAIVTGIPGIRRRLLVGPLMRRLAPSFPRMSETERIALEAGTVW
                     WGAELFSGAPDFERIIRFAPKQLSDKEHEFLDGPCTQLCRMLDDYAIRKDGDLAPHVW
                     SFIKRERFMGMIIPEEYGGLGFSALAHSSVITMLASRSAAACVTVMVPNSLGPAELLL
                     RYGTEEQKQYYLPRLAIGDEIPCFALTEPHAGSDAAAIRSRGIVCWGSHDGRQVLGIR
                     LTWDKRYITLAPVATVLGLAFRLFDPDHLLGTMTDLGITCALVPVHTPGVWIGARHDP
                     LSSAFQNGPTRGTDVFVPLDQIIGGPQMAGQGWRMLMECLSAGRSLSLPADAVGAAQV
                     ATRVVGAYASVREQFGRPIGQFEGVEAPLGRIAGTMYWMNAVRVITAGAVDAGERPGV
                     VSAIAKRWSTEALRRVANDAMDVAGGAAICKGRHNLLAPIYETVPIGITVEGANILTR
                     SLIVFGQGAIRCHPYALAELRAAEARDVDAFDRAISGHVNFLLRNVVRSVLLSITGGA
                     IARAPDCGRARRPLRKLTRLSASFAVTADIAMITLGAALKRAENISGRMADALAWMYI
                     TFATVNRYVATRVPDDDALFDWATAEALCQTERALHGVIENLPNRLAAGILRLVAFPF
                     GSRLRSPSDRTTTAAAHSLLEGASGRLRLTTDIYVPSAREVGLGQLEYALELAVIARP
                     VRAKVRRALHTGVLSDDSESQVLDQAVAKGILTSQERALVIKAGAARDDVIEVDVFSP
                     LAFHRELEPPFGAASPPVTQPTERRASRSHSERTR"
     gene            12848..13237
                     /locus_tag="VFV99_16635"
     CDS             12848..13237
                     /locus_tag="VFV99_16635"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5060997.1"
                     /translation="MKKPSPATLTRREREIMDILYRRGRATAAEVLEDMANPPTYSAV
                     RALLRILEDEGHIKHVQEGPRYVYLPAVARNDARKSALSHVVTTFFDGSIEQVVATLV
                     ESSRSKLSKDELERLAQLIEKAKKEGR"
     gene            13239..15179
                     /locus_tag="VFV99_16640"
     CDS             13239..15179
                     /locus_tag="VFV99_16640"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5060998.1"
                     /translation="MTIASIALAAIRAAVILGVALAALRLLARTSAATRRSVLVAAFG
                     IVLALPIATAVLPALHLRGEVAPDTHALVATSAPDTIVEASPVATAPVIVPIAQAAPA
                     ANAPREATSVSPLALVVALWALGAVTVLARLGIGLSRARRIVRSARFVETLDIAGRPV
                     EVRISSAIETPAVTGVLVPVILLPHDAETWTAERRRIVLAHELAHVANHDCLASAVAQ
                     LAVAMHWFDPLVWLAARRLRVERELAADDQVLDDGVLASSYAEHLLALATSQGDRRMP
                     TGALAMAESSQVSVRIRALLAPHTRTPLGRARFALLGAGLALAALVACATPDRETAPA
                     PPVETTSPAPTQAPAPSTTIDPRVQAVVEDEIERMNTEWSPCAAIVLVLDPHTGNVLA
                     AGYRGGTETTAQLAAVRPMVAGSTIKPLVIAAALEDGVIKPTDRFDTSPDPRSTIVDV
                     TPHATLDVGEILAVSSNVGLVRIFDKLGGAKLAAWGTRFHLASIPSTIASGSPGATVA
                     IGNVSSTPLEIAAAFATLANGGVYHAPTFVPGRGDGERVVRAETAATVLALLEGVTGA
                     QGTGKAARVDGVRVAGKTGTVHLGLGKDTSDYYSSFVGTAPLDNPRYVVFVGAETPRN
                     GGTGGQVAAPVFGHLMARLLAR"
     gene            15419..16513
                     /locus_tag="VFV99_16645"
     CDS             15419..16513
                     /locus_tag="VFV99_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5060999.1"
                     /translation="MRALGELDIALYLREDGEEQLQIVSDAVLSHFRRLLEYLGMVAP
                     AANAGAVDAELDLEGSGPARRSSGQISRGAPRDRSDADRWSALEGEIVTLQYGLGSEL
                     KEFERRFSDALKNDRKEQAVRDLNDATSGLMDGVFAVMTSVYECFLGYAEPERMVPGH
                     RDTLGKALAIRRTLAELRRYVREVNRPIQDRATPADVADENYARIVEVVGHFIESDVF
                     YYLRSDTRRDFWQFHETLANGDARSNRLDCEGFDKYLDSLAFVSQRGVLIKHDSDLKT
                     KITGDLERAVKFAALLPDVVCEVIEQAFVKGERLSGLNDQVDAVVLEWSSMTPEERAD
                     PERAVAVGRKLLELVRPPRRAASVEGEDVF"
     gene            complement(16545..17561)
                     /locus_tag="VFV99_16650"
     CDS             complement(16545..17561)
                     /locus_tag="VFV99_16650"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX5061000.1"
                     /translation="MLRIAVCLVAFAGCIDDADVTDDSLVETVSLPEFHGRFGSPTIV
                     RDGDTLHAYFAIQAYEGEAVHVTHARSDDGGATWLRVGEALPRLNTDALQSGAVWAPG
                     AAKIDDHHWMLYYTAQVAGTVHKMCTYRARAAGPHGPFVDDFNAPMICPDSTLWAIDP
                     YPVKDAHGDFHLMARIDEPNGVNTIQIRKLGEYGQHFAPGSDWVKLTQITKGGWEEPV
                     MENAAMVRLDGRWYVFYSGGSYRNNSYGVGYADCGPSINGPCVKKTVAAPWLSSQPKI
                     HMYGPGTPTFYRDGGHTFMAVNTWEYSGGQSNPKNHGQVMHVFEVHIAPNGKPVATFV
                     RMVE"
     gene            complement(17676..>18527)
                     /locus_tag="VFV99_16655"
     CDS             complement(17676..>18527)
                     /locus_tag="VFV99_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061001.1"
                     /translation="VDTSGVFDCTDGASNGWSLTKLLGGSQNREGDSILRFPPGVGVH
                     IGGVALINVHYINPTDATLETDVHIRFDTVPAADIVEEGDILFLYNPLISVPGGGTAR
                     AHWRCPVYQDITIANAQSHMHARGVGYEARVDSEQPFYTTTNWERVPVQSYDGFVVKA
                     GSKLDYYCDYRNVEARNVYQGPRSSDEMCMLIGSYYPADPRTGRCLDETGTGTGGEWI
                     GQGTATCQATLGCLQGASDLHAATDCMVTARPEVSAEASAVLRCMISAHDPQAECGAQ
                     IQACSAI"
BASE COUNT         2972 a         6443 c         5950 g         3162 t
ORIGIN      
        1 ggcgcgtgga tgagccgcct gcaggagggc acgttcgcgc tgtccgtcgg ttactcgctc
       61 gacggcccga cgccgtacca gttctaccgc tggacgatga gcagcaagac cgtgctgccg
      121 gtcggcacgg tctcgcccgg caactggcac cgcctcggcg accgccaagc cgatgcactg
      181 ctcggcgagc tcgaccacgc gtcatcgccg gaggtgcagc acagcgtcac cgtcgcgctc
      241 gagcgccggt tcgcgatggt cgcacccgcg attccactgt tcgcgaatcc gctgtggggc
      301 gaattctcga cggcgcactt cgtcggcttt ccggatgccg atcatccgtt cgcgcgaccg
      361 tcgccgaacg ccgagcccga cgcgctgctc gtactcaccg cgatcgagcc gcgcaccgac
      421 gacgagcaca cgagcctggc cagcatcgga ggcgcgcaat gatccgcatg gcgctgtcgc
      481 ggctcgcgtt ctatgtcgtg gcggcgtggg ccgcgctcac gctcaacttc ttgttgccgc
      541 ggctcatgcc aggcgatccg acgacggcga tggtcggccg actctcggca tcggcgtcgc
      601 ccgaatcgat cgccgccctg cgcgagacgt tcggccacac agacgccccg ctctatcgcc
      661 agtacggtgc gtacctcgtc gatctcgcgc acggcgatct cggccgctcg ctctcgcagt
      721 acccggcacc ggtcagcgac gtgatcgcga ccgggctcgt ctggacgctc ggcctcggtg
      781 ggatggccgt cctcatcagc ttcgtgctcg gcaccgcgct cggcgcgctc gccgcgtggc
      841 ggcgctcggg ctggctcgac gccgtactgc ctccggtgct cgcgctgtcc ggtgcgtttc
      901 cgtacttctg gctcgccatg ctcgcgctcc acctgttcgg cttcacgctc ggctggagcc
      961 cactacgcca cgcgtattgc gatcacttga ctgccgcgcc gacgtggcga ttcgcgctcg
     1021 acgtgatcca gcacgcaaca ctgccggcgc tcgccatcgt gatcgccacc gtcggcggct
     1081 ggttgctcgg catgcgcaac gcgatgatcg gcgtgctcgg taccgagcag atcacgctcg
     1141 cgcgcgcgaa gggcctgtcg aaccgccgca tcttgtgggc gtacgctgca cgcaacgcga
     1201 tcctgccgaa cctcacctcg ttcggcatgg cgctcggctt cgtgcttggc ggctcgctgc
     1261 tcaccgagct cgtgttctcg taccccggca ccggctacct gctgatccag gcggtgcgca
     1321 cgcaggacta cccgctgatg cagggcctgt ttctggcgat cacgatggcg gtgctcgccg
     1381 caaacttcct ggtcgacctc gtcacgctcg cactcgatcc gaggacgcgc ccccatgcgt
     1441 gagctcatgc gcgcgctcta cggcaaccgc aaggcgctcg tcggcgccgc ggtgctcgtg
     1501 ctgtttgcgc tcgtcgcgat cgtgggtccg ttcgtgtttc ccgacgcggg cgccccggtc
     1561 ggtcggccac tcgcgccacc ctcgtggcag cactggctcg gcaccaccgg ccaaggccag
     1621 gacgtgctgg cgctcaccgt gaccggcgcg cgcaacacgc tcgcgatcgc gttcgcggtc
     1681 ggtgcgctgg tggtgctgct cggtgcgctt gtcggcgtca ccgccggtta cttcggcggc
     1741 agcgtcgatc gcgcgctgtc actcggcacc aacgtgtttc tcgtgattcc gggattgccg
     1801 ctcgcgatcg tcatcgctgc atacctccgc cccggcaccg ccagcgtgat cgccgtgctc
     1861 gcgctctcgg ggtgggcgtg gcacgcgcgc gtattccgcg cccaggcgct cacgctgcgt
     1921 gcgcgcgatt acatcgcggc cgcgaggatc accggcgagt cacacgcccg cgtgatcgtc
     1981 ggcgagctgt tgcccaacat gacggcgctg ctcgccgccg ccttcatcgg cgcgacgatc
     2041 tacgcgctcg gtgcgatcgt cggcctcgag ttcctcggcg tcggcgatcc gggtgcggtg
     2101 agctggggcg cgaatcttta ctgggcggcg aacgacgccg cgctcctgac cggttcgtgg
     2161 tgggtgttcg taccgaccgg cgtgtgcatc gcgctcgtcg gcttcgcact gaccatgctc
     2221 aacgtcgcgc tcgacgaatt cacgaacccg cgcctgcgtc cgtgcgccgc acacgagccg
     2281 gccgccgcgc cggcaacgac gacaacgtcg acaccagagc acgacgacgg cgtgatcgct
     2341 gtacgcgacc tgcgtgtcac gttcggcgag acgcgcgcgg tcgatgacgt ctcgttcgat
     2401 ctgttgcccg gcgagatcct cggcttggct ggcgaatcgg ggagcggcaa gtcgacgatc
     2461 ggctacgcgc tgctccggct gctcgacgaa tcggcgtgcc agtgcgcggg gcacattcgc
     2521 ttcgctggcg gcgacgtcct cgacatgaac gcgcgccagc tgcgcgccta tcgctgggcc
     2581 gacgtcgcga tggtgttcca gggcgcgctc gacgcgctca accccgtgct cactgccggc
     2641 gatcagatca ccgacgtgct gcgcgcccgt gccggcctcg atgccgctgc cgccgacgcg
     2701 cgcgctcgcg agctgatcgg cttcgtcggc ctcgagccgc atcacttgcg cgcgtatccc
     2761 caccagctct cgggcgggat gcgccaacgc gtggtcatcg cgatcgcgct cgcgctatcg
     2821 ccgaaggtcc tgatcctcga cgagccgacg acgtcgctcg acgtgatcgt gcagcgcgac
     2881 atcctcgctc acatccagcg gctgcgacgc gagctcggcc tgaccatcct gttcatcagt
     2941 cacgatctgc cgctgttgct ctcgatcgca gaccgggtcg gcgtgatgca gcacggccgg
     3001 ctgctcgagg tcgacacgcc cgccgcgctg cgcgcccacg ccaaggatcc ctacacccgc
     3061 cggctgctga gcgcgttccc gaacctcgag ccgccggcgc gtacgccggt gtccaccgac
     3121 ttccgaccga cgctgctcga ggtgagccat ctgtcgcggc gctacggacg cgccagaacg
     3181 ttcgcgctcg acaatgtctc gttcgacctt cgcgccggtg aggtgcttgc ggtcgtcggc
     3241 gcctcgggcg gcggcaagag cacgctcgcg cgcctgctca ccggtctcga tcggccggat
     3301 cgcggcaccg tgcgagtggc cggcaatggc gcgcaccgcc aacacgtcca gatggtgttt
     3361 caagatccgt tcgcgtcgct caaccctgcg cgtcgcatcc gccaccacct ggcgcgcccg
     3421 ctccgcatcc acggcgggcc tcaggatctc gatcagcgca tcgagtcgct gcttgccacc
     3481 gtcggcctgc cacccgcatt cgctcgccgc ttcccgcacg agctctcggg tggccagcgc
     3541 cagcgcgtcg cgttggcgcg cgctctcgct gcggatcctc gcctcctcat cgccgacgag
     3601 ccgacctcga tgctcgacgc gtcgctgcgt gccgagctac tcgccttgct gcgcgagctc
     3661 gcccacgagc gcggtctcgg cgtcgtgctc gtcactcacg atcttcccag cgcggcggcg
     3721 atcgccgacc gcgtattggt cctgcgccgc ggccgcgttg tcgaggatgg cccgatcggt
     3781 ccgacgctgc gcgcgccgtc gcacccctac accggcgagc tgctcgatgc tgctcgccac
     3841 tgcccatcgt ccttggagcg ccatgaagcg gtttcctgac aacttcgttt ggggtgcagc
     3901 cacctccgcg tatcaaatcg agggtgccgt ccgcgaagac ggccgcggcg agtcgatctg
     3961 ggatcgattc accgccgagc caggccggat cagcgacggc tccgacgcca gcgttgcgtg
     4021 cgatcactac caccgcgtcg acgaggacct cgacgtgctg tcgtggctcg gcgtgcgctc
     4081 gtatcgattc tcgatcgctt ggcctcgcgt gctgcctacc ggccacggca cggtcaacac
     4141 gccaggcctc gatttctacg atcgtctcgt cgaccggctg ctcgcacgcg acatcgagcc
     4201 attcgtcacg ctgaaccact gggatctgcc gcaggcgctg cacgatgccg gcggctggcc
     4261 cgcgcgcgac accgtcaacg cattcgtccg ctacgcggag atcgtgatgc gccgcctcgg
     4321 cgatcgcgtg cgccgggtgt gcacgcacaa cgagccgtgg tgcatcagcg cgctcggctt
     4381 cgggcgcggc gagcatgcac ccggcgaaca gtcctggccg cgcgcgctcg ctgccgcaca
     4441 tcatcttctc ctctctcacg gccgcgcggt ccaagcgatc cgcgcgatct cgcgggcaac
     4501 gcagctcggc atcgttctca acctcgtgcc tgccgagccg gcatctccgt cggagcacga
     4561 tcgcgatgct tgccgcgcgt tcgacggcag cttcaaccgc tggtttctcg atccgctcta
     4621 tgaccgcggc tacccgctcg acgtgatcga cgaccacgtg cgagccggac atctacagac
     4681 gcatgatctg ccgttcgtgc acgacggcga cttgcgcacg atcgccaccg cgaccgactt
     4741 tctcggcatc aactactact cgcgcgccgt gatgcgctcg accacggtgc ccgagcacga
     4801 gaatgcgcct ccgacagtcg tcgccgacgg cgagcgcacc gacatgggct gggaggtcgc
     4861 gcccgacggg ctgctcgcga tcctgcgccg cgtccacacg gagtacgcac cgccgcggct
     4921 ctacatcacc gagaacggcg ccgcctatgg caccgaaccc gacgccaggg gccgcgtccg
     4981 cgacgtgtcg cgccagatct acctgtggag ccacttcgag gcagcgcaca ccgcgatcgc
     5041 ggaaggcatc ccgctcgccg gctactacct gtggtcgttg ctcgacaact tcgagtgggg
     5101 cgagggctac agcaagcgct tcggcttgtt ctgggtcgac tatcagaccc aagcgcggct
     5161 gccgaaagac tcggcgcatc tgtgtcgcca catcattcgc ggcaacacgc tcacgagcct
     5221 cgagcacgac ctcgcgcggg ttggcgggag cgaacaacca tgacagcgat gagagcgcta
     5281 tgggcaatcg cgatcgtcgc ggcgacggcg ggtgtggcgc atgccaccga ggcgcacgtc
     5341 gtccacgatg accgcggcta caagctgcag gtcgacggca aagacctgat ggtgcgcggc
     5401 atgaactggg gctacacgcc gatcggctcc aactacagct actcgttgtg ggcgcagtcc
     5461 gacgaattcg tcgaacgcgt gctccaccgc gacatggagt tcttgcgcgg catgggcgtc
     5521 aacatgatcc ggcagggccc cgacattccg ccccgctggg tcgagtggat ccacacccac
     5581 tacggcatct acacgatgat caaccacacg atgggacgct acggcgcgac catcgacggc
     5641 gtctggatcg cgcagatcga ttacgccgac cagcatcagc gcgctgcgct cgtcgcggac
     5701 atcgtcgcgg tcgtcgagcg ctaccgtggc acgcgcgggg tcgcgctgtg gctactcggc
     5761 aacgagaaca actacggcct cgcgtggacc agcttcgagg ccgagaactt acccaaaggc
     5821 caagaggacg cggcacgcgc gacggcgctc tactcgctct acggcgaagt gattcgcgcg
     5881 atcaagcagc gcgactcgtc ccaccccgtc gcgatcgcga acggcgatct gcagtacatc
     5941 gatctcatcg cgacgcagtg caagggcctc gacatcctcg gctcgaacgt ctatcgcggc
     6001 gcctcggccc gtgatttctt ccaggtcgtc gaggacaagc tgcacgtgcc ggcgatgttc
     6061 accgagttcg gtgccgacgc gtacgacgcg cacgccgatc gcgaggacgc gacagcgcag
     6121 gccgagtacc tgcgcaagca atggcaggag atctacgagc agagctgggg caagggcaag
     6181 gtcggcaatg cgatcggcgg cttcatcttc cagtggtccg acggttggtg gaagcacggc
     6241 caggagatca acctcgacgt tcacgacacc acggcgtcgt ggtccaacga cgcttatccg
     6301 aaggactacg tcgcgggcga gaacaacatg aacgaggagt ggttcggcat cgcggcgatc
     6361 gaggatcagg acgccgacgg cttctaccag gtgcagccgc gcgtcgcgta ctacgtgctg
     6421 cgcgaggcgt tccgtctcga tccgtacgcc gacaccacga cgccggagca gatcaagacg
     6481 cacttcgacg aggtccacga aggcgagctg tccgcgccct acgagggcct gcacgcgcgc
     6541 gccaccgccg acaagctgtc gcgcatccgg gtctcaggcc tgcgcatgcg cctcgaatcg
     6601 aacgtgacca acgcgagcgc gatgagtgac cgcgccaacg cgcagcgctt cgatcacacc
     6661 gagtcgctgt tcgtcgacgt caccgtgcag ccgacgccga aggtgatgtc gaaggccacg
     6721 atcaatctcg tcggcaacgc agcgcagaac cgactcgatc cgctgtactg ggaaaaccgc
     6781 acgccgcgcc ccagcagcga cgcgatgaca cccgcgatgc ccgctgccga cgacatgctg
     6841 tcgagcgatc acgtctcgct gtacggcgcc gagctccacg cggatctacc tgtcgtcggc
     6901 atcgagggct tctaccgagt cggtcacggc cactggggct acgagggcga cttcttcggg
     6961 ctctatcgcg aggcctacta cggcatcgcg atcgacacct atcacgcgac cgcgccgctc
     7021 ggcgccgtgc tgagcggcaa gggtgcgctt ggcgatatca aggtggcgct cggccccgag
     7081 ctctactggg gcgcgaatcc gtcggtgatc ggcaagtgga gccacgcgtt tggtgcagtc
     7141 acgctcaccg cgttgcacca ggaagatgtc gccgcacaca gcggcgccgc gacctcgagt
     7201 gcagccttcc agccggtcac gcgccgctcg acgctgcaag cgcagatcaa gcgtgggtct
     7261 gcggtgctcg atgtcggcgg catcttcgcc gcatctcagc gcgtcggccg cagttacacg
     7321 ttcgccaaac cgtcggcgaa cgccggctat ctcgacagcg gctacgacgt gtacaccgga
     7381 cgcatcgcgt gggccgacac gctgggtgga cgcgcgcgcc tcgcgtacga cggcggtcgc
     7441 gcgcgctggt acgtcgaggg cggctatcgc gggctggttg ccgacgcggg cagtgatcag
     7501 acgaccacgt tcaccggctg gtcgatgaag tcgtcgggcc gcggcaacca gatgagcggc
     7561 ctcgctggcg cgctgctgac gttcgactcg ttccaggtcg cgcccaatct gctgtaccag
     7621 cggccgatca tcggaccgtc gcccgcgatc cccgatcgct acgatcaggc gaccggcatg
     7681 tattaccctg gtgtgtcgcc gcgcaacgca ctcgctgatc cgtttgtcgt cctcgacaat
     7741 cgcgagacgc aaggcatgga gctcttgctc gtgtacgacc cgacgcccgc gacctggtac
     7801 tggaactggg atcgcgacaa ccgcgaggac gcgcgcttcg cgacgagtct cgacgtggtc
     7861 tatcgccatc aaccgacctc gcgcgatgcg acactcgtga tcctcgccga tggcagccag
     7921 gtcccgagcg cggctactcc accggcgcac gacgtgtgga gcgcgcgcct tgcctggtcg
     7981 tcggcagcag cgctgccgct gcgtctgtcg ggctcgttct acacgggcca ggatcaggcc
     8041 aacgctggcg atccacgttt ggtgacgcgg ttcggtggtt cgctgcgcat ggtgcgcgcc
     8101 ggattgatgg catcgaccga catcaagatc cgcgactggg gtccatacga ctaccaccgc
     8161 gacttcaacc tcacctatcc gttccagtgg tacggcgatc tgtcgtacgg cctgccgcgc
     8221 agtgcgctcg gcatcggcga cgctcggttc ggcgcgcgct ggcagctccg cttcctcgac
     8281 ggtttctccg agggctacgt cggcgatccg gcgctgtccc gcaagctcgc taccgagagc
     8341 gaggtgctca cctatctgga ggtccgatta tgatcgatcg tcatctgctc ggcctcctcg
     8401 cgctgacggc ttccgcctgc gcgaccgacg gcgtgccgtg gcagggccag ccgttgtccg
     8461 acgagccgct cgtcttcagc tcgcccgacg agggcgtgca tccgtcgcgc gccgtgctcg
     8521 acgatccggc caacccattc gtcagtggca agctcaccga tgcggcgatc tggcagctgc
     8581 agtcgagcgc cgggaatgtg tcggcgttct acgcgtgggc gaccgcactt gcgtacggcg
     8641 ccaccggcga gcgtcagtac tacgccgcgc tcgatctcaa ggcgatcctc gagcgcgagc
     8701 aggccaccga tctgcccgcc gtgcgcgacc gcgcgatccg cggctttcag gcggtgctca
     8761 cctacttccc caaggccgtc acctacgacg caagcggcac catcgcctac gagctcgcga
     8821 cgccatcggt gatcgccatc caagagctgg gtggaacggc cgagggttgg atcttggtga
     8881 tgacaccgga tggtcacacc gtcgccgtgc cgcgctgatc gcggtcgacg gttcgtacga
     8941 ctctccgcgg cgcgcgtatt gcacacgacg gcgaagtgaa gagcgcccgc ccggcgcaag
     9001 gatccggtca tgtgcacatc gcgcgtttgg attgcctcgc gtccggagac agcggttgag
     9061 tagtcgctcg tcgcaggccg agtacgggat tggcctcgtt ctctccggag ggggcgtgcg
     9121 cggcgcatac gaggcggggg tccttgctgg catcgccgac gtgctcgcct cgcgcccctc
     9181 attcgacgtg atctgcggta gctcggtcgg cgcgatcaat gccgcccacc tcgcggcgca
     9241 tgcggatcgc gctgacttcg gtatcgacaa gctactgagc agctggcgct cgctccgtct
     9301 cgagacgcac ctgcgtccac gcctgagtac gttccttggt tggggccagg atgcgcagct
     9361 tcgtaaggcc gagcaaggct ccgcccgtgc gtcgcgatgg gggcgcgcgc tgctcgaccc
     9421 gcgcccgttc gaagagctcg tgtgcaccac gattccctgg gagggcctac accgcaacgt
     9481 gcacgcggga gtgctcaagg cactcatcgt ctctgccctc gacatcgcgc gcgcgaggac
     9541 gaacacgttc atcgagctcg cgcccggtgc atcgtgcgtg ctgctgcccg cgagagatcc
     9601 gcgacgaacc gggaggatcg agaccatcac ctccgatcac gtgctcgcat ccgctgcgct
     9661 gccactcgtg tttccagcac gcgccatcgg tggctcgtac ttctgcgacg gcggtctccg
     9721 gttcaacacg ccgctcgcgc cagcgatccg caccggtgcg cgaaagatcg tcgtggtgac
     9781 gctccgcgga ggtgcggctt cagccgatga agtggtggcg cactactatc cgaagccgat
     9841 ctttctgatc ggaaaggtcc tcgaagcgct gctgcaagat ccgatcgact acgacttgca
     9901 ggtggtcgag cgcctcaatc gtgtcctcga cgtcatggcc gacgctgtcc cacccgaggc
     9961 ggcagagcgc atcgcgaagg tgctcgaggc cgaccgcggt ctcgcgtacc agcgcgtgcg
    10021 tttgctcgtg ttccgcccgt cccagaacat cagcgtcctc gcgctcgagc atctgcgccg
    10081 gcacggcgcg gcgaaagcga gtttcttcgc gaagctcctg ctcaggaaag cgacctcgct
    10141 gggtgcctac gtcgaggccg atgtcctctc gtatatcctc ttcgacggga tgttcgcgag
    10201 cacgctgatc gagcttggcc gacgcgacgt gcaggcgcgg gctgccgaag tcaccgcgtt
    10261 ctttcggagt gagaccgaga agcccggcgt tccgtcggtt gcgtgaccgg cggagacgcc
    10321 gcgccgaacg gcggctcgag ctcgcggtga aacgcgagcg ggctgaagac atcgacctcg
    10381 atcacgtcat cacgggcggc gccagccttg atgacgagcg cgcgctcctg ggaggtcaag
    10441 atcccctttg ccaccgcttg atcgagcacc tgcgattccg agtcgtccga tagcacgccg
    10501 gtatgcaacg cccggcgcac cttcgcgcgc acgggccgcg cgatgactgc gagctccagc
    10561 gcgtattcga gctgaccgag tccgacctcg cgcgcgctgg gaacatagat gtcggtggtc
    10621 agtcggagtc ttccactcgc tccttcgagc agcgagtgtg cggccgccgt cgtcgtccga
    10681 tcgctcggcg atcgcaagcg cgagccgaac ggaaacgcta ccaaccggag aatgcccgcc
    10741 gcgagccggt tcggcaggtt ctcgatgacg ccgtgcagcg cccgttccgt ctggcacaga
    10801 gcctccgcgg tcgcccagtc gaacaacgcg tcgtcgtcgg gaacgcgtgt cgcgacgtag
    10861 cgattcacgg tcgcgaaagt gatgtacatc cacgcaaggg catctgccat gcggcccgag
    10921 atgttctcgg cgcgtttcaa cgcagcaccg agcgtgatca tcgcgatgtc cgcggtcacg
    10981 gcgaagctcg ctgacagcct cgtgagcttc cgcagcggcc tgcgcgcccg gccgcaatcg
    11041 ggcgcgcgcg cgatcgcacc gcccgtgatc gagagcagca cgctgcgcac gacgtttctc
    11101 agcaggaaat tgacgtgacc cgaaatcgcc cgatcgaacg cgtcgacatc gcgagcctcg
    11161 gcggcgcgta gctcggcgag tgcatagggg tgacaccgaa tggcgccttg cccgaacacg
    11221 atcagcgatc gcgtgaggat gttcgcgcct tcgaccgtga tgccgatcgg gacggtctcg
    11281 tagatcggcg cgagcaggtt gtgtcggcct ttgcagatgg ctgcgccgcc ggcaacatcc
    11341 atcgcatcgt tcgcgaccct gcgcagcgct tccgtagacc agcgcttcgc gatcgcggag
    11401 accacgccag ggcgctcgcc cgcgtcgact gcacccgcgg tgatcacgcg gacggcgttc
    11461 atccagtaca tcgtgcccgc aatccggccg agcggcgcct ccacgccctc gaactgcccg
    11521 atcggtcgac cgaactgctc gcgaacgctc gcgtacgccc ccacgacacg cgtcgcgact
    11581 tgcgcggcgc caaccgcatc ggccggcaag ctgagggagc gtcccgccga cagacattcc
    11641 atgagcattc gccagccctg gcccgccatc tgtggtccgc caatgatctg atcgagcgga
    11701 acgaacacgt cggtgcctcg ggttggcccg ttctgaaatg ccgaagacag cggatcatga
    11761 cgggctccga tccacacgcc cggcgtgtgc accgggacga gcgcgcacgt gatcccgagg
    11821 tcggtcatgg tgcccagcag gtggtcgggg tcgaataatc ggaatgccag cccgaggacc
    11881 gtcgcgaccg gcgcgagtgt gatgtagcgc ttgtcccacg tgagccggat gcccagcacc
    11941 tgacgtccat cgtggctgcc ccagcacacg atcccccgcg agcgaatcgc tgcagcatcc
    12001 gatccggcat gcggctccgt gagcgcgaag cacggaatct cgtcgccgat ggcgagcctc
    12061 ggcaagtaat actgcttttg ttcctcggtc ccgtaccgca ggagtaactc cgcaggcccg
    12121 agcgagttcg gcaccatcac ggtcacgcac gcggccgcac tgcgagacgc gagcatcgtg
    12181 atcacggagc tatgcgcgag cgcggagaac cccagcccgc cgtactcctc cgggatgatc
    12241 atgcccatga agcgttcgcg tttgatgaag ctccacacgt gaggcgccag atcgccatcc
    12301 ttgcggatcg cgtagtcgtc gagcatccgg cacagctgcg tgcacggacc gtcgaggaac
    12361 tcgtgctcct tgtcggagag ctgtttcggc gcgaatcgga tgatgcgttc gaagtcggga
    12421 gcgcccgaga acaactccgc tccccaccag accgttccag cttcgagcgc gatccgctcg
    12481 gtctcgctca ttcgcgggaa cgacggtgcg agccttcgca tcagcggacc gacgagaagt
    12541 cgccggcgta tcccggggat gccggtcacg atcgcgactc cgagccacaa cacgatcgcg
    12601 agtgtcaggc cccaggtgcc gcctgcggcc caccacaccg ctaggggaaa gacccatccg
    12661 aggctccatc cccaccaggc tctgccggcg tacgtcagcg cgacggcaac aatgatcgcc
    12721 acgactatcg agaacactgc cagcggcacc atgcactgga gagcttgcaa ccggcgcgcc
    12781 tgctggctac gcaggtttcg gatctgattg ttgctagttt tttagcatac tgctaaattc
    12841 ttagcacatg aagaagccat ccccggccac gctcacgcgg cgcgagcgcg agatcatgga
    12901 catcctgtat cggcgtggtc gagcgactgc ggccgaggtc ctcgaggaca tggcgaaccc
    12961 accgacgtat tcggcggtgc gtgcactgct gcgcatcctc gaagacgagg gacacatcaa
    13021 gcacgtccag gaggggccgc gctacgtgta cctgcccgcg gtcgcgcgta acgatgcgcg
    13081 caagagcgcg ctctcgcatg ttgtcacgac gttcttcgac ggttcgatcg aacaagtggt
    13141 cgccacgctc gtcgagtcgt cacgctcgaa gctgtcgaag gacgagctcg agcgcctcgc
    13201 tcagctgatc gagaaggcaa agaaggaggg gcgctaacat gacgatcgca tccatcgctc
    13261 ttgctgccat ccgggcggcc gtgatcttgg gcgtcgccct cgcggcgctg cggcttctcg
    13321 cccggacgtc ggcggcaacg cgccgctcgg tgctcgtcgc tgcgttcggg atcgtgctcg
    13381 cgctaccgat cgcgactgcc gtattgccag cgctccatct gcgcggcgag gtcgcccccg
    13441 atacgcatgc actggtcgcg acctccgcgc ccgatacgat cgtggaagcg agcccggtag
    13501 caaccgcgcc ggtaatcgta ccgatcgcac aggcggcacc cgcggcgaat gcgccgcgcg
    13561 aggctacgag cgtgtcaccg ctcgcgctcg tcgttgcgct atgggcactc ggtgccgtca
    13621 cggtgctcgc gcgtctcggc atcggtcttt cgcgcgcgcg tcgcatcgtg agatcggcgc
    13681 gcttcgtcga gaccctcgac attgccgggc gccccgtcga ggttcgcatc agctcggcca
    13741 tcgagacgcc tgctgtgacc ggtgtgctcg tgccggtgat cctcctgcct cacgacgccg
    13801 agacctggac cgccgagcgc cgccgcatcg ttctcgcgca cgagctcgcc cacgtcgcga
    13861 accacgactg tctcgcaagt gcggtcgcgc agctcgcggt cgcgatgcac tggttcgatc
    13921 cactcgtgtg gctagcggcc cgcaggctgc gtgtcgagcg cgagctcgcc gcggacgatc
    13981 aggtgctgga cgatggtgtg ctcgcgtcga gctacgccga gcacctgctc gcgctcgcaa
    14041 catcacaagg cgatcgccgg atgcccacgg gcgcgctcgc gatggcggaa tcctcgcagg
    14101 tatcggtccg cattcgcgcg ctcctcgcac ctcacacgcg aacgccgctc gggcgcgctc
    14161 gcttcgcact gctcggcgcg ggtctggctc tggcagcgct cgtcgcctgc gccacgcccg
    14221 atcgcgagac cgcaccggcc ccaccggtcg agacaacctc gcccgcgccg acccaagcgc
    14281 cagcgccgtc cacgacgatc gacccgcgag tccaggcggt cgtcgaggac gagatcgaac
    14341 gcatgaacac ggagtggtcg ccgtgcgcgg cgatcgtcct cgtgctcgat ccgcacaccg
    14401 gcaatgttct cgcggccggt tatcgcggtg gcaccgaaac gactgcgcag ctcgctgcag
    14461 tccgcccgat ggtagcgggc tcgacgatca agcccctcgt gatcgcagca gcactcgagg
    14521 acggcgtcat caaaccaacc gatcggttcg atacatcgcc cgatccacgc tccacgatcg
    14581 tggacgtcac gccgcacgct acgctcgacg tcggcgaaat actcgcggtg tcgtcgaacg
    14641 tcgggctcgt caggatcttc gacaagctgg gtggcgcaaa gctcgccgca tggggaacgc
    14701 gcttccacct cgcgtccatc ccgtcgacga tcgccagcgg ttcgccgggc gcgacggtcg
    14761 cgatcggaaa cgtgtcgtcg actccgctcg agatcgccgc agcattcgcc acgctcgcaa
    14821 acggcggtgt ctatcacgcg ccgacattcg ttcccgggcg cggcgatggc gagcgcgtcg
    14881 tccgcgccga gaccgcagcg accgttctcg cgctgctcga gggtgtcacc ggcgcgcaag
    14941 gaacgggcaa ggctgcgcgg gtcgacggcg tccgtgtcgc gggcaaaacg ggcaccgtgc
    15001 acctcggtct tggcaaggac acatccgact actactcgag cttcgtcggc accgctccgc
    15061 tcgacaaccc gcgctacgtc gtcttcgtcg gcgccgaaac tccgcgcaat ggcggaacgg
    15121 gcggtcaggt ggcagcgccg gtgttcggcc acctgatggc gcgcctgctg gcgcgatagg
    15181 tcaaaactca ctctccaagt gcacagaagt tctccgacaa agccctcgat tcgacgggta
    15241 gccgccggat agcggcggga agcgggtagc aaccgcggcg gcaacttcgt atccttagga
    15301 tctggagtcc gaagccgaag cccctgagaa cgcagccctt gccgggctgc tcgccacgat
    15361 ctcggatcgt cgcatccgga gaaaggtcca gaagcttctc cagctcggcg aagaagcggt
    15421 gcgcgcgctc ggcgagctcg atatcgccct gtacttacgg gaagacggtg aagagcagct
    15481 gcagatcgtc tccgacgcgg tgctgagtca cttccgccgg ctactcgagt acctcggcat
    15541 ggtcgcgcct gccgcgaacg ccggcgcagt ggacgcggag ctcgatctcg aaggctctgg
    15601 ccccgcccgg cgaagctctg gccagatctc ccggggcgcc ccccgcgatc gatcggacgc
    15661 cgacaggtgg agcgcgctcg aaggagagat cgtcaccttg cagtacgggc ttggatccga
    15721 gctcaaggaa ttcgaacgcc ggttctccga cgcgctcaag aacgaccgaa aggaacaggc
    15781 cgttcgcgat ctcaacgacg cgacgagcgg cctcatggac ggcgtcttcg cggtcatgac
    15841 gagcgtgtac gagtgcttcc tcggatacgc cgagcccgag cgcatggtgc cgggccaccg
    15901 cgacacactc ggcaaagcgc tggcgatccg gcgaacgctc gccgagctac gccgctacgt
    15961 acgtgaggtc aatcggccga ttcaggatcg tgcaacgccg gccgacgtgg cggacgagaa
    16021 ctacgcgcgc atcgtcgagg tggtcggtca cttcatcgag agcgacgtgt tctattacct
    16081 gcgctccgat acacgccggg acttctggca gttccacgaa acactcgcga acggcgacgc
    16141 gagatcgaat cgcctcgatt gtgaaggctt cgacaaatac ctcgactcgc tcgcattcgt
    16201 ttcgcagcgc ggcgtgctca tcaagcatga cagcgatctg aagaccaaga tcaccggtga
    16261 tctcgagcga gctgtgaaat tcgccgcgct cttgcccgac gtggtgtgcg aggtgatcga
    16321 gcaggccttc gtgaaaggcg aacgcctgtc cggcctcaac gatcaggtcg atgcagtcgt
    16381 cctcgagtgg tcgtcgatga ctccagagga gcgcgctgac ccggagcgtg ccgtggcggt
    16441 cggacgcaag ttgctcgagc tcgtccgccc accgcgtcgc gccgcgtccg tcgagggcga
    16501 agacgtcttc tagtcggcct cgtcgctaga agcgagctcg ctagtcactc gaccatgcgc
    16561 acgaacgttg cgaccggctt tccgttcggc gcgatgtgca cctcgaacac gtgcatgacc
    16621 tggccgtggt tcttcgggtt cgactgaccg cccgagtact cccacgtgtt gacggccatg
    16681 aatgtgtgac cgccgtcgcg atagaacgtc ggcgtcccgg ggccgtacat gtggatcttg
    16741 ggctggctcg acagccacgg cgcagcaaca gtcttcttga cgcacggccc attgatgctc
    16801 ggaccacagt ccgcgtagcc gacgccgtag ctattgttgc gataggaacc gcccgagtag
    16861 aagacatacc agcggccgtc gaggcggacc atcgccgcgt tctccatcac cggttcctcc
    16921 cagccaccct tcgtgatttg cgtgagcttc acccagtcgc tgccgggtgc gaagtgctgg
    16981 ccgtactcgc cgagcttgcg gatctggatc gtgttgacgc cattcggctc gtcgatgcga
    17041 gccatgaggt ggaagtcgcc gtgcgcatcc ttgaccggat acgggtcgat cgcccatagc
    17101 gtgctgtcgg ggcagatcat cggcgcgttg aaatcgtcga cgaagggtcc gtgcggacct
    17161 gccgcgcgcg cgcggtacgt gcacatctta tggaccgtgc ccgcgacctg cgccgtgtag
    17221 tagagcatcc agtggtggtc gtcgatcttc gctgcgccag gcgcccacac ggcaccgctc
    17281 tggagcgcgt cggtgttgag ccgcggcagt gcttcaccca cacgaagcca ggtggcgccg
    17341 ccgtcgtcgg agcgtgcgtg cgtgacgtgc acggcctcgc cttcgtacgc ctgaatcgcg
    17401 aagtacgcgt gcagcgtatc gccgtcccgc acgatcgtcg gcgagccgaa cctgccgtga
    17461 aactcgggaa gcgacacggt ctcgacgagt gagtcgtcag tgacgtctgc atcatcgatg
    17521 cagccggcga atgcgaccag acagacggca attctcagca tggccgggta cgtcgcaacg
    17581 acggtgccag cgtccaatcc gccgagatcg cagtgctctg cggcggcacc tgtaacgtcc
    17641 ggcgctacag gtgctcacga gggtccgcga accgatcaga ttgcactaca cgcctggatc
    17701 tgcgcgccgc actcggcctg tggatcgtgc gcgctgatca tgcagcgcag caccgccgac
    17761 gcttcagccg acacctcggg cctggcggtg accatgcaat ctgtcgccgc gtgcaagtcg
    17821 ctcgctcctt gcagacaccc gagtgtcgct tggcaggtcg ccgtgccctg cccgatccac
    17881 tcgccgcccg ttcccgtgcc agtctcgtcg aggcaccttc ccgtgcgcgg atcggccggg
    17941 taataggaac cgatcagcat gcacatctcg tcactgctgc gcggaccctg atagacgttg
    18001 cgcgcttcaa cgttgcggta gtcgcagtag tagtcgagct tcgatccggc cttcacgacg
    18061 aagccgtcat aactctgcac tggtactcgc tcccaattcg tcgtcgtgta gaacggctgc
    18121 tcgctgtcga cgcgcgcttc atagccgacg ccccgcgcgt gcatgtgcga ttgcgcgttt
    18181 gcgatcgtga tgtcctgata gaccgggcac cgccagtgcg cgcgcgccgt gccgccaccg
    18241 gggacgctga tcagtgggtt gtagaggaag aggatgtcgc cttcctcgac gatgtccgcg
    18301 gcggggaccg tgtcgaaccg gatgtgcaca tcggtctcga gtgtcgcgtc cgtcggattg
    18361 atgtagtgga cattgatcaa cgcgacgcca ccgatgtgaa cgccgacgcc gggcgggaac
    18421 ctcaggatcg agtcgccctc gcggttctgc gatccgccga gcaacttggt cagcgaccat
    18481 ccattgctcg cgccgtcggt gcagtcgaat acgccggacg tatcgac
//
LOCUS       DASUFX010000416        12235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1596636, whole genome shotgun sequence.
ACCESSION   DASUFX010000416 DASUFX010000000
VERSION     DASUFX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596636"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFV99_16660"
     CDS             <1..610
                     /locus_tag="VFV99_16660"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5061002.1"
                     /translation="ELSNVKHEYIDGVVYARDVGTVEEYVALEELSNVKHEYIDGLIL
                     AMSGGTLEHSRLGVAVIGQLLAQLQGRSCYVFSSDARVQVAGGELITYPDVSVCCGTP
                     REGGQDPLAMQNPTVIIEILSPRTEKYDRTTKFARYQQIESLQEYVLVSQSECRVEVF
                     RQLDDRSWSDAVVAGPGERAALRSIACELDIDELYRMRLTSS"
     gene            complement(691..2016)
                     /locus_tag="VFV99_16665"
     CDS             complement(691..2016)
                     /locus_tag="VFV99_16665"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX5061003.1"
                     /translation="MERFVQQLADVLLAGEWNGDALLSRLQHVFEANRPWMDHLVDLI
                     VEEFPLDGPASLEELREVLLDSYALHEAVRERPLVRGAPPPEMAESPWRVPALATSKE
                     LAVWLGLSVDQLTSLADLRGISSTASDARRHHRVSPTALDQRRRHYRYTWIAKRGGYR
                     LLEAPKPRLRTIQRFVLDDIIAQIPPHTAAHGFRAGHSIASFATPHVDRDVVIRLDLQ
                     AFFTSVTRARVAAIFHSAGYPERVARTLAALCTHRTPPDVLYTTPNRQHDGETHARLR
                     TAHLPQGAPSSGALANLAAFRFDVRVAAFAATIGARYTRYADDIAISGRRELARAAST
                     LIARVGAIAFEEGFALNFRKTRVMTRSAAQRLTGLVVNDKLSVPRAEVERLRAILHNC
                     ARTGPAAQNRDGHPDFRAHLLGRIAWVDSIDAAKGARLHAIFDRIVWST"
     gene            complement(2216..3130)
                     /locus_tag="VFV99_16670"
     CDS             complement(2216..3130)
                     /locus_tag="VFV99_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5061004.1"
                     /translation="MKVIVFGATGNTGRALLDQGAAAGHEMTAFVRRPLPPRRVRASA
                     PPSSAPALAHSGAAPNGSPAASIGLTTASTGSTAASTSSSAAFNASAGSFNASAGSFT
                     GSAGSFSGSTAASNGSAAASTGSSAGSTKVSAGSSLPEPRVIIGDVLDLAAVAAAVKG
                     HDAVMSCLGTRPWRHVDICSGGIATIIAAMQSAGMKRIVAMSSLGVGEKTEYRGGFVI
                     RLGGWLVLRKAFRDKLAMERSLAQTDLEWIVVRPGFLTDSKPRGKWRVADDTSLSGGM
                     ISRADTAAFMLQQLTSNEWLRKRPVLVA"
     gene            complement(3213..3632)
                     /locus_tag="VFV99_16675"
     CDS             complement(3213..3632)
                     /locus_tag="VFV99_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061005.1"
                     /translation="MIALRAPQYLSLGIATVATAAIWLTQPTAPALSAPELAVAPPSE
                     PVEIRTQSTQRACDSETISGIVIRGQFTSFERLNEGFTISFAGTVGRYTTMTDAHGYF
                     EVHIPRDDFNADLCQLVSNWKSFSDADMSLKYRLDFE"
     gene            3731..4084
                     /locus_tag="VFV99_16680"
     CDS             3731..4084
                     /locus_tag="VFV99_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX5061006.1"
                     /translation="MRIRLSDEKKPPGPKRDGERQTGLVLDERTRTKKPPMYKVLLHN
                     DDYTTREFVVWVLQTVFHKNESDAIAIMSHVHNHGIGVAGVYTFEVAETKVTKTMSLA
                     KAQQFPLQLSIEPTD"
     gene            4127..6391
                     /gene="clpA"
                     /locus_tag="VFV99_16685"
     CDS             4127..6391
                     /gene="clpA"
                     /locus_tag="VFV99_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX5061007.1"
                     /translation="MLSPELQATLQRAVDDVRGRRHEYLTLEHLLLSCLDDPTGADII
                     TKCGGDVEKLRGDLERFLDEQVEELPDGEESGPDQTLAFQRVLQRAAMHVQAAGRQQM
                     TTGNVIASMFRERDSHAVYLLEKQGITRFDVINYISHGVSKVDAGGPVVPRTRGVEDD
                     GQDDQKISNPLESFCVDLTQRAREGKIDPLIGRADELERMIQVLSRRRKNNPLLIGEP
                     GVGKTALAEGLALRIVDKQVPEPLLDAKVYSLDMTAVLAGTRYRGDFEERLKAVMKVL
                     IGDPKSILFIDEIHNIIGAGATSGGTMDAANILKPPLSNGELKCIGSTTFKDYRQAFE
                     RDRALSRRFQRIDIGEPTIPEAIEILKGLKPRYEEHHNVKFTDAAITAAVELSARHIS
                     GSHLPDKAIDVIDEAGAKVRLMPADQRPDEIRPNLIEDVVAKIARIPTRSVSTQDKTK
                     LANLEGELKRLIFGQDAAIEQLATVIKLSRAGLGNPNKPTGNFLFAGPTGVGKTELAK
                     QLASVLGIEFIRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGQLTDAINKNPYSVVLLD
                     EIEKAHPDIYNILLQIMDHATLTDNNGRKSDFRNVILIMSSNAGSREMAAGRIGFGDS
                     IADSKDGKKALERVFTPEFRNRLDATVFFGSLPVEVIRKVARKFLDELEGQLQEKKVS
                     LEVSPAALDWFVEHGYDKAMGARPMARLVQSTLKAPLANEILFGKLTNGGVAHVDVKD
                     GQIDITCEGGDAPN"
     gene            6467..7216
                     /locus_tag="VFV99_16690"
     CDS             6467..7216
                     /locus_tag="VFV99_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5061008.1"
                     /translation="MAPLIELVDIARYYQMGDERIAALDGVSFAVQAGEMVAIVGTSG
                     SGKSTLLNVLGCLDTPSAGRYRLNGRDVQDLSDDDRARARNQQLGFVFQSFQLLHRAT
                     ALKNVELPLVYRGMPAKERRERAMKALTRVGLSARMKHKPYQLSGGQRQRVAIARALV
                     SEPSLLLCDEPTGNLDSATTEDIMGLFHRLHDEKHTIVIVTHDPSIAARCPRAIRISD
                     GKILADGTGADVARGAATVSVAVPADTVGAA"
     gene            7213..8412
                     /locus_tag="VFV99_16695"
     CDS             7213..8412
                     /locus_tag="VFV99_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061009.1"
                     /translation="MTKLDRLALVALVAACSGGGPRRAGKVEERARVEKVRMAEVRDR
                     VLLAGTVHAIQSEELRTPRTPMWELPVRWMAEDGAVVKKGDRALEFDNSSFVSTLKEQ
                     KLAYLDALMTFDTFLDVSALDEAQKTFELDNAKLTLEKAQVQANVPADLLPARTAQER
                     QLELKRAEAAVEKAQKELASAKAANALERQVKQIELDKAKQKILDAEKAIGELDLKAP
                     RDGIIVVGDHPWMGRKFQIGDTVQPGWTIVELPDLTSGMEVRAELSDVDDGRVMIGMA
                     GTCTLDAYPADGVPCVVKDLMPVASGKGGQSLRRGFAIVLTIDKVDHEKMRPGMAVKV
                     ELAPAKAPAQLVVPRGAVHFDKDKTRVKLASGELRDVELGACDAQGCVVTKGLASGDG
                     VSIGGEL"
     gene            8409..9605
                     /locus_tag="VFV99_16700"
     CDS             8409..9605
                     /locus_tag="VFV99_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5061010.1"
                     /translation="MKRVLLALAAFVALGACNREHGDLALVDVKKADLVVGVEVNGEL
                     AAVDSLDVKPPALYDTWDFKIADLIAEGKEVKEGDPIVAFDPSEQMRTLESLANDADA
                     AKKKLDKKRDDAALARRDEQLQIAQAEATLRKASLKTSADKDLVATVDQKVLEIDGAL
                     AKLALDQAKNKAERAAKSDTAEIKSLADHHEYLAGRVQELQQNIEKMAVKAPRAGTIV
                     YPTNWRGEKKKVGDSCWRMEAVLQIVGLGKMVGNGEIDEIDVARVKDNQPVSIKLDAL
                     PDVQLKGTVQEIAKSVAPKSEADRSNIAKLKIKVDAPATVPLRPGMRFRGEVETERLA
                     NVVQVPADAVFVTPDGPVAYKSTGDGVTKVKLVLGKRNASAIEVKSGLAPGDRVSRID
                     PDRSAP"
     gene            9602..10885
                     /locus_tag="VFV99_16705"
     CDS             9602..10885
                     /locus_tag="VFV99_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5061011.1"
                     /translation="MKKLVVLAVLAAIGGGGWFVVRRSVGAAQHDDVPTHTVKKEAFV
                     RRVTAEGNLRAVKATPVTAPQSGGSFGPMKIAWLAPDGVAVKAGDVVVRFDPTDPEKQ
                     LKEGQADLAAASAKLAEEDIKSKTAVQGRDSAADLAGAELEQQRKFQSKDKEIFSRNT
                     IVEAEIDEKLAGAKQEHAEQTKQIERKLAQSKAAVIAVEKQKAQIAISHAEAALKSME
                     VKAPHDGILVLRRNWRGELPRVGEQTWPGQKIAEIPLLDTMEAEVFVLEVDGNGITEK
                     QPAELVVEAKPENTYKGTIKLVDKLAKPRVPGVPVQYFAVVLELEKTDHAVMKPGQRV
                     RAELILDKADALVVPRQAVIGKEGKNVVYKRGAHGFEPVEVELGAGTSGRVVVTKGLS
                     AGDVIALRDPTRSLDQAIGSGSDGAGEASGGKGKQ"
     gene            10882..12120
                     /locus_tag="VFV99_16710"
     CDS             10882..12120
                     /locus_tag="VFV99_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5061012.1"
                     /translation="MRRVLDAFVVALESLAEHKLRTTLTMLGMMFGVGAVIAMLSIGA
                     GAEKEALALIDRLGTRNVVVRSKTFKPTELEEIRKKSLGLSQRDVEAIEEAVPGVLFA
                     APRIEVEPYKILAAGAKTKAQVYGVSHRHREVTSFALAEGRFLDVNDEKHHGQVCVIG
                     AGVRRDLFGADVAIGRDVKINDLWCEVVGVLAPEPGAVTAVQGVPVSSTEREIYMPFT
                     TALRKLDRDPMKSPLGEIVVRLDPKAPAAETGAVVGTLLDRLHGGARDYDIIVPEALL
                     RHSQQTQQLFNLVMGLIAGISLLVGGIGIMNIMLASVLEQTREIGVRRAVGARRSDIR
                     FQFLVTAFSLAMLGGLAGVAMGLAIARGVASYASWPTVVTTWSIALSLGVSIVVGVIS
                     GLYPAMRAARLDPIQALHAE"
BASE COUNT         2260 a         3859 c         4233 g         1883 t
ORIGIN      
        1 ggagctctcg aacgtcaagc acgagtacat cgacggcgtc gtatacgcga gagatgtcgg
       61 tacggtcgaa gagtacgtag cgctcgagga gctctcgaac gtcaagcacg agtacatcga
      121 cgggctcatc ctcgcgatga gtggcggcac actcgagcac tcacggcttg gtgtcgcggt
      181 gatcgggcag ctcctggctc agctgcaggg gcgcagctgc tacgtgttct cgtccgatgc
      241 gcgcgtacag gtggccggtg gcgagctcat cacgtacccc gacgtctcgg tctgctgcgg
      301 gacacctcgt gagggcggcc aggacccgct cgcgatgcag aacccaacgg tgattatcga
      361 gatcctgagc ccgaggaccg agaagtacga tcgaaccacg aagttcgccc gctatcagca
      421 gatcgaatcg ctgcaagaat atgttctcgt gtcgcagagc gaatgccgcg tcgaggtgtt
      481 ccgacagctc gacgatcgct cgtggagcga tgcagtcgtc gccggtcccg gcgaacgcgc
      541 agcgttgcgt tcgatcgcct gcgagctcga catcgacgag ctctaccgga tgcggttgac
      601 gagcagctga gcagggcaga gctcgcaccc cgacaagacg ggccttccgc agtcatcatg
      661 ggcgtcagct gttggtgcta acgccagcaa ctacgtcgac cacacgattc tgtcgaagat
      721 cgcgtgcaga cgagctcctt ttgctgcgtc gatcgaatcg acccacgcga tgcgcccgag
      781 caagtgcgcg cgaaagtctg gatgtccgtc gcggttctgc gcggccgggc cggtccgtgc
      841 gcagttgtgc aggatcgcgc gcagccgctc gacctcggcg cgcgggaccg acagcttgtc
      901 gttcacgacg aggcctgtca ggcgctgcgc cgcgctgcgc gtcatcacgc gcgtcttgcg
      961 gaaattcagc gcgaagccct cttcgaatgc gatcgcgccc acgcgggcga tgagcgttga
     1021 cgctgcgcgt gccagctcgc ggcggcccga gatcgcgatg tcatcggcgt agcgcgtgta
     1081 ccgcgcgccg atggttgccg cgaatgccgc gacgcgcacg tcgaaacgga acgcagcgag
     1141 gttcgcgagc gcaccggacg agggcgcgcc ctggggtaag tgcgctgtgc gcaggcgtgc
     1201 gtgcgtttca ccgtcgtgct gccggttggg cgtcgtatac aacacgtcgg gcggcgtgcg
     1261 atgcgtgcac agggctgcaa gcgttcgcgc gactcgctcg ggatacccgg cgctgtgaaa
     1321 gatcgcagcg acccgcgcac gcgtaacgga cgtgaagaac gcttgcaggt cgagtcgaat
     1381 gaccacatcg cgatcgacgt gaggcgtggc gaagctcgcg atcgaatggc cggcgcgaaa
     1441 cccatgggcg gcggtgtgcg gcggaatctg cgcgatgatg tcgtcgagca cgaaccgctg
     1501 gatcgtgcgc aggcgcggct ttggcgcttc gagcaagcga tagccgccgc gtttggcgat
     1561 ccaggtgtag cgataatggc ggcgccgttg atcgagagcc gtcggtgaca cgcggtggtg
     1621 gcgccgggca tcgctcgcgg tactcgagat gccacgcagg tcggcgaggc tcgtgagctg
     1681 gtcgacgctg agcccgagcc agacggcgag ctccttcgac gtcgcgagcg cggggacgcg
     1741 ccacggcgac tcggccatct cgggcggtgg agcgccacgc acgagcggtc gctcgcggac
     1801 tgcttcgtga agcgcgtagc tgtcgagcag cacctcgcgg agctcttcga gcgatgcagg
     1861 cccgtcgagc ggaaactcct cgacgatcag atcgacgagg tggtccatcc atggccggtt
     1921 cgcttcgaac acgtgctgca ggcgcgatag cagggcgtcg ccgttccatt cgccagcgag
     1981 cagcacatcc gcgagttgct ggacaaatcg ctccattacg agaagcgtgc ggcggcgcta
     2041 ccaaccagtc tcgtctggct cgaagggtcg tgcgtttcgc gcaccgccca ttcgtgcgtc
     2101 gcaatgtcga tctgaacaag catccccacg gtagccgcgg agaggtggcg aacatccgaa
     2161 gacgctcgaa cacccacttg gtagcgtcgc cgcacccgca gacgataccg caacgtcagg
     2221 cgacgaggac cgggcgcttg cgcagccact cgttcgacgt cagctgctgc aacatgaacg
     2281 cagcggtatc agcacgactg atcatcccgc cgctcagcga ggtgtcgtcg gcgacgcgcc
     2341 acttgccgcg cggtttgctg tcggtgagaa agccggggcg cacgacgatc cactcgagat
     2401 cggtctgcgc gagcgagcgc tccatcgcga gcttgtcgcg gaacgctttg cgcaacacga
     2461 gccagccacc gagccggatc acgaagccac cgcggtattc ggtcttctcg ccaacaccga
     2521 gcgacgacat cgcgacgatg cgcttcatgc cggcagattg catggcggcg atgatggtcg
     2581 cgatgccgcc cgagcagatg tcgacgtgac gccatggccg ggtgccgagg cacgacatca
     2641 cggcgtcgtg tcccttcacg gcagcggcga cggcggcgag gtcgaggaca tcgccgatga
     2701 tcacgcgcgg ctcgggtagt gaggagccgg cggacacttt ggtcgacccg gctgacgagc
     2761 cggtggacgc cgcggccgag ccgttggacg cggccgtcga gccgctgaac gagccggccg
     2821 agccggtgaa cgagccggcc gaggcgttga acgagccggc cgaggcgttg aacgcggcgg
     2881 acgagctggt cgacgcagcc gtcgagccgg tcgacgcagt ggtcaagccg atcgacgcag
     2941 ccggtgagcc gttcggcgcg gcgcccgagt gggccaacgc gggcgcggag gagggtgggg
     3001 ccgacgcccg gacacggcgt ggtgggagcg gacggcgtac gaacgcggtc atctcgtggc
     3061 cggccgctgc gccttggtcg agcaacgcgc ggccggtgtt gcccgtcgcg ccgaacacga
     3121 tcaccttcat cgcgcgagca ccatactgcg cgcgaacgcg gtgggcgagg cgagcggcac
     3181 gggagcgaac agtgcgctgt gcgaacgcgt gcctactcga aatcgagacg gtacttcagt
     3241 gacatgtctg catcactgaa gctcttccaa ttcgagacga gctggcagag gtctgcgttg
     3301 aagtcgtcac gtggaatgtg gacctcgaag tagccgtgcg cgtcggtcat cgtcgtgtag
     3361 cggccgacgg tgccggcaaa cgagatcgtg aagccttcgt tgagacgctc gaacgacgtg
     3421 aactgaccgc gaatcacgat gcccgagatc gtttcggagt cgcaggcgcg ctgggtcgac
     3481 tgtgtccgaa tctcgaccgg ctcgctcggg ggcgcaacgg caagctccgg cgcggacagg
     3541 gccggagcgg tcggttgtgt cagccaaatg gctgcggtag cgacggtagc gatccctagc
     3601 gacaggtact gcggcgcgcg aagggcgatc acctagtcat gatagcgcgc ggcctgcccg
     3661 aaatcctggg tcacttagcg ggggtaggag agctacacaa accgccgagc ttccgtaata
     3721 ttgaggtgcg atgaggatcc gtttgtcgga tgagaaaaag ccgcctggcc ccaagcggga
     3781 tggcgagcga cagacgggat tggtgctcga tgagcgcacg cgcaccaaga agccgccgat
     3841 gtacaaggtg ctgctgcaca acgacgacta cacgacccgt gagttcgtcg tgtgggtgct
     3901 gcagaccgtt ttccacaaga acgagagcga cgcgatcgcg atcatgagcc acgtgcacaa
     3961 ccacgggatc ggcgtcgcgg gcgtgtacac gttcgaggtc gccgagacca aggtcacgaa
     4021 aacgatgtcg ctcgcaaagg cccaacagtt tccgctgcaa ctctcgatag agccaaccga
     4081 ttgactagta ccggttggga tcgcggtccc ctacacttag gacgtcatgc tctcacctga
     4141 gctccaagcc acgctgcagc gcgccgtcga cgacgttcgt ggtcggcgtc acgagtacct
     4201 gacactcgag cacttgctgt tgtcgtgcct cgacgatccg accggcgccg acatcatcac
     4261 gaagtgcggt ggcgacgtcg agaagctccg cggcgacctc gagcggttct tggacgagca
     4321 agtcgaggag ctgccggacg gtgaggagtc gggaccggat cagacgctcg cgttccagcg
     4381 cgtgctgcag cgtgcggcga tgcacgtcca ggcggccggc cgccagcaga tgacgaccgg
     4441 caacgtgatc gcgtcgatgt tccgcgagcg cgactcgcat gcggtgtact tgctggagaa
     4501 gcagggcatc acccgcttcg atgtcatcaa ttacatctcg cacggcgtgt cgaaggtcga
     4561 tgccggtggc cccgtggtgc cgcggacccg tggcgtcgag gacgatggcc aggacgatca
     4621 aaagatctcg aacccgctcg agagcttctg cgtggacctc acgcagcgcg cacgcgaggg
     4681 caagatcgat ccgttgatcg gtcgcgccga cgagctcgag cgcatgatcc aggtcctgtc
     4741 gcggcgccgt aagaacaacc cgctgctgat cggtgagccg ggcgttggca agacggcgct
     4801 cgccgagggc ctcgcgctcc gcatcgtcga caagcaggtg cccgagccgc tgctcgacgc
     4861 gaaggtctat tcgctcgaca tgacggcggt gctcgcgggc actcgctatc gcggtgactt
     4921 cgaagagcgg ctcaaggccg tgatgaaggt gttgatcggc gatccgaagt cgatcctctt
     4981 catcgacgag atccataaca tcatcggcgc tggcgcgacg agcggcggga cgatggacgc
     5041 ggcgaacatc ttgaagccgc cgctgtcgaa cggcgagctc aagtgcatcg gctcgacgac
     5101 gttcaaggac tatcgccagg cgttcgagcg cgaccgcgct ctgtcgcgcc ggttccagcg
     5161 catcgacatc ggcgagccga cgatccccga ggcgatcgag atcctgaaag gtctcaagcc
     5221 gcgctacgag gagcaccaca acgtcaagtt caccgacgct gcgatcaccg cggctgtcga
     5281 gctgtctgcg cgccacatca gtggctcgca cttgcccgac aaggccatcg acgtgatcga
     5341 cgaggcaggc gcgaaggtgc ggctgatgcc cgccgaccag cgccccgacg agattcgccc
     5401 gaatctgatc gaggacgtcg tcgcgaagat cgcgcggatt ccgacgcgca gcgtgtcgac
     5461 gcaggacaag acgaagctcg cgaacctcga gggcgagctc aagcgcctca tcttcggcca
     5521 ggacgcggcg atcgagcagc tcgcgacggt gatcaagcta tcgcgcgcgg gtctcggcaa
     5581 tccgaacaag ccgaccggca acttcctgtt cgccggacct actggcgtcg gtaagacgga
     5641 gctcgcgaag cagctcgcgt cggtgctcgg catcgagttc atccgcttcg acatgtccga
     5701 gtacatggag aagcacacgg tgtcgcgctt gatcggcgcg cctcccggct acgtcggctt
     5761 cgatcaaggc ggccagctca ccgacgcgat caacaagaac ccgtactcgg tcgtcctgct
     5821 cgacgagatc gagaaggcgc accccgacat ctacaacatt ctcctgcaga tcatggatca
     5881 cgcgacgctg accgataaca acggtcgcaa gagcgacttc cgcaacgtga tcctgatcat
     5941 gtcgagcaac gcgggcagcc gcgagatggc ggcgggccgc attggctttg gcgattccat
     6001 cgcggacagc aaggacggca agaaggcact cgagcgcgtg ttcacgcccg agttccgcaa
     6061 ccgcctcgat gccaccgtgt tcttcggttc gctcccggtc gaggtgatcc gcaaggtcgc
     6121 ccgcaagttc ctcgacgagc tcgagggcca gctgcaggag aagaaggtct cgctcgaggt
     6181 cagcccggct gcgctcgact ggttcgtcga gcacggctac gacaaggcca tgggcgcccg
     6241 gccgatggcc cgcctcgtcc agagcacgct caaggcgcca ctcgccaacg agatcctgtt
     6301 cggtaaattg acgaatggcg gcgtggcaca cgtggatgtc aaagacggcc aaattgacat
     6361 cacgtgcgag ggtggcgacg ccccgaacta gcgcaagttg ctgcacagcg tcggttcggg
     6421 aattcgcttg ctaccggcac ggttgatgta gtgtcgtccg tcccgcatgg cgccattgat
     6481 cgagcttgtc gacatcgcgc gctactacca gatgggcgac gagcgcatcg cggcgctcga
     6541 tggcgtcagc ttcgcggtgc aggccggcga gatggtcgcg atcgtcggca cgtcgggatc
     6601 gggtaagtcg acgctgctca acgtcctcgg ctgcctcgat acgccgagtg ccggccggta
     6661 ccggctgaac ggccgcgatg ttcaggatct gtcagacgac gaccgcgcgc gggcacgcaa
     6721 ccaacagctc gggttcgtgt tccaaagctt tcaactgctg caccgcgcga ccgcactcaa
     6781 gaacgtcgaa ttgccgctgg tctatcgcgg catgccggcg aaggagcgcc gcgagcgcgc
     6841 gatgaaggcg ctgacccgcg tcggcctctc ggcgcgcatg aagcacaagc catatcagct
     6901 gtcgggtggc cagcggcagc gcgtcgcgat cgcacgcgcg ctcgtcagcg agccgtcgct
     6961 gctgctctgc gacgagccga ccggcaacct cgactcggcg acgaccgaag acatcatggg
     7021 gctgttccac cgcctgcacg acgagaagca cacgatcgtg atcgtgacgc acgatccgtc
     7081 gatcgcggcg cgctgtccgc gagcgatccg gatctccgac ggcaagatcc tcgccgatgg
     7141 tactggcgcc gacgtcgcgc gcggtgctgc gaccgtcagc gtcgccgtgc cggcagatac
     7201 ggtaggcgcg gcgtgacgaa gctcgatcgc ctcgcgctcg tagcgcttgt agcagcgtgc
     7261 agcggcggtg gtccgcggcg cgccggcaag gtcgaggagc gcgcgcgcgt cgagaaggtg
     7321 cgcatggccg aggttcgtga tcgcgtgctg ctcgccggca cggtgcacgc gatccagagc
     7381 gaagagctgc gcactccgcg caccccgatg tgggagctgc cggtgcgctg gatggcggag
     7441 gatggcgccg tcgtgaagaa gggcgatcgc gcgctggagt tcgacaactc gtcgttcgtc
     7501 agcacgctca aggagcagaa gctcgcgtac ctcgacgcgc tgatgacgtt cgacaccttc
     7561 ctcgacgtca gcgccctcga cgaagcgcag aagacgttcg agctcgacaa cgcgaagctc
     7621 acgctcgaaa aggcgcaggt gcaggcgaac gtgccagcag atctgttgcc ggcgcgcacc
     7681 gcgcaggagc gccagctcga gctgaagcgc gccgaggcgg ccgtcgagaa ggcacagaag
     7741 gagctcgcgt ccgcgaaggc agcgaatgcg ctcgagcggc aggtcaaaca gatcgagctc
     7801 gacaaggcaa agcagaagat cctcgacgcc gagaaggcga ttggcgagct cgatctgaag
     7861 gcgccgcgcg acggcatcat cgtcgttggc gaccacccgt ggatggggcg caagttccag
     7921 atcggtgaca ccgtgcagcc gggctggacc atcgtcgagc ttccggatct cacgtcgggc
     7981 atggaagtgc gcgccgagct gtcggacgtc gacgacggtc gcgtgatgat cggcatggcc
     8041 ggcacgtgca cgctcgatgc gtatccggcc gatggcgtgc cgtgcgtcgt gaaggacttg
     8101 atgccggtcg cgagcggcaa aggtggccag tcgctgcgtc gcgggttcgc gatcgtgctg
     8161 acgatcgaca aggtcgatca cgagaagatg cggccgggca tggcggtgaa ggtcgagctc
     8221 gcgcccgcga aggcgccagc gcagctcgtc gtgccgcgtg gtgcggtgca tttcgacaaa
     8281 gacaagacgc gcgtcaagct cgcgtcgggt gagctgcgcg acgtcgagct cggcgcgtgt
     8341 gacgcgcagg gctgcgtggt gacgaagggc ctcgcgagcg gcgacggtgt gtcgatcgga
     8401 ggcgagctgt gaagcgcgtg ctgctcgcgc tcgcggcgtt cgttgctctc ggcgcgtgca
     8461 accgcgagca cggcgatctc gcgctcgtcg acgtcaagaa ggcggatctc gtcgtcggcg
     8521 tcgaggtcaa cggcgagcta gcggccgtcg actcgctcga cgtcaagcca cccgcgctct
     8581 acgacacctg ggacttcaag atcgccgacc tcatcgccga gggcaaagag gtcaaagagg
     8641 gcgatccgat cgtcgcgttc gatccatcgg agcagatgcg cacgctcgag agtctggcga
     8701 acgacgccga cgccgcgaag aagaagctcg acaagaagcg cgacgatgcc gcgctcgccc
     8761 gccgcgacga gcagctgcag atcgcgcagg ccgaagcgac gctgcgcaag gcgtcgctga
     8821 agacgagcgc tgacaaggat ctcgtcgcga cggtcgatca gaaggtgctc gaaatcgacg
     8881 gcgcactcgc gaagctcgcg ctcgaccagg cgaagaacaa ggcggagcgc gccgcgaagt
     8941 cggacaccgc cgagatcaag agcctggccg accaccacga gtacctcgcg ggccgcgtcc
     9001 aggagttgca gcagaacatc gagaagatgg cggtcaaggc gccgcgcgcc ggcacgatcg
     9061 tctatccgac gaactggcgc ggcgagaaga agaaggtcgg cgacagctgc tggcgcatgg
     9121 aagcggtgct gcagatcgtc ggcctcggca agatggtcgg caacggcgaa atcgacgaga
     9181 tcgacgtcgc gcgcgtgaag gacaaccaac cggtgtcgat caagctcgat gcgttgcccg
     9241 acgtccagct gaagggcacc gtgcaggaga tcgcgaagtc ggtcgcgccg aaatcagagg
     9301 ctgatcgcag caacatcgcg aagctgaaga tcaaggtcga cgcaccggcg accgtgccgc
     9361 tcaggcctgg catgcgcttt cgcggcgagg tcgagaccga gcggctcgca aatgtcgtgc
     9421 aggtgcccgc cgacgccgta ttcgtcacgc ccgacgggcc ggtcgcgtac aagtcgacgg
     9481 gcgacggcgt gacgaaggtg aagctcgtgc tcggcaagcg caacgcgagc gcgatcgagg
     9541 tgaagagcgg gctcgcacct ggcgatcgcg tctcgcgcat cgatccggat cggagcgcgc
     9601 cgtgaagaag cttgtcgtcc tcgcggtgct tgccgccatc ggcggtggtg gctggttcgt
     9661 cgtccgccgc agcgtcggcg cggcgcagca tgatgacgtg ccgacgcaca cggtgaagaa
     9721 ggaagcgttc gtgcgccggg tgaccgcgga gggcaacctg cgcgcggtca aggcgacgcc
     9781 ggtcaccgca ccgcagagcg gcggcagctt cgggccgatg aagatcgcgt ggctcgcgcc
     9841 cgatggcgtc gcggtcaagg cgggcgatgt cgtcgtgcgc ttcgatccga cggatccgga
     9901 gaagcaattg aaggaagggc aggcggacct cgcagcggca tcggcaaagc tcgccgagga
     9961 agacatcaag tcgaagacgg cggtgcaggg acgcgacagc gcggccgacc tcgccggcgc
    10021 cgagctcgag cagcagcgca agttccagtc gaaggacaag gagatcttct cgcgcaacac
    10081 gatcgtcgag gccgaaatcg acgagaagct cgcgggtgcg aagcaagaac acgccgagca
    10141 gacgaagcag atcgagcgca agctcgcgca gtcgaaggcg gccgtgatcg cggtcgaaaa
    10201 gcagaaggcg cagatcgcga tctcgcacgc ggaagccgcg ctcaagagca tggaagtcaa
    10261 agcgccgcac gacggaatcc tcgtgttgcg ccgcaactgg cgtggcgagc tgccgcgcgt
    10321 cggcgagcag acctggcccg gccagaagat cgccgagatc ccgctgctcg acacgatgga
    10381 ggccgaggtg ttcgtgctcg aggtcgatgg caacggcatc accgagaagc agccggccga
    10441 gctcgtcgtc gaggcgaagc ccgagaatac gtacaaaggc acgatcaagc tcgtcgacaa
    10501 gctcgcgaag ccgcgcgtgc ccggtgtgcc cgtgcagtac ttcgcggtcg tgctcgagct
    10561 cgagaagacc gaccacgcgg tcatgaaacc cggccagcgc gtgcgcgccg agctcattct
    10621 cgacaaggcc gatgcgctcg tcgtgccgcg ccaggcagtg atcggcaaag agggcaagaa
    10681 cgtcgtctac aagcgcggcg cacacggctt cgagcccgtc gaggtcgagc tcggcgccgg
    10741 cacctcgggc cgcgtcgttg tcacgaaggg cctgtctgcg ggtgacgtga tcgcgctgcg
    10801 cgatccgacg cgctcgctcg atcaggcgat cggcagcggc agcgacggcg cgggcgaagc
    10861 ctccggcggc aaggggaagc aatgagacgc gtcctcgatg ccttcgtcgt cgcgctcgag
    10921 tcgctcgcag agcacaagct ccgcacgacg ctgacgatgc tcggcatgat gttcggtgtc
    10981 ggcgcggtga tcgcgatgct gtcgatcggc gcgggtgccg agaaagaagc gctcgcactg
    11041 atcgatcgcc tcggcacgcg caacgtcgtc gtgcgcagca agacgttcaa gccgaccgag
    11101 ctcgaagaga ttcgcaagaa gtcgctcggc ttgtcccagc gcgatgtcga agcgatcgag
    11161 gaagccgtgc cgggcgtgct gttcgcagca ccccgcatcg aggtcgagcc gtacaagatc
    11221 ctcgcggccg gtgccaagac gaaggcgcag gtctacggcg tctcgcaccg tcatcgcgaa
    11281 gtcacctcgt tcgcactcgc ggaaggccgc ttcctcgacg tcaacgacga gaagcaccac
    11341 ggacaagtct gcgtgatcgg cgcgggcgtg cgccgcgatc tgtttggcgc cgacgtcgcg
    11401 atcggccgcg acgtgaagat caacgacctg tggtgcgagg ttgtcggtgt gctcgcgccc
    11461 gagcccggcg cggtcaccgc ggtgcaaggc gtaccggtct cgtcgacgga gcgcgagatt
    11521 tacatgccgt tcacgacggc gctgcgcaag ctcgaccgcg atccgatgaa gtcgccgctc
    11581 ggcgagatcg tcgtgcgcct cgatcccaag gcccccgccg ccgagaccgg cgcggtcgtc
    11641 ggcacgctgc tcgatcgcct gcacggcggc gcgcgcgact acgacatcat cgtgcccgag
    11701 gcgctgctcc gccacagcca gcagacgcag cagttgttca acctcgtcat gggcctgatc
    11761 gcgggcatct cgctgctcgt cggcggcatc ggcatcatga acatcatgct cgcgtccgtg
    11821 ctcgagcaga cgcgcgagat cggcgtgcgg cgcgcagttg gcgcccgtcg ctccgacatt
    11881 cgcttccagt tcctcgtgac cgcgttctcg ctcgcgatgc tcggcggcct tgccggcgtg
    11941 gcgatgggcc tcgcgatcgc gcgcggtgtt gcgagctacg cgtcgtggcc gaccgtcgtg
    12001 accacgtggt cgatcgcact ctcgctcggc gtgtcgatcg tcgtcggcgt catctcgggc
    12061 ttgtacccgg caatgcgcgc ggcgcgcctc gatccgatcc aggcgctgca cgcggagtag
    12121 gctcagcggc gcgcacggcg tgcggcttcg agctggctgc gcgttagcac ggtatagagc
    12181 tgcttgtgac ctcgcgcttc gaacgtcgcc gcgatcgacc gagcctcttc ctccg
//
LOCUS       DASUFX010000417         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1596695, whole genome shotgun sequence.
ACCESSION   DASUFX010000417 DASUFX010000000
VERSION     DASUFX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596695"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFV99_16715"
     CDS             complement(<1..666)
                     /locus_tag="VFV99_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061013.1"
                     /translation="MANGSGAQLVGGEQIGPYRVLSHLAGNVYRAVHVDNGHRALLEL
                     APPDHWREAWLQMLHAQQLVDSLVHPGVARIVDRGMLPDRRAWVATEVPSGIGLGDLI
                     ARREMPPNELAMLIRDLAEVLAFAHAYGVVHRGLSLRSIVLATGKRSFPLVITDWGMR
                     TGGLGVFAAPELTTFAHYDGRADVYSLGVIAFRAATCMFPGEGGVFDVPGVPTGLATL
                     IARM"
     gene            complement(1011..2504)
                     /locus_tag="VFV99_16720"
     CDS             complement(1011..2504)
                     /locus_tag="VFV99_16720"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5061014.1"
                     /translation="MKTITHWIDGKLYERAAERSGDVFNPATGEVQAKVAFASPSVVD
                     EAVDSAWRASQTWRSISIAKRTKILFAFRQLVDKHQHELAKLLTLEHGKVTGDALGEV
                     NRGLEVIEFACGIADLAKGEYSENVSTEVDTYSIRQPLGVVAGITPFNFPAMVPMWMY
                     PIAIACGNTFVLKPSERDPSAAMFCAQLLADAGLPAGVFNVVHGDKVAVDRILEHPRI
                     AAVSFVGSTPIAQYIYETGTRHGKRVQALGGAKNHMIVLPDADMELAADAAVSAGYGS
                     AGERCMAISVVVAVGNAADKLVPLMKERIAKLKVGDGLEKGSEMGPLINKAHRDKVAG
                     YVDAGVKEGATLVSDGRALKVAGREAGFFLGPCLFDNVKPDMSIYKDEIFGPVLSTTR
                     VATYAEAIKLVNGNPYANGVAIFTNDGGAARKFQHEVEVGMVGINVPIPVPMAYYSFG
                     GWKASLFGDHHIYGRDGVHFYTRTKAVTSRWPDPSHRGVDLGFPQNK"
     gene            2670..3797
                     /gene="preA"
                     /locus_tag="VFV99_16725"
     CDS             2670..3797
                     /gene="preA"
                     /locus_tag="VFV99_16725"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX5061015.1"
                     /translation="MTDLSITVNGMKFENPFLLGSGPPGTNGKVIARSYDLGWGGMVC
                     KTFSLDASKVINTAPRYAKVKAKNHDEVIGFQNIELISDRPYEDWLVDLKQLKKQYPT
                     KILIASIMEEYRKEAWQQIVREVQETGVDGFEMNLSCPHGLPERKMGMAMGENPDIVE
                     EVVGWVKEVARIPVWAKMTPNVGNPTVPAASALKGGADGIAMINTILSVSGIDMKTLR
                     PTPTVEGHSVPGGYSGQCVRPIALRQVMEVARANPGVSISGIGGIETGYDAAQFFLLG
                     ASTVQVCTGAMLRGYEMISELKEELSKFMTDHKFANIREFVGKSLPYFTTHHDLVERQ
                     AAAREAKEAARAKSVGGKAQARDVETWKGDIAKETSTLVTG"
     gene            3810..4682
                     /locus_tag="VFV99_16730"
     CDS             3810..4682
                     /locus_tag="VFV99_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5061016.1"
                     /translation="MARKVLSGLIQASNPINDETKSVAEIQSAMFEKHLPMIHEAGKQ
                     GVQILCLQEIFNGPYFCPGQDKRWYDAAEAVPGPTVEKLRPIAAQYKMAMVVPLYERE
                     MAGVYYNTAAVIDADGTYLGKYRKNHIPQTSGFWEKYFFRPGNLGYPTFQTRYAKIGV
                     YICYDRHFPEGARALGLNGAEIVYNPSATVAGLSQYLWKLEQPAHAAANGYFVGAINR
                     VGTEAPWNIGKFYGTSYFVDPRGNFLATASEDKDELVVAEMNLDMIEEVRRTWQFYRD
                     RRPDTYDDMVKQLP"
BASE COUNT          880 a         1504 c         1541 g          784 t
ORIGIN      
        1 catgcgcgcg atcagcgtcg cgagcccggt cggcactcct ggcacgtcga atacgccacc
       61 ctcgccgggg aacatgcagg ttgcggcgcg gaacgcgatc actccgaggg agtacacgtc
      121 tgcgcggccg tcgtagtggg cgaacgttgt cagctcgggc gccgcgaaga cgccgaggcc
      181 gcccgtgcgc atgccccagt ccgtgatcac gagcgggaac gagcgcttcc ccgtcgcgag
      241 cacgatcgag cgcagcgaca gtccgcgatg gacaacgcca tacgcgtgcg cgaacgcgag
      301 gacctccgcg aggtcgcgga tcaacatcgc gagctcgttc ggcggcatct cccgccgcgc
      361 gatcagatcg ccgagcccga tgccactcgg cacctcggtc gcgacccacg cgcgtcgatc
      421 gggcagcatg ccgcgatcga cgatccgcgc gacgcccggg tgcacgagcg aatcgacgag
      481 ctgctgcgcg tgcagcatct ggagccaggc ttcgcgccag tgatcgggcg gcgcgagctc
      541 gagcagcgcg cgatggccgt tgtcgacgtg caccgcgcga tagacgttac cggcgaggtg
      601 gctgagcacg cggtacgggc cgatctgctc accgcctaca agctgcgctc cactgccgtt
      661 tgccatgcgt cggcgtggtt gtactgcgaa cggtttcgac tgcaagagat cgccctgtca
      721 gcttctttgc ttcgacccac cgattgcgag ctgttccgat cgggtttgtt ggccgcagcc
      781 aggcgaacgc agcttcagtt gaagatgcct cgcggaaaat agtgatgacg cccgctgccg
      841 agagaggctg gttgattcgc ggcagcggaa cgtcatcgat gatcgcgccg ccgcagtgca
      901 gcagccgtgc ctcgcgcaag cgcgcgaatc agcgcgcgcg agcaacatgg tcaaccgtcc
      961 ctggcgtcga ctgacccgga acgactgacc tcatatcgga ttcagttcca ctacttgttc
     1021 tgcgggaagc cgagatcgac gccgcggtgg ctcggatccg gccagcgcga ggtcaccgct
     1081 ttggtgcggg tgtagaagtg gacgccgtcg cgaccataga tgtggtggtc gccgaacagc
     1141 gacgccttcc agccgccgaa cgagtagtac gccatcggca ccggaatcgg cacgttgatg
     1201 ccgaccatgc cgacctcgac ctcgtgctgg aacttgcgcg cggcgccgcc gtcgttcgtg
     1261 aagatcgcga cgccgttcgc gtacggattt ccgttcacga gcttgatcgc ctcggcgtac
     1321 gtcgcgacgc gcgtcgtcga cagcacgggg ccgaagatct cgtccttgta gatcgacatg
     1381 tcgggcttca cgttgtcgaa caagcatggg ccgaggaaga acccggcctc gcggcccgcg
     1441 accttgagcg cgcgcccgtc ggagacgagc gtcgcgcctt ccttgacgcc ggcatcgacg
     1501 taacccgcga ccttgtcgcg atgcgccttg ttgatcagcg ggcccatctc cgagcccttc
     1561 tcgaggccgt caccgacctt gagcttcgcg atgcgctcct tcatgagcgg cacgagcttg
     1621 tcggcggcgt tgccgaccgc gacgaccacc gagatcgcca tgcagcgctc gcctgccgag
     1681 ccgtagccag cggagactgc ggcgtctgcg gcgagctcca tgtcggcgtc gggcagcacg
     1741 atcatgtggt tcttcgcgcc gccgagcgcc tgtacgcgct tgccgtggcg cgttccggtc
     1801 tcgtagatgt actgcgcgat cggcgtcgag ccgacaaacg agacagccgc gatccgcgga
     1861 tgctcgagga tgcgatcgac ggcgaccttg tcaccgtgca cgacgttgaa cacgccagcc
     1921 ggcaggcccg catcggcgag cagctgcgcg cagaacatcg cggccgatgg atcgcgctcg
     1981 ctcggcttga gcacgaacgt gtttccgcac gcgatcgcga tcgggtacat ccacatcggc
     2041 accatcgccg ggaagttgaa cggcgtgatg ccggcgacaa cgccgagcgg ctgacggatc
     2101 gagtacgtgt cgacttcggt cgagacgttc tccgagtact cgcccttcgc gaggtcagcg
     2161 atgccgcacg cgaactcgat cacttcgagg ccgcggttca cttcgccgag tgcgtcgccg
     2221 gtgactttgc cgtgctcgag cgtgagtagc ttcgcgagct cgtgctggtg cttgtcgacg
     2281 agctggcgga acgcgaacag gatcttcgtg cgcttcgcga tcgaaatcga gcgccatgtc
     2341 tgcgacgcgc gccaggcgga gtcgactgct tcgtcgacga cggaaggcga cgcgaacgcg
     2401 actttcgcct gtacctcgcc ggtcgccggg ttgaagacat cgccgctgcg ttccgcagcg
     2461 cgctcgtaca gcttgccgtc gatccagtgc gtgatcgtct tcatgaggac ggatcgcgta
     2521 ccacggggag taccgtcggt gaatctattt cgcgtcttgg ccgaaaagtg aaccgcgcgt
     2581 cacaaattcc gtcgagaaga agtaggttga acgctgctcg cggcctgtga tacccaaacc
     2641 gctttcccaa attgcaccct ggaggcgacg tgactgacct gtccatcacc gtcaatggca
     2701 tgaagttcga gaacccgttc ttgctcggct ctggaccgcc gggaacgaac ggcaaagtga
     2761 tcgcacgcag ctatgacctc ggatggggcg gcatggtctg caagacgttc tcgctcgatg
     2821 cgagcaaggt catcaacacg gcgccgcgct acgcgaaggt gaaggcgaag aatcacgacg
     2881 aagtgatcgg ctttcagaac atcgagctga tcagcgaccg tccgtacgag gactggctcg
     2941 tcgatctcaa gcagctcaag aagcagtacc cgaccaagat cctgatcgcg tcgatcatgg
     3001 aggagtaccg caaggaagca tggcagcaga tcgtgcgcga ggtccaagag accggcgtcg
     3061 acggtttcga gatgaacctg tcatgcccgc acggcttgcc ggagcgcaag atgggcatgg
     3121 caatgggcga gaaccccgac atcgtcgaag aggtcgtcgg ctgggtgaag gaagtcgcgc
     3181 gcattccggt gtgggcgaag atgacgccga acgtcggcaa cccgacggtt ccggccgcga
     3241 gcgcgctcaa gggcggcgct gacggcatcg cgatgatcaa cacgatcctc tcggtgtcgg
     3301 gcatcgacat gaagacgctg cgtccgacgc cgaccgtcga gggtcactcg gttccgggcg
     3361 gctactccgg tcagtgcgtg cgcccgatcg cgctgcgcca ggtgatggaa gtcgcgcgtg
     3421 cgaaccccgg cgtctcgatc agcggcatcg gtggcatcga gaccggctac gacgcggcgc
     3481 aattcttcct gctcggcgcg tcgacggtgc aagtgtgcac cggcgcgatg ctccgcggtt
     3541 acgagatgat cagcgagctc aaggaagagc tcagcaagtt catgaccgac cacaagttcg
     3601 caaacattcg cgagttcgtc ggcaagagct tgccgtactt cacgacgcac cacgacctcg
     3661 tcgagcgtca ggctgcggct cgcgaggcga aggaagctgc gcgcgcgaag tcggtcggcg
     3721 gcaaggcaca ggcacgtgat gtcgagacct ggaagggcga catcgcgaag gaaacctcga
     3781 cgctggtcac gggctaagga gacaagacaa tggcgcgcaa agttctcagt ggcctcatcc
     3841 aggcctccaa tccgatcaac gacgagacga agtcggtcgc cgagatccaa tcggcgatgt
     3901 tcgagaagca cttgccgatg attcacgagg ccggcaagca gggcgttcag atcctgtgtc
     3961 tgcaggaaat cttcaacggc ccgtacttct gccccggtca agacaagcgc tggtacgacg
     4021 cggccgaagc ggttcccggc ccgacggtcg agaagcttcg tccgatcgcg gcgcagtaca
     4081 agatggcgat ggtcgtcccg ctctacgagc gcgagatggc cggcgtctac tacaacactg
     4141 ccgcggtgat cgatgccgac ggcacgtacc tcggcaagta ccgcaagaac cacatcccgc
     4201 agacgagtgg cttctgggag aagtacttct tccgcccggg caacctcggc tatccgacgt
     4261 ttcagacccg ctacgcgaag atcggcgtgt acatctgcta cgaccgccac ttcccggagg
     4321 gcgcacgcgc gctcggtctc aacggcgcgg agatcgtcta caacccgtcg gcgacggtcg
     4381 ccgggctctc gcagtatctg tggaagctcg agcagcccgc gcacgcggcg gcgaacgggt
     4441 acttcgtcgg cgcgatcaac cgcgtcggca ccgaggcgcc gtggaacatc ggcaagttct
     4501 acgggacgag ctacttcgtc gatccgcgcg gcaacttcct cgcgaccgcg agcgaggaca
     4561 aggacgagct cgtcgtcgcc gagatgaacc tcgacatgat cgaggaggtg cggcgcacgt
     4621 ggcagttcta ccgcgaccgc cggccggaca cgtacgacga catggtgaag cagctgccgt
     4681 aggcccatga agctgcggcg gcgctccca
//
LOCUS       DASUFX010000418         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_900746,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000418 DASUFX010000000
VERSION     DASUFX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900746"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..>2266)
                     /locus_tag="VFV99_16735"
     CDS             complement(275..>2266)
                     /locus_tag="VFV99_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5061017.1"
                     /translation="LVMSSTSLLAVAPARADSPEETIVIIDSAPPRPSEDSSASASVI
                     TVDRTPRSAETMPDLLDDLPGVSVSRLGGIGAPALLSLRGSTWDQVSVYLDGVNLNLA
                     AGGGVDVSTLPVGDLARVEIYRGVTPIAYGGSAIGGVLAVETKRPQTDGATLEFGSGS
                     FGTWLGGGSVTFAREDYGIYVGLHSLKTAGNFDFVDDKGTAFNPDDDETIARHNNRLH
                     EHDAVIRGFAALPGPRELSLLVLGFDRDQGLPGYPRYATMQSSLQTRRAIGSIAYTSH
                     DELGTHSLLRAQLYGYGLEQRFRDPLSEIAVAPTDARDRTLALGGTVRASRAVSSWLQ
                     PAALLDIRRESFVPHDLIADERGAMSTRLLTVAGAEAALRKSGFTVTPSLRLEMSRDV
                     SAGRGDTGMLVAPDEPLTRTQPIVRVAITKPLRDDLMLRSNVGRYARLPSFLELYGNN
                     GFIVGNRELTPERGLTADVGGAYTGSQGPLALTADAAAFAVLIDDLIQFQQNAYGVAR
                     ARNIGHARVLGFESSAELRCEFARLYAQATLTDARDRSDSAAAADRQLPYRPRVHLTA
                     RPELRHALVGGTELGAYVEVDFTSGNFVDPANLVQLPSRMLLGAGASLGLDHGHVRFI
                     ASANNLTDASVSDVLAYPLPGRAFFLSMALTTDLSVKEN"
BASE COUNT          396 a          723 c          791 g          356 t
ORIGIN      
        1 cgacagcgcg agcgtataac ccggcgtgtg cgtgcccgag ttgatgcaat cgcccttctt
       61 gaccttggcc gtgctggggt cgagcaacga gatgctcgtc gacacgtagt cgctgttgac
      121 gacggcgagc cctgcgcctg gcgacatgac gccattgccg tcagccacgt gtgcatcgac
      181 atccatggac gctggttggg acttgtcgtt gcagccagcg agaccaacga ccgagcacag
      241 cgacactagc aagcagaaat gcagacgagt catatcagtt ctccttcacg gacaaatcgg
      301 tggtcagcgc catgctcaga aagaaggcgc gtcccggaag tggataggcg aggacatcac
      361 tcaccgacgc gtcggtgagg ttgttggcac tggcgatgaa acgaacgtgg ccgtgatcga
      421 gtccgagcga cgcacctgcg ccgagcagca tgcgactcgg tagctggacg aggttcgcgg
      481 ggtcgacgaa gttgccgctc gtgaaatcga cttcgacgta cgcgccgagc tcggtgccgc
      541 cgacgagcgc atggcgcagc tcggggcgcg cggtgagatg cacgcgcggt cgatagggca
      601 gctggcgatc ggcagccgcg gcgctgtcgc tgcgatcgcg cgcatcggtc aacgtcgcct
      661 gcgcgtacag gcgcgcgaat tcgcaacgca gctcggcgct ggactcgaag ccgagcacgc
      721 gcgcatggcc gatgttgcgc gcacgcgcaa caccgtacgc gttctgctgg aactggatca
      781 gatcgtcgat gagaactgcg aacgccgctg catcggcggt cagcgcgagc ggtccttgcg
      841 agccggtgta tgcgccaccg acgtcggcgg tgagcccacg ctcgggcgta agctcgcggt
      901 tgccgacgat gaagccgttg ttgccataca gctcgaggaa gctcggaagt cgcgcatagc
      961 gacctacgtt cgagcgcagc atcagatcgt cgcggagcgg cttcgtgatc gcgacgcgca
     1021 cgatcggctg cgtgcgggtc agcggctcgt cgggcgcgac gagcatgccg gtgtcgccgc
     1081 ggccggccga gacgtcgcgc gacatctcga ggcgcaacga tggcgtcacg gtgaagccag
     1141 atttgcgcag cgccgcctcg gcgcccgcaa cggtcaacag ccgcgtcgac atcgcgccgc
     1201 gttcatccgc gatcagatcg tgcggcacga acgattcgcg gcggatgtcg aggagtgcag
     1261 ccggttgtag ccacgatgac accgcgcgac tcgcgcgcac cgttccgccg agtgcgagcg
     1321 tgcggtcgcg cgcatcggtc ggcgccactg cgatctccga cagcggatcg cggaatcgct
     1381 gctcgaggcc gtagccgtag agctgcgcgc gcagcaggct gtgggtgccg agctcgtcgt
     1441 gcgacgtata cgcgatgctg ccgatcgcgc gccgcgtctg cagcgatgat tgcatcgtcg
     1501 cgtagcgcgg atacccggga agtccttggt cgcgatcgaa gccgagcacg agcagcgaca
     1561 gctcgcgcgg accgggcagg gcggcaaagc cgcggatgac cgcgtcgtgc tcgtggagcc
     1621 ggttgttgtg acgagcgatc gtctcgtcgt catcggggtt gaatgcggtg cccttgtcgt
     1681 cgacgaagtc gaagttgccc gcggtcttca gtgaatggag cccgacgtag atgccgtagt
     1741 cctcgcgggc gaacgtcacg ctgccaccgc cgagccacgt gccgaacgag ccgctgccga
     1801 attcgagcgt cgcgccgtcg gtctgcggtc gcttcgtctc gacggcgagc acgccgccga
     1861 tcgcggagcc gccgtatgcg atcggcgtga cgccgcggta gatctcgacg cgtgcaaggt
     1921 cgccgaccgg caacgtcgag acgtcgacgc cgccgcctgc agcgaggttc aggttgacgc
     1981 cgtcgagata gacgctgacc tgatcccacg tcgatccgcg caacgacagc agcgccggcg
     2041 cgccgatgcc acccagacgc gacaccgaga cgccgggcag atcgtccagc aggtcgggca
     2101 tcgtctcggc gctgcgcggc gtgcgatcga cggtgatcac cgatgccgac gccgaactgt
     2161 cctcggatgg acgcggcggc gcgctatcaa tgatgacgat cgtctcttcc ggtgagtcgg
     2221 cacgagccgg cgccacagca agcagcgacg tcgagctcat cacgag
//
LOCUS       DASUFX010000419         5485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1040283, whole genome shotgun sequence.
ACCESSION   DASUFX010000419 DASUFX010000000
VERSION     DASUFX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040283"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..845)
                     /locus_tag="VFV99_16740"
     CDS             complement(237..845)
                     /locus_tag="VFV99_16740"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5061018.1"
                     /translation="MVPDPDSSDFDAVAAAKELEELLGESTSSGGAKDDSYTAGLEAD
                     IEELNALIAKKDAELARANARAEQAHAEIEAAGNRLAIASAKELEQRTRKLLETFLPV
                     IDDLDRAIAAARTHVESADVVAGLDLVRRSMLTRLGQFGVTHVPALGEKFDPQRHDAI
                     AMVPVTDSAQDGRVIDVVREGYLIGEDTLRPAGVAVGKLAAR"
     gene            916..1476
                     /locus_tag="VFV99_16745"
     CDS             916..1476
                     /locus_tag="VFV99_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061019.1"
                     /translation="MNRPLFARALVALCFVGTACGKSGAGDVRSKWLAAKLVPTAAET
                     KGVKLHVDIPEGLPENKESIVGPDWMVKPVGEAGPRIGISLREKTFKTPEDLAREVEP
                     DAKRADLVEVSKLDQGGGRLQYVSAVKNNRHLDVTVWIPTDETHGLEASCHWYSGPDD
                     KKSTDKPDAELIAWLTKICDSIKLGS"
     gene            complement(1481..1912)
                     /locus_tag="VFV99_16750"
     CDS             complement(1481..1912)
                     /locus_tag="VFV99_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061020.1"
                     /translation="MAMTLDDVDAFATSLPGVTVGAKWGNRTWLVEDKGFAWQRPFSK
                     ADIKRYGDETPPQGEILAVRVESLDAKDALLAIAPPGFFTIPHFNGYAAVLIALRQAR
                     TKDVRAAIRDAFSTVAASLPAAKPTHARKRATPRRTRAKKR"
     gene            complement(1971..2810)
                     /locus_tag="VFV99_16755"
     CDS             complement(1971..2810)
                     /locus_tag="VFV99_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061021.1"
                     /translation="MSEQTDAPTETTTAADAPPQAEANTPPQVEANVSPQEPAEASAP
                     APAPKPGYPFSVLKVLGTALRVTKRNFVPFLVLACVLDLPVILLRLSGQIEDVVVALV
                     LQLITHALMTAVVAYGVIMELHGSRPSTRACIAKGFAQIGPVVGVTVISTLAIGAATL
                     LFVIPGIIVGLMFFVIVPVTVVEGLGIDAAMKRSSELTRGRKGDLFLIMTFAGALGVA
                     IELVAQFELGPEAAFVWRAVGGAFSSMFLAVAVGVVYVELRKLRDGIQIPDIATAFAR
                     IRK"
     gene            3176..4810
                     /locus_tag="VFV99_16760"
     CDS             3176..4810
                     /locus_tag="VFV99_16760"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061022.1"
                     /translation="MKLKPLFAVVGVFAGCADHPYDPDAPAFDRNAPRVHIVSPARGT
                     IAGDVTHVLVGGTASDDLGIKAVSVNGAQAAVADDGTWVVDVGVAPGTTLLHAVAVDE
                     QGNQGEETRAVVAGPMLSVARHIDSGIRAAVSAPALNALGRGTATFIENGGLMTAVLG
                     MNPVVDVGGGPDCLYAQASITALTMHDADVLMGPTNGAVLVSAVLNDVRVGVHLQWAV
                     SCVDGARDVMLTADRVTVNGRLAVGVINHSLDVQFPNPQVQVTGFNLVSEDVPDGIVQ
                     MLGLDAAIGPILGSMTGRLVTPMVERSLSALDDAKTLDVAGTKIDVDVQPTQVNFTDL
                     GGTIILDSSFRAQGDSGGFVLVPNNPPALGMTHGFEIAVADDAANQLLTSMWSAKAFD
                     GTLDLATSPYEDISKLYDIVQLQMKLPPYVDASHRPLELTIGDWIATFSGDGVVPTTV
                     AIHAKSALYVVEGDDGKLRIDLSTPLVTIDVEGDGQFITKGQYDAIKSFAVEHIKTIG
                     SAAVATIPLPVIGDVTPTNLWVDPESDYLLIVGEIQ"
     gene            4820..5449
                     /locus_tag="VFV99_16765"
     CDS             4820..5449
                     /locus_tag="VFV99_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061023.1"
                     /translation="MRYFLAIALFATGCMELPLGGPRYDSVRERMSEAPTSLFIHDEM
                     SAGSITAQRRGSDGWIAGTTDVTVDHGYVRAALDGNGQLEIDQLEIALAPISLDGVFA
                     KPAELQNVILRLTHSTRADATWTSSDNASATLPMTFDFDWWIAIDGEAPIPLATQQLP
                     SINVGVDLGGNGDVVEASVDISASGVLWNWADLVQITSLDLTLGAKTAD"
BASE COUNT          994 a         1785 c         1730 g          976 t
ORIGIN      
        1 agcgcgtgga ccatcgggtg ataacgctcg atcaacagct cgagtgcctg tcgctcaccc
       61 tgctgcgcgc gtcgcagcgt gaggtcgtcg agctcggcgg ccgagctgga gcgaactgcg
      121 gtctgtgcgc gtcccatcat ctagttgcac acgcgagcgt tcgatcggtt ccacgaaatc
      181 gaccgcgctg ccaccagcac agtaacccag cgagtctgct gccgatcggg cgcaggtcag
      241 cgcgcggcga gcttgcctac agcgacgcca gctgggcgca acgtgtcctc gccgatgaga
      301 tagccctcgc gcacgacgtc gatgacgcga ccgtcttgcg cggaatcggt gacgggaacc
      361 atcgcgatcg catcatgccg ctgcggatcg aacttctcgc cgagcgctgg cacgtgtgtc
      421 accccgaact ggccgaggcg cgtgagcatg ctgcgccgca cgagatcgag ccccgcgacg
      481 acgtccgccg attccacgtg cgtcctcgcg gctgcgatcg cgcgatcgag atcgtcgatg
      541 acgggcaaga acgtctcgag tagcttgcgc gtgcgctgct cgagctcctt cgccgacgca
      601 attgccagcc gatttccagc agcttcgatc tcggcgtgcg cttgctcggc acgtgcgttc
      661 gcgcgcgcga gctcggcgtc cttcttcgcg atcagcgcgt tcagctcctc gatgtcggct
      721 tcgaggccag ctgtgtagct gtcatccttg gcgccaccac tcgacgtcga ctcgccgagt
      781 agctcctcta gctccttggc tgctgcaacg gcatcgaagt cactcgaatc aggatcgggc
      841 accatgcacg cagcgtagcc gacgattggc ggtgcgcctg ttgagaaagc cgcacgaacg
      901 cactatatcg gggccatgaa tcgaccgctt ttcgctcggg cgctcgtcgc gctctgcttc
      961 gtgggtacag cgtgtggcaa atcgggcgcc ggcgacgtgc gcagcaagtg gcttgcagcg
     1021 aagctcgtgc cgacggccgc cgagacgaag ggcgtgaagc ttcacgtcga cattccggag
     1081 ggcctcccgg aaaacaagga atctatcgtc ggtcccgact ggatggtgaa gccggtcgga
     1141 gaggcgggac cgcgaatcgg catctcgctc cgcgaaaaga cgttcaagac tcccgaggac
     1201 ctcgcgcgcg aagtcgagcc cgatgccaag cgtgccgatc tcgtcgaggt cagcaaacta
     1261 gaccagggcg gcggccggct gcagtacgtg tcggccgtga agaacaaccg ccacctcgac
     1321 gtcacggtgt ggattccgac cgatgaaaca catgggctcg aggcgtcgtg tcactggtat
     1381 tcgggaccag acgacaagaa gtcgaccgac aagccggacg cagagctgat cgcctggctc
     1441 acgaagatct gcgacagcat caagctcggt agctgagtcg ctaacgcttc ttcgcgcgcg
     1501 tgcgccgagg cgttgcgcgc ttgcgcgcat gggtcggctt cgcggccggc agcgatgcag
     1561 cgaccgtgct gaacgcgtcg cggatcgcgg cacgcacgtc cttggtacgc gcctgtcgca
     1621 atgcgatcaa cacggcggcg tagccgttga agtgaggaat cgtgaagaag cccggcggtg
     1681 cgatcgcgag caacgcgtcc ttggcgtcga ggctctcgac gcgcaccgcg aggatctcac
     1741 cttgcggtgg tgtctcgtcg ccgtagcgct tgatgtcggc cttgctgaat ggtcgctgcc
     1801 acgcgaagcc cttgtcctcc accaaccatg tgcgattgcc ccacttcgca ccgacggtga
     1861 caccaggcag gctcgtcgca aacgcgtcga catcgtccaa agtcattgcc atggttcggc
     1921 gcgagcttag cgtacgtcgc ggacttcgtg acggagttgc agctcacctg tcacttgcga
     1981 atccgcgcga acgcggtcgc gatgtcggga atctgtatgc catcacgcag cttgcgaagc
     2041 tcgacgtaga cgacgcccac ggcgaccgca aggaacatcg acgagaatgc cccaccgacc
     2101 gcacgccaca cgaatgcagc ctcgggtccg agctcgaact gtgcgacgag ctcgatggca
     2161 acgccgagtg cgcccgcaaa cgtcatgatc agaaacaggt cacccttgcg cccacgcgtg
     2221 agctcgctgc ttcgcttcat cgcggcgtcg ataccgagcc cctcgacgac ggtaaccggc
     2281 acgatcacga agaacatgag cccgacgatg atcccgggaa tgacgaacag gagcgtcgcg
     2341 gcaccgatcg cgagtgtcga gatcaccgtc acgccgacca cggggccgat ctgagcgaac
     2401 ccttttgcga tgcatgcacg tgtcgatgga cgcgagccgt ggagctccat gataacgccg
     2461 tacgctacga cagccgtcat cagcgcatgc gtgatcagct gcagcacgag cgcgacgaca
     2521 acgtcttcga tttgcccgct gagcctgagg agaataaccg gtaggtcgag aacgcaggcg
     2581 aggaccagga atggcacgaa gttgcgcttg gtgactcgca gcgccgttcc aagcaccttc
     2641 aggacgctga acggatagcc gggcttgggt gccggtgctg gcgcgctcgc ctcggccggc
     2701 tcttgtggcg acacgttcgc ttccacttgc ggcggcgtgt tcgcctctgc ttgcggcggt
     2761 gcatcggcgg cagtcgtggt ctctgtaggc gcatctgtct gctcgctcat cacgtcgatg
     2821 atacgccagc gagcgcaacc aaatgttgcg ggtgttcgcg tcgattcatt tcgcatcgat
     2881 gtaggacaca cggatgcgag cgcacgcgca cgatcgagag atccgcgtca caacgacgag
     2941 cgatcaaacg aagtccacac cgatgctggg ctcgctgttc aggctggcta gtcgactggt
     3001 cgggtagccg cgcatgcagc gaagttacgc cgccgaccga gaccaagtgt cacgtatctt
     3061 actcactact gactttcgtt ctataacgac tactccaatc atttcggcgt atcggcaggt
     3121 tttgtccaca tgatcttcgc agatggtcag ttcattgcaa cgcactgcgt tagccatgaa
     3181 actcaaacca ctgttcgcag tcgtgggtgt ctttgccggc tgtgccgacc acccctacga
     3241 tccagacgcg ccggcgttcg atcgcaacgc accacgcgtg cacatcgtat cgcccgcgcg
     3301 cggcacgatc gctggcgacg tcacgcacgt gctcgttggc ggtaccgcga gcgacgatct
     3361 cggcatcaag gccgtctccg tgaacggcgc gcaggcagct gtcgccgacg acggcacgtg
     3421 ggttgtcgat gtcggcgttg cgccgggcac gacgctcttg cacgccgtcg cggtcgacga
     3481 gcaaggcaat cagggcgagg agacgcgcgc ggtcgttgcc gggccgatgc tctcggtcgc
     3541 tcgtcacatc gacagcggta tccgcgctgc agtgtcagca cccgcgttga atgcactcgg
     3601 ccgcggcacc gcaacgttca tcgagaacgg cggcctcatg accgctgtcc tcggcatgaa
     3661 tcccgtcgtc gatgtcggcg gtggtcccga ctgcctctat gcgcaggcgt cgatcacggc
     3721 actgacgatg cacgacgccg acgtgctgat gggcccgacg aacggcgctg tcctcgtgtc
     3781 ggctgtgctc aacgacgttc gcgtcggtgt tcacctgcag tgggcggtct cgtgtgtcga
     3841 cggcgctcgc gatgtcatgc tcacggccga tcgcgtgacc gtgaatggcc ggctcgccgt
     3901 cggtgtcatc aaccactcgc tcgacgtgca gttcccgaat ccgcaggtgc aagtcaccgg
     3961 gttcaatctg gtgtccgaag atgtgcccga cggcatcgtg cagatgctcg gtctcgacgc
     4021 tgcgatcggt ccgattctcg gctcgatgac ggggcgactc gtcacgccga tggtggagcg
     4081 ctcgctctcc gcgctcgacg acgcgaagac gctcgatgtg gccggcacca agatcgacgt
     4141 cgacgtccag ccgacgcagg tcaacttcac cgacctcggc ggcacgatca ttctcgacag
     4201 ctcgttccgc gcgcagggag acagcggtgg gttcgtgctc gtgccgaata acccgccggc
     4261 gctcggtatg acgcacggct tcgagatcgc tgtcgccgac gatgcagcga atcagctgct
     4321 cacgagcatg tggtcggcca aggcattcga cggcacgctc gatctcgcga cgagcccgta
     4381 cgaggacatc agcaagctct acgacatcgt gcagctgcag atgaagctgc ccccgtacgt
     4441 cgacgcgagc caccgtccac tcgagctgac gatcggcgac tggatcgcca cgttcagcgg
     4501 agacggcgtc gtgccgacga cggttgcgat tcacgcgaag tcagcgctct acgttgtcga
     4561 aggcgacgac ggcaagctgc gcatcgacct cagcacgccg ctcgtcacga tcgacgtcga
     4621 gggcgacggt cagttcatca cgaagggcca gtacgacgcg atcaaatcat tcgcggtcga
     4681 gcacatcaag acgatcggct cggctgcagt cgccacgatc ccgctgcccg tgatcggcga
     4741 cgtaaccccc accaacctgt gggtcgaccc cgagtccgac tatctgctga tcgtcggcga
     4801 gattcagtag gaggctgcca tgcgttactt cctggcaatc gcactgttcg caactggctg
     4861 catggaacta ccgctcggcg gaccgcgtta cgactcggtt cgcgagcgga tgtccgaagc
     4921 gccgacctcg ctcttcatcc acgacgagat gagcgccggc tcgatcaccg cgcagcgacg
     4981 tggctccgac ggctggatcg ccggcaccac cgacgtgacg gtcgatcacg gctacgtgcg
     5041 cgccgcactc gacggcaacg gccagctcga gatcgatcaa ctcgagatcg cgctggcgcc
     5101 gatctcgctc gacggcgtgt tcgccaagcc cgcggagctg cagaacgtga tcttgcggct
     5161 cactcactcc acccgtgcgg acgcgacgtg gacgtcttcg gacaacgcgt ccgcgacgct
     5221 gccaatgacg ttcgatttcg actggtggat cgcgatcgac ggcgaggcac cgattccgct
     5281 ggcgacgcag cagctgccgt cgatcaacgt cggcgtcgat ctcggcggta atggcgacgt
     5341 cgtcgaggcg tcggtcgata tttcggcatc cggcgtgctg tggaactggg ccgacttggt
     5401 ccagatcacc tcgctcgatc tcacgctcgg cgcgaagacg gccgactaac cgccgctcgc
     5461 gcaccagcgc gcagttcgag caccg
//
LOCUS       DASUFX010000420         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1317239, whole genome shotgun sequence.
ACCESSION   DASUFX010000420 DASUFX010000000
VERSION     DASUFX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317239"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..714
                     /locus_tag="VFV99_16770"
     CDS             154..714
                     /locus_tag="VFV99_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5061024.1"
                     /translation="MGPSAAIRFACAALLLVGCADYLDLDPPLLAPDAAVDTRNDAST
                     VTPPDAAGCPAAPAGCTLFQCSATSSCYYACSGTGSWSTAQSYCTQVGDLVTIQSQVE
                     QDCVVAAAKPTSGSPVWIGAHQTPDASEPAQGWTWAAGTSFYTHWGSYQPDDFYGTED
                     CAELSGGGMWNDAACSYDRRFVCEVP"
     gene            796..2403
                     /locus_tag="VFV99_16775"
     CDS             796..2403
                     /locus_tag="VFV99_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061025.1"
                     /translation="MRGKDLSGEVLEDRYRVLERIAEGAMGAVYRGTRLNIDRAVAIK
                     VMHASLPSAMEGRKRFEREAKLMARLEHPHCVSLIDYGLHGAKPYVVMELVRGRSLHD
                     ALVEQGKFEITRAIDVMRQVLSGLAHAHEQGIIHRDIKPANIMVTPKAPLGLHVRILD
                     FGLARMLEASTSVSNGVAVGTPSYMAPEQCRGEELDARVDIYACGVVLFEMLTGKKPF
                     IAGDPIAIVKKQIDQPPPHLRDIPPGGDYGALEDIVARALSKSAADRFPSAVAMAEAL
                     EAAVSGRPATAEPTMVLPVTGDPSSSASKEKTVTLAPPSASMDVPITVGSSVIQKPPK
                     PGSSVRRQLPVSRTRWFVLAALLALGAGVAFVVVNYDDWLGAKKPTEADAAFTRVVDV
                     PKPPDAAPPSDPATDLAKQASDLVAAGKQQAAIDSLVKARKVYPDSAILALTLGKLYM
                     GKLWWADGIANLRDAIKLDPNVKEDDEVIKLTVRGFLMTPSYDYRLANLLIEIGPPAK
                     PALAEAAKSHPNPPTRARAESLLRRMH"
     gene            complement(2414..3805)
                     /locus_tag="VFV99_16780"
     CDS             complement(2414..3805)
                     /locus_tag="VFV99_16780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5061026.1"
                     /translation="MRRIADVPTVLRYLSIALLLLAIILFWRMCREPARTSQAIRLAP
                     IHIKYRPGMVRYQTMAHAYTVRPLFRHAHQSVAAYDDPRDRWVRIEVDTRYTKQILAQ
                     LANDPAVETAFVPPTVTLPRRPVVQADDESCPIHTPSFESYQGYLGPAPHGIDAPAAW
                     RRGVRGQGIWFADIEGGWNSKHEDLPGDRISHVAGEPINDIHWRAHGTAVLGEVVGRD
                     NGKGVVGIAPDVERVFTSSIGGIPTAEALDAAAEQLRAGDVLLIELQGAGPRGRYLPM
                     EYWDDNFDAIEAATSRGVLVIEAAGNGGENLDHKIYKGKLSRSGRDSGAIMVGAGGPP
                     RDGFDDRERLDFSNYGTRVDVQGWGRMVATLDYGDLQNCAGTERSYRDRHYTNEFSGT
                     SSASPIVAGAAVLIESYAKQQGRVLSPTEVRDILSHTGTPQAGDTTQNIGPRPDLARA
                     LLRVQRYADQLAL"
     gene            complement(3843..4382)
                     /locus_tag="VFV99_16785"
     CDS             complement(3843..4382)
                     /locus_tag="VFV99_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5061027.1"
                     /translation="MYSPERYVDAMRFAAAAHNAQKEPGHELPYLVHVVSVAAEVIAA
                     LPAMNGIDADLAVTCALLHDTVEDTKVTLDEIRARYGDAVAAGVSALTKNQKLETKPE
                     QMADSLRRIKEQPREIAIVKLADRITNLSEPPHYWTKEKCAAYRLEAGLIADTLGYAC
                     PVLEARLRARIAAYVQYCQ"
     gene            complement(4398..>5005)
                     /locus_tag="VFV99_16790"
     CDS             complement(4398..>5005)
                     /locus_tag="VFV99_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061028.1"
                     /translation="VAPAPAVAIVDERKPVATRQHVVVQETPLQEARTEEPQPATTAT
                     AAPERGGAAAPVQQVSPQHNVAPAKQNVAPATGPNEVERRFQAGMALLKSGKSAEAAR
                     ELLAAADAGGDDPLAADARYFAAAALTKAGRKTEAERALVAFIDRAPTSIRRGRAAVM
                     LARLIAERGDNAAARAWFESALGDADASVVAAARAGIAALH"
BASE COUNT          846 a         1657 c         1637 g          865 t
ORIGIN      
        1 ctgcggagaa caggttgcga cacgctgtat acatcacagt cgttctggcc atccctaacc
       61 gattaacttt actgatttca tgacgtaaca tgaccgaggc gcgctcattc tggtagacgt
      121 tctgtttacc gttgtgatag atcggaacac agagtgggtc cttcggctgc catccgtttc
      181 gcgtgtgccg cgctgctgct cgtcggctgc gccgattacc tcgatctcga tccgccgcta
      241 ctcgctcctg acgcagccgt cgacacccgc aacgacgcca gcacggtgac gccacctgat
      301 gccgcggggt gccccgctgc accggcgggc tgcacgctgt tccagtgctc ggcgacgtcg
      361 agctgttact acgcatgcag cggcaccggc agctggagca cggcccagag ctactgcacg
      421 caggtcggcg acctcgtcac gatccaatcg caagtcgaac aagattgcgt cgtggcggca
      481 gcgaagccga cgagcggaag cccggtctgg atcggtgcgc atcaaacgcc ggatgcttcg
      541 gagcccgcgc aaggctggac gtgggccgcg gggaccagtt tctacacgca ctggggcagc
      601 taccagcccg acgatttcta cgggaccgaa gactgtgcag agctgagcgg tggcggaatg
      661 tggaacgatg ctgcgtgcag ttacgaccgg cggtttgtgt gcgaggtgcc ttaactttcg
      721 atcacagata actacgcaga tctgcgtgcc caccgactag cctgcctaca tcgaaccgct
      781 gttattctgg ccgccgtgcg tggaaaggat ctcagtggcg aggtcctcga agaccgctac
      841 cgcgtcctcg agcggattgc ggaaggggcc atgggagcgg tgtatcgcgg cacgcgcctg
      901 aatatcgatc gcgccgtcgc gatcaaggta atgcacgctt cgctcccgtc ggcgatggaa
      961 ggccgcaaac ggttcgagcg cgaagcgaag ctgatggcac ggctcgaaca cccgcactgc
     1021 gtctcgctca tcgactacgg cctgcacgga gcgaagccct acgtcgtcat ggagctcgtt
     1081 cgcggccgca gcttgcacga cgcgctcgtc gagcagggca aattcgagat cacgcgcgcg
     1141 atcgatgtca tgcgccaggt cctatccggg ctcgcccacg cacacgagca aggcatcatc
     1201 catcgcgaca tcaagccggc gaacatcatg gtcacgccga aggcgccgct cggcttgcac
     1261 gtccgcattc tcgacttcgg gctcgcgcgc atgctggagg caagtacgag cgtgagcaac
     1321 ggcgtcgcgg tcggtacgcc gagctacatg gcacccgagc aatgccgcgg cgaagagctc
     1381 gacgcgcgcg tcgatatcta cgcgtgcggc gtcgtgttgt tcgagatgct gaccggcaag
     1441 aagccgttca tcgcgggcga tccgatcgcg atcgtgaaga agcagatcga ccagccgccg
     1501 ccgcatctgc gcgacatccc acctggtggc gattacggcg cactcgagga catcgttgcg
     1561 cgcgctctct cgaagtctgc cgccgatcgc ttcccatccg cggtcgcgat ggccgaggcg
     1621 ctcgaagccg ccgttagcgg ccggcccgcc accgcggagc cgacgatggt gctgcccgtc
     1681 accggcgatc cgtcgagctc ggcttcgaaa gagaaaacgg tgacgctcgc gccgccatcg
     1741 gcctccatgg acgtgccgat cacggtcggc tcgtcggtga tccagaagcc gccgaagccc
     1801 ggtagctccg tgcgccgcca gctgccggtc tcgcgcacgc gctggttcgt gcttgccgcc
     1861 ctcctcgcac tcggcgcggg cgtcgcgttc gtcgtcgtca actacgacga ctggcttggt
     1921 gccaagaagc cgacggaagc cgatgctgcg ttcacgcgcg tcgtcgacgt gccgaagcca
     1981 cccgacgcgg cgccgccgag cgatccggcg accgacctcg cgaagcaagc aagcgacctc
     2041 gtcgctgccg gcaaacagca agcggcgatc gactcgctgg tgaaagcgcg caaggtctac
     2101 cccgatagtg cgatcctcgc gctcacgctc ggcaagctct acatgggcaa gctgtggtgg
     2161 gccgatggca tcgcgaacct gcgcgatgcg atcaagctcg acccgaacgt gaaggaagac
     2221 gacgaggtca tcaagctgac ggtccgcggc ttcctgatga caccgagcta cgactatcgg
     2281 ctcgcgaatc tcttgatcga gatcggcccg cccgcaaagc cagcgctcgc agaagccgcg
     2341 aagtcgcatc cgaacccgcc gactcgcgcg cgcgcagaat cactgctgcg ccgcatgcac
     2401 tgagtcgcgc gcgctacagc gccagctgat ctgcgtaacg ctggacgcgg agcaacgcgc
     2461 gagcgagatc cgggcgcggg ccgatgttct gcgtcgtgtc gccggcttgc ggcgtgccgg
     2521 tgtggctgag gatgtcgcgc acctccgtcg gcgacagcac gcgaccttgt tgcttcgcgt
     2581 agctctcgat gaggacggcc gcgcccgcga cgatcggcga cgcgctcgac gtgccggaaa
     2641 actcgttcgt gtagtgacga tcgcgatagc tgcgctcggt cccggcgcag ttctggaggt
     2701 cgccgtaatc gagcgtcgcg accatgcgtc cccagccctg cacgtcgacg cgcgtgccgt
     2761 agttcgagaa atcgaggcgc tcgcgatcgt cgaagccgtc gcgcggcggg ccaccggcac
     2821 cgaccatgat ggcgcccgag tcgcggccgc tccgcgagag cttgcccttg tagatcttgt
     2881 ggtcgaggtt ctcgccgccg ttgcccgcgg cctcgatcac gagcacgcca cgtgacgtcg
     2941 ccgcttcgat cgcgtcgaag ttgtcgtccc agtactccat cggcagatag cggccgcgtg
     3001 ggccggcgcc ctggagctcg atcaacagca cgtcgcctgc gcgcaactgc tcggcggctg
     3061 catcgagcgc ttcggccgta gggatgccgc cgatcgacga cgtgaacacg cgctcgacat
     3121 cgggcgcgat gccgacgacg cccttgccgt tgtctctacc gacgacctcg ccgagcaccg
     3181 cagtgccgtg cgcgcgccag tggatatcgt tgatcggctc acccgcgaca tggctgatgc
     3241 gatcgcccgg caggtcttca tgcttgctgt tccaaccgcc ctcgatatcg gcgaaccaga
     3301 tgccctgccc gcgcacgccg cgtcgccacg ctgccggcgc atcgatacca tgtggcgccg
     3361 gaccgaggta gccctgatac gactcgaacg acggcgtgtg gatcggacag ctctcgtcat
     3421 cggcctgcac cacgggtcgt cgcggcagtg tcaccgtcgg cggtacgaat gctgtctcga
     3481 cggccggatc gttcgcgagc tgcgcgagga tctgcttcgt gtagcgagtg tcgacctcga
     3541 tgcggaccca gcgatcgcgt ggatcgtcat aggcggcaac actctggtgc gcgtggcgga
     3601 acagcggccg caccgtgtac gcgtgcgcca tcgtctggta gcgaaccatg cccggccgat
     3661 acttgatgtg gatcggtgcg agccggatcg cctgcgatgt gcgcgcgggt tcgcgacaca
     3721 tgcgccagaa caagatgatc gcgagcaaca ggagcgcgat cgagaggtac cgcaggacgg
     3781 tgggaacgtc ggcgatccgg cgcattcccg aatcctacgt tgcccgaggg gatttgccag
     3841 cgctattggc agtactggac gtatgcggcg atgcgcgcgc gcaggcgcgc ctcgagcaca
     3901 gggcacgcgt aaccgagcgt atcggcgatg aggccggcct cgagccgata ggccgcgcac
     3961 ttctccttcg tccagtaatg cgggggctcg gataggttcg tgatgcgatc agcgagcttg
     4021 acgatcgcga tctcgcgcgg ctgctctttg atgcggcgca ggctgtcggc catctgctcc
     4081 ggcttcgtct cgagcttctg gttcttcgtc agcgcggaga caccggccgc aactgcatcg
     4141 ccgtagcgcg cgcgaatctc gtccagcgtg accttcgtgt cctcgacggt gtcgtggagg
     4201 agcgcgcagg tgacggcgag atcggcgtcg atgccgttca tcgccggcag cgcggcgatc
     4261 acttccgccg cgaccgatac aacgtgcacg agatagggca gctcgtggcc aggttccttc
     4321 tgcgcgttgt gcgcggcggc ggcgaaccgc atcgcatcga cgtagcgctc aggcgagtac
     4381 atggtcgcag cgtagcatca gtgcagcgct gcgataccgg cacgtgcagc tgcgaccacc
     4441 gacgcatccg catcgccaag tgcggattcg aaccacgcgc gcgccgctgc gttatctccg
     4501 cgttctgcga tgagccgcgc gagcatcacc gcagcacggc cgcggcgaat cgacgtcggt
     4561 gcgcgatcga taaacgcgac aagcgcgcgt tctgcctcgg tcttgcggcc agctttcgtc
     4621 aatgctgcag ctgcgaagta gcgtgcgtcg gcggcgagcg gatcatcgcc accggcatcg
     4681 gccgcagcaa gcagctcgcg agcggcttcc gcggacttgc cgctcttgag cagcgccatg
     4741 cccgcctgga agcgacgctc gacctcgttc gggcccgtcg caggtgcaac attctgcttc
     4801 gccggtgcaa cattatgctg cggtgaaact tgttgtacgg gagcggcggc accgccacgt
     4861 tccggtgcag ctgtcgcagt cgtcgcaggt tgcggttcct cggtacgggc ctcctgcaac
     4921 ggtgtttcct gcaccacgac atgctgtcgt gtcgcgaccg gcttccgttc gtcgacaatc
     4981 gcgacggcag gtgcgggcgc gacga
//
LOCUS       DASUFX010000421        20808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1597611, whole genome shotgun sequence.
ACCESSION   DASUFX010000421 DASUFX010000000
VERSION     DASUFX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20808
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597611"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VFV99_16795"
     CDS             <1..1300
                     /locus_tag="VFV99_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5061029.1"
                     /translation="MRAEKVGSETLLARIVAMVAEAQRSRAPIQKLADQVSAWFVPIV
                     ILIAIATFVVWSLVGPDPRMAHAIINAVSVLIIACPCALGLATPMSIMVATGRGAHMG
                     VLFRNAEAIEVMRQVDTLVVDKTGTLTEGKPQLVSVVPTTGDEASLLRLAASLERASE
                     HPLAAAIVAGATKRNVSLANVASFESVTGKGVRGVVDGKSVALGNRAMMTADGADTES
                     LADRAEALRADGQTVMYIAIDGKLAGLVGVADPIKDATPDAIRTLKAEGLRIVMITGD
                     SKTTANAVAKKLGVDDVLAEVLPDQKADHVARLQAEGRNVAMAGDGINDAPALARANV
                     GIAMGTGTDVAMESAHVTLVKGDLRGIARARALSRATMSNIKQNLFLAFVYNAAGIPI
                     AAGVFYPLTGWLLSPMLAALAMSLSSVSVITNALRLRKQR"
     gene            complement(1307..1672)
                     /locus_tag="VFV99_16800"
     CDS             complement(1307..1672)
                     /locus_tag="VFV99_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061030.1"
                     /translation="MRALVLCALVACGGEDSVFGPPTEATCPPNSPLTYDNFGAGFMA
                     SYCTRCHHSELTGAARMGAPSFHDFDTIFGIRAVSNHIDETTASGPASTNEGMPPDKP
                     FPSLTERKLLGEWIACGMP"
     gene            complement(1669..2103)
                     /locus_tag="VFV99_16805"
     CDS             complement(1669..2103)
                     /locus_tag="VFV99_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061031.1"
                     /translation="MRAVMLVALVAGCGGDDTPPLGSIDAAAAADASGVDAAPPRELI
                     METQPLQPGELVEGIMTGGPNDLALIHLEAPVTELDWNIHGHPDNTTVVVYEELNKMT
                     VDYAYTPAAQGDWFLLIRNSGPTNMDVKVRVGLYGGMTWQWQ"
     gene            complement(2100..2294)
                     /locus_tag="VFV99_16810"
     CDS             complement(2100..2294)
                     /locus_tag="VFV99_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061032.1"
                     /translation="MRALLLVACLAACGDNLKAKDDAGVSDAAPDAQALRHCLDRPTE
                     IVRPPNGQLPCDLLPPGFGQ"
     gene            complement(2294..2965)
                     /locus_tag="VFV99_16815"
     CDS             complement(2294..2965)
                     /locus_tag="VFV99_16815"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEX5061033.1"
                     /translation="MHKKFLLVALISGTAAAHPHIEGGPAFANVSGQLVTFGVSHGCS
                     GKDTVKIEVAIPTGVTGVRGLYGPFGAPKATKDANNNVTTVTWEKATYETDDNTYPEF
                     TLRLKVPDVPFTQLMFSVTQTCRDATGDTVVVWDKPDDTTGNAAPRLTVVPARAKGWN
                     KFMLPAGIAEADVPLYFGDAHIVWKGSAAYSPNAVVHAMIAMTPGVSVLSGDLAAGDE
                     IWVKY"
     gene            complement(2975..3415)
                     /locus_tag="VFV99_16820"
     CDS             complement(2975..3415)
                     /locus_tag="VFV99_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061034.1"
                     /translation="MRVILLLAFVVACGPSPEPTGTMCPEPDPLTLTYDNFGHEFMTK
                     YCTWCHSSELPRAQRNGAPVYHDYDSLLGVLETPDHIDEQAGFGPDAKNEFMPPDRCP
                     SVAGGALDKNCAKPTAEERTKLAEWIACERNRNHSFLDAGVDAP"
     gene            complement(3424..4179)
                     /locus_tag="VFV99_16825"
     CDS             complement(3424..4179)
                     /locus_tag="VFV99_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061035.1"
                     /translation="MRFWIAGTLLLSAGLARADHMHTVDHGDDGHAFGAGVTMIAASF
                     DQPLYTGNYQGVVPAVQWANERFAAGTSVALYRLEENGAEFYGFGDFVIHGQAMLIGN
                     HHRHAGVVAAVSAPIGDDRRGMGMGHPMAMPALFGTWMVERFAVAATAGYSRALGADS
                     GHDHGMWPIVEPMNMQEFTWSASGDYAFTPHVHGGARFSGGVPIGNGDNRIIGAARVA
                     WGSGRFSTAAELQAGIAGDPFTVRGVVSTAVSF"
     gene            complement(4194..4505)
                     /locus_tag="VFV99_16830"
     CDS             complement(4194..4505)
                     /locus_tag="VFV99_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061036.1"
                     /translation="MRLVLTAILFSTLAFACGHDDGEEAFDTYQDCFDDHTSGGEMLP
                     VKEAIVVCCLEHPIAGHTEVCGATTADCMTYLGTNLSATSATATEVSEACMDYVTQKG
                     M"
     gene            complement(4589..4921)
                     /locus_tag="VFV99_16835"
     CDS             complement(4589..4921)
                     /locus_tag="VFV99_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061037.1"
                     /translation="MTAAATFMVAIVLAICVPVSQLRTVATKTECCCPDPDHCKCPDH
                     KPDKSNQSQMRACHKTSQQFVSPEAPAFEAIEIAIVNLPAPVAIAPTFTHAAPHPAPA
                     PQRPDAPS"
     gene            complement(5130..6446)
                     /locus_tag="VFV99_16840"
     CDS             complement(5130..6446)
                     /locus_tag="VFV99_16840"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5061038.1"
                     /translation="MANLHAIVLAADPLSQVRIAGLSACERAVRVAKRIGAESVHVVD
                     DDNSRASLESWWTARSGKLLVIRANQLVHTPLVDKLIASGGDAIAVVPDNPAVNDLTP
                     GEFAGAFVVGGGAATDVVAALARGETDVAIVAKFSEPQRVPHGAIARAPIATPDERRA
                     ATRLLHRILIKPQDNAITRYAYRPVSMPLSKLLVWTPITPNQISYFVAMMVAFGCWLT
                     AHADFDLVLLGTVVVLLASYIDCCDGEIARLKLLSSRYGAWVDTVVDELSSVGYMVAL
                     GWHCHLAFGPNYFGNLGFDPWLAATAVGVVTYLWSIYCIYYNIIVAVGSANSQDYVGR
                     FEVVPGKQPNSMRLAPAATKVITTKEPLPQPLELILTYLPYVVRRDFICWAAVAFAAL
                     HATHAGFGTLVLGGLISFVILSIDHMKLRSLRRSVMRRGLLLEAPR"
     gene            6639..7406
                     /locus_tag="VFV99_16845"
     CDS             6639..7406
                     /locus_tag="VFV99_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX5061039.1"
                     /translation="MKAVILAAGCATRLRPHSDDTPKTLLPVGGVPILRRTITSLLRC
                     GFDQFVIGTGYLEHMVRQAVEAWFPTLDVTYVSNKDFRSTNNAYSLLLCRPHVENDSF
                     ILLDGDVVFDLGVIEELVERGPDCLAVRSVGEIGLEEVKVTADVEDRVLAIGKHVPVR
                     SAMGESVGIELFSAASSRRLFAALHNRVTEQGLVNEYYEASFQQIIDDGTTLYGVDIG
                     SMYATEIDTIDDLLAANARLAARPAFDISVGAVRLAV"
     gene            7472..8251
                     /locus_tag="VFV99_16850"
     CDS             7472..8251
                     /locus_tag="VFV99_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061040.1"
                     /translation="MKGLLYLVLLLGACATASREDVVGERPDGGPPGGGGSGKDASVI
                     PPVDAAPLPDAPPGQQQVTLEQTNNNTMAAGAAIACSSSIAGIPVGTRDNSWYRVFKL
                     SDYGITGAFTLQRITFYTDYAYAGSGTSQPASLKIGTYSGTPDTDTLTTGSIANLMTV
                     PIMIPDADSSGGTPPPVITDVTVNIPANSNLIVELALPDGYADGNFYYIGVSSGGEAH
                     KGYIRATPSGCNMTMPKSLTSATGLNQPTNSILLSVTGTKM"
     gene            8303..8953
                     /locus_tag="VFV99_16855"
     CDS             8303..8953
                     /locus_tag="VFV99_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061041.1"
                     /translation="MGSLRWLSCVVLVACGPTEVTAPAGGGENFENPYDVTRTQLAQT
                     EDMTIAPQLSLACSDANGTKENSWYRMFPLADYGVDKPLTIHRVNFGVQTAIGDQRVK
                     VSIGTYAGDAGSVELDPTKIDVLGLTTIPVPEGASQMLQANFPAIEVPHNANLIVEIK
                     SEGTGDGRFFYLGATRGAETIPGYLRAPTCKTPNPVMTSALGFSQTHVIIAVSGAY"
     gene            8975..9715
                     /locus_tag="VFV99_16860"
     CDS             8975..9715
                     /locus_tag="VFV99_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061042.1"
                     /translation="MRWLALVLACGCASAGSQDQFGGVDGNNQPQPDGQQNPMIDSSM
                     GGMDGSGSNMPTSHTLTQTTSQTLKAANTIACGASGTTAANNWYRVFDLPAMGITTSI
                     AVTQVSFQVEHCHATANNGCTVAVRVGTYSATPGATLATANMTILSSNPTVTIPEIVE
                     SGTTTPGGTVNAPVSATIPAGSKLLVEVDAPDGTGVYAFYMGSNDGGESGFGYIMAPT
                     CSVSTPTNISTLGTPAPRHLLLTVTATY"
     gene            complement(9699..10331)
                     /locus_tag="VFV99_16865"
     CDS             complement(9699..10331)
                     /locus_tag="VFV99_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061043.1"
                     /translation="MDPKFAEVRRLLDRYIVEIVETYDFCPWAKAARTGGEIAVEVLW
                     DTPALDTWVGAAQRLLGRAETRVAMVVAPELGATPAELRLIRDRVAARVPTAGVAEFH
                     PDATIDLATPARLVPFLRRSPDPLLQLVPLELLDRVRAHTAVVDITEQAQMLGGLAAP
                     LRGDIADTLAETNHQRVTADHAAIAATLDDIAADRRRSYPRAGIAINTSR"
     gene            10371..11369
                     /locus_tag="VFV99_16870"
     CDS             10371..11369
                     /locus_tag="VFV99_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061044.1"
                     /translation="MKLAIVFVLATTVTAYADDRCATGVELAKQAHDLPRAALYLESC
                     TDDDSVRVRNSVAHKLEDTQLSHVTVVSMPEGIEGETTAMPGEKFTTPATIWTKAGTY
                     KITVGGQTVEKVVEPHSRTTVIINVPPPPKPAKNGSVSFEEEPEQHQGTPAAVKHGTM
                     LPKKYLKPSAPTGEQIDDPLPYHTDSTLAWRLGVRATGGIADRTNAGIAPSVGLAVLA
                     ARPLDGPIMLATRLDYAHRKLDTIGLEVGFAFLALERPSFVLSATAALRGEVRVQSKL
                     DQMDVNRAGIAGAAGLDLAVLSLPLAFGARVEQGFSELMPGVHDRAYLLEVGYDWR"
     gene            complement(11366..11713)
                     /locus_tag="VFV99_16875"
     CDS             complement(11366..11713)
                     /locus_tag="VFV99_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061045.1"
                     /translation="MIALERHDRDFMIFLNEVRRQKVDIAIPAGVVAQAWRDGRRQAR
                     IARFLTAPQVRVEVLDEFRARAAGQLCGVTGTADVVDASVVLCARENKHAVATSDPDD
                     LRRLDPTLDLFVV"
     gene            complement(12081..13409)
                     /locus_tag="VFV99_16880"
     CDS             complement(12081..13409)
                     /locus_tag="VFV99_16880"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5061046.1"
                     /translation="MRAVIVVVALLGAARAQALADDAKPLVGVRVRGDSKTTNETAMR
                     LARVSLGDPISVDMLPELQAALLSSELFKSVTVWLEEAPGGVIIVATLEDKWSWVAAP
                     TLYLLPDNWSVGVGYAENNLFGQDKKLLLYGQVGNINSLFFGTYLDPAFRATKLQLRF
                     DIYFVRRLIQEYVNDPAQPTDYAIARETLWTFADLGVLFGWRFYWWLSADTRLKPGYS
                     QFSQFTTRPERDGWDTSVQTRLTVDHRQHRYGVTWGPYAQLITDVSVPGLSDYNYQVA
                     AARAYYSWRFFPEDPHAAGFLGRFGREHQLEFRTSLGIGHHLPLHEELTLGGVIDLRG
                     YPVDMFRGDRRAAGRAEYSMQLAKWWMFSFRAIGFFDAGYVGFHARDTQTREYLPTQL
                     GDAHWIRSDVGGGVRIYVGNVVLPLLGLDYAYGLEGERHEVYFEIGLTDF"
     gene            complement(13422..14582)
                     /locus_tag="VFV99_16885"
     CDS             complement(13422..14582)
                     /locus_tag="VFV99_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5061047.1"
                     /translation="MRFTDEHQQLRRTVRDFVEKEINPHVDKWERDGAFPAHEVAKKA
                     GRLGLLGVNKPEAYGGMGLDYSYQMVVTEELGTCASGSIPMALGVLTDMATPALSRWG
                     THELKQEFLAPVIAGEQIASIAVSEASGGSDVAQIKTTARKDGADYVINGSKMWITNA
                     AQADWLCTLVCTSEGAPHMNKTLVVIPAKTKGVEIGAKIDKLGMRASDTCPVFFENVR
                     VPQRNRIGEEGMGFMMQMVQFQEERLCGAVGTLRGMEGLIDATIAYCKERHTFGQPLI
                     DNQVIHFRMAEMKTEVEALRSLIYRAVEEYVAGTDVTMLASMAKLKAGRLVREVSDTC
                     LQYFGGMGFTWDNVAARAYRDTRLLSIGGGADEIMLGIICKLMNILPGKKKK"
     gene            14774..15400
                     /locus_tag="VFV99_16890"
     CDS             14774..15400
                     /locus_tag="VFV99_16890"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5061048.1"
                     /translation="MGMAAASWTAAASPMPLPAESVATLFATRAATPVDVDLYDENDH
                     QKGHIAIWRDGSVDEDTRVIVKRMFRCRKTHREAMIHHTTLAMLADVAERYEGKTIEY
                     VSAFRVGHAESRTSPHRDARAIDFRIRGTNLREIRDYLWRTYTEVGVGWYPKGQYIHI
                     DARPTMTDMSWTFVNGTNRYHPYWAELARKPELETDAAQMAHARRSGS"
     gene            15413..16324
                     /locus_tag="VFV99_16895"
     CDS             15413..16324
                     /locus_tag="VFV99_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX5061049.1"
                     /translation="MISRFAPSTTGEAHPGTLLSGLLVWLDARTRKGQALLRLEDLDR
                     TRVKEAWSDQLLEATRWLGLDWDAVIVQSVRNASHERALDQLEALGRLYPCTCSRADR
                     AGGRRAPDGSWAYENTCRGKLLPDGGWRAVDGSVRVQLPDERIELVDDGGLDLSQTPA
                     HDIGDPIVRRRDGVIAYQLAVVIDDADEGITDVVRGRDIAPSTATQVMLQRLLGLPTP
                     RYRHHFLLLEHSGDKLAKLHGSIPWSVVRAHYSGDAMCGALAHAAGLLATPRPCTPSE
                     LVHTFTWSRVPTADRLTHWRDNKLVFE"
     gene            complement(16325..17665)
                     /locus_tag="VFV99_16900"
     CDS             complement(16325..17665)
                     /locus_tag="VFV99_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061050.1"
                     /translation="MPVIARDAVASDHLVFAWPFPIGPEEYEVSCADDELVVMCPSFM
                     VGDRLGPGRHRWRTPDPMRPVSAYFVLTAPVEVSYDMITLFQLPTTGQHVRIRANGSL
                     QVRCMDPALLVAQFVGLPFDAVNDGVLRSVSRSIERMLARLLTRRVVMAGTPNAVTDP
                     QMLGNIIEELVAYNPAAGAVFGVELVRMGHLSIIADDGSRPWQQMTAQGSNPRLPANG
                     DTRRDQQHLVETARAPRPEPSNLLETVRQVPPPAAKPPASTPKPRAQTHPQVEASGEI
                     VVKPKEKPLREEKQTAPGHAAAAAPITSAGASASPAPDPHRVIQTPVPPSSPRRAGAV
                     EVQAADDKTDPGSEAPKSTANDAKITPVEGHRGAIMGIGMSAIGSGGASSASTSGEIG
                     GSKKIAPGGRVLVPGPGGLMQSATVRQLLQGYYELEVGGSGETIWVPINGVVPE"
     gene            complement(17761..19074)
                     /locus_tag="VFV99_16905"
     CDS             complement(17761..19074)
                     /locus_tag="VFV99_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5061051.1"
                     /translation="MGTAILIVLAFLVGNGLFAGAEIAILSVRKTRLREFIRRKDKRA
                     LAVKKLRDQPERFLATVQVCITTFGTAASVYGGAELEPTIAHALTQFGLGHGTALVLV
                     VIMIVYLELVIGELVPKSLALRYSDRYSFFAGRPLTFISQLLRPVVWTLTSASNILLR
                     FFGDKTTFTETRLSRDELQQLVEEAAKTGSVDPRASEIASRALGFGEVTVAEVMVPRN
                     RVVALRRGAPPEEVQRVILEEGHSRMPVFDQSIDNIVGYVVARDVLALAWEKGLVVLE
                     DILRPPHKVPESTRALDLLRELQRRRTQMAVVVDDNGGLSGLVTAEDLIEELVGDIMS
                     EDDVVEQYFVREPQGTILVQGWAAVRKVNRDLHLDLPVGKDRTTIAGLCMSLAQAIPQ
                     AGEKLTTDNGTVLEVIEASPRRVRRVRIHPVAETDMDEGLEDAGE"
     gene            19113..19616
                     /gene="greB"
                     /locus_tag="VFV99_16910"
     CDS             19113..19616
                     /gene="greB"
                     /locus_tag="VFV99_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX5061052.1"
                     /translation="MSTRDRPQPPPKKSYITPDGAKKLRAELDQLWTVERPRVTQEVA
                     DAAAQGDRSENAEYIYGKRRLREIDRRVRFLSKRLDAVTIVSEPPTDPSRAFFGAYVT
                     LEDDDGNEVTYRIVGPDESDVDKGWISMEAPVAKALFGKREGDDVTVRRPKGDITYTI
                     VSVRYDT"
     gene            19620..>20808
                     /locus_tag="VFV99_16915"
     CDS             19620..>20808
                     /locus_tag="VFV99_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5061053.1"
                     /translation="MAAPAPRQPDAVPEPVDDKPSRAIAIQLPATMQVYARSPLRWRV
                     GCDATVLRDGAATYHAMLEAIQGAKQSICLETYILASDTTGDRFKLALVERARAGVKI
                     RLIYDAVGSFGISDAWVDDLRTAGIEVIDFNPISPWRRRFRLSHRDHRKLLIVDNEIG
                     FTGGLNIANDYASVDEGGVGWHDMHCRVKGPIVYDMARLFRRNWIRSGGDAYPPVPRP
                     DEGSGQGASFIRLLENTKRRNKTTFRRAYLHVIRAARSLVRIENAYFLPDRGVRRALV
                     RAAMRGVDVQIIVPGHSDIRLIEWASLYVLKNLTRRGIKVWRWRGVMMHAKTATVDAI
                     WSTIGSYNFDSQSRFNNLEVTVEILDPDVGNTLVKEFDADMPNCDAYDETVWRQLPWW
                     KKAL"
BASE COUNT         3693 a         6864 c         6705 g         3546 t
ORIGIN      
        1 catgcgcgcc gagaaggttg gctccgagac gctgctcgct cggatcgtcg cgatggtcgc
       61 cgaggcgcag cgcagtcgtg caccgatcca gaagctcgcc gaccaggtct ccgcttggtt
      121 cgtgccgatc gtgatcctga tcgcgatcgc gacgttcgtc gtgtggtcgc tcgtcggtcc
      181 cgatccgcgg atggcgcacg cgatcatcaa cgcggtgtcc gtgctgatca tcgcgtgccc
      241 gtgtgcgctc ggtctcgcga caccgatgtc gatcatggtc gcgaccggtc gcggcgcgca
      301 catgggcgtg ctgttccgca atgccgaagc gatcgaggtc atgcggcagg tcgatacgct
      361 cgtcgtcgac aagacgggca cgctgaccga aggtaagccg cagctcgtct ccgttgtccc
      421 gacaacgggt gacgaagcat cgctgcttcg cctcgcggct agcctcgagc gtgcgagcga
      481 gcatccgctg gccgcggcga tcgtcgccgg cgcgaccaaa cgtaacgtct cactcgcgaa
      541 cgtcgcgagc ttcgagtccg tcaccggcaa aggtgtgcgt ggcgtcgtcg acggcaagag
      601 cgtcgcactc ggcaaccgcg cgatgatgac cgccgacggc gctgacacgg agtcgctcgc
      661 cgaccgcgcc gaagcgctac gcgccgacgg tcagaccgtc atgtacatcg cgatcgacgg
      721 caagctcgct ggtctcgtcg gtgtcgcgga tccgatcaag gatgcgacac ccgatgcgat
      781 ccgcacgctc aaggccgagg gtctacgcat cgtgatgata accggcgaca gcaagacgac
      841 ggcgaacgcg gtcgcgaaga aactcggcgt cgatgacgtg ctcgccgaag tgctgcctga
      901 tcagaaggcc gatcacgtcg cgcggctgca ggccgaggga cgcaacgtcg cgatggccgg
      961 cgacggcatc aacgacgcgc ctgcgctcgc gcgcgccaac gtcggcatcg cgatgggcac
     1021 cggcaccgac gtcgcgatgg agagcgccca cgtgaccctc gtcaagggtg acctgcgtgg
     1081 tatcgcacgc gctcgcgccc tgtcgcgagc gacgatgtcg aacatcaagc agaacctgtt
     1141 cctcgcgttc gtctacaacg ccgccggcat tccgatcgcc gctggcgtgt tctacccgct
     1201 caccggctgg ctgctctcgc cgatgctcgc agcgctcgcg atgagcctgt cgtcggtgtc
     1261 cgtcatcacg aacgcgctgc gactgagaaa gcagcggtga gtttggctac ggcatcccgc
     1321 aggcgatcca ctcgccgagc agcttgcgct ccgtgagact tggaaacggt ttatcgggcg
     1381 gcatgccctc gttcgtcgac gctgggcccg aggcggtcgt ctcgtcgatg tgattcgaga
     1441 ccgcgcgaat gccaaagatt gtgtcgaagt catggaacga cggagcaccc attcgcgctg
     1501 cccccgtgag ctcgctatgg tgacagcggg tgcagtaact ggccatgaag ccggcgccga
     1561 agttgtcata cgtcagcggc gaatttggcg gacacgttgc ttcggtcggc ggtccgaaca
     1621 cggagtcctc gccaccgcac gcgacgagcg cgcacaatac gagtgctctc attgccattg
     1681 ccatgtcatt cccccgtaca gcccgacgcg aaccttgacg tccatgttcg tcggcccgct
     1741 gttgcgaatg agcaggaacc agtcgccctg cgccgccggc gtgtacgcgt agtcgaccgt
     1801 catcttgttg agctcttcgt agacgacgac agtcgtgttg tctggatggc cgtggatgtt
     1861 ccagtccagc tcggtgaccg gcgcctcgag atggatcagc gcgaggtcat tcggcccgcc
     1921 ggtcatgatg ccctcgacga gctcgcccgg ctgaagcggc tgcgtctcca tgatcaactc
     1981 gcgcggcggg gcggcgtcga caccgctcgc gtccgccgcg gcggccgcat cgatcgagcc
     2041 gagcggcggg gtgtcgtcac cgccgcagcc ggcgacgagc gcgacaagca tcaccgctct
     2101 cattggccga accccggtgg caacagatcg cacggcaact ggccgttcgg cggccgcacg
     2161 atctccgtcg ggcgatcgag acagtgtcgc aatgcctgcg cgtcgggcgc cgcgtcgctc
     2221 acgcccgcgt cgtccttcgc tttaaggttg tcgccgcacg ccgcgaggca tgcgacgagg
     2281 agcagcgcac gcatcagtac ttcacccaga tctcgtcgcc ggccgcgaga tcaccagaga
     2341 gcacgctcac gccgggtgtc atcgcgatca tcgcgtggac gaccgcattc gggctgtacg
     2401 cggcgctgcc cttccacacg atgtgcgcgt cgccgaagta cagcggcacg tcggcctcgg
     2461 cgatgcccgc gggcagcatg aacttgttcc agcccttcgc gcgcgcggga accacggtca
     2521 gccgtggcgc cgcattgccg gtcgtgtcgt ccggtttgtc ccagacgacg acggtgtcgc
     2581 ccgtcgcgtc gcggcacgtc tgcgtgacgc tgaacatgag ctgcgtgaac ggcacgtcgg
     2641 gaactttgag ccgcagcgtg aactcggggt acgtgttgtc gtcggtctcg taggtcgcct
     2701 tctcccacgt caccgtcgtg acgttgttgt tggcgtcctt ggtagccttc ggcgcgccga
     2761 acggaccgta caggccacgc acgccggtga cgccggtcgg aatcgcgacc tcgatcttca
     2821 cggtgtcctt gccggagcag ccgtggctaa cgccaaaggt gacgagttgg cccgacacgt
     2881 tcgcgaacgc ggggccgccc tcgatgtgag ggtgagcagc tgctgtgccg ctgatgagag
     2941 cgaccaacag aaacttcttg tgcatagaaa actcctaagg agcgtcgacg ccagcgtcga
     3001 ggaaagagtg attgcggttg cgctcacacg cgatccactc ggcgagcttc gtgcgctcct
     3061 cggcggtcgg tttcgcgcag ttcttatcga gtgcaccacc cgcgacgctg ggacagcggt
     3121 cgggcggcat gaactcgttc tttgcatccg ggccgaagcc cgcttgttcg tcgatgtgat
     3181 ccggcgtctc gagcacgccg aggagcgagt cgtagtcgtg atagaccggc gcaccattgc
     3241 gctgcgcacg cggcaactcg ctcgagtgac accacgtgca gtacttcgtc atgaactcgt
     3301 ggccgaagtt gtcgtacgtg agcgtcagcg gatccggctc gggacacatg gtccccgtcg
     3361 gttccggcga cggcccgcac gcaacgacga aagcgagtaa caagatgacg cgcatacgtg
     3421 ctcctagaaa ctcacggcgg tcgagaccac gccgcgaacg gtgaagggat cgccggcaat
     3481 gccggcctgc agctctgccg ccgtcgagaa gcggccactt ccccacgcca cccgcgccgc
     3541 accgatgatg cgattgtcgc cgttgccgat cggcacgcca ccggagaacc gagcgccacc
     3601 gtgaacgtgc ggagtaaacg cgtagtcgcc agaagcgctc cacgtgaatt cctgcatgtt
     3661 catcggctcg acgatcggcc acatcccgtg atcgtgaccg ctatcagcac cgagtgcacg
     3721 gctatagccg gcagtcgccg ccaccgcaaa acgctcgacc atccacgtgc cgaacagcgc
     3781 gggcatcgcc atcggatggc ccatgcccat gccgcggcga tcgtcgccaa tcggcgccga
     3841 caccgctgca acaacaccag cgtgacggtg atggttgccg atcagcatcg cctggccgtg
     3901 gatgacgaaa tcgccgaagc cataaaactc ggcgccgttc tcctccagcc gatacaacgc
     3961 gacgctcgtg ccggccgcaa agcgctcgtt cgcccactgc accgcgggca cgacgccctg
     4021 atagttgccg gtgtagagcg gctgatcaaa cgacgccgcg atcatcgtca cgccagcacc
     4081 gaacgcatgc ccatcgtcac cgtggtcgac ggtgtgcatg tgatcggcgc gcgccaagcc
     4141 tgcgctcagc agtagcgtgc cggcgatcca gaaccgcatg gacatcgtac gagctacatg
     4201 cccttttggg tgacgtagtc catacatgcc tcgctcacct ccgttgccgt cgcgctcgtc
     4261 gcggataggt tggtgccgag gtaggtcatg caatcggccg tcgtggcgcc gcacacttcc
     4321 gtatggccgg cgatcggatg ctcgagacag cagacgacga tcgcttcctt gaccggcagc
     4381 atctcgccgc cgctggtgtg atcgtcgaag cagtcttgat acgtgtcgaa cgcctcttcg
     4441 ccgtcgtcgt ggccacaggc gaaagcgagc gtggagaaca gaatcgcagt gagaaccaaa
     4501 cgcataacaa acctcgtagc gagaaatgat caaatgcacg cgagcagagc tcgcgcacgc
     4561 ggacaacggt tggatacgtc gtcgctcgct aagaaggcgc gtcgggccgt tgcggtgcag
     4621 gagcaggatg aggagctgcg tgcgtgaacg tcggcgcgat ggcgaccggc gcgggcaggt
     4681 tcacgatcgc gatctcgatc gcctcgaatg caggcgcttc cggcgagacg aactgctgcg
     4741 atgtcttgtg acacgcgcgc atctgcgact ggttgctctt gtcgggcttg tggtcgggac
     4801 acttgcagtg atccggatcc ggacagcagc actcggtctt cgtcgccacc gtgcgcagct
     4861 gcgagacggg cacgcagatc gcgagcacga tcgccaccat gaaggtagcg gcggcagtca
     4921 cgacgcggcg caagctcacc cgcacatttg ggcattaggc cgccggagtg tcaacgaaag
     4981 cccgtcatca cacggcataa ctgtggtgag gtccgcgttg cgcgctcgat gagagtggcg
     5041 ctcattgctg cgatcgtgac gcatgtcgcg gtgatcgctg cgacactcgt acctgtaaca
     5101 aatcagccgt agtgtccgtc gcgcgagcgc tagcgcggcg cttcgagcag tagcccgcgg
     5161 cgcatcaccg agcggcgcag cgagcgcagc ttcatgtgat cgatcgacag gatcacgaac
     5221 gagatcaaac cgccgagcac gagcgtgccg aagcccgcgt gcgtggcatg cagcgctgcg
     5281 aacgcgactg cggcccagca gatgaagtcg cggcgcacga cgtacggcag atacgtcagg
     5341 atgagctcga gcggctgcgg cagcggttcc ttcgtcgtga tcacttttgt cgccgcaggt
     5401 gcgaggcgca tgctgttcgg ctgcttgccg ggcacgacct cgaaacgacc gacgtagtcc
     5461 tggctgttcg ccgaaccgac cgcgacgatg atgttgtagt agatgcagta gatcgaccac
     5521 agatacgtaa cgacgccgac ggctgtcgct gcgagccacg gatcgaagcc gaggttgccg
     5581 aagtagttcg gtccgaacgc gaggtggcag tgccagccaa gcgcgaccat gtagccaacc
     5641 gatgacagct cgtcgacgac cgtgtcgacc caagcgccgt agcgcgacga caacagcttg
     5701 aggcgcgcga tctcgccgtc acagcaatcg atgtacgacg cgagcaacac gacgacggtg
     5761 ccgagcaaca cgagatcgaa atccgcgtgc gcggtcagcc agcagccgaa cgcgaccatc
     5821 atcgcaacga agtacgagat ctggttcggc gtgatcggcg tccacacgag cagcttcgac
     5881 agcggcatcg acaccggccg atacgcgtag cgcgtgatcg cgttgtcctg cggcttgatc
     5941 aagatgcgat gcaacaggcg cgttgccgcg cgtcgctcgt cgggagtcgc gatcggagcg
     6001 cgtgcgatcg cgccatgcgg aacgcgctgc ggctcggaaa atttcgcgac gatcgctaca
     6061 tccgtttcgc cgcgcgcgag cgcagcgacg acatcggttg cggcgccgcc accgacgacg
     6121 aatgcacctg cgaactctcc gggcgtcaga tcattgacgg cagggttgtc gggcaccacc
     6181 gcgatcgcat caccgccgct cgcgatgagt ttgtcgacga gcggcgtgtg cacgagctgg
     6241 ttggcgcgga tgacgagcaa tttgcccgaa cgcgcggtcc accacgactc gagggaagca
     6301 cgcgagttgt cgtcgtcgac aacgtgtacc gactccgcgc cgatgcgttt tgcaacgcgc
     6361 accgctcgct cgcacgcgga caagccggcg atccgtacct gcgagagcgg gtcggcagct
     6421 agcacgattg cgtggaggtt cgccatggtc cgaccgcact agcttctaca gaatgcgagc
     6481 cctcttcgcc agtggcgcgt tgatcgttgc cgggtgacag gatcttgtca gcacgaaggc
     6541 gcttcgcgcg tggacctgtc aaaagttgaa caggtcagag ggtcaactgc ctgaaagcca
     6601 tgcgcgcata tcgtttgcag aaggccggtc tctaaatcat gaaggcagtc atcctagccg
     6661 cgggctgtgc gacgcggctg cgtccccact ccgacgatac gccgaagacc ctcttgccgg
     6721 tcggcggcgt acccatcctg cggcggacga ttacgagcct gctccgctgt ggcttcgacc
     6781 agttcgtcat cggtactggc tacctcgaac acatggttcg ccaagcggtc gaggcgtggt
     6841 ttcccacgct cgacgtcacc tacgtgagca acaaggattt tcgcagcacg aataacgctt
     6901 actcgctcct gctctgccgc ccgcacgtcg agaacgacag cttcatcctg ctcgatggcg
     6961 acgtcgtatt cgatctcgga gtcatcgaag agctggtcga gcgcggcccc gactgcctgg
     7021 ccgtgcgctc ggttggcgaa attggtctcg aggaagtgaa ggtcactgca gacgtcgagg
     7081 accgcgtgct cgcgatcggc aagcatgtcc cggtgcgctc ggcgatgggt gagtcggtcg
     7141 gtatcgagct gttcagcgcg gcgtcgtcgc gccggctgtt tgcagcactc cacaaccgcg
     7201 tcaccgagca agggctcgtg aacgagtact acgaggcttc atttcagcag atcatcgacg
     7261 acggtacgac tctctacgga gtcgacatcg gttcgatgta tgcgaccgaa atcgatacga
     7321 tcgacgacct gttggcggcc aatgcccgcc ttgcggcgcg cccggcgttc gacatcagcg
     7381 tcggtgcggt ccggctagcg gtctagacgc cccaccgtcg gtcagcagtt ctcagttgtc
     7441 ctcgggtact tagtcgggca acgtggccct catgaagggg ctgctgtacc tggtcctgtt
     7501 actcggcgcg tgtgcaacgg catcgcgcga agacgtagtc ggcgaacgtc cggatggcgg
     7561 accgccgggc ggcggcggca gcggcaagga cgcgagcgtg atcccgccgg tcgatgcagc
     7621 accactgccc gatgcgccac cgggccagca gcaagtcacg ctcgagcaga caaacaacaa
     7681 cacgatggcg gccggcgccg cgatcgcatg tagctcgtcg atcgccggca tcccggtcgg
     7741 cacgcgcgac aacagctggt atcgcgtctt caagctcagc gactacggca tcacgggcgc
     7801 tttcacgctc caacgcatca cgttctacac ggactacgcg tacgccggca gcggcacgtc
     7861 gcagccggcg tcgctcaaga tcggcacgta ctcgggcacg cccgacaccg acacgctgac
     7921 gacgggatcg atcgccaact tgatgacggt gccgatcatg attcccgacg ccgactcgtc
     7981 cggcggcact cccccgccgg tgatcacgga cgtcaccgtc aacatccctg caaactcgaa
     8041 cctgatcgtc gagctcgcgc ttccggatgg ctacgccgac ggtaacttct actacatcgg
     8101 ggtgagctcc ggcggcgaag cccacaaggg ctacatccgc gcgacgccgt cgggctgcaa
     8161 catgacgatg ccgaagtcgc tgacgagcgc gaccgggctc aaccaaccga cgaactcgat
     8221 cttgctgagc gttaccggca ccaagatgtg agtaggagat gtcgggttgc gacccgacgc
     8281 aggcgcgcgg caccatggct agatgggctc tttgcgctgg ctctcatgcg tggtgttggt
     8341 ggcgtgtgga ccgacggagg tgacggcacc ggcgggcggt ggcgagaatt tcgagaaccc
     8401 gtatgacgtc acgcgcacgc agctcgcgca gaccgaggac atgacgatcg cgccgcagct
     8461 gtcgctcgcg tgctcggatg cgaacggcac gaaggagaac agctggtatc gaatgttccc
     8521 gctcgccgac tacggggtcg acaagccgct gacgatccat cgcgtcaact tcggcgtgca
     8581 gaccgcgatc ggcgaccaac gtgtgaaggt gtcgatcggc acctacgccg gagacgccgg
     8641 atcggtcgag ctcgacccga ccaagatcga cgtcctcggc ctgacgacca tcccggtccc
     8701 ggaaggcgct tcgcagatgc tgcaagcgaa cttcccggcc atcgaggtcc cgcataacgc
     8761 gaacctcatc gttgagatca aaagtgaggg gaccggggac gggcggttct tctacctggg
     8821 tgcgacgcgg ggcgccgaga cgattcccgg ctacttgcgc gcccctacgt gcaagacccc
     8881 gaacccggtc atgacctctg cgctgggctt ctcccagacc catgtgatca tcgctgtatc
     8941 cggcgcctac taagtttgat tagtatcgcg gcgaatgagg tggctggccc tagtgctcgc
     9001 ctgcggctgc gcgtccgcag gatcgcagga tcaattcggc ggcgtggacg gcaacaacca
     9061 gccgcagccg gatggccagc aaaatccgat gatcgattca tcgatgggcg gcatggacgg
     9121 ttccggcagc aacatgccga cgtcgcacac gctgacccag acgacgagtc agacgctcaa
     9181 ggcggccaat acgatcgcgt gcggcgctag cggaaccacc gcagcgaaca actggtatcg
     9241 cgtgttcgat ctcccggcga tgggcatcac gacgtcgatc gcggtgacgc aggtgtcgtt
     9301 ccaggtcgag cactgtcacg cgaccgcgaa caacggctgc acggtcgcag tgcgggttgg
     9361 cacgtactcg gcgacacccg gcgcgacgct cgcgaccgcg aacatgacga tcctctcgtc
     9421 gaatccgacc gtgacgattc ccgagatcgt cgagtccgga acgaccaccc cgggtggcac
     9481 cgtcaacgca ccggtcagcg cgaccatccc cgcgggctcg aagctgctcg tcgaagtcga
     9541 cgcgcccgac ggcacgggcg tctacgcgtt ctacatgggc agcaacgacg gcggtgagag
     9601 cgggttcggc tacatcatgg ctcccacgtg ctcggtcagc acgccaacga acatcagcac
     9661 gctcggcacg ccagcgccgc gccacctgct gctcacggtc accgcgacgt attgatcgcg
     9721 atgcccgcgc gcgggtagct gcgacggcga tcagccgcga tgtcgtcgag cgtggccgcg
     9781 atcgcggcgt ggtcggccgt cacgcgctga tggttcgtct cggcgagcgt gtccgcgatg
     9841 tctccgcgca gcggcgcggc aagtccgccg agcatctgcg cctgctcggt gatgtcgacg
     9901 actgcggtat gcgcacgcac gcgatcgaga agctcgagcg gcacgagctg cagcaaggga
     9961 tccggcgagc ggcggaggaa cggcacgagc cgcgctggcg tcgcgagatc gatcgtcgcg
    10021 tcggggtgaa actcggcgac gcctgcggtc ggaacccgcg ccgcgacacg atcgcgaatc
    10081 agccgcagct cggccggtgt cgctccgagc tcgggcgcga cgaccatcgc gacgcgagtt
    10141 tctgcgcgcc ccagtaaccg ctgggctgcg ccgacccagg tgtcgagtgc cggcgtgtcc
    10201 cacagcacct ctaccgcgat ctcgccgccg gtgcgcgccg cctttgccca cgggcagaag
    10261 tcataggtct cgacgatctc gacgatgtat ctatcgagga gccgccgcac ctcagcaaac
    10321 ttcgggtcca cgcaaccatt ctagagtttg cgtgtcatct tgagggagtc gtgaagctcg
    10381 ctatcgtctt cgtcctcgcc acaacggtca ccgcctatgc ggacgaccgc tgcgcgaccg
    10441 gcgtcgagct cgcgaagcaa gcgcacgatc tgccgcgcgc agcgctctac ctcgagagtt
    10501 gcaccgacga cgatagcgtc cgcgtgcgca acagcgtcgc gcacaagctc gaagacaccc
    10561 agctgtcaca tgtcaccgtg gtctcgatgc ccgaaggcat cgaaggcgag accaccgcga
    10621 tgccgggcga gaagttcacg acgcccgcga cgatctggac gaaggccggc acctacaaga
    10681 tcacggtcgg cggccagacg gtcgagaagg tcgtcgagcc gcatagccgc acgaccgtca
    10741 tcatcaacgt gccgccgccg ccgaagcccg cgaagaacgg cagcgtgtcg ttcgaggaag
    10801 agccggagca gcaccaaggt acgcccgctg cggtgaagca cggcacgatg ttgccgaaga
    10861 agtacctgaa gccgagcgcg ccgaccggcg agcagatcga tgatccgctg ccgtatcaca
    10921 ccgactcgac gctcgcgtgg cgcctcggcg tccgcgcgac cggtggcatc gcggatcgca
    10981 cgaacgcggg catcgcgccg agcgtcggcc tcgccgtgct cgccgcgcgc ccgctcgacg
    11041 gaccgatcat gctcgcgaca cgcctcgact acgcgcatcg caagctcgac acgatcggtc
    11101 tcgaggtcgg gttcgcattt ctggcgctcg agcggccatc gttcgtgcta tcggcgaccg
    11161 ccgccctgcg cggcgaggtc cgcgtgcaat cgaagctcga tcagatggac gtcaatcgcg
    11221 ccggcatcgc gggcgccgca ggcctcgatc tcgccgtgct gtcactgccg ctcgcgttcg
    11281 gcgcgcgcgt cgagcaaggc ttctccgagc tgatgcccgg cgtgcacgac cgcgcgtacc
    11341 tgctcgaagt cggctacgac tggcgctaga ccacgaacag gtcgagcgtc ggatcgagtc
    11401 ggcgcaagtc gtccggatcc gacgtggcca cagcgtgctt gttctctcgt gcgcacagga
    11461 cgactgatgc atcgaccacg tccgccgtcc cggtcacgcc acacagctgg cccgccgccc
    11521 gcgcgcgaaa ctcgtcgagg acctcgaccc ggacctgggg tgcggtcaag aaccgcgcga
    11581 tccgcgcctg ccgccgaccg tcgcgccatg cctgagcaac tacccctgcc ggaatagcga
    11641 tgtcgacctt ctgtcttcgc acttcattca gaaagatcat gaagtcacga tcgtgccgct
    11701 cgagcgcgat caatgcaccg gtatcgagtg ttagtccgct catcgtgccg ctcgacgacc
    11761 gtgcttcgcg ttcatcaaga gcgccacttt cctgcgagcg cgctggcgtt cggccgcggt
    11821 gggtgggcct cctgcctgtt cgatccattc gtcgagcatc gcctcgagct catcacgctc
    11881 cgccttctgt tcgacagctt gggcgacgta cgcactcacg ctcgctgcct cccccgcttt
    11941 caccgcccga cgggccttcg cgacgagatg gctgggaacg cttacggcaa tcttttcgta
    12001 ccgagtcatg ccaccgcgtt tactcggtgc ctctgacgcc gaacgtacac gacgggtcat
    12061 accaccagta tgaccggggt ctagaagtcg gtcaatccaa tctcgaagta cacctcgtgg
    12121 cgctcgccct cgaggccgta ggcgtaatcg aggccgagca gcggcagcac gacgttgccg
    12181 acgtagatgc gcacgccgcc accgacgtca ctgcggatcc agtgcgcgtc gcccaactgc
    12241 gtcggcaggt actcgcgcgt ctgcgtatcg cgcgcgtgaa agccgacgta gccggcatcg
    12301 aagaatccga tcgcgcggaa cgagaacatc caccacttcg cgagctgcat cgagtactcg
    12361 gcgcgacccg ccgcgcgtcg atcgccgcgg aacatgtcga ccggatagcc gcgcagatcg
    12421 atcacgccgc cgagcgtgag ctcttcgtgc agcggcaagt ggtggccgat gccgagtgat
    12481 gtgcggaatt cgagctggtg ctcgcggccg aagcgaccca agaagccggc cgcgtgcgga
    12541 tcctcgggga agaagcgcca gctgtagtac gcgcgcgccg cggcgacctg atagttgtag
    12601 tcgctgaggc ccggcacgct gacgtcggtg atcagctgcg cgtagggtcc ccacgtcacg
    12661 ccgtagcgat gctggcgatg atcgaccgtg agccgggtct gcacgctggt atcccagccg
    12721 tcgcgctcgg gtcgcgtcgt gaattgcgag aactgcgagt agcccggctt caggcgagtg
    12781 tcggcgctga gccaccagta gaaccgccag ccgaacagca cgccgagatc ggcgaacgtc
    12841 cacagcgtct cgcgagcgat cgcgtagtcg gtcggctgcg ctggatcgtt gacgtactcc
    12901 tggatcaacc ggcgcacgaa gtagatgtcg aagcgcagtt gcagcttggt ggcgcgaaat
    12961 gccggatcga ggtacgtgcc gaagaacagc gagttgatgt tgccgacctg gccgtagagc
    13021 aggagcttct tgtcctggcc gaacaggttg ttctcggcgt agccgacgcc gaccgaccag
    13081 ttgtcgggaa gtaggtagag cgtcggcgct gcgacccacg accacttgtc ctcgagcgtc
    13141 gcgacgatga tgacgccgcc gggcgcttct tcgagccaga ccgtgaccga cttgaacagc
    13201 tcgctcgaca gcagcgccgc ctgcagctcg ggcagcatgt cgacgctgat aggatcgccg
    13261 agcgagacgc gcgcgagcct catcgcggtc tcgttcgtcg tcttgctgtc gccgcgcacg
    13321 cgcacgccga ccagcggctt cgcatcatca gccagagcct gggcccgcgc cgcccccagc
    13381 agcgcaacaa ctacgatgac ggcgcgcaaa cggcggtcag tttacttctt cttcttgccc
    13441 ggcagaatgt tcatcagctt gcagatgatg ccgagcatga tctcgtcggc gccgccgccg
    13501 atcgagagca ggcgcgtatc gcgatacgcg cgcgccgcga cgttgtccca cgtgaagccc
    13561 atcccgccga agtactgcag gcacgtgtcg gagacctcgc gcacgaggcg gccggccttg
    13621 agcttcgcca tcgacgcgag catcgtgacg tcggtaccgg cgacatactc ctcgaccgcg
    13681 cgatagatga gcgagcgcag cgcttcgacc tcggtcttca tctccgccat gcggaagtga
    13741 atgacctggt tgtcgatgag cggctgaccg aacgtgtggc gctctttgca atacgcgatc
    13801 gtcgcgtcga tgagcccttc catgccgcgc agcgtgccga cggcaccgca gaggcgctct
    13861 tcctggaatt gcaccatctg catcatgaag cccatgccct cttcaccgat gcgattgcgc
    13921 tgcggcaccc gcacgttctc gaagaacacg gggcacgtgt cgctcgcgcg catgccgagc
    13981 ttgtcgattt tcgcaccgat ctcgacgccc ttcgtcttcg ctgggataac gacgagcgtc
    14041 ttgttcatgt ggggcgcgcc ctcactcgtg cagacgagcg tgcagagcca gtccgcctgc
    14101 gcggcgttcg tgatccacat cttgctgccg ttgatcacgt agtcggcgcc atctttgcgc
    14161 gcggtcgttt tgatctgtgc gacatcggag ccacccgacg cttcgctcac tgcgatcgat
    14221 gcgatctgct cgcccgcgat cacgggcgcg aggaactcct gcttgagctc gtgagtgccc
    14281 caccgcgaga gcgccggtgt ggccatatcg gtcaacaccc cgagcgccat tggtatggag
    14341 cctgacgcac aagtcccgag ctcttcggtc acaaccattt gatacgaata gtcgagcccc
    14401 ataccgccgt aggcttcggg cttgttcaca ccgaggaggc cgaggcgacc ggctttcttt
    14461 gccacttcgt gcgctgggaa cgcgccatcg cgttcccact tatcgacatg cgggttgatt
    14521 tccttctcga cgaagtcacg caccgtgcgg cgcagctgct ggtgttcatc cgtgaagcgc
    14581 atcgggctga ccgtacagga agtgtgagaa ggtgtcatct gcacgaaagt tgtccggttt
    14641 tcgtacagag accggttcac ttgtgcagct gtttttcctt tgatcgcagg cgcgtgatca
    14701 tgggagtttg tactcgttcg ggagtaactt tactgaggtc tacgtgctgc cgatcattct
    14761 gaaaattcgc ctcatgggaa tggctgcagc gagctggact gcggccgctt caccgatgcc
    14821 gttgccggcg gaatcggtcg ctacgctatt cgcgacgcgc gctgcgacgc cggtcgacgt
    14881 cgatctctac gacgagaacg atcatcagaa gggccacatc gcgatctggc gcgatggatc
    14941 cgtcgacgaa gacacgcgcg tgatcgtcaa gcggatgttc cgctgccgca agacgcaccg
    15001 cgaagcgatg atccatcaca cgacgctcgc gatgctcgcc gatgtcgccg agcgctacga
    15061 aggcaagacg atcgagtacg tctcggcgtt ccgcgtcggc catgcagaat cgcgcacgtc
    15121 gccgcatcgt gatgcgcgcg cgatcgactt ccgcatccgc ggcaccaacc tgcgcgagat
    15181 tcgcgactac ctgtggcgta cgtacaccga agtcggcgtc ggctggtatc cgaagggcca
    15241 gtacattcac atcgacgcgc ggccgacgat gacggacatg tcatggacgt tcgtcaacgg
    15301 tacaaatcgg tatcatccgt actgggctga gctcgcgcgc aaaccggagc tcgagacgga
    15361 cgccgcgcag atggcgcacg ctcgtcgctc cggctcgtag tagcctcgcc gcgtgatttc
    15421 gcggttcgca ccgtcgacga cgggcgaggc tcatcccggc acgctgctgt cgggcttgct
    15481 cgtctggctc gacgcgcgca cgcgcaaagg ccaggcgctg ctgcgactcg aagatctcga
    15541 tcgcacgcgc gtgaaggaag cgtggtcgga tcagctcctc gaggcgacgc gctggctcgg
    15601 cctcgactgg gacgcggtga tcgtgcagag cgtccgcaac gcatcgcacg agcgcgcact
    15661 cgatcagctc gaggcgctgg gccgcctcta tccatgcacg tgcagtcgcg ccgatagggc
    15721 cggtggccgt cgcgcgcccg atggtagctg ggcgtacgag aacacgtgcc gcggcaagct
    15781 gctgcccgat ggcggctggc gtgcagtcga tggcagcgtg cgcgtgcagc tgcctgacga
    15841 acgcatcgag cttgtcgacg acggtggcct cgatctctcg caaacacccg cacacgatat
    15901 cggcgatccg atcgtgcgcc gtcgcgatgg cgtgatcgcg tatcagctcg cggtcgtcat
    15961 cgacgacgcg gacgaaggca tcaccgatgt cgtccgcggt cgcgacatcg cgccgagcac
    16021 cgcgacccag gtgatgctgc aacgactgct cggcctgccg acgccgcgct atcgccatca
    16081 cttcctgtta ctcgaacaca gtggagacaa gctcgcgaag ctgcacggct cgattccgtg
    16141 gagcgtcgtg cgcgcgcatt acagcggcga cgcgatgtgc ggcgcactcg cgcatgcggc
    16201 cggtctgctc gccacgccgc gcccgtgtac gccgagtgag ctcgtgcaca cgttcacgtg
    16261 gtcacgcgtg ccgaccgccg atcggctcac gcactggcgc gacaacaagc tcgtgttcga
    16321 gtgattactc gggcacgacg ccgttgatcg gcacccagat cgtttcgccg gagccgccga
    16381 cctcgagctc gtagtagccc tgcagcagct ggcgcaccgt cgccgattgc atcagcccgc
    16441 ccggtccggg cacgagcacg cgaccgccgg gtgcgatctt cttcgagccg ccgatctcgc
    16501 cggaggtaga tgcgctcgac gcaccgcccg agccgatcgc agacatgccg atgcccatga
    16561 tcgcgccgcg gtgcccctcg acgggcgtga tcttcgcgtc gttcgcggtc gacttcggcg
    16621 cctcgctgcc gggatccgtc ttgtcgtcgg ccgcctgcac ttcgaccgcg cccgcgcgac
    16681 gtggcgacga gggtggcacc ggcgtctgga tgacgcgatg tgggtcgggg gccggcgacg
    16741 ccgaagcccc agccgacgtg atcggtgccg ccgcggccgc atgtcccggc gccgtctgct
    16801 tctcctcgcg gagtggcttc tccttcggct tgaccacgat ctcgcccgac gcctcgacct
    16861 gcggatgcgt ctgcgcacgc ggcttcggcg tgcttgccgg cggtttcgcg gcgggcggcg
    16921 gcacttggcg gacggtctcg agcaggttcg atggctcggg acgcggcgca cgcgcagtct
    16981 cgaccagatg ctgctgatcg cggcgcgtat cgccgtttgc gggcagccgc gggttcgagc
    17041 cttgcgcggt catctgctgc cacggccgcg agccgtcgtc ggcgatgatc gacaggtggc
    17101 ccatgcgtac gagctcgaca ccgaacactg cgccggccgc cgggttgtag gcgaccagct
    17161 cttcgatgat gttgccgagc atctgtggat cggtgaccgc gttcggcgtt cccgccatca
    17221 cgacgcgacg tgtgagcaag cgcgcgagca tgcgctcgat gctgcgcgac acgctgcgca
    17281 ggacgccgtc gttcacggca tcgaacggca ggccgacgaa ctgcgcgacg agcagcgccg
    17341 gatccatgca acgaacttgt agtgaaccgt ttgcgcggat gcgcacgtgc tgtcccgtcg
    17401 ttggcagctg gaacagcgtg atcatgtcgt acgaaacttc gaccggcgcg gtgagcacga
    17461 agtacgccga cacggggcgc atcggatccg gtgtgcgcca gcgatgtcgt cctggtccga
    17521 gtcgatcgcc gaccatgaac gacgggcaca tcacgacgag ctcgtcgtcc gcgcacgaga
    17581 cctcgtactc ctccgggccg attggaaacg gccaggcgaa gactagatgg tcagacgcga
    17641 cagcgtcgcg cgcgatcacc ggcatcaccc gcaatactaa caggtttgcg acgagggaag
    17701 gtggcggcgg agtgcgccgt agaatgctgc gacgagttgg gacagtcgtc cggcggacgc
    17761 ctattcaccg gcgtcttcga ggccctcgtc catgtctgtc tcggccaccg gatggatgcg
    17821 cactctgcgc acgcggcgtg gcgatgcctc gatgacctcg aggaccgtgc cgttatcggt
    17881 agtcagcttt tcgccggcct gcgggattgc ctgcgcgagc gacatgcaca gtccggcaat
    17941 ggtcgtgcgg tccttaccga ccggcaggtc caggtggagg tcacggttga ctttgcgcac
    18001 cgccgcccaa ccttgcacga ggatcgtgcc ttgcggctcg cgcacgaagt actgctcgac
    18061 gacgtcgtcc tcgctcatga tgtcgccgac cagctcttcg atcaggtctt ccgcggtgac
    18121 gagacccgag aggccgccgt tgtcatcgac cacgaccgcc atctgggtcc ggcggcgctg
    18181 cagctcgcga agcagatcga gcgcgcgcgt cgactccggc accttgtgcg gcggccgcaa
    18241 gatgtcttcg agcacgacaa gacccttctc ccacgcgagt gcgagaacgt cgcgcgcgac
    18301 cacgtagccg acgatattgt cgatcgactg atcgaacacc ggcatgcggc tgtggccttc
    18361 ttcgaggatc acgcgctgca cttcttccgg cggcgcgccc cggcgcagcg cgacgacgcg
    18421 attgcgcggc accatcacct cggcgaccgt gacttcgccg aagccgagcg cacgcgacgc
    18481 gatctcgctc gcccgcggat cgaccgagcc ggtcttcgcg gcttcctcga cgagttgctg
    18541 cagctcgtcg cgcgacagcc gggtctcggt gaacgtggtc ttgtcgccga agaagcgcag
    18601 caagatgttg gacgcgctgg tgagcgtcca cacgaccggc cgtaacagct gcgagatgaa
    18661 cgtgagcggt cggccggcga agaacgagta gcgatcggag tagcggagcg cgagcgactt
    18721 cggcaccagc tcgccgatga cgagctcgag gtagacgatc atgatgacga cgagcacgag
    18781 cgccgtgcca tggccgaggc cgaactgcgt gagcgcgtgc gcgatggtcg gctccagctc
    18841 ggcaccgccg tacacggatg ccgccgtgcc gaacgtggtg atgcagacct gcacggtcgc
    18901 gaggaaccgc tcgggctggt cgcgcagctt cttgaccgcg agtgcgcgct tgtccttgcg
    18961 gcgaatgaac tcgcgcaggc gcgtcttgcg caccgacaga atggcgattt cggcgcccgc
    19021 gaagagcccg ttgccgacca gaaaggccag cacgatcagg atcgccgtac ccatgcgtgc
    19081 cggcgcgcat cttacgctat atgctgtcgc cgatgtcgac gcgggatcgc ccgcagccgc
    19141 cgccgaagaa gagctacatc acgcctgatg gagcgaagaa gcttcgcgcc gagctggacc
    19201 aattgtggac ggtcgagcgg ccgcgcgtga cgcaggaagt cgccgacgcc gcagcacaag
    19261 gtgatcgcag cgagaacgcc gagtacatct acggcaagcg ccgcctgcgc gagatcgatc
    19321 ggcgcgtgcg ctttttgagc aaacggctcg acgcggtcac gatcgtgagc gagcccccga
    19381 ccgatccctc gcgcgcgttt ttcggcgcgt atgtcacgct cgaagacgac gatggcaacg
    19441 aggtcaccta ccgcatcgtc gggcccgacg agtcggacgt cgacaaaggc tggatcagca
    19501 tggaagcgcc cgtcgcgaag gcgctgtttg gcaaacgcga aggtgatgac gtgacggtgc
    19561 gccgcccgaa aggtgacatc acgtatacga tcgtctccgt ccgctacgac acctgacgca
    19621 tggccgctcc tgctcctcgc cagcccgatg ccgtccccga accggtcgac gacaagccgt
    19681 cacgcgcgat cgcgatccag ttgccggcga cgatgcaggt ctacgcacgg tcgccgctgc
    19741 gctggcgagt cggctgcgac gctaccgtgc tccgcgacgg tgcggccacg tatcacgcga
    19801 tgctcgaagc gattcagggc gcaaagcagt cgatctgcct cgagacgtac atcctcgcgt
    19861 cggatacgac gggcgaccgc ttcaagctcg cgcttgtcga gcgggcgcgc gccggcgtca
    19921 agattcgcct catctacgac gcggtcggct cgttcggcat ctccgatgcg tgggtcgatg
    19981 atctgcgcac cgccggcatc gaggtcatcg acttcaaccc gatctcgccg tggcgtcgcc
    20041 gcttccggct ctcgcatcgc gatcaccgca agctgctgat cgtcgacaac gagatcggct
    20101 tcaccggtgg cctcaacatc gcgaacgact acgcgtccgt cgacgaaggc ggcgtcggct
    20161 ggcacgacat gcactgccgc gtgaaggggc cgatcgtcta cgacatggca cggctgtttc
    20221 gccgcaactg gatccgctcc ggaggcgacg catatccacc cgtaccgcgt cccgacgaag
    20281 gcagcggtca gggcgcgagc ttcatccgac tgctcgagaa cacgaagcgc cgcaacaaga
    20341 ccacgtttcg tcgtgcgtac ctccacgtca ttcgcgccgc gcgcagcttg gtgcggatcg
    20401 agaacgcata ctttttgccc gatcgcggcg tgcgtcgtgc gctcgttcgc gcggcgatgc
    20461 gtggcgtcga cgtccagatc atcgtccccg gtcactccga cattcgcctc atcgaatggg
    20521 cgagtctgta cgtgctgaag aatctcaccc gccgcggcat caaggtctgg cgctggcgcg
    20581 gcgtgatgat gcacgcgaag accgcgaccg tcgacgcgat ctggtcgacg atcgggagct
    20641 acaacttcga ctcgcagagt cgcttcaaca atctcgaagt caccgtcgag atcctcgatc
    20701 cggatgtcgg caacacgctc gtcaaagagt tcgacgccga catgccgaac tgcgacgcct
    20761 acgacgagac tgtgtggcgg caacttccat ggtggaagaa agcactcg
//
LOCUS       DASUFX010000422        14164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1318162, whole genome shotgun sequence.
ACCESSION   DASUFX010000422 DASUFX010000000
VERSION     DASUFX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318162"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFV99_16920"
     CDS             <1..937
                     /locus_tag="VFV99_16920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5061054.1"
                     /translation="APYLAPAHAYVFSRADYVPAAYLDPARATVIAPTIDPFSPKNQR
                     LDGRSMLAILSRSGLVASSVDLACATFNREDGTPGRVDRAADVLRDGPLPSETTPLIV
                     QVSRWDRLKDPIGVLEGFARLDETSARGAHLVLAGPNTSGVSDDPEGIAVLANVEAQW
                     RLLPDAVRRRVHLASLPTFDVDENAAIVNALQRQATIITQKSLHEGFGLTVTEAMWKA
                     RPVIASAVGGIQDQLDNGVEGILLPDATDLDAFAAAIARLLGNPDEARRMGEHGHARV
                     VEKCLGINSLLRYGALIELLDDGAAVRRHEAHAAP"
     gene            complement(945..1340)
                     /locus_tag="VFV99_16925"
     CDS             complement(945..1340)
                     /locus_tag="VFV99_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061055.1"
                     /translation="MKLDKSCALFFGTKIDSKLREGLALSKPGDKKYFDGSSEEFLRV
                     IEIGDDKDKERWIGKVIKAGPAVTEVDDIQRNILSILRRVAPNARIPASAVKIFVLQG
                     AASAAQVIDDEPEGDEAEEAGSSGPYITY"
     gene            complement(1352..1422)
                     /locus_tag="VFV99_16930"
     tRNA            complement(1352..1422)
                     /locus_tag="VFV99_16930"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1388..1390),aa:Gln,seq:ttg)
     gene            1530..1606
                     /locus_tag="VFV99_16935"
     tRNA            1530..1606
                     /locus_tag="VFV99_16935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1564..1566,aa:Met,seq:cat)
     gene            1689..3104
                     /locus_tag="VFV99_16940"
     CDS             1689..3104
                     /locus_tag="VFV99_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061056.1"
                     /translation="MWRFGVLALVGCDAVFALPHIREQPDVDAADTRGVTGRYGVLVI
                     EPDDFGGYLPPREQTVPTPFEVVLDDGDRPAITSDENFQFAFSRNRDGQSYRLRYTPY
                     GTSTPAVAITAARDLELFSTLFGRYEREPITKPTLLTVTFPQMQQTAYAFSTGLRTSA
                     QINETPTPLSSVAIDWMTQPPLSGLRGLPSATEHDRFYLAEYVLIGGVGAYYAIEKIA
                     SAPLTLADGAPATLNMTPALAPLGRCVRLTAGRSAEQDRLRAALPPASVEAMWSIEAT
                     PDPMVSPRGGLLLAYEDTTTTSDFQEDVRYPNPFAGERQVVRMLTKATYDLSGHTVTA
                     YTEHFAPLPVTDVCDAASAVTIAGSVAIPNAVTIDDQPAQGSVAVGTGRIAFALGGGG
                     SADYFAVEVLDAQTFATTVRYLNTEPTVTVDPGSLQPGSTYLLRLLAIQGAPGARTGD
                     FRPIAFPYGIAVTYAGPFSVQ"
     gene            3210..4259
                     /locus_tag="VFV99_16945"
     CDS             3210..4259
                     /locus_tag="VFV99_16945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5061057.1"
                     /translation="MDFALDDDQRMIVDTVRRFADRDLRAWSADADRNGAPPDRLTHV
                     AGELGFFVDAVPAAHDGLLEGAFSHATRALRGFELGRGCAALAALLEANVEPALAVAR
                     WGSDAAKKELFASLASGGVAALAHDSRATLEVIDGRVTGKLGPVPALARASHLLVVAK
                     DLVFVAPTAALKITPVTPSGWRAAQWATAQLDSFKPEHVLPAESAEHILAWARVSLAA
                     RAAGVATAAMEYAEAYAKERVQFGQPIGKFESLIRLRDDAETGAVAARTLALYAAWAL
                     DTNQPRALDLASRARVLAGDVVARATIDAVQIFGGYGFVNDYPVEKLMRDARAFEALH
                     GDERLGRVLAQKVVA"
     gene            4261..5490
                     /locus_tag="VFV99_16950"
     CDS             4261..5490
                     /locus_tag="VFV99_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5061058.1"
                     /translation="MDLFQDNPLFQGVRQMLGAFAKQAMRPIAIKHDQEESMPWELMK
                     SSQALGLTQTAVVDGRKKLTGVDEETDPKKPKSQARLAVVGSEELAYGCAGITLAIGG
                     SGLAATPVARMGTEEQKQEFRRLLSGVDEKGHIKVAAMALSEPATGSDISGLKTVARQ
                     DGDYWVIRGSKQWITNGQSAGVYVVWAQTDQSAGRAGVRGFIVGRDTPGLVPGKKETK
                     LGIRASETAQVHFEDCRVHKDMMLGVAASAASGGESAGGLADTKKMLDSTRPSVGAQG
                     VGIARAAYDFLLERVQSSSLHGTPMASHQHIMFELAEMAMEINASRLLVHRAAWLADH
                     GKDNVAEASMAKAHGARTAQYVTTRAMVLLGEEALEPGHPVEKWYRDAKIYDIFEGTG
                     QIQRRIIAKHLTGQNPT"
     gene            5632..7248
                     /locus_tag="VFV99_16955"
     CDS             5632..7248
                     /locus_tag="VFV99_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5061059.1"
                     /translation="MAIALGIDLGTTNSCAAVATPTGVEFILGQRAERVHPSVVAFPQ
                     TGGVVVGADAKRYRLTEPHHVIHSAKRFIGQNIRAPLVQLALTGIRYKVTEGPNQQPI
                     VVVRDRRMTMPEISSHVLLYLKRCAERQLGESVNDAVITVPANFTDAQRQATKEAGRL
                     AGLNVMRLINEPTAAALAYGFGRNQDQLICVFDFGGGTFDVSILRINGEVFEVLASDG
                     DYFLGGDDLDRALAEVLAAECHRTLHFDPRQHPVLMMKLMMGAEAIKCHLSENDAAEG
                     EIDGIDLPNGTTGSLPFALTRKQFEELISGYVNRTIEVTKQVVAQAGLLPEDISDVLC
                     VGGSTRIPTVRQRLADTFHREPNIKINPDEVVAQGAAIQAGSLSGSIISGTGMGARDA
                     VMTEAHSPLIHGGAVAKGPLARPVLLDVNPSTLSIQTAGGYAERLLDKNSPIPIERTR
                     VFTTARDNQTRVEIDCCRGESRRYTENEPLGQLLLEGLPPKSRGDLKIEVCFRVDADG
                     ILHVKASDAESGARQEVRMQVFGAPTGESP"
     gene            7245..7961
                     /locus_tag="VFV99_16960"
     CDS             7245..7961
                     /locus_tag="VFV99_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061060.1"
                     /translation="MSARTDVLLWANRVIARKGSAAHVLLEVTEQATPQQVQDAFHKI
                     ARTSHPDLHRHGLSNDELELVTSAYAAVAGAYQEMRMLQSARVRAALAKPPSDPPTPR
                     SGASTPTGGARTVHPRAGTDSGLPPIRQRAGTNSDAPRGRAGTDSDAPMRPGNVHATG
                     SSPPGTFTPEEPAAKPHDPAHAMSSKALLYYRKAEVCKKKGDLRGAMLQLKLACATDP
                     TSSFLRTALADVETELRSKP"
     gene            8026..9153
                     /locus_tag="VFV99_16965"
     CDS             8026..9153
                     /locus_tag="VFV99_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5061061.1"
                     /translation="MTTGTAISGHGVWHPANVLTNDELVAAFNEFVRRDNAKHAEEIA
                     AGTRQALKESSSEFIVKASGIVNRYVEDKTGLMDPDRMCPNIPDRPEEALSVQAEYSV
                     NAAKVALARAGRTGEDIDLVVLGASNLQRLYPAIAIEVQEAIGARGWALDMSLGCSAA
                     TGATIVASQAVQAGTARTALVVVPELTTGHMNWRDRDSHFIFGDASVALVVEKSENVK
                     AQGAWEILSTRMLSKYSATAIRNNMGYLDRCDPSTQFSDSKLFHQQGRRVFKDVVPLA
                     SKFILEHLEAHSVAPAQVSRYWLHQANHKLNSLVAERVLGREATREESPIILDEFGNT
                     ASAGSLIAFSRHSEDLGTGSYGLMCSFGAGYSLGSLMLQRR"
     gene            9158..9931
                     /locus_tag="VFV99_16970"
     CDS             9158..9931
                     /locus_tag="VFV99_16970"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX5061062.1"
                     /translation="MFGWLTERRRKRLLETPFPAAWTEIIERNVAAYKLLTADEQQRL
                     RDLVQVFIAEKHWEGAGGLELTDEIRVTIAGTGCQLLLGRDHDLFNEVASIVVYPSTV
                     RLPDAPRSFFDPGARPVGGDTAVLGVAHRGGAVVLAWDNALRGARDPRDGHNVVIHEL
                     AHKIDFLDGAADGTPPLPSGHARRAWIAAFAPAYLAHKERAERGERSFLDDYAVTNEA
                     EYFAVATEAFFEKPHALARELPDVYAALRQYYSLDLAER"
     gene            9947..11359
                     /locus_tag="VFV99_16975"
     CDS             9947..11359
                     /locus_tag="VFV99_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061063.1"
                     /translation="MSSEIFRDPTEGVNARRADLLRRRRDELVMMPHAIRRVYVARRA
                     RTAASVAIAFVGTALLAVAAKPAWAAYLAKGLPGINPAVLCTGIVAMWLVGLFTYFAA
                     RAVDEHRFAVAMSKLVMPGKDVNADVERLSHENPDQAARDMAHRLEVRSSAWPVIAAA
                     VLVPVTALYAAAIYRAGGWPVIAEFEASVAVHAKALLACAGVGTLIAFAMTKRTMRLP
                     IVGPMMMGLAIATSAGALFSLWLVPIALILATVALVVRRLRIERDLLQAEDPAAGSEI
                     WTIRGFIRQLRQAGGAAIARVRKIRRRRLVIGGVAAVAAVAGALVLVPAKPARVTTQV
                     KVATANQKVVTATVVTEPTGSKSMVEIHAMNDGRVGLRITLDIADDLPVSVPSLAGMA
                     NVPPMWNATVRITQIEGLGLVVSPFGGSEDMKNISVGEAITVTHANCGMGESPFGFRA
                     QGAPGHYVLFVEPTLAPVGC"
     gene            complement(11356..11910)
                     /locus_tag="VFV99_16980"
     CDS             complement(11356..11910)
                     /locus_tag="VFV99_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061064.1"
                     /translation="MASVTLFLQHIGLALRLASTHPQLEPAEVLAHAHAATVAATPQI
                     SAELLLGMAYVESRFDPLAVSRIEGKHRRVGHYPSTNPPVKLRKGSSLYCGPLQTFAG
                     TWDECMAARNLDVAYATAAKEIGRWLRDRRVRGDMTRALAGYGCGNRGVKTGQCNRYP
                     GRVLWQAKRLEIAKPISIMLAARS"
     gene            12114..13997
                     /locus_tag="VFV99_16985"
     CDS             12114..13997
                     /locus_tag="VFV99_16985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX5061065.1"
                     /translation="MFSETSAHEGAGQVILERALERFGHASLRPGQAEVIADIFGGTP
                     VIAVMPTGAGKSLCYQLPAVVLGERGGVTLVVSPLIALMKDQVDALRARGIPAVALTS
                     ASGADEQREILDGIRAGAFTLVYVAPERFRSPRFVEALFATAGRVALVAIDEAHCISE
                     WGHDFRPDYRRLGQVVAELKPPRLAAFTATATPEVRKDIAHQLGIEDARLHVHGFDRP
                     NLTYVVQKAGGTADKTDQLIELVRMREGGTALVYAATRKNAEAYSQALRKAGMRSRVY
                     HAGLEDEVREKAQDVFMAGQLDVIVATNAFGMGVDKSDIRLVVHADIPRSPEAYYQEA
                     GRGGRDGKPTRCVLLFNHGDIRLQEFLIDASFPSAELLRGLWKLLKEQPQLGTLTRNE
                     DELEARLKKHIDVPSGAALSSAIRILERHGMLARDDERLAAARPAPGQFMPLDVESLK
                     RRADIERKKLRTMIDYAYHPRCRRQFVLEYFGDADWGDRSKRCGACDNCEAVAHGKTT
                     GLSEAEADAVRSLLMLIGSLNGRFGRTRIAALANGSDDDPRFDEMPERGSLRGWSPQQ
                     IMDLVRALEGAGLVESSRGEYPTVSTTRRGDLVGIGKLDPNELGLQMPTVTKRSRVRK
                     RKR"
BASE COUNT         2410 a         4796 c         4657 g         2301 t
ORIGIN      
        1 cgcgccgtac ctcgcgccgg ctcatgccta cgtgttctcg cgcgccgact acgtgccagc
       61 ggcctatctc gatccagctc gcgcgaccgt gatcgcgccg acgatcgatc cgttctcgcc
      121 aaaaaaccaa cggctcgacg gccgctccat gctcgcgatc ttgtcgcgct ctggactcgt
      181 cgcgagcagc gtcgacctcg catgtgcgac gttcaaccgc gaagacggca cgcccggccg
      241 cgtcgatcgt gccgccgacg tgctgcgcga tggcccgttg ccgtccgaga cgacgccgct
      301 catcgtccag gtctcgcgct gggatcggct caaggatccg atcggcgtgc tcgagggttt
      361 cgcgcgtctc gacgagacaa gcgcccgcgg tgcgcacctc gtgctcgcgg gcccgaacac
      421 cagtggcgtg agcgacgatc ccgaaggtat cgccgtgctc gcaaatgtcg aggcgcaatg
      481 gcgcctcttg cccgatgccg tgcgccggcg cgttcacctc gcgtcgctac cgacgttcga
      541 cgtcgacgag aacgccgcga tcgtcaatgc gctgcagcgc caggcgacga tcatcacgca
      601 gaagagtctg cacgaaggct tcgggctgac cgtgaccgaa gcgatgtgga aagcacgccc
      661 cgtgatcgcg agcgcggtcg gcggcatcca agatcagctc gacaacggcg tcgagggcat
      721 cctgctcccc gatgcaaccg acctcgacgc gtttgctgca gcgatcgcgc ggctgctcgg
      781 caaccccgac gaggcgcgcc gcatgggcga gcacggccac gcgcgggtcg tcgagaagtg
      841 cctcgggatc aactcgctcc tgcgctatgg cgccctgatc gagctactcg acgacggcgc
      901 cgccgtcagg cgccacgaag cccacgcggc gccgtaacac gtgcctagta cgtaatgtac
      961 ggcccgctcg atccggcctc ttccgcctcg tcgccttcgg gctcgtcatc gatgacttga
     1021 gcagccgacg ccgcgccctg cagcacgaag atcttcacgg cagacgcagg gattcgagcg
     1081 ttgggcgcca cgcggcgcag gatcgagagg atgttgcgct ggatgtcgtc gacctcggtg
     1141 accgcggggc cggccttgat gaccttgccg atccagcgtt ccttgtcctt gtcgtcgcca
     1201 atctcgatga cccgcaggaa ctcttccgag ctaccgtcga agtacttctt gtcgccaggc
     1261 ttggacagcg cgaggccttc gcgcagcttg ctgtcgatct tggtgccaaa gaagagagcg
     1321 cagctcttat cgagtttcat gatcactttc gttcggggac aaggattcga accttgataa
     1381 gaagctccaa aggctcctgt cctaccatta gacgatcccc gatcacggcc gcaatctagc
     1441 tgcaaaaccc tgactcgcca acccatcctc ctgaattcgt tgggagcttg acagcttccg
     1501 ctgaacccac tagggtctgc gccgaatttg gctgggtagc tcagctggtt agagcagggg
     1561 tttcataagc ccaaggtcgg cggttcaagt ccgccctcag ccaccaagtt ctagcgcggc
     1621 gtacgcgatc attcgcagct ctccacccgg aaccggtcgg tgagctcgac cacgggtatc
     1681 attggtcggt gtggcgcttc ggggtgctgg cgctggtcgg ctgtgacgct gtgttcgcgc
     1741 tgccccacat ccgtgagcag ccggacgtcg atgcagcgga cacgcgcggg gtgaccgggc
     1801 gctacggcgt gctcgtgatc gaaccggatg acttcggcgg gtacctaccg cctcgcgagc
     1861 agacggtgcc tacgccgttc gaggtcgtgc tggatgacgg tgaccggcct gccatcacca
     1921 gcgacgaaaa ctttcagttc gcgttctcgc gcaatcgcga cggtcagtcg tatcgcttgc
     1981 gctacacgcc gtacggtacc tcgacgccag ccgtggcgat caccgccgcg cgtgacctcg
     2041 agctcttctc gacactgttt ggacgctacg aacgcgagcc gattacgaag ccaacgctgc
     2101 tgacggtgac gtttccgcag atgcagcaga cggcgtacgc gttctcgacg ggcctgcgca
     2161 cgagtgcgca gatcaacgag acaccaacgc cgctcagcag cgtcgcgatc gattggatga
     2221 cgcagccacc gctgtcaggt ctgcgtggct tgccgtcggc caccgaacac gatcggttct
     2281 acctcgccga gtacgtactg atcggtggtg tcggggctta ctacgcgatc gagaagatcg
     2341 cgtcggcgcc gctgaccttg gccgacggcg cgcccgcgac gctgaacatg acgcccgcgc
     2401 tcgcgccact cggacgctgc gtgcggctga ctgcggggcg ttccgccgag caagatcgtc
     2461 tacgcgccgc gctgccacca gcgagcgtcg aagcgatgtg gtcgatcgaa gcaacgccgg
     2521 acccgatggt gagtccgcga ggtggcctgc tgctcgcgta tgaagacacg acgacgacgt
     2581 ccgacttcca ggaggatgtt cgctatccga atccgttcgc gggcgagcgg caagtggtgc
     2641 gcatgctcac gaaggcgacg tatgacctta gcggccacac ggtgacggcg tacaccgagc
     2701 actttgcacc gctgcccgtc accgatgtct gcgatgcggc gagcgcggtc acgatcgctg
     2761 gcagcgtcgc gattccgaac gcggtgacga tcgatgacca gccggcacaa ggcagcgtgg
     2821 ccgtcggaac cgggcgcatt gcgttcgcgc tcggcggcgg cggctcggcg gattacttcg
     2881 ccgtcgaggt gctcgatgcg caaacgttcg cgacgactgt gcgctacctc aacaccgagc
     2941 cgacggtgac cgtcgatccg gggtcgctcc agccaggttc gacgtacctg ctccgcctcc
     3001 tcgcgatcca gggcgctccc ggcgccagga caggcgactt ccggccgatc gcctttccct
     3061 acgggatcgc ggtcacgtac gcgggcccgt tcagcgtcca gtagcctcgc tgggccgcag
     3121 cccggctatg tgctggtacc cccgaccgcc gacgtaacaa tttacagtct ctgctgactg
     3181 tttccgccag acgcggtaca gctcgttgca tggacttcgc actcgatgac gatcagcgaa
     3241 tgatcgtcga taccgtgcgc cggttcgccg atcgcgacct gcgtgcttgg tccgccgatg
     3301 ctgatcgcaa cggtgcccca cccgatcgcc tgacgcatgt cgcgggcgag ctcgggttct
     3361 tcgtcgatgc ggtcccggcc gcgcacgacg gcctgctcga aggcgcgttc tcacacgcga
     3421 cccgcgcact gcgtggcttc gagctcggtc gcggctgcgc tgcacttgcg gcgctgctcg
     3481 aggcgaacgt cgagcccgcg ctcgccgttg cacggtgggg cagcgacgcc gcgaagaagg
     3541 agctgttcgc atcgctcgca tcgggcggtg ttgcggcgct cgcgcacgac agccgcgcca
     3601 cgctcgaggt gatcgacggc cgcgtcaccg gcaagctcgg cccggtccct gccctcgcac
     3661 gcgcgagtca tctgctcgtc gtcgcgaagg acctcgtgtt cgtcgcgccg accgcagcgc
     3721 tgaagatcac gccggtcaca ccgtcgggtt ggcgcgccgc gcagtgggcg accgcgcaac
     3781 tcgatagctt caaacccgag cacgtgctgc cggcggaaag cgccgagcac atcctcgcgt
     3841 gggcgcgcgt gtcgcttgcg gcccgcgctg ccggtgtcgc gactgccgcg atggaatacg
     3901 ccgaggcgta cgcgaaggaa cgcgtgcagt tcggtcagcc gatcggcaag ttcgaatcgc
     3961 tgatccgctt gcgcgacgac gccgagaccg gtgccgttgc cgcacgcacg ctcgcgctgt
     4021 atgcggcatg ggcgctcgac acgaaccagc cgcgcgcact cgatcttgcc agccgcgccc
     4081 gcgtgctcgc cggtgatgtc gtcgcgcgcg cgacgatcga tgccgtgcag atcttcggcg
     4141 gctacggctt cgtcaacgat tatcccgtcg agaaattgat gcgcgacgcc cgcgcattcg
     4201 aagcactcca cggcgacgag cgactcggtc gcgtcctcgc gcagaaagtg gtggcctgac
     4261 atggatctct ttcaagacaa tccactgttc caaggcgtgc gccagatgct tggcgcgttt
     4321 gcgaagcagg cgatgcgccc gatcgcgatc aagcacgacc aagaagagtc gatgccgtgg
     4381 gagctgatga agtcgtcgca agctctcggc ctcacgcaga ccgccgtcgt cgatggccgc
     4441 aagaagctga ccggcgtcga cgaggaaacc gatccgaaga agccgaagag ccaagcgcgc
     4501 ctcgccgtcg tcggtagcga agagctcgcg tacggctgcg ccggcatcac actcgcgatc
     4561 ggtggctccg gtctcgccgc gacgcccgtc gcgcgcatgg gcaccgagga gcagaagcag
     4621 gagttccgcc ggctgctctc gggcgtcgac gagaaaggcc acatcaaggt cgccgcgatg
     4681 gcgctctccg agcccgcgac cggctcggac atctcgggcc tcaagacggt cgcgcgccag
     4741 gacggcgact actgggtgat tcgcggcagc aagcagtgga tcacgaacgg ccagtccgct
     4801 ggcgtctacg tcgtgtgggc gcagaccgat cagtccgcag gccgcgccgg tgtgcgcggg
     4861 ttcatcgtcg ggcgcgacac gccgggcctc gtgcccggta agaaggagac gaagctcggc
     4921 atccgcgcgt ccgagaccgc gcaggtccac ttcgaggact gccgcgtcca caaggacatg
     4981 atgctcggcg tcgctgccag cgcagccagc ggcggcgaat ccgcgggtgg ccttgccgac
     5041 accaagaaga tgctcgattc gacgcggcca tcggtcggcg cgcagggcgt cggcatcgcc
     5101 cgcgccgcgt acgacttctt gctcgagcgc gtgcaaagca gctcgctgca cggcacgccg
     5161 atggcgagcc accagcacat catgttcgag ctcgccgaga tggcgatgga gatcaacgcg
     5221 tcgcggttgc tcgtgcatcg cgccgcctgg cttgccgatc acggcaagga caacgtcgcg
     5281 gaagcgtcga tggcgaaggc gcacggcgcg cgcaccgcgc agtacgtgac gacccgcgcg
     5341 atggtgctcc tcggcgaaga ggcgctcgag cccggtcacc ccgtcgagaa gtggtaccgc
     5401 gacgcaaaga tctacgacat cttcgagggc accggtcaga tccagcgccg catcatcgcg
     5461 aagcacctga ccggccaaaa cccgacctag cgacccaacc tgctacaccg cgccgcgcta
     5521 cacctctata agaggacttc tttccaggca gcgatggggt agcgacggga ttcgtgttgt
     5581 cgaagacccg aggtgcgacc tccagttggg ttatttttgg tcgagaacgg gatggcgatt
     5641 gcactcggca tcgacctggg gaccacgaac tcgtgcgcgg cggtggcgac accgaccggc
     5701 gtcgagttca tcctcggcca gcgcgccgag cgcgtgcatc cgtccgtggt cgcgtttccg
     5761 cagaccggcg gtgtcgttgt cggcgctgac gcgaagcggt atcggctgac cgagccgcat
     5821 cacgtcatcc actcggcgaa gcggttcatc ggtcagaaca tccgcgcgcc gctcgtccag
     5881 ctcgcgctca ccggcatccg ctacaaggtc accgaagggc cgaaccagca gcccatcgtt
     5941 gtcgtgcgcg atcgccggat gacgatgccg gaaatcagct cgcacgtgct gctctacctg
     6001 aagcggtgcg cggagcgtca gctcggcgag tcggtcaacg acgccgtgat caccgtgccg
     6061 gcgaacttca ccgacgcaca gcggcaagcg acgaaggaag ccggccggct cgccggcctc
     6121 aacgtgatgc gcctgatcaa cgagccgacc gcggccgcgc tcgcatatgg cttcggccgc
     6181 aatcaggacc agctgatctg cgtgttcgac ttcggcggcg gcacgttcga cgtcagcatc
     6241 ctgcgcatca acggcgaggt gttcgaggtg ctcgcctcgg atggcgacta cttcctcggc
     6301 ggcgacgatc tcgatcgcgc actcgcggaa gtgctcgctg ccgagtgcca tcgcacgctc
     6361 cacttcgatc cgcgccagca cccggtgctg atgatgaagc tcatgatggg cgcggaggcg
     6421 atcaagtgtc acctctccga gaacgacgct gcggagggtg agatcgacgg catcgatctg
     6481 ccgaacggca ccaccggctc gctgccattc gcgctcacgc gcaagcagtt cgaagagctg
     6541 atctcgggct acgtcaaccg cacgatcgaa gtcacgaagc aggtcgtcgc acaggcaggt
     6601 cttctacccg aggacatcag cgacgtgctc tgcgtcggcg gttcgacgcg aattccgacc
     6661 gtgcgccagc gcctcgccga cacgttccat cgcgagccga acatcaagat caatcccgac
     6721 gaggtcgtcg cgcaaggcgc cgcgatccag gcgggcagcc tctcgggctc gatcatctcg
     6781 ggcacgggca tgggcgcgcg cgacgcggtg atgacggaag cgcactcgcc actgatccac
     6841 ggcggcgcgg tggcaaaggg gccgctggcc cgcccggtgc tactcgacgt caatccatcg
     6901 acgctgtcga tccagaccgc gggtggctac gccgagcgct tgctcgacaa gaactcgccg
     6961 attccgatcg agcgcacgcg cgtgttcacg accgcacgcg acaatcagac gcgcgtcgag
     7021 atcgattgct gtcgcggtga atcgcggcgc tacaccgaga acgagccgct cggtcagctg
     7081 ttgctcgaag gtctgccgcc gaagtcccgc ggcgatctga agatcgaagt gtgcttccgc
     7141 gtcgacgccg acggcatcct gcacgtcaaa gcgagcgatg ccgaatcggg cgcgcgccag
     7201 gaagtgcgca tgcaggtgtt cggcgcgccg acgggagaat ccccgtgagc gcccgcaccg
     7261 acgtcctgtt gtgggccaat cgcgtgatcg cgcgcaaggg aagtgcggcg cacgtgctgc
     7321 tcgaggtcac cgaacaggcg acgccgcagc aagtgcagga cgccttccac aagatcgcgc
     7381 gcacgtcgca tcccgatctg catcgccatg gtctcagcaa cgacgagctc gagctcgtca
     7441 ccagtgcata cgcggcagtc gcaggcgcgt atcaggagat gcgcatgctg cagagcgcgc
     7501 gcgtgcgagc agcccttgcc aaaccgccgt cggatccacc gacgccacgg agcggtgcgt
     7561 cgacgccgac cggcggtgcg cgaaccgtgc atccacgcgc cggcaccgat agcggcctgc
     7621 cgccgatccg acagcgcgcg ggtaccaact cggatgcacc gcgtggtcgg gccggtacgg
     7681 attcggatgc accgatgcgg cctggaaacg tgcacgccac cggttcgtct ccgcccggga
     7741 cgttcacgcc cgaagagccg gcagcaaagc ctcacgatcc cgcgcatgcg atgtcgtcca
     7801 aagcgctcct ctattaccgc aaggccgagg tctgcaagaa gaagggcgac ctcaggggcg
     7861 cgatgttgca gctcaaactt gcgtgcgcca ctgatccgac ctcttcgttc ctacgaacgg
     7921 cactcgccga cgtcgagacg gaactacgca gtaaaccgta gatcttctga ccgggtggac
     7981 agagccccga tcctgtgacc aactccccgc gcaatgccgc cgcggatgac gactggtaca
     8041 gcgatttcag gtcacggagt ctggcacccc gcaaacgtcc tcaccaacga cgagctcgtc
     8101 gctgcgttca acgagttcgt gcgtcgcgac aacgcgaagc acgcagagga aatcgcagct
     8161 ggtacgcgtc aggcgctgaa ggagagctcc tccgaattca tcgtcaaggc gtcgggcatc
     8221 gtcaaccgct acgtcgaaga caagaccggg ctcatggatc cggatcggat gtgtccgaac
     8281 attccggacc gtcccgagga ggcgctcagc gttcaggccg agtactccgt caatgcggcg
     8341 aaagtagcgc tcgcgcgtgc gggccgcacc ggcgaggaca tcgatctcgt cgtgctcggc
     8401 gcgtcgaacc tgcagcgcct gtatccggcg atcgcgatcg aagtccagga agcgatcggc
     8461 gcgcgcgggt gggcgctcga catgtcgctc ggctgttcag ccgcgaccgg cgcgacgatc
     8521 gtcgcgtcgc aagcggtcca ggccggcacc gcgcgcaccg cgctcgtcgt cgtgcccgag
     8581 ctgacgaccg gccacatgaa ctggcgcgat cgcgacagtc acttcatctt cggcgatgcg
     8641 tcggtggcgc tcgtcgtcga gaagagcgag aacgtcaaag cgcagggcgc gtgggagatc
     8701 ctgtcgacgc gcatgctgtc gaagtactcg gcgaccgcga tccgcaacaa catgggctac
     8761 ctcgatcgct gcgatccgag cactcagttc tcggacagca agctgttcca tcagcagggt
     8821 cgccgcgtgt tcaaggatgt cgtgccgctc gcatcgaagt tcatcctcga gcatctggaa
     8881 gcgcacagcg tcgcgccggc gcaggtcagc cgctactggc tgcaccaggc gaaccacaag
     8941 ctcaacagcc tcgtcgccga gcgagtactc ggccgcgaag cgacgcgcga agagtcgccg
     9001 atcatcctcg acgagttcgg caacaccgcg tcggcgggct cgctgatcgc gttctcgcgc
     9061 cacagcgaag acctcggcac cggcagctac ggcctcatgt gctccttcgg cgccggctac
     9121 tcgctcggct cgctgatgtt gcagcgccgc tagccgcatg ttcggctggc tcaccgagcg
     9181 tcgtcgcaag cgtctgctcg agacgccgtt tccggccgca tggacggaaa tcatcgagcg
     9241 caatgtcgcg gcgtacaagc tgctgaccgc cgacgagcaa cagcggctgc gcgacctcgt
     9301 gcaggtgttc attgccgaga agcactggga gggcgccggt ggcctggagc tcactgacga
     9361 gatcagggtc acgatcgccg ggaccggctg ccagctgcta ctcggccgcg accacgatct
     9421 gttcaacgag gtcgcctcga tcgtcgtcta tcccagcacg gtccggttgc ccgacgcgcc
     9481 gcgcagcttc ttcgatccgg gcgctcgccc ggtcggcggc gacaccgccg tgctcggcgt
     9541 cgctcaccgc ggcggcgcag tcgtcctcgc gtgggacaac gcactacgcg gagcgcgcga
     9601 tccccgtgac ggccacaacg tcgtgatcca cgagcttgcc cacaagatcg acttcctcga
     9661 cggggccgcc gatggcacgc cgccccttcc ctccggacac gcgcgccggg cctggattgc
     9721 agcgttcgct cctgcctacc tcgcccacaa ggagcgcgcc gagcgcggcg agcgcagctt
     9781 tctcgacgat tacgcggtga caaacgaagc ggagtacttc gccgtcgcca ccgaggcgtt
     9841 cttcgaaaaa ccacatgcgc tcgcccgcga gctgcccgat gtgtacgccg cgctgcgcca
     9901 gtattattcc ctagatctcg cagaacgttg agctagcgtt tacggggtga gcagcgaaat
     9961 cttccgcgac cccaccgaag gcgttaacgc acgaagagcc gacctgctgc gtcgccggcg
    10021 cgacgagctc gtcatgatgc cgcacgcgat ccgtcgcgtg tacgtcgcgc gtcgagcacg
    10081 cacggcggcc agcgtcgcga tcgcgttcgt cggcactgcc ttgctcgcag tcgctgcgaa
    10141 gcccgcgtgg gccgcgtacc tcgcgaaggg cttgccgggc atcaatccag cggtgctgtg
    10201 caccggcatc gtcgcgatgt ggctcgtcgg tctgttcacg tacttcgcgg cgcgcgccgt
    10261 cgacgagcat cgcttcgcgg tcgcgatgtc gaagctcgtg atgccgggca aggacgtcaa
    10321 cgcagacgtc gagcggctct cgcacgagaa tcctgatcaa gcagcgcgcg acatggcgca
    10381 tcgcctcgag gtgcgatcgt cggcgtggcc agtgatcgcg gctgcggtgc tcgtgccggt
    10441 gaccgcgctc tacgccgctg cgatctatcg cgcgggtggc tggccggtga tcgccgagtt
    10501 cgaagcatcg gtcgccgtgc acgcgaaggc gctgctcgct tgtgctggtg tcggcacgct
    10561 gatcgcgttc gcgatgacga agcgcacgat gcgcctgccg atcgtcgggc cgatgatgat
    10621 gggcctcgcg atcgcaacca gcgcgggcgc gctgttctcg ctgtggctcg tgccgatcgc
    10681 gctgatcctc gcgaccgtcg cgcttgtcgt ccgtcggctc cggatcgagc gcgacctgct
    10741 gcaggccgaa gacccggcag cgggcagcga gatctggacg attcgcggct tcattcgcca
    10801 gctccgccaa gcgggcggtg cggcaatcgc acgcgtcagg aagatccgcc gccgtcgtct
    10861 cgtgatcggt ggtgttgctg ccgtcgcggc ggtcgccggc gcgctcgtgc tcgtaccggc
    10921 gaagccggcg cgggtcacca cgcaggtgaa ggtcgcaacc gcgaaccaga aggtcgttac
    10981 cgcgacggtc gtcacggagc cgacgggcag caagtcgatg gtcgagatcc atgcgatgaa
    11041 cgacggcaga gtcggcctgc gcatcacgct cgacatcgcc gacgacctgc cggtctcggt
    11101 gccgtcgctc gcgggcatgg cgaacgtgcc gccgatgtgg aacgcgaccg tgcgtatcac
    11161 gcagatcgag ggcctcggcc tcgtggtgtc gccgttcggc ggcagcgagg acatgaagaa
    11221 catctcggtc ggcgaggcca tcaccgtcac gcacgcgaac tgcggcatgg gcgagtcgcc
    11281 gttcggcttc cgcgcgcagg gcgctcccgg ccactacgtg ctgtttgtcg agccgacgct
    11341 ggcgcccgtg ggctgctagc tacgtgccgc gagcatgatc gagatgggct ttgcgatctc
    11401 gagtcgcttt gcctgccaca gcacgcgacc gggatagcgg ttgcactggc cggtcttcac
    11461 gccgcgattg ccgcagccat agccggcgag cgcgcgggtc atgtcgccgc gcacgcgcct
    11521 gtcgcgcaac cagcggccga tttccttcgc ggccgtcgcg tacgccacgt cgaggttgcg
    11581 tgcggccatg cattcgtccc aggtgccagc gaatgtctgg agcggcccgc agtacagcga
    11641 cgagccttta cgtagtttca cgggtggatt cgtcgacggg taatggccga cgcggcggtg
    11701 cttgccttcg atgcgcgaca ccgcgagcgg atcaaaccgg ctctcgacgt acgccatccc
    11761 cagcagaagc tcggcggaga tttgcggcgt ggccgcaacg gttgcagcgt gtgcgtgggc
    11821 gaggacctct gccggctcga gctgcggatg cgtcgatgcc aatcgcaacg cgagtccgat
    11881 gtgctggagg aagagggtca cactcgccat cgccaacgcc ctaggagcaa ctacgaggct
    11941 agcccacgat ctgtggacgg gctgtgaaga agcggtggaa tggcagtgaa ttcgcgaatc
    12001 cggagcgggc gatttcgtca atgccgcgca atctgcgccg gattcgcgaa aggtccgacc
    12061 cacctcgtcg acgattcacg aaaaggtcgg acccagctga tacggtgcgc tggatgttca
    12121 gcgagaccag cgcacacgag ggcgcgggcc aagtgattct cgagcgcgcg ctcgagcgct
    12181 tcggccatgc gtcgttgcgc cctggccaag ccgaggtcat cgccgacatc ttcggcggca
    12241 caccggtgat cgcggtgatg ccgaccggcg cgggcaagtc gctctgctat cagctgcccg
    12301 cggtcgtgct cggcgagcga ggcggcgtca cgctcgtcgt ctcgccgctg atcgcactca
    12361 tgaaggatca ggtcgacgcg ctgcgcgcac gcggtattcc cgccgtggcg ctcacctcgg
    12421 cgtcgggtgc cgacgagcaa cgcgaaatcc tcgacggcat tcgcgcaggc gcgttcacgc
    12481 tcgtctacgt cgcgcccgag cggtttcgca gcccgcgctt cgtcgaggcg ctgttcgcga
    12541 ccgccggccg cgtcgcgctc gtcgcgatcg acgaggcgca ctgcatctcg gaatggggtc
    12601 acgacttccg gcccgactac cggcggctcg gtcaggtcgt cgccgagctc aagccaccgc
    12661 gcctcgccgc attcaccgca accgcaacac ccgaggtgcg caaggacatc gcgcaccagc
    12721 tcggcatcga ggacgcgcgc ctgcacgtcc acgggttcga tcggccgaac ctgacctacg
    12781 tcgtgcagaa ggcgggcggc accgccgaca agacggatca gctcatcgag ctcgtccgca
    12841 tgcgcgaagg cggcaccgcg ctcgtctacg cggcgacgcg caagaacgcc gaggcgtact
    12901 cgcaggcgct gcgcaaagcg ggcatgcgtt cgcgcgtcta tcacgccggc ctcgaggacg
    12961 aggtccgcga gaaggcgcaa gacgtgttca tggcgggtca gctcgacgtg atcgtcgcga
    13021 cgaatgcgtt cggcatgggc gtcgacaaga gcgatatccg gctcgtcgtc cacgccgaca
    13081 ttccgcgcag ccccgaggcg tactaccagg aagcaggacg cggtggccgc gacggcaagc
    13141 cgacgcggtg cgtgctcctc ttcaaccacg gcgacattcg cctgcaagag ttcttgatcg
    13201 acgcctcgtt cccgtcggcg gagctgttgc gcggcctgtg gaagctgctc aaggagcagc
    13261 cgcagctcgg cacgctcacg cgcaacgagg acgagctcga agcgcggctc aagaagcaca
    13321 tcgacgtgcc gtccggtgct gcgctgtcgt cggcgattcg catcctcgag cgccacggca
    13381 tgctcgcgcg cgacgacgaa cggctcgccg ccgcacgtcc cgcgcccggt cagttcatgc
    13441 cgctcgatgt cgagtcgctg aagcgccgcg ccgatatcga gcgcaagaag ctgcgcacga
    13501 tgatcgacta cgcgtatcac ccgcggtgcc ggcggcagtt cgtgctcgag tatttcggcg
    13561 acgccgactg gggcgatcgc agcaagcgct gcggtgcatg cgacaactgc gaagcggtcg
    13621 cgcacggcaa gacgaccggc ctctcggaag ccgaggccga tgcggtgcgc agcctgctca
    13681 tgctgatcgg ttcgctcaac ggccgcttcg gtcgcacgcg catcgcggcg ctcgcgaacg
    13741 gcagcgacga cgatccgcgc ttcgacgaga tgcccgagcg cggcagcctg cgcggttggt
    13801 cgccgcagca gatcatggat ctcgtccgtg cactcgaagg cgcagggctc gtcgagtcat
    13861 cgcgcggcga gtacccgacg gtctcgacca cacggcgcgg cgacctcgtc ggtatcggca
    13921 agctcgatcc gaacgagctc ggcttgcaga tgccgacggt gacgaagcgc tcgcgcgtcc
    13981 gcaaacgaaa gcggtagtgc gcgagcacgc gagcgtccgg tcgatgctcg gtggccggac
    14041 acgcgcgtcc gcatcgcggc caaaccggac acgcgtcgtc gagaattcgc gcgcttgcgc
    14101 tggcaccgta catgctcgcg tcccgtgcat gacctcgact gctatagtcc gccgcatgac
    14161 gatg
//
LOCUS       DASUFX010000423         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1318361, whole genome shotgun sequence.
ACCESSION   DASUFX010000423 DASUFX010000000
VERSION     DASUFX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318361"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFV99_16990"
     CDS             <1..666
                     /locus_tag="VFV99_16990"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5061066.1"
                     /translation="DNDLEATDYTVGFQTAVEIVVEALERLHTTAESHERVMVVEVMG
                     RYAGWIALTAGLAGGAHVVLIPEIDYDPEKVLAAFHRRHGRGVSYSIVVVAEGAKPKG
                     GTLAVEKAGDATKQEKLGGAGQRLTELIASRTEYEVRNTVLGHVQRGGSPCAFDRELG
                     TRFGVKAVELIAEGKFGHVAAIESGRLVAKPIGTAVKKLKLVDPTGELVRAAKGIGIE
                     LGG"
     gene            672..1736
                     /locus_tag="VFV99_16995"
     CDS             672..1736
                     /locus_tag="VFV99_16995"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HEX5061067.1"
                     /translation="MKPAVVPAPRSVIDRLVERRATEPHIGAWDQPLDNPNIEDAQIA
                     HALDRFAGMPAIGRVEQLYRQKLRLKSWQYMTAVTDDLFIAFVVGTAGFASNGFVYAA
                     ELPSGRVHKRFAITPLMKGTKLAHSSTAAAHLFHARNLSVAIENRGRAFSAHIDARTE
                     AKDKLRAQLEFDSEPADDHLAVCVPLPDGRWNYTHKFAAFSVTGHVTIGDRRIDFARD
                     RSFGTMDFSKMFALRHAVWRWIALSGKTDSGKRVGINLVDPTPGAPISENALWLDGKR
                     HALTNVQVAQDLTSARADGLELEMSDVAEVAQRLDLPLVRHRLRHVVGAFRAKFSTSS
                     GARENITRAVGIAEDYDTWW"
     gene            1833..3629
                     /locus_tag="VFV99_17000"
     CDS             1833..3629
                     /locus_tag="VFV99_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061068.1"
                     /translation="MYGERRFTGCGLTLLGVQRIGRYEILSHLASGGMGQVYLARSTG
                     LGGFERQVVVKTLDLSITDDDEAFVTMFLDEARLVGALHHQYIAPVYEVGCDEEGRYY
                     LVMDYVHGETAELVYRASAERAWPVPWAFALTVVSSVASALDYAHNLCAADGTPLDIV
                     HRDISLSNVMVGYDGGVKLIDFGIAKAANRATKTQVGTLKGKIGYLAPEQILRKNVDH
                     RADIFSLGIVLYELTTATRAFRDSSDLVTLERITSGDVKAPSSFTPNYPPELERIVMK
                     ALQVDPDQRYQDAAQLGRELERLAAQMNLPLGHAAIASSMKHLFGDMRRGRRRFARGS
                     DVKTDPSIQIIEDDEEKTPVVDVSSIQPDPQARARAARPTTPIAKRSGHESIDADADD
                     LPTPVSGERALPVGPSLRPTPPGDRRLITPLPDLSRSTAQVAAMSGAEMAVPTTRRAT
                     PTQRMPSPPFGERQALPLPASNAPPPRVAQHTPTGQHAVPAVRLPTGPQLPVVVFHAQ
                     GSEIAELPTHITGMRTDHEATDFPVPPRPPPPPLSAAHAPLVRARWAPSTFTHAVVAR
                     VKPVASKATRTWFILCGLFVLAVATAIAIAML"
     gene            complement(3626..4426)
                     /locus_tag="VFV99_17005"
     CDS             complement(3626..4426)
                     /locus_tag="VFV99_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061069.1"
                     /translation="MTYRDDLAALTARKEALDAEVAERTKERDEAARMLAEATARLRL
                     PVLDNIRVASPCKAKWADMIGDDRVRHCGKCDKDVFNLSAMTREDAEQLLREKNGDLC
                     ARYFRRADGTIMTQDCPVGQRTRRRVVAVVAGTMATLTGVAAYRGLVVEHTMGAVAKP
                     PEHEAVQGGVSMDPDPPPVVLASTGMPAECEQYRTEINLIQQCQQMPHDASLALANAW
                     QQFATAVKDIPPESMAPVRDACSAARDALIQARETMCAPPAGEQPIKR"
     gene            complement(4486..>6013)
                     /locus_tag="VFV99_17010"
     CDS             complement(4486..>6013)
                     /locus_tag="VFV99_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061070.1"
                     /translation="FEPFSASSRRVGGTALGLSISRTIIEAHGGRIWVESGSGGTKFV
                     FTLPVSAAKEKTVQLPTALEAQPRRRGLGENSCALVVDDDPRRALLLKGLLMSLAERV
                     LVCHDVDSALATARAEHPAIAVVAGAMTDALALLAILEHDPDTHKTAVMVVGDSERRA
                     DMLAAGADDLLEFPIQPAMFRDACLRLVESGRREAPRVLIVDDDPSIRTICREVLELG
                     GYQVRDAGSATAALGEARRFRPDMILLDVLMPGIDGYRTAEMIRADPAIGMAPIMFLS
                     ARGDTADKVRAFRSGAEDYMVKPFDAAELLARVAKALDRQARELGASPTTQLPGADAI
                     QAEIERRLAACDAGAVACYLDLDNLKAFNDYYGYAKANAVIRQTGDVIRYVVQRSGGP
                     GDFIGHIAGDDFVFITSADRADDVCRAICERFDHLIRLYYDPADRERGYIETKDRFGV
                     QRKFPIMSVSIAAISLTRAKSYAGLAELAAVGKRTAKAIPGSSYVRDGQTMAAETATG
                     "
BASE COUNT         1012 a         1976 c         2026 g          999 t
ORIGIN      
        1 gacaacgacc tcgaggccac cgactacacg gttggcttcc agactgcggt cgagatcgtc
       61 gtcgaggcgc tcgagcggct gcacacgacc gcggagagcc acgagcgcgt gatggtcgtc
      121 gaggtgatgg gccgttacgc gggctggatc gcgctgaccg ccgggcttgc cggcggcgca
      181 cacgtggtgc tgattccgga aatcgactac gaccccgaga aggtgctcgc agcgtttcac
      241 cgccggcacg gccgcggcgt cagctattcg atcgttgtcg tcgccgaggg cgcgaagccg
      301 aagggtggca cgctcgcggt cgagaaggcc ggcgacgcca cgaagcaaga gaagctcggt
      361 ggggcagggc agcggctcac cgagctgatt gcatcgcgca ccgagtacga agttcgcaac
      421 acggtgctcg gccacgtcca gcgtggcggc tcaccgtgcg cgttcgatcg cgagctcggg
      481 acgcggttcg gcgtcaaagc ggtcgagctg atcgccgagg gcaagttcgg tcacgtcgcg
      541 gcgatcgagt cgggtcgcct cgtcgccaag ccgatcggga ccgcggtcaa gaagctcaag
      601 cttgtcgatc cgacgggcga gctggtgcgg gcggcgaagg gcatcggcat cgagctcgga
      661 ggttagtcgt ggtgaaacct gctgtcgtac ccgcgccacg ctccgtgatc gatcgcctcg
      721 tcgagcgccg cgcgaccgag ccgcacattg gcgcgtggga tcaaccgctc gacaatccca
      781 acatcgaaga cgcgcaaatc gcgcacgcgc tcgatcgctt cgccggcatg cccgcgatcg
      841 gccgcgtcga acagctgtat cgccagaagc tgcgactcaa gtcgtggcag tacatgaccg
      901 cggtcaccga tgacctgttc atcgcgttcg tggtcggtac tgccggcttc gcgagtaacg
      961 gcttcgtgta cgccgctgag ctgccgagtg gccgcgtgca caaacgcttc gcgatcacac
     1021 cgctgatgaa aggcacgaag ctcgcgcact cgagcactgc agccgcgcac ttgtttcacg
     1081 cgcggaatct ttcggtcgcg atcgagaatc gcgggcgagc attctcggcg cacatcgacg
     1141 cgcgcaccga ggcgaaagac aagctgcgcg cgcagctcga gttcgatagc gagcctgctg
     1201 acgatcatct cgccgtatgc gtgccgcttc ccgatggtcg ctggaactac acgcacaagt
     1261 tcgccgcgtt ctcggtgacc ggtcacgtca cgatcggcga tcgccgcatc gacttcgcgc
     1321 gcgatcgatc gttcggcacg atggacttct cgaagatgtt cgcgctgcgt cacgcggtgt
     1381 ggcgatggat cgcgctctcc ggcaagacgg atagcggcaa gcgcgtcggt atcaacctcg
     1441 tcgatccgac gcctggtgca ccgatcagcg agaacgcgct gtggctcgac ggaaaacgtc
     1501 atgcgctgac gaatgtccaa gttgcgcaag acctcacgag cgcgcgtgcc gacgggctcg
     1561 agctcgagat gagcgacgtc gccgaagttg cgcagcgtct cgatctgccg ctcgtacgtc
     1621 accgcttacg ccatgtcgtt ggcgcgtttc gcgcgaagtt ctcgacgagc agcggcgcgc
     1681 gcgagaacat cacgcgggcg gttggtatcg cagaagacta cgacacctgg tggtgaggac
     1741 tcgtgcacca ccgtgcacac gaatgtccgc acaacattgg ttggacctgt tcggttcact
     1801 aaagtattgc ttgcgagatc gcgtgtgcgt gtgtgtatgg tgagcgccgt ttcacagggt
     1861 gtggactgac tttgctcggc gttcaacgga tcggtaggta cgaaatcctg agccatctcg
     1921 cctccggcgg catggggcag gtgtacctgg ctcgttcgac cggcctcggc ggtttcgagc
     1981 gccaggtcgt cgtcaagacg ctcgacctca gcatcaccga cgacgacgaa gcgttcgtga
     2041 cgatgttcct ggacgaggcg cgcctggtcg gtgcgctcca tcatcaatat atcgccccgg
     2101 tctacgaggt cggttgcgac gaagagggtc gctactacct cgtgatggac tacgtgcacg
     2161 gcgagaccgc cgagctcgta tatcgcgcga gcgcggaacg cgcgtggccg gtgccgtggg
     2221 cgttcgcgct cacggtcgtg tcgagcgtcg catcggcgct cgactatgcg cacaaccttt
     2281 gcgctgcaga cggcacgccg ctcgacatcg ttcatcgcga catctcgcta tcgaacgtga
     2341 tggtcggcta cgacggcggc gtgaagctga tcgacttcgg catcgcgaaa gcggcgaatc
     2401 gcgccacgaa gacgcaggtc ggtacgctca aaggcaagat cggctatctc gcgcccgaac
     2461 agatcctgcg caagaacgtc gatcatcgcg cggacatctt ctcgctcggc atcgtgctct
     2521 acgagctgac gacggccacg cgcgcgttcc gcgatagctc ggatctcgtg accctcgagc
     2581 ggatcacgag cggcgacgtc aaggcgccgt cgtcgttcac gccgaactat ccacccgagc
     2641 tcgagcgcat cgtcatgaag gcgctgcagg tcgatccgga tcagcgctac caggacgcgg
     2701 cgcagctggg gcgcgagctc gagcggctag ccgcacagat gaacctgcca ctcggtcacg
     2761 cggcgatcgc ctcgtcgatg aagcacttgt tcggcgacat gcgacgcggt cgccgtcgct
     2821 ttgcgcgcgg cagcgacgtg aagaccgatc cgagcatcca gatcatcgag gatgacgagg
     2881 agaagacgcc ggtcgtcgac gtttcgtcga tccaacccga tccgcaggct cgcgcacgcg
     2941 cggcgcgccc gaccacgccg atcgcgaagc gcagcggcca cgagtcgatc gacgccgacg
     3001 ctgacgacct gccgacgccg gtcagcggcg agcgcgcact tccggtcggt ccgtcgctgc
     3061 ggccgacacc gccgggcgat cgccggttga tcacgccgct acccgatctg tcgcgtagca
     3121 ccgcgcaggt agcagcgatg tccggcgcag agatggcggt gccgaccacg cggcgtgcga
     3181 caccgacgca gcgaatgccg tcgccgccat tcggcgagcg gcaagcactg ccactgccgg
     3241 cgagcaacgc accgccgccg cgcgtcgcgc agcacacacc gacggggcag catgcagttc
     3301 cggcagtgcg gctaccgacc gggccacagc tacctgtcgt cgtgttccac gcgcagggca
     3361 gcgagattgc cgagttgccg acgcacatca cgggcatgcg caccgatcac gaggctaccg
     3421 acttcccggt gccgccgagg ccgccaccgc cgccgctttc ggcggctcac gcaccgctcg
     3481 tgcgcgcgcg ctgggcgcca tcgacgttca cgcatgccgt ggtcgcgcgc gtgaagccgg
     3541 tcgcgtcgaa ggcgacgcgg acatggttca tcctctgcgg cttgttcgtg ctcgcggttg
     3601 cgaccgcgat cgcgatcgca atgctctaac gcttgatcgg ctgttcgcct gcgggcggcg
     3661 cacacatcgt ctcgcgcgcc tggatgagcg cgtcacgtgc ggcgctgcat gcatcgcgca
     3721 ccggggccat cgactcgggc gggatgtcct tcacggcggt tgcgaactgc tgccaggcat
     3781 tcgcgagcgc gagcgatgcg tcgtgcggca tctgctggca ctgctggatc agattgatct
     3841 cggtgcggta ctgctcgcac tcggccggca ttccggtcga ggcgagcacc acaggcggcg
     3901 gatccggatc catcgaaacg ccaccttgaa ctgcctcgtg ctccggcggt ttggcgactg
     3961 cgcccatggt gtgctcgacg acgagcccgc gatacgccgc gacaccggtg agcgtcgcca
     4021 tcgtgcccgc gacaaccgcg accactcggc gccgcgtgcg ctgaccgacc ggacagtcct
     4081 gggtcataat cgtgccgtcg gcgcgccgga agtaacgcgc gcatagatcg ccgttcttct
     4141 cgcgcaacag ctgttccgcg tcttcgcgcg tcatcgccga caggttgaag acgtccttgt
     4201 cgcacttccc acagtggcgc acgcggtcat cgccgatcat gtccgcccac ttcgccttgc
     4261 acggcgatgc gacgcggatg ttgtcgagca ccgggaggcg aagtcgcgca gttgcttcgg
     4321 cgagcatccg cgcggcctcg tcgcgctcct tcgtgcgctc ggcaacctcg gcatcgaggg
     4381 cttctttgcg ggcggtcagc gcagcgaggt catcgcggta ggtcatggcg tctagtcaga
     4441 caccagccgc cgcggggttt gttgcagcga tctcgtaact gcccgtcagc ccgtcgcggt
     4501 ctcggcggcc atcgtctggc catcgcggac gtagctcgag cccgggatgg cctttgccgt
     4561 gcgcttaccg acggcggcga gctcggcaag accggcgtag gacttcgcgc gcgtcagcga
     4621 gatcgctgcg atcgacacgc tcatgatcgg aaacttacgc tggacgccga agcggtcctt
     4681 ggtctcgatg tagccacgtt cgcgatcggc tggatcgtag tagagccgga tcaagtgatc
     4741 gaagcgctcg cagatcgcgc ggcacacgtc gtcggcgcga tcggcgctcg tgatgaatac
     4801 gaagtcgtca ccggcgatgt gaccgatgaa gtcgccggga ccgccgctgc gttgcacgac
     4861 gtagcgaatc acgtcgccgg tctggcgaat caccgcgttc gctttcgcgt agccgtagta
     4921 gtcgttgaac gccttcaggt tgtcgaggtc gaggtagcag gcgaccgcgc ccgcatcgca
     4981 cgccgcgaga cggcgctcga tctcggcctg gatcgcgtct gcaccgggca actgtgtggt
     5041 cggcgatgcg ccgagctcgc gtgcctgacg atccagcgcc ttcgcgacgc gcgcgagaag
     5101 ctccgccgcg tcgaacggct tgaccatgta gtcttcggcg ccgctgcgga acgcgcggac
     5161 cttgtcggcg gtgtcaccgc gcgccgacag aaacatgatc ggcgccatgc cgatcgccgg
     5221 atcggcgcgg atcatctcgg cggtgcggta accgtcgatg ccgggcatca agacatcgag
     5281 caagatcatg tcggggcgga accggcgcgc ttcgccgagc gccgcggtcg ccgagccggc
     5341 atcgcgcacc tggtagccgc cgagctcgag gacttcgcga cagatcgtgc gaatcgacgg
     5401 gtcgtcatcg acgatcagca cgcgcggcgc ctcgcggcgg ccgctctcga cgaggcgtaa
     5461 gcacgcgtcg cggaacatcg cgggttggat cgggaactcg agcaagtcgt cggcgccggc
     5521 agcgagcatg tcggcgcggc gttcggagtc gccgacgacc atgaccgcag tcttgtgcgt
     5581 gtcggggtcg tgctcgagga tcgcgagcag ggcgagcgca tcggtcatcg cgccggcgac
     5641 gaccgcaatt gccggatgct cggcgcgcgc ggtagcaagt gcgctgtcga cgtcgtggca
     5701 gacgagcacg cgctcggcga gcgacatcaa caggcccttg agcagcaacg cgcggcgcgg
     5761 atcgtcgtcg acaacgagcg cgcacgagtt ctcgccgagc ccgcggcggc gcggttgcgc
     5821 ttcgagcgcg gtcggcagct gcaccgtctt ctccttcgcg gcagagaccg gcagcgtgaa
     5881 gacgaacttc gtaccgcccg agccagactc gacccagatg cggccgccat gcgcttcgat
     5941 gatcgtgcgc gagatcgaca gaccgagtgc ggtaccgccg acacggcggc tcgatgccga
     6001 gaacggctcg aag
//
LOCUS       DASUFX010000424         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_208383,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000424 DASUFX010000000
VERSION     DASUFX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208383"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFV99_17015"
     CDS             <1..353
                     /locus_tag="VFV99_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061071.1"
                     /translation="RETHEELGIDLTGARLLGNLPVLSPFTSGPMGVEVTPFVFVTHA
                     AVEPQPGPEADSAFWLPIELAASGALDDTYTYPGTSRTFPSWRFESHTIWGLTWRILG
                     DLLQAAKPDDVARP"
     gene            complement(315..1907)
                     /locus_tag="VFV99_17020"
     CDS             complement(315..1907)
                     /locus_tag="VFV99_17020"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEX5061072.1"
                     /translation="MVVSRAAAGVVYTPRDVCEPMVRLALAPLLGRDDLLSLRICDPA
                     IGEGAFLIEIVRVLAEALAARGIAPAKARTLIAQHCLFGVDIDPRAVATARTVVEDFV
                     GARVPALREHLRVGDALAIDWPHEFDALVGNPPYVRQELLANKPALRRFAAFDGVADL
                     YVYFIELAQRIARRYCLIVPSKWMTAAYGRPLRELLARTGSVSGIVDFARGLPLFSDA
                     DAFPCIVWGSRDDRGPLRAARITQPTSVADALAAPGIEHARDRWRADPWHIDDPDDRA
                     LIDRLAGRWPSLAQVVPERPSRGVVTGLNRAFVIDTVTRDALLADEPAAASLIRPFIK
                     GRDIRRWQPAPIDRYILLVDRGTSLAELPRVRAHLTQFRDALEPGSGRKPGSYKWFEL
                     QDPVVPLAKSRAPRLFYQDIQTAPACCLDPSGELVPDTTVWILPSSDPFLLAVLNSPL
                     YAWYARRRFPPALNGAVRPKLEYMRTLPIATPSATVRKKISALVTRQLADADPAIDRE
                     LAELVNAAYELGPRDVVRLGSL"
     gene            complement(1942..2628)
                     /locus_tag="VFV99_17025"
     CDS             complement(1942..2628)
                     /locus_tag="VFV99_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061073.1"
                     /translation="MKSLALLLAIACSGCFSTWAGMQAGGHSLAWDENKREETVPLPA
                     TDERLVLALPLHGDAALSCKSAQHARETVYRASFRYGSGWKKATALAFVTEAAIATAY
                     ALAGNRDDPRTYVYGGFFAIDAVGTAALFFVPRKDRFAKTERDVATPLREDCPEGLTI
                     EIAGTTYPVDAAGKLGEAGELALEDWLKAPTGELLASYAGRTTRLMVEPQQALGVLAV
                     PLGTLTALAE"
     gene            complement(2625..4418)
                     /locus_tag="VFV99_17030"
     CDS             complement(2625..4418)
                     /locus_tag="VFV99_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061074.1"
                     /translation="MKRFAYLLVLAACGGGHAHTTAPTTPTAPTTSTAATTTTTTTAA
                     TSIRLESFVAHQAWYRSPAACGQGPYEIVIPATELKWGERVELMLHTTHGVALRGVLL
                     VDGVEVEHINGVFDGEGSNAHLDNARCLADKRERVAAGRASGGGTGGTPVPVAVHVPG
                     TPSTPSTHAQLELEGEPVVGWTSLLRFRLNQPARSIVLRLWSVEPNDFEGVRIGTLKL
                     AWRPNVPEAEYEAYLVRLEVEARERAAREAAERERQAAAAQLREEQQREWLRQRDAKL
                     RAQREAELREIYACRSEAQRRARGEQVALGSVEHEQRCRQIAADDEVRRKRAIEAAEE
                     ARRHARENDEAAQRRAHEAEEEERRRARDAEEAARRREQEVRAAKLRLEIAAEKKRQH
                     ERFCASHPDNRGCWGAGGMTVHLQLDAAMREREQFCTTHHDDVRCWTTAEWTRHRTAW
                     NKRVELASAPAAQPEGPPPAPLAEDVPPKLSVHAEWRPGYWLWLDGKWVWLAGMWRVP
                     DADIVAEQTTTAPAAPPPVRVETPPPPPVVSAVWVTGFWQWNGTSWVWIAGSYQLRPS
                     AQVSWRAPEWRARGSVHVLIPGGWIRIGGSR"
BASE COUNT          719 a         1591 c         1564 g          764 t
ORIGIN      
        1 ttcgcgagac tcacgaggag ctcggcatcg atctcaccgg cgcgcgcttg ctcggcaact
       61 tgccggtgct gtcgccgttc acgtcgggcc cgatgggcgt ggaagtgacg ccgttcgtgt
      121 ttgtcacgca cgcagcggtc gagccgcagc cggggcccga agccgactct gcgttctggt
      181 tgccgatcga gctcgccgca tcgggcgcgc tcgacgacac gtacacgtat ccgggtacgt
      241 cgcgcacgtt cccgagctgg cgcttcgaga gccacacgat ctggggcctc acgtggcgaa
      301 tcctcggcga tctgttacag gctgccaagc cggacgacgt cgcgcggccc tagctcgtac
      361 gcagcgttga ccagctcggc gagctcgcga tcgatcgctg gatccgcatc ggcgagctga
      421 cgcgtgacga gcgcggagat cttcttgcgc accgtcgctg acggtgtggc gatcggcagc
      481 gtgcgcatgt actcgagctt cggtcgcacg gctccgttga gcgcgggtgg aaagcggcgg
      541 cgcgcatacc acgcgtaaag cggcgagttg agcactgcga gcaggaacgg atccgatgac
      601 ggcaggatcc acaccgttgt atcgggtacg agctcaccgc ttggatcgag acagcacgcg
      661 ggcgcagtct ggatgtcttg atagaacagc cgaggcgcgc gcgatttcgc gagcggcacg
      721 accggatctt gcagctcgaa ccacttgtac gacccgggct tgcgaccgct gccgggctcg
      781 agtgcatcgc ggaactgggt gaggtgcgcg cggacgcgcg ggagctcggc gagcgacgta
      841 ccgcgatcga cgagcaggat gtagcgatcg atgggcgcag gctgccagcg ccggatgtca
      901 cgccctttga tgaacgggcg gatcagtgag gcggcggcgg gctcgtcggc gagcagcgca
      961 tcacgtgtca cggtgtcgat gacgaatgcg cgattgaggc cggtgacgac gccacgcgat
     1021 gggcgctccg gcacgacctg ggcgagcgat ggccagcgac cggcgagacg atcgatgagt
     1081 gcgcggtcgt cgggatcgtc gatgtgccac ggatctgcgc gccagcgatc gcgcgcatgc
     1141 tcgatgccgg gcgcggcgag cgcatcggcc acgctcgtcg gttgcgtgat acgcgccgcg
     1201 cgaagtggac cccggtcgtc gcgagatccc cagacgatgc acgggaatgc atcggcgtcg
     1261 ctgaacagcg gcaagccgcg cgcgaagtcg acgatgccgg agacactgcc ggtgcgcgcg
     1321 agcagctcgc gcagtggacg accatacgcg gccgtcatcc acttgctcgg cacgatcagg
     1381 cagtagcggc gcgcgatgcg ttgcgcgagc tcgatgaagt agacgtagag atccgcgacg
     1441 ccgtcgaagg cggcaaacct gcggagcgcc ggcttgttcg cgagcagctc ctggcgcacg
     1501 tagggcggat tgccgacgag cgcgtcgaac tcgtggggcc agtcgatcgc gagcgcatcg
     1561 ccaacgcgca ggtgctcgcg cagcgccggc acgcgcgcgc cgacgaagtc ttcgacgacc
     1621 gttcgcgcgg tagcgacggc tcgcggatcg atgtcgacgc caaacaggca gtgctgcgcg
     1681 atcagcgtgc gcgctttcgc aggcgcgatg ccacgcgcgg ccaacgcctc ggcgagcacg
     1741 cgcacgatct cgatcaggaa cgcgccttcg ccgatcgccg gatcgcagat gcggagcgac
     1801 agcagatcat cgcggccgag cagcggcgcg agcgcgagcc gcaccatggg ttcgcagacg
     1861 tcacgcggcg tgtagacgac gccggctgcc gcacgcgaga cgaccatgcc tcgacgatac
     1921 ctggcagatc gatcgcgtca actactctgc gagcgccgtc agcgttccga gcgggaccgc
     1981 gagcacaccg agcgcctgct ggggctccac catcagcctg gtcgtcctgc cggcgtagct
     2041 cgctagcagc tcaccggtgg gtgccttcag ccaatcctcg agcgcgagct cgcctgcttc
     2101 gccgagcttg cccgcggcat cgaccggata cgtcgtgccc gcgatctcga tcgtgaggcc
     2161 ttcggggcag tcctcgcgga gcggcgtcgc gacgtcgcgc tcggtcttcg cgaagcggtc
     2221 cttgcgcggc acgaagaaca gcgcagccgt gccaactgcg tcgatcgcga agaagccacc
     2281 gtagacgtac gtgcgcgggt cgtcgcggtt gccggcgagt gcatatgcgg tcgcgatcgc
     2341 tgcctcggtc acgaacgcca gcgcagtcgc tttcttccag ccactgccgt aacggaacga
     2401 cgctcgatac acggtctcgc gtgcgtgctg cgccgacttg cacgacagcg cggcgtcacc
     2461 gtgcaacggt agcgcgagca cgagacgctc gtcggtcgcg ggtagcggca cggtctcctc
     2521 tcgtttgttc tcgtcccacg cgagcgagtg accgcccgcc tgcatgccgg cccaggtgct
     2581 gaagcaaccg ctgcacgcga ttgcgagtaa caaggcgaga gacttcatcg cgaacctccg
     2641 atccggatcc agccaccggg aatgagcacg tgcaccgaac cgcgcgcacg ccactcgggc
     2701 gcacgccagc tgacttgcgc cgacggacgc aattgatacg agcctgcgat ccacacccac
     2761 gacgtcccgt tccactgcca gaaccccgtg acccacaccg cgctcaccac cggtggaggc
     2821 ggcggcgttt cgacgcgcac cggcggcggc gcagcgggtg ccgtcgttgt ctgctcggcg
     2881 acgatgtcgg cgtcgggcac ccgccacatg ccggcgagcc agacccattt accgtcgagc
     2941 cacagccagt aaccgggccg ccactcggcg tgcacgctga gcttcggtgg cacgtcttcg
     3001 gcgagcggtg ccggtggtgg accctcgggt tgtgccgcgg gcgcgctcgc gagctcgacg
     3061 cgcttgttcc acgccgtgcg atgacgcgtc cactccgcgg tggtccagca gcgcacgtcg
     3121 tcgtgatgcg tcgtgcagaa ctgctcgcgc tcgcgcatgg cggcgtcgag ctgcaggtga
     3181 accgtcatgc cgccggcgcc ccagcagccg cgattgtcgg gatgcgacgc gcagaaccgc
     3241 tcgtgctgcc gcttcttctc ggctgcgatt tccagtcgca gctttgcggc acgcacttct
     3301 tgttctctgc gccgcgcagc ttcctctgcg tcacgagcac gccggcgctc ctcttcctcg
     3361 gcctcgtggg cacgccgctg cgccgcttca tcgttctcgc gcgcatgacg acgtgcttct
     3421 tctgccgctt cgatcgcacg ctttcggcgc acttcatcgt cggctgcgat ctggcggcag
     3481 cgctgttcgt gttccacgct gccgagcgct acttgctcgc cacgtgcacg gcgctgcgct
     3541 tcggaccggc acgcgtagat ttcgcgcagc tcggcttcgc gttgtgcgcg cagtttcgcg
     3601 tcacgctggc gcagccattc acgctgctgt tcttcacgga gctgtgcggc tgcggcctgg
     3661 cgctcgcgct cggcggcttc ccgcgcggca cgttcgcgcg cttcgacctc gagccgcacg
     3721 agatacgcct cgtactcggc ttcgggcacg ttcggtcgcc acgccagctt gagcgtcccg
     3781 attcgcacgc cctcgaagtc gttcggctcg accgaccaca accggagcac gatcgagcgc
     3841 gcgggttggt tcaagcgaaa cctcagcagc gacgtccacc cgaccacggg ctctccctcg
     3901 agctcgagct gggcatgcgt actcggcgtg ctcggcgtgc ccggcacatg caccgcgacc
     3961 ggtaccggcg ttccgcccgt cccaccaccg ctcgcgcgac ctgcggcgac acgctcgcgc
     4021 ttgtcggcga ggcatcgcgc gttatcgaga tgcgcattgc tgccctcgcc gtcgaagaca
     4081 ccgttgatgt gctcgacctc gacaccatcg acaagcaaga cgccgcgcaa cgcgacgcca
     4141 tgcgtggtgt gcagcatcag ctcgacccgc tctccccact tgagctcggt cgcggggatc
     4201 acgatctcgt acgggccctg gccgcacgca gccggcgagc ggtaccacgc ctgatgggcg
     4261 acgaacgact cgagacggat cgacgttgcc gccgtggtcg tcgttgttgt cgtcgcggcc
     4321 gtcgacgtcg tcggggctgt cggcgttgtc ggcgctgtcg tgtgcgcgtg accgccgccg
     4381 cacgccgcga gcaccagcag ataagcgaat cgcttcatgc acgaatagac gtggcactcg
     4441 acgaagatgg gaactttttg cgattgtgaa cagcggttta cgctgaacag cggttagatc
     4501 gggccatgcg ctggttttgt ttggtcgcga tcgccgcgtg ctgtcccccg atgagcgaca
     4561 agtcgacgac gcctgtcgtc gcaacgccac agcctccgcc gccgatcaaa cccgctgttg
     4621 cgcccgcgcc gccggcac
//
LOCUS       DASUFX010000425         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_902285,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000425 DASUFX010000000
VERSION     DASUFX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902285"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFV99_17035"
     CDS             complement(<1..536)
                     /locus_tag="VFV99_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061075.1"
                     /translation="MVKDDAPGTRADPAAPTVDGTKGTVSPHAPTEARADETIVPSWG
                     TSSSVALPDPGYQLGDMIGHGGMGEVLVAQDQRIGREVAVKRIRSREPSPDAVARFLR
                     EARIQARLDHPAIVPVYELGTDGDGRPYFTMKRLAGVTLAKRIASNAPVQPMLRAFVD
                     VCLAIQLAHTRGVVHRDL"
     gene            672..2243
                     /locus_tag="VFV99_17040"
     CDS             672..2243
                     /locus_tag="VFV99_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5061076.1"
                     /translation="MHGRRLVPWLVFLIPAVAHAEDAPVAPAAPYTPPDFLSATPPLP
                     TTIDATAVWRLDLDEALQLAVRQNLGVTLERKSVRISELGVTVANGLFEPTVAASYAH
                     GSSRTPPESSTEGMSGEILEFVNDAWRVSLGQRLATGMQLSVDFISDRAKSSRDTAVT
                     PISYRSTLEVSITQPLLRGFSLDLDVPRMTVLEAQLGSAKEKQQLETVVADVVQRTEA
                     AYWDVVQALFRYDLERRSHKRAEEQLALTKRQIESGTLPPSDMISAESTLAQRQLQLV
                     QAEEAIALAWDQLRAILNLPRDQWNRPILPVDVPHFAPNETSPDAAMTTAVSHRPELV
                     AMDIELRASALAVRKAENDKLPQIDLGLTGSVVGQDDRYRGALDQLSTFDSRGWSVLL
                     NLTWTPLRRATKAAAEIERTRHEVNVTRREQLVQEIWFAVRDAVRNQRSAARQVEAAA
                     RFRELAEKSLEVEQRKFLNGTSSNFLVAQRQEELAAAQLSELTAVLGHTKAAAALARA
                     TGQLLDDRHIQLGVK"
     gene            2326..2754
                     /locus_tag="VFV99_17045"
     CDS             2326..2754
                     /locus_tag="VFV99_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061077.1"
                     /translation="MTADLPIDAKPFDLWPLVPKSIQAIMPNVMWNRARLQRLDLPVR
                     DIPVAQLRWQLDLPWWRHGNKVFAITPNQVRNDPETFVIQFRRMLDADLDFPIHVLER
                     DRLILLDGFHRLLKADMLEMRTISARVVNLAQFADVLERR"
BASE COUNT          496 a          996 c          923 g          468 t
ORIGIN      
        1 ttgagatcgc ggtgcacgac gccgcgcgta tgtgcgagct ggatcgcgag gcagacgtcg
       61 acgaacgcgc ggagcatcgg ttgcacgggc gcattcgacg cgatgcgctt cgcgagcgtc
      121 acgccagcga ggcgtttcat cgtgaaatac ggccggccgt cgccgtcggt gccgagctcg
      181 tacaccggca cgatcgccgg gtgatcgaga cgggcctgga tgcgagcctc tcgcagaaag
      241 cgcgcgacag cgtcgggtga cggttcgcgc gagcggatgc gcttgacggc aacctcgcgg
      301 ccaatgcgct ggtcctgcgc gaccagcacc tcgcccatgc cgccgtgacc gatcatgtca
      361 ccgagctgat agccaggatc cggaagcgcg acgctcgacg acgtgcccca gctcggcacg
      421 atcgtctcgt cggcacgcgc ttcggtcggc gcgtgcggcg agacggtgcc cttcgtgccg
      481 tccacggtgg gcgcagccgg atcggcccgc gtccccggcg catcatcttt gaccatttgg
      541 ttcgatggta tccgattcgt tcgattgaca cgatggcgca accggcacgg ggcatccgtg
      601 tcagttctgc attggcgcga atgtcagacg aagcacctcg acagcgcgct cggcgcattt
      661 acgattcggc gatgcatggc cgtcgtctcg tcccatggct tgtttttctg atccccgcgg
      721 tcgcgcacgc ggaggacgcg ccggtagcgc cagccgcgcc atacacgccg cccgacttcc
      781 tgtcggcgac gccgccattg ccgacgacga tcgacgcgac ggcagtctgg cgcctcgatc
      841 tcgatgaagc gctgcagctc gcggtacgcc agaacctcgg cgtcacactc gagcgcaagt
      901 ccgtgcgcat ctccgagctc ggcgtcaccg tcgcgaacgg cctgttcgag ccgaccgtcg
      961 cagcgtcgta cgcgcacggc agctcgcgca cgccgccgga atcgtcgacc gaaggcatgt
     1021 cgggcgagat cctcgagttc gtcaacgatg catggcgcgt gtcgctcggc cagcggctcg
     1081 caaccggcat gcagctgtcg gtcgacttca tcagcgatcg tgcgaagtcg tcgcgcgaca
     1141 ccgccgtcac cccgatcagc tatcgctcga cgctcgaggt cagcatcacg cagccgctgt
     1201 tgcgcgggtt ctcactcgat ctcgacgtgc cgcggatgac cgtgctcgaa gcgcagctcg
     1261 gcagcgccaa ggagaagcag cagctcgaga cggtcgtcgc cgacgtcgtg cagcgcaccg
     1321 aggccgcgta ctgggacgtg gtccaggcat tgttccgcta cgacctcgag cgccgctcgc
     1381 acaagcgcgc cgaagaacag ctcgccctga cgaagcgcca gatcgagtcc ggcacgcttc
     1441 cgccgtccga catgatctcg gccgagagca cgctggccca gcgccagctt cagctcgtgc
     1501 aggccgaaga agcgatcgcg ctcgcgtggg atcagctccg cgccattctg aacctgccac
     1561 gcgatcagtg gaaccggccg atcctgccgg tcgacgtgcc gcacttcgcg ccgaacgaaa
     1621 cttcacccga tgcggcgatg acgaccgcgg tctcccaccg ccccgagctc gtggccatgg
     1681 atatcgagct gcgcgcgtcg gcgctcgcgg tcaggaaagc cgagaacgac aagcttccgc
     1741 agatcgatct cggcctcacg ggtagcgtgg tcggccagga cgatcgatac cgcggcgccc
     1801 tcgatcagct cagcacgttc gacagccggg gctggagcgt gctcctgaac ctcacctgga
     1861 ccccgcttcg ccgcgcgacc aaggccgccg ccgagatcga acggacccgc cacgaggtca
     1921 acgtcacgcg ccgcgaacaa cttgtgcaag agatctggtt cgccgtacgt gatgcggtac
     1981 gtaatcagcg cagcgcggcc cggcaggtcg aggccgccgc ccgcttccgc gagctcgccg
     2041 agaagagcct cgaggtcgag cagcggaagt tcctcaacgg tacgtcctcg aacttcttag
     2101 tagcccagcg tcaggaggag ctggcggcgg ctcagctatc cgagctgaca gcggtgctcg
     2161 gtcacaccaa agcggccgcc gccctcgcgc gtgcaaccgg tcaactcctc gacgaccgcc
     2221 acatccagct cggcgtgaag tgaactgtga gttgcagttg ttgagatgag ctgacaggag
     2281 caggttgctc atccaccccc gacccgcggt acaacggctg cggggatgac cgccgacctt
     2341 ccgatcgatg cgaagccatt cgacctgtgg ccgctcgtgc ccaagtcgat ccaggcgatc
     2401 atgccgaacg tgatgtggaa ccgggcccgg ctccagcggc tggacttgcc ggtccgagac
     2461 atcccggtcg cccagctgcg ctggcagctc gatctcccgt ggtggcggca tggcaacaag
     2521 gtcttcgcga tcacgccgaa ccaggtacgc aatgatccgg agacgttcgt catccagttc
     2581 cgccgcatgc tcgatgccga tctcgatttt ccgattcacg tgctcgagcg cgatcgcctg
     2641 atcctgctcg acggctttca ccgcctgctc aaggccgaca tgctcgagat gcgtacgatt
     2701 tcggcgcgcg tcgtcaacct cgcgcagttc gccgacgtcc tcgagcgccg ctgaaccgcc
     2761 ggccaccccc cgcgcatcga accgtcatga agcccgaatg gctcgtcgtt cgccaggatg
     2821 acaacggcaa cgaggtcgtc atggcggagg tcgcgacgga ggaagaggct cggtcgatcg
     2881 cgg
//
LOCUS       DASUFX010000426         6355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_763789,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000426 DASUFX010000000
VERSION     DASUFX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763789"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFV99_17050"
     CDS             <1..456
                     /locus_tag="VFV99_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061078.1"
                     /translation="YRQVFSGELALAERDWSRAEAIGNELARTARSQWLSAMPAVSAL
                     VDTLTATAELGIGTRAGAERARDRCRAMYRRVRYSFYAATALRLWGQAERALGNHASA
                     SRILSRAAAVASERGGKVDRLAVACLLGAKVDCGDLEFAVMWNTGGALE"
     gene            480..2246
                     /locus_tag="VFV99_17055"
     CDS             480..2246
                     /locus_tag="VFV99_17055"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5061079.1"
                     /translation="MIGGGVAGLTAAHELVERGFTVHVYETRPAWGGKARSQPVAGSG
                     TNGRKDLPGEHGFRFYPRFYTHVIDTMSRIPMPGTPHHVVDNLRPTTESAIALIDHDT
                     WFRFYRRKMTKPFDVLEALELFFQELDFDAEDMMLFGAKILQFLTTSDTRRLAEYDKI
                     SWWDFLEGDHYSQRFQRQLRAVPRTMVAMDPKRGSARTVGTISMQLILDYAEQGVNND
                     RTMGGPTSQMWFDPWITHLMSIGVQLHSGAQVDSLEMAGNKISGVKLAGGGTATADYY
                     VLAVPLDGVTNMITPQMAAADPVLQNLKNANLDQLVSWMVGIQYFLYEDVPLVRGHTF
                     YPDSPWALTTISQPQFWRDLGLFRRGYGNGEVGGLISVDISDWNTPGTFIPKTAKQCT
                     PDEIAKEVWEQLKAALNGSQPGEDILRDELKHSYHLDSDLDYSGGLPPKNTSRLLVHP
                     PGSWALRPEAGTAIGNLVLASDYVRTYTDLASMEGANEAARRAVNVILARSMSTAGKV
                     PTWPLEEPSYLEPWKRLDERLYHNGQSHLFELLGIRRAAQAADLLRRFAAFTGIAKLD
                     DLLDEVRATTIIKGILVRIGIS"
     gene            2262..2744
                     /locus_tag="VFV99_17060"
     CDS             2262..2744
                     /locus_tag="VFV99_17060"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5061080.1"
                     /translation="MSNETFEGGCDCREIRYRMTSRPLFVHCCHCRWCQRETGASFAL
                     NAMIEADRVQLLAGEVDVVLTPSNSGKGQKIARCPRCRIAVWSNYAGAGDAVRFVRVG
                     TLDNPDAFPPDIHIFTASKQPWVVLPPNMPAVPEYYDRTQYWPAESLARRDALLAKQR
                     "
     gene            complement(2746..3234)
                     /locus_tag="VFV99_17065"
     CDS             complement(2746..3234)
                     /locus_tag="VFV99_17065"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX5061081.1"
                     /translation="MTRTERRFLEGCRAGLIATLAMSFVMVLTYLIARRFFTEPVPLA
                     MIANLLARLFRSTTVTTPEVIAAWILHLAYGTFWFGLLAVSTYRVTWWKGLAVGIGLW
                     LIMLVFMLPLVGVPVFSLATWGWVWLATFVEHIVYGITGGAIMHRSDEFRRLPAVDME
                     ST"
     gene            complement(3322..4683)
                     /locus_tag="VFV99_17070"
     CDS             complement(3322..4683)
                     /locus_tag="VFV99_17070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5061082.1"
                     /translation="MAESIHRQLFRLAWPIIGLNVLQVMMLGVDSALCGRMANPQPVL
                     AALGYSIQVVFLLMVAMLGLTVGTVALVARAYGGGDTQRLNHLLVQATQLTVVVGIVV
                     GIAGALGAEHILLALDASPEVAKIGADYLRPLMAGTPFFYLTLLYAGILRGVGNTRIP
                     FLVALGTNVINAVLNYGLILGNLGMPALGVTGSAIGTVVAQASGVVALVMVLRSGKVP
                     NLKLPLHLRAVDHQLAVELFRVGWPAAIDLLVLNVGFLTALAMLGRIDQVSVAAHGLG
                     LRVQSFAFVPGIGIAQATGAMVGQALGGSNVPRAKAVARASILLCLAIMTALAIAIFA
                     TGPLLVQVFDVMPDTKLGDYTIVWLQILAVAMPPAAINMALIGLLQGAGATRTSLRIN
                     IWATIAIQVPVALVLGFGFDLGEIGVWWSFPIGFAAKAVLNYVELKRERWAVTGVRMS
                     KKV"
     gene            complement(4739..5872)
                     /locus_tag="VFV99_17075"
     CDS             complement(4739..5872)
                     /locus_tag="VFV99_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061083.1"
                     /translation="MRAVSLLVLVAVPAWARADVSVGASVGAGAQGAATYGALDLRLD
                     AAWPNIRLGIGARGVWDDAEFRRSDWNGVGSVLTLVRDFEATRDVGDTRFSVAAGALA
                     PAHITHVVNGYRAALDDRFHTGIRAAAEQTDLDAVLEVDDVLDPALIAGGLRFAMAPP
                     YAMHLGIAIDPSAPAMDGTHTITAIEAGASSRFEGFRARADVGVSVVAEISLGAGVVA
                     FANGAAERGGVLWTARADAQAGTGTAGSLFGPLYRIERLAHDGRASEWDRAHAGDLNG
                     ASVGFAVGAGIPTGWLELGARARPGLGGLVFASGGAPMGRYVQAGMWAAAGRDDAAGA
                     GELRVAWAKRMFSALQVARMYRLDPMEPISLWSVTAWFGATSQ"
     gene            complement(5936..6202)
                     /locus_tag="VFV99_17080"
     CDS             complement(5936..6202)
                     /locus_tag="VFV99_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061084.1"
                     /translation="MRRLVLLIVLAFAAPVAPAFGQQAPMTENPRAREQSDWKGPSGF
                     WTNPHPAKGGAYRWRMMAIGGGFLLVTGFLVLRLVKKTNAERGK"
BASE COUNT         1152 a         2210 c         1980 g         1013 t
ORIGIN      
        1 taccgtcagg tcttttccgg cgagctagcg cttgccgaac gcgattggtc gcgcgccgaa
       61 gcgatcggca acgagctcgc ccgcaccgcg cgttcgcagt ggctgtcggc gatgccggca
      121 gtctctgcac tcgtcgacac gctgaccgcc accgcggagc tcggcatcgg cacgcgcgca
      181 ggcgccgaac gagcacgcga ccggtgccgc gcgatgtatc gccgcgtgcg ctactcgttc
      241 tacgccgcga ccgcactgcg gctgtggggg caggccgagc gcgcgctcgg caatcacgca
      301 tccgcttcgc gcatcctctc gcgcgcagct gccgtcgcga gcgaacgcgg gggtaaggtc
      361 gatcgtcttg ctgtcgcgtg cctcctgggc gcgaaggtcg attgcggaga tcttgaattc
      421 gccgtcatgt ggaacacggg cggcgctctg gagtagcaat ggcaaatcct tccgtaatcg
      481 tgatcggcgg cggggtcgcc ggtctcactg ctgcgcacga gctcgtcgag cgcggcttca
      541 cggttcatgt ctacgagacg cgccccgcat ggggcggcaa ggcgcgttcg cagccagtcg
      601 ccggtagcgg cacgaacggc cgcaaggatc tgcccggcga gcacggcttc cgcttctacc
      661 cgcggttcta cacgcacgtg atcgacacga tgagccgcat cccgatgccg ggcacgccgc
      721 atcacgtcgt cgacaacttg cgcccgacaa ccgagagcgc gatcgccctg atcgatcacg
      781 acacgtggtt tcgcttctac cgccgcaaga tgacgaaacc gttcgacgtg ctcgaagcgc
      841 tcgagctgtt cttccaggag ctcgacttcg acgccgagga catgatgctg ttcggcgcga
      901 agatcctcca gttcctcacg acgagcgaca cgcgccggct cgccgagtac gacaagattt
      961 cgtggtggga cttcctcgag ggcgaccact actcgcagcg gttccagcgc cagctgcgcg
     1021 cggtgccgcg cacgatggtc gcgatggatc cgaagcgcgg atcggcgcga accgtcggca
     1081 cgatctcgat gcagctgatc ctcgactacg ccgagcaggg cgtgaacaac gaccgcacga
     1141 tgggcggtcc gacgagccag atgtggttcg acccgtggat cacacatctg atgtcgatcg
     1201 gcgtgcagct gcacagtggc gcgcaagtcg attcgctcga gatggccggc aacaagatca
     1261 gcggcgtgaa gctcgccggc ggcgggaccg caactgctga ctactacgtg ctcgccgtcc
     1321 cgctcgacgg cgtgacgaac atgatcacgc cgcagatggc agccgctgat ccggtgctgc
     1381 agaacctcaa gaacgcgaac ctcgatcagc tcgtgtcctg gatggttggc atccagtact
     1441 tcctctacga ggacgtgccg ctcgtccgcg gccacacgtt ctatccagac tcgccgtggg
     1501 cgctgaccac gatctcgcag ccgcagttct ggcgcgacct cggtctgttc cgccgcggtt
     1561 acggcaacgg cgaagtcggc ggcctgatct ccgtcgacat ctcggactgg aacacgccgg
     1621 gcacgttcat tcccaagacc gcgaagcagt gcacgcccga cgagatcgcg aaggaagtct
     1681 gggagcaact caaggccgcg ctcaacggtt cgcagcccgg cgaagacatc ctgcgcgacg
     1741 agctcaaaca ctcctatcac ctcgacagcg atctcgatta ctccggcggt ctaccgccga
     1801 agaacacgtc gcgcttgctc gtccatccgc cgggctcatg ggcgctgcgg cccgaagccg
     1861 gcactgcgat cggaaacctc gtgctcgcgt ccgattacgt gcgcacgtac accgatctcg
     1921 catcgatgga aggtgcgaat gaagcggcgc gccgcgcagt caacgtgatc ctcgcgcgct
     1981 cgatgtcgac tgccggcaag gtgccgacgt ggccgctcga ggagccgtcg tatctcgaac
     2041 cgtggaagcg gctcgacgag cggctctatc acaacggcca gtcacacctg ttcgagctgc
     2101 tcggcatccg gcgcgccgcg caagccgctg acctcttgcg tcggttcgcc gcgttcaccg
     2161 gcatcgcgaa gctggacgac ctgctcgatg aagtccgcgc cacgacgatc atcaaaggga
     2221 tcctcgtgcg catcggcatc tcgtgacata cgctcgctgc aatgagtaac gagacgtttg
     2281 aaggtggttg tgactgtcgc gagattcgct accggatgac atcgcggccg ctgttcgtgc
     2341 actgctgtca ttgtcggtgg tgtcagcgcg agaccggcgc gtcgttcgca ctcaacgcga
     2401 tgatcgaagc cgatcgcgta cagctgctcg ccggcgaagt cgacgtcgtc ctcacaccct
     2461 cgaatagcgg caagggccag aagatcgcgc gctgcccgcg ctgccggatc gcggtgtgga
     2521 gcaactatgc cggcgccggc gacgccgtgc gcttcgtgcg cgttggcacg ctcgacaacc
     2581 ccgacgcgtt tccgcccgac atccacatct tcacggcgtc gaagcagccg tgggtcgtgc
     2641 ttccgccgaa catgccggca gttcccgagt actacgatcg tacgcagtat tggcccgccg
     2701 agagtctcgc ccggcgcgac gcgctgctcg cgaagcagcg ttgacctacg tcgattccat
     2761 atcgaccgca ggcagccggc gaaactcgtc gctgcgatgc atgatcgcgc cacccgtgat
     2821 gccgtagacg atgtgctcca cgaacgtcgc gagccacacc cagccccagg tcgcgaggct
     2881 gaacaccggc accccgacga gcggcagcat gaacacgagc atgatcagcc agagcccgat
     2941 gccgacggcg aggcctttcc accacgtgac acggtacgtc gacaccgcga gcagaccgaa
     3001 ccagaacgtg ccgtacgcga ggtggaggat ccacgcagcg atcacctcag gtgtcgtcac
     3061 cgtcgtgctt ctgaacaacc tcgcgaggag gttcgcgatc atcgcgagcg gcaccggttc
     3121 cgtgaagaac cgtctcgcga tcaggtaggt gagtaccatc acgaagctca tcgcgagcgt
     3181 cgcgatcagt ccggcacgac agccttccag aaatcgtctc tcggtccgcg tcataggagc
     3241 tcctgatcca acgcgatgca ggccgcattc cgacgccgcg cgctcggaat ttcgccggcg
     3301 cgattatgtc tgagtcggcc gctagacctt cttcgacatc ctcacgccgg tcaccgccca
     3361 gcgctcgcgc ttcagctcta cgtaattgag cacggccttt gcggcaaacc cgatcgggaa
     3421 gctccaccac acgccgatct cgccgaggtc gaagccaaag ccgagcacga gcgcgaccgg
     3481 tacctggatc gcgatcgtcg cccagatgtt gatgcgcagg ctcgtgcgtg tcgcgccagc
     3541 accctgcagg aggccaatca gcgccatgtt gatcgcggct ggcggcatcg cgacggcgag
     3601 gatctgcaac cacacgatcg tgtagtcgcc gagcttcgtg tccggcatca cgtcgaacac
     3661 ctggacgagc agcgggcccg tcgcaaagat cgcgatcgcg agcgcggtca tgatcgcgag
     3721 gcacaagagg atcgacgcgc gcgcgaccgc cttggcccgt ggcacgttgc tgccgccgag
     3781 cgcctggccg accatcgcgc cggttgcttg cgcgatgccg atgcccggca caaacgcgaa
     3841 tgactgcacg cgcaaaccga ggccgtgcgc ggctacgctc acctgatcga tacggccgag
     3901 catcgcgagc gcggtgagaa aaccgacgtt gagcacgagc agatcgatcg ccgccggcca
     3961 gccgacgcga aatagctcga ccgcgagttg atgatcgacc gcgcgcaggt gcagcgggag
     4021 cttcaagttc ggcaccttgc cactgcgcag caccatcacg agcgcgacga cgccgctcgc
     4081 ctgcgcgacc acggtgccga tcgccgagcc ggtgacaccg agcgcgggca tgccgaggtt
     4141 gccaagaatc agcccgtagt tcagcaccgc gttgatcacg ttcgtgccga gcgcgacgag
     4201 aaatggaatc cgcgtgttgc caacgccgcg caagatgccg gcgtacaaga gcgtcagata
     4261 gaagaacggc gtgccggcca tcagcggccg cagataatcg gcgccgatct tcgccacttc
     4321 tggcgatgcg tcgagcgcga gcaggatgtg ctcggcaccg agcgcgccgg cgatgccgac
     4381 gacgataccg acgaccacgg tgagctgcgt cgcttgcacg agcaggtgat tgaggcgctg
     4441 cgtgtcgccg ccgccatagg cgcgcgcgac gagtgcgacg gtgccgacgg tcaagccgag
     4501 catcgcgacc atcagcagga acacgacctg gatcgagtag ccgagcgcgg ccaacaccgg
     4561 ctgcgggttc gccatccgtc cgcacagcgc cgagtcgacg ccgagcatca tcacttgtag
     4621 gacgttcaga ccgatgatcg gccaggcgag tcgaaacagc tgacgatgga tggactccgc
     4681 cacggccggc cagcctacag caaacgctgt accgactgca cccgccggtt tggtcaggtc
     4741 actggctggt cgcgccgaac cacgccgtca cgctccacag cgagatcggc tccatcggat
     4801 ccaaccgata catccgcgcc acttgcagcg cgctgaacat gcgctttgcc cacgcgacgc
     4861 gtagttcgcc cgcgcccgcc gcatcgtcgc gacctgccgc cgcccacatg ccagcttgta
     4921 cgtagcgccc catcggcgcg ccaccgctcg cgaacacgag gccaccgagg ccgggtcgcg
     4981 cccgggcgcc gagctcgagc cagcctgtcg gaattcctgc accgaccgca aacccgacac
     5041 tcgcgccgtt cagatcgcca gcatgtgcgc gatcccactc gctcgcgcga ccgtcgtgcg
     5101 cgagacgctc gatgcgatac agcggcccga acagcgagcc tgcggtgccg gtgcccgctt
     5161 gcgcatccgc gcgtgccgtc cacaagacgc cgccgcgctc cgccgcaccg ttcgcgaacg
     5221 cgacgacacc tgcaccgagc gaaatctcgg cgacgaccga cacgccgaca tcggcgcggg
     5281 cgcgaaaccc ttcgaagcga ctgctcgctc ctgcttcgat cgcagtgatc gtgtgcgtgc
     5341 catccatcgc aggcgcactc ggatcgatcg cgatcccgag atgcatcgcg tacggtggcg
     5401 ccatcgcgaa tcgcaaccca ccggcgatca acgccggatc gagcacgtca tcgacctcga
     5461 gcacggcgtc gagatccgtc tgctccgctg ccgcgcgaat gccggtgtga aaccgatcgt
     5521 cgagcgcggc gcgatagccg ttcaccacgt gcgtgatgtg cgccggcgcc agcgcaccag
     5581 ctgcaaccga gaacctcgtg tcgccgacat cgcgcgtcgc ttcgaaatca cgcacgagcg
     5641 tgaggacgct gccgacgcca ttccaatcgc tgcgccgaaa ctcggcgtcg tcccacacgc
     5701 cacgcgcacc gatgccgagc cggatgttcg gccacgccgc atcgaggcgc aggtcgagcg
     5761 cgccgtaggt cgctgcgccc tgtgcgcccg caccgacgga cgcgccaacg ctgacgtccg
     5821 cacgcgccca tgcgggcacg gcgacgagca ccagcaggga taccgctcgc atgcggcaaa
     5881 gcgtcgcccc gctcccggca cgggacaaaa ttttggcaat cgcgtgcgcc gctcgctatt
     5941 tgccgcgctc ggcgttcgtc tttttcacga gccgcagcac caaaaatcca gtcacgagca
     6001 gaaacccgcc gccgatcgcc atcatccgcc agcgatacgc cccgcccttc gccggatgcg
     6061 gattggtcca gaaccccgac gggcccttcc aatccgattg ctctctcgcg cgcgggttct
     6121 ccgtcatcgg cgcttgctgc ccgaaggcag gcgccacagg tgccgcaaac gcgagcacga
     6181 tgaggaggac cagacgacgc atcgaggatg tcttactcct tttcgcgcgg cttggaagcg
     6241 ccatcgcgct tgtcgccccg atcctttttg tcgcgcttgt ccttcttgtc gtgcttgtcg
     6301 ttcttgtcgg gaggcggatc gtcgtccttg gccatcggat tcgtcgaaac ggcgt
//
LOCUS       DASUFX010000427         7751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_347574,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000427 DASUFX010000000
VERSION     DASUFX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347574"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(565..912)
                     /locus_tag="VFV99_17085"
     CDS             complement(565..912)
                     /locus_tag="VFV99_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061085.1"
                     /translation="MSGQLNIPLMAPDQASKLLDEMVYERVLGFIDLDGLYDFERELW
                     CAYDEAGIADEDEVPKIVKAMIDKALMRFCDEPRRYMDFGPPFGAAPFDDCDLCAAEA
                     AAAAATKKKHKVS"
     gene            complement(909..1955)
                     /gene="recA"
                     /locus_tag="VFV99_17090"
     CDS             complement(909..1955)
                     /gene="recA"
                     /locus_tag="VFV99_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5061086.1"
                     /translation="MSAQEKKNRSIAEAVKAIHKQFGSGAIMRLDGSEVQQVEIIPTG
                     SVALDVALGCGGLPRGRIVEIYGPESSGKTTLTLHAIAEAQRAGGVCAFIDAEHALDT
                     EYARRLGVDLEDLLVSQPDCGEQALEICDTLVRTGAIDLIVVDSVAALTPRAEIEGDM
                     GDAHMGLQARLMSQALRKLTAVISRTRTCVIFINQLRQKIGVVYGNPETTTGGNALKF
                     YCSVRLDIRRKKAIKRGEETVGSEVKVKVVKNKLAPPFREAEFEILYGTGVNKLGELV
                     DTAEKLGLVEKSGTWYSYEGATLGQGRDKVMAHFDEHPALQQQLRTALVAQARRAAAG
                     AANPAANGAAGGVS"
     gene            complement(2059..3588)
                     /locus_tag="VFV99_17095"
     CDS             complement(2059..3588)
                     /locus_tag="VFV99_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061087.1"
                     /translation="MRWLALAPLVAATGCNWLYGLDATIAVDGASEGLPPGSRTRLVW
                     GIATTDGQAGTSGFDPEMVYKSIGSETARPQPPVVLVGDDSGLAEATYDPADGSFEIP
                     YALRESPHRVVYTLPGESVPHEVQWSITGAILVVPRTTRLDAPAPPTASGYSITPQGL
                     GTTLAMPALYTSGAFTATDSSSQFDQATAGTIKFAFPQNARPVTSPLGAPQKAKGDWV
                     LLVNFQPNGAQTSVNGFAMSQIDLAANMMLAPNPQPTWQTTTRTLSTICSTMPANCLP
                     NGNAAPAAQRLDAVLPGGTAGRKLFLGVSPSTDLPGFLPGVGPDFVDRPLMLTLHEST
                     QIDTTITLADPSSMLGLDRVIAARLTSARLANGAVLTSSIQTITNVFSGSLQYGAPLA
                     TNVKLGATSLSGTTESVPVGASSSQQTLSWSAEAGYVANDYVVTLYEITFSALVPVRI
                     YHVIAPEVKVDGNLLVAGHQYVFGITARTGFGAADRGDYQKAQYPFSVTTTFPRTFTV
                     Q"
     gene            3655..4377
                     /locus_tag="VFV99_17100"
     CDS             3655..4377
                     /locus_tag="VFV99_17100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX5061088.1"
                     /translation="MKLDARLNLFITLVAVFMTCLIVGDMIGGKLTSFELFGREWIFS
                     VGQLAFPVTFILTDILNEFYGRKVVRRITFLAFFMVGLTFCIIYAAAAMPWAPIALKS
                     DWTGVTPPQFSSVFTQATQIQLASMAAFLIGNLTDIWVFFLIKRLTGNRMLWLRSTGS
                     TAVSQLIDTIVISGIIWLRKVSFETYVTIVLTSYVIKLAAAIVVTPIIYGLHEVIEKK
                     YGIEPAPLEVASADVGGTDDAT"
     gene            complement(4374..5687)
                     /locus_tag="VFV99_17105"
     CDS             complement(4374..5687)
                     /locus_tag="VFV99_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061089.1"
                     /translation="MTRYLACALLLAACSEVHHGVDAGPADAAVDALPTSMGDPTPTS
                     VKLVVTRNGMPVVGVAVVFQNADSSLVSAQLTNDKGLAWTEMAAGGFVTALEHVGSGS
                     DELSTFAAVQPGDSLELAFAPAGPTDEWPVAITVPSDGSAVGYQVYTSCGGPIGLLPG
                     GTATATLLGCDGVADAVVVGEDDTSQPVSAFYAGNLTVPPAAEPPDFGSFAITGSYAP
                     LVTKTFSYANVPPLVSFIGTYHTFVSTHGRVYERTAGDTVSNGSVTTQVQLPATTAAS
                     LIVSTLFSSDVGSQTIYETSADASTPYAVDVGAAQLPPFATQPAYDVATRELAWTERS
                     GPQVPNLVRARIHVYRDDIPGGRAWNWRLVAPRTGASVTYPVLPSFGFDFNPKRGDAI
                     GLGELTTVDAPGGYANARRVGFSDLINLVGPGDKMLLETLTTMEP"
     gene            complement(5684..6460)
                     /locus_tag="VFV99_17110"
     CDS             complement(5684..6460)
                     /locus_tag="VFV99_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061090.1"
                     /translation="MLARFSLLALLAGCDLALGVSGQPDPCPLAKFANPNQDNVVEAD
                     LFSIDWDQQLAVIQRGGIAYEVTLTDKSETLIDLGPYSPASLALAPEGGALFYAVSIE
                     PLTLKGALRRGTAEWQLDAEVPRGTFAGTPSADAFGPRRVLVRMLSYSTTIQEYEVDE
                     HGHWETVGEPFDFAGDLAPNLTPNGLTMVYSAKDSLGQPGVYAASRDDVDSPFGKPFL
                     LRAGALQYPQLCGKCQQLYALVPNPDLASTNRSVLVRFDR"
     gene            complement(6444..7313)
                     /locus_tag="VFV99_17115"
     CDS             complement(6444..7313)
                     /locus_tag="VFV99_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061091.1"
                     /translation="MVLLLLLVSAAEAQPVDAPQQAPTTPDPTPAVAAFQEGRTLLEA
                     GKFADACAKFMVSIEADPEAPGTLLNLGLCNEMLGKIATALNWYRKAQFRAAETQMAE
                     YESVAKQHAFSLAAKVPTLQIDAAPGANVILDGAPVTEHDRARIEVDAGTHVIELGRE
                     RRTITLNEGDKQTIDLRPPPPKQFVVIDRGVTQRRYAYVAAGTGVAFWAASATLTLVA
                     KSKYDATDHPETYLHWQNVARFGGTSLFVVGSAAIATGVYLYVKAPRKERVEQVMPVV
                     GTNQVGVAVAGSF"
     gene            complement(7458..>7751)
                     /locus_tag="VFV99_17120"
     CDS             complement(7458..>7751)
                     /locus_tag="VFV99_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061092.1"
                     /translation="QVTEQYRADITTLCDVVHLSGADQKPKEEQWPIIAMFLGPALKT
                     NEAHEFLVAIQPLQAEPKALALETEAKRVGLAKCELANEWRSAASAPRGPSGS"
BASE COUNT         1277 a         2555 c         2511 g         1408 t
ORIGIN      
        1 gtcgagttcc tgtccaagac gaggcgcctt gcgctttgca aagccgaacc gaagctcggc
       61 ttctgcgacc acgctgatgc caatgtcact gcgatcgtgc tttagccaac gtgcgagcac
      121 cttcgggtgc aactgcatcg cgtagctaac gacgtcggtg tcgacgagga actgcatcat
      181 tgaagtcttt gaaaatgtgg tcgacagatt caggtggtcg atctcgcggc acttcgaagt
      241 cgagtggaac gtgaggtccc gaccacagcc aatcgatcca gtccttaggc caaccgccgt
      301 cgggttccag gattactttg tcgccttcgc gacgaatgaa gaccgtcttg ccttggaagc
      361 gaaactcctt cggcagtcgc actgcttgac tgcgtccggt ccagaacaac ttggcgcgaa
      421 caagcggctc gcgcgccggc tcctgtttcg aagcatgctt ttgcgtcgac gaggaccgcc
      481 gcagtttcgg cttcgtacgc atgatagcta tcatgatata taccatgccg ttccggcggc
      541 gtcctcgtcg agaaatcgct gcgctcagct gaccttgtgc ttcttcttgg tcgctgctgc
      601 agccgcggct tccgccgcgc acagatcgca gtcgtcgaac ggcgcagcgc cgaacggcgg
      661 accgaagtcc atgtaccggc gcggttcgtc gcagaagcgc atcagcgcct tgtcgatcat
      721 cgccttgacg atcttcggca cctcgtcctc gtcggcgatc ccggcctcgt cgtacgcgca
      781 ccacagctcg cgctcgaagt cgtacagccc gtcgagatcg atgaacccga gcacgcgctc
      841 gtagaccatc tcgtcgagca acttcgaggc ctggtcgggc gccatcagcg gaatgttcag
      901 ctggccgctc acgacacacc tcccgccgcg ccattcgctg ccgggttcgc cgcgcctgct
      961 gccgcacgcc gcgcctgcgc gaccaacgcc gtgcggagct gctgctgcaa cgccggatgc
     1021 tcgtcgaagt gcgccatcac cttgtcgcgt ccctggccga gcgtcgcgcc ttcgtacgag
     1081 taccaggtgc cactcttctc gacgagaccg agcttctccg ccgtgtcgac gagctcgccg
     1141 agcttgttga cgccagtgcc gtacaggatc tcgaactccg cttcgcggaa cggcggcgcg
     1201 agcttgttct tcacgacctt caccttgacc tcgctaccga cggtctcttc gccgcgcttg
     1261 attgccttct tgcgccggat gtcgaggcgc accgagcagt agaacttgag cgcgttgccg
     1321 ccggtcgtgg tctccgggtt gccgtagacg acgccgatct tctggcgcag ctggttgatg
     1381 aagatgacgc acgtacgcgt gcgcgagatg accgcggtga gcttgcgcag cgcctgactc
     1441 atcagccgcg cttgcaagcc catgtgcgca tcgcccatgt cgccttcgat ctcggcacgc
     1501 ggcgtgagcg cggcaaccga atcgacgacg atcagatcga tcgcaccggt acgcacgagc
     1561 gtgtcgcaga tctcgagcgc ttgctcgccg caatcgggct gcgacacgag cagatcctcg
     1621 aggtcgacgc cgagccggcg tgcgtactcg gtatcgagcg cgtgctcggc atcgatgaac
     1681 gcgcacacac cgccggcgcg ctgtgcctcc gcgatcgcgt gcaacgtcaa cgtcgtcttg
     1741 cccgacgact ccgggccgta gatctcgacg atgcgtccac gcggcaagcc accgcagccg
     1801 agcgcgacgt cgagtgcgac cgagccggtc gggatgatct cgacctgctg cacttcgctg
     1861 ccgtcgagcc gcatgatcgc gccggacccg aattgtttgt ggatcgcttt gaccgcctcg
     1921 gcgatcgagc ggttcttctt ctcttgagcc gacataaaac ctccgcgccg gcccattcca
     1981 catcgcgtgc cgcgccaagc tcgcgaattg tcgcgtgcgc gtgtccggag tgtccggagg
     2041 tcggacttcg ccggacgcct actggacggt gaacgtacgc gggaacgtcg tcgtcacgct
     2101 gaacggatac tgcgccttct ggtagtcgcc gcggtcggct gcgccgaagc ccgtgcgcgc
     2161 cgtgatgccg aagacgtact ggtgaccagc gacaaggagg ttgccgtcga ctttcacttc
     2221 tggcgcgatc acgtgataga tgcgcaccgg gacgagcgcg gagaacgtga tctcgtacag
     2281 cgtcacgacg tagtcgttcg cgacgtaccc agcttcagcg ctccagctga gcgtctgctg
     2341 actcgacgat gcgcccaccg gcacactctc ggtcgtgccg gataggctcg tcgcgccgag
     2401 cttcacgttc gtcgcgagcg gggcaccgta ctgcaacgag ccgctgaaca cgttcgtgat
     2461 ggtctggatc gaggacgtca gaaccgcgcc gttcgccaac cgtgccgagg tcagccgcgc
     2521 agcgatcacg cgatcgagcc cgagcatgct gctcggatcg gcgagcgtga tcgtggtgtc
     2581 gatctgggtc gactcgtgca gcgtgagcat caacggccgg tcaacgaagt ccggcccgac
     2641 gccgggcaag aagccgggga ggtcggtgct cggcgatacg ccgaggaaca attttcgtcc
     2701 cgccgtacca cccggtagga ccgcgtcgag ccgctgtgcg gccggcgccg cattgccgtt
     2761 cggcaagcag ttcgcgggca tcgtcgaaca gatcgtcgac aacgtgcgcg tggtcgtctg
     2821 ccacgtcggc tgtggattcg gtgccagcat catgttcgcg gcaagatcga tctggctcat
     2881 cgcgaaacca ttgaccgacg tctgcgcccc gttgggctgg aagttgacca gcagcaccca
     2941 gtcacctttc gctttctgcg gggcgccgag cggcgacgtg acgggccgcg cgttctgcgg
     3001 gaacgcgaac ttgatcgttc ctgccgtcgc ctggtcgaac tgagacgacg aatcggtcgc
     3061 cgtgaatgcg ccagacgtgt acagcgcggg catagcgagc gtcgtgccga gcccttgcgg
     3121 cgtgatcgag tagccgcttg ccgtcggtgg cgcgggcgca tcgagacgcg tcgtgcgcgg
     3181 cacgaccagg atcgcgccgg tgatcgacca ctgcacttcg tgcggcaccg actcgcccgg
     3241 cagcgtgtag accacgcgat gtggcgactc gcgcagcgcg tacggaatct cgaacgagcc
     3301 gtctgcggga tcgtacgtcg cctctgcgag accgctgtcg tcgccgacga gcacgaccgg
     3361 cggttgcggc cgcgcggtct cgctaccgat cgacttgtag accatctcgg ggtcgaagcc
     3421 gctcgtgccg gcctggccgt cggtcgtcgc gatgccccac acgagtctcg tgcgcgagcc
     3481 gggtggtaat ccttctgagg cgccgtcgac cgcaatggtg gcgtcgaggc catagagcca
     3541 gttacatccc gtcgccgcga cgagcggtgc gagggcaagc cacctcatcc gggcgaatcg
     3601 tggcatggcg cccgcgttgc acacaagttc gagcggccat gccaggatgc gcgtgtgaag
     3661 ttagacgcac ggctcaacct cttcatcacg ctcgtcgccg tgttcatgac gtgcctcatc
     3721 gtcggcgaca tgatcggcgg caagctgacg tcgttcgagc tgttcggccg cgagtggatc
     3781 ttctccgtcg gtcagctcgc gtttcccgtc acgttcatcc tcaccgacat cctgaacgag
     3841 ttctacggcc gaaaagtcgt acgcaggatc acgttccttg cgttcttcat ggtcggtctg
     3901 accttctgca tcatctatgc ggcggctgcg atgccgtggg caccgatcgc gctgaagtcc
     3961 gattggaccg gcgtcacgcc gccccagttc agcagcgtgt tcacgcaggc cacgcagatc
     4021 cagctcgcgt cgatggcggc gttcttgatc ggcaacctga ccgacatctg ggtcttcttc
     4081 ctgatcaaga ggctgaccgg caatcgcatg ctgtggcttc gctcgactgg ttcgaccgct
     4141 gtctcgcagc tgatcgacac gatcgtgatc tccggcatca tctggctgcg caaggtctcg
     4201 ttcgagacct acgtcaccat cgtgctcacg tcgtacgtca tcaagctcgc cgcggcgatc
     4261 gtcgtgaccc cgatcatcta tggtctgcac gaggtgatcg agaagaagta cggcatcgag
     4321 ccggcaccgc tcgaagtcgc gagcgccgac gtcggtggta ccgacgacgc gacctagggc
     4381 tccatcgtcg tcagcgtctc gagcagcatc ttgtcgccgg gaccgacgag gttgatgagg
     4441 tccgagaatc cgacgcgccg cgcattcgcg tagccacctg gtgcgtcgac ggtcgtcagc
     4501 tcgccaagtc cgatcgcgtc gccgcgcttg ggattgaaat cgaaaccgaa cgacggcagc
     4561 accggatacg tcacgctcgc gccagtgcgt ggcgcgacga gtcgccagtt ccacgcgcgg
     4621 ccgcccggaa tgtcgtcgcg atagacgtgg atgcgggcgc gcacgagatt cggtacctgt
     4681 ggccccgacc gctcggtcca cgcgagctcg cgcgtcgcga cgtcgtatgc cggttgcgtc
     4741 gcgaacggcg gtagctgcgc ggcgccgacg tcgaccgcat acggcgtgga cgcatcggcg
     4801 ctcgtctcgt agatggtctg gctgccgacg tcgctcgaga acagcgtcga cacgatcagc
     4861 gacgcggcgg tcgtcgcggg cagctgcacc tgcgtcgtca cgctgccgtt actgacggta
     4921 tcgcccgcgg tgcgctcgta gacccggccg tgtgtgctta cgaacgtgtg gtacgtgccg
     4981 atgaacgaga ccagcggtgg cacgttcgca tacgagaacg tcttcgtgac gagcggggcg
     5041 tagctgccgg tgatcgcgaa cgagccgaag tccggcggct ccgctgccgg cggcacggtg
     5101 agattgccgg cgtagaacgc gctgaccggc tgcgacgtgt cgtcctcgcc gacgacgact
     5161 gcatcggcga cgccgtcgca gccgagcagc gtcgcggtgg cggtaccacc aggcaagagc
     5221 ccgatcggac cgccgcacga cgtgtagacc tgatagccga ccgcactgcc atccgacggc
     5281 acggtgatcg ccaccggcca ctcgtcggtc gggcccgctg gcgcgaacgc gagctcgagc
     5341 gaatcgccgg gctgtacggc ggcaaacgtc gacagctcgt cgctgccgct accgacgtgc
     5401 tcgagcgcgg tgacaaaacc gccggccgcc atttccgtcc acgcgaggcc cttgtcgttc
     5461 gtcaactgcg cgctgacgag cgaggaatcg gcgttctgga acacgaccgc gacgccgacg
     5521 accggcatgc cgttgcgcgt gacgacgagc ttgaccgacg tcggtgtcgg atcacccatc
     5581 gacgtaggga gcgcgtcgac cgcggcatcc gcgggccccg catcgacgcc atgatgtacc
     5641 tcgctacaag cagcgagcag cagcgcgcat gcgaggtaac gagtcatcga tcgaacctca
     5701 ccagcacgct gcgattggtc gacgcgagat cggggtttgg cacgagcgcg tagagctgct
     5761 ggcacttgcc gcacagctgc gggtactgca gcgcgccggc gcgcaacagg aacggtttgc
     5821 cgaacgggct atcgacatcg tcgcgcgatg ccgcgtagac gccaggctgg ccgagcgagt
     5881 ctttcgccga gtagaccatc gtgagcccgt tgggcgtgag gttcggcgcg agatcgccgg
     5941 cgaagtcgaa cggctcgccg acggtctccc agtgcccgtg ttcgtcgacc tcgtactcct
     6001 ggatcgtcgt cgaatagctc agcatgcgca cgagcacgcg gcgtggtccg aatgcatcgg
     6061 cgctcggcgt gcccgcaaac gtgccgcgcg gaacttctgc gtcgagctgc cactcggcgg
     6121 tgccgcggcg aagcgcgccc ttgagcgtga gcggctcgat cgacaccgcg tagaacagcg
     6181 cgccgccttc gggcgcgagc gcgagtgacg ccggcgagta cggcccgaga tcgatcagag
     6241 tctcgctctt gtcggtcaac gtgacttcgt acgcgatccc accacgctgg atcacggcga
     6301 gctgctggtc ccagtcgatc gagaacagat cggcttcgac gacgttgtcc tggttcgggt
     6361 tcgcgaactt cgcgagcggg cacggatcgg gctggcccga aacgccgagc gcgagatcgc
     6421 aaccggcgag caacgcgagc aggctaaaac gagccagcaa cggccacccc cacctggttc
     6481 gtaccgacga ccggcatcac ttgctcgacg cgctccttgc gcggcgcttt gacgtacaag
     6541 tacacgccgg tcgcgatcgc agcgctaccg acgacgaaca gcgacgtccc gccgaaccgc
     6601 gcgacgttct gccagtgcag atacgtctcg ggatgatcgg tcgcgtcgta cttgctcttg
     6661 gcgacgagcg tgagcgtcgc gcttgccgcc caaaacgcca ccccagttcc agccgcaacg
     6721 tacgcgtagc gccgctgcgt gacgccgcga tcgatcacga cgaactgctt cggcggcggc
     6781 gggcgtagat cgatcgtctg cttgtcaccc tcgttcaggg tgatggtgcg gcgttcgcgg
     6841 ccgagctcga tcacgtgcgt tccggcatcg acctcgatgc gcgcgcggtc gtgctcggtg
     6901 acgggcgcgc catcgagaat gacgttggcg cctggagcgg catcgatctg gagcgttggc
     6961 actttcgcgg cgagcgagaa cgcgtgctgc tttgcgaccg attcgtactc ggccatttgc
     7021 gtttcggccg cgcgaaactg cgccttgcga taccagttga gcgcggtcgc gatcttgccg
     7081 agcatctcgt tgcacaggcc gaggttcagc agcgtgccgg gtgcttccgg atcggcctcg
     7141 atcgagacca tgaacttcgc gcacgcgtct gcgaacttgc cggcctcgag cagcgtgcgt
     7201 ccctcctgga acgcggccac cgctggcgtc gggtcgggcg tggttggagc ctgctggggt
     7261 gcgtccacag gctgcgcctc tgcagccgac acgagcagga gcaggagcac caccgcgcgc
     7321 accgttcacc agtctaacat ccgtatgcgt gcggctcctc gcgtttcgac ggcgaggtgc
     7381 ccgaagggga agcacgtaca cgtcgggcgt acggcggctg cacagcgctt gcgagacgct
     7441 gcgttccagc agatttctca gctacccgat gggccgcgtg gcgccgacgc agcggagcgc
     7501 cattcgttgg ctagctcgca tttcgcgaga cctacacgct tggcttcggt ttcgagcgcg
     7561 agcgccttcg gctcggcttg cagcggctgg attgcgacga ggaactcatg agcctcgttt
     7621 gtcttcagcg ccgggcccag aaacatcgcg atgatcggcc actgctcctc tttgggtttc
     7681 tggtcggcgc ccgacaggtg caccacatca cagagggtgg tgatgtcggc tcggtactgc
     7741 tctgtgacct g
//
LOCUS       DASUFX010000428         6269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1874918, whole genome shotgun sequence.
ACCESSION   DASUFX010000428 DASUFX010000000
VERSION     DASUFX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874918"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..922)
                     /locus_tag="VFV99_17125"
     CDS             complement(5..922)
                     /locus_tag="VFV99_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX5061093.1"
                     /translation="MSNKLLLAALFSVAFGNVAVADDGPTTAGAGRRLNSLHTDKAVR
                     HRRLLVKNRFEVSPLFESTINPEFQHIVGGGLKLEYHLSDMLSIGAVGVGSTQFNTKL
                     TDRIVPTLESDTNTATREPSQQEFTDRLNQMPVHGAAFVSITPWYGKLAAFSQAFVAF
                     DFYFQAGVSYAQLTSKWDAPPCTPTSTMTTFCEDPNPGKPRPAAMPGETIPADNNPNN
                     DNPQNDGTRVGLYLGGGIHVFLSDFLALDLSVRDYAFSDNPSGADYNADLFVGKNDAT
                     GDDDNRFLHHLFFGAGISIMFPTTVKRTP"
     gene            complement(939..1868)
                     /locus_tag="VFV99_17130"
     CDS             complement(939..1868)
                     /locus_tag="VFV99_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX5061094.1"
                     /translation="MASTAVGQPKKGGSAPKGGTAAPKGGGSGSAAGGADEGIEIDQP
                     AAGSGDSGTIDMEQEEKPQGSLEADLQANDQVTAVKTGPVKKTPISWKDILVVVRKPF
                     LKLKRTELFPFTGITMNDNMITHYTIGGEFAYWMTDVLAIGVEAQYYQHSFGEPFDLV
                     ARQARRLPTVNQYNWSSALNFHYVPVYGKFAVLDKKLVAWEVAFTAGVGAGQSEVIPR
                     DTKFPGFTTILIQPNVGASMRFFLAKWLTIQAGIRDYMFIDKFEPTDRSPTMNASASE
                     AKDNADSAFINNIMFQIGVSFWLPTSFEYTTFR"
     gene            complement(2021..3220)
                     /gene="ftsY"
                     /locus_tag="VFV99_17135"
     CDS             complement(2021..3220)
                     /gene="ftsY"
                     /locus_tag="VFV99_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5061095.1"
                     /translation="MQTELIHFGIAIVVVILVLLWLRVASKRSRTTGPTDTRARLGLE
                     EKPEAPVRKRPVKTAKKPEAVPEAEKRKAEQEEKAEEEEVAAVTDEETAAAYKAGLAK
                     TRGGFVARLGKLFGKKQIDAATVDELEEVLFTADIGPRAAERIFQSVKGGLSKADLED
                     SDKIWNQIKKTSREILAVNEKPFDLPANKPFVLLVLGVNGVGKTTTIGKLASLWKGQG
                     KKVLLAAGDTFRAAATEQLEEWAKRAGVDIVKGKAGGDPSSVVFDAIKKGVDDSYDIV
                     ICDTAGRLHSNAGLMDELKKLRRVSDKACPGAPHETWMVLDATTGQNAISQAKTFKAD
                     MEISGIVLTKLDGTSKGGVVLGICDELKVPVRFVGLGEKITDLRPFDHKAFVEKLYER
                     ADTSEAA"
     gene            complement(3239..4903)
                     /locus_tag="VFV99_17140"
     CDS             complement(3239..4903)
                     /locus_tag="VFV99_17140"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5061096.1"
                     /translation="MRLPALLALVVLVQLAEAGPTVEIRAQTQLSLGKVKKRDGGMVE
                     VTGHLNDKLTGTGIAGASVSVSIGNFSNTGTTGEDGSFAIELPGDDGPQEVRLEYQGG
                     RSLEKAVPLTVTTDPAKQQLELEIAMIGPDPAGAKLKINAKGDDGGAFVPVKLEIGTV
                     DSQKLRSLRTVDSGTEFVLARKDVGGAGQYRLRATFVGDDMRAGAIATSTVMLDSNTT
                     TSMNLSTTKLAFEDELVITGKVLDDDGAPVEHAAVTLVSGDRRLAQAATTDRGTYRFE
                     VEGEIIGQGQFGIQVQSDPGKPFIRSSRSNPELVRVAAPAPVPVSYTIAAFLATAIAA
                     GGFFAARNKPWRRLRRPAPPAEVPSQEGEIEQLQGGLVVAKPGVVATLRRPHDDGFSG
                     VVRDTVRGRPVVDAVVILVLGEQERSIRTQADGSFTIDKLPVGEWRAEVAAPGYVTER
                     FAVTIPHRGELRGVRIDLVPVRERVFQLYRRAAEPVLPETRLWGIWSPRQIVDHVRSK
                     RPSPALADLTDFVEEIYFSPRLAAETVLSQASEQVDRAIHERAARR"
     gene            4981..>6269
                     /locus_tag="VFV99_17145"
     CDS             4981..>6269
                     /locus_tag="VFV99_17145"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5061097.1"
                     /translation="MRDLSRRDLLVGIGAAAAAGAVGCSGDHTGAQPDATASGDDAPA
                     EPDASSVNACTAASSMTAEQLLAHIETIVVLCMENRSFDHYLGSLRLVEGRADIDGLT
                     GSEMNMSTANVAVPVHQLDDFTPADPPHGWDACHAQWNGGANDGFVKEHAGSSEADVM
                     GYHVRSQIPTTYALADAAAICHRWFAGCLGPTWPNRFFLHGGTSNGVKSNLPALGFTS
                     IFDRLAQKNITATNYYSDVAWASGGYGKLLGLSRIEQFFEQAMAGTLPQFSIIDPAFT
                     GGGANDDHPDHDIHMGQALIASIVAALGQSPQWSKCLFVITYDEHGGFFDHVPPPSAV
                     DDNAEFQQLGFRVPALVLGPTVRRGCAIDTVFDHSSVIATATKRWGLAPMTMRSQAAN
                     DLSPCIDPLRIGNPLPPPVLPSVKVSQSRLAERQAKA"
BASE COUNT          998 a         2014 c         2070 g         1187 t
ORIGIN      
        1 cagatcaggg cgtccgcttc acggtcgtcg ggaacatgat cgagataccc gcaccgaaga
       61 acaggtggtg caggaagcgg ttgtcgtcgt cgccggtggc gtcgttctta ccgacgaaca
      121 ggtccgcgtt gtagtcggcg cccgacgggt tgtcggaaaa cgcgtagtcg cgcaccgaga
      181 ggtcgagcgc gaggaagtcg ctcaggaaga cgtggatgcc gccgccgagg tacaggccga
      241 cgcgcgtgcc gtcgttctgc gggttgtcgt tgttcgggtt gttgtcggcg ggaatcgtct
      301 cgccgggcat cgcggcgggg cgcggcttac cgggattcgg gtcctcgcag aacgtcgtca
      361 tcgtgctcgt cggcgtgcac ggcggcgcat cccactttga cgtgagctgc gcgtacgaga
      421 cgccggcctg gaagtaaaag tcgaacgcca cgaacgcttg gctgaacgcg gcgagcttgc
      481 cgtaccacgg cgtgatgctc acgaacgcgg cgccgtggac cggcatctgg ttcaagcggt
      541 cggtgaactc ttgttggctc ggctcgcgcg tcgccgtgtt cgtatccgac tcgagcgtcg
      601 gcacgatgcg atcggtgagc ttggtgttga actgcgtcga gccaacaccg acagcaccga
      661 tcgagagcat gtcgctcagg tggtactcga gcttcagacc accgccgacg atgtgctgga
      721 actcggggtt gatcgtcgac tcgaacagtg gcgacacctc gaagcggttc ttgacgagga
      781 ggcggcggtg acggacggcc ttgtccgtgt gtagcgagtt gaggcggcgg cctgcacccg
      841 cagtcgtcgg accgtcgtcg gcgacggcga catttccaaa cgccacgctg aagagcgcgg
      901 ccagaagcag cttgttgctc atgctggtct ccatctcctc agcggaacgt cgtgtactcg
      961 aagctggtcg gcagccagaa gctgacgccg atctggaaca tgatgttgtt gatgaacgcg
     1021 ctgtccgcgt tgtccttcgc ctcgctcgcg ctcgcgttca tggtcggcga acgatccgtc
     1081 ggctcgaact tgtcgatgaa catgtagtcg cgaatgccgg cctggatggt gagccacttc
     1141 gcgaggaaga agcgcatact cgcaccgacg ttcggctgga tcaggatcgt cgtgaagccg
     1201 gggaacttgg tgtcacgcgg aatcacttcc gactgacccg cgccgacgcc ggcggtgaac
     1261 gccacctccc acgcgacgag cttcttgtcg agcactgcga attttccgta gaccggcacg
     1321 tagtggaagt tcagcgcgct ggaccagttg tactggttca ccgtcggcag gcgacgcgcc
     1381 tggcgagcga cgaggtcgaa cggctcgccg aacgagtgct ggtagtactg cgcctcgaca
     1441 ccgatcgcga ggacgtcggt catccagtac gcgaactcac caccgatcgt gtagtgagtg
     1501 atcatgttgt cgttcatcgt gatgccggtg aacgggaaca gctcggtgcg cttgagcttc
     1561 aagaacggct tgcgcacgac cacgaggatg tccttccacg agatcggcgt tttcttcacc
     1621 gggccggtct tgaccgcggt gacctgatcg ttggcctgga ggtcggcttc gagagagcct
     1681 tgcggcttct cttcctgctc catgtcgatc gtgccgctgt cgccgcttcc ggcagcgggc
     1741 tggtcgattt cgatgccttc atcggccccg ccggcggcgc ttccgctgcc acctcccttg
     1801 ggagcagcag tgcctccctt cggcgcggag ccaccttttt tcggctggcc gaccgcggta
     1861 ctcgccattg cgagtccaag ggcgacgacc aataaggacg ctgttcttcc cctgaaggtg
     1921 cgcattcgta tcggcttcat aacacagccc tttttcccat ctcaagcaaa ccgcttaggt
     1981 ttggttgagc ggagacacat ttgtgcgaag ccgattgcga tcaggcggct tcgctggtat
     2041 cggcgcgttc gtacagtttc tcgacgaatg ctttgtgatc gaacgggcgc agatccgtta
     2101 ttttttcgcc gagcccgacg aatctgacag gaacttttag ctcgtcgcag ataccgagga
     2161 cgacgccacc cttcgaggtg ccgtcgagct tggtcagcac gatgcccgag atctccatgt
     2221 cggccttgaa ggttttcgcc tgggagatcg cgttctgccc cgtcgtggcg tcgaggacca
     2281 tccaggtttc atgcggcgca cctgggcacg ccttgtcgga cacgcggcgc agcttcttga
     2341 gctcgtccat gaggcccgcg ttcgagtgca agcgaccggc ggtatcgcag atgacgatgt
     2401 cgtagctgtc gtcgacgccc ttcttgatcg cgtcgaacac gacgctcgac ggatcgccgc
     2461 ccgctttgcc tttgacgatg tcgacgccgg cgcgcttcgc ccattcctcg agctgctcgg
     2521 tcgcggccgc gcggaacgtg tcgcccgcgg cgagcagaac cttcttgcct tggcctttcc
     2581 acagcgacgc gagcttgccg atcgtcgtcg tcttgccgac gccgttgacg ccgagcacga
     2641 gcaacacgaa cggtttgttc gccggtaggt cgaacggctt ctcgttgacc gccaagatct
     2701 cgcgcgacgt ctttttgatc tggttccaga tcttgtcgct gtcttcgaga tccgccttcg
     2761 acaggccgcc cttcaccgac tgaaagatgc gctccgcggc gcgcggtccg atgtcggcgg
     2821 tgaacagcac ctcttcgagc tcgtcgacag tggcggcgtc gatctgcttc ttgccgaaca
     2881 gcttgccaag ccttgcgacg aagccgccgc gcgtcttcgc gaggcccgcc ttgtacgcag
     2941 cagcggtttc ctcgtcggtg accgccgcaa cctcttcctc ctcggctttc tcttcctgct
     3001 ctgctttgcg cttctcggcc tcgggaacgg cttccggctt cttcgcggtc ttgacgggcc
     3061 gcttgcggac cggtgcttcg ggtttctctt cgaggccaag ccgggcgcgc gtgtcggtcg
     3121 gtccggtggt gcggctgcgc ttcgacgcga cccgcaacca caggagcacg agtatgacca
     3181 cgacaatcgc gatcccgaaa tggatcagct ctgtctgcat cgagcgctgt ataccgtatt
     3241 agcgacgcgc tgcgcgctcg tggatcgcgc ggtcgacctg ctcggatgct tgggacagca
     3301 ccgtctcggc cgcgagccgc ggcgagaagt agatttcttc gacgaaatcg gtgaggtcgg
     3361 cgagggcagg gctgggccgc ttgctgcgca cgtgatcgac gatctggcgc ggtgaccaaa
     3421 tgccccacag ccgcgtttcc gggagtaccg gctcggcagc gcggcggtag agctggaaca
     3481 cgcgctcgcg cacgggcacc aggtcgatgc ggacgccgcg gagctcgccg cgatgcggaa
     3541 tcgtgaccgc aaagcgctcg gtgacgtatc cgggcgccgc gacctccgcg cgccactcgc
     3601 cgacggggag cttgtcgatc gtgaagctgc cgtcggcctg cgtgcggatc gagcgctcct
     3661 gctcgccgag cacgaggatg acgacggcgt cgacgacagg gcggccccgc acggtgtcgc
     3721 gcacgacgcc cgagaagccg tcatcgtgtg gacggcgcag cgtcgcgacg acgccgggct
     3781 tggcgacgac gaggccgcct tgcagctgct cgatctcgcc ttcctggctc ggcacctcgg
     3841 cgggcggcgc aggacggcgc agccggcgcc acggtttgtt gcgcgcggcg aagaagccac
     3901 cggctgcaat cgcggtcgcg aggaacgccg cgatcgtgta cgagactggc accggcgccg
     3961 gcgcagcgac acgcacgagc tctggattgc tgcggctcga gcgaatgaac ggcttgcccg
     4021 gatcggactg gacctggatg ccgaactggc cctgaccgat gatctcgccc tcgacctcga
     4081 agcgatacgt gccgcggtcg gtggttgctg cttgcgcgag ccggcgatcg ccggacacga
     4141 gcgtgaccgc ggcgtgctcg accggcgcac cgtcgtcgtc gagaaccttg ccggtgatga
     4201 cgagctcgtc ctcgaacgcg agcttggtcg tcgacaggtt catcgacgtc gtcgtgttcg
     4261 agtcgagcat cacggtactc gtagcgatcg ctcctgcgcg catgtcatcg ccgacgaagg
     4321 tcgcgcgcag ccggtattga ccggcgccgc cgacgtcctt gcgcgcgagc acgaattcgg
     4381 tgccgctatc gacggtgcgc agcgaccgca gcttctgtga atcgaccgtg ccgatttcga
     4441 gcttcaccgg cacgaacgcg ccgccgtcgt cgcccttcgc gttgatcttg agcttcgcgc
     4501 cggccgggtc gggaccgatc atcgcgatct cgagctcgag ctgctgcttc gccgggtcgg
     4561 tcgtgacggt cagcggcacg gccttctcga ggctacggcc accttggtac tcgagccgga
     4621 cctcctgcgg gccgtcgtcg cccgggagct cgatcgcgaa cgagccgtcc tctccggtgg
     4681 tccccgtatt cgagaagtta ccgatcgaca ccgagaccga cgccccggcg atgccggtgc
     4741 cggtcaactt gtcgttgagg tggccggtga cctcgaccat cccaccgtcc cgcttcttca
     4801 ccttgccgag cgagagctgg gtctgcgcac ggatctcgac ggtcgggcct gcttcagcaa
     4861 gctgtaccag gaccaccagg gcgagcagcg ccggaagacg catgtaacag gtgatagcac
     4921 gcgcatcgat tgtcgttgcg accgtcggtg accggcgtga tagtccgcgc cggagggacg
     4981 atgcgcgatc tatcgcgtcg cgatttgctg gtcggtattg gcgcagccgc tgcggcgggc
     5041 gcggtcggct gcagtggcga tcatacgggt gcacaaccgg atgcgaccgc atcgggggac
     5101 gatgcacccg cagagccgga tgcctcgagc gtgaacgcgt gcacggcggc gtcgtcgatg
     5161 acggccgagc agctgctcgc tcacatcgaa acgatcgtcg tcctctgcat ggagaaccga
     5221 tcgttcgatc attatctggg ctcgctgcgg ctcgtcgaag gtcgcgccga catcgacggg
     5281 ctcaccggca gcgagatgaa catgtccacc gcgaacgtcg cggttccggt gcaccagctc
     5341 gacgacttca cgcccgccga tccgccgcac ggctgggatg cgtgtcacgc gcagtggaac
     5401 ggcggtgcga acgacggctt cgtgaaggag cacgccggca gctccgaagc ggacgtgatg
     5461 ggctatcacg tgcgcagcca gatcccgacg acgtatgcgc tcgccgacgc ggccgcgatc
     5521 tgtcatcgct ggttcgcggg atgcctcggc ccgacgtggc cgaatcggtt cttcctgcac
     5581 ggcggaacgt cgaatggcgt caagtcgaac cttcccgcgc tcggcttcac gagcatcttc
     5641 gatcggctcg cgcagaagaa catcactgcg acgaactact actccgatgt cgcgtgggca
     5701 tcgggtggat acggcaagct gctcggcctc tcgcggatcg aacagttctt cgagcaagcg
     5761 atggcaggca cgctgccgca gttctcgatc atcgatccgg cgttcaccgg tggcggcgcc
     5821 aacgacgatc atcccgatca cgacatccac atgggccagg cgctcatcgc gagcatcgtc
     5881 gctgcgctcg gccagtcgcc gcagtggtcg aagtgtttgt tcgtgatcac gtatgacgag
     5941 cacggtgggt tcttcgatca cgtgccgccg ccgagcgcgg tcgacgacaa cgccgagttc
     6001 cagcagctcg gcttccgcgt gcccgcgctc gtgctcgggc cgaccgtgcg gcgcggctgc
     6061 gcgatcgata ccgtgttcga tcacagctcg gtgatcgcga ccgcgaccaa gcggtggggc
     6121 ctcgcgccga tgacgatgcg gtcgcaggct gcaaacgatc tctcgccgtg catcgacccg
     6181 ctgcggatcg gcaacccgct gccgccgccg gtcctgccga gcgtgaaggt ctcgcagagc
     6241 cggctcgccg agcggcaggc caaggcgaa
//
LOCUS       DASUFX010000429        15414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_347655,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000429 DASUFX010000000
VERSION     DASUFX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347655"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFV99_17150"
     CDS             complement(<1..267)
                     /locus_tag="VFV99_17150"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061098.1"
                     /translation="MLVEAGVLSEQGLRMALNEQRRWGGTLGRTLVDMKLLSEVELVR
                     VLSMQLALPTVDLDAIEIHSTVLDLVPGELAEQYSLVPFAQPMKF"
     gene            complement(294..2192)
                     /locus_tag="VFV99_17155"
     CDS             complement(294..2192)
                     /locus_tag="VFV99_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061099.1"
                     /translation="MRLSIGVCAVLLVGCADDVTAYFAVPGSTKGDDFYALPYPNDFW
                     RHDDGTLDLSQFPTNSVIATTVRDLAEQKLDGFGMSAALFARFSGPLDPLSLPSPAES
                     MKEGASVYLIDVDPDSPNRGTRYPIVATFHPEGTQTMGPNRLAVRPYPGFPLADGTTY
                     ALVLTNRMHSADGGDVNAQSDWSAVNGNGSASAPIMKARAVYAPLLDWLDDAGDDERG
                     DVVAAAVFTTQHAVNIIPAIRKGLFATPAPVARDIMVPQMGSAYGLFIGNYDAPNFQT
                     GDAPYITTGGEIVIGADGAAIVQRMESMRFQLSVPSIPVPPNGLPICIYQHGTGGDWT
                     SFVEDGTAERLAAEGIAVISTDQVLHGPRNPDGTSPEIAFFNFNNPPAARDNPLQGAA
                     DAWSQMRLALGMSFDDGSGTNRTIKFDPNRVFFFGHSQGGLTGPGFVAFEPTLKGAVL
                     SGTGGVFYESLLNKTEPVNFPDLIETLIRDNPVDEDNPTLALAQMFFERSDTINYAPF
                     MARIPQTAPDGTPLAPRNIFHTEGFTDHYSPNAAIEAFATALGGDLVMTADTKELEGV
                     TLRGRSIVPAPIKGNLDGATVVTAQYNQAGSSDGHFVVFDVGAARTQSSKFLGTLAAT
                     GQATVVSP"
     gene            complement(2222..2962)
                     /locus_tag="VFV99_17160"
     CDS             complement(2222..2962)
                     /locus_tag="VFV99_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061100.1"
                     /translation="MIRNVAFAFGVALTIAGAVAHAQPAGVDSKRRIVGILEVRVEGV
                     PKEIARQFQSDLEKQLDSREYWLAPQQRMREMMANSTKWTEGCVVGLCLNEVRAQTGA
                     EIVVLASITGSGTSFGYVVTLMRTDTGRMLAQEASRCEVCTVNEVLTSATLAAVKLLN
                     AAPDRLPDEAGEQSAALDVATSKLQVQKTAQERHNRSRGIALTLAGLVAAGAGATMYL
                     ALDRPAWALGTAAAGAGLAVGGVVTLTF"
     gene            complement(2959..3465)
                     /locus_tag="VFV99_17165"
     CDS             complement(2959..3465)
                     /locus_tag="VFV99_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061101.1"
                     /translation="MAEPSKEAAASAQGLYASYDAFLKQAIREYYDRGWTTRKGNFIA
                     LLIASGSTSMALAKDSVVDGSGTKKVAIGAGLAIALRVGLKYALGGPLGLVLTVAAGA
                     SMVAYFVRNQKDIVKKVGTYKTTIADSQKRYDEVQAGWRDGKYQITDRNLMIDGLMKQ
                     FITQVDEA"
     gene            3535..6315
                     /locus_tag="VFV99_17170"
     CDS             3535..6315
                     /locus_tag="VFV99_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061102.1"
                     /translation="MRNALALVLVCALGTAAHADLASGRDKLIAGDYKTAIAELAKVT
                     GKDQKAARILLARAQMATGDYAAAEGTLGPIAKDKDAQAIEARLVLDDLRRETGRIAD
                     ARKDLEALFKDKPDDRAVRTALAEVRHGQGNVVDAKTLFDMTIKEFDGQKLNLDDPLQ
                     LFQLAEAAKFTSQYELANDSYRAALKLNPKLTDIGVEWADLFSRKYASELAGQTLEEV
                     FKVNPNHPDAHAAMAGVIVETSYDLAAVRHHLDAALAVNPKNVRALRVRASIEIDRNE
                     WDAAQKTLGQILAINGQDLEAIAMKATIAWLRDDLKTYDAEKAKAFAINPAYAELYRI
                     VSRSAVREHRYVEAIELEKQAIKLKPDFFEAMAGAGLGYLRLGNEKEGLEWLDKAYKG
                     DGYNVRTVNTLNLFENQIPKDYSFHATKNFRIRYHNDEKPALSRYLEPTMERAFADMV
                     KRYGMTPKTPVTLELYPDKEAYAVRTVGLPDVAALGVCFGQVITAMSPMTGDLNWGMV
                     LWHELGHVFAIQLSNSRVPRWFTEGLSEYETLIARPEWRRENDADLYGAMANGTLPSI
                     GDLNSEFMQPDASAVVVAYFLSAVTIEWLAQTYGFPKLVDALKLYGKGQETPEVLKAI
                     TGKTIAQLDADFRKYLEIRLAPYAGTFKLPTRGFDDVTKLEIAADAAPKDAKARANVA
                     LGYYYAGDADKAGAAATKAIALDPKNPIARYILAEIAVHTGKAGDAKKMYTSLMVDGH
                     DSYDLRARLAQLAQEEQDAAEVEKQLCAAKKLDPERSYPYQALAELYKKQGKKAQYLS
                     ELEQYAFIEQMELAPLKELVDGYAQDKNWPKVRTYGEYATFISTQDPDVLNALGRAYL
                     ELGQPDRALYTYDTLLLVKPEPRRPALVHLGRARAFVALNKKADAKAAIAQAMKTEPE
                     NAEVLELKAKLK"
     gene            complement(6369..7118)
                     /locus_tag="VFV99_17175"
     CDS             complement(6369..7118)
                     /locus_tag="VFV99_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061103.1"
                     /translation="MSGEDKRKSDREPVTLFVEYEGADDLVGDYTDNLSSGGTFVATN
                     RELPVGTQITLVLGFPGLLEPISVQGTVRWTRARSEGELEEAGAGIEFEPGPARDQLA
                     AMIEKIRHGDAKTVSRIVKVLVVEDNRHVAQLINDGLKGAAKRDFGGGVQFQISNAED
                     GRQAIEALRNDTFNALIIDIYLPVVDGAKVIATARTELGLTKLPIIAVSAGGDSARRD
                     ALGAGANIFLDKPMRLRQVIETIQKLITTIG"
     gene            complement(7161..8693)
                     /locus_tag="VFV99_17180"
     CDS             complement(7161..8693)
                     /locus_tag="VFV99_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5061104.1"
                     /translation="MAGLSARILGGFTGLLRTLSGRIIIGFAVLTLTFAGVTTNIVVN
                     QSRLEGWIEQVNTGYLPLSQLAKELARRSEDVKTYLAEAFDKEASADSARIKLDTLRQ
                     RRAKALAETQKFFADLELDLDRTHTLRDDVDRLAMLIEAQKPHYDSVELALRQGEGLS
                     SQAVNEGLAPLRDGERQIASLASEISRRVSVSVQTYLGMLQKREQEARWITIGLGLVA
                     VVLGMLITTWVVITLRPLRRLREAARRVAAGEYGQSIEEKGPAEVVELAREFNSMGRA
                     IQEREREVVRSERLAAVGKMAAMITHEVRNPLSSISLNTELLQDELAKESTEAKDLLR
                     AIQREIDRLTALTEDHLAFARLPKPKPVPEAVNPLVGALAQFMREHLAAKQIKLSVEL
                     ADGDPIAFVDAAQVRQCLINLIRNAEEALAGKPSGTITLRTRQSGDRIHIDVEDNGPG
                     IPNTVLTRMFDPFFSTKENGSGLGLALTQQIVKDHGGDLAVQSTVGRGTTFTISVPAK
                     PA"
     gene            8727..9938
                     /locus_tag="VFV99_17185"
     CDS             8727..9938
                     /locus_tag="VFV99_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061105.1"
                     /translation="MTRALSLTCTALVLLGLSGSAFAGKPSIAVLGLEVVDQSGTPTP
                     ADTQAAKELSDGLRARAKAGTGPYQLAPGSDKELIDQKLLNNCDTEAPTCMSAIGNQL
                     GSDILMYGHFEKQGKAYQVTIRVLDVSRKSVLKSSSDMIPLGEASGAALQGWAKKIYA
                     KLTGESTGGTLVVKLANAERGSILIDGDQKGTITNGTGTVSGLGEGKVRVGVESEGFR
                     RWEKDITITSGSQTVPVELEKGGGEVIGPGPGPGPGPGPGPGPEGGSSKDKIIWGSVA
                     VGGLVVAAGGGYWLYHNKSIIDDVRADQCSHGWSNDGCVPTGSKTGFDIAASDSKGDS
                     ARTQTWVGGIAMGVGLGVAAIGIVKVIASTTAKNGEHAARGHRTRKERTFVVTPVVSK
                     DTNGATLRFDW"
     gene            complement(9921..12251)
                     /locus_tag="VFV99_17190"
     CDS             complement(9921..12251)
                     /locus_tag="VFV99_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX5061106.1"
                     /translation="MSADDDHPLVPRADVHLTDVPRAAGGLGALWATLRHLRRDSGIA
                     RGTKALLAMNQVGGFDCPGCAWPEPNAGERARFEFCENGAKALAEEATTARATPEVFA
                     AATVDEMRALSDFELGQLGRITHPLVLGPDRRYHAIEWDEAIAMIADELRAAGPAASA
                     FYTSGRTSNEAAFLYQLLGRMFGTNNFPDCSNMCHESSGNALLEVVGVDKGTVSLGDF
                     EHCDLIFVIGQNPGTNHPRMLTTLREAAKRGATIISINPLREVGLSRFSHPQHPLDLL
                     AGGVPLASEFVQVQIGGDQAFFLGVGKAVLEIDAAKAHETGETAVQTLVSESKTLPDL
                     MFLADKTDNFFAWRDHAIATPWSAIVECSGVDIETIRRVAAKYAEAKAVIACWAMGIT
                     QHKHAVRTIHEIVNLMLLRGNIGRPGAGLCPVRGHSNVQGDRTMGIDHHLRHPFLDRL
                     GAAFDFQPPRVAGFDTVGTIAAMERGEIRAFVMLGGNFLSAAPDTARTAFALDRCRLT
                     VSISTKVNRTHLYPGTRALILPCLARSELDGEQFVTVEDSMSMVHTSRGTLPPASDQL
                     RSEPAIIAALGEALLGERVPWREYAADYDKIRDKIAQVVLGFTSFNERVRDPNGFQLP
                     NTARDGSFANVGGRARFTVSPLPDLSLPPGRLRMMTMRSHDQYNTTIYGLDDRYRGIR
                     GERRVVFMNAADMVERGLVERQVVDLIGEWHDGERVAEAFIVVPYDLPRGNCATYFPE
                     ANVLVPLDSYADRSNTPTSKSVVIRVQPRPALPVEA"
     gene            12383..12964
                     /locus_tag="VFV99_17195"
     CDS             12383..12964
                     /locus_tag="VFV99_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061107.1"
                     /translation="MADPDELLKKAGGFFSKLGKQAKETAVTAGSQIKQTAKQVTGLG
                     RGTVKLELDQTKAAPGGSIHGRVVLALSEPVEAKKLVVTLRARQKMVTVKRSDAGRSV
                     GTSHADVYEFNLELGSAKKYESETLSFELTVPPDALDLRPQGPSSPLGEVARTIASAV
                     SPSAGPIEWQVITTLEIPWGRNLTHDVDIVVAR"
     gene            13129..15144
                     /locus_tag="VFV99_17200"
     CDS             13129..15144
                     /locus_tag="VFV99_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061108.1"
                     /translation="MSLCIASTAWAGKPLVDAGHFADESQLFLQLGNTDSGYWLSVQA
                     YAVGFVEKDRARIEVRQGGKQQFSYACQVGWDGGLAKVSCDGPQSGKLLKKAGPVEVD
                     LIQESDADDKEYLLRTFKVNAKNWDGKREWQIDGDDLLGAAYFAPTHAQTGEYATLRF
                     WVANGADFNAILRCTIDGKSVPDIDGTVESKNSLTADIIKGNSRTTWHWTQAAFYATR
                     FKITSVGKDPESVYVAEHPGSWQCQLRKGGITVREFSFKTNAKGDAEPHPMQQGKGAP
                     PLPPRVAMVDVKIPKDNGLDQRIKPDVIKKSRGFGLAWPQHDSVKVLLASLPPAYENA
                     NPPPPKKGPVKLVNGLEANPPKLVDESSTTVSVWRNGNGYFPRAKATIAGTTQAPSNM
                     YRLEWKQGAKLLATLKCSWQQSDNRKQDWNTVECEDNNLSLTAKGAIEANLIYTDDQQ
                     GVDYLVRTYKVTVAKYPSKGDPVWQVVPDDVLATAWAHQGGESVKIRFWLGMDNTSVE
                     LRCTVDGKKLPDIKMSGHADDNNIEATVYDNKANATKYVWQQAGGGGTDVQVGMPPKD
                     FKPSDGIVQYLGQYPGKWDCIVRQNGKQLREVLFTVNNKGSIDADPNQPGVYPSWVVP
                     IELRFGKDILDGRVRPDAMKKSRGFGLPWPKPPTTKFPAASGLPDPK"
     gene            complement(15179..>15414)
                     /locus_tag="VFV99_17205"
     CDS             complement(15179..>15414)
                     /locus_tag="VFV99_17205"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5061109.1"
                     /translation="DEAREKFPFYASRRIPEYWIVDPETREIEVYILRGDIYFAVAAN
                     RDGVIYAPRLQLELKVVDGPKLRIAWADGSADI"
BASE COUNT         2730 a         4959 c         5173 g         2552 t
ORIGIN      
        1 gaacttcatc ggttgcgcga acggcacgag gctgtattgc tcggctagct cgccgggcac
       61 gaggtcgagg acggtcgagt ggatctcgat cgcatcgaga tcgaccgttg gcagcgcgag
      121 ctgcatcgag agcacgcgta cgagctcgac ctcgctcaac agtttcatgt cgacgagcgt
      181 gcgcccgagc gtgccgcccc agcggcgctg ctcgttgagc gccatgcgca gaccctgttc
      241 gctgagcaca cccgcctcca ccaacatctc ccccaacttc ttacgcgcca tggttacgga
      301 gacactaccg tggcctgccc cgtcgcagcc agcgtgccga gaaacttgct cgactgcgtg
      361 cgggccgcac cgacgtcgaa tacgacgaaa tggccatcac tcgagccggc ttgattgtat
      421 tgcgccgtaa caaccgtcgc gccgtcgagg ttgcccttga tcggtgccgg cacgatgcta
      481 cggccgcgca gcgtcacgcc ttcgagctcc ttggtgtctg cggtcatgac gagatcgcca
      541 ccgagtgcgg tcgcgaacgc ctcgatcgct gcgttcggcg agtagtgatc cgtgaagcct
      601 tcggtgtgga agatgttgcg cggtgcgagc ggcgtgccgt cgggcgcggt ctgcgggatg
      661 cgcgccatga acggtgcgta attgatcgtg tccgagcgct cgaagaacat ctgcgcgagc
      721 gcgagcgttg gattgtcctc gtcgaccggg ttgtcgcgga tcagcgtctc gatcagatcg
      781 ggaaagttca ccggctcggt cttgttgagc agactctcgt agaacacgcc gccggtgccg
      841 gacagcacgg cgcctttgag cgtcggctcg aacgcgacaa agcccgggcc ggtgaggcca
      901 ccttgcgagt ggccgaagaa gaacacgcga ttcggatcga acttgatcgt gcggttcgtg
      961 ccgctgccgt cgtcgaagct catgcccagc gcgagccgca tctgcgacca cgcgtcggcc
     1021 gcgccctgca gtgggttgtc gcgtgcggcg ggcgggttgt tgaagttgaa gaacgcgatc
     1081 tcgggcgacg tgccatcggg attgcgcgga ccgtgcagca cttgatcggt cgagatcacc
     1141 gcgatgcctt cggcggcaag gcgctcggcg gtgccgtcct cgacgaacga tgtccagtcg
     1201 ccgccggtgc cgtgctggta gatgcagatc ggcaggccgt ttggcggcac cggaatgctc
     1261 ggcacgctca gctggaagcg catcgactcc atgcgctgga cgatcgccgc accgtcggcg
     1321 ccgatgacga tttcgccacc cgtcgtgatg tacggcgcgt cgccggtctg gaagttcggc
     1381 gcatcgtagt tgccgatgaa caggccgtac gcactgccca tctgcggcac catgatgtcg
     1441 cgcgcgaccg gcgccggcgt cgcgaacagc cccttgcgga tcgcagggat gatgttcacc
     1501 gcgtgctgcg tggtgaacac cgccgccgcg acgacatcgc cgcgctcgtc atcgcccgcg
     1561 tcgtcgagcc aatcgagcaa cggcgcgtaa accgcgcgcg ccttcatgat cggcgccgag
     1621 gccgagccgt taccgttcac tgccgaccag tcgctctgcg cgttgacgtc gccgccatct
     1681 gcgctgtgca tgcggttcgt cagcacgagc gcatacgtgg ttccgtctgc gagcggaaag
     1741 ccagggtagg ggcgcacggc gagtcggttc gggcccatcg tctgcgtgcc ctcgggatga
     1801 aacgtcgcca cgatcggata ccgcgtgccg cgattcggcg agtcgggatc gacgtcgatc
     1861 aagtagaccg acgcgccttc cttcatcgat tcggccggcg acggcaagct cagtggatcg
     1921 agaggccccg agaaccgcgc gaacagcgcc gcgctcatgc cgaagccgtc gagcttttgc
     1981 tccgcgaggt cgcgcacggt ggtcgcgatg acgctgttcg tcgggaactg cgaaagatcg
     2041 agcgtgccgt cgtcgtggcg ccagaagtcg ttcggatacg gcagcgcgta gaagtcgtcg
     2101 cctttcgtgc tgccgggcac ggcgaagtac gcggtgacgt catcggcgca accgacgagg
     2161 agcaccgcac agacgccaat ggatagcctc atggacgggc accctatcag aacgcccggg
     2221 gtcaaaacgt gagcgtgacc acaccaccga cggcgagccc ggcgccagcg gcggcagtac
     2281 cgagtgccca ggctggccgg tccagcgcca gatacatcgt cgcgcctgca ccggccgcga
     2341 cgagaccagc gagcgtcaac gcgattccgc ggctgcggtt atgccgctct tgcgcggtct
     2401 tctgtacctg caatttgctc gtcgcgacgt cgagcgcggc gctctgctcg cccgcctcgt
     2461 ccgggagccg atcgggtgcc gcgttgagca gcttcaccgc ggcgagcgtc gcgctggtca
     2521 gcacttcgtt gacggtacag acctcgcagc gactcgcttc ctgcgcgagc atgcgcccgg
     2581 tgtcggtgcg catcagcgtg actacgtagc caaagctcgt gcccgacccg gtgatcgatg
     2641 cgagcacgac gatctcggcg ccggtctggg cgcgcacctc gttgaggcac agcccgacaa
     2701 cgcacccctc ggtccatttc gtcgaattcg ccatcatctc gcgcatgcgt tgctgcggtg
     2761 cgagccagta ctcgcgactg tcgagctgtt tctctagatc gctctggaac tgacgtgcga
     2821 tctccttcgg cacaccttcg acgcgaacct cgaggatgcc gacgatgcgg cgcttgctgt
     2881 cgacaccggc tggctgcgcg tgagcgacag cgccagcgat cgtgagcgcg acgccgaacg
     2941 cgaaggcgac gttgcggatc acgcttcgtc gacttgggtg atgaactgct tcatcagccc
     3001 gtcgatcatg aggttgcggt cggtgatctg gtacttgccg tcccgccacc ccgcttgcac
     3061 ctcgtcgtac cgtttctgac tgtcggcgat cgtggtcttg taggtgccga ccttcttgac
     3121 gatgtccttc tggttgcgca cgaagtacgc gaccatcgat gcgccggccg cgaccgtgag
     3181 tacgaggccg agcgggccgc caagcgcata cttgaggccg acgcgcagtg cgatggccaa
     3241 ccctgcgccg atcgccacct tcttggtacc cgagccgtcg actaccgaat ctttcgcaag
     3301 tgccatcgac gtcgagcccg acgcgatgag cagcgcgatg aagttgccct tccgcgtcgt
     3361 ccaaccgcgg tcgtagtact cgcggatggc ctgcttcagg aacgcgtcgt acgaggcata
     3421 cagcccctgc gcgcttgcgg cagcttcttt agacggttcg gccacgtagg cagcttagca
     3481 gagatattcg ggttggtttg acaggggtgg tccggtcgca cagattcgag gggcatgcgg
     3541 aacgcattgg cgctggtact cgtttgcgct ctaggaactg ccgctcacgc ggacctcgcc
     3601 agtggtcgcg acaagctcat cgcgggtgat tacaagaccg cgattgccga gctcgcgaag
     3661 gtgaccggca aagatcagaa agccgcgcga atcctgctcg cccgcgccca gatggcgacg
     3721 ggtgattacg cggccgccga gggcacgctg gggccgatcg cgaaggacaa agacgcgcag
     3781 gcgatcgaag cgcgcctggt gctcgacgac ctgcgccggg agaccggccg cattgccgac
     3841 gcacgcaaag atctcgaagc gctgttcaag gacaagcccg acgaccgtgc cgtgcgaacc
     3901 gcgctcgccg aagtgcgcca tggccagggc aacgtcgtcg acgcgaagac gctgttcgac
     3961 atgacgatca aagagttcga cggccagaag ctgaacctcg atgatccgct gcagctgttc
     4021 cagctcgccg aggcggcgaa gttcacgtcg cagtacgagc tggcgaacga ctcgtatcga
     4081 gcagcgctga agctgaaccc caagctgacc gacatcggcg tcgaatgggc ggatctgttc
     4141 tcgcgcaagt acgcgagcga gctcgccggg cagacgctcg aagaggtgtt caaggtcaac
     4201 ccgaaccatc cggacgcgca cgcggcgatg gcgggcgtga tcgtcgagac ctcgtacgac
     4261 ctcgcggcgg ttcgtcatca cctcgacgcg gcgctcgcgg tgaatccgaa gaacgttcgc
     4321 gcactgcgcg tgcgggcgtc gatcgagatc gatcgcaacg agtgggacgc cgcgcagaag
     4381 acactcggcc agatcctcgc gatcaacggc caggacctcg aggcgatcgc gatgaaggcg
     4441 acgatcgcgt ggctgcgcga cgacctcaag acgtacgacg cggagaaggc gaaggcgttc
     4501 gcgatcaacc cggcgtacgc cgagctgtac cggatcgtgt cgcgctcggc ggtgcgcgag
     4561 catcgctacg tcgaggcgat cgagctcgag aagcaggcga tcaagctcaa gccagatttc
     4621 ttcgaggcga tggcgggcgc gggccttggc tacttgcgcc tgggcaacga gaaggaaggg
     4681 ctcgagtggc tcgacaaggc gtacaagggc gacggctaca acgtccgcac cgtcaacacg
     4741 ctcaacctgt tcgagaacca gatcccgaag gactactcgt tccacgcgac gaagaacttc
     4801 cgcattcgct atcacaacga cgaaaagcca gcgctgtcgc gttacctcga gccgacgatg
     4861 gagcgcgcgt tcgccgacat ggtgaagcgc tacggcatga cgccgaagac gccggtgacg
     4921 ctcgagctgt atcccgacaa agaagcgtac gcggtgcgta ccgtcggtct gcccgacgtc
     4981 gccgcgctcg gcgtgtgctt cggccaggtg atcacggcga tgtcgccgat gaccggtgat
     5041 ctcaactggg gcatggtgct gtggcacgag ctcggccacg tgttcgcgat ccagctgtcg
     5101 aacagccgcg tgccgcggtg gttcaccgaa gggctcagcg agtacgagac gctgatcgcg
     5161 cgtcccgagt ggcggcgcga gaacgacgcc gatctctacg gcgcgatggc gaacggcacg
     5221 ctgccgtcga tcggcgatct caacagcgaa ttcatgcagc ccgatgcgag tgccgtcgtc
     5281 gtcgcgtact tcttgtccgc ggtgacgatc gagtggctcg cgcagacgta tggctttccg
     5341 aagctcgtcg acgcgctcaa gctctacggc aaaggccagg agacgccgga ggtgctcaag
     5401 gcgatcacgg gcaagacgat cgcgcagctc gacgccgatt tccgcaagta cctcgagatc
     5461 cgcctcgcgc catacgccgg cacgttcaag ctgccaacgc gcggcttcga cgacgtgacg
     5521 aagctcgaga tcgccgcgga cgctgcgcca aaggatgcga aggcgcgtgc gaacgtcgcg
     5581 ctcggctact actacgcggg cgacgccgac aaggcgggcg cggcggcgac caaggcgatc
     5641 gcgctcgatc cgaagaatcc gatcgcgcgc tacatcctcg ccgagatcgc ggtccacacc
     5701 ggcaaggccg gcgacgcgaa gaagatgtac acgtcgctga tggtcgacgg ccacgacagc
     5761 tacgacctgc gggctcgtct cgcgcagctc gcgcaggaag agcaggacgc tgcggaggtc
     5821 gagaagcagc tgtgcgccgc gaagaagctc gaccccgagc gcagctaccc gtaccaagcg
     5881 ctcgccgagc tgtacaagaa gcagggcaag aaggcgcagt acctcagcga gctcgagcag
     5941 tacgcgttca tcgagcagat ggagctcgcg ccgctcaagg agctcgtcga cgggtacgca
     6001 caggacaaga actggccgaa ggtccgcacc tacggcgagt acgcgacgtt catcagcacg
     6061 caggatccgg acgtcttgaa cgcgctcggt cgcgcctacc tcgagctcgg ccagcccgac
     6121 cgcgcgctct acacgtacga cacgctcctg ctcgtgaagc cggagccgcg gcggcccgcg
     6181 ctcgttcacc tcggtcgcgc gcgcgcgttc gtcgcgctga acaagaaggc ggatgcgaag
     6241 gccgccatcg cgcaggcgat gaagaccgag cccgagaacg ccgaggtact cgagctcaaa
     6301 gcgaagctca agtaagccgg agatccgagc ttctcgccga agttcgaagc ccgtagtccg
     6361 aagcccgatc agccgatcgt cgtgatgagc ttctggatcg tctcgatcac ctgacgaagc
     6421 cgcatcggct tgtcgaggaa gatgttcgcg ccggctccga gcgcgtctct gcgcgccgaa
     6481 tcgccgccgg ccgacaccgc gatgatcgga agcttggtca atccgagctc ggtgcgtgcg
     6541 gtcgcgatca ccttcgcgcc atcgacgacc ggcagataga tatcgatgat cagcgcattg
     6601 aacgtgtcgt tgcgcagcgc ttcgatcgcc tgcctgccgt cctccgcgtt gctgatctgg
     6661 aactgcacgc caccgccgaa gtcgcgcttc gcggcgccct tgagcccgtc gttgatcagc
     6721 tgcgcgacgt gacggttgtc ctcgacgacg agcaccttga cgatgcgcga gaccgtcttc
     6781 gcatcgccgt ggcggatctt ctcgatcatc gccgcgagct ggtcgcgcgc aggaccgggc
     6841 tcgaactcga tgccggcgcc ggcttcttcg agctcgcctt cgctgcgggc gcgcgtccat
     6901 cgcacggtac cctggacgga aatcggctct aggaggcccg ggaagccgag cacgagcgtg
     6961 atctgggtgc cgaccggcag ctcgcgattc gtcgcgacga acgtgccgcc cgacgacaag
     7021 ttgtcggtgt agtcgccgac caggtcatcg gcgccctcgt attcgacaaa caaggtgacc
     7081 ggctcgcggt cgctcttgcg cttgtcttcc ccggacatca gctagcaatg atccatcgct
     7141 agcttcgcga gcgcaatggt tcacgcgggc ttcgcgggca cactgatggt gaaggtcgtg
     7201 ccgcgtccca ctgtactttg gacagcgaga tcaccgccgt ggtccttgac gatctgctgc
     7261 gtgagcgcga gaccgaggcc gctgccgttt tctttcgtcg agaagaatgg gtcgaacatg
     7321 cgcgtcagga cggtgttcgg gatgccggga ccgttatcct cgacgtcgat gtggatgcga
     7381 tcgccgctct gacgtgtgcg gagcgtgatc gtgccgctcg gtttgccagc gagcgcttcc
     7441 tcggcgttgc ggatgaggtt gatcaggcac tgccgcacct gtgcggcgtc gacgaacgcg
     7501 atcggatcgc catcggcgag ctccacggag agcttgatct gctttgccgc caggtgctcg
     7561 cgcatgaact gggcgagtgc accgacgagt gggttgaccg cctcgggaac cggctttggc
     7621 ttcggcaagc gcgcgaacgc gaggtggtcc tcggtgagcg cggtgaggcg atcgatctcg
     7681 cgctggatcg cgcgcaataa gtccttcgct tcggtggatt ccttcgcgag ctcgtcctgc
     7741 agcagctcgg tgttgaggct gatcgacgac agcgggttac gcacctcgtg cgtgatcatc
     7801 gcggccatct tgcccacggc ggcgaggcgc tccgagcgca ccacctcgcg ctcgcgctcc
     7861 tggatcgcgc ggcccatcga attgaactcg cgcgcgagct cgacgacctc ggcaggtccc
     7921 ttctcttcga tgctctggcc atactcgccc gccgccacgc gtcgcgccgc ttcacgcaac
     7981 cggcgcagcg ggcgcagcgt gatcacgacc cacgtcgtga tcagcatgcc gagcacgacg
     8041 gcgacgaggc cgagcccgat cgtgatccag cgcgcctctt gctcgcgctt ctgcagcatg
     8101 ccgagatagg tctggacgct gaccgacacg cgccgcgaaa tctcgctggc gaggcttgcg
     8161 atctgacgtt cgccgtcgcg cagcggtgcg agaccctcgt tgaccgcctg gctgctcagc
     8221 ccctcgccct ggcgcagcgc gagctcgacg gagtcgtagt gtggcttctg cgcctcgatc
     8281 agcatcgcca ggcggtcgac gtcgtcccgc agcgtgtgcg tgcgatcgag atcgagctcg
     8341 aggtcggcga agaacttctg cgtctcggcg agtgccttgg cgcggcgctg gcggagcgta
     8401 tcgagcttga tgcgcgcgct atctgcgctg gcctccttgt cgaaggcctc ggccaggtac
     8461 gtcttgacgt cctccgagcg ccgggccagc tccttggcaa gctgggagag tggcaaatat
     8521 ccggtgttga cctgctcgat ccacccctcg aggcggctct gattgacgac gatgttggta
     8581 gttacgccgg caaaggtcag ggttagtacg gcgaacccga taatgatgcg ccccgaaagc
     8641 gtgcgtaaaa gcccggtgaa cccgccgagg attcgcgctg ataacccagc cacgattcat
     8701 gtatactacc tcctctgagg gagcccatga cccgtgcact ttcactgact tgtaccgcgc
     8761 tggtccttct cggtctcagt gggtcagcct tcgcgggcaa gccgtcgatc gctgtcctcg
     8821 gtctcgaggt cgtcgatcaa agcggcacac caacaccggc ggacacgcaa gccgccaaag
     8881 agctcagcga cggcctgcgt gcacgcgcga aagcgggcac cggtccgtac cagctcgcgc
     8941 cgggcagcga caaagagctg atcgaccaga agctgctcaa caactgcgac accgaggcgc
     9001 cgacgtgcat gtcggcgatc ggcaaccagc tcggctcgga catcctgatg tacgggcact
     9061 tcgagaagca gggcaaggcg taccaggtca cgattcgcgt gctcgacgtc agccgcaagt
     9121 cggtgctcaa atcgtcgagc gacatgattc cgcttggcga ggccagcggc gctgcactgc
     9181 agggctgggc gaagaagatc tacgcgaagc tcacgggcga gagcacgggc ggcacgctcg
     9241 tcgtcaagct cgcgaacgcc gagcgcggct cgatcctgat cgacggcgat cagaagggca
     9301 cgatcacgaa cggcaccggc acggtgagcg gcctcggtga aggcaaggtc cgcgtcggcg
     9361 tcgagtcgga aggcttccgc cgctgggaga aggacatcac gatcacgagt ggctcgcaga
     9421 cggtgcccgt cgagctcgag aagggcggcg gcgaggtgat cggtcctggc cccggtcctg
     9481 gtccgggtcc tggccccggt cccggccccg agggcggcag cagcaaggac aagatcatct
     9541 ggggctcggt cgccgtcggc ggcctggtcg tcgccgcggg cggcggctac tggctctatc
     9601 acaacaagtc gatcatcgac gacgtgcgcg ccgaccagtg ctcgcacggc tggtcgaatg
     9661 acggctgcgt gccgacgggc tcgaagacgg gctttgacat cgccgcatcg gacagcaagg
     9721 gcgattccgc gcggacccag acctgggtcg gcggcattgc gatgggtgtc ggcctcggtg
     9781 tcgctgcgat cggcatcgtc aaggtgatcg cctcgacgac cgcgaagaat ggcgagcacg
     9841 ccgcgcgcgg ccatcgcacc cgcaaggaac gcacgttcgt ggtgaccccg gtcgtgtcga
     9901 aggacacgaa cggcgcgacg ctacgcttcg actggtaacg ccgggcgagg ctgcacgcga
     9961 atcacgaccg acttcgacgt cggcgtgttg ctgcgatcgg cgtagctgtc gagcggcacg
    10021 agcacgttcg cttcggggaa gtacgtggcg cagttgccgc gcggcaggtc gtacggcacg
    10081 acgatgaacg cctcggcgac gcgctcgccg tcatgccact cgccgataag gtcgacgacc
    10141 tggcgctcga ccaggccgcg ctcgaccatg tcggcggcgt tcatgaacac gacgcggcgc
    10201 tcgccgcgaa tgccgcggta acgatcgtcg aggccgtaga tcgtcgtgtt gtactgatcg
    10261 tggctgcgca tcgtcatcat gcgcaagcgg cccggcggca gcgacaggtc gggcagcggc
    10321 gacaccgtga agcgggcgcg gccgccgacg ttcgcgaacg agccatcgcg cgcagtgttc
    10381 ggcagctgga agccgttcgg gtcgcgcacg cgttcgttga agctcgtgaa gccgagcacg
    10441 acttgcgcga tcttgtcgcg gatcttgtcg tagtcggcgg cgtactcgcg ccacgggacg
    10501 cgttcgccga gcaatgcctc gccgagcgcc gcgatgatcg caggctcgct gcgcagctgg
    10561 tcgcttgccg gcggcagcgt gccgcgcgac gtgtgcacca tgctcatcga atcctcgacg
    10621 gtgacgaact gttcgccatc gagctcgctg cgtgcgaggc agggcaggat cagcgcacgc
    10681 gtgccgggat agaggtgcgt gcggttgacc ttcgtcgaga tgctgacggt gaggcgacag
    10741 cggtcgagcg cgaacgccgt ccgtgcggtg tcgggtgccg cggacaggaa gttgccaccg
    10801 agcatcacga acgcgcggat ctcgccgcgc tccatcgctg cgatcgtgcc gaccgtgtcg
    10861 aagccggcga cacgcggtgg ctggaagtcg aatgcggcgc cgaggcgatc gaggaacgga
    10921 tggcgcaggt ggtgatcgat gcccatcgtg cgatcgccct gcacgttgga gtgaccgcgc
    10981 accgggcaca ggccggcgcc cgggcggccg atgttgccgc gcaggagcat caggttcacg
    11041 atctcgtgga tcgtgcgcac cgcgtgcttg tgctgcgtga tgcccatcgc ccagcacgcg
    11101 atcaccgctt tcgcttcggc gtacttcgca gcgacccggc ggatcgtttc gatgtcgacg
    11161 ccggaacact cgacgatcgc cgaccacggc gtcgcgatcg cgtgatcgcg ccacgcgaag
    11221 aagttgtcgg tcttgtcggc gaggaacatc aggtcgggca gcgtcttgct ctcgctgacg
    11281 agcgtctgca ccgcggtctc gccggtctcg tgcgctttcg ccgcgtcgat ctcgagcacg
    11341 gccttgccga cgccgaggaa gaacgcttgg tcgccgccga tctgcacttg cacgaactcg
    11401 ctcgcgagcg gcacgccacc ggcgagcaga tcgagtggat gctgcggatg cgagaaccgc
    11461 gacaggccga cttcgcgcag cgggttgatg ctgatgatcg tcgcgccgcg tttcgctgct
    11521 tcgcgcagcg tcgtcagcat gcgcggatgg ttcgtgcccg ggttctgacc gatcacgaag
    11581 atcaggtcgc aatgctcgaa gtcgccgagg gacaccgtgc ccttgtcgac accgacgact
    11641 tcgagcagcg cgttgccgct cgactcgtgg cacatgttcg aacaatcggg aaaattgttc
    11701 gtgccgaaca tgcggccgag cagctgatac agaaacgccg cttcgttcga cgtgcggccc
    11761 gacgtgtaga acgccgatgc ggccgggccg gcggcgcgca gctcgtcggc gatcatcgcg
    11821 atcgcttcgt cccattcgat cgcgtgatag cggcgatccg gcccgagcac gagcggatgc
    11881 gtgatccggc cgagctggcc gagctcgaag tcggagagcg cgcgcatctc gtcgacggtc
    11941 gccgccgcaa acacctcggg cgtggcgcgc gcggtcgttg cttcctcggc cagcgccttc
    12001 gcgccgttct cgcagaactc gaagcgcgct cgctcgccgg cgttcggctc cggccacgcg
    12061 cagcccgggc agtcgaagcc gccgacctgg ttcatcgcga gcagcgcctt ggtaccgcgc
    12121 gcgatgccgg aatcgcggcg taagtgcctg agcgtcgccc acaacgcgcc gagcccgccg
    12181 gccgcccgag gtacatcggt cagatgcacg tcggcgcgcg ggaccagagg atggtcgtcg
    12241 tcggcagaca cgcggcattg cgtaccacgg gcccgatgga tggcgcgcct tatcggcggt
    12301 tacacgcggt ggccgtgtgt tgctgggacg cagcgcgtcg gttttccgcg atggcgcagt
    12361 gagccatggt atggactccg gcatggcgga ccccgacgag ctcctcaaga aagcaggcgg
    12421 tttcttctcc aagctgggca agcaggccaa ggagaccgcg gtgaccgcgg gttcccagat
    12481 caagcagact gccaagcagg tcaccggcct cggccgcggc acggtcaagc tcgagctcga
    12541 ccagaccaag gcggctcccg gaggctcgat ccacggccgc gtcgtgctcg cgctcagcga
    12601 gccggtcgag gcgaagaagc tggtcgtgac gctgcgcgcg cgccagaaga tggtgaccgt
    12661 gaagcggagc gacgccggcc gcagtgtcgg cacgtcgcac gcagacgtct acgagttcaa
    12721 tctcgagctc ggcagcgcca agaagtacga gagcgagacg ctatcgttcg agctgacggt
    12781 cccgcccgac gcactcgacc tgcggccgca aggcccatcg tcgccgctcg gtgaggtcgc
    12841 gcgcacgatc gcgtcggcgg tgtcgccgtc ggcggggccg atcgagtggc aagtgatcac
    12901 gacgctcgag attccgtggg gccgcaacct cacgcacgac gtcgacatcg tcgtcgcgcg
    12961 ctagccgacg caatatcttc gccgcgctag tcgacgcaat atcttcgtcg cgcgctggtc
    13021 gacgcaaccg aacgcggcgc agtcgatacc gccgccctgc gctagccgac gcggttgcgc
    13081 ctccggtacg ttggcgccgg aggtttcatg cgcacattgg gtgttctgtt gtctttgtgc
    13141 attgcgagca cggcgtgggc gggcaagccg cttgtcgacg ccggccattt tgccgacgag
    13201 tcgcagttgt tcctgcagct cggcaatacg gatagcggtt actggctcag cgtccaggcc
    13261 tatgcggtcg gcttcgtcga gaaggatcgc gcgcggatcg aggtgcggca gggaggcaag
    13321 cagcagttct cgtacgcgtg tcaggtaggc tgggacggcg gtctcgccaa ggtctcgtgc
    13381 gacggtccgc agagcggcaa gctgctcaag aaggccggcc cggtcgaagt ggatctgatc
    13441 caggagagcg acgccgacga caaggaatac ttgctgcgca cgttcaaggt gaacgcgaag
    13501 aactgggacg gtaagcgcga gtggcagatc gacggcgatg atctactcgg cgccgcgtac
    13561 ttcgcaccga cgcacgcgca gaccggcgag tacgcgacgc tgcggttctg ggtcgcgaac
    13621 ggggccgact tcaatgcgat cttgcgttgc acgatcgatg gcaagtcggt gcccgacatc
    13681 gacggcactg tcgagagcaa gaactcgctc accgcggaca tcatcaaggg caacagccgc
    13741 acgacgtggc actggaccca ggcagcgttc tatgcgacgc ggttcaagat cacgtcggtc
    13801 ggcaaggatc ccgagagtgt ctacgtcgcc gagcatccgg ggtcgtggca gtgccagcta
    13861 cgcaaaggcg gcatcacggt ccgcgagttc tcgttcaaga cgaacgcgaa gggcgatgcg
    13921 gagccacatc cgatgcagca gggcaagggc gcaccgccgc taccgccgcg cgtcgcgatg
    13981 gtcgacgtca agatcccgaa ggacaacggg ctcgatcagc gcatcaagcc cgacgtgatc
    14041 aagaagagcc gcggcttcgg gctcgcgtgg ccgcaacatg acagcgtgaa ggtgctcctc
    14101 gcgtcgctgc cgccggcgta cgagaacgcg aatccgccgc cgccgaagaa gggcccggtc
    14161 aagctcgtga acggcctcga ggcgaatccg ccgaagctcg tcgacgagtc gtcgacgacg
    14221 gtaagcgtct ggcgcaacgg caacggctac ttccctcgtg cgaaagcgac gatcgccggc
    14281 accacgcagg cgccgtcgaa catgtaccgc ctcgaatgga agcagggcgc caagcttctc
    14341 gcgacactca agtgctcgtg gcagcagtcc gacaatcgca agcaggattg gaacactgtc
    14401 gagtgcgagg acaacaacct ctcgctcacc gcgaaaggcg cgatcgaagc gaacctcatc
    14461 tataccgacg accagcaagg cgtcgattac ctcgtgcgga cctacaaggt caccgtcgcg
    14521 aagtatccga gcaagggtga tccggtctgg caggtcgtgc ccgacgacgt gctcgccacc
    14581 gcatgggcgc atcagggcgg cgagagcgtg aagattcggt tctggctcgg catggacaac
    14641 acgagcgtcg agctccgctg caccgtcgac ggcaagaagc tcccggacat caagatgtcg
    14701 ggccatgcgg acgataacaa catcgaggcc accgtctacg acaacaaggc caacgcgacg
    14761 aagtacgtct ggcagcaggc cggcggcggt ggcaccgatg tccaggtcgg catgccgccg
    14821 aaggacttca agccgagcga cggcatcgtt cagtaccttg gccagtaccc gggcaagtgg
    14881 gactgcatcg tgcgccagaa cggcaagcag ctgcgcgagg tgctgttcac cgtgaacaac
    14941 aagggcagca tcgacgcgga tccgaatcag ccgggcgtgt atccgtcgtg ggtcgtgccg
    15001 atcgagctgc gcttcggcaa ggacatcctc gacgggcgtg tccgtccgga cgcgatgaag
    15061 aagagccgcg gctttggtct gccgtggccg aagccgccga cgacgaagtt cccggcggcg
    15121 agcggtctgc cggatccgaa gtagcgaccg tcgttatcgc gccgatgtgc agcgccgctc
    15181 aaatatcggc gctgccgtcg gcccacgcga tgcggagctt cggtccgtcg acgactttta
    15241 gctcgagctg taggcgcggc gcgtagatga caccgtcgcg attcgcggcc acggcgaagt
    15301 agatatcgcc gcgcaggatg tagacctcga tctcgcgcgt ctccggatcg acgatccagt
    15361 actcgggaat ccgtcgggat gcgtagaacg ggaacttctc tcgcgcctcg tcgt
//
LOCUS       DASUFX010000430        21253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_69049,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000430 DASUFX010000000
VERSION     DASUFX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69049"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFV99_17210"
     CDS             <1..290
                     /locus_tag="VFV99_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5061110.1"
                     /translation="LRGDQIRIHILIRGRIGEGWKDVDEYLRVPTGTTLGKLVEVADA
                     AGVPLSEALANSPHLTDTLMLNGERCPLEANRERELAEGDEIYLLAPLAGG"
     gene            complement(287..1852)
                     /locus_tag="VFV99_17215"
     CDS             complement(287..1852)
                     /locus_tag="VFV99_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5061111.1"
                     /translation="MRWLSRVITVVVFVGVVLLVAMWIRSKMPATSVGDTFKTCARFR
                     DGSRLAVGSYVLIAGVRVGEVTGLTLEGPLARIDMTLQNDINVPVDSWVTKRAYSPFG
                     DSYVEIIPTGGDEGAPTAQRLQSGQCLTRVLEGTSTDRMLRAIDDVMPRVTRGLDRLH
                     EVALYGRKWALGTLEDKMLDADHWLDEGNVERPIERAEQALARLEAGATSAANTMAGS
                     RPQVDKTLDRIQGGIDSARKQMAELTISLHDGMASVRSSMDGLDKPAADYGELMSRID
                     QGRGSGAQGTLGRLINDPHLADSIEEGTDTLREGTSSFSRFKSWLGLRAEFNVFAQQP
                     RFYVTAEVRARTDKFYLVELERGPLGGLPDDQISDMVGVPDYYRYQEIHDRLRYTVQF
                     GKQFGNSFDVRAGIKESTFGIGADVLMQNGRLKFSADMFGSYQKVPRVKLAGALAVFR
                     SIYLIAGVDDALNKPGYLQIRKGNPPGSDVPIQFDQVRFGRDFFLGAELHFDDADLSM
                     LLRVYGALLVGLL"
     gene            complement(1878..2912)
                     /locus_tag="VFV99_17220"
     CDS             complement(1878..2912)
                     /locus_tag="VFV99_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061112.1"
                     /translation="MRVATSVALATLLFGCGGGSGGGGGGDDDVNDPDGGQHDAPPFA
                     SGTGPYFSVPMFFNQDVSGVQKASYSDATIAALRAAGGWGNGDTFQIDFSIDVLNAPS
                     GTPMRTFTKTGDFYSPDCDDVAMPVPVGGNVEGETGYECTGDGDCHLLVNDGDGGKLY
                     EMWRANIVGSTFYGGCLAVWNTRMAYTDTLRGDQCTSADAAGFPISPLLFTADEVAAG
                     EIKHAIRFILPNNRVKRGFVRPATHGTNTTGGTNAPAYGVHFRLRADYPVDSLPSEGA
                     KVVARALQKYGMYHADGGNIALTAQDDRHTTAKWTNLLDPQDLADLKVEDFDVIDHGS
                     MITLTLDCMR"
     gene            complement(2981..3937)
                     /locus_tag="VFV99_17225"
     CDS             complement(2981..3937)
                     /locus_tag="VFV99_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061113.1"
                     /translation="MIAPLCVVTACATDAPGGDDTGSGPFFERSMFWNQDVSNVPKAA
                     NSDEWIAAIHAAGGWGNQDHLRVDFSFDVLHADASAPQTFTPTDQFQVPDCDHIPVPV
                     PAIGNVEGETSYKCSAGGDCHLIVLDDKNRKLYEMWKASISTMFSGGCLAVWDLTRKY
                     DETLRGDQCTSADGAGLPIAPLLFNADEVASGSIEHAIRFVLPNDRVTQGFVRPATHA
                     AYTSGGLELPFYGVHLRLRADYPVERLTTTGAQVVARALQKYGMYHADGGEDTLTAQS
                     DKYTTAKWDGLLGPQDLYPLEVSDFEVVDHGEGIPITNDCQR"
     gene            complement(4006..5871)
                     /locus_tag="VFV99_17230"
     CDS             complement(4006..5871)
                     /locus_tag="VFV99_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061114.1"
                     /translation="MVCLLVLALSFGCKKKPTIAELTKADGPVERQENQGPWGGANIG
                     QKFHIGDAARTADGAAELKLVGVQLIKMEKYTVLRFGGGKDGSAKITVELGAIVLKGG
                     GNYGLDVGDVKLGNDGEVRIAAKGQGKSTIELLVGNAQIDKGGQTLDLEIGKVLDLDL
                     TIGPIKLETPIDAGVDAAGDGGTDAGVIVSEDGTIEVTGKKAEILQPGQTKWAPLPAG
                     AGQLVKGAKVRLGNGTTAKLIANGVTLSMAGGSRTAIADDLHFGLELGVATASVEAQT
                     EGKVGVPGGTVGIKGTEKGPGKARLDVNAKGEAKIAVLDGTAKLTGSSGADLEMKTGE
                     SASMQKAGAIHQTAKIPDYYDMRVMIGETPTFTIHDPKGATALQFAFNGKCNGGGTIE
                     MDNDPRFRTARISGGRESANIIAEAGAWSYRLVCSGGGAGGSGRIIVRKDSGTRPLPK
                     DPPVNPIDADGRTYRISYQSLIPNVKVKAPGGPGKLKLHLATSGTEETFDSEKPTFTL
                     DGKKLKEATYTYWVERDGVKQDKASTLIINFDQTAPQVYIEQPQNGKPFGAEVDVKGA
                     VLPGWSAKVDAVEIPIDKNTRRFTAKVTPTTGPQALAIRLSHPQRGVHFYLRRGK"
     gene            complement(5906..7525)
                     /locus_tag="VFV99_17235"
     CDS             complement(5906..7525)
                     /locus_tag="VFV99_17235"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5061115.1"
                     /translation="MLLVPLTVSAQEAEISAGTSTDQSVAPAQDIVYGAMPGGLHAPT
                     AETLPKGAVEVSTLMGLGRRTGLLGPDHKFNRGVGDLAIAFGATPYLSIGISLDGRYD
                     RHWGTLGDPMLGDPTTVPAAPGGKNEDGYVGDPHLIVRASKNTGSLIAGGQLGIWVPG
                     KDAPSVAGSAISIDVRALVSLPAGPGLLSFNGGFRLDNSANSVDDPMKLSLPDRVSLG
                     VSDYHALFGGAQLRIPAGKAWVSVEGSIDAFVGGPPDPAAGEVKRASLERGKLIMRGG
                     ITGGFHITDQYSAVAFLEAAKVPGINDSQVDNANIPLVPYEPIITAGIGFEARFGGPK
                     AVPPSFTERECARHTPPDCPDVKADILTEVTGTVVDNGGKPVVGAKVTLTLKNAQVNP
                     VVTDEKGTYVFKGVLIGHSMGGKPTIEETAAEVGVEVSNMKPGKSTITQIAEGSNTVP
                     PIALEPVLPPGQLRGVVRSLPGGKAVGGATVTVAGSEAKATTGDDGTFTIDLAPGQYK
                     IKVSKAGLKDQELDVTIDPNGVAIKNIDLQK"
     gene            complement(7638..8873)
                     /locus_tag="VFV99_17240"
     CDS             complement(7638..8873)
                     /locus_tag="VFV99_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5061116.1"
                     /translation="MWVIAKREFYERVRSKWFVVMTILWPILMVGMMVVPALIGNQGT
                     EGAKVTIIDKSGRLAEQMQYQLGVVLKWNTTVAPPDTDEKTLLAKIKSDEINGYVVIP
                     KDALDGGEIVYNGDNASNQTVSMGFGQLAQRMVITERAKRAGLSEVDIALLKLPVNVS
                     VRHTTGEEQGTAGIFAFLVGYMIAFFIYILITLYGINVMRSVVTEKTSRVVELLVAAT
                     KPRAMMSGKILGVGFAGLLQIAIWFVIGYVVISQRETLLGLFGAKPDATSMMPSLTPI
                     QLAITLACFVLGYLFYSALYAAVGAMVSSEQDSQQAQMPVTFLLVIGIIAITAVTNDP
                     RGQAAQIMTMVPFWSPMLMPLRYFLGGASLAQVGLSLAILAAATVLVARAAAKIYRVG
                     ILMYGKRPSMRELVRWLRY"
     gene            complement(8875..9858)
                     /locus_tag="VFV99_17245"
     CDS             complement(8875..9858)
                     /locus_tag="VFV99_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5061117.1"
                     /translation="MTKPALTVTGLVKKYGNHVAVDGISFEVPRGSVFGILGPNGAGK
                     STTLRMINDIIAPDAGTITILDGLAPGHAAAKQIGYLPEERGLYPKMNVIDMIVFMGE
                     LRGLGHREAKVRAHKWLDRLGLADWGKNKVQDLSKGMAQKVQFATALIHEPALVILDE
                     PWSGLDPINAEVLVDVVEEIKSSGRTVMFSTHQMESAEKVCDQVCIIARGRKVLDGRL
                     KDIQRASVAEGVIALAFTDDAAKERAEPLLADEKLVIERKPPRPGEHADCEVVLADGI
                     KPDHFLKTLLNHDIGLRLFELVVPTLHQIFVQKVGAEAAVAARRDEEGAVA"
     gene            complement(9855..10847)
                     /locus_tag="VFV99_17250"
     CDS             complement(9855..10847)
                     /locus_tag="VFV99_17250"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5061118.1"
                     /translation="MTSGPRHLVSIADLDAAEIVRILDHGDRYWSLADEPIKKLTSLR
                     GRTVINLFYESSTRTRTSFELAAKRLSAEAINIAASSSSTTKGETLLDTGRNLQAMHP
                     DAIVVRHSFGGAAYLLAKTAKCAVVNAGDGQHEHPTQALLDAATIRRHKGELAGLEVA
                     IVGDIQHSRVARSNILCLSKLGARVRLVAPWTLIPRGIELIGGELTRERTRICARLED
                     GLDGADVVMMLRVQHERFEGEAPRFPNTRELSRTFGLSERTLAYAKPDAIVMHPGPIN
                     RGVEVSPQVADGPRAVILQQVSWGVAIRMAVLERAILGDAAGATAGAAGAAGAA"
     gene            10886..11749
                     /locus_tag="VFV99_17255"
     CDS             10886..11749
                     /locus_tag="VFV99_17255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061119.1"
                     /translation="MSGAKVSRIGSIIGDRFRLDELIGRGAMAEVYRALDLETQAFVA
                     VKILRHQHVTDTVSIARFAREADVQARLRHRNVAALLASGVTKDKHEPYLAVELLRGR
                     SLRSVIKSEGHVVPRRAASYTWQALQGLHAVHQAGVLHRDLKPANIMLEPSPGPIERV
                     VLIDFGFATFEGSARLTLQGTVVGSLTYIAPERLRAEEPDQRADIYAVGVVLFELLTG
                     QPPFAAEEDFDLISMHLHDPPPSLAHVVPSARALDPVLARALAKHPADRYADAQQMAD
                     DIEHAAKQLTE"
     gene            complement(11739..13664)
                     /locus_tag="VFV99_17260"
     CDS             complement(11739..13664)
                     /locus_tag="VFV99_17260"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEX5061120.1"
                     /translation="MEGSPRVALFTCIVALAGCGNAPRNSGDDTSPDALNNCAPSGAH
                     RCEGASYQTCYGGQWQTAIDCPALCSDSLGCVECTPGQNFCKDGNVWVCDDAGKPGCE
                     VSVCGGANTCVGGACVDACADAAASKSYIGCEYWAVDLDNAVEVWGGSSGTCAQGSPM
                     TLNVCYTMMGGGLYLGQCDPQNDSMAPHTCPAGTTCLPKLVCASDAQHGPFAVVVSNP
                     QAKDATVTVTGPGGQTITKVVTAGQVTPILMQANNAIPDQSVDGTGKFKKAYRITSDL
                     PVVAYQFNPLDNSNVFSNDASLLIPRATMDTDYYGMSWVTLNRRPNRDPYYGYLTIVA
                     PQDGTQIAVTPKTAVVASQTQATLVPGATVLFTLDAFEVLQLEAAVGTGTAPSGDLTG
                     THITSPNMMPFGVYGGHEATAFGETTAPDSMHPNGPGFADHLEEMLFPSTTWGMTFAI
                     ARSKQRSNEPDYLRILAQKPGTAVTFTPAPSASVYGNCSALAAGEFCDVKIQGDTEIS
                     ATQPILVGHFLESATWWNNGQNSFVGTGDPSMAIAVPSEQFRKEYTVLVPAQYTANYI
                     SIAAAATGGVQLDGAPVTMTAFPGGGTHRAARVTVPAGQHKITCADGCGITVYGYSDG
                     VSYMFAGGLDLKPIVIL"
     gene            complement(13704..15635)
                     /locus_tag="VFV99_17265"
     CDS             complement(13704..15635)
                     /locus_tag="VFV99_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061121.1"
                     /translation="MRDPRHVVLVALSLAACGSDGSSIPDQCNPMGGQGCLLPWPSAA
                     YEVEDATTATGRRVQVPIEAMPKNIDDVSIDPAWLDRWDGFSPTGPMLAAFPTGVSNT
                     NLPSWKNPDESLAADSPIVLLNMDTGERAPFFAEVDQNTADIEKRNLIIRPLARLAPG
                     AHYAVAIRNSVKAADGSDLPIPEAFAKALSGSGYGHSRYSKERYEKIFAALATAGVDK
                     SELVLAWDYYTASDEFLQRDLTTMRDAAIPAMGQNGANLTFTATQQAPRTGVLKSFTG
                     TFKSPDFLTNGEEDSSVMRRDANNLPQMMGLRDARFAAIVPECVTTQPLPRPTIIFGH
                     GLFGSAEGYLDDNFVVNLAQDYCLVILAGDFIGLTDRQLSLAPLAVNDMNKGKAITEK
                     LAQSVIDFISLETITRGQLGQHPEFQYNGASVIDPNKTFYVGGSLGGIMGNTFMAYDP
                     NITKGVLAVPGGVWSLMFERSNAWHLLMGAAQGAYEDPEVYQLNLAFLGMGMEPYDPI
                     TTAAHVIKDPLPGVPTKEILIWYAMGDCLVTNIATEMIARTMGIDLLAPAVKSVWGMP
                     PKPGPLVNGVNVYDEHRSPLPSDYNIPPSSDNGTHSGVNRNPSALRQVQAFLLQDTIV
                     QTCGGATPAACDCATGACK"
     gene            15702..16337
                     /locus_tag="VFV99_17270"
     CDS             15702..16337
                     /locus_tag="VFV99_17270"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5061122.1"
                     /translation="MTLAEWLDRMLYRQPFEGASARRYAAKERPAFGDLDERLLDGLG
                     PVLAGARRLLDLGAGPATFAARAAARHPQLTVVAVDPSRDFARTHPGVHVVRAVGEML
                     PLGDGAIDVAMCLSSIRHVRDRGATLLELRRVVRGTLVIVELDPAADATRAATHADAI
                     EGALLRRAFSPLVLRTAPPAAAIATLAAQAGWRLRTRRDDPVQPVYLMELQ"
     gene            16425..16643
                     /locus_tag="VFV99_17275"
     CDS             16425..16643
                     /locus_tag="VFV99_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061123.1"
                     /translation="MPDESTVIVRVLEAQVRSATAIADLYGTMREIAELLERTQNAEP
                     SSSVRLELTRADLREQIAELERRIAELS"
     gene            complement(16636..18069)
                     /locus_tag="VFV99_17280"
     CDS             complement(16636..18069)
                     /locus_tag="VFV99_17280"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5061124.1"
                     /translation="MRIVIVGAGPAGLAMGMRLKRAGHTDFTIVERSRGVGGTWHDNR
                     YPGAGCDVPSHLYCFSFAPKPDWQHKFARQPEIEAYFQRCVEREGLASHLQLGTEVHG
                     AEFVDGSWRVRTSSGELTADVLVSGTGQLNRPHIPAFPGLDTFAGTTFHSARWNHDYD
                     LRGKRVVVIGNGASAAQFVPEIAQHVGKLTLLQRTPAYVFPRKDRAYFGVEKWLFKYV
                     PGWRRFYRSWIYWTLESRFTALFQNSVMGQLVKWMMLRHLRKQVADPKLRATLTPNYP
                     VGCKRIVISDDWYPALQRDNVALVTSLLERITADAVITADGATHPADAIIFATGFEST
                     SFLAPMQIVGKTGTTLAAAWQGGAEAHRGVLVSGFPNLFLLYGPNTNLGHSSILFMIE
                     CQVRYVLGCLDQLARRGARWVDVKREAMAHYNDELQQSLTRMTWAADCHNWYKTDDGK
                     ITNNWSGHTTQYWWRTRKPNLDELEIS"
     gene            complement(18087..18728)
                     /locus_tag="VFV99_17285"
     CDS             complement(18087..18728)
                     /locus_tag="VFV99_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061125.1"
                     /translation="MKATVVGATVPAKTLRAALLKADVPTKDVRMIGPRAWVELPHDG
                     RDASLARLAEALATGLMVIDVDLPAPSGEGLQASRATYRGVEADISEEARKLLDERRA
                     DPAAKPLDDAVAASHLAWALVGDADDTASPAEPNVEEQWATSLIGKLVADGLIELRGT
                     HTPIAQLAQVLQTPGRDLGERLLASLIDSTAIDEVYADADQLATLARATRPKR"
     gene            complement(18725..20101)
                     /locus_tag="VFV99_17290"
     CDS             complement(18725..20101)
                     /locus_tag="VFV99_17290"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX5061126.1"
                     /translation="MTARSQILDSILEATGSTPMVRLSRIGRDLPCELLGKCEFMNPG
                     GSVKDRIGVRMLLDAEKSGRIKPGDTLIEPTSGNTGIGLAMAAAVRGYRVIITMPEKM
                     SQEKQVVLEALGAEIIRTPTEAAWDAPESHIGVARRLKEIIPNSHILDQYSNPSNPAA
                     HEDGTGREIIDQCGGKLDAVVMTAGTGGTISGVAKVIKREVPSCKIVGVDPEGSILAG
                     PGEIRTYKVEGIGYDFIPDVLDRRLVDHWIKSNDRDSFLVARQLIRQEGLLIGGSSGA
                     AVWAAMQVCRDLGPGKRVVVILPDSIRNYLTKFVDDRWMRQQGFFKADWEVGTVADIM
                     RSAGRRTVISLDLNDKVSRATDMFKEHGISQMPVLDNGKLAGILTESDLLHQLMTGRV
                     DRETVVAEVMERKVSVVSLHASSGELPRIFERGEVAIVVDDQRAVLGILTKMDLIEML
                     AARKNKMP"
     gene            complement(20157..>21253)
                     /gene="phnD"
                     /locus_tag="VFV99_17295"
     CDS             complement(20157..>21253)
                     /gene="phnD"
                     /locus_tag="VFV99_17295"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5061127.1"
                     /translation="PDCPPGLVGLVEHATARNAADRVPDAATFRRMLDQVVPRQADDA
                     EHWRSFLQPLYRRETFVTRYGVLPYVEDLVPDLSEKEAQPPSVAYVETVVVDTQKPLK
                     FGFSPALGVEAARVQGEKVANWLKKRFDRDVRAVVVSDYPALVDAMVDGELDFAWMPP
                     VSFVRAAEQGVGVVALVQRYGRPTYESAIIVRADSPLVSIEDLRDKSIAYVDRESASG
                     YLFAADLIREGLGKPSEVLKEEHFQGSHKAVSDAVRRKWVDAGVTYVVRDATGALVYS
                     GWLDYGAPHEIALRVLATTAPIPCDAIAHRPGLASGLVDRLGRTLVEADDDPEGHIVM
                     KEVFHTTGMMRADLRIYDAVRAAMQRVYTR"
BASE COUNT         3546 a         7074 c         6845 g         3788 t
ORIGIN      
        1 gactgcgcgg cgaccagatc cgcatccaca tcttgatccg cggccgcatc ggtgaaggct
       61 ggaaagacgt cgacgagtat ctgcgcgtgc ccactggcac aacgctcggc aagctcgtcg
      121 aggtcgcgga cgctgcgggc gtgccgctgt cggaggcact cgctaactcg ccgcatctca
      181 ccgatacgtt gatgctcaac ggcgaacgct gcccgctcga ggcaaatcgc gaacgcgagc
      241 tcgccgaggg agacgagatc tatctgctcg caccactcgc cggcggttag agtagaccga
      301 cgagcagcgc accgtagacg cgcaacagca tcgacagatc ggcgtcgtcg aagtgcagct
      361 cggcgccgag gaagaagtcg cggccgaagc gaacctgatc gaactggatc ggcacgtcgg
      421 agccgggcgg attgcccttg cgaatctgca gatagcccgg tttgttcaac gcgtcgtcga
      481 cgcccgcgat cagatagatc gagcggaaca ccgcgagcgc gcccgcgagc ttgacccgcg
      541 gcaccttctg atacgagccg aacatgtcgg cggagaactt gagccggcca ttctgcatca
      601 gcacgtcggc gccgatgccg aacgtcgatt ccttgatgcc agcgcgcacg tcgaacgagt
      661 tgccgaactg cttgccgaac tgcaccgtgt agcgcagccg atcgtggatc tcttggtagc
      721 ggtagtagtc gggcacgccg accatgtcgc tgatctgatc gtcgggcagg ccgccgagcg
      781 gaccgcgctc gagctcgacg agatagaact tgtcggtgcg cgcgcgcacc tcggcagtga
      841 cgtagaagcg cggctgctgc gcgaagacgt tgaactcggc gcgcagaccg agccacgact
      901 tgaatcgcga gaaactcgac gtgccctcgc gcagcgtgtc ggttccctcc tcgatcgaat
      961 cggcgagatg tggatcgttg atcagccggc cgagcgtgcc ctgcgcgccg ctgccgcgac
     1021 cttgatcgat ccgcgacatc agctcgccgt agtcagcagc tggcttgtcg aggccgtcca
     1081 tgctgctgcg cacgctcgcc atgccgtcgt ggaggctgat ggtgagctcg gccatctgct
     1141 tgcgcgccga gtcgatgccg ccctggatgc gatcgagcgt cttgtcgacc tgcgggcgtg
     1201 agcctgccat cgtgttcgcg gcgctcgtgg ctccggcttc gaggcgagcg agcgcttgct
     1261 cggcgcgctc gatcggccgc tcgacattgc cctcgtcgag ccagtggtcg gcgtcgagca
     1321 ttttgtcctc gagcgtgccg agcgcccact tgcggccgta gagcgcgacc tcgtggagac
     1381 gatcgagacc gcgcgtcacg cgtggcatca cgtcatcgat cgcgcgcagc atgcgatcgg
     1441 tggaggtgcc ttcgagcacg cgcgtcaggc attgccccga ctgcaatcgc tgtgcggtcg
     1501 gtgcgccttc atcaccaccg gtcggaatga tctcgacgta gctgtcgccg aacggcgagt
     1561 acgctcgctt cgtcacccac gaatcgacgg gcacgttgat gtcgttctgg agcgtcatgt
     1621 cgatgcgcgc gagtgggcct tcgagcgtca ggcccgtgac ctcgccaact cgcacgcccg
     1681 cgatcagcac gtacgagccg accgcgagcc ggctaccgtc gcggaaccgc gcacacgtct
     1741 tgaacgtgtc accgaccgag gtcgccggca tcttcgaacg gatccacatc gcgacgagga
     1801 ggacgacccc cacgaacacg acgacggtga tcacgcgcga cagccagcgc acgcctcatc
     1861 gtagccgacg gatcagctca gcgcatacaa tcgagcgtga gcgtgatcat ggaaccgtgg
     1921 tcgatcacgt cgaaatcctc gactttgagg tcggcgaggt cctgcggatc gagcaggttt
     1981 gtccactttg ccgtcgtgtg gcggtcgtct tgcgcggtca gcgcgatgtt gccgccgtcc
     2041 gcatggtaca tgccgtactt ttgcagcgcg cgagcaacga ccttcgcgcc ctcggacggc
     2101 agcgaatcaa ccgggtaatc ggcgcgcaac cggaagtgga cgccgtatgc gggcgcgttc
     2161 gtcccgccgg tcgtattcgt accgtgggtc gcgggccgca cgaagccgcg cttgacgcgg
     2221 ttgttcggca ggatgaaccg gatcgcatgc ttgatctcgc ccgcagcaac ctcgtcggcc
     2281 gtgaacaaca gcggcgagat cgggaagccg gccgcatccg cgctcgtgca ctgatcgccg
     2341 cgcagcgtgt cggtgtacgc catgcgggtg ttccacaccg cgaggcaacc accgtagaac
     2401 gtgctgccca cgatgttggc gcgccacatc tcgtagagct tgccgccgtc gccatcgttg
     2461 acgagcaagt ggcaatcgcc gtcgccggtg cactcgtagc cggtctcgcc ttcgacatta
     2521 ccgccgacgg gaactggcat cgcgacgtca tcgcaatcgg gcgaataaaa gtcgcccgtc
     2581 ttcgtgaacg tgcgcatcgg tgttccgctc ggcgcgttca acacgtcgat cgagaaatcg
     2641 atctggaacg tgtcgccgtt gccccagccg ccggccgcgc gcaacgccgc gatggtcgcg
     2701 tcgctgtagc tcgccttctg cacgcccgag acgtcctggt tgaagaacat cggcacgctg
     2761 aagtacgggc cggtgcccga cgcaaatggg ggcgcatcgt gctggccgcc gtcgggatcg
     2821 ttgacgtcgt catcaccacc accgccgcct ccactaccac caccacagcc aaacagcagt
     2881 gtcgcaagcg cgacgcttgt tgccacccgc atgcactaag gctctcacaa agcctccagc
     2941 gcataaatgg gaacgtttcg gacgacgttt gcagtcgctg tcagcgttgg cagtcgttgg
     3001 tgatgggaat tccctcgccg tgatcgacga cttcgaaatc gctgacctcg agaggataga
     3061 gatcctgcgg gcctagcagt ccatcccact tcgcggtcgt gtacttgtcg ctctgcgctg
     3121 tgagcgtgtc ctcgcctccg tccgcgtgat acatgccgta cttctgcagc gcacgcgcga
     3181 ccacctgcgc gcccgtcgtc gtcagccgct cgacggggta atcagcacgc agtcgcagat
     3241 gaacgccgta gaacggcagc tcgaggccac cgctcgtgta cgcagcgtgc gtggcggggc
     3301 gtacgaagcc ctgcgtcacg cgatcgtttg gcagcacgaa ccgaatcgca tgttcgatcg
     3361 agcccgaagc gacttcgtcg gcgttgaaca agagcggcgc gatcggtaag cctgcaccat
     3421 cggcgctcgt gcactgatcg ccgcgcagcg tctcgtcgta cttgcgcgtc agatcccaca
     3481 cggcgaggca accaccagag aacatcgtcg agatgctcgc cttccacatc tcgtagagct
     3541 tccggttctt gtcgtcgagc acgatcagat ggcagtcgcc gcccgccgag catttgtagc
     3601 tcgtctcgcc ttcgacgttg ccgatcgccg gcaccggtac cggtatgtga tcgcaatccg
     3661 gtacctgaaa ctgatccgtc ggcgtgaatg tctgcggcgc cgacgcatca gcgtgcagca
     3721 cgtcgaacga gaaatcgacg cgcaagtgat cttgattgcc ccaacctccg gccgcgtgaa
     3781 tcgccgcgat ccattcatcg ctgttcgccg ctttcgggac gttcgataca tcctggttcc
     3841 agaacatcga tcgttcgaag aacggccccg agcctgtgtc gtcgccgccc ggtgcgtcgg
     3901 tcgcgcacgc cgtaacgacg cacaagggag cgatcatccg tgccaatgcc atgcgcgggg
     3961 ataggagcaa tccgcgcgcc gtttgctatc gcagccgggc cgtcgctact tgccgcgtcg
     4021 cagatagaaa tgcacgccgc gctgcgggtg cgaaagccgg atcgcgagtg cctgcggacc
     4081 tgtggtcggc gtcaccttcg cggtgaaccg ccgcgtgttc ttgtcgatcg gaatctcgac
     4141 ggcgtcgacc ttcgcgctcc agcccggcag cacggcacct ttgacgtcga cctcggcgcc
     4201 gaacggctta ccgttctgcg gctgctcgat gtagacctgc ggcgccgtct ggtcgaagtt
     4261 gatgatcagc gtcgacgcct tgtcctgttt gacgccgtcg cgctcgaccc agtacgtgta
     4321 cgtcgcttcc ttgagcttct tgccgtcgag cgtgaacgtg ggcttctcgc tgtcgaacgt
     4381 ctcctcggtg ccgctcgtcg cgaggtgcag cttgagcttg cccggaccgc ccggagcttt
     4441 gaccttcacg ttcggaatca gcgactgata cgagatgcga tacgtgcggc catccgcgtc
     4501 gatcggattg accggcggat ccttcggcag cgggcgcgtg cccgagtcct tgcgcacgat
     4561 gatacgcccc gagccgccgg caccgccgcc cgagcacacg agccgatacg accacgcgcc
     4621 ggcttcggcg atgatgtttg ccgattccct gccaccgctg atgcgcgcgg tgcggaagcg
     4681 cggatcgttg tccatctcga tcgttccgcc gccgttgcac ttaccgttga atgcgaactg
     4741 cagtgcggtc gcgcccttcg gatcgtgaat cgtgaacgtc ggcgtctcgc cgatcatcac
     4801 gcgcatgtcg tagtagtcgg gaatcttcgc ggtctggtgg atcgcgccgg ccttttgcat
     4861 cgacgcgctc tcgccggtct tcatctcgag gtcggcgccc gagctgccgg tgagcttcgc
     4921 cgtgccgtcg agcaccgcga tcttcgcctc gcccttggca ttgacgtcga ggcgtgcttt
     4981 gcccgggccc ttctcggtgc ccttgatgcc gacggtaccg cccggcacgc caaccttgcc
     5041 ttcggtctgc gcttcgaccg aagcagttgc gacgccaagc tcgagaccaa aatgcagatc
     5101 gtcggcaatc gcggtgcgcg agccaccagc catcgacagc gtgacgccgt tcgcgatcag
     5161 cttcgcggtg gtgccgttgc cgagccggac cttcgcgccc ttcacgagct gccccgcacc
     5221 cgcaggcagt ggggcccact tcgtttgacc gggctgcaga atctcggcct tcttgccggt
     5281 gacctcgatc gttccgtcct cgctgacgat gacacccgca tcggtgccgc cgtcgcccgc
     5341 tgcgtcgacg ccagcatcga tcggcgtctc gagcttgatc ggaccgatcg tcaggtcgag
     5401 atcgaggacc ttgccgatct cgagatcgag tgtttggccg cccttgtcga tctgcgcgtt
     5461 accgacgagc agctcgatcg tgctcttgcc ctgccccttc gcggcgatgc gaacctcgcc
     5521 atcgttgccg agcttgacgt cgccgacatc gaggccgtag ttgccgccgc ctttgagcac
     5581 gatcgcgccg agctcgaccg tgatcttcgc ggagccgtcc ttgccgccgc caaagcgcag
     5641 cacggtgtac ttctccatct tgatgagctg aacgccgaca agcttcagct cggcagcgcc
     5701 atccgccgtg cgcgcggcat cgccaatatg aaacttctgg ccgatattcg cgccgcccca
     5761 cgggccctga ttctcttgcc gctcgaccgg gccgtcggcc ttggtcagct ccgcgatcgt
     5821 cggcttcttc ttgcagccaa acgacagcgc gagcacgagc aggcagacca cacccgtcgc
     5881 acccctccgg cgaagcagtt gccagctact tctgcagatc gatgttcttg atcgcgacac
     5941 cgttaggatc gatcgtgacg tcgagctctt ggtctttgag acctgccttg ctcaccttga
     6001 tcttgtactg acccggcgcg aggtcgatcg tgaacgtgcc gtcgtcgccc gtcgtcgcct
     6061 tcgcttcgct gcccgcgacg gtcaccgttg caccacccac tgccttacca cccggaagcg
     6121 agcgcacgac accgcgcagc tgacccggcg gcaacaccgg ctcgagcgcg atcggcggca
     6181 ccgtgttcga gccctcggcg atctgcgtga tcgtcgattt gccgggcttc atgttgctga
     6241 cttcgacgcc gacctcggcc gcggtctctt cgatcgtcgg cttgccgccc atcgagtgac
     6301 cgatgagcac gcccttgaac acgtacgttc ccttctcgtc ggtgacgacc gggttgacct
     6361 gcgcgttctt gagcgtcagc gtcaccttcg cgccaaccac cggcttgccg ccgttgtcga
     6421 cgaccgtacc ggtgacctcg gtgaggatgt ccgccttgac gtccgggcaa tccggcggcg
     6481 tgtgacgcgc gcactcgcgc tcggtgaagc tcggcggcac ggccttcgga ccgccgaagc
     6541 gcgcctcgaa accgatgcct gccgtgatga tcggctcgta gggcacgagc ggaatgttcg
     6601 cgttgtccac ctgcgagtcg ttgatgccgg gcaccttcgc cgcctcgagg aacgcgaccg
     6661 cggagtactg atcggtgatg tggaagccgc cggtgatgcc accgcgcatg atcaacttcc
     6721 cgcgctcgag cgatgcacgt ttgacctcgc ccgcggcggg atcaggcgga ccgccgacga
     6781 atgcgtcgat cgagccttcg acagagaccc acgccttgcc ggcggggatg cgcagctgcg
     6841 cgccgccaaa cagcgcgtga taatcggaga cgccgagcga cacgcgatcc ggaagcgaga
     6901 gcttcatcgg atcgtcgacg ctattcgcgc tgttatcgag acggaaaccg ccgttgaacg
     6961 agagcagacc cgggcccgcg ggcagcgaca cgagagcgcg aacgtcgatc gagatcgccg
     7021 agccggcgac cgacggcgca tccttgcccg gcacccagat gccgagctga ccacctgcga
     7081 tcaggctacc ggtgttcttg ctggcgcgca cgatcaggtg cggatcgccg acgtagccgt
     7141 cttcgttctt gccgccaggt gccgccggaa ccgtggtcgg atctccgagc atcggatcgc
     7201 cgagcgttcc ccagtggcgg tcgtagcgac cgtcgagcga gatgccgatc gacaggtacg
     7261 gggtcgcgcc aaacgcgatc gctagatcac caactccacg attgaatttg tgatccggcc
     7321 cgagcagtcc agtgcggcga ccaaggccca tgagcgtcga aacttccacc gcacccttgg
     7381 gcaacgtctc cgctgttggc gcgtgcaagc cgcccggcat ggcgccgtag acgatgtctt
     7441 gcgcgggcgc cacggattga tccgtgctgg tgccagccga tatctctgcc tcttgcgcgg
     7501 agacggtcag cggaaccaga agcacggaga gaaacgatgc gaatccccac cggcaacgca
     7561 ttaccgaaag tttaagcgct ggactcgggt aattaccagt gcgccgccag gttttggggc
     7621 acttcgataa cctggcgcta gtagcgcagc caacgaacta gctcacgcat gctcggccgc
     7681 tttccgtaca tcaggatgcc aacccggtag atctttgcag cagcacgcgc aacgaggact
     7741 gtcgcagccg ccagaatcgc gagcgacagc ccgacctgcg caagactcgc gcctccgagg
     7801 aagtagcgca gcggcatgag catcggtgac cagaacggca ccatcgtcat gatctgagcg
     7861 gcttggccgc ggggatcgtt tgtgacggcg gtgatcgcga tgatgccgat gacaagcagg
     7921 aacgtcaccg gcatctgggc ctgctgggag tcctgctccg aggagaccat tgcacctact
     7981 gcggcataca gcgcggaata gaacaggtag ccaagtacaa agcacgcgag cgtgatcgcg
     8041 agctggatcg gggtgagcga cggcatcatc gacgtcgcat cgggcttggc cccgaacagg
     8101 ccgagcaggg tctcgcgctg cgatatcacc acgtaaccga tcacgaacca gatcgcgatc
     8161 tggagcaggc cggcgaagcc gacgccgagg atcttgcccg acatcatcgc gcgtggcttc
     8221 gtcgcggcaa cgagcaactc gaccacgcgc gacgtcttct cggtgacgac gctgcgcatc
     8281 acgttgatgc cgtacagcgt gatcaggatg tagatgaaga acgcgatcat gtagccgacg
     8341 aggaacgcga agatgccagc ggtgccctgt tcttcaccgg tcgtgtggcg cacgctgacg
     8401 ttgaccggta gtttcaacag cgcgatgtcg acttcgctga ggcccgcgcg cttcgcccgt
     8461 tccgtgatca ccattcgctg cgcgagctga ccgaagccca tgctcacggt ctgattgctc
     8521 gcattgtcgc cgttgtaaac gatctcgccg ccgtcgagcg cgtccttcgg gatcacgacg
     8581 tagccgttga tctcgtcgga cttgatcttc gcgagcagag tcttttcatc agtgtcaggt
     8641 ggcgcgacgg tcgtattcca tttgaggaca acgccgagct ggtattgcat ttgctctgcg
     8701 agcctgcccg acttgtcgat gatcgtgacc ttcgcaccct cggtgccttg gttgccgatc
     8761 agcgcgggca cgaccatcat gccgaccatc aagatcggcc acaggatcgt catcacgacg
     8821 aaccacttcg accgcacgcg ctcgtagaac tcgcgcttcg cgatcaccca catctcacgc
     8881 aacggcaccc tcctcgtcgc ggcgcgccgc aaccgccgct tctgcgccga ccttctgcac
     8941 gaagatctgg tgcagcgtcg gcacgacgag ctcgaacaag cgcagcccga tgtcgtgatt
     9001 gagtagcgtc ttgaggaaat ggtccggctt gatgccgtcg gcgagcacga cctcgcaatc
     9061 cgcgtgctcg cctggacgcg gcggcttgcg ctcgatgacg agcttctcgt cggcgagcag
     9121 cggctcggcg cgctccttcg ctgcgtcgtc ggtaaacgcg agcgcgatca cgccctccgc
     9181 gaccgacgcg cgctggatgt ccttgagccg accatcgagc acttttctgc cgcgcgcgat
     9241 gatgcagacc tgatcgcaga ccttctccgc cgattccatc tggtgcgtcg agaacatcac
     9301 ggtgcggccg gagctcttga tctcctcgac gacgtcgacc agcacctcgg cattgatcgg
     9361 atcgagaccg ctccatggct cgtcgaggat cacgagcgcg ggctcgtgaa tgagcgcggt
     9421 cgcgaactgg accttctgcg ccatgccctt cgatagatcc tggaccttgt tcttgcccca
     9481 gtcggcgagc ccgagccgat cgagccactt gtgcgcgcgc accttcgcct cgcgatgccc
     9541 gagcccgcgc agctcgccca tgaacacgat catgtcgatc acgttcatct tcggatacag
     9601 gccgcgctcc tcgggcaggt agccgatctg ctttgccgcg gcatggccgg gcgcgaggcc
     9661 gtcgaggatc gtgatcgtgc cggcgtcggg cgcgatgatg tcgttgatca tccgcagcgt
     9721 cgtcgacttg ccggcgccat tcggaccgag gatgccgaac acgctgcctc gcggcacttc
     9781 gaacgaaatg ccatcgaccg caacgtggtt gccgtacttc ttgaccagcc cggtgaccgt
     9841 cagcgcgggc tttgtcatgc agctccggca gccccggcag ctccggccgt tgcgccggct
     9901 gcatcgccga ggatcgcgcg ctcgagcacc gccatgcgga tcgcgacgcc ccagctcact
     9961 tgctggagga tcacggcacg cggtccgtcc gcgacctgag gcgacacttc gacgccgcgg
    10021 ttgatcggcc cggggtgcat gacgatcgcg tcgggcttcg cgtaggcgag cgtgcgctcc
    10081 gacagaccga acgtgcgcga caactcgcgc gtattcggga agcgcggcgc ctcgccctcg
    10141 aagcgctcgt gctggacgcg caacatcatc accacgtcgg caccgtcgag gccatcctcg
    10201 agccgcgcgc agatccgcgt ccgctcgcgc gtcagctcgc cgccgatcag ctcgatgccg
    10261 cgcgggatca gcgtccacgg tgcgacgagg cgcacgcgag cgccgagctt cgacaagcag
    10321 agaatgttcg agcgcgcgac gcgcgagtgc tggatgtcgc cgacgatcgc gacctcgagg
    10381 ccggcgagct cacctttgtg ccggcgaatc gtcgcggcgt cgagcagtgc ttgcgtcgga
    10441 tgctcgtgct ggccgtcacc cgcgttgacg accgcacact tcgcggtctt cgcgagcagg
    10501 tacgcggcgc cgccgaacga gtggcgcacg acgatcgcgt cgggatgcat cgcctgcagg
    10561 ttgcggccgg tatcgaggag cgtctcgccc ttcgtcgtcg acgacgagct ggccgcgatg
    10621 ttgatcgcct cggcagagag ccgcttcgcc gcgagctcga acgaggtacg cgtgcgagtg
    10681 ctgctctcgt agaagaggtt gatgaccgtg cgaccgcgca ggctcgtcag cttcttgatc
    10741 ggctcgtcgg ccagcgacca gtagcggtcg ccgtgatcga ggatgcgaac gatttctgcg
    10801 gcgtcgaggt cagcgatgga gaccaggtgc cgggggccgc tcgtcacgcc cttcgtatag
    10861 cagatcaagg gtgttatggt ggggcgtgtc cggagcgaag gtgagccgga ttggctcgat
    10921 catcggggat cgcttccgac tcgacgaatt gatcggccgc ggtgcgatgg ccgaggtcta
    10981 ccgcgcgctc gacctcgaga cccaggcgtt cgtggcggtc aagatcctgc gccatcagca
    11041 cgttacggac acggtctcga tcgcccgctt cgcgcgcgaa gccgacgtcc aggcacgcct
    11101 tcgccatcgc aacgtcgccg cgctgctcgc gagcggtgtc acgaaggaca agcacgagcc
    11161 gtatctcgcc gtcgagctgt tgcgcggccg ctcgctgcgc agcgtgatca agtccgaagg
    11221 ccacgtcgtc ccgcgccggg ccgcgagcta cacgtggcaa gcgttgcagg gcctgcatgc
    11281 ggtgcatcag gcgggcgtgc tgcaccgcga tctcaagccg gcgaacatca tgctcgagcc
    11341 gtcgcccggt ccgatcgagc gcgtcgtgct gatcgatttc gggttcgcga ccttcgaggg
    11401 cagcgcgcga ctcacgttgc aaggcacggt cgtcgggagc ctcacgtaca tcgcgcccga
    11461 gcgcttgcgc gccgaagagc ccgaccagcg cgccgacatc tacgcggtcg gcgtcgtgct
    11521 gttcgagctg ctcaccggcc agccgccatt tgccgccgag gaagatttcg atctgatcag
    11581 catgcacctg cacgatccgc cgccgagcct ggcccacgtg gtgccgagtg cacgcgcgct
    11641 cgatcccgtg ctcgcacgtg cactcgcgaa acacccggcc gaccgctacg ccgatgcgca
    11701 gcagatggcc gacgatatcg agcacgccgc gaagcagctc acagaatgac gatcggcttc
    11761 aggtcgaggc ccccagcgaa catgtacgaa acgccgtcgg agtagccgta cacggtgatg
    11821 ccgcagccat cggcgcacgt gatcttgtgc tggccggccg gtaccgtcac gcgcgcggcg
    11881 cgatgcgtgc cgccgcctgg aaacgccgtc atcgtgaccg gcgcgccgtc gagctgcaca
    11941 ccgcccgtcg ccgccgctgc gattgaaatg tagttcgcgg tgtactgcgc gggcacgagc
    12001 accgtgtact ccttgcggaa ctgctcggat ggaaccgcga tcgccatcga cggatcgccg
    12061 gtaccgacga agctgttctg cccgttgttc caccacgtcg ccgactcgag gaagtgaccg
    12121 acgagaatcg gctgcgtcgc gctgatctcg gtgtcgccct ggatcttcac gtcgcagaac
    12181 tcgccagccg cgagcgcgct gcagttgccg tagaccgacg cgctcggcgc cggcgtgaac
    12241 gtcacggcag tgcccggctt ctgcgcgagg atgcgcaggt aatcgggctc gttcgagcgc
    12301 tgcttgctgc gcgcgatcgc gaacgtcatg ccccacgtcg tactcgggaa cagcatctcc
    12361 tcgaggtgat cggcgaagcc ggggccgttc gggtgcatgc tgtcgggcgc cgtcgtctcg
    12421 ccgaatgctg tcgcttcgtg gccgccgtaa acgccgaacg gcatcatgtt cggtgacgtg
    12481 atgtgcgtgc cggtcagatc gccactcggc gccgtaccgg tgccaaccgc ggcttcgagc
    12541 tgcagcactt caaatgcgtc gagcgtgaac aacacggtcg cgccggggac gagcgtcgcc
    12601 tgcgtctgac tcgcaacgac agccgtcttc ggcgtcaccg cgatctgcgt gccgtcctgc
    12661 ggcgcgacga tcgtgagata gccgtagtac ggatcgcgat tcggccggcg attcagcgtg
    12721 acccagctca tcccgtagta gtcggtgtcc atcgtcgcgc gcgggatcaa cagcgacgcg
    12781 tcgttggaga acacgttcga gttgtcgagc gggttgaact gataggcgac gaccggcaga
    12841 tccgatgtga tgcggtacgc cttcttgaac ttgccggtgc catcgacgga ctgatctgga
    12901 atcgcgttgt tcgcctgcat caagatcggc gtgacctgac cagcggtgac gaccttcgtg
    12961 atcgtctgcc cgcccgggcc ggtcaccgtc accgtggcgt cctttgcctg cggattcgac
    13021 accacgaccg cgaacggacc gtgctgcgcg tcgctcgcgc agacgagctt cggcagacac
    13081 gtcgtccctg ccgggcacgt gtgtggcgcc atgctgtcgt tctgcggatc gcactggccg
    13141 aggtacaggc caccgcccat catcgtgtag caaacgttga gcgtcatcgg cgagccctgc
    13201 gcgcacgtgc ccgacgagcc gccccagacc tcgaccgcat tatcgagatc gaccgcccag
    13261 tactcgcaac cgatgtagct cttgctcgcg gcagcatccg cgcacgcatc gacgcacgcg
    13321 ccgccgacgc acgtgttggc gccgccgcac acggaaacct cacagcccgg cttgccggcg
    13381 tcgtcgcaga cccagacgtt gccgtccttg cagaagttct gtccgggcgt gcactcgacg
    13441 caaccgagcg agtcactgca caacgcagga caatcgatcg cggtctgcca ctgaccgccg
    13501 tagcacgtct ggtacgacgc accttcgcaa cggtgcgcgc ccgacggcgc acagttgttc
    13561 agcgcatcgg gactggtgtc atcgccgctg ttgcgtggcg cgttaccaca gccagccaac
    13621 gcgacgatgc acgtgaacag cgcgacccgc ggggatccct ccacgataca aacgtatcac
    13681 gcgggttcgc gtcagttccg tgctcacttg cacgcaccgg ttgcgcagtc gcatgcggcc
    13741 ggagtcgcgc cgccacaggt ctggacgatc gtgtcctgga gcaagaatgc ctggacttgt
    13801 cgcagtgccg acgggttgcg gttcacgccg gagtgcgtgc cgttgtcgct cgacggtggg
    13861 atgttgtagt cggacggcag cgggctgcgg tgctcgtcgt aaacgttcac gccgttgacg
    13921 agtgggcctg gcttcggcgg catgccccac accgacttca cggcaggcgc gagcaagtcg
    13981 atgcccatcg tgcgcgcgat catctcggtc gcgatgttcg tgacgaggca gtctcccatc
    14041 gcgtaccaga tcaggatctc cttcgtcggc acgccgggca gcggatcctt gatcacgtgc
    14101 gcagcggtcg tgatcggatc gtagggctcc atgcccatac cgaggaacgc gaggttcagc
    14161 tggtagacct cgggatcctc gtacgcaccc tgtgcggcac ccatgagcag gtgccacgcg
    14221 ttcgagcgct cgaacatcag cgaccacacg ccgcctggca ccgcgagcac gcccttcgtg
    14281 atgttcggat cgtaggccat gaacgtgttg cccatgattc cgccgagcga accaccgacg
    14341 tagaacgtct tgttcggatc gatcacggac gcgccgttgt actggaattc gggatgctga
    14401 ccgagctggc cgcgcgtgat cgtctcgagt gagatgaagt cgatcaccga ttgcgcgagc
    14461 ttctcggtga tcgccttgcc cttgttcatg tcgttgacgg cgagcggcgc gagcgagagc
    14521 tggcgatcgg tgaggccgat gaagtcgccg gcgaggatca cgaggcagta gtcctgcgcg
    14581 aggttgacga cgaagttgtc gtcgagatag ccctcggcag agccgaacaa gccgtgaccg
    14641 aagatgatcg tcggcctcgg cagcggctgg gtcgtcacgc actcgggaac gatcgcggca
    14701 aaccgcgcgt cgcgcaggcc catcatctgc ggcagattgt tcgcgtcgcg gcgcatcacc
    14761 gagctgtctt cttcgccgtt ggtgaggaag tcgggcgact tgaacgtacc ggtgaagctc
    14821 ttcagcacgc cggttcgtgg cgcttgctgc gtcgcagtga acgtgaggtt cgcgccgttt
    14881 tggcccattg cgggaatcgc ggcgtcgcgc atcgtcgtca gatcgcgctg caggaactcg
    14941 tccgacgcgg tgtagtagtc ccacgcgagc acgagctcgc tcttgtcgac gcccgccgtc
    15001 gcgagcgccg cgaagatctt ctcgtaacgc tccttcgagt agcgcgagtg accgtagccg
    15061 ctgccggaga gcgccttcgc gaatgcttcc ggaatcggca agtcgctgcc gtcggcggcc
    15121 ttcacgctgt tgcggatcgc gaccgcgtag tgcgcgccgg gcgcaaggcg cgcgagcgga
    15181 cggatgatga ggttgcgctt ctcgatgtcc gcggtgttct ggtcgacctc ggcgaagaac
    15241 ggcgcacgct cgccggtgtc catgttcagg agcacgatcg gcgagtcagc ggcgagcgat
    15301 tcatcggggt tcttccacga cggcaagttc gtgttcgaca cgccggtcgg aaacgcggcc
    15361 agcatcggtc cggtcggcga gaagccgtcc cagcgatcga gccacgccgg atcgatcgag
    15421 acgtcgtcga tgttcttcgg catcgcttcg atcggaacct gcacgcggcg cccggtcgcc
    15481 gtcgttgcat cctcgacctc gtacgcggcc gacggccacg gcaacaggca gccttggccg
    15541 cccatcgggt tgcattgatc cggaatggag ctgccgtcgc taccgcacgc ggcaagcgaa
    15601 agcgccacca aaaccacatg gcgtgggtcc ctcatcacgg cggaacctat caccggcgcc
    15661 ctgcggcgcg tgtgaggaaa ttcgcaggct atgctcgtcg cgtgacactc gcggaatggc
    15721 tggaccggat gctctaccgg caaccgttcg agggcgccag cgccaggcgg tacgcggcga
    15781 aagaacgtcc ggctttcggg gacctcgacg agcggctgct cgacgggctg ggcccggtgc
    15841 tcgccggtgc gcgccggctc ctcgacctcg gcgccggccc ggcgacgttc gcggcgcgag
    15901 cggcggctcg tcatccgcag ctgaccgtcg tcgcggtcga tccgagccgg gatttcgccc
    15961 ggacgcatcc cggtgtccat gtggtgcgag ccgtcggcga gatgctgccg ctcggagacg
    16021 gcgcgatcga tgtcgcgatg tgcctctcgt cgatccgcca cgtgcgcgat cggggcgcga
    16081 cgctgctcga gctccggcgc gtcgtgcgcg gcacgctcgt gatcgtcgag ctcgatccgg
    16141 cagccgatgc cacacgcgcc gccacccacg ccgatgcgat cgaaggcgcg ctgttacgcc
    16201 gcgcgttcag tccgctcgtg ctgcgcacgg cgcctccggc cgccgcgatc gccacgctcg
    16261 cagcgcaagc cggttggcga ttacgcaccc gccgcgacga tccggtgcag cccgtctacc
    16321 tcatggagct gcagtaaccg gctcgaggag cggttggaaa cttctcgttc tgaaccgaaa
    16381 aactcgcgag tgcgattgcg accgttgccg cgtacgatca acgcatgccg gacgagtcaa
    16441 ccgtcatcgt tcgcgtactc gaggcccagg tgcgcagcgc gaccgcgatc gcggacctct
    16501 atggcacgat gcgcgagatt gctgagctgc tcgagcgcac tcagaacgcg gaaccgtcgt
    16561 cgagcgtgcg gctcgaactg acaagggctg atcttcgcga gcagatcgct gagctcgaac
    16621 gaaggatcgc cgagctcagc tgatctcgag ctcgtcgagg tttggcttgc gggtacgcca
    16681 ccagtactgc gtggtgtgcc ccgaccagtt gttcgtgatc ttgccgtcgt cggtcttgta
    16741 ccagttgtga cagtcggcag cccacgtcat ccgcgtgagt gactgctgca gctcgtcgtt
    16801 gtagtgcgcc atcgcctcgc gcttgacgtc gacccaccgc gcgccgcgcc gcgcgagctg
    16861 atcgaggcag ccgagcacgt agcggacctg gcactcgatc atgaacaaga tcgacgagtg
    16921 gccgaggttc gtgttcgggc cgtaaagcag gaacagattc ggaaagccgc tgaccagcac
    16981 gccgcgatgc gcctcggcac cgccctgcca cgccgccgcg agcgtcgtgc cggtcttgcc
    17041 gacgatctgc atcggcgcga ggaagctcgt cgattcgaaa ccggtcgcga agatgatcgc
    17101 gtcggccggg tgcgtggcgc catcggcggt gatgaccgcg tcagccgtga tgcgctcgag
    17161 cagcgacgtc acgagcgcga cgttgtcgcg ttgcagcgcc ggataccaat cgtccgagat
    17221 cacgatgcgc ttgcagccga ccggatagtt cggcgtcagt gttgcgcgca gcttgggatc
    17281 ggcgacctgc ttgcgcaggt ggcgcagcat catccacttc acgagctgac ccatcacgct
    17341 gttctggaac agcgcggtga agcgcgactc gagcgtccag tagatccagc tgcgatagaa
    17401 gcgccgccaa ccgggcacgt acttgaacag ccacttctcg acgccgaagt acgcgcggtc
    17461 cttgcggggg aacacatacg ccggcgtgcg ctgcagcagc gtcagcttgc cgacgtgctg
    17521 cgcgatctcg ggaacgaact gggcggcact cgcgccattg ccgatcacga cgacgcgttt
    17581 gccgcgcaga tcgtagtcgt gattccagcg cgccgagtga aatgtcgtgc ccgcgaacgt
    17641 atcgagtccc gggaacgccg ggatgtgcgg gcgattgagt tggccggttc cgctgacgag
    17701 aacatcggcg gtgagctcgc cgctcgacgt acgaacccgc cagctaccgt cgacgaattc
    17761 ggcgccgtgc acttcggtgc cgagctgcag atgcgacgcg agcccttcgc gctcgacgca
    17821 gcgctggaag tacgcctcga tctccggctg gcgcgcgaac ttgtgctgcc agtcgggctt
    17881 cggcgcgaac gagaaacagt agaggtgcga gggcacatcg cagccggcgc ccggatagcg
    17941 gttgtcgtgc cacgtgccgc cgacaccccg cgaacgctcg acgatcgtga aatcggtgtg
    18001 gcccgcgcgc ttgaggcgca tgcccattgc gaggcccgcc gggcctgcac ccacgatcac
    18061 gatccgcatg cagcgggcat catacgtcag cgcttcggtc gcgtggcgcg cgcgagcgtg
    18121 gcgagctggt cggcgtcggc gtagacctcg tcgatcgccg tcgagtcgat gagcgacgcg
    18181 agcaagcgct cgccgagatc gcggccgggc gtctgcagaa cctgggcgag ctgcgcgatc
    18241 ggcgtgtgcg tgccacgcag ctcgatcagg ccatcggcga cgagcttacc gatcagcgag
    18301 gtcgcccact gctcctcgac attcggttct gccggcgacg ccgtgtcgtc cgcatcgccg
    18361 accagcgccc atgcgaggtg gctcgccgcg acggcatcgt cgagcggctt cgcggccgga
    18421 tccgcgcgtc gttcgtcgag cagcttgcgc gcttcttcgc tgatatcggc ttcgacgccg
    18481 cgataggtcg cgcgcgacgc ctgcaggcct tcgccactcg gcgccggcag gtcgacatcg
    18541 atcaccatca ggccggtcgc gagcgcctcc gcaaggcgcg caagcgatgc atcgcggccg
    18601 tcgtgcggca gctcgaccca tgcgcgcggc ccgatcatgc gaacgtcctt cgtcgggacg
    18661 tccgccttca ggagggcggc gcgtagggtc ttcgcgggga cggtggcccc gacgacagtt
    18721 gccttcatgg catcttgttt ttacgcgccg cgagcatctc gatcaaatcc atcttggtga
    18781 gaattccaag aactgcacgc tggtcgtcga ccacgatcgc cacttcaccg cgctcgaaga
    18841 tgcgcggcag ctcgcccgag ctcgcatgca gcgaaacaac cgagacttta cgctccatga
    18901 cctcggcgac gacggtttcg cggtcgacgc gccctgtcat cagctgatgt aggagatcac
    18961 tttcggtcag gatgccggcg agcttgccgt tgtcgagcac gggcatctgc gagatcccgt
    19021 gttccttgaa catgtcggtc gcgcggctga ccttgtcgtt gagatcgagc gagatcacgg
    19081 tgcggcggcc ggccgaccgc atgatgtcgg cgaccgtgcc gacctcccaa tccgccttga
    19141 agaagccctg ctggcgcatc cagcgatcgt cgacgaactt ggtcaggtaa ttgcggatcg
    19201 agtcgggcag gatcacgacg acgcgcttgc ccggaccgag gtcgcgacag acttgcatcg
    19261 cggcccacac cgccgcgccc gacgagccgc cgatcaagag gccctcctgg cgaatcagct
    19321 ggcgcgcgac taggaatgaa tcgcgatcgt tcgacttgat ccagtgatcg acgagtcggc
    19381 gatcgagcac gtcggggatg aagtcataac caatgccttc gaccttgtag gtcctgatct
    19441 cgccggggcc ggcgaggatc gagccttccg gatcgacgcc gacgatcttg cagctcggca
    19501 cctcgcgctt gatcaccttc gcgacgccac tgatcgtgcc gccggtgccg gcggtcatca
    19561 cgacggcgtc gagcttgccg ccacactgat cgatgatctc gcggccggtg ccgtcctcgt
    19621 gcgccgccgg gttcgacggg ttcgagtact gatcgagaat gtgcgagttc ggaatgatct
    19681 ctttgagccg ccgcgcgacg ccgatgtggc tctcgggcgc gtcccacgca gcctcggtgg
    19741 gcgtgcgaat gatctcggcg ccgagcgcct cgagcacgac ctgtttctcc tgcgacatct
    19801 tctcgggcat cgtgatgatc acgcggtagc cacgcacggc ggctgccatc gcgagaccga
    19861 tgccggtgtt gcccgaggtg ggctcgatga gcgtgtcacc cggcttgatg cggcccgact
    19921 tctccgcatc gagcagcatg cgcacgccga tgcgatcctt gacggagccg ccggggttca
    19981 tgaactcgca cttgccgagc agctcgcaag ggagatcgcg gccgatgcgc gagagccgaa
    20041 ccatcggcgt gctaccggtc gcttcgagaa tcgagtcgag aatttgggac cgcgcggtca
    20101 tggcgcgcgc agtctatgcc gatgcggcgg ggttgtctct accgcgcgta cacgctctat
    20161 cgcgtgtaca cgcgctgcat cgctgcgcgc actgcgtcgt agatgcgcaa gtccgcgcgc
    20221 atcatgccgg tcgtgtgaaa aacctctttc atgacgatgt ggccttcggg atcgtcgtcg
    20281 gcttcgacga gcgtccggcc gagccggtcg accaggccgc tcgcaagccc tggccggtgt
    20341 gcgatcgcgt cacatggaat cggtgcggtg gtcgccagca cgcggagcgc gatctcgtgc
    20401 ggagccccgt aatcgagcca gcccgaatag acgagggccc cggtcgcgtc gcgcacgacg
    20461 taggtgacgc ccgcgtcgac ccacttgcgg cgcaccgcgt cgctgaccgc cttgtgcgag
    20521 ccctggaagt gctcctcttt gagcacctca cttggcttgc cgagcccttc acggatcaaa
    20581 tcggcggcga acagatagcc gcttgccgat tcgcgatcga catacgcgat cgacttgtcg
    20641 cgcagatctt cgatcgacac gagcggtgaa tccgcgcgca cgatgatcgc cgactcgtac
    20701 gtcggccgac cgtagcgctg cacgagcgcg accacgccga cgccctgctc cgccgcgcgc
    20761 acgaatgaca ccggcggcat ccacgcgaag tcgagctcgc catcgaccat cgcatcgacg
    20821 agcgcgggat agtcactgac gacgaccgca cgcacgtcgc gatcgaagcg cttcttgagc
    20881 cagttcgcga ccttctcgcc ctgtacgcgc gcagcctcga caccgagcgc cggcgagaat
    20941 ccgaacttca gcggcttctg tgtgtcgacg acaaccgtct cgacgtaagc gacggatggc
    21001 ggctgcgcct ccttctccga tagatcggga accaggtcct cgacgtaagg aagcacgccg
    21061 tagcgggtga cgaaggtctc gcggcgatac agcggctgga gaaacgaccg ccaatgctcg
    21121 gcgtcatcgg cctggcgcgg cacgacctga tcgagcatgc gccggaacgt cgcggcatcg
    21181 gggacgcgat cggcggcgtt gcgcgcggtc gcgtgctcga cgagcccgac gaggccgggt
    21241 ggacaatcgg gcc
//
LOCUS       DASUFX010000431        12606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_487864,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000431 DASUFX010000000
VERSION     DASUFX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487864"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFV99_17300"
     CDS             <1..989
                     /locus_tag="VFV99_17300"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5061128.1"
                     /translation="VVATRMTEIPGPIRLCYEGAVTRLAGEAGQREILQAGIELVDAP
                     EPDGDAEVLAVAAAALARIALPEIRIDIGHVAPAAFVLGSAPDPHARALITNSLARKD
                     RAGLRAAARVLPEGVAPLAEALATLWGPADATLDRAAALPWPAPVKAALDQLRAVLAA
                     FADLAEAPPPVTIDLGDLRGFDYYTGVRFAAYAGGAPDAVLRGGRYDELLARYGRKAQ
                     ATGFAIDLEAVAQAQRSIGIAPPTAALGVATHGAGAGELARVLRRAGLRAVTQSRAPA
                     SWGAWLRGAGLEAVLIGDQLIGIDDNPRSAKAAIEAARAGDTTALIALIKAT"
     gene            990..2294
                     /locus_tag="VFV99_17305"
     CDS             990..2294
                     /locus_tag="VFV99_17305"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5061129.1"
                     /translation="MSVVIVVGAQWGDEGKGKVVDLFTERADVVVRYGGGANAGHTLV
                     IDGKKLVTHLVPSGVCHPGKSCVLGDGMVIDPHTLLEEISECQARGLLCQQELLIGLG
                     AHVILPYHKLIDGLREDFGAKRGKAIGTTRRGIGPAYEMKAARRGVRMRDLLRPERLR
                     ALVAANLDDLAPVITHLGGAVPSTAEVGTWIDDALAAGEKLRAYTGDAGRHVARAIAE
                     GRHVLFEGAQGCLLDLDHGTYPYVTSSSTIAAGACQSAGIGPTQIDAVVGITKAYATR
                     VGEGPFPTELHGEAGERLRKAGGEFGATTGRPRRCGWLDLPALRMGVRLSGMQSLALT
                     KLDVLAGLPEVSVCVSYSLDGKELDEPPTDPEDIARAQPVYKHFKGWDKLPSEAHEIG
                     DLPAHARAYVQTISEMAGVPFCLVSVGPDRSETIRLTDPFAG"
     gene            2385..4163
                     /locus_tag="VFV99_17310"
     CDS             2385..4163
                     /locus_tag="VFV99_17310"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEX5061130.1"
                     /translation="MKIVCDACQAKYSISDDKVQGKVFKIRCKKCSNIIVVRGGAGAS
                     EPAPAPQEKDTRVYDYGYDGAGGAPAADDAVWHLVINQDQVGPLTVAEVQQRFASGEV
                     DGETFTWREGFADWLPLSQVDTFAALVASGSTTTAGGGGAAAVSAMFGGGDDMGGTAR
                     SDPGDLFAAASSSAKSSRGHEDDDGGDLFGSSKPAAASAQSAAATAKLRGERNENSVL
                     FSLNNLAQLASDSPRPAPSSSGGGGGGSSAGHATGAAGGEGSGLIDIRSMASAYMGGG
                     GAGAAMGAPKSASAIGSIDDLPVFGGGGFSEPAVIVPTARGASNNKMLYIMIAAVGLL
                     AVVAIVMVVLLLKGGKKDGDKVAANTATEKTDKAATDPTEKPTEGSAATGSAEKPAET
                     GSAAVAATEPEKKEPEKKEPEKKEPEHKATKTTSSTSKSSGGSTTPKTTVAKTETKTE
                     TKPAGGGGGDSGGCDEVSCVLNNYEGACCAKFKKGGKPSGGGGGGGGGGGGAKSDLPD
                     SLDRAMISNGIAAVKARVTTCGDKSPAKGKVKVKVQVSGDGRVTGVSVETTPDPALGA
                     CVSAAVNKASFAKTQHGGSFSYPFVF"
     gene            4348..5877
                     /locus_tag="VFV99_17315"
     CDS             4348..5877
                     /locus_tag="VFV99_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HEX5061131.1"
                     /translation="MAWIAQLPAAIMRISLVTVLTSLPLFIAACAGGEDGPTPYDDDY
                     GITSGNINEGAPDNDSLPDDNKADAQYPDQYELPKAEQSVVKSQGSRGVCSIFAATAV
                     VENLYLKAGMPTVDVDFSEQYMQWSVKNQVGAFRNTEGSNVDSNLQAVVRYGGIKEAA
                     WAYESFPWSAANDPACTGGENLPTKCYTNGEPPQSALDAQKFKLPSSRWINTNSIKAH
                     LTTKKTGVGVGMTFFYQSWNHRRSTLPVDADLWRQGVVTYPNADDKTASLMQRAGHAI
                     EIIGWDDTKEVVMRDKDGHPVLDTNGQPKKEKGFWLFKNSWGTASFGVDHPYGAGYGW
                     LSMKYVAEYGSAVVAEVPSLAPAREVCDDAPKADEDGDGKANCDDTDCAMHPSCTSSG
                     GMAHTYTASPNASIPDNSPTGASSAINVSDSGTIVDVKVTTDITHSYRGDLKVTLSHG
                     ATSQVIVDGVGGSADDLKQTFTVTGFSGELSGAWTLKVEDTASQDVGTLNGWTLEVTS
                     R"
     gene            5896..7437
                     /locus_tag="VFV99_17320"
     CDS             5896..7437
                     /locus_tag="VFV99_17320"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5061132.1"
                     /translation="MLLAQLVEASDQVAQVPGRLKKIELIATCLRNMGSDERAIGSRY
                     LAAQLPQKTGIGYATVSEVMKQASSAVAPWLTLGEVDRRFTAMADLRGAGSATQRKEH
                     LGALLALATLQEQKFLAGLVVGEVRHGALDGIVVEAIAKAAELPADSVRRAYMLAGDL
                     GEVAAAALEGGAAEIGRFGLSLFRPILPMLAQTADDPASALASFDGPVALEHKLDGFR
                     VQIHKDGDIVRAYSRALNEVTEYVPEVIEAARTMPARKLILDAEAIVLAAGGRPLPFQ
                     DTMKRFGKKGVELKAKMPLTVSVFDALLVDDDTLIAEPAKRRFEVVGAVAPQQAVKRL
                     VTNDVYEASTFYAQAIAGGHEGVMAKALDAPYDAGNRGASWLKIKKIHRLDLVVLAAE
                     WGSGRRKGWLSNIHLGARDPASGELIMLGKTFKGMTDEMLKWQTAEFLAREVDRDGHI
                     VFVRPELVVEIAFNDVQRSPRYPAGLALRLARVVRYRNDKTAAEADTIDAVRAIAITD
                     GVLPA"
     gene            complement(7447..7986)
                     /locus_tag="VFV99_17325"
     CDS             complement(7447..7986)
                     /locus_tag="VFV99_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061133.1"
                     /translation="MRTLAALDACLDHVRAAPRLAGTIELIVRRPTKEARDIVEEAEL
                     DVSRGLVGDNWLERGSKRTPDGSANPEQQLTLMSVRAIAALADRDRWPLAGDQFFVDM
                     DLSATNLPAGTQLQLGTALVEISATPHTGCDKFTARFGSDATKWVNSGVGRELRLRGV
                     NARIVRGGIARRGDSLRRV"
     gene            complement(8049..9071)
                     /locus_tag="VFV99_17330"
     CDS             complement(8049..9071)
                     /locus_tag="VFV99_17330"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX5061134.1"
                     /translation="MPLDPMRERLIRALPKVELHLHIEGTLEPEMMFSLAAKHGVALP
                     YASVDAVRAAYNFSDLQSFLDLYYAGCAVLRDRQDFYALAMAYFTRAHADRVVHAELF
                     FDPQTHTARGVPMEVVIGGLRDAMADAHKRYDISSQLIMCFLRHLSEEDAFKTLAEVM
                     PFKGEIVGVGLDSGEQGNPPSKFERVFKQAREYGLRTVAHAGEEGPAAYIREALDLLF
                     VERIDHGVRCEEDKALVDELIKKQIPLTVCPLSNLKLRVVKDLRDHNFARMLRKGVLV
                     TINSDDPAYFGGYIGENYRQTAAALDLTAKEIATAAENAVHASFMPSAQKLSLTSAIQ
                     LEAQRT"
     gene            9100..9900
                     /locus_tag="VFV99_17335"
     CDS             9100..9900
                     /locus_tag="VFV99_17335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061135.1"
                     /translation="MGSEVTRFVGSIPALYDRHLGPVLFEPYAHDLAQRVPKGAQTLL
                     EIAAGTGRVTRQLLAVLPSGAVAVVTDLNEPMLDEAKQHISDPRVRWQAADAMALPFA
                     DGAFDVVACQFGLMFVPDKLVALREMRRALRPGGTLLVSVWDAIENNPATAVLHKLAF
                     ETFPNDPPMFMKTPFSMPDAAALAALAREAGFSDVRVETVAKTGEAESAAHLATGFVR
                     GNPLWNQLVDRGVDAPAFEAAVAAALTREFGGAPCRSALSAHVLTAVA"
     gene            9963..>12606
                     /locus_tag="VFV99_17340"
     CDS             9963..>12606
                     /locus_tag="VFV99_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEX5061136.1"
                     /translation="MTEDVATPETPAFKLDFDPVPALVQTLNLPAKGVAAVIALLEEG
                     NTVPFIARYRKERTGGLDEVQIRAIEEKREYLIELESRRAAILASVAEQGKLTPEFEQ
                     KLRAATGKAELEDLYAPYRPRRKTRASVARERGLEPLAQKILAQGAEGDPAAEAAAFV
                     TDQVANADDALQGARDIVAETIADTAEVRAFVRREFAEHGKLSSIVVPGKGDEPTKFE
                     QYYQFEETVKTIPSHRFLAIRRGEAEGVLRAHVAVDTAKVEQGIQRLANLVEASPWAE
                     QMRLAISDALKRLLAPSVENDVRAELKTRSDSAAVDIFAGNLRNLLLAAPLGSASVIG
                     VDPGLRTGCKCAAIDATGKFLGTITIYISQGDAQLAKAKDDFVAFVREHKPRAIAVGN
                     GTGGREAEAFVKKTLADAGMREGAEAPFIVQVNEAGASVYSASDLAREEFPELDLTIR
                     GAISIARRLQDPLAELVKIEPKSIGVGQYQHDVHQTLLGKKLGDVVESCVNHVGVELN
                     TASAQLLGYIAGIGKSLAKKIVLHRDANGAFASRAQLQDVSGLGPKAFEQAAGFLRIH
                     GAGNPLDGSAVHPERYPLVEQMAGDLGVPLPELIGNQELVDKIDLSKYVQGDVGEPTL
                     RDIVAELGKPGRDPRAEFAPPKFRDDVTTMEDLKPGMVLEGVVTNVTAFGAFVDIGVH
                     NDGLVHVSQLAEKFVKDPSEVVKVGQKLTVRVLEVDNQRKRIALSARPPAPRREGGPR
                     QQRGPRDQQQAQGGGGGGGPRGQGRGGQGQGPGQGRGGRGPRRDDRGPRDEQRTEGAP
                     QEQLAGGSQPQRTDQGGPRGEQGGPREQRFDQGPRDQGGGGGGGRGPRRDDRGPRRDD
                     RGPRDQRFDHGGGGGGRGPRRDDRG"
BASE COUNT         2185 a         4012 c         4389 g         2020 t
ORIGIN      
        1 gcgtcgtcgc gacgcgaatg accgagatcc ctggtccgat ccgactttgt tatgagggcg
       61 cggtcacccg gctcgcgggc gaagcggggc agcgcgagat cctgcaagcg ggcatcgagc
      121 tcgtcgacgc acccgagccc gacggtgatg ccgaagtgct cgcggtcgcc gccgctgcac
      181 tcgcgcgcat cgcgctgccc gagatccgga tcgatatcgg ccacgtcgcg cccgctgcat
      241 tcgtgctcgg cagtgcgccg gatccgcatg cgcgcgcgct gatcacgaac tcgctcgcgc
      301 gcaaagatcg agcgggactg cgcgccgctg cgcgcgtgtt gcccgaaggc gtcgcgccac
      361 tcgccgaggc actcgccacg ttgtggggac ctgcggatgc gacgctcgat cgcgcggccg
      421 cgttgccgtg gcccgcaccg gtgaaagcgg cactcgatca gttgcgcgcg gtgctcgctg
      481 cgtttgcgga cctcgccgaa gcaccgccgc cggtcacgat cgatctcggc gacttgcgcg
      541 gcttcgatta ctacaccggc gtgcgcttcg cggcgtatgc gggcggcgcg cccgacgcgg
      601 tgttgcgcgg cggccgttat gacgagttgc tcgcgcgcta tggccggaag gcgcaggcga
      661 cgggctttgc gatcgatctc gaggccgttg cgcaagcgca acgatcgatc gggatcgcgc
      721 caccgactgc cgcgcttggc gtcgcgacac acggtgcggg agcaggcgag ctggcgcgtg
      781 tgcttcggcg cgccggacta cgcgcggtta cgcaaagccg cgctcccgcg agttggggcg
      841 catggttgcg cggagcgggc ctcgaggccg tactcatcgg agatcagctg atcgggatcg
      901 acgacaaccc gcgttcagcg aaggctgcga tagaagcggc ccgagcgggc gacacaacgg
      961 cgctgatcgc gctaataaag gcgacgtaga tgtcggtcgt catcgtcgtc ggcgcccaat
     1021 ggggagatga aggcaaggga aaggtcgtag acctgttcac cgaacgcgct gacgtggtcg
     1081 tgcgctacgg tggcggcgcg aacgccggtc acacgctcgt gatcgatggc aagaagctcg
     1141 tcacgcatct ggtgccgagc ggcgtctgcc atccgggcaa gagctgcgtg ctcggcgatg
     1201 gcatggtgat cgatccgcac acgctgctcg aagagatcag cgagtgccaa gcgcgcgggc
     1261 ttctgtgtca gcaggagctg ctgatcggcc tcggcgcgca cgtgatcctg ccgtaccaca
     1321 agctgatcga cggcctgcgc gaagacttcg gtgcgaagcg cggcaaagcg atcggcacca
     1381 cgcggcgcgg gattggcccg gcgtacgaga tgaaagcagc gcgccgcggc gtgcgcatgc
     1441 gcgacttgct ccggcccgag cggctgcgtg cacttgttgc cgcaaacctc gacgatctcg
     1501 cgcccgtgat cacgcatctc ggcggcgcgg tgccgagcac cgccgaagtc ggcacgtgga
     1561 tcgacgacgc actcgccgcg ggcgagaagc tgcgcgcgta taccggcgat gctggtcgtc
     1621 atgtcgcgcg tgcgatcgca gagggccgtc acgtgttgtt cgaaggcgcg cagggctgct
     1681 tgctcgatct cgatcacggt acgtatccgt acgtgacgtc gtcatcgacg atcgcggcag
     1741 gcgcatgtca gagcgcaggc atcggtccta cgcagatcga cgcagtcgtc ggcatcacga
     1801 aggcgtatgc gacgcgcgtc ggcgaaggac cgttcccgac cgagcttcac ggcgaggctg
     1861 gcgagcgact gcgcaaggcg ggtggcgagt ttggtgcgac aaccggtcgc ccgcgccgct
     1921 gcggctggct cgatttgccg gcgttgcgca tgggcgtgcg cctttcgggt atgcagtcgc
     1981 tcgcgctgac caagctcgac gttctggcgg gcttacctga ggtgtcggtg tgcgtgtcct
     2041 acagcctcga tggcaaggag ctcgacgagc caccgacgga tcctgaagat attgcgcgcg
     2101 cgcagccggt ctataagcac ttcaagggct gggacaagct gcccagcgag gctcacgaga
     2161 ttggtgacct gcctgcgcat gcacgcgcct acgttcagac gatctcggag atggccggag
     2221 tgccgttctg cttggtctcc gtaggcccgg atcgaagtga aacgatccgg ctcacagacc
     2281 cgttcgccgg ctaaggccgg gcaacgattt gtgatacgat tttttttcca agggacggtt
     2341 cacttggggg tcgtaggacg tgaaccgcga atacagggat acccatgaag atcgtatgtg
     2401 acgcttgcca agccaagtac tcgatctctg acgacaaggt ccaaggcaag gtcttcaaga
     2461 ttcgctgcaa gaagtgcagc aacatcatcg tcgttcgcgg cggtgctggc gcgagtgaac
     2521 cggctcctgc tccgcaggaa aaagacaccc gcgtttatga ctacggttac gacggcgccg
     2581 gcggtgcacc cgcagcagac gacgctgtct ggcacctcgt gatcaaccag gaccaagtcg
     2641 gtccgctcac cgttgccgaa gtgcagcagc gcttcgcatc gggcgaggtt gatggcgaga
     2701 cgttcacgtg gcgcgaaggc ttcgcggact ggttgccgct ctcgcaggtc gacacgttcg
     2761 cggcgctcgt cgcgtcgggc tcgaccacga ctgccggtgg cggtggcgcg gctgcggtgt
     2821 cggcgatgtt cggcggcggc gacgacatgg gcggaacggc gcgcagcgat ccgggcgacc
     2881 tgttcgcagc ggcatcgtcg tcggccaagt cgtcgcgcgg tcacgaagat gacgacggtg
     2941 gcgatctgtt cggttcgagc aaaccggctg ccgcatcggc gcagagcgcc gcggcgactg
     3001 ccaagctccg cggcgagcgc aacgagaact cggtgttgtt ctcgctgaac aacctggcgc
     3061 agctcgcgag cgattcgccg cgccccgcac cgtcatcgtc gggtggtggt ggcggtggtt
     3121 cgtcggctgg tcacgcgacc ggcgcggcgg gtggcgaagg ctccggcctc atcgacattc
     3181 gctcgatggc gagcgcgtac atgggcggtg gcggtgcagg tgcagcgatg ggcgcgccga
     3241 agtcggcgtc ggcgatcggt tcgatcgacg acctgccggt gttcggcggc ggtggcttct
     3301 ccgagcccgc cgtgatcgtg ccgaccgcgc gcggcgcgtc gaacaacaag atgctctaca
     3361 tcatgattgc cgccgtcggc ttgcttgccg tcgtcgcgat cgtgatggtc gtgttgctgc
     3421 tcaagggcgg caagaaggac ggcgacaagg tcgcggccaa cacggcgacc gaaaagaccg
     3481 acaaggccgc cacggatccg accgagaagc cgaccgaagg ctcggcggca acaggctcgg
     3541 cggagaagcc ggcggagact ggctcggcgg cggttgccgc gacggagccg gagaagaaag
     3601 agccggagaa gaaagagccc gagaagaagg agccggagca caaggcgacg aagacgacgt
     3661 cttccacgtc gaagagctcg ggtggcagca ccacgccgaa gaccaccgtc gcgaagaccg
     3721 agaccaagac ggagaccaag ccggcgggtg gtggcggtgg tgacagcggc ggctgcgacg
     3781 aagtgtcgtg cgtgctgaac aactatgaag gcgcgtgctg cgcgaagttc aagaagggcg
     3841 gcaagccgag cggcggtggt ggtggcggcg gtggtggcgg tggcggcgcg aagagcgatc
     3901 ttcccgacag cctcgatcgc gcgatgattt cgaacggcat tgctgccgtg aaggcgcgcg
     3961 tcacgacgtg cggcgacaag tcgcccgcca agggcaaggt caaggtgaag gtgcaggtca
     4021 gcggcgacgg cagggtcacg ggcgtctcgg tcgagacgac gccggatccg gcgctcggcg
     4081 cgtgcgtgtc ggctgcggtg aacaaggcgt cgttcgccaa gacgcagcac ggcggttcgt
     4141 tcagctatcc gttcgtcttc taggaacgac cgcgattcgc gacaaaggcg gctcttttcg
     4201 agtcgctctt tgtttttcgc gtcgccggtc gccgcaacgg ctatgcgtct tgccggcacg
     4261 actggctagt ggcgttgcgc gcaggttcta acagcatgcg aaacgccaac tctgcgattg
     4321 gatccgcagc gttggatctg gggtctggtg gcatggatcg cgcagttgcc ggccgccatc
     4381 atgcgcatca gccttgtaac tgttctgacc agtcttccgc tgttcatcgc ggcgtgtgcc
     4441 ggcggcgaag atggtccgac cccgtacgac gacgactacg gcatcacgag cggcaacatc
     4501 aacgagggcg cgcccgacaa cgattcgctg cccgacgaca acaaggccga cgcgcagtat
     4561 ccggatcaat acgagctacc gaaggcagag cagtccgtgg tgaagagcca gggcagccgc
     4621 ggcgtgtgct cgatcttcgc ggcgaccgcg gtcgtcgaga acctctacct caaggctggc
     4681 atgccgacgg tcgacgtcga cttcagcgag cagtacatgc agtggtcggt gaagaaccag
     4741 gtcggcgcgt tccgcaacac ggaaggcagc aacgtcgatt cgaacctcca agcggtcgtg
     4801 cgctacggcg gcatcaagga agcggcgtgg gcgtacgagt cgttcccgtg gagcgccgcg
     4861 aatgatccgg cgtgcaccgg cggcgagaac ctgccgacga agtgctacac gaacggcgag
     4921 ccgccgcagt cggcactcga cgcgcagaag ttcaagctgc cgtcgtcgcg ctggatcaac
     4981 acgaactcga tcaaggcgca cctgaccacg aagaagaccg gcgtcggcgt cggcatgacg
     5041 ttcttctatc agtcgtggaa ccaccgccgc tcgacgctgc ccgtcgacgc ggacctgtgg
     5101 cgccagggcg tcgtcacgta cccgaacgcc gacgacaaga cggcgtcgct gatgcagcgc
     5161 gcgggtcacg cgatcgagat catcggttgg gacgacacca aggaagtcgt gatgcgtgac
     5221 aaggacggtc atcccgtcct cgacacgaac ggtcagccga agaaggagaa gggcttctgg
     5281 ctgttcaaga actcgtgggg cacggcgtcg ttcggcgtcg atcacccgta cggcgctggc
     5341 tacggctggc tgtcgatgaa gtacgtcgcg gagtacggct ctgcggtggt cgcggaagtt
     5401 ccgtcgctcg cgcccgcgcg cgaagtgtgt gacgacgcgc cgaaggccga cgaggacggc
     5461 gacggcaagg cgaactgcga cgatacggac tgcgcgatgc atccgagctg cacgtcgagc
     5521 ggcggcatgg cgcacacgta cacggcgtcg ccgaatgcgt cgattcctga caacagcccg
     5581 accggtgcat cgagcgccat caacgtcagc gacagcggca cgatcgtcga cgtcaaggtg
     5641 accaccgaca tcacgcacag ctaccgcggc gacctcaagg tcacgctcag ccacggcgcg
     5701 acgtcgcaag tgatcgtcga cggcgtcggc ggctcggcgg atgacctcaa gcagacgttc
     5761 accgttacgg gtttctcggg cgagttgtcc ggagcgtgga cgctgaaggt cgaggacacc
     5821 gcgtcgcaag acgtcggtac gctgaacggc tggacgctcg aagtcacgtc gcgctaatcg
     5881 cgctaccatc ggcggatgct gctcgcccag ctcgtcgaag ctagcgatca ggtcgcgcag
     5941 gttcctgggc ggctgaagaa gatcgagctg atcgcgacgt gtctgcgcaa catgggcagc
     6001 gacgagcgcg cgatcggttc gcgctatctc gccgcgcagc tgccgcagaa gaccggcatc
     6061 ggctacgcga ccgtcagcga agtgatgaag caggcgtcct cggcggtcgc gccgtggctg
     6121 acgcttggcg aggtcgaccg ccggttcacc gcgatggccg acctccgcgg tgcgggttcg
     6181 gcgacgcaac gcaaggaaca cctcggcgcc ttactcgcgc tcgcgacgct gcaggagcag
     6241 aagttcctcg ccgggctggt ggtcggtgaa gtacggcatg gcgcactcga tggcatcgtc
     6301 gtcgaggcga tcgcgaaagc ggccgaactg ccggccgatt cggtgcggcg cgcgtacatg
     6361 ctcgccggcg atctcggcga agtcgcggcg gcggcactcg aaggtggcgc ggcggagatc
     6421 ggaaggttcg ggctgtcgct gttccggccg atcctgccga tgctcgcaca gacggccgat
     6481 gatccggcat cggcgcttgc gagcttcgat ggtcccgtcg cgctcgagca caagctcgac
     6541 gggttccgcg tccagatcca caaggacggc gacatcgtgc gcgcgtactc gcgtgcgctc
     6601 aacgaggtca ccgagtacgt gcccgaggtg atcgaagcag cgcgcaccat gccggcgcgc
     6661 aagctgatcc tcgacgccga ggccatcgtg ctcgccgcgg gcggtaggcc gctgccgttc
     6721 caggacacga tgaagcggtt cggcaagaag ggcgtcgagc tcaaggcgaa gatgccgctg
     6781 acggtgtcgg tgttcgacgc gttgcttgtc gacgacgaca cgctgatcgc agaaccggcg
     6841 aagcgacggt tcgaggtggt cggtgcggtc gcgccgcaac aggccgtgaa gcggctcgtg
     6901 acaaacgatg tctacgaggc gtcgacgttc tacgcgcaag cgatcgccgg cggccacgaa
     6961 ggcgtgatgg cgaaggcgct cgatgcgccg tacgacgccg gcaatcgcgg tgcgtcgtgg
     7021 ctgaagatca agaagatcca tcgcctcgat ctcgtcgtgc tcgcggccga gtggggcagc
     7081 ggccgccgca aaggctggct ctcgaacatt cacctcggcg cgcgtgatcc ggcgagcggc
     7141 gagctcatca tgctcggcaa gacgttcaag ggcatgaccg acgagatgct caagtggcaa
     7201 accgctgagt tcctcgcgcg cgaagtcgat cgcgacggtc acatcgtctt cgtgcggccc
     7261 gagctcgtcg tcgagatcgc gttcaacgac gttcagcgct cgccgcgtta tcccgccggg
     7321 ctcgcgctgc gtctcgcgcg tgtcgtgcgc taccgaaacg acaagaccgc ggccgaggcg
     7381 gatacgatcg atgccgtgcg tgcgatcgca atcaccgacg gcgtgctgcc cgcgtgagcg
     7441 acgatcctag acgcggcgca gtgaatcacc gcgccgagcg atgccgccgc gaacgatgcg
     7501 cgcgttgacg ccgcgcaggc ggagctcgcg gcccacgccg ctgttcaccc acttcgtggc
     7561 gtcggaacca aaccgcgccg tgaacttgtc gcaaccggtg tgtggcgtgg cgctgatctc
     7621 gacgagtgcg gtgccgagct gcagctgcgt gccggcgggc aggttcgttg ccgacagatc
     7681 catatcgacg aagaactgat cgcccgcgag cggccaacga tcgcggtcgg caagggctgc
     7741 aatcgcacgc acgctcatca gcgtcagttg ctgttcggga ttggcgctgc catccggcgt
     7801 gcgcttgctg ccgcgctcga gccagttgtc gccgacgaga ccgcgtgaga catcgagctc
     7861 cgcttcttcg acgatgtcgc gagcttcctt tgtcggtcgc cgcacgatca attcgatcgt
     7921 ccctgcgagg cgcggcgcgg cgcgcacgtg atcgagacag gcgtcgagcg cggcaagcgt
     7981 acgcatcacc ctatgatgcc gcgagaatcc cgaccgtctc gtctgtccca gcgactaaat
     8041 ggggactgtc acgtccgctg ggcctcgagc tggatcgcgc tggtcagcga cagcttttgc
     8101 gccgacggca tgaacgaggc atggaccgcg ttctcggccg cggtcgcgat ttccttcgcg
     8161 gtcaggtcga gcgcagcggc ggtctgccgg tagttctcgc cgatgtagcc accaaagtag
     8221 gccgggtcgt cgctgttgat cgtgacgagc acgcccttgc ggagcatgcg cgcgaagttg
     8281 tggtcgcgca gatccttcac cacgcgcagc ttcaagttcg acagcggaca cacggtgagc
     8341 ggaatctgct tcttgatcag ctcgtcgacg agcgctttgt cttcttcgca gcgcacgccg
     8401 tgatcgatgc gctcgacgaa caataaatcc agtgcctcgc ggatgtacgc ggcagggcct
     8461 tcttcgccgg cgtgagcaac cgtgcgcagg ccgtactcgc gcgcttgctt gaacacgcgc
     8521 tcgaacttgc tcggcgggtt gccttgctcg ccggagtcga gaccgacgcc gacgatctcg
     8581 cccttgaacg gcatgacctc ggcgagcgtc ttgaacgcgt cctcctcgga gagatggcgc
     8641 aagaagcaca tgatgagctg cgagctgatg tcgtagcgct tgtgtgcgtc cgccatcgca
     8701 tcgcgcaggc cgccgatcac gacctccatc ggaacgccgc gcgcggtgtg cgtttgcgga
     8761 tcgaagaaca gctcggcgtg gacaacgcgg tcagcgtgag cgcgcgtgaa gtacgccatc
     8821 gcgagcgcgt agaaatcctg gcgatcgcgg agcaccgcgc aaccggcgta gtagagatcg
     8881 agaaacgact gcaggtcgct gaagttgtac gcagcgcgca cagcatcgac cgacgcgtac
     8941 ggcagcgcga cgccgtgctt cgcggcgagg ctgaacatca tctccggctc gagcgtgcct
     9001 tcgatgtgaa ggtggagctc gaccttcggc aacgcgcgaa tcagtcgttc gcgcatggga
     9061 tccaacggca tggttccaca ttatgcgata acccgagcca tgggctccga ggtgacgcgc
     9121 ttcgtcggat cgattcccgc gctgtatgac cgccatctcg ggccggtgtt gttcgagccg
     9181 tatgcccacg acctcgcaca gcgtgtgccg aagggcgcac agacgctgct cgagatcgca
     9241 gccggcacgg gcagggtgac gcgtcagctg ctcgccgtgc tgccatcggg cgcggtcgcc
     9301 gtcgtcaccg acctcaacga gccgatgctc gacgaagcga agcagcacat ttccgatccg
     9361 cgcgtgcggt ggcaagccgc cgacgcgatg gcgctgccgt tcgccgacgg tgcattcgac
     9421 gtcgtcgcat gtcagttcgg cctgatgttc gtccccgaca agctcgtcgc gttgcgcgag
     9481 atgcggcgcg ctctgcgccc gggcggcacg ctgctcgtca gcgtgtggga cgcgatcgag
     9541 aacaatcccg cgaccgcggt gctgcacaag ctcgcgttcg agacgtttcc aaatgacccg
     9601 ccgatgttca tgaagacgcc gttttcgatg ccggatgcgg ccgcgcttgc ggcactcgcg
     9661 cgtgaggcgg gtttctccga cgtgcgggtc gagactgtcg ccaagaccgg cgaggcggag
     9721 tccgccgcgc acctcgcgac cgggttcgta aggggcaatc cgctctggaa tcagctcgtc
     9781 gaccgtggcg tcgatgcacc ggcgttcgag gcggctgtgg cggcggcact gacccgcgaa
     9841 ttcggtgggg cgccgtgccg ctcggcccta tccgcgcatg tacttacggc tgttgcctga
     9901 ccgtctggaa tcccgattct tgcttgctgt aggcccccgt cgcggctagg gtcgctggcc
     9961 cgatgaccga ggacgttgcg acccccgaga cccctgcatt caagctcgat ttcgatccgg
    10021 tgccggcgct ggtccagacg ctgaacctgc ccgcaaaggg cgtcgccgcg gtcatcgcgc
    10081 tgctcgaaga aggcaacacg gttccgttca tcgcgcgcta ccgcaaggag cggaccggcg
    10141 ggctcgacga ggtccagatc cgcgcgatcg aggagaagcg cgaatatctg atcgagctcg
    10201 agtcgcgccg cgccgcgatc ctcgcgtcgg tcgccgagca gggcaagctg acgcccgagt
    10261 tcgagcagaa gctgcgcgct gcgaccggca aggccgagct cgaagatctg tacgcgccgt
    10321 atcggccgcg tcgcaagacg cgtgcatcgg tcgcgcgcga gcgcgggctc gagccgctcg
    10381 cccagaagat cctcgcgcag ggcgccgaag gcgatcctgc tgccgaggct gcggcattcg
    10441 tcaccgacca ggtcgcgaat gcagacgatg cgctgcaagg tgcgcgcgac atcgtcgccg
    10501 agacgatcgc cgacaccgcc gaagtgcgcg cgttcgtacg ccgcgagttc gccgagcacg
    10561 gcaagctgtc gtcgatcgtg gtgccgggca agggcgacga gccgacgaag ttcgagcagt
    10621 actaccagtt cgaagagacg gtcaaaacca tcccgtcgca ccgcttcctc gcgatccgcc
    10681 gtggcgaggc cgagggcgtg ctccgcgcgc acgtcgcggt cgacaccgcg aaggtcgagc
    10741 aaggcatcca gcgcctggca aatctcgtcg aagcatcgcc gtgggccgag cagatgcggc
    10801 tcgcgatcag cgatgcgctc aagcgcctgc tcgcaccgag cgtcgagaac gacgtccgcg
    10861 ccgagctcaa gacgcgctcc gattcagcgg ccgtcgatat cttcgccggc aacctgcgca
    10921 atctgctgct cgcagcgccg ctcggcagcg cgagcgtgat cggcgtcgac cccggcctgc
    10981 gcaccggctg caagtgcgcc gcgatcgacg cgaccggtaa gttcctcggc acgatcacga
    11041 tctacatctc gcaaggcgac gcgcagctcg cgaaggcgaa ggacgacttc gtcgcattcg
    11101 tgcgcgagca caaaccgcgc gcgatcgcgg tcggcaacgg caccggaggt cgcgaggctg
    11161 aagcgttcgt caagaagacg ctcgccgacg ccggcatgcg tgaaggcgct gaggcgccgt
    11221 tcatcgtcca ggtcaacgaa gccggtgcaa gcgtgtactc cgcatccgac ctcgcgcgcg
    11281 aagaattccc cgagctcgat ctgacgatcc gcggcgcgat ttcgatcgcg cgtcgtctgc
    11341 aagacccgct cgccgagctc gtgaagatcg agccgaagtc gatcggcgtc ggccagtacc
    11401 agcacgacgt gcatcagacg ctcctcggca agaagctcgg cgatgtcgtc gagtcgtgcg
    11461 tgaaccacgt cggtgtcgag ctcaatacgg cgagtgctca gttgctcggc tacatcgccg
    11521 gcatcggcaa gtcgctcgcc aagaagatcg tgctgcatcg cgacgccaac ggcgcattcg
    11581 cgtcgcgcgc acaactgcag gacgtctcgg gcctcggccc taaggcgttc gagcaggcgg
    11641 ctggcttctt gcgcatccac ggcgcgggca atccgctcga cggttcagcg gtgcaccccg
    11701 agcggtatcc actcgtcgag cagatggccg gcgatctcgg cgtgccgctc cccgagctga
    11761 tcggcaacca ggagctcgtc gacaagatcg atctctcgaa gtacgtgcag ggcgacgtcg
    11821 gcgagccgac gctgcgcgac atcgtcgccg agctcggcaa gcccggccgc gatccgcgcg
    11881 ccgagttcgc accgccgaag ttccgcgacg acgtcacgac gatggaggat ctcaagcccg
    11941 gcatggtgct cgagggcgtc gtcacgaacg tcaccgcgtt cggcgcgttc gtcgacatcg
    12001 gcgtgcacaa cgatggtctc gtccacgtca gccagctcgc cgagaagttc gtcaaggatc
    12061 cgtccgaggt cgtgaaggtc ggccagaagc tgacggtgcg cgtgctcgag gtcgacaatc
    12121 agcgcaagcg catcgcactg tcggcgcgtc cgccggcccc gaggcgtgaa ggtggtccgc
    12181 gtcagcagcg cggcccgcgc gatcagcagc aggcgcaggg cggtggtggt ggcggtggtc
    12241 cgcgtggtca aggccgtggt ggacaaggcc agggaccagg gcagggccga ggcggtcggg
    12301 gtccgcgccg cgatgatcgc ggtccgcgcg acgagcaacg caccgaagga gctccgcagg
    12361 agcaactcgc gggcggttcg cagccgcaac gcactgacca aggcgggcct cgtggcgagc
    12421 aaggcggccc acgcgagcag cgcttcgatc aaggtccgcg cgatcaaggt ggtggcggtg
    12481 gtggtggtcg tggtccgcgc cgcgatgacc gcggtccgcg ccgcgacgat cgcggcccgc
    12541 gcgatcagcg tttcgatcac ggcggcggcg gtggtggtcg tggtccgcgc cgcgatgatc
    12601 gtggcc
//
LOCUS       DASUFX010000432        16172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_209857,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000432 DASUFX010000000
VERSION     DASUFX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16172
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209857"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFV99_17345"
     CDS             <1..697
                     /locus_tag="VFV99_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX5061137.1"
                     /translation="AQGKLQIGDRIIATDGVPILAVGEWYAPDGTHYVADSLTRRVNE
                     KKGGPLTLTVVRGDKHVDVTVTPKMTYLIRDEVPWSTWTTPTYLLGIAPTVRPDVLDV
                     GLGTAVVSAFEYPVIQTKVIAGGIADIFRGKEEADPGGPKRIFDEFAKAWALGPMTGI
                     QLLMVLSVYLGMFNLLPLPALDGGRLVFLTYELVTRRRANPKIEAMVHMAGIMILGVV
                     MILVTLRDFHVFS"
     gene            766..2778
                     /gene="uvrC"
                     /locus_tag="VFV99_17350"
     CDS             766..2778
                     /gene="uvrC"
                     /locus_tag="VFV99_17350"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5061138.1"
                     /translation="MIAADTLDRIPTEPGVYLFKDARGVVIYVGKAKNLRSRVRQYFR
                     EGGDERFFIAAGFFEKAVADVETIVVNSSKEALLLENHLIKKHQPKFNVKLRDDKQYL
                     VLRLVDPKPPAMDDGATRPKRDVFPRVEVVRNIRDDGANYFGPYHSATSARETLRTLN
                     RHFQLRTCTDHVLETRGRPCLQYQIKRCSGPCAIDVPPAAYGEQVEDVKMFLAGKSVE
                     LVTRLRARMERRAEREDFEVAAVLRDSIAAVERTLAKQHIVQDDFVDQDVWGLYREAD
                     TVEVVVLFVRAGKLVGRRAFSQKDQELPDAAVIAEYVQQYYATGTYIPDEIVVGVEVE
                     DGPVLVEWLSVVRGRKVKLVEPRRGIRARLVELADRNAAASAASRKNKTEDIDAKLQK
                     VADRLGLGRPPRRIECFDIAHIQGSETVASMVTFIDGVPAKGLYRKFKLRSVDNNDFA
                     AMYEVLTRRFKRTLGDDPAWARPDLLVIDGGKGQLGMAVTALTDLGVPLGGDTGVEVI
                     GLAKERELEAGSAPDRIYRRNVKDSIPLRANSPELFVLARIRDEAHRFANAFHRDRRG
                     KATLRSELDAIPGIGATRRQRLLKHFGSVRAIRLASLDDLAKAPGMNRKAAQQVVEYF
                     TQVTAAEAEETAPRVEPVYEGDESDRTESAEGAGQPQPERGAAPEH"
     gene            complement(2722..3840)
                     /locus_tag="VFV99_17355"
     CDS             complement(2722..3840)
                     /locus_tag="VFV99_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5061139.1"
                     /translation="MRDRDDDGTTDVSGIVGAFPVNDEPTNRHATSPVRLVCVAGADL
                     GRSFRIQKMPTVIGRGHGVDVALRGADISRQHASLSMIGSEYLLEDLGSQNGTWINGV
                     HIEGKATMHVGDRVQLGTTIFVFAHHDELEDRMHQLQRLEAMGALAGGMAHDFNNALA
                     VILGSIGLLERKLPANSDLLEMIGEMKSAASSASLLARRLLRLGRTEPLEFETVLLDA
                     LVQRTAAMMRRQLQNIEISVDVGPDLAMRGSQEELQQVLVNLMLNARDAMPDGGTLRL
                     YAREVGFDRAQAATHHLPTKGDYIELVVTDTGAGMDDVTLARAFEPFFTTKPPGKGTG
                     LGLAMIHSIVRRHGGSVVAESVLGRGTTFRLWLPRTLG"
     gene            4247..4612
                     /locus_tag="VFV99_17360"
     CDS             4247..4612
                     /locus_tag="VFV99_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061140.1"
                     /translation="MKLAASMFCVAALSLAACADVETYGGGQGPGITSSKIADQEFDR
                     LVKQFDPSQVTDNAQKLGGLEPADTVCEGCVMQAYRVVDLNAGIDEVRIVKDGGVQVC
                     KIFLDQGAVIFDECGLSRL"
     gene            4687..5211
                     /locus_tag="VFV99_17365"
     CDS             4687..5211
                     /locus_tag="VFV99_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061141.1"
                     /translation="MSSFRVALLVMVVGCNSSGGSHDWVPPDIDEQQTLDRIGSAAYG
                     KLCDAFADYVHDQYRSNYLVKAACTAHALETTMDAVACGDAVNMCLDTLPPVVDQQLN
                     QILAQASCPAANIDPTLCSAPVSALTACLDALGEKVDELELSATCAAFGSPVPQDWWI
                     IDEPAECAALRSGC"
     gene            complement(5208..5930)
                     /locus_tag="VFV99_17370"
     CDS             complement(5208..5930)
                     /locus_tag="VFV99_17370"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5061142.1"
                     /translation="MAPKSRPALDPVADLSRRVAHRQTTALRTCVLRWWREQGLAEHP
                     TAVGKRIALALLEQTETDTKLAGVIVLHELLADHLRAADVPTLEALFASGALADGSVV
                     DWFAVKVLGTMLLSVRGREEVARALASWRAADNMWQRRAACVAFTALAPQGDAALPNL
                     TQLIFTLCSAVVWSPERVDQTAVGWVLRELSRAEPTRVEAFIRRHARFMSRECVRQAV
                     DKHPRHKELLAHWKRATTLRRS"
     gene            6039..8642
                     /locus_tag="VFV99_17375"
     CDS             6039..8642
                     /locus_tag="VFV99_17375"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5061143.1"
                     /translation="MSKNRKISSASGSGTDRRSEVFGVIGLGAGLFLLIAMVSLQAHA
                     LVMGPFGRSMAGLFYGIAGVCGYLLIAVAVVAAIRTLLGREPVMPVTVAAGTLLGVVS
                     LATLIHLIAPGYRVLGHGPGGALGEHLAEILRAVISTAGTALLAVIGLVVAVVIATPL
                     RMRDVLHVIWDGLCFVGGGLRTGAFAFATFWADVFRAILPEKGDRDDDDDDVDVEDED
                     VLEATDDERGPEPMIIERTDKSPIEVVELTPEKKKRKKEPQKTEIDLAVPGPAADEPG
                     TTVAPEAAVVMTAPKGKRLAKGTEAPPIVEPEPTDHAGPVIFEPKFKAQDKATMAAKE
                     KAADAERQTFIKLGEGDYQLPSIQLLNYDAKEQNSIDKSAMLELSAKLTQTLENYGVK
                     GDVVAIRPGPVVTMYEFAPAPGTRVNKIVNLTDDLALALEALRVRIVAPIPGKAAVGI
                     EVPNRTREKVFLKEILADDVFRKGRAKLPLAIGKDIEGGPSVVDLGRMPHLLVAGTTG
                     SGKSVAVNAMITSLLYHCSPEDVRMIMVDPKMLELSIYEGVPHLLLPVVTDPKKANLA
                     LRWAVEEMERRYDLLAQMGVRDIATYNEKVVKQQAKVEADKLRAAADAAAKALEEDLS
                     KVVAADSPEAADAREKVETAQHQHKLPYIVVVIDEFADLMMCAPKEVETSVARIAQKA
                     RAAGIHLILATQRPSVDVITGLIKANFPSRIAFSVASKIDSRTILDQNGAEALLGAGD
                     MLFSDRGAAPQRLHGCYVDESEIHRVVEFLKTQGRPVYNLDIVKPRDEDGENGDFGGG
                     MPSGKSDDDMYDKAVYIVTTTRNASISWVQRQLRIGYNRAARLVEEMEKQGVVSPPDH
                     TNKREVLVAAA"
     gene            complement(9265..11211)
                     /locus_tag="VFV99_17380"
     CDS             complement(9265..11211)
                     /locus_tag="VFV99_17380"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX5061144.1"
                     /translation="MPAEPDYAYDYDVGPQYVDVNTAPAGSEVPNVAVFYQQLEPYGQ
                     WYDDPDYGYVFAPQQANYIPYSNGRWVNADFGFTWVSGDPFGWATDHYGRWVWRNRWV
                     WRPDTQWGPAWVQWRVGDNVVGWAPMGYDEYEAYIPEQSWRFVQPSYVTSVNLPNFYV
                     RADFDRYVESTRPIWRYERYRDRQWVAGPSRDYWQRYRVPEHRERVDLAAIGRFDADR
                     RRQAELRAREYQRTAAWQERQRRQQAVQPIIDRRMQLAEQERVRRAAEQRQLDEQRRR
                     VTENEQRIQARERALADQQRRATDAAARERAAAEQRRLEAERRQAEEQRRKVLDDQRK
                     RVEEQRLADQRRQQATEQKLKEDQRRIEQARAAAEQRQRSAQTAQQRAEQQRQLEQQR
                     KQAAEQQRQQAAEQQRQQQEQRKQAAEQQRQQQEQQRKQAAEQQRQQQEQRKQAAEQQ
                     RQQQRQQAAEQQRQQQEQRKQAAEQQRQQQEQRRAEQQRQQQEQRKQAAEQQRQQAAE
                     QQRQQQEQRKQAAEQQRQQQEQRRAEQQRQQQERQQAAEQQRQQQEQRKQAAEQQRQQ
                     AAEQQRQQQEQRKQAAEQQRQQQEQRRAEQQRQQQEQRKRAEQQRQQQQKQRAEQARE
                     KAQQRRTKSEQQKKPTKDEQQPQQ"
     gene            complement(11503..12504)
                     /locus_tag="VFV99_17385"
     CDS             complement(11503..12504)
                     /locus_tag="VFV99_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5061145.1"
                     /translation="MKRGLSLTLLLLVGCAADGDPDFEWVAPMEEGPAPIGDAVELAV
                     ASDNPDAQSVRFTVDGAELAVCDPSQPDEDCKRDDVWRWTTVFTKTGAHVIEATLLDK
                     GGETLATESRTIYVVTELPVAEIDEELSTGELPDEPDDLGQLEAAGRGYLDADRGYHS
                     AFGGIQWRVLNQRVRLHSGTPSGSVTSVRGCMNRYGASIRRWADYYKISRASVAATAL
                     TESNCTNPSGSSDGLSSGPMQVTGSTCAALMGGSRATCKSRMHSSPDYSFRVGAKYIG
                     SSYQRNQHHRDPPKIAAAYNAGSLRRSYANRWHMVSTGNHIDRYVSWYNAYRAWESQ"
     gene            complement(12631..13104)
                     /locus_tag="VFV99_17390"
     CDS             complement(12631..13104)
                     /locus_tag="VFV99_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5061146.1"
                     /translation="MHARLTSEVRGNHLTLAGMIDEHADLARLLEHAKDKQLVIDLGG
                     VTFINSLGIRDWIRMQQAAQSAGIAIELRRVAEPVVHQLNIMPAARGVSMVTSFFAAY
                     VCDDCDQEHRMLLDVRTHGSDLAKLRAPAMKCPDCQHDMDIANPPEFYFMFLAGS"
     gene            13133..13912
                     /locus_tag="VFV99_17395"
     CDS             13133..13912
                     /locus_tag="VFV99_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5061147.1"
                     /translation="MRATWLTDIHLNFMRPLALKAFYERVRQERPEVLLVTGDIGEAD
                     SVGRFVGELGAIAPVYYVLGNHDYYRSSIQAVRDRLPAGWLPAMQPVRLTERTVMLGI
                     DGWGDARCGDLHSQIQLSDWQLIDDFAPVRYDRAERIALLEDLGTTEAFALGELLARA
                     PASDALLVLTHVPPFPEACVYAGAQSEPAWLPWFTCIATGEVLAEYARAHPQKQITVL
                     CGHSHGRSVCRPEPNLEVRTGGWAPGEDEYGNPIVQATLEL"
     gene            complement(13909..14532)
                     /locus_tag="VFV99_17400"
     CDS             complement(13909..14532)
                     /locus_tag="VFV99_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061148.1"
                     /translation="MRWLALLLALTACKTGSAQGNGDQVTYHPAGTLADDDFKPTYDK
                     SDLHNALITERTAASSAEQRVHDLEDKGDYDQVQTANADLAVRQRFIASLEACETLGQ
                     TCPPRLDEPAWTYDVEADVDPKLDAPLRFDVTSWQKVSAELAGRACACRTIACVDSMF
                     AAIDRLEKRPMPEVQSDEAASSSVTQARECLYRLRGLRRTPRAVAGD"
     gene            14674..15468
                     /locus_tag="VFV99_17405"
     CDS             14674..15468
                     /locus_tag="VFV99_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX5061149.1"
                     /translation="MIDIIGILTRTWLGVHAPAAAQHVAAAPIVMTPAAGSAATAATA
                     PNASTTVDNVQKFYAGIKQVTAQFRQTVTNSTFGSTKKSDGTVWLMKPGKMRWDYVEK
                     KKDKVQVSKSFISNGKYLYVVEHGNKQVVKKNLENDLMPVAVTFLYGKGDLKAEFDAE
                     IDKSGKYGGKGDIVLKMTPKKPSAQYKNLYLVVAPDNFRVKQSVIIDSSNNLNQFEFF
                     KPDFEKEQKESIFEFDERSVKNYRIIDADQQQQGSGAPAALPPATK"
     gene            15501..>16172
                     /locus_tag="VFV99_17410"
     CDS             15501..>16172
                     /locus_tag="VFV99_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5061150.1"
                     /translation="MDYVQANGLRFAYLSAGSGPLVLLVHGFPDTAHTWDHALPKIAA
                     AGYRAVAPFTRGYHPTEIPAAGAYDSDTLGRDVLALIEALGEQQAIVIGHDWGASAAY
                     AAAGLAPERVKLLVTLAVPHPKSLKPTPKLLWTLRHFFTLRGKRAADKLRANNFAYLD
                     ELVRRWSPAWKDIPANETEAVKAAFREPGCAEAACAYYAALGARLPASQRKPIGVPTV
                     SFAGEH"
BASE COUNT         2706 a         5354 c         5294 g         2818 t
ORIGIN      
        1 cgcccagggc aagctgcaga tcggcgaccg catcatcgcc accgacggcg tgccgatcct
       61 cgctgttggc gaatggtacg cgcccgacgg cacacactac gtcgcggact cgctcacccg
      121 tcgcgtcaac gagaagaaag gcggtccgct cacgctgacc gtcgtccgcg gcgacaagca
      181 tgtcgacgtc acggtgacgc cgaagatgac gtacctcatc cgtgacgagg tgccgtggtc
      241 gacctggacc accccaacgt acttgctcgg catcgcgccg accgtccgcc ccgacgtgct
      301 agacgtcggg ctcggcaccg ccgtggtgag cgcgttcgag taccccgtca ttcagacgaa
      361 ggtgatcgcc ggcgggatcg cagacatctt ccgcggtaaa gaagaggccg accccggtgg
      421 gcccaagcgc atcttcgacg agttcgcgaa ggcgtgggcg ctcggtccga tgacgggcat
      481 ccagctgctc atggtgctgt cggtctatct cgggatgttc aacctactgc cactaccggc
      541 actcgatggc ggccgactcg tgttccttac gtacgagctc gtcacgcgcc gccgcgccaa
      601 tccgaagatc gaggccatgg tccacatggc cggcatcatg atcctcggcg tcgtgatgat
      661 cctggtgacg ctgcgcgact tccacgtgtt ctcgtagcga ccacaggtag tcaggtttcg
      721 ccggggtcgc gggccacgcc tgcgtagccg cgctaaggtc ggcccgtgat cgccgccgac
      781 accctggatc ggattcctac cgagccgggc gtctacttgt tcaaggacgc ccgcggcgtc
      841 gtgatctatg tcggcaaggc gaagaacctc cgctcgcgtg tgcggcagta cttccgcgaa
      901 ggcggcgacg agcgcttctt catcgccgcc gggttcttcg agaaggcggt cgccgacgtc
      961 gaaacgatcg tcgtcaattc ctcgaaggag gcactgctcc tcgagaacca cctgatcaag
     1021 aagcatcagc cgaagttcaa cgtcaagcta cgcgacgaca agcagtacct cgtgctgcgc
     1081 ctcgtcgatc cgaagccacc ggcgatggac gatggcgcca cgcggccgaa gcgcgacgtg
     1141 ttcccgcgcg tcgaggtcgt ccgcaacatc cgcgacgacg gcgcgaacta cttcggcccg
     1201 tatcactcgg cgacatccgc gcgcgagacg ctgcgcaccc tcaaccgtca cttccagctt
     1261 cgcacctgta ccgatcacgt gctcgagacg cgcggccgac cgtgcctgca atatcagatc
     1321 aagcgctgct cggggccgtg tgcgatcgac gtgccgccgg ctgcgtacgg cgaacaggtc
     1381 gaggacgtga agatgttcct cgccggcaag agcgtcgagc tggtgacgcg gctccgcgca
     1441 cgtatggagc gccgcgccga gcgcgaggac ttcgaggtcg cagcggtgct gcgtgattcg
     1501 atcgccgcgg tcgagcgcac gcttgcgaag cagcacatcg tgcaggacga cttcgtcgat
     1561 caggacgtgt ggggcctgta tcgcgaagcc gataccgtcg aggtcgtcgt gctgttcgtg
     1621 cgcgcaggca agctcgtcgg ccgtcgcgcg ttcagccaga aggatcagga gctgcctgac
     1681 gcggcggtga tcgccgagta cgtgcagcag tactacgcca ctggcacgta catcccggac
     1741 gaaatcgtgg tcggcgtcga agtcgaggac ggcccggtgc tcgtcgagtg gctatccgtc
     1801 gtgcgcggca ggaaagtgaa gctcgtcgaa ccgcgacgcg gcattcgtgc gcgcctcgtc
     1861 gagctcgcgg atcgcaacgc ggcggcttcg gctgcatcgc gcaagaataa gaccgaagac
     1921 atcgatgcga agctgcagaa ggtcgccgat cggctcggcc tcgggcggcc accgcgtcgc
     1981 atcgagtgct tcgatatcgc gcacattcaa ggcagcgaga ccgtcgcgtc gatggtgacg
     2041 ttcatcgacg gcgtgccggc gaagggcctc tatcgcaagt tcaagctgcg ctcggtcgac
     2101 aacaacgact tcgctgcaat gtacgaagtg ctgacgcgcc ggttcaagcg cacgctcggc
     2161 gatgatccgg cgtgggcgcg ccccgacttg ctcgtcatcg acggtggcaa aggtcagctc
     2221 ggcatggcgg tcaccgcgct caccgacctc ggcgtgccgc tcggcggcga caccggtgtc
     2281 gaggtgatcg gtctcgcgaa agagcgcgag ctcgaagccg gcagcgcgcc ggatcgcatc
     2341 taccgccgca acgtgaagga ctcgattccg ttgcgcgcga actcgcccga gctgttcgtg
     2401 ctggcgcgga ttcgcgacga agcgcaccga ttcgcgaacg cgttccaccg cgatcgccgc
     2461 ggcaaggcga cgttgcgctc cgagctcgac gcgattcctg gcatcggcgc gacgcggcgc
     2521 cagcgcctgc tcaagcactt cggcagcgtg cgcgcgatcc ggcttgcatc gctggacgac
     2581 ctcgcgaagg caccggggat gaatcgcaag gcggcacagc aggtcgtcga atacttcacc
     2641 caggtgaccg ctgcggaggc ggaagagacg gctccgcgcg tcgagccggt ctacgagggc
     2701 gacgaatccg atcgcaccga atcagccgag ggtgcggggc agccacagcc ggaacgtggt
     2761 gccgcgcccg agcactgact cggcgacgac cgagccgccg tgccggcgca cgatgctgtg
     2821 aatcatcgcg aggccgagac cggtgccttt gcccggcggt ttcgtcgtga agaacggctc
     2881 gaacgcgcgt gcgagcgtga cgtcgtccat gccggcgccg gtatcggtga cgacgagctc
     2941 gatgtaatcg cccttcgtgg gcaggtgatg tgttgccgcc tgcgcgcgat cgaagccgac
     3001 ctcgcgcgcg tacaggcgga gcgtgccgcc gtctggcatc gcgtcgcgcg cgttgagcat
     3061 gaggttgacg agcacctgct gcagctcttc ttgcgagcca cgcatcgcga gatcgggacc
     3121 gacgtcgacc gagatctcga tgttctgcag ctggcggcgc atcatcgcag cggtgcgctg
     3181 gacgagcgca tcgagtagca cggtctcgaa ctcgagtggc tccgtgcggc cgagtcgcaa
     3241 cagccggcgc gcgagcagcg aggccgagct cgcggcggat ttcatctcgc cgatcatctc
     3301 gagcaggtcg ctattcgccg gcagcttgcg ctcgagcaga ccgatcgagc cgaggatcac
     3361 ggcgagcgcg ttgttgaagt cgtgtgccat gccgccagcg agcgcgccca tcgcctcaag
     3421 ccgctggagc tgatgcatgc ggtcttcgag ctcgtcgtga tgcgcgaaca cgaagatcgt
     3481 cgtgccgagc tgcactcgat cgccgacatg catcgtcgcc ttaccctcga tatgcacgcc
     3541 gttgatccat gtcccgttct ggcttccgag atcctcgagc agatattcgc tgccgatcat
     3601 cgacaggctc gcgtgctggc gcgagatgtc tgcgccacgc aacgcgacgt cgacgccgtg
     3661 cccgcgaccg atcaccgtcg gcatcttctg gatgcgaaac gaccgaccga gatcagcacc
     3721 ggcgacgcag acaagccgca cgggcgacgt cgcgtggcgg ttcgtcggct cgtcgttgac
     3781 ggggaacgcg ccgacgatgc cactgacgtc ggtggttcca tcgtcgtcgc ggtcgcgcat
     3841 caatcccacg gtatcccgtc gaagatgctc actcgggagg tcgtggggta acgctgcgtt
     3901 agttccaccc gatcgggtac tgaaaacccg aaccttcgcg gcaagcgctg atgcgcctac
     3961 atccgatgca tcgacgatcg tcgttctcga gtgggcgaga cgatcggtca ctgatcgatc
     4021 tgcatcctcg atgcgcgaac aaggcaccga gatcgatcac ggcggcatca agtgctgtcg
     4081 cagcgtcgca gccgtcgcag ccagtcgcag taatcaacgg gtcgcgcgtt gcttacttcg
     4141 tcgccaaaga ttccgatttt cgacggtgga ttttggaatt cacgaccacc atataatggt
     4201 cagtcacgcg agttgttcct gcgtgttcat gcctaggagg gttgtcgtga agctcgctgc
     4261 aagcatgttt tgtgtcgctg cgctctcgct cgccgcatgt gccgatgtcg agacctacgg
     4321 tggtggtcaa ggcccaggca tcacctcgtc gaagatcgct gaccaagaat tcgatcggct
     4381 cgtgaagcag ttcgatccgt cgcaggtgac ggacaacgcg cagaagctcg gcggcctcga
     4441 gcccgcagac accgtgtgcg agggctgcgt gatgcaggcc tatagggtcg tcgacctgaa
     4501 cgccggcatc gacgaggttc gcatcgtcaa agacggcggc gtgcaggtct gcaagatctt
     4561 cctcgaccag ggagcggtca tcttcgacga gtgcggcttg tctcgcctgt aattgccgaa
     4621 gtccgccggg ctctacgtgg cacgcgccac ggcgcgccgg tgcgatacaa tgcaacgctg
     4681 ggggcaatga gtagctttcg cgttgcatta ctcgtgatgg tggtgggctg caattcgtca
     4741 ggcggcagtc atgactgggt gccgcccgac atcgacgaac aacaaacgct cgatcgaatc
     4801 ggctccgccg cgtacggcaa gctctgcgac gcgttcgcgg actacgtgca cgaccagtat
     4861 cgatcgaact acctcgtcaa ggccgcgtgc acggcgcacg cgctcgagac gacgatggat
     4921 gcagtcgcgt gcggcgacgc ggtcaacatg tgcctcgaca cgttgccacc ggtcgtcgat
     4981 cagcagctga accagatcct cgcgcaggcg agctgtcccg ccgccaacat cgatccgacg
     5041 ctgtgctccg cgccagtcag cgcgctcacc gcgtgcctcg atgcgctcgg cgaaaaagtc
     5101 gacgagctcg agctctcggc gacgtgcgcc gcgtttggtt caccagtgcc gcaagactgg
     5161 tggatcatcg acgagcccgc cgaatgcgcg gcgctccgct cgggctgcta gctccggcga
     5221 agcgtcgtcg cgcgcttcca gtgtgcgagc agttccttgt ggcgcggatg tttgtcgacg
     5281 gcctgacgca cgcactcgcg cgacatgaac cgcgcgtggc ggcgaatgaa tgcctcgacg
     5341 cgcgtcggct ccgcgcgtga cagctcgcgg agtacccagc cgacagccgt ctgatcgacg
     5401 cgctcgggcg accacacgac tgccgagcac agcgtgaaga tcagctgcgt caggtttggc
     5461 aacgcggcat cgccttgagg tgcgagcgcg gtgaacgcga cgcacgcggc gcggcgttgc
     5521 cacatgttgt cggcagcgcg ccagctcgcg agcgcgcggg cgacttcctc gcgaccacgc
     5581 acgctgagca gcatcgtgcc gagcaccttg acggcaaacc aatcgacgac gctgccgtcg
     5641 gcgagcgcgc ccgatgcgaa tagcgcttcg agcgtgggca cgtcggccgc gcgcagatga
     5701 tccgcgagca gctcgtgcag cacgatcact cccgcgagct tggtgtccgt ctctgtctgc
     5761 tcgagcaatg cgagcgcgat gcgtttgccg accgcggtcg gatgctcggc caggccctgc
     5821 tcccgccacc agcgcagcac gcacgtgcgc aaagcggtcg tctggcgatg ggcgacgcgg
     5881 cggctcagat ccgcgaccgg atcgagagcc ggacgcgatt tgggcgccat ccttccacgg
     5941 tgcctgaaga ctactcagtg cgtctgcgta gaaaatcttg ggccgtcatg tgctcccatg
     6001 taggataatg tgtcccaggg agttttgttc ggagacccat gtccaagaac cgaaagattt
     6061 cgagcgcatc ggggagcggg acggatcggc gcagcgaggt gtttggcgtg atcgggctcg
     6121 gggctgggtt gttcctgctc atcgccatgg tttcgctgca ggcccacgcg ctcgtgatgg
     6181 gtccgttcgg ccgttcgatg gcaggtctgt tctacggcat cgccggcgtg tgtggctact
     6241 tgttgatcgc ggtcgccgtc gtcgctgcga tccgcacgct gctcggtcgc gagccggtga
     6301 tgccggtgac ggtcgccgcg ggcacgctgc tcggtgtcgt ctcactcgcg acgctcatcc
     6361 atctcatcgc gccgggctat cgcgtgctcg gtcatggtcc gggcggtgcg ctcggtgagc
     6421 acctcgccga gatcctgcgc gcggtgatca gcacggcagg caccgcgctg ctcgccgtga
     6481 tcggcctcgt cgtcgcggtc gtgatcgcga cgccactgcg catgcgcgac gtgctgcacg
     6541 tgatctggga cggcctctgc tttgtcggcg ggggcctgcg caccggcgcg ttcgcgttcg
     6601 caacgttctg ggccgatgtg ttccgcgcga tcctccccga gaagggggac cgcgacgatg
     6661 atgacgacga cgtcgacgtc gaagacgaag acgtgctcga agcgaccgac gacgaacgcg
     6721 gccccgagcc gatgatcatc gagcggaccg acaagtcgcc gatcgaagtc gtcgagctca
     6781 cgcccgagaa gaagaagcgc aagaaggagc cgcagaagac cgaaatcgat ctcgccgtcc
     6841 ccggcccggc cgccgacgag cccggcacga cagtcgcgcc cgaagccgca gtcgtcatga
     6901 cggcgcccaa gggcaagcgc ctcgcgaagg gcaccgaagc gccgccgatc gtcgaacccg
     6961 agccgaccga tcatgccggc ccggtgatct tcgagccgaa gttcaaggcg caggacaagg
     7021 cgacgatggc cgcgaaggag aaggcggccg acgccgagcg ccagacgttc atcaagctcg
     7081 gcgagggcga ctaccagttg ccgtcgatcc agctgctcaa ctacgacgcg aaggagcaga
     7141 actcgatcga caagtccgcg atgctcgagc tatccgcgaa gctcacccag acgctcgaga
     7201 actacggcgt caagggtgac gtcgtcgcga tccgcccggg ccccgtcgtc acgatgtacg
     7261 agttcgcgcc ggcgccgggc acgcgcgtca acaagatcgt caacctcacc gacgacctcg
     7321 cgctcgcgct cgaagcgctg cgcgtgcgca tcgtcgcgcc gattcccggc aaggccgctg
     7381 tcggcatcga ggttccgaac cgcacgcgcg agaaggtctt cctcaaggag atcctcgccg
     7441 acgacgtgtt ccgtaaaggc cgcgccaagc tgccgctcgc gatcggcaag gacatcgagg
     7501 gcggcccgag cgtcgtcgat ctcggccgca tgccacacct gctcgtcgcc ggtacgacgg
     7561 gatcgggcaa gtcggtcgcc gtcaacgcga tgatcacgag cctgctctat cactgctcgc
     7621 ccgaagacgt ccgcatgatc atggtcgatc cgaagatgct cgagctctcg atctacgagg
     7681 gtgtgccgca tctgctcctc ccggtcgtca ccgatccgaa gaaggcgaac ctggcgctgc
     7741 gctgggccgt cgaagaaatg gagcgccgct acgatctgct agcgcagatg ggcgtgcgcg
     7801 acatcgcgac gtacaacgaa aaggtcgtca agcagcaggc gaaggtcgaa gccgacaagc
     7861 tgcgcgcggc cgccgatgcc gctgcaaagg cgctcgaaga ggatctctcg aaggtcgtcg
     7921 ccgccgactc acccgaagca gccgacgcgc gcgagaaggt cgagaccgcg cagcaccagc
     7981 acaagctgcc gtacatcgtt gtcgtgatcg acgagttcgc cgacctcatg atgtgcgcgc
     8041 cgaaagaagt cgagaccagc gtcgcccgca tcgcgcagaa ggcgcgcgcc gccggtatcc
     8101 acctcatcct cgcgacgcag cgtccgagcg tcgacgtcat caccggcctc atcaaggcga
     8161 acttcccgtc gcgtatcgcg ttctcggtcg cgtcgaagat cgattcgcgc acgattctcg
     8221 accagaacgg tgccgaagca ctgctcggtg ctggcgacat gttgttctcg gatcgcggcg
     8281 cggcgccgca gcgtctgcat ggctgctacg tcgacgagag cgagatccat cgcgtggtcg
     8341 agttcctcaa gacgcagggc cgcccggtct acaacctcga catcgtgaag ccgcgtgacg
     8401 aggacggcga gaacggcgac ttcggcggcg gcatgccgag cggcaaatcc gacgacgata
     8461 tgtacgacaa ggccgtttac atcgtgacga cgacgcgcaa cgcgtcgatc tcgtgggtgc
     8521 agcgccagct ccgcattggc tacaaccgcg ccgcgcgtct cgtggaagag atggagaagc
     8581 agggcgtggt gagcccgccc gaccacacga acaagcgcga agtgctcgtc gctgctgcgt
     8641 agccccgacg cacgagtgcc cactcacgcg tgagccgctt gcgagctctc tcgcacgtca
     8701 ggcgcggcga gctctctcgc acgtcacgcg cgttccgcgc caccgctcgg cacagcgcga
     8761 acacgcgagc tgcacgccgc ggtggggcac tcgcgccgcc gcatggaacg gagtgctcgc
     8821 accacccacc ggcgcggcgc tccatgtcgt tccggcgcat gactgggccg cctccccgct
     8881 acggcgcttc gccaccccac ctgcggcatg catctcgcgt tgacgagctg ccgagatcgc
     8941 gccgcgcgcc ggaggcgctt gcgaacccac tttcacggct cgcgcgttcg gcgcaccgct
     9001 gctgggcgcg aacacgctgt cgtcgtagtg cgcatcgatc gctgcggcaa gctccgcgat
     9061 gctgcgctga tgccgcgcca agtaacggct tgccgcaacg ccgcgccgat cgcgacgctg
     9121 cgctgatact gcgtcgaagc ggtagtacca gccgatacac gttgccgcga cgtcgcgctg
     9181 atctcgacgc ttgcgctcat gccgcaccga aaccgattgc cgcgacgccg cgctcatcgc
     9241 gacgccgcgg cgagtcgact cgctctactg ctgcggctgc tgctcgtcct tggtgggctt
     9301 cttctgctgc tcactcttgg tacggcgctg ctgggccttc tcgcgtgcct gctcggcacg
     9361 ctgtttctgc tgctgctgac gctgctgctc ggcacgcttg cgctgctcct gctgctgccg
     9421 ctgctgctcg gcgcggcgct gctcttgctg ctggcgttgt tgctcagccg cctgcttccg
     9481 ctgctcctgc tgctggcgct gctgctcggc tgcttgctgg cgttgctgct cagctgcctg
     9541 tttccgctgt tcctgctgct gacgctgctg ctccgcggcc tgctggcgct cttgctgctg
     9601 gcgttgttgc tcggcgcggc gctgctcttg ctgctggcgt tgttgctcag ccgcctgctt
     9661 ccgctgctcc tgctgctggc gttgctgctc agcggcctgc tggcgttgct gctcagcggc
     9721 ctgcttccgc tgctcctgct gctgacgctg ctgttcagcg cggcgctgct cttgctgttg
     9781 gcgttgctgt tcggccgctt gcttgcgctg ctcctgctgc tggcgctgct gctccgctgc
     9841 ttgctggcgt tgctgctggc gctgctgctc ggccgcttgc ttccgctgct cctgctgctg
     9901 gcgctgctgc tccgcggctt gcttgcgttg ctgctcctgc tgctggcgct gctgctctgc
     9961 agcctgcttc cgctgctctt gctgctgacg ctgttgctcg gcggcctgct ggcgctgctg
    10021 ctctgcggcc tgcttgcgct gttgctccag ttgccgctgc tgctccgcac gctgctgtgc
    10081 ggtttgcgcc gagcgctggc gctgctcggc ggctgcgcgc gcttgctcga tccgacgctg
    10141 atcctccttt agcttctgct cggtggcctg ctggcggcgt tggtccgcga gccgctgctc
    10201 ctcaacgcgc ttgcgctgat cgtcgagcac cttgcggcgt tgctcttcgg cctggcgacg
    10261 ctcggcttcg agtcggcgtt gctctgcggc cgcacgctcg cgcgctgctg cgtcggttgc
    10321 gcggcgctgc tggtcggcga gtgcgcgttc acgcgcttgg atgcgctgct cgttctcggt
    10381 cacgcggcgg cgttgctcgt caagttggcg ttgctcggcg gcgcggcgaa cgcgttcttg
    10441 ttcggcgagc tgcatgcggc gatcgatgat cggctgcacc gcttgctggc ggcgctggcg
    10501 ctcttgccat gcagcagtgc gctggtattc acgcgcgcgc agctcggcct ggcgccgacg
    10561 gtccgcatcg aagcggccga tcgccgcgag gtcgacgcgt tcgcgatgct cgggcacgcg
    10621 atagcgctgc cagtagtccc gactcggacc ggcgacccat tggcgatcgc gatagcgctc
    10681 gtaccgccag atcgggcgcg tcgactcgac gtaacgatcg aagtcggcgc gcacgtagaa
    10741 gttgggcaga ttcaccgacg tcacgtacga cggttgcacg aagcgccacg actgctcggg
    10801 gatgtacgcc tcgtactcgt cgtagcccat cggcgcccag ccgacgacgt tgtcaccgac
    10861 acgccactgg acccacgcgg gtccccactg cgtgtcgggt cgccagaccc agcgattgcg
    10921 ccagacccaa cgaccgtagt gatcggtcgc ccagccgaac ggatcgcccg agacccaggt
    10981 gaagccgaag tcggcattca cccagcgccc gttcgagtat ggaatgtagt tggcctgctg
    11041 cggcgcgaag acgtagccgt agtcgggatc gtcgtaccac tgaccgtacg gctcgagctg
    11101 ctggtagaag accgcgacgt tcggaacttc gctgccagct ggcgccgtgt tgacgtcgac
    11161 gtattgcgga ccgacgtcgt aatcgtaggc gtagtcgggt tcggccggca tcgccgacgc
    11221 ggatggcgcg gcaggcgacg ccgatcgtgg cgcagccgga gctggcgctg gaacctcagg
    11281 tgatggctcg gtgggcgcga gcgccccgcc gggcgcatac gcctgcgaat catagggcac
    11341 ctgcgcagcg ggaggcgaag agctcctcgt acatgcgacg aggagcgaac aagctaacca
    11401 atgttggaca cgcatagatg actccgttcg cgacaattct gcgtcggagt catgcaggcc
    11461 ctacgcctgt ctacagatag cgaaatcgcg tgcgaacaac tgctactgcg attcccacgc
    11521 gcgatacgcg ttgtaccagc tcacgtagcg gtcgatgtga tttcccgtcg acaccatgtg
    11581 ccagcggttc gcatacgacc tgcgcagcga gcccgcgttg taggcggcgg cgatcttcgg
    11641 tggatcgcga tggtgctggt tacgctggta gctcgagccg atgtacttcg caccgacgcg
    11701 gaacgagtag tcggggctcg agtgcatgcg cgacttgcag gtcgcgcgcg agccacccat
    11761 cagcgccgcg cacgtcgaac cggtgacctg catcggtccc gacgagagac cgtcggacga
    11821 gcccgacggg ttcgtgcagt tcgattcggt gagcgccgtc gcggcgaccg acgcgcgaga
    11881 gatcttgtag tagtcggccc acctgcggat cgacgcgccg tagcggttca tgcacccacg
    11941 caccgacgtg accgagccgg acggcgtgcc gctgtgcaga cgcacacgct ggtttaggac
    12001 gcgccactga atgccgccga aggcgctgtg atagccgcga tcggcgtcga ggtaaccgcg
    12061 accagctgct tcgagctgac cgaggtcatc gggctcatcc ggcagctcgc cggtcgacag
    12121 ctcctcgtcg atctcggcga ccggcagctc ggtgacgacg tagatcgtgc ggctctcggt
    12181 cgcgagcgtc tcgccgcctt tgtcgagcag tgtcgcctcg atcacgtgcg cgccggtttt
    12241 ggtgaagacg gtcgtccacc gccacacgtc atcgcgcttg cagtcctcgt cgggctgcga
    12301 aggatcgcac accgcgagct ccgcgccgtc gaccgtgaac cgcaccgatt gggcatcggg
    12361 attgtcgctc gcgacggcaa gctccaccgc atcgccgatc ggcgccggtc cctcctccat
    12421 cggcgcgacc cattcgaaat ccgggtcgcc atcagcggcg caaccaacca ataacaagag
    12481 cgtgagtgaa agtccccgtt tcatcaacgc gccgctcgag cacgtggcgt gccgatcatg
    12541 cggcttgtaa gcgtggagaa tgtgctgtgc gtgaatacga cttgcgcatc acaggttgcg
    12601 accgatcggt tgcggtcggt cgcgctgtcc tcagctgccg gcgaggaaca tgaaatagaa
    12661 ctcgggcgga ttcgcgatgt ccatgtcgtg ctggcagtcg gggcacttca tcgctggcgc
    12721 gcgcagcttc gcgagatccg aaccgtgcgt gcggacatcg agcagcatgc ggtgttcttg
    12781 atcgcagtcg tcgcacacgt acgccgcgaa gaacgacgtc accatcgaca cgccccgcgc
    12841 tgcgggcatg atgttcagct ggtgcacgac aggttcggcg acgcggcgaa gctcgatcgc
    12901 gatacctgca ctctgcgcgg cctgctgcat gcggatccag tcgcggatgc cgagcgagtt
    12961 gatgaacgtg accccgccga gatcgataac gagctgcttg tccttggcgt gctcgagcag
    13021 acgagcgagg tccgcgtgct cgtcgatcat accggcgagc gtcaggtgat tccccctgac
    13081 ctcggaggtc aaccgcgcat gcatgtggag atcttacgct aggctggagg gcgtgcgggc
    13141 cacttggctc actgacattc acctgaattt catgcgccct ctggcgctga aggcgttcta
    13201 cgaacgggta cgtcaggagc gccccgaggt cctgctcgtg accggagaca tcggggaggc
    13261 ggactcggtg gggcggttcg tcggcgagct cggcgcgatc gcgcccgtct attacgtgct
    13321 cggcaaccac gactactacc ggtcgagcat ccaggccgtg cgcgatcgcc tacccgccgg
    13381 ctggctgccc gcgatgcagc cggtccggct caccgagcgc accgtcatgc tcggcatcga
    13441 cggctggggc gacgcacgct gcggcgattt gcactcgcag atccagctct cggactggca
    13501 gctgatcgac gacttcgcgc cggtccgcta cgaccgtgcc gaacgcatcg cgttgctcga
    13561 ggacctcggc actaccgaag cgtttgcgct cggcgagcta cttgcgcgcg cacctgcaag
    13621 cgacgcactg ctcgtgctca cccacgtgcc accgttcccg gaagcatgcg tctacgcggg
    13681 cgcacaatcg gagccggcgt ggttgccatg gttcacatgc atcgcgaccg gcgaggtgct
    13741 cgcggagtac gcgcgcgccc acccgcagaa gcagatcacg gtgctgtgcg gccactcgca
    13801 cggcaggagc gtgtgccgac ccgagccaaa cctcgaggtg cgcaccggcg gctgggcgcc
    13861 tggcgaggac gagtacggca atccgatcgt ccaggcgacg ctcgagctct aatcgccggc
    13921 gacggctcgc ggcgtacgcc gcaatccacg caaccggtac aagcactcgc gcgcctgcgt
    13981 gaccgagctg ctcgcagctt cgtcgctctg cacctcgggc atcggtcgct tctcgagccg
    14041 atcgatcgca gcgaacatcg aatcgacaca cgcgatcgtg cggcacgcgc acgcgcgacc
    14101 ggccagctcg gcagacacct tctgccagct cgtgacgtcg aagcgcagcg gcgcatcgag
    14161 cttcggatcg acatcggctt cgacatcgta cgtccacgcc ggttcgtcga gtcgcggcgg
    14221 gcacgtctgg ccgagtgtct cgcacgcctc gagcgacgcg atgaagcgct ggcgcaccgc
    14281 gagatcagcg ttggcggtct gtacctgatc gtagtcgccc ttgtcctcga gatcgtggac
    14341 gcgctgctct gcggagctcg cggcagtgcg ctctgtgatc aaggcattgt gcaggtccga
    14401 cttgtcgtag gtcggcttga agtcgtcgtc ggcaagggtt ccggccgggt gataggtcac
    14461 ctggtcgcca ttcccctgag cgctcccggt cttgcatgca gtaagtgcca gcaagagtgc
    14521 gagccatctc atcccgacta aggtaactga gcacaagccg tgctaccacc atgcaactgc
    14581 acgagactca tggtttctca tccatgaatc gccagcaacc ccacgcgtca aaacaccgtg
    14641 gcgcttgttt ggcgcccttc ggagcctgaa tcaatgatcg acatcatcgg aatcctcact
    14701 cgcacgtggt tgggcgtgca tgcaccggca gctgcgcagc acgtcgcggc ggcgccgatc
    14761 gtcatgacgc ccgcggcagg ttcggcggcg acggcagcca ccgcgccgaa cgcgtcgacc
    14821 acggtcgata acgtccagaa gttctacgcg ggcatcaagc aggtcaccgc gcaattccgc
    14881 cagaccgtca cgaacagcac gttcggctcg accaagaaga gcgacggcac ggtgtggctg
    14941 atgaagcccg gcaagatgcg ctgggactac gtcgagaaga agaaggacaa ggtccaggtc
    15001 tcgaagagct tcatctcgaa cggcaagtac ctctatgtcg tcgagcacgg caacaagcag
    15061 gtcgtgaaga agaacctcga gaacgacttg atgccggtcg cggtcacgtt cctctatggc
    15121 aagggcgacc tcaaggcaga gttcgacgcc gagatcgaca agagcggcaa gtacggcggc
    15181 aaaggcgaca tcgtgctgaa gatgacgccg aagaaaccgt cggcgcagta caagaacctc
    15241 tacctcgtcg tcgcgccgga taacttccgc gtcaagcagt cggtcatcat cgactcgtcg
    15301 aacaacctga accagttcga gttcttcaag ccggacttcg agaaggaaca gaaggaatcg
    15361 atcttcgagt tcgacgagcg cagcgtgaag aactaccgca tcatcgatgc ggaccagcag
    15421 caacaaggca gcggcgcccc cgccgcactg ccgccggcga cgaagtagcg cacccgctcg
    15481 cgcgcaggta acgtcgcgcg gtggattacg tccaggcgaa cggcctgcgc ttcgcgtacc
    15541 tgagcgcggg cagtggcccg ctcgtgctgc tcgtgcacgg cttcccggac accgcgcaca
    15601 cgtgggacca cgcgttgccg aagatcgcgg ctgcgggcta ccgcgcggtc gcaccgttca
    15661 cgcgcggcta tcatccgacc gagattccgg ccgcgggcgc ctacgatagc gacacgctcg
    15721 gccgcgacgt gctcgcattg atcgaagcac tcggcgagca gcaagcgatc gtgatcggcc
    15781 acgattgggg cgcgagcgct gcgtacgcag cggcaggtct cgcgccggag cgcgtgaagc
    15841 tgctcgtcac gctcgcggtt ccgcatccga agtcactcaa gccaacgccg aagctcctgt
    15901 ggacgttgcg ccatttcttc acactgcgtg gcaagcgcgc cgccgacaag ctgcgcgcga
    15961 acaacttcgc gtatctcgac gagctcgtgc gccggtggtc gccagcgtgg aaggacatcc
    16021 cggcgaacga gaccgaagcg gtgaaggccg cgtttcgcga acccggttgc gccgaagcag
    16081 cgtgcgcgta ctacgcggcg ctcggcgcgc ggttgcccgc atcacagcgg aaaccgatcg
    16141 gcgtgccgac cgtctcgttc gccggcgagc ac
//
LOCUS       DASUFX010000433         5328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_903927,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000433 DASUFX010000000
VERSION     DASUFX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903927"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFV99_17415"
     CDS             complement(<1..411)
                     /locus_tag="VFV99_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061151.1"
                     /translation="MKPRDIDLMALADGEADERELEELLRDPASRRKVEAVDELGELV
                     RGHLELSADAVPQKKLDALWREIDKGIDRERETVSKPAADVAAAPAGGWLRRVGRWFD
                     HYRGHVITGAVSAGAVAALALVLRGGADGQTTRTK"
     gene            complement(408..1091)
                     /locus_tag="VFV99_17420"
     CDS             complement(408..1091)
                     /locus_tag="VFV99_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061152.1"
                     /translation="MNKEGQRASTGRDPVDDRELVESARKGDREAFRTLFERYHRRAY
                     ALAFGVLRNQDDALDVVQDAFIKAHKYLDKFEGNSSFYTWLYRIVMNLAIDHLRKHRR
                     AKPVELDETHLEEGAAGDDTLLPKMLGGNPGRALMDKEIRARIDQALAELSENHRSVL
                     VMRELEGMSYEEMAQAMNCSKGTIMSRLFHARKNMQKRLADLFDKTVANDQEPGSSEE
                     DAAASGSRG"
     gene            1310..4225
                     /locus_tag="VFV99_17425"
     CDS             1310..4225
                     /locus_tag="VFV99_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061153.1"
                     /translation="MLYAFAALGALAIVMPVLGLVLGTSRATAMALLGIGGLAAMLVV
                     IGGLVMGFIAPRRRFGRDRDLARWVGTRHAPIASDLLSSVELANVPARPGAPSAELVD
                     ALIESTASELDTIEPETLLPTRDVPKARGYAIGAAVLNIALLVLVPAVMKTGWKRLFL
                     SPPSPYDGAQLSALPLVGDLRAQLAYPAYSKRKDSDLKSSSGDLRGLPGTKVRLSGRM
                     LVPAAAVELVIENGQVGSLDNAKHIAAKLSGDQMTAELTIDASARYRVAVVMPTGERK
                     IEQTPRAIEAEPDQAPAVQLMAPADVLDVANLRRVELAFVIEDDFGVTSAELVWEGGK
                     DRGRKAIPLDGSVPGRVQGKLMWDIADVQVPSGGNVRYWIEAKDNDSVTGPNIGKSRE
                     LSLRVVSPRERHEETLDRQQAVAEKLLHNLGGRLVGFGDNLTAREEYSHELRDAVLEL
                     SSINAAYEKDPHASDLMRKALTQMHDRLDRFAAAEQKLFPKGATKAPAGRFAAIDPRV
                     NAQLEDDLLVLADWLDRERMEGLLDINDEIAAHQKRLADLLAQYNRTKDPRLLDEIDR
                     EMRALDRSYAELEKHRKGMAEDVLDQYVHRDAVQAQQGTSCIAEVRALVAAGKTQEAQ
                     TKLEQCQQQQGKGASALEGSLANLRGDKFGEEQKKLDEVMNELADVAKDQDDIAAEAN
                     RIFESYARKADDIAKDHQREASKKVSALVDKLRKRLGAINEAGLTPFAKEELDIVERR
                     LNDVEHMVDDGDLAEALGMARQAKQSLDTIAGELEAAINDDPKSKWADATQDALDGIE
                     RTAPVAKELIDELSSLSPKPEQIMSADDLKALDRLRRRQAMNRQRTDKLGDRTKQMGA
                     DLPGDAAAELGKKLGAASEQMGKADDRMKGKDPSGSREAARAAADALAKARDRARNAA
                     RQAQEGATNEDEPIRIPGADEYKAPERFREDLQEGSKVPAPKGYEQMKDQYYKDISK"
     gene            4222..>5328
                     /locus_tag="VFV99_17430"
     CDS             4222..>5328
                     /locus_tag="VFV99_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061154.1"
                     /translation="MTRRALVTATVALLVLAGTAAARRDRTVTGDVIKAARFLTTSRV
                     EEAKGLLDDLKKRAPDAVEVKWLEAEYAFQTGDYAGAEKLLDKIPDDAVDGMVGQTKK
                     LAVSTRAVTETFVEKKSPKGHFIVRYASGSPDAAIAELAGEVLDTAWETVGDDLGLKP
                     ADPIRVEILGAPADLAKLSPLTESEIEKTGTIALSKYNKLMVVSPRATLFGYPWMDTL
                     AHEYTHIVVSRLAYDSVPVWLQEGIARFEQTRWRRPPEVVLNATEQALLTAGLKKGRL
                     ISFDEMHPSMAKLPSQDAAALAYAEVYALVGWMHEQIGYKGLRDALAAQKDGKSARRA
                     VSEALKKDWGAVDKEWRAHLKVSADTKARAGKPIK"
BASE COUNT          979 a         1727 c         1807 g          815 t
ORIGIN      
        1 cttcgtgcgc gtggtttgcc cgtcggcgcc gccacgcaac acgagcgcga gcgccgcgac
       61 agcacctgcg ctgactgcgc ccgtgatcac gtggcctcga tagtgatcga accaccgccc
      121 cacgcgcctc agccacccac cggccggcgc agcggcgaca tctgcagccg gcttgctcac
      181 agtttcgcgt tcccgatcga tgcccttgtc gatctcgcgc cacagcgcat cgagcttctt
      241 ctgcggcacc gcatcggcgg atagctcgag gtggccgcgc acgagctcac cgagctcgtc
      301 gaccgcttcg actttgcgac gcgacgcggg atcgcgcagc agctcttcga gctcgcgctc
      361 gtctgcctcg ccatcggcga gcgccatcag atcgatgtcg cgcggtttca tccgcgactc
      421 cccgacgctg cggcgtcttc ttcgctcgag ccaggttcct gatcgttggc gacggtcttg
      481 tcgaatagat cagcgagccg cttctgcatg ttctttcgtg cgtggaacag gcgcgacatg
      541 atcgtgccct tcgagcagtt catcgcctgc gccatttcct cgtacgacat gccttcgagc
      601 tcgcgcatga cgagcaccga ccggtggttc tcggacagct cggcgagcgc ctggtcgatc
      661 cgcgcgcgga tctccttgtc catcagcgcg cggccgggat taccgccaag catctttggc
      721 aacaacgtat cgtcccccgc ggcgccctcc tcgaggtgcg tctcgtccag ctcgacgggc
      781 ttggcccggc gatgcttgcg aaggtggtcg attgccaggt tcatgacgat ccgatacagc
      841 caggtgtaga agctggagtt gccttcgaac ttgtctaagt atttgtgagc cttgatgaaa
      901 gcgtcttgga cgacgtctaa ggcatcgtcc tgattgcgga gcacgccaaa tgcgagcgcg
      961 tacgcacgcc ggtggtagcg ctcgaatagc gtgcgaaatg cttcgcgatc gcctttgcgt
     1021 gcggattcaa ccagctcgcg gtcatcgact gggtcacgtc cggtggacgc tcgctggccc
     1081 tccttattca cgccgcggtc ggttatatca ccggaacccc cggtaataag atcgctgtca
     1141 ctcaggcgtt agacgaagcc ggagtgtcgg atcagccctc aggagctaaa ctgacggcta
     1201 ccgcgcggta gggcacacgg tggcagcgcc tcgcacttcg atttcaggga tctcagggta
     1261 cctggcccgc gttcgcgcgc tggtctaccg ccacgaggcg attcgggcgg tgctctatgc
     1321 attcgcggca cttggcgcgc tcgcgatcgt gatgccggtc ttgggcctcg tgctcgggac
     1381 gagccgggcc accgcgatgg ccttgctcgg catcggcggg ctcgccgcga tgctcgtcgt
     1441 gatcggcggg ctcgtgatgg gcttcatcgc gccgcggcgg cggttcggcc gcgaccgcga
     1501 cctcgcgcgc tgggtcggca ctcgccacgc cccgatcgcg agtgacctcc tctcgtccgt
     1561 cgagctcgcg aacgtgcccg cgcgtcccgg ggcgccgtcc gccgagctgg tcgacgcgct
     1621 gatcgaatcg accgcgagcg agctcgacac gatcgagccc gagacgctgc tgccgacgcg
     1681 cgacgttccg aaggcgcgcg gctatgcgat cggtgccgcg gtgctcaaca tcgcgctgct
     1741 cgtgctggtg ccggcagtga tgaagaccgg ctggaagcgg ctgttcctgt cgccgccatc
     1801 gccgtacgac ggcgcgcagc tctcggcgct accgctcgtc ggcgatctgc gcgcgcagct
     1861 cgcgtacccg gcatacagca agcgcaagga ttcggatctg aagtcgtcgt cgggtgactt
     1921 gcgcgggttg cccggtacga aggtcaggct gtcggggcgc atgctggtcc cagcggcggc
     1981 ggtcgagctc gtgatcgaga acggccaagt cggttcgctc gacaatgcaa agcacatcgc
     2041 cgcgaagctc tccggcgatc agatgaccgc ggagctgacg atcgatgcat cggcgcgcta
     2101 ccgcgtcgcg gtcgtgatgc cgacaggcga gcgcaagatc gaacagacgc cgcgcgcgat
     2161 cgaggccgag ccggatcaag cgcccgccgt gcagctgatg gcgcccgccg acgtgctcga
     2221 cgtcgcgaac ctccgccgcg tcgagctcgc gttcgtgatc gaagacgact tcggcgtgac
     2281 cagcgccgag ctcgtgtggg aaggcggcaa ggatcgcggc cgcaaggcga tcccgctcga
     2341 cggctcggtg cccggccggg tgcagggcaa gctgatgtgg gacatcgcgg acgtgcaggt
     2401 gcccagcggc ggcaacgttc gctactggat cgaagcgaag gacaacgaca gcgtcaccgg
     2461 cccgaacatc ggtaagtcgc gcgagctgtc gctgcgcgtc gtgagcccgc gcgagcgtca
     2521 tgaggagacg ctcgatcgcc agcaggcggt cgcggagaag ctgctgcaca acctcggcgg
     2581 tcggctcgtc ggcttcggcg acaacctgac cgcgcgcgag gagtactccc acgagctgcg
     2641 cgacgcggtg ctcgagctgt cgtcgatcaa cgcggcgtac gagaaggatc cgcacgcgtc
     2701 ggacctcatg cgcaaggcgc tgacccagat gcacgatcgg ttggatcggt tcgcggccgc
     2761 ggagcagaag ctgtttccga aaggcgcgac gaaggcaccc gccgggcgat tcgccgcgat
     2821 cgatccgcgc gtcaacgcgc agctcgaaga cgacttgctc gtcctcgccg actggctcga
     2881 tcgcgagcgg atggaaggcc tgctcgatat caacgacgag atcgccgcgc accagaagcg
     2941 gctcgccgat ctgctcgcgc agtacaaccg cacgaaggac cctcgcctgc tcgacgagat
     3001 cgatcgcgag atgcgcgcgc tcgaccgctc gtacgccgag ctcgagaagc atcgcaaggg
     3061 catggcagaa gacgtgctcg atcagtacgt gcatcgcgac gcggtgcagg cacagcaagg
     3121 cacgagctgc atcgccgagg tccgcgcgct cgtcgctgcg ggcaagacgc aagaggcgca
     3181 gacgaagctc gagcaatgcc agcagcagca gggcaagggc gcgagtgcgc tcgagggctc
     3241 gctcgcgaac ctgcgtggtg acaagttcgg cgaggagcaa aagaaactcg atgaagtgat
     3301 gaacgagctc gccgacgtcg caaaggatca ggacgacatc gcggcggaag cgaatcgcat
     3361 cttcgagtcg tatgcgcgca aggccgatga catcgcgaag gatcaccagc gcgaggcgag
     3421 caagaaggtc tccgcgctcg tcgacaagct gcgcaagcga ctcggcgcga tcaacgaagc
     3481 cggcctcacg ccgttcgcga aagaagagct cgatatcgtc gagcggcggc tcaacgacgt
     3541 cgagcacatg gtcgacgacg gcgaccttgc cgaagcgctc ggcatggcgc gccaggcaaa
     3601 gcagagccta gacacgatcg ccggcgagct cgaggccgcc atcaacgacg atccgaaatc
     3661 gaagtgggcc gacgcgacgc aggacgcact cgacggcatc gagcgtacgg cgccggtcgc
     3721 gaaggagctg atcgacgagc tgtcatcgct gtcgccgaag ccggagcaga tcatgagcgc
     3781 cgacgatctc aaggcgctcg atcggctgcg ccgccggcaa gcgatgaacc gccagcgcac
     3841 cgacaagctc ggcgaccgca cgaagcagat gggcgccgat cttccgggcg atgccgcggc
     3901 ggagctcggc aagaagctcg gtgcagcgtc cgagcagatg ggcaaagcag acgatcgaat
     3961 gaagggcaaa gatccgagcg gctcccgcga ggccgcgcgt gcggcagcag atgcactcgc
     4021 gaaggcgcgt gatcgcgcac gcaacgcagc tcgccaggcg caagaaggcg cgacgaacga
     4081 ggacgaaccg atccgcattc cgggtgccga cgagtacaag gcgcccgaac ggttccgcga
     4141 ggacctgcaa gaaggctcga aggtgccggc gccaaagggc tacgagcaga tgaaggacca
     4201 gtactacaag gacatcagca aatgacaagg cgcgcgctgg tcacagctac cgtcgccttg
     4261 ctcgtgctcg cgggaactgc ggcagcgcgg cgcgatcgca cggtcaccgg cgatgtgatc
     4321 aaggcggcgc ggttcctcac cacgtcgcgc gtcgaggaag cgaaggggct gctcgacgat
     4381 ctgaagaagc gcgcgcccga cgccgtcgag gtcaagtggc tcgaggccga gtatgcgttc
     4441 cagaccggcg attacgccgg cgcggagaag ctgctcgaca agattcccga tgacgccgtc
     4501 gacggtatgg tcggccagac caagaagctc gcggtctcga cgcgcgcggt gaccgagacg
     4561 ttcgtcgaga aaaagtcgcc gaagggtcac ttcatcgtgc gctacgcatc cggcagtccc
     4621 gatgccgcga tcgccgagct cgccggcgag gtgctcgata ctgcgtggga gaccgtcggc
     4681 gatgacctcg ggctcaaacc ggcagatccg atccgcgtcg agatcctcgg tgcgccggcg
     4741 gacctcgcga agctgtcacc gctcaccgag tccgagatcg agaagaccgg cacgatcgcg
     4801 ctgtcgaagt acaacaagct gatggtggtg tcgccgcgcg cgacgctgtt cggctatccg
     4861 tggatggaca cgctggcgca cgagtacacg cacatcgtcg tgtcgcgact cgcctatgac
     4921 tcggtgccgg tctggctgca agaaggcatc gcgcggttcg agcagacgcg ctggcggcga
     4981 ccgccggagg tcgtgctcaa cgcgaccgag caggcgctac tgaccgccgg gctcaagaag
     5041 ggtcggctga tcagcttcga cgagatgcat ccgtcgatgg cgaagttgcc gagccaggac
     5101 gcggcggcgc tggcatatgc cgaggtctac gcgctggtcg gctggatgca cgagcagatc
     5161 ggctacaagg gcctccgcga cgcgcttgcg gcccagaagg acggcaagag tgcccgccgt
     5221 gccgtctccg aggcactgaa gaaggactgg ggcgccgtcg acaaggagtg gcgtgcgcac
     5281 ctcaaggtat cggccgacac caaggcgcgc gccggcaaac cgatcaag
//
LOCUS       DASUFX010000434         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_69554,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000434 DASUFX010000000
VERSION     DASUFX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69554"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..786)
                     /locus_tag="VFV99_17435"
     CDS             complement(61..786)
                     /locus_tag="VFV99_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061155.1"
                     /translation="MLRWLAVGLALATCESAPQPTGVAGAAMPELRDHRAHGDAAAHL
                     QEAIALGRLADLREQALWIATTEMDSQASWQPYLVEIRAAAHSIARADDVASAGAGMG
                     LLGRACASCHDAMNVSPLVALVAAPTDERTLAAQSQRQQWAAARMWEGLLGSGDQRWL
                     DGANVLATTHLDIASTVHGKPNAETFALAERLREQAAQATMVVDPAERADVFGQIMET
                     CGSCHRILRPAPSNEKPRAVAGR"
     gene            complement(859..2085)
                     /locus_tag="VFV99_17440"
     CDS             complement(859..2085)
                     /locus_tag="VFV99_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5061156.1"
                     /translation="MFVRVAAAIGLAGLAACGNVTGHIGTTKVGDTSHAAERRSPVEE
                     LRTACVDGATTTYGSAALQRQPYLQQVTLTSAKLGWVSKNRDERVTITTPAGGIVGEL
                     SGVVEKAASRGDGELQIWSSATDLQPGTVYCYQLSDSTRTLNQRSGFRTAPSADSDQP
                     VRFLAFGDSGGGGADQYALADQMYTVPYDLIIHTGDLAYDDGTIGQYEDNVFGVYGDL
                     FRNIPFFPSAGNHDYKTMKGAPFREVFNLPGDNGEKWYSYDYGRVHFVALDTESDYTT
                     QMKWLEHDLAATKLPWKIIYMHRPPFSSGAHGSDTRLRNKLAPVLERHGVQLVLAGHD
                     HDYERMKPQNGVAYVVTGGGGVGTKSVGQSRFTAFSEDVIHFVYVEVGVDELTLHAID
                     ATGREFDSMTVPRVPS"
BASE COUNT          404 a          767 c          733 g          415 t
ORIGIN      
        1 cgcccgattc cgcagaagaa gcgcaagccg agcaagcagg acaagcgacg ctagcgcacg
       61 ctagcgcccg gcgaccgcgc gcggtttctc gttgctcggg gctggccgca ggatgcggtg
      121 acagctcccg caggtctcca tgatctgacc aaacacgtcg gcgcgctccg cgggatcgac
      181 gaccatcgtc gcctgcgccg cttgctcgcg cagccgctcc gccagcgcga acgtttccgc
      241 attcggcttt ccatgcaccg tgctggcgat gtcgaggtgt gtcgtcgcca gaacgttcgc
      301 gccatccagc cagcgttgat cgcctgagcc gagcaggcct tcccacatcc gggccgcggc
      361 ccattgctgg cgctggctct gcgcagcaag cgtgcgctcg tccgttggtg cggcgacgag
      421 cgcaaccagc ggcgaaacat tcatcgcgtc gtggcaggag gcgcatgcac gaccgaggag
      481 gcccatgccc gcgcccgcac tcgcgacatc gtccgctcgc gcgatgctat gtgcagccgc
      541 acggatctcg acgaggtagg gctgccagct cgcctgcgaa tccatctcgg ttgtcgcgat
      601 ccacagcgct tgctctcgga gatcagcaag gcgaccgagt gcgatggctt cttgtagatg
      661 cgcggcggca tcaccatgcg cacgatgatc gcggagctcc ggcatcgctg cgccggcaac
      721 accagtcggc tgcggcgcac tttcgcaggt cgccaacgcc aaaccgacag ccagccatcg
      781 cagcatgaca gacgtaggag catttgccgc gccagcggcg taagcgcgac gccgtcggcg
      841 aaatgcgcaa cgcaaggctt acgagggaac gcgcggcacg gtcatcgagt cgaactcgcg
      901 acccgtcgca tcgatcgcgt ggagcgtgag ctcgtcgacg ccgacctcga cgtagacgaa
      961 gtgaatcacg tcttcagaga atgcggtgaa gcgcgattgc ccgacactct tggtaccgac
     1021 cccaccaccg ccggtcacga cgtacgcgac accgttctgc ggcttcatgc gctcgtagtc
     1081 gtgatcgtgg ccggcgagca ccaattgcac gccgtggcgc tccaacacgg gtgcgagctt
     1141 gttgcgcagc ctcgtatcgg agccgtgcgc acccgacgaa aacggcggcc ggtgcatgta
     1201 gatgatcttc cacggcagct tcgtcgccgc gagatcgtgc tcgagccact tcatctgcgt
     1261 cgtgtagtcg gactcggtat cgagcgcgac gaagtgcacg cgaccgtagt cgtacgagta
     1321 ccacttctcg ccgttatcgc cgggaaggtt aaagacctcg cggaacggcg cgcctttcat
     1381 cgttttgtag tcgtgatttc ccgccgacgg aaagaacggg atgttgcgaa acagatcgcc
     1441 gtacacgccg aagacgttgt cctcgtactg accgatggtg ccgtcgtcgt acgcgagatc
     1501 gccggtgtgg atgatcaggt cgtacggcac cgtgtacatc tgatcggcga gcgcgtattg
     1561 atccgcgcca ccgccacccg aatcaccgaa cgcgaggaat cgcaccggtt gatcgctatc
     1621 ggcgctcggt gcggtgcgaa agccgctgcg ctggttcaac gtgcgcgtgc tgtcggagag
     1681 ctgatagcag tagaccgtgc ctggttggag gtcggtggcc gacgaccaga tctgcagctc
     1741 gccatctcct cgcgaggccg ccttctcgac gacgccggag agctcgccga cgatgccgcc
     1801 cgctggcgtc gtgatcgtga cgcgctcgtc gcgattcttc gatacccaac cgagcttcgc
     1861 gctggtcagc gtgacctgct gcagatacgg ctggcgttgc agagccgccg aaccgtatgt
     1921 cgtggtggcg ccatcgacgc aggctgtgcg cagctcctcg acaggagacc ggcgttcggc
     1981 agcgtgtgac gtgtcgccga ccttcgttgt cccgatgtga cctgttacgt tgccgcaagc
     2041 cgccaagccc gcgagcccga tggctgccgc tactcgtacg aacatcgaat ttccccccgc
     2101 aggtccggac aggtaagcac gtaaggtttc cctgtcgatg ctggaagaat tctgatcgcc
     2161 gatgctcgaa gatccctgat caggtcgcgt cacatcgtgc tccggggaca agtgtgctcg
     2221 cgcgcctcgc ccgcaccgcg gcatcggcgt tgcactgact ccgtacgtgg cacctgagcg
     2281 gcaccgactc gcgttgatcg atcggctact tcggccctt
//
LOCUS       DASUFX010000435         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_765554,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000435 DASUFX010000000
VERSION     DASUFX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765554"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFV99_17445"
     CDS             <1..806
                     /locus_tag="VFV99_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5061157.1"
                     /translation="LNTLIPKYATVNVLSDPDVRGGMKEYSDWPTFPQLFIRGEFVGG
                     ADIVRQMFDSGDLEKKLGDLVTPAKPVAMTVTPRAAQELGAALKEGSPGDVIHLTITQ
                     SWEHQLDLGAKEPTHVTIETNGITVQLDRASAQRANGVSIDFVEDTTGAGFKIENPNR
                     PPMVREIPAKDLKALIDSGKVKELYDVRTPKERELAMIAGSKLLDDATMKQIEQMPKD
                     TPLAFHCHHGPRSRAAAEHFVKQGFKTVYNLAGGIDAWSNDVDPSVKRY"
     gene            1499..2797
                     /locus_tag="VFV99_17450"
     CDS             1499..2797
                     /locus_tag="VFV99_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX5061158.1"
                     /translation="MRLSPVIFVALAACEGEGMLRHPMPDAPPIGPTANPARDIRETK
                     LQVDVTQLAGTATIAFAPGMAGASLEVGDLQISSVTRDGEDVPFAITGKQLDLGLPAN
                     DAQTMTVEIKYSYKLHENFDGLSMRGFTFTWPYFCGDLFPCHSAPSDGTLFSLDVQGV
                     PAGKTAIYAPTIANDAPSYMLAWSIDDYVETPLGTTTAGTELSVWHRASEATTAMNGT
                     QNLVAAFDWLEKTIGPYRFGTKAGTVSVAWPAGAYGGMEHHPRWHVDDNSLGSQETNV
                     HEAAHGWFGDGIRIQCWEDFVLSEGTVTYLAGRALDVVAPTVGAQTWTSYANELGGIP
                     PADPVWPQSCGTIDILNDNLYTNAPYIRGAFFYRAVADRVGAAKLDEALAAFYQAHAG
                     GSATMQDMLDTIKTVTGYDPTTCAQMWLRSTVRPTPAACP"
     gene            complement(2763..>3458)
                     /locus_tag="VFV99_17455"
     CDS             complement(2763..>3458)
                     /locus_tag="VFV99_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061159.1"
                     /translation="GGMISSVRDQLAFVEAELDADAGSKGTLRPAMRLSQESQLERQG
                     DNESLSWQIDSAGRYGQTGGTAGFRAFVGFDPKTRRGLVILAATRTSAIDTTATQIFR
                     MLANEDIKPPAFPDAAAVAAFAGTYNFRGLPLTLVAAGKRLYVQDPSAAAKYRMVPIT
                     DHEFWVEALRSIVVFEKDGATIKRAIFILGNGENQLSAPRCPDTGCPTQEPTAPPPAT
                     TPPTGKPPASGGR"
BASE COUNT          598 a         1157 c         1139 g          564 t
ORIGIN      
        1 tcctcaacac cttgatcccc aagtacgcca cggtcaatgt gctatcggac ccggatgtgc
       61 gcggcggcat gaaggagtac tccgactggc cgacgttccc ccagctgttc atccggggtg
      121 agttcgtcgg tggcgccgat atcgtgcgcc agatgttcga cagcggcgac ctcgaaaaga
      181 agctcggtga tctggtcacg ccggccaagc ccgtggcgat gaccgtgacc ccgcgcgccg
      241 cccaggagct gggcgcggcg ctcaaggaag gctcgcccgg cgatgtcatc cacctgacga
      301 tcacgcagag ctgggagcac cagctcgacc tcggtgccaa ggagccgacg cacgtgacga
      361 tcgagacgaa cggcatcacc gtgcagctcg accgcgccag cgcgcagcgc gcgaacggcg
      421 tgtcgatcga cttcgtcgag gacacgaccg gcgccggctt caagatcgag aacccgaacc
      481 gcccgccgat ggtgcgcgag attcccgcga aggatctcaa ggcgctgatc gacagcggca
      541 aggtcaaaga gctctacgac gtgcgcacgc cgaaggagcg cgagctcgcg atgatcgccg
      601 gcagcaagct gctcgacgac gcgacgatga agcagatcga gcagatgccg aaggacacgc
      661 cgctcgcgtt ccactgccac catggcccgc gcagccgcgc cgctgccgag cacttcgtga
      721 agcagggctt caagacggtc tacaacctcg cgggcggcat cgacgcgtgg tcgaacgacg
      781 tcgacccgtc cgtcaagcgc tactaacgca ctcgccgggg tcgtcgtggc ttgccgtgca
      841 cgagggctat gagctcgacg agctcgcgct gttcgtagac tttgtactcg atgacaaact
      901 gcgtgcgacc gagctgtaat cggtagagtc ctcgcgagcg ctgcccagca tacgggaatt
      961 gggcgagcgt cttacttgcc gcctcgagct cgtcgcgcag ctggcttggg ccttggccgc
     1021 gttcctccca ccgcgcgact tctcgatcga ccgcttggtc gacctcgtcc gtgacgctga
     1081 tgccaaagtt cttcggcgcg tcggagcttc tcgaggactt caccgatcgg cctcacgcgg
     1141 ccggcagcac tgtctgccaa gccccgctcg atcgagcgca gatcctcttc ggtgtactcg
     1201 aagtcgccgc ccggctgctc gatcgtcact tcgacctcgt cgccatcggg cagcatcaca
     1261 tttcgaatca cgacttcgcg attcttcacg gtcgccgtga atcgcatcaa catgcctcca
     1321 gagtagcaaa cacacggcgc gcaccacagc aacccacgcg ccagcgcaac cgcgcgatct
     1381 cgcgagctac gctgtccggt ttggccggac gccggacgcg gacacgcgga gaccggacaa
     1441 ctaccggacg catttcgctt gaacgtcgac ggcgctcgct gcatcgtcgc cgccgctcat
     1501 gcgtttgtcc cccgtgatct tcgtcgcgct ggccgcgtgc gaaggcgaag gcatgcttcg
     1561 ccacccgatg cccgacgcgc cgccgatcgg accgaccgcg aacccggcgc gcgacatccg
     1621 cgaaaccaag ctccaggtcg acgtgaccca gctcgccggc acggcgacga tcgcgttcgc
     1681 gccgggtatg gcgggggcat cgctcgaggt cggtgacctg cagatcagct cggtcacccg
     1741 cgatggtgaa gacgttccgt tcgccatcac cggcaagcag ctcgacctcg ggctgccggc
     1801 gaacgacgcg cagacgatga ccgtcgagat caagtacagc tacaagctgc acgagaactt
     1861 cgatggccta tcgatgcgcg ggttcacgtt cacgtggccg tacttctgcg gcgatttgtt
     1921 cccgtgtcac tctgcgccga gcgacggcac cttgttctca ctcgacgtgc aaggcgtgcc
     1981 ggcgggcaag accgcgatct atgcgccgac gatcgcgaac gacgcgccgt cgtacatgct
     2041 cgcgtggagc atcgacgatt acgtcgagac gccgctcggc acgacgaccg cgggcaccga
     2101 gctctcggtg tggcatcgcg cgagcgaagc gacgaccgcg atgaacggta cgcagaacct
     2161 ggtcgccgcg ttcgactggc tcgagaagac gatcggtccg tatcggttcg gcacgaaggc
     2221 cggcacggtg tcggtcgcgt ggcccgccgg cgcgtacggc ggcatggagc accatccgcg
     2281 ctggcatgtc gacgacaact cgctcggcag ccaggagacg aacgtgcacg aggccgcgca
     2341 cggctggttc ggcgacggca ttcgcatcca gtgctgggaa gacttcgtgc tgagcgaagg
     2401 cacggtcacg tacctcgcag gtcgcgcgct cgacgtggtg gcgccgaccg tcggtgcgca
     2461 gacgtggacg agctacgcga acgagctcgg cggaattccg cccgccgatc cggtgtggcc
     2521 gcagtcgtgc gggacgatcg acatcctgaa cgacaacctg tacacgaacg cgccgtacat
     2581 ccgcggcgcg ttcttttatc gcgctgtggc cgacagggtc ggcgcggcga agctcgacga
     2641 agcgctcgcc gcgttctatc aagcgcatgc cggcggcagc gcgacgatgc aggacatgct
     2701 cgacacgatc aagacggtga cgggctacga cccgacgacg tgcgcgcaga tgtggctgcg
     2761 ctctaccgtc cgcccgacgc cggcggcttg cccgtaggcg gagtggtcgc cggaggtggc
     2821 gccgtaggct cctgtgtcgg acagcccgtg tcggggcagc gcggcgccga gagctggttc
     2881 tcgccgttac caaggatgaa gatcgcgcgc ttgatcgtgg cgccatcctt ctcgaacacg
     2941 acgatgctgc gcagcgcctc gacccagaac tcgtgatcgg tgatcggaac catgcggtac
     3001 ttggccgcgg cactcgggtc ctgcacgtag agccgctttc cggcggccac gagcgtgagc
     3061 ggcagcccgc ggaagttgta cgtgccggcg aacgcggcga cggcggctgc gtcggggaac
     3121 gcgggcggct tgatgtcttc gttcgcgagc atgcggaaga tctgcgtcgc ggtggtgtcg
     3181 attgccgagg ttctcgtcgc tgcgaggatg acgaggccgc gacgcgtttt tggatcgaag
     3241 ccaacaaacg cgcggaaccc ggcggtgcca ccggtctgcc cgtaacgccc ggcgctgtcg
     3301 atctgccagc tgagactctc gttgtcgccc tgtcgctcga gctggctctc ctgtgagagc
     3361 cgcatcgccg ggcggagcgt gcctttactg ccggcgtcgg cgtcgagctc ggcttcgacg
     3421 aatgcgagct ggtcgcgcac gctcgagatc atgccgcc
//
LOCUS       DASUFX010000436        15763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_348948,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000436 DASUFX010000000
VERSION     DASUFX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348948"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..624
                     /locus_tag="VFV99_17460"
     CDS             88..624
                     /locus_tag="VFV99_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061160.1"
                     /translation="MTKALAFCVLLAACGKQAEPGADWSQKPLDATIENKVKNVPFKI
                     SVPKGWKFDAVGTRKEDDPDEITKEWRPDVKDYFSEPSVTVAYAAIPAKDLDSFLKYV
                     MVDDKKDVIAKKEQTPDGFVIVYHTKNKGIVRVELQKSKGDVALTCRASQAKTGGVPS
                     PDATMAWLEKMCGSLTLL"
     gene            662..1198
                     /locus_tag="VFV99_17465"
     CDS             662..1198
                     /locus_tag="VFV99_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061161.1"
                     /translation="MRTAVVLCVLVAACRKESEPRTDWSQTALDTTIESTVKDVPFTL
                     SLPRSWKFSASHERKEVDPEQLTKEWRPDLKDFYDEPSVTVAYAGAPAKTLDAFVKET
                     LLDPTKDVIEKEARTADGYVIAYHTPGKGVVRVEMLKTKGDVSLYCRGSQARGGGVPS
                     PLATLAWLEKVCDSLTLL"
     gene            complement(1252..3729)
                     /locus_tag="VFV99_17470"
     CDS             complement(1252..3729)
                     /locus_tag="VFV99_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061162.1"
                     /translation="MEGFLRVVCILLAAGCLHPRQVECGDGRVCPEGFECVHLDTYDQ
                     DLCALPEQLAACKDHNQFAECTLAGMTPARCYDGVCLPGGCGNGRQDPEEACDDGNNI
                     VGDGCAADCLSDETCGNGRLDPVRGEQCDDGDLIGHDGCASGCVPEPARWAPTSLLSP
                     FARRLHGMAFDVDRRTVVLFGGLGGTSTEPEVFADTWEYDERGWRQITPPVAPQARSG
                     AAMAYDAAHHRVVLFGGLALNDTWLWDGTSWTAVDTPVAPPVGRTTQWTMAYDSGRER
                     IVIVGADTMPKQTWEFDGTTWARLADAPTTSGFGQGNGLAYDPSRGRLVLVQDNGAAS
                     KTSEFDGTAWVEKAEQMPLLGKAALAYDPITKHVLAVGLANPNMPYTSLAQTWSWDGQ
                     SWSRIADPPTNFEAGVAVTDPFTPAVIEFGGMLSPSLGALDQTLTWNGSQWQLLPLRR
                     EPGVPMYATMASDPRRGRAVLYDSIGRLTWILDGYTWQRMTSQIAVRGLQAMAYDDAR
                     DELVMFGGSDPNGPDFDETWRWNGTWTQASPATHPSARIGAAMTYDPVAKRLVLFGGV
                     TQPRTNPTYVSETWTWDGTNWTNVTPATNLPPGRVAPAFAYDPVRQRAVLFGGSGSGA
                     PVGDTWEWDGTQWTQRMSVVGPAARIGASLTWDAARRRLVLFGGNNGATFAPIIYSDT
                     WEWDGTSWQQLPITGPVLAEHRAFTNGDSGVFITATDPQNSAGAVTYQLRRDSANTYE
                     ACRTQIDADGDGAVGCADLDCWSVCSPLCPPGGACDPAAPHCGDGACSSVESCTSCSA
                     DCGACPTICGDGACNGGETATSCPGDC"
     gene            complement(3766..3978)
                     /locus_tag="VFV99_17475"
     CDS             complement(3766..3978)
                     /locus_tag="VFV99_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061163.1"
                     /translation="MASSRIARFAAGEKVTVLVGSKAGEHASVEHVVWSDRDRCHYFY
                     LEQPGRKLSRGYLADELADGMGDTLG"
     gene            complement(4176..5165)
                     /locus_tag="VFV99_17480"
     CDS             complement(4176..5165)
                     /locus_tag="VFV99_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5061164.1"
                     /translation="MKTSVLVTGATGFLGEHLCRVLVEQGHIVRGLARTRSAVLESIG
                     VEHVRGDVLAGDELDRALDGVSAVFHLAGAVSRDPDDGQRMMRLHVDGTRRVLDRMAA
                     AGVRRMVLASTSGTIGVSKREEILDENAPYAEEIVAGWPYYASKIYQERLAFEHGERL
                     GIEVVAVNPSLLLGPGDRRLSSTGDVRKFIKRQIPSIPDGGINFVDARDAAIATANAL
                     GSGKSGQRYLLGGPNWTTKEFFARLGRIANVSPPRLKLPPKVNKWGASIVEELYRWRG
                     KEPPVDRISVEMAEHYWWIDSSKAQHELGFAPRDPQLTLVDTVSYLRQGVDPD"
     gene            complement(5162..9838)
                     /locus_tag="VFV99_17485"
     CDS             complement(5162..9838)
                     /locus_tag="VFV99_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5061165.1"
                     /translation="MGAGNGHNASKGGYFLETEAQWGAGSPTKPWPDALKTPLPPLRE
                     PITGKPRLDVGEIFRGKRILLIGTTGFVGKVALSMLLDRYPDVGAVYCLVRPGAGNTA
                     DERFYKKVATVEVFDPLRAKYGDGYEAFMRSKIIAVPGDIGRPLCNFTEEQIKETKVD
                     VIINSAGLVSFAPSLESALRINAMGAKNVLDLAKKLNARLVHVSTCFVAGQREGDVWE
                     DEPIVGYFPRSSAIARDWGNEDELLDRDFDPAAEIADCQKIIDQARDQANDRQHISVF
                     REKAAESLRAQRRDPDDEDDLKIAVARERKIWLNDVLTKLGMERARHWGWTNTYTYTK
                     SLGEQIILSDKSVPVTIVRPAVVESSVRYPFPGWNEGFNTTAPLMYLMLKGHRSVPVG
                     KDTALDVIPVDFIASGMLLATAAILSNQHEPVYQLGSSDANRISSKRLTQLTGLAVRR
                     HHRDKADRGEDKLRSKLRARLEAMPVSYEEFEARSAPMFKRIADGLIKVIDEKLPKWG
                     APRIEALAERARDELQKVSIFTGQINELVELFKPFTTDHDIAFRCDNMRALWARVSPA
                     DQDKLLWAPHLVDWRAYWLDTHFPGLQKWTFDKLDEEFGAKPKTVYTYKELIELFEST
                     IKLHRNRPALRLLRKEEGGEPISYTYGQMGDLAWQGAGVLRQQGVGPGSRVMLMSENR
                     PEWGISYFAILLAGAAAVPLDKELTLAEVQNLMKVSDAKAIVMSRKVAERLAGEANLA
                     IPVGPEDDEDDINIVWSPAHPALAQFFGRSVLAYDELLTEPDVSVGAVRPEVKGDTLA
                     SLIFTSGTTGTPKGVMLTHKNFTSVVSKLSSLFTLYKHDTLLSVLPLHHTFEFSAGFL
                     MPLLHGASIDYLEEIEADSLARALEDEGVTGMVGVPALWQLLERKIYKNVSDAGVLVE
                     KAFDSIVDLNRSLRDKLPWDVGTGKLLFFPVHRKLGGRMRLLISGGSALPPDTMKAFR
                     GLGFNLYEGYGMTESAPVLTVTRPGDKLIPGSVGRPLPGIDVRIDNPDANGIGEVIAK
                     GPNVMTGYFENPDATAQTIINGWLHTGDLGKIDEEGNLYIVGRKKEMILGPSGENVYP
                     DELEELYGDSDYVKEVSVVALPGDAGHETVAALIVPDYEAKPGDREYVREQVREHIKK
                     VSKGLPLYKRLKVFHLWDFDLPKTSTRKIKRREVVKELQRLERAAKGGADVKQLAQAS
                     PANSSSWILDVLADVSQKKRGTITSATPLADLGFDSLMFTELAVAIESAGVTLPDPAE
                     LTGLETVADVEKLVSRLGAKQELQRGVPGKKKDSKAAEADADDDINVPRPLVSLGRRA
                     LRGGMRALYERVLETDIYGSTHVPPFGGYIVAANHASHLDTGLVKYALGEQGEALVAL
                     GAKDYFFEDPVRRMYFENFTNVVPMERHGSLRESLRLAGEVIRDGYILLIFPEGTRSE
                     TGVMADFKPSLGYLAMQNKCGILPMYLHRTHDAMPKGRYLPKRGERVAAYVGPYLPYE
                     KIVELAGEKTRSAQYKNITHHVESVIRRLAPREAAWTLGDAGTTPMAEWLESQGKTVA
                     EEEAQP"
     gene            complement(9842..11452)
                     /locus_tag="VFV99_17490"
     CDS             complement(9842..11452)
                     /locus_tag="VFV99_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX5061166.1"
                     /translation="MRAIRPKLRSHAREHIVTIDTDSAPRIMFYGENLMMEDLPVGTR
                     VIYPKRPMTPVANPKAAIRYALNNPEDSEPLHALLRPGMKVTIAVDDISMPLPIMRTP
                     DVRQLVLEIVCEMLADHGVDDVHIVIALGLHRRMHDWEIKRMVGQKVWDEYWPDRLYN
                     HDGCDPDGMVLLGKTPHGELVEMNKRVAESDLTIYVNLNFVPMNGGHKSMAVGLCGYE
                     SLKAHHTPHGIVQSNSYMDPPKSYLSHSFKRQGDLVEKTLKVFHIETVLNNRAFDGPL
                     SFLTKNEDDFTETDRLKYQAMKWTLDKLPFTARREIFMRTPAAYELIACYAGACDPTH
                     NRTLQKQNEQNCVEVDGPADIMIYGIPYISPYNVNSKALNPLLVQVMALGYFYHMYRN
                     QPILRDGGVLIITHPCSDKFDPVHHPSYIEFFNRILTETTDAYTIEKKYQDELAYNPS
                     YIEMYRRGNAYHGAHPCFMWYWGQRGREKVSRVIVVGADNATVPQIMGWETAGSIAEA
                     IAMARGTMGRSAQISMMHHPPYMICDVM"
     gene            complement(11452..12234)
                     /locus_tag="VFV99_17495"
     CDS             complement(11452..12234)
                     /locus_tag="VFV99_17495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5061167.1"
                     /translation="MQRLPMHPGSPKYKNSAAFYDVDGTLIKINIVHAFAFYAARHAS
                     IAESTRRTIQTALSIPVFWAADKLSRKWFNEIFYRSYEGASEDRLVVLADELFEDVIK
                     PNIYPRAKELIDESRRAGVRQVLVSGALDFTMRPLAKYLGADDLIANQLEFVDHYATG
                     KLKKPFVAGATKADIMRAYAKQHDVDLAESWAYTDSFSDYPMLAVVGHPTACNPDFRL
                     RSLARSYDWPVLDLDEEPRRAADRRPQGLARPSGPRSIRRHN"
     gene            12444..12626
                     /locus_tag="VFV99_17500"
     CDS             12444..12626
                     /locus_tag="VFV99_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061168.1"
                     /translation="MSKRTHLFVGVIYLLLGAGLIATSFGFNPFGNNIGPTTETPSKD
                     KAPTKTGVPTDQLPKQ"
     gene            complement(12630..14390)
                     /locus_tag="VFV99_17505"
     CDS             complement(12630..14390)
                     /locus_tag="VFV99_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5061169.1"
                     /translation="MARDIRQLRAEASEATASGKYKRALAAYLELERLEPRDAQWSKR
                     VAETYRRLGKDKDAIGAYARSVDRYAQSGFLVQAIAVCKLILQIDPKNDDALQRLASM
                     NEQVGQGPTRAMSMVDANPKLAEDEKVQALRAGRGSGLHAAHTDGQRPRGDTLAPPIA
                     IARKVTPEALAIPRKFTPDQLAVPRKNTPVPVAVPSAQDESKRNRNPNSTPPPLSIPR
                     TKSKPISLPPDQPIESISLKDEVPDSFEREENSGVYVIPIDGEGAPEDATPIETAEEY
                     SEPLAVEIDAPDDDSDMSVDAIEDSTELEIDDIEDIEDIPLPEPRIVGIAAAEALAST
                     PLFAGLSQEALGSLVQQLTLVHLAKDEILFHEGDPGDALYVICEGEVSVSAEGPPRVE
                     MARMGAGAFIGEVALMTDQPRSATVTATLDAELLRIDRKTLSMVLATHGEVLSAVLRF
                     VRDRLVDRWQRTSPLFRPFDAGQRAEIAARFKFLEIEPGTKLLTPNKRPDGLYIVLAG
                     RFFVKRGGSNVATVGPGELIGETALLAGGVFQSEVIAAGKSLALCLPAGDFRDLILTH
                     PQVLEYIGEAAEHSRKLQIL"
     gene            complement(14421..15272)
                     /locus_tag="VFV99_17510"
     CDS             complement(14421..15272)
                     /locus_tag="VFV99_17510"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5061170.1"
                     /translation="MLAVSIDGMLVAPENATISIFDRGFLFGDGVFEVFRTWDEVAID
                     LDAHLTRLYASAAHLRLRTIDQDRAADAVQRTLASSGPGEKRIRVVLTRGPGALVARP
                     ATLGPGRAIVIVEPLPAQPKELSLAVVDWPLPRRTGVAHKTLAYLDHVLARELAANAG
                     ADEALRLGADGDVVECATANVFIVSGGAVATPPTEIGILPGVTRARVLAACAATGIKA
                     AERRLTLAEVQGADEMFVTSAVRGVVPVTRLDGNPRAAGPVTRRIIDGYLAGLRQQTV
                     APSGDPI"
     gene            complement(15297..>15763)
                     /locus_tag="VFV99_17515"
     CDS             complement(15297..>15763)
                     /locus_tag="VFV99_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061171.1"
                     /translation="SGAGDIQPPPGTPLSGVSTKIADAWTAAERALVRVHALRPRERL
                     VVWEKEDATGDAVSRYRAIAEAGGVAQLAAEYRIGALYHDLAISLLVEPLAADLRRTL
                     RPGAYGFLKKAITAYKASLAVGVPAHEMDSVWRLAAQSDMRAAQDVLGEAAQ"
BASE COUNT         2654 a         5208 c         5123 g         2778 t
ORIGIN      
        1 gtttcccacg cgtttggtac ccgcgggatg tcgtcgtcct ccgccacagc tggaatgtag
       61 cgccgcggct gcgctagggt gcggcccatg acgaaagccc tcgcgttctg tgtgctgctc
      121 gccgcgtgtg gaaagcaggc cgagcccggc gctgattgga gtcagaagcc gctcgacgcc
      181 acgatcgaga acaaggtcaa aaacgtaccg ttcaagattt cggtcccgaa gggctggaag
      241 ttcgacgcgg tcggtacgcg caaggaagac gatccggatg agatcacgaa ggagtggcgc
      301 ccggacgtga aggactactt cagcgagccg agcgttacgg tggcctacgc agcgattccg
      361 gcaaaagacc tggacagctt cttgaagtac gtgatggtcg acgacaagaa agacgtcatc
      421 gcgaaaaaag agcagacacc cgacggattc gtgatcgtct atcacacgaa gaacaaaggc
      481 atcgttcgcg tcgagctgca gaagagcaag ggcgacgtag cgctcacgtg ccgtgcgtcg
      541 caggcgaaga ccggcggcgt accgagcccc gacgccacga tggcatggct cgaaaagatg
      601 tgcggctcgc tcacgctgct ctgagctggt agcccgcacc gtggctgcta cggtgcacgg
      661 catgaggacc gcggtcgtgc tctgcgtgct cgtcgcagcg tgccgcaagg aatcggaacc
      721 ccgcaccgac tggagccaga ccgcgctcga cacgacgatc gagagcaccg tcaaagacgt
      781 gccgttcact ctgtcgctgc ctcgaagctg gaagttctcc gcatcccacg agcgcaagga
      841 agtcgatccg gaacagctca caaaagagtg gcgcccggac ctgaaggact tctacgacga
      901 accgagcgtg accgttgcct acgcaggcgc accggccaaa accctggacg cgttcgtaaa
      961 ggagaccctg ctcgatccga ccaaggacgt gatcgagaag gaagcacgga ccgccgacgg
     1021 ctacgtcatc gcgtatcaca caccgggcaa aggcgtcgtt cgcgtcgaga tgctcaagac
     1081 caagggcgat gtgtcgctct actgccgcgg gtcgcaggca cgcggcggcg gtgtgccgag
     1141 ccccctggcg acgctggcgt ggctcgagaa ggtctgcgac tcgctgacac tgctctaacc
     1201 ggaagatgtt ccggcaggtg atccgatgcg gcctctagtc aaacgcaagc gctaacagtc
     1261 accggggcac gaggtcgcgg tctcgccgcc gttgcatgcg ccgtcgccgc agatcgtcgg
     1321 acacgcaccg cagtccgcac tgcacgacgt gcaactctcg accgagctgc acgccccgtc
     1381 accgcagtgc ggagccgctg gatcgcacgc cccgcccggc ggacatagcg gcgagcacac
     1441 cgaccagcag tcgagatccg cacagccgac ggcaccgtca ccatcggcat cgatctgggt
     1501 tcgacacgcc tcgtacgtgt tcgccgaatc gcggcgcagc tggtaggtca ccgcgccggc
     1561 gctgttctgt ggatccgtcg cggtgatgaa cacgccgctg tcgccgttcg tgaatgcgcg
     1621 atgctcggcg agaaccgggc ccgtgatcgg aagctgctgc cagctcgtgc catcccattc
     1681 ccacgtgtcg ctgtagatga tcggtgcgaa cgtcgcaccg ttattgccgc cgaacagcac
     1741 gagcctccgg cgcgccgcat cccaggtcag gcttgcgcca atgcgagccg ctggcccgac
     1801 gacagacata cgttgcgtcc attgcgtgcc atcccattcc caggtatcgc cgaccggagc
     1861 gccactgccg ctgccgccga acagcaccgc gcgctgacgc actggatcgt acgcgaacgc
     1921 cggtgcgacg cgtccgggtg gaagattcgt cgccggcgtc acgttcgtcc agttcgtgcc
     1981 gtcccacgtc caggtctccg agacgtacgt cggattcgtt cgcggctgcg tgacgccacc
     2041 gaacaggaca agacgcttcg cgacgggatc gtaggtcatc gctgcgccaa tgcgcgccga
     2101 cggatgcgtt gccggcgacg cctgggtcca cgtcccgttc cagcgccatg tctcgtcgaa
     2161 gtcagggcca ttcggatcgc tgccaccgaa catcacgagc tcgtcgcgtg catcgtcgta
     2221 tgccatcgcc tgcagcccac gcactgcgat ctgcgatgtc atgcgctgcc acgtgtagcc
     2281 gtcgaggatc caggtcaacc ggccgatgct gtcgtagagc acagcgcgtc cacgacgcgg
     2341 atcgcttgcc atcgtcgcgt acatcgggac accaggctcg cgccggagcg gcagcaattg
     2401 ccactggctt ccgttccagg tgagcgtctg atcgagcgcg ccgagcgacg ggctcagcat
     2461 gccgccgaac tcgatcaccg ccggcgtgaa cggatcggtg acggcgacac cggcctcgaa
     2521 gtttgtgggt ggatccgcga tccgactcca gctctgaccg tcccagctcc acgtctgcgc
     2581 gagcgaggtg tacggcatgt tcgggttggc caggccgacg gcaagcacgt gcttggtgat
     2641 cggatcgtac gcaagcgctg ccttcccgag gagcggcatc tgctccgcct tctcgaccca
     2701 cgcggtgcca tcgaattcag atgtcttcga cgcggcgccg ttatcctgaa cgagcaccaa
     2761 gcgcccgcgc gacggatcgt acgcgagccc attgccctgc ccgaagcctg acgtcgtcgg
     2821 cgcatccgcg agccgcgccc aggtcgtgcc gtcgaattcc cacgtctgct ttggcatcgt
     2881 gtctgcgccg acgatgacga tgcgctcgcg accactgtcg tacgccatcg tccactgcgt
     2941 ggtgcggccg accggaggcg cgaccggggt atcgaccgcc gtccagctcg tgccgtccca
     3001 cagccaggtg tcgttgagcg cgaggccgcc gaacaacacg acacgatggt gcgcggcgtc
     3061 gtacgccatc gcggcgcccg agcgagcctg cggagcgaca ggcggtgtga tctggcgcca
     3121 gccgcgctcg tcgtactccc aggtgtcggc gaacacctcc ggctccgtcg aggtaccacc
     3181 gagcccgccg aacaacacga cggtgcgccg atcgacgtcg aaggccatcc catgcagccg
     3241 gcgcgcaaac ggcgacaaca acgaggttgg cgcccaccgc gctggctcgg gaacacaccc
     3301 actcgcgcag ccgtcgtggc cgatcaaatc gccatcatcg cactgctcgc cgcgaaccgg
     3361 atccagccga ccgttcccgc acgtctcgtc ggacaagcag tcggcggcgc aaccatcacc
     3421 gacgatgttg ttgccgtcgt cgcaggcttc ctcgggatcc tggcggccgt tgccgcaacc
     3481 gcccggcaag cacacgccgt cgtagcagcg cgcgggtgtc atgccggcga gcgtgcactc
     3541 ggcgaactga ttgtggtcct tgcacgcggc gagctgctcg ggcagcgcgc aaagatcctg
     3601 gtcgtaggtg tcgaggtgca cgcactcgaa gccctcggga cacacgcgcc catcgccgca
     3661 ttcaacttgc cgcggatgca agcaccccgc tgcgagcagg atgcacacga cccgcagaaa
     3721 accctccacc cgaccatctt acggcgaatc gaggtgggcg tgggatcagc cgagcgtgtc
     3781 gcccatgccg tcggcgagct cgtcggcgag gtagccgcgc gaaagcttgc ggcccggctg
     3841 ctcgagatag aagtagtggc agcgatcgcg gtcgctccac accacgtgct cgacagaggc
     3901 atgctcgccg gccttgctac cgaccagcac ggtgaccttc tcaccagccg cgaagcgggc
     3961 gatgcgtgag ctcgccatct tgcttcgagt tataccgcgg actggcttat cgaggtgctg
     4021 cgccgtcacg accccccggc tcataccacg cacgctctac aacgcttcaa cttcctagtc
     4081 gctcccggtg ttcaccggcg atggcacatc gagtcatcga cgctgcacct tcgcggcctc
     4141 acctaacgta cacgtcgcgc aaacgctgcg acgctctagt cgggatcgac gccctggcgc
     4201 agatagctga cggtgtcgac gagcgtcagc tggggatcgc gcggcgcaaa gccgagctcg
     4261 tgctgcgcct tcgacgaatc gatccaccag taatgctctg ccatttcgac gctgatgcga
     4321 tccaccggcg gctctttgcc gcgccagcgg tagagctcct cgacgatcga cgcgccccac
     4381 ttgttcacct tcggcggcaa cttgagccgt ggcggcgaga cgttcgcgat gcgaccgaga
     4441 cgcgcaaaga attccttcgt cgtccagttc gggccgccga gcaggtaacg ctgcccggat
     4501 ttgccgctgc cgagcgcgtt cgccgtcgcg atcgcagcat cgcgcgcgtc gacaaagttg
     4561 atgccgccat ccggaatcga tgggatctgg cgcttgatga acttgcggac gtcgcccgtc
     4621 gacgacaggc ggcgatcgcc ggggccgagc aagaggctcg ggttgacggc gacgacctcg
     4681 atgccgaggc gctcgccgtg ctcgaacgcg aggcgctcct ggtagatctt cgacgcgtag
     4741 tacggccaac cggcaacgat ctcttcggcg tatggcgcgt tctcgtcgag gatttcttcg
     4801 cgcttggaga cgccgatcgt gcccgaggtc gacgcgagca ccatgcggcg cacgcccgcg
     4861 gcggccatgc gatcgagcac gcggcgcgtg ccatcgacgt gcagacgcat catgcgctga
     4921 ccgtcgtccg gatcacgcga gactgcacct gcaaggtgga atacagcgga tacaccgtcc
     4981 agtgcgcgat cgagctcgtc gcccgcgaga acgtcgccgc ggacgtgctc gacaccgatg
     5041 ctctcgagca ccgcagagcg ggtacgcgcg aggccacgca cgatgtggcc ctgctcgacg
     5101 agcacccgac acaggtgctc acctaggaat ccggtcgcgc ccgtaacgag cacggaggtt
     5161 ttcatggctg ggcctcctct tcagcgacag tcttgccttg tgactcgagc cactcggcca
     5221 tcggcgtggt gcccgcgtcg ccgagcgtcc acgcagcttc gcgcggcgcg agccggcgga
     5281 tcacgctctc gacgtgatgg gtgatgttct tgtactgcgc ggatcgggtc ttctcgcccg
     5341 ccagctcgac gatcttctcg tacggcagat agggaccgac gtacgcggca acgcgctcgc
     5401 cgcgcttcgg taggtaacgg cccttcggca tcgcatcgtg cgtgcggtgc aagtacatcg
     5461 gcaggatgcc gcacttgttc tgcatcgcga ggtacccgag cgacggcttg aagtccgcca
     5521 tcacgccggt ctcgctgcgc gtgccctcgg ggaagatgag caggatgtag ccatcgcgga
     5581 tcacttcgcc ggcaagacgc agcgattcgc gcagtgagcc gtggcgctcc atcggcacca
     5641 cgttcgtgaa gttctcgaag tacatccggc gcaccggatc ctcgaagaag tagtccttcg
     5701 cgccgagcgc gacgagcgct tcgccttgct cgccgagcgc atacttgacc aggccggtgt
     5761 cgaggtggct cgcgtggttc gccgcgacga tgtacccgcc gaacggtggc acgtgcgtcg
     5821 agccgtagat atcggtctcg agcacgcgct cgtacagcgc gcgcataccg ccgcgcaacg
     5881 cgcgacgacc gagcgacacg agcggacgcg gcacattgat gtcgtcgtcg gcatcggcct
     5941 cggcggcctt gctgtccttc ttcttgccgg gaacgccgcg ctgcaattcc tgcttcgcgc
     6001 cgagccgcga cacgagcttc tcgacatccg cgaccgtctc gagcccggtg agctccgcgg
     6061 gatccggcag cgtgacgccg gccgattcga tcgcgaccgc gagctcggtg aacatcagcg
     6121 agtcaaaccc gaggtcggcg agcggcgtgg cgctcgtgat cgtgccgcgt ttcttctgcg
     6181 agacgtcggc gagcacgtcg aggatccacg acgacgaatt tgccggcgac gcctgcgcca
     6241 gctgcttcac gtctgccccg cccttcgcgg cgcgctcgag tcgctgcagc tctttgacga
     6301 cctcgcggcg cttgatcttg cgcgtggacg tttttggcag atcgaagtcc cacaggtgga
     6361 agaccttgag ccgcttgtag agcggcaggc ccttgctgac cttcttgatg tgctcgcgca
     6421 cttgctcgcg cacgtactcg cgatcgccgg gcttggcttc gtagtctggc acgatgagcg
     6481 ccgcgaccgt ctcgtgacct gcatcgcctg gtagagcgac gacgctgacc tctttgacgt
     6541 agtcgctgtc gccgtagagc tcctccagct cgtcgggata gacgttctcg ccgctcggcc
     6601 cgaggatcat ctccttcttg cggccgacga tgtagaggtt gccctcctcg tcgatcttgc
     6661 cgaggtcgcc cgtgtgcagc cagccgttga tgatcgtctg cgccgtcgcg tcgggattct
     6721 cgaagtagcc cgtcatcacg ttcgggccct tcgcgatcac ctcgccgatg ccgttcgcgt
     6781 cggggttgtc gatgcggacg tcgatgccgg gcagcggccg accgaccgag cccgggatca
     6841 gcttgtcgcc gggccgcgtc accgtgagca ccggcgccga ctcggtcatg ccgtagcctt
     6901 cgtacaggtt gaagccgagg ccgcggaacg ccttcatcgt atccggcggc agcgctgagc
     6961 cacccgagat gagcaggcgc atgcggccgc cgagcttgcg gtgcaccggg aagaacagca
     7021 gcttgcccgt gccgacgtcc cacggcagct tgtcgcgcag cgaccgattg agatcgacaa
     7081 tcgaatcgaa cgccttctcg acgaggacac cggcgtccga cacgttcttg tagatcttgc
     7141 gctcgagcag ctgccacagc gcgggcaccc cgaccatgcc ggtgacgcct tcgtcttcga
     7201 gggcgcgcgc gagcgagtcg gcctcgatct cctcgaggta atcgatcgac gcgccgtgaa
     7261 gcagcggcat caagaagcct gcggagaact cgaacgtgtg gtgcagcggc agcacgctga
     7321 gcagcgtgtc gtgcttgtac agcgtgaaca gcgacgacag cttgctcacc accgacgtga
     7381 agttcttgtg cgtgagcatc acgcccttcg gcgtgccggt cgtgccgctc gtgaagatca
     7441 acgacgcgag cgtgtcgccc ttcacctcgg ggcgcaccgc gccgaccgag acgtccggtt
     7501 cggtgagcag ctcgtcgtag gcgagcaccg agcggccgaa gaactgcgcg agcgccggat
     7561 gcgccggcga ccacacgatg ttgatgtcgt cctcgtcgtc ttcgggcccg accgggatcg
     7621 cgagattcgc ctcgccagcg agccgctcgg cgaccttgcg cgacatcacg atcgccttcg
     7681 cgtcgctcac cttcatgagg ttctggacct cggcgagcgt cagctccttg tcgagcggca
     7741 ccgcggcggc accggcgagc aggatcgcga aatacgagat gccccactcc gggcggttct
     7801 cgctcatcag catcacgcgg ctacccgggc caacgccctg ctggcgcagc acgcccgcac
     7861 cttgccaggc gaggtcgccc atctggccgt acgtgtacga gatcggctcc ccgccctctt
     7921 ccttgcgcaa gaggcgcagc gccgggcgat tgcgatggag cttgatcgtc gactcgaaca
     7981 gctcgatcag ctccttgtac gtgtagacgg tcttcggctt tgcgccgaat tcttcgtcga
     8041 gcttgtcgaa cgtccacttc tggaggccag ggaagtgggt gtcgagccag tacgcgcgcc
     8101 agtcgacgag atgcggcgcc cagagcagct tgtcttggtc cgccggcgac acacgagccc
     8161 acagcgcgcg catgttgtcg cagcggaacg cgatgtcgtg gtcggtcgtg aacggcttga
     8221 acagctcgac gagctcgttg atctgccccg tgaagatcga caccttctgc agctcgtcgc
     8281 gcgcgcgctc tgcgagcgcc tcgatgcgtg gcgcgcccca cttcggcagc ttctcgtcga
     8341 tgaccttgat caggccgtcg gcgatgcgtt tgaacatcgg cgccgaccgc gcctcgaact
     8401 cctcatagga aaccggcatc gcctcgagcc gcgcgcgcag cttgctgcgc agcttgtcct
     8461 cgccgcgatc ggctttgtcg cgatgatgcc tgcgcaccgc gaggccggtg agctgagtca
     8521 atcgcttgct cgagatccgg ttcgcgtcgg acgagccgag ctgatagacc ggctcgtgct
     8581 ggttcgacag gatcgctgca gtcgcgagca gcatgcccga agcgatgaag tcgaccggaa
     8641 tgacgtcgag cgcggtgtcc ttgcccaccg ggacactacg gtgccccttg agcatgaggt
     8701 acatcagcgg cgcggtcgtg ttgaagcctt cgttccaacc ggggaacgga tagcgtacgg
     8761 agctctcgac gaccgccggc cgcacgatcg tgacgggcac cgacttgtcg ctgaggatga
     8821 tctgctcgcc gagcgacttc gtatacgtgt acgtgttcgt ccagccccag tgccgcgcgc
     8881 gctccatgcc cagcttcgtg agcacgtcgt tcagccagat cttgcgctcg cgcgcgacgg
     8941 cgatcttcag atcgtcctcg tcgtcgggat cgcggcgttg cgcgcgtagc gactccgccg
     9001 ctttctcgcg gaacaccgag atgtgctggc gatcgtttgc ctgatcgcga gcctgatcga
     9061 tgatcttctg gcagtcggcg atttccgccg ccggatcgaa gtcacgatcg aggagctcgt
     9121 cttcattgcc ccaatcgcgt gcgatcgcgc tcgaccgcgg gaagtagccg acgatcggct
     9181 cgtcttccca cacgtcgcct tcgcgctggc cagcgacgaa gcaggtcgac acgtggacga
     9241 gtcgcgcgtt tagcttcttc gcgagatcga gaacgttctt tgcgcccatc gcgttgatgc
     9301 gcagcgcgct ctcgagcgac ggcgcgaacg acacgaggcc cgcgctgttg atgatgacgt
     9361 cgaccttggt ctctttgatc tgctcttccg tgaagttgca gagcggccgg ccgatgtcgc
     9421 cgggcaccgc gatgatcttc gagcgcatga acgcctcgta gccatcgccg tacttcgcgc
     9481 gcagcggatc gaacacctcg acggtcgcga ccttcttgta gaagcgctcg tcggcggtgt
     9541 taccggcgcc tgggcgcacg aggcaataga cggctccgac gtccggataa cgatcgagaa
     9601 gcatggacag cgcgaccttg ccgacgaagc cggtcgtgcc gatgagcaga atgcgcttgc
     9661 cgcggaaaat ctcgccgacg tcgagccgcg gcttaccggt aatcggctcg cgcagcggtg
     9721 gcagcggcgt cttcagcgca tccggccacg gcttggtagg cgagcctgcg ccccactgcg
     9781 cctcggtctc gaggaagtag ccacccttcg acgcgttatg tccgttgccc gcgcccatgg
     9841 ctcacatcac gtcgcagatc atgtacggcg ggtgatgcat catcgagatc tgcgcgctgc
     9901 gacccatcgt gccgcgcgcc atcgcgatgg cttcggcgat cgagccggcc gtctcccagc
     9961 ccatgatctg cggcaccgtc gcgttgtcgg caccgacgac gatcacgcgg ctcaccttct
    10021 cgcggccgcg ctggccccag taccacatga agcacggatg cgcgccgtgg tacgcgttgc
    10081 cgcgccggta catctcgatg tagctcgggt tgtatgcgag ctcgtcttgg tacttcttct
    10141 cgatcgtgta cgcgtccgtc gtctcggtga ggatgcggtt gaagaactcg atgtagctcg
    10201 ggtggtgcac cggatcgaac ttgtccgagc acgggtgcgt gatgatcaga acgccgccat
    10261 cgcgcaggat cggctggttc cgatacatgt gatagaagta gccgagcgcc atcacctgca
    10321 cgagcagcgg attgagcgcc ttagagttca cgttgtacgg cgagatgtac gggatgccgt
    10381 agatcatgat gtcggcaggt ccgtcgacct cgacacagtt ctgctcgttc tgtttctgca
    10441 gcgtgcggtt gtgcgtcgga tcgcacgcgc ccgcgtagca agcgatcaac tcgtacgccg
    10501 ccggcgtgcg catgaagatc tcgcggcgcg ccgtgaacgg cagcttgtcg agcgtccact
    10561 tcatcgcctg gtacttcagg cgatcggtct cggtgaaatc gtcctcgttc ttcgtcagga
    10621 acgacagcgg gccgtcgaac gcgcggttgt tcagcacggt ctcgatgtga aagaccttga
    10681 gcgtcttctc gacgaggtcg ccctgccgct tgaacgagtg gctcaagtag ctcttcggcg
    10741 gatccatgta cgagttggac tgcacgatgc cgtgcggcgt gtgatgcgcc ttgaggctct
    10801 cgtatccaca caggccaacg gccatcgact tgtggccgcc gttcatcggc acgaagttca
    10861 agttgacgta gatcgtgagg tcgctctcgg cgacgcgctt gttcatctcg acgagctcgc
    10921 cgtgcggcgt cttgccgagc agaaccatgc cgtcggggtc gcagccatcg tggttgtaca
    10981 ggcgatccgg ccagtactcg tcccacacct tctggccgac catccgcttg atctcccagt
    11041 cgtgcatgcg gcgatgcaga ccgagcgcga tcacgatgtg cacgtcatcg acgccgtgat
    11101 cggcgagcat ctcgcagacg atctcgagca cgagctgccg cacgtcgggc gtgcgcatga
    11161 tcggcagcgg catcgagatg tcgtcgaccg cgatcgtgac cttcatgccc gggcgcagaa
    11221 gcgcgtgcag cggctcgcta tcctccgggt tgttcagcgc gtagcgaatc gcagccttcg
    11281 gattcgcgac gggcgtcatc ggccgcttcg gatagatgac gcgcgtgccg accgggagat
    11341 cctccatcat gaggttctcg ccgtagaaca tgatgcgcgg cgccgagtcg gtgtcgatcg
    11401 tgacgatgtg ctcgcgtgcg tggctgcgga gcttgggacg gatggcgcgc attagttgtg
    11461 tctccgaatc gagcggggcc cactcggacg agcgagccct tgcgggcggc ggtcggctgc
    11521 acggcgtggc tcttcatcga gatcgagcac cggccagtcg tagctgcgcg cgagcgagcg
    11581 cagccggaag tcagggttgc acgcggtcgg atgtccgacg acggcgagca tcgggtagtc
    11641 gctgaacgag tccgtgtacg cccacgactc ggcgagatcg acgtcgtgct gcttcgcgta
    11701 cgcgcgcatg atgtcggcct tcgtcgcgcc ggcaacaaac ggcttcttca gcttgccggt
    11761 cgcgtagtga tcgacgaact cgagctggtt cgcgatcaag tcgtcggcgc cgagatactt
    11821 cgcgagcggc cgcatcgtga aatcgagcgc gccgctgacc agcacctgac gcacgccggc
    11881 gcgccggctc tcgtcgatca gctccttcgc gcgcggatag atgttcggct tgatgacgtc
    11941 ctcgaacagc tcgtcggcga gaacgacgag gcgatcctcg ctcgcgcctt cgtacgagcg
    12001 atagaagatc tcgttgaacc atttgcggct gagcttgtcg gccgcccaga agacgggaat
    12061 cgacagcgcg gtctggatcg tgcgacgcgt gctctcagcg atcgacgcgt gccgcgccgc
    12121 atagaacgca aacgcgtgaa cgatgttgat cttgatgaga gtcccgtcga cgtcataaaa
    12181 cgccgcggaa ttcttatact tcggcgagcc cgggtgcatc gggagtcgct gcactctggc
    12241 ccacggtgct gcgaagtgtc aatgcccaga aaccgtagcg aagtgcgaag catgctacgc
    12301 atccgctcgt gcgaatcccg gtctggctca cgctcggtat tgcggcgctc gttatcattt
    12361 tcggcgctta ccggatctac ctcgccactc ggcctgccac cgacgaggaa tcggacggac
    12421 caccgcgccg tggcttctac agaatgagta aacgtacgca cctgttcgtc ggggttattt
    12481 atctgctcct cggcgcaggc ctcatcgcga cctcgttcgg cttcaatccg ttcggcaaca
    12541 acatcggccc gaccaccgag acgccgtcga aggacaaagc gccgacgaag accggcgtac
    12601 cgaccgatca gctcccgaag cagtagtcgc tagaggatct ggagcttgcg actgtgctcg
    12661 gcggcttcgc cgatgtactc gagcacctgc gggtgcgtga ggatcaaatc gcggaagtcg
    12721 ccggccggaa gacagagcgc gaggctcttg cccgctgcaa tgacctcgct ctggaacacg
    12781 ccgccggcga gcagcgcggt ctcgccgatc agctcgcccg gaccgacggt cgcgacgttg
    12841 ctgccgccgc gcttcacgaa gaagcgacca gcgagcacga tgtagagacc atcggggcgc
    12901 ttgttcggcg tgagcagctt ggtgcccggc tcgatctcga ggaacttgaa gcgcgcggcg
    12961 atctcggcgc gctgaccggc gtcgaacgga cggaacagcg ggctcgtgcg ctgccaacga
    13021 tcgacgaggc gatcgcgcac gaagcgaagc actgcggaga ggacctcgcc gtgcgtcgcg
    13081 agcaccatcg agagcgtctt gcgatcgatg cgcagtagct cggcatcgag cgtcgcggtg
    13141 accgtcgcgg agcgcggctg atcggtcatc agcgcgacct caccgatgaa cgcgcccgcg
    13201 cccatgcgcg ccatctcgac gcgcggcgga ccttcagccg agaccgacac ttcgccttcg
    13261 cagatcacgt agagcgcgtc acccggatcg ccttcgtgga acaggatttc gtccttggcg
    13321 aggtgaacga gcgtgagctg ctggacgagc gaaccgagcg cctcttgcga gaggccggcg
    13381 aacaatggcg tcgatgcgag cgcttcggcg gccgcgatgc cgacgatgcg cggctcgggc
    13441 agcggaatgt cctcgatatc ctcgatgtcg tcgatttcga gctcggtcga gtcttcgatc
    13501 gcatcgaccg acatgtcgct gtcgtcgtcg ggtgcgtcga tctcgacggc caacggctcg
    13561 gagtattcct cggcggtctc gatgggcgtg gcgtcctcgg gtgcgccctc gccgtcgatc
    13621 ggaatgacgt agacgccgga gttttcttcg cgctcgaacg agtcgggcac ctcgtccttg
    13681 aggctgatcg actcgatcgg ttgatcgggc ggtagcgaga tcggcttgct cttcgtgcgc
    13741 ggaatcgaga gcggcggcgg ggtcgaattc ggattgcggt tgcgcttcga ttcgtcctgc
    13801 gcgctcggca ccgcgaccgg gacaggcgtg ttcttgcgtg gaaccgcgag ctgatcgggc
    13861 gtgaacttgc gcggaatcgc gagcgcttcc ggcgtcactt tgcgcgcaat cgcgatcggc
    13921 ggtgcgaggg tgtcgccgcg tggtcgctgg ccgtcggtgt gcgcggcatg aaggccgctg
    13981 ccgcgaccgg cgcgcagagc ctggaccttc tcgtcctcgg caagcttggg attcgcgtcg
    14041 accatcgaca tcgcgcgcgt cgggccttgg ccgacttgct cgttcatcga agcgagccgc
    14101 tgcagcgcat cgtcgttctt gggatcgatc tggaggatca gcttgcagac cgcgatcgcc
    14161 tggaccagga agccgctctg cgcatagcga tcgacggagc gggcgtaggc gccgatcgcg
    14221 tccttgtcct tgccgagccg ccggtaggtc tcggccaccc gcttggacca ctgggcatcg
    14281 cggggctcga ggcgctcgag ctcgagatag gccgcgagcg cgcgcttgta ctttccagag
    14341 gcagtcgcct cggacgcttc agcgcgcagc tgcctgatgt cgcgggccat cgaggttcgc
    14401 cactatatag cagtacgagg tcagatcgga tcgcccgaag gggcgacagt ttgttgccgc
    14461 aaacccgcga gatagccgtc gatgatccgc cttgtgacgg gcccggccgc acgcggattg
    14521 ccgtcgaggc gggtgaccgg caccaccccg cggaccgcgc tcgtcacgaa catttcatcg
    14581 gcgccctgca cctccgccaa agtcagccgc cgctcggctg ccttgatgcc cgttgccgca
    14641 caggccgcga gcacgcgtgc gcgggtcacc cccggcaaaa ttccgatctc ggtcggcggc
    14701 gtcgcgaccg caccaccgct gacgataaag acgttcgcag tcgcgcactc gacaacatcg
    14761 ccatcggcac cgagccgcag cgcttcatcg gcaccggcgt tcgccgcgag ctcacgggcg
    14821 agcacgtgat cgaggtatgc gagcgtcttg tgcgcgacac ccgttctgcg cggcagcggc
    14881 caatcgacga ccgccaggga cagctctttg ggttgcgccg gaagcggctc gacaatcacg
    14941 atcgcgcggc cgggaccgag cgtcgcgggg cgcgcaacca gtgcgccggg gccgcgggtc
    15001 agcacgacgc ggatccgctt ctcgccgggg cccgagcttg cgagcgtgcg ttgaacggcg
    15061 tcggcggcgc gatcctgatc gatggttctg agccggaggt gcgcagccga tgcgtatagg
    15121 cgcgtcagat gcgcgtcgag atcgatcgcg acctcgtccc acgtgcgaaa gacttcgaag
    15181 acaccgtcgc cgaacaggaa cccacgatcg aagatcgaga tcgtggcgtt ctctggagcg
    15241 accagcatcc cgtcgatcga gaccgcgagc acgcggcgag gatagcagcg aggccgctac
    15301 tgcgcggctt cgccgagcac gtcttgcgca gcgcgcatgt cggactgcgc ggcgagtcgc
    15361 cacaccgaat ccatttcgtg cgctggaacg ccgaccgcga ggctcgcctt gtacgccgtg
    15421 atcgccttct tcaagaagcc gtaggcgccc gggcgcagcg tgcgccgtag atcggcggcg
    15481 agcggctcga cgagcagcga gatcgcgagg tcgtgataca gcgcgccgat ccggtactcc
    15541 gcggcgagct gagcgacgcc acccgcttcg gcgatcgcgc ggtagcgact cacggcgtct
    15601 ccggtcgcgt cctctttctc ccacaccacg aggcgctcgc gcggacgcag cgcatggacg
    15661 cggacgagtg cgcgctcggc ggccgtccat gcatcagcga tcttcgtgga cacgccagat
    15721 agcggcgtgc ctggcggcgg ctggatgtca ccggcgccgc tgc
//
LOCUS       DASUFX010000437         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_488764,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000437 DASUFX010000000
VERSION     DASUFX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488764"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFV99_17520"
     CDS             complement(<1..424)
                     /locus_tag="VFV99_17520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061172.1"
                     /translation="MAVSQTAEYVALYRALETTETRREPLFRDPFARHLLTGSRLRAL
                     RLATMPGLRSMLERYADHRAPGARTSAIGRTRFIDDVVRAERARGIRYYVILGAGYDS
                     RAHRLVELKEARVFEVDRAETQAEKRRRIESVPGVRKDV"
     gene            complement(446..1186)
                     /locus_tag="VFV99_17525"
     CDS             complement(446..1186)
                     /locus_tag="VFV99_17525"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5061173.1"
                     /translation="MTDEVTIDALTGGWTIAQRKKGHRHSTDDLLTGWYAALVVPDAR
                     HILDLGSGIGSVGLIALWRSPHATLVAIEAQEISFALLEGNIMRNGLGDRVRAIHGDL
                     RDVRLPERFDLVTGSPPYWDVSEGIVPADSQKAHARFELRGDIRDYARAAHDALAENG
                     RFVFGFPTVQQHRARVAAQAAGLAVERLRHVIPRHGADPLFSLFACRRDADPGTDLVV
                     EPAHIVRDEHGDPTAMHRAVRAALGFSG"
     gene            complement(1191..>2072)
                     /locus_tag="VFV99_17530"
     CDS             complement(1191..>2072)
                     /locus_tag="VFV99_17530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5061174.1"
                     /translation="RRVAACLHAAATGTSHARPAGMLKAIAIVIVLLVPSLALSQAPP
                     AKTPAPTAKKAPATKAPTAKPSIVLVHGAWADGSSWDKVSPLLMAKGYNVVAVHLPMT
                     SPADDVAAVNRAIDRMPGDVVLVAHSYGGFVISEAGNNKKVKKLVFVDAFGLDDGESL
                     NALTKGNHQPFEKTLQVDSGGYAWMPIETIMSDFVPDMPADEQKLVFVKQAPISVKAF
                     DEVMKNPAWKTKKSWYVRGTQDRIIPPDAQAMMAKRMKATTVNVDAGHVSMLAKPNEV
                     AAVILQAAGAPTATASK"
BASE COUNT          334 a          687 c          688 g          363 t
ORIGIN      
        1 gtacgtcttt gcgcacgccg ggtacgctct cgatgcgccg ccgcttctcg gcttgggtct
       61 cggcgcgatc gacctcgaag acgcgcgctt ctttgagctc gaccaggcga tgcgcacgcg
      121 agtcgtaacc agcgccaagg atcacgtagt agcgaatgcc gcgagcacgt tcggcgcgca
      181 cgacgtcgtc gatgaatcgc gtgcgaccga tcgccgaggt gcgtgcaccg ggcgcgcggt
      241 ggtcggcgta gcgctcgagc atgctgcgta agcccggcat ggtggcgagg cgcagcgcgc
      301 ggagtcggct gccggtgagc agatggcgtg cgaacggatc gcgaaacagc ggctcgcggc
      361 gcgtctcggt cgtctcgagc gcacggtaca gcgctacgta ttcggctgtt tggctgactg
      421 ccacgcgggg tgagcgtaac cccgtctagc cgctgaatcc cagtgctgcg cgcaccgccc
      481 gatgcatcgc ggtcggatct ccgtgctcgt cacgaacgat gtgtgccggc tcgacgacca
      541 ggtcggtgcc agggtcggcg tcgcgacggc atgcgaacaa cgagaacagc ggatcggcac
      601 catgccgcgg gatgacatgg cggagccgtt ccacggcgag gcccgccgcc tgagcggcca
      661 cgcgcgcgcg gtgctgctgc acggtcggga aaccgaacac gaaccgcccg ttctcggcga
      721 gcgcatcgtg cgccgcgcgt gcgtaatcgc gaatgtcgcc gcgcagctcg aagcgcgcgt
      781 gcgctttctg cgagtcggcc ggcacgatgc cttcgctcac gtcccagtac ggggggctgc
      841 cggtgacgag atcgaagcgc tccggcaggc gcacgtcgcg gaggtcgccg tgaattgcgc
      901 ggacgcggtc gccgagaccg ttgcgcatga tgttgccttc gagcagcgcg aagctgatct
      961 cctgcgcctc gatcgccacg agcgtcgcgt gcggcgatcg ccacagcgcg atgagcccga
     1021 ccgagccgat gccgctgccg agatcgagga tgtgccgcgc atcgggcacg acaagggccg
     1081 cgtaccagcc cgtcagcaag tcgtcggtcg agtgacggtg gcccttcttg cgttgcgcga
     1141 tcgtccagcc gcctgtcaac gcgtcgatcg tcacctcgtc ggtcacacca ctatttactc
     1201 gcggtcgcgg tcggtgcgcc agcggcctgg aggatcacgg cggccacctc gttcggcttc
     1261 gccagcatcg acacgtgacc ggcgtcgaca ttgacggtcg tcgccttcat ccgcttcgcc
     1321 atcatcgcct gggcgtccgg gggaataatg cgatcctgag taccgcgcac gtaccacgac
     1381 ttcttcgtct tccacgccgg gttcttcatc acttcgtcga acgccttcac cgagatggga
     1441 gcttgcttga cgaacacgag cttctgctcg tcggccggca tgtctggcac gaagtcgctc
     1501 atgatcgtct cgatcggcat ccatgcgtat ccaccgctat cgacctgcag cgtcttctcg
     1561 aacggctgat ggttgccttt cgtcagggca ttcagcgatt cgccgtcatc gagaccgaac
     1621 gcgtcgacga agacgagctt cttgaccttc ttgttgttgc cggcctcgct gatcacgaac
     1681 ccgccgtacg aatgcgcgac aagcacgacg tcacccggca tgcgatcgat cgcccggttc
     1741 accgccgcga catcgtcggc cggcgaggtc atgggtaggt gcaccgcgac gacgttgtac
     1801 cccttcgcca tcagaagcgg cgagaccttg tcccacgacg agccatcagc ccatgcgcca
     1861 tggacgagca cgatcgaggg cttcgcagtc ggcgctttcg tcgcaggtgc cttcttcgcc
     1921 gttggcgcgg gagtcttcgc gggcggcgct tgcgagagtg cgagtgacgg aacgagcaga
     1981 acgatgacga tcgcgatggc cttgagcatg cccgcgggcc gagcatgcga tgtgccagtg
     2041 gccgccgcgt gcaaacacgc agcaacccga cg
//
LOCUS       DASUFX010000438         7077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1739348, whole genome shotgun sequence.
ACCESSION   DASUFX010000438 DASUFX010000000
VERSION     DASUFX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739348"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFV99_17535"
     CDS             <1..847
                     /locus_tag="VFV99_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061175.1"
                     /translation="HRLYQLAAGERWDPLVPMAPPEAPDEHVVLDDDEHDIERLSFFL
                     KGALDRLLERLAARGRALTALHVELTLRHAVGDTELRADCIKPAAPTLDGRALLRLVH
                     LRLTGMPPAAPVNAARLWADDVAATREQLAMFASKPRRDLRAANEALARVRAELGDDA
                     VVRAVLRDGHLPEASFGWERLAQVQQAAPVPKLVRPLVRRLYAKPQLLPPQARQVRDD
                     GWLLSGLEHGAVVRILGPYIVSGGWWQHELHREYHFAELRRGDCLWVFYDRNRRRWFC
                     QGSVE"
     gene            complement(848..1180)
                     /locus_tag="VFV99_17540"
     CDS             complement(848..1180)
                     /locus_tag="VFV99_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061176.1"
                     /translation="MLSACGDSIDTSIEDQITIEQGVYGLLISGCDTSGCKDQPAANE
                     HVVVYAAGSTGVFSQVTSDKHGVYQMNLVGGDYTLCTSSCTTVTVPPNEKVRYDWTSG
                     PGGGYWSE"
     gene            1333..2196
                     /locus_tag="VFV99_17545"
     CDS             1333..2196
                     /locus_tag="VFV99_17545"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5061177.1"
                     /translation="MPDDKPALPDDKRALPDDKPSFGTAEPNEPITRADFERAVRFLN
                     LSDLEIRDTLLRLAAQVVALTDELVRRIDKVEPEPAAPNTPAREVTNTVECAVVDAVP
                     VTLAKIRAAEVRDTGRVWIEIDQENKYDVTSPDIPCAELLHLCQARCCRMGFPLSTAD
                     LDEGVIRWDYGQPYMIRQRASDGFCVHNDPDTHGCTVHAHRPLVCRKYDCREDVRVWI
                     DYEKRIPAPNGVGDREQKDFDLMERVRKRHEAMIVENAALVQVFPDKEPHTGPALPQD
                     LRLFHKRPPGA"
     gene            complement(2193..3416)
                     /locus_tag="VFV99_17550"
     CDS             complement(2193..3416)
                     /locus_tag="VFV99_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5061178.1"
                     /translation="MARSSRSIIHVHHRGDRGVDGILRLIELASHDGPIEAMLSAMCD
                     EIAAIMEIEIASIYVVEDDKLVMRGNHGFPDSAIGNVALKVGEGITGLVAECMRPVSA
                     AHAAAEAAYKEVPGLGEEHFPVFAGVPLISGGAVVGVLVLQRRKKPFAADEVTLATAL
                     GAPVNLAVERRRATAVRSARLNGTAHAGDSVVLGRVGIVPTATAIGTAPFDGIADIEL
                     ELERVLGRLREDLARALRKLRDADDPLIGGVLDRFTLALCDQRLRERLVAAATDPTGL
                     RGVAKDYARAARRLGTPDERAVEIEELCAMIGMTLDARSSARPGTIWLADRVGAVAAL
                     IAVARGAAALVASDSASPAAIAIARAAKLPFITGVSGLFGWARTGDLLAVDGGTGTVL
                     VHPAPTEIERLRSAR"
     gene            3577..4002
                     /locus_tag="VFV99_17555"
     CDS             3577..4002
                     /locus_tag="VFV99_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061179.1"
                     /translation="MLRDDYIIRLIKQLGAFLARIAGKRKQEDFEGALAEAGKAWDDV
                     IGQPPRELVDVLDTHTLAEMLKEPVRMRAAAQILIEEGRAYAGKGDPAHAAVCYRRAW
                     ELFLEARVIEPSEDDLPVLTELARLVPANQLDPRYRVDV"
     gene            complement(3984..4895)
                     /locus_tag="VFV99_17560"
     CDS             complement(3984..4895)
                     /locus_tag="VFV99_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061180.1"
                     /translation="MTRAWIVDALGEAGFEQLAATIPGSELQSLLLEVMRRRASARSP
                     HDVLAQYRRDRFVRPAAIDQRTLVELDGHLLAAADGFDAIELSPVTPLGTSSTMGPTD
                     QHKVLSALRGTEVVSDPTNVLALECAGRLRAAPDTPVHYATCQRVIRAQALPKDKPGF
                     AAHFKIFCLASAGLETKDHAFSVATLTRHITTVHRALDRLEQHGYQFGARSVDILATP
                     ERAAVADRIAAAVNANRKLLEHPYYNGGIRYMFWVTHASGDRVPLIDGGLFDWVARLT
                     SNRRNVYVTTGAGAQLMAFAFRQTSTR"
     gene            complement(4905..5687)
                     /locus_tag="VFV99_17565"
     CDS             complement(4905..5687)
                     /locus_tag="VFV99_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061181.1"
                     /translation="MVDGTGIQGQTYTVQVLLPRHIELYAVGLLLALRRWRGDIVLAS
                     CSPEQVTVTVPTGDLPLYVQLFHTHPEDYASELIEAITWSPAWEERWEDTARRCPASI
                     VIAMTAQRPINYASMLLSFLAVLDTWLHTLDIDDRERVVLHWMPAKQLLTYHQYQFLR
                     TELGPCGPAVNVRVANATGRPGELLADTVGLAELGLPDLQILFTNRDPVAVVRRLRRY
                     VSSVFVGDRLEVPWVEEASIVPPERDALTLWDDSTPTPTPVW"
     gene            5782..>7077
                     /locus_tag="VFV99_17570"
     CDS             5782..>7077
                     /locus_tag="VFV99_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5061182.1"
                     /translation="MDEYAPLWCKSNFSFLEGASHAEELVEEAHRLGMRSLAITDRDG
                     VYGMVRAHVKAKELGIHLVCGAQMTVAPPSARLIASPVTLPRVGLHHDEHVGHGPGWG
                     AETDDLPPAVPTTGRRGRRKHSGPRAGMLPSVTRGRAKPRVPQLALGAAATANDAAPA
                     EPTSSQLVLLAIDRAGWANLVRLSTAGRRRCDKGESLVSWQEVCERAGGLIALWGGDA
                     SMLAGELEPSPQIIGDMRAAFGDRLYAILARHRRADDVPREARLRARAAAHHIPLVAA
                     TEVLYHSRARRPLQDVLTCIRHGVTLATAGRLIRGNDEHDLRPPHAFHKLYADESAAI
                     ARTLDIAARCTFSLGELRYRYPSERLPDGTTSAAYLRKLAYEGAGWRYGGDIPGNVRR
                     QLDAELAIIEELDYPGYFLTMYEIVSYCRRRDIMCQGRGS"
BASE COUNT         1196 a         2392 c         2362 g         1127 t
ORIGIN      
        1 gcatcggctc tatcagctcg cagccggcga acgctgggat ccactggtgc cgatggcgcc
       61 gcccgaggcg cccgacgagc acgtcgtgct cgacgacgac gagcacgaca tcgagcggct
      121 cagcttcttc ttgaagggcg cgctcgatcg gttgctcgag cggctcgcgg cgcgcgggcg
      181 tgcgctcacc gcgttgcacg tcgagctcac attgcgccat gccgtcggcg ataccgagct
      241 gcgcgccgat tgcatcaagc ccgcggcgcc gacgctcgac ggtcgcgctc tcttgcgcct
      301 cgtccacctg cggctcaccg gcatgccgcc ggccgcaccg gtcaacgccg cgcgtctgtg
      361 ggccgacgac gtcgcagcga cgcgcgagca gcttgcgatg ttcgcgagta aaccgcgccg
      421 cgatctgcgc gcggcgaacg aagcgctcgc acgcgtgcgc gccgagctcg gcgacgatgc
      481 agtcgtacgc gccgtgctgc gcgatggtca tttgccggaa gcgagctttg gttgggagcg
      541 actcgcgcag gtgcagcaag cggcgccggt gcccaagctc gtgcgtccgc tcgtgcgtcg
      601 gctctacgcg aagccgcagc tattgccacc gcaagcgcgt caggtgcgcg acgacggctg
      661 gctgctctcg ggcctcgagc acggtgcggt cgtccgcatc ctcgggccgt acatcgtgtc
      721 gggcggctgg tggcagcacg agctgcaccg cgaatatcac ttcgccgagc tgcgccgcgg
      781 cgattgcctg tgggtgttct acgatcgcaa tcgccggcgc tggttctgcc agggttccgt
      841 cgagtgatta ctcagaccag tagccgccac cggggccgct ggtccagtcg tagcgaacct
      901 tctcgttcgg cgggaccgtg accgtcgtgc agctagacgt gcacagcgtg tagtcgccac
      961 cgacgaggtt catctgatag acgccgtgct tgtcgctcgt cacttgcgag aagacgcccg
     1021 tcgagccggc cgcgtagacg acgacgtgct cgttcgccgc aggttgatct ttgcagcccg
     1081 acgtatcgca gcccgaaatc agaagcccgt acacgccctg ctcgatcgtg atctggtcct
     1141 cgatgctcgt gtcgatgctg tcaccacacg cagagagcac cgcaaccata agcaaatacc
     1201 gcaacatcgc ggctagtgat cagcaatgcg cgtgccgggc taaccccgcg gcatcactca
     1261 ccagcgctgt gaaaatggct gcgatcccag acatcgttgt catgatcgcg gcaacgcgag
     1321 attctgcgcg cggtgcccga cgacaagccc gcgctacccg acgacaaacg agcgctaccc
     1381 gacgacaaac cgagtttcgg cacggcggag ccgaacgagc ccatcacgcg cgcggatttc
     1441 gaacgcgctg tgaggtttct caacctgagc gatctcgaga tacgcgacac gctgctgcgg
     1501 ctcgcggcgc aggtcgtcgc gcttaccgac gagctcgtac gacgcatcga taaggtcgag
     1561 cccgagccgg ccgctccgaa cacgccggca cgcgaggtga cgaataccgt cgagtgcgca
     1621 gtcgtcgatg cggtaccggt gacgctcgcg aagattcgcg cagccgaggt gcgcgacacc
     1681 ggccgcgtgt ggatcgagat cgatcaagag aacaagtacg acgtgacgag ccccgacatc
     1741 ccgtgcgcgg agcttctgca cctgtgtcag gcgcgttgct gccggatggg ctttccgcta
     1801 tcgacggcgg atctcgacga aggtgtgatt cgctgggact acggtcagcc gtacatgatc
     1861 cgccagcgcg cgagcgacgg cttctgcgta cacaacgatc ccgatacgca cgggtgcacc
     1921 gtgcacgcgc accggccgct cgtgtgccgc aagtacgact gccgcgaaga tgtccgcgtg
     1981 tggatcgatt acgagaagcg catcccggcg ccgaacggcg tcggcgatcg cgagcagaag
     2041 gacttcgatc tcatggagcg cgtgcgcaag cgccacgaag cgatgatcgt ggagaacgcc
     2101 gcgctcgtgc aggtctttcc cgacaaggag ccgcataccg gcccggcgct gccgcaggac
     2161 ctacggctgt tccacaagcg accgccgggt gcttagcgtg cgctgcgcag ccgctcgatc
     2221 tcggtcggcg cgggatgcac gagcacggtg ccagtgccgc cgtcgacggc gagcaggtcg
     2281 ccggtgcgtg cccagccgaa caggcccgac acgccggtga tgaacggaag cttcgcggca
     2341 cgtgcgatcg cgatggccgc aggtgatgca ctgtcgctcg cgacgagggc tgcagcacca
     2401 cgcgcgacgg cgatgagcgc agcgactgca ccgacgcggt cggcgagcca gatcgtaccc
     2461 ggacgcgccg agctgcgtgc gtcgagcgtc atgccgatca tcgcgcacag ctcttcgatc
     2521 tcgaccgcac gttcgtcggg cgtgccgagg cgccgtgccg cgcgcgcata atccttcgcg
     2581 acgccgcgca ggcctgtcgg atcggtcgcc gccgcgacga ggcgttcgcg cagtcgctga
     2641 tcgcagagcg cgagtgtgaa gcgatcgagg acgccaccga tcagcggatc gtctgcgtcg
     2701 cgcagcttgc ggagcgcgcg ggcaaggtcc tcgcgcaggc ggccgagcac gcgctcgagc
     2761 tcgagctcga tgtccgcgat gccgtcgaat ggcgcggtac cgatcgcggt agccgtcggc
     2821 acgatgccaa cccggccgag cacgacactg tcaccggcgt gcgctgtacc gttcaggcgc
     2881 gccgagcgga ccgcagtcgc acgacggcgc tcgaccgcga ggttgaccgg ggcaccgagt
     2941 gccgtcgcga gcgtgacctc gtcggcagca aacggcttct tgcgccgctg cagcacgagc
     3001 acaccgacga cggcgccgcc gctgatcagc ggcacaccgg cgaacaccgg gaaatgctcc
     3061 tcgccgaggc cgggaacttc tttgtaggcg gcttcggccg cggcgtgcgc tgctgacact
     3121 ggccgcatgc actcggcgac gaggccggtg atgccttcgc cgaccttgag cgcgacgttg
     3181 ccgatcgcgg agtctggaaa gccatgattt ccgcgcatga cgagcttgtc gtcctcgacg
     3241 acgtagatgc tcgcgatctc gatctccatg atcgcagcga tctcgtcgca catcgcgctc
     3301 aacatcgctt cgatgggacc atcgtggctc gcaagctcga tgagtcgcag gatcccatcg
     3361 acaccgcgat caccgcggtg atgtacgtgg atgatcgaac gcgagctgcg agccacgaag
     3421 gcagcctgac atgaacgatg tttccggcgc gtttccgatc cgtcaacttc tgttgcggct
     3481 tcgcgggctt ccgctgcgat aacgatcagt acggatgggc agggcctggc cgcacggtcg
     3541 ggtaccgggc cgccgtccta tgcttacgaa gcaaccatgc tgcgcgacga ctacatcatc
     3601 cggctcatca agcagctcgg cgcgttcctc gcgcgcatcg caggtaagcg caagcaagag
     3661 gacttcgagg gtgcgctcgc cgaggcgggc aaggcgtggg acgacgtgat cggtcagccg
     3721 ccgcgcgagc tcgtcgacgt gctcgatacc cacacgctcg ccgagatgct gaaggagccg
     3781 gtgcggatgc gagcggccgc gcagattctg atcgaagaag gtcgcgccta tgcgggcaag
     3841 ggcgatccgg ctcacgccgc ggtctgctat cgccgcgcgt gggagctctt cctcgaagca
     3901 cgagtgatcg agccgagcga agacgacctg ccggtgctca ccgagcttgc tcggctcgtg
     3961 cccgcgaacc agctcgaccc gcgctatcgc gtcgacgtct gacgaaacgc gaacgccatc
     4021 agctgcgcgc ccgcgccggt cgtgacgtag acgttgcgac gattcgaagt gagccgcgcg
     4081 acccagtcga acaaaccgcc gtcgatcagc gggacgcggt cgccgctcgc gtgcgtcacc
     4141 caaaacatgt agcgaatgcc gccgttgtag tacgggtgct cgagcaactt gcggttggcg
     4201 ttgaccgcgg ccgcgatgcg atcggcgacg gcggcgcgct cgggtgtggc aagaatgtcg
     4261 acggagcgtg cgccgaactg ataaccgtgt tgctcgaggc gatcgagtgc gcggtgcacc
     4321 gtcgtgatgt ggcgcgtgag cgtcgcgacg gagaacgcgt ggtctttcgt ttcgaggccc
     4381 gcgctcgcaa gacagaagat cttgaagtgc gctgcgaagc ccggcttgtc cttcggaagc
     4441 gcttgcgcgc ggatcacgcg ctggcatgtc gcatagtgca ccggtgtatc aggcgcggcg
     4501 cgcaaccgcc cggcgcattc gagcgcgagc acgttcgtcg gatccgacac gacttcggtc
     4561 ccgcgcagtg ccgacagcac cttgtgctga tcggtcggtc ccatcgtcga cgacgtacca
     4621 agcggggtca ccggagagag ctcgatcgcg tcgaagccat ccgcggcggc gagcaggtgg
     4681 ccgtcgagct cgacgagcgt gcgctgatcg atcgcggcgg gacgcacgaa ccgatcgcgc
     4741 cggtactgtg cgagcacgtc gtgtggcgat cgcgcgctcg cgcgacggcg catcacttcg
     4801 agcaggagcg actgcagctc gctacctgga atcgtcgcgg caagctgctc gaagccagcc
     4861 tcgccgaggg cgtcgacgat ccatgcgcgc gtcacgcgcc gacgttacca aaccggcgtc
     4921 ggtgtcggcg tcgagtcgtc ccagagtgtc agcgcatcgc gctccggtgg cacgatcgaa
     4981 gcctcctcga cccacggcac ctcgaggcga tcgccgacga acacgctgct cacgtatcgg
     5041 cgcagccgcc gcacgactgc aaccggatcg cgattcgtga acaagatctg cagatcgggg
     5101 agcccgagct cggcgagccc gacggtatcg gcgagcagct cgcccggccg accggttgcg
     5161 ttcgcgacgc gcacgttcac cgcgggacca cacgggccga gctcggtgcg caggaattga
     5221 tactggtgat aggtgagcag ctgctttgcc ggcatccagt gcaagacgac gcgctcgcga
     5281 tcgtcgatgt cgagcgtgtg cagccacgta tcgagcacgg cgaggaacga cagcagcatg
     5341 ctcgcgtagt tgatcggtcg ctgcgccgtc atcgcgatca cgatcgaggc cgggcagcgg
     5401 cgcgcggtgt cttcccagcg ctcctcccat gccgggctcc acgtgatcgc ctcgatgagc
     5461 tcgctcgcgt agtcctccgg gtgcgtgtga aacagctgca cgtagagcgg caggtcgccg
     5521 gtcggcacgg tgaccgtcac ctgttcgggg ctacagctcg ccagcacgat gtcgccgcgc
     5581 caccggcgca gggcgagcag caggcccact gcatacagtt caatgtggcg aggcagcagt
     5641 acctggaccg tgtacgtctg accctgaatc ccagtcccgt cgaccatggg cgaggctgat
     5701 acaatggtcc gaccatcaat tcaacatgat gtttcgattg gcttgacggg catcacggat
     5761 aactgaacac tcgtacagtt aatggacgag tacgcgccgc tctggtgcaa gtcgaacttc
     5821 tcgtttctcg aaggcgccag tcacgccgaa gagctcgtcg aagaagcgca tcggctcggc
     5881 atgcgctcgc tcgcgatcac cgatcgcgat ggcgtctacg gcatggtccg cgcgcacgtc
     5941 aaagcgaagg agctcggcat ccatctcgtg tgcggcgcgc agatgacggt cgcaccgccg
     6001 agcgcgcgcc tgatcgcttc gccagtcacg ttgccgcgcg tcggtctgca tcacgacgag
     6061 catgtcggtc acggtccagg ctggggcgcc gagaccgatg acctgccgcc cgcggtgccg
     6121 acgaccggca ggcgagggcg ccggaagcat tccggcccaa gggccggaat gcttccttcg
     6181 gtgacacggg ggcgcgcgaa gccgcgtgtg ccgcagctgg cgctcggtgc cgctgcgacc
     6241 gcgaacgatg ctgcgccggc cgagccgacg tcgtcgcagc tcgtgctgct cgcgatcgat
     6301 cgcgcgggct gggcaaacct cgtgcgcctg tcgactgcgg gccgccgccg ctgcgacaag
     6361 ggcgagtcgc tcgtcagctg gcaggaagtc tgcgagcgcg caggtggcct gatcgcactc
     6421 tggggtggcg acgcgagcat gctcgcagga gagctcgagc cgtcgccgca gatcatcggc
     6481 gacatgcgcg ccgcattcgg cgatcgcctc tacgcgatcc tcgcgcgcca tcgccgcgcc
     6541 gacgacgtgc cacgcgaagc tcgcctgcgc gctcgcgcag ccgcacatca catcccgctc
     6601 gtcgccgcga ccgaagtcct ctatcactcg cgcgcacgcc gcccactgca agacgtgctc
     6661 acgtgtattc gccacggtgt cacgctcgcg accgcaggcc gcttgatccg cggcaacgac
     6721 gagcacgacc tccgtccgcc gcatgcgttc cacaagctct acgccgatga gtccgccgcg
     6781 atcgcgcgca cgctcgacat cgccgcgcgc tgcacgttct cgctcggcga gctccgctat
     6841 cgctatccgt cggagcgcct gcccgacggc accacctccg ccgcttacct ccgcaagctc
     6901 gcctatgaag gcgcgggatg gcggtacggc ggcgacatcc ccggcaacgt gcgccggcaa
     6961 ctcgatgccg agctcgcgat catcgaggag ctcgattacc ccggctactt cctcacgatg
     7021 tacgagatcg tctcgtactg ccggcggcgc gacatcatgt gccagggccg cggctcc
//
LOCUS       DASUFX010000439         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1044229, whole genome shotgun sequence.
ACCESSION   DASUFX010000439 DASUFX010000000
VERSION     DASUFX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044229"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFV99_17575"
     CDS             complement(<1..872)
                     /locus_tag="VFV99_17575"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5061183.1"
                     /translation="MKRLTPSDSMFLYGESREQMMHVGAMMPFGPLPNGNPDTLRDLM
                     NEIRGAAVHRPWNMRLRTPDFLWNPLQAWVEQDDVDIEYHVRRSALPSPGDERELGIL
                     VSRLHGHHVDFHRPPWEAHLIEGLDRGRFAMYIKVHHSLVDGFSAMRILAHALSTDPN
                     ETDRPMFFSIPPPAREPRPEGEGGVHFPELLAAVREQYGAAKTAVRALRKLLESARSG
                     DHDFVTPRAAPKSLLNARISKSRRFATQLIATARLKAVAKASGGTLNDVVLALCSASL
                     RRYLVEQNALPDAPL"
     gene            complement(892..1524)
                     /locus_tag="VFV99_17580"
     CDS             complement(892..1524)
                     /locus_tag="VFV99_17580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061184.1"
                     /translation="MEGDRERWNAKWRERAGELETEAPFLREHAIELPTRGKALDVAG
                     GAGRNAIWLARRGLDVTVVDVSDVALDKAEHRAKNAGLANRMKFVRHDLDADLPFAPL
                     FELVLVFHYLNRARRDDYVSLLHDGGTLIAVQPTVQNLERHASPSRQYLVELGEMEAW
                     ARELGMGIVVAREGWTDEGRHEAAVIAKRSSNVVEHSAHEDSGPQQGPYR"
     gene            1548..2171
                     /locus_tag="VFV99_17585"
     CDS             1548..2171
                     /locus_tag="VFV99_17585"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5061185.1"
                     /translation="MRSEASLKGSSARLWKLVEERAQRGADVGAIDQRIWDLFGEEWA
                     VMFTDLAGFSRQVAKFGIIHFLQIILEQKRLLLPVLEAHDGILIKIEADSLLVLFKKP
                     AQALACAIEMQQKCQAASARTAPEEQVLLCVGLGFGRILKIGDEDVFGHEVNIASKLG
                     EDVAKRHEVLVTRAFHDAVETPAGATWHEQRVDAAGEDVCWRLEYRR"
     gene            2180..>2501
                     /locus_tag="VFV99_17590"
     CDS             2180..>2501
                     /locus_tag="VFV99_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061186.1"
                     /translation="MDPIGAGLMLCVAGYIAYRAFRPRDKATAMLRSIARLHGGRLDK
                     HGTLQLIVDGFAIEVRALLPSDVNNKLRGETYWRIKLPPPGTTTFAIYPEGFKSHASK
                     ALGTR"
BASE COUNT          429 a          785 c          857 g          430 t
ORIGIN      
        1 agtggcgcat cgggcagcgc gttctgctcg acgagataac ggcggaggct cgccgagcac
       61 agcgcgagca cgacatcgtt gagcgtgcca cccgacgcct tcgcgactgc tttgaggcgt
      121 gccgtcgcga tcagctgagt cgcgaaacgg cggctcttgc tgatgcgcgc gttgagcaga
      181 gacttcggcg ctgcgcgcgg cgtgacgaag tcgtgatcgc cggagcgtgc actctcgagc
      241 agcttgcgga gcgcgcgaac cgcagtcttc gcagcgccgt actgctcgcg cacggcggca
      301 agcagctccg ggaagtgaac gccgccttcg ccttcgggac gcggctcgcg ggccggcggt
      361 gggatcgaga agaacatcgg ccgatcggtc tcgttcggat cggtcgacag cgcgtgcgcg
      421 aggatacgca tcgccgagaa gccgtcgacg agcgagtgat ggaccttgat gtacatcgcg
      481 aagcggccgc gatcgaggcc ctcgatcagg tgggcttccc acggcgggcg gtgaaaatcg
      541 acgtgatggc cgtgcagtcg cgacacgagg atgccgagct cccgctcgtc gccaggcgat
      601 ggcagcgcgg agcgccgcac gtggtactcg atatcgacat cgtcttgctc gacccacgcc
      661 tgcagcgggt tccacaagaa gtccggcgtg cgaaggcgca tgttccacgg gcgatgtacc
      721 gcggcgccgc ggatctcgtt catcaggtcg cgcagcgtgt cagggtttcc gttcggcagc
      781 ggcccgaacg gcatcatcgc cccgacgtgc atcatctgct ctcgcgactc gccgtagagg
      841 aacatcgagt cgctcggcgt gaggcgcttc acgttgtcga tcctagcagc ttcatcggta
      901 cggaccctgt tgtgggccgg aatcttcgtg cgctgaatgc tcgacgacgt ttgaagagcg
      961 tttggcgatc accgccgctt cgtgacggcc ttcgtcggtc cagccttcac gcgcgacgac
     1021 gatgcccatg ccgagctcgc gagcccaggc ctccatctca ccgagctcga cgaggtactg
     1081 gcggctcggc gacgcgtgcc gctcgaggtt ctgcaccgtc ggttgcaccg cgatcagcgt
     1141 gccaccgtcg tgcagcagac tgacgtaatc atcgcggcgg gcgcggttca ggtagtgaaa
     1201 cacgagcacg agctcgaaca gcggcgcgaa cggcaggtcg gcgtcgaggt cgtgacgcac
     1261 gaacttcatg cgattcgcga ggccggcatt cttggcgcgg tgctccgcct tgtcgagtgc
     1321 gacatcggag acgtcgacga cggtgacgtc gaggccgcgg cgggcgagcc agatcgcgtt
     1381 gcgtcctgcc ccgccggcaa catcgagtgc tttgccgcgg gtcggcagct cgatcgcgtg
     1441 ctcgcggagg aacggggcct cggtctcgag ctcgcctgcg cgttcgcgcc acttcgcgtt
     1501 ccagcgttcg cgatcacctt ccacccatct attgtaggct ggagggtatg aggagcgaag
     1561 cgtccttgaa gggaagctcg gcgcggctat ggaagctcgt cgaggagcgc gcgcaacgcg
     1621 gcgctgacgt cggtgcgatc gatcaacgaa tctgggacct attcggcgag gaatgggcgg
     1681 tgatgttcac cgacctcgcc gggttctcgc ggcaggtcgc gaagttcggc atcattcact
     1741 tcctccagat catcctcgag cagaagcggc tcctgcttcc ggtgctcgaa gcgcacgacg
     1801 gcatcttgat caagatcgaa gccgacagct tgctcgtcct gttcaagaag ccggcgcaag
     1861 cgctcgcgtg cgcgatcgag atgcagcaga agtgtcaggc agccagcgcg cgcaccgcgc
     1921 ccgaagaaca ggtgctgctg tgtgtggggc tcggctttgg ccggatcttg aagatcggcg
     1981 acgaggacgt gttcggtcac gaggtcaaca tcgccagcaa gctcggtgag gacgtagcga
     2041 agcgtcacga ggtcctggtg acccgcgcat tccatgatgc cgtggaaaca cccgcgggcg
     2101 cgacgtggca cgagcagcgc gtcgatgctg ccggcgaaga cgtttgctgg cggctcgagt
     2161 accggcgcta ggagctcgga tggatccaat cggcgcgggt ctcatgctct gcgtggcggg
     2221 ctacatcgcc tatcgcgcgt tccggccgcg cgacaaagcc actgccatgt tgcggtcgat
     2281 cgcgcgtctg cacggcggca gactcgacaa gcacgggacg ctgcagctca tcgtcgacgg
     2341 cttcgcgatc gaagtccgcg cgctgctgcc ttccgacgtg aacaacaagc tgcgcggtga
     2401 aacgtactgg cgcatcaagc tgccaccgcc ggggaccacg acgttcgcga tctaccccga
     2461 aggcttcaag agtcatgcga gcaaagcgct gggcacgcgc g
//
LOCUS       DASUFX010000440         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1876979, whole genome shotgun sequence.
ACCESSION   DASUFX010000440 DASUFX010000000
VERSION     DASUFX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876979"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFV99_17595"
     CDS             complement(<1..1241)
                     /locus_tag="VFV99_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5061187.1"
                     /translation="MTRAALLLLLIAACSSGSRRVGNQPSWRSNASKQGPVTFAPTSE
                     PAIRYNEPLRAPAPTPLGDAVLAAVKEAATASKVPVPAPDVRLFRACAELARIMPEDG
                     LLDYSVVEFALQRQGIIEPSPSLLVVWGAVDDTESIVEQLKAKLPESLKEGTMARVGI
                     GTAKRTDDGMGAIVFALQASGVATAPIPRSVAAGGSFTVDAVVDLRYRDTELFVTHDD
                     GKTERIPIDVNKQGAFKASVSCAKHNGKQQVEIAASDAAGSTVLANFPVWCGMDPPLM
                     ITIEPTHDDNVVVSAEEAEKRLLALMNRDRMAAGLPALLWDDRVAAVSRAHSEDMRKT
                     KIVAHISPTTGSAADRTRAAGIKTAVVLENVARAYGVGEAHSGLMNSPGHRANVLSSA
                     ATHVGIGVVLGEEVSGRREMF"
     gene            complement(1256..2956)
                     /locus_tag="VFV99_17600"
     CDS             complement(1256..2956)
                     /locus_tag="VFV99_17600"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX5061188.1"
                     /translation="MKRIALIAIVLAGCVDHGSGPQGKRIEASYIRENLLTAVPQDIQ
                     PMQVDLGGKVTYLGNKVVYVDGKDPKTPLAPGAAIRVMHYWRVNMPPGEQWRVFAYAR
                     GAQGSADFMNFGPTDMEIGYPTKKWKAGQIIQDVQDIVLRPDWRSPTVTLYVGLVEDG
                     KHQIGDRMPATGGNVIDRAIVARTIDVDLSKAPPPQGTVYIPHATGPITIDGMATEPG
                     WSSAATSPELITADGSADPVGKATARMTWDEQNLYLFVSIVDSDVYSQYKNHDEPLWK
                     ADCVEVFIDADGNRTGYVELQVNPNNATFDSFFATTRAQPGDEKWDANMETVVKVRGT
                     ADKAGDTDQGWDVEIAIPLAAVKGRHDTMAVRLPPEVGDRWRLNVVRVDYRSNGGSPG
                     VASWNRISYSDFHALDKMLTVVFADPSGSIVPKPPGADTGSGGSAGSAAPQTGSAGSA
                     APLSPIPQTGSATAPMLAPIAPAMIQGNAQGSAVSQGSGAPTTAPTAPAPTAPASKAP
                     ASKAPASKAPASKVPAPTAPPAPANAPHAGPTMAPTTVPRTATPPPTLPAATSNPKSP
                     "
     gene            complement(2953..4188)
                     /locus_tag="VFV99_17605"
     CDS             complement(2953..4188)
                     /locus_tag="VFV99_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5061189.1"
                     /translation="MTVTADDLVRATQKLRLAPTDWGAWLEVAAVLGALGATDEVELA
                     FATIGEGARVTGRVALAVACGRHLAELGSVRGPELIEQVIDTYATGGPHFAFTTPPSP
                     EDPPAVDWPPGEPLAQARAVIEELGAWLMKRQPTQVPPAPLLSALSKPGARALVGVMT
                     AKAYPAMTPIIDIGAPATQLYWIAHGTVTVSRPKFQPASAQSGPIAQLGELHSGAFFG
                     EIALVGGTKRTANVIAAEDVWLLEIPARALETAAQKHPQLAEVIAFHARARLLSNVLR
                     TSELFKPLDENVREELLGKFSSELIPQGMTFIRDGEANDALWVIVSGTARVSSGGAPV
                     AELQPGSAVGEMSLVSGAAAGADVTALEPLVLLKLAKKDFDAVVKRYPTVLAIVEKLA
                     KTRQQANQQMFQDASDLIV"
BASE COUNT          650 a         1511 c         1388 g          730 t
ORIGIN      
        1 aagaacatct cgcgccggcc cgatacttct tcgccgagca cgacgccgat gccgacgtgc
       61 gttgctgccg acgacaacac gttcgcacga tggccggggc tgttcatcag acccgagtgc
      121 gcttcgccga cgccgtacgc gcgggcgacg ttctcgagca cgaccgccgt cttgatgccc
      181 gcggcgcgcg tgcgatcagc ggcggaaccg gtcgtcggcg agatgtgcgc cacgatcttc
      241 gtcttgcgca tgtcctccga gtgcgcgcgc gacaccgccg cgacgcggtc gtcccacaac
      301 agagccggca agcccgccgc catgcggtcg cggttcatga gcgcgagcag gcgcttctcg
      361 gcttcctcgg cagagacgac gacgttgtca tcatgcgtcg gctcgatcgt gatcatcagc
      421 ggcggatcca tgccgcacca gaccgggaag ttcgcgagca ccgtcgagcc ggctgcatcg
      481 ctcgcagcga tctcgacctg ctgcttgccg ttgtgcttcg cacacgaaac gctcgccttg
      541 aacgcgccct gcttgttgac gtcgatcgga atgcgctcgg tcttgccgtc gtcgtgcgtg
      601 acgaacagct cggtgtcgcg atagcgaagg tcgacgaccg catcgacggt gaacgagccg
      661 cccgccgcca ccgaacgcgg aatcggcgcg gtcgcgacac cggacgcttg cagcgcgaac
      721 acgatcgcgc ccatgccatc gtccgtccgc ttcgcagtgc cgatgccgac gcgcgccatc
      781 gtgccttcct tcaagctctc gggcagcttc gccttgagct gctcgacgat cgactcggtg
      841 tcgtcgacgg cgccccacac gacgagcagc gacggtgacg gctcgatgat gccttggcgc
      901 tgcagcgcga attcgacgac gctgtaatcg agcagcccgt cctcgggcat gatccgcgcg
      961 agctcggcgc acgcgcgaaa tagccggacg tcgggagccg gcaccggcac cttcgacgcc
     1021 gtcgcggctt ccttcaccgc ggcaagcact gcgtcgccca gcggtgtcgg cgcaggcgcg
     1081 cgcagcggct cgttgtaacg aatcgccggc tcgctcgtcg gtgcgaacgt gaccgggcct
     1141 tgcttgctcg cgttgctacg ccagctcggc tgattgccga cacgccggct gccgctcgaa
     1201 cacgctgcga tcaagagcag caagagcgcg gcgcgtgtca tcgccgtcga tcgtatcacg
     1261 gcgatttcgg attgctcgtc gcggccggaa gcgtcggtgg cggcgtcgcc gtcctcggca
     1321 cggtcgtcgg cgccatcgtc ggcccggcgt gcggcgcatt cgcgggggcc ggtggcgcgg
     1381 tcggcgccgg aactttcgaa gccggagctt tcgaagccgg agccttcgaa gccggagcct
     1441 tcgaagccgg agccgtcgga gccggagccg tcggagccgt cgtcggtgcg ccgctgcctt
     1501 gcgagaccgc gctgccctgc gcgttgccct ggatcatcgc tggcgcgatc ggcgcaagca
     1561 tcggcgccgt ggccgagccg gtctgcggta ttggcgacaa cggcgcggcg ctgccggccg
     1621 aaccggtctg tggggccgcg ctaccagcgg aaccgccaga ccccgtgtct gcgcccggcg
     1681 gcttcggcac gatcgatcca ctcggatcgg cgaatacgac cgtcaacatc ttatcgagcg
     1741 catgaaagtc cgaatacgag atgcggttcc agctcgcgac accgggcgag ccgccattgc
     1801 tgcggtagtc gacgcgcacg acattgaggc gccaccgatc gccgacctcg ggcggcaagc
     1861 gcaccgccat cgtgtcgtgg cggcctttca cagcggcgag cggaatcgcg atctcgacat
     1921 cccagccttg atcggtgtcg cccgccttgt ccgcggtccc tcggaccttc accacggtct
     1981 ccatgttcgc gtcccacttc tcgtcgccgg gctgcgcgcg cgtcgtcgcg aagaacgagt
     2041 caaacgtcgc gttgttcggg ttgacctgca actcgacgta gcccgtgcga ttgccgtcgg
     2101 cgtcgatgaa cacctcgacg caatccgctt tccacagcgg ctcgtcgtgg ttcttgtact
     2161 ggctgtacac gtcgctatcg acgatagaga cgaacagata caagttttgc tcgtcccacg
     2221 tcatgcgcgc ggtcgccttg ccgaccggat cagcgctgcc gtcggcagtg atcagctccg
     2281 gcgacgtcgc cgcgctcgac cagccgggct cggtcgccat gccgtcgatc gtgatcggtc
     2341 cggtcgcgtg cgggatgtac accgtgccct ggggcggcgg cgcctttgat agatcgacgt
     2401 cgatcgtgcg cgcgacgatc gcgcgatcga tgacgttgcc accggtcgcc ggcatgcgat
     2461 cgccgatctg gtgcttgccg tcctcgacga ggccgacata cagcgtgacc gtcggcgatc
     2521 gccaatccgg gcgcagcacg atgtcctgca cgtcctggat gatctggccc gccttccact
     2581 tcttcgtcgg gtacccgatc tccatgtccg tcggcccgaa gttcatgaag tccgcgctgc
     2641 cctgcgcgcc gcgcgcatac gcgaacacgc gccactgctc gcccggcggc atgttcacgc
     2701 gccagtagtg catcacgcga atcgccgcgc cgggcgcgag cggcgtcttc ggatccttgc
     2761 cgtcgacgta gacgaccttg ttgccgaggt acgtcacctt gccgccgaga tcgacctgca
     2821 tcggctggat gtcttgcggc accgccgtca gcaagttctc gcggatgtag ctcgcctcga
     2881 tgcgcttgcc ctgcggaccc gacccgtgat cgacacagcc cgcgagcacg atggcgatga
     2941 gagcgatgcg cttcatacga tcagatccga tgcgtcctgg aacatttgct ggttcgcttg
     3001 ctggcgcgtc ttcgcgagct tctcgacgat cgcgagcacg gtcggatacc gcttcacgac
     3061 cgcatcgaag tctttcttcg cgagcttgag caacacgagc ggctcgagcg ctgtgacatc
     3121 cgcgccagcc gctgcgccac tcacgagcga catctcgccc accgccgagc ctggctgcag
     3181 ctccgccaca ggcgcgccac cgctcgacac ccgggcggtg ccggacacga tcacccacag
     3241 cgcgtcgttt gcctcgccat cgcggatgaa cgtcatgccc tgcggaatga gctcgctcga
     3301 gaacttgccg agcaactctt cgcgcacgtt ctcgtcgagc ggcttgaaca gctcggacgt
     3361 gcgcagcacg ttcgacagca gccgcgcccg cgcgtggaac gcgatcactt cggcgagctg
     3421 cggatgtttc tgcgccgccg tctccagcgc gcgcgccgga atctcgagca gccagacgtc
     3481 ttcggccgcg atcacgttcg cagtgcgctt cgtgccgccg accagcgcga tctcgccgaa
     3541 gaacgcaccg gagtgcagct cgccaagctg cgcgatcggt cccgattgtg cgctcgcggg
     3601 ctgaaacttc ggccgcgaca ccgtcacggt gccgtgcgcg atccagtaca gctgggtcgc
     3661 cggcgcgccg atgtcgatga tcggtgtcat cgccggatac gccttcgcgg tcatcacacc
     3721 gacgagcgca cgcgcgcccg gcttcgacag cgccgacaac agcggcgccg gtgggacctg
     3781 cgttggctgc cgcttcatga gccacgcacc gagctcttcg atcaccgcgc gcgcctgggc
     3841 gagcggctcg cccggcggcc aatcgacagc cggcggatcc tcgggcgacg gcggcgtggt
     3901 gaacgcgaag tgcggtccgc cggtcgcgta cgtatcgatc acctgctcga tcagctccgg
     3961 cccgcggacg cttccgagct cggccaggtg ccggccacac gcgaccgcca gcgcgacgcg
     4021 tccggtgaca cgtgcgccct cgccgatcgt ggcaaacgcc agctcgactt catccgtggc
     4081 gccgagcgcg ccgaggaccg cggcgacttc gagccacgcg ccccaatcgg tgggcgccag
     4141 gcgtagtttc tgcgtcgcgc ggacgaggtc gtcggccgtg accgtcacgg accccgttat
     4201 atcgcggttc cgatcacatg ggcggaggcc aacgaactac gtggatttcg cgttcgtgcc
     4261 acggaactag ggatgatcc
//
LOCUS       DASUFX010000441         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1877043, whole genome shotgun sequence.
ACCESSION   DASUFX010000441 DASUFX010000000
VERSION     DASUFX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877043"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFV99_17610"
     CDS             <1..731
                     /locus_tag="VFV99_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061190.1"
                     /translation="LGRGGSDTTAVALAGALRADYCEICSDVEGVFSADPRVVDAAQL
                     LHSISHDEMLELAAQGAKVLHDASVEFARKSGIALYARATNKDGGGTRIDFTPERERV
                     VAAVTGQSSLIRVRVSGGAAVEHCLQIIEGASIPLTHLDLEGKELRCWFTIADVPDWG
                     EVRKRLDTVLGDQIEFGEEGCVTMVGHGIGGNPGIINKARASAVAAGVPLNAVSVSPL
                     RVTLWCDRPHVDNLVRALHKTFIE"
     gene            734..1534
                     /locus_tag="VFV99_17615"
     CDS             734..1534
                     /locus_tag="VFV99_17615"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5061191.1"
                     /translation="MAKSPPLAVLSRFVPHLGRLGRAERWRRMRGRPRIWAHRGASAH
                     APENTLEAFELARTAGADGIELDVRLDRDGTVVVFHDRDLERLCGRPGRMEDLSADER
                     KQLRVSGTAAVPTLEEVFHMLGDLELNVEIKSNNPGRNGDLVVATANVIRQSGRADQV
                     LVSSFDPFSLLTFYRQLPDIALAFLFGADQTLPIRKGWLARAMGATVLHPEHTLCTPE
                     NVKAWHTSGRPINTWTVDDEGELRRLAELGVDGIFTNDPAKALSVLSS"
     gene            1566..3173
                     /locus_tag="VFV99_17620"
     CDS             1566..3173
                     /locus_tag="VFV99_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061192.1"
                     /translation="MGRFWIVVVTLAACGDNGSAPKDAAGDAANDAAVDAATCETLGC
                     EDNETCTNDVCDPVDGCRHVSLDRCAEPLCAMTSCAGTDTDGDGLQDAWELQGYVDVN
                     CNGVNDGDGVDVQLADANVAVPDAYIEIDYHEMAGTGAACTNDAAGDAACNSAVLGES
                     CVNGVCTHTHRPRQASLDLARLAAARGLSPLPNDPSPPADLAGKPGFRIHWEHADAIP
                     ETSGLVVSFGNYEASRDLDPACVGPNGQNFFDLKDAHFFGATNPALFAARRRIVHYAI
                     ATHVSQCPIGAVLPNDFCQACPADRGGGTPQAGSSGSAEIPGNDFMISLGDRFFTLGR
                     LTTVIPREEAGTILHELGHNLGLLHGVLLVNGVHTPSFAPEYNPIYFSVMNYNYQFGM
                     LFAATPGSKVPVSFRLDYADDTVCAELSEASLIESDGAQCGPTSTRVIRFVPNTGTPV
                     FGSATTGTGIDWDNDGTISTTAGPRDLNGIVANETFYTINEWGYAAGKFTNLAFDSFC
                     SPWAMADALPSQEARVFEPSSRLTQRR"
     gene            complement(3180..3836)
                     /locus_tag="VFV99_17625"
     CDS             complement(3180..3836)
                     /locus_tag="VFV99_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061193.1"
                     /translation="MRALLCCALLAACGPKVAPTGSAFDEDLPRERTAEAAPTPVEKP
                     RPEAPPGKGLRTGTIERAKLIAVLDGGPGAFLRQLEVTPRMDGDRFVGWQLVQLLDRK
                     GPLVDVDVAPGDVLLAVNGRMLSRPDQLQSVWDSLRTANELRAQLWRGDNKLELTFAI
                     EPKVDPSTLPPPATTKVEPPPAKVEPAKPAPSKPDAAKPAPAKPSPVKEAPASKQPAK
                     "
     gene            complement(3876..4484)
                     /gene="tmk"
                     /locus_tag="VFV99_17630"
     CDS             complement(3876..4484)
                     /gene="tmk"
                     /locus_tag="VFV99_17630"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5061194.1"
                     /translation="MAGRLIVIEGLDGAGTTTQVRRLVEHLNAHGKPAHGTREPSDGP
                     VGKLIREMLTGAHAIAGQKLSQGTFGLLFAADRLDHMQREVEPKLAAGATVVSDRWYH
                     SSLAYQGTGADRDWIAMLNGRALRPDLTIFLSVRPEIAAERRAAARRKEELFEDLRMQ
                     QEVDAGYRATIAELKAAGERIETLDGEQTEDQVFAAILRLVG"
BASE COUNT          744 a         1583 c         1514 g          754 t
ORIGIN      
        1 cgctcggtcg cggtggctcc gacacgaccg ccgtcgcact tgccggcgcg ctgcgcgccg
       61 actactgcga aatctgctcg gacgtcgagg gcgtgttcag cgctgacccg cgcgtcgtcg
      121 acgccgccca gctcctccac tcgatcagcc acgacgagat gctcgagctc gccgcgcaag
      181 gcgcgaaggt gctccacgac gcctccgtcg aattcgcacg caagtcgggc atcgcgctct
      241 acgcccgcgc caccaacaag gacggcggcg gtacgcgcat cgacttcacg ccggagcgcg
      301 agcgcgtcgt cgccgcggtc accggtcagt cgtcgctcat ccgcgtgcgc gtctcgggcg
      361 gcgccgccgt cgagcactgt ctgcagatca tcgagggtgc ctccatcccg ctcacccacc
      421 tcgacctcga gggcaaggag ctgcgctgct ggttcacgat cgcggacgtg cccgactggg
      481 gcgaggtccg caagcgcctc gacaccgtgc tcggcgatca gatcgagttc ggcgaagaag
      541 gctgcgtgac aatggtcggt cacggcatcg gcggcaatcc cggcatcatc aacaaggcgc
      601 gcgcgagtgc ggtcgcggcc ggcgtgccgc tcaacgcggt cagcgtgtcg ccgctgcgcg
      661 tgacgctgtg gtgcgaccgt ccgcacgtcg acaacctcgt tcgcgccctg cacaagacgt
      721 tcatcgagta gcaatggcta agtctccgcc cttggcggtg ctgtcgcgat tcgtgccgca
      781 cctcggccga ctcggacgtg ccgaacgctg gcgccgcatg cgcggtcggc cgcggatctg
      841 ggcccatcgc ggtgcctcgg cgcacgcgcc cgaaaacacg ctcgaggcat tcgagctcgc
      901 acgcaccgca ggtgccgacg gcatcgagct cgacgttcgt ctcgatcgcg acggcacggt
      961 ggtcgtgttt cacgatcgcg acctcgagcg gctgtgcggc cgaccgggca ggatggaaga
     1021 cctgtctgcc gacgagcgca agcagctgcg cgtcagcggc accgccgccg tgccgaccct
     1081 cgaagaagtc tttcacatgc tcggcgacct cgagctcaac gtcgagatca agtcgaacaa
     1141 ccccgggcgc aacggtgacc tcgtcgtcgc gactgcgaac gtgatccgac agagcgggcg
     1201 cgccgaccag gtcctggtct cgtcgttcga tccgttctcg ctcctcacgt tctatcgcca
     1261 actgcccgac atcgcgctcg cgttcttgtt cggcgccgat cagacgttgc cgatccgcaa
     1321 gggctggctc gcgcgcgcga tgggcgcgac ggtgctgcat cccgagcaca cgctgtgcac
     1381 gccggagaac gtgaaggcgt ggcacacgtc cggtcgcccg atcaacacgt ggacagtcga
     1441 cgacgaaggc gagctgcgcc gtctcgccga gctcggcgtc gatggcatct tcacgaacga
     1501 cccagcgaaa gcgctgagcg tgctttcgtc ctgacgcttc tgcaccgatc gagtacgctc
     1561 gcgggatggg tcggttctgg atcgtggtcg tgacgctggc ggcgtgcggc gacaacggca
     1621 gcgcgcctaa ggatgcggcg ggcgatgcag caaacgatgc tgccgtcgac gcagcaacct
     1681 gcgagacgct cggctgcgag gataatgaaa cgtgcacgaa cgacgtctgc gatccggtcg
     1741 acggctgccg tcacgtctcg ctcgatcgtt gtgccgagcc actgtgcgcg atgacaagct
     1801 gcgcgggaac cgacaccgac ggcgatgggc tgcaagatgc ctgggagctg cagggctacg
     1861 tcgacgtcaa ctgcaacggc gtcaacgatg gcgatggcgt cgacgtgcag cttgccgatg
     1921 cgaacgtcgc cgtgccagac gcgtacatcg agatcgacta tcacgagatg gccggcaccg
     1981 gcgcggcgtg cacgaacgat gcggccggcg acgctgcgtg caactcggcg gtcctcggcg
     2041 agagttgcgt caacggcgtg tgcacgcaca cgcaccggcc acgccaggca tcgctcgacc
     2101 tcgcgcggct cgccgcggcg cgtggcctat caccgctgcc caacgatccg tcgccgcccg
     2161 ccgacctcgc aggcaaacca gggtttcgca tccactggga gcatgcggac gcgattcccg
     2221 aaacgagcgg tctcgtggtc agcttcggca actacgaggc gtcgcgggat ctcgatcccg
     2281 cgtgtgtcgg gccgaacggg cagaacttct tcgacctcaa ggacgcgcac ttcttcggtg
     2341 cgacgaatcc ggcgttgttc gccgcgcgcc ggcgcatcgt tcactacgcg atcgcgacgc
     2401 acgtcagcca atgcccgatc ggagcagtct tgcctaacga cttctgccaa gcctgcccgg
     2461 cagatcgcgg tggtggtacg ccgcaggctg ggtcatccgg cagcgccgag atccccggca
     2521 acgacttcat gatctcgctc ggtgatcgtt tcttcactct cggccgactg acgaccgtca
     2581 ttccgcgcga ggaggcgggc accatcctcc acgagctcgg gcacaacctc gggctacttc
     2641 acggcgtctt gctcgtcaac ggtgtgcaca cgccgtcgtt cgctcccgaa tacaacccga
     2701 tctacttcag cgtgatgaac tacaactatc agttcgggat gctgttcgct gcgacgcccg
     2761 gaagcaaggt cccggtgtcg tttcgactcg actacgccga cgacaccgtg tgcgcagagc
     2821 tgtccgaagc gtcgctgatc gaatccgacg gcgcgcagtg cggtccgaca tcgacgcgtg
     2881 tcattcgctt cgtcccaaac accggtaccc cagtgttcgg gtcggcgacg actggcacag
     2941 gaatcgactg ggataacgac ggcacgatca gcaccaccgc aggcccgcgc gacctcaacg
     3001 gcatcgtcgc gaacgagacg ttctacacga tcaacgagtg ggggtacgcg gccggcaagt
     3061 tcacgaacct ggcgttcgac agcttctgct cgccgtgggc gatggcggat gccttgccat
     3121 cgcaggaagc gcgcgtgttc gagccatcat cgcgactcac acagcgtcgc tgactgcgcc
     3181 tacttcgcgg gctgcttgct tgccggcgct tccttcaccg gcgatggctt cgcgggtgca
     3241 ggcttcgcgg cgtcgggctt cgacggcgct ggcttcgccg gctcgacctt cgccggtggc
     3301 ggctcgacct tcgtcgtggc cggcggtggc agcgtcgacg gatcgacctt cggctcgatc
     3361 gcgaacgtga gctcgagctt gttgtcgccg cgccagagct gagcgcgcag ctcgttcgcg
     3421 gtgcgcagcg aatcccagac cgactgcagc tgatcgggtc gcgacagcat gcggccgttg
     3481 accgcgagca gcacgtcgcc cggcgcgacg tcgacgtcga cgagcggacc tttacgatcg
     3541 agcagctgga cgagctgcca gccgacaaag cgatcgccgt ccatgcgcgg cgtgacctcg
     3601 agctggcgca agaacgcgcc tggtccacca tcgagaaccg cgatcagctt cgcgcgttcg
     3661 atcgtgcccg tgcgcagccc cttgccgggc ggtgcttcgg ggcgcggctt ctcgaccggc
     3721 gtcggcgccg cttcggcggt gcgctcgcgc ggcaggtcct cgtcgaacgc gctgccggtc
     3781 ggcgcgactt tcggaccgca ggcggcgagc agggcacagc agagcagggc gcgcatcccc
     3841 tccaagctgc cgctcgggcg agcccgacgc aagtttcagc caacgaggcg caggatcgca
     3901 gcgaagacct ggtcttccgt ctgctcgccg tcgagcgtct cgatgcgttc gccggcggcc
     3961 ttgagctcgg caatcgtcgc acggtagccc gcgtcgacct cttgctgcat gcgcaggtcc
     4021 tcgaagagct cttctttgcg gcgggcggcg gcgcggcgct cggcggcgat ttcgggccgc
     4081 acgctgagga agatcgtgag atcgggacgc agcgcgcgcc cgttgagcat cgcgatccaa
     4141 tcgcgatccg cacccgtgcc ttgataggcg agcgacgagt ggtaccagcg atcggagacg
     4201 accgtcgcgc ccgcggcgag cttcggctcg acttcgcgtt gcatatgatc gagacgatcg
     4261 gcggcgaaca ggagaccgaa cgtgccttgc gacagcttct ggcccgcaat cgcgtgagcg
     4321 ccggtgagca tctcgcggat cagcttgccg accggtccat cgctcggttc gcgcgtgccg
     4381 tgcgcgggct tgccgtgcgc gttcaggtgc tcgaccagcc gacgcacctg cgtcgtggtg
     4441 ccagcaccgt cgagaccttc aataacgatc agccgtccag ccacatcgtc gtcttaccac
     4501 gacgacgaac gacattcgtc tcgccggtgg cgacgaaacg cgggctacgc gacgcggcgg
     4561 cgccggtaca tcgccagccc gaacgtcacc gccga
//
LOCUS       DASUFX010000442        24575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1183216, whole genome shotgun sequence.
ACCESSION   DASUFX010000442 DASUFX010000000
VERSION     DASUFX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183216"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..422)
                     /locus_tag="VFV99_17635"
     CDS             complement(93..422)
                     /locus_tag="VFV99_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061195.1"
                     /translation="MAELRPCTECRRHVAIDERACPFCNAELTVAAPSSTRLGRVSRA
                     AVFAGAALATTACGGKKPKTDTNVQDHEQTASGDAGVEQPPPPPPDDKNNVPMPYGAP
                     PARKRVV"
     gene            complement(433..786)
                     /locus_tag="VFV99_17640"
     CDS             complement(433..786)
                     /locus_tag="VFV99_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061196.1"
                     /translation="MAELRPCAECRRHVSIDERVCPFCSAELIAATPTRVVLGRASRA
                     AVFASAALATTACGPPKRTNIPPAQQLPDAGVRDAPTGTTYETPPDAKTGQAEPEPSP
                     VKMPYGAPPARRRVV"
     gene            complement(799..1263)
                     /locus_tag="VFV99_17645"
     CDS             complement(799..1263)
                     /locus_tag="VFV99_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061197.1"
                     /translation="MTALVPCPACKRHVATRERACPFCAHVLPVARAQHITVIGRVTR
                     AAVFSAALVACGKDKPKEAPAPPPAQGSDDLEKMLDEPRTVEHAAVPPPVDAAVIEPD
                     AAVVDAGAPPDAGVVKKKHVQKQQVQDVRDIKIDHIQNAKPYGAPPARRRIV"
     gene            complement(1260..1712)
                     /locus_tag="VFV99_17650"
     CDS             complement(1260..1712)
                     /locus_tag="VFV99_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061198.1"
                     /translation="MTALAPCPACKRHVATHERTCPFCARALPRMRAQHVTVIGRVTR
                     AAVFSAALAACGKDKPKEAPPPPPAQGSDELEKMLDNDGKTVEHAPVPPVDAAVIEPD
                     AAIVDAGLPPDAGVVKKKKPHHEQAVPLPPPHNIPKPYGAPPARKRIV"
     gene            complement(1709..2278)
                     /locus_tag="VFV99_17655"
     CDS             complement(1709..2278)
                     /locus_tag="VFV99_17655"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5061199.1"
                     /translation="MRLRPCEGCRRHVAVDEARCPFCGHALAPVAMGLLVPRGRFSRA
                     AVFAGATLAGACWTGSTTPRETPIEHKQVESKPSVPPPGTIRGVVHNADGGAPLPGVT
                     VTVQKPDGSPVYATTNAKGEYEFKNLPPGQYAVMFQSGNPNETPQGTPVTLESDAGAT
                     ADVFIDLTIPDRAPCCKPYGAPPARRRIV"
     gene            2474..2944
                     /locus_tag="VFV99_17660"
     CDS             2474..2944
                     /locus_tag="VFV99_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061200.1"
                     /translation="MARRDHDDLDRALRAILDPKTAAREAANLLDVFRLALAVHLVAE
                     TRVHSTLLALVRPPAALRSQIAQLRREHDVQQETAERLAELEPCSDAWYCGALELRVH
                     VLDHAKREDYFRAALDDYVPSAVSRGLGAEYATERMKLLATTSPLAVARYINVA"
     gene            complement(2960..3574)
                     /locus_tag="VFV99_17665"
     CDS             complement(2960..3574)
                     /locus_tag="VFV99_17665"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5061201.1"
                     /translation="MTLRACSQCRRHFAAEPACPFCGAAAASPPPARVTVGGRLSRAA
                     AFAALAGCYTSNPPPQYGPPPPPPPSHEQQQQQQQITEDQQKFAKPPDAGTSAIQGTL
                     KNSQSAAPLANWPVQLQSPSQPSMQPRNAQTDANGHYAFVDLPAGDYVLVFGYDNHPR
                     RRAPRQNVTLGDHDAKTVDQQIFIPPPSNIPMPYGAPPARGRVV"
     gene            complement(3579..4718)
                     /locus_tag="VFV99_17670"
     CDS             complement(3579..4718)
                     /locus_tag="VFV99_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5061202.1"
                     /translation="MTTPLPRRAYLGAELPGDREAFSPEGVRIAGVIEGTMAARADLR
                     AGDVLVSIDGHIVHDLRTLAAALRIAGHAATVELIVLRDGVHHVTRTDVVAVPLEPDA
                     RYGALDVGDARLRTILTCAQTPRALVLVIQGIACESIDFALDPDAPLGALVAGWAQAG
                     YDALRFDKRGVGDSEGPPCAETDFHTELADARAALELARETAAQRNVPLVVFGHSVGG
                     IIASQLARNVAGVIVYGTPVMRWLDCLLDSTERQLRMRGATSAEIAAQLAAIQNLAKT
                     GELNGRSAAYHEQLHALDLEAAWREVDAPVLVVRGEHDWVVRPDDQSRIADLAPGTTS
                     VIDLPGLDHLFGWHAGRVASLRDYGSGRADASLVDATVAWLDRLR"
     gene            4804..7503
                     /locus_tag="VFV99_17675"
     CDS             4804..7503
                     /locus_tag="VFV99_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061203.1"
                     /translation="MGSSETLRSVIDAGRSSKRLDELSSARVIGKIAQQIHAAQQKAG
                     AGKAVGPITPANIKLAANGETKLELGASAGSLGYSSPEQTMGGFGDRRSDVFSLGTVL
                     WEALTYQRLFDAMNDAAVRMAVQEREISPPSEVNANIPAELSAICMRALNRNSTDRYQ
                     SLKAMAVEIEEFLEEAGYADDDSKIAAYLATMNDAPKAVKIEMAPLARPASSTQPPPS
                     TLTPPSTTKPPVEKSASGTQPPANVMKPPAAPSMLGEAPKSTPAKPAGHALAAALANA
                     GATPATSNGSTTTAAASTTGTSLGLPPINPSTTAVGVPKADDWQDSSKKADAPLSPLP
                     PVLPSGSTPLRNDRSTIAVSVPPQVKVATVEAKHAEAKQPAMIVDDKPAAQIVEDVKA
                     TIVDSKPPAMIVDDGKATLVDSKPPEIVVPKLVDGKLPSAVEQKPEPVKQSAVETKPV
                     AAVTPAAAETKPAVETKPADTKRAASEAEKKPEGRKRAPSQAGGRKRPPSQGELPQAA
                     AAVSLPGGGRDSKDVLAGWGWGTDKHNAIAPDDYPHDDDYDFHEPNSRKMLVKIIGAG
                     MGVVALIIIVAFAFGGSSDKKKTSAAKQADTTKPLVEQPPAPPPEAAPVAQPAAGSAD
                     PAPGSADGSAVAAAQPTEPAQTEEQKAEAAKAEAAAAEAAKAEAAKAEADRIAAEKEQ
                     AKQAEVAKAEAAKQEAARIAAEKEQAKQADAAKKETAKQEAARIAAEKKAEAARVAAE
                     KAEAKKAEQARIAEAKKAQAEARKAEAARIAAEKAEAKKTQAAAAKTTPKARPEKKQP
                     KAETKVAKAEPKTETKVDGDTAYRQGLQLFARGDTSGALASLRTALAANPNHAPTWRG
                     LGLVFEKMGEKDQARVAYKRYLQLAPGAGDAEQIKGRLERLQP"
     gene            7500..8510
                     /locus_tag="VFV99_17680"
     CDS             7500..8510
                     /locus_tag="VFV99_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061204.1"
                     /translation="MKRVFVIALLCLAVGRYHAASAGPRQVLVLKSEGTADASSRTTI
                     DTHVLRIAKNIDGNVVAGDITLTEAAALVGCSSSEAACKDQVLDTLGVDEVVASTVTA
                     TPTGLNVTVRRIAKGTPPKAAQTTIPSGKTPDAKLNADIGPLFGLTAPAVVEPPVTAP
                     PVTAPPPPPPPTTTPTTTSPTTTPATTTPTTPPRPVTADASTAPMNDVTAAPNGVVTQ
                     QSEGVPRRRWQKIGMGVGGGLVLLGFVMWAQAASVQGQIDDKPEPVSPRDFQDLRDLE
                     KKGDDLASGGNFFFVVGALVGGVSTYYYVKAGRHRPQTARITPMAVPGGAGVTLTLGG
                     AP"
     gene            8507..9154
                     /locus_tag="VFV99_17685"
     CDS             8507..9154
                     /locus_tag="VFV99_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061205.1"
                     /translation="MKLVVAGMFVVVVALITASSTSCSIHHRSDQYACTVNGDCDGNR
                     ICDNGFCIAPGTVDAPRTIDAPKPPGDGSNGCPSQCTTCNVTQKTCTINCAMGANCTN
                     QVVCPAGYKCDIQCNTDNSCRNGVNCQQAASCNVDCIGKQSCQTVVCGPGPCDVGCSG
                     PASCRGVACGSSCACDVLCTGNQSCAEGILCTSLACRSATGSGCTSVPTFCHSCN"
     gene            complement(9151..10782)
                     /locus_tag="VFV99_17690"
     CDS             complement(9151..10782)
                     /locus_tag="VFV99_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX5061206.1"
                     /translation="MRFSRLALALAFGASPAWAQPAAGEPPAETPKDPPKESPKGSAD
                     EKPVEPQSSKPVPIEVPQVRTVTQPEVSPTAEKAEEEDLDLDNYHAKDRGFRFGSYGR
                     VLAGSDLRGGKPQKVSIVAHGPRIVEDSYLELEFSYGFERQAGSGGANSYIIVRPIVT
                     LAFAGTLFHDTGQFDAQPALRNMFLDGQVGDHLTLWVGSRMYRGDDIYLFDYWPLDDI
                     NTVGGGLMYKRDIMLRHDADGAATADSVEIAGHIGFNRLNNPFQFQEVEVANPVQGAT
                     TVEQLNRQRTVASASAAYVMMPTGAGIGAKLKVHGEFHSLPSGTFKRMDGTLQALPSD
                     TGFLIGAELGAFGFSDDSRFRRHLNLFTRYAKGLAAFDELAPPTSFGADLKTGRANEF
                     TFGISGNWDTKFANMMVGVLSRRFVDADEDTVDSDDGWEYAVDARPLVRVPTTDFFVG
                     GDISYQSRFPRGLNNITLKAEDPAVFQIAPMLTWSPMGPSSYDRPQIRLVYRAAYLND
                     AALDQYVPDDPRHSHKLVHFLGLQAEWWFNSSTYR"
     gene            10994..12856
                     /locus_tag="VFV99_17695"
     CDS             10994..12856
                     /locus_tag="VFV99_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5061207.1"
                     /translation="MFVLVGCPSSQDPNLTNGDAGTGDGGGDAGPSCPVVPSCTTTIQ
                     YKGAGTAVSLRGDFASDGWTTGIAMTKNGDTWEATVPVRDNQVIVYKFVVDGNWIADP
                     ENPRKSPDGYGAYNSVVRADCDQCPPRAAVDWRDAIMYFVMIDRFADGDMANNAPVAG
                     VELPGQYQGGDFVGLTKKINEGYFGQLGINTLWVTSPLDNADNANPGSDGHSYSGYHG
                     YWPRDLDSAESKLGTEAELKAMVDAAHAHGIQVLIDYVMNHVHTQSPIYAQHPNWFWP
                     NDNGFGGNCVCGAGCSWDTDRLKCWFDPFLPDFDFRNPDARKYSVDNAVAWAKRIGID
                     GFRLDAVKHIETSWLTDVRARLNAEVAFDQKFYMVGETFDGDRGIIKSYVNPSTMLDG
                     QFDFPLRGQVLATLLRRDGQMSDLAGFLDANDGYYGQGSVMSTFLGNHDVPRAIELAL
                     DTPMFGAWDGGKNLAWSGQPQLPQSKNPFERLAVAYTLLFTMPGIPMLYYGDEFGMPG
                     AGDPDNRRFMQWSDYTANQTWLRDRLAALAKMRGEHIATRRGTRQTLGVSGDALVYKM
                     TSSGDTVFVALNRSDNAQTAPNLPAGNYVDLISGAAVTAPLTLQPRSAVVLSQQ"
     gene            complement(12862..13512)
                     /locus_tag="VFV99_17700"
     CDS             complement(12862..13512)
                     /locus_tag="VFV99_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061208.1"
                     /translation="MFAGIPRWELITVWVLRIALLATCIALFAEQEWATAVTCFVAIA
                     LVVTPTIISRTGHFIWPIEVELALLVLVTAHMSLGYLFGLYLRVDVFDKVLHLVDSLL
                     IGFVAFLAVYLAHFIRGDRSHHWLDAIFIFLITLGLGALWEICEFASDQLFGTYAQGS
                     PTMPPLLDTMWDLILDGTGGVLAAVLGPIYMKHSRRSRVRVRTFAERVEHHRYVAT"
     gene            13674..14165
                     /locus_tag="VFV99_17705"
     CDS             13674..14165
                     /locus_tag="VFV99_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5061209.1"
                     /translation="MEVQRCRIYTGTGELQSTRTRPMERPLVPRTPTIADRISISAIM
                     TRDIVCARPELSIPALKSLLVQNHIGCVPIVDDRGRPKGIVTKLDLVEAKQPALTAAD
                     VMMPLAITLNSHATIAHAAAMMAIEDFHHLMVVGNDHILVGVVSTMDIVRWLAKNDGF
                     TGA"
     gene            14185..14724
                     /locus_tag="VFV99_17710"
     CDS             14185..14724
                     /locus_tag="VFV99_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061210.1"
                     /translation="MRLWFALLLALANCHPSPSKPAPGEVPPLPPASGTAVGYLVDAA
                     GELQLRDDQVTKLKDIDTSLAAENGQIDAQLRQIEKPVPAEEVSPQDQKAGVKPQRYN
                     NAPGASTITTTDSQKLRKMRDDNDRDALIKAFKVLDAEQLTKAKQILADRGIAIPGEA
                     KQEQTHDPEDGKPLPGMEP"
     gene            14766..16787
                     /locus_tag="VFV99_17715"
     CDS             14766..16787
                     /locus_tag="VFV99_17715"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX5061211.1"
                     /translation="MRTSKLLPLMFIAACGGPKKQPAPPAPAPEPTAGAPAVAPVPTP
                     APPPGPNDAALVAEATEFAKHADEELRKLYVDSSVADWANQTDITPEHEAASAKASEA
                     LANGLTKLIKESRKFERVLPKLDPDTRRQLWLLKIAGQPAPDDPAQAEQLAKIAAEMT
                     SIYGKGKVCDASKPKAGAPKQAQLDDLAAKLKAEQDPKKQDALKKDLAAKQKKFDQAY
                     CKDLDALSKVLQKSRKPDELLAAWKGWNDNVGKAIRPLFTRYVEIANAGARGVGFKDV
                     AEMWKSGYDMPPDQFEKEVDRLWNQVRPLYVQLHCYTRRGLNKLYGDNVVSKTGPIPS
                     HLTGNMWAQAWGYLYKELQPYPGVAPIDVTPTLEKKFDYTKMVKMAEGFYMSLGMNPL
                     PKTFWERSMFQKPKGKDVVCHASAWDVTLNNDLRIKMCINKNQEDLVTIHHELGHNYY
                     YNNYYTLPALYQNGANDGFHEAIGDTIALSMTPEYMKQKGLLTKVEKNEKATINQQMD
                     VALEKIAFLPFGLLVDKWRWDVFSGKVKPDEYNKHWWDLKLKYQGVAPPIARTEAEFD
                     PGAKYHIPANTPYMRYFLAAVLQFQFHRALCQKAGFKGPLNECSIYGNKEAGEAFKKM
                     LAMGASKPWQDALFELTGQREMDATAILEYFAPLQKWLEEQNKGQQCGW"
     gene            complement(16788..17387)
                     /locus_tag="VFV99_17720"
     CDS             complement(16788..17387)
                     /locus_tag="VFV99_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061212.1"
                     /translation="MRLLLVLLCSCSWVFTEGPKPETADQPCNASEASPFVDAAFAVV
                     YGITAFVFVADMDSSGDNSDQVKGAVVSGLSAGLLGLSARSGLINVRACRRHNNELYE
                     QQKAEAAAAAAPRVSPSRRDAWQLTKQAAIAARNGDCATVMALEQVVLGLDTEFHGAV
                     FMRDAAIVHCMQTTEVPVQPPGETPPAPAPVTPPLVPTP"
     gene            complement(17429..18016)
                     /locus_tag="VFV99_17725"
     CDS             complement(17429..18016)
                     /locus_tag="VFV99_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061213.1"
                     /translation="MRLLLVLLCGCSLIFTEGPKPETPDKPCNASEAAPVADATFAVL
                     YGIAAFAFVADMDSSSDNGDQVTGVVITGLVAGLFGLSARSGLINVSACRRHNNELYE
                     QQQAAAAAPPEIPRARRDAWQLTKQAAIAARNGDCATVAALEQVVLGLDTEFHGTVFM
                     RDAAIVHCMQTTEVPVQPPGETPPAPAPVAPVTMP"
     gene            18270..19346
                     /locus_tag="VFV99_17730"
     CDS             18270..19346
                     /locus_tag="VFV99_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061214.1"
                     /translation="MKSFKNISFAFALALGAVTVATGCAADSPEQGGGGGGGGGGDGS
                     GSDDAPRPMDASGKYSVVSTFDIATNAPGKVGEVTNAFIAMTDGAADPAEWIIEQIIA
                     KTSNSTLKSILTSAKPFVAGYLNDRLLEIAPDFVTTIVQVGNDFGQMAKNFGLNEQLD
                     VSGSGMAYTSTVSALGVHFKVDNVESDIMFADHSLAAVTAANVAITLDATGKLDIGEH
                     KLPLSYGKVLRLGLDEVIIPAVDPTATNLNTLLAHLVDCSAVGTAINDALVDQFGFGG
                     GASTWASACTAGLGLGSQAIYTKIDSIDSSALEFGLVGTAKAVDSNKDSKVDTIQTGK
                     WTGTLSYGNSPSPLAAATFNGTRM"
     gene            19633..20649
                     /locus_tag="VFV99_17735"
     CDS             19633..20649
                     /locus_tag="VFV99_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061215.1"
                     /translation="MTSLLALGVTAAGCAADAPNVGDDDDGGGSGSGSGSETVPLTAE
                     GKYKMTSTFDVATNMPGTAGAVINQIIDATDSADDPTHWILDQLVAQLPSGSFKNTVQ
                     QAIPFVSGYLNDRLLDVAPDFVVTIRDMGNKFGQIARNFGTLETLEVGANGQATKTVT
                     GVHFVVDQLELDYAFADYGVQPVSVPSVVVQLEPTGRLSIGEHKVPLAYGKVLRIALD
                     EMVIPMIDPSAVTLEDVLKNVVNCHAVGQYTYDALGIGSPSTFESACLGGLKAGANAI
                     YNQMGKVDSVALEFGLTGIAKGVDKNHDGKLDQIQTGAWTGTLAYGGAGAPLAAAKFV
                     GERM"
     gene            complement(20652..21593)
                     /locus_tag="VFV99_17740"
     CDS             complement(20652..21593)
                     /locus_tag="VFV99_17740"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5061216.1"
                     /translation="MAVKLVRQWSHWVRTSETVLARWLRAAHAALLGEMPILAAGTAL
                     FAILAIVPTLAAAVAIYGLVADPTKIQPQIENLQHVLPVEVVHFIGLQLKRQASRGSG
                     ELSLQLVTSIVLAVFSSRASARSLIDALNRAYRVRERRKPIVRLGLSVTMALSTLIGI
                     ITMFAVLIVLPALVGAINRNWIPIAMLLRWPALLAIVFLTLLALYRYGPSPRPLGPEK
                     HTDAGGVVGLPPAPLRHICLPGAVIATCLLLLVSYGLSVYVDYVSSSELIRGAFGSVI
                     VTLLWFYLSTIALVIGGFVNAELERHRGAPEPERSMY"
     gene            21615..22391
                     /locus_tag="VFV99_17745"
     CDS             21615..22391
                     /locus_tag="VFV99_17745"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5061217.1"
                     /translation="MIDTHCHLDTAAFDADRDAVLARAAAAGITGILVPAIRPATWQP
                     LIDLARQRSLLRIALGVHPQIVPELAEGEVPANFADALARAIEDSGAVAVGECGLDGG
                     TADQPRQEELFRAHIRAARATKRPLVIHVLRAHDAAPRILREERADEVGGVMHSYSGG
                     AHLVPIYRAMNLAFSFAGPVTYPNARKPIAAARAIPDDLLLAETDAPDQAPAAHRGGR
                     SEPAYVAAVIAGLAAARGVSPREMAELTGANARRVFGDFG"
     gene            22402..23259
                     /locus_tag="VFV99_17750"
     CDS             22402..23259
                     /locus_tag="VFV99_17750"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX5061218.1"
                     /translation="MTHRRFDRAARLLGDDGIARLARSTITVFGVGGVGSFAAEALVR
                     SGVGRLILVDYDRICVTNVNRQIHAMKGTLGKTKVEVMAERLRLINPDATIEARAEFY
                     GPETSARLLVPEPDVVIDAIDNVAAKMHLIATCVRDRLRIVSAMGAAARLDPTQVRVA
                     DLSETRVDPFARDLRRNLRRKHGFDCSQRTGVWAVFSDEPPQPPVSLAYDDGAFRCVC
                     PGGMNGVNDCEHKNRVEGSIAFVPSAFGMTMASVAVKLLCNLPLPKSCDEPTPAKPAI
                     RPARFAAPS"
     gene            23280..23759
                     /locus_tag="VFV99_17755"
     CDS             23280..23759
                     /locus_tag="VFV99_17755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5061219.1"
                     /translation="MIEVGNKVPSVTIKQATPEGPADVNPAELFTGKNVIMFSLPGAF
                     TPTCSAKHLPGYVKELPQLKAKGVDMIVCLSVNDAWTMKAWAEQHDALGKIVMLADGN
                     GDFSKALGIEQDLGKAQMGLRARRGVLHISDGVVKSIEMEAPGKFEVSSAEACLLKL"
     gene            23800..>24575
                     /locus_tag="VFV99_17760"
     CDS             23800..>24575
                     /locus_tag="VFV99_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5061220.1"
                     /translation="MAPPLFHRLLIANRGEVAVRVARACDALGITPVFAVSEADRDAP
                     YTRGREVVVLGPGRAALSYLDVERVVQAAVQSHCTALHPGWGFLSENPLLATLCAQHG
                     VTFVGPPPHVTAMMGSKTRAKAAMVAAGLDVIPGSNGVLADAADAKRVATATGFPVLF
                     KAENGGGGRGMRIARSLDEVERVYGEAQAEALAAFGGDRVFLEKLIEGGRHVEIQVFA
                     DRYGHAVHLGERDCTVQRNHQKLIEESPSPALTGDQRRVV"
BASE COUNT         4485 a         8142 c         8101 g         3847 t
ORIGIN      
        1 gagctgatcg tcgaagactt agaaaaaggg ggctgacgtc tgacgtctga cgtctgaccc
       61 catttctcgg ggtctgaccg gtctgacccc ttttagacga cgcgtttacg cgcgggcggc
      121 gcgccgtacg gcatcggcac gttgttcttg tcgtcgggcg gtggcggcgg tggttgctcg
      181 acgccagcgt cgccgctcgc ggtttgctcg tggtcctgca cgttcgtgtc ggtcttcggc
      241 ttcttgccac cgcatgcggt ggtcgcgagc gcggcacccg cgaagaccgc ggcgcgcgag
      301 acgcggccga gccgcgtgct cgacggtgca gcgacagtca gctccgcgtt gcagaacggg
      361 cacgcgcgct catcgatcgc gacatgacga cgacactccg tacacggcct caactctgcc
      421 atgcgctcga tgctacacga cgcggcgacg cgcgggcggc gcgccgtacg gcatcttcac
      481 cggcgacggc tcgggctcgg cctggccggt ctttgcgtct ggcggtgtct cgtacgtggt
      541 gcccgtcggc gcgtccctga cgcctgcgtc gggcagctgc tgcgccggcg gaatgttcgt
      601 gcgcttcggc gggccgcacg cagtcgtcgc gagcgccgca ctcgcgaaca ccgccgcacg
      661 cgacgcgcga ccgagcacca cgcgcgtcgg cgttgccgcg atcaactcgg cgctacagaa
      721 cggacacacg cgctcgtcga tcgatacgtg gcgacgacac tccgcacacg gccgcagctc
      781 cgccatgcac gaaggctact acacgatgcg gcggcgagcc ggtggcgcac cgtacggctt
      841 cgcgttctgg atgtggtcga tcttgatgtc ccgcacgtcc tgcacctgct gcttctggac
      901 gtgcttcttc ttcacgactc ccgcatccgg aggcgcgccc gcatcgacga cggcagcatc
      961 gggctcgatc acggcggcat cgaccggcgg cggcaccgct gcgtgttcca cggtacgcgg
     1021 ctcgtcgagc atcttctcga gatcgtcgct gccctgcgcg ggtggcggcg cgggcgcttc
     1081 cttcggcttg tctttgccgc aagcgacgag cgcagcactg aacaccgcgg cgcgtgtcac
     1141 gcgaccgatc acggtgatgt gttgcgcgcg cgcgacgggc aatacgtgcg cgcagaacgg
     1201 acacgcacgc tcgcgcgttg cgacgtgacg cttgcatgcg gggcacggca cgagtgcggt
     1261 cacacgatcc tcttccgcgc cggtggcgca ccgtacggct tcgggatgtt gtgcggcggc
     1321 ggaagcggca ccgcttgttc gtgatgcggt ttcttcttct tcacgacacc ggcatccggc
     1381 ggcagtcctg cgtcgacgat cgctgcatcg ggctcgatca cggcagcatc caccggcggc
     1441 actggtgcgt gctcgacggt cttgccgtcg ttgtcgagca tcttctccag ctcgtcgctg
     1501 ccttgcgcag gtggcggcgg cggcgcttcc ttcggcttgt ctttaccgca cgccgcgagc
     1561 gcggcactga acacggccgc gcgtgtcacg cgaccgatca cggtgacgtg ttgcgcgcgc
     1621 atgcgtggca acgcgcgcgc acagaacgga cacgtgcgtt catgcgtcgc gacgtgacgc
     1681 ttgcatgcgg gacacggcgc gagtgcagtc acacgatcct cctccgcgcc ggcggcgcgc
     1741 catacggttt gcagcacggc gcgcggtcgg gaatcgtgag atcgataaac acgtcggcgg
     1801 tcgcgcccgc atcgctctcg agcgtcaccg gcgtaccttg cggcgtctcg ttcggatttc
     1861 ctgactgaaa catcaccgcg tattgtccgg gcggcaggtt cttgaactcg tactcgccct
     1921 tcgcgttcgt cgtcgcgtac accggcgagc cgtcgggctt ctgcacggtg acggtgacac
     1981 ctggcaatgg cgcgccgccg tcggcgttgt gcacgacacc gcgaatcgtg cccggcggtg
     2041 gcaccgacgg cttcgactcg acctgcttgt gctcgatcgg cgtctcgcgc ggcgtcgtcg
     2101 agccagtcca gcacgcgccg gcgagcgtcg cgcccgcgaa cacggccgca cggctaaacc
     2161 gaccgcgtgg gaccaggagc cccatcgcga cgggcgcgag cgcgtgcccg cagaacgggc
     2221 atcgtgcttc gtcgacggcg acgtgacggc gacatccctc acacggacgc aagcgcatgg
     2281 cgacactgta cacctggggc aaggccgcac gcgcgcacgc accgccgcgc gatcacgcca
     2341 cgcgaaaccc gctaggtccg cgtctcgtcc gtaagttaga tcgcagtcga cgggatcctc
     2401 gtcggtacgc gcacttctcg acgcagcggt atctcatgtg cttggaacca cggccgtggt
     2461 ggacttttgt gaattggctc gtcgcgatca cgacgatctc gaccgcgcat tacgcgcgat
     2521 tctcgacccc aaaactgcgg cacgcgaagc ggcaaatctg ctcgatgtat tccggctcgc
     2581 gctcgccgtt catctcgtcg ccgagacccg ggtgcacagc accttgctcg ctctcgtgcg
     2641 acctcccgcg gctctgcgat cgcaaatcgc gcagctgcga cgcgagcacg acgtgcagca
     2701 ggaaacggcc gagcgcctgg ccgagctcga gccgtgtagc gacgcgtggt actgcggcgc
     2761 gctcgagctg cgtgtgcacg tcctcgatca cgcgaaacgc gaggactatt ttcgcgcggc
     2821 gctcgacgat tacgtaccaa gcgccgtcag ccgtgggctc ggcgccgagt acgcgaccga
     2881 gcgcatgaag ctgctcgcta cgacgtcgcc gctcgcggtc gctcgttaca tcaatgtcgc
     2941 gtagacgagg actacgcgac taaacgacgc ggccgcgagc cggcggcgca ccatacggca
     3001 tcgggatgtt cgacggcggc gggataaaga tctgctgatc gacggtcttc gcgtcgtggt
     3061 cgccgagcgt cacgttctgc cgcggcgcgc gccggcgtgg atgattgtcg taaccgaaca
     3121 cgagcacgta atcgccggcc ggcaggtcga cgaacgcgta gtggccgttc gcgtcggtct
     3181 gcgcattgcg cggctgcatg ctcggttgcg acggcgactg cagctgcacc ggccagttcg
     3241 cgagcggcgc agcgctttgc gaattcttca gcgtgccctg gatcgcgctc gtgccggcat
     3301 ctggtggctt cgcgaacttc tgctggtctt cggttatctg ttgctgttgt tgttgctgct
     3361 cgtgcgacgg tggcggaggc ggcggcggtc cgtactgcgg cggcggattg ctcgtgtagc
     3421 aaccggcgag cgcagcaaac gcggcggcac gcgacagccg gccaccgacg gtaacgcgcg
     3481 ctggcggtgg cgaagcggcg gccgcgccgc agaacgggca cgcgggttcg gccgcgaagt
     3541 gacgacgaca ctgcgagcag gcacgcaacg tcatgtactc agcgtaaccg atcgagccac
     3601 gcgacggtgg catcaacgag cgatgcgtcg gcgcgaccgc taccgtaatc gcgcaagctc
     3661 gcgacccggc cggcatgcca gccgaacaga tgatcgaggc caggaaggtc gatcacgctc
     3721 gtcgtacccg gcgcgagatc ggcgatgcgc gattgatcgt cggggcggac gacccagtcg
     3781 tgctcgccac gcacgacgag gaccggcgcg tcgacctcgc gccatgccgc ctcgagatcg
     3841 agtgcgtgaa gctgctcgtg gtacgcggcc gagcgaccgt tgagctcgcc ggtcttcgcg
     3901 aggttctgga tcgcggcgag ttgcgcggcg atctctgcgc tcgtcgcacc gcgcatgcgc
     3961 aactggcgct cggtgctgtc gagcaaacaa tcgagccacc gcatcaccgg cgtaccgtag
     4021 acgatcacgc ctgcaacgtt gcgcgcgagt tgcgacgcga tgatgccgcc gacgctgtga
     4081 ccgaacacga cgagcggaac gttgcgctga gcggcagtct cgcgcgcgag ctcgagcgcg
     4141 gcccgcgcgt cggcgagctc ggtgtgaaag tcggtctcgg cacacggcgg tccttcgctg
     4201 tctccgacgc cacgcttgtc gaaacgcagg gcgtcgtagc cagcctgcgc ccagccggcg
     4261 acgagcgcgc ccagtggcgc atcgggatcg agcgcgaagt cgatcgactc gcacgcgatg
     4321 ccttggatca cgagcacgag cgcacgcggc gtctgcgcgc acgtcaggat cgtccgcagc
     4381 cgcgcatcgc cgacgtcgag cgcgccgtag cgcgcgtcgg gttcgagcgg aaccgcgacg
     4441 acgtcggtac gtgtcacgtg atgcacaccg tcgcgcagca cgatcagctc gacggtggcg
     4501 gcgtgacccg cgatgcgcaa cgcagcggcg agcgtgcgca gatcgtgcac gatgtgaccg
     4561 tcgatcgaga ccagcacatc gcctgcacga aggtcggcgc gcgccgccat cgtgccttcg
     4621 atcacgccag cgattcgcac gccttcgggg ctgaatgcct ccctgtcgcc gggcagctcg
     4681 gcgccgaggt atgcgcggcg tggaagcggg gtggtcaaca tgagtgatgt aggcagactg
     4741 tcacaggaat tgtgcagcca tgggacagcg gcatccagcc tcagcctacg ttacgatggg
     4801 ggcgtgggaa gcagcgaaac gcttcgctcg gtcatcgatg ctggccgcag cagtaaacgc
     4861 ctcgatgagc tgagcagcgc acgcgtgatc ggaaagatcg cgcagcagat tcacgccgca
     4921 caacagaagg cgggcgcggg aaaagctgtc ggtccgatca cgcctgcgaa tatcaagctc
     4981 gccgcgaacg gcgaaaccaa gctcgagctg ggcgccagcg ccggctccct cggctactcg
     5041 tcccccgaac agacgatggg tggcttcggc gatcgccgca gtgacgtgtt ctcgctcggc
     5101 acggtgctgt gggaagcgct cacgtaccag cgcctgttcg acgcgatgaa cgacgcggcc
     5161 gtccgcatgg cggtgcaaga gcgtgagatc tcgccgccgt cggaagtgaa cgcgaacatt
     5221 cccgccgagc tgtccgcgat ctgcatgcgc gcgctcaatc gcaactcgac tgatcgctac
     5281 cagagcctca aggcgatggc ggtcgagatc gaggaattcc tcgaggaagc cggttacgcc
     5341 gacgacgatt cgaagatcgc ggcctacctc gcgacgatga acgatgcgcc gaaggcggtg
     5401 aagatcgaga tggcgccgct cgcgcgcccg gcgagctcga cgcaacctcc gccctcgacc
     5461 ctcaccccgc cgtcgaccac gaagccgccc gtcgagaaat cggcgagcgg cacgcagccg
     5521 ccggcgaacg tgatgaagcc acccgcagca ccgagcatgc tcggcgaggc tccgaagtcc
     5581 acgccggcga aacccgcggg tcacgcactc gcagccgcgc tcgcgaacgc cggcgcgacg
     5641 cccgcgacgt cgaatggctc gacgaccacg gcagcagcga gcacgaccgg caccagcctc
     5701 ggcttgccac cgatcaatcc atccacgacc gccgtcggcg tgccgaaggc cgacgattgg
     5761 caggactcgt cgaagaaagc tgacgcgccg ctatcaccgt tgccgccggt gttgccgtcg
     5821 ggctcgacgc cgctgcgcaa cgatcgctcg acgattgcgg tgtcggtgcc gccgcaagtg
     5881 aaggtggcga ccgtcgaggc gaagcatgcc gaggcgaagc agcccgcgat gatcgtcgac
     5941 gacaagccgg ccgcgcagat cgtcgaagac gtcaaggcga cgatcgtcga ttcgaagccg
     6001 cccgcgatga tcgtcgacga tggcaaggcg acgctcgtcg attcgaagcc gcccgagatc
     6061 gtcgtgccga agctcgtcga cggcaagctg ccgtcggccg tcgagcagaa accggagccg
     6121 gtcaagcaga gcgcggtcga gaccaagccg gtcgcagcgg tgacgccagc cgcggccgag
     6181 acaaagcccg ccgtcgagac gaaacccgcc gacaccaagc gggcagcgtc ggaagccgag
     6241 aagaagccgg aaggccgcaa gcgtgcgccg tcgcaagccg gtggtcgcaa gcgcccgccg
     6301 tcgcagggcg agctgccgca agccgcggcc gcggtgtcac tgcccggcgg tggccgcgac
     6361 tcgaaggacg tcctcgccgg ctggggctgg ggtaccgaca agcacaacgc gatcgcgccc
     6421 gacgattatc cgcacgacga tgactacgac ttccacgagc cgaactcgag gaagatgctc
     6481 gtcaagatca tcggtgccgg catgggtgtc gtcgcgctga tcatcatcgt cgcgttcgcg
     6541 ttcggcggct cgagcgacaa gaagaagacg tcggccgcga agcaagccga cacgaccaag
     6601 ccgctcgtcg agcaacctcc ggcaccaccg cccgaggccg cgcccgtcgc acaacccgcg
     6661 gctggctcgg cggaccccgc gcccggctcg gccgacggtt ctgcagttgc ggcagcgcag
     6721 ccgacggagc cggcgcagac cgaagagcag aaagcagaag cggcgaaggc ggaagctgca
     6781 gccgcggaag ccgcgaaggc cgaagccgcg aaggccgaag ccgatcgcat cgccgccgag
     6841 aaggaacagg cgaagcaggc cgaggttgca aaggctgaag cggcgaagca agaagccgcg
     6901 cggatcgccg ccgagaagga gcaagcgaag caagcagacg ccgcgaagaa agaaaccgcg
     6961 aagcaggaag ccgcgcgaat cgccgccgag aagaaagcgg aagccgcgcg cgtcgccgcc
     7021 gagaaggcgg aggcgaagaa agccgagcag gcgcgcatcg ccgaggcgaa gaaggcgcag
     7081 gccgaagcgc gcaaggcgga agctgcgcgc atcgcagccg agaaggccga agcgaagaag
     7141 acgcaagcag ccgctgcgaa gaccacgccg aaggcgcggc ccgagaagaa gcaaccgaag
     7201 gccgaaacga aggtcgccaa agccgagccg aagaccgaga cgaaggtcga cggcgacacc
     7261 gcgtatcgcc agggcctgca gctgttcgca cgtggcgata ccagtggcgc gctcgcgtcg
     7321 ctgcgcacgg cactcgccgc gaatccgaac cacgcgccga cctggcgcgg cctcggcctc
     7381 gtgttcgaga agatgggcga gaaggaccag gcgcgcgtcg cgtacaagcg ctacctgcag
     7441 ctcgcaccag gcgccggtga cgccgaacag atcaaaggcc gactggagcg gctccagcca
     7501 tgaagcgcgt attcgtgatc gccctgctct gcctcgcggt cggcaggtac cacgctgcga
     7561 gcgctggccc gcgtcaggta ctggtgctga agagcgaagg caccgcggac gcgtcgagtc
     7621 gcacgacgat cgacacgcac gtgctgcgca tcgcgaagaa catcgacggc aacgtcgtcg
     7681 ccggtgacat cacgctcacc gaagcagcgg cgctcgtcgg ctgtagttcg agcgaagccg
     7741 cgtgcaagga tcaagtgctc gacacgctcg gcgtcgacga ggtcgtcgcg tcgacggtca
     7801 ctgcgacacc gaccggcctc aacgtgacgg tgcgacgcat cgcgaagggc acgccgccga
     7861 aggccgcaca gacaacgatc ccctcgggca agacgcccga cgcgaaactc aacgccgaca
     7921 tcggtccgct gttcgggctc accgcacccg ccgtcgtgga gccgccggtg accgctccgc
     7981 cggtgaccgc tccgccacca ccgccgccac cgacgacgac gccaacgacg acatctccga
     8041 cgaccacacc ggcgacgacc acgccgacga caccaccgcg cccggtcacg gccgacgcga
     8101 gcacggcgcc gatgaacgat gtcaccgccg cgccgaacgg cgtcgtgacg cagcagtccg
     8161 aaggcgtgcc gcgccgccgc tggcagaaga tcggtatggg cgtgggcggt ggcctcgtgc
     8221 tgctcgggtt cgtgatgtgg gcgcaggcag cgagcgtgca aggtcagatc gacgacaagc
     8281 ccgagccggt ctcgccgcgc gacttccagg atctgcgtga cctcgagaag aagggcgacg
     8341 atctcgcgag cggcggaaac ttcttcttcg tcgtcggcgc gctcgtcggc ggcgtcagca
     8401 cgtactacta cgtcaaggct ggtcgccatc gcccgcagac ggcgcgcatc acgccgatgg
     8461 cagtgcccgg cggcgccggc gtcacgctca cgctgggagg cgcgccatga agctcgtcgt
     8521 tgccggcatg ttcgtcgtgg tcgtcgcgtt gatcacggcg tcgtcgacga gctgctcgat
     8581 tcaccatcgc agcgatcagt acgcgtgcac cgtgaacgga gactgcgacg gcaatcggat
     8641 ctgcgacaac ggcttctgca tcgcgcctgg caccgtcgac gcgccgcgca ccatcgatgc
     8701 gccgaagccg cccggcgacg gcagcaacgg ctgcccgtcg cagtgcacga cgtgcaacgt
     8761 cacgcagaag acctgcacca tcaactgcgc gatgggagcg aactgcacga accaggtcgt
     8821 gtgcccggcc ggctacaaat gcgacatcca gtgcaacacc gacaactcgt gccgcaacgg
     8881 cgtcaattgt cagcaggccg cgtcgtgcaa cgtcgactgc atcggtaagc agtcgtgcca
     8941 gaccgtcgtc tgcggtcccg gtccgtgcga cgtcggttgc tcgggtccag cgtcgtgtcg
     9001 cggtgtcgcg tgcggcagct cgtgcgcgtg cgacgtgctg tgcaccggca atcagagctg
     9061 cgccgaaggc attctgtgca cgtcactcgc gtgccgcagc gcgacgggca gcggctgcac
     9121 ctcggtgccg acgttctgcc actcgtgtaa ttagcggtac gtcgagctgt tgaaccacca
     9181 ctcggcttgc aggccgagga agtggacgag cttgtgcgag tgccgcggat cgtcgggcac
     9241 gtattgatcc agcgccgcgt cattgagata cgcggctcgg taaacgagac gaatctgcgg
     9301 ccggtcgtag ctcgacggtc ccatcggcga ccacgtgagc atcggcgcga tctggaacac
     9361 ggccggatcc tcggccttga gcgtgatgtt gttgaggccg cgcggaaagc gcgattgata
     9421 cgagatgtcg ccgccgacga agaaatcggt ggtcggcacg cgcacgagcg ggcgcgcatc
     9481 gaccgcgtac tcccagccgt catcggaatc gacggtgtcc tcgtcggcat cgacgaagcg
     9541 gcgtgacagc acgccgacca tcatgtttgc gaacttcgta tcccagttgc cggagatgcc
     9601 gaacgtgaat tcgttcgcgc ggcccgtctt cagatcggca ccgaacgacg tcgggggcgc
     9661 gagctcgtcg aacgcggcga ggcccttcgc gtagcgcgtg aacagattga ggtgacgacg
     9721 gaagcgactg tcgtcggaga aaccgaacgc gccgagctcg gcgccgatca agaagcccgt
     9781 gtccgacggc aacgcctgga gcgtgccgtc catacgcttg aacgtgcccg atggcagcga
     9841 gtgaaactcg ccgtggacct tcagcttggc gccgataccc gcgcccgtcg gcatcatcac
     9901 gtacgcggcg ctcgcactcg cgaccgtgcg ctggcgattg agctgctcga ccgtcgtcgc
     9961 gccttgcact ggatttgcga cttcgacctc ttggaactgg aacgggttgt tgaggcggtt
    10021 gaagccgatg tgtcccgcga tctcgaccga gtctgcggtc gcagcgccat cggcgtcatg
    10081 ccgaagcatg atgtcgcgct tgtacatgag accgccgccg accgtgttga tgtcgtcgag
    10141 cggccagtag tcgaacaagt agatgtcgtc gccgcgatac atgcgcgagc cgacccacag
    10201 tgttaggtgg tcgccgactt ggccgtcgag gaacatgttg cgcagtgcgg gctgcgcgtc
    10261 gaactgacct gtgtcgtgga aaagggttcc tgcgaacgcg agcgtgacga tcggccgcac
    10321 gatgatgtag ctgttcgcgc cgcccgaccc agcctgacgc tcgaagccgt acgagaactc
    10381 gagctcgagg tagctgtcct cgacgatgcg tgggccatgc gcgacgatcg ataccttctg
    10441 cggcttgccg ccgcgcagat cactgcccgc gagcacacgg ccatacgagc cgaagcggaa
    10501 gccgcggtcc ttcgcgtgat agttatccag gtcgaggtcc tcttcttccg ccttttcggc
    10561 ggtcggcgac acttcgggct gggtcaccgt ccgcacctgc ggaacctcga tcggaactgg
    10621 cttcgagctc tgcggctcga ccggtttttc gtccgccgaa cctttcggcg actccttcgg
    10681 cggatctttg ggcgtttcgg ctggcggctc accggccgcg ggttgagccc acgcggggct
    10741 cgcaccgaac gcgagcgcta acgcgagacg cgaaaaccgc atggccgcgt cagccgagca
    10801 cgaaacgtgc ccgcaaaatg gaactgcatc tcgtcgaata tgccgcaact cgcgacggga
    10861 tctgaggttt cgcgttgccg atcgcaacga gcacaccgcc ctcttttgca ccaactatcg
    10921 attgtcctgg acacgtccgc tggcatgcga cttgatgcgg tttcatccga tgcgcttagc
    10981 agcagccgcc ctgatgttcg ttctggtcgg ctgcccgtca tctcaggatc ccaacctgac
    11041 gaacggtgac gccggcacag gagacggtgg cggcgatgcc ggaccgagct gcccggtcgt
    11101 gccgagctgc acgacaacga tccagtacaa gggcgcagga actgcagtgt cgctgcgcgg
    11161 cgacttcgcg agcgacggtt ggacgacggg catcgcgatg acgaagaacg gtgacacctg
    11221 ggaagcgacc gttccggtgc gcgacaacca ggtcatcgtc tacaagttcg tggtcgacgg
    11281 gaactggatc gcggatccgg aaaacccgcg caagagcccc gatggctacg gcgcctataa
    11341 ctcggtcgtg cgcgctgatt gcgatcagtg tccgccgcgc gccgccgtcg attggcgcga
    11401 cgcgatcatg tacttcgtca tgatcgatcg gttcgccgat ggtgacatgg cgaacaacgc
    11461 accggtcgct ggcgtcgagc tgccgggcca gtaccagggc ggcgactttg tcggcctcac
    11521 gaagaagatc aacgagggct acttcggtca gctcggcatc aacacgctgt gggtgacgtc
    11581 gccgctcgac aacgcggaca acgcgaatcc cggctcggac ggccacagct actccggcta
    11641 tcacggctac tggccgcgcg atctggatag cgcggagagc aagctcggca ccgaggccga
    11701 gctcaaggcg atggtcgacg ccgcgcacgc gcacggcatc caggtgctca tcgactacgt
    11761 gatgaaccac gtgcacacgc agtcgccgat ttacgcgcaa catccgaact ggttctggcc
    11821 gaacgacaac ggcttcggcg gcaactgcgt gtgcggcgcc ggctgtagct gggacaccga
    11881 ccgcttgaag tgctggttcg atccgttcct gcccgacttc gatttccgca atcccgacgc
    11941 gcgcaagtac tccgtcgaca acgcagtcgc gtgggcgaaa cggatcggca tcgacggctt
    12001 ccggctcgac gcggtcaagc acatcgagac gtcgtggctg accgacgtgc gcgcgcggct
    12061 caacgccgag gtcgcgttcg accagaagtt ctacatggtc ggcgaaacgt tcgacggcga
    12121 tcgcggcatc atcaagtcct acgtcaaccc gagcacgatg ctcgacggtc agttcgactt
    12181 cccactgcgc ggccaggtgc tcgcgacact gctccgtcgc gacggccaga tgagcgacct
    12241 cgccggcttc ctcgacgcga acgacggcta ctacggccag ggctcggtga tgtcgacgtt
    12301 cctcggcaat cacgacgtgc cgcgcgcgat cgagctcgca ctcgacacgc cgatgttcgg
    12361 cgcgtgggac ggcggcaaga atctcgcgtg gagcggtcag ccgcagctgc cgcaatcgaa
    12421 gaatccattc gagcggctcg cggtcgcgta cacgctgctg ttcacgatgc cgggcattcc
    12481 gatgctgtac tacggcgacg aattcggcat gccgggcgcg ggcgatccgg acaaccgccg
    12541 cttcatgcag tggagcgatt acaccgcgaa ccagacctgg ctgcgcgatc gtctcgcggc
    12601 actcgcgaag atgcgcggcg agcacatcgc gacccggcgc ggcacgcggc agacgctcgg
    12661 cgtcagcggc gatgcgctcg tctacaagat gacctcatcg ggcgataccg tgttcgtcgc
    12721 gctcaatcgc agcgacaacg cacagaccgc gccgaacctc cctgcgggca actacgtcga
    12781 tctgatctcg ggcgctgccg ttaccgcgcc gctgacgttg caaccgcgca gcgccgtggt
    12841 gctgtcgcag cagtaacgtc gtcacgtcgc tacatacctg tgatgttcga ctcgttcggc
    12901 gaacgtgcgc acgcggacac gactccgtcg cgagtgcttc atgtagatcg ggccgagcac
    12961 agctgcgagc acgccgccgg tgccgtcgag gatcagatcc cacatcgtgt cgagaagcgg
    13021 aggcatcgtc ggtgacccct gtgcgtacgt accgaacagc tggtcgctag cgaactcgca
    13081 aatctcccac agcgcaccga gcccgagcgt gatcaagaag atgaagatcg cgtcgagcca
    13141 atgatgcgag cggtccccgc gaatgaagtg ggcgaggtac accgcaagaa acgcgacgaa
    13201 accgatgagc agggaatcga cgaggtgcaa caccttgtcg aacacgtcga cacgcaggta
    13261 caggccgaag agataaccga gtgacatgtg cgcggtgaca aggacgagta gtgcgagctc
    13321 gacctcgatc ggccagatga agtggccggt gcgcgagatg atcgtaggtg tgacgacgag
    13381 cgcgatcgcc acgaagcacg tgaccgcggt cgcccactcc tgctcggcga acaacgcaat
    13441 gcaggtggcg agcagggcga tgcgaagcac ccagacggtg atcagctccc agcgcggaat
    13501 ccctgcgaac actgaatttc gcaggtgcaa gcccaggacc gccgaagtgc gcagtgccgt
    13561 cgtgcgcgcg ccgcaatagg cgaacggctt gcgcgaaagg cggatggcgg cgatgaccta
    13621 cgcgcactta cgctggcgca tcgcttgctg aagctaccta ccggaggcca accatggagg
    13681 tacagcgctg ccgtatctac accgggactg gagagctgca gtcgactcgt actcgtccca
    13741 tggagcggcc tctcgtgccg cgcacaccga cgatcgccga caggatttcg atcagcgcga
    13801 tcatgacgcg cgacatcgtg tgtgcgcggc ccgagctttc tattccggct ctcaagagct
    13861 tgctcgtcca gaatcatatt ggctgtgtgc cgatcgtcga cgatcgcggg cggcccaagg
    13921 gaatcgtcac gaagctcgac ctggtcgagg ccaagcagcc agccctcacg gcggccgacg
    13981 tgatgatgcc gctcgcgatc accctcaaca gtcacgccac catcgcgcat gctgccgcaa
    14041 tgatggcgat cgaggacttc caccatctga tggtagtagg caacgatcac atcctcgtcg
    14101 gcgtggtctc gacgatggac atcgtgcgct ggctcgcgaa gaacgacggt tttactgggg
    14161 cctgacgtgt gctaggtcag ctggatgcgg ctttggtttg ccctgctgct cgcgctcgcc
    14221 aactgtcatc cgtcgccgtc aaaaccggct cctggtgagg tgcctccgct cccgcccgca
    14281 tcgggcactg cggtcggcta tctcgtcgat gccgctggtg agctccagct gcgcgacgat
    14341 caggtgacga agctcaaaga catcgacacc agcctcgccg ccgagaacgg ccagatcgac
    14401 gcgcagctgc gccagatcga gaagccggtg ccggcggaag aggtgtcacc gcaggaccag
    14461 aaggccggcg tgaagccgca gcgctacaac aacgcgcccg gcgcgagcac gatcacgacc
    14521 accgactcgc agaagctccg caagatgcgg gacgacaacg atcgtgacgc gctcatcaag
    14581 gcgttcaagg tgctcgatgc cgagcagctc acgaaggcga agcaaattct cgcagaccgt
    14641 ggcatcgcga ttcctggcga agcaaagcag gagcagacgc acgaccccga ggacggcaag
    14701 ccgctcccgg gtatggaacc gtagcggttg cggcagcgtg cggtttttgc gctagattcc
    14761 gcgccgtgcg tacctcgaag ctcctcccgc tgatgttcat cgccgcgtgc ggcggtccca
    14821 agaagcagcc ggccccaccg gcacccgcgc ccgagccaac ggccggcgcg cctgccgtag
    14881 caccggtgcc aacgccggcg ccaccgcccg gcccgaacga cgccgcgctc gtcgccgagg
    14941 cgaccgaatt cgcgaagcat gccgatgaag agctgcgcaa gctctacgtc gattcgtcgg
    15001 tcgccgactg ggcaaaccag accgacatca cgcccgagca cgaggcggca tcggcgaagg
    15061 catccgaggc gctcgcgaac ggcctcacga agctgatcaa ggagtcgcgc aagttcgagc
    15121 gcgtcctgcc aaagctcgat cccgatacac gccgccagct gtggctgctg aagatcgccg
    15181 gccagcccgc gcccgatgat ccggcgcagg ccgaacagct cgcgaagatc gcggccgaga
    15241 tgacgtcgat ctacggcaag ggcaaggtct gcgatgcgag caagccgaag gccggcgcgc
    15301 cgaagcaagc gcagctcgac gacctcgccg cgaagctcaa agccgagcag gatccgaaga
    15361 agcaagacgc gctgaagaag gatctcgctg cgaagcagaa gaaattcgac caagcgtact
    15421 gcaaggatct cgacgcgctg tcgaaggtgt tgcagaagag ccgtaagccc gacgagctgc
    15481 tcgccgcatg gaaaggctgg aacgacaacg tcggcaaggc gattcgcccg ctgttcacgc
    15541 gctacgtcga gatcgcgaat gccggcgcgc gtggcgtcgg cttcaaggac gtcgccgaga
    15601 tgtggaagtc gggctacgac atgccgcccg atcaattcga gaaggaagtc gatcggctgt
    15661 ggaaccaggt gcgccctctc tacgtgcagt tgcactgcta cacgcgccgc ggcctcaaca
    15721 agctctacgg cgacaacgtc gtctcgaaga ccggcccgat tccgagccac ctcaccggca
    15781 acatgtgggc acaggcgtgg ggctacctct acaaggagct ccagccgtac ccgggcgtcg
    15841 cgccgatcga cgtcacgccc acgctcgaga agaagttcga ctacacgaag atggtgaaga
    15901 tggccgaggg cttctacatg tccctcggta tgaacccgct accgaagacg ttctgggagc
    15961 ggtcgatgtt ccagaagccg aagggcaagg atgtcgtctg tcacgcgagc gcgtgggatg
    16021 tgacgttgaa taacgacctg cgcatcaaga tgtgtatcaa caagaaccag gaagacctgg
    16081 tcacgatcca tcacgagctc ggtcacaact actactacaa caactactac acgctgcccg
    16141 cgctctatca gaacggcgcg aacgacggct tccatgaagc gatcggcgac acgatcgcgc
    16201 tgtcgatgac gccggagtac atgaagcaga agggcctgct cacgaaggtc gagaagaacg
    16261 agaaggcgac gatcaatcag cagatggatg tcgcgctcga gaagatcgcg ttcttgccat
    16321 tcggcctgct cgtcgacaag tggcgctggg atgtgttcag cggcaaggtc aaacccgacg
    16381 agtacaacaa gcactggtgg gacctgaagc tgaagtacca gggcgtcgcg ccgccgatcg
    16441 cgcgcaccga ggccgagttc gatcccggcg cgaagtacca catcccggcg aacacgccgt
    16501 acatgcggta cttcctcgcg gcggtgctgc agttccagtt ccaccgcgcg ctgtgccaga
    16561 aggcgggctt caagggcccg ctcaacgaat gctcgatcta cggcaacaag gaagcgggcg
    16621 aggcattcaa gaagatgctc gcgatgggtg cgagcaagcc gtggcaggac gcgctgttcg
    16681 agctcacggg tcagcgcgag atggacgcga ccgcgatcct cgagtacttc gcgccactgc
    16741 agaagtggct cgaagagcag aacaagggcc agcagtgcgg ctggtagtta cggcgtcggt
    16801 acaagcggcg gcgtaactgg tgccggtgcg ggcggggttt cgcccggcgg ctgcaccggc
    16861 acttctgtcg tttgcatgca gtgaacgatc gcggcgtcgc gcatgaacac ggcgccgtga
    16921 aattcggtgt cgaggccgag cacgacttgc tcgagcgcca tcacggtcgc gcagtcgccg
    16981 ttgcgcgcgg cgatcgcggc ttgcttcgtg agctgccagg cgtcgcggcg tgacggcgac
    17041 acgcgcgggg ccgcggcggc tgctgcctct gccttctgtt gctcgtacag ctcgttgttg
    17101 tgacgacggc acgctctgac gttgatcagg cctgagcgcg cagacagccc caacagccca
    17161 gcggacagcc ccgagacgac agcgcccttt acctggtcgc tgttgtcacc cgagctatcc
    17221 atatcggcca caaacacgaa cgccgtgatc ccgtacacga ccgcgaaggc cgcatcgacg
    17281 aaaggcgatg cctccgatgc attgcagggc tggtctgccg tctctggctt cggcccttcg
    17341 gtgaagaccc acgaacagct gcacaggagc accagcaaca agcgcatcgt ctgcaacgct
    17401 atcgtggccg cgcctcgatt ccaacgactt acggcatcgt gactggcgcc actggtgccg
    17461 gtgcgggcgg ggtttcgccc ggcggctgca ccggcacttc tgtcgtttgc atgcagtgaa
    17521 cgatcgcggc gtcgcgcatg aacacggtgc cgtgaaactc ggtgtcgagg ccgagcacga
    17581 cctgctcgag cgccgccacc gtcgcgcagt cgccgttgcg cgcggcgatc gcggcttgct
    17641 tcgtgagctg ccaggcgtcg cggcgtgcgc gtggaatctc gggtggtgcc gccgcggccg
    17701 cttgttgttg ctcgtacagc tcgttgttgt gacgacgaca cgcgctgacg ttgatcaggc
    17761 ccgagcgcgc agacaatccg aacagccctg cgaccagccc cgtgatgaca acgccggtta
    17821 cctggtcgcc attgtcactc gagctatcca tatcggccac gaacgcgaac gccgcgattc
    17881 cgtacaggac cgcgaacgtc gcatcggcaa ccggcgccgc ctctgatgca ttgcatggct
    17941 tgtctggcgt ctctggcttc ggcccttcag tgaagatgag cgaacagcca cacaggagca
    18001 ccaacaacaa gcgcatcgtt ggaacgctat cgtgaccgcg cctcgattcc tacgaccagc
    18061 gcaaccgaat ggttgcaatg agcggcaatg acgcgacgcg aggctaggcc acctgacacc
    18121 acgaaagtcg gctgacctgg caaggccatt tgccggtcct cgcggtgttg gtctcaagtg
    18181 cgcgttctgc cccgggtctg catcgcggcc cgcctggcat atgcgctgca aaacggtcgg
    18241 gacagcaatc ctcgaccggg agactaccaa tgaagagctt caagaatatc agcttcgctt
    18301 tcgcactcgc gctaggggca gtcactgtcg ctactggctg cgccgccgat tcgcccgagc
    18361 agggtggtgg cggtggtggc ggtggtggtg gtgacggctc tggttccgac gacgctccgc
    18421 gcccgatgga cgcgagcggc aaatactcgg tggtgagcac gttcgacatc gcgacgaacg
    18481 caccgggcaa agtcggtgaa gtcaccaacg cgttcatcgc gatgaccgat ggtgccgcgg
    18541 atcccgccga atggatcatc gagcagatca tcgcgaagac ctcgaacagc acgctcaaga
    18601 gcatcctgac ctcggcgaag ccgttcgtcg cgggttacct caacgaccgc ctcctcgaga
    18661 tcgcgcctga cttcgtcacg acgatcgtgc aggttggaaa cgacttcggc cagatggcga
    18721 agaactttgg cctcaacgag cagctcgatg tctcgggcag cggcatggcg tacacgtcga
    18781 ccgtcagcgc gctcggcgtg cacttcaagg tcgacaacgt cgagagcgac atcatgttcg
    18841 cggatcactc gctcgcggcc gtcaccgccg cgaacgtcgc gatcacgctc gacgcgactg
    18901 gcaagctcga catcggcgag cacaagctgc cgctgtcgta cggcaaggtc ctccgcctcg
    18961 gcctcgacga ggtgatcatc cccgcggttg acccgacggc gacgaacctc aacacgctgc
    19021 tcgcgcacct cgtcgactgc tcggcagtcg gcacggcgat caacgacgcg ctcgtcgacc
    19081 agttcggctt cggcggcggc gccagcacct gggccagcgc gtgcaccgcg ggcctcggcc
    19141 tcggttcgca ggcgatctac accaagatcg attcgatcga ctcgtcggcg ctcgagttcg
    19201 gcctcgtcgg tacggcgaag gctgtcgact cgaacaagga cagcaaggtc gacacgattc
    19261 agaccggcaa gtggaccggc actctgagct acggcaactc gccgtcgccg ctcgcggccg
    19321 cgacgttcaa cggcacgcgt atgtagcttt cgattcggcg atgcgggtgg tgagcccgcg
    19381 cgtcgaggat ggctggccgg ccgggggaat ggtccctcgg ccggtttttt tttcggcacg
    19441 cgaacgccgc aacacgcgtg taactgcttc gaggttcaac tgacctcagc acgactgtta
    19501 acccggccac gcgacttgcc gttgctcacg atcggttgcg tatctgcttg atcgatcggt
    19561 gtgcacaaaa cgagcagcgt ggcacacgcg ctgcaaagtg gttcggccat catgaagaac
    19621 tacagcctct ccttgacctc tctcctcgca ctcggcgtca ccgctgccgg ctgcgctgct
    19681 gatgccccga atgtcggcga cgacgacgac ggtggcggca gcggctctgg ctcgggctcc
    19741 gagaccgtcc ccctcaccgc cgaaggcaaa tacaagatga cgtcgacgtt cgacgtcgcc
    19801 acgaacatgc ccggcaccgc cggcgccgtg atcaaccaga tcatcgacgc caccgatagc
    19861 gccgacgatc cgacgcactg gatcctcgat cagctcgtag cgcagctgcc gtcgggctcg
    19921 ttcaagaaca ccgtccagca agcgattccg ttcgtctctg gctacctcaa cgatcgcctg
    19981 ctcgacgtcg cgcctgactt cgtcgtcacg atccgcgaca tgggcaacaa gttcggccag
    20041 atcgcgcgca acttcggaac gctcgaaacg ctcgaagtcg gtgcgaacgg ccaggcgacc
    20101 aagacggtca ccggcgtgca cttcgtcgtc gaccagctcg agctcgacta cgcgttcgcc
    20161 gactacggcg tgcagcctgt ctcggtgccg agcgtcgtcg tccagctcga gccgaccggc
    20221 cgcctctcga tcggcgagca caaggtgccg ctcgcgtacg gcaaggtgct ccgcatcgcg
    20281 ctcgacgaga tggtgattcc gatgatcgac ccgtcggccg tcacgctcga agacgtgctc
    20341 aagaacgtcg tcaactgcca cgcggttggc cagtacacct acgacgcgct cggcatcggc
    20401 tcgccgagca cgttcgagtc ggcctgcctc ggcggtctaa aggccggcgc gaacgccatc
    20461 tacaaccaga tgggcaaggt cgacagcgtc gcgctcgagt tcggcctcac cggcatcgcg
    20521 aagggcgtcg acaagaacca cgacggcaag ctcgaccaga tccagaccgg cgcgtggacc
    20581 ggcacgctcg catacggcgg cgctggtgcg cctctcgccg cggcgaagtt cgtcggcgag
    20641 cggatgtaac gctagtacat cgatcgttcg ggctcgggcg cacctcggtg tcgctcgagc
    20701 tctgcgttta cgaagcctcc gatcacgagc gcgatcgtcg acaagtaaaa ccacagcagc
    20761 gtgacgatga cgctgccgaa cgcgccgcgg atcagctcgg acgacgagac atagtcgacg
    20821 tagaccgaga gcccgtacga gacgagcaac aacagacatg tcgcgatcac cgcccccggc
    20881 aggcagatgt gtcgcaacgg cgccggcggc agaccgacga caccgccggc atcggtgtgc
    20941 ttctcgggac cgagcggccg cggcgagggc ccgtagcggt agagcgcgag cagcgtgagg
    21001 aacacgatcg cgagcagcgc gggccagcgc aggagcatcg cgatcgggat ccagttgcgg
    21061 ttgatcgcgc cgaccagcgc gggcagcacg atgagcaccg cgaacatcgt gatgatgccg
    21121 atgagggtcg agagtgccat ggtcacggac agcccgagcc tgacgatcgg cttgcggcgc
    21181 tcgcgcacgc ggtacgcccg gttgagcgcg tcgatcagcg agcgcgccga tgcacgcgac
    21241 gagaagacgg cgagcacgat gctcgtgacg agctggaggc tcagctcgcc gctaccccgc
    21301 gacgcctggc gcttgagctg caggccgatg aagtggacga cttcgaccgg cagcacatgc
    21361 tgcaggtttt cgatctgtgg ctggatcttg gtcgggtcgg cgacgagccc gtagatcgcg
    21421 accgccgcgg ccagcgtcgg cacgatcgcg aggattgcga acagcgcggt gcccgccgcg
    21481 aggatcggca tctcgccaag cagcgccgca tgcgccgcac gcaaccagcg cgcgagaacc
    21541 gtttcactgg tccgcaccca gtgagaccat tggcgcacga gcttcactgc cactccctcg
    21601 atgctaaccg ctccgtgatc gacacgcact gccacctgga cactgctgca ttcgatgccg
    21661 accgcgatgc cgtcctcgcg cgggcagcag ccgccggcat caccggaatc ctcgtacccg
    21721 cgattcgccc ggcgacgtgg caaccgctga tcgacctggc ccgccagcgc tcactcttgc
    21781 gtatcgccct tggcgtccac ccgcagatcg tgccggagct tgccgagggc gaggtgccgg
    21841 caaacttcgc cgatgcattg gcgcgcgcga tcgaggacag cggagcggtc gcggtcggcg
    21901 agtgcgggct cgacggcggc accgccgatc agccgcgcca ggaggagctg tttcgcgcgc
    21961 acatccgcgc cgcgcgcgcg acgaaacgcc cgctcgtcat ccacgtgctg cgtgcacacg
    22021 acgccgcacc gcgcatcctg cgcgaggaac gcgccgacga ggttggtggc gtcatgcaca
    22081 gctactccgg tggcgcgcac ctggtgccga tctatcgcgc gatgaacctg gcgttctcgt
    22141 ttgccggccc ggtcacctac cccaacgcgc gcaagccgat cgccgccgcg cgcgcgattc
    22201 ccgacgacct cttgctcgcc gagaccgacg cgccggacca ggcgccggct gcgcatcgcg
    22261 gtggccgctc cgaacccgcg tatgtcgcgg ctgtgatcgc agggttagcg gcggcgcgcg
    22321 gcgtctcgcc tcgggagatg gccgagctga ccggtgcaaa tgctcgtcgt gtgtttggcg
    22381 atttcggcta gagtgcgccc gatgacgcac cggcggttcg atcgtgcagc gcgacttctc
    22441 ggcgatgacg gcatcgcgcg actcgcgcgt tcgacgatca ccgtgttcgg cgttggcggc
    22501 gtcggctcgt tcgcggccga agcgctcgtg cgtagcggtg tcggccgact gatcctcgtc
    22561 gactacgatc ggatctgcgt caccaacgtc aatcgccaga ttcacgcgat gaagggaacg
    22621 ctcggcaaga cgaaggtcga agtgatggcc gagcgcctgc gcctgatcaa tcccgatgca
    22681 acgatcgaag cgcgtgccga gttttacggt cccgagacct cagcgcgtct gctcgtcccc
    22741 gagcccgacg tggtgatcga cgcgatcgac aacgtcgcgg caaagatgca tctcatcgcg
    22801 acgtgcgtgc gcgaccgcct gcgcatcgtg tcggcgatgg gcgccgccgc ccgcctcgat
    22861 ccgacgcaag tccgcgtcgc cgatctcagc gagacgcgcg tcgatccgtt cgcgcgtgac
    22921 ctgcgccgca acctacgccg caagcatggc ttcgattgct cgcagcgcac cggcgtgtgg
    22981 gccgtgttct ccgacgagcc accgcagccg ccggtatcgc tcgcgtacga cgacggcgcg
    23041 tttcgctgcg tgtgccccgg cggcatgaac ggcgtgaatg actgcgagca caagaatcgc
    23101 gtcgagggct cgatcgcgtt cgtgccgtcg gcgttcggca tgacgatggc gtccgtcgcc
    23161 gtcaagctgt tgtgcaacct gccactgccg aagagctgcg atgaaccgac accagccaag
    23221 cctgcgatcc ggccggcccg cttcgccgct ccgtcctgag gtgtggtaac ccaagcggca
    23281 tgatcgaagt cggtaacaaa gttccctccg tcaccatcaa gcaagcgacg cccgaaggcc
    23341 ccgctgacgt caacccggcc gagctgttca cgggcaagaa cgtgatcatg ttctcgctgc
    23401 ccggcgcgtt cacgccgacg tgctcggcga agcacctgcc cggctacgtc aaggagctgc
    23461 cgcagctcaa ggcaaagggc gtcgacatga tcgtgtgcct gtcggtcaac gacgcgtgga
    23521 cgatgaaggc atgggccgag cagcacgacg cgctcggcaa gatcgtgatg ctcgccgacg
    23581 gcaacggcga cttctcgaag gcgctcggca tcgaacagga cctcggcaag gcgcagatgg
    23641 gtctgcgcgc gcgccgtggc gtgctgcaca tcagcgacgg cgttgtgaag tcgatcgaga
    23701 tggaagcgcc cggcaagttc gaagtgtcga gcgcggaagc ctgcctgctg aagctctaga
    23761 cttcagattt cgggctgcgg acttcgggtt ccagacgata tggcccctcc tctctttcat
    23821 cgcctgttga tcgcgaatcg cggcgaagtc gcggttcgtg tcgcgcgcgc gtgcgatgcg
    23881 ctcggcatca cgccggtctt cgcggtgagc gaagccgatc gcgatgcgcc gtatacgcgc
    23941 ggccgcgaag tcgtcgtgct cggtcctggc cgcgcggcgc tcagttatct cgatgtcgag
    24001 cgcgtcgtgc aggcggccgt gcagtcgcac tgcaccgcgc tgcacccggg ctggggcttt
    24061 ctctccgaga acccgctgct cgcgacgctg tgtgcgcaac acggcgtgac gttcgtcggt
    24121 ccgccgccgc acgtgaccgc gatgatgggc agcaagacgc gcgcgaaggc cgcgatggtt
    24181 gccgccggcc tcgacgtgat tcccggcagc aacggcgtgc tcgctgacgc cgccgatgcc
    24241 aagcgcgtcg ctaccgcgac cggattcccg gtcctgttca aggccgagaa tggtggcggt
    24301 ggtcgcggca tgcgcatcgc gcgctcgctc gacgaggtcg agcgcgttta tggcgaggca
    24361 caggccgagg cgctcgccgc gtttggtggc gatcgcgtgt tcctcgagaa gctgatcgag
    24421 ggcggccgcc acgtcgagat ccaggtgttc gccgatcgct acggccacgc cgtgcacctc
    24481 ggcgagcgcg actgcacggt ccagcgcaat catcagaagt tgatcgagga gagcccgtcg
    24541 cccgcgctca ccggcgatca gcgccgcgtc gtttg
//
LOCUS       DASUFX010000443         7383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1877131, whole genome shotgun sequence.
ACCESSION   DASUFX010000443 DASUFX010000000
VERSION     DASUFX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877131"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="bioB"
                     /locus_tag="VFV99_17765"
     CDS             <1..924
                     /gene="bioB"
                     /locus_tag="VFV99_17765"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5061221.1"
                     /translation="RRAYWGRRVSLHVLENAKLGACPEDCGFCSQSNKHASPGGEAPM
                     KSVEELVDAARKAHAARAKRYCMVTATRGPSQRDLDTICAAAQQIKAEMDIELCASLG
                     ILTEAKAQRLVEAGVDRFNHNLETSERHYEKIVSTHTWRDRVETVQIAKRAGMDTCCG
                     GIVGLGESEDDLLDLAFALRELDVDSVPVNFLDARPGTPLAGYPLVEPSYALRALCMF
                     RFVHPRTDLRVAGGREITLRALQAMALYPANSIFTQGYLTTGGATPHADHQMIRDAGF
                     ELELASGEAVPADPAAVAHLARKPSLPQVRS"
     gene            924..2330
                     /locus_tag="VFV99_17770"
     CDS             924..2330
                     /locus_tag="VFV99_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061222.1"
                     /translation="MKRALVLVALLAACRGSKAPPEQNKTAQGSGSAQGSGSQAKDPW
                     QNVDAGPKVAETPEQRKARAEKALARVDAIQPKLAKLRGLPFEHPVPTAYQTTDDFRA
                     FLHREIAKEMPPEKSAKVSAAWLHIGLLQKPIDLATAFEQTMTTQAAAYYDPAAKKFF
                     MVMVPDSDTLLDTMSAHELTHGLQDQNFDLTKYMAAKPPLDDDAQVARQFIVEGDATF
                     AMFLYMAADATKSDAAQAMMIKVLRGQIEQFAKMDLAAYGEMMKQQASAFENMDADLK
                     KSMDSIGELPPVVVGPLLDSYMKGALVVLTAYDKGGWKAVDDFYKHSPESSEQVLHPT
                     TKLFPKREHPKKVTLPKLDGEVLTDNVMGEILWNIYFSLWLPDQAQQASEGWGGDRYT
                     VVKRADGSLLGYLATIWDTPKDAKEFADAYEASFAKRFPSKDRKHVVKLDGQKVFILD
                     GDDDAKLFEKLIKGTKFS"
     gene            2378..2647
                     /locus_tag="VFV99_17775"
     CDS             2378..2647
                     /locus_tag="VFV99_17775"
                     /inference="COORDINATES: protein motif:HMM:NF042751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6665 family protein"
                     /protein_id="HEX5061223.1"
                     /translation="MSNPLGAIERELEGERASALGEAGRKLEAALAELALGETEDRLD
                     EAAMAAWHYIILRESVRMFDHKAALEVYRVPGKVRARIGVMKKPG"
     gene            2687..3262
                     /locus_tag="VFV99_17780"
     CDS             2687..3262
                     /locus_tag="VFV99_17780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5061224.1"
                     /translation="MRIDRVYTKGGDKGETSLIGGERVSKADPRLDCYGTIDELNAVI
                     GLLTEALASSAAGPHLIPILRRVQNELFNLGCELATPDAERRAKLPRVEPRHIEALEK
                     DIDAVNDDLPMLKSFVLPGGGWASAYSHLARTVCRRAERLVVGLSEAEDIGGELPVQY
                     LNRLSDALFVWGRWCALKDGKDEPLWDSRHT"
     gene            complement(3272..4120)
                     /locus_tag="VFV99_17785"
     CDS             complement(3272..4120)
                     /locus_tag="VFV99_17785"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5061225.1"
                     /translation="MITILGTGLLGSGFARAFKKKGHDVRVWNRTPAKAQPLAADGIT
                     VASDAAEAVRGAERVHILVSDDAAVDTVLAAAAPGLTPNTLVFDHSTTTPGGALARTT
                     SWRERGITFVHAPVFMGPQQALESTGLMLISGDRDIVARVSPLLAPMTGKLIDLGPRV
                     DKAAAFKLLGNLILMAFTAGFTDFLTLAKAFGVSPSEAGTLFENFNPGNSLAARYKRM
                     TERAYENPSWELAMARKDARLMEEEAARANLPLIMVPALAKLMDEKIAQGHGHADWTV
                     VGNDVA"
     gene            complement(4130..4672)
                     /locus_tag="VFV99_17790"
     CDS             complement(4130..4672)
                     /locus_tag="VFV99_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061226.1"
                     /translation="MALLVVNDWILKAQWRDGALHAVAGKLSDIAGLAFAPVVLSAAI
                     GLVLALAARLGARIDPSLTRRRLLACIGATAAGFIAVKLSPTASDAVVGALSTFGRPA
                     TIALDRSDLFCLPALAVAYWIGRDELRRVPLGKPAAIHRLGRPAEPALADTRWAGADT
                     YELAAAIDAWDPPRIDALLG"
     gene            4691..6163
                     /locus_tag="VFV99_17795"
     CDS             4691..6163
                     /locus_tag="VFV99_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061227.1"
                     /translation="MHRLTDGQGGFLCSIDAAQRSPQPLHSYTLPVVSESGKQSESAK
                     QLAEAEKQAKERFEKAMTELREGAYRFSSRARGQSELIIEELIDPEGDTVGSLAEVEG
                     EAGALALAQSLELITFDTWLFGQIGDKDALDLKTEDHWEVWFTYGAWIGETMRRRHGG
                     HWLLMGDDPHTWRIGFSKIFLEIAPFVFSEQLLRMGSGATKKMVTEIERIRQLHEDQR
                     KADNDQSLDRFLAQHYIRLHTVPLGQWMSMDFANLAKMWNEEPVEKLVAELKEKGPRL
                     GPQNAQIVEQVVSALARAKQDQPIAKQTGDRGLFEAIAQIIGLRRATAPVAIDILERV
                     VMPALHVGIPEKFPPLDEDDLANLRKGIELFAFFVETVPHKYQADDEGFLGTIPHEQL
                     TTPYADRNVLEIGKGDWVVVNPAHFIPMLQEFDPQKLLDKYDEFVTYVRSLPDAPNRR
                     DDGRMLAETAIRALADLKACVATAAQNQDALLFRLLPPPG"
     gene            complement(6169..>7383)
                     /locus_tag="VFV99_17800"
     CDS             complement(6169..>7383)
                     /locus_tag="VFV99_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5061228.1"
                     /translation="EITLEKILDEEASLRAIAVLRPDGSLVEEASKPVEPAETKWRDA
                     VVDHQLPNGSSLRLTFRVIDTSSELQTLKTKLDAVKQFQQERSAFGGGLQTAFLVVMA
                     LAALAAIAFGIFAGSFVTRRIEALVTTARRVSEGHHDARVELRGRDEMAELGSAFNTM
                     LDDLEHARGQLEYLQRMGVWQDVARKLAHEIKNPLTPIQLAVQQTVSTYKGDDERFKK
                     LLKDMREIVEEEIASLRRLVDTFRTLGQLPKVEKAPIALADLVEELKLDPAFATKLEL
                     RAPREPVTVRADKLLLKRVLANLVENGLHAGQEAGNDGNVVVEWQAGRDSVQIMVDDH
                     GKGVADENRDKIFEPYVTTKATGTGLGLAIARKIAIEHGGELSLAPGKAPTGGARFVI
                     TLPLRGPEASQA"
BASE COUNT         1274 a         2391 c         2543 g         1175 t
ORIGIN      
        1 cgccgtgcct actggggccg ccgggtctcg ctgcacgtgc tcgagaacgc gaagctcggc
       61 gcatgtcccg aggattgcgg gttttgctcg cagtcgaaca agcacgctag cccgggcggc
      121 gaagcgccga tgaagtcggt cgaggagctc gtcgatgcgg cgcgcaaggc gcatgcggcg
      181 cgggcgaagc gttactgcat ggtcaccgcg acgcgcgggc cgtcgcagcg cgacctcgat
      241 acgatctgtg cggctgcgca gcagatcaaa gccgagatgg acatcgagct gtgcgcgtcc
      301 ctcggcatcc tgaccgaagc gaaggcgcag cggctcgtcg aagccggcgt cgatcgcttc
      361 aaccacaacc tcgagaccag cgagcgtcac tacgagaaga tcgtgtcgac gcatacgtgg
      421 cgcgatcgcg tcgaaaccgt gcagatcgcg aagcgcgccg gcatggatac gtgttgcggc
      481 ggtatcgtcg gcctcggcga gagcgaggac gatttgctcg acctcgcgtt cgcgctgcgc
      541 gagctcgacg tcgactcggt gccggtgaac ttcctcgacg cgcggccggg cacgccgctc
      601 gcgggctacc cgctcgtcga gccgagctac gcgctgcgcg cgctgtgcat gttccgcttc
      661 gtgcatccgc gcaccgacct ccgcgtcgcc ggtggtcgcg agatcacgct gcgcgcactg
      721 caggcgatgg cgctgtatcc ggcgaactcg atcttcacgc agggctacct gacgaccggc
      781 ggcgcgacgc cgcatgccga tcatcagatg atccgcgacg ctggcttcga gctcgagctc
      841 gcatcgggcg aagcggtgcc ggcggatccg gcggcggtcg ctcacctcgc gcgcaagccg
      901 tcgctgccgc aagtgaggtc gtgatgaagc gcgcgctcgt cctcgtcgca ttgctcgcgg
      961 cgtgtcgcgg gtcgaaggcg ccgccggaac agaacaagac cgctcagggg agtggctcgg
     1021 cgcagggcag cggttcgcag gcaaaggatc cgtggcagaa cgtcgacgct ggtccgaagg
     1081 tcgcggagac cccagagcag cgcaaggcgc gcgccgagaa ggcgctcgcg cgcgtcgatg
     1141 cgatccagcc gaagctcgcg aagctgcgcg ggctgccgtt cgagcatccg gtgccgaccg
     1201 cgtaccaaac gaccgacgat ttccgtgcgt tcctgcatcg cgagatcgcg aaggagatgc
     1261 cgccggagaa atcggcgaag gtctcggccg cgtggctaca catcggcctg ttgcagaagc
     1321 cgatcgatct tgcgactgcg ttcgagcaga cgatgacgac gcaggcagcc gcctactacg
     1381 accccgccgc gaagaagttc ttcatggtga tggtgcccga cagcgacacg ctgctcgaca
     1441 cgatgtcggc gcacgagctg acgcacggcc tgcaggacca gaacttcgat ctgacgaagt
     1501 acatggccgc gaagccgccg ctcgacgacg acgcacaggt cgcgcgccag ttcatcgtcg
     1561 aaggcgatgc gacgttcgcg atgttcttgt atatggccgc cgacgcgacg aagtccgacg
     1621 ccgcgcaggc catgatgatc aaggtgctgc gcggtcaaat cgaacagttc gcgaagatgg
     1681 atctcgccgc gtacggcgag atgatgaaac agcaagcatc ggcgttcgag aacatggacg
     1741 ccgatctcaa gaagtcgatg gattcgatcg gtgagctgcc gccggtcgtc gtcggtccgc
     1801 tgctcgactc gtacatgaag ggcgcgctcg tcgtgctgac cgcgtacgac aagggcggct
     1861 ggaaggccgt cgacgacttc tacaagcact cgccggaatc cagcgagcag gtgctgcatc
     1921 cgacgacgaa gctgttcccc aagcgcgagc acccgaagaa ggtgacgctg cccaagctcg
     1981 atggcgaagt gctcaccgac aacgtgatgg gcgagatcct gtggaacatc tacttctcgc
     2041 tgtggctgcc cgatcaggcg cagcaggcgt cggaaggctg gggcggcgat cgctacacgg
     2101 tggtcaagcg cgccgacggt tcgctgctcg gttatctcgc gacgatctgg gacacgccga
     2161 aggacgcgaa ggaattcgcg gacgcgtacg aggcgagctt cgcgaagcgg ttcccgagca
     2221 aggaccgcaa gcacgtggtg aagctcgacg gccagaaggt gttcatcctc gacggcgatg
     2281 acgacgccaa gctgttcgag aagctgatca aaggcacgaa gttctcgtaa gcagtttgcg
     2341 cgagacgatc cgcctcggtc gtggcatctg aaggagcgtg agcaacccgc tcggtgcaat
     2401 cgaacgcgag ctcgaagggg aacgcgcgag tgcgctcggc gaagccggcc gcaagctcga
     2461 agccgcgctc gcggagctcg cactcggcga gaccgaggac cgcctcgacg aagctgccat
     2521 ggccgcgtgg cactacatca tcctccgcga gagcgtgcgg atgttcgatc acaaggcggc
     2581 gctcgaggtg tatcgcgtgc cgggcaaggt gcgcgcacgc atcggcgtga tgaagaagcc
     2641 gggctgagcc tccttgctcg cgatgggccg tggtaggaga acggccatgc ggatcgatcg
     2701 cgtctacacc aagggcggcg ataagggcga aacctctctg atcggcggcg agcgcgtgtc
     2761 caaggcggat ccgcggctcg actgttacgg cacgatcgac gagctcaacg cggtgatcgg
     2821 cttgctcacc gaggcgctcg cttcgtccgc cgccggcccg cacctgatcc cgatcctgcg
     2881 ccgcgtgcag aacgagctgt tcaaccttgg ctgcgagctc gcgacgcccg atgccgagcg
     2941 ccgcgcgaag ctgccgcgcg tcgagccgcg tcacatcgag gcactcgaga aggacatcga
     3001 cgcggtcaac gacgacctcc cgatgctgaa gtcgttcgtg ctaccgggcg gcggatgggc
     3061 ctcggcatac agccacctcg cacgcacagt ctgccgccgc gcggagcgac tcgtcgtcgg
     3121 gctttcggaa gccgaagata tcggtggcga gctgccggtg cagtacttga accggttgtc
     3181 ggatgcgttg ttcgtgtggg gacggtggtg cgcgctcaag gacggtaaag acgagccgct
     3241 gtgggattcg cgccacacat gagctgagcg gtcacgcgac gtcgttgccg acgacggtcc
     3301 agtcggcatg gccgtggccc tgcgcgatct tttcgtccat cagcttcgcg agtgccggca
     3361 ccatgatcag cggcaggttc gcgcgcgccg cttcctcctc catcaagcga gcgtccttgc
     3421 gcgccatcgc gagctcccac gacgggttct cgtacgcgcg ttcggtcatt cgcttgtagc
     3481 gcgccgcgag cgagttacct ggattgaagt tctcgaacaa cgtgcccgct tcgctgggcg
     3541 agacgccaaa cgccttcgcg agcgtgagga agtcggtgaa gccggcggtg aacgccatca
     3601 ggatcaggtt gccgagcagc ttgaacgcgg cggccttgtc gacgcgcggg ccgagatcga
     3661 tgagcttgcc ggtcatcggc gcgagcaacg gcgatacgcg cgcgacgatg tcgcgatcgc
     3721 cggagatcaa catgaggccc gtcgactcga gcgcctgctg cgggcccatg aagaccggcg
     3781 cgtggacgaa cgtgatgccg cgctcgcgcc agctcgtcgt gcgcgcgagt gcaccgcccg
     3841 gcgtggtcgt cgaatgatcg aagacgagcg tgttcggcgt gaggccgggt gccgctgccg
     3901 cgagcacagt atcgaccgcc gcatcgtcgg agacgaggat gtggacgcgc tcggcgccgc
     3961 gcacggcctc tgcggcatcg ctcgcgactg tgatgccatc ggcggcgagt ggttgcgcct
     4021 tcgcgggcgt acgattccat acgcggacgt cgtggccctt cttcttgaac gcgcgcgcaa
     4081 aacctgagcc gagcagaccc gtaccgagga tcgtgatcat gggcgcagcc tagcccagca
     4141 gcgcatcgat gcgcggcggg tcccatgcat cgattgcggc cgcgagctcg tacgtatccg
     4201 ctcccgccca ccgcgtgtcc gcgagggcgg gttcggcggg ccgaccgagt cggtgaatcg
     4261 cagccggctt gccgagtggc acgcggcgga gctcgtcgcg gccgatccag tacgcgactg
     4321 cgagcgcggg aagacagaac aagtctgatc ggtcgagcgc gatcgtggcg ggccggccga
     4381 acgtcgacag cgcgcccacg acggcatccg aggcggtcgg cgatagcttg actgcgatga
     4441 agcccgcagc cgtcgcgccg atgcaggcga gcaggcgacg gcgcgtcagc gacggatcga
     4501 tgcgggcacc gagccgggcg gcgagcgcga gaacgaggcc gatcgcggcc gagagcacga
     4561 ccggtgcgaa cgcgagtccg gcgatatccg agagcttgcc ggcgaccgcg tggagggcgc
     4621 cgtcccgcca ctgcgccttg aggatccagt cgttgacgac gagcagggcc acggctgcga
     4681 gcgtgacggg gtgcatcgcc tcaccgacgg gcaaggcggg tttctgtgct cgatcgacgc
     4741 cgcgcagcgc tcgccgcagc cgctccactc gtatactctg ccggtcgtgt cggagtccgg
     4801 caagcagtcc gaaagcgcca agcaactcgc cgaggcggag aagcaggcga aagaacgttt
     4861 cgagaaggcg atgaccgagc tgcgcgaagg cgcgtatcgg ttttcatcgc gcgcccgcgg
     4921 tcagtccgag ctgatcatcg aggagctgat cgaccccgag ggtgacaccg tcgggtcgct
     4981 cgccgaggtc gagggcgaag ccggtgccct ggcgctcgcc cagtcgctcg agctgatcac
     5041 gttcgatacg tggctgttcg gtcagatcgg cgacaaggac gcgctcgatc tcaagaccga
     5101 ggaccactgg gaagtgtggt tcacgtacgg cgcgtggatc ggcgagacga tgcgccgccg
     5161 ccacggtggc cactggctgc tgatgggcga cgacccgcat acgtggcgca tcgggttctc
     5221 gaagatcttc ctcgagatcg cgccgttcgt gttctccgag cagctgcttc gcatgggctc
     5281 gggcgcgacg aagaagatgg tcaccgagat cgagcgcatc cggcagctcc acgaggacca
     5341 gcgcaaggcc gacaacgacc agtcgctcga tcgcttcctc gcccagcact acatccggct
     5401 gcacaccgtg ccgctcggcc agtggatgtc gatggacttc gcgaacctcg cgaagatgtg
     5461 gaacgaggag cccgtcgaga agctcgtcgc cgagctcaag gaaaagggcc cgcggctcgg
     5521 cccgcagaac gcacagatcg tcgagcaggt cgtttcggcg ctcgcgcgcg cgaagcagga
     5581 tcagccgatc gcgaagcaga ccggcgatcg cggtctgttc gaggcgatcg cgcagatcat
     5641 cggcctacgc cgcgcgacgg cgccggtcgc gatcgacatc ctcgagcgcg tcgtgatgcc
     5701 ggcgctgcac gtcggcattc ccgagaagtt cccgccgctc gacgaggacg acctcgcaaa
     5761 cctacgcaag ggcatcgagc tgttcgcgtt cttcgtcgag accgtgccgc acaagtacca
     5821 ggccgacgac gaaggcttcc tcggcacgat cccgcacgag cagctgacga cgccgtacgc
     5881 ggatcgcaac gtgctcgaga tcggcaaggg cgactgggtc gtcgtgaacc ctgcgcactt
     5941 catcccgatg ctgcaggagt tcgatccgca gaagctgctc gacaagtacg acgagttcgt
     6001 gacgtacgtg cgctcgctgc ccgacgcgcc gaaccgccgc gacgacggcc gcatgctcgc
     6061 cgagaccgcg attcgcgcac tcgccgatct caaggcctgc gtcgcgactg ccgcgcagaa
     6121 ccaagacgcg ctgctgttcc gcctgctgcc gccgccgggc taggtgagtc aagcctgtga
     6181 cgcttcgggg ccgcgcagcg gtagtgtgat cacgaagcgc gcgccgccgg tcggtgcttt
     6241 gccgggcgcg agcgagagct cgccgccgtg ctcgatcgcg atcttgcgcg cgatcgcgag
     6301 gccgaggccg gtgccggtcg ccttcgtcgt gacatagggc tcgaagatct tgtcgcggtt
     6361 ctcgtcggcg acgcccttgc cgtgatcgtc gaccatgatc tgcacgctgt cgcgacccgc
     6421 ctgccactcg acgacgacgt tcccgtcgtt gccggcttcc tgtcccgcgt gcaggccgtt
     6481 ctcgacgagg ttcgcgagca cgcgcttgag cagcagcttg tcggcgcgca cggtgaccgg
     6541 ctcgcgcggc gcccgcagct cgagcttcgt cgcaaacgcc ggatcgagct tgagctcttc
     6601 gacgaggtcg gcgagcgcga tcggcgcctt ttcgaccttc ggcagctggc cgagcgtgcg
     6661 gaacgtgtcg acaaggcgcc ggagactcgc gatctcttcc tcgacgatct cgcgcatgtc
     6721 cttgagcagt ttcttgaacc gctcgtcgtc gcctttgtag gtcgagaccg tctgctgcac
     6781 cgcgagctgg atcggggtga gcggattctt gatctcgtgc gcgagcttgc gcgcgacgtc
     6841 ctgccacacg cccatgcgct gcaggtactc gagctgaccg cgcgcgtgct cgaggtcgtc
     6901 gagcatcgtg ttgaacgcac tgccgagctc cgccatctcg tcgcggccac gcagctcgac
     6961 gcgcgcgtcg tgatgacctt cggagacgcg gcgtgcggtc gtgacgagcg cctcgatgcg
     7021 gcgggtgacg aacgagccgg cgaagatgcc gaacgcgatc gcggcaagtg cggcgagcgc
     7081 catgacgacg agaaacgcgg tctgcaggcc gccaccgaac gcggatcgct cctgctggaa
     7141 ctgcttgacc gcatcgagct ttgtcttgag cgtttgcagc tcgctcgacg tgtcgatcac
     7201 gcggaacgtg aggcgcagac tgctgccatt cggtagctga tgatcgacga ccgcgtcgcg
     7261 ccacttcgtc tcggcgggtt cgaccggctt gctcgcctcc tcgaccagcg agccgtcggg
     7321 gcgcagcacg gcgatcgcac gcaggctcgc ttcctcgtcg aggatcttct cgagcgtgat
     7381 ctc
//
LOCUS       DASUFX010000444         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1601582, whole genome shotgun sequence.
ACCESSION   DASUFX010000444 DASUFX010000000
VERSION     DASUFX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601582"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1319
                     /locus_tag="VFV99_17805"
     CDS             225..1319
                     /locus_tag="VFV99_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061229.1"
                     /translation="MKQLLVAAALLCACGPGFKGAEDYRNPRIHDFAGDLSQASQKRD
                     VGTIRAMLRDSVTVGGLWFEDVSCTKQFAFPSEVKGPRLDELARCLTTLQLAVSARND
                     QLPDVLLFTYGPGLELEARIIETQDGPWLAWIGYVARRDLQDALPTVSASALEALRIE
                     GDSQAPLAGPGAFDELTVLKAAYAWMKVCIDANGVVTGAHVREASSPHAARTFGAAIQ
                     TWKFRPFMLGSQPAPVCSMVRMFYPTDKTPDRETLPLPVPEGPGDVVNVPSVMLGKRT
                     AGRINLSPDDIDKVRIQKSRVRKLIAALHYCLDVNGHVSRVRLIRSSGLPHYDDKLVK
                     AAEGWAFAPFLDEGKPVVVCSTTHFVYTQK"
     gene            complement(1379..2863)
                     /locus_tag="VFV99_17810"
     CDS             complement(1379..2863)
                     /locus_tag="VFV99_17810"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5061230.1"
                     /translation="MSSGAGPGLSTKVARGVAWAAGAQAIIAIADIVSVMLVMALWVP
                     VADWGIAALAFPFYTMLDTAADLGVTSSLIQRDDHTPERVSTVFWFNMLISSGLFLVL
                     LVLGPLYGHIVDHPIVGWLLVAYGGKLVFQNVYAIPFALLRKELRFNEIAIARVLAHL
                     AESVARCVFAWLGFTIWCFTLAALTRAFVFGVIMQIRHPFLPRFLFRPREVTQYIKFG
                     LRTAASQVLYQLYTNLDYPIVGYYFGAWANGIYAGAYFFVLEPVKTIANVVIDVAFPT
                     FARLRDDPPMLVDQFIKFTRLNLIAVLPFIVLIALVLPEFLRVFYVGDKWTLNDVPYV
                     VNAAHILCLVGLLRALGFLGPPLLDGIGHPERTLRYMVIATFVVPGMFVVAANVLGDR
                     INFLSVAVGWAVGYPIAFAALCYLVVKAIDLPIGQYLRAAWGIVGCCLGGYVAGLVVR
                     HLLAHESDKMRMVIVAATSFVVTGFLLAFWQKITPRSIGRSLKG"
     gene            2921..>3101
                     /locus_tag="VFV99_17815"
     CDS             2921..>3101
                     /locus_tag="VFV99_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061231.1"
                     /translation="MSERQVQVTPEHYDFERYDDAERWMSYWHQLRAVLSVRPKTVLE
                     IGPGSGVFRNYLRAKG"
BASE COUNT          615 a          964 c         1036 g          486 t
ORIGIN      
        1 tacaagaccg gctcgaacga gacctgccgc gtgaccctgc cgcagtcgac gacgatcggc
       61 gtgatggtga acggctactc gacgagctcg acgttcaagc tcgtcggcaa gaagctctaa
      121 gccactcaga ctgtcttacc ggcgcggccc aagggagcat ggctccacgg gccgcgtcgg
      181 cgttttcggc gcatactcgt cgagaatcgg cgatagtgga gaggatgaag caacttttgg
      241 ttgcggccgc gttgctctgt gcgtgcggcc ctgggttcaa gggcgcagag gactatcgca
      301 atccgcgcat tcacgacttc gccggcgatc tctcgcaggc atcgcagaag cgcgacgtcg
      361 gaacgatccg cgcgatgctg cgggattcgg tgacagtcgg cggcctctgg ttcgaagacg
      421 tcagctgcac gaagcaattc gcgtttccga gtgaggtcaa aggcccgcgt ctcgacgagc
      481 tcgcgcgttg tctgaccacg ctgcagctcg cggtgagcgc gcgcaacgat cagctgcccg
      541 acgtgctgtt gttcacctac ggccccggtc tcgagctcga agcgcgcatc atcgaaacgc
      601 aggatggacc gtggctcgcg tggatcggtt acgtcgcgcg gcgcgacctg caggacgcac
      661 tgccaaccgt ctcggcgtcg gcgctcgaag cgctgcggat cgagggagac tcgcaggcgc
      721 cgcttgccgg tccgggcgcg ttcgacgagc tcaccgtcct caaggcggca tacgcgtgga
      781 tgaaggtctg catcgacgcg aacggggtcg tgaccggcgc gcacgttcgc gaggcaagct
      841 cgccgcacgc tgcgcgtacg ttcggtgcgg cgatccagac gtggaagttt cgcccgttca
      901 tgctcggcag tcagccggcg ccggtttgct cgatggtgcg catgttctac ccgaccgaca
      961 aaacgcccga tcgcgagact ctgccattgc cggtcccaga aggccccggc gatgttgtca
     1021 acgtgccgtc ggtcatgctc ggcaaacgca ctgcggggcg catcaatctc tcgcccgatg
     1081 acatcgataa agtgcgcatc cagaagtcgc gggtgcgaaa gctgatcgcg gcgctgcact
     1141 actgcctcga cgtcaatggc cacgtctcgc gcgttcgact cattcgctcg agtggccttc
     1201 ctcactatga cgacaagctc gtcaaggccg ccgaaggctg ggcgttcgct ccgtttctcg
     1261 acgagggcaa gccggtcgtc gtgtgctcga cgacgcactt cgtctacacg cagaagtagc
     1321 cctacgtacc tggggctttg cgacgagcac gaacctgcac gttcaagctc gacaaacgtt
     1381 agcctttgag cgagcggccg atcgagcgtg gcgtgatctt ctgccagaac gcgagcaaga
     1441 agccggtcac gacgaacgac gtcgcggcga cgatcaccat gcgcatcttg tcgctctcgt
     1501 gcgcgagcag atggcggacg acgaggccgg caacatagcc gccgaggcag caaccgacga
     1561 tgccccacgc cgcgcggagg tactggccga tcggtagatc gatcgctttg acgacgaggt
     1621 aacagagcgc ggcgaacgca atcggatagc cgacggccca gccgaccgcg accgatagaa
     1681 agttgatgcg gtcgccgagc acgttcgccg cgacgacgaa catgcctggc acgacgaacg
     1741 tcgcgatcac catgtagcgg agcgtgcgct cgggatggcc gatgccatcg aggagcggcg
     1801 ggccgagaaa gccgagagcg cgcagcaagc cgacgaggca aaggatgtgc gccgcattga
     1861 cgacgtacgg cacgtcgttg agcgtccatt tgtcgccgac gtagaacacg cgcaggaact
     1921 cgggcagcac gagcgcgatc agcacgatga acggcagcac cgcgatcagg ttgagccgcg
     1981 tgaacttgat gaactgatcg acgagcatgg gcggatcgtc gcgtaggcgc gcaaacgtcg
     2041 ggaacgcgac atcgatgacg acgttcgcga tcgtcttgac cggctcgagg acgaagaagt
     2101 aggcgccggc gtagatgccg ttcgcccacg cgccgaagta gtagccgacg atcggatagt
     2161 cgaggttcgt gtagagctgg tagaggacct ggctcgcggc ggtgcgcagg ccgaacttga
     2221 tgtactgcgt gacctcgcgc ggcctgaaca agaagcgcgg caggaacgga tggcggatct
     2281 gcatgatcac gccgaacacg aacgcgcgag tcagcgcggc gagcgtgaag caccagatcg
     2341 tgaagccgag ccacgcgaac acgcagcgcg cgacggattc ggcgaggtgc gcgagcaccc
     2401 gcgcgatcgc gatctcgttg aagcggagct ccttgcgcag cagcgcgaac ggaatcgcgt
     2461 agacgttctg aaagacgagc ttgcccccgt acgccacgag caaccagccg acgatcgggt
     2521 gatcgacgat gtggccatac agtgggccga gcacgagcag gaccaggaac agcccgctcg
     2581 agatcagcat gttgaaccag aacaccgtcg agacgcgctc gggcgtgtga tcgtcacgct
     2641 ggatcaacga cgacgtcacg ccgaggtcag cggcggtatc gagcatcgtg tagaacggga
     2701 acgcgagcgc cgcgatgccc caatcggcga cgggcaccca cagcgccatc acgagcatca
     2761 ccgagacgat gtcggcgatc gcaatgatcg cttgtgcgcc ggcggcccac gcgactccgc
     2821 gtgcaacctt cgttgagagg ccagggcctg cgcccgacga caccagaccg ctgataccac
     2881 gtggcgcgcg cgggcgcttg ccatgctagc gtcacgccgc ttgagcgaac gccaggtcca
     2941 ggtcacgccg gagcactacg acttcgagcg ttatgacgac gccgaacgct ggatgtcgta
     3001 ctggcaccag ctccgcgcgg tgctgtcggt gcggccgaag accgtgctcg agatcgggcc
     3061 gggttccgga gttttccgga attacctgcg ggcgaagggc a
//
LOCUS       DASUFX010000445         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_71324,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000445 DASUFX010000000
VERSION     DASUFX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71324"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFV99_17820"
     CDS             complement(<1..1151)
                     /locus_tag="VFV99_17820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061232.1"
                     /translation="MACPSTNLMLDYVERRLDPTERDEVEEHADACSACRELIAELAR
                     TTSFERPPSAPGDDPKRIARYRIESRLGSGGMGSVYVAHDPELDRRVALKLVHPELAR
                     CDAVGRLLREGRTLARLTHPNIVTVYDAGSDGDRVYIAMELVEGNTLTAWLDSAPRTW
                     RAITSMFVAIGRGLAAAHRAGVVHRDVKPDNVLVTSDGQPKVVDFGLAGRAPSHDGVL
                     PDPAGRITQDGAVLGTPAFMSPEQWDGGDVGPATDQYSMCSALLAALGNRARPAWLDR
                     AIARGTAQDPTERFASMSALVHALDPDRRTRRTRRLVIASATLALLASIASVALIRSP
                     APHRLEASCRAAADARAALWTPLDQLRVRMRIYATGVPWAHQLWLRTDR"
     gene            1163..1855
                     /locus_tag="VFV99_17825"
     CDS             1163..1855
                     /locus_tag="VFV99_17825"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5061233.1"
                     /translation="MATESADLHTEDLTWARALAGGDRDALDRYESELVPLIDAQLKR
                     RGFTADQIAEVQQTLRARFFVGDAEGPAITGYAGRGRLRSWVLVAALREAVRQRQRFA
                     PELSTDDDGMFDLAERAEVFAQEGTHKDLYRNVFRDAFRKVLGELVARDRNLLRLHVI
                     DELTIDEIGTLHGVHRATAARWLDRVREHVALGVRREVARELGTDMFEASELMQWVKS
                     RIELSLSGLASR"
     gene            1897..>3233
                     /locus_tag="VFV99_17830"
     CDS             1897..>3233
                     /locus_tag="VFV99_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061234.1"
                     /translation="MTKPFLLLSCIHAACVVGIGDAPDPPEPPDGPACSDPRYDDGVC
                     NTNLACDAPDIDCFRTFADDAAATLWYVEREARKAQAEDHPPRALLPPSDPRFVQMRA
                     LLDTAWQAFAKARPVGKLVEYRPALVVIEDGEANAFVSAESATQRQVFAVMVQTGLQA
                     APLPEDARLGVLLHELEHIVGTHGIPGVDEDLRRHYVAAGSEPIGRQQADDPIVHDAI
                     ERWLEMADDVGPYSASELGAFPYRGTLWDLLDMLVDGGMRNDPVHCQNAAQLISSLRS
                     ALASSRDALGGQLTLDLSQMPVRVRDSLVALRDECMPGVTTSFVESLARLFGTTPADI
                     TQSMTDHDLQLVTGKHVVDALATLATDRRTRMRTLEHSVPQSRPWSLARYFSTEEDAD
                     DVAVMVLRAAGRDAESYSAAAVSLVAKDGSAAEARCKSLLAAGTVPPYGVDLT"
BASE COUNT          534 a         1079 c         1094 g          526 t
ORIGIN      
        1 ttgcgatcgg tgcgcaacca cagctggtgc gcccatggaa cgcccgtcgc gtagatgcgc
       61 atgcgcacgc ggagttgatc gagcggcgtc catagcgcgg cccgcgcgtc ggcagctgcg
      121 cggcacgatg cctcgagccg atggggtgcc ggcgagcgaa tcagcgcgac gctcgcgatg
      181 ctcgcgagta gtgcgagcgt tgcgctcgcg atcacgagac gccgcgtgcg ccgtgtgcgt
      241 cgatcaggat cgagcgcatg cacgagcgca ctcatcgatg caaagcgttc cgtcggatcc
      301 tgtgcggtgc cgcgagcgat cgcgcggtcg agccaggccg gccgtgcacg gttgccaagt
      361 gcggcgagca gtgcggagca catgctgtat tggtcggtcg ccgggccaac gtcgccgccg
      421 tcccattgct cgggcgacat gaacgcgggc gtgccgagca ctgcgccgtc ctgcgtgatg
      481 cgtcccgccg gatcgggaag cacgccatcg tgcgacggtg cacgacccgc gagcccgaag
      541 tcgacgacct tcggctggcc gtcgctggtc acgagcacgt tgtcgggctt gacgtcgcgg
      601 tgcacgacac cggcgcgatg cgcagctgcc aggccgcggc cgatcgcgac gaacatcgat
      661 gtgatcgcac gccacgtgcg cggcgcgcta tcgagccacg ctgtcagtgt gttgccctcg
      721 accagctcca tcgcgatgta cacgcgatcg ccatcgctgc cggcgtcgta gacggtgacg
      781 atgttggggt gcgtgagccg cgcgagcgtg cggccctcgc gtaacaagcg gccgaccgca
      841 tcgcaacgcg cgagctcggg atggactagc ttgagcgcga cgcgccgatc gagctcggga
      901 tcgtgcgcga cgtagacact gcccataccg cccgagccaa gtcgcgattc gatccggtaa
      961 cgcgcgatgc gtttcggatc gtcgcctggc gccgacggcg ggcgctcgaa cgacgtggtg
     1021 cgtgcgagct ccgcgatcag ctctcggcat gcgctacagg catcggcatg ctcttcgacc
     1081 tcgtcgcgct cggtaggatc gaggcgccgc tcgacgtaat cgagcatcag gttcgtcgag
     1141 gggcatgcca tactcgatca ggatggcgac ggagtcggcg gacctccata ccgaggatct
     1201 gacatgggct cgcgcattgg ccggcggcga ccgggacgcg ttagatcgct acgagtcgga
     1261 gctagtgccg ttgatcgacg cacagctgaa gcgccgcggg tttacggccg atcagatcgc
     1321 ggaagtgcaa cagacattgc gtgcgcgctt cttcgtcggc gacgccgaag gcccggcgat
     1381 cactggttat gccgggcgtg gccggctgcg cagctgggtg ctcgtcgcgg cgctccgcga
     1441 agcggtgcgt caacgccaac gcttcgcgcc cgagctatcg acagacgacg acgggatgtt
     1501 cgatctcgcc gagcgcgccg aggtgtttgc ccaggagggc acgcacaagg atctgtatcg
     1561 caacgtgttt cgcgatgcgt tccgcaaggt attgggcgag ctggtcgcgc gcgatcggaa
     1621 cctgttgcga ctgcacgtga tcgacgagct gaccatcgac gagatcggca cgttgcatgg
     1681 cgtgcaccgg gcgaccgcag cgcgctggct ggatcgcgtc cgcgaacatg tcgcgctagg
     1741 cgtgcgccga gaggtcgcgc gcgagctcgg cactgacatg ttcgaggcat cggagctgat
     1801 gcagtgggtc aagagcagga tcgagctgtc gctgagcgga cttgccagtc gctgaagaaa
     1861 tcgtgcgact gatcccgcgc gctcgttctt acgagcatga ccaagccatt cctgcttctg
     1921 tcgtgcatcc atgctgcctg tgtcgtcgga attggagacg cgccagaccc acccgagccg
     1981 cccgacggac cggcgtgcag cgacccgcgt tacgacgacg gcgtttgcaa cacgaacctc
     2041 gcctgcgacg cgcccgacat cgactgcttc cgcacgttcg ccgacgacgc ggccgcaaca
     2101 ctctggtacg tcgaacgcga agcccgcaag gcacaggcag aagaccaccc gccgcgcgca
     2161 cttctcccgc cgtccgatcc gcgcttcgtg cagatgcgcg cgctgctcga caccgcgtgg
     2221 caagcgttcg cgaaggcgcg accggtcggc aagctcgtcg agtatcgacc cgcgctcgtc
     2281 gtgatcgagg acggcgaggc gaacgcattc gtgtcggccg aatcggcaac gcagcgtcag
     2341 gtcttcgcgg tgatggtgca aaccgggctg caagcggcgc cgctgcccga agacgcgcgc
     2401 ctcggcgtgc tgcttcacga gctagagcac atcgtcggca cccacggcat tccgggcgtc
     2461 gacgaggatc tgcgcaggca ctacgtcgcc gccggcagtg aaccgatcgg ccgccagcaa
     2521 gccgacgatc cgatcgtcca cgacgcgatc gaacgctggc tcgagatggc cgatgacgtc
     2581 ggcccctact ccgcctccga gctcggcgcg ttcccgtacc gcgggaccct gtgggacctg
     2641 ctcgacatgc tcgtcgatgg cggcatgcgg aacgatccgg tccattgtca aaacgccgcc
     2701 cagctgatca gctcgttgcg cagtgcgctc gcgagctcgc gtgacgcgct cggtggacag
     2761 ctcacgcttg atctctctca gatgccggtg cgagtgcgcg attcgctcgt cgcgctccgc
     2821 gacgagtgca tgccgggcgt gaccacctcg tttgtcgaat cgctcgcgcg gctgttcggc
     2881 acgacgcccg ccgacattac ccaatcgatg accgaccacg atctgcagct cgtcaccggc
     2941 aagcacgtcg tcgatgcact cgccacgctc gcgaccgacc gccgcaccag gatgcgcacg
     3001 ctcgagcact cagttcctca aagtcgcccg tggtcgttgg cgcgttactt ctccaccgaa
     3061 gaagacgccg acgatgtcgc ggtgatggta ctgcgcgcgg ccgggcgcga cgcggagagc
     3121 tactcggccg cggcggtgtc gcttgtcgcg aaggacggaa gtgccgccga agcacgctgc
     3181 aagtctctac tcgccgccgg cacggtgcct ccgtatggcg tcgacctgac caa
//
LOCUS       DASUFX010000446         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_350225,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000446 DASUFX010000000
VERSION     DASUFX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350225"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..860)
                     /locus_tag="VFV99_17835"
     CDS             complement(180..860)
                     /locus_tag="VFV99_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061235.1"
                     /translation="MKAAFVIVAAAVLGCKGKDCPPTQNAPVPETSAKPVATKCDTDP
                     VFTHVDRDQLAALCVVAELPAPTVPSDTGALLLADGSRVPWVTVWMDEQFAAGDRPMM
                     TAKAWTAKARAYMERVARSWDRPPQPSKPDGREWRNVLCGGDEALRVVISGVFFEKGA
                     AHAVAVRGKQVLDEQMPANTPHDQVFRKAFELTTKVLGQPATIATHKLAVDPLSTVDT
                     EDWVKAAK"
     gene            complement(888..2138)
                     /locus_tag="VFV99_17840"
     CDS             complement(888..2138)
                     /locus_tag="VFV99_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061236.1"
                     /translation="MRAALLSLCTIALLGCQAGVSGDGGEDACEGPLGKPMSQSEVSA
                     MTTCCQAEAGQAHCLDDGRVPSELQAFVAKCDSGGYCIPDSFLATGASEPPQQCMAFG
                     GSGVCLSRCIPQVAENAGLLRKETCTGADELCVPCISPLDNMPTHACDLLELATCVGE
                     GGGSGGGGQVTSCDDPNTCNYEPNCPAVIDPTALTSCGPDAHCLDAALITDPAQSSRL
                     AKCTDQSKLCVPDIFIKTGGKFTAPSCTSVGGAEGRCLSRVLPDVKAQEALLPQDTCA
                     ASERCTPCYDPITGMSTGACNLSCDTGPTKPAVTFAQCCDMRAHCVPSSAIPDAQEDN
                     LDQDSCTDTNLCVPDQILLNQPIPTCSANSLVLGNYTGVCLSDCFSFGIQGIALARGN
                     CQSNYKCAPCTQNGQPTGAPGCPM"
     gene            complement(2237..3310)
                     /gene="murG"
                     /locus_tag="VFV99_17845"
     CDS             complement(2237..3310)
                     /gene="murG"
                     /locus_tag="VFV99_17845"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX5061237.1"
                     /translation="MKLLIAGGGTGGHLFPGVAIAEELRAREPDAAIKFVGTRRGIEA
                     RVLPDLGWDLEFIEVSGLKTVGAAGAVKGLFRLPRAYWQARRIVKQFKPDAVIGVGGY
                     ASGPVVLAAKLRGIPTAICEQNSIPGLTNKMLGKLVRRVFLSFDESKRFFNTKKIVMS
                     GNPVRRDLVQKLLAASPETRAAVNVLVVGGSQGAVAVNELASKALVVLAKESSIAITH
                     QTGEKDLEPTTGRYREAGITADCRAFIRDMAAAYQHADLIIGRAGATTVAELAIAGKP
                     AIFIPYPFAADNHQELNAREMADKGAALMFRQSDLTAEKLVDALRPLLADPSKRAEMG
                     AAMKSLAKPGAAAAVIDWASGHL"
     gene            complement(3307..>4498)
                     /gene="ftsW"
                     /locus_tag="VFV99_17850"
     CDS             complement(3307..>4498)
                     /gene="ftsW"
                     /locus_tag="VFV99_17850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5061238.1"
                     /translation="AVQHPSATLPERAYDLMLLAAVLALLGVGTIEIYSATAAESLTQ
                     FGSSSHYLQRQIMYVAIGGFALWLGSRIDYRKLRGWTYPLLLISIVCLAAALGMPAKN
                     GAKRWIPLGPLTFQPVEIAKLALVTYLAYSLGRKADRVKTFTVGFVPHLVVCGVMMGL
                     LLLQPDLGSSVVLGMTTLGMLFMAGTRVSYILLAVLGAAPVAYHLVVGTPWRMHRFLA
                     YFNPEAFANTEAYQYLQARLAIGSGGLTGAGLGDGHQTLGYMPMAHNDFIMAPIGEEL
                     GWFGIALVLLLFAILVWRGIRAALGARDVFGGYLAFGITLLFGVQALFNVGVVLGIVP
                     NKGITLPLVSYGGSSLVITLFLVGLVLNVGRRPERATSRAATQRDPSARKKRVRVRVM
                     TAAS"
BASE COUNT          758 a         1481 c         1503 g          756 t
ORIGIN      
        1 gcacatgccc atgccgccaa tgccgacgaa gtggatctta gtgtcgtgtt tgcgaaacat
       61 gggccttgtg ggatcaacgc cgaattgggc gagattatgt cgttccgagc ttgataccag
      121 attctccggg gcggcggata cgcggcgaat ctctcagtcg tcgcgcttag ctacgccgct
      181 tacttcgccg ccttcaccca atcctcggta tcgaccgtcg acaacgggtc gaccgcgagc
      241 ttgtgcgtcg cgatcgtcgc gggctggcca agcaccttgg tcgtgagctc gaacgccttg
      301 cggaatacct gatcgtgcgg cgtgttcgcc ggcatctgtt cgtctagcac ttgcttgccg
      361 cggaccgcga ccgcatgcgc ggcgcccttt tcgaagaaca cgccgctgat cacgacgcgc
      421 agcgcttcgt cgccgccgca cagcacgttg cgccactccc tgccgtccgg cttcgacggt
      481 tgcggtggcc gatcccacga cctcgcgacg cgctccatgt acgcgcgcgc cttcgcggtc
      541 cacgccttcg ccgtcatcat cggcctgtcg ccggcggcga attgttcgtc catccacacc
      601 gtgacccacg gcacgcgact gccgtcggcg agcaacaagg caccggtatc cgatggcacc
      661 gtcggggccg gcaactcggc aacgacacac agtgccgcga gctgatcgcg atcgacatgc
      721 gtgaacaccg gatcggtgtc gcacttggtg gcgaccggct tcgcagacgt ctccggcaca
      781 ggcgcgttct gcgttggcgg acagtctttg cctttgcagc cgagcacggc cgcagcgacg
      841 atcacgaatg cagctttcat cgcacgcact atacgcgcga tcgcgtctta catcgggcag
      901 ccaggtgctc cagtcggctg gccgttctgc gtgcacggcg cgcacttgta gttcgactgg
      961 cagttgccgc gcgcgagcgc gatgccctgg atgccgaagc tgaagcagtc cgagaggcac
     1021 acgccggtgt aattgccgag gacgaggctg ttcgcgctgc aggtcgggat cggttggttc
     1081 aggaggatct gatccggcac gcacaggttc gtgtcagtgc agctgtcttg atcgaggttg
     1141 tcttcttgcg cgtcaggaat cgcgctcgaa ggcacgcagt gcgcgcgcat gtcgcagcac
     1201 tgcgcgaacg tcaccgctgg cttcgtcggt cccgtgtcgc acgacaggtt gcacgcgccg
     1261 gtcgacatgc cggtgatcgg gtcgtagcaa ggcgtgcagc gctcgctcgc cgcgcacgtg
     1321 tcttggggca gcagcgcttc ctgtgccttg acgtcgggca gcacgcgcga gaggcagcga
     1381 ccttccgcac caccgaccga cgtacacgac ggcgccgtga atttgccgcc ggtcttgatg
     1441 aagatgtccg gcacgcacag cttgctctga tcggtgcact tcgcgaggcg cgacgactgc
     1501 gcgggatcgg tgatgagcgc ggcgtcgaga cagtgcgcgt cggggccgca tgacgtcagc
     1561 gctgtgggat cgatgacagc cgggcagttc ggttcgtagt tgcacgtgtt cggatcgtcg
     1621 cagctcgtga cctggccgcc gccgccgctg ccgccgccct cgccgacgca cgtcgcgagc
     1681 tcgagcagat cgcacgcgtg ggtgggcatg ttgtcgagtg ggctgatgca cggcacgcac
     1741 agctcgtccg cgccggtgca cgtctccttg cgcaagaggc cggcgttctc ggcaacctgc
     1801 gggatgcagc gcgagaggca cacgcccgat ccaccaaatg ccatgcactg ctgcggcggc
     1861 tccgacgcgc cggtggcgag gaagctgtcg ggaatgcagt agccgcccga gtcgcacttc
     1921 gcgacgaacg cctgcagctc gctcggcacg cggccgtcgt cgaggcagtg cgcctgaccg
     1981 gcttcggctt ggcagcacgt ggtcatcgcg ctgacctccg actgcgacat cggcttgccg
     2041 agcggaccct cgcaggcgtc ctcgccgccg tcgccagaaa cgccggcctg acaacctaac
     2101 agcgcgatcg tgcacaacga gaggagggcg gcccgcatgg tgccgcgacg ggtttcacgt
     2161 tccgcgccag ttcttgacga ccggtcaaca ggcacttccc cgtcgacgcg gggggaagcc
     2221 tcacccgctc tggaacctag aggtgcccgc tcgcccagtc gatcacggcg gcggccgcgc
     2281 cgggcttcgc gagcgacttc attgccgcgc ccatctctgc gcgcttgctc ggatctgcga
     2341 gtagcggacg cagtgcgtcg acgagcttct cggcggtcag gtccgactgc ctgaacatca
     2401 acgcagctcc cttgtcggcc atctcgcgtg cgttgagctc ctggtgattg tcggccgcga
     2461 acgggtacgg gatgaagatg gccggcttac ccgcgatcgc gagctcggca accgtcgttg
     2521 caccggcgcg gccgatgatc aggtccgcgt gctgataggc ggccgccatg tccctgatga
     2581 acgcacggca atcggcggtg atgccggcct cgcgatagcg tccggtcgtc ggctcgagat
     2641 ccttttcgcc ggtctgatgt gtgatcgcga tcgacgattc cttggcgagc acgacgagcg
     2701 ctttcgacgc gagctcgttc accgcgaccg cgccttgcga cccgccgacg acgagtacgt
     2761 tgaccgcggc gcgggtctca ggggaagcgg cgagcagctt ctgcacgaga tcgcggcgca
     2821 ccgggttgcc gctcatcacg atcttcttcg tattgaagaa gcgcttgctc tcgtcgaacg
     2881 agaggaacac gcggcgcacg agcttgccga gcatcttgtt cgtgaggccc gggatcgagt
     2941 tctgctcgca gatcgcggtc gggatgccgc gcagcttcgc ggcgagcacg acaggcccgg
     3001 atgcgtagcc accgacgccg atcacggcat cgggtttgaa ctgcttgacg atgcggcgcg
     3061 cttgccagta cgcgcgcggg agtcggaaca ggcccttcac cgcgcccgct gcaccgacgg
     3121 tcttgagtcc cgaaacctcg atgaattcga gatcccagcc gaggtcgggc agaacacgcg
     3181 cctcgatgcc gcgtcgcgtg ccgacgaact tgatcgcagc atcgggctcg cgcgcgcgca
     3241 gctcctcggc gatcgcgacg ccggggaaca agtggccgcc ggtgccgccg cctgcgatca
     3301 atagtttcat gacgctgcag tcataacgcg aacgcggacc cgtttcttcc gtgcggatgg
     3361 gtcacgttgc gtcgctgcgc gactcgtcgc gcgctccgga cgcctgccga cgttcagcac
     3421 gagcccgacg aggaacaacg tgatcacgag cgagctgccg ccgtagctga cgagcggcag
     3481 cgtgatgccc ttgttcggga cgatgccgag cacgacgccg acgttgaaca gcgcctgcac
     3541 gccgaagagg agcgtgatgc cgaatgcgag gtagccaccg aacacgtcgc gtgcgccgag
     3601 cgccgcgcga atgccgcgcc acacgaggat cgcgaacagc agcagcacga gcgcgatgcc
     3661 gaaccagccc agctcttcgc cgatcggcgc catgatgaag tcgttgtgcg ccatcggcat
     3721 gtaaccgagc gtctgatgac cgtcaccgag acccgcgccg gtgagaccgc ccgagccgat
     3781 cgcgagtcgc gcctgcaggt actgatacgc ctcggtgttc gcgaacgctt cgggattgaa
     3841 gtacgcgagg aagcgatgca tgcgccacgg cgtgccgacg acgaggtgat acgcgaccgg
     3901 cgccgcaccg agcaccgcga gcaggatgta cgagacgcgg gtgcccgcca tgaacagcat
     3961 gccgagcgtc gtcatgccga gcacgaccga cgagccgagg tccggctgca agaggagcag
     4021 gcccatcatc acgccgcaca cgacgaggtg cggaacgaag ccgacggtga atgtcttcac
     4081 gcggtccgct ttgcggccga gcgagtacgc gaggtacgtg acgagtgcga gcttcgcgat
     4141 ctcgacgggt tggaacgtga gcggaccgag cggaatccac cgcttcgcac cgttcttcgc
     4201 aggcatgccg agcgcggcgg cgaggcacac gatcgagatc agcagtagcg gatacgtcca
     4261 gccgcgcagc ttgcgatagt cgatgcgcga gccgagccag agcgcgaagc cgccgatcgc
     4321 gacgtacatg atctggcgct gcaagtaatg cgaggagctg ccgaactgcg tcagcgactc
     4381 ggccgcagtt gccgagtaga tctcgatcgt gccgacgccg agcagcgcga gcaccgcagc
     4441 gagcagcatg agatcgtagg cgcgttccgg aagcgtcgcg cttggatgtt gcaccgcg
//
LOCUS       DASUFX010000447         5519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1045075, whole genome shotgun sequence.
ACCESSION   DASUFX010000447 DASUFX010000000
VERSION     DASUFX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045075"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFV99_17855"
     CDS             complement(<1..693)
                     /locus_tag="VFV99_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061239.1"
                     /translation="MLATGALTGLIAGFAAGLIDSVWSWAPAAQFVPGAAARLRFVLY
                     TGLSLGAALALAGLFAAVGVLVLSRYTRLGDLIRFGWQEHQTRRANDPREAVIGLSFV
                     LTGLPLVGVAIYVAYRVMTPVVVKSHALGLATLSVIAAALVALGVAVVLMFVIARVVE
                     EGLRALAPRVPLIASPWAPIVAIGVIVVAAAAVWAVREWEIARILPLRGPVVIAAAAV
                     LAIPAAGPARRTV"
     gene            767..2422
                     /locus_tag="VFV99_17860"
     CDS             767..2422
                     /locus_tag="VFV99_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061240.1"
                     /translation="MSADRLRWLLLGVAAVGLVVHSFQYNFITDDAYISFVYSRNFAD
                     HGELVFNLGQYVEGYTNFLWTILIGLGMKAGLDADLVAKILGAVFAVVTLYVTCLTVE
                     RALGRKTPWATVPALLLAASSGFACWTSGGLETQLFTMLVIIALDGLVAAEDVVPAGR
                     SESGRLHPGGESALYRTGIALALAAMTRPEGLLVAAVFGFVRIACNLLSKRRFIGMPE
                     IFAAILFLSLWAPWFAWRYWYYGYPFPNTYYVKATGPWANKGMAKEMYQHGAYYIWVW
                     LKQTKLLYALPIAGLGLMAARPRTPRFALGLTCALLAAVYIPYTISVGGDFMGLHRFI
                     MPLFVIAAIAIALGLEWLIGRLPEHMRTYAGPAAVVAVVGAFAASQIQLTKKSLEWGN
                     FKADRGIIDTPAFLIAYTEDRATIGNAMQSCFHPDDFSIVGGAGAQPYFGNMRSIDVF
                     GLVSARVAHEEPRIRARAGHTKFASDTLLAQYDPTFVFSCYSIHPNPFRPSLNCNPSF
                     WLARGYEQVTMWIPGLRQLGEPFTYYTFLAKKARNFQCPGRVN"
     gene            2440..4416
                     /locus_tag="VFV99_17865"
     CDS             2440..4416
                     /locus_tag="VFV99_17865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5061241.1"
                     /translation="MIPAALVVGLFALRACTATPPLDNLRIVAADPVNDPPGTVAREG
                     SLYIARGGPVIISFQSASPGRLVLGTLGSSSYKQLHGVAPKERIVLPAGPIAIRFAGP
                     PVARLLWSPVGRRGDLEYVPASSLSPEPPERAQFGAGAGAAISDGVISLALLLVLVGT
                     LLMLARHRLREVKRETWLAMGAVFIAACVIRWIGLHAFGQTWDEDVNWSSGRNYVTNI
                     LSLDLSPLSWIWNYQHPPVMKYLEGIGAQFADGYGPARALSAIWVALGCAFLVPIGAR
                     LYSFRVGVLAAIIAALLPPLVAHGQIVGHEAPTVLWWSLGILLALTIHDGEPSLRTLR
                     IRLVWLGVVIGIAVASRFVNGLLGVLVLVIVAERAPAKRALRTAIEGAAIMPIVAALT
                     LYAVWPRLWTHPFSSLDESLAKLTQPHSPEPFLGAITSTPGPHYFLIYLLATLPLGIL
                     AGVIAYCVRAARERNRAALVIAAWFIIPMAVIASPVRQDGVRYVMPCVLALAVAGAAG
                     WDFLVTLLESRVRRAFAIATGVITVYLAITLAIVHPYYLDYFGEQVGGAGTVARNHLF
                     ETAWWGEGVGPAVDYVNEHAAKDAKVYRNCIEPAHLAWFREDLWLPMTNDTASADWIV
                     AYSQQSRRCNVPDGFKRVFIVDVQGATLAEVWQR"
     gene            4969..>5519
                     /locus_tag="VFV99_17870"
     CDS             4969..>5519
                     /locus_tag="VFV99_17870"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5061242.1"
                     /translation="MARVRKRHLQQEMFDSRGKPKGRGGKRRGAGRKPKGKRAGAPHK
                     ARPELKRNQPVHVVLRVAEDVGNLRRRYMYKALREATIAVAMREVNDARDGAFRIVHL
                     SIQRNHVHLLAEADHKRALSRGMQSFQISAAKHLNRAVSLKSIKLAGKDRARAMAARR
                     RGTVFPDRFHQEVITTPKQAHHAL"
BASE COUNT          862 a         1936 c         1759 g          962 t
ORIGIN      
        1 gacggtgcgg cgggcagggc cggccgcggg aatcgcgagc accgccgccg ccgcgatgac
       61 gaccggtccg cgcagcggca ggatgcgggc gatttcccat tcgcgtacgg cccacaccgc
      121 agcggccgca acgacgatca cgccgatcgc gacgatcggc gcccatggcg acgcgatcaa
      181 cggcacgcgc ggagcgagcg cgcgcaagcc ctcttcgacg acacgcgcga tgacgaacat
      241 cagcacgacc gcgacgccga gcgcgaccag cgctgccgcg atcaccgaga gcgtcgcgag
      301 gccgagcgcg tggctcttca cgacgaccgg cgtcatcacg cgatacgcca cgtagatcgc
      361 gacgccgacc agcggcagcc cggtgagcac gaacgacagg ccgatcacgg cctcgcgcgg
      421 atcgttcgcg cgtcgcgtct ggtgctcctg ccagccaaac cggatcagat caccgagccg
      481 cgtgtaccgg gacagcacga gcacgcccac cgcggcgaac agccccgcca gcgcgagtgc
      541 ggccccgagt gacaggccgg tgtacagaac gaatcgtaga cgcgcggccg cgcccggcac
      601 gaattgcgcc gccggagccc acgaccacac ggaatcgatg agcccggccg caaatccggc
      661 gatcaggccg gtgagcgcac ccgtagcaag cacgtccccg agtgggcgtc gcgggctggc
      721 ttccatcgat ccccgtcaca ttagcacccg cggtatggtc tccgcgatgt ccgccgatcg
      781 cctgcgctgg ctcctgttgg gagtagccgc cgtcgggctc gtcgtgcatt cgttccagta
      841 caacttcatc acggacgacg cctatatctc gttcgtctat tcacggaact tcgctgacca
      901 cggcgagctc gtgttcaacc tcggccagta cgtcgagggc tatacgaact ttttgtggac
      961 gatcctcatc gggctcggca tgaaggccgg cctcgacgcc gacctcgtcg caaagatcct
     1021 cggtgcggtg ttcgcggtcg tcacgctcta cgtgacctgt ctcaccgtcg agcgtgcgct
     1081 cggccgcaag acgccatggg cgaccgtgcc ggcgctgttg ctcgccgcgt cgtcggggtt
     1141 cgcatgctgg acgtcgggcg gtctcgagac ccagctgttc acgatgctgg tcatcatcgc
     1201 tctcgacggc ctcgtcgccg ccgaggacgt cgttcccgcc ggccggagtg aatccggccg
     1261 gctccatcct gggggtgaat ccgcgctgta ccgcactggc atcgcgctcg cgctcgcggc
     1321 gatgacgcgt cccgagggcc tgctcgtcgc agccgtgttc gggttcgtgc gcatcgcctg
     1381 taacctcctc tcgaagcgcc ggttcatcgg catgcccgag atcttcgccg cgatcctgtt
     1441 cctgtcgctg tgggccccgt ggttcgcgtg gcgctactgg tactacggct atccgttccc
     1501 gaacacctac tacgtcaaag caactggccc gtgggccaat aaaggcatgg cgaaggagat
     1561 gtaccagcac ggcgcctact acatctgggt gtggctcaag cagacgaagc tgctctatgc
     1621 cctaccgatc gccggcctcg gcctgatggc agcgcggccg cgcacgccgc gcttcgcgct
     1681 cggtctcacg tgcgcgctgc tcgccgccgt ctacatcccg tacacgatct ccgtcggcgg
     1741 cgacttcatg gggctgcacc gcttcatcat gccgctgttc gtgatcgccg cgatcgccat
     1801 cgcgctcggg ctcgagtggc tcatcggtcg cctgcccgag cacatgcgca cgtacgcggg
     1861 tcccgccgcg gtggtcgcgg tcgtcggcgc atttgccgcc tcgcagatcc agctcacgaa
     1921 gaagagcctc gagtggggca acttcaaagc cgaccgcggc atcatcgaca cgccggcgtt
     1981 cctcatcgcc tacaccgagg atcgcgcgac gatcggcaac gcgatgcaga gctgttttca
     2041 ccccgacgac ttctcgatcg tcggcggcgc cggcgcgcag ccgtacttcg ggaacatgcg
     2101 ctcgatcgac gtgttcggcc tcgtctccgc ccgggtcgcg cacgaggagc cgcgcattcg
     2161 cgcgcgcgcc ggtcacacga agttcgcctc cgacacgctg ctcgcgcagt acgacccgac
     2221 gttcgtgttc tcctgctatt cgatccaccc caacccgttc cggccctcgc tcaactgcaa
     2281 tccgagcttc tggctcgcgc gtggctacga gcaggtcacg atgtggatcc cgggcctcag
     2341 gcagctcggc gagcccttca cgtactacac gttcctcgcg aagaaagcgc gcaacttcca
     2401 gtgccccggg cgcgtgaact agtcgcgaaa ttctggggag tgattccggc cgcgctcgtc
     2461 gtcgggctgt tcgcgttgcg cgcgtgcacg gcaaccccgc cgctcgacaa cctgcgtatc
     2521 gtcgcggccg acccggtcaa cgatccgccg ggcaccgtcg cgcgcgaagg ctcgctctac
     2581 atcgcgcgcg gtggcccggt catcatctcg tttcagtcgg cgtcgccagg ccgtctcgtg
     2641 ctcggcacgc tcggcagctc gtcctacaag cagctgcacg gcgtcgcgcc aaaggagcgc
     2701 atcgtgttgc cggcggggcc gatcgcgatt cgcttcgcgg gcccgcccgt cgctcgactg
     2761 ctctggagcc cggtcggccg ccggggcgat ctcgagtacg tgccggcatc gtcgctgtca
     2821 cccgagccgc ccgagcgcgc gcagttcggc gcaggcgcgg gtgccgcgat cagcgacggc
     2881 gtgatctcgc tcgcgctgct gctcgtgctc gtcggtacgc tgctgatgct cgcgcgtcat
     2941 cgcctgcgcg aggtcaaacg cgagacctgg ctcgcgatgg gcgcggtgtt catcgccgcg
     3001 tgcgtcatcc gctggatcgg ccttcacgcg ttcggccaga cctgggacga agacgtcaac
     3061 tggagctcgg gccgcaacta cgtcacgaac attctctcgc tcgatctgtc gccgctgtcg
     3121 tggatctgga actaccagca cccgccggtg atgaagtacc tcgaaggcat cggcgcgcaa
     3181 ttcgcagacg gctacggccc ggcgcgcgca ctctccgcga tctgggtcgc gctcggctgc
     3241 gcgttcctcg tcccgatcgg cgcgcggctc tacagcttcc gcgttggcgt cctcgccgcg
     3301 atcatcgccg cgctccttcc gccgctcgtc gcacacggcc agatcgtcgg ccacgaagcg
     3361 ccgacggttc tctggtggtc gctcggcatc ttgctcgcgc tcaccatcca cgacggcgag
     3421 ccgtcgctgc gcacgctgcg catccggctc gtctggctcg gcgtcgtgat cggcatcgcg
     3481 gtcgcgtcgc gattcgtcaa cggcctgctc ggcgtgctcg tcctcgtgat cgtcgccgaa
     3541 cgtgcgcctg cgaagcgcgc gctgcgcacc gcgatcgagg gcgccgcgat catgccgatc
     3601 gttgccgcgc tcacgctcta cgcggtatgg ccgcggctgt ggacccatcc gttctcgtcg
     3661 ctcgacgagt cgctcgccaa gctgacccag ccgcattcgc ccgagccatt cctcggcgcg
     3721 atcacgagca cgccgggccc gcactacttc ctgatctatc tacttgcgac gctgccgctc
     3781 ggcatcctcg ccggcgtcat cgcgtactgc gtgcgtgcag cccgcgaacg caaccgcgcc
     3841 gcgctcgtca tcgccgcatg gttcatcatt ccgatggcgg tcatcgcctc accggttcgt
     3901 caggatgggg tgcgctacgt gatgccgtgc gtcctcgcgc tcgcggtcgc gggcgccgcc
     3961 ggctgggact ttctcgtcac gctcctcgag tcacgcgtgc gtcgcgcatt cgcgatcgcg
     4021 accggcgtca tcacggtcta cctcgcgatc acgctcgcga tcgtccatcc gtactacctc
     4081 gattacttcg gcgagcaggt cggcggtgcg ggtacggtcg cgcgcaatca cttgttcgag
     4141 accgcgtggt ggggcgaagg cgtcggtccc gccgtcgact acgttaacga gcacgcggcg
     4201 aaagacgcga aggtctaccg aaactgcatc gagccagcgc acctcgcgtg gtttcgcgag
     4261 gatctgtggc tgccgatgac gaacgacacc gcgtcggccg actggatcgt cgcgtactcg
     4321 cagcagtcac ggcgctgcaa cgtgcccgac ggcttcaagc gcgtgttcat cgtcgacgtc
     4381 cagggcgcga cgctcgccga agtctggcag cgctgaacgc ggccgggtac gtcgcagcgt
     4441 gccgatgtgc caagaaacga gcgagggtct gcacgcaatg acgttggcta cgcccgatga
     4501 cgtagctgcg gggcctgctg acagcagccc gcttatggtc ggcgtcgtca gcaggttcgg
     4561 gaatcagaac gcgaacacag tgctcgcgcg aacatctagc gcactgcagt tacgagacgt
     4621 ccgggagacg aatgcggtcg gctgtcgtgc agcgtccggg cgtgggcgct ctgcgcggca
     4681 ccaccgcctg ccgagaacac gttcgcaact gcggggcacc accctcccgg gcgggtttct
     4741 tagatcgacg tcgcgacggc gccgcgtaga gcgcgaccgc tccgctgcag cgatcatcgg
     4801 cgcggattct gatcgatgtc gctgtgatct gcgttcactg gcgccgtctc gctttttgca
     4861 ctctgttggg tctgtgagga gccaaaacag tatctaaaat gaccagtgta aaaatatcgg
     4921 cgatttcata ttgacgattt ccagtcgggt tgttatcggt tgtgaaccat ggcgcgtgtc
     4981 cgcaaacgcc acctccaaca ggagatgttc gacagccgcg gcaagccgaa gggccgcggt
     5041 ggcaaacggc gcggcgcggg tcgcaagcca aagggcaaac gtgccggcgc accgcacaag
     5101 gctcggcctg agctgaagcg gaaccagccc gtgcacgtgg tgctacgtgt cgccgaggat
     5161 gttgggaatc tgcgtagacg ctacatgtac aaggcgttgc gtgaggccac gatcgcggtc
     5221 gcgatgcgcg aggtcaacga cgcacgcgac ggtgcatttc gcatcgttca cctcagcatc
     5281 cagcgcaacc acgtgcacct gctcgccgaa gccgatcaca agcgcgctct ctcgcgcggc
     5341 atgcagagct tccagatctc cgctgcgaaa catctcaacc gtgccgtgag tctcaagagc
     5401 atcaagctcg ccggtaaaga ccgtgcgcgt gcgatggcgg ctcgccggcg cggcaccgtg
     5461 tttccggatc gctttcacca ggaggtcatc acgacgccga agcaagcgca tcacgcgct
//
LOCUS       DASUFX010000448        25813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1183613, whole genome shotgun sequence.
ACCESSION   DASUFX010000448 DASUFX010000000
VERSION     DASUFX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25813
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183613"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFV99_17875"
     CDS             complement(<1..866)
                     /locus_tag="VFV99_17875"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5061243.1"
                     /translation="MSFLRKMFSGKVSAEDPRRFLVESMLGAMEADGDVTEEEMATFE
                     GSLANHPLFEGLSGEELGRLTEIAADAIRVAGGGKARLPAIAAGLPSRNQRLAAYAMA
                     AEVCVADRELAESEIDFLDGMQSEFALEESEAKEVFEAARKHSGLLTLEEKSEKVRFL
                     MPAFVKCMALMAAADGEIHHEERLGMRAVLRNIPDMSVLTTAELDEAIEVALERISGK
                     DNKAELALVAKEIAQASDRYWVTVYVMIIALADGKNDWREIEFLASMKKTFGLSDTQM
                     DVAMRTAAQFPA"
     gene            924..1289
                     /locus_tag="VFV99_17880"
     CDS             924..1289
                     /locus_tag="VFV99_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061244.1"
                     /translation="MRGAILLVLLAGCVIEGPDGMDRGPAYTQAEKAWLAEALPVLQA
                     NCVVCHSASDVRGGIGFLDGETAWDIRSNLLASGEVNLDRPETSRLLSKGAHQGPAMT
                     ATEASKLLFWIQAERDERF"
     gene            1296..1589
                     /locus_tag="VFV99_17885"
     CDS             1296..1589
                     /locus_tag="VFV99_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061245.1"
                     /translation="MARTIIGAFIIAFLVLCAVTTYRNVFADDSAVRAMAEKLAHDTG
                     GCTGDCKTTRIEGKRGVFSTAFEFTMQNGAGIGVTCRRPYVAFGDYACTATKH"
     gene            complement(1586..3532)
                     /locus_tag="VFV99_17890"
     CDS             complement(1586..3532)
                     /locus_tag="VFV99_17890"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5061246.1"
                     /translation="MAVRVLGPTSITRAGVAVPLPRSRKVRGLLAFLALSPQPVSRAR
                     LCDLLWDVPNDPRGELRWCLSKLRGLLDDGTRRRVVTADDHVALDLTDCRVDVLELDK
                     AARGGMAEVPHARLAELCQLFSGDLLDGVMIDGNPEFTGWLAAQRNRYRVLHVEVLRA
                     LAACAPGSDEVFRHLEGWLQIAPFDPRAHEVMLEVLVACGRHHDAEQHLATTIRSFEQ
                     EGIDWVPLRDAWRALRETRAARIEVAAVQPKGEATTARRASIAVMPFVDASAADGRVA
                     DGLTEDIIMQLAKLRVLFVIARGSVFSLAERGVGAQEAARILGVDYVVNGSLRRQGTQ
                     FVVKVELSDARDGRILWTDALPGPAGETLAVLDAIVNRIVAAIADEVESAESKRAVLK
                     PPSSLDAWEAYHRGLWHMYKFTGADNRQAEQFFRAALDLDVTFSRAHAGLSFTHFQNA
                     FLGLTPDHDRQIELAFETAGRSLSADDHDPAAHWAMGRALWLRDAQSESVAELERSVE
                     LSPNFALGHYTLGFVHSQLGDPRAAIDATNHSRQLSPFDPLQFAMLASRALAHVRLDE
                     RVEAAEWAVRATSRPNAHAHILAIAAECLALTNRYDEAKRFVGRIRERLPAYSVEDFL
                     RAFRFEPDVQRQFRLGAASIGFDR"
     gene            3637..4575
                     /gene="cysK"
                     /locus_tag="VFV99_17895"
     CDS             3637..4575
                     /gene="cysK"
                     /locus_tag="VFV99_17895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5061247.1"
                     /translation="MIYDDITQTIGHTPVVRINRLAPKHVTMYVKIEAANPGGSVKDR
                     LALGIIEDAERRGQLKPGGTVVEATSGNTGIALALVCASRGYPFVAIMPESFSVERRQ
                     IMRAYGAKVVLTPAAERASGAVRLAEQLSKERGWFLARQFDNEANPAYHRQTTGPEIL
                     SDFAGMRLDYWVTGYGTGGTLTGAGQMIKCARPETKIIACEPAAAALLDGKEWKSHKI
                     QGWTPDFIPSVLDRDVYDEVLAVSDDKAIATARDLGIREGIFCGISAGGTFAGALAVA
                     EKAPAGSVILAMLPDTGERYLSTVLFEGVPTGSDSV"
     gene            complement(4572..5042)
                     /locus_tag="VFV99_17900"
     CDS             complement(4572..5042)
                     /locus_tag="VFV99_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX5061248.1"
                     /translation="MQDVTDPRDPVAIKKAQGQVAELKAMFDASAAARAERQRAKPLY
                     ERLGGRGPITAVVTDIVELHFTEPLTAPLCKGVDKAKLIGHVVDWLCQAGGGKEKYTG
                     RDMVSAHAHLHMTDVHFMAAGDQIVRTLKKYGVPDPEIQEVMCAIIAHHDDVIR"
     gene            5230..6369
                     /locus_tag="VFV99_17905"
     CDS             5230..6369
                     /locus_tag="VFV99_17905"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HEX5061249.1"
                     /translation="MCRWLVAGLLAGCYAPAPPAGAPCVDGVCPSGLVCSPATNTCEK
                     ESVDPTRDGSIDGPNPMIDAAIDAPKDGSTSPFMYRRRITIQAGAALPAGYTIRVQLN
                     NILGTLVSQGKVKGDFSDLRVIGDAPLGERNRIIDGAPAPAAINFSLQQSIAASTTNQ
                     SYYVYYGNPNATAAPANGTQVFTLYDDFNSGIASFWLKNDAPTTTGGKLVLRAAHTDA
                     ITTNAASDGVPIVSAVELSASTTNPTSDPTVQPEGTFYYWWGYQHTGDFSASSPWVIW
                     IARGKGAVHAEHNGPVGCEAGCTGPDLGQNTSLHYYAIERDTNATRFYLDGTLQYTAT
                     VTNNADYSLMVRNYQAAGDVQVDWIRARARVTPEPAVTLGAEEML"
     gene            6398..7840
                     /locus_tag="VFV99_17910"
     CDS             6398..7840
                     /locus_tag="VFV99_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5061250.1"
                     /translation="MTPDTPHGTWIHTDVPPRLDALGWSRWHRRVVIALGITWILDGL
                     EASLVANLAPTLMHPDTLGLSGAEVGFANTAYLIGQVLGALGFGYLTDRLGRKRLVLI
                     TLALYLVSTALSGFAPNFAVFVFFRFFAGAGIGGEYSAINSAIDELVPARIRGQIDLG
                     INGSYWVGVALGAGVTLIVLDPAIVPVSIGWRLAFAFGSLLGLGILVIRRDVPESPRW
                     LLMHGHVAEANKTIARIEAQAHGELVVDTGVRPAPVRFKVTGPVGLRHLIHTLFVRYP
                     RRTVLGLSLMLAQSFLYNSIFFSYGLILQKFHHVDPERVGLYIVPFAIGNFLGPLTLG
                     HLFDRWGRRTMIPATYALSATLLLLTGALFLGGYLTALTQALAWSAVFFVASAAASSA
                     YLTVSELFPLELRGMAIAVFYAFATLVGAGAPTLFGAIADTGEPNQLFAGYALAAGLM
                     YIAAIVARKFGVAAEGRSLEDITAHPEQPD"
     gene            complement(7841..8485)
                     /locus_tag="VFV99_17915"
     CDS             complement(7841..8485)
                     /locus_tag="VFV99_17915"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX5061251.1"
                     /translation="MLWAKFWVTTHRWLAIVLVSLLVVWSVSGLLFHIKPGWDRAYDQ
                     LSVERRDSVLPLQTFAPPQNLGSSITRIELFDTAIGPLYRVRTSSGTELYDAATGKPR
                     TLDATAVRTLIADAIERSQFRAAYGEITNVAIAGDTARVMTSGGPVVELGVHDARASQ
                     RGRDTDRIDWLYRIHYLQWTGNRTIDKWLALAGLALIWAVMIPGVVLFVRRFRR"
     gene            complement(8530..8787)
                     /locus_tag="VFV99_17920"
     CDS             complement(8530..8787)
                     /locus_tag="VFV99_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061252.1"
                     /translation="MSQPNPALTEVPRSQPPEAWLEELIALLDRAAKLAAANGLAGEV
                     FKRVAWNAYLDARPGLRERLEDKQLRSQLRKLRKRGLIGLA"
     gene            complement(8858..9787)
                     /locus_tag="VFV99_17925"
     CDS             complement(8858..9787)
                     /locus_tag="VFV99_17925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061253.1"
                     /translation="MAGCQMCGADHDGGPETCPKSRVGKVIANKYELVSLIGCGGIAA
                     VYEAHHRLLRRDVALKILHPRFAKDEELAKRFVREARETAAIGHAAFVAVHDAGVSED
                     GCAFIEMARLEGRDLWSIREEQGPIDPARMVKIAIEVLHGLEALHKRGVVHRDLKSAN
                     IFIEAGSDRVRLLDLGFAKATDEHQLTDAEQLLGTPLYISPEQCFDPRQVDGRADLFS
                     LGVVMFENMTGSWPYTWSSKRELLKHVMKGQLERHPAVRRAEVPAWLDDIVARSLAFD
                     REKRFANATDMRAALERGGGRAQRPSLLKRLLR"
     gene            complement(9810..11231)
                     /locus_tag="VFV99_17930"
     CDS             complement(9810..11231)
                     /locus_tag="VFV99_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061254.1"
                     /translation="MGKLGVMARCGCAIAIACISARNVAAGTVTFDNGVTGAGFTSVT
                     PDDYGTHGFAIGQQYDDEYYPPGVSNPFSPTHLTGPELFITTAGNHTSSVLLTDYKVW
                     ADFVENPPPTPDGILADPMHTALARSVTTAIAMTGTNQATSAFRLATDAQGGIRIDVA
                     LVQSLTSDAVARSSQLDYVYTFTNNGTVPVSLVLHFMWDSDLFYNGANKDDDDVVGVG
                     PGLCGVYQHDGDPRWSIGLGNGPLSTVPLTYYYGGKDGFVPGSGPAFVPDNAAIGQQY
                     IWLDHGMPAEWQNYVVGPGKNAVGESGSVIGDATIGIEYHFSLAVGASETINARRYYG
                     TTMIPCFVSANCGNGVVDSGELCDGADTPTCNGATCTASACGDGYMNTVAGEQCDSSG
                     VDSETCNGATCLTSACGDGYVNAAAGEDCETGELCDTSTCTFNFSVGGGCAGCGVGGM
                     PDASWLLGGGLLLLRRRRRSTRR"
     gene            11274..12971
                     /locus_tag="VFV99_17935"
     CDS             11274..12971
                     /locus_tag="VFV99_17935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5061255.1"
                     /translation="MLPRGLAALAVVAALAGTARAQEATDQLGIERFRIAIDRAGILD
                     VEWAGVPGHLSWGAGVLVGFAHDPLVVYDRAMSAVDALVDRRLTTTVVGSIALWNRLQ
                     LGLTVDIVGYQSGEDVLATMGALPTSGLGDMRLLAKLLIAGDARYQLALIPALTVPAG
                     EARGYLRESGAAFAPALAASVRSGRVRGALNLGYAIKPRVEAAGLVSDDEAFARLGAG
                     VTLGAPAAPAAELWLATSIATPLTDAAHNQVAIELLAGAARRFTPTLEGFIGGGIGLD
                     NGFGTPDWRALAGVRFEVASGDRDRDGVASEADRCPDEPEDRDGFADSDGCPDPDNDG
                     DGVADARDRCANEVEDNDGFQDDDGCADRDNDGDGVADAQDRCPDKPEDKDGFQDDDG
                     CPDDIARVDGVVVDPDGRPLAGAKISIVQYAKPSAAPIELTAGDDGKFSTQLDGGALD
                     VTASAAEYKTGTTSATVEPGTHADVTVKLVRAVRQGQLRGQVLSFNGKPLAATITVRG
                     KTTTSATTDADGQFTIELPEGPFSVEIASPGFVTQKRNVTIKLDGVTVLNVDLRGGS"
     gene            12968..14767
                     /locus_tag="VFV99_17940"
     CDS             12968..14767
                     /locus_tag="VFV99_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061256.1"
                     /translation="MIRIVVFALALCACKKSPDPVVARLDAITAHVERMPREAAPWQT
                     ARVGDGFVIGSAVRTGAASHAKLTVGKSGKLDVDANAVVYFTRTPGRDRSDLRVETGV
                     AELETGEETFGVGEATLDPNTHVRIAASPQGTSLVVTVGRATLDDTVIAAGQQITIGP
                     AGKQVAAPPDAGITAPAGQIAIAVRDKPARVTAGGTATELAVGEHLVADGATLDVPAG
                     STVEVSRGGARAVTTGPSVLRVGDKEALVAVTAGGIALHAETADAIAIVPGGTVTAKQ
                     GGDVALDVDAKETAIDAQRGETSVQTPRGTQSLAAGQSALLAKSGEITLVPAPPARSV
                     VTITAGESPTLHDPRAPTPLRVAFDQACPSGGTVEVAKDRAFKKIIARSGGTGAANVL
                     VPAGSFNYRVRCAGGRGASGTLRVAKDSGRTPLPKAAARTTVEMDGREYTILYQNLLP
                     ELTLAWKTAPRGAKYTFVVKPGRGADERIASATPTTKLAAGDVREGSYKVWCEADGGR
                     RSEESRIVIDFDNAAQSASIDAVEVSGTTMRIKGTVIESSTVTAHDAAIGLDRHRRFD
                     AELTTREGEDGVGVRIAHPKAGIHYYVMRAGAS"
     gene            14783..16396
                     /locus_tag="VFV99_17945"
     CDS             14783..16396
                     /locus_tag="VFV99_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061257.1"
                     /translation="MLGETLGSYRIVSEIGSGGMGVVYLAEHTLIGRKVAVKLLRADM
                     PSEYVERFFTEAKAAAQLHHPGLVDVFDFGHHKDGRAYIVMEFLEGESLAERLAREPR
                     LPVPLACIITRGVAIALHVAHEKGIIHRDLKPGNIFLVPDTEAPAGIRAKVLDFGIAK
                     LVRDREERSVKTASGAVIGTPRYMSPEQCKNARTVDGRTDIYSLGCILYEMLLGVAPF
                     DYDSWAELVGAHLYEVPPKPTEIDAKVPADVETLVTKMTEKLPADRFQSMQDLAQSLE
                     VMIATHAGEAGRLTPTSLQRAASTPPSGVRKLEGASDPTIPGSSAEMMAVRAPTTGSG
                     SKKSDAITPTLSAEMTPAKSAPRKLPWVAIAIGGVGITALAITAFVVLGKDTKPQPET
                     AYIVVDQRGSAEAVGSAAAKPVEDSPPTVNSPPTVDAGVAEVHETGSAVLPKMPKTTD
                     LDALTRTFGKQSPAITACFKKHPATSEQVSVRIQIDTRGVVKAAEVLPETVGASPLGA
                     CIADVTRKTSFGLQPKPATFRVPLVKNER"
     gene            16434..16763
                     /locus_tag="VFV99_17950"
     CDS             16434..16763
                     /locus_tag="VFV99_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061258.1"
                     /translation="MKSLLFVVTVAACGGPSQQQIVEQPAATAPFPHTEAPPASTSDQ
                     DRSHLNQQFEDMQWTQDAYRQAEGAERAKTPPPKKQAAPPNPKKAPVEQAPMPKKKAP
                     VEQAPTQ"
     gene            complement(16764..18050)
                     /locus_tag="VFV99_17955"
     CDS             complement(16764..18050)
                     /locus_tag="VFV99_17955"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HEX5061259.1"
                     /translation="MSARSAFFSLLVVLTITLAVICACEPIEGDAWGHYFAAQQSFSW
                     DHFVAVAKGSYLHGNPRWGQLVLATMFHAPIAAAVISALIIVAVLLLSMALIRARWPR
                     SRDPADGWLLVQVLATAILTTPQFGAIWFYRPNCTNYVYPLALQLAWLVPYRFLAARP
                     MASPWWLAFAMVPLGLLAGAGNEHTGVGLAVAAVVLTYIAWSRDRTLPVWSITGVLAL
                     AGGYAALLLAPGQHERYGGLANQQGTFERIADRGVLGNLSIIGVLVAWLSPTLVGTLA
                     LAGRRIVRVPPRAIVGFLAIAAVMIATTLAAPKVPIRLLAAPATMVALALGVFLVELA
                     TDRVKARWLRIASLAVSSLALATTLTIFVVTGIEGRARLHTLETAPPAATVCTPPYTF
                     AAPSPFSWGDDFRSPHLRKRIAQAYGIAQIDWTCAR"
     gene            complement(18194..18346)
                     /locus_tag="VFV99_17960"
     CDS             complement(18194..18346)
                     /locus_tag="VFV99_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061260.1"
                     /translation="MTITRRHRRTARKLVLPLIAVGLMTMMMYLHSGSSTQQGASNVT
                     NALYSR"
     gene            complement(18390..18734)
                     /locus_tag="VFV99_17965"
     CDS             complement(18390..18734)
                     /locus_tag="VFV99_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061261.1"
                     /translation="MKVVVVLAVLLASAEAFATVGRIDVAVDDTTRVPVGDAIGWFCD
                     DPSLIAASIVTQGTYNEWVVTGAKEGVTQCRIGNVASGRATVFIEVHVLPRSSELRRD
                     KQTSAPTRIAAS"
     gene            complement(18811..19467)
                     /locus_tag="VFV99_17970"
     CDS             complement(18811..19467)
                     /locus_tag="VFV99_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5061262.1"
                     /translation="MTEYAADLVDETLQRRARTLRADIESGALRGGELLAVLRDVPFV
                     DRDAWTDEVLGFAAPPPDVQLPRGSVPYLPCGVDEIMAMVEAVPLRPTDEVVDLGSGL
                     GRVVVLAHLLAGMKACGIEIQEHLVASARATAAALGLSAVSFVHANAADAVLDGSVFF
                     LFAPFNGEMLANVVRRLEDLAQERSFVVCTVGIELDAPWLRRRVASHRTLVIYDTITR
                     "
     gene            19612..20316
                     /locus_tag="VFV99_17975"
     CDS             19612..20316
                     /locus_tag="VFV99_17975"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5061263.1"
                     /translation="MYDAADVINDSTEAQMEAISRELFAKAGVAIVVVTVPELVDETI
                     EQLAVRIQHDWGVGTKGKDESVVIALSVADRQIFLATGYGSEPYLPDGKVGEIRDHAL
                     PALRANDYSRGLLTAVRETAKVAADAHGVKLLGDDSVGQSTTPTARTSEDTGCSDELV
                     SFAIIIVILLLLGGRRRGGRGGRRGGFIVGPWFGWGGGRSWGGGGDDFGGSGGGGGFG
                     GFGGGSGGGGGAGGRF"
     gene            20328..20894
                     /locus_tag="VFV99_17980"
     CDS             20328..20894
                     /locus_tag="VFV99_17980"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5061264.1"
                     /translation="MNNRKWLPVAVIVGAIVVIALVVGTKYNSLVSEREQVNGSWAQV
                     QNVLQRRNDLIPNLVATVKGYAAHEKETFTQIAEARAKLAGARTPSDAANANAQLDSA
                     LSRLLVIAEAYPQLKANENFVRLQDELAGTENRIAVERQRYNNEVRDYNRTIKRFPTN
                     LVARTFDFQRRDYFEADVDAQKAPEVRF"
     gene            20913..21902
                     /locus_tag="VFV99_17985"
     CDS             20913..21902
                     /locus_tag="VFV99_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5061265.1"
                     /translation="MATEPDEHEHEHDEHDEHDEHEHEHAHGSHAGHGHHAPPDKMTR
                     AFAIGIALNISFVVIGIIAGAVARSTALLADAAHNLGDVLGLGMAWGAAALARRATTS
                     RRTYGLRRTTILSALANGGLVLFAIGGVGWEAILRLKTPAPVDGGIVAVVATIGVVLN
                     GVSATLFAKGRKRDINVRGAFLHLIADAAVSGGVLVAGLLIWQTGWMWIDPATSLVVS
                     ATILFGSYKLVREALDLLLDAVPPHIDADAVETYLASLPEVRAVHDLHVWSMSTTEVA
                     LTCHLVLPWPAVQPTFQHTIQDELEHRFGIHHITIQFEDCVRVQCAQAAPESL"
     gene            complement(21904..23307)
                     /locus_tag="VFV99_17990"
     CDS             complement(21904..23307)
                     /locus_tag="VFV99_17990"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5061266.1"
                     /translation="MRFVWAGLLAVGFATSAYANGRDPGTSTINFRRDNDQHILAGMT
                     FGLVESRDGGATWQWMCERAVGYGGQYDPDYVYSSTGAIFATTFDGLKVRRNACTFAS
                     APPGMTFVARVKGGPDGAIYYAAADLADAKIYKSTDDGMTFPTSANPGQAGDWWSSLA
                     IAPGNAQRIYLTGYRLDGVNPKIFLLYTSTNGGTSYTAMNMTGITPVSANSTIDVVGI
                     SPTSDTTLYVKVTFESGGSGDSIYRSTDAGQSWTKILTRNSLYGLSFLVRTDATTCVA
                     GTRELGAWISTDCSTAASPTWTALAGAPHIGCLYNDSAGTVWACTQNYPSPTIPSDGF
                     GIMKTSDLSAWTGVLRFQDIQAPVSCPAGTVQEDQCVQRYMGNESVWCCLVNQLGITS
                     TAVDCTGALACFGMGPGDGPVDGPVDGAPNVDGAPDGGTTKMKPRDGCCGAGAGRSSL
                     MLGVLVAVVLLRRKRRA"
     gene            23439..23633
                     /locus_tag="VFV99_17995"
     CDS             23439..23633
                     /locus_tag="VFV99_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061267.1"
                     /translation="MRAVIAVAVVGLLACDQGVVAKPKPAVATVTPPAPPNAELTADG
                     PFTAAAWDLVAPLLTTHRNK"
     gene            23761..24063
                     /locus_tag="VFV99_18000"
     CDS             23761..24063
                     /locus_tag="VFV99_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061268.1"
                     /translation="MRSATPAITTELAAIAREITHLRRRVLLLHAAVVTGGVAVLVRA
                     FHGVSLTERSRFFAYWAAILVMSLVAFAISTRMRERLALLFARARFIEIRNRYGGE"
     gene            complement(24048..25028)
                     /locus_tag="VFV99_18005"
     CDS             complement(24048..25028)
                     /locus_tag="VFV99_18005"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5061269.1"
                     /translation="MRRHAAWLVLVLGASCGDDTTMQGDAAPSAWRLGPKIPARRLEP
                     GVTALGTQLVVLGGFDTGVSEGLHITDAVDVFDTETETWGPPLPPAPVQWSHIDLASN
                     GTTLFLLGGLEGEQFIARGDAWALDAGASAWRPLARLPAGLERGAAGVVSAPGHIYLL
                     GGASTTDALATCLDYDIANDSWTQLPALPGPRSHPAAMRTPDGTLLVAAGLRTIDSTK
                     PLSDVLALPPGGTAWEPRTATLLVPRGGCAYGALADQLICVGGESGNSALRGADLYDA
                     SLDRWSSLELLPVNRAGTQGAAVGTRFYVPGGAPALAYLPTDTLYIYSPP"
     gene            complement(25025..>25813)
                     /locus_tag="VFV99_18010"
     CDS             complement(25025..>25813)
                     /locus_tag="VFV99_18010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5061270.1"
                     /translation="QLIGWPDGLCDEIVGRGLHVIRFDNRDVGESTHLTSAPTPNFIA
                     AMAGDTSSASYSLSDMAGDSVGLLDALGIDSAHLVGASMGGFIAQTIAIEHPTRVRSL
                     TSIMSSTGDRSVGQPHPEGMQVFAAPPAFTREDVIKRSQNAFRAVGSTGFEVDLDAIG
                     ERAGRAFDRSFDPLGVMRQGVAVLASGDRTARLRSLDVPTMVIHGAVDKLCDVSGGRA
                     TAAAIPGAELLVIEGMGHDLPRALWPAFAAKIAETVKRGEARRR"
BASE COUNT         4436 a         8769 c         8448 g         4160 t
ORIGIN      
        1 atcgccggga actgcgcggc ggtccgcatc gcgacatcca tctgggtgtc gctgagcccg
       61 aacgtcttct tcatcgaggc gaggaactcg atctcgcgcc agtcgttctt gccgtcggcg
      121 agcgcgatga tcatcacgta gaccgtgacc cagtagcggt ccgacgcctg cgcgatttcc
      181 ttcgcgacta gggcgagctc ggccttgttg tccttgccgc tgatccgctc gagggcgacc
      241 tcgatcgcct cgtcgagctc ggcggtcgtc agcacgctca tgtcggggat gttgcgcagc
      301 acggcgcgca tcccgaggcg ctcctcgtga tggatctcgc cgtcggcggc cgccatcagt
      361 gccatgcact ttacgaacgc cggcatcagg aagcggacct tctccgactt ctcttcgagc
      421 gtgagcaggc ccgagtgctt gcgcgctgct tcgaagactt ccttcgcctc ggattcttcg
      481 agcgcgaact cgctctgcat cccgtcgagg aaatcgatct ccgattcggc aagctcgcga
      541 tcggcgacgc acacctcggc ggccatcgcg tacgcggcaa gccgctgatt gcgcgacggg
      601 agtcccgccg caatcgccgg taggcgcgcc ttgcctccac ctgcaacgcg aatcgcgtcg
      661 gcggcgatct ccgtcaatcg gccgagctct tctccggata gcccttcgaa aagcgggtga
      721 ttggcgaggc tgccctcgaa cgtcgccatt tcttcctcgg tgacgtcacc gtcggcttcc
      781 atcgcgccga gcatcgactc gaccaagaac cggcgcgggt cttcggcgct taccttgccg
      841 ctgaacattt tacgcagaaa gctcatcgtc ccctcacaat gaaggcctcg acggtatcac
      901 gcggacggtg ataggctccc tgcatgcgcg gcgcgatctt gctcgtcctg ttggctggat
      961 gcgtgatcga aggtcccgac gggatggacc gggggcccgc atacacccag gcggaaaaag
     1021 cctggctcgc cgaggcgctc ccggtgctgc aggccaattg cgtcgtctgc cattccgcga
     1081 gcgacgtccg cgggggcatc ggcttcctcg acggcgagac cgcctgggac atccgcagca
     1141 acctgctcgc atcgggcgaa gtgaacctcg accgcccgga gacctcgcgc ctgctcagca
     1201 aaggtgcgca ccagggtccg gcgatgaccg cgaccgaggc gtcgaaactg ttgttttgga
     1261 tccaagccga gcgcgacgag cggttctaac cggtcgtggc acgtaccatc atcggcgctt
     1321 tcatcatcgc gtttctcgtg ctgtgcgcgg tcaccacgta tcgcaacgtg ttcgccgatg
     1381 actccgcggt gcgtgcgatg gccgagaagc tcgcgcacga cacgggtggt tgcactggcg
     1441 actgcaagac aacccgcatc gaaggcaagc gcggcgtctt cagcaccgcg ttcgaattca
     1501 cgatgcagaa cggtgcgggc atcggcgtga cgtgtcggcg gccgtacgtc gcgttcggcg
     1561 actacgcgtg cacggcgacg aagcactaac gatcgaaccc gatcgaagcg gcgccgagcc
     1621 ggaactggcg ctgcacgtcg ggctcgaagc ggaacgcgcg caagaaatcc tcgacgctgt
     1681 acgccggcaa gcgctcgcgg atcctgccaa caaagcgctt tgcctcgtcg tatcgattcg
     1741 tgagcgccaa gcactctgcc gcgatcgcca gaatgtgcgc gtgcgcgttc gggcgcgacg
     1801 tcgcgcggac ggcccactcc gccgcttcta cgcgctcgtc gagccggaca tgagcgagtg
     1861 cgcggctcgc gagcatcgcg aattgcagcg gatcgaacgg gctgagctgc cgcgagtggt
     1921 tcgtcgcgtc gatcgccgcg cgcgggtcgc cgagctggct gtgaacgaaa ccgagcgtgt
     1981 aatgaccgag cgcgaagttc gggctcagct cgacgctgcg ctcgagctcg gcgaccgatt
     2041 cgctctgcgc atcgcgcagc cacaatgcgc gtcccatcgc ccagtgcgcc gcgggatcgt
     2101 gatcgtcggc cgacaagctg cgccccgccg tctcgaacgc gagctcgatc tggcgatcgt
     2161 gatcgggcgt gaggccgagg aacgcgttct gaaagtgcgt gaacgacagg cccgcatgcg
     2221 cgcgagagaa cgtgacgtcg agatcgagcg ccgcgcggaa gaactgctcg gcctggcgat
     2281 tgtcggcgcc ggtgaacttg tacatgtgcc agaggccgcg gtggtacgcc tcccacgcgt
     2341 cgagcgacga cggcggtttg agcacggcgc gcttgctctc tgcactctcg acctcgtcgg
     2401 cgatcgccgc gacgatgcga ttgacgatcg cgtcgagcac cgcgagcgtc tcgccggccg
     2461 gtccgggcag cgcgtcggtc cacaggatgc ggccgtcgcg tgcatccgaa agctcgacct
     2521 tcacgacgaa ttgcgtgcct tgccggcgca gcgagccatt gacgacgtag tcgacgccga
     2581 ggatgcgagc cgcttcctgc gcaccgacgc cacgctcggc gagcgagaac acactgccgc
     2641 gcgcgatcac gaacagcacg cgcagcttcg cgagctgcat gatgatgtct tcggtcaggc
     2701 catcagccac gcgaccatcc gccgccgacg cgtccacaaa cggcatcacc gcgatcgacg
     2761 cgcggcgcgc tgtcgtggcc tcgcccttcg gctgcacagc cgcgacttcg atccgcgctg
     2821 cacgcgtctc gcgcaacgct cgccacgcat cgcgtagcgg cacccaatcg atgccttctt
     2881 gttcgaacga gcgaatcgtc gtcgcgaggt gctgctcggc atcgtgatgt cggccgcacg
     2941 cgacgagcac ctcgagcatc acctcgtgtg cgcgcggatc gaacggcgcg atctgaagcc
     3001 agccctcgag atgccggaac acctcatccg agccaggtgc acatgccgcg agcgcgcgca
     3061 gtacctcgac atgcagtacg cgatagcgat ttcgctgcgc cgcgagccag ccggtgaact
     3121 cggggttgcc atcgatcatc acgccatcga gcaagtcgcc gctgaacagc tggcacagct
     3181 cggcgagccg cgcgtgggga acctcggcca tcccgccgcg cgccgccttg tcgagctcga
     3241 ggacatcgac gcggcagtcg gtcagatcga gcgcgacgtg atcgtcggcc gtcacgacgc
     3301 ggcgccgtgt accgtcgtcg agtaatcctc gcaatttgct caagcaccag cgcagctccc
     3361 cgcgcggatc gttcggcaca tcccacaaca ggtcgcacag ccgcgcgcgc gacaccggtt
     3421 gcgggctcag cgcgaggaac gccagtaatc cgcgaacttt gcgggagcga ggcaacggaa
     3481 cggcgacccc ggcgcgggtg atcgacgtcg ggccgagcac gcggacagcc atgcctgtcg
     3541 tttccacgct ctccaccacg gtccgtccca tgctcgggac ccatcttggc gcggttgccc
     3601 cgtgattccc agcccgaaac gtaggatgaa gcacccatga tctacgacga catcacgcag
     3661 acgatcggac acaccccggt agttcggatc aaccgcctcg cgcccaagca cgtgacgatg
     3721 tacgtgaaga tcgaggccgc caacccaggc ggttcggtca aagaccgcct cgcgctcggc
     3781 atcatcgaag acgcggagcg gcgcggccag ctcaagcccg gcggcaccgt cgtcgaagcg
     3841 acgtcgggaa acaccggcat cgcgctcgca ctggtctgcg cgtcgcgcgg ctatccgttt
     3901 gtcgcgatca tgcccgagtc gttctcggtc gagcgccgcc agatcatgcg cgcgtacgga
     3961 gcgaaggtcg tgctgacgcc cgccgccgaa cgcgcatccg gtgcggtgcg cctcgccgag
     4021 cagctctcga aggagcgcgg ctggttcctc gcgcgtcagt tcgataacga ggcgaacccc
     4081 gcgtaccacc gccagacgac tggccccgag atcctctccg actttgccgg catgcggctc
     4141 gactactggg tcaccgggta cggcaccggc ggcacgctca ccggcgccgg tcagatgatc
     4201 aagtgcgcgc gtcccgagac gaagatcatc gcgtgcgagc cggcagcggc cgcgctcctc
     4261 gacggcaagg aatggaagtc gcacaagatc cagggctgga cgcccgattt cattccgagc
     4321 gtcctcgatc gcgacgtcta cgacgaagtg ctcgcggttt ccgacgacaa agcaatcgcc
     4381 accgcgcgcg acctcggcat ccgcgaaggc atcttctgcg gcatctcggc gggcggcacg
     4441 tttgcgggcg cgctcgccgt tgccgagaag gctcccgcgg gcagcgtgat cctcgcgatg
     4501 cttcccgaca ccggcgaacg ttacctctcg acggttctgt tcgagggcgt gccgaccggc
     4561 tccgactcgg tctaacgaat cacgtcgtcg tggtgcgcga tgatcgcgca catcacttcc
     4621 tgaatctccg gatcgggtac gccgtacttc ttgagcgtgc gcacgatctg atcgccggcc
     4681 gccatgaagt gcacgtcggt catgtggagg tgcgcgtgcg ccgagaccat gtcgcgaccc
     4741 gtgtacttct ccttgccgcc acccgcctga cacagccaat cgacgacgtg gccaatgagc
     4801 ttcgccttgt cgacgccctt gcacagcggc gcggtgagcg gctcggtgaa gtgaagctcg
     4861 acgatgtctg tcacgaccgc ggtgatcggt ccgcggccgc cgagtcgctc gtacagcggt
     4921 ttcgctcgct ggcgttccgc acgcgctgct gcgctcgcgt cgaacatcgc cttgagctcg
     4981 gctacctgtc cctgtgcctt cttgatcgcg accgggtcgc gcggatccgt aacgtcttgc
     5041 attgctagct cctctcggtg gttggttacc gaggctcacg ctcggacacc gtcatgggag
     5101 cccgcgtgga agccgccgtg gaaacgggat cgagccatgc ttccgccagc ttggggtgtg
     5161 tcaccggcgc acggactgtc acgttttgtg gcttgtctcg ccgcctacgc gtagccgata
     5221 ctttcgggca tgtgcaggtg gctagtggcc gggctgctcg ccggctgtta cgccccggcg
     5281 ccaccagccg gcgccccctg cgtggacggc gtgtgcccga gcggcctcgt ctgctcgccg
     5341 gcgacgaaca cgtgtgagaa ggagtccgtc gatccgacgc gcgatggctc gatcgatggg
     5401 ccgaacccga tgatcgatgc agcgatcgac gcaccgaagg acggctcgac atcgccgttc
     5461 atgtatcgcc ggcgcatcac gatccaagcg ggcgctgccc tgcccgctgg ctacacgatt
     5521 cgcgtccagc tcaacaacat tctcgggacg ctcgtcagtc agggcaaagt gaagggcgat
     5581 ttctctgacc tgcgcgtgat cggcgatgcg ccgctcgggg agcgcaatcg catcatcgac
     5641 ggtgcccccg cgccagcggc gatcaacttc tcgctgcagc aatcgatcgc cgcgagcacg
     5701 acgaaccagt cgtactacgt ctactacggc aatccgaacg ccaccgcagc gcccgcgaac
     5761 ggtacgcagg tattcacgct ctacgacgac ttcaattccg gcatcgcgag cttctggctg
     5821 aagaacgacg cgccaacgac cacgggcggc aagctcgtgc tccgcgcggc acacaccgat
     5881 gcgatcacga cgaacgccgc gagcgatggc gtgccgatcg tcagcgccgt cgagctctcc
     5941 gcgtcgacca caaacccgac cagcgatccg acggtacaac ccgaggggac gttctattac
     6001 tggtggggct accagcacac cggcgacttc tcggcgagct caccatgggt catctggatc
     6061 gcgcgtggca aaggcgcggt tcacgccgag cacaatggcc cggtcggctg cgaagccggc
     6121 tgcactggcc ccgacctcgg ccagaacacc tcgctgcact actacgcgat cgagcgcgac
     6181 acgaacgcga cgcgcttcta cctggacggc acactccagt acacggcgac cgtcacgaac
     6241 aacgcggatt actcgctgat ggtgcgcaac taccaggccg ctggcgacgt gcaggtcgat
     6301 tggatccgag cgcgcgctcg tgtcacacca gagcccgccg tcacgctcgg cgccgaagag
     6361 atgctgtagg atccaagcgc taaggtccga atcggcgatg actccggata cgccacacgg
     6421 cacctggatc cataccgacg ttccgccacg cctcgacgcg ctcggttggt cacgttggca
     6481 tcgtcgcgtt gtcatcgcgc tcggcatcac gtggatcctc gacggcctcg aagcgagcct
     6541 ggtcgcgaac ctcgcaccga cactgatgca tcccgacacg ctcgggctca gcggcgccga
     6601 ggtcgggttc gcgaacaccg cgtacctaat cggccaggtg ctcggcgcgc tcggcttcgg
     6661 ctatctcacc gatcggctcg gccgcaagcg gctggtcctg atcacgctcg cgttgtatct
     6721 ggtgtcgacg gcgctgagcg gcttcgcgcc gaatttcgcc gtattcgtgt tcttccgctt
     6781 cttcgcgggc gcaggaatcg gcggcgagta ctcggcgatc aactcggcga tcgacgagct
     6841 ggtgcccgca cgtattcgcg gccagatcga cctcggcatc aatggcagct actgggttgg
     6901 cgtcgcgctc ggcgccggcg tcacgctgat cgtgctcgat ccggcgatcg tcccggtgtc
     6961 gatcggttgg cgactcgcgt tcgccttcgg atcgctgctc gggctcggca tcctcgtgat
     7021 ccgccgcgac gttcccgaga gcccgcgctg gctgctcatg cacggccacg tcgccgaagc
     7081 gaacaagacg atcgctcgca tcgaggcgca agcgcacggc gagctcgttg tcgataccgg
     7141 cgtgcgtccc gctcctgtgc gcttcaaggt gacgggaccg gtcggcctgc gtcacctcat
     7201 tcacacgttg ttcgtgcgct atccgcgccg caccgtgctc ggcctctcgc tcatgctcgc
     7261 gcagtcgttt ctctacaact ccatcttctt cagctacggc ctcattctgc agaaattcca
     7321 ccacgtcgat cccgagcgcg tcggcctcta catcgtgccg ttcgcgatcg gcaacttcct
     7381 cggcccgctc acgctcggtc acctgttcga tcgctggggg cggcgcacga tgatccctgc
     7441 gacgtacgcg ctgtccgcga cgctgctcct gttgaccggc gcgctgtttc tcggcggcta
     7501 tctgactgca ctcacgcaag cgctcgcgtg gagtgcggtg ttcttcgtcg cgtcggccgc
     7561 cgccagctcg gcatacctga cggtcagcga gctgtttccg ctcgagctcc gcggcatggc
     7621 gatcgcggtg ttctacgcgt tcgccacgct cgtcggcgcg ggcgcaccca cgctgttcgg
     7681 agcgatcgct gataccggcg agccgaacca gctgttcgcg ggctacgcgc ttgctgccgg
     7741 cctcatgtac atcgccgcga tcgtcgcgcg caagttcggc gtcgcggccg agggccgatc
     7801 actcgaagac atcacggcgc atccagagca gccggattga ctagcggcgg aacctgcgaa
     7861 cgaacagcac gacgcccgga atcatcacgg cccagatcag cgcgagaccg gcgagcgcga
     7921 gccacttgtc gatcgtgcgg ttgccggtcc actgcaaata gtggatgcga tacagccagt
     7981 cgatgcgatc ggtgtcgcgg ccgcgctgcg acgcgcgtgc atcgtgcacg ccaagctcga
     8041 cgaccgggcc gccacttgtc atcacgcgcg cggtgtcgcc cgcgatcgcg acgttcgtga
     8101 tctcaccgta ggcggcgcgg aactgcgagc gctcgattgc atcggcgatg agcgtgcgca
     8161 cggcagtcgc atcgagcgtg cggggcttgc cggtggcggc gtcgtagagc tcggtgccgc
     8221 tcgaggtgcg cacgcgatag agcggaccga tcgcggtgtc gaacagctcg atgcgcgtga
     8281 tcgacgagcc gaggttctgt ggcggcgcga acgtctgcag cggcagcacc gagtcgcggc
     8341 gctcgacgct gagttgatcg tacgcgcgat cccagcccgg cttgatatgg aacagaaggc
     8401 ccgagaccga ccacacgacg agcaagctga cgagcacgat cgcgagccag cggtgcgtcg
     8461 tcacccagaa tttggcccac aacatggcgc gccgttcata gcgcgtttgc tccgggcgat
     8521 gcgcgttcgt tacgccaggc cgatcaggcc gcgcttgcgg agcttgcgca gctgcgagcg
     8581 gagctgcttg tcttcgagtc gttcgcgcag gccgggtcgt gcgtcgagat aggcattcca
     8641 tgccacgcgc ttgaacacct caccggcgag cccgttcgcg gcagcgagct tcgctgctcg
     8701 atcgagcagc gcgatgagct cctccagcca cgcttcgggc ggctgactgc gcggcacctc
     8761 ggtcagagct ggattgggtt gcgacatgcg agcaatagaa gcacgaagcg gccgtttggc
     8821 gacccccgat cgggtagatc tcggcctcac agatcggtta gcgcagtagc cgcttcagca
     8881 gactcggccg ctgtgcgcgg ccaccgccac gctcgagggc ggcacgcatg tcagtcgcgt
     8941 tcgcgaaacg cttctcgcgg tcaaaagcga ggctgcgcgc gacgatatcg tcaagccacg
     9001 ccggaacttc agcgcgccga accgccggat gccgctcgag ctgacccttc atcacgtgct
     9061 tgagcagctc ccgttttgac gaccaggtgt acggccacga gccggtcatg ttctcgaaca
     9121 tcacgacgcc gagcgagaac agatcggcgc ggccgtcgac ctggcgcggg tcgaagcact
     9181 gctccggact gatatagagc ggcgtcccga gtagctgctc ggcatcggtc agctggtgct
     9241 cgtcggtcgc cttcgcgaac ccgagatcga gcaggcgcac gcgatcgctg ccggcctcga
     9301 tgaagatgtt ggcgctcttc agatcgcgat ggacaacgcc gcgcttgtgc agcgcctcga
     9361 gcccgtgcag cacctcgatc gcgatcttca ccatgcgcgc tggatcgatc ggcccttgct
     9421 cttcgcggat cgaccacagg tccctgccct cgaggcgcgc catctcgatg aacgcgcagc
     9481 catcctcgga gacacccgcg tcgtggacgg cgacaaatgc tgcgtgtcca atcgcggccg
     9541 tctcgcgtgc ctcgcggacg aaccgcttcg cgagctcttc gtccttcgcg aaccgcgggt
     9601 gcaagatctt cagcgcgacg tcccgccgca gcaaccgatg atgcgcttcg tagacggccg
     9661 cgatgccgcc gcagccgatg agcgagacga gctcgtactt gttcgcgatg accttgccga
     9721 cgcggctctt cggacatgtc tccgggccgc cgtcgtggtc ggcgccacac atctgacagc
     9781 cggccatagg accagcatat cgcgatcggt cagcgccgtg tcgaccgtcg acggcgccgc
     9841 agcaggagca atccgccacc gagcaaccac gaggcgtcgg gcatgccacc gacgccgcat
     9901 cctgcgcagc cgccgccgac gctgaagttg aacgtgcacg tcgacgtatc gcacagctcg
     9961 ccggtctcgc agtcttcgcc ggcggcagcg ttgacgtagc cgtcgccgca cgccgacgtg
    10021 aggcacgtcg cgccgttgca cgtttcggag tcgacacccg acgaatcgca ctgctcgcca
    10081 gcgaccgtgt tcatgtagcc atcgccgcat gccgacgcgg tacaggtcgc gccgttgcag
    10141 gtcggcgtgt cggcgccgtc gcacagctcg cccgagtcga cgacgccgtt gccgcagttc
    10201 gcgctgacga agcacgggat catcgtcgtg ccgtaatagc ggcgcgcgtt gatcgtctcg
    10261 ctggcgccga ccgcgagcga gaagtggtac tcgatgccga tcgtcgcgtc accgatgacg
    10321 ctaccgctct cgccgacggc gttcttgccg ggaccgacga catagttctg ccactcggct
    10381 ggcatgccgt ggtcaagcca gatgtactgc tgaccgatcg ctgcgttgtc gggaacgaac
    10441 gcaggcccgc tgcccggcac gaagccatcc ttgccgccgt agtagtacgt gagcggaacc
    10501 gtcgagagcg gaccgttgcc gagcccgatg ctccaccgtg gatcgccgtc gtgctggtag
    10561 acgccgcaca ggcccgggcc cacgccgacg acgtcgtcat cgtccttgtt cgcgccgttg
    10621 tagaacagat cggaatccca catgaagtgc aggacgagcg acaccgggac cgtgccgttg
    10681 ttcgtgaacg tgtagacgta gtcgagctgg ctcgaccgcg cgaccgcgtc cgaggtcagc
    10741 gactggacga gggcgacgtc gatgcgaatt ccaccctggg catcggtcgc gagccgaaac
    10801 gcgctcgtag cttggtttgt gcccgtcatc gcgatcgcgg tcgtcaccga gcgtgcgagc
    10861 gccgtgtgca tcgggtcggc gagaatcccg tcaggtgtcg gcggcgggtt ctcgacgaaa
    10921 tcggcccaga ccttgtagtc ggtcagcagt accgagctcg tgtggttgcc ggcggtcgtg
    10981 atgaacagct cggggcccgt caagtgcgtc ggcgagaatg ggttcgagac gcccggggga
    11041 tagtattcgt cgtcgtactg ctgaccgatc gcaaagccat gggtgccgta gtcatcgggc
    11101 gtgaccgacg tgaagccggc gccggtaacg ccgttgtcga acgtgaccgt gcccgcagcg
    11161 acgttgcgtg cgcttataca tgcgatcgcg atagcgcacc cacagcgcgc catcaccccg
    11221 agtttcccca cgcggccagt ataggcgcgc agcgagtgct acgatagctc gcgatgctgc
    11281 caagggggtt ggcggcgctc gcggttgtag cggcacttgc tggaaccgcg cgcgcccaag
    11341 aggcgacgga ccagctcggg atcgagcggt tccggatcgc gatcgatcgc gccggcatcc
    11401 tcgacgtcga gtgggccggc gtccctgggc atctctcatg gggtgccggt gtgctcgtcg
    11461 gcttcgcgca cgatccgctg gtcgtgtacg accgcgcgat gagcgccgtc gatgcgctcg
    11521 tcgatcggcg gctcaccacg accgtcgttg gctcgatcgc gctgtggaat cggctgcagc
    11581 tcgggctcac cgtcgacatc gtcggctacc agagcggtga ggacgtgctc gccacaatgg
    11641 gcgcactacc gacgagcggc ctgggcgaca tgcgattgct cgcgaagctg ctgatcgcag
    11701 gcgacgcgcg gtatcagctc gcgctgatcc ccgcgctcac cgtgccagcg ggtgaggcgc
    11761 gcggttactt gcgcgagtcc ggggcagcat ttgcgcccgc gctcgccgct tcggtgcggt
    11821 cgggccgcgt gcgtggcgca ctgaacctcg gatacgcgat caagccgcgc gtcgaggccg
    11881 ccgggctcgt cagcgatgac gaagcatttg cgcgactcgg cgcaggcgtc acgctcggtg
    11941 cacccgcggc acctgccgcc gagctgtggc tggcgacatc gatcgcgaca ccgctgaccg
    12001 atgcggcgca caaccaggtc gcgatcgagc tgctggccgg tgccgcccgc cgcttcacgc
    12061 cgacgctcga gggcttcatc ggcggaggca tcgggctgga caacggtttc ggcacgccgg
    12121 actggcgcgc gctcgcaggt gtgcgcttcg aggtggcgag cggcgatcgc gatcgcgacg
    12181 gcgtcgctag cgaggcagat cgttgccccg acgaaccaga ggaccgcgac ggcttcgcgg
    12241 attcggatgg ctgccccgac ccggacaacg acggcgatgg cgtggccgat gcgcgcgatc
    12301 gctgcgcaaa cgaggtcgaa gacaacgatg gctttcagga cgatgacggc tgcgcggatc
    12361 gcgacaacga cggcgatggc gtcgccgacg cccaggaccg ctgcccagac aaaccggaag
    12421 acaaggatgg ctttcaggac gacgacggct gtcccgacga catcgcgcgt gtcgatggtg
    12481 tcgtcgtcga tcccgatggc cgcccgctcg ccggcgcgaa gatcagcatc gtccagtacg
    12541 cgaagccaag cgccgcgccc atcgagctca ccgccggcga cgacggcaag ttctcgacgc
    12601 aactcgacgg cggcgccctc gacgtcacgg cctctgccgc cgagtacaag accgggacga
    12661 ccagcgcgac ggtcgagcca ggcacacacg ccgatgtcac cgtgaagctc gtgcgcgccg
    12721 tgcgtcaggg tcagctgcgt ggccaggtgc tgtcgttcaa cggcaagcca ctcgccgcga
    12781 cgatcaccgt ccgcggcaag accaccacga gcgcgacgac cgacgcggac ggccagttca
    12841 cgatcgagct ccccgaaggt ccgttctcgg tcgaaatcgc gtcgcccggt ttcgtcacgc
    12901 agaagcgcaa cgtcacgatc aagctcgatg gcgtcaccgt gctcaacgtc gatctgcggg
    12961 gcggctcgtg atccggatcg tcgtgttcgc tctcgcgctg tgtgcgtgca agaagtcgcc
    13021 cgatccggtc gtcgcacggc tcgatgcgat caccgcgcac gtcgagcgca tgccgcgcga
    13081 ggccgcgccg tggcaaaccg cgcgcgtcgg cgatggcttc gtgatcggta gcgccgtgcg
    13141 cacgggtgcc gcgtcgcacg cgaagctgac cgtcggcaag tcgggcaagc tcgacgtcga
    13201 cgcgaacgcg gtcgtctact tcacccgcac gcctgggcgt gatcgcagcg acctgcgcgt
    13261 cgagaccggc gtcgcggagc tcgagactgg cgaggagacc ttcggtgtcg gcgaggccac
    13321 gctcgatccg aacacgcacg tgcgcatcgc ggcgagcccg cagggaacct cgctcgtcgt
    13381 cacggtcggg cgcgcgacgc tcgacgacac ggtcatcgcg gctggccagc agatcacgat
    13441 cggtccggcc ggtaaacaag tggcggcccc gcccgacgct ggcatcaccg cgcccgccgg
    13501 ccagatcgcg atcgcggtgc gcgacaagcc ggcgcgcgta acggccggtg gcaccgcgac
    13561 ggagctcgct gtcggcgagc atctcgtggc cgatggcgcg acgctcgacg tgcccgccgg
    13621 cagtacggtc gaggtcagcc gcggcggtgc gcgcgcggtc acgactggac cgagcgtcct
    13681 gcgcgtcgga gataaggaag cactcgtcgc cgttactgcg ggcggcatcg cgctgcacgc
    13741 ggagaccgcc gacgcgatcg cgatcgtccc aggcggcacc gtcaccgcga agcagggcgg
    13801 cgatgtggcg cttgacgtcg atgcgaagga gactgcgatc gatgcgcagc gcggcgagac
    13861 cagcgttcag acgccgcgcg ggacgcaatc gctcgcggcc ggccagtcgg cgctgctcgc
    13921 gaagtccggc gagatcacac tcgtccctgc cccacccgcg cgctcggtcg tgacgatcac
    13981 cgcgggcgaa tcgccaacgc tgcacgatcc gcgcgcgcca acgccgctgc gggtcgcgtt
    14041 cgatcaggcg tgtccgtcgg gtggcaccgt cgaggtcgcg aaggaccgcg cgttcaagaa
    14101 aatcatcgcg cgctcgggcg gcacgggcgc cgcgaacgtg ctcgtgcccg ccggttcgtt
    14161 caattaccgg gtgcgttgcg ctggcggtcg tggggcgagc ggaacgctgc gcgtcgcgaa
    14221 ggactcgggg cgcacgccgc tgccgaaggc tgccgcgcgg acgactgtcg agatggacgg
    14281 ccgcgagtac acgatcctct atcagaacct gctgcccgag ctgacgctcg cgtggaagac
    14341 ggcgccgcgc ggcgcgaagt acacgttcgt cgtcaagccg ggccgcggcg ccgacgagcg
    14401 catcgcgagc gcgacgccga cgaccaagct cgccgccggc gacgtccgcg aagggagcta
    14461 caaggtctgg tgcgaagccg acggcggacg acgctcggag gagagccgca tcgtcatcga
    14521 cttcgataac gcggcgcagt cggcatcgat cgacgcggtc gaggtcagcg gcacgacgat
    14581 gcggatcaaa ggaaccgtga tcgaaagttc cacggtcact gcacacgacg ccgcgatcgg
    14641 gctcgatcgc caccgccgct tcgatgccga attaacgacg cgcgagggtg aggacggtgt
    14701 cggtgtgcgg atcgcgcacc cgaaggcagg gatccactac tacgtgatgc gcgccggggc
    14761 gtcgtagctt acgatcgcag cgatgttggg agagacgctg ggcagctacc ggatcgtttc
    14821 cgaaatcgga agcggcggta tgggcgtcgt ctatctcgcc gagcacacgc tgatcggccg
    14881 caaggtcgcg gtcaaactgc tgcgcgccga catgccgtcc gagtacgtcg agcgcttctt
    14941 caccgaggcg aaggcggccg cccagctgca ccacccgggt ctcgtcgacg tgttcgactt
    15001 cggccatcac aaggacggcc gggcgtacat cgtgatggag ttcctcgaag gcgagtccct
    15061 agcggagcgt ctcgcgcgcg agccgcggct gccggttcca ctcgcgtgca tcatcacgcg
    15121 cggcgtcgcg atcgcgctcc atgtcgccca cgagaagggc atcatccacc gcgacctcaa
    15181 gccagggaac atcttcctcg ttccggatac cgaggcgcct gccggcatcc gcgcgaaggt
    15241 tctcgacttc ggaatcgcga agctggtgcg tgaccgcgag gagcgctcgg tcaagaccgc
    15301 gtcgggcgcc gttatcggca cgccgcgcta catgtcgccc gagcagtgca agaacgcgcg
    15361 caccgtcgac gggcgcacgg acatctactc gctgggctgc atcctctacg agatgctgct
    15421 cggcgtcgcg ccgttcgact acgacagctg ggccgagctg gtcggtgcgc acctctacga
    15481 ggttcccccg aagccgacgg agatcgatgc gaaggtgccg gcggacgtcg agacgctcgt
    15541 caccaagatg acggagaagc tgcccgccga ccgcttccag tcgatgcagg atctcgcgca
    15601 gtcactcgag gtgatgatcg cgacgcatgc cggcgaggcc gggcgactga cgccaacgtc
    15661 gttgcagcga gctgcctcca ctccaccgag cggcgtgcgc aagctcgaag gtgcgagcga
    15721 tccgacaatc ccgggctcgt ccgccgagat gatggccgtc cgcgcaccaa cgactggctc
    15781 gggttcgaag aagtcagacg cgatcacgcc aacgctcagc gccgagatga cgcccgcgaa
    15841 gagcgcgccg cgcaagctgc cctgggtcgc gatcgcgatc ggcggtgtcg gcatcacggc
    15901 gctcgcgatc acagcgttcg tcgtgctcgg caaggacacc aaaccacagc ccgagaccgc
    15961 gtatatcgtc gtcgatcagc gcggttctgc ggaggcggtc ggtagcgcgg ccgcaaagcc
    16021 cgtcgaagac tcgccgccga cggtcaactc gccgccgacg gtcgacgctg gcgtcgccga
    16081 ggtccacgag accgggtcgg ccgtcttacc caagatgccg aagacgaccg atctcgacgc
    16141 gctcacgcgg acgttcggca agcagtcgcc ggcgatcacg gcgtgcttca agaagcaccc
    16201 cgcgacatcc gagcaggtat cggtgcggat ccagatcgat acgcgcgggg tcgtgaaggc
    16261 agccgaggta ttgcccgaaa ccgtcggtgc aagcccgctc ggcgcatgca ttgccgatgt
    16321 cacccgcaag acaagcttcg gtctacagcc gaaaccggcg acgttccggg tgccgctggt
    16381 caagaacgag cgatagctgt tgctggacgt tcgtgccgag gtagcctgta gctatgaagt
    16441 cgttgttgtt cgtggtgaca gtcgcggcgt gcggcgggcc ctcgcagcaa cagatcgtcg
    16501 aacagccggc tgcgacggca ccgttcccgc acacggaagc gccgcctgca agcacctctg
    16561 atcaagaccg ttcgcatctg aaccagcagt tcgaagacat gcaatggacg caggatgcgt
    16621 atcggcaggc cgaaggtgct gaacgcgcaa agacgccgcc tccgaagaaa caagcagcgc
    16681 cgccgaatcc gaaaaaggcg cccgtcgagc aagcaccgat gccgaagaag aaagcgccgg
    16741 tcgagcaagc gccgacgcag tgatcagcga gcgcaggtcc aatcgatctg tgcgatgccg
    16801 tacgcttggg cgatgcgctt ccgcagatgc ggactgcgga agtcgtcgcc ccacgagaac
    16861 ggcgaaggcg cggcaaacgt atatggcggc gtgcagacgg tcgcagccgg tggcgccgtc
    16921 tcgagcgtat gcagccgagc gcgcccttcg atgcccgtga cgacgaagat cgtgagggtc
    16981 gtcgcgagcg cgagcgatga gaccgcgaga ctcgcgatac gtagccagcg cgccttcacg
    17041 cggtcggtcg ctagctcgac gaggaacaca cccagtgcga gcgcgaccat cgtcgcaggc
    17101 gctgcaagta accggatcgg aacctttggc gcggcgagcg tggtcgcgat catcaccgca
    17161 gcgatcgcta ggaagccgac gattgcacgc ggcggaacgc gcacgatgcg gcgtccagcg
    17221 agcgcgagtg tcccgacgag cgtgggactc agccacgcga caagtacgcc gatgatgctc
    17281 aggttgccga gcacaccgcg gtcggcgatg cgctcgaatg tgccctgctg attcgcgaga
    17341 ccgccataac gttcgtgctg gccgggcgcg agcaagagcg cggcataccc gcctgcgagc
    17401 gcgagcacac ctgtgatcga ccacacgggc agcgtgcgat cgcgcgacca ggcgatgtac
    17461 gtcagcacga ctgccgcgac ggcgagaccg acaccggtgt gctcgttgcc cgcaccggcc
    17521 aagaggccga gtggaaccat cgcgaatgcg agccaccacg gcgacgccat cggacgtgcg
    17581 gcaagaaacc gatacggcac gagccacgcg agctgcagcg cgagcggata gacgtagttc
    17641 gtgcagttcg gccggtagaa ccagatcgcg ccgaactgcg gcgtggtcag gatcgccgtc
    17701 gcgagcacct gcacgagcaa ccagccatcc gccgggtcgc gcgaccgcgg ccagcgcgcg
    17761 cggatcagcg ccatcgacag caacaggacc gcgacgatga taagcgcgga tatcaccgct
    17821 gcagcgatcg gcgcgtgaaa catcgtggcg agcacgagct gtccccagcg cggattgccg
    17881 tgcaggtagc tacccttcgc aaccgcgacg aagtggtccc acgagaacga ttgctgcgcg
    17941 gcgaagtagt gcccccacgc gtcaccctcg ataggttcgc aggcgcagat caccgcgagc
    18001 gtgatcgtca gcacgacgag taagctaaag aacgccgaac gtgcgctcac gcaccgcgtt
    18061 cgtgcaatcg tcgcaccgct cggcaagcga tgcgtgcgaa gcgattaggc cgcgcgcgcc
    18121 tcagaatttc gtcgcgtgtc tgacgccgag gtccgaggtc gttcgctgac tggcacctgt
    18181 acgtgtcgcg agctcagcgc gagtagagcg cgttcgtcac gttcgatgcg ccctgctgcg
    18241 tcgagctgcc cgagtgcagg tacatcatca tcgtcatcag accgaccgcg atcagcggca
    18301 gaacaagctt gcgggcggtg cgccgatggc gtcgcgtgat cgtcatgcac cgagaagatc
    18361 tcgaaaccgg attcgagaca acttttcggt cagctcgcgg cgatgcgcgt cggtgcagac
    18421 gtctgcttgt cgcgccgcag ctccgagctg cgcggcagca cgtgcacttc aataaagacg
    18481 gtcgcgcgac ccgaagccac attcccgatc cggcactgcg tgacgccttc cttcgcaccg
    18541 gtgaccaccc actcgttgta cgtaccctgc gtcacgatgc tcgccgcgat caggctcggg
    18601 tcgtcacaga accaaccgat cgcgtcgccg acgggcacgc gcgtcgtatc gtcgactgcc
    18661 acgtcgatgc ggcccacggt tgcaaacgct tccgcgcttg cgagcaacac cgcgaggacg
    18721 actactacct tcacacttcg atcgtagcgc cgccgcagcg cgcaacaacg tgcgcacgag
    18781 ccctgcgcga acaacgtgac agcgttactc ctacctggtg atcgtgtcgt agatgacgag
    18841 cgtgcgatgc gaggctacgc ggcggcgcag ccacggcgcg tcgagctcga taccgaccgt
    18901 gcacacgacg aacgagcgct cctgcgcgag gtcttccagg cgccgcacga cgttcgccag
    18961 catctcgcca ttgaatggtg caaagaggaa gaacaccgat ccatcgagca cagcgtccgc
    19021 cgcgttcgca tgcacgaacg acacagctga caaaccgagt gcagcggccg tcgcgcgcgc
    19081 gctcgcgacg aggtgctcct ggatctcgat tccacacgcc ttcatgcccg cgagcagatg
    19141 cgcgaggacg acaacgcggc caagcccgga acccagatcg acgacctcgt cggttggccg
    19201 aagcggtacc gcctcgacca tcgccatgat ctcgtcgaca ccgcacggca gatacggcac
    19261 cgaaccgcgc ggcagctgca cgtccggcgg cggcgccgcg aacccgagca cttcgtcggt
    19321 ccaagcgtca cgatcgacaa acggcacgtc gcgcagcacc gcgagtaact cgccgccgcg
    19381 cagtgcaccg gactcgatgt ctgcgcgaag cgtccgcgcg cgacgctgta gcgtctcgtc
    19441 cacgagatcc gcagcgtact ccgtcatgcg agaaggccct agcgggcagg cgtactggtt
    19501 gcaacggact catgcatgcg ggctgtcctg ctgaccttgg caatcttggc cgccgtcggc
    19561 gcgcgggctg ggcacgccgt gacgcccctg ccccagcgcg cagatcggtc agtgtatgac
    19621 gcagccgacg tcatcaacga cagcaccgaa gcccagatgg aggcgatcag ccgcgagctg
    19681 ttcgcaaagg cgggtgtcgc gatcgtcgtc gtcactgtgc ccgagcttgt cgacgagacg
    19741 atcgagcagc tcgctgtccg tatccagcac gactggggcg tcggcaccaa gggcaaggac
    19801 gagagcgtcg tgatcgcgct gtcggttgcc gaccgccaga tctttctcgc cactggatac
    19861 ggaagcgaac cgtatctacc cgacggcaag gtcggcgaga ttcgcgacca cgcgctgccg
    19921 gcactgcgcg ccaacgatta ttcgcgcggc ctgctcaccg ccgtgcgcga gaccgcgaag
    19981 gttgctgccg acgcgcatgg cgtgaagttg ctcggcgacg atagcgtcgg ccagtcgacg
    20041 acgccgacgg cgcgcaccag tgaggacacc ggctgcagcg acgagctggt gtcgttcgcg
    20101 atcatcatcg tgatcctgtt gctgctcggc gggcgtcggc gcggcgggcg cggtggacgc
    20161 cgcggcggtt tcatcgtcgg tccatggttc ggctggggcg gcggccgcag ctggggcggt
    20221 ggcggcgacg atttcggcgg cagcggcggt ggtggtggat ttggtggctt tggcggtggc
    20281 agcggaggcg gtggtggcgc aggaggccgt ttctgacagg agaacctatg aacaatcgca
    20341 aatggctccc agttgcagtc atcgtcggcg cgatcgtcgt gatcgcactc gtggtcggaa
    20401 cgaaatacaa ctcgctcgtc agcgaacgcg aacaagtcaa cggctcgtgg gcgcaagtcc
    20461 agaacgtgtt acagcgacgc aacgacctca ttccgaacct cgtcgcgacc gtcaaaggct
    20521 acgccgcgca cgagaaggaa acgttcaccc agatcgccga ggcgcgcgcg aagctcgcgg
    20581 gcgctcgcac gccgagtgat gccgcaaatg caaacgcgca gctcgactct gcgctgtcgc
    20641 gtctgctcgt gatcgccgag gcgtatcctc agctcaaggc gaacgagaac ttcgtgcgtc
    20701 tgcaggacga gctggcgggc acagagaacc gtattgccgt cgagcgccag cgctacaaca
    20761 acgaggtccg cgactacaat cgaaccatca agcgattccc gaccaacctc gtcgcgcgca
    20821 cgttcgactt ccagcgccgc gattacttcg aggccgacgt cgatgcgcag aaggcccctg
    20881 aagttcgctt ctagcgcgcc acacaggcac cgatggcaac cgaacccgac gagcacgagc
    20941 acgagcacga cgagcacgac gagcacgacg agcacgagca cgagcacgcg cacgggtcgc
    21001 atgcaggcca cggccatcac gcgccgcccg acaagatgac gcgagcgttc gcgatcggca
    21061 tcgcgctcaa catctcgttc gtcgtcatcg gcatcatcgc gggtgccgtc gcgcggtcca
    21121 ccgcattgct cgccgatgct gcgcacaacc tcggcgacgt gctcggcctg ggcatggcct
    21181 ggggcgctgc cgccctcgcc cgtcgagcga ccacctcacg ccgcacctac ggcctacgcc
    21241 gaacgacgat cctgtccgcg ctcgccaacg gcggtctggt cctgtttgca atcggcggcg
    21301 ttggctggga agccatcttg cgcctcaaga caccggcgcc ggtcgacggc ggcatcgtgg
    21361 cagtcgtcgc aacgatcggc gtcgtgctca acggtgtcag cgcgacgttg ttcgcaaagg
    21421 gccgcaagcg cgacatcaac gtgcgcggcg cattcctgca cctcatcgcc gacgcggcgg
    21481 tctcgggtgg cgtgctcgtt gccggccttc tcatctggca gaccggctgg atgtggatcg
    21541 atcccgcgac gagcctcgtg gtgtcggcga cgattctctt cggcagctac aaactcgtgc
    21601 gcgaagcgct cgacctgttg ctcgatgcgg ttccgccaca catcgatgcc gacgcagtcg
    21661 agacctatct tgcctcgcta cccgaggttc gcgcggtcca cgatcttcac gtgtggtcga
    21721 tgagcacgac ggaagtcgcc ctgacgtgcc acctcgtgct gccctggcct gccgtgcagc
    21781 ccacgttcca gcacacgatc caggacgagc tcgagcatcg cttcggcatt caccacatca
    21841 cgatccagtt cgaggattgc gtccgcgtgc aatgcgcgca agccgcgccc gagtcgctct
    21901 aggtcacgcg cgtcgcttgc gccgcaagag aacgacagcg accaacacgc cgagcatcag
    21961 cgacgagcgt cccgcgcctg cgccgcagca accgtcgcga ggcttcatct tcgtcgtccc
    22021 gccgtcgggt gcaccgtcga cgtttggtgc gccgtcgaca gggccgtcga caggcccgtc
    22081 acccggaccc attccgaagc acgcgagcgc gccggtgcaa tcgacggccg tcgacgtgat
    22141 ccccagctgg ttgacgaggc agcaccacac cgattcattg cccatgtatc tctggacgca
    22201 ctgatcttct tgaaccgtgc ccgcggggca cgacaccggc gcttgaatgt cttggaaccg
    22261 caacacgccg gtccacgcac tgaggtcgct cgtcttcatg atgccgaagc cgtcagacgg
    22321 aattgtcggg ctcggatagt tctgcgtaca cgcccacacg gtgccagcgc tgtcgttgta
    22381 caggcacccg atatgcggcg cgcctgcgag cgctgtccag gtcggcgatg ccgcggtcga
    22441 acaatccgtc gatatccacg cgccgagctc acgcgtgccc gcgacgcacg tcgttgcatc
    22501 cgtgcgcacg aggaacgaga ggccgtagag cgaattccgc gtcaggatct ttgtccagct
    22561 ctgccctgca tccgtcgatc gatagatgct gtcgccgctg ccaccgctct cgaacgtcac
    22621 cttgacgtag agcgtggtgt ctgacgtcgg cgagatgccg accacgtcga ttgtcgagtt
    22681 cgctgacacc ggcgtgatgc cggtcatgtt catcgccgtg tagctcgtac cgccattggt
    22741 gctcgtgtag agcaaaaaga tcttggggtt cacaccatcg agccggtagc ccgtgaggta
    22801 gatacgctgc gcattgcccg gtgcgatcgc gagtgatgac caccagtcgc ccgcctgccc
    22861 tggattcgcc gacgtcggga acgtcatccc gtcgtcggtc gacttgtaga tcttcgcgtc
    22921 cgcgagatcg gctgcagcgt agtagatcgc accgtcgggc ccgcctttga cccgagccac
    22981 gaaggtcatg cccgggggcg ccgacgcgaa cgtgcatgcg ttgcggcgaa ccttgagccc
    23041 gtcgaacgtc gtcgcgaaga tcgcgcccgt cgacgaatac acatagtccg gatcgtactg
    23101 gccgccgtac ccgaccgcgc gctcgcacat ccattgccat gtcgcgccgc cgtcccgcga
    23161 ctcgacgaga ccgaacgtca tgccggcgag aatgtgctgg tcgttgtcgc gtcgaaaatt
    23221 gatcgtcgag gtacctggat cgcgaccatt tgcatacgcc gacgtcgcga acccgacggc
    23281 aagcaatcca gcccagacga aacgcatggg cccatactac cggtgctcgt cggtgcgcga
    23341 aatggcccga tcgagcacta catccgagcc ttttcgcggg cttcgcgcac acgaaacgga
    23401 gttgcaacaa ccgcgacggc acgggcgtcc attcatgtat gcgcgccgtc attgccgtcg
    23461 ccgtcgtcgg gttgctcgcg tgtgatcagg gcgtcgtggc caaaccgaag ccggcggtcg
    23521 cgacagtgac gccgcctgcg ccacccaacg cggagctgac tgcggatgga ccgttcaccg
    23581 ctgccgcgtg ggacctcgtc gcgccgctgc tgacgactca ccgcaacaag taacggccca
    23641 ccggcgcatt cgcgcaaagt gcggcgccgc accgacgagc cggccgtgta ctgccgatac
    23701 ggcaggatcc acgtcgccac gaggcggcgc cgcgcgcagc tcgcgtgatg cttcgaagcg
    23761 gtgcgctccg caacccccgc gatcacgacc gagcttgctg cgatcgcacg cgagatcacg
    23821 cacctccgcc ggcgcgtgct gctcctgcac gcagcggtcg tcaccggcgg cgtcgccgtg
    23881 ctcgtgcgcg catttcacgg cgtgagcctc accgagcgct cgcgcttctt cgcgtactgg
    23941 gccgcgattc tggtgatgtc gctcgtcgca ttcgcgatca gcacccgcat gcgcgagcgc
    24001 ctcgcgctgc tgtttgctcg tgcgcgcttc atcgagattc gcaaccgcta cggcggcgag
    24061 tagatgtaga gcgtgtcggt cggcaggtac gcgagcgccg gtgccccgcc gggcacgtag
    24121 aagcgagtgc caaccgctgc gccttgggtg cctgcgcgat tgaccggtaa cagctcgagg
    24181 ctggaccagc gatcgagcga cgcatcgtag aggtcggctc cacgcagtgc cgaattgccc
    24241 gattcgccgc cgacacagat cagctgatcg gcgagcgcgc cgtacgcgca accgccgcgc
    24301 ggtaccaaca gcgtggccgt gcgcggttcc cacgccgttc cgcccggcgg taatgcaagc
    24361 acgtcgctca gcggtttcgt cgaatcgatc gtgcgcaggc cagctgcgac aagcaacgtg
    24421 ccgtcgggcg tgcgcattgc cgccggatgc gagcgcggcc cgggtaacgc gggcagctgg
    24481 gtccagctgt cgtttgcgat gtcgtagtcg agacacgtgg cgagtgcatc ggtcgtcgac
    24541 gcgccaccga gcagatagat gtgcccgggc gccgagacga cgccagcggc gccgcgctcg
    24601 agacctgccg gcagtcgtgc gagcggtcgc catgcgctcg cgccggcatc gagtgcccac
    24661 gcatcgccgc gcgcgatgaa ctgttcgccc tcgagaccac cgagcaggaa caacgtggtt
    24721 ccgttgctcg cgagatcgat atgcgaccac tgcactggtg ctggaggcag cggcggcccc
    24781 cacgtctcgg tctcggtatc gaacacgtcg actgcatccg tgatgtgcag gccttccgat
    24841 acgccggtgt cgaagccacc gagcacgacg agttgcgtgc cgagcgccgt cacgcctggc
    24901 tcgagtcgac gcgcgggtat cttcgggccg agtcgccacg ccgatggtgc cgcgtcgccc
    24961 tgcatcgtcg tgtcgtcgcc gcagctagcg ccgaggacga gcacgagcca tgcggcgtga
    25021 cgcctcaccg ccggcgtgcc tcaccgcgtt ttacggtctc ggcgatcttc gcggcaaacg
    25081 ccggccacag cgcgcgcggc aggtcgtggc ccatgccctc gatcacgagc agctcggcac
    25141 cgggaatcgc cgcggccgtc gcacgtccac cgctgacgtc gcacagcttg tcgaccgcgc
    25201 cgtggatcac catcgtcggc acgtcgagcg agcgcaggcg agcggtccgg tctccggacg
    25261 ccagcaccgc aacgccctgg cgcatcacgc cgagcggatc gaacgagcga tcgaacgcgc
    25321 gcccggcgcg ctcgccgatc gcgtcgaggt cgacctcgaa tcccgtcgag ccgaccgccc
    25381 gaaacgcgtt ctggctgcgt ttgatcacgt cctcgcgcgt gaacgcgggt ggcgccgcga
    25441 acacctgcat gccctcgggg tgcggttgac cgacgctgcg atcgccagtc gacgacatga
    25501 tcgacgtcaa cgaacggacg cgcgtcggat gttcgatcgc gatcgtctgc gcgatgaagc
    25561 cgcccatcga ggcaccgaca aggtgtgcgc tgtcgatgcc gagggcatcg agtaacccga
    25621 cactgtcgcc cgccatatcc gagagcgagt acgacgccga cgacgtatcg cccgccattg
    25681 ctgcaatgaa gttcggcgtc ggcgcgctcg tcagatgggt cgactcgccg acgtcgcggt
    25741 tgtcgaaccg gatcacgtgt agcccgcggc cgacgatctc gtcgcagagg ccgtcgggcc
    25801 agccgatcag ctg
//
LOCUS       DASUFX010000449         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1877433, whole genome shotgun sequence.
ACCESSION   DASUFX010000449 DASUFX010000000
VERSION     DASUFX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877433"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFV99_18015"
     CDS             complement(<1..587)
                     /locus_tag="VFV99_18015"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX5061271.1"
                     /translation="MNALIAFIKLGRPIFLGGGFVLYALGAAIAHASGHPIDVGRYVF
                     GQAAVTAFQLMTHYANDYFDFEADIANHTPTKWSGGSRVLPNGLLPRRVALVAALGFA
                     ALGIALTAELDTRGVGSWTVPTFVLVLVLAWEYSAPPLRLCARGLGELATALVVTVLV
                     PWLGFYLQAPSMHGARMLALTIVPPALLQFAMLIA"
     gene            complement(584..1039)
                     /locus_tag="VFV99_18020"
     CDS             complement(584..1039)
                     /locus_tag="VFV99_18020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5061272.1"
                     /translation="MEDEIDLLDVQPLVDNELELHLVERTLAERERNRVPAYRFDLRV
                     AGEKAGTASLRVGTTQYLELYAGHIGYGVDLPFRGKRYAARTCLLLFGLARQHGMTTL
                     WITCNPENVASRRTCELVGGVLVDIVDLPPETDMYKEGERQKCRYRVRL"
     gene            complement(990..2141)
                     /locus_tag="VFV99_18025"
     CDS             complement(990..2141)
                     /locus_tag="VFV99_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061273.1"
                     /translation="MRVVAFAVLASFACSKHEEPAATRLPPSPPVRGAVGDTDLRVML
                     AEVASAKACEMMRGQLRGLRAQGNEPLTTGILWIRDCKITNDGTNVTFQLGGYGWQWN
                     EKREKKAGATFELRQYVKFAVDATIPGSLDIAYDRHDHVVSMWFSPTQTPKIEFTPTG
                     KVDVDAKGAWSSVIGGISSVFASSPDEQGRSKAEKLGTEQFENELARGLTIAIDLCTG
                     YQRFTIGRPQKGSLGPPNPGESTSRPIEIQPGGLFAFGAFEAPSGMHVIAHSDGPIRV
                     GVACADGVFPVVEAFAQDQAQPPIAMLAQQDIDGTGELAVKAQRCKVAVVVRSLAQRK
                     VTFDWQRPPREIAQSTGGSAIHCARKQTVFSDGDGGRDRPLGRAAARRQ"
     gene            complement(2192..2590)
                     /locus_tag="VFV99_18030"
     CDS             complement(2192..2590)
                     /locus_tag="VFV99_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061274.1"
                     /translation="MKLVALVAVVCVACVAHSRNPPPAAPAPGYATVSPGGIACPDGQ
                     TCDWSCDDGNCAYACVRGATCNVSCDGGNCRLDCAEGSTCNLSCDGGNCAAQCTSGST
                     CNLDCDGGSCRGACTPDATCAAQCDGGGCS"
     gene            complement(2642..>3488)
                     /locus_tag="VFV99_18035"
     CDS             complement(2642..>3488)
                     /locus_tag="VFV99_18035"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEX5061275.1"
                     /translation="LYMQYGYVPSSAGRSVSMTAEYSHDDYALAQLASALGHTAEHDT
                     LMARRLGWRMLYDPAVGFLRGRATDGSFPTASFDALSWLDEYAEANAWQSLFEPGIHD
                     PDGIAEVLGGREAAVAKLTTFFEKAETEQSNSDESTANFPRKYYWHGNEPDLNTPYLF
                     LQLGRPDLTYKWARWVMDTMYSDQPNGVAGNDDGGTLGAWFVLSSLGLYPIAGSDRWI
                     VAAPLFPKARVLLGGHELVIETHGSGMYVKSVELDGVPLDMLEITQDQLATASLLHFE
                     MSNEP"
BASE COUNT          602 a         1186 c         1123 g          577 t
ORIGIN      
        1 atcgcgatca acatcgcgaa ctgcagcagt gccggcggca cgatcgtcag cgcgagcatc
       61 cttgcaccgt gcatgctcgg cgcctgcagg tagaagccga gccacggcac gagcacggtg
      121 acgaccagtg cggtggcaag ctcaccgaga ccgcgcgcac acaaccgcag tggcggcgcg
      181 ctgtactccc atgcgagcac cagcacgagc acgaacgtcg gcaccgtcca gctgccaacg
      241 ccgcgcgtgt cgagctcggc cgtcaacgca atgccaagtg cagcaaaccc cagcgccgcg
      301 acgagcgcga cacgccgagg caacaggcca ttcggcaaca cacggctacc gcccgaccac
      361 ttcgttggcg tgtggttcgc gatgtcggct tcgaaatcaa agtagtcgtt cgcgtagtgc
      421 gtcatcagct ggaacgcggt cacggccgct tgaccgaaga cgtagcgccc gacgtcgatc
      481 ggatgcccgc tcgcgtgcgc gatcgcagcg ccaagcgcgt agaggacgaa gccgccgccg
      541 aggaagatcg gccgaccgag cttgatgaag gcgatgagtg cgttcatagc cgcacccggt
      601 aacgacactt ctgccgctcg ccttccttgt acatgtcggt ctccggtggc aggtcgacga
      661 tgtcgacaag cacgccaccg acgagctcgc acgtgcgccg cgacgcgacg ttctccgggt
      721 tacacgtgat ccacagcgtg gtcatgccgt gctgccgcgc gagcccgaac aacagcaggc
      781 acgtgcgcgc cgcgtagcgc ttgccgcgaa acggcaggtc gacaccgtag ccgatgtgcc
      841 cggcatacag ctcgaggtac tgcgtcgtgc cgacgcgcaa gctcgcggtg cctgccttct
      901 cgccggcgac gcgcaggtcg aagcgatacg ccggaacgcg attgcgttcg cgttcggcga
      961 gcgtgcgttc gacgaggtgg agctcgagct cattgtcgac gagcggctgc acgtccaaga
     1021 ggtcgatctc gtcctccatc gccgtcggag aataccgtct gtttgcgcgc gcagtgaatc
     1081 gcggagcccc cggtcgactg tgcgatttcg cgcggtgggc gctgccagtc aaacgtgacc
     1141 ttgcgctggg cgagtgaacg cacgacaacc gcgactttgc agcgctgcgc cttgacggcg
     1201 agctctccgg tgccatcgat gtcctgctgc gcgagcatcg cgatcggtgg ctgcgcctgg
     1261 tcctgcgcga aggcctcgac taccggaaac acgccatccg cgcacgcgac cccaacgcgg
     1321 atcggtccgt cgctatgcgc gatgacatgc atgccgctcg gcgcctcgaa cgcaccgaac
     1381 gcgaacaggc cgccgggctg gatctcgatc ggccggctcg tcgactcgcc gggattcggt
     1441 gggccgagcg agcccttctg tggccgaccg atggtgaagc gctgataacc ggtgcacaga
     1501 tcgatcgcga tcgtcagccc gcgggcgagc tcgttctcga attgctcggt gccgagcttc
     1561 tccgccttgg atcggccctg ctcgtcgggc gagctcgcga acacgctcga gatgccgccg
     1621 atcaccgacg accacgctcc tttggcgtcg acgtcgacct tgccggtcgg cgtgaattcg
     1681 atcttcggtg tctgcgtcgg cgagaaccac atcgacacga cgtgatcgtg gcggtcatac
     1741 gcgatatcga gcgagcctgg aatcgtcgcg tcgaccgcaa acttcacgta ctggcgcagc
     1801 tcgaacgtcg cgcctgcttt cttctcgcgc ttttcgttcc actgccagcc atagccgccg
     1861 agctgaaacg tcacgttggt gccgtcgttc gtgatcttgc agtcgcgaat ccacaagatg
     1921 ccggtcgtta gcggctcgtt gccttgcgcg cgcaacccgc gcagctggcc gcgcatcatc
     1981 tcgcatgcct tcgccgatgc gacctcggcg agcatcacgc gcagatcggt gtcaccgacg
     2041 gcgccacgca ccggcggcga cggaggcagt cgcgtcgctg cgggctcctc gtgcttgcta
     2101 cacgcgaaac tcgcaagaac cgcgaacgca accactcgca tgcacgtgtg cggtgcaagt
     2161 cgcgcacctc atgcggccgt gcgcctatgt ctcagctaca gccgccgccg tcgcattgcg
     2221 ccgcgcaagt cgcgtcgggc gtgcaggcac ctcggcaact tccgccatcg cagtcgagat
     2281 tgcatgtgct gccggacgtg cactgcgcgg cgcaattgcc accatcgcag gagaggttgc
     2341 acgtcgagcc ctcggcgcag tcgagccggc agttcccgcc atcgcagctg acgttgcagg
     2401 tcgcaccacg aacacacgcg tacgcacagt taccgtcgtc gcagctccag tcgcaggtct
     2461 gcccgtcggg gcatgcaatg ccacccggcg acaccgtcgc gtagccggga gcgggtgccg
     2521 caggcggcgg atttcgcgag tgcgcgacgc acgcgacaca gacaactgcg acgagggcaa
     2581 cgagtttcat aggtcctccg gggcaaggag ccgcgagatc gccgatcggt ttacgtggcg
     2641 atcacggttc gttgctcatt tcgaagtgaa ggagcgacgc cgtcgccagc tggtcctgag
     2701 tgatctcgag catgtcgagc gggaccccgt cgagctccac gctcttcaca tacatgcccg
     2761 agccgtgggt ctcgatcacg agctcgtggc cgccgagcag cacccgcgcc ttcgggaaca
     2821 gcggcgcggc gacgatccag cgatcgctgc ccgcgatcgg atagaggccg agcgagctga
     2881 gcacgaacca cgcgccgagc gtgccgccgt catcgttgcc ggcgacgccg tttggctgat
     2941 cggagtacat cgtgtccatc acccagcgcg cccacttgta cgtgaggtcg ggacggccga
     3001 gctggaggaa caagtacggc gtattgaggt cgggctcgtt gccgtgccag tagtacttgc
     3061 gcgggaagtt cgcggtcgac tcgtcactgt tcgattgctc ggtctccgcc ttctcgaaga
     3121 acgtcgtcag cttcgcgacc gccgcttcgc gaccaccgag cacctcggcg ataccgtccg
     3181 gatcgtgaat gcccggctcg aacagcgact gccacgcgtt cgcctccgcg tactcgtcaa
     3241 gccacgatag cgcgtcgaac gaagccgtcg ggaacgagcc gtcggtggcg cggccgcgca
     3301 agaagccgac cgccggatcg tagagcatcc gccagccgag tcgccgcgcc atcaacgtgt
     3361 cgtgctcggc ggtgtgcccc agcgcgctgg cgagctgcgc gagcgcgtag tcgtcgtgcg
     3421 aatactcggc ggtcatgctc accgagcgac cggcgctgct cggcacgtag ccgtactgca
     3481 tgtacagc
//
LOCUS       DASUFX010000450        19647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1045148, whole genome shotgun sequence.
ACCESSION   DASUFX010000450 DASUFX010000000
VERSION     DASUFX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19647
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045148"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="VFV99_18040"
     CDS             complement(<1..1776)
                     /locus_tag="VFV99_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061276.1"
                     /translation="MRCALLLCLLVACGGSQIQERKATDVSKLGPATLEATKPREGEP
                     RDAKVRVWVDAGVRATPNWKEEINDQIDYAGQLLTPLLGIRLKVEAIKDWDRTGDPHT
                     ALKDLAAADDGKDAIWVIGYVTPGDAASKAMPELGDAQLLGKHVVVRGWAEKPETDAL
                     AAGLPDLKAAERNEVLAAHRRHKQTVVLLHFLSRTLGAVAESDPAWIANPMYSPKQAS
                     FADRTRELMQTAIDRKLADDETPVIAKEILEKVSKEEWGGWISTDRDDVTKQLTAMVT
                     ADRMGQAAGEVPMAALEQFERIKVLARKNDMTNAMAELENLLVAYPGNATIYELKCEL
                     LLAKPGVKDKATRAACTRVSELAPTDPSPHIAVAAALVKERDIASARAELLTAAGKIE
                     QLKTGAAEQWKKLIGFYYGGLGSLTWTEEVIAKAKLENDPVVAEIASKRARYGVPRGA
                     KFVKPDDEGPLLGAVQDALGLVYASKYPQAEKAIAAGQKKWPGAPGFFAVRCDLELRQ
                     ARLDGAKAACAKALDLDPNESWALYLSAVIALKDTSPAGTKAGIEKLKKAIAVDAELA
                     QAWRTLAKAYARAKNQVALDQLRKDY"
     gene            1793..2218
                     /locus_tag="VFV99_18045"
     CDS             1793..2218
                     /locus_tag="VFV99_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061277.1"
                     /translation="MIAGRASRTIAAMTRLLLDERILGDGTHARTYATFVDGQVRVED
                     EQGAHGALSIAALDRVMVRYGLPLESSVALDGEALDLGEGRRLRRLRYHTPVDATGRD
                     YLVWERTGEAPLAVIATHATAALRYLVLRLDGEWGASKI"
     gene            2322..3416
                     /locus_tag="VFV99_18050"
     CDS             2322..3416
                     /locus_tag="VFV99_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061278.1"
                     /translation="MLRSVWLTCVLSVVSTTAWAQSAQPTTASAVATIPVAQAQQAAK
                     SAEQKLVTLTNQRVDLRRRYDEQLSAIDRLKKQKASWRRDRELNKAQADANDTAKRLT
                     AIDNQIKLAQQDVGKARNVMVGAIDAELATATGARADQLKKLRAQISPALPTPKKIVI
                     PDAEIDPLSDPEELERQASALAAVEKQLEAQRKGLDQQHKDLTLVAELRSAHERAGEL
                     STRDDDQPHRGAQKPTSRSAADEASPTAGAGNGDSAGGSTGGAGGGSGSGAQSGGTGG
                     TTDQSIGGETFGGDKHTSSTFESNAAVALGEVIDKSTIEGMLRASRSGNPKDRAEAAK
                     LARDAVAKQIDQLKKKRALIELRAKQLRKH"
     gene            complement(3413..4042)
                     /locus_tag="VFV99_18055"
     CDS             complement(3413..4042)
                     /locus_tag="VFV99_18055"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5061279.1"
                     /translation="MTSKTLAALLLVASSTTAAFADDYELGARVGGYGFRRQDADGQW
                     DVCRMNGFGVFGGRALRGPLFVEAGIDMYSSIGAAQPTDLPIDRTSGLFSGAIGARTQ
                     LATWLRGYVQLGGGVELTRVSVPYGDDHIRDEKAMPEGFFGLGFDIKLAKHTLVGSSF
                     RTLLMGNFDYDPANLEMKDQWAWKPTPDVVFDASPDVATQVQFYVRHEL"
     gene            complement(4176..5369)
                     /locus_tag="VFV99_18060"
     CDS             complement(4176..5369)
                     /locus_tag="VFV99_18060"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5061280.1"
                     /translation="MKKILFVSVLGLVSSTAYAGGLAVGEQNAVSSATGGAGAGRDDD
                     AGAAWHAPAALADDGGLRLGLSLALARPSLEARGDDWNASTESKWSTPPHLDASFAKD
                     RWAAGIAIGVPFGGGVTWPTTWPGATQAVRTELMVIRFAPFGAFRVGPLRVAAGAHFD
                     AGRLQIQRNLDFIDMQGDVRLDLAGRGFGVDASAYYVPREDVGIGLTYRSRTTIDFDG
                     NANFTAPDAFSEKTPDQTAKTSMTMPDQLVLGARWRRGPLTALADLEYTRWSVNEKTV
                     VDFTNDATPNAIQENGWHNTVAVRTGAEWRHTPKLTVRGGAYYDPSPVPAEHLTPSSP
                     DSNRVAATAGASYRFSPAWTADVFGEHMWILRRDTTSVDTMPASYGGTAIVLGAGVRW
                     TPAAK"
     gene            complement(5366..7861)
                     /locus_tag="VFV99_18065"
     CDS             complement(5366..7861)
                     /locus_tag="VFV99_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061281.1"
                     /translation="MRKLAFIGLFLAGCTTLELDPAPKIIHARFDPDASVIPMPTDVL
                     RDAAAGRLNLPNDTEKERAKLNPTEAEFYDYLETLDGWSSLMSATVEFTGAIDATSIT
                     DGTVQIWHWGPVPQRVNDARISLSQDGKKLTIDPPRTGWLRGDRYVALVRGGSQGLKG
                     QAGEKVECDAAFYFLRQTEALDTPNHEHAFPGDDHGERVSNASRLEGIREDLLGPFDF
                     FADQQLPREDVAALWAFTVTKRTELAMDQPSQRIPLPIDLMIEPSTGLVDVPAAPWDS
                     AVEKEAKGRLAELDGASLSGSQLFEFTAPLNPTTINEANVQLYQIGGNATLVPATVEL
                     LTDKQHIQVTPKAGRLAEQTRYALVLSKDVQDALGKQPAIMPIGHFLRSHTPVLVDGK
                     SQIHAVGDHDAIKLENSRQELAAALDTIGRDKILAAWPFTTMSVKEPLAALRQLPAKL
                     GTSTEPANVTTMTPTQALGDFVFGIGSILNVKTVYNGTIETANFLDPKLRSWRGDGGH
                     AVENVKFTMTLPKTGTGPVPVVIFGHGLITERRFVLAVGDALAAKGYAAISIDFPYHG
                     SRTYCAKGGPISVVDPTDGSLTSLEPCQSGTTCNDEGRCVDANGNGNKLATFGVLDMP
                     VASGAMFLEIEHIANTKDHFQQALVDLGALDRVLRLGNWQSVIGRPIDTSKIFYAGQS
                     LGGIMGAVFLGTDPDIKRAVLNVPGADLVPMFDDSTFFSAQLDAFFTRQKVTRESFEG
                     HRFVMVAKWFMDKVDPLHYGEKITENRAVLLQMATLDMIIPNNNTKELEAVTHAPRRD
                     YIAEHAFLTIPIEPEYLRGTGDLANFLSGELQP"
     gene            8016..8657
                     /locus_tag="VFV99_18070"
     CDS             8016..8657
                     /locus_tag="VFV99_18070"
                     /inference="COORDINATES: protein motif:HMM:NF037470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HEX5061282.1"
                     /translation="MRSTPLSRLLDEPEPAKRVTPLDAFKLARTKWLAGERIDVGRIA
                     SELGVGRATVFRWVGTRENLYGEICSALFKKELDRAVAGARGSGSDYLVDVMTKLLHS
                     LAAATPLRRFVAEDPEFALRVLTSRHSPVQRRCTIAVKELIDEATEAGEMQPALPSDE
                     LAYIIVRITESFLYRDVITGVSADIEAAIAAIRILLTAEPDPSRPRKRSKLRH"
     gene            complement(8654..9088)
                     /locus_tag="VFV99_18075"
     CDS             complement(8654..9088)
                     /locus_tag="VFV99_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061283.1"
                     /translation="MRRLRIAIGVAVALGVLTIVLGYIPVSITDMDYQRHLERAGSDE
                     LTLLVVMMMLPVGWAYRRPKWPLLVMWIMWLTTWLMLGAILFIADTDQAYEMVDPFWM
                     RPLLVVTTIAMALVVDVVLPVLRGRNKSPPPSTLPNARVVDR"
     gene            complement(9108..10232)
                     /locus_tag="VFV99_18080"
     CDS             complement(9108..10232)
                     /locus_tag="VFV99_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061284.1"
                     /translation="MRRVAVLIALAAAARGAEANPWLVTGEAGAELDSNVQRVETGPG
                     LDTEEIAAWVMRFGARVNRKGKVLGGAFALDLSDLTRVVANDDVAVENVAVLASNLRW
                     LHKFGDRPVLGGFSLTAIDAIPLSDEIGARTFVNLGADAMLAMRNDDEHELTLALGVH
                     DFTYKPDHDFDYRGPAANARLELMLWQPPAKTKSLELVASLGFENRAFDAVALINACP
                     PGTKADPMCTAATDIERRDRYQRLGIELTWVGRQVAALGYQITIIDSNSFGQSLVRHR
                     VQASTTVALPNKVYGTLLAILQADQYLDGLLVRRDLQQQEFTNVEDENRSSLQLRLAR
                     VLVGSWAVEGRVAVWRNILGNTMELDFKRELVYAGLVYGK"
     gene            10275..10595
                     /locus_tag="VFV99_18085"
     CDS             10275..10595
                     /locus_tag="VFV99_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061285.1"
                     /translation="MRWLAALFFVFMTGCQACKGDPEKCDRACRNYAQQVFWEGANAE
                     IDKLPADQRDAARKQKMAEFAANLEKGVDLCVSKCVSANNTKDTDCLIGAKSAAAAKA
                     CVKD"
     gene            complement(10633..11865)
                     /locus_tag="VFV99_18090"
     CDS             complement(10633..11865)
                     /locus_tag="VFV99_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061286.1"
                     /translation="MRLGYLSMLALVTACGGHLKRETIGSGATAVQARGSTDPAPAGS
                     INLPRGVYELKLRFDVPRAQLVEWTVICPGAAQSGVAGETTEEYRARRIAELRAEKQR
                     ERERVAGITGAVIGAVTPDVHAHAQTNHATVDATVSGQAVGNAAGVAIANATVSDAVE
                     LAPGDVGAGRLKAKASVITLDSGTCTVSAIADDANVLASYEVVRVRDLDAEARARREA
                     DPKVQARIAIQMQLRVKALDIRGQLIRYFTGECHARADHREYLEEQPLVVRAAMREYL
                     IALGAIERPPMPAPKPEVPGDAPYDGAEWVAGEWIWTGIEWQWEVGVWRDASVATVRD
                     HRTSDTSDRDHRSDPLPTVRDHRDETVSRDHRSEPVVRDHRDEPKPHSTPVVRDHRKD
                     DDDKKKDDDAPKVRDHRH"
     gene            12041..12355
                     /locus_tag="VFV99_18095"
     CDS             12041..12355
                     /locus_tag="VFV99_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061287.1"
                     /translation="MVRSLRLWRLWIAITLAACGGGHRGDTYAKASHEQEQCCESLSG
                     PSRDECLQKLVRVDDPEVAKTRANQDQYACVVEHFVCDPTSGHATQASAQAQLDCIQD
                     LR"
     gene            12398..13384
                     /locus_tag="VFV99_18100"
     CDS             12398..13384
                     /locus_tag="VFV99_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061288.1"
                     /translation="MQRGSLLLVMAAACWQKPSATTGSVTSSVMARPQPRSGLACAFV
                     SARDSENHWSNAQIYLVDGVHRIPVNLTNDAQTNWAPAISPDGREMVFVASRGHGLFV
                     LELETDESREVPIATPGQPASPRWLSGHRIAYAAFTDRTAVWLVDMRGDSPRQLTSPG
                     PKESDDGGFAMIDGGKRIVFSRYDRLTGERHLYVANTDGSDSPIRITNTSKVSETLPT
                     VSHEGQLLAFRRFAHTDFSDQIRIVSTRDFSLVRDISLASPAKWNINGLDFTGDDRQI
                     VFGADADDVGGNLQNLHGELFVVGVDGGNQRRLTKNAAYDGQPACMPAVISK"
     gene            13387..13614
                     /locus_tag="VFV99_18105"
     CDS             13387..13614
                     /locus_tag="VFV99_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061289.1"
                     /translation="MPVNRCPHCGLVNPAKTMTCDCGYSFVDGAPPRFSHDRPRARNS
                     SAGSVFATRVMIRIALAVLFAGFAFAIRACG"
     gene            complement(13619..15955)
                     /locus_tag="VFV99_18110"
     CDS             complement(13619..15955)
                     /locus_tag="VFV99_18110"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5061290.1"
                     /translation="MAAAWAAWAAWTSRPRSFARMRPARRGVFIFGAVSCAGMRALIA
                     ALVVLLASPARADAPLPDTPTRLMTLGRVWAEAKFFHPTLQTKDIDWDAALVAAIPKV
                     EAAKTQKELDAAVQSMLAVIGDPVTRVLDDKPVDIAAPADWLVTANHVTEVKLSVFAA
                     GTLDFKGSREKATQIATEAAGAKVMIIDLRNVTARAWVSRWIVEQFASVLPAIDEWPL
                     MRSVEHRGWRTQQGTTSGNYFSTFIVTGGRPPKPAPKQGPAHVVFVADPTAGLPMEAL
                     ALQATGKATIVAQGALRDDAIVDTATIELGGGSRAQVRLNELLSGPPTADISVPAGQD
                     PMVRARQLAKTLAKKLPAAKRTRPKPRELPQLRVRDDDDYASTPLPSREHRLLAGIRM
                     WAVIDRFEPYRYLIKDWDGVLREMLPRLDAARDRETYLRVLKEMAARVDDGHVNVFPG
                     APDPTAKPKGIAPFEVRLVENRPTIVRLLDAAATAKLGVAVGDVIEMIDGKPAAETFA
                     ARRAITSGSTTESRDQRAAAYMLTGDDGTQVKLGVRGARGKLRDVVVTRAKSYAAAFD
                     APSSEPHWKKLAGNIGYIDLRTLTVPEVPAAMKDLDGTKAIVFDMRGYPNGVAWSLAP
                     RLNTRGAKYGAQFLAPHVFGMLADDEVADTRTRFLQKLPPLEPGTTIYRGKVIVVIDD
                     RAISQSEHTCLFLQEAAGATFVGSPTHGANGDVTVMRLPGGLRMSFTGQEVRHVDGKQ
                     LQQVGITPHIVIRPTLAGVRAGKDEVLDRAIELAIKGR"
     gene            complement(15915..17555)
                     /gene="groL"
                     /locus_tag="VFV99_18115"
     CDS             complement(15915..17555)
                     /gene="groL"
                     /locus_tag="VFV99_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5061291.1"
                     /translation="MAAKDIIFDEKARAKVQIGVDTLANAVKVTLGPRGRNVVIEKSW
                     GAPTVTKDGVTVAKEIELENKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQAIYRE
                     GSKLVAAGHNPMELKRGIDTAVAAIVESLKKLTTPTKGKEDIAQVGTISANGDTEIGE
                     MIAEAMKKVGKEGVITVEEAKTLNSELDVVEGMQFDRGYLSPYFVTDTERMECVLNDA
                     YILIHEKKISNMKELLPILEQVAKQGKPLLIIAEDVDGEALATLVVNKLRGTLQICAV
                     KAPGFGDRRKEMLKDIAVLTGGQAVTEDLGLKLENITLADLGSAKRLTVDKDNTTIVD
                     GAGKKNDIEARVKQIRAAVEETTSDYDREKLQERLAKLVGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALIRAQSALDKVKLSEEQAFGVSIVRRAIEEPL
                     RQIAMNAGVDGSIVVSKVKEAQGAFGFNAQTLEYTDLVKAGVIDPTKVVRTALQNAAS
                     VSALLLTTEAMIAERPKKEAPAPAPGGGGMGGMGGMDF"
     gene            complement(17567..17857)
                     /gene="groES"
                     /locus_tag="VFV99_18120"
     CDS             complement(17567..17857)
                     /gene="groES"
                     /locus_tag="VFV99_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5061292.1"
                     /translation="MNVRPLQDRILVRRVEEAEKTRGGIIIPDSAKERPLEGKVIAVG
                     SGKRLEDGTLVKLDVKAGDRVLFGKYAGTEIKVDGVEHIIIREDEVLGVIES"
     gene            18064..18624
                     /locus_tag="VFV99_18125"
     CDS             18064..18624
                     /locus_tag="VFV99_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061293.1"
                     /translation="MDVTLAASVDEFFHEVVTDALAVVDLDASEPASWYLVGLLGEMT
                     RARLTDEPLGIKLAQPTADAGERVRTLKHVGDTSLYVAGFFAESLTRSLVDVDYYVGL
                     GQNAYAQLARTFGGQKSITEVYAELADKFPKFVDVLTEVRKRTDFATPDVGRLYEIWL
                     KTRDEWIEKKLRALGVLVDVDKKVIQ"
     gene            18624..19262
                     /locus_tag="VFV99_18130"
     CDS             18624..19262
                     /locus_tag="VFV99_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061294.1"
                     /translation="MRTLARVQRGLEALYRVDTGVTVDDFMIDEPTRDGLVPTRRPRE
                     QLLVCEEAGEMSLALFIDPAALSNLAQHDPGNRLGDHNLGDFLLAVEGVSHFIYAIWC
                     ARAERPVTQLELELQAEVDKYVTCLLTTEAAPSQSEVLRYRLFVDAEYEPDLDGDERD
                     RYRAANDNAHRYAAWLESTFVATRRIPEMLGELRRFYREGLAAKLARIARAA"
     gene            19313..>19647
                     /locus_tag="VFV99_18135"
     CDS             19313..>19647
                     /locus_tag="VFV99_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061295.1"
                     /translation="MLVCAKTDVGRARTTNEDACSVTELASGQVIDADSADRTVDVGH
                     KGVLLALSDGMGGHQAGEVASALVLESLGSELTQDAKGPVHRQLEDAVHRANRAVLEA
                     AKSADRHGMG"
BASE COUNT         3171 a         6497 c         6480 g         3499 t
ORIGIN      
        1 gtagtccttg cgcagctgat ccagcgccac ctgatttttt gcccgcgcgt acgccttcgc
       61 gagcgtgcgc cacgcttggg cgagctcggc gtcgacggcg attgccttct tcagcttctc
      121 gatgcccgcc ttcgtgccgg ccggactcgt gtccttgagc gcgatcacgg ccgacaggta
      181 cagcgcccac gactcgttcg gatcgaggtc gagcgccttg gcgcacgctg ctttcgcgcc
      241 atcgagccgc gcctggcgca actcgagatc acagcgcacc gcgaagaacc ccggcgcacc
      301 cggccacttc ttctggccgg ccgcgatcgc cttctccgct tgcggatact tgctcgcgta
      361 gacgaggccg agtgcgtcct gcacggcgcc gagcagcggc ccttcatcgt ccggcttcac
      421 gaacttcgct ccgcgcggca cgccatagcg cgcgcgcttc gacgcgatct cagcgacgac
      481 cggatcgttc tcgagctttg ccttcgcgat cacttcctcg gtccacgtca acgagccgag
      541 gcctccgtag tagaagccga tcagtttctt ccattgctcg gcggcgccgg tcttgagctg
      601 ttcgatcttg ccggccgcgg ttagcagctc ggcgcgcgcg ctcgcgatgt cgcgctcctt
      661 cacgagtgcc gcagcaaccg cgatgtgcgg cgacggatcg gtcggcgcca gctcgctgac
      721 gcgcgtgcat gctgcacgtg tcgccttgtc cttgacgccc ggcttcgcga gcagcagctc
      781 gcacttgagc tcgtagatcg tcgcgttgcc ggggtacgcg acgagcaagt tctcgagctc
      841 ggccatcgcg ttcgtcatgt cgttcttgcg cgcgagcacc ttgatgcgct cgaactgctc
      901 gagcgcggcc atcggcacct cgccggcggc ttgtcccatg cgatcggcgg tgaccatcgc
      961 agtgagctgc ttcgtgacgt cgtcgcggtc ggtcgagatc cagccgcccc attcctcctt
     1021 cgagaccttc tcgaggatct ccttcgcgat caccggcgtc tcgtcgtcgg cgagcttgcg
     1081 atcgatcgcg gtctgcatca gctcgcgcgt gcgatcggca aacgatgctt gcttcggcga
     1141 gtacatcggg ttcgcgatcc acgccggatc ggattcggcg acggcgccga gcgtgcgcga
     1201 caggaaatgc agcagcacga cggtctgctt gtggcggcga tgcgcggcga gcacttcgtt
     1261 ccgctcggca gctttcaaat cgggcagccc tgcggcgagc gcgtcggtct ccggcttctc
     1321 tgcccagcca cgcacgacga cgtgcttgcc gagcagctgc gcgtcgccga gctcgggcat
     1381 cgctttgctc gcagcatcgc ccggtgtcac gtagccgatc acccagatcg cgtccttgcc
     1441 atcgtcggcg gccgcgagat ccttcagtgc ggtgtgcgga tcaccggtgc gatcccaatc
     1501 cttgatcgcc tcgaccttga gccgaatgcc gagcagcggt gtcagtagct gaccggcgta
     1561 gtcgatctgg tcgttgatct cttctttcca gttcggcgtc gcccgcacgc cggcgtcgac
     1621 ccagacccgc accttcgcgt cgcgcggctc gccttcgcga ggcttcgtcg cttcgagcgt
     1681 tgccggcccg agcttggaga catcggtggc cttgcgttct tggatctgac ttccgccgca
     1741 ggcgacgagc aaacacaaca gcagcgcgca gcgcatccga agactttccc cgttgattgc
     1801 cggtcgcgca agccgcacca tcgccgcgat gacacggctc ctgctcgacg agcggattct
     1861 cggtgatggc actcacgccc gcacctacgc gaccttcgta gatggtcagg ttcgcgtcga
     1921 agacgagcag ggcgcgcacg gcgcactgtc gattgctgcg ctcgatcggg tgatggtgcg
     1981 gtacggactc ccgctcgaga gtagcgttgc gctcgacggc gaggcgctcg atctcggcga
     2041 aggacggcgg ctccgcaggc tgcggtacca cacgccagtc gatgcgacag gccgcgatta
     2101 cctggtctgg gaacgaaccg gcgaggcccc gctcgcggtg atcgccaccc acgcaaccgc
     2161 cgccctccgc tacttggtgc tccggctcga cggagaatgg ggggcctcga aaatctgagg
     2221 gggaccgctg ccaaggattc acgatccggg ggcaaaacct caggtgaagt cagcgatgcc
     2281 gcatagttgg acgtggcata gcggctgcta tcgtataggc cgtgctgcgg agtgtctggc
     2341 tgacgtgcgt cctgtcggtg gtttcgacca ccgcgtgggc gcagtctgcc cagccgacga
     2401 cggcatccgc ggtcgcgacg attccggtcg cgcaggcgca gcaggctgcg aagtctgctg
     2461 agcagaagct cgtgacgctg acgaatcagc gcgtcgacct gcgccgccgc tacgacgagc
     2521 agctgtcggc gatcgatcgc ctgaagaagc agaaggcatc gtggcgccgc gatcgcgagc
     2581 tcaacaaggc acaggccgat gcgaacgaca ccgcgaagcg gctgaccgcg atcgataacc
     2641 agatcaagct cgcgcagcag gacgtcggca aggcgcgcaa cgtgatggtt ggtgcgatcg
     2701 atgccgagct tgcaaccgcg acgggcgctc gcgcggacca gctgaagaag ctgcgcgcac
     2761 agatttcgcc agcactgccg acgccgaaga agatcgtgat tccggatgcc gagatcgatc
     2821 cgctctcgga tcccgaggag ctcgagcggc aggcgtccgc gctcgcggcg gtcgagaagc
     2881 agctcgaagc gcagcgcaag ggactcgatc agcagcacaa ggacctcacg ctggttgccg
     2941 agctgcgcag cgctcacgag cgcgccggcg agctgtcgac gcgcgatgac gatcagccgc
     3001 atcgcggcgc gcagaagccg acgtcgcgct ctgcggccga cgaggcatcg ccgacggcgg
     3061 gggcaggcaa cggcgacagt gcgggtggta gcaccggcgg ggccggtggc ggtagtggca
     3121 gcggtgcgca gagcggcggc acgggcggca cgaccgatca gtcgatcggc ggcgagacgt
     3181 ttggcggcga caagcacacg tcgtcgacgt tcgagtcgaa tgccgcggtg gcgctcggcg
     3241 aggtgatcga caagtcgacg atcgaaggca tgctgcgtgc gtcgcgctcg ggcaatccca
     3301 aggatcgcgc cgaggctgcc aagctcgctc gcgatgcggt ggcgaagcag atcgatcagc
     3361 tcaagaagaa gcgcgcgctc atcgagctac gcgcgaagca gctgcgcaag cactagagct
     3421 cgtggcgcac gtagaactgg acctgcgtcg cgacgtcggg tgacgcgtcg aacacgacgt
     3481 cgggcgtcgg cttccaggcc cactggtcct tcatctcgag gttggcgggg tcgtagtcga
     3541 agttgcccat gagcagcgtg cggaagctcg acccgaccag cgtgtgtttc gcgagcttga
     3601 tgtcgaagcc gaggccgaag aagccctcgg gcatcgcctt ctcgtcgcga atgtggtcgt
     3661 cgccgtaggg caccgagacg cgcgtcagct cgacgccacc gccgagctgg acgtagccgc
     3721 gtagccacgt cgcgagctgg gtacgcgcgc cgatcgcacc gctgaatagc ccgctcgtac
     3781 gatcgatcgg caagtccgtc ggctgcgccg cgccgatcga gctgtacatg tcgatgccgg
     3841 cctcgacgaa cagcggccca cgcagcgcgc gcccgccgaa cacaccgaag ccgttcatcc
     3901 gacacacgtc ccattgcccg tcggcgtctt gccggcggaa gccatagcca ccgacccggg
     3961 cgccgagctc gtagtcatcg gcgaacgcag cggtggtgga agacgcgacg aggaggaggg
     4021 cagcgagggt tttcgacgtc atgcccccgg ctacatcaag aaccgcgccg tgaacgcgcg
     4081 ttctcagctc gtgaacccgg gactccaaac ggggacggtt tcaacgtttg aaacgtcccc
     4141 ggggctcgga gtcgccggat tcgacgacgg tcccgctact tcgctgccgg agtccaccgc
     4201 acgccggcgc cgaggacaat cgccgtgccg ccgtagctgg ccggcatggt gtcgacgctc
     4261 gtcgtgtcgc ggcggaggat ccacatgtgc tcgccgaaga catcggcggt ccacgcgggc
     4321 gagaaccgat agctcgcacc tgcggtggcc gcgacacgat tcgagtcagg cgacgacggg
     4381 gtcaggtgct cggcggggac cggcgacgga tcgtagtagg cgccgccccg aaccgtgagc
     4441 ttcggcgtgt gccgccactc ggcgcccgtg cgcacggcga ccgtgttgtg ccagccgttc
     4501 tcctggatcg cgtttggcgt cgcgtcgttc gtgaagtcga cgacggtctt ctcgttgacg
     4561 ctccagcgcg tgtactcgag atcggcgagc gcggtgagcg gaccgcgccg ccagcgcgcg
     4621 ccgagcacga gctggtccgg cattgtcatc gacgtcttcg cggtctgatc cggcgtcttc
     4681 tcgctgaacg cgtcgggcgc ggtgaagttc gcgttgccgt cgaagtcgat cgtcgtgcgg
     4741 ctgcggtacg tgaggccgat gccgacgtct tcgcgcggca cgtagtacgc cgacgcgtcg
     4801 acgccgaagc cacgaccggc gaggtcgagg cgaacgtcgc cctgcatgtc gatgaagtcg
     4861 aggttgcgct ggatctgcaa tcggccggcg tcgaagtgtg cgccggcggc gacgcgcagc
     4921 gggccgacgc ggaacgcacc gaacggcgcg aagcggatca ccatcagctc ggtgcgcacc
     4981 gcttgggtcg cgccgggcca cgtggtcggc cacgtcacgc cgccgccaaa cggcacgccg
     5041 atcgcgatgc cggcggccca gcgatccttc gcgaagctcg cgtcgaggtg gggcggggta
     5101 gaccacttgc tctcggtgct cgcgttccag tcgtcaccgc gcgcttcgag cgacggccgc
     5161 gcgagcgcga gcgagagacc gaggcggagg ccaccgtcgt cggcgagcgc tgcgggtgca
     5221 tgccatgccg cacctgcgtc gtcgtcgcga ccggcaccgg caccaccggt cgccgaggac
     5281 accgcgttct gctcgccgac ggcgagaccg cctgcgtatg cagtggaaga aacgagcccg
     5341 agaacggaga caaagaggat tttcttcatg gctgaagctc cccggagagg aagtttgcga
     5401 ggtcgcccgt accgcgtagg tattcgggct cgatcggtat ggtcaggaac gcgtgctcgg
     5461 cgatgtagtc gcggcgcggc gcgtgcgtga cggcttcgag ctccttcgtg ttgttgttcg
     5521 gaatgatcat gtcgagtgtc gccatctgaa gcagcacggc acggttctcg gtgatctttt
     5581 cgccgtagtg gagcggatcg actttgtcca tgaaccactt cgcgaccatg acgaagcggt
     5641 ggccctcgaa cgattcgcga gtgactttct gacgggtgaa gaatgcgtcg agctgcgcgc
     5701 tgaagaatgt cgagtcatcg aacatcggca ccaggtccgc gccgggcacg ttgagcaccg
     5761 cgcgcttgat gtcggggtcc gtgccgagga agaccgcgcc catgatgccg ccgagcgact
     5821 ggcccgcgta aaagatcttc gacgtgtcga tcgggcggcc gatcacggac tgccagttgc
     5881 cgaggcgcag cacgcgatcg agtgcgccca gatcgacgag cgcctgctgg aagtgatcct
     5941 tcgtgtttgc gatgtgctcg atctcgagga acatcgcgcc cgatgcgacc ggcatgtcga
     6001 gcacgccaaa cgtcgcgagc ttgttgccgt tgccgttcgc atcgacgcag cggccttcat
     6061 cgttgcacgt cgtgccggac tgacagggtt cgagtgacgt caggctgccg tccgtcggat
     6121 cgacgaccga gatcgggccg cccttcgcac agtaggtgcg tgagccgtgg tacgggaagt
     6181 cgatcgagat cgccgcgtag cccttggcgg cgagcgcgtc accgaccgcg agcacgaagc
     6241 ggcgctcggt gatgagaccg tgcccgaaga tcacgaccgg caccggaccg gtgcctgttt
     6301 tcggcagtgt catcgtgaac ttcacgttct cgaccgcgtg gccaccgtcg ccgcgccagc
     6361 tgcgcagctt cggatcgagg aagttcgcgg tctcgatcgt gccgttgtag acggttttga
     6421 cattcaagat cgaaccgatg ccgaacacga agtcgccgag tgcctgtgtt ggcgtcatcg
     6481 tcgtgacgtt cgccggctcc gtcgacgtac cgagcttggc gggcagctga cggagcgcgg
     6541 cgagcggctc cttcacgctc atcgtcgtga acggccacgc tgctaggatc ttgtcgcggc
     6601 cgatcgtgtc gagcgcggcc gccagctcct ggcgcgagtt ctcgagcttg atcgcatcgt
     6661 gatcgccgac cgcgtggatc tggctcttgc cgtcgacgag caccggcgtg tggctgcgca
     6721 ggaagtgacc gatcggcatg atcgccggct gcttgccgag cgcgtcctgc acgtccttcg
     6781 agagaacgag cgcgtaccgc gtctgctcgg cgagccggcc cgccttcggc gtaacctgaa
     6841 tgtgctgctt gtcggtgagc agctcgacgg ttgccggcac cagcgtcgca ttgccgccga
     6901 tctgatagag ctggacgttc gcttcgttga tcgtcgtcgg gttgagcggt gcggtgaatt
     6961 cgaacagctg gctgcccgac agcgatgcgc cgtcgagctc ggcgagccgg cccttcgctt
     7021 ctttctcgac cgcgctgtcc cacggtgcag cgggcacatc gacgagcccg gtgctcggct
     7081 cgatcatcag atcgatcggc agcgggatgc gctgcgacgg ttgatccatc gcgagctcgg
     7141 tgcgcttggt gaccgtgaaa gcccacagcg ctgcgacgtc ctcgcgcggc agctgctgat
     7201 cggcgaagaa gtcgaacggg ccgagcaggt cctcgcgaat gccttcgagg cgcgacgcgt
     7261 tcgagacgcg ctcgccgtga tcgtcgcccg ggaacgcatg ctcgtggttc ggcgtgtcga
     7321 gcgcttcggt ctggcgcaag aagtagaacg cggcgtcgca ttcgaccttc tcgccggcct
     7381 gacccttgag gccttgcgaa ccgccgcgga cgagcgcgac gtagcgatcg ccccgtagcc
     7441 agccggtgcg cggaggatcg atcgtgagct tcttgccgtc ttgcgagagc gagatgcgcg
     7501 cgtcgttgac gcgctgtgga accggacccc agtgccagat ctggacggtg ccatcggtga
     7561 tcgacgtcgc gtcgatcgcg ccggtgaact cgaccgtcgc cgacatcagg ctcgaccaac
     7621 cgtcgagcgt ctcgaggtag tcgtagaact ccgcttccgt cggattgagc ttcgcgcgtt
     7681 ctttctcggt gtcgttcggt agattcagcc gaccggccgc tgcgtcgcgc agcacgtcgg
     7741 tcggcatcgg aatcaccgac gcatcgggat cgaaacgcgc gtggataatc ttcggcgcgg
     7801 gatccagctc gagcgttgta cagccggcga gaaacagtcc gatgaatgcg agtttccgca
     7861 cgaatctccc tcccaggaaa tgtcggagat ggttctgcgc ctatattgaa atcgagtcaa
     7921 gaggcaattg agtttcggtc aatgaccaga actgccattc gatgcattgt gagtcaccct
     7981 tgacaaatga ctcatctaca ctgaggctgt tagaaatgcg gtccacacca ctttcccgcc
     8041 tgctcgatga acccgagccg gcgaaacgcg tgacgccgct cgatgcgttc aagctcgcgc
     8101 gcacgaagtg gctcgccgga gaacgcattg atgtcggcag gatcgcgagc gagctcggcg
     8161 tcggccgcgc gaccgtgttt cgctgggtcg ggacgcgtga aaacctgtac ggcgagatct
     8221 gttcggcgct gttcaagaag gagctcgacc gtgctgtggc gggagcgcgc ggcagcggca
     8281 gcgactacct ggtcgacgtg atgacgaagt tgttacattc actagcggca gccaccccgc
     8341 tccggcgctt cgtggccgag gacccggagt tcgcgctgcg cgtgctgacg tcgcggcaca
     8401 gcccggtcca gcgccgctgc acgatcgcgg tcaaagagct catcgacgag gcgaccgagg
     8461 cgggcgagat gcagccggcg ctgccgagcg acgagctcgc gtacatcatc gtgcgcatca
     8521 ccgagtcgtt cctctaccgc gacgtgatca ccggcgtgtc agccgatatc gaggcggcga
     8581 tcgccgcgat tcgcatcttg ctcaccgccg agccggatcc gagtcggccg cgcaagcgca
     8641 gcaagctacg ccactaacga tcgacgacgc gcgcgttcgg tagtgtcgac ggtggtggac
     8701 tcttgttccg cccgcggagg acgggcagca cgacatcgac gacgagcgcc atcgcgatcg
     8761 tcgtgaccac gagcagcggt cgcatccaga acgggtcgac catctcgtac gcctggtcgg
     8821 tatccgcgat gaacaggatc gcgcccagca tgagccatgt cgtcagccac atgatccaca
     8881 tcacgagcag tggccacttc gggcggcgat acgcccagcc caccggaagc atcatcatca
     8941 cgacgagcag cgtgagctcg tcgctgccgg cgcgctccag atgccgttgg taatccatgt
     9001 cggtgatcga taccgggatg tagccgagca cgatcgtgag gacgccgagc gcgaccgcaa
     9061 caccgattgc gatgcgcagg cgccgcacga tcattgcttc tcgtggatca ctttccatag
     9121 accagccccg cgtagacgag ctcgcgcttg aagtcgagct ccatcgtgtt gccaaggatg
     9181 ttgcgccaga ccgcgacgcg accctcgacg gcccagctgc cgacgagcac gcgcgcgagc
     9241 cggagctgca gcgagctgcg gttctcgtcc tcgacgttcg tgaactcctg ctgctgcagg
     9301 tcgcggcgca cgagcaggcc gtcgagatac tggtcggcct gcaggatcgc gagcagcgtg
     9361 ccgtacactt tgttcggcag cgcgacggtc gtcgacgcct gcacgcggtg acggacgagc
     9421 gactgaccaa agctattcga gtcgatgatc gtgatctggt agccgagcgc ggcgacctgg
     9481 cggcccaccc acgtcagctc gataccgagc cgctgatagc gatcgcgacg ctcgatgtcg
     9541 gtcgcagcgg tacacatcgg atcggccttc gtgccgggcg gacacgcgtt gatcagcgcg
     9601 acggcatcga acgcgcggtt ctcgaagccg agcgacgcga cgagctcgag gctcttcgtc
     9661 ttcgccggcg gttgccacag catcagctcg agccgcgcgt ttgccgcggg accgcgatag
     9721 tcgaagtcgt gatcgggttt gtacgtgaag tcgtgcacgc cgagcgccag cgtcagctcg
     9781 tgctcgtcgt cgttccgcat ggcgagcatt gcgtctgcgc cgaggttcac gaacgtgcgc
     9841 gcgccgatct cgtcggacaa cggaatcgcg tcgatcgcgg tgaggctgaa gccgccgagc
     9901 acgggccggt cgccgaactt gtgcagccag cgcaggttgc tcgcgaggac ggcgacgttc
     9961 tcgaccgcga cgtcgtcgtt ggcgaccacg cgcgtcagat ccgagaggtc gagcgcgaac
    10021 gcgccgccga gcaccttgcc cttgcgattc acgcgagcgc cgaatcgcat cacccaggct
    10081 gcgatttcct cggtgtcgag gccagggccg gtctcgaccc gctgaacgtt gctatcgagc
    10141 tcggcgccgg cctcgccggt gaccagccac gggttggcct ctgcgccacg tgccgctgcc
    10201 gcaagggcaa tcagcacggc aacgcgacgc atgaccgcat cgtatcccgg gccgagccgt
    10261 ggtagggcag tgggatgcgt tggctcgccg cactgttttt cgtgttcatg accggatgtc
    10321 aggcctgtaa gggcgacccg gagaagtgcg accgcgcttg ccgcaactac gcgcaacaag
    10381 tgttctggga gggggcgaac gccgagatcg acaagctgcc ggccgaccag cgggacgccg
    10441 cccgcaagca aaagatggcc gagttcgcgg ccaacctcga gaagggcgtc gatctctgcg
    10501 tcagcaagtg cgtgtccgcg aacaacacga aggacacgga ctgcttgatc ggcgcgaaga
    10561 gcgcggcggc cgccaaggcc tgcgtcaaag actaaccgcg aaggcctgcg tcacgtcgga
    10621 cggcgaccac cactagtgac ggtggtcgcg cactttcggc gcgtcgtcat ccttcttctt
    10681 gtcgtcgtca tctttgcggt gatcgcgcac gaccggcgtt gagtgcggct tcggctcgtc
    10741 acggtgatcg cgcacgaccg gctcgctgcg gtggtcacga ctgaccgtct cgtcgcggtg
    10801 gtcccgcacg gtcggcagcg gatcactccg gtggtcgcga tcgctcgtat cgctcgtgcg
    10861 gtgatcgcgc actgtcgcca cgctcgcgtc acgccacacg cccacttccc actgccactc
    10921 gataccggtc cagatccact cgccggcaac ccactcggca ccgtcgtacg gcgcatcacc
    10981 cggcacctcg ggctttggcg ccggcatggg cggtcgctcg atggcaccga gcgcgatcaa
    11041 gtactcgcgc atcgccgcgc gcaccaccag cggctgctct tccaggtact cgcgatgatc
    11101 ggcgcgcgcg tggcactctc cggtgaagta gcgaatgagc tggccgcgga tgtcgagcgc
    11161 cttcacgcgt agctgcatct ggatcgcgat ccgcgcctgc accttcggat cggcctcgcg
    11221 gcgcgcgcgt gcttcggcgt cgaggtcgcg cacgcgcacg acctcgtagc tcgcgagcac
    11281 gttcgcgtcg tccgcgatcg cgctgaccgt gcacgtgccg ctatcgagcg tgatcacgga
    11341 tgccttcgct ttcagccgac ccgcgccaac gtcgcccggc gcgagctcga ccgcatcgct
    11401 caccgtcgcg ttcgcgatcg cgacgccggc tgcattcccg accgcttggc ccgacaccgt
    11461 cgcatcgacg gtcgcgtggt tcgtctgcgc gtgcgcgtgg acgtcgggtg tgacggcacc
    11521 gatcaccgca ccggtgattc ccgcgacccg ctcgcgctcg cgctgcttct ccgctcgcag
    11581 ctccgcgatg cggcgcgcgc gatactcctc ggtcgtctcg ccggcgacgc cgctctgtgc
    11641 agcgcccggg cagatcacgg tccactcgac gagctgcgcg cgcggtacgt cgaagcgcag
    11701 cttcagctcg tacacgccgc ggggcaggtt gatgcttccc gcgggcgcgg gatcggtcga
    11761 cccgcgcgct tgcacggcgg tcgcgccgct gccaatggtt tcgcgtttca ggtgtccgcc
    11821 acacgctgtc acgagcgcga gcatcgagag gtagcccagc cgcatcgcgg tggcgtagtg
    11881 ccatcaccgt gccacgccgc tggcctcgcg tttgcgatgg ctctcgacca tgcctccagc
    11941 tctgagacat gtcgacgcac acttgcacgc ggttctgcac ggccgccagg tcctcgcggc
    12001 cgcggtgcgt gaacaacggg ctacgctccg caacaacacc gtggtccgct cactacgcct
    12061 ttggcgcctg tggatcgcga tcacgctcgc agcctgcgga ggtgggcatc gcggcgacac
    12121 gtacgcaaag gcatcgcacg agcaggagca gtgttgcgaa tcgttaagcg gcccaagccg
    12181 cgacgagtgt ctgcagaagc tcgtgcgcgt cgacgatccc gaggtcgcga agacgcgcgc
    12241 caaccaggat cagtacgcgt gcgtcgtcga gcactttgtc tgtgacccga cgagcggaca
    12301 cgcgacgcaa gccagcgcgc aggcccagct cgactgcatt caggaccttc gctaacaata
    12361 aacgtcacca acgccgcgcg tcgtgcgttc aagcatcatg cagcgcggtt ccctcctcct
    12421 cgtgatggcc gcggcgtgct ggcagaagcc gagcgctacg accggctcgg tgacgtcgtc
    12481 cgtgatggcg cggccgcagc cgaggtcggg tctggcgtgc gcgttcgtga gcgcgcgcga
    12541 ttcggaaaac cattggtcga acgcacagat ctatctggtc gacggcgttc atcgcattcc
    12601 ggtcaacctg acgaacgacg cgcaaacgaa ttgggcaccg gcgatctcac ccgatggtcg
    12661 cgagatggtg ttcgtcgcat cgcgtgggca cggtctgttc gtgctcgagc tcgaaaccga
    12721 cgagtcacgc gaggtgccga tcgcgacgcc gggtcagccc gcgtcgccgc gttggctatc
    12781 ggggcatcgc atcgcgtacg cggcattcac ggatcggaca gccgtgtggc tggtcgatat
    12841 gcggggcgat tcaccccgac agctgacgtc gcctgggccc aaggaatccg acgacggtgg
    12901 cttcgcgatg atcgatggcg gcaagcggat cgtgttctcg cgctacgatc ggctgaccgg
    12961 agagcgtcat ctctacgtgg caaacaccga tggctccgac tcgccgatcc ggatcaccaa
    13021 cacgtcgaag gtcagtgaga cgctgccgac cgtatcgcac gaaggacagc tgctcgcgtt
    13081 tcgtcgcttc gctcatacag acttctcgga tcagatccgc atcgtgtcga cgcgcgactt
    13141 ctcgctcgtg cgcgacatct cgctcgcgtc gcctgcgaag tggaacatca acggcctcga
    13201 cttcaccggc gatgatcggc agatcgtgtt cggcgccgat gcggacgacg tcggcggcaa
    13261 cttacagaac ctgcacggcg agctgttcgt cgtcggcgtc gacggcggaa accagcggcg
    13321 gctgaccaag aacgccgcgt acgatggtca gccagcctgc atgcccgccg tgataagcaa
    13381 gtaagcatgc cggtgaaccg ctgcccgcac tgtggtctcg tcaatcctgc caagacgatg
    13441 acgtgtgact gcggctactc gttcgtcgat ggcgcgccgc cgcgcttctc gcatgaccgg
    13501 cctcgcgccc gcaactcgag cgcggggtcc gtgttcgcga ctcgcgtgat gattcgcatc
    13561 gcgctggccg tgctgttcgc cggattcgcg ttcgcgattc gcgcgtgcgg ctgaccggct
    13621 accggccttt gatcgcgagc tcgatcgcgc gatcgagcac ctcgtccttg ccggcacgca
    13681 cgccagcgag cgtcggccgg atcacgatgt gcggcgtgat gccgacctgc tgcagctgct
    13741 tgccgtcgac atggcgcact tcttggcccg tgaacgacat gcgcagacca cctggcaacc
    13801 gcatcaccgt gacgtcgccg tttgcaccgt gcgtcgggct tcccacgaag gtcgcgccgg
    13861 cggcttcctg caagaacagg cacgtgtgct ccgattggct gatcgcgcgg tcgtcgatga
    13921 cgacgatgac cttgccgcgg tagatcgttg tgcccggctc gagcggcggc agcttctgca
    13981 agaagcgcgt gcgcgtgtcg gcaacttcgt cgtcggccag catgccgaag acgtgcggtg
    14041 cgaggaactg tgcgccgtac ttcgcgccgc gcgtgttgag tcgcggcgcc agcgaccacg
    14101 cgacgccgtt cggatagccg cgcatgtcga acacgatcgc tttcgtgccg tcgaggtcct
    14161 tcatcgccgc cggcacctcc ggcacggtaa gtgtgcgcag atcgatgtag ccgatgttgc
    14221 cggcgagctt cttccagtgc ggctcgctcg acggtgcatc gaacgccgct gcgtagcttt
    14281 ttgcccgcgt gaccacaaca tcacgtagct tgccgcgagc gccgcgaaca ccgagcttga
    14341 cctgtgtgcc gtcatcgcct gtgagcatgt aggccgctgc gcgttggtcg cgtgattcgg
    14401 tagtcgagcc actcgtgatc gcgcggcgtg cagcgaatgt ttccgctgcc ggcttgccgt
    14461 cgatcatctc gatcacatcg ccaaccgcaa caccgagctt cgcggtcgcc gccgcgtcga
    14521 gcaagcgcac gatcgtcggc cggttttcga caaggcgtac ctcgaacggc gcgatcccct
    14581 ttggtttcgc cgtaggatcc ggtgcgccgg gaaagacgtt gacgtggccg tcgtcgacgc
    14641 gcgcggccat ttccttcagc acgcgtaggt acgtctcgcg atctcttgcg gcgtcgaggc
    14701 gcggcagcat ctcgcgcagc acgccgtccc agtccttgat caggtagcga taaggctcga
    14761 accgatcgat cacggcccac atcctgattc cggcgagtaa gcggtgctcg cgcgatggca
    14821 gcggtgtgct cgcgtagtcg tcatcgtcgc gcacgcgcag ctgcggtaac tcgcgcggct
    14881 tcgggcgcgt gcgtttagca gccggcagct tctttgccag cgtcttcgcg agctggcgcg
    14941 cgcggaccat tggatcttgg ccggcgggga cgctgatgtc ggcggtcggt ggccccgaca
    15001 gcagctcgtt gaggcggact tgcgcacgcg aaccgccgcc gagctcgatc gtcgccgtgt
    15061 cgacgatcgc gtcatcgcgc agtgcacctt gcgcgacgat cgtcgccttg cccgtcgcct
    15121 gtagcgcgag tgcctccatc ggcagccctg cggtcgggtc ggcgacaaag acgacgtgcg
    15181 ccggtccttg cttgggcgct ggcttcggcg gccgcccgcc ggtgacgatg aacgtcgaga
    15241 agtaattgcc cgacgtggtg ccttgctgcg tgcgccaacc gcgatgttcc acgctccgca
    15301 tcagcggcca ctcgtcgatc gccggcaaca cactggcgaa ttgttcgacg atccagcgcg
    15361 acacccaggc acgcgccgtg acgttacgca gatcgatgat catgaccttc gctccagcgg
    15421 cctcggtcgc gatctgcgtc gccttctctc gcgagccctt aaaatcgagc gtgccggccg
    15481 cgaacaccga gagcttgacc tcggtgacgt ggttcgcggt cacgagccag tcggcgggcg
    15541 ccgcaatgtc gaccggcttg tcgtcgagca cgcgcgtgac cgggtcgccg atcacggcga
    15601 gcatgctctg cacggcggca tcgagctcct tctgcgtctt cgcggcctcg actttcggaa
    15661 tcgccgcgac gagcgccgca tcccagtcga tgtccttggt ctgcagcgtc gggtggaaga
    15721 acttcgcttc cgcccacacg cgtcccagcg tcatgaggcg cgtcggcgtg tcgggcagcg
    15781 gcgcgtcggc gcgggcaggc gaggcgagca ggacgactag agcagcgatc agtgcgcgca
    15841 tgccggcgca gcttaccgcg ccgaaaatga aaacgccccg acgagcgggg cgcattcgag
    15901 cgaacgagcg tggcctagaa gtccatgccg cccatgccgc ccatgccgcc gccaccgggg
    15961 gcaggcgcgg gcgcttcctt cttcggacgc tcggcgatca tcgcctcggt cgtcaggagg
    16021 agggccgaca ccgacgcggc gttctgcagc gcggtgcgca cgaccttcgt cgggtcgatg
    16081 acgccggcct tcacgaggtc ggtgtactcg agggtttgcg cgttaaagcc gaaggctccc
    16141 tgcgcttcct tcaccttgct gaccacgatc gagccgtcga cacccgcgtt catcgcgatc
    16201 tggcgcagcg gctcctcgat cgcgcggcgc acgatcgaca cgccgaacgc ctgctcctcg
    16261 ctgagcttga ccttgtcgag cgcgctctgc gcacggatca gcgcaacgcc gccaccgggc
    16321 acaacgccct cttcaacagc cgcgcgcgtc gcgtgcagtg cgtcctcgac gcgagccttc
    16381 ttctccttca tctcgacctc ggtcgcagcg ccgaccttga tgacggcgac accaccgacg
    16441 agcttcgcga ggcgctcctg gagcttctcg cgatcgtagt cgctcgtggt ctcttcgacc
    16501 gcggcgcgga tctgcttcac gcgcgcctcg atgtcgttct tcttgcctgc gccgtcgacg
    16561 atcgtcgtgt tgtccttgtc gaccgtcagg cgcttcgccg aaccgaggtc cgcgagcgtg
    16621 atgttctcga gcttgaggcc gagatcctcg gtgaccgcct ggccgccagt gaggaccgcg
    16681 atgtccttca gcatctcctt gcggcgatcg ccgaagcccg gcgccttgac ggcgcagatc
    16741 tgcagcgtgc cacgaagctt gttgacgacg agcgtggcga gcgcctcgcc atcgacgtct
    16801 tccgcgatga tgaggagcgg cttgccctgc ttcgccacct gctcgaggat cggcaggagc
    16861 tccttcatgt tcgagatctt cttctcgtgg atgaggatgt acgcgtcgtt gagcacgcac
    16921 tccatgcgct ccgtgtccgt cacgaagtac ggcgagaggt agccgcgatc gaactgcata
    16981 ccctcgacga cgtcgagctc gctgttgagc gtcttcgcct cttcgacggt gatcacgcct
    17041 tccttgccga ccttcttcat cgcctcggcg atcatctcgc cgatctcggt gtcgccgttc
    17101 gcgctgatcg tgccgacctg cgcgatgtct tccttgccct tcgtcggcgt cgtcagcttc
    17161 ttgagcgact cgacgatcgc agcaacggcg gtatcgatgc cgcgcttgag ctccatcggg
    17221 ttgtgaccgg ccgcgacgag ctttgagccc tcgcgataga tcgcctgcgc gagcacggtt
    17281 gcggtggtgg tgccgtcgcc ggcgttatcc gaggtcttcg acgcgacctc cttcaccatc
    17341 tgcgcgccca tgttctcgaa cttgttctcg agctcgatct ccttggcgac ggtgacgccg
    17401 tccttggtga ccgtgggagc gccccacgac ttctcgatca cgacgttgcg accgcggggg
    17461 ccgagcgtga ccttgaccgc gttcgccagc gtgtcgacgc cgatctgaac cttcgcgcga
    17521 gccttctcgt caaaaataat gtccttggca gccatgacgc ttctccttac gactcgatga
    17581 cgccgaggac ttcgtcctcg cgaatgatga tgtgttccac gccgtccacc ttgatctcgg
    17641 tgccggcgta cttgccgaac agaacgcggt cgcccgcctt cacgtcgagc ttcacgagcg
    17701 tgccgtcttc gaggcgcttg cccgagccga ccgcgatgac cttgccctcg agcggacgct
    17761 ccttggccga atcgggaatg atgatgccac cgcgagtctt ctcggcttcc tcgacgcggc
    17821 gaaccaggat gcgatcctgt agcggacgta cgttcatgac ctgtctccta gaaattctgg
    17881 gaattttgtt agcactcacc tcgcgcgact gctaacgggc ctggagataa ggcgtaacgc
    17941 cgcgccgcgc aaggggtgtg gtaccgggat ttctgaacag aaaccgctcc cgcgggagct
    18001 gtgcgaaccg tgcgaaagct gtgcggatct tgccctttgg gccgcgacta tgaaaatcag
    18061 atcatggacg tcacgctggc cgcatcggtg gatgagttct tccacgaggt ggtgaccgac
    18121 gcgctagcgg tggtcgatct cgacgccagc gagcctgcga gctggtacct ggttggcctc
    18181 ctcggagaga tgacccgggc tcggctcacc gacgagccgc tcggaatcaa gctggctcag
    18241 cctactgctg atgctggcga gcgcgtgcgg accctcaagc acgtcggcga tacgtcgctc
    18301 tacgtcgccg gcttcttcgc cgagtcgctg acccgctcgc ttgtcgatgt cgattactac
    18361 gtcggcctcg gtcagaacgc ctacgcacaa cttgcgcgca cgtttggcgg ccagaaatcg
    18421 attaccgagg tctacgccga gctggcggac aagttcccga agttcgtcga cgtgctcacc
    18481 gaggtccgca agcgcaccga ctttgcgact cccgacgtcg gccggctcta cgagatctgg
    18541 ctcaagaccc gcgacgagtg gatcgaaaag aagctgcgcg cactcggcgt gctcgtcgac
    18601 gtcgataaga aggtaatcca gtagtgcgga cgcttgcccg cgttcagcgt gggctcgaag
    18661 cgctgtatcg cgtcgatacc ggtgtcacgg tcgacgactt catgatcgac gagcccacgc
    18721 gtgatggcct cgtgcctacg cgccggccgc gcgagcagct gctcgtgtgc gaagaggcgg
    18781 gcgagatgtc gctcgcactg ttcatcgatc cggcggcgct ctcgaacctc gcgcagcacg
    18841 accccggcaa ccggcttggc gaccacaacc tcggcgactt cctgctcgcc gtcgaaggag
    18901 tcagccactt catctatgcg atctggtgcg cgcgggccga gcggccggtg acccagctcg
    18961 agctcgagct gcaggccgag gtggacaagt acgtcacctg tctactcacg accgaggcgg
    19021 caccatctca atccgaggtg ctccgatatc ggctgtttgt cgacgcagaa tacgagcccg
    19081 atctcgacgg tgacgagcgc gacaggtacc gcgcagccaa tgacaatgcg catcgctacg
    19141 cggcctggct cgaatccacg ttcgtggcga cgcgccgcat cccggaaatg ctaggggagc
    19201 tgagacgctt ctaccgggag gggctggccg ccaagctggc ccggatcgct cgcgcagcct
    19261 gagcctgcct gtgtaacatc ggtgcgtgga cccgagggcg cctgcagtaa aggtgttggt
    19321 ctgtgcaaag accgacgtcg gccgcgcgcg cacgaccaac gaagacgcct gctcggtcac
    19381 cgagctcgca agcggtcagg tgatcgacgc ggatagcgcc gatcgcacgg tcgatgtcgg
    19441 ccacaagggc gtgctgctcg cgctgtcgga tggcatgggt gggcaccagg cgggtgaggt
    19501 cgcgagcgcg ctcgtgctcg agtcgctcgg cagcgagctg acgcaagacg cgaagggccc
    19561 ggtgcaccgc cagctcgagg acgcggtaca tcgcgcgaac cgtgcggtgc tcgaagcggc
    19621 gaagagcgcc gaccgtcacg gcatggg
//
LOCUS       DASUFX010000451        18503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1045457, whole genome shotgun sequence.
ACCESSION   DASUFX010000451 DASUFX010000000
VERSION     DASUFX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18503
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045457"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFV99_18140"
     CDS             <1..1180
                     /locus_tag="VFV99_18140"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX5061296.1"
                     /translation="AARGIAAPNAVTQLAELDTQEREARARRIDAEHEVDLAAAALAR
                     LDERIARAEAEAGEQAARLVIDLIADAPGEATVTASYIVPGAAWRPYHRAQLARESGK
                     LDWQTTACVWQATGEDWTDVELTCSLERPSLGVEPPSLVDDELRARRKPDSVVVEARE
                     QQQQDTGLGGAGPLQVPGIDDGGVGLRLTAGKVTVKHDGVPHRVPVGGFSATAQVSLV
                     GIPLRSPWVHVRARIINTSTTPLLAGPVDLIMASGYVGRAEIGFVAANEKFYLGFGPE
                     ADVRMHRDEERIRDEAGILGGWNTQTVRVAVRLSNLGAQKREVTITERIPISEVEQVE
                     VNASAPDAYELDEETTQVTARALDDKGLVSWAVELPPLGRRAVTLEYKVKSQRGVSIT
                     "
     gene            1192..1533
                     /locus_tag="VFV99_18145"
     CDS             1192..1533
                     /locus_tag="VFV99_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061297.1"
                     /translation="MPLSRRTALIIPLVLCVVAILQDVAMYKMRQHIHNVYQRAALVL
                     LLNGVLFGIAAAFVAPRLQKFLQRVGKESRVAGDFGLWIFYAGAYGLLFYVYVVIERH
                     GVAALLPASVR"
     gene            1536..2450
                     /locus_tag="VFV99_18150"
     CDS             1536..2450
                     /locus_tag="VFV99_18150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061298.1"
                     /translation="MSAIVEMFANRVRKNARHLGKWAKRDKITCYRIYDRDIPEVPVT
                     LDTYEGVLVLNDYRIDQTDTAWLDALGAAAKDVLGATEVFAKQRERLAHRGDGGQYER
                     LDERGAWRVVSEGGHQFRVNLSDYIDTGLFLDHRITRARAAAEPATSMLNLFCYTGAF
                     SVYGAAAGMQTTSVDLSKTYLDWARQNLELNKLDGELIHADVREFLTDARRTGRRWDL
                     AVVDPPTFSNSKRMDYTWDVQRDHAALLDDVAAVTTKVIWFSTNLRRFKLAWTNPRAQ
                     VRDETHATVPPDFRDKRIHHAFRITVSP"
     gene            complement(2456..3109)
                     /locus_tag="VFV99_18155"
     CDS             complement(2456..3109)
                     /locus_tag="VFV99_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061299.1"
                     /translation="MQSFVNFFGHAAVASWLAPSLDAVASGHALGSMLPDFATMSGAR
                     LAENHLDVSIAQGIDLHHTTDSAFHHLPPVLALMRELDERLDRAGCARGPRRAVAHIG
                     VELLLDGVMVDDEAYRDSYLAAIAHDTAGIRWRYDGDTARFAMLITRLRAHGVPEDLR
                     RPDAIAVRLHRMLAHRPRLAPSPADLVAIKSSLVAHKPRVDVATDTVLRALRAALRA"
     gene            3234..4166
                     /locus_tag="VFV99_18160"
     CDS             3234..4166
                     /locus_tag="VFV99_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061300.1"
                     /translation="MLRAPGVSDPAHYHRRLTWLAIVHPALFASAVAGIALLVWRGEL
                     FVTLAQRSNVETLTIAFFLVFFGYFLVVAAPGALGSARIGWYRLRERVARDRERIGKQ
                     REAALGERGDGSSAAFDKVIELASAPGHAWEVEIRDNVGSLGRLQFDGVRVRHLDAFR
                     GGSNTLFGYVEDKLAELAGVEVTLVQWSSTDEEALLAYVANADALRAIGEKLGTRTWP
                     VVTLTDDARDTLQRELAALAPALREEAFLPDWEFEGEHKLPIIPEPLGIISLTRSERR
                     VDPLSSLTAALVVVVIVLGLLCYFIARPPWIPGH"
     gene            4148..4453
                     /locus_tag="VFV99_18165"
     CDS             4148..4453
                     /locus_tag="VFV99_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061301.1"
                     /translation="MDTRALIACLLVACTGPKPHAEVVTVAPSPVPGHERVTLAVRND
                     GRHGEVTLEITLRGASGSVLHDTHHIDVQADERISVVVDIAAPADAYTATVEAQYPD"
     gene            4517..6001
                     /locus_tag="VFV99_18170"
     CDS             4517..6001
                     /locus_tag="VFV99_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5061302.1"
                     /translation="MVVVGIDLGTQSLKAVVCNERFDILGSFGVAQTTAYPRPGWAEQ
                     DPRAWEAALAPAIEGALMAAGVHAQDVAAIGLSGQLDGCVAVDTHGAPLHPALIWQDK
                     RASDHLRADATRLFQITGQIADASHMAAKARWLRAAGIAAARFHQPVTYLVERLTGAA
                     VIDPAHASTTMLYDLLAGTWSAELLAAFELDASMLPTLQPAHAVAGVLTAQGAQLTGL
                     PPGIRVAVGTGDDFATPIGAGIVSAGSIAVTIGTAEVVGAIADTPVFDVPAVRAASDP
                     WRELAEPMVETHAYPTGTFFVENPGWLAGGAVRWAIETFGFADEAAFDRAAAQAPAGA
                     AGVTFVPSLTGAMTPVWRGHARGTLHGLSNAHDRAHIARAVLEGIAFASRDVARRLVA
                     LGLPAREVIVLGGGSRSGLWMQLRADALGLVHRVANRTDTCAIGAAMLASVAIGTHAD
                     IYTAAANAAQHPTTFEPRESLEEAYGRYRRLVAALAPLADQPWA"
     gene            6042..6458
                     /locus_tag="VFV99_18175"
     CDS             6042..6458
                     /locus_tag="VFV99_18175"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5061303.1"
                     /translation="MRRLLFVGLLAGCLDGGGCSTEPPPECLAVAPDTACLPQYPPTF
                     TNVYKNTLQPNCGYMDGSCHSASGRAGGLSFQDQDTAYAGLLNGIRVKPMNPTCSEMI
                     VRATSPGKDYQMPPGDALSEPEQCALVQWVQMGAMP"
     gene            6431..7930
                     /locus_tag="VFV99_18180"
     CDS             6431..7930
                     /locus_tag="VFV99_18180"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5061304.1"
                     /translation="MGTDGSDAVKYVLFLLALAACDGCDGGKYSVERLQNPETCKDCH
                     PKHYDQWSGSMHAYASDDPVFVAMNKRGQRDTNGELGNFCLTCHAPMALELGLADGAT
                     FDPAALPPEARGITCFFCHNVEQVTDDHNNPIKLAMDQTMRGGAKNAVDTPAHHSKYD
                     AELMASKTNQSTMCGSCHDLVTPAGVHLERSFKEWKESIWATNDPDPLAFLPQTCSNC
                     HMFPTDGVIADNPTLHVKSRTLGFHEHMWPAIDQALTPFPQMTEQAAGIKRDLADSIG
                     IKGPKPRNSNETPGGICLDPPGLLNVRVDSFSVGHMFPSGAAQDRRMWVHVVVKDAGG
                     TILYERGKLDPGVDPEQSTDLTLNCAPPTNPLIPSQCGSFYDRTYKSDGTQAHFFWEV
                     DRYDSFLIRPAVTRDPNAVGYDHSTTVQYQVAPAVFSGADRIEAELLMRPLPFEMLHQ
                     LEQSGDIDPSIRTTLEAMPPIAVGRKSIWTKATAGMVGSPAENTNCNPE"
     gene            complement(7940..8404)
                     /locus_tag="VFV99_18185"
     CDS             complement(7940..8404)
                     /locus_tag="VFV99_18185"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX5061305.1"
                     /translation="MRSSTKSVVMLGLAACGTNEGDDMTEVDCSMVKNVDTFVVGLEH
                     PGEKGTYDFKLMSAEPAPPARGFNTWVVQVNSMNAGVVGAPVDGATLKVTPFMPAHMH
                     GSGVEVQITPMTEPGQYQLDPVNLWMQGVWETTVRATLDPSSDSTVFKFCIP"
     gene            complement(8496..9134)
                     /locus_tag="VFV99_18190"
     CDS             complement(8496..9134)
                     /locus_tag="VFV99_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061306.1"
                     /translation="MQRALAHAYIHRDAPLPTLRLSLGYMTSTACTTDEVTGLGHRMT
                     LLGVAASEGVAYVVEDINEVEAPIIYRLWLRGPRVGHLVPIHAWYEQSHDAAEIRVRI
                     AAIATTLEPAGNTTKEAWMLSTRIVQRRALRIAGKDEPPIRKFALQLRVEPVSGVGPS
                     GHTTVTAFLRPGATLAEVWSIPGENMAIARVTYTGIPSGIGLAKDTMVLLTE"
     gene            complement(9303..10643)
                     /locus_tag="VFV99_18195"
     CDS             complement(9303..10643)
                     /locus_tag="VFV99_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061307.1"
                     /translation="MRYGNFITLFPDQEIEIAGSYKDGKLDGAWQRHYPGGAIAETGT
                     YVAGLRDGVWKQLGPSGNVLGEYTMKQGTGKQKRWFDDGPLYSEITLSKGVPNGLARI
                     FDHNGAVVVVANMFAGKLHGKHVAGAKNTLRIEESFVRGVRRGPRKIWQFWTLLIDEA
                     YDTKGKLDGQFTMWRDRKVQRVTGVYEHGKRTGTWVWNDKNNKKEREGDYSDGQKRGL
                     WSEWGDDKLSFQGMFTDGKPDGDFVYYDTKTGNELGRFTITNGTGTMLTFYPNQKIAS
                     KTPMVNGQIDGKYEELTIRGKTVVEGRYSKDQKHGLWREQTELGVPTLEQHWKRGKLD
                     GAWQKFIDGKVAASATYKDGLADGPYTEYRGDKKSVVGQFVADKRSGTWTSYDADGVV
                     TLTATYKDGVLEGPWRQLALGSVLEGEMRAGRRVGTWTQTDRAGQKTSVTYQTP"
     gene            10967..11545
                     /locus_tag="VFV99_18200"
     CDS             10967..11545
                     /locus_tag="VFV99_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3996 domain-containing protein"
                     /protein_id="HEX5061308.1"
                     /translation="MKVTRLVALSVLMAVAAAPAVEARPRPAGGGNLKKFSANKTFGA
                     GLELGSPSGLTGKYFLSDSNALDFGLGYIYHSYYYGDDGIHLYLDYLWHPASLVSAEA
                     FELPFFIGVGGRYWDFDYCDGRGCINYGGSAIGVRIPLGIAFDFNTVPLDIALNIVPT
                     IDFIRGDYYDRVDHHRAHFGFDVSIAIRYWFK"
     gene            11599..11997
                     /gene="panD"
                     /locus_tag="VFV99_18205"
     CDS             11599..11997
                     /gene="panD"
                     /locus_tag="VFV99_18205"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5061309.1"
                     /translation="MSHRLHLFKAKIHRATVTHADLDYEGSVTISGELMDAAGMLEHE
                     QVHIWNVTRGTRLVTYTLRGDNDSGIVCINGAAAHLMKPGDKVILATFAEVDAADAAS
                     WKPMVVFVDDANKIVGEGAEVAGPKRRLTA"
     gene            complement(12019..12669)
                     /locus_tag="VFV99_18210"
     CDS             complement(12019..12669)
                     /locus_tag="VFV99_18210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5061310.1"
                     /translation="MLLVLLAACGATHTPKRPTTGAITGLVRDVASGEGLEHASLRLS
                     NGLTTESNKAGMYTLDDLTPGRYTLLAEYAGQPITIKNIDVTRGEATYVDVKITLGAT
                     SPVTIDFADPQNAITHYKSKQEIAVIEGAVSEMNTHERVAGAVVTVVGGPRGEVLQTV
                     TDDQGRYRFDGVEPGTYVVSAYYSVGGHGQIEVRRSGIEVEHAQGVIVPLVVETTR"
     gene            12784..13779
                     /locus_tag="VFV99_18215"
     CDS             12784..13779
                     /locus_tag="VFV99_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061311.1"
                     /translation="MLRAALLGLLAVSAVSCKSGGDAPKSQPGVAAGKVVEVTGTVTV
                     RHNDVAQPLAKGATVEGDDIIETGADGNAIIELSHNLARWELGPNKTSKVRESTAWGL
                     AKKSGAVAVVQQDTAAAGRPAERSAADTSVSARADEAAPAPGAAPAPAAAAPPAPEAA
                     PAAAVAPPPPPPAPEKAAAPTRQRSVRGDDGLLDKESGGGGAPPAPPKITTRGGVAKD
                     GVGAGGGGAAKDSGSVGATANITADAAKPMTPAEADKAATDVVLKNEKALKACLTKDA
                     QSVNVTIKVAANGTATAVVSGKAEIPAAVQRCVKAAVGKMAFAKAATTVSMEISR"
     gene            complement(13803..14666)
                     /locus_tag="VFV99_18220"
     CDS             complement(13803..14666)
                     /locus_tag="VFV99_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5061312.1"
                     /translation="MRAHLFDGTYELFRAWFGAPPAQHEGREVGATRAFVQSLARVLR
                     SGAVTHGGVAFDTVIESFRNDLFLGYKTGEGLEPNLVAQFPLVERAAEALGLTAWRMI
                     EFEADDAIATAAAHLAKDSRVEQVRIVAVDKDLCQSVIGTRVVAWDRYKDAILDEPGV
                     IAKHGVPPESIPDLLGLVGDTADGIPGLEGWGKSSAAKVLTVWKHIQHIPDDAAKWNV
                     AGLRGADRLATSLRDSRELAMLYQKLATLRTDCPIVCDLDALAWRGPNREALAALCAE
                     IGLAPDSVRTS"
     gene            14692..16413
                     /locus_tag="VFV99_18225"
     CDS             14692..16413
                     /locus_tag="VFV99_18225"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5061313.1"
                     /translation="MSAASSPLAADAVAGIRDARLRDIVADHWEYMMKWAPTWATTLG
                     DHRYDDRLAPRDAAAIAEMDAEHAALLARISDIDIATLGDTDAVTCKLLRGRLEAEQA
                     LAQLKLHEHHLVDAGMSTVFDELSYLIEMHVVRSHADAQNVVARMRMGAKLFDDKVAH
                     LAVGLATRRVSSAEKIRRVIGQLDAELEKPVETWAMASPAWADREPELHAELRSIIAT
                     EIHPAVVRMRDFLRDRLLPNARGDNEGLCALSDGDAAYRASILYHVGLPIDPRTLHEL
                     GLREIARTDRELTELGRRVFGTSDLASTFARLRDDRALYFGSGAELLTAAQFALDRAK
                     AAVPSYFSVLPITDCVMREIPDYEAPYSTIAYYRQPHYDGTKPGEYFVNTYKPETRPR
                     YELEALTWHESIPGHHLQIALAQELGELPAFRKLDGSTAFVEGWALYTEHLAEEMGLY
                     TADLDRIGRASYDAWRASRLVVDTGIHALGWTRAQAEAFMREHTALTPINISNEVDRY
                     IGWPGQALSYKFGQLEIIKLRGEAEQKLGARFDLKAFHAIVLGAGAVTLPVLADRVNA
                     WITANQS"
     gene            16389..17726
                     /locus_tag="VFV99_18230"
     CDS             16389..17726
                     /locus_tag="VFV99_18230"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5061314.1"
                     /translation="MDHRQPELTRGLGPWAAASLVVGTIIGTGIFLKTSVMAATAGSA
                     VWVLAAWACAGGLSLFGALTYAELGAMFPQAGGEYVFLRHGYGPGMGYLYAWNRFWIA
                     TPGSIAAYAVGTTTFLGGVVDIDPDSKLIAIGMIVLFTLANCLRVRTGGALQTVLTAM
                     KVVMIGGLAFGALFFARGGDWSRVGDSGPGFPGWSPFGAMVLAALWAYDGWNNLPMAA
                     GEVRNPQRTLPRAIVWGSLGVVAIYALVNIGYFHALPFTDVAAKSSTSIAERTATTFL
                     GVPAQALLMAAMAISAISAMNGSMLTGARVPYAVARDGLAPKSLAHVAEGARVPVVAV
                     LVQGGLGVLYALSGKFDELTDAVVFASWLFYGLNAGTVLLLRIREPDRERSYRVPGFP
                     VVPVIFMVLALLLLVNAIVTSPRASALGLVMTALGGIVYTGYYRPRSKASSSS"
     gene            complement(17690..18121)
                     /locus_tag="VFV99_18235"
     CDS             complement(17690..18121)
                     /locus_tag="VFV99_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061315.1"
                     /translation="MVSTFAQLEASRDLDGEVVGAGSESATVVVVMASWCSHCRDEIA
                     MFDRVRVAHPHVRWLAVNYKLHEEYDQRGNAEAIRALARTVPWLRVIPAGDELFTAVG
                     RPQKIPTVLVFDHGGAELANFQREHRAPPAQDELEALLRGR"
     gene            18263..>18503
                     /locus_tag="VFV99_18240"
     CDS             18263..>18503
                     /locus_tag="VFV99_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061316.1"
                     /translation="MAPVITFVASKYQEDGLETLYLGAVAFGVTLLAASFLLGGKDTD
                     HGDGGHADMGLAWAPFASLRFWIFLFTFGGGAGYAL"
BASE COUNT         3026 a         6150 c         6179 g         3148 t
ORIGIN      
        1 agcagcacgt gggatcgcgg cgccgaacgc ggtcacccag cttgccgagc tcgacacgca
       61 ggagcgcgaa gcacgcgccc gccgtatcga tgccgagcac gaggtcgacc tcgcagcggc
      121 cgcactcgcg cggctcgacg agcgcatcgc gcgcgccgaa gcggaagcgg gcgagcaggc
      181 cgcgcgtctc gtgatcgatc tgatcgcgga tgcgccgggc gaagcaaccg tgaccgcgag
      241 ctacatcgtg ccgggggcgg cgtggcgccc gtatcaccgc gcacagctcg cgcgcgagag
      301 cggcaagctc gactggcaga ccactgcgtg cgtgtggcaa gcaaccgggg aagactggac
      361 ggacgtcgag ctgacctgct cgctcgagcg gccatcgctc ggcgtcgagc cgccatccct
      421 cgtcgatgac gagctgcgcg cgcgtcgcaa gccggattcg gtcgtcgtcg aagcgcgcga
      481 gcagcaacag caggacaccg gcctcggcgg cgccggaccg ctgcaagtgc ccggcatcga
      541 cgacggtggc gtcgggctac gcctcaccgc tggcaaggtg accgtgaagc acgacggcgt
      601 gccgcatcgc gtgccggtcg gcggcttctc ggccacggct caggtctcgc ttgtcggcat
      661 tccgcttcgc tcgccgtggg tgcacgtacg cgcgcgcatc atcaacacca gcacgacgcc
      721 gctgctcgcc ggacccgtcg atctgatcat ggcgtcgggc tacgtcggcc gcgccgagat
      781 cggcttcgtc gcagcgaacg agaagttcta tctcggcttc ggtcccgagg ccgacgtgcg
      841 catgcaccgc gacgaggaac gcattcgcga cgaggccggc attctcggcg gctggaacac
      901 gcagaccgtt cgcgtcgcgg tgcgactctc gaacctcggc gcgcagaagc gcgaggtcac
      961 gatcaccgag cgcatcccga tctcggaggt cgagcaggtc gaggtcaacg cgtcggcacc
     1021 cgatgcgtac gagctcgatg aggaaaccac gcaggtcacc gcgcgtgcgc tcgacgacaa
     1081 gggcctcgtg tcgtgggccg tcgagctgcc gccgctcggt cgtcgcgcgg tcacgctcga
     1141 gtacaaggtc aagagtcagc gcggcgtctc gatcacgtag actgtgtcgc catgccgctg
     1201 agccgccgca ccgcgctgat cattccgctc gtgctctgcg tcgtcgcgat cctgcaagac
     1261 gtcgcgatgt acaagatgcg ccagcacatc cacaacgtct atcaacgcgc agcgctcgtg
     1321 ctgttgctca atggcgtgct gttcggcatc gccgccgcgt tcgtcgcgcc acgcctgcag
     1381 aagtttctcc agcgggtcgg caaggagagc cgcgtcgcgg gcgatttcgg cctgtggatc
     1441 ttctacgccg gcgcgtatgg cctcttgttc tacgtctacg tcgtgatcga gagacacggc
     1501 gtcgccgcgc tcttgcccgc gagcgttcgc tgaccatgtc cgccatcgtc gagatgttcg
     1561 cgaaccgcgt gcgcaagaac gcgcgccatc tcggcaagtg ggccaagcgc gacaagatca
     1621 cgtgctaccg catctacgac cgcgacatcc ccgaggtgcc cgtaacgctc gacacgtacg
     1681 agggcgtgct cgtcctcaac gactaccgca tcgatcaaac cgacaccgcg tggctcgacg
     1741 cgctcggcgc cgccgcgaag gacgtgctcg gtgcgaccga agtgttcgcg aagcagcgcg
     1801 aacggctcgc gcaccgtggc gatggtggcc agtacgagcg cctcgacgaa cgcggtgcct
     1861 ggcgtgtcgt cagcgaaggc ggtcaccagt ttcgcgtcaa tctcagcgac tacatcgaca
     1921 ccggcctgtt tctcgatcac cgcatcacgc gcgcgcgtgc ggcggcggag ccggcgacat
     1981 cgatgctgaa tctcttctgt tacaccggcg cgttctcggt ctacggcgcg gcggcgggca
     2041 tgcagacgac gagcgtcgac ctatcgaaga cgtatctcga ctgggcgcgc cagaacctcg
     2101 agctcaacaa gctcgacggc gagctgatcc acgccgacgt gcgcgagttc ctcaccgatg
     2161 cgcgccgcac gggccgccgc tgggacctcg cagtcgtcga tccgccgaca ttctcgaact
     2221 ccaagcggat ggactacacc tgggatgttc aacgtgatca cgcggcgttg ctcgacgatg
     2281 tcgcggcggt gacgacgaag gtgatctggt tctcgacgaa tctgcgcagg ttcaagctcg
     2341 cgtggaccaa cccacgcgcg caggttcgcg acgagacaca cgcgacggtg ccaccggact
     2401 tccgcgacaa gcgcatccat cacgcatttc gcatcacggt atccccctag aggggctacg
     2461 cgcggagcgc ggcgcgcaga gcgcggagca ccgtgtcggt ggcaacgtcg acgcgcggct
     2521 tgtgcgcgac aagcgagctc ttgatcgcga ccaggtcggc aggcgagggc gcgaggcgcg
     2581 gacgatgcgc gagcatgcga tgcagccgta ccgcgatcgc atcgggccgg cgcagatcct
     2641 cgggcacgcc gtgcgcgcgc agccgcgtga tgagcatcgc gaaccgcgcg gtgtccccgt
     2701 cgtagcgcca gcggatcccg gcggtgtcat gtgcgatcgc ggcgaggtag ctgtcgcgat
     2761 atgcctcgtc gtcgaccatc acgccatcga gcagcagctc gacgccgata tgagcgactg
     2821 ctcgccgcgg accgcgtgcg cagccggcgc gatcgagtcg ctcgtcgagc tcgcgcatga
     2881 gggcgagcac cggtggcagg tgatgaaacg cgctgtcggt cgtgtggtgg agatcgatgc
     2941 cttgcgcgat cgacacgtcg aggtggttct cggccaggcg cgcgccgctc atcgtcgcga
     3001 agtcgggcag catcgagccg agcgcatgac cgctcgccac ggcgtcgagg ctcggcgcca
     3061 accagcttgc gactgcggcg tggccgaaga agttcacgaa gctctgcaag gttacaacaa
     3121 cgcgcgcgcg gatccgagcc gttcgccgac ggcacgccga gtgcacttac gtgtgctgcg
     3181 cgatgtcgcc agcgcgccgt catcttcgag gaaaccgagc gatcgcgcac gccatgctgc
     3241 gtgcgccggg cgtctcggac ccagcgcatt accaccgccg tcttacgtgg ctcgcgatcg
     3301 tgcacccggc actgtttgcc agcgcggtcg ctggaatcgc gctgctcgtc tggcgcggcg
     3361 agctgttcgt gacgctcgca caacggtcga atgtcgagac tctaacgatc gcgttcttcc
     3421 tcgtgttctt cggctacttc ctcgtcgtcg ccgcacccgg cgccctcggt agcgcgcgga
     3481 tcggttggta ccggctgcgc gaacgcgtgg cgcgcgatcg cgaacgcatc ggcaagcagc
     3541 gcgaggctgc actgggcgaa cgcggtgacg gtagctccgc ggcgttcgac aaggtgatcg
     3601 agctggcttc cgcgccaggc cacgcatggg aggtcgagat ccgcgacaac gtcggctcgc
     3661 tcggccggtt gcaattcgac ggagtgcggg ttcgtcatct cgacgcgttc cgcggcggct
     3721 cgaacacgtt gttcggctac gtcgaggaca agctcgccga gcttgccggc gtcgaagtaa
     3781 cgctcgtgca atggtcatcg accgacgagg aggcgctgct cgcctacgtc gcgaatgccg
     3841 acgcgttgcg tgcgatcggc gagaagctcg gcacgcgcac gtggccggtc gtgacgctca
     3901 ccgacgatgc acgcgacacg ttgcaacgcg agcttgccgc gcttgcgccg gcgcttcgcg
     3961 aggaagcatt tcttccggac tgggagttcg agggcgagca caagctgccg atcattcccg
     4021 agccgctcgg catcatcagc ctgactcggt ccgaacgccg cgtcgatccg ctgtcgtcgc
     4081 tgaccgctgc actcgtcgtc gtcgtcatcg ttctcggctt gctctgttac ttcatcgcga
     4141 ggccaccatg gatacccggg cactgatcgc atgcctgctg gttgcttgca ccgggccgaa
     4201 gccacatgcc gaggtcgtga cggttgcgcc gtcgccggtg ccaggccacg agcgcgtcac
     4261 gctcgcggtt cgcaacgacg gcagacacgg cgaggtcacg ctcgagatca cgctccgcgg
     4321 cgccagcggc agcgtgctgc acgacaccca ccacatcgat gtgcaggccg acgagcgaat
     4381 atcggtcgtc gtcgatatcg cagcacccgc cgacgcgtac acggccacgg tcgaggcgca
     4441 gtatccggac tgacgcaagg cacacgcgtc cgtgcgcggt gagcgccgga gtcgcaaact
     4501 ggcgtagcat cgcggcgtgg tcgtcgttgg tatcgatctc gggacgcaga gcctcaaggc
     4561 ggtcgtgtgc aacgagcgct tcgacatcct cggctcgttc ggtgtcgcgc aaacgacggc
     4621 ctacccgcgc ccgggctggg ccgagcagga cccacgcgcc tgggaggccg cgctcgctcc
     4681 cgccatcgaa ggcgcactga tggcagccgg cgtgcacgcg caggacgtcg ccgcgatcgg
     4741 cctctcggga cagctcgacg gctgcgtcgc cgtcgacacg cacggagcgc cgctgcaccc
     4801 ggcgttgatc tggcaagaca agcgcgcgag cgatcatctg cgcgccgatg ccacgcggct
     4861 gttccagatt accggccaga tcgccgacgc gagccacatg gcagcgaagg cgcgctggtt
     4921 gcgcgcggcc ggcattgccg cggcacggtt tcaccagccg gtcacgtatc tcgtcgagcg
     4981 gctcaccggc gctgccgtca tcgatccggc gcacgcgtcg acgacgatgc tctacgacct
     5041 gctcgccggc acgtggtcgg cggagctgct cgcggcgttc gagctcgacg cgagcatgct
     5101 gccgacactg caacccgcgc acgcggtcgc tggcgtgctc accgcgcagg gcgcgcagct
     5161 cactggcttg ccgcctggga ttcgcgtcgc agtcgggacc ggagacgatt tcgcgacgcc
     5221 gatcggcgcc ggcatcgtca gcgcgggatc gatcgcggtc acgatcggca ccgcggaagt
     5281 cgtcggcgcg atcgcggata caccggtctt cgacgtgccg gccgtgcgtg ccgcgagcga
     5341 tccatggcgc gagcttgccg agccgatggt cgagacgcat gcgtatccga ccggcacatt
     5401 tttcgtcgag aacccgggat ggctcgcggg cggcgccgtg cgctgggcga tcgagacgtt
     5461 cgggttcgcg gacgaagccg ctttcgatcg cgcggccgca caagcgccgg caggtgcggc
     5521 cggcgtgacg ttcgtgccgt cgctgaccgg cgcgatgacc ccggtgtggc gtggtcacgc
     5581 gcgcggcacg ttgcacgggc tatcgaacgc gcacgacagg gcgcacattg cgcgcgcggt
     5641 gctggaaggc atcgcgttcg cgagccgtga tgtcgcgcgg cgacttgtcg cactcggttt
     5701 accggcgcgc gaagtgatcg tgctcggcgg tggcagtcgc agtggactgt ggatgcagct
     5761 acgcgccgac gcgctcggcc tcgtgcatcg cgtggcaaat cgcacggaca cctgcgccat
     5821 cggcgccgcg atgctggcgt cggttgccat cggcacgcac gctgacatct acacagccgc
     5881 tgcgaacgct gcgcagcacc cgacgacgtt cgagccgcgc gaaagcctcg aagaggcata
     5941 cggccgctat cgccggctgg tcgccgcgct cgcgccgctc gcagatcagc cgtgggcgta
     6001 gtcactacga cggcgtgaca tcctgggcta cattgcggcc gatgcgacgg ttgttgttcg
     6061 tcggcttgct tgccgggtgc ctcgacggcg gtggttgctc gaccgaacca ccgcccgagt
     6121 gcctcgctgt tgcgccggat actgcctgct tgccgcagta cccgccgacg ttcacgaatg
     6181 tctacaagaa cacgctgcag ccgaactgcg gctacatgga tggctcgtgt cactcggcga
     6241 gcggccgcgc gggcggactg tcgtttcaag accaggacac cgcgtacgcg ggcctgctca
     6301 acggcatccg cgtgaagccg atgaatccga cgtgcagcga gatgatcgtg cgcgcgacgt
     6361 cgcccgggaa ggactaccag atgccgccgg gtgatgccct gagcgaaccc gagcagtgcg
     6421 cgctggtcca atgggtacag atgggagcga tgccgtgaag tacgtgctgt tcttgctcgc
     6481 gctcgcggcc tgcgatggct gcgacggcgg aaagtattcg gtcgagaggc tgcagaaccc
     6541 ggagacgtgc aaggactgcc atccgaagca ctacgatcaa tggtcgggct cgatgcacgc
     6601 gtacgcatcg gacgatccgg tgttcgtcgc gatgaacaag cgcggccagc gcgacacgaa
     6661 cggcgagctc ggcaacttct gtctcacgtg tcatgcgccg atggcgctcg agctcgggct
     6721 cgccgacggc gcgacgttcg atcctgcggc gctcccgccc gaggcgaggg gtataacgtg
     6781 cttcttctgc cacaacgtcg agcaggtcac cgacgatcac aacaatccga tcaagctcgc
     6841 gatggatcag acgatgcgcg gcggtgcgaa gaacgcggtc gacacgccgg cgcatcactc
     6901 gaagtacgac gccgagctga tggcatcgaa gacgaaccag tcgacgatgt gcggcagctg
     6961 ccacgatctc gtcacgccgg cgggcgtgca cctcgagcgc tcgttcaagg agtggaagga
     7021 atcgatctgg gcgacgaacg atcctgatcc gctcgcgttc ctgccgcaga cttgtagcaa
     7081 ctgccacatg ttcccgaccg acggcgtcat cgccgacaac ccgacgctgc acgtgaagtc
     7141 gcgcacgctc ggtttccacg agcacatgtg gccagcgatc gatcaggcgc tcacgccgtt
     7201 cccgcagatg acggagcagg ccgcaggcat caagcgcgat ctcgccgatt cgatcggcat
     7261 caaaggcccg aagccgcgca actcgaacga gacgcccggt ggcatctgtc tcgatccacc
     7321 ggggctactc aacgttcgcg tcgattcgtt cagcgtcggg cacatgttcc cgagcggcgc
     7381 tgcgcaagat cgccgcatgt gggtacacgt tgtcgtgaag gatgccggcg gcacgatcct
     7441 ttatgaacgc ggcaagctcg acccaggcgt cgatcccgag cagagcaccg acctgacgct
     7501 caactgcgcc ccgccgacga atccgctgat cccgagccag tgcggctcgt tctacgaccg
     7561 cacctacaag agcgatggca cgcaggcgca cttcttctgg gaagtggacc gctacgacag
     7621 cttcctgatc cgcccggcgg tcacgcgcga tccgaacgcc gtcggctacg accactcgac
     7681 gacggtgcag tatcaggtcg cgccagcagt gttcagcggt gccgatcgga tcgaggccga
     7741 gctgctcatg cgaccgttgc cgttcgagat gctccaccag ctcgagcagt cgggcgatat
     7801 cgatccgagt atccggacga cgctcgaagc gatgccgccg atcgcggtcg gtcgcaagag
     7861 catctggacg aaggccacag caggcatggt cggttcgcca gccgagaaca cgaactgcaa
     7921 tcccgagtga gcgtcgcgct cagggaatgc agaacttgaa tacggtgctg tcgctgctcg
     7981 gatcgagagt cgcgcgcacc gtcgtctccc acacgccctg catccacagg ttcaccggat
     8041 cgagctggta ctgaccgggc tcggtcatcg gcgtgatctg cacttcgacg ccactgccgt
     8101 gcatgtgcgc cggcatgaac ggcgtcacct tgagcgttgc cccatcgact ggagcgccga
     8161 cgacgccggc attcatcgaa ttgacctgta cgacccacgt gttgaagcca cgcgcgggcg
     8221 gcgccggctc cgcagacatg agcttgaagt cgtaggtgcc cttttcgccc ggatgctcga
     8281 gcccgaccac gaacgtatcg acgttcttga ccatcgagca gtcgacctct gtcatgtcgt
     8341 cgccctcgtt cgtgccgcac gcggcgaggc cgagcatcac cacagacttc gtagacgaac
     8401 gcacgatcga gatacgcatg acagagcgga ggttacggca ggtacgtgtc gccggggagt
     8461 gcggatcgct cacccgggct aacacgctga tacccctact ctgtgagcaa caccatcgtg
     8521 tcttttgcca ggccgattcc gctcgggatt cccgtatagg tcacccgcgc gatcgccatg
     8581 ttctcgcccg gaatcgacca cacttccgcg agcgtcgcac cgggccggag aaatgccgtc
     8641 acggtcgtgt ggccgctcgg cccaacgcca ctgaccggct cgacccgcag ctggagcgcg
     8701 aacttgcgaa tcggcggctc gtccttgccg gcgatgcgaa gcgcacggcg ctgcacgatg
     8761 cgcgtcgaca gcatccacgc ttccttcgtc gtgttgcccg cgggctcgag cgtcgtcgcg
     8821 atcgccgcga tgcgcacacg aatctccgcc gcgtcgtgcg actgctcgta ccacgcgtga
     8881 atcggcacga ggtgaccgac gcgcggaccg cgcagccaca gccgataaat gatcggcgcc
     8941 tcgacctcgt tgatgtcttc gacgacgtac gcgacgcctt cggaagccgc gacgccgagc
     9001 agcgtcatcc ggtggcccag gccagtgact tcatcggtcg tgcatgcggt cgacgtcata
     9061 tagccaagcg agagccgcag cgtcggcagc ggcgcatcgc gatggatgta cgcgtgagct
     9121 agcgcgcgct gcatccgacg agtgcgcgcg agggcgatga gagcagtctg ggtcttggcg
     9181 tcacggttca cggcgggtct cagagctatg aacgcgcgag aacgccaggg gttgcactcg
     9241 acgtgacgaa tgcaacgtcc gggaataacg tcgtgtctag accggttgaa tgccgaaccg
     9301 ggtcagggcg tctggtacgt caccgacgtt ttctgacctg cccgatcagt ctgggtccag
     9361 gtcccgacgc ggcggccggc gcgcatctcg ccttcgagca cgctgccaag agcgagttgt
     9421 cgccacgggc cttcgagcac gccgtccttg tacgtcgccg tcagcgtgac gacaccatcg
     9481 gcgtcgtagc tcgtccacgt accgctgcgc ttgtcggcga cgaattggcc gacgactgac
     9541 ttcttgtcgc cgcgatactc ggtgtacggg ccatccgcga ggccgtcctt gtacgtggcg
     9601 gaggccgcga ccttgccgtc gatgaacttc tgccacgcgc catcgagctt gccgcgcttc
     9661 cagtgctgct cgagcgtcgg cacgccgagc tccgtctgct cgcgccacag gccgtgcttt
     9721 tgatccttcg agtagcgacc ttcgaccacg gtcttgccgc ggatcgtgag ctcctcgtac
     9781 ttgccgtcga tctggccgtt caccatcggc gtcttcgacg cgatcttctg attcggatag
     9841 aacgtcagca tcgtgccggt gccgttcgtg atcgtgaagc gaccgagctc gttgccggtc
     9901 ttcgtgtcgt agtagacgaa gtcgccgtcg ggcttgccgt cggtgaacat gccctggaac
     9961 gacagcttgt cgtcgcccca ttcgctccac aggccgcgct tctggccatc gctgtagtcg
    10021 ccctcgcgct ccttcttgtt gttcttgtcg ttccacaccc acgtgccggt gcgcttaccg
    10081 tgctcgtaca cgccggtcac gcgctgcact ttccggtcgc gccacatcgt gaactgaccg
    10141 tcgagcttgc ccttcgtgtc gtacgcctcg tcgatcaaca gcgtccagaa ctgccagatc
    10201 ttccgcgggc cgcgtcgcac cccgcgcacg aatgactcct cgatgcgcag cgtgttcttc
    10261 gcgccggcga catgcttgcc gtgcagcttg cccgcgaaca tgttcgcgac gacgacgacc
    10321 gcgccgttgt gatcgaagat gcgcgcgagt ccgttcggca cgcctttgct gagcgtgatc
    10381 tcgctgtaga gcggaccgtc gtcgaaccag cgcttctgct tgccggtgcc ttgtttcatc
    10441 gtgtactcgc cgagcacgtt gccgctcggg ccgagttgct tccacacgcc gtcgcgcaag
    10501 cccgccacgt aggtgccggt ctcggcgatc gcgccgccgg gataatggcg ctgccacgca
    10561 ccgtcgagct tgccgtcctt gtagctgcct gcgatctcga tctcttgatc gggaaacagc
    10621 gtgatgaagt tgccgtaccg cacgccatct gcgcgcgcgc agaaccacgt cggatcggga
    10681 tacggcgctg cggtaacgct ctcgccctcc gcgcacgata atttcggagg aggcggtggt
    10741 ggggcgtccg gtacgacaac gcctgcgtcc ggaacgattg gtaacggtcc ttgcgcgatg
    10801 cgcggtgtgg ttggtgcggg gacagctgtc gctacgagtg gcttctccgt cggcgagcca
    10861 catgctgctg cgaacgtcaa gacgaagcac agcgtgtgcg aacactgcac aacaatattg
    10921 tggcacggag cggcggctac cgtgaaacac gcattacgct agtggaatga aggtaacgag
    10981 gttagttgcg ctctctgtac tgatggcagt ggcggcagcg ccagcggtcg aagcgaggcc
    11041 gcgtcccgcc ggtggtggga acttgaagaa gttctccgcg aacaagacgt tcggcgcggg
    11101 tctcgagctc ggctcgccgt ccgggctcac cggcaagtac ttcctctccg attccaacgc
    11161 gctcgatttt ggcctcggct acatctacca ctcgtactac tacggggacg acggcatcca
    11221 tctgtacctc gactacctgt ggcacccggc gtcgctggtg tcggcggagg cgttcgagct
    11281 gccgttcttc atcggcgtcg gcggtcgcta ctgggacttc gactactgcg acggccgtgg
    11341 ctgcatcaat tatggcggct ctgcgatcgg cgtgcgaatc ccgctcggca tcgccttcga
    11401 tttcaatacc gtcccgctcg atatcgcctt gaatatcgtg ccgacgatcg acttcatccg
    11461 cggcgattac tacgaccgcg tcgaccacca ccgagcgcac ttcggcttcg acgtttcgat
    11521 cgcgattcgc tactggttca agtagtggta gggtgcctcc attcggctcc gacctttggc
    11581 ggagactgga ggttcccagt gtcgcatcgt ctgcatctgt tcaaggcgaa gattcatcgc
    11641 gccaccgtca cgcacgctga tctcgattac gaaggcagcg tgaccatctc cggcgagctc
    11701 atggatgccg ccggaatgct cgagcacgag caggtccaca tctggaacgt cacgcgtggc
    11761 acgcggctcg tcacgtacac gctgcgcggc gacaacgaca gcggcatcgt ctgcatcaac
    11821 ggcgccgccg cgcatctcat gaagcccggc gacaaggtca tcctcgcgac gttcgccgag
    11881 gtcgatgccg cggatgccgc gagctggaag ccgatggtcg tgttcgtcga tgacgcgaac
    11941 aagatcgtcg gtgaaggcgc cgaggtcgcg ggaccgaagc gccggctcac cgcgtaggca
    12001 cgatcgcgct cgcgtgcttc accgcgtcgt ctcgacgacg agcggcacga tcacgccttg
    12061 cgcgtgctcg acttcgatgc cgctgcgccg gacctcgatc tggccgtggc caccgacgct
    12121 gtaatacgcg ctgacgacgt acgtgccggg ctcgaccccg tcgaagcgat aacggccttg
    12181 atcgtcggtg acggtttgca gcacctcgcc gcgcgggccg ccaaccaccg tgacgaccgc
    12241 acctgcgacg cgctcgtggg tgttcatctc gctcaccgcg ccctcgatga ctgcgatctc
    12301 ctgcttcgac ttgtagtgcg tgatcgcgtt ctgcggatcg gcaaagtcga tcgtgaccgg
    12361 agacgtcgcg ccgagcgtga tcttgacgtc gacgtacgtc gcttcgccgc gagtgacgtc
    12421 gatgttcttg atcgtgatcg gctggccggc atattcggcg aggagcgtgt agcgaccggg
    12481 cgttaggtcg tcgagcgtgt acatgccggc tttattcgat tcggtggtca gcccgttcga
    12541 cagccgcagc gatgcgtgct cgagcccctc gccactcgcc acgtcgcgca cgagaccggt
    12601 gatcgcgcct gtcgtcggtc gcttcggcgt gtgcgtagcg ccgcaagctg caagcaatac
    12661 cagcaacatc ctcacgcggg cagtctacgc aaacggggcg ggaggccaag cccggcgacc
    12721 ctggcagtgt ccaactgggt gatgacgggt gcatcacctc ggtcacgtgg caatatcgac
    12781 cgcatgctgc gtgcggcgct actgggactc ctcgcggtgt cggctgtttc ctgtaaatcg
    12841 ggcggcgatg cgccgaaatc acagcctggt gtcgcggccg gcaaggtcgt cgaggtgacc
    12901 ggtaccgtca ccgtgcgaca caacgatgtc gcacagccgc tcgccaaagg cgcgacggta
    12961 gagggcgacg acatcatcga gaccggggcc gacggcaacg cgatcatcga gctgtcgcac
    13021 aacctcgcgc gctgggagct cgggccgaac aagacgtcga aggttcgcga atcgaccgcg
    13081 tggggcttgg cgaagaagtc cggcgccgtc gcggtcgtcc agcaggacac ggcagccgca
    13141 ggtcggcccg ccgagcgcag cgccgccgat acatcggtca gcgcacgcgc cgatgaagcg
    13201 gcacctgcgc ccggtgcggc tccggcgccc gccgccgctg caccgccggc acccgaagcc
    13261 gcgccagctg cagcggttgc tccgccgcct ccgccacccg cgccggagaa ggcggcggca
    13321 ccaacgcgcc agcgctcggt ccgcggtgac gatggtctgc tcgacaagga gagcgggggt
    13381 ggcggcgcgc cgcctgcgcc gccgaagatc acgacacgcg gtggtgtcgc caaggacggc
    13441 gtcggcgccg ggggcggtgg cgcggcgaag gatagtggca gcgtcggtgc aacggcgaat
    13501 atcaccgcgg atgcagcaaa gccgatgaca cctgcggaag ccgacaaggc ggcgacggac
    13561 gtcgtgctca agaacgagaa ggcgctcaag gcatgcctca cgaaggacgc gcagagcgtg
    13621 aacgtgacga tcaaggtcgc cgcgaacggc acggcgacgg cggttgtctc gggcaaggcc
    13681 gagattccgg cggcagtgca gcgctgcgtg aaggccgcgg tcggcaagat ggcgttcgcg
    13741 aaggccgcga cgaccgtctc tatggaaatc tcgcgctgag cgagcgcgct ctcgacggct
    13801 cgctagctcg tgcgtaccga gtcgggcgcg aggccgatct ctgcgcacag cgcggcaagc
    13861 gcctcgcgat tcggaccgcg ccaggcgagc gcgtcgaggt cgcacacgat cggacagtcg
    13921 gtgcggagcg tcgcgagctt ctgatagagc atcgcgagct cgcgcgaatc gcgcagcgac
    13981 gtcgcgaggc gatctgcacc gcgcaggccg gcgacgttcc acttcgcggc gtcatcggga
    14041 atgtgctgga tgtgcttcca caccgtgagc accttcgcag ccgagctctt gccccagccc
    14101 tcgagcccgg ggatgccgtc ggcggtgtca ccgacgagac cgagcagatc ggggatcgat
    14161 tccggcggca cgccgtgctt cgcgatgacg ccgggctcgt cgaggatcgc atccttgtag
    14221 cggtcccatg cgacgacgcg cgtgccgatg acggactgac acaggtcctt gtcgacggcg
    14281 acgatgcgca cctgttcgac gcgcgagtcc ttcgcgaggt gcgcggcggc ggtcgcgatc
    14341 gcgtcgtcgg cttcgaactc gatcatccgc caagcggtga ggccgagtgc ctcggcggcg
    14401 cgctcgacga gcgggaactg tgcgacgagg ttcggttcga ggccctcgcc ggtcttgtag
    14461 ccaaggaata gatcattgcg gaacgactcg atcacggtgt cgaacgcgac gccaccgtgc
    14521 gtgaccgctc ccgagcgcag cacgcgcgcg aggctctgca cgaacgcgcg tgttgcgccg
    14581 acctcacggc cttcgtgctg ggcgggtggc gcgccgaacc acgctcggaa gagctcgtag
    14641 gttccgtcga agagatgggc tcgcacgcga gcaccgtagt acaacagggt gatgtcggcc
    14701 gctagctcgc cactcgccgc agatgcggtc gccggcattc gggacgcccg gctgcgcgat
    14761 atcgtcgccg atcattggga gtacatgatg aagtgggcgc cgacctgggc gaccacgctc
    14821 ggcgaccatc gctacgatga tcggctcgcg ccgcgcgatg ccgccgcgat cgccgagatg
    14881 gatgccgagc acgcagcgtt gctcgcgcgg atcagcgata tcgacatcgc gacgctcggc
    14941 gacaccgacg cggtgacgtg caagctgttg cgcggccggc tcgaagcaga gcaggcactc
    15001 gcgcagctca agcttcacga gcaccacctc gtcgacgccg gcatgagcac ggtgttcgac
    15061 gagctgagct acctgatcga gatgcacgtc gtgcgctcgc acgcggatgc gcagaacgtg
    15121 gtcgcgcgca tgcggatggg tgcgaagctg ttcgacgaca aagtcgcgca cctcgcggtc
    15181 ggtctcgcga cgcgccgggt gtcgtcggcg gaaaagattc gtcgcgtcat cggtcagctc
    15241 gatgcggagc tcgagaagcc cgtcgagacg tgggcgatgg catcgccggc gtgggccgat
    15301 cgcgagcccg agctacacgc ggagctgcgc agcatcatcg cgaccgagat ccaccccgcg
    15361 gtggtgcgca tgcgtgactt cctgcgcgac cgcctgctgc cgaacgcgcg cggcgacaac
    15421 gagggtttgt gcgcgctctc cgacggcgat gcggcctatc gcgcttcgat tctctaccat
    15481 gtcggcctcc cgatcgatcc gcgcacgttg cacgagctcg gcctccgcga gatcgcgcgc
    15541 accgatcgcg agctgaccga gctgggacgc cgcgtgttcg gcacgtcgga tctggcgtcg
    15601 acgttcgcgc ggttgcgcga cgatcgcgcg ctgtacttcg gctccggcgc ggagctgctc
    15661 acggcggcgc agttcgcgct cgatcgtgcg aaggcagcgg tgccgagtta cttctcggtg
    15721 ctgccgatca ccgactgcgt gatgcgcgag attccggact acgaggcgcc gtactcgaca
    15781 atcgcgtact accgccagcc gcactacgac ggcacgaagc cgggcgagta cttcgtcaac
    15841 acgtacaaac cggagacgcg gccgcgctac gagctcgagg cgctgacctg gcacgaatcg
    15901 attccgggcc atcacctgca gatcgcgctc gcgcaagagc tcggcgagct gcccgcgttt
    15961 cgcaagctcg acggttcgac cgcgttcgtc gagggctggg cgctgtacac cgagcacctc
    16021 gccgaggaga tgggcctcta cacggccgac ctcgatcgca tcggtcgcgc gagctatgac
    16081 gcgtggcgcg cgtcgcggct cgtcgtcgac actggcatcc atgcgctcgg ctggacgcgc
    16141 gcgcaagccg aggcgttcat gcgcgagcac accgcgctca cgccgatcaa catctcgaac
    16201 gaggtcgatc gctacatcgg ctggccgggc caggcgctgt cgtacaagtt cgggcagctc
    16261 gagatcatca agctgcgcgg cgaggccgag cagaaactcg gcgcgcgctt cgacctcaaa
    16321 gcatttcatg cgatcgtgct cggcgcgggg gctgtcacgc tacccgttct tgccgatcgc
    16381 gtgaacgcat ggatcaccgc caaccagagc taacccgcgg cctcggcccg tgggctgcgg
    16441 cgtcgctcgt cgtcggcacg atcatcggga ccggcatctt cttgaagacg tcggtgatgg
    16501 ccgcgaccgc cggctccgcg gtgtgggtgc tcgctgcgtg ggcatgcgcc ggtggcctgt
    16561 cgctgtttgg cgctttgacg tacgcggagc tcggcgcgat gtttccgcaa gccggcggcg
    16621 agtacgtgtt cctgcgccac ggctacggcc cgggcatggg ctacctgtac gcgtggaacc
    16681 ggttctggat cgcgacgccg ggctcgatcg cggcatacgc ggtcggcacg acgacattcc
    16741 tgggcggcgt cgtcgacatc gatccggata gcaagctcat cgcgatcggc atgatcgtgc
    16801 tgttcacgct cgcgaactgt ctgcgcgtgc ggaccggcgg cgcgctgcag accgtgctca
    16861 cggcgatgaa ggtcgtgatg atcggcgggc tcgcgttcgg tgcgctgttc ttcgcccgcg
    16921 gcggcgactg gtcgcgcgtt ggcgacagcg ggccgggctt tcccggttgg tcgccattcg
    16981 gcgcgatggt gcttgccgcg ctgtgggcct acgacggctg gaacaacctg ccgatggcgg
    17041 ccggtgaagt ccgcaatccg cagcgcacgt tgccgcgcgc gatcgtgtgg ggctcgctcg
    17101 gtgtcgtcgc gatctacgcg ctcgtcaaca tcggctactt ccacgcgctg ccgtttaccg
    17161 acgtcgccgc gaagagctcg acgtcgatcg cggagcggac cgcgacgacg ttcctcggtg
    17221 tcccggcgca ggcgctgctc atggccgcga tggcgatctc ggcgatttct gcgatgaacg
    17281 gctcgatgct gaccggtgca cgcgtgccgt atgccgtcgc acgcgatggt ctcgcaccaa
    17341 aatcgctcgc ccacgtcgca gagggtgcgc gcgtgccggt cgtcgcggtg ctcgtccaag
    17401 gcgggctcgg cgtgttgtac gcgctgagcg gcaagttcga cgagctcacc gacgcggtcg
    17461 tgttcgcatc ctggctgttc tatggcctca acgcgggcac cgtgctgtta ctgcgcatcc
    17521 gcgaaccgga tcgcgagcgg tcgtatcgtg tgcccggctt tccggtggtg cccgtgatct
    17581 tcatggtgct cgcgctgctg ctgctcgtga acgcgatcgt cacctcgccg cgcgcgagcg
    17641 cactcggtct cgtgatgacc gcgctcggtg gcatcgtcta caccggctac taccggccgc
    17701 gcagcaaggc ttcgagctcg tcctgagccg gcggtgcgcg gtgctcgcgc tggaagttcg
    17761 cgagctcggc gccgccgtga tcgaacacga gcacggtggg aatcttctgc ggccggccga
    17821 ccgccgtgaa cagctcgtcg cccgcgggaa tcacgcgtag ccatggcacc gtgcgggcga
    17881 gcgcgcggat cgcctcggca ttgccgcgct ggtcgtactc ctcgtgcagc ttgtaattga
    17941 ccgcgagcca gcgcacgtgc ggatgggcta cacgtacgcg atcgaacatc gcgatctcgt
    18001 cgcggcaatg gctgcaccac gacgccatca ccacgacgac ggtcgccgac tcgctgcccg
    18061 cgccgacgac ctcgccatcg agatcgcgac tcgcttcgag ttgcgcaaag gtcgagacca
    18121 ctcccggtgt cctcgccgaa ggcgccgatg gcacctcggt gacagtcgcg ttacgcgcgc
    18181 aggaagccgc caagagcgtg atgagtaagg cgcgcacggc cccagaatta tgcaccgcgt
    18241 tgccgattcg gcattgcgaa ccatggcacc cgtcatcaca ttcgtggcat cgaagtatca
    18301 ggaggacgga ttggaaacct tgtacctggg cgcggtggca ttcggagtga cgctcttagc
    18361 agcgtcgttc ttgctcggcg gcaaggacac cgaccacggc gatggtggcc atgcggacat
    18421 gggtctggca tgggcgccgt ttgcgtcgct caggttctgg atcttcctgt tcacgtttgg
    18481 cggtggagcg ggctatgcgc tgt
//
LOCUS       DASUFX010000452         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1740817, whole genome shotgun sequence.
ACCESSION   DASUFX010000452 DASUFX010000000
VERSION     DASUFX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740817"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..2184
                     /locus_tag="VFV99_18245"
     CDS             181..2184
                     /locus_tag="VFV99_18245"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5061317.1"
                     /translation="MRLLRTFAVGLVTTGLAACAGENAGDEELGSAEQAVTSASDSIV
                     SAVTSDPQDIGGFPFAFVEPTTVTVFDLHIVAKSKWQGVVTSNLSWDADKVRQGQTLD
                     VSRSGSALGIMKVLWTVTGTVKPADGLFGTIDIGEIPMDLDVAGCSPPLDGSAFHCEA
                     TSPTITLFDGLLPTTIYVKFGIGVDFDGDGGPASVHRTLYLGDDAKPASDLELTADAQ
                     GDANAMPCDKPVGTTVDYALDPFKWSPSSITVKQQPKIIIGFHDPFLSIPIDAVDIPV
                     GPAIVQHPDFTLEGPGNSVTLGDLQANNVLPTIAPLGPFAGQEGLPVSFSQSTTSACP
                     ITSYVWEFSDGTTSFGPHPQRTFGDDALFNGQLTVTDSTSLSATGSFDVSIGNRPPVA
                     NAGNDTGGAWGTAIALNGQAVDPGADDQATLVYTWDFGDGTPGAGGASVTHAYAVPGD
                     YVATFKACDDHVCDTDTAVVHVRKRTTFVGYTGANAGTYSAPTTLIGSVVDELGLPVV
                     GGTVAFTLGGISSGSAQTNASGNAARTIDVDLPAGPYAVAASYDGSAFYFAGNTAEQF
                     DVSRMSSSLQYTGALGGGPNKTVSLSAKLVDGLNRPLAGQLVTFQLGTQSVSATTAAT
                     GIATASLKLAQKNASYPLTTTWPGNASQWTGAATSVTFSIGGK"
     gene            2590..>4182
                     /locus_tag="VFV99_18250"
     CDS             2590..>4182
                     /locus_tag="VFV99_18250"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5061318.1"
                     /translation="MRSDNIKRGPSRAPARAMLKGAGFTDADLERPLVGIANTWTEVT
                     PCNVHLRALAESVKAGVRAAGGTPIEFNTIAVSDGITMGTEGMRGSLVSREVIADSIE
                     LFTMSHMLDGVVALSGCDKTIPATVMALARMNVPGLVLYGGPIAPGRFEDKDVTIQDV
                     FEAVGAHAAGSMSSTRLAVLEDKACPGAGACGGQFTANTMSVALTLLGMSPMGANEVA
                     ATDPAKHDVARKCGELVMKLIANDVRPRALLTRAAFENAIASVAATAGSTNAVLHLLA
                     IAYEAGVPLAIDDFDAIAARTPVLADLKPGGRFTAVDMTHAGGVRLMVKRMLDAGLVA
                     DQQTCTGKTLREECEGATGDGAPVLRPVSDPLKPRGGFAILRGSLAPEGCVVKLAGHD
                     RQTHTGPARVFDGEEAAFAAVQASAIKPGDVVVIRHEGPRGGPGMREMLAVTAALIGQ
                     GLGDSIALVTDGRFSGATHGLMAGHVSPEAADDGPIAYVRDGDVITFDVAARRLDVDA
                     DLAVRRRVHPAAPRAHGYTHGVI"
BASE COUNT          719 a         1375 c         1375 g          713 t
ORIGIN      
        1 gtacggcttg cggcgcgtcg acgatcgcta ggcaatcgtt gccgaagcaa tcgttgcttc
       61 gagcaagact tgcttcgagt gcggcacgct aagtggccgc gctcactgct ctcactcgtg
      121 taggtgcgaa cggcacatca catgctggag gacgcgtctc cacccagcat gaggaacaac
      181 atgagactac ttcgaacgtt cgcagtcggt ttggtaacaa caggactggc agcgtgtgcc
      241 ggcgaaaacg cgggagacga agagctcggc tcggccgagc aagccgtgac gagcgcgtct
      301 gactcgatcg tctcggcagt caccagcgac ccacaggata tcggcggatt tccgttcgcg
      361 ttcgtcgagc cgacgacggt gacggtcttc gacctgcaca tcgttgcgaa gtcgaagtgg
      421 caaggcgtgg tcacgagcaa cctgtcgtgg gatgccgaca aggtccggca gggccagact
      481 ctcgacgtct cgcgtagcgg ttcggcgctc ggcatcatga aggtgctgtg gacggtgacc
      541 ggcacggtca aaccggccga cggtctcttc ggcacgatcg acatcggcga gatcccgatg
      601 gatctcgacg tcgccggctg ctcgccgccg ctcgatggct cggcgttcca ctgcgaagcg
      661 acctcgccaa cgatcacgtt gttcgacggc ctcttgccga cgacgatcta cgtcaagttc
      721 ggcatcggtg tcgacttcga cggcgacggc ggccccgcct cggtgcatcg cacgctctat
      781 ctgggcgacg acgcgaagcc ggcgagcgac ctcgagctga cggccgatgc acagggcgac
      841 gcgaacgcga tgccgtgcga caagccggtc ggcacgacgg tcgactacgc gctcgatccg
      901 ttcaagtggt cgccgtcgtc gatcacggtc aagcagcagc cgaagatcat catcggcttc
      961 cacgatccgt tcctctcgat cccgatcgac gcggtcgaca tcccggtcgg cccggcgatc
     1021 gtgcagcatc ctgacttcac gctcgaaggt ccgggcaact cggtcacgct cggcgacctg
     1081 caagcgaaca acgtgctgcc gacgatcgcg ccgctcggtc cgttcgcggg ccaggaaggt
     1141 ctgccggttt cgttctcgca gagcacgacg tcggcgtgcc cgatcacgtc gtacgtgtgg
     1201 gagttctcgg acggcacgac gtcgttcggg ccgcacccgc agcgcacgtt cggcgatgac
     1261 gcgctgttca acggtcagct cacggtgacg gactcgacga gcctgtcggc gaccggcagc
     1321 ttcgacgttt cgatcggtaa ccgtccgccg gtcgcgaacg ccggtaacga caccggtggc
     1381 gcgtggggca cggcgatcgc gctcaatggt caggcggtcg atccgggcgc cgatgatcaa
     1441 gcgacgctcg tctacacgtg ggacttcggc gacggtacgc cgggcgccgg cggcgcgagc
     1501 gtgacgcacg cgtatgcggt acccggtgac tacgtcgcca cgttcaaagc gtgtgacgat
     1561 cacgtttgcg ataccgatac ggctgtcgtc cacgttcgca agcgcacgac gtttgtcggc
     1621 tacaccggcg cgaacgccgg cacgtactcg gcaccgacca cgctgatcgg ctcggtcgtc
     1681 gacgagctcg gcctgccggt cgtcggcggc accgtagcct tcacgctcgg cggaatcagt
     1741 tccggcagtg cgcagacgaa cgcgtcgggc aacgccgcac gcaccatcga cgtggatttg
     1801 cccgcgggcc cctacgcggt cgcggcgtcg tacgacggtt cggcttttta ctttgccggc
     1861 aacacggcgg agcagttcga cgtgtcgcgc atgtcatcgt cgctgcagta caccggcgca
     1921 ttgggcggag gcccgaacaa gaccgtctcg ctcagtgcca agctcgtcga cggcttgaac
     1981 cgtccgctcg ctggccagct cgtcacgttc cagctcggca cgcagagcgt gtcggcgacg
     2041 acggcggcga ccggcatcgc gaccgcatcg ctcaagctcg cgcagaagaa cgccagctac
     2101 ccgctgacga cgacgtggcc gggaaatgcg agccagtgga cgggcgccgc gacctcggtg
     2161 accttctcga tcggtggcaa gtagggtact gaatccaaga accgaccggt cggtaggtgt
     2221 gaaagtcatt ttttctcacc taccgattcg gttttagata agatgactta tcccgtgttg
     2281 cagaccccgc ctacattgac gcacagcaac aagcgagttg acgcgctgcg ctactcgcgc
     2341 gtagagtgtg tgtcgatgat gggtaccacg aagatgacgc aggcgacttc gccggtcgtc
     2401 tcttcggtcg gagctctcgc gcgctcgccg ctcgcgattc tgacgattct cattatcggc
     2461 ggcgtactcc tccttattac gggcggcggg cccccgattt aaccggacaa cacagtcccc
     2521 aaatcggccc ccgccctgga aacggagcgg gggctttttc gttccccctt ttctcaacga
     2581 gtcgattcga tgcgcagtga caacatcaaa cgcggtcctt cccgagcacc cgcacgcgcg
     2641 atgctcaagg gcgccggctt caccgacgct gatctcgaac gcccgctggt cggcatcgca
     2701 aatacctgga ccgaggtcac gccgtgcaac gtgcatctgc gcgcgctcgc cgaatcggtg
     2761 aaagcaggcg tgcgcgcggc cggcggcaca ccgatcgagt tcaacacgat cgcggtgagc
     2821 gacggcatca cgatgggcac cgaaggcatg cggggctcgc tcgtgtcgcg cgaagtgatc
     2881 gccgattcga tcgagctgtt cacgatgtcg cacatgctcg acggcgtcgt cgcgctgtcc
     2941 ggttgcgaca agacgattcc ggcaacggtg atggcgctcg cgcgcatgaa tgtgccgggc
     3001 ctcgtgctct acggcggtcc gatcgcgccg ggtcgtttcg aagacaagga cgtcacgatc
     3061 caggacgtat tcgaagcagt cggcgcgcac gccgctggct cgatgtcgtc gacgcggctc
     3121 gccgtgctcg aggacaaagc gtgtcccggc gccggtgcat gcggcggtca gttcaccgcg
     3181 aatacgatga gtgtcgcgct cacgctgctc ggcatgtcgc caatgggcgc gaacgaggtt
     3241 gccgcgacag atcccgcgaa gcacgacgtc gcccggaagt gcggtgagct cgtgatgaag
     3301 ctgatcgcga acgacgtgcg accgcgcgcg ctgctgacgc gcgctgcgtt cgagaacgcg
     3361 atcgcatcgg tcgccgcgac ggcaggctcg acgaatgcgg tgctgcacct gctcgcgatc
     3421 gcctacgaag caggcgtccc gctcgcgatc gatgacttcg acgcgatcgc ggcgcgcacc
     3481 ccggtgctcg ccgatctgaa gccgggcggc agattcaccg ccgtcgacat gacgcacgcc
     3541 ggtggcgtgc gcctgatggt gaaacgcatg ctcgatgccg gtctggtcgc tgaccaacaa
     3601 acgtgtaccg gcaagacact ccgcgaagaa tgcgaggggg cgaccggtga cggcgccccc
     3661 gttttgcgtc cggtgtcgga tccgctgaag ccgcgcggtg gcttcgcgat cctgcgcggc
     3721 tcgctcgcgc ccgaaggctg tgtcgtcaag ctcgccggtc acgaccgcca aacgcatacc
     3781 ggtccggcgc gcgtgttcga cggtgaagaa gccgcgttcg ccgcggtgca ggctagcgcg
     3841 atcaagccgg gcgacgtcgt cgtgatccgc cacgaaggtc cgcgcggcgg gccaggcatg
     3901 cgcgagatgc tcgccgtcac cgcggcgctg atcggccaag gcctcggcga ttcgatcgcg
     3961 ctcgtcaccg acggcaggtt cagcggcgcg acgcacggtc tgatggccgg tcacgtctcg
     4021 cccgaagccg ccgacgacgg accgatcgcg tacgtgcgcg acggtgatgt gatcacgttc
     4081 gacgtcgccg cacgccggct cgatgtcgac gctgatctcg cagtgcgcag acgcgtccac
     4141 ccggccgcgc cgcgcgcgca tggctacacg cacggcgtga tc
//
LOCUS       DASUFX010000453        10801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1602298, whole genome shotgun sequence.
ACCESSION   DASUFX010000453 DASUFX010000000
VERSION     DASUFX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602298"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFV99_18255"
     CDS             complement(<1..500)
                     /locus_tag="VFV99_18255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEX5061319.1"
                     /translation="MRHIRLVVEYDGSHLHGWQRQDNAPTVQQHLEEALAKLLTHETQ
                     VVGASRTDAGVHARGQVASFRTERPIPVHGIRRGLNSMLPDTIAVRDASEVPDDFHPR
                     FSATGKHYRYTILTRNERSPRFRDRSWHHPEPLDVRAMQVSLARDRREARGTRLIGLH
                     ARDVKI"
     gene            complement(515..3094)
                     /locus_tag="VFV99_18260"
     CDS             complement(515..3094)
                     /locus_tag="VFV99_18260"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5061320.1"
                     /translation="MVRPAWLVALAVAIAAPARASSVLPGRMGMYTQQGAALAMTDSK
                     IVVRVRGPIAEAVVTQTFKNDLDRVTEATYIFPLPVDAAVSAMEIESGARTIHAAIEV
                     REQAQQRYETAVAAGVGAALLDQERPDVFTQTVSAIPAKGTVVVTLRFDTVARYHGGH
                     WELVLPMVVAPRYVPGSANGLPTTGTGRAPDTDRAPDASRVTPGGAPGAGGKTDVVLE
                     LGNDVDDVSSPTHDLVKEKAGYALHDAKSDHDAVIRWRAKVPAAGWVEASDDGGFAAV
                     VVEARAPAAKKSPLRMMLVLERAATTRGDADNVKHPLVRALLGALDSKDQVSLAGSDK
                     LDWRAPDAVLRVLEDSWHKAGGPFDLTKTLQQLRSNGAPLVLVTDGLVADDRAVLVAA
                     AKVGAPIHVIGIGPAPNRGLLTQLAATTGGTVRFATIGDDTSAIAKDVLADAASQPEP
                     LSITWGTLAASDVVPATLPRLGAGQAMLVVAKVKKVQTANARVRGDLFGFTMVTVPKP
                     PDGATTPKGSLARRWAKLKLDDLVAGGNAKTITEHALHYGLVSPYTSMVAIGDEVVVK
                     GGVKHSVPVTVSVPEGMRWQLVKREITIDTRIDTTVTKQEQTKLAEDKKKPKDKLPAK
                     KEPGRDEHAQKPRDTKPRTERPQPVAVPEATAVPTQPTIDHAGAEGAGATNEDDGDYE
                     GRKSKRDADEEAPRAEPAPPSPGSVATADYDADGEMESLSISSSRYKQRRLTLSLGGG
                     VSVVNGDAAPQLGLALRLDWGRETRIGGEASVWLVDGLHGQGNVLATVAQRIARRLEL
                     GIGGGLRFTGDVAGPALNLTLRSHLPIRGLSTFLRYDGALLIHDGTYDGQNGGSFGFE
                     ASF"
     gene            complement(3185..3661)
                     /locus_tag="VFV99_18265"
     CDS             complement(3185..3661)
                     /locus_tag="VFV99_18265"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEX5061321.1"
                     /translation="MLVIIHSGQTGVERGAHRAAVSAGLSVAGFMPLDRRDELGPIPA
                     DVAQLLTPCFDRGPRPAVRANITLASGVLLVVPESGTPEKFTAMSAVMQGIRSARVPS
                     MVVDGKTNLDDVVRWARQLPETSGSTRLMVTGPRGTRWPDGETVSRRLVAAIGITQ"
     gene            4023..5756
                     /locus_tag="VFV99_18270"
     CDS             4023..5756
                     /locus_tag="VFV99_18270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX5061322.1"
                     /translation="MFTSGETEWCENLRMLVARQVPSEETYVTIALAPILDDAGTIEG
                     VAGACLDVTAKVIADRRLALLHKIAVGLAAVRSLDVAAKTLTHILRNAPDVAFATLYA
                     VDGGVAQARVWIGTNTETSGLPAIADRGSILGPVLETEEAIEVTIGDGSQRALALPVR
                     APGDRLAGILVCGLNPLAPLDAMYRAFLETIAQNVGATFVDAGAVRGVATPAPQLRTT
                     DAFLAVLGHELRNPLGALMTTLQTMMLRAPSLEVELMERSVRTLTRMVDNVLDVSRIA
                     RGGLELRPTPTELATIVDRAMQLVTPLVTERNTQVFVQIPRTGLRCNVDSERVAQAIA
                     GLLSNASRYSDPGSRVWVQAAREYDRARIIIKDEGSGIEPDRLAEIFGAAYVATEKRP
                     RNAGLGLGLVIARSLVELHGGSLNVASPGMGRGTEAVIELPIDARIATSGPVANAPGS
                     RRRLLLVEDNDDAARSLKNALELLGYVVALAHNGPIALNVARTFDPDVVLLDIGLPVM
                     DGWELAKRLRELAGATHDAPVVAVTAYDRDIDKQRSTDAGFADHLVKPIDLGKLQEVV
                     ESLPMRPSPAS"
     gene            complement(5787..6605)
                     /locus_tag="VFV99_18275"
     CDS             complement(5787..6605)
                     /locus_tag="VFV99_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061323.1"
                     /translation="MRMAIFAVLAACTGGGGSSMLDPDAATTSDTTPEVDAYTIIDQD
                     NDGLDDLREQQLAEDYLPFISMDPGDGCPLSGLVARVRPHPADPAKVLIVYSHLFQRD
                     CGLAGHIGDNEAFGIAIDPNLPAPTGILAIRTASHQNTPCERISDCTTCAGDSRPQCD
                     VTIANGAQWPVLYASKDKHGQYATKSKCGFGTCFDQCTLNAMAQLPPITNVGEPGHAF
                     THDLTANGFITPANGWTEAELMNFDPWDATKDFGGAGNIAGDLQDSTFEPALCN"
     gene            complement(6658..7464)
                     /locus_tag="VFV99_18280"
     CDS             complement(6658..7464)
                     /locus_tag="VFV99_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061324.1"
                     /translation="MVWRLIGGLASGVAFLVGRRAYRKKGQESAARAAGLEPTDDLTH
                     VPRALQQTALWSLADGGFERRVMHGKVARTGGDVSITAFDLETLRERRGEWAFLPVEP
                     PFRIGGVVSVVVCEVDRRFPHVLFKHAGRGDELLDDELLDRLIHVAKAARDRLGMKRA
                     YPSELPGTLANQPVSASLPPHWRAYTRTPELVEMLAAAGLRTVLEQASRRDLVIELLE
                     RVVVVYPAARDVVGPDAFADLTATALQIVDGVLAASPPLTPRGIEHVTTS"
     gene            complement(7484..7984)
                     /locus_tag="VFV99_18285"
     CDS             complement(7484..7984)
                     /locus_tag="VFV99_18285"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5061325.1"
                     /translation="MNMLTFEAPFGVLRLYGHADELCALHLPNSPDSDDAPDVPEGRS
                     RVLEATARQLTEYFAGTRRDFDLPLAPHGTAFQQLVWRALLKIPFGATCSYGHIARAI
                     RRPSASRAVGAANGKNPIAIIVPCHRVIGSTGDLTGYGGGLPTKRWLLAHEARYTPAT
                     QTSLRI"
     gene            complement(7987..9423)
                     /locus_tag="VFV99_18290"
     CDS             complement(7987..9423)
                     /locus_tag="VFV99_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX5061326.1"
                     /translation="MLTDDARYQAMCARDSRFDGVFFVGVRTTGIYCRPICPARTPGR
                     NRCEFFATPALAERAGFRACFRCRPELAPGNASVDAVDALVAGATQRIAEGALNDGSL
                     DELAGQLGVSARHLRRALEAKLGVSPVELAQSRRLALAKQLLQDTGLSLTEIAFAAGF
                     GSVRRFNAVFAEKMGSSPSALRRKHVDAVAGDAVTLRLDYRAPYDWERLVGFLRGRAI
                     PGVELVGDGSYRRVVHLGGETGELEVTQARSRDALQLSVSPALLPVLMPLVARVRRMF
                     DLDARPDLINAALRRDKTLGPLIAKRPGLRVPGAIDPFEASIRALLGQQVSVAAATTL
                     AGRFATQLGAPFAGSAGLTHRFPTAAEVVAAGAARIGKLGMPQARGTAIHTFAREVAS
                     GTLRLDAACELDAFVDQLVALPGIGPWTAHYLAMRALHVPDAFPAADLGVQKALKRAG
                     AKAAEARAEAWRPFRAYAVLHLWTGLGD"
     gene            complement(9504..10052)
                     /locus_tag="VFV99_18295"
     CDS             complement(9504..10052)
                     /locus_tag="VFV99_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061327.1"
                     /translation="MRGDIAFVGFMMTAEEWQELDPESRAQLIAVATRRADPDVPQSA
                     PRRFPDGTGKHEIVESIDDELESLIELDLLDSSLLEPLDAADAAIEAEIARVDRDGDI
                     ALFTDDEIAFDMTAQFDALDEWLALQPQYECVDHDEWVTLPEPVSPVPLPAPACDTTL
                     PEPVVAAVITEPFEDFADWADL"
     gene            10113..>10801
                     /locus_tag="VFV99_18300"
     CDS             10113..>10801
                     /locus_tag="VFV99_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061328.1"
                     /translation="MPRVVTYRYIDPLTQVWLGTAQRIGLRVIRTPDAYAATDGSGTL
                     AIGDDTALDPDDSLAQMIFHELCHSLVEGEESFTRADWGMDNTGPDHDWREHACLRVQ
                     WVLTGRHGLRTLFAPTTDFRASFWDRLSDDVLSDRTDPSVQAAILGLRRAALPPWAPH
                     LAEALAATARIAQEAAKLATEPTVLWHGVTPAPAAHPTGLPAGAASGTCGGCAWRFEQ
                     RGVAKCRHTNT"
BASE COUNT         1723 a         3692 c         3601 g         1785 t
ORIGIN      
        1 tcgatcttca cgtcgcgcgc atgaaggccg atgaggcgag tccctcgagc ctcgcgacga
       61 tcacgcgcga gcgagacctg catcgcgcgg acatccagcg gctcggggtg atgccatgac
      121 cgatcgcgga agcgcggtga gcgttcgttg cgcgtcagga tcgtgtagcg gtagtgcttg
      181 ccggtcgccg agaagcgcgg atgaaagtcg tccggcacct cgctcgcgtc gcgcaccgcg
      241 atcgtgtcgg gaagcatgct gttgagcccg cggcgaatgc cgtgcacggg aatcggccgc
      301 tcggtgcgaa agctcgcgac ctgcccgcgc gcgtgcacgc cggcatccgt gcgcgacgcg
      361 ccgaccactt gcgtctcgtg cgtgagcagc ttcgcgagcg cttcctcgag atgctgctgc
      421 acggtcggcg cgttgtcttg ccgctgccag ccgtggagat gactgccgtc gtactcgacg
      481 acgagccgga tgtgacgcac gaggcgagct tactttagaa gctcgcctcg aagccaaacg
      541 agccgccgtt ctgaccgtcg tacgtgccgt cgtggatcag cagcgcgccg tcgtagcgca
      601 ggaacgtgct gaggccgcga atcggcaggt gcgagcgcag cgtcaggttg agcgccggtc
      661 ccgccacatc gcccgtgaag cgcagaccgc cgccgatgcc gagctcgagc cggcgcgcga
      721 tgcgctgcgc gaccgtcgcg agcacgttgc cctggccgtg gagaccgtcg acgagccaca
      781 ccgatgcctc gccgccgatc cgcgtctcgc gcccccagtc gagtcgtagt gcgagcccga
      841 gctgaggcgc cgcgtcgccg ttgacgaccg acacaccgcc gccgagcgat agcgtcagcc
      901 gccgctgctt gtagcgcgac gacgagatcg acaacgactc catctcgcca tcggcgtcgt
      961 agtccgcggt cgcgaccgag cccgggctcg gcggcgcagg ttccgcgcgc ggcgcttcct
     1021 cgtcggcgtc gcgcttgctc ttgcggcctt cgtaatcacc gtcatcctcg ttggtcgcgc
     1081 ctgcgccctc ggcgcccgcg tgatcgatgg tgggttgcgt cggcaccgca gtcgcttcgg
     1141 gtacggcgac cggctgcgga cgttccgtcc gcggcttcgt gtcgcgcggc ttctgcgcgt
     1201 gttcgtcgcg acccggctct ttcttcgccg gaagcttgtc cttcggcttc ttcttgtcct
     1261 cggcgagctt cgtctgctct tgctttgtca cggtcgtatc gatgcgcgtg tcgatcgtga
     1321 tctcgcgctt gaccagctgc cagcgcatgc cttcgggtac cgatacggtg accggcacgc
     1381 tgtgcttgac gccacccttc acgacgacct cgtcgccgat cgcgaccatc gacgtgtacg
     1441 gcgacacgag gccgtagtgc aacgcgtgct cggtgatcgt cttcgcgttc ccaccggcaa
     1501 ccaggtcgtc gagcttcagc ttcgcccacc ggcgtgcaag cgaacccttc ggcgtcgtcg
     1561 cgccatcggg cggcttcggc acggtgacca tcgtgaagcc gaacagatcg ccgcgcacgc
     1621 gcgcgttcgc ggtctggacc ttcttgacct tcgcgacgac gagcatcgct tgcccggcgc
     1681 caaggcgcgg tagcgtcgcc ggcacgacat cgctcgcggc gagcgtgccc cacgtgatcg
     1741 acagcggctc gggctgcgac gccgcatcgg cgagcacgtc ctttgcgatc gcgctcgtgt
     1801 cgtcgccaat cgtcgcgaag cgcaccgtgc ctccggtcgt cgccgcgagc tgcgtgagca
     1861 agccgcgatt cggcgccgga ccgatgccga tcacatgaat cggcgcgccg accttcgccg
     1921 ccgcgacaag caccgcgcga tcgtcggcga caaggccgtc ggtgacgagc acgagcggcg
     1981 cgccgttcga gcgcagctgc tgcagcgtct tcgtcagatc gaacgggccg ccggctttgt
     2041 gccagctgtc ttcgagcacg cgcaacaccg cgtcgggtgc gcgccagtct agcttgtcgc
     2101 tgcccgcgag cgaaacttga tccttgctgt cgagcgcgcc cagcaacgcg cgcacgagcg
     2161 gatgcttcac gttgtcggca tcaccacgcg tcgtcgcggc gcgctcgagc acgagcatca
     2221 ttcgcagcgg cgatttcttc gcagcgggcg cccgcgcttc gacgaccaca gccgcgaatc
     2281 caccgtcgtc gctcgcctcg acccagccgg ccgccggcac cttcgcgcgc cagcgaatca
     2341 cggcatcgtg atcgctcttg gcgtcgtgca gcgcgtagcc agctttctcc ttcacgagat
     2401 cgtgcgtcgg actcgagacg tcatcgacat cgttgccgag ctcgagcacg acgtcggtct
     2461 tgccgcccgc gcctggcgca cctcccggcg tcactcgcga tgcatccggc gcgcgatcgg
     2521 tatcgggcgc gcggccggtg ccggtcgtcg gcaaaccgtt cgcactgccc ggcacgtagc
     2581 gcggcgccac gaccatcggc aacacgagct cccagtgccc gccgtgatag cgcgcgaccg
     2641 tatcgaagcg cagcgtgacg acgaccgtgc ccttcgcggg aatcgccgat acggtctgcg
     2701 tgaacacgtc ggggcgttct tgatcgagga gcgccgcgcc gacgccggcc gcgactgccg
     2761 tctcgtagcg ctgctgcgct tgttcgcgta cttcgatcgc ggcgtgaatc gtccgtgcgc
     2821 cgctctcgat ctccatcgcg gacaccgcag cgtccactgg caacggaaaa atgtaggtcg
     2881 cctcggtcac gcgatcgagg tcgttcttga acgtttgggt cacgaccgct tcggcgatcg
     2941 gcccgcgcac gcgcacgacg atcttgctgt cggtcatcgc gagcgctgcg ccctgctgcg
     3001 tgtacatgcc catgcgtcct ggcagaacgc tggacgcgcg ggcaggagcc gcgatcgcga
     3061 ccgccagcgc aacgagccac gcaggacgca ccatgccttg cgacattaca cgcatggcgc
     3121 gcttggccga tctatagagg cgcgcttcgc gggcctcgcg cctccctcac ctatctgccc
     3181 ggacttactg cgtgatgccg atggcagcta cgagccggcg ggagacggtc tcgccgtcgg
     3241 gccaccgcgt gccacgcgga ccggtcacca tcagccgcgt cgagccgctg gtctctggca
     3301 gctggcgagc ccaacgcacc acgtcgtcga ggttcgtttt gccgtccacg accatcgacg
     3361 gcacgcgtgc cgagcgaatg ccctgcatca cggcgctcat cgcggtgaat ttttccgggg
     3421 ttcccgattc gggcaccacg agaagcacgc ccgacgcgag agtgatgttc gctcggacgg
     3481 ctggacgcgg tccgcgatcg aagcatggcg tgagtagctg cgcgacatca gcgggaattg
     3541 gccctagctc gtctcgtcga tcgagcggca tgaaacccgc tacggaaaga cccgctgaca
     3601 ccgctgcgcg atgtgccccg cgctccacgc ccgtctgtcc tgaatgaatg attaccaaca
     3661 tgaccctgcc cgattgccga ggacactaaa ccatggtttt gtcgctgcac atggacccgg
     3721 atcgtcttcg tcgacaattc ccgggaaata gtcacatcgc aacgttgctg cgacagatcg
     3781 attggacctc caacgacctc ggctcgccgg agacatggtc gcatagcctg caagccgcga
     3841 tcgcgaattc tctggcgtcg ccgacgccca tgttcgtctg gtgggggccc gcgcgcatct
     3901 tcgtgtacaa cgacgcatcg atcgagttgc ttcacagtcg gcacccgagc gcactagcac
     3961 gctccggacg cgatgcctgg agcgacgaag tgtgggctgt cctcggacct acgatcgagc
     4021 gcgtgttcac gtcgggcgag accgagtggt gcgagaacct acggatgctc gtggctcgcc
     4081 aggttccgag cgaggagacc tacgtaacga tcgcgttggc gccgatcctc gacgacgcag
     4141 gtacgatcga gggcgtagct ggcgcgtgcc tggacgtgac cgccaaggtg atcgccgatc
     4201 ggcggctggc gctgcttcac aagatcgcag tcggtctggc cgcggttcgt tcactcgacg
     4261 tcgcagcgaa gacactcacg cacatcctgc gtaacgcacc ggatgtcgcg ttcgcgactc
     4321 tgtatgccgt cgacggtggt gtggcgcagg ctcgcgtctg gatcggtacg aataccgaga
     4381 cgagtggatt gcccgccatc gccgaccgcg gctcgatcct cgggcccgtg ctcgaaaccg
     4441 aggaggcgat cgaggtcacg atcggtgacg gaagtcagcg ggcgctcgcg ctgccggttc
     4501 gtgcaccggg cgaccgcctc gccggcatcc tcgtctgcgg cctcaacccg ctcgcgccgc
     4561 tcgacgctat gtatcgcgcg ttcctcgaga cgatcgccca gaatgtcggc gcgacgttcg
     4621 tcgacgcggg cgccgtgcgc ggcgtagcga cgccggcgcc gcagttgcga acgacagacg
     4681 cattcctcgc ggttctcggt cacgagctgc gcaatccact tggcgcgctc atgacgactc
     4741 tgcagacaat gatgttgcga gcgccatcgc tggaggtcga gctcatggag cggtcggtac
     4801 ggaccctcac gcgcatggtc gacaacgtgc tcgacgtctc gcgcatcgcg cgcggcggcc
     4861 tcgagctgcg accgacacca accgagctcg cgaccatcgt cgatcgtgcg atgcaactcg
     4921 tgaccccgct cgtcaccgag cgcaacacac aggtgttcgt gcagataccg cgcaccggct
     4981 tgcggtgcaa cgtcgattcc gagcgtgtcg ctcaggcgat cgcgggtctg ctttcgaatg
     5041 cgtcgcgcta cagcgatccc ggctcgcgcg tgtgggtcca agcggcgcgc gagtacgatc
     5101 gcgcccgcat catcatcaaa gacgaaggca gcggcatcga gcccgaccgg ctcgcagaga
     5161 tcttcggcgc tgcctacgta gcgaccgaga agcggccgcg caacgctggc ctcggccttg
     5221 gcctcgtgat cgcgcgcagc ctcgtcgagt tgcatggtgg gtcgctgaac gtcgcgagcc
     5281 cgggcatggg ccgcggcacg gaggcggtga tcgagctgcc gatcgacgca cggatcgcaa
     5341 cgtcgggccc ggtcgcgaat gcacccggct cgcggcggcg gctcctgctc gtcgaggaca
     5401 acgacgatgc cgcgcgctcg ctcaagaatg cactcgagct gctcggctac gtcgtcgccc
     5461 tcgcgcacaa cggcccgatc gcgctcaacg tcgcacgtac gttcgatccc gatgtcgtgc
     5521 tcttggatat cggccttccg gtcatggacg gatgggagct cgcgaagcgg ctccgcgagc
     5581 tggcgggcgc gacccacgac gcgccggtcg tcgccgtcac cgcgtacgat cgcgacatcg
     5641 acaagcagcg ctcgacggac gccggctttg cagatcacct ggtcaaaccg atcgatctcg
     5701 gcaagctcca ggaagtggtc gagagcctac cgatgcggcc atcgcctgct tcgtgaccgc
     5761 gacgcgcgcg agcagcagct cggcgactag ttacagagcg caggctcgaa cgtcgagtct
     5821 tgcagatcgc ctgcgatgtt gccggcgccg ccgaagtcct tcgtcgcgtc ccacgggtcg
     5881 aagttcatca gctcggcttc ggtccagccg ttcgccggcg tgatgaagcc gttcgccgtg
     5941 aggtcgtgtg tgaatgcgtg gccgggctcg ccgacgttcg tgatcggtgg cagctgagcc
     6001 atcgcattga gcgtgcactg atcgaagcac gtcccgaacc cgcacttcga tttcgtcgcg
     6061 tactggccgt gcttgtcctt gcttgcatag agcaccggcc actgcgcgcc gttcgcaatc
     6121 gtgacgtcgc attgcgggcg cgaatcgccg gcgcacgtcg tgcagtcgct gatgcgctcg
     6181 cacggcgtgt tctgatgtga cgcggtgcgg atcgcgagga tgccggtggg cgcaggcagg
     6241 tttggatcga tcgcgatgcc gaacgcctcg ttgtcgccaa tgtggccggc gaggccgcag
     6301 tcgcgctgga acaagtgcga gtacacgatc aggaccttcg ccggatcggc ggggtgcggc
     6361 cgcacgcgcg cgacgagtcc ggacagcggg caaccgtcgc cgggatccat cgagatgaac
     6421 ggcaggtagt cttcggcgag ttgttgttcg cgcagatcgt cgaggccgtc gttgtcttga
     6481 tcgatgatcg tgtatgcgtc gacttcgggc gtggtgtcgc tggtcgtcgc cgcgtcagga
     6541 tcgagcatgc tgctgccgcc gccgccagtg cacgcggcga gcacggcgaa aatcgccatc
     6601 ctcatcgccg tgcatcgtat ggggctcagt cgggcgacga aatcgttaga tcacgagtca
     6661 cgaagttgtt acgtgctcga tgccgcgcgg cgttagcggc ggcgaagcag cgagaactcc
     6721 gtcgacgatc tgcagcgcgg tcgccgtgag gtcggcgaac gcatctggcc cgaccacgtc
     6781 acgagcagcc ggatagacga cgacaacgcg ttcgagcagc tcgatcacga ggtcgcgccg
     6841 gctcgcctgt tcgaggacgg tgcgtagccc tgcggcagcg agcatctcga ccagctccgg
     6901 cgttcgcgtg tacgcgcgcc agtgcggagg caacgacgcg ctgactggtt ggttcgcgag
     6961 cgtcccgggc agctccgacg gatacgcgcg cttcatgccg agtcgatcac gcgcggcctt
     7021 cgcgacgtgg atcaaccgat cgaggagctc gtcatcgagt agctcgtcgc cacgccctgc
     7081 atgcttgaac agcacgtgtg gaaaccggcg atcgacttcg cacacgacga cactcacgac
     7141 gccgccgatg cgaaacggtg gctcgaccgg caagaacgcc cactcgcctc gccgctcgcg
     7201 cagcgtctcg agatcgaacg cggtgatcga gacatcacca cccgtgcgcg cgaccttgcc
     7261 atgcatgaca cggcgctcga atccgccgtc cgcgagcgac cacagtgcgg tctgctgcag
     7321 tgcacgtggc acgtgcgtga ggtcgtccgt tggctcgagg cccgccgcgc gcgccgccga
     7381 ctcctgacct ttcttgcgat acgccctgcg tccgacaagg aacgccacgc cgctcgcgag
     7441 tccgccgatc agcctccaga ccacggggcg accgtagcag gcatcagatg cgcaggctcg
     7501 tctgcgtggc gggcgtatag cgcgcttcgt gcgcgagcag ccatcgcttc gtcggcagac
     7561 cgccgccgta gcccgtgaga tcaccagtcg atccgatcac gcgatgacac ggcacgatga
     7621 tcgcgatcgg attcttgccg ttcgcggcgc cgacggctcg gctcgccgac ggacgacgaa
     7681 tcgcgcgtgc gatgtggccg tagctgcagg tcgcgccgaa tggaatcttc agcagcgcgc
     7741 gccacacgag ctgctgaaac gcggtgccat gcggcgcgag cggtagatcg aagtcgcgcc
     7801 gggtgccggc gaagtattcg gtgagctggc gcgccgtcgc ctcgagcacg cggctacggc
     7861 cttccggcac atcaggcgcg tcgtcggagt ccggggaatt cggcaggtgc agcgcgcaga
     7921 gctcgtccgc gtgtccgtag aggcggagca caccaaacgg tgcttcgaac gtgagcatgt
     7981 tcatggtcag tctccgagtc cagtccagag gtgaaggacg gcgtacgcgc gaaacgggcg
     8041 ccacgcttcg gcgcgcgctt ccgcggcttt cgcgccggcg cgcttcagcg ctttctgcac
     8101 gccgaggtcc gcggcgggaa atgcatccgg tacgtgcaat gcacgcatcg cgaggtagtg
     8161 cgcggtccac ggtccgatac cgggaagcgc gacaagctgg tcgacgaacg catcgagctc
     8221 gcacgccgcg tcgagccgca acgtgccgct cgcgacctcg cgcgcgaacg tgtggatagc
     8281 ggtgccacgt gcttgcggca ttccgagctt gccgatgcgc gccgcacccg cggcgacgac
     8341 ctcggcggcg gtcggaaagc gatgcgtgag tcccgcgctg cccgcgaacg gcgcgccgag
     8401 ctgagtggcg aaccgaccgg caagcgtcgt cgctgcggcg accgagacct gctggccgag
     8461 caacgcgcgg atcgacgctt cgaacggatc gatcgcgccc ggcacgcgca accccgggcg
     8521 cttcgcgatc agcggcccga gcgtcttgtc gcggcgcagt gccgcgttga tcagatccgg
     8581 acgcgcatcg agatcgaaca tgcgccgcac gcgcgcgacg agcggcatca gcaccggtag
     8641 cagagccggc gataccgaga gctgcagcgc atcgcgcgag cgcgcttggg tgacctcgag
     8701 ctcgccggtc tcgccgccga gatgcacgac gcgccgatac gagccgtcac cgacgagctc
     8761 gacgccggga atcgcgcgcc cgcgcagaaa cccgaccagc cgctcccagt cgtacggtgc
     8821 gcgatagtcg agccggagcg tcaccgcgtc gcccgcaacc gcatcaacgt gcttgcggcg
     8881 cagagcggac ggcgagctgc ccatcttctc ggcgaacacg gcattgaacc gccgcacgct
     8941 gccgaagccg gctgcgaacg cgatctccgt cagcgacaac ccggtgtctt gcaacagctg
     9001 cttcgcgagt gcgagccggc gactttgcgc gagctcgacc ggcgacacgc cgagctttgc
     9061 ttcgagtgca cgccgcaggt ggcgcgcact gacgccgagc tggcccgcaa gctcgtcgag
     9121 cgagccgtcg ttgagcgcgc cttctgcgat ccgctgcgtc gcgccggcga cgagcgcgtc
     9181 gactgcgtct accgatgcgt tgccaggcgc gagctcgggg cggcagcgaa agcacgcgcg
     9241 aaagccggcg cgctccgcga gcgcgggcgt cgcgaaaaac tcgcagcgat tgcggcccgg
     9301 ggttcgcgcc gggcagatcg gccgacagta gatgccggtc gtgcgcacgc cgacgaagaa
     9361 cacgccgtcg aagcgcgagt cgcgcgcgca catcgcttgg tagcgggcat catcggtcag
     9421 cacgcattcg aggtaccacg cggccctgcc cctggctggc cgtttccgga cacgaccaca
     9481 acttcgggat ctgcccgagg agcctagagg tcagcccagt ccgcgaagtc ctcgaacggt
     9541 tcagtgatta ccgctgccac gaccggctcg ggcagcgtcg tgtcgcacgc gggcgccggt
     9601 agcgggaccg gggacaccgg ttccggcagc gtcacccact cgtcgtggtc gacacattcg
     9661 tactgcggct gcagcgcgag ccactcgtcg agcgcgtcga actgcgcggt catgtcgaac
     9721 gcgatctcgt cgtccgtgaa cagtgcaatg tcgccgtcgc gatcgacgcg tgcgatttcc
     9781 gcctcgatcg cggcatctgc ggcgtcgagc ggctcgagca gcgacgaatc gagcaggtcg
     9841 agctcgatca ggctctcgag ctcgtcatcg atcgattcga cgatttcgtg cttgccggtc
     9901 ccatccggga agcggcgcgg cgcgctctgc ggcacgtcag ggtctgcgcg gcgtgtcgcg
     9961 acggcaatca gctgcgcgcg ggactcggga tcgagctcct gccactcctc ggcagtcatc
    10021 atgaacccga cgaacgcgat gtcacccctc atagacccca cgatgaggaa tcagtcgacg
    10081 cagcggcaac ttttcgtgct agctgtcagc gcgtgccacg cgtggtcaca tatcggtaca
    10141 tcgatccact tacacaagtg tggctcggga ccgcgcagcg cattggactg cgcgtaattc
    10201 gtacaccgga cgcgtacgcg gcaacggatg gcagcggcac gctcgcgatc ggtgatgaca
    10261 ccgcgctcga tccagacgac agcctcgcgc agatgatctt tcacgagctg tgtcactcgc
    10321 tcgtcgaggg cgaagaatcg ttcacgcgcg cggactgggg catggacaac accggtcctg
    10381 atcacgactg gcgcgagcac gcgtgcctgc gcgtgcaatg ggtgctgaca ggacggcacg
    10441 gcctgcgcac gttgttcgcg ccgacgacgg actttcgcgc gtcgttctgg gatcgcctgt
    10501 ccgacgatgt gttgtcagat cgcaccgatc catctgttca ggccgcgatc ctcggcttgc
    10561 gtcgcgcggc actgccgccg tgggcaccgc acctcgccga agcactggcc gcgaccgcgc
    10621 gcatcgcaca agaggccgcg aagctcgcga ccgagccgac cgtgctgtgg cacggcgtga
    10681 cgccggcgcc ggccgcgcat ccgaccggat tgccagcggg cgccgcgagc ggcacgtgcg
    10741 gcggctgcgc gtggcggttc gagcagcgcg gcgtcgcgaa gtgccgccac acgaacacga
    10801 a
//
LOCUS       DASUFX010000454         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1602611, whole genome shotgun sequence.
ACCESSION   DASUFX010000454 DASUFX010000000
VERSION     DASUFX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602611"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFV99_18305"
     CDS             <1..271
                     /locus_tag="VFV99_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061329.1"
                     /translation="MRHFDAGWTIWLVMAAFALPAFVGASRSETDRVAAILGTACFGY
                     LTYKFRTGVFDLVIHGSLGGIMMGIAVIGGLTTSLMVLAASSRER"
     gene            371..2227
                     /locus_tag="VFV99_18310"
     CDS             371..2227
                     /locus_tag="VFV99_18310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5061330.1"
                     /translation="MDPRARADLLAALAALASDDESAGDALRAIVESMPARLSVIDPS
                     AVFLWTNHPHSKVLGHSMFEYMPEPDHAQARAAIERVLQSGRADRFSGHGPGNRGPHS
                     RYESWIAPIGSPIIALALISRDVSEEWELYDELRDREQRLSLVLRAAGMGMWRFDLRT
                     HTVEFDEAARAIYGFTVSRMDSLTFTTNHIHPDDRAEADRKALESARTRIPYSAQNRI
                     IRTDGQQRWVEVTGSPILDEAGQLVAIMGTITDITERREVEARARQAQRLDAVGSLTA
                     GIAHNFNNMLAAIMPTLSMIEGDVSPISAPLVRGASLATERAAQLVRQLMTFAGRNGN
                     RLRTVEAPGPLVERTVALCRSTFDRVIELRVTIEPELPAICVDVGQIEQALLNLLLNA
                     RDAVADATPPIIQVRVTHASEREIQIVVEDNGPGVPEELRERIFDPFFTTKHVGAGTG
                     LGLATAYAIAREHGGAISCGGESGRGAQFTMTIPGVDLAPTVADPVVRGRGRAQGTVL
                     VVDDDAIVRETVARVLRDAGFTIHTADSADQAIIVLGRQPGVDAVLLDLNMPGSSWRT
                     VVRTLRQDHPKARVVAFTGGVTARDNSVDGWLIKPAHPDAIIDAINQVIVRA"
     gene            complement(2236..>3077)
                     /locus_tag="VFV99_18315"
     CDS             complement(2236..>3077)
                     /locus_tag="VFV99_18315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061331.1"
                     /translation="SSAVDSSMQREKVQLEGEKQTLLVTLYGKALDSHKAHPILGDTY
                     AADVVDQLDVDFKKLRIPKGADVSLPVRAKHLDGWTREFLTAHPAATVLHLGCGLDSR
                     VWRVDPPATVRWYDVDYPDVIELRKRLYPQRHDYTMIGASVTDLRWLDVIPRDNPVLV
                     VAEGLVQYLKPADGTALFRYITETFLSGELIFDAYSKLVVRLMRLVPAARAANVRLSW
                     AIDDPRALVREIPELELVAAVPFLTLPDLTERLPRSNRLATLVLRRFGFVRRAIQHLR
                     YRF"
BASE COUNT          521 a         1041 c          995 g          520 t
ORIGIN      
        1 catgcgccac ttcgatgccg gctggacgat ctggctcgtg atggcggcgt tcgcgctgcc
       61 ggcgttcgtc ggtgcaagtc ggagcgagac cgatcgggtc gccgcgattc tcggcaccgc
      121 gtgcttcggt tacctgacct acaagttccg cacgggcgtg tttgacctcg tcatccacgg
      181 ctcgctcggc ggcatcatga tgggcatcgc ggtgatcggc ggacttacga cgtcgctgat
      241 ggtgctcgcc gcgtcctcgc gcgagcgcta acgaatttct tgcacgggcg atttgcccgg
      301 gcatttccga ggcacgcgcc gcgtatactg gtgccgtata ctggtgccga tgcattgggg
      361 gcatcggtag atggatccac gcgcgcgcgc ggatctgctg gccgcacttg cggcgttagc
      421 gtcggacgac gagtccgccg gcgacgcgtt gcgcgcgatc gtcgagagca tgccggccag
      481 gctgtcggtg atcgatccgt ccgccgtgtt tctctggacg aatcacccgc actcgaaggt
      541 gctcggacat tcgatgttcg aatacatgcc cgagccggat cacgcccagg cgcgtgccgc
      601 gatcgagcgt gtactgcaaa gcggtcgcgc ggaccgcttc tcgggacacg gtccgggaaa
      661 ccgcgggcca cactcgcgct acgaaagctg gattgcaccg atcggctcgc cgatcatcgc
      721 gctcgcgctg atctcgcgtg atgtctccga agaatgggag ctctacgacg agctccgcga
      781 tcgcgaacaa cggctctcgc tcgtgctgcg cgcagccggc atggggatgt ggcgattcga
      841 tctgcgcact cacacggtcg agttcgacga agctgcgcgt gcgatctacg gtttcacggt
      901 gagccgcatg gactcgctca cgttcacgac caatcacatc catcccgatg accgtgccga
      961 ggccgaccgc aaagcgctgg agagcgcgcg cacccggatc ccctactccg cacagaaccg
     1021 gatcattcgc accgacggcc agcagcgctg ggtcgaagta accggcagcc cgattctcga
     1081 cgaggccggt cagctcgtcg cgatcatggg caccatcacg gacatcaccg agcggcgcga
     1141 agtcgaagcc cgcgcgcgtc aggctcaacg actcgacgcg gtcggctcgc tcaccgcggg
     1201 catcgcgcac aacttcaaca acatgctcgc ggcgatcatg ccgacgctgt cgatgatcga
     1261 aggcgacgtg agcccgatct ctgcaccact cgtccgcggc gcatcacttg cgaccgaacg
     1321 cgccgcccag ctcgtgcgtc agctcatgac gttcgccggg cgcaacggca atcgcctgcg
     1381 caccgtcgaa gcacctggcc cactcgtcga acgcacggtc gcactctgtc gctcgacgtt
     1441 cgatcgcgtg atcgagctgc gcgtcaccat cgagcccgag ctacccgcga tctgtgtcga
     1501 cgtcggccag atcgagcagg cgctgctcaa cctcctcctc aacgcgcgcg atgccgtagc
     1561 ggacgccacg ccaccgatca ttcaggtccg cgtcacgcac gcgagcgagc gcgagatcca
     1621 gatcgtcgtc gaggacaacg gtcctggcgt tcccgaagag ctgcgcgaac gcatcttcga
     1681 tccattcttc acgaccaagc acgtcggcgc gggtaccggc ctcggtcttg ccaccgccta
     1741 cgcgatcgcg cgcgagcatg gtggtgcgat ctcgtgcggc ggcgaatccg gccgcggcgc
     1801 ccagttcacc atgacgattc cgggcgtcga cctcgcgccc acggtcgctg atcccgtcgt
     1861 tcgtggccgg ggacgagcgc aggggaccgt gctcgtcgtc gatgacgatg cgatcgtgcg
     1921 cgaaaccgtc gcgcgtgtgc tcagggatgc cggtttcacg atccacacgg ccgacagcgc
     1981 cgaccaggcg atcatcgtgc tcgggcgcca gcccggcgtc gatgccgtgc tgctcgatct
     2041 caacatgccc ggctcgtctt ggcgcacggt cgttcgcacg cttcgccaag atcatccgaa
     2101 ggcgcgtgtc gtcgcgttca ccggcggcgt caccgcgcgc gacaactccg tcgacggctg
     2161 gctgatcaag cccgcacatc cggacgcgat catcgacgcg atcaaccaag tcatcgtgcg
     2221 cgcgtgacca tcggctcaga accgatagcg caggtgttga atcgcgcgcc gcacgaaccc
     2281 gaaccgacgc agcaccagcg tcgccaaacg gttgctgcgg ggtagtcgct cggtcagatc
     2341 cggcagcgtg aggaacggca ccgctgcgac gagctcgagc tcgggaatct cgcgcacgag
     2401 cgcacgcgga tcgtcgatcg cccaggagag ccgcacgttc gcggcgcgcg ccgccggcac
     2461 gagccgcatc agcctgacga ccagcttgct gtacgcgtcg aagatgagct cgccgctcag
     2521 aaacgtttcc gtgatgtagc ggaacagcgc ggtgccatcc gcgggcttca ggtactgcac
     2581 gaggccttcg gcgacgacga gcaccgggtt gtcgcgcgga atcacgtcga gccagcgcag
     2641 gtcggtgacc gacgcgccga tcatcgtgta gtcgtgacgc tggggataca ggcgcttgcg
     2701 cagctcgatg acgtcgggat agtcgacgtc gtaccagcgc acggtggcgg gcggatcgac
     2761 gcgccacacg cgactgtcca ggccacagcc gaggtgcagc accgtcgctg ccggatgcgc
     2821 ggtgaggaac tcgcgcgtcc agccatcgag gtgtttcgcg cgtaccggca acgagacatc
     2881 cgcgcccttg ggaatccgta gcttcttgaa atcgacgtcg agctggtcga cgacatcggc
     2941 cgcataggtg tcgccgagga tcggatgcgc cttgtggctg tcgagcgctt tgccgtagag
     3001 cgtgacgagg agcgtctgct tctcgccctc gagctgaacc ttctcgcgct gcatgctcga
     3061 gtctaccgcc gacgagg
//
LOCUS       DASUFX010000455         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_907158,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000455 DASUFX010000000
VERSION     DASUFX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907158"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..564)
                     /locus_tag="VFV99_18320"
     CDS             complement(112..564)
                     /locus_tag="VFV99_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061332.1"
                     /translation="MKKLILLVILGLAGSAAAQPGSAAQPVAQPAAQPAPPPAEQPAP
                     PAVPPVPKASAEELRKTCADAMNADPAFAKSIVETVDKQIDQKTIDAHNAAAAQIAEN
                     ERHVIYAYAAMWILAAAFVVFLWWRQQALKAELAQLRRDLDAAAKESK"
     gene            complement(561..1274)
                     /gene="ccsA"
                     /locus_tag="VFV99_18325"
     CDS             complement(561..1274)
                     /gene="ccsA"
                     /locus_tag="VFV99_18325"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5061333.1"
                     /translation="MKRFTVPVLAALSALTLAVAIHYVFIKAPLQYSFTAEGMLNGSS
                     LFFNQKIFYFHVTQAFMLFVAVGLAGVASAIFLKTRNPKWDDIAAASVDVAVMFGAVV
                     LATGSIWAKAAWDTWWNWEPRLTMSLLLWLILVGYWLVRKFAGPSADRVAAGMAIFGM
                     VGVPFIYKMVGEDSHPPSGMEGVVMNLGTEYKLAFLFSSIGFLLWFLTLVIVRVQGTR
                     AEREARELRERGLDLGVLQ"
     gene            complement(1271..1957)
                     /locus_tag="VFV99_18330"
     CDS             complement(1271..1957)
                     /locus_tag="VFV99_18330"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5061334.1"
                     /translation="MSSLLRQAGRVAWKDLRVELRSKEIVMTMIFFATLLAAIYALAF
                     KGQDPRAVRGAVPALLWVSLAFSGTIALGRAFDRERENDTMRALLLAPVPRLAVFLGK
                     AIAMTVLILSVALIVVPLLALWLNAPLFKHTGPLALIVLLGSVGIALVGSVFSATLLK
                     VRSRDVLLPVILYPLLIPLFIAGTRTTAVLVAEIPNLEQAWFWIGFVGIYDAVFLVLS
                     LWTFESLVIE"
     gene            complement(1959..>2542)
                     /locus_tag="VFV99_18335"
     CDS             complement(1959..>2542)
                     /locus_tag="VFV99_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5061335.1"
                     /translation="KTTLLGVVSTLVRPSSGGVTYHSGGKALEAEDVRRQIGLLAHQS
                     LCYGELSAIENLEFFAELYDVDASAKAIKAVLDQVGLDERARTRAVRTYSRGMQQRVA
                     LARALLTKPSLLLLDEPFTGLDRDGAINLGKQLGDLRSSGAIVVVVTHDLEAIAGRCD
                     HVAILRRGGLVFEERADTYSYEQLKHIYHQHAN"
BASE COUNT          503 a          809 c          821 g          409 t
ORIGIN      
        1 cgcgcttgcg ttgcagatcg aactgattgc gagccgcgcg ggtaccgagg atgaagccga
       61 tgaacatacc gaccatcagc gcgcccggaa tgaacaggat gtgactcgaa gtcacttgct
      121 ctccttcgcg gccgcatcga gatctctgcg tagctgcgcg agctcggcct tcagtgcttg
      181 ctgccgccac cacaagaata cgacgaacgc ggcggcgagg atccacatcg cggcatacgc
      241 gtagatgacg tggcgctcgt tctcggcgat ctgtgccgcc gcggcgttat gcgcatcgat
      301 ggtcttctga tcgatctgct tgtcgacggt ctcgacgatc gacttcgcga acgcggggtc
      361 tgcgttcatc gcgtccgcac aggtcttgcg cagctcttcg gccgacgcct tcggaaccgg
      421 aggcacggca ggcggcgcgg gttgttctgc cggcggcggt gcgggttgcg cggcgggttg
      481 cgctactggt tgcgcggccg aacctggttg cgccgcggca ctacctgcga gcccgaggat
      541 cacgaggagg atgagcttct tcattgcaga actcccagat cgagaccgcg ctcgcgcagc
      601 tcgcgtgctt cgcgttcggc gcgcgtgcct tgcacgcgaa cgatcacgag cgtcaagaac
      661 cacagcaaga acccgatcga cgagaacagg aatgccaact tgtactcggt gccgaggttc
      721 atgacgacac cctccatgcc cgacggcggg tgcgagtctt cgccgaccat cttgtagatg
      781 aacggcacgc cgaccatgcc gaagatcgcc atgccggccg cgacacgatc cgcgctcggg
      841 cccgcgaact tccgcacgag ccagtagccg acgaggatca gccagagcag cagcgacatc
      901 gtcagccgcg gctcccagtt ccaccacgtg tcccacgcgg ctttcgccca gatcgagccg
      961 gtcgcgagca cgactgcgcc gaacatcacg gcgacatcga cggaggccgc tgcaatgtcg
     1021 tcccatttcg gattgcgcgt cttcaagaag atcgccgacg cgacgccggc cagcccgacc
     1081 gccacgaaga gcatgaacgc ctgggtgacg tggaagtaga agatcttctg gttgaagaac
     1141 aggctcgaac cgttgagcat gccctcggcg gtaaagctgt actgcagcgg cgccttgatg
     1201 aagacgtaat ggatcgcgac ggccagcgtg agagctgaca gcgcggcgag gacgggaacg
     1261 gtgaacctct tcattcgatc accagggact cgaaggtcca cagggacagt accaggaaga
     1321 cagcgtcata gattccgacg aacccgatcc agaaccaggc ctgctccagg ttcgggattt
     1381 cggcgaccag caccgcggtg gttctcgtac ctgcgataaa caacgggatc agcagcggat
     1441 agagaatgac agggagtagc acgtctcgcg agcggacctt gagcagcgtc gcgctgaaca
     1501 ccgagccgac gagtgcgatg ccgacggagc ctagcaacac gatcagtgcg agcgggcctg
     1561 tgtgcttgaa cagcggcgcg ttgagccaca gcgcgagcag cggcaccacg atgagcgcga
     1621 ccgaaaggat cagcacggtc atcgcgattg ccttgccgag gaacaccgcg agccgcggta
     1681 caggagccag caacagagcg cgcatcgtgt cgttctcgcg ctcgcgatcg aacgcgcggc
     1741 cgagcgcgat cgtgccggaa aacgccagcg agacccacag cagcgccggt accgcaccgc
     1801 gcacggcgcg gggatcctgc cctttgaacg ctaacgcgta gatcgcggcg agcaacgtcg
     1861 cgaagaagat catcgtcatc acgatctcct tgctgcgcag ctcgacgcgg agatctttcc
     1921 acgcgacgcg accggcttgg cgaaggagcg acgacatctc aattggcgtg ctgatgatag
     1981 atgtgtttga gctgctcgta ggagtacgtg tccgcacgtt cttcgaacac gagcccaccg
     2041 cggcgcaaga tcgcgacgtg atcgcagcgc ccggcgatcg cttcgagatc gtgcgtgacg
     2101 acgacgacga tggcaccgct cgacctgaga tcgccgagct gcttgccgag attgatcgca
     2161 ccgtcgcgat cgaggccggt gaacggctcg tcgagtagca acagcgacgg cttcgtgagt
     2221 agcgcgcggg cgagcgcgac ccgctgctgc atgccgcgcg agtaagtgcg gaccgcgcgc
     2281 gtgcgcgcac gctcgtcgag cccgacctga tcgagcacgg ccttgatcgc ctttgcactg
     2341 gcgtcgacgt catagagctc ggcgaagaac tcgaggttct cgatcgcgga cagctcgccg
     2401 tagcagagcg actggtgcgc gagcaggccg atctggcggc gcacgtcctc cgcttcgagc
     2461 gccttgccgc cgctgtgata cgtgacgcca cccgagctcg gccgcaccaa tgtagagacc
     2521 acgccgagca gcgtcgtctt cc
//
LOCUS       DASUFX010000456         8047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1323533, whole genome shotgun sequence.
ACCESSION   DASUFX010000456 DASUFX010000000
VERSION     DASUFX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323533"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VFV99_18340"
     CDS             <1..1318
                     /locus_tag="VFV99_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5061336.1"
                     /translation="VAAVTDYPFFFTWTAQNQAKPLELTGGEGAWFTTKDGSRWLDLG
                     ALAYQVNVGHGNKRIVEAIKRQADELCLSTPNAVYPAKVELAKRLLAMAGPGFSKVFF
                     TLGGAEANENALKIARLVTKRLKNIARYSSYHGASMGALSLTGDWRRPLVEPGIPGVI
                     HVEDCYCDRCPFDKTLEACGRECATNIGDVMRREGAGAGAAAPGSLAGAVAAVFLEPV
                     PGANGVLVPPPEYWPLVRKACDENGALLVADEVLTGFGRTGKPFGFQHWDVVPDMITV
                     AKGLASGYATIGAVIVHERIAKYFDDHVLACGLTYYAHPTACAAALETMKVYDDDKLY
                     ENAERLGPVLRRELDAVAARVAPSVFVRSLGLLGVIEFGKVTDASAASWKKLGAELSA
                     RKLSLHVEPSRGTAIFSPPLCISEQELVSGVRAFGHAAVAAFGART"
     gene            1315..2028
                     /locus_tag="VFV99_18345"
     CDS             1315..2028
                     /locus_tag="VFV99_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX5061337.1"
                     /translation="MTGKHGQKVVTSIEEALAPLRDGMTIMVGGFGLSGNPEALIKGV
                     VARGVKELTLVSNNAGNLGKGLATWLKAGIIRKVICSYIGSNEDLHTRMASGEVKVEI
                     TPQGTLAERMRAAGAGIPAFFTPTGVGTVVEDGKEVREIDGRRYILERALHADVALVR
                     AQLSDHFGNLRFWRTAKNFNPVMATAAKLTIVECDRLVDNGTIDPDAVHLPGIFVHKI
                     IHVPEHENAFEYRTVRKRP"
     gene            2025..2648
                     /locus_tag="VFV99_18350"
     CDS             2025..2648
                     /locus_tag="VFV99_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX5061338.1"
                     /translation="MTYSTEDMARRAAREIPAGSIVNLGIGLPTQVADYLPEGHAWLH
                     SENGLLGMGPFPYEGDEDPNLINAGKQTVTVLPAGSTFDSATSFAMIRGGHVDLAILG
                     AMQVSAGGDLANWAVPGGKVMGIGGAMDLASGCRRVIAMMQHTTKKKEFKLVQKCEYP
                     LTATRVVSLVITELGAFEPTGHAFRVVDLAPGVTREQAEQATGAPLI"
     gene            2797..3027
                     /locus_tag="VFV99_18355"
     CDS             2797..3027
                     /locus_tag="VFV99_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061339.1"
                     /translation="MNTKNEELTTIDSADLDTVTGGMWGPIIAQGLSWLGSMFGGGTN
                     VGVQNGNNNRQAQGNTGPTTLGDGSPIKGPQQ"
     gene            3070..3321
                     /locus_tag="VFV99_18360"
     CDS             3070..3321
                     /locus_tag="VFV99_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061340.1"
                     /translation="MDIKHTDTNELASIDTADLEHVTGGGWAGDAWNWVKTNVGGLFG
                     GGGGGGGINVGVQNGNNNRQAQGNSGSTTIGDNSPIGGQ"
     gene            3323..3553
                     /locus_tag="VFV99_18365"
     CDS             3323..3553
                     /locus_tag="VFV99_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061341.1"
                     /translation="METYELATISPEELAFVEGAWLNVGVAWGDNNRVSVGTGTRGLN
                     FQFAWGNNNHVETGQGPTFRLNWGSNNTFGSR"
     gene            3563..3820
                     /locus_tag="VFV99_18370"
     CDS             3563..3820
                     /locus_tag="VFV99_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061342.1"
                     /translation="MSDKKDKGVIRLHEGEHVKFAIVSGDDLEVVVGGGWAGDAWNWV
                     KGSVGNFFGGLNIGIANGNNNRVAVGNQGPTTLGDNSPIGK"
     gene            3826..4026
                     /locus_tag="VFV99_18375"
     CDS             3826..4026
                     /locus_tag="VFV99_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061343.1"
                     /translation="MNDQQETVSPAELDAVTGGVGWRCTGFRVPTGFLGGLNISITSG
                     NNKVVAQGNLGATTLADNQPVK"
     gene            4183..4398
                     /locus_tag="VFV99_18380"
     CDS             4183..4398
                     /locus_tag="VFV99_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061344.1"
                     /translation="MSDQFETVESAELDGVTGGILPWLIAGAAAAGAAWYFGGGINVG
                     VANGDNNRVAVGNQGPTTLGNNSPVQQ"
     gene            4407..4724
                     /locus_tag="VFV99_18385"
     CDS             4407..4724
                     /locus_tag="VFV99_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061345.1"
                     /translation="MNEQDFETVEPAELDSVTGGILPQLIAGAAAAGAAWYSGGGINV
                     GIANGNNNRVAVSGGGIGIANGNNNRVMVNGGIGIANGNNNRIEMGNPSPTTIDNNSP
                     INQ"
     gene            complement(4752..5564)
                     /gene="argB"
                     /locus_tag="VFV99_18390"
     CDS             complement(4752..5564)
                     /gene="argB"
                     /locus_tag="VFV99_18390"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5061346.1"
                     /translation="MSLVVKIGGDVIGSGEAKALAADLRVLVDEGALVAIVHGGGPQA
                     TALQKQLGIETKQVAGRRVTDAATLDVMKMVLAGKLNVDLCAALNAAGVSAVGLHGAS
                     GLVVRAVRRPPKKYPGAGDEPVDMGFVGDVVGFNLELLEALWATKYVPVIACLGADAE
                     GGVYNINADMVGNQLAAALKADRLFLVTSTPGVLRDVNDPSSRLAQLTCAEARDAIRS
                     GVVTGGMIPKLEEAMAVVDQGVGAIHILGKLGPGDLVRAVREPGSVGTTLRP"
     gene            complement(5561..6616)
                     /gene="argC"
                     /locus_tag="VFV99_18395"
     CDS             complement(5561..6616)
                     /gene="argC"
                     /locus_tag="VFV99_18395"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5061347.1"
                     /translation="MAKRFKAAIIGGSGYGGAEMARRLLMHPDVELVRVASIDHVGEP
                     LGAVHPNLDGATELVFEDLPPAEAAKGCDVALLALPHKVTAAKVPELIAAGVKIVDMS
                     GDFRLRDAAVYEKFYGAKHPHPELLGTFVYGLPELNRAAIKAAKYVASPGCFATTIEL
                     ALLPLARAGMLDSAVVHVMGITGSSGSGIAPQAGTHHPSRAGNLKTYKPLEHQHVPEI
                     VQTLADGGAKGIELRFVPVSAPLTRGIFASCFLELDAKVDKNQLASLFDESYAREAFV
                     RRPKARLPEVVAVAGSNYAEVGFAVGPVANGKRTVTVFSAIDNLIKGGAGQAIQNMNL
                     VLGVDERASLEDVGNWP"
     gene            6716..7816
                     /locus_tag="VFV99_18400"
     CDS             6716..7816
                     /locus_tag="VFV99_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5061348.1"
                     /translation="MLALARSWVEINSYTSNVEGVNRVGALLREAFSLPALTGSVIPG
                     EAGYGDHLVWRTPASGAPILLIGHHDTVFPPGHFEGWKEDGPRAVGPGALDMKGGLAI
                     IRTALAALADVGVLANLPLVVISVADEEVGSPCSAPHLRELAKGAACALVFESGRAHD
                     MIITQRKGVGAMTVIAHGKAAHSGNNHKDGANAIWALAKFVDAAQQLTDYGKGVTVNV
                     GQFSGGTSKNTVPERAECKLDLRYQTVADADALVAGLRAAAAAAQIPGVTLEVQGGVN
                     RLPLERSPASAALRDEYAACARASGLGDGEAPLLGGGSDANTVAPLGVPAIDGLGPRG
                     AGFHTTTEYVELATFVPKAEALVRFLYARLPA"
BASE COUNT         1481 a         2693 c         2633 g         1240 t
ORIGIN      
        1 agtggcagct gtgaccgatt atccgttctt cttcacctgg accgcgcaga accaagccaa
       61 gccgctcgag ctgacgggcg gcgagggcgc ctggttcacc acgaaagacg gctcgcgctg
      121 gctcgacctc ggcgcgctcg cctatcaggt caacgtcggg cacggcaaca agcgcattgt
      181 cgaggcgatc aagcgccagg ccgacgagct gtgcctgtcg acgccgaacg ccgtgtatcc
      241 cgcgaaggtc gagctcgcga agcggctgct cgcgatggcg ggcccgggct tctccaaagt
      301 gttcttcacg ctcggcggcg ccgaggcgaa cgagaacgcg ctcaagatcg cacgcctcgt
      361 caccaagcgc ctcaagaaca tcgcgcgcta ctcgagctat cacggcgcct cgatgggcgc
      421 gctgtcgctc accggtgact ggcgccgccc gctcgtcgag cccggcattc cgggcgtgat
      481 ccacgtcgag gactgctact gcgatcgctg tccgttcgac aaaacgctcg aagcttgtgg
      541 acgcgagtgc gcgacgaaca tcggcgacgt gatgcggcgc gaaggcgcgg gtgcgggcgc
      601 tgccgctcct ggctcacttg cgggcgcggt cgccgccgtg ttcctcgagc cggtgccggg
      661 tgccaacggc gtgctcgtgc cgccgcccga gtactggccg ctcgtacgca aggcgtgcga
      721 cgagaatggc gcgctgcttg tcgccgacga ggtgctcacg ggcttcggtc gcaccggcaa
      781 gccgttcggg ttccagcact gggacgtcgt gcccgacatg atcacggtcg cgaagggcct
      841 cgcgtcgggg tacgcgacga tcggtgcggt gatcgtgcac gagcgcatcg cgaagtactt
      901 cgacgaccac gtgctcgcgt gcggcctcac gtactacgcg cacccgaccg catgcgcggc
      961 ggcgctcgag acgatgaagg tctacgacga cgacaagctg tacgagaacg ccgagcgcct
     1021 cggcccggtg ctgcgccgcg agctcgatgc ggtggccgca cgcgtcgcgc ctagcgtgtt
     1081 cgtccgctcg ctcggcctcc tgggcgtaat cgaattcggc aaggtcaccg acgctagcgc
     1141 tgcaagctgg aagaagctcg gtgcggaact gtcagcgcgc aaactgtcgt tgcatgtcga
     1201 gccgagccgc ggcacggcga tcttctcgcc gccactctgt atcagcgagc aagagctagt
     1261 cagcggcgtg cgtgctttcg ggcatgcggc cgtggcagca ttcggcgcgc gtacatgact
     1321 ggcaaacacg gacagaaggt cgtcacctcg atcgaagagg cgctcgcgcc gctccgcgac
     1381 ggcatgacga tcatggtcgg cggcttcggc ctgtcgggaa atcccgaggc gctgatcaag
     1441 ggcgtggtcg ctcgcggcgt caaagagctc acgctcgtct cgaacaacgc aggcaacctc
     1501 ggcaaaggtc tcgcgacgtg gctcaaggcc ggcatcatcc gcaaggtgat ctgcagctac
     1561 atcggtagca acgaagacct ccacacgcgt atggcttcgg gcgaagtgaa agtcgagatc
     1621 acgccgcagg gcacgctcgc cgagcgcatg cgcgccgcgg gtgcgggcat ccccgcgttc
     1681 ttcacaccga ccggcgtcgg caccgtcgtc gaagacggca aagaggtgcg cgagatcgac
     1741 ggacgtcgct acatcctgga gcgcgcgcta cacgctgacg tcgcgctcgt gcgtgcacag
     1801 ctctccgacc acttcggcaa cctgcggttc tggcgcacgg caaagaattt caatccggtg
     1861 atggcgaccg ctgcgaagct gacgatcgtc gaatgcgatc ggctcgtcga caacggcacg
     1921 atcgatcccg acgccgtcca tctaccgggc atcttcgtcc acaagatcat ccacgttccc
     1981 gagcacgaga acgcgttcga ataccgcacg gtgaggaagc ggccatgacg tactccaccg
     2041 aagacatggc gcgtcgcgcg gcgcgcgaga ttcccgccgg ctcgatcgtc aacctcggca
     2101 tcggtctgcc gacgcaggtc gcggactatt tgcccgaagg ccacgcgtgg ctgcactcgg
     2161 agaacggtct gctcggcatg ggcccgttcc cgtacgaagg cgacgaggat ccgaacctca
     2221 tcaacgccgg caagcagacc gtcaccgtct tgcccgcggg ttcgacattc gactcggcga
     2281 cgtcgttcgc gatgattcgc ggcggtcacg tcgatctcgc gatcctcggc gcgatgcagg
     2341 tgtcggcggg cggcgatctc gcgaactggg cggtgccggg cggcaaggtg atgggcatcg
     2401 gcggcgcgat ggatctcgcg tcgggctgcc gtcgcgtgat cgcgatgatg cagcacacga
     2461 cgaagaagaa agagttcaag ctcgtgcaga agtgcgagta cccgctgacc gcaacgcgcg
     2521 tcgtgtcact cgtgatcacc gagctcggtg cgttcgagcc gaccggacac gcgttccgcg
     2581 tggtcgatct tgcgcccggt gtcacgcgcg agcaggctga gcaagcgacc ggcgcgccgc
     2641 tgatctaagc ggtcgcatcc tcacggtttc acttcgtctc gcctgggttg cgccgatccc
     2701 tgcgcaacgc acggcaaatt gccgaaacga gtggcggctg ccgtcggcat ggcggctgct
     2761 ctagccgcca tcgaccctaa acaaggagca aacgggatga acaccaagaa cgaagagctc
     2821 accacgatcg attcggctga cctcgacacc gtcacgggcg gcatgtgggg cccgatcatc
     2881 gcgcagggcc tctcctggct cggcagcatg ttcggcggcg gcaccaacgt gggcgtgcag
     2941 aacggcaaca acaaccggca ggcgcaaggc aacaccggcc cgacgacgct gggcgacggc
     3001 agcccgatca agggcccgca gcagtaatcc gaacaaccgt tctctcaacc agcacgaagg
     3061 agcagcacca tggacatcaa gcacacggat acgaacgagc tagcaagcat cgacaccgcg
     3121 gacctcgagc acgtcaccgg cggtggctgg gcgggcgacg cgtggaactg ggtcaagacg
     3181 aacgtcggcg gcctgttcgg cggcggcggc ggcggcggcg ggatcaacgt cggcgtccag
     3241 aacggcaaca acaaccgcca agcgcagggc aacagcggct cgacgacgat cggcgacaac
     3301 agcccgatcg gaggccagta gcatggagac gtacgagctc gcgacgattt ctcccgagga
     3361 gctcgcgttc gtcgaaggcg catggctgaa cgtcggcgtg gcgtggggcg ataacaaccg
     3421 cgtctccgtc ggcaccggca cacgcggcct caacttccag ttcgcgtggg gcaacaacaa
     3481 ccacgtcgag accgggcagg gtccgacgtt ccgcctcaac tggggcagca acaacacgtt
     3541 cggcagccgc taggagatga ccatgagcga caagaaggac aagggcgtca tccggcttca
     3601 cgaaggcgag cacgtcaagt tcgccatcgt tagcggcgat gatctcgagg tcgtcgtcgg
     3661 cggtggctgg gcaggcgacg cgtggaactg ggtgaagggt tccgtcggca acttctttgg
     3721 cggtcttaac atcggcatcg ccaacggcaa caacaaccgc gtcgcggtcg gcaaccaagg
     3781 tccgacgacg ctcggcgaca acagtccgat cgggaagtga caccgatgaa cgatcaacaa
     3841 gaaaccgtgt cgccggcaga gctcgacgcc gtaaccggcg gcgtcggctg gcgctgcacc
     3901 ggcttccgag tgccaactgg tttcctcggc ggcctgaaca tctcgatcac gagcggcaac
     3961 aacaaggtcg tcgcgcaggg caacctgggc gcgacgacgc tcgctgataa ccagcccgtg
     4021 aagtaacgga cacggcacat gaccgatcaa ccgagtgcca acgggttttc tcggcggcct
     4081 gaacatcgcg atcacgagcg gcaacaacaa gctcgtcgcg cagggcaacc agggcgcgac
     4141 gacgctcgct gacaaccagc ccgtgaagta acggagatgc acatgagcga tcaattcgag
     4201 accgtggaat cggcagagct cgacggcgtg acgggcggca tcttgccgtg gctgatcgcc
     4261 ggcgcagcag cggccggtgc agcgtggtac ttcggcggcg gcatcaacgt cggcgtcgcc
     4321 aacggcgata acaaccgcgt cgcagtgggc aatcaaggcc cgacgacgct cggcaacaac
     4381 agcccggtgc agcagtaatg cgcgtcatga acgagcagga cttcgagacc gtggagccgg
     4441 cagagctcga cagcgtgacg ggcggcatcc tgccgcagct gatcgcgggc gcagcggcgg
     4501 ccggtgcagc ctggtactcc ggcggcggta tcaacgtcgg catcgcgaac ggcaacaaca
     4561 atcgcgtcgc ggtgagcggc ggcggcatcg gcatcgccaa cggcaacaac aaccgcgtca
     4621 tggtgaacgg cggcatcggc atcgccaacg gcaacaacaa ccgcatcgag atgggcaatc
     4681 caagcccgac gacgatcgac aacaacagcc cgatcaatca gtaacggctg gcgcgacgca
     4741 cggctgctcg ttcagggccg cagcgtcgta ccaaccgagc caggctcgcg cacggcgcgc
     4801 accagatcgc cggggccgag cttgcccaag atgtgaatcg cgccgacgcc ttggtcgaca
     4861 acggccatgg cttcttcgag tttcggaatc atgcctccgg tgacaacacc ggacctgatg
     4921 gcgtcgcgcg cttctgcgca agtcagctgg gcgagacgtg acgagggatc gttgacgtcg
     4981 cgcagtacgc ccggcgtcga cgtgaccaag aacaaccgat ctgctttgag cgcggcggca
     5041 agctggttgc cgaccatgtc ggcgttgatg ttgtagacgc cgccttcagc atcggcaccg
     5101 aggcaggcga tgacgggaac gtacttcgtg gcccacagcg cttcgagcag ctcgaggttg
     5161 aagccgacga cgtcgccgac gaatcccata tcgacaggct cgtccccggc gccgggatac
     5221 ttcttcggcg gccgtctcac ggcacgcacg acgagacccg acgcaccgtg cagaccgacc
     5281 gccgacacgc cggcggcatt cagcgccgcg cacagatcga cgttgagctt gccggcgagc
     5341 accatcttca tgacgtcgag cgtcgcagcg tcggtcaccc gacgacctgc gacttgcttc
     5401 gtctcgatgc cgagctgctt ttgcagcgcg gtcgcctgcg ggccgccgcc atgaacgatc
     5461 gcgaccagcg cgccttcatc gacgagcacg cgcaaatcag cggcgagcgc ctttgcttcg
     5521 cccgagccga tgacgtcgcc gccgatcttg acgacgagac tcacggccag ttcccgacgt
     5581 cttcgaggct ggcgcgctcg tcgacgccga gcacgaggtt catgttctgg attgcctggc
     5641 cggcgccgcc cttgatcagg ttgtcgattg ccgagaacac cgtgaccgtg cgcttgccgt
     5701 tcgccaccgg gccgaccgcg aatccgactt cggcgtagtt gctaccggca actgcgacga
     5761 cttccggcaa ccgcgccttc ggccggcgta caaacgcctc gcgcgcgtag ctctcgtcga
     5821 acagcgaagc gagctggttc ttgtcgacct tggcgtcgag ctcgaggaag cacgacgcga
     5881 agatgccgcg cgtgagcggt gccgacaccg ggacgaagcg cagctcgatg cccttcgcac
     5941 cgccatcggc gagcgtctgc acgatctcgg gcacgtgctg gtgctcgagc ggcttgtacg
     6001 tcttgaggtt gcccgcacgg ctcgggtgat gcgtgccggc ctgcggggcg atgccggatc
     6061 ccgaggagcc ggtgatgccc atgacatgga ccacggcgct atccagcatg cccgcgcgcg
     6121 cgagcggcaa cagcgcgagc tcgatcgtcg tcgcgaagca gcccggcgat gcgacgtact
     6181 tcgccgcctt gatcgcggcc cgattcaatt ccggcaagcc atacacgaac gtgcccagca
     6241 gctccggatg cggatgcttc gcgccgtaga acttctcgta gacggcagca tcgcggagcc
     6301 ggaagtcgcc cgacatgtcg acgatcttca cgccggcggc gatcagctcg ggcaccttcg
     6361 cggcggtcac cttgtgcggt agcgcgagca acgcgacgtc gcagcccttc gcggcttcgg
     6421 caggcggcaa atcctcgaac acgagctcgg tcgcgccgtc gaggttcggg tgcaccgcgc
     6481 cgagtggctc gccgacgtga tcgatcgatg ccacgcgcac gagctcgacg tccggatgca
     6541 tgagtagccg gcgcgccatc tctgcgccgc cgtatccgct accgccgatg attgctgcct
     6601 tgaatcgctt tgccatcgtg ggccgaggta tgccatgcct cgcaccaccg tgtcacacac
     6661 aggcgctaca cccctcgata acgcgctcgc tgccgtgcgc agccgcgaag ctgagatgct
     6721 cgcgctcgct cgcagctggg tcgagatcaa cagctacacg tcgaacgtgg aaggcgtaaa
     6781 tcgtgttggc gcactgctcc gcgaagcgtt ttcactccct gctttgaccg gcagcgtcat
     6841 tccgggtgaa gccggctacg gtgatcacct cgtctggcgc acccccgcat cgggagcgcc
     6901 gatcttgctc atcggtcatc acgacaccgt gtttccgccg ggccacttcg aaggctggaa
     6961 agaagacggt cctcgcgcag tcgggccggg cgcgctcgac atgaagggcg gcctcgcgat
     7021 cattcgcacc gcgctcgcgg cactcgcgga cgtcggggtg ctcgcgaacc ttccgctcgt
     7081 cgtcatctcg gtcgccgacg aagaggtcgg ctcgccgtgc tctgcgccgc acctgcgcga
     7141 gctcgcgaag ggcgcagcgt gcgcactcgt gttcgagtcc ggccgcgccc acgacatgat
     7201 catcacgcaa cgcaaaggcg tcggcgcgat gaccgtgatc gcccacggca aagccgcgca
     7261 cagcggcaat aaccacaagg acggcgcgaa cgcgatctgg gcgctcgcga agtttgtcga
     7321 tgccgcccaa cagctcaccg actacggcaa gggtgtcacc gtcaacgtgg gccagttctc
     7381 cggcgggacg tcgaagaaca ccgtgccaga gcgcgccgag tgcaagctcg accttcgtta
     7441 ccagaccgtc gccgatgccg acgcactcgt cgctggcctg cgcgccgccg cagcggcggc
     7501 ccagatcccg ggtgtcacgc tagaagtgca gggcggcgtc aatcgtctcc cgctcgagcg
     7561 cagccccgca tccgcagcgc tgcgcgacga atacgccgcg tgtgcgcgcg catcgggtct
     7621 cggcgatggt gaagcgccgc tgctcggcgg cggctccgac gcgaatacgg tcgcgccgct
     7681 cggtgtcccc gcgatcgatg gcctcggccc acgtggcgcc ggctttcaca cgacgaccga
     7741 gtacgtcgag ctcgcgacgt tcgtgcccaa ggccgaagcc ctcgtgcgct ttctctacgc
     7801 tcgcttgcct gcgtaaccgc agccaattgt tgcaccgaac cgtgcgcagg cgctcggtcg
     7861 ccgcgtaggg catcggcgca ccttgcgtcg ccagcgaacg caacgcgaaa ctgggaccgc
     7921 cgataccgcc gccatgcccg agatcgtgcg tacgcttgtt tgtctcgcga tcgtgtcgac
     7981 ctcgagcctc gccgcggcgc agatcgtcga tccgtggcca gacaaggatg ccgcgcctgc
     8041 tacagac
//
LOCUS       DASUFX010000457         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1184702, whole genome shotgun sequence.
ACCESSION   DASUFX010000457 DASUFX010000000
VERSION     DASUFX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184702"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFV99_18405"
     CDS             complement(<1..1076)
                     /locus_tag="VFV99_18405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5061349.1"
                     /translation="MKYLPLIWAGLWRKRASTVLTLFCVVFAFALFGLLHGFSAALDG
                     IIDAMSDTRLRTMSRVNITQPLPLAHRARMQTVPGVDEVSYYNFFAGYYQDRRNSIQV
                     GAIDVASFNAIYPDIKIEPQYIDAMLHTRNGALVGEDLAKQQGWKIGDRLPLGSAVWQ
                     RKDGAENWDFEIVGSYRSPSGRVPTSELWINYDYFDEARAAANGTVTLYFLKIHDSSS
                     AAAISERIDALFANSTNETQTQSEKDWVRAQIAQIGDIATFVNLIIAAVMFALLFVTF
                     NTMMQSVRARTPELAVLKTYGFSNTAVMSLVLAESVILCTIAAALGIGIAAVISPPIY
                     RQVGAGGLNLPLPVVVAGIGLAIV"
     gene            complement(1073..1768)
                     /locus_tag="VFV99_18410"
     CDS             complement(1073..1768)
                     /locus_tag="VFV99_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5061350.1"
                     /translation="MTALVSIKQAVKGYTRGKQRVEVLHSIDLTVERGEFLALMGPSG
                     SGKTTLLNLIGGLDRPDRGEIVVAGERLDQLSSGQLAKWRARNVGFIFQFYNLLPVLT
                     AERNVEVPLLLTKLSSAERKRNVAAALELVGLADRAKHKPGELSGGQQQRVAIARALV
                     ADPTLLVCDEPTGDLDRETSESILKLLELLNEHQHKTIVMVTHDPRAANFASRRLYVD
                     KGSLTNAPLGVAA"
     gene            complement(1802..>2484)
                     /locus_tag="VFV99_18415"
     CDS             complement(1802..>2484)
                     /locus_tag="VFV99_18415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5061351.1"
                     /translation="RAVEVAKARVELAQRNLDDTVVRAPFAGIVTVKAAQEGEMVSPM
                     SAGGGFTRTGIGTIVDMSSLEVEVDVSENFIQRVSTDQPATITLNAYPDWSIPAHVIA
                     IIPTADRAKATVKVRVGFAVGDPRILPEMGARVAFHDAKAPRDAAAASAVAQQPVLVP
                     KESVQADGDTGVVFVIADGGVERRTVRLGPQSAAGQVVLAGLKAGTRLASGDLSSLTD
                     GSRVRVEE"
BASE COUNT          428 a          836 c          794 g          426 t
ORIGIN      
        1 aacacgatcg cgagaccaat acccgctacg acaacgggca gcggcaaatt caacccgccg
       61 gccccaacct gacggtagat cggcggcgag atcacggcgg cgattccgat cccgagcgcc
      121 gcggcaatgg tgcagaggat caccgactcg gccaacacga ggctcatcac agccgtgttg
      181 ctgaagccgt aggtcttcaa caccgcgagc tcgggagtcc gcgcacgaac cgactgcatc
      241 atcgtgttga acgtcacgaa cagcagcgcg aacatgaccg ccgcgatgat gaggttgacg
      301 aaggtggcaa tgtcgccgat ctgggcgatc tgagcccgta cccagtcctt ctcgctctgc
      361 gtctgagtct cgttcgtcga gttcgcgaac aacgcatcga tccgctccga aatcgcggcc
      421 gcgctgctcg aatcgtggat cttgagaaaa tagagcgtca ccgtgccatt cgcggcggcg
      481 cgcgcttcgt cgaaatagtc gtagttgatc cacagctcgc tcgtcgggac cctacccgac
      541 ggcgaccgat acgagcccac gatctcgaag tcccagttct cggcgccgtc cttccgctgc
      601 cacacggcgg agccgagcgg gagccggtcg ccgatcttcc agccctgctg cttcgcgaga
      661 tcctcgccga ccagcgctcc gttgcgcgtg tgcagcatcg cgtcgatgta ttgcggctcg
      721 atcttgatgt cggggtagat cgcgttgaag ctcgccacgt cgatggcgcc gacctggatg
      781 ctattgcggc gatcttggta gtagcccgcg aaaaaattgt agtaagacac ttcgtcgacg
      841 cccggcacgg tctgcatgcg cgcgcggtgc gcgagcggca gcggctgcgt gatgttgacg
      901 cggctcatgg tcctaagtcg cgtgtcgctc atcgcgtcga tgatgccgtc gagcgccgcc
      961 gagaagccgt gcagcaatcc gaacagcgcg aacgcgaaga cgacacagaa cagagtcagc
     1021 acggtactgg cgcgcttgcg ccatagcccg gcccagatca gcggcagata tttcatgcgg
     1081 cgactcccag cggcgcattc gtcagtgagc ccttgtcgac gtagaggcgc cgcgacgcga
     1141 aattcgccgc gcgcgggtcg tgcgtcacca tgacgatggt cttgtgctgg tgctcgttca
     1201 ggagctcgag cagcttcaag atcgattccg acgtctcgcg gtcgagatct ccggtcggct
     1261 cgtcgcagac gagcagcgtc ggatccgcga cgagcgcccg agcgatcgcg acgcgctgtt
     1321 gctggccgcc cgaaagctcg cccggcttgt gcttcgcgcg gtccgcgagg ccgacgagct
     1381 cgagcgccgc ggccacgttg cgcttgcgct ccgcggaaga gagcttcgtg agcagcagcg
     1441 gcacctcgac attgcgctcg gccgtgagaa cgggcagcag gttgtagaac tggaagatga
     1501 aaccgacgtt gcgcgcgcgc catttggcga gctgccccga cgacagctgg tcgaggcgct
     1561 cgcctgctac gacgatctcg cctcggtcgg gccggtcgag gccgccgatc aggttcagca
     1621 atgtcgtctt acccgagccc gaaggcccca tgagcgcgag gaactcgccg cgctcgaccg
     1681 tgagatcgat cgaatgcagc acctcgactc gctgcttgcc gcgcgtgtag cccttgaccg
     1741 cttgcttgat ggacaccaac gccgtcatga ctgcttcctc gtatgaggcg cgccgcgcgc
     1801 gctattcctc gaccctgacc ctcgaaccat ccgtcagcga cgacaggtcg ccactggcca
     1861 gccgcgtgcc ggccttgaga cccgcgagaa cgacctgccc tgcggcgctc tgcgggccca
     1921 ggcgcaccgt gcgccgctcg acgccgccgt cggcgatgac gaatacgacg cccgtgtcgc
     1981 cgtcggcttg cacggattcc ttcggcacca gtaccggttg ctgcgcgact gcgctcgcgg
     2041 ccgctgcatc gcgcggcgct ttcgcatcgt ggaacgccac gcgtgcgccc atctcaggca
     2101 agatccgcgg gtcgcccacc gcgaagccga cgcgtacctt cacggtagct ttcgcgcgat
     2161 cggccgtcgg aatgatcgcg atgacgtgcg ccggaatgct ccagtcggga taggcgttca
     2221 gcgtgatcgt cgccggctgg tccgtggaga cgcgctggat gaagttctcg ctgacgtcga
     2281 cctcgacctc gagcgacgac atgtcgacga tcgtgccgat acccgtccga gtgaacccgc
     2341 cgcccgccga catcggcgag accatctcgc cctcctgcgc ggctttgacg gtgacgatgc
     2401 cggcgaacgg cgcgcgcacg acggtatcgt cgagattgcg ctgcgcgagc tcgacacgtg
     2461 ccttggcgac ctcgacggcg cgcg
//
LOCUS       DASUFX010000458         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_628857,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000458 DASUFX010000000
VERSION     DASUFX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628857"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFV99_18420"
     CDS             complement(<1..899)
                     /locus_tag="VFV99_18420"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5061352.1"
                     /translation="MHDVETHRLDNGLTLHVASGHPAPVLAVQAWVGVGSADEAAPKA
                     GVAHVVEHMLFKGSSAYGVGELTRAIEGSGGEINAWTSFDHTVYHAVLGRDHLDGAID
                     AIGDALTTPRVDPHELDRERQVILEEIRQGSDDPARSVAQSLFATAYVAHPYRRPVIG
                     TPETVQRLGERDVVELIRNFYVADNITLVVAGDVDPARVRRSVERRFRAMPSGRPERR
                     VTAEPAQTSPRAVAAHRDVSEAYVSVGFHVPAARHPDVAALDVAAILLAESESARLPR
                     LLRDRDQIVTGAYAHVHALRDPG"
     gene            1035..1517
                     /locus_tag="VFV99_18425"
     CDS             1035..1517
                     /locus_tag="VFV99_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061353.1"
                     /translation="MKRLLLLAGIAALAACGSDPVNAEGTYTISVTNRDNGCNFNNWT
                     VGDSAAGINVVINQEGTDVNADVMGVTRIYLDAILGSHVYNGTVDGSDLDLDIVGTQS
                     AAMGNCTLTINSNLLASLNGDVLTGRINYHYHGNGNSDCAPYDGCTSFQEMNGTRPPQ
                     "
     gene            1522..2094
                     /locus_tag="VFV99_18430"
     CDS             1522..2094
                     /locus_tag="VFV99_18430"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5061354.1"
                     /translation="MLAIDPGAPTLSTERLVLRELRLDDARAVATRAGDRRVARFLIA
                     VPSPYPMTLATRWIAGRIAWWQQGRGVTLAIARREAPDDLLGSVSLRRFVRDRRAELG
                     YWLGADAWGQGFATEAAGALIDFGFSELVLSRIYAHVLEGNAPSCRVLDKLGMVNEGI
                     RRQHVRKGKKLLDVVLYGMLRDEWEQHQNG"
     gene            complement(2231..2530)
                     /locus_tag="VFV99_18435"
     CDS             complement(2231..2530)
                     /locus_tag="VFV99_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX5061355.1"
                     /translation="MDDREDRPTEAELDALLADARATRKKPTRAMWIAALVVSLLCVI
                     GLAYGLITSWDVEPEKGAIKAAAQTGGSGFGLGVMIGLAAGIALGSLLALRKRQS"
     gene            2645..3937
                     /locus_tag="VFV99_18440"
     CDS             2645..3937
                     /locus_tag="VFV99_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5061356.1"
                     /translation="MMRITLSLIALLAAACHYEDGKLPPDSQPDAGVDGQTGCAPGHA
                     GAACVLALYDQASASCDAADVVKLTSELDARKGIGPLWAAGRALFRTAAPIGVAGTFN
                     SWSTTALVTKAVCNSTLVVGVGAVPSGFHTYKLSDGSTWSLDAENPAFAYDDFSGNPD
                     GKNSVLDTPDSGRGHLVVLDRACSTTLGNCRDVTAYLPRGYNGTDVHAYPVLFMHDGQ
                     NVWDDHTCCFGHTGWEVNVALDAEIAAGRVAEAIVIAADNTAQRNDEYGLTPTVMNAF
                     MQFQVAELQPHALAKVRGDGRVFVAGSSLGGLVSMELALHYPQTYTGAASLSGSFWVG
                     QDDGTAVRDQLPSIGKQPLALYLDSGGSLTSNADSAADTAEVRDLLVGLGWQRGDSPN
                     CTLGPSAVCWYIEPGATHDELAWKARTWRFLRFLLPPS"
     gene            4087..4959
                     /locus_tag="VFV99_18445"
     CDS             4087..4959
                     /locus_tag="VFV99_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061357.1"
                     /translation="MRNLLALALALPLVACVVGDGTEVGDDQGGGSGSGSGSGSGSGS
                     GGGGGGISGKISSDTTWSDAVTISGNLSIDPGVTVTVSAGATITVKGGVVITVDGILD
                     AQGTSASKITIAGDNGMGFGGVSVKGELKYKYVTQTNGPMTLFTGGKATILDSHFSTV
                     AGDFLMMNGGTLDMEYSHIGIGGTALDTTHCDMHFGGAGNVIKVTHSKITSSSYGLMF
                     YGGNQADFTYNNWSNMTNVDVQASSPVSGSFNNGYFEGGAPTGTGITATGLSTAPLAN
                     CDGANDATCAGPRP"
BASE COUNT          881 a         1660 c         1674 g          834 t
ORIGIN      
        1 aagcccggat cgcgaagcgc gtgcacgtgc gcgtacgcgc cggtgacgat ctgatcgcga
       61 tcgcgaagca gccgcggcaa ccgcgccgac tcgctctcgg cgagcaggat cgctgcgacg
      121 tcgagcgcag caacatcggg atgacgtgca gccggcacgt gaaagccgac cgacacgtac
      181 gcttcgctga cgtcgcgatg cgcagcgacc gcgcgcggtg acgtctgcgc cggctcggcg
      241 gtgacgcggc gttcgggacg gcccgatggc atcgcgcgga agcgtcgctc gacgctgcgg
      301 cgcacgcgtg cgggatcgac atcgccggcg acgacgagcg tgatgttatc ggcgacgtag
      361 aagttgcgga tcagctcgac gacgtcgcgc tcgccgagcc gctgcacggt ctcgggtgta
      421 ccgatcacgg gacggcgata cggatgcgcg acgtacgcgg tcgcgaacaa gctctgcgcg
      481 accgagcgcg ccggatcgtc actgccttga cggatctctt cgaggatcac ctggcgctcg
      541 cgatcgagct cgtgcgggtc gacgcgcggc gtggtgagcg catcaccgat cgcatcgatc
      601 gcgccgtcga gatgatcgcg accgagcacc gcgtgataga cggtgtgatc gaacgacgtc
      661 cacgcgttga tctcgccgcc actgccttcg atcgcgcgcg tcagctcgcc gacgccgtac
      721 gcgctcgagc ccttgaacag catgtgctcg acgacgtggg cgacgcccgc ctttggagct
      781 gcttcatctg ctgaaccgac accaacccac gcctgcaccg cgagcactgg cgcgggatgg
      841 cctgaagcca cgtgcaacgt gagcccgtta tcgagccgat gggtctctac gtcatgcatg
      901 ccgcaacttg ccgcagggcc cgcaccgagc gagaccgcca atgcggcagc ggctcgatga
      961 taggcatggc tgcgtcggcg gggcacgttt tgggaacata ggccaactag gggatttttg
     1021 gttatcctcg acgcatgaag cgcctacttc tactcgcggg aattgctgca cttgcggcct
     1081 gtggaagcga cccggtcaac gccgagggca cgtacacgat ctcggtgacc aatcgcgaca
     1141 acggctgcaa ctttaacaac tggaccgtcg gcgacagcgc ggccggcatc aacgtcgtga
     1201 tcaaccagga aggcaccgac gtgaacgccg acgtcatggg tgtcacacgc atctacctcg
     1261 acgcgatcct cggctcgcac gtctacaacg gcaccgtcga cggcagcgat ctcgatctcg
     1321 acatcgtcgg cacgcagtcg gcggcgatgg gcaactgcac gctgaccatc aactcgaacc
     1381 tgctcgcgtc actcaatggc gacgtgctga ccggtcgaat caactatcac tatcacggca
     1441 acggcaacag cgactgcgcg ccctacgatg gctgcacgtc gttccaagaa atgaacggca
     1501 cgcgtccgcc gcagtgaacg gatgctcgcg atcgatcccg gcgcaccgac gctgtcgacg
     1561 gagcggctcg tattgcgcga gttacggctc gacgacgcgc gcgccgttgc aacgcgcgcc
     1621 ggtgatcgcc gcgtcgcacg ctttctgatc gcggtaccga gcccgtatcc gatgacgctc
     1681 gcgacgcggt ggatcgcggg ccgcatcgcg tggtggcagc aaggccgcgg ggtgacgctg
     1741 gcgatcgcgc gccgcgaagc gcccgacgat ctgctcggca gcgtcagcct gcgccggttc
     1801 gtgcgcgacc gtcgcgccga gcttggctac tggctcggcg ccgacgcgtg gggccaaggc
     1861 tttgcgaccg aagcagccgg tgcgctgatc gacttcggct tttccgagct cgtgctgtca
     1921 cgcatctacg cgcacgtgct cgagggcaac gcgccatcgt gtcgcgtgct cgacaagctc
     1981 ggcatggtca acgaaggcat tcggcgccag cacgtgcgca agggcaagaa gctgctcgac
     2041 gtcgtgctct acggcatgct ccgcgacgaa tgggagcagc accagaacgg gtgagcgagc
     2101 ggacgatatg caactcagtt gcacatggtc ctagacgtcg tgctctaaga aatggggtcg
     2161 gaccccattt ccgagcagcc agcggacgat atgcaactca gttgcatatg gtcagctgca
     2221 ccaacgagga tcaagactga cgcttgcgta gcgcgagcaa gctgccgagc gcgatgccgg
     2281 cagcaaggcc gatcatcacg ccgaggccaa agccgctgcc gcccgtctgc gccgccgcct
     2341 tgatcgcgcc cttctcgggc tcgacgtccc agctcgtgat caggccatag gcgaggccga
     2401 tcacgcagag gaggctgacg acgagcgcgg cgatccacat cgcgcgcgtt ggcttcttgc
     2461 gagtcgcacg ggcgtccgca agcagcgcgt cgagctctgc ctcggtcggg cggtcctcgc
     2521 gatcgtccac gcgtcgagcg tagcgtgcgg gatgctggag ggtacagggc gtggttcttg
     2581 ctgttcgggg cggcggcgca cgccgatccg ctagttacgg cggccacacg ttgcgtttgt
     2641 cgacatgatg cgaatcacgc tgtccctaat cgccctgctc gccgccgcct gccactacga
     2701 ggacggcaag ctcccaccgg attcacaacc cgatgccggc gtcgacggac agaccggctg
     2761 cgcgccaggt catgccggtg ccgcgtgcgt gctcgcgctc tacgaccaag cgtcggcatc
     2821 gtgcgacgcc gccgacgtcg tgaagctgac cagcgagctc gacgcgcgca aaggcatcgg
     2881 tccactgtgg gccgcggggc gcgcgctgtt tcgcaccgcc gcgccgatcg gcgtcgcggg
     2941 cacgttcaac agctggtcga cgacggcgct cgtcacgaag gcggtgtgta actcgacgct
     3001 cgtcgtcggt gtcggcgcgg tgccgagcgg ctttcacacg tacaagctca gcgatggctc
     3061 gacgtggtcg ctcgatgccg agaacccggc gttcgcatac gacgacttct ccggcaatcc
     3121 cgacggcaag aactcggtgc tcgacacacc cgactcggga cgcggtcacc tcgtcgtgct
     3181 cgatcgcgca tgctcgacga cgctcggcaa ctgccgcgac gtcaccgcgt atctgccgcg
     3241 cggttacaac ggcaccgacg tgcacgcgta tccggtgctg ttcatgcacg atggccagaa
     3301 cgtgtgggac gatcacacgt gctgcttcgg ccacaccggc tgggaagtga acgtcgcgct
     3361 cgatgccgag atcgccgccg gccgcgtcgc tgaagcgatc gtgatcgccg ccgacaacac
     3421 cgcgcagcgc aacgacgagt acggcctgac tccgacggtg atgaacgcgt tcatgcagtt
     3481 ccaggtcgcg gagctgcaac cgcatgcact cgcgaaggtg cgcggcgatg gtcgcgtgtt
     3541 cgtggccggc agctcgctcg gcggtctcgt gtcgatggag ctcgcgctgc actatccgca
     3601 gacgtacacg ggcgcggcat cgctgtcggg ctcgttctgg gtcggccagg atgacggcac
     3661 cgcggtccgt gatcagctgc cgagcatcgg caagcaaccg ctcgcgctgt atctcgactc
     3721 gggtggctcg ctgacctcga atgcagacag tgccgcggac accgccgagg tgcgtgatct
     3781 cctcgtcggt ctcggctggc agcgtggcga ctcgccgaac tgcacgctcg gaccgagcgc
     3841 ggtctgctgg tacatcgagc ccggcgcgac ccacgacgag cttgcttgga aggcccgaac
     3901 gtggcgcttc ctacggttct tgcttccgcc ctcgtaggtc cgcagcagcg ctcggggttt
     3961 gtgatccctg cccgatcggg actaactgcc ccagcgcctg ggggtaattc cctcggtttc
     4021 atcgccgtta cacgtgtcat ttcgcggtgg ttagcgggca cacgcgttgc tctatgtccg
     4081 ctcaccatga ggaacctcct agcccttgcc ctggcccttc cattggtcgc ttgcgtcgtc
     4141 ggagacggaa ctgaggtcgg agacgaccaa ggcggcggca gcgggagtgg gtcaggcagc
     4201 ggctcgggaa gcggtagtgg cggtggtggc ggcggcatca gcggcaagat cagctccgac
     4261 acgacgtgga gcgacgccgt cacgatctcg ggcaacctct cgatcgatcc gggtgtgacc
     4321 gtgacggtct ccgcgggcgc gacgatcacc gtcaagggcg gtgtcgtcat cacggtcgac
     4381 ggcatcctcg atgcgcaggg cacgagcgcc agcaagatca cgatcgccgg cgacaacggc
     4441 atgggcttcg gcggcgtaag cgtgaagggc gagctcaagt acaagtacgt cacgcagacg
     4501 aacggcccga tgacgctgtt caccggcggc aaagcgacga tcctcgactc gcacttctcg
     4561 acggtggccg gcgacttcct catgatgaac ggcggcaccc tcgacatgga gtactcgcac
     4621 atcggcatcg gaggaacggc actcgacacc acgcactgcg atatgcactt cggcggcgcc
     4681 ggcaacgtca tcaaggtgac gcactcgaag attacctcgt cgtcgtacgg cctgatgttc
     4741 tacggcggca accaggctga tttcacgtac aacaactggt cgaacatgac gaacgtcgat
     4801 gtgcaggcgt cgtcgccggt cagcggttcg ttcaacaacg gctacttcga gggtggcgcg
     4861 ccgaccggca ccggcatcac ggcgaccggc ctgtcgactg cgcccctggc gaactgcgat
     4921 ggcgcgaacg acgcgacctg cgcgggtccg cgcccgtaag cacagcgcga ctcgcgattg
     4981 tgaccccgcc cgcgtggcgg ggtttttcgg tttcggaaac aggatcagag aaaacgggtc
     5041 tgacggtct
//
LOCUS       DASUFX010000459         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_73158,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000459 DASUFX010000000
VERSION     DASUFX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73158"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFV99_18450"
     CDS             complement(<1..664)
                     /locus_tag="VFV99_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061358.1"
                     /translation="MKVRFLERLLSLGQSDELPGRLARHVRVTNALALLGLLLSLAGI
                     PLDAREALAIGLVDVVGAGMFAMCFWLNARHRYLAARIVLLLAANFVMLGGVLEVGNS
                     PEIRSSFFPLVFVPFLVLDLSERIWLALFAALPIAAYFATAQLGAAPDTLPVDVYKVY
                     APVIAFVLIISGSVVFARIDRDVDDELLRARARAIEHARLVALGELAAGVAHEIRNPL
                     AAI"
     gene            818..>3767
                     /locus_tag="VFV99_18455"
     CDS             818..>3767
                     /locus_tag="VFV99_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061359.1"
                     /translation="MRGPLLLVPAVAVACGGVPDEQHPPNQSLTFGEMVYRIIRTNLQ
                     TAQSCSLEYVSELEPHHGDFVGSFDYMLAEDIRNDLPDLLGNTILPVVQNGTLPGLVD
                     RIGESLHTLVDDQLDPQRKTLTSLVNLANAPTLIESSMVTDFASGLLAHPNLPQVLHS
                     TRILMAENDGVDVVMNDVLSLVTRSEAPSASSCTGLVLDDVQGTLLRTDGFVDDPHYA
                     LGAPGWMVRPDIHGNPKVLVDEASGKLAQPFIDLDNDGAADVGDSGLPIDVFGQRIDL
                     PYLAVIGPHDTQGRALNPHGGLLYEYYDVKRTPLSFGMQIAGDFIEAGVHHQIPAIAD
                     AVLGAPVVCNDGTSTCRAYSATNHPLADVTHLGLEMLRFPKTSKLMEVLHQLFENDPD
                     KAEDLLVAFGDVITALQNSTVTLTDSAMYDAFIGIVPLIRQIFTTSNTTGKSTPHLLV
                     DLIANMTPPEKAQIEQSLAWMVEYKSLANRPNPNPSGPVVDYRKNRFYQDAGTWVDNR
                     SGLEQAIELLAYADCGFIGCSQGSFSTSCVAATALNGFFGDPADGTVSEWLLGAMSSK
                     TPSTVSGLISFIDWLNGFSIPLVCNGAGCALEALGCSSARADDVAAHIPALRSLANSG
                     GLDWLLPIARVFDQQNQMPALVDIFDYVAADLWKSGQYDAKVDNSLSFVRRLEPPILS
                     SAKAGAIVKILGALDMLHGIQVPGTMDRASHLLVDTVDYAIANRTVNTRLGPVANSSI
                     ATELLKTMRTISSRIEAANASNALSTVVRFVTQYLTDTTTLPGGRRVLSHPNIRLMAA
                     VSLQAFADMSAITPAQQACYIDYFQQQSADYLTGRNFATLVRLAKHVTTSQNAGPVEQ
                     WLLSLLRANPTDRIEAYRPILQFSAAAASTKVADDDLQNVASWAQSLAKNNQSTALST
                     LIALDDMVQSDSNGAMVQIMRNMVAPGPATDNAAPISVFASTLGDVASIDSVNSCQAR
                     DLVTVPILEHV"
BASE COUNT          683 a         1226 c         1224 g          634 t
ORIGIN      
        1 ggatcgcggc cagcggattg cggatctcgt gcgcgacgcc tgctgccagc tcgccaagtg
       61 caacgagtcg cgcgtgctcg atggcgcgtg cgcgtgcgcg cagcagctcg tcgtcgacat
      121 cgcggtcgat gcgcgcaaac acgacggagc cgctgatgat caagacgaac gcgatgaccg
      181 gcgcatagac cttgtagacg tcgaccggga gcgtgtcggg cgcagcgcct agctgtgctg
      241 tcgcgaagta cgccgcgatc ggcagggcag cgaacagcgc caaccagatc cgctccgaaa
      301 gatcgagcac gagaaacggt acgaacacga gcgggaagaa cgaggagcgg atctcgggac
      361 tgttgccgac ctcgagaaca ccgccgagca tcacgaagtt cgccgcgagc agcaggacga
      421 tgcgcgccgc caggtagcga tggcgtgcgt tgagccagaa gcacatcgcg aacatcccgg
      481 cgcccacgac gtcgacgagg ccgatcgcga gcgcttcgcg cgcatcgagc ggtatgccgg
      541 ccagcgacag caagagaccg agcagcgcga gcgcgttcgt cacgcggacg tgccgggcca
      601 accgccccgg cagctcgtcg gactgtccaa gcgatagcag ccgttcgaga aatcgaacct
      661 tcatgcgccc cctcacgaag catcgcagat tgcttcacca accccggacg gtccggagcg
      721 cggatcttgc gccgggttcc agaccgtcat ccgtagcgcc cctgacgatt cattcctggg
      781 ttcgattttg ccgcagtgtg ttagcgtcgg ccttcggatg cgagggccat tgctgttggt
      841 tccggcggta gccgtggcgt gcgggggcgt cccggacgag cagcatccgc caaaccagtc
      901 gctgacgttc ggcgagatgg tgtaccggat catccgcacg aatctgcaga cggcacagag
      961 ctgctcgctc gagtacgtct ccgagctcga gccgcatcat ggcgacttcg tcggctcgtt
     1021 cgattacatg ctggcggagg acatccggaa cgatctgccg gacctgctcg gcaacacgat
     1081 cctgccggtc gtccagaacg gcacgttgcc ggggcttgtc gatcggatcg gcgaatcgct
     1141 gcacacgctg gtcgacgacc agctcgatcc gcagcgcaag acgctgacgt cgctcgtcaa
     1201 cctcgcgaac gcgccgacgt tgatcgagtc gtcgatggtg acggacttcg cgtcgggcct
     1261 gctcgcgcat ccgaacctgc cgcaggtgct gcactcgacg cggatcctga tggccgagaa
     1321 cgacggcgtc gatgtcgtga tgaacgacgt gctcagcctc gtcacgcgca gcgaagcgcc
     1381 atcggcgagc tcgtgcaccg gcctcgtgct cgacgacgtg caaggcacgc tgctccgcac
     1441 cgatgggttt gtcgacgatc cgcattacgc gctcggcgcg ccgggctgga tggtccgccc
     1501 ggacatacac ggcaatccga aggtgctcgt cgacgaggcc agcgggaagc tcgcgcagcc
     1561 gttcatcgat ctcgataacg acggcgcggc ggacgtcggc gatagcgggc tgccgatcga
     1621 cgtgttcggt cagcggatcg atctgccgta tctcgcggtg atcggaccgc acgacacgca
     1681 gggtcgcgcg ctcaacccgc acggcggcct cttgtacgag tactacgacg tcaaacgcac
     1741 gccgctgtcg ttcggcatgc agatcgccgg ggatttcatc gaggccggcg ttcatcatca
     1801 gattccagcg atcgcagacg cggtgctcgg tgccccggtc gtgtgcaacg acggcacgtc
     1861 gacgtgtcgc gcgtactcgg ccacgaatca tccgctcgcc gacgtgaccc acctcggtct
     1921 cgagatgctg cggttcccga agacgtcgaa gttgatggag gtgctccatc agctgttcga
     1981 gaacgacccc gataaggcag aggacttact cgtcgcgttc ggcgatgtga tcacggcgct
     2041 ccagaattcg acggtcacgc tcactgactc ggcgatgtac gacgcgttca tcggcatcgt
     2101 gccgctgatt cgccagatct tcactaccag caacacgacc ggcaagtcga cgccgcacct
     2161 gctcgtcgat ctgatcgcga acatgacgcc tcccgagaag gcgcagatcg agcagtcgct
     2221 cgcgtggatg gtcgagtaca agtcgctcgc gaaccggccc aatccgaacc cgagcggtcc
     2281 ggtggtcgac tatcgcaaga accgcttcta tcaggatgcc ggcacgtggg tcgacaaccg
     2341 cagcggcctc gagcaggcga tcgagctgct agcgtatgcg gattgcggct tcatcggctg
     2401 ttcgcagggc tcgttctcga cgagctgtgt cgcggcgacc gcgctcaacg gcttcttcgg
     2461 cgatcctgcc gacggcacgg tgtcggagtg gctgctcggc gcgatgtcga gcaagacgcc
     2521 gtcgacggtt tcggggctga tctcgttcat cgactggctc aacggcttct cgattccgct
     2581 cgtgtgcaac ggtgcgggct gcgcgctcga agcgctcggc tgcagctcgg cgcgcgctga
     2641 tgatgtcgcg gcgcacattc cggcgctgcg cagcctcgcg aactcgggcg gcctcgactg
     2701 gctgctgccg atcgcccgcg tgttcgacca gcagaatcag atgccggcgc tcgtcgacat
     2761 cttcgactac gtcgcggccg atctatggaa gagcggccag tacgacgcaa aggtcgataa
     2821 ctcgctcagc ttcgtgcgcc ggctcgagcc gccgatcctg tcgtcggcga aggcgggtgc
     2881 gatcgtgaag atcctcggcg cactcgacat gctgcacggt atccaggtgc caggcacgat
     2941 ggatcgcgcg agccacttgc tcgtcgatac cgtcgactac gcgatcgcga atcgcaccgt
     3001 gaacacgcgg ctcggcccgg tggcgaacag ctcgatcgcg accgagctgc tcaagacgat
     3061 gcgcacgatc agctcgcgca tcgaggcggc gaacgccagc aacgcgctgt cgacggtggt
     3121 tcgcttcgtg acgcagtatc tgaccgacac gacgacgctg ccgggcggcc ggcgcgtgct
     3181 gtcgcatccg aacatccgac tcatggccgc cgtcagtctg caggcgttcg cggacatgtc
     3241 tgcgatcacg ccggcgcagc aggcttgcta catcgattac ttccagcagc agagcgctga
     3301 ctacctgacc ggtcgcaact tcgcgacgct cgtacgcctc gcgaagcacg tgacgacgtc
     3361 gcagaacgca ggaccggtgg aacagtggtt gctgtcgctg ctccgcgcga acccgacgga
     3421 tcgcatcgag gcgtatcggc ccatcctgca gttctcggcg gcggcagcgt cgacgaaagt
     3481 cgccgatgac gatctccaga atgtcgcgtc gtgggcgcag tcgctcgcga agaacaacca
     3541 atcaacggcg ctctcgacgc tgatcgcgct cgacgacatg gtgcagagcg acagcaacgg
     3601 cgcgatggtc cagatcatgc gcaacatggt cgccccgggg ccggccaccg ataatgcggc
     3661 accgatctcg gtgttcgcgt cgacgctcgg cgatgtggcg agcatcgaca gcgtgaattc
     3721 atgccaggca cgcgatctcg tgacggtgcc gattctcgag cacgtcg
//
LOCUS       DASUFX010000460         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_73354,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000460 DASUFX010000000
VERSION     DASUFX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73354"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="VFV99_18460"
     CDS             <1..1922
                     /locus_tag="VFV99_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX5061360.1"
                     /translation="TLVVQGGLSTSTEVLAMETKSLDRVVIRFAGDSGDGMQLTGTEF
                     AKAAAEAGNDISTFPDFPAEIRAPAGSLFGVSGFQLHFSSSEIHTPGDAPDVLVCMNP
                     AALKTNLKDLVDTGTLIINTGAFIEANLKKAGYQTNPLEDGSLDKYKVHAVDISHLTT
                     TALEGTELSTKEKGRSKNFFALGLVFWMYGREPEAEIRRIYQQFSKKPGFGEANVKVF
                     KAGYHYGETAEVFQTVYKVPPATFTPGMYRNITGNEAIALGLVVGAELAGKKLFYSGY
                     PITPASSILHSLAKYKNFGTMTFQAEDEIAAMGAALGAAYGGSIAVTASSGPGIALKG
                     EAIGLGVMTELPVVILNIQRGGPSTGLPTKTEQSDLSQSLYGRNGESPVPVLAAKSPG
                     DTFYCAIEAVKIATRYMTPVILLSDGYIANGSEPWLLPNLAALPRFDVIHRTDPNGYF
                     VYQRDERNLARAWVVPGTPGLEHRIGGIEKDALTGNISYAPDNHEKQTRIRQEKVERV
                     AQDIGELDLSGGESGDVLIIGWGGTYGALRQAQQALAAQGKKVSHAHCRWLMPLEPGV
                     AKIIHNFKHVIVAELNMGQLRAVIREKFLVDAVGLNKIQGQPFKVREVMAAIDKQLEA
                     VPANASSSQQIQVNA"
     gene            1919..2944
                     /locus_tag="VFV99_18465"
     CDS             1919..2944
                     /locus_tag="VFV99_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX5061361.1"
                     /translation="MSEAVKIKLSKKDLESDQDVRWCPGCGDYAILATVQRTLASLGV
                     KRESTVFISGIGCSSRFPYYMNTYGFHTIHGRAPAIASGLKVTRPELDVWVITGDGDG
                     LSIGGNHMIHALRRNMDLKIILFNNQIYGLTKGQYSPTSALGTRAPSTPSGSIDHPLN
                     PIALALGAGATFIARAADTDAKGMGKILEEAYKHKGAVFIEMLQNCPVFNDGVWEGVK
                     EDPANRQIALVDGKPLTFAGGTKGIKLGAGLVPEIVDVKDGNTSELLVHTERGSTLYA
                     NLLSQLVQPSFPMPMGILYRHDKPTYEQLAISQVQEAIEKQGPGDLNKLMYSGMVWEV
                     GADGTRH"
     gene            2974..>3976
                     /locus_tag="VFV99_18470"
     CDS             2974..>3976
                     /locus_tag="VFV99_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061362.1"
                     /translation="MTAIGACTTGAPPGFSDGEQWVIPLVGPLEDGLLLVPTFVNDQG
                     PFLFAIDPDAYISIIDEDVLHTTKVPAGEGPHLLDESDTQQTRQYAEVSSLKAGTLTV
                     SGQRYVQLVRKGTFDADGRRIHGVLGRDVIADSLVFGFDRDLGVATLSTQKAFKKPVG
                     AMSLSYTKLLSTVLNAEVVPLPRRLVHATIGGMQYPLHVDFGATYSQLRPRAWPRANL
                     ATSETQTGVVDEVGVIRPVKQMGVADEVTVRDATTKSVSFVPYADKRWPDQDIEGTLG
                     LGFFRPYSVFANWDNDTLYLTPRKDGTSTKLVARIGRWQSKTLSGCADVGCVKVSLVD
                     "
BASE COUNT          756 a         1291 c         1286 g          643 t
ORIGIN      
        1 gcacgctcgt cgttcagggt ggtctgtcga cgagcacaga ggttttagcg atggaaacga
       61 agagtctgga ccgagtcgtg attcgcttcg cgggcgactc gggcgatggc atgcagctca
      121 ccggcaccga gttcgcgaaa gcggcggctg aagccggaaa cgatatctcg accttccctg
      181 acttccctgc agagattcgc gcgccggccg gcagcttgtt cggcgtctcg ggattccagc
      241 tgcacttctc gtcgtcagag attcacacgc ccggcgatgc acccgatgtg ctcgtgtgca
      301 tgaacccggc ggcgctcaag acgaacctca aagatctcgt cgacaccggc acgctgatca
      361 tcaacaccgg cgcgttcatc gaggcgaacc tcaagaaggc cggctatcag acgaacccgc
      421 tcgaagatgg cagcctcgac aagtacaagg tccacgccgt cgacatcagt cacctgacga
      481 cgaccgcgct cgagggcacc gagctctcga cgaaggagaa gggccgcagc aagaacttct
      541 tcgcgctcgg cctcgtgttc tggatgtacg gccgcgagcc cgaagcggag attcgccgca
      601 tctaccagca gttctcgaag aagccgggct tcggcgaagc gaacgtgaag gtgttcaagg
      661 ctggctatca ctacggcgag accgccgaag tgttccagac ggtctacaag gttccgcccg
      721 cgacgttcac gccgggcatg taccgcaaca tcaccggtaa cgaagcgatc gcgctcggcc
      781 tcgtcgtcgg cgcggagctc gccggcaaga agctgttcta ctcgggctat ccgatcacgc
      841 ccgcgtcgtc gatcctgcac tcgctcgcca agtacaaaaa cttcggcacg atgacgttcc
      901 aggccgagga cgagatcgcg gcgatgggag ctgcactcgg cgcggcgtat ggcggttcga
      961 tcgcggtcac cgcgtcgtcg ggcccgggca tcgcgctcaa aggcgaagcg atcggtctcg
     1021 gcgtgatgac cgagctcccg gtcgtgatcc tgaacatcca gcgcggcggt ccatcgaccg
     1081 gtctgccgac gaagaccgag cagagcgacc tctcgcaatc gctgtatggc cgcaacggcg
     1141 aatcgccggt gcccgtgctc gcggcgaagt cgcccggcga cacgttctac tgcgcgatcg
     1201 aggccgtgaa gatcgcgacg cgttacatga cgccggtgat tctgctcagc gacggttaca
     1261 tcgcgaacgg ctccgagccg tggctgctgc cgaacctcgc agcgctgccg cgcttcgacg
     1321 tcattcatcg caccgatcca aacggctact tcgtgtacca gcgtgacgag cgcaatctcg
     1381 cgcgcgcgtg ggtcgtgccc ggcacgccag gtctcgagca ccgcatcggc ggcatcgaga
     1441 aggacgcgct caccggcaac atctcgtacg cgcccgacaa ccacgagaag cagacgagga
     1501 tccgccagga gaaggtcgag cgcgtcgcgc aagacatcgg cgagctcgat ctctcgggtg
     1561 gcgagtccgg cgacgtgctg atcatcggct ggggcggcac gtacggtgcg ctccgtcagg
     1621 cgcagcaggc gctcgccgcg caaggcaaga aggtgagcca cgcgcactgt cgctggctga
     1681 tgccgctcga gcccggcgtc gcgaagatca tccacaactt caagcacgtc atcgtggccg
     1741 agctcaacat gggccagcta cgtgccgtga ttcgcgagaa gttcctcgtc gacgcggtcg
     1801 gcctcaacaa gatccaaggc cagccgttca aggtccgcga ggtgatggcc gcgatcgaca
     1861 agcaactcga agcagtgccg gcgaacgcgt cgtcgtcgca gcagatccag gtgaacgcat
     1921 gagtgaagca gtcaagatca agctgtcgaa gaaggacctc gagtccgatc aggacgtgcg
     1981 ttggtgcccg ggttgcggtg actacgccat cctcgcgacg gtacagcgca cgctcgcttc
     2041 gctcggcgtc aagcgcgaga gcacggtgtt catctccggc atcggctgct cgagccgctt
     2101 cccgtattac atgaacacct acggcttcca cacgatccac ggccgcgcgc cggcgatcgc
     2161 gtcgggcctc aaggtcacgc gccccgagct cgatgtctgg gtgatcaccg gcgacggcga
     2221 cggcctgtcg atcggcggca accacatgat ccatgcgctc cgccgcaaca tggatctcaa
     2281 gatcatcctg ttcaacaacc agatctacgg tctgacgaag ggccagtact cgccgacgag
     2341 cgcactcgga acgcgcgcac cgtcgacacc gagcggctcg atcgatcacc cgctcaaccc
     2401 gatcgcgctc gcgctcggcg ccggcgccac gttcatcgcg cgcgcggccg acaccgacgc
     2461 gaaggggatg ggcaagatcc tcgaggaggc ctacaagcac aagggtgcgg tcttcatcga
     2521 gatgctgcag aactgccccg tcttcaacga cggcgtgtgg gaaggcgtca aagaggatcc
     2581 ggcgaatcgc cagatcgcgc tcgtcgatgg caagccgctg acgttcgcgg gtggcacgaa
     2641 gggcatcaag ctcggtgcgg gcctcgttcc cgaaatcgtc gacgtcaagg acggcaacac
     2701 gtcggagctg ctcgtgcaca ccgagcgcgg ctcgacgctc tacgcaaatc tgttgtcgca
     2761 gctcgtgcaa ccgagcttcc cgatgccgat gggcatcctg tatcgccacg acaagccgac
     2821 gtacgagcag ctagcgatca gccaggtcca ggaagcgatc gagaagcagg gtccgggcga
     2881 cctcaacaag ctgatgtact cggggatggt gtgggaagtc ggcgcggacg gcacgcggca
     2941 ctagtcgcgc gcgcgctcct cggagcgtgt gcggtgaccg cgatcggcgc ctgcacgaca
     3001 ggcgcaccgc cggggttctc ggacggcgag cagtgggtga tcccgctcgt cggtccactc
     3061 gaggacgggc ttctgctcgt cccgacgttc gtcaatgacc agggcccgtt cctgttcgcg
     3121 atcgatcccg atgcgtacat ctcgatcatc gatgaggacg tgctgcacac gacgaaggtg
     3181 cctgcgggcg agggtccgca cttgctcgac gagagcgaca cccagcagac gcgccagtac
     3241 gccgaggtat catcgctcaa agccggcacg ctgaccgtca gcggccagcg gtacgtgcag
     3301 ctcgtccgca agggtacgtt cgacgccgac ggtcgccgca ttcacggcgt gctcggtcgc
     3361 gacgtgatcg ccgactcgct cgtgttcggc ttcgaccgcg atctcggtgt cgcgacgctg
     3421 tcgacgcaaa aggcattcaa gaagccagtc ggcgcgatgt cgctctcgta tacgaagctc
     3481 ctgagcaccg tgctcaacgc ggaagtcgtt ccgctgccgc ggcggctcgt gcacgcgacg
     3541 atcggcggca tgcagtatcc gctgcacgtc gacttcggtg cgacgtacag ccagctgcgc
     3601 ccgcgcgcct ggccacgtgc gaacctcgcg acgtcggaga cacagaccgg cgtcgtcgac
     3661 gaagtgggcg tgattcgccc ggtgaagcag atgggcgtcg ccgacgaggt gaccgttcgc
     3721 gatgcgacga cgaagagtgt cagcttcgtg ccgtacgccg acaagcgctg gcccgatcag
     3781 gacatcgagg gcacgctcgg cctcggcttc ttccggccgt attcggtgtt cgcgaactgg
     3841 gacaacgaca cgctgtacct gacgccgcgc aaggacggta cgagcacgaa gctcgtcgcg
     3901 cgcatcgggc gctggcagtc gaagacgctg agcggctgtg cggatgtcgg ttgcgtgaag
     3961 gtgagcctcg tcgatc
//
LOCUS       DASUFX010000461         8343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_73433,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000461 DASUFX010000000
VERSION     DASUFX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73433"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFV99_18475"
     CDS             <1..371
                     /locus_tag="VFV99_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5061363.1"
                     /translation="TPQAYRCGQVWGIHIEVAGMRFYHQGSADFLEDEIKDKNVDVFL
                     CGISGRRFTPNYVERIVKKLAPNVIVPTHYDDFFRRLDDKVQFSFNVNLTGFADEVRQ
                     ASRDLPLHALELGVTCRTTG"
     gene            353..934
                     /locus_tag="VFV99_18480"
     CDS             353..934
                     /locus_tag="VFV99_18480"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX5061364.1"
                     /translation="MPNYWLMKTEPDTFSIDDLERVKVEPWTGVRSVFARFHMRNMSV
                     GDEVLFYHSSCEPPGVAGLARVVQTKVIDETQFDPQSKYFDEKATREKPIWDCVAVEF
                     VEKLPYLVSLPHIRAEPALKDMKLLQIGRLSVMPVAEAEYKLIALMGHVEPKPLPVQK
                     KVARPKVKAKPKAAKAAKTAKKRLPTPAKKHKR"
     gene            931..1329
                     /locus_tag="VFV99_18485"
     CDS             931..1329
                     /locus_tag="VFV99_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061365.1"
                     /translation="MSDDVRKLMDEAYAHDRKGDERGAIKFYDAAYRAGVPESERRKF
                     LVGYGSTLRNVGRAEDAVAILGEAIAADPDYPAFTAFLALALADAGHPKAALAAMLGC
                     ALDVARPDAFGGYERALTEYHQLLLEKSQS"
     gene            complement(1299..2126)
                     /locus_tag="VFV99_18490"
     CDS             complement(1299..2126)
                     /locus_tag="VFV99_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061366.1"
                     /translation="MRFELHCHSTCSDGTEAPEQVAARASERAVDVFALTDHDTCAGS
                     AVQVGTARVLRAVEISCDEPDTGRTIHVLAYDRGGAAWSDLEQRLGDLRLARRNRMRM
                     MAARLAQRGIRVDVEPMIAEAEQSGRTLGRPDLARLMVAAGAVNTIKDAFTRHLYDNG
                     PVDVPHHALTLVDALALGRAAGAAMSLAHPHLYDQLGVALLRKHRGDGLAGVEAFYGV
                     YDVHERKRWVDVANELGLVCTAGSDFHYAEDGMVPIGVDVDGDRGKQLYDWLFSSNS"
     gene            2211..4046
                     /locus_tag="VFV99_18495"
     CDS             2211..4046
                     /locus_tag="VFV99_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061367.1"
                     /translation="MPIPGPQAQARLGRYELLARLATGGMGEIFLARLSGAEGFQKLY
                     VVKRILAHLADDARFRQMLVAEAQIAAKMSHPNICQVFELGETDGQLYIVMEYLEGVS
                     LLPLLRRFSKENTALDLGFVAGVLQQVTDAMNYAHDLKERDGDFLGIIHRDVTPSNIF
                     LTETGVAKVLDFGIAKARGASTQTQEGTVKGKYAYMAPEQLKGNSIDRRVDIFALGVV
                     LYEMLALRRLFQRKTDYLTFRAVMEQPIPDIRRYRPDLPDAVADALTRALERDPNNRY
                     ETARQLGTALLDGMQGTRPWTQGEISDFVRHYFAEEIGKRSQQVASVVARTQSGVASS
                     RATMPLLIHPDEGMHEGDGEDDFPSVETEVESAPLWMPRPKPPNAGASSDFAGSSTSG
                     TPQPFGIESTGSAPSLQPIRGQQPTPGPNQVVVVPRRSVFWPIFGVLMVVIAGGALFL
                     VWQQMQKQQTPAEIKITQESAAKPNEEPVETHTIDSQPQDQGSSEVPPPAGSGSSTAG
                     PGSGERKPPKPKSFAEQINAAMAARKPELLSCINSHPDGIPKGKAIEASISFDAKGHT
                     TSVSLSPADVNASPLGMCLKNAVKKAAFPNGRETSFSVPLTPKGA"
     gene            complement(4136..5296)
                     /locus_tag="VFV99_18500"
     CDS             complement(4136..5296)
                     /locus_tag="VFV99_18500"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX5061368.1"
                     /translation="MTRRLYHDDAYLRRFDAEVVAHATYKDQPALVLDQTAFYPEAGG
                     QLGDRGTLAGLAVSDTQELDDGRIAHVVGGDLPAIGARVNGELDWARRRQHMAQHTAQ
                     HLLSGTLLDRAQAPTASARLGETSLTIDAARDRIPDAELAAAEDLANDLIDDDLAIRA
                     WFPSADELAAIKLRRDPKVTADIRIVAIGDFDFSPCGGTHCARTSQLGSIRITGSERY
                     KGMTRVTFTAGRRARVELFTRDQVLRGLATQFSCGPAEVPAAIDKLRRDVDSSSTEIT
                     NLRSRLATLLVDSFAGTGAVIAAIPGDGELMRSVAAKLVGAGRDALLSVADDTGTHVI
                     LMRAQGSALDCGALWKKLAAQLGGRGGGRADRAEGRVPTRVADWAETIRPLL"
     gene            complement(5309..7816)
                     /locus_tag="VFV99_18505"
     CDS             complement(5309..7816)
                     /locus_tag="VFV99_18505"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX5061369.1"
                     /translation="MKRLAIGILVLLGLAGIASAEEPRAELRLESQPHAGVPFNIAMV
                     IAGFDEQPQPQQPKLDIPGARVTPLGGEPSVSQSIQIINGKRSDFKRVTWTLRWRVEL
                     DKAGRVRVPAVTVVQGSKRVTTQAGDLDAEAVPTTDDMKIELALPDRTVFVGETIEAT
                     LIWRFRTEPESQTFSVPLMSMDEFIISAPPATDPRRALSLPAGSKELKLPYDVADDGA
                     FKKVTVRFYVAPKKPGKLEVPGPTVVAALAVGRRDFFGNAPTRMFRTSGAAKTLEVKP
                     LPETDKPPGFAGAVGSQFSIAVATSRSVVQLGEPVELTITVKSNQRLDTLSLGHLDAP
                     GGLPKDKFTVPAEAPTGELADDGLSKTFKVTAQVTGPATEIPAIAFSYFDPVKGHYQT
                     IHSDPIAVSVKGGSIVGAGDVVAMSPKHGSHAANLPEADLALVGADLALSAPGVVDSR
                     PLGGALLWLLIAGLYAIPLALFGVRSWQLRTQTQREEAAEVKAARKKVEHELARAAKD
                     PARDTAGPLVAALRAFARVLAIPEREPRDSVLADIEHAGYAPSSATSPLSAELRTRAE
                     DLMKRWTAETKHKRSPNATAAAVLLAIVLAPAASHASPLDDGRTAYQQAMTLTDASAR
                     KAAFTRAAAALGDAARTTPDRPELLTDWGNAALGAGDVATATLAYRRALALDGGNARA
                     RRNLAWLRSKLPDTMRPAEGSATDSLFFFHHWPRSSRLLVGAVAFGIAILLLVPWAGR
                     RRRALTGISVLPFAVWLAMLLSLVLEDRHADDAIVMDGVVLRAADSAGAPAALTQPLP
                     RGAEVRLVERRDTWTKIQLASGTTGWVPDGAVQRISR"
     gene            complement(7816..>8343)
                     /locus_tag="VFV99_18510"
     CDS             complement(7816..>8343)
                     /locus_tag="VFV99_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061370.1"
                     /translation="TAHTEDDETRAPKLPGIASRQDQHGQLAKAITDALAQQADAAAK
                     AQDPQQQQQGKNMAAAADEVRFAQNDMADAKRTLDRAVQTTNQSFSLEPTIKSQASAI
                     EHLMNALKLLQPPQQQQQDKDKQQQQQQQDKDKKQEQQPQQGGAGQRARDEDARRQRE
                     RKEQESGGDTVEKDW"
BASE COUNT         1338 a         2752 c         2849 g         1404 t
ORIGIN      
        1 tgacgccgca ggcgtatcgc tgcggccagg tctggggcat ccacatcgag gtcgccggga
       61 tgcgcttcta tcatcaaggg tcggccgatt tcctcgagga cgagatcaag gacaagaacg
      121 tcgacgtgtt cttgtgcgga atctccggcc gccggttcac gccaaactac gtcgagcgca
      181 tcgtcaagaa gctcgcgccg aacgtgatcg tgccgacgca ctacgacgac ttcttccgcc
      241 ggctcgatga caaggtgcag ttctcgttca acgtgaacct gaccggtttc gctgacgagg
      301 tacgccaggc ctcgcgcgat ctaccgctcc acgcgctgga gctcggagtg acatgccgaa
      361 ctactggctg atgaagaccg agcccgacac gttcagcatc gacgacctcg agcgcgtgaa
      421 agtggagccg tggaccggcg tgcgcagcgt gttcgcgcgc tttcacatgc gcaacatgag
      481 cgtcggcgac gaagtgctgt tctatcactc gagctgcgag ccgccgggtg tcgccggcct
      541 cgcgcgcgtc gtgcagacaa aggtgatcga cgagacgcag ttcgatccac agtcgaagta
      601 cttcgacgag aaggcgacgc gcgagaagcc gatctgggat tgcgtcgcgg tcgagttcgt
      661 cgagaagctg ccatatctgg tctcgctgcc gcacattcgc gccgagccgg cgctgaagga
      721 catgaagctg ttgcagatcg gccggctgtc ggtgatgccg gtcgccgaag ccgagtacaa
      781 gctgatcgcc ctgatgggcc acgtcgagcc aaagccgctg cctgtgcaga agaaggtcgc
      841 gcgacccaag gtgaaggcga aaccaaaagc cgcgaaggcc gcgaagaccg ccaagaaacg
      901 gctccccact ccggcgaaga agcacaagcg atgagcgatg acgttcgcaa actgatggac
      961 gaggcctacg cgcacgatcg caagggcgac gagcgtggtg cgatcaagtt ctacgacgcg
     1021 gcgtatcgcg ccggcgtgcc ggagagcgag cggcgcaaat tcctcgtcgg ttacggatcg
     1081 acgctacgca atgtcggacg cgccgaggat gccgtcgcga ttctcggcga ggcgatcgcg
     1141 gccgaccccg actatccggc gttcaccgcg tttctcgcgc ttgcgctcgc cgacgccggg
     1201 catcccaagg cagcgctcgc cgcgatgctc ggctgcgcgc tcgacgtcgc gcggcccgac
     1261 gcgttcggcg gctacgagcg cgcgctcacc gaatatcatc aactgttgct cgagaagagc
     1321 cagtcgtaga gctgcttgcc gcgatcgccg tcgacgtcga cgccgatcgg caccatgccg
     1381 tcctcggcgt aatggaagtc tgaccccgcg gtgcacacga ggccgagctc gttcgcgacg
     1441 tcgacccagc gcttgcgctc gtgaacatca tagacgccgt agaacgcctc gacgccggcg
     1501 agcccgtcgc cgcgatgctt gcggaggagc gcgacgccga gttgatcgta gagatgtgga
     1561 tgcgcgagag acatcgcggc gcccgctgca cggccgagcg cgagcgcgtc gacgagcgtc
     1621 agcgcgtggt gcggaacgtc gaccggcccg ttgtcataga gatggcgcgt gaatgcgtct
     1681 ttgatcgtgt tgaccgcgcc ggccgcgacc atgagacgcg cgaggtccgg tcggccgagc
     1741 gtgcgtcccg attgttcggc ctccgcgatc atcggctcga catcgacccg gatgccacgt
     1801 tgcgcgaggc gcgcggccat catccgcatg cgattgcggc gcgcgagccg aagatcgccg
     1861 agccgctgct ccaggtctga ccaggcggcg ccgccacgat cgtacgcgag cacgtggatc
     1921 gtgcgcccgg tgtcgggctc gtcgcacgaa atttccaccg cacgtaaaac gcgagcggtg
     1981 ccaacctgga cagcagaacc tgcgcacgtg tcgtgatcgg tgagcgcgaa gacgtcgacg
     2041 gcgcgttcac tggcacgcgc ggcgacttgc tcgggcgctt cggtaccgtc ggagcatgtc
     2101 gaatggcagt gcagctcgaa ccgcatcgtc gtcggaccct agcacgtggg cagatgtagg
     2161 cgcgccccac gccggtatcg tcaagctcgc tctgctacca tccaattttg atgcccatcc
     2221 cgggtccaca ggcgcaagca cgtctcggcc gttacgagct gcttgcgcgt ttggcgacgg
     2281 gcggcatggg cgaaatcttt ctcgcgcggc tctcgggcgc ggaaggattc cagaagctgt
     2341 acgtcgtcaa gcgcatcctc gcgcacctcg cggatgacgc gcgcttccgt cagatgctcg
     2401 tcgccgaagc gcagatcgca gcgaagatga gccacccgaa catctgtcag gtgttcgagc
     2461 tcggcgagac cgacggccag ctctacatcg tgatggagta cctggagggc gtctcgctgt
     2521 tgccgctgct caggcgattc tcgaaggaga acaccgcgct cgatctcggc tttgtcgccg
     2581 gtgtgctgca acaggtcacc gacgcgatga actacgcgca cgacctgaag gagcgcgacg
     2641 gcgactttct cggcatcatc catcgcgacg tcacgccgtc gaacatcttc ctcaccgaga
     2701 ccggcgtcgc gaaggtgctc gacttcggca tcgcgaaggc gcgtggagcg tcgacgcaga
     2761 cccaagaagg cacggtcaag ggcaagtacg cgtacatggc gcccgagcag ctcaagggca
     2821 acagcatcga tcggcgcgtc gacatcttcg cgctcggcgt cgtgttgtac gagatgcttg
     2881 cgctgcgccg gttgttccag cgcaagaccg attacctgac gtttcgcgcg gtgatggagc
     2941 agccgattcc agacatccgc cgctaccgtc cggatctgcc ggatgcagtc gccgacgcgc
     3001 ttacccgcgc gctcgagcgc gatccgaaca atcgttacga gaccgctcgc cagctcggta
     3061 cggcgctgct cgacggcatg cagggcaccc ggccctggac ccagggcgag atcagcgact
     3121 tcgtgcgcca ctacttcgcc gaagagatcg gcaagcgcag ccagcaggtc gctagcgtcg
     3181 tcgcgcgcac gcagtcgggt gtggcgtcgt cgcgtgcgac gatgccgctg ctcattcatc
     3241 ccgacgaggg catgcatgaa ggcgacggcg aggacgattt cccgtcggtc gagaccgagg
     3301 tcgaaagtgc gccgctgtgg atgccgcgcc cgaagccgcc caacgctggc gcgagcagcg
     3361 atttcgccgg cagctcgacg agcgggacac cgcagccgtt cggtatcgag tcgaccggca
     3421 gcgcgccgag cttgcagccc atccgtggcc agcagcccac gccggggccg aatcaggtcg
     3481 tcgtcgtgcc acggcgcagc gtgttctggc cgatcttcgg cgtgttgatg gtcgtgatcg
     3541 cgggcggcgc gctgttcctg gtgtggcaac agatgcagaa gcagcagacg ccggcggaga
     3601 tcaagatcac gcaggagtcg gcggcgaagc cgaacgaaga gccggtcgag acgcacacga
     3661 tagactcgca gccgcaggat caaggcagct ctgaggtgcc accaccggct ggctcgggct
     3721 cgagcaccgc gggccctggc agtggtgagc gaaagccgcc gaagccgaag tcattcgccg
     3781 agcagatcaa cgcggcgatg gccgcgcgca agcccgagct cttgagctgc atcaactcgc
     3841 atcccgacgg cattccgaag ggcaaggcga tcgaggcatc gatcagcttc gatgcgaagg
     3901 gccacacgac gtcggtctcg ctgtcgccgg ctgacgtcaa cgcgtcgccg ctcggcatgt
     3961 gcctcaagaa cgccgtgaag aaggccgcct ttccgaacgg ccgcgagacg tcgttctcgg
     4021 tcccgctgac gccgaagggc gcctagctcg aattgtcctt cgtcgcggac agcacctctt
     4081 cgatgcggcg cgcgggcgcc agcgcacggt gacgcgggcg cgcacgcaca tcgtgctaga
     4141 gcagcggccg gatcgtctcg gcccagtcgg cgacgcgagt cggcacgcgg ccttccgcgc
     4201 gatcggcccg accaccgccg cggccgccga gctgcgctgc gagcttcttc catagcgcgc
     4261 cgcagtccag ggccgaacct tgggcgcgca tcaagatgac gtgcgtgccg gtgtcgtcgg
     4321 cgacactgag gagcgcgtct cgcccggcgc cgaccagctt ggcggcgaca ctgcgcatca
     4381 gctcgccgtc gccgggaatc gccgcgataa cggcgccggt gccagcgaac gagtcgacga
     4441 gcagcgtcgc gagccggctg cggaggttcg tgatttcggt gctcgagctg tcgacgtcgc
     4501 gccggagctt gtcgatcgct gcgggaactt cggcagggcc gcacgagaac tgcgttgcca
     4561 ggccgcgcag cacctgatcg cgcgtgaaca gctcgacgcg tgcgcgtcgg ccagccgtga
     4621 acgtcacacg cgtcatgcct ttgtagcgct cgctgcctgt gatgcggatc gaaccgagct
     4681 gcgaggtgcg cgcgcagtgc gtgccgccgc acggcgagaa atcgaaatca ccgatcgcga
     4741 cgatgcgaat gtcggcagta accttcggat cgcggcgcag cttgatcgcg gcaagctcgt
     4801 ccgcgctcgg gaaccaggcg cgaatcgcga ggtcatcgtc gatcagatcg ttggccaggt
     4861 cttcggctgc ggcgagctcg gcatcgggaa tccggtcgcg cgccgcatcg atcgtcagcg
     4921 aggtctcgcc gagtcgcgcc gatgccgttg gcgcctgtgc gcgatcgagc aacgtgcccg
     4981 acagcaggtg ctgcgcggtg tgctgcgcca tgtgctggcg gcggcgggcc cagtcgagct
     5041 cgccgttgac ccgcgcgccg atcgccggta ggtcgccgcc tacgacgtgc gcgatgcggc
     5101 catcgtcgag ctcctgcgta tcactgacag cgaggccggc gagcgtgccg cgatcgccga
     5161 gctgcccacc ggcctcggga taaaacgcag tctgatcgag aaccagcgct ggctgatcct
     5221 tgtatgtcgc gtgcgcaacg acctccgcat cgaagcggcg gaggtaggca tcgtcgtgat
     5281 agaggcggcg ggtcacagcc cgcgcagctt agcgtgaaat tcgctgcacg gcgccgtcgg
     5341 gaacccatcc ggtcgtgccg ctggcgagct gaatcttcgt ccacgtatcg cgccgctcta
     5401 cgagccggac ttcggcgcca cgcggcaacg gctgtgtaag cgcagcgggg gcgcccgcgc
     5461 tatcggctgc gcgcagcacg acaccatcca tcacgatggc atcatcggcg tggcggtctt
     5521 cgagcacgag cgacagcagc atcgcgagcc acaccgcgaa tggtagtacg gagattcctg
     5581 tcagcgcgcg gcggcggcgg cccgcccacg gcacgagcaa caggatcgcg atgccgaacg
     5641 cgaccgcgcc gacgagcaag cggctcgagc gcggccagtg atgaaagaag aacaacgagt
     5701 cggtcgcgct gccttccgcg ggccgcatcg tgtcggggag cttgctgcgc agccacgcca
     5761 ggttccggcg cgcgcgtgcg ttaccgccgt cgagtgcgag tgcgcggcga taggcgagcg
     5821 tcgcggtcgc gacatcaccc gcaccgagcg ccgcgttgcc ccagtccgtg agcagctcgg
     5881 gacggtcggg cgtggtgcgc gcggcgtcgc cgagcgcagc ggctgcgcgc gtgaacgccg
     5941 ccttgcgcgc gcttgcatcg gtgagcgtca tcgcttgctg atacgcggtg cggccgtcgt
     6001 cgagcggtga cgcgtggctc gccgcaggcg cgaggacgat cgcgagcaac acggctgcag
     6061 cggtcgcatt cggcgagcgc ttgtgcttgg tttctgccgt ccaccgcttc atcagatctt
     6121 cggcgcgcgt gcgcagctcg gccgagagcg gcgacgtcgc cgagctcggt gcgtagccgg
     6181 cgtgctcgat gtcggcgagc acgctgtcgc gcggttcgcg ttccgggatc gcaagcacac
     6241 gcgcgaatgc gcgcagcgcg gcgacgagcg gtccggcagt gtcgcgcgcc ggatccttgg
     6301 cggcgcgcgc gagctcgtgc tcgaccttct tgcgcgccgc tttcacttcg gcagcttctt
     6361 cgcgttgcgt ctgggtgcgg agctgccagc tgcgcacgcc gaacagcgcg agcggaatcg
     6421 cgtagagccc ggcgatgagt agccagagca gcgcgccgcc gagcggcctg ctgtcgacga
     6481 caccgggcgc cgacagcgcg aggtcggcac cgacgagcgc gagatccgct tcgggcaggt
     6541 tggcggcgtg gctgccgtgc ttcggggaca tcgcgacgac gtcaccggcg ccgacgatgc
     6601 tgccaccctt caccgacacc gcgatcggat cgctgtggat cgtctgatag tgacccttga
     6661 ctggatcgaa gtacgagaac gcgatcgcgg gaatctccgt cgccggtccc gtcacctgcg
     6721 cggtgacctt gaacgtcttg ctcagaccgt cgtcggcgag ctcgccggtc ggcgcttccg
     6781 ccgggaccgt gaacttgtcc ttcggcagac cgcccggcgc gtcgaggtgg ccgagcgaga
     6841 gtgtgtcgag tcgctggttg ctcttcaccg tgatcgtgag ctcgaccggt tcgccgagct
     6901 ggacgaccga gcggctcgtc gcgactgcga tcgagaactg agagccgacc gcaccggcaa
     6961 atcccggcgg cttgtcggtc tccggcagcg gcttgacctc gagcgtcttg gcggcgccgc
     7021 tcgtccggaa catccgcgtc ggtgcgttgc cgaagaagtc gcggcggccg accgcgagtg
     7081 cggcgacgac cgtcgggcct ggcacttcga gcttgccggg cttcttcggc gcgacgtaaa
     7141 agcgcacggt caccttcttg aacgcaccgt cgtcggcgac gtcgtagggc agcttcagct
     7201 ctttgctgcc tgccggcaac gaaagtgcgc ggcgcggatc ggtcgccggc ggcgcgctga
     7261 tgatgaattc gtccatcgac atcagcggca ccgagaacgt ctgactctcg ggctcggtcc
     7321 ggaagcgcca gatcagcgtc gcttcgatcg tctcgccgac gaacaccgtg cggtcgggca
     7381 gggcgagctc gatcttcatg tcgtcggtgg tcggcaccgc ttcggcgtcg aggtcgccgg
     7441 cctgtgtggt cacgcgcttg ctgccttgca ccaccgtcac cgccggtacc cgtacgcggc
     7501 ccgctttgtc gagctcgaca cgccagcgca gcgtccacgt cactcgtttg aagtcgctgc
     7561 gcttgccgtt gatgatctgg atcgactgcg acaccgacgg ctcgccgccg agcggcgtga
     7621 cgcgcgcacc aggaatgtcg agcttgggct gctgcggctg cggctgctcg tcgaagccgg
     7681 caatcaccat cgcgatgttg aacggcacgc cggcgtgcgg ctgactttcg agccgcagct
     7741 ccgcgcgcgg ttcttcagcc gacgcaatgc cggcgagtcc gagcagcacc aggatgccga
     7801 tcgcgagccg cttcattacc agtccttctc cacggtgtcg ccgccggatt cttgctcttt
     7861 gcgctcgcgc tgtcgacggg cgtcttcatc gcgtgcgcgc tggccggcgc caccctgctg
     7921 cggctgctgt tcctgcttct tgtccttgtc ctgctgttgc tgctgttgct gcttgtcctt
     7981 gtcctgctgc tgctgttgcg gcggctggag cagcttgagc gcgttcatca ggtgctcgat
     8041 cgcagaggcc tggctcttga tcgtcggctc gaggctgaac gactggttcg tggtctgcac
     8101 tgccctgtcg agcgtgcgct tggcatcggc catgtcgttc tgtgcgaacc gcacttcgtc
     8161 ggcagccgcc gccatgttct tgccttgttg ctgctgttgc ggatcctgcg ctttcgccgc
     8221 ggcatcggcc tgctgtgcga gcgcgtcggt gatcgccttg gcgagctgcc cgtgctggtc
     8281 ctggcggctc gcgatgccgg gtagcttcgg cgcgcgcgtt tcatcgtcct cggtgtgtgc
     8341 cgt
//
LOCUS       DASUFX010000462        12711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1879879, whole genome shotgun sequence.
ACCESSION   DASUFX010000462 DASUFX010000000
VERSION     DASUFX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12711
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879879"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFV99_18515"
     CDS             <1..578
                     /locus_tag="VFV99_18515"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5061371.1"
                     /translation="LLAQDYRPARTIQFVAYAAEEVGLVGSTQLAKAYKQRGIDVVGA
                     LQLDMTNYQGSDKDIWLMKDFTSAAQNTFITRLIDTYVGATWGLDACGYACSDHASWY
                     RAGVPASMPFESRMRDRNKSIHTADDTLELSNNNADHALKFAKLGAAYAVEMAKGELG
                     PPPADEHGHRWAWLAGLGALVIAAGALTRRT"
     gene            652..987
                     /locus_tag="VFV99_18520"
     CDS             652..987
                     /locus_tag="VFV99_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061372.1"
                     /translation="MRRPREARRLQFVDGDMETEITCRIPRETLVGLLDTMDPIENQR
                     ITAEIPVAVPLEPDDSERITAEIQAVAMPAELVIRFNEATSLNHARMVVILASFVVAL
                     WVGLLTLVL"
     gene            complement(988..3543)
                     /locus_tag="VFV99_18525"
     CDS             complement(988..3543)
                     /locus_tag="VFV99_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX5061373.1"
                     /translation="MDRTLIVSATNVLARGYYAVPIDRKAPNGEPTNALFAVARALDK
                     ALAFKLPTRAVAVVESQPALDAWPEILRQQLPRLVPLLQALGLPVVEAPGELHLVASY
                     ARAAIDAGDDVIVVGTDKRYAQLVDERVWWYDPNKDARYTIEMVTKRFGVPPPQVGEW
                     LALVGNPDDALPGIAGIGAKGATDLLVKYGSVAAALAALDSIKGRAGNALRAAGDAID
                     VELTRTRLERDRPLPHPLAELTFALPEAGPKNAMFRELGFVEYLASDGHDDRLDVSVC
                     ESAADVQAAIAALGTAPVAMHPLVEDPTPVLGALVGIVLTAGGQRAWYVPAAQLAALR
                     PWLENAAASKLGHDTKVLDAALRKLGIQLAGVVGDTACASHLTEPSNWATHDLPIVAK
                     YAVGRALPEEDRVRGVGQKRKAWGELDVYVAGRYAAQLADAVVEVWGKLEPTVDKTLH
                     AEYLALSDTLVRMELAGIGVDAAELDRAAEAFAPIEAGLEREIYEIAGHELNLNSSKQ
                     LGALLFEQLKLPIVSHTKTGWSTATEALERIEHAHPVVRLVIRWRTLRRLRDSWINSL
                     RGYIAPDGRVHAWFHPARSFSGRIISTAPDLSRVPGRTPEMARIRRAFVPRPGNCLMS
                     VDYNQLGLHVLAHLTKDPALVEPLRRGEDVHVQTAAAVLELPVEAIGEAQRQIGKVVN
                     FATFAGQGSSALALQLNMSAQEAKQLIARFDRKYSLVREFQDEQHRLARERGYIVTIA
                     GRHWPIGGLESLDPHDRSYAERLSRRATHEGSVSDVSRRGLLDADRALRAAGLSAVPL
                     VQILDEVLFEVPEAEVRDAARIAADAMRNAFALEAPLKVGVEVGPTWADLQPR"
     gene            3603..4811
                     /locus_tag="VFV99_18530"
     CDS             3603..4811
                     /locus_tag="VFV99_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5061374.1"
                     /translation="MTVRWLVLLTVLAGCGNGKPPDIIGLKDQVAVVGQQLVLEIDGT
                     DPDGDNLSYSVDADISLEGNATMTQTPSGQGLFRWTPLASDVGAHGFDFTASDGSNDT
                     TVTITIDVRAASGGVPIFRQPLGAGRVVNLATDPCVNLDIVVEDQDTAQVTIAEEEPL
                     IAGATFNQLDGTTAQWQWCPTPAQVAQSDRYTLVLSADDGDNPKTIKMYVLVLGGGGG
                     GPSIVINEVDYDNVGTDSSEYLELFNPSGGSTSLAGLAVVLVNGATNTDYATIDLSAS
                     GSLPAGKYLVIAGASVTVPASAMKIDPVWTTDEIQNGGPDGIALVDTVKQVVIDAISY
                     EGSITAASLSGFTQPITLVEGQALDPSVADSTTVTKTLCRSPNGQDTNDANTDWKLCN
                     TRTVGTANVP"
     gene            complement(4808..5374)
                     /locus_tag="VFV99_18535"
     CDS             complement(4808..5374)
                     /locus_tag="VFV99_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061375.1"
                     /translation="MRSVLIIVLATGVASADDPPDSTEPTELHEGLTREATGPLLHIE
                     TALRPAAEGFDADVRREQVDLDLGPRTYAQFESSAYARFEDGMPKGWTTSLFNSEGWS
                     TTLRMVHDFKVIQVGAEASYNRVDSAYAHGSYAFVGASLTRMRKLSRWMTGWISLSLG
                     YTYWLGGQPPPGERNGAAGMLTIGTTFR"
     gene            complement(5409..6464)
                     /locus_tag="VFV99_18540"
     CDS             complement(5409..6464)
                     /locus_tag="VFV99_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061376.1"
                     /translation="MRCVVAVVLVVGCYSPSPHSGSPCVDGTCPTPLVCSQATLTCEY
                     SDMPIDADVGMADSPPDSPPDAQLIDGCTPAAELCGDGIDQDCDGNDPACAANDTAAG
                     AIDITAGGMFTGNLVFAHDDVAANGCSGNGGRDLYYSINLAAPRVYYFDTFGSNFDTV
                     IRVYGRSCATVGTGGMARACQNDSCNGTQSQVAVSLPAGPSCVVIDQASSAQTNGNVT
                     LKVIVGTRDGTALPAGMQMVSGDTTNATNVFDPVDVNCDGPGSGGRDLAYFFTACPNQ
                     SLQLDAETCTGASWDTVLYVRNGNTSQVGCNDDACGNNQSRITNVSITNGLFYWLVID
                     GYDNADYGTYTLKTYLR"
     gene            6578..8959
                     /gene="lon"
                     /locus_tag="VFV99_18545"
     CDS             6578..8959
                     /gene="lon"
                     /locus_tag="VFV99_18545"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5061377.1"
                     /translation="MSRNLPVIPMRSTVLFPGITLPISAGRPQTLRAIEAALRDPEHR
                     VLAVAQRSDSEEVKIEDLYSIGTVAKVSSVQRGLGGVRLLLEGVERVVIRHVTPQEGY
                     LVATFTEAVDLQPVDPKDNTFVALHREVRERAAELATKRGVPGEAVEQMLQQITEPGR
                     LADVVAGYLDVSTAERQTLLEMLAVEDRLRGVLIHVQRQLAILSAQEEIQSKVKEEFG
                     ERQREAILREQLKAIQKELGEGDEAADEALKELKAKLDKLPLPEEARKEVDREWARLT
                     RVGRESMESQVVRTYLETIAELPWGTRTEETLDVQQAAKILDEDHYGLKDVKDRVLEF
                     LAVYQLSKKKDNKGRILLFSGPPGVGKTSIAKSIARAMGRKYVRISLGGARDEADIRG
                     HRRTYIGALPGRIINGMKQAGSKNPVFLLDEVDKLGVSYQGDPASALLEVLDPAQNDS
                     FTDHYLGVPFDLSEVLFIATANFLQNIPGPLLDRMEVVDFSGYTEREKLEIAKHYLVA
                     RALADNGLTAEQLTLTDDAIREVITSYTREAGVRQLERELGRLARKLARRIAAKEIER
                     ATIDRADVRAALGRPRVHPEKANREDQVGIATGMYYTPAGGDIMFVEAALMRGKGDLI
                     LTGQLGDVMKESARAAWTFARAHADWLSIDDRVFERDVHVHVPAGAIPKDGPSAGIAM
                     ATAMVSALSGRPARHDVAMTGEITLSGRVLPIGGLKEKILGAVRAGITEFVIPKDNEP
                     DLEDLPAEVRDTIHVHPVELLAEALAITLRDTTLRDGRLYFGEPLSPLSELAH"
     gene            complement(8964..10652)
                     /locus_tag="VFV99_18550"
     CDS             complement(8964..10652)
                     /locus_tag="VFV99_18550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX5061378.1"
                     /translation="MYDPESLSKGQKVATMVGALLGLFLAALDQTIVSTAGPFIQKDL
                     GIEPGIYVWITTAYLVASTVLVPIYGKLSDLYGRRRILLIAIGIFLGGSLACGLSQSA
                     AQLIAFRAVQGAGSAGLFTSAFAIVADLFPPAERGKWQGIFGSAWGVSSVLGPLLGGV
                     LTENLSWHWTFFVNVPIGAAAVSLILSKMPPLRRTHDVKPKLDIAGALALALATVPLL
                     LALSLGHAEGTPAANGWPWQSWQIGALFGTSVAGIIAFLAIEKRAASPILDFKLFRIK
                     TFAIGSLATFVVGSAFLGAIVFLPLFMVNVVGLSATSSGFTTMPLTFGIVFANVTSGQ
                     LVSRIGAYKGLLVGSLVVLVSSFTLFALTLTTHETQGAVSLKMFLLGIGLGPSIPLFT
                     LAIQTSVQPQQIGVATAAATFFRQMGTTIGIAILGTIFATSFASSLRENMPRADQMPP
                     QMQAMMQRHGGGEGSARVAFDATKAKAAADKGIDMSPMPEAGKAAAKERAHAAIDGVD
                     HAFKQAFTSSLVSVFWVSLAIALLGLFITVFLPQLPLRRGPQGPGQQGPPPAVD"
     gene            complement(10652..11389)
                     /locus_tag="VFV99_18555"
     CDS             complement(10652..11389)
                     /locus_tag="VFV99_18555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5061379.1"
                     /translation="MMPTEIATQDGPCRAWVFRPEGTGPWPGVLFYMDGIGIRPALFE
                     MGERIAKHGYFVLLPDMFFRAGPYEAPDPKKLFGDPNVRQAWFEKIRPHMNPANAMVD
                     TRAFLDFLAAQADVRQPRFGTTGYCMGGGMSLTAAGTFPDRIAAAAAFHPAGLATDQP
                     TSPHLLAPKIKARVYVAGASEDQNFPDEQKARLDAALTDAGVAHTVETYPARHGWVPS
                     DTPVHDPAQAERAWTSLFELFDKTLRD"
     gene            11443..>12711
                     /locus_tag="VFV99_18560"
     CDS             11443..>12711
                     /locus_tag="VFV99_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061380.1"
                     /translation="MRAVTVVSVLLLAGVAVAAPPKYRKQPATSTAPAIVEAERLWNA
                     AASVTDRSLESDAWARAAEAFLAAADIATLSKTEQSEAAYSALLAIKNALNVDPRVKQ
                     TTDADTDWDRKPTPRQIPPREQRFIQIVRTYLKFDATSDEAVSVKFLYGNMLRRYDHF
                     DEAIPIFTEVLEHKDHETAEYAANLLLDTYNRLQRYDELVALAEKLRADTAFMAKHED
                     LAETVQKIHRQSVTLGARRCTEQARNDVRSAHERCGEMYLTAAADDKNHGDEALYNAL
                     VSFQDAGAIDRVREIDVMFDKRFPRSVLRTRAKMRRIVLEEAIGNYAVAVELSERLLA
                     RLDVNDKDAIGLTEKTVHLQILLGDLERAERSLAKLSARKPLQRESEARLTLQLIEAH
                     LAKGARADAQRLARNSDLRMNPTEQAFALEA"
BASE COUNT         2242 a         4185 c         4291 g         1993 t
ORIGIN      
        1 tgttgctcgc ccaggactac cgccccgcgc gcacgatcca gttcgtcgcg tacgcggcgg
       61 aggaggtcgg cctcgtcgga tcgacgcagc tcgcgaaggc gtacaagcag cgcggcatcg
      121 acgtcgttgg cgcgctccag ctcgacatga cgaactacca ggggtccgac aaggacatct
      181 ggctgatgaa ggacttcacg agcgcggcgc agaacacgtt catcacgcgg ctgatcgaca
      241 cgtacgtcgg cgcgacgtgg ggcctcgacg cgtgcggcta cgcctgttca gaccacgcga
      301 gctggtatcg cgcgggcgtc ccggcgtcga tgccgttcga atcgcgcatg cgcgatcgca
      361 acaagtcgat ccacaccgca gacgacacgc tcgagctgtc gaacaacaac gccgatcacg
      421 ccctcaagtt cgcgaagctc ggcgccgcct acgcggtcga gatggcgaag ggcgagctcg
      481 gcccgccacc cgccgacgag cacggacacc gctgggcatg gctcgccggg ctcggggcgc
      541 tcgtgattgc ggctggtgcg ctcacccgcc gtacgtagac cgcgcgggcg tggcgtgcga
      601 gcacgctgaa ccaaatgtct cactagcgcg cacccgacta cctccgcgtg gttgcggcgc
      661 ccacgcgagg cacggcggct gcaattcgtc gacggcgata tggagaccga gatcacttgt
      721 cgcatcccgc gcgagacgtt ggtcgggctg ctcgacacga tggatccgat cgagaaccag
      781 cggatcacgg ccgaaatccc ggtcgccgtg ccgctggagc ccgacgacag cgagcgaatc
      841 acggctgaga ttcaggcggt cgcgatgccc gcggagctcg tgattcgctt caacgaagcg
      901 acctcgctga atcacgcgcg catggtcgtg atccttgcaa gcttcgtcgt cgcgctctgg
      961 gtcggcctcc tcacgctcgt gctgtgatca cctcggttgc aggtcggccc acgtgggccc
     1021 gacctcgacg ccgaccttga gcggcgcttc gagcgcgaac gcgttacgca tcgcatcggc
     1081 ggcgatgcgc gcggcatcgc gaacctcggc ttcgggcacc tcgaacaaca cttcgtcgag
     1141 aatctggacg agcggcaccg ccgacagtcc cgcggcacgc agcgcgcgat cggcgtcgag
     1201 caagccacgg cgcgaaacat cggacaccga cccttcgtgc gtcgcgcgcc gcgagagccg
     1261 ctccgcatac gagcgatcgt gcggatcgag cgactcgagc ccgccgatcg gccaatgccg
     1321 ccccgcaatc gtgacgatgt agccgcgttc gcgtgcgagt cggtgctgct cgtcctggaa
     1381 ctcgcgcacc agcgagtact tgcgatcgaa gcgcgcgatc aactgcttcg cttcctgcgc
     1441 ggacatgttg agctgcagcg cgagtgcgct cgaaccttgc ccggcgaacg tcgcgaagtt
     1501 gacgaccttg ccgatctgcc gctgcgcctc accgatagcc tcgacgggca gctcgagcac
     1561 ggcagccgcc gtctgcacgt gcacgtcttc accgcgccgc aacggctcga cgagggcagg
     1621 gtccttcgtc aggtgcgcga gtacgtgcag gccgagctgg ttgtagtcga ccgacatcaa
     1681 acaattgccc ggccgcggca cgaacgcgcg ccgaatgcgc gccatctcgg gcgttcgccc
     1741 gggcacgcgg ctgaggtcgg gcgcggtgct gatgatgcgt cccgaaaacg atcgcgcggg
     1801 atgaaaccac gcatgcacgc ggccatccgg tgcgatgtaa ccacgcagcg agttgatcca
     1861 gctgtcgcgc agccggcgta gcgtgcgcca gcgaatcacg agccgcacga cgggatgcgc
     1921 gtgctcgatc cgttcgagcg cctcggtcgc cgtgctccag cccgtcttcg tgtggctgac
     1981 gatcggtagc ttcaattgct cgaacagcag cgcgccgagc tgcttcgacg agttcagatt
     2041 cagctcgtgc ccggcgatct cgtagatctc gcgctcgagc cccgcttcga tcggtgcgaa
     2101 cgcctccgcc gcccgatcga gctccgccgc atcgaccccg attccggcca gctccatccg
     2161 cacgagcgtg tccgagagcg cgaggtactc ggcgtgcaac gtcttgtcga ccgtgggctc
     2221 gagcttgccc cacacctcga cgaccgcgtc ggcaagctgc gccgcgtacc tgcccgcgac
     2281 atacacgtcg agctcgcccc acgccttgcg cttctggccg acaccgcgca cgcgatcttc
     2341 ctcgggcaac gcgcgcccga ccgcgtactt cgcgacgatt ggcagatcgt gggtcgccca
     2401 gttgctcggc tcggtcagat gcgaggcgca cgcggtatcg ccgacgacac ccgcgagctg
     2461 gatcccgagc ttgcgcagcg ccgcatcgag cactttcgtg tcgtggccga gcttcgatgc
     2521 cgcggcattc tcgagccacg gccgtagcgc cgcgagctga gctgccggca cgtaccacgc
     2581 ccgctggcca ccggcggtca acacgatgcc gaccaatgca ccgagcaccg gcgtcggatc
     2641 ctcgacgagc ggatgcatcg caaccggtgc cgtgccgagc gccgcgatcg ccgcctgcac
     2701 atcggccgcg ctctcgcaca cgctgacgtc gaggcgatcg tcgtggccat ccgacgccag
     2761 gtactcgacg aagccgagct cgcggaacat cgcgttcttc ggtcccgctt ccggcaatgc
     2821 aaacgtcagc tccgcgagcg gatgcggcag tggccgatcg cgctcgaggc gtgtccgcgt
     2881 cagctcgacg tcgatcgcgt cgcctgcggc ccgcagcgcg ttgcccgcgc ggcccttgat
     2941 cgaatcgagt gcagcaagcg cagctgccac cgagccgtac ttcacgagca agtcggtcgc
     3001 gcccttcgcc ccgatgccgg caatgccggg caacgcatcg tcgggattgc cgaccagcgc
     3061 gagccactcg ccgacctgcg gtggcggcac gccgaaccgc ttcgtgacca tctcgatcgt
     3121 gtagcgggcg tccttgttcg ggtcgtacca ccacacgcgc tcgtcgacga gctgcgcata
     3181 gcgcttgtcg gtaccgacga cgatcacgtc gtcgcccgcg tcgatcgcgg cgcgtgcata
     3241 cgacgcgacg aggtgaagct cgccgggcgc ctcgaccacg ggcagcccga gcgcctgcag
     3301 gagcggcacg aggcgcggca actgttggcg caggatctcc ggccatgcgt cgagcgcagg
     3361 ctggctctcc accacggcga ccgcgcgggt cggcagcttg aacgcgagcg ccttgtcgag
     3421 cgcgcgggcg acagcgaaca gcgcgttcgt cggttcgccg tttggcgctt tccgatcgat
     3481 cggcacggcg tagtagccgc gcgccagcac gttcgtggcc gagacgatca gtgtgcgatc
     3541 catcgcaggt gagaggcgtt atcgcatgcg gcgccgccga ggaaacgacg tacaatgcgt
     3601 tcgtgactgt ccgctggtta gtgctcttga ctgtgctcgc tgggtgcggg aacggcaagc
     3661 cgccggacat catcggcctg aaagaccagg tcgcggttgt cggccagcag ctcgtactcg
     3721 agatcgatgg cacggatccg gacggcgaca acctctcgta cagcgtcgac gcagacatct
     3781 cgctcgaagg caacgcgacg atgacgcaga cgccgagcgg gcagggcctc ttccgctgga
     3841 cgccactcgc gtcggatgtc ggcgcgcatg gcttcgactt cacggccagc gacggctcga
     3901 acgacacgac ggtcacgatc acgatcgacg tgcgcgctgc atcgggcggc gtgccgatct
     3961 ttcgccaacc gctcggcgcg ggccgcgtcg tcaacctcgc aaccgacccg tgcgtgaacc
     4021 tcgacatcgt ggtcgaggac caggacaccg cacaggtgac gatcgcggag gaagagccgc
     4081 tgatcgccgg agctacgttc aatcagctcg acggcacgac cgcgcagtgg cagtggtgcc
     4141 cgacgccggc ccaggtcgca cagagcgatc gctacacgct cgtgctgtcg gcggatgacg
     4201 gcgacaaccc gaagacgatc aagatgtacg tgctcgtgct cggcggcggt ggtggcgggc
     4261 cgagcatcgt gatcaacgaa gtcgactacg acaacgtcgg caccgacagc tccgagtatc
     4321 tcgagctgtt caatccgtcg ggtgggtcga cctcgctcgc cggtctcgcg gtggtactcg
     4381 tcaacggcgc gacgaacacc gactacgcga cgatcgatct gtcggcgagc gggtcgctgc
     4441 cggccggcaa gtacctcgtg atcgcgggtg cgtcggtgac agtgccggcg tcggcgatga
     4501 agatcgatcc cgtgtggacg acagacgaga tccagaacgg cgggccggat ggcatcgcac
     4561 tcgtcgatac ggtgaaacag gtcgtgatcg acgcgatctc gtacgagggc tcgatcacgg
     4621 cggcgtcgct cagtggcttc acgcagccga tcacgctcgt cgaggggcag gcgctcgatc
     4681 catcggttgc cgactcgacg accgtgacga agacgctgtg ccggagtccg aacggtcagg
     4741 acacgaacga cgcaaacacc gactggaagc tgtgcaacac gcgcacggtc ggtaccgcaa
     4801 acgtgcccta gcgaaacgtg gtgccgatcg tgagcatgcc ggcagcgccg tttctctcgc
     4861 cgggcggcgg ctgaccgccg agccaatacg tgtagccgag tgacagcgaa atccagccgg
     4921 tcatccacct cgacagcttc cgcatacgcg tgagcgaggc gccgacgaac gcgtagctgc
     4981 cgtgcgcgta tgcgctgtcg acgcggttat acgacgcttc agcgccgacc tggatgacct
     5041 tgaagtcgtg gaccatgcgc agcgtcgtgc tccagccctc gctgttgaac agcgaggtcg
     5101 tccagccttt cggcataccg tcctcgaacc gtgcatacgc gctgctttcg aactgcgcgt
     5161 atgtacgcgg gccaagatcg agatcgacct gctcgcgtcg cacgtcggcg tcgaaccctt
     5221 cggcggcggg gcggagcgcg gtctcgatgt gcagcaacgg gccggtcgcc tcgcgggtca
     5281 ggccctcgtg cagctcggtc ggttcggtcg agtcgggcgg gtcgtcggcg gatgcgacac
     5341 cggtcgcgag cacgatgatg agcacggagc gcacacccgt gcgacctgcg aaccggcggg
     5401 agggttgctc agcgcagata ggtcttcaac gtgtaggtgc cgtagtccgc gttatcgtag
     5461 ccgtcgatca cgagccagta gaacaggccg ttcgtgatcg agacattcgt gatccggctc
     5521 tgattgttgc cgcacgcgtc gtcgttgcag cccacctgtg acgtattgcc gttgcgtacg
     5581 tagagcacgg tgtcccacga cgcgcccgtg caggtctcgg cgtcgagctg gagcgactga
     5641 ttcgggcatg cggtgaagaa gtaggcgagg tcgcgaccgc ccgagcccgg gccatcgcag
     5701 ttgacgtcga ccgggtcaaa gacgtttgtc gcgttggtgg tgtcgccgct gaccatttgc
     5761 atgccggcgg gcagggcagt gccgtcgcgc gtgccgacga tgaccttgag cgtgacgttg
     5821 ccgttcgtct gggcggacga tgcttgatcg atgacgacgc agcttgggcc ggcgggcagt
     5881 gagaccgcga cctggctctg cgtgccgttg cacgagtcgt tctggcacgc gcgcgccatg
     5941 ccgccggtgc cgaccgtcgc gcacgagcgt ccgtagacgc gaatcacggt gtcgaagttc
     6001 gagccgaacg tgtcgaagta gtagacacgc ggtgcggcga ggttgatcga gtagtagaga
     6061 tcgcggccgc cgttgccgct gcagccgttc gcggcgacgt cgtcatgcgc gaagacgagg
     6121 ttgcccgtga acatgccgcc cgcggtgatg tcgatcgcgc cggcagcggt gtcgttcgcg
     6181 gcgcatgccg gatcgttgcc gtcgcagtct tgatcgatgc cgtcgccgca cagctcggca
     6241 gcgggcgtgc agccatcgat cagctgcgcg tcgggagggc tgtcgggagg gctgtcagcc
     6301 attccgacgt ccgcatcgat cggcatgtcg gagtactcgc aggtcagcgt cgcctgcgaa
     6361 cacacgagcg gtgtcgggca ggtgccatcg acgcacgggc taccggaatg tggcgacggc
     6421 gagtagcagc caacgaccag cacgaccgca acaacgcacc gcacacggca atgatctcgc
     6481 cagtgccgga gacgttgcaa caacgatcct aattcctccg atgacaggtc taacgggcgg
     6541 gttgccccgg gcacttgtca tgcacacctc cattggcatg tctcgcaatc tgcctgtcat
     6601 cccgatgcgc tctacggtgc tgtttccagg gattaccctg cccatctcgg cgggccggcc
     6661 gcagacgcta cgtgccatcg aagcggcact ccgtgacccg gagcaccgtg tgctcgcagt
     6721 cgctcagcgc agtgattccg aggaggtgaa gatcgaggac ctgtactcga tcgggaccgt
     6781 cgcgaaggtg tcgtccgtgc agcggggcct gggcggcgtg cgcctgctgc tcgaaggcgt
     6841 cgagcgtgtc gtcatccgcc acgtgactcc gcaggagggc tacctggttg cgacgttcac
     6901 cgaggcggtc gacctgcaac cggtcgatcc caaggacaac acgttcgtcg ccctgcatcg
     6961 cgaggtccgc gagcgcgcgg ccgagctggc gaccaagcgc ggcgtgcccg gcgaagcggt
     7021 cgagcagatg ctgcaacaga tcacggaacc gggccggctc gccgacgtcg ttgccggcta
     7081 cctcgacgtg tcgaccgccg agcggcagac gctgctcgag atgctcgcgg tcgaggatcg
     7141 tctgcgcggc gtcttgatcc acgtccagcg ccagctcgcg atcctgtcgg cgcaggagga
     7201 gatccagtcg aaggtcaaag aggagtttgg cgagcgccag cgcgaggcga tcctgcgcga
     7261 gcagctcaaa gcgattcaga aggagctcgg cgaaggcgac gaagctgccg acgaggcact
     7321 gaaggagctc aaggcgaagc tcgacaagct gccgctgccc gaggaagcgc gcaaggaagt
     7381 cgatcgcgag tgggcgcgcc tgacacgcgt cggtcgcgag tcgatggagt cgcaggtggt
     7441 ccgcacctat ctcgagacga tcgcggagct gccgtggggc acgcgcaccg aggaaacact
     7501 cgacgtccaa caagccgcga agatcctcga cgaggatcac tacgggctca aggacgtgaa
     7561 ggatcgcgtc ctcgagttcc tcgcggtcta tcaactgtcg aagaagaagg acaacaaggg
     7621 ccgcatcctc ttgttctcgg gaccgcctgg cgtcggcaag acgtcgatcg cgaagtcgat
     7681 cgctcgcgcg atgggacgca agtacgttcg catctcgctc ggcggcgcac gcgacgaggc
     7741 ggacattcgc ggccaccggc gcacgtacat cggcgcgctg ccaggtcgca tcatcaacgg
     7801 catgaagcaa gcgggtagca agaacccggt gttcttgctc gacgaggtcg acaagctcgg
     7861 cgtctcgtac cagggcgatc cggcgagcgc gctgctcgag gtgctcgatc cggcgcagaa
     7921 cgactcgttc accgatcact acctcggtgt tccgtttgat ctgtcggagg tgctgttcat
     7981 cgcaacggcg aacttcctgc agaacattcc ggggccgcta ctcgaccgca tggaggtcgt
     8041 cgacttctcc ggctacaccg agcgcgaaaa gctcgagatc gcgaagcact acctggtcgc
     8101 gcgtgcgctc gcggacaacg ggctcaccgc cgaacagctc acgctcaccg acgacgcgat
     8161 ccgcgaagtc atcaccagct acacgcgtga agcaggcgtg cgccagctcg agcgcgagct
     8221 cggccgactc gcccgcaagc tcgcgcgccg gatcgctgcg aaggagatcg agcgtgcgac
     8281 gatcgatcgc gccgacgtgc gcgcggcact cggccgaccg cgcgtgcatc ccgagaaggc
     8341 gaaccgcgag gatcaggtcg gcatcgcgac cggcatgtat tacacgccgg ccggcggcga
     8401 catcatgttc gtcgaagcgg cgctgatgcg cggcaagggt gacctcatcc tcaccggtca
     8461 gctcggcgac gtgatgaaag agtcagcacg cgccgcgtgg acgttcgcgc gcgcacatgc
     8521 ggactggctg tcgatcgacg atcgcgtgtt cgagcgcgat gttcacgtcc acgtgccggc
     8581 gggcgcgatt ccaaaggatg gtccgtcggc gggtatcgcg atggcgaccg cgatggtgtc
     8641 ggcactctcc ggccggcccg cgcgtcacga cgtcgcgatg accggcgaga tcacgctgtc
     8701 gggacgcgtg ctgccgatcg gtgggctcaa ggagaagatc ctcggcgcgg tgcgcgcggg
     8761 catcaccgag ttcgtgatcc cgaaggacaa cgagcccgat ctcgaggacc tgccagcgga
     8821 ggtgcgcgac accatccacg tgcatccggt cgagctgctc gccgaggcgc tcgcgatcac
     8881 gctgcgcgac acgacgctgc gtgacggccg gctgtacttc ggcgagccgc tctcgccgct
     8941 gtccgagctg gcgcactgac gggctaatcg acggccggcg gcggtccctg ctgacccggt
     9001 ccctgcgggc cacgccgcag cggcaactgc ggcaggaaca cggtgatgaa caggccgaga
     9061 agcgcgatcg cgagcgagac ccagaacacg ctgacgagcg agctcgtgaa tgcctgcttg
     9121 aacgcgtgat cgacgccgtc gatggcggcg tgcgcgcgct ccttcgcggc cgctttgccc
     9181 gcttcgggca tcggcgacat gtcgatgcct ttgtccgctg ccgcctttgc cttcgtggca
     9241 tcgaatgcga cgcgagcact gccttcgccg ccaccgtgtc gctgcatcat cgcctgcatc
     9301 tgcggtggca tctgatcggc gcgcggcatg ttctcgcgca gcgacgatgc gaacgacgtc
     9361 gcgaagatgg tgccgaggat cgcgatgccg atcgttgttc ccatctgacg gaagaacgtc
     9421 gccgcggccg tcgcgacgcc gatctgctgc ggctgcacgg acgtctgaat cgcgagcgtg
     9481 aacagcggaa tcgacggccc gagcccgatg ccgagcagga acatcttcag cgacaccgcg
     9541 ccctgcgtct cgtgcgttgt cagcgtgagc gcgaacaacg tgaagctgga caccagaacc
     9601 acgagcgagc cgacgagcag gcccttgtag gcgccgatcc gcgacaccag ctggcccgag
     9661 gtgacgttcg cgaacacgat gccgaacgtg agcggcatcg tcgtgaagcc cgagctggtc
     9721 gccgagagac cgacgacgtt caccatgaac agcggcagga acacgatcgc gccgaggaac
     9781 gcactgccga cgacgaacgt ggcaagcgag ccgatcgcga acgtcttgat gcggaacagc
     9841 ttgaagtcga ggatcgggct cgcggcgcgc ttctcgatcg cgaggaacgc gatgatgccg
     9901 gcgaccgacg tgccgaacag cgcgccgatc tgccacgatt gccatggcca gccgttcgcc
     9961 gcaggcgtgc cttccgcatg accgagcgag agcgcgagca gtagcggcac cgtggcgagc
    10021 gcgagtgcga gtgcgccagc gatgtcgagc ttcggcttca cgtcgtgcgt acggcgcagc
    10081 ggcggcatct tcgacaggat caggctcacc gcggcagcgc cgatcggtac gttgacgaag
    10141 aacgtccagt gccagctcag gttctcggtg agtacaccgc cgagcagtgg gccgagcacc
    10201 gacgacacgc cccacgccga gccgaagatg ccctgccact tgccgcgctc ggcgggtggg
    10261 aacaagtcgg cgacgatcgc gaacgcgctc gtgaacaatc cggcggagcc tgcgccctgg
    10321 actgcgcgga acgcgatcag ttgcgcagcg ctctgcgaca agccgcacgc gagcgagccg
    10381 ccgaggaaga tgccgatcgc gatgagcagg atgcggcgac ggccgtagag gtccgacagc
    10441 ttgccgtaaa tcggcacgag caccgtcgac gcgacgaggt atgcggtcgt gatccagacg
    10501 tagatgccgg gctcgatccc gaggtccttc tggatgaacg gacccgcggt cgagacgatc
    10561 gtctgatcga gcgccgcgag gaataggccg agcagggcgc cgaccatcgt cgcgaccttc
    10621 tggcccttac tgagtgattc ggggtcgtac atcagtcgcg cagtgtctta tcgaatagct
    10681 cgaagaggga agtccacgcg cgctcggctt gcgcgggatc gtgcaccggc gtgtcgctcg
    10741 gcacccagcc gtggcgcgcc ggatacgtct cgacggtgtg cgcgacaccg gcgtcggtga
    10801 gcgccgcgtc gaggcgcgcc ttctgctcgt cagggaagtt ctggtcctcg ctcgcgcccg
    10861 cgacgtagac gcgcgccttg atcttcggcg cgagcaggtg cgggctcgtc ggttgatctg
    10921 tcgcgaggcc ggcagggtgg aatgccgccg ccgcggcgat acggtcgggg aacgtgccgg
    10981 ccgccgtcag cgacatgccg ccgcccatgc agtaaccggt cgtgccgaag cgcggctggc
    11041 gcacgtcggc ttgcgcggcg aggaagtcga ggaacgcgcg tgtgtcgacc atcgcgttcg
    11101 ctgggttcat gtgcgggcgg atcttttcga accatgcctg gcgcacgttc ggatcgccga
    11161 acagcttctt ggggtcgggc gcctcgtagg ggccggcgcg aaagaacatg tcgggcaaca
    11221 gcacgaagta gccgtgcttc gcgatccgct cgcccatctc gaacagggca gggcggatgc
    11281 cgatgccgtc catgtagaac agcacgccgg gccacggtcc cgttccctcg ggccggaaca
    11341 cccaggcgcg gcacggaccg tcttgcgtgg cgatttcggt gggcatcatg ggagcacggt
    11401 tgtaccgcgc aacgcatgcg ccggcgcgtt gtctacgtgc ctatgagagc cgtcaccgtg
    11461 gtcagtgtgt tgctgctggc gggggtggcc gtggcggcgc cgccgaaata tcggaagcag
    11521 cctgcgacgt cgacggcacc ggcgatcgtc gaggcagaac gcctgtggaa cgcggctgcg
    11581 agtgtgacgg acagatcgct cgagagtgac gcgtgggcgc gcgccgccga ggcgtttctc
    11641 gcggccgccg acatcgccac attgagcaag accgagcaat cggaggccgc gtactccgcg
    11701 ctgctcgcga tcaagaacgc gctgaatgtc gacccgcgcg tgaaacagac gacggacgct
    11761 gacacggact gggatcgcaa accgacaccg cggcagattc cgccgcggga gcagcgcttc
    11821 atccagatcg tgcgcacgta tctgaagttc gacgcgacat cggacgaagc ggtcagcgtg
    11881 aagtttctct acggaaacat gctgcgccgc tacgatcact tcgacgaggc gattccgatc
    11941 ttcaccgagg tgctcgagca caaggatcac gagaccgccg agtacgccgc gaacctcttg
    12001 ctcgacacct acaaccgact gcagcgctac gacgagctcg tcgcgctcgc ggagaagcta
    12061 cgcgccgaca ctgcgttcat ggcgaagcac gaggacctcg cggagacggt gcagaagatt
    12121 cacaggcaaa gcgtgacgct cggcgcgcgg cgctgcaccg agcaagcgcg caacgacgtg
    12181 cggtctgcgc acgagcgctg tggcgagatg tatctcacgg cggcagccga cgacaagaac
    12241 cacggcgacg aggcgctcta caacgcgctc gtcagcttcc aggacgcggg agcgatcgat
    12301 cgcgtgcgcg agatcgacgt gatgttcgac aagagattcc ctcgctcggt cctgcggacg
    12361 cgcgcaaaga tgcgccgcat cgttctcgaa gaagcgatcg ggaactatgc agtcgccgtc
    12421 gagctttccg aacgcttgct ggcgcgtctg gacgtgaacg acaaagacgc gatcgggctc
    12481 accgaaaaaa ccgttcacct gcagatcctg ctcggcgacc tcgagcgtgc cgagcgcagc
    12541 ctcgcgaagc tgtcggcgcg caagccgttg cagcgcgagt cagaagctcg actgacgttg
    12601 cagctcatcg aagcacacct cgcaaaggga gctcgcgcag acgcgcagcg cctcgcgcgc
    12661 aacagcgacc tccgcatgaa cccgaccgaa caggcgttcg cgctcgaggc c
//
LOCUS       DASUFX010000463         7952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1185805, whole genome shotgun sequence.
ACCESSION   DASUFX010000463 DASUFX010000000
VERSION     DASUFX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7952
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185805"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFV99_18565"
     CDS             <1..228
                     /locus_tag="VFV99_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061381.1"
                     /translation="GAPGWTIDDVSFSGIVGTPFPAQVADDGVCEPPGDGSDDPIMSG
                     GGGCCDAGPLRGSSLVLALSVLGLVLRRRRP"
     gene            complement(498..1163)
                     /locus_tag="VFV99_18570"
     CDS             complement(498..1163)
                     /locus_tag="VFV99_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061382.1"
                     /translation="MVPWRELGRADVGGSQLVLAQRGDEYAIRMRGAELMNSRQHASE
                     EMLAKLGCAGLGRVDGARVLVGGLGMGFTLRAALDVLPQSARVTIVELVPEVVAWNRE
                     LIGDLARRPLEDPRVTVELGNVAAAIAKQQEAWHAILLDVDNGPDAFTSPANAALYGL
                     KGLIACRRALVKGGALGVWSVEDDKQFTDRMRGAAFDVDKQRVPARPNSTVKHVIWIG
                     RRK"
     gene            complement(1166..2137)
                     /locus_tag="VFV99_18575"
     CDS             complement(1166..2137)
                     /locus_tag="VFV99_18575"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5061383.1"
                     /translation="MTKYVCLFAALAALSACKKKSNEKPAETKGSAEVQAGDAAAAPA
                     VDAAPAAWDGKTPLTIEGFQTPESVLYDADGDVYLVSNINGAPTDPDDNGYISKVSPD
                     GKIAEEKWIDGVKANVKLDAPKGSAISNGTLYVADITVVRQFDAKTGEQKPDIKIEGA
                     TFLNDVAAAPDGGVYVTDSGLDAKFGPTNTDAVWHIGKDGKAKPLIKNKDLGHPNGIT
                     AGKDGSVWVVTFGTGEIYQVDAKGKKAAGQKLPKGQLDGIVVLDSGDVLVSSWEGSSV
                     FRGKPGGEWKPAVENVEAPADIGWDSKRKRLLIPQFKANKIILQPLE"
     gene            complement(2190..2447)
                     /locus_tag="VFV99_18580"
     CDS             complement(2190..2447)
                     /locus_tag="VFV99_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX5061384.1"
                     /translation="MSDHPTDFAGSIPDAITSGIKERIPDATVEVSGGGGHWNIVVVS
                     PVFAGKSMLESQRLVLGSIKHLINGANPPVHAVDSLVTKTP"
     gene            2432..3925
                     /locus_tag="VFV99_18585"
     CDS             2432..3925
                     /locus_tag="VFV99_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061385.1"
                     /translation="MDGRSRWDINRPLMRSLLASLLVFVACADAGDALVDETEVSPEL
                     DAKTDTATELSVRAGATTLWVSKTIARRGDDFVLRGRTSRTLTDGRGFIFDDVYGDFA
                     QKSPRVFELTWPVSTARGLVDGVDQFIGMTFTHSSSRPDSLTARVVVRPRLVTFTGSP
                     KIYLTAELTPIVYGGTVVYRISGHTYGANTGVRAVIDNVDLSDVRRIDSEKFAIDLSP
                     DQAFALTAGGDIQIIADFTTGGVDKRATLGLALKKLGMTASDAGTVWPPLTCATTVKS
                     CLLALPNGALDLGSCGEAVKVNACAGQVGVFVDDVAFQATLQAADMRLATTAAHTDAA
                     ALVGSDTANEWLFGAKETVETKLQQLFGRWYLSQAARTTALAAALEAGLDTAYARPLQ
                     IVEPHVANVTNAAAMRQVVADAVLAEIDRMDFEHTEFQRTLESLTHEFRAQHVADIKR
                     FRDSVQPEPYPGQPTWDVYADRWLGLYTEVAIERATGVIKAVLVEID"
     gene            4083..6065
                     /locus_tag="VFV99_18590"
     CDS             4083..6065
                     /locus_tag="VFV99_18590"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX5061386.1"
                     /translation="MRRSIVIAIGFAALAGVGCKKGSDSSAKQEPAKTQEGTPAKQDP
                     KASPAAAATDIGVTAGGIQHDDKEGPAGVVTAATGTVEVRRVGETAFAAAKADTKLYA
                     GDTVRTADNSTATITLADESVIEVSEVSTVALASRDGTADPASAAAVLGGVARFTVTP
                     RAPAEGPFRVYASTGVILTRGTVYGVGVSASGAARVGVESGAVDVIGIADFAAPAISV
                     PKAQQVVIAADGKVGAAAAWPADDWGTWRDDSDADVKLDATFDAHTAALTDLNKQLID
                     GYAELQTNADAAATFEATAATSAEKNDPAAYTAALPDGAATIDASFLLGARLEALTWA
                     YASHAALATDLYVRHPAELEAKWTVVGPQIDAAVLWPKRYDVTATAYFEPLRAQYYVH
                     HPRGRAHAQLVGIAVPQFYAQVEPPEIDPVTVRGKLHGQVWLVPQMTYKVQARPVWVA
                     APAPSWNANVHAVVAPPRAKVAWYVRPPNLKAKVLVGTNVSGQWKSKLDVQPPQPRAQ
                     LAAMWTIPVGMKVKIAPPDLSAAATARAKVKLGADGQLIRDHRVDIAAPSANVKADIK
                     GKVAVPDVKGKVDAKIGVGVPDVKGKIGVNVRDHRDAAVGAGVKAGGDVKAKVGGAVK
                     AGADVKVKIAVPKPPPPPSIKVEGKVKASGGIKIGN"
     gene            6129..6896
                     /locus_tag="VFV99_18595"
     CDS             6129..6896
                     /locus_tag="VFV99_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5061387.1"
                     /translation="MDHAAKVAETFLAPCGSELPADDMRVIQRDGWYQTITPSTKSTQ
                     GNEVTFSRVAQKDAERVARATIDDYTALGLPFKWSVGPLTEPADFGDVLERLGFRYWQ
                     GRGMAIEPARWSSVPPPGVTVERVTQATFEDYYTAFVRGWATEAVEAPSWRTSMRRAL
                     ERRHRYYLARVDGEPAGSGGFAIKPRSAYLVGGNVFERFRGRGVYRALIDARVRDLAE
                     LGIPLATTIARDATSAPILERFGFETLFRSRVYKYEP"
     gene            6893..7561
                     /locus_tag="VFV99_18600"
     CDS             6893..7561
                     /locus_tag="VFV99_18600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061388.1"
                     /translation="MKRLLILLTACASTPPPAAPAPHADPHAHHAMHHRFENADQWTR
                     VFDDPARDAWQMPDKVVATLALAPGMVVADVGAGTGYFEKRLSAAVGDSGAVIAVDIE
                     PDMVRYLRDRAQREGTPNVEARLGAPDDPKLTAASVDRVLIVDTWHHIDDRVAYSKKL
                     ATALKPGGAVFVVDFTLETEKGPPKQHRLAPAQVIEELTQAGLKAAVADAGLPDQYIV
                     KASL"
     gene            complement(7545..>7952)
                     /locus_tag="VFV99_18605"
     CDS             complement(7545..>7952)
                     /locus_tag="VFV99_18605"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5061389.1"
                     /translation="GAALMMLAGVAWGIYSLRGRGVTRPLVATAGNFALSVPIAAMLV
                     AIAFATRAHASPLGIALAATSGGIASGLGYSLWYAAVPSLGATRAAVVQLSVPVITAL
                     AAVVILDEPLRTTVAIGGAAIVGGLALALRAKP"
BASE COUNT         1298 a         2606 c         2738 g         1310 t
ORIGIN      
        1 ggcgcgccgg gttggacgat cgacgacgtc tcattctcgg gcatcgtcgg cacgccgttc
       61 cccgcgcagg ttgccgacga cggcgtgtgc gagccgccgg gcgacggttc cgacgatccg
      121 atcatgagcg gcggcggtgg ctgctgcgat gcgggcccgc tgcgcggcag ttcgctcgta
      181 ctcgcgctat cggtgctcgg cctcgtgctg cgccggcgtc gcccctgaga tcaaccgcca
      241 agacgccgga gcgcgaagcg cgccgctgcg ttggcgttcc gagctgcgcg cgcccagaga
      301 tcaaccgcca agacgccggc agacgccaag cccagagatc agtcgggtcc ggaacggacg
      361 tcgcgcggag cccgcacggc gctgggttcg tgttcgaacc gccgcgtgtg cgagcagaac
      421 gcgatcaaga tcgtcttctg atcttggcgt ctgcggcagc ttggcggttt agatccgagc
      481 gcgtcggggg cacgcggtta cttacgacgg ccgatccaga tcacgtgctt gacggtgctg
      541 ttcggtcgcg cgggcacgcg ctgcttgtcg acgtcgaatg cggcgccgcg catgcggtcc
      601 gtgaactgct tgtcgtcctc gacggaccac acgccgagcg caccgccctt cacgagcgcg
      661 cgcctgcacg cgatcaggcc cttcagcccg tagagcgcgg cgttcgccgg cgacgtgaat
      721 gcatcgggac cgttgtcgac gtcgagcagg atcgcgtgcc atgcctcctg ctgcttcgcg
      781 atcgcggcgg cgacattgcc gagctcgacg gtgacgcgcg gatcctcgag tggacggcgc
      841 gcgagatcgc cgatgagctc gcggttccac gcgacaacct ccggcacgag ctcgacgatc
      901 gtcacgcgtg cgctctgcgg cagtacgtcg agcgcggctc gcaacgtgaa gcccatgccg
      961 aggccgccga cgagcacgcg cgcaccatcg acgcggccga gcccagcaca gccgagcttg
     1021 gcgagcatct cttccgacgc gtgttgccgg ctgttcatca gctcggcacc gcgcatgcgg
     1081 atcgcgtatt cgtcaccgcg ttgcgcgagg acgagctgtg agccgccgac gtccgcgcgt
     1141 cccagctcgc gccaaggaac cacgactact cgagcggctg gaggatgatt ttgttcgcct
     1201 tgaactgcgg aatcagcagg cgcttgcgct tgctgtccca gccgatgtcg gccggcgctt
     1261 cgacgttttc gacggccggc ttccactcgc cgcccggctt gccgcgaaac acgctcgagc
     1321 cttcccagct cgacacgagc acgtcgccgc tgtcgagcac cacgatgccg tcgagctggc
     1381 ccttcggcag cttctggccc gcggccttct tgcccttcgc gtcgacttga tagatctcgc
     1441 cggtgccgaa cgtgacgacc cagaccgagc cgtccttgcc ggcggtgatg ccgttcgggt
     1501 ggccgaggtc cttgttcttg atgagcggct tcgccttgcc gtccttgccg atgtgccaga
     1561 ccgcgtcggt gttggtgggg ccgaacttcg cgtcgaggcc actgtcggtg acgtagacgc
     1621 caccgtcagg tgcggcagcg acgtcattca agaacgtcgc gccctcgatc ttgatgtcgg
     1681 gcttttgttc gccggtcttc gcgtcgaact gccgcacgac cgtgatgtcg gcgacgtaga
     1741 gcgtgccgtt gctgatcgca gagcccttcg gcgcatcgag cttgacgttg gccttcacgc
     1801 cgtcgatcca cttctcttct gcgatcttgc catcgggcga gaccttcgag atgtagccgt
     1861 tgtcgtcggg gtcggtgggc gcgccgttga tgttcgagac gaggtagacg tcgccgtcag
     1921 cgtcgtagag caccgactcg ggcgtctgga agccttcgat cgtcagcggc gtcttgccgt
     1981 cccacgctgc cggcgcggcg tcgacggcgg gagcagcagc ggcatcgccg gcctgcacct
     2041 cggccgaacc cttcgtctcg gcaggcttct cgttcgactt cttcttgcac gccgacagcg
     2101 cggcgagcgc ggcgaacaga catacgtact tcgtcatggc ggcagactaa cgccagccgg
     2161 caacggttca acacagccgc tgccgtaaat cacggcgtct tcgtcacgag cgagtcgacg
     2221 gcgtgcacgg gcggatttgc accattgatc aggtgcttga tcgagccgag tacgaggcgc
     2281 tggctctcga gcatgctctt gccggcaaac accggcgaga ccacgacgat attccagtgg
     2341 cctccaccac cagaaacttc gacggtggcg tcgggaatcc gctcctttat gccgctggtg
     2401 atggcgtcgg ggatcgagcc tgcgaaatca gttggatggt cgctcacgct gggatattaa
     2461 ccgccccctt atgcgttcgc ttctcgctag cttgctggtt tttgtcgcat gcgccgatgc
     2521 cggcgatgcg ctggtcgacg agactgaagt ctctccggag ttggatgcca agaccgatac
     2581 cgcgaccgag ctctcggtgc gcgccggcgc tacgacgctg tgggtgagca agacgatcgc
     2641 gcggcgcggc gatgacttcg tgttgcgcgg gcgcacgagc cgcacgctga ccgatggccg
     2701 cggcttcatc ttcgatgatg tctacggtga cttcgcgcag aagtcgccgc gcgtgttcga
     2761 gctgacgtgg ccggtgtcga ccgcgcgcgg gctggtcgac ggcgtcgatc agttcattgg
     2821 catgacgttc acgcactcga gctcccgccc ggattcgctg accgcgcgcg tcgtcgtacg
     2881 gccgcgcctc gtcacgttca ccgggtcgcc gaagatctac ctgacggcgg agctgacgcc
     2941 gatcgtctat ggcggcacgg tcgtctatcg gatcagcggt cacacctacg gcgcgaacac
     3001 cggcgtgcgc gccgtgatcg acaacgtgga cctctcggac gtgcggcgca tcgatagcga
     3061 gaagttcgcg atcgacctgt cgccggacca agcgttcgcg ctcactgcgg gcggcgacat
     3121 ccagatcatc gcggacttca cgaccggcgg cgtcgacaag cgcgcgacgc tcgggctcgc
     3181 gctcaagaag ctcggcatga ccgcctctga cgccgggacc gtgtggccgc cgctgacgtg
     3241 tgcgaccacc gtgaagagct gcttgctcgc gctaccgaat ggcgcgctcg atctcggcag
     3301 ctgcggcgag gccgtgaagg tcaacgcctg cgcgggtcag gtcggcgtgt tcgtcgacga
     3361 cgtcgcgttc caggcgacgc tgcaagcggc cgacatgcgg ctcgccacga cggcggcgca
     3421 cacggatgcc gccgcgctcg tcggcagcga cacggcgaat gagtggctgt tcggtgcgaa
     3481 ggaaacggtc gagacgaagc tgcagcagct gttcggccgc tggtacttgt cgcaagcggc
     3541 gcgcacgacc gcgctcgctg cggcactcga ggccgggctc gacaccgcgt acgcgcgacc
     3601 gctgcagatc gtcgagccgc acgtggcgaa cgtgaccaac gccgcggcga tgcgtcaggt
     3661 cgttgcggat gcggtgctcg ccgagatcga tcggatggat ttcgagcaca ccgaatttca
     3721 gcgcacgctc gaaagcctca cgcacgaatt tcgtgcgcag cacgtcgccg acatcaagcg
     3781 cttccgcgac agcgtgcaac cggagccgta tccggggcag ccgacgtggg acgtctacgc
     3841 cgatcgttgg ctcggcctct acaccgaggt cgcgatcgag cgcgcgacag gtgtaatcaa
     3901 agcagtactt gttgaaatcg attgattacg aagcgtgccg ctgcggcaca ctcgacgaga
     3961 aaatcgttcc cacggtcacg aatcgttcca gtcactaacg atttgttagg cctacatcgc
     4021 gtgtcgcggg ttgcgatgcc aactcgctgc gcggagactc gtcgtttgcg aaaggagaac
     4081 gcatgcgtcg ttctattgtg attgcgattg gctttgcggc gcttgctggc gttggctgca
     4141 agaagggcag cgacagctcg gcaaagcagg agcccgcgaa gactcaagaa ggcacacctg
     4201 ccaagcagga cccgaaggct tcgcccgcag cagcggcaac ggacatcggc gtcacggctg
     4261 gtgggatcca gcatgacgac aaggaaggcc cagcaggcgt cgtgaccgcc gcgaccggta
     4321 cggtcgaagt gcggcgcgtc ggcgagacgg cctttgcggc agccaaggcc gacacgaagc
     4381 tgtacgccgg tgacaccgtg cgcacggccg acaactcgac cgcgacgatc acgctcgccg
     4441 acgagtcggt gatcgaggtc tccgaggtct cgacggtcgc gcttgcgagc cgtgatggca
     4501 cggcggatcc cgcatcggcg gctgcagtgc tcggtggcgt cgcgcggttc acggtaacgc
     4561 cacgcgcgcc ggccgaaggt ccgttccgcg tctacgcgtc gaccggcgtg atcctcacgc
     4621 gcggcaccgt gtacggcgtc ggcgtgtcgg cttcgggtgc agcgcgcgtc ggtgtcgaga
     4681 gcggcgccgt cgacgtgatc ggtatcgctg atttcgccgc cccggcgatc tcggtgccga
     4741 aggcgcagca ggtcgtgatc gcggccgacg gtaaggtcgg cgcggcagcg gcgtggcccg
     4801 ccgatgattg gggcacgtgg cgcgacgata gcgatgcgga cgtcaagctc gatgctacgt
     4861 tcgacgcgca caccgcggcg ttgaccgatc tcaacaagca gctcatcgac ggctacgcgg
     4921 agctgcagac gaacgcagac gcagcggcga cgttcgaagc gaccgctgcg acgagcgcgg
     4981 agaagaacga tccggccgcg tatacggcag cgctgcccga cggtgcggcg accatcgatg
     5041 cgtcgttcct gttgggcgct cgcctcgaag cgctgacgtg ggcgtacgcg tcgcacgcgg
     5101 cgctcgcgac cgatctctac gtgcgccatc cggcggagct cgaagcgaag tggaccgttg
     5161 tcggtccgca gatcgacgcc gcggtgctgt ggccgaagcg ctacgacgtc accgcgacgg
     5221 cgtacttcga gccgctgcgt gcgcagtact acgtgcacca tccgcggggc cgcgcgcatg
     5281 cgcagcttgt cggtatcgcg gtgccgcagt tctacgcgca ggtcgagccg ccggagatcg
     5341 atccggtgac cgtgcgtggc aagctccatg gccaggtctg gctcgtgccg cagatgacgt
     5401 acaaggtgca ggcgcgcccg gtgtgggtcg cagcgccagc gccgtcgtgg aacgcgaacg
     5461 tccatgcggt cgtcgctccg ccgcgcgcga aggtcgcgtg gtacgtgcgc ccgccgaacc
     5521 tcaaggcgaa ggtgctcgtc ggcacgaacg tcagcggtca atggaagagc aagctcgacg
     5581 tgcagccgcc gcagccgcgg gctcagctcg ctgcgatgtg gacgattccg gtcggcatga
     5641 aggtgaagat cgcgccgccg gatctgtcgg ctgccgcgac cgcgcgcgcg aaggtgaagc
     5701 tcggcgctga tggccagttg atccgcgatc accgcgtcga catcgctgcc ccgagcgcga
     5761 acgtcaaggc ggacatcaag ggcaaggtcg ccgtgcccga cgtcaagggg aaggtcgacg
     5821 ccaagatcgg cgtcggcgtg cccgacgtga agggcaagat cggcgtcaac gtgcgcgatc
     5881 accgcgacgc cgcggtcggc gccggcgtga aggccggtgg cgacgtcaag gcgaaggtcg
     5941 gtggtgcggt gaaggccggc gccgacgtca aggtgaagat cgccgtgccg aagccgcctc
     6001 cgccgccgtc gatcaaggtc gagggtaagg tgaaggcgag tggcggcatc aagatcggta
     6061 actagccgtt agtttgtgag tgatgcacgc ggaggatcgc gaggtcctcc gcgtgtatcg
     6121 ttttcggcgt ggatcacgcg gcaaaggttg ccgagacgtt tctcgcgccg tgcggctcgg
     6181 agctgcccgc tgacgacatg cgcgtgatcc agcgcgacgg ctggtaccag acgatcacac
     6241 catcgacgaa atcgacgcag ggcaacgagg tgacgttctc gcgcgtcgca cagaaggacg
     6301 cggagcgcgt cgcgcgcgcg acgatcgacg actacacggc actcggcctg ccattcaagt
     6361 ggagcgtcgg cccgctcacc gagccagccg acttcggtga cgtgctcgag cggcttggct
     6421 tcaggtactg gcaaggacgc ggcatggcga tcgagcccgc acgctggtca tccgtgccgc
     6481 cgcccggcgt caccgtcgag cgcgtcacgc aggcgacgtt cgaggactac tacacagcgt
     6541 tcgtgcgcgg ctgggcgacg gaagcagtcg aggcgccgag ctggcgtacc agtatgcggc
     6601 gcgcgctcga gcgtcggcat cgttactatc tcgcgcgcgt cgacggcgag ccggcgggta
     6661 gcggcggctt cgcgatcaag ccgcgctcgg cgtatctggt cggcggcaac gtgttcgagc
     6721 gcttcagggg tcgcggggtc taccgggcgt tgatcgatgc gcgcgtgcgc gacctcgcgg
     6781 agctcgggat tccgctggcg acgacgattg cacgcgatgc cacgtcggcg ccgatcctcg
     6841 agcgcttcgg cttcgagact ttgttccgct cacgcgtgta caaatacgaa ccgtgaaacg
     6901 cctgctcatt ctgctcaccg cctgcgcgag cactccgcca ccagcggcgc ctgctcccca
     6961 cgccgatccg cacgcgcatc atgcgatgca tcatcggttc gagaatgcgg atcaatggac
     7021 gcgcgtgttc gacgatccgg cacgcgacgc atggcagatg cccgacaagg ttgtcgcgac
     7081 gctcgcgctt gcacccggca tggtcgtcgc cgacgtcggg gccggcacgg gctacttcga
     7141 gaagcggctg tcggcggccg tcggcgattc cggtgcggtg atcgccgtcg acatcgagcc
     7201 cgacatggtc cgctatctcc gcgaccgtgc gcagcgcgaa ggcacgccga acgtcgaggc
     7261 gcggctcggt gcgcccgacg acccgaagct cactgcggcg tcggtcgatc gcgtgttgat
     7321 cgtcgacacc tggcatcaca tcgacgatcg cgtcgcgtac agcaagaagc tcgcgacggc
     7381 gctcaagccg ggtggcgccg tgttcgtcgt cgatttcacg ctcgagaccg aaaagggtcc
     7441 accgaagcaa cacaggctcg caccggcaca agtgatcgaa gagctcacgc aagccgggct
     7501 gaaggcggcg gtcgccgacg ccggccttcc cgatcagtac atcgtcaagg cttcgctctg
     7561 agcgccagtg cgaggccgcc gacgatcgct gcgccaccga tcgcgaccgt cgtgcgcagt
     7621 ggctcgtcga gaatcacgac cgctgcgagc gcggtgatca ccggcaccga cagctggacg
     7681 accgccgcgc gcgtcgcccc gagcgatggc acggccgcgt accacaagct gtagccgagg
     7741 cccgatgcga tgccgccaga tgtggcggcg agcgcgatgc cgagcggcga cgcgtgagcg
     7801 cgcgttgcaa acgcgatcgc gacgagcatg gcagcgatcg gcacgctgag cgcgaagttg
     7861 ccggccgtcg cgacgagtgg acgcgtgacg ccgcggccgc gcaacgagta gatgccccac
     7921 gcgacaccgg cgagcatcat gagagccgca cc
//
LOCUS       DASUFX010000464        11077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_353211,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000464 DASUFX010000000
VERSION     DASUFX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353211"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV99_18610"
     CDS             <1..189
                     /locus_tag="VFV99_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061390.1"
                     /translation="ARSSKLAARSSKLEARSSKVEGRSSKLEARSSKLEARSSKLEAR
                     SSKLEAALLDDSERKADV"
     gene            complement(236..2008)
                     /locus_tag="VFV99_18615"
     CDS             complement(236..2008)
                     /locus_tag="VFV99_18615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HEX5061391.1"
                     /translation="MTPGGPMGGGGMGPSQPSGEEKKEGVAEAAPKTPGLLPTTPALP
                     PPKGRRKRWKLLELDGYFRMRTDWFKNFNLGFVDNPDLGGAPFPRALGCERLNGPCSN
                     SLSGTNMRLRLEPTFNLDEGTSVHVQADIYDNLVLGSTPIGENLSGIYNDTVPPPVGA
                     FFTDQDPPQAGVNSDRDAIRIKRVWAEVAVPLGILKFGRQPNHWGMGIYANAGGYDPI
                     SGTYDYDADYEDNVDRVSFSAVIPGTSLRAMIASDWNISRLVSNQTNQNKGQEGHPFN
                     LDPDDDENAWVGVISKMDSPQEFRDAVDRGDTAFNYGVYFEYKTQSWDDDLRGFTLGG
                     VFDGATRYVRRGLKTYTPDVWAKLGVGPVLLEGEFLAQFGKIENLEDAGITGTQDIRK
                     FGGVGRLTWKGVEGKLRLGLEAGFATGDEFDNTPQGRTNIAYSNLIGDPATCNAAHTC
                     TLTQFIFDRNYKVDMILWRYLVGAVTNAAYVKPFLQYDLTKSIMFKVANITSMALKPV
                     ATPGNSTMYGTEFNGDLGYNGHRLFAGISYGVLFPFAAMSHPDTDPTNGVGFPFGGPN
                     DPRGDNTGSASTAHSIQMRLILAF"
     gene            2385..3266
                     /locus_tag="VFV99_18620"
     CDS             2385..3266
                     /locus_tag="VFV99_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061392.1"
                     /translation="MKPPSPPAEPEPITRTPGPHKVDPNARRVVLGEMCPQGAGGRPA
                     VAPLIMRGVGWNDTAAEVAATVERGGVPHFAVFGVDGKVAGTFESLGMVEVGIAQPVA
                     TGTYVGASPCTYGGGKVTDTRAEDPKCGPATKGCGIAVGEITGPDEPERKPDFATGGA
                     CVVGNELEVDIDGDGKIESFPITGALDGIRAPAAEWTALPTSTQAPCKPTFQVYGMKL
                     APEPSSNKTPSKGVIVVDKGDVTIDVLGVIDLDGDGRREVILAMKFPTVRSIVVYTAT
                     EMAQRLELAGEASGFAK"
     gene            3488..4810
                     /locus_tag="VFV99_18625"
     CDS             3488..4810
                     /locus_tag="VFV99_18625"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HEX5061393.1"
                     /translation="MRSLCLAVALGSLAGCTDAGAHLTFSAPQGPGQVTSFQVVLATP
                     DQVPSIAGQRTSASGLEEQSVSYYLQRTIAGGTHGKIDGVDGFAVRVEPDPSMVETQF
                     IPFVLMYEGDKIVAVATFHAADRVTPSPILVLRDEIDKYVLDVEPVAQVDDMVPAAVS
                     QVRTVECFREDQSTFTSGIVWRTAGGAELRLLFPDDGGLDATGRALDLDCDHHPVAVD
                     TSGRDCDDSRAWFHNDAQETCDGFDTNCDGLQSLVVACDGNGVGGGVCTDPTTNTGLA
                     LCDDRTGTESACESDPQCLCAANPQLCARCVLTTELGTSTTTLKPCQPGVGYMHLEMC
                     NDTARCSRVEVVKTGGGWKAEVAADGTNLAFASVATNVGGKVFLRVKRPEGPGVEIPG
                     QRGTSTGDVMLAVTSADGSTHLRAIDLQFDVDGGTCTGSGPYAMLCYP"
     gene            4852..5976
                     /gene="nagZ"
                     /locus_tag="VFV99_18630"
     CDS             4852..5976
                     /gene="nagZ"
                     /locus_tag="VFV99_18630"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5061394.1"
                     /translation="MRDDVGQLLWVGFQGAIAPAGLRARLEAGGAGATILFKRNLVIR
                     TVAGGNAVPQEVIDLDALVALTRDLHRSAPDGTPALIAVDQEGGLVQRVRAPATMWPP
                     MMAHDGLPAPEDERVAEQVGRAIGEELRALGFDIDFAPVLDIHTNPANPIIGERAFGR
                     DAQTAARRALAFARGLDAAGMLACGKHFPGHGDTDTDSHLELPKVDHDWARLEAIELA
                     PFKLAAQANLPMIMTAHVVFAALDAKRPATLSEQVVTGLLRGKLGYRGVIVSDDLDMR
                     AIAGTMGVDHAAVQAIRAGCDVLLLCCNEQYQAQAEEALVKEAERDSEFKRRVGESAA
                     RVRAMKKAHAANQQKHPALGRDVVGSYEHRRLADRLAGRA"
     gene            complement(5995..6510)
                     /locus_tag="VFV99_18635"
     CDS             complement(5995..6510)
                     /locus_tag="VFV99_18635"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5061395.1"
                     /translation="MQEPLEGLDATAEAETSMSEVEPAAKPDIATLRRALSDLEAAKA
                     RVERDAQRAADDLREQVIAELLPVLDNLERTIHAAERGGASMAMMQGVRQVRHQFESV
                     LAKHGVERIEAKHQRFDPTLHDAISLVPVSHPSAHDVVVDVIEPGYKFGDRLLRPAKV
                     VVGRLQNQRYH"
     gene            complement(6549..8678)
                     /locus_tag="VFV99_18640"
     CDS             complement(6549..8678)
                     /locus_tag="VFV99_18640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5061396.1"
                     /translation="MGFLAPLMLAGIAAIVVPIAIHLIGRRRARVVKFAALDFLLATK
                     RKTAKRLRLRERLLLLVRALVCLAIAIALAKPFTSCERKGPMVTRGPQAAVLVIDDSF
                     ASGYLVDDKPWLYKAAEESRRILRQLGPEAEVAIVRASEAAEHPSELTRDHLRLRDQL
                     IALEPSARPADTTRALSRAAQLLAASSHVHKTVFLLSLQQRTGLRTDEAPWGKDGPQL
                     VVVDLRPAKMTNFAVTALGFDPDPGVGSRGVRFDAEVGNFGDTAATVELQLSVGDRVV
                     ARGSLELGPGEKKVKTFNATLPPGVRATDASVSLAGDKLAIDDRRWVSATLRDEVRVL
                     LVDGDPRTVRHDDELFYLEAALRPGDREDSGASVRTITADDLAGIEPNRRAGGAPAIN
                     IDDFDVVVLANVPALPAERVAVLAQWLHRGGGILIAPGDKVDPAAYDRTMLPLMPQSL
                     RDPIDTTWGVQPDEQDSRALHLVKWEADHPIFAPFSKDHPEQIADAKFFKISLLGPTT
                     ATADRRVLARYTNGAAALVEASIGAGRTLLFTSTLDRDWNDLPKYGAGYLPLVQQIVR
                     HLARKHARGAQSDHLVGSSVVLATGDLRRLEVRGPEGNGAVFEGDRINGRSSVRFGKT
                     DRPGIYRVVGTDQTGATRDREELAFVVNVDPRGSDLTAAPAKALPESGTGGGSAPVDT
                     RRRVELWHALAALVLLLLLAEGVLVQR"
     gene            complement(9014..9934)
                     /locus_tag="VFV99_18645"
     CDS             complement(9014..9934)
                     /locus_tag="VFV99_18645"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5061397.1"
                     /translation="MLQQKIDPAQLARLGSLPIKARVIVEGALSGLHRASVHGSSVEF
                     SEHKEYSPGDELRHIDWRAYAKGDRYYVKQYEQESQLTVYLVLDASASMTFAGGGIAK
                     LEYAGLALAALAYLVIQQQDKVGLLACGDRKIETLVPPRARSTHLHDLLSVMDQLMTK
                     GGVGDESPAAALERIAELSRRRRSLIVLASDLFDTDDKTLRALATLRAQKHDVSVLHV
                     LDPHERAFPYEGLTEFHALETTNRMLVNPAAIRRDYLERMEQFLAKTRGTLTAAGVDY
                     HLTSTDRALEATLIDLLMARSRLAPGRRAS"
     gene            complement(9944..10963)
                     /locus_tag="VFV99_18650"
     CDS             complement(9944..10963)
                     /locus_tag="VFV99_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5061398.1"
                     /translation="MNPTVPSDPATSRSDLEAVAELADAHKQIVTEIEKRIIGQRRVV
                     DQLLVALFARGHTLFVGVPGLAKTLLISTLAETLNLTFQRIQFTPDLMPADITGTEVL
                     EEDHTTGRRVFRFLKGPVFANLVLADEINRTPPKTQAALLQSMQEFRVTAGGTTHDLP
                     LPFLVFATQNPIEQEGTYPLPEAQLDRFMFQVDVDYPSEAEEEEIVRQQTSDHRPELS
                     RVLSPQRILQLQELVRRVPAADHVVKYAVALARSTRPSAPNAPPFIRDAVSWGAGPRA
                     SQFLILAAKARAILDGRFAVAIEDVAALARPTLQHRLILNYRAEAEGQRAGELVDRLL
                     SVVTP"
BASE COUNT         1822 a         3572 c         3825 g         1858 t
ORIGIN      
        1 gctcgaagct cgaagctcgc agctcgaagc tcgaagctcg aagctcgaag ctcgaaggtc
       61 gaaggtcgaa gctcgaagct cgaagctcga agctcgaagc tcgaagctcg aagctcgaag
      121 ctcgaagctc gaagctcgaa gctcgaagcg gcccttcttg atgactccga acgaaaggcc
      181 gacgtctgaa gccccactga ggcccgaggc ccgaggccct aggcccgagg ccttcttaga
      241 aggcgaggat caggcgcatc tggatcgagt gcgccgtcga cgcgctgccg gtgttgtcgc
      301 cgcgcggatc gttcgggccg ccgaatggga atccgacgcc gttggtggga tcggtgtccg
      361 ggtgcgacat cgcggcgaac gggaacagca cgccgtacga gatgccagcg aacaggcgat
      421 ggccgttgta gccgagatcg ccgttaaact cggtgccgta catcgtcgag ttacccggcg
      481 tcgcgaccgg cttgagcgcc atcgacgtga tgttcgcgac cttgaacatg atcgacttgg
      541 tgaggtcgta ctgcaggaac ggtttgacgt acgcagcgtt cgtcaccgca ccgaccaggt
      601 agcgccacag gatcatgtcg accttgtagt tacggtcgaa gatgaactgc gtcagcgtgc
      661 acgtgtgtgc cgcgttgcag gtcgcgggat cgccgatcag gttggagtag gcgatgttcg
      721 tgcggccctg cggcgtattg tcgaactcgt cgccggtcgc gaacccagcc tcgaggccga
      781 gccgcagctt gccctcgacg cctttccacg tcaggcgacc gacgccgccg aacttgcgga
      841 tgtcttgcgt gccggtgatg cccgcatcct cgagattctc gatcttgccg aactgcgcga
      901 ggaactcacc ttcgagcaag accgggccga caccgagctt cgcccagaca tccggcgtat
      961 acgtcttgag gccgcgccgc acgtagcgcg tggcaccgtc gaagacgccg ccgagcgtga
     1021 agccgcgtag atcgtcgtcc cagctctgcg tcttgtactc gaagtacacg ccgtagttga
     1081 acgcggtgtc gccgcggtcg accgcatcgc ggaactcctg cggcgaatcc atcttcgaga
     1141 tcacgccgac ccatgcgttc tcgtcgtcgt cgggatcgag gttgaacggg tggccttcct
     1201 ggcccttgtt ctggttcgtc tggttcgaga ccaggcggct gatgttccag tccgacgcga
     1261 tcatcgcgcg cagcgacgtg cccggaatca ccgctgagaa gctgacgcga tcgacgttgt
     1321 cttcgtagtc agcgtcgtag tcatacgtgc cgctgatcgg atcgtagccg cctgcgtttg
     1381 cgtagatgcc catgccccag tggttcggct gacggccaaa cttcaggatg ccgagcggga
     1441 ccgcgacctc ggcccagacg cgcttgatgc ggatcgcgtc gcgatcgctg ttcacgccgg
     1501 cctgcggcgg atcttgatcg gtgaagaagg cgccgaccgg cggcggcacc gtgtcgttgt
     1561 agatgccgct caggttctcg ccaatcggcg tcgagccgag gacgaggttg tcgtagatgt
     1621 ccgcttgcac gtgcacggac gtgccttcgt cgaggttgaa cgtcggctcg aggcggagcc
     1681 gcatgttcgt gccggacagc gagttggaac acgggccgtt cagccgttca cacccgagcg
     1741 cgcgcgggaa tggtgcgccg cccagatcgg gattgtcgac gaacccgaga ttgaagttct
     1801 tgaaccagtc cgtccgcatg cggaagtagc cgtcgagctc gagcagcttc cagcgcttgc
     1861 ggcggccctt cggcggcggc aatgcgggcg tcgtcgggag cagtcccggc gtcttcggcg
     1921 cggcctcggc gacgccttcc tttttctctt caccggaggg ctgcgagggg cccataccgc
     1981 cgccgcccat gggaccgcca ggggtcattt gcgcctcggc aatctgggcg ccgagaagca
     2041 ggatggcagt gcctacgaaa acctttgagc tcaagtaaga catgggaaaa tcgagggttt
     2101 ttggggcatt gccgaaagtg ggcggactat aggcaccgca cagtggcggg gtcaacgtcc
     2161 gtggctggaa ttggccgggg ccgtgggctt ggcgttatgg ttctgcgatg ccgtacgacc
     2221 gtgatggcag cggccgcgtt gccccagttg ggtcggaaat gtggtcgacc ggggcttctc
     2281 gaatgcgttt gcgacggtca aagcacgaga tggtgctttg ttttgaccgt gcatcgcgct
     2341 ggttggggat ctgtacgctg gtcctcgtag cctgcggacc ttcggtgaag cccccttcac
     2401 cacccgctga gcccgaaccg attacccgta ccccgggccc gcacaaagtc gatccgaacg
     2461 cacgccgcgt cgtgctcggc gaaatgtgtc cacaaggagc gggcggccga cctgcggtcg
     2521 cgccactcat catgcgcggc gtcggctgga acgatacggc cgctgaagtc gcagcgaccg
     2581 tcgagcgcgg tggtgtgccg cacttcgcgg tattcggcgt cgacggaaag gtcgccggca
     2641 cgttcgagtc gctcggcatg gtcgaggtcg gcatcgcgca gccggtcgcg accggtacgt
     2701 acgtcggcgc ctcgccgtgc acctatggcg gcggcaaggt caccgatacg cgcgccgagg
     2761 atccgaaatg cggtccggcg acgaaaggct gcggcatcgc ggtcggcgag atcaccggtc
     2821 ccgacgaacc cgagcgcaaa ccggacttcg ctacgggcgg tgcgtgcgtg gtcgggaacg
     2881 agctcgaggt cgacatcgat ggcgacggca agatcgagtc gtttccgatc accggtgcac
     2941 tggacgggat ccgggcgccg gccgccgaat ggacggcgct gccgacgtcg acgcaggcac
     3001 cgtgcaagcc aacatttcag gtctacggca tgaagctcgc ccccgagccg agcagcaaca
     3061 agacaccgag caagggcgtc atcgtcgtgg acaagggcga cgtcacgatc gacgtgctcg
     3121 gcgtgatcga tctcgacggc gacggccgtc gcgaggtgat cctggcgatg aagtttccga
     3181 ccgtgcgctc gatcgttgtc tatacggcga ccgagatggc gcagcggctc gagctcgcag
     3241 gcgaggcgag cggcttcgcg aagtagcccg tccggctcgt gagcgtccgc cctggacaac
     3301 gtccgagcga cggacgacct gcgggctgga cgcgacatgg ccaccggagc gacgccaacc
     3361 gcgttggttg gcgggctcac aatcgggcga gtcccaccta catcgccgat cacgtcggtt
     3421 cacgaggatc gcgatcgcag gaggtggcgg cggctgctgg cacggccgtg gcagtagctc
     3481 ccgcgatatg cgctcgcttt gcctagcagt ggctcttggc tcgctcgcgg gatgcaccga
     3541 cgcgggcgct cacctgacgt tctcggcgcc gcaaggcccg ggacaggtca cgtcgtttca
     3601 ggtcgtgctc gcgacgcccg atcaggtgcc cagcattgcc ggccagcgca cgtcggcgag
     3661 cgggctcgag gagcagtcgg tctcgtacta cctgcagcgc acgatcgccg gcggcacgca
     3721 cggcaagatc gacggcgtcg atggcttcgc agtgcgcgtc gagcccgacc cgtcgatggt
     3781 cgagacgcag ttcattccgt tcgtgctgat gtacgaggga gacaagatcg tcgcggttgc
     3841 gacgtttcac gcggccgatc gcgtgacgcc gtcgccgatc ctcgtgctgc gcgacgagat
     3901 cgacaagtac gtgctcgacg tcgagccggt cgcgcaggtc gacgacatgg taccggccgc
     3961 cgtctcgcaa gtgcgcaccg tcgagtgctt ccgcgaagat caatcgacgt tcacgagcgg
     4021 catcgtctgg cgtacggctg gcggtgccga gctgcgcctg ctgttccccg acgacggcgg
     4081 cctcgacgct actggccgcg cactcgatct cgattgcgat caccatccgg tcgctgtcga
     4141 tacgagcggc cgcgactgcg atgactcgcg cgcatggttt cataacgacg cgcaggagac
     4201 gtgcgacggc ttcgacacga actgcgacgg cctgcaaagc ctcgtggtcg cgtgcgatgg
     4261 caacggcgtc ggcggtggcg tgtgcacgga tccgacgacg aacaccggcc tcgcgctgtg
     4321 cgacgatcga accgggacgg aaagcgcctg cgaaagcgat ccgcagtgtc tgtgcgccgc
     4381 aaacccgcag ctctgcgcgc gctgcgtgct gaccaccgag ctcggcacgt cgacgacgac
     4441 gctcaagccg tgtcagcctg gcgtcggtta catgcacctc gagatgtgca acgacaccgc
     4501 gcgctgctcg cgcgtcgagg tcgtgaagac cggcggtggc tggaaggcgg aggtcgcggc
     4561 cgacggcacg aacctcgcgt tcgcgagtgt cgcgaccaac gtcggcggca aggtgttctt
     4621 gcgcgtgaag cgtccggagg ggccgggcgt cgagatcccg ggtcaacgcg gtacgtcgac
     4681 cggcgacgtc atgctcgcgg tcacaagcgc cgacgggagc acgcatttgc gcgcgatcga
     4741 tctgcagttc gatgtcgacg gcggcacgtg caccgggtcg gggccgtacg cgatgctctg
     4801 ctacccgtag cggattgttg tcgcgggcgc tgggcctgct actgtcgcgg cgtgcgcgac
     4861 gacgtcggtc agctcctctg ggttggtttc caaggcgcga tagctcccgc cggtttgcgt
     4921 gcgcggctcg aggctggcgg cgcaggcgcg acgatcctgt tcaagcgcaa cctcgtcatt
     4981 cgcacggtcg cgggcggtaa tgcggtaccg caagaggtga tcgatctcga cgcgctcgtc
     5041 gcgctcaccc gcgacctgca tcgcagcgcg cccgacggca cgccagcgtt gatcgccgtg
     5101 gatcaagaag gtggtctcgt ccagcgcgtg cgcgctccgg cgacgatgtg gccgccgatg
     5161 atggcgcacg atgggttgcc ggcgccggaa gacgagcgcg tcgccgagca ggtggggcgc
     5221 gcgatcggcg aagagctgcg cgcactcggc ttcgacatcg atttcgcgcc ggtgctcgac
     5281 atccacacga acccagcgaa tccgatcatc ggcgagcgtg cgttcgggcg tgacgcgcag
     5341 actgcagcgc gccgcgctct cgcgttcgcg cgaggcctcg acgccgctgg catgctcgcg
     5401 tgcggcaagc acttcccggg gcacggcgat accgataccg attcgcacct cgaattgccg
     5461 aaggtcgatc acgactgggc ccggctcgag gcgatcgagc tcgcaccgtt caagctcgcc
     5521 gcgcaggcga acctgccgat gatcatgacc gcgcacgtcg tgttcgcggc gctcgatgcg
     5581 aagcggccgg cgacgctttc agagcaggtc gtcacgggcc tgttgcgcgg caagctcggc
     5641 tatcgcggtg tcatcgtcag cgacgatctc gatatgcgcg cgatcgccgg caccatgggc
     5701 gtcgatcacg cggccgtgca ggcgatccgc gccggctgcg acgtgctgct cctgtgctgc
     5761 aacgagcagt accaggcgca agccgaggaa gcgctggtca aagaagcgga gcgcgatagc
     5821 gagttcaagc gacgcgtcgg tgaatcggcg gcacgcgtgc gtgcgatgaa gaaggcgcac
     5881 gcggcgaatc agcagaagca tcccgcgctc ggtcgcgacg tcgtcggctc gtacgagcac
     5941 cgccggttgg ccgaccggct cgccggccgc gcatgaacgc gcgagtgacg cgcgtcagtg
     6001 gtagcgctgg ttctgcagtc tgccgacgac gaccttcgcc ggacgcagca atcgatcgcc
     6061 gaacttgtag cccggctcga tcacgtcgac gacgacatcg tgcgccgacg gatgactcac
     6121 cggcaccagg ctgatcgcat cgtgcaacgt cggatcgaag cgctggtgct tcgcctcgat
     6181 gcgttcgacg ccatgcttgg cgagcacgct ctcgaattga tgccgcacct gacggacgcc
     6241 ctgcatcatc gccatgctcg cgccgccgcg ttcggctgca tgaatcgtgc gttcgaggtt
     6301 gtcgagcacg ggcaacagct cggcgatcac ctgttcgcgt agatcgtccg ctgcgcgctg
     6361 ggcatcgcgc tcgacgcgcg cctttgccgc ttcgagatcg ctgagcgctc gccgcaacgt
     6421 ggcgatatca ggcttggccg caggctcgac ctcgctcatc gaggtctcgg cttcagcggt
     6481 tgcgtccagg ccttccagag gttcttgcat gctgcacctt cgcagctacg agaatccaac
     6541 ttctgtgcct aacgctggac cagtacgcct tcggcgagga gcagcagcaa aaccagcgct
     6601 gcgagcgcgt gccacagctc gacgcggcgg cgcgtatcta ccggcgcgga accgccgccc
     6661 gtgccggatt ccggaagcgc tttcgcgggt gcggcagtga ggtccgatcc acgcgggtcg
     6721 acgttgacca cgaacgcgag ctcttcgcga tcgcgcgtcg cgccggtctg atcggtgccg
     6781 acgacgcgat agataccggg acgatcggtc ttgccgaagc gcaccgagct acggccgttg
     6841 atgcgatcgc cttcgaacac cgcgccattg ccctccgggc cgcgcacttc gagacgccgc
     6901 agatcgccgg tcgcgagcac caccgagctg ccgacgaggt gatcgctctg cgcaccgcgt
     6961 gcgtgcttgc gggcaaggtg gcgcacgatt tgctggacga gcggcaggta gccggcgccg
     7021 tacttcggca gatcattcca gtcgcgatcg agcgtcgacg tgaacagcag agtgcggccg
     7081 gcaccgatcg acgcctcgac gagggcggcg gcgccgttcg tgtagcgcgc gagcacccgg
     7141 cggtcagcgg tcgccgtcgt cggaccgagt agcgagatct tgaagaattt cgcgtccgcg
     7201 atctgctccg ggtgatcctt cgagaacggc gcgaagatcg ggtggtccgc ctcccacttc
     7261 acgagatgga gcgcgcggct gtcctgctcg tcgggctgga cgccccacgt cgtgtcgatc
     7321 ggatcgcgca ggctctgcgg catcagcggc agcatcgtgc ggtcgtacgc cgcaggatcg
     7381 accttgtcgc cgggcgcgat caggatgcca ccgccgcgat gcagccactg cgcgagtacg
     7441 gcaacgcgct cggcgggcaa tgcaggaacg ttcgccagca cgacgacgtc gaagtcgtcg
     7501 atgttgatcg cgggtgcgcc gccggcacgc cggttgggct cgatgcccgc gagatcgtcg
     7561 gccgtgatcg ttcgcaccga ggcgccgctg tcttcgcgat cgccgggccg gagcgcagcc
     7621 tccaggtaga acagctcgtc atcgtggcgg accgtacgcg gatcgccgtc gacgagcagc
     7681 acgcgcacct catcgcgtag cgtcgcactg acccagcggc ggtcatcgat cgcgagcttg
     7741 tcgcccgcga gcgataccga ggcgtcggtc gcccgcacgc caggtggcaa cgtcgcgttg
     7801 aacgtcttga cctttttttc gccgggcccg agctcgagcg agccgcgcgc gacaacccga
     7861 tcgccgaccg agagctggag ctcgacggtg gcggcggtgt cgccgaagtt gccgacctcg
     7921 gcatcgaagc gcacgccgcg gctgccgacg ccgggatccg ggtcgaagcc gagcgcggtg
     7981 accgcgaagt tcgtcatctt cgccgggcgc aaatcgacga cgacgagctg cgggccgtct
     8041 ttgccccacg gcgcctcgtc ggtgcgcaac ccggtgcgct gctgcagcga cagcaggaac
     8101 accgtcttgt gcacgtggct cgacgcggcg aggagctgcg cggcgcgcga caacgcacgc
     8161 gtggtgtcgg ctgggcgggc gctcggctcg agtgcgatca attgatcgcg cagacgcaag
     8221 tggtcgcgcg tcagctccga cggatgctcg gcggcctccg aggcgcgcac gatggcgacc
     8281 tcggcttcgg ggccgagctg acgcaggatg cggcgcgatt cttcggcggc cttgtacagc
     8341 cacggcttgt cgtcgacgag gtagccgctc gcaaacgagt cgtcgatcac gagcacggct
     8401 gcctgcggcc cgcgggtgac catcgggcct ttgcgctcgc acgacgtgaa tggtttcgcg
     8461 agtgcgatcg cgatcgcgag gcagacgagc gcgcgcacga gcaacagcag tcgctcgcgc
     8521 agccgcagcc gcttggcagt cttgcgtttc gtcgcgagca agaagtccag cgccgcgaac
     8581 ttcacgacgc gcgcgcgccg gcgcccgatc aggtggatcg cgatcggcac tacgatcgca
     8641 gcgatgccgg cgagcatgag tggcgcgagg aagcccacta cgaggggttc ctttcgtgcc
     8701 gacgtacacg tgcacgtgtc acgtgctcgt gcacgtacac gatcggctgc gccgcataaa
     8761 tcgctccaag agacatcgtc gggtccacga acgttgcgct cgggctgccg ccaaagccga
     8821 aacgctgaaa cgttgcgctc gggctgccgc caaagccgaa acgctgaggg ttgcgttcgt
     8881 agtcagcgga aggacgatcg agactcgaat cgcgcagttg ccggggcggc gcgagcactt
     8941 cctctgctcg tgtacgtgta cgtgcacgtg acacgtgaca cgtgcacgtg gagagggagg
     9001 cgagcgagcg catttacgac gccctccggc ccggcgccag ccgcgagcgc gccatcaaca
     9061 gatcgatcag cgttgcttcg agcgcgcgat cggtcgacgt caggtgatag tcgacgccgg
     9121 cggcggtcag cgtgccgcgc gtcttcgcga ggaactgctc catgcgctcg aggtaatcgc
     9181 ggcggatcgc ggcgggattg acgagcatcc gattcgtcgt ctcgagcgcg tggaactcgg
     9241 tgaggccttc gtacggaaac gcccgttcgt gcggatcgag cacgtggagc acactgacgt
     9301 cgtgcttctg cgcccgcagc gtcgcgagcg cgcgcagcgt cttgtcgtcg gtgtcgaaca
     9361 gatccgacgc gagcacgatc aggctgcgac gtcgccgcga tagctcggcg atgcgttcga
     9421 gcgcagccgc gggcgactcg tcgccgacgc cgcccttcgt catcagctgg tccatgaccg
     9481 acagcaagtc atgcaggtgc gtcgaccgcg ctcgcggtgg cacgagcgtc tcgatcttgc
     9541 gatcgccgca tgcgagcagg ccgaccttgt cctgctgctg gatcacgaga tacgcgagcg
     9601 cggcgagcgc gagccccgca tactcgagct tcgcgatgcc accgcccgcg aacgtcatcg
     9661 acgccgacgc gtcgagcacg aggtagacgg tgagctgcga ctcttgctcg tactgtttga
     9721 cgtagtagcg atcgcctttc gcgtacgcgc gccagtcgat gtggcgcagc tcgtcgccgg
     9781 gcgagtactc cttgtgctcg gagaactcga cggacgaacc gtgcacgctc gcgcggtgca
     9841 ggcccgacag cgcgccctcg acgatgacgc gcgccttgat cggaagcgag cctagtctcg
     9901 cgagctgcgc aggatcgatc ttctgctgca gcatcgagtg cgcctatggc gtgacgacgg
     9961 acagcagccg gtcgacgagc tcaccggcgc gctgcccttc ggcctcggcg cgatagttga
    10021 ggatcaggcg gtgctgcagc gtcggacgcg cgagcgcagc gacgtcctcg atcgcgacgg
    10081 cgaaccggcc gtcgaggatc gcgcgcgcct tcgcggcgag gatcaaaaac tgcgacgcgc
    10141 gcggacccgc gccccacgac accgcgtcgc ggatgaacgg cggcgcgttt ggcgccgacg
    10201 gccgcgtcga gcgcgcgagc gcgaccgcgt acttcacgac gtgatcggcg gctggcacgc
    10261 gacgcacgag ctcttggagc tgcaggatgc gctgcggcga cagcacgcgc gacagctccg
    10321 ggcgatggtc gctcgtctgc tggcgcacga tctcttcttc ttcggcctcg ctcggataat
    10381 cgacatcgac ctggaacatg aagcgatcga gctgcgcttc gggcagcggg tacgtgccct
    10441 cttgctcgat cgggttctgg gtcgcgaaca cgaggaacgg cagcgggaga tcatgcgtcg
    10501 tgccgccggc ggtgacacgg aactcctgca tcgactgcag cagcgcagcc tgcgtcttcg
    10561 gcggcgtacg gttgatctcg tccgcgagca cgaggttcgc gaacaccggg cccttgagga
    10621 agcggaacac gcgccgaccg gtcgtgtggt cttcctcgag cacctcggta ccggtgatgt
    10681 cggccggcat gaggtcgggc gtgaactgga tgcgctggaa cgtgaggttc agcgtctcgg
    10741 cgagcgtcga gatcaacagc gtcttcgcga ggccgggcac accgacaaac agcgtgtgac
    10801 cacgcgcgaa cagggcgacg agcagctgat cgacgacgcg gcgctggccg atgatgcgct
    10861 tctcgatctc ggtgacgatc tgcttgtgcg cgtcggcgag ctcggccacc gcctcgaggt
    10921 cggacctcga ggtcgccgga tcggacggga ccgtggggtt cacctcggtc gtggcttctt
    10981 gccgaatagg agtaatcggc attatgtcgg cgggcttggt catctagttc ttcagctggt
    11041 cacacgcggt ccgctcccgg gcagaccgag aatctcc
//
LOCUS       DASUFX010000465         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1880239, whole genome shotgun sequence.
ACCESSION   DASUFX010000465 DASUFX010000000
VERSION     DASUFX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880239"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3186)
                     /locus_tag="VFV99_18655"
     CDS             complement(<1..3186)
                     /locus_tag="VFV99_18655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5061399.1"
                     /translation="MLGVATASAQSITTGAVQGRVTDKDTGLALPGVTVTIGSQVTNT
                     DENGNYKITELIPGTYEVVFEFDTTRAVRRGIVVSANGVTSLFHPLKIGEAVFIDGSP
                     PPINIVSQAKETRIGRKAIESLPTGPTFEATLRNVAGTQNDGVGIAVSGSSALENRYL
                     VDGIDITGLTYGDVGTSLLNDFIHEVQVVSGGYNAEYGRSTGGVVNIVTRSGTDEFRG
                     SVFGVVTPGFLTAKRDAAPSNASSIDVTGDNAYNGHFGFELGGPIIKKRAWFYIGMAP
                     QLARTNYTRVTKSQTDCRVRMDNGELSTCSPANADTDPDVDPKTGFYITDEIDREVRS
                     ATAKSAQVITKINVAATPDDQLQLSLIAVPNKSESPALFGLPATGTRTWGLTTDTAAR
                     WTSKLNKGATEIEALGAWHRSTANSGSIDPAYDDVPLQQLYSVDLVKMAALGGESAAT
                     AAGCQDFPGNPNDKYPLITNCPNNFNAYAVGGPGPLARDLEERRAGRFGLLHRIKAAG
                     THELKGGLDFEDNRKTKARLYSGGALIQNLGNSIQVNRYAELARPGENDPKFDRMCTI
                     SNDSSTGASMGGELVPCRYLGGLDDPSTRVEGHSVNWGAYLQDSWHPLQNLTFNAGMR
                     YEEQRLYYAERLRGTPNPLTGEPLGDTAMKLRGNWSPRLGAIWDPSGEAKSKIYGAWG
                     RYYEGIPMDINDRSFGGEVTLQQTYSPASCGPIDPKLGQVDGATCLGKTPESETMIGA
                     NGTLIAPGIKAQYMDEALLGGEIALPSNVVLGAVLQYRRLGRVIEDVSTDNAQTYLIA
                     NPGEWSREEEAKLVHQIATTTDKLLKGRLEEQLRMFQGVRIFDKPRRDYAAIELTVSR
                     KFISGLYLSGSYTYSRAVGNYPGLVSYDNGQIDPNISSQYDLIELLANRAGKLPQDRP
                     HYIKLDAYRDFDLGNESVLTVGGRVRALSGIPQNALGAHYLYGADESFLLPRGQIGRT
                     EFEHGIDLHLGYKRKLTQGSTAELYVDVFNLYDRQGTFRVDETYAPQYSVSVAGAGGI
                     EQNVNPISGGTYEDLLWAKTIDRNGVESSTPI"
     gene            3365..>3555
                     /locus_tag="VFV99_18660"
     CDS             3365..>3555
                     /locus_tag="VFV99_18660"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5061400.1"
                     /translation="MRCREKAAREIPKRFTGQTYWARPISGFGDPRARLLVVGLAPAA
                     HGGNRTGRVFTGDRSGDWL"
BASE COUNT          578 a         1127 c         1153 g          697 t
ORIGIN      
        1 gatcggcgtc gagctttcca cgccgtttcg atcgatcgtc tttgcccaaa gcaaatcctc
       61 gtaagtgcca ccgctgatcg ggtttacgtt ctgttcgatc ccgccggcgc cggccactga
      121 aaccgagtac tgaggcgcat acgtttcgtc gaccctgaac gtgccttggc ggtcgtagag
      181 gttgaacaca tccacgtaca gctccgcggt agatccctgg gtcagctttc gcttgtagcc
      241 gaggtggaga tcgatcccgt gctcgaactc ggtgcggcca atctggccgc gcggcagcaa
      301 gaacgactcg tcggcgccgt agaggtagtg cgcgccgagc gcgttttgcg ggattccgga
      361 cagcgcacgc acgcggccac cgacggtgag cacgctctcg ttgccgagat cgaagtcgcg
      421 gtatgcgtcg agcttgatgt agtgcggacg atcctgcggc agcttgccgg cgcggttcgc
      481 gagcagctcg atcaggtcgt actgcgacga gatgttcgga tcgatctgac cgttgtcgta
      541 cgacacgagg ccggggtaat tgccgaccgc gcgcgagtac gtgtacgagc ccgacaggta
      601 gagacccgag atgaacttgc gcgacaccgt gagctcgatc gcagcgtagt cgcggcgcgg
      661 tttgtcgaag atgcgaacgc cctggaacat ccgcagctgt tcttcgagcc ggcccttgag
      721 cagcttgtcc gtggtcgtcg cgatctgatg cacgagcttg gcttcttctt cgcgcgacca
      781 ctcacccggg ttcgcgatca aatacgtctg cgcattgtcg gtcgacacgt cttcgatcac
      841 gcgaccgagg cgccgatact gcagcaccgc accgaggacg acgttcgacg gcagcgcaat
      901 ctcgccgccg aggagcgcct cgtccatgta ctgcgccttg atgccaggcg cgatcagcgt
      961 gccgttcgcg ccgatcatcg tctcgctctc gggtgtcttg ccgaggcagg ttgcgccgtc
     1021 gacttggccg agcttcggat cgatcggacc acacgacgcc ggcgagtacg tttgctgcaa
     1081 cgtgacctcg ccgccgaacg agcggtcgtt gatgtccatc gggatgcctt cgtagtagcg
     1141 cccccatgct ccgtagatct tcgacttcgc ctcgcccgac ggatcccaga tcgcaccgag
     1201 gcgcggcgac cagttgccgc gtagcttcat cgcggtgtca ccgagtggtt cgccggtgag
     1261 cggattcggc gtgccgcgca gccgctccgc gtaatacagc cgttgctcct cgtagcgcat
     1321 gccggcgttg aacgtcaggt tctgcagcgg atgccacgag tcctggaggt acgcgcccca
     1381 gttgacggag tgaccctcga cacgcgtcga cggatcgtcg agcccaccga ggtagcggca
     1441 cggcaccagc tcgccgccca tcgacgcgcc ggtgctcgag tcgttcgaga tcgtgcacat
     1501 ccgatcgaat ttcggatcgt tctcgccggg tcgcgcgagc tcggcgtagc ggttcacctg
     1561 gatcgagttg ccaaggttct ggatgagcgc gccgccggag tagagccgcg ccttcgtctt
     1621 gcggttgtcc tcgaaatcga ggccgccttt gagctcgtgc gtgccggccg ccttgatgcg
     1681 atggaggagg ccgaagcgac cggcgcgccg ttcttcgaga tcgcgcgcga gcggaccggg
     1741 cccgccgacc gcatatgcgt tgaagttgtt cgggcagttc gtgatcagtg gatatttatc
     1801 gttcgggttg ccgggaaaat cctggcaccc ggccgccgtc gccgcggact cgccgccgag
     1861 tgcggccatc ttcacgagat ccacgctgta cagctgctgg agcggcacgt cgtcataggc
     1921 cgggtcgatc gagccgctgt tcgccgtcga gcggtgccag gcgccgagcg cttcgatctc
     1981 ggtcgcgccc ttgttgagct tcgacgtcca gcgcgcggcg gtgtcggtgg tgaggcccca
     2041 cgtgcgcgtg cctgttgccg gtagaccgaa caacgctggg ctctcgctct tgttcggcac
     2101 cgcgatcagg ctgagctgga gttgatcgtc gggtgtcgcc gcgacgttga tcttcgtgat
     2161 cacctgcgcc gacttcgcgg tcgcgctgcg cacctcgcga tcgatctcgt cggtgatgta
     2221 gaagccggtc ttcggatcga cgtcgggatc ggtgtcggcg ttcgccggcg agcacgtcga
     2281 gagctcaccg ttgtccatgc gtacacggca gtcggtctgg ctcttcgtca cgcgcgtgta
     2341 attggtacgc gcgagttgcg gtgccatgcc gatgtagaac cacgcgcgct tcttgatgat
     2401 cggaccgccg agctcgaagc caaagtgccc gttgtacgcg ttgtcgccgg tgacatcgat
     2461 cgacgacgca ttgctcggcg cggcatctcg cttcgccgtc aagaagccgg gcgttacgac
     2521 gccgaacacc gagccgcgaa actcgtcggt gccggagcgt gtgacgatgt tgacgacgcc
     2581 gccggtcgag cggccatact cggcgttgta gccaccgcta acgacctgca cctcgtggat
     2641 gaagtcgttg agcagtgacg tgccaacgtc gccgtaggtc aggccggtga tgtcgatgcc
     2701 gtcgacgaga taacgattct cgagcgccga tgaacctgac accgcgatgc cgacgccgtc
     2761 gttctgcgta ccggcgacgt tgcgcagagt cgcctcgaac gtcggcccgg tcggcagact
     2821 ctcgatcgcc ttgcggccga tccgcgtctc cttcgcttgc gacacgatgt tgatcggtgg
     2881 cggcgacccg tcgatgaaca cggcctcacc aattttcagt gggtgaaaca gtgacgtgac
     2941 accgttcgcg ctgacgacga tgccgcggcg gaccgctctc gtcgtgtcga actcgaacac
     3001 gacctcgtac gtgccgggga tgagctcggt gatcttgtag ttgccgttct cgtcggtatt
     3061 cgtgacctgc gagccgatcg tcacggtcac gccgggaaga gcgaggccag tatccttgtc
     3121 ggtgacgcgg ccttgcacgg cacccgtcgt gatcgattgt gcgctcgcgg ttgcaacgcc
     3181 aagcaccaga agcaaacacc acactgcgtt tcgcatcccc tcaccgataa aggacagaaa
     3241 cgctccgaga cacgacgcga cgatcaagac ctgtcacaca ctgcgtgcga cactcgcgaa
     3301 agtgtcgcag cgacttcatg cgctgaccga agacatcacc ggttgccgca ggtgcagtcg
     3361 gctcgtgcgt tgtcgcgaga aagcggcgcg cgagattccg aaacgcttta ccggccagac
     3421 gtattgggcg cggccgatca gtggcttcgg cgatccgcgc gcgcgtctgc tcgtcgtcgg
     3481 tctcgcgccg gcggcacatg gtggcaatcg caccggccgc gtgttcaccg gtgatcgctc
     3541 gggcgactgg ctcta
//
LOCUS       DASUFX010000466         6179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1880410, whole genome shotgun sequence.
ACCESSION   DASUFX010000466 DASUFX010000000
VERSION     DASUFX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880410"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="gcvPB"
                     /locus_tag="VFV99_18665"
     CDS             <1..1462
                     /gene="gcvPB"
                     /locus_tag="VFV99_18665"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5061401.1"
                     /translation="AFLTHPTFNRYDSEHEMLRYLHRLAEKDLSLTTSMIPLGSCTMK
                     LNATTEMIPVTWPEFGRMHPFAPAEQWGGYRAMFEQLEGWLAEITGFAAVSLQPNSGA
                     QGEYAGLLAIRAYHNARGDKHRTVCLIPQSAHGTNPASAVMPGMQVVVVATAADGTID
                     LKDLRAKAEQHSKNLSAIMVTYPSTHGVFEEGITELCAIVHQHGGQVYMDGANMNAQI
                     GLTRPAECGADVCHLNLHKSFCIPHGGGGPGMGPIAVAKHLAPYLPGHPLAPPASQPV
                     GPVSAAPWGSASILPISFAYIAMMGEPGLRRATEIAILNANYLAKRLGTHYPVLYTGR
                     EGRVAHELILDCRGFKKTASIEAEDIAKRLMDYGFHAPTMSFPVPGTLMVEPTESESK
                     AELDRFVEAMIAIRQEIAAIESGQMDKVNNPLKNAPHTAAAVASTEWDRPYTREQAAY
                     PVPYLRTHKYWPPVARVDNAYGDRNLVCACPPVDAY"
     gene            1489..2751
                     /locus_tag="VFV99_18670"
     CDS             1489..2751
                     /locus_tag="VFV99_18670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061402.1"
                     /translation="MDLVGLVAVLGAFAVPLYALRARHEERKRRLELEAGKGGGGEDA
                     ETLREENRLLRERVENLESIVCSVDFELNQKVAKLIDEQRSLVLAPGNPGPMHPKGTS
                     SPGGSGAHVAAAQADAKSDAKPNPEALDRTATAHGVKPSLTTTLEPGQVLAQRYRVQR
                     LLGKGGMGAVYLADDEVLGELVALKVISSAFAADEQAMIQRFRREAAAARKVSSPAVI
                     RIHDLGEARPGLLYLSMEYFAGRTLADVIAQRGIVPIKDVQDILQQIGSGLEAAHQAG
                     VVHRDLKPSNVLVGERGAVKIIDFGLATTGLADGLTATGAILGTPHYMAPEQVRGKAV
                     DVRTDIYALGALAYHLVCGRPPFSGDNAIAIGFAHLSEAPAPPRQLRKDCPAKLESAI
                     LAALAKSPDERPSSAKAFLDKAFAASAA"
     gene            2748..3341
                     /locus_tag="VFV99_18675"
     CDS             2748..3341
                     /locus_tag="VFV99_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061403.1"
                     /translation="MNRLALIVAIDALAGCWGGGKPPDALSNANHTPAARRTFEMRLD
                     GVGPLGWKSEATEAALRIALPELTIKTNDLGAESGIVFDVFDGTEKLMYVVPDEQERD
                     ETGTHHYDKTIFSIFATSPRISVSGHSWRVGYPLENANDLMGCECWGEGEVTACTIRA
                     HLAVIFEENCEVARDAGPQAMVGHKIGRIMWKRVADD"
     gene            4069..4248
                     /locus_tag="VFV99_18680"
     CDS             4069..4248
                     /locus_tag="VFV99_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061404.1"
                     /translation="MLEHLDRHHAIEAHSRKVERADVGGAHGDKTAGGGAGGRTWFDG
                     FDYASASIGADTPAT"
     gene            complement(4255..5106)
                     /locus_tag="VFV99_18685"
     CDS             complement(4255..5106)
                     /locus_tag="VFV99_18685"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX5061405.1"
                     /translation="MRPDVSALSPPATRSTEVTVDKLRLADSILRVAVGCWFTVTLVG
                     QWLFMYYVVALYGASTVSGHFEDWMNKPLFRGYIRGDTVGNFSFAAHVLFAAVVTFGG
                     IVQLVPQIRARASAVHRWVGRAFLLATTAVGASGLYMVWVRHASFDVIHSCAVSLNAV
                     LTLVFTTLAWRTARGRDIASHRRWALRTFVVANGPGLFVRVAYAGWAVLADGAGTNGE
                     MSGPMNYVFAFAVYLLPLAILELYLRGRDSARPAIRFAIALVVVAVTAYVAIGTYQAT
                     ISRIAVV"
     gene            5262..>6179
                     /locus_tag="VFV99_18690"
     CDS             5262..>6179
                     /locus_tag="VFV99_18690"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5061406.1"
                     /translation="MLRRLAIPLVACGLLGLLDTAHYGYYLHDSGQGIDVVHAFLRES
                     PAWFVWALAVPAVLWWGERFRLEWPPRIRDVFAHLAGAATAVLIFGLFPTLAEHAFGY
                     ARPTQPLWDDVTGGFAYQAPKGAITYVATLGFGYVAAHAERSRQLLQLRIELSKAQLT
                     ALRMQLNPHFLFNTLHTIGALVRDGSSIGAVEMIEKLGDVLRRVLRADGDLEAPLREE
                     IELLRAYLEIEQVRFGDRLQVAWRLDPLSESLPVPPLILQPLVENALRHGLSRRARPG
                     SLMISSRIQDERLELVVTDDGEGLPADLEA"
BASE COUNT         1101 a         2061 c         2060 g          957 t
ORIGIN      
        1 ggcgttcctc acgcatccga cgttcaaccg ctacgactcc gagcacgaga tgctgcgcta
       61 tctgcatcgg ctcgccgaga aggacctgtc gctcacgacg tcgatgattc cgctcggctc
      121 gtgcacgatg aagctcaacg cgactaccga gatgattccg gtgacgtggc ccgagttcgg
      181 tcgcatgcat ccgttcgcgc ccgccgagca gtggggcggc tatcgcgcga tgttcgagca
      241 actcgagggc tggctcgccg agatcacggg cttcgcggcg gtgtcgctgc agccgaactc
      301 cggcgcgcag ggcgagtacg cgggcctgct cgcgattcgc gcctatcaca acgcgcgtgg
      361 cgacaagcac cgcacggtct gcctgattcc gcagagcgcg cacggcacga acccggcgag
      421 cgcggtgatg ccgggcatgc aggtcgtcgt cgtcgccaca gcggccgacg gcacgatcga
      481 tctcaaggac cttcgcgcga aggcggagca gcacagcaag aatctgtccg ccatcatggt
      541 cacgtacccg tcgacacacg gcgtgttcga agagggcatc accgagctgt gcgcgatcgt
      601 tcatcagcac ggcggtcagg tgtacatgga cggcgcgaac atgaacgcgc aaatcggtct
      661 cacccggccg gcagagtgcg gcgccgacgt ctgtcatctt aaccttcaca aatcattttg
      721 tattccgcac ggcggcggcg gtccgggcat gggcccgatc gcggtcgcca agcacctcgc
      781 gccctatctg cccggccatc cgctcgcgcc gccggcgagc cagcccgtgg gcccggtgtc
      841 ggcggcgccg tggggctcgg catcgattct gccgatctcg ttcgcgtaca tcgcgatgat
      901 gggcgagccc ggtcttcgcc gcgcgacgga gatcgcgatc ctcaacgcga actacctcgc
      961 gaagcgactc ggcacgcatt acccggtgct ctacacgggc cgcgaaggac gcgtcgcgca
     1021 cgagctcatc ctcgactgcc gtggcttcaa gaagacggcg agcatcgagg ccgaagacat
     1081 cgcgaagcgc ctcatggact acggcttcca cgcgccgacg atgtcgttcc cggtgccggg
     1141 cacactgatg gtcgagccga ccgagagcga aagcaaagcc gagctcgatc ggttcgtcga
     1201 ggcgatgatc gcgatccggc aagaaatcgc cgccatcgag agcgggcaga tggacaaggt
     1261 caacaacccg ctcaagaacg cgccccacac ggcagccgcc gtcgcgagca ccgagtggga
     1321 tcgcccgtac acgcgcgagc aagccgccta tccggtgccg tatctgcgca cgcacaagta
     1381 ctggccgccg gtcgcgcgcg tcgacaacgc gtatggcgat cgcaatttgg tctgcgcctg
     1441 cccgccggta gacgcctatt aggctgcttt aaggcatagt acggggcaat ggacctcgtc
     1501 ggcctggtgg cggtgctcgg cgcttttgcg gtgccgctct atgcactgcg tgcgcgccat
     1561 gaagaacgca agcgccggct cgagctcgaa gcaggcaagg gcggtggcgg cgaagacgcc
     1621 gagacgctgc gcgaagagaa tcgcctgctc cgcgagcgcg tcgagaacct cgagtcgatc
     1681 gtgtgctcgg tcgacttcga gctgaaccag aaagtcgcca agctgatcga cgagcaacgg
     1741 tcgttggtgc tcgcgcctgg gaaccctggc ccgatgcacc ccaagggtac ttcctcgcct
     1801 ggcggctcgg gggcgcacgt cgcagctgcg caggcggacg caaagtccga cgccaaacca
     1861 aaccccgagg cgctcgaccg caccgcgacc gcgcacggtg tgaagccgtc gctgacgacg
     1921 acgctcgagc ccggccaagt gctcgcgcag cgctatcgcg tgcagcgctt gctcggcaag
     1981 ggcggcatgg gcgcggtcta cctggccgac gacgaggtgc tcggcgagct cgtcgcgctc
     2041 aaggtcatct cgagtgcatt cgccgccgac gagcaggcga tgatccagcg gttccgtcgc
     2101 gaagcggccg cggcacgtaa ggtcagctcg ccggccgtca tccgcattca cgatctcggc
     2161 gaagcgcggc cgggcctgct ctatctgtcg atggagtact tcgccggtcg cacgctcgcc
     2221 gatgtgatcg cccagcgcgg catcgtgccg atcaaggacg tgcaggacat cctgcagcag
     2281 atcggcagcg gtctcgaagc ggcgcaccaa gcgggcgtcg tccatcgcga cctcaagccc
     2341 agcaacgtgc tcgtcggtga gcgcggcgca gtcaagatca tcgacttcgg cctcgcaacg
     2401 accggcctcg ccgacggcct cacggcaacc ggcgcgatcc tcggcacgcc gcactacatg
     2461 gcgcccgaac aggtccgcgg gaaagccgtc gacgtgcgca ccgatatcta cgcgctcggt
     2521 gcgctcgcgt atcacctcgt gtgcgggcgt ccgccgttct cgggcgacaa cgcgatcgcg
     2581 atcggtttcg cgcatctttc cgaggcgccg gcaccacccc gccagctgcg caaggactgc
     2641 ccggcgaagc tcgaaagcgc gattctcgcc gcgctcgcga agagccccga cgagcggccg
     2701 tcgagcgcga aggctttcct cgacaaagcg ttcgccgcga gtgctgcgtg aaccgcctcg
     2761 cgttgatcgt cgccatcgac gcgctcgcgg gatgctgggg cggcggcaag ccacccgacg
     2821 cactgtcgaa cgcgaaccac acgcctgccg cacgccgcac attcgagatg cgactcgatg
     2881 gtgtcggccc actcggttgg aagtcggaag caaccgaggc ggcgctgcgg atcgcgctgc
     2941 ccgagctcac gatcaagacg aacgacctcg gtgccgaaag cggcatcgtg ttcgacgtgt
     3001 tcgatggcac ggagaagctg atgtacgtcg tgcccgacga acaggagcgc gacgagaccg
     3061 gcactcacca ctacgacaag acgatctttt cgatcttcgc gacgagcccg cggatcagcg
     3121 tcagtggtca ctcgtggcgc gtcggctatc cgctagagaa cgcgaacgac ctcatgggct
     3181 gcgagtgctg gggtgaaggc gaggtgaccg cgtgcacgat ccgcgcgcat ctcgccgtga
     3241 tcttcgagga gaactgcgag gtcgcgcgcg atgctggccc gcaggcgatg gtcggccaca
     3301 agatcggccg catcatgtgg aaacgcgtcg cagacgatta gccgtcgacg cgcgcagcgt
     3361 ggctaaccgt aggtcggcgc ccagggaacg tcccgagccg ggatccgttc ctcagccctg
     3421 cgcatcttca gaaaacggac gagatagcgc agaccgatcg cggttgcgag cacgccgagc
     3481 gcaaccccgc cacgcttggg tcccactctg cgcgagagcg ctctcgtccg acagccgaga
     3541 cggtccgcgt aacgtgctgt cgtgtcgccg gcatggctga gcatatgttg gagcttcgtt
     3601 ttcttgtgcc tcatggaggc aagtccttgc agctcatgtt ccgggcgcgt tcgcacgcgg
     3661 cacgagccgg tagtgagcga cgcgccactc gctcccgcgc gcgtagccga acactccgca
     3721 cacgcgagaa agaacacgcg ccagcgctga tacgcgcggc gaccgagcaa cgcttcgacg
     3781 gcggcgcgct cgcgcacgag gttcgcgtac cacgcctcgg cagtccgcgc gtagtgcgtg
     3841 ccgtcgacga gccatcgctc ggcgatccgc acgtcgcgct gaactgtggc aacagatccg
     3901 cgctcggcat gattccgccg gtgaagaact cgcgcgccat ccagtcggtg ctgccgttgt
     3961 catcgaacgg atatgcgaag cgacggtgcg cgaacacgtg cacaacagcg caccatcggg
     4021 ccgcagccac gtcgcgatcc ggcgcaacag catcgcgtag ttgcgcacgt gctcgaacat
     4081 ctcgatcgac accacgcgat cgaagctcac agccggaagg tcgagcgtgc ggacgtcggc
     4141 ggtgcgcacg gtgacaagac ggccggcggt ggcgccggcg gccggacctg gttcgacggc
     4201 ttcgactacg cgtcggcatc gatcggtgcc gacacgccag caacctgacg gtcgtcagac
     4261 gaccgcgatg cgggagatcg tggcctggta ggtgccaatc gcgacgtacg cggtgaccgc
     4321 taccaccacg agtgcgattg cgaatcgaat cgccggacgg gcactatctc gcccacgcag
     4381 atacagctcg aggatcgcga gcggcaacag gtacaccgcg aacgcgaaga cgtagttcat
     4441 cgggccgctc atctcgccgt tcgtgccggc gccatcagcg agcacggccc atccagcgta
     4501 ggcgacacgg acgaagagcc ccgggccgtt cgcgaccacg aacgtgcgga gcgcccagcg
     4561 acggtggcta gcgatgtcgc gtccgcgcgc cgtacgccac gcaagcgtag tgaacaccag
     4621 ggtaaggacc gcattgagac tgacggcgca cgagtggatg acgtcgaagc tggcgtggcg
     4681 cacccagacc atgtagagcc cgctcgcgcc gaccgcggtg gtcgccagca ggaaggcgcg
     4741 tccaacccag cgatgcaccg cgctcgcgcg cgctcggatc tgcggaacga gttggacgat
     4801 gccgccgaac gtgaccacag cagcgaacaa cacgtgcgca gcgaagctga agtttccgac
     4861 ggtgtcgccc cgaatgtatc cgcgaaacag cggcttgttc atccagtctt cgaagtggcc
     4921 ggagacggtc gaagcaccgt agagcgcgac cacgtagtac atgaacagcc actggccgac
     4981 gagggtcacg gtgaaccagc agccgaccgc aacccgcagg atcgaatccg cgaggcgcag
     5041 cttgtcgacg gtgacctccg tggaacgggt cgcgggcggg ctcagcgccg agacgtcggg
     5101 ccgcatgtgc atccggtacc acggccttcg cgcgagggtt ccgctggtgc aacgagtggt
     5161 ggtctggatg caacgagcgg tgcaacgagc agtgcaacga gtggcagtct ggatgcaacg
     5221 agcggtgcgc gatgccgggc atcgttggct atagtggatg cgtgttgcgc cgcctcgcga
     5281 tcccgctcgt ggcgtgcggg ctcctcggcc tgctcgacac cgcccactac ggctactacc
     5341 tgcacgacag tggccagggc atcgatgtcg tgcacgcgtt cctccgcgag tcgccggcgt
     5401 ggttcgtctg ggcgctagcc gttccggcgg tgctctggtg gggcgagcgc tttcgtctgg
     5461 aatggccgcc tcgaatccgc gatgtgttcg cgcacctggc gggcgccgca acggccgtgc
     5521 tgatcttcgg cctgtttccc actctggccg agcacgcatt cggctatgcg cggccgacac
     5581 aacccctgtg ggacgacgtg accgggggct tcgcgtatca ggctccgaag ggcgcgatca
     5641 cgtacgtcgc gacgctggga ttcggctatg tcgcggcgca cgccgagcgt tcgcgtcagc
     5701 tcctgcagct gcgcatcgag ctgtcgaagg cccagctgac agcgctgagg atgcagctca
     5761 atccgcactt cctcttcaac acgctgcaca ccatcggcgc gctggtgcgt gacgggagct
     5821 cgatcggcgc agtcgagatg atcgagaagc tcggcgacgt tctgcgtcgc gtgctgcgag
     5881 cggatgggga cctagaggcg ccgctccgcg aagagatcga gctcttgcgt gcgtacctcg
     5941 agatcgagca ggtgcgcttc ggcgatcgcc tgcaagtggc gtggcggctc gatccgctga
     6001 gcgaatcgct gccggtgcca ccgctcatcc tacagcctct ggtcgagaat gcactgcgcc
     6061 acggcttgtc tcggcgcgcg cggcccggct cgctgatgat ctcgtcgcgg atccaggacg
     6121 aacgactgga gctcgtcgtc accgacgacg gcgaaggtct gccggcggat ctcgaggca
//
LOCUS       DASUFX010000467         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1186395, whole genome shotgun sequence.
ACCESSION   DASUFX010000467 DASUFX010000000
VERSION     DASUFX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186395"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFV99_18695"
     CDS             complement(<1..1095)
                     /locus_tag="VFV99_18695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061407.1"
                     /translation="MKKPTPFGKYYLLERINTGGMAEVFRAKAFGVEGFERLVAVKRI
                     LSNIAEDKEFIRMFIEEAKLAVQLNHANIAQIFDLGVVDGSYYIALEHVHGRDLRAMF
                     DRCRQLGDPMPVSQACFVVMKVCEGLDYAHNKRDQAGRELHLVHRDVSPQNVLVSFEG
                     EVKLIDFGIAKAAGKGSKTQAGILKGKFGYMSPEQVRGIPVDRRSDVFSCGIVLYELL
                     TGERLFVGESDFSTLEKVRNVEILPPSTYNRRIPDELERIVLKALAKDPEERYQNAID
                     LHDELQAFVYTAGEFYSRKDLAGWMKKTFGREIEEETAKLESYRQLKAPVAPEDRVTN
                     PVLGRSPTGSRPPTAAATPSAQRKSTPSGPN"
     gene            complement(1230..2888)
                     /locus_tag="VFV99_18700"
     CDS             complement(1230..2888)
                     /locus_tag="VFV99_18700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5061408.1"
                     /translation="MRSPRHFALSAFGIVIGISTFVVFLASTEQVAAVLEKIFPLEQV
                     QVVAPRASLLGKDISKKLDDNIVKTILTRPEVSLAIPRMNLAFPAFGRGNFEGNDLKF
                     EVGGFADGVDPKFVQDDERTKELFRDWMGPDNENDPHRVACVPPPRTEDDEVIQSPKA
                     PRKDKQNQVPSGWGDTPSAAAGSDTTGSGSAAAATGSGTTGSGSAVATGSAGSAAGSA
                     AGAGAGSATPSGPTIAPIAGKNGEYFNPCPDPDRYYCDDTTARCEHRVPIVMSPTMIE
                     LYNGQFAKSHGLPIVETDFVKWVTQRGGLSAMRFSIGLGQTTIAGSNTTIKRKPRRVE
                     GVLVGVSQKAMPIGMTMPIQYIQRWNDEFMGHEAATTYSSIIVTLKDRNQIAPFAQWL
                     QDTLDLRLEDSLGEKFATVLFVIRLVLVLISLIIIGISAINIAHNFFMQVTERRRELG
                     LLRAVGATQSDVRMVVLGEAALIGLIGGCLGVLFGVAIASGIDYASAHYLPRFPYKPA
                     TWFNFKWWIWMGGLACAAGFAVLGGYLPARRASTMEPAQALTQN"
     gene            complement(2930..>3306)
                     /locus_tag="VFV99_18705"
     CDS             complement(2930..>3306)
                     /locus_tag="VFV99_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5061409.1"
                     /translation="FSKNNRDVQGRGREALRRVGLSDFANRRPGELSGGQKQRVAIAR
                     ALFSSPSLLLCDEPTGNLDSETGREVIDFFRELNAKDGVTLLIVTHERRVSSVAKRVI
                     AMRDGMLVETTDEALAAQAGPA"
BASE COUNT          554 a         1053 c         1070 g          629 t
ORIGIN      
        1 gttcgggccg gatggagtgg acttgcgctg tgcgctcggc gtggccgcgg ccgtgggtgg
       61 gcgcgagccc gtcggcgaac ggccgaggac cgggttggtg acgcggtctt ccggggcgac
      121 cggagctttg agctggcgat agctctcgag cttggccgtc tcttcttcga tctcgcggcc
      181 gaacgtcttc ttcatccagc ccgcgaggtc ttttcgcgag tagaactcgc ccgcggtgta
      241 gacaaacgct tggagctcgt cgtgcagatc gatcgcgttc tggtaccgct cctcgggatc
      301 cttcgcgagc gctttgagca cgatgcgctc gagctcgtcg gggatgcggc ggttgtacgt
      361 cgacggcggc aggatctcga cgttgcgaac cttttcgagc gtcgagaaat cgctctcgcc
      421 gacgaacaag cgctcgccgg tgagcagctc gtacagcacg atgccgcacg agaacacgtc
      481 gctgcggcga tcgacgggga tgccgcgcac ctgctcgggc gacatgtagc cgaacttgcc
      541 cttgaggatt ccggcttgcg tcttggagcc tttgcccgcg gctttcgcga tgccgaaatc
      601 gatcagcttc acttcgcctt cgaacgagac gagcacgttc tgcggcgaca cgtcgcgatg
      661 cacgaggtgc agctcgcgac cggcttggtc acgcttgttg tgcgcgtagt cgaggccttc
      721 gcagaccttc attacgacga agcacgcttg cgagaccggc atcggatcgc cgagctggcg
      781 gcagcgatcg aacatcgcgc gcaggtcacg tccgtgaacg tgctcgagcg cgatgtagta
      841 gctgccgtcg acgacaccga ggtcgaagat ctgcgcgatg ttcgcgtgat tgagctgcac
      901 cgcgagcttc gcttcctcga tgaacatgcg gatgaactct ttgtcctccg cgatgttcga
      961 gagaatgcgt ttgaccgcga cgagccgctc gaagccttcg acaccgaacg ctttcgcgcg
     1021 gaacacctcg gccatgcccc cggtgttgat ccgctcgagt aggtagtatt tgccgaaagg
     1081 cgtcggcttc ttcacgttgc gtttacccgg agcgatgcgc gcgacatcgc tactgtctgg
     1141 gaccctatcc gaattcgagt ccttgggcca caagagcttt atcccccgat gggccgtagt
     1201 ggtacatcgg ggatcgtcgg aattactagc taattctgcg ttagcgcttg cgctggttcc
     1261 attgtcgagg cgcgtcgcgc cgggaggtaa ccgccgagca ccgcgaatcc ggccgcgcag
     1321 gccaatccgc ccatccagat ccaccacttg aagttgaacc aggtcgcggg cttatacggg
     1381 aatcgcggga ggtagtgggc ggatgcgtag tcgatcccgg aggcaatcgc gacgccgaac
     1441 aagacgccga ggcagccgcc gatcaggccg atcaacgcag cttcaccaag gacaaccatc
     1501 cgtacgtcgc tttgcgtggc gccgaccgcg cgcaagagtc cgagctcgcg ccgccgttcg
     1561 gtgacctgca tgaagaagtt gtgcgcgatg ttgatcgcgc tgatgccgat gatgatcagc
     1621 gagatcagca cgagcacgag ccggatcacg aacagcaccg tcgcgaactt ctcgccgagc
     1681 gaatcctcga gccggagatc gagcgtgtct tgcagccact gcgcgaacgg cgcgatctga
     1741 tttcggtcct tgagcgtgac gatgatcgac gagtacgtcg tcgccgcctc gtgccccatg
     1801 aattcgtcgt tccagcgctg gatgtactgg atcggcatcg tcatgccgat cggcatcgcc
     1861 ttctgactga cgccgacgag cacgccctcg acgcgacgtg gcttccgttt gatcgtggtg
     1921 ttgctgcccg cgatcgtcgt ctggccgagg ccgatggaga accgcatcgc cgacaggccg
     1981 ccacgctggg tgacccattt gacgaagtca gtctcgacga tcggcaggcc gtgcgacttc
     2041 gcgaactggc cgttgtagag ctcgatcatc gtcggcgaca tcacgatcgg cacgcgatgc
     2101 tcgcagcgcg ccgtggtgtc gtcgcagtag tagcgatcag ggtccgggca cggattgaag
     2161 tactcgccgt tcttgcccgc gatcggcgcg atggtcggcc ccgacggggt cgcgctgcca
     2221 gcacccgcgc ccgccgcact gcctgcggcc gagcctgcag agccggtagc gaccgcggaa
     2281 ccagaacccg tggtgcctga ccctgtagcc gctgccgcgg aaccagaacc cgtggtgtct
     2341 gaccctgcag ccgcggacgg cgtatcgccc cacccgctcg gcacctggtt ctgcttgtcc
     2401 ttgcgcggcg ccttcggcga ctggatcacc tcgtcgtcct cggtgcgcgg cggcggcacg
     2461 cacgcgacgc gatgcggatc gttctcgttg tcgggcccca tccagtcgcg gaacagctct
     2521 ttggtgcgct cgtcgtcctg gacgaacttc ggatcgacgc catccgcgaa gccgccgacc
     2581 tcgaacttga ggtcgttgcc ctcgaagttg ccgcggccga atgccgggaa cgcgaggttc
     2641 atgcgcggaa ttgccagcga cacttcgggg cgcgtgagga tcgtcttgac gatgttgtcg
     2701 tcgagcttct tgctgatgtc cttgccgagc agcgatgcgc gcggcgcgac gacctgcacc
     2761 tgctcgagcg ggaaaatctt ctcgagcacc gcagcgactt gttcggtcga cgcgaggaac
     2821 acgacgaacg tcgagatgcc gatcacgatg ccaaacgccg acagcgcgaa atgccgcggg
     2881 ctgcgcattg tgttcgatgc gaccattttc gcgagattgc caacgggcat catgcgggac
     2941 ctgcctgcgc cgcgagcgct tcatccgtgg tctcgacgag catgccgtcg cgcatcgcaa
     3001 tcacgcgctt ggccacgctc gacacgcgtc gttcgtgggt gacgatgagc agcgtcacgc
     3061 catcctttgc attgagctcg cggaaaaaat cgatcacttc gcggccggtc tcgctgtcga
     3121 ggttgccggt cggctcgtcg cacaacagca agctcggcga cgagaacagt gcgcgcgcga
     3181 tcgcgacgcg ttgcttctga ccgcccgaca gctcgccggg ccggcgattc gcgaagtccg
     3241 agagcccgac gcggcgaagt gcctcgcggc cgcgaccctg cacgtcgcgg ttgttcttcg
     3301 agaacg
//
LOCUS       DASUFX010000468         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_353434,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000468 DASUFX010000000
VERSION     DASUFX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353434"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFV99_18710"
     CDS             complement(<1..806)
                     /locus_tag="VFV99_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061410.1"
                     /translation="MRAVAIVIAVLASVAPAYADDAAAKALFDEGKTLFAEGKYGEAC
                     AKLEASFKLSAMSSTRGLLGACYEKVGKLASAWAAYRDSAAIADRQGHAERAQAAREK
                     ATELEAKLAHLAIDTTAAKTVTGLEVTIDGVVQPAAALDTSLPIDAGPHVIVADARGY
                     TKWTTTVDIVDGEKQQAVVPMLVANPTELEEATVEHTMSTRRKIALAVTGGGVAVLGA
                     SAVFGILAKVNWNSSNCNGDTKTCPDAAAQEKADAARRDATIATVLGIAGV"
     gene            complement(803..>2293)
                     /locus_tag="VFV99_18715"
     CDS             complement(803..>2293)
                     /locus_tag="VFV99_18715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061411.1"
                     /translation="PLIGTVVGGRYEIVRLIGKGGMGAIYEVRNTRLGRQFALKTLTG
                     DAAANPEVLLRFRREADVIAKIKHPNIVEVIDWESLADGSPAMVMEYLHGEDLGERIK
                     EGPLRWPQIARIADQVLAALSVAHANGIVHRDLKPQNIFLAQDDSGDERVKLLDFGVS
                     KVRDSKSLVTTDARLLGTPAYMSPEQSEGRAEDVGTHSDVWAMGAILYEMATGEVPFD
                     ADNLPAILYKICHSKAAPITKKRADAPEPFIELVDETLERDISKRISDATVLRVRLRE
                     ALRNLAGVQFVDTLPGLRTSAQMAASGRRKRMTDAMSDTIGAITPPKGNLAVGTPVEK
                     SLKVGTENTLLATKDSPSVIARKKWRPAAVVIGALAIAMVAGAVVIATQRGGKSTPAA
                     ATPVAKPADQTPQIDAGAVVATPPPAPDAAVAVAADPADPPKPDPKQRPIKKKDPKTN
                     TAKIDPKQDPAKTEPKQDPVKTEPKQEPAKKKCAKDDFECLYGDGT"
BASE COUNT          316 a          795 c          718 g          464 t
ORIGIN      
        1 acaccggcaa tgccgagcac cgtcgcgatg gttgcatcgc gccgtgccgc gtccgctttc
       61 tcctgcgcgg ctgcgtccgg gcaggtcttc gtgtcgccgt tgcagttcga gctgttccag
      121 ttgaccttcg cgaggattcc gaacaccgcg ctggcgccga gcaccgcaac gccaccgccg
      181 gtaaccgcga gcgcgatctt gcgccgcgtc gacatcgtgt gctcgaccgt cgcctcctcg
      241 agctccgtcg ggtttgcgac gagcatcggc accacggcct gttgcttctc accgtcgacg
      301 atgtcgaccg tcgtcgtcca cttcgtgtag ccgcgcgcgt cggcgacgat cacgtgcggg
      361 ccggcatcga tcggcagcga cgtgtcgagc gccgccgcgg gctgcacgac gccgtcgatc
      421 gtcacttcga ggccggtcac cgtcttcgcc gcggtcgtat cgatcgcgag gtgggccagc
      481 ttggcctcga gctccgtcgc tttctcgcgg gccgcctgtg cacgttcggc atggccttgc
      541 cgatccgcga tggccgccga gtcgcgatac gcggcccacg cgctcgcgag cttgccgacc
      601 ttctcgtaac acgcgccgag caggccgcgc gtcgacgaca tcgccgacag cttgaagctc
      661 gcttcgagct tcgcgcacgc ttcgccatac ttgccttcgg cgaacagcgt cttgccttcg
      721 tcgaacagcg ctttcgccgc cgcgtcgtcc gcatacgcgg gcgccacgct cgcgagcacc
      781 gcgatcacga tcgcgaccgc tctcacgtgc cgtctccgta caagcattcg aagtcgtcct
      841 tcgcgcattt cttctttgcc ggctcctgct tgggctccgt cttcaccgga tcttgcttcg
      901 gctcggtctt cgccgggtcc tgcttcggat cgatcttcgc cgtattcgtc ttcggatcct
      961 tcttcttgat cggcctttgc ttcggatctg gtttcggcgg atccgctggg tcggctgcga
     1021 cagcgacagc cgcgtcgggc gcaggtggcg gcgtcgcgac cacggcacct gcatcaattt
     1081 gtggcgtctg gtcggctggt ttcgcgaccg gagtggccgc agccggcgtg ctcttgccgc
     1141 cacgctgcgt agcgatcacg accgcgccgg cgaccatcgc gatcgcgaga gcgccgatca
     1201 ccacggccgc ggggcgccac ttcttgcgtg cgatcacgct cggggagtct ttggttgcga
     1261 gcagcgtgtt ctcggtgccg accttgagcg atttctcgac gggcgtaccg accgcgaggt
     1321 tgcccttcgg tggcgtgatc gcgccaatcg tgtcggacat cgcatcggtc atccgcttgc
     1381 gccggccgct cgccgccatc tgggcgctcg tccgcaggcc gggcagcgta tcgacgaact
     1441 gcacgcccgc gagattgcgc agcgcttcgc gtaagcgcac gcgcagcacc gtggcatccg
     1501 agatccgctt gctgatgtcg cgctcgagcg tctcgtcgac gagctcgatg aacggttccg
     1561 gcgcatcggc gcgcttcttc gtgatcggtg cggccttcga gtgacagatc ttgtagagaa
     1621 ttgcaggcag gttgtcggcg tcgaacggca cctcgccggt tgccatctcg tagaggatgg
     1681 cgcccatcgc ccacacatcg ctgtgagtgc cgacatcctc cgctcgtccc tctgactgct
     1741 ccggagacat gtatgccggc gtgccgagca aacgtgcatc cgtcgtgacc aggctcttcg
     1801 aatcacgcac cttcgagacg ccgaagtcga gcagcttcac gcgctcgtca cccgagtcat
     1861 cctgcgcgag gaagatgttc tgtggcttga ggtcgcgatg cacgatgccg ttcgcgtgcg
     1921 cgaccgacag tgcggcgagc acttgatccg cgatgcgtgc gatctgcggc cagcgcagcg
     1981 gaccttcttt gatgcgctcg ccgagatcct cgccgtgcag atactccatc accatcgcgg
     2041 gcgagccgtc ggccagcgat tcccagtcga taacctcgac gatattcggg tgtttgatct
     2101 tcgcgatcac gtcggcttcg cgacggaatc gcagcagcac ttcggggttc gccgccgcgt
     2161 cgccggtcag tgtcttgagt gcgaactgac ggccgagccg cgtattgcga acctcgtaga
     2221 tcgcacccat gccgcccttg ccgatcaggc gcacgatctc atagcgaccg ccgacgacag
     2281 tgccgatcag cgg
//
LOCUS       DASUFX010000469         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_74585,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000469 DASUFX010000000
VERSION     DASUFX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74585"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1893
                     /locus_tag="VFV99_18720"
     CDS             64..1893
                     /locus_tag="VFV99_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5061412.1"
                     /translation="MGKRFFAVVVVIASACTALHYSTAYQESVVVSQSSYTFPGPGSQ
                     PFTISAGGSGADDNLVNVRLEPNCSTSVWSLWLDPTKPFPYRICGGAILNFNASGSGT
                     SVCPTTYTFYANFNASLPGLSTCAVAIDTMSNLGSSSETQYINLSGSGSGVSGISAMP
                     SSIDFADVQINTTSTASSVTVKNLSLVSGTTVTGTLTGTGFVVTGSYTSFGLGPGSSV
                     SFPVTCHPTVVGPLTGRLEFAGAGSSAGTKLDCNGIDSTVTIAPSQINFDPTLVGRAP
                     PRKTVGISGASTAVIENVSLDSAAQMAGVTITMNPQGQQVGGGKTIELDYSAMTAHEA
                     GPLGTLSVKVSVDQNPRMVNLAGTALIGGVGTNPATVEFGAVCAGSKVMKDVEVYANE
                     AGDVSIVSLTKPAAPFDAMAVEPLPKMVMGGHSGPSVTVRASLMPTGAGEVRDAFALV
                     SNVPNHDPYEVQLHGTGLASGIAATPDLVHFGTAAAGTTTSIKEVQFTNCGTSDLMFN
                     GAALAGRDHGEFTIIGANPPRVLHPTESEIIMVVMQPESPGEKQAQLVISHDQGTTPV
                     DLDGTGEGGANKERETYYACSTGRSGSLWLIALALLALRRRRR"
     gene            1922..>3737
                     /locus_tag="VFV99_18725"
     CDS             1922..>3737
                     /locus_tag="VFV99_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061413.1"
                     /translation="MSLAARLRDHFARRGEWWWVPAFYTLAVIWIYRDLWHQHGVATG
                     LGWDTIDTHGPDLDFFSTEIREGRFSLWNPYDKGGYPLFCDPVFDRYNPFNWPFGIIG
                     ALFGSSWWLVQIKVLAHHIVAGALMHLFMRSRGLSVRSALVGGLGLIASAPLLTHKAS
                     NILWPLVWVPLVWLAIDYALARPSWRRGAALAAALVPCITAGSPPGLFYAALMIAPYA
                     IWRAVTTLRQPERRTRDALVQLAICVGVAAVLAMLVAAVTVLPTQELVALGSRDRFAP
                     PGRAFALALSLPLPAAARGVFCRGAGLFELYMGVAVVVLAVMALAVRPRFDRGIAVCL
                     VLTACLGIVLAAGATGGVLPFLVDHVPGFAMLRVPGRYKLLAAWTLAASAGYGIGALE
                     AAWGDRGLRRRALIVAGVALVIAVIFVVGWGQPASPKDRPAWWSIAMTAIVAGFIVAA
                     VSAPKRWAEAALGALALCALLDAPAFTFVLPHAPPASDIRRTHDDDDRYIPRFEGARD
                     RFRIYDEFVLGERAGARLRLRDFRGYPALDPISLRRYVDVLDYAKKDSAIVTDFNVRW
                     ALIKPHFRYGSSTSFVMPNSVAFESRGDSLWEAKHPAPL"
BASE COUNT          543 a         1217 c         1283 g          694 t
ORIGIN      
        1 aggcgtcgct cccacgcgcc ccgtcgaatt gactggctac ctggctgagg cgatccttat
       61 gccgtgggga aacgcttctt cgcggtggtc gtggttatcg cctcggcctg caccgcgttg
      121 cactactcga ccgcctacca agagtcggtc gtcgtctcgc agtccagcta cacgtttccg
      181 gggccaggat ctcagccgtt cacgatcagt gcgggcggct cgggcgcgga cgacaatctg
      241 gtcaacgtcc ggctcgagcc caactgcagc acgtcggtgt ggtcgctgtg gctcgacccg
      301 acgaagccgt tcccctatcg tatctgtggc ggcgcgatcc tgaatttcaa cgcgagcggc
      361 agcggcacgt cggtgtgccc gacgacgtac acgttctacg cgaacttcaa cgcgtcgctc
      421 cccggtctgt cgacgtgcgc ggtggcgatc gatacgatga gcaacctcgg cagtagcagc
      481 gagacccagt acatcaacct gtcgggctcg ggcagcggcg tgagcggtat ctcggcgatg
      541 ccgagctcga tcgactttgc cgacgtgcag atcaacacga cgagcaccgc gtcgtcagtg
      601 acggtcaaga acctcagcct ggtcagcggc accaccgtga cgggtacgct gaccggcacc
      661 gggttcgtcg tgaccggcag ctacacgagc ttcggcctcg gccccggttc gtcggtcagc
      721 ttcccggtga cctgccatcc gactgttgtg ggtccgctga ccggcaggct cgagttcgcc
      781 ggcgctggct cgtcggccgg cacgaagctc gactgcaacg gtatcgactc gacggtgacg
      841 atcgcgccct cgcagatcaa cttcgacccg acgctcgtcg gtcgcgcgcc gccgcgcaag
      901 acggtgggca tttccggtgc cagcaccgcg gtgatcgaga acgtgtccct cgactcggcg
      961 gcgcagatgg cgggcgtgac gatcacgatg aacccgcaag gccagcaggt cggcggcggc
     1021 aagacgatcg agctcgacta ctccgcgatg acagcgcacg aggccgggcc gctcggtacg
     1081 ctgtcggtta aagtctcggt tgatcagaac ccgcgcatgg tgaacctcgc cggtacggca
     1141 ctcatcggcg gcgtgggcac gaatccggcg accgtcgagt tcggcgcggt atgtgcgggc
     1201 agcaaggtga tgaaggacgt cgaggtctac gcgaacgagg ctggcgacgt ttcgatcgtg
     1261 tcgctgacga agccggccgc gccgttcgat gcgatggcgg tcgagccgct gccgaagatg
     1321 gtcatgggcg gtcactcggg cccgagtgtc actgttcgcg cgtcgctgat gccaaccggt
     1381 gcgggcgagg ttcgcgacgc gttcgcactc gtcagtaacg taccgaacca cgatccgtac
     1441 gaggtgcagc tgcacggcac cgggctggcc agcggcatcg cggcgacgcc ggacctcgtg
     1501 cacttcggta ccgcagcggc tggtacgacg acatcgatca aggaagtgca gttcacgaac
     1561 tgcggaacct cggacttgat gttcaacggc gcggcgctcg ccggtcgcga ccacggcgag
     1621 ttcacgatca tcggcgcgaa tccgccgcgc gtactgcatc cgacggagtc cgagatcatc
     1681 atggtcgtga tgcagccaga atcgccgggt gagaagcagg cgcagctcgt gatctcgcac
     1741 gatcagggca ccacgccggt cgatctcgac ggtaccggcg aaggcggcgc gaacaaggag
     1801 cgcgagacgt actacgcctg ctcgacgggg cggagcggct cgctgtggct catcgcgctc
     1861 gcattgctcg cgttgcgccg ccgtcgccgt tgagcgcgga tttccgggct agactgccgg
     1921 cgtgagccta gccgcgcgcc tgcgagacca tttcgcgcga cgtggcgaat ggtggtgggt
     1981 gccggcgttc tacacgctcg ccgtgatctg gatctatcgc gacctctggc atcagcatgg
     2041 cgtcgcgacc gggctcggct gggacacgat cgacacgcac ggtcctgacc tcgacttctt
     2101 ctcgaccgaa attcgcgagg gccggttctc gctgtggaac ccgtacgaca agggcggcta
     2161 tccgctgttc tgcgatccgg tgttcgatcg ctacaaccca ttcaactggc cgttcggaat
     2221 catcggcgcg ctgttcggca gctcgtggtg gctggtccag atcaaggtgc tcgcgcatca
     2281 catcgtcgcc ggtgcgctca tgcacctgtt catgcgctcg cgcgggctct cggtgcggtc
     2341 ggcgctggtc ggtggcctcg ggttgattgc atcggcaccg ctgctcacgc acaaggcgtc
     2401 gaacatcctg tggccgctcg tctgggtgcc actcgtgtgg ctcgcgatcg attacgcgct
     2461 cgcaagaccg agctggcggc gtggtgcggc gctcgcggcg gcactcgtgc cgtgcatcac
     2521 cgcgggctcg ccaccgggat tgttctacgc ggcgcttatg atcgcgccgt atgcgatctg
     2581 gcgtgccgtg acgacgctgc ggcagcccga gcgacgcaca cgcgatgcgc tcgtgcagct
     2641 cgcgatctgc gttggcgtgg cagcggtgct cgcgatgctc gtcgctgcgg tgacggtgtt
     2701 gccgacgcag gagcttgtcg cgctcggctc gcgcgatcga tttgcgccgc cgggtcgtgc
     2761 gtttgcactc gcgctgtcgt tgccgcttcc cgctgccgcg cgcggtgtgt tctgtcgcgg
     2821 tgccggtctg ttcgagctgt acatgggcgt tgccgtcgtc gtgctcgcgg tgatggcact
     2881 cgcagtgcgg ccacgcttcg accgtggcat cgcggtctgt ctcgtgctga ccgcgtgtct
     2941 tggcatcgtg ctcgccgccg gtgcgacagg cggcgtgctg ccatttctcg tcgatcacgt
     3001 gcccggtttc gcgatgctgc gcgtgcccgg ccgctacaag ctactcgccg cgtggacgct
     3061 cgccgcgagc gcgggctatg gcatcggcgc gctcgaggcg gcgtggggcg atcgcgggtt
     3121 gcgccgccgc gcgctgatcg tcgccggtgt cgcgctcgtc atagcggtga tcttcgtcgt
     3181 cggctggggg cagccggcat cgccgaagga tcgacccgca tggtggtcga tcgcgatgac
     3241 ggcgatcgtt gcgggcttca tcgtcgccgc ggtgtcggca ccgaagcgat gggcagaagc
     3301 ggcgctcggt gcgctcgcgc tgtgtgcgct gctcgatgcg ccggcgttca cattcgtgct
     3361 gccgcacgcc ccgccggcgt cggacatccg ccgcacgcac gacgatgacg accgctacat
     3421 cccgaggttc gagggtgcgc gcgatcggtt tcgcatctac gacgagttcg tgctcggcga
     3481 gcgcgcgggt gcgcgactgc ggctccgcga cttccgcggt tatccagcgc tcgatccgat
     3541 cagcctgcgc cgctacgtcg acgtgctcga ctacgcgaag aaagattcgg cgatcgtcac
     3601 cgacttcaac gtgcgctggg cgttgatcaa gccgcacttc cgttacggca gctcgacgag
     3661 cttcgtgatg ccgaatagcg tggccttcga gtcgcgcggc gattcgctgt gggaggcaaa
     3721 gcatccggcg ccgctcg
//
LOCUS       DASUFX010000470         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1604761, whole genome shotgun sequence.
ACCESSION   DASUFX010000470 DASUFX010000000
VERSION     DASUFX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604761"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFV99_18730"
     CDS             <1..343
                     /locus_tag="VFV99_18730"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5061414.1"
                     /translation="EIPRRTALLVAETKARGGRVIAIGTTVVRALEAAAHPSIGSFHA
                     GSGVATLRLDGSYKLRVVDGLVSGLHAPGESHFDLLSAFAPRERLTRAIDLAAQHGLS
                     SHELGDACLIL"
     gene            384..1022
                     /locus_tag="VFV99_18735"
     CDS             384..1022
                     /locus_tag="VFV99_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061415.1"
                     /translation="MFSLKALAIVVTGVVAAAGAVGNALWGPRAKARKRLQRGTAAIA
                     DREIVTLVGTVRQRGELLVAPLSGKECVLFESLGHVKELRSGSRYADTVAELRQHQMV
                     PFELDTGDEIVVVDGTAADLELPPTPVVPRRIEREKAFLEAGGQPFKLVISSGFDETR
                     VAPGDRIAVQGMAIIELDPTATDERGYRESAPRKIRLVAHDAHPLTIGRARR"
     gene            complement(1026..1757)
                     /locus_tag="VFV99_18740"
     CDS             complement(1026..1757)
                     /locus_tag="VFV99_18740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX5061416.1"
                     /translation="MNLLLIDPGEIRGDGTCTVFDRRAHHMRTVVGVQIGSTVRAGLL
                     GGAVGTAEVIADDGSALTFRLNLTEPPPKPLDVELLLAVPRPKVITRTIEIVASFAVK
                     RIDLTNAWRVDKSYLRSPRLDPGALALAARFGAEQGATTYLPTITLHDRLMAVLDDRF
                     ANPDPSQTRLIAHPSAPPIESVVTKRAPTVLAIGPEGGWIEREIETFVERGFQPVSLG
                     TPILRVEAAVASSLGQLLLLNRMRA"
     gene            1788..3440
                     /locus_tag="VFV99_18745"
     CDS             1788..3440
                     /locus_tag="VFV99_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5061417.1"
                     /translation="MPFFQEPPRLGNQFDDDPLLPSWIARHIPEAHHGEVATELHALG
                     DLAVEYYAKQQLDRLNEPVLTQWDPWGNRIDRIDVSPLWVEAQVLAARHGMVAAGYET
                     RLGAQARTHQFAIVHVLGPSLDVYSCPLAMTDGAARTLLSSGNKALIDRYVPLLTSRD
                     PATMWTSGQWMTERTGGSDVSQSETIAKLDGDQWRLYGTKWFTSATTSNMALTLARPE
                     GNGDGSRGLALFFVELRDGNGRLRNIQVNRLKDKLGTRKVPTAELTLDGTPATLVGTA
                     SDGVRQITPMLSITRTWNAISAVSAMRRGLALARDFARRRKAFGALLVDKPLHADTLA
                     GLEAEYAGAFCLAFRSAHLIGILEHGNDEQAERLARALTPIAKLTTGKQAVAVTSEAI
                     ESCGGAGYVEDTGLPRILADAQVLPIWEGTTNVLSLDTLRALGKGGALEAIAAEVESC
                     TAAATDAGLAKPALAARSALRHATAWVQDAMPQATRLEAGARRFALTLGRSLELALLC
                     QHAQWCLDHGHGPRLAAAARRFAQNGVDLIGDSSTDDTKLLV"
     gene            complement(3527..3874)
                     /locus_tag="VFV99_18750"
     CDS             complement(3527..3874)
                     /locus_tag="VFV99_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061418.1"
                     /translation="MIELQGDRGKAMEQANQEMAAHCGPNNYTIVQEGEEAIGTDTYS
                     REDTSAAATQSRSGRTQYADSTTQGQTSTRTATAWRVHYQCNGMAGGPPPGQPAQPAA
                     GPAGPPPPPPGSY"
     gene            4074..>5062
                     /locus_tag="VFV99_18755"
     CDS             4074..>5062
                     /locus_tag="VFV99_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEX5061419.1"
                     /translation="MAREVVSVESRIEQVTVYASGARVRRVASLRAPLPPAVRFVGLP
                     LAVIDDTVRCEVEGAPVVTAVRVGVDTPTGEPRAEESDELRAARQRVALADSEVTRLR
                     AALEKLAAAPLIEDDPSDEAPAAWAAIVAARRELVAMRAKRELTLREQLAAALRELDD
                     AQRALEVVIEREARASTAKAARLHEVRKYAELEIAGSGDAIVCVEYQIAAARWAPSYV
                     ARLDAEQARFELRAVVAQASGEDWTDVALRLSTAEPERFAQLPELQPQKIGRRQAEPA
                     RRGFRAPPAGADVLYSDYDRSFPRRAEPITTEIFDDSTYEGRMPAPSTVPAKT"
BASE COUNT          854 a         1684 c         1729 g          795 t
ORIGIN      
        1 cgagattccg cgtcgcaccg ccctactcgt cgccgagacg aaggcgaggg gaggccgcgt
       61 gatcgcgatc ggcacgacgg tcgtgcgcgc cctcgaagca gcggcgcatc cgagcatcgg
      121 cagctttcat gccggctcgg gtgtcgcaac gctgcggctg gacggcagct acaagttgcg
      181 tgtcgtcgac ggtctcgtca gcggcctgca cgcgccaggc gagagccact tcgatctatt
      241 gagtgcgttc gcgccgcgcg agcggctgac gcgcgcgatc gacctcgctg cccagcacgg
      301 gctgtcgagt cacgagctcg gcgacgcttg cctgatcctc tgagtcgtcc gggccgcgtc
      361 gattcgggta cagtcgggcg cagatgttct cgttgaaggc gctcgcgatc gtggtcactg
      421 gcgtggtcgc ggccgcaggc gctgtcggca atgcgctgtg gggccctcgc gcgaaagcac
      481 gcaagcggct gcagcgcggc accgcggcaa tcgccgatcg cgagatcgtc acgctcgtcg
      541 gtactgtgcg gcagcgcggc gagctcctcg tcgcgccgct atccgggaag gaatgcgtac
      601 tgttcgagtc gctcggccac gtcaaagagc tgcggtcagg ttcgcgctac gcggacacgg
      661 tcgcggagct acgccagcac cagatggtgc cgttcgagct agataccggc gacgaaattg
      721 tcgttgtcga cggtacggcc gcggatctcg agctgccgcc gacgccggtt gttccgcgtc
      781 ggatcgagcg cgagaaagca tttctcgagg caggcgggca gccattcaag ctcgtcatct
      841 cgtccggctt cgacgagacg agagttgcgc ccggtgaccg gatcgcggtg cagggaatgg
      901 cgatcatcga gctcgatccg accgctacgg acgagcgcgg ctatcgcgag agcgcgccgc
      961 gcaagatccg gctcgtcgcg cacgacgcgc atccactgac gatcgggcgc gcacgccgct
     1021 gacggtcagg cgcgcatacg gttcaacagg agtagctggc cgagcgacga tgcgaccgcc
     1081 gcctcgacgc gcaagatcgg agtgccgagt gacaccggct ggaagccgcg ctcgacgaac
     1141 gtctcgatct cgcgctcgat ccatccgcct tctggaccga tcgcgagcac ggtcggcgcg
     1201 cgcttcgtga cgacgctctc gatcggcggc gcgctcgggt gcgcgatgag tcgcgtctgg
     1261 ctcgggtcgg gattcgcgaa gcgatcgtcg agcaccgcca tcaggcgatc gtggagcgtg
     1321 atcgtgggca ggtacgtggt ggcgccttgc tcggcgccga accgcgcggc gagcgcgagt
     1381 gcgcccggat cgagacgcgg cgagcgcagg tagctcttgt cgacacgcca tgcgtttgtg
     1441 agatcgatac gcttcaccgc gaacgatgcg acgatttcaa tcgtgcgcgt gatgaccttg
     1501 gggcgcggca cggcgagcaa cagctcgaca tcgagaggtt tcggaggcgg ctccgtcagg
     1561 ttcaggcgaa atgtcagcgc cgaaccgtcg tcggcgatca cctcggcggt cccgaccgcg
     1621 ccgccgagta gacccgcacg caccgtcgag ccaatctgga ctccgacgac ggtgcgcata
     1681 tggtgcgcgc gccggtcaaa gaccgtgcaa gtcccgtccc cgcgtatctc gcccgggtcg
     1741 atcaacaaca gattcacgtt acgaccttac cttggtaggt tcccgccatg ccgttctttc
     1801 aggaaccgcc tcgtctgggc aaccaattcg acgacgatcc cctgctgcca tcgtggatcg
     1861 ctcgccatat tccagaggcg caccacggcg aagtcgcgac cgagctccat gcgttaggtg
     1921 acctcgcagt cgaatactac gccaagcagc agctcgatcg gctcaacgaa cccgttctga
     1981 cgcagtggga cccgtgggga aaccgcatcg atcgcatcga tgtgtcgcca ctgtgggtag
     2041 aggcgcaggt gctcgcagcc cgccacggca tggttgcagc tggttacgag actcggctcg
     2101 gcgcgcaggc gcgcacgcat cagttcgcga tcgtccacgt gctcgggccc tcgctcgacg
     2161 tctactcgtg cccgctcgcg atgaccgacg gcgcggcacg cacgctgctg tcgagcggca
     2221 acaaggcgct gatcgatcgt tatgtaccgc tactgacgtc gcgcgatccg gcaacgatgt
     2281 ggacgagcgg ccagtggatg accgagcgca ccggcggcag tgacgtttcg cagagcgaga
     2341 cgatcgcgaa gctcgacggc gatcagtggc gactctacgg cacgaagtgg ttcacgtcgg
     2401 cgacgacgtc gaacatggca ctcacgctcg cgcgccccga gggcaacggc gatggcagcc
     2461 gcgggctcgc actgttcttc gtcgagctgc gcgatggcaa cggccggctg cgcaacatcc
     2521 aggtcaatcg cctcaaggac aagctcggca cgcgcaaggt accgaccgcc gagctcacgc
     2581 tcgacggcac gccagcgacg ctggtcggca cggcaagcga tggcgttcgc cagatcacgc
     2641 cgatgctctc gatcacgcgc acctggaacg cgatcagtgc ggtgtcggcg atgcgtcgcg
     2701 gcctcgcact cgcgcgtgac ttcgcgcgcc ggcgcaaggc attcggcgca ctgctcgtcg
     2761 acaagccgtt gcacgccgat acgctcgccg gcctcgaagc cgagtacgcg ggtgcgttct
     2821 gcctcgcatt ccgcagcgcg catctgatcg gtatcctcga gcatggtaac gacgaacaag
     2881 ccgagcggct ggcgcgcgcg ctgacgccga tcgcgaagct gacgaccggc aagcaagcgg
     2941 tcgccgtgac atccgaagcg atcgaatcct gcggtggcgc aggctacgtc gaggacaccg
     3001 gactgccgcg catcctcgcc gacgcgcagg tgctgccgat ctgggagggc acgacgaacg
     3061 tgctgtcgct cgacacgcta cgtgccctcg gcaagggcgg cgcgctcgaa gcgattgcgg
     3121 ccgaagtcga gagctgcacg gccgcggcga ccgatgccgg tctcgcgaag ccggccctag
     3181 ccgcacgctc ggcgcttcgc cacgcgaccg cgtgggtgca ggacgcgatg ccgcaggcca
     3241 cgcgcctcga agcaggtgca cgccgcttcg cgctcacgct cggccgcagc ctcgagctcg
     3301 cgctgctgtg ccagcacgcg cagtggtgcc tcgatcatgg ccacggtccg cggctagcgg
     3361 cggccgcgcg tcggttcgcg cagaacggtg tcgatctgat cggcgactcg tcgaccgatg
     3421 acacgaagct gctcgtttga gaaaaggggt ctgaccagaa aggggtcaga cccagttccg
     3481 aaaacgaaaa aggccccgtt ggggcctttc gcgctatcgc ctcggcttag tagctgccgg
     3541 gcgggggcgg cggcggacct gccggacccg cggcgggctg cgcaggctgg cccggaggcg
     3601 gaccacccgc catgccgttg cactgatagt ggacgcgcca cgcggtcgca gtgcgcgtgc
     3661 tggtctggcc ttgcgtcgtc gagtccgcgt actgcgtgcg gccgctacgc gactgcgttg
     3721 cagcagccga cgtgtcctcg cgcgaatacg tgtccgtgcc gatcgcctct tcgccttctt
     3781 gcacgatcgt gtagttgttc ggaccgcagt gcgcagccat ctcctgattc gcttgctcca
     3841 tggccttgcc gcgatcgccc tgtagctcga tcacgccgcc agcctgcgta cgctgaatca
     3901 cgcgcgcgct gccacaagcg gccagcgcca gaaccaaacc aagtgaaagc ttccgaagca
     3961 tgcggtttcc tctctccgat tgacggccgt agacgggggc aaagtaaacc atcggcatcc
     4021 cttgggcaac agccgcttga cactccgagg ccgcgagacc gaccctgcca gacatggcgc
     4081 gtgaggtggt ctccgtcgag tcacggatcg agcaagtcac ggtctacgcc agtggcgcac
     4141 gggttcgacg agtcgcgagc ttgcgggcgc cgttgccgcc tgccgtgaga ttcgtcgggt
     4201 tgccactcgc cgtgatcgac gacaccgtgc ggtgcgaggt cgaaggtgca cccgtagtaa
     4261 ccgctgttcg cgttggtgtc gatacgccga ccggcgaacc gcgtgcggaa gaatctgacg
     4321 agcttcgtgc ggcgcgacaa cgcgtcgcgc tcgctgacag cgaagttaca cgcctgcgcg
     4381 cggcgctgga gaaactcgca gcggcgccgc tcatcgaaga cgatccgagc gacgaggcgc
     4441 ctgcagcgtg ggcagcgatc gtggctgcgc ggcgcgagct cgtcgcgatg cgcgcgaagc
     4501 gcgagctgac gttgcgcgaa caactcgcgg ccgcgttacg cgagctcgat gatgcacagc
     4561 gcgcactcga ggtcgtgatc gagcgcgagg cacgtgcgag caccgcgaag gcagcgcgcc
     4621 ttcacgaggt gcgcaaatac gccgagctcg agattgctgg cagcggcgac gcgatcgtgt
     4681 gcgtcgagta tcagatcgcg gccgcgcgat gggcgccgag ctacgtcgcg cggttggacg
     4741 cggagcaggc gcgcttcgaa ctgcgcgcgg tcgtcgcgca agcatcgggc gaagattgga
     4801 cggacgtcgc gctgcgactg tcgaccgccg aacccgagcg gttcgcgcag ctgcccgagc
     4861 tgcagccgca gaagatcgga cgcaggcaag ctgagcccgc gcgccgcgga tttcgagcgc
     4921 caccggcggg cgccgacgtt ctctacagcg actacgaccg gagcttcccg cgtcgcgccg
     4981 agccgatcac gaccgaaatc ttcgacgact cgacgtacga aggccgcatg cccgcgccgt
     5041 cgacagtgcc cgcgaagacc ga
//
LOCUS       DASUFX010000471        15206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1604775, whole genome shotgun sequence.
ACCESSION   DASUFX010000471 DASUFX010000000
VERSION     DASUFX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604775"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1365)
                     /locus_tag="VFV99_18760"
     CDS             complement(292..1365)
                     /locus_tag="VFV99_18760"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5061420.1"
                     /translation="MGLHTLTRSRRSRERSAMQHVAIDLGSKESQVCIRGADGTLLQE
                     RKHPTRKLGELMQTWPTSRVVLETSSESFRIADLALAAKHEVRVVPATLAKQLGVGER
                     GVKTDLRDARKLSEVSCRIDLPSVHIPSADARELRSMLRSRETLVSTRTKLVNHVKGW
                     MRTQLWKLRSGAPATLPDRLRAYAASVQQPLPAHIEETLVVLEALAIQINASNRRVKK
                     LAKEHSVARLLMTIPGVGPVTSLTFIAAVDRVDRFERTHHLQSYLGLTPGENSSSERE
                     RRTSITKAGATSVRRTLVQAAWTAIRAVPNAPMVKWAAQIAERRGKYIAVVALARKMA
                     GIMFALWRDNTTYSAFKAAASLQ"
     gene            1401..1868
                     /locus_tag="VFV99_18765"
     CDS             1401..1868
                     /locus_tag="VFV99_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX5061421.1"
                     /translation="MFRDDAATTSVLQDAKNAKNAKARSLRPEPSPEVDALAHAVIGA
                     AIEVHRQLGPGFLEHVYEAAMSIELTARRIPFQRQVTFPVYYKHVLVAESRIDLLIAE
                     QLVVELKAVDELRPIHAAQVLSYLRAGEFQLGLLFNFNVYSLRQAIRRVVWTG"
     gene            complement(1976..3853)
                     /locus_tag="VFV99_18770"
     CDS             complement(1976..3853)
                     /locus_tag="VFV99_18770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX5061422.1"
                     /translation="MTDSVLAQAFVYLCAAVVFVPIAKRLGLGAVLGYLIAGVVIGPW
                     VLGLVGDSHHSVMHFSEFGVVMMLFVVGLELRPALLWELRRSIFGLGTAQVVGCAAAV
                     AGAAIALGVDWQPAIAVGLTFAMSSTAIVLSTLSERGLLKSSGGQGSFSVLLFQDISV
                     IPILAVFPLLGVAVASETEGGRPAWQSALLLIGAVVGVVVIGRYLVRPLFQFLAGAKL
                     RESFTAAALLIVVGIALLMQTAGLSPALGTFLAGVLLADSEYRHELETDIEPFKGLLL
                     GLFFITVGAQLDFGLIGSMPLTIAGMVLGTMVLKLAVIRLLGKIFGLDRPARWLLAFA
                     LAQIGEFAFVLLQFGVGNHVFSDAFAKPLIAVVAISMVLTPPLFIVLERWVLPIVSER
                     GAKREQDVIKHADAPVVIAGYGRFGQIVGRILRASGFPLTILDLDPGVIEMLSNLGVK
                     AYYGDASRIDLLHAAGCEHAKLFILAVDDPDEATKIAEHVRHNFPHLPIIARCRNRPH
                     YWQLRKLGIEHVFRETFAASWESGICALRVLGYRAFTAQRLGQRWRQHEESIIEEFRR
                     YEEEDDDATYWEKARAAMGEAERLMRQEHPTRLEEDGWDNESLRADQNVDAAAAEQQT
                     S"
     gene            complement(3850..4467)
                     /locus_tag="VFV99_18775"
     CDS             complement(3850..4467)
                     /locus_tag="VFV99_18775"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5061423.1"
                     /translation="MRRVLVLFAHPVLERSRVNRRLVDGIRDIEGVTIRDLYEEYPTL
                     SIDVKREQADLLAHDVIVFQHPFYWYSCPALLKEWQDLVLEHGWAYGKGGTQLRGKIT
                     LNVITTGGPATAYQRGGYNRFTIRELLSPWDQTAHLCGMRFLAPFAVHAALKVVSDAD
                     VTGNLGGYRQLLEALRDDRIDLDAAAAAQNLALELDRVLAPREVA"
     gene            4570..5058
                     /locus_tag="VFV99_18780"
     CDS             4570..5058
                     /locus_tag="VFV99_18780"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5061424.1"
                     /translation="MQPSIAERFFFGRPYHPVAGDTVVPVVPRSGTALLGRILIAAIF
                     LISGIAKLTDPAGTIGYMHQAGISHADTLVYVAGIAELAGGLGILFGFLTRLAALGLF
                     VFLAIVQPYFHKFWGLPAEEAKNAMVQFFKDLSLMGGLLMLVAMGPGRYSLDAKMRRR
                     AA"
     gene            complement(5700..5816)
                     /locus_tag="VFV99_18785"
                     /pseudo
     CDS             complement(5700..5816)
                     /locus_tag="VFV99_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(6086..8818)
                     /locus_tag="VFV99_18790"
     CDS             complement(6086..8818)
                     /locus_tag="VFV99_18790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5061425.1"
                     /translation="MSNESEKGKPDDKAKPAAKEDAKAEAKPEKKVKGADGPPPPLDE
                     RRLKYAELIPYGGTFLPAELVAHLADGRAIVRANAALALAAANHPASELVHLLRDSEI
                     SVGLAGAEAITRLGPLARPLIPGITQAAEGMKPEVTEAIVAALADLVGKADEEMTLGL
                     DVPLDNAIKSVLEACKVVGKAGIAFLTRATRHERSRIRVNAIAGLGKLGKTDADTAMA
                     CLTQIEASDPVPDVRTAAKQAMLAVVAREKVAAVDSLPKNIPDFEARKLTNSELSEYA
                     DVIDIDQMIFALQDGRDHVKVNAGRALAAKGAKAGRAATAMGLLMRDSVASVRRETAK
                     SLGKLGVEAVAAAPDLVGALGDSDGEVAEAASDTLEALGERALEALVKGLEAGGEEHG
                     RRVAALIVKLPRAADILTDQFKNSPAVNVQVNAAAGLGMFGKSITAAGLAALHGARTG
                     GDVRTRTAVRAALDIIETRGDTGPKQVSIGGFEDRFLTLAELDKVKADVEKAGVGDLT
                     AYLTDGRDVVRANAALGLGVLGAASQGSALALGVLLRDDAAKVRLGAAQALDRIGDAA
                     VTETADYLVGALRDSDEKVAETVAVVLRARKTKMIGALVRGLEADDPRHATRIVEVIN
                     VLADAMEILCDAFESPAVNVQVNAALGLGMLGRDRVGKGRKKLEGARTGGWERTREAV
                     RKALDMLDGPRKTGPDDVAVEGFEERILEPAAFADPAKLRVDDLAQHLIDGRPVVRAN
                     AATALGAVGAAAFGTLSGIAVLLRDDDMRVRIAAANSIDKLGDDAVREAAGFLVGSLR
                     GDADVAKASGKVLGARKAKVLGALIKGLETDDETQARRILELVNALPDACEILCDAFE
                     SPAENVQVNAAMGIGMLGEKRAGTSGRKKLEGARTGGFARTREAVFKALAALKG"
     gene            complement(8846..9652)
                     /locus_tag="VFV99_18795"
     CDS             complement(8846..9652)
                     /locus_tag="VFV99_18795"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5061426.1"
                     /translation="MTVNTRMPVAYLPHGGGPWPFVDMGWNLTDTDELAAYLRSVAAL
                     PKTPPKALLVISAHWEERVPTVMTSPQPPMLYDYYGFPPASYEIQWPAPGEPALAARV
                     QELLAKAGIPSATDAKRGFDHGTFIPLKLTYPDANVPTVQLSLKAGLDPEEHIVIGRA
                     LAPLRDEGVFIIGSGLSFHNLRVFRDPRAKPIAETFDAWLRDAATREQAERDRALIDW
                     SRAPAARESHPREEHLIPLMVIAGAAGNDRGTVAFNGTFGGLRLSAYHYG"
     gene            complement(9714..11135)
                     /locus_tag="VFV99_18800"
     CDS             complement(9714..11135)
                     /locus_tag="VFV99_18800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5061427.1"
                     /translation="MARVFLVNPPSPEPVHTPLLSFCHLAASLRAAGHDVGLLDASAP
                     NAPHSHEDIHLFVAAFAPDLVGIHLKTLHVQPAYALAEALAGFTLVAGGPHATIVPDE
                     PLARGFRYAIRGEGEDTLVELADAIDTMRRRDTGRDMRSIDDISGLSWIDRGMVRHNP
                     ARPFNTDLDRLAPPLSALDLFDPRWYPALPGTALAPAGILSSRGCPAACTFCSNDVTG
                     RKFRYRSARSVVEEVALLRGRGLIGFSFFDDSFAVGRRRVRELCDAITTLGTQVWWTC
                     TAHPAHLDRDVLADMKRAGCSGIDIGMESADPGMLVRIGKGVTVERVLDVLAWCKELE
                     IHTVVNLMFGWPDETDAELDATIGFLDRAAPLAGGFNARGVVVPYPGTQIYDQNHARF
                     GFTGWWIREAPLAYPAFPTRWDEAEIRRAYATDPALDRNFFQHPPSRVARIRDALALK
                     AEATLAITQRRVSNSGVPAAGAR"
     gene            complement(11140..13341)
                     /locus_tag="VFV99_18805"
     CDS             complement(11140..13341)
                     /locus_tag="VFV99_18805"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5061428.1"
                     /translation="MDVFGGALSAGGDFFRDAAVAAAHELGVFAALGQPRTVGDVATE
                     IGVGARRLRRLLDVLVSFDVLARIRDRYAAAHVPERPVVPPAGWGVLANVIRSDNPLA
                     LGDTVTTQRMHEHLVSAGAPAAAELVTIIAGLAPLRRLLDLGGGAGAYTRAWLAARSD
                     ATATLVDVPDVVALARDTLAPFGDRAAFIAGDMRAVQLGESYDVALLANVLHLHDESI
                     GAALCELAAKAIAPGGVVVVKDLRMDEGRAGPREGLVFALNMALYTPGGDVYESSRIR
                     SWLIAAGLVDVEERRLATSPDAIVVIGRKPAEDATARELDSELSRIGKIAWRDLEAEG
                     RLRADAHPRRLAYPAPLRRVLARAVADDSDDIADNADIRRHYTEIMPRMRVTQICGTD
                     EPAATLMHSPLDWARLPRMSAALDRLFALLVECGVDAELPLGAASAAELRASAPTFGE
                     LYMRTHYGACMPLLYGYPADLAYFMSRGLELHATIDRYLTAPLVHELCHFGRYRDALP
                     MHLDECVAGYLGVRVWPEFAYPQSGHDDAIYASPWLAQVGQAVVRAFGLGAVVRGHTG
                     HSLWSAVLPSAFLDAAMRQAADDWRARRTLHFLSDTFAPGPWVELALRHATTGDDPAF
                     DRRIVKDALRAMCLDNLQVAGSFRTRSRMPGTITIDAVARTVTTAYQSAVDPLPPSYW
                     LPPAVAARLVANGIAAYSLELSDLGAIPEAATAICEAAKNCERAGFALVIR"
     gene            complement(13341..13691)
                     /locus_tag="VFV99_18810"
     CDS             complement(13341..13691)
                     /locus_tag="VFV99_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061429.1"
                     /translation="MSGSPKEKLQTLSGAEEVSDEREVYLRDGRKLVVSEQGGEQLVE
                     IRNESGMLEVRIRLTEQGPVLQMESAKLSLKATEAVEIESKQVAIKATEKIDVEAKGD
                     VVVIGAMIHLNPDK"
     gene            13727..14683
                     /locus_tag="VFV99_18815"
     CDS             13727..14683
                     /locus_tag="VFV99_18815"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5061430.1"
                     /translation="MTLDLLVCPGCRTLSGDRLDVSTLDPAGDVLACECGRRYPIVDD
                     VPIVMTDATTYLRNEVATIVERDMAPEVAALLVAPGPDEAPYPRMLEHLSIYLDAHWG
                     DRATPPPGFALAPFVERIGALPKVSMAVELGCSVGRVLAELPAERAVGLDMHFGAVRR
                     ARRLLSGESLAYARREVGRHYSTATAAGKRTADRKLVCADVLDPPLLPDVWDRVVALN
                     MLDSVAHPRQLLSVIDGLCTLGGEIIVSSPYAWQSAVLADHERFGGADPATALRTLLT
                     EGTGLRARYRIEDEAEILWTLRRDARSAVTYRTHYIRARKGT"
     gene            14745..>15206
                     /locus_tag="VFV99_18820"
     CDS             14745..>15206
                     /locus_tag="VFV99_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061431.1"
                     /translation="MQQLARLKFTSFNLKVPKDWRDPSGGDAGDHYSRAFKPDEKAST
                     PGMPPLLQPASLNKYHTDTQKMHIAKVGAFIDGTCSAICSAWSQWQTAATMVGVVITA
                     ITASVGQVVGPPWTPLIMASAPKASPMELKYSNVIATVLGTAWLQYTATIKI"
BASE COUNT         2546 a         5151 c         4934 g         2575 t
ORIGIN      
        1 agatggaacg actatgtagt ggcggtcgcg tcaagcgcag acctgtcccg tccggggctt
       61 gaggtcccgg cacatcgttc gtgttgatcg gcccggtggt cggctgtcca ggaaggtgga
      121 tgtcgggttg tcgcagtcga ctattcgcgc tcggtggcgc agtagcgggg tccgtcgacg
      181 acgtcacatt cgccaagcca ggctgcgcat cagcatgctg gtgcggccta cgttccgatc
      241 gcgtgatcgc cgtcgttgga caaccttcat ccgggtcctt agtggtttcg gctactgcag
      301 ggacgctgcc gccttgaagg cgctgtacgt cgtgttgtcc cgccagagcg cgaacatgat
      361 gccggccatc ttccgagcca gagcgacgac cgcgatgtac ttgccgcggc gctccgcgat
      421 ctgcgccgcc catttcacca tcggtgcgtt cggcacggcg cgaatcgcgg tccacgccgc
      481 ctgcaccagc gtgcgtcgta cggacgtcgc gccggccttt gtgatgctcg tccgtcgctc
      541 gcgctccgag ctcgagttct cgcccggtgt cagtcccagg tacgactgca gatggtgcgt
      601 ccgctcgaag cggtccacgc ggtccacagc cgcgatgaac gtcagcgagg tcaccggtcc
      661 gacgcctggg atcgtcatca gcagacgcgc taccgagtgc tccttggcga gcttcttcac
      721 gcgccggttc gacgcattga tctgaatcgc cagcgcttcg agcactacta gtgtctcctc
      781 gatgtgtgcg ggtagcggtt gctgcacgct tgccgcgtag gctcgcagtc gatctggcag
      841 cgtcgccggc gctccgctgc gcagcttcca cagctgcgtt cgcatccagc ccttcacgtg
      901 attgacgagc ttggtcctcg tgctcacgag cgtctccctg cttcgcagca tcgaacgaag
      961 ctcgcgagca tccgccgacg ggatgtgcac cgacggcaag tcgatcctgc acgacacctc
     1021 gctcagcttc cgtgcgtccc gaaggtcggt cttgacgccg cgctcgccca cgccgagctg
     1081 cttggccagc gtcgccggaa ccacccgcac ctcgtgcttc gctgccaacg ccaggtccgc
     1141 aatccgaaac gattcggacg acgtctccag caccacgcgg ctcgtgggcc aggtctgcat
     1201 cagctctccg agcttcctcg tcggatgctt tctctcctgt agaagcgtcc catccgcgcc
     1261 gcggatgcaa acttgcgact ccttcgaacc cagatcgatg gcaacatgct gcatggctga
     1321 cctttctcga ctgcgcctcg agcgcgttaa tgtgtgaagc cccattctgg gtgacttcgt
     1381 tgtcggggag gtcagccact ttgtttcgag acgacgccgc cactacatcc gttctccaag
     1441 atgccaagaa cgccaagaac gccaaggcaa gaagccttcg accggagccg tcgcctgagg
     1501 tcgatgcgct ggcccatgcg gtcatcggcg cggccatcga ggtgcatcga caactcggcc
     1561 ccggctttct cgagcacgtc tacgaagcgg cgatgagcat cgagctcacg gcgcggcgga
     1621 ttccgtttca acgccaggtg acgttcccgg tctactacaa gcacgtcctc gtcgcggagt
     1681 cgcgcatcga tctgctcatc gccgaacagc ttgtagttga gctgaaagcc gtcgacgagc
     1741 tgcggccgat ccacgcggca caggtgctct cgtatctaag ggcgggcgag tttcagctcg
     1801 ggctgctctt caatttcaac gtgtactcat tgcgtcaggc gatccggcgc gtggtttgga
     1861 ccggatagat cttcttacct tggcgttctc ggcggccttg gcctcttggc gttccatctg
     1921 gggagccgcg ctcggacggc gctcctcgcg ctcggccggt cctcacggcg tcgtatcagc
     1981 tcgtctgctg ctcggccgcc gccgcgtcga cgttctgatc ggcgcgcagc gactcgttgt
     2041 cccagccgtc ttcttcgagg cgcgtcgggt gctcctggcg catcagccgc tcggcttcgc
     2101 ccatcgcagc acgcgccttc tcccagtacg ttgcgtcgtc gtcttcttcc tcgtagcgcc
     2161 ggaattcttc gatgatcgac tcctcgtgct ggcgccagcg ctgcccgagg cgttgcgcgg
     2221 tgaatgcgcg gtaaccgagc acgcgtagcg cgcagatgcc gctctcccac gaggctgcga
     2281 acgtctcgcg gaacacgtgc tcgatgccga gcttgcgcag ctgccagtag tgcggccgat
     2341 tgcggcagcg cgcgatgatc ggcaggtgcg ggaagttgtg ccgcacatgc tcggcgatct
     2401 ttgtcgcctc gtccgggtcg tcgacagcga ggatgaacag cttcgcgtgc tcgcacccag
     2461 ccgcatgcag caggtcgatg cgcgacgcat cgccgtagta ggccttcacg ccgaggttag
     2521 agagcatctc gatcacaccc ggatcgagat cgaggatcgt cagcgggaag ccgctcgcgc
     2581 gcaggatgcg gccgacgatc tgcccgaacc ggccgtaacc ggcgatcacg accggcgcgt
     2641 cggcgtgctt gatcacgtcc tgctcgcgct tcgcgccgcg ttcgctcacg atcggcagca
     2701 cccaccgctc gagcacgatg aacagcggtg gcgtcagcac catcgagatc gcgacgacgg
     2761 cgatgagcgg tttcgcgaac gcgtcgctaa acacgtgatt gccgacgccg aactgcagca
     2821 gcacgaacgc gaactcgcca atctgcgcga gcgcgaatgc gagcaaccag cgcgccggac
     2881 gatcgagacc gaagatcttg ccgagcaggc ggatcaccgc gagcttgagc accatcgtgc
     2941 cgagcaccat cccggcgatc gtgagcggca tgctgccgat caagccgaag tcgagctgtg
     3001 caccgacggt gatgaagaac aagccgagca agaggccctt gaacggctcg atgtcggtct
     3061 cgagctcgtg gcggtactcg ctatccgcga gcagcacgcc ggcgaggaac gtaccgagcg
     3121 ccggcgatag acctgccgtc tgcatcagca gcgcgatgcc gacgacgatc agcagcgcgg
     3181 ccgcggtgaa cgactcgcgc agcttggcac cggcgaggaa ctggaacaac ggccgcacca
     3241 gataacgacc gatcacgacg acgccgacga cggcgccgat caggagcaac gcgctctgcc
     3301 atgccggcct gccaccttcg gtctcgcttg cgacggcgac gccgagcagt ggaaacaccg
     3361 cgagaatcgg aatgaccgag atgtcctgga acagcagcac cgagaagctg ccctgcccgc
     3421 cgctgctctt gagcaaaccg cgctcggaga gcgtcgacaa cacgatcgcc gtcgacgaca
     3481 tcgcgaatgt gagcccgaca gcgatcgccg gctgccagtc aacgccaagc gcgatcgcag
     3541 caccggcgac ggccgcagcg cagccgacaa cctgcgctgt accgagcccg aagatcgaac
     3601 ggcgcagctc ccagagcagc gcaggccgca gctcgaggcc gacgacgaac aacatcatca
     3661 cgacgccgaa ctcggagaag tgcatcaccg agtggtgcga atcgccgacg aggccgagca
     3721 cccagggacc gatcacgacg ccggcgatca gatagccgag caccgctccg agcccgagcc
     3781 gcttcgcgat cggcacgaag acgaccgcgg cgcagagata gacaaacgct tgggcgagaa
     3841 ccgaatcggt catgcgacct cccgaggagc gagcacgcga tcgagctcga gtgcgaggtt
     3901 ctgcgcggcg gcggcagcat cgagatcgat gcggtcgtcg cgcaacgctt cgagcagctg
     3961 gcgatacccg ccgaggttgc ctgtgacatc tgcatcgctc acgaccttca gcgcggcgtg
     4021 gaccgcgaac ggcgcgagaa accgcatccc gcacagatgc gcggtctggt cccacggcga
     4081 gagcagctcg cggatcgtga accggttgta gccacctcgc tgatacgcgg tggccgggcc
     4141 accggtcgtg atcacgttga gcgtgatctt gccgcgcagc tgcgtgccgc ccttgccata
     4201 cgcccagccg tgctcgagca cgaggtcctg ccactccttg agcagcgcgg gacacgagta
     4261 ccagtagaac gggtgttgga acacgatcac gtcatgtgcg agcaagtctg cctgctcgcg
     4321 cttcacgtcg atcgacagcg ttgggtactc ctcgtagagg tcgcggatcg tgacgccttc
     4381 gatgtcgcgt ataccgtcga caagtcggcg attgacacgc gaacgttcga gcacgggatg
     4441 cgcgaacagg acgagaactc ggcgcatggc tgatttgcgt acgccgcgga cgggtatgtc
     4501 aactgactga cctgccaggg caactgcgag ggaacctgcg cgcgcacgcc ctatgcacgg
     4561 ctcctcgcca tgcagccttc aattgcagaa cggttcttct tcgggcgtcc ctaccatccg
     4621 gtcgccggtg acacagtggt gcccgtcgtc ccacgctcgg gcaccgcgct gctcggtcgg
     4681 attctgatcg ccgcgatctt tctcatcagt ggaatcgcga agctgaccga tcccgccggc
     4741 acgatcggct acatgcatca ggccggcatc tcgcacgcgg acacgctggt ctacgtcgcc
     4801 ggcatcgccg agcttgccgg tggcctcggc atcctgttcg ggttcttgac gcgactggcc
     4861 gcgctcggcc tgttcgtgtt cctcgcgatc gtgcagccct acttccacaa gttctggggc
     4921 ctgcccgcag aggaagcgaa gaacgcgatg gttcagttct tcaaggacct gtcgctcatg
     4981 ggcggcttgc tcatgctcgt cgcgatgggg ccaggccgct acagcctcga cgcgaagatg
     5041 cgccgtaggg ctgcctaggg cgtcgtctcg atgacgagga agtagtcgac ccgtaggtcg
     5101 atacgctcga tgtagagctc gaactgcgta ccagtcgtga gcatcggcga gctggacgtg
     5161 ctgccggtga tgagcgggta gtctgtgccg ccgagctttg gttgaagctt gatctgctgc
     5221 gcggccaccg attcgtcgat ctgcatctgc cacgcgccgg tcggcatggt gccgctgacc
     5281 gggtcaatgc cgagcgagct gtacacgcct tgattcgcgc gactgatctc gatcgatgtc
     5341 ccgccgctac cctcgtagaa ctcgcagtag tggtagatgc taaagccaga accagttcca
     5401 ttgccaaacg cgaacgacac ctggtgcggc ttcgtcgagc caacgctcgc gatcgtgcca
     5461 ccgatgacga cccgcgactc gccgtttccg acggtgagcc gggcctgtgc ctgattcgtg
     5521 gcgttgagcc gcagcgactc acctagcagg ctcgtgttct ggagatagct ccactgcggc
     5581 aacgtcgacg tgaacggatc gaagaacttg atcttctgct tcgcgaccat cggctgtgga
     5641 tcgcaggcgt caccgacgcc atcgccatcg ctgtcgagcg cgttaccggc caagtgcgga
     5701 cacggatcac agacgtcgcc gagtgcgtcc ccgtcctcgt cgtgctggtc gggatttggc
     5761 ttcgcgggac aattgtcgag gttgttcgtg atgccgtccg catcggtatc gccgggcgga
     5821 ccgtcgatcg gcactgcgtc gatcatgacc atcgtgtcgg caggcgcgtc atcgagcgct
     5881 aacggcgcat cggggctggt gtagccaggc tcgcggcact ggtggtcgat gcaaagcagg
     5941 tcgccggggc agaccatgcc gcatgccgag cccacgtcgt acgtcgggct atagcaaccc
     6001 gcgcagagcg cgatggcaac ggcccaccgt agcacccatc aaggataccg gatggctcgc
     6061 ccatctcgcg tggttttgat cgcagttacc cctttaaggc cgccaacgcc ttgaataccg
     6121 cctcgcgggt gcgcgcaaac ccacccgtgc gagccccttc gagcttcttg cgcccactgg
     6181 tacctgcccg tttctcgccg agcatgccga tacccatcgc ggcgttgacc tggacattct
     6241 ccgccgggct ttcgaacgcg tcgcagagga tctcgcatgc gtcaggtagc gcattgacga
     6301 gctcgaggat gcgacgcgct tgtgtctcgt catcggtctc gaggcctttg atcagcgcac
     6361 cgagcacctt cgccttgcgc gcaccaagga ccttgccgga tgccttcgcg acatcggcgt
     6421 caccgcgcaa cgaaccgacc aggaaaccgg ccgcctcgcg caccgcgtcg tcgccgagct
     6481 tgtcgatcga attggccgcg gcgatgcgaa cgcgcatgtc atcgtcgcgc agcaggactg
     6541 cgatgccgct cagcgtgccg aacgccgcgg cgccgaccgc gccgagcgcc gtggctgcgt
     6601 tcgcacgcac gaccgggcgg ccatcgatca gatgttgggc gaggtcgtcg acgcgcagct
     6661 tcgccgggtc ggcaaacgca gcgggctcga gaatgcgctc ctcgaagcct tcgaccgcca
     6721 catcgtcggg cccggtctta cgcgggccgt cgagcatgtc gagcgccttg cgcacggctt
     6781 cacgggtgcg ctcccagccg ccagtgcgcg cgccttcgag cttcttgcgg cccttgccga
     6841 cgcgatcgcg gccgagcatg ccgagaccga gcgccgcgtt gacctgcacg ttgaccgcag
     6901 ggctctcgaa cgcgtcgcac aggatctcca tcgcatcggc caatacgttg atcacctcga
     6961 cgatgcgcgt cgcgtggcgc ggatcgtcgg cctcgagccc gcggacgagc gcgccgatca
     7021 tcttcgtctt gcgcgcgcgc agcacgacag cgacggtctc ggcgactttc tcgtcggagt
     7081 cgcgcagtgc accgaccaga tagtcggcgg tttcggtcac ggccgcatcg ccgatccgat
     7141 cgagtgcttg ggcggcgccg aggcggacct tcgcagcgtc gtcgcgcagc agcacaccga
     7201 gcgccagcgc ggagccttgc gatgcggctc cgagcacgcc gagcccgagc gctgcgttcg
     7261 cgcggacgac gtcgcgacca tcggtgaggt acgcggtgag gtcgccaaca cccgctttct
     7321 cgacgtcggc cttcactttg tccagctcgg cgagcgtcag gaatcgatcc tcgaacccgc
     7381 cgatgctgac ctgcttcggt ccggtgtcgc cgcgcgtctc gatgatgtcg agcgcagcac
     7441 gtacggcggt tcgcgttcgc acgtcgccgc cggtgcgcgc accgtgaagc gcagcgaggc
     7501 ccgccgcggt gatgctcttg ccgaacatgc cgaggccggc tgcagcgttg acctgcacgt
     7561 tcacggccgg gctgttcttg aactgatcgg tcaggatgtc agcggcgcgc ggcagcttca
     7621 cgatcagcgc ggcgacgcgg cgaccgtgct cttcaccacc tgcctcgagg cctttgacaa
     7681 gcgcctcgag cgcgcgctca cccaacgctt cgagcgtatc ggatgctgcc tcggcgactt
     7741 cgccatcgct gtcgccaagc gcgccgacga gatcgggtgc ggctgccacc gcctcgacgc
     7801 caagcttacc gagcgacttt gcggtctcgc gccggaccga cgccacgctg tcacgcatga
     7861 gcaggcccat cgcagttgct gccctgcccg ccttcgcgcc cttcgccgcg agcgcacggc
     7921 ccgcgttgac cttcacgtga tcgcggccgt cctgcagcgc gaagatcatc tgatcgatat
     7981 cgatcacatc ggcgtactcc gacagctcgc tgttcgtcag cttccgagcc tcgaaatcgg
     8041 ggatgttctt cggcagcgaa tcgacggcgg cgaccttctc gcgcgcgacg acggccagca
     8101 tcgcttgctt cgccgcggtg cggacgtcgg gaaccggatc gctcgcctcg atctgcgtca
     8161 gacacgccat cgcggtgtcg gcgtcggtct tgccgagctt gccgaggccg gcgatcgcat
     8221 tgacgcgaat gcggctgcgc tcgtgccgag tcgcgcgcgt caagaatgca atgccggcct
     8281 tgccgacgac cttgcacgct tcgagcacgc tcttaatcgc gttgtcgagt ggcacgtcga
     8341 ggccgagtgt catctcctcg tcggctttgc caaccaggtc ggcaagtgcg gcgacgatcg
     8401 cctcggtgac ctcgggcttc atgccctcgg ccgcctgtgt gatgcccggg atcaatggac
     8461 gtgcgagcgg accgagccgc gtgatcgctt cggcgccggc gaggccgacg ctgatctcgc
     8521 tatcgcgaag cagatggacg agctcgctcg cgggatgatt cgcagcggcg agtgcgagcg
     8581 cggcattcgc gcggacgatc gcacgaccat cggcgaggtg cgcgacgagc tcggccggca
     8641 agaatgtgcc gccgtacggg atcagctcgg cgtacttgag gcgacgctcg tcgagtggcg
     8701 gtggcggccc gtcggcacct ttcactttct tctccggctt cgcctccgct ttcgcgtcct
     8761 ctttggccgc cggcttggcc ttgtcgtcgg gcttcccctt ctccgattcg ttgctcacgg
     8821 ggcccatcct agccctagtc ggagatcaac cgtagtgata ggcgctcaga cgtagcccgc
     8881 cgaacgtacc gttgaatgcg accgtgccgc gatcgttgcc ggcggcgcct gcaatcacca
     8941 tgagcgggat cagatgctcc tcgcgcggat gcgattcacg cgcggccgga gcacgcgacc
     9001 aatcgatcag cgcgcgatcg cgttcggcct gctcgcgggt cgcggcgtcg cgcagccacg
     9061 cgtcgaacgt ctcggcgatc ggtttcgcgc gcggatcgcg aaacacgcgc aggttgtgga
     9121 acgacagccc gctgccgatg atgaacacac cctcgtcgcg caacggtgcg agcgcgcggc
     9181 cgatcacgat gtgctcttcc ggatcgagcc ccgccttcag cgagagctga acggttggaa
     9241 cgttcgcgtc cggataggtg agcttgagcg ggatgaacgt gccgtggtcg aagccgcgct
     9301 tcgcgtcggt cgcgctcgga atgcccgcct tcgcgagcag ctcttgcacc cgcgccgcga
     9361 gcgccggctc accgggcgca ggccactgga tctcgtacga cgcgggaggg aacccgtaat
     9421 agtcgtagag catcggcggc tgcggcgacg tcatcaccgt cggcacgcgc tcctcccagt
     9481 gcgccgagat cacgagcagt gctttcggcg gcgtcttcgg cagcgccgcg acgctgcgca
     9541 gatatgcggc gagctcgtcg gtatccgtca gattccatcc catgtcgaca aacggccacg
     9601 gacctccgcc gtgcggcaga tacgcgaccg gcatgcgtgt attgaccgtc atgtcgtccc
     9661 atggtggtta ccgcgccgac aggccgcaac gctagcagtc aagacggact gtctcaccgc
     9721 gcgccggcgg caggcacacc gctgttacta acgcgccgct gtgtaatcgc cagcgttgcc
     9781 tcggccttga gcgcgagggc gtcgcgaatc cgcgccacac gagacggcgg atgctggaag
     9841 aaattgcggt cgagcgcagg atcggtcgcg tacgcgcggc ggatctcggc ctcgtcccag
     9901 cgcgtcggaa acgcgggata tgcgagtggc gcttcgcgga tccaccaacc ggtgaagccg
     9961 aaccgcgcgt ggttctggtc gtagatctgc gtgccgggat acggcacgac gacacctcgc
    10021 gcgttgaagc caccggcgag cggcgccgca cgatcgagaa agccgatcgt cgcatcgagc
    10081 tcggcgtcgg tctcgtcggg ccagccgaac atgaggttga cgaccgtatg gatctcgagc
    10141 tccttgcacc acgcgagcac gtcgagcacg cgctcgaccg tgacgccttt gccgatgcgc
    10201 acgagcatgc cggggtcggc gctctccatg ccgatgtcga tgcctgaaca acccgcgcgc
    10261 ttcatgtcag cgagcacgtc gcgatcgaga tgcgcgggat gcgccgtgca cgtccaccac
    10321 acctgcgtac cgagagtcgt gatcgcatcg cacagctcgc ggacgcggcg gcgaccgacc
    10381 gcgaacgaat cgtcgaagaa cgagaacccg atcaagccgc gcccgcgaag caacgcgacc
    10441 tcttcgacaa ccgagcgcgc gctgcgatag cgaaacttgc ggcccgtaac gtcgttcgag
    10501 cagaacgtgc acgcggccgg gcagccgcga ctcgacagga tgccggcggg cgcgagtgcg
    10561 gtgccgggca gcgccggata ccagcgcgga tcgaacagat cgagtgccga cagcggtggt
    10621 gcgaggcgat cgagatcggt gttgaacggt cgcgccggat tgtggcggac catgccgcga
    10681 tcgatccacg acaggccgga gatgtcgtcg atcgaacgca tgtcgcgacc ggtgtcgcgc
    10741 cggcgcatcg tatcgatcgc atcggcgagc tcgacgagcg tatcctcgcc ttcgccgcga
    10801 atcgcgtagc ggaaaccgcg cgcgagcggc tcgtcaggaa cgatcgtcgc gtgcggacca
    10861 ccggcgacga gcgtgaaccc ggcgagtgcc tcggcaagcg cgtaggccgg ctgcacgtgc
    10921 aaggtcttca ggtgaatgcc gacgagatcg ggcgcgaatg ccgcgacgaa cagatgaatg
    10981 tcttcgtgcg aatgcggcgc gttcggtgcg gacgcatcga gcaagccgac atcatgcccg
    11041 gcagcgcgaa gcgaagccgc gaggtggcag aacgagagca gcggcgtgtg tactggttcc
    11101 ggtgacggcg gattcaccag aaagacgcgc gccatcggcc tagcgtatca ccagcgcgaa
    11161 gccggctcgc tcgcagtttt tcgccgcctc gcaaatcgcc gtcgcagcct cgggaatcgc
    11221 gccgaggtcc gagagctcga gcgagtacgc ggcgatgccg ttcgcgacga ggcgcgccgc
    11281 cactgctggt ggcaaccagt acgacggcgg caacgggtcg acggcgctct ggtacgcggt
    11341 cgtcaccgtt cgcgcgaccg catcgatcgt gatcgtgccc ggcatgcgac tacgcgtgcg
    11401 aaacgagcca gcgacttgca ggttgtcgag gcacatcgcg cgcagcgcat ccttgacgat
    11461 cctgcgatcg aacgcggggt catcgccggt cgtcgcgtgg cggagcgcga gctcgaccca
    11521 cggccccggc gcgaacgtgt cggataggaa gtgaagcgta cggcgcgcgc gccagtcgtc
    11581 ggctgcctgc cgcatcgccg cgtcgagaaa tgcggatggc aacaccgcgg accatagcga
    11641 gtggccggta tgaccgcgaa cgactgcgcc aaggccgaac gcacgcacca ccgcctgtcc
    11701 gacctgcgcc agccacggcg atgcgtagat cgcgtcgtcg tgcccggact gcggatacgc
    11761 gaattcgggc cacacgcgca cgccgagata gccggcgacg cactcgtcga gatgcatcgg
    11821 cagcgcgtcg cggtagcggc cgaagtgaca cagctcgtgg acgagcggcg cggtgaggta
    11881 gcgatcgatc gtcgcgtgga gctcgaggcc gcgcgacatg aagtacgcga ggtcggcggg
    11941 atagccgtag agcagcggca tgcacgcgcc gtaatgcgtg cgcatgtaga gctcgccgaa
    12001 cgtcggtgcg ctcgcgcgca attcggctgc gctcgcggcg ccgagtggca gctccgcatc
    12061 gacgccgcac tcgacaagca gcgcgaacag gcgatcgagc gccgccgaca tgcgcggtag
    12121 ccgcgcccaa tcgagcggag aatgcatgag cgtcgcggca ggctcgtccg ttccgcagat
    12181 ctgcgtgacg cgcatgcgcg gcatgatctc ggtgtagtgg cgccgaatgt ctgcgttgtc
    12241 tgcgatgtcg tcgctgtcgt cagcgaccgc acgtgcgagc acgcggcgca acggcgccgg
    12301 atacgcgagc cgcctcggat gcgcatcggc acgtaaccgg ccctccgcct cgaggtcgcg
    12361 ccacgcgatc ttgcctatgc gtgacagctc gctatcgagc tcgcgcgcgg tcgcgtcctc
    12421 tgcgggcttg cggccgatca cgacgatcgc gtcgggtgag gtcgcgagac ggcgttcctc
    12481 gacgtcgacg agcccggcgg cgatgagcca gctgcgaatg cgcgagctct cgtagacgtc
    12541 accgcccggc gtgtacagcg ccatgttgag cgcgaacacc agcccctcac gcggtcccgc
    12601 cctaccctcg tccatccgca gatccttgac gacgacgacg ccgccgggcg cgatcgcctt
    12661 cgccgcgagc tcgcacagcg cggcgccgat cgactcgtcg tgcaggtgca gcacgtttgc
    12721 gagcaacgcg acgtcgtagc tctcgccaag ctgcaccgcc cgcatgtcac ccgcgatgaa
    12781 ggcagcacgg tcgccgaacg gcgcgagcgt gtcgcgtgcg agcgccacga cgtccggcac
    12841 atcgacgagc gtcgccgtcg cgtcggaccg cgccgcgagc cacgcacgcg tgtatgcacc
    12901 cgcgccgccg ccgagatcga gcaaccgccg gagcggcgcg aggccagcga taatcgtgac
    12961 gagctcggct gccgcagggg cacctgcact gacgaggtgc tcgtgcatcc gctgggtcgt
    13021 gacggtatcg ccgagcgcga gtgggttgtc gctgcggatc acattcgcga gcacgcccca
    13081 accagcgggc ggcacgaccg gccgctcggg cacgtgagcc gccgcgtagc gatcgcggat
    13141 tcgcgcgagc acgtcgaacg aaacgagcac gtcgagcaac ctgcgtagcc gccgcgcacc
    13201 aacgccgatc tcagtcgcaa cgtcgccgac cgtgcgcggc tgaccgaggg ctgcaaacac
    13261 gccgagctcg tgcgcggcag caacagcggc gtcgcgaaaa aagtcgcccc cggcgctgag
    13321 cgctccgccg aagacgtcca ctacttgtcg gggttcaaat ggatcatcgc gccgatcacg
    13381 acgacgtcgc ccttcgcctc gacgtcgatc ttctcggtcg ctttgatcgc gacctgcttc
    13441 gactcgatct cgacggcctc ggtcgccttg agcgagagct tcgcgctctc catctgtaag
    13501 accgggcctt gctcggtgag ccggatccgc acctcgagca tgcccgattc gttacgaatc
    13561 tcgacgagct gctcgccacc ctgctcgctg acgaccagct tgcggccgtc gcgtagatag
    13621 acctcgcgct cgtcgctgac ctcctcagct ccactaagag tctggagttt ttccttcgga
    13681 gatccggaca tgaatggagg ctatcacata cgatgcaatc ggccgcatga cgctggatct
    13741 gctggtctgt cctggttgtc gaacgctcag cggcgatcgc ttggacgtgt cgacgctcga
    13801 tcccgccggc gacgtgctgg cgtgcgagtg cggccgccgc tatccgatcg tggatgacgt
    13861 tccgatcgtg atgacggacg ccaccacgta cctgcgcaac gaagtggcga ccatcgtcga
    13921 gcgcgacatg gctcccgagg ttgcggcgct gctcgtcgca cccggtcccg acgaggcgcc
    13981 ctacccacgc atgctcgagc acctctcgat ctatctcgac gcgcattggg gcgatcgcgc
    14041 cacgccaccg cctggctttg cactcgcgcc gttcgtcgag cgcatcggcg cgctgccgaa
    14101 agtctcgatg gcggtcgagc tcggctgcag cgtcggccga gtgctcgcgg agctgcctgc
    14161 cgaacgtgcg gtcggtctcg acatgcactt cggtgcggtt cgccgcgcgc gtcgcctgct
    14221 ctccggcgag tcactcgcat acgcgcgccg cgaggtcggc cggcactatt cgaccgcgac
    14281 cgcggccggc aaacgcaccg ccgatcgaaa gctcgtgtgc gccgacgtcc tcgacccgcc
    14341 gctcttgccc gatgtctggg accgcgtcgt cgcgctcaac atgctcgact cggtcgcgca
    14401 cccgcgtcag ctgctctcgg tgatcgatgg cctctgcacg ctcggcggcg agatcatcgt
    14461 gtcgtctccg tacgcctggc agagcgcggt gctcgccgac cacgagcggt tcgggggcgc
    14521 cgatcccgcg accgccctcc gtactctact gacggaaggg accgggcttc gcgcccgcta
    14581 ccgcatcgag gacgaggccg aaattttgtg gacgttgcgc agggatgcgc gtagcgcggt
    14641 cacctatcgc acccactaca tccgcgctcg caagggaacg taaaccggcc accttccgta
    14701 tactctttgc ccacagcacc atgcccgctc cgcaagcagc cgcgatgcag caactggccc
    14761 ggttgaagtt cacgagcttc aacttgaagg tgccgaagga ctggcgcgat ccgtcaggcg
    14821 gcgatgccgg cgatcactac tcgcgcgcgt tcaagcccga cgaaaaagcc tcgacacccg
    14881 gcatgccgcc gctgctacag cccgcgtcgc tgaacaagta ccacaccgat actcagaaga
    14941 tgcacatcgc caaggtcggc gcgttcatcg acggcacgtg ctcggcgatc tgctcggcgt
    15001 ggagccagtg gcagaccgcc gcaacgatgg tcggtgtcgt gatcacggcg atcaccgcat
    15061 cggtcggtca ggtcgtcggc ccgccgtgga cgccgctcat catggcgagc gccccgaagg
    15121 catcgccgat ggagctcaag tactcgaacg tgatcgcgac ggtgctcggc accgcgtggc
    15181 tgcagtacac cgcgacgatc aagatt
//
LOCUS       DASUFX010000472         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1604881, whole genome shotgun sequence.
ACCESSION   DASUFX010000472 DASUFX010000000
VERSION     DASUFX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604881"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VFV99_18825"
     CDS             complement(<1..1233)
                     /locus_tag="VFV99_18825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5061432.1"
                     /translation="MRPLLSYEIRIGWRYLYGGKRDKLMLLCAGVSLLVTLGGLAWVL
                     TSGGTSPYGVLTLVLGMISTAVFGLLSLFTVFTSVSVLGVALGVAALTIVLAVTTGFQ
                     QQFRDKVLGVNAHVIVLKSQATFAEYRDVMTTAKRIDPDVLAVQPFIFAEMLVTRGKG
                     ELSGVAIKGVDPKLVRGVLDLDQHMIEGSIDTLAKEPGPGELPPIIIGKELAHKLGKA
                     KVGDDVTVVVPLSNIDFDTWRAKSSAPRTRKFRVTGIFYSGFDEYDRRLMYTALRDTQ
                     ELVGRGDQVMGVELKVKNVDRAAEIAKRLEDALGGPPYQVQDWYALNHNLFTALNLQK
                     LALVIILTLIIIVAAVNMISALTMMVTDKTREIAILKSMGATSSSVSRVFQFLGIAIG
                     GVGTIIGVAIGLVTCYVVS"
     gene            complement(1307..1825)
                     /locus_tag="VFV99_18830"
     CDS             complement(1307..1825)
                     /locus_tag="VFV99_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061433.1"
                     /translation="MLAPAKKPVDVLRSMAAPRELVEWVRKMNPDEAVRRAWIDVTRA
                     DWMPYLAVLRGISKDTIVRATCACARELGEPVLASPEGTRILAALRDESLASAEQDLD
                     DLRLVMLQHGEANQLPWMFWCRLVLELARATRRGNPLIGVALALRMLASTGGRRAQSD
                     LVARFRDTLVLA"
     gene            1915..>2102
                     /locus_tag="VFV99_18835"
     CDS             1915..>2102
                     /locus_tag="VFV99_18835"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5061434.1"
                     /translation="MPEAARVPRTQQQRRDETRRALLDAAVESLIEVGFARTTTLEVQ
                     RRANASRGALLHHFPSKT"
BASE COUNT          376 a          707 c          676 g          343 t
ORIGIN      
        1 gctgaccacg taacaagtga cgaggccgat cgcgacaccg atgatcgtgc cgacgccgcc
       61 gatcgcgatg ccgaggaact ggaacacgcg cgacacgcta gaagacgtcg cgcccatcga
      121 cttcaagatc gcgatctcgc gggtcttgtc ggtcaccatc atggtgagcg ccgagatcat
      181 gttgaccgcg gcgacgatga tgatgagcgt caggatgatc acgagcgcga gcttctggag
      241 gttcagcgcg gtgaacaggt tgtggttgag ggcgtaccag tcctggacct gatagggcgg
      301 cccgccgagc gcgtcctcga ggcgcttcgc gatctccgcg gcgcggtcga cgttcttcac
      361 cttgagctcg acgcccatca cctgatcgcc gcgcccgacg agctcctggg tgtcgcgcag
      421 cgcggtgtac atgagccggc ggtcgtactc gtcgaagccg ctgtagaaga tgccggtgac
      481 gcggaacttg cgcgtgcgcg gcgcgctcga cttcgcgcgc caggtgtcga aatcgatgtt
      541 cgacagtggg accacgacgg tgacgtcgtc gccgactttg gccttcccga gcttatgggc
      601 gagctcctta ccgatgatga tcggcggcag ctcgccgggc ccgggctcct tcgcgagcgt
      661 gtcgatcgag ccttcgatca tatgctgatc gagatcgagc actccgcgca cgagcttcgg
      721 atcgacgccc ttgatcgcga cgccggacag ctcgcccttg ccgcgagtga cgagcatttc
      781 ggcgaagatg aacggctgca ccgcgagcac atcgggatcg atgcgcttgg ccgtcgtcat
      841 cacgtcgcgg tactcggcaa atgtcgcttg cgacttgagc acgatgacgt gcgcgttgac
      901 gccgagcacc ttgtcgcgga actgctgctg gaagccggtc gtgaccgcga gcacgatcgt
      961 gagcgcggcg acgccgagcg cgacaccgag caccgacacg ctcgtgaaca cggtgaacag
     1021 cgacaacaag ccgaacaccg ccgtcgagat catgccgagc acgagcgtca gcacgccgta
     1081 cgggctcgtc ccgccgctcg tcagcaccca ggcgaggccg ccgagcgtga ccaacagcga
     1141 cacgccggcg cacaagagca tcagcttgtc gcgcttaccg ccgtacagat atcgccaacc
     1201 gatgcgaatt tcgtagctca agagtggcct cactttgcct cggcggagcg cctcgcgcca
     1261 gccaatcggc ccggagacac cacgcgaagc cggcaagttg cgtccgtcag gccagcacga
     1321 gcgtgtcgcg aaaccgcgct acgagatcac tctgtgcgcg ccggccgccg gtgcttgcga
     1381 gcatccgcag cgcgagcgcg acgccaatca gtggatttcc gcggcgcgtg gcgcgtgcta
     1441 gctcgaggac gagccggcac cagaacatcc acggcagctg gttcgcttcc ccgtgctgca
     1501 gcatcacgag ccgtagatcg tcgagatctt gctcggccga tgcgagcgac tcgtcacgca
     1561 aggcggcaag gatgcgcgtg ccttcgggtg aggccagtac gggctcgccg agctcgcgcg
     1621 cgcacgcgca ggttgcacgg acgatcgtgt ccttcgagat cccgcgcagc accgcgagat
     1681 acggcatcca gtccgcgcgg gtgacgtcga tccacgcgcg ccgcactgcc tcgtcgggat
     1741 tcatcttgcg gacccactcg acgagctcgc gcggcgccgc catcgaccgc agcacatcga
     1801 ctggtttctt ggcgggcgcg agcatccttc caactcccga cgttactctg gtttcgcgat
     1861 cttagataca aacagtcttg actgactgtt tcttgggcgg tacaccctcc tcgaatgccg
     1921 gaggccgccc gcgtaccgag gacgcagcag caacggcgcg acgagacccg ccgcgccctc
     1981 ctcgacgccg ccgtcgagag cttgatcgaa gtggggttcg cgcggacgac gacgctcgag
     2041 gtccagcggc gcgccaatgc ctcgcgcggc gccctgctcc accacttccc atcgaagacc
     2101 ga
//
LOCUS       DASUFX010000473         8023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2021369, whole genome shotgun sequence.
ACCESSION   DASUFX010000473 DASUFX010000000
VERSION     DASUFX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021369"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VFV99_18840"
     CDS             <1..1397
                     /locus_tag="VFV99_18840"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HEX5061435.1"
                     /translation="LDRFANAPIDDDVRTRVIELLRDQPDEAVTQIHPLEMAAAWQLD
                     GRKTIEAFLHATRSGLTELEWQIDCPSCRVGTDAVPRLDAIRGRIHCAECNISFDVDF
                     AANVHATFTVHPAIRKVARVVYCATSPYFSPHVHGYVTLAPDEGRELGPLPAGGILLR
                     ARGTTRSLTIDRETAGAAIAIGDEAITVDDTAAPGEHTLRVVNRGSHPARLQVERAGW
                     RSLRAHGGLICTIPGFTELFGTDAPATGLPLAIGRLAVLFTDLVGSVDLYNKVGDARA
                     FALVQEHWRDAMAAIAERNGAVIKTLGDGVFASFTELSDAVEAAIDIMAAGERLGRDH
                     QIPFAIRAGIHEGPCFLVRANDRLDLFGSTVNLAARLSATAGGQQLAMLETSANQVSR
                     VFERERCEVEHVMSRIRGLPGEFRIGMMTRSGRGLATGERPLIERSRTGPILARSSTP
                     ELPIVPSKDPASVD"
     gene            1421..2749
                     /locus_tag="VFV99_18845"
     CDS             1421..2749
                     /locus_tag="VFV99_18845"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061436.1"
                     /translation="MVNTRTLSIDESVHAQMQRVDHLYVVIAGGKPGGGARWSLTNID
                     KIEIGRGGQRAIKRDGGTLRVEVPDPWMSSNHLRFERDRAHWLAVDAGSRNGILVNGA
                     KQDRAIVADHDVIEAGRSFFMLRVSEAAGEAALELPAKPQAGGLVTLDPALARAFEEL
                     ARIAASQVPVLVGGPTGSGKERVAFGIHTMSRRKGAFVPVNCGALPSGLVESELFGHK
                     KGAFSGAIADAPGLVAASSNGTLFLDEIGDLPAPAQAALLRVLEEHEVRAVGATAPVA
                     VDLRVVAATHRDLDAMIEDGNFREDLLARLAGFELELPALADRRVDLGAMLAEIAPDA
                     TFAAAAVRALLAYAWPRNVRELVRAVERAAALAAGGEIGAAHLPEEIASAKFTAPAVA
                     PADARRDELVALLEKHHGNVSKVAADLGRVRQQVQRWLKRYGLDPERYRS"
     gene            complement(2827..3294)
                     /locus_tag="VFV99_18850"
     CDS             complement(2827..3294)
                     /locus_tag="VFV99_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061437.1"
                     /translation="MKCLLAAALALVACDKLPERPDETKFKAMDAWEKCRATASRAIM
                     CTNELMVAEMRSIAAFDGSDDFTAELEKQMANDPKLPKQERKANIDIHKTTCRGDRET
                     GYVDGIFRCWAIEDCKKFATCVYERPTRAPMPTPTPSAPPTPTPSAPPTPTEP"
     gene            complement(3291..3845)
                     /locus_tag="VFV99_18855"
     CDS             complement(3291..3845)
                     /locus_tag="VFV99_18855"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5061438.1"
                     /translation="MNALLSGDEVRAPAPGWFHLLVAADHLVTQGDAIGELEILGRTV
                     RIIAPAVRGIVKLPPGPALARRAVSYGDLLFTVATDVAIHGGADLAATAAAQAAQGLV
                     FRAPTSGRFYGRPTPDKPPFVQAGVELSAGATVCLLEVMKTFHRVTYGGGDVPARARV
                     KDVLVADGADVNQGDALLALEPLA"
     gene            complement(3889..5619)
                     /locus_tag="VFV99_18860"
     CDS             complement(3889..5619)
                     /locus_tag="VFV99_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5061439.1"
                     /translation="MAFVPLVPIGDSLGKLVDERTAADQRTALAVLEDRLRTRRAEVH
                     AGWGPTYVDRVHKKGKLTSRERIARLIDPGTRTFEVGTFVNYGEVFPGDQKSPAAGVV
                     TAFARIEGRWCMVIANDNTVASGAWWPRTPEKIQRAQTMALRLRIPTIYLVDCSGLFL
                     PEQSKSFPGARGAGHIFKMNSLLSANGVPQIAGVFGDCIAGGGYMPIISDRVYMTEQA
                     YMVIAGAALIKGAKSQKITSLDIGGPEIHVHQSGCADVRVPDDETLLACLRRDVARLP
                     SSGADFYRGDLGIVDPRFAPAELAAMVPADHREAYDANQVLARLVDQSLFWEVMPEVG
                     EEMICGIGRVAGLYAGFVINRQGLVGDPEHPGRQRPAGILYRGGIAKISAFSRACNDD
                     GIPLIWLQDISGFDIGHEAEAHGLLAYGSSLIYTNSTNTTPMFTVLLRKASGAGYYAM
                     SGLPYDPVVQLSTSYARLSVMEGRTLAIAAFNTKLDDDFQIMATDPAERAKIQAGMAD
                     TEARIERDMDPFVAARQMDTDEIIELGELRAYLTALVEAAYQNTGYRRVKNPRIWSMH
                     DLRVLVGGPR"
     gene            complement(5676..6554)
                     /locus_tag="VFV99_18865"
     CDS             complement(5676..6554)
                     /locus_tag="VFV99_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061440.1"
                     /translation="MRLLVGLAVVVGCSTPGAQRVPAAALPYDPGFVASSQRLRATLA
                     RDNIELDTQPVITTCDPGDTRKNCARCDVATVLDQIEPELVDQMAIAFARYPSSVRKT
                     ARLEHVAFCRAITYVRGTDHGPAGLADPNAHRVYISVEYFRGAGSNLSIESAVHHEVF
                     HLLDYETLHREEDPAWAALNPKGFAYVDPSVGWERPKGFVNTYATTNQMEDRASTWEY
                     LMVQPDDLCDMAAKDPVVAKKVRAIWQRVVKVEGANRLGVSARCALKAEQPTKPTKPT
                     TRRTPKKPNLQLPTDW"
     gene            complement(6732..7040)
                     /locus_tag="VFV99_18870"
     CDS             complement(6732..7040)
                     /locus_tag="VFV99_18870"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5061441.1"
                     /translation="MSDDFKTLDSEQLLDINGGLDMPASARWIMQHESSGNPRAQNAH
                     STAFGAFQMLKATRKQYMGADWQSTDLAKQYAGASKYVNQRYGGWDNAKAFWQRHHWY
                     "
     gene            complement(7275..>8023)
                     /locus_tag="VFV99_18875"
     CDS             complement(7275..>8023)
                     /locus_tag="VFV99_18875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827241.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5061442.1"
                     /translation="FLEKLIEGGRHVEIQIFADRHGHAVHLGERDCTVQRNHQKLIEE
                     SPSPALTADQRGRVAEAAARAAARIGYVGAGTMEMLLDPGGTLRFMEMNCRLQVEHTV
                     SEERTGKDLVIAQLEIAAGRPLTWKQDAIPLTGHVIECRINAEDPANNFAPAPGKITA
                     WQPPAGDGIRVDTHVETGYVVPPFYDSLLAKLIVRGENRDEAIAKMIGALSAFVVEGV
                     PTTIPMHLAILKSEAFRTGAYDTRTIPGWP"
BASE COUNT         1238 a         2641 c         2789 g         1355 t
ORIGIN      
        1 agctcgaccg cttcgccaac gcgccgatcg atgacgacgt gcgcacgcgc gtgatcgagc
       61 tgttgcgcga ccagcccgac gaggcggtca cgcagatcca tccgctcgag atggcggcgg
      121 cgtggcagct cgacggacgc aagacgatcg aagcgttctt gcacgcgacc cgcagtggcc
      181 tcaccgagct cgagtggcag atcgattgcc cgagctgccg cgtcggcacg gatgcggtgc
      241 cgcgcctcga cgcgatccgc ggccgcatcc actgcgccga gtgcaacatc tcgttcgacg
      301 tcgacttcgc ggcaaacgtg cacgcgacgt tcacggtgca tcctgcgatt cgcaaggtcg
      361 cgcgcgtcgt gtactgcgcg acgtcgccgt acttcagccc gcatgtgcat ggctacgtga
      421 cgctcgcgcc ggacgagggt cgcgagctcg ggccgttgcc agcgggcggc atcttgctgc
      481 gtgcgcgtgg cacgacgcgc tcgctgacga tcgaccgaga gacagcaggc gcagcgatcg
      541 cgatcggcga cgaggcgatc accgtcgacg acaccgcagc gcccggcgag cacacgctac
      601 gcgtcgtcaa tcgcggttcg cacccggcgc gcctgcaggt cgagcgcgct ggctggcgct
      661 cgctgcgcgc gcatggcggc ctgatctgca cgattcccgg cttcaccgag ctattcggca
      721 ccgatgcgcc ggccaccggg ttgccgctcg cgatcggccg gctcgccgtc ctgttcacgg
      781 acctcgtcgg ctcggtcgac ctctacaaca aggtcggcga cgcccgcgcg ttcgcgctcg
      841 ttcaggaaca ctggcgcgac gcgatggccg cgatcgcgga gcgcaacggc gccgtgatca
      901 agacgctcgg cgacggcgta ttcgcgtcgt tcaccgagct gtcggatgct gtcgaggccg
      961 cgatcgacat catggcggct ggcgagcgac tcgggcgcga tcatcagatt ccattcgcga
     1021 ttcgcgccgg catccacgag ggcccgtgct tcctcgtccg cgcgaatgat cggctcgacc
     1081 tgttcggctc gacggtgaac ctcgctgcgc gcctgtcggc gaccgccggc ggtcaacagc
     1141 tcgcgatgct cgagacgtcg gcgaaccagg tcagccgcgt gttcgagcgc gagcgctgcg
     1201 aagtcgagca cgtcatgagc cggatccgcg gcctgcccgg agaattccgc atcgggatga
     1261 tgacgcggtc gggacgcggc ctcgccaccg gtgaacggcc gctgatcgag cgctcgcgca
     1321 cgggaccgat cctggcgcgg agtagcacgc ccgagctgcc gatcgtcccg tccaaagacc
     1381 cagccagcgt cgactaacgg ctaagattgc gccgagggga atggtcaaca cgcgcacgct
     1441 ctcgatcgac gaatcggtcc acgcgcagat gcagcgggtc gatcacctgt acgtcgtgat
     1501 cgccggcggc aaacccggcg gcggcgcgcg gtggtcgctg accaacatcg acaagatcga
     1561 gatcgggcgc ggcggtcagc gcgcgatcaa acgcgacggc ggcacgctcc gcgtcgaggt
     1621 gcccgatcca tggatgtcgt cgaaccacct gcggttcgag cgcgaccgcg cgcactggct
     1681 cgcggtcgat gcgggctcgc gcaacggcat cctcgtcaat ggcgcgaagc aagatcgcgc
     1741 gatcgtcgcc gaccacgacg tcatcgaagc aggccgcagc ttcttcatgc tgcgcgtctc
     1801 cgaagcggcg ggcgaagccg cgctcgagct ccctgcgaag ccgcaagccg gtggcctcgt
     1861 cacgctcgat ccggcgctcg cacgcgcatt cgaggagctc gcacgcatcg ccgcctcgca
     1921 agtgccggtg ctcgtcggcg ggccaaccgg cagcggcaag gagcgcgtcg cgttcggcat
     1981 ccacacgatg tcgcggcgca agggcgcgtt cgtgccggta aactgcggcg cgttgccatc
     2041 agggctcgtc gagagcgagc tgttcggtca caagaaaggc gcgttctcgg gcgcgatcgc
     2101 cgatgcgccc ggcctcgtcg cagcatcgag taacggcacg ctgtttctcg acgagatcgg
     2161 tgatctgccg gcacccgcgc aagccgccct gttgcgcgtg ctcgaagagc acgaggtgcg
     2221 cgcggtcggt gcgaccgcac ccgtcgcggt cgatctccgc gtcgtcgcgg cgacgcatcg
     2281 cgatctcgac gcgatgatcg aggacggtaa cttccgcgaa gacttgctcg cgcgactcgc
     2341 cggcttcgag ctcgagctgc ccgcactcgc cgatcgccgc gtcgacctcg gcgcgatgct
     2401 cgccgagatc gcccccgacg ccacgttcgc tgccgccgcc gtccgcgcgc tgctcgcgta
     2461 cgcgtggccg cgcaacgtgc gcgagctcgt acgtgcggtc gagcgagccg ccgccctcgc
     2521 tgccggtggc gagattggcg cagcgcatct ccccgaagag atcgcgagcg cgaagtttac
     2581 ggcgcctgcc gtcgccccgg ccgacgcacg ccgcgacgag ctcgtcgcgt tgctcgagaa
     2641 gcatcacggc aacgtcagca aggtcgcagc tgatctcggc cgcgtgcgcc aacaagtcca
     2701 gcgctggctc aagcgctatg gtctcgatcc cgaacgctat cgatcataga cgacgaccct
     2761 caacctggtt cgcgatcgta atgcatcgaa agctcgcgaa tgcgccgtgc gggggcgcgt
     2821 ggccggctag ggctccgtcg gagtcggcgg tgccgagggc gtcggagtcg gcggtgccga
     2881 gggcgtcggc gtcggcatcg gtgctcttgt cggccgctcg tagacgcaag tcgcaaactt
     2941 cttgcagtcc tcgatcgccc agcagcgaaa gatgccgtcg acatacccgg tctcgcggtc
     3001 gccgcggcac gtcgtcttgt ggatatcgat gttcgccttg cgctcctgct tcggcagctt
     3061 cgggtcgttt gccatctgct tttcgagctc ggccgtgaag tcgtccgaac cgtcgaatgc
     3121 cgcgatcgag cgcatctccg cgaccatcag ctcgttcgtg cacatgatcg cgcgcgatgc
     3181 ggttgcgcgg cacttctccc acgcgtccat cgccttgaac ttcgtctcgt cgggacgctc
     3241 cggcagcttg tcgcacgcga cgagcgcgag cgcggcggcg agcaggcact tcacgcgagt
     3301 ggctcaagcg cgagcaacgc atcgccttgg ttgacgtcgg caccgtcggc gacgagcaca
     3361 tccttgacgc gcgcacgcgc tgggacgtcg ccgccgccat atgtgacgcg atggaacgtc
     3421 ttcatcactt cgagcaggca caccgtcgcg ccggctgaga gctcgacgcc cgcctggacg
     3481 aacggcggct tgtcgggcgt cgggcggcca tagaagcgac cgctcgtcgg cgcacggaac
     3541 acgaggcctt gagcggcttg cgctgcggcg gttgcggcga ggtcggcacc gccgtggatc
     3601 gccacgtcgg tcgcgacggt gaacaagagg tcgccgtagc tgacggcgcg gcgcgcgagc
     3661 gcgggcccgg gcggcagctt gacgatgccg cgcacggcgg gcgcgatgat gcggaccgtg
     3721 cggccgagga tttcgagctc gccgatcgcg tccccttgcg tgacgaggtg gtcagcggcg
     3781 acgaggaggt ggaaccagcc tggcgcggga gctcggacct cgtcacctga gagcagggcg
     3841 ttcatcgcga acctcccacg agcggagtaa tcgaggagac gagggcgttc atcgcggacc
     3901 tccgacgagc acgcggagat cgtgcatcga ccagatgcgc gggttcttga cccgtcggta
     3961 gccggtgttc tgataggccg cttcgacgag cgcggtgagg taggcgcgca gctcgccgag
     4021 ctcgatgatc tcgtcggtgt ccatctggcg cgcggcgacg aacggatcca tgtcgcgctc
     4081 gatgcgcgct tcggtgtcag ccatgccggc ttggatcttg gcgcgctcgg cgggatcggt
     4141 ggccatgatc tggaaatcgt cgtcgagctt cgtgttgaac gcggcgatcg cgagcgtgcg
     4201 gccttccatg accgagaggc gcgcgtagct cgtcgagagc tggacaaccg ggtcgtaggg
     4261 taggccgctc atcgcgtagt agccggcacc ggaggccttg cggagtagga ccgtgaacat
     4321 cggcgtcgtg ttcgtcgagt tcgtgtagat gagcgagctg ccgtaggcga gcaggccgtg
     4381 cgcttcggct tcgtggccga tgtcgaagcc ggagatgtct tgcagccaga tcagcgggat
     4441 gccgtcgtcg ttgcacgcgc gcgagaacgc gctgatcttt gcgatgccgc cgcgatagag
     4501 gatgccggcg gggcgctggc ggccgggatg ctcgggatcg ccgacgagac cttggcgatt
     4561 gatcacgaag ccggcgtaga ggccggcgac gcggccgatg ccgcagatca tttcttcgcc
     4621 gacctcgggc atgacttccc agaacagcga ttgatcgacg aggcgcgcga gtacctggtt
     4681 cgcgtcgtag gcctcgcggt gatcggcggg gaccatggcc gcgagctcgg cgggtgcgaa
     4741 gcgcggatcg acgatgccga gatcgccgcg atagaaatca gcgccgctcg acggcaggcg
     4801 cgcgacatcg cggcgcaggc acgcgagcag cgtttcgtcg tcgggaacgc ggacgtcggc
     4861 gcagccggat tggtggacgt ggatttcagg gccaccgata tcgagcgacg tgatcttctg
     4921 cgacttggcg cctttgatga gcgcggcgcc cgcgatcacc atgtacgcct gctcggtcat
     4981 gtagacgcgg tcggagatga tcggcatgta gccgccacct gcgatgcagt caccgaacac
     5041 gcccgcgatc tgcggcacgc cattcgcgga cagcagcgag ttcatcttga agatgtgacc
     5101 cgcgccgcgc gcaccgggga acgacttcga ttgctcgggc aagaacaggc cggagcagtc
     5161 gacgagatag atcgtcggga tgcgcaggcg cagcgccatc gtctgcgcgc gctggatctt
     5221 ttcgggcgtg cgcggccacc acgcgccgga ggcgacggtg ttgtcgttcg cgatcaccat
     5281 gcaccagcgg ccctcgatgc gcgcgaacgc ggtgacgacg ccggcggccg gcgacttctg
     5341 gtcgcccggg aagacttcgc cgtagttgac gaacgtaccg acctcgaacg tgcgggtgcc
     5401 cggatcgatc agacgcgcga tgcgttcgcg cgaggtgagc ttgcccttct tgtggacgcg
     5461 gtcgacgtac gttggtcccc agcccgcgtg gacttcggcg cggcgcgtgc gcaggcgatc
     5521 ttcgaggacc gcgagcgcgg tgcgttgatc ggcggccgtg cgctcgtcga cgagcttgcc
     5581 gagagagtcg ccgatcggaa ccagcggtac gaatgccatc tcgggcgcac ggtactacgg
     5641 cggcggccaa ttgccatctc gggcggccgc tacacctacc aatcggtagg aagctgcagg
     5701 ttcggtttct tcggcgttcg tctggttgtc ggctttgtcg gctttgtcgg ctgttcagct
     5761 ttcaacgcac agcgcgcgct gaccccgagt cgattggcac cttcgacctt cacgactcgc
     5821 tgccagatcg cacggacctt cttggcgacg actggatcct tcgcggccat gtcgcagagg
     5881 tcgtcgggct gcaccatcag gtactcccac gtgctcgcgc ggtcctccat ctggttcgtc
     5941 gtcgcgtagg tgttcacgaa gcccttcggg cgctcccagc cgacgctcgg atcgacgtac
     6001 gcgaagcctt tgggattgag cgcggcccac gccgggtctt cctcgcgatg gagcgtctcg
     6061 taatcgagca ggtgaaagac ttcgtggtgg accgcggact cgatcgacag gttcgagccg
     6121 gcgccgcgga aatactcgac gctgatgtac acgcgatgcg cgttgggatc ggcgaggccg
     6181 gccgggccgt gatcggtgcc gcgcacgtac gtgatcgcgc ggcagaacgc gacgtgctcg
     6241 agccgcgcgg tcttgcgcac gctcgacgga taacgcgcga acgcgatcgc catctggtcg
     6301 acgagctccg gctcgatctg atcgaggacg gtcgcgacat cgcagcgcgc gcagttcttc
     6361 cgcgtatcac ctggatcaca tgtggtgatc accggctggg tgtcgagctc gatgttgtcg
     6421 cgggcgagcg tcgcgcgcag gcgctggctg cttgcgacga agcctggatc gtagggcagc
     6481 gccgcggcgg gcacgcgctg agcgccaggt gtgctgcacc cgacgacaac ggcgagcccg
     6541 accagaagcc gcaacgccgg tctgcacagc acgcgacgtg ccatgcagtg ccgacgagtt
     6601 gccgccacgt tggctgtggc gccgcctgcg atcactgacg cgtcgacgcc acactgtcgc
     6661 tcgcatcacg acaggcgtgg cacgacgacg atcgcgctgc tatggaaacg gggtcacacc
     6721 cgtttgcgcg tttagtacca gtggtggcgc tgccagaacg ccttcgcgtt gtcccagccg
     6781 ccgtaccgct gattgacgta cttcgaggcg cccgcgtact gcttcgcgag gtcggtcgat
     6841 tgccagtccg cgcccatgta ttgcttgcgc gtcgccttga gcatctggaa cgcgccgaac
     6901 gcggtggagt gcgcgttctg cgcgcgcggg ttgccgctcg actcgtgctg catgatccag
     6961 cgcgccgatg cgggcatgtc gaggccgccg ttgatgtcga gaagctgttc ggagtcgagg
     7021 gtcttgaagt cgtctgacat ggctgtacgt cgtcctttcg tgtgatccga cgcagttgca
     7081 gccgcgatgc caccgacggc gcggaacccg taggatgcgt ttcgcttccc gcaggcaaca
     7141 cgacgcggtt cgacgtgcac accgtggtga tcgcgcgcgc gattcaacca tgcgatgcct
     7201 cgacgtcggc gcgatcgcgg cgtggcaact cgcgttgccg gcactggcga atcgactcgc
     7261 caagaaagcc ccagctacgg ccagccaggg atcgttcgcg tgtcgtaggc gccggtgcgg
     7321 aacgcctcgc tcttgagaat cgcgaggtgc atcgggatgg tcgtcggcac accttcgacg
     7381 acaaacgcac tcagcgcgcc gatcatcttc gcgatcgctt catcgcggtt ttcgccgcgc
     7441 acgatcagct tggcgagcag cgagtcatag aacgggggca cgacgtagcc ggtttcgacg
     7501 tgcgtgtcga cgcgaatgcc gtcgccggcc ggcggctgcc acgccgtgat cttgcccggc
     7561 gccggcgcga agttgttcgc gggatcttcg gcgttgatgc ggcactcgat gacgtgaccg
     7621 gtcagcggaa tcgcgtcctg cttccacgtc agtgggcggc ccgccgcgat ctcgagctgc
     7681 gcgatcacga ggtccttgcc ggtgcgctct tcgctcaccg tgtgctcgac ctgcagccgg
     7741 cagttcatct ccatgaagcg gagcgtgccg cccggatcga gcagcatctc catcgtgcct
     7801 gcgccgacgt agccgatgcg ggcggcggcg cgcgcggcag cctcggcgac ccggccgcgc
     7861 tggtcggcgg tgagcgcggg tgacgggctc tcctcgatca gcttctggtg gttgcgctgg
     7921 acggtgcagt cgcgctcgcc gaggtggacg gcgtgtccgt ggcggtcggc gaagatctgg
     7981 atctcgacgt ggcggccgcc ctcgatcagc ttctcgagga aca
//
LOCUS       DASUFX010000474        15044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_353887,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000474 DASUFX010000000
VERSION     DASUFX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15044
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353887"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VFV99_18880"
     CDS             complement(<1..1336)
                     /locus_tag="VFV99_18880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061443.1"
                     /translation="MEALRERFGKYHVLEKIAQGGMAEVYKVKTVGIAGFEKVQALKR
                     ILPHSARQDRFIRSFIDEARIAVELTHRNIVQVFDFGKADGELFMAMELIEGRDLRTA
                     MTQSVSRDALCPIPVAAYIIAEVAAGLDYAHRKTDGYGGALGIVHCDVSPSNVMLSTD
                     GYVKILDFGIARATFSSALERRRLRGKPRYMAPEQTFGEPPTAAADVFALGIIAWELF
                     TGLPLYRGHDVKAILDAVRRTEPPRIDRLNPDVPAEVVNAIATALSREPSQRGTASDV
                     AAACSRTAMASGARPLAAWLAVLDKATEGSVSLQSASRSGGREPTESLASVPPPPPRP
                     STQSFNTPPLRPSTRSIQPIPPADFGEGTPGPAEFHERTATVSAIIEQHPRFLTEPTT
                     TVNAPGFERATTTAPSYPQPFGERERTGSVTATKTASTTGAQKWGHEATAAGV"
     gene            1360..2073
                     /gene="rnc"
                     /locus_tag="VFV99_18885"
     CDS             1360..2073
                     /gene="rnc"
                     /locus_tag="VFV99_18885"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5061444.1"
                     /translation="MPDYSALEAVLGYAFKERELLEQALRHPSWCNEQGEPRPDDNER
                     LEFLGDAVLDLVVSHRVMTRFPKAHEGDLSVTRQQIVSEAALSEVGRQFQIGTWILLG
                     KGEEKSGGREKPKILADMFEALIGAVYLDGGYPAAWEMIDRVLGARIASVELKNFYDF
                     KTRLQETAQARLKSTPTYHVVQELGPDHDKRFVVAVTIGTDEWARAVGKSKKEAEQMA
                     AAEAHFRLEGSDIGIRPKR"
     gene            complement(2070..2924)
                     /locus_tag="VFV99_18890"
     CDS             complement(2070..2924)
                     /locus_tag="VFV99_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061445.1"
                     /translation="MNTLFWLSFFFSSPPHNTCAVSGTPLLEIRERSETSSAITTKRI
                     YSTGAWTFASDDATAQGCFSTKGLREIRSALQHAAWKVTQSPIACFAYDPNYTEYVLN
                     GRLRFTYRMCSGKTADSETMAAIDLVNRDLAAAMPPPPPPPPPPVQRPPVSACRATGV
                     PLFEIDKRSDAAVPTSTTKIYNTGAWTYLPVDKDGHAGAQQTGCFAKPTLQAIRHAIT
                     DAPWTITYSRIVCRAYSANYTVYSVGDKVEYTARLCGAQRLDDESTAAIKLIETELAA
                     VLPRQAAR"
     gene            complement(2993..3886)
                     /locus_tag="VFV99_18895"
     CDS             complement(2993..3886)
                     /locus_tag="VFV99_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5061446.1"
                     /translation="MQSPLVVGLGRVGVAISGLAPNLPSDAAPAAVLAALSTAPAPGI
                     GVALRAVVRLGCRARAVGTHGADLLGQLARTALREAGIDTELMKPAGTSACELVTVAG
                     DGNLRTRYAGDTGEPLPFDPAAAIAGAASVLIDGTAPVEQLRAAELARTQKLPVILDV
                     GELSDGTSELIAASDILIISERAASELAPRGELTDALEEIIALGPRAVVITLGEKGAI
                     GRHGEQIVRCPAFASDVLDAYGAGSVFHGAFAAALLSELPFARCIELAAAAAALSLRE
                     LGPWSAMPSRDDVLALVRTRR"
     gene            4057..4671
                     /locus_tag="VFV99_18900"
     CDS             4057..4671
                     /locus_tag="VFV99_18900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061447.1"
                     /translation="MTDAVTESMSSPVLAEPRPRVSAALSDDAQLIAACRRGEARAME
                     MLYHQFKRRVFGMAHRIVGANDAEEVAQEVFVRVFRGLANFRGDSALSTWIYRLTVNA
                     SLSHLARRGRRQEVSDENIPEQAATPEPERDPGLAARIEAAMNLLPAGYRAILVLHDV
                     EGLSHEECAAILDCRVGTCKSQLHKARARMRELLGNIAGMERSR"
     gene            4668..5402
                     /locus_tag="VFV99_18905"
     CDS             4668..5402
                     /locus_tag="VFV99_18905"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5061448.1"
                     /translation="MKCDSVREKLTAYLDGELEGERGSAVRGHLRGCDACRQVAADEA
                     ALRDGLRALPPLDPPASLWAGVQQRLAAAEVADAERPAWRRALAWLAPRAPQLGLAGA
                     ALTAAIILIVVKLNRSEETHVAQVPTAPQQPMIGADPPKKNAPVEAKDEGDMTASLAA
                     EPAQVTASYAQTARELLDIAADARAEWPDDRKHDFDAQVAAMQKKIAVATTERPRRDA
                     YRKLIRYLQRAVIEDDVALANIGGAQ"
     gene            5399..6313
                     /locus_tag="VFV99_18910"
     CDS             5399..6313
                     /locus_tag="VFV99_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061449.1"
                     /translation="MKHALVLVCVLSTSAWADVNERSRVEVQPPGKGYKQISIENALG
                     NVRIEGHDGPGIIIETHKHAPDDDALDRLRVSLVPDPDGTVRITTHADPSVESKPVPR
                     SSVSIDVVIRAPRNARFDATVGSGKLEVKNMDAGGELDSASGAITVDNVQGELYTHSV
                     SGHMSIAKAFGPVDAATISSDVELDSITGQKLVSSSNEGKIAGRRVRAREVELTTMSG
                     GITLEAEPVARGHIVVQSLRGEVDVKIRRVTQAALIVRARGTKVDFGSQPVTVLGKWH
                     EATFGSGGESALVELRSTKSLVRFAIIQ"
     gene            6360..6926
                     /locus_tag="VFV99_18915"
     CDS             6360..6926
                     /locus_tag="VFV99_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061450.1"
                     /translation="MEEPVPPRVREYAEQAVEYVRRALKVALEYDSDTLPLLDHYLRT
                     VPSDQPTTVQLVIATAGAYFGEVVRRRLGGRWEVTGEEADWRVVLPTGLNFSPVGFVA
                     SAIAQADLDDFDSEIDAPPRMRPYVQQALARMGEVSVEDYYSLCGRLDTLEHVHEVLV
                     AVAAQMMGNVDDEDVPEPVPDDPSGAVN"
     gene            6951..9788
                     /locus_tag="VFV99_18920"
     CDS             6951..9788
                     /locus_tag="VFV99_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061451.1"
                     /translation="MARGPARTPSQTAYKGTLGKTQTKTVHLTNARGDLLSTTVHTSI
                     DAVSDPELVERLYAGSLNTVRLDGQNVHDVAMPIVYHDPAAEVLVLVLGEAHRHREIE
                     ERIRLLERLREDDALVPAYVKDFAVVFGGAGLRSYLENKAQQVLASRADSKELEKKKG
                     ELVNRETELERVRQELDHAKNELKRKQGEYENSMNELRRSQLELQRDRTELERLRAEA
                     RQRVIAAAVQADKTTVAPAPTERGDDIATKPITRQEVDDIETSMVAALPPPPPDFVQE
                     ELGTQQDAPAHDIGSNGARSSSLLARFDDGEEKTGDDTKTPALGEVEVDDEPTGNTEI
                     PAGSDPLTTQTADGPLDKDKWIDAAAKAPTSVFAVVDGRARLALIAGDKIVRGMTGAL
                     DVRVLLHRLSSYPVVTMLIGPPAALRTPSPQQVVVLPLEIGNDGDRAVLNTLAKKFEL
                     TVDILQRGQTVRRVRLSAPLAENVAYIVRAADDHLRGIAADGESDASYTRARDLVLGA
                     GYDLLGVEHAEAAEFRDDKLVPLDTAQAVRRAIAIARRYSRPSREDYLVSARGYPLAR
                     WRDLRRGVLEKAVQWGIWMGPELAQIAVSEGLARSRRDLIVKLEAGFEALRRNQNAFD
                     IDADAADDNAKAIAEEAKALGVELRRKATNGGAIKSEDVAVVSGSIDGTPPKGFSRQK
                     TTDDLITLLDDKIQRVSAATELCDRGDPRAATAVVAAVKKMSRAEAVRTLGKCVKFGP
                     AAAPALIEGLSSSKAFLRHGCALALALLRTDEGTHAVIDLLLSEPTEIWREVARAIGQ
                     IGPAALMPLAAHVGRLGDGVTPSTQERVAWAMAHVGVRGGKSALDQMAAGQSVMAPIA
                     KKALELHANAARDEVRVRPGPQAAKDVTVNRAFSRRFFEALEVDRPDVAQAALHDLEA
                     SGPLEVLDESDLIVEEDEEEAELDESDLIQT"
     gene            9833..10564
                     /locus_tag="VFV99_18925"
     CDS             9833..10564
                     /locus_tag="VFV99_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5061452.1"
                     /translation="MRIGIFSDVHANIEAMNAVLDAFKSERIDKYVCIGDVVGYGASP
                     NECCDVIRKTAAFTILGNHDAAVAGRMDYSYYYDAARQALDLHARQLTPENMEWLKAL
                     PYEVREGDLTFCHGSPINLEEFEYIFSVEQATRCLEIWDDLGMVTFIGHSHLCKSFAL
                     TRDEVFEVVATKFVIRPEHRYIISVGSVGQPRDYDNRASYTIYDSNEKTFEFKRVAYD
                     IDGAAQKIFAADLERNFGNRLFLGV"
     gene            complement(10587..11873)
                     /locus_tag="VFV99_18930"
     CDS             complement(10587..11873)
                     /locus_tag="VFV99_18930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5061453.1"
                     /translation="MWFVLNLPFGATSGFVSVMLGFILKKQGMGDDVIASLVALNLLP
                     HTWKVFWAPVADSTLTRKRWYILANVTSCVTILALAFVPIEKANLGVIEWLVFLNSLA
                     ITFVGMSVEGLMAHATPPEERGRAAGMFQAGNVGGNGFGGGLGLYLAENVSTKVAFIV
                     IAIALFACQFFLRLVPEAAKIMVAAEAHAEKAAKGIAHGARILLGRIIEVFRELWQMV
                     FSRRGIVALTLCFLPIGSAAAQGIFSGQIATDWGASAELVATTSGLAAGVAATVGCLI
                     GGWMSDRMGRRMAYMAGGVILAVIAIGMAVSPQNPTMYAIYTLAYQLGSGIAYGTFTG
                     FVLDVIGKGAAATKYNALASLSNIPILYMTKILGSLSTEHGPVKMLLGDAASEIAGIT
                     VFVIVVAIVRPGKEKLPEAPEAPPEPAPLPKAKVVD"
     gene            complement(11916..13907)
                     /locus_tag="VFV99_18935"
     CDS             complement(11916..13907)
                     /locus_tag="VFV99_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX5061454.1"
                     /translation="MKVRREYRIAATTAVLAVGVPVGAACWIGARTHDLADHVGERGG
                     IHARIGAVDADLTGTIRLTDVALGDLFAAEAVEASVALDSLLAGHVSADEIRVAAPRI
                     AVEVDRDGDSDLARLVRRLGRRDPNAKPSTATHVRRIVVTSGTLTARVAGIGELSADD
                     VELVPDGHGVRLITGKLRVRAGNDRVHGEIELARSAADVSLPRVTFGRVLAVAGTGRI
                     EIGGRSVTLRDIAIGRLELGGALEARGFLDDSGVPRAVGAELVPPAPDHPGFALTVSG
                     DKVPLAPFASLAPHGLVLDGSRASGQLTVRRANATVQLAMHGSVAGVHIDHKAIAPQP
                     LAIDAALDTALAVSPDAVVVDHAALTIGAAQWSTSGWVRRGTPLSGQLDVRLATAPCN
                     DLLASLPVEVRGPLDGLTLTGTFGGRARLAIDLAAPAGEGVELDTALTNACEVTAEPP
                     AADVRKLADRPPGDKPWVELKKLDGYVPGAFISAEDGRFYDHHGFDVTQIARSLEIDL
                     RDHRLARGGSTISQQLVKNELLTHRRSLDRKIQEALLTWRLEARLAKKQILERYLNII
                     ELGPKVRGIVEAAHYWFDVSSRDLSIRQAAFLAALTSEPQTMSRRVRHAGGLDADSAA
                     RVDTVLRAMRRDGVISKDELETARDKPLRFASTALRREI"
     gene            complement(13907..14905)
                     /locus_tag="VFV99_18940"
     CDS             complement(13907..14905)
                     /locus_tag="VFV99_18940"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5061455.1"
                     /translation="MQSRKVGVLLGGLSSEREVSLRTGEAVLAALRERGHDAIPIYVD
                     HDVDVALRQEQIDVAFIALHGRMGEDGCIQGLLELLGIPYTGSDVMASALAMHKGKAK
                     ELFRLHNLPTPAYYTLSDADTADLAAIHGDFGFPCVVKPIREGSSVGVAICQSLEDLA
                     PAVDKALCFDDEVLVERFIAGKEVSVAILDDRALGAVEIAPRAGFYDYQNKYTKGATD
                     YFVPPRLSPERYRGVLAQALRAHIALGCGGATRVDMMVSDSGNEFILEVNTVPGLTPT
                     SLLPKIADAAGISFGELCELMLAGASLASRRGRGERRVVQRNFVGDDRREAAPNAH"
BASE COUNT         2586 a         5011 c         5110 g         2337 t
ORIGIN      
        1 cgacgccggc cgccgtcgct tcatgtcccc atttctgtgc gccagtcgtg ctcgcggtct
       61 tggtcgcggt cacacttccg gtgcgttcgc gctcgccgaa cggctgcgga tagctcggcg
      121 ctgtcgtcgt cgcgcgctcg aacccgggtg cgttgaccgt cgtcgtcggt tcggtcaaga
      181 accgcggatg ttgctcgatg atcgcggaca ccgtcgcggt gcgctcgtga aactcggccg
      241 gcccaggtgt gccttcgccg aagtccgcag gtggaatcgg ttggatcgat cgcgtgctcg
      301 gccgcagcgg cggcgtgttg aacgactgtg tcgacgggcg cggcggtggc ggcggcacgc
      361 tcgcgagcga ctcggtcggt tcgcggccac ccgaccgcga tgccgattgc aacgacacgc
      421 tgccctcggt cgctttgtcg agcacagcca gccatgccgc tagcgggcgc gcgccagacg
      481 ccatcgccgt ccgcgagcac gccgctgcaa catctgatgc ggtgccacgc tgacttggct
      541 cgcgcgacag cgcggtcgcg atcgcgttca cgacttccgc cggcacgtcg gggttcaacc
      601 gatcgatgcg cggcggttcg gtgcgccgca cggcgtcgag gatcgccttg acgtcgtggc
      661 cgcgatacag cggcaagccc gtgaacagct cccacgcgat gatgccgagc gcgaacacgt
      721 cggccgccgc ggtcggcggt tcgccaaacg tctgctccgg agccatgtag cgcggcttgc
      781 cgcggagccg gcgccgctcg agcgccgacg agaacgtcgc gcgcgcgatg ccgaagtcga
      841 ggatcttcac gtagccgtcg gtcgacagca tcacgttcga cggcgacacg tcgcagtgca
      901 cgatcccgag cgcgccgccg tagccgtcgg tcttgcgatg cgcgtagtcg agaccggccg
      961 cgacctccgc gatgatgtac gcagcaaccg gtatggggca cagggcatcg cgcgacacgg
     1021 actgggtcat cgcggtgcgc aagtcgcgcc cctcgatcag ctccatcgcc atgaacagct
     1081 cgccgtcggc tttaccgaag tcgaagacct ggacgatgtt gcggtgggtc agctcgacgg
     1141 cgatgcgcgc ctcgtcgatg aagctgcgga tgaaccggtc ctgccgggcg gagtggggca
     1201 ggatgcgctt gagcgcctgg accttctcga agccggcgat cccgacggtc ttgaccttgt
     1261 agacctcggc catgccgccc tgcgcgatct tctcgaggac gtggtacttc ccgaaacgct
     1321 cgcgcagcgc ttccaccgat tcacagagta tcattcctcg tgcccgatta ctccgcgctg
     1381 gaagccgtgc tcggctatgc gttcaaggaa cgcgagctac tcgagcaagc cctgcgccac
     1441 ccgtcgtggt gcaacgagca gggcgagccg cgtcccgacg ataacgagcg cctcgagttt
     1501 ctcggcgacg ccgtcctcga tctcgtcgtt agccaccgtg taatgacgcg cttcccgaaa
     1561 gcccacgaag gcgatctcag cgtcacgcgc cagcaaatcg tcagcgaagc ggcgctgagt
     1621 gaagtggggc gtcagttcca gatcggtacc tggatcctgc tcggaaaagg cgaggagaaa
     1681 agcggcggcc gcgagaagcc gaagatcctc gctgacatgt tcgaggccct gatcggcgcc
     1741 gtgtacctcg acggcggata ccccgccgct tgggaaatga tcgatcgcgt gcttggcgcg
     1801 cgcatcgcga gcgtggagct caagaacttc tacgacttca agacgcgcct ccaggagact
     1861 gcgcaggcgc ggctcaaatc gacgccgacc tatcacgtcg tgcaggagct cggtccggat
     1921 cacgacaagc ggttcgtcgt tgccgtcacg atcgggaccg acgaatgggc gcgagccgtt
     1981 ggcaagtcga agaaggaagc ggagcagatg gcggccgccg aagctcattt tcgtctcgaa
     2041 ggcagcgaca tcggcatccg cccgaagcgc tagcgcgccg cctggcgcgg cagcaccgcc
     2101 gcgagctcgg tctcgatcag cttgatcgcc gccgtgctct cgtcgtcgag ccgctgcgcg
     2161 ccacacagtc gcgcggtgta ctcgaccttg tcgccgacgg agtacacggt gtagttcgcg
     2221 gagtacgcgc ggcacacgat gcggctatac gtgatcgtcc acggcgcatc ggtgatcgcg
     2281 tgacggatcg cctgcagcgt cggcttcgcg aagcagccgg tctgctgcgc accggcatga
     2341 ccatccttgt cgaccggcag atacgtccac gcgccggtgt tgtagatctt cgttgtcgac
     2401 gtcggcaccg cggcgtccga gcgcttgtcg atctcgaaca gcggcacgcc cgtggcgcgg
     2461 cacgcactga ccggcggtcg ctgcaccgga ggaggcggcg gcggtggagg aggcggcatg
     2521 gccgcggcga ggtcgcggtt gacgagatcg atcgcggcca tcgtctcgct gtcagcggtc
     2581 ttgccgctgc acatgcgata cgtgaagcgg agcctgccgt tcagtacgta ctcggtgtag
     2641 ttcgggtcgt acgcgaagca cgcgatcggg ctttgcgtca ccttccacgc cgcgtgctgc
     2701 agcgccgagc ggatctcgcg gagccccttc gtcgagaagc agccctgcgc ggtcgcgtcg
     2761 tcgctcgcga atgtccacgc gccggtgctg tagatgcgct tcgtcgtgat cgccgagctc
     2821 gtctccgagc gctcgcggat ctcgagcagc ggcgtgccgc tgaccgcgca tgtgttgtgg
     2881 ggaggagatg agaagaagaa cgagagccag aagagcgtgt tcatgccggt agagacggca
     2941 cgtaacccaa atggatctac cgtgaatttt cggcgccgcg ctgcgtctac tactagcggc
     3001 gcgtacgcac gagcgcgagc acgtcgtcgc ggctcggcat cgccgaccac gggccgagct
     3061 cgcgtagcga cagcgcggcc gcagccgcag ccaattcgat gcagcgcgcg aacggcagct
     3121 cgctgagcag tgccgcggcg aatgcgccgt gaaacaccga gccggcgccg taggcgtcga
     3181 gcacgtcgga cgcgaacgcc gggcagcgca cgatctgctc gccgtggcgg ccgatcgcac
     3241 ctttctcgcc gagcgtgatc acgaccgcgc gcgggccgag cgcgatgatc tcttcgagcg
     3301 cgtcggtaag ctcgccgcgt ggcgcgagct cggacgcggc gcgctccgaa atgatcagga
     3361 tgtcgctggc ggcgatcagc tccgaggtgc cgtcgcttag ctcaccaacg tcgagaatca
     3421 cgggcagctt ctgcgtgcgt gccagctcgg cggcgcgcag ctgttcgacg ggcgcggtgc
     3481 cgtcgatcag caccgacgca gcaccggcaa tcgcggccgc gggatcgaac gggagcggct
     3541 cgccggtatc gccggcgtag cgggtgcgca ggttgccgtc gccggcgacc gtcaccagct
     3601 cgcacgccga cgtgccggcc ggcttcatca gctcggtgtc gatcccggct tcgcggagtg
     3661 cggtacgggc gagctgcccg agtagatccg cgccatgcgt tccgaccgca cgggcgcggc
     3721 atccgagtcg gacaaccgcc ctcagagcaa ctccgatacc cggggcgggg gccgtagata
     3781 gcgcggcgag cacggcggcc ggcgcggcat cgctcggcag gttaggagcg agccccgaga
     3841 tcgcgacccc tacacgcccg aggcccacga cgagaggcga ctgcacgatg caattctatc
     3901 agtgcgggag cgcacgtgaa agtgcttgcg acaatgggcg ttttgggcgc tagagtgcgc
     3961 cgcatcgtgt cctcggcaaa accaccgcct gctgggcaac tcagctcggc ggacgccagt
     4021 cctattgccg atgcaccgac cacggctggg gaccccgtga ccgacgctgt tacggaatcg
     4081 atgtcgagcc cggtactggc cgagccacgt ccccgcgtgt cagcagcgct ctccgacgat
     4141 gcacagctca tcgccgcctg ccggcgcggc gaggcgcgtg cgatggagat gctctatcac
     4201 cagttcaagc gccgcgtgtt cggtatggct caccgcatcg tcggcgccaa cgacgccgaa
     4261 gaagtggcgc aagaggtgtt tgtccgcgtg ttccgcggcc tcgcgaactt ccgcggcgac
     4321 tccgcgctgt cgacctggat ctatcgcctg accgtcaacg cgtcgctctc gcatctcgcg
     4381 cgtcgcggcc gccgccagga agtcagcgac gaaaacatcc ccgagcaagc ggcaacgccc
     4441 gagcccgagc gcgatccggg gctcgccgcc cgcatcgaag ccgccatgaa cctgctcccc
     4501 gcgggttatc gcgcgatctt ggtgctccac gacgtcgaag gcttgtccca cgaagaatgc
     4561 gctgccatcc tcgactgcag ggtgggcacc tgcaaatcgc agctgcacaa ggcgcgggct
     4621 cgcatgcgcg agctcttggg taacatcgcc ggaatggagc gcagccgatg aagtgcgact
     4681 ccgtccgcga gaagctgacg gcgtacctcg acggtgagct cgagggcgag cgcggcagcg
     4741 ccgtgcgcgg ccatctgcgc ggctgcgatg cgtgccgcca ggtcgccgcc gacgaagctg
     4801 cgctccgcga cggtctccgc gcgctaccac ctctcgatcc gccggcgtcg ctgtgggccg
     4861 gcgtgcagca gcggctcgca gccgccgagg tcgccgatgc cgagcgtccc gcgtggcgtc
     4921 gtgcgctcgc gtggctcgca ccgcgcgcgc cgcagcttgg cctcgccggt gcagcactca
     4981 ccgccgcgat catcctgatc gtcgtgaagc tgaaccgcag cgaagagacg catgtcgcgc
     5041 aggtgccgac cgcgccgcag caaccgatga tcggtgccga cccgccgaag aagaatgcgc
     5101 ccgtcgaggc aaaggacgaa ggcgacatga ccgcgtcgct cgccgccgag cccgcgcagg
     5161 tcacggcgag ctatgcgcag accgcgcgcg agctgctcga tatcgccgcc gacgcgcgcg
     5221 ccgagtggcc ggacgatcgc aagcacgact tcgatgctca ggtcgccgcg atgcagaaga
     5281 agatcgccgt cgcgacgacc gagcgtccgc gccgcgacgc gtatcgcaag ctgatccgct
     5341 acctccagcg cgccgtgatc gaggatgacg tcgcgctcgc gaacatcggc ggtgcgcaat
     5401 gaagcacgcg ctcgtgctcg tttgcgtgct gtcgacgtcg gcatgggccg atgtcaacga
     5461 gcggtcgcgc gtcgaggtcc agcctccggg caagggctac aagcaaatct cgatcgagaa
     5521 cgcgctcggc aacgtgcgca tcgagggcca cgatggcccg ggcatcatca tcgagacgca
     5581 caagcacgcg ccggatgacg acgcgctcga tcgtctgcgc gtgtcgctcg tgcccgatcc
     5641 ggacggcacc gtgcggatca cgacgcatgc cgacccgagt gtcgagagca agccggtgcc
     5701 acgctcgtcg gtgtcgatcg acgtcgtgat tcgggcaccg cgcaacgcgc gcttcgacgc
     5761 gaccgttggc agcggcaagc tcgaagtgaa gaacatggat gcaggcggcg agctcgatag
     5821 tgcatcgggc gcgatcaccg tcgacaacgt gcagggcgag ctctacacgc acagcgtctc
     5881 gggtcacatg tcgatcgcga aggcgttcgg cccagtcgac gcggcaacga tctcgtccga
     5941 cgtcgagctc gactcgatca ccggccagaa gctggtctcg tcctcgaatg aaggcaagat
     6001 cgccggccgc cgggtgcgcg cccgcgaggt cgagctgacg acgatgagcg gcgggatcac
     6061 gctcgaagcc gagcccgtcg cgcgcggcca catcgtggtc cagagcctgc gcggcgaggt
     6121 cgacgtcaag atccgtcgcg ttacgcaggc cgccttgatc gtccgtgccc gcggcaccaa
     6181 agtcgatttc ggatcgcaac ccgtcaccgt gcttggcaag tggcacgagg ctacgttcgg
     6241 tagcggaggc gagtctgctc tcgtcgagct gcggtcgacc aagagcctcg tccgcttcgc
     6301 gatcatccaa tagcgtaggg ctcgctccgt cccaaaagac aggctaggct gtctttgaga
     6361 tggaagagcc ggtccccccg cgggtgcgcg agtacgccga acaggcggtc gagtacgtgc
     6421 gtcgggcgct caaggtcgcg ctcgaatacg acagcgacac gctgccgctg ctcgaccact
     6481 atttgcgcac ggtgccgagc gaccagccga caaccgttca gctcgtcatc gcgacggctg
     6541 gcgcctattt cggcgaggtc gttcgccgcc ggctcggcgg ccgctgggaa gtaaccgggg
     6601 aagaggcgga ttggcgcgtc gtcctgccga ccggcctcaa cttctcgccg gttgggttcg
     6661 ttgcgtcagc cattgcacaa gccgacctcg acgacttcga tagcgaaatc gatgcacccc
     6721 cgcggatgcg gccgtacgtg cagcaggcac tcgcgcgcat gggcgaggtc tccgtcgagg
     6781 attactactc gttgtgcggc cggctcgata ccctcgagca cgtccacgag gtcctggtcg
     6841 cggtcgcggc gcagatgatg gggaacgtcg acgatgaaga tgtcccagag cccgttcccg
     6901 acgatccatc aggcgccgtg aactgagcga atcgtgttat tcctggcgac atggccaggg
     6961 gacctgctcg aacgccgtcg cagactgcct acaagggcac gctcggcaag acgcagacca
     7021 agaccgttca tctgaccaac gcgcgcggtg acctcctgtc gaccaccgtg catacgtcga
     7081 tcgacgcggt ctccgacccc gagctggtcg agcggctcta cgccggctcc ctcaatacgg
     7141 tccggctcga tggccagaac gtgcacgacg tcgcgatgcc gatcgtctat cacgaccccg
     7201 cggcagaggt gctcgtcctc gtgctcggcg aggcgcatcg ccaccgcgag atcgaagagc
     7261 gtattcgctt gctcgagcgc ctgcgcgaag acgacgcgct cgttcccgca tacgtcaaag
     7321 acttcgccgt cgtgttcggc ggtgccggcc tgcgcagcta cctcgagaac aaagcgcaac
     7381 aggtgctcgc gagccgcgcc gactccaagg aactggagaa gaagaagggc gagctggtca
     7441 atcgcgagac cgagctcgag cgcgttcgcc aggagctcga tcacgcgaag aacgagctca
     7501 agcgcaagca gggcgagtac gagaactcga tgaacgagct ccgccgctcg cagctcgagc
     7561 tgcagcgcga tcgcaccgag ctcgagcggc tacgcgccga ggcgcgccag cgcgtgatcg
     7621 cggcagcggt acaggcggac aagacaaccg tggcgccggc gccgaccgag cgcggtgatg
     7681 acatcgcgac gaagccgatc acgcggcaag aggtcgacga catcgaaacc agcatggtcg
     7741 ccgcgctgcc gccacctccg ccggacttcg tccaggagga gcttggaacg cagcaggatg
     7801 cgccagccca cgacatcggc agcaatggcg cgcgctcgtc gagcttgctc gccaggttcg
     7861 acgatggcga agaaaagacc ggcgacgaca ccaagacgcc ggccctcggc gaggtcgagg
     7921 tcgacgacga accgaccggc aacaccgaga ttccggcggg ctccgatccg ctgaccacgc
     7981 agacggccga cgggccgctc gacaaggaca agtggatcga cgccgcggcg aaggccccga
     8041 cgtcggtgtt cgccgtcgtc gatggccgtg cgcgcctcgc gctgatcgcg ggagacaaga
     8101 tcgtgcgcgg catgaccggc gctctcgacg tccgcgtgct cttgcatcga ctgtcgtcgt
     8161 atccggtcgt cacgatgctg atcggaccac cggcggcgct gcgcacgccc agcccgcaac
     8221 aggtcgtcgt gctcccgctc gaaatcggca atgacggcga tcgcgcggtg ctgaacacgc
     8281 tcgccaagaa gttcgagcta acggtcgaca tcttgcagcg cggccagacc gtgcgccggg
     8341 tgcggctcag cgcgccgctc gccgagaacg tcgcgtacat cgtgcgtgcg gccgacgatc
     8401 acctgcgggg aatcgccgcc gacggcgagt ccgacgcttc gtacacgcgc gcccgcgacc
     8461 tcgtgctcgg cgcgggctac gatctgctcg gcgtcgagca cgccgaagcc gcggagttcc
     8521 gcgacgacaa gctcgtaccg ctcgacaccg cgcaggccgt gcgtcgcgcg atcgcgatcg
     8581 cccggcgcta ctcgcggccg tcgcgcgagg actacttggt atccgcgcgt ggctatccgc
     8641 tcgcgcgctg gcgggacctg cgccgtggtg tgctcgagaa ggcggtgcag tggggcatct
     8701 ggatgggccc cgagctcgcc cagatcgccg tctccgaagg actcgcacgc tcccggcgcg
     8761 acctgatcgt gaagctcgag gctggcttcg aggcgctgcg ccgcaatcag aacgcgttcg
     8821 atatcgacgc cgatgccgcc gacgacaacg caaaggcgat cgccgaggag gcgaaggcgc
     8881 taggcgtcga gctgcgccgc aaggcgacga acggcggtgc gatcaagagc gaggacgtcg
     8941 cggtcgtctc gggctcgatc gacggcacgc cgccgaaggg cttctcgagg caaaagacca
     9001 ccgacgacct catcacgctg ctcgacgaca agatccagcg cgtgtcggct gccaccgagc
     9061 tgtgtgaccg cggcgatccg cgggccgcca ccgccgtcgt cgccgccgtc aagaagatga
     9121 gccgcgccga ggccgttcgc acgctcggca aatgcgtgaa gttcggaccc gcggccgcgc
     9181 cagcgctgat cgagggtctg tcgagcagca aggcgttctt gcgccacggc tgcgcgctcg
     9241 cactggcact gctccgtacg gacgagggca cccacgcggt gatcgacctg ctcttaagcg
     9301 agcccaccga aatttggcgc gaggtcgccc gcgcgatcgg ccagatcggc ccagccgcgc
     9361 tgatgccgct cgctgcgcat gtcggccgcc tgggggacgg ggtaacaccg tcgacgcaag
     9421 agcgcgtcgc ctgggcgatg gctcacgtcg gcgtccgcgg cggcaaatcc gccctcgacc
     9481 agatggcggc ggggcagagc gtcatggcgc caattgccaa gaaagcgctc gagctgcacg
     9541 ccaacgccgc tcgcgacgag gttcgggtgc gcccgggccc gcaggcggcc aaggatgtca
     9601 ccgtcaatcg tgcgttctcg cgccgcttct tcgaagcgct cgaggtcgat cgcccagatg
     9661 tcgcacaagc ggcgcttcac gacctcgagg ccagcggtcc gctcgaagtt ctcgacgaat
     9721 ctgacctgat cgtcgaggag gacgaggaag aggccgagct cgacgaatcg gatctgatcc
     9781 agacctagct aaccggaatc gcccgacttc aggtatcatt agcgagccgt acatgaggat
     9841 cgggatcttc tccgacgtgc acgcgaacat cgaagcgatg aacgcggtgc tagatgcgtt
     9901 caagtccgag cgcatcgata agtatgtatg catcggagat gtcgtcggat acggcgcgtc
     9961 cccgaacgag tgttgcgacg tgattcgcaa gacggccgcg ttcacgattc tcggcaatca
    10021 cgatgccgcg gtcgccggcc ggatggacta ctcgtactac tacgacgcgg cccgccaggc
    10081 cctcgatctt cacgcgcgtc agctcacgcc cgagaacatg gagtggctga aggcgctgcc
    10141 gtacgaggtc cgcgaaggcg acctgacgtt ctgccacggc tcgccgatca acctcgaaga
    10201 gttcgagtac atcttctcgg tcgagcaagc gacccgctgc ctcgagatct gggacgacct
    10261 cggcatggtc acgttcatcg gccactcgca cctgtgcaag tcgtttgcgc tcacgcgcga
    10321 cgaggtgttc gaggtcgtgg cgacgaagtt cgtgatccgg cccgagcacc gctacatcat
    10381 ctcggtgggc tcggtcggcc agccgcgcga ttacgacaac cgcgcgagct acacgatcta
    10441 cgactcgaac gagaagacgt tcgagttcaa gcgcgtcgcg tacgacatcg acggtgcggc
    10501 gcagaagatc ttcgccgccg acctcgagcg caacttcggc aaccggctgt tcctcggcgt
    10561 ctgacgacgc tgccggggct cgggggctag tcgactacct tcgccttggg taatggcgcg
    10621 ggctcgggcg gtgcctcggg tgcctcgggc agcttctctt tgccggggcg cacgatcgcg
    10681 acgacgatca cgaacacggt gatgcctgcg atctcggagg cggcgtcgcc caacagcatc
    10741 ttgaccggac cgtgctcggt cgagagtgag ccaagaatct tggtcatgta gaggatcggg
    10801 atgttcgaca gcgatgcgag cgcgttgtac ttcgtggccg cggcgccctt gccgatcacg
    10861 tcgagcacga aaccggtgaa cgtgccgtac gcgatgccgc tgccgagctg ataggcgagc
    10921 gtgtagatcg catacatcgt cggattctgt ggcgacaccg ccatcccgat cgcgatcacc
    10981 gccaggatca cgccgcccgc catgtaggcc atgcggcggc ccatccgatc cgacatccag
    11041 ccgccgatca aacagccgac cgtcgctgca acgccggcgg cgagaccact ggttgtcgca
    11101 acgagctcgg cgctcgcacc ccagtccgtc gcgatttgcc ccgagaagat gccctgcgcg
    11161 gcggcagagc cgatcggcag gaagcacagc gtgagcgcga cgatgccgcg gcgcgagaac
    11221 accatctgcc acagctcgcg gaacacctcg atgatgcggc cgagcaggat gcgcgctccg
    11281 tgcgcgatac ccttcgccgc cttctctgca tgcgcttctg cggcgaccat gatctttgcc
    11341 gcctcgggca cgaggcgcag gaagaactga cacgcgaaga gcgcgatcgc gatgacgatg
    11401 aacgcgacct tcgtcgagac gttctcggcg aggtagagac cgagaccgcc gccgaagccg
    11461 ttaccgccga cgttaccggc ctggaacatg ccagcggcgc gtccgcgctc ttcgggcggc
    11521 gtggcatgcg ccatgaggcc ctcgaccgac atgccgacga acgtgatcgc gaggctgttc
    11581 aggaagacaa gccactcgat cacgccgagg ttggccttct cgatcgggac gaacgcgagc
    11641 gcgaggatcg tcacacacga ggtgacgttc gcgaggatgt accagcgctt tcgcgtcagc
    11701 gtgctgtcgg cgacgggtgc ccagaacacc ttccacgtgt gcggcaatag attcagcgcg
    11761 acgaggctcg cgatcacgtc gtcgcccatc ccctgtttct tgaggatgaa gccgagcatc
    11821 acggagacga aaccactcgt ggcgccgaac ggcaggttca ggacgaacca cacccacggt
    11881 ctgactcggc gttgctctgt gctcactaac gagcgttata tctcgcggcg gagcgcggtc
    11941 gaggcaaatc gcagcggttt gtcgcgcgca gtctctagct cgtctttgct gatcacgccg
    12001 tccctgcgca tcgcgcgcag caccgtgtcg acgcgggcgg cactatcggc atcgaggcca
    12061 ccggcgtggc ggacgcgacg gctcatcgtc tggggctcgg acgtcagcgc ggcgaggaac
    12121 gcggcttgcc ggatcgacag gtcacgcgac gacacgtcga accagtagtg cgcggcttcc
    12181 acgatgccgc ggaccttcgg gccgagctcg atgatgttga ggtagcgctc gaggatctgt
    12241 ttctttgcga gccgcgcttc gaggcgccag gtcagcagcg cttcctggat cttccgatcg
    12301 aggctccgcc gatgtgtgag cagctcgttc ttcaccagct gctggctgat cgtcgagccg
    12361 ccgcgtgcga gacggtggtc gcggaggtcg atctcgaggc tgcgtgcgat ctgcgtgacg
    12421 tcgaagccgt gatggtcgta gaagcggccg tcttcggccg agatgaacgc accgggcacg
    12481 tacccatcga gcttcttcag ctcgacccat ggtttatcgc caggcgggcg atcggcgagc
    12541 ttgcgcacgt ccgcggccgg aggctcggca gtgacttcgc atgcgttcgt cagtgccgtg
    12601 tcgagctcga cgccttcgcc cgcgggtgcc gcgagatcga tcgcgaggcg ggcgcgcccg
    12661 ccaaacgtgc cggtgagcgt cagcccgtcg agcggtccgc gcacttcgac cggcagcgac
    12721 gcgagcaggt cgttgcaggg cgccgttgcg agccggacgt cgagctgacc cgagagcggc
    12781 gtgccgcggc gcacccagcc tgacgtcgac cactgcgcgg cgccgatcgt tagcgcggcg
    12841 tgatcgacga cgacggcgtc gggcgagacg gcgagggcgg tatcgagtgc ggcgtcgatc
    12901 gcgagcggct gcggcgcgat tgccttgtga tcgatgtgca cgccggcgac cgagccgtgc
    12961 atcgcgagct gcaccgttgc gttggcgcgg cgtacggtga gctgaccgga ggcgcggctg
    13021 ccgtcgagca cgagcccgtg cggcgcgagc gacgcgaacg gcgcgagtgg aaccttgtca
    13081 ccgctgacgg tgagtgcgaa cccgggatgg tcgggcgccg gtggcacgag ctcggcaccg
    13141 acggcgcgcg gcacgccgct atcgtcgaga aagccgcgcg cttcgagtgc accgccgagc
    13201 tcgaggcgac cgatggcgat gtcgcgcagc gtgaccgagc ggccaccgat ctcgatgcga
    13261 ccggtccccg cgactgcgag cacgcggccg aacgtgacgc gcggaagcga gacatccgcc
    13321 gcgctgcgtg cgagctcgat ctcgccgtgc acgcgatcgt tgccggcgcg cacgcgcaac
    13381 ttgccggtga tcagacgaac gccgtggccg tcgggaacga gctcgacatc gtcagccgac
    13441 agctcgccga tgccggcgac gcgcgcggtg agcgtgcccg acgtgacgac aatgcggcgc
    13501 acgtgcgtgg ccgtgctggg cttcgcgttc ggatcgcgac gaccgagccg gcggacgagg
    13561 cgcgcgagat ccgaatcacc atcgcgatcg acctcgaccg cgatgcgtgg cgccgcgacg
    13621 cgaatctcgt cggcggagac atgacccgcg agcagcgagt cgagtgcgac cgaggcttcg
    13681 acggcttccg cggcaaacaa gtcgccgagc gcgacgtcgg taagccggat cgttccggtg
    13741 aggtccgcgt cgacggcgcc gatgcgcgcg tgaatgccgc cacgctcgcc gacgtgatcc
    13801 gcgaggtcgt gcgtacgcgc gccgatccaa catgccgcgc caaccggaac gccgaccgcg
    13861 agcacggcgg tcgtcgcagc gatccgatat tcgcggcgga ccttcatcag tgagcgttcg
    13921 gtgcggcctc tcggcgatcg tcgccgacga agttgcgctg gaccacgcga cgttcaccgc
    13981 gaccgcgccg cgacgcgagc gacgcgccgg cgagcatcag ctcgcacagc tcgccgaacg
    14041 agatgccggc ggcatccgcg atctttggca acaggctcgt cggggtgaga ccgggaaccg
    14101 tgttgacctc gaggatgaac tcgttgcccg agtcgctgac catcatgtcg acgcgcgtcg
    14161 cgccgccgca accgagcgcg atgtgtgcgc ggagtgcctg cgcgagaacg ccgcgataac
    14221 gctcgggcga caggcgtggc gggacgaagt agtcggtcgc gcccttcgtg tacttgttct
    14281 ggtagtcgta gaagcccgcg cggggcgcga tttcgacggc gccgagtgca cggtcgtcga
    14341 gaatcgcgac cgagacttcc ttgccggcga tgaaccgctc gaccaggacc tcgtcgtcga
    14401 agcacagcgc cttgtcgacg gccggcgcca gatcctcgag tgactgacag atcgcgacgc
    14461 cgaccgagct gccttcgcgg atgggcttca ccacgcacgg gaaaccgaag tcaccatgga
    14521 ttgccgcaag atctgcggtg tccgcgtcac tgagcgtgta atacgcgggg gtaggtaggt
    14581 tgtggaggcg gaacagctcc ttcgccttgc ccttgtgcat cgccagcgcc gacgccatca
    14641 cgtccgagcc ggtgtacggg attccgagta gctcgagcag gccttggatg cagccgtctt
    14701 cgcccatccg cccgtgaagt gcgatgaaag cgacatcgat ctgctcctgg cggagggcga
    14761 catcgacgtc gtggtcgaca tagatcggga tggcgtcgtg gccgcgctcg cgcagggctg
    14821 cgaggaccgc ctcgccggtc cgcagcgaga cctcacgctc actggagagg ccgccgagca
    14881 aaacgccaac tttccgggac tgcatatccg agctccttta cagacgagaa cgtcgcagga
    14941 tcccgccgta atccgaccgt aaaggccgat gatccgaggg gcaatttttc ggctcctttc
    15001 gtggtaagga agggccgcga tggcgaagaa gaaaacagcc gacg
//
LOCUS       DASUFX010000475         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1744184, whole genome shotgun sequence.
ACCESSION   DASUFX010000475 DASUFX010000000
VERSION     DASUFX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744184"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1494
                     /locus_tag="VFV99_18945"
     CDS             184..1494
                     /locus_tag="VFV99_18945"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5061456.1"
                     /translation="MRPIRKIVVLGANGAMGSGSGAVFASAGIPTVFLARSLEKAEAG
                     RARAEQLAKGKVPPKSISVGTYDADLAHSVAEADLIFEAVAEDLETKRKVFALIDRAR
                     TPGSIVATVSSGLSIADMCASCTEDFRRHFLGVHLFNPPTLITGTEIIPHAKTDRAVV
                     EMIRQVLEATFGRVVIECKDTPAFAGNRIGFKLLNEIAQLAEEHGVAYMDQLVGTHTG
                     RALAPLATIDLVGWDVHKAIVDNLYANTRDEAHDTFVLPAYMARGIERGHLGRKTRDK
                     GGFFRVDGKGPDAQHFVLDPKTGDYKPLAELKPPVPAFIERMKAAIKSGQHPEAFDIL
                     CRADGKDATLLRRVMLGYISYSLGRVGEVVAEARDVDRIMGFGFNWAPPSVLVDAIGA
                     ARTIELLEKEQLPVPAVIAAAAASDGKPMFAEPSVDTGRFFVAA"
     gene            complement(1491..2210)
                     /locus_tag="VFV99_18950"
     CDS             complement(1491..2210)
                     /locus_tag="VFV99_18950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5061457.1"
                     /translation="MAVAAEPRHFTLDYVERATLRDGTPVMLRLICPEDKELLRREFE
                     RWSPESRYSRFHAAKTKLTDEELAYLTDVDHEGHFALCAIREAGDGHGEPVGLGIARF
                     IRLPEELGAPVTAEAAISVADHAQGQGLGRLLFLRLVAAAAERGIERFRCEVLGSNAS
                     MAGLIEAIAPDHHTEVASGVMSIDFAVPAMKPDVAPSEPPEGGMYRLFRAVAEGAVTL
                     ARRIADVVDNVVDSDEPPKPD"
     gene            complement(2201..2962)
                     /locus_tag="VFV99_18955"
     CDS             complement(2201..2962)
                     /locus_tag="VFV99_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX5061458.1"
                     /translation="MNLDAYFDRIGWHGSRAATTEVLAPVLDHHMRAIPFENLDVLLG
                     DKPRLDIGSLERKLVERRRGGYCYEHATLFAAMLTELGFAVKTHSARVVMVTARSSAP
                     RTHMFLSVGDLVLDPGFGGAAPRVPVPLDGTQAGAHRLSREGSEYVLEQDGQRLWMSS
                     LEHDIPIDFEMANHFTATHPSSPFTQGLMMRAYTDDGGQVRVRGRDVTLMRGAETQTY
                     QLENRKQLRELVAAHFGFDLPAIESMHVPMVPEWR"
     gene            3142..>3576
                     /locus_tag="VFV99_18960"
     CDS             3142..>3576
                     /locus_tag="VFV99_18960"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX5061459.1"
                     /translation="MQIPVDITFKELGKSDALETRIRESVQKLERVFDRIVRCEVLVE
                     TPHRHHRNGRQYHVRIRLTVPGGEIVVSRDPGPDEAHEDAYVALRDAFLAARRQLEDY
                     VRKTLKRGDQRAERNGSAHARVTFIDVEKSWGWLEPDDGRRVY"
BASE COUNT          623 a         1162 c         1147 g          644 t
ORIGIN      
        1 tcgatgacga tggcgccgaa acgtcgtcac cgatcggtcg caaccccaac ttcggtcgca
       61 ccaccgcgcg ctatgcgccc ggttcggcgc aggtgggctt ccgtgtcacg ttctagccac
      121 gaagcttctt taccttttaa aggtctttcg gccggtatgc gttttgctgt ctcatcttcg
      181 accatgagac ccatccgtaa gatcgttgtc cttggtgcta acggagcgat ggggtcggga
      241 tcaggcgcgg tgttcgcttc cgcaggcatc ccgacggtct ttctcgctcg ttcgctcgag
      301 aaagcagaag ccggtcgcgc gcgcgcagag cagctcgcga aaggcaaagt accgccgaag
      361 tcgatcagcg tcggcacgta cgacgcggat ctcgcgcatt cggtagccga ggcggacctc
      421 attttcgagg ctgtcgcgga agatctggag acgaagcgta aggtgttcgc gctcatcgat
      481 cgcgcacgta cgccgggctc gatcgtagcg acggtatcgt cgggtctgtc gatcgcggac
      541 atgtgcgcca gctgcacgga agacttccgc cgtcacttcc tcggcgtgca cctgttcaat
      601 ccaccgacgc tgattaccgg tacggagatc attccgcacg cgaagaccga tcgcgcggtc
      661 gtcgagatga tccgtcaggt gctcgaagcg acgtttggtc gcgtcgtgat cgagtgcaag
      721 gacacgccgg cgtttgcggg taatcgcatc ggcttcaagc tgctcaacga gatcgcgcag
      781 ctcgccgaag agcatggcgt cgcgtacatg gatcagctgg tcggcacgca taccggccgc
      841 gcgctcgcgc ccctcgcgac gatcgatctt gtcggctggg acgttcacaa ggcgatcgtc
      901 gacaacctgt acgcgaacac gcgcgacgaa gcgcacgaca cgttcgtgtt gcccgcttac
      961 atggcacgcg gtatcgagcg cggtcacctc ggtcgcaaga cgagggacaa gggcggcttc
     1021 ttccgggtcg acggcaaggg ccccgatgcg cagcacttcg tactcgatcc gaagaccggc
     1081 gattacaagc cgcttgccga gctcaagccg ccggtgccgg cgttcatcga gcgcatgaag
     1141 gctgcgatca agagcggcca gcatccggag gcgttcgaca ttctgtgtcg cgccgacggt
     1201 aaggacgcca cgctgctgcg ccgcgtgatg ctcggctaca tcagctactc gctcggtcgc
     1261 gtcggcgagg tcgtcgcgga agcgcgcgat gtcgatcgca tcatgggctt tggcttcaac
     1321 tgggcgccgc cgagcgtgct cgtcgatgcg atcggcgcgg cgcgtacgat cgagctgctc
     1381 gagaaggagc agctcccggt acccgccgtc atcgccgccg cagccgccag cgatggtaag
     1441 ccgatgtttg ccgagccgag cgtcgacacc ggccgcttct tcgtcgcggc ctaatcaggc
     1501 ttcggcggct cgtcgctatc gacgacgtta tcgacgacgt ccgcgatgcg gcgcgcgaga
     1561 gtgaccgcac cctcggcgac tgcgcggaac agccggtaca tgccgccttc gggtggctcg
     1621 ctcggcgcga cgtcgggctt catcgccggt accgcgaagt cgatcgacat cacgccgctc
     1681 gcgacctcgg tgtgatggtc gggcgcgatc gcctcgatca ggcccgccat cgacgcgttg
     1741 ctaccgagta cctcgcagcg aaagcgttcg ataccgcgct cagcggcggc cgcgacgagg
     1801 cgtaggaaca gcaagcgacc gagtccctgc ccttgcgcgt gatcggctac cgagatggcc
     1861 gcttccgcgg tcaccggtgc gccgagctcc tccggcaagc gaatgaagcg cgcgataccg
     1921 aggccgaccg gctcgccgtg gccgtcaccc gcttcgcgga tcgcacagag tgcgaagtgg
     1981 ccttcatgat cgacgtcggt gagatatgcg agctcctcgt cggtcagctt cgtcttcgct
     2041 gcgtgaaacc gcgaatatcg agattccggc gaccagcgct cgaactcgcg gcgcaacagc
     2101 tctttgtcct ccggacaaat caggcgcagc atcaccggcg tgccgtcgcg cagcgtcgcg
     2161 cgttcgacgt agtccagcgt gaaatgccgc ggctctgcag ctaccgccat tccggaacca
     2221 tcggcacgtg catcgattcg atcgccggca ggtcgaagcc gaagtgtgcg gcgacgagct
     2281 cgcgcagctg cttgcgattc tcgagctggt acgtctgcgt ttcggcgccg cgcatcagcg
     2341 tgacgtcgcg accgcgtacg cgaacctgac cgccgtcatc ggtgtacgcg cgcatcatca
     2401 agccctgcgt gaacggtgag gacggatgcg tcgcggtgaa gtgattggcc atctcgaagt
     2461 cgatcgggat gtcgtgctcg agcgacgaca tccacagccg ctgaccatct tgctcgagta
     2521 cgtactcgct gccttcgcgc gacaagcgat gtgctcccgc ctgcgtgccg tcgaggggca
     2581 ccggtacgcg gggcgctgcg ccgccgaatc cgggatcgag caccaagtcg cctaccgaca
     2641 agaacatgtg cgtgcgaggg gccgagctac gcgccgtgac catcaccacg cgcgcactgt
     2701 gcgtcttcac cgcgaaacca agctcggtca gcatcgcggc aaatagcgtc gcgtgctcgt
     2761 agcagtagcc accgcgtcgc cgctcgacga gcttgcgctc caggctgccg atgtcgagtc
     2821 gcggtttgtc gccgagcaac acgtcgaggt tctcgaacgg gatcgcccgc atgtgatggt
     2881 cgaggaccgg cgcgagcacc tcggtggttg ccgcgcgcga gccatgccag ccaatccgat
     2941 cgaaataggc gtcgaggttc acgaccgcga gactaccagt acccgctgca tttgcgcccg
     3001 atgcgaattc ttcccgcgcg agtttccccg cggattctgc gaacgtgcgc caccgccacg
     3061 cgatccttgc gcgacttgcg ctcgaaacgt gcgtttcgcc gggcgcttag cgtggcctac
     3121 gcgatgcaca ccgtttagat catgcagatt cccgtcgaca tcacgttcaa agagctcggg
     3181 aaaagtgacg cactggagac tcggatccga gagtcggtcc aaaagctgga gcgcgttttt
     3241 gatcgcatcg tgcgctgcga ggtcctggtc gagacgccgc atcgccacca tcgcaacggt
     3301 cgccaatatc acgttcgcat tcgactcacc gtgccgggcg gtgagatcgt cgtcagccgc
     3361 gacccgggtc cggacgaggc acacgaggat gcgtacgtcg cgctgcgcga tgcgttcctc
     3421 gcggctcgtc gccagctcga agactacgtt cgcaagacgc tcaagcgcgg tgaccagcgt
     3481 gccgagcgga acgggtcggc gcatgcacgc gtgacgttca tcgatgtcga aaaatcgtgg
     3541 ggctggctcg agcccgacga cggccgtcgc gtttac
//
LOCUS       DASUFX010000476         8934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_354569,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000476 DASUFX010000000
VERSION     DASUFX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354569"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFV99_18965"
     CDS             complement(<1..481)
                     /locus_tag="VFV99_18965"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5061460.1"
                     /translation="MSATTKEPSLWLGTRRDDGGGAVALPARALLRHAIALGSSGSGK
                     TVFCKVVVEEAIRLGVPVIAIDPQGDLASLALGPVADDELARHGGDVTIAAELRERAD
                     VVVFTPASHKGVALCADPVDADVARLAAEDRPQSVTRSASRIVALLGYDLDSDEGAGV
                     "
     gene            510..1247
                     /locus_tag="VFV99_18970"
     CDS             510..1247
                     /locus_tag="VFV99_18970"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061461.1"
                     /translation="MTRLLAIAVLLIGCATTAHVPKAVVVVSANMEWKAVTAAYPDAR
                     MESTPWGDTFETTIGRDRVVMFHGGWGKVAAAGSAQYAIDRWHPRYVVNLGTAGGFAG
                     AIERHSIVLVDRTVIYDIVEAMGDSAEAIASYATAIDLSWLPTPYPSAVTKTLLVSAD
                     RDIVPAQLSELATRYKAVAGDWESGAIAYTCARNKQRVLILRGISDLVHTEGGEAYGN
                     MPAFEEGARIVMQRLLRELPAWLDRMP"
     gene            complement(1244..1669)
                     /locus_tag="VFV99_18975"
     CDS             complement(1244..1669)
                     /locus_tag="VFV99_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061462.1"
                     /translation="MKLMFPLLFVVACAGSQKEVTMQGSDLDIARVAGEWQGEYKGDD
                     SGRTGQVSFSLEVGSHIAEGTVMMGPKPLKIEFVKVSRNEVKGTIAPYTDPQCSCEVE
                     TSFLGTLNDDSIYGTFESRLSSTGQLQAGTWSVQRKPRT"
     gene            1890..2666
                     /locus_tag="VFV99_18980"
     CDS             1890..2666
                     /locus_tag="VFV99_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061463.1"
                     /translation="MGFSFASVVLSYFLIAGGTFFTMLLALRLGLHSEYLGYIILAAG
                     GFLGGMVAARASKGSTIIEPAIGAVLLLASFIGVGLAVSGKDVSVVVLPGTMKAIGLT
                     ALASAGGGIGGAFMTEKMFGDENASGASWVLFVALAAVGAGIIGTLFGGVLAKGDAGN
                     TIFGLIAVCTLFIGIATGASATSRPLGASFLGGAIGVGGFFFLAIYLLLSMFSDKSHG
                     AGDIPSVVYAGIAVVAVGAGIVTLIGALFGWAIVGSKQNA"
     gene            complement(2846..5113)
                     /locus_tag="VFV99_18985"
     CDS             complement(2846..5113)
                     /locus_tag="VFV99_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061464.1"
                     /translation="MSARQTRGLRIRTQCKTLDELVRVFHRFVDETSIFVPTPSPRQE
                     GIEAAFSIDLASGDSALVGQGVVLGAWTTRDNRFGQAGMQIGVRKVTPASVKVYEQML
                     IARALAKDSKESAPESWEDGTDIATPAFDDRQAVPQRAIDLVSAPIEGAPIVSSKHTL
                     LGLPIIPHAPASPASEPNTAAPVVAVPRVATKPMVSGGVKPPTVVVTIPATVAPKPAT
                     AVAAKPVAAVAARPATAAATRATAAMASVSPAVEPMITVKPKLSPNAFVGVPARIAPK
                     NGAAPAAQPSPIKARPEIARASEPAMPRPVKKPSTLTSGWDIVDPAPQPEVKPGKLTM
                     GWDAQPEPTPQQEKPRAEVKPLAPLAPVTPLAPVAPVAPLAPVVARGSRPAMKDPEPV
                     ADAVPEPITLVPPESLAAEPAIPEPVPAAPEPIEAESAASEPEAETKPGRPRLGTVLG
                     SGPVPRLPAGMDGAADVFEPPYEIPDEESSAPQAVDPELTERDVVNHFALAAHGLAAP
                     VEVAPIAVAVPVDEPSINEPLPLVEPPPIVPAPVIDAVPVIDAAPVVEAAPDVDVDVD
                     VPVLEPAPVVEAPVVPVEAVPEPMRFLETQPVVRRRRRRRVGLAIALVAIVCAGGGFA
                     AGLIAQQQRTQAAVEAPSVDVASFEAPAEVPTSDVALEPVVPLLQFDAGSAAAPVAVV
                     ATPVAAAPVAVPPVAVAPPSEAPVAEARVEPPSAKPLPTATRVKRPIRTPTRPYVAPR
                     PKAKAKRACDDLSCL"
     gene            5527..7221
                     /locus_tag="VFV99_18990"
     CDS             5527..7221
                     /locus_tag="VFV99_18990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061465.1"
                     /translation="MRPLSEDADNEFCEDTGGSEIRDAMRATSAFRLLVVIDGAAVTH
                     DLPAHGRVTLGRARGSHVPIAHVSVSRDHAFLDLAPLAIVDRNSRNGTYVRGAAIDAG
                     VPVMIAVDEPFRIGDVMVMVQSRIALGSVSGISDGAPSPVNLVAECARSVRTGAAFAF
                     ARILVDPAVTERACEALMGTVRVSDQLSQAPGELRVLFTDTPATSLGTVVDRLLSALD
                     RAGISARVGAAGFPIDGTTPIQLAARTWQQLLRGQGAPDPIAPVRDTLARLAVGDLSL
                     LLCGETGVGKEVYAEIIHRASLRSRGPLIKVNCAALTETLLESELFGHERGAFTGAAS
                     AHAGLIEAADGGTLFLDEIGELPLMLQAKLLRVLEDREVRRVGGTSGRQIDVRFVAAT
                     NRSLEAEVAAGRFRRDLLFRIAGATITIPPLRQRRDDIEAIARAVIEQARTGGGAVLS
                     YDAIVALRNWHWPGNVRELRNTIERALLVARNGTIVAHDLGLETEIEVTGVDLLRRRE
                     GQAPLGASVEAHEREQILAALEHCGGNKTQAARMLGIARNTLLARLDAWGLRDGKK"
     gene            7380..8255
                     /locus_tag="VFV99_18995"
     CDS             7380..8255
                     /locus_tag="VFV99_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061466.1"
                     /translation="MHKILAALPLVLFVACVDEEPITQAPAIEDPGADALNVTITDTT
                     ATGSFQASDSVVTFGVETVSPAVYDMWVELHGLHLTLLVDAEVGVLEFDGYAADTGAL
                     TQMTEADRATLLELYKALDVYGNDYEHVSLLRRKVGNWAETSDTMNLTFQHLFDRDQS
                     WTSLCGSCGRWVSASHDCNHGGWWSDATTVNALIKYEGAWCGGDGSAYGTTTSVSCTG
                     EPAGFTTYEYTRGNCMGRCGPGCNSSDTQFTQDCVNHDSCVRGGHDLASAYCDDQFAS
                     CTDDDVSAPHCGTGC"
     gene            8502..8732
                     /locus_tag="VFV99_19000"
     CDS             8502..8732
                     /locus_tag="VFV99_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061467.1"
                     /translation="MDPKPATREQPLTEEERDPRMKRSIEMLDQAVARTAAKLAAAEA
                     AGDKVAANTARVRIKRLQEVRKQRASELEATR"
     gene            8729..>8934
                     /locus_tag="VFV99_19005"
     CDS             8729..>8934
                     /locus_tag="VFV99_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061468.1"
                     /translation="MSATTKLVAVVLAIAAGCTEAPAETPGSDAVRNYFAAVTAKDCS
                     ALGTLSGGKVAKNLERLGCDKLIEG"
BASE COUNT         1483 a         2913 c         2978 g         1560 t
ORIGIN      
        1 ggacgccggc accttcatcg ctgtcgagat cgtagccgag cagcgcgacg atgcgcgacg
       61 ccgagcgtgt caccgactgg ggcctgtcct ccgcggcgag gcgtgcgaca tcggcgtcga
      121 ccggatcggc gcacagcgcg acacctttgt gcgacgccgg tgtgaacacg acgacgtctg
      181 cgcgctcgcg cagctccgcg gcgatggtga cgtcgccgcc gtgccgcgcc agctcgtcat
      241 cagcgaccgg gccaagcgcg aggctcgcga ggtcgccttg cggatcgatc gcgatcaccg
      301 gcacgccgag gcggatcgct tcttcgacga ccactttgca gaacacggtc tttccagacc
      361 cgctcgagcc aagggcgatc gcatggcgta gcagcgcgcg tgccggcagc gcgacggcgc
      421 cacctccgtc gtctcggcgc gttcccagcc acaaagacgg ctctttggtc gtggcggaca
      481 tcggcagctg ggactgtaca atcacccgcg tgacgaggct gctcgcgatc gcagttctgt
      541 tgatcggctg cgcaactacg gcccacgtgc ccaaagccgt cgtcgtggtg tcggcgaaca
      601 tggaatggaa agcggtgacg gccgcctatc ccgacgcacg aatggaatcc actccgtggg
      661 gcgacacgtt cgagaccacg atcggccgcg accgcgtcgt catgtttcac ggtggctggg
      721 gcaaggtcgc cgcagccggc tcggcgcagt acgcgatcga ccgctggcac ccgcgctacg
      781 tcgtcaatct cggaacggcc ggtgggttcg caggtgcgat cgagcgtcac agcatcgtgc
      841 tcgtcgatcg caccgtcatc tacgacatcg tcgaggcgat gggcgattcc gccgaagcga
      901 tcgcgtcgta cgcgacggcg atcgatctat cgtggctgcc aacgccgtat ccgagcgcgg
      961 tgacgaagac gctgctggta tcggcggatc gcgacatcgt gcccgctcag ctgagcgagc
     1021 tcgccaccag gtacaaggcc gttgcgggtg actgggagtc cggcgcgatc gcgtacacgt
     1081 gcgcgcgcaa caagcagcgc gtgctcatcc tgcgcggcat ctccgatctc gtgcacaccg
     1141 agggcggcga agcctacggc aacatgccgg cgttcgaaga aggcgcgcgc atcgtgatgc
     1201 agcgactact tcgcgagctg ccggcgtggc tcgatcgaat gccttaggtg cggggcttgc
     1261 gctgcacgga ccaggttccg gcctgaagct ggccggtcga gctgagcctg ctctcgaacg
     1321 ttccgtagat cgagtcgtcg ttgagcgtgc cgaggaacga ggtctcgact tcacacgagc
     1381 actgcggatc cgtgtacggc gcgatcgtgc ccttgacctc gttgcgactg accttgacga
     1441 actcgatctt gagcggcttc gggcccatca tcacggtgcc ttccgcgatg tgactgccga
     1501 cctcgagcga gaagcttacc tggcccgtgc gcccactgtc gtctcccttg tactcaccct
     1561 gccactcacc cgcgacgcga gcgatatcga gatccgagcc ctgcatcgtg acttccttct
     1621 ggctgccagc gcaggcgacg acgaacaata gcgggaacat caacttcatg acgacatcgt
     1681 aatcgtcgac gaggaatcag cgtgagcgcg aaaaaaatga aagatccgta gcgcgcgctg
     1741 cactcatttt acgtgagcgt tgcgtgagct cggagtcgac gaaagttcgg tagcgcgcgc
     1801 tgcactcatt ttaggtgagc gcggaatcga cgaaagatcg gtagcgagcg ctgcacttcg
     1861 tgcagatggc agcggggtat gctcacggaa tgggattctc ctttgcgtcg gtggttctga
     1921 gctatttcct gatcgcaggc ggcaccttct tcacgatgct gctcgcactg cgacttggtc
     1981 ttcatagcga atacctcggc tacatcatcc tggccgccgg cggcttcctc ggcggcatgg
     2041 tggcagcacg cgcgagcaaa ggctcgacga tcatcgagcc ggccatcggt gcggtgctgc
     2101 tgctcgcgtc gttcatcggc gtcggtctcg cggtgtcagg caaagatgtg agcgtcgtgg
     2161 tgcttcccgg cacgatgaaa gcgatcgggc tcacagcgct tgcatcggca ggcggcggca
     2221 tcggcggcgc gttcatgacc gagaagatgt tcggcgacga gaatgcgtcg ggggcatcgt
     2281 gggtgctgtt cgtggcactc gcggcagttg gtgccggcat catcggtacg ttgtttggcg
     2341 gcgttctggc caagggcgac gccggaaaca cgatcttcgg tctaatcgcg gtctgcacgc
     2401 tgttcatcgg tattgcaacc ggcgcatcgg cgacctcccg cccgctcggc gcgtcatttc
     2461 taggcggcgc aatcggtgtc ggcggtttct tcttcctcgc gatttacctg ctcctcagca
     2521 tgttttcgga caagagccat ggcgcaggcg acatcccgag cgtggtgtac gccggcatcg
     2581 cggtggtcgc cgtcggcgcg ggcatcgtga cgctgatcgg cgcgctgttc ggctgggcga
     2641 tcgtcggcag caagcaaaat gcctagcgat cggtgagata gagcagcagc gcgatcgcga
     2701 gcaccgccac gatcggtgcg atcacgatga gcagcgcgac gcgcttggat ccaggcggct
     2761 ccggcggctc gaacgacatc tgctgtgact gtattgcgtc cgctcggctt gtccaccgac
     2821 ggaatcgtgt ttgatgccga gctggttaca gacagctcag gtcgtcacac gcacgcttcg
     2881 ccttcgcctt cgggcgcggc gcgacgtacg ggcgcgtcgg cgtgcgaatc ggccgcttca
     2941 cgcgggtcgc cgttgggagg ggctttgccg acggtggttc cacgcgggcc tcagccacgg
     3001 gcgcttcgct cggcggcgca accgccacgg gcggaaccgc cactggcgca gccgcgaccg
     3061 gcgtagccac gaccgcaacc ggtgcagctg cgctgcctgc gtcgaactgc aagagcggca
     3121 cgacgggttc gagcgccacg tcgctggtgg gcacttcggc aggagcttcg aacgacgcga
     3181 cgtcgacact cggcgcctcg accgcagcct gtgtccgctg ctgctgcgcg atcaggccag
     3241 ctgcgaatcc accgcccgcg caaacgatcg cgaccagcgc gatcgcgagc ccgacgcggc
     3301 ggcgacggcg acggcgcacg accggctgcg tctcgaggaa acgcatcggc tcgggaacgg
     3361 cctcgaccgg taccacaggc gcttcgacga caggcgcagg ctcgagcact ggcacatcga
     3421 catcgacatc gacgtcaggt gcggcctcga ccaccggcgc ggcgtcgatg acaggcactg
     3481 catcgatgac gggcgccggc acgatcggcg gcggctcgac gagcggcagc ggttcgttga
     3541 tcgacggctc gtcgacgggg actgcgactg cgatcggcgc aacctcgacc ggtgccgcga
     3601 gtccgtgtgc ggcaagcgcg aagtgattca cgacgtcgcg ctcggtgagc tcgggatcga
     3661 cggcctgtgg agcagacgac tcttcgtctg gaatctcgta cggcggctcg aacacgtcgg
     3721 ccgcgccgtc catccccgct ggcaatctcg gcaccggacc cgaaccgagg accgtgccca
     3781 ggcgcggacg ccccggcttg gtttcggcct ccggctccga cgcagccgat tcggcctcga
     3841 tcggctccgg cgccgccgga acgggctcgg ggatggcggg ctcagcagcg agcgattccg
     3901 gcggaacaag cgtgatcggc tccggcaccg cgtcggccac gggttccgga tccttcatgg
     3961 cgggtcgcga tccgcgagct acgaccggcg cgagcggcgc gaccggcgcg accggcgcaa
     4021 gcggcgtgac cggcgcgagc ggcgcgagcg gcttcacctc ggcccgcggc ttctcctgct
     4081 gcggcgttgg ctcgggttgc gcatcccagc ccatcgtcaa cttgccgggt ttcacctccg
     4141 gctgcggcgc cgggtccacg atgtcccagc cgctcgtcag cgtgctgggc ttcttcacgg
     4201 gacgcggcat cgcgggttcc gacgcacgcg cgatctcggg ccgcgctttg atcgggctcg
     4261 gctgagcggc aggggctgcg ccgttcttcg gcgcaatccg ggctggtacc ccgacgaacg
     4321 cgttgggcga cagcttcggc ttcaccgtga tcatcggctc cactgccggc gacacgctcg
     4381 ccattgcggc ggtcgccctc gtagcggcag cggtcgccgg tcttgcagcg acagctgcca
     4441 ccggcttcgc agcgacagcg gtcgccggtt tcggagcgac ggtcgctgga atcgtgacaa
     4501 ccaccgtcgg cggtttcaca ccgccagaga ccatcggctt cgtcgcgacc cgcgggactg
     4561 ctaccaccgg tgcagctgta ttcggctcgg acgccggcga cgcgggcgca tgcggaatga
     4621 tcggcagacc gagcagcgtg tgcttcgagc tcacgatcgg tgcaccttcg atcggtgcgc
     4681 tcacgagatc gatcgctcgc tgtggcactg cttgacgatc atcgaacgcc ggggtggcga
     4741 tatcggtgcc gtcctcccag ctctccggcg ccgattcttt cgaatccttc gcgagagcgc
     4801 gcgcgatcaa catctgctcg tagaccttca cgctcgccgg cgtcaccttg cgcacgccga
     4861 tctgcatgcc ggcttgaccg aatcggttgt cacgcgtcgt ccacgcaccg agcacgacgc
     4921 cttgaccgac gagcgccgaa tcgccactcg ctagatcgat cgagaatgca gcttcgattc
     4981 cttcctgacg cggcgatggc gtcggaacga agatcgatgt ctcatcgaca aagcgatgga
     5041 acacgcgcac gagctcgtcg agcgtcttgc actgcgttcg gattcggagt ccgcgagttt
     5101 ggcgggcgct catgtctgca gcacgtcccc gatgcgctgc tatccctaat aatacgacga
     5161 gctttcgagt ccgttcggat aatcatgggg tttttccgca gtgcgaagcg ctgcgtgctc
     5221 gacggatgag atgtaggtgt cgtcgatgtc tgcgattgtc cggctgttcg ttattacgcg
     5281 gtggcgacga tgtgtgtgtt ggtagcgctt cgaccgagtc ccgcgatcgc cgcgagcacg
     5341 agatgcagct cgttccgatc gcgatcgcgc gcagcattcc gccaacgcat tttgcggcga
     5401 cgagtcgcta cgaaaacctc cgatcgcgta gcagcgcgaa cccatttttc accgtcgcgc
     5461 tcgcgatacc gatcaatacc cggagtgcac gttggtcgct gatgacatcc gtgtacgatg
     5521 ctcgtggtgc gtccgttgtc tgaagacgct gacaacgaat tctgcgagga cactggcggt
     5581 tctgagattc gcgacgccat gcgcgcgacc agtgcgttcc gcctgctcgt agtcattgac
     5641 ggcgccgcgg tgacgcacga tctacctgcg cacggccgcg tcacgcttgg tcgtgcacgc
     5701 ggcagtcacg taccgatcgc gcacgtatcg gtgtctcgcg atcacgcgtt cctcgatctc
     5761 gcaccgctcg caatcgtcga tcgcaacagt cgcaacggaa cctacgtgcg tggcgcagcg
     5821 atcgatgccg gcgtgccggt aatgatcgcc gtcgacgagc cgttccgcat cggggacgtc
     5881 atggtcatgg tgcagagccg gatcgcgctc ggctcggtat cagggatctc cgacggcgcg
     5941 ccctcgcctg tcaacctcgt tgccgagtgc gcgcgttcgg tgcgtactgg tgcagcgttc
     6001 gcattcgcgc gcatcctcgt cgatccggct gtgaccgagc gggcgtgcga agcgttgatg
     6061 ggcaccgtgc gagtctccga ccagctatcg caagcgcccg gcgagttgcg ggtgctgttc
     6121 accgatacgc ctgcaacgtc gctcggcact gttgtcgatc gtttgctcag cgcgctcgat
     6181 cgtgcgggaa tcagcgcgcg cgtcggtgct gctggctttc caatcgacgg tacgacgccc
     6241 atccagctcg ccgcacgcac gtggcagcaa ctgttacgcg gccaaggtgc gcccgatccg
     6301 atcgcgccgg tgcgtgacac gctcgcgcga ctcgccgtcg gagacctgtc gctattgctc
     6361 tgcggcgaaa ccggcgtcgg caaagaggtc tacgccgaga tcattcatcg cgcatcgctg
     6421 cgttcgcgcg gtccgttgat caaggtcaac tgtgctgcgc ttaccgagac gctgctcgag
     6481 agcgagctgt tcggtcacga gcgcggtgcg ttcacgggcg cggcctctgc gcacgcgggt
     6541 ctgatcgaag cggccgatgg cggcacgctg ttcctcgacg agatcggcga gctcccgctc
     6601 atgcttcagg cgaagctgct gcgcgttctc gaagatcgcg aagtgcggcg cgtcggcggc
     6661 acgagcggcc gacagatcga cgtgcgattc gtcgccgcaa cgaaccgatc gctcgaagcc
     6721 gaggttgccg caggtcggtt ccgtcgcgat ctgctgttcc ggatcgccgg cgcgacgatc
     6781 acgatcccac cgctgcgcca gcggcgcgac gacatcgagg cgatcgcgcg cgctgtgatc
     6841 gagcaagcgc gcactggcgg cggagcggtg ctgtcctacg atgcgatcgt ggcgcttcgc
     6901 aactggcact ggcccggcaa cgtgcgcgag cttcggaata cgatcgagcg cgcgctgctt
     6961 gtcgcgcgca acggaacgat cgtcgcgcac gatctcgggc tcgaaaccga gatcgaggtc
     7021 accggtgtcg atctgctgcg ccgccgcgag ggacaggcac cgctcggcgc gtcggtcgag
     7081 gcacacgagc gcgagcagat cctcgcagca ctcgagcact gcggcggcaa caagacgcaa
     7141 gccgcgcgca tgctagggat cgctcgcaac acgctgctcg cgcggctcga cgcgtggggc
     7201 ctgcgcgacg gcaagaagta ggtattcaaa ccttgcatgc ccttcagttg ctgcatggcg
     7261 acttgtccaa tcgctggacg atcgatcgct gcgaagcatt gcggatcacg aatctagcgc
     7321 gggagtgact gcggcacgtc tctggcaaag cgcccggaca ccctgaacgg aaggtgccaa
     7381 tgcacaagat cctcgcagct ctccctctcg tgttgttcgt ggcgtgtgtg gacgaagaac
     7441 caatcacgca agcgcccgcg atcgaagatc cgggggcgga tgcactgaac gtcacgatca
     7501 ccgacacgac agcgaccggt tcgttccagg cgagcgatag cgtggtcacc ttcggggtcg
     7561 agaccgtcag ccctgccgtc tacgacatgt gggtcgagct gcatggactc cacctgacac
     7621 tgctcgtcga tgccgaggtc ggcgtgctgg agtttgatgg ttacgcggcc gacaccggcg
     7681 cgctcacgca gatgaccgaa gcggatcgcg cgacgctcct cgagctctac aaggcactcg
     7741 acgtctacgg taatgactac gagcatgtct cgctgctccg ccgcaaggtc ggcaactggg
     7801 ccgagacctc ggacacgatg aacctcacgt tccagcacct gttcgaccgc gatcagagct
     7861 ggaccagcct ctgcggttcg tgtggccgct gggtgtcggc gagccacgac tgcaaccacg
     7921 gcggctggtg gtcggatgcg acgaccgtca acgcgctcat caagtatgag ggcgcgtggt
     7981 gcggcggcga cggcagcgcg tacggcacca cgacgagcgt gtcgtgcacc ggtgagccgg
     8041 cggggttcac gacctacgag tacactcgcg gtaactgcat gggtcgctgc ggcccgggct
     8101 gcaacagcag cgacacgcag ttcacgcagg actgcgtcaa ccacgactcg tgtgttcgcg
     8161 gcggtcacga cctcgcgagc gcgtactgcg acgatcagtt tgcgtcgtgc accgatgacg
     8221 acgtctcggc gccccactgc ggtaccggct gctagcacat gacgacacgc acgttgatcg
     8281 ttggcctggg cgccgttgcc gtatgcggcg ccctcgccct gttcatgctc gcgcgtgatg
     8341 acggcgcgaa gcctacggcg gcgagccagg ctccgggcgt cagcgttcgg caccaggctg
     8401 ctatgccacc cgccgttccg accccgacgg cggccgctac gcccacgccc gctgttgccg
     8461 gagtcgagga accgctgctt caggatgaca aagaatatcc ggtggacccg aagccggcga
     8521 cgcgcgaaca accgctgacc gaggaagagc gcgatccgcg catgaagcgc tccatcgaga
     8581 tgctcgatca ggccgtcgcg cgcacggcgg cgaagctcgc cgctgcggaa gccgccggcg
     8641 ataaggtcgc cgcgaatacg gcgcgggtgc gcatcaagcg tctgcaagaa gtccgcaagc
     8701 agcgggcgag cgagctggag gcgacgcgat gagcgccacg acgaagcttg tagcggttgt
     8761 gctcgcaatc gctgcgggat gtacagaagc gcccgccgaa acgcctggga gcgacgccgt
     8821 tcgtaactac ttcgcggcag taaccgcgaa ggactgcagt gcgctcggaa ccctctccgg
     8881 tggcaaggtg gccaagaacc tcgagcgcct cggctgcgac aagctgatcg aggg
//
LOCUS       DASUFX010000477         9566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_910385,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000477 DASUFX010000000
VERSION     DASUFX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910385"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFV99_19010"
     CDS             complement(<1..711)
                     /locus_tag="VFV99_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061469.1"
                     /translation="MGSVARADTGMPAAADMASESIAVEKSHRGVAQDYLVLPSGGEL
                     TAQMKFLTADAMLDGGGLKFTDLGLFGLSGRWSLFTKLELAASVELLPKQPSFTDEKP
                     WQSVGFSLRSPLGRQIALAASGGGGHLLGHQGKWIGEALSIEWKKPIDDEFLAFDVQL
                     GINGLGISSPDTTMSSAYLTEAAMRTTALFHEPHGHWGGWIGIGYAVPLQSTGKDPTT
                     ELKIDPQPRLDFHLGTVLS"
     gene            complement(784..1212)
                     /locus_tag="VFV99_19015"
     CDS             complement(784..1212)
                     /locus_tag="VFV99_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5061470.1"
                     /translation="MRRVCAECKAQNRIPVRHLADTGKCGKCGAVLPPHASPISITSS
                     DQFDDIVTGARVPVLVDFWAQWCGPCRQVAPEVKKAATNLAGRAIVLKVDTDVLGDLA
                     QRYQVQSIPNFAVFSGGRLVRQRAGAIDHRQLEKWAVTPQ"
     gene            1235..2044
                     /locus_tag="VFV99_19020"
     CDS             1235..2044
                     /locus_tag="VFV99_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061471.1"
                     /translation="MAKTKGKSHKGKTAAKAPAKGAKPAKATAAKAPAKAAAPKTARA
                     KPSLKPSLPLPPAPPPPPPPRDELPSPRDVQRLTRYGERFGPHKIDVRWLPLQLPVTG
                     SALAVFDPAVPKSWRVFDRPVGIGTFRAMLSIARSDDGKGHVTERLAAIVIHVGRPPI
                     ARWTVAHYKGQKKPKSADQLPRVPITSGWLALIDANGDSPGVVAVPPVTLTGIQPVEV
                     PLVDGRRALALPCGNGEYAAYWAIDEGDKPIALVVDFDVFTLKEWKARPPS"
     gene            complement(2209..3627)
                     /locus_tag="VFV99_19025"
     CDS             complement(2209..3627)
                     /locus_tag="VFV99_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061472.1"
                     /translation="MDSILGVGGMGRVYKAEHTALGRAVAIKVLHARLGGSKEAAQRF
                     QREAMASGRLDHPNIVGVSDFGTLDDGSLYLVMEALEGEPLGKRLEREKRVPWPEALT
                     IIRAVLSGLKHAHDKGVVHRDIKPDNIFLAKKDGEQVVKILDFGIAKLYAGNADDPAM
                     TRAGLTVGTPAYLSPEQAVGGAITPASDLYSTSIVLYEMLTGRAPFTDEDPLAMLRAH
                     VSMDPPAFAEVAPDLELPPAIETVIRHGLEKVSAERISNARDYANELDEAIRAAGFDP
                     MTAVPRHSSQLGIPSGPHGIPTPLPGSMFMTPPSGTATPSPYHGMGSAPTESLDQIPR
                     SRMTSIAEINEPLGKGMKTAAIVIVLVALLGAGYFMFLHGGKKPDGTTKTSIVTMPVA
                     DKETRLKAALHDLDNGKTCADRKAAIPIIVQLGGPDAIAALKKARYRMRGGVLGIGDS
                     NTNACLKSDAETAIEALGGTLK"
     gene            complement(3770..5938)
                     /locus_tag="VFV99_19030"
     CDS             complement(3770..5938)
                     /locus_tag="VFV99_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEX5061473.1"
                     /translation="MVAVPRQLFSYRPYWAQRFGVAPFLPTTRAEMDELGWDSCDVIL
                     VTGDAYIDHPSFGMALVGRLLESHGFRVGILDQPEWRNADAFKSLGRPNLFWGVTAGN
                     MDSMVNRYTSDRKVRSDDAYSPDGKAGLRPDRSVIVYAQRCREAFADVPLVIGGIEAS
                     LRRIAHYDYWSDKVRRSMLVDTRADLLVYGNAERAIVEIAHRLAAGTPVDQIRDLRGT
                     AFAGKREGYSEINSTTLDTPGRVDPKPDPYAMEAERQAAPCETGESAPQPDKLVQLKP
                     RKVDRDKQVIRMPSFEQVSADPVLYAHASRILHLEANPGNARALVQRHGNHDVWINPP
                     PVPLATAEMDALYELPFSRLPHPKHQPTKLPAYEMIKFSVTILRGCFGGCSFCSITEH
                     EGRVIQSRSEKSILSEIERIRDQTPGFHGIVSDLGGPTANMYRMACKSREIEAACRKP
                     SCVFPDICDNLGTNHDPLVALYKKARALKGIKRIFIASGVRYDLAVRSPAWIKELAQH
                     HTGGYLKIAPEHTEDGPLQHMMKPGIGAYDRFKELFDRYSQEAGKEQYLIPYFIAAHP
                     GTTDEDMLNLALWLKRNGFRPDQVQAFLPSPMASATAMYHTGLNPLRSLKAKERVHIV
                     KGLERRRLHKAFLRYHDPNNWPVLREALRRMGRDDLIGSGKHQLVPSWQPAGTGQARE
                     GKRGSRIQRGVALTQHTGLPPRPLPQQRLHPNQKRPRKSR"
     gene            complement(5994..6200)
                     /locus_tag="VFV99_19035"
     CDS             complement(5994..6200)
                     /locus_tag="VFV99_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061474.1"
                     /translation="MGEIEHAGTSKTMPRIKTSEDTAGEVAGEMHGEPTIKTEAVPGG
                     AAEEQSGQADKVANQRGMPPISRP"
     gene            complement(6330..6731)
                     /locus_tag="VFV99_19040"
     CDS             complement(6330..6731)
                     /locus_tag="VFV99_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061475.1"
                     /translation="MTSRWLVVLALVACNKPKPPPAKPPEVSICAKVADHLVSLMSGA
                     TKHPPEATDPLRRVVETRCDKDAWSAEANQCLMQLTSLADGGRCQAMMTPAQVEAFQR
                     DSEAAMQDLHGQLVEEPPTQAAPPADAATVD"
     gene            complement(6728..7363)
                     /locus_tag="VFV99_19045"
     CDS             complement(6728..7363)
                     /locus_tag="VFV99_19045"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX5061476.1"
                     /translation="MSDEDVKFAVVALSAVFFVIDPLANMPIFLTITSGDSVEQRRRT
                     AARAAFAAWLTLSIFAVAGGLIFQALGISMGAFKIAGGIMLLLMSIDMMRAQPSPTRS
                     TPEEQSESIARDDVAIMPLAIPMLAGPGAIATVMVLMSRAAWHPMRTTAVLLAVTLTC
                     VVTWLLMRSAASAEKLLPKTLLRALERVMGLLLAAVAVEFIAGGVRDMIQK"
     gene            complement(7360..7728)
                     /locus_tag="VFV99_19050"
     CDS             complement(7360..7728)
                     /locus_tag="VFV99_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061477.1"
                     /translation="MSRAVACLVLMAACWRGGETAAEEPEPQRHVRGQGATCDEARLN
                     VLDVLLHAADPELVKRADTLSQLVGERCNYSAWSSELRRCVSGAKQVDDARLCEKLAT
                     DKQRKDFEIGLELVYAKESP"
     gene            complement(7704..8582)
                     /locus_tag="VFV99_19055"
     CDS             complement(7704..8582)
                     /locus_tag="VFV99_19055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5061478.1"
                     /translation="MESLDRARLALEGLSVADAFGEQLLHCGPVARQLALEHHAAPMG
                     RQWMWTDDTAMALSIVETLEAAGEIDIDALAVRFGDRYNRESARGYGGGAHQVLNAIY
                     CGIPWYAAARLLFDGQGSMGNGGGMRSAPVGAYFCDDVAAVVEQAARSAAPTHAHPDG
                     VAGAVAIAVAAARVFAGERDPRALLEAVVEHTPPGPTRGAIRRSLTMLTDEPITVAAQ
                     LGNGSRVISSDTVPFAVWCAATHLADYAGACWACASVGGDIDTTCAMVGGIIVGAVGF
                     DGIPLEWRACREPLPA"
     gene            complement(8601..9230)
                     /locus_tag="VFV99_19060"
     CDS             complement(8601..9230)
                     /locus_tag="VFV99_19060"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX5061479.1"
                     /translation="MKILYLHGFASGPMSTKGLAFERHFAAKNIAIERMNLRVPSFEH
                     LRLSAMIDTVRTAIDDRAILIGSSLGGLTATRVAERDPRVQALILMAPAFQLVARWRE
                     QLGSEWDVWRERGFREVADYTTGKQAKIDFGFVEDVEAIDVGYPDVRVPTLILHGTAD
                     NVVPIERSRTFAAGKPHVRVVELADGHELVASLPTLLAESEAFLAEHAS"
     gene            complement(9242..>9566)
                     /locus_tag="VFV99_19065"
     CDS             complement(9242..>9566)
                     /locus_tag="VFV99_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061480.1"
                     /translation="IAIEFPDEAGDVATGKRTLVVRLGAPVAARLYAAVNIAAFVWPI
                     VAIGCGLPVRVGLAMLVPAPIALWRIAQLRDHADPSAYERLTFFSVFLLVATSAAALL
                     GWLTA"
BASE COUNT         1556 a         3201 c         3172 g         1637 t
ORIGIN      
        1 tgacagcacg gtgccgaggt ggaagtcgag gcgcggttgc ggatcgatct tcagctcggt
       61 cgtcggatct ttaccggtgc tttgcagcgg cacagcgtag ccaataccga tccaaccgcc
      121 ccagtgtccg tgcggctcgt ggaacagcgc ggtcgtgcgc atggcagctt cggtcaggta
      181 cgcgctcgac atcgtcgtgt cgggcgacga gatgccgagc ccgttgatac cgagctggac
      241 gtcgaacgcg aggaactcgt catcgatcgg tttcttccat tcgatcgaga gcgcctcgcc
      301 gatccatttg ccctgatgcc cgagcaggtg accgccaccg ccgctggcgg cgagcgcgat
      361 ctgccggccg agcgggctgc gcagcgaaaa cccgacgctc tgccacggct tctcgtcggt
      421 gaacgacggc tgcttcggca acagctcgac cgatgcggcg agctcgagct tcgtgaacag
      481 cgaccagcgt cccgacaagc cgaacagccc gaggtcggtg aacttgagac cgccgccgtc
      541 gagcatcgcg tcggcggtga ggaacttcat ctgtgcggtg agctcgccgc cacttgggag
      601 cacgaggtag tcctgcgcga cgccgcgatg tgacttctcg accgcgatcg attcggatgc
      661 catgtcggcc gcggccggca tcccggtatc ggcgcgagcg acgctaccca aggaagtgat
      721 ggcggcgagc gtccaagcta attgcctcat ggggccgtag aacgcacggc cccgggcgga
      781 gatctattga ggggtcaccg cccacttctc gagctgccgg tggtcgatcg cgccggcacg
      841 ctggcggacc agccgaccgc cgctgaacac cgcgaagttc gggatgcttt gcacctggta
      901 gcgctgggcc aggtcgccaa gcacgtcggt atcgaccttg agcacgatcg cccgaccggc
      961 caggttggtc gcggccttct tcacttcggg cgcgacctgc cgacacggcc cgcaccactg
     1021 tgcccagaag tcgacgagta ccggcacgcg cgccccagtc acgatgtcgt cgaactgatc
     1081 ggagctggtg atcgagatcg gcgatgcgtg cggcggcaac acggcgccgc acttgccgca
     1141 tttgccagtg tcggcaaggt ggcgaaccgg gatccgattt tgagctttgc actcggcaca
     1201 gacgcggcgc atgaggagat gttacggtgc gcccgtggcg aagacgaagg gcaagtcaca
     1261 caaggggaag actgcggcga aggcgccggc aaagggtgcg aaacctgcga aggcaactgc
     1321 ggcaaaggcg ccggcaaagg cggctgcgcc caaaaccgcg cgggcgaaac cgtcgctgaa
     1381 gccgtcgctc ccgctgccac cggcaccgcc gccgccgccg ccgccgcgtg acgagctccc
     1441 ctccccgcgc gacgtgcagc gcctgactcg ctacggcgaa cggttcggtc cgcacaagat
     1501 cgacgtgcgc tggttgccgt tgcagttgcc ggtgactggc agtgcactcg ctgttttcga
     1561 tccggccgtg ccgaagagct ggcgcgtgtt cgatcgcccg gtcggcatcg gtacgttccg
     1621 cgcgatgctg tcgatcgccc gcagtgacga cggcaaagga catgtgaccg agaggctcgc
     1681 cgcgatcgtg atccacgttg gccggccgcc gatcgcgcgc tggaccgtcg cgcactacaa
     1741 aggccagaag aaaccgaaga gcgccgacca gctgccgcgt gtgccgatca cgtcgggctg
     1801 gctcgcgttg atcgatgcga acggcgattc gcccggcgtc gtcgcggtgc caccggtcac
     1861 gctcaccggc atccagcccg tcgaggttcc cctcgtcgat ggtcgtcgcg cgctcgcgct
     1921 gccgtgcggc aacggcgagt atgctgccta ttgggcgatc gacgaaggcg acaagccaat
     1981 cgcgctcgtc gtcgacttcg acgtgtttac gctgaaggaa tggaaggcgc ggccgccgtc
     2041 gtagggcctc cggcctcaga cctcagacct cagtggggct tcagacttca ggcctggtac
     2101 agcacttcga tgcacgcgag ggttgggctc gccaaattga tccgatgcag cgaatgctcg
     2161 cttcgcgcgg ccctcgacac gctacctgat ctgcacggca gtcagtccct atttcagcgt
     2221 gccgccgagc gcttcgatcg cagtctcggc gtcggacttg aggcacgcgt tcgtgttgct
     2281 gtcgccgatg ccgagcacgc cgccgcgcat gcgatagcgc gccttcttca gcgcagcgat
     2341 cgcgtcggga ccgccgagct gcacgatgat cggaatcgcg gccttgcgat ccgcacacgt
     2401 cttgccgttg tcgagatcgt gcagcgcggc cttgagccgc gtctctttgt cggcgacggg
     2461 catcgtcacg atcgaggtct tcgtcgtgcc gtcgggcttc ttgccgccgt ggaggaacat
     2521 gaaatagccg gcaccgagca gcgcgacgag cacgatgacg atcgccgccg tcttcatgcc
     2581 cttgccgagc ggctcgttga tctcggcgat cgacgtcatc cgagacctgg gaatctggtc
     2641 gagcgactcc gtcggcgcgc tgcccatgcc gtgatacggc gacggcgttg ccgtgccgga
     2701 tggtggcgtc atgaacatcg agcccggcag cggtgtcggt atgccgtgcg gtcccgacgg
     2761 aataccgagc tgcgaagaat ggcgcgggac cgccgtcatc ggatcgaaac cggcggcgcg
     2821 gatcgcctcg tcgagctcgt tcgcgtagtc gcgcgcgttg ctgatccgct cggccgacac
     2881 cttctcgagc ccgtgtcgga tcacggtctc gatcgcaggc ggtagctcga gatcaggggc
     2941 gacctcggcg aacgccggcg gatccatgct gacgtgtgcg cgcagcatcg cgagcggatc
     3001 ttcgtcggtg aacggcgcgc gaccggtcag catttcgtag agcacgatgc tcgtcgagta
     3061 gagatcgctc gcgggcgtga tcgcaccacc gaccgcttgc tccggtgaca gatacgccgg
     3121 tgtgccgacg gtgaggcccg cgcgcgtcat cgccggatcg tcggcgttgc cggcgtagag
     3181 cttcgcgatg ccgaagtcga ggatcttgac gacctgttcg ccgtccttct tcgcgaggaa
     3241 gatgttgtcc ggcttgatgt cgcggtgaac gacgcctttg tcgtgcgcgt gcttgaggcc
     3301 cgagagcacc gcgcgaatga tcgtgagcgc ctccggccac gggacgcgtt tctcgcgctc
     3361 gaggcgtttg ccgagcggct cgccctcgag tgcctccatg acgaggtaga gcgagccatc
     3421 gtcgagcgtg ccgaagtcgc tgacgccgac gatgttcgga tgatcgagac gacccgacgc
     3481 catcgcctcg cgctggaagc gctgcgcggc ttctttgcta ccgccgagtc gcgcgtgaag
     3541 aaccttgatc gcgaccgcgc ggccgagcgc cgtgtgctcg gctttgtaca cgcgtcccat
     3601 gcctcccaca ccgaggatcg agtccacgcg gtagcgtcca tcgaggacac tcccgatcgg
     3661 gacgatgccg gactcatccg gcgatggcga aggggccccc attccgcgtt tcccgaggat
     3721 accgcgcgaa ttgcggctca caacagggcc ctgtgtagtc tcggatcgcc tagcgggatt
     3781 tgcgcgggcg tttctgattc gggtgcaagc gttgctgggg tagcgggcgc ggtggcagac
     3841 cggtgtgctg cgtcaatgcc acaccccgct ggattcgact cccgcgctta ccttctctcg
     3901 cttggcccgt gccagccggc tgccacgacg gcaccagctg atgcttgccg ctgccgatga
     3961 gatcgtcgcg gcccatccgg cggagcgcct cgcggagcac cggccagttg ttcgggtcgt
     4021 gatagcgcag gaacgccttg tggagccgcc tccgctcgag acccttgacg atgtgcaccc
     4081 gttccttcgc cttgagcgag cggagcggat tgagcccggt gtgatacatc gcggtcgcgc
     4141 tcgccatcgg tgacggcaag aacgcctgca cctgatcggg ccggaagccg ttgcgcttga
     4201 gccacagcgc caggttcagc atgtcctcgt cggtggtgcc gggatgcgcc gcgatgaagt
     4261 acgggatcag gtactgctcc ttgcccgctt cctgcgagta gcggtcgaac agctccttga
     4321 agcgatcgta tgcgccgatg cccggcttca tcatgtgctg cagcgggccg tcctcggtgt
     4381 gctcgggtgc gatcttgagg tacccgcccg tgtgatgctg cgcgagctcc ttgatccagg
     4441 ccggcgagcg caccgcgagg tcgtagcgca cgcccgatgc gatgaagatc cgcttgatgc
     4501 ccttgagcgc gcgcgccttt ttgtacagcg cgacgagcgg atcgtgattc gtgccgaggt
     4561 tgtcgcagat atcggggaac acacacgacg gtttgcgaca cgccgcttcg atctcgcggc
     4621 tcttgcacgc catccggtac atgttcgccg tcggcccgcc gaggtcgctg acgatgccgt
     4681 ggaagcccgg cgtctgatcg cgaatgcgtt cgatctcgct caggatcgat ttctccgagc
     4741 ggctctggat cacccgacct tcgtgctcgg tgatcgagca gaagctgcac ccgccgaagc
     4801 agccgcgcag gatcgtgacc gagaacttga tcatctcgta cgcaggcagc ttcgtcggtt
     4861 ggtgcttcgg gtgtggcagc cgcgaaaacg gcagctcgta gagcgcgtcc atctcggcgg
     4921 tcgcgagcgg cactggcggc ggattgatcc acacgtcgtg gttgccgtgc cgttgcacga
     4981 gcgcgcgcgc gttccccggg ttcgcttcga gatgcaggat gcgcgacgca tgcgcgtaca
     5041 gcactggatc cgcgctgacc tgctcgaagc tcggcatgcg gatcacttgc ttgtcgcgat
     5101 cgaccttgcg cggcttgagc tgcacgagct tgtcgggctg tggtgcggac tcgccggtct
     5161 cgcacggcgc cgcttggcgc tccgcttcca tcgcgtacgg atcgggcttc ggatcgacgc
     5221 ggccgggcgt gtcgagcgtc gtcgagttga tctcgctgta tccctcgcgc ttgccggcga
     5281 atgcagtgcc gcgcagatcg cgaatctgat cgacaggcgt cccggcggcg aggcgatgtg
     5341 cgatctcgac gatcgcgcgc tccgcattgc cgtacacgag aagatcggcg cgcgtgtcga
     5401 cgagcatcga gcggcgcacc ttgtcgctcc agtagtcgta atgcgcgatg cggcgcaggc
     5461 tcgcttcgat gccgccgatc acgagcggca cgtcggcgaa cgcttcgcgg cagcgctgcg
     5521 cgtagacgat cacgctgcga tcgggacgca acccggcctt gccgtccggc gagtacgcgt
     5581 cgtcggagcg gactttgcgg tcgctcgtgt agcgattgac catcgagtcc atgttcccgg
     5641 cggtcacgcc ccagaacaga ttcgggcggc cgagcgactt gaacgcgtcg gcgttgcgcc
     5701 actcgggctg gtcgagaatg cccacgcgga acccgtgcga ctcgagcagg cgcccgacca
     5761 gcgccatgcc gaagctcgga tgatcgatgt acgcgtcacc tgtcaccaga atgacgtcgc
     5821 agctatccca gccgagctcg tccatctcgg cgcgcgtcgt cggcagaaac ggcgccacgc
     5881 cgaatcgctg cgcccagtac gggcgatacg agaacagctg acgtggaacc gcgaccattg
     5941 gagcgccaac ggttaccacg cgtcggccat cccgccgaag aagatttatg cggtcaaggc
     6001 cgtgaaatcg ggggcattcc ccgctgattc gcgaccttgt cggcctgtcc cgattgttcc
     6061 tcggcagcgc cccccggcac cgcctcggtc ttgatcgtcg gctctccatg catctcgccc
     6121 gcgacctcgc ctgccgtgtc ttcgctcgtc ttgatcctgg gcatcgtctt ggacgtaccc
     6181 gcgtgctcga tctcccccat atccgatcac gctacgaagc gacggctgtg cgcttcaagc
     6241 gtgctcacgt gatgcgggag ttagggacca agaacgtgtc agccggctcg caaaatctac
     6301 gagccgtggt cgttttgttg caggtcaaac taatcgactg tcgccgcgtc ggccggcggc
     6361 gctgcttgcg ttggcggttc ttcgacgagc tgaccatgca ggtcctgcat agctgcctcg
     6421 ctatcgcgct gaaacgcttc gacctgcgcc ggcgtcatca tcgcctggca gcgcccgccg
     6481 tccgcgagac tcgttagctg catcaaacat tggtttgcct cggcgctcca cgcgtccttg
     6541 tcgcaccggg tctcgacgac gcggcgcagc ggatccgtcg cctcaggcgg atgcttcgtc
     6601 gcgccgctca tcagcgacac gaggtgatcg gcgaccttcg cgcagatcga gacttccggc
     6661 ggcttcgccg gtggcggctt tggcttgtta cacgcgacca gcgcaagtac gacgagccac
     6721 cgcgacgtca tttctgaatc atgtcacgga cgccgccggc gatgaactcg accgcgaccg
     6781 cagcgagcag aagtcccatc acgcgctcga gtgcgcggag cagcgtcttc ggtagtagct
     6841 tctcggcgct cgcggcgctt cgcatcagca gccaggtgac gacgcacgtg agagtcactg
     6901 cgagcaacac cgcagtcgtg cgcatcggat gccacgcggc acggctcatc agcaccatca
     6961 cggtcgcgat cgcgcccggt cccgcgagca tcggaatcgc gagcggcatg atcgcgacgt
     7021 cgtcgcgcgc gatgctttcg ctctgctcct ctggcgtcga ccgcgttggc gacggctgcg
     7081 cgcgcatcat gtcgatcgac atgagcagca gcatgatgcc gcctgcgatc ttgaacgcgc
     7141 ccatcgagat cccgagcgcc tggaagatga ggccgcccgc aaccgcgaag atcgacagcg
     7201 tcaaccacgc ggcgaacgct gcacgcgcgg cggtgcgccg gcgctgctcg acactgtcac
     7261 cgctcgtgat ggtgagaaag atcggcatgt tcgcgagcgg atcgatcacg aagaacacgg
     7321 cggacagcgc gaccaccgcg aatttcacgt cttcgtcgct cacggcgatt cctttgcgta
     7381 caccagctcg agcccgatct cgaagtcttt gcgctgcttg tcggtcgcca gcttctcgca
     7441 cagccgcgca tcgtcgacct gcttggcacc ggagacgcag cgccggagct cgctcgacca
     7501 cgcactgtag ttgcagcgct cgccgacgag ctgggacagc gtatcggctc gcttgaccag
     7561 ctcgggatcc gcggcgtgca gcagcacgtc gagcacgttg agccgggcct cgtcacaggt
     7621 ggcgccttgc ccgcgcacgt ggcgttgcgg ttctggctcc tccgccgcgg tctcgccgcc
     7681 gcgccagcac gcggccatca ggactaggca ggcaacggct ctcgacacgc ccgccattct
     7741 aacggaatcc catcgaagcc gacggcacca acgatgatgc cgccgaccat cgcgcacgtc
     7801 gtgtcgatgt cgcctccgac gcttgcacag gcccagcagg cgccggcgta gtcggcgagg
     7861 tgcgtcgccg cgcaccacac ggcgaacgga acggtatccg acgagatcac gcggctgccg
     7921 ttgccgagct gggcggcgac cgtgatcggc tcgtcggtca acatcgtgag agagcgccgg
     7981 atggcaccgc gggtcggccc cggtggcgtg tgctcgacga cggcttcgag cagcgcgcgt
     8041 ggatcacgct cgccggcgaa cacgcgcgcc gcggcgaccg cgatcgccac tgcgccggcg
     8101 acgccgtccg gatgcgcgtg agtcggtgcc gccgaacgag ctgcctgctc gacgacagct
     8161 gcaacgtcat cgcagaagta cgcgccgacc ggcgccgagc gcataccgcc accgttgccc
     8221 atcgagccct gaccgtcgaa caagagacgc gccgccgcgt accacgggat gccgcaatag
     8281 atcgcgttca acacctgatg cgcgccgccg ccatagcccc gcgcggactc gcggttgtag
     8341 cggtcgccga accgcactgc gagggcgtcg atatcgatct cgccggcggc ttcgagcgtc
     8401 tcgacgatcg acagcgccat cgcggtgtcg tcggtccaca tccactgccg gcccatcggc
     8461 gccgcgtgat gctcgagcgc gagctgacgc gcgaccggac cgcaatgcag cagctgttcg
     8521 ccgaacgcgt cggcgaccga caggccctcg agggcgaggc gagcgcggtc cagtgactcc
     8581 attatagtgt cagtgtgaca ctaagatgca tgctcggcaa ggaacgcctc ggactcggcg
     8641 agcagcgtcg ggagtgacgc gacgagctcg tggccatccg ccagctcgac gacgcgaacg
     8701 tgcggcttgc cggcggcgaa cgtccgcgag cgctcgatcg gcacgacgtt gtctgcggtg
     8761 ccgtgcagga tcagggtcgg cacgcgcacg tcgggatagc cgacgtcgat cgcctcgaca
     8821 tcctcgacga agccgaagtc gatcttcgcc tgcttgccgg tcgtgtagtc ggcaacctcg
     8881 cggaagccac gctcgcgcca cacgtcccac tcggaaccga gctgctcgcg ccagcgcgcg
     8941 acgagctgga acgccggcgc catgaggatg agggcctgca cgcgcggatc gcgctcggca
     9001 acgcgggtcg cggtgaggcc accgaggctc gagccgatca ggatcgcgcg gtcgtcgatc
     9061 gccgtgcgca cggtgtcgat catcgccgac aggcgcaggt gctcgaacga cggcacgcgc
     9121 aggttcatgc gctcgatcgc gatgttcttc gccgcgaaat gcctctcgaa cgcgaggccc
     9181 ttcgtcgaca tcggccccga cgcgaacccg tgcaggtaga ggatcttcat tcggcgctag
     9241 cctacgcggt cagccagccg agaagtgcag cagcggacgt cgcgacgagc agaaataccg
     9301 agaagaacgt aagccgttcg tacgcgctcg ggtcggcatg gtcgcgcagc tgcgcgatcc
     9361 gccagagcgc gatcggtgcc ggcaccagca tcgcgagccc gacccgtacc ggcagcccac
     9421 acccgatcgc gacgatcggc cacacgaacg cggcgatatt gaccgccgcg tagagccggg
     9481 ctgcgaccgg ggcaccgagc cggaccacga gcgtgcgctt gccggtcgcg acatcgccgg
     9541 cctcgtcggg aaactcgatc gcgatc
//
LOCUS       DASUFX010000478        12666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_631806,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000478 DASUFX010000000
VERSION     DASUFX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631806"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFV99_19070"
     CDS             <1..277
                     /locus_tag="VFV99_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061481.1"
                     /translation="VAGWNINAAFGYVHEGTNTNPGASPDGSDCNPTSSGFGCAGDGI
                     ERPPSGRQGPDPTSPLIPPDNQTESPYNQGSIKSNYVMFAVGFSKGF"
     gene            complement(274..750)
                     /locus_tag="VFV99_19075"
     CDS             complement(274..750)
                     /locus_tag="VFV99_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061482.1"
                     /translation="MPTELPPCGLYRTTKKIGEVDAGRLVYFHNHGNPGPGLYFPESW
                     AHNRARFSQNGMTVPDDFDPRALRALPAEGFYRVKAPFFCCEKECMKFEPDQLVQLGY
                     NGTGKALVFIPELAGGVIGVPERGTFVDDKALENLVLLKFPERTDDISMPRGIVVH"
     gene            812..1225
                     /locus_tag="VFV99_19080"
     CDS             812..1225
                     /locus_tag="VFV99_19080"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5061483.1"
                     /translation="MRIVSQLVARPLEDCWRAFTEYASMPQWVPGLREATLVEFDVTG
                     LPYEVRFGYAVGLTYSLHYTYDLDEHVVRWEPVDNDSERGGVRGFARFEEAEGGTRFT
                     YALEHDQGRKAAERALDNPQTLVEAFARWMHEERD"
     gene            complement(1714..2139)
                     /locus_tag="VFV99_19085"
     CDS             complement(1714..2139)
                     /locus_tag="VFV99_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061484.1"
                     /translation="MMALVGCAADDGTAEISDATMTGTVGGQTWTFAAGDTSAFLSEG
                     EDNFFATLYPTTFTACGFSEPGGNHLIVGIPKTPGEYEMSLQRNMTFVVGDSQNLIAT
                     KGRIVVDEVTPTSVKGGLVGTYDGQNEVNGRFEVTICPN"
     gene            complement(2196..2927)
                     /locus_tag="VFV99_19090"
     CDS             complement(2196..2927)
                     /locus_tag="VFV99_19090"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX5061485.1"
                     /translation="MAERRFTDLELERQLAGDLPADRAFDQQATDADRARLGELRAEH
                     EAFLRSVDVDMEVKRIQQRVARATPEKRAWWRLLVPASALAAAAAVILVFLRRGGDHP
                     QKPDDDLQVKGDEISLVIHVATESGSRRVETGDQVMPGDKIRFEVDALKPGYVAVFGI
                     DGSGQVSVYQPGNGTGPMAFDPHARLLPGAIRLDATPGDEHFYALYSATPFAFDAVMS
                     ALQENRSLPAGISKAEVVLKKKVAP"
     gene            complement(2920..3456)
                     /locus_tag="VFV99_19095"
     CDS             complement(2920..3456)
                     /locus_tag="VFV99_19095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061486.1"
                     /translation="MPPHGEGDDIADLYRRHAPALHRRCASIVGNAEEARDLVQETFA
                     RYISARGNWTDRASPFTVLYRIATNASIDRLRRRKTATEEELDPETKAGDLGTEPRRI
                     DNLHDLALLTRGLSEEELTVAVLHHLDGYTQDGIAASLDMSRKTVGKILARFEDHVKK
                     RAARLGFATNPKARASDG"
     gene            complement(3506..5350)
                     /locus_tag="VFV99_19100"
     CDS             complement(3506..5350)
                     /locus_tag="VFV99_19100"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5061487.1"
                     /translation="MRLALVVLGFLAVAHSTAFAEQRYAILIGSNPGWSSDRPLRYAE
                     NDAERMRDVLVALGGFSTDRVALLRDPDTSEVRATLRDVARTVQSSSEDTLVFVYYSG
                     HADDERLHLKGDPLTFKELHATIRSLPATLKLAVIDACKSGAVTRKGGSRVDEFAVSV
                     DSPKLSGMVILTSSGADELSQESRALAGSVFTHHLVSGLRGAADTNKDNQVTLAEAYQ
                     YAYARTRADTAVTGTPQRPSFKYEITGQGELVLAQLKATKVAQMTLPKGDTKYVVLDA
                     HEWRLIAEAQAEKDREIILALAPGSYKIKKVYADRLEVGSLVVNAGDKAGLDKLAYTS
                     APISQGIVKGSPDDLSPEDHHEWQRTQAFGLLSAGQASAALNLFDQLLRDVPDDMQAW
                     RGRARALVRLAEAYQRVNDTMHERLTLNDALKADPSLTQDPNFTLWYQRLGEIDARNQ
                     MTWEQKQKLQQQVRDNPRTEKTFGLGFDLISARGLFTMRGSLAIKHMFFPHVALDIGS
                     QGFDGGIVFAPLAKRWSPFLGIGGHVSFKKLGIDFGGGGEGMVMVNDESYTTEELYGL
                     HARAEIGAQYVGRSGFTTELGLALIGFNKADGTRKTTGWPIFHFGWVW"
     gene            5468..6070
                     /locus_tag="VFV99_19105"
     CDS             5468..6070
                     /locus_tag="VFV99_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061488.1"
                     /translation="MNSSTLQTAKALDPGKGRVLVGGGFYTSPDLNSDASSASGSDVT
                     LALPYMELGYRRGIIDKLEMGAKATIPGTAGIDAKYQFLDAGKFALATGLGVGYLKIT
                     SGSEGMESKTSVIDATVPVYASYDLAKAFAVYTAPKYVLRYASSTDSDGMSSSGIDHL
                     VGGTLGTRLGNKWGLFLEGSYLKSVTSDFNAVQVNGSIFF"
     gene            6306..6443
                     /locus_tag="VFV99_19110"
     CDS             6306..6443
                     /locus_tag="VFV99_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061489.1"
                     /translation="MARCEAWLFEGNRLVRVDEDVILGTTSETLFSVRFVPMTGEAQK
                     R"
     gene            complement(6469..8400)
                     /locus_tag="VFV99_19115"
     CDS             complement(6469..8400)
                     /locus_tag="VFV99_19115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061490.1"
                     /translation="MNHFDEACELAPDAQAEYVRKLAMDDALLARDVAELLAHDRAAG
                     STLERAADELANTQTAPLGAVTSAVVRGGDLDGVQDLEPVAELGEGGMGRVLLARQRS
                     LQREVAVKVLKDASLERADDLFAEAVLAGSLEHPNIVPVHALERDRRGRPVLVMKRVE
                     GVSWRTLIREPDHPAWPSLLEKHGDRLSAHLWILNGVCLGLHFAHSRGVLHRDVKPDN
                     VMVGAFGEVYLVDWGIAAKTGLSWSTDPAAARGVGTPGFLAPEMLDDNASPPDVRTDV
                     YLLGATLHYALTGKPRHEGETVLEQLEAARASLPRDYPTHVPDELAAICNRATHRDPA
                     RRFASAEAFREAIGDYQRHRGAQELSDAAWQRLEKLRDLLATSPHADARQVHELATES
                     LFGFRESRRVWPGNARARDGLRLCLQTQVEYEVGEENLGRAQALLDEIDDPPSELVTS
                     VGALRERLAARAAREAKLAALERAQDWRVAARYRGAFFLLACALSIGLWRFAVRDRDP
                     RPIAPELLALYMVAALGVLGALVVLFRRHLLATAVTQQVVFGMLCCIGAMAVNRAANA
                     IAAPPTAAILSADMVFAALTAALGAVTLARWFWLFVPLFCGGALVARLAPQLAGMTFA
                     STATLALAIGLFASTGRRR"
     gene            complement(8424..9032)
                     /locus_tag="VFV99_19120"
     CDS             complement(8424..9032)
                     /locus_tag="VFV99_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5061491.1"
                     /translation="MSEADPDELATLLVRARGGEPAAEQALFVRVYDELRGMARGYLR
                     GRPGHTLQPTALVHEAFLRMVRRPDGFADRNHFIAVAATAMRQILVDHARRRAADKRG
                     AGRTVVTLDDHAGAAPHAHSDAGAGDVVDLLALDQVLSRLAALSQRQARIVELRCFGG
                     LTIDEVAEVLGVSGSLAEKEWRRARAWVRRELEGAAAAGDLP"
     gene            9117..9950
                     /locus_tag="VFV99_19125"
     CDS             9117..9950
                     /locus_tag="VFV99_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061492.1"
                     /translation="MKTKLGSILLSVSLMFAGCTAGSPSEDDTHDDDTSLVTTAKQSA
                     ALFSETAALAIIAVDPLAAGVANENDAAAKAAAAASGYFAEGGCISAAIDPQAPSKLV
                     LTFDDCDGPYGFADVTGKVTIEFARAGGGLSVTLASAGDLVVDDALVNVSATVTIPDL
                     ARPRAIVVKTASRLELDDDFALSLDGEFTLSWDASLCFTLAGNLTASAGTYTFSTIVD
                     DFHRCEGECPDGSVTMTMVSTSSADTRTLQVIFDGDHTAEIIVTTPDSMHSLPVPILC
                     S"
     gene            complement(10011..10943)
                     /gene="purU"
                     /locus_tag="VFV99_19130"
     CDS             complement(10011..10943)
                     /gene="purU"
                     /locus_tag="VFV99_19130"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX5061493.1"
                     /translation="MSPLEVHDAPVMITSVRRSSLRSPRATGEMGTLLVGGPDRRGIH
                     ASLAQLLHDHGVHIVHSAQHLDTGGGLFFERVRFDLSEMTSDRATLEAEIRSVCHNFG
                     MDYRLYGDRKKRVAILVSKQEHCLYDLLVRHRAGDLPPCEIAMIISNHDNAADCAAHF
                     GVPFVHLPVTAETKPQQEAQMMSLLRQHSIELIVLARYMQILSPGFVERYATRIINVH
                     HSFLPAFIGASPYRQAYERGVKLIGATSHYVTSHLDQGPILEQEVVRCSHRDSVEDLV
                     RKGRDLEKRVLAAAIRWHLEDRILVHANKTVVFE"
     gene            complement(11000..>12666)
                     /locus_tag="VFV99_19135"
     CDS             complement(11000..>12666)
                     /locus_tag="VFV99_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061494.1"
                     /translation="QPVTVGRYLLHRKIARGGMATIHIARLMGDVGFSRIVAAKRMHP
                     ELAEDREFVSMFLDEARIASKVHHRNVVPVLDVVTYGEEVVLVQEYVHGAPLSLLLRA
                     ACEAKAHVPIPVAAALACQVLAGLHAAHETLDEIGTPLNIVHRDVSPQNIMVATDGSA
                     RLLDFGVAKASLGAHVTRKGTFKGKLGYSSPEQIRGEANRQSDIYSLGVVLWELLVGH
                     RLHGNTHGEAQLIAQIMGGHLPTVTEALDEEKSWIGSYRWGQLECLEPIIRKALDVNY
                     RRRWQSAAEMEEVIAAAVPLASAADIAAWLRAVGKDFLEARENMIAEEEASWRRTLAN
                     TEEAVKLPTEAVPVIVPIQQSTGVATEPEGLRPITHTLLQRGPTAVIAGIIVVLSLMV
                     AFFMRDLEKTKAPASPPAAAYEPPQPQRPAAAAITHSATQRAAPTPSPARASVTPVAA
                     DPPRAPSDVRGVSMELPDEREELLEDQPSNEKTVDRPRSVSQKRTATNKPPARPKPAV
                     RPAPTRPTAPAHTPAPSPPTKSTATVDCSTPFYFEGSKKIFKPGCL"
BASE COUNT         2098 a         4138 c         4193 g         2237 t
ORIGIN      
        1 ggtcgcgggc tggaacatca acgccgcgtt tggctacgtg cacgaaggca cgaacacgaa
       61 tccgggcgcg agccccgacg gcagcgactg caatccgacg tcgtcgggct tcggctgcgc
      121 aggtgatggg atcgagcgtc cgccgtcggg ccgtcaagga cccgatccga cgagtccgtt
      181 gattccgccc gacaaccaga ccgagagccc gtacaaccag ggttcgatca aatcgaacta
      241 cgtgatgttc gcggtcggat tctcgaaggg cttctaatgg acgacgatgc cgcgcggcat
      301 cgagatatcg tcggtgcgct cgggaaactt tagcagcacg aggttctcga gcgctttgtc
      361 gtcgacgaac gtgccgcgct ccggcacgcc gatcacaccg ccggcgagct cggggatgaa
      421 cacgagcgcc ttgccggtgc cgttgtagcc gagctgcacg agctgatcgg gctcgaactt
      481 catgcactcc ttctcgcagc agaagaacgg cgccttcacg cgatagaagc cctcggcggg
      541 aagcgcacga agtgcgcgcg gatcgaagtc gtccggaacg gtcatgccgt tctgcgagaa
      601 ccgagcgcgg ttgtgcgccc agctctcggg gaaatagagg ccggggccgg gattgccgtg
      661 gttgtggaag tagacgaggc ggccggcgtc gacctcgccg atcttcttcg tggtgcgata
      721 caggccgcag ggcgggagct cggtgggcac ggcgacatca tatgcgacgg cgttcgcagc
      781 gacgagtcgc cccgatgcga taggctgagg catgcggatc gtgagccagc tggtggcgcg
      841 tcctctcgag gattgctggc gggcgtttac cgagtacgca tcgatgccgc agtgggtgcc
      901 cggcttgcgc gaagcgacgc tcgtcgagtt cgacgttacc ggtctgccgt acgaggtgcg
      961 cttcggctac gcggtcggac tcacgtactc gctgcactac acctacgatc tcgacgagca
     1021 cgtcgtgcgc tgggagccgg tcgacaacga cagcgagcgc ggcggcgtgc gtggcttcgc
     1081 gcgcttcgag gaagccgagg gcggcacgcg cttcacgtat gcgctcgagc acgatcaggg
     1141 acgcaaggcc gccgagcgcg cgctcgataa tccgcagacg ctcgtcgaag cgttcgcgcg
     1201 ctggatgcac gaagagcgcg actagtacct cgctgccgtg aagctgaacg tgcgtggcgc
     1261 gagctacgct aggcgcgagc gcaaaacgcc gtcgagatga tctcagcgtc gcctccacga
     1321 gcgcagttcc cagctggaac gccaagttgc caagcacgcc aagaacgcca aggccaaaga
     1381 tcgatccgac cagaggactc gacggattcc atggccccag cactcatagc tgaggctcgc
     1441 ccagcgcggg ccttgcgcct cgatggctga gccggttacc gcaagcccgt tgaggtcgga
     1501 agaagactcc gccgtaggtg tcttaccttg gcgttcttgg cgttcttggc gttcttggtg
     1561 tctagtcgtt ccatcttcgg gcggcattcg gccggccgac gaatcggttg ccgacgaggc
     1621 acagcggcgt tgtcgccggc gatcgttgtc gatcgtgtgc tgctgcgttc tacgcgtcga
     1681 tcggtcagcg cggtcgtctc ctgcgcgcgc cgatcagttc gggcagatcg tgacttcgaa
     1741 gcggccgttg acttcgttct ggccgtcgta ggtgccgacg aggccacctt tgacggaggt
     1801 cggcgtgact tcgtcgacca cgatgcggcc cttggtcgcg atcaggttct ggctatcgcc
     1861 gacgacgaac gtcatgttgc gctgcaggct catctcgtat tcgccggggg tcttcgggat
     1921 gccgacgatg aggtgattgc cgcccggctc cgagaacccg cacgccgtga acgtcgtcgg
     1981 gtagagcgtc gcgaagaagt tgtcttcgcc ctcggagagg aacgcgctgg tgtcgccggc
     2041 cgcgaacgtc caggtctggc cgccaacggt gccagtcatg gtcgcgtcgc tgatctctgc
     2101 ggttccgtcg tcggccgcgc agccgacgag cgccatcatg gaaacgacaa atagggtggt
     2161 cagtgtcttc atggcgcgtc agacgcgccg tgcgcctaag gtgcgacttt tttcttcagc
     2221 accacttcgg ccttgctgat gccggccgga agcgagcgat tttcctgaag cgcagacatc
     2281 acggcgtcga acgcgaacgg tgttgccgag tacagcgcgt agaagtgctc gtcgccggga
     2341 gtcgcgtcga gtcggatcgc gccgggcaag aggcgcgcgt gcggatcgaa cgccatcggt
     2401 cctgtgccgt taccgggctg gtagaccgag acctgacccg agccgtcgat gccgaacacc
     2461 gcgacgtaac cgggtttgag tgcgtccact tcgaagcgga tcttgtcgcc gggcatgacc
     2521 tggtcgccgg tctcgacgcg gcggctgccg ctctcggtcg cgacgtgaat cacgagcgag
     2581 atctcgtcgc ccttcacttg aaggtcgtcg tcgggcttct gggggtggtc gccgccccgc
     2641 cgcaggaaca cgaggatcac tgctgccgct gctgcgagcg cgctcgcggg gacgagcaag
     2701 cgccaccacg cgcgcttttc gggagtcgcc cgtgcgacgc gctgctggat gcgcttgacc
     2761 tccatgtcga cgtcgacgct gcgcaggaac gcttcgtgct cggcgcgcag ctcgccgagg
     2821 cgcgcgcgat cggcgtcggt cgcttgctga tcgaacgcgc gatcggccgg caagtcgcca
     2881 gcgagctgac gctcgagctc gagatcggta aagcggcgct cagccatcgc tcgccctcgc
     2941 tttggggttt gtagcaaacc cgaggcgggc cgcgcgtttc ttcacgtggt cttcgaagcg
     3001 agccaggatc tttccgaccg tcttgcgcga catatctaat gacgcggcga tgccgtcctg
     3061 ggtgtaacca tcgaggtgat ggaggaccgc cacggtcagc tcctcctcgg atagacctcg
     3121 cgtgagcagt gcgagatcat gaaggttgtc gatccgccgt ggctccgttc ccagatctcc
     3181 cgccttggtc tccggatcga gctcttcttc agtagccgtc ttgcgccggc gcaggcgatc
     3241 gatcgatgcg tttgtcgcga tgcgatagag cacggtgaac ggcgatgcgc ggtccgtcca
     3301 atttccgcgt gcggagatgt agcgcgcgaa tgtctcctgc acgaggtcgc gagcctcttc
     3361 cgcattgccg acgatactcg cgcaacggcg gtgaagcgcc ggtgcgtgac gtcggtatag
     3421 gtcggcgatg tcatcgcctt ccccatgcgg aggcatctcg catgtcatgt tagacgacgc
     3481 ggcgccgaat tgtgggaact cgaagttacc aaacccagcc gaaatgaaag atcggccatc
     3541 cggtcgtctt gcgggtgccg tcggccttat tgaagccaat cagagcgagc cctagctcgg
     3601 tcgtgaaccc ggagcggccc acgtactggg cgccgatctc ggcgcgggcg tgcaatccgt
     3661 agagctcctc ggtcgtgtag ctctcgtcgt tgaccatcac catgccttcg ccgccgccgc
     3721 cgaagtcgat gccgagcttc ttgaacgaca cgtggccacc gatcccgagg aacgggctcc
     3781 atcgcttcgc gagcggcgcg aacacgatgc cgccgtcgaa gccctgcgag ccgatatcga
     3841 gcgcgacgtg cgggaagaac atgtgcttga tcgcgagcga gccacgcatc gtgaacaggc
     3901 cacgcgccga gatcagatcg aagcccaagc cgaacgtctt ctcggtgcgt gggttgtcgc
     3961 gcacctgctg ctggagcttc tgcttctgct cccacgtcat ttgattgcgc gcatcgatct
     4021 cgccgagccg ctggtaccag agcgtgaagt tcggatcctg cgtgagcgat ggatcggcct
     4081 tgagcgcgtc gttgagcgtg agccgctcgt gcatcgtgtc gttgacgcgc tgatacgcct
     4141 cggcgagccg cacgagtgca cgcgcacggc cgcgccatgc ctgcatgtca tcgggcacgt
     4201 cacgcagcag ctgatcgaac agattgagcg cggcactcgc ctggccggcg gacaacaagc
     4261 cgaacgcctg ggtgcgctgc cactcgtggt gatcctcggg tgatagatca tcggggctgc
     4321 ctttcacgat gccctgcgag atcggcgcgc tcgtgtacgc gagcttgtcg aggcccgctt
     4381 tgtcccccgc gttgacgacc aggctgccga cctcgagccg gtcggcatag accttcttga
     4441 tcttgtagct accgggcgcg agcgccagga tgatctcgcg gtccttctcg gcttgcgctt
     4501 cggcgatcag ccgccactcg tgcgcgtcga gcacgacgta cttcgtgtcg cccttcggca
     4561 acgtcatctg cgcgaccttc gtcgccttga gctgcgcgag cacgagctcg ccctgaccgg
     4621 tgatctcgta cttgaacgac ggccgctgcg gcgtgccggt cactgcggta tcggcgcgcg
     4681 tgcgcgcata cgcgtactgg tacgcctcgg cgagcgtgac ctgattgtct ttgttcgtat
     4741 cggccgcgcc gcgcagtccg gacactagat gatgcgtgaa caccgagccg gcgagcgcac
     4801 gcgactcttg cgacagctcg tccgcgccac tgctcgtcag gatcaccatg ccggacagct
     4861 tcgggctatc gacgctgacc gcgaactcgt cgacgcgcga gccgcccttg cgcgtcactg
     4921 caccggactt gcacgcatcg atcacggcga gcttgagcgt cgccggcaag cttcgaatcg
     4981 tggcgtgcag ctccttgaac gtgagcggat cgcctttgag atgcagccgc tcgtcgtcgg
     5041 cgtgacccga gtaatagacg aacacgagcg tgtcttcgct gctcgactgc accgtgcgcg
     5101 cgacatcgcg tagcgtcgcc cgcacttcgc tcgtatccgg atcacgcagc agcgcgactc
     5161 gatcggtaga gaagccgccg agtgcgacga gcacatcgcg catgcgctcc gcatcgttct
     5221 ccgcgtagcg cagcggccga tcggatgacc agccgggatt cgagccgatc aggatcgcgt
     5281 agcgctgctc ggcgaacgcc gttgaatgcg ccacagcgag gaacccgaga acgacgagag
     5341 ctaagcgcat caaaaaaaag ttcccaatgt gggacggcgg ctccgtctta ccaccacgaa
     5401 aggaatcctc catatgaaat tcgctgccgc tgctcttctc gctttctccg gacttacgac
     5461 cgcttgtgtg aactcctcga cactgcagac cgccaaggcc ctcgatcctg gcaaaggtcg
     5521 cgtcctcgtc ggcggcggct tctacaccag cccggacctc aactccgacg catcgagcgc
     5581 ctcgggttcc gacgttacgc tagcgctgcc atacatggag cttggctatc gtcgcggcat
     5641 catcgacaag ctcgagatgg gcgcgaaggc cacgattcca ggcactgccg ggatcgatgc
     5701 caagtaccag ttcctcgacg ctggcaagtt cgcgctcgcg accggccttg gcgtcggcta
     5761 cctcaagatc acgagcggta gcgaaggtat ggagtcgaag acgagcgtca tcgacgcgac
     5821 cgtgccggtc tatgccagct acgacctcgc gaaggcgttc gcagtctaca ccgcgccgaa
     5881 gtacgtgctc cgctacgcga gcagcacgga ttccgacggc atgtcgtcaa gtggtatcga
     5941 ccacctggtc ggcggcacgc tcggcacccg tctcggcaac aagtggggcc tcttcctcga
     6001 gggctcgtac ctgaagtccg tgacgtcgga cttcaacgcg gtgcaagtca acggctcgat
     6061 cttcttctaa cgcgttgtcg gaataccgag cgccccgtta cgccgaggat gtgatcgtag
     6121 tcgcgtctac cgcgatcgcg ctcgtcactc ggggcagctc ggatcgacct cggagaagga
     6181 gcaccgacgg tgccgcttct tcccagtcga catgcactgc agcactttgc ggcggcaggt
     6241 ctcgtcggcg agaggagcgc gacagcgcaa tcagtgttgg cggacagttc gcgaggtgcg
     6301 tcgacgtggc tcgctgcgag gcgtggctat tcgaaggtaa ccgtcttgtt cgcgtggacg
     6361 aggatgtgat cctcggcacg acgagcgaga cgctgttctc cgtgcgcttc gtgccgatga
     6421 ccggcgaagc gcagaagcgc tgaacggctt cgcggccgct gactgcgatc aacgcctgcg
     6481 gccagtcgac gcgaacagcc cgatcgcgag cgcgagcgta gcggtcgacg cgaaggtcat
     6541 ccccgcgagc tgcggcgcga gccgcgcaac gagcgccccc ccgcagaaca gcgggacgaa
     6601 cagccagaac cagcgcgcca gcgttactgc gccgagcgcg gccgtgagag ccgcgaagac
     6661 catgtcggcc gacaagatcg cagcggtcgg cggcgcggcg atcgcattcg ctgcgcggtt
     6721 caccgccatc gcaccgatgc agcacagcat gccgaagacc acctgctgcg tcactgcggt
     6781 cgccagcagg tgccgtcgaa acaagacgac tagcgccccg agtacgccga gcgccgcgac
     6841 catgtagagc gccagcagct cgggcgcgat cggccgcgga tctcgatcgc gcacggcgaa
     6901 ccgccatagg ccgatcgaca gcgcacacgc gagcaggaag aacgcgccgc ggtaacgcgc
     6961 agccactcgc cagtcctgcg cgcgctcgag cgcggccagc ttcgcctcgc gcgccgcgcg
     7021 ggccgccaac cgctcgcgca gtgctccgac gctcgtgacg agctcgctcg gcggatcgtc
     7081 gatctcgtcg agcaacgcct gcgcgcgccc gaggttctcc tcgccaacct cgtactccac
     7141 ttgcgtctgc aagcacaggc gcagaccgtc tctcgcgcgc gcgttgcccg gccacacacg
     7201 ccggctctcg cggaagccga acaggctctc ggtggcgagc tcgtggacct ggcgggcgtc
     7261 agcgtgtggg ctcgtggcga ggaggtcgcg aagcttttcg agccgctgcc acgcggcgtc
     7321 gcttagctcc tgcgcgccgc ggtgacgctg gtagtcgccg atcgcctcgc ggaacgcctc
     7381 ggcgctcgcg aatcgccgcg ccggatcgcg gtgggttgcg cgattgcaga tcgccgcgag
     7441 ctcgtccggg acgtgggtcg ggtagtcacg cggcagcgat gctcgcgctg cttcgagttg
     7501 ctcgagcacg gtctcgccct cgtggcgagg cttgccggtg agcgcgtagt ggagggtcgc
     7561 cccgaggagg tagacgtccg tgcgcacatc cggtggcgac gcattgtcgt ccagcatctc
     7621 gggtgcgaga aacccgggcg tgccgacgcc gcgcgccgcc gcgggatcgg tgctccacga
     7681 tagccccgtc ttggcggcga ttccccaatc cacgaggtag acctcgccga acgcgccgac
     7741 catcacgttg tcgggcttga cgtcgcgatg gaggacaccg cgactgtggg cgaagtgcag
     7801 tccgagacag acgccgttga ggatccacag gtgcgccgat agccgatcgc cgtgtttctc
     7861 gagcagcgag ggccacgccg gatgatccgg ctcgcggatc aacgtgcgcc acgacactcc
     7921 ctccacgcgc ttcatcacaa gtaccgggcg ccccctgcga tctcgctcga gcgcgtgcac
     7981 cggaacaatg ttgggatgct ccagcgagcc ggcgagcacg gcctccgcaa acaggtcgtc
     8041 ggctcgctcc aacgaggcat ccttcagcac cttgaccgcg acctcgcgct gcagggagcg
     8101 ttggcgagcg agcagcacgc gccccattcc gccttcgccg agctcggcga cgggctcgag
     8161 atcctgtacg ccgtcgaggt cgccgcctcg cacgaccgcg ctggtcaccg cgccgagcgg
     8221 tgctgtctgc gtgttagcga gctcgtcggc ggcgcgctcc agcgtgctcc ccgccgcgcg
     8281 gtcgtgcgcg aggagctcgg ccacgtcgcg cgcgaggagg gcgtcgtcca tcgcgagctt
     8341 gcgcacgtac tccgcctgcg catcaggcgc gagctcgcac gcttcgtcga aatgattcat
     8401 caggcgttcg tggcgctcga cggtcacgga aggtctcccg ctgcggcggc gccctcgagc
     8461 tcgcgacgga cccacgcacg cgcgcggcgc cactccttct ccgcgagcga tccggacacg
     8521 ccgagaactt cggcgacctc gtcgatggtg agcccgccaa aacagcgcag ctcgacgatg
     8581 cgcgcttggc gctgactgag cgcggcgagc cgcgacagca cctggtcgag cgcgagcagg
     8641 tctacgacgt cgcccgcgcc ggcgtcgctg tgtgcatgtg gcgcggcacc ggcgtgatcg
     8701 tcgagggtga cgaccgtgcg cccggcaccg cgcttgtctg cggcacgccg acgcgcgtga
     8761 tcgacgagga tctgccgcat cgcggtcgcg gccacagcaa tgaagtggtt gcggtcggca
     8821 aagccatcgg gccggcggac catgcggaga aatgcctcgt gcacgagcgc ggtcggctgc
     8881 agcgtatggc ccggcctgcc gcggaggtag ccgcgcgcca taccgcgcag ctcatcgtac
     8941 acgcgaacga acagcgcctg ctccgccgcc ggttcgccgc cccgcgcccg tacgagcagc
     9001 gtcgcgagct catccgggtc cgcctcgctc atgcagctaa gtataggcac cgacaggagc
     9061 cttcgggtgt gaggactttc ggttcggatt gcgtttagca gggaaaggag actcgaatga
     9121 aaacgaaact tggctcgatt ctactgtcgg tgtccctcat gtttgccggc tgcacggccg
     9181 ggtcccccag cgaggacgac acccacgacg acgacacctc gctcgtgacc acggcgaagc
     9241 agtctgccgc gctgttcagc gagaccgcgg cgctcgcgat catcgcagtc gatccactcg
     9301 ccgcaggtgt tgcgaacgag aacgacgccg cggccaaggc tgccgctgcc gcgagcggct
     9361 acttcgcgga gggcggctgc atcagcgcgg cgatcgatcc gcaggcgccg agcaagctcg
     9421 tgcttacgtt cgacgattgc gacggtccgt acggcttcgc cgatgtcacc ggcaaggtga
     9481 cgatcgagtt cgcgcgtgcc ggcggcggcc tctcggtaac actggcgtcg gcaggcgatc
     9541 tggtggtcga cgacgccctg gtcaacgtct ctgcaaccgt cacgattccc gacctcgcgc
     9601 gcccgcgcgc gatcgtcgtc aagacggcca gccgcctgga gctcgacgac gacttcgcgc
     9661 tttcgctcga tggcgaattc acgttgtcgt gggacgccag cctgtgcttc acgctggccg
     9721 ggaacctcac cgccagcgcc ggcacgtaca cgttctcgac gatcgtcgac gattttcacc
     9781 gctgtgaagg cgagtgcccg gacggctccg tcacgatgac catggtcagc acgtcgtcgg
     9841 ctgacacgcg cacgctccag gtgatcttcg atggcgatca cactgccgag atcatcgtca
     9901 ctacccctga ctcgatgcac agcctcccgg tgccgattct gtgctcgtga aacgttcgtg
     9961 gtcgctgtgg cggtaggtgc accactcgag cgccgccggc gtgcgcgccg ctactcgaag
    10021 accaccgtct tgttcgcgtg gacgaggatg cgatcctcga gatgccagcg gatcgctgcg
    10081 gcgagcacgc gcttctcgag atcgcgtccc ttgcggacga gatcctcgac actgtcgcgg
    10141 tgcgagcaac gcacgacctc ttgctcgagg atcgggcctt ggtcgagatg gctcgtcacg
    10201 tagtgactcg tcgcgccgat cagcttcacg ccgcgctcgt acgcctggcg atatggactc
    10261 gcgccgatga acgccggcaa gaacgagtga tgcacgttga tgatgcgcgt cgcgtaacgc
    10321 tcgacgaagc cgggcgacag gatctgcatg taacgcgcga gcacgatcag ctcgatagag
    10381 tgctggcgca gtagcgacat catctgcgct tcttgctgcg gtttcgtctc tgccgtgacc
    10441 ggcaggtgca cgaacggcac gccgaagtgc gcggcgcagt ctgccgcgtt gtcgtggttc
    10501 gagatgatca tcgcgatttc gcacggcggt aggtcgccgg cgcgatggcg cacgagcagg
    10561 tcgtagaggc agtgctcctg cttcgagacg aggattgcca cgcgcttctt gcgatcgccg
    10621 tacaggcgat agtccatgcc gaagttgtgg cagaccgagc gaatctccgc ttcgagcgtc
    10681 gcgcgatcgg atgtcatctc cgacagatcg aagcggacgc gctcgaagaa cagaccgcca
    10741 ccggtatcga gatgctgcgc cgagtgcacg atgtgcacgc cgtgatcatg aagcagctgc
    10801 gcgagcgacg cgtgaatgcc tctgcgatcc ggaccgccga ccaataacgt ccccatctca
    10861 cccgtggcgc gcgggctgcg cagtgacgac ctgcgtaccg acgtgatcat caccggtgcg
    10921 tcgtgcactt cgagtggtga caccgccgat tcgtcgcccc acgttgcact cttcgaacgc
    10981 ggaaggggaa cgggatttgt catagacatc ctggcttgaa gattttcttg ctgccctcga
    11041 agtagaaggg cgtgctgcag tccacggtag cagtcgactt cgtgggcggt gaaggcgctg
    11101 gtgtgtgggc aggtgccgtg ggtcgcgtcg gtgcagggcg tacggcgggc ttcggtcgcg
    11161 caggtggttt gttcgtcgcc gtgcgcttct gagacacgct gcgaggacga tcgacggtct
    11221 tctcgttcga cggttgatcc tcgagcaact cctcgcgctc gtcgggcaat tccatcgaca
    11281 caccacgcac gtcagatgga gctcgcggag gatccgctgc aaccggcgtt acgctggcgc
    11341 gcgcgggaga cggtgtcggt gccgcgcgct gcgtagcgct gtgggtgatc gccgctgccg
    11401 cagggcgttg cggttgtggc ggttcgtacg cggccgcggg tggtgatgct ggagctttcg
    11461 tcttctcgag atcgcgcatg aagaacgcga ccatcagcga gagcacgacg atgattcctg
    11521 cgatgaccgc cgtcggtcca cgctgcaaca gcgtgtgcgt gatcggtcgc aagccttcgg
    11581 gctccgtcgc gacaccggtc gactgctgga tcggaacgat caccggcacc gcttcggtcg
    11641 gcagcttgac cgcctcctcg gtattcgcga gcgtgcggcg ccagctcgct tcttcctcgg
    11701 cgatcatgtt ctcgcgcgcc tcgaggaagt ccttgccgac cgcgcgcagc cacgctgcga
    11761 tgtcggcggc cgatgcgagc ggtacggcgg cagcgatgac ttcttccatc tcggcggcgg
    11821 actgccagcg ccggcgatag ttgacgtcga gcgccttgcg aatgatcggc tccaagcact
    11881 cgagctgtcc ccaccggtag ctaccgatcc acgacttctc ctcgtcgagc gcctcggtga
    11941 ccgttggcag gtggccgccc atgatctgcg cgatcagctg cgcttcgccg tgcgtgttgc
    12001 cgtgcaagcg gtgaccgacg aggagctccc acagcacgac gccgagcgaa tagatatcgc
    12061 tttgtcggtt tgcctcgccg cgaatctgtt cgggcgagct gtagccgagc ttgcccttga
    12121 acgtgccctt gcgcgtgaca tgcgcgccga gcgatgcctt cgcgacgccg aaatcgagca
    12181 ggcgcgcgct gccatcggtc gcgaccatga tgttctgcgg cgacacgtcg cgatgaacga
    12241 tgttgagcgg agtgccgatc tcgtcgagcg tttcgtgggc ggcgtgcaga cccgcgagca
    12301 cctggcacgc gagcgccgcc gcgaccggaa tcggcacgtg cgccttcgct tcgcacgccg
    12361 cgcggagcag cagcgagagc ggcgcaccgt gcacgtactc ctgcacgagc acgacttctt
    12421 cgccgtacgt cacgacatcg agcacgggta cgacgttgcg gtgatggacc ttggacgcga
    12481 tgcgcgcctc atcgaggaac atcgagacga actcgcggtc ctcggcgagc tcgggatgca
    12541 tgcgcttggc cgctacgatc cgcgagaacc cgacgtcacc catcaaccgt gcgatatgga
    12601 tcgtagccat gccgccgcgt gcgatcttgc gatgcagcag gtagcggcca acggttaccg
    12661 gttgat
//
LOCUS       DASUFX010000479         3979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1049467, whole genome shotgun sequence.
ACCESSION   DASUFX010000479 DASUFX010000000
VERSION     DASUFX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049467"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFV99_19140"
     CDS             <1..558
                     /locus_tag="VFV99_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061495.1"
                     /translation="QLIDAIFAHVEDVKQHLQITDKATEPAIDSAKKPSDVLMTLIRV
                     NRELSRVLERPFTPSDVYRTVALASAYATRIGALADTAAYERKRKPADCYAKLEQCLE
                     LVGAALTKRGETALTERGTPSDIHPTDVYDLANLVLGEVAFLHALTAGAQPLHAFEPG
                     GIGHRLPSHVHQLSRTLEAQLSSLR"
     gene            627..1865
                     /locus_tag="VFV99_19145"
     CDS             627..1865
                     /locus_tag="VFV99_19145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5061496.1"
                     /translation="MSRNRTEWIVAAWSAITFGALLTAIMVSRPVRDSLALEGDPDQI
                     PWLFTGTFVAFTIAVPLWSSAVAKEPRRVALRSFHSIALFSLGFFALMRFGIAPVVTG
                     YAFYIWSSVYNMLVVSAFWSLLADLFDPKTASKLYGPISAGGTIGAFAGPALTRLALE
                     YVDVSYMFILIAIALELAVLGVHQVRTHGAKLRIDPLAEKPLPHDPWAGLKHLMRNRY
                     LAAIGGFVLCTSAAATFVYLAQAGIVKTSFADKNARTAFFANVDMWTQALTFAVQMLL
                     ARPLLGWLGPGLVLCVLPLAQGAGMLALAAAPTLATLVAVQVTSRTATHGLTRPAREL
                     LFTVVNRDEKYRGKHVIDTLVLRFGDFASSWLYTGLVAISASATTLSLAAIPLTLVWL
                     VLALVLGAGFRRRASAKETS"
     gene            1862..3013
                     /locus_tag="VFV99_19150"
     CDS             1862..3013
                     /locus_tag="VFV99_19150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5061497.1"
                     /translation="MIIGRRRFVFGSLAAAATAACGSKSSSSPQPGPVPKPEHEKPAP
                     KKILILGGTGFIGPHIINSAIARGHTMTMFNRGKTRPELFPDVEKLHGDRDGKLDELK
                     GHQWDAVVDTSGYVPRIVKMSAELLAPNVKQYIFISSISVYAPGAPVGADESAPVEKL
                     LDPTSEDIKGNNFRNYGALKALSEQAAETAMPGRTTAVRPGLIIGPGDMTGRFTHWPT
                     RLAEGGDVLCPGDGSTPVQYIDGRDLADWIVKLIETNALGTMNALGPETKITMKEVID
                     SVNTAAGNKANPVWVDAKFLDKHEVSGWAEMPMWIPNEGEDAGFGTLSNARAVKTGLR
                     FRPIADTAKDTLAWLATLPEDERKKYRSSGIKREKEDQVLAAWKAEKKG"
     gene            complement(3047..>3979)
                     /locus_tag="VFV99_19155"
     CDS             complement(3047..>3979)
                     /locus_tag="VFV99_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5061498.1"
                     /translation="RPGTSMAGYAREIGGPLDSAAIDRVVAWLRSQGPQAQTLGTAAS
                     TGDAKRGADIYNRECQKCHGNQTTRVNAVHLANPQFLAVASDAFIRYAVVNGRPGTLM
                     ESFATKLKTQDIDDVVAYVRTLGKPVEEAKLPPPTGKEPLVLNPKGKDPVWKKLRSDP
                     GSNDPRYVSVDEVKQALADKRRMIIIDARPPSDWMRVHVQGAVSIPYHDMKRLSEVPK
                     DVWVIAYCACPHHLSGVVVDELWKQGHKRALILDEGILEWHRRGYPVTAAPGVEPPPK
                     ETQYVPIQPMPPGTPPAPGKKAVPSPTPPAPVLQ"
BASE COUNT          646 a         1374 c         1269 g          690 t
ORIGIN      
        1 caactgatcg acgcgatctt cgcgcacgtc gaggacgtca agcagcattt gcagatcacc
       61 gacaaggcga ccgagcccgc gatcgattct gcgaagaagc cgagcgacgt gctgatgacg
      121 ctgatccgcg tgaaccgcga gctgtcgcgc gtgctcgagc gtccgttcac gccgtcggat
      181 gtctatcgca cggtcgcgct cgcctcggcc tacgcgacgc gaatcggcgc gctcgcggat
      241 accgccgcgt acgagcgcaa gcgcaagccg gccgattgct acgcgaagct cgagcagtgc
      301 ctcgagctcg tcggcgctgc gctcacgaag cgtggcgaga cggcgctgac cgagcgcggc
      361 acgccgtcag acatccaccc gaccgatgtc tacgaccttg cgaatctcgt cctcggtgag
      421 gtcgcgttcc tgcacgcgct gactgcgggc gcacagccac ttcacgcgtt cgagcccggc
      481 ggcatcggcc accggttgcc gtcgcacgtg catcagctct cgcgcacgct cgaagcgcag
      541 ctgagctcgc tccgataatc cccggcaact gcgacggcat gcgacgtctc tgcgacgagc
      601 gccgcatgcg ctaacgtcgt gggcgggtgt cgcgcaatcg taccgagtgg atcgtcgccg
      661 cgtggagcgc gatcacgttc ggtgcgctgc ttacggcgat catggtgtcg cggccggtgc
      721 gcgactcgct cgcgctcgag ggcgacccgg atcagattcc gtggctattc accggcacgt
      781 tcgtcgcgtt cacgatcgcg gtgccgctat ggagctctgc ggtcgcgaaa gaaccgcgcc
      841 gtgtcgcact gcggtcgttc cattcgatcg cgctcttctc gctcggcttc ttcgcgctga
      901 tgcgcttcgg catcgcgccc gtcgtcaccg gctacgcgtt ctacatctgg tcgagcgtct
      961 acaacatgct cgtcgtctcg gcgttctgga gcctgctcgc cgatctgttc gatccgaaga
     1021 ctgcgagcaa gctctacggt ccgatcagcg cgggcggcac gatcggcgcg ttcgccggcc
     1081 ccgccctgac tcgcttggca ctcgagtacg tcgacgtctc gtacatgttc atcctgatcg
     1141 cgatcgcact cgagctcgca gtgctcggcg tccatcaggt ccgcacgcat ggcgcgaagc
     1201 tgcgcatcga tccgctcgcc gagaagccgt tgccacacga tccgtgggcg ggtctcaagc
     1261 acctcatgcg caaccgctac ctcgccgcga tcggcggatt cgtgctgtgc acgtcggcag
     1321 ccgcgacgtt cgtctatctc gcgcaggccg gcatcgtgaa gacgtcgttc gccgacaaga
     1381 acgcgcgcac ggcgttcttc gcaaacgtcg acatgtggac gcaagcgctc acgttcgcgg
     1441 tccagatgct gctcgctcgt ccgctgctcg gttggctcgg cccgggtctc gtgctgtgcg
     1501 tgctgccgct cgcgcaaggt gccggcatgc tcgcgctcgc ggccgcgcca acgctcgcaa
     1561 cgctcgtcgc ggttcaggtc acgagccgca ccgcgacgca tggcctcacg cgtcccgcac
     1621 gcgagctgtt gttcaccgtc gtcaatcgcg acgagaagta ccgcggcaag cacgtgatcg
     1681 atacgctcgt gctgcgcttc ggcgacttcg catcgtcgtg gctctacacc ggactcgtcg
     1741 cgatcagtgc gagcgcgacg acgctctcgc tcgccgcgat tccgctcacg ctcgtctggc
     1801 tcgttctcgc actggttctc ggcgccggat ttcgccggcg cgcttctgcc aaggagacgt
     1861 catgatcatc ggtcgtcgtc ggtttgtgtt cggatcgctc gctgccgccg ccacagcggc
     1921 gtgtggctcg aagtcgtcgt cctcgccgca acccggcccg gtcccgaaac ccgagcacga
     1981 gaagcccgcg ccgaagaaga tcctgatcct cggcggcacc ggcttcatcg gtccgcacat
     2041 catcaactcc gcgatcgcgc gcggtcacac gatgacgatg ttcaaccgcg gcaagacgcg
     2101 gcccgagctg ttcccagatg tcgagaagct gcacggcgat cgcgacggca agctcgacga
     2161 gctcaaggga catcaatggg acgcggtcgt cgacacgtcg ggctacgttc cgcgcatcgt
     2221 caagatgtcg gccgagctgc tcgcgccgaa cgtcaagcag tacatcttca tctcgagcat
     2281 ctcggtctac gctccgggcg cgccggtcgg tgccgacgag agcgctcccg tcgagaaact
     2341 gctcgatccg acgtccgaag acatcaaggg caacaacttt cgcaactacg gcgcgctcaa
     2401 ggcgctctcc gaacaagcgg ccgagaccgc gatgcctgga cgcacgaccg ccgttcgccc
     2461 cggcctgatc atcggccccg gcgacatgac cggccgcttc acgcactggc cgacgcgact
     2521 cgcggagggc ggcgacgtgc tgtgtcccgg cgacggctcg acgcccgtgc agtacatcga
     2581 cggccgcgat ctcgcggact ggatcgtcaa gctgatcgag acgaacgcgc tcggcacgat
     2641 gaacgcgctc ggccccgaaa cgaagatcac gatgaaagag gtgatcgaca gcgtcaacac
     2701 cgccgccggc aacaaggcga acccagtctg ggtcgacgcg aagttcctcg acaagcacga
     2761 ggtctcgggc tgggccgaga tgccgatgtg gatcccgaac gaaggcgagg acgctggctt
     2821 cggcacgctc tcgaacgcgc gcgcggtgaa gaccggcctg cgcttccggc cgatcgccga
     2881 taccgcgaag gacacgctcg cgtggctcgc cacgttgccc gaggacgagc gcaagaagta
     2941 ccgctcgtcg ggcatcaagc gcgagaaaga agaccaggtc ctcgccgcgt ggaaagcaga
     3001 gaagaagggc tagcgcgggc tagcgcgggc tagcgcgggc tagcgcctac tgcaagaccg
     3061 gcgcaggcgg cgtcgggctc ggaactgctt tctttcccgg cgccggtggg gtccccggcg
     3121 gcatcggctg gatcggcacg tactgcgtct ccttcggcgg cggctcgaca cccggcgcag
     3181 cggtcaccgg atagccgcgg cgatgccact cgaggatgcc ctcgtcgaga atcagcgcgc
     3241 gcttgtggcc ctgcttccac agctcgtcga cgacgacacc cgataggtga tgcgggcacg
     3301 cgcaatacgc gatcacccac acgtccttcg gcacttccga gagtcgcttc atgtcgtgat
     3361 acggaatcga caccgcgccc tgcacgtgca cgcgcatcca gtcggatggc ggccgcgcat
     3421 cgatgatgat catgcggcgc ttgtcggcga gcgcctgctt cacctcgtcg accgagacgt
     3481 agcgcggatc gttactgcct ggatccgaac gcagcttctt ccacaccgga tctttgccct
     3541 tgggattgag cacgagcggt tctttgcccg ttggcggtgg cagcttcgct tcctcgacgg
     3601 gcttgccgag cgtgcgcacg tacgcgacga cgtcgtcgat gtcctgcgtc ttcagcttcg
     3661 tcgcgaacga ttccatgagc gtgcccggcc ggccgttcac gaccgcgtag cgaatgaatg
     3721 catcgctcgc gacagcgagg aactgcgggt tcgcgagatg cacggcgttg acgcgcgtcg
     3781 tctggttgcc gtgacacttc tggcactctc ggttgtagat gtcggcgccg cgcttcgcat
     3841 cgcccgtcga cgcggcggtc ccgagcgtct gtgcctgcgg cccctgcgat cgcagccacg
     3901 caacgacgcg atcgatcgcc gcgctatcga gcggaccacc gatctctcgc gcgtagccag
     3961 ccatcgacgt gccgggccg
//
LOCUS       DASUFX010000480         7579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2022642, whole genome shotgun sequence.
ACCESSION   DASUFX010000480 DASUFX010000000
VERSION     DASUFX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022642"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFV99_19160"
     CDS             <1..223
                     /locus_tag="VFV99_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061499.1"
                     /translation="KKLRVDAHVLVTIVATRTLTKTVYDGKGAYDPYTSLRDEDPNVS
                     GAQITNPGFPSVKSGGELLSAGLTLTVPL"
     gene            220..1473
                     /locus_tag="VFV99_19165"
     CDS             220..1473
                     /locus_tag="VFV99_19165"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5061500.1"
                     /translation="MKALAILAALCATAHADPLDELGLGAAATAMASSRGAVATGAEA
                     AHNNPAGIARITRAEVLAGWQYSHQRLELDDHDAGVLDAHGTSLALAIPFAIGDVHVA
                     AGTALYLPDQYLARIQLTPISEPHFVRFEQSQRIVVEPVAAVAFGNVAIGAGASLLAN
                     ARSNDLTFDVGVVSGQKQGRANVDVALPVRAAPLVGAWWRPSRFVELGATFRGELSID
                     LALDITANVLVPSVVTGDVSVTLRSVSNYSPMRAALATAVHPREDLTIAAELGFERWS
                     ALGSGVPDLRVLVALDLAPPTVSTMPPPANFKNIVTSRLGAEWQTGAWRLRAGGAYLP
                     SPVPAQTGLTSFADGARTLATLGAGVRIEPNAILLQPIDIDLGFGWQHIAHETVEKDM
                     AKVPGGAFSSGGDVFQANLTSTVRF"
     gene            1755..3089
                     /locus_tag="VFV99_19170"
     CDS             1755..3089
                     /locus_tag="VFV99_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061501.1"
                     /translation="MPDAAAPTSGTGIAPWTELGAAQVCLGEQTLGPPTSAVGGFCTD
                     ADANGAACTDDSACDSRQTCVCGRCTVAYCATASDCAAPRVCNFTEHRCDVPCSDATQ
                     CATSEQCISGACRGRCLRNADCQHGEVCDSNNVCIADDCVDDTGCLTGERCEIQRIPR
                     QVLEPGPIADFGAPIVVYLDLAVPATPTQRAIYRATSTDGVHFALDPMTPVLDDPMGA
                     RAPSPIVDGGTLYLYFEQGDGVALRVATSRDGIAFDPPTTVLSGTVRLHAPSAVHVAG
                     KVALYYQRADGTGIGLATGAPGSTLDDQGVVLAPTDVEVGSGDPGTAFWLQITRVESP
                     HAVLAGTAMHLFFSAFGQESADASKYGMTEPIPPNFSVGYAGAKATAPQMLTVWPYGP
                     VFDNVDAFLDHRDELGPAVLDAGADRFFMYYIDATEAQLGRLGVLGSGARGR"
     gene            3315..4352
                     /locus_tag="VFV99_19175"
     CDS             3315..4352
                     /locus_tag="VFV99_19175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HEX5061502.1"
                     /translation="MVATVLPRVARAPGASELTLADTDSVRLMLRGDSVIDWHKLNFQ
                     DHAEVDRFLRLNEFDPESEAEMERFEDIRSDAVDYLARAFAMAIPDEVAADLPARDLF
                     LVASSTGPHQKWACVVLKVMHIIHHINGRQALLKVSVSDEIIFREIEMKVLQVVEQLR
                     AAGAPLLEFEWSRKPRDSQITKLLAKRSTLAASIYDKLRFRLIVPQHADIIPMLATLA
                     RQLIPFNYVVPGETVNQLIDLKEVLAADEMNGHEPTEVPFNEFSGPEYRIINFVADLP
                     LRLERLLPKHEMTPELSHVVFVLTEFQVCDKVTAQRNESGASSHEAYKARQHERVRMR
                     LFRDKDDPVPD"
     gene            complement(4349..6127)
                     /locus_tag="VFV99_19180"
     CDS             complement(4349..6127)
                     /locus_tag="VFV99_19180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061503.1"
                     /translation="MKPDSGDDPTVTPDQATLPAADESRPVELQDMPGYQLGTIIGRG
                     GMGEVVTALDIQFGREVALKRMRASKPTAEMTKRFIREAKVQARLDHPAIVPVHELGT
                     DSDGNPYFTMKRLVGTTLAEAIAAGRPQQPLLRAFVDVCFAMQLAHEREIVHRDLKPS
                     NVMLGNYNDTYVIDWGVARVTSQRRTSTMFAAVVDTLSPDDGGTQTGALLGTPGYMAP
                     EQMKGDDVTPAADVFSLGAILFEILAGEPLHPSGREAITSTLAKPGDSPARRKPERAI
                     APELDAVCVAALAEDPAARPTARELAERVQRYLDGDRDLEHRRTIAAEQLAHARTALA
                     DPARRSEAGQAATRALALDPESAEAAALVAQLVLEPPKELPPELVASLDAEERDLNRQ
                     RARNAMWAFLSIFTFLPVFIFLQNIANWTNLIALYAAALVMAVLSWHNGKTGNTPVWL
                     LMLGNFALAFMFGRLSSSFILVCGLVCGQTLALSTRSNIARRPWLLVSWIAAALLVPV
                     GLEYAGLIEKTWHMTPHGILSKGAILDTVRDVDVVSLAIGQAALACVVGFFAMATTRG
                     REDAQRRAHIQAWHLQQLIPRGARRG"
     gene            complement(6124..>7579)
                     /locus_tag="VFV99_19185"
     CDS             complement(6124..>7579)
                     /locus_tag="VFV99_19185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061504.1"
                     /translation="TLAKKLTQPGPQQPMLRAFVDVCLAIQLAHSRGVVHRDLKPSNI
                     MLGDYGEVYVLDWGVARVLTDTRRQTGPAMTVSADEATTAGAVLGTPGYMSPEQVRGE
                     EAGTKADVYALGAILFEILAGEPLHPRGEAAFGSTLTRPQEAPARRAAERTIPPELDG
                     VCFDALAEDPAARPSARELADRMQAYLDGDRDVERRRALANEQIVAARQALESKEPDA
                     RATAMRRAGRALALDPESADAASLVSTLLLEAPEEGKLPPDLDDYLRIEEKIFNSSRS
                     RQAALTYASLFLFWLVIPFLGVKSWITLIAFYGLLAFCGVLAWGNSRRGHSSLPVTVV
                     TTILVVLMFTRVSGPFIVTPIVICAALMQLAATRAFNERLWLVLAWTLVAVMLPFILE
                     WVGVLNPTYAVTGGVVVSASDIFDMRHFGDEVALVAGNLAFVVAAAFVAVYLNRRRFS
                     AQRQLQIQAWHLRQLLPTAKRWQTQPKGRRTMEI"
BASE COUNT         1233 a         2589 c         2517 g         1240 t
ORIGIN      
        1 caagaagctg cgcgtcgacg cgcacgtcct cgtcacgatc gtcgcgacgc gcacgctgac
       61 gaagacggtt tacgacggca agggggccta cgatccgtac acgagcctgc gcgacgagga
      121 tccgaacgtc tcgggcgcgc agatcacgaa ccccggcttt ccgagcgtga agtcgggcgg
      181 cgagctgctg tcggcaggac tcacgctgac ggtgccacta tgaaggcgct cgcgatcctc
      241 gcggcgctct gcgcgaccgc acatgccgat ccactcgacg agctcggact cggtgccgcg
      301 gcaacggcga tggcgagctc acgcggcgcc gttgcaacgg gcgcggaagc ggcgcacaac
      361 aatcccgccg gcatcgcccg catcacccgc gccgaggtgc tcgccggctg gcagtactcg
      421 caccagcgac tcgagctcga cgaccacgac gctggcgtgc tcgacgccca tggcacgtcg
      481 ctcgcgctcg cgatcccgtt cgcgatcggt gacgtccacg tcgcggccgg tactgcgctc
      541 tatctgcccg atcaatatct cgcgcgcatc cagctcacgc cgatcagcga gccgcacttc
      601 gttcgcttcg agcaatccca gcgcatcgtc gtcgagccag tcgccgcggt cgcgtttggg
      661 aacgtcgcga tcggcgctgg ggcatcgctg ctggcaaacg cgcgctcgaa cgacctgacg
      721 ttcgacgtcg gcgtcgtcag tggccagaaa cagggtcgcg caaacgtcga tgtcgcgctg
      781 ccggtgcgcg ccgcaccgct cgtcggcgca tggtggcggc cgtctcgctt cgtcgagctc
      841 ggcgcgacat tccgcggcga gctgtcgatc gatctcgcgc tcgacatcac cgcgaacgtg
      901 ctcgtgccga gtgtcgtcac cggcgacgtc tcggtcacgc tgcgctcggt cagcaactac
      961 tcgccgatgc gtgcggcgct tgccactgcc gtgcatccgc gcgaggacct cacgatcgcg
     1021 gcggagctcg gcttcgagcg ctggagcgcg ctcggctccg gagtccccga tctgcgcgtg
     1081 ctcgtcgcgc tcgacctcgc accaccgacg gtgtcgacga tgccgccacc ggcgaacttc
     1141 aagaacatcg tgacgtcgcg gctcggtgcc gaatggcaga ccggcgcgtg gcggctgcgc
     1201 gcaggcggcg cgtacctgcc ctcgccagtc cccgcacaga ccggcctcac cagcttcgcc
     1261 gatggcgcac gtacgctcgc gacgctcggc gctggcgtgc gcatcgagcc caacgcgatc
     1321 ctgctgcagc cgatcgacat cgacctcggg ttcgggtggc agcacatcgc tcacgagact
     1381 gtcgagaagg acatggcgaa ggtgccgggc ggcgcgttct cgtcgggcgg cgacgtcttc
     1441 caagcgaacc tgacctcgac ggtgaggttc tgatgggcgg aacaaccgcc acgacgccgg
     1501 gaacccggaa gaccaaccgc caagacgccg ggaacgccaa gcccagcgaa ttttcttcgc
     1561 cttggcgtct gcgtcgcctt ggcggttcaa ctcttcggcg gcgatgtgct cggccgctac
     1621 gtcacgtgga tgagctacgc atgaagacgt ccaccgccaa gacgcgggga acgccaagtc
     1681 cagacagttt ttttcttcgg cttggcgtct gcggcgtctt ggcggttgag ctcttcggtt
     1741 gtggccttgg gcctgtgccc gatgcggccg cgccgaccag cggcaccggc atcgcgccgt
     1801 ggaccgagct cggcgccgcc caggtctgcc tgggcgaaca aacgctcggc ccgcccacct
     1861 cagccgttgg cggtttctgc acggacgctg atgcgaacgg cgcagcatgc accgacgaca
     1921 gtgcatgtga ctcccgccag acctgcgtgt gcggccgctg caccgtcgcg tactgcgcga
     1981 ccgcgtccga ttgcgcggcg cctcgcgtct gcaacttcac cgagcatcgt tgcgacgtgc
     2041 cgtgctccga cgccacgcag tgtgccacca gcgagcaatg catctccggc gcgtgccgcg
     2101 gccgctgcct gcgcaacgcg gattgccagc acggcgaagt ctgcgactcg aacaacgtct
     2161 gcatcgccga cgattgcgtc gacgacaccg gctgcctcac cggcgagcgc tgcgagatcc
     2221 agcgcatccc ccgccaggtg ctcgagccgg gaccgattgc tgacttcggt gcgccgatcg
     2281 tcgtttacct cgacctcgcg gtccccgcga ccccgactca gcgcgcgatc taccgcgcga
     2341 cgtcgacgga cggcgtccac ttcgcgctcg acccgatgac gccggtgctc gacgacccga
     2401 tgggtgcccg ggccccgagc ccgatcgtcg acggcggcac gctctacctc tacttcgagc
     2461 agggcgatgg cgtggcgctg cgcgtcgcga cctcgcgtga tggcattgcg ttcgacccgc
     2521 cgaccaccgt gctctcgggc acggtcaggc tccatgcgcc gagcgccgtc cacgtcgcgg
     2581 gcaaggtcgc gctctattac cagcgcgccg atggaaccgg gatcggcctc gcgactgggg
     2641 ctcccggttc cacactcgac gatcagggcg tcgtgctcgc gcccaccgac gtcgaggtcg
     2701 gcagcggcga tcccggtacc gcgttctggc tgcagatcac gcgcgtggag agcccgcacg
     2761 ccgtgctcgc cggcaccgca atgcacctct tcttcagcgc attcggtcag gagagcgcag
     2821 acgcgtcgaa gtacgggatg accgaaccga tcccgccgaa cttctccgtc ggctatgcgg
     2881 gtgcgaaggc gacggcaccg caaatgctaa cggtctggcc gtacggtccc gtgttcgaca
     2941 atgtcgatgc gttcctcgac caccgcgacg agctcggccc cgccgttctc gacgccggcg
     3001 ccgatcgctt cttcatgtac tacatcgacg ccacagaggc gcagctcggc cggctcggcg
     3061 ttctcgggag tggcgctagg ggaaggtgag agcgctgttt tttggactct cggcgacagg
     3121 cacgcgacga tcgcgaaaca cgcgaatgga ccgaagcgtt cactcaatga ggttgcagtc
     3181 atgatcgccg tcatcggaaa tgtgcttgcc ggaatcggtg tcgtcagctt gttcacggcc
     3241 acgcttcggc tgaccagagg caagtagttc gcttcgtcgg ttgatacgcg cgcgtaagta
     3301 gtagactgtg gcccgtggtg gccacagttt tgccgcgtgt cgcacgagcg ccgggggcga
     3361 gcgagttaac gctcgccgac acggactccg tgcgcctgat gctgcgcggc gactctgtga
     3421 tcgactggca caagctgaac ttccaggacc acgcggaagt cgaccgcttc ctgcgcctca
     3481 acgagttcga tcccgagtcc gaggcggaga tggagcgctt cgaggacatt cgcagcgacg
     3541 ctgtcgatta cctcgcgcgt gcgttcgcga tggcgattcc ggatgaggtc gccgccgacc
     3601 tgccggcacg cgatctgttc ctcgtcgcct cgagcaccgg cccgcaccag aagtgggcgt
     3661 gcgtcgtgct caaggtgatg cacatcatcc atcacatcaa cggccgccag gcactgctca
     3721 aggtctcggt ctcggacgaa atcatcttcc gcgagatcga gatgaaggtg ctgcaggtcg
     3781 tcgagcagct gcgcgccgcg ggtgccccgc tgctcgagtt cgagtggagc cgcaagccgc
     3841 gcgattcgca gatcacgaag ctgctcgcga agcggtcgac gctcgccgcg agcatctacg
     3901 acaagctgcg cttccggctg atcgtgccgc agcacgccga catcatcccg atgctcgcga
     3961 cgctcgcgcg ccagctgatc ccgttcaact atgtcgtgcc cggcgagacg gtcaatcagc
     4021 tgatcgatct caaggaagtg ctcgccgccg atgagatgaa cggtcacgaa ccgaccgagg
     4081 ttccgttcaa cgagttctct ggccccgagt accgcatcat caacttcgtc gccgacctac
     4141 cgctcaggct cgagcggctg ctcccgaagc acgagatgac gcccgagctc tcgcacgtcg
     4201 tgttcgtgct caccgagttc caggtctgcg acaaggtgac cgcgcagcgc aacgaatcgg
     4261 gcgcgtcgag ccacgaggcg tacaaggcgc gccagcacga gcgcgttcgc atgcgcctgt
     4321 tccgcgacaa agacgacccg gttccggact agccgcggcg agccccgcgc gggatcagct
     4381 gctgcagatg ccaggcctga atgtgcgcgc gccgctgcgc atcttcgcgg ccgcgcgtcg
     4441 tcgccatcgc gaaaaacccg acgacacacg cgagcgcagc ctggccgatc gcgagtgaca
     4501 cgacgtcgac gtcgcgcacg gtgtcgagga tcgcgccctt cgacaggatg ccgtgcggcg
     4561 tcatgtgcca cgtcttctcg atgagccctg cgtactcgag gccgaccggg acgagcaacg
     4621 ccgccgcgat ccagctgacg agtagccacg gcctgcgcgc gatgttcgag cgcgtcgaca
     4681 gcgcgagcgt ctggccgcag accaggccgc agacgaggat gaacgagctc gatagccggc
     4741 cgaacatgaa cgcgagcgcg aagttgccga gcatcagcag ccacaccggg gtgttgccgg
     4801 tcttgccgtt gtgccaggac agcaccgcca tcacgagcgc cgcggcgtac agcgcgatca
     4861 ggttcgtcca gttcgcgatg ttctgcagga agatgaacac gggcaggaac gtgaagatcg
     4921 agaggaacgc ccacatcgcg ttgcgcgcgc gctggcggtt gaggtcgcgc tcctcggcgt
     4981 cgagtgatgc gacgagctcg ggaggtaatt ccttcggtgg ctcgaggacc aactgggcga
     5041 ccaacgccgc cgcttcggcg gattccggat cgagcgcgag tgcacgcgtg gccgcctgcc
     5101 ctgcctcgct gcgacgcgcc ggatcggcga gcgcggtgcg ggcatgggcg agctgttcgg
     5161 cggcgatcgt acggcggtgc tcgagatcgc ggtcaccatc gaggtagcgc tgcacgcgct
     5221 cggcgagctc gcgtgcggtc ggccgcgcgg ccggatcctc ggcgagtgcg gcgacacaca
     5281 cggcgtcgag ctcgggcgcg atcgcgcgtt cgggtttgcg gcgcgcaggt gaatcgcctg
     5341 gcttcgcgag cgtgctcgtg atcgcttcgc ggccggatgg atgcagcggc tcgccggcga
     5401 ggatctcgaa caggatcgcg ccgagcgaga acacatcggc ggcgggcgtg acatcgtcgc
     5461 ccttcatctg ctcgggtgcc atgtagccgg gcgtgccgag cagcgcgccg gtctgtgtgc
     5521 cgccatcatc gggcgacaac gtgtcgacga ccgcggcgaa catcgtcgac gtgcggcgct
     5581 ggctcgtcac ccgcgcgacg ccccagtcga tcacgtaggt gtcgttgtag ttgccgagca
     5641 tcacgttcga cggcttgaga tcgcggtgca cgatttcgcg ctcgtgcgcg agctgcatcg
     5701 cgaagcacac gtcgacgaac gcgcgcaaca gcggctgttg cggccgacct gccgcgattg
     5761 cttcggcgag cgtcgtgccg accagccgct tcatcgtgaa gtacggattg ccgtcgctgt
     5821 cggtgccgag ctcgtggacc ggcacgatcg ccgggtggtc gaggcgcgcc tgaaccttcg
     5881 cctcgcggat gaagcgcttc gtcatctcgg cggtcggctt acttgcgcgc atgcgcttga
     5941 gcgcgacttc gcggccgaac tggatgtcga gcgcggtgac gacctcgccc atgccgccgc
     6001 gtccgatgat ggtgccgagc tgatacccag gcatgtcctg gagctcgacg ggtctcgact
     6061 cgtccgccgc gggcaatgtc gcttgatcgg gggtgaccgt cggatcgtcc ccactatccg
     6121 gcttcatatc tccatcgtac gccgaccttt gggctgggtc tgccagcgct tggcggtcgg
     6181 caacagctgg cgcaggtgcc acgcttggat ctgcagctgg cgctgcgcgg agaatcggcg
     6241 ccggttcaaa tagacggcga cgaacgctgc cgcgacgacg aacgcgaggt ttccggcgac
     6301 gagcgcgacc tcgtcaccga agtgacgcat gtcgaagata tcggacgccg acacgacaac
     6361 gccgccggtc accgcatagg tcgggttcag cacgccgacc cattcgagga tgaatggcag
     6421 catcaccgcg acgagcgtcc acgccagtac gagccacaat cgctcgttga acgcgcgtgt
     6481 tgctgcgagc tgcatgagcg ccgcgcagat cacgatcggc gtcacgatga acggcccgga
     6541 cacgcgggtg aacatcagca cgacgaggat cgtggtgacg acagtgaccg gcaggctcga
     6601 gtggccgcgc cgcgagttgc cccacgccaa cacgccacaa aatgcgagta gtccgtagaa
     6661 cgcgatcagt gtgatccaac tcttcacgcc gaggaacgga atcacgagcc agaacaagaa
     6721 cagcgacgcg tacgtcaatg ccgcctgccg gctgcgcgag ctgttgaaga tcttctcctc
     6781 gatccgcagg tagtcgtcga gatcgggtgg caacttgcct tcctcgggag cctccagcag
     6841 caacgtcgac accagcgatg cagcgtccgc ggattccgga tcgagcgcga gtgcacgacc
     6901 ggcgcgacgc atcgcggtcg ctcgcgcgtc aggctccttg gactcgagtg cttggcgcgc
     6961 ggcgacgatc tgctcgttcg cgagcgcacg ccggcgttcg acatcgcgat caccgtcgag
     7021 gtacgcctgc atgcgatccg cgagctcgcg cgcactcggt cgcgcggcgg gatcctcggc
     7081 gagcgcgtcg aagcacacgc catcgagctc gggcggaatc gtccgttctg ccgcgcggcg
     7141 ggccggcgct tcctgcggcc tggtcagcgt gctgccgaac gccgcttcgc cacgcggatg
     7201 caacggctcg cccgccagga tctcgaacag gatcgcaccg agcgcgtaga cgtcggcctt
     7261 cgtgcccgct tcctcgccgc gcacttgctc gggagacatg tagcccggcg tgccgagtac
     7321 cgcgcccgcg gtggtcgctt cgtctgcgct cacggtcatc gccggaccgg tctgtcgccg
     7381 cgtatcggtg agcacgcgtg cgacgcccca atcgagcacg tacacctcgc cgtaatcgcc
     7441 gagcatgatg ttcgacggct tgagatcgcg gtgcacgacg ccgcgcgagt gcgcgagctg
     7501 gatcgcgaga cacacgtcga cgaacgcgcg gagcatcggc tgctgcgggc cgggttgcgt
     7561 cagcttcttc gcgagcgtg
//
LOCUS       DASUFX010000481        13463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_910871,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000481 DASUFX010000000
VERSION     DASUFX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910871"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..823)
                     /locus_tag="VFV99_19190"
     CDS             complement(170..823)
                     /locus_tag="VFV99_19190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061505.1"
                     /translation="MKRDTDSVGALGPADEAELIERCRADERAAHDELYHRFRRQVAG
                     NLYRVLGDRTDLEDLVQEVFVIAFRGLDKFRGDARLSTWLYRICVNVALGRIRTRRRR
                     PNAIGVNDLDQASTDPSLTERPETPEKSLERQRDRELVYSALERLAPKKRVVLYLHEI
                     EGLDLKEIAYLVDSNPVTVRTRLFYARREFYRVIAGPEVEAAGFTPAAGKDPSGGDE"
     gene            1006..2742
                     /locus_tag="VFV99_19195"
     CDS             1006..2742
                     /locus_tag="VFV99_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061506.1"
                     /translation="MRQLAICVGFGLVAGCTISTGEEPPTPPSWGVPLTGGTMTVTRD
                     GNRAIVSDPDRDRVVIVDLVTERVADTIALPAHSEPGRAIDDGGGRIHVALRGSGELL
                     TITGADHIVRPICGEPRGLAWQETGDLVHVACATGELVSVPAGGGDPVRVLHLDRDLR
                     DVLVQNTGLVVTTFRTAQVIKLDDQGAIVSRITPTTTTRFDFSGESGSFNIPAVPEVA
                     WRTIALPDGSLAISHQRRLGTTLRIITGGYGGQCGGAAVESNLTIVRPDGTPFAVAPI
                     VGASLPVDLAVSPTNGDLAVVSVGHDTVFVVPSASRMTPDMSDCSGMFATPLEGFQVG
                     SPTSVAYRPNGALLIFYPEADGISILDGNGLRGLSLGDRPRNDVGRTMFHSVTESGLA
                     CASCHPEARDDGGVWTFDKLGKRRTQNLGGGILGRAPYHWGADMPSLHILVDDVFTNR
                     MGGEPATDDQVRSLAKFLDRVPAPRGVVLDPDAVARGQALFDSSETGCRSCHNGALFT
                     NNTMATVGTGMTVKVPSLIGVGGRAPYMHDGCAATLRDRFGTCGGGAAHGNTGDLSEA
                     QLGDLIAYLQSL"
     gene            complement(3052..4230)
                     /locus_tag="VFV99_19200"
     CDS             complement(3052..4230)
                     /locus_tag="VFV99_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061507.1"
                     /translation="MRTVLPLTLLLATAPAAADTFGGFSGVDTPYLVNRDRICYPLSV
                     KDGAATGAPNCEKDVTADVIAKLSIKDPIAQRGPKATFAATASGKTLTVTKKASGETV
                     VTWSAIDPIGKVVEVYASQYEDRIAVAYTTRQLGKEVTNVVAFEVVKTTGREKPSTSP
                     TTPTMPTTQTPTTPTTPAPPADPKLTKAVETARKAAKGKALAAWQAVLAIDAQHSEAE
                     YRIAALRAGAKQNAEAIDALQTLAKSTREDAIEWLVEARFDKAFTALRSDAKFRAAVG
                     LDRKAQTPYERIMGFGGQWEQTGTPCDVPEVKMTVNRDRTFKLRVKTICRGQGFEQPF
                     KGTWRVDQDGIILTLPTKGKQSTVKDEAPCVFEKQKDEDALRCTLDRDLEFLVLPTRR
                     "
     gene            4311..5393
                     /locus_tag="VFV99_19205"
     CDS             4311..5393
                     /locus_tag="VFV99_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061508.1"
                     /translation="MRSVFTRASAALDRSVVRFMERRMSPRTPRVHAADARDKLLAIT
                     RAYTTGTLTAPSAFFPAPNVPQMVMSPQGDGPVGTQVVDLSWSSEYQPFLPEARDMYL
                     ASTENMTAHARWWTSDRGRPTIVMIHGWGAGSHWMNERAFQVPYWLRHGYDVAAFVLP
                     FHGVRSATGSLNPAWPTPNPLRMNEGFGHAIYDLRALAMFLRARGASAVGVMGMSLGG
                     YTTGLWTSVAGPNDPGGVDFSVAMIPAVSFARLMWRHGEGTSAQKKAANAGITEDMLV
                     EAFSVHAPTTRPPRVPRDRLYVIAGKGDRITPPDHAEALAKHWGVEVLWFEGGHLAQV
                     GRGDAVRVVRRSLGAHGFSGRAFRGS"
     gene            5390..7297
                     /locus_tag="VFV99_19210"
     CDS             5390..7297
                     /locus_tag="VFV99_19210"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX5061509.1"
                     /translation="MTDFSKPLADALASGPLLLDGAMGSLLYERGVLHTRSYDELNLS
                     QPDLIRTIHADYVAAGAEIIETNTFGANRMALARHGLVDQGTAINRAAVELARKAAAG
                     RAYVAGAVGPTGVKFHIATAAERRLARFALAEQIDTLVLAGVDAIMLETFTSILEMEE
                     AIHVAKERGPRVPVFAMMVFDGESRSDGGLGPAEIADRMIAAGADVVGGNCGIGPSEL
                     YQVVTNMVGRGKPVIAQPNAGLPASVEGRTLYVANPEHFGVFARRMLKSGVRLVGGCC
                     GTTPDHTRAMLGAVRMLRGEKIDERGTKSSPQIQISQGPAPAAKTQLPDRSRLGARIA
                     RGEFAVSVELTAPTGLDVSKTKAAVEQLIEGGVDIVNIADGPRASARMANVAVCARLA
                     AETAVEPILHVCARDRSFLGLVAHLIGAGGLGLRNLVIITGDPPKMGDYPFSTPVYDV
                     DSVGLLRIAAGLNAGVDPAGKECEPTSFVLATGAEPAAHDRERELRRLEDKKAAGAEL
                     VMTQPVYDPRIIERFLDDAAPLGLPVMVGILPLASHRNAEFLHNEVPGMSIPAEYRER
                     MAKVGGGPAARAEGIKIAQEALGAVKHRVAGAYVMPPFNRVDSALAVLEVARDRWQPV
                     AAAAAAGSRKR"
     gene            7294..8331
                     /locus_tag="VFV99_19215"
     CDS             7294..8331
                     /locus_tag="VFV99_19215"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5061510.1"
                     /translation="MSLTHDEARALHDEVCVIDLHADTPKLMDKLGYDLAERHERPMP
                     KRVNYIGHVDLPRLREGGVAAQFFAFWTWPKYVPGAERSCTKSVLDQLDALDAAVAKH
                     PEQLMWTRTGTDIRAAKAAGKIAILGGIEGGHALEGKVEHIEMFSRRGVRYLGPLHLW
                     PNALGGTSRKPKRDDGLTGLGRDVIRECERCGVIVDLAHINRRGFFEALELTTLPLMV
                     THTGVNGVHKSWRNLDDEQLRAIANRGGCTGIIFARQFLGGASLDAVVDHIKHVIDVA
                     GEDVPALGSDFDGFVIPPEGLEDIAAMPNLTVAMSRRGIPTRVIEKILSHNVLRVLDA
                     VPAFGKLAARQ"
     gene            complement(8314..10527)
                     /locus_tag="VFV99_19220"
     CDS             complement(8314..10527)
                     /locus_tag="VFV99_19220"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5061511.1"
                     /translation="MIGDPISRIDGPLKVTGRATYESEEWSYGQPLYGFIVGSTIGHG
                     RVVNIDTWRAEQARGVRRVLTYRDATGQHPPAKGGFDRYSLAFPVMNSAEVHNYGDPV
                     ALVVAETYEQARAGASLVDVTYAPSEGYFDFVAREAHAFKPEKVNAGLPTDSIVGDFD
                     TGFASAPVKLDEVYTTPYEFSLAMEMHNALAVWNGDTVTVHLSTQIVASARNRIAATL
                     GLDPRKVKVISRFVGGGFGSKLGLHADAILAVIAARALGHPVKIMLTRQQVFHMVGHR
                     PASRQRMRLGADRDGKLIAFAHEVVMKASFGDSYIEQIATVGRPLYAAPHRRSTHRGV
                     ELHVMRSEDVRAPGEAPGLLAVECAMDELAHMLQIDPIELRIRNEPPSHPELPVPFSE
                     RRLVECMREGAKRFGWDQRPKVPASVRDGRKYVGIGMAAAIRPHFQVATSVRVALDPD
                     GTAVVQSDMTDIGTGTYTILAQTVADVLGVPIANVRVELGRSEYPPSAGSGGSFGATN
                     STTAAHRAGRALRKRVALAAVADPASPLHGLDATDATFADSQIRIGSASEPLTAILAR
                     TFPSGLDALGDVPNMWDDPNLKAHSLSTYGAHFAEVHVDSDTGEIRLTRMLGMFDTGR
                     LLNAKTARSQLIGGMIWGVSAALLEEGVLDTRFGSFINHDFASYAVPVHADIPAIDVV
                     VLDSVDPVANEIGAKGLGELGICGAGAAIANAVFNATGIRVREFPITIEKLLPRG"
     gene            complement(10524..11516)
                     /locus_tag="VFV99_19225"
     CDS             complement(10524..11516)
                     /locus_tag="VFV99_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5061512.1"
                     /translation="MKPFTYERANDPEAAIAAIAAAGADAKFISGGTNLLDLMKLEIE
                     QPAHLIDISRLPLQEIDELPTGELHIGAQARNSDVAADRYVRTRYPVLSQALLAGASP
                     QLRNMASIGGNLLQRTRCVYFYDTATPCNKRAPGEGCAALGGFNRGHAILGASESCIA
                     THPSDMAVALAVLDARIETLGIGNITRSIPIRDFHLLPETTPHIETVLLPGELITAVV
                     LPPPPRGRQLYRKVRDRASYEFALVSVAATLAITNGVIDEARVAFGGVAHKPWRALEA
                     EAVLHGRPATVDTYTAAADAALARAVGRGLNDFKIELAKRTLVATLMQAAEDRT"
     gene            complement(11513..12112)
                     /locus_tag="VFV99_19230"
     CDS             complement(11513..12112)
                     /locus_tag="VFV99_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5061513.1"
                     /translation="MARSNQGVERAREAKQVEKAAGRTVGDGTESVRFTVNRKPFRLE
                     LDPRTTLLDALREHIHLCGTKKGCDHGQCGACTVLVAGRRIGSCLTLAVMHDGDDIVT
                     IEGLGTPDAMHPMQAAFLERDGYQCGYCTSGQICSAVAMLEEIKAGWPSHVTADVAAP
                     HVELTDAELRERMSGNICRCAAYPNIVAAIRDVAEGDPS"
     gene            complement(12257..12529)
                     /locus_tag="VFV99_19235"
     CDS             complement(12257..12529)
                     /locus_tag="VFV99_19235"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX5061514.1"
                     /translation="MEMENKLDGNAAGGILQAIFPFDMTMAEVICAGCGDHNVIGAAA
                     VYKHGMGTIIRCPACDTVLIRVAEISGRYWLDFSGMRALQLPATPT"
     gene            complement(12529..13302)
                     /locus_tag="VFV99_19240"
     CDS             complement(12529..13302)
                     /locus_tag="VFV99_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX5061515.1"
                     /translation="MEGTALLGRLKWQLGEVVAVHQETARTRTITLAVPWWPGHRSGQ
                     HVDVRLTSEDGYQAERSYSIASAPDASAPAMGSRVMLTVERLADGEVSPYLVDELRVG
                     DKLELRGPIGGYFVWEAEMGGPLLLVAGGSGIVPLMAMLRHRSAVGSTVATRLLYSSR
                     SYDDVIYRDELTRLTADASGLETIYTLTRAQPSGWTGYRRRIDREMLREVAWPDERRL
                     LAFICGPTPMVEAAATSLAELGHDAARIKTERFGPTGER"
     gene            complement(13272..>13463)
                     /locus_tag="VFV99_19245"
     CDS             complement(13272..>13463)
                     /locus_tag="VFV99_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5061516.1"
                     /translation="DGQPLAPEHGGPARLLVPHLYFWKSAKWVRGLRVMGKDERGFWE
                     SFGYHNYGDPWKEQRYSGD"
BASE COUNT         2160 a         4534 c         4497 g         2272 t
ORIGIN      
        1 gcgcgctgcg cacgcagagc cgggaagctc tgcgacgcac gctgcacgcg atcgcgtgcc
       61 ttcgcacacg tgctgcagcg atcggcgtgg cgctcgatct cctgcagctc cgcgtccggg
      121 agcttgccgg cgaatgcatc cgcccagcga tgtggtgcga cgtgacgact cattcgtcac
      181 ctccgctcgg gtctttcccg gcggccggag tgaatccggc cgcctccacc tctgggcccg
      241 cgatcacgcg atagaactcg cggcgcgcgt agaacaggcg cgtacgcacc gtcaccggat
      301 tgctgtcgac gaggtacgcg atctctttga ggtcgaggcc ctcgatctcg tgcaggtaga
      361 gcacgacgcg tttcttcggc gcgagccgct cgagcgcgga gtacacgagc tcgcgatcgc
      421 gttggcgttc gaggctcttc tccggcgtct cggggcgctc ggtcaggctc ggatccgtcg
      481 acgcttgatc gagatcgttc acgccgatcg cgttcggccg acgccgccgc gtgcgaatgc
      541 gaccgagcgc gacgttgacg cagatgcggt acagccatgt cgacaatcgg gcgtcgccgc
      601 ggaacttgtc gaggccgcgg aacgcgatca cgaacacttc ttggacgagg tcttcgagat
      661 cggtgcgatc tccgagcacg cggtagaggt tgccggcgac ctggcgccgg aagcggtgat
      721 agagctcgtc gtgagcggca cgctcgtcgg cacggcagcg ctcgatcagc tcggcctcgt
      781 cggccggacc gagcgcgccg acgctgtcgg tgtcccgttt cactagacgg agcataagag
      841 ggtcaggcag ttagtgcccg cctgattacc gagcgttcac gcacatccgg tcggccaatc
      901 cgtgtggcgg cccacagatt cgccagcggc ggcggcaggt tacgtgccca catatgccag
      961 ccgttgatct gaaacgtttg ccggctcgcg gtgcactatg aatccatgag gcagctagcg
     1021 atctgcgtcg ggtttggact tgtggccggc tgcacgatct cgacgggcga agagccgccg
     1081 acgccgccgt cctggggcgt gccgctgacc ggcggcacga tgaccgtgac ccgcgacggc
     1141 aatcgcgcca tcgtctcgga tccggatcgc gatcgcgtcg tgatcgtcga cctcgtgacc
     1201 gaacgcgtcg ccgacacgat cgcactgccg gcgcacagcg aacccggacg cgcgatcgac
     1261 gatggcggcg gccgcattca cgtcgcgctg cgcggcagcg gcgagctgct cacgatcacc
     1321 ggcgccgatc acatcgtgcg cccgatctgc ggcgagccgc gcggcctcgc gtggcaggag
     1381 accggcgatc tcgtgcatgt cgcctgtgcg accggcgagc tcgtcagcgt gcccgccggc
     1441 ggcggcgatc cggtgcgcgt acttcacctc gatcgcgatc tgcgcgacgt gctcgtgcag
     1501 aacaccggcc tcgtcgtcac gacgttccgc accgcgcagg tgatcaagct cgacgatcaa
     1561 ggcgccatcg tctcgcgcat cacaccgacc acgacgacgc gcttcgactt ctcgggtgag
     1621 agcggaagct tcaacatccc cgcggtgccg gaggtcgcgt ggcgcacgat cgccctgccc
     1681 gacggctcgc tcgcgatctc gcatcaacgc cggctcggca cgacgctgcg gatcatcacc
     1741 ggcggctacg gcggtcagtg cggcggcgca gccgtcgaat cgaacctcac gatcgtccgc
     1801 cccgacggta cgccgttcgc ggtcgcgccg atcgtcggcg cctcgttgcc cgtcgacctc
     1861 gcggtcagcc cgacgaacgg cgatctcgca gtggtgtccg tcggtcacga caccgtgttc
     1921 gtcgtgccat ccgcgtcgcg gatgacgccc gacatgagcg attgcagcgg catgtttgcg
     1981 acgccgctcg aaggcttcca ggtcggctcg ccgacgtcgg tcgcgtatcg cccgaacggc
     2041 gcgctgctca tcttctaccc ggaagccgac gggatctcga tcctcgacgg caacggtctg
     2101 cgcggcctct cgctcggcga tcgcccgcgc aacgacgtcg gccgcacgat gttccacagc
     2161 gtgaccgagt cgggcctcgc gtgcgcgtcg tgccatcccg aagcgcgcga cgacggcggc
     2221 gtgtggacat tcgacaagct cggtaagcgc cgcacgcaga acctcggcgg cggcatcctc
     2281 gggcgcgcgc cgtatcactg gggggccgac atgccgtcgc tgcacatcct cgtcgacgac
     2341 gtgttcacga accggatggg cggcgaaccg gcgaccgacg atcaggtgcg gtcgctcgcg
     2401 aagttcctcg atcgcgtacc cgcgccgcgc ggcgtcgtcc tcgatcctga cgcggtcgcg
     2461 cgcggccagg cgttgttcga ttcgagcgag actggctgcc gcagctgcca caacggcgcg
     2521 ctgttcacga acaacacgat ggcgacggtc ggcacgggca tgacggtcaa ggtgccgagc
     2581 ctgatcggcg tcggcgggcg cgcgccgtac atgcacgatg gttgcgcggc gacgctgcgc
     2641 gatcgtttcg gcacgtgcgg cggtggcgca gcgcatggca ataccggcga cctcagcgag
     2701 gcgcagctcg gcgatctcat cgcgtatctg cagtcgctct agcgccgcgc gaaagtccgt
     2761 atccccgagc gcttcgcgag gcgatcgagc cggcggccgc gcaccagacc ggttcgcgca
     2821 gaagcgaatg cgtcagcgcc tgtggcgccg cagatgaaac gcaaagatgc caagcacgcc
     2881 aagggcgtct tttgatcgcg ttctgctcgc gcagtcgtac gggcagagcg cgcgtcgaga
     2941 ccagctctct gatggcgacc ttggcgacct tggcgatctt ggtgtcttgg cgttgatctc
     3001 cgaggcggcg aagccgccgt gcgccgcgaa tcgtttcgag ctgaaatggg actagcggcg
     3061 cgtcggcagc acgaggaact cgagatcgcg gtcgagcgtg cagcggagcg cgtcctcgtc
     3121 cttctgcttc tcgaacacgc acggcgcttc gtccttcacc gtgctctgct tgcccttcgt
     3181 cggcagcgtc aggatgatgc cgtcctgatc gacgcgccac gtgcccttga acggctgctc
     3241 gaagccttgg ccgcgacaga tggtcttcac acgcagcttg aacgtgcggt cgcggttcac
     3301 ggtcatcttc acctcgggga cgtcgcaggg cgtgccggtc tgctcccact gaccgccaaa
     3361 gcccatgatc cgttcgtagg gcgtctgcgc cttgcgatcg agaccgaccg cggcgcgaaa
     3421 cttcgcgtcc gagcgcagcg ccgtgaacgc cttgtcgaag cgcgcctcga cgagccactc
     3481 gatcgcatcc tcgcgcgtcg atttcgcgag cgtctgcagc gcgtcgatcg cctccgcgtt
     3541 ctgcttcgcg ccggcgcgca gtgcagcgat ccggtactcc gcctccgaat gctgggcgtc
     3601 gatcgcgagc acggcctgcc acgcggcgag cgccttgccc tttgctgctt tgcgcgcggt
     3661 ctccaccgcc ttggtcagct tcggatcggc tgggggcgcc ggcgtggtcg gtgtggtcgg
     3721 cgtctgcgtg gttggcatcg tcggcgtcgt cggtgacgtc gatggcttct cgcggccggt
     3781 cgtcttcacg acttcgaacg ccacaacatt cgtgacttcc ttgccgagct ggcgcgtcgt
     3841 gtacgcgacg gcgatgcgat cttcgtactg gctggcgtag acctcgacga ccttgccgat
     3901 cggatcgatc gcgctccacg tcaccacggt ctcgccgctc gccttcttcg tgacggtgag
     3961 cgtcttgccc gacgcggtcg ccgcgaacgt cgccttcggt ccgcgctgcg cgatcgggtc
     4021 cttgatcgag agcttcgcga tcacgtccgc ggtgacgtcc ttctcgcaat tcggggcgcc
     4081 ggtcgccgcg ccgtccttga cggagagcgg gtaacagatc cgatcgcggt tcacgaggta
     4141 cggcgtgtcg acaccggaga agccgccaaa cgtgtctgcc gcggccggag cagtggcaag
     4201 cagcagggtt agcgggagga cggtacgcat cgccatttcg acgtgatagc gcgatttcca
     4261 ttcagatgcc cacgggtgct caaacttgcg aattgaggta cggtcaatag gtgcgttccg
     4321 tctttacccg cgcgagtgcc gccctcgatc gcagcgtcgt ccggttcatg gagcgacgca
     4381 tgtcaccgcg tacaccgcgt gtgcatgcgg ccgacgcgcg cgacaagctg ctcgcgatca
     4441 cacgcgcgta caccaccggc acgttgacgg cgccgagcgc gttcttcccg gcgccgaacg
     4501 ttccgcagat ggtgatgtcg ccgcagggcg atggcccggt cggtacgcag gtcgtcgatc
     4561 tgtcgtggtc gtcggagtac cagccgtttt tgcccgaggc gcgcgacatg tatctcgcga
     4621 gcaccgagaa catgacagcg cacgcgcggt ggtggacgag cgatcgcggt cggccgacga
     4681 tcgtgatgat tcacggctgg ggtgccggca gccactggat gaacgagcgc gcgttccagg
     4741 tcccgtactg gctgcgccat ggctatgacg tcgccgcatt cgtgctgccg ttccacggcg
     4801 tgcgctcggc gaccggctcg ctcaacccgg cgtggccgac gccgaacccg ctgcgcatga
     4861 acgaaggctt cggccacgcg atctacgatc tgcgcgcgct cgcgatgttt ctccgtgcgc
     4921 gtggcgcgtc ggcggtcggc gtcatgggca tgagcctcgg tggctacacg acgggtctgt
     4981 ggacgagcgt cgcgggtccg aacgatcccg gcggcgtcga tttttcggtc gcgatgattc
     5041 cagccgtgtc gttcgcgcgg ctgatgtggc gtcacggtga aggcacgagc gcgcagaaga
     5101 aggccgcgaa tgcggggatc accgaggaca tgctcgtcga ggcattctcg gtccacgcac
     5161 cgacgacgcg ccctccccgc gtcccgcgcg accgcctcta cgtgatcgcc ggcaagggcg
     5221 atcggatcac gccgcccgac cacgcggaag cgctcgccaa gcactggggt gtcgaggttc
     5281 tgtggtttga aggcggtcat ctcgcgcaag tcgggcgcgg cgacgcggtt cgcgtggtac
     5341 gacgctcgct aggcgcgcac ggcttctccg gccgcgcctt ccgaggctca tgaccgactt
     5401 ttcgaaacct ctcgccgacg cactcgcgag cggaccgctc ctgctcgacg gcgcgatggg
     5461 gtcgctcttg tacgagcgcg gcgtcctgca cacgcgcagc tatgacgagc tcaacctgtc
     5521 gcagccggat ctgattcgca cgatccacgc cgactacgtc gccgccggcg ccgagatcat
     5581 cgagacgaac acgttcggcg ccaatcgcat ggcgctcgcg cgccacggcc tcgtcgacca
     5641 aggcaccgcg atcaatcgcg ccgcggtcga gctcgcgcgc aaagcggccg ctggtcgcgc
     5701 gtacgtcgcc ggtgcggtcg ggccaacggg cgtgaagttt cacatcgcaa cggccgccga
     5761 gcgccgtctc gcccgattcg cgctcgccga gcagatcgac acgctcgtgc tcgccggcgt
     5821 cgacgcgatc atgctcgaga cgttcacgtc gatcctcgag atggaagaag cgattcacgt
     5881 cgcgaaggaa cgcgggccac gcgtgcccgt gttcgcgatg atggtgttcg acggcgagag
     5941 ccgcagcgac ggtgggctcg gtcccgccga gatcgcggac cgcatgatcg cggcaggcgc
     6001 cgacgtcgtc ggtgggaact gcggcatcgg cccgtcggag ctgtaccagg tcgtcacgaa
     6061 catggtcggg cgcggcaagc cggtgatcgc gcaaccgaac gcaggcctgc cggcctccgt
     6121 cgagggccgc acgctgtacg tcgcaaaccc ggagcacttc ggcgtgttcg cgcgccgcat
     6181 gttgaagagc ggcgtccgtc tcgtcggtgg ttgctgcggc accacgcctg atcacacgcg
     6241 cgcgatgctc ggcgccgtgc gcatgctgcg cggcgagaag atcgacgagc gcggcacgaa
     6301 gtcgtctccg cagatccaga tctcgcaggg cccggcgccg gccgcgaaga cgcaattgcc
     6361 cgatcgcagc cgcctcggtg cgcggatcgc acgcggcgag ttcgcggtgt cggtcgagct
     6421 caccgcgccg accggtctcg atgtcagcaa gacaaaggcg gccgtcgagc agctgatcga
     6481 gggcggtgtc gacatcgtca acatcgccga cggtccacgc gcctcggccc gcatggcaaa
     6541 cgtcgccgtg tgcgcgcggc tcgcggcgga gaccgcagtg gagccgatcc ttcacgtctg
     6601 cgcgcgcgat cgcagcttcc tcggcttggt cgcgcacctg atcggcgcgg gcgggcttgg
     6661 cctgcgcaac ctcgtcatca tcaccggcga tccgccgaag atgggcgatt acccgttctc
     6721 gacgccggtc tacgacgtcg actcggtcgg tctccttcgc atcgccgccg gcctcaacgc
     6781 gggcgtcgat cccgcaggca aggaatgcga gccaacgtcg ttcgtgctcg cgaccggtgc
     6841 ggaaccggct gcgcacgatc gcgagcgcga gctgcgccgc ctcgaagaca agaaggccgc
     6901 cggtgccgag ctggtgatga ctcagccggt ctatgacccg cgcatcatcg agcgctttct
     6961 cgacgacgcc gcgccactgg gtctgccggt gatggttggc atcctgcccc tcgcatcgca
     7021 tcgcaacgcc gagttcctcc acaacgaggt ccccggcatg agcattcccg ccgagtatcg
     7081 cgagcggatg gcgaaggtcg gcggcggtcc ggcggcgcgc gccgagggca tcaagatcgc
     7141 gcaagaggcg cttggcgcgg tcaagcaccg tgtcgccggt gcgtacgtga tgccgccgtt
     7201 caaccgcgtc gactcggcgc ttgccgtgct cgaggtcgca cgcgatcgct ggcagcccgt
     7261 cgcagccgcg gcggccgctg gatcgcgcaa gcgatgagcc tcacgcacga cgaagcgcgt
     7321 gcgctgcacg acgaagtctg cgtgatcgat ctacacgccg acacgccgaa gctgatggac
     7381 aagctcggct acgaccttgc cgaacgacac gagcggccga tgcccaagcg cgtgaactac
     7441 atcggtcacg tcgatctacc gcggttgcgc gaaggcggcg tcgccgcgca gttcttcgcg
     7501 ttctggacgt ggcctaagta cgtacccggt gccgaacgga gctgcacgaa gtccgtgctc
     7561 gatcaactcg acgcgctcga cgcggcggtc gcgaaacacc ccgagcaact gatgtggacg
     7621 cgcaccggta cggatattcg cgccgcgaag gccgccggca agatcgcgat cctcggtggg
     7681 atcgaaggcg gccacgcgct cgaaggcaag gtcgagcaca tcgagatgtt ctcgcggcgc
     7741 ggcgtgcgct acctcggccc gctgcatctg tggccaaacg cgctcggcgg tacgtcgcgt
     7801 aaaccgaaac gcgacgacgg gctcaccggg ctcggccgtg acgtgattcg cgagtgcgaa
     7861 cgctgcggtg tgatcgtcga tctcgcccac atcaatcggc gcggcttctt cgaggcgctc
     7921 gagctgacga cgctgccgct catggtgacg cacactggtg tcaatggcgt gcacaagagc
     7981 tggcgcaacc tcgacgacga acagctgcgt gcaatcgcga atcgcggcgg ctgcaccggc
     8041 atcatcttcg cgcggcagtt tctcggcggc gcgtcgctcg acgcggtggt cgatcacatc
     8101 aagcacgtga tcgacgtcgc cggcgaggac gtgcccgcgc tcggcagcga cttcgacggc
     8161 ttcgtgatcc cgcccgaggg cctcgaggac atcgccgcga tgccaaacct gaccgtcgcg
     8221 atgtcgcggc gcggaattcc gacgcgcgtg atcgagaaga tcctcagcca caacgtgctg
     8281 cgtgtcctcg acgcagtgcc ggcgtttggc aagctagccg cgcggcaata gcttctcgat
     8341 cgtgatcgga aactcgcgca cgcgaatgcc ggttgcgttg aacaccgcgt tcgcgatcgc
     8401 agcgcccgca ccacagatgc ccagctcgcc gagacccttc gcaccgatct cgttggcgac
     8461 cgggtcgacg ctgtcgagca cgacgacatc gatcgctggg atgtcggcgt ggaccggcac
     8521 tgcgtagctc gcgaagtcgt gattgatgaa cgagccaaac cgcgtgtcga gcacgccttc
     8581 ttcgagaagc gcggcactga cgccccagat catcccgccg atcagctgcg agcgcgctgt
     8641 tttcgcgttg aggagccgcc cggtgtcgaa catgccgagc atgcgggtta gccggatctc
     8701 gccggtgtcg ctgtcgacgt ggacctcggc gaagtgcgcg ccgtaggtgc tgagcgaatg
     8761 cgccttcaga ttcgggtcgt cccacatgtt cgggacatcc ccgagcgcgt cgagcccgga
     8821 tgggaacgtg cgggcgagga tcgcggtcag cggctccgag gcactgccga tgcgaatctg
     8881 cgaatcggcg aacgtcgcgt cggtcgcgtc gagaccatgc agcggtgatg cgggatcggc
     8941 gaccgccgct agggccacgc gcttgcggag tgcacgaccc gctctgtggg cggcagtcgt
     9001 cgaattcgtc gcaccgaacg agccgccgga tccggcactg ggcgggtact ccgaacgacc
     9061 gagctcgacg cgcacgttcg cgatcggcac gccgagcacg tcggctaccg tctgcgcgag
     9121 gatcgtatac gtgccggtgc cgatgtccgt catgtccgat tggacgaccg cggtaccgtc
     9181 gggatcgagc gcgacgcgca ccgacgtcgc gacctggaag tgcgggcgaa tcgcggcggc
     9241 cataccgatg ccgacgtact tgcggccatc acgaacgctg gccggcacct tcgggcgctg
     9301 atcccagcca aaccgtttcg cgccctcgcg catacactcg acgagcctgc gctcgctgaa
     9361 cggaaccgga agttcgggat ggctcggcgg ttcgttccgg atccgcagct cgatcggatc
     9421 gatctgcagc atgtgagcga gctcgtccat cgcgcactcg accgcgagca acccgggcgc
     9481 ctcacccggc gcgcgcacat cctccgagcg catgacgtga agctcgaccc cacgatgggt
     9541 gctccggcga tgcggcgccg cgtatagcgg acgcccgacc gtcgcgatct gctcgatgta
     9601 gctgtcgccg aagctcgcct tcatcacgac ctcgtgagcg aacgcgatca gcttgccgtc
     9661 gcggtcagca cccaggcgca tgcgctgtcg cgatgcggga cgatgaccga ccatgtgaaa
     9721 cacttgctgg cgcgtcagca tgatcttgac cggatgaccg agcgcgcgcg ccgcgatgac
     9781 cgcgaggatc gcgtcagcgt gaagcccgag cttcgagcca aagccgccgc cgacgaaacg
     9841 cgagatgact ttgaccttgc gcgggtcgag cccgagcgtc gctgcgatgc ggttacgcgc
     9901 cgacgcgacg atctgggtgc tgaggtggac ggtgacggta tcgccgttcc agaccgcgag
     9961 ggcgttgtgc atctccatcg cgagtgagaa ctcgtagggt gtcgtgtaaa cctcgtcgag
    10021 cttgaccggc gcgctggcga agccggtgtc gaagtcgccg acgatcgaat cggtcggcaa
    10081 tcccgcgttc accttctcgg gtttgaatgc atgcgcctcg cgcgccacga aatcgaagta
    10141 gccctcgctc ggcgcgtatg tgacatcgac gagcgacgcg ccagcgcgcg cttgctcgta
    10201 cgtttcggcg acgacgagcg cgaccggatc gccgtagttg tggacctcgg cgctattcat
    10261 caccgggaac gcgagcgagt aacgatcgaa gccacccttg gccggcgggt gctggccggt
    10321 cgcatcccga tacgtcagca cccgcctcac gccgcgcgct tgctcggcgc gccaggtatc
    10381 gatgttgacg acgcggccgt ggccgatggt ggaaccgacg atgaatccgt agagcggctg
    10441 gccgtagctc cactcctccg attcgtacgt cgcgcggccg gtgaccttga gtggaccgtc
    10501 gatgcgagag atgggatcgc cgatcatgtg cggtcctccg cggcttgcat gagcgttgca
    10561 acgagtgttc gcttcgcgag ctcgatcttg aagtcgttca ggccgcggcc aaccgcgcgc
    10621 gcaagcgcgg catccgcagc agcggtgtac gtgtcgacag tggcgggccg accatgcagc
    10681 acggcttccg cctcgagcgc tcgccacggc ttgtgcgcga cgccgccgaa cgcgacgcgc
    10741 gcctcgtcga tcacaccgtt cgtgatcgcg agcgtcgctg cgaccgaaac cagcgcgaac
    10801 tcgtacgacg cgcggtcacg gaccttgcga tacagctggc gaccccgtgg tggcggcggc
    10861 aacacgaccg cggtgatcag ctcgccgggc agcagcaccg tctcgatgtg gggcgtggtc
    10921 tccggtagca gatggaagtc gcggatcgga atactgcgcg tgatgttgcc gatgccgagc
    10981 gtctcgatcc gcgcgtcgag caccgccagc gcgaccgcca tatccgacgg gtgcgtcgcg
    11041 atgcacgact cgctcgcacc gagaatcgcg tgaccgcgat tgaacccgcc aagcgcagca
    11101 cagccctcgc cgggcgcgcg cttgttgcac ggcgttgcgg tgtcatagaa gtacacgcag
    11161 cgcgtgcgct gcagcaggtt gcccccaatc gaagccatgt tgcgtagctg cggcgatgcg
    11221 ccggcgagga gcgcttgcga caacaccgga tagcgcgtgc gaacgtagcg atcggcggcg
    11281 acatccgagt tgcgcgcctg cgcgccgatg tgaagctctc cggtcgggag ctcgtcgatt
    11341 tcctgcagcg gcaagcgact gatgtcgatg agatgagccg gctgctcgat ctcgagcttc
    11401 atcaggtcga gcaggttcgt accgccgctg atgaacttcg cgtccgcgcc tgccgcagca
    11461 atcgctgcga tcgccgcctc gggatcgttc gcgcgctcgt acgtgaacgg cttcatgacg
    11521 gatctccttc ggcgacatcg cgaatcgccg cgacaatgtt gggatacgca gcgcagcggc
    11581 agatgtttcc gctcatgcgc tcgcgcagct ctgcgtcggt gagctcgacg tgcggcgccg
    11641 cgacgtcggc ggtgacgtga ctgggccagc ccgctttgat ctcctccagc atcgcgaccg
    11701 cggagcagat ctggcccgag gtgcagtagc cgcactgata gccgtcgcgc tcgaggaagg
    11761 cggcctgcat cggatgcatc gcatcgggcg taccgagccc ttcgatcgtg acgatgtcat
    11821 cgccatcgtg catgaccgcc aaggtgaggc acgagccgat acgccgaccc gcgacgagca
    11881 ccgtacaggc gccgcactgg ccgtggtcgc agcccttctt ggtcccacac aggtggatgt
    11941 gctcgcgaag cgcgtcgagc agcgtggtac gtggatcgag ctcgagccgg aacggtttgc
    12001 ggttgaccgt gaagcgcacc gactcggtac cgtcacctac ggtacgccct gccgcttttt
    12061 cgacctgctt cgcttcgcgt gcgcgttcga cgccttgatt cgatctcgcc atgccgcgcg
    12121 acgcgtgcaa gcgagggacc gcggcacagg cgcataccgt aacgacggct cgtgcggagt
    12181 gcgagacgaa cgcccggtgt gcattccgcg ctcatcgcgt ttcgcgagta cggttttgac
    12241 cgcgtcgcga tcctcctcag gtcggcgtgg caggcagctg cagggcccgc atgccactga
    12301 agtccaacca gtagcgtccg ctgatctcgg cgactcggat aaggaccgta tcgcacgcgg
    12361 gacatcggat gatcgtaccc atgccgtgct tgtagaccgc ggccgctccg atcacattgt
    12421 gatcgccgca gcccgcgcag atgacctcgg ccatggtcat gtcgaacgga aaaatcgcct
    12481 gcagaatgcc gcccgcggcg ttgccatcca gtttgttctc catctccatc atcgctctcc
    12541 tgtcggcccg aaccgttcgg ttttgatccg cgcggcgtcg tgacccaact cggccaggct
    12601 cgtggcggca gcctcgacca tcggagtcgg cccgcagatg aatgccagca gacgtcgctc
    12661 gtccggccag gccacctcgc gcagcatctc ccgatcgatg cgacggcgat aaccggtcca
    12721 gccggagggc tgcgctcgag tcagcgtgta gatcgtttcg aggccgctcg cgtcggcagt
    12781 cagacgagtc agctcatcgc gatagatcac gtcgtcgtag ctgcgcgagg aatagagtaa
    12841 gcgcgtggcc accgtgcttc ccaccgcact gcgatgacga agcatcgcca tgagaggcac
    12901 gatgccggag ccgcccgcga ccagcagcag cggcccgccc atctccgctt cccacacgaa
    12961 gtagccgccg atagggccgc gaagctcgag cttgtcgccg acgcgcagct catcaaccag
    13021 atacggagac acctcgccat cggcgagacg ttcgaccgta agcatcacgc gggagcccat
    13081 cgccggagct gatgcgtccg gagctgatgc gatcgagtag ctgcgctcgg cctgatagcc
    13141 gtcctcggag gtgagccgaa cgtcgacgtg ctgccctgaa cggtggcccg gccaccatgg
    13201 gacggcgagc gtgatggtgc gcgtgcgcgc agtctcttga tgtacagcga caacctcgcc
    13261 gagctgccac ttcagtcgcc cgagtagcgc tgttccttcc atggatcacc gtagttgtga
    13321 tagccgaacg actcccagaa ccctcgttcg tccttgccca tgacacgcaa gcctcgcacc
    13381 cactttgcgc tcttccagaa gtagaggtga ggaacgagca agcgtgccgg accgccatgt
    13441 tctggagcga gcggttgacc gtc
//
LOCUS       DASUFX010000482        25113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_632309,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000482 DASUFX010000000
VERSION     DASUFX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25113
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632309"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFV99_19250"
     CDS             <1..864
                     /locus_tag="VFV99_19250"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX5061517.1"
                     /translation="TKETLHRYTQIVGKIQLGRTPIENHFWNVALRMTSRGLATSALE
                     YEGRTFDIEIDLVEHAVHIRTSDGGRAKVELRPLAVADFYREIQEALDRLGIDVPIWD
                     HPVELKTDVIPFSQDRMHAEYDREYANRFFHVLSSATEVLEEFRSRFTGKCSGVGFYW
                     GTFDVSVAMYSGRAVENAPTESRIEREAYSHELIEVGFWPGDAVYQQPAFFAMQWPMP
                     ESYAHAHLEPADASWLEPSRCFVLPYESGRNGDYTARVLDFCQSAYETGANMAGWDPG
                     LERNHEMWADH"
     gene            complement(861..1718)
                     /locus_tag="VFV99_19255"
     CDS             complement(861..1718)
                     /locus_tag="VFV99_19255"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEX5061518.1"
                     /translation="MNRLAPFAVFAFTGCIADVGSVDVPDEQSEVTTIGATTPDSFAC
                     FRPESASSVNGGYSCQGSPNLSLSTLCREGASYDVIVAPTAAEMTMGIVAPHGGGIEL
                     GTDRIARQLAADLHLPSYVLVAHPTDTCLDKYGGPTRSARALHVTSTHFNDSRAESLM
                     RSVNRGIAIHGHGGPNKICVGGITPALRTAFKHYYDTYTAQYSASGAIAVDAPGDSDC
                     AGITGTASANISNRTGGHLGVQLEMSRTFRNELVASIDGTDHRLWISFRNAVRAVCRL
                     NVDGVRGCF"
     gene            1944..2480
                     /locus_tag="VFV99_19260"
     CDS             1944..2480
                     /locus_tag="VFV99_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061519.1"
                     /translation="MGSRWLQRVTLAVAAGASIATTQLPQEWHADASAVLDAVVFDTQ
                     RVTASYAIHAEIAGTGKYTDLDGTLTATISATTAGTFDAQSQLRLTLRSTTNPDVQSV
                     SEVTLPNIDPRVAAELPAWTHCMTPPCAEDFELTVELIGTGAPPLNITGNVGVRSLGT
                     DNKLEATTQTTIIASPLP"
     gene            2477..2977
                     /locus_tag="VFV99_19265"
     CDS             2477..2977
                     /locus_tag="VFV99_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061520.1"
                     /translation="MIATRTLMHPISLGAIALLVINDHVLKHVAPGVITGKLSDFAGL
                     VFFPLLLAAAAEHAGIRRGIQTVIVAAIATAAAFAAIKLSASAAEIYRNGLGVLQWPF
                     RAARAMLLGESVPSVGRVAHVVDATDLVALVALGVPIALVHRAMRFPSTVPAKCAGST
                     ESVYPQ"
     gene            3024..3797
                     /locus_tag="VFV99_19270"
     CDS             3024..3797
                     /locus_tag="VFV99_19270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5061521.1"
                     /translation="MRAIAHGRPRVLRRHRAPGCLFQNGLINCLTVKDVREEGTDVAR
                     IRGVLLVDGHPIVRDGLAAWIDSQAGLEVVGHAASADEAQAIKRQLNPDLVVVGVALR
                     NSTGIELTRRLVARRDVCILGLSGLPGPGLAMQGARMLLAGATGFALKSQPSKEILDA
                     LRMTLERKCYITPAIEAEVKTLLHGPSTVAASQLTVREREIWKLVVKGYSTKEIGQAL
                     FISPRTVEVHRQHVMRKLGVRSLAALILCAVQTGEWIDS"
     gene            complement(3803..5509)
                     /gene="ybaL"
                     /locus_tag="VFV99_19275"
     CDS             complement(3803..5509)
                     /gene="ybaL"
                     /locus_tag="VFV99_19275"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEX5061522.1"
                     /translation="MAHETTLISVIAGGIGLAFVFGFVAARLRLPPLVGYLLAGIVLG
                     PFTPGYVVDTGLAGQLAEVGVILLMFGVGLHFSLADLLSVKRIAVPGAIGQIALATVL
                     GALVGRMFGWSWGSGIVFGLCLSVASTVVLLRALEDRGALESTDGRIAVGWLIVEDLV
                     TVVVLVLLPALAIPLGGEHRGPADRSLFAVIALTLGKVVAFVAVMLVIGRRAVPWLLE
                     RVARLGSRELFTLAVLAVALGIAVGAAALFDVSVALGAFFAGMVVNGSRLSHEAAKNA
                     LPLQDSFSVLFFVAVGMLFDPAILIDHPGKILWVLFIVMIGKSVGAFLIVRTFRYPTR
                     TAFTISASLAEIGEFSFILATLAVSLKLLPPAGQSLVVAGSIFSIALNPFAFWFAEHA
                     ARLFEKPAKSPVKVGAEHHGHAVIVGYGRVGSAIGETFITSHVPYVVIDADEDRVDAL
                     RNEGVPVVLGDGARPEVLHQAGITHARLLVVAVPDPYQAAAIVANAHTLSPLLATVAR
                     AHGDEERAYLEERGVEIAVVGERELALGMAHHALVHMGFADSEVGDVIDSFRHAHQPA
                     VH"
     gene            5702..7432
                     /locus_tag="VFV99_19280"
     CDS             5702..7432
                     /locus_tag="VFV99_19280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061523.1"
                     /translation="MGGAPEEPATVESAPPLDPTDSPTTAEKPSAMADASKRPPMPPS
                     EVSVDKGSNTSGTSVGSPIEALERDEILRTRTFSVLVIVFSVTGAGVALIVPGGDPRA
                     RSVMLGGCVVALASMLFMLYQTYDPVRFRKPLTSLVWFVPSIVVMSAVFFFGVFSPAP
                     MLVVLGIYFVGLSKAGRMAIASYVTCAALYTGMSLFILFGGSDFGVLHPTVTPEQHLL
                     VLFCVQLVLALTIVIARLSRRTALLAVGELEQATRLAAHRQALLLEAREELDRALRTK
                     RGRFSDQVIGNYQLGDVLGRGAMGEVYAATDIRTRATVAIKLLSQASLGNAHVVMRFF
                     RELRTAATVHSPHVVRVIEVGENPVPYLVMERLEGETLSELLRRRRMLPPAEVLELID
                     QIAIGVGAAAAAGIVHRDLKPQNVFKHRDAWKVLDFGVARAIDQGDTLTGGQVVGTPA
                     YMAPEQASGGTVDHRTDLYALAAIAYRALTGHPPFSAGDIAQTLYRVVHTRPTRPTLL
                     APELPVDIDVVLAIGMALQPADRFASGEDFASALRAAVDGTLPGDLRERAAAFPNGGW
                     GSSLRLRRAQ"
     gene            7650..8030
                     /locus_tag="VFV99_19285"
     CDS             7650..8030
                     /locus_tag="VFV99_19285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061524.1"
                     /translation="MSEAVLFVDDDEDLCEMMRAILTKLGVSRVVVARSLRDVEAQRD
                     DAITCTIAILDINLGNDQPSGVKVYEWLHREGFTGRTIFLTGHAGNDPRVQEAASLSG
                     SVIASKPLSVPELRDLIGGARQAI"
     gene            8027..8989
                     /locus_tag="VFV99_19290"
     CDS             8027..8989
                     /locus_tag="VFV99_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061525.1"
                     /translation="MTSVLWLAFGLSLGAAVLHAALGLTRPVDRTYLSFAFMMALLGP
                     YLYYEWVLYRATTGAEAVVAFRSQITIAHCFFGGLLVFVPAYTKARVPRWQMAALWGG
                     LALLFAINIIAPYGIWFSAEPELERLTFRGEPYTAVLAPPTVLQGVHTVYVLGLMVFT
                     IRCAIDLIRRGERQRGLVLAIAISVSVAQHLVDVVRDAIGGSWPYFAEFAIVSWGLIM
                     SIQLAIDFRTTRERLQETLSETEERAAQLAELVNASLHVRDKLNTPLQTLEIGLAMCK
                     PRNRDDIETLAILRRDVMELATLGRNVERAAQEQCFIPTEELAK"
     gene            8986..9933
                     /locus_tag="VFV99_19295"
     CDS             8986..9933
                     /locus_tag="VFV99_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061526.1"
                     /translation="MKLVAWAAIGITFGAAVLHAVLGLRRPRDRADLCFASMMAFLAL
                     YFYLGIAVDRTQSVDAAVEGVRYQVFALLGCHGSLLLFVPAYTNTRLPSSLTAAYWVG
                     LVILFAANLWSTYGLWFSGPPELVPSQFRHAHYQTVLAPPMSLLQHAYALYSTSYLCV
                     AAGCAASLFKHGGRQRAITFGCALLIVVVTNVVDLARHPVNGSLPYFAEFGFVAWALI
                     MSVQLARDFRAEAKALERALADVAAQSERLQCILDSLRALEVRMLAPIDELENGVATL
                     AATTQPETASLTRLRRAVARLRTVAHAMPDISTTRLAKA"
     gene            10070..11425
                     /gene="nhaA"
                     /locus_tag="VFV99_19300"
     CDS             10070..11425
                     /gene="nhaA"
                     /locus_tag="VFV99_19300"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5061527.1"
                     /translation="MSHTDHTSPPPETWAPARRAAQAITSPIQSVLAIEAASGIALLA
                     ATVFSLLWANVSPETYASVWHTPIGARVGSWSFERPFHFWVNDGLMTIFFFVVGLEIR
                     REIFEGELATLRKAALPLAAALGGMLAPAVIFVLLNIGRVGAPGWAIPMATDIAFAVG
                     VLTLLGSRVVHGQRVLLLALAVIDDIGSIVVIALFYNSSASLTGFPLIVGGLATTFVM
                     RSAGVRYPLLYLVPGALLWAGLLATGIHPTIAGVALGLLTPVRPWFGPSGFRETTQAH
                     LEELPVDDREALLTRLDAINQARREAVSPVERLIHALHPWVAFGVMPLFALANAGVPL
                     GGARFTGDYLWLFAGIVIGLVIGKPVGIASAALGANRLGVATRSADVTRRGIVLVGLV
                     GGIGFTMSLFIAQLAFEPGPLLDTAKLAILAGSATAIIVGLGFGVLAARPRKSAPPAS
                     V"
     gene            complement(11626..12393)
                     /locus_tag="VFV99_19305"
     CDS             complement(11626..12393)
                     /locus_tag="VFV99_19305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061528.1"
                     /translation="MESNTAELIAAARAGDRRAIDELLARYEPSIYRFGLRMCGDEES
                     AREVLQETMLAAFRGLSGFRGDASLSTWLFQIARSFCIKERRRPHVPHALDADVADPA
                     APPDAQLHARQIGLALAGAIAALPVEQREVILLRDVEGLSAQEAAAIVGIEVGALKSR
                     LHRARMALRERLVGVIESSDEPCAELTNQLSAYAAAEIDQAACTQIEAHLASCSRCVG
                     ACDALKRTVSLCKSLPGGAVPEPVRAAVRTAILDMIR"
     gene            12447..13184
                     /locus_tag="VFV99_19310"
     CDS             12447..13184
                     /locus_tag="VFV99_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5061529.1"
                     /translation="MVVLGLLLSLAIGLSLGLLGGGGSLLTLPVLHYVFNVSAHEAVA
                     MSLVVVAVTSLVALAMHARAGSVRWATGITFGAASMAAAFGGARIGSTLPDSIVLAAF
                     AITMLIAGTAMVLRRAPEPLGTTGSSITLRATMIGLGVGLLTGVLGAGGGFIVVPTLM
                     IGCGLALREAVATSVLVIAMNSLAALAAHATETTLDMPLVATVTSVAAVGSLLGIVLG
                     YRLSTKHLQRGFGWFVITIGLVMLVRG"
     gene            complement(13199..15409)
                     /locus_tag="VFV99_19315"
     CDS             complement(13199..15409)
                     /locus_tag="VFV99_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061530.1"
                     /translation="MDGAGDAKDDSFGGAGAKEDGQYSACQLAEVLKLVNESTSTADK
                     LESVGVADNAARAIVKHRVGPDGDAGTGDDDIFDDLNELDGVDYVGNLALGKLVYAVL
                     PRCENDLTTRPFIDSTTFTGQSGGWARDNEEVEVVLGVKGMTGQKLRELLLQKNSTGR
                     TLYERMRKARVMEAFTYGYALDEMPWTSDAQAARELLPYVSLTIEPGRYEPNDEGELE
                     ISLGTDLMDDTYYDTHGYSLLGNGIELRGRARWDDPTTVRRILIAAKFGTEVDADGNK
                     VNSKVDIRNDNGTSFIPKLDDDVRRGKVAWSGSDAPATPIRGVYEQLKMKNVLLDIGS
                     HTGVLLLEPAVHLRSTRSRYHMNEANLTNVRGVFQNATTRINAALAVVDRAQAAGIIP
                     AANRAAVDAMEAMGRGILDKSLIAQRLGTTVTALRLPDNWAQPGNATDLEANKKTAEA
                     INALYHEFATALDDTDRAITNAADESFDSFTDMFRLWRISQESTLARKTAWDPFLNSY
                     RSLSTATNRANAIAQFNTYGAAQKAANNDAFDDFVALDDAGWTRLGNYLEKMTLTTGE
                     RQIETAGIAARQLWFDQARTLYVPGSSRAFSNFMIDTTDMTEMLTHEEWLSIPEAERS
                     FAQPLPAAKVFNTVFVNELQIELGMEKAYVDRLRALEAQLAMNPNDAATKAQLEGARF
                     VWQQYTESMKVLTELKGDNVIDRLKRAGAPQGITWSAPPDSKGNIALKILADRD"
     gene            15617..16369
                     /locus_tag="VFV99_19320"
     CDS             15617..16369
                     /locus_tag="VFV99_19320"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HEX5061531.1"
                     /translation="MRRAAFLVATLSACASAESMPIDPFLDSSKGDGHPARLEELDHS
                     KLDMDEPSDLVQVDGKLYSVSDRHSKIYRVKPNGDADVHMNVDGTDLEAIGFDPDRGE
                     FLIADETKSKIWTIDAAGDRHDAIELDNADDGNSGIEGIVVAGNGHLYAVKEKDPARI
                     YELDADGTLLASKKIELADDLSAITYNADDDHLYVLSDEDRSLFRLDKHWNADRAWKL
                     PVDHPEGVAFDGTTLYVVSDGDHRIYTFELVD"
     gene            complement(16379..17263)
                     /locus_tag="VFV99_19325"
     CDS             complement(16379..17263)
                     /locus_tag="VFV99_19325"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5061532.1"
                     /translation="MQRSKIVCPVIAMLAVACASTTPSSAPDAAPSSVDATSPAFDAA
                     VASRCTETANELSCPYETASFQALVVMRSVRYQTPLGTPPPNGWPVVFFFQGSFHSAE
                     EAFHSTRDATFGQFQLVRTIRALLDGGYAVIAPEPLVDIAWQTNVPPYSLNWTATSDH
                     AFLLAIIAGIGNGTLGPLDTTRLYATGISSGGYMTSRMAVSYPGVFRALAVHSASYAT
                     CGVTCLLPLNLPVDHPPTLFLHGAQDPIVPVTQMLAYRDELAQQGHVVDTVIDAAAGH
                     EWIAPAVPAITAWFDAHP"
     gene            complement(17306..18940)
                     /locus_tag="VFV99_19330"
     CDS             complement(17306..18940)
                     /locus_tag="VFV99_19330"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5061533.1"
                     /translation="MSERSFVLGTMQDTPLTLSAIFQHGRAVYGDSEVVTYEGDGEFR
                     RATFAEVAKRAEQLACALARLGIERGDRVATFAWNTQEHLEAYLAVPAMGAVLHTLNI
                     RLFPEQVAYIIDHAEDAIILVDDSLVPVLAPLVPQLRTVKHIIVIGRGDASAIPSALR
                     YDELLVAERGGFAWPTLDERAAAAMCYTSGTTGHPKGVVYSHRSTYLHSLAACTTNSL
                     GIGERDRILPIVPMFHANAWGLPYAGWFAGSDLVMPNRFMQPDALAQIIASERPTFAA
                     AVPTIWSSLLACADRVDMDLSSLERIVCGGAAVPRSLMEAFDRRFGRRIIQAWGMTET
                     SPIAALAIPPTGTPPEREMTYRMRTGRILSGVEARIVDDNGCELPADGTQVGEIEVRG
                     PWITASYYRDPAPEKFDDGWLRTGDIGSLDPEGYLQITDRAKDVIKSGGEWISSVELE
                     NALAAHPDVVECAVIAVPDSRWSERPLACVVRKPGSTVLAEALRDFLAERVARWWLPE
                     RWSFVAEVPKTSVGKFDKKVLRARYAEGALVVAELS"
     gene            19048..19689
                     /locus_tag="VFV99_19335"
     CDS             19048..19689
                     /locus_tag="VFV99_19335"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5061534.1"
                     /translation="MTQLAHVSAARVPAAEAKLPQLGKTGTYRRILEEALMLFAQTGY
                     HGTSVRDIASAVGIQIAALYVHFPSKGHVLAELCRIGHEEHQRALRTALLDAGSSPAD
                     QLAALVRAHVRLHAEYAMLATVANYELHALPDELAGATMALRRQSEALLIEVCERGEA
                     QKKFTTPDVVITAAAIGAMGMRVAQWYRADLGKTIDEIANVHAELALRMFGAR"
     gene            19710..20516
                     /locus_tag="VFV99_19340"
     CDS             19710..20516
                     /locus_tag="VFV99_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5061535.1"
                     /translation="MPIAELPNLCMSYQEAGSGEPIVLLHGLGSCAHDWEEQIEAFAS
                     SYRVIAPDLRGHGDTDKLTRSTIAAMAEDVVQLLAELGIQSVHVVGMSMGGMIAFQLA
                     LDAPQLVKSLVIVNSGPDAVPRTLRHRFAIFTRFALLRVVGLPRLTRVIANKLFPASG
                     QEMLRRRFVERASTNDPTAYKAALDAIVSWSVADRLSSVVAPTLVISADQDYTPVSFK
                     ADYVAKLPNARLVVIPDSRHATPMDQPARFNATVLEFLAGREHRINAVAG"
     gene            20752..23277
                     /locus_tag="VFV99_19345"
     CDS             20752..23277
                     /locus_tag="VFV99_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5061536.1"
                     /translation="MPLWAQPQPSPQATGQPSPGQTAPAPPADQPSTPGADPSADSPP
                     PTAPPADSPAASPPASSPTNNELARIRGVIRSGDHNEPVPGATIMLSGTTNAAISESD
                     GSYLIEGVVPASYTLEISAPGYADTTIAAKVEPAKTAVVDVNIGEMNAGEVIVVTGSR
                     SPEKLLDSPATIEYVSTNDIRRATGASYLSALSKVKGVDYADAGLGDQRISMRGFTTQ
                     FNSRLLQMIDGRIAQMPGTGLPQGNSLPTTSLDMKSIEVIVGPASALYGPNAHTGVIN
                     VITKTPWDESGATVAVRGGSQNLIDLGARIAGTVKDDFGWKLNGQFMRAEDFAPDVMR
                     PEHYYNTPFFERDLVGGSYDVKGGKLDGTLYYKLPDDWIVKSTYGWSYGDGFAITNNG
                     RNHLRGVQTQVQNIQLSGKHLYAQVTRTASSIGNAYPIDGLARAVAAMGATTLPSPDV
                     LEQMRTALKLVDDSQLYDAEAQYRATVAGVEATTGVQGRVYLPDSGGTYLADANGENI
                     NATEIGGYGQASYKLLDDRLRLVGALRVDHHSDYGTQLSPKAAVVVKVAENHNLRAGY
                     NHALKSPTILESNLLIQNMFRGNKTGYVIKDDTGTIVDTLAPLSPERVNSFEVGYKGI
                     VDDRLFIDAVAYDSYYRNFISPLTATANPAMNTFAYFPDGTLVGDGTPTAGALYTYRN
                     FGAAHVRGTDVGVSWRPVDTLDLSSSVSAIDLVDFTQSNPSVAPLLLNVPALKLKGSA
                     TISNLGMANYFVNLSGRWQSAYKFESGYWSSSRFYADNDGKLPARFVADIAAGYTFPK
                     QNLKLTATVANVFDDHNVDLLGSPIPGRLAFVQVEYAYQGLTF"
     gene            23303..24094
                     /locus_tag="VFV99_19350"
     CDS             23303..24094
                     /locus_tag="VFV99_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5061537.1"
                     /translation="MPTTSLERSGGIATLWLDRADKRNAMDADLMKGLAAAFDEIGGD
                     RSVRVVVLRGRGNVFSSGIDHTLLTQVFGATQSSPFLHLHHQLQDTFHRMSRLHQPVI
                     AAMHGVCVGMALELALAADIRIASEDCVMGLPEIAFGIVPDVGGTTRLVRAVGEPRAR
                     ELILTGRLVKAATAERYGLVHEITGNGDVLAQRAMVVAEQLSAHSGTALGLAKTLCSA
                     SADADAATSFRLEGVVQQTLLAQPDLMTRFPAALAFIKAQLANPG"
     gene            24135..>25113
                     /locus_tag="VFV99_19355"
     CDS             24135..>25113
                     /locus_tag="VFV99_19355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX5061538.1"
                     /translation="MALEVLRDVFGYDAFRGGQREAVEAVLGGRDALVLLPTGAGKSL
                     CYQVPAIVRARRGEGTTIVISPLIALMNDQVRALTAKGVAAAALHSQCDDAERLETIA
                     RLTRGELALLYISPERAVLDGFKRLLGRAHIAMVAIDEAHCVSQWGHDFRPEYMRLAE
                     LRAVASSTMATAPMIALTATATPRVMDEIATALELRMPTIARGDFRRPNLAFEVIAAG
                     GDDARIAATIDALERAGMRGRSGAGRAIVYCSTRKKAEDVAAKLKQAGFAAGHYHAGR
                     TALARERAQSGFSLGRMRVLVATNAFGMGIDYPDVRVIVHFQAPGSLEAY"
BASE COUNT         4298 a         8394 c         8031 g         4390 t
ORIGIN      
        1 acgaaggaga cgctgcatcg ctacacgcag atcgtcggca agatccaact cgggcgaacg
       61 ccgatcgaga atcacttctg gaacgtcgcg ctgcgcatga cttcgcgcgg tctcgcaacg
      121 tccgcactcg agtacgaggg acgcacgttc gatatcgaga tcgatctcgt cgagcacgcc
      181 gtccacatcc gcacgagtga cggcggccgc gcgaaagtcg agctgcgtcc acttgccgtc
      241 gcggatttct atcgcgagat ccaggaggcc ctggaccgac tcggcatcga tgttccgatc
      301 tgggatcacc cggtcgagct caagaccgac gtgattccgt tctcacaaga ccgcatgcac
      361 gccgagtacg accgcgagta cgcgaatcgc ttcttccacg tgctgtccag cgccaccgag
      421 gttctcgaag agttccgttc gcgattcacc ggcaagtgca gtggcgtcgg cttctactgg
      481 ggcacgttcg atgtctcggt cgcgatgtat tcgggtcgcg ccgtcgagaa cgcgccgacc
      541 gaatcgcgga tcgagcgcga ggcctactcg cacgagctga tcgaggtcgg gttctggccg
      601 ggcgatgccg tgtatcagca gccggcgttc tttgcgatgc aatggccgat gcccgagtcg
      661 tatgcgcacg cacacctcga gcccgcagat gcgagctggc tcgagccaag ccgctgcttc
      721 gtgctgccat acgagagcgg tcgcaacggc gactacacgg cgcgcgtgct cgacttctgc
      781 caatcggcgt acgagaccgg cgcgaacatg gccggttggg accctgggct agagcgcaac
      841 catgaaatgt gggccgacca ctagaagcaa ccgcgcacgc cgtcgacgtt caggcgacac
      901 accgcacgga ctgcattgcg gaagctgatc cacagccggt gatcggtgcc gtcgatcgat
      961 gcgacgagct cgttgcgaaa cgttcgactc atctcgagct gaacgccgag atgtccgccg
     1021 gtgcgattgg agatgttcgc gcttgcggtg ccggtgatgc ccgcgcagtc gctgtcgccg
     1081 ggtgcgtcga ccgcgatcgc accgctcgcc gagtactgcg cggtgtacgt gtcgtagtag
     1141 tgcttgaacg ccgtgcgcag cgcgggcgtg atgccgccga cgcagatctt gttcgggcca
     1201 ccgtggccgt ggatcgcgat gccgcgattc acgctccgca tgagtgactc ggcacgtgaa
     1261 tcgttgaagt gggtcgacgt gacgtgcagt gcacgagccg agcgcgtcgg cccgccgtac
     1321 ttgtcgaggc aggtgtcggt tggatgcgcg acgaggacgt atgacggcag atgcagatcg
     1381 gccgcgagct ggcgcgcgat gcgatcggtc cccagctcga tgccaccacc atgcggcgcg
     1441 acaatgccca tggtcatttc ggctgcggtc ggcgcgacga tcacgtcgta gctggcacct
     1501 tcgcggcaca gcgtgctgag gctcaagttc ggcgaaccct ggcacgagta accaccattc
     1561 accgacgaag cggactcggg acgaaagcac gcgaagctat ccggcgtcgt cgcgccgatc
     1621 gtcgtgacct ccgattgctc gtcgggaaca tcaactgagc cgacgtcggc gatgcagcca
     1681 gtgaatgcga agactgcgaa cggtgcgagg cgattcatgc ggtggccgcg tgcaagtaga
     1741 agaccgccgc taccagctcg caatcccgtg cggccacgat ctgcgcctga gggccgaacc
     1801 cctcacatca gcgacgatcc gcaccgctgc cgcagaccgc gacaaccatg tcgggctctc
     1861 gggaaggcag cccccaagcg ccggaccgaa cctcgcgaga tcacgctggc gggcctggca
     1921 ccgcgcttgg acactattgc gacatgggtt cacgttggtt gcagcgcgtc acgcttgcag
     1981 ttgccgccgg cgcgtcgatc gcgacgacgc agctgcccca ggaatggcat gccgatgcat
     2041 cggcggtcct cgatgccgtc gtgtttgaca cccagcgcgt gacggcgagc tacgcgatcc
     2101 acgccgagat cgctggcacc ggcaagtaca ccgatctgga cggcacactc accgcaacga
     2161 tcagcgcgac cacggccggc acgttcgatg cgcaatcgca gcttcggctc acgttgcgct
     2221 cgacgacaaa cccggacgtc cagtccgtct cggaggtcac gctgccaaac atcgatccgc
     2281 gcgtagcggc cgagcttccc gcatggaccc actgcatgac gccgccatgc gccgaagact
     2341 tcgagctcac cgtcgagctc atcggcaccg gtgcaccgcc actgaacatc accggcaacg
     2401 tcggcgtgcg ctcgctcggc accgacaaca agctcgaagc gacaacgcag accacgatca
     2461 tcgcgagccc actgccgtga tcgcgacgcg cacgctcatg catccgatct cgctcggcgc
     2521 gatcgcgttg ctcgtgatca atgatcacgt gctcaagcat gtcgcgcctg gcgtgatcac
     2581 cggcaagctc tccgacttcg caggtctcgt cttcttcccg ttgctacttg ccgctgccgc
     2641 ggagcacgca ggaatccggc gcggcatcca gaccgtcatc gtggcagcga tcgcgacggc
     2701 tgcggcgttc gcggcgatca agctttccgc gtctgcggcg gagatctacc gcaacgggct
     2761 cggagtcctg cagtggccgt ttcgagcagc gcgcgccatg ttgctcggcg aatccgtacc
     2821 gagcgtcggc cgtgtcgcgc acgtggtcga cgccactgac ctggtcgcgc ttgtcgcact
     2881 cggcgttccg atcgcgctcg ttcatcgcgc catgcgattc ccgtccactg tacccgcgaa
     2941 atgcgccggt tctaccgaaa gcgtttaccc gcagtgactg gagtgtgtct ccacgtcgca
     3001 gttggatgcc cgcggacgcc aatttgcgcg cgatcgcaca cggacggccc cgcgtcctgc
     3061 gccgacaccg ggccccaggt tgtttgtttc agaacgggct gattaactgt ttaacagtga
     3121 aggatgtgag agaagagggc acggatgtgg cgaggattcg cggagtgctc ctcgtcgacg
     3181 gccatcccat cgttcgcgac ggcctcgcgg catggatcga cagccaagcc ggtctcgaag
     3241 tcgtaggtca cgcggcgagc gccgacgagg cacaagcgat caagaggcaa ctgaatcctg
     3301 acctcgtcgt tgtcggggtc gcactccgca acagcacggg catcgagctc acgcgccgct
     3361 tggtcgcacg acgtgacgta tgcattctcg gactctctgg gctgcccggt cccggtctcg
     3421 cgatgcaagg cgcgcgaatg ctgctggctg gcgcgacagg ctttgctctc aagtcacagc
     3481 cgtcgaagga aatcctcgac gcgctgcgga tgacgctcga acgcaaatgc tacatcacgc
     3541 ccgcgatcga ggccgaagtc aaaacactcc tgcacggacc atcgacagtc gccgcgagtc
     3601 agctgaccgt ccgcgagcgt gagatctgga agctcgtggt caaaggctac agcaccaaag
     3661 agatcggaca ggcgctgttc atctcgccga gaaccgtcga ggtgcatcgt cagcacgtga
     3721 tgcgcaagct tggtgttcgt tcgctcgcgg cgctgatcct gtgtgccgtg caaactggcg
     3781 agtggatcga ttcgtagcca ggtcagtgca ccgcaggttg gtgcgcgtga cggaacgaat
     3841 cgatgacatc gccgacctcg ctgtcggcga aacccatgtg gacgagcgcg tgatgcgcca
     3901 tgccgagcgc gagctcgcgt tcgccgacga ccgcgatctc gacgccgcgc tcttcgaggt
     3961 aggcacgctc ttcgtctccg tgcgcgcgcg caaccgtcgc gagcaacggg ctcagcgtgt
     4021 gcgcgttcgc gacgatcgcg gcggcttgat atgggtcggg tactgcgacc acgagcaggc
     4081 gcgcgtgcgt gatcccagcc tggtgcagca cctcggggcg cgcgccatcg ccgagcacga
     4141 ccggaacacc ctcgttgcgt agcgcgtcca cgcgatcctc gtcggcgtcg atcacgacgt
     4201 acggaacgtg gctggtgatg aacgtctcgc cgatggcgct gccgacgcgg ccgtacccga
     4261 cgatcacggc gtggccatgg tgctcggcac caaccttcac gggagacttc gcaggcttct
     4321 cgaacaaccg cgccgcgtgc tccgcgaacc agaacgcgaa gggattgagc gcgatcgaga
     4381 agatcgatcc cgcgacgaca aggctttggc cagcgggcgg caacagcttg agactcacgg
     4441 cgagcgttgc gagaatgaac gagaactcgc cgatctccgc gaggctcgca gagatcgtga
     4501 acgccgtgcg cgtcgggtag cggaacgtac ggacgatcag aaacgcgcca accgacttgc
     4561 cgatcatcac gatgaacagc acccacagga tcttgcccgg atggtcgatc aagatcgccg
     4621 gatcgaacag catgccgacg gcgacgaaga acaacaccga gaacgagtcc tgaagcggca
     4681 gcgcgttctt ggcagcctca tggctgagtc gcgatccatt gacgaccatg ccggcgaaga
     4741 acgctccgag cgcgaccgac acatcgaaga gcgctgcggc gccgaccgcg atcccgagtg
     4801 ccaccgccag caccgcaagc gtgaacagct cgcgcgaacc gagtcgcgcg acgcgctcga
     4861 gcaaccacgg caccgcgcgc ctgccgatca cgagcatcac ggccacgaac gcgaccacct
     4921 tgccgagggt caacgcgatc acggcgaaca agctgcgatc ggcggggccg cgatgctcgc
     4981 cgccgagcgg gatcgcaagc gcaggcagca acacgagcac gacaacggtc acgaggtctt
     5041 cgacgatcaa ccagccgacc gcgatccgtc catcggtgga ctcgagcgcg ccgcgatcct
     5101 cgagcgcgcg gagcaacacg acggtgctcg cgaccgatag acacagaccg aacacgatgc
     5161 ccgatcccca cgaccagcca aacatccggc cgacgagcgc accgagtacg gtggcaagcg
     5221 caatctgccc gatcgcaccg gggaccgcga tccgcttcac cgacaacaga tcagccagcg
     5281 agaaatgcag accgacgccg aacatcagaa gaatgacgcc gacctcggcg agctgacccg
     5341 caagcccggt gtccacgaca tatccgggcg tgaacggccc gagcacgatg ccggcgagga
     5401 gatatccgac gaggggcggc aaccggagac gcgcagcgac gaagccgaag acaaaggcga
     5461 ggccgatgcc gccggcgatc acactgatga gagtggtctc gtgagccatg acacgcgccg
     5521 tgtcgacgag aattgcgccg acgatcgttg gtaccagcat ttcgcgacgt cgccacgagt
     5581 gcgatcacgc ccgccgtgcg ctccgaccac acgaacggtt tgcggctgta cgcattctcc
     5641 tcacagcggg ccaatcgcgg ctccgctcga cactgccacg agcgagcgag atgatcatgt
     5701 aatgggtggc gcgccggagg agccagcgac ggtcgagagc gcgcccccgc tcgatccgac
     5761 agattccccg acgacagcgg agaaaccgtc cgcgatggcc gacgcgagca agcggccgcc
     5821 catgccgccg agcgaggtga gcgtcgacaa gggatcgaac acgagtggta cgagtgtcgg
     5881 atcgccgatc gaagcgctcg agcgcgacga gatcttacgc acgcggacgt tcagcgtgct
     5941 cgtcatcgtg ttctcggtca ccggcgcggg tgtcgcgttg atcgttcccg gcggcgaccc
     6001 gcgcgcacgc agcgtcatgc tcggcggctg cgtcgtcgcg ctggcgtcga tgctgttcat
     6061 gctgtaccag acctacgacc cggtgcggtt tcgaaagccg ctcacgtcgc tcgtctggtt
     6121 cgtcccgtcg attgtcgtga tgtcggcggt gttcttcttc ggggtgtttt cgcccgcgcc
     6181 gatgttggtc gtgctcggca tctacttcgt cgggctcagc aaggcgggcc gaatggcgat
     6241 cgcgtcgtac gtcacgtgcg cggcgctcta cacgggtatg agcttgttca tcctgttcgg
     6301 cggcagcgac ttcggcgtgt tgcatccgac cgtcacgccg gagcagcact tgctcgtcct
     6361 gttctgcgtg cagctcgtac tcgcattgac gatcgtgatc gcgcggctct cgcgacgcac
     6421 tgccctgctc gcggtcggtg agctcgaaca ggctacgcgg ctcgcggcgc atcgccaggc
     6481 gctgctcctc gaagcgagag aggagctcga tcgcgcgctg cgcaccaagc gcggtcggtt
     6541 ctccgaccag gtgatcggca actaccagct cggcgacgtg ctcggtcgcg gcgcgatggg
     6601 cgaggtctat gcggccaccg atatacgaac gagagcgacc gtcgcgatca agctgctgtc
     6661 acaagcgtcg ctcggcaatg cgcacgtcgt gatgcggttc tttcgcgagt tgcgcacggc
     6721 cgctaccgtg cattcgcccc acgtcgtgcg cgtcatcgag gtcggcgaga atccggtgcc
     6781 gtatctcgtg atggaacggc tcgagggcga gacgctatcg gagctgctgc gccgccgccg
     6841 catgctgccg cccgccgagg tcctcgagct gatcgatcag atcgcgatcg gtgtcggcgc
     6901 tgctgccgct gccggcatcg tgcatcgcga tctcaaaccg cagaacgtgt tcaagcatcg
     6961 cgatgcgtgg aaggtgctcg acttcggcgt cgcgcgcgcg atcgatcagg gcgacacact
     7021 caccggcggg caagtcgtcg gcacgccggc gtacatggcg cccgagcagg ccagcggagg
     7081 caccgtcgat caccgcaccg atctgtacgc gctcgcagcg atcgcgtatc gcgcgctcac
     7141 cggccatccg ccgttttccg ccggcgacat cgcgcaaacg ctctatcgcg tcgtgcacac
     7201 ccgaccgact cgcccgacgc tgctcgcacc cgagctgcct gtcgacatcg acgtcgtcct
     7261 cgcgatcggg atggcgctcc aaccggcgga tcgtttcgca tcgggcgagg acttcgcgag
     7321 cgcactgcgc gcggccgtcg acgggacgct gccaggcgat ctgcgcgaac gcgcagccgc
     7381 gttccccaac ggaggttggg gtagctcact acggttacgt cgcgcccagt agacttcgtc
     7441 gatgcgatct cgtgtccgaa ttattttggc gagatcgtcc agtggtgcgg cgaatcttgc
     7501 gccgcgcgta ctcgcacacc acgcgtagta gcgctcgctg tttcctgact acccgcgagc
     7561 gcacaaggcg ctcatcccgt ttgtcgtgta gagcgcccgc ggtacggcgc gagcatcggc
     7621 agcgctattg atcggacgta gacttctaca tgtcggaagc ggtgctgttc gtcgacgacg
     7681 acgaggatct ctgcgagatg atgcgcgcaa tcctgaccaa gctcggggta tcgcgcgtcg
     7741 tcgtcgcgcg ctcgctacgg gatgtcgaag ctcagcgcga cgatgcgatc acgtgcacga
     7801 tcgcgatcct ggatatcaac ctcggcaacg atcagccaag cggcgtcaaa gtttacgagt
     7861 ggctccaccg cgaagggttt acaggccgca cgatctttct gaccggccac gctgggaatg
     7921 acccacgcgt ccaggaggct gcgagcttga gtggctcggt gatcgcttcg aaaccactct
     7981 ccgtcccaga gttgcgggac ctgatcgggg gcgcgagaca agcgatatga cgagcgtgct
     8041 gtggctcgcg ttcggcctct cgctcggcgc ggccgttctg cacgcagcac tcggcctgac
     8101 tcgaccggtc gatcgaacgt acctgtcgtt cgcattcatg atggcgctgc tcgggccgta
     8161 cctctactac gagtgggtgc tgtatcgcgc gacgacaggc gccgaggccg tcgtagcgtt
     8221 ccgctctcag atcacgatcg cacactgctt tttcggcggc ttgttggtat tcgtccccgc
     8281 gtatacgaag gctcgcgtgc cgcgctggca gatggcggca ctttggggcg gactggcgct
     8341 gctcttcgcc atcaacatca tcgctcccta cggcatctgg ttctcggcgg agcccgagct
     8401 ggagcgcctg acgttccgcg gcgaaccgta caccgcagtc ctcgcaccgc cgaccgtgct
     8461 gcaaggcgtc cacaccgtct acgtcttggg cctcatggtg ttcacgatcc gctgcgcgat
     8521 cgatctgatc cgacgcggcg aacgacagcg cggcctcgtg ctcgcgatcg cgatctcggt
     8581 gagcgtggcg cagcatctcg tcgatgtcgt ccgcgatgcg atcggtggct cgtggccgta
     8641 cttcgccgag ttcgcgatcg tgagctgggg actgatcatg tcgatccagc tcgcgatcga
     8701 tttccgcaca actcgtgagc ggctgcagga aacgttgagt gagaccgaag agcgcgccgc
     8761 gcagctcgcg gagctcgtca acgcctcgct gcacgtgcgc gacaagctca acacgccgct
     8821 ccagacgctc gagatcggac ttgcgatgtg caagccacgc aaccgcgacg acatcgagac
     8881 gctcgcgatc cttcggcgtg acgtcatgga gctcgcaacg cttggacgca acgtcgaacg
     8941 ggcggcgcaa gagcaatgct tcatcccgac ggaggagctt gcaaagtgaa actggtggcg
     9001 tgggccgcga tcggcatcac attcggcgcg gcggtgttgc acgccgtgct tggcttgcgc
     9061 cgtcctcgcg atcgagccga tctgtgcttc gccagcatga tggcgtttct cgcgctctac
     9121 ttctatctcg ggatcgccgt tgaccgaacg cagagcgtcg acgcggccgt cgagggcgtg
     9181 cgctatcagg tgtttgctct cctcggttgc cacggctcgc tgctgctgtt cgttcctgcg
     9241 tatacgaaca cgcgactgcc ttcctcgctc accgcagcgt actgggtcgg gctggtcatt
     9301 ctgttcgcgg caaatctctg gtcaacgtac gggctctggt tctcgggccc gccggagctc
     9361 gtcccgtcac agtttcgcca cgcccactat cagaccgttc tcgcgccgcc gatgtcgcta
     9421 ctgcagcatg cctatgcgct ctattccacg agctacctgt gcgtggcagc aggatgcgcc
     9481 gccagcctgt tcaagcatgg tggacgacag cgtgccatca cgttcggctg tgccttgttg
     9541 atcgtggtcg tgacgaacgt cgtcgatctc gctcgtcacc cggtaaacgg atcgctgccg
     9601 tacttcgccg aattcggctt cgtcgcatgg gcgctgatca tgtcggtgca actcgcgcga
     9661 gacttcaggg ccgaagccaa agcgctcgag cgcgcgctcg cagacgtcgc ggcgcagtcc
     9721 gagcggctcc agtgcatcct cgattcgctg cgtgccctcg aagtcaggat gctcgcgccg
     9781 atcgacgagc tcgagaacgg agttgcgacg ctcgccgcca caacgcaacc cgagaccgca
     9841 agtctgacga gactccgtcg ggcggtcgcg cgcctgcgca cggtcgcgca tgcgatgccg
     9901 gacatcagta ccacccggct cgcaaaggcg tagcgcaccg taggcgactc cccttttgcg
     9961 ccgcgggtgt acatgctaac taatgactga caactgaacg ggtgcggcca tggttgccgc
    10021 agggcttcgg tgccgatggg gtcgtcggca caacctctcc gaggagtcga tgtcccatac
    10081 cgatcacaca agtcctccgc ccgagacctg ggcgcccgct cgacgcgcag cgcaggcgat
    10141 cacgtcgccg atccagagcg tgctcgcgat cgaggcggcc agtggcatcg cgctcctcgc
    10201 ggcgaccgtg ttctcgctcc tgtgggccaa cgtctcgccg gagacctacg catcggtctg
    10261 gcatacgccg atcggcgcac gcgtcggctc gtggtcgttc gaacgaccgt tccacttctg
    10321 ggtcaacgac gggctgatga ccatcttctt cttcgtcgtc ggcctcgaga tccggcgcga
    10381 gatcttcgaa ggcgagctcg ccaccctgcg caaagcagcg ttgccgctcg cagcagcgct
    10441 cggcggcatg ctcgcgcccg cggtgatctt cgtactcctc aacatcggcc gagtcggcgc
    10501 gcccggttgg gcaatcccga tggcgacgga catcgcgttc gcggtcggcg tgcttaccct
    10561 gctcggctcc cgggtcgtgc atggtcagcg cgtcctcctg ctggcgctcg cggtgatcga
    10621 cgacatcggc tcgatcgtcg tcatcgcgct gttctacaac tcgagcgctt cactcactgg
    10681 ttttccgctg atcgtcggcg gactcgcgac gacgttcgtc atgcgttcgg caggtgtgcg
    10741 ctatccactg ctctacctgg tacccggcgc gctcctgtgg gcaggcctgc tcgccaccgg
    10801 gattcatccg acgattgcgg gtgtcgcgct cggtctgctc acgcccgtgc ggccgtggtt
    10861 cggcccgtcg ggcttccgtg aaacgacaca agcccatctc gaagagctgc cggtcgacga
    10921 tcgcgaagcg ctgctcacgc gcctcgatgc gatcaatcaa gcgcgccgcg aagccgtgtc
    10981 gccggtcgag cgactgatcc acgcgctgca tccgtgggtc gcgttcggcg tgatgccgct
    11041 gttcgcgctc gcgaatgccg gcgttccgct cggcggtgcg cgcttcaccg gcgactatct
    11101 atggctgttc gccgggatcg tgatcggcct cgtcatcggc aagccggtcg gcatcgcgtc
    11161 tgccgcgctc ggcgcgaacc gccttggcgt tgcgacacgg tccgccgacg tcacacgccg
    11221 cggcatcgtc ctcgtcgggc tcgtcggtgg catcggtttc acgatgtcgc tgttcatcgc
    11281 ccagctcgcg ttcgagcccg gaccgctgct cgacaccgcg aagctcgcga ttctcgcagg
    11341 ctcggcgacc gccattatcg tggggctcgg gtttggcgta ctcgccgcgc gcccgaggaa
    11401 gtcagcgcca cccgctagcg tttgaacagc ttggaccaca cgctctcggt cttggccggc
    11461 gcgggttgct cgcggcagcg acagcgctcc gctaccggaa cgtcaccgag cacgctctcg
    11521 atatgccggc cacaccctgc atagctgggt ttcttgcacg ttccacattc gattcgtctg
    11581 cacatatcgg gtgagagcct cgggctcgcg aaaggattcg cacgttcagc ggatcatgtc
    11641 gaggatcgcg gtgcgaactg cagcccgcac cggctccggc accgcgccac cgggcaggct
    11701 cttgcacagc gaaaccgtac gtttgagcgc gtcgcaggcg cccacgcacc gcgagcacga
    11761 agccagatgc gcttcgatct gcgtgcacgc tgcctggtcg atttctgcag cggcatacgc
    11821 ggacagctga ttcgtcagct ccgcgcacgg ctcgtcgctg ctctcgatca cgccgacgag
    11881 gcgctcgcgc agcgccatcc gcgcacgatg cagtcggctc ttgagtgcgc ccacttcgat
    11941 gccgacgatc gccgctgcct cctgagcgct caagccctcg acgtctcgca acaggatgac
    12001 ctcgcgctgc tcgaccggga gcgcagcgat cgcgccggca agagcgagcc cgatctgacg
    12061 cgcgtgcaac tgggcatcgg gtggcgccgc aggatccgca acgtccgcgt cgagcgcgtg
    12121 cggcacgtgg ggacgccgtc gctccttgat gcagaagctg cgcgcgatct gaaacagcca
    12181 cgtcgagagt gacgcatcac cgcgaaatcc ggagagcccc cggaacgccg ccaacatcgt
    12241 ttcttggaga acctcgcgcg cgctctcttc gtcgccgcac atgcgcaagc caaaccgata
    12301 gatcgacggc tcgtagcggg caagtagctc gtcgatcgcg cgtcgatcgc cggctcgcgc
    12361 ggcggcgatc agctcggcgg tattgctctc cacaccgaat cgatagcacg cccgaatcct
    12421 tttcgcggca gctggctctg acggacgtgg tcgtcctcgg tctcctcctg tcgctcgcga
    12481 tcgggctttc cctcggcctg ctcggtggag gcggctcact cctcacgctg cccgtcttgc
    12541 actacgtgtt taacgtcagc gcgcacgagg cggtcgcgat gtcgctcgtc gtagtagcgg
    12601 tgacgagtct cgtcgcgctc gcgatgcacg cccgcgctgg ttccgtgcgg tgggctaccg
    12661 gcatcacgtt cggcgctgca tcgatggcag ccgcatttgg cggtgcgcgc atcggcagca
    12721 cgctgccaga ttcgatcgtg ctcgcggcct tcgcgatcac gatgctgatc gcgggcaccg
    12781 cgatggtgct ccgccgcgca cccgagccgc taggcacgac cggctcgagc atcacgctgc
    12841 gcgcgacgat gatcgggctg ggcgtcggcc tcctcaccgg cgtgctcggc gccggcggtg
    12901 gcttcatcgt cgtcccgacg ttgatgatcg gctgcggcct ggcgttgcgc gaagcggtgg
    12961 cgacgtccgt gctcgtgatc gcgatgaatt cgctcgctgc actcgccgct cacgccaccg
    13021 aaacgacgct cgacatgccg ctcgtcgcca cggtcacgag tgttgcggcg gtgggcagcc
    13081 tcctcggtat cgtgctcggg taccgactgt ccacgaaaca tctccagcgc gggttcggct
    13141 ggttcgtcat caccatcggt ttggtgatgc tcgtgcgtgg ctgagccgcg cgtacagtct
    13201 agtcgcggtc agcgaggatc ttgagcgcga tgttgccctt cgagtcgggc ggcgccgacc
    13261 acgtgatgcc ctgcggagca ccggcgcgct tcaggcgatc gatgacgttg tcgcccttga
    13321 gctcggtcag caccttcatc gactctgtgt actgctgcca cacgaagcgc gcgccttcga
    13381 gctgcgcctt ggtggctgcg tcgttcggat tcatcgcgag ctgcgcctcg agcgcgcgca
    13441 ggcgatcgac gtacgccttc tccatgccga gctcgatctg gagctcgttg acgaacacgg
    13501 tgttgaacac cttcgcggcg ggcagcggct gcgcgaacga gcgctcggcc tcggggatcg
    13561 agagccactc ctcgtgggtc agcatctcgg tcatgtcggt cgtgtcgatc atgaagttcg
    13621 agaacgcgcg gctcgagccc ggcacgtaga gcgtacgcgc ctgatcgaac cacagctgac
    13681 gcgcggcgat gccggcggtc tcgatttgcc gctcgccggt cgtgagtgtc atcttctcga
    13741 ggtagttgcc aaggcgcgtc cagcccgcgt cgtcgagcgc gacgaagtca tcgaacgcgt
    13801 cgttgttcgc agccttctgc gcggcaccgt acgtattgaa ctgcgcgatc gcgttcgcgc
    13861 ggttcgtggc agtcgacaag ctgcgatacg aattcaggaa cggatcccac gcggtcttgc
    13921 gcgcgagcgt cgactcctgg ctgattcgcc acaggcggaa catgtcggtg aagctgtcga
    13981 agctctcgtc cgccgcgttc gtgatcgcgc gatcggtgtc gtcgagcgca gtcgcgaact
    14041 cgtgatagag cgcattgatc gcctcggcgg tcttcttgtt ggcttcgaga tcggtcgcgt
    14101 tgcccggctg cgcccagttg tcggggaggc gcaacgccgt cacggtcgta ccgaggcgct
    14161 gtgcgatcag cgatttgtcg aggatgcccc ggcccatcgc ttccatcgcg tcgactgcag
    14221 cgcgattcgc ggccgggatg atgccggctg cctgcgcgcg atcgaccacc gcgagcgcgg
    14281 cgttgatgcg cgttgtcgca ttctgaaaca cgccgcgaac gttcgtgagg ttcgcttcgt
    14341 tcatgtgata gcggctgcgc gtcgagcgca gatgtaccgc gggctcgagg agcagcacgc
    14401 ccgtatgcga tccgatgtcg agcagcacgt tcttcatctt gagctgctcg tacacgccac
    14461 ggatcggcgt cgccggcgcg tcactgccgc tccacgcgac cttgccgcgg cgaacgtcat
    14521 cgtcgagctt cgggatgaag ctcgtgccgt tgtcgttgcg gatgtcgacc ttgctgttga
    14581 ccttgttgcc atcagcgtcg acctcggtgc cgaacttcgc agcgatcagg atgcggcgaa
    14641 ccgtcgtcgg gtcgtcccac cgtgcgcggc cgcgcagctc gatgccgttg ccgagcagcg
    14701 agtagccgtg cgtgtcgtaa tacgtgtcgt ccatgaggtc ggtgccgagc gagatctcga
    14761 gctcgccctc gtcgtttggc tcgtagcgac cgggctcgat cgtcagcgac acgtacggca
    14821 gcagctcgcg cgcggcctgc gcgtccgacg tccacggcat ctcgtcgagg gcgtagccat
    14881 acgtgaacgc ctccatcacg cgcgccttgc gcatgcgctc gtacagcgtg cggccggtgc
    14941 tgttcttctg cagcaacagc tcgcgcagct tctggcccgt catacctttg acgccgagca
    15001 cgacttcgac ttcctcgttg tcgcgtgccc agccgccgct ctggcccgtg aacgtcgtgc
    15061 tgtcgatgaa cggccgcgtc gtcagatcgt tttcgcagcg cggtagcacc gcgtacacga
    15121 gcttgccgag tgcgaggttg ccgacgtagt cgactccgtc gagctcgttc aagtcgtcga
    15181 agatgtcgtc gtcacccgtg cccgcatcac catccggtcc gacgcggtgc ttgacgatcg
    15241 cgcgcgcggc attgtcggcc acgcccaccg actcgagctt gtccgccgtc gacgtcgact
    15301 cgttgacgag cttgagcacc tcggcgagct ggcaggccga gtactggccg tcctctttcg
    15361 ctcctgcgcc accgaacgag tcgtccttgg cgtctcctgc accgtccatg caaccaactg
    15421 tgagcactag aattgcgagc ctgcgcatgc ccactcgctc tacaagcaac gtgcctctcg
    15481 caggtcacgt gacaaagccg gcttacgttc tttgcaggct cgtcgcgtag ccggttggct
    15541 ggcgtgtcgc gtagccgtcg ttcgtcacat ttcggcggct tgtttgcggc gcgcttgttg
    15601 ctgagccgtc acggccatgc gacgagcggc attcctggta gccacgctga gcgcatgcgc
    15661 gagcgcggag tcgatgccga tcgatccgtt cctcgactcg agcaaaggtg atggtcaccc
    15721 tgcccgcctc gaagagctcg atcattcgaa gctcgacatg gacgagccgt ccgatctcgt
    15781 gcaggtcgac ggcaagctct actcggtcag cgatcggcac tcgaagattt atcgggtcaa
    15841 gcccaacggc gacgccgacg tgcacatgaa tgtcgacggc accgacctcg aagcgatcgg
    15901 tttcgacccc gaccgcggcg agttcctgat cgcggacgag accaagtcga agatctggac
    15961 gatcgatgcc gccggcgatc gccacgacgc gatcgagctc gacaacgctg acgacggcaa
    16021 cagcggcatc gaaggcatcg tggtcgccgg caacggccac ctctatgcgg tcaaggagaa
    16081 ggatccggcc cgcatctacg agctcgacgc ggacggcacg ctgctcgcca gcaagaagat
    16141 cgagctcgcg gacgatctct ccgcgatcac ctacaacgcc gacgacgacc atctctacgt
    16201 cctctcggat gaggatcgct cgctgttccg cctcgacaag cactggaatg ccgaccgggc
    16261 atggaagctg ccggtcgatc atcccgaagg cgtcgccttc gacggcacga cgctctacgt
    16321 cgtcagcgat ggcgaccacc gcatctatac attcgagctc gtcgactagc aggccggatc
    16381 atggatgcgc gtcgaaccac gcggtgatcg cgggtacggc gggtgcgatc cattcgtgac
    16441 ctgcggcggc gtcgatcacg gtatcgacga catggccctg ctgcgcgagc tcgtcgcgat
    16501 atgcgagcat ctgcgtgacg ggtacgatcg gatcctgcgc accgtgcagg aacaatgtcg
    16561 gtgggtgatc gaccggcagg ttcagcggaa gaaggcaggt cacgccgcat gtcgcgtacg
    16621 acgccgagtg cacggcgagc gcgcgaaaca cgccgggata actcacggcc atccggctcg
    16681 tcatgtagcc gccactcgag atgcccgttg catacagacg cgtggtgtcc agcggaccga
    16741 gcgtgccatt gccaattcca gcgatgatcg cgaggagaaa ggcgtggtcc gacgttgccg
    16801 tccagttgag cgagtacggc ggcacgttgg tctgccacgc gatatcgacg agcggctcgg
    16861 gcgcgatcac cgcgtaaccg ccgtcgagca acgcgcgaat cgtccgcacg agctggaact
    16921 gaccgaacgt cgcgtcgcgc gtcgagtgaa acgcttcctc ggcagagtgg aacgagccct
    16981 ggaagaagaa gaccaccggc cagccattcg gcggcggtgt tcccagcggg gtctgatagc
    17041 gaacactgcg catcacgacg agcgcctgaa atgacgcggt ctcgtagggg cacgacagct
    17101 cgtttgctgt ttcggtgcag cgcgatgcga cggcagcatc gaacgcaggc gacgtcgcat
    17161 cgacgctgga cggtgcagcg tcgggcgcgc tcgacggcgt cgtggacgcg cacgcgactg
    17221 cgagcatcgc gatcacaggg cagacgattt tcgatcgttg catacgaatc ccctcccgtt
    17281 cgtgcggcac gacggccgcg atggatcagc tcagctccgc cacgaccagt gctccctccg
    17341 cataacgtgc tcgcaatacc ttcttgtcga acttgccgac gctcgtcttc ggcacttccg
    17401 cgacaaacga ccagcgctcc ggtagccacc agcgcgcgac acgctcggcg agaaaatcgc
    17461 gcagcgcctc ggccagcacg gtcgagccgg gcttgcgcac gacgcacgcg agcggccgct
    17521 ccgaccaccg ggagtcgggc accgcgatca ccgcgcactc gaccacgtcg gggtgcgctg
    17581 cgagcgcatt ctcgagctcg accgacgaga tccactcgcc acccgacttg atgacgtcct
    17641 tcgcgcggtc ggtgatctga agatagccct cgggatcgag cgaaccgatg tcgccggtgc
    17701 ggagccagcc gtcatcgaac ttctccggcg cgggatcgcg atagtaggac gccgtgatcc
    17761 acgggccgcg gacctcgatc tcgccgacct gcgtgccatc ggccggtagc tcgcaaccgt
    17821 tgtcgtcgac aatgcgcgcc tcgacaccgg agagaatgcg acccgtccgc atgcggtacg
    17881 tcatctcgcg ctcgggcggt gtgcccgtcg gtggaatcgc cagcgcggcg atcgggctcg
    17941 tctcggtcat gccccacgcc tgaatgatcc ggcggccaaa ccgtcgatcg aacgcttcca
    18001 tcagactacg cggcaccgcg gcaccgccgc agacaatccg ctcgagcgac gacagatcca
    18061 tgtcgacgcg atcggcgcat gcgaggagcg agctccagat cgtcggcacg gccgcagcga
    18121 acgtgggacg ctcgctcgcg atgatctgtg cgagcgcgtc cggttgcatg aaccggttcg
    18181 gcatcacgag gtcgctgccg gcaaaccagc ccgcgtatgg caagccccag gcgttcgcgt
    18241 gaaacatcgg cacgatcggc aggatgcggt cgcgctcgcc aatccctagc gagttcgtcg
    18301 tgcacgcggc gagcgagtgc agatacgtgg accggtggct gtagacgact cccttcgggt
    18361 ggccggtcgt gccgctcgtg taacacatcg ccgcggcagc tcgttcgtcg agcgtgggcc
    18421 acgcgaagcc gccgcgctcc gccacaagta gctcgtcgta gcgcaacgcg ctcgggatgg
    18481 cggatgcatc gccgcggccg atcacgatga tgtgcttcac cgtccgcagc tgcggtacga
    18541 gtggcgccaa gaccggcacg agcgagtcgt cgaccaagat gatcgcatcc tcggcgtgat
    18601 cgatgatgta tgcgacctgc tccgggaaca ggcggatgtt gagcgtgtgc agcaccgctc
    18661 ccatcgcagg caccgcgaga tacgcctcga gatgctcctg tgtgttccac gcaaaggtgg
    18721 cgacgcgatc accacgctcg atgccgagcc gagcaagcgc acatgccagc tgctcggcgc
    18781 gcttcgcgac ctccgcgaac gtcgcgcgtc gaaactcgcc gtcaccttcg taggtgacga
    18841 cctcgctatc gccatacacc gcccgcccat gctgaaagat cgcggacagc gtgagcgggg
    18901 tatcctgcat tgttccgagc acgaacgaac gttcactcat tgtatgtaaa gctgtcaaga
    18961 gatgattcat gcgttgacca ttggccggat ctgtgcgaca tagcgaatgt aacgagtgtt
    19021 cactcacccg ctgtttcgag gcgaaccttg acccaacttg cccacgtctc cgctgcccgc
    19081 gtccctgccg ctgaagcgaa gctgccccag ctcgggaaga ccggcaccta ccggcgcatt
    19141 ctcgaagaag cgctgatgtt gttcgcgcag acgggttatc acggcacgtc ggtacgcgac
    19201 atcgcgtctg cggtcggcat ccagatcgcg gcgctgtacg tgcactttcc ttcgaaaggc
    19261 cacgtgctcg cggagctgtg ccgcatcggt cacgaggaac accagcgtgc gctgcgcacg
    19321 gcgttgctcg acgccggctc gagcccggcc gatcagctgg ctgcactcgt gcgcgcgcac
    19381 gttcggcttc acgcggagta cgcgatgctc gcgaccgtcg cgaactacga gctgcacgcc
    19441 ctgccggacg agctcgccgg cgcaaccatg gcgctgcgtc gccagtcaga ggcgctgctg
    19501 atcgaggtct gcgagcgcgg cgaggcccaa aagaaattca cgacgcctga cgtggtgatc
    19561 acggccgcgg cgatcggcgc aatgggcatg cgagttgccc agtggtatcg agccgacttg
    19621 ggcaaaacga tcgacgagat tgcgaacgtg cacgccgagc tcgctctgcg catgttcggc
    19681 gcgcgctgag ctggcgcaga aaggtaccga tgccgatcgc cgagcttccg aacctgtgca
    19741 tgagctacca agaggcgggt agcggtgagc cgatcgtgct cctccatggc ctcggctcgt
    19801 gcgcgcatga ctgggaggag cagattgaag cgtttgcgtc gagctatcgg gtgatcgcgc
    19861 ccgatttgcg cggtcacggc gacaccgaca agctgacgcg ctcgacgatc gccgcgatgg
    19921 ccgaggacgt cgtgcagctg cttgccgagc tcggcatcca gtccgtgcat gtcgtcggca
    19981 tgtcgatggg cggcatgatc gcgttccagc tcgcgctcga tgcgccacag ctcgtgaaga
    20041 gcctcgtgat cgtcaatagc ggccccgatg ccgtgccgcg cacgctgcgc caccgcttcg
    20101 cgatcttcac gcgatttgcg ttgcttcgcg tcgtcggcct gccgcgactc actcgcgtga
    20161 tcgcgaacaa gctgtttcct gcttctggcc aagagatgct gcgtcggcgt tttgtcgaac
    20221 gcgcatcgac caatgatccg acggcataca aagcggctct cgacgcgatc gtgagctgga
    20281 gcgtcgcgga tcggctgtcg tcggtcgtcg cgcccacgct cgtcatctcc gccgatcagg
    20341 actacacgcc ggtctcgttc aaggcggact acgtcgcgaa gctgcccaac gcgcggctcg
    20401 tcgtgatccc ggattcgcgc cacgcgacac cgatggatca acctgctcgt ttcaacgcga
    20461 ccgtgctcga gttcctcgcc ggccgcgagc accgcatcaa cgcagtcgcc ggctgatcga
    20521 gagccggcgg catcgggttc tcgcgcgaat ccgcgcggtt tgcccacgtc gcccctccgc
    20581 gcccatgttt tccgcgagct gcgctcaggg cgcgtcgcgc gcgttgtgac gatcttctag
    20641 ttgcgatcgc cgcaaagatc gtgatagcgg tccggacatg aacgaacgat cgttcacata
    20701 aggatcgcca tgaaaaccgc aagtttggtg gttggtgctg tttgtttctc cgtgcctctt
    20761 tgggcgcaac cgcagccttc gccgcaggcc acgggccaac cgagcccggg ccagacggca
    20821 ccggcaccgc cggcggatca gccctccact cctggggcgg atccgtcggc ggattcgcca
    20881 ccgcccaccg caccaccggc tgactcgccc gcggcttctc cgcccgcaag ctcgccaacg
    20941 aacaacgagc tcgcgcgcat ccgcggcgtg atccgcagcg gcgatcacaa cgagcccgta
    21001 cccggcgcga cgatcatgct cagcggcacg accaacgcgg cgatcagcga gtcggacggc
    21061 agctacctca tcgaaggcgt cgtgcctgcg tcgtacacgc tcgagatttc cgcacccggc
    21121 tatgccgaca cgacgatcgc cgccaaggtc gagccggcca agacggctgt cgtcgacgtc
    21181 aacatcggcg agatgaacgc cggcgaggtc atcgtcgtca ccggctcgcg ctcgccggaa
    21241 aagctgctcg attctccggc gaccatcgag tatgtgtcga cgaacgacat ccggcgtgcg
    21301 accggtgcgt cgtacttgtc agcgctctcg aaggtaaaag gagtcgatta cgccgacgcc
    21361 gggctcggcg accagcggat ctcgatgcgc gggttcacga cgcagttcaa ctcgcgcctg
    21421 cttcagatga tcgacggacg catcgcgcag atgcctggca ccggattgcc gcaaggcaac
    21481 tcgctgccga caacgtcgct cgacatgaag tcgatcgagg tcatcgtcgg gccggcgtcg
    21541 gcgctttacg ggccgaacgc gcacaccggc gtgatcaacg tgatcacgaa gacgccgtgg
    21601 gacgaatccg gtgcgacggt cgcagtccgc ggcggttcgc agaacttgat cgatctcggc
    21661 gcgcgcatcg ccggcaccgt caaagacgac ttcggctgga agctgaacgg tcagttcatg
    21721 cgcgcggagg acttcgcgcc cgacgtgatg cggcccgagc actactacaa cacgccgttc
    21781 ttcgagcgtg acctggtcgg cggtagctac gacgtcaagg gcggcaagct cgacgggacg
    21841 ctctactaca agctgcccga cgactggatc gtcaagagca cctacggctg gtcgtacggc
    21901 gatggcttcg cgatcacgaa caacggccgc aatcacctgc gcggcgtgca gacgcaggtg
    21961 cagaacatcc agctcagtgg caagcacctc tacgcgcagg tgacgcgcac ggcgtccagc
    22021 attggcaatg cgtatccgat cgacggcctc gctcgcgcgg ttgctgcgat gggcgcgacc
    22081 acgctgccgt cacccgacgt cctcgagcag atgcgcaccg cgctcaagct cgtcgacgac
    22141 agtcagctct acgatgccga agcgcaatat cgcgcgacgg ttgccggtgt cgaagcaacc
    22201 accggcgtgc aaggccgcgt ctacctgccc gactccggcg gcacctacct cgcggacgcg
    22261 aacggcgaga acatcaacgc gacggaaatc ggcggctacg gccaggcctc gtacaagctg
    22321 ctcgacgacc gcctgcgcct cgtcggtgcg ctgcgcgtcg atcatcactc ggactacggt
    22381 acgcagctca gtccgaaggc tgcggtcgtc gtcaaggttg ccgagaacca caacctgcgc
    22441 gcgggctaca accacgcgct caagagtccg acgatcctcg agagcaacct gctcatccag
    22501 aacatgttcc gcggcaacaa gaccggctac gtgatcaagg acgacaccgg cacgatcgtc
    22561 gacacgctgg cgccgctgtc acccgagcgc gtcaacagct tcgaagtcgg ttacaagggc
    22621 atcgtcgacg atcgcctgtt catcgacgct gtcgcctacg actcctacta ccggaacttc
    22681 atcagcccgc tgaccgcgac cgcgaacccg gcgatgaaca cgttcgcgta tttcccagac
    22741 ggcacgctcg tcggcgacgg aacaccgacc gccggcgcgc tctacacgta tcgcaacttc
    22801 ggcgccgcgc acgtgcgcgg taccgacgtc ggtgtgagct ggcggccagt cgacaccctc
    22861 gatctctcgt cgagcgtatc cgcgatcgac ctcgtcgact tcacgcagtc aaacccgagc
    22921 gtcgcgccac tcctgctcaa cgtgcccgca ctcaagctca agggctcggc cacgatctcc
    22981 aacctcggca tggccaacta cttcgtgaac ctctccgggc gctggcaatc ggcctacaag
    23041 ttcgagtcag gctactggag cagctcgcgc ttctacgccg acaacgacgg caagctgcct
    23101 gcgcgcttcg tcgccgacat cgccgccggc tacacgttcc cgaagcagaa cctcaagctc
    23161 accgcgacgg tcgcgaacgt cttcgacgat cacaacgtcg atctgctcgg gtcgccgatt
    23221 ccgggccgac tcgcattcgt tcaggtcgag tacgcgtacc agggccttac gttctagcgc
    23281 acggcgtcgg tagactccgc gcatgccgac gacctccctc gaacgttccg gtggcattgc
    23341 cacgctctgg ctcgatcgcg ccgacaagcg caacgcgatg gacgcggatc taatgaaggg
    23401 gctcgcagct gccttcgacg agatcggcgg cgatcgctcc gttcgcgtcg tcgtgctgcg
    23461 cggccgcggc aacgtgttct cgagcggcat cgatcacacg ctgctgacgc aggtgttcgg
    23521 cgcgacgcag tcctcgccgt tcctgcacct gcatcatcag ctgcaagaca cgtttcaccg
    23581 catgagccgc ttgcaccagc cggtgatcgc ggcgatgcac ggtgtctgcg tcggcatggc
    23641 gctcgagctc gcgcttgcag cagacattcg catcgcgagc gaagactgcg tgatgggttt
    23701 gccggagatc gcgttcggca tcgtgcccga cgtcggcggc acgacgcgat tggtgcgcgc
    23761 cgtcggcgaa ccccgcgcgc gcgagctgat cttgacgggc cgactcgtga aggcggcaac
    23821 agcggagcgc tacggcctcg tgcacgagat caccggcaat ggcgatgtgc tcgcccagcg
    23881 cgcgatggta gtcgccgagc aactctccgc gcactcgggc accgcactcg ggctggcgaa
    23941 gacgctgtgc tcggcatcgg cagatgcgga tgcggcgacg agcttccggc tcgagggcgt
    24001 ggtgcagcag accctgctcg cgcagccaga tctgatgacc cggttcccgg cggcgctcgc
    24061 cttcatcaag gcccagctcg cgaatccggg ctgaaaattt gcctctcaga ccgatgtgat
    24121 acgggatggg actgatggcc ctggaagtcc tacgcgacgt gttcggctac gacgcctttc
    24181 gcgggggcca gcgcgaggcg gtcgaggcgg tgctcggcgg gcgcgacgcg ctcgtgctgc
    24241 taccgactgg tgccggcaag tcgctctgct atcaggtacc cgcgatcgtc cgcgcgcggc
    24301 gcggtgaggg cacgacaatc gtgatctcgc cgctgatcgc gctcatgaac gaccaggtgc
    24361 gcgcgctgac cgcgaagggc gtggcggcgg cggcgctgca cagtcagtgc gatgacgccg
    24421 aacggctcga gacgatcgcg cggctgacgc gcggcgagct cgcgctcctc tatatctcgc
    24481 ccgagcgcgc ggtgctcgac ggcttcaagc ggctactcgg ccgcgcgcac attgcgatgg
    24541 tcgcgatcga cgaggcgcac tgcgtgagtc agtggggtca cgacttccga ccggagtaca
    24601 tgcgcctcgc cgagctgcgt gcggtggcca gttcgacgat ggcgaccgcg ccgatgatcg
    24661 cgctcaccgc gaccgcgaca ccgcgcgtga tggacgaaat cgcgaccgcg ctcgagctgc
    24721 gcatgccgac gatcgcgcgc ggcgattttc ggcggccgaa cctcgcgttc gaagtcatcg
    24781 cagcgggcgg cgacgacgcg cgcatcgcgg caacgatcga tgcactggag cgcgcgggca
    24841 tgcgtgggcg cagtggcgcc ggtcgcgcga tcgtctactg cagcacgcgc aagaaggccg
    24901 aagatgtcgc ggcgaagctg aagcaagccg ggttcgcggc gggccactac cacgccgggc
    24961 gcactgcgct ggcgcgagaa cgcgcgcaat cgggattctc gctcggccgc atgcgcgtgc
    25021 tcgtcgcgac gaacgcgttc gggatgggca tcgactatcc cgacgtccgc gtcatcgtgc
    25081 actttcaagc accgggcagc ctcgaggcct act
//
LOCUS       DASUFX010000483         9111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2022894, whole genome shotgun sequence.
ACCESSION   DASUFX010000483 DASUFX010000000
VERSION     DASUFX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022894"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFV99_19360"
     CDS             complement(<1..348)
                     /locus_tag="VFV99_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5061539.1"
                     /translation="MADAPVVRLRDVTKSYGEGAGKTHVLRGVSFDIEKGELIALVGQ
                     SGSGKSTLLNIIGGLDQPDAGEVEVLGIDTLKANDAVRAKMRNESIGFVFQAFNLLDH
                     LSVLENVTIPASFS"
     gene            complement(376..1920)
                     /locus_tag="VFV99_19365"
     CDS             complement(376..1920)
                     /locus_tag="VFV99_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061540.1"
                     /translation="MPRVAGALALVSTGVHAGCVTISPAMRALVIALIVAAGCGGKRQ
                     VGPSGPPSTASAFPAARWVPGTPTYVFAARTLRDAQRAFADGIDTFGMPAGVDAEHVS
                     AKLTQLLQVDLLNAEAMSSIGIDLDGGFALFSAEIDPTLVLHLSNPATLQAFIGKQRE
                     RGMVSQAVTIDGVEVNTAKVASDLSISWALDGDWLWAHATFAPHGDNSNWFAASKGAA
                     AGAATWGAKWDAAQKLATKAGNLIGVFDLRAFTGKLAARIPDFAACARQFEPVNGIGI
                     ALEAEGHWVGGKLAVDVGGSAQTIASNTLMPPPGWNGAAARAPINAQWNLDLKTAANW
                     VQPCMNGGPNLASVIDQFGVRSVRAFAHTLDPDDNSGTGAIALDLTHPRFFEQQLSQI
                     PMRSKFERSRQYGAYKGKHLSVPFVATADYVLDDRVFLAAMGDGMLERAATGPGAANP
                     RDVVFSIDLLPPGLSTDVWTWLFAQAELPNPKRLAVQLQSWADIHLNALLDGPTLVIQ
                     AQGNRR"
     gene            1947..2276
                     /locus_tag="VFV99_19370"
     CDS             1947..2276
                     /locus_tag="VFV99_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061541.1"
                     /translation="MSGVGGPGGIGGPKGPSGPSGLDGPDDVGEVGDVGHVDAARATK
                     AAGELEAMAADIAAGKLTANEAIDRLVSEIAGSDQLGTTERAELKEMLTDLVANDPYL
                     QSLVGRV"
     gene            complement(2541..3176)
                     /locus_tag="VFV99_19375"
     CDS             complement(2541..3176)
                     /locus_tag="VFV99_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061542.1"
                     /translation="MVRALVLVAVALVSCAKTETKQLDTSKIRVTSDARLRTDTVGDG
                     KFTDLATFVLVEAENTSEQGAYVTLGGELTDEAGNRVGQLRAQSLWVPAGELRTFALV
                     DRQRKPRPDAKAAKIYVRGAKLGVAPPAHVDSIRQVDDADKVVVQGTLVNEGKRGGNI
                     MVIATFFDANQKPMTRPFSLLWVDAKGSLPVQFAGPPGSKRGTIFIGDVAY"
     gene            complement(3230..3523)
                     /locus_tag="VFV99_19380"
     CDS             complement(3230..3523)
                     /locus_tag="VFV99_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061543.1"
                     /translation="MKSEVILEVLENCAEQLGIRVSYEPLQTSGIQTGLRGGLCRVKG
                     AYRVIIDKRATGEERVTTLATALAGFDTTELELPQKVRDLLRMYEGTGPRRAA"
     gene            complement(3582..5156)
                     /locus_tag="VFV99_19385"
     CDS             complement(3582..5156)
                     /locus_tag="VFV99_19385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5061544.1"
                     /translation="MLTPPSEAAQLAALRELAAELPFAQSETVVVERVLGVLGELFPG
                     RALAVRVLDVRTRDPARAYVRSATLRESVTTEGVTITQEALNRARLKSAVAASARVVV
                     RERWDSPFQGMATGFAVPLAAGGELYGVLDIGYPPGDEARDADQQAVMSIAQGFAFAL
                     RTIRLQEDAIGLRDYQARLLDSANALILGIDRTWRITVCNRALLELTGTTRDEVLGRD
                     VRDFISGEQRQQLTTAFSAALTGQHHGAFTVMLPTRRGPVRSVWSIAPVGRAGQPGGP
                     VEAVVAIGQDQSKIDMLQQQIVRAERLATLGELAAGVVHELNNPLTSITVYAEYLVRK
                     LEAQGTEQADLEKLRRIGASAQRILRFSRDLVQYARPSGKETESVDLAAVVRQSVSIC
                     EHLVERGGVTLGVEVDPELPVVQAVAGQLEQVLINLITNAVHAVENGGKVVVRAQVES
                     PSEIAIEVADSGPGVPEHDRDKIFEPFFTTKADGKGTGLGLPIVRNIVDQHRGVISVK
                     RSDLGGAAFRVVIPIV"
     gene            complement(5156..7261)
                     /locus_tag="VFV99_19390"
     CDS             complement(5156..7261)
                     /locus_tag="VFV99_19390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5061545.1"
                     /translation="MEAQGIVVLGGSDAERAALVSTLNGSGLTARGMAKLTAADGIPA
                     LIVIAGDHVIELVGDARQVPALSDTPILALAPQMSQTTVAGVLAAGATDVVRWPAPSV
                     LLVARCRNLLKLSRRDAANAQALTKINEVLTADGDDSEALIQVLQITASVLGFDRASL
                     VAHIDGSDHAFVIAASDAEPMARFTLAIQDYPEVLETIRSGQPLMIDDVLTHPVTSRI
                     GDALSARGIRGVAVFPVQWRARSLGVILLRKRMPGVGHIGARGVELGRLITSITAAHL
                     RHGAVLESLRDQTHRISRARYEAERRLRGIDSLKEHFEAGADGVVVLDDAGRILFVNR
                     AAERITGFARDGLLGSELVDMLPTEQRSQITDAISRVLAGVNVDAFDLELSTTSGSPV
                     WVSVSTSTVLAGSGAVILAFRDVTAERALELELRSTKEFLERLIDSTVDAIIAADFKG
                     QIILFNQGAERLFGFHARDVIGKKPVWELYDTGGARQIMRMLRSTSYGGVGRLEQTRR
                     EVRIASGETVPVSMSASIVYEGEREVATVGILTDLRERIRMEQRLLDAQQKLQNSEKQ
                     ALVAELAGAAAHELNQPLTSIIGYAQLIQRQSEKEAAHLRAVGIILSEAERMAGIVKK
                     IGKITKYETTDYVGSAKMLDLDRAAADSADAIPIETVEAEESKTGEFTAVAPVRESDA
                     NMPSLGDLTELADRQGVKR"
     gene            complement(7283..8755)
                     /gene="larC"
                     /locus_tag="VFV99_19395"
     CDS             complement(7283..8755)
                     /gene="larC"
                     /locus_tag="VFV99_19395"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX5061546.1"
                     /translation="MKGLHLHIDCPAGAAGDMVLGALIDLGVPIDVIGDALDAIGAGR
                     GRLRVQRVVKHGIAATDLKVDTSGPILDERVPRVMDRLAPASGPERAGAPRQPHSHAD
                     DHRHAAPRQPHTHADSGKVRARADSSSSGAFVAIKFRAETSTRHVTVDEHGDKTEEVH
                     EHGGENDHAHYHYAEIRWRISNAQLPPGTRTRALDIFDRLARAEAKLHGTSLDDVTFH
                     EVGAIDSIVDVVGTAAALDWLGPASVTCASVAMGHGTLVCAHGVLPVPAPAALEVLRE
                     AGGVMTDGGVATELCTPTGAAILAATVTHWTSAPTARPVAVGWGAGDLDLPDRANVLR
                     VVTLVPQVALAESVWQIDANIDDMSPELSGAATDALFAAGAVDVWWTPITMKKGRPAL
                     TLSVLAETAKRDDVINTILRETTTIGVRYSQLQRTVLARQMVEVDTRYGKIPVKVAMD
                     GNIVVNAAPEFEACASAARQHGVPVKLVFSAALAAYDAHR"
     gene            complement(8752..>9111)
                     /locus_tag="VFV99_19400"
     CDS             complement(8752..>9111)
                     /locus_tag="VFV99_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX5061547.1"
                     /translation="EEAALVAEFLGAPVTRISDVGVAGLHRLLARLDDIRKTQVVIAV
                     AGMEAALPSVLGGLIDRPMIAVPTSIGYGVSIDGLVALGALLSACAPGISVVNIDNGV
                     GAAVAAVKIARLAKAGA"
BASE COUNT         1469 a         3104 c         3059 g         1479 t
ORIGIN      
        1 cgagaacgac gccgggatcg tgacgttctc gagcacgctc aggtgatcga gcaggttgaa
       61 cgcctggaac acgaaaccga tcgattcgtt gcgcatcttc gcgcgcaccg catcgttcgc
      121 cttcagcgtg tcgatgccga ggacttcgac ctcgcctgcg tcgggctgat cgaggccacc
      181 gatgatgttc aagagcgtcg acttgcccga gcccgactga ccgacgagcg cgatcagctc
      241 acccttctcg atgtcgaagc tgacgccacg caatacgtgg gtcttgcccg cgccttcacc
      301 gtagctcttg gtgacgtcgc gcaaacgaac gacaggagcg tctgccacgg ccggacccta
      361 gcacatcccc aagcgttacc gccggttgcc ctgcgcttgg atgacgagcg ttggcccgtc
      421 gagcagcgcg ttcaggtgaa tgtctgccca gctctggagc tgcactgcga gccgctttgg
      481 attgggtagc tcggcctggg cgaagagcca cgtccacacg tcggtcgaca gcccaggcgg
      541 cagcagatcg atcgagaaca ccacatcgcg cggattcgca gcgccgggtc ccgtggcagc
      601 gcgctcgagc atgccgtcgc ccatcgcggc gaggaacacg cgatcgtcga ggacgtagtc
      661 agcggtcgcg acgaacggta ccgacaggtg cttgcccttg tacgcaccgt actggcgcga
      721 gcgttcgaac ttgctgcgca tcgggatctg gctcagctgc tgctcgaaga accgagggtg
      781 cgtgaggtcg agcgcgatcg cgcccgtgcc cgagttgtca tcgggatcga gcgtgtgcgc
      841 gaaggcgcgg accgagcgca caccgaattg gtcgatgacg ctggcgagat tcggtccacc
      901 gttcatgcac ggctgcaccc agttcgcggc cgtcttcaag tcgaggttcc actgcgcgtt
      961 gatcggcgcg cgcgccgcag cgccgttcca gccaggcggc ggcatgagcg tattgctcgc
     1021 gatcgtctgc gccgagccgc cgacgtcgac ggcgagcttg ccgccgaccc aatggccttc
     1081 cgcttcgagc gcgatgccga tgccgttgac cggctcgaac tgacgcgcgc atgcggcgaa
     1141 gtcggggatc cgcgccgcga gctttccggt gaacgcgcgc agatcgaaga cgccgatcag
     1201 gttcccggct ttcgtcgcga gcttctgcgc tgcatcccac ttcgcgcccc acgtcgcggc
     1261 gcccgctgcg gcgcccttgc tcgcagcgaa ccagttcgag ttgtcgccgt gtggcgcgaa
     1321 cgtggcatgt gcccacagcc aatcgccgtc gagcgcccag ctgatgctga gatcgctggc
     1381 gaccttcgcg gtgttgacct cgacaccgtc gatcgtcacc gcctggctga ccatgccgcg
     1441 ctcgcgctgc ttgccgatga acgcctgcag cgtggccgga ttggacagat gaaggaccag
     1501 cgtcggatcg atctccgccg agaacagcgc aaagccaccg tcgagatcga tgccgatgct
     1561 cgacatcgcc tccgcgttca atagatcgac ctggaggagc tgcgtcagct tggcggacac
     1621 gtgctcggca tcgacgccgg ccggcatgcc gaacgtgtcg atgccgtcgg cgaacgcacg
     1681 ttgcgcgtca cgcagggtgc gtgctgcgaa cacatacgtc ggcgtacctg gcacccagcg
     1741 cgccgccggg aatgcagacg cggtggacgg cggaccggaa gggccgacct gccgcttgcc
     1801 accgcacccg gcggcgacaa tcaaagcgat cacgagggct cgcatcgccg gcgatattgt
     1861 cacgcaaccg gcgtgaacgc cggtcgagac aagcgccaac gcgccggcaa cgcgcggcac
     1921 gaaaccatgt atcctgcgag aagcacatga gcggagttgg tggacccggc ggaatcggcg
     1981 gcccgaaggg ccccagtggc ccatccggcc tggacggtcc ggatgacgtc ggtgaggtgg
     2041 gcgacgtcgg ccacgtcgat gctgcgcgcg cgacgaaggc ggccggcgag ctcgaggcca
     2101 tggcggccga cattgccgcc ggcaagctca ccgcgaacga ggcgatcgat cggctggtca
     2161 gcgagatcgc gggcagcgat cagctcggca ccacggagcg cgccgagctc aaagaaatgc
     2221 tgaccgacct cgttgcgaac gatccgtatc tgcagtcgct cgtcggccgc gtctaaccgg
     2281 ccaagccgcc gtccccacca agaccgagcg tcacgctgct gcgcccgacg ctcgcgcgcc
     2341 agagttaacc gccgagacgc cgcgggcgcc aagatcggag atcgatctgg atcgcgctct
     2401 gctcgcacgc acgccggcgc gaacacgacc tttaggcgcg gagtgctgtc gcctgaacgc
     2461 gctccgacct aagagatcct ctgggcttgg cgtgtgcggc gccttgtatg tcgcaaccaa
     2521 cacagttgct tggcggttgc ctaatacgcc acgtcgccga tgaagatcgt gccgcgcttg
     2581 ctgcctggcg ggccggcgaa ctggactggg aggctgccct tcgcgtcgac ccacaacagc
     2641 gagaacgggc gcgtcatcgg cttttggttc gcatcgaaga acgtcgcgat gaccatgatg
     2701 ttgccgccgc gcttgccctc gttgacgagc gtgccttgca cgacgacctt gtccgcgtcg
     2761 tcgacctggc gaatgctgtc gacgtgcgcg ggcggcgcga cgccgagctt ggcgccacgt
     2821 acgtagatct tcgcagcctt cgcatccggg cgcggcttgc gctgcctgtc gacgagcgcg
     2881 aacgtgcgca gctcgcccgc gggaacccac agcgattgcg cgcgcagctg gccgacgcga
     2941 ttgcccgctt cgtcggtgag ctcgccgccg agcgtgacgt aagcgccctg ctcgctcgtg
     3001 ttctccgcct cgacgagcac gaacgtcgcg aggtcggtga acttgccatc accgacggtg
     3061 tcggtgcgca gccgcgcatc actcgtcacg cggatcttgc tggtgtcgag ctgcttggtc
     3121 tcggtctttg cacacgagac cagcgcgaca gcgaccagga ccaatgcgcg caccacccgc
     3181 gcagtctagc gcatcgccac cgcggcgcag cgcgacgcgg cacagatgcc tacgcagcgc
     3241 gacgtggtcc agtgccttcg tacatccgca ggagatcgcg gaccttctgc ggcagctcga
     3301 gctcggtcgt atcgaaaccg gcgagcgcgg tggcaagcgt cgtcacgcgc tcctctcccg
     3361 tcgcacgctt gtcgataatg acgcggtacg cgcctttaac gcggcacagc ccgccgcgca
     3421 gaccggtctg gataccgctc gtctgcagtg gctcgtagct gaccctgatg ccgagttgct
     3481 cggcgcagtt ctcgagcacc tcgagaatga cttctgactt cattgtctgg gccccacact
     3541 accagcgtgg atccagcgcg caactcttct gacccgcgtt gctaaacgat cgggatcact
     3601 acgcgaaatg ctgctccgcc cagatcggag cgcttcacgg agatcactcc gcggtgctga
     3661 tcgacgatgt tacgcacgat cggaagaccg aggccggtgc ccttgccgtc ggctttcgtc
     3721 gtgaaaaatg gctcgaagat cttgtcgcga tcgtgctcgg gaacacccgg cccggagtcg
     3781 gcgacttcga tcgcgatctc gctcgggctc tcgacctgcg cgcgcacgac gactttcccg
     3841 ccgttctcga ccgcgtggac cgcgttcgtg atcaggttga tcagcacctg ctcgagctgg
     3901 ccggcgaccg cctgaacgac gggcagctcg ggatcgacct cgacacccag cgtgacgccg
     3961 ccgcgctcca cgagatgctc gcaaatcgac accgactggc gaacgaccgc ggcgagatcg
     4021 acactctcgg tctccttgcc actcgggcgc gcgtactgca cgaggtcgcg cgagaagcgg
     4081 aggatgcgtt gtgcgctcgc accgatgcgg cggagcttct cgagatcggc ctgctcggtg
     4141 ccctgcgcct cgagcttgcg cacgaggtac tcggcgtaga ccgtgatcga cgtcagcggg
     4201 ttgttgagct cgtgaacgac gccggctgcg agctcgccga gtgtggcgag ccgttcggcg
     4261 cgcacgatct gctgctggag catgtcgatc ttcgactggt cctggccgat cgcgaccacg
     4321 gcttcgaccg gaccgcctgg ctggccagcg cggccgaccg gcgcgatgct ccacacgctg
     4381 cgcaccggcc ccctgcgcgt cggcagcatc accgtgaacg cgccgtggtg ctggccagtc
     4441 agcgctgccg agaatgcggt cgtgagctgc tgacgctgct cgccggagat gaagtcgcgg
     4501 acatcgcggc cgagcacttc gtcgcgcgtc gtgccggtca gctcgagcag cgctcggttg
     4561 cacaccgtga tgcgccaggt gcgatcgatg ccgaggatga gcgcgttcgc gctgtcgagc
     4621 agccgcgcct ggtagtcgcg tagaccgatc gcatcctctt gcagccgaat cgtgcgcagc
     4681 gcgaacgcga agccctgcgc gatcgacatc accgcttgtt gatcggcgtc gcgtgcttcg
     4741 tctcccggcg ggtagccgat gtcgagcacg ccgtacagct caccgccggc agcgagcggc
     4801 accgcgaagc cggtcgccat gccctggaac ggcgaatccc agcgctcgcg gacgaccacg
     4861 cgcgcactcg cggcgacggc gctcttgagc cgcgcgcgat tgagcgcttc ttgcgtgatc
     4921 gtcacacctt cggtcgtcac cgactcgcgc agcgtcgcac tgcgcacgta cgcgcgcgcc
     4981 gggtcccgcg tgcggacatc gagcacgcgc accgcgagcg cacggcccgg gaacagctcg
     5041 ccgagcacgc cgagcacgcg ctcgacgacg acggtctcgc tctgcgcgaa tggaagctcg
     5101 gcggccagct cgcggagtgc ggcgagctgc gcggcttcgc tcggcggcgt gagcatcagc
     5161 gcttcactcc ctggcgatcg gccagctcgg tgagatcgcc gagcgacggc atgttggcgt
     5221 ccgactcgcg gaccggcgcg acggccgtga actcgcccgt cttggactcc tcggcttcga
     5281 ccgtctcgat cggaatagcg tcggcactat cggcggcagc gcggtccaaa tccaacatct
     5341 ttgcggatcc cacgtagtcg gtcgtttcgt atttggtgat ctttccgatc ttcttgacga
     5401 tgccagccat gcgctcggct tcagacaaga tgatacccac agcgcggagg tgtgcggcct
     5461 ctttctcgct ctgacgttgg atcaattgtg cgtacccgat gatcgaggtc agcggttgat
     5521 tgagctcgtg cgcagcggcg ccggccagct cggcgacgag cgcttgtttc tccgagttct
     5581 gtagcttttg ctgtgcgtcg aggaggcgct gctccatgcg gatgcgctcg cgcaagtcgg
     5641 tgaggatgcc gacggtggcg acctcgcgtt cgccttcata aacgatcgac gccgacatgc
     5701 tgaccggcac ggtctcgccg ctcgcgatcc ggacctcgcg ccgcgtctgc tcgaggcggc
     5761 cgacaccgcc gtagctcgtc gaccgcagca tgcgcatgat ctggcgcgcg ccaccggtgt
     5821 cgtagagctc ccagaccggc ttcttgccga tcacgtcgcg ggcgtggaag ccgaacagcc
     5881 gctcggcacc ctggttgaac aggatgatct gacccttgaa gtcggcggcg atgatcgcgt
     5941 cgaccgtcga gtcgatgagc cgctcgagga actccttcgt cgaccgcagc tcgagctcga
     6001 gcgcgcgctc ggcggtgacg tcgcggaacg cgaggatgac cgcaccgcta ccggcgagca
     6061 ccgtcgaggt cgagaccgag acccacaccg gcgagcctga cgtggtcgac agctcgagat
     6121 cgaacgcgtc gacgttgacg ccggcgagca cgcgcgagat cgcgtcggtg atctggctgc
     6181 gctgctcggt tggcagcatg tcgacgagct cgctgccgag cagaccgtcg cgtgcgaagc
     6241 cggtgatgcg ctcggcggcg cggttgacga acaggatgcg gccggcatca tcgagcacga
     6301 cgacaccgtc ggcgccggcc tcgaagtgct ccttgagcga gtcgatgccg cgcagccggc
     6361 gctcggcctc gtagcgcgca cgcgagatgc gatgggtctg atcacgcagg ctctcgagca
     6421 ccgcgccatg gcgcaggtgg gcggcggtga tgctcgtgat cagccggccg agctcgacgc
     6481 cccgcgcgcc gatgtgaccg acaccgggca tccgcttgcg gagcaggatc acgccgagcg
     6541 accgcgcgcg ccactgcacc gggaatacgg cgacaccgcg gatgccgcgc gccgatagcg
     6601 catcgccgat ccgcgacgtg acgggatgcg tgaggacgtc gtcgatcatc agcggctgac
     6661 cgctacgaat cgtctcgagg acctccggat agtcctggat cgcgagcgtg aaccgcgcca
     6721 tcggctcggc atcactcgca gcgatcacga atgcgtggtc ggagccgtcg atgtgcgcga
     6781 ccagtgatgc gcgatcgaag ccgagcaccg acgcggtgat ctggaggacc tggatcagcg
     6841 cctcggagtc gtcgccgtcg gcggtgagca cctcgttgat cttcgtcagt gcctgcgcgt
     6901 tcgcggcgtc ccgacgcgac agcttgagca gattgcggca tcgcgcgacg agcagcacgc
     6961 tcggtgccgg ccagcgcacg acatcggtcg cgcctgccgc gagcacgccg gccaccgtcg
     7021 tctgcgacat ctgcggagcg agcgcgagga tcggcgtgtc actgagcgca ggaacctggc
     7081 gcgcatcgcc gacgagctcg atgacgtggt cgccagcgat gacgatgagc gccgggatgc
     7141 cgtcggcggc ggtcagcttc gccatcccgc gcgccgtgag cccgctaccg ttgagcgtcg
     7201 acaccaacgc ggcacgttcc gcgtcgctgc cgccgagcac gacgatgcct tgggcctcca
     7261 cttcgggtca ggctaccacg cgctaccgat gggcgtcgta ggccgcaagc gccgccgaaa
     7321 acaccagctt gaccgggacg ccgtgctggc gcgccgccga cgcgcaggcc tcgaactccg
     7381 gcgccgcatt gacgacgatg ttgccatcca tcgcgacctt gaccggaatt ttgccgtagc
     7441 gggtgtcgac ctcgaccatc tgacgagcga gcacggtgcg ctgcagttgg ctgtaacgca
     7501 cgccgatcgt cgtcgtctcg cggaggatcg tgttgatgac gtcgtcacgc ttcgcggtct
     7561 cggcgagcac cgacagcgtg agcgccggcc tgcccttctt catcgtgatc ggcgtccacc
     7621 acacgtcgac agcacccgca gcgaatagcg cgtcggtcgc cgcgccggag agctcggggc
     7681 tcatgtcgtc gatgttcgcg tcgatctgcc agacgctctc ggcgagcgcg acctgcggca
     7741 cgagcgtgac gacgcggagg acgttcgcgc gatcgggaag atcgagatcg ccggcgcccc
     7801 acccgaccgc aaccggccgc gccgtcggtg cagacgtcca gtgcgtcacg gtcgcggcga
     7861 gaatcgctgc gccggtcggc gtgcacagct cggtcgcaac gccgccgtcg gtcatcacac
     7921 cgcccgcctc gcgcagaacc tcgagtgcag caggcgccgg caccggcaac acgccatgcg
     7981 cgcagaccag cgtgccgtgg cccatcgcga cgctcgcaca cgtcacgctc gccgggccga
     8041 gccaatcgag ggcggccgcg gtaccgacga cgtccacgat cgaatcgatc gcgccgacct
     8101 cgtggaacgt gacgtcgtcg agcgacgtac cgtgcagctt cgcttccgca cgtgcgaggc
     8161 gatcgaagat gtcgagcgcg cgagtgcgcg tgcccggcgg cagctgcgcg ttcgagatcc
     8221 gccagcgaat ctcggcgtag tgatagtgcg cgtggtcgtt ctcgccgccg tgctcgtgaa
     8281 cttcctcggt cttgtcgccg tgctcgtcga cggtgacgtg tcgcgtggag gtctcggcgc
     8341 ggaacttgat cgcgacgaac gcgcccgagg agctagagtc cgcccgggct cgcaccttgc
     8401 cggagtcggc gtgggtatgc ggttggcgcg gcgccgcatg gcgatgatcg tcggcgtggc
     8461 tgtgcggctg gcgcggcgct ccggctcgct ccggcccaga ggccggagcg agccgatcca
     8521 tgacacgggg gactcgctcg tcgaggatgg ggccgctcgt gtcgaccttg agatcggtgg
     8581 ccgcgatgcc gtgcttgacc acgcgctgca cgcggagccg gccgcggcca gcgccgatcg
     8641 cgtcgagcgc gtcgccgatc acgtcgatcg gtacgccgag atcgatcaac gcaccgagca
     8701 ccatgtcgcc ggctgcaccg gcgggacagt cgatgtgaag gtgcaagcct ttcatgcgcc
     8761 cgccttggca agccgagcga tcttgaccgc ggcgacagcg gcaccgacgc cgttgtcgat
     8821 gttgacgacc gaaatcccgg gcgcgcacgc gctgagcaac gcaccgagcg cgacgaggcc
     8881 gtcgatcgag acgccgtaac cgatgctggt cggcacagcg atcatcggtc gatcgatcag
     8941 gccgccgagc accgacggca acgcggcctc catgccggcg accgcgatca cgacttgcgt
     9001 cttgcgaatg tcgtcgagcc gcgcgagcaa gcgatgcagg ccggcgacgc cgacgtcgct
     9061 gatccgtgtc accggagcac cgaggaactc ggcgacgagt gccgcttctt c
//
LOCUS       DASUFX010000484        12817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1049905, whole genome shotgun sequence.
ACCESSION   DASUFX010000484 DASUFX010000000
VERSION     DASUFX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12817
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049905"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFV99_19405"
     CDS             complement(<1..209)
                     /locus_tag="VFV99_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5061548.1"
                     /translation="MSFTQREARALIRKAVGRVPEGESIRHLNIMPMMDMMTILLVAF
                     IFQMATSALELTAGTVELPRTTTDAP"
     gene            complement(206..1024)
                     /locus_tag="VFV99_19410"
     CDS             complement(206..1024)
                     /locus_tag="VFV99_19410"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5061549.1"
                     /translation="MGNLSAQRVRSKTRHAVRRREDQIEQEEIESGELNLIPYLDMVT
                     NLMLFLLFSISAGIIFTQIDTSLPDKAPPATTTQTNPNQNPDEQPLKLVVSITRDRMF
                     LWSISGLEGTLAAPHATFPRSGRDGEQCDGNYMCESNFCRNTTQKCEPSPAKEVPLPV
                     FDYRGLSASLFEIANRRYAGKQRKADTYQIILMADGAIPYNTVASVMSAMRCKMPEFG
                     KDTDGCALPTEDPELKKAKDPISPDKRLYDTTRATYDPKTMALFSDILFSSGFE"
     gene            complement(1046..1717)
                     /locus_tag="VFV99_19415"
     CDS             complement(1046..1717)
                     /locus_tag="VFV99_19415"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5061550.1"
                     /translation="MSWLSDVFQKGGPFFIVNTFFLAVVIGLIVERAIYFLGRGHLNA
                     KAFLEQLRKLLSANNVDRAKKLCDATTAPVARVAKAGLNRLHRGEAAVAQAMEETMTD
                     TLPEVKTRIGALWSLANICTLVGLLGTITGLIRTFGAIGEANPAEQRERLSTGISEAM
                     YNTAFGLSIALICMVGHLLLSAAMKKVVADLEAFSLRFENLLADGGTAAAASRAPAAP
                     TEDVE"
     gene            complement(1857..2354)
                     /locus_tag="VFV99_19420"
     CDS             complement(1857..2354)
                     /locus_tag="VFV99_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061551.1"
                     /translation="MRAVLLAALLVSGCRIDLDHADVDANTSGRSCKVSMAAVCKEAD
                     SHSDFTWIKNKIFMTNCFGSSCHSGSTASGKLDLTDDPYTSLMGSAGTGTASNLDPAH
                     QRVVAGDPASSYLFFIIHGVEMNTAGFAEPPGNVGYMPMSNSTLCCQKIDAIDRWITA
                     GAMNN"
     gene            complement(2432..5116)
                     /locus_tag="VFV99_19425"
     CDS             complement(2432..5116)
                     /locus_tag="VFV99_19425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5061552.1"
                     /translation="MAAPIRPKQGSREQRRRADIRRGWIYIALGFIVTVAGAVNTAVS
                     VFADGHISFTDVLPIVAGLITMFVGRSLWRRWRKGLGTASVLRIVLLLGLYGVSLAAA
                     TLYFAIKTINEDLPQDLTKLLEYQPYRKSIVLSSDGEEIGAFSIENRRIIQLERMPPH
                     VPAAFLSAEDRRFYKHPGFDLAGIVRAAWKNFRSTGIKQGGSTITQQIIKQTLLADEE
                     GDLSEVGFTPQQLAANKKTQKYKRKMKEVILAYRLERELTKAQILSIYLNHVYLGHGA
                     YGVGAAAETYFGKEVEDLTIAEAAMLAGLVASPTKYAPHRNMQLARERQRYVLGHMRE
                     DQYISDAEYNQALAEPIALVDEGDLNHLASPYFVEHVRKLATKRYGNTELFKGGLRFY
                     STLDMTMQAAAESALKKGLESLDRRLGFRGPIGNVATKNRGAWTGGPAHPLSGGTDDT
                     SALADQLLPEQRYGAMIVELTKKGGVIVDLGPKRLPLDDKDAKDVLAWHPKKKDNTID
                     YDKRIQLGDLLAVRLNAEGTGATIAQRPALQGSLVAMEPSTGRVVALVGGYDWTASQF
                     DRATQAHRQVGSSIKPFIYSSAIEAGKTPVDTMYDGPFAVQTATGTWMPANYDNKYMG
                     NVTLMTALAYSLNTISVQIATQVGLDRIIEILRGFGITSDIPRHISISLGTPDITPIE
                     IATGYAGIANGGRRVTPRFFDLVTSTGGTVVEDLRNPPPGPQVISPEVAYVVINMMKG
                     VVSRGTAKYATTIGRPTAGKTGTSANYKDVWFTGFTTDLLCSVWIGRDDSTPIGDKIT
                     GGGVAVPIWVDFMTKAHPRTKVRDFPPPPNVTFARVEPWSGDPASPHTDGSSVWMPFV
                     RGTLPYRFLASLPVRAFDDLTPTPTVPRPAKCNSLDCL"
     gene            5132..7819
                     /locus_tag="VFV99_19430"
     CDS             5132..7819
                     /locus_tag="VFV99_19430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061553.1"
                     /translation="MRSRLPPHVFAVLAMCVASAGAQGPAPTQATAPAPDIDEPPVAA
                     KAAPSDAKLSASAVPDYTAGPIKIAVTPFENHVPNGKSLEWVVAEAPFEIAEKAENIL
                     DLEAVNAPLYVPGERVPAESDTVADFAKKMGAKLVITGWYDRLGEQLRLVVLVWQADG
                     ANAKVVGEAQRIGAVGSYHKTLGDAMAEAFGEAGIPVDNARTERLSRTLSNDIYPVFM
                     MGRGLGNFSGALAAMSSVFGTGAGSGSAATGPDLKAAEHDLERAVFLDPKLFEAQRLL
                     GELYMQTAAGDPKLINKATGKFNYAADLAPDDAPSLRAAAFAMARAQKWEVALDLFRK
                     LVTRRPWDLEARYQLGAALWQTGNAKAAERQLEQVTAKQPDHLPARRVLVLIHSAHND
                     TPRLIRELEAIAQRAPDDLDVKADLATAYGAMKQWPKMIGALEEIEKQRPNDLALLVR
                     LGDAYRKHGTLDKALAAYQRAGHIDRDVSLPGFAAAQALFDANRLPEAIKAYQLLQNG
                     SDRAAAEHALGTIAYLQGRADEAAWYMRQSARSAPRNLTMRRAVIAAELLRKDAPAAR
                     VQLDYALATWPHDGFLHYFSGVAYHLAGDDTQARTELTTALAETPGLPAATAALAALG
                     ARSPLAVDYKPELVRPWGDGDALQAAIESYAATSTAMARVRADYQNQVLALLGALGQG
                     PKTTVKAGSVKTCPVTQVAKPWETAQKSLQKYAQLGAELETTYRFIARHDEVGMTASL
                     LPNARTQVAGAKKSYRTALADMGELRSEWTRGLTPELRVVGCSDKLLQAALKDPMRYR
                     VVQEDKPEITGAHQAPRPKPRVTFFVDNTRCADTVDVWIDGEHIGQVAPGRRSALVTD
                     GGARTLCLLLPGGAQCGDRGTMRQAYLHDGWSTTLYCPK"
     gene            complement(7939..8553)
                     /locus_tag="VFV99_19435"
     CDS             complement(7939..8553)
                     /locus_tag="VFV99_19435"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5061554.1"
                     /translation="MAKLTITTFITLDGVMQAPGGREEDTSGGFQNGGWLVPHVDQVF
                     GEFMTDVFSRVDAFLLGRGTYRIFESYWPKMTDPNDPVASKLNNLPKHVVSRTLDRTT
                     WNNSVIVRNLADEIGAIKARYTGELQVHGSHGLCQSLINGGYVDEINTLVFPVAVGSG
                     KRLFETGTTPTAFKLASSRTTQNGIVIATYQRTGAPTYGTFAAP"
     gene            8661..9581
                     /locus_tag="VFV99_19440"
     CDS             8661..9581
                     /locus_tag="VFV99_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061555.1"
                     /translation="MHLPHLAFGSPRLLPKPEKVPGRVVVLDIAFAATVGTSVSFVLT
                     TKPFIDGLGDRLAAWVDHHDHERHADFASDPRFVLATKAQHGACPEMVTPDLVRQTGP
                     IDSILTHVDLDGLYAAAKWIMAGKEPYAGADDDARAVDTRVGTPGPIANRIDRALRAK
                     FRDDQLKRAVVHYLVGGMKPGPHDDVITEAAAEFDRRDARTQELARRFTIRGRVAVVD
                     TAGAAGQFDKTDLLLAGQAKAQVSIVRDSGMLTIAAPFDSGWDFVTMFELGGGMPTRV
                     TIPEQKLDDVIGKINDAPAPTPRVGSTPDD"
     gene            9657..10292
                     /locus_tag="VFV99_19445"
     CDS             9657..10292
                     /locus_tag="VFV99_19445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5061556.1"
                     /translation="MALQLGDEAPNFTAETTEGQVNFHEWIGNSWAVLFSHPKDFTPV
                     CTTELGRVSALKGEFDKRNVKVIGLSVDPLDDHKRWVGDIKETQGHALNFPLIADPDR
                     KVANLYGMIHPNANDTLTVRSVFVIGPDKKIKLTLTYPASTGRNFDELLRVIDSLQLT
                     AKHKVATPVDWKQGQDVIIVPSVSDADAKNMFPSGWKTLKPYLRVVPQPKS"
     gene            10292..11284
                     /locus_tag="VFV99_19450"
     CDS             10292..11284
                     /locus_tag="VFV99_19450"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5061557.1"
                     /translation="MTRAALVAVQLEIGADVLVTPEAYRLHIEAEGERALAEGGGEKA
                     DARLVVYPEIAGHLALYALAPHVARRAKTLGGALAAAAARRPLDVIRGVATTRLLDAR
                     HAVLAALAPDGERWWKSVFGPLARRLDAYIVAGSYLRLGSDGALTNASLLFAPDGRLL
                     ATTDKVNLVPGMEDGARGALALARGDARIPIVETPLGRVATLVCYDGFREPHTSTERF
                     VELGPLLAERGGVDIVANPAANPWPWKGPWKFNEPGESLVREEQWRLEGLTGSLTKSA
                     FAKYGITAHLVGKVLDLAFDGASEIVACEGGVTRVLARAEHHDRGGHVTALVTC"
     gene            11294..12256
                     /locus_tag="VFV99_19455"
     CDS             11294..12256
                     /locus_tag="VFV99_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEX5061558.1"
                     /translation="MGERRPLAVRQPGAPGVPEAPSAADEVAELLLEAYLDPRAAEMQ
                     HIGSYELPSDGQVERVLEMCRALLFPGYAGPDIERMEKGSLRELVGLRLEELRYALHR
                     QVYRASHHRKQQLLGRNELECAECTRNADAITDRFIARLPELRAMIRLDLRAAYESDP
                     AATGIDEILLTYPGAYAVTVYRIAHALLKEGAVIVPRMMTELAHRRTGIDIHPGAEIG
                     HSFFIDHGTGVVIGETTLIGDRVRIYQGVTLGALTVPQGAARPEPGRRRHPTIEDDAV
                     IYANATLLGGDTVIGKGAVIGGNAFITASVAPGTKISGSSRTQK"
     gene            12313..>12817
                     /locus_tag="VFV99_19460"
     CDS             12313..>12817
                     /locus_tag="VFV99_19460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061559.1"
                     /translation="MTDDVQARPRTNTRLKGEAPPGLALSGEEGRKSIGRYEVVKTIG
                     KGAMGVVYKARDPLLDRIVAVKTIMSPQQQGRRVRSAFLERFQREAKAAAKMSHPAIV
                     TIFDVGVDEESGAPFMVLEYLPGESLADRLDRVRLPLARCVQIGLDLSSALSFAHRQR
                     IVHRDVKP"
BASE COUNT         2194 a         4109 c         4308 g         2206 t
ORIGIN      
        1 ggcgcgtcgg tggtcgtgcg cggtagctcg acggtgccgg cggtcagctc gagcgcgctg
       61 gtcgccatct ggaagatgaa ggcgacgagc aagatcgtca tcatgtccat catcggcatg
      121 atgttgaggt ggcggatcga ctcgccctcg ggcacgcggc caacggcctt gcgaatgagt
      181 gcgcgcgcct cgcgctgcgt aaagctcatt cgaacccact cgagaacagg atgtcgctga
      241 acagcgccat cgtcttcggg tcataggttg cgcgcgtcgt gtcgtagagg cgcttgtcgg
      301 gcgagatcgg gtccttcgcc ttcttcagct cgggatcctc ggtgggcagg gcgcagccgt
      361 cggtgtcctt gccgaactcg ggcatcttgc agcgcatcgc gctcatcacc gacgcgaccg
      421 tgttgtacgg aatcgcgcca tcggccatga ggatgatctg gtacgtgtcg gccttgcgct
      481 gcttgcctgc gtagcggcgg ttcgcgatct cgaacagcga cgccgacagc ccgcggtagt
      541 cgaacactgg cagcggtact tccttcgctg ggctcggctc gcacttctgc gtcgtgttgc
      601 gacagaagtt gctctcgcac atgtaattgc cgtcgcactg ctcgccgtcg cgaccactgc
      661 gggggaacgt cgcgtgcggc gcagcgagcg tgccttcgag gcccgagatc gaccacagga
      721 acatgcggtc gcgcgtgatc gagacgacca gcttcagtgg ctgctcgtcg ggattctggt
      781 tcgggttcgt ctgcgtcgtg gtcgcaggcg gcgctttgtc cgggagcgac gtatcgatct
      841 gcgtgaagat gatgccggct gaaatcgaga acagcaggaa cagcatcagg ttcgtgacca
      901 tgtcgagata ggggatcagg ttgagctcgc cgctctcgat ctcctcttgc tcgatctgat
      961 cttcgcgccg ccgaaccgca tggcgggtct tcgagcgaac gcgttgtgcc gagagatttc
     1021 ccacgtggcg ggcccttggc tccggttact ccacgtcctc agtcggggcc gctggcgcgc
     1081 ggctggccgc agccgccgtt ccgccgtcgg cgagcaggtt ctcgaaccgc aggctgaacg
     1141 cctcgaggtc cgcgaccacc ttcttcatgg ctgccgagag cagcaggtgg ccgaccatac
     1201 agatgagcgc gatcgagaga ccgaacgcgg tgttgtacat agcttcggaa ataccggtcg
     1261 acagccgctc gcgctgctcg gcggggttcg cctcaccgat cgcaccgaac gtacggatga
     1321 ggccggtgat ggtgccgagc agaccgacca gcgtgcagat gttcgcgagc gaccacagcg
     1381 cgccgatacg cgtcttcact tcgggcagcg tgtccgtcat cgtctcttcc atcgcctgcg
     1441 cgacggcagc ttcaccacga tgcagacggt tcaggcccgc cttggcgacg cgcgcgacgg
     1501 gcgcggtcgt ggcatcacac agcttctttg cgcgatcgac gttgttcgcc gacagcagct
     1561 tgcggagctg ctcgaggaac gccttcgcgt tgagatggcc gcggcccagg aagtagatcg
     1621 cacgctcgac aatcaggccg ataacgacgg ccaagaagaa cgtgttgacg atgaaaaagg
     1681 gcccgccctt ttggaagaca tcgcttagcc agctcatcga tcgtgcctgc ctttaagcgc
     1741 gcggggagac ctccctacgc ttgccaaatg ttgtagcgag ccagctcgcg tcgcgtcaag
     1801 caaaccaagg gcgatttcgg cttgtgtacc gagaacttac tgccgagatc gaatgctcaa
     1861 ttattcatcg cgccggccgt gatccagcgg tcgatcgcgt cgatcttctg acaacagagc
     1921 gtcgagttgc tcattggcat gtagcccacg ttgcctggcg gctccgcaaa ccctgcggtg
     1981 ttcatctcta cgccatggat gatgaagaac agatagctcg acgctgggtc ccctgccacc
     2041 acgcgttggt gggcggggtc taggttcgag gcggtaccgg tccccgcgct gcccatcagg
     2101 gacgtatacg ggtcgtccgt cagatcgagc tttccggagg ccgtggagcc gctatggcag
     2161 gacgaaccga agcagttcgt catgaaaatc ttgttcttga tccaggtgaa gtcggagtgg
     2221 ctgtccgcct ctttacacac agcggccatc gacaccttgc acgagcgccc cgacgtattg
     2281 gcgtcgacgt cggcgtgatc gagatcgatg cggcacccgg agacgagcag ggcggcgagc
     2341 aacacagcac gcatgggcga ggaacctatc acgtggtttg gcggcgcgcg tgtgacagtg
     2401 ccctcacgac caccggcaac tcagcgtttc gttacaaaca gtcgagggag ttgcacttcg
     2461 cgggtcgcgg aacggtcggc gtcggggtca gatcatcgaa ggcacgcact ggaaggctcg
     2521 ccagaaaacg gtacggcagc gtaccgcgga cgaacggcat ccagaccgag ctgccatcgg
     2581 tgtgcgggct cgccggatcg ccgctccacg gttcgacgcg cgcaaacgtc acattcggtg
     2641 gtggtgggaa gtcacggacc ttcgtgcgcg gatgcgcctt ggtcatgaag tcgacccaga
     2701 tcgggacagc gacgccgccg cccgtgatct tgtcgccgat cggtgtcgag tcgtcgcggc
     2761 cgatccacac gctgcacaga aggtcggtcg tgaacccggt aaaccacacg tccttgtagt
     2821 tcgcgctcgt gccggtcttg cccgcggtcg gccggccgat cgtcgtggcg tacttcgcgg
     2881 tgccacgcga cacgacgccc ttcatcatgt tgatgacgac gtaggcgacc tcgggcgaga
     2941 tgacctgcgg accgggcggc gggttgcgca gatcctcgac gaccgtgccg ccggtcgacg
     3001 tcaccagatc gaagaaccgc ggcgtgacgc ggcggccgcc gttcgcgatg ccggcgtagc
     3061 cggtcgcgat ctcgatcggc gtgatgtcgg gcgtgccgag gctgatcgag atgtggcgcg
     3121 gaatgtccga cgtgatgccg aacccgcgca ggatctcgat gatgcgatcg agaccgacct
     3181 gcgtcgcgat ctgcacgctg atcgtgttga gcgagtacgc gagcgcggtc atgagcgtga
     3241 cgttgcccat gtacttgttg tcgtagttcg ccggcatcca cgtgccggtg gcggtctgca
     3301 ccgcgaacgg gccgtcgtac atcgtgtcga ccggcgtctt gcccgcttcg atcgcggacg
     3361 agtaaatgaa cggtttgatc gacgagccga cctgacggtg cgcctgcgtc gcgcgatcga
     3421 actgcgacgc ggtccagtcg tagccgccga cgagcgcgac cacgcgacca gtgctcggct
     3481 ccatcgcgac gagcgagcct tgcagggcag ggcgctgcgc gatcgtggcg ccggtgccct
     3541 cggcgttcag ccgcaccgcg agcaggtcac cgagctggat gcgcttgtcg tagtcgatcg
     3601 tgttgtcctt cttcttcgga tgccaggcga ggacgtcttt cgcgtccttg tcgtcgagtg
     3661 gcagccgctt cggaccgaga tcgacgatca cgccgccttt cttcgtcagc tcgacgatca
     3721 tcgcgccgta gcgctgctcg ggcagcaact gatcggcgag cgcgctggtg tcgtcggtac
     3781 cgcccgacag cggatgtgcc gggccgccgg tccacgcgcc gcgattcttc gtcgccacgt
     3841 tgccaatcgg gccgcggaac ccgagccggc ggtcgagcga ctcgaggcct ttcttgagcg
     3901 cgctctcggc ggcggcctgc atcgtcatgt cgagcgtcga gtagaagcgc agcccgccct
     3961 tgaacagctc ggtgttgccg tagcgcttcg tcgcgagctt gcgcacgtgc tcgacgaagt
     4021 acggcgatgc aaggtgattg agatcgcctt cgtcgacgag cgcgatcggc tcggcgaggg
     4081 cctggttgta ctcggcatcc gagatgtact gatcctcgcg catgtggccg agcacgtagc
     4141 gctggcgttc gcgcgcgagc tgcatgttgc gatgcggtgc gtacttcgtc ggtgacgcca
     4201 cgaggccggc gagcatcgcg gcttctgcga tcgtgaggtc ctcgacctct ttgccgaagt
     4261 acgtctcggc cgcggcgccg acgccgtacg cgccgtgacc gagatagacg tggttgagat
     4321 agatcgagag gatctgtgcc ttcgtgagct cgcgctcgag gcgatacgcg aggatcacct
     4381 ccttcatctt gcgcttgtac ttctgggtct tcttgttggc ggcgagttgc tggggcgtga
     4441 agccgacctc cgagaggtcg ccttcctcgt cggcgagcag cgtctgcttg atgatctgct
     4501 gcgtgatcgt cgagccgcct tgcttgatgc cggtcgagcg gaagttcttc caggcggcac
     4561 gtacgatgcc ggcaaggtcg aaacccggat gcttgtagaa gcggcgatcc tcggcagaca
     4621 agaacgccgc cggtacgtgc ggcggcatgc gctcgagctg gatgatgcgg cggttctcga
     4681 tcgagaacgc gccgatttct tcgccgtccg acgacagcac gatcgacttt cgatacggct
     4741 ggtactcgag cagcttcgtg agatcctggg gcaggtcctc gttgatcgtc ttgatcgcga
     4801 agtagagcgt agccgccgct agcgacacgc cgtagaggcc gagcaggagc acgatgcgga
     4861 ggacggacgc ggtccccagg cccttgcgcc atcgccgcca caggctgcgc ccgacaaaca
     4921 tcgtgatgag gcccgcgacg atcgggagca cgtcggtgaa cgagatgtgg ccatccgcga
     4981 acaccgaaac cgccgtgttc actgcgccgg cgacggtcac gatgaatcca agcgcgatgt
     5041 agatccaacc gcggcggata tccgcgcggc ggcgctgttc acgcgagcct tgcttcgggc
     5101 gtattggggc cgccacgggt acaatcagtc tatgaggtct cggctgccgc cgcacgtctt
     5161 cgccgtcctg gcgatgtgcg tcgcgtccgc gggggcgcag ggaccggcgc ctacgcaggc
     5221 gaccgcaccg gctcccgaca tcgacgagcc cccggtggcc gcaaaagcgg cgccgagcga
     5281 tgcgaaactt tcggcgtcgg cggtgccgga ctacacggcg gggccgatca agatcgcggt
     5341 caccccgttc gagaaccacg tgccgaacgg taagtcgctc gaatgggtgg tcgccgaggc
     5401 gccgttcgag atcgccgaga aggccgagaa tatcctcgac ctcgaagcgg tgaatgcgcc
     5461 gctctacgta ccgggcgagc gcgtgccggc agagtccgac accgtcgcgg acttcgcgaa
     5521 gaagatgggc gccaagctcg tcatcaccgg ttggtacgat cgactcggcg agcagctgcg
     5581 cctcgtcgtg ctcgtgtggc aggccgacgg agcgaacgcg aaggtcgtcg gtgaagcgca
     5641 gcgcatcggc gcggtcggca gctatcacaa gacgctcggt gacgcgatgg ccgaggcgtt
     5701 cggcgaagcc ggcatcccgg tcgacaacgc gcgtaccgaa cgcctgagtc gcacgctgtc
     5761 gaacgacatc tatccggtct tcatgatggg ccgcggcctc ggcaacttca gcggtgcgct
     5821 cgccgcgatg agctcggtgt tcggcacggg cgctggctcg ggatcggctg cgaccggacc
     5881 cgatctgaaa gccgccgaac acgatctcga gcgcgcggtg tttctcgatc cgaaattgtt
     5941 cgaggcgcag cgcctgctcg gcgagctcta catgcagacc gcggccggcg atccaaagct
     6001 gatcaacaag gcgaccggca agttcaatta cgccgcggac ctcgcgcccg acgatgcgcc
     6061 gagcctgcgt gctgcggcgt tcgcgatggc gcgcgcgcag aagtgggaag tcgcgctcga
     6121 tctgttccgc aagctggtga cgcgccggcc gtgggatctc gaggcgcgct atcagctcgg
     6181 cgccgcgctg tggcagaccg gcaacgcaaa ggcggccgag cgccagctcg agcaagtgac
     6241 tgcgaaacag cccgaccatc ttccggcgcg tcgcgtgctc gtgctgattc actcggcgca
     6301 caacgacacg ccgcggctga tccgcgagct cgaagcgatc gcgcagcgcg cgcctgacga
     6361 tctcgacgtc aaggccgacc tcgcgaccgc gtacggcgcg atgaagcaat ggccgaagat
     6421 gatcggcgcg ctcgaggaaa tcgagaagca gcgcccgaac gacctcgcgc tgctcgtgcg
     6481 gctcggcgac gcgtaccgca aacacggcac gctcgacaaa gcgctggccg cgtaccagcg
     6541 cgccgggcac atcgatcgag atgtcagctt gcccgggttc gcggcggccc aggcgctgtt
     6601 cgatgcgaat cggctgcccg aagcgatcaa agcgtaccag ctactgcaga acggtagcga
     6661 tcgcgcagcg gccgagcacg cactcggcac gatcgcttat ctgcaaggcc gcgctgacga
     6721 agccgcctgg tacatgcggc aatccgcgcg cagtgcgccg cgcaatctca cgatgcgacg
     6781 cgccgttatc gcggccgagc tgctgcgcaa ggacgcgccg gctgcacgcg tgcagctcga
     6841 ctacgcgctc gcgacgtggc cgcacgacgg ctttctgcac tacttctcgg gcgtcgcgta
     6901 tcacctcgct ggcgacgaca cgcaggcgcg caccgagctg accaccgcgc tcgccgaaac
     6961 gccgggcttg ccggcggcga ccgctgcact cgctgcgctg ggtgcgcgta gcccgctcgc
     7021 cgtcgactac aagccggagc tcgtgcggcc gtggggtgac ggcgacgcac tgcaagcggc
     7081 catcgagtcg tacgcggcga ccagcacggc gatggcgcgc gtgcgtgccg actaccagaa
     7141 ccaggtcctc gcgctgctcg gtgcactcgg gcagggcccg aagacgaccg tgaaggcagg
     7201 ctcggtgaag acgtgcccgg tcacccaggt tgccaagccg tgggagacgg cgcagaagtc
     7261 gctgcagaaa tacgcgcagc tcggcgccga gctcgaaacg acctaccgct tcatcgcgcg
     7321 gcacgacgag gtcggcatga ccgcgagcct gttgccgaac gcacgcacgc aggtcgccgg
     7381 cgcgaagaag agctatcgca ccgcactcgc cgacatgggc gagctacgct cggagtggac
     7441 gcgtggtctc acccccgagc tgcgcgtcgt cggctgcagc gacaagctgc tgcaagcggc
     7501 gctcaaggat ccgatgcggt atcgcgtcgt gcaggaagac aagccggaga tcacgggggc
     7561 gcaccaagcg ccgcgcccca aaccgcgcgt gacgttcttc gtcgataaca cgcgctgtgc
     7621 ggacaccgtc gacgtgtgga tcgacggcga gcacatcggt caggtcgcgc cgggccggcg
     7681 cagcgctctc gtcaccgatg gtggcgcacg cacgctgtgc ctgctgttgc ccggcggtgc
     7741 gcaatgcggc gatcgcggca ccatgcgcca ggcctacctg cacgacggct ggagcacgac
     7801 gctctactgc ccgaagtaga gatccagcgc ccacgtaagc ctaggcgtaa gcgacatccg
     7861 agcagccgcc gtactctgcg cagcgcgcgc acaaagctaa gcggttgccg cttacgccta
     7921 cgcttacgaa gactcggatt acggcgccgc gaacgtgccg taggtcggcg cgcccgttcg
     7981 ctgatacgtc gcgatcacga tgccgttctg cgtcgtgcgc gagctcgcga gcttgaatgc
     8041 ggtcggcgtc gtaccggtct cgaacagccg cttgccgctg ccgacagcga ccgggaacac
     8101 gagcgtgttg atctcgtcga cgtagccgcc gttgatcagc gactggcaca ggccgtggct
     8161 gccgtggacc tggagctcgc ccgtatagcg cgccttgatc gcgccgatct cgtcggcaag
     8221 gttcctcacg atgaccgagt tgttccacgt agtgcgatcg agcgtgcgcg acacgacgtg
     8281 cttgggcagg ttgttcagct tgctggcgac cggatcgttc ggatccgtca tcttcggcca
     8341 gtagctttcg aagatgcgat acgtgccgcg tccgagcagg aatgcatcga cgcgtgaaaa
     8401 cacgtcggtc atgaactcgc cgaagacttg gtcgacgtgc ggcacgagcc agccgccgtt
     8461 ttgaaagccg ccgctggtgt cttcttcgcg gccgccgggc gcctgcatga cgccgtccag
     8521 agtgatgaag gtggtgatgg tgagctttgc catgttcgtt ctccttgtct gggcgacgaa
     8581 cgacccgcgg cagaatcgac accggcgtac ggttttctgc gaagcccgcg aacggactag
     8641 gaatgctacg gttcggttcc gtgcacctgc cgcatctggc attcggctcg ccgcgtctac
     8701 ttcccaagcc cgagaaggtt ccggggcgcg tggtcgtgct cgacatcgcg ttcgccgcga
     8761 cggtcggtac gagcgtgtcg ttcgtgctca cgacgaagcc gttcatcgat gggctcggcg
     8821 accggctcgc cgcgtgggtc gaccatcacg atcacgaacg tcacgccgac ttcgcgagcg
     8881 atccgcggtt cgtgctcgcg acgaaggcac agcatggcgc gtgccccgag atggtgacgc
     8941 cggatctcgt acggcaaacc ggaccgatcg attcgatcct cacgcacgtc gatctcgacg
     9001 gtctctatgc ggccgcgaaa tggatcatgg ccggcaagga gccatacgca ggagctgacg
     9061 atgacgcccg tgcggtcgat acgcgggtcg gcacgccggg gccgatcgcg aaccggatcg
     9121 atcgcgcact gcgcgcgaag tttcgcgacg accagctcaa gcgcgcggtc gttcactacc
     9181 tcgtcggcgg catgaagccg gggccgcacg acgacgtgat caccgaagca gccgcggagt
     9241 tcgatcgccg cgatgcgcgc acgcaggagc tcgcgcgccg gttcacgatt cgcggccggg
     9301 tcgcggtggt cgacaccgcc ggagcggccg gccagttcga caagaccgac ttgctactgg
     9361 ccggacaggc gaaggcgcag gtctcgatcg tgcgcgactc cgggatgctg acgattgcag
     9421 ctccgttcga ctccggttgg gacttcgtca cgatgttcga gctcggcggc gggatgccga
     9481 cgcgggtgac gattccggag cagaaactcg acgacgtgat tggaaaaatc aacgacgcgc
     9541 ctgcacctac gccgcgcgtc ggctcgacac cggacgactg atcgtcggag agtcttgccg
     9601 gatgtagaga cgtccgctat aacggcggtg gcgttcacta tcacggagat acagtcatgg
     9661 cacttcagct tggcgacgag gcaccgaact tcactgcaga gaccaccgag ggtcaggtca
     9721 acttccacga atggatcggt aacagctggg cggttctgtt ctcgcatccg aaagacttca
     9781 cgccggtgtg cacgaccgag ctcggtcggg tgtcggcgct caagggcgag ttcgataagc
     9841 gcaatgtcaa ggtgatcggc ctctcggtcg atccgctcga cgatcacaag cgttgggtcg
     9901 gcgacatcaa ggagacccag ggccacgcgc tcaacttccc gctgatcgcg gatccggatc
     9961 gcaaggtcgc gaacctctac ggcatgattc atccgaacgc gaacgacacg ctcaccgtgc
    10021 gctcggtgtt cgtgatcggc ccggacaaga agatcaagct cacgctcacg taccccgcga
    10081 gcaccggccg caacttcgac gagctcttgc gcgtgatcga ctcgctgcag ctgacggcga
    10141 agcacaaggt cgcgacgccg gtcgactgga agcagggcca ggacgtgatc atcgtgccgt
    10201 cggtttccga cgctgatgcg aagaacatgt tcccgagcgg ctggaagacc ctcaagccat
    10261 atctgcgcgt cgtcccgcaa ccgaagtcgt agtgacacgc gctgcgctcg tcgcggtaca
    10321 gctcgaaatt ggcgccgatg tgctggtcac gcccgaggcg taccggttgc acatcgaggc
    10381 cgagggcgag cgtgcgctcg ctgaaggcgg tggcgagaaa gccgacgcgc ggctcgtcgt
    10441 ctatccggag atcgcgggac acctcgcgct ctacgcgctc gcgcctcacg tcgcacggcg
    10501 cgcgaagaca ctcggcggtg cgctcgcagc agcagcggcg cgccggcctc tcgatgtcat
    10561 tcgtggcgtc gcgacgacgc gcttgctcga tgcacgtcac gcggtgctcg ctgcgctcgc
    10621 gcccgatggc gagcgctggt ggaagagcgt gttcggtccg ctcgcgaggc gactcgatgc
    10681 gtacatcgtc gcgggctcgt atctgcggct cggctccgac ggtgcgctca cgaatgcgtc
    10741 gctgttgttc gcgcccgacg gtcgactgct cgcgacgacc gacaaggtga acctcgtgcc
    10801 cggcatggag gatggcgcac gcggtgcgct cgcactcgcg cgaggcgacg cacggattcc
    10861 gatcgtcgaa acaccactcg gccgggtcgc gacgctcgtt tgctacgacg gatttcgcga
    10921 accgcacacg agcaccgagc ggttcgtcga gctcgggccg ctgctcgccg agcgcggcgg
    10981 cgtcgatatc gtcgcgaacc cggccgcgaa cccgtggccg tggaaagggc cgtggaagtt
    11041 caacgagccc ggcgaatcgc tggttcgcga agagcagtgg cgactcgaag gcctgaccgg
    11101 gtcgctgacg aagtcggcat tcgcgaagta cggcatcacc gcgcatctcg tcggcaaagt
    11161 gctcgacctc gcgttcgatg gcgcatcgga aatcgtggcc tgcgagggtg gggtgacgcg
    11221 ggtgctggcg cgtgccgagc accacgatcg aggtggtcac gtgacagccc tggtaacgtg
    11281 ctagacgtac gtcatgggtg aacgtcgtcc gctcgcggtt aggcagccag gcgcaccagg
    11341 tgtgcccgag gcaccgagtg cggcagacga ggtcgcggag ctgttgctcg aggcctacct
    11401 cgatccgcgc gcggccgaga tgcagcacat cggtagctac gagctaccga gcgatggcca
    11461 ggtcgagcgc gtgctcgaga tgtgccgcgc gctgctgttc cccgggtatg ccggtcccga
    11521 tatcgagcgt atggagaagg ggtcgctgcg cgagctcgtc ggcctgcgcc tggaggagct
    11581 gcgctacgcg cttcatcgtc aggtctatcg cgcgagtcac catcgcaagc agcagctcct
    11641 cggccgcaac gagctcgagt gcgcggaatg cacgcgcaac gccgatgcca tcaccgatcg
    11701 cttcatcgcc cggcttcccg agctgcgcgc gatgatccgc ctcgatctcc gggccgccta
    11761 cgagagcgat ccggcggcga cggggatcga cgaaatcctg ctgacctatc ccggcgccta
    11821 cgcggtcacc gtctatcgca tcgcgcacgc gctgctcaaa gaaggcgccg tgatcgtccc
    11881 gcgcatgatg accgagctcg cgcaccgccg gaccggtatc gacattcacc ccggtgccga
    11941 gatcggccat tcgttcttca tcgaccacgg tacgggcgtc gtgattggcg agaccacgct
    12001 gatcggcgac cgtgtccgca tctaccaagg tgtgacgcta ggcgcgctga ccgtgccgca
    12061 gggcgccgcg cggccggagc cgggtaggcg ccgtcatcca accatcgaag acgatgcggt
    12121 gatctacgcc aatgcgacac tccttggtgg ggacaccgtg atcggcaagg gtgcggtcat
    12181 cggcggcaac gcgttcatca ccgcgtcggt ggcgcccggt acgaagattt caggctcgag
    12241 ccgaacgcag aagtagcgct gcgggccttt tttcttgcag ccttcccgcc gtgttaagct
    12301 ggcgctgggt cgatgacgga cgacgtccaa gctcgcccgc gtaccaatac gcgtctcaaa
    12361 ggtgaggcac cgccagggct ggcgctctcc ggagaggaag ggcgcaagtc gatcggccgc
    12421 tacgaggtcg tcaagacgat cggcaagggc gcgatgggcg tggtctacaa ggcgcgcgac
    12481 ccgctgctcg atcgcatcgt cgcggtgaag acgatcatgt cgccgcagca gcaaggccgc
    12541 cgcgtgcggt cggcattcct cgagcgtttt caacgcgagg ccaaagccgc cgcgaagatg
    12601 tcgcatcccg cgatcgtcac gatcttcgac gtcggcgtcg acgaagaatc gggtgcgccg
    12661 ttcatggtgc tcgagtatct gcccggcgaa tcgctcgccg atcgcctcga tcgcgtgcgg
    12721 ctgccgctcg cgcgctgcgt gcagatcggt ctcgacctgt cgagcgcgct gtcgttcgcg
    12781 caccgccagc gcatcgtcca tcgcgacgtc aagccgg
//
LOCUS       DASUFX010000485         8722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1882886, whole genome shotgun sequence.
ACCESSION   DASUFX010000485 DASUFX010000000
VERSION     DASUFX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882886"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VFV99_19465"
     CDS             complement(<1..1129)
                     /locus_tag="VFV99_19465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX5061560.1"
                     /translation="MRHVVVGALLLANCQQSDKKNESSTSGSAQASSSAISTPAPQSS
                     AAESARAPQAPLPPLPDPLPGKRTDLTAVIGSATRAAIGDFNGDKKRDIAIVDGKQIR
                     ILDAAGKELASAPVTSGILVLTAADIDGDGKSELLAGWGQTREVRDGTARVTVHRLKG
                     KELVEQTIVEPATSRQEVVAIVPTDKRTLLLAYFDSQYMVTNAIAHEEASGWKVENGA
                     SIRMATTYARGDVDGDGKPDLVVGRVYGDDKGVDGDAFVLAPDGTRTKLPTTRGLRSL
                     AVVGSDIYFGDGWHQNYGQNAHGFLTHVRHDKDGFRSDLIEDTAGQYAIERILPATID
                     GKPALVTLGSHYVRVFARDGDRWRGLTIAGMARDIAVGDLDG"
     gene            1251..2453
                     /locus_tag="VFV99_19470"
     CDS             1251..2453
                     /locus_tag="VFV99_19470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX5061561.1"
                     /translation="MRRGAVVLAVVLTGCLGEVTDMGSPADEPAPNDEPLAGCAMACH
                     GADSSNAPPKSISGATDTTSVGVGAHQSHMLAAPTWHRKVDCADCHVVPTDLGSPGHI
                     DGDNKAELTFGMIAGAGSTWNGTTCTTHCHGETAWGGNKNEPQWTQVDGTQSTCGSCH
                     GAPPPAPHPTDTNCAACHPTMEENSLVFRDPASHINGIVDLVDSATTGGCTTCHGSTT
                     AAPPKDLAGNTARTSKGVGAHEQHLATSTWHRDVACSSCHVVPQTLNSPGHRDGDNIA
                     EIIFDTLNPTGVYTVATTTCSNQYCHGNGRGSNGTISWLTTGPLACNACHATNGQGMS
                     GDHSKHINGENMQCSGCHRDVVQGSTTIINALLHVDGAKQVKMAQGTYNPTTRQCSNT
                     GCHGTKTW"
     gene            complement(2454..3227)
                     /locus_tag="VFV99_19475"
     CDS             complement(2454..3227)
                     /locus_tag="VFV99_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061562.1"
                     /translation="MKLTIVLPFVVSVFAACFSMPQPAPAPNPGQIEAQQKAAFAQSM
                     GYPTRKTVQDLEAVIAQQTKDFKRTGRKDEARLEAFAPFEIDGVKGTCYTIVMRLGDG
                     ATWGPSADYLKFDFHSPTGPGSGGPGVTGPGAVVSVGCAEASGPIALTMAPMVGKDPI
                     GQGPVELELWSHKLTAQEAAHLEADKQRQIQEQKDFAAREEAKRQQRANTGCAKCDGR
                     YQGCIGAGRSQSVCRDEYGSCAFREVGADYLSACPSPSY"
     gene            complement(3277..4503)
                     /locus_tag="VFV99_19480"
     CDS             complement(3277..4503)
                     /locus_tag="VFV99_19480"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5061563.1"
                     /translation="MFAKQTAWSASLLALCGAAAVARAEDEVSAQSEGEVHPPGETQP
                     TPVETEPGPPDPSDSAPPDPRKPAPPDPPPPPKRHTGTFTIGAGFSTDEGPIAVASIA
                     QDDLFHTGNHLSLHAQLSARRQLFLERFVDPDVLGSRLSFEADLYSDTHQLPGFTRKA
                     AGGSLTLSHPIGEHTRAFVGYRLEDVSTTDNVRLFARTIDPLPPLTGGILSAVRAGVV
                     YSTLDRADAPLRGTQIGGSIEVADPAFGSDLQFTKTDGWIQHHAPIGPLTLHVAGSFS
                     TIAGPGGLFSTIGGPGGPPRSERLFLGSSNEIRGYRPDSFGPVDSLGTPVGGVAKVLG
                     SVELEVPLVRRIGLSAIGFADTGGLFDRSGQGQTGTSVGFGLLWRSPIGALRFNWAIP
                     LDGSRPGFVFGIGSSF"
     gene            complement(4543..5445)
                     /locus_tag="VFV99_19485"
     CDS             complement(4543..5445)
                     /locus_tag="VFV99_19485"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5061564.1"
                     /translation="MPKHASPVEKYIEQYPRPPFPPKKLEEPGLEAELEPRPRYQAPE
                     YHAANKLAGKRALITGGDSGIGRAVAVLFAREGADIAINYLDVEQRDAEETKRAVEAE
                     GRRCVLIAGDLTDEKFCRELVERAVNELGGIDILVSNAAHQTRKRLEDLELAEFDRTF
                     KTNVYAYYLLAKYAAPHMPPGSAIIATSSSTGFRGSEELPDYSATKGAINQFTKVLAK
                     QLVEKGIRVNAIAPGPVWTPLNPADAGLPPDKLAEFGSKTPLERPAQPEEVAPAYVFL
                     ASEADSSFITGIILPVLGGETIGA"
     gene            5541..5726
                     /locus_tag="VFV99_19490"
     CDS             5541..5726
                     /locus_tag="VFV99_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061565.1"
                     /translation="MTKLFALGGVGAIGAGVFAAVRRARRNAAMKRAVSDAFDYGDLD
                     NPVVVSEEFVIVGAAPP"
     gene            complement(5727..6359)
                     /locus_tag="VFV99_19495"
     CDS             complement(5727..6359)
                     /locus_tag="VFV99_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061566.1"
                     /translation="MRAVSGAAFGILAFLISGADAAADGEATPMIGGSVVWSPSAEQP
                     SGMIGLAVEAAWWSGRVGVAVEGSGRSYVDNDQPRAFVLGSSVRLRLFDGLWRSWVDA
                     RDVEVGIELQGILERWWYQRNEPELDPARYGVGLALRVRGGADDGSLRISESRFFIRV
                     LAAPTVTDHAVARSMTPPAEEPRELMLLIGIGAAFGSSDPDYIERFRWHE"
     gene            complement(6458..6961)
                     /locus_tag="VFV99_19500"
     CDS             complement(6458..6961)
                     /locus_tag="VFV99_19500"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX5061567.1"
                     /translation="MSIYPFILEMKKLLRSLDGCIDKAVAHASAKKFDPNVLVQARLA
                     PDQFAFVRQVQATCDQAKFAASRLAGKEPPSHPDTEQTMDELKKRIATVVSYLDTFSE
                     KDFAGVGDRTVTTPRWEGKSMTAINYFVEHSQPNFYFHLTTAYAILRHNGVDIGKRDY
                     IGQLTWR"
     gene            complement(6998..7498)
                     /locus_tag="VFV99_19505"
     CDS             complement(6998..7498)
                     /locus_tag="VFV99_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061568.1"
                     /translation="MRAPALWGIAGVVALLGDALYRLTPQAIGLFEQSLDAIEISVLV
                     VWLAFNAYSEGYRAFHRNFSPRVVARARALDAAPRPLFVILAPLYCMGLVHATRRRLV
                     VSWCITVGIVGIVILVRQLAQPWRGIVDAGVVVGLAWGVASIVYYVIRAFRGHAMPVA
                     PDLPVR"
     gene            complement(7499..>8722)
                     /locus_tag="VFV99_19510"
     CDS             complement(7499..>8722)
                     /locus_tag="VFV99_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061569.1"
                     /translation="DRRAARDRRGGRRDRRRDLGGYVLMRVWWLLLALGCGKELNPEF
                     CAAHPDDSRCIAADAKHDGAGGKFDIAHLSPAVEDMLTSTSDVRILSMTMIDTSTGTI
                     SPGASGAFVLSTIGQENGPDVMVMQAASFTLDAHITVRGDKPLIIVATNDISVTMAID
                     ASANMMMPGAGGGQQTSGMGVGGNGMAGSSNSDAGGGGGGFGTAGGAGGAVDNREGGL
                     AGVSYGDATMLVGGSGGGRGAGPGSCLPSAGAGGGAIQLSAGGTLTIDGSINVGGGGG
                     SGGPGCGSTVNDGAAAGGGGSGGMIFVQARTFNGAGMLGANGGGGGGAASAGGTSMGM
                     PGENASLTGGGTGGAGGVNGGAGGTGASTGDGENGGDNVNANENGGGGGGGAGRIFYK
                     GPQPGYTANPPATSA"
BASE COUNT         1523 a         2989 c         2792 g         1418 t
ORIGIN      
        1 cgccgtcgag atcgccgacc gcgatatcac gagccatccc tgcgatcgtc aggccgcgcc
       61 aacggtcgcc atctcgcgcg aacacgcgga cgtagtgact accgagcgtg acgagtgctg
      121 gcttgccgtc gatcgtggcc ggcaggatgc gctcgatggc atattgcccg gcggtatcct
      181 cgatcagatc gctacgaaag ccgtccttgt cgtgacggac gtgggtcaga aagccatgcg
      241 cgttctgccc gtagttctga tgccagccgt cgccgaaata gatgtcgctg ccgaccaccg
      301 cgagcgagcg cagaccgcgc gtcgtcggca acttcgtacg cgtgccatcg ggcgcgagca
      361 cgaacgcatc gccgtcgacg cctttgtcat cgccatagac gcggccaacg acgaggtcgg
      421 gcttgccgtc accatcgaca tcgccgcgcg cgtaggtcgt cgccatccga atcgacgcgc
      481 cattctcgac cttccagcca ctcgcttcct cgtgcgcgat cgcgttcgtc accatgtatt
      541 gcgaatcgaa gtacgcgagc agcaatgtgc gcttgtcggt cggcacgatc gccacgacct
      601 cctggcgtga cgtcgccggc tcgacgatcg tctgctcgac gagctctttg cccttgagcc
      661 gatgcacggt gacgcgggcc gtgccatcgc gaacctcccg ggtctgtccc cagccggcga
      721 gtagctcgct cttgccgtcg ccatcgatgt ccgccgcggt gagcacgagg atgccggacg
      781 tgaccggcgc agacgcgagc tctttcccgg ccgcgtcgag gatccggatc tgcttaccgt
      841 cgacgatcgc gatgtcgcgt ttcttgtcgc cgttgaagtc gccgatcgct gcgcgcgtcg
      901 cagagccgat cacggccgtc agatcggtgc gcttgcccgg caacggatcc ggaagcggcg
      961 gcagcggtgc ctgtggcgct cgagcggatt ctgcagccga tgattgcggc gcaggtgtcg
     1021 agatcgcgct ggacgaagct tgcgcagatc cggaggtcga tgactcgttc ttcttgtcgc
     1081 tctgctgaca gttcgcgagc aacagtgcgc caaccaccac gtgtctcatt gcgagcgcat
     1141 cgtagcgcgt gctgttaccg gcgcgcccat ggggcgtgtc cgcgaattgc gcatcatcgc
     1201 gtccctcaat tgcgcaacac gactttgaaa gcgctgtgta gggtggtctc atgcgaaggg
     1261 gagccgttgt actcgcggtc gttctaaccg gatgcctcgg cgaggtgact gacatgggca
     1321 gtccggccga tgagccggcg cccaacgacg agccgctcgc cggctgcgcg atggcgtgtc
     1381 acggcgccga ttcgagcaat gcaccaccga agagcatctc cggcgcgacc gacacgacgt
     1441 cggttggtgt cggcgcgcac cagtcgcaca tgctcgcggc cccgacgtgg catcgcaaag
     1501 tcgactgcgc cgactgtcac gtcgtgccga ccgaccttgg ctcgccgggt cacatcgacg
     1561 gtgacaacaa ggccgagctg acgtttggca tgatcgcggg tgcgggatcg acctggaacg
     1621 gcacgacctg cacgacgcac tgccacggcg agaccgcatg gggcggcaac aagaacgaac
     1681 cgcagtggac gcaggtcgac ggcacgcaat cgacatgcgg cagctgccat ggcgcaccac
     1741 cgccggcacc gcatccgacc gacacgaact gcgcggcctg ccatccgacg atggaggaga
     1801 actcgctcgt attccgcgac ccggcgagcc acatcaacgg catcgtcgat ctcgtcgact
     1861 ccgctacgac cggcggctgc acgacgtgcc acggctcgac gacggcggcg ccgccgaagg
     1921 atctcgccgg caacaccgcg cgcacgtcga agggcgtcgg cgcgcacgag cagcacctcg
     1981 ccacgtcgac ctggcatcgc gacgtcgcgt gctcgagctg tcacgtcgtg ccgcagacgc
     2041 tgaattcgcc cggccaccgc gacggcgaca acatcgccga gatcatcttc gacacgctga
     2101 acccgactgg cgtctacacc gtggcaacca ccacgtgctc gaaccagtac tgtcatggca
     2161 acggtcgcgg cagcaacggc acgatctcgt ggctcacgac cggtccgctc gcctgcaatg
     2221 cgtgccacgc cacgaacggc caaggtatgt ccggtgatca ctcgaagcac atcaacggcg
     2281 agaacatgca gtgcagtggt tgccaccgcg acgtcgtgca aggcagcacg acgatcatca
     2341 acgcgctact tcacgtcgac ggtgccaagc aggtcaagat ggcccagggc acgtacaatc
     2401 cgacgacgag gcagtgctcg aacaccggct gtcacggcac caagacctgg tagctagtag
     2461 ctcgggctcg ggcacgcgga caggtaatcg gcgccgactt cgcggaacgc acagctgccg
     2521 tactcgtcgc ggcacaccga ctgcgagcgg cccgcgccga tgcagccttg atagcggccg
     2581 tcgcacttcg cacagccggt gttcgcgcgc tgttggcgct tcgcttcctc acgcgctgcg
     2641 aagtctttct gctcctggat ctgacgctgc ttgtccgctt cgaggtgcgc tgcttcctgg
     2701 gcggtgagct tgtggctcca tagctcgagc tcgaccggac cttggccgat cggatccttg
     2761 ccgaccatcg gcgccatcgt gagcgcgatc ggcccgctcg cttccgcgca accgaccgag
     2821 accacggcgc cgggccccgt cacgccaggg ccgcccgagc cgggaccggt cggcgagtgg
     2881 aagtcgaact tcagataatc cgcgctcggg ccccacgtcg cgccgtcgcc gagccgcatc
     2941 acgatcgtgt agcaggtgcc tttgacgcca tcgatctcga acggcgcgaa cgcctcgagg
     3001 cgtgcttcgt ccttgcggcc ggtgcgcttg aagtccttgg tctgttgcgc gatgacggcc
     3061 tcgagatcct gcacggtctt ccgcgtcgga tagcccatgc tctgggcgaa cgcggctttc
     3121 tgctgcgcct cgatctgccc aggattgggc gcgggcgcgg gctgcggcat cgagaaacag
     3181 gcggcaaaca cagagacgac gaacgggagg acgatcgtga gcttcacgca atcacgagcc
     3241 acgagacggc gcgcggtcgc atgaaaagtg cgcgggtcag aaactggagc cgattccgaa
     3301 gacaaagccc ggtcgactgc catcgagcgg gatggcccag ttgaaccgga gcgcgccgat
     3361 cggcgagcgc cacaacaggc cgaagccgac cgacgttccg gtctggccct gcccgcttcg
     3421 atcgaacagg ccgccggtat ccgcaaagcc gatcgcggac agcccgatgc ggcgaacgag
     3481 tggcacctcg agctcgacgc tgccgaggac cttggcgacg ccaccgactg gcgtaccgag
     3541 tgaatccaca ggcccgaacg agtcaggtcg atagccgcgg atctcgttcg agctaccgag
     3601 gaacagccgc tcgctccgtg gtggtccacc ggggccaccg atcgtcgaga acaggccgcc
     3661 gggcccggcg atcgtcgaga acgaaccggc cacgtgcagt gtcagcggac cgatcggtgc
     3721 gtgatgctgg atccagccat cggttttcgt gaactgcagg tcagagccaa aggccggatc
     3781 cgcgacctcg atcgagccac cgatctgcgt gccgcgcagt ggcgcgtcgg cgcggtcgag
     3841 cgtgctgtac acgacgcccg cgcgtacggc cgacaggatg ccacccgtta gcggcggcaa
     3901 cggatcgatc gtgcgcgcga acaggcgcac gttgtccgtc gtactgacgt cttcgaggcg
     3961 atagccgacg aaggcgcgcg tgtgctcgcc gatcggatgc gacagcgtga gcgagccgcc
     4021 ggccgccttg cgcgtgaagc ccggcagctg gtgcgtgtcg ctgtacaggt ccgcctcgaa
     4081 cgacagccgc gagccaagca cgtcaggatc gacgaaccgc tcgaggaaga gctgccgtcg
     4141 cgccgagagt tgcgcgtgga gcgacagatg attgccggtg tgaaacagat cgtcttgcgc
     4201 gatcgatgca acggcgatcg ggccctcatc cgtcgagaaa ccagcaccga tcgtgaacgt
     4261 tccggtgtgc cgctttggag gcggtggcgg atcgggcggc gcgggtttgc gcggatccgg
     4321 cggcgcggag tcgctgggat caggcgggcc cggctcggtc tcgacgggcg tcggctgcgt
     4381 ctcaccgggc ggatgcacct cgccctcgct ctgcgcggac acctcgtcct cggcgcgcgc
     4441 aactgcagcg gcaccacaca aagctaggag cgaggcagac catgcagtct gcttcgcgaa
     4501 catttcgcag actacgacag cgcgagatgc gtggacggtt gcctatgcgc cgatcgtctc
     4561 gccacccagc acgggcagga tgatgccggt gatgaagctc gagtcggcct ccgacgcgag
     4621 gaagacatag gccggcgcga cctcttccgg ctgcgccggg cgctcgagtg gcgtcttgct
     4681 accgaactcc gccagcttgt cgggaggtag acccgcatcg gcgggattca gcggcgtcca
     4741 caccggtcct ggcgcgatcg cgttgacgcg gatgcccttc tcgacgagct gcttcgcgag
     4801 caccttcgtg aattgattga tcgcgccctt cgttgccgag tagtcgggca gctcttcact
     4861 gccgcgaaag cccgtgctcg agctcgtcgc gatgatcgcc gagccaggcg gcatgtgcgg
     4921 cgccgcgtac ttcgcgagca agtagtacgc gtacacgttc gtcttgaacg tgcgatcgaa
     4981 ttcagcgagc tccaggtctt cgagccgttt gcgagtctga tgcgccgcgt tcgacaccag
     5041 gatgtcgatt ccgccgagct cgttgaccgc acgttcgacc agctcacgac agaatttctc
     5101 gtcggtgaga tcgcccgcaa tcaagacgca gcggcgcccc tccgcttcaa cggcacgctt
     5161 cgtctcttcc gcatcgcgtt gttcgacgtc gaggtaattg atcgcgatat ccgcgccctc
     5221 gcgcgcgaac agcacagcga ctgcgcgccc gatgccggaa tcaccaccgg taatcaatgc
     5281 gcgttttcct gcgagcttgt ttgccgcgtg atactcgggc gcttggtacc gcggtcgcgg
     5341 ttcgagctcc gcctcgagac cgggctcctc gagcttcttc ggcggaaacg gcggcctcgg
     5401 atactgctcg atgtacttct cgacaggcga cgcgtgcttc ggcatgcccg gaagcgctgc
     5461 aatcgcgcag ccacgcacat catcgaagct cgcagcgacg gcaaggcata cgcgttgctc
     5521 ggtatctggt catgaggacc atgacaaaac tattcgcgct cggcggcgtc ggtgcgatcg
     5581 gcgctggagt attcgcagcg gttcgcaggg cgcgtcgcaa tgcggcgatg aaacgggccg
     5641 tgagcgacgc gttcgactac ggcgatctcg acaatcccgt tgtcgttagc gaggaattcg
     5701 tcatcgtcgg cgcggcgccg ccctgatcac tcgtgccaac gaaaccgttc gatgtagtca
     5761 gggtcgctgc tgccgaacgc ggcaccgatc ccgatcagca gcatcagctc gcgcggctcc
     5821 tcggcaggcg gcgtcatcga acgcgccacg gcgtgatcgg taaccgttgg cgccgcgagc
     5881 acgcggatga agaagcgcga ctcggagatg cgtagcgagc catcgtcggc gccgccgcgc
     5941 acgcggagcg cgaggccgac gccgtagcgc gcaggatcga gctcgggctc gttgcgctga
     6001 taccaccagc gctcgaggat cccctggagc tcgatgccga cctcgacatc gcgcgcatcg
     6061 acccacgacc gccataggcc gtcgaacaag cggaggcgca cgctcgagcc gagcacgaac
     6121 gcacgcggtt gatcgttgtc gacatacgat cgtcccgagc cttcgacagc gactccgacg
     6181 cgaccgctcc accacgcggc ttcgaccgcg aggccgatca tccccgacgg ttgttcggcg
     6241 gatggcgacc acacgacaga gccgccgatc atcggtgttg cttcaccgtc ggccgcggcg
     6301 tcggcgccgc tgatcaagaa tgcaagaatt ccgaacgcag cgccgctaac tgccctcata
     6361 gtgatcgagc atgcggatcc tcgtcggagt gcacagtccg gatgttgtcg cctacctcaa
     6421 atctatcctg cgtagggcga cgtagcgcgc tcgcgcctca gcgccaggtg agctggccaa
     6481 tatagtcgcg cttgccgatg tcgacgccgt tgtgacgcaa gatcgcgtac gcggtcgtca
     6541 ggtggaagta gaagttcggc tgcgagtgct cgacgaagta attgatcgcc gtcatcgact
     6601 taccttccca gcgcggcgtc gtgaccgtac ggtccccgac ccccgcgaag tccttctcgg
     6661 agaacgtgtc gaggtagctg accacggtcg cgatccgctt cttcagctcg tccatggtct
     6721 gctcggtgtc gggatgcgac ggcggctctt tcccggcgag acgcgacgcc gcgaacttcg
     6781 cctgatcaca cgtcgcctgc acctgacgca cgaacgcgaa ctgatcgggc gcgaggcgcg
     6841 cttgtacgag cacgttcgga tcgaacttct tcgcgcttgc gtgcgcgacc gctttgtcga
     6901 tgcagccgtc gaggctgcgc agcagtttct tcatctcgag gatgaacggg taaatggaca
     6961 tgcccgtgcg taacacggcc ggcggcacgc gtgcgcctca ccggacaggc aggtcgggag
     7021 cgaccggcat cgcgtggccg cgaaatgcgc ggatgacgta gtagacgatc gacgcgacgc
     7081 cccacgcgag accgacgact acgcccgcat cgacgatgcc gcgccacggc tgcgcgagct
     7141 ggcgtacgag gatcacgatg ccgacgatgc cgacggtgat gcaccacgac acgacgagcc
     7201 tgcgcctcgt cgcgtgcacg agccccatgc agtagagcgg tgcgaggatc acgaagagcg
     7261 gccgcggcgc tgcgtcgagc gcgcgtgcac gggcgacgac gcgtggcgag aagttgcgat
     7321 ggaacgcgcg atagccctcg gagtacgcgt tgaacgcgag ccacacgacg agcaccgaga
     7381 tctcgatcgc atcgagtgac tgctcgaaca gcccgatggc ctgcggcgtc aaccgataca
     7441 gcgcgtcacc gagcagcgcg acgacaccgg cgatccccca cagcgctggc gcacgcactc
     7501 acgcactcgt cgcgggagga ttcgccgtgt agcccggctg aggtcccttg tagaagattc
     7561 gacctgcacc gccaccgccg ccaccgccgt tctcgttcgc gttgacgttg tcgccgccat
     7621 tctcgccgtc gccggtactc gcgccagtgc cgccggcacc gccgttgaca ccgccggcac
     7681 cgcccgtgcc gccgcccgtg agcgacgcgt tctctcccgg cattcccatc gaagtaccac
     7741 cggcgctcgc cgcacctccg ccaccaccgc cattcgcgcc gagcattccc gcgccattga
     7801 acgtgcgcgc ctgcacgaag atcatgccgc ccgagccacc gccgccggcc gccgcgccat
     7861 cgttcacggt cgagccgcaa ccgggtccgc cgctgccacc gccaccaccg acgttgatcg
     7921 agccgtcgat cgtgagcgtg ccgcctgcgg aaagctggat cgcgccgccg cccgcaccgg
     7981 ccgatggcag acagctaccc ggaccagcac cgcgtccacc gccagaacca ccgacgagca
     8041 tcgtcgcatc gccgtagctg acaccggcga gcccgccttc acggttgtcg accgcgccgc
     8101 ctgcaccgcc agctgttccg aagccaccgc cgccaccgcc cgcatccgag ttactcgagc
     8161 cggccatgcc attcccaccg acgcccatgc cggacgtctg ctggccaccg cctgcgcccg
     8221 gcatcatcat gttcgccgat gcatcgatcg ccatcgtgac cgagatgtcg ttcgtcgcga
     8281 cgatgatgag cggcttgtct ccgcgcacgg tgatatgcgc gtcgagcgtg aatgacgccg
     8341 cctgcatcac catcacgtcg gggccattct cctggccaat cgtcgagagc acgaacgcgc
     8401 ccgatgcgcc cggcgaaatc gtgccggtcg acgtatcgat catcgtcatc gacaagatgc
     8461 ggacgtccga cgtgctggtc agcatgtctt cgacggccgg cgacaagtgc gcgatgtcga
     8521 acttgccgcc tgccccgtcg tgcttggcgt cggcggcaat gcagcgcgag tcatcaggat
     8581 gcgcggcgca aaactcggga ttgagctcct tgccgcagcc gagcgcgagc aacagccacc
     8641 acacgcgcat cagaacgtac ccgcccagat cacgccgccg atcacgccga ccgccccgcc
     8701 gatcgcgagc ggcgcgccga tc
//
LOCUS       DASUFX010000486        17706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_217433,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000486 DASUFX010000000
VERSION     DASUFX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17706
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217433"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFV99_19515"
     CDS             <1..975
                     /locus_tag="VFV99_19515"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX5061570.1"
                     /translation="PKKAAAPRKKKVKAAAGSVGLTADEVASGSPPAEVTKLEKQIEA
                     ADGKVLAVYREPLGGHWVALAALPLDKVKPTPYQRELSKAHADRLAMVIPKVGRFLDP
                     VIAVPDAAGTFITPNGMHRLAALGQLGAKTVVALVMPEPEIAFRILALNTEKAHNLKD
                     KALEVIRMARAIAAEDDRSEEKMSFELELPQLVTIGVCYEKNPRFSGGAYNSVVGKCE
                     SWSTDPMSKSLKLREAHADKLLELDELVGQAVQKLKDAGWTSGYLKPIVVARINPLRF
                     VKQGKDEKSDFDKTIDKMIDGAKKFDASKVKAQDVVLAATVGGGEDTN"
     gene            complement(995..1492)
                     /locus_tag="VFV99_19520"
     CDS             complement(995..1492)
                     /locus_tag="VFV99_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061571.1"
                     /translation="MLKTTLVAAALLTASGVAHAGGQEGSIGVGAEFMITPITPFALG
                     GASLTYDAGAFHVGGFLGFEDGDGPDNTDFTIGGRFYYHVHSTAMSDFGIGGSLGIVS
                     LDAPMGADRQTGLFIDPGAQIRLFLAANVAISAQVGVSIGTMDASGVAITGQTIGGGI
                     HYYFF"
     gene            1541..2560
                     /locus_tag="VFV99_19525"
     CDS             1541..2560
                     /locus_tag="VFV99_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061572.1"
                     /translation="MISAELDLHDLDARHWRNAWRLLVPPRVLDKPRWALVIIDGTPD
                     NVVKVIIAGADARGSIDPASAPLPGVGEKALGAYAKQLGVGSVIVVDRHVVKQLSSEI
                     ESTLRFDQDGIAQGLVALKALKKHAGKGVWTEPPLLELLPTPAMDPIQRTFDLLVPDR
                     SALVAYVIEDDCQRVHTSIIAVKEDGDIVRAATHRAIADLVPETKLALAWTTQYKRVL
                     DAVEERFAKPSIALFIERGTLMKVLTGPSDQLAREYNAKNVIIDPVPAWLLGLLGGAA
                     VAAMAGRAGKALAAMLPTATRERASALVARAQDVMKESGAHPFALLGFDPIELWSRLK
                     HHYRP"
     gene            complement(2640..3251)
                     /locus_tag="VFV99_19530"
     CDS             complement(2640..3251)
                     /locus_tag="VFV99_19530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5061573.1"
                     /translation="MPRPDNFIHATAIVDGKQTHVGSDTKLWHFVHVMEGAWIGDRCS
                     IGQGCFIGKVRIGHGCRIQNHVSIYDGVTLEDDVFLGPCCVFTNVKHPRAHVQRKDEY
                     ARTLVCKGATVGANATIICGVTIGEYAMIGAGAVVTADVPPHALVIGTPGRRVGWVCN
                     CGETLPDDLKCKRCGERYAELDGLLEHIPGDPLTTTQETLRRD"
     gene            3325..4161
                     /locus_tag="VFV99_19535"
     CDS             3325..4161
                     /locus_tag="VFV99_19535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5061574.1"
                     /translation="MRGAGPTRAHAADGNELWHADVLELLGRLPDGSVDLVVTDPPYA
                     IAKESWDEFESLDAYVDWCDRWLAEVARVLAPHGSAYVCGFSEILADVKARSAKRFAN
                     CRWLIWYYRNKANLGKDWGRSHESILHLRNDDARIDVDAVRVPYNGHTTKYPARVQAV
                     NSQYGRGVRRDRWEPNPLGAKPRDVIEIPVICNGMSEKTPHATQKPEALIERLILASS
                     AKGQLVVDPFVGSGTTAVVAAKLGRKWLAGDADARYVGLARDRLQASGSRLQPEPRTK
                     RS"
     gene            complement(4251..6560)
                     /locus_tag="VFV99_19540"
     CDS             complement(4251..6560)
                     /locus_tag="VFV99_19540"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX5061575.1"
                     /translation="MKRNAILCGLLVAACGGSNKQAVTTSDLGPSKQSSETASAEQHE
                     AAAPRPADPQLDFRRQYSDPGGMWMPMQMTLPQHVDTFQKMGVKLNAEALSDPLSDPL
                     AAIVSLGGCTASFVSPDGLIVTNHHCVQGALNYNSTPEKNLIENGFLAKTRADEILAS
                     PSQRVMVVQAYKDITKEMRDGLEKIKDPIKRKEESEKRYKQQVAACEKDRPWLRCQVS
                     SFFRGSMYQLIEMLEIKDVRLVYVPQRAVGNYGGEIDNWAWPRHTGDWSFYRAYVGKD
                     GKPAEFSADNVPFHPQHWLKVTTAGLKPGDFVMVTGYPGSTSRLDTASEIHHDVEWHL
                     PFYIEYLQQKYNLMESHLGDKNATAIKATNQKQFIQNGLEKFQGQLDGIKKSDILQRK
                     DELDKKVKEWAAQPGNEIHKANIEKLEHILADEFRTARVDFERGATFGGSKFLSTALS
                     LTRWAEQRAKKDMDRKPGFQERDLPRSVAGQKSFVRTFDRTLDRESFRINLMRSLALP
                     EADRPWLATLLGVKKGTKIDEALIDKTLDAWYKDTKLEDEKVRIDLLTKGTMAQIKAS
                     KDPFVMAAQRIWSTVKADEKKEDARAGELVLVTPSYAEGLKAVLGGALAPDANGTLRV
                     TYGTVKSFKPESKAQEDWAFTTASQILAKNTGKAPFDAPANELAAIKAKKFGPYADLT
                     LGGELPVDFLSDLDITGGNSGSPTLNNKGELVGLAFDGNKEGVSSDVLFNGQTSRTIH
                     VDARYMIWMMDAVDGADHLVKEMGLTPSL"
     gene            complement(6678..8750)
                     /locus_tag="VFV99_19545"
     CDS             complement(6678..8750)
                     /locus_tag="VFV99_19545"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5061576.1"
                     /translation="MSAVDLSKLNAPQREAASHGGGPLLVLAGAGSGKTRVITFRIAH
                     LLAQGVPAHAICAVTFTNKAAEEMRERIAHLLGDKQLARELTIGTFHALGLQILRMER
                     KALGLPRGFAIYDQSDQLGAIREAMRHLHDGDRRYDAKAVQTRISLAKNAFIAPDEYE
                     GNPADDYDAMTVQVYPKYQETLRACAAFDFDDLIVEPVRLFQRDEDVRARWASRFQYV
                     MVDEFQDTNKAQLRMVQSLVEQHGNLVVVGDDDQSIYSWRGADPTNILQFDRVFPGAK
                     IVKLEQNYRSTKNVLAAANTVIANNKDRHGKTLWSQLGDGEKITHAVAQSAEDEAKWV
                     AREIRNIHKDGRRFSDIAILYRSNLQSKVLEEELRAAEVPYVMYGGQQFFERKEVKDV
                     IAYLRVALNPRDELALRRVINYPARGIGATTIEKLVQASQAANTTLWEALKVVASPEA
                     ADPAARSVHGAARNGILELVHVIERTATGFQNGLDVVATTKGLIEDIRLYDDLRAAAP
                     SQAAAQRRIDNVTGLLNSLERWAQKGKGSRELAEYLRQLSLASDNDEKHDDSGDKVVL
                     TTLHGAKGLEFPVCFMIGLEEELLPHIRTLQPQATDVLDADHATDVSEERRLCYVGIT
                     RAQRKLYLTRACLRVSRGRAVPRTPSRFLLEIPDELLEIRDLAEEARQQVPKEEVANF
                     FANFAFDD"
     gene            8812..9351
                     /locus_tag="VFV99_19550"
     CDS             8812..9351
                     /locus_tag="VFV99_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061577.1"
                     /translation="MDRYTALVEALRKEIPGFRIVRKDQSRFHRAIHYGLMAITFGQM
                     RRYLDSYQTTIGKTVYVTSDWDETPADQRYVTLRHEAIHLRQFRKLTLPLMAIVYVLL
                     PLPMGLAYGRARLEQEAYAESIRAAAEVWGNDAVRSPEYRNYVIEQFTGASYGWMWPF
                     RRSLERWYDQILATLVEQR"
     gene            9368..11719
                     /locus_tag="VFV99_19555"
     CDS             9368..11719
                     /locus_tag="VFV99_19555"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5061578.1"
                     /translation="MSDPIVGIDLGTSNTVVAHASDPGNVQILADANGYKIHPSVVSF
                     HPTGGVVVGAAAKQRKVIDPKNTIYSVKRLIGRTFDSKEVQTAKQRSPFQIKDGANQL
                     PIIVTRGGEFAVPEISAIVLDHAKNVATHALGTPVSRAVVTVPASFNDAQRSGTATAG
                     SIAGLTVVRVLNEPTAAALAYGNLRSLRETIAVYDFGGGTFDITILKLSDQVYEVLGT
                     AGDTFLGGDDLDERLVDRMVEKFLMENRVDLRQNEVSMMRLRAVAEQVKIELSRRNRA
                     MVRIDEIAYGPKGKALDLQIEIKRDEFVRQVADIIDRTFPVCEEALKLANLSIDFIDD
                     VILVGGTTKIPYVRDQVAKFFSKAPRTDVNPEEAVAAGAALQANALDRVLSGRKPSRQ
                     IPVQSFEGETFGGETANKTVTYPEAPAGQSFIEMAMAQAAPDEDLLEPTGERPPGPRK
                     TQAYAQVEMPRPGRPGERAATRGDHHDVFEDRTPPPVSPTQPVLGRMTKQNITPPPAA
                     KGGPPVKPPPTPPPRTATQPVPVVRRPPTQQPLPKTITIPPSKSGPVMVPPVPPGPAS
                     TLRGIAPVHEEDEDTTGFPNLTTEKSITDLDLETSPASTAKGFAPHVPVATPQAKTIV
                     VEPPLAAPLPSAPQAVPVMAPPSPIVIDVTPRGLGIGTVAGFCEELIRRNSRVPTEIR
                     KLFTTSRDSQDSVRIIVCQGESRRLDNNTVIADLRLENLPRRPRGETSIEVTFALDAN
                     GILQVRARDAQTGAEQRASLDIVGTIQQGDVDAAREKLQALRR"
     gene            complement(11880..13112)
                     /locus_tag="VFV99_19560"
     CDS             complement(11880..13112)
                     /locus_tag="VFV99_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061579.1"
                     /translation="MRGYVVVSYALVGLLGACGGGEKKAPKKPVDTEVKPVAKPETEE
                     DRARKRQEEAEAIVPKGSSCLPVALKEENAPRLELGAAGRDALVCAFDQDPTRLLGPV
                     ACWKLDVMSGKLEYKDPEPLPGRGFAVLLDGKCARGYCLPTAPKSNKVYIAHNLDNSK
                     VAVLADDEVHIFNAASKAHESSFSIRGDKGVTNEPKAVHFVGETILVEGADEGPYSAV
                     WVFKTDGTKLGPITSLGSKDDKPVSTYHGSFSILDKTRVALGEKGMEFLNAYDVNTGA
                     RAKFVRKMPKLACKPAELDAYWVDGDKVTDKCKEGIQKASGPLMGATVVAGGKSLLVL
                     LRGDRLGQLAIMDNKSLAEKSKLDMPWCEGAEMKKEAAADEKKTEAKKPEEKKPSKKG
                     TTRGAVPADSSDPQEGGE"
     gene            13155..13754
                     /gene="hisB"
                     /locus_tag="VFV99_19565"
     CDS             13155..13754
                     /gene="hisB"
                     /locus_tag="VFV99_19565"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX5061580.1"
                     /translation="MARRTRIERNTKETQISVDLDLDGTGKRSIKTPVPFLSHMLDAF
                     ARHGVFDLEVKATGDTEIDGHHTVEDIGLVLGNAFEQALGDRAGIVRYGWATLPMDEV
                     MVTVALDFCGRPAFVWKVQGLEGKWIGDFDCELAKEFFAAFATRAQCNLHVNVHYGGN
                     AHHMIECIFKAFARAADAATRIDPRLGGAVPSTKGTLTE"
     gene            13751..14350
                     /gene="hisH"
                     /locus_tag="VFV99_19570"
     CDS             13751..14350
                     /gene="hisH"
                     /locus_tag="VFV99_19570"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5061581.1"
                     /translation="MIAILDVCSGNLRSVERAVAHVGGNVVVTRDPEAVRKADKLIVP
                     GQGAFGVFMRGLEERGLGEALRERIAAGTPYLGICLGLQVLFEHSEEGDCAGLGVLRG
                     SVKKLVPGAQALKIPHMGWNRVVAKQPVRGIADGTYFYFVHSYVVVPSDPSITALEAS
                     HGVPFCAAIQRDNLFACQFHPEKSQAVGQRLLKTFVEAA"
     gene            14347..15069
                     /gene="hisA"
                     /locus_tag="VFV99_19575"
     CDS             14347..15069
                     /gene="hisA"
                     /locus_tag="VFV99_19575"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5061582.1"
                     /translation="MKLYPAIDLLGGKAVRLEEGRRDRATVFHDHPPALVAELARDGA
                     DRLHVVDLDGAFAGAPQQSDLIREIVAASPLPVEVGGGIRDRAAIDRVLELGAAFVVL
                     GTAAVRSPALVEDACRAHPGRVIVAVDARDGIVAVDGWTASGDVTAIELGKRAASWGA
                     AALLYTDVSRDGLHVGPNVDVTAQLARAVSCEVIASGGVGSLDDLSRLREAGIAAVVV
                     GRAIYDRRFTVAEAARVAHGGT"
     gene            15069..15821
                     /gene="hisF"
                     /locus_tag="VFV99_19580"
     CDS             15069..15821
                     /gene="hisF"
                     /locus_tag="VFV99_19580"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5061583.1"
                     /translation="MLARRIIPCLDVKDGRVVKGINFVELRDAGDPVEQAAAYEAQGA
                     DELCYLDISASPEGHDTIVDIVRRTADQVFMPLTVGGGVRSVADAERLLEAGADKIAV
                     NTAAIRRPELVREIAERFGNQAIVVAVDAKRRDASWEVFSHGGRTPEGLDAIEWCRRA
                     ADLGAGEILLTSMDRDGTGRGYDLDLIRTVALAVTVPVIASGGVGKLADLADGLVAGA
                     DAVLAASIFHFGQHTIGEAKAFLIERGLAVRT"
     gene            15823..16911
                     /locus_tag="VFV99_19585"
     CDS             15823..16911
                     /locus_tag="VFV99_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061584.1"
                     /translation="MKVYRSFALALVLAVTGAAVASADPSSDESAAKSKKKSSKSSKK
                     KTTSKKPAKKRTSHHSRKRTKTPKPIPGVPQDTDVTPVTDPGAPDPSSDTAATDAAAT
                     DAATSAPTDPVATEPAIAPTDPTQVDLDNASTPEVTADATAPEPPPPKPAATVTATVT
                     PTPKPARRGPTWYVGLRGGPSVIDRRGYYEYSSNRQIYATERNVRLDLVFGRYLSRHI
                     SLGLALGTGPYPKFDAPDPAYDENTRFEVFPAHARLDVALHAGPFVVGVGAGGAYEQA
                     TGTFGVQDPDTFLVTMHTARFKRYGAMGTVDTGFQFQLGPLGLELLAEASAVKLMRGT
                     YEHGITTAGPSDREMCYEASLLLGVRLQ"
     gene            16951..17616
                     /gene="hisIE"
                     /locus_tag="VFV99_19590"
     CDS             16951..17616
                     /gene="hisIE"
                     /locus_tag="VFV99_19590"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX5061585.1"
                     /translation="MSVEPSFDEQPGGLVPCVVQDAERGTVLMLAWMNAEALKLTRET
                     GIVHFWSRSRQSLWKKGETSGNTLAVVELRIDCDHDAVLVRAKPAGPTCHTGATSCFF
                     HKDDGTDDDGVPAVTIIEQLDRVLQARRDSASGEKSYTKSLLDAGMPKIMAKIAEEHG
                     ELAAELPSGEDGKVIYETADLLFHVMVGLTARNIPIERVLAELARRFGTSGHTEKAAR
                     PKK"
BASE COUNT         3053 a         5829 c         5749 g         3075 t
ORIGIN      
        1 ccgaagaaag cggccgcgcc gcgtaagaag aaggtgaagg ccgctgcagg ctcggtgggc
       61 cttactgccg acgaagtcgc gtcaggttca ccgccggccg aggtcacgaa gctcgagaaa
      121 cagatcgaag ctgcggacgg caaggtgctc gcggtctacc gagaacccct aggtggtcat
      181 tgggttgcgc tcgctgcgct cccgctcgac aaggtcaaac cgacgccgta tcagcgcgag
      241 ctctccaagg cgcacgccga tcgtttggcg atggtgattc ccaaagtcgg gcgtttcctc
      301 gatccggtga ttgcggttcc ggatgctgcg ggcacgttca taacgccgaa cggcatgcac
      361 cggctcgcgg cgctcggtca gctcggcgcg aagacggtcg tcgcgctcgt gatgccggag
      421 cccgagatcg cgttccgcat tttggcgctc aacaccgaga aggcgcacaa cctgaaggac
      481 aaggcactcg aggtgattcg catggcgcgc gcgatcgcgg cggaagacga tcgcagcgaa
      541 gagaagatgt cgttcgagct cgagctaccg cagctcgtaa cgatcggcgt ctgctacgag
      601 aagaacccgc ggttctcggg tggcgcgtac aactcggtcg tcggcaagtg cgagtcgtgg
      661 tcgaccgatc cgatgagcaa gagcttgaag ctgcgcgagg cacacgcgga caagctgctc
      721 gagctcgacg agctggtcgg ccaggccgtg cagaagctga aggacgctgg ctggacgagc
      781 ggctacttga agccgatcgt cgtcgcgcgc atcaacccgc ttcgcttcgt gaaacaaggc
      841 aaagacgaga agagcgactt cgacaagacc atcgacaaga tgatcgacgg cgcgaagaag
      901 ttcgacgcct cgaaggtgaa ggcgcaggac gtcgtcctcg ccgccacggt tggcggcggc
      961 gaagacacga actagcgccc actagcgctc gcggttagaa gaagtagtag tggatgccgc
     1021 caccgatcgt ttgaccggtg atcgcgacac cgcttgcgtc catcgtgccg atcgagacgc
     1081 cgacctgcgc gctgatcgcg acgttcgcgg caaggaacaa ccggatctgc gcacccgggt
     1141 cgataaagag gccggtctgg cgatcggccc ccatcggggc atcgaggctg acgataccta
     1201 gcgacccgcc gatgccgaag tcggacatcg cggtcgagtg cacgtggtag tagaagcggc
     1261 cgccgatcgt gaagtcggtg ttgtccgggc cgtcaccatc ttcgaagccc aagaagccgc
     1321 cgacgtggaa cgctccggca tcgtaggtga gcgaggctcc gccgagcgcg aacggcgtga
     1381 tgggggtgat catgaattcg gcgccaacac cgatcgagcc ttcttgcccg cctgcgtgcg
     1441 cgacacccga ggccgtaagt aacgctgctg caactagagt ggtcttcagc atgcctgaat
     1501 gctcacccgc catggatcga caatcaagta tgctgcccgc gtgatctcgg cggagctgga
     1561 cctacacgac ttggacgcgc gccattggcg aaatgcgtgg cgtctgctcg tacctccccg
     1621 cgtgctcgac aagccgcgct gggcgctcgt catcatcgac ggcacgccgg acaacgtcgt
     1681 caaggtgatc atcgcgggcg cggatgcgcg cggctcgatc gatccggcga gcgcaccatt
     1741 gccaggagtc ggcgagaagg cgctcggcgc atatgcgaag cagctcggcg tcggctcggt
     1801 gatcgtcgtc gatcggcacg tggtgaagca gttgtcgtcg gagatcgaga gcacgttgcg
     1861 cttcgatcag gatggcatcg cgcagggtct cgtcgcgctc aaagcgctca agaagcacgc
     1921 cggcaagggc gtgtggaccg agccgccgct gctcgagctg ttgccgacac cggcgatgga
     1981 tccgatccag cgcacgttcg atctgctcgt gcccgatcgc agcgcactcg tcgcgtacgt
     2041 gatcgaagac gactgtcagc gcgtgcatac gtcgatcatc gcggtcaaag aggacggaga
     2101 tatcgtacgc gccgctacgc atcgcgcgat cgctgatctc gtgcccgaga ccaagctcgc
     2161 gctcgcgtgg accacgcagt acaagcgcgt gctcgacgcc gtcgaggagc ggttcgcgaa
     2221 gccgtcgatc gcgctattca tcgagcgcgg aacgttgatg aaggtgctga ccggtccgag
     2281 cgatcagctc gcacgcgagt acaacgcgaa gaacgtgatc atcgatccgg tgccggcctg
     2341 gctgctcggg ttgctcggcg gcgctgccgt cgctgcgatg gccggtcgtg ccggcaaggc
     2401 gctcgccgcg atgctgccaa ccgccacgcg agagcgcgcc agtgcgctcg tcgcccgtgc
     2461 tcaggatgta atgaaggaat caggagctca ccccttcgcg ctcctgggat tcgatccgat
     2521 cgaattgtgg tcccgtctca agcaccacta tcggccctga gatcgaccgc caagacgccg
     2581 ccggacgcca agatcagaga tccttttcgg ggcttggtgt ctgccggcgt cttggcggtt
     2641 caatctcttc gcagtgtctc ctgcgtggtc gttagcggat caccgggaat gtgctcgagc
     2701 aagccatcga gctccgcgta gcgctcgcca cagcgcttgc acttgagatc gtccggtagt
     2761 gtctcaccgc aattgcacac ccacccaacg cgacgcccgg gtgttccgat gaccaacgca
     2821 tgtggcggta cgtccgccgt cacgaccgcc ccagcaccga tcatcgcgta ctcgccaatc
     2881 gtcacgccgc agatgatcgt cgcgttggct ccgacggtcg cgcctttgca gacgagcgtg
     2941 cgcgcgtact cgtccttgcg ctggacgtga gcgcgcggat gcttgacgtt cgtgaacacg
     3001 cagcacgggc cgaggaacac gtcgtcctcg agcgtgacgc cgtcatagat cgagacgtgg
     3061 ttctggatcc ggcagccgtg accgatccgg accttgccga tgaagcagcc ctggccaatc
     3121 gagcaccgat cgccgatcca cgcgccctcc atgacatgaa cgaagtgcca cagcttggtg
     3181 tcgctaccga catgcgtttg cttgccgtcg acgatcgccg tggcgtggat gaagttgtcc
     3241 ggacgcggca tcgcgcatag gatgtgccta gctgtgccca agcgtccacc ccgtctggct
     3301 cgcgagcttg cggttccccg cgatttgcgg ggggcgggac cgacccgcgc tcacgccgcg
     3361 gacggtaacg agctctggca cgccgacgtg ctcgagctgc tcggcagact gccggacgga
     3421 agcgtcgacc tcgtcgtcac cgatccgccg tatgcgatcg cgaaggagag ctgggacgag
     3481 ttcgagtcgc tcgacgccta tgtcgattgg tgcgaccgct ggctcgccga ggtcgcgcgc
     3541 gtgctcgcgc cgcacggttc ggcctacgtg tgcgggttct ccgagatcct tgccgacgtg
     3601 aaggcgcgca gcgcgaagcg attcgcgaac tgccgctggc tcatctggta ctaccgaaac
     3661 aaagcgaacc tcggcaaaga ctggggccgc tcgcacgaat cgatccttca cctgcgcaac
     3721 gacgacgcgc gcatcgacgt agacgccgtg cgcgtgccct acaacggaca cacgacgaag
     3781 tacccggcgc gcgtgcaggc ggtgaattcg cagtacggcc gcggtgtgcg tcgcgatcgc
     3841 tgggagccaa atccgctcgg cgcgaagccg cgcgacgtca tcgagatccc ggtgatctgc
     3901 aacggcatgt ccgagaagac gccgcacgcg acgcagaagc ccgaggcact gatcgagcgc
     3961 ctgatcctcg cgtcgagtgc gaagggtcag ctcgtcgtcg atccgttcgt cggctcgggc
     4021 acgaccgcgg tcgttgctgc gaagctgggc cgaaaatggc tcgcgggcga tgccgacgcg
     4081 cgatacgtcg ggctcgcccg agacaggctc caggcctcag gctccaggct tcagccagaa
     4141 ccaagaacca agagatcctg acccgaaacg aacaacaccc gcacagtcat gcgggtgtcg
     4201 tccgaatctg ccccgaggcc tgaggcccga ggcccgaggt ccgaggcctg ctacagcgag
     4261 ggcgtcaggc ccatctcctt gaccaggtga tcggcgccgt cgacggcatc catcatccag
     4321 atcatgtagc gcgcgtcgac gtggatcgtg cgcgacgtct ggccgttgaa cagcacgtca
     4381 gacgacacgc cttccttgtt gccgtcgaac gcgagaccga cgagctcgcc cttgttgttg
     4441 agcgtcggcg agcccgagtt gccgccggtg atgtcgaggt cgctgaggaa gtcgaccggc
     4501 agctcgccgc cgagcgtgag gtcggcatac gggccgaact tcttcgcctt gatcgccgcg
     4561 agctcgttgg ccggcgcgtc gaacggcgcc ttgccggtgt tcttcgcgag gatctgcgac
     4621 gccgtcgtga acgcccagtc ctcctgcgcc ttcgactccg gcttgaacga cttcaccgtg
     4681 ccgtaggtga cgcggagcgt gccgttcgcg tcgggcgcga gtgcgccacc gaggactgcc
     4741 ttcaggcctt cggcgtacga cggcgtgacg agcacgagct cgccggcccg cgcgtcttcc
     4801 ttcttctcgt cggccttgac ggtcgaccag atgcgctgtg ccgccatcac gaacggatcc
     4861 ttcgacgcct tgatctgcgc catcgtgccc ttcgtgagca ggtcgatgcg gaccttctcg
     4921 tcctcgagct tggtgtcctt gtaccaggcg tcgagcgtct tatcgatcag cgcctcgtcg
     4981 atcttcgtgc ccttcttgac gccgagcagc gtggcgagcc acgggcgatc cgcctcgggg
     5041 agcgcgagcg agcgcatcag attgatgcgg aagctctcgc gatcgagcgt gcgatcgaac
     5101 gtacgcacga acgacttctg accggcgacc gagcgcggca gatcgcgctc ctggaaaccc
     5161 ggcttgcgat ccatatcttt cttcgcgcgc tgctcggccc agcgggtgag ggaaagcgcc
     5221 gtcgagagga acttgctgcc gccgaacgtc gcgccgcgct cgaagtcgac gcgcgcggta
     5281 cggaactcgt cggcgaggat gtgctcgagc ttctcgatgt tcgccttgtg gatctcgtta
     5341 cctggctgcg cggcccactc tttgaccttc ttgtcgagct cgtccttgcg ctgcaggatg
     5401 tcgctcttct tgatgccgtc gagctggccc tgaaacttct cgagaccgtt ctggatgaac
     5461 tgcttctggt tcgtcgcctt gatcgcggtc gcgttcttgt cgccgaggtg cgactccatc
     5521 aggttgtact tctgctgcag gtactcgatg tagaacggca gatgccactc gacgtcgtgg
     5581 tggatctccg aagccgtatc gaggcggctc gtcgagcccg gatagccagt gaccatcacg
     5641 aagtcgcccg gcttgagacc ggcggtcgtg accttgagcc agtgctgcgg atggaatgga
     5701 acgttgtctg ccgagaactc ggccggcttg ccgtccttgc cgacatacgc gcgatagaac
     5761 gaccagtcac cggtgtggcg cggccacgcc cagttgtcga tctcgccacc gtagttgccg
     5821 actgcgcgct gcggcacgta gaccaggcgc acgtccttga tctcgagcat ctcgatcagc
     5881 tggtacatgc tgccacggaa gaacgacgac acctggcaac gcagccacgg ccgatccttc
     5941 tcgcacgcag cgacctgctg cttgtagcgc ttctccgatt cctctttgcg cttgatcgga
     6001 tccttgatct tctcgaggcc gtcgcgcatc tccttcgtga tgtccttgta ggcctggacc
     6061 accatcacgc gctgcgacgg gctcgcgagg atctcgtcgg cgcgggtctt cgcgaggaag
     6121 ccgttctcga tgaggttctt ctcgggcgtc gagttgtagt tcagcgcgcc ctgcacgcag
     6181 tggtggttcg tgacgatgag accgtccggc gacacgaacg atgcggtgca gccaccgagc
     6241 gagacgatcg cggccagcgg atcgctgagc ggatcggaga gcgcttccgc gttcagcttc
     6301 acgcccatct tctggaacgt gtcgacgtgc tgcggcaacg tcatctgcat cggcatccac
     6361 atgccgcccg gatcgctgta ctgcctccgg aaatcgagtt gcggatcggc cggccgtggc
     6421 gctgcggctt cgtgctgctc ggcgctcgcg gtttccgacg attgcttgct cggacctagg
     6481 tcactcgtcg tgaccgcttg cttgttgctg cctccacagg cagcgaccaa caacccacac
     6541 aggatggcgt tcctcttcat gggcgccatg taccacagtc gatctatgga tccgattctc
     6601 ctggcgttcg actaccgctg acatctctgc gacttgtctt cacgcgacag ggtgcgacag
     6661 ccgcgggcag attgacgcta gtcgtcgaac gcaaaattgg cgaagaagtt ggcgacttcc
     6721 tctttgggca cctgctggcg cgcttcctct gcgagatcgc ggatctcgag cagctcgtca
     6781 gggatttcca gtaggaagcg cgacggcgtg cgcgggacgg cgcgcccacg cgagacgcgc
     6841 aggcacgctc gcgtcaagta gagctttcgc tgagcgcgag tgatccccac gtaacagagc
     6901 cggcgttcct cgctgacgtc ggtcgcgtga tcggcatcga gcacgtcggt cgcctgcggc
     6961 tgcagcgtgc ggatgtgcgg cagcaattcc tcctcgagac cgatcatgaa acagaccggg
     7021 aactcgagac ccttcgcgcc atgcagcgtg gtgagcacga ccttgtcgcc gctgtcgtcg
     7081 tgcttctcgt cgttgtcgga cgcgagcgag agctggcgca ggtactccgc gagctcgcgc
     7141 gagcccttgc ccttctgtgc ccagcgctcg agcgaattga gcaggccggt gacgttgtcg
     7201 atgcggcgct gcgcagcggc ctggctcggc gcggccgcgc gcagatcgtc gtagagccgg
     7261 atgtcctcga tcaggccttt cgtcgtcgcc acgacatcga gcccgttctg gaacccggtg
     7321 gccgtgcgtt cgatgacgtg aacgagctcg aggatgccgt tgcgcgcagc gccatgcacg
     7381 ctgcgtgcgg ctgggtctgc agcttcgggg ctcgcgacga ccttcagcgc ttcccacagc
     7441 gtcgtgttcg ccgcctgcga tgcctgcacg agcttctcga tcgtcgtcgc accgatgccg
     7501 cgcgctggat agttgatcac gcggcggagc gcgagctcgt cacgcgggtt gagcgcgacc
     7561 cgcaggtacg cgatgacgtc tttgacttcc ttgcgctcga agaactgctg cccgccgtac
     7621 atcacgtatg gaacctcggc cgcgcgcagc tcttcttcga ggaccttcga ctgcaggttc
     7681 gagcggtaga ggatcgcgat atcggagaag cgccggccat ccttgtggat gttccggatc
     7741 tctcgcgcga cccacttcgc ttcgtcctcg gcactctgcg cgacggcgtg cgtgatcttc
     7801 tcgccgtcac cgagctgcga ccacagcgtc ttgccgtggc gatccttgtt gttcgcgatc
     7861 accgtgttcg ccgcggcgag cacgttcttc gtcgagcgat agttttgctc gagcttgacg
     7921 atctttgcgc ccgggaacac gcgatcgaac tgcaggatgt tcgtcggatc ggcgccgcgc
     7981 cagctgtaga tcgattggtc gtcgtcgccg accacgacca ggttgccgtg ctgctcgacg
     8041 aggctctgca ccatgcgcaa ctgcgctttg ttggtgtcct ggaactcgtc gaccatcaca
     8101 tactggaagc gagacgccca gcgagcgcgc acatcctcgt cgcgctgaaa cagccgcacc
     8161 ggctcgacga tcagatcgtc gaagtcgaac gcggcgcacg cacgcagcgt ctcttgatac
     8221 ttcggataga cctggacggt catcgcatcg taatcgtcag cggggttgcc ctcgtactcg
     8281 tccggcgcga tgaacgcatt cttcgcgagc gagatccgcg tctgcaccgc tttcgcgtcg
     8341 tagcggcgat cgccgtcgtg caggtgacgc atcgcttcgc ggatcgcacc gagctgatcc
     8401 gattgatcgt agatcgcgaa gccgcgcggc agcccgagcg ccttgcgctc catccgcagg
     8461 atctggagcc cgagcgcgtg gaacgtgccg atcgtgagct cgcgcgcgag ctgcttgtcg
     8521 ccgagcaagt gcgcgatgcg ctcgcgcatc tcttcggccg ctttgttcgt gaacgtcacc
     8581 gcgcagatcg catgcgcggg cacgccttgc gcgagcaggt gcgcgatccg aaacgtgatg
     8641 acgcgcgtct tgcccgagcc ggcgcccgcg agcacgagca gaggacctcc accgtggctc
     8701 gccgcctccc gctgtggcgc gttgagcttg ctcagatcga ctgcagacac cgagcggacg
     8761 acgctacgga tctgggatcg atccgtcaac cgggagtgct acgctgcgcg cgtggatcgc
     8821 tacaccgccc tcgtcgaggc gctgcggaag gagatccctg gatttcggat cgtgcgcaag
     8881 gatcagtcgc gctttcatcg cgcgatccac tacggcctga tggcgatcac gtttggccaa
     8941 atgcgccgtt acctcgattc gtaccagacc acgatcggta agacggtcta tgtcacgtcc
     9001 gattgggacg agaccccggc cgatcagcgg tacgtcacgc ttcggcacga ggcgatccac
     9061 ttgcggcaat ttcgcaagct gacgcttccg ctgatggcca tcgtgtacgt gctcctgccg
     9121 ctgccgatgg ggctcgccta tgggagagcg cgcctcgaac aggaggctta cgccgagtcg
     9181 attcgcgccg cggccgaggt ttggggtaac gatgcggtac gcagccccga gtaccgcaac
     9241 tacgtgatcg aacaattcac aggcgcatcg tacggttgga tgtggccgtt ccgtcgaagt
     9301 ctcgagcgct ggtacgacca gatcctcgcc acactcgtgg aacaacgata acgtcgggga
     9361 tcgaacggtg agcgatccga tcgtaggcat cgacctggga acgtcgaaca cggtagtcgc
     9421 ccatgccagc gaccccggga acgttcagat cctcgccgat gccaacggct acaagatcca
     9481 tccgtcggtc gtgtcgtttc atccgaccgg cggtgtcgtg gtgggcgcag cggccaagca
     9541 gcgcaaggtc atcgatccga agaacacgat ctactcagtc aagcgcctga tcgggcgcac
     9601 gttcgattcc aaggaagtgc agaccgcgaa gcagcgctca ccattccaga tcaaggacgg
     9661 cgcaaaccag ctgccgatca tcgtcacgcg cggtggcgag ttcgcggtgc ccgagatctc
     9721 ggcgatcgtg ctcgaccacg cgaagaatgt cgcgacgcac gcactcggaa cgccagtgag
     9781 ccgcgcggtc gttaccgtgc ccgcaagctt caacgatgcg cagcgttcgg ggacggcgac
     9841 cgctgggtcg atcgcagggc ttaccgtcgt gcgcgtgctc aacgagccaa ccgcggcggc
     9901 actcgcgtac ggcaacctgc gtagcctccg cgagacgatc gcggtctacg acttcggtgg
     9961 cggcacgttc gatatcacga tcctcaagct gagcgatcag gtctacgagg tgctcggcac
    10021 cgcaggagac acgttcctcg gcggcgacga tctcgacgag cggctcgttg atcgcatggt
    10081 cgagaagttc ctgatggaga accgcgtcga cctccgccag aacgaagtgt cgatgatgcg
    10141 cctgcgcgcg gtggcagagc aggtgaagat cgagctgtcg cgccgtaacc gcgcgatggt
    10201 gcggatcgac gagatcgctt acggcccgaa gggcaaggcg ctcgatctgc agatcgagat
    10261 caagcgcgac gagttcgtgc gtcaggtcgc cgacatcatc gaccgcacgt tcccggtctg
    10321 cgaggaggcg ctcaagctcg cgaacctctc gatcgatttc atcgacgacg tcatcctcgt
    10381 cggcggcacg acgaagatcc cgtacgtgcg agatcaggtc gcgaagttct tctccaaggc
    10441 gccacgcacc gacgtcaatc ccgaggaggc agtcgctgcc ggtgccgcgc tacaggcgaa
    10501 cgcactcgat cgcgtgctgt cgggccgcaa accgtcgcgc cagatcccgg tgcagtcgtt
    10561 cgaaggcgag acgttcggcg gtgaaaccgc gaacaagacg gtcacgtacc ccgaagcgcc
    10621 cgccggccag tcgttcatcg agatggcgat ggcgcaggcc gcgcccgacg aggatctgct
    10681 cgaaccgacg ggcgagcggc cccccggtcc gcgcaagacc caggcgtatg cacaggtcga
    10741 gatgccgagg ccggggcgtc ccggcgagcg cgcagcgacg cgcggcgacc accacgacgt
    10801 gttcgaggac cgcacaccgc cgccggtctc tccgacacag ccggtgctcg gccggatgac
    10861 gaagcagaac atcacgccgc ctcccgccgc gaagggcggg ccgccggtga aaccgccgcc
    10921 gacgccaccg ccgcgcaccg caacgcagcc cgtgccggtc gtgcgccgtc cgccgacgca
    10981 gcaaccgcta ccgaagacga tcacgattcc gccgtcgaag tccgggcccg tgatggtgcc
    11041 gccggtgccg cccggtcccg cgagcacgct gcgcggtatc gcgccggttc acgaagagga
    11101 cgaggacacc accggctttc cgaacctgac gaccgagaag tcgatcacgg acctcgatct
    11161 cgagacatcc ccggcgtcga ccgcgaaggg cttcgcgccg cacgtcccgg tagccacgcc
    11221 gcaagccaag acgatcgtcg tcgagccacc gctcgcagcg ccactaccga gcgcgccgca
    11281 ggccgttccg gtgatggcgc caccgtcccc gatcgtcatc gacgtgacgc cgcgcgggct
    11341 cggcatcggc acagtcgcgg gcttctgcga agagctgatc cgccgcaact cgcgcgtgcc
    11401 gaccgagatc cgcaagctgt tcacgacgtc gcgcgacagt caggactcgg tacgcatcat
    11461 cgtgtgccag ggcgagtcgc gccggctcga caacaacacg gtgatcgcgg atctgcgcct
    11521 cgagaatctg ccgcgccgcc cgcgcggtga aacgtccatc gaagtcacgt tcgcactcga
    11581 cgcgaatgga atcctccaag tgcgcgcacg tgacgcgcag accggcgccg agcaacgcgc
    11641 gtcgctcgac atcgtcggca cgatccagca gggtgacgtc gacgcagccc gcgaaaagct
    11701 gcaggctctc cgccgctgag aaggcctccg gcctctggcc tcagacctcc gtggggcatc
    11761 agacgtcggg ccgcagacgt tcgcaccgcg ggctgctcgt tccggacctc gagcttcgcc
    11821 tcggcagctc gagcctgatg cctgcgaccc agcctgaagc ctgaagcccg aggcctgagc
    11881 tactcgccgc cttcttgcgg atcgctcgaa tcagccggaa ccgcgccgcg cgtcgtgccc
    11941 ttcttcgacg gcttcttctc ttccggcttc ttcgcttcgg tcttcttctc gtcggcggcc
    12001 gcttccttct tcatctcggc gccttcgcac cacggcatgt cgagcttgct cttctcggcg
    12061 agcgacttgt tgtccatgat cgcgagctgg ccgagccgat ctccgcgcag cagcacgagc
    12121 aggctcttgc caccggcgac gaccgtcgcg cccatcagcg gccccgacgc cttctgaatg
    12181 ccttctttgc acttgtcggt gaccttgtcg ccgtcgaccc agtacgcgtc gagctcggcg
    12241 ggcttgcacg cgagcttcgg catcttgcgg acgaacttcg cgcgtgcgcc ggtgttgacg
    12301 tcgtacgcgt tgaggaactc catgcccttc tcgccgagtg cgacacgcgt cttgtcgagg
    12361 atcgagaacg acccgtgata cgtcgagacc ggcttgtcat ccttgctacc gaggctcgtg
    12421 atcggaccga gcttcgtgcc gtcggtcttg aacacccaga ccgcggagta cggcccttcg
    12481 tccgcaccct cgacgaggat cgtctcgccg acgaagtgga ccgccttcgg ctcgttcgtc
    12541 acgcccttgt cgccgcggat gctgaacgac gactcgtgcg ccttcgaggc tgcgttgaag
    12601 atgtgcacct cgtcatcggc gagcaccgcg accttcgagt tgtcgagatt gtgcgcgatg
    12661 tacaccttgt tggacttcgg tgcggtcggc aggcagtagc cccgcgcgca cttgccgtcg
    12721 agcagcaccg cgaagccacg gcccggcagc ggctcgggat ccttgtactc gagcttgccg
    12781 ctcatgacgt cgagcttcca gcacgcgacc ggaccgagca gccgcgtggg atcctgatcg
    12841 aaagcgcata cgagtgcgtc gcggcccgcg gcaccgagct cgagccgagg cgcgttctct
    12901 tccttgagcg cgactggcaa gcacgacgaa cccttcggga cgatcgcctc ggcctcttcc
    12961 tgacgcttcc tggcgcggtc ttcctcggtt tccggtttgg caaccggctt cacttcggtg
    13021 tcgaccggct tcttcggagc cttcttctcg ccgccgccgc acgcaccgag tagccccacg
    13081 agcgcataag aaacgactac gtagcccctc atgccgatcg ttgtaccgtc ggcgacaggc
    13141 tgatacaagg agctgtggca cgtcgtacgc gcatcgagcg caacacgaag gagacgcaga
    13201 tcagcgtcga cctcgatctc gacggcaccg gcaagcggtc gatcaagacg ccggtcccat
    13261 tcctctcgca catgctcgac gcgttcgcga ggcacggcgt gttcgacctc gaggtcaagg
    13321 cgaccggcga caccgagatc gacggtcacc ataccgtcga ggacatcggg ctcgtgctcg
    13381 gcaacgcgtt cgaacaggcg ctcggcgacc gcgccggcat cgtgcgctac ggctgggcga
    13441 cgctgccgat ggacgaagtc atggtcacgg tcgcactcga tttctgcggc cgaccggcct
    13501 tcgtgtggaa ggtgcagggg ctggaaggca agtggatcgg cgacttcgac tgcgagctcg
    13561 cgaaagaatt cttcgcggca ttcgcgaccc gcgctcagtg caatttgcac gtgaacgtcc
    13621 attacggcgg aaacgctcac cacatgatcg aatgcatctt caaggcattc gctcgcgcag
    13681 ccgatgcggc gacgagaatc gatcctcgcc tcggcggtgc ggtaccatcg acaaagggta
    13741 cgctcacgga gtgatcgcaa tcctcgacgt ctgttctggc aacctgcgct cggtcgagcg
    13801 tgcggtcgct cacgtcggag gcaatgtcgt cgtcacgcgc gatcccgaag cggtccgcaa
    13861 agcggacaag ctgatcgtgc cagggcaggg cgcgttcggc gtgttcatgc gagggctcga
    13921 ggagcgcggg ctcggcgaag cgctgcgcga gcggatcgcg gccggcacgc cgtacctagg
    13981 catctgcctg ggactgcagg tgctattcga gcacagcgaa gaaggcgact gtgcgggtct
    14041 cggcgttctc cgcggttcgg tcaagaagct cgtccccggt gcgcaggcgc tcaagattcc
    14101 ccacatgggg tggaaccgcg tcgtcgcaaa acagccggta cgcggcatcg ccgacggtac
    14161 atacttctat ttcgtgcact cgtacgtcgt cgtgccgagc gatccgagca tcactgcgct
    14221 cgaagcgagc cacggcgttc cattctgcgc ggcgatccag cgcgacaacc tgttcgcgtg
    14281 tcagttccac cccgagaaga gccaggccgt cggtcagcgg ctgttgaaga cgttcgtgga
    14341 ggcagcgtga agctgtaccc cgcgatcgat ctgctcggcg gcaaagcggt gcgcctcgaa
    14401 gagggacgcc gcgatcgcgc gaccgtgttt cacgatcatc cgccggcact cgtcgccgag
    14461 ctcgcgcgcg atggggcaga ccgcttgcac gtcgtcgatc tcgacggcgc gtttgccggc
    14521 gcgccacaac aatcggatct catccgcgag atcgtggccg cgtcgccgtt gccggtcgaa
    14581 gtcggtggcg gcattcgcga ccgcgctgcg atcgatcgcg tgctcgagct cggtgctgcg
    14641 ttcgtcgtgc tcggcaccgc ggcggttcgc tcaccggcgc tcgtcgaaga tgcatgtcgc
    14701 gcccatcccg gccgcgtgat cgtcgcggtc gatgcgcgcg acggcattgt cgcggtagac
    14761 ggctggacgg cgagtggcga cgtcaccgcg atcgagctcg gcaagcgggc cgcgagctgg
    14821 ggcgctgccg cgctgctgta caccgatgtc tcgcgcgatg gtttgcacgt cgggccgaac
    14881 gtcgatgtca ccgcacagct cgcccgcgcg gtcagctgcg aagtgatcgc gtcgggaggc
    14941 gtcggcagcc tcgatgatct gtcgcgactg cgcgaagccg gcatcgccgc cgtcgtcgtc
    15001 ggccgcgcga tctacgatcg ccgcttcacg gtcgccgagg ccgcgcgcgt cgcgcatgga
    15061 ggtacgtagt gctcgcgcgc cggatcattc cgtgtctcga tgtcaaagac ggtcgcgtcg
    15121 tcaaaggcat caacttcgtc gagctgcgcg acgctggtga tccggtcgaa caagccgcgg
    15181 cctacgaagc gcaaggtgcc gacgagctgt gttacctcga catctcggcg tcgcccgagg
    15241 gccacgacac gatcgtcgac atcgtgaggc gcacggccga ccaggtgttc atgccgctca
    15301 ccgtcggcgg tggcgtgcgc tcggtcgcgg acgccgaacg cctgctcgaa gcgggcgcgg
    15361 acaagatcgc ggtcaacacc gccgcgattc gccgccccga gctcgtccgc gagatcgccg
    15421 agcgcttcgg caatcaggcg atcgtcgtcg ccgtcgacgc gaaacggcgc gacgccagct
    15481 gggaagtgtt cagtcacggt ggacgcacgc ccgagggact cgatgcgatc gaatggtgcc
    15541 gccgcgcagc cgacctcggc gcaggtgaaa tcctgctgac gagcatggat cgcgacggca
    15601 ccggccgcgg ctacgacctc gatctcatcc gcacggtcgc gctcgcggtc accgtgccgg
    15661 tcatcgcgtc gggtggggtc ggcaagctcg ccgatctcgc agacggtctc gtcgccggtg
    15721 ccgatgcggt gctcgcggct tctatctttc acttcggtca gcacacgatc ggtgaagcga
    15781 aggcattcct gatcgaacgc ggcctggcgg tgcggacctg acgtgaaggt ctatcgatcc
    15841 ttcgcgctcg cgctcgtgct cgccgtgacc ggtgcggctg tcgcaagcgc cgatccgagc
    15901 tccgacgaga gcgcagcgaa gagcaagaag aagagctcga agagctcgaa gaagaagacc
    15961 acgtcgaaga agccggcgaa gaaacgcacg agccatcact cgcgcaagcg gacgaagaca
    16021 cctaaaccga tccccggtgt gccgcaagac accgacgtca cgcccgtcac cgacccgggc
    16081 gcgccagatc cgagctccga cacagccgcg accgacgcag ccgcgaccga cgcagctacg
    16141 tccgcgccga ccgatccagt cgcgaccgag cccgcgatcg cgccgaccga tccgacgcaa
    16201 gtcgatctcg acaacgcgag cacgcccgag gtcaccgccg acgcgacggc cccagagcca
    16261 ccgccaccga agcccgcggc gaccgtaacc gcgaccgtca cgcccacgcc caagccggcc
    16321 cgccgtgggc ctacctggta cgtcggcctg cgcggtggac cgtcggtgat cgatcgccgc
    16381 ggctactacg agtacagcag caaccggcag atctacgcga ccgaacgcaa cgtgcgactc
    16441 gatctcgtgt tcggccgcta cctttcgcgt cacatctcgc tcggcctcgc gctcggcacc
    16501 gggccgtacc cgaagttcga cgcgccggat ccggcgtacg acgagaacac gcggttcgag
    16561 gtgttccccg cgcatgcgcg gctcgacgtc gcactgcatg ctggtccgtt cgtggtcggc
    16621 gtcggggccg ggggtgccta cgagcaagcg acaggcacgt tcggcgtgca ggaccccgac
    16681 acgttcctgg tcacgatgca caccgcacga ttcaagcggt acggtgcgat gggcaccgtc
    16741 gacacgggat ttcagttcca gctcggcccg ctcgggctcg agctgctcgc tgaagccagt
    16801 gcagtgaagc tgatgcgcgg cacgtacgag cacggcatca cgaccgccgg gccatccgat
    16861 cgggagatgt gttacgaggc gtcgctactc ctcggcgttc ggctgcagta atcaagttga
    16921 cacccggatc cgcgctggcg aatgtagggc gtgtctgtcg agccgtcgtt cgacgagcag
    16981 ccgggaggcc tcgtgccgtg cgtcgtgcag gatgccgagc gcggcaccgt gctgatgctc
    17041 gcgtggatga acgccgaggc gctgaagctg acgcgcgaga ccggcatcgt ccatttctgg
    17101 tcacggtcgc gccagtcgct gtggaagaaa ggcgagacaa gcggcaacac gctcgcggtc
    17161 gtcgagctgc ggatcgactg cgatcatgac gcggtgctcg tgcgcgcgaa gccggccggg
    17221 ccgacgtgtc ataccggcgc gacaagctgc ttctttcaca aggatgacgg caccgacgac
    17281 gacggagtcc cggctgtgac gatcatcgag cagctcgatc gggtgctgca ggcgcgtcgc
    17341 gattcggcga gcggagagaa aagctatacg aagtcgctgc tcgatgcggg catgccgaag
    17401 atcatggcga agatcgccga ggagcacggc gagctcgcgg cggaattgcc gagtggcgag
    17461 gacggcaagg tcatctacga gaccgccgat ctgctgtttc acgtgatggt cgggctgacc
    17521 gcgcggaaca tcccgatcga gcgcgtgctc gccgagctgg cgcgcaggtt cggaacgagc
    17581 ggtcacaccg agaaagccgc gaggccgaag aagtgatcga cctgctcgca cccggcggtg
    17641 ggctcgagcg tgcgattcca tactacgaag accgtgccga gcagcgcacg atgtccgcgg
    17701 ccgtcg
//
LOCUS       DASUFX010000487        10685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_772470,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000487 DASUFX010000000
VERSION     DASUFX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772470"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFV99_19595"
     CDS             complement(<1..561)
                     /locus_tag="VFV99_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061586.1"
                     /translation="MADWRLGARVIAIAIAVAGCCPLPEADEPKVPPPPKQQAPLYTI
                     AKEIPPAKMGHFEHQPPKAPVVPTNREMLKEPPLVPPAAPKNELPTRRETAAPPSAPT
                     AQLDRLPDDVVMKLAETGRAAFVRCFKKAYQADPNQLSFKVRVHVELDGDGALTTANA
                     DTTDQALAGCLVRSLGWLKFPAAGKPV"
     gene            725..1777
                     /locus_tag="VFV99_19600"
     CDS             725..1777
                     /locus_tag="VFV99_19600"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX5061587.1"
                     /translation="MRVLPLLVLAACGDPATTVDATRSSDANPPNDTSLRDAAVPAWL
                     AGVNLSVAEFGEQNLPGTYGTDYIYPTTAEVDYFKSIGMNTLRVPFRWERLQRSLNAA
                     FDATEKSRLDTFVAYATGAGMTVILDPHNYARYGNNLVGSTAVPRSAFADFWGKLGAA
                     YSNNPRVVFGLVNEPHDMVTEDWLAAANDAIAAIRAAGAANLILVPGNAWTGAHAWLD
                     NYYGTPNGTVMLGVVDPMNHFAFEVHQYLDSDSSGTSATCVSATIGSQRIAGFTSWLK
                     TNGYRGFLGELGAGNNTTCASAVDDMLDVLDANRDVWMGWAWWAAGPWWGNYMFSIEP
                     DPGDKPQIAWLTPHMN"
     gene            complement(1774..2550)
                     /locus_tag="VFV99_19605"
     CDS             complement(1774..2550)
                     /locus_tag="VFV99_19605"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5061588.1"
                     /translation="MTTLAMALPTNDDDATGLTIDELAATSKVPSRTIRFYQSRGALM
                     NPEIRGRVAYYGQQHVERLKLIAQLQDRGLRIDAIGDLMKRIDRGEVDLAEWLGVEEE
                     MQTPWATDQARTLNETELYELVGSRRPGLLADLQRAKIVERKGDVYFVASPALLAIAM
                     KLEGVGIDLDTAHAASAILRKHLSRAVGDLVDLFLDRVKDGHISIAQSGKLFETFRVV
                     GVDSVRVLFAREMEHALRKLLSSGKLASLSARARGRRRDR"
     gene            complement(2547..3695)
                     /locus_tag="VFV99_19610"
     CDS             complement(2547..3695)
                     /locus_tag="VFV99_19610"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX5061589.1"
                     /translation="MSHQLPVAIDDAAIEEFGRELDAIQREVKASLGEADRRYIKRMI
                     KIQRGLAVGGRVLIFASLPLMPAFPLFLGVIGAGTAALGLSKILENMEIGHNVIHGQW
                     DWMQDPEIHSSTWEWDNVCPADQWKHSHNVVHHTWTNVMGKDRDVGYEILRINEAQPW
                     KPVYLAQPFYNALLAVMFQWGVAMHDIDLTRVRKGELKFRDIRHQLAGIGKKARKQLL
                     KDYVLWPLLAGPLFLPVILANLTANLMRNLWSYAIIFCGHFPDGAQVFTEAQVEGETR
                     GRWYLRQTLGSCNIEGGPTFHVLSGNLSHQIEHHLFPDMPSNRYPEVAPRVRALCARF
                     GIPYNTGSFARQLGTTMRTILRLALPTKSKTKTQPEPKTETKTEALAA"
     gene            complement(3711..4781)
                     /locus_tag="VFV99_19615"
     CDS             complement(3711..4781)
                     /locus_tag="VFV99_19615"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX5061590.1"
                     /translation="MKNVSTSLWTRLADIAGLVATPLVPSHYIALVSPLAATHTRQAR
                     VEAVWDETADTRTLTLRPGRGWQPHRAGQHVRIGLAIDGRIATRTYSISSPPERRDGC
                     ITITIKAQGKMSRALVRDLAVGSYVTIGLPEGEFVLPYVLPAQLAFITAGSGITPVMS
                     MLRSLAARGELPDIAHVHYARHDRDVIFGDELRALAAAHPRYRLTIVHTHDDPRRFSR
                     ERLADLVPDWRARETWACGPQGLLAAITDCYTGGPALHIERFTAAFAPAPADATDGTV
                     HFTTTQRKTKSDGRTPLLRIAEDAGVYAPHGCRMGICHTCDTTLLAGCVRDLRTGERI
                     DQPGTRIQVCVCAAAGDVELAL"
     gene            4891..6021
                     /locus_tag="VFV99_19620"
     CDS             4891..6021
                     /locus_tag="VFV99_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX5061591.1"
                     /translation="MRPLVLYADDEANNRIVFAQSFGNQFDVITVPDGAAALALLETR
                     EAAVLVTDMRMEGLSGEELLRIAKDRHPRTIRIVVTAYSDIDRILRALNEGLVARYII
                     KPWEHAEITQVLRWACEAWSLSTNAAALHNRLAETERLVASASSFVHDVKSPIMSLLA
                     NTDHLHELAGDVPALRAALAAVPLADEHRRALAFILDELLPISEEMQTSTRRIQELVE
                     TVAARPAPAPIIDPLQIVRHVMAVCQPLAAQLSASLDYEGPGALPRIRMSASELSEVL
                     INLVANGAQAVGARGTTGHVSITAHLLDGMLELQVRDDGVGIPAAAMSRVGTPFYTTR
                     IGGMGLGVAQCQRLVAIAGGRFSIDSQPGVGTIATISIPTAA"
     gene            6093..6461
                     /locus_tag="VFV99_19625"
     CDS             6093..6461
                     /locus_tag="VFV99_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061592.1"
                     /translation="MKRILLMDDSEMFLEVTCTALKAVGYDVICANDLAQLAEARKQG
                     PSDLVLMDVQMPEAFGDDIALTLRHVYGISAPIYLLSSLAETDLADRVEWAGIDGFIS
                     KQLGVEAIISRVRSIVPVES"
     gene            6458..7210
                     /locus_tag="VFV99_19630"
     CDS             6458..7210
                     /locus_tag="VFV99_19630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEX5061593.1"
                     /translation="MSEHDDDDGSEQRKHARVPLRLIVEYEDADDFVGDYTENLSAGG
                     TFIHTTRLLERDTAVQLVLSFPGLLQPIALEGIVRWSRGGKQPGVGLEFVPNQDYAKL
                     ETLVRLVEARDPRAVARVVRVLLAEDNPHIAELICNGVGASAKRTFGDALAFYFATAE
                     NGAVAVELLKTAPFDVAIIDLYLPVLDGAKVIENARSDLGLTKLPIIAISAAGDMAKR
                     SALDAGANVYLSKPMRLREVLESMRRLVPMSA"
     gene            7250..7630
                     /locus_tag="VFV99_19635"
     CDS             7250..7630
                     /locus_tag="VFV99_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061594.1"
                     /translation="MAQRKILLIDDSEVSLHFASRILGKAGFDVRTTLDVHDLQSVLG
                     GWRPDIILTDVDMPGVTGPELCRMLKSSYETAHVPVVLFSAVPSRELETMARECEADG
                     FLSKANGLERLPQELELLIETALF"
     gene            complement(7639..9582)
                     /locus_tag="VFV99_19640"
     CDS             complement(7639..9582)
                     /locus_tag="VFV99_19640"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX5061595.1"
                     /translation="MRLAADSRRRAGYALRVVAIAIAYFAVGELALRVHKLAGVYSLI
                     WAPPGIGFALVLLYGLRWWPGLFLGAFASVIIRNPPEVAFIVATTEVLEAMVGYYVLR
                     KLLHFRPQLERVRDVVSFAAVDATISAADAAFGAAALLATGFNGHADFVNLWSTWWWI
                     NLSGDLIVCPVLFTWALPTSLPKTFEGRWRRLETIAFVALGLLLVLLVLTGWLPSWLR
                     ASRTYYLLPLLLWAGVRFGPRGAATASFVVSLSAIVAAALGVAPFAAQLDLQIFVALS
                     SVTTLMLSALAQERVRAVQRTAAIQQTALDAIFTIDVHGRILEVNPAAEQLFSIRKLE
                     VLGNDLAAVIIPPRLRAAYYQGLQKYYREGSGPVIGSRYRTIGWRAQDSHEFPVEVAV
                     TVASTGDEPVLTGFVRDISAEQAAEAARQEANEKLERTVATRTAELQSSLRAKDLLLR
                     ELHHRVKNNLQVMSSLLNLQIIAEPSERTRRRLVDNQSRIQSMALVHQLLYQSMEVAH
                     VDFGEYLQELIQRLAAAYGEVAEHVSTDVDVEHVQLDIDRAILCGMLVNELVTNALTH
                     AFPGGRAGTVHVRFAQQEASLAITVTDDGVGISGPLNLATPKTFGLQIIVTLARQLDG
                     QIEQLPGPGTSICVRFPAANTVA"
     gene            complement(9720..>10685)
                     /locus_tag="VFV99_19645"
     CDS             complement(9720..>10685)
                     /locus_tag="VFV99_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061596.1"
                     /translation="VGIARRFGQFVEAAGTPGYVAPEVIAGQEATVRSDVYGLAATAY
                     ALLTLAHPWGDGDDVIARQISGEPVPPPSSHRAELAEIDALFADALAGDPARRPASAS
                     AFAASLRASLAIETLARKPDGPRWNGNVVLPRRAIARTRGVVFRSVARALGVREAQKL
                     RDTIGGAHPDLAAALTDTAPLAWMPTELFSRLLAIAPQHTDRDGPRLARDIARATVRA
                     SFRRFFPASSATLVPERTLSAIRNVWGQYQSWGVVTSMPVNPSETVVRIADALPDAEL
                     CAWTDGLLEQLIVLSGGRGPIVDHEACISRGDAACLYRVTWNRQA"
BASE COUNT         1860 a         3358 c         3601 g         1866 t
ORIGIN      
        1 gaccggtttg cccgcggccg ggaacttcag ccagccaagc gagcgcacga gacagcctgc
       61 gagcgcttga tcggtcgtgt cggcgttcgc cgtcgtgagc gcaccgtcac cgtcgagctc
      121 gacgtgcacg cgcaccttga acgagagctg gttcgggtcg gcttgatatg ccttcttgaa
      181 gcagcgcacg aatgcggcgc gcccggtctc ggcgagcttc atcacgacgt cgtcgggcaa
      241 acgatcgagc tgcgcggtcg gcgcacttgg cggtgccgcg gtttcacgtc gcgtcgggag
      301 ctcgttcttc ggcgcagcgg gcgggacgag cggcggctcc ttgagcatct cgcggttcgt
      361 tggcaccacg ggggccttcg ggggctggtg ctcgaagtgg cccatcttcg cgggtgggat
      421 ctctttcgcg atcgtgtaga gcggcgcctg ctgcttcggc ggcggcggca ccttgggctc
      481 gtccgcttcg ggcagcgggc agcagcctgc aaccgcgatc gcgatcgcga tgacccgtgc
      541 ccccaggcgc caatcggcca catatgatcg atgcacgctc gggcatgtat gttcaagcga
      601 acgtgagtta gcgggcctca cgaagtggtc gcgcggagtt aggtgtgtgt ggcgagagcc
      661 gctcagtatt cgcgtttacg aaagccgcgt cgggtgcgcg tctgccggcg ttatgatcga
      721 gttcatgcgg gttttgcccc ttctggttct cgcggcgtgc ggcgatccgg ccaccaccgt
      781 cgacgccacc cgcagctcgg atgccaaccc gccgaatgac accagcctcc gcgatgcggc
      841 ggtcccggcg tggctcgccg gcgtgaatct gtcggtcgcc gagttcggcg agcagaacct
      901 gccgggcacc tacggaaccg actacatcta cccgacaact gccgaggtcg attatttcaa
      961 gtcgatcggc atgaacacgc tgcgggtgcc gtttcgctgg gagcgcctgc agcgcagcct
     1021 caacgccgcg ttcgatgcga ccgagaaatc gcgtctcgac acgttcgtcg cgtacgccac
     1081 cggcgcgggc atgaccgtca tcctcgatcc gcacaactac gcgcgctacg gcaacaacct
     1141 cgtcggctcg acggcagtgc cgcgcagcgc gtttgccgac ttctggggca agctcggcgc
     1201 cgcgtacagc aataatccgc gcgtcgtgtt cggcctcgtc aacgagcccc acgacatggt
     1261 caccgaggat tggctcgccg ccgcgaacga cgccatcgcg gcgatccgcg ctgccggcgc
     1321 ggcgaacttg atcctcgttc ccggcaatgc gtggaccggc gcgcacgcat ggctcgacaa
     1381 ctactacggc acgccgaacg gcaccgtgat gctcggtgtc gtcgatccga tgaaccactt
     1441 cgcgttcgag gtccaccagt atctcgacag cgattcgtcg gggacgagtg caacgtgtgt
     1501 gtcggcgacg atcggcagcc agcggatcgc cggcttcacg agctggctga agacgaatgg
     1561 ctatcgcggg tttctcggcg agctcggcgc gggcaacaac acgacgtgcg cgagcgcagt
     1621 cgacgacatg ctcgacgtcc tcgacgcgaa tcgcgacgtc tggatgggct gggcgtggtg
     1681 ggcggccggg ccgtggtggg gcaactacat gttctcgatc gagccggatc cgggcgacaa
     1741 gccgcagatc gcctggctca cgccacacat gaactagcgg tcacgccgtc ggccccgcgc
     1801 gcgcgccgat agcgacgcga gcttgcccga ggagagcagc ttgcggagcg cgtgctccat
     1861 ctcgcgcgcg aacaacacgc gcaccgagtc aacaccgacg acgcggaacg tctcgaagag
     1921 cttgccggac tgcgcgatgc tgatgtggcc gtccttgacg cgatcgagaa acagatcgac
     1981 gaggtcgccc accgcacgcg acagatgctt gcgcaggatt gccgacgcgg cgtgcgcggt
     2041 gtcgagatcg atcccgacgc cctcgagctt catcgcgatc gcgagcagcg cggggctggc
     2101 gacaaaatag acgtcgcctt tgcgctcgac gatcttcgcg cgctgcagat cggcgagcag
     2161 accggggcgc cgcgacccga cgagctcgta cagctcggtt tcgttgagcg tgcgcgcttg
     2221 atccgtcgcc cacggcgtct gcatctcttc ttcgacgccg agccactccg cgagatcgac
     2281 ctcgccacga tcgatgcgct tcatcagatc gccgatcgca tcgatgcgca ggccgcgatc
     2341 ctggagctgg gcgatcagct tgagccgctc gacgtgctgc tggccgtagt acgcgacgcg
     2401 gccccggatc tccgggttca tcagcgcgcc acgcgactga tagaaacgga tcgtgcggct
     2461 cgggaccttc gaggtcgcag cgagctcgtc gatcgtgaga ccagttgcgt cgtcgtcgtt
     2521 cgtcggtagt gccatcgcga gggtcgtcat gccgccagcg cctcggtctt ggtctcggtc
     2581 ttcggttcag gctgggtctt ggtcttgctc ttcgtgggca gggcgaggcg gaggatcgtc
     2641 cgcatcgtcg taccgagctg gcgggcaaac gagccagtgt tgtacgggat tccgaaccgc
     2701 gcgcaaagcg ccctgacgcg cggagcgacc tcggggtagc gattgctcgg catgtcgggg
     2761 aacaggtggt gctcgatctg atggctgagg ttgccgctca gcacatggaa tgtcggaccg
     2821 ccctcgatgt tgcacgagcc gagcgtctgg cgcagatacc agcgcccgcg cgtctcgccc
     2881 tcgacctgtg cctcggtgaa cacctgtgcg ccgtcgggga agtggccgca gaagatgatc
     2941 gcgtacgacc acaggttgcg catcaagttt gcagtgaggt tcgcgaggat gaccggcagg
     3001 aacagcgggc cagcgagcaa cggccacagc acgtagtcct tgaggagctg cttgcgcgcc
     3061 ttcttgccga tgcccgcgag ctggtggcgg atgtcgcgga atttgagctc gcccttgcgc
     3121 acgcgcgtga ggtcgatgtc gtgcatcgcg acaccccact ggaacatgac cgcgaggagc
     3181 gcgttgtaga acggctgcgc gaggtagacc ggcttccacg gctgcgcttc gttgatgcgc
     3241 aggatctcgt agccgacgtc gcgatccttg cccatcacgt tcgtccacgt gtggtggacg
     3301 acgttgtgcg agtgcttcca ttgatcggcg ggacacacgt tgtcccattc ccacgtcgag
     3361 gagtggatct cgggatcttg catccagtcc cactggccat ggatcacgtt gtggccgatc
     3421 tccatgttct cgaggatctt ggacaggccg agcgctgcgg tgccggcgcc gatcacgccg
     3481 aggaacagcg ggaacgctgg catgagcggc aagctcgcga agatcagcac gcggccgccg
     3541 acggcgagtc cgcgctggat cttgatcatt cgcttgatgt agcggcgatc agcctcgccg
     3601 aggctcgcct tgacctcgcg ttgaatcgcg tcgagctcgc ggccgaattc ttcgatcgcg
     3661 gcatcgtcga tcgcgacggg tagttgatgt gacatgacaa atcctttcgt tcagagtgcg
     3721 agctcgacgt cgccagccgc tgcgcacacg caaacttgaa tgcgtgtgcc aggctggtcg
     3781 atgcgctcgc cggtccgcag gtcgcggacg catcctgcga gcaacgtcgt gtcgcaggtg
     3841 tggcagatgc ccatccggca tccatggggt gcgtagacgc ctgcatcttc agcgatgcgg
     3901 agcagcggcg tgcggccgtc gctcttcgtc ttgcgttggg tggtggtgaa gtgaaccgtg
     3961 ccgtcagtcg cgtcggcagg ggcaggcgcg aatgccgcgg tgaagcgttc gatgtgaagc
     4021 gcggggccgc cggtgtagca atcggtgatc gcggcgagca ggccctgtgg tccgcatgcc
     4081 caggtctcgc gtgcgcgcca gtcgggcacg aggtcggcga ggcgttcgcg cgagaaccgg
     4141 cgcgggtcgt catgcgtatg gacgatcgtc aggcggtagc gcggatgcgc ggctgccagc
     4201 gcgcgcagct cgtcgccgaa gatcacgtcg cggtcgtggc gcgcgtagtg gacgtgcgcg
     4261 atgtcgggga gctcgccgcg ggcagcgagc gagcggagca tgctcatcac gggcgtgatg
     4321 ccgctgccgg ccgtgatgaa cgcgagctgc gcgggcagga catacgggag gacgaactcg
     4381 ccttcgggaa ggccgatcgt cacgtagcta ccgaccgcga ggtcacggac gagtgcgcgg
     4441 gacattttgc cctgcgcctt gatggtgatc gtgatgcagc catcgcggcg ctcgggcggc
     4501 gacgaaattg aataggtgcg cgtcgcgatg cggccgtcga tcgcgaggcc gatgcggacg
     4561 tgctggccgg cgcgatgggg ctgccagccg cggccgggcc gcagcgtcag cgtacgggta
     4621 tcggcggtct cgtcccagac cgcttcgacg cgggcctggc gcgtgtgcgt cgccgcgagc
     4681 gggctgacga gcgcgatgta gtgcgacggt acgaggggcg tcgcgacgag gccggcgatg
     4741 tccgcgaggc gggtccagag gctcgttgag acattcttca ttgagccaac cgtaactgtg
     4801 acagtgtaga ctgtcaaggt ctgtctcgcg ccttcccgaa aggtcctgat atccttggga
     4861 tggaacgtgg cgacccaggg tgaaacatgc atgcgtccgc tggtgctcta tgcagatgac
     4921 gaagcgaaca accgcatcgt cttcgcacaa tcgttcggca accagttcga tgtgatcacc
     4981 gttcccgacg gagccgcggc actcgcgctg ctagaaaccc gcgaagccgc cgtcctcgtg
     5041 accgacatgc gaatggaagg gctttccggc gaggagctcc tgcggatcgc caaggatcga
     5101 catccgcgga cgatccgcat cgtcgtcacc gcgtactccg atatcgatcg gatcttgcgt
     5161 gcgctcaacg aagggctggt tgcccgctac atcatcaagc cgtgggagca cgccgaaatc
     5221 acgcaggtgc tgcgctgggc gtgcgaagca tggtcgctgt cgacgaatgc tgcagcgctc
     5281 cacaaccggt tggccgagac cgaacgcctt gtcgcctcgg cgagctcgtt cgttcacgat
     5341 gtgaagtcac cgatcatgtc gctcctcgcg aacacggatc acctgcacga gcttgcgggc
     5401 gatgtacctg cgttgcgagc cgcgctcgct gcggtaccgc tggcggacga acatcggcgt
     5461 gcgctcgcat tcatcctcga cgagctgctg ccgatctcgg aggaaatgca gacatcgacg
     5521 cggcggatcc aggaattggt cgaaaccgtc gccgcgaggc cagctccggc accgatcatc
     5581 gatccgttgc agatcgtgcg tcacgtgatg gcggtatgcc agccgctcgc cgcgcagctc
     5641 agcgcgtcgc tcgactacga aggtccgggc gcgttgcctc ggatccgcat gtcggcgagc
     5701 gagctcagcg aggtcttgat caacctcgtc gcgaacggtg cgcaggccgt cggtgcgcgt
     5761 ggaacgacgg ggcacgtgtc gatcactgct cacttgctgg acgggatgct cgagctccag
     5821 gtgcgcgacg atggcgtggg aatcccggcg gcagcgatga gtcgcgtggg cacgccgttc
     5881 tatacgactc gaatcggcgg catggggctc ggcgtcgctc agtgccagcg actcgtagcc
     5941 atcgcaggcg gccggttctc gatcgatagc caacccggtg ttggcaccat cgcaacgatc
     6001 tcgattccca ctgcagcgta actattgccg cgaccctgcc gcgaaacgct gcagggcaga
     6061 cgctctcgag tgcgttatcg tggtcgaggt tgatgaagcg catcctgttg atggacgaca
     6121 gcgagatgtt tctcgaggtc acgtgtacgg cgctcaaagc cgtcggctac gacgtgatct
     6181 gcgcgaatga cctcgcgcaa cttgccgagg ctcgcaagca aggcccgtcg gatctcgtgc
     6241 tgatggatgt gcagatgccc gaggcattcg gcgacgatat cgcgctcacg ctgcggcacg
     6301 tgtacggcat ctcggcgccg atctacttgc tgtctagcct tgccgaaaca gatctcgcgg
     6361 accgcgtcga atgggcaggc atcgacggct tcatttcgaa gcaactcggt gtcgaagcca
     6421 tcatcagccg cgtccgctcc atcgtaccgg tcgaatcgtg agcgagcacg acgacgacga
     6481 cggtagcgag cagcgcaagc acgcacgcgt gccgctccgc ttgatcgtcg agtacgagga
     6541 tgctgacgat tttgttggcg actacaccga gaatctctcg gccggcggca ccttcattca
     6601 tacgacgcgc ttgctcgaac gcgacaccgc agttcagctc gtgctgtcgt ttccagggct
     6661 attgcaaccg atcgccctcg aaggcatcgt gcgctggtcg cgaggcggca agcagcccgg
     6721 tgtcgggctc gaattcgtgc cgaaccagga ctacgcgaag ctcgagacgc tggttcgcct
     6781 ggtcgaggca cgtgatccgc gtgcggtcgc ccgcgtcgtt cgcgtgctgc tcgccgagga
     6841 caatcctcac atcgcggagc tgatctgcaa cggtgtagga gcatctgcca agcgcacgtt
     6901 tggcgacgcg ctcgcgttct actttgcgac tgccgagaat ggcgccgttg cggtcgagtt
     6961 actgaaaacc gcgccgttcg atgtcgcgat catcgatctg tacttgcccg tactggacgg
     7021 cgccaaggtg atcgagaacg cacggtctga cctcggcctc acgaagctgc cgatcatcgc
     7081 gatctcggcg gcaggcgaca tggcgaaacg ctcggcgctc gatgcgggcg caaacgtgta
     7141 tctgagcaag ccgatgcgac tgcgtgaagt gctcgagtcg atgcgccgac tcgtcccgat
     7201 gagtgcgtga gcgcgggagt gaaaccggcc gtgttatctt gaggtgtcga tggcgcaacg
     7261 caagatcctg ctgatcgacg acagcgaggt gtcgcttcac ttcgcgtcgc gcattctggg
     7321 gaaagccggc ttcgatgtcc ggaccacact cgacgtccac gatctgcaga gcgtgctcgg
     7381 cggttggcgc cccgacatca tcctgacgga cgtggacatg cccggtgtca ccggcccgga
     7441 gctgtgccgg atgctgaagt cgagctacga gaccgcgcac gtgccggtcg tcttgttctc
     7501 ggcggttcct tcgcgagagc tcgagacgat ggcgcgcgag tgcgaagcgg atggcttctt
     7561 gtcgaaggcg aacgggctcg agcggctgcc gcaagagctc gagctgttga tcgaaacggc
     7621 gttgttctag cgttggggct aggcgaccgt gtttgcggca ggaaagcgca cgcagatcga
     7681 cgtccctggc ccgggcagtt gttcgatctg accatcgagc tgtcgcgcaa gcgtcacgat
     7741 gatctgcaat ccgaatgtct tcggggtcgc gagattcagc ggacccgata tgccgacgcc
     7801 gtcgtccgtt acggtgattg caagcgacgc ttcctgctgc gcgaagcgca catgaaccgt
     7861 tccggcgcgt ccgcccggga acgcgtgcgt cagcgcgttc gtgacgagct cgttgacgag
     7921 catgccgcac agaatggcgc ggtcgatatc gagctgaacg tgctctacat cgacatccgt
     7981 ggaaacgtgt tcggcgacct cgccataagc ggccgctagt cgctggatga gctcctggag
     8041 gtattcgccg aagtcgacgt gtgcaacctc catcgactgg tacagcaact gatggactag
     8101 cgccatcgat tggatccgac tctgattgtc aacgagacgg cggcgcgtcc gttcgctcgg
     8161 ttccgcgatg atctggagat tgagcaagct cgacatgacc tgcaggttgt tcttgacgcg
     8221 gtgatggagc tcgcgcagca agaggtcctt tgcgcgcagc gagctctgca gctctgcggt
     8281 gcgcgtcgcg accgtgcgct cgagcttttc gttcgcctct tgtcgcgcgg cttcggcagc
     8341 ttgttccgcg ctgatgtcgc gaacgaatcc cgtgaggacg ggctcgtcgc ccgtcgatgc
     8401 gacggtgacg gcgacctcga ccgggaactc atgagaatcc tgcgcacgcc acccgatggt
     8461 tcgatacctg gagccgatca cgggaccgct accttcgcgg tagtacttct gcagcccctg
     8521 atagtaggca gcgcgtagcc gcggaggaat gatgacggcc gctaggtcgt tgccgaggac
     8581 ctccagcttg cggatcgaga acagctgttc ggcagcagga ttgacctcca gaatgcgtcc
     8641 gtgcacgtcg atcgtaaaga tcgcgtcgag cgcagtctgt tggatcgcgg cggtgcgttg
     8701 tacagctcgt acgcgttcct gcgcgagcgc gctgagcatc aacgtcgtga ccgaagagag
     8761 cgcgacgaag atctggaggt ctaactgggc agcgaacggt gcgacgccca gtgcggcggc
     8821 gacgattgcg gataggctca cgacgaaact tgcggtcgct gcgccgcggg gcccgaagcg
     8881 aacgccagcc cacaacagca acggcagcaa gtagtacgtt cgtgacgccc tgagccagga
     8941 cggcagccaa ccggtcagga ccagcagaac gagcagcagg ccgagggcaa cgaacgcgat
     9001 cgtctcgagc cggcgccatc ggccctcgaa cgtcttggga agcgaggtcg gcagcgccca
     9061 cgtgaacaag acaggacaca cgatcaggtc accgctgaga ttgatccacc accacgtcga
     9121 ccacaggttg acgaagtcag cgtggccatt aaatccggtc gcgagcagtg cggccgcacc
     9181 gaacgccgca tcagcggccg aaatcgtcgc atcgacggct gcaaacgaca cgacgtcgcg
     9241 cacgcgttcg agctgaggcc ggaagtggag cagcttgcgc agcacgtagt agccgaccat
     9301 cgcctcgaga acctcggtcg tcgcgacgat gaaggcgacc tcgggaggat tccggatgat
     9361 taccgacgca aacgctccca agaagagtcc aggccaccag cggagcccgt acagcagaac
     9421 caacgcaaac ccgatccccg gcggtgccca gatcagcgag tacacgccag cgagcttgtg
     9481 aacgcgcaat gcgagctcgc cgacggcgaa gtaggcgatg gcgatcgcga cgacccgcaa
     9541 cgcgtagccc gccctgcgac gactatcggc agcgagtcgc acccacgggg tctacctccg
     9601 gagagccaac ggcgcgtgac cgccgtcaag cgcgctgacg gcacatgggt cgctggcgct
     9661 cgacgcgcct actggttcac gcttcgatca cgtgccacgc ggtacacgcg ccgtaggcgc
     9721 tacgcctggc gattccaggt cacgcggtag agacacgcgg catcgccgcg cgagatgcag
     9781 gcttcgtgat cgacgatcgg cccgcggccc cccgagagca cgatgagctg ttcgagcaga
     9841 ccatcggtcc acgcgcacag ctccgcatcg gggagcgcgt ccgcgatgcg aacaacggtc
     9901 tccgacgggt tgaccggcat gctcgtcacc acgccccagc tctgatactg gccccagaca
     9961 tttcggatcg ccgacaacgt gcgttcgggc acgagcgttg cggagcttgc cggaaagaag
    10021 cgcctgaacg atgcacgcac ggtcgcgcgg gcgatgtcgc gcgcgagccg tggaccatcc
    10081 cgatcggtgt gttgcggcgc gatcgcgagc aaccggctga acagctcggt cggcatccac
    10141 gcgagcggtg cggtgtcggt cagcgcggcg gcgaggtcgg ggtgagcacc accgatcgta
    10201 tcgcggagct tctgtgcttc acgaacaccg agcgcgcgcg cgaccgagcg gaacacgacg
    10261 ccgcgagtcc tcgcgatcgc acgccgcggt agcacgacgt tgccgttcca ccgcggaccg
    10321 tcgggcttgc gcgcgagggt ctcgatcgcg agcgacgcgc gcaacgaggc cgcgaatgcg
    10381 ctcgcagatg ccggacggcg cgccggatcg ccagcaaggg cgtctgcgaa cagcgcatcg
    10441 atctcggcaa gctccgcgcg atgcgacgac ggcggcggca cgggttcacc gctgatctgc
    10501 cgcgcgatca cgtcgtcgcc atctccccag ggatgggcaa gtgtgaggag tgcgtaggcc
    10561 gtcgccgcga gcccgtatac atccgagcgc accgtggctt cttggccggc aatcacttcg
    10621 ggagcgacat agcccggtgt accggctgct tcgacgaact ggccaaaccg gcgcgcgatt
    10681 ccgac
//
LOCUS       DASUFX010000488         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_911666,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000488 DASUFX010000000
VERSION     DASUFX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911666"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFV99_19650"
     CDS             <1..1483
                     /locus_tag="VFV99_19650"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5061597.1"
                     /translation="TAQAITVLTPPARAVAAKRVLFVIDGSRSMELIGRHRVKQVVHA
                     LANVLTRGTEIEAIVYDRSAQRVLGGWQAIDAKQVTAIETMIDAHTAGNGSDAKSALV
                     VAQQAIGGTRGETQIVLITDGVMGEMTDNALGDLIAPLQVELHAIVLSHARMRIPDTL
                     PLRAAINRVGGSYTELDVDELDSALTMLDDWLRPSWLELQLSTPPTLVRTAPRAFATA
                     AFNIPDALHAGAGVVVTTIEKRPRKPVLTGHAAKPIKISSMTGPAAPIAELALAHIEP
                     DALFEELPKLRARHPVVNDDHPFVVLATQGKIAKNRREMTASGGPYTRMVAVDDPQFP
                     PERRPQAAQVGGSAIDRVSLELMFKTQLQPAAFACYQRALGRLPKLAGTAEFHLEIGR
                     GETTRATVTGLGDATFDACLLDAAYTVTPSLPNPTYNSDDRTVANYPLSFSVREQKPF
                     IIAGDADSSSPLDIDKIQGGVPVHIKAGDTSTPLGDLRVPKSP"
     gene            complement(1711..2340)
                     /locus_tag="VFV99_19655"
     CDS             complement(1711..2340)
                     /locus_tag="VFV99_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061598.1"
                     /translation="MRFPALLMLAALAGCPSKKKQETVEQKPVVGELVRGDAGITAVV
                     TGVGPGEMHVDEDGAPHAVQTQPQPNRPGRPIDVTLRSTPPGAQVAVDGTVIGNTPSY
                     WNGMADGREHEFVFTMRGHAIARYRFVPVSSGVIHGRLDPIHEETDAGVPAPPPEVMA
                     PKPTGAVTPPNPPTTVVQDAADVPPGPVPGPVGPTPSAGSSDTPVGPQP"
     gene            complement(2363..>2556)
                     /locus_tag="VFV99_19660"
     CDS             complement(2363..>2556)
                     /locus_tag="VFV99_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061599.1"
                     /translation="EALRQQLENPSANENDVSRAIDNVSRLEADIRKARIIAWVKARR
                     VLDDGQRQKVERSPRRKSR"
BASE COUNT          410 a          903 c          838 g          405 t
ORIGIN      
        1 caccgcgcag gcgatcaccg tgctcacgcc gccggcgcgc gccgtcgcag ccaagcgcgt
       61 gctgtttgtc atcgatggct cgcgcagcat ggagctgatc ggccgccatc gcgtgaagca
      121 ggtcgttcac gcgctcgcga acgtgctcac gcgcggcacc gagatcgaag cgatcgtcta
      181 cgaccgcagc gcgcaacgcg tgctcggcgg gtggcaagcg atcgacgcga agcaggtcac
      241 cgcgatcgag accatgatcg acgcgcatac cgccggcaat ggcagcgacg ccaagagcgc
      301 gctcgtggtc gcacaacagg cgatcggcgg cacgcgcggc gagacgcaga tcgtcctgat
      361 caccgacggc gtgatgggcg agatgacgga caatgcgctc ggcgatctca tcgcgccgct
      421 gcaagtcgag ctgcacgcga tcgtgctctc gcacgcacgc atgcgcatcc ccgatacgct
      481 gccgctgcgc gccgcgatca accgcgtcgg cggcagctac accgagctcg atgtcgacga
      541 gctcgacagc gcactcacga tgctcgatga ctggctcagg ccgtcgtggc tcgagctcca
      601 gctcagcacg ccgcctacgc tggtccgcac cgcgccaagg gcgttcgcga ccgcagcatt
      661 caacatcccc gatgcgctgc acgctggcgc cggtgtcgtc gtcacgacga tcgagaagcg
      721 gccgcgcaag ccggtgctga ccgggcacgc cgcaaagccg atcaagatct cgtcgatgac
      781 gggtcccgca gcgccgatcg ccgagctcgc gctcgcccat atcgaacccg acgcgctctt
      841 cgaagagctt cccaagctgc gtgcacgcca tcctgtcgtc aacgacgacc atccattcgt
      901 cgtgctcgcg acccagggca agatcgcgaa gaaccggcgc gagatgaccg cgagcggcgg
      961 tccgtacacg cgcatggttg cggtcgacga tccgcaattc ccgccggaac gccgtccgca
     1021 agccgcgcag gtcggcggca gcgcgatcga tcgcgtctcg ctcgagctga tgttcaagac
     1081 gcagctgcag ccagcggcat tcgcgtgtta ccagcgcgcg ctcggacggc tgcccaagct
     1141 cgccggcacc gcggagttcc acctcgagat cggccgtggc gagaccacgc gtgccaccgt
     1201 gaccggtctc ggcgatgcga cgttcgacgc gtgcttgctc gacgccgcat acacggtgac
     1261 accgtcgctg ccgaacccga cgtacaacag cgacgaccgc acggtcgcga actatccgct
     1321 ctcgttttcg gtgcgcgagc agaagccgtt catcattgcg ggcgacgcgg actcgtcgag
     1381 cccgctcgac atcgacaaga tccagggcgg cgttcccgtc cacatcaagg ccggcgatac
     1441 ctcgacgcca ctcggcgatc tgcgtgtgcc gaagtcgcct tgagagttgg tgacgatgcg
     1501 ggcgagtagt accttgttac tggcatgcca ccgccgttga aacccgagca cgccgaacga
     1561 cgactgttcg tcgaggctgc gctcgctgct cgctccgatt acgctcgaac gggctcggtg
     1621 tcgacgctgg cgaactgcac gcgtatctga aagcgcgagc ctccggtcgc cgtgctgcac
     1681 gtcccagcgc gcggcggtgg cgcgactaac ctagggctgc ggaccgaccg gcgtgtcgct
     1741 cgagccagcg ctcggcgtcg gaccgaccgg acccggcacg gggcccggcg gaacatcggc
     1801 ggcgtcttgc acgactgtcg tcggcggatt cggcggcgtc accgcgccgg tcggtttggg
     1861 cgccatcact tcgggcggcg gcgccggcac accggcatcg gtttcttcgt ggatcggatc
     1921 gaggcggccg tgaatcacgc cgctcgacac cggcacgaag cggtagcgcg cgattgcatg
     1981 gccacgcatc gtgaacacga actcgtgctc gcggccgtcg gccatgccgt tccagtacga
     2041 cggcgtgttg ccgatcacgg tgccgtcgac cgcgacctgc gcgcccggcg gcgtcgaccg
     2101 caacgtcaca tcgatcggcc ggcccggccg attcggttgc ggctgcgtct gcacggcgtg
     2161 cggcgcaccg tcctcgtcga cgtgcatctc gcccgggccg acacccgtga ccactgcggt
     2221 gatcccggcg tccccgcgca ccagctcgcc gacgaccggc ttctgctcga cggtctcctg
     2281 cttcttcttg ctcggacagc cggcgagggc cgcgagcatc agcagagccg ggaaccgcat
     2341 gggtcccaag ctatagcatg cgctagcggc tcttacggcg tggcgagcgc tcgacctttt
     2401 ggcgctggcc gtcgtcgagt acccgacgcg ctttcaccca ggcgatgatg cgtgccttgc
     2461 ggatgtctgc ttcgagtcgc gacacgttgt cgatcgcgcg cgagacatcg ttttcgttcg
     2521 cgcttgggtt ctcgagctgc tggcgcaggg cctccg
//
LOCUS       DASUFX010000489         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_356036,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000489 DASUFX010000000
VERSION     DASUFX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356036"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="VFV99_19665"
     CDS             <1..1794
                     /locus_tag="VFV99_19665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5061600.1"
                     /translation="EVDIAMRDGVKLHTVILVPKGAKRAPMLLTRTPYDADKMTAKSD
                     SAHLAMTLDGYDHPVETVIEGGYIRVVQDVRGKHGSEGDYVMNRPIQGSQNPTTVDHA
                     TDTYDTIDWLVKNVPESNGKVGILGISYDGFTSAIALVNPHPALKVAVPMNPMIDGWR
                     GDDWFHNGAFRQVGLDYIYEQEATRKNDEDWPTTSFDQYDTFLDAGSAGELARRRGMD
                     QIGFWQKLVAHPSYDAFWRDQAVDRVLAKLPVPIPTMIVHGLWDQEDIYGSAALYKAL
                     EPKDKANDKVFLVIGPWRHGQQIGDGNALGPIRFDSDTSKTFRREILLPFLDQYLKDK
                     APKADIAPVMAFETGTNKWRRLTAWPSGCAKGCSIKPTPLYLGASSQLSFAAPKADGY
                     SEYDSDPAKPVPYIPRPVAPGSYESNKAWREWLTTDQRNAASRPDVLVFTTDPLTSPV
                     KISGEPIANLVAATTGTDADWVVKLIDVYPPEVPAHVELGGYQLAVAMDIFRGRYRES
                     LETPKAIAANQPLLYKFALPTTNHVFLPGHRIMVQVQSTWFPLYDRNPQTFVPNIMLA
                     KPSDYRKATQRIYHSPKQASFIELPIVNGSR"
     gene            complement(1825..>2142)
                     /locus_tag="VFV99_19670"
     CDS             complement(1825..>2142)
                     /locus_tag="VFV99_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061601.1"
                     /translation="EVVRVVGEKVVVTAPTDDLGQFRVTGLAAGPYRVEVERGTVRPP
                     LPPPRPPIVITRNELRCCVCAMERARSFGTVLGRAAQDPAKLQPIDTRSTTQGVVIRD
                     PGR"
BASE COUNT          421 a          735 c          664 g          322 t
ORIGIN      
        1 gaagtcgaca tcgcgatgcg cgacggcgtg aagctccaca ccgtcatcct cgtaccgaaa
       61 ggcgcgaagc gcgcgccgat gctgctcacg cgtacgccgt acgacgccga caagatgacg
      121 gcgaagtcag acagcgcgca cctcgcgatg acactcgacg gctacgacca tccggtcgag
      181 acggtcatcg agggcggcta catccgcgtc gtccaggacg tgcgcggaaa gcacggctcc
      241 gaaggcgact acgtgatgaa tcgcccgatc cagggctcgc agaacccgac gaccgtcgat
      301 cacgcgaccg acacctacga cacgatcgac tggctcgtga agaacgtgcc cgagtcgaac
      361 ggcaaggtcg gcatcctcgg catctcgtac gacggcttca catcggcgat cgcgctcgtc
      421 aacccgcatc cggcgctcaa ggtcgcggtg ccgatgaacc cgatgatcga cggctggcgt
      481 ggcgacgact ggtttcacaa cggcgcgttt cgccaggtcg gcctcgacta catctacgaa
      541 caagaagcga cgcgcaagaa cgacgaggac tggccgacca ccagcttcga tcaatacgac
      601 acgttcctcg acgcaggctc cgcaggcgag ctcgcgcgcc ggcgcggcat ggatcagatc
      661 ggcttctggc agaagctcgt cgcgcatccc agctacgacg cgttctggcg tgaccaagcg
      721 gtcgatcgcg tgctcgcgaa gctgccggtc ccgatcccga cgatgatcgt gcacggcctc
      781 tgggatcaag aagacatcta cggcagcgcc gcgctctaca aagcactcga gccgaaagac
      841 aaagccaacg acaaggtgtt cctcgtcatc ggcccgtggc gccacggcca gcagatcggc
      901 gacggcaacg cgctcggacc gatccgcttc gacagcgaca cgtcgaagac attccgccgc
      961 gagatcctgc tgccgttcct cgaccagtac ctcaaggaca aggcgccgaa ggccgacatc
     1021 gcgccggtga tggcattcga gaccggcacg aacaaatggc gccgcctgac cgcgtggcca
     1081 tcgggctgcg cgaagggctg ctcgatcaag ccgacgccgc tctacctcgg cgcgagctcg
     1141 cagctgtcgt tcgccgcgcc gaaagccgat ggctacagcg agtacgactc cgatccggcg
     1201 aagccggtgc cgtacattcc gcgccccgtc gcgccgggta gctacgagag caacaaggcg
     1261 tggcgcgagt ggctcaccac tgatcagcgc aacgcggcgt cgcggcccga cgtgctcgtg
     1321 ttcacgaccg atccgctgac gtcaccggtg aagatcagcg gcgagccgat cgcgaatctc
     1381 gtcgcggcga cgaccggcac cgacgcggac tgggtcgtga agctcatcga cgtctacccg
     1441 cccgaagtgc ccgctcacgt cgagctcggc ggttatcagc tcgcggtcgc aatggatatc
     1501 ttccgcggtc gctaccgcga gagcctcgag acgccgaaag caatcgcggc gaaccagccg
     1561 ctgctctaca agttcgcgct gccgacgacg aaccacgtgt tcttgccagg ccaccgcatc
     1621 atggtgcagg tgcagtcgac gtggtttccg ctctacgacc gcaatccgca gacattcgtg
     1681 ccgaacatca tgctcgcgaa gccgagcgac tatcgcaaag cgactcagcg catctatcac
     1741 tcgccgaagc aggcgagctt catcgagctg ccgatcgtga acggcagccg ctgagctagc
     1801 tcgcgatcgt ccaatcgctc caaactaccg gccggggtcg cgaatgacca cgccttgtgt
     1861 cgtcgaccgc gtatcgatgg gttggagctt cgccggatcc tgtgcggcgc gccccagcac
     1921 cgtgccgaag ctgcgcgcgc gttccatcgc gcaaacgcag catcggagct cgttgcgagt
     1981 gatcacgatc ggaggtcgcg gcggcggaag tggcggtcgc acggttccgc gctctacctc
     2041 gacccgatac ggcccggccg cgagtccggt cacgcggaac tgtccgaggt catccgtggg
     2101 cgcggtgacg acgaccttct cgccgacgac gcggactacc tc
//
LOCUS       DASUFX010000490        13345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_217974,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000490 DASUFX010000000
VERSION     DASUFX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217974"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFV99_19675"
     CDS             complement(<1..525)
                     /locus_tag="VFV99_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061602.1"
                     /translation="MDDLLRALSTASPEDLAAWRRLIDRAGPASVASWLAPKALIACM
                     LGDTRFAAGALETLRAEGSAAAQQVAAAFPEAAALAHPAPPQIEHDPPRLEAQGSRQA
                     QPRPLLDHIATRLLGRKLAGLEARDLATFQDSSKPGLSQQAFDELATVTKRVLDAGLG
                     PALRAAVIHLDIAKT"
     gene            632..1153
                     /locus_tag="VFV99_19680"
     CDS             632..1153
                     /locus_tag="VFV99_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061603.1"
                     /translation="MIARCGLIVAVIASCSGEPPERLHVPAKLIELAPLPPPPPPPPP
                     PPPLDTTVEMPDELSDVISPYGTPPAVTIVELTEGNAHGVFLGYGILRTKPLTDMHAK
                     QLVDKLAADNAFIDGGDYGCVGDPIGLRFARGSILRDVVVDCGHMYLTPSRHDGRFAL
                     LAPDVNQFIYALR"
     gene            1203..1730
                     /locus_tag="VFV99_19685"
     CDS             1203..1730
                     /locus_tag="VFV99_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061604.1"
                     /translation="MVVGCASARPEPLDTPAQRVREPGPVAPVRKPRLLMPILTVVDA
                     TTPVEPPAIRIDKRAGIMGRGGGGFSVWYDGTVQFRGPYCAEPRRGTVEAARVGELMA
                     TLGRSGLFERKQRPIDRGCGYALDGSYWDIALRAAERSNTFTGPDTAPQRTCAWVEDM
                     LDAIGTVIGANPCGP"
     gene            complement(1738..3144)
                     /locus_tag="VFV99_19690"
     CDS             complement(1738..3144)
                     /locus_tag="VFV99_19690"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5061605.1"
                     /translation="MTIVSVLERELGSDAIIGPGHEKLDDYGRDESPLPEFFPPECAV
                     LCSSTEQVAAVLRICAERKVPVTPRGAGSGMVGGALPLQGGIVLSTERMQAIKEIDPD
                     DLVCVVEPGVVTGRLQEQVEAQELFYPPDPASLEFCSIGGNAATNAGGPRAFKYGVTR
                     EYVLGLEVGLIGGEVMRCGRRTAKGVTGYDLVAGFVGTEGTFGVITELTLKLLPRPPA
                     AATVLGVFADVPKAGAAISSLLRGGLRPRVLELADKFSIDCVRAKTRYRFPDNAGAIV
                     LIEVDGTPDSMAAQMEAIGLACDELGALDVLGATEPAERRAVWEARRMISPALKEAWR
                     IKVNEDVCVPRGKIVEMLARVDRVSQETGIPIAVFGHAGDGNLHVNLLSNDDPQDPAV
                     RKRMWAAAERVFAHTVELGGTLSGEHGIGLTKRDYLHMEQSEQVLAWQRKWKSLWDPT
                     GLLNPGKVIPGRKTACTE"
     gene            complement(3146..3529)
                     /gene="erpA"
                     /locus_tag="VFV99_19695"
     CDS             complement(3146..3529)
                     /gene="erpA"
                     /locus_tag="VFV99_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5061606.1"
                     /translation="MSDAAVASMSDSLDPQGPESMLRITPTAASKVNEIRQAEGIEGN
                     MALRLRVVGGGCAGFSYDLYFDEPAEVDRQFEVEGVKCIVDEMSLMYLVGTEIDYVEG
                     LQGAGFKFQNPNVKSTCGCGSSFSV"
     gene            3587..4750
                     /gene="moeB"
                     /locus_tag="VFV99_19700"
     CDS             3587..4750
                     /gene="moeB"
                     /locus_tag="VFV99_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX5061607.1"
                     /translation="MPSYDALLRELRSQTPEIDVQGLSDQLGNVALIDCRESDEYSQG
                     MIPGTVWIPRGKLEQKIEKAVTDRSAPIVIYCASGNRSLFAQRTLVELGYTNVKSLAG
                     GFTAWKRAGLPWDMPATLRPDQETRYSRHTLLPEVGVAGQQKLLAGKVVCIGAGGLGS
                     PSSMYLAAAGVGTLGMIDDDIVDASNLQRQILHGTDRLATPKVESAERTLKNLNPDVR
                     VEKYRARITSANAIDLLGRYDVIIDGADNFATRYLVNDVALKLGKPVIHASIFRFEGQ
                     ITVFPANGSPCYRCLYPQPPPPEDAPSCAEGGVLGVLPGIMGVLQATEAIKLLLGLGD
                     SLAGRLLVYDALRTRFRELKLRRDPKCPTCGDGVDRTAIELVDYEQFCAGPKH"
     gene            4769..6604
                     /locus_tag="VFV99_19705"
     CDS             4769..6604
                     /locus_tag="VFV99_19705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061608.1"
                     /translation="MIAAGQSIGSYRILQKIGTGGMGAVYLAEHPLIGKKVALKVIHR
                     ELASNKEVVQRFFQEAKAVTKIGNEHIVEIHDFGQTPEGDHFYIMEYLEGQTLATVLA
                     TDRVLDVMRALHIGAQVAAALAAAHAAGVIHRDLKPDNVMLTTRLGDNNFVVLLDFGL
                     AKMFVGGGQSAVKTAAGVLLGTPQYMSPEACESKGNLDHRTDIYALGILLFQMLTGML
                     PFDGETMGEVLVKQVTQLPPPPRGYNQNIPPAVEQIVLRCLAKSVDARFPTMLALREA
                     LLDPEAYLRKSPPMSPARSLAPGEAKVDAKTVMAYAAQEQRTKIDMGGVAPSRGTGPQ
                     NAMPLPAPAPSLPGNAGAKTMIASPGMPLPQAPAPPPQAPEMVAPAQPKMNTMRIATP
                     LGYSSRPPRKMWPIVLVFALLLGLGGGAFAVAWFGKTDGDGGGGSASGGGSASGGSAT
                     ASNPLPPAPKPDAQVVVENKGSAAPQNAGSATPVKVDKVPAGSGAGAAAGSAAPGTPN
                     NAQKPGMAVIVIDSTPQGAEVLGPDKKSLGKTPVTVSLPISDMPQDFELKLAGYKKKT
                     KQIMITGNSVINVMLDRAPSSGRSGKGSGKKKGSGDSELMRPDDL"
     gene            6626..8203
                     /locus_tag="VFV99_19710"
     CDS             6626..8203
                     /locus_tag="VFV99_19710"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEX5061609.1"
                     /translation="MVFAVVAGLVVLAVAAFFAARVSAAEQLRRVSRAIDEARMQAAA
                     ERKAAELAAAAQRTKAEAAARNEALEASAKVDEELAEREAKLAEREDALHVRRSELDH
                     FEDSVEAREGQLGQIQRDVQSRHDRARGLDQDVQRKRETMKGELENRAGIKAQELIRQ
                     MSAVWIEEARARAAALVRNAEQSTADPQHDRDARRVMEIAAARCSMHFLTERNLSTLR
                     VGPDIVSILLDNNAAIHGALERVAGVQLQINDEKDVVRLEGLDGVGREVVRRALSRMI
                     KKPETIDAARKAPDEWAAKAREHLEQEIRALGKKAFSTLGIQKAHPEIVDLVGALNFR
                     TSYTQNQWLHAVEASFLAGMMAAELGLDEKLARRATLMHDIGKALTHKIEGSHAVIGA
                     DIARRLGESELVANAIGSHHADEPPNSVYAYLVAASDAMSGARPGARREQAEGFSTKL
                     EDLERIGLQRRGVSHAHAVHGGRELRVYVREKEVDDLTVVEMSTEIANQIAEEMTFPG
                     QIKVTVIRAFEATSTAN"
     gene            8341..9018
                     /locus_tag="VFV99_19715"
     CDS             8341..9018
                     /locus_tag="VFV99_19715"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5061610.1"
                     /translation="MANHGNGRHVIDDDDDDRHTSRDDRYARGREEGRYVTRDEPRNV
                     PPSQRYGHWEDRTTRTERDEGYRKLQSRELAYPGGFEQRARERDDDDEPDDTRGGYLG
                     QGGQQLGYVESRHGHHQEGGMYGHGPGTWGERRTSHRGKGPANYTRSDERIRELVCEA
                     LTDDHDVDATRIEVTVTDGEVSLAGTVEDRYQKRMAENCAECVSGVKDVHNQLRIAVD
                     KQRPDRA"
     gene            9325..9540
                     /locus_tag="VFV99_19720"
     CDS             9325..9540
                     /locus_tag="VFV99_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061611.1"
                     /translation="MLMLLIARVDFFASALAWQVRGEVAGSALHCPHRVVHPVVRARP
                     QMRTLGMHHRYMMRDDVGRRRLLQLDQ"
     gene            complement(9541..10455)
                     /locus_tag="VFV99_19725"
     CDS             complement(9541..10455)
                     /locus_tag="VFV99_19725"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5061612.1"
                     /translation="MSARNKLVVTTPSDLEIEMTRDFDAPRALVWEMFTKPEHVRQWW
                     GTGHVEMTVCEIDFRVGGKYRYVGKTPDGREVPFCGEHHEIVYPEKVVYTEIFDVDMA
                     REHPSLITTTFTENNGKTTMRLVAKYDSKQTRDIVLQSGMEKGAAKGYDKIEEIVGGL
                     AQLTIEREFTAPRALVWKAWTETTQFAKWFGPHGMVVPRVEFDLRPGGALRYDMRREG
                     MAMTIVGAFTEVIDGERLAYTERSEHDGKLMSEHLNTVTFGERDGKTVLTLTMTVLVS
                     GMDPKRYIGGAGAGWNQALDKLGELLAR"
     gene            complement(10439..10792)
                     /locus_tag="VFV99_19730"
     CDS             complement(10439..10792)
                     /locus_tag="VFV99_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5061613.1"
                     /translation="MSKNLDTTFAALADPTRRAILARLAEGQDGVSVTELAAPFEMSL
                     PAISKHLKVLERAGLIIRGRDAQWRPCRLDARPLQEVSVYLEKYRQFWEASFDRLDDY
                     LKTIQQKDKKDERKK"
     gene            10946..11509
                     /locus_tag="VFV99_19735"
     CDS             10946..11509
                     /locus_tag="VFV99_19735"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5061614.1"
                     /translation="MDESNPRHRSAADKLMRVGELAAAVNKTVRAMHLYEELGLLEPR
                     ARSEGGFRLYGPEAIDRIHWIVKLQAIGFTLAEIQGFVKDFQSAGSAPEATARVRALF
                     QEKQQNIREQITQLSVIENDINEALAYLDSCQTCSTDYAPTECGACDHQGHQKGEAPP
                     LFAGISKRHEEKEFDVPVTVLYREGNN"
     gene            11509..12741
                     /locus_tag="VFV99_19740"
     CDS             11509..12741
                     /locus_tag="VFV99_19740"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX5061615.1"
                     /translation="MSEVKTPIFMDNHSTTRVDPRVLETMLPYFDVHYGNAASRNHVF
                     GWTAEAAVENARNQVASLIGGDGKEIVWTSGATESNNLALKGAASFYKKQGNHIITVE
                     TEHKSVIDTCKRLQREGYEVTYLTPQKDGLVTPEMVTAAMTDKTLVVSIMLANNEIGV
                     VQDVNALGAAIKAKNPKTLFHIDGVQGAGKIPFDVNAAKADLVALSAHKMYGPKGVGA
                     LWVRRKPRVRIDPIIDGGGHERGMRSGTLAVPLIVGFGKAAALAKEEMQAEAIRLKAL
                     RERLYRGITAKLPETYVNGSLEDGRRLPGNLNISFAFVEGESLLMALKDVAVSSGSAC
                     TSASLEPSYVLRALGVGDELAHTSIRFGLGRFNTEAEVDYVIDLVVKSVDRLRQLSPL
                     WEMHQEGIDINSIQWTPH"
     gene            12752..13186
                     /gene="iscU"
                     /locus_tag="VFV99_19745"
     CDS             12752..13186
                     /gene="iscU"
                     /locus_tag="VFV99_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEX5061616.1"
                     /translation="MAYSDKVLDHYENPRNVGTFAKDEAEDVGTGLVGAPACGDVMRL
                     QIKVKDGRIEDAKFKTFGCGSAIASSSLATEMIKGMTLDQAASITNTQIVEELNLPPV
                     KIHCSVLAEDAIKAAIADFKKKKASREVKAETPAAEARRQEG"
BASE COUNT         2424 a         4359 c         4409 g         2153 t
ORIGIN      
        1 cgtcttcgcg atgtcgaggt ggatgaccgc ggcgcgcagg gcagggccga ggcctgcatc
       61 gagcacgcgc ttcgtgacgg tcgcgagctc gtcgaacgcc tgttgcgaca ggcctggctt
      121 gctgctgtcc tggaacgtcg cgaggtcgcg cgcttcgagg ccggcaagct ttctgccgag
      181 gagtcgcgtc gcgatgtgat cgaggagcgg gcgcggctgt gcctgccgcg agccctgcgc
      241 ttcgaggcgc ggcggatcgt gttcgatctg cggcggagct gggtgcgcga gtgcggccgc
      301 ttcgggaaac gcggcagcca cttgctgcgc agcggccgac ccttcggcgc gcagcgtctc
      361 gagcgcgccg gcggcaaatc gcgtgtcacc gagcatgcat gcgatcagcg ctttcggtgc
      421 gagccaactc gcgacgctcg cgggaccggc ccggtcgatg aggcgccgcc atgccgcgag
      481 atcctcgggt gacgcagtgg atagcgctcg caacagatcg tccaccgaac caatctacac
      541 gtgccgcacc tgcgcggtcg acgacgcgac gccacatccg gtgtcagcgc gcatcgcatc
      601 agcatctcga tgcaggagct agccttgaat cgtgatcgcg cgctgtgggt tgatcgtcgc
      661 agtgatcgcg agctgtagcg gcgaaccgcc ggagcgcttg cacgtgcctg cgaagctgat
      721 cgagctcgcg ccgcttccac ctccgccccc accaccgccg cctcctccgc cactcgacac
      781 gaccgtcgag atgcccgacg agctgtcgga tgtcatctcg ccatacggca ctccaccggc
      841 cgtgacgatc gtcgagctca ccgagggaaa cgcgcacggg gtttttctcg gctacggcat
      901 cctgcgtacg aagccgctca ccgacatgca cgcgaaacag ctggtcgaca agctcgccgc
      961 tgacaacgcg ttcatcgatg gcggcgacta cggctgcgtc ggcgatccga tcggcctgcg
     1021 gttcgcgcgc ggctcgattc tgcgcgacgt ggtcgtcgac tgcggccaca tgtacctgac
     1081 gccgagccgc cacgacggcc gattcgcact gctcgctccc gacgtgaatc agttcatcta
     1141 cgcactgcgc tgagcgcatc actcggccaa cctagcgtgt gcggcgttgg tcgatcgttc
     1201 tggtggtcgt tggttgcgcg agcgcacggc ccgaaccgct cgacacgccg gcgcagcgcg
     1261 tgcgcgagcc gggaccggtc gcaccggttc gcaagccacg cttgctgatg ccgatcctga
     1321 ccgtggtcga tgcgacgacg cccgtcgagc cgcctgcgat ccggatcgac aagcgcgcgg
     1381 gcatcatggg cagaggcggc ggcggattct cggtctggta cgacggcacc gtgcagtttc
     1441 gcggtccgta ctgcgccgaa ccgcggcgcg gcaccgtcga ggcggcgcgg gtcggcgagc
     1501 tcatggcgac gctcggtcgc agcggcttgt tcgaacgcaa gcagcggccg atcgatcgcg
     1561 gttgcggcta cgcgctcgac ggttcctact gggacatcgc actgcgcgca gccgagcgca
     1621 gcaacacgtt caccggcccc gacacggcac cgcagcgcac gtgcgcgtgg gtcgaggaca
     1681 tgctcgacgc gatcggcacg gtgatcggcg cgaatccgtg cggcccatga gcgacgatta
     1741 ctcggtgcag gcggtcttgc ggccgggaat cactttgccc ggattcagga gaccggtcgg
     1801 atcccacagc gacttccact tgcgctgcca cgcgagcact tgctcgctct gctccatgtg
     1861 gaggtagtcg cgcttcgtga ggccaatgcc gtgctcgccc gacagcgtgc cgccgagctc
     1921 gaccgtgtgt gcgaacacgc gttcggccgc ggcccacatc cgcttgcgca ccgccggatc
     1981 ttgcgggtcg tcgttcgaca atagattcac gtgcaagttg ccgtcgccag cgtgaccgaa
     2041 caccgcgatc ggaatgccgg tctcctgcga tacgcgatcg acgcgcgcca gcatctccac
     2101 gatcttgccg cgcgggacgc agacgtcctc gttgaccttg atgcgccacg cttccttgag
     2161 cgcgggcgag atcatgcggc gcgcttccca caccgcgcgg cgctcggccg gttcggtcgc
     2221 accgagcaca tcgagcgcgc cgagctcgtc gcacgcgaga ccgattgctt ccatctgcgc
     2281 ggccatcgaa tcgggcgtgc catcgacctc gatgagcacg atcgcgcccg cattgtcggg
     2341 gaaccgatag cgcgtcttcg cgcgcacgca gtcgatcgag aacttgtcgg caagctcgag
     2401 cacgcgcggg cggaggccac cgcgcaacag cgacgagatt gcagcgccgg ctttcggaac
     2461 gtccgcgaac acgccgagca cggtggcggc ggcgggcggg cgcggcaaca gcttcagcgt
     2521 cagctccgtg atcacgccga acgtgccctc ggtgccgacg aacccagcga cgaggtcgta
     2581 gccggtgacg cccttcgccg tgcgccggcc gcagcgcatc acttcgccgc cgatcagccc
     2641 gacttcgagg ccgagcacat attcgcgcgt gacgccgtac ttgaacgcgc gcggacctcc
     2701 ggcattcgtc gccgcgttgc cgccgatcga gcagaactcg agcgacgcgg ggtcgggcgg
     2761 atagaacagc tcctgcgctt cgacctgctc ctgcaggcgg ccggtgacga caccgggttc
     2821 gacgacgcag acgagatcgt ccgggtcgat ctccttgatc gcttgcatgc gctcggtcga
     2881 cagcacgatg ccgccttgca gcggcaatgc accaccgacc atgccgctgc ccgcgccgcg
     2941 cggcgtgacc ggcactttgc gctcggcgca gatgcggagc acggcggcga cttgctcggt
     3001 cgagctgcac agcaccgcgc actcgggcgg aaagaattcc ggcagcggac tttcgtcgcg
     3061 gccgtagtca tcgagcttct cgtggccggg cccgatgatc gcgtcggacc cgagctcgcg
     3121 ctcgaggacg ctaacgatcg tcatgttaga cgctgaacga cgagccgcat ccacacgtgg
     3181 actttacatt cgggttctgg aacttgaagc ccgcgccctg cagtccttcg acataatcga
     3241 tctcggtgcc gacgaggtac atgaggctca tctcgtcgac aatgcacttc acgccttcga
     3301 cttcgaactg gcggtcgact tcggcgggct cgtcgaaata gaggtcgtac gagaaaccag
     3361 cgcagccgcc accgacgacg cggaggcgca gcgccatgtt gccttcgata ccctcggcct
     3421 gacggatctc gttgaccttc gaagcagcgg tcggcgtgat gcggagcatc gactccggac
     3481 cctggggatc gagcgaatcg gacatggaag cgacagcggc gtcagacatc ggggcgacct
     3541 tacctttacg tgagggttac ggcaagggct gtgctaagac tgcgacgtgc cgagctacga
     3601 cgcgctgctc cgcgagcttc gcagccaaac tcccgagatc gatgtccaag ggctgtctga
     3661 ccagctgggc aacgtcgctc tgatcgactg ccgcgaatcg gacgagtact cgcaggggat
     3721 gatcccgggc accgtgtgga tcccgcgcgg caagctagag cagaagatcg agaaagcggt
     3781 caccgatcgc agcgcgccga tcgtcatcta ttgcgcgagc ggcaaccgat cgctgttcgc
     3841 gcagcgcacg ctcgtcgagc tcggctacac gaacgtcaaa tcgctcgccg gtggattcac
     3901 ggcgtggaag cgtgccggac tgccgtggga catgccggcg acgctgcgtc ccgatcagga
     3961 aacgcgttac tcgcgccaca cgctgcttcc cgaggtcggc gtcgccggcc aacagaagct
     4021 gctcgcgggc aaggtcgtct gcatcggcgc cggtggcctc ggctcgccgt cgagcatgta
     4081 tctggccgct gcgggcgtcg gcacgctcgg catgatcgac gacgacatcg tcgacgcgtc
     4141 gaacttgcag cgtcagatcc tgcacggcac cgaccgtctc gcgacgccga aggtcgaatc
     4201 cgccgagcgt acgctgaaga acctgaaccc cgacgtgcgc gtcgagaagt atcgcgcgcg
     4261 gatcacgagc gcgaacgcga tcgatctgct cggccgctac gacgtgatca tcgacggcgc
     4321 cgacaatttc gcgacccgct acctcgtcaa cgacgtcgcg ctcaagctcg gcaagccggt
     4381 catccacgcg tcgatcttcc gcttcgaggg ccagatcacc gtgtttcccg cgaacgggtc
     4441 gccctgctac cgctgcctgt atccccagcc accgcctccc gaggacgcgc cgtcgtgcgc
     4501 ggagggtggc gtgctcggcg tcctgccggg gatcatgggc gtcctccagg ccaccgaggc
     4561 catcaagctc ctgcttggcc tcggcgattc actcgcgggg cgattgctcg tgtacgacgc
     4621 gctcagaacc cggtttcgcg agctgaagct gcgccgcgac ccgaagtgcc cgacctgcgg
     4681 cgatggcgta gatcgcaccg cgatagagct ggttgattac gagcagttct gcgcgggccc
     4741 caagcactga gctacactat caggtgcgtt gatcgccgcc gggcaatcga tcggcagcta
     4801 ccgcatcctg cagaaaatcg ggactggcgg catgggagcc gtgtacctcg ccgagcaccc
     4861 gctgatcggc aagaaggtcg cgctcaaagt cattcaccgc gagctcgcga gcaacaaaga
     4921 ggtcgtgcag cgcttcttcc aggaagcgaa ggccgtcacc aagatcggca acgagcacat
     4981 cgtcgagatc cacgacttcg gccagacccc cgagggcgac cacttctaca tcatggagta
     5041 cctcgagggg cagaccctcg cgacggtgct cgcgacggac cgcgtgctcg acgtcatgcg
     5101 tgcgctgcac atcggtgcac aggtcgctgc cgcactcgca gcggcgcacg cggcgggcgt
     5161 gatccatcgc gacctcaagc ccgacaacgt catgctgacg acgcggctcg gcgacaacaa
     5221 cttcgtcgtg ctgctcgact tcgggctcgc gaagatgttc gtcggcggcg gtcagtccgc
     5281 ggtgaagaca gcggcgggcg tgttgctcgg cacgccgcag tacatgtcgc ccgaggcgtg
     5341 cgagagcaaa ggcaacctcg atcaccgcac cgatatctac gcgctcggga ttttgttgtt
     5401 ccagatgctg accggcatgc tgccgttcga cggcgagacg atgggcgagg tcctcgtcaa
     5461 gcaagtcacg cagctgccgc caccgccgcg cggctacaac cagaacattc cacccgcggt
     5521 cgagcagatc gtgctgcgct gcctcgcgaa gagtgtcgac gcgcggttcc cgacgatgct
     5581 cgcgctgcgc gaagcgctgc tcgatcccga ggcgtatctg cgcaagtcgc cgccgatgtc
     5641 acctgcgcgc tcgctcgcgc cgggcgaggc gaaggtcgat gcgaagaccg tgatggcgta
     5701 cgccgcgcag gagcagcgca cgaagatcga catgggtggc gtcgcgccat cgcgcggaac
     5761 tgggccgcag aacgcgatgc cgctgccggc acccgcaccg tcgctgccgg gcaatgcagg
     5821 cgcgaagacg atgatcgcga gcccgggaat gccgctgcca caggctcctg caccgccacc
     5881 gcaagcgccg gagatggtcg cgccggcgca gccgaagatg aacacgatgc ggatcgcgac
     5941 gccgctcggc tactcgtcgc ggccgccgcg caagatgtgg ccgatcgtcc tcgtgttcgc
     6001 gctgctgctc gggctcggtg gcggcgcgtt tgcggtcgcg tggttcggca agaccgatgg
     6061 tgacggcggc ggtggtagcg cgtcgggtgg cggtagtgcg tccggcggca gcgcgaccgc
     6121 gagcaatcct ttgccgcccg cgccgaagcc agatgcgcag gtcgtcgtcg agaacaaagg
     6181 ctccgccgcg ccgcagaacg cgggctctgc gacgccggtg aaggtcgaca aagtcccggc
     6241 aggttccggc gccggcgcgg ccgcgggttc ggcggcgccg ggcacgccca acaatgcgca
     6301 gaagccaggc atggcggtga tcgtcatcga cagcacgcca cagggtgccg aggtcctcgg
     6361 ccccgacaag aagtcgctcg gaaaaacgcc ggtgacggtc tcgctgccga tctccgacat
     6421 gccgcaagac ttcgagctga agctcgccgg ctacaagaag aagaccaagc agatcatgat
     6481 cacgggcaac agcgtgatta acgtgatgct cgacagggcg ccgtccagcg gccgcagcgg
     6541 caagggcagc ggtaagaaga agggctccgg cgactcggaa ctgatgcggc cggacgacct
     6601 atagggcaga ctgggcctat ccctcatggt gttcgctgtc gtcgccggtc tcgtcgtgct
     6661 cgcagtcgcc gcgttcttcg cggcgcgcgt gtcagcggcc gagcagctgc ggcgcgtgag
     6721 ccgcgccatc gacgaagcgc gcatgcaggc ggccgccgaa cgtaaagcgg ccgagctggc
     6781 cgcggccgcg cagcgcacga aggccgaagc tgcagcacgc aacgaagcgc tcgaagcgag
     6841 cgcgaaggtc gacgaggagc tcgccgagcg cgaagcgaag ctcgccgagc gcgaggatgc
     6901 gctgcacgtg cggcggagcg agctcgacca tttcgaagac agcgtcgagg cgcgtgaggg
     6961 gcagctggga cagatccagc gcgacgtgca gtcgcgtcac gaccgcgcgc gcggtctcga
     7021 tcaggatgtg cagcgcaagc gcgagacgat gaagggcgag ctcgagaatc gcgccggcat
     7081 caaggcgcaa gagctcatcc gccagatgtc cgcggtgtgg atcgaagagg cgagggcacg
     7141 tgccgctgcg ctcgtacgca acgccgagca atcgaccgcg gatccgcagc acgatcgcga
     7201 cgctcgccgc gtgatggaaa tcgcggccgc tcgctgcagc atgcacttcc tcaccgagcg
     7261 caacctgtcg acgttgcgcg tcgggcccga catcgtgtcg atcctgctcg acaacaatgc
     7321 tgcgattcat ggcgcgctcg agcgcgtcgc cggcgtgcag ctgcagatca atgacgagaa
     7381 ggacgtcgtc cgactcgaag gtctcgacgg cgtcggccgc gaagtcgtgc gtcgcgcact
     7441 gtcgcgcatg atcaagaagc cggagaccat cgacgccgcg cgcaaggcgc ctgacgaatg
     7501 ggctgcgaag gcgcgcgagc atctcgagca agagatccgc gcgctcggca agaaggcgtt
     7561 ctcgacgctc ggcatccaga aggcgcatcc cgagattgtc gatctcgtcg gcgcgctcaa
     7621 cttccgcacc agctacaccc agaaccagtg gctgcacgcg gtcgaagcga gcttcctcgc
     7681 cgggatgatg gcggccgagc tcggtctcga cgagaagctc gcgcgccgcg cgacgctgat
     7741 gcacgacatc ggcaaagcgc tcacgcacaa gatcgaaggt agccacgccg tgatcggtgc
     7801 cgacatcgcg cgccggctcg gcgagtccga gctggttgcg aacgcgatcg gctcgcatca
     7861 cgctgacgaa ccgccaaact cggtctacgc gtatctcgtc gccgcctccg acgcgatgag
     7921 cggtgcgcgt cccggcgcgc gacgcgagca ggccgaaggg ttctcgacga agctcgaaga
     7981 tctcgagcgc atcggtctgc agcggcgcgg cgtcagccac gcgcatgcgg tccatggtgg
     8041 tcgcgagctg cgcgtttacg tgcgcgaaaa ggaagtcgac gatctcacag ttgtcgagat
     8101 gtcgacggag atcgcgaacc agatcgccga agagatgacg ttccccggcc agatcaaggt
     8161 caccgtcatc cgcgcgttcg aggcgacctc gacggcgaac tgattgcgcc gtagcgccac
     8221 accgcacgtt ctatcgagcg ggcacacgtc gcatcgcgcg cggacgtgca tgcgcgggag
     8281 ttgctcgtcg cgacggggca catgggatgc acgcgtcggt gcagcaacca cgagggaacc
     8341 atggcgaacc acggcaacgg acgacacgtg atcgacgacg acgatgacga ccgccacacg
     8401 tcacgtgatg accggtatgc gcgcggccgc gaggagggtc gctacgtcac gcgtgacgag
     8461 ccgcgcaacg tgccaccgag tcaacgctat gggcactggg aggatcgcac cacgcgtacc
     8521 gagcgcgacg agggctaccg caagctgcag agtcgcgagc tcgcgtatcc cggtggcttc
     8581 gagcaacgcg cgcgcgagcg tgacgacgac gacgagccgg acgacacgcg cggcggctat
     8641 ctcggccaag gtggccagca gctgggctac gtggaatcac gtcacggcca ccatcaagag
     8701 ggcgggatgt acggtcatgg cccggggacg tggggcgagc gtcgcacctc ccatcgtggc
     8761 aaaggtcctg cgaactacac gcgatctgac gagcgtatcc gcgagctggt gtgcgaagcg
     8821 ctcaccgacg atcacgatgt cgacgccacg cgcatcgagg tcaccgtgac ggacggcgag
     8881 gtcagcctcg ctgggaccgt cgaggaccgc taccaaaaac ggatggcgga gaactgcgca
     8941 gaatgcgttt ccggcgtcaa agacgttcac aaccagctcc gcatcgccgt cgacaagcag
     9001 cgccccgacc gggcgtagcg tgagagcacg ctgtagacgc tttgctcgat cttcgtcgga
     9061 gaatcgtcgg tggggcaggg gcgaatcagc tggtcttctt tatgcaccgt cgactctcgc
     9121 catggtgcct ttcttcgaag acgacgacga tccgaaaaag ccgcgcccgg tgcacgtgac
     9181 caagaagcgt gacggttggg ccgaaatgga cgaagcgcgg aaggatgacg accgcgacca
     9241 ccaccgccac gtgcacgtca cacggtgctg atgctggatc gcatcgccat atccgctccg
     9301 actctgcgca tcgtctgcgc agcgttgctg atgttgttga tcgcgcgtgt cgactttttc
     9361 gcgtccgcgc tcgcatggca ggtacgcggc gaagtcgccg gctcggcact tcactgtccg
     9421 catcgagtcg tgcatccggt ggtgcgcgcg cgcccgcaaa tgcggacgct cggcatgcat
     9481 caccgctaca tgatgcgcga tgacgtcggc cgccgtcggc tgttacagct cgatcagtga
     9541 tcagcgcgcg agcagctcgc cgagcttgtc gagtgcttgg ttccagccgg cgccggcgcc
     9601 gccgatgtag cgcttgggat ccatgcccga caccagcacg gtcatcgtca gcgtcagtac
     9661 cgtcttgccg tcgcgctcgc cgaacgtgac cgtattgagg tgctcggaca tgagcttgcc
     9721 gtcatgctcg ctgcgttcgg tgtacgcgag ccgttcgccg tcgatgacct cggtaaacgc
     9781 gccgacaatc gtcatcgcca tgccttcgcg gcgcatgtcg tagcgtagag cgccgcctgg
     9841 ccggaggtcg aactcgacgc gcggcacgac catgccgtgt gggccgaacc acttcgcgaa
     9901 ctgcgtcgtc tccgtccacg ctttccagac gagcgcgcgc ggtgcagtga attcgcgctc
     9961 gatcgtgagc tgcgcgagcc cgccgacgat ctcctcgatc ttgtcgtagc ccttcgccgc
    10021 gcccttctcc atgcccgact ggagcacgat gtcgcgcgtt tgtttgctgt cgtacttcgc
    10081 gacgagccgc atcgtcgtct tgccgttgtt ctcggtgaac gtcgtcgtga tcaggctcgg
    10141 atgctcgcgt gccatgtcga cgtcgaagat ctcggtgtac acgaccttct cgggatagac
    10201 gatttcgtgg tgctcgccgc agaacggcac ctcgcggccg tcaggcgtct tgccgacgta
    10261 gcggtacttg ccgccgacgc gaaagtcgat ctcgcacacg gtcatctcga cgtggccggt
    10321 gccccaccac tgccgcacgt gctcgggttt ggtgaacatc tcccagacga gcgcgcgcgg
    10381 cgcgtcgaag tctcgcgtca tctcgatctc gagatccgac ggcgtcgtga caaccagttt
    10441 atttcttgcg ctcatctttc ttgtccttct gctggatggt tttcaggtag tcgtcgaggc
    10501 ggtcgaagct cgcttcccag aactgccggt atttctcgag atacacagac acctcctgca
    10561 ggggccgtgc atcgagtcga cacggtcgcc actgcgcatc gcgcccgcgg atgatcagcc
    10621 ccgcacgctc gagcaccttc aggtgcttcg agatcgcagg caggctcatc tcgaagggag
    10681 cggcgagctc ggtgaccgac acgccgtctt gcccctccgc taggcgggca aggatcgcgc
    10741 gcctcgtcgg atcggcgagg gcagcgaatg tcgtatcgag attcttcgac atcttcgatt
    10801 taacctcgta gttaattaac ccataggttc aatagtcgca gaagtcaatc gcacgttaag
    10861 gtgaggtttc ggattgacac tgaagcatca cccgaccata gtcccttcag ctagatagaa
    10921 ccttacgtat acgtgagggt taagcatgga cgagagcaat ccacgacaca ggtctgcggc
    10981 cgacaagctg atgcgcgtcg gcgagctcgc agccgcggtc aacaagaccg tccgcgcgat
    11041 gcacctctac gaggagctgg gattgctcga gccgcgtgcg cgctccgaag gtggcttccg
    11101 actctatggt cccgaagcga tcgatcgcat ccactggatc gtgaagctgc aggcgatcgg
    11161 attcacgctc gccgagatcc agggcttcgt gaaggatttc cagagcgcgg gttctgcgcc
    11221 cgaagcgacg gcgcgcgtac gtgcgctgtt ccaggagaag cagcagaaca ttcgcgagca
    11281 gatcacgcag ctttcggtga tcgagaacga catcaacgaa gcgctcgcgt atctcgatag
    11341 ctgccagacc tgttcgactg actacgcgcc gaccgagtgc ggcgcctgcg atcaccaggg
    11401 gcaccagaag ggcgaagcgc cgccactgtt cgcaggaatc tccaagcgtc acgaggaaaa
    11461 ggaattcgat gttcccgtga ccgtgctcta tcgcgaagga aacaactgat gtctgaggtc
    11521 aagacgccca tcttcatgga caaccactcg acgacgcgcg tcgatccgcg cgtgctcgag
    11581 accatgctgc cgtacttcga cgtgcactac ggcaacgcgg cgagccgcaa ccacgtgttt
    11641 ggttggacgg cggaagccgc ggtcgagaac gcgcgtaacc aggtcgcgtc gctgatcggc
    11701 ggcgacggca aagagatcgt gtggacgagc ggcgcgaccg agtcgaacaa cctcgcgctg
    11761 aagggtgccg cgagcttcta caagaagcag ggcaaccaca tcatcaccgt cgagaccgag
    11821 cacaagagcg tcatcgatac ctgcaagcgt ctgcagcgcg agggctacga ggtcacgtac
    11881 ctcacgccgc agaaagacgg ccttgtcacg cccgagatgg tgaccgcggc gatgaccgac
    11941 aagacgctgg tcgtcagcat catgctggcg aacaacgaga ttggcgtcgt ccaggacgtc
    12001 aacgcgctcg gcgcggcgat caaggcgaag aaccccaaga cgctgttcca catcgacggc
    12061 gtccagggcg ccggcaagat tccgttcgac gtcaatgcgg cgaaggctga cctcgtcgcg
    12121 ttgtcggcgc acaagatgta cggaccgaag ggcgtcggcg cgctgtgggt gcgccgcaag
    12181 ccgcgcgtcc gcatcgaccc gatcatcgac ggtggtgggc acgagcgcgg tatgcgctcg
    12241 ggcacgctgg cggtgccgct gatcgtcggg ttcggtaagg ccgctgcgct tgcgaaagaa
    12301 gagatgcaag ctgaggcgat tcggctcaag gcgctccgcg agcggctcta ccgcggcatc
    12361 accgcgaaat tgccagagac gtacgtcaac ggttctctgg aagatggccg caggctgccc
    12421 ggcaacctga acatctcgtt cgcgttcgtc gagggcgaga gcctgctcat ggcgctcaag
    12481 gatgtcgcgg tgtcgagtgg ctcggcgtgc acgtcggcat cgctcgagcc gagctacgta
    12541 ctgcgcgcgc tgggcgtcgg tgacgagctg gcccatactt cgattcgctt cggccttggt
    12601 cggttcaaca ccgaggcgga agtcgactac gtgatcgacc tcgtcgtgaa gtcggtcgat
    12661 cggctacgtc agctcagccc gctctgggaa atgcatcaag aagggatcga catcaactcg
    12721 atccagtgga ctccgcactg aggaaacgac catggcgtat tcagacaaag tactcgacca
    12781 ctacgagaac ccccgcaacg tcggcacgtt cgccaaggac gaggccgaag atgtcggtac
    12841 ggggctcgtc ggcgcgccgg cctgcggcga cgtcatgcgt cttcagatca aggtcaagga
    12901 cggtcgcatc gaggatgcga agttcaagac gttcggttgc ggctcggcca tcgcgtcgag
    12961 ctcgctcgcg accgagatga tcaagggcat gacgctcgac caggcggcgt cgatcacgaa
    13021 cacccagatc gtcgaagagc tcaacctccc gccggtcaag atccactgct cggtgctcgc
    13081 cgaggacgcg atcaaggcgg cgatcgccga cttcaagaag aagaaggcgt cacgcgaggt
    13141 gaaggcggag acgccggcgg ccgaggcgcg ccgccaggag ggctgagcca tggccatctc
    13201 gatcaccgac gcggcggtga aggagatcaa gaagcagcag gcgaagcgcc agacgccgga
    13261 ctcggcactg cgcgtcggga tccgcggcgg cggctgtacc gggttctcgt acctcttcga
    13321 gtgggccgac ggcccgccca aggag
//
LOCUS       DASUFX010000491         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1607338, whole genome shotgun sequence.
ACCESSION   DASUFX010000491 DASUFX010000000
VERSION     DASUFX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607338"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFV99_19750"
     CDS             complement(<1..329)
                     /locus_tag="VFV99_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061617.1"
                     /translation="MNPSAALQHDRVGPYQLIKAIGAGGSARVDLARIERAYGFQRHV
                     VIKRPLEHLRDDPRAAASLHREARIGGLLRHPNLVTVLDAGRHDGYDYLALEYVHGAS
                     LRALMQT"
     gene            complement(326..1246)
                     /locus_tag="VFV99_19755"
     CDS             complement(326..1246)
                     /locus_tag="VFV99_19755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061618.1"
                     /translation="MRLGRYEVDELLAKGGMGEVYRGRLVAAHGFARPLVIKMIRTEL
                     LANERAALMFIDEARMAAGLHHRNILQIVDFDRFDDGAFIVTELIEGCDLRVMLTHLR
                     APPRYEIALTIISELAAGLHAAHEATGNDGSPLNLVHRDVSPSNVLLGRQGEIKLSDF
                     GVAKARLRSYHTVSGSIKGKAPYMAPEQILGEQVDRRADVFSLGVLLFEVTTRSRLYS
                     GKGDATAMKLIIDGYVPEPAELRPGYPAELAAIIRRALARDPDARYPTALAMIEEIEA
                     VARQRRWNLSCAAVGEIVRVIEQRAKETAS"
     gene            complement(1265..2140)
                     /locus_tag="VFV99_19760"
     CDS             complement(1265..2140)
                     /locus_tag="VFV99_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061619.1"
                     /translation="MRRLLLIVCMLCACDDARPRTPRLGRIKAGAEALPTSFDSTAKE
                     KAPPPTLTELVEGGTHWRFTTKHGPVHVWIPKGYNAKRAETIVYVHGFYVHVDDAWKT
                     YKLETQFAGSAINAMFIACEAPASGAEPVSWPVLADLLAEVERGIGQPWPKRRIVTIG
                     HSGAWRTLVGWLDEPQIDTVVLVDAAYGEIDEYKKWVLASDAHRLIDIGDDTRAWTDK
                     LHAELPETYVLDDFPSLEDGIPREAQRARIVYIKSHMGHFPLVTSGTALPMILRTLRA
                     RRLVREPLAEILQSE"
     gene            complement(2144..2410)
                     /locus_tag="VFV99_19765"
     CDS             complement(2144..2410)
                     /locus_tag="VFV99_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061620.1"
                     /translation="MKKYAFVIALALAACSKKKEEPPPAPPPTPVATADAAAAEGSGS
                     AGSAAPVADIEVPTEVDFEDQASTDITDKNVEAHVKAIEQELSQ"
     gene            complement(2407..>3564)
                     /locus_tag="VFV99_19770"
     CDS             complement(2407..>3564)
                     /locus_tag="VFV99_19770"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5061621.1"
                     /translation="VWVAGRWDWRGKWVWVAGHWERERAGKRWRDGRWDHKDNKWVFV
                     AGDWVDAAAQPPPVATPPVPPPVVAGPTEAPPPPREEKWTPRAGFVWVAGQWDWKAGK
                     WEWVAGHWERERAGKKWRPMRWEQKDGKWARLDGDWIDANAPDPGPPPGPPGPPQRDH
                     RQWKLERPVVSSYWPTKGKAGSRIVIRGKNFPTSAEVVFGGQAVRGAKVTAEQVVFVV
                     PPGAASGAIDLRVGGRRALPVGQFEVAAAFDPVAEQKRIEEEQRKAAEAAWATRQQQL
                     AKDRAAREAAMKQAMAERAATRERRRAERIAALQAKFKREFLADPETQDELTLHAQRI
                     AELARSRDIAEVSANGKLVVRIDVAQSREEQRHDQRMAALEASFKARGGQP"
BASE COUNT          522 a         1260 c         1147 g          635 t
ORIGIN      
        1 tcggtctgca tcagcgcgcg cagcgacgcg ccgtgcacgt attcgagggc gaggtagtcg
       61 tagccgtcgt ggcgcccggc gtcgagcacg gtcaccaggt tcgggtgccg cagcaggccg
      121 ccgatgcgcg cctcgcggtg cagcgacgcg gccgcgcgcg gatcgtcgcg caggtgctcg
      181 agcggccgct tgatcacgac gtggcgctga aagccgtagg cgcgctcgat gcgcgcgagg
      241 tcgacgcgag ccgaaccacc ggcgccgatc gccttgatca gctgatacgg accgacgcga
      301 tcgtgctgga gtgcagcgct cggattcacg acgccgtttc cttcgcgcgc tgctcgatta
      361 cgcgcacgat ttcgccgaca gctgcacacg acagattcca ccgccgctgc cgcgcgaccg
      421 cctcgatctc ttcgatcatc gcgagcgcag tcggataccg cgcgtccgga tcgcgtgcga
      481 gcgcgcgccg gatgatcgca gcgagctccg ccggataccc cggacgcagc tccgccggct
      541 cgggcacgta gccgtcgatg atcagcttca tcgcggtcgc gtcgcccttg cccgagtaga
      601 gccgcgaccg cgtcgtcact tcgaacagca gtacgccgag actgaacacg tcggcgcgcc
      661 gatcgacttg ctcgccgagg atttgctcgg gagccatgta cggtgccttg cctttgatcg
      721 aaccgctgac ggtgtgatag ctgcgcagcc tcgccttcgc gacgccgaaa tccgacaact
      781 tgatctcgcc ctgacggccg agcagcacgt tgcttggact cacgtcccgg tgcacgaggt
      841 taagtggtga accatcatta ccggtcgctt cgtgcgcggc gtggagcccg gcggccagct
      901 cgctgatgat cgtgagggca atctcgtagc gcggcggcgc ccgcaagtgc gtgagcatga
      961 cgcgcaggtc gcagccttcg atcaactcgg tcacgatgaa cgcgccgtcg tcgaagcggt
     1021 cgaaatcgac gatctggagg atattacggt ggtgcaggcc cgcggccatg cgtgcttcgt
     1081 cgatgaacat cagcgccgcg cgctcgttcg cgagcagctc ggtccggatc atcttgatca
     1141 cgagcgggcg cgcgaagccg tgcgctgcga cgagccgacc gcgatagacc tcgcccatac
     1201 cgcccttcgc gagcagctcg tcgacctcgt atctgcccag gcgcatgagc acgcccagtg
     1261 gatgctactc ggattgcagg atctcggcga gcggctcgcg aacgaggcgc cgcgcgcgca
     1321 acgtgcgcag gatcatcggc aatgcagttc cgctcgttac gagtgggaaa tggcccatgt
     1381 gcgacttgat gtagacgatg cgcgcgcgtt gagcctcgcg cggaataccg tcctcgagtg
     1441 acggaaaatc gtcgagcacg tacgtctccg gcagctcggc gtgcaacttg tccgtccacg
     1501 cgcgggtgtc atcgccgatg tcgatcaagc gatgcgcgtc ggaggcaagc acccacttct
     1561 tgtactcgtc gatctcgccg tacgcggcgt cgacgaggac cacggtgtcg atctgcggct
     1621 cgtcgagcca gccgaccaac gtgcgccacg cgcccgagtg gccgatcgtc acgatgcggc
     1681 gcttcggcca cggctgaccg atgccgcgct cgacctcggc gagcaagtca gcgagcactg
     1741 gccacgagac tggctccgct cccgacgccg gcgcttcgca cgcgatgaac atcgcgttga
     1801 tcgcggaccc ggcgaactgc gtctcgagct tgtacgtctt ccatgcgtcg tcgacgtgca
     1861 cgtagaaccc atgcacgtag acgatcgtct cggcgcgctt cgcgttgtat cccttcggga
     1921 tccagacgtg cacgggcccg tgcttcgtcg tgaaccgcca gtgggtgccg ccctcgacga
     1981 gctcggtgag cgtcggcggt ggcgccttct ccttcgcggt cgagtcgaac gacgtcggta
     2041 gcgcctccgc gccggccttg atccggccca gtcgcggagt gcgcggccgt gcgtcgtcgc
     2101 acgcacacag catgcaaacg atgagcagca ggcgtcgcac gtgctactgc gagagctcct
     2161 gttcgatcgc cttcacgtgc gcctcgacgt ttttgtcggt gatgtccgtc gaggcttggt
     2221 cctcgaagtc gacctcggtc ggcacctcga tgtctgccac cggcgccgca ctaccggcgc
     2281 tgccgctgcc ctccgcagca gctgcgtcgg ccgtcgcgac gggtgtcggc ggcggtgcgg
     2341 gtggcggctc ttccttcttc ttgctgcacg cggcaagcgc gagcgcgatg acaaatgcgt
     2401 acttcttcat ggctgacctc cacgcgcttt gaagctggcc tcgagtgcgg ccatgcgctg
     2461 atcgtgacgt tgctcttcac gcgactgcgc gacatcgatg cgaacgacga gcttgccgtt
     2521 cgcagacact tccgcgatgt cccgcgaccg cgcgagctcg gcgatgcgct gtgcgtgcag
     2581 cgtgagctcg tcttgcgtct ccggatccgc gaggaattcg cgcttgaact tcgcctggag
     2641 cgcggcgata cgctcggctc tgcggcgctc gcgcgtcgcg gcgcgttctg ccatcgcctg
     2701 tttcatcgct gcttcacgcg ctgcccggtc cttcgcgagc tgttgttgcc gcgtcgccca
     2761 cgccgcttcg gcagccttgc gttgttcttc ttcgatgcgc ttctgctcgg cgaccggatc
     2821 gaacgccgcg gcgacctcga actggccgac cggcaaagca cggcggccac cgacgcgcag
     2881 atcgatcgca ccgctcgccg cgcccggcgg cacgacaaac acgacctgct cggccgtgac
     2941 cttcgcaccg cgcacggcct gaccgccgaa cacgacctcg gcgctcgtcg ggaagttctt
     3001 gccgcggatc acgatgcggc tgcccgcctt gcccttcgtc ggccagtagc tgctaacgac
     3061 cggacgctcg agcttccact ggcggtgatc gcgctgcggc ggtcccggcg gcccaggcgg
     3121 tggtcccgga tccggcgcgt tcgcatcgat ccaatcaccg tcgaggcgcg cccatttgcc
     3181 gtctttctgc tcccagcgca tcggccgcca tttcttgccc gcgcgctcgc gttcccagtg
     3241 tccagcgacc cattcccatt tcccggcctt ccaatcccac tgtcccgcga cccacacgaa
     3301 gccggcgcgc ggcgtccact tctcttcgcg cggcggcggc ggtgcttcgg tcggacccgc
     3361 gacaacaggc ggggggaccg gtggcgtcgc gaccggcggc ggttgggcag cggcgtcgac
     3421 ccaatcgccg gcgacgaaca cccacttgtt gtccttgtga tcccagcggc cgtcgcgcca
     3481 gcgcttaccg gcgcgttcac gctcccagtg gcccgcgacc cacacccact tgcctctcca
     3541 gtcccaacgt ccggcgaccc acac
//
LOCUS       DASUFX010000492         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1607361, whole genome shotgun sequence.
ACCESSION   DASUFX010000492 DASUFX010000000
VERSION     DASUFX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607361"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1183)
                     /locus_tag="VFV99_19775"
     CDS             complement(110..1183)
                     /locus_tag="VFV99_19775"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5061622.1"
                     /translation="MEGTLSLFDWRPAPAAPQERAERPPVPAEVAAPAAPRIIVFDCE
                     TTGIDFTRDQVIELCVQHGLDDLARSQTWRIKPAVPIHPAAQAVHGITMADLAECPPF
                     AAFADEIAAVFATADVIVGYNLSFDIDMLQAELTRIGKPLLDLSGKQIVDAFRLWQQC
                     EPRSLTHAHRRFVGDEFAAAHSASADVAATGRVLVGMLRHFNLHEQDWGSIANVCDPG
                     RASWVGPSRHLRWENDVIVLGFGKHMGAAVHELAAGPDRSFLQWVLGKDFPPHVAEIC
                     RAALEQRGEDFIAWARKQFGQAAPTALPPFAQLPVSASLPAAVVAPAPVLAAGSGPVE
                     GEASITPITNGKKRRPRNQPTSP"
     gene            1236..1856
                     /locus_tag="VFV99_19780"
     CDS             1236..1856
                     /locus_tag="VFV99_19780"
                     /inference="COORDINATES: protein motif:HMM:NF023669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061623.1"
                     /translation="MRSAVILLTLAACKVKDPPPVTEEWKDSFDRDDIGGDYYKSGPG
                     YDIVSGSLSAKGAHNHPLWLRKKLPRDVRIDFDAWSNEERGDVKVEVFGDGRSYDPDG
                     GAYMATGYEVIFGGWFNSKSIIARLDEHGKDVVTNTTVKVQPTRHYHWRIERVGKKLT
                     WYVDDMQTPFLVLDDPSPLENQGHEYFGFNNWESDTWFDNLVITPL"
     gene            1878..>2999
                     /locus_tag="VFV99_19785"
     CDS             1878..>2999
                     /locus_tag="VFV99_19785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061624.1"
                     /translation="MEQFGKYTLIRKIGTGGMAEVYLARTTVAQGLNKTLVIKKIHTA
                     YARSRQFVTMFVDEAKIALGLNHPNIIQVFDFGAVGDTYFLAMEYVEGMDLLRVLQEA
                     AKARVRLPYGLSGYIVQQLAKGLDYAHRKTDEFGQPLGIVHRDISPQNILLSWDGGVK
                     IVDFGIARARDVHEEQGVIKGKFAYMSPEQARGEPVDCRSDVFAAGIVLFELVCARPL
                     FHGKGKEALEMVKSGAIPRPRDFAPELSDTLERIILRALSFHRADRFQTARDLQHELQ
                     KFQLEWGSKTGSLIDSGSLAAQLAALVPPEQRVVAPRPVVEGDRVPSAVDGDASQPNP
                     MLIEVSGPAKAPVSHQTHNTGTPVPGRTSAREGRERKYVY"
BASE COUNT          530 a         1019 c          968 g          482 t
ORIGIN      
        1 gtcgtgtact gcttcgtgtt cctgctgttc tgcatccgcg gcgccggccc gatctcgatc
       61 gatcgccagc tcggccgcgc gtaacccccg cgaagcccaa gcgccgctgt cagggcgacg
      121 tgggctggtt gcgaggccga cgcttcttgc cgttcgtgat cggcgtgatc gacgcctcac
      181 cctcgacggg ccccgagccc gctgcgagca ccggcgctgg cgcaaccacg gccgctggca
      241 atgaggcgct caccggcagc tgcgcaaacg gcggcagcgc ggtcggcgcc gcttgaccga
      301 actgcttgcg cgcccacgcg atgaaatcct cgccacgctg ctcgagtgcc gcgcgacaga
      361 tctcggcgac gtgcggcggg aagtccttgc cgagcaccca ctgcaagaag ctgcgatccg
      421 gaccggcggc gagctcgtgc accgcagcac ccatgtgctt gccgaagccg agcacgatga
      481 catcgttctc ccagcgcagg tgacgcgacg ggccgaccca gctcgcgcgc cccggatcgc
      541 acacgttcgc gatcgagccc caatcctgct cgtgcaggtt gaagtgacgc aacatgccga
      601 cgagcacgcg gccggtcgcc gcgacatctg cgctcgcgct gtgcgctgcc gcgaactcgt
      661 cgcccacgaa gcggcgatgc gcgtgcgtga ggctgcgcgg ctcgcattgc tgccacaacc
      721 ggaacgcgtc gacgatctgc ttgcccgaca gatcgagcag tggcttacca atccgcgtga
      781 gctcggcctg cagcatgtcg atgtcgaacg acaggttgta gccaacgatc acgtcggccg
      841 tcgcgaacac cgctgcgatc tcgtccgcga acgccgcgaa cggcgggcac tccgcgaggt
      901 ctgccatcgt gatgccgtgg accgcttgcg ccgcaggatg gatcggcacg gccggcttga
      961 tccgccaggt ctggctgcgc gcgagatcat cgaggccgtg ctgaacgcac agctcgatga
     1021 cctggtcgcg ggtgaagtcg ataccggtgg tctcgcaatc gaacacgatg atgcgcggcg
     1081 cggccggcgc agctacctcg gccgggacgg gaggccgctc ggcacgttcc tgtggcgccg
     1141 ctggcgcggg gcgccaatca aacagcgaca gtgtgccttc cacaccaaaa gcctacggtg
     1201 cccctatgac agcccatttt cggtagtctc cgcgcatgcg ttccgccgtc atcctcctca
     1261 cgttggctgc atgcaaggtg aaggatcctc cgccggtcac cgaggagtgg aaggacagct
     1321 tcgaccgcga cgacatcggc ggcgattact acaagtccgg gccgggttac gacatcgtga
     1381 gcggctcgct atccgccaaa ggcgcccata accacccgct gtggctgcgc aagaaactcc
     1441 cgcgcgacgt gcggatcgac ttcgatgcct ggtcgaacga ggagcgcggc gacgtcaagg
     1501 tcgaggtctt cggtgacggc cgctcgtacg atcccgatgg cggcgcgtac atggcaaccg
     1561 gttacgaggt gatcttcggc ggttggttca acagcaagtc gatcatcgcg cggctcgacg
     1621 agcacggcaa agacgtcgtg acgaacacca cggtcaaggt tcagcccacg cgccactacc
     1681 actggcgaat cgaacgggtc ggcaagaagc tgacgtggta cgtcgacgac atgcagacgc
     1741 cgtttctcgt cctcgacgat ccgagcccgc tcgaaaatca aggccacgag tatttcggct
     1801 tcaacaactg ggagtcggat acctggttcg acaacctcgt gatcacgccg ctctaggcta
     1861 aaatcagctc ttcgagggtg gagcagttcg gcaaatacac gctgatccga aagatcggca
     1921 ccggcggtat ggccgaggtc tatctcgcgc gtaccaccgt ggcccagggt ctgaacaaga
     1981 ctctggtcat caagaagatc cacaccgcgt acgcgcggag ccgccagttc gtcacgatgt
     2041 tcgtggacga ggcgaagatc gcgctcggtc tcaaccaccc gaacatcatc caggtcttcg
     2101 acttcggtgc cgttggcgac acgtacttcc tcgcgatgga gtacgtcgaa ggcatggacc
     2161 tcctgcgcgt gttgcaggag gccgcaaagg cgcgcgtccg cctgccctac ggcctgtccg
     2221 gctacatcgt ccagcagctc gcgaagggcc tggactacgc ccaccgcaag accgacgagt
     2281 tcggccaacc gctcggcatc gtccaccgcg atatctcgcc gcagaacatc ctcttgtcgt
     2341 gggacggcgg cgtgaagatc gtcgacttcg gcatcgcccg cgcgcgcgac gtccacgaag
     2401 agcaaggcgt catcaagggc aagttcgcgt acatgtcgcc cgagcaggcg cgcggcgagc
     2461 ccgtcgattg ccgcagcgac gtgttcgccg ccggcatcgt gctgttcgag ctcgtgtgcg
     2521 cgcgcccgct gttccacggc aagggcaaag aggcgctcga gatggtcaag agcggcgcga
     2581 ttcccaggcc gcgcgacttc gcgcccgagc tgtccgatac gctcgagcga atcattctgc
     2641 gcgcgctgtc gttccaccgc gccgaccgct tccagaccgc gcgcgacttg cagcacgagc
     2701 tgcagaagtt ccagctcgag tggggcagca agaccggctc gctgatcgat tccggatcgc
     2761 tcgcggccca gctcgctgca ctcgtgccgc cggagcagcg tgtcgtcgcg ccgcggccgg
     2821 tcgtcgaggg tgatcgggtg ccgtcggccg tcgatggcga cgcgtcgcag ccgaacccga
     2881 tgctcatcga ggtgagcggg ccggcgaagg cgccggtctc gcatcagacg cacaacaccg
     2941 gcacgccggt gcccggccgc acatcggcgc gcgagggccg cgagcgcaag tacgtctac
//
LOCUS       DASUFX010000493         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1189620, whole genome shotgun sequence.
ACCESSION   DASUFX010000493 DASUFX010000000
VERSION     DASUFX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189620"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..990)
                     /locus_tag="VFV99_19790"
     CDS             complement(34..990)
                     /locus_tag="VFV99_19790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5061625.1"
                     /translation="MAAEPGVDSRRRARRALHDDHERQRLPDRGGSLKSRKLALSTGL
                     TYHVLEWGADTSPADTTFVLVHGFLDLGFGWHEVAERLATRVHVVAPDLRGHGDTDWI
                     GAGGYYHFLDYVADLDELIARLARKRLVVVGHSMGGGVSSYWAGTRPTRLSALALLEG
                     IGPPDQSEVELTGRTAQWIDAWRTARSKQKPMASLDEAAARLRKHDKLLGEALARKLA
                     AAGTREVESGLVWKHDPLHLTMGPYPFRRDAAAQYWKRITCPVLVVDGAETMMNLPEP
                     ERATRRAYFANHRHVVLPGAGHMMQRHQPDALAQLLLELELA"
     gene            complement(891..2117)
                     /locus_tag="VFV99_19795"
     CDS             complement(891..2117)
                     /locus_tag="VFV99_19795"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5061626.1"
                     /translation="MRNTALVLVLAAACGGGGDNGKKDAGNPPADSPPAIDAPPLAGC
                     TPVSGTTVKVRLIGKVSGGAMLATSPPNDGRLFVVEQQGRIRIFENEMLKTTAFLDVT
                     GATGFVAGGEQGLLGLAFHPNYANNRQFYVFYTTNNANVVAKYTQSATDPNIADATGE
                     VLLSIPDFASNHNGGMMEFGSDGYLYISTGDGGNANDPQRTAQDPNKLLGKILRIDVN
                     TTSGQKPYGIPAGNPFATSGGAPEIFMLGLRNPWRWSFDRMTGDMWIGDVGQGTDEEL
                     DVLKPSEQPGANLGWSMYEGTRCFGNYTCDPTGKFMPQATRAASTNWHAIIGGQVYRG
                     SCFPDLQGTYFFTDNSAVQLQTAKLDAGGTVTTANLPAPQGGWPPSPASIHADARGEL
                     YMTTTSGNVYQIEAGP"
     gene            complement(2156..2518)
                     /locus_tag="VFV99_19800"
     CDS             complement(2156..2518)
                     /locus_tag="VFV99_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061627.1"
                     /translation="MAELPNADDAFDVKKQDAIAEAISHLSKEEAALFLFTLEMKLRK
                     RKLQLVGYLVAMAVWLAGMLFALVIFGMASGFVGWVFLVPFALVGVTMWGFGKWAEKV
                     GKTAPPADLVEAVQRQRK"
     gene            2553..3392
                     /locus_tag="VFV99_19805"
     CDS             2553..3392
                     /locus_tag="VFV99_19805"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX5061628.1"
                     /translation="MPTLKVTDDLGRALLFAHPPQRIVSLVPSDTHSVIALGAGDRLV
                     GRTSWCEHGDGVPTVGGTKDVDVDAVMALEPHLVIANQEENPRKPLEALAERGVQVLV
                     SLPRRVDQGIAHLARLARILGVAEAPAVRELIKRGYELPAAPPTRQRAFIPIWMDPLM
                     TLNADTFGSDVLARVGVENAFGDRLRLYPLAADLGKTAPQDAGNRDVRYPRIAKEEIQ
                     ARGADLIILPDEPHAFSADDEAELHAVLPKARVVRVNGKDLFWYGAWTIEAIPRLSAQ
                     LTN"
     gene            complement(3382..>4222)
                     /locus_tag="VFV99_19810"
     CDS             complement(3382..>4222)
                     /locus_tag="VFV99_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061629.1"
                     /translation="RSGAGAVKLRVATCKTLPEEDADEAPLSAALAAAGIDAPLVAWD
                     DPSADWDAPIPTIIRSTWNYALQLDAFLAWVDRAAAAAPLLNPPDIVRANVRKRYLLE
                     MQARGVPVVPTKLVERGEICELPATRIVIKPEIGAGSLHTKSFADGRNGIDHLRFVTS
                     LGAALVQPYVASVDDYGERSLIWIDGEFTHAIRKTPRFAGDIERIEGPFPIADNERAV
                     ATAAIAPYVDRILYGRVDMVRDPDEQPMVAELELVEPSLFFPKYPPALDRFIAGLERR
                     LNW"
BASE COUNT          685 a         1366 c         1446 g          725 t
ORIGIN      
        1 gtcacggcgc ccgaagacgt catcgatccg ccgtcaggct agctcgagct cgagcagcag
       61 ctgcgcgagc gcgtcgggtt gatggcgctg catcatgtga ccagcgccgg gcagcacgac
      121 atgccggtga ttcgcgaaat acgcgcggcg cgtcgcgcgc tcgggctccg gaaggttcat
      181 catcgtctcg gcaccgtcga cgacgagcac gggacacgtg atgcgcttcc agtactgcgc
      241 ggcggcatcg cggcggaacg ggtagggacc catcgtcagg tgcagcgggt cgtgcttcca
      301 caccagaccg ctctcgacct cgcgcgtgcc ggccgcagcg agcttcctcg cgagcgcctc
      361 gccgagcagc ttgtcatgct tgcggagccg cgcggctgcc tcgtcgagcg acgccatcgg
      421 tttctgcttg ctgcgggcgg tacgccacgc gtcgatccac tgcgcggtgc ggccggtgag
      481 ctcgacttcg ctctgatcgg gcgggccgat gccttcgagc agcgcgagcg cggagagtcg
      541 cgttggccgc gtgcccgccc agtacgacga cacgccgccg cccatcgagt ggccgaccac
      601 gacgagccgc ttgcgtgcga gccgtgcgat cagctcgtcc agatcggcga cgtagtcgag
      661 gaagtggtag tagccaccgg cgccgatcca gtcggtgtcg ccgtgaccgc gcaggtcggg
      721 cgcgaccacg tgcacgcgcg tcgccagccg ctcggcgact tcatgccagc cgaagccgag
      781 atcgaggaag ccgtggacga ggacgaacgt cgtgtcggcg ggactggtat ctgcccccca
      841 ctcgaggacg tgatacgtga gccctgtcga gagcgcgagc ttgcgtgact tcagggaccc
      901 gcctcgatct ggtagacgtt gccgctcgtg gtcgtcatgt agagctcgcc gcgcgcgtct
      961 gcgtgaatcg acgccgggct cggcggccag ccgccttgtg gcgccggaag gttcgcggtg
     1021 gtgaccgtgc cgcccgcatc gagcttcgcg gtctgcagct gcaccgcgga gttgtcggtg
     1081 aagaagtacg tgccctgcag atcggggaag cagctgccgc gatagacctg gccgccgatg
     1141 atcgcgtgcc agttcgtgct cgcagcgcgc gtcgcttgcg gcatgaactt gccggtcgga
     1201 tcgcacgtgt agttgccgaa gcagcgcgtg ccttcgtaca tgctccaacc gaggttcgcg
     1261 ccaggctgct cgctcggctt caacacgtcg agctcttcat cggtgccttg gccgacatcc
     1321 ccgatccaca tgtcgccagt catcctgtcg aacgaccacc gccacgggtt gcgaaggccg
     1381 agcatgaaga tctcgggcgc gccaccgctg gtcgcaaatg ggttgccggc cggaattcca
     1441 tagggcttct ggcccgacgt cgtgttgacg tcgatgcgga ggatcttgcc gagcagcttg
     1501 ttcgggtctt gcgcggtgcg ctgaggatcg ttggcattac cgccatcgcc ggtgctgatg
     1561 tacagatagc cgtccgagcc gaactccatc atgccgccgt tatggttgct cgcgaagtcg
     1621 gggatcgaga gcagcacttc gcccgtcgca tcggcgatgt tcggatcggt cgcgctctgc
     1681 gtgtacttcg cgacgacgtt cgcgttgttc gtcgtgtaga agacgtagaa ctggcggttg
     1741 ttggcgtagt tcggatggaa cgcgaggccg agcaggcctt gctcgccacc ggcgacgaag
     1801 cccgtcgcgc cggtgacgtc gaggaacgcc gtggtcttca gcatctcgtt ctcgaagatg
     1861 cggatgcggc cttgctgctc gacgacgaac aggcggccgt cgttcggcgg cgaggtcgcg
     1921 agcatcgcgc cgccgctcac cttgccgatc aggcgaacct tgacggtcgt gccgctcacg
     1981 ggtgtgcagc cggccagcgg aggcgcatcg atcgccggtg gactgtcggc gggcggattg
     2041 ccggcatcct tcttcccgtt atcgccaccg ccgccacagg ccgccgccaa gacgagcacc
     2101 aaggccgtgt tcctcatgcg cccgatagta acgcaagccc gcgaaccggc ggctcttact
     2161 tccgctgacg ctggacggcc tcgacgaggt ctgcgggagg cgccgttttg ccaaccttct
     2221 cggcccattt gccgaatccc cacatggtca cgccgacaag cgcaaacgga acgaggaaca
     2281 cccaaccaac aaagcctgat gccatgccga agatgacgag ggcgaatagc attccggcga
     2341 gccagaccgc catcgcgacg agatagccga cgagctgcag cttgcgctta cgcagcttca
     2401 tctcgagcgt gaacaagaac agcgccgcct cttccttcga cagatgcgag atcgcttctg
     2461 caatcgcgtc ctgttttttg acgtcgaacg cgtcgtctgc attcggcaac tcagccacgg
     2521 acggaaggta gcatgtgtgc tagaggaggc ccgtgccgac gctcaaagtg accgacgatc
     2581 tcggacgggc gctgctgttt gcgcatccgc cgcagcgcat cgtgtcgttg gtgccgagcg
     2641 acacccatag cgtgatcgcg ctcggggcgg gggaccggct ggtcggccgc acgagctggt
     2701 gcgagcacgg tgacggcgtt ccgaccgtgg gcggcacgaa ggacgtcgac gtcgacgcgg
     2761 tgatggcgct cgagccgcat ctcgtgatcg caaaccagga agaaaacccg cgcaagccgc
     2821 tcgaagcgct cgccgagcgc ggtgtgcagg tgctggtttc gctgccacgg cgagtcgatc
     2881 agggaatcgc gcacctcgca cggctcgctc ggatcctggg cgtggcggaa gcgccggcgg
     2941 ttcgcgagct gatcaagcgc ggctacgagc ttccggctgc gccgcccacg cggcagcgtg
     3001 cgttcattcc gatctggatg gatccgctga tgacgctcaa cgcggatacg tttggatcgg
     3061 acgtgctcgc gcgggtcggc gtcgagaacg cattcggcga tcgcttgcgc ctgtatccgc
     3121 tcgccgctga tctcggcaag accgcgccgc aagatgccgg caaccgcgac gtgcgctatc
     3181 cgcgaatcgc gaaggaagag atccaggcgc gcggcgcgga tctgatcatc ctgcccgacg
     3241 agccgcacgc attctcggcg gacgatgagg ccgagctgca cgcagtgctg ccgaaggcgc
     3301 gggtcgtgcg cgtgaacggc aaggatctgt tctggtacgg cgcgtggacg atcgaggcga
     3361 ttccgcggct gtccgcgcag ctcaccaatt gagccggcgc tcgaggcccg cgatgaagcg
     3421 gtcgagcgcg ggcgggtact tcgggaagaa caacgacggc tcgacgagct cgagctccgc
     3481 caccatcggc tgttcgtcgg gatcgcggac catgtcgacg cgcccgtaca ggatcctgtc
     3541 gacgtacggc gcgattgcgg cggtggcgac cgcgcgctcg ttgtcggcga tcgggaacgg
     3601 cccctcgatg cgttcgatgt cgccggcgaa ccgtggcgtc ttgcggatcg cgtgcgtgaa
     3661 ttcgccgtcg atccagatca gcgagcgttc gccgtaatcg tcgacggagg cgacatacgg
     3721 ctgcacgagt gcggcgccga gcgacgtcac gaatcgtagg tgatcgatgc cgttgcggcc
     3781 gtcggcgaag ctcttcgtgt gcaacgagcc ggcgccgatc tccggcttga tcacgatgcg
     3841 tgtggcaggg agctcgcaga tctcgccgcg ctcgaccagc ttcgtcggta ccacgggcac
     3901 gccgcgcgcc tgcatctcga gcaagtaacg cttgcgaacg ttggcccgga cgatatccgg
     3961 cgggttcaac agcggcgccg cagcggctgc gcggtcgacc cacgcgagga acgcgtcgag
     4021 ctgcagtgcg tagttccacg tcgagcggat gatcgtcggg atcggtgcat cccagtcggc
     4081 gctcgggtca tcccacgcga caagcggtgc gtcgatcccg gcggccgcaa gcgcggcgga
     4141 aagcggtgcc tcgtcggcgt cttcttcggg aagcgtcttg caagtcgcga cgcgaagctt
     4201 cacagcgcct gcaccagatc tt
//
LOCUS       DASUFX010000494         5525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_496409,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000494 DASUFX010000000
VERSION     DASUFX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496409"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFV99_19815"
     CDS             complement(<1..533)
                     /locus_tag="VFV99_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061630.1"
                     /translation="MRKHAALLGALLLAVLPACPSHPRAPRVAPAADVTVPIEGAIRG
                     VAATSGLQFAAVEANPSDHASALRAFRGVAEAWHGDIAGSAGPIAATDKLVVATSVVG
                     AGSQLDGAAVRGEPAAAIRAFDATSGAVKWTRVFDSTEWALATSIAVAGDGVIVGGTF
                     GGTLRAGKHVVSSAGGS"
     gene            complement(545..781)
                     /gene="rpmB"
                     /locus_tag="VFV99_19820"
     CDS             complement(545..781)
                     /gene="rpmB"
                     /locus_tag="VFV99_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5061631.1"
                     /translation="MSRVCQVTGKSPLVGSNVSHSNRHTKRRFEINLKTKRFWLEDEK
                     RWITLRVSARGMRIIDKLGLPAVVAQLRERGEKF"
     gene            complement(792..965)
                     /gene="rpmG"
                     /locus_tag="VFV99_19825"
     CDS             complement(792..965)
                     /gene="rpmG"
                     /locus_tag="VFV99_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5061632.1"
                     /translation="MAKKGKSGRELIRLVSTADTGFFYTTSKNRKRTPDKLVLKKYDP
                     VVRKHVEFKESKI"
     gene            complement(1020..2822)
                     /locus_tag="VFV99_19830"
     CDS             complement(1020..2822)
                     /locus_tag="VFV99_19830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5061633.1"
                     /translation="MPSLVFLAGPIAGRRYKLGDGEYVIGRRSDCQIFVPDMRVSRQH
                     ARLWRDGEGWSLEDLGSNNGTFINGAKLQTATPLRHDDEIMIANNKIRVEAQDAPSEQ
                     MPAEGRVVTIVDVGGQSLVRSREDSSAGRAALVSSGLMSVSDQKAVRLIERKLDAVTQ
                     ILQAIAATDTEESLLEKIVEALLELFPQAENVGVVVSDERTSELRVQCQRHRQREKVD
                     PSTTGPRPLTPELRIPGTIIQHVVSDRRGVLLGETTDGSNELIGTRMGAPLIYHGAHY
                     GVVYVESLKQSFKQEDVDLLQAIATQAGLAMHATRVAAQLARREKLERDLRVARQIQR
                     SLLPANVPQVVGLDFAVHYEPAYQIGGDFYDFIWHDPAHLGLVVGDVAGKAISAALYM
                     ARVTSELRSRAAIARTPARLLRRVNTEIARLGDDGMFATLVYCIYDLESRSLVFTNAG
                     HCVPLLRRGDRVFPLQAERAHTPPLGVTPDLDAGEARVQLHSGDMLIMVSDGILEARD
                     ARGVEYGLSRLSRRIRTARGGADDVVKAILADIDSHSQDQAQGDDMTIVAMAIEQRRA
                     KRKTTTIPGVLPPEAAESTRLASEGGTPAEDTDD"
     gene            2939..>5525
                     /locus_tag="VFV99_19835"
     CDS             2939..>5525
                     /locus_tag="VFV99_19835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5061634.1"
                     /translation="MQALGNYIGGVFVPPSGTALVSRNPAADGAVVFETGFTTSAVSD
                     AAAAGATAQPVWAALTLAERFAHLERFKAEIAARAGELADAIVIETGKIRSEAKTEIQ
                     TLLNRFDLVKNAMAADLKAGIVAPGESLRYQPLGVVGVIGPFNFPLHLCHAHVVPALL
                     AGNTVVIKPSDITPLCGQRYAEAAHAAKLPPGVLNVVVGDGTVGAAMVQAKELRGLCF
                     TGSWTVGRRILEAAVERPELLVALEMGGKNVCVVLDDAALRQSVHEVAVGGYLSAGQR
                     CTGTERVLVHRKIADRFIDALARCVKTLRFGNPDDASVFAGPLATHGALTKVEHAIEV
                     ARKAGCEPIVPGEKLPGGSYRTASLHRVPDGQHHIAGYTDFEVFGPDLSVEVIDSDEE
                     AIAAINESPYGFVNAVFTESQKRFEQFATRTKSGMLNRNRSTNLASPKLPFGGVGHSG
                     NYRPAGAWAHRNVTAPLAMLENVIGAVTPHPHLAAHLPGVDLDRLERQHTAEEVAETK
                     RGLIDTPRPMSLIRPRGGVLPDSAALLARLYAGDRVPKEKKPPVFDHLRSAGPWMVSV
                     DAEPLSVLDGMSQTATVVGGFAEDPVVRAFTEGEFADTLVTNDDTAVRETWSAIEYAN
                     VLRQLVPGLPHVTFVASGAEANEKAMALCRINRPNATKVLAFEGSFHGRTWLALHATH
                     SPSKRKPFELDGYQCTFAPFPVWETPGDEPTAPPGFYAAAANGELDELKERYGDAKDD
                     RLLAAEVASLAAVHDAIKGGDYACVIIEPMQSEGGDRYATERFFRALRLLTRRHQTFL
                     VFDEVQVGFGLGGPFAWHSKFRLLSARGQQDYPDAVTFAKRAQVGVVMSRFADPEPAS
                     AHNASL"
BASE COUNT          859 a         1895 c         1851 g          920 t
ORIGIN      
        1 tcgctgccgc ccgcgctcga cacgacatgc tttcctgcgc gcagcgtgcc gccgaacgtg
       61 ccgccgacga tgacgccgtc accggcgacg gcgatcgatg tcgcgagtgc ccactcggtc
      121 gagtcgaaca cccgcgtcca cttcaccgcg ccgctcgtcg catcgaacgc gcggatcgcc
      181 gcggccggct caccgcgaac ggcggcacca tcgagctgtg agccggcgcc gacgaccgag
      241 gtcgcgacga cgagcttgtc ggtcgcggcg atcgggccgg cactgcccgc gatgtcgccg
      301 tgccacgcct cggcaacgcc gcggaacgcg cgcaacgcgg atgcgtggtc gctcgggttg
      361 gcttcgaccg cggcgaattg caggccgcta gtcgcggcaa caccgcgaat cgcgccctcg
      421 atcggcacgg tcacatccgc ggccggcgca acgcgcggcg cacgcgggtg acttggacac
      481 gccggcagca ctgcaagcag cagcgcgccg agcagagcag cgtgcttacg cacgcgcccc
      541 ttgcttagaa cttctcgccg cgctcgcgca actgcgcgac gaccgcgggc agacccagct
      601 tatcgatgat gcgcattccg cgcgcggaca cgcggagcgt gatccagcgc ttctcgtctt
      661 cgagccagaa gcgcttggtc ttcaggttga tctcgaagcg acgcttggtg tggcggttcg
      721 agtggctaac gttcgagccg acgagcggcg acttcccggt gacttgacac acgcgcgaca
      781 tggcacgtct cctagatctt cgactctttg aattcgacgt gcttgcggac gacggggtcg
      841 tacttcttca gcacgagctt gtcgggcgta cgcttgcggt tcttcgaggt cgtgtagaaa
      901 aagccggtgt ccgccgtcga aacgaggcgg atcaactctc ggcctgactt gccttttttc
      961 gccatagcac taactccggg gctgggactt atggcatgga ctcccgcggt ttggcaaggc
     1021 tagtcgtccg tgtcctcggc gggcgtcccg ccctcgctgg caagccgggt cgactcggcg
     1081 gcctcgggcg gtaaaacccc tggaattgtc gtggtcttac gcttggcccg gcgctgctcg
     1141 atcgccatcg cgacgatcgt catgtcgtcg ccctgggcct gatcctggga gtggctatcg
     1201 atgtccgcga ggatggcttt gaccacgtcg tcggccccgc cgcgggccgt gcggatccgg
     1261 cgggacagcc gcgacaggcc gtactcgacc ccgcgagcat cgcgggcttc gaggatgccg
     1321 tccgacacca tgatcagcat gtcgccggag tgcagctgca cgcgggcttc gccggcatcg
     1381 aggtcgggcg tgacaccgag cggcggcgtg tgcgcgcgtt cggcctgcag cggaaacaca
     1441 cggtcgccgc ggcggagcaa cggcacgcag tggcccgcgt tcgtgaacac gaggctgcgc
     1501 gactcgagat catagatgca gtagacgagc gtcgcgaaca tgccgtcatc gccgagcctc
     1561 gcgatctcgg tgttgacgcg gcggagcaaa cgcgcgggcg tgcgtgcgat cgcagcgcgc
     1621 gacctgagct cgctcgtcac gcgcgccatg tagagcgccg cgctgatcgc cttgcccgcg
     1681 acatcgccga cgacgagccc gaggtgcgct gggtcgtgcc agatgaagtc gtagaagtcg
     1741 ccgccgatct gatacgcggg ctcgtagtgc acggcgaagt cgaggccgac cacctgcggc
     1801 acgttcgccg gtagcagcga gcgttggatc tggcgagcga ctcgtagatc gcgctcgagc
     1861 ttctcgcggc gtgcgagctg cgcggcgacg cgcgtcgcgt gcatcgcgag cccggcttgc
     1921 gtcgcgattg cctgcaatag atcgacgtct tcctgcttga acgactgctt caagctctcg
     1981 acgtaaacca cgccgtaatg cgcaccgtga tagatgagcg gcgcgcccat ccgcgtgccg
     2041 atcagctcgt tggagccgtc cgttgtttca ccgagcagca cgccgcggcg atcgctgacg
     2101 acgtgctgga tgatcgtgcc cgggatgcgc agctcgggcg tgagcggacg cggaccggtc
     2161 gtgctcgggt cgaccttctc gcgctggcga tgacgctggc actgcacccg tagctcgctc
     2221 gtgcgttcgt ccgagacgac gacaccgacg ttctccgcct gcgggaacag ctcgagcagc
     2281 gcctcgacga tcttttcgag cagcgactcc tcggtatcgg tcgccgcgat cgcctgcagg
     2341 atctgggtga cagcgtcgag cttgcgctcg atcaggcgca ccgccttctg atcgctgacg
     2401 ctcatcaggc ccgagctgac gagcgctgcg cgaccggcgc tcgagtcttc gcgcgagcgc
     2461 accagcgatt gcccaccgac gtcgacgatc gtgacgactc ggccttcggc cggcatctgc
     2521 tcgctcggtg cgtcctgcgc ctcgacgcga atcttgttgt tcgcgatcat gatctcgtcg
     2581 tcgtgacgga gcggcgtcgc ggtctgcagc ttggcgccgt tgatgaacgt gccgttgttg
     2641 ctgccgaggt cttcgagcga ccagccttcg ccgtcacgcc acagccgcgc gtgctgccgg
     2701 ctgacccgca tatcgggcac gaaaatctgg cagtccgagc ggcggccgat gacgtactcg
     2761 ccgtcgccca gcttgtagcg gcggcccgcg atcggcccgg ccagaaacac cagtgacggc
     2821 atgcctaggt tcgctagata ccacggcttt tactgagcgg cgcccacccc ttcagacagt
     2881 gctccgtgag gcgttgggcc agggcgcgac cactggggtt tcgtggtcta tttagcccgt
     2941 gcaggctctg ggtaattaca tcggtggcgt atttgtcccg ccatcgggca ccgctctcgt
     3001 gtcgcgcaac cccgcggccg atggcgcggt tgtgttcgag accggtttca ctacatccgc
     3061 agtgagcgat gccgcagcag cgggcgctac cgcgcagccc gtatgggccg ccctcacgct
     3121 tgccgaacgc ttcgcgcacc tcgaacgttt caaggccgag atcgccgcgc gcgcaggaga
     3181 gctcgccgac gcgatcgtga tcgagaccgg aaaaattcgc agcgaagcga agaccgagat
     3241 ccagacgctg ctcaatcgct tcgacctcgt gaagaacgcg atggccgctg acctcaaggc
     3301 cggcatcgtc gcgcccggtg agtcgctgcg ctatcagccg ctcggcgtcg tcggcgtgat
     3361 cggcccgttc aacttcccgc ttcacttgtg tcacgcgcac gtcgtccccg cgctgctcgc
     3421 gggcaacacg gtcgtgatca agccgagcga catcacgccg ctgtgcggtc agcgctacgc
     3481 cgaagccgcg cacgctgcga agctgccgcc cggcgtgctc aacgtcgtcg tcggtgacgg
     3541 cacggtcggc gccgcgatgg tgcaggccaa ggagctgcgc ggcctctgct tcaccggcag
     3601 ctggaccgtc ggccggcgca tcctggaagc ggccgtcgaa cgcccggagc tcctggtcgc
     3661 gctcgagatg ggcggcaaga acgtgtgcgt agtgctcgac gacgcggcgc tgcgccagtc
     3721 cgtgcacgaa gtcgccgtcg gcggttatct gtctgcaggc cagcgctgca ccggcaccga
     3781 gcgcgtgctc gtgcatcgca agatcgccga tcgcttcatc gacgcgctcg cacgctgtgt
     3841 gaagacgctg cgcttcggta accccgacga cgcgagcgtg tttgccggac cgctcgccac
     3901 gcacggcgcg ctcacgaagg tcgagcacgc gatcgaagtc gcgcgcaaag ccggctgcga
     3961 gccgatcgtg cccggcgaga agctgccggg tggctcgtac cgcacggcgt cgctgcatcg
     4021 cgtgcccgac ggccagcacc acatcgcggg ctacaccgac ttcgaggtgt tcggtcccga
     4081 tctgtcggtc gaggtgatcg actccgacga ggaagccatc gccgcaatca acgaaagccc
     4141 atacggcttc gtcaacgcgg tgttcaccga gtcgcagaag cggttcgagc agttcgcgac
     4201 gcgcacgaag tccggcatgc tcaaccgcaa ccgctcgacg aacctcgcga gcccgaagct
     4261 gccgttcggc ggcgtcggtc acagcggcaa ctaccgtccc gccggtgcgt gggcccatcg
     4321 caacgtcacc gcaccgctcg cgatgctcga gaacgtgatc ggcgcggtca cgccgcatcc
     4381 gcatctcgcc gcgcatctgc cgggcgtcga tctcgaccgc ctggagcgcc agcacaccgc
     4441 cgaggaagtc gcggaaacga agcgcggcct catcgacacg ccgcggccga tgtcgctgat
     4501 ccgtccgcgc ggtggcgtac tgcccgacag cgcggcgttg ctcgcgcggc tctacgccgg
     4561 cgatcgggta ccgaaggaga agaagccgcc ggtgttcgat cacctgcgct cggcgggacc
     4621 gtggatggtc tccgtcgatg ccgagccgct cagcgtgctc gacggcatga gccagaccgc
     4681 gacggtcgtc ggtggcttcg ccgaggatcc ggtcgtccgc gcgttcaccg aaggcgagtt
     4741 cgccgacacg ctcgtcacga acgacgacac ggcggtgcgc gagacctggt ccgcgatcga
     4801 gtacgcgaac gtgctgcgcc agctcgtgcc tggtttgccg cacgtcacgt tcgtcgcatc
     4861 gggcgccgag gcgaacgaga aggcgatggc gctgtgccgc atcaaccgcc cgaacgcgac
     4921 gaaggtcctc gcgttcgagg gcagcttcca cggccgcacg tggctcgcgc tgcacgcgac
     4981 gcactcgccg agcaagcgca agccattcga gctcgatggc tatcagtgca cgttcgcgcc
     5041 attcccggtg tgggagacgc ctggcgacga accgaccgca ccgccgggct tctacgccgc
     5101 cgcggcgaac ggcgagctcg acgagctcaa ggagcgctac ggcgatgcga aggacgatcg
     5161 gctgctcgcg gccgaggtcg cgtcgctcgc ggctgttcac gatgcgatca agggcggcga
     5221 ctacgcgtgc gtgatcatcg agccgatgca gtcggaaggc ggcgatcgct atgcgaccga
     5281 gcgcttcttc cgcgcgctgc gcctcctgac gcgccgccat cagacgttcc tcgtgttcga
     5341 cgaggtgcag gtcggcttcg gcctcggcgg tcccttcgcg tggcactcga agttccgcct
     5401 gctttcggcg cgcggccagc aggactatcc cgacgcggtc acgttcgcga agcgtgcgca
     5461 ggttggcgtc gtgatgtctc ggttcgcaga ccccgagccg gcgagcgcgc acaacgcctc
     5521 gctcg
//
LOCUS       DASUFX010000495        13406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_633518,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000495 DASUFX010000000
VERSION     DASUFX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633518"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFV99_19840"
     rRNA            <1..414
                     /locus_tag="VFV99_19840"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            703..819
                     /gene="rrf"
                     /locus_tag="VFV99_19845"
     rRNA            703..819
                     /gene="rrf"
                     /locus_tag="VFV99_19845"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            927..2033
                     /locus_tag="VFV99_19850"
     CDS             927..2033
                     /locus_tag="VFV99_19850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5061635.1"
                     /translation="MRKLLVVGAAVALAIGCKKKEEKKEAPPPPPPVTATADAAAEQP
                     PGPDPKVVERGAYLAKAGACLVCHTAMGPQGPDLANVGAGGLEMKEKFGTWRSPNITP
                     DKGTGIGNWTDDQIAAAIREGIRPDGTKLTPIMPYMNFNGMTDDDVKAVVTFLRTLKP
                     VEKQIAKFELKMPPINAPKPANLPPGDDPVKKGQYMASIMLCNHCHWTPNKDMTGPVG
                     PDHMFSGGLPMEMPPLGTGTLYTANITSDPDTGIGKWTEEQIFTALKTMTKPDGKMIQ
                     GPMLFMQGMWSQLNDDDMKNVATFIKSLPPVKNKVPKSTFKPNPGPPGGGPPGAGGPP
                     GAGGPPGAGGGSAAGSGAGSAAKPAADKDSAAKK"
     gene            complement(2510..3640)
                     /locus_tag="VFV99_19855"
     CDS             complement(2510..3640)
                     /locus_tag="VFV99_19855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5061636.1"
                     /translation="MLALLVLGLFVLVIIAGALPRLRNRERVRRETRDLAIPFVTIVR
                     PDRSAPAQDIVLPGNVEAFANAPIFARANGYLRKWHVDIGAHVSKDQLLAEIDVPELT
                     QQLQQARSTLAASEAQLRVAEATAKRYTELRQTHAVSQQEVDTAVGALQTNQGTVAAN
                     QASVRQLEQQVAFAQVRAPFAGIITARNVDVGDLINAGSSTAAGTALFQIAQPNKLRV
                     YTSVPERSATAVTPGMAATLSLAAFPDRRVAGTVVRSANAIDPTTRTLLVEIEVPNPT
                     KTLFSGTFAEVRLAIPTTQGVFTVPVEALLFRNEGLQVATVVGDRAVMKSIVPLRDFG
                     DRIEVGSGLTGDELVILNPPDSIANGSPVRVAPPSMPILSKR"
     gene            complement(4120..7365)
                     /locus_tag="VFV99_19860"
     CDS             complement(4120..7365)
                     /locus_tag="VFV99_19860"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5061637.1"
                     /translation="MWIVRLALRRPYTFVVVAILIVMFGVLSILRTPKDVFPRIDIPV
                     VSIVWSFAGLDAEEMANRIVTITERALTTTVDNIQHIESQSLDGVAVVKLFLHERANV
                     IQAIAQVTAVSQTQLRALPTGTTPPLIITYTASTVPVLQLGLSGNGLSEQQLFDLAIN
                     FIRTRLITVSGAAIPYPYGGRFPQVMVDLNPAALQAKGLSPIDVVNAINAQNLILPAG
                     TVKIGELEYNVDLNGSPTTVDEINNLPIKTIGNTTIYIRDVAWVRFGYPPQINVVRVD
                     GQRSVLITIQKAGNASTLDVVDGVKAMLPSVLAGLPPNLQVTPLADQSLFVRGAVSGV
                     VREGVIAACLTAFMILVFLGNWRSTLIIAISIPLAILTSIIALSALGETINVMTLGGL
                     ALAVGILVDDATVEIENINRQAGLGKPILQAILDGAAQIAVPAFVSTLSICIVFVPMF
                     FLTGVARYLFIPLAEAVVFAMLASYLLSRTLVPTMARYLLRGHEHECGHAPRRKPNVF
                     VRVQRRFEHHFENLRGRYRGVLMWCLGSRALFLLAVLGFCLASIVAIVPVLGQDFFPA
                     IDSGQFRLHVRARTGTRIEDTARLCDQIEKVIRERIPPGEVATIIDNIGLPVSGINLT
                     YSTSAPVGPADADILVSLKRGHQPTAEHVHDLRIALAREFPGTTFSFTPADIVGQILN
                     FGQPSPIDIQIGGRDVDASRRFATMLLPELRQVPGLVDTRIQQPDDRQGLFIAVDRTK
                     ASQVGLTQRDVATNLLTALSGSSQVSPTFWLNPKNGVSYSIATQAPQYRLSSLALLQN
                     LPIVGTAGRPPQILGNLSTITRRAGAPVVSHYDVTPVIDIFAAVQGRDLGGVARDVER
                     IIDAHRHELPKGTQVVMRGQVETMRTSYVGLLAGLAFAIVLVYLLMVVNVQSWLEPFI
                     IITALPAALAGIAWMLFVTQTTLSVPALMGAIMCMGVATANSILVVTFASEQLRAGKD
                     ARTAVLEAGYTRFRPVIMTALAMIIGMVPMAFGLGEGGEQNAPLGRAVIGGLLLATVS
                     TLLFVPTLFSVIYERMSRPKPHAAGAAHAADRADGHEPIPPNRAPPSGGAT"
     gene            complement(7375..8841)
                     /locus_tag="VFV99_19865"
     CDS             complement(7375..8841)
                     /locus_tag="VFV99_19865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX5061638.1"
                     /translation="MKRRSLCAWALVPQIALGACVVGPSYQVPPAPTPTSARYKEAGT
                     WKVASPGDALPRGPWWTMFRQPELDALQAHLNVNNQTIAAAFENYLAARALIRAAEAQ
                     YYPTIVFQPSTSRGRSPGVTGANPSAVTGSSATTGGGIPGPFEAYVLAGQGSWVPDLF
                     GRVRNNVRGRRYNAQASAADLESTRLAAQASLTATYFQLRGQDALIELLDKTVTANAE
                     IASITRTRYEAGLENESVVVQAELTLQAARVQATNAGVLRGEYEHAIAALIGVPPTDF
                     SMPHRALLATPPRIPLGAPSHLLERRPDIASAERQMAAANAAVGVGYAAYFPVITLSG
                     TAGFATSTLSTLFHWPSRLWSTGLSLAQTIFDGGERRANLDQLYALYFATVAKYRQTV
                     LTAFQQVEDNLTQTRILVDVIEQQRTAVALAQRGFDLQRVRYETGLDPYINLMVQQTA
                     LLSAQQQLVSFQVQQMLATVQLVQALGGGWDRSLLPKE"
     gene            9029..9364
                     /locus_tag="VFV99_19870"
     CDS             9029..9364
                     /locus_tag="VFV99_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061639.1"
                     /translation="MKSFVILCALFVTACGGHGEGEIDDVVGAACMNDRDCDSRCYFG
                     GDFPGGFCSLPCASDNDCPGDTYCMREGGGVCMFACPAFDCTRLGQGWECRDQERQNG
                     GHVNVCSGG"
     gene            complement(9361..10638)
                     /gene="tyrS"
                     /locus_tag="VFV99_19875"
     CDS             complement(9361..10638)
                     /gene="tyrS"
                     /locus_tag="VFV99_19875"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5061640.1"
                     /translation="MSIVDELEARGLYANCTDRAGLSELLAKEAPVSAYAGYDPTAPS
                     LHVGNLVPTILLKRLQLAGHRPIIVVGGATGMIGDPSGKSDERNLLDDATLATNLAGI
                     RSELGRLLDFDDKRTGALMTNNADWTRGVTFLEFLRDVGKYLTINYMMGKDSVKSRIE
                     SENGISYTEFSYMLLQAFDFVELNKHHNCRLQVGGNDQYGNITAGTELQHKLGRPKIF
                     GLTAPLLLDSTGQKMGKTSTGERIWLSPERTPPFEFYKYWFNVSDADAPRFLRMFSLR
                     PLAELDELLLAHDADRAKRMAQRELARAMTTWVHGEAQVTRVEWANGVFATGTLDGLS
                     STDLTKLAEIIPTIDIARTELGDGIGIVDLIARTVADSKSTARTKITQGGAYVNNVRI
                     DDVKHKVTLEQFADQRLLLVRGGKKDYRLVRVV"
     gene            complement(10666..11580)
                     /locus_tag="VFV99_19880"
     CDS             complement(10666..11580)
                     /locus_tag="VFV99_19880"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5061641.1"
                     /translation="MRIDTVITDVRVFPLLQVSKEDLEEAAGGPLKTIEPVNGGLTNT
                     IHKVTTESGDVFGVKHYAGGKDWFDTELTTLTLLHGTLPVPDVVHADEKRLVIVYRWI
                     DGITLHDLRKQGEHTAFATLAEPLGRVLAWLAKSDATEPFELTPILEQAYGQLTTGRA
                     RARMGAPMADGLRRAMEAAEPAMAFGSVCLSHGDLGHRNVLVYQAGDRWRINGLIDWE
                     TTTTGSPLVDIGSLFRYSKRYDRAFCEAFARGYTDAGGRLPDNWLLTSRLLDATWLVD
                     MLADEHEHPAVFADCRKLVMNLLAEAAP"
     gene            complement(11596..13170)
                     /gene="rny"
                     /locus_tag="VFV99_19885"
     CDS             complement(11596..13170)
                     /gene="rny"
                     /locus_tag="VFV99_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5061642.1"
                     /translation="MEPVFILVAVVIGLLAGVFLGTRLGRARGDSGAAKVAEEEAEKI
                     RTAANAEVEAIKQAAEVEGKEAARKRKAELEDDLKQRKAELQKREESIAQKERDIDKQ
                     RKDAERRAGELDKKEKSVEGKIKQADAAVEKAESAQADARKKLEQIAGLSEEAARKKL
                     EEEVRGQALASAAVEIKRIEDDAAKEATARAKTIVATAIQRFASEFVHERTVAVIPLP
                     SDDLKGRLIGREGRNIRALEAATGIDMIIDDTAEAITISCFNPVRREVARIAISALVA
                     DGRIHPTRIEECVRKAEKEVDKVCKEAGEQAVFDLGLHRVHPELVRHLGRLKFRQSYA
                     QNVLLHSIEVGYLAGLIASELGANVKLARRAGLLHDVGKAIDHEQEGPHSEVGAALCR
                     KHGESPKVCQAIASHHGDEGGPKPQALLDHIVAAANALSAARPGARREQLASYIKRLD
                     DLEAICKRFGGVERAYALQAGREIRVMVMNDQVDDAQAIVMSKEIARAIETEATYPGQ
                     VRVVVVRETRASDYAR"
     gene            complement(13170..>13406)
                     /locus_tag="VFV99_19890"
     CDS             complement(13170..>13406)
                     /locus_tag="VFV99_19890"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5061643.1"
                     /translation="DLSEIAAVFVPGLAFDRAGGRIGWGRGYYDVTLAAASPAALRIG
                     LAFECQIIEHVPREPHDAALHYVVTEAAIYRAPD"
BASE COUNT         2235 a         4240 c         4563 g         2368 t
ORIGIN      
        1 ttggcacctc gatgtcggct catcgcatcc tggggctgaa atcggtccca agggtttagc
       61 tgttcgctaa ttaaagcggt acgcgagctg ggttcagaac gtcgtgagac agttcggtcc
      121 ctatccgtcg tgggcgcagg atacttgaga ggagctgact ttagtacgag aggaccgagt
      181 tggacggacc gctagtgttc cggttgtggc gccagccgca tcgccgggta gctatgtccg
      241 gaagggataa ccgctgaaag catctaagcg ggaagccccc ctcgagatta ggtatcccaa
      301 atgccgtcaa ggcaccataa gaccccgcgt agactacgcg gttgataggc gcggtgtgga
      361 aatgctgcaa agcatggagc ttacgcgtac taatcggtcg ggcggcttga ccacctatat
      421 ttccgtcgct gcatgcgcaa gcaggcagtg acaccagcga tcgggatcat ttgattccgg
      481 tcactggtcg gcgccaacac agctcaccac gggctgtgct ggaggctcgc cgggagacgg
      541 tggaagccat caccctagtt cgccagttcc aagatgactt gttcatctcg gactgcgcga
      601 tgtagacgag caatcagata cgactcaatc gctgctcacc agagcaacta catgaccgcc
      661 ggacaccatg ccggccgggc ttcgcacttc gtcgattttt ttcctggtgg caacaccgga
      721 gaggtcacac ccgttcccat cccgaacacg gaagttaagc tctccagggc cgatggtact
      781 gcaggcgcaa gtttgtggga gagtaggacg ctgccggggt tttgagggcc cgctcatcga
      841 gcgggccctc atctttttcg gcgtgcgcgc tttttcggca gcggcgtttg cgtcgatgta
      901 ggccgtcatg cgaaccatgg cggaacatga gaaaactgct tgtcgtgggg gcagcggtag
      961 cgctggctat tggctgcaag aagaaggaag agaagaagga agcaccgccg ccaccgccgc
     1021 cggtcaccgc gaccgcggat gccgcggccg agcagccgcc cggacccgat ccgaaggtcg
     1081 tcgagcgtgg cgcctacctc gcaaaggccg gcgcctgcct ggtctgccac acggccatgg
     1141 gaccgcaggg acccgacctc gccaatgtcg gcgcgggcgg gctcgagatg aaagagaagt
     1201 tcggcacctg gcggtcgcca aacatcacgc ccgataaagg caccggcatc ggcaactgga
     1261 ccgacgacca gatcgccgcg gcgatccgcg aaggcattcg ccccgacggg acgaagctga
     1321 cgccgatcat gccgtacatg aacttcaacg gcatgaccga cgacgacgtc aaagccgtcg
     1381 tcacgttcct gcgcacgctg aagcccgtcg agaagcagat cgcgaagttc gagctcaaga
     1441 tgccgccgat caacgcaccg aagccggcga acctgccgcc cggcgacgac ccggtcaaga
     1501 agggtcagta catggcgtcg atcatgctct gcaaccactg ccattggacg ccgaacaagg
     1561 acatgaccgg tccggtcggc cccgatcaca tgttctcggg tggtcttccg atggaaatgc
     1621 cgccgctcgg tacgggcacc ctctacaccg cgaacatcac gtccgatccc gacaccggca
     1681 tcggcaagtg gaccgaggag cagatcttca cggcgctcaa gacgatgacg aagccggacg
     1741 gcaagatgat ccagggtccg atgctgttca tgcagggcat gtggagtcag ctgaacgacg
     1801 acgacatgaa gaacgtcgcc acgttcatca agagcctgcc gccggtgaag aacaaggtgc
     1861 cgaagtcgac cttcaagccg aacccaggcc cgccgggtgg cggtcctccg ggcgctggcg
     1921 gtcctccggg tgctggcggt cctccgggtg cgggcggtgg ctcggccgca ggctcgggcg
     1981 cgggctcggc agcgaagcca gccgccgata aggacagcgc cgcgaagaag tgatccccgc
     2041 gaagcgcgtg cagtgagccg cgcgacgtga cgaacgagcg atcgatcccg gtcgctcttt
     2101 tcgttttcgg cagcgacgca cgaatgaccg atctcgccag cctgttcgtc cggctcaagt
     2161 cggtacagcc gttcaatacc gatacggggt ttcttttgac gcgcgtcaag ttccggtggc
     2221 cgctcgagtc cggtcccgtc cggtacgaac cgattagcgg acgggctgtc cgggtccgca
     2281 ccggaacgct ggctgaaacg accgcgggtc gagccggacg gaagcgaggc tctcgtttcg
     2341 acacgatcgg catcgacggc gtcgcgacgc ggaccgaaga gccgttcggg tatcgcttcg
     2401 acacgatcgg catcgacggc gtcgcgacgc ggaccggtga gccgttcggg tatcgcttcg
     2461 acaggatcgg catcgacggc ggcgcgacgc ggaccggcga gccgttcggc tatcgcttcg
     2521 acaggatcgg catcgacggc ggcgcgacgc ggaccggtga gccgttcgcg atcgagtccg
     2581 gcgggttcag gatgacgagc tcgtcgccgg tcaggccact gccgacctcg atgcggtcgc
     2641 cgaagtcgcg cagcggaacg atcgacttca ttactgcgcg gtcgcccacc accgtggcga
     2701 cctgcaggcc ctcgttgcga aatagcagcg cttcgaccgg caccgtgaag actccctgcg
     2761 tcgtcgggat cgcgagccgg acctcggcaa aggtgcccga gaacagcgtc ttcgtcgggt
     2821 tcgggacctc gatctcgacc agtagcgtcc gcgtcgtcgg gtcgatggca ttcgccgagc
     2881 gcacgaccgt gccggcgacg cgacggtcgg ggaacgcggc gaggctcagc gttgccgcca
     2941 tgccaggcgt gaccgccgtc gccgaccgct cgggtaccga cgtgtagacg cgtagcttgt
     3001 ttggctgtgc gatctgaaac agcgcggtac cggccgccgt gctgctgccg gcgttgatca
     3061 gatcgccgac gtcgacgttg cgcgcggtga tgatgccggc gaacggggcg cgtacttgcg
     3121 cgaacgcgac ctgctgctcg agctggcgaa cgctcgcctg attggcggcg accgttcctt
     3181 gattcgtctg caacgcgccg acggcagtgt cgacctcttg ctgcgagacc gcatgcgtct
     3241 ggcgcagctc cgtgtagcgc ttcgccgtcg cttccgcgac gcggagctgg gcttctgacg
     3301 cggctagggt tgagcgcgct tgctgcagct gctgggtgag ctcgggcacg tcgatctcgg
     3361 cgagcagctg gtccttggac acgtgggcgc cgatgtcgac gtgccacttg cgcagatagc
     3421 cattcgcacg cgcaaagatc ggagcgttcg cgaatgcctc gacgttgcca ggcagcacga
     3481 tgtcctgcgc cggggcgctg cgatcgggac ggacgatggt gacgaatgga atcgcgaggt
     3541 cacgcgtctc gcggcgaacg cgctcgcgat tgcgaagtcg cggaagcgca ccggcgatga
     3601 tcacgagcac gaacaggcca agcacgagta gcgcgagcaa cgggtgccgg gctcggtgcc
     3661 gccgccgcgg tgcgggtcgg tcggcagtcg gcggctccgg tgcgggtgga tcggcagtcg
     3721 ccggctgcgg cggcgacgcc gcgtcggcgg tcgactgcgg tgatggtgcg gccggatcgg
     3781 cggtcgaacg cgctggtgcg ccggccggat cggcggtcga atgcggtgct actgcggccg
     3841 gatcggcagt cgactgcggt gatgctgcgg ccggatcggc ggtcgactgc ggtgatgccg
     3901 cggccggagt ggtaatcggc gtctgcggcg gcgcggccgg atcggtggcc gaccgcggtg
     3961 ctgctctggg cggattggtg gtcggcggtt gcggtggtgt tgcagcggaa gtcgtggttg
     4021 accgcgatgt tgctccggcc ggatccgcgg tctgctccgg tgagccggcg gctggctccg
     4081 ggcacggctc gggcggcgcg ctcggtggtg ccttgtcggt caggttgcac ctccgcttgg
     4141 aggtgctcgg tttggaggta ttggctcgtg accgtcagcg cgatcggctg cgtgcgccgc
     4201 gccggcagcg tgcggtttgg ggcgcgacat ccgctcgtag atgacgctga acagcgtcgg
     4261 cacgaacaac agcgtcgaca ccgtcgcgag caagaggcca ccgatcaccg cgcgtccgag
     4321 cggcgcattc tgctcgccgc cttcaccgag gccgaacgcc atcggcacca tgccgatgat
     4381 catcgcgagc gcggtcatga tcaccggacg aaaccgtgtg tagccagcct cgaggacggc
     4441 cgtgcgggca tccttgcctg cgcgcagctg ctccgaggcg aacgtgacca cgaggatgct
     4501 gtttgccgtc gcgacgccca tgcacatgat cgcgcccatc agcgccggca cgctgagcgt
     4561 ggtctgcgtg acgaacagca tccacgcgat gccggcaagc gccgcaggca atgcggtgat
     4621 gatgatgaac ggctcgagcc acgactggac gttcaccacc atgagcaaat agacgaggac
     4681 gatcgcgaac gcgaggcccg cgagcagacc gacgtacgac gtgcgcatcg tctcgacctg
     4741 gccgcgcatc acgacctgcg tgcctttggg tagctcgtgg cgatgtgcat cgatgatgcg
     4801 ttcgacgtcg cgtgcgacac cgccgaggtc gcggccctgg accgccgcga agatatcgat
     4861 caccggtgtg acgtcgtagt gcgagacgac cggagcaccg gccctgcgtg tgatcgtgct
     4921 caggttgccg aggatctgtg gtgggcggcc cgccgtgccg acaatcggta ggttctgcaa
     4981 gagggcgagc gaggacaggc gatactgcgg cgcttgcgtc gcgatcgagt agctgacgcc
     5041 gttcttcggg ttgagccaga acgtcggcga cacttgcgag ctgccgctca gcgccgtgag
     5101 gaggttcgtc gcgacgtcgc gctgcgtgag cccgacctgg ctcgccttcg tgcgatcgac
     5161 cgcgatgaac agcccctggc gatcgtctgg ctgctggatg cgcgtgtcga cgaggccggg
     5221 gacctggcgg agctcgggca gcagcatggt ggcgaagcgg cgcgacgcgt cgacatcgcg
     5281 gccgccgatc tggatgtcga ttggtgacgg ctggccgaaa ttcaggatct ggccgacgat
     5341 gtctgccggc gtgaacgaga acgtcgtgcc cggaaactcg cgagccagcg cgatgcgcag
     5401 atcgtggacg tgctcggctg tcggctggtg accgcgcttg agcgagacga ggatgtcggc
     5461 gtccgccggg ccgaccggtg ccgacgtgct gtacgtgagg ttgatgccgc tgaccggcag
     5521 gccgatgttg tcgatgatcg tcgcgacctc gccgggcggt atccgctcgc ggatcacctt
     5581 ctcgatctga tcgcacagcc gcgcggtgtc ctcgatacgt gtgccggtcc gcgcgcgtac
     5641 gtggagccgg aattgaccgc tgtcgatcgc gggaaagaag tcctggccca gcacagggac
     5701 gatcgcgacg atcgacgcga ggcagaagcc gagcaccgcg agcaggaaca gcgcgcgaga
     5761 accgaggcac cacatcagta cgccgcgata gcgaccacgc aggttctcga agtgatgctc
     5821 gaaccggcgc tggacgcgga cgaacacgtt cggcttgcgt ctcggggcgt gaccgcactc
     5881 gtgctcgtgc ccgcgcaaaa ggtagcgcgc catcgtcggc acgagcgtac gcgacaacaa
     5941 gtatgacgcg agcatcgcga atacgaccgc ctcggcgagt gggatgaaca ggtagcgcgc
     6001 gacgccggtg aggaagaaca tcggcacgaa cacgatgcag atcgacagcg tcgacacgaa
     6061 cgccgggact gcgatctggg cagcgccgtc gaggattgct tgcaggatcg gtttgccgag
     6121 gccagcttgg cgattgatgt tctcgatctc gacggtcgcg tcgtcgacga gaatgccgac
     6181 cgcaagcgcg agaccgccga gcgtcatcac gttgatcgtc tcgccgagtg cgctcagcgc
     6241 gatgatcgag gtgaggatcg cgagcgggat cgagatcgcg atgatcagcg tgctgcgcca
     6301 gttgccgagg aacacgagga tcatgaacgc ggtgaggcag gctgcgatca cgccctcgcg
     6361 cacgacaccg ctgacagctc cgcgcacgaa cagcgactga tcggcgagcg gcgtgacctg
     6421 gaggtttgga ggcaacccgg cgaggacgct gggtagcatc gccttgacgc cgtcgacgac
     6481 atcgagcgtc gaggcgttac cggccttctg gatggtgatc agcaccgagc gctggccgtc
     6541 gacgcgcacg acattgatct gcggcggata gccgaagcgc acccacgcga catcgcggat
     6601 gtagatcgtc gtgttgccga tggtcttgat cggcaggttg ttgatctcgt cgacggttgt
     6661 cggcgagcca ttgagatcga cgttgtactc gagctcgccg atcttcacgg tgccggccgg
     6721 aaggatcaag ttctgcgcgt tgatcgcgtt gacgacgtcg atcggcgaca gtcccttggc
     6781 ctgcagcgcg gccgggttca ggtcgaccat cacctgcggg aaccgaccgc cgtaggggta
     6841 tggaatcgcg gcacccgaca ccgtgatgag ccgcgtgcga atgaagttga tcgcgaggtc
     6901 gaacagctgt tgctcggaca gtccgttgcc ggagaggccg agctggagca ccggcaccgt
     6961 cgacgcggtg tacgtgatga tcagcggcgg tgtcgtgccg gtcggcagcg cgcgcagctg
     7021 ggtctgcgaa acagcggtca cctgcgcgat cgcctggatc acgttcgcgc gctcgtggag
     7081 gaacagcttg accacggcga cgccgtcgag cgattgcgac tcgatgtgct ggatgttgtc
     7141 gacagtcgtc gtcagcgcgc gctccgtgat cgtgacgatc cggttcgcca tctcctcggc
     7201 gtcgaggcca gcgaagctcc acacgatgct gaccaccggg atgtcgatcc ggggaaagac
     7261 gtccttcggt gtgcgcagga tcgagagcac accgaacatc acgatcagga tcgcgacgac
     7321 gacgaacgtg taaggtcggc gcagggcgag gcggacgatc cacatcgcgc gctcttactc
     7381 ctttggcagc agcgagcgat cccagccgcc gccaagcgct tggacgagct gcacggtcgc
     7441 gagcatctgc tggacctgaa agctcacgag ctgctgctgc gctgacagca gagctgtctg
     7501 ctgaaccatg agattgatgt acggatcgag accggtctcg taacgcacgc gctggagatc
     7561 gaatccgcgc tgcgcgagcg cgacggcggt gcgctgttgc tcgatgacgt cgacgaggat
     7621 ccgcgtctgg gtcaagttgt cttcgacctg ctgaaacgcg gtgagcacgg tctgccggta
     7681 cttcgcgacg gtcgcgaagt agagcgcgta cagttggtcg aggtttgcgc ggcgctctcc
     7741 gccgtcgaag atggtctgcg ccaggctcaa gcccgtcgac cacagccgac tcggccaatg
     7801 gaacagcgtg ctcagcgtcg aggtcgcgaa gcccgccgtg cccgacagcg tgatcacggg
     7861 aaagtacgcc gcgtagccga caccgaccgc tgcgttggcc gccgccatct ggcgctccgc
     7921 agacgcgatg tcagggcggc gctcgagcag gtgcgacggc gcgccgagtg gaatgcgcgg
     7981 cggtgtcgcg agcaatgcac ggtgcggcat cgagaagtcc gtcggcggta cgccgatcag
     8041 cgccgcgatc gcatgctcgt actcgccgcg caacacacct gcgttcgtcg cctgcacgcg
     8101 agcggcctgc agcgtgagct ccgcttgcac gacgacggat tcgttctcga gcccagcctc
     8161 gtagcgcgtc cgtgtgatcg atgcgatttc tgcgttcgcc gtgaccgtct tgtcgaggag
     8221 ctcgatcagc gcgtcctgtc cacgcagctg gaagtacgtc gcggtgaggg atgcctgcgc
     8281 ggcaagccgc gtgctctcca ggtcggcggc gctcgcctgt gcgttgtagc ggcgcccccg
     8341 cacgttattg cgcacgcgcc cgaacaggtc cggcacccac gagccttggc cggcgagcac
     8401 gtaggcctcg aagggacccg ggattccgcc gccagtcgtc gcactcgacc cagtgaccgc
     8461 cgagggattc gcgccggtga cgccgggtga gcgcccgcgg ctcgtcgatg gttggaacac
     8521 gatcgtcggg tagtactgcg cctcggccgc acggatcagc gcgcgtgccg cgaggtagtt
     8581 ctcgaacgcg gcggcgatcg tctggttgtt gacgttcaag tgtgcttgca gcgcgtcgag
     8641 ctcgggctgt cgaaacatcg tccaccacgg accgcgcggc agtgcgtctc ccggcgaagc
     8701 gaccttccag gtgccggctt ctttgtatct cgctgatgtc ggcgtcggtg cgggcgggac
     8761 ttgatagctc gggccgacga cacacgcgcc cagcgcgatc tgcggtacga gcgcccacgc
     8821 gcagagcgag cgtcgcttca tgcgctgcta gcgatgcacg ctcgacgcca tccgcgggag
     8881 ggagtcagcg ccggtgtagt cgcaaaacgc ttgctagcgc cgttcgagac gcgcgcgatt
     8941 gttcgtgaat tgcacacagc gcgagcacga tcacgctgtg ttgcaccgcg ccttccgggc
     9001 acacgcagtg caccggtacc atctgcgtat gaagtccttc gtcatcttat gtgcgctgtt
     9061 cgtcacggct tgcggcggtc acggcgaggg tgaaatcgac gacgtcgtcg gcgcggcgtg
     9121 catgaacgat cgcgattgcg attcgcgttg ctatttcggc ggcgacttcc cgggcggctt
     9181 ctgcagcctg ccgtgcgcga gcgataacga ctgccccggc gatacgtact gcatgcgcga
     9241 gggcggtggc gtgtgcatgt tcgcgtgccc cgcgttcgac tgcacgcggc tcggccaagg
     9301 ctgggagtgc cgcgatcagg agcgccagaa cggcggccac gtcaacgtct gtagcggcgg
     9361 ttagacgacg cgaacgaggc ggtagtcctt cttgccgccg cgcacgagca gcagacgctg
     9421 atctgcgaac tgctcgagcg tgaccttgtg cttcacgtcg tcgatgcgga cgttgttcac
     9481 gtacgcgccg ccttgcgtga tcttggtgcg ggccgtgctc ttcgagtccg cgaccgtgcg
     9541 cgcgatcaga tcgacgatgc cgatgccgtc gccgagctcg gtgcgcgcga tgtcgatcgt
     9601 cgggatgatc tcggcgagct tcgtcaggtc ggtgctcgac aggccatcga gcgtgccggt
     9661 cgcgaacacg ccgttcgccc actcgacgcg cgtgacctgc gcctcgccat gcacccacgt
     9721 tgtcatcgcg cgagcgagct cgcgttgcgc catgcgcttt gcgcgatcgg catcgtgcgc
     9781 gagcagcagc tcgtcgagct ccgcgagcgg ccgcagcgag aacatgcgca gaaagcgcgg
     9841 cgcatcggcg tcgctgacgt tgaaccagta cttgtagaac tcgaacggtg gcgtgcgctc
     9901 cggcgacagc cagatgcgtt cgcccgtcga cgtcttgccc atcttctggc ccgtcgagtc
     9961 gagtaacagt ggcgcggtca ggccgaagat cttcgggcgg ccgagcttgt gctggagctc
    10021 ggtgccagcc gtgatgttgc cgtactgatc gttgccgccg acttgcagcc ggcagttgtg
    10081 gtgcttgttg agctcgacga agtcgaacgc ctgcagcagc atgtacgaga actcggtgta
    10141 gctgatgccg ttctcggact cgatgcggct cttcaccgag tccttcccca tcatgtaatt
    10201 gatggtcagg tacttgccga cgtcgcggag aaactcgagg aacgtgacgc cgcgagtcca
    10261 gtccgcgttg ttcgtcatca gcgcgccggt gcgcttgtca tcgaagtcga gcaggcgacc
    10321 gagctcgctg cggatgccgg cgaggttcgt cgcgagcgtc gcgtcgtcga gcaggttgcg
    10381 ctcgtcgctc ttgccggacg gatcgccgat catgccggtc gcgccgccga ccacgatgat
    10441 cgggcggtgc cccgcgagct gcagacgctt gagcaggatc gtcggcacga ggttgcccac
    10501 gtgcaggctc ggcgccgtcg ggtcatagcc ggcatacgcg gagaccgggg cctctttcgc
    10561 gagtagctcg gatagacctg cccgatctgt acagttcgcg tacaggccgc gggcttctag
    10621 ctcgtcgacg atcgacatgc ggcgcaccct agcacggagg atgcgttacg gcgcggcttc
    10681 ggcgagcagg ttcatgacga gcttcctgca gtccgcgaac actgctgggt gttcgtgctc
    10741 gtcggcgagc atgtcgacaa gccacgtggc gtcgaggagc cgcgacgtga gcagccagtt
    10801 gtcgggaagc cggccgccgg cgtcggtata gccgcgcgca aacgcctcgc agaacgcgcg
    10861 gtcgtagcgc ttcgaatagc gaaacaagct gccgatatcg acaagcggcg aaccggtcgt
    10921 cgtggtctcc caatcgatca ggccgttgat gcgccagcga tcgccggcct gataaacgag
    10981 cacgttgcga tggccgaggt caccatgcga gaggcaaacc gagccaaacg ccatcgcggg
    11041 ctctgccgct tccatcgcgc ggcgcaaccc atcagccatc ggcgcgccca tgcgtgcacg
    11101 cgcgcgaccg gtcgtcagct gtccgtacgc ttgctcgagg atcggcgtca gctcgaacgg
    11161 ctccgtcgcg tcggacttcg cgagccacgc gagcacgcga ccgagcggct cggcaagcgt
    11221 tgcgaacgcc gtgtgctcgc cttgcttgcg caggtcatgc agcgtgatgc cgtcgatcca
    11281 gcgatacacg atgacgaggc gtttctcgtc cgcgtgtacc acgtccggca ccgggagcgt
    11341 cccgtggagc agcgtgagcg tcgtgagctc ggtgtcgaac cagtccttgc cgccggcgta
    11401 gtgcttgacg ccgaacacgt cgccgctctc ggtcgtgacc ttgtggatcg tgttcgtcag
    11461 gccgccgttg acaggctcga tcgtcttgag cggaccgccg gccgcttctt cgaggtcttc
    11521 cttgctgact tgcagcagcg ggaagacccg tacatccgtt atgacggtat cgatgcgcaa
    11581 cgagccacca gcgtatcagc gcgcgtaatc ggacgcgcgg gtctcacgca cgacaacaac
    11641 cctgacctga ccgggatacg tggcttccgt ttcgatcgcc cttgcgatct ctttcgacat
    11701 gacgatcgcc tgcgcgtcgt ccacctgatc gttcatcacc atcacacgaa tttcacggcc
    11761 ggcctgaagc gcatacgcgc gctcgacacc gccgaaacgc ttacaaattg cttcgagatc
    11821 gtcgagtcgc ttgatgtacg aggcgagctg ctcccggcgc gcgccgggcc gtgctgccga
    11881 caacgcattc gctgcggcga cgatgtggtc gagtagcgcc tgtggtttgg ggccgccctc
    11941 gtcgccgtgg tgcgacgcga tcgcctggca cactttcggc gactcgccgt gcttgcggca
    12001 caacgccgca ccgacttcgg agtgcggacc ctcttgctcg tgatcgatcg ctttgccgac
    12061 gtcgtggagc agacccgcgc ggcgcgcgag cttgacgttc gcgccgagct ccgacgcgat
    12121 gaggccggcg agatagccga cctcgatcga gtggagcaga acgttctgcg cgtacgactg
    12181 gcggaacttc agccggccga gatgccgcac cagctcggga tgcacgcggt gcagaccgag
    12241 gtcgaacacc gcttgctcgc cggcttcctt gcagaccttg tcgacttcct tctcggcctt
    12301 gcgcacgcac tcttcgatgc gcgtcggatg aatgcggccg tcggcgacga gcgccgagat
    12361 cgcgatgcgc gcgacttcgc ggcgcaccgg gttaaagcac gagatcgtga tcgcttcggc
    12421 cgtgtcatcg atgatcatgt cgatgccggt cgccgcttcg agcgcgcgga tgttgcgacc
    12481 ttcgcggccg atcaggcgac ccttcagatc gtcggagggc aggggaatca cggcgaccgt
    12541 gcgctcgtga acgaactcgc tcgcgaaccg ctggatcgcc gttgcgacga tcgtcttggc
    12601 gcgtgcggtc gcttccttcg ccgcgtcgtc ttcgatgcgt ttgatctcga cggccgccga
    12661 cgcgagcgcc tgaccgcgca cttcttcttc gagcttcttg cgcgcagctt cttccgatag
    12721 tcccgcaatc tgctcgagct tcttgcgcgc gtccgcctgc gccgattcgg ctttctcgac
    12781 ggcagcgtcg gcctgcttga tcttgccctc gacgctcttc tctttcttgt cgagctcgcc
    12841 cgcgcgccgc tcggcgtctt tgcgctgctt gtcgatgtcg cgttcttttt gcgcgatcga
    12901 ttcttcgcgc ttctggagct cggctttgcg ctgcttcaga tcgtcttcga gctcggcctt
    12961 gcgcttgcgc gccgcctctt tgccctcgac ctctgctgct tgcttgatcg cttcgacctc
    13021 ggcgttcgcc gcggtgcgaa tcttctcggc ctcttcttcg gcgaccttgg ccgcgccgct
    13081 atcgccgcgc gcgcgaccga gccgcgtgcc gagaaacact ccggcgagga ggccgatcac
    13141 gacggccacg agaatgaaaa cgggttccat cagtccggtg ctcgatagat cgccgcctcg
    13201 gtgacgacgt agtgaagggc cgcgtcgtgc ggttcgcgcg gcacgtgctc gatgatctga
    13261 cactcgaacg cgagcccgat gcgcaacgcc gccggcgatg ccgccgcgag cgtcacgtcg
    13321 taatacccac gtccccaccc gatgcgacca ccggcgcgat cgaacgcgag accgggcacg
    13381 aacactgccg cgatctcgga gaggtc
//
LOCUS       DASUFX010000496        16059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1190067, whole genome shotgun sequence.
ACCESSION   DASUFX010000496 DASUFX010000000
VERSION     DASUFX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190067"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFV99_19895"
     CDS             <1..627
                     /locus_tag="VFV99_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5061644.1"
                     /translation="ERLVKITRRHDIPIIEDDVYGELVFDGARPRSLRSFAGSSDDNH
                     VVVVSSVSKTLAPGYRVGWIAGGRWHDQIVRLKFSQSLACPPLLGMAIAEFLSSGGYD
                     RHLRRLRTAVAGNVERYREAITEEFPEGTRVAAPRGGFVLWVELPPGVDALTLHEQAL
                     RRGIVVAPGPLFSARQRFANFIRISAGSPWNERVAAAMRTLGRLVGKS"
     gene            654..3485
                     /locus_tag="VFV99_19900"
     CDS             654..3485
                     /locus_tag="VFV99_19900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061645.1"
                     /translation="MTTTPGYETTIGVVDPTDPTAPEEPRSERRQELTRGTMVGRYQI
                     IKMIGRGGMGAVYAAHDPNLDRQVALKLLHSTSARNDDGAERMIREAQALARLDDPHV
                     VQVYDAGEYEDQIFITMQLVDGVDLGTAITHKKPGVPLLISWFCDAGRGLAAAHAAGL
                     VHRDFKPNNVLIDGRGRIAVTDFGLARTLEPSDERRHLTGVNAIMGTPAYMSPEQHGQ
                     QSAGPASDQFSFCVALWEALYDRHPFIAGDRSSIASMSPFAIGYQIFDGEIILPPKQR
                     HVPRRVHEALVRGLSRDPDKRWPSMTDLIAEIAPAQKRRIWPLVVGIGALAAAGGGVA
                     MWLVLANDPEQASCAQQSASRVTGAWSTTAAQALQAQFAKSGRSYAEKAAQQARTGLD
                     RYATRWQNLAADVCEAERAASGKVPELVVRRHACLDSRLDSFRGVTTMLTSEAKPEFV
                     DRAQAILDGLPDLADCIDESLPDVPPAAIATEVAKLDTELSAVEARAIAGDYATSKDA
                     ATKILARADQLKWPPLQVRAHFVIGRVQAALLLPARDELIAAGEIANANHLDREAARA
                     WTMAQQAAGYEQSADALATLAPIARSAAQRTNDKVLIATAEIKRARALVRMRKWQDGA
                     AACKAAYDNALTLDKKAAVDEARDCMLESLVPLGKGDDVEKLLAQILADKTTELGADH
                     PTISDYLKVRVGQRLRKGDLVGARKDAEQMLAIRRKTYPEKHYRIAEAIKGLSDVVAA
                     EGKADEADKLHREALAMLDETKSEHLVLLSALLVEIATREFISGKHKEGLERFEHAAK
                     LVRKRAGDNSLELAILLLNYGQFKASENVEAGLGLIGEAREILENNKDRRAPVAGIAA
                     AIIANNAKRYPDVVKFLDETLPLLGPNDDPEQIATAKSLLARALVETKGDKKRARQLA
                     TDAKTTFVKLGPSFDADVKVLDKVLAKLK"
     gene            complement(3468..5168)
                     /locus_tag="VFV99_19905"
     CDS             complement(3468..5168)
                     /locus_tag="VFV99_19905"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5061646.1"
                     /translation="MRSLLGRATRRPSIHLSDRDADGEQADENSHGLSSLYDREDGSH
                     VTRLAMPRMLDRVDRRSESQRLRHVRDELDLTQRQLAELFGVAASAIAQWESGQHTIP
                     GPVLRLLELYEREMGLGEQTVTKRLPFEATTRTARVAAASVLWLVFFGALQESEGNPL
                     LRQVREASLRRVTRMLGELKGLAMKLGQMISYADVIMTERERAVLSMLQTDTKPMSAA
                     AVCQVFVQELGKTPRQLFATWSADPIATASIGQVHEARLADGRSVAVKVQYPRIVQAL
                     EIDLGHAARFDRALALFFRGQQHPIILDELRARILEECDYTLEASNQGEAARAFAARS
                     DIRIAGVIPELSSRRILTSELLAGAPLLSFVKTASASARERAALALLDFYFQGLFLHR
                     FFHTDPAPTNFLFTDEHVCFLDFGRIKRFSQRFVDQLKQMLRAILSRDRDGVRAMLID
                     MGAVPDPHTYDFDYAYRALLHVHRPFLGEGAFAYTAEHLRKMWHVFIYANPNRSRINI
                     TPDMVFLNQFYFGVTAMLVRLGARVDYRARLLDLLYGPDEQRPAPFTDAEQRALLEFG
                     "
     gene            5167..6342
                     /locus_tag="VFV99_19910"
     CDS             5167..6342
                     /locus_tag="VFV99_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061647.1"
                     /translation="MIRRSALILGVCWLGQAPAPARADVYCGASNPNHDAPAGYLVLS
                     RSQDIVAPVVSALGEYRTHTMLSHGSWFTHAAMRTPSSGRNAPHLNSDQLRWGDPGAE
                     QISAASAYTFLYEPGNGGLEFISKQSGTSGGGSGSRAEELGISSYFWEDWPWRFVPAR
                     CEQRFMPSFSTYYDCTGTKRTAPYTYGGSSSVSVMTGGGYYRLRDDGGDIPYSLHQYV
                     DIGSLVTRGDRRCHFTNYGYLVCTDEGVVCSTLMAWGVKKALGRQIAVPTYTPQQTLD
                     AASALHHGVMSKCLNHVGIFESFASWLLGSGDEWQICDEMGWQVLNCMLDPNTCTLSY
                     QINSTVRASTVSPDSISGWRPGQDWGNPAINGPWTQAGWYEDMSFSGGGTTYTCWAN"
     gene            6344..7735
                     /locus_tag="VFV99_19915"
     CDS             6344..7735
                     /locus_tag="VFV99_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061648.1"
                     /translation="MSAQAPQKKSRRVLLLAGAALLWLVALSALVAVLRADEEGHRVA
                     HAAEPSGASRRITARPAMEAIHDKDDADATPIVIDEITVEKREICAGEENLVTVRAHA
                     AREEDAPELQIIIDGTPGSQVALRPTSYLGPDGTAPRVTAHLGDERRVSAEIPPFEVR
                     DCHPERILDVRHRAVPNAPGRVELWANVVQHGPGEQFKPVRYRWELSDGTREETTTPI
                     FEKSFRGRRQDALFTHVLVTCIAESADGQTLMGRTNIELFNQAYEILARKGTVLLEVD
                     HPQFPELDEKGIVNWPIRVWHHHNAPVTITAIRRIRVSRDGALGAGGGREQIDQVPVE
                     SVLGTNRIPSGWIDAKVTLDTHADPDVAYVLYEMTGRTDDGYPAGGGFSVMRPTELPT
                     REKHVQVADVQLMAKIIAAQKILGKQYVTDEDIWRLEREGKLDTATIDPADLPEHLPA
                     TEPGPKLGRSKGR"
     gene            7732..7869
                     /locus_tag="VFV99_19920"
     CDS             7732..7869
                     /locus_tag="VFV99_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061649.1"
                     /translation="MSITGIERELWLLALCYRARLPLPMLVVQRCLDQMTCSTRARAR
                     A"
     gene            complement(7871..8638)
                     /locus_tag="VFV99_19925"
     CDS             complement(7871..8638)
                     /locus_tag="VFV99_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5061650.1"
                     /translation="MNLSQLKIIVTGAAQGMGAHFARRLAEAGAQVAAGDVKEEGLAA
                     LAEATRGLPGKVHTRKLDVSSEADVTAFVDWAYGQMNGLNGLINNAGILRDGLLVKKD
                     KTTGEISKMSKENFDLVIGVNLTGATMMVRETVAKMVASEQKPGVIVNMSSIARHGNR
                     GQSNYVSAKAALAANTVTWAREFAQFGVRVCGVAPGMVETPMTQGMNQKARDAIVAQI
                     PVGRIGTPEDLWLAVKFCLECDYFNARTFDVDGGMGF"
     gene            complement(8715..10685)
                     /locus_tag="VFV99_19930"
     CDS             complement(8715..10685)
                     /locus_tag="VFV99_19930"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5061651.1"
                     /translation="MTSITRWLVLASLALAACARPPDSQECKTGITCPEGTKCAAAQP
                     ICITNDCGDGIQQSTEKCDDGNVIDGDGCAKDCLSKEVCGDNVINSAAGEICDDGNDD
                     GGDGCSADCKSVETCGNGIRDITEVCDDGNTVPGDGCSGNCKSTEVCGNGIVDINEKC
                     DDAGAPGNCNDDCQGGQGCGDGAIDKGADGLPIEECDDGNMFNDDDCVQCHIARCGDH
                     ELKTKGLNPEECDPAVNFGETFDCNMDCTNARCGDGKLNLTRGEQCDDTNTVDEDACK
                     NDCTINYCGDGVPGGPNEACDTAGPSMTCDFDCSMPGCGDGFVNLAFGEQCDSSGVNT
                     ATCDANCTIPICGDGTVNMAAGEDCDDMNNVNTDACRNDCKFNICGDGFTDSTDEMCD
                     DGNTMPETLCSYGTQTCQLCSANCQMVENLIGHFCGDTNMDSPFEVCDDGNSATESTC
                     PYNSPNGICTVCAGDCKSTPAKTGPYCGDGNVQMTFGEVCDDHNNMACGRCDATCHVQ
                     ASSAATGYIVAVSHTEFVDKNTITINDGFTHVETYEFDDDNMITSGNIKIAIGNMDTA
                     ADMAAKITSAINRGNGTLLIDAASTGALVTLTHQRKSSLGNVQIMAMVGNANFSYSGM
                     SGGTGGNCDPGIGCNSDDDCKSGNCMNNNQCQ"
     gene            complement(10698..11786)
                     /locus_tag="VFV99_19935"
     CDS             complement(10698..11786)
                     /locus_tag="VFV99_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061652.1"
                     /translation="MWGLLGVVLTFGSAHAQPKPPDPTPAASPSPAPAPNNGEKAGSD
                     ESLQKGGDNRPWAAGISQERQKAALLLFREGNSQLNDGLFARASETYRKALESWPHPA
                     IHYNLALAQMNLDQPIEAFENFQKAIAFGDAPLQTDKLEHAKEYMLLLEKQIAHIEVS
                     CDKAGAKVSVDGKEVFIAPGKFTQSVRIGRHTFVADKKGYQTRINAPFIGPGEHFRIE
                     LKLYTAEELTRFNRRWQATWMPYAVLGGGVAVGLVGVALELAADSSYNDYDKAVTACN
                     QNNMGCQTTTAITDLRDSGDQKKTLGYVAYGVAGAAVAAGALLAILNRPKAYQIRPEE
                     LQEEQEKKVSIKPILTPNLAGAMVQGKF"
     gene            complement(11822..15169)
                     /locus_tag="VFV99_19940"
     CDS             complement(11822..15169)
                     /locus_tag="VFV99_19940"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061653.1"
                     /translation="MSDDKPGKPADDPRLGPDAKTIINAPRPAARPSGEYAVSRPTDA
                     RMETGAKPTDRTIVSSPKPAAVNATMIGAPGPKKRPTMPGINVTPTAPSAAPPTVILS
                     SSEPTPIPAPPTMPQGTPVPQPVAAQGRATIASSGRARRPSEGPSGIAPRSAWDTPSG
                     IVPGSAPGSTPGTPPSTPGSGGSTGIHAASAQPHPGVRINQYEMIKMIGEGGMGTVYL
                     ARDLRLGRRVAIKFLQSNQQELTQRFLVEARATARCQHENIVVIYEVGEHVGAPYMVL
                     EYLNGKPLTSLIKGGGKLPYSRAVEIMVATLKALECAHGQGIVHRDLKPDNIFITDSG
                     SIKVLDFGIAKVLEATHVPQNSSGAIRMPSPLELATGSNTGLTRHGTIMGTLKYMSPE
                     QWGIGIEIDRLSDIWACGVLLHRLITGRHPLHPLDGNQLVVTAMLELPMPSMAEAAPP
                     DCPRELITIVDRCLLKMKEQRPQSAAELLRALEPFLPGRRSTELQIDESPYAGLSSFQ
                     ENDAGKFFGRNREIAAMVTRIRDRPLMAVVGSSGVGKSSFVRAGLVPALKRSGEQWET
                     LVTRPGRKPMDALAAIIAPLIGTASNVADDMAAQAKLAEKLRNEPGHLGHVLRHRARR
                     DSRRIMVFVDQFEELYTQVPDAAERAAFTACLSAVADDATSPLRVVLSIRSDFLDRVA
                     EDQQFTAELTQGLFFLNQPNRDGLKEAIVHPAELAGYSFEYPTTVEDMLDHLETTPGA
                     LPLLQFAASKLWETRDAGRKMLTHTSYMAMGGVAGALASHADSVVQDLGQHRQQLVRA
                     VLLRLVTPERTRAIVPLEELRDLSREVGEVQRLIDQMVDARLLVVQTLEGGKGSTVEI
                     VHESLIQNWPTLRRWLDETQDDAAIIDQLRTASRQWNAKGRSADLLWRGDTAEEAKKF
                     RRRYKGPLSDIEQAFLDEIILYEQAVSRRKRVAVIGAFIGMAILVVAALVALVVIQKS
                     RNDAKQNQLVAEQKTAEAVKLKIEADENLAAMRQKEKERQAAEAAKQVVDVKLDVAEE
                     DLKKKNAELELALKDAQENEAQAKHAEERAKAAQKSAEKAQGEAVAAKDEVLFEKAKV
                     EALWRQEQERVRRLQSQIGSPIVDDLK"
     gene            complement(15369..>16059)
                     /locus_tag="VFV99_19945"
     CDS             complement(15369..>16059)
                     /locus_tag="VFV99_19945"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5061654.1"
                     /translation="IADVLATQIGDLCSVLMLSDDGETLEIVAIRGRDTDVVAIATNA
                     FLGKRLAVMPIQRRAMNLGTTFTARHLTAGASRFLTTPERAAFAERIGVHSVTTIGLR
                     TRESAVGVLNLLRHGPDHQPYDDADRDLLQRLALPVARAVGNARLLRQQLDALRGAHA
                     VAVRALGELDALAVMASDALSSPLPAVRGYAEALHDQIGELVRNAGAEHLPRMRVDAT
                     ELRVTIDDLLR"
BASE COUNT         2641 a         5255 c         5283 g         2880 t
ORIGIN      
        1 gagcgtctcg tcaagatcac gcgtcgccac gacatcccga tcatcgagga cgacgtctac
       61 ggcgagctcg tgttcgacgg cgcgcggccg cgttcgctgc gctcgttcgc tggctcgagc
      121 gatgacaacc acgtggtggt cgtcagctcg gtgagcaaga cgctcgcgcc tggctatcgg
      181 gtgggctgga tcgccggcgg tcgctggcac gatcagatcg tacggctgaa gttctcgcag
      241 tcgcttgcgt gcccgccact gctcggcatg gcgatcgccg agttcttgtc gtcgggcggc
      301 tacgatcggc atctgcgccg tctgcgcacc gcagtcgccg gtaacgtcga gcggtaccgc
      361 gaggcgatca ccgaggagtt tcccgagggg acccgcgtgg cggcgccgcg cggcggattc
      421 gtgctgtggg tcgagctgcc acctggcgtc gacgcactca cgctccacga gcaggctttg
      481 cgccgcggca ttgttgttgc gccgggtccg ctgttttctg cgcggcagag attcgcgaat
      541 ttcatacgca tctctgccgg ctcgccatgg aacgagcgcg tggcagcagc catgcgaaca
      601 ctcgggcgcc tggtcgggaa atcctagccg gggttcaggc gtatatttcg gcggtgacga
      661 cgacgccggg gtacgagacg acgatcggcg tcgtcgatcc gactgatcca accgcacccg
      721 aggagccgcg cagcgaacga cgccaggagc tcacgcgcgg cacgatggtc ggtcgctacc
      781 agatcatcaa gatgatcggt cgcggcggca tgggggcggt ctacgccgcc cacgatccca
      841 atctcgatcg gcaggtcgcg ctcaagctgc tgcactcgac gtccgcgcgt aacgacgacg
      901 gcgcggagcg catgatccgc gaggcgcagg cgctcgcgcg actcgacgat ccgcatgtcg
      961 tgcaggtcta cgacgcaggc gagtacgagg accagatctt catcacgatg cagctcgtcg
     1021 atggcgtcga cctcggcact gcgatcacgc acaagaagcc cggcgtgccg ctgctcatct
     1081 cgtggttctg cgacgccggc cgtggcctcg ctgccgcaca cgctgccggt ctcgttcacc
     1141 gcgacttcaa gccgaacaac gtgctcatcg atgggcgcgg ccgcatcgcg gtcaccgact
     1201 tcgggctcgc gcgcacgctc gagccgagcg acgagcgccg tcatctcact ggcgtcaacg
     1261 cgatcatggg cacgccggcg tatatgtcgc cggagcagca cggtcagcag agcgccggac
     1321 ccgcgagcga tcaattctcg ttctgcgtcg cgctgtggga agcgctctac gatcgccatc
     1381 cgttcatcgc aggcgatcgc tcgagcatcg cgtcgatgtc gccgttcgcg atcggctatc
     1441 agatcttcga cggcgaaatc atcttgccgc cgaaacaacg ccacgtgccc cgccgcgtgc
     1501 acgaggcgct cgtccgcggg ttgtcgcgcg atccggacaa gcgttggccg tcgatgacgg
     1561 acctcatcgc cgagatcgca ccggcacaga agcgtcggat ctggccgctc gtcgtcggca
     1621 tcggcgcgct cgccgcggcc ggcggcggtg tcgcgatgtg gctcgtgctc gcgaacgatc
     1681 ccgagcaggc gagttgcgcg cagcaatccg ccagtcgcgt gaccggagcg tggtcgacca
     1741 ccgcggcgca ggcactgcag gcgcagttcg cgaagagtgg tcgctcgtac gcggagaagg
     1801 cggcacagca ggcgcgcacc gggctcgatc gctacgcgac gcgctggcag aacctggcgg
     1861 ccgatgtctg cgaagcggag cgtgcggcgt cgggcaaggt gcccgagctg gtcgtgcgcc
     1921 gtcacgcgtg tctcgacagc cggctcgatt cgttccgcgg cgtcacgacg atgctgacga
     1981 gtgaagcgaa accggagttc gtcgatcgcg cgcaggcgat cctcgacgga ttgcccgatc
     2041 ttgcggactg catcgacgag tcattgcccg acgtgccgcc cgccgcgata gccaccgagg
     2101 tcgcgaagct cgacaccgag ctgtcggccg tcgaggcacg cgcgatcgcc ggtgactacg
     2161 cgacgagcaa ggacgcggcg acaaagattc tggcgcgtgc ggatcaactg aaatggccgc
     2221 cgctgcaggt acgtgcgcac ttcgtgatcg gccgcgtgca ggcggcgttg ctcctgccgg
     2281 cgcgggacga gctgatcgcc gccggcgaga tcgcgaatgc gaaccacctc gatcgcgagg
     2341 cggcgcgcgc gtggacgatg gctcagcagg cggcaggcta cgagcaatcc gccgacgcgc
     2401 tggcgacgct cgcgccgatc gcgcggtcgg ctgcgcagcg cacgaacgac aaggttctga
     2461 tcgcgacggc cgagatcaag cgtgcgcgcg cgctcgtccg catgcgcaag tggcaagacg
     2521 gtgccgccgc gtgcaaggcc gcgtacgaca acgccctgac gctcgacaag aaggctgccg
     2581 tcgacgaagc gcgcgactgc atgctcgaat cgctggtgcc gctcggcaag ggcgacgacg
     2641 tcgagaagct gctcgcgcag atcctcgccg acaagaccac cgagctcggc gccgatcacc
     2701 cgacgatctc cgactacctc aaagtgcgcg tcggtcaacg cctgcgcaaa ggcgacctcg
     2761 tcggcgcgcg caaggacgcc gagcagatgc tcgcgatccg gcgcaagacg tatcccgaga
     2821 agcactaccg gatcgccgag gcgatcaagg gcctctccga cgtcgtggct gccgagggca
     2881 aggccgacga agcggacaag ctccaccgcg aagcgctcgc gatgctcgac gagacgaagt
     2941 cggagcactt ggttctcctc agcgcgctgc tcgtcgagat cgcgacccgc gagttcatct
     3001 ccggcaagca caaagaaggt ctcgagcgct tcgagcacgc ggcgaagctc gtgcgcaaac
     3061 gagccggcga caactcgctc gagctcgcga tcctgctgct caattacggc cagttcaaag
     3121 cgagcgagaa cgtcgaagcc ggtctcggcc tgatcggcga ggcgcgcgag atcctcgaga
     3181 acaacaagga tcggcgcgcg ccggtcgccg gcatcgcggc cgcgatcatc gcgaacaatg
     3241 caaagcgcta tcccgacgtc gtcaagttcc tcgacgagac gctacctctg ctcggcccca
     3301 acgacgatcc cgagcagatc gcgaccgcga agtcactgct cgcacgcgcc ctcgtcgaga
     3361 ccaagggcga caagaagcga gcgcgtcagc tcgcgaccga cgcgaagacg acgttcgtca
     3421 agctcggtcc gtcgttcgac gccgacgtca aagtactcga caaggtgcta gccaaactca
     3481 agtagcgcgc gctgctccgc atccgtgaat ggagcagggc gctgctcgtc tggtccgtac
     3541 agaagatcga gcaaccgcgc gcggtaatcg acgcgggcgc ccagccgaac cagcatcgcg
     3601 gtcacgccaa agtagaactg gttcaagaac accatgtcgg gcgtgatgtt gatgcgcgag
     3661 cggttcgggt tcgcgtagat gaacacgtgc cacatcttgc gcaaatgctc ggcggtgtac
     3721 gcaaacgcgc cctcgccgag gaacgggcga tggacgtgga gcagcgctcg atacgcgtaa
     3781 tcgaagtcat acgtgtgcgg atcgggcacg gcacccatat cgatcagcat cgcgcgcacg
     3841 ccgtcgcggt cgcgtgacag gatcgcgcgc agcatctgct tcagctgatc gacgaagcgc
     3901 tgagagaagc gcttgatacg gccgaagtcg aggaagcaga cgtgctcgtc ggtgaacaga
     3961 aagttcgtcg gtgcagggtc cgtgtgaaag aaccggtgca ggaacagccc ttgaaaatag
     4021 aagtcgagca gcgcgagcgc cgcgcgttca cgagcactcg cgctcgcggt tttgacgaac
     4081 gacagcagcg gtgcgccggc gagcagctcc gaggtcagaa tgcggcggct ggagagctcc
     4141 gggatcacgc ctgcgatccg gatgtcggaa cgcgcagcga atgcacgtgc ggcctcgcct
     4201 tggtttgacg cctcgagcgt gtagtcgcat tcctcgagaa tgcgcgcgcg aagctcgtcg
     4261 aggatgatcg gatgttgctg cccgcggaag aacagcgcga gtgcgcgatc gaagcgcgcc
     4321 gcgtggccga gatcgatctc gagcgcttgg acgatgcgcg ggtattgcac tttcaccgcg
     4381 acggagcggc catcggccag ccgggcttcg tgcacctgac cgatgctcgc ggtcgcgatc
     4441 ggatctgcgg accacgtcgc gaacagttgg cgcggtgtct tgccgagctc ctgcacgaac
     4501 acctggcaca ctgctgccgc tgacatcggt ttggtgtctg tctgcagcat cgagagcacg
     4561 gcccgttcgc gctcggtcat gatgacatcg gcgtaactga tcatctggcc gagcttcatc
     4621 gcgagaccct tgagctcgcc gagcatccga gtgacacggc gaagcgacgc ttcacgcact
     4681 tggcggagca gtgggttgcc ctcgctctcc tgtaacgcgc caaagaacac gagccacaac
     4741 accgacgccg cggcgacgcg cgcggtacgt gtcgttgcct cgaacgggag ccgcttcgtc
     4801 accgtttgct cgccgagtcc catctcgcgc tcgtagagct cgagcaggcg cagcactgga
     4861 cctgggatgg tgtgctgacc gctctcccat tgtgcgatcg cgctcgccgc gacgccgaac
     4921 agctcggcga gctgccgctg cgtcagatcg agctcgtccc gaacgtgtcg aagtcgttgc
     4981 gactccgacc gccggtcgac gcgatcgagc atccgcggca ttgccaacct cgtaacatgg
     5041 ctgccgtctt cccgatcgta caggctgctc aggccgtgac tattttcgtc cgcctgttca
     5101 ccgtcggcat ctcggtcact tagatggatg ctggggcgtc gtgtggctcg ccccaggagg
     5161 gaccgcatga ttcgaaggtc tgcactgatc ctcggggtgt gctggctcgg ccaggctccc
     5221 gcccccgcac gtgccgacgt ctactgcggc gcgagtaatc cgaaccacga cgcgccggcc
     5281 ggctacctcg tgctcagccg gtcgcaagac atcgtcgcgc cggtcgtgag tgcactcggc
     5341 gagtaccgca ctcacaccat gctctcgcat ggcagctggt tcacgcacgc agcgatgcgg
     5401 acgccgagct cgggacgcaa tgcaccgcac ctcaactcgg atcagctccg ctggggcgat
     5461 ccgggtgccg aacagatcag cgcagcctcg gcgtacacgt ttctgtacga gccaggcaac
     5521 ggtggcctcg agttcatctc gaagcagtca ggcacgtcgg gcggcggatc gggatcacgc
     5581 gccgaagagc tcggcatctc gtcgtacttc tgggaggact ggccgtggcg ctttgtgccg
     5641 gcccgctgcg agcagcggtt catgccctcg ttctcgacgt actacgactg cacgggcacc
     5701 aagcgcacgg ctccgtatac ctacggcggc agctcgtcgg tgtcggtgat gactggcggc
     5761 ggttactacc ggctgcgcga cgatggcggc gacattccgt acagcctgca tcagtacgtc
     5821 gatatcggca gcctcgtcac gcgcggtgat cgccgctgcc acttcacgaa ctacggctac
     5881 ctcgtgtgca ccgacgaggg tgtcgtgtgc tcgacactga tggcgtgggg cgtgaagaaa
     5941 gcgctcggcc gccagatcgc ggtgccgacg tacacgccgc agcagacgct agatgccgcg
     6001 tccgcacttc atcacggtgt gatgagcaag tgcctcaacc acgtcggcat cttcgagagc
     6061 tttgcgagct ggctcctcgg cagtggtgac gaatggcaga tctgcgacga gatgggctgg
     6121 caggtgctca actgcatgct cgatccgaat acctgcacgc tgagctatca aatcaactcg
     6181 acggtgcgcg cgtcgacagt gagccccgac tccatctcgg gctggcggcc gggacaggac
     6241 tggggcaacc cggcgatcaa cggaccgtgg acgcaggcag gttggtacga ggacatgagc
     6301 ttcagcggcg gcggcacgac ttacacctgc tgggccaact gacatgagtg cgcaagctcc
     6361 gcagaagaag tcgcgacgcg tgttgttgct cgccggtgcc gcgctgctat ggctcgtcgc
     6421 gctgagcgcg ctcgttgccg tgcttcgcgc cgacgaggag gggcaccgcg tggcgcatgc
     6481 agccgagccg tccggtgcgt ctcgccggat cacggcgcgt cctgcgatgg aagcaatcca
     6541 cgacaaggac gacgcagacg ccacgccgat cgtgatcgac gagatcaccg tcgagaagcg
     6601 agagatctgc gcgggcgaag agaacctcgt gaccgtgcgc gcccacgccg cgcgggaaga
     6661 ggatgcaccc gagctgcaga tcatcatcga tggcacgccc ggttcgcagg tcgcgctgcg
     6721 cccgacgagc tatctcggcc cggatggcac ggcaccgcgc gtgaccgcgc atctcggcga
     6781 cgagcgccgc gtcagcgccg agattccgcc gttcgaggtt cgcgactgcc accccgagcg
     6841 catcctcgac gtccgccacc gtgcagtgcc caacgcgccg gggcgcgtcg agctatgggc
     6901 caacgtcgtc cagcatggcc ccggcgaaca gttcaagccg gtgcgctatc gctgggagct
     6961 cagcgacggc acgcgcgagg aaacgacgac gccgatcttc gagaagagct ttcgcggtcg
     7021 gcggcaggac gcgctcttca cgcacgtgct cgtgacgtgc atcgcggaga gcgcggatgg
     7081 ccagacgttg atgggacgta ccaacatcga gctgttcaat caggcgtacg agatccttgc
     7141 ccgcaagggc accgtgctcc tcgaggtcga tcacccgcag tttcccgagc tcgacgagaa
     7201 gggcatcgtg aactggccga tccgcgtatg gcatcaccac aacgcgccgg tcacgatcac
     7261 cgcaatccgc cggattcggg tgtcacgcga cggcgcactc ggggcaggtg gtggccgcga
     7321 gcagatcgat caggttccgg tcgaatcggt gctgggcacg aaccggatcc cgtcgggctg
     7381 gatcgatgcg aaggtcacgc tcgacacgca tgccgatcct gatgtcgcgt acgtgttgta
     7441 cgagatgacc gggcggaccg acgacggcta cccggctggc ggcggcttct cggtgatgcg
     7501 cccgaccgag ctaccgacac gtgagaagca cgtgcaggtc gccgacgttc agctgatggc
     7561 gaagatcatc gccgcgcaga agatcctcgg caagcagtac gtcaccgacg aggacatctg
     7621 gcggctcgag cgcgagggca agctcgacac cgcgacgatc gatcctgcgg acctgcccga
     7681 gcatctgccc gcgacggagc cgggaccgaa gctcggccga agcaaagggc gatgagcatc
     7741 accgggatcg agcgagagct gtggctgctc gcgctgtgct atcgcgcgag gctaccgctg
     7801 cccatgctgg tcgtgcagcg gtgcctcgat cagatgacgt gcagcacgcg tgcaagagca
     7861 cgcgcgtagg ctagaagccc atgccgccgt cgacgtcgaa cgtgcgcgcg ttgaagtagt
     7921 cgcactcgag gcagaacttc acggcgagcc agaggtcctc gggcgtgccg atgcggccga
     7981 ccggaatctg cgccacgatc gcatcgcgcg ccttctggtt catgccctgc gtcatcggcg
     8041 tctcgaccat gcccggcgcg acgccgcaca cgcgaacgcc gaactgcgca aactcacgcg
     8101 cccacgtcac cgtgttcgcc gcgagcgcag ccttcgccga tacgtagttc gactggccgc
     8161 ggttaccgtg gcgcgcgatc gacgacatgt tgacgatcac gcccggcttc tgctcgctcg
     8221 cgaccatctt cgcgacggtc tcgcggacca tcatcgtcgc gcccgtgagg ttcacgccga
     8281 tcacgaggtc gaagttctct ttgctcatct tcgagatctc gcccgtcgtc ttgtccttct
     8341 tgacgagcag tccgtcgcgc aggatgccgg cattgttgat gaggccgttg aggccgttca
     8401 tctggccgta cgcccagtcg acaaacgcgg tgacatccgc ttcgctcgag acgtcgagct
     8461 tgcgcgtgtg caccttaccc ggcagtccgc gcgtcgcttc agcgagcgcc gcgaggccct
     8521 cttccttgac gtcgcccgcg gcgacctgag cgccagcctc ggcgaggcgg cgagcgaagt
     8581 gcgcgcccat gccctgggct gcaccggtga cgatgatctt gagctgcgac aggttcatgg
     8641 cgccttctta tcattgctgc ggtgcaacaa ccagtgctgc gtcgcagcaa tcgtgccgag
     8701 cgccgttcag tgggtcactg gcactggttg ttgttcatgc aattgcccga cttgcagtcg
     8761 tcgtcgctgt tgcagccgat acccgggtcg cagttgccgc cggtgccgcc ggacatgccc
     8821 gagtaggaga agttggcgtt gccgaccatc gccatgatct ggacgttacc gaggctgctc
     8881 ttgcgctgat gagtgagcgt gacgagcgcg ccggtgctgg ccgcatcgat gagcagcgtg
     8941 ccgttgccac ggttgatcgc gctcgtgatc ttggcggcca tgtcggcggc ggtgtccatg
     9001 ttgccgatcg cgatcttgat gttgccgctc gtgatcatgt tgtcgtcgtc gaattcgtac
     9061 gtctcgacat gcgtgaagcc gtcgttgatg gtgatcgtgt tcttgtcgac gaactcggtg
     9121 tggctcaccg caacgatgta gccggtcgca gccgacgacg cttgcacgtg gcaggtcgca
     9181 tcgcagcggc cacacgccat gttgttgtgg tcgtcgcaca cctcgccgaa cgtcatctgg
     9241 acgttgccgt caccgcagta cgggccggtc ttcgccggcg tcgacttgca atcgcccgcg
     9301 cacaccgtgc agatcccgtt cggcgagttg tacgggcacg ttgactcggt cgcgctgtta
     9361 ccgtcgtcgc agacctcgaa cggcgaatcc atgttcgtgt cgccgcagaa gtgaccgatg
     9421 aggttctcca ccatctggca gttcgcgctg cacagctggc aggtctgcgt gccgtacgaa
     9481 cataacgtct ccggcatcgt gttgccgtcg tcgcacatct cgtcggtgct atcggtgaag
     9541 ccgtcgccgc agatgttgaa cttgcagtcg ttgcggcacg cgtcggtatt gacgttgttc
     9601 atgtcgtcgc agtcttcacc ggctgccatg ttcacggtgc cgtcgccgca gatcggaatc
     9661 gtgcagttcg catcgcaggt cgccgtgttg acgccggagc tatcgcattg ctcgccgaac
     9721 gcgaggttga cgaagccgtc gccacacccc ggcatcgagc agtcgaagtc gcacgtcatc
     9781 gacgggccgg cggtatcgca cgcttcgttg ggaccgccgg ggacgccgtc gccacagtag
     9841 ttgatcgtgc agtcgttctt gcacgcgtct tcgtcgacgg tgttcgtgtc gtcgcactgc
     9901 tcgccacgcg tcaggttgag cttgccgtcg ccgcagcgcg cgttcgtgca gtccatgttg
     9961 cagtcgaacg tctcgccaaa gttgacggcc ggatcgcact cttccgggtt aaggcccttg
    10021 gtcttcagct cgtgatcacc gcagcgcgcg atgtggcact ggacgcagtc gtcgtcattg
    10081 aacatgttgc cgtcgtcgca ctcttcgatc ggtaggccgt cggcgccctt gtcgatcgcg
    10141 ccgtcgccgc agccctgacc gccctggcag tcgtcattgc agttaccggg ggcgccagcg
    10201 tcgtcgcact tctcgttgat gtcgacgatg ccgttgccgc agacctcggt cgacttgcag
    10261 ttgccggagc aaccgtcgcc gggcaccgtg ttgccgtcgt cgcaaacttc ggtgatgtcg
    10321 cggatgccgt tgccacacgt ctcgactgac ttgcagtccg ccgagcagcc atcgccgccg
    10381 tcgtcgttac cgtcgtcgca gatttcgccg gcggccgagt tgatcacgtt gtcgccgcag
    10441 acttctttcg agaggcagtc cttggcgcag ccatcgccgt cgatgacgtt gccgtcgtcg
    10501 cacttctcgg tcgactgctg gatgccatca ccgcagtcgt tcgtgatgca gatcggctgc
    10561 gcggcggcgc acttcgtacc ttcggggcac gtgatgccgg tcttgcactc ctgcgagtca
    10621 ggtgggcgcg cgcaagccgc gagcgcgagt gacgcgagaa caagccaacg tgtaatcgaa
    10681 gtcatcgata atctccgtta gaactttccc tgcaccatcg cgccagccag gtttggcgtg
    10741 aggatcggtt tgatcgagac cttcttctct tgctcctctt ggagctcctc gggccggatc
    10801 tggtaggcct tcgggcgatt gaggattgcg agcagcgcgc cggcagcgac ggccgcaccg
    10861 gcgacgccgt acgcgacgta gccgagcgtc ttcttctggt cgccggagtc gcgcagatcg
    10921 gtgatcgcgg tcgtcgtctg gcaacccatg ttgttctggt tgcaggccgt gacggccttg
    10981 tcgtagtcgt tgtacgacga gtcggccgcg agctcgagcg cgacgcccac gagaccgacg
    11041 gcgacaccgc cgccgagcac cgcgtacggc atccacgttg cctgccagcg ccggttgaag
    11101 cgagtcagct cttcggccgt gtacagcttg agctcgatcc ggaagtgctc gcccgggccg
    11161 atgaacggcg cattgatccg cgtctggtag cccttcttgt cggcgacgaa cgtgtggcgg
    11221 ccgatgcgca cgctctgcgt gaacttgcct ggtgcgatga agacctcctt gccgtcgacg
    11281 gagaccttgg cgcccgcttt gtcgcagctc acctcgatgt gcgcgatctg cttttcgagc
    11341 aggagcatgt actcctttgc gtgctcgagc ttgtccgtct gcagcggcgc atcaccgaac
    11401 gcgatcgcct tctggaaatt ctcgaatgcc tcgatcggct ggtcgaggtt catctgcgcg
    11461 agcgcgaggt tgtagtggat cgcggggtgc ggccagctct cgagcgcctt gcgataggtc
    11521 tcgctcgcgc gcgcgaacaa gccgtcgttg agctgcgagt tgccttcgcg gaacaacagc
    11581 agcgccgcct tctgacgctc ctgcgagata ccggctgccc acggacggtt gtcgccgccc
    11641 ttctgcaatg actcgtcgct gcccgccttc tcgccattgt tcggtgccgg cgccggcgat
    11701 ggcgacgcag ccggtgtcgg atccggcggc ttcggttgcg catgcgcgct gccgaaagtc
    11761 agcacgacgc cgagcaatcc ccacattgcc ttggtcataa gttctccagc gggctcaact
    11821 atcatttgag atcgtcgacg atcgggctac cgatctgcga ctgcagacgt cgcacgcgtt
    11881 cctgctcttg gcgccacagt gcctcgacct tcgccttctc gaacaatact tcgtccttcg
    11941 ccgcgacggc ttcaccctgt gccttctcgg cggacttctg cgcagccttc gcacgttcct
    12001 ccgcgtgctt cgcctgcgcc tcgttctcct gggcgtcctt gagcgcgagc tcgagctcgg
    12061 cgttcttctt cttcagatct tcctcggcta cgtcgagctt cacgtcgacg acttgcttcg
    12121 ccgcctccgc cgcttgccgc tctttctctt tctggcgcat cgccgcgagg ttctcgtcgg
    12181 cttcgatctt cagcttcacc gcttccgctg tcttctgctc ggcgaccagc tggttctgct
    12241 tcgcatcgtt gcgcgatttc tggatcacga cgagcgcgac gagtgccgcg acgacgagga
    12301 tcgccatgcc gatgaacgcg ccgatcaccg cgacgcgctt gcggcgtgac accgcctgct
    12361 cgtagaggat gatctcgtcg aggaacgcct gctcgatatc cgagagcgga cctttgtagc
    12421 ggcgacggaa cttcttcgct tcctcggcgg tatcgccgcg ccacaacaag tctgccgagc
    12481 gccccttcgc gttccattgg cgcgacgcgg tccgcagctg atcgatgatc gcagcgtcgt
    12541 cctgcgtctc gtcgagccag cgacgtaacg tcggccagtt ctggatcagc gactcgtgca
    12601 cgatttcaac ggtcgagcct ttgccaccct cgagcgtctg tacgacgagc aggcgcgcat
    12661 cgaccatctg atcgatcagg cgctgcacct cgccgacctc gcgcgacagg tcgcgcagct
    12721 cctcgagcgg aacgatcgca cgcgtccgtt cgggcgtcac gaggcgcagc agcaccgctc
    12781 ggaccagctg ttgcctgtgc tgaccgaggt cctggaccac gctgtcggcg tggctcgcga
    12841 gtgcaccggc gacaccgccc atcgccatgt agctcgtgtg cgtgagcatc ttgcggccag
    12901 cgtcgcgggt ctcccatagc ttgctcgccg cgaactgcag cagcggtagc gcgccgggcg
    12961 tggtctcgag gtgatcgagc atgtcctcga cggtcgtcgg gtactcgaac gagtagccag
    13021 cgagctctgc cggatgcacg atcgcctcct tgaggccgtc gcggttgggc tgattcaaga
    13081 agaacaagcc ctgcgtgagc tcggcggtga actgctggtc ctcggcgacg cgatcgagaa
    13141 agtcggagcg gatcgacagc acgacgcgca gcgggctggt cgcgtcgtcc gcgacggctg
    13201 acagacacgc ggtgaacgcc gcgcgctcgg ccgcatccgg cacctgcgtg tagagctcct
    13261 cgaactggtc gacgaacacc atgatgcggc ggctatcgcg acgcgcgcgg tggcgcagca
    13321 cgtggccgag gtgaccgggc tcgttgcgca gcttctcggc gagcttcgcc tgcgccgcca
    13381 tgtcgtcggc gacgttcgat gccgtgccga tgagcggcgc gatgatcgca gcgagcgcat
    13441 ccatcggctt gcggccgggg cgcgtgacga gcgtctccca ctgctcgcca gaacgcttca
    13501 acgcggggac gagaccggcg cgcacgaacg acgacttgcc gacgccggag ctaccgacga
    13561 ctgccatcag cggccgatcg cggatcctcg tcaccatcgc cgcgatctcg cggttacggc
    13621 cgaagaactt gccggcgtcg ttctcctgga acgacgacag accggcgtac gggctctcgt
    13681 cgatctgcag ttccgtcgag cggcgacccg gcaagaacgg ctcgagcgcg cgtagcaact
    13741 cggccgcgct ttgcgggcgc tgctccttca tcttgagcag gcagcgatcg acgatggtga
    13801 tcagctcgcg tgggcagtcg ggcggcgccg cttcggccat cgacggcatc ggcagctcga
    13861 gcatcgcggt gacgacgagc tggttaccgt cgagcgggtg cagcgggtgg cgaccggtga
    13921 tcaaacgatg cagcagcacg ccgcacgccc agatatcgct caggcgatcg atctcgatgc
    13981 caatgcccca ctgttcaggg gacatgtact tgagcgtgcc catgatcgtg ccgtgccgcg
    14041 tgaggccggt gttcgatccg gtcgccagct cgagtgggct cggcatgcga atcgcgcccg
    14101 acgaattctg tggcacgtgc gtcgcctcga gcaccttcgc gatgccgaag tcgagcacct
    14161 tgatgctgcc gctgtcggtg atgaagatgt tgtcgggctt gaggtcgcgg tgaacgatgc
    14221 cctggccgtg cgcgcactcg agtgccttga gcgtcgcgac catgatctcg acggcgcgcg
    14281 agtagggcag cttgccgccg cccttgatca ggctcgtcag cggcttgccg ttcaggtact
    14341 cgagcaccat gtacggcgcg ccgacgtgct cgccgacttc gtagatgacg acgatgtttt
    14401 cgtgctggca gcgtgcggtt gcacgcgctt cgacgaggaa gcgctgggtc agctcctgct
    14461 gattggactg caagaacttg atcgcgacgc gacgaccgag tcgcaagtcg cgggcgaggt
    14521 agacggtgcc cataccgcct tcgccgatca tcttgatcat ctcgtactgg ttgatgcgaa
    14581 caccgggatg cggctgcgcc gatgcagcgt ggatcccggt ggatcctccc gagccgggtg
    14641 tgctcggtgg cgtgccgggc gtgctgccgg gcgcgctgcc aggaacgatg ccgctcggcg
    14701 tatcccacgc agagcgcggc gcgatcccgg atgggccttc cgagggacgg cgcgcgcggc
    14761 ccgagctcgc gatcgtggcg cgaccctgcg cggcgacggg ctgcggaacc ggcgtaccct
    14821 gcggcatcgt cggcggtgct ggaatcggtg tcggctccga cgacgagagg atgaccgtcg
    14881 gcggtgcggc ggacggcgcg gttggtgtga cgttgatgcc gggcatcgtc ggacgcttct
    14941 tcggcccggg cgcaccgatc atggtcgcgt tgacggctgc aggcttgggt gagctgacga
    15001 tcgtgcgatc cgttggcttc gcgccggttt ccatccgcgc atcggtcggt cgggacaccg
    15061 cgtactcgcc gctcgggcgt gccgcaggtc gaggcgcatt gataatcgtt ttggcgtcgg
    15121 ggcctagccg agggtcgtcg gccggtttcc caggcttgtc gtcactcatt cgtagtgcct
    15181 ccgcgaggta cccctggcag acgtagtccg cccccctact gaacgccggc catgattgta
    15241 caagccgcca cgcaaagttc gcgtgagcca catggccaat tgcccccgcc tacatctgct
    15301 cgctacccca gtgttcaccc acgaatactg tgacgattcg agcagtcgga gaacttcgcg
    15361 ggcttcggct agcgcaacag atcgtcgatc gtgacacgca gctcggtcgc gtcgactcgc
    15421 atgcgcggca ggtgctcggc gccagcgttg cgaacaagct cgccgatctg gtcgtgcagc
    15481 gcttcggcat atccacgcac agccggcaac ggagagctga gcgcatcgct cgccatgacg
    15541 gccagtgcgt cgagctcgcc gagtgcgcgc accgcgactg cgtgtgcgcc gcggagcgcg
    15601 tcgagctgtt gtcgcaacag cctcgcgttt ccgaccgcgc gtgcgaccgg cagcgccaac
    15661 ctctgcaaga ggtcgcgatc ggcgtcgtca tacggctggt ggtccgggcc gtgccgcaag
    15721 agattgagca cgccgaccgc ggactcgcgc gtgcgcaatc cgatcgtcgt cacgctgtgc
    15781 acgccgatgc gctcggcgaa tgcagcgcgc tctggcgtcg tgaggaacct gctagcgccg
    15841 gcggtgaggt gacgtgcggt gaacgtcgtg ccgaggttca tcgcgcggcg ctggatcggc
    15901 atgacagcga gccgcttgcc gaggaacgcg ttcgtcgcga tcgcaacgac atcagtatca
    15961 cggccacgaa tcgcgacgat ctcgagcgtc tcgccatcgt ccgacagcat caacaccgag
    16021 cagaggtcgc cgatctgcgt cgcgagcacg tctgcaatc
//
LOCUS       DASUFX010000497         8605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_774021,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000497 DASUFX010000000
VERSION     DASUFX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774021"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFV99_19950"
     CDS             <1..988
                     /locus_tag="VFV99_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061655.1"
                     /translation="TRGKVDKKIGAATLEMLRLAQPRVDGPAAAEIAALIAELEQKVG
                     ASAKAAAADANRFAKLAQAKGASWAGDVGKLLVDIGNEKNRAAIEQVCKVVLASPAAI
                     AHKQWPALVTKALKLSKTYGDVTEATYGERLTVAQMENDEGGAMDYYADDHLYTFDWL
                     LLTLATPAAIANPAWPGLVLALCEAKAKAVGYYSFGDDEVCALLAVPEATQHPRFGEL
                     VLAARAAFPFASCFGANGALRDYQMRLKSPRASTVVNWPPEPPRPAQPAAPEKAPAKN
                     RPRKAPEKKGSTKAPAKKGPTKAPTNPARAPKRVPAKKPAKAKRSARAAKRR"
     gene            1104..3302
                     /locus_tag="VFV99_19955"
     CDS             1104..3302
                     /locus_tag="VFV99_19955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5061656.1"
                     /translation="MTHRHAWLAALLALAACGKDKDTSSAEAAPAPGGATAAKQPAKA
                     PVKAAARGPEHAVYSLVDSRLSGHLLRNGGLVVPGGSAGFAKYVRFGNLMKGGKRTWE
                     LRQQEGDIKVARITGKSGTVFVPLTAAQAGRNTLRFRLFAQDAGAVSVRVNENKDLNG
                     QLTKGWSTVELTVPAGQLKEGENSIAIFTKSSGSELAWLQVGATAAVGEDGAVKFYEP
                     GSKSLTLPKDGGMSWFVAIPEKAKLTGDLSDGACTINVLATAEDGATLEGKLTGIGSA
                     IDLGAMAGKAARLDLEPTGCAEAQLASAAIVVPGAAPTVKRADPPKYVVFVIMDSLRA
                     DRIRVWNPKARPEVPNWEKLAETSSVFLTHYVQGNESQVSHASMWSSEYLAKHKASEM
                     KDKLDAKLMTIEKVAKKANKYAAGASANGYIRPERGFGNAWDQFVNHITKSLGLKGAD
                     VVDKGISFITPKKDQPWFLYLGLIDTHVTWRAKSPWMEKYDGGYKGKFETSYGDDGPG
                     GSNGKGLSEKEQNHVRALYDSNVSYQDDLLGKLVEQLQTWGIYDKTMIIITADHGDEQ
                     WEENDRVGHGGSQRETLVHVPMLIHYPPLFPATKVHGGTEGIDIVPTLADALGVAFDP
                     EWQGTSLLPLAQGNDAYPLLAMSSAYENMHAGRIGHWKLQLKGGGAPRLWNLAKDPDE
                     RNDLWGKSHIGARLLLDPMWILRGWNVEWKKSQWGNAASVSSRFASDLGE"
     gene            3311..3616
                     /locus_tag="VFV99_19960"
     CDS             3311..3616
                     /locus_tag="VFV99_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061657.1"
                     /translation="MADALTPTEGARFLLERVTDDGACARYRTAIYTPGATYESFATL
                     RDDGTVEIEPSTAPEELSSTLEMLAKLTARSAAKKREDGLPPWPARVLRWRGPGRGE"
     gene            3631..4347
                     /locus_tag="VFV99_19965"
     CDS             3631..4347
                     /locus_tag="VFV99_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061658.1"
                     /translation="MNETAGMLEARLRLIDRIRRVEHQLARDEQAKAAATLMQGRSHS
                     LGNHIQIVKLSSLELERRAQGRDDLVELLTDMRHAAEQATVVLADMFAAARPAERTDV
                     GPVVTHTVRAAVDAARPAFAGDIELRIDLDDTVQTHSTAEELEAIMLAAMLDAVHATR
                     MTIVVRERVVEGKRLVEILRIDNRQQFGDGDLAYMFEPPSLLHLVVTAAKQANGDASI
                     APGRGGLELAIELPVAAKPL"
     gene            complement(4410..5213)
                     /locus_tag="VFV99_19970"
     CDS             complement(4410..5213)
                     /locus_tag="VFV99_19970"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5061659.1"
                     /translation="MPSTRALIHAPWSASKVSTALRCPRLFHFKYIEKLKEPEVMPET
                     RIGKSIHKALELVLMGTPIAEAKQKVREELPNETEQARFDNLGIGIEPFSTRIQQFRR
                     RRRVARQLVEYSLAIREDLSTTQFYSGDAYYRGVIDLGYIFEDDSIALVDHKTGARIP
                     GTSIADQLEGYAVLAAAAFRHVRSFWLGLHWIAERAVEWSKPVRTESVVDEMVPDLLD
                     NIEAAALAVSDGPRPNPGSYCDRCSYRDLCPASKESRYEPVEYYEDDED"
     gene            7253..7699
                     /locus_tag="VFV99_19975"
     CDS             7253..7699
                     /locus_tag="VFV99_19975"
                     /inference="COORDINATES: protein motif:HMM:NF025000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX5061660.1"
                     /translation="MRVLSAISTRRLALRLQRMPAECRRGRPRSRSRRDRLLITCAAL
                     RTNLTIRELAAVFAISKSTAHRIVAAITRRLSCLGDPVCADRRWSWIVDGTLIPTRDH
                     TYAARAKNYRWSCNAQLLVRRRGLRVVAISAGGQAIATIRCTTEDR"
     gene            8005..8466
                     /locus_tag="VFV99_19980"
     CDS             8005..8466
                     /locus_tag="VFV99_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061661.1"
                     /translation="MRRGRCWGCYERWVETRPVGLGARCVTCNEKRRRFLKTVELFGA
                     WKPMCFNCHGCIGALIPMPKTITQLQEAVSRERRKRDRRWGKPDSRVFVYERRVGERR
                     TGREAELPPVDDDMIIEITVDIDMSADDVDFDDITAIRELVAELRPAELAG"
BASE COUNT         1511 a         2855 c         2921 g         1318 t
ORIGIN      
        1 gacgcgcggc aaggtcgaca agaagatcgg cgccgccacg ctcgagatgc tgcgccttgc
       61 acagccacgg gtcgacggac cggcggccgc ggaaatcgca gcgctgatcg ccgagctcga
      121 acagaaagtc ggtgcatccg ccaaggcggc cgccgcggac gcgaaccggt tcgcaaagct
      181 cgcgcaggcg aagggcgcga gctgggctgg cgatgtcggc aagctactcg tcgacatcgg
      241 caacgagaag aaccgcgccg cgatcgagca ggtgtgcaag gtcgtgctcg cgtcgccggc
      301 tgcgatcgcg cacaagcaat ggccggcgct cgtcaccaag gcactgaagc tgtcgaagac
      361 ctacggcgac gtgaccgagg cgacgtacgg cgagcggctc accgtcgcgc agatggagaa
      421 cgatgaaggc ggcgcgatgg actactacgc cgacgatcat ctctacacct tcgactggct
      481 cctgctcacg ctcgcgacgc ccgcggcgat cgcgaatccc gcgtggcccg gcctcgtgct
      541 cgcgctgtgc gaggcgaagg caaaggccgt cggctactac agcttcggcg acgacgaggt
      601 ctgcgcattg ctcgcggtgc ccgaagcgac gcagcatccg cgctttggcg agctcgtgtt
      661 ggcggctcgc gcggcctttc cgttcgcgag ctgcttcggt gccaatggcg cgctgcgcga
      721 ctaccagatg cgtctgaagt cgccacgcgc cagcacggtc gtgaattggc cgccggaacc
      781 gccgcgaccg gcgcaacccg ccgcgccgga gaaggcgccc gcgaagaacc ggccgaggaa
      841 ggcgcccgag aagaagggat cgacgaaggc gccggcgaag aagggaccga cgaaggcgcc
      901 gacgaacccg gcaagagcgc cgaagcgggt tcctgcgaag aaacccgcga aagcgaagag
      961 atcagcccga gcggccaagc gccgctagct cagcgatcca ccgagtgcaa ggcgcttttg
     1021 acgccgcgat ctgtcgacgg cggcggctgc gttcttgacc tcgccgcgga tctgaaatac
     1081 cgacgagcat cggggtattt gagatgactc atcgtcacgc ctggctcgct gctctcctcg
     1141 cgctcgccgc ttgcggcaaa gacaaagaca cgtcctccgc cgaggccgcg cctgcccccg
     1201 gtggtgccac cgccgcgaaa caaccggcga aggcaccggt gaaagcggcc gcgcgtggtc
     1261 ccgagcacgc ggtctattcg ctcgtcgaca gccggctatc ggggcacctg ctccgcaacg
     1321 gcggcctcgt cgtccctggc ggctcggcgg gctttgcgaa gtacgtgcga ttcggcaatc
     1381 tgatgaaggg cggcaagcgg acctgggagc tgcgccagca agagggcgac atcaaggtcg
     1441 cgcgcatcac cggcaagagt ggcaccgtgt tcgtgccgct caccgcagcg caggccgggc
     1501 gcaacacgct gcgcttccgc ctgttcgcgc aggacgccgg cgcggtcagc gttcgcgtca
     1561 acgagaacaa ggacctcaac ggccagctca cgaagggctg gtcgacggtc gagctgacgg
     1621 tccccgccgg gcagctcaaa gaaggtgaga actcgatcgc gatcttcacg aagtcgtcgg
     1681 gctccgagct cgcgtggctg caggtcggtg caaccgccgc ggttggcgag gacggcgcgg
     1741 tgaagttcta cgagcccggt agcaagagcc tcacgctgcc caaggacggc ggcatgtcgt
     1801 ggttcgtcgc catcccggag aaggcgaagt tgaccggcga cctttccgac ggtgcatgca
     1861 cgatcaacgt gctcgcgacc gccgaggatg gcgcgacgct cgagggcaag ctcaccggca
     1921 tcggctcggc catcgatctc ggcgcgatgg cgggcaaggc cgcgcgtctc gatctcgaac
     1981 cgaccggctg tgccgaggcg cagctcgcga gcgccgcgat cgtcgtgccc ggcgcggcac
     2041 ccacggtgaa acgcgccgat ccgccgaagt acgtggtgtt cgtgatcatg gactcgctgc
     2101 gcgcggaccg catccgcgtg tggaacccca aggcgcgtcc agaggtaccg aactgggaga
     2161 agctcgccga gacatcgtcg gtgttcctca cgcactacgt gcagggcaac gagtcgcagg
     2221 tctcgcacgc gtcgatgtgg agctccgaat acctggccaa gcacaaggcg tcggagatga
     2281 aggacaagct cgacgccaag ctcatgacga tcgagaaggt cgcgaagaaa gcgaacaagt
     2341 atgcggccgg cgcgtcggcg aacggctaca tccgcccgga gcgcggcttc ggcaacgcgt
     2401 gggatcagtt cgtcaaccac atcaccaaga gcctcggcct caagggcgcc gacgtggtgg
     2461 acaagggcat ctcgttcatc acgcccaaga aggaccagcc gtggttcctt tatctcggcc
     2521 tcatcgatac gcacgtcacg tggcgcgcga agtcgccgtg gatggagaag tacgacggcg
     2581 gctacaaggg caagttcgag acctcgtacg gcgacgacgg cccgggcggc agcaacggca
     2641 aggggctctc cgagaaggag cagaaccacg tccgcgcgct ctacgactcg aacgtcagct
     2701 atcaggacga cttgctcggc aagctcgtcg agcagctgca gacgtggggc atctacgaca
     2761 agacgatgat catcatcacg gccgaccacg gcgacgagca gtgggaggag aacgatcgcg
     2821 tgggtcacgg cggcagccag cgcgagaccc tcgtgcacgt gccgatgctg atccattacc
     2881 cgccgctgtt cccggcgacg aaggtccacg gcggcaccga gggcatcgac atcgtgccga
     2941 cgctcgctga cgcgctcggc gtcgcgttcg accccgagtg gcagggcacg tcgctgttgc
     3001 cgctcgcgca aggcaacgat gcgtatccgc tgctcgcgat gtcgagcgcg tatgaaaaca
     3061 tgcacgccgg ccgcatcggc cactggaagc tgcagctcaa gggcggcggt gcgccgcggc
     3121 tgtggaacct cgcgaaggat cccgacgagc gcaacgacct ctggggcaag tcgcacatcg
     3181 gcgcgcgcct cctgctcgat ccgatgtgga tcctccgcgg ctggaacgtc gagtggaaga
     3241 agtcacaatg gggcaacgcg gcgtcggtct cgagccggtt cgcgtctgac ctcggcgaat
     3301 agctcagaac gtggctgacg cgctcacgcc gaccgaaggc gctcgctttc tgctcgaacg
     3361 cgtaaccgat gatggcgcgt gtgctcgcta tcgcaccgcg atctacacgc ccggtgcgac
     3421 ctacgaaagt ttcgcaacgc tgcgcgacga cggcaccgtc gagatcgaac catcaacggc
     3481 gcccgaggag ttatcctcga cgctggagat gctggccaag ctgactgcac gcagtgctgc
     3541 gaagaagcga gaggacggcc taccgccgtg gcccgcgcgc gtgctgcgtt ggcgcgggcc
     3601 aggacgcgga gaatgatcca gagtgtcgaa atgaacgaga cggcggggat gctcgaggca
     3661 cggctgcggc tgatcgatcg gattcgccgc gtggagcatc aactcgcgcg cgacgagcaa
     3721 gcgaaggccg ctgccacgct gatgcagggt cgctcgcatt cgctcggtaa tcacatccag
     3781 atcgtgaagc tgtcgagcct cgagctcgag cgccgcgcgc aaggccgcga tgacctcgtc
     3841 gagctgctca ccgacatgcg ccacgccgcc gagcaggcga ccgtggtgct cgccgacatg
     3901 ttcgcggccg cgcggcctgc cgagcgcacc gacgtcggcc cggtcgtgac gcacaccgtg
     3961 cgcgcggccg ttgatgctgc gcgacccgca ttcgccggcg acatcgagtt gcgcatcgat
     4021 ctcgacgaca ccgtgcaaac gcacagcacc gccgaggagc tcgaagcgat catgctcgcg
     4081 gcgatgctcg acgcggtgca cgcgacgcgc atgacgatcg tcgtgcgcga gcgagtagta
     4141 gagggcaagc gcctcgtcga gatcctgcgc atcgacaatc gccagcagtt cggcgacggc
     4201 gacctcgcgt acatgttcga gccgccctcg ttgctccatc tcgtcgtgac ggccgcgaag
     4261 caagcgaatg gcgacgcgtc gatcgcgcca ggccgcggcg gcctcgagct cgcgatcgag
     4321 ctgccggtcg cggcgaagcc gctctagtct tcggagtcga ttccccgtgc acgcgacacg
     4381 agctcgtgta cgaggccgct tgcgctcggc tagtcttcgt cgtcctcgta gtactcgacc
     4441 ggctcgtagc gcgattcctt gctcgccgga cacaagtcgc gatagctgca gcgatcgcag
     4501 tacgagccag gattgggccg cggcccgtcg gagacggcga gtgcggcggc ctcgatgttg
     4561 tcgagcaggt cgggcaccat ctcgtcgacg acgctctcgg tacgcaccgg cttgctccac
     4621 tcgaccgcgc gctcggcgat ccagtgcagg ccgagccaga acgagcgaac gtggcgaaac
     4681 gccgccgccg cgagcaccgc gtacccctcg agctgatcgg cgatcgacgt gccgggaatc
     4741 cgcgcgccgg tcttgtgatc gacgagcgcg atcgagtcat cctcgaagat gtaaccgagg
     4801 tcgatcacgc cgcggtagta cgcgtcgccc gagtagaact gcgtcgtcga caggtcctcg
     4861 cggatcgcga gcgagtactc gacgagctgg cgcgcgaccc gccgccggcg acggaactgc
     4921 tggatgcggg tcgagaacgg ctcgatgccg atgccgaggt tatcgaagcg cgcctgttcg
     4981 gtctcgttcg gcagctcctc gcggaccttc tgcttggcct ccgcaattgg cgtgcccatc
     5041 agcacgagct cgagcgcctt gtggatcgat ttgccgatgc gcgtttccgg catcacctcg
     5101 ggctctttga gcttctcgat gtacttgaag tgaaacaagc gcggacagcg cagcgcggtc
     5161 gagaccttgg aggccgacca cggggcgtgg atcaacgcgc gtgtcgacgg cacgggccct
     5221 cagactaaga gctgtctcgt aatacgtcca gcgaggaacg tacgtcgtgg acaggttggt
     5281 gcgcacgcgt gcccggtcga acgtggtctg gctcgcgcca ggctcatttc gggttgggca
     5341 cgatgccatg ccaacggttc gccgagccac ccaggcagag ccgggtgtgg acgtcgacca
     5401 cgcgatgaac gatccatcgc gggccgagag gtcggttgac tcggacaggg tgtcttgcca
     5461 cccctggtac aaagaactcg taagcacgcg ccggatctcg gcgagcgcgt cggccgacgc
     5521 gtcgtagtcg acacgtgctg acgtcggatt gccgcgctga agcagcacga caaacgatgc
     5581 aagcacggcg gcgactgcat cgcgatcgcc gtacagctgg cacgccgagc agcgatggat
     5641 ccaattcgcg aggcgcttgt cgacgtgagc accgcagggg cagtgttgcg acagtgcggt
     5701 cgttcgcgtg gacacgcgcg caacgccgcc gttggtagca ccggcgagcg tggccaccgc
     5761 cttcgcctcg cgatcgatcg ccgcgacgag cgtggccggc gagaacgccg cgacggcgcg
     5821 gccccacgac gccgaccacg cggaaatgct gcagtcttcc acgacgagct gatagccgtg
     5881 cgtggcgacg acctcgctgg cgacctgccg ggcgcgctcg cggcgcgcct gcgtggccgc
     5941 ctcggcctct gccgcctgtg cagcacgcag gcggcggaag ctcgccgata gctggtcgcg
     6001 cttgaagctc tggagcggca cgccgtccgc gcgcgccttg cggcgcccca tcgggatgac
     6061 ctcgacgggg ccaagccctc tggcttcgcg ccgccgcgcc tgcttctctt gccgcttgga
     6121 cagctgatac tgcgcgcggt tggccgcgcg acgcgagcgc tcgagctcgc gctggcgacg
     6181 gcgctcacgc cgcctccgtc cacggtcgcg ctgcttcgtg ggctcgtcac gctcgattcg
     6241 cgataggcgc atcgagcgcc cggtgtcgtg cgaggcgatc gagatgttgg agacgttgac
     6301 gtcgatgccc gccgtgcgat cgatcgtgtc gatcgcgacc ttcgcgcggc gcgccgccac
     6361 cgcgggcgac acgtacggcg ccgtccacac catgaggtgg gcctcgtagc gccagccgcc
     6421 cgccacgaac ggatcctggt ggcggaccag atcgatcttg tgccacttcg tcggatcggc
     6481 aaggtgatga tcgaggatgg gctggttgca cggcggcgtc ggcaggcgca ccggaagcac
     6541 gagcgggccg ccggcaagac cactgaacac gaccgcgagc ggcccggtat agctccacca
     6601 cgcgtcggcc tcgacgggcc ggagccggcg tggctggacg aagtcgccgt cgcggtcggt
     6661 atatgcggcg cgatggccgt cgagcgagcc atggaggcga aatgtctccc acttgcggtc
     6721 tttggtatgc gagcgagcac gtccgggcag ccgcgtgaaa tcaaaccagc ggccgacgtg
     6781 tggcgcgccc tgccgcttgc cggtcttgtc gcgaaacagg tggcgctcgg tgcccgtcca
     6841 cactgaatcg gcgatgtgca tggccagcgc tttggtcacg aaccgccgca ggtgcggcgc
     6901 ggcattcagg tggtcagagg ccgcacgctc gagcgccttc cgcgacaggc ccaagcgctc
     6961 gcgcaccgcc gcgggagcct tggcgcgctc gtgcggtgcg gcccagtacg cgcgacaccg
     7021 gtcgcgggca tcgcgctgga gcgctcgctt gagcgagtag gcgtcctgaa acatctgctc
     7081 gatacgcgcg cgctggagcg gatcgctggt atcgagcgcg agccgcagca ccgacacggg
     7141 ggcatcagga cgtggcgacc acggcgtctg tttgttcgga ccacgcgtcc gctttcccga
     7201 cgactccacc gatcgatcgt acgtcacgct tctgacaatg acgatcccac gcatgcgggt
     7261 tctctctgcg atcagcacgc gtcggctcgc gctccgactg cagcgcatgc cagccgagtg
     7321 ccggcgcggc cggccgcggt cgcgctcgcg tcgcgatcgc ctcctcatca cgtgtgccgc
     7381 actacgcacc aacctcacga tccgcgagct cgcggcagtg tttgcgatct cgaagtccac
     7441 tgcgcatcgg atcgtcgccg cgatcacgcg ccggctctcc tgtctaggtg acccggtttg
     7501 tgccgatcga cgctggtcgt ggatcgtcga cggcacgctg attccgacgc gggaccacac
     7561 gtacgcggcg cgcgcaaaga actatcgctg gtcgtgcaac gcgcagctgc tcgtacgccg
     7621 tcgcggtctc cgagtcgtcg cgatctccgc gggtggccag gcaatcgcaa cgatccgatg
     7681 cactaccgag gatcggtgag agagcctcta tgtgaggcac acggcagtgt gttggcagat
     7741 ggcggctatc gcggcatccc tgagcccgtg acacccagat ttgaaggccg ccgcatcgtg
     7801 cgtgaccgcg catggcggtg atatcgccgc ggcacacacg cgtcgaacac gacacgcttc
     7861 gcgtcgccta tctccacaat cttcaactcg acttgcggga caaccctagc cgaaaacttg
     7921 cgaccgggag agcgttcgac ctaccaccga agggtggaca ctcctgcgcg cgtttgcgaa
     7981 ctctgccgtg cacacgtctc cgaattgcgc cgtggccgct gttggggctg ctacgaacgc
     8041 tgggtcgaga cgcggccggt cggcctcggc gcccgctgcg tgacctgtaa cgagaaacgc
     8101 cgacgcttcc tcaagaccgt cgagctgttc ggcgcctgga agccgatgtg cttcaactgc
     8161 catggctgca tcggtgcgct gattccgatg cccaagacca tcacgcagct ccaggaagcg
     8221 gtgtcccgcg agcgccgcaa gcgcgaccgc cgctggggca agcccgacag ccgcgtgttc
     8281 gtctacgagc gccgcgtcgg cgagcgccgc accggccgtg aggccgagct acctccggtc
     8341 gacgacgaca tgatcatcga gatcacggtc gacatcgaca tgtcggccga tgacgtcgac
     8401 ttcgacgaca tcaccgcgat tcgcgagctg gtcgccgagc tgcggccggc cgagctcgcg
     8461 ggctgatcaa cacgtaagcg taagcgcaag cgtaagcgga acgccgccgg taggaatcaa
     8521 ggttgttgtc gcgtgagtct taatgcggct ggccggtttt cggcgatctg atccgatctg
     8581 gtcagactga tcaggtcctc gggga
//
LOCUS       DASUFX010000498         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1747190, whole genome shotgun sequence.
ACCESSION   DASUFX010000498 DASUFX010000000
VERSION     DASUFX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747190"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VFV99_19985"
     CDS             complement(<1..1526)
                     /locus_tag="VFV99_19985"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HEX5061662.1"
                     /translation="MMRRLGVVVALLCPTPALATEAGLDVGRIVDNATLHHNNESVDS
                     TGTPWIRLNLRLDDWSAPDDDTKRGPDQLTWYETYDRIVDDYLTRGIQVYALINDEAV
                     YSTQPYGSDAWIADYVQNAVKIVDHFKNRIRVYEIINEPNDWAGGSTSRFSPRAYAKI
                     LQDTYLAVKHDAGHIDDRCWQVQLVSGPLFSFDDNSSAEYLQQAYSIGKSQLAWDYTH
                     AETGSYPLDGIGYHMYVAQGLDSSSADVRTQMLASLGAMWNVVTSYEGTGTGKRIWVS
                     EYGWEAGVVGDAVQAERMQAGFSAMNEFGKVALALYFNFQDFPGASYGVFDAANQPRP
                     SATTMKSLAPPTRRARIVGINMPALAPGALGDATIILENRGTTTWTDEFRLASAPGCP
                     EAATANAIAWEPTAGYSNGILDARVLMPHAVGPGETIEIHVPVRAPAEPGEYAFAARM
                     VHEGVEWFGPTVSAVVTVAAGNGSGDGSGSGSDDGDHTASGGGCSTGHGSSVWLVVAL
                     G"
     gene            1675..3222
                     /locus_tag="VFV99_19990"
     CDS             1675..3222
                     /locus_tag="VFV99_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061663.1"
                     /translation="MRTVALLAQLGLVACADPAQPPASCECTDGKICEGDRCVDPWRY
                     GAPAFSTCPNESRATPESLAQKAAIYDARAVALHTAPAHPWVIDVMIAPGVDPETATT
                     ADVVLWRSGENDGLFSGLQLAAEAYRYAVTHDPAARDALALLLHGEQQRMAITGVPGL
                     FTRQLIPPGVAGLACPTDPAAYVPSPDKRGNKWVRIGADGCAQTADASGTFTATTHCG
                     LAQFANWCFLDNISQDEYVGHIFALGAIARVVDDAELRLAALDMLQQIGDHLRANDMR
                     FVDWDGRPTQWGKLYPGAPGDTPGYLAVLGSSMIANAALADPTLEPFARATATDYAAA
                     LDQLDLWEGPDGCESNWNNISMLLAMFHHLLWTRDDVATWRNAFVHEVVEAPVTRGPL
                     AQHNAWYDIMWAAQKPLGPNTDGPAYAAVEDAVCQLRQFPRSNHIVARDTTVLAPDAC
                     NDRFDRSLAATPFQIADRCAATYAWWGNPYERTMCGDDPTLVQQPAGYLLPYWMGRYY
                     GFISAAQ"
     gene            complement(3239..3919)
                     /locus_tag="VFV99_19995"
     CDS             complement(3239..3919)
                     /locus_tag="VFV99_19995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061664.1"
                     /translation="MTDRPIELAKNRAAHLLAAADADLEAGAIDEAEWYRRVGAAITP
                     SYLAADTPWAQSGKSGDAASFERARRLLVDAMRPGCFLDVGCASGYLMECMQTWCAEA
                     GIACEPYGLDIEPALAELARSRLPHWRDRIFTGNAIDWQPPQRFDVVRTGLEYVPTRR
                     QRDFVTRLLADVVADGGRLVIGVFSEERDDHAMERQLETWGFAVTGRSQRLHADPRLA
                     YRVVWIDR"
BASE COUNT          719 a         1590 c         1503 g          753 t
ORIGIN      
        1 ccgagtgcaa cgacgagcca cacgctactg ccgtgacccg tcgaacaccc gccaccgctg
       61 gccgtgtgat cgccatcgtc gctgccgctg cccgaaccgt cgcctgaccc attacctgct
      121 gcaaccgtga cgacggcgct caccgtcggg ccgaaccatt cgacgccttc atgcaccatg
      181 cgcgcggcga acgcgtactc gccgggctcg gcgggcgcgc gcaccggcac gtggatctcg
      241 atggtctcgc cgggcccgac tgcatgcggc atcagcacgc gcgcgtcgag gatgccgttc
      301 gagtaaccgg cggttggctc ccacgcgatc gcgttcgcag tcgctgcctc cgggcagccg
      361 ggcgcgctcg cgagccgaaa ctcgtcggtc cacgtcgtcg tgccgcggtt ctcgaggatg
      421 atcgtcgcgt cgccgagggc gccgggtgcg agcgccggca tgttgatgcc gacgatccgt
      481 gcgcgtcgcg tcggtggtgc gagcgacttc atcgtcgttg cggacggtcg cggctggttc
      541 gcagcgtcga acacgccgta gctcgcgccc gggaagtcct ggaagttgaa gtagagcgcg
      601 agtgcgacct tgccaaactc gttcatcgcc gagaagccag cctgcatgcg ctccgcttgc
      661 accgcgtcgc cgacgacgcc cgcctcccat ccgtactcgc tgacccagat ccgcttgccg
      721 gtgccggtgc cttcgtagct cgtcaccacg ttccacatcg ccccaaggct cgcgagcatc
      781 tgcgtgcgca cgtccgcact cgacgagtcg agaccctgcg cgacgtacat gtgatagccg
      841 atgccgtcga gcggatagct gccggtctcc gcgtgcgtgt agtcccacgc gagctggctc
      901 ttaccgatcg agtacgcctg ctgtagatac tccgcgctcg agttgtcgtc gaacgagaac
      961 agcggccccg atacgagctg cacctgccag cagcgatcgt cgatgtgacc ggcgtcgtgc
     1021 ttcaccgcga ggtacgtgtc ctggaggatc ttcgcgtacg cgcgcggcga aaaccgcgac
     1081 gtcgagccac cggcccagtc gttcggctcg ttgatgatct cgtagacgcg gatccgattc
     1141 ttgaagtgat cgacgatctt cactgcgttc tgcacgtagt ccgcgatcca cgcgtcgcta
     1201 ccgtacggct gcgtcgagta caccgcctcg tcgttgatca gcgcgtagac ctggatgcca
     1261 cgcgtgaggt agtcgtcgac gatccggtcg taggtctcgt accaggtcag ctgatctggc
     1321 ccgcgcttcg tgtcgtcatc gggagcgctc cagtcgtcga ggcgcaggtt gagccggatc
     1381 cacggcgtgc cggtcgagtc gacgctttcg ttgttgtggt gcagcgtcgc gttatcgacg
     1441 atgcgcccga cgtccaagcc tgcttcagtc gccagtgcgg gcgtcgggca tagcagggcc
     1501 acgacgaccc cgagtcgtct catcatcctc atcgcgagcg tacacgtgca attggtcggc
     1561 ccacagacaa gcccgcggaa tttcgtgcac gcgtgatcgc gagtcgatca cggcgctgcg
     1621 cagtgcaaac gatcggtaac actcggccgc gtgcagctgg agtagggtgg cccggtgcga
     1681 acggtcgctc tgctcgctca gctcggtctt gtcgcgtgcg cggaccccgc gcagccgccg
     1741 gcatcgtgcg agtgcaccga cgggaaaatc tgtgagggcg atcgctgtgt cgatccatgg
     1801 cgctacggcg cgcccgcatt ctcgacctgc ccgaacgagt cccgcgcaac gccggagtcg
     1861 ctcgcgcaga aggcggcgat ctacgacgct cgcgccgtcg cgctgcacac ggcacccgcg
     1921 catccgtggg tgatcgacgt catgatcgcg cccggcgtcg atccggagac cgcgacgacc
     1981 gccgacgtcg tgctctggcg cagcggcgag aacgacggcc tgttcagtgg tctccagctc
     2041 gcggccgaag cgtatcgcta cgcggtcacg cacgaccccg cggcgcgcga cgcgctcgca
     2101 ctgctgctcc acggcgaaca gcagcggatg gcgatcaccg gtgtgccggg tctgttcacc
     2161 cggcagctga ttccgcccgg cgtcgccggc ctcgcgtgcc cgaccgaccc agctgcctac
     2221 gtcccatcgc ccgacaagcg cggcaacaag tgggtgcgga tcggcgccga cggttgtgcg
     2281 caaaccgccg acgcgagcgg cacgttcacc gccaccacgc actgcggcct cgcccagttc
     2341 gcgaactggt gcttcctcga caacatctcg caggacgaat acgtcggtca catcttcgcg
     2401 ctcggcgcca tcgcccgcgt cgtcgacgat gccgagctcc ggctcgccgc gctcgacatg
     2461 ctccagcaga tcggcgacca cctgcgcgcg aacgacatgc ggttcgtcga ctgggacggc
     2521 cgcccgaccc agtggggcaa gctctatccc ggtgcgccgg gcgacacgcc gggctacctc
     2581 gccgtgctcg gctcgagcat gatcgcgaac gcagcgctcg ccgatccgac gctcgaacca
     2641 ttcgcgcgcg cgacggccac cgactacgcc gccgcgctcg atcagctcga tctatgggag
     2701 ggccccgacg gctgcgagtc gaactggaac aacatctcga tgctgctcgc gatgtttcat
     2761 cacctgctgt ggacgcgcga cgacgtcgcg acgtggcgca acgcattcgt gcacgaagtc
     2821 gtcgaagcac cggtcactcg tggaccgctc gcgcagcaca acgcctggta cgacatcatg
     2881 tgggccgcgc aaaagccgct cggcccgaac accgacggtc cggcgtacgc cgcggtcgaa
     2941 gacgcggtct gccagctccg ccagttcccg cgcagcaatc acatcgtcgc gcgcgacacg
     3001 acggtgctgg cgccagatgc gtgcaacgat cgcttcgacc gctcgctggc cgcgacgccg
     3061 tttcagatcg cggaccgctg tgcggccacc tacgcgtggt gggggaatcc gtacgagcgc
     3121 acgatgtgcg gcgacgatcc aacgctcgtg cagcaaccgg ccggctatct acttccctat
     3181 tggatgggcc ggtactacgg gtttataagc gccgcgcagt gatgtggttc gcgacgcgct
     3241 agcgatcgat ccacacgacg cgatacgcga gtcgtggatc ggcatgtagc cgctggctgc
     3301 ggcccgtgac ggcgaagccc caggtctcga gctggcgctc catcgcgtgg tcgtcgcgct
     3361 cttcgctgaa cacgccgatc acgagccggc caccgtcggc gacgacgtcg gcgagtagtc
     3421 gggtgacgaa gtcgcgctgc cgtcgcgtcg gcacgtattc gagtcccgtc cggacaacgt
     3481 cgaagcgctg cggcggctgc cagtcgatcg cgttgcccgt gaagatgcgg tcgcgccagt
     3541 gcggcaaacg gctgcgcgcg agctccgcga gcgcgggctc gatgtcgaga ccgtagggct
     3601 cgcacgcgat gccggcctcg gcgcaccacg tctgcatgca ttccatcagg tagccgctcg
     3661 cgcagccgac atcgaggaag cagccggggc gcatcgcgtc gacgagcaag cgacgtgcgc
     3721 gctcgaagct cgcagcgtca ccactcttgc cggactgtgc ccacggcgtg tcggccgcaa
     3781 ggtagctcgg cgtgatcgcc gcgcctacgc gccggtacca ctcggcctcg tcgatcgcgc
     3841 cggcctcgag atcggcgtcg gcggccgcga gcagatgcgc ggcgcggttc ttcgcgagct
     3901 cgatcggccg gtcggtcacc cgcgaagttg tagcgccgtc tgacccacgt gggtcaactc
     3961 cctcgcgtgc ggcgctggct cggcgaggaa cgcgcggcgc gggttgcggg gctatcggcg
     4021 atcgtcacgg ggagcgacgt ctcctaccca cgacggtcaa cgagctcgac ggcggagttc
     4081 gcttcggaga gacggacccc gagctgaaag caaagttgcc ggcgatagcg gcgtctgacc
     4141 cacgtgggtc aaccaagaac gcgaaccggc cctgtagatc tcgcgctcgg tggctgcgtc
     4201 acttgtgacc cacgtcggtc aaccaagaac gcgaagcggg ctcgtagatc tagcgctcgg
     4261 tggctgagtc accgcggacg tacgtgggtc aaccaacgcg aagcgggctt tgtagatctc
     4321 gcgctcggtg gctgcgtcac ctcggacaac ctcagaacgc gaacgcggcg ctcgctcggg
     4381 catctggcgc tctgcaggta cgcgacctcc cggcgaacga atccaccgcg ctgtcgcgtc
     4441 gcgtccgggc gtgggcggtc tactcgtaca cgacgcccga agcgagcttc gggctgccga
     4501 gatctcgcgc gtcgtgcagc gacgctcctc ccgggagggt ctctttgatc gacgtcgcga
     4561 gaccg
//
LOCUS       DASUFX010000499         7709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1329782, whole genome shotgun sequence.
ACCESSION   DASUFX010000499 DASUFX010000000
VERSION     DASUFX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329782"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFV99_20000"
     CDS             complement(<1..443)
                     /locus_tag="VFV99_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061665.1"
                     /translation="MADTGSGVRRADATTIVTFPFRYGLVAHHVYALAEIEMGIIADG
                     APRIVRPARMSSGAARSGMGQETVDTVAADMSVAGYLVAGVRGDVGAVALSAELAAGG
                     RGYNNSAEQLGGPWRGLVEGRVRGERMVTEHVGIGAWVGTSILGSR"
     gene            604..1104
                     /locus_tag="VFV99_20005"
     CDS             604..1104
                     /locus_tag="VFV99_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061666.1"
                     /translation="MSGFQLKTISPDGIPHALEKAERYRLLNDPAQAESICRDVLATD
                     GDNQDALRMLILALTDQFGAHAAAGAAREARTHINALKDEYERAYYTGIVFERETRAY
                     LERKNVVRSAAYDGFRHAMEWYERAEALRPPGNVDAVLRWNSCVRALERERLEPESRE
                     PELPLE"
     gene            1107..1595
                     /locus_tag="VFV99_20010"
     CDS             1107..1595
                     /locus_tag="VFV99_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061667.1"
                     /translation="MRQFALLLLLVACAKKDAASGGACKDLTVTVDGTPLPAMPHGLA
                     KANNMNGDISYEVDVFNHDKTTCDMLLDKSGRQIPEGEVSVRAFAAGAGMTGKGVAIS
                     AHTQMGGNVTLASPPPKAVGDVVKVCVDNVSFKPIAGDYKDKQVVVNGRFVGTYCGEM
                     KW"
     gene            complement(1626..2495)
                     /locus_tag="VFV99_20015"
     CDS             complement(1626..2495)
                     /locus_tag="VFV99_20015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5061668.1"
                     /translation="MNNVAVTIEQARALDALERTGTFARAAQSLRRGHTSVLYLIKTL
                     EDALGFAVLDRSGYRTRITAKGLRVLEGCRELLAAEAALATTVTELRAGWEPTVSVVF
                     DGIVPIEPLLRAVGRLVKEKIPTKFEVRAEFLSGVEDAFAQTRADLMIAVLPPRTRDL
                     ATVTLTPLQASLVARADHPLAKGRHDEQALRRELLLTVRGSDPRLDLPTAGLEAHSTV
                     LLNDFGSKRAAILAGIGYGWLPDVLIESELERRRLRRIRWTRSSVHQFHPRLYHRGQP
                     GPAARRLIAALTT"
     gene            2563..3690
                     /locus_tag="VFV99_20020"
     CDS             2563..3690
                     /locus_tag="VFV99_20020"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5061669.1"
                     /translation="MSIPHIRGRVALQAHVALPEGTVEEEYARNGFSGRYAHLYREHA
                     PVGWTRIDGPLRPRLYNVGETPVDDYLASRIALLANADCSIQMAAVREPMPYLFRNAD
                     ADELLFVHAGAGRIETDFGPLAYETSDYLVIPRGTQYRLVPTSVTRLLVIETTGELAL
                     PDRGMLGQHALFDPAMLRVPSPSEPSSLAAGQGGARANEWRVVIKRQGVLTSVYYPHC
                     PLDVVGWKGTLSVWQLNVRDIRPVSSDRYHLPPTAHTTFVAPNVAICTFAPRPLENGD
                     PRALKVPFYHSNIDFDEVLFYHAGEFFSRDGVGAGMLTFHPQGIHHGPQARAAARAAA
                     APRTEEVAVMIDARKPLDICVPAAPYEVEHYWKSWQPEGTR"
     gene            3687..4727
                     /gene="hppD"
                     /locus_tag="VFV99_20025"
     CDS             3687..4727
                     /gene="hppD"
                     /locus_tag="VFV99_20025"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX5061670.1"
                     /translation="MSTETNPCGLRGLAFMILAAPDPEPLHRMLLAFGFSRTMRHRVR
                     PLDLYEQGEIRLLIDRGSSGFNARFAAEHGPCISAMGWRVDDAMLAAAAAVHRGARRG
                     QGDLRHLDGTFVLAVRGIGDSLIYFVDPHDSWATLGFVPLESPDRVRSKGFTRIDHLT
                     NNVPRGELAQWGAFYRDVFGFTEVRYFDIRGVKTGLHSYALRSPCGTFCIPINEGSGT
                     GSQIDEYLDEYKGPGIQHLAFLTDDILGSLRALEDTPLQFLDIDADYYGTVFERVPNV
                     AEDRTEIARRNVLVDGDRDGYLLQIFTKNLIGPIFIELIQRRNHLSFGEGNFGALFRS
                     IERDQQRRGYLT"
     gene            4737..6152
                     /gene="boxB"
                     /locus_tag="VFV99_20030"
     CDS             4737..6152
                     /gene="boxB"
                     /locus_tag="VFV99_20030"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX5061671.1"
                     /translation="MAIDLSAKIPNNVDLDSDPKLRRALEAWQPQYVEWWKEVGPVGF
                     QNADVYLRTAIDVGAQGWANFGYVKMPDYRWGIFLATPEPHRTIGFGDHADAPVWQDV
                     PGEHRAALRRLVVTQADTEPASVEQQRRLGATCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLHKYFGRDGRDEGDELLARRAGSADKPRILETFNEPVEDWLDFFMFTMFTDRDGKFQ
                     LLSLAESAFDPLARSCRFMLTEEAHHLFVGETGIDRIVQRSAEMTRYDRNGDAYRHGA
                     IDLPIIQRYLNLWFSSCLDLFGGEVSSNAANNFAAGLKGRAHESKYHDHDERGDVRFV
                     EVVDDDHVITREIAARLAINEDTRDAYIRDCERAVARWNRTIRNCGLDFELTLPHRRF
                     HRAIGEHAGWCFAPDGRLISVEQFLTHEDEWLPSAADRAKVRALQTRPVTVPGQFASW
                     IAPPLRGVDGRPLDFEYVRLD"
     gene            6152..7504
                     /locus_tag="VFV99_20035"
     CDS             6152..7504
                     /locus_tag="VFV99_20035"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX5061672.1"
                     /translation="MTRRSIIEPFKIKVVEPLPMRTVAEREAILAACGYNLFGVRAAD
                     VTFDFLTDSGTTAMSAAQWGAMMTADESYAGSRSFYRFEDVVRHITGYDHIIPTHQGR
                     AAEHLLFSLHCKRGEVVPSNNHFDTTRANLEQLGVEAVDLVCAEARDPASEVPFKGNI
                     DVPRLEAFLREHRVRVPFVMTTITNNTGGGQPVSLANLRAVSAVCRREGIPFLLDACR
                     FAENAWFIKVREPGQADRTPREIAHEMFALADGCLVSAKKDGLVNIGGFIALRDGAWV
                     PDLRSRLILTEGFPTYGGLAARDLEAIAVGLDEVLDERYLAYREAVTRYVADGLQRAG
                     VPTMRPPGGHAVYLDAKALLPHVPPAQFPAQALAVELFRAEGIRSCEIGSVMFGKLAA
                     LELVRLAIPRRVYTQAHMDYVLEGIAEIAERRATLRGMRIVEEPPFLRHFTARFAPLE
                     "
BASE COUNT         1313 a         2641 c         2530 g         1225 t
ORIGIN      
        1 cgcgagccga ggatgctcgt gccgacccag gcgccgatgc caacgtgctc ggtcaccatc
       61 cgctcgccgc gcacacggcc ttcgacgagg ccgcgccacg gaccaccgag ctgctcggcg
      121 ctgttgttgt acccgcggcc gcccgccgcg agctcggccg acagcgcgac ggcaccgacg
      181 tcgccacgga cacccgcgac gagatagccg gctaccgaca tgtcagctgc caccgtatcc
      241 acagtttcct gtcccatccc cgagcgagcc gcacccgagc tcatgcgcgc cggccgaacg
      301 atgcgaggcg caccgtcggc gatgatgccc atctcgatct cggcgagcgc gtacacatgg
      361 tgcgcgacca ggccgtagcg aaacggaaac gtcacgatcg tggtggcgtc ggcgcgccgt
      421 acgccggaac cggtatctgc catgaagtgc cgcgccacga tgccgcacag ccctgggctc
      481 gaccgattgc acgtctctgc gcgcgcgctg cgggctgcca ggattccaac aatcagcacc
      541 gctgccattc gcatgcgaag gggcgttgca agcgcgatac cgcaacgtgc tagcgtcggg
      601 cccgtgagcg gcttccagct gaagacgatc tcgcccgacg gcattccgca cgcgctcgag
      661 aaggcggaac gctaccggct gctcaacgat ccggcgcagg cggagagcat ctgccgcgac
      721 gtgctcgcga ccgacggcga caaccaggat gcgctgcgca tgctgatcct cgcgctcacc
      781 gaccagttcg gtgcgcacgc cgccgccggt gcggcacgcg aagcacgcac acacatcaac
      841 gcgctgaagg acgagtacga gcgcgcgtat tacacgggca tcgtgttcga gcgtgagacg
      901 cgcgcgtatc tcgagcgcaa gaatgtcgtg cgctcggcgg cgtacgatgg ctttcgccac
      961 gcgatggagt ggtacgagcg cgccgaggcg ctgcgtccgc ctggcaacgt ggacgcggtg
     1021 ctgcgctgga acagctgcgt gcgcgcgctc gagcgcgagc gactcgagcc cgaatcgcgc
     1081 gaacccgagc tgccgctgga gtgacgatgc ggcagttcgc gctcttgctg ctgctcgtcg
     1141 cgtgcgcgaa gaaagacgcc gcaagcggcg gtgcgtgcaa ggacctcacg gtcaccgtcg
     1201 acggcacgcc cttgcccgcg atgcctcacg ggctcgcgaa ggcgaacaac atgaacggcg
     1261 acatctcgta cgaggtcgac gtcttcaatc acgacaagac gacgtgcgac atgctgctcg
     1321 acaagagcgg ccgccagatt cccgagggcg aggtcagcgt gcgcgcgttc gcagccggcg
     1381 ccggcatgac gggcaaaggc gtcgcgatca gcgcgcacac gcagatgggc gggaacgtca
     1441 cgctagcgag cccgccgccg aaagcagtcg gcgatgtcgt gaaggtctgc gtcgacaacg
     1501 tcagcttcaa accgatcgcg ggcgactaca aggacaagca ggtcgtcgtg aatggccggt
     1561 ttgtcggcac gtactgcggc gagatgaagt ggtgagcgta aacagggtct gacccgtttt
     1621 cttcactacg tcgtcagcgc agcgatcagg cggcgcgcgg ccggccccgg ctgtccgcga
     1681 tggtacagac gcggatggaa ttggtggacg ctcgaccgcg tccaccggat gcggcgcagg
     1741 cgccggcgct ctagctccga ttcgatcagc acgtcgggca accagccgta gccgataccg
     1801 gcgaggatcg ctgcgcgctt cgagccaaaa tcgttcagca gcacggtcga gtgcgcttcg
     1861 agcccggcgg tcggcagatc gaggcgcggg tcggaaccgc gcacggtgag cagcagctcg
     1921 cggcgcagcg cctgctcgtc gtggcggcct ttcgccagcg gatgatccgc gcgcgcgacg
     1981 agcgaggcct gcagcggcgt cagcgtgacg gtcgcgaggt cgcgcgtccg cggtggcagc
     2041 accgcgatca tcagatcggc gcgcgtctgc gcgaacgcgt cttccacacc ggacagaaac
     2101 tccgcgcgca cctcgaactt cgtggggatc ttctccttca ccaaacggcc aactgcgcgc
     2161 agcagcggct cgatcggaac gatcccgtcg aacacgaccg aaacggtcgg ctcccagccc
     2221 gcgcgtagct cggtgaccgt ggtcgcgagt gccgcctcgg cggcgaggag ctcgcggcag
     2281 ccttcgagca cgcgcagacc tttcgccgtg attcgggtgc ggtagccaga gcgatcgagg
     2341 accgcaaacc ccagcgcgtc ctcgagcgtc ttgatcagat acagcaccga ggtgtgaccg
     2401 cgccgcagtg attgcgcggc acgcgcaaac gttccggtgc gctcgagcgc atcgagtgcg
     2461 cgagcctgct cgatggtcac cgcaacattg ttcatctctt gaacaatgat agcccaaaca
     2521 tagttctttt tctgcaaggc cgccggagcg caggctgcgg gcatgtccat cccgcacatc
     2581 cgtggccggg tcgcgctgca agcccacgtc gcgttgccag agggaacggt cgaggaggag
     2641 tacgcgcgca acggcttctc cggccgctat gcgcacttgt atcgcgagca cgcgcccgtc
     2701 ggctggacgc gaatcgacgg cccgctgcgc ccgcgcctct acaacgtcgg tgagacgccg
     2761 gtagacgatt atctcgcgag ccgcatcgcg ctcctcgcca acgccgactg cagcatccag
     2821 atggccgcgg tacgcgagcc gatgccatac ctgtttcgca acgcggacgc cgacgagctg
     2881 ttgttcgtgc atgcgggtgc cggccgcatc gagaccgact tcgggccgct cgcgtacgag
     2941 acgagcgact acctcgtgat cccacgcggc acgcagtacc ggctcgtgcc gacgagcgtg
     3001 acgcgcctgc tcgtcatcga gaccactggc gagctcgcgc tgccggatcg cggcatgctt
     3061 ggccagcacg cgctgttcga tcccgccatg ctacgcgtgc cgtcaccatc ggaaccgagc
     3121 tcgctcgcgg ctggccaggg tggcgcgcgc gcgaacgagt ggcgcgtcgt gatcaagcgc
     3181 cagggtgtgt tgaccagcgt ctactacccg cactgcccgc tcgacgtcgt cggctggaag
     3241 ggcacgctct cggtctggca gttgaacgtg cgcgatatcc gtccggtgtc gagcgatcgc
     3301 taccacctgc cgccaacagc acacacgacg ttcgtcgcac cgaacgtcgc gatctgcacg
     3361 ttcgcgccgc ggccgctcga gaacggcgac ccgcgcgcgc tcaaggtccc cttctatcac
     3421 tcgaacatcg acttcgacga agtgctcttc tatcacgcgg gcgaattctt cagtcgcgac
     3481 ggcgtcggcg ccggcatgct gacgttccac ccgcagggca tccaccacgg gccgcaggct
     3541 cgtgccgctg cgcgcgcagc tgccgcgcca cgcaccgagg aagtcgcggt gatgatcgac
     3601 gcccgcaagc cgctcgacat ctgcgtgccg gccgctccgt acgaggtcga gcactactgg
     3661 aagagctggc aaccggaggg cacgcgatga gcaccgagac gaacccgtgt ggcctgcgtg
     3721 gcctcgcgtt catgatcctc gccgcgccgg atcccgagcc gcttcatcgc atgctgctcg
     3781 cgttcggctt ctcgcgcacg atgcgccacc gcgtgcggcc actcgacctc tacgaacagg
     3841 gcgagatccg cctcttgatc gatcgaggca gctccgggtt caacgcgagg ttcgcggcgg
     3901 agcacggccc gtgcatcagc gcgatgggct ggcgcgtcga cgatgcgatg ctcgctgcag
     3961 cggccgctgt gcaccgggga gcgcggcgcg gtcagggcga cttgcggcac ctggacggca
     4021 cgttcgtact cgccgtgcgc ggcatcggcg actcgctgat ctacttcgtc gacccgcacg
     4081 atagctgggc aacgctcggc ttcgttccgc tcgaatcccc cgaccgtgtt cggagcaagg
     4141 ggttcactcg catcgaccac ctgacgaaca acgtgccgcg cggagagcta gcgcagtggg
     4201 gagcgttcta tcgcgacgta ttcgggttta ccgaggtgcg gtacttcgac atccgcggcg
     4261 tgaagacagg cctgcattcg tacgcgttac gctcgccgtg cggcacgttc tgcattccga
     4321 tcaacgaggg tagcgggacc ggcagccaga tcgacgagta cctcgacgag tacaagggac
     4381 cggggatcca gcacctcgcg ttcttgaccg acgacattct cggctcgctg cgtgcgctcg
     4441 aagacacgcc gctgcagttc ctcgacatcg atgctgacta ctacggcacc gtgttcgagc
     4501 gcgtgccgaa cgtcgccgag gaccgcaccg agatcgcacg ccgcaacgtg ctggtcgacg
     4561 gcgaccgcga cggttacttg ctccagattt tcaccaagaa cctgatcggt ccgatcttca
     4621 tcgagctgat ccagcgtcgt aatcacctct cgttcggcga aggcaacttc ggcgctctgt
     4681 tccgctccat cgagcgcgac caacagcggc gcggctatct cacctgagga ggcgccatgg
     4741 cgatcgatct ctccgcgaag attccgaaca acgtcgacct cgacagcgat ccgaaactgc
     4801 ggcgcgcgct cgaagcgtgg caaccgcagt acgtcgagtg gtggaaggag gtcggcccgg
     4861 tcggcttcca gaacgcggat gtctatctgc gcacggcgat cgacgtcggc gcgcaaggct
     4921 gggcgaactt cggctacgtc aagatgccgg actatcgctg gggcatcttc ctcgccacgc
     4981 ccgagccgca ccgcacgatc ggctttggcg accacgccga cgcgccggtc tggcaggacg
     5041 tgcccggcga gcaccgcgcg gcgcttcgca ggctcgtcgt gacacaagcc gataccgaac
     5101 cggccagcgt cgaacagcag cggcgactcg gcgcgacctg cccgtctctc tacgacctga
     5161 ggaatctatt ccaggtcaat gtcgaggagg gccggcacct gtgggcgatg gtctatctgc
     5221 tacacaagta cttcggccgc gatggccgtg acgaaggcga cgagctgctc gcgcgccgcg
     5281 ccggctccgc agacaagccg cgcatcctcg agacgttcaa cgagcccgtc gaggactggc
     5341 tcgacttctt catgttcacg atgttcacgg atcgcgacgg taagtttcag ttgctgtcgc
     5401 tcgccgagag cgcgttcgat ccgctcgctc gcagctgccg gttcatgctg accgaggagg
     5461 cgcaccactt gttcgtcggc gagaccggca tcgatcggat cgtgcagcgc tcggccgaga
     5521 tgacgcgtta cgatcgcaac ggagatgcgt atcgccacgg cgcgatcgac ctgccgatca
     5581 tccagcgcta tctgaatctg tggttcagct cgtgcctcga cttgtttggc ggcgaggtgt
     5641 cgtcgaatgc ggcgaacaac ttcgccgctg gcctcaaggg gcgcgcacac gagagcaagt
     5701 atcacgacca cgacgaacgg ggcgatgtgc ggtttgtcga ggtcgtcgac gacgatcacg
     5761 tgatcactcg cgagatcgcc gcgcgcctcg cgatcaacga ggacacgcgc gacgcgtaca
     5821 tccgcgactg cgaacgcgcg gtcgcgcgct ggaaccgcac gatccgcaac tgcggcctcg
     5881 acttcgagct cacgctgccg catcgccggt tccatcgcgc catcggtgag cacgccggct
     5941 ggtgcttcgc acctgatggc aggttgatca gcgtcgagca gttcctcacg cacgaggacg
     6001 agtggctgcc gagcgccgcc gaccgtgcca aagtgcgtgc gctgcaaacg cgcccggtca
     6061 ctgtaccggg tcagttcgcg agctggatcg cgccgccgct gcgtggcgtc gacggcaggc
     6121 cgctcgactt cgagtacgtc aggctggact gatgacacgc cggtcgatca tcgagccgtt
     6181 caagatcaag gtcgtcgagc cgctgccaat gcgcacggtc gccgagcgcg aagcgatcct
     6241 cgccgcgtgc ggctacaacc tgttcggggt ccgcgcggcc gacgtcacgt tcgacttcct
     6301 caccgactcc gggaccaccg cgatgagcgc cgcccagtgg ggcgcgatga tgacggccga
     6361 cgagagctat gccggctcgc gcagcttcta tcgcttcgag gacgtcgttc gccacatcac
     6421 cggctacgac cacatcatcc cgacgcacca gggccgcgcc gcggagcacc tgctgttctc
     6481 gctgcactgc aaacgcggcg aggtcgtgcc gtcgaacaac cacttcgaca ccacgcgcgc
     6541 gaacttggag cagctcggcg tcgaagccgt cgacctcgtg tgtgccgaag cgcgcgaccc
     6601 ggcgagtgag gtgccattca agggcaacat cgacgtgccg aggctcgagg cgttcctgcg
     6661 cgagcaccgt gtccgcgtcc cgttcgtgat gaccacgatc acgaacaaca ccggcggcgg
     6721 acagccggtc tcactcgcga acctgcgcgc ggtgtccgcg gtgtgccggc gcgagggcat
     6781 cccattcctc ctcgacgcgt gccgattcgc cgagaacgcg tggttcatca aggtccgcga
     6841 gcctggccaa gccgaccgca cgccgcgcga gatcgcgcac gagatgtttg cgctcgctga
     6901 tggctgcctc gtcagcgcga agaaggacgg gctcgtcaac atcggcggct tcatcgcact
     6961 gcgcgacggc gcgtgggttc ccgacctgcg cagccgcctg atcctcaccg agggctttcc
     7021 gacgtacggc gggctcgccg cgcgcgatct cgaggccatc gcggtcgggc tcgacgaagt
     7081 gctcgacgag cgctacctcg cgtaccgcga agcggtcacg cgctacgtcg ccgacggcct
     7141 gcagcgcgcg ggcgtcccga cgatgcgccc acccggcggt catgcggtgt acctcgatgc
     7201 aaaggcactg ctgccgcatg ttccgccagc gcagtttcct gcccaggcgc tcgcggtcga
     7261 gctgttccgc gccgagggca tccgcagctg cgagatcggt tcggtgatgt ttggaaaact
     7321 tgccgcgctc gagctggtgc ggctcgcgat cccgcgacgc gtctacacgc aggcgcacat
     7381 ggactacgtg ctcgagggca tcgccgagat cgccgaacgg cgcgccacgc tacgcgggat
     7441 gcgaatcgtc gaggagccac cgttcttgcg ccacttcacg gcgcgattcg cgccgctgga
     7501 atagcagcgg cgatcatcgc gttgcagcga gatgaggacc cggacgcggg cgcagctggc
     7561 gacgatcgcc gcgggcgcga ccctgctcgt cgcgatcatc ggcgtgtcgc tcgtgcttcg
     7621 attgcagcaa cgcgcgcgcg agctcacgta catcccgccg ccgtgggatg tccctgcact
     7681 cgcaccggag cccgtggtgg gtccggcgt
//
LOCUS       DASUFX010000500         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1885004, whole genome shotgun sequence.
ACCESSION   DASUFX010000500 DASUFX010000000
VERSION     DASUFX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885004"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFV99_20040"
     CDS             <1..1173
                     /locus_tag="VFV99_20040"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEX5061673.1"
                     /translation="GLLTGAALLAVPRPASPTRAAIQIDCTVAQHCTFAESLALDVWS
                     EQRGPNLPLDIVVSSDSLARLDAAHVPWQMLDPDIDATARAEAVRLHGPAAAQPADFF
                     ADYRDYRAITERMTQLAAAAPDRVRMEGIGSTLDGRTIWALHIGPRNATPMLINGTQH
                     AREWIAAMTTTCVADRLVRDYDRDPAIKKFVDSTSLYVVPVVNPDGYQWSWSNNRYWR
                     KNRRGGFGVDLNRNFSVGWGGTGSSSRQGAEDYRGEAAFSENESAALRDLAKRERIAL
                     HVDFHSYGQLLLYPWNYSPQQAKDRARFAAIGDRMASAIYAQHEKSYKLMQGVELYPA
                     AGTLSDWAYGDANALSFTIELRPKMGRGGFVLPPSEIKPTCDEGVAAVLALRAAQP"
     gene            complement(1229..1984)
                     /locus_tag="VFV99_20045"
     CDS             complement(1229..1984)
                     /locus_tag="VFV99_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061674.1"
                     /translation="MLAKIHADEPRSARDLRGEVAAAQSALLLIVGIVASAPLALVAV
                     ALVHPQPPWRDAAQFAAEFHWIQVTPYVGGFLLVASCVALIASLCSLMPAARRARANF
                     ALAVTAAFAALIVLNYIVQTTLVPSLVSPYHDDNGPLVTMLTMSSPRSFGWALEMWGY
                     AVFGVATWAIAPAFGSSRLERATAALFVLNGIASIAAAVATSLWPGSPMTAPGFIGFA
                     VWNALMVTMAVLVILVMRRRVSGNVATPRARLG"
     gene            complement(2013..2702)
                     /locus_tag="VFV99_20050"
     CDS             complement(2013..2702)
                     /locus_tag="VFV99_20050"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5061675.1"
                     /translation="MSALVAQRWPELDADADIRAGHIWVDGFPRTNPAMLVARDASVV
                     LRTPQPLAGEAKLRAALAAFAPVVADRVAMDVGAAAGGFTRVLLEAGARRVYAVDAGF
                     GQLLGSLRQDARVVNLERTNLGALDRMLVPDVIELVTIDVSYISLARAVPQLAQLAFA
                     PDAELVALVKPMFELALAEAPEDPELLADALRRATAGMSDVGWDIVGAIDSPVRGHHG
                     AKEMLVHARRR"
     gene            2787..3836
                     /locus_tag="VFV99_20055"
     CDS             2787..3836
                     /locus_tag="VFV99_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5061676.1"
                     /translation="MRAWALCLALAACGDDAMTPTGDGSMPTPDSPPAVVEDIHFIGR
                     FDAMHRFAWPGSQIRTRFEGTTISIDLDDSGMSYYDVTIDGTTTTVHPASGRHMLALA
                     SGLPAGMHDLAFSRRTESFFGIATFYGFSGATLIPTARPQRLIEFIGDSITCGYGVLG
                     AVATCNFTADTEAETHAWGTFASTALDAVHVSIAYSGLGMYRNYGGDTSPTIPQRYGR
                     TFADDNASTWDFSYTPDVVVINLGTNDFSGGDPGAPFVTAYVGFVQMLRSKFGNAAIV
                     LAASPMLGAPDHAKHKAYLDQVASMVADANVSVVDIATQLASDGYGCDYHPNEVTQHK
                     MSDQLVPAIRAATGW"
     gene            complement(3837..4697)
                     /locus_tag="VFV99_20060"
     CDS             complement(3837..4697)
                     /locus_tag="VFV99_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061677.1"
                     /translation="MRYAALLLLVGCLDLGDSEDEDLVDTTSDALSLAPSPGENGSYC
                     IASPYNCRFRDGDSRVLNAGGDDVWAVMPGASVRDGDGNVLAMQTGDRMTFNYGQTRA
                     LAGKAHALALTTTNSSAGWYPFDHIVSEASFRDHVGNVDAKSPHAGHMACYEIRNSHD
                     ASIELKKVVYDSKVGPNGHERAGDYLSLVRANGRRSANLIFNVPGFGLGGAADDHFPA
                     GTKFHRVTVPTKSGVPSISIPLWVADSNGRYVVRSGSLRFFYGYIVGEGVSRFGWMAQ
                     DALQVSTGCP"
     gene            complement(4818..4958)
                     /locus_tag="VFV99_20065"
     CDS             complement(4818..4958)
                     /locus_tag="VFV99_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061678.1"
                     /translation="MATHVQTHPDHRNEETWGGPFFSIATPIIAAVLSLLLAIVVVLT
                     AK"
     gene            5005..>5258
                     /locus_tag="VFV99_20070"
     CDS             5005..>5258
                     /locus_tag="VFV99_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061679.1"
                     /translation="MWNLGTAWQDELALAAAWRAGQPFPHVIADDFVPRDALPELFAV
                     LDDEAVDRYEGDIFRFEASAVEPATPEFRGLRDEFAAVLAP"
BASE COUNT          901 a         1762 c         1761 g          834 t
ORIGIN      
        1 ggcctcctga ccggcgcggc attgcttgcc gtgccccgac ctgcgtcgcc gacgcgcgcg
       61 gcgatccaga tcgactgcac ggttgcgcag cactgcacgt ttgccgagtc actcgcgctc
      121 gacgtgtgga gcgagcaacg cggcccgaat ctgccgctcg acattgtcgt gtcatccgat
      181 tcgctggcgc gtctcgacgc cgcgcatgtg ccgtggcaga tgctcgatcc ggacatcgac
      241 gcgactgcac gtgccgaagc cgtgcgcttg cacggtccgg cagcggcgca gcctgccgac
      301 ttcttcgcgg actaccgcga ctaccgcgcg atcaccgagc gtatgaccca gctcgccgcg
      361 gcggcgcccg atcgcgttcg catggagggc atcggcagca cgctcgacgg ccgcacgatc
      421 tgggcgctgc acatcggtcc gcgcaacgcg acgccgatgc tgatcaacgg cacgcagcac
      481 gcgcgcgagt ggatcgccgc gatgacgacg acgtgtgtcg ctgatcgtct cgtgcgcgac
      541 tacgatcgcg atccggcgat caagaagttc gtcgacagca cgtcgctgta cgtcgtgccg
      601 gtcgtcaatc ccgacggcta ccagtggtcg tggagcaaca atcgctactg gcgcaagaac
      661 cggcgcggcg ggttcggcgt cgatctcaac cgcaacttct ccgtcgggtg gggcggcacc
      721 ggttcgagca gccggcaggg cgccgaggat tatcgcggcg aggcggcatt ctcggagaac
      781 gagagtgctg cgctacgcga cctcgcgaag cgcgagcgaa tcgcgctgca cgtcgacttc
      841 cattcctatg gtcagctgct gctctatccg tggaactact cgccgcagca ggcgaaggat
      901 cgtgctcggt ttgcggcgat cggcgatcgc atggcgtcgg cgatctacgc gcagcacgaa
      961 aaaagctaca agctcatgca aggcgtcgag ctctatcccg cggccggcac gctctcggac
     1021 tgggcgtacg gcgacgcgaa tgcgctgtcg ttcacgatcg agctgcgacc gaagatgggt
     1081 cgtggcggtt tcgtgctgcc gcccagtgag atcaagccga cgtgcgacga aggcgtcgcg
     1141 gcggtgctcg cattacgcgc tgcgcagccc tgaagctgcg tagactcgtg tgcacatcac
     1201 cgcgctgagc acgaggtgtc agcactgctc agccgagccg cgcgcgcggc gttgcgacgt
     1261 ttccactgac acggcgccgc atcacgagaa tcacgagtac cgccatcgtg accatcagcg
     1321 cgttccacac tgcgaatccg atgaacccgg gcgcggtcat cggcgagccc ggccagaggg
     1381 acgtcgcgac ggcagcggcg atgctggcga taccgttgag cacgaatagc gccgcggtcg
     1441 cgcgctcgag acgcgagctg ccgaatgccg gcgcgatcgc ccacgtggcg acgccgaaca
     1501 cggcatagcc ccacatctcg agcgcccacc cgaacgaccg cgggctcgac atcgtgagca
     1561 tggtgacgag cggcccgttg tcgtcgtggt acggggagac cagcgacggc acgagcgtcg
     1621 tctgcacgat gtagttgagc acgatcagtg cggcgaatgc cgccgtcact gcgagcgcga
     1681 agttcgcgcg ggcgcgccgt gctgcgggca tcaacgagca caggctcgcg atcagcgcga
     1741 cgcagctggc gaccagcagg aagccaccga catacggcgt gacctggatc caatgaaact
     1801 cggcggcgaa ctgcgcggcg tctcgccatg gcggctgcgg gtgcacgagc gcgacggcca
     1861 ctagcgcaag tggggccgat gccacgatgc ccacgatcaa gagcagcgcc gattgcgcgg
     1921 ccgctacttc accgcgcaag tctcgcgcgg atcgcggctc gtcagcgtgg atcttggcca
     1981 gcatggccgg gcgagatgcg taaaccgtgc cgtcagcgcc tgcgcgcgtg gaccaacatc
     2041 tccttcgcgc cgtgatgacc gcgcaccggt gagtcgatcg cgccgacgat gtcccagccg
     2101 acgtcgctca tgccggcggt tgcgcggcgc agcgcatcgg cgagcagctc cgggtcctcg
     2161 ggcgcctcgg cgagcgcgag ctcgaacatc ggcttcacga gcgcgacgag ctcggcatcc
     2221 ggcgcaaacg cgagctgcgc gagctggggg accgcgcgcg cgagcgagat gtacgagacg
     2281 tcgatcgtca cgagctcgat cacgtcgggc acgagcatgc gatcgagtgc gccgaggttc
     2341 gtgcgctcga ggttcacgac ccgcgcgtcc tggcgcaacg agccgagcag ctgaccgaag
     2401 ccagcgtcga ccgcgtagac gcgacgcgca ccggcctcga gcagcacgcg cgtgaagcca
     2461 cccgccgcgg caccgacatc catcgcgacg cgatcggcga cgaccggcgc gaacgcggca
     2521 agcgccgcac gcagcttcgc ctcaccggcg agcggctgcg gtgtgcgcag cacgacgctc
     2581 gcgtcccgag cgacgagcat cgccgggttc gtgcgcggga agccatcgac ccagatgtgg
     2641 ccggcgcgga tgtcggcgtc ggcatcgagc tcgggccacc gctgggcgac gagcgcactc
     2701 aatttctaac gtcaaccctt ggccatgatg cggtgccaac cgaggtcggg gccctcgcat
     2761 tcccgcgatc gtgagaagat gacggcatgc gggcttgggc gctttgtctg gcgctggcgg
     2821 cgtgtggcga cgacgccatg acgcccacgg gcgacggcag catgccgact cccgacagcc
     2881 ctcccgccgt tgtcgaagac atccacttca tcggccggtt cgacgcgatg caccgcttcg
     2941 cgtggccggg ctcgcagatc cgcacgcggt tcgaaggcac gacgatcagc atcgatctgg
     3001 acgacagcgg gatgagctac tacgacgtca cgatcgacgg tacgacgacg accgtgcacc
     3061 cggcgagcgg ccgccacatg ctcgcgctcg catcgggcct ccccgccggc atgcacgatc
     3121 tcgcgttctc gcgccgcacc gagtcgttct tcggcatcgc gacgttctac ggcttctcgg
     3181 gcgcgacgct gatcccgaca gcgaggccgc aacgcctcat cgaattcatc ggcgattcga
     3241 tcacgtgcgg ctacggtgtg ctcggcgcgg tggcgacgtg caacttcacc gcggacaccg
     3301 aggcagagac gcacgcgtgg ggaacgttcg cgtcgacggc actcgatgca gtgcacgtct
     3361 cgatcgcgta ctcggggctc ggcatgtatc gcaactacgg aggcgacacg agtccgacga
     3421 ttccacaacg ctacggccgc acgttcgcgg atgacaatgc gagcacgtgg gacttcagct
     3481 acacgcccga tgtcgtcgtg atcaacctcg gcacgaacga cttttccggc ggcgatccgg
     3541 gcgcgccgtt cgtcaccgcg tacgtcggtt tcgtccagat gctgcgcagc aagttcggca
     3601 acgcggcgat cgtccttgca gcgagcccga tgctcggtgc gcccgatcac gcgaagcaca
     3661 aggcgtacct cgatcaagtt gcgtcgatgg tcgcagacgc caacgtctcg gtcgtcgaca
     3721 tcgcgacgca gctcgcgagc gacggctacg gctgcgacta ccacccgaac gaggtcacgc
     3781 agcacaagat gagcgatcag ctcgtgcccg ctattcgcgc cgccaccggt tggtgatcac
     3841 ggacagccgg tcgagacctg cagcgcgtcc tgggccatcc agccgaaccg gctgacaccc
     3901 tcgccgacga tgtagccata gaagaagcgc aacgagcccg accgcacgac gtagcgaccg
     3961 ttgctatccg cgacccacag cgggatcgag atcgagggca caccgctctt cgtcggtacc
     4021 gtcacgcgat ggaacttcgt gccggcaggg aagtgatcgt ccgctgcgcc gccgagtccg
     4081 aagccgggca cgttgaagat gaggttcgca ctgcgccgac cgttcgcgcg cacgagcgag
     4141 agatagtcgc ccgcgcgctc gtggccgttg gggccaacct tgctgtcgta gacgaccttc
     4201 ttcagctcga tgctcgcgtc gtgcgaattg cggatctcgt agcaggccat gtggccagcg
     4261 tgcgggctct tcgcatcgac gttgccgacg tgatcgcgaa acgatgcttc gctgacgatg
     4321 tgatcgaacg gataccagcc cgcgctgcta ttggtcgtcg tcagcgcaag cgcgtgtgcc
     4381 ttgccggcga gcgcacgcgt ctgcccgtag ttgaacgtca tgcgatcgcc ggtctgcatc
     4441 gcgagaacgt tgccgtcgcc gtcgcgcacc gacgctccgg gcatgacggc ccacacgtcg
     4501 tcacctcccg cgttcagcac ccgcgagtcg ccgtcccgga agcggcagtt gtacgggctc
     4561 gcaatgcagt agctgccgtt ctcgcccggt gagggagcga ggctcagcgc atcactcgtg
     4621 gtgtcgacga gatcctcgtc ttcgctgtcg ccgagatcga ggcaaccgac gagcagcagc
     4681 agtgcggcat atctcatggc gccgcgtatg ctgcagtcgc catgcctcgt gtggttcgtc
     4741 gacctgaatg tgcttttggc ggcaacggcg cgatgccgcg acgcgctgtg cacacggttg
     4801 tcagcagacg gtgcccgtca ctttgcagtg aggacgacca cgatcgccaa gagcagcgag
     4861 agcaccgcgg cgatgatcgg ggtcgcgatg ctgaagaacg ggccacccca ggtttcctca
     4921 ttgcggtgat cgggatgggt ttggacgtgc gttgccatac cggcaggtac tgcatcgcct
     4981 gggccgacag ggtacgaatg cgagatgtgg aatctcggca ctgcgtggca ggacgagctg
     5041 gcgctcgccg ctgcatggcg cgcggggcag ccgtttccgc acgtcatcgc cgatgacttc
     5101 gttccgcgcg acgcgctgcc cgagctattc gcagtgctcg acgacgaggc agtcgatcgt
     5161 tacgagggcg atatcttccg gttcgaggcc tcggcagtcg agccggcgac gcccgagttt
     5221 cgcgggctgc gcgacgaatt cgcagcggtg ctcgcccc
//
LOCUS       DASUFX010000501        12887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1330047, whole genome shotgun sequence.
ACCESSION   DASUFX010000501 DASUFX010000000
VERSION     DASUFX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330047"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..668)
                     /locus_tag="VFV99_20075"
     CDS             complement(30..668)
                     /locus_tag="VFV99_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061680.1"
                     /translation="MTEIEPPSDDLRALFASERDISAVERAAIRTKLGATLATPAAAT
                     TAVATTKLIWIAAAALATAAGIWWLVHRAEPAAVPRAPTIETGSAITPEPSIAITAPE
                     PSVAAPKPSVAVAEQQAPQAAAPIPSQAELLAKAWQVLPHDPAEALKLVELDARLHAD
                     GALTEEREALRIQALLPLGRAADARELAKTFNERYPQSVHRKRLDAALGGHK"
     gene            complement(665..1159)
                     /locus_tag="VFV99_20080"
     CDS             complement(665..1159)
                     /locus_tag="VFV99_20080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061681.1"
                     /translation="MAESVPEVAEGIYAVYERELSYVWKTLGRLGVPAADLADAAHDV
                     FVIVHRRWADIDFERPIRPWLFGVARRVAAGMRRKHRELPSQHLQSVVDASPHAERDL
                     LWRVLASLDDDRRAVVILHDLEGYTGAEIGEILGISANTVHSRLRLAREDMRATLSKL
                     RGAP"
     gene            complement(1192..2112)
                     /locus_tag="VFV99_20085"
     CDS             complement(1192..2112)
                     /locus_tag="VFV99_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061682.1"
                     /translation="MRLIAIAAMLCVAHTSAAAPCTREAVAGEVRALGGDLDGAGDRV
                     QIVMHRDGRGAEGSIVYTSAAGDVTAPRVVHATSCGELAKSLALIVVMSLRADEQVDA
                     PAPVMAPAMIPPATTPPMTTTPTTTMTSNELVNHSTTSDTRAHSSTRHVALFVGAASD
                     TTAHPALELGSRWRHDRLSLGLELRIATPESIDVGDGGTVNVATAGLDAVPCFHAGYL
                     GLCGLATAGMISAEGQALANAATARRAAFAIGGRIELDVPLLHRLSLRVHLDGLQALT
                     SSRFLVDQMPVWTSEPRELWLGAGVLAKFP"
     gene            2247..3233
                     /locus_tag="VFV99_20090"
     CDS             2247..3233
                     /locus_tag="VFV99_20090"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5061683.1"
                     /translation="MRHLTIIAILVGTARADTQVSVRWGTYTERERTALVRLHGAEDA
                     TIRGATIELASSETAVTAKLRFNIATKSREALTIRVPIELTSGTAVTGFRYGIGGEPT
                     IEAITRSADDALMTFNRIVERQKDPALLRLVKRKEKRDMLELAVYPVTRGMPASVEID
                     LTLPQATRLVLDSGPRQTEQAFALPETRREWAMSDPASHEQLDAATSWFAGDVRWSPA
                     RSAKPDRRVRMTPTVALSDRRYDVRVAIREHATQLAHCYAAGVARDATLSPSAMLAID
                     IATDGRVSHAEVTDYADADVRWCIEDEIAGWRFTAADNARRVRQDVDLTNLE"
     gene            complement(3234..4157)
                     /locus_tag="VFV99_20095"
     CDS             complement(3234..4157)
                     /locus_tag="VFV99_20095"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEX5061684.1"
                     /translation="MMNRTLFTSTLLLCAALVACGDQDEPLSAGEQAVGNGNAYGHYK
                     RCSTRTPSDTEIAKADEVVKNAQGKPGGGGGHGGGGGGGGGTTVTGGTVAVHFHVITD
                     GTTGNLSETDINNQITVLNDAYAPTGWQFQLLTVDFTTNASWFDTCDTSSTENAMKSA
                     LRTGTAADLNIYTCNPGGGLLGWATFPSSYASNPKDDGVVILYSSLPGGSAAPYNEGD
                     TATHEVGHWMGLYHTFQGGCSKTGDSVSDTPAERSAQFGCPVGANTCNGTGLDPIENF
                     MDYTDDSCMFEFTAGQDARMDALFSAYRYGK"
     gene            4368..8012
                     /locus_tag="VFV99_20100"
     CDS             4368..8012
                     /locus_tag="VFV99_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX5061685.1"
                     /translation="MDKPVPDKVVIGIDVGSTTVKMTVVDPTTKEILWSKYLRHETRQ
                     PEMTREMLREIHAAFPTLKNDQIRTFITGSGGSPIAPHLGSKFVQEVNAVTMAVEKLH
                     PDVGSVIELGGQDAKIIMFKENKDTGDKTAQTSMNDKCASGTGATIDKCVLKVGMPRE
                     EVPGLMFDPTKLHHVAAKCGVFAETDIVNLVKSGIPRNEIMNSLADAIVSQNLAVLTR
                     GNTLKAKVLLLGGPNTYLPFLQQCWRLRIPEAWAERGYEYDKTVPIEQLIFVPKNSDL
                     YAAYGAVLFGLYEPEAIGAYRGLEPLDEFINHGRKAKLGESAGPGLVEVKDEAVAFSE
                     KYKTPDYEDAKLEAGKTYRGVIGLDGGSTSSKCVFIDENENILKKVYTLSKGNPIQDM
                     KDMFADMRKWATDQGATLDVSGFGVTGYAGDVLERSLGADANIVETVAHMMSAKRWFP
                     AVDVICDIGGQDIKVMFMQNGDVKNFRLSNSCSAGNGMLLQAMADQFGVPVKQYAEVA
                     FEARLAPKFSYGCAVFLDSDRVNFQKEGYAKEELLAGLALVLPKNIWQYVVQIPRMSE
                     LGRVFVLQGGTQKNLAALKAQVDYIEKRVPNAEVYLHPHTGEAGAIGAAFEALRVTRR
                     RGKTTFVGLEQAIDIEYTSTNDDKTRCNFCPNNCSRTFIDTKTLDGKTARYISGFSCE
                     KGTVENMDALKVLNKERQKRMKQYPNLVDYEAKLAFKSFYEHAAMPEHGTPVKDVVVK
                     RTLLGAVRHKPIERPFQRASKEVWEKRRDVRVGIPRVLNLYSTGPFWRTYFETIGLDS
                     RNVVFSDETSEEMWQAGCKYGSVDPCYPSKVCQAHIHNLLFKHHEKKPLNYIFFPAIT
                     HIPTYIVNQMDTASCPVVSGTPKVIRAAFTKETDFFAERGISYLDTAVTLNEALMCKK
                     QMFNEWGPLLGLTEDESDFAIDEAFKAVQAFEDEMERKGKEILDEVVKDNRVALVMLG
                     RPYHNDPGLNHSVLEEFQALGYPILSMRSLPRDKPTLNWLFAEDLGKKVIESPMDVTD
                     VWPENYSTNSVQKVWAAKYAARHPNLACLDLSSFKCGHDAPTYGLIDSVIASAGKAYS
                     ALHDIDANKPSGSIKIRVKTYAHTLMLLREKLEDQATKKTELDRTLSAKKLELMKSLQ
                     AKLAGVGRTDDKLDVEVKALETQVAAWAAEEAAKNPKAKKLASLNVIKDGSGAGSSAA
                     ANLAKAKEEQ"
     gene            8009..9880
                     /locus_tag="VFV99_20105"
     CDS             8009..9880
                     /locus_tag="VFV99_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HEX5061686.1"
                     /translation="MSTTNNKSGENLDPMSLEAIEEQLKQFEIDERKRLGLPVEATKH
                     WLDEVPRQFTRDQREHTTILVSGLTMAHDLFIQAALRGIGYKVLAMDVPDNDALQYGR
                     EYGNRGQCNPTYFTVGNLVKYLDGLRDRGMSKDDIVKNHLFITAGACGPCRFGTYVTE
                     YRKALRDSGFDGFRVLLFQQTGGLKQATGEGVGLEMNPTFFWGVVRAIMIGDALNALG
                     YRIRPYEVEAGATDRAIESSKKIISDAFENNTSLPMALWRVRRTMSKVKVDRTKPKPR
                     VGIIGEFWAMTTEGDGNYGLQRFLEAEGAEPEIQLVTAWLLYMIWQGRYDTAQRASLR
                     GADKAFKDADGKQQVGGSKFSLAGVDVRKRIASLWAGEQVLRGLFQTFAHAMGLYDYH
                     LPDMNQIAEISHAFYDNNLRGGEGHMEVGKLIQNVAYQKVNMTLSVKPFGCMPSSSVS
                     DGIQSFITELYPQGIFLPIETNGDGAVNVYSRVQMQLFKAKQLAQKEVDKALADVGMT
                     IEEVRTYLDKHPSLAHAFHRAPHKAGCTAADLIYEVGARKNRLRYAKDSVVAKLTGKA
                     KKEHHDAHVQLLAIARKAAEKTMRKKSVVTVAPELDPNDDSGRPAVPAGRKSLRVVN"
     gene            10651..10860
                     /locus_tag="VFV99_20110"
     CDS             10651..10860
                     /locus_tag="VFV99_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061687.1"
                     /translation="MIPDGTCITVNSFTACGWATECDPSTPQTCIGTTLQSCVLGALK
                     TVDCTTIGGTQCVPNTQAPGAWCSR"
     gene            10899..11678
                     /locus_tag="VFV99_20115"
     CDS             10899..11678
                     /locus_tag="VFV99_20115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061688.1"
                     /translation="MEPRDFSTIGPSARMLLVMRAQSDLPFARQSAELLLGAERVAQD
                     IARLAELAGSALRLRHFVARYRSIDTLLAESGAIRVLELGGGLSLRGLAWAERNAGTY
                     LDTDLPDMVRTKQQLMDALAVGPLVGELRLAALNALDADAFRASVATLPAGPIAIVNE
                     GLLMYLDDAEKQQLATNIRDALSARGGVWITADIYVQGERSARIERSEREREFLAAHR
                     VEENKFASLTAAESFFTGAGFTIVRRLPSDVTRESWVVEAR"
     gene            11697..12164
                     /locus_tag="VFV99_20120"
     CDS             11697..12164
                     /locus_tag="VFV99_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061689.1"
                     /translation="MSLTDRLRARAHAALERVPGKVGDKLRGANEALGRPFADATELA
                     DRRAFDTRGGVHQSAAPAAAKAQREQAPVIVYYMEKQKRDVSKLTDVLDANGIAYTAT
                     NIQEDPAAQFAVRRDSKGIRLPVVFVAGDCIGGRIELINAASSGELKKKVFGS"
     gene            12208..>12887
                     /locus_tag="VFV99_20125"
     CDS             12208..>12887
                     /locus_tag="VFV99_20125"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5061690.1"
                     /translation="MIGTQERKAGQAESAAPSAENEAPNAAPSHQRFTNEGPIPPVAQ
                     SSNAVAPGASDLAFGAHDEHYATADNSLDDANHLRVKATGLNVRSSPNARGHANIVGL
                     LHHGDQIERVGKAGHWVQISYQGQQAFVHGAYVEAAHHAEAKPAKAPSEPQPESVANA
                     VEKATQHAPEIKQHLPEKKVTENAPHASATHAPSETKAPESKPAAENPASTAPVINHA
                     PVSNAPVA"
BASE COUNT         2440 a         4165 c         4234 g         2048 t
ORIGIN      
        1 cgcaatgaac gcgaggaccg cgagccgctt cacttgtgac ctccgagcgc ggcatcgagg
       61 cgtttgcggt gcacgctttg cggatagcgc tcgttgaacg tcttcgcgag ctcgcgtgca
      121 tctgcggcac gaccgagtgg gaggagcgcc tggatgcgca gcgcttcacg ctcttcggtg
      181 agcgcgccgt ctgcatgtag acgcgcgtcg agctcgacga gcttcagcgc ttccgcagga
      241 tcgtggggca gcacctgcca ggccttcgcg agcagctcgg cttgcgacgg aatcggtgcg
      301 gctgcctgtg gcgcttgttg ctccgccacg gcgacgctcg gctttggtgc ggcgacgctc
      361 ggttccggtg cggtgatcgc gatcgatggc tcgggcgtga tcgcggagcc ggtctcgatc
      421 gtcggcgcac gcggaaccgc agccggctcg gcgcgatgca cgagccacca gatgcccgcc
      481 gcggttgcca gcgcagcggc cgcaatccag atcagcttcg tggtcgcgac ggcggtcgtc
      541 gctgccgcgg gcgtcgcgag cgtcgcgccg agcttcgtgc ggatcgccgc gcgctcgacg
      601 gcgctgatat cgcgctcgct cgcgaacagc gcgcgtagat cgtcgctcgg tggctcgatc
      661 tcagtcatgg cgcgcctcgc agtttggaca gcgttgcgcg catgtcctcg cgcgcgagcc
      721 gcagtcggga atggacggtg ttcgccgaga tgccgaggat ctcgccgatc tccgcgccgg
      781 tgtagccctc gaggtcatgg aggatgacaa ctgcgcgccg gtcgtcatcg aggctggcga
      841 gcacgcgcca caacagatcg cgctcggcat gtggcgacgc atcgacgacc gactgtaaat
      901 gctgactcgg cagctcgcgg tgcttgcgcc gcatgccggc ggcaacgcgg cgcgcgacgc
      961 cgaacagcca aggccggatc ggtcgctcga agtcgatgtc ggcccagcgc cgatgcacga
     1021 tgacgaacac gtcgtgcgca gcgtcggcga gatccgccgc cggcacgccg aggcggccga
     1081 gggtcttcca gacgtagctg agctcgcgtt cgtaaaccgc ataaatgccc tccgcgacct
     1141 ccgggacgga ctcggccacg aagaggtatt gccggagggc atccgttttc gtcacgggaa
     1201 cttcgctagc accccagccc cgagccacag ctcgcggggt tcgctagtcc atacgggcat
     1261 ttggtcgaca agaaaacgtg atgatgtcag cgcctggagc ccatcgaggt ggacccggag
     1321 cgatagccgg tgaaggagcg gcacgtcgag ctcgatgcgg ccgccgatcg cgaatgctgc
     1381 gcggcgggcg gtggccgcgt tcgcgagggc ctggccttcg gcggagatca tgcccgcggt
     1441 cgcgaggccg cacagaccga gatacccggc gtggaagcac ggcaccgcgt cgaggccagc
     1501 cgtcgcgacg ttcaccgtgc cgccgtcgcc gacatcgatc gactcgggcg tcgcgatacg
     1561 cagctcgaga ccgagcgaga gccggtcgtg ccgccagcgg ctaccgagct cgagggcagg
     1621 gtgggcggtc gtgtcgctcg ctgcaccgac gaagagcgcg acgtgccgcg tcgatgaatg
     1681 cgcacgcgtg tcgctcgtag tcgagtggtt gacgagctcg ttcgacgtca tcgtcgtcgt
     1741 cggtgtcgtc gtcatcggcg gcgttgtcgc cggcggaatc atcgccggcg ccatcaccgg
     1801 cgcaggtgcg tcgacctgct cgtcagcgcg tagcgacatc acgacgatca gcgcgagcga
     1861 tttcgcgagc tctccgcacg acgtggcatg tacgacccgc ggggctgtca cgtcaccggc
     1921 ggcgctcgtg tagacgatgc tgccttccgc gccgcggccg tcgcgatgca tgacgatctg
     1981 cacgcgatcg ccggccccgt cgagatcgcc gcccagcgcg cggacctcgc cggcgaccgc
     2041 ctcgcgggtg cacggtgcgg cggcgctggt gtgcgccacg cagagcatcg ccgcgatcgc
     2101 gatcagcctc acgcactcac cttaccggcc gcgcggccgc gttgtcagcg aacaagttgt
     2161 gcaacggtgc tgacattgcg acttctttca caagatcgtg acgtgatcga cggaaatgct
     2221 gacacggatg tcgctgtcgc ggttgcatga ggcacctcac gatcatcgcg atcctcgtcg
     2281 gaaccgcccg cgcagatacc caggtcagtg tccgttgggg cacgtacacc gagcgcgagc
     2341 ggaccgcgct cgtacgtctt cacggcgccg aagacgcaac cattcgcggc gccacgatcg
     2401 agctcgcgag ctcggaaacc gccgtgaccg cgaagctgcg gttcaatatc gccacgaagt
     2461 ctcgcgaagc tctcacgatc cgcgttccga tcgagctgac gtcgggcacg gcggtgaccg
     2521 gctttcgcta tggcatcggc ggcgagccaa cgatcgaggc gatcacgcgc agcgcggacg
     2581 atgcgctcat gacgttcaac cgaatcgtcg agcgccagaa agatccggcg ctgctgcgcc
     2641 tcgtgaagcg caaggagaaa cgcgacatgc tcgagctcgc ggtgtacccg gtgacgcgcg
     2701 gtatgccggc gtccgtcgag atcgacctga cgctgccgca ggccacacgg ctcgtcctcg
     2761 attccggacc gcgccagacc gaacaagcgt tcgcactgcc ggagacgcgt cgagagtggg
     2821 cgatgagcga tccggcatcg cacgaacagc tcgacgccgc gacgtcgtgg tttgccggcg
     2881 acgtacggtg gtcgcccgcg cgatccgcga agccggatcg ccgggttcgc atgacgccga
     2941 cggtcgcgct ctcggatcgg cggtacgacg ttcgcgtcgc gatccgcgag cacgccacgc
     3001 agctcgcgca ttgctacgca gctggggtcg cacgcgatgc gacgctgtca ccgagcgcga
     3061 tgctcgcgat cgacatcgct acagacggtc gcgtctcgca cgcagaagtc accgactatg
     3121 cggatgccga cgtccgctgg tgcatcgagg acgagatcgc cggctggcgc ttcacggcgg
     3181 ccgataacgc gcgccgcgtg cgccaggacg tcgatctcac gaacctcgag tgactacttg
     3241 ccgtagcgat acgccgagaa cagcgcgtcc atacgcgcat cctgaccggc cgtgaactcg
     3301 aacatgcagg agtcgtcggt gtagtccatg aagttctcga tcggatcgag accagtgccg
     3361 ttgcacgtgt tcgcaccgac cgggcaaccg aactgcgccg accgctccgc gggcgtatcg
     3421 gagaccgaat cgcccgtctt cgagcagccg ccctggaacg tgtgatagag gcccatccag
     3481 tggccgacct cgtgcgtcgc cgtgtcgccc tcgttgtagg gcgcagccga accacccggc
     3541 agcgacgagt agaggatcac gacgccgtcg tccttcgggt tcgacgcgta ggagctcggg
     3601 aacgtcgccc aaccgagcag accgccacct gggttgcacg tgtagatgtt tagatccgcc
     3661 gccgtgccgg tgcgcagcgc ggacttcatc gcgttctcgg tcgacgaggt atcgcacgtg
     3721 tcgaaccacg acgcgttggt cgtgaagtcg accgtcagca actggaactg ccaacccgtc
     3781 ggcgcgtacg cgtcgttgag gacagtgatc tggttgttga tgtccgtttc cgacaagttg
     3841 ccggtcgtgc cgtcggtgat gacgtggaag tgcaccgcga cggttccgcc ggtcaccgtc
     3901 gtgccgccgc cgccgccgcc accgccaccg tgaccgccac cgcctccggg cttgccctgc
     3961 gcgttcttga cgacctcgtc tgctttcgca atttcggtgt cactcggcgt acgggtcgag
     4021 caccgcttgt agtgcccata cgcgttgccg ttaccaaccg cctgctcgcc ggctgaaagt
     4081 ggttcgtcct gatcgccgca cgcgacgagc gccgcacaga gaaggagagt cgaggtgaat
     4141 agcgtccggt tcatcatcag ggcgcgagaa tccttgatgt acgacatcaa ctcaagctgt
     4201 tccttatgta ggcaagctca tagtccgctg cgtaacgcgg tgcgcactgc gcaccggatt
     4261 acccggaaag agatctgttc ttgcctgggt ttagaactgg actgacacgt cagtccagtc
     4321 atagactgac gcgtcagtct agctatgcgc aagtactgcg aggacccatg gacaagcccg
     4381 ttccagacaa agtcgtcatc ggcatcgatg tcggctccac gaccgtgaag atgaccgtcg
     4441 tcgatccgac gacgaaggaa atcttgtgga gcaagtatct gcgccacgag acgcggcagc
     4501 cggagatgac gcgtgaaatg ctgcgcgaga tccacgccgc gttcccgacg ctgaagaacg
     4561 accagatccg gacgttcatc accggctcgg gcggctcgcc gatcgcgccg cacctcggca
     4621 gcaagttcgt gcaggaagtg aatgccgtaa cgatggcggt ggagaagctc catcccgacg
     4681 tcggcagcgt gatcgagctt ggcggtcagg acgccaagat catcatgttc aaagagaaca
     4741 aagacaccgg tgacaagacc gcgcagacgt cgatgaacga caagtgcgcg tcgggcaccg
     4801 gcgcgacgat cgacaagtgc gtactcaagg tcggcatgcc tcgcgaagag gtgccgggcc
     4861 tgatgttcga cccgacgaag ctgcaccacg tcgcagcgaa gtgcggcgta ttcgccgaga
     4921 ccgacatcgt gaacctcgtg aagagcggca tcccgcgcaa cgagatcatg aactcgctcg
     4981 ccgacgcgat cgtcagccag aacctcgccg tgctcacgcg cggcaacacg ctcaaggcga
     5041 aggtgctgct gctcggcggc ccgaacacgt acctgccgtt cctccagcag tgctggcgcc
     5101 tgcgtatccc cgaggcatgg gcggagcgcg gctacgagta cgacaagacg gtgccgatcg
     5161 agcagctgat cttcgtgccg aagaacagcg atctctacgc ggcgtatggc gcagtcctgt
     5221 tcggtctcta cgagcccgag gcaatcggcg cgtaccgcgg cctcgagccg ctcgacgagt
     5281 tcatcaacca cggtcgcaag gcgaagctcg gtgagagcgc tggtcccggc ctcgtcgagg
     5341 tgaaggacga ggcggtcgcg ttcagcgaga agtacaaaac gcccgactac gaggacgcga
     5401 agctcgaagc gggcaagacg taccgcggcg tgatcggcct cgacggcggc tcgacgtcgt
     5461 cgaagtgcgt gttcatcgac gagaacgaga acatcctgaa gaaggtctac acgctctcga
     5521 agggcaaccc gatccaggac atgaaggaca tgttcgcgga catgcggaag tgggcgaccg
     5581 atcagggcgc cacgctcgac gtgtccggct tcggcgtgac cggttacgct ggtgacgtgc
     5641 tcgagcgctc gctcggcgcc gacgcgaaca tcgtcgagac cgtcgctcac atgatgagcg
     5701 cgaagcgctg gtttccggcg gtcgacgtca tctgtgacat cggcgggcag gacatcaagg
     5761 tgatgttcat gcagaacggc gacgtcaaga acttccggct ctcgaacagc tgctccgccg
     5821 gcaacggcat gctgctgcag gcgatggccg atcagttcgg cgtccccgtg aagcagtacg
     5881 ccgaggtcgc gttcgaagcg cgcctcgcgc cgaagttcag ctacggctgc gccgtgttcc
     5941 tcgactccga ccgcgtgaac ttccagaagg aaggctacgc gaaggaggag ctgctcgccg
     6001 gtctcgcgct cgtgctgccg aagaacatct ggcagtacgt cgtccagatt ccgcgcatga
     6061 gcgagctcgg ccgcgtgttc gtcctccagg gcggcacgca gaagaacctc gccgcgctca
     6121 aggcgcaggt cgattacatc gagaagcgcg tgccgaacgc cgaggtctac ctgcacccgc
     6181 acaccggtga agcaggcgcg atcggcgcgg cgttcgaagc gctgcgcgtg acgcgtcgcc
     6241 gcggcaagac gacgttcgtc ggtctcgagc aggcgatcga catcgagtac acgtcgacga
     6301 acgacgacaa gacgcgctgc aacttctgcc cgaacaactg ctcgcgcacg ttcatcgaca
     6361 ccaagaccct cgacggcaag acggctcgct acatctcggg cttctcgtgc gagaagggca
     6421 ccgtcgagaa catggacgcg ctcaaggtgc tcaacaaaga gcgccagaag cgcatgaagc
     6481 agtacccgaa cctcgtcgac tacgaggcga agctcgcgtt caagagcttc tacgagcacg
     6541 cggcgatgcc agagcacggc acgccggtga aggacgtcgt cgtcaagcgc acgctactcg
     6601 gcgcggtccg ccacaagccg atcgagcgtc cgttccagcg cgcgagcaag gaagtctggg
     6661 agaagcgccg cgatgttcgc gtcggcatcc cgcgcgtgct caacctctac tcgacgggcc
     6721 cgttctggcg cacgtacttc gagacgatcg gcctcgactc gcgcaacgtc gtgttcagcg
     6781 acgagacgag cgaagagatg tggcaggccg gctgcaagta tggctcggtc gatccttgct
     6841 acccgagcaa ggtctgccag gcgcacatcc acaacctgct gttcaagcac cacgagaaga
     6901 agccgctcaa ctacatcttc ttcccggcga tcacccacat cccgacgtac atcgtcaatc
     6961 agatggacac ggcgagctgc ccggtcgtct cgggcacgcc gaaggtgatc cgcgcagcgt
     7021 tcacgaagga gaccgacttc ttcgccgagc gcggcatcag ctacctcgac acggcggtca
     7081 cgctgaacga ggcgctgatg tgcaagaagc aaatgttcaa cgagtgggga ccgctgctcg
     7141 gcctcaccga ggacgagagc gacttcgcga tcgacgaagc gttcaaggcg gttcaggcgt
     7201 tcgaggacga gatggagcgc aagggcaagg agatcctcga cgaggtcgtc aaggacaacc
     7261 gcgtcgcgct cgtcatgctc ggccgcccgt atcacaacga tccgggcctc aaccactcgg
     7321 tgctcgagga gttccaggct ctcggttatc cgatcctgtc gatgcgctcg ctgccgcgtg
     7381 acaagccgac gctgaactgg ctgttcgcgg aggacctcgg caagaaggtg atcgagtccc
     7441 cgatggacgt caccgacgtc tggccggaga actactcgac gaactcggtg cagaaggtgt
     7501 gggcggcgaa gtacgccgcg cgtcacccga acctcgcgtg cctcgacctg tcgagcttca
     7561 agtgcggtca cgacgcgccg acgtacggtc tcatcgattc ggtcatcgcg agcgccggca
     7621 aggcgtactc ggcgctgcac gacatcgatg cgaacaagcc gagcggctcg atcaagatcc
     7681 gcgtcaagac gtacgcgcac acgctgatgc tgctgcgcga gaagctcgag gatcaggcga
     7741 cgaagaagac cgagctcgat cgcacgctgt cggcgaagaa gctcgagctc atgaagtcac
     7801 tgcaggcgaa gctcgctggc gtcggtcgca ccgacgacaa gctcgatgtc gaagtgaagg
     7861 cgctcgagac gcaggtggct gcgtgggcgg cggaagaagc agcgaagaac cccaaggcga
     7921 agaagctcgc ctctctcaat gtcatcaagg acggtagcgg cgctggctcc agcgctgccg
     7981 cgaatctcgc aaaggccaag gaagaacaat gagcacgacg aacaacaagt ccggtgagaa
     8041 tctcgacccg atgtccctcg aggccatcga ggaacagctg aagcagttcg agatcgacga
     8101 gcgcaagcgc cttggcctgc ctgtcgaagc gaccaagcac tggctcgacg aggtcccgcg
     8161 tcagttcacg cgcgatcagc gcgagcacac cacgatcctc gtgtcggggc tcacgatggc
     8221 gcacgacctg ttcattcagg cggcgctgcg cggcatcggc tacaaggtcc tcgcgatgga
     8281 cgtgccggat aacgacgcgc tccagtacgg acgcgagtac ggcaaccgcg gccaatgcaa
     8341 cccgacgtac ttcacggtcg gcaatctcgt gaagtacctc gatggcctgc gcgaccgcgg
     8401 catgtcgaag gacgacatcg tcaagaacca cctgttcatc acggctggcg cgtgcggccc
     8461 gtgtcgcttc ggcacgtacg tcaccgagta ccgcaaggcg ctccgtgact ccggcttcga
     8521 cggcttccgc gtcctgctgt tccagcagac cggtggtctc aagcaggcga cgggcgaggg
     8581 tgtcggtctc gagatgaacc cgacgttctt ctggggcgtc gttcgcgcga tcatgatcgg
     8641 cgacgcgctc aacgcgctcg gctaccgcat tcgtccgtac gaggtcgagg cgggcgccac
     8701 cgaccgtgcg atcgagtcga gcaagaagat catctccgac gcattcgaga acaacacgtc
     8761 gctgccgatg gcgctgtggc gcgtccgccg cacgatgagc aaggtcaagg tcgaccgcac
     8821 gaagccgaag ccgcgcgtcg gcatcatcgg tgagttctgg gcaatgacga cggaaggcga
     8881 cggcaactac ggcctgcagc gtttcctcga ggccgagggc gccgagccgg agatccagct
     8941 cgtcaccgcg tggctgctct acatgatctg gcagggccgc tacgacaccg cgcagcgcgc
     9001 gagcctccgc ggcgccgaca aggcgttcaa ggatgccgac ggcaagcagc aggtcggtgg
     9061 atcgaagttc agcctcgccg gcgtcgacgt gcgcaagcgc atcgcatcgc tgtgggcggg
     9121 cgagcaggtc ctccgtggtc tgttccagac cttcgcgcac gcgatgggtc tgtacgacta
     9181 ccacctgccg gacatgaacc agatcgccga gatctcgcac gcgttctacg acaacaacct
     9241 ccgtggcggc gaaggccaca tggaagtcgg caagctgatc cagaacgtcg cgtaccagaa
     9301 ggtcaacatg accctctcgg tgaagccgtt cgggtgcatg ccctcgtcgt cggtgtcgga
     9361 cggcatccag tcgttcatca ccgagctcta tccgcagggc atcttcctgc cgatcgagac
     9421 gaacggcgac ggcgcggtca acgtctacag ccgcgtccag atgcagctgt tcaaggccaa
     9481 gcagctcgcg cagaaggaag tcgacaaggc gctcgccgac gtcggtatga cgatcgagga
     9541 agtccgcacg tacctcgaca agcacccgtc gctcgcgcac gcgttccacc gcgcgccgca
     9601 caaggccggc tgcacggccg ctgacctcat ctacgaagtc ggcgcgcgca agaaccgcct
     9661 gcgctacgcg aaggacagcg tcgtcgccaa gctcacgggc aaggcgaaga aggagcacca
     9721 cgacgcgcac gtccagctgc tcgcgatcgc ccgcaaggcc gccgagaaga cgatgcgcaa
     9781 gaagagcgtc gtcacggtcg cgcccgagct cgacccgaac gatgactccg gccgcccggc
     9841 cgtgccggcg ggccgcaaga gcctccgcgt tgtgaactag tccacgtaac ctcgactagc
     9901 ttggcgaagc ccgtggtgtt gccgcaccac gggctttgtt tttggggaaa gctgatgcga
     9961 cggatagacg ggcttgctat tggtagcggt ctcatgtgca tcaatcggtt gcgccagttg
    10021 gttgtcgtca ctctcgtgac tgctgcatgc ggcaacagtg gcgacgacgg gaccggcggc
    10081 aatagcgggt tcaatgtcga caagctcttg cgagcatgca cggttctgca ctcgtgcttc
    10141 gtcggatacg agtacgagga catcggcacg tgcgtgtccg acttccagtg gcacgggacg
    10201 ccagctcaag tcgattgcgt gctggcggcg tccgtcgcga actgtagtgc cgccgagaca
    10261 tgcatcggcg agaagctcgc tgcggacacg gactgcgtgg atcgctgcct ggacggccga
    10321 acgttagtgc ggtgcaacgg cccgtccatg caccgagaga tggattgccg gacgtatctg
    10381 cttagcggag ggcctcaatg cctcgtcggt acggcagggg tcgtcgcatg cggaacgggt
    10441 acatgctcgg ccgatggcga ctcgacatgc gacggctcga tcctgcacag gtgcgggtcg
    10501 gggatcgcta cagatttgga ttgtgcgcct gtgggccttg agtgcagcac caacggctgc
    10561 gcgcggccgt cgccaggtgc gtgcacaatc cagttcggat gccagggcga cctgctctgt
    10621 aacgccaata ccaccgagga ttgcagcaag gtgattccgg acggaacgtg catcaccgtc
    10681 aatagcttca ccgcatgtgg ctgggccacg gagtgcgatc cttcgacacc acagacctgc
    10741 atcggcacta cgctgcagtc gtgcgtcctc ggcgcgctca agactgtcga ctgcacgacc
    10801 attggcggca cgcagtgcgt cccgaacacg caggcgccgg gcgcctggtg ctcgcggtga
    10861 acgcggccgc ctgacggcgt gctacgacag cagccggcat ggagcctcgc gatttctcga
    10921 cgatcggacc gtccgcgcgg atgctgctcg tgatgcgcgc gcaaagcgat ctgccgttcg
    10981 cgcggcaatc cgccgagctg ctgctaggag cggagcgggt cgcacaggat atcgcgcgcc
    11041 tggcggagct cgccggctct gcactgcgcc tcaggcactt cgtggcccgc taccgcagca
    11101 tcgatacgct gctcgcggag agcggcgcca tacgcgtgct cgagctcggc ggcggactct
    11161 cgctgcgtgg cctcgcctgg gcagagcgca acgccggcac gtacctcgac accgatctgc
    11221 ccgacatggt ccggaccaag cagcagctca tggacgcgct tgccgtcggg ccactcgtgg
    11281 gcgagctgcg gctcgccgcg ctgaacgcgc tcgatgccga tgcgtttcgg gcgtcggttg
    11341 cgaccttgcc ggcaggaccg atcgcgatcg tgaacgaggg cctgctgatg tacctcgacg
    11401 atgccgagaa gcagcagctc gccacgaaca tccgcgacgc gctttctgcg cgcggcggcg
    11461 tgtggatcac cgccgacatc tatgtccagg gcgaacgcag cgcgcgaatc gagcgtagcg
    11521 agcgcgagcg cgagttcctc gccgcccatc gcgtcgagga gaacaagttc gcgagcctca
    11581 ccgcggcaga atcgttcttc accggcgccg gcttcacgat cgtgcgacgg ctgccgtccg
    11641 acgtgacccg cgaatcatgg gtcgtcgagg cgcgctaatt gcgatatgaa cgcgccatga
    11701 gtttgaccga tcgacttcgc gcacgcgcgc acgccgccct cgagcgcgta cccggcaagg
    11761 taggtgacaa gctgcgcggg gcgaacgaag cgctcggccg gccgttcgca gacgcgaccg
    11821 agctcgccga tcgtcgcgcg ttcgatacgc gcggtggtgt gcaccaatcg gctgcacccg
    11881 cggccgcgaa ggcccagcgc gagcaagcac cggtgatcgt ctactacatg gagaaacaga
    11941 aacgcgacgt gtcgaagctg accgatgtgc tcgacgcgaa cggcattgcc tacacggcga
    12001 ctaacatcca ggaagatccg gccgcgcaat ttgccgtgcg gcgagatagc aagggtatcc
    12061 gcctgcctgt ggtcttcgtc gcaggcgact gcatcggtgg gcgtatcgag ctcatcaatg
    12121 cggctagctc cggagagctg aagaagaagg tgttcggcag ctgagatcgg gaaggtttgg
    12181 gcgggtttga cgtaataatc gaccaccatg atcggcacgc aggagcgcaa ggccggacag
    12241 gccgagtcgg ccgcgccatc ggctgagaat gaagcgccga acgccgcgcc atcgcatcaa
    12301 cgcttcacga acgaaggtcc gattccgccg gtcgcgcagt cgtcgaatgc ggtcgcaccc
    12361 ggcgcatcgg acctcgcgtt cggcgcgcac gacgagcact acgcgacggc cgacaactcg
    12421 ctcgacgacg ccaatcacct gcgcgtgaag gcgacgggcc tcaacgttcg ctcgtcgccg
    12481 aacgcaagag gtcacgcgaa tatcgtcggc ttgttgcacc acggcgatca gatcgaacgc
    12541 gtcggcaaag cagggcattg ggtgcagatc agctatcaag gtcagcaggc gttcgtgcac
    12601 ggcgcatacg tcgaggcagc gcaccacgcc gaggcaaagc ctgccaaagc accgtcggag
    12661 ccacagccag aatcggtggc caacgccgtc gagaaagcga cgcagcacgc gcccgagatc
    12721 aagcagcacc tgccggagaa gaaggtcacc gagaacgcgc cgcacgcgtc ggcgacccat
    12781 gcgcctagcg agacgaaggc gccggagtcg aagccagcag ccgagaaccc ggcgtcgaca
    12841 gcgccggtga tcaaccatgc gcccgtgagc aacgctcccg tcgccaa
//
LOCUS       DASUFX010000502         4152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1330506, whole genome shotgun sequence.
ACCESSION   DASUFX010000502 DASUFX010000000
VERSION     DASUFX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330506"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFV99_20130"
     CDS             <1..784
                     /locus_tag="VFV99_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061691.1"
                     /translation="LYANHWHRDARKLLTHHVPELRGRPVWMFSSGPLDESANAKELP
                     APHQLTKLIARIGARGHVTFGGRLERDAKGFIAGAMAKKLSGDWRDWHKVRAWAKDVA
                     HEILDEAPRPVVIMPTPSRAQRWLLAAMCLFVGITAILGGATLTARPDGSLMEAPPGM
                     LSHSPFTTFLIPGLLLLLIVGLGNAVAGVLVVRNARAAPYFAFFAGTALAVWIVTQMI
                     LLRTHHWLQIGYLTVAALILFEAWKVFELPWRHGRAARYATR"
     gene            753..1610
                     /gene="purU"
                     /locus_tag="VFV99_20135"
     CDS             753..1610
                     /gene="purU"
                     /locus_tag="VFV99_20135"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX5061692.1"
                     /translation="MAEQLGTLLVSCPDQKGIVAALAQVLYGQGANIVDSDQHTDPIA
                     GMFFQRICFDLSTAETDPQKLEAALRDISNRFELTYRLIVDAHTPRVAIFCSKQEHCL
                     YDLLIRYRAGELPCHVAMIISNHPTLEPVAKHFDVPFVHLPITAATKAQQEAAAIALL
                     ERENVDLVILARYMQILSPEFVARYPSRIINIHHSFLPAFIGANPYRQAYERGVKLIG
                     ATSHYVTSELDQGPIIEQAVVRCSHRDHVDELVRKGKDLEKRALARAVRLHLQDRILV
                     YSGKTVVFD"
     gene            complement(1607..2074)
                     /locus_tag="VFV99_20140"
     CDS             complement(1607..2074)
                     /locus_tag="VFV99_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061693.1"
                     /translation="MTRTTILALVLAACGRSESHRPAARDIQLRPDLSHATQADLATE
                     LDQAERRGTWREVRQRWEGQQLHWTVTRHEPLCRSASACNVAAFPVQQPATHGWLPEL
                     SFAPGELEKLTAACGNEDCQLEFIGTLSRLELSGDLPTSVHLDHVRVVGARKG"
     gene            complement(2089..3345)
                     /locus_tag="VFV99_20145"
     CDS             complement(2089..3345)
                     /locus_tag="VFV99_20145"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX5061694.1"
                     /translation="MKKRFLTIAGLSLAAGAITATASSHREAPMIGEDQFADNTDVYA
                     FISPTDANKLVLVADYVPLLLPQSGPNFYRFAEDAQYDINIDNDGDAVPDWTYRYEFE
                     TAVKNGNTFLYNVGPVDSLQSQNLNVTQTYSLWKINRKNGSRQRIMSGAPVAPWNVGK
                     RSFPNYEPVALQAIREANGTKSFAGPRDEPFFVDLHVFDLLGVGGAPTTDGVNVMSLV
                     LEVPITELAKGGTRPTSTTDKTSVVGIYASASRPQIRILRKTRDSDDYGKMIQVSRLG
                     WPLVNEVIVPLKDKDKYNRSEPKDDVANFGAYILDPEVPKLLNLVLDAGCPETPSGGR
                     TDIVGLLAPNGTKPADLLRINIAEGQTNAQSHFPNGRALADDVTDTLLTVACNNNAAI
                     GDGVNANDKPFTTTFPYLASPHSGNP"
     gene            complement(3358..>4152)
                     /locus_tag="VFV99_20150"
     CDS             complement(3358..>4152)
                     /locus_tag="VFV99_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061695.1"
                     /translation="TSPTDAVASLDGREFYFAAYDLEKQPALFRTASTAGATAEKIAA
                     GDPLESPLGLVLSCDGKTLYIADIGGDAGAVLSVGTSGGTPGDLAVTGIVRPGGLAMG
                     PDCKTLFATGRTEDGKPALFKISVTGGVALPVWVGEPLVSPSGLHVDDDGVAWVMDHR
                     AGGEHGFGVLFAIPEDGSAANEVISDLRMGTFGGVSLTAGGGTAVMPTIDAEGHGQLT
                     SVEIASGTVQQLATPELIDPSGLRTARNAGVFAVVDSEGGAIYSAK"
BASE COUNT          687 a         1368 c         1349 g          748 t
ORIGIN      
        1 gctgtatgcg aaccattggc atcgcgacgc gcgcaagctg ctcacgcatc atgtacccga
       61 gctacgcggt cgcccggtgt ggatgttctc gagtggcccg ctcgacgagt cggcgaacgc
      121 caaagagctg ccggcaccgc accaactcac gaagctgatc gctcgcatcg gcgcgcgcgg
      181 ccacgtcacg tttggcggcc gcctcgagcg cgacgcgaag ggattcatcg ccggcgcgat
      241 ggcgaagaag ctgagcggcg actggcgcga ctggcacaaa gtccgtgcgt gggcgaagga
      301 cgtcgcgcac gagatcctcg atgaagcgcc gcgcccggtc gtgatcatgc ctacgccctc
      361 acgcgcacag cgctggctgc tcgcggcgat gtgcctgttc gttggcatca cggcgatcct
      421 cggcggcgcc acgctcaccg ctcgccccga cggctcgctg atggaagctc ctcccggaat
      481 gctgagtcac tcgccgttca cgacgttcct gatcccgggt ctcttgctgt tgctgatcgt
      541 cgggctcggc aacgcggtcg cgggcgtgct cgtcgtgcgc aatgcacgag cggcaccgta
      601 cttcgcgttc ttcgcgggca ccgcgctcgc cgtgtggatc gtgacgcaga tgattttgct
      661 tcgcacccat cattggctcc agattggcta cctcaccgtc gccgcgctca ttctgttcga
      721 ggcgtggaag gtgttcgagc taccatggcg gcatggcaga gcagctcggt acgctactcg
      781 ttagctgtcc ggaccagaaa ggtatcgtcg ccgcgctcgc ccaggtgctc tacggccaag
      841 gcgccaacat cgtcgattcg gaccagcaca ccgatccgat cgcaggcatg ttcttccagc
      901 ggatctgctt cgacctatcg accgcggaga cagaccccca aaagctcgaa gcggcactgc
      961 gcgacatcag caaccgcttc gagctcacgt accggctgat cgtcgacgcg cacacaccac
     1021 gcgtcgcgat cttctgctcg aagcaggagc actgcctcta cgacctgctc atccgctacc
     1081 gcgccggcga gctgccgtgc cacgtcgcga tgatcatctc gaatcacccg acgctcgagc
     1141 ccgtcgcgaa gcacttcgac gtgccgttcg ttcacctgcc gatcacggcg gcgacgaagg
     1201 cgcagcaaga agctgccgcg atcgcgttac tcgagcgcga gaacgtcgat ctcgtgatcc
     1261 tcgcgcgcta catgcagatc ctgtcgcctg aattcgtcgc acgctacccg tcgcggatca
     1321 tcaacatcca tcactcgttc ttgcccgcat tcatcggcgc gaatccgtac cgccaggcgt
     1381 acgagcgcgg cgtgaagctg atcggcgcca cgagccacta cgtgacgtcc gagctcgatc
     1441 aaggaccgat catcgagcag gcggtcgtgc gctgctcgca tcgcgatcac gtcgacgagc
     1501 tcgtgcgaaa gggcaaggac ctcgagaaga gagcccttgc ccgtgcggtc cggctacatc
     1561 tgcaggaccg gatcctcgtg tactccggca agaccgtcgt gttcgactag cctttgcgcg
     1621 cgccgaccac gcgcacgtga tccaaatgca ccgacgtcgg caggtcgccc gacagctcga
     1681 gccgcgacag cgtgccgata aactccagct ggcagtcctc gttgccacac gcagccgtga
     1741 gcttctcgag ctcgccgggt gcgaacgaca gctcgggcaa ccagccgtgc gtcgccggtt
     1801 gctgcaccgg aaacgccgcg acattgcacg cgctcgccga cctgcacagc ggctcgtgcc
     1861 gcgtcacggt ccaatggagc tgttggcctt cccagcgctg ccgcacctcg cgccacgttc
     1921 cgcgccgctc cgcctggtcg agctcggtcg cgagatcggc ttgcgtcgca tgcgagagat
     1981 cgggccggag ctggatgtcg cgtgccgccg ggcggtgcga ctcgctgcga ccacaggcag
     2041 cgagcacgag agcgagaatc gtggtgcggg tcatggcgtg ctccgaggct aggggttacc
     2101 ggaatgcggc gacgcgaggt acgggaacgt cgtcgtgaac ggcttgtcgt tcgcgttcac
     2161 gccgtcgccg atcgccgcgt tgttgttgca cgcgaccgtg agcagcgtgt cggtgacgtc
     2221 gtcggcgagc gcgcggccgt tcgggaagtg cgattgcgcg ttcgtctggc cctccgcgat
     2281 gttgatacgg agcaggtccg cgggtttcgt gccgttcggc gcgagcaggc cgacgatgtc
     2341 ggtacgacca cccgacggcg tctccgggca accagcgtcg agcacgaggt tgagcagctt
     2401 cggcacttcc ggatcgagga tgtacgcgcc gaagttcgca acgtcatcct tcggttcgga
     2461 gcggttgtac ttgtccttgt ccttgagcgg tacgatcact tcgttgacga gcggccagcc
     2521 caggcgcgaa acctggatca tcttgccgta atcatccgag tcgcgcgtct tgcgcaggat
     2581 gcggatctgc ggccgcgacg cgctcgcgta gatgccgacg acggaagtct tgtcggtcgt
     2641 cgaggtcggc cgcgtgccgc ccttcgcgag ttcggtgatc ggcacctcga gcacgagcga
     2701 catcacgttg acgccgtcgg tggtcggtgc gccgccgacg ccgagcaagt cgaacacgtg
     2761 gaggtcgacg aagaacggtt cgtcgcgtgg accggcgaac gacttggtgc cgttcgcttc
     2821 gcgaatcgcc tgcagcgcga ccggctcgta gttcgggaac gagcgcttgc cgacattcca
     2881 cggcgcgacc ggagcgccgc tcatgatgcg ctggcgcgag ccgttcttgc ggttgatctt
     2941 ccagagcgag tacgtctgcg tgacgttgag gttctggctc tgcagcgaat cgaccggacc
     3001 gacgttgtag aggaacgtgt tgccgttctt caccgcggtc tcgaactcgt agcgatacgt
     3061 ccagtcgggg accgcgtcgc cgtcgttgtc gatgttgatg tcgtactgcg cgtcctccgc
     3121 gaagcgatag aagttcgggc ccgattgcgg caagaggagc ggcacgtagt ccgcgacgag
     3181 cacgagcttg ttggcatcgg tcggactgat aaacgcgtag acgtcggtgt tatcggcgaa
     3241 ctggtcctcg ccgatcatcg gtgcttcgcg atgactcgac gcggttgcag tgatggcgcc
     3301 ggcggcaagc gataagccgg cgatggtgag aaagcgtttc ttcatggctg gtccctttca
     3361 tttggcgctg tagatcgcgc caccctcgga atcgacgact gcgaacacgc cggcattacg
     3421 cgcggtacgc agacctgatg gatcgatgag ctccggcgtt gcgagctgct gcactgtgcc
     3481 gctcgcgatc tcgacggagg tgagctgacc gtgaccttcg gcatcgatgg tgggcatcac
     3541 ggccgtgcca ccaccggcag tcagcgatac gccgccgaac gtgcccatgc gcagatcgct
     3601 gatgacttcg ttcgccgcgc tgccgtcttc tggaatcgcg aagaggacgc caaaaccatg
     3661 ctcgccgccc gcgcggtgat ccatgaccca cgcgacgccg tcgtcgtcga catggagacc
     3721 gctcggcgac accagcggct cacccaccca gaccggcagt gctacgccgc cggtcacgga
     3781 gatcttgaac agcgctggct tgccgtcttc ggtgcggccc gtcgcgaaca gcgtcttgca
     3841 gtccggtccc atcgcgaggc cgcccgggcg cacgatgccg gtcactgcga gatcgcccgg
     3901 tgtgcctccg ctcgtgccga cggacagcac ggcgccggcg tcgccgccga tatcggcgat
     3961 gtagagcgtc ttgccgtcgc acgacagcac gaggccgagc ggcgattcga gcggatcgcc
     4021 ggctgcgatc ttctcggccg tcgcacctgc ggtggacgcg gtacggaaca gcgcgggctg
     4081 cttctcgaga tcgtacgcag cgaaatagaa ttcgcggcca tcgaggctcg cgaccgcatc
     4141 ggtcggcgac gt
//
LOCUS       DASUFX010000503         7996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1885948, whole genome shotgun sequence.
ACCESSION   DASUFX010000503 DASUFX010000000
VERSION     DASUFX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885948"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFV99_20155"
     CDS             <1..404
                     /locus_tag="VFV99_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061696.1"
                     /translation="GGYCVVSSCPRDCTECDEVEKTCTAECTSAASCNNVTCPTGWDC
                     TIHCTGANACADITCESDSKCAITCSGTDACETVFCTAACKCDLDCAAGACNTPSCPT
                     VGNGGNQVQCTTDGTNTSNCDSGHAAGCATC"
     gene            433..939
                     /gene="greA"
                     /locus_tag="VFV99_20160"
     CDS             433..939
                     /gene="greA"
                     /locus_tag="VFV99_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5061697.1"
                     /translation="MSEKFPMTPTGYASMKAQLSKLKNVDRPANSRAIEVARGHGDLS
                     ENADYSAAKEEQGLIEAKIRELEAKIAMAEVIDPTKLSGSRVKFGATVTIEDTDSGET
                     QRYTIVGEHEADIKKGRISIGAPVARALIGKEVGETVTVNNPKGKREYEVTEVEWLEV
                     PALEEVTS"
     gene            936..1169
                     /locus_tag="VFV99_20165"
     CDS             936..1169
                     /locus_tag="VFV99_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061698.1"
                     /translation="MKRLVLMIAVLVSAVYVGCKQGDGDRCQVNEDCESDICNVAKGT
                     CAPAGGEMEEIDAGVPDGPDAAPDAPSDAPGDV"
     gene            complement(1166..2281)
                     /locus_tag="VFV99_20170"
     CDS             complement(1166..2281)
                     /locus_tag="VFV99_20170"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5061699.1"
                     /translation="MRTLAAVAVATMIGGIALAGERAGKVVRVEQTPKREVFVPAGAF
                     WMGVTEDDIKVVKELCVQFFEPDSVQLIGQRAAPTLCGGYEEELDNMKLRQVFLSAFA
                     IDRYEVTVADYRQCVTAGRCNLDPLIAGDERYIRNEWPIVNVTWNEAQEYCRWRGGRL
                     PTEAEWERSARGAEVGSVADQADQDRAASLQWPWCHEKYQACGAKKGQARQDCLNTKC
                     VERPKDFNHGQPRSQAIREVDRSGLTLHLLGDPDDVDGYALLAPPGQYPWGEGPFGTR
                     DQAGNVAEWVADARGATDALAGYAELPGCATIDDEIRCINPKREGNDRDLRIVRGGSW
                     RQPSFLGRVNVRDPFGPVYDPRRRFSHVGFRCARSVD"
     gene            complement(2321..4006)
                     /locus_tag="VFV99_20175"
     CDS             complement(2321..4006)
                     /locus_tag="VFV99_20175"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5061700.1"
                     /translation="MRCIALVIVLAACGPKGPPPASPSTVPRLLAPPLIDPEVRGAAY
                     LTAVALSLQPAWHQFLEDCRLRLPADHALNHMDLVTTAQLEIGARGELAKLAIAPSGN
                     RDFDKAIEQVVRDAVPLPPPPRELWSDDDRVHLAWTFARDRRQAGPATAQVGDVLLPL
                     RVVVAQRIRQNDLTRAARRIAREPVGAERDAATQRLMIAALDEGARSSDVGVRKAAVQ
                     AIATAGVHELDTVAADLVKSADLDLSSAVIEHGFAPTNVLALLFHRELTQAPKRTRLI
                     IAALAVRDHAAAVDLVRRELATTPATDPAMLQALAIAPVPDVADKLVSAFGRGDASQR
                     SGVCGALARYPAAVALPIVTRGLGDRDASVRASCLATLREQATAAKAKPALLTRVREL
                     AKDRDSLVRAQAIAALAAIAPATLPDAVDDRAADVRAAYAGALALVPRSDATTPLVTL
                     ADDRDPDVRAAAWTVLAAGSSAQTANLAKHAAKDSAAQVRLAALPAARDEDLLLELAN
                     RDDSTDVRTAALVQLARIRTRHAIADLLLARLADAAPGSAERVRTALAWLQAR"
     gene            complement(4026..4802)
                     /locus_tag="VFV99_20180"
     CDS             complement(4026..4802)
                     /locus_tag="VFV99_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061701.1"
                     /translation="MSKLLKVACLIGAAQGFGACGGGVDDPCPTNDCELKGRTTIKWT
                     LDAYPDRGFAMDSCADFGVWKVSVDLVGANGVVTSGRDDCGVAQATFSGLEPGDYDVY
                     VMPLDAGGTPLLLNPIIGKVTAGTVEQPQETTINVPWDQWIGTFTGTYLFRLSWGGLS
                     CEQAMPEVKNQTLTLTVNGVVQNLVTDDGQMLNGSDKKPCKKLSDNFPQSALDVAFGP
                     ATLLVEGFDATDNMMFSKQFDTFVGAGITNPTLMFDVPLQ"
     gene            complement(4799..>7996)
                     /locus_tag="VFV99_20185"
     CDS             complement(4799..>7996)
                     /locus_tag="VFV99_20185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061702.1"
                     /translation="AEKLGDLEGAIQRYEAAVAANGSDAEALKALVDLYDKTGRTEDY
                     LRTMERLGAVAPEGEKLQTLRKLAAELEERDPTRARDAYEKLLAADPNADDAYRGLER
                     ELKGEANWYELVAVYNRHIAAIKTPAARVELYLEAAQIHERELDDPHKAIDCLMNVTA
                     IDDKNKTALAVLPRLYVRTEAFDRAVDVLVKHAALEGDKGAQLYAEAGRISFENLMDP
                     EIAQRHLEKALSLDNENLEALKVLGHLHEKRGNWTQSVEMLLRAEAASGSRSERINLL
                     WDAALITDQRLEDPSRALELFERVLKLDPDHVDAGQRVADRLVANKRWDDALPVLEML
                     ARQAEGLDRLERSRRESQLGNAYEALHRYEKAARHFRLAVESDPESLDAAIGLANVLM
                     AEAKVHEGSEQAAAMWQEVEKRYRELLARHRTGMADGHVAEIWYRLGAASRALGEDKK
                     AEASFRRALEKEPLHEPTLEAMVELGGARGEWRMVADAKRAQIDALSKRDGTDYNRAK
                     LFEEIGDTWRERIKDVANATGAYLEGLKIAPASRVLLHKLLEAFTEQRQWRRSIETLD
                     QLSGLEDSAERRARFHYTAAVIARDELHDQELAVDKFNAALDDNPMTSKAFEGIDAVL
                     TSRKDWKNLARAYRRQLKRVHEDAPTEKLVELWTRLGDICADHLGDTEAATEAYQVAC
                     ELAPDDVSRHEQLADLYLEAGEARRAEAINELQYLLAHAPDRVELYKALANLYRAEHE
                     LDKAFCVAQALVFLGAASTEERMLYEKFRPRSFTPAPRRLTEELWQKALIHPREDKAV
                     GAIFASTLGALAAGSAQPITAFGLSPESRADLDRDPRPVSRVIKYVAGVLAIEPAPMV
                     WLQEQGDGLRVANTVGLGADRQKLVPSLLVGAPVIGKGDERELAFEVGKRMAYLRAER
                     FVTLAVGTLPKLEAAFAAAVLASGARLTGHDGQPYNPDNEDAKKLAGQLMRQVPGPLL
                     EQVGELSSKLTGRLGNGLIAGWRAATDLTANRVGFVLANDFEVAAKGIATEGATLSSM
                     SVKERLRELLAYAVSEQYFAVRRHLGLHVRGEVSA"
BASE COUNT         1283 a         2711 c         2671 g         1331 t
ORIGIN      
        1 ccggcggcta ttgcgtggtg tccagctgcc cgcgcgactg caccgagtgc gacgaggtcg
       61 aaaagacctg cacggccgag tgcacgagcg ccgctagctg caacaacgtc acgtgcccga
      121 ccggctggga ctgcacgatc cactgtaccg gcgccaacgc atgcgccgac atcacctgcg
      181 agagcgactc gaagtgcgcg atcacgtgct ccggcacgga tgcgtgcgaa accgtattct
      241 gcaccgccgc ctgcaaatgc gacctcgact gcgccgcggg cgcatgcaac acgccctcgt
      301 gcccgaccgt cggtaacggc ggcaaccagg tccagtgcac gacggacggg acgaacacca
      361 gcaactgcga ttccggccac gccgccggtt gcgcgacgtg ctagacccgc agtacgaatc
      421 ggtgccgctg ccatgagcga gaaattcccg atgaccccga cgggttacgc gtcgatgaag
      481 gcacagctgt ccaagctgaa gaacgtcgac cgcccggcga atagccgcgc gatcgaggtc
      541 gcacgcggtc acggcgatct gtcggaaaac gcggattaca gtgccgccaa agaggaacag
      601 gggctgatcg aggccaagat tcgcgagctc gaagcaaaaa tcgcgatggc cgaggttatc
      661 gatccaacca aattgtcggg ttcgagagtc aaattcgggg ccactgtgac catcgaggat
      721 accgatagcg gcgagacgca gcgctacacg atcgtgggcg agcacgaagc cgatatcaag
      781 aagggccgga tctcgatcgg cgcgccggtc gcgcgcgcgt tgatcggcaa agaggtcggc
      841 gaaaccgtga ccgtgaacaa cccgaagggg aaacgcgagt acgaggtgac cgaggtcgag
      901 tggctcgaag ttcctgcgct cgaggaggtc acgtcgtgaa gcgacttgtt ctaatgattg
      961 ccgtgctcgt gagtgcggtc tacgtgggct gcaagcaagg ggacggcgat cgctgccagg
     1021 tcaacgagga ctgcgagagc gacatctgca acgtggccaa gggcacgtgc gcgccagccg
     1081 ggggcgagat ggaggagatc gacgccggcg tccctgacgg tcccgatgca gctcccgacg
     1141 ctccgagcga cgcgcccggc gacgtctagt cgacgctgcg cgcgcagcga aagccaacgt
     1201 gcgagaaccg acggcgcggg tcatagaccg gcccgaaggg atcgcgcacg ttcacgcgac
     1261 cgaggaacga cggctgtcgc cacgagccgc cccgaacgat gcggaggtcg cgatcgttgc
     1321 cctcacgctt gggattgatg cagcggatct cgtcgtcgat cgtggcgcag ccgggcagct
     1381 ctgcgtagcc ggccagcgcg tcggtcgcgc cgcgagcatc ggctacccac tcggcgacat
     1441 tgccggcctg atcgcgcgtg ccgaacggtc cttctcccca cggatactgc ccaggcggcg
     1501 cgagtagcgc gtagccgtcg acatcgtcgg gatcgccgag taggtgcagc gtcagccccg
     1561 agcggtcgac ttcgcgaatc gcctggctgc gcggttggcc gtgattgaag tctttcggcc
     1621 gttcgacaca cttcgtgttc aggcagtcct gacgcgcctg cccctttttc gctccgcagg
     1681 cctggtactt ctcgtgacac cagggccatt gcaggctcgc ggctcgatcc tgatcggctt
     1741 ggtcggcgac cgacccgacc tcggcgccgc gcgccgagcg ctcccattcc gcttctgtcg
     1801 gcagtcgtcc gccgcgccag cggcagtact cttgggcctc gttccaggtc acgttcacga
     1861 tcggccactc gttgcggatg taacgctcgt cgccagcgat cagcggatcg aggttgcagc
     1921 ggccagcggt gacgcactgg cgatagtcgg cgacggtgac ctcgtagcga tcgatcgcga
     1981 atgcgctgag aaacacctgg cgcaacttca tgttgtccag ctcttcttca tagccgccgc
     2041 acagcgtggg agccgcgcgc tgaccgatga gctgcacgga atcgggctcg aagaactgca
     2101 cgcacagctc cttgacgacc ttgatgtcat cctccgtgac gcccatccag aacgcgccgg
     2161 cgggcacgaa cacctcgcgc tttggcgtct gctcgacgcg cacgactttg cccgcacgct
     2221 cgcccgcgag ggcaatgcca ccgatcatgg tggcaaccgc gacggcggct agcgtacgca
     2281 tcacggcaag cgtgccgcga atcatcgcgt cgtgcaaagc ctagcgcgcc tgtaaccacg
     2341 caagagctgt gcgtacacgc tcggcgctgc ccggggcggc atcggccagc cgcgcgagga
     2401 gcagatcggc gatcgcatgg cgtgtgcgaa tccgggcaag ttgcacgagt gcagcggtcc
     2461 gcacgtcggt cgaatcgtcg cgattcgcga gctcgagcag caggtcttcg tcgcgggccg
     2521 cgggcagcgc tgcgagccgc acctgcgcag cagagtcttt cgcagcgtgc ttggccaggt
     2581 ttgctgtctg cgcactcgaa ccggcagcga gcaccgtcca cgcagccgcg cgcacgtctg
     2641 gatcccgatc gtcggcaagc gtcacgagtg gcgtcgtggc atcgctgcgc ggcacgaggg
     2701 cgagcgcacc ggcgtaggca gctcgcacgt cggcggcgcg atcatcgacg gcgtcgggta
     2761 acgtcgccgg cgcgatcgct gcgagcgctg cgatcgcctg cgcgcgcacg agtgagtcgc
     2821 ggtccttcgc gagctcgcgc acgcgcgtga ggagcgccgg ctttgccttc gctgcggtcg
     2881 cctgctcgcg cagcgttgcg aggcagcttg cgcgcacgct ggcgtcgcga tcgccaaggc
     2941 cgcgcgtcac gatcggcagt gcgacagccg cggggtaacg agcaagtgcg ccacacacgc
     3001 cactgcgttg actcgcgtcg cctcgcccga acgcgctcac tagcttgtcc gcgacgtcgg
     3061 gcaccggtgc gattgcgagc gcctgcagca tcgccggatc ggtcgccggt gtcgtcgcga
     3121 gctcgcggcg gaccaggtcc acggctgctg cgtgatcgcg taccgccagc gcggcgatga
     3181 tgagccgcgt gcgcttcggt gcctgtgtga gctcgcgatg aaacagcaac gccaagacat
     3241 ttgtcggcgc gaagccgtgt tcgatcaccg cgctcgacag atcgagatcg gcggatttca
     3301 ccaggtctgc tgccaccgta tcgagctcgt gaacgccggc ggtcgcaatc gcctgcacgg
     3361 ctgcctttcg cacaccgaca tcgctactgc gcgcgccctc gtcgagcgcg gcgatcatca
     3421 gtcgttgcgt cgcggcatcg cgctcggcgc cgacaggctc gcgcgcgatt cgccgcgcgg
     3481 cgcgcgtcag atcgttctgg cgaatccgct gggcgacaac gacgcgcagt ggcaacaaca
     3541 cgtcgccgac ctgcgcggtc gccggccccg cttggcgccg atcgcgcgcg aacgtccatg
     3601 cgagatgcac gcgatcgtcg tcggaccaca gctcgcgcgg cggcggcggt agcggaaccg
     3661 cgtcgcgaac gacttgctcg atcgccttat cgaaatcgcg attgcccgac ggtgcaatcg
     3721 ccagcttggc cagctcacca cgcgcgccga tctcgagttg cgccgtggtg acgaggtcca
     3781 tgtggttcag cgcgtggtcc gccggcagcc ggagccgaca atcctcgagg aactgatgcc
     3841 aggcgggctg cagcgacagc gccacggcgg tcagatacgc agcgccgcgc acctcggggt
     3901 cgatcagcgg cggcgccagc aaccgcggca cggtcgacgg cgacgccggc ggtggacctt
     3961 tcgggccgca cgccgcgagc acgatcacga gcgcgatgca ccgcacccgc gaatcgtagc
     4021 agggactact gcagtgggac gtcgaacatc agcgtcgggt tcgtgatacc ggcgccgacg
     4081 aacgtatcga actgcttgct gaacatcatg ttgtcggtgg cgtcgaagcc ctcgaccagc
     4141 agcgtggctg gaccgaacgc gacgtcgagc gccgactgcg ggaagttgtc cgacagcttc
     4201 ttgcacggct tcttgtcgct gccgttgagc atctggccgt cgtcggtcac gaggttctgc
     4261 acgacgccgt tcacggtgag cgtgagcgtc tgattcttca cctcgggcat cgcctgctcg
     4321 cacgacagcc cgccccacga gaggcggaac aggtatgtgc cggtgaacgt gccgatccac
     4381 tgatcccacg gcacgttgat cgtggtctct tgcggctgct cgacggtgcc cgccgtcacc
     4441 ttgccgatga tcgggttcag gagcagtggg gtcccgccgg cgtcgagcgg catcacgtag
     4501 acgtcgtagt caccgggctc gagcccggag aacgtcgcct gcgccacgcc gcagtcgtcg
     4561 cgcccgctgg tcacgacacc attcgcgccg accagatcga ccgagacctt ccacacgccg
     4621 aagtcggcgc atgaatccat cgcgaagccg cggtcgggat acgcatcgag cgtccacttg
     4681 atggtcgtgc ggcctttcag ctcgcagtca ttcgttggac atggatcgtc gaccccaccc
     4741 ccacacgcgc cgaaaccctg cgcggccccg ataagacaag ccacctttag tagtttgctc
     4801 acgcactgac ctccccgcgt acgtgtagac ccaagtgacg gcgaacggcg aagtactgct
     4861 cgctgaccgc gtatgcgagc agctcgcgca ggcgctcctt gacggacatg ctcgataggg
     4921 tcgcgccctc ggtggcgatg ccctttgcgg cgacttcgaa gtcgttcgcg aggacgaagc
     4981 caacgcggtt cgcggtgaga tcggtcgccg cgcgccagcc tgcgatcagg ccgttgccga
     5041 gtcgaccggt gagcttcgac gacagctcgc ccacctgctc gaggagcggg cccgggacct
     5101 gccgcatcag ctgaccggcg agcttcttcg cgtcctcgtt atcggggttg tacggctgac
     5161 cgtcgtggcc cgtgagccgc gcgccgctgg cgagcaccgc cgcggcgaac gccgcttcga
     5221 gcttcggcag cgtgccgacc gcgagcgtga cgaagcgctc ggcccgcaga tacgccatgc
     5281 gcttgccgac ctcgaacgcg agctcgcgct cgtcaccctt gccgatcacc ggtgcaccga
     5341 cgagcagtga cggcacgagc ttctggcgat ctgcgccgag gccaaccgtg ttggcaacgc
     5401 ggaggccgtc gccctgctct tgcagccaca ccatcggcgc cggctcgatt gcgagcacgc
     5461 cagcgacgta cttgatcacg cggctgaccg ggcgcgggtc gcgatcgaga tccgcgcgcg
     5521 actcgggcga aagcccgaac gccgtgatcg gctgcgcact acccgcggcg agtgcaccga
     5581 gcgtcgacgc gaagatcgca ccgaccgcct tgtcctcgcg cggatggatg agcgccttct
     5641 gccagagctc ctcggtgagg cggcgcggcg ccggcgtgaa gctccgcgga cggaacttct
     5701 cgtacagcat ccgctcctcc gtcgacgcag cgccgaggaa tacgagcgct tgcgcaacgc
     5761 agaacgcctt gtccagctcg tgctccgcgc gatagaggtt cgcgagcgcc ttgtagagct
     5821 cgacgcgatc gggcgcgtgg gcgagcaggt actgcagctc gttgatcgct tcggcgcgac
     5881 gcgcttcgcc tgcctcgagg tagaggtcgg cgagctgctc gtggcgcgag acgtcatccg
     5941 gcgcgagctc gcatgccacc tgatacgcct cggtggccgc ctcggtgtcg ccgaggtgat
     6001 cggcgcaaat gtcaccgagc cgcgtccaca gctcgacgag cttctcggtc ggcgcatcct
     6061 cgtggacacg cttgagctgg cggcggtacg cgcgagcgag gttcttccag tccttgcgcg
     6121 aagtcagcac ggcgtcgatg ccttcgaacg ccttcgacgt catcgggttg tcgtcgagcg
     6181 ccgcgttgaa cttgtcgacc gcgagctcct gatcgtgcag ctcgtcgcgc gcgatcacag
     6241 ccgccgtgta atggaagcgc gcacggcgct ccgccgaatc ctcgagcccc gagagctgat
     6301 cgagcgtctc gatcgagcgg cgccactgac gctgctcggt gaatgcttcg agcagcttgt
     6361 ggagcagcac gcgcgaggcc ggcgcgatct tgaggccttc gagatacgca cccgtcgcgt
     6421 tcgccacgtc cttgatgcgc tcgcgccagg tgtcaccgat ctcctcgaac agcttcgcgc
     6481 ggttgtagtc ggtgccgtcg cgcttcgaga gcgcgtcgat ctgcgcgcgc ttcgcgtcgg
     6541 cgaccatgcg ccactcgccg cgtgcaccac cgagctcgac catcgcttcg agcgtcggct
     6601 cgtgcagcgg ctccttctcg agagcacggc ggaagctcgc ttctgccttc ttgtcctcac
     6661 cgagcgcgcg cgacgcggcg ccgaggcggt accagatctc ggcgacgtga ccgtcggcca
     6721 tgccggtgcg gtgacgcgcg agcagctcgc ggtagcgctt ctcgacttcc tgccacatcg
     6781 cagcggcctg ctcgctaccc tcgtgcacct tggcttccgc catcagcacg ttcgcgaggc
     6841 cgatcgcagc gtcgaggctc tccggatccg actcgacggc gagccggaag tggcgtgctg
     6901 ccttctcgta gcggtgcagc gcttcgtacg cgttaccgag ctgcgactcg cggcggctgc
     6961 gctcgaggcg atcgagaccc tcggcttgcc gcgcgagcat ctcgagcacc gggagagcat
     7021 cgtcccaccg cttgttcgcg acgagtcgat cggcgacgcg ctgaccggcg tctacatgat
     7081 cgggatcgag cttgagcacg cgctcgaaca gctcgagcgc ccggctcgga tcctcgagtc
     7141 gctgatccgt gatcaacgcg gcatcccaga gcaggttgat gcgctcgctg cggctgccgc
     7201 tcgcggcctc cgcgcgcagc agcatctcga ccgactgggt ccagttgccg cgcttctcgt
     7261 gcaggtgacc gagcaccttg agcgcttcga ggttctcgtt gtcgagcgac aacgccttct
     7321 cgaggtggcg ctgtgcgatc tcggggtcca tcaggttctc gaacgagatg cggcccgctt
     7381 cagcgtagag ctgtgcgccc ttgtcgcctt cgagcgccgc atgcttgacg agcacgtcga
     7441 ccgcgcggtc aaacgcctcg gtgcgcacgt agaggcgcgg cagcaccgcg agcgccgtct
     7501 tgttcttgtc gtcgatcgcc gtgacgttca tcaggcaatc gatcgccttg tgcggatcat
     7561 cgagctcgcg ctcgtggatc tgagcggcct cgagatagag ctcgacgcgc gcggccggcg
     7621 tcttgatggc ggcgatgtgg cggttgtaga ccgcaacgag ctcgtaccag ttcgcctcgc
     7681 ccttgagctc gcgctcgagc ccgcggtacg cgtcgtcggc gttcggatcg gctgcgagca
     7741 gcttctcgta cgcatcgcgc gcgcgcgtcg gatcgcgctc ttcgagctcg gcggcgagct
     7801 tgcgcagcgt ctgcagcttc tcgccttcgg gggcgacagc gccgagacgc tccatcgtgc
     7861 ggaggtagtc ctcggtgcga ccggtcttgt cgtacagatc gacgagcgcc ttgagcgctt
     7921 ccgcgtcgct gccgttggcg gcgacggctg cctcgtagcg ctggatcgcg ccttcgaggt
     7981 cgccgagctt ctcggc
//
LOCUS       DASUFX010000504        13482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1609975, whole genome shotgun sequence.
ACCESSION   DASUFX010000504 DASUFX010000000
VERSION     DASUFX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609975"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFV99_20190"
     CDS             <1..769
                     /locus_tag="VFV99_20190"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX5061703.1"
                     /translation="DAAWTATNPSGVAVLRKRRAGWQCLALAPSYASFVALADGTPVD
                     WGARPAAAIAPPDALLAATERLAGARPDVIAIDMPLATTRIVARRAADNAIASAYATR
                     GLGAHSPNESRPGPIAHAMRQGFSAHGYALATSATPAGTRTVMIEVFPHAAAIALLGA
                     SYRVPYKLARVTQYWPDRSPPQRRRTLLAQWTKLRRALAGHVLDVELRVPATGTLSAL
                     KRYEDALDAVLCAWSGAEYLARRTRAYGDATAAVWAH"
     gene            complement(766..1281)
                     /locus_tag="VFV99_20195"
     CDS             complement(766..1281)
                     /locus_tag="VFV99_20195"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5061704.1"
                     /translation="MTRAAVVALLIAPTARADDPKKKPAKNEPQAQEKMEKGGAVFIT
                     PDALKWGEPPPDVPKGAKLAVLYGDPSKPGMFTMRIKAPDGYKIPAHWHSNDEHLTII
                     SGTLQMYMGDTMTGEPHKLTAGSYHFLPAKMHHGAQTKGETIVQVSGPGPFDIHYINP
                     ADNPNPKSAKR"
     gene            1409..2365
                     /gene="msrP"
                     /locus_tag="VFV99_20200"
     CDS             1409..2365
                     /gene="msrP"
                     /locus_tag="VFV99_20200"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX5061705.1"
                     /translation="MTKSLENEVTPPEVYFNRRAILRAGAIAAGALGTGILYRKLNGV
                     DLVQTQTAEVSGVVKPTDPAYAVAEAKTPYESITNYNNFYEFTTNKDGVAKAAAGFKT
                     NGWAVEVGGLVHKPKVFDMDAIRRLSAPEERIYRMRCVEAWSMVVPWVGYSLSKLLAV
                     VEPMASAKYVAMQTLLDVSRMPGQANDVLDWPYVEGYRIDEAMHPLALLTTGLYGREL
                     PPQDGAPVRMIMPWKYGFKGIKSIVKITLVDKQPPTSWNKYAANEYGFYANVNPNHPH
                     PRWSQATEQRIGESGRRKTLMFNGYADQVASLYSGMDLDVYF"
     gene            2352..4004
                     /locus_tag="VFV99_20205"
     CDS             2352..4004
                     /locus_tag="VFV99_20205"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5061706.1"
                     /translation="MSTSRSPFVDGRFLKQFVIACCLVPGALLLWDAYQGQLGVNEVN
                     FAIRTTGLVGLVFLTLSLAITPLRRLTKWNVLISARRNFGVFGFFYILTHFVIFWLFD
                     RAGSIGSTLHEIVARPYLWFGFGALLLMTPLAITSFDGMVTRLGAKRWKRLHRLAYAA
                     GIFGVVHYYMLVKADTTQPLAFAVVLGGLLASRFIPTKKPLPKKRMFWSGELELARIV
                     DETHDVKTFRFVNPAGGPLPFEHVAGQYLNLKLTIDGKRVNRSYTIASPPTRRDYCEI
                     SVKKVPNGYGSKHLHETWREGELIKIAAPAGKFYFAGHEAKRIVMIAGGIGITPMMAV
                     ARSLTDRGWTGDIYLVFSIRLVKDIVFRDELAQLVAAHKNLHVCYTVSGDPDTAWDGP
                     RGNITRELLETVVPDLTRGPIMLCGPDAMMTAMRKLLVGMGIPDAEIHQEAFVSPPTP
                     NAGDPEPIDDVPDGIRNLTFERAGKRVQLTNGLTVLEAAEECGVDIPFECRSGICGQC
                     KTRLISGHVAMEVQDALTPNDKMKGLILACQAVCTKDAVIDA"
     gene            complement(4005..4604)
                     /locus_tag="VFV99_20210"
     CDS             complement(4005..4604)
                     /locus_tag="VFV99_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061707.1"
                     /translation="MSAFRRRTKFAAPVILTVAACSSGKEKPKPEPRNAFQVSWQVTM
                     QNMRCEAHMPGANPPPPGQEIECPPGQTGGMYSTVAQVAAGKCVLVPPNCDDPSCAKI
                     VTPCPLPMGQKLVTKLAYVWVIEKRGDKCHAEEEDHDCPPGVDCNPPAPRTFPCPPGV
                     TEEKPMHFAELPDATCVLVPDGCEDTSCATEKIDCPPMK"
     gene            4655..5872
                     /locus_tag="VFV99_20215"
     CDS             4655..5872
                     /locus_tag="VFV99_20215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061708.1"
                     /translation="MGQLVHIGEVVEGRYKIVRPIADGGMGTVFLAEHWLIKRKVALK
                     ILHAELAQDAVMIRRFLNEAMAAGTLGHPHIVESTDMGFTKNDVPYIVFEYLNGTSLA
                     DEITKIKKLTVPRALSIALQTASALEAAHGAGIIHRDLKSDNIFLAKKGDIIDHVKVL
                     DFGISRFLSASDKTARGGNLLGTPEFMAPEQVTDPDHIDHRIDIYALGVLLHEMIAGA
                     VPFVLEKQNDIESAHALLARIVNEPPPSLAVADAPPGLEELATKLLAKDPRDRYQTMA
                     EVQRAIEQLTGTPAPARARTVSREIADISDKTVSGRSRLTPTSLEREVKQLGKRWSLV
                     GVELVLDLHSRQLTKLAAVVGDVAQIADEMELQPRIAIEYPHLRIVLPNAASVAELVF
                     AARVEQWLRENGW"
     gene            5961..6740
                     /locus_tag="VFV99_20220"
     CDS             5961..6740
                     /locus_tag="VFV99_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061709.1"
                     /translation="MKMRHCLLGLVVATPAFARPGDGVTEEDLIPKSEIRDSVEHGEL
                     MPWSVAARHVRLGMLVNTYGGWDAAKDAPVMTGTLEASLFERLTLRASATNPGMTDVL
                     RPSFGALFELTHEEDSGIDLAVGGDYELEGWNHNPALVTRVAAARTAGLTRLQANAAF
                     GLATNSSSERYGDLRLSGLHPIAEGLYAGLDSRARLDLERSGEEPTGELDWDMQAGPV
                     ATLALGRWAVSATGGVSAWKLRSRDEAKVGAIGAVGVGAVF"
     gene            complement(6737..7855)
                     /locus_tag="VFV99_20225"
     CDS             complement(6737..7855)
                     /locus_tag="VFV99_20225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5061710.1"
                     /translation="MRRPSSEDALARLERDRAVLGAIVQRSPIGVAFGNVDGAFEIVN
                     DRLRTIIGKVTSPPVRFQDLAELVDFRWADGSKVTRHALADLLHQAATAPIEVEMESR
                     KGPKPIWVSIRLAPVRAANQQLLGIVGVVRDVTVEHAIANLRKEIVAVIAHDLRSPIA
                     AIKLSIESAAQDWVPGEAHVRVPAKTLTRVEHSAERLGLMVDELLDASHVELGTLPLE
                     RQIVDLCELIARLVDDLRPSLRGHPVATELPSHRVCASVDRLRIQQVLTNLLENAAKY
                     ARPDTTIHVSLREQDGLVDIAVADEGQGIDPDDVPKLFDRFYQAARARQRRQGLGLGL
                     YITKGLVEAHGGSVSVESVVGQGSTFHVQLRSSACGIA"
     gene            8013..8444
                     /locus_tag="VFV99_20230"
     CDS             8013..8444
                     /locus_tag="VFV99_20230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061711.1"
                     /translation="MQSLQYEVLMAAVLVVDDDQDLADVVGEVLRAQGHEVQCAADGS
                     EGLKLVEQKLPDLLVLDVEMPVLSGPDMANRLLIDDMGRENIPIVLLSGVADLSRATA
                     MVGTPYALRKPTTLSSLLAMVRRALAERTAPSPQLPANLPD"
     gene            complement(8441..10468)
                     /locus_tag="VFV99_20235"
     CDS             complement(8441..10468)
                     /locus_tag="VFV99_20235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEX5061712.1"
                     /translation="MHVRLAALGAVIIGLSNGAAHAQLIQEPVEAQMLATGRNDPMPV
                     VEERLTVTIDGQHATTTLLQVFLNNAGAQTEGRYRLRAGMDAHVEGFAYWNGEQKIVG
                     EVFEKQTARQVYDSVTTRRRDPGLLEQDGEGAFTFKVFPIEAKEKKRVEMRWTKWLDR
                     RTKTVTYHAPVSRADADIVIEIVGAVKNVKSPTHRFHSEKTSNGVRLRSDGARTTGEL
                     ILQYDVDEPDWQPNVYVMPGTGKGDGWFAMSLAAPDAGNNVAPKDVTIVVDRSGSMAG
                     EAMEHARSAASDMIRRLDSHDRVNVISFSDEVDPLFKTPQLLDDETRARAMKFADHLK
                     EGGGTDIALALRTAIASQERGQSRPRVIVFMTDGQSDTKLALDAAGADTGDVRLFTLG
                     LGKDVNRPLLERLANVKRGRFVYVDRASAIENEVGKLAASIAKPLLVDVSVEVDGAQA
                     VRLYPRSIGDLFAEDDMLVTGRLRGSGTAKFTIKGRLGGKQVSFTRSVDLGKAVKRPW
                     VGRLWAQARVQHLLEEISLGKQDAEMTNEVIDLALAYNFVTPYTSFLAIPESELGNMK
                     GTVEAARERKQKILADNPEVAALEGKKSSSFGGRGGQSDDEDDDAPMKAKRDVADAPE
                     PGPPGDYDGEGESTSIGRVRKHGCAGCSTGTPSNAGLLVVVALLVLRRRRR"
     gene            complement(10522..11241)
                     /locus_tag="VFV99_20240"
     CDS             complement(10522..11241)
                     /locus_tag="VFV99_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061713.1"
                     /translation="MTMSQDALYLYAVTARVVDEFTSAAMLFTALDVSNAVKQTIPEA
                     RHREISPLVRDIFERRGMGAYIQTSIKVVAAGKGPTTALLYHLPEQTEDMYDATMRQQ
                     LAVPPQMRQQAPEPDTTSSSTEAHVRVGKDGRGRVPKAIIENAGIKGDRVLVKSYPAV
                     PSLQILAAGNAPAAAPPDPSQSAPDVFFAVTNDGQPLNYEHPTLLHIPRSLLDIFGAA
                     DPQTLNLVAKIDGASVVITRR"
     gene            11278..13263
                     /gene="ligA"
                     /locus_tag="VFV99_20245"
     CDS             11278..13263
                     /gene="ligA"
                     /locus_tag="VFV99_20245"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5061714.1"
                     /translation="MEGTLKKPAARIAELAKLLRKYKDAYYNGTPLVSDAAYDALEDE
                     LRALDPAHPVIASVGAPATKQTAWEKARHAIPMGSLNKAVNEEEFRQWADRCNELGKK
                     QKLKSITDDLFVTEKLDGLSLAVTYDKGKLVDAITRGDGQVGERILSNARRMKGVPAK
                     LKGTQSVTVRGEIILKLSDMKKAFPDAANPRNQASGTSKRLDGQGCEHLTVMFYDLDG
                     IDADDEEKKFEILQDLGLLVPNFKACDLDAALGVHKDYSKTKRAKLDYEIDGLVIRAN
                     DVHVQHMLGELNNRPRAAIAFKFASQAKVTTLVDIVWETGSSGRVSPVAVVKPVVLAG
                     ATVQRASLHNAGMIAELDIGIGDEILVSRRNDVIPYVEEVVTKKGKTAKPPTKCPTCG
                     VGLEKVGEYLACRNKKCQALIEGRIENWIDALGVLEWGDKLINQLVEAKLVSEPADLY
                     KLEPEEIAKLERRGMVIAKKVLDNLRAQLPLTLPKFLAALGIENFGLQTAKAIVAAGF
                     DTLDKVQKATAADLAAIPGVGPSKGKAAFDGIKDRKAEIARLVAVGVDPITKTKAGPL
                     LGKTFCFTGSLSKPRKEFEQLVEDNGGTLLSGVTKELNYLVMADPNSGSSKSQKARQY
                     GTLCIDEAGFFALISTSGKAPKPAGKAKRGSAGATAV"
BASE COUNT         2383 a         4452 c         4324 g         2323 t
ORIGIN      
        1 cgatgctgcg tggaccgcga cgaatccgag tggcgttgcc gtgctgcgaa aacgacgcgc
       61 cggctggcag tgcctcgcgc tcgcgccgag ctacgcgagc ttcgtcgcgc ttgccgacgg
      121 cacaccggtc gattggggcg cacggcctgc cgctgcgatc gcgcctcccg atgctttgct
      181 cgcggcaacg gaacgactcg ctggtgcgcg accggacgtg atcgcgatcg acatgccgct
      241 cgcgaccacg cgcatcgtcg cgcgtcgcgc cgccgacaac gcgatcgcga gcgcgtacgc
      301 gacccgcggg ctcggtgcgc acagcccgaa cgagagcagg cccggcccga tcgcgcacgc
      361 gatgcgacaa ggtttttccg cgcacggcta tgcactcgcg acatcggcaa cacctgcggg
      421 cacgcgcacg gtgatgatcg aagtgtttcc ccacgcggct gcgatcgcgc tcctcggcgc
      481 gagctatcgc gtgccgtaca agctggcgcg cgtcactcag tactggcccg atcgatcacc
      541 accgcagcgg cgccgcacgc tgctcgcgca gtggacgaag ctccgccgcg cgctcgccgg
      601 gcacgtcctc gacgtcgagc tacgcgtacc ggcgaccggg acactcagtg cgctcaagcg
      661 ctacgaagac gcgctcgacg cggtgctctg cgcgtggagc ggcgccgagt atctcgctcg
      721 acgcacgcgc gcgtacggtg acgcgaccgc cgccgtgtgg gcgcactagc gctttgcact
      781 cttcgggttc ggattatcgg ccgggttgat gtagtggatg tcgaacgggc ccggtccgct
      841 gacttgcacg atcgtctcgc ccttcgtctg ggcgccgtgg tgcatcttcg ccggcaggaa
      901 gtgatacgag ccggccgtga gcttgtgcgg ctcgcctgtc atcgtgtcgc ccatgtacat
      961 ctgcaacgtg cccgagatga tcgtcaggtg ctcgtcgttg ctgtgccagt gcgccgggat
     1021 cttgtagcca tcgggtgcct tgatgcgcat cgtgaacatc cccggcttgg acggatcgcc
     1081 gtaaagcacg gcgagcttcg cgcccttcgg cacatccggt ggcggctcgc cccacttgag
     1141 ggcgtcgggc gtgatgaaca ctgccccgcc cttctccatc ttctcctgcg cctgcggctc
     1201 gttcttcgcc ggcttcttct tcggatcgtc ggcacgcgct gtcggcgcga tcaacaacgc
     1261 aacgaccgcc gccctcgtca tccacgttct catggctgtc ctcctgctgc ggactttggc
     1321 ttgatgcgtc gcaagacgca ctgcgtaggc ctacgtaacc ggggggctgt aaccgctcgt
     1381 gcgatgatgc gtaagcacgg ggaagccgat gacgaagagc ctcgagaacg aagttacgcc
     1441 cccggaggtc tacttcaacc gccgggcgat cctgcgtgcg ggtgcgatcg ccgccggcgc
     1501 gctcggcacc gggatcctgt atcgcaagct gaacggcgtc gacctcgtgc agacccagac
     1561 cgccgaggtc agcggcgtcg tgaagccgac cgatccggcg tacgccgttg ccgaagcgaa
     1621 gacgccgtac gagtcgatca ccaactacaa caacttctac gagttcacga cgaacaagga
     1681 cggcgtcgcg aaggcagctg ctggcttcaa gacgaacggc tgggccgtcg aggtcggcgg
     1741 gctggtgcac aagccgaagg tcttcgacat ggacgcgatc cgtcggctca gcgcacccga
     1801 ggagcgcatc tatcgcatgc gctgcgtcga ggcctggtcg atggtcgtgc cgtgggttgg
     1861 ctattcgctg tccaagctgc tcgcggtggt cgagccaatg gcgagcgcga aatacgtcgc
     1921 gatgcagacg ctgctcgacg tctcgcgcat gccgggacaa gcgaacgacg tgctcgactg
     1981 gccttacgtc gagggctacc gcatcgacga ggcgatgcat ccgctcgcgt tgctcacgac
     2041 cggactctac ggccgcgagt tgccgcccca agacggcgcg ccggtacgga tgatcatgcc
     2101 gtggaagtac ggcttcaaag ggatcaagtc gatcgtcaag atcacgctcg tcgacaagca
     2161 gcccccgacg tcgtggaaca agtacgcggc gaacgagtac ggcttctacg caaacgtcaa
     2221 cccgaaccat ccgcacccgc gctggagtca ggcgaccgag cagcggatcg gtgagtcggg
     2281 tcggcgcaag acgctcatgt tcaacggcta cgcggatcag gtcgcgagcc tctatagcgg
     2341 gatggatctc gatgtctact tctagatccc cgttcgtcga cggccgcttc ctcaagcagt
     2401 tcgtgatcgc gtgctgcctc gtgcccggcg cgctcctctt gtgggacgcc taccagggcc
     2461 agctcggcgt caacgaggtc aacttcgcga ttcgcacgac cggcctcgtc ggtctcgtgt
     2521 tcctcacgct ctcgctcgcg atcacgccgc tgcgccggct cacgaagtgg aacgtgctga
     2581 tctcggcgcg ccgcaacttc ggcgtgttcg gcttcttcta catcctcacg cacttcgtga
     2641 ttttctggct gttcgatcgc gcgggcagca tcggtagcac gctgcacgag atcgtggcgc
     2701 ggccgtactt gtggttcggc ttcggcgcgc tcctgctcat gacgccgctc gcgatcacgt
     2761 cgttcgacgg catggtgacg cggctcggcg ccaagcgatg gaagcgactc caccgccttg
     2821 cctacgccgc cggcatcttc ggcgtcgtgc actactacat gctcgtcaaa gcagatacga
     2881 cgcagccact cgcgttcgcg gtcgtgctcg gcggcttgct cgcctcgcgg ttcattccaa
     2941 cgaagaagcc gttaccgaag aagcgtatgt tctggtccgg cgagctcgag ctcgcacgca
     3001 ttgtcgacga gacgcacgac gtcaagacgt tcaggttcgt gaaccctgcg ggcggtccgt
     3061 tgccgttcga gcacgtcgcc ggccagtacc tgaacctcaa gctcacgatc gacggcaagc
     3121 gagtcaaccg ctcgtacacg atcgcgtcgc caccgacgcg gcgtgactac tgcgagatct
     3181 cggtgaagaa ggtgccgaac ggttacggct cgaagcacct gcacgagacg tggcgggaag
     3241 gtgagctcat caagatcgcg gcgccagcgg gcaagttcta cttcgcaggc cacgaggcaa
     3301 agcgcatcgt gatgatcgcg ggcggcatcg gcatcacgcc catgatggcg gtcgcacgca
     3361 gcctcaccga tcgcggctgg accggcgaca tctacttggt gttctcgatc cggctcgtga
     3421 aagacatcgt gtttcgcgac gagctcgcgc aactggtcgc tgcacacaag aaccttcacg
     3481 tttgctacac ggtgtccggc gatcccgaca ccgcgtggga cgggccgcgt ggaaacatca
     3541 cgcgcgagct gctcgagacg gtcgtgccgg acctgacgcg cggtccgatc atgctgtgcg
     3601 gtcccgacgc gatgatgacg gcgatgcgca agctgctcgt cggtatgggc atccccgatg
     3661 ccgagatcca tcaagaggcg ttcgtatcgc caccgacgcc gaacgccggc gatccagaac
     3721 cgatcgacga cgttcccgac gggatccgca acctgacgtt cgagcgcgcc ggcaagcgcg
     3781 tgcagctgac gaacgggctc accgtactcg aagccgccga ggaatgcggc gtcgacatcc
     3841 cgttcgagtg tcggtccggc atctgcggac agtgcaagac gcgtctcatc tcgggacacg
     3901 tcgcgatgga agtgcaggac gcgctgacgc cgaacgataa gatgaagggc ctcatcctcg
     3961 cgtgccaggc cgtgtgcacg aaggacgcgg tcatcgacgc ttagctattt catcggcggg
     4021 cagtcgatct tctcggtcgc acagctcgtg tcttcgcagc catctggaac gagcacgcag
     4081 gtcgcgtcgg gcagctcggc gaagtgcatc ggcttctcct cggtgacccc gggcggacac
     4141 gggaacgtgc gcggcgcagg aggattgcag tcgacgccgg gcgggcaatc gtggtcctcc
     4201 tcctcagcgt ggcacttgtc gccgcgcttc tcgatcaccc agacgtacgc cagcttcgtc
     4261 acgagcttct gccccatcgg caacgggcac ggcgtcacga tcttcgcgca tgacgggtcg
     4321 tcgcaattgg gtggcacgag cacgcacttg ccggccgcga cctgcgcgac ggtcgagtac
     4381 atgccaccgg tctgtcccgg cgggcactcg atctcctgac ccggcggtgg cggattcgca
     4441 ccgggcatgt gcgcctcgca ccgcatgttc tgcatcgtca cctgccacga cacctggaac
     4501 gcattacgtg gctccggttt gggtttctct ttcccactcg agcatgcggc gacggtcagg
     4561 atcacgggag ccgcaaactt cgtacggcgg cggaacgcgc tcatgcctca tcctaatgcg
     4621 ctctgccctg acttcgccgt actatcggcg ggcgttgggc cagcttgttc acatcggcga
     4681 ggttgtcgaa ggccgataca agatcgtgcg tccgatcgcc gacggtggca tgggcaccgt
     4741 cttcctcgcc gagcactggc tgatcaagcg caaggtcgcg ctcaagatcc tgcatgccga
     4801 gctcgcacag gacgccgtca tgatccggcg tttcctcaac gaagcaatgg ccgccggcac
     4861 gctcggtcat ccccacatcg tcgagtcgac ggacatgggg ttcacgaaga acgacgtgcc
     4921 gtacatcgtg ttcgagtacc tgaacggcac gtcactcgcc gacgagatca cgaagatcaa
     4981 gaagctgacc gtaccgcgcg cactctcgat cgcgttgcag acggcgtccg cgctcgaagc
     5041 ggcccacggc gcgggcatca ttcatcgcga tctcaagtcc gacaacatct tcctcgcgaa
     5101 gaagggcgac atcatcgacc acgtcaaggt gctcgacttc ggcatctcgc ggttcctcag
     5161 cgcatcggac aagaccgcgc gcggcggcaa cctactcggc acgccggagt tcatggcgcc
     5221 cgagcaagtc accgatccgg accacatcga tcatcgcatc gatatctacg cgctcggcgt
     5281 gctccttcac gagatgatcg caggcgcagt gccgttcgtg ctcgagaagc agaacgacat
     5341 cgaatccgcg cacgcgctcc tcgcgcgcat tgtcaacgag ccgccgccat cactcgccgt
     5401 tgccgatgcg ccgccgggtc tcgaagagct cgcgacgaag ctgctcgcga aggacccacg
     5461 cgatcgctac cagacgatgg cggaggtaca gcgcgcgatc gaacagctga cgggaacacc
     5521 ggcgccggcg cgcgcgcgca ccgtgtcgcg cgagattgcc gacatctcgg acaagaccgt
     5581 cagcggccgc tcgcgactga caccgacgtc actggagcgc gaggtcaagc aactcggcaa
     5641 gcgctggtcg cttgtcggcg tagagctcgt gctcgatctg cacagccgtc agctgacgaa
     5701 gctcgctgca gtcgtcggtg atgtcgccca gatcgccgac gagatggagc tgcagccgcg
     5761 catcgcgatc gagtatccgc acctacgcat cgtattgccg aatgcggcca gcgtcgccga
     5821 gctcgtgttc gctgcgcgcg tcgaacagtg gctgcgcgaa aacggctggt gagcgtcggc
     5881 gcgcagtgcg cccccgcgca gccgtagata cgagctgcta cgtacgtcgg cacgcggctc
     5941 gcacgtaatc tcacgtagcc atgaagatgc ggcattgctt gcttggcctg gtcgtcgcca
     6001 cgcccgcgtt cgcgcggccc ggcgatggcg tcacagaaga agacctgatt ccgaagagcg
     6061 agatccgcga cagcgtggag cacggcgagc tgatgccgtg gtcggtcgct gcgcgtcacg
     6121 tccgcctcgg catgctcgtc aacacctacg gcggctggga cgccgcgaag gacgcaccgg
     6181 tgatgaccgg cacgctggag gcaagcttgt tcgagcggct gacgctgcgc gcatcggcca
     6241 cgaatccggg catgacggac gtgctgcggc cgagcttcgg cgcactcttc gagctcactc
     6301 acgaggagga ctcgggcatc gacctcgcag tcggcggtga ctacgagctc gaaggctgga
     6361 atcacaatcc agcgctggtc acgcgtgtcg cagcagcgcg aaccgccggt ctcacacgcc
     6421 tccaggcgaa cgcagcgttc ggcctggcga caaacagcag cagcgagcgc tacggtgatc
     6481 tgcgcctctc gggcctgcat ccgatcgccg agggcctcta cgccggcctc gactcgcgcg
     6541 cgcgactcga tctcgagcgt agtggcgagg agccgaccgg agagctcgac tgggatatgc
     6601 aggccggtcc agtggcgacg ctcgcactcg gtcgctgggc ggtgagtgcg accggcggtg
     6661 tctcggcgtg gaagctgcgc tcgcgcgacg aggcgaaggt cggcgcgatc ggtgcggttg
     6721 gcgtcggcgc cgtgttctag gcgatgccgc aggcactgct gcgaagctgg acgtggaacg
     6781 tgctgccctg accgacaacg ctctcgaccg acacgctgcc accatgcgct tcgacgagcc
     6841 ctttcgtgat gtagagtccc agcccgaggc cctgtctacg ctgccgcgcg cgcgccgcct
     6901 ggtagaaccg gtcgaagagc ttaggcacgt cgtcaggatc gatcccctgc ccttcatccg
     6961 cgacggcgat atcgaccagg ccatcttgct cgcgcagtga gacatgaatc gtcgtgtctg
     7021 ggcgtgcgta cttcgccgcg ttctcgagca ggttcgtgag cacctgctgg atgcgcaaac
     7081 gatcgaccga cgcgcacacg cgatgcgacg gcagctcggt cgcgactggg tgaccgcgca
     7141 agctcggtcg caagtcgtcg acgagcctgg cgatgagctc gcagagatcg acaatctgac
     7201 gctcgagcgg cagtgtgccg agctcgacgt gcgaggcatc cagcaactcg tccaccatga
     7261 gcccgaggcg ctcggcgcta tgctcgacgc gagtgagcgt cttcgcgggg acacgaacgt
     7321 gcgcttcgcc gggaacccaa tcttgcgctg cggactcgat cgacaacttg atcgccgcga
     7381 tcgggctccg cagatcatgg gcgatcaccg cgacgatctc cttgcgcagg tttgcgatcg
     7441 cgtgctcgac ggtcacatcg cggacaacgc cgacgattcc gagcagctgc tggttcgcag
     7501 ctcgaacggg cgctagccgg atcgacaccc agattggctt gggacccttg cgtgattcca
     7561 tctccacctc gatcggtgca gtcgccgcct gatgcagcag atcggccaac gcgtgccgcg
     7621 tgaccttgct tccgtcggcc cagcgaaagt ctacgagctc tgcgagatcc tggaagcgca
     7681 ccggcggcga cgtgaccttg ccgatgatcg ttctgagccg atcgttgacg atttcgaacg
     7741 cgccgtcgac gttaccgaag gcgacgccga tcgggctgcg ctgcacgatc gccccgagca
     7801 ccgcgcgatc gcgttcgagt cgtgcgagcg catcctcgct gcttggccga cgcaccggcg
     7861 cgtaccgatc gtgcactggc ttcagcgtta ctcggcgaat caccgtgctc gccaagagcg
     7921 cgagcgaacc gaaactcaga acgatcaggc tacgcgggct cacgagaggg caagaagcaa
     7981 ctcacatgcc cccggccacc gcggcatcac cgatgcaatc gctgcagtac gaggttctca
     8041 tggctgccgt acttgtcgtc gatgacgacc aagatctagc cgacgtcgtc ggggaggtcc
     8101 tgcgtgcaca gggacatgag gttcaatgcg ccgctgatgg ctccgaggga ctaaagctcg
     8161 tcgagcagaa gcttcccgat ctgctcgtgc ttgacgtcga aatgccggtc ctctcggggc
     8221 ctgatatggc caatcggttg ctgatcgacg atatgggacg cgaaaacata ccgatcgtgt
     8281 tgctcagtgg cgtcgcggac ctgtcgcgcg cgactgccat ggtgggcacg ccgtacgcac
     8341 tacgaaaacc gaccacgtta tcgagcctgc tcgcaatggt gcgccgagcc ctggccgaac
     8401 ggactgcgcc atcaccgcag cttcccgcga acctgcccga ctaacggcgg cgccgacgca
     8461 acacgagtag cgcgacgacc acgaggaggc ctgcattgct cggggtacct gtcgagcagc
     8521 cggcacagcc gtgcttgcgc acgcggccga tgctcgtgct ctcgccctcg ccgtcgtagt
     8581 caccaggcgg gcctggctcg ggggcgtcgg cgacgtctcg tttcgctttc atcggggcgt
     8641 catcgtcttc gtcatcgctc tggccaccac ggccgccgaa gctcgacgac ttcttgcctt
     8701 cgagcgcggc cacctctgga ttgtcggcga ggatcttctg cttgcgctcg cgtgcggcct
     8761 cgaccgttcc tttcatgttg ccgagctcgc tctccggaat cgcgaggaac gacgtgtacg
     8821 gtgtgacgaa gttgtacgcg agcgcgagat cgatcacctc gttcgtcatc tcggcgtcct
     8881 gcttgccgag cgagatttct tcgagcagat gctgcacgcg cgcctgtgcc cacaaccgac
     8941 cgacccacgg ccgcttgacc gccttgccga gatcgaccga gcgcgtgaac gagacctgct
     9001 tgccgccgag ccggcccttg atcgtgaact tcgcggtacc ggagccacgc aggcggccgg
     9061 tcacgagcat gtcgtcctcg gcgaacaggt cgccgatcga acgcgggtag aggcgaaccg
     9121 cttgcgcacc atcgacctcg acggagacgt cgacgagcag cggcttcgcg atgctcgcgg
     9181 ccagcttgcc gacttcgttc tcgatcgccg acgcgcggtc gacatagacg aagcggccgc
     9241 gcttgacgtt cgcgaggcgt tcgagcaacg gacgattgac gtccttgccg aggccgagcg
     9301 tgaacagccg cacgtccccc gtgtcggcgc ctgccgcgtc gagtgcgagc ttcgtgtcgg
     9361 actggccgtc ggtcatgaac acgatcacgc gcggtcggct ctggccgcgc tcctgcgaag
     9421 cgattgccgt gcgcagcgcg agtgcgatat cggtgccgcc gccttctttg aggtgatcgg
     9481 cgaacttcat cgcgcgggcg cgggtttcgt cgtcgagcag ctgcggcgtc ttgaacagcg
     9541 gatcgacctc atcggagaac gagatcacgt tgacgcgatc gtgcgaatcg agtcggcgga
     9601 tcatgtcaga cgcagccgag cgcgcgtgct ccatcgcttc accggccata ctgcccgagc
     9661 gatcgacgac gatcgtgacg tccttcggcg cgacgttgtt gccagcgtcc ggtgcggcga
     9721 gcgacatcgc aaaccaaccg tcgcccttgc cggtgcccgg catcacgtac acgttcggct
     9781 gccagtccgg ctcgtcgacg tcgtactgca ggatgagctc gccagtcgtg cgcgcaccgt
     9841 cggagcgcag ccgcacgccg ttgctggtct tctcgctatg gaagcgatgc gtcggcgact
     9901 tcacgttctt caccgcaccg acgatctcga tgacgatgtc cgcatcggcg cgcgaaaccg
     9961 gcgcgtgata ggtgaccgtc ttcgtgcggc ggtcgagcca cttcgtccac cgcatctcga
    10021 cgcgcttctt ctctttcgct tcgatcggga acaccttgaa tgtgaacgct ccctcgccat
    10081 cctgctcgag caagcccgga tcgcgacggc gcgtcgtcac ggagtcgtag acctggcgcg
    10141 cggtctgttt ctcgaatacc tcgccgacga tcttctgctc gccgttccaa taggcaaagc
    10201 cctcgacgtg ggcatccatg ccggcgcgta agcgatagcg cccttcggtc tgtgcaccgg
    10261 cgttgttcag gaacacctgt aacaacgtcg tggtcgcgtg ttggccgtcg atcgtgacgg
    10321 tcaaccgttc ctcgacgacc ggcatcggat cgttgcgacc cgtggccagc atctgtgctt
    10381 cgacaggctc ctggatcagc tgcgcatgcg cagccccatt cgacagcccg atgatgaccg
    10441 ctcccaatgc agctagacgg acgtgcatgg gcctgccaac gcatgcaccc aaggaccggt
    10501 ctaattttgg tgtcgcgccg gctaacggcg ggtgatcacg acggacgcgc catcgatttt
    10561 tgccaccagg ttcagcgtct gcgggtccgc agcgccgaag atatcgagca gcgagcgcgg
    10621 aatgtggagc agcgtcgggt gctcgtagtt gagcggctgg ccatcgttgg tcacggcgaa
    10681 gaacacatcg ggcgcggact ggctcggatc aggcggcgcc gccgcgggcg cattgccggc
    10741 cgcaagaatc tgcaggctcg gcaccgccgg atacgacttc acgagcacgc ggtcaccttt
    10801 gatgcccgcg ttttcgatga ttgctttcgg cacgcgacca cgtccgtcct tgccaacacg
    10861 cacgtgcgcc tccgtcgacg atgacgtcgt gtcgggctcg ggcgcttgct gccgcatctg
    10921 cggcggaacc gcgagctgct ggcgcatcgt ggcgtcgtac atatcttccg tctgctccgg
    10981 caaatgatag agcagcgccg tcgtcggacc tttgcccgcc gcgacgacct tgatcgacgt
    11041 ctggatgtac gcgcccatgc cgcgtcgctc gaagatgtcg cgcacgagcg gtgagatttc
    11101 gcgatggcgc gcctcgggaa tcgtctgctt cacagcattc gatacgtcga gcgctgtgaa
    11161 cagcatcgcg gcactcgtga actcgtccac cacacgtgcc gtcacggcat acaggtacag
    11221 cgcgtcttga ctcatcgtca tcccccagga gatcgtcgtc gacgattcta cgcaaggatg
    11281 gaggggacct tgaagaagcc ggccgctcga attgctgagc tcgcgaagtt actccggaag
    11341 tacaaggacg cgtactacaa cggcaccccg ctcgtcagcg acgctgccta cgacgccctc
    11401 gaggacgagc ttcgcgcgct cgacccggca catccggtca tcgcgagcgt cggcgcgccg
    11461 gcgacgaagc agaccgcctg ggagaaagcg cgccacgcga ttccgatggg gtcgctgaac
    11521 aaagcggtca acgaggaaga gtttcgccag tgggccgacc gctgcaacga gctcggcaag
    11581 aagcagaagc tcaagtcgat caccgacgat ctgttcgtga ccgagaagct cgatggcctg
    11641 tcgctcgccg tcacctatga caagggcaag ctcgtcgacg cgatcacgcg cggcgatggc
    11701 caggtcggcg aacgcatcct gtcgaacgcc cgccgcatga agggggtgcc ggccaagctc
    11761 aagggcacgc aatcggtcac cgtccgcggc gagatcatcc tcaagctctc ggacatgaag
    11821 aaggcgttcc ccgatgcagc gaacccgcgg aaccaggcgt cgggcaccag caagcgcctc
    11881 gacggccaag gctgcgagca cctgacggtc atgttctacg acctcgacgg catcgatgcc
    11941 gacgacgagg aaaagaagtt cgagatcctg caggacctcg gcctcctggt accgaacttc
    12001 aaagcgtgcg acctcgacgc tgcgctcggc gtccacaagg actacagcaa gacgaagcgc
    12061 gcgaagctcg actacgagat cgacggcctc gtgatccgcg cgaacgacgt gcacgtgcaa
    12121 cacatgctcg gcgagctcaa caaccggcca cgggccgcga tcgcgttcaa gttcgcgtca
    12181 caggcgaagg tgacgacgct cgtcgacatc gtgtgggaaa ccggctcgtc cggccgcgtg
    12241 tcgccggtcg cagtcgtgaa gccggtggtg ctggctggcg ccaccgtgca gcgcgcgtcg
    12301 ctgcacaacg cgggaatgat cgccgagctc gacatcggca tcggcgacga gatcctggtg
    12361 tcgcgccgca acgacgtcat tccgtacgtc gaggaagtcg tcacgaagaa aggcaagacc
    12421 gcgaagccgc cgaccaagtg cccgacgtgc ggtgtcggcc tcgagaaggt cggcgagtac
    12481 ctcgcgtgcc gcaacaagaa gtgccaggcg ctgatcgaag gtcgcatcga gaactggatc
    12541 gacgcgctcg gcgtgctcga gtggggcgac aagctgatca accagctcgt cgaggcaaag
    12601 ctcgtcagcg agcccgccga cctctacaag ctcgagcccg aagagatcgc gaagctcgag
    12661 cgccgcggca tggtgatcgc gaaaaaagtc ctcgataacc tgcgcgcgca gctgccgctg
    12721 acgctaccga agttcctcgc cgcgctcggc atcgaaaact tcggcctcca gaccgcgaaa
    12781 gcgatcgtcg ccgccggctt cgacacgctc gacaaggtgc agaaagcgac cgccgccgac
    12841 ctcgcggcga ttccgggcgt cggcccgagc aagggcaaag cggcgttcga tggcatcaag
    12901 gaccgcaagg ccgaaatcgc gcgcctcgtc gccgtcggcg tcgatccgat cacgaagacg
    12961 aaggccggcc cgctgctcgg caaaacattc tgcttcaccg gttcgctgtc gaagccgcgc
    13021 aaggaattcg agcagctcgt cgaggacaac ggcggcacgc tgctgtcggg cgtcacgaag
    13081 gagctcaact acctcgtgat ggccgatccg aattcggggt cgagcaaatc gcagaaggcg
    13141 cggcagtacg ggacgctgtg catcgacgag gccggcttct tcgcgttgat cagcacgagc
    13201 ggcaaggctc cgaagcccgc cggcaaagcg aagcgcggca gcgccggcgc gaccgccgtg
    13261 tgaatccgcc gggttgatcc atcgccgatg gccgggttgc gcgtcagcaa ccgagccatt
    13321 gggtggccga aagcggcctt cagatggaac gactatgtag tggcggtcgc gtcaagcgca
    13381 gacctgtccc gtccggggct tgaggtcccg gcacatcgtt cgtgttgatc ggcccggtgg
    13441 tcggctgtcc aggaaggtgg atgtcgggtt gtcgcagtcg ac
//
LOCUS       DASUFX010000505         5684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1609977, whole genome shotgun sequence.
ACCESSION   DASUFX010000505 DASUFX010000000
VERSION     DASUFX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609977"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFV99_20250"
     CDS             <1..726
                     /locus_tag="VFV99_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061715.1"
                     /translation="LVVVLGGAGKPAAAACRLAPCPPWVNAIGYTFAGAAIGGYTYGT
                     GYLIYHDMTDPDQSLNYGVAEFTTNGLGTALFGGVMIDALAHDHAGTAATVAPFAILH
                     SALMVHGGWRMYREREDFRAPQNAMLWLGGTAFVTNALVWSSQLGERHGRGFGVAEAA
                     VNAPLAAGIGYLAYDRLNNWRPSGFLYGGMAAISAGFAIHGLRKVIAPSPPLIDVHGL
                     DLSPTVVSDGIEMAPGLGTVRTW"
     gene            736..1236
                     /locus_tag="VFV99_20255"
     CDS             736..1236
                     /locus_tag="VFV99_20255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5061716.1"
                     /translation="MAERRHPNVINTDEVEVTEQKKGTFHARNRRLGPHAGNAQLGAT
                     LTELPPGTMSYPFHYHCANEEAIFIVSGTGTARIGDARVAVRAGDWIALPVGPEHAHQ
                     MINDGEEPLRYLCVSTKHICEVVGYPDSKKVAAWAGPVPTKPWIRQIVREGETLDYWD
                     SEPGAQ"
     gene            1272..2588
                     /locus_tag="VFV99_20260"
     CDS             1272..2588
                     /locus_tag="VFV99_20260"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5061717.1"
                     /translation="MLGRHDAIEAVHVQVGEHELRGNLGVPQGPVGLVIFAGTLDHYV
                     AEALRARGLATLVVGLELDDSEHYDIDLLAMRLIAVTDWIVEHSSLSHLPIGYFGAGT
                     GAASALVAASQRPDVVRAIALSDGRADLAGSALPLVRAPTLLVGPGSHENALEVDRAA
                     IEQMTTVTQFVIIPAASPRFEQPGEIDEVVQLAAEWFVEHFSHVLVEPANKRGSWGRQ
                     FRDRSAAGERLAQLLAKYAHEDVVVFGLPRGGVPVADEIAKALDAPLDVWLVRKIGMP
                     IQPELGMGALAEGAALVLDPKIVRWSGATPDDLKALVHRKVTEIRRRAQRYRGEAPAP
                     DIHGRTIILVDDGIATGGTLRAAIRGARKRGAARVIVAAPVAAAEAAASLKLEADELV
                     CLATPRRLNAVGAWYQDFRQLPEQDVIDILTLARDRFAHSKLRAGA"
     gene            2667..3017
                     /locus_tag="VFV99_20265"
     CDS             2667..3017
                     /locus_tag="VFV99_20265"
                     /inference="COORDINATES: protein motif:HMM:NF045308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5061718.1"
                     /translation="MVALSIQAVARDRLLARRAQLLRRFRYASELADELAEDHNVEII
                     DRANDQWDAHVLGKLGDTETKQLSAVIAALRRIAAGTYGTCVRCQHKIPKGRLDALPE
                     AALCADCARYVHAT"
     gene            complement(3014..3559)
                     /locus_tag="VFV99_20270"
     CDS             complement(3014..3559)
                     /locus_tag="VFV99_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061719.1"
                     /translation="MEGPKQDAPPPVVVSLDGSEQDARIGRARKWLVAIAAIFLVSGF
                     VFYAIQSEDAEKVIRESEQNLSMLDAEQRDQAVMAEVNMTWDQWVSHTRNEVTMNLFL
                     NIGLALSFVGLFFYAKKNPLAATVMALLLYLSVIAYNAILDPKTLPQGFIIKILFIGA
                     LVKAITAAKEERAKMPPAKLR"
     gene            complement(3577..4608)
                     /locus_tag="VFV99_20275"
     CDS             complement(3577..4608)
                     /locus_tag="VFV99_20275"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061720.1"
                     /translation="MLRPEVRANDFLVAESKAMRAVAQQVEEYADGDKPVLICGEHGT
                     GRELVARVLHQRGPRGTGRFVAVRPTFEGSDVPCGAGDDACERARRALRAAHGGTLLV
                     KDVSDLSAPSQRTLKRAIRDRKEPEAADVHVVATADIDVDHAVDARIVSRDFYDLFAP
                     RRIEVPPLRERIEDLPTLFERWIKHYSAEVGRTKPTVSSRAHERLTAYPWPGNVAELK
                     SIARRLLVHVSRSRIEAGDVDEVLPKVAARVPLEDLAFEEMVKSKLAGLLARIDGYPV
                     HDLYEKVLARVERPLFDLVLAHTGGNQLKAAEILGLNRNTLRKKLADLGMESKKKRTG
                     REDRALDEA"
     gene            complement(4612..>5684)
                     /gene="dusB"
                     /locus_tag="VFV99_20280"
     CDS             complement(4612..>5684)
                     /gene="dusB"
                     /locus_tag="VFV99_20280"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX5061721.1"
                     /translation="VVRIGPIHIDTPVLLAPMAAVTDLPFRTVAEELGVGFTITEFLS
                     AHALAVGDKKTSAKLTASLDGRQFGVQIFGREPDKMDAAAKMAVAIGASLVDINMGCP
                     AKRVVAGECGSALMREPAIAQELVRTVVAAVPAHVPVTVKHRAGWNQDNRNAPEFACA
                     MVEAGAQMITVHGRTRTQGFSGKSDNEIIRRVRDALPAHIPLVGNGDVVDVAGFLRMR
                     EETGCDAVMIGRGALGNPWLFSRLREVCAGRPDPGAPTLEERVHVFRRHIDLIEKLKN
                     GPRTLHEVRKACAWYARGLYGCNALRLRVWEAPDLATARALVDDYFTQLIDRQRRLGL
                     SPDGERATMDASFETESPEEAA"
BASE COUNT          933 a         1931 c         1867 g          953 t
ORIGIN      
        1 ctcgtggtcg tgctcggggg cgccggcaag cctgctgccg cagcctgccg cctcgctccg
       61 tgtccgccgt gggtcaacgc gatcggctac acgtttgcgg gtgcagcgat cggcggctac
      121 acgtacggca ccgggtacct catctatcac gacatgacgg atcccgatca gtcgctgaac
      181 tacggcgtcg ccgaattcac gacgaatggg ctcggcaccg cactgttcgg cggcgtcatg
      241 atcgatgcgc tcgcgcacga ccacgccggc accgcagcga cggtcgcgcc gttcgcgatc
      301 ttgcattcgg cgctgatggt gcacggaggc tggcgcatgt atcgcgaacg cgaggatttc
      361 cgcgcgccgc agaacgcgat gctatggctc ggcggcaccg cgttcgtgac gaatgcattg
      421 gtgtggagct cgcagctcgg cgagcgacac ggtcgcgggt ttggcgtcgc cgaggcggcc
      481 gtcaacgccc cgctcgcagc cggcatcggc tatctcgcat acgatcggct gaacaattgg
      541 cggccgagcg gcttcctcta cggcggcatg gcggcgatct cggcggggtt tgcgattcac
      601 ggcttgcgca aggtgatcgc gccgtcgccg ccgctgatcg acgtccacgg cctcgacctc
      661 tcgccgacag tcgtctccga cgggatcgaa atggcacccg ggctcggcac cgtccgcacg
      721 tggtaatcac gacgcatggc cgaacgtcga cacccgaacg tcatcaacac cgacgaagtc
      781 gaggtcaccg aacagaagaa gggcacgttc cacgcgcgta atcgccgact cggcccgcac
      841 gctggtaacg ctcagctcgg tgcaacgctg accgagctgc cgcccggcac gatgtcctat
      901 ccgttccact atcactgcgc gaacgaggaa gcgatcttca tcgtgagcgg cactggtacc
      961 gcgcggatcg gtgatgcgcg cgtcgcggtg cgcgcgggtg attggatcgc gctgccggtc
     1021 ggtcccgaac acgcgcatca gatgatcaat gatggcgagg agccgctgcg ctacctgtgc
     1081 gtgtcgacga agcacatatg cgaagtcgtg ggctatcctg attcgaagaa agtcgcggcg
     1141 tgggctgggc cggtgccgac gaagccgtgg attcgccaga tcgtgcgcga aggcgagacg
     1201 ctcgattatt gggatagcga accgggcgca cagtaatcgc cgctggcccg gccgctgcat
     1261 tgcagacggg catgctcggg cgtcacgacg cgatcgaagc ggttcacgtc caggtcggcg
     1321 aacacgagct ccggggcaac ctcggtgtgc cccagggccc ggttggcctc gtgatctttg
     1381 cgggcacgct cgaccactat gtagcagagg cgctccgtgc acgcgggctc gctacgttag
     1441 tcgtcggcct cgagctcgac gatagcgagc actacgatat cgacctgctc gcgatgcgtc
     1501 tcatcgcggt caccgattgg atcgtcgagc actcgagcct gtcgcatctg ccgatcggtt
     1561 acttcggtgc gggcaccggc gccgcgtcgg cgcttgtcgc agcatcgcag cgccccgacg
     1621 tcgtgcgtgc aatcgcgctc tccgatggcc gcgccgatct cgcgggctcg gcgttgccac
     1681 tcgtgcgtgc accgacgttg ctcgtcgggc cgggcagtca cgagaacgcg ctcgaggtcg
     1741 atcgcgccgc gatcgagcag atgacgacag tcacgcagtt cgtgatcatt ccggccgcga
     1801 gcccgcgctt cgagcagccc ggcgagatcg acgaggtcgt acagctggcg gcggaatggt
     1861 tcgtcgagca cttctcacac gtgctcgtcg agcccgcgaa caagcgaggt agctggggcc
     1921 gccagttccg cgatcggagc gccgctggcg aacgcctcgc gcagctgctc gcgaagtatg
     1981 cccacgagga cgtcgtcgtc ttcggcttgc cgcgtggggg cgtacctgtc gccgacgaga
     2041 tcgcgaaggc gctcgacgcg ccgctcgacg tctggctcgt ccgcaagatc ggcatgccga
     2101 tccagcccga gctcggcatg ggcgccctcg ccgagggggc cgcactcgtg ctcgatccga
     2161 agatcgtgcg gtggtcgggt gcgacgccag acgacctcaa ggcgctcgtt caccgcaagg
     2221 ttaccgagat ccgtcgtcgt gctcagcgct atcgcggaga ggcacccgca cccgacattc
     2281 acggacggac gatcatcctc gtcgacgatg gcatcgccac cggcggaacg ctacgtgccg
     2341 cgatccgtgg cgcacgcaaa cgcggcgcgg cacgcgtcat cgttgcggca ccggtcgccg
     2401 cggcggaggc ggccgcaagc ctgaagctcg aagccgacga gctggtctgt ctcgcaaccc
     2461 cgaggcgcct caacgcggtt ggcgcctggt accaggactt ccgtcagctg ccggaacagg
     2521 acgtcatcga catcctcacc ctcgcccgcg atcgcttcgc ccacagcaag ctgcgcgcgg
     2581 gcgcctgatg ccagcgcaac cgctgcgcgc tcgccgccgg gaaacagccg gttgcgctgg
     2641 cgcacgtgat gcagttacgc cgcgtcatgg tggccttgtc gatccaagca gtggcacgcg
     2701 atcgcttgct cgcacggcgc gcgcagctgc tccgacgttt ccgatacgcg agtgagctgg
     2761 cggacgagtt ggcggaggat cacaacgtcg agatcatcga ccgcgccaac gaccagtggg
     2821 acgcgcacgt actgggcaag ctcggcgaca ccgagacgaa gcagttatcg gcggtgatcg
     2881 ccgcgctgcg ccggatcgcc gcaggcacgt acggcacctg cgtccgctgc cagcacaaga
     2941 tcccgaaggg ccgcctcgac gcgctgcccg aagcagcgct gtgcgccgac tgcgcgcgct
     3001 acgtccacgc gacctagcgc agcttcgccg gtggcatctt ggcgcgctct tccttcgccg
     3061 ccgtgatcgc cttcacgagc gcacctatga agaggatctt gatgatgaag ccctgcggta
     3121 atgtcttcgg atcgaggatc gcgttgtaag cgatcacgct caggtagagc agcaacgcca
     3181 tcaccgtcgc cgcgagcgga ttcttcttcg cgtagaagaa gaggcccacg aacgacagcg
     3241 cgaggccgat gttcaagaag aggttcatcg tgacctcgtt gcgggtgtgc gaaacccact
     3301 ggtcccaggt catgttgacc tcggccatca ccgcttggtc gcgctgttcc gcgtcgagca
     3361 tcgagagatt ctgctcgctc tcgcggatca ccttctcggc gtcttcgctc tggatcgcgt
     3421 agaagacgaa cccgctgacg aggaagatgg ctgcgatcgc gacgagccac ttgcgcgcgc
     3481 gaccgatgcg cgcgtcctgc tccgaaccgt cgagcgacac caccaccggc ggcggcgcgt
     3541 cctgcttggg gccttccatc ccccgatgat acgcaactac gcttcgtcga gggcgcggtc
     3601 ctcgcgaccg gtgcgcttct ttttcgattc catgccgaga tcagcgagct tcttacgcag
     3661 cgtgttgcgg ttgaggccca ggatctcggc cgctttgagt tgattgccgc ccgtgtgggc
     3721 gagcacgaga tcgaacagcg gccgctcgac gcgtgcgagc accttctcgt acaggtcgtg
     3781 caccgggtat ccatcgatgc gcgcgagtag gccggcgagc ttcgacttca ccatctcctc
     3841 gaacgcgaga tcctcgagcg gcacgcgcgc ggcgactttc ggcagcacct cgtcgacgtc
     3901 gccggcctcg atgcggctgc gcgacacgtg gacgagcaag cgccgcgcga tcgacttgag
     3961 ctcggcgacg ttgccgggcc acggatacgc ggtgagccgt tcgtgcgcgc gactcgacac
     4021 ggtcggcttc gtgcggccga cctccgcgga gtagtgcttg atccagcgct cgaacagcgt
     4081 gggcagatcc tcgatgcgct cgcgcagcgg cggcacttcg atccggcgcg gcgcgaacag
     4141 atcgtagaag tcgcgcgaca cgatccgggc gtcgaccgcg tgatcgacat cgatgtctgc
     4201 ggtcgcaacg acgtgaacgt cggcagcctc cggctccttg cgatcgcgaa tcgctcgctt
     4261 cagcgtgcgt tgcgacggcg ccgacagatc gctgacgtct ttgacgagca gcgtgccgcc
     4321 gtgtgccgca cgcaacgcgc ggcgcgcgcg ctcgcaggcg tcatcaccgg cgccgcacgg
     4381 gacgtcgctg ccctcgaacg tcggccgaac cgcgacgaag cggccggtac cgcgtggtcc
     4441 gcgctgatgc agcacgcgcg cgacgagctc gcgccccgtg ccgtgctcgc cgcagatcag
     4501 aaccggcttg tcgccgtccg cgtactcttc gacttgctga gccaccgcgc gcatcgcctt
     4561 gctttcggcg acgagaaaat cgttcgcgcg cacttcgggt cggagcatcg tttacgccgc
     4621 ctcttccgga gattccgtct cgaacgaagc gtccatcgtc gcgcgttcgc catcgggcga
     4681 caagcccaaa cggcgctgac gatcgatcag ctgcgtgaag tagtcgtcga cgagcgcgcg
     4741 cgccgtcgcg aggtccggag cttcccacac gcgcaagcgc agggcgttgc atccgtacag
     4801 accgcgcgcg taccacgcgc acgctttgcg aacttcatgc aaagtgcgcg gcccgttttt
     4861 gagcttctcg atgaggtcga tgtggcggcg gaacacgtgg acacgctctt cgagcgtcgg
     4921 tgctcccgga tcggggcgcc ccgcacacac ctcgcgcagc cgcgaaaaca gccatggatt
     4981 acccagcgca ccgcggccga tcatcacggc gtcgcagccg gtctcctcgc gcatgcgcaa
     5041 gaagcctgca acgtcgacga cgtcaccgtt gccgacgagc ggaatgtgcg ccgggagcgc
     5101 gtcgcggacg cggcgaatga tttcgttgtc gctcttgccc gagaagccct gcgtgcgcgt
     5161 gcggccgtgc accgtgatca tctgcgcgcc ggcttcgacc atcgcgcacg cgaactccgg
     5221 agcgttgcga ttgtcctgat tccagcctgc gcgatgcttc acggtcaccg gcacgtgcgc
     5281 gggcacggcg gcaaccacgg tgcgcaccag ctcctgcgcg atcgcaggct cgcgcatcag
     5341 cgccgagccg cactcgccgg cgaccacgcg cttggccgga cagcccatgt tgatgtccac
     5401 gagcgatgcg ccgatcgcga ccgccatctt tgcggccgca tccatcttgt cgggctcgcg
     5461 gccgaagatc tgaacgccga actgacggcc atcgagcgag gccgtcagct tcgcgctggt
     5521 cttcttgtcg ccgaccgcaa gcgcatgcgc gctcaagaac tcggtgatcg tgaagccgac
     5581 cccgagctct tccgccaccg tacgaaacgg tagatcggtg acggccgcca tcggtgcgag
     5641 cagcaccggc gtgtcgatat gaatcggacc gatgcgcacg actg
//
LOCUS       DASUFX010000506         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1886184, whole genome shotgun sequence.
ACCESSION   DASUFX010000506 DASUFX010000000
VERSION     DASUFX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886184"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFV99_20285"
     CDS             complement(<1..641)
                     /locus_tag="VFV99_20285"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5061722.1"
                     /translation="MTGSQVLLELVVVLGVAAVITLAFEALHMPAVLGYIAAGLVIGP
                     HVPVPLVANPTLVHTLSELGVILLMFALGLELRLSTLARVGIGAALTALFEVALVLAA
                     GALVAGLLGFSSGEAMFAGACLAISSTMLVAKAFEHLRWKGGFTDVVFAILVFEDLIA
                     ILLLAVLTAFASGVGLEAEELALTIAKLAGFLTALLVGGLLVVPRTIRRIKKL"
     gene            complement(1161..>2893)
                     /locus_tag="VFV99_20290"
     CDS             complement(1161..>2893)
                     /locus_tag="VFV99_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061723.1"
                     /translation="AGGLYVAKLDETGRQLSHTSLPAEPPITGMAMTSLGLLAWRADQ
                     TFALIDVDGATKERLGTEPQQRIIAVATNAKRAVVLLEREGSKRQARWLTLEPKLAWG
                     GWITLDGDLTNAQQIALSPSGKRLAMLAGVSSVGQQKQTPPVFISTMGTVFDIAKAGK
                     VLVTGLVNNAQADVGFADDDHVAFGGFQGLSWVDLKDTTPKAQPITTPASAGSRTEAM
                     LATGGGRAVSPMNGDLVISTPEKTQYLGYDTISPRIAEVGPDGQLLVSVGNELLLLDQ
                     SLKMSSLLTPFKAGTTGTIGQVAELHWLGDNDWLFESTTPSDPTMQISLVDAKGFSTQ
                     VRKGIKEVQILGYEPSSQLVTLSFGAESEVARYDRKGKKLDRIASVKKASPYEQVMFV
                     PVSAAASHGTQLVQITMREKSTVKWFRDAGALDKPSATVTVEGPFAGADAAGHVFMWR
                     NTPTGQLELVLYADGKPLRTLPNNGAVALWPEPSGKRYVEVAQSSVALYDLDGKQLWY
                     QQIATSQEALWLTDGAIAITSAGGVARLDPATGAVTAARCGWRFSLADKPHPAPPRVE
                     PLCSQLRRSL"
BASE COUNT          512 a          977 c          933 g          471 t
ORIGIN      
        1 tcgagcttct tgatccggcg gatcgtgcgc ggcacgacga gcaggccacc gacgagcagt
       61 gcggtgagga agccggcgag cttcgcgatc gtcaacgcga gctcttccgc ttcgagcccg
      121 acacccgacg cgaacgcggt cagcacggcg agcagcagga tcgcgatcag atcctcgaac
      181 acgagaatcg cgaacacgac atcggtgaag ccgcccttcc agcgcaggtg ctcgaacgct
      241 ttcgcgacca gcatcgtgct cgagattgcg agacacgcgc cggcgaacat cgcttcgccg
      301 ctcgaaaacc cgagcaagcc ggcaaccaac gcaccggctg cgagcacgag cgcgacctcg
      361 aacagcgcgg tgagcgcggc accgataccg acgcgagcga gcgtcgagag tcgcagctcg
      421 aggccaagtg caaacatcag caggatcacc ccgagctcgg acagcgtgtg cacgagcgtg
      481 gggttcgcga cgagcggcac cggcacgtgc ggtccgatca cgagacccgc cgcgatgtag
      541 ccgagcaccg ccggcatgtg cagcgcctcg aacgcgagcg tgatcaccgc ggcgacgccg
      601 aggacgacga ccagctcgag gagaacctgc gaaccggtca tttcttcttc ttcgccaccg
      661 gatcgggaaa gtggcgctta cagcgcgggc atacccggtg cgcgaccgga gtacgcgtta
      721 ccgctttctt gtagccacac catggacact tgatcgtcat cagtcgcgcg agcgctgagg
      781 cgagggcaat tggaccaccc ataaagactc ctctgttgca tgtcgatgcc tgatcgtcgc
      841 ttgctggaga tcaagcaagc ggtggtccac gcgcacaggc gatgtcagca gcgcacgccg
      901 ctcggggagt catggagcac cacgacgtgt cccctgcaac tgtgacgggc acagcgacga
      961 tcggcgtggc cgcgagaacg aacgccgaca gccgcttttc gggcaattgc ggagcatgct
     1021 cgcgggcgac gagagccacc gccgcggggg cggcagcgat cttcgcctcc tgccgatcac
     1081 gtagctcgac gtgcagcggg cgcacggcgg tcgccaccac acaggcgacg accaggaccc
     1141 aagtgcaccg catgctcaca ctatagcgaa cgtcgcagct gagagcacaa gggttcgaca
     1201 cgcggaggcg ccggatgagg cttgtcggcg agcgagaacc gccagccgca gcgggccgcg
     1261 gtgaccgcgc cggtcgccgg atcgagccgc gccacaccac ccgcgctcgt gatcgcgatc
     1321 gctccgtcgg taagccacag cgcttcctgc gacgtcgcga tctgctggta ccagagctgc
     1381 ttgccgtcga ggtcgtagag cgcgacggag ctctgcgcga cctcgacgta acgcttgccg
     1441 ctgggctccg gccacagtgc gaccgcgccg ttgttcggca gcgtgcgcag cggcttgcca
     1501 tcggcgtaga gaacgagctc gagctgaccg gtcggcgtgt ttcgccacat gaacacgtgg
     1561 ccggccgcat cggcgccggc gaacggccct tcgaccgtga ccgtcgcact cggcttgtcc
     1621 agcgcaccgg cgtcgcggaa ccacttcacc gtcgatttct cgcgcattgt gatctgcacg
     1681 agctgggtgc catgcgacgc tgcggccgag accgggacga acatcacctg ctcgtacggg
     1741 ctcgccttct tcacgctcgc gatacgatcg agcttcttgc ctttgcgatc gtagcgcgcg
     1801 acctccgact cggcgccgaa cgacagcgtc acgagctggc tgctcggctc gtagccgagg
     1861 atctggactt ccttgatgcc ctttcgcacc tgcgtactga agcccttcgc gtcgacgagc
     1921 gagatctgca tcgtcgggtc gctcggcgtc gtgctttcga acaaccagtc gttgtcaccg
     1981 agccagtgca gctcggcgac ctgaccgatg gtcccggtgg tgcccgcctt gaacggggtg
     2041 agcagcgagc tcatcttcag actctggtcg agcagcaaca gctcgttgcc gacgctgacc
     2101 agcagctgac catcgggacc gacctcggcg atccgcggcg agatggtgtc gtagccgagg
     2161 tactgcgtct tctccggcgt cgagatcacg aggtcgccat tcatcggcga caccgcgcgg
     2221 ccaccgccag tcgcgagcat cgcctcggtg cgcgagcccg ctgatgccgg cgtcgtgatc
     2281 ggctgcgcct tcggcgtggt gtccttgaga tcgacccacg acaagccctg aaagccgccg
     2341 aacgcgacat ggtcgtcgtc ggcaaagccg acatcggcct gcgcgttgtt gacgaggccg
     2401 gtcaccagca ccttgcccgc cttcgcgatg tcgaacaccg tccccatcgt cgagatgaag
     2461 accgggggcg tctgcttttg ttgtccaacc gagctaacgc cagcgagcat cgcgagccgc
     2521 ttgccactcg gcgatagcgc gatctgctgc gcgttggtca gatcaccatc gagcgtgatc
     2581 cagccacccc acgcgagctt tggttcgagc gtcagccagc gcgcttggcg cttgctgccc
     2641 tctcgctcga gcaacacgac cgcgcgtttc gcgttcgtgg caaccgcgat gatgcgttgc
     2701 tgcggttcgg tgccgagccg ttctttggtc gcgccgtcga catcgatcaa cgcgaacgtc
     2761 tgatcggcgc gccacgcaag tagtccgagt gacgtcatcg ccatgccggt gatcggtggc
     2821 tcggccggca gcgatgtgtg cgagagctgg cgcccggtct cgtcgagctt ggcgacgtac
     2881 agaccaccag cct
//
LOCUS       DASUFX010000507         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_635907,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000507 DASUFX010000000
VERSION     DASUFX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635907"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFV99_20295"
     CDS             <1..981
                     /locus_tag="VFV99_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061724.1"
                     /translation="GAGATSDRELAFWAAELDYRSGDYRAANDEYKALLAEAAPQFRG
                     RIYDHYSSVLLYLDEPDEALRVGTLYRDAFPGEADAVGVYATTLAAAGRIDDAVTVAE
                     DALRLNEGEDTLAGLAKVLALKGDHARAKELYQQSIERAGTTRRPVRRAALALLQWMD
                     GEVDAAKQTVAPCLRTRGGPVTSDAGARERGACLFVAGVIDPAQAEAIAQELDALAAE
                     AMPTKPAYGAPSSLATLVRARAQFFGGGCVIAPTGGTAPGDVDEKAYDVPLDFYAAYH
                     VPFFATWAVCERAALRATRGDKAGARALLEPIAKRAPNRTWLTATAQRYQ"
     gene            complement(953..>2287)
                     /locus_tag="VFV99_20300"
     CDS             complement(953..>2287)
                     /locus_tag="VFV99_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061725.1"
                     /translation="RGAFESTFTRVHPASGVATYSHLENEYLQAVVDWGALGGLALAF
                     AAIWLAVVAVKRWRDGPLAAGALGALTVVAIQSNVDFGIEFLGLAAPITAVAATLVYV
                     PLREITHVGRVRTLRGAHIFALALMAVLLFSAKTTLLDEDRQALTDRPTFAEVRASVQ
                     RHPLDYYTYAVAAELLERGHDPRAIRLLNHAMLLHPTHPGLHRMAARMLYHDGFVGQS
                     TIEYAAALRFTPQPEKLIGEIVNTFPREQAATALPLDYSELELLTRTLTDVGHTDVAT
                     LWLARVLDQRPNYSRACEQLFVIAQRGDLPAAQVAGKRCGEMLPDYQTRLAIAKLLAD
                     KHGYVEVIELLNDVEGWESRVDDKINGWLALCDAHAGLNHVDDAKRCLRRLDSSPDMR
                     AERRNEILKRLDTLQTPSELPGAGSATPPATPATGSAAQPTTPATGSAAPSR"
BASE COUNT          391 a          726 c          800 g          370 t
ORIGIN      
        1 ggggcagggg ccacgagcga ccgcgagctc gcgttctggg cggccgagct cgactaccga
       61 tcgggcgact accgcgccgc gaacgacgaa tacaaggcgc tactagcgga ggcggcgccg
      121 cagtttcgcg gccggatcta cgatcactac tcgtcggtgt tgctctacct cgacgagccc
      181 gacgaagcgc tgcgcgtcgg cacgctgtat cgcgacgcgt ttccgggcga agccgatgcc
      241 gtcggcgtgt atgccacgac actcgccgcc gccggtcgta ttgatgacgc ggtgacggtc
      301 gccgaggacg cgctgcgcct caacgagggc gaggacacgc tcgctggtct cgcgaaggtg
      361 ctagcgctca aaggtgatca cgcgcgcgca aaggagctgt accagcagtc gatcgagcgc
      421 gcgggcacga cacgacggcc agttcggcgt gcggcgctag cgttgttgca atggatggac
      481 ggcgaggtcg acgcggcgaa gcagaccgtt gcaccctgtt tgcgtacgcg cggaggtccg
      541 gtaaccagtg atgccggtgc acgcgaacgc ggtgcctgtt tgttcgtggc gggcgtgatc
      601 gacccggcgc aggccgaggc catcgcgcag gagctcgacg cgctcgccgc cgaagcgatg
      661 ccgaccaagc ccgcttacgg tgcgccgtcg tcgctcgcga cgttggtgcg tgcgcgcgcg
      721 cagttcttcg gcggcggctg cgtgatcgcg ccaaccggtg gcacggctcc aggtgatgtc
      781 gacgagaaag cgtacgacgt gccgctcgac ttctacgcgg cgtatcacgt gccgttcttt
      841 gcgacgtggg ctgtgtgcga gcgcgcggcg ctgcgagcga cgcgtggcga caaggctggc
      901 gcgcgcgcgt tactcgagcc gatcgcgaag cgcgcgccga accggacgtg gcttaccgcg
      961 acggcgcagc gctaccagta gccggcgtcg tcggttgcgc agcgctacca gtagctggcg
     1021 tcgctggcgg cgtagcgcta cccgcgccgg gaagctcgct gggcgtttgc agcgtgtcga
     1081 gacgcttgag gatctcgttg cggcgttcgg cgcgcatgtc gggggacgag tcaagtcggc
     1141 gcaggcaacg cttggcatcg tcgacatgat tcagcccagc gtgggcgtca cacagcgcca
     1201 gccagccatt gatcttgtcg tcgacgcgac tttcccaacc ttcgacatcg ttcaataact
     1261 cgatcacctc gacgtagccg tgcttgtcgg cgagtagctt tgcgatcgcg agtcgcgtct
     1321 ggtaatccgg cagcatctct ccgcagcgct tgccggcaac ctgggccgcc ggcagatcac
     1381 cgcgctgcgc gatgacgaac aactgctcac acgcgcgtga gtagttcggg cgctgatcga
     1441 ggacgcgcgc gagccatagc gtcgcgacat cggtgtggcc gacatcggta agtgtgcggg
     1501 tgaggagctc gagctcggaa tagtcgagtg gcagcgcagt agctgcttgc tcgcgcggga
     1561 acgtgttgac gatctcgccg atcagcttct cgggctgcgg tgtaaagcgc agcgcagcgg
     1621 cgtattcaat cgtcgactgg ccgacgaagc catcatgata gagcatgcgc gcggccatgc
     1681 gatggaggcc gggatgcgtc gggtgcagca gcatcgcgtg gttcagcaga cgaatcgcgc
     1741 gcggatcgtg cccgcgttcg aggagctcgg ccgcaaccgc gtaggtgtag tagtcgagtg
     1801 gatgacgctg cacggaggca cgtacctccg caaacgtcgg ccgatcggtc aacgcctgcc
     1861 gatcttcgtc gagcagcgtc gtcttcgccg agaacaacaa cacggccatc agcgcaagcg
     1921 cgaaaatgtg tgcaccgcgc agcgtgcgca cgcggccaac atgggtaatt tcgcgcagcg
     1981 gcacgtagac gagcgttgcc gcgacagcag tgatcggcgc cgccaggccg aggaattcga
     2041 taccgaagtc cacgttgctc tggatcgcga cgacggtcag cgcaccgagt gcgccggcgg
     2101 ccagcggtcc gtcacgccac cgtttcaccg cgacgaccgc aagccagatc gccgcgaacg
     2161 cgagtgcgag cccaccgagc gcgccccaat cgacgaccgc ctgcagatac tcgttctcga
     2221 ggtgcgagta tgtggcgaca ccactcgctg ggtgcacgcg cgtgaacgtc gactcgaacg
     2281 cgccgcg
//
LOCUS       DASUFX010000508         5795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1610426, whole genome shotgun sequence.
ACCESSION   DASUFX010000508 DASUFX010000000
VERSION     DASUFX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610426"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3106
                     /gene="carB"
                     /locus_tag="VFV99_20305"
     CDS             <1..3106
                     /gene="carB"
                     /locus_tag="VFV99_20305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5061726.1"
                     /translation="INSNPATIMTDPELADATYVEPLTVEVLDKVLAREKPSAILPTL
                     GGQTGLNLAMAASKAGLLEKHGVELLGATVKTIEMAEDRELFKQAMARIGLQCPRSRL
                     VTSVEEARAVMDEIGFPAILRPSFTLGGSGGGIAYNKPDFDRMVQFGLDQSPVHSILV
                     EESVLGWKEYELEVVRDRKDNAVIVCSIENVDPMGVHTGDSITVAPAMTLTDKEYQRM
                     RDASIAIMREIGVTTGGSNVQYAVDPKTGRMLVIEMNPRVSRSSALASKATGFPIAKI
                     AAKLAIGYTLDELKNDITRVTPASFEPTIDYVVVKWPRFAFEKFPSAKPVLGPQMKSV
                     GEAMSIGRTFREALGKAIRSLETGRAGFDLPMTSLGDDLQAIERAIAVASPERLFQVG
                     RAFQLGLTIERAHELTRIDPWFLHHIREIAVAEMSLAGKSIAALGAELRRYKRLGLSD
                     RRIAALTGATEAQVREARHAAGIRPVYKRVDTCGGEFESHTPYMYSTYEDECESRPST
                     NRKVIILGGGPNRIGQGIEFDYCCCHAAMALKEEGIESIMVNCNPETVSTDYDTSDRL
                     YFEPLTLEDVLEICDKEKPWGVIVQFGGQTPLKLAVPLAAAGVPIIGTSADAIDRAED
                     RERFGDVMKKLDLHAPSWGIARSLDEARRVASQIGFPVMVRPSYVLGGRAMERVYDAR
                     HLEDYFSRVLPAAHGGRGSDEGASVGFPLLIDQFLADAVELDVDVVADKTGAVVVGGV
                     MEHIEEAGIHSGDSACALPPYSLPADIIDEVKRQARMLAIELGVIGLMNVQFAIHDGE
                     VFVLEVNPRASRTIPFVSKAIGRPLAKIAAKIMTGKTLAELGLTEIEPRHVSVKEVVF
                     PFVKFEGVDTILGPEMRSTGEVMGIDSDFARAFAKSQLAAGMKLPTRGMAFLSVREAD
                     KPALPAIARRLVTLGFKLCATHGTAAYLKAHGLADVAGINKVLEGRPHCVDAMDNHEI
                     DFVINTTEGAQAIHDSQSLRRAALMNGIAYYTTLRAAKAALEAIAVEKRGDMRVQSLQ
                     IYAR"
     gene            3107..4057
                     /locus_tag="VFV99_20310"
     CDS             3107..4057
                     /locus_tag="VFV99_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061727.1"
                     /translation="MAARSLEERLAALRAVDATTPQGKKALREAIKSTTGILIATAAK
                     HVAEHNLADLVPDLVDAFERLCGDQAVKRDPGCRGKTAIARALHALDHWDDRIFVAGL
                     RVVQREGFDGEDMAAELRGICGLAHAHFARNDALDVLAELLVDRTRTARVAAAQAIGD
                     AGRADASALLRLKLRIGDDDPEVLAACIESLLSLSREAAHDFVIGLLAKHDDRAEAAA
                     LALGGARIASALEPLRAWCLGCSAEQRRRVGYLAIALLRSDDGNAYLLDLVSTAARGD
                     AVAAAQALATFKETIADKLRSAARAHKDVAVRRDVDALLT"
     gene            4189..4725
                     /locus_tag="VFV99_20315"
     CDS             4189..4725
                     /locus_tag="VFV99_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061728.1"
                     /translation="MIRGLRAARVMLISLVLACGHGAAPKTAGDRTTPDRPNCVELVV
                     GTAREEAVLCEETDYNYWTVTHQVVRVVRAGKTVPVLDLLTKIEAFDSGAIWLEQRLR
                     IGADGMSAILDGVPITDVSARHTGQPGPFEDCNHASDWKGSPIEGEPKYRDLRRQSCN
                     ALGAYQWNGGRFVHQRGR"
     gene            4909..5766
                     /locus_tag="VFV99_20320"
     CDS             4909..5766
                     /locus_tag="VFV99_20320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5061729.1"
                     /translation="MSPTKPAPEKKSGHLAVNGVNYYYEVHGSGEPLLLLHGGLMSID
                     SFGPVLTKLAETRQVIAVDLQGHGRTDLGTRKMSVPDMGDDMSAILYKLGYKQVDAMG
                     YSLGAATAFRLAIQHPNQVRRLVLLSAAFSRDGFYPEMLPLQAQVSSAALPMMKNTPM
                     YQSYVKIAPHPEDFGTLLDRIGELMRTNYDWRDDLEKLSQPTLLVWGDSDMMRPEHMV
                     ESYKAIGGGQKDAGWQRENMSKNRLAVLPNATHYDILESPSLVPTVLPFVDGYPAGPT
                     SEAQVATKQ"
BASE COUNT         1056 a         2025 c         1797 g          917 t
ORIGIN      
        1 gatcaactcg aatccggcga ccatcatgac ggacccggag ctcgccgacg cgacgtacgt
       61 cgagccgctc accgtcgagg ttttggacaa agtgctcgcg cgcgagaaac cgagcgcgat
      121 cttgccgacg ctcggtggcc agaccggcct caatctcgcg atggccgcga gcaaagctgg
      181 actcctagaa aagcacggtg tcgagcttct cggtgcaaca gtgaaaacca tcgagatggc
      241 cgaggatcgc gagctgttca agcaagcgat ggcgcggatc ggcctccagt gcccccgctc
      301 ccggctcgtc acgtcggtcg aagaagcgcg cgcggtgatg gacgagatcg ggttccccgc
      361 gatcctgcgg ccgtcgttca cgctcggcgg ctcgggcggc ggcatcgcgt acaacaagcc
      421 cgacttcgat cgcatggtgc agttcggtct cgatcagagt ccggtgcact cgatcctcgt
      481 cgaagagagc gtgctcggct ggaaagagta cgagctcgaa gtcgttcgcg accgcaagga
      541 caacgcggtc atcgtctgct cgatcgagaa cgtcgatccg atgggcgtgc acaccggcga
      601 ctcgatcacg gtcgcgcccg cgatgacgct gaccgacaag gagtaccagc gcatgcgcga
      661 cgcctcgatc gcgatcatgc gcgagatcgg cgtcacgacc ggtggcagca acgttcagta
      721 cgccgtcgat ccgaagacgg gccgcatgct cgtcatcgag atgaacccgc gcgtgtcgcg
      781 cagctcggca ctcgcgtcga aggcgactgg cttcccgatc gcgaagatcg ccgcgaagct
      841 cgccatcggc tacacgctcg acgagctcaa gaacgacatc acgcgcgtca cgcccgcatc
      901 gttcgagccg acgatcgatt acgtggtcgt caaatggccg cgcttcgcgt tcgagaagtt
      961 cccgtcggcc aagccggtgc tcggcccaca gatgaaatcg gtcggcgaag cgatgtcgat
     1021 cggccgcacg ttccgcgaag cactcggcaa agcgattcgt tcgctcgaga ccggccgcgc
     1081 gggcttcgac ctaccgatga cgagcctcgg cgatgaccta caagcgatcg aacgcgcgat
     1141 cgcggtcgca tcgcccgagc gcctgttcca ggtcggtcgc gcgttccagc tcggcctcac
     1201 gatcgagcgt gcgcacgagc tcacgcgcat cgacccgtgg ttcctccatc acattcgcga
     1261 gatcgcggtc gccgagatgt cgctcgccgg caaatcgatc gccgcgctcg gtgccgagct
     1321 ccgccgctac aagcgcctgg gcctgagcga tcgccgcatc gccgcgctga ccggcgccac
     1381 cgaagcgcag gtgcgcgagg cacggcacgc cgccggcatt cgcccggtct acaagcgcgt
     1441 cgacacctgc ggcggcgagt tcgaatcgca cacgccgtac atgtattcga cgtacgaaga
     1501 cgagtgcgag tcgcgcccgt cgacgaaccg caaggtgatc atcctcggcg gcggtccgaa
     1561 ccgcatcggc caaggcatcg agttcgacta ctgctgctgc catgccgcga tggctctcaa
     1621 ggaagagggc atcgagtcga tcatggtcaa ctgcaacccc gagacggtct cgaccgacta
     1681 cgacacctcc gatcgcctct acttcgagcc gctcacgctc gaggacgtgc tcgagatctg
     1741 cgacaaggaa aagccgtggg gcgtgatcgt gcagttcggc ggtcagacgc cgctcaagct
     1801 cgccgtccca ctcgccgcag cgggcgtccc gatcatcggc acgagcgccg acgcgatcga
     1861 tcgcgctgaa gatcgcgagc gcttcggcga cgtgatgaag aagctcgatc tccacgcacc
     1921 gtcgtggggc atcgcgcgaa gcctcgacga ggcccgccgc gtcgcgagcc agatcggctt
     1981 cccggtcatg gtgcgcccga gctacgtgct cggcggtcgc gcgatggaac gcgtctacga
     2041 cgcgcgccac ctcgaggact acttctcgcg cgtgctccct gcagcacacg gcggccgcgg
     2101 cagcgacgaa ggcgcgagcg tcggcttccc gctactcatc gatcagttcc tcgccgatgc
     2161 ggtcgagctc gacgtcgatg tcgtcgccga caagactggc gcagtcgtcg tcggcggtgt
     2221 gatggagcac atcgaggaag ccggcattca ctccggcgac tccgcctgcg ccctgccgcc
     2281 ctactcgctc cccgccgaca tcatcgacga ggtcaaacgc caagcacgca tgctcgccat
     2341 cgagctcggt gtcatcggcc tcatgaacgt gcagttcgcg attcacgacg gcgaagtgtt
     2401 cgtgctcgag gtcaacccgc gcgcgtcgcg cacgattccg ttcgtcagca aggcgatcgg
     2461 tcgcccgctc gccaagatcg ccgcgaagat catgaccggc aagacgctcg ccgagctcgg
     2521 gctcaccgag atcgagccgc gccacgtctc ggtcaaagag gtcgtgttcc cgttcgtgaa
     2581 gttcgaaggc gtcgacacca tcctcggtcc cgaaatgcgc tccaccggcg aggtcatggg
     2641 catcgactcc gacttcgcgc gcgcattcgc caaatcccag ctcgccgccg gcatgaagct
     2701 gccgacgcgc ggcatggcat tcctgtccgt ccgcgaagcc gacaagcccg cgctccccgc
     2761 catcgcgcgg cgcctcgtca cgctcggctt caagctgtgc gcgacgcatg gcaccgcggc
     2821 ctacctcaag gcgcacggcc tcgccgacgt cgcgggcatc aacaaggtgc tcgaaggtcg
     2881 cccgcactgc gtcgacgcga tggacaacca cgagatcgac ttcgtcatca acacgaccga
     2941 aggcgcccaa gccatccacg actcgcagtc gctgcgccgc gccgcgctga tgaacggcat
     3001 cgcgtactac acgacgctac gcgccgcgaa agccgcgctc gaagcaatcg ccgtcgagaa
     3061 gcgcggtgac atgcgcgtcc agtcgctgca aatctacgcg cgctgaatgg ctgcgcgcag
     3121 cctcgaagag cggctcgccg ccctacgcgc cgtcgacgcg acgacaccgc aaggcaagaa
     3181 agcgctgcgc gaggcgatca aatccaccac gggcattctg atcgcgaccg ccgccaagca
     3241 cgtcgccgag cacaacctcg ccgacctcgt gccggacctc gtcgacgcgt tcgagcgcct
     3301 gtgcggcgat caagccgtca aacgcgaccc tggctgccgc ggcaagaccg cgatcgcccg
     3361 cgcactacac gctctcgatc actgggatga ccgcatcttc gtcgccggcc tccgcgtcgt
     3421 gcagcgcgaa ggtttcgacg gtgaagacat ggccgccgag ctgcgcggca tctgcggcct
     3481 cgcgcacgcg cacttcgcgc gcaacgacgc tctcgatgtc ctcgccgagc tgctcgtcga
     3541 ccgcacgcgc accgcgcgcg tcgcggccgc ccaagcaatc ggcgatgcgg gccgcgcgga
     3601 cgcgagcgcg ctgctgcgcc tcaagctccg catcggcgac gacgatccgg aggtgctcgc
     3661 cgcgtgcatc gagtcgctgt tgtcgctctc ccgcgaagcc gcgcacgact tcgtgatcgg
     3721 tctactcgcc aagcatgacg acagggcaga agccgcggca cttgcgctcg gtggtgcacg
     3781 gatcgcgagc gcgctcgagc cgcttcgcgc gtggtgcctc ggttgctcgg ccgaacagcg
     3841 tcgccgcgtt ggctatctcg cgatcgccct actgcgctct gacgacggca acgcctacct
     3901 gctcgacctc gtgagcaccg ccgcgcgtgg tgacgcggtc gcggcggcgc aagcgctcgc
     3961 gacgttcaag gagaccatcg cagacaagct gcgcagcgca gctcgcgcgc acaaggacgt
     4021 agcggtgcgc cgcgacgtcg acgcacttct cacctgactc gcgttccccc gccccgtcgg
     4081 gtatgtgaga cgcgccaaaa gaaaacgtcc ttccacgcga ccacccgcgt taaatgagtg
     4141 accggcgtga tcaccattcc tacaatcagt ggcgcctgcg gattaccagt gattcgcggc
     4201 ctacgcgctg cgcgggtgat gctgatctcg ctcgtgctcg cgtgcggtca tggagctgct
     4261 ccgaagaccg caggcgatcg aacgacgccc gaccggccga actgcgtcga gctcgttgtc
     4321 ggtacggctc gagaagaagc cgtgctgtgc gaagagaccg actacaatta ttggacagtc
     4381 acgcaccaag tcgtccgcgt cgtgcgcgcg ggcaagaccg tgcccgtgct cgacctgctg
     4441 acgaagatcg aggcattcga cagtggcgcg atctggctcg agcagcggct aagaattggc
     4501 gccgacggca tgtcagcgat cctcgatggt gtgccgatta ccgatgtctc ggcacgacac
     4561 actggccaac cagggccatt cgaagactgc aaccacgcct ccgattggaa gggctctccc
     4621 atcgagggcg agccgaagta ccgcgacttg cgtcgtcaat catgcaacgc gctcggtgct
     4681 taccaatgga acggcggccg ctttgtccat cagcgaggac ggtagacgcc gccggcctaa
     4741 cagccgggat cacacgggtt taatgatttc ttcgatcagc tgtcgaatcg gcgatcgctc
     4801 gttcgtcgtc tggtcatgaa ggcgacaacg accctcctga tttccctcct cctcgcagcg
     4861 gccgcgatcg gctgccactc ctccgaagcc cagaaaggac ctgcccccgt gtctccgacc
     4921 aagcccgccc ccgagaagaa gtccggtcac ctcgccgtca acggcgtgaa ctactactac
     4981 gaagttcacg gcagcggcga accgctgctc ctcctgcacg gcggcctcat gtcgatcgac
     5041 tcgttcggcc ctgtgctgac gaagctcgcc gagacgcgcc aggtgatcgc cgtcgacctc
     5101 cagggtcacg gccgcaccga cctcggcact cgcaagatga gcgtccctga catgggcgac
     5161 gacatgtccg cgatcctcta caagcttggc tacaagcaag tcgacgcgat gggctactcg
     5221 ctcggcgccg ccaccgcgtt ccggctcgcg atccagcatc ccaaccaggt gcgccggctc
     5281 gtgctcctct cggccgcgtt ctcgcgcgac ggtttctatc ccgagatgct gccgctccag
     5341 gcgcaggtct cgtcggccgc gctgccgatg atgaagaaca cgccgatgta tcagtcgtac
     5401 gtgaagatcg cgccgcaccc ggaagacttc ggcaccctgc tcgatcggat cggcgagctc
     5461 atgcgcacga actacgactg gcgcgacgat ctcgagaagc tctcgcagcc gacgctgctc
     5521 gtgtggggcg atagcgacat gatgcgcccc gagcacatgg tcgagtcgta caaggcgatc
     5581 ggcggcggcc agaaggatgc cggctggcag cgcgagaaca tgagcaagaa ccgactcgcg
     5641 gttctgccga acgccacgca ctacgacatt ctggaatcgc cctcgctcgt gccgaccgtg
     5701 ctgccgttcg tcgacggcta cccggccgga ccgacgtcag aggcgcaggt tgctacgaag
     5761 cagtgaacgt cacgtcttcg agcgacgcac ccgga
//
LOCUS       DASUFX010000509        12799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1886553, whole genome shotgun sequence.
ACCESSION   DASUFX010000509 DASUFX010000000
VERSION     DASUFX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12799
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886553"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFV99_20325"
     CDS             <1..183
                     /locus_tag="VFV99_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061730.1"
                     /translation="ASFGHHTAAAKNGVRAPDTNFELEIGEMDYGFSLGGILGMDFLR
                     AAGARIDLDHLTIDFA"
     gene            complement(164..961)
                     /locus_tag="VFV99_20330"
     CDS             complement(164..961)
                     /locus_tag="VFV99_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061731.1"
                     /translation="MKVWRTRDGYLAEHGGDFVVVSTDGLVVRWFGSRAYSDEDDLWH
                     WQHLVIGTALYERTTVERGPLVTKTEDQLVVDGLEAIEERHVIELVERALATDRDARQ
                     QEMLRAQRAGDARVATIVGGLRDGELGPELVRARDALAARVRAYDDALAARLLGTLVL
                     VARVRPGSTVEATYAQGCLAACFQPAADERDGEPAGTPLREPQAALAAELALRAAELE
                     QQAEHNEYIDASRTATAYFRAAAAIREAARVAAGTSDDVSRTTRSRS"
     gene            complement(1559..2680)
                     /locus_tag="VFV99_20335"
     CDS             complement(1559..2680)
                     /locus_tag="VFV99_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061732.1"
                     /translation="MRRFSGDGGRFDGQSPTIRISDAIEGNRLVQLVAHESSHLSMYQ
                     LTRGASATEPFRFFDEGYSDLVGKMIAGENMANHKRSSLAIANREVKTKGVGFETMQH
                     WSDYAGNVPSPKQAYAYPVGSSFVYFVIDSFGEKKLFDFFRDIGETRDLDTSLRNIFG
                     LTAVEVEQRWKDYVASSALEPPAITEMSPSNESSNVPVDIAELLVKFSVPMMMHNVCV
                     DAVRRHRRLLHERLVERRQDSCRQDGRQAAAGDHLQARAEPWQARLSANERGWDSLAH
                     DTMGFHHEQADVKRADRSIRERIGGGGGSRTTWDNLSRRVLARFFETNDPSGSRRFLP
                     VSRSLAARVAACWRLAPRLVRTPDVAFMCLHHVRVRALG"
     gene            complement(2836..3474)
                     /locus_tag="VFV99_20340"
     CDS             complement(2836..3474)
                     /locus_tag="VFV99_20340"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEX5061733.1"
                     /translation="MIGRIWYVLTALTVVTALAFHVAIAAEGDPQFASAAASVANMFT
                     YFTITSNVIVGVVSALLASGRARGESWLSALCVAGLTAITITAAVFHVVLASAIPALS
                     GAPAVANTLVHTVVPTVFIAGWAAFGPRGTVHVRTVLLSLIYPVTWLAFTLIRGVLSD
                     YYPYPFMDATQIGYAQMALNVTLVAVLYVVLAVAFGAIDRALAHRSSAAQVP"
     gene            complement(3475..4077)
                     /locus_tag="VFV99_20345"
     CDS             complement(3475..4077)
                     /locus_tag="VFV99_20345"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5061734.1"
                     /translation="MPGEGAIPRPGAPSATLRQLEHHASPAWIDACSPPTSEYQHVLR
                     VEPDWLRVREHARLHDRAEPSKRSSFGFAPKPRPRWLNGQRSPTTKRRRRRPGSHHRK
                     LPRIWPLFAFYGLYMALTDGVGKALLADHAPRDRRGRALGIFYLAQGITTIASSVVAG
                     LLWDHVDPSAPFWFGASGAVVALAILPLAARAARRMQKAT"
     gene            4076..4573
                     /locus_tag="VFV99_20350"
     CDS             4076..4573
                     /locus_tag="VFV99_20350"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX5061735.1"
                     /translation="MKRSSRSRALVGLGLTALCFGIAALGGAATVRGRGRPDGPWFRS
                     LRKSPLQPPARVFGPVWTVLYGLIAYSGYRIWRAPRSRQRTRALALWGTQLALNGAWT
                     PLFFGARRPVAALADLVALDAVAGAYAVVAREVDPGAALAVTPYLAWSGFATYLNGSI
                     VARNR"
     gene            complement(4825..6543)
                     /locus_tag="VFV99_20355"
     CDS             complement(4825..6543)
                     /locus_tag="VFV99_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061736.1"
                     /translation="MEANVLIVTSSGAPPSAVVPVLAAIEAAGMRVRAIDVGAIGTGG
                     TGVADRMRRALLGENAERKLRRELEISPPDAAIVFDPHSALALTVARDHSQNPAPVIA
                     VVGDLDPVPAWSQTDADRFIAIDELAAVALADAGVEGDRILVVGAIGERAFAEAGMQD
                     RTALRSRFKLQGKVALVEVAGLGAELTGQLSLQLSLLDSGSAITFLFDAAGDADAAAV
                     LRRQVPTLGLRGKLFGATADAALLWRAADVIVARPRPEVVSRVMLVGGRLVALIDDQV
                     ANGPRVAAALEARRRAISAKGLLMLSSALDSAFGGSLPGATADGADQIADIVAAVAGD
                     KRAVIDERRNAAQAATRDRVQKASAAAHAAAAQTAMPGELEDLGGGGGGFAEAVADLP
                     DKAELERLRSEVAQRMTELNKAMTAARDAGNDAAEKAKGAAARGAADEARQLERKADS
                     ERARMHALLSELATLETELKDLERTLKDLGDVPRASASASTGGGQKSSGPQAPPRPPR
                     SSIDDELEKLKRAGGGAAPKSSAPKGGSGRTQSSGGSGGGPKNVDDELAALKKKMQNP
                     PPKKKP"
     gene            6653..6844
                     /locus_tag="VFV99_20360"
     CDS             6653..6844
                     /locus_tag="VFV99_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061737.1"
                     /translation="MRHSQLGVAALAVIGAILAVLGVLAIAMAPQIMNHAEIDTLRII
                     GGVAAGLGVVLLGVGMKKK"
     gene            complement(6854..7813)
                     /locus_tag="VFV99_20365"
     CDS             complement(6854..7813)
                     /locus_tag="VFV99_20365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX5061738.1"
                     /translation="MRIVSTSSLPIDLSAQVRAQLPDAIVDVPEQWIGVARCDLSTAD
                     ALVCLLLDRIDESVLARAPKLRVIANCAVGYDNVDIAAATRAGIAVTNTPDVLTDATA
                     ELTIALMLAAARRLPEGEAFVRSGQWPGWRLDQLIGQPIKGRTLGIVGLGRIGTAVAE
                     RALGLGMRVIYCGHTDVATPFAAQRVAVDDLFAHADVVSLHCPLTPETKHLVNAQRLA
                     RMKPTAILINTARGGIVDDGALAEALGAGRIFGAALDVFNGEPALDPRLLAAPRLVVA
                     PHLGSATTDARRQMAQLCADGVISVLTGNRPANLVNRDVVLRE"
     gene            7862..8800
                     /locus_tag="VFV99_20370"
     CDS             7862..8800
                     /locus_tag="VFV99_20370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5061739.1"
                     /translation="MRWAAIAILLFGLSTTADATSRKRPAIKAEPNKFTDDPVVGRGD
                     RINGEEVQGLVAFTFDDGPNPDTTPAVLDALKQYDIPATFFVVTRRLLGKVGEKSREL
                     LARQMSEGHLVESHSVTHAHLGKADAKKLDFEMNKSFEMLSNEAKRPVGMFRAPFGAL
                     GGAGRVRLRQLGVTEVFWSIDTLDWKAKHADKLRKKVLAMILKQKGGVVLMHDVKPIT
                     AKIVAEVFDDLEAENCRRLAAKEEPIWPVSIHYFLRDGKAPRAVPDEVAKRTEAYKKG
                     LPERCAKRPAPEPATTPAAATASAPPAAAGSPAPAK"
     gene            complement(8730..11618)
                     /locus_tag="VFV99_20375"
     CDS             complement(8730..11618)
                     /locus_tag="VFV99_20375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5061740.1"
                     /translation="MATAAAHTAVLPRAPRRLQSFFHYYPRAAAVAASIFGLIVLLGG
                     LLNIVAITRLSAPSPVTVPNSAFMGVLAGVSLFLLASAQWGRGARVAGRTVAAILALM
                     LLATLVEYVTGVDLGLDHWLADVEHAPWPVGRPSPYTVAAYLLVSSALLLGERKSRRG
                     RYFADYLAVAAAAFAMAALLGFLFGVEAHVVPYVGMSPTTVLISLAVSIGILGLHVED
                     GLISIVLAPDAGGVVARRLLVGLVLFPPVAAILILGARFQLYALPLAAALTLLFALAE
                     SSVFILNTARRLSMIDAVQRELTEDLQRSEARVRLLVEEAKDGIFLADLDGKYIEVNP
                     AACELSGYTREQLLGMTIADLAAPEERSKLAEERANIIAGKTSRREWRLRRADGSMIE
                     VEVVANVLPDRRWQAFVRDITERKRHARLLADALAAERALRADLEAVDRAREVVTEAA
                     AELRGADIDPVLAQIAEQARALTNATYAAVGIGGDESRPFEHWATSGMSAETQAAIGR
                     PPRSVGMLAHASRSLEPLRVREITEHPAFIGLPPNHPELHSFLAVSIRQRGEVVGNIF
                     LANKRDSAEFTDRDERLAGLLADGVALVIETARSYAWLQSIVDQLPDGVMIVGEHRQV
                     FAINRAMRGMARDPGVTNGDAFANPALFEACTATGELIPHEDLPLPRVLRTGETVTNE
                     ELCLRQPDGTLLPVEARAVPLRGERGRPADAVAVVHNIAERKETERLREEWIALVAHD
                     LRQPVQAIMLSFDMLFEHANEQDRALLMRVRTAAYHLNRLVGDLLDAARITAGRLAVE
                     CTYGNITTIVKEAIDLCHLTNRDIDIVLDAPPEQLAWVDPERIQQVLGNLLSNAAKYR
                     TPDTPVRVHVHVPEPKTVLVCVCNEGPPLDPDDLAGLFGRFARTRTARGKHGIGLGLY
                     ISKGLVEAHGGRIWAESSGGVTKFYFTLQVPEIPPPRVVPKRSRPPAS"
     gene            11924..>12799
                     /locus_tag="VFV99_20380"
     CDS             11924..>12799
                     /locus_tag="VFV99_20380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5061741.1"
                     /translation="MFETADLGEGFGESEGERTVITAATPYDLRPKFTGRNAALAQLQ
                     TLTDKAFAEKQIAFAVVVGEPGMGKSRIVSELIARTKTKIPNIHVLQGVADENAHAYG
                     PIARAMTVRFGLQPGEDPAESRDKIQATVAEVVQASRVTEISHLLAHLLRVPFDDSPV
                     ITPLLESPQRLEARLFMALKRLLTAEAEKRPVVIVVENLELCGGDTINFLQYLAAGMT
                     DQRVAVLGTATPALWERHAAFGEGEVAPIKIELGALTPNESEQLLRELCKQLSEVPAR
                     LIGHVRNLGGSPRAIH"
BASE COUNT         2216 a         4257 c         4255 g         2071 t
ORIGIN      
        1 gcgagcttcg ggcatcacac cgccgccgcc aagaacgggg tccgtgcacc ggataccaac
       61 ttcgagctcg agatcggcga gatggactac ggattctcgc tgggcgggat cctcggcatg
      121 gatttcctgc gtgcggccgg agcgcgaatc gatctcgatc acctcacgat cgacttcgcg
      181 tagtccgcga gacgtcgtca gacgtgccag cagccacccg ggctgcttcg cggatcgcgg
      241 cagccgcacg aaagtatgca gtggccgtgc gactcgcatc gatgtactcg ttgtgctcgg
      301 cctgctgctc gagctctgcc gcgcgcaacg cgagctccgc tgcaagcgca gcctgcggtt
      361 ctcgaagcgg tgtgccggcg ggttcgccat cgcgttcgtc agcagcaggt tgaaagcatg
      421 cggcaaggca cccttgagcg tatgtggcct cgacggtcga accggggcgc acgcgcgcaa
      481 ccaagacgag cgtaccgagc aggcgagcgg ctagcgcgtc gtcgtacgcg cgcacgcgtg
      541 ctgcaagggc gtctctcgcg cgcaccaact ctggcccgag ctcgccatcg cgaagaccgc
      601 ccacgatcgt tgcaacccgt gcatcgccag cccgctgggc tcgaagcatt tcctgctggc
      661 gggcatcacg atccgtggcg agcgccctct cgaccagctc gatcacatgt cgttcctcta
      721 tcgcctcaag gccatcaaca acgagttggt cctccgtctt ggtcacaaga gggccgcgtt
      781 cgacggttgt ccgctcgtac agggcggtgc cgatgacgag atgctgccag tgccacagat
      841 catcctcgtc gctgtaggcg cgtgaaccga accaccgaac gaccaacccg tccgttgaaa
      901 caacgacgaa gtcaccgccg tgctcggcaa gatatccgtc gcgcgtccgc cagaccttca
      961 caacaccagt ctgccatgca atgaagcgcc acccgggtga tcgttctcgg ttaggcgtct
     1021 gggtagcctg accacacgta ctgcgtctcg tcgtctgcgc ccgagcaaca gaagagcgtg
     1081 cgttcgggat caggcgacag cgtcagtacg agctacacgg agacgttcgg tccgtttccg
     1141 tcgagctgag ctgcgtttcc tgtgaaaagc gcaccattgc cgccacgttc gagatcgact
     1201 acgcgccgac ggacaccttg ctgtcgcggc cgctcgagcc atgtgacgag ccgtggttga
     1261 tcccgaagcg gcaaacgtga gaaccgccgc cagcgactgc tcactcgtca ccgcacacct
     1321 ttggctccac gttcagtaac acttcgcagt cgcagtatcc gccgcggcgc ctgagccatt
     1381 gcgtgactgc gagaacgtca tatccctgat cgatcagcca cgcgatcgtt ccttgcaaag
     1441 tgttgtcgca tccgtgccga tcgatctcag cgccgagtac gtcgaacagg gagagaagcg
     1501 cttcaactgg gacgggaagg ttgttcggtg tgattagacg gctgtgcatg tcactcgact
     1561 agccgagcgc tctgacacgc acatgatgaa gacacatgaa cgcgacgtcc ggtgttcgga
     1621 caagacgagg cgccaatctc cagcacgctg ccactcgcgc tgccagactt cgcgagactg
     1681 gaaggaatcg gcgggaacca ctcgggtcgt ttgtctcgaa gaatcgggca agtactcgtc
     1741 gagaaaggtt gtcccaagtg gttcgactac cgccgcctcc accaattctt tcgcggatcg
     1801 accgatccgc ccgcttcacg tctgcttgtt cgtggtgaaa acccatcgtg tcgtgggcaa
     1861 gggagtccca cccgcgctca ttagctgaca accgtgcttg ccagggctca gctcgagctt
     1921 gtaggtggtc cccggcagca gcttgccttc cgtcctgacg gcaagagtcc tgtcgtcttt
     1981 ccaccaggcg ttcgtgtaac agacgccggt gtctccgcac ggcgtcgaca cacacgttgt
     2041 gcatcatcat gggcacgctg aacttcacga ggagctcggc gatgtcgacc gggacattgc
     2101 tcgattcgtt cgacggagac atctcggtga tcgccggtgg ttcgagcgcg gagctcgcca
     2161 cgtagtcctt ccagcgttgc tcgacctcta ccgcagtgag gccaaagatg ttccgaagcg
     2221 atgtgtcgag atctctcgtc tccccgatgt cgcggaagaa gtcgaacagc ttcttctctc
     2281 caaaggagtc gatcacgaag tacacgaagc tcgaacccac ggggtacgcg taggcttgtt
     2341 tgggtgatgg aacattcccg gcgtagtccg accagtgctg catcgtctcg aagccgacgc
     2401 ccttcgtctt cacctctcgg ttcgcgatcg cgagtgacga tcgcttgtgg ttcgccatgt
     2461 tctcgcccgc gatcatcttg ccgacgaggt cgctgtatcc ctcgtcgaag aacctgaacg
     2521 gctccgtcgc gctcgctcct cgcgtcaact gatacatgct caggtgggag gactcgtggg
     2581 cgaccagctg tacgagacgg tttccctcga tagcatcgga gatcctgatc gtgggcgact
     2641 gcccgtcgaa ccgcccgccg tcacccgaga accgtcgcac cgtgatgcgc ggtggcgtgt
     2701 tggagccgaa gacggcctcg agcctgacga ccgggcacgc gtagttttcg cgatttcggc
     2761 gtcgctctgc tcagactctg cccaaccacg accggtccct ccagatcgag gagctgcacg
     2821 gtatcgcgcg ctcgactacg ggacctgagc agccgacgag cggtgcgcga gcgcgcgatc
     2881 gatcgcgccg aacgcaaccg cgaggacgac gtagaggacg gcgacgagcg tgacgttgag
     2941 cgccatctgc gcgtagccga tctgcgtcgc gtccatgaac ggatacgggt agtagtcgga
     3001 cagcacgcca cggatcaacg tgaacgcgag ccaggtgacc ggatagatca gcgacagcag
     3061 cacggtgcgg acgtggacag tgccgcgcgg cccgaacgcg gcccagcccg cgatgaacac
     3121 ggtgggcacc accgtgtgga ccagcgtatt cgcgaccgcc ggtgcgccgg acagcgcagg
     3181 aatcgcggag gcgagcacga cgtggaatac cgcggcggtg atcgtgatcg ccgtcaggcc
     3241 cgcgacgcac agcgctgaca gccagctctc gccgcgcgcg cggcccgacg cgagcagcgc
     3301 gctgacgacg ccgacgatca cgttcgatgt gatcgtgaag tacgtgaaca tgttcgcgac
     3361 gctcgctgcc gctgacgcga actgcggatc accttcggcg gcgatggcga cgtgaaacgc
     3421 gagcgcggtg acgacggtga gcgccgtgag cacgtaccag atccgaccga tcacctacgt
     3481 tgctttttgc atgcggcgtg ccgcacgagc tgcgagcggc aagatcgcga gtgcgacgac
     3541 tgcaccggaa gcgccgaacc agaacggcgc gctggggtcg acatgatccc acaacaggcc
     3601 tgccacgacg ctgctcgcga tcgtcgtgat gccctgcgcg agatagaaga tgccgagcgc
     3661 gcgtccgcgc cggtcgcgag gcgcgtggtc ggcgagcaac gccttgccga caccgtcggt
     3721 gagtgccatg tacagcccgt agaacgcgaa cagaggccag atccgcggaa gctttcggtg
     3781 gtgcgacccg ggccggcgcc ggcggcgctt cgtcgttggc gatcgttggc cgtttagcca
     3841 tcgcggccgc ggcttcggcg cgaatccgaa cgacgacctc ttcgacggct cggcccgatc
     3901 gtgaaggcga gcgtgctcgc gaaccctgag ccagtccggc tcgacgcgaa ggacgtgctg
     3961 gtattcgctc gtaggcggtg agcaagcatc gatccaggct ggcgaggcat gatgctcaag
     4021 ttgacgcaag gtcgctgagg gtgcaccggg ccggggaatt gcaccctcgc cgggcatgaa
     4081 gaggtcatca cgctcgcgtg cgctcgtcgg gctcggcctg acagcgctgt gcttcgggat
     4141 cgctgcgctg ggcggcgccg ccacggtgcg cggaaggggc cgtccggacg gcccctggtt
     4201 ccgctcgctc cgaaaatcgc cgctccagcc gccggcgcgc gtgttcggtc cggtgtggac
     4261 cgtgctctac gggctgatcg cgtactcggg gtaccggatc tggagggcgc cgcgctcgcg
     4321 gcagcggacc cgcgcgctgg cgctgtgggg cacacagctc gcgctcaacg gcgcgtggac
     4381 gccgctgttc ttcggggcgc gccggccggt cgcggcgctc gccgatctcg tcgcgctcga
     4441 cgccgtcgcg ggcgcgtacg cggtagtggc gcgcgaggtc gatcccggcg cagcactcgc
     4501 ggtcacgccg tacctcgcgt ggagcgggtt cgcgacgtac ctgaacggca gcatcgtcgc
     4561 gcggaaccgc tgagttgccg actcgttctg acggagaccc tatccggtcg cggggcggac
     4621 gctcgaacaa gcacagcgct atgacacgca cctcgtcctt gtacgctgac gagacgtccg
     4681 gtattcggac gatgtcgagc ttgatgtcac gcatcaagcg aacacgctgc cacgcgcgct
     4741 gccagacttc gtgagactgg aaggaatcgg cggaaaccac tcgctcttgc gatagagctg
     4801 cgcgagctcg agacagagcg ccgcctatgg cttcttcttc ggcggcgggt tctgcatctt
     4861 cttcttgagc gccgcgagct cgtcgtcgac gttcttcgga ccgccgccgc tgccgccgct
     4921 cgactgggta cggccgctgc cgcccttcgg tgccgaagac ttcggcgccg cgccgccccc
     4981 tgcccgcttg agcttctcga gctcgtcatc gatcgacgag cgcggtggtc gtggcggcgc
     5041 ttgcggccca ctgctcttct gcccgccccc ggtgctcgcg ctcgccgacg ctcgcggcac
     5101 atcgccgagg tccttcagcg tgcgttcgag atccttcagc tcggtttcga gcgtcgcgag
     5161 ctccgacagc agcgcgtgca tccgcgcccg ctcggagtcc gccttgcgct cgagctggcg
     5221 cgcttcgtcg gcggcaccgc gtgcggctgc gcccttcgcc ttctcggcgg cgtcgttccc
     5281 cgcgtcgcgc gcggcggtca tcgctttgtt gagctcggtc atccgttgcg cgacttccga
     5341 gcgcaaccgc tcgagctcgg ccttgtcggg caagtcggca accgcttcgg cgaatccacc
     5401 gccaccaccg ccgagatcct cgagctcgcc gggcatcgcg gtctgcgccg ccgcggcatg
     5461 tgccgcagcc gacgccttct gcacgcgatc acgcgtcgct gcttgcgcgg cattgcggcg
     5521 ctcgtcgatc accgcgcgct tgtcgccggc gaccgccgcg acgatgtccg cgatctgatc
     5581 agcgccatca gcagtcgcgc cgggcaacga accgccgaac gccgagtcga gcgcgctcga
     5641 cagcatcagc aagcccttcg ccgagatcgc gcgccgccgt gcttcgagcg ccgccgcgac
     5701 ccgcggacca ttcgcgacct gatcgtcgat cagcgcgacg agccggccac cgacgagcat
     5761 cacgcgcgac accacttcgg gccgcggccg cgcgacgatc acgtctgcag cccgccacag
     5821 cagcgccgca tccgccgtcg cgccgaacag cttcccgcgg agtccgagcg tcggcacctg
     5881 gcgccgcaac acggcggccg catcggcatc gccggcggcg tcgaacaaga acgtgatcgc
     5941 cgagccgcta tcgagcagcg acagctgcag cgacagttgc ccggtcagct cggccccgag
     6001 cccggccacc tcgactaatg cgaccttgcc ctgcagcttg aaccgcgagc gcagcgccgt
     6061 ccgatcctgc atgccggcct cggcgaacgc ccgctcgccg atcgcaccga caacgagaat
     6121 gcgatcgcct tcgacgccgg cgtcggccag cgccaccgcc gccagctcgt cgatcgcgat
     6181 gaaccgatcg gcatcggtct gcgaccacgc cggaaccgga tcgaggtcgc cgacgaccgc
     6241 gatcacgggc gccgggttct gcgagtggtc gcgcgcgacg gtcagcgcga gcgccgagtg
     6301 cgggtcgaac acgatcgcgg catcgggcgg gctgatctcg agctcccggc gcagcttgcg
     6361 ctcggcgttc tcaccgagca gcgcgcggcg catgcggtcg gcgacgccgg tgccgccggt
     6421 gccgatagcg ccgacgtcga tcgcgcgtac gcgcatgccg gccgcttcga tcgcggcgag
     6481 caccggaacc accgcggagg gcggcgcgcc ggagctggtg acgatgagga cgtttgcctc
     6541 cacgcttgtc cctcggtggt agccgatcgg cggtgcgccg ccaagtgctg ggcgcatccg
     6601 tggaggccac gccaaagtcg cattacgggc ccagacgagc gaaactggcg gtatgcgaca
     6661 ttctcagctg ggcgtagccg ccctcgccgt catcggtgcc attctggccg ttctgggtgt
     6721 cctcgcgatc gcgatggcac cacagatcat gaaccacgcc gagatcgaca cgctgcggat
     6781 catcggtggc gtggccgccg gcctcggcgt cgtgctgctc ggcgtcggca tgaagaagaa
     6841 atagcgcgct cggtcactcg cgcagcacga cgtcgcgatt gacgagattc gcgggccggt
     6901 tgcccgtcag caccgaaatc acgccgtcgg cacagagctg cgccatctga cggcgcgcgt
     6961 cggtcgtcgc cgaaccgagg tgcggcgcaa ccaccagacg cggcgcggcg aggagccgcg
     7021 gatccagcgc tggctcgccg ttgaagacat cgagcgccgc cccgaagatg cggcccgcgc
     7081 cgagcgcctc ggcgagcgcg ccgtcatcga cgatgccgcc acgcgcggtg ttgatgagga
     7141 tggcggtcgg cttcatgcgc gcgaggcgtt gcgcgttgac gaggtgcttg gtctcgggcg
     7201 tcagtgggca atgcagactg acaacgtcgg cgtgcgcgaa caggtcatca acggcgacgc
     7261 gctgggccgc gaacggcgtc gcgacatcgg tgtgaccaca gtagatcacg cgcatgccga
     7321 gcccgagtgc gcgttcggcg acggcggtgc cgatgcggcc gaggccgacg atgccgagcg
     7381 tgcggccttt gatcggctga ccgatgagct ggtcgagacg ccagcccggc cactggcccg
     7441 agcgtacgaa tgcttcgcct tcgggaagcc ggcgagcggc ggcgagcatg agcgcgatcg
     7501 tgagctcggc tgttgcatcg gtcaggacgt cgggcgtgtt cgtgacggcg atgccggcgc
     7561 gcgtggcggc ggcgatatcg acgttgtcgt agccgaccgc gcaatttgca atgacgcgca
     7621 gcttcggcgc acgcgcgagc acggactcgt cgattcgatc gaggagcaag cagacgagcg
     7681 catcagccgt cgacagatcg caacgcgcga ctccgatcca ttgttccggc acgtcgacga
     7741 tcgcgtcagg aagttgcgcg cgcacctgcg ccgatagatc gatcggcaga ctcgatgtcg
     7801 agacaatgcg cacgtcgtga ggatactgga gatgacgaag ctgcgtgcta aggtgaatgg
     7861 aatgcgttgg gccgcgatcg cgatcttgct gttcggcctc tccacgacgg ccgatgcgac
     7921 aagtcgcaag cggcctgcaa tcaaggccga accgaacaag ttcaccgacg accccgtggt
     7981 cggtcgcggc gatcgcatca atggcgagga ggtacagggc ctcgtcgcat tcacgttcga
     8041 cgatggtccg aaccccgaca cgacgccggc cgtgctcgac gcgctcaagc aatacgacat
     8101 tcccgctacg ttcttcgtcg tgacgcggcg gctcctcggc aaggtcggcg agaagtcgcg
     8161 cgagctcctg gcgcgccaga tgtccgaggg acatctcgtc gagagccact cggtcacgca
     8221 cgcgcacctc ggcaaagccg acgcgaagaa gctcgacttc gagatgaaca agtcgttcga
     8281 gatgctgtcg aacgaggcga agcgacccgt cggcatgttt cgcgcgccat tcggtgcgct
     8341 cggcggtgcg ggccgcgtgc gcctgcgcca gctcggcgtg accgaggtgt tctggtcgat
     8401 cgatacgctc gactggaagg cgaagcacgc cgacaagctg cgcaagaaag tgctggcgat
     8461 gatcctgaag cagaaaggcg gcgtcgtctt gatgcacgac gtgaagccga tcaccgcgaa
     8521 gatcgtcgcc gaggtcttcg acgacctcga ggccgagaac tgccgccggc tcgcggcgaa
     8581 ggaagaaccg atctggccgg tctcgattca ctacttcctc cgcgacggca aggcgccgcg
     8641 cgcggtcccc gacgaagtgg cgaagcgaac ggaggcgtac aagaaggggc tgcccgagcg
     8701 gtgcgcgaag cgccctgctc cagagccggc tacgacgccg gcggccgcga ccgcttcggc
     8761 accacccgcg gcggcgggat ctccggcacc tgcaaagtga aatagaactt cgtcacgccg
     8821 cctgacgatt cggcccagat ccgaccgcca tgcgcctcga cgaggccttt cgagatgtag
     8881 aggccgagcc cgatgccgtg tttgccgcgc gccgtgcgcg tgcgcgcgaa gcggccgaac
     8941 aggcccgcaa gatcatccgg gtcgagcggc gggccctcgt tgcatacgca gacgagcacg
     9001 gtcttgggct ccggcacgtg aacatggacg cgcaccggcg tgtcgggcgt tcggtacttc
     9061 gccgcattcg agagcaagtt accgagcacc tgttggatgc gctcggggtc gacccacgcc
     9121 agctgctcgg gtggcgcgtc gagcacgatg tcgatgtccc ggttcgtcag gtgacagaga
     9181 tcgatcgctt ccttcacgat cgtcgtgatg ttgccgtacg tacactcgac ggcgagtcgg
     9241 cccgccgtga ttcgcgcggc gtcgagcagg tcgcccacca ggcggttcaa gtgataggcc
     9301 gccgtgcgca cgcgcatgag tagcgcgcgg tcttgctcgt tcgcgtgctc gaacagcatg
     9361 tcgaacgaca acatgatcgc ttgcacgggc tggcgcaggt catgcgcgac cagcgcgatc
     9421 cactcctcgc ggagacgctc ggtctctttg cgctcggcga tgttgtgcac gaccgcgaca
     9481 gcgtcggcgg gcctaccgcg ctcacctcgc aacggcaccg cgcgcgcctc gacgggaagc
     9541 aacgttccgt cgggctggcg aagacagagc tcttcgttcg tgaccgtttc acccgttcgg
     9601 aggacacggg gaagcggcag atcctcgtgc gggatgagct cgccggtcgc cgtgcaggct
     9661 tcgaacagcg ccggattggc gaaggcatca ccattggtga cacctggatc acgcgccatg
     9721 ccgcgcatcg cgcgattgat cgcgaacacc tgccggtgct cgccgacgat catcacgccg
     9781 tcgggtagct ggtcgacgat gctctgcagc caggcgtagc tgcgcgcggt ttcgatgacg
     9841 agcgccacac catctgcgag caggccagcg agccgttcgt cgcggtcggt gaattcggcg
     9901 ctgtcacgct tgttcgcgag gaagatgttg ccgaccacct cgccacgctg ccggatcgag
     9961 acggccagaa agctatgcag ctcgggatgg ttcggtggca ggccgatgaa tgcgggatgc
    10021 tccgtgattt cgcgcacgcg cagcggctcg aggctccggc tggcatgcgc gagcatgccg
    10081 acggagcgtg gcggccgacc gatcgctgct tgggtctcgg ccgacatgcc cgatgtcgcc
    10141 cagtgttcga atgggcgcga ctcgtcgccg ccgatgccaa cggcggcata cgtcgcattg
    10201 gtgagcgcgc gcgcctgctc cgcgatctgc gcgagcacag ggtcgatgtc tgcgccgcgc
    10261 agctctgcgg ctgcctcggt gacgacttcg cgtgcacgat cgaccgcttc gaggtctgca
    10321 cgtagcgcgc gctcggctgc aagcgcgtcg gcgagcagtc gcgcgtggcg cttgcgctcg
    10381 gtgatgtcgc gcacgaatgc ctgccaacgc cgatccggaa gcacgttggc aacgacttcg
    10441 acttcgatca tcgacccatc ggcacgccgc aaccgccatt cgcggcgcga cgtcttgccg
    10501 gcaatgatgt tcgcacgctc ttcggcgagc ttcgaccgtt cctcaggtgc ggcaaggtca
    10561 gcgatcgtca tgcccagcag ctgctcgcgc gtgtagcccg acagctcgca cgcggcggga
    10621 ttgacctcga tgtacttgcc gtcgaggtcc gccaggaaga tgccgtcctt tgcttcctcg
    10681 acgagtagcc gtacgcgagc ctcgctgcgc tgtagatctt cggtgagctc gcgttgcacc
    10741 gcgtcgatca tgctgagccg ccgtgcggtg ttgagaatga acacgctcga ctcggcgagc
    10801 gcgaacaaca gcgtcagcgc ggcggcgagc ggcagcgcgt agagctgaaa ccgggcgccg
    10861 aggatcagga tcgccgcgac cggtggaaac agcacgaggc cgaccagcag cctacgcgcg
    10921 acgacgccac cggcatcggg tgcaagcacg atcgagatca agccgtcctc gacgtgcaga
    10981 ccaaggatgc cgatcgacac cgccaacgag atcaacaccg tcgtcggact catgcccacg
    11041 tagggcacca cgtgcgcctc gacgccgaac aagaacccga gcagcgccgc catcgcgaat
    11101 gcagcggctg caaccgcgag gtaatcagcg aagtagcgac cgcgacgcga cttgcgctca
    11161 ccgaggagaa gcgccgagga gaccagcaag tacgccgcga ccgtgtacgg cgagggcctg
    11221 cccaccggcc acggcgcgtg ctcgacatca gcgagccagt gatcgagacc caggtcgacg
    11281 cccgttacgt attcgacaag tgtggcgagc aacatgagcg cgaggatcgc ggcgaccgta
    11341 cggcccgcta cgcgcgcgcc acgtccccac tgcgcactcg ccaggagaaa cagcgagacg
    11401 ccggcgagca cgcccatgaa tgcactgttc ggaaccgtga cgggcgacgg cgcactgagg
    11461 cgcgtgatcg caacgatgtt cagcagcccc ccgagcagga cgatgaggcc gaatattgag
    11521 gcggccacag cggccgcccg cgggtagtag tgaaagaagc tctgaagccg ccgcggcgcg
    11581 cgcggcagga ccgcggtgtg ggccgcagcg gtcgccatag gggcagaggc agcaagccgc
    11641 gaaccaatga gtgggatccc gtcaaagtaa gtgtttgatt gcagtggggt tgaggcccct
    11701 tcgttgtggt tgacaaccac atcgcctcac actaggttcc gcgcgccctt cagccggggc
    11761 gccttttggg gacacgtgga tagatatgga ttctgcaaag gacgtgggcg ctaatcgcgc
    11821 aaagcggacg gatatggcgc ctctaacctg ggatcgaaac acaccgaccc cggggtcctc
    11881 gctcgatacg gatgacgctc ctgacggtac ggagcagacg ccggtgttcg agaccgcgga
    11941 tctcggtgag ggcttcggtg agagtgaagg cgagcgcaca gtcattacgg ctgcgacgcc
    12001 gtacgacctg cgcccgaagt tcaccggtcg caacgctgcg ctcgcgcagt tgcagacgct
    12061 caccgacaag gcgttcgccg aaaagcaaat cgcattcgct gtcgtggtcg gcgagcccgg
    12121 gatgggcaag agccgcatcg tcagcgagct gatcgcacgc acgaaaacga agattccgaa
    12181 catccacgta ttgcagggtg tggcggacga gaacgcgcac gcgtacggac cgatcgcacg
    12241 tgcgatgacc gtgcggttcg gccttcagcc gggtgaggat ccggccgaga gccgcgacaa
    12301 gatccaggcg accgtcgccg aagtcgtgca ggcatcgcgc gtcaccgaga tctcgcactt
    12361 gctcgcgcac ctgttgcgcg tcccgttcga cgacagcccg gtaatcacgc cgcttctcga
    12421 gtcgccgcag cgcctcgagg cgcgtctgtt catggcgctc aagcgcctgc tcaccgcgga
    12481 ggccgagaag cgcccggtcg tgatcgtcgt cgagaacctc gagctgtgcg gcggggacac
    12541 gatcaacttc ctgcagtacc tcgcggccgg catgaccgac cagcgcgttg cagtcctcgg
    12601 cacggcaacg ccggcgctgt gggaacgcca cgccgcgttc ggcgaaggcg aagtcgcgcc
    12661 gatcaagatc gagctcggcg cgctgacgcc gaacgagtcg gagcagctgc tccgcgagct
    12721 gtgcaagcag ctctcggaag tccccgcgcg actgatcggc cacgtgcgca acctcggggg
    12781 ctcgccgcgc gcgatccac
//
LOCUS       DASUFX010000510        25127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_636053,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000510 DASUFX010000000
VERSION     DASUFX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25127
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636053"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFV99_20385"
     CDS             <1..844
                     /locus_tag="VFV99_20385"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5061742.1"
                     /translation="VAAACGERLIPCTLELGGKAPAIVCGDADLDRAAQAIVWGGFAN
                     SGQVCASVERVYAVDTIHDALVSRIVRHARELRQGDASASQDVDVGAMSWDQQVTHVE
                     KLVDAAVAAGAKAETGGRRKTGAGLFYEPTVLTGCTQAMDVMRKETFGPVIPIMRVHT
                     EDEAVKLANDSNLGLLAYVFTRDRERGKRLAERIEAGTVMVNDVLNTYACPETPWGGV
                     KQSGIGRTHSMIGLRDLCDTRHVNHDRVALRREVWWYPYKEGTFRALLRGARLLFGKR
                     PWQR"
     gene            complement(841..1284)
                     /locus_tag="VFV99_20390"
     CDS             complement(841..1284)
                     /locus_tag="VFV99_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061743.1"
                     /translation="MAVDLMRAGFAIENEWYWKITEGAQTFDALCAVRDFLAPLARYA
                     KGKIAITDIASEVDEVRFALGDTTHSIKLYAGVADRIALNHFVSDVDRALALANTGYQ
                     FALVAPKRYELRGVLLTDAEWQALAGNAAVLVPTTRASWQKIPPS"
     gene            complement(1322..1669)
                     /locus_tag="VFV99_20395"
     CDS             complement(1322..1669)
                     /locus_tag="VFV99_20395"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5061744.1"
                     /translation="MTKVDKPWGHELIWAKTDRYVGKILHIKAGEALSLQYHRVKDET
                     IMVLTGRLRFEHFADGEPPRTAELGPREPFHVTPGLRHRMIAIEDTDVLEVSTAELDD
                     VVRLEDRYGRAKP"
     gene            complement(1666..2382)
                     /locus_tag="VFV99_20400"
     CDS             complement(1666..2382)
                     /locus_tag="VFV99_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061745.1"
                     /translation="MMGKLWKLVAIGILLAASGCKSKTPDTTPKNTASLEVPKVDPTL
                     CETAGKNVVTSDLNKDNKPDVWRLYKTEDEGGTKIEFLTCKQVDMNQDGKKDWVVAYN
                     RKGNPIFEKTDYNYDGKWDMSAVFDQKTGHYAEIERDDDHDGRFEKKELYDTAGVLTS
                     VRRDRNGDNQPDEWEQYKDTILIAILYDDDYDGRVDRREEIPGATPKVEMPTTMDPTA
                     SGNVPPDKTPAKTPAKSTKK"
     gene            2459..2644
                     /locus_tag="VFV99_20405"
     CDS             2459..2644
                     /locus_tag="VFV99_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX5061746.1"
                     /translation="MARKKISTTVYITPEQDARLKALHNRTKVPVAEYIRQGIDLVLE
                     KYRDQLPGQVDLHLGSR"
     gene            2664..4466
                     /gene="lepA"
                     /locus_tag="VFV99_20410"
     CDS             2664..4466
                     /gene="lepA"
                     /locus_tag="VFV99_20410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5061747.1"
                     /translation="MSFPPERIRNFSIIAHIDHGKTTLADRILEECGAVTAREAQAQM
                     LDSMDLERERGITIKAQAVQLHFKAKDGQTYQFHLIDTPGHVDFHYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYVALDNNLEIVPVLNKIDLPVARPDEIKQQIEDTIGLDAS
                     QACLVSAKTGVGVHDMLEAVVERIPPPKADREAPLSALIFDSWFDQYRGVVVLVRVMS
                     GTIKKGTKLRFWATEQTYECNMIAVRRPAPFEIDELEAGQVGILAAAIKDIAHARVGD
                     TITDADRPIDAPLPGFKTVKPMVFAGLFPVDAADYQDLKDALGKLVLNDASVTYEPET
                     SSALGFGFRTGFLGLLHMEIIQERLEREFNLNLITTAPSVRYRVKQQNGEIIELDNPA
                     KVPDQGKYEAIEEPIINATVHLPQEYVGPIMQLCQERRGVQQGLHYDPGGRVRVVYEM
                     PLAEVVFGFYDRLKTMSRGYASLDYEPAGYREADLIRLDVLVNGERVDALSIVSHRDS
                     AYHRGVELTTKMKDVVPRQMFDVAIQAAIGSRVIARTTVKAARKDVTAKCYGGDITRK
                     KKLLEKQKEGKKRMKAVGSVELPQEAFLTILKVD"
     gene            4466..5581
                     /gene="lepB"
                     /locus_tag="VFV99_20415"
     CDS             4466..5581
                     /gene="lepB"
                     /locus_tag="VFV99_20415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5061748.1"
                     /translation="MRAASLDRRVRKEATLLVREARAALGVKPGLRGKGGDLATVTTD
                     VEKGLRESDMKRVRYHLPILDALVDELVKRPPKSTTRDYVESIGAAILIALALRAFVI
                     EAFKIPSSSMYPTLEIGDHIFVNKFIYGVRIPFTKTKLFELRGPKRGEVIVFIYPCDP
                     ERDYIKRVIATAGDTVEVRCNIVYVNGIPVPSKMIESGETCSYKDFDEHDNRWFTRQC
                     SRYRETVNGVDYDTFHDPERPARDARIAEGNPPPGGDARDFPTRGNPFPPSCSQSDEA
                     LKAANIVQVKGKIVETKPEAVAKVCEPQLHYIVPPGHVFVQGDNRNNSNDSRVWGAVP
                     IENIKGKALFIWLSYEHWGPLDWTGIRWDRIGNFVHH"
     gene            5667..5978
                     /locus_tag="VFV99_20420"
     CDS             5667..5978
                     /locus_tag="VFV99_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061749.1"
                     /translation="MSRFAVLAAIAACGGSTGRTGLDGRIACGDKFCETGQLCATTEA
                     GVDAAVPGQPIFYCVTVADGCFVEDCLNSACPVCIRDLCSPGTAYGVELDGRNLTCAG
                     V"
     gene            complement(5988..6569)
                     /locus_tag="VFV99_20425"
     CDS             complement(5988..6569)
                     /locus_tag="VFV99_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061750.1"
                     /translation="MKSLLLVVCALVVLAPRADAAPPKKKYYFELTKVLVKPEVKPDV
                     AKEAQPRIEAVFKKALESHPQLVKLEGAPADPEANNGTTYRKFLTKKGVAAAYLVTVE
                     VTEASIEVVPLEDKKNAQRISVTVGLHVLGETIPGRTMGFTGDGRATVKQEVGMKIRD
                     KDRQFTWDSAAETAVENALKECFAKLALPAKKQ"
     gene            6616..6783
                     /locus_tag="VFV99_20430"
     CDS             6616..6783
                     /locus_tag="VFV99_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061751.1"
                     /translation="MVRPRWHDALIVSAIVALLAVGVWALWWEDVRNLLHLGPGEAAS
                     PPPAAPANPQT"
     gene            6892..9165
                     /locus_tag="VFV99_20435"
     CDS             6892..9165
                     /locus_tag="VFV99_20435"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5061752.1"
                     /translation="MFTIIIQEKGGEQRRMVFNKPEVTIGRVQGNDIVLPKGNVSKRH
                     ARIVLKDGKFIIVDLKSTNGTYVNGRKITSPLVVKDSDKIYIGDFIVGVDEAANAEGD
                     GPSETTTSPPGADRGGPIVPDARPPRPTEAAPQPMPIPMGGPMGGGMSGGMGGGMGGG
                     MGGMDGSDRMLPERPAAAGPSQSVPPPMARPAGGPPRPPGALGGPPREPPGPREPREP
                     LGGPRGGDMGGPGREMPPVAPPPSNLRPGARPGGTMPPPVAPLGSPPAPVPPPPAMGD
                     FPPPVAAPAPSPMSAAPAIAPAPLPSAQPAPVVPLKNEQRARAAQPQPGPSRKVSRSV
                     SPVAKRGVALEPLDSKIVKMLDLQSNILERLRAKLDLDKVPIERLHEEELWQKAERAT
                     IDLVETLETSGELPKYIDQDALIKETLNEALALGPLEDLFADEGIDEILIDRRDRVVV
                     GKNGQLRGSGKAFSSDEVFERVVKRLVHEAGAVIDESRPVVDMRMRDGTRLTAAVSPV
                     AARGACLVLKKPASATPTLSELVSAGTLSSGMSDFLTTCINARRNILVCGAPGSGKTT
                     ILGALAAASPAGERVVSIENVAELAITRDEWIQLETRPGSGKASDIDLGALLDTAMRL
                     MPDRLVVGDVRGREAMRLIEALSSSVDGAVVGVTGEGANSVLNRIATLARVTQPWGSD
                     AAVRELVATAFEIVVHVGRTADGALKVHSIEEVTGVSDTHFETELVFCFKDGSFAASG
                     KVPSFYSAMGADQAVFR"
     gene            9266..9769
                     /locus_tag="VFV99_20440"
     CDS             9266..9769
                     /locus_tag="VFV99_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061753.1"
                     /translation="MEPRVRFIVDLSSDLASLDTFPLRWRWTEQRHTLLSPHELARVR
                     PLSTQKARDAWDLSLSWHAREMDQATPSEKLFIVETLDRPHERDGTGWLRARLPSEPV
                     PVIVSWQPDWAVLTDASLFVERWETFCYPASDDVDIWPLDGRWLAVYWHEEQLAYGAR
                     RADEIRT"
     gene            complement(9805..10752)
                     /locus_tag="VFV99_20445"
     CDS             complement(9805..10752)
                     /locus_tag="VFV99_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061754.1"
                     /translation="MAKKRDDGEPLDRIFAKLEDAPPGLHDVDPPAEELPSGLPAALI
                     ELYARCDGARIFVESVEILGSHAVQYENGRWLFASALGDAISVDEKGKVWRNDESIED
                     EVCDGTRIDRWLAGQLDALDLIFDHDGEYADDVFDDDGEILPIVREKQLRAMIKRDTA
                     APAPRWRLAHVLIEQDAMEDARNELEQVVADDPVFAWAWLDLARVSERLGESTGAIDE
                     ARMAAEAAEGVQHPQAGYFWSQVARLASRAGDEMVRAEAATKASLLAPNLKRAQLDGA
                     RERIDAGDKASAKGLLDLLRAVWPRDLEVLELARRVEST"
     gene            complement(10762..12138)
                     /locus_tag="VFV99_20450"
     CDS             complement(10762..12138)
                     /locus_tag="VFV99_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061755.1"
                     /translation="MKRVAAALSLVLFASSAHAWESQTTQAGLAEQAAMSSRLNKRLV
                     SLGFTGGLFEPLTIPPADAPALMKDLKLLSPSHGSVPDSRGRQTALAWIAAGAALADV
                     PSRHAVNHFYDPATHKGWERPERGVFKGLSDSVREAIGRVTLPAHGMPAPDWITSKDN
                     PFHLGAFMDQYAKAVSAATPGERSRHMAAALIAAGAIIHVLGDLGSPSRVRGDEAAHF
                     EPLGGGPDDLGSRFERIAALAYGRLGVPAPSRVITRDHMHDFFSATDGGGLADMVSRS
                     YFSPNTLPQSTRVGDETKPKLARPQPALPSRLNLMAASREDGTTLRNQAGTCLARYKV
                     DHGVLSFSLDDDCMLEQVAVLLPEVAAYETGLLDFLLRGELTITGGQQVVVSGKGLGA
                     GSLDVLVEDDRGVRTSIGTAQTAGGKDELVRLAAPGAGTRIVAVFRGADAQGEPIVAV
                     GALPLTSQ"
     gene            complement(12135..13175)
                     /locus_tag="VFV99_20455"
     CDS             complement(12135..13175)
                     /locus_tag="VFV99_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061756.1"
                     /translation="MLGKVVLLLALVQAACVESSIRTAPFRSRPDAADPGSLRGPFTG
                     RVIDATTKTPIAGALVYAAWTLERGTALTEPAGSREVVGSTDAGGNYKIAALGSLPAH
                     TRVTDFTLLVYKRGYIAYRSDRRFHDLGARMDFAQNDNQVLLERWRNELSHARHLRFV
                     GSGTAVAALTQWELADASAELEGKRPSSEDLRPGREGNYVVAAQLLSDTDIKNRTKYE
                     GQFETGPLSDEPDTATYSSQHFKALARPETWDVAIRLWRLEPAKAQERYEELLTQLPG
                     VQEKDDIASRSFIANENDIRGVGFLDGTRGVVVLLTCGVNQCTTLDDATALASGVYNR
                     LKQLTPATGGNK"
     gene            13299..14675
                     /locus_tag="VFV99_20460"
     CDS             13299..14675
                     /locus_tag="VFV99_20460"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5061757.1"
                     /translation="MLRCSACGHQSPPGSSFCLNCGTALASAPAQTPGGGAGLPVICS
                     ACRGENPPGMKFCRNCGAALSGTTPGAPAFGAPMMAPPGPPPGPPLPGMPPAGPFATP
                     SPISPSAKGMTPPGAPLGSAPLAGPSATPLRVSAQGSTITCPRCGTSTPTGFAYCQQC
                     GLHLNAIAPTDPGAGAARPRPITNAPPVPLNAPIDPHAGTLAAPDGAAIRAGAISAPA
                     RSSSAAAPAAPPVVVLPQAPTRPSAGSWGTAVLVNRDGSDGQRFPLASDDTIVGRAGA
                     DIAFDDDRFLARQHARIERTSDGNIKVHPLDATNGVFKKTDGSVELADGQIILVGREV
                     LRFERVAQEERTVHPLVRHGVALFGSPPRDPWGRIVQLIPSGGYRDVRHLIGEEIVLG
                     REEGDIVFRDDAFMSRRHAAITWDGRRALLTDLGSSNGTFVRLSGPTSIKSGDHVRMG
                     DQLLRIER"
     gene            14672..15811
                     /locus_tag="VFV99_20465"
     CDS             14672..15811
                     /locus_tag="VFV99_20465"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5061758.1"
                     /translation="MTDTPRLQITIGAASDVGQVREHNEDAYLVGNLDNATVIELGEP
                     WEASLDRGPVLVVCDGMGGVEGGEVASDLAAKVIWREMKGTGATRDPEVYARLLRRAT
                     RVANHDVHAMSKREPGLRGMGTTVSAAGIAGDRLIVATVGDSRAYVLRSGEIVQVTRD
                     QSLSSALLAAGHDAEQAERAGSAILQALGVGDDVEPSLSIVELRRGDRVLLCSDGLHG
                     LVGDPALRLLMSEPHTVAEAVSLLVTAARAAGGSDNITAVVAHVGGDRLVPPKGDDDL
                     PTFREFDPQKEGDPALTSTSYVARRLAAKIGLPTSSIPPSIPATGQHAILRSRVGLRV
                     PATALPIDDISGPARQRLRASALGWWWYVLIAAIACVIAWVAGGR"
     gene            15858..16322
                     /locus_tag="VFV99_20470"
     CDS             15858..16322
                     /locus_tag="VFV99_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5061759.1"
                     /translation="MRVVIDHIAGSRRGQRQEFPTSSRIRFGRHPDCEVSFDPHRDID
                     ASSRHAELRVIETPSATESVSQAWVLVDLGSSNGTYVEGHRVTETPVTRNVPVSIEFG
                     PGGPRIRLLVGDDAAIEGLPPVQVERAKRRWVLPVVAGAIVLIAVSFLLMQC"
     gene            16382..17935
                     /locus_tag="VFV99_20475"
     CDS             16382..17935
                     /locus_tag="VFV99_20475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061760.1"
                     /translation="MNCPSCSSPLEPTARFCGVCGYRLQAARPPGASTPATASPQARP
                     ASNPVIPQRTATPPAGQPLKQQQAPQKTIARAAPRGRNRDDIYLEAVLNNRFKVESKI
                     GEGGFGAVYRGVQLATGRKVALKLLHPEMTKDENLVARFRREGMVLCNLRDAHTITTY
                     DFDQTPDGTLYIAMELLEGKSLHQVFHEQAPLEWKRMFKILIEMCSSLAEAHQQGIVH
                     RDLKPENVYLESRPGNPEFVKILDFGIAKVMRGDSIDPQSPQLTATGQTLGTLEYMSP
                     EQLMGKQLDGRSDVYALGVVAYEMITGRLPFPDAKGPAGLITAQLKQTPQPPSQAHPK
                     ANLPPQADRTILKCLEKDKNNRYADVTQFSHALQEVLDAARDQSNPLATPLPMAAQSS
                     SSRASPPPAGMDLLETRRGEMPNMAPPPMPSAGASVPAMQRGVTPGVGTPMTPMMNPH
                     VPATPYPQAPANHDHQLQYPQAQPHMYPAGAQTEVNPTQGSKKWIWWIVALLALGAGA
                     GAVLALVMQ"
     gene            complement(17937..19640)
                     /locus_tag="VFV99_20480"
     CDS             complement(17937..19640)
                     /locus_tag="VFV99_20480"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HEX5061761.1"
                     /translation="MPDLVIGGRRSAFPRPWRAAGIAFALLGVVLVVAWFIYRRSVAY
                     DVPDGDVHGEIASTQVAPGAPPILTFGNASLEWLGGIAVLRAQGESHAIGAAHGRLLA
                     PLIQPAIRAARPSIEATVSDEGWFGGLTHNMRLAWQWRFIDDGLSDADRRIIAGITRG
                     AGASGVGVGFAAVLRSQAVLDVGAPSRRSGEAERHSLAHSLTVLGQQAQMPARVWIGR
                     TFSLSGMDDGGDSAVPVVSIVKPTGKFAWASVGWPGQAGVVTGINEHGIAVMVDPART
                     GDVRPTRSARPVALLARAILEQAKTLDEAVKMVEQSPTLGAALLVIVDGGSGKWVIVE
                     RTPSKAIVERSPKTNAFGDVLTTNALSSDPENDRARRVLATIARVERAAKLVKAPLAD
                     VTAMATVLRDQRGTDDSPRPAGHRGVIDDARARHVVILDPASLQLWVADPNANGRMRA
                     FDLRHELRNEGDRAVPPADIPADPAAEPDRVANVTAARADLRIARDALARGDHRRASE
                     ACARARARAADLPEAIELEAVIAQQRGDDARAKKLYQQWLDGGADDPVAEERARALLN
                     R"
     gene            19932..20540
                     /locus_tag="VFV99_20485"
     CDS             19932..20540
                     /locus_tag="VFV99_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061762.1"
                     /translation="MTLAATEPLLIVVVALLVLVIAGLIAMALFVRRRSRPQPQPAPE
                     PPRPQPLPDRPPPRPRPRPAMVRGGGGGGLAVATSSAMVCPTCRTEYHGMAYCTRDAR
                     RLVPPEDMLGGPLRAMSSGKSAGLVCMACRRAYEPGLRSCPHDGNELVPFPVYNATRP
                     RSRRNSTEPAGVVARICPVCGTKYDLNARFCGHDAGELVVIN"
     gene            complement(20617..22152)
                     /locus_tag="VFV99_20490"
     CDS             complement(20617..22152)
                     /locus_tag="VFV99_20490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5061763.1"
                     /translation="MTLLYDWLKKAAKVQGNNKAVVYRDNYLSWRGLLHRVDRRAQEF
                     KSMGIKEGAWVGLMLGNVPDFVILALALSKLDAAIVPIDPTTGGRELELILAAAPLRA
                     LVTRPRGSEGSISANGTSAPVPPSTLPRSRVQRPTTPPGQPPPAPAADAAEVRRRLQG
                     TLLTCSVYKRNPPDHGVDPIAVLFTADSLGDPKGVLRTDKNTTAAVDHAIAALGMNDK
                     TKVLVAVPLFHAYGWDLGFLPTLKLGCTMFLEEEISARRIGKLVREHEVDVLPGTPSM
                     YAELSKLPTVKKLELEKPKYLAAGSRLDQTVADSFLNEYGVRILSCYHSTEAATVALE
                     DTGKFPTTVGKPIEGVDVKITSPDGKAATGGKEGLIWVKSKTLSPKSIGPFDEEKPAA
                     TRASGMVAIGSIDKGGWLRTGDLGKLDKNGRLCITGREDDVVKVDGKRVALGEVEGCL
                     EAMPKIKAAQATVVTDPMAGAMVVARVVTKQKCGAEEIIDHCARNLAPYKVPRRIEFV
                     ETI"
     gene            22307..23461
                     /locus_tag="VFV99_20495"
     CDS             22307..23461
                     /locus_tag="VFV99_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061764.1"
                     /translation="MSAAQRPQTEDSPESADAEGWALLDGLRRRLDEQANQNRKTQSE
                     VRQLAESIGAMVEQQRKRSRWLNVNSFVAYVMFTLLCGGAFYFVYESRARELVAERER
                     VKTERDTAVQRANAAEKKTAAREAADTKAWDAYQLLEAGKREDAAKKLVELQGAPLSK
                     FERTVLETKAKQAEVVQIDLALKGAAAAFKAGRHAEVVAPLEAALALDPNGPRAPLMH
                     YYLGVAASKAGALDAATAHLQAAVDGNVDLEDARFHLASVLDRAGQWGKAKIEYDRFA
                     TAHPMSSYTWYATRRGGALSRMPAVRPPNVTQEMAATQQSALDHAGTTLPPSAKAPAT
                     AEPPAPPTKAAPKPAAPKAVAPKPAAPNQPAPKTLDFTNDNPAADGSASQ"
     gene            23458..24066
                     /locus_tag="VFV99_20500"
     CDS             23458..24066
                     /locus_tag="VFV99_20500"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5061765.1"
                     /translation="MTALLTPPAQQYSASIHKLSADVCARITNKSWRDDPRCPSVADL
                     AYLEIAHVTFDGGVARGELVVAAALAKRAVELFRRLYQLGFPIRQMQLVDDYDASDDA
                     SMAADNSSAFNFRVIAGTKLLSQHALGRAIDINPVENPWRKPDRIVPPAGERFADRKN
                     VRPGMIVRPGPIVAVLDELGWEWGGDWRHAFDDHHIVWSRPL"
     gene            24063..24422
                     /locus_tag="VFV99_20505"
     CDS             24063..24422
                     /locus_tag="VFV99_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061766.1"
                     /translation="MKTLAMLLVLVCACKDSPCDKYADRLLACNPKLPGDVRENVYGY
                     CVAATIHEPEPNAEPNRGDVLMYRAVKACSPTLACTDFLACLQEQNCTLITSINTPGE
                     LTFQCWPTPPPPAKLPQ"
     gene            complement(24416..>25127)
                     /gene="lepB"
                     /locus_tag="VFV99_20510"
     CDS             complement(24416..>25127)
                     /gene="lepB"
                     /locus_tag="VFV99_20510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5061767.1"
                     /translation="SMYPTIEIGDHIFVDKLSLYWRAPERGEVIVFEYPCTHDIDYVK
                     RVIAVGGDTVEVRCNVVYVDGVAIPNELVVASDQYDDYDPEEHRWYTRATSRYRERIG
                     SQTYEVFHDPDRPERDRHRDTLPAGDARDFPNMEKLIAPSCTQGEFYNRPTPSAGQLT
                     GKIVVNVHDAKPCEQQAHFVVPDGGLFVMGDNRNNANDSRYWGVVSVDAVRGRVLGVW
                     INDPPGGDRKWSRVGAIH"
BASE COUNT         4126 a         8431 c         8352 g         4218 t
ORIGIN      
        1 ggtcgcggcg gcatgcggcg agcgcttgat cccgtgcacg ctcgagctcg gcggcaaggc
       61 gcccgcgatc gtgtgcggcg acgccgacct cgatcgcgct gcacaggcga tcgtgtgggg
      121 cggctttgcg aactccggcc aggtctgcgc atcggtcgag cgcgtatacg cggtcgatac
      181 gatccacgac gcgctcgtca gtcgcatcgt gcgtcacgcg cgcgagctgc gccaaggcga
      241 tgcatcggca tcacaggacg tcgacgtcgg cgcgatgtcg tgggatcagc aggtcaccca
      301 cgtcgagaag ctcgtcgacg cggcagtcgc tgctggtgcg aaggcggaga ccggcggccg
      361 tcgcaagacc ggcgccgggc tgttctacga gccgaccgtg ctcaccggct gcacccaagc
      421 catggacgtc atgcgcaagg aaaccttcgg tccggtgatt ccgatcatgc gcgtccacac
      481 cgaagacgaa gcggtgaagc tcgcgaatga ctccaacctc gggttactcg cgtacgtgtt
      541 cacgcgcgat cgcgaacggg gcaaacgact cgccgagcgc atcgaggccg gcaccgtgat
      601 ggtcaacgac gtgctcaaca cctatgcgtg tccggagacg ccgtggggcg gcgtcaaaca
      661 aagcggcatc ggccgcacgc actcgatgat cgggctgcgc gacctctgcg atacccgcca
      721 cgtcaaccac gatcgcgtcg cgctccgtcg cgaggtgtgg tggtacccgt acaaagaggg
      781 cacgttccgc gcgttgctcc gcggcgcgcg cttgctgttc ggcaagcggc cctggcagcg
      841 ctaacttggc ggaatcttct gccacgacgc gcgcgtcgtc ggcacgagca ccgcggcgtt
      901 gccagcaagc gcctgccatt cggcgtcggt gagcagcacg ccgcgcagct cgtagcgctt
      961 cggcgcgacg agcgcgaact gatagcccgt gttcgccagc gcgagcgccc ggtcgacatc
     1021 gctcacgaag tggttgagcg cgatccgatc tgcgacgccg gcgtagagct tgatcgaatg
     1081 ggtcgtgtcg ccaagcgcga accgaacctc gtcgacctcg ctcgcgatgt cggtgatcgc
     1141 gatcttgcct ttggcgtaac gcgcgagcgg cgcgaggaag tcgcgcacgg cacagagcgc
     1201 gtcgaacgtc tgcgcgcctt ccgtgatctt ccagtaccac tcgttctcga tcgcaaaccc
     1261 ggcgcgcatc agatccacag ccatcgcgcg gccgaacgta gtccggagcg gtccggagca
     1321 gttacggctt tgcgcgtccg taacgatctt cgaggcgaac gacgtcatcg agctcggcgg
     1381 tcgagacctc gagcacgtcg gtgtcctcga tcgcgatcat ccggtggcgc aatccgggcg
     1441 tgacgtggaa cggctcgcgc ggcccgagct cggcggtgcg tggcggctcg ccgtcggcga
     1501 agtgctcgaa ccggagcctt ccggtgagca ccatgatcgt ctcgtctttg acgcggtggt
     1561 actgcagcga cagcgcctcg ccggccttga tgtggaggat cttgccgacg tagcgatcgg
     1621 tcttcgccca gatcagctcg tgaccccagg gcttgtcgac cttggtcact tcttcgtgct
     1681 cttcgccggc gtcttggccg gcgtcttgtc gggcgggacg ttgccggacg ccgtcggatc
     1741 catcgtggtc ggcatctcga ccttcggcgt cgcgccgggg atctcttcgc gccgatcgac
     1801 gcgaccgtcg tagtcgtcgt cgtagaggat cgcgatcagg atcgtgtcct tgtactgctc
     1861 ccactcgtcg ggctgattgt cgccgttgcg atcgcggcgc acgctcgtca gcacaccggc
     1921 ggtgtcgtac agctctttct tctcgaagcg gccgtcgtgg tcgtcgtcgc gctcgatctc
     1981 ggcgtagtgc cccgtcttct gatcgaagac cgcagacatg tcccatttgc cgtcgtagtt
     2041 gtagtcggtc ttctcgaaga tcggattgcc cttgcggttg taggcgacga cccaatcctt
     2101 cttaccgtcc tggttcatgt cgacctgctt gcaggtcaga aactcgatct tcgtgccgcc
     2161 ttcgtcctcg gtcttgtaga gacgccagac gtcgggcttg ttgtccttgt tgagatcgct
     2221 cgtgactacg ttctttcccg cggtctcgca cagcgtcggg tcgaccttcg ggacctcgag
     2281 cgacgcggtg tttttcgggg tcgtgtccgg cgtcttgctt ttgcagccgg aggcggccag
     2341 caggatgccg atcgcgacga gtttccagag ctttcccatc acgactggag cctagtcgct
     2401 gcgcctaggc ctagcaacca ttgaccatgt gcatatggtc acgtagggtc actcgacctt
     2461 ggcgcgcaag aagatctcga cgaccgtgta catcacgccc gagcaggacg cgcggctcaa
     2521 ggccctgcac aaccgcacca aagtaccggt cgccgagtac atccggcagg gcatcgatct
     2581 ggtgctcgag aagtaccgcg atcagctccc cggccaggta gacctccacc tcggctcacg
     2641 ctaactaacc gcctggcctg tcgatgtcgt tccctcccga gcggatccgt aatttctcga
     2701 tcatcgcgca catcgatcac ggcaagacca cgcttgccga tcgaatcctc gaagagtgcg
     2761 gcgcggtgac cgcgcgcgaa gcgcaggccc agatgctcga ctcgatggac ctcgagcgcg
     2821 agcgcggcat cacgatcaag gcgcaggcgg tccagctcca cttcaaggcg aaggacggcc
     2881 agacgtatca gttccacctg atcgacacgc cgggccatgt cgacttccac tacgaggtgt
     2941 cgcgctcgct cgccgcgtgc gaaggcgcgc tgctcgttgt cgatgccgcg caaggcgtcg
     3001 aggcgcagac gctcgcgaac gtctacgtcg cgctcgataa caacctcgag atcgtgcccg
     3061 tcctcaacaa gatcgatctg ccggtcgcgc gccccgacga gatcaagcag cagatcgagg
     3121 acacgattgg tctcgacgcc tcgcaggcat gcctggtgtc ggcgaagacc ggtgtcggcg
     3181 tgcacgacat gctcgaggct gtcgtcgagc gtatcccacc gccgaaggcc gatcgcgagg
     3241 cgccgctgtc ggcactgatc ttcgacagct ggttcgatca gtaccgcggc gtcgtcgtgc
     3301 tcgtgcgcgt catgtccggc acgatcaaga agggcacgaa gcttcgcttc tgggcgaccg
     3361 aacagacgta cgagtgcaac atgatcgcgg tccgcaggcc cgcgccgttc gagatcgacg
     3421 agctcgaagc agggcaggtc ggcatcctcg ccgctgcgat caaggacatc gcgcacgcgc
     3481 gcgtcggcga cacgatcacc gacgctgatc gcccgatcga cgcaccgctg cccggtttca
     3541 agaccgtcaa gccgatggtg ttcgccggtc tattccccgt cgacgccgcc gattaccaag
     3601 atctgaagga cgcgctcggc aagctggtgc tcaacgacgc gtcggtcacg tacgagcccg
     3661 agacatcgtc ggcactcggc ttcggtttcc gcaccggctt cctcggtctg ctccacatgg
     3721 agatcatcca ggagcgcctc gagcgcgagt tcaacctcaa cctcatcacg accgcgccga
     3781 gcgtgcggta ccgcgtgaag cagcagaacg gcgagatcat cgagctcgat aacccggcca
     3841 aggtccctga ccagggcaag tacgaggcga tcgaggagcc gatcatcaac gcgaccgtcc
     3901 acctaccgca ggagtacgta ggtccgatca tgcagctgtg ccaggagcgc cgcggcgtgc
     3961 agcagggcct ccattacgat ccgggtgggc gcgtccgcgt ggtctacgag atgccactgg
     4021 ccgaggtcgt gttcgggttc tacgatcgac tcaagaccat gtcgcgcggc tatgcgtccc
     4081 tcgactacga gccggccggc tatcgcgagg cggatctcat ccggctcgac gtgctcgtca
     4141 acggcgagcg cgtcgatgca ctgtcgatcg tctcgcaccg cgacagcgcg tatcaccgcg
     4201 gcgtcgagct gaccacgaag atgaaggatg tcgtgccgcg ccagatgttc gacgtcgcca
     4261 tccaggcagc gattggcagt cgcgtgatcg cacgcaccac cgtcaaggcc gcacgtaagg
     4321 acgtcaccgc aaaatgctac ggcggcgaca tcacgcgtaa gaagaagctt ctcgagaaac
     4381 agaaagaggg caagaagcgc atgaaggcgg taggctcggt cgagcttccg caagaagcgt
     4441 tcttgaccat cctcaaagtc gactgatgcg cgcagctagc ctcgatcgtc gcgttcgtaa
     4501 ggaagccacg cttctggttc gcgaagcgcg tgcggcgctc ggcgtgaagc ccggcctacg
     4561 cggcaagggc ggcgatctcg cgaccgtgac cacggacgtc gagaaggggc tgcgcgaaag
     4621 cgacatgaag cgggtgcgtt accacttgcc gatcctcgac gcgcttgtcg acgagctggt
     4681 caagcggccg ccgaaatcga cgacacgcga ttacgtcgag tcgatcggcg cggccatcct
     4741 gatcgcgctc gcactgcgcg cgttcgtgat cgaggcattc aagattccgt cgagctcgat
     4801 gtatccgacg ctcgaaatcg gcgaccacat cttcgtcaac aagttcatct atggcgttcg
     4861 cattccgttc acgaagacga agctgttcga gctgcgcggt cccaagcgcg gcgaagtgat
     4921 cgtgttcatc tatccgtgcg accccgagcg cgattacatc aagcgcgtga tcgcgacggc
     4981 gggcgacacc gtcgaggttc gctgcaacat cgtctacgtc aacggcatcc ccgtgccctc
     5041 gaaaatgatc gagtccggcg agacctgctc gtacaaggat ttcgacgagc acgacaaccg
     5101 ctggttcacg cggcagtgca gccgctatcg cgagaccgtc aacggcgtcg actacgacac
     5161 ctttcacgat cccgagcggc ctgctcgcga cgcgcggatc gcagaaggca acccgccgcc
     5221 cggcggcgac gcgcgtgact tcccgacgcg tggcaatccg ttcccgccga gctgcagcca
     5281 gtccgacgag gcgctcaagg ccgcgaacat cgtccaggtg aaggggaaga tcgtcgagac
     5341 gaagcccgag gcggtcgcga aggtgtgcga gccccagctg cattacatcg tgccgccggg
     5401 tcacgtcttc gtgcagggcg acaaccgcaa caactcgaac gactcgcgcg tgtggggcgc
     5461 agtcccgatc gagaacatca agggcaaggc gctgttcatc tggctgtcat atgagcactg
     5521 gggcccgctc gactggaccg gcattcgctg ggaccgcatc ggcaacttcg tccatcactg
     5581 acaaatgggg tcagaccccg tttcgggcat ggcgcgtgct gaacgcgtcc gccatgacga
     5641 cgaaacagac acgattcgcg acaacgatgt cgcggtttgc cgtgctcgcg gcgatcgcgg
     5701 cgtgcggagg cagcaccggg cgcacggggc tcgacggccg catcgcgtgc ggagacaagt
     5761 tctgcgagac cggccagctg tgcgcgacga cggaggcggg cgtcgacgcc gcggttccgg
     5821 ggcagccgat cttctattgc gtgacggttg cggacggctg cttcgtcgag gattgcctca
     5881 actccgcgtg ccccgtgtgc atccgcgatc tttgctctcc aggcacggcc tacggcgtcg
     5941 agctcgacgg ccgcaatctc acgtgcgccg gcgtgtgagc ccggtcgcta ctgcttcttt
     6001 gccggcagcg cgagcttcgc gaaacattcc ttgagcgcgt tctcgacggc ggtctcggcg
     6061 gccgagtccc acgtgaactg gcgatccttg tcgcggatct tcatgccgac ttcctgcttc
     6121 acggttgcgc gaccatcgcc agtgaagccc atcgtgcggc ccgggatcgt ctcgccgagc
     6181 acgtgcaggc ccacagtcac cgagatgcgc tgcgcgttct ttttgtcctc gagcgggacc
     6241 acctcgatcg acgcctccgt cacctcgacc gtcacgaggt acgccgcggc gacgcccttc
     6301 ttcgtgagga acttgcggta ggtggtgccg ttgttcgctt cgggatcggc aggagcgcct
     6361 tcgagcttca ccagctgcgg gtgactctcg agcgctttct tgaagaccgc ctcgatccga
     6421 ggctgcgcct ccttggcgac gtcgggcttc acctcgggtt tcacgaggac cttggtgagc
     6481 tcgaagtagt acttcttctt cggcggcgca gcgtctgcgc gcggggcgag caccacgagg
     6541 gcgcagacga caagaaggag gctcttcatc gggcgcatca tgcctcaact gacgctgcgt
     6601 gctacgtttc gattcatggt gcggccgcgt tggcacgacg cgttgatcgt cagtgcgatc
     6661 gtggccctgc tcgccgtcgg ggtgtgggca ctgtggtggg aagacgtacg aaaccttctc
     6721 cacctggggc ctggtgaagc ggcctccccg ccaccggccg cgcctgcgaa tccgcagacc
     6781 taagcgcgtg gtgaacttga ccgtggttcc caacgcttag taccgtttcg gttcgggagg
     6841 ggccccactc gtatgcggtc ccgtttttag aggcccgacc tccaggagcg catgttcacc
     6901 atcatcatcc aagaaaaagg cggcgagcag cgccgcatgg tgttcaacaa gccagaggtc
     6961 accattggcc gcgtacaagg caacgacatc gtgctgccga agggcaacgt gtcgaagcgc
     7021 cacgcgcgca tcgtgctcaa ggacggcaag ttcatcatcg tcgacctgaa gagcacgaac
     7081 ggcacgtacg tcaacggtcg caagatcacg agcccgctcg tcgtcaaaga ctcggacaag
     7141 atctacatcg gtgacttcat cgtcggcgtc gacgaagccg cgaacgcgga aggtgatgga
     7201 ccgtcggaga cgacgacgtc gccgcccggc gccgatcgcg gcgggccgat cgttcccgat
     7261 gcgcggccac cgcggccgac cgaagctgcg ccgcaaccga tgccgattcc gatgggcggc
     7321 ccgatgggcg gcggcatgag tggcggcatg ggcggcggta tgggtggtgg catgggcggg
     7381 atggatggct ccgaccgcat gcttcccgag cgaccggctg cagccgggcc gtcgcaaagt
     7441 gttccgcctc cgatggcgcg tccggccggt ggtccgccgc gtccgccggg tgcgctcggt
     7501 ggtccgccgc gcgaaccgcc tgggccacgc gaaccgcgcg agccactcgg tggtccgcgt
     7561 ggtggagata tggggggacc ggggcgcgag atgccgccgg tagcgccgcc gccgtcgaac
     7621 ctccgtccgg gtgcgcgtcc cggtggcacg atgccaccgc ccgtcgcgcc gctcggctcg
     7681 ccgccggcac ctgtaccgcc gccgccggcg atgggtgatt tcccgccgcc tgttgcggcg
     7741 cctgcgccgt cgccgatgtc ggccgcacct gccatcgcgc cagcaccgct gccgagcgcg
     7801 caacccgcgc ccgtcgttcc gctcaagaac gagcagcgtg cacgcgccgc gcagccgcag
     7861 cccgggccat ctcgcaaggt cagccgctcg gtctcgcccg tcgcgaagcg cggcgtcgca
     7921 ctcgagccgc tcgacagcaa gatcgtcaag atgctcgacc tgcagtcgaa catcctcgag
     7981 cggctgcgcg cgaagctcga tctcgataag gtgccgatcg agcgcctgca cgaggaagag
     8041 ctgtggcaga aggccgagcg cgcgacgatc gatctcgtcg agacgctcga gaccagcggc
     8101 gagctgccga agtacatcga ccaagacgcg ctgatcaaag agacgctcaa cgaggcgctc
     8161 gcactcggcc cgctcgagga tctgttcgcc gacgaaggca tcgacgagat cctgatcgac
     8221 cgtcgcgatc gcgtcgtcgt cggcaagaac ggccagctgc gcggttcggg taaagcattc
     8281 tcgtcggacg aagtgttcga gcgcgtcgtg aagcgcctcg tccacgaagc gggcgccgtc
     8341 atcgacgagt cgcgcccggt cgtcgacatg cgtatgcgcg acggcacgcg gctcaccgcc
     8401 gcggtgtcgc cggttgctgc gcgtggcgcg tgcctcgtgc tcaagaagcc cgcgtcggca
     8461 acaccgaccc tgtcggagct cgtctcggca ggcacgctgt cgagcggcat gagcgacttc
     8521 ctcaccacgt gcatcaacgc gcgccgcaac atcctcgtgt gcggcgcccc gggctcgggc
     8581 aagaccacga tcctcggtgc gctcgccgcg gcatcgccgg ctggcgaacg cgtcgtatcg
     8641 atcgagaacg tcgccgagct cgcgatcacg cgtgacgaat ggatccagct cgagacgcgg
     8701 cccggttcgg gcaaggcaag cgacatcgat ctcggtgcgc tgctcgacac cgcgatgcgg
     8761 ctgatgccgg atcgccttgt cgtcggtgac gtccgcggtc gtgaagcgat gcgcctcatc
     8821 gaggcactga gctcgtcggt cgacggcgcg gtcgtcggcg tgacggggga gggcgcgaac
     8881 agcgtgctca accgcatcgc gacgctcgcg cgcgtgacgc agccgtgggg ctcggatgcc
     8941 gcggtacgcg agctcgtcgc gacggcgttc gagatcgtcg tgcacgtcgg tcgcacggcg
     9001 gatggcgcgc tgaaggtgca ctcgatcgaa gaggtcaccg gcgtgtccga cacgcacttc
     9061 gagaccgagc tcgtcttctg cttcaaggac ggaagcttcg ccgccagcgg caaggtgccg
     9121 agcttctact cggccatggg cgccgaccaa gcggtcttcc gctaacagaa ggcaaccacc
     9181 acgaccgcgg tgacgccgga tcaaacgatc cgcggcgctg tattgttgca tgcgggagca
     9241 ctccgcgagc cgttaccatc aacggatgga gccgcgcgtc cggttcatcg tcgatttgtc
     9301 atcagacctt gcgtcactcg acaccttccc gttgcgctgg cggtggactg agcagcggca
     9361 cacgttgctc tcgccacacg agctcgctcg cgtccgccct ctatcgacac agaaagcccg
     9421 tgacgcttgg gacctttcgc tgtcatggca tgcgcgggaa atggaccagg ccacaccgtc
     9481 cgagaagtta ttcatcgtgg agactctcga ccgcccgcat gaacgggacg gtactggatg
     9541 gcttcgggct cggctgcctt cggagccggt tccggtcatc gtctcttggc agcctgactg
     9601 ggctgtcctg accgacgcgt ccttgttcgt cgagcgctgg gagacattct gctatccagc
     9661 tagcgatgac gtggatatct ggcctctaga tggccgctgg ctggctgtct actggcacga
     9721 ggagcagctg gcctatggcg cgcgtcgggc cgacgagatt cgaacctagc gtctgcggcg
     9781 ccgtggcgct tcaacgtctt cgggctacgt cgactcgacg cggcgcgcga gctcgagcac
     9841 ctcgagatcg cgtggccaca ccgcgcgcaa caggtccagt aggcccttcg cgctcgcctt
     9901 gtcgccggcg tcgatgcgct cgcgcgcacc gtcgagctgc gcccgcttga gatttggcgc
     9961 gagcagcgac gccttcgtcg cggcctcggc acgcaccatc tcgtcaccgg cccggctcgc
    10021 aagccgggcg acctgcgacc agaagtagcc ggcctgcgga tgctgcacac cttcggcagc
    10081 ttcggcggcc atgcgcgctt cgtcgatcgc gccggtgctc tcgccaaggc gctcggacac
    10141 gcgggcaagg tcgagccacg cccacgcgaa caccggatcg tcggcgacga cttgctcgag
    10201 ctcgttgcgc gcgtcctcca tcgcatcttg ctcgatgagg acgtgagcga ggcgccagcg
    10261 cggcgccggt gcggctgtgt cgcgcttgat catcgcgcgc agctgctttt cgcgcacgat
    10321 cggcaggatc tcgccgtcgt cgtcgaatac gtcgtcggca tattcgccgt cgtgatcgaa
    10381 gatcaagtcc agcgcatcga gctggccggc gagccagcga tcgatgcgcg tgccgtcgca
    10441 aacttcatcc tcgatcgatt cgtcgttgcg ccagaccttg cccttctcgt cgacggagat
    10501 cgcgtcaccg agtgcgctcg cgaacaacca gcggccgttc tcgtactgga ccgcgtgcga
    10561 gccgaggatc tcgacgctct cgacgaagat gcgggcgccg tcgcagcgcg cgtacagctc
    10621 gatcagcgca gcgggcaggc cactcggcag ctcctcggcc ggcggatcga cgtcgtgcag
    10681 accgggcggc gcgtcctcga gcttggcaaa aatgcgatcg agcggctcac cgtcgtcgcg
    10741 cttcttggcc atgccgcgat cttactgcga ggtcagcggc agcgcaccga ccgcgacgat
    10801 cggctcgccc tgagcgtcgg cgccccggaa cactgcgacg attcgcgtac cggctcccgg
    10861 cgcggcgagc cggacgagct cgtccttgcc accggcggtc tgcgcggtgc cgatcgacgt
    10921 gcgcacgccg cgatcatcct cgacgaggac atcgagcgaa ccggcgccca ggcccttgcc
    10981 gctgacgacg acctgctggc cgccggtgat cgtgagctcg ccgcggagca ggaagtcgag
    11041 tagcccggtc tcgtacgccg cgacctcggg caggagcaca gctacctgct cgagcatgca
    11101 gtcgtcgtcg aggctgaacg acagcacacc gtgatcgacc ttgtagcggg cgaggcacgt
    11161 gcccgcctga ttgcggagcg tggtgccgtc ttcgcggctc gctgccatca gattcaagcg
    11221 cgacggcagc gccggctgcg gacgcgcgag cttgggcttc gtctcgtcgc cgacgcgcgt
    11281 cgattgcggc agcgtgttcg gcgagaagta ggagcgcgag accatgtcgg cgagcccgcc
    11341 gccatccgtc gcgctgaaga agtcgtgcat gtgatcgcgc gtgatcaccc gcgatggcgc
    11401 cggaacgccg aggcggccgt acgcgagcgc ggcgatccgc tcgaagcgtg aaccgagatc
    11461 gtcaggacca ccgccgagtg gctcgaagtg cgcggcctcg tcaccgcgca cgcgcgacgg
    11521 cgaaccgaga tcgccgagca cgtggatgat cgcgcccgcc gcgatcaacg cggccgccat
    11581 gtggcgcgag cgttcgcccg gcgtcgcggc gctcaccgcc ttggcgtact gatccatgaa
    11641 cgcgccgagg tggaatggat tgtccttcga cgtgatccag tccggcgccg gcatgccgtg
    11701 cgcgggcaag gtgacgcggc cgatcgcttc gcggacggaa tccgagaggc ccttgaacac
    11761 gccgcgctcg gggcgctccc agcctttgtg cgtcgccgga tcgtagaagt gattcaccgc
    11821 gtggcgcgac ggaacatcgg cgagcgccgc gccggccgcg atccacgcga gcgcggtctg
    11881 gcggccgcgc gaatctggca ccgagccatg gctcggcgac agcagcttga gatctttcat
    11941 cagcgccggc gcgtcggcgg gcggaatcgt tagcggctcg aacaagccgc cggtgaaacc
    12001 gagcgagacg aggcgcttgt tcagtcgcga cgacatggca gcttgctcgg cgagcccggc
    12061 ttgcgtcgtc tgcgactccc acgcgtgcgc gctcgatgcg aacaagacga gcgacagagc
    12121 tgctgcgacg cgcttcactt gttacctccg gtcgccggcg tgagctgctt gagtcgattg
    12181 tagacaccgc ttgcgagcgc ggtcgcatcg tcgagcgtcg tgcactggtt cacgccgcac
    12241 gtgagcagca cgacgacgcc gcgggttccg tcgaggaatc cgacgccgcg gatgtcgttc
    12301 tcgttcgcga tgaatgaccg gctcgcgatg tcgtccttct cctgcacgcc gggcagctgc
    12361 gtgagcagct cctcgtagcg ctcctgcgct ttggccggct cgagtcgcca gaggcgaatc
    12421 gcgacgtccc acgtctccgg gcgcgcgagc gctttgaagt gttgcgacga gtacgtcgcg
    12481 gtgtcgggct cgtcggagag cggaccggtc tcgaactgac cctcgtactt cgtccggttc
    12541 ttgatgtcgg tatcgctgag cagctgcgcg gcgacgacgt agttgccctc gcggccgggg
    12601 cgcaggtcct cgctcgacgg ccgcttaccc tcgagctccg cgctcgcgtc ggcgagctcc
    12661 cactgcgtga gtgcagcgac cgcggtgccc gaaccgacaa agcgaaggtg ccgcgcgtgc
    12721 gagagctcgt tgcgccagcg ctcgagcagc acctggttgt cgttctgcgc gaagtccatg
    12781 cgcgcgccga gatcatggaa gcggcgatcg ctgcggtacg cgatatagcc gcgcttgtag
    12841 acgagcagcg tgaagtcggt gacgcgcgtg tgcgctggca acgacccgag tgccgcgatc
    12901 ttgtagttgc cgccggcgtc ggtcgagccg acgacctcgc gcgagcctgc aggctcggtc
    12961 agggcggttc cgcgttccag cgtccacgcg gcgtagacca gcgcgcctgc aatcggggtc
    13021 ttcgtggtcg cgtcgatcac gcggccggtg aagggtccgc gcagcgagcc cggatcggcg
    13081 gcgtcggggc gcgatcggaa cggtgcggtc cgaatcgagg actcgacgca tgccgcctga
    13141 acgagcgcca gtaggaggac gacttttcca agcacgtttg cgagtttatg gaccacggaa
    13201 ccggccggtc ttagcaagac ggtacccccg ttgcaaaccg gcgccgcccc atttcccacc
    13261 gcgttgcttt tcgagaaact cggggcaaga ggggtgtgat gctgcgttgt tctgcttgtg
    13321 gccaccagag cccgccgggg tcgtcgttct gcttgaactg cggcaccgcg ctagcgtccg
    13381 cccccgcgca gactcccggc ggtggggcgg ggttgcccgt gatctgctcg gcctgtcggg
    13441 gtgagaaccc gccggggatg aagttctgcc gaaactgcgg cgccgcactt tccggtacga
    13501 cgccgggagc accggcgttc ggcgcaccga tgatggcgcc gccgggtccg ccacctggac
    13561 cgcccttgcc cgggatgccg cccgccggac cgttcgcgac gccgtcgccg atctcaccga
    13621 gcgcgaaggg catgacgcca ccaggtgctc ctcttggtag cgcgccgctc gcgggtccga
    13681 gtgcgacgcc gctgcgagta tccgcgcagg gcagcacgat cacgtgtccg cgctgtggca
    13741 cgtcgacacc gacgggcttt gcgtattgcc agcagtgcgg cctgcacttg aatgcgatcg
    13801 caccgaccga tccaggtgcc ggtgctgccc gaccgcgtcc gatcacgaac gcgcctccgg
    13861 taccgctcaa cgcaccgatc gatccgcatg cggggacgct cgccgcgcca gacggcgccg
    13921 ccattcgcgc aggcgcgatc tccgcaccag cacggtcgag ctcggccgca gcgcccgcag
    13981 cgccgccggt cgtcgtgctg ccgcaagcgc cgacccgtcc atcggctggt tcgtggggaa
    14041 ccgccgtgct cgtcaatcgc gacggttccg acggtcagcg cttcccgctc gcgtccgatg
    14101 acacgatcgt cgggcgcgct ggcgccgata tcgcgttcga cgacgatcgg ttcctcgcgc
    14161 gtcagcacgc gcgcatcgag cgcacgtccg acggcaatat caaagtccac ccgctcgatg
    14221 cgacgaacgg cgtgttcaag aagaccgacg gctccgtcga gctcgccgat ggccagatca
    14281 ttctcgtcgg ccgcgaagtg ctgcgcttcg agcgcgtcgc acaagaagaa cgcaccgtac
    14341 atccgctcgt tcgccacggc gtcgcgctgt tcggttcgcc gccgcgcgat ccatggggac
    14401 gcatcgtgca gctgattccg tcgggcggct atcgcgacgt ccgccatctc atcggcgaag
    14461 aaatcgtgct cggccgcgaa gaaggcgaca tcgtattccg tgacgacgca ttcatgtcgc
    14521 gtcgccacgc tgcgatcacg tgggacgggc ggcgtgcgct gctcaccgat ctcggcagct
    14581 cgaacggaac gttcgttcga ctcagcggac cgacatcgat caagagcggc gatcacgttc
    14641 gcatgggcga ccagttgctg cggatcgaac gttgaccgat acgccgcgtc tccagatcac
    14701 gatcggagcc gcgagcgacg tcggccaggt gcgcgagcac aacgaggatg cgtacctcgt
    14761 cggcaatctc gacaacgcga cggtgatcga gctgggcgag ccgtgggagg cgagtctgga
    14821 tcgcgggccg gtgctcgtcg tgtgcgacgg catgggcggc gtcgagggcg gcgaggttgc
    14881 gtccgatctc gcggcgaaag tgatctggcg cgagatgaag ggcaccggtg cgacgcgcga
    14941 ccccgaggtc tacgcccgtc tgcttcggag agcgacacgc gtcgcgaacc acgacgtgca
    15001 cgcgatgtcg aaacgcgagc ccggtttgcg cggcatgggc acgacggtgt cggcggccgg
    15061 catcgcaggt gatcggctga tcgtcgcgac cgtcggtgac tcgcgcgcgt acgtgctgcg
    15121 ttcaggcgag atcgtgcaag tcacgcgcga tcaatcgctg tcatcggcgc tgctcgcggc
    15181 gggtcacgat gccgagcaag cggagcgcgc gggctctgcg atcctgcagg cgctcggcgt
    15241 tggcgatgac gtcgagccgt cgctgtcgat cgtcgagctg cgccgcggcg accgggtgct
    15301 gttgtgtagc gacggtctcc acgggctggt cggcgatccc gcattacgcc tgctcatgtc
    15361 agaaccgcac accgtcgctg aagcggtctc gctgctggtc accgccgcgc gcgccgccgg
    15421 cggctccgac aacatcaccg cagttgttgc gcacgtcggt ggcgatcgcc tcgtgccgcc
    15481 gaagggcgac gacgatctgc cgacgttccg cgagttcgat ccgcagaagg aaggcgatcc
    15541 ggcgctgacg tcgacctcct atgtcgcgcg caggctcgcc gcgaagatcg gcctgccgac
    15601 gtcgtcgatt ccaccgtcga tcccggcgac cgggcaacat gcaattttgc gctcgcgcgt
    15661 gggcttgcgc gtacccgcga cggcattacc gatcgatgac atcagcgggc ctgcgcgcca
    15721 gcgcctacgt gcgtctgcgc tcggttggtg gtggtacgtg ctgatcgcgg ccatcgcctg
    15781 cgtgatcgcc tgggtggccg gcggtaggta accggcgcta gtattgcgcc gagcgcagac
    15841 tgggacgcgc tagatgcatg cgtgttgtga tcgaccacat cgcaggaagt cggcgcggtc
    15901 agcgccagga gttcccgaca agctcgcgca ttcgcttcgg gcgccatccg gactgcgagg
    15961 tgtcgttcga tccgcatcgc gacatcgacg cgagctcgcg acacgcagag ctccgcgtga
    16021 tcgaaacacc ctctgcgacc gaatccgtgt cgcaggcgtg ggtgctcgtc gatctcggca
    16081 gctcgaatgg gacttacgta gaaggccatc gcgtcaccga gacgccggtg acacggaacg
    16141 ttccagtcag cattgaattc ggtccgggtg gtccccgcat ccgcctcttg gtcggcgatg
    16201 acgcggccat cgaaggcctg cctccggtgc aggtagaacg tgcaaaacgg cgctgggtcc
    16261 tacccgtcgt agcaggcgcc atcgtgctca ttgcagtcag ctttctgctg atgcagtgct
    16321 agttgaaaaa gtcgggaaca tagatgcgaa cccgcgattt ctctgcgaga atttgcgtgg
    16381 gatgaactgc ccttcgtgta gttcgccgtt agagccgaca gcccggttct gcggtgtatg
    16441 cgggtaccgg ctacaggcgg cacgtccgcc cggagcgagc acgcccgcga ctgcatcgcc
    16501 gcaagcacgg ccggcaagta acccggtcat tccgcagcgg acggcaacgc ctccggcagg
    16561 tcaaccgctc aagcagcaac aagcgccgca gaagaccatc gcgcgtgcgg ctccgcgcgg
    16621 tcgcaaccgc gacgacatct atctcgaggc ggtgctcaac aaccgcttca aagtcgaatc
    16681 gaagatcggc gagggcggtt tcggtgcggt gtatcgcggt gtgcagctcg cgacgggtcg
    16741 caaggtcgcg ctcaagctcc tgcatcccga gatgaccaag gacgagaacc tcgtcgcgcg
    16801 cttccgccgc gaaggcatgg tgctctgcaa cctgcgcgac gcgcacacga tcacgacgta
    16861 cgacttcgat caaacgccgg atggcacgct ctacatcgcg atggagctgc tcgaaggcaa
    16921 gagcctccat caagtcttcc acgagcaagc gccgctcgag tggaagcgca tgttcaagat
    16981 cctgatcgag atgtgcagct cgctggccga agcgcaccag caaggcatcg tgcatcgcga
    17041 tctgaagccg gagaacgtct atctcgagag ccgaccgggc aatcccgagt tcgtgaagat
    17101 cctcgacttc ggcatcgcga aggtgatgcg cggcgattcg atcgatccgc aatcgcctca
    17161 gctcacggcg acgggacaaa cgctcggcac gctcgagtac atgtcgcccg agcagctcat
    17221 gggcaagcag ctcgatggcc gcagcgacgt ctacgcgctc ggcgtcgtcg cgtacgagat
    17281 gatcaccggc cgcttgccgt tccccgacgc gaaggggccc gcgggcctca tcacggcgca
    17341 gctcaagcag acgccgcaac ccccatcgca agcgcacccg aaggcgaacc tgccgccgca
    17401 ggcggatcgc acgattctca agtgcctcga gaaagacaag aacaaccgct acgccgacgt
    17461 cacgcagttc tcgcacgcgc tgcaagaggt gctcgacgcc gcacgcgatc agagcaaccc
    17521 gctcgcgacg ccgctgccga tggccgcaca gtcgtcgtcg tcgcgcgcgt cgccgccgcc
    17581 ggcgggcatg gatctgctgg agacgcgtcg cggcgagatg ccgaacatgg cgccgccgcc
    17641 gatgccaagc gcgggtgcgt cggtgcctgc gatgcagcgc ggtgtcacgc ccggcgtcgg
    17701 cacaccgatg acgccgatga tgaatccgca cgttccggcc acgccctatc cgcaagcgcc
    17761 cgcgaaccac gatcatcagc tgcagtatcc gcaggcgcag ccgcacatgt atcccgcggg
    17821 tgcgcagacc gaggtcaacc cgacccaggg ctcgaagaag tggatctggt ggatcgtcgc
    17881 gctgctcgcg ctcggtgcgg gtgcgggtgc ggtgctcgcg ctcgtgatgc agtagctcac
    17941 cgattgagca gcgcacgcgc acgctcttcg gcgaccggat cgtcggcacc gccgtcgagc
    18001 cactgctgat agagcttctt cgcgcgcgcg tcgtcgccgc gctgctgcgc gatcacggct
    18061 tcgagctcga tcgcttcagg gagatccgcg gcgcgtgcgc gtgcccgtgc gcaggcttcc
    18121 gacgcccgcc ggtgatcgcc ccgtgcgagc gcatcgcgcg cgatgcgcag gtccgcgcgg
    18181 gctgcagtca cgttcgcgac gcgatcgggc tcggcggcgg gatccgctgg aatgtccgcc
    18241 ggtggcaccg cacgatcacc ttcgttgcgc agctcgtggc gcaaatcgaa cgcacgcatg
    18301 cggccgttcg cgttcgggtc tgcgacccat agctgcagcg atgcagggtc gaggatcacg
    18361 acgtggcgcg cgcgggcatc atcgatcacg ccacgatgcc cggcgggcct cggcgaatcg
    18421 tcggtgccgc gttgatcgcg cagtacggtc gccatcgccg tgacatccgc gagcggcgcc
    18481 ttcacgagct tggctgcgcg ctcgacgcgc gcgatcgtag ccagcacgcg tcgcgcgcga
    18541 tcgttctctg gatcggatga cagcgcgttc gtcgtgagca cgtcaccgaa cgcattcgtc
    18601 ttcgggctgc gctcgacgat cgccttgctc ggcgtccgtt cgacgatcac ccatttaccg
    18661 ctgccaccat cgacgatcac gagtagcgcg gcaccgagcg tcggcgattg ctcgaccatc
    18721 ttcacggctt cgtcgagcgt cttcgcttgc tcgaggatcg cgcgcgccag caatgcgacc
    18781 ggtcgcgcac tgcgggtcgg gcgcacgtcg ccggtgcgcg cgggatcgac catcacggcg
    18841 atgccgtgct cgttgatgcc agtgacgacg ccggcctggc ctggccaccc gaccgaggcc
    18901 cacgcgaact tgccggtcgg cttcacgatc gacacgaccg gcacggccga atcgccgccg
    18961 tcatccatgc ccgatagcga gaaggtgcga ccgatccata cgcgtgccgg catctgtgct
    19021 tgctgaccga gcacggtcaa gctgtgcgcg aggctgtggc gctcggcttc gccgctgcgt
    19081 cgcgacggcg cgccgacatc gagcacggcc tggctccgca gcacggcagc gaagccaacg
    19141 cccacgccgc tcgcgcccgc gccgcgcgtg atgcccgcaa tgatccggcg gtccgcatcc
    19201 gagagcccgt cgtcgatgaa ccgccactgc catgcgagcc gcatgttgtg cgtgaggccg
    19261 ccgaaccagc cttcgtccga gaccgtcgcc tcgatcgatg gccgcgccgc acgaatcgca
    19321 ggctggatca gcggcgcgag cagccggccg tgggccgcgc cgatcgcgtg agattcccct
    19381 tgggcgcgca gcaccgcgat cccacccaac cactcgaggc tggcgttacc aaacgtgagg
    19441 atgggcggcg ctcccggtgc gacctgcgtg cttgcgatct cgccgtggac gtcgccgtca
    19501 ggcacgtcgt acgcgaccga gcggcggtag ataaaccacg cgacgacaag gacgactccg
    19561 agcagggcga acgcgatccc cgcggcgcgc caagggcggg gaaacgccga gcggcggccg
    19621 ccgatcacca agtccggcat gatgcgccga ggtagtacct cgtcccgcga agttgtgcca
    19681 agccgtggac gtaccgtgca cagcacgtgc gccgatgtcg ccgtggggct gcaagtgccg
    19741 tcattccccg aacacttgcg cgcgcaggcg cgagcgtccg cacatcacgc ggtgcgcacg
    19801 gccgttcgca gttgcaactg cgacgccgcg cgacggacag ccgagccgag aacatccgcg
    19861 ttgaccgtcc aagatgcgat tgctagcctt cgataacggc tcgtgtgatg tctgcattac
    19921 cgtcgacgag cgtgaccctg gcagcaacgg aaccgttgct gatcgtggtc gttgccttgc
    19981 tggtcctcgt gatcgcaggc ctcatcgcga tggcgttgtt cgtacgccgg cgcagtcgtc
    20041 cacagcctca gccggcgccg gagccaccgc gtccgcaacc gctgcccgat cgtccgccac
    20101 cgcggccacg tccacgtccc gcgatggtgc gcggcggagg cggtggcggt ctcgcggtcg
    20161 cgacgtcgag cgcgatggtg tgcccgacgt gccgcaccga gtatcacggc atggcgtact
    20221 gcacgcgcga cgcgcgccgg ctcgtgccgc ccgaggacat gctcggtggt ccgctccgcg
    20281 cgatgtcgag cggtaagagt gcgggtctcg tgtgcatggc gtgccgccgc gcgtacgaac
    20341 cgggcctgcg cagctgcccg cacgacggca acgagctcgt gccgttcccg gtctacaacg
    20401 cgacgcgacc gcgcagccgc cgcaattcga ccgagccagc tggcgtcgtc gcgcgcatct
    20461 gcccggtgtg cggcacgaag tacgatctca acgctcgctt ctgcggccac gacgcaggcg
    20521 agctcgtcgt gatcaattag aacgtgcgaa ggcgggtcgt gtagctcgtc gccgcatctg
    20581 gctcgatgag gacccggaag gcaccggtga cgcccactag atcgtctcga cgaactcgat
    20641 gcgacgcgga actttgtacg gggccaggtt gcgcgcgcag tgatcgatga tctcttccgc
    20701 gccgcacttc tgcttcgtga cgacgcgcgc gacgaccatt gcgcccgcca tcggatcggt
    20761 gacgaccgtg gcttgcgccg ctttgatctt cggcattgct tcgaggcagc cctcgacttc
    20821 gccgagcgcg acgcgtttgc catcgacctt cacgacatcg tcttcgcgac cggtgatgca
    20881 gagccggcca ttcttgtcga gcttgccgag gtcgccggtg cgcagccagc cgcccttgtc
    20941 gatcgagccg atcgcgacca tgccgctcgc gcgcgtggcg gccggctttt cttcgtcgaa
    21001 cggaccgatc gacttcggcg agagcgtctt gctcttcacc cagatcaggc cttctttgcc
    21061 gccggtggct gccttgccgt cgggcgacgt gatcttgacg tcgacgccct cgatcggctt
    21121 gccgacggtg gtcgggaact tgccggtgtc ttcgagggcg acggtcgctg cttccgtcga
    21181 gtggtagcag ctgagaatgc gcacgccgta ttcattgagg aagctgtcgg cgacggtctg
    21241 atcgaggcgt gagccggcgg cgagatactt cggcttctcg agctcgagtt tcttcacggt
    21301 cggcagcttc gatagctcgg cgtacatcga cggcgtgccg ggcagcacgt cgacttcatg
    21361 ctcgcggacg agcttgccga tgcggcgcgc cgagatttcc tcctcgagga acatcgtgca
    21421 gccgagcttg agcgtcggca gaaagccaag gtcccagccg tacgcgtgga acagcggcac
    21481 cgcgacgagc actttcgtct tgtcgttcat gccgagcgcg gcgatcgcgt gatcgaccgc
    21541 ggcggtcgtg ttcttgtcgg tgcgcaggac gcccttcgga tcgccgagcg aatcggcggt
    21601 gaacagcacc gcgatcggat cgacgccgtg atcgggcggg ttgcgcttgt agaccgagca
    21661 cgtgagcagc gtgccttgca ggcgacggcg cacttcggcg gcgtcggcgg ccggcgcagg
    21721 cggcggttgg ccgggcggcg tggtcggccg ctgtacgcgc gagcggggca gggtgctcgg
    21781 cggcaccggt gcgctcgtgc cgttcgcgct gatcgagcct tcgcttccac gcggacgcgt
    21841 gacgagggcg cggagcggcg cggcggccag gatgagctcg agctcgcggc cgcccgtggt
    21901 cggatcgatc gggacaatcg ccgcgtcgag cttggacagc gcgagtgcga gaatcacgaa
    21961 gtcggggacg ttgccgagca tgagcccgac ccaggcgccc tccttgatgc ccatgctctt
    22021 gaactcctgc gctcgccggt cgacccggtg cagcaggcct cgccacgaca ggtagttgtc
    22081 gcgatagaca accgccttgt tgttgccctg gacctttgcc gcttttttga gccagtcgta
    22141 gagaagagtc atcgagcctc gtactgcggc caccgtcgag ggataggtaa tttacccatc
    22201 ccagttagac gagggccgac cgcactgtac caggcggttt cggggtcctc aacgtggact
    22261 gatggcttga tttgaccgcc attctggggg acgaaggcag ccggaaatga gcgcggcgca
    22321 gaggccccag accgaagaca gccccgaatc ggcagacgcc gagggttggg cgctgctcga
    22381 cggccttcgc cgtcgcctgg acgagcaggc caaccagaac cgcaagaccc agagcgaggt
    22441 ccgccagctc gccgagtcga ttggcgccat ggtcgagcag cagcgcaagc ggtcgcgctg
    22501 gctgaacgtc aactcgttcg tcgcctacgt catgttcacg ctgctgtgtg gcggggcgtt
    22561 ctatttcgtg tacgagagcc gcgcccgcga gctggtcgcc gagcgcgagc gcgtaaagac
    22621 cgagcgcgac accgcggtgc aacgtgcgaa cgcggccgag aagaagaccg cggcccgcga
    22681 ggccgccgat accaaggcgt gggacgcgta tcagctgctc gaagcgggta agcgcgaaga
    22741 cgccgcgaag aagctggtcg agctgcaagg cgcgccgctg tcgaagttcg aacgcaccgt
    22801 gctcgagacg aaggcgaagc aagccgaggt cgtgcagatc gatctcgcac tcaaaggtgc
    22861 agccgccgcg ttcaaggcgg gacgtcacgc agaagtcgtc gcaccgctcg aagctgcgct
    22921 cgcgctcgat ccaaacggac cgcgcgcgcc gctcatgcac tactacctcg gcgtcgctgc
    22981 gtcgaaggcc ggcgcgctcg acgcggcgac cgcgcacctg caagccgctg tcgatggcaa
    23041 cgtcgatctc gaagacgcac ggttccacct cgcgtccgtg ctcgatcgag cagggcagtg
    23101 gggcaaggcg aagatcgaat acgatcgctt cgcgaccgcg cacccgatgt cgtcctacac
    23161 ctggtacgcg acgcgtcgcg gtggcgcgct ctcgcgtatg cccgcggtgc ggccgccgaa
    23221 cgtgacgcag gagatggcgg cgactcagca gtcggcgctc gatcacgcgg gcacgacgct
    23281 gccgccgtcg gcaaaggcgc ccgcaaccgc agagccaccc gcgccgccga cgaaggccgc
    23341 accgaagccg gccgcgccga aggcggtcgc gccgaagccg gccgcgccga accagcctgc
    23401 gccgaagacg ctcgacttca cgaacgacaa tcccgccgcc gacggctcgg cgtcgcagtg
    23461 acagcgctgc tgacgccgcc ggcgcagcag tactccgcgt cgattcacaa gctctcggcc
    23521 gacgtgtgcg cgcggatcac gaacaagtcg tggcgcgacg atccgcgctg cccgtcggtc
    23581 gccgacctcg cgtacctcga gatcgcgcac gtcacgttcg acggcggtgt cgcgcgcggc
    23641 gagctcgtcg ttgccgcggc actggccaag cgtgccgtcg agctgtttcg ccggctgtac
    23701 cagctcgggt ttccgatccg ccagatgcag ctcgtcgacg actacgatgc gtccgacgat
    23761 gcgtcgatgg ccgccgacaa ttcatccgcg ttcaacttcc gcgtgatcgc cgggacgaag
    23821 ctgctctcgc agcacgcgct cggccgcgcg atcgacatca acccggtcga gaacccgtgg
    23881 cgtaaacccg atcgcatcgt gccgcccgct ggcgagcgct ttgccgatcg caagaacgtg
    23941 cggcctggca tgatcgtgcg gccgggaccg atcgtcgccg tgctcgacga gctcggctgg
    24001 gaatggggcg gcgactggcg tcacgccttt gacgaccatc acatcgtatg gtcgcgacca
    24061 ctatgaagac gctcgccatg ctcctggtgc tcgtctgcgc gtgcaaagac tcgccgtgcg
    24121 acaagtacgc cgatcgcctg ctcgcgtgta atcccaagct ccccggcgat gttcgcgaga
    24181 acgtctatgg ctactgcgtc gccgcgacga tccatgagcc cgagccgaat gcagagccga
    24241 accgaggcga cgtgctgatg tatcgcgcgg tcaaggcatg ctcgccgacg cttgcgtgca
    24301 cggacttcct cgcgtgcctg caggagcaga actgcacgct gatcacgtcg atcaacacgc
    24361 ccggcgagct cacgtttcag tgctggccga cgccgccgcc gcccgccaag ctgcctcagt
    24421 gaatcgcgcc gacgcggctc cacttgcgat cgccgcccgg cggatcgttg atccacactc
    24481 caagcacgcg tccgcgcacg gcgtcgactg acacgacgcc ccagtagcgc gagtcgttcg
    24541 cgttgttgcg gttgtcgccc atcacgaaca gcccgccgtc gggcacgacg aagtgcgcct
    24601 gctgctcgca cggcttggcg tcgtggacgt tgacgacgat cttgccggtg agctgaccgg
    24661 cactgggcgt gggccgattg tagaactcgc cctgcgtgca gctcggcgcg atcagcttct
    24721 ccatgttggg gaagtcgcgc gcgtcgccgg cgggcagcgt gtcacggtgc cgatcgcgct
    24781 cgggccggtc tgggtcgtga aacacctcgt acgtttggct gccgatgcgc tcgcgatacc
    24841 ggctcgtcgc gcgcgtgtac caccgatgct cctcggggtc gtagtcgtcg tactggtcac
    24901 tcgcgaccac gagctcgttc ggaatcgcga cgccgtcgac atagacgacg ttgcagcgca
    24961 cttcgaccgt gtcgccgccg accgcgatca ctcgcttcac gtaatcgatg tcgtgcgtgc
    25021 agggatactc gaacacgatc acctcgccac gctccggcgc acgccagtac agcgacagct
    25081 tgtcgacgaa gatgtggtcg ccgatctcga tcgtcgggta catcgac
//
LOCUS       DASUFX010000511         8068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_221310,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000511 DASUFX010000000
VERSION     DASUFX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221310"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VFV99_20515"
     CDS             <1..1165
                     /locus_tag="VFV99_20515"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5061768.1"
                     /translation="KLSSPSDLIDDLDALLDARGHNLRADRLRRDGLDIATAHAVDRA
                     AKQLDRIVDTRSKTPNGDDAVDRELMIAILTAYPDRVGRRRTAKSADIVFAGGGSGTL
                     APSSAVIEPELVIAVDVSETGGRGQASRVQIRRASAVEASWLLDLYMDHIAETDELVW
                     NAAKERVERVVQMTYDGLPIDEQRDVDGARKAGGRAAELLAKQAIAAGIERFVDGDAL
                     AQWRARVSFVAKIAPESGIVAPNDDALASVIARACEGAISFDELRKLGLLDLLDAQLA
                     DKRALVQRLAPTHLTLPHRGRRAPIHYELDKPPWIASRMQDFFGLARAPSVGDGRVPL
                     VLHLLAPNQRPVQVTTDLPGFWVKHYPALRKQLMRRYPKHAWPEDPTRLIDDD"
     gene            complement(1200..1835)
                     /locus_tag="VFV99_20520"
     CDS             complement(1200..1835)
                     /locus_tag="VFV99_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061769.1"
                     /translation="MSYRNDLDALAARHDALATEVATKTKELDAARHLLDEAQQRAKL
                     PVLPNIRVATPCTADWNKMTGDDRMRACADCNKNVYNISELTRDEAEALILAKEGRLC
                     VRYYQRKDGTILLKDCVIGIAQSRKRRVIAAGAAALLAGGGFAAYRLTRQEPAPRMTM
                     GEVTAETTSMIPDRNTQHVNAPPHARRPHPESIDPSEVKGKLEVMMGDYIE"
     gene            complement(1881..3860)
                     /locus_tag="VFV99_20525"
     CDS             complement(1881..3860)
                     /locus_tag="VFV99_20525"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061770.1"
                     /translation="MDDAWDETQSIGTLRSDDLARAGEVFRYNRYLYRTSGAHLSNGG
                     MGTVYELERRDDESGIVENVVGKVFHSNYLYQLRTDEVTRRDHHHNLAAMARIAAIEH
                     PNILPTYVSAPISDNYLFVTPRMGMTLLEAISKHNLTPRARTKLLVQALEGLSTLHAA
                     RLIHRDFTLRNILVDQGANVAYLFDFDLAMSLDDIGNQTFRNYYKGRIFGSPGWSVAP
                     ETVDQALMDSNITTSLDIYAIGGALHGLFTEQLLYGPADDMWALLIRIAEGVVVGGRS
                     KVHYPDNFPTVLRAVIEGCLEREPQQRFPSVHAVISELRGLLRELPDETVRKSRTRLQ
                     TSAEEDKARAVSVVMASTPDPTITEDVIKQAELAIWEWGYSVERALGRVRGHPIFYAT
                     PRQDLIQGGQFPDANTFPKLVTVIDLTKVADPRGFVENWQRYFWPTLKRVRQGMLTSL
                     HKVIYDANSSSLLLFSEYVDEPRFGNAINETDMHIDAGLALGFLVIRQVAALHEHGMA
                     HNNISPGALLFKGVTATRTVVPAMIGLVEPAMGAEAMAGDCRQLSAMILQWLRPARVA
                     ALNVRIKPMFEALRTKLSTWAFDKGQPAPTIDQLMALMSDALALVDFNFSVLRDSGGD
                     LQEYALLLISLRLYHVLWAELVTEPVRPSLPQVKR"
     gene            3983..4906
                     /locus_tag="VFV99_20530"
     CDS             3983..4906
                     /locus_tag="VFV99_20530"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5061771.1"
                     /translation="MLEHRFAKLAAGATFLLLVIGGTVNPTGSSLACPEPTLVCHGQL
                     FPPMVGGVFYEHGHRLAAMSVGLLQIALTFLLLRRTPMKRLAWLLLGMVIAQGALGAI
                     TVKYKLPWFVSTAHLLLGMSYFATLIYTAFRTRPAPSALELDRHEKLRSELGSARTWI
                     RVACGAVFVQLLLGALVRHLGAALVCLGVPTCTMGGDWWPDSNVQHLHMIHRGFGVVV
                     GIVTILAAIQVYRRTKSWPQLRVLSMIAPLLVVAQIALGIYTVLTFRSVPVAVGHFAG
                     AVSLWALWMSALLMTRRRQPPVDGATLVEVP"
     gene            4903..5763
                     /gene="cyoE"
                     /locus_tag="VFV99_20535"
     CDS             4903..5763
                     /gene="cyoE"
                     /locus_tag="VFV99_20535"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5061772.1"
                     /translation="MTARALTAKTFDLVALVKPNIMIMALLTAAGAMSLAPGTVATAQ
                     ALWLLAGTALIVGSANTLNMWLERDIDCLMARTKNRPLPGHRLEPITALAFGALQGVL
                     ALPVLAVVGFIPAALGLLALILYVGAYTPMKQRSHWATWVGAIPGAMPALMGWTAATG
                     HIDAAGLAVFGVLFFWQIPHTHAITIYRQREYEAAGLKTLPNTYGVASARRAILGFLI
                     VQVAVSIMPALLGVAGLPYLAAAVALGVLVLAQAFLGDGSSKWARNVFLTSIVYLPIL
                     FAVMVASGQA"
     gene            5765..6208
                     /locus_tag="VFV99_20540"
     CDS             5765..6208
                     /locus_tag="VFV99_20540"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX5061773.1"
                     /translation="MQVEQIHPAINAALNLTSFVFLVAGRVAIARGDEATHKRRMLTA
                     FTASCVFLVSYLIRFATTGAHKYPGEGWDKTIYLLVLFSHMLFAVVLVPLLLGALRRA
                     LRGDFVAHKRLVKFTFPIWFYVSITGVLVYLMLYHLAPVLHPVTT"
     gene            complement(6198..>8068)
                     /locus_tag="VFV99_20545"
     CDS             complement(6198..>8068)
                     /locus_tag="VFV99_20545"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5061774.1"
                     /translation="SMGGAAGEALEPSDPDGAFEIPGMRPGSYMMAAGAEGSMIEIGK
                     SVQVVDKDVTDVIVELSSGVTLSGRVEPAAVAELGLEMEGEVGIANMFEMVKSAMCRG
                     TSDASGAFTLKNAPSGSFKLVARTKEGRTGKLPITIAKTDLGGLVVTITPRASIAGRV
                     VDTNGKPVAGIGVNAHPDEDKPRSFSMSMGDMREGATSAADGSFKIVGLDAGKYEVRA
                     RNRDDFMAMMRKDKDKKKVIVELAEGAEKTGVVVTVEARDGVIRGVVMGADHKPAADS
                     WVTARMEPDKDDEIAKKMPERFMWLGQSEPVLTNGEGAFTIGRLKKGKYTLIAEGPKG
                     NSHGEKAGVATGDSATIQLARLGTLTLTVKQAGKPVPAYDASCDGPGDDVQRHVAAED
                     GSYQFDHLAPGDYTCNVRSDTGTAEGKVTVPADDVKLDLTLTPWGSLTGVVVSVLDGK
                     PIPGLTVAAMGGANDPTAMFDVLKGGGAKTDPTGRFVVEHVATGKGRVLFIPKDGAFQ
                     NMDSHDYEAKAGQRTDLGTIKIVPPRNGDAGTFGLSTEVDGETLKVTSVKEGGPAAGA
                     GVQEGDKITAIAGQPLDKLGVEIGHKLLESGGPSPGQTVSITLQRGPTVTLTAIKW"
BASE COUNT         1213 a         2771 c         2561 g         1523 t
ORIGIN      
        1 gaagctgtcg tcgccgtcgg atctgatcga cgacctcgat gcactcctcg atgcgcgcgg
       61 tcacaacctg cgcgccgatc gactgcgccg cgacggcctc gatatcgcga ctgcccacgc
      121 cgtcgatcgc gctgcaaagc aactcgaccg catcgtcgac acgcgctcga agacgccaaa
      181 cggcgacgac gcggtcgatc gcgagctgat gatcgccatc cttaccgcgt atcccgatcg
      241 cgtcggacgc cggcgcacag cgaagagtgc cgacatcgta ttcgccggag gcggcagcgg
      301 cacgctcgcg ccgagctcgg ccgtgatcga gcccgagctc gtgatcgcag tcgatgtcag
      361 cgagaccggc ggccgcggcc aggcgagccg cgtgcagatc cggcgtgcga gcgcggtcga
      421 ggcatcgtgg ctgctcgatc tctacatgga ccacatcgcc gagaccgacg agctggtgtg
      481 gaacgcggcc aaggagcgcg tcgagcgcgt cgtgcagatg acgtacgacg ggctcccgat
      541 cgacgagcag cgcgatgtcg atggcgcacg caaagccggt gggcgtgccg ccgagctgct
      601 cgcgaagcag gcgatcgccg ccggcatcga acgcttcgtc gatggcgatg ccctcgcgca
      661 gtggcgtgcg cgcgtcagct ttgttgcgaa gatcgcgccg gagtctggca tcgtcgcgcc
      721 gaacgatgac gcgctcgcgt cggtgatcgc gcgcgcctgc gaaggtgcga tctcgttcga
      781 cgagctacgc aagctcggcc tcctcgatct gctcgatgca caactcgccg acaaacgcgc
      841 gctcgtgcag cggctggcgc cgacgcacct caccctgccc caccgcggcc gccgggcacc
      901 gatccactac gagctcgaca agccgccgtg gatcgcatcg cgcatgcagg atttcttcgg
      961 cctcgcgcgc gcgccgagcg tcggcgatgg tcgcgtaccg ctcgtgctgc acttgctcgc
     1021 gccgaaccag cgccccgtgc aggtgacgac cgatttgcct ggcttctggg tcaagcacta
     1081 ccccgccctc cgcaagcagc tcatgcgccg gtatccgaag cacgcgtggc ccgaagatcc
     1141 gacacggctc atcgacgacg actaggtata tgatcgagcc gtgcgctggg tggtgtgagt
     1201 tactcgatgt agtcgcccat catgacctcg agcttgccct tcacctcgct tggatcgatg
     1261 gactcggggt gcggccgccg cgcgtgcggc ggcgcgttca cgtgttgcgt gttgcggtcc
     1321 ggaatcatgc tcgtcgtctc ggccgtgacc tcgcccatcg tcatccgcgg cgccggctct
     1381 tgtcgcgtca gccgatacgc cgcgaagccg ccacctgcga gcaacgctgc cgcaccggcc
     1441 gcgatcacgc gccgcttgcg gctctgcgcg atgccgatca cgcaatcctt gaggaggatc
     1501 gtgccgtctt tgcgctggta gtagcgcacg cacagccggc cttcctttgc gaggatcagc
     1561 gcctcggctt cgtcacgcgt cagctcggag atgttgtaga cgttcttgtt gcagtcggcg
     1621 cacgcgcgca tgcggtcgtc gccggtcatc ttgttccagt cggcggtgca tggcgtcgcg
     1681 acccggatgt tcggcagcac cgggagcttc gcgcgctgct gagcttcgtc gagcaggtgg
     1741 cgcgccgcgt cgagctcctt ggtcttggtc gcgacttcgg tggctagcgc atcgtgacgg
     1801 gcggccagtg catcgagatc gttgcgatag ctcatagcta cgtcccaacg cacgagccaa
     1861 cgtatcgatc tatcgagtgg ctagcgtttc acttgcggca gcgacgggcg caccggctcg
     1921 gtgaccagct ctgcccacag cacgtgatag aggcgcaggc tgatcaataa cagcgcgtac
     1981 tcctggaggt cgccgccgga gtcgcgcagc accgagaagt tgaagtcgac gagcgcaagc
     2041 gcatccgaca tcagcgccat cagctgatcg atcgtcggcg caggctggcc cttgtcgaac
     2101 gcccacgtgc tgagcttcgt gcgcagcgct tcgaacatcg gcttgatccg cacgttcaac
     2161 gcagcaacgc gagccggccg caaccactgc aggatcatcg cggatagctg gcggcaatcg
     2221 ccggccatcg cctcggcgcc catcgcgggt tcgacgaggc cgatcatcgc gggcacgacg
     2281 gtgcgtgtcg cagtcacgcc cttgaacaac agcgcgcctg gcgagatgtt gttgtgcgcc
     2341 atgccgtgct cgtgcagcgc ggcgacctgg cggatcacca ggaagccgag cgcgagcccg
     2401 gcgtcgatgt gcatgtcggt ctcgttgatc gcgttgccga agcgcggctc gtcgacgtac
     2461 tcggagaaca gcagcagcga gctagaattc gcgtcgtaga tgaccttgtg cagcgacgtc
     2521 agcatgcctt gccgcacgcg cttcaacgtc ggccagaagt agcgctgcca gttctcgacg
     2581 aagccgcgcg ggtcggcgac cttcgtcaga tcgatcaccg tgacgagctt cggaaacgtg
     2641 ttcgcatcgg ggaactggcc gccctggatc agatcctggc gcggcgtcgc gtagaagatc
     2701 ggatggccgc gcacgcgacc gagcgcgcgc tcgaccgagt agccccactc ccagatcgcg
     2761 agctcggcct gcttgatcac gtcctcggtg atcgtcgggt ccggcgtgct cgccatcacc
     2821 accgacaccg cgcgtgcttt gtcttcctcc gccgaggtct gcagccgcgt gcgggacttg
     2881 cgaacggtct cgtcgggtag ctcgcgcaag agcccgcgca gctcgctgat caccgcatgc
     2941 accgacggaa agcgctgctg cggctcgcgc tcgagacagc cttcgatcac cgcgcgcaac
     3001 acggtcggga agttgtcggg gtagtgcact ttgctgcgac caccgacgac gacgccctcg
     3061 gcgatccgga tcagcagcgc ccacatgtcg tcggccggtc cgtacaagag ctgctcggta
     3121 aacaggccgt gcagtgcgcc gccgatcgcg tagatgtcga gcgacgtcgt gatgttcgag
     3181 tccatcaacg cctggtcgac cgtctccggc gcgaccgacc accccggtga gccgaagatg
     3241 cggcccttgt agtaattgcg aaacgtctga ttgccgatgt cgtcgagtga catcgcgaga
     3301 tcgaagtcga acaggtaggc gacgttcgcg ccttgatcga cgaggatgtt gcgcagcgtg
     3361 aagtcgcgat ggatgaggcg cgccgcgtgc agcgtcgaca gaccctcgag cgcctgcacg
     3421 aggagcttcg tgcgcgcgcg cggcgtcagg ttgtgcttgc tgatcgcctc gagcagcgtc
     3481 atgcccatcc gcggcgtgac gaacagatag ttgtcggaga tcggtgccga cacgtacgtc
     3541 ggcaagatgt tcgggtgctc gatcgcggcg atgcgcgcca tcgcagcgag gttgtggtgg
     3601 tgatcgcggc gcgttacctc gtcggtgcgc agctgataga ggtagttcga gtgaaagacc
     3661 ttgccgacga cgttctcgac aatcccggat tcgtcgtccc ggcgctcgag ctcgtagacg
     3721 gtgcccatgc cgccgttcga caggtgcgcg ccgctggtcc ggtagaggta gcggttgtag
     3781 cgaaacacct cgccggcgcg agccaggtcg tcggatcgga gggtgccgat cgactgggtt
     3841 tcgtcccacg catcgtccat ccgtgaggct gcatcataac gtgctgaatt ctctggggtc
     3901 cagctaatgg gcgacgtgtt gtcgcatcac gctcggcggc acgatgcaac ggcatcagat
     3961 gtggtaccca ccctcgttgt ccatgctcga gcatcgattc gccaaactgg cggcgggcgc
     4021 gacgttcttg ctgctcgtga tcggaggcac cgtgaatccc acgggttcca gcctcgcgtg
     4081 tccggagccc acgctggtct gtcacggcca gctgttcccg ccgatggtgg gaggcgtgtt
     4141 ctacgagcac ggtcaccgac tggccgcgat gtcggtcggc ttgctccaga ttgctctgac
     4201 gttcctgctg ctccgccgca cgccgatgaa gcgcctcgcc tggctcctgc tcggcatggt
     4261 gatcgcgcaa ggcgcgctcg gggcgatcac cgtcaagtac aagctcccgt ggttcgtgtc
     4321 gaccgcgcac ctcttgctcg gcatgagcta cttcgcgacg ctgatctaca ccgcgtttcg
     4381 cacgcgcccg gcgccgagcg cgctcgagct cgatcgccac gagaagctgc gcagcgagct
     4441 cgggagtgcc cgtacgtgga ttcgcgtcgc atgcggcgcc gtgttcgtgc agctcttgct
     4501 cggcgcactc gttcgccatc tcggcgctgc gctcgtgtgc ctcggcgttc cgacatgcac
     4561 gatgggcggt gattggtggc cggactcgaa cgtccagcac ctgcacatga tccaccgcgg
     4621 cttcggtgtc gtcgtcggca tcgtcacgat tctggcagcc atccaggtct atcgccgcac
     4681 caagtcctgg ccgcagctgc gcgtgctgtc gatgatcgcg ccgctgctcg tcgtcgcgca
     4741 gatcgcactc ggcatctaca ccgtgctcac gttccgttcc gtgcccgtcg ccgtcggtca
     4801 cttcgccggt gcagttagcc tgtgggcgct gtggatgtcg gcgctgctga tgacgcgtcg
     4861 ccggcagccg cctgttgatg gcgcaacgct cgtggaggtc ccgtgaccgc acgcgcgctc
     4921 accgcgaaga cgttcgacct cgtcgcgctg gtcaagccga acatcatgat catggcgctg
     4981 ctcaccgccg ctggcgcgat gtcactcgcg cccggtacgg tcgcaaccgc tcaggcattg
     5041 tggttgctcg ccggcaccgc gctgatcgtc ggctcggcga acacgctcaa catgtggctc
     5101 gagcgcgaca tcgactgctt gatggctcgc accaagaacc gcccgctgcc gggccatcgt
     5161 ctcgagccga tcaccgcgct cgcgttcggt gcgctgcaag gcgtgctcgc gctgccggtt
     5221 ctcgccgtcg tcggcttcat tcccgctgcg ctcggcctgc tcgcactcat cctctacgtc
     5281 ggcgcgtaca cgccgatgaa gcagcgctcg cactgggcga cgtgggtcgg tgcgattccc
     5341 ggcgcgatgc cggcactgat gggctggacc gcagccaccg gtcacatcga tgccgccggg
     5401 ctcgccgtgt tcggcgtcct gttcttctgg cagatcccgc acacgcacgc gatcacgatc
     5461 taccgccagc gcgagtacga ggccgccggc ctcaagacgc tgcctaacac ctacggcgtc
     5521 gcgagcgcgc gccgcgcgat cctcgggttt ctcatcgttc aggtcgcggt cagcatcatg
     5581 ccagcgctgc tcggcgtcgc cggattgccg tacctcgccg ctgcagtcgc attgggcgtg
     5641 ctcgtgctcg cgcaagcctt cctcggcgat ggctcctcga agtgggcgcg caacgtgttc
     5701 ctgacgtcga tcgtctacct gccgatcctg ttcgcggtca tggtcgcgag cggtcaggcc
     5761 tgacgtgcag gtcgagcaga ttcatccggc gatcaacgcc gcgttgaatt tgacgagctt
     5821 cgtgttcctc gttgccggcc gcgtcgcgat cgcgcgtggt gacgaagcga cgcacaagcg
     5881 gcgcatgctg accgcgttca ccgcgtcgtg cgtgttcctc gtctcgtatc tgatccggtt
     5941 cgcgacgacc ggcgcgcaca agtatccggg cgagggctgg gacaagacga tctacttgct
     6001 cgtcttgttc tcgcacatgc tgttcgcggt cgtcctcgtg ccgctgctcc tcggagcgct
     6061 acgccgagcg ctacgcggtg acttcgtcgc gcacaagcgg ctcgtcaagt tcacgttccc
     6121 gatctggttc tacgtctcga tcaccggcgt gctcgtgtac ttgatgctgt atcacctcgc
     6181 gccggttctt caccccgtca ccacttgatc gcggtgagcg tgaccgtcgg accgcgctgc
     6241 agcgtgatcg agacggtctg gccgggactc gggccgcccg actcgagcaa cttgtggccg
     6301 atctcgacgc cgagcttgtc gagtggctga cctgcgatcg cggtgatctt gtcgccctct
     6361 tgcacacccg cgcccgccgc tgggccgcct tccttcacac tcgtcacctt taacgtttcg
     6421 ccatcgactt cagtgctgag cccgaacgtg cccgcgtcac cgttgcgcgg cggcacgatc
     6481 ttgatcgtcc cgaggtcggt gcgctgtccc gccttcgctt cgtagtcgtg cgagtccatg
     6541 ttctggaacg cgccatcctt cggaatgaac agcacgcggc ctttgccggt ggcgacgtgc
     6601 tcgacgacaa agcgcccggt cggatccgtt ttcgcgccac cgcctttgag cacgtcgaac
     6661 atcgcggtcg gatcgttcgc accacccatc gcggcaaccg tgagccccgg gatcggcttg
     6721 ccgtcgagca cgctgacaac aacgcccgtc aacgaccccc acggcgtcag tgtcagatcg
     6781 agcttcacgt cgtcggcagg cacggtcacc ttgccttcgg cagtgccggt atccgagcgc
     6841 acgttgcacg tgtagtcgcc gggcgcgagg tgatcgaact gatacgagcc atcttccgca
     6901 gcgacatggc gctgcacgtc gtcacctggg ccatcgcagc tcgcgtcgta cgctggcacc
     6961 ggcttacccg cttgcttgac cgtcaacgtc agcgtgccga ggcgtgccag ctggatcgtc
     7021 gccgagtcac cggtggcgac gccggccttc tcgccgtgcg agttgccctt cggaccttcg
     7081 gcgatcagcg tgtacttgcc cttcttcagc ctgccaatcg tgaacgcgcc ctcgccgttc
     7141 gtcagcacgg gctcgctctg gccgagccac atgaagcgct ctggcatctt cttcgcgatc
     7201 tcgtcgtcct tgtcgggctc catgcgtgcg gtcacccacg agtccgccgc cggcttgtga
     7261 tcggcgccca tcacgacgcc gcgaatcact ccgtcgcgag cctcgaccgt cacgacgacg
     7321 ccggtcttct cggcgccctc tgcgagctcg acgatgacct tcttcttgtc cttgtccttg
     7381 cgcatcatcg ccatgaagtc atcgcgattg cgcgcgcgca cctcgtactt gccggcgtcg
     7441 agaccgacga tcttgaacga gccatcggcg gcgctcgtcg cgccttcgcg catgtctccc
     7501 atcgacatcg agaacgagcg cggcttgtcc tcgtcgggat gcgcattgac gccgatgccg
     7561 gcgaccggct tgccattcgt atcgaccacg cggccggcga tcgacgcgcg cggcgtgatc
     7621 gtgacgacga gaccgccgag gtcggtcttc gcgatcgtga tcggcagctt gccggtgcgg
     7681 ccctccttcg tacgcgcgac gagcttgaag ctgcccgacg gcgcgttttt cagcgtgaac
     7741 gcaccgctcg catcgctcgt gccgcgacac atcgccgact tcaccatctc gaacatgttc
     7801 gcgatcccga cctcgccctc catctcgagc cctagctcgg cgaccgctgc cggctcgacg
     7861 cgtccactca gcgtgacacc gctgctgagc tcgacgatca cgtccgtcac gtccttgtcg
     7921 acgacctgaa cgctcttgcc gatctcgatc atcgagcctt ccgcgccggc cgccatcatg
     7981 tagctgcccg gccgcatgcc tggaatctcg aacgcgccat ccgggtcgct cggctcgagc
     8041 gcctcgccgg ccgcgccgcc catcgaga
//
LOCUS       DASUFX010000512         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_776631,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000512 DASUFX010000000
VERSION     DASUFX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776631"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..790
                     /locus_tag="VFV99_20550"
     CDS             272..790
                     /locus_tag="VFV99_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061775.1"
                     /translation="MVVHKTTTMLDVKLDATTLTCADGELRVAAPKLAALTLMSPDDV
                     SLDAPGMSAGTCAPGRMPSDLIDMAHPVESVAVTVKAIRQDHVDLMAMSCYTYLVERV
                     ETTIRGVTFSHEDSTWIGSRPVSECNGGDSPADDPGHVEDDAGNSCSTTRGGSMWIGL
                     ALALGLVLRRRR"
     gene            complement(787..1281)
                     /locus_tag="VFV99_20555"
     CDS             complement(787..1281)
                     /locus_tag="VFV99_20555"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5061776.1"
                     /translation="MDLFVIRHAIAADAEPGQDDPSRELTDEGVRKFKQVVKGVRELG
                     WAFDRVLTSPWERAVATAALLDPVTDGPTIETDLLTEKPRAELLALIAEASSPEDRRA
                     TAVVGHEPWLSELVAWLAFSDTRHSEAIELKKGGIIWLDGTAVPGGMRMRAMLTPKIL
                     RALR"
     gene            1353..1829
                     /locus_tag="VFV99_20560"
     CDS             1353..1829
                     /locus_tag="VFV99_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061777.1"
                     /translation="MSLAIVIAAAACGGKKPAPATTTTEQHAEGEGHGMEHEGMPAEM
                     TKFHDVLAPRWHAEKGPQRMKDTCAAIPEFKADADALAKATPPEKANADTWTASTKAL
                     VDSVNGLDPVCQANDAAKFEEAFHKVHESFHATMAAAGMPMEKHEGGEGGEHEHKM"
     gene            1893..2537
                     /gene="pdxH"
                     /locus_tag="VFV99_20565"
     CDS             1893..2537
                     /gene="pdxH"
                     /locus_tag="VFV99_20565"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX5061778.1"
                     /translation="MAKDLLWKVGEQYEGSPLDPADCDPDPFVEVRRWLKLAVDGGLP
                     TANAMTLATVDERDRPAARLVLLKEVDDRGFVFYTSYDSRKARDLAAHPHAALVIYWE
                     PLHRQIRIEGRVEKISAGESDAYFSTRPRGSQLGAVASPQSEVLPNREALERKVAALS
                     AKYGDGPVPRPTSWGGYRVVPDMIELWQGQPSRLHDRVRYRLLDGSWVKDRLAP"
     gene            complement(2538..>3062)
                     /locus_tag="VFV99_20570"
     CDS             complement(2538..>3062)
                     /locus_tag="VFV99_20570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5061779.1"
                     /translation="ELVPRDGTLLLLQADPIVLPVGIVAMTTAPGVQMVADKLTATRT
                     TDAHIERLTADDFPAMHALATLTKPGPFAARTPLLGEFWGVKERGVLVAMAGERMKHA
                     GFTEVSGVCTHPDVRGRGLARELSALVATRIIERGETPYLHAYASNAPAIQLYESLGF
                     KLRCAMHVAVIARA"
BASE COUNT          509 a         1018 c         1046 g          489 t
ORIGIN      
        1 ggcatcacga cgggtgcgtt gtcgaagctg ttgctccacg acgagcaagt cgggcgcgcg
       61 ctcaacgatt tacgtcgcgc gaagggcatg cggccactcc gctagccacc cgtgaggctg
      121 gttctgcaag cagcgtttag cgggcgttta gcggaggtgc ggtgccgcaa gtgttacgtg
      181 cgcgcgcatg tcctcgctta gccgccttag ccttgttgct ggtgtcctct gcatttcgtc
      241 gatcgcggtc gccgcgccga ccgttcgcga catggtcgtt cacaaaacga cgacgatgct
      301 cgacgtcaag ctcgatgcca cgacgctgac gtgcgccgac ggcgagctca gggtcgccgc
      361 gccgaagctc gccgcgctca cgctgatgag cccggatgac gtcagcctcg acgcgccggg
      421 aatgtctgcg ggcacgtgtg cgcctggccg catgccatcg gatctgatcg acatggctca
      481 cccggtggaa tccgtggcgg tgaccgtgaa ggcgatccgc caggaccacg tcgatctgat
      541 ggcgatgagc tgctacacgt acctggtcga gcgcgtcgag accacgatcc gtggcgtgac
      601 gttcagccac gaggactcga cgtggatcgg ctcgcgcccg gtcagcgagt gcaacggtgg
      661 cgactcgccg gccgatgatc cgggtcacgt cgaagacgac gccggcaact cgtgctcgac
      721 gacgcgcggc gggtcgatgt ggatcggcct cgcgctcgcg ctcggtctcg tgctgcgccg
      781 ccgtcgctaa cgcagcgcgc gcaggatctt cggcgtcagc atcgctcgca tcctcatgcc
      841 gccgggcacc gcggtgccat cgagccagat gatgccgccc ttcttgagct cgatcgcttc
      901 gctgtggcgc gtgtcggaaa acgcgagcca cgcgacgagc tccgagagcc atggctcgtg
      961 accgacgaca gccgtcgcgc ggcggtcttc cgggctgctc gcttcggcga tcaacgcgag
     1021 tagctcggcg cgcggcttct cggtcaacag gtcggtctcg atcgtcgggc cgtcggtgac
     1081 cgggtcgagc agcgcggccg tcgcgaccgc gcgctcccac gggctcgtga gcacgcggtc
     1141 gaacgcccag ccgagctcgc gcacgccctt aacaacctgt ttgaacttgc gtacgccctc
     1201 gtcggtcagc tcgcgtgacg ggtcgtcctg cccgggttcc gcatcggccg caatcgcgtg
     1261 gcgaatgaca aacaagtcca tgcgcgcgac tctacccggc tgcgttgaca cggcgagtgc
     1321 ttgcatgcga aagcgacgcc atgattcgca ctttgagcct cgcaatcgtg attgctgccg
     1381 ccgcgtgtgg tggcaagaag ccagctcccg ccacgacgac caccgagcag catgccgaag
     1441 gtgaaggtca cggcatggag catgaaggca tgcccgccga gatgacgaag ttccacgacg
     1501 tgcttgcccc gcgctggcat gcggagaagg gcccgcagcg catgaaggac acgtgcgcgg
     1561 cgatccccga attcaaggcc gacgccgacg cgctcgcaaa ggcgacgccg cccgagaagg
     1621 caaacgcgga cacgtggacg gcgagcacga aggcgctcgt cgactcggtc aacggtctcg
     1681 atccggtgtg ccaggcaaac gacgccgcga agttcgaaga ggcgtttcac aaggtccacg
     1741 agagcttcca cgccacgatg gccgccgccg gcatgcccat ggagaagcac gagggcggcg
     1801 agggcggcga gcacgagcac aagatgtaga cagccgccag cgcgctgctg ggacagacct
     1861 ggtttcgcgc gcggccgtgg tagaccgacg acatggccaa agatctgttg tggaaggtcg
     1921 gcgagcagta cgaaggctcg ccgctcgatc ctgcggattg cgatccagat ccgttcgtcg
     1981 aggtccgccg ctggctgaag ctcgccgtcg acggcggctt gccaacggcg aacgcgatga
     2041 cgctcgcgac cgtcgacgag cgcgatcggc cggccgcgcg cctcgtgctg ctcaaggagg
     2101 tcgacgaccg tggcttcgtg ttttacacga gctacgacag tcggaaggcg cgcgacctcg
     2161 cggcgcatcc gcatgcagcg ctcgtcatct actgggagcc actgcaccgc cagattcgca
     2221 tcgagggcag agtcgagaag atttcggccg gcgagtccga cgcgtacttc tcgacgcggc
     2281 ctcgcggcag tcagctcggc gccgtggcgt caccgcagtc cgaggtgctg ccgaatcgcg
     2341 aggcgctcga gcgcaaagtc gccgcgctgt cggcgaagta cggcgacggc ccggttccgc
     2401 gcccgacgag ctggggcggg tatcgcgtcg ttcccgatat gatcgagctg tggcagggcc
     2461 agccgtcgcg cttgcacgat cgcgtgcgct atcggctgct cgacggctcg tgggtcaaag
     2521 accggctcgc cccgtgatca cgcgcgcgcg atcaccgcaa cgtgcatcgc gcaacgcaat
     2581 ttgaacccga gcgactcgta gagctggatc gcgggcgcgt tcgacgcgta cgcgtgcagg
     2641 tatggtgtct cgccgcgctc gatgattcgc gtcgcgacga gcgccgacag ctcgcgagcc
     2701 agaccgcggc cgcgcacatc gggatgcgtg cacacgccgc tgacctccgt aaagccagcg
     2761 tgcttcatgc gctccccggc catcgcgacg agaacgccgc gttccttcac gccccagaac
     2821 tcgccgagca gcggggtgcg cgctgcgaac gggcccggtt tcgtcagcgt cgcgagcgcg
     2881 tgcatcgcgg ggaaatcgtc ggcggtgagg cgttcgatgt gcgcgtcagt cgtgcgcgtg
     2941 gccgtcagct tgtcggcgac catctgcaca cccggcgcgg tcgtcatcgc gacgatgccc
     3001 acgggtagca cgatcggatc tgcctgcagg agcaacagcg tgccgtcgcg cggcacgagc
     3061 tc
//
LOCUS       DASUFX010000513         7906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1054534, whole genome shotgun sequence.
ACCESSION   DASUFX010000513 DASUFX010000000
VERSION     DASUFX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054534"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFV99_20575"
     CDS             <1..474
                     /locus_tag="VFV99_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061780.1"
                     /translation="APLVAWYGAVEVVPQNQVLAAMRAAEDPDGVRRKAIIEPADAAG
                     LPPLAQPEMHEGALVSYEPDEIVVRVDAPREGIVVLNEIMFPGWHVEVDGKAATPLRA
                     NYLLRAVVVGPGTHTITWRFEPRRWRILVGSYLLALAVMLAAAVVPRRRRAPAAS"
     gene            complement(419..2992)
                     /locus_tag="VFV99_20580"
     CDS             complement(419..2992)
                     /locus_tag="VFV99_20580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5061781.1"
                     /translation="MRLLLAVVIAIGVASASPAQAATIHGLVFDDANGDGVPSAGEAG
                     VANAVVAFGVQRFVVTDAAGQFDIDVVDGARGIVWARVPDGFTPGPVWGRWDGAADID
                     LALHRLATPVRGPFSFVVASDTHLPITQEYITAADLALAATNATATDPAPAFFTILGD
                     ITQSNQEFDLVETALAGLEVPWIPVPGNHDWYDGGAAWFKHFGPDNYSFDIGDTHFVV
                     WNMAMNDTDIRTYLGAELQRVPRTMTIVALTHAPPSEPVIDALRELGVDYVLTGHAHS
                     NRVVDHGGLIELNTQPMLMGGLDFTPGGYRVITFDHGRLTSVHRSTVESPLVTIVSPS
                     RGACAPARGAALVVAAELDASTTNITARVDCASPIAMRAAGGWNWRAELPPLAPGPHT
                     IVVEANAQSGAGATTTLTVETCETPPPPLPGPAWPQLGGDATHVGTRPYEVVPPLVTR
                     WASVVGGHVLSASPVIAQGLVFVTVTDLGDGASGGVVALDLLTGAQRWRVPTPVQVRG
                     GLAIAGDTVVATQIDGLTLALDATTGAQRWRYELSTEFEPEAAALFAPPAVDHDDVLV
                     GNQRALAVLASASGTPLWSTDPVPAGRNSQSAAALAIGDGIVVGTFERAVGGLLAWDR
                     ATGKLLWSRNDFDTIGINASPVIAGDTIYTVSAADTVAALDLAGQLRWRTALDSKGFE
                     WGNATIGTPAYAQGVLVVPTLYRDLVALDALTGVELWRHAGVPSPLRTTHYRGARESG
                     FAAAPIITGDLVWAVDTAGELSALELHSGSVMWHTSLGVPVLAGLAASGDWLIAASYD
                     GTVRALVPTPAERPVPAPVDCTAPPPEPPPSAGCCDTTGSPASLLLALVVVVGLRRRR
                     A"
     gene            3059..3793
                     /locus_tag="VFV99_20585"
     CDS             3059..3793
                     /locus_tag="VFV99_20585"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5061782.1"
                     /translation="MLSSLSIVVPAFNEAASIAATVGDALRIGSSVARELEVLVCNDG
                     SRDDTGKIVGELAAGDVRIRLLDRPQNAGIEASMRALYANARYDWVFLMSADRQWPMA
                     SLVELAGAAERGADFVIGVRPASRKRAVYTPYRRLVSWGYERIVRALGAPGGDPGSIK
                     LARRELMNTAVASRGVFAEGERLIRAARSGARVVEVGVEFTRRGAGKATGARRDVVAR
                     ALVDAARVTTSLLVGWPRPKQPKSDV"
     gene            complement(3834..4703)
                     /locus_tag="VFV99_20590"
     CDS             complement(3834..4703)
                     /locus_tag="VFV99_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061783.1"
                     /translation="MFVLATACHYEPPAAGDDDGVDAAVSADGQEPDGPAANDPTACV
                     EWFTWNEQQPNLGQASWGTQLTDIARHIPAQYGDTYWFDEPITAGHETTHGIQAHLRN
                     YEAPPGHVNAFYVLGNKAAFVVEPNMRKSDIKPFIVTALRGPRYNLYLEQQTEWDDTP
                     LYVFDEWTAYVNGAEVGVGQVQAGIYTGEWTDAVMGPLEFTLYAIATAKAAKQKDPTY
                     YATNMQFRCFTAWNIKRAMALYEAGHNMTQFEWDTQEQYATKLRTMPEAEPLREVARE
                     LWGADWTQSVLGF"
     gene            complement(4812..5519)
                     /locus_tag="VFV99_20595"
     CDS             complement(4812..5519)
                     /locus_tag="VFV99_20595"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5061784.1"
                     /translation="MADRLPKRLFDRRVTSKVRVLLDDWLPPVVREWRPLNRVLARFV
                     HGPNFDIDFKQHAFHLSDREIAEAYDRLAQGGARFRDTDTTPAQIDAIVGAARGRVLE
                     VGCGNGMIAERLRLSAAHDVVAVDVTLGSAEETRRRAGCDVALAGLPDLPFPDDAFDT
                     VVCSHTLEHIPDLWRAAAELRRVGRRVLVVVPCQRYYRYTIDYHLHFFPNAGPLERLL
                     GGLATLIDGDWFVIADR"
     gene            5574..6599
                     /locus_tag="VFV99_20600"
     CDS             5574..6599
                     /locus_tag="VFV99_20600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5061785.1"
                     /translation="MSALRLGVIGYGYWGPNLVRNFALGPRTKVAAIAEQAPHRRQVA
                     QTAYPYIKIVEDAGALIADPDIDAVAIATPIFTHYDLAKRALEAGKHVLVEKPLTPSV
                     AQAEELAALAAKQGRVLMVDHTFVYTGAVKKIRDLVAKGELGKILYFDSVRINLGLFQ
                     PDFNVIWDLAPHDLTIMDYVLSQTVGTQARWVSAIGTSHYGRHENLAYVTVGFDESVL
                     AHVHVNWVAPVKTRRTIIAGDKKMIVWDDTSPVEPVKVYESSVDVQHIDKESAYALNV
                     QYRSGDVHSPKLDGKEALATMVQTFAAACLDGAASPSDASVGVRIVRMLEAAQKSLEQ
                     QGARIPL"
     gene            6596..7696
                     /locus_tag="VFV99_20605"
     CDS             6596..7696
                     /locus_tag="VFV99_20605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5061786.1"
                     /translation="MKVPFVDLVAWAKPSREEYLAAFAKIIDTGAYVGGPQVGEFEAA
                     FASYCGGAQAASVKTGTDALLLALRALGVRPGDEVITAANSFFATGEAIALAGAKPVL
                     ADVDDATLLIDVDDAARRVTAKTKCIIPVHLFGQVADMTRVHELARKTGIRVLEDSAQ
                     AHGATRGNWRAGSSGDAAAFSFYPTKNLGALGEGGAVVSNSASVIDEVKRLRDHGQAG
                     RHDHVEVAYNARLDSVQCACLSISLRRLDAANAARRTLAAQYRAKLAGKVRLVDEAPD
                     SKPVYHLMIVRVDAARRDAIRTAMGEAGVATAIHYPTPIHLQKAFAYLGQGPGSCPVA
                     EQSVKEMISLPMFPDMTSAQVDRVVEVLLASV"
     gene            7719..>7906
                     /locus_tag="VFV99_20610"
     CDS             7719..>7906
                     /locus_tag="VFV99_20610"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5061787.1"
                     /translation="MEHRPHVVVIGGGFGGLTAAKKLKHAPVNVTLVDRRNHHLFQPL
                     LYQVATAALAPADIAYPIR"
BASE COUNT         1282 a         2611 c         2747 g         1266 t
ORIGIN      
        1 gcgccgctcg tcgcgtggta cggcgcggtc gaggtcgtgc cgcaaaacca ggtgcttgcc
       61 gcgatgcgcg ccgccgaaga tcccgacggc gtgcggcgca aggcgatcat cgagccggcg
      121 gatgcggccg gcttaccccc actcgcgcag ccggagatgc atgaaggcgc gctcgtgagc
      181 tacgagcccg acgagatcgt ggtgcgtgtc gacgcgcccc gcgagggcat cgtcgtgctg
      241 aacgaaatca tgtttcctgg ctggcacgtc gaggtcgacg gtaaggctgc gacaccgctg
      301 cgtgcgaact acctgctgcg cgcggtcgtt gtcggtcccg gtacgcacac gatcacgtgg
      361 cgcttcgagc cgcggcgctg gcggatcctc gtcggcagtt atctgctcgc gctcgcggtc
      421 atgctcgccg ccgccgtagt cccacgacga cgacgagcgc cagcagcaag ctagccggtg
      481 aaccggtcgt gtcgcagcag ccggcggacg gcggtggctc cggtggtggc gcggtgcaat
      541 cgacgggggc tggcacgggg cgctccgctg gtgtcggcac gagcgcacgc acggtgccgt
      601 cgtaggacgc ggcgatcagc caatcgccgc tcgccgcgag ccctgcgagc acgggcaccc
      661 cgagcgacgt gtgccacatg acgctgccgc tatgcagctc gagcgcgctc agctcgcctg
      721 cggtgtcgac ggcccacacg agatcgccgg tgatgatcgg cgccgcggcg aagccggact
      781 cgcgtgcacc gcgatagtgc gtggtgcgca gtgggctcgg cacgccggca tggcgccaca
      841 gctcgacgcc ggtcaatgcg tcgagtgcga cgagatcacg atacagcgtt ggcacgacca
      901 ggacgccctg tgcgtaggca ggcgtgccga tcgtcgcgtt gccccactcg aaccctttgc
      961 tgtcgagtgc ggtgcgccag cgcagctgac cggcgaggtc gagcgctgcg acggtatccg
     1021 ctgcactgac cgtgtagatc gtgtcgcccg cgatcaccgg cgacgcgtta atgccgatcg
     1081 tgtcgaagtc gttgcggctc cacaacagct taccggtcgc acgatcccac gcgagcaagc
     1141 caccaaccgc gcgctcgaac gtgccgacga cgatgccatc gccgatcgcg agcgccgctg
     1201 cactctggct attgcggccg gcgggaaccg gatccgtcga ccacagcggt gtgccggatg
     1261 cgctggcgag cacggcaagt gcacgctgat tgccgacgag cacgtcatcg tggtcgaccg
     1321 ccggcggcgc gaacaaggcc gcggcttccg gttcgaactc ggtcgacagc tcgtaccgcc
     1381 agcgctgtgc gccggtcgtc gcatcgagtg cgagtgtcag gccatcgatc tgcgtcgcga
     1441 ccacggtatc gcccgcgatc gcgaggccgc cgcgtacctg cacgggcgtc ggcacgcgcc
     1501 agcgctgcgc accggtcaac agatcgagcg cgaccacgcc gccggacgca ccatcgccga
     1561 gatcggtgac ggtgacgaac acgaggccct gcgcgatgac ggggcttgcc gagagtacgt
     1621 gaccgccgac aaccgatgcc cagcgcgtga cgagtggcgg cacgacctcg tacgggcgcg
     1681 tgccgacgtg ggtcgcgtcg ccaccgagct gcggccacgc gggaccgggc aatggcggcg
     1741 gcggcgtttc gcaggtctcg acggtgagtg tcgtggtcgc gcccgcaccg ctctgcgcgt
     1801 tcgcctcgac gacgatcgtg tgcggacccg gcgcgagcgg gggcagctcg gcgcgccagt
     1861 tccagccgcc ggcagcgcgc attgcgatcg gcgacgcgca gtcgacgcgc gccgtgatgt
     1921 tcgtcgtgct ggcatcgagc tcggcggcga caacgagcgc ggcaccacgc gcgggggcgc
     1981 acgcaccacg cgacggcgac acgatcgtga ccagcgggga ctcgacggtg ctgcgatgca
     2041 ccgaggtcag gcggccgtga tcgaacgtga tgacgcggta gccgcccggc gtgaagtcga
     2101 gaccgcccat caacatcggc tgcgtgttca gctcgatcaa gccgccgtga tcgacgacgc
     2161 gattgctgtg cgcgtgaccg gtgagcacgt agtccacgcc gagctcgcgc agcgcatcga
     2221 tcacgggttc gctcggtggt gcgtgcgtga gcgcgacgat ggtcatcgtg cgcggcacgc
     2281 gctgcagctc ggcgccgaga tacgtgcgga tgtcggtgtc gttcatcgcc atgttccaca
     2341 cgacgaaatg ggtgtcgccg atgtcgaagc tgtagttgtc ggggccgaag tgcttgaacc
     2401 acgccgcgcc gccgtcgtac cagtcgtgat tgccaggcac cggaatccac ggcacctcga
     2461 gtcccgcgag cgcggtctcg acgaggtcga actcctgatt gctctgcgtg atgtcgccga
     2521 ggatcgtgaa gaaggcaggc gccggatcgg tcgccgtcgc attggtcgcg gcgagcgcga
     2581 ggtcggcggc ggtgatgtac tcctgcgtga tcggcaggtg cgtatccgac gcgacgacga
     2641 acgagaacgg gccgcgcact ggcgtcgcga gccgatggag cgcgagatcg atgtcggcgg
     2701 caccgtccca gcgcccccat accggcccgg gcgtgaaccc atccggcacg cgtgcccaga
     2761 cgatgccgcg cgcaccatcg acgacgtcga tgtcgaactg accggccgcg tcggtgacga
     2821 cgaagcgctg gacgccgaac gcgaccacag cgtttgccac cccggcctcg ccggccgacg
     2881 gcacgccgtc accgttggcg tcgtcgaaca cgagcccgtg aatcgtcgca gcctgggccg
     2941 gcgacgcgct cgccacccca atcgctatga ctacggcgag caggagccgc acgggctctc
     3001 attatgcggg acgaagcccc cgctggcacc agcggatttc ctatgatctg gagcgcgcat
     3061 gctatccagc ctctcgatcg tggtccccgc cttcaacgag gcggcatcga tcgcggccac
     3121 cgtcggcgac gccctgcgca tcgggtccag cgtcgctcgc gagctggagg tcctcgtctg
     3181 taatgacggt tcgcgcgacg ataccggcaa gatcgtaggc gagctggcgg cgggagacgt
     3241 ccgcatccgc ctcttggacc gtccgcaaaa tgccggcatc gaagcgtcga tgcgggcgct
     3301 gtacgcgaac gcgcggtacg actgggtgtt cctgatgtcc gcggaccgcc agtggccgat
     3361 ggcgtcgctc gtcgagctcg cgggcgcggc ggagcgcggt gccgacttcg tgatcggcgt
     3421 gcgccccgct tcgcgcaagc gcgcggtcta cacgccgtat cgccggctcg tgtcgtgggg
     3481 ctatgaacgg atcgtgcgcg cgctcggtgc gccaggtggc gatcctggat cgatcaagct
     3541 cgcgcggcgc gagctgatga acacggcagt ggcatcgcgg ggtgtgttcg cggaaggcga
     3601 gcggttaatt cgggctgcgc ggtcgggcgc gcgcgtcgtc gaggtcggcg tggagttcac
     3661 gcggcgtggt gcaggcaagg cgaccggagc gcggcgtgat gttgtcgcgc gagcgcttgt
     3721 cgatgccgcg cgcgtgacga cgtcactgct cgtcggctgg ccacgcccga agcagccgaa
     3781 gtccgacgta tgaggtggtt gtggctcgct accaccgtcg cgaagcgtcg cgcctagaac
     3841 ccaagcacgg actgcgtcca gtcggcgccc cacagctcgc gcgcgacttc acgaagcggc
     3901 tccgcttccg gcatcgtgcg cagcttcgtc gcgtactgct cctgtgtgtc ccactcgaac
     3961 tgggtcatgt tgtggccggc ttcgtagagc gccatcgcgc gcttgatgtt ccatgcggtg
     4021 aagcaacgaa actgcatgtt cgtggcgtag taggtcggat ccttctgctt cgccgccttc
     4081 gcggtcgcga tcgcatagag cgtgaactcg agcggaccca tcacggcatc ggtccattcg
     4141 ccggtgtaga tgccggcctg gacctggccg acgccgacct cggcgccgtt gacgtacgcg
     4201 gtccactcgt cgaacacgta gagcggcgtg tcgtcccact cggtttgttg ctcgagatag
     4261 aggttgtagc gaggaccacg cagcgccgtc acgatgaacg gcttgatgtc ggacttgcgc
     4321 atgttcggtt cgacgacgaa cgccgccttg ttgccgagca cgtagaacgc gttcacgtga
     4381 ccgggcggtg cttcgtagtt gcgtaggtgc gcctggatgc cgtgcgtcgt ctcgtggccg
     4441 gcggtgatcg gctcatcgaa ccagtaggtg tcgccgtatt gcgccggaat gtggcgcgcg
     4501 atgtcggtca gctgggttcc ccagctcgcc tggcccaaat tcggttgctg ctcgttccac
     4561 gtaaaccact cgacgcacgc cgtcggatcg ttggccgcgg gtccgtcggg ttcctggcca
     4621 tcggcggaga cggctgcatc gacgccatcg tcgtcgcctg cggcgggcgg ctcgtaatgg
     4681 cacgctgttg cgaggacgaa cacccctgcc aaccattgtt gacgcatagt caacaagtcg
     4741 cgccgcgcgc ccgttccggc aagatcggtg ggctgacagc cgctgagctg aagcgcgatt
     4801 cggcgcctcg ctcagcggtc cgcgatcacg aaccagtcgc cgtcgatcaa cgtagccaag
     4861 ccgccgagca ggcgttcgag cggacccgcg ttcgggaaga agtgcaggtg gtagtcgatc
     4921 gtgtagcggt agtagcgctg gcagggcacg acgacgagca cgcgcctgcc gacccggcgc
     4981 agctctgccg cggcgcgcca cagatccggg atgtgctcga gcgtgtgcga gcacacgacg
     5041 gtgtcgaacg cgtcgtctgg aaatggcagg tcgggcagac cggcgagcgc gacatcacag
     5101 ccggcgcggc ggcgggtctc ctccgcggaa ccgagcgtca cgtcgaccgc gacgacgtcg
     5161 tgcgctgcgc tcagccgcag ccgctcggcg atcatgccgt tgccgcagcc gacctcgagt
     5221 acgcggccgc gggcggcgcc gacgatcgcg tcgatctgtg cgggcgtggt gtcggtatcg
     5281 cgaaaccgcg cgccgccctg ggcgagccgg tcgtacgcct cggcaatttc ccgatcgctg
     5341 aggtgaaacg cgtgctgttt gaagtcgatg tcgaagttcg ggccatggac gaaccgcgcg
     5401 agcacgcggt tgagcggccg ccattcgcgc acgaccggag gcagccaatc gtcgagcagc
     5461 acgcgcacct tcgaggtgac gcggcgatcg aacagacgct ttggcaggcg gtcggccacg
     5521 gctcaccgta ccacccttga cgcacccccc gcggcatgtg acggtgcccg accgtgagcg
     5581 cgctgcgact cggcgtcatc ggttacggct actggggccc gaatctcgtc cgcaacttcg
     5641 cgctcggtcc gcgcacgaag gtggcggcga tcgcggagca ggcgccccat cgccggcagg
     5701 tcgcacagac cgcgtacccg tacatcaaga tcgtcgagga tgccggcgcg ctgatcgccg
     5761 accccgacat cgatgcggtc gcgatcgcga cgccgatctt cacgcactac gatctagcga
     5821 agcgtgcact cgaggccggc aagcacgtgc tcgtcgagaa gccgctgacg ccgagcgtcg
     5881 cgcaggcaga ggagctcgct gcactcgccg cgaagcaagg ccgcgtgctg atggtcgatc
     5941 acacgttcgt gtacacgggc gcggtcaaga agatccgcga cctcgtcgcg aagggcgagc
     6001 tcggcaagat cctctacttc gattccgtgc gcatcaacct cgggttgttc cagcccgact
     6061 tcaacgtgat ctgggatctc gcgccgcacg acctcacgat catggattac gtgctgtcgc
     6121 agaccgtcgg tacgcaggcg cggtgggtgt cggcgatcgg cacgtcgcac tacggccgcc
     6181 acgagaacct cgcgtacgtg acggtcggtt tcgacgagag cgtgctcgcg cacgtgcacg
     6241 tcaactgggt cgcaccggtg aaaacgcgcc gcacgatcat cgcgggcgac aagaagatga
     6301 tcgtgtggga cgacacctcg ccggtcgagc cggtgaaggt ctacgagagc tccgtcgacg
     6361 tgcagcacat cgacaaggag tcggcgtacg cgctcaacgt gcagtatcgc tccggggatg
     6421 tgcactcgcc gaagctcgac ggcaaggaag cgctcgcgac gatggtgcag acgttcgcgg
     6481 ccgcgtgcct cgacggtgcg gcatcgccgt cggacgcgag tgttggcgtc cggatcgtgc
     6541 gcatgctcga agctgcgcag aagtcactcg agcaacaagg cgcgaggatc ccgctgtgaa
     6601 agtaccgttc gtcgatctgg tcgcgtgggc gaagccgtcg cgcgaggagt atctggccgc
     6661 gttcgcgaag atcatcgaca ccggcgcgta cgtcggcggt ccgcaggtcg gtgaattcga
     6721 ggcagcgttt gcgagctact gcggcggtgc gcaggcagcg tcggtgaaga ccggcaccga
     6781 cgcgctattg ctcgcgctgc gcgcgctcgg cgttcgcccc ggcgacgaag tgatcaccgc
     6841 cgccaattcg ttcttcgcga ccggtgaagc gatcgcgctt gcaggtgcga agccggtgct
     6901 cgctgacgtc gacgatgcga cgctgctgat cgatgtcgac gacgccgcgc gccgggtgac
     6961 ggcaaagacg aagtgcatca tcccggtaca tctgttcggc caggtcgccg acatgacgcg
     7021 cgtgcacgag ctcgcgcgca agaccggcat ccgcgtgctc gaagattcgg cgcaggcgca
     7081 tggcgcgacg cgtggcaact ggcgcgccgg ttcgagtggt gacgccgccg cgttcagctt
     7141 ctatccgacg aagaacctcg gcgcgctcgg cgagggcggc gcggtagtct cgaacagcgc
     7201 gagtgtcatc gacgaggtga agcgcctccg tgatcatggc caggccggac gtcacgatca
     7261 cgtcgaggtc gcgtacaacg cgcgcctcga ttcggtgcaa tgcgcgtgtc tctcgatctc
     7321 gctccgccgc ctcgacgccg cgaacgccgc acgccgaacg ctcgccgcgc agtaccgggc
     7381 gaagctcgcg ggcaaggtgc ggctcgtcga tgaagcgccc gacagcaagc cggtctatca
     7441 cctgatgatc gtgcgcgtcg acgccgcacg tcgcgacgcg attcgcaccg cgatgggcga
     7501 ggccggcgtc gcaacggcga ttcattaccc gacgccgatc cacctccaga aggcgttcgc
     7561 ctatctcggg cagggaccgg gtagctgccc ggtcgccgaa caatcggtca aagaaatgat
     7621 ttcgctaccg atgtttccgg atatgacgag cgcgcaagtc gatcgcgtcg tcgaagttct
     7681 gctcgcatcg gtataaggcg tgcaaacatc taagctgcat ggagcatcgg ccccacgtcg
     7741 tggtgatcgg aggcggattc ggaggcctga ccgccgccaa gaagctcaag cacgctcccg
     7801 tcaacgtcac gctcgtcgat cggcgtaacc accacctgtt tcagccgctg ctgtaccagg
     7861 tcgcaaccgc agcgctcgct ccggccgaca tcgcatatcc gattcg
//
LOCUS       DASUFX010000514        10925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_222317,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000514 DASUFX010000000
VERSION     DASUFX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222317"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1756
                     /locus_tag="VFV99_20615"
     CDS             284..1756
                     /locus_tag="VFV99_20615"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5061788.1"
                     /translation="MLTAVIVGAGFSGLAAGVYLKRIGIQDFVILEKADRVGGTWREN
                     TYPGAACDVPSHLYSYSFEPNPNWSRAYGGQAEILAYLEHCADKYGLRPHLKFGQHVD
                     SARFDETTGTWHIATAAGERLEARALLLGNGALHIPSLPDIPGLDSFTGTRFHSARWD
                     HSYDFTNKRVAVIGTGASAIQFVPQIAPKVAHLDVYQRTAPWVVPKRDRTMSEAERWL
                     FEHVPATHWLRRTGLYWLMESRVVGFAYAPKVNEALEKVARYFLAKAVPDPELRAKVT
                     PNYRLGCKRVLISNEWYPALQRDNVDVITDGIETITPRGVRTKDGIEHEVDALVLGTG
                     FKVTEYLSSIRVIGRNGVELNDAWRTSLRNYLGINVSGFPNLFLLMGPNTGLGHNSMI
                     FMIEAQARYAAKAIEELRARKLAFIDVLPSTEQAFRAELSRKMKNTVWMTGCQSWYQT
                     PDGEVFLWPGATFDYWWRTRTVDLHAYEMRALQEPVRRAA"
     gene            1778..3358
                     /locus_tag="VFV99_20620"
     CDS             1778..3358
                     /locus_tag="VFV99_20620"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5061789.1"
                     /translation="MHRGMLLVCLAACGSNESGDDGPHPTPAPACTAADGGGSTTVTA
                     PNLVYTLADRYQEAWLASPAVADLDGDGTQEIIVPRHELLLVWHTDGSVAWRATLPGR
                     IWSSPVVADLVPSKPGLEVAVTSRDQIAAFDAQGQPLSGFPVTWRDEMRSLAAGDIDG
                     DGQLELVAVTSTPLAANNQKDIVIAVEMNGQIVSGFPPNTTGAAGCDTACYVTGGYDQ
                     NIALGDVDGDHIADLFITQDNAYMSLHAGTGRAFDAAAIFDNRTKFSGIRWMVDYALA
                     QQGYANDEAVDEQAHFTNTAPAIADLDGDGVGELIALGSIQNAAQDDREKGVGLFVAT
                     KNGTRPTAWLAPPRFPDYSGGLWDGTNNIVAITNQVSIADLDPAHPGPELVFAGFDGR
                     IHAVDASGQPLWQTTYTTSADVWTGGIAIADLSGDGSPELVLATYSPSGGDLVIYDAG
                     GNELHRVALGGRGAMPVPTIADVDNDGDLDIVLSLKDAVDKMKSVVVYEVSGSSGNCL
                     AWPTGRGNFRRDGFLPPR"
     gene            3482..4090
                     /locus_tag="VFV99_20625"
     CDS             3482..4090
                     /locus_tag="VFV99_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061790.1"
                     /translation="MKRTLGLVLAVALVPAIANAQSADPYDPQTPTPTTPDTTSTTTV
                     VTPPPQQPAPVVVVPPAQEPPRTTIVTQQPESYEVVDQWNAPVFATGALVFAGSYGAS
                     AIVAASSDHPGADRLYVPVVGPWLALNDWGDCPIDEPRCDTNTTHKVLLVADGVFQAG
                     GVITMITGLLSPTTHTVYQRTADATKVRVTPTHNGFAVVGRW"
     gene            complement(4092..5276)
                     /locus_tag="VFV99_20630"
     CDS             complement(4092..5276)
                     /locus_tag="VFV99_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061791.1"
                     /translation="MRCWLVVAALAGCSFEHGMDVGGGGDDVPPDVAPSLRDIPHVPA
                     THEMPGIGDYMFTNTTIDTTTGTIQPPAPEGVVVAMVMQEPSGDELLVIRAHAISIPA
                     ATAVTVTGTRPLVLIADTITIAGLLDGSAHASVPGPGGMGATAVTKTGRGSDGVHTGA
                     SQDSGGGGGAYATNGATGGASGTATSCGSELANGGAGGQSWGTPTLDVLAGGAAGGIG
                     SAVSCTPPPIGAGGGAIQVSAFTSLAITGAVNAGGGGGGMGLNCGTNDGSAASGGGSG
                     GAIYLDAPLIELAGVIAANGGGGGGGGSSPGTNGSVGGDGLPSTMYASGGAAGGSYGA
                     NGGRGGIGTAGPTAGGNHTCDGNAGGGGGSVGYIVMHAKAGGVSGTGVQSPAATLDTT
                     GF"
     gene            5352..6689
                     /locus_tag="VFV99_20635"
     CDS             5352..6689
                     /locus_tag="VFV99_20635"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5061792.1"
                     /translation="MDKPSLETRLDSAWLDAHWMPFTGNRQFKAHPRMFVGAEGSYYI
                     DTEGKKVFDGLSGLWCCGLGHGRREIAEAIAKTASTLDYAPAFQFGHPHSFALANKIK
                     ELTPAGLDYVFFTCSGSEAADTSLKMARAYWRAKGQASKRILVGREKGYHGVNFGGIS
                     VGGIVGNRKVFGQAVEADHLPHTMLAKNAFSRGMPAQGVELADELLRLIQLNDASNIA
                     AVIVEPFAGSAGVIPPPVGYLQRLRELCTQHDILLIFDEVITGFGRAGAFTGSEAFGV
                     TPDILNFAKQVTNGAQPLGGVVAKKEIYDTMMAAGGPEYMLEFPHGYTYSAHPVACAA
                     GVAALEVLQKENLIARVRELAPYFENAVHSLKGAKHITDIRNFGLAAGLTFAAVPGEP
                     AKRPYEIAMKCLAKGFYVRYGGDTIQLAPPFIVEKVEIDRLVEVLGDAINATA"
     gene            complement(6690..8201)
                     /locus_tag="VFV99_20640"
     CDS             complement(6690..8201)
                     /locus_tag="VFV99_20640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5061793.1"
                     /translation="MPNEVENQEQLFRLMVANVRDYAIFMLDPTGHIATWNQGAERIK
                     GYTAEEIIGKHFSVFYPRHDVEAGKCEMELRVAAETGRFEDENWRVRKDGTQFWANVL
                     ITAVHDDAGKLVGFAKVTRDLTERKRAEEETTARLAAERANRAKDEFLAMLGHELRNP
                     LAPILTALQLIKLRGEAPLKEHEVIERQVKHMMHLVDDLLDVSRITKGKLELRKKPID
                     MRSVLAKAIEIASPLLEQRGHHFGLDAPNWPVGVDGDEARLTQVVANLLTNAAKYTEP
                     RGRIELTLRERDDVVEVEVTDNGAGIDKQLLPRIFDLFVQGNRSAERTTGGLGIGLTL
                     VKQLVELHGGTVRVRSDGPGEGSTFTVRLPAVILDAPAHDQRPRRIAKASTPRRILLV
                     DDNEDALMLLAEALEAAGHTVKTATDAAEALDLIESFKPELAILDIGLPVMDGYELAT
                     KICSRFNGSSPRMFALTGYGQKADREKSREAGFSEHFVKPVDVKQLIERISSN"
     gene            8322..8699
                     /locus_tag="VFV99_20645"
     CDS             8322..8699
                     /locus_tag="VFV99_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061794.1"
                     /translation="MPSHKRSQEVDGKPTIELENHDQNVGQRESSHAGGKGGKPTIGP
                     ETHDANYGQRDTIDEEEELGVDEELDIEESRTSRATGEDIEESRGARANTDVEGSRGG
                     RNGDDVEPYLGETRVISDDSEVE"
     gene            complement(8736..10136)
                     /gene="hydA"
                     /locus_tag="VFV99_20650"
     CDS             complement(8736..10136)
                     /gene="hydA"
                     /locus_tag="VFV99_20650"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX5061795.1"
                     /translation="MTRTLIKNGTVVTASDTFAADVWLENGKVAALVQHGTKLGDADQ
                     TVDASGKYIIPGGIDCHTHMELPFGGTTASDDFDSGTVAAAHGGTTTIVDFAIQSKGS
                     SMRAGLDAWHAKAEGKAAIDYGFHMIMTEANPSTLEEMAGCIREGVTSFKMFMAYPGV
                     FLVDDQQIFRAMLRAGELGGLITMHAEIGLPIDVLVQRALSKGHTAPVYHALTRPEVA
                     EATGTERAIALAEMAKVPVYMVHLSAQRALERVMEARDRGLPAYAETCPQYLFCSEDD
                     LRGEPGNEWKGAGYVCTPPLRPKHHHDHLWRGLQNYDLQVVATDHCPFCMKDQKELGR
                     GDFSKIPNGMPGVETRMHLLWEGVTQGKLSMNRFVEITSTAPAKIFGMYGKKGTLAVG
                     ADADVVVWDPKREKVLGKDTLHMRVDYSPFEGHKVPGSPSQVFSRGELIVENDQWVAK
                     KKQGRGQFVRRGTFGL"
     gene            complement(10133..10522)
                     /locus_tag="VFV99_20655"
     CDS             complement(10133..10522)
                     /locus_tag="VFV99_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061796.1"
                     /translation="MSDRYEIRPGSSKPGQSRYATHSEVLLALKATAKQLGLVAKLPA
                     GRLGSGWRASIEGLTVAGDTKRVDDHTLERFHVVGPCDQNKLLAFAEHIAAIAGKQAV
                     IGETDDEFWFVDKPKAKSKAKGKGKRS"
     gene            complement(10527..>10925)
                     /locus_tag="VFV99_20660"
     CDS             complement(10527..>10925)
                     /locus_tag="VFV99_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5061797.1"
                     /translation="IGVYICYDRHFPEGARLLGLHGAEIVYNPSATVAGLSQYLWKLE
                     QPAHAVANGYFVGAINRVGTEAPWNIGKFYGNSYFVDPRGQIISCGSEDKDELVVGEL
                     EMDMIEEVRRTWQFFRDRRPDSYGVLTEDR"
BASE COUNT         1841 a         3725 c         3460 g         1899 t
ORIGIN      
        1 gctcgatggc gagctcgacc gcgagatcga acgccgactc tgggagctgg acgaggtcga
       61 cgaggcgctc aaccagctct ccgcctaacg gccatccacc aagcggcttc cgacaccggc
      121 ccatccaccg agcgattccg acaccttttc gaccatccac caacggccgg cgcgctgata
      181 cctctgcgcc ggccgttgga tgtttcggcc ggtggcccga actgccaagg ccctggcccg
      241 gatctctatt gaccattgtt caatacggca ctacggtgcg gtcatgctca ccgcagtcat
      301 cgtcggcgct ggcttttcgg gacttgctgc aggcgtctac ctcaagcgga tcggcatcca
      361 ggacttcgtg atcctcgaga aggcggaccg tgtcggcggt acgtggcgtg agaacacgta
      421 tcccggtgca gcctgcgacg tgccgtccca tctctactcg tactcgttcg agccgaaccc
      481 gaactggtcg cgcgcgtacg gtggccaggc cgagatcctc gcgtacctcg agcactgcgc
      541 cgacaagtat gggctcaggc cgcacctcaa attcggccag cacgtcgact cggcgcgctt
      601 cgacgaaacg acgggcacgt ggcacatcgc gaccgccgcg ggcgagcgcc tcgaagcgcg
      661 cgcgctcctg ctcggcaacg gcgcgctgca cattccatcg ctgcccgaca tcccaggcct
      721 cgattcgttc accggcacgc gctttcactc ggcgcgctgg gatcacagct acgacttcac
      781 aaacaagcgc gttgcggtga tcggtaccgg cgcgagcgcg atccagttcg tgccgcagat
      841 cgcaccgaag gtcgcgcacc tcgatgtcta tcaacgcacc gctccgtggg tcgtgccgaa
      901 acgcgatcgc acgatgagcg aggccgagcg ctggctgttc gagcacgtgc ccgcgacgca
      961 ctggctgcgc cgcaccggcc tgtactggct gatggagagc cgcgtcgtcg gctttgcgta
     1021 cgcgccgaag gtcaacgagg cgctcgagaa ggtcgcgcgc tacttcctcg cgaaggcagt
     1081 gccggatccc gagctgcgcg cgaaggtgac gccgaactac cggctcggat gcaagcgcgt
     1141 gctgatctcg aacgagtggt acccggcgct gcagcgcgac aacgtcgacg tgatcaccga
     1201 tggcatcgag acgatcacgc cgcgcggcgt gcgcacgaag gatggcatcg agcacgaagt
     1261 cgacgcgctc gtgctcggca ccggcttcaa ggtcaccgag tatctgtcat cgattcgcgt
     1321 catcggccgc aacggcgtcg agctcaacga cgcgtggcgc acgtcgctgc gcaattacct
     1381 cggcatcaac gtgtcgggct tcccgaactt gttcttgctg atgggcccga acaccggcct
     1441 tggccacaac tcgatgatct tcatgatcga ggcgcaagcg cgctacgccg cgaaggcgat
     1501 cgaggagctg cgcgcgcgca agctcgcgtt catcgatgtg ctgccgagca cggagcaagc
     1561 gttccgcgcc gagctgtccc gcaagatgaa gaacacggtg tggatgaccg ggtgccagag
     1621 ctggtaccag acgccggacg gcgaagtgtt cttgtggcca ggtgcgacct tcgattactg
     1681 gtggcgtacg cgcaccgtcg atcttcacgc ctacgagatg cgagcgctgc aagaacccgt
     1741 gcgacgcgct gcctgatcgc gataccgtgg atgcatcatg catcgcggca tgctgctcgt
     1801 ctgtcttgcg gcttgtggtt cgaacgagag cggtgacgac gggccgcatc caacgccggc
     1861 cccggcgtgc acggccgctg acggcggcgg atcgacaacg gtcacggcgc cgaacctcgt
     1921 ctatacgctc gccgatcgct accaagaagc gtggctcgca tcgccggcag tcgccgacct
     1981 cgatggcgac ggcacgcaag agatcatcgt cccgcgtcac gagctcttgc tcgtctggca
     2041 caccgacggc agcgtcgcgt ggcgcgcaac cctgcccggc cgcatctggt cgtcgccggt
     2101 cgtcgccgat ctcgtgccga gcaagccagg gctcgaagtc gccgtcacgt cgcgcgatca
     2161 gatcgcggcg ttcgatgctc agggtcagcc gctctccggt ttcccggtca cgtggcgcga
     2221 cgaaatgcgc tcgctcgccg ccggcgacat cgatggcgat ggccagctcg agctggtcgc
     2281 ggtgacgtcg acaccgctcg ccgcgaacaa tcagaaggac atcgtgatcg ccgtcgagat
     2341 gaacggccag atcgtcagtg gctttccgcc gaacacgacc ggggccgctg gctgcgacac
     2401 cgcgtgctac gtgacgggcg gctacgatca gaacatcgcg ctcggtgacg tcgatggcga
     2461 tcacatcgcg gacctgttca tcacgcaaga caacgcgtac atgtcgctgc acgccggcac
     2521 cggccgcgca ttcgacgctg cggcgatctt cgacaatcgc accaagttct ccggcatccg
     2581 ctggatggtc gactacgcac tcgcgcagca gggctacgcg aacgacgaag cagtcgacga
     2641 gcaagcgcac ttcacgaaca ccgcgcccgc gatcgccgat ctcgatggcg acggcgtcgg
     2701 cgaattgatc gcgctcggct cgatccagaa cgcggcgcag gacgatcgcg agaagggcgt
     2761 cgggttgttc gtcgcgacaa agaacggcac gcggccgacc gcgtggctcg cgccgccgcg
     2821 cttccccgac tacagcggtg gtctgtggga cggcacgaac aacatcgttg cgatcacgaa
     2881 ccaggtttcc atcgcggatc tcgatccggc gcatccgggt cccgagctcg tgttcgcggg
     2941 ctttgatggc cgcattcacg ccgtcgacgc cagcggccag ccgctctggc aaacgacgta
     3001 cacgacgagc gccgacgtgt ggaccggcgg catcgcgatc gcggatctgt cgggcgacgg
     3061 cagcccggag ctcgtgctcg cgacctacag cccgagcggc ggcgatctcg tgatctacga
     3121 tgccggcgga aacgagctcc atcgtgtagc gctcggtggg cgcggcgcga tgcccgtacc
     3181 gacgatcgct gacgtcgaca acgacggcga tctcgacatc gtgctgtcgc tgaaggacgc
     3241 ggtcgacaaa atgaaatctg tcgtggtcta cgaggtctcg ggctcgtcgg gaaattgcct
     3301 agcgtggccg acgggccgcg gtaatttccg acgtgacgga ttcttgccgc cgcgctgacg
     3361 cacgacgcac gtgcgccgtc gctcgtaaca actgcatacg atcgccgcgt atcgtgtgtt
     3421 ctacgcgtgg caaccctctt gcagaaatgc cgtagcgcaa ccctgcaaag aggacattcc
     3481 tatgaaacga acccttggcc tagtgttggc tgtcgcgctg gttcccgcta tcgcgaacgc
     3541 ccagagcgcg gacccgtatg atccgcaaac gccgacgccg acgacgccag acacgacatc
     3601 gacgacgacc gtcgtgacgc cgccgccgca acagccagcg ccggtggtcg tcgtgccgcc
     3661 cgcgcaagaa ccaccgcgca cgacgatcgt gacgcaacaa ccggagagct atgaggtcgt
     3721 cgaccagtgg aacgcgccgg tgttcgcaac cggtgcgctc gtgttcgccg gcagctacgg
     3781 cgcatctgcg atcgtcgctg cgagcagcga tcacccgggc gccgatcgac tctacgtgcc
     3841 ggtcgtcggt ccgtggcttg cgctcaacga ttggggtgat tgcccgatcg acgagccgcg
     3901 ctgcgatacg aacaccaccc acaaggtgct gctcgtcgcc gacggcgtgt tccaggccgg
     3961 cggcgtgatc accatgatca ccggcctcct gtcaccgacg acgcacacgg tgtatcagcg
     4021 tactgccgat gcgacgaagg tcagggtcac gccgacgcac aacggattcg ccgttgtcgg
     4081 tcgctggtaa ctcagaaacc ggtggtgtcg agcgtcgcgg ccggtgactg cacaccggtg
     4141 ccgctcacac cgccggcctt tgcgtgcatc acgatgtagc cgacgctgcc tcccccgccg
     4201 cccgcattgc cgtcgcacgt gtggttacca cccgccgtcg gacctgccgt gccgatgcca
     4261 ccgcggccac cgttcgcgcc gtagctgccg cccgctgctc cgccgctcgc atacatcgtc
     4321 gacggcaagc catcgccacc gacgctgccg ttcgtgcctg gcgacgagcc accaccgccg
     4381 ccgccaccgc cgttcgcagc gatcacgcct gccagctcga tcagcggcgc atcgagatag
     4441 atcgcgccgc ccgagccacc gccggatgct gcactgccat cgttcgtgcc gcagttgaga
     4501 cccatcccac cacctccacc gcccgcgttg accgcgccag tgatcgcgag cgacgtgaac
     4561 gcactgacct ggatcgcacc accaccggcg ccgatgggcg ggggcgtgca cgacaccgcc
     4621 gagccgatac caccggccgc accgcccgcg agcacgtcga gagtcggcgt gccccagctt
     4681 tggccacccg cgccaccgtt cgcgagctca ctgccgcagc ttgtcgcggt gccggacgct
     4741 ccgcctgtgg cgccgttcgt cgcgtatgca ccgccaccgc cgcccgagtc ttgtgatgcg
     4801 cccgtatgca ccccgtcgct accgcgcccc gtcttggtca ccgcggtcgc gcccatgcca
     4861 ccgggtcccg gtacgctggc gtgcgcactg ccatcgagca aaccggcgat cgtgatcgtg
     4921 tccgcgatca gcacgagcgg tcgcgtcccc gtgacggtta ctgcggtcgc cgccgggatc
     4981 gagatcgcgt gcgcgcggat cacgagcagc tcgtcgcccg atggctcctg catcaccatg
     5041 gcgaccacga caccctcagg tgcaggcggc tggatcgtgc cggtcgtcgt atcgatcgtc
     5101 gtgttcgtga acatgtagtc gccgatgccc ggcatctcgt gggtcgccgg tacgtgcgga
     5161 atgtcgcgca gcgagggcgc aacatccgga ggtacatcgt caccaccgcc gccgacgtcc
     5221 ataccgtgct cgaacgagca tccggccagc gcggcaacca cgagccagca gcgcacggcc
     5281 ctgatcgtac ctgaggttag ccaaacccga ttgcgaattc cgggggtgct cgctaggttc
     5341 gacccttcgc tatggacaag ccctcgctcg aaacccgtct ggattccgcc tggctcgacg
     5401 cgcactggat gccgtttaca ggcaatcgtc agttcaaggc ccacccccgc atgttcgtcg
     5461 gtgcggaggg gtcgtactac atcgataccg aaggcaagaa ggtcttcgac ggcctctccg
     5521 gcctgtggtg ctgcggcctc ggccacggtc gccgcgagat cgccgaagcg atcgccaaga
     5581 ccgcctcgac gctcgactac gcgccggcgt ttcagtttgg ccacccgcac tcgttcgcgc
     5641 tcgcgaacaa gatcaaggag ctcacgccag ccggtctcga ttacgtgttc ttcacgtgct
     5701 cgggcagtga agccgccgac acgtcgctca agatggcgcg tgcgtactgg cgtgcaaagg
     5761 gccaggcctc gaagcggatc ttggtcggcc gcgagaaggg ctatcacggc gtcaacttcg
     5821 gcggcatctc ggtcggcggc atcgtcggca accgcaaggt gttcggccag gccgtcgagg
     5881 ccgatcatct tccgcacacg atgctcgcga agaacgcgtt ctcgcgcggc atgcccgcgc
     5941 agggcgtcga gcttgccgac gagctgctgc gcctgatcca gctcaacgac gcgtcgaaca
     6001 tcgcagccgt gatcgtcgag ccgttcgcgg gctcggccgg cgtgattccg ccgcccgtcg
     6061 gttatctgca gcggctccgc gagctgtgca cgcagcacga cattctcctc atcttcgatg
     6121 aggtcatcac cggctttggc cgcgccggcg cgttcacggg ctccgaagca ttcggcgtga
     6181 cgcccgacat cctcaacttc gcaaagcagg tcacgaacgg cgcgcagccg ctcggcggcg
     6241 tcgtcgcgaa gaaggaaatc tacgacacga tgatggcggc cggcggtccc gagtacatgc
     6301 tcgagttccc acacggctac acgtactcgg cgcatccggt ggcatgtgcc gctggcgtcg
     6361 cagcgctcga ggtgctccag aaggagaacc tgatcgctcg cgtgcgcgag ctcgcgccgt
     6421 acttcgagaa tgcggtgcac tcgctcaagg gcgcgaagca catcaccgac attcgcaact
     6481 tcggtctcgc cgccggcctc acgttcgcgg cagtgcccgg cgagcccgcg aagcggccgt
     6541 acgagatcgc gatgaagtgc ctcgcgaagg gcttctacgt ccgctacggc ggcgatacga
     6601 tccagctcgc gcctccattt atcgtcgaga aggtcgagat cgatcggctg gtcgaggtgc
     6661 tcggcgacgc gatcaacgca accgcgtagt tagttcgagg agatccgctc gatgagctgc
     6721 ttgacgtcga ctggcttgac gaagtgctcg gaaaagccag cctcgcggct cttctcgcga
     6781 tcggccttct gaccgtagcc ggtcagcgcg aacatgcgcg gcgagctgcc gttgaaccgg
     6841 ctgcagatct tcgtcgctag ctcgtagccg tccatcaccg gtaggccgat gtcgaggatc
     6901 gcgagctcgg gcttgaacga ctcgatcagg tcgagcgctt cggccgcgtc ggtcgcggtc
     6961 ttgacggtgt gacccgcggc ttcaagcgcc tcggcgagca acatcagtgc atcttcgttg
     7021 tcgtcgacga gcagaatgcg gcgcggcgtc gacgccttcg cgatgcgccg cgggcgctgg
     7081 tcgtgcgcgg gcgcgtcgag tatcaccgcc ggcagccgca cggtgaacgt agagccttcg
     7141 cccgggccgt cgctgcgcac tcggacggta ccgccgtgca gctcgacgag ttgtttgacg
     7201 agcgtgaggc cgatgccgag accgccggtg gtgcgctccg cactgcgatt gccctgcacg
     7261 aacagatcga agatgcgtgg caacagctgc ttgtcgatgc cggcgccgtt gtcggtgact
     7321 tcgacctcga ccacatcatc gcgctcgcgc agcgttagct cgatgcgtcc gcgtggttcg
     7381 gtgtacttcg ccgcattcgt cagtaggttc gcaaccacct gggtgagccg cgcttcatca
     7441 ccgtcgacgc cgaccggcca gttcggcgca tccagcccga agtgatgccc ccgctgctcg
     7501 aggagcgggc tcgcgatctc gatcgccttt gcgagcacgc tgcgcatgtc gatgggcttc
     7561 ttcctgagct ccagcttgcc cttcgtgatg cgcgagacat cgagcaggtc gtcgaccaga
     7621 tgcatcatgt gcttgacctg gcgctcgatc acctcgtgct ccttgagcgg tgcttcgccg
     7681 cgaagcttga tcagttgcaa cgcggtgagg atcggcgcga gcggattgcg cagctcgtgc
     7741 ccgagcatcg cgagaaactc gtccttcgca cggttcgccc gctcggccgc aagtcgcgcg
     7801 gtggtctctt cctcggcgcg cttacgctcg gtgagatcgc gcgtgacctt cgcgaagccg
     7861 acgagcttgc cggcgtcatc atgcaccgcc gtgatcagga cgttcgccca aaactgagtt
     7921 ccgtccttac gcacgcgcca gttttcgtcc tcgaaccgac cggtctcggc cgcgacacga
     7981 agctccatct cgcacttgcc ggcctcgacg tcgtgccggg gatagaagac cgagaagtgt
     8041 ttgccgatga tctcttctgc cgtgtatccc ttgatgcgct cggcgccctg gttccacgtc
     8101 gcgatgtgac cggtcggatc gagcatgaag atcgcgtagt cgcgcacgtt cgcgaccatc
     8161 agccggaaga gctgttcttg gttctccact tcgttcggca caacgcggcg agcataccgg
     8221 atccgcgccg catgtccctt cggacatacc gtgcgcaatc gcctcacgtg cgcgcacgca
     8281 cggccgccat caggcgcagt gggtgcatcg ctcgtgacca aatgccttcg cacaagcgat
     8341 cccaagaggt cgacgggaaa ccgacgatcg agctcgaaaa ccacgatcag aacgtcggcc
     8401 agcgagaaag tagtcacgcg ggaggcaagg gcggcaaacc gacgatcggc cccgagacgc
     8461 acgatgcgaa ctacggccag cgcgacacga tcgacgagga ggaggagctg ggtgtcgacg
     8521 aagagctcga tatcgaagag agccgcacgt cgcgagcgac cggcgaggat atcgaggaga
     8581 gccgcggtgc gcgcgccaac acggacgtcg agggcagccg gggtggccgc aacggcgacg
     8641 acgtcgagcc ctacctcggc gaaacccgcg tcatcagcga tgacagcgag gtcgagtaac
     8701 gctgcgaaac gctttgatta caaacgaatc gcagcctaca ggccaaacgt gccgcgacgt
     8761 acgaattgcc cgcggccctg cttcttcttc gccacccact gatcgttctc gacgatgagc
     8821 tcgccgcgcg agaacacctg gctcgggctc cccggaacct tgtggccctc gaacggcgag
     8881 taatcgacgc gcatgtgcag cgtgtccttg ccgagcacct tctcgcgttt cgggtcccag
     8941 acgaccacgt cggcgtccgc accgaccgcg agcgtgccct tcttgccgta catgccgaag
     9001 atcttcgcgg gagcggtcga cgtgatctcg acgaaccggt tcatcgacag cttgccctgc
     9061 gtgacgcctt cccacagcag gtgcatgcgc gtctcgacgc ccggcatgcc gttcgggatc
     9121 ttcgagaagt cgccgcggcc gagctccttt tgatccttca tgcagaacgg gcagtgatcg
     9181 gtcgccacga cctggaggtc gtagttctgc agcccgcgcc acaggtgatc gtggtggtgc
     9241 ttcggacgca gcggcggcgt gcacacgtac cccgcgccct tccattcgtt gcctggctcg
     9301 ccgcgcaggt catcctcgct gcagaacaga tactgcgggc acgtctccgc gtacgccggc
     9361 aggccgcggt cgcgcgcttc catcacgcgc tcgagcgcgc gctgcgccga caggtggacc
     9421 atgtacaccg gcactttcgc catctcggcg agtgcgatcg cacgctcggt gcccgtcgct
     9481 tcggcgacct cgggccgcgt cagcgcgtga tagaccggcg cggtgtggcc cttcgagagg
     9541 gcgcgctgga cgagcacgtc gatcggcaga ccgatctcag cgtgcatcgt gatcaggccg
     9601 ccgagctcgc cggcgcgcag catcgcgcgg aagatctgct ggtcatcgac gaggaacacg
     9661 ccgggatagg ccatgaacat cttgaacgac gtcacgccct cgcgaataca gcccgccatt
     9721 tcctcgagcg tgctgggatt cgcttccgtc atgatcatgt ggaagccgta gtcgatcgct
     9781 gccttgcctt cggccttcgc gtgccacgcg tcgaggccag cgcgcatcga gctgcccttc
     9841 gactggatcg cgaagtcgac gatcgtcgtc gtaccgccgt gcgcggccgc gaccgtgccg
     9901 ctatcgaagt cgtccgacgc cgtggtgcca ccgaacggca gctccatgtg cgtgtgacag
     9961 tcgatgccgc ccggaatgat gtacttgccg ctcgcgtcga cggtctgatc cgcatcgcca
    10021 agcttcgtgc cgtgctgcac gagtgcggcg accttgccgt tctccaacca tacgtcggct
    10081 gcgaacgtgt cgctcgcggt gactacggtg ccgttcttga tgagcgtcct tgtcatgacc
    10141 gctttccttt cccttttgct ttcgacttcg ccttcggctt gtcgacgaac cagaactcgt
    10201 cgtcggtttc gccgatgacc gcctgcttgc cggcgatcgc cgcgatgtgc tcggcaaacg
    10261 cgagcagctt gttctggtcg cacggtccga cgacgtgaaa gcgttcgagc gtgtgatcgt
    10321 cgacccgctt cgtatcgccg gcgaccgtca acccttcgat cgacgcgcgc cagccgctgc
    10381 cgagcctgcc ggctggcagc ttcgcgacga gcccgagctg tttcgcggtc gcctttagcg
    10441 cgagcagtac ttcgctgtgc gtggcgtacc gcgactggcc gggtttcgat gagcccggcc
    10501 tgatttcgta ccggtcactc atcggcttag cgatcttcgg tgaggacgcc gtacgaatcg
    10561 ggacggcggt cgcggaagaa ctgccacgtg cggcggacct cctcgatcat gtccatctcg
    10621 agctcgccaa cgacgagctc gtccttgtcc tcgctgccgc acgagatgat ctgaccgcgc
    10681 ggatcgacga agtacgagtt gccgtagaac ttaccgatgt tccacggtgc ctcggtaccg
    10741 acgcggttga tcgcgccgac gaagtagccg ttcgcgaccg cgtgcgcggg ctgctcgagc
    10801 ttccacaggt actgcgagag ccccgcgacc gtcgcgctcg ggttgtagac gatctccgcg
    10861 ccgtgcaggc cgaggagccg cgcgccctcg gggaagtggc ggtcgtagca gatgtacacg
    10921 ccgat
//
LOCUS       DASUFX010000515         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_916469,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000515 DASUFX010000000
VERSION     DASUFX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916469"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFV99_20665"
     CDS             complement(<1..703)
                     /locus_tag="VFV99_20665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061798.1"
                     /translation="MPADSRREGCDSGLGPGHVRLDYTLAMPRVLFVLTLVVWATTAH
                     ADDRDKALKLFEDSDKAYKAGKFEKAAELLREAYGLYPEPELLYNLGRAQEGLGDMQA
                     AVDSYEEYLKVAKKIKDRAAIERRVETLKAQIAKQEEDARQRAEDERRRAEDEERRKR
                     EEEERKRNPPPPIDTRTPLEKYGPWITMGAGGAFMVTGVFFGVRASGTHDDAVATPIQ
                     RDAVELQASAERSATI"
     gene            650..2254
                     /locus_tag="VFV99_20670"
     CDS             650..2254
                     /locus_tag="VFV99_20670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061799.1"
                     /translation="MAGAEARVAPLPSRIGRHAVVGYIATGGMAELFLGKEPTGKPVV
                     IKRILPHLARQTTFVSMFIDEARIGSRAKHPNLVEVMELGQVGMDLFLVMEYLVGENL
                     AGLVRRLTKRKERISYGLCAYIIAEVCDGLHFAHELKDDESGDPLELVHRDVSPQNVF
                     VTYGGDVKLLDFGVATASQRLTQTASGEVKGKYAYMSPEQCRGEPLDRRSDIFSLGTV
                     LYELTTLRRLFKRPNELQVMRAITEDPIPRPSREIPDYPPALEEICVRALARDPEERY
                     ATAAEMREDLLKAMPQLTPDGDPHEAIASKFARIFSDRMTQKRELLERVRRGGELGEL
                     LAPEIDVEVEVPIVSHGDATPLSDMRRQTEPVKRKKRTRSRYTGFVIAFAVAIASGAG
                     IGAYLRTRSHAVHQKQEKLAVVQLPTPPAPDPSPVEVKVQGVEEVAPVDEYIVISVDT
                     DPDGVKVKVDGESRGATPIDVRVKRGETPLKVELAQTGFDTHQLDVIPDRDQRLYFAL
                     MKQQKKIVPVRTPPKTERKPGFRRFD"
     gene            2455..4203
                     /locus_tag="VFV99_20675"
     CDS             2455..4203
                     /locus_tag="VFV99_20675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061800.1"
                     /translation="MGNNDEDDTTASPVAAPSDEDSELCALVSIDGVVTTHVLPHFGM
                     IDIGRGSNCDIVIEHPSVSRHHATLQVKPLVITDAQSRNGTRVRGNSLAPGISVRLEI
                     GEAIQLGQATVLIHHKRLVEETPGLATARTVDALLRQLEIECARSARSGSPFAYVRVQ
                     MERGEISYEQLRATMRMTDVVADDHGSFQLLLPDTPSDQVAGAIARITGLLGQRNAEA
                     RIGVARYPYDGTTAEALVARVWEQLEGPQVRSATEMDAVRALIAQVAVSDVSVLIYGE
                     TGVGKELCAEMLHRQSRRSNKPFVKLNCSTISESLIESELFGHERGAFTGATAATQGL
                     LEAGDGGTVFLDEIGELPLPAQAKLLRVLEERVVRRVGSTVGRTLDIRFIGATNRMLP
                     EEIDAGRFRRDLYYRINGVTVTIPPLRERQGEIAGLARAFAAKPRTNSVPTDLEDDAL
                     AALQRHQWPGNVRELRNTIERAVLFAAGGPIKAEHLTLQEAQPPRRSSGVTIPIERLS
                     SSDITTLDDSRFGDGRSSDSRLADSIAEVERRRILDALERCGGNQTRAARMLGISRNT
                     LLSRLDAYGLPRPRKT"
     gene            complement(4173..>5215)
                     /locus_tag="VFV99_20680"
     CDS             complement(4173..>5215)
                     /locus_tag="VFV99_20680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5061801.1"
                     /translation="ALSHSDVLICSGTVIAPHAILTAGHCLDSELLDVAVGDTLAAAT
                     RQRALAAFPHPDFDANTLDHDIAILIVDPPLAVAPISISPTLDATVGSTIRAVGYGWT
                     VVNDTTPAVRRTGTSQIDTIDELRISVHAAPAQACEGDSGGPALFDAGSGERVIGVTS
                     SGDPTCTQFARYTRVDVHSDFVLDTVARTSAGGAGAGDRCWYDANCSVGTCLPATDDD
                     RLSFCAPSCVGGCPGDLECITVAGDARCRHPAPSPGAEGSACKSDGDCAGGLCLAPKD
                     SSSRVCTTRCFSDLPGFDCPGDETCQRASDAGEACFVKADEGWCNATRGDASPVLLLL
                     ALVQVLRGRGRP"
BASE COUNT          903 a         1720 c         1728 g          864 t
ORIGIN      
        1 cgatcgtcgc cgagcgctcg gcgctggctt gcagctccac tgcatcacgc tggatcgggg
       61 tcgcgacggc atcgtcgtgg gtacccgacg cgcgaacgcc gaagaacacg cccgtcacca
      121 tgaacgcgcc tccggctccc atcgtgatcc acggtccata cttctctagt ggcgtgcggg
      181 tgtcgattgg tggcggcgga ttgcgttttc gctcttcttc ctcacgcttg cgccgctcct
      241 cgtcctcggc ccggcgccgc tcgtcttcgg cgcgttgccg cgcgtcctcc tcttgcttgg
      301 cgatctgggc cttgagcgtc tcgacgcgac gctcgatcgc agcgcgatct ttgatcttct
      361 tcgcgacctt caggtattcc tcgtaggagt cgacggcggc ctgcatgtcg ccgaggccct
      421 cctgggcgcg gccgaggttg tagagcagct cgggctccgg atagaggccg tacgcttcgc
      481 ggagcaattc agcggccttc tcgaattttc cagccttgta ggccttgtcg ctgtcctcga
      541 agagcttcag cgccttgtcg cgatcatctg catgggccgt agtggcccac acaaccaacg
      601 tcaaaacgaa caaaacccgc ggcatcgcta gcgtataatc gagcctaaca tggccggggc
      661 cgaggccaga gtcgcacccc tcccgtcgcg aatcggccgg cacgcagttg tcggttacat
      721 cgcgaccggc ggtatggccg agttgtttct cggcaaggag cccaccggta agccggtcgt
      781 catcaagcgg atcctgcctc acctcgcgcg gcagacgacg ttcgtctcga tgttcatcga
      841 cgaggcgcgc atcggttcgc gcgcgaagca cccgaacctc gtcgaggtca tggagctcgg
      901 ccaggtcggc atggacctgt tcctcgtcat ggagtacctg gtcggcgaga acctcgccgg
      961 tctcgtacgc cgtctgacca agcgcaagga gcgcatctcg tacgggctat gcgcgtacat
     1021 catcgcggag gtctgcgacg gcctgcactt cgcgcacgag ctcaaggacg acgagagcgg
     1081 cgatccgctc gagctcgtgc accgcgacgt gtcgccgcag aacgtgttcg tgacgtacgg
     1141 cggcgacgtc aagctgctcg acttcggcgt cgcgacggca tcgcagcggc tcacacagac
     1201 cgcgagcggc gaggtcaaag gcaagtacgc gtacatgtct cccgagcagt gtcgcggcga
     1261 gccgctcgat cgacgaagcg acatcttctc gctcggcacc gtgctctacg agctgaccac
     1321 gctgcgccgg ctgttcaagc gaccgaacga gctgcaggtg atgcgcgcga tcaccgagga
     1381 tccgatccca cggccgtcgc gcgagatccc ggactatccg ccggccctgg aagagatctg
     1441 cgtgcgcgca ctcgctcgcg atccagagga gcggtacgcg accgctgcgg agatgcgcga
     1501 agacttgttg aaggcgatgc cgcagctgac gcccgacggc gatccgcatg aagcgatcgc
     1561 atcgaagttc gcgcgcatct tcagcgaccg tatgacgcag aagcgcgagc tactcgagcg
     1621 cgtgcgccgc ggcggcgagc ttggcgagct cctcgcgccc gaaatcgatg tcgaggtcga
     1681 ggtcccgatc gtcagtcatg gcgacgcgac gccgctgtcg gacatgcgcc ggcagaccga
     1741 gccggtgaag cggaagaaac gcacgcgcag ccgctacacc ggcttcgtga tcgcgtttgc
     1801 agtcgcgatc gccagcggcg ccggcatcgg cgcgtacttg cgaacgcgtt cgcacgcggt
     1861 ccatcagaag caggagaagc tcgcagtcgt tcagctcccg acgccgcccg ctccggatcc
     1921 gtcaccggtc gaggtgaaag tgcaaggcgt cgaggaagtc gcgccggtcg acgagtacat
     1981 cgtgatcagc gtcgacaccg atccggatgg tgtgaaggtc aaagttgacg gtgagtcacg
     2041 cggcgctact ccgatcgatg tccgagtgaa gcgcggcgaa acgccactca aagtcgagct
     2101 cgcgcaaacc ggattcgata cccaccagct cgacgtgatc ccggatcgcg atcagcggct
     2161 ctacttcgcg ctgatgaagc agcagaagaa aatcgtaccg gtgagaaccc caccgaagac
     2221 cgagcgcaaa ccaggcttcc gccggttcga ttagcggcat gtacttttgt gcatgttgtc
     2281 aggcgttttc gtagctagag gcccgggttg gctggcgatc cgcgaaccta gggtcgcact
     2341 gctgcaacag tgcgtttgtg cccttttggc cagatgggct tccgaacgtc agccatctcg
     2401 cgcttctatc accgcgcgct gatagcgccc cgatcgtatg cttggggagt cggaatgggg
     2461 aataacgacg aagacgacac gactgcgagc ccagtcgccg ctccctccga cgaagattcc
     2521 gagctgtgcg cactcgtcag catcgacggc gtagtcacca cgcacgtgtt gccccacttc
     2581 ggcatgatcg acatcgggcg cggttcgaat tgcgacatcg ttatcgagca cccatcagta
     2641 tcgcggcatc acgcaacgct gcaggtgaag ccgctcgtga tcaccgacgc gcagagccgc
     2701 aacggcacgc gcgtgcgggg caactcgctc gcgcccggca tctcggtgcg gctggagatc
     2761 ggcgaagcga tccagctcgg ccaggcgacc gtgctcatcc atcacaagcg gctcgtcgaa
     2821 gagacgccgg gcctcgcgac ggcacgcacc gtcgacgcgc tgctgcgcca gctcgagatc
     2881 gagtgcgcac gctcggcgcg gagcggatcg ccgttcgcgt acgtgcgcgt gcagatggag
     2941 cgcggcgaga tcagctacga gcagctgcgc gcgacgatgc ggatgaccga cgtcgtcgca
     3001 gacgatcacg gaagcttcca gctgctgttg cccgacacgc cgtcggatca agtagccggc
     3061 gcgatcgcgc ggatcaccgg cctgctcggc cagcgcaatg ccgaggcgcg gatcggcgtc
     3121 gcacggtatc cctacgatgg cacgactgcc gaggcgctgg tcgcgcgcgt gtgggagcag
     3181 ctcgagggac cgcaggtgcg gagtgcgacc gagatggatg cggtccgcgc gttgatcgcg
     3241 caggtcgcag tgtcggacgt ctcggtgctc atttacggcg agaccggcgt cggcaaggag
     3301 ctgtgcgccg agatgctcca ccgccaatcg cggcgctcga acaagccgtt cgtgaagctc
     3361 aactgctcga cgatcagcga gtcgttgatc gagagcgagc tgttcggcca cgagcgcggc
     3421 gcgttcaccg gcgcgactgc cgcgacgcag ggcctactcg aagccggcga tggcggcacc
     3481 gtcttcctcg acgagattgg cgagctgcca ctgcccgcac aagccaagct cttgcgcgtg
     3541 ctcgaagagc gcgtcgttcg tcgcgtcggc tcgacggtcg gccgcacgct cgacattcgc
     3601 ttcatcggcg ccacgaaccg catgctgccc gaagagatcg acgccggccg gttccgccgc
     3661 gatctctact accggatcaa cggcgtgacg gtcacgattc caccgctgcg cgagcggcag
     3721 ggcgagatcg caggtctcgc gcgcgcgttc gccgcaaaac cgcgcacgaa ctccgtgccg
     3781 accgatctcg aggacgacgc gcttgcagcg ctgcagcgtc accaatggcc cggcaacgtg
     3841 cgcgagctcc gcaatacgat cgagcgtgcc gtgctgttcg ccgccggcgg cccgatcaaa
     3901 gccgagcatc tgacgctgca ggaggcacag ccgccgaggc gcagttcggg cgtgacgatc
     3961 ccgatcgaac gcctgtcgtc gtcggatatc acgacgctcg acgattcgcg attcggcgat
     4021 ggtcgcagct ccgattcgcg gctcgccgac tcgatcgcgg aagtcgagcg ccgccgcatc
     4081 ctcgacgcac tcgagcggtg tggtggcaac cagacgcgtg ccgcgcgcat gctcgggatc
     4141 tcgcgtaaca cgctgctgtc gcggctcgat gcttacggcc tgccgcgccc gcgcaagacc
     4201 tgaacgagcg cgagcaagag cagcaccgga ctggcatcgc cgcgcgttgc gttgcaccag
     4261 ccttcgtcgg ctttcacgaa gcacgcctcg cctgcatcgc tcgcgcgctg gcacgtctcg
     4321 tcgccgggac aatcgaagcc gggcagatcg ctgaagcagc gcgtcgtgca aacgcggctg
     4381 ctgctgtctt tcggtgcgag acagagtccg ccggcgcaat cgccgtcgct cttgcacgcc
     4441 gagccctcgg cgccgggact tggtgcagga tgacggcagc gtgcgtcgcc agcgacggtg
     4501 atgcattcca ggtcgccggg acagccgccg acacacgatg gtgcgcagaa cgacaagcga
     4561 tcatcgtcgg tcgcgggcag gcaagtgcca accgaacagt tcgcgtcgta ccagcagcgg
     4621 tcgcctgcgc cggcgccacc tgcgctcgtg cgcgcgacag tgtcgagcac gaaatcgctg
     4681 tgcacgtcga cgcgcgtgta gcgtgcgaac tgcgtgcagg tcggatcgcc cgatgacgtc
     4741 acgccgatca cgcgttcacc actgccggcg tcgaacagcg caggtccgcc ggagtcgcct
     4801 tcgcatgcct gtgccggtgc ggcgtgcacc gagatccgaa gctcgtcgat cgtgtcgatc
     4861 tgcgacgtgc ccgtgcggcg cacggctggc gtggtgtcgt ttacaacggt ccagccgtag
     4921 ccgacggcgc ggatcgtcga gccgactgtc gcgtcgagcg tcggcgagat cgagatcggc
     4981 gcaaccgcga gcggcggatc gacgatcaga atggcgatgt cgtgatcgag cgtgttcgcg
     5041 tcgaagtccg gatgcggaaa cgccgccagc gcgcgttggc gggtggcggc ggcgagcgtg
     5101 tcaccgacgg cgacgtcgag cagctcggag tccaggcagt gaccggcggt aaggatcgcg
     5161 tgcggcgcga tcacggtgcc gctgcagatc agaacgtcgc tgtgcgagag cgcga
//
LOCUS       DASUFX010000516         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1332728, whole genome shotgun sequence.
ACCESSION   DASUFX010000516 DASUFX010000000
VERSION     DASUFX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332728"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1276
                     /gene="ald"
                     /locus_tag="VFV99_20685"
     CDS             173..1276
                     /gene="ald"
                     /locus_tag="VFV99_20685"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5061802.1"
                     /translation="MRIGVPKEIKTQEFRVGMTPAGVAMLVQRGHTVLVEQNAGSGSA
                     ISDEAFVKAGAKIVATREEVWAADMVVKVKEPIAPEFALMRKDLLLFTYLHLAAAHEL
                     GKELLNRGVNGVAYETIEPTPGDLPLLTPMSAVAGRMAVQAGATYLERERGGKGVLLG
                     GVPGVKRGRVTVIGGGVVGSNAVKMAVGLGANVTVLDVNLKTLAYLDDIYAGRVSTQY
                     SDPLSIERACVESDLVIGAVLLPGARAPRLVTEKMIKEMEAGSVIVDVSIDQGGCVAG
                     ARPTTHDHPTYVVHDVIHYMVANMPGAVPRTSTYALTNATIKYVITLADNGLEKAVAQ
                     VPHMVTGINTYKGTIPHSAVADALGEKYTPFRV"
     gene            1421..2011
                     /locus_tag="VFV99_20690"
     CDS             1421..2011
                     /locus_tag="VFV99_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061803.1"
                     /translation="MDALYGAAYRLTRNARDAEDLVQDSLLRAYKFWDSFQQDSNCKA
                     WLLRIVTNTFINEYQRKKRSREVLDAATAEQDATDGVLVHTTARDKQSPDQAMLQASV
                     SDDVQRALEQLPDDFRTAVILCDMQGLSYKEIADIMETPVGTVMSRLFRGRKLLAAAL
                     REFAVAEGYVKGENAKTEDTIDLQKYRAAKAREGSQ"
     gene            2008..2850
                     /locus_tag="VFV99_20695"
     CDS             2008..2850
                     /locus_tag="VFV99_20695"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5061804.1"
                     /translation="MSLCTTIDTLAMAYLDDELAAQERYELETHLTECASCRSTLERE
                     RADRSMIRRALAVPATPDLLRAKISRSLDEADRADSKEQRRRWSSYLLPSSAIAAAAA
                     AIALFVGGQMPASPKVANVAQDAVKLTSRSLPLEVQGASTGPWVQQHFAEDVRPPTFV
                     EPELQENDSQLLGARLAPNGINGHDAAMMQWQVRLPGKAPFLLFVVAVNGINDDEMRD
                     GTPVRVNDRTLFVFETKDQAGHAVPVVSYVAPNRLGWMFVAPELSANDLISLASRVNL
                     VAPQ"
BASE COUNT          561 a          938 c          984 g          486 t
ORIGIN      
        1 accagaaact atggaccggt gtcagcgtcg caggaaagct cgatcacgag ctcgtcgatc
       61 tcggcaccat catgatcccg gtcgcggagc agtagcgcat cgcgtcacgc gcatcatgtg
      121 agcggtgcac gttgatcttc ggagggcgcg agacgatgct gcgcccatcc acatgcgaat
      181 cggcgttccc aaagagatca agactcaaga atttcgcgtc ggcatgacgc ccgcgggcgt
      241 cgcgatgctc gtgcagcgcg gccacaccgt gctcgtcgag caaaacgcgg gtagcggctc
      301 ggcgatttcc gacgaggcgt tcgtcaaggc gggcgcgaag atcgtcgcga cacgcgaaga
      361 ggtgtgggcg gcggacatgg tcgtgaaggt caaagagccg atcgctcccg agttcgcgct
      421 catgcgcaaa gacctgctgc tcttcacgta ccttcacctc gcggcggcgc acgagctcgg
      481 caaggagctc ttgaaccgcg gcgtcaacgg cgtcgcgtac gaaacgatcg agccgacgcc
      541 cggcgatctt ccgctgctca cgccgatgag cgcggtcgct ggccgcatgg cggtgcaagc
      601 aggcgcgacg tacctcgagc gcgagcgcgg cggtaagggc gttctgctcg gcggcgtgcc
      661 gggcgtgaag cgcggccgcg tcaccgtgat cggtggcggc gtcgtcggct cgaacgcggt
      721 gaagatggcg gtcggtctcg gcgcgaacgt caccgtgctc gacgtcaatc tcaagacgct
      781 cgcgtacctc gacgacatct acgcgggtcg cgtgtcgacg cagtactcgg atccgctcag
      841 catcgagcgc gcgtgcgtcg aaagcgatct cgtgatcggc gcggtgctgc tgcccggcgc
      901 gcgtgcgccg cggctcgtca ccgagaagat gatcaaggag atggaagcgg gctcggtgat
      961 cgtcgacgtg tcgatcgatc agggcggctg cgtcgccggc gcgcgcccga ccacgcacga
     1021 ccacccgacc tacgtcgtcc acgacgtcat ccactacatg gtggcgaaca tgccgggcgc
     1081 ggtgccgcgc acgtcgacat acgcgctgac gaacgcgacg atcaagtacg tcatcacgct
     1141 cgccgacaac ggcctcgaga aggccgtcgc ccaggtgccg cacatggtga ccggcatcaa
     1201 cacgtacaag ggcacgatcc cgcattctgc ggtggccgat gcgctcggcg agaagtacac
     1261 gccgttccgc gtctaagtta gtgatttttc tcaggggaat cggccaggaa gcgcctggcc
     1321 attttctcgg gaacaatgtc ggcgtggagg caggtagaga acctgaatgc tcgccgtgag
     1381 gcaaaagcgc aacgagttcg agaggcaagc tctcgtgcac gtggacgcat tgtacggcgc
     1441 ggcctatcgc ctgacgcgga acgcgcgcga cgccgaggac ctggttcagg actcgctgct
     1501 gcgcgcgtac aagttctggg attcgttcca gcaggactcg aactgcaagg cgtggctgct
     1561 ccgcatcgtg acgaacacgt tcatcaacga gtatcaacgc aagaagcgct cgcgcgaggt
     1621 gctcgacgcc gcgacggccg agcaggacgc caccgatggc gtgctcgtgc acacgaccgc
     1681 tcgtgacaaa caatcgcccg accaggcgat gttgcaagca agcgtgagcg acgacgtgca
     1741 gcgcgcactc gaacaattgc ctgatgattt ccgtaccgct gtgattctct gcgacatgca
     1801 gggcctctcc tacaaagaga tcgctgacat catggaaacg cccgtcggca ccgtgatgag
     1861 ccgactgttc cgcggccgca agctgctcgc tgcagcactg cgcgagttcg cggtggcgga
     1921 aggctacgtg aagggcgaga acgcgaagac cgaagacacg atcgacttgc agaagtaccg
     1981 cgctgccaag gcgcgcgagg gatcgcaatg agcctttgta cgactatcga tacgctcgcg
     2041 atggcatatc tcgacgacga gcttgccgcg caggagcgtt acgagctcga aactcacctg
     2101 accgaatgcg cgagctgccg cagcacgctc gagcgcgaac gcgccgaccg cagcatgatt
     2161 cgccgcgcac tcgccgtacc ggcgacgccc gatttgctcc gcgcgaagat cagccgctcg
     2221 ctcgacgaag cggatcgcgc agattcgaag gaacagcgcc ggcgctggtc gtcgtacctg
     2281 ttgccgagct cggcgattgc agccgcagcc gcagcgatcg ctctgttcgt cggcggccaa
     2341 atgccggcgt cgccgaaggt cgcgaatgtg gcgcaggatg cggtcaagtt gacctcgcgg
     2401 tcgctgccgc tcgaggtcca gggcgcgagc accggtccct gggtgcagca gcacttcgcc
     2461 gaagacgtgc ggccgccgac gtttgtcgag cccgagctgc aggagaacga ctcgcagctg
     2521 ctcggcgcgc gtttggcacc gaacgggatc aacggccacg atgccgcgat gatgcagtgg
     2581 caggtgcggt tgcccggcaa ggcgccgttc ctcctgttcg tggtcgccgt caacggcatc
     2641 aatgatgacg agatgcggga cggaactccc gtgcgcgtca acgaccgcac gctgttcgtg
     2701 ttcgagacga aagatcaggc tgggcacgcg gtaccggtgg tctcgtacgt cgcgccgaac
     2761 cggctcggct ggatgttcgt cgcgcccgag ctgtcggcga acgacctgat ctcgctcgcc
     2821 agccgcgtca atctcgtcgc cccgcagtaa accgccgtcg gaatcgttct gaacggcctc
     2881 tcaaggcttc tataagaggc cccttctggg agcggcggtg gtaactcggt tactattggc
     2941 ggcaggggaa tgagccgccg catcctact
//
LOCUS       DASUFX010000517         5656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1332774, whole genome shotgun sequence.
ACCESSION   DASUFX010000517 DASUFX010000000
VERSION     DASUFX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332774"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..2312
                     /locus_tag="VFV99_20700"
     CDS             306..2312
                     /locus_tag="VFV99_20700"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5061805.1"
                     /translation="MTIRERLFGRKKDFRDPLIFHRVSLIAFFAWVGLGADGLSSSAY
                     GPEEAFRSLGEGHRYMALFLAIAVALTVFIISYGYSRIIEQFPQGGGGYVVASKHLGP
                     YPGLVSGAALLVDYVLTIAVSVASAGDAVFSFLPPEWHAYKLVAVLAVLAGLTVMNLR
                     GVKESILLLTPIFLVFLITHAIVIVGAIVTNVDEAGAVATTVSAQARSDVGVLGVGAM
                     AVIFMRAFALGGGTFTGIEAVSNGLGIMREPRVRTGKRTMVLMATSLSLTAGGILIAY
                     LLLDVDRAAADSAHAGQTLNAVLADLFAGHWTIGGLQVGKAFVVVTLVAEGALLIVAA
                     QAGFVDGPRVMSNMALDQWLPSRFASLSEQLTMHNGVYLMSGAAAAVLLYTDGDLRTL
                     VVMYSINVFLTFSLSNLAMIRHWWRVRHRERWRKSITIHAVGLILCAGILAITTIEKF
                     ESGGWVTLVITSVVILLCLWVKHHYRRVGKALARLSEELALDAVPASAPTPPELDPSK
                     PTAVVLAAGYGGFGIHTLLQIPRVFPNQFDQVVFVSVGVIDAGTFKGEQEMAALDRRV
                     TSDLDKYVELATKHMGWAAAHEHTLGTEAVAELERLCREVHLQFPRSVFFAGKLIFRH
                     EGWWQRVLHNETAHAVQRRLEFDGLTMVVLPIRMREAAIKRGGP"
     gene            2381..4177
                     /locus_tag="VFV99_20705"
     CDS             2381..4177
                     /locus_tag="VFV99_20705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061806.1"
                     /translation="MTTAAAEIGSSASSYEIFGKLARGGMAEIFLARAATGAGVSRFV
                     VLKRVLQERATDLQFLQMFLDEARLAAQLQHPNIAQVYDVGRLGDSYFFTMEYVHGVT
                     LRELMHGLEGHPMPVGAALAIAAGTAAGLHHAHERIGVDGHHLGIVHRDVSPSNLMVS
                     FEGHVKVVDFGIAKAASRTQETHSGTVRGKISYLSPEQAVGAYVDRRSDVFSLGIVMW
                     EMLTGQRLYKRDSDYASMAAVIDEPVPPLPPTVPQPLAMIVMRALAKRPEQRFQTAQE
                     MLETLEHTASSIGMPISTASLSRFVRDVFGPRPEPWAGLAAKDLRASVVTVNAEPLPQ
                     QLVVPVASEIDRKLATVIDLAATGLPVDPRQRMMIGNQPVMRTTPTMRPVQRGLSPLW
                     IGLFAFVLAVGLVIGARYYLRKNASSVPMDAGISITMAVTDYDAGTTAEPVAPVEIDA
                     AVTPSVLPTVTERDAGVAAAVEIEAGVPADAAVPAVAAVKPPANPPPRQKPRPRPEPK
                     PREPKPRPDPKPRPDPADDDAPKLSPDWQEFATCAQTNAANQPTSCVMVACRLQEDSK
                     ARKWFAKVPAGQRANVIATCKIEGVEVEPPKQ"
     gene            4237..4707
                     /locus_tag="VFV99_20710"
     CDS             4237..4707
                     /locus_tag="VFV99_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061807.1"
                     /translation="MRRIGVLAFTALAACAAQSNEPIQMTHDACAPLALVSSAATQLQ
                     LDGLAAGQAMWRDRGVPALGLRAGETLEVRFEDAAGPFHGLYDDSQGVIYINNDLTDP
                     QVLAIVIAHETGHAFGLPHVPKSERISVMNPGNLDTPPTAADQAEIVARWGECN"
     gene            complement(4747..>5656)
                     /locus_tag="VFV99_20715"
     CDS             complement(4747..>5656)
                     /locus_tag="VFV99_20715"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5061808.1"
                     /translation="LLTAAASSVLAGNADRSAITETDVHLALRSQLERKLLGLATRIE
                     TRQTWQDLVLPVDQFDLLIELVSRIRHRKQVLETWGFGDKVGRGVGVSALFSGPPGTG
                     KTMIAGLLANELRLDLYQVDLSRVVSKYIGETEKQLAALFEAAESGHAIILFDEADSL
                     FGKRTEVKSSNDRYANLEVNYLLQRMEAFKGISLLTTNHETGIDQAFQRRLAFHVRVP
                     MPDQAHRAMLWRSMIPEQAACESELALDDLAAEFVMTGGYIKNAVLRAAYLAADGGSP
                     INRGHLRRAARAEYEAMGKVSYHPAA"
BASE COUNT          966 a         1795 c         1860 g         1035 t
ORIGIN      
        1 agcaatcgcc gtaccgtcga cgcggcctcg acgttccaac atctcgcgtg tagcaatgcg
       61 atcgcggcgt gcaggtgcgt gcacgatcgg tgcgcgactg tcagcgcgcc gagtcgtcga
      121 gtatgcatac gcgacgtaac aacggagacg agagaccgca gtgcgagccc caccactcac
      181 cgatgttggt ggcggctacc gtcactgcga cactcgcggc agacaagtac ttggttcgca
      241 acgtcttgac agcggcttaa tgcggcgcgt gcacatcttg atctggaagg tggtacgccg
      301 atcgcgtgac gattcgtgag cgcctgttcg ggcgcaagaa ggattttcgc gatcctttga
      361 tcttccaccg cgtgtcgctc atcgcgttct tcgcatgggt cggactcgga gcagatggct
      421 tgtcgtcatc tgcgtacggc cccgaggaag cgttcaggtc gctcggcgag ggccatcgct
      481 acatggcgct gttcctcgcg atcgctgtcg cactgacggt gttcatcatc tcgtacggct
      541 acagccgcat catcgagcaa ttcccgcagg gcggcggtgg ttacgtcgtc gcgtcgaagc
      601 acctcggtcc ctacccgggc ctcgtgtccg gtgccgcact gctcgtcgac tacgtgctga
      661 cgatcgcggt gtcggtcgca tcggcaggcg atgcggtgtt cagcttcctg ccgccggagt
      721 ggcacgcgta caagctcgtc gccgtgctcg cggtgctcgc gggcttgacg gtgatgaacc
      781 tgcgcggcgt gaaggaatcg atcctcctgc tcacgccgat cttcctcgtg ttcctgatca
      841 cgcacgcgat cgtgatcgtt ggcgcgatcg tcacgaacgt cgatgaggcg ggcgctgtcg
      901 cgaccaccgt gtctgcgcaa gcacgatcag acgtcggcgt gctcggcgtt ggcgcaatgg
      961 cagtgatctt catgcgtgcg ttcgcgctcg gcggtggtac gttcaccgga atcgaggccg
     1021 tgtcgaacgg ccttggcatc atgcgcgagc cacgtgtgcg taccggcaag cgcacgatgg
     1081 tcttgatggc gacgtcgctg tcgctcaccg ccggcggcat cctcatcgcg tacctgctgc
     1141 tcgacgtcga ccgcgccgcc gccgattccg cacacgcagg ccagacgctc aatgcagtgc
     1201 tcgccgatct gttcgcgggg cactggacga tcggcgggct tcaggtcggc aaggcgttcg
     1261 tggtcgtgac actcgtcgcg gaaggcgcac tgttgatcgt cgctgcacaa gccggcttcg
     1321 tcgacggtcc gcgtgtgatg tcgaacatgg cgctcgatca gtggctgcct agccgcttcg
     1381 catcactgtc cgagcagctg acgatgcaca acggcgtcta cctgatgtcg ggcgctgcag
     1441 cggcggtgct cctctacacc gacggcgacc tgcgcacact cgtcgtcatg tactcgatca
     1501 acgtgttcct cacgttctcg ctgtcgaacc tggcgatgat ccgccactgg tggcgcgtgc
     1561 gccatcgcga gcgctggcgc aaatcgatca cgatccacgc ggtcggcttg atcttgtgcg
     1621 ccggcattct ggcgatcacg acgatcgaga aattcgaatc gggagggtgg gtcacgctcg
     1681 ttatcacgag cgtagtgatc ctgctgtgct tgtgggtcaa gcatcactat cgtcgcgtcg
     1741 gcaaggcgct ggcgcgactg tccgaggagc tcgcgctcga cgcagtacca gcgagcgcac
     1801 cgacgccgcc cgagctcgat ccgagcaagc cgaccgcggt cgtgctcgcg gctggctacg
     1861 gaggcttcgg catccacacg ctgctccaga tcccgcgcgt gtttccgaat cagttcgacc
     1921 aggtggtctt cgtttcagtc ggcgtcatcg atgccggcac gttcaaaggc gaacaggaga
     1981 tggcggcgct cgatcgccga gtcacatcgg atctcgacaa gtacgtcgag cttgcgacca
     2041 agcacatggg ctgggcggcc gcgcacgagc acacgctggg taccgaagcc gtcgccgagc
     2101 tcgaacgact gtgtcgcgaa gtccatttgc agttcccgcg ctcggtgttc tttgccggaa
     2161 aactgatttt tcgtcacgag ggctggtggc agcgcgtgct gcacaacgag accgctcatg
     2221 ccgtgcagcg gcgattggaa ttcgatggct tgacgatggt tgtgttgccg attcggatgc
     2281 gcgaggcggc catcaagcgc ggtggaccgt aacttctgtg gggtagcgaa cggcaaagta
     2341 ggcatgcagt gggggacgta gggggttaac attctcggaa gtgacgacgg cggccgcgga
     2401 aatcggctcg agcgcgagta gctacgagat ctttggcaag ctcgcgcggg gcgggatggc
     2461 cgagatcttt ctcgcgcgcg ccgccaccgg tgccggcgtc tcgcggttcg tcgtgctcaa
     2521 gcgcgtgctg caggagcgcg cgaccgatct gcagttcctc cagatgttcc tcgacgaggc
     2581 acgactcgct gcgcagctac agcacccgaa catcgcgcag gtctacgacg tcggccggct
     2641 cggcgattcg tacttcttca cgatggagta tgtgcacggc gtgacgttgc gcgagctcat
     2701 gcatgggctc gagggtcacc cgatgccggt cggtgccgcg ctcgcgatcg ccgccggtac
     2761 tgccgccggt ctgcaccacg cgcacgagcg gatcggcgtc gacggccatc acctcggcat
     2821 cgtgcatcgc gacgtgtcgc cctcgaacct catggtgagc ttcgaaggac acgtgaaggt
     2881 cgtcgacttc ggtatcgcga aggcggcatc gcgcacgcag gaaacgcact cgggcacggt
     2941 gcgcggcaag atcagctatc tgtcgcccga acaggccgtc ggtgcgtatg tcgaccggcg
     3001 cagcgacgtg ttctccctcg gcatcgtgat gtgggagatg ttgaccgggc agcggctgta
     3061 caagcgcgac agcgactacg cgtcgatggc tgccgtgatc gacgagcccg tgccgccgtt
     3121 gccgccgacg gtgccgcagc cgctcgcgat gatcgtgatg cgcgcgctcg cgaagcggcc
     3181 cgagcagcgc ttccagaccg cacaagagat gctcgagacg ctcgagcaca cggcgtcgtc
     3241 gatcggcatg ccgatctcga ccgcgtcgct ctcgcgattc gtgcgcgacg ttttcggacc
     3301 gcgccccgag ccgtgggcgg gccttgccgc gaaggacttg cgtgccagcg tcgtcaccgt
     3361 caacgccgag ccactgccgc agcagctcgt cgtcccggtg gcgagcgaga tcgatcgcaa
     3421 gctggcaacc gtgatcgatc tcgccgcaac gggactcccc gtcgatccgc gccagcgaat
     3481 gatgatcggc aatcagccgg tgatgcggac gacaccgacg atgcgcccag tgcagcgagg
     3541 gctatcaccg ctgtggatcg gtctgtttgc gttcgtgctc gcagtcggtc tcgtgatcgg
     3601 cgcgcgctac tacctgcgca agaacgcgtc gtccgtaccg atggacgccg gtatctcgat
     3661 cacgatggca gtcaccgact acgatgcggg aaccaccgcc gagcccgtcg cgcccgtcga
     3721 gatcgatgcg gccgtcacgc cgtcggtgct gccgacggtg accgaacgcg atgcaggtgt
     3781 cgccgcggcc gttgagatcg aggcgggcgt cccagccgat gccgccgtgc cggcggttgc
     3841 cgccgtgaag ccgcccgcga atccgccgcc gcgccagaag ccgcgaccgc ggcccgagcc
     3901 gaaaccgcgc gagccgaaac cccggcccga cccgaaacca cgtcccgacc cggcggacga
     3961 cgacgccccc aagctctcgc cggactggca agagttcgcg acctgcgcgc agacgaacgc
     4021 ggcaaaccag ccgacgagtt gcgtgatggt cgcgtgccgg ctgcaagaag acagcaaggc
     4081 tcgcaagtgg ttcgcgaagg tgccggcggg ccagcgcgcg aacgtgatcg cgacgtgcaa
     4141 gatcgaaggc gtcgaggtcg agccacccaa gcagtaagag aagctcgcag ttgatttcga
     4201 gttgcgccgc gcagcgcgat tgctaccgtc gacgccttgc gacgcattgg ggtcctcgca
     4261 ttcaccgcgc tcgcggcctg cgctgcgcag agcaatgagc caattcagat gacgcacgat
     4321 gcgtgcgcac cgctcgcgct cgtgtcgtcg gcggcgacgc agctgcagct cgacggcctc
     4381 gcggccggcc aagcaatgtg gcgcgatcgc ggtgtgccgg cgctcggttt gcgcgcaggc
     4441 gaaacgctcg aagtgagatt cgaggacgcc gccggaccgt ttcacggtct ctacgacgac
     4501 tcgcagggcg tcatctacat caacaacgac ctcaccgatc cgcaggtgct cgcgatcgtc
     4561 atcgcgcacg agactggtca cgcatttggt ctgccgcacg tgccgaagag cgagcgaatc
     4621 tcggtgatga atcccggcaa cctcgatacg ccaccgaccg ctgccgatca ggcggagatc
     4681 gtcgcccgct ggggcgagtg taactgagca ctcgctcgct ggcttcagca gcgtcggggc
     4741 gtgcggctat gccgccgggt gataggacac cttgcccatt gcctcgtact cggcgcgcgc
     4801 ggcacgccgc agatgtccgc ggttgatggg tgagccacca tcggcagcca agtaggcggc
     4861 gcgtagaacg gcattcttga tgtagccgcc tgtcatcacg aactcggcgg ccagatcatc
     4921 gagtgcgagc tcgctctcgc aggctgcttg ctcggggatc atcgaacgcc atagcatggc
     4981 gcgatgggct tggtcgggca tgggaacgcg tacgtgaaat gcgagccggc gctgaaaggc
     5041 ctggtcgatt ccggtctcgt ggttggtggt cagcagcgag atccccttga atgcttccat
     5101 gcgctgcaac aagtagttca cttcgagatt cgcgtagcga tcgttgctcg acttcacttc
     5161 ggtacgcttg ccgaacagcg aatccgcctc gtcgaataga atgatcgcgt gcccggattc
     5221 ggcggcttcg aatagcgccg cgagttgttt ctcggtttca ccgatatact tcgacacgac
     5281 gcgcgacagg tcgacctggt agagatcgag tctgagctcg ttcgcgagaa gtcccgcgat
     5341 catcgtcttg ccagtcccgg gtggtcccga gaacagcgct gaaactccaa cgccccgacc
     5401 gaccttgtcg ccgaagcccc aggtctcgag tacctgcttg cgatggcgga tccgagagac
     5461 gagctcgatg agcagatcga actggtcgac cggaagcaca aggtcttgcc aggtctggcg
     5521 cgtttcgatg cgggttgcga gtccgagcaa ctttcgttca agttgggatc gcagtgcgag
     5581 atgaacgtcg gtctccgtga tcgcagagcg atccgcgttt cccgcgagca ccgatgacgc
     5641 agcggcggtc agaaga
//
LOCUS       DASUFX010000518         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1194153, whole genome shotgun sequence.
ACCESSION   DASUFX010000518 DASUFX010000000
VERSION     DASUFX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194153"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFV99_20720"
     CDS             complement(<1..825)
                     /locus_tag="VFV99_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061809.1"
                     /translation="MLYPALGRTFQVAAVLSVVLAAARAQGDVPSLTDFVLAADKADP
                     GALKLPPPPTPQEIALDEAVTAGRHWRIETDRGPIHVWIPANYDAATAATIVFVHGYF
                     VTVDDAWTDYRLPQQFALSGTNAMFIVAQAPMTKRESLVWPSLTALVRTVKDSVDVPM
                     PTQRLVAVGHSGAYRTLAAWLPNPQLDTVVLLDALYGEFRFVPWLREGKNRRFVNIAY
                     ETDRYSDEMHRYLPSTVRVDGLPTTGFPDARILYAKTDVGHWQLVTDGVALPLALRA"
     gene            984..1886
                     /gene="eutC"
                     /locus_tag="VFV99_20725"
     CDS             984..1886
                     /gene="eutC"
                     /locus_tag="VFV99_20725"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX5061810.1"
                     /translation="MSRTRQLIASLRDALGSAEPLPPPPPLPDVPPPPARVYSPRPEK
                     RPFAAEAAKHTTALLGIGHVGTRYATDVVLQFQAELAVAHAAVGALLPEDWATQNGFI
                     GVKSRVTDHREFLLRPDLGRRLDDESLQIVRDKCVRNPDVQIVVADGLSAVACMGSGK
                     ALYEAVAKACQARGLSVGSPVCARFARVWLEDEIGQEVGAKVSMILLGERPGLGTGDG
                     LSAYVVHDPRCGKTDGDRNMISNIHARGTPIEDAARRLAAFAAAMIEQRTSGVTLDLA
                     RLGESAERAPRPPQVRQRLVERGS"
     gene            1883..2536
                     /gene="eutL"
                     /locus_tag="VFV99_20730"
     CDS             1883..2536
                     /gene="eutL"
                     /locus_tag="VFV99_20730"
                     /inference="COORDINATES: protein motif:HMM:NF011934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HEX5061811.1"
                     /translation="MILEPIVPTVLSVRRLPNADPAMIRAYGGDPERHRSLGLVTVDQ
                     DDPTYVALDEATKHAPVDVIFAKSFYAGAAHASGRLSGEILGVLGATEPEAIEHALDA
                     LLRCLKHDACFYDADGKGTVTVFPHVIASLGEYLSKEAGLAAGEPMAYLVAPPLEASI
                     ALDAALKAAEVRALKVMTPPTETNFAAAWLTGTLDECEAAAVAYAAAVVDVAAAPKR"
     gene            2533..3846
                     /locus_tag="VFV99_20735"
     CDS             2533..3846
                     /locus_tag="VFV99_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061812.1"
                     /translation="MKRLVVLVLALGCGDNLPDPGTPHSGGRLRLGWWVYADGTRQLE
                     TSWYYDSALRQRCVPSDWSDGNRYCAPATDEAVYTSASCTAALGRTPIDAAPAPYFAT
                     TFYLKGEPLRSRVFERGPQTDAPLTVFQKFNDGCYPTELGDGFAYFELGREITNLARL
                     RRGEPRGAGELAVVDDVSDDGLRVPVTFYDRALGVECTAAARANVDSVECAPNDSAIV
                     SYFHEVGCLEPELAITGTVPTTAKQYSPLTRCWSYYDVGTEVNAPPLYEALGGTCTSV
                     SPPNGSQFFLVAGLHQLQGVLRQREVTTRRLAAIDLVRDDQRFADPLLYDSALATECW
                     RDENDRCVPATDATIEPFFADSGCTMPLDLALVPQGECDAPTKFARKGDEYHPLGLWY
                     TRTIFWLSTGDTCGIYRPPTPFNAWTVGPAIDPTMFATATLTIDR"
     gene            3880..5181
                     /locus_tag="VFV99_20740"
     CDS             3880..5181
                     /locus_tag="VFV99_20740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5061813.1"
                     /translation="MIKNRTAILVLLTALNLLNYVDRMIVAAVLKKIKAPVIEGGLDL
                     SNSQAGLLATAFLLGYFITSPIFGARADKGARKGLIALGVAVWSIATVVTGFAQTFWL
                     MILARVFVGVGEASYATLAPTIIDDLTPPEQKGRALAIFYLAIPVGSALGYVVGGFIE
                     ARWGWRSAFFICGGPGLVLALSCLLIVEPERKLREAKGKIIDGLRTLAKLPLFRRAVL
                     GYCTYTAALGAFAYWAPTFIAERFPTELVRKTIDASGHEVIKQTALESANYYFGLATI
                     LSGIIGTLIGGRWADSTQRHHPVSVDTAFDDPVNKRGVNALLRICAIGMVWATPFAAA
                     AFLMPTPWMFFAAAFLAEIGLFLSTSPVNAIGLRAVPPELRASAMAAMIFAIHLFGDL
                     WSPTVLGFILDYVVATIAMMALPITFAWSAYLWWPRRSEAA"
     gene            5136..>6025
                     /locus_tag="VFV99_20745"
     CDS             5136..>6025
                     /locus_tag="VFV99_20745"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061814.1"
                     /translation="MVRLSVVAAEVGGRVSSGELDETADAGMQNAPLALAPGSHLGKY
                     RLDRILGEGGMGVVWAAHDPDLERAVAIKVLRYAQAAQQLRQRLLREARAMAKLKHPN
                     VLTVYEVGTVGDRDYIAMELVDGKSLDEWLALGPTQDEIWTAVLAAGRGLAAAHEAGV
                     VHRDFKPHNVLRSRGGRVLVTDFGLARGMLAADEAAMTPQIDLDPKITPFENTVEAKP
                     ATPIPTKSSPSRDSVLDSPLTQTGALIGTPAYMAPEQYSGAPPDPRTDQFAFCVTAWQ
                     ALTGERPFRGSSLDEMRRSA"
BASE COUNT          974 a         2041 c         2047 g          963 t
ORIGIN      
        1 cgcacgcagc gcgagcggca gcgcgacacc gtccgtgacg agctgccagt gaccgacgtc
       61 ggtcttcgca tagaggatgc gcgcatcggg gaaccccgtc gtcgggaggc cgtcgacgcg
      121 gacggtgctc ggcagatagc gatgcatctc gtcgctgtag cgatcggtct cgtacgcgat
      181 attgacgaag cggcggttct tgccttcgcg cagccacggc acgaagcgga actcgccgta
      241 gagcgcgtcg agcagcacga cggtgtcgag ctgtggattc ggtagccacg cggcgagcgt
      301 gcgataagcg ccggagtgac cgaccgcgac gagacgttgc gtcggcatcg ggacgtcgac
      361 gctgtctttc acggtgcgaa cgagcgcggt cagcgacggc cacacgagtg actcgcgctt
      421 cgtcatcggt gcttgcgcga cgatgaacat cgcgttcgtg cccgatagcg cgaactgttg
      481 cggcaagcgg tagtcggtcc acgcgtcatc gacggtgacg aagtagccgt gcacgaacac
      541 gatcgtcgcg gcggtggcgg cgtcgtagtt cgccggaatc cagacgtgga tcggcccgcg
      601 atccgtctcg atgcgccagt gccgacccgc ggtgacggcc tcgtcgagcg cgatctcttg
      661 cggcgtgggc ggcggcggga gcttgagcgc tcctggatcg gccttgtcgg cggcaagcac
      721 gaagtccgtc agtgacggaa catcgccctg cgctcgcgcc gcggcgagga cgacagagag
      781 gaccgcggcg acctggaagg tgcgaccgag ggcagggtac agcacgatcc aagtttcacc
      841 agattaccgt cggggagcca gcaaaaagct tcggtggtga tcggccgcaa aatgcgcagg
      901 gtcaggtagc tcgcgtacac gggcgaaacg gtgcgccgcc gcacgcttgc ccaccgcgtc
      961 ccgtcgtgat gacgtaggag cccgtgagtc ggacccggca gctgatcgct tcgttgcgcg
     1021 acgcgctagg cagcgccgag cctctgccac cgcctccccc acttccggat gtcccaccgc
     1081 caccggcacg ggtgtactcg ccccgccccg agaagcggcc gtttgcggcc gaggccgcga
     1141 agcacaccac cgcacttctc ggtatcgggc atgtcggtac gcggtacgcg accgacgtgg
     1201 tgctgcagtt ccaggccgag ctcgccgtcg cgcacgctgc ggtcggcgcg ctgctaccgg
     1261 aggactgggc gacgcagaac ggtttcatcg gcgtgaagtc gcgcgtgacc gatcaccgcg
     1321 agttcttgct gcgccccgat ctcgggcgga ggctcgatga cgagtcgctg cagatcgtcc
     1381 gcgacaagtg cgtgcgcaac cccgacgtcc agatcgtcgt cgccgacggc ctatccgcgg
     1441 tcgcatgcat gggctccgga aaggcgctct acgaagcagt cgcaaaggcg tgccaggcgc
     1501 gcggactctc ggtcggctcg ccggtgtgcg cgcgatttgc ccgcgtgtgg ctcgaagacg
     1561 agatcggcca agaagtcggc gcgaaggtct cgatgatcct gctcggcgag cgcccgggac
     1621 tcggcacggg cgacgggctg tcggcctatg tcgtccatga tccgcgctgc ggcaagaccg
     1681 acggcgaccg caacatgatc tcgaacattc acgcgcgcgg aacgccgatc gaagatgccg
     1741 cgcgtcgact cgcagcgttc gcggccgcaa tgatcgaaca gcgcacgagc ggcgtgacgc
     1801 tcgatctcgc acggctcggt gaatcggccg agcgcgcgcc gcggccaccg caggtgcgtc
     1861 agcgcctcgt ggagcgcggc tcgtgatcct cgagccgatc gtgccgacgg tgctgagcgt
     1921 gcggcggctg ccgaacgccg accccgcgat gatccgcgcg tacggcggcg atcccgaacg
     1981 ccatcgctcg ctcggactcg tgaccgtcga ccaagacgat ccgacctacg tcgcactcga
     2041 tgaagctacg aagcacgcgc cggtcgacgt gatcttcgcg aagagcttct acgcgggcgc
     2101 cgcgcacgcg agcgggcgac tgtcgggaga gatcctcggc gtgctcggcg cgacggagcc
     2161 agaggcaatc gagcacgcgc tcgatgcact gctgcgctgt ctgaaacacg acgcgtgctt
     2221 ctacgacgcc gacggcaagg gcaccgtcac cgtgttcccg cacgtgatcg cttcgctcgg
     2281 cgagtacctg tcgaaggaag ccggcctcgc ggcgggcgag ccgatggcat acctcgtcgc
     2341 gccgccgctc gaagcgtcga tcgcgctcga tgccgcgctc aaagccgccg aggtccgcgc
     2401 gttgaaagtg atgacgccac cgaccgagac gaacttcgca gcagcgtggc tgaccggcac
     2461 gctcgatgaa tgcgaagcgg cggccgtcgc gtatgcggcg gcggtcgtcg acgtagcagc
     2521 agcaccgaag cgatgaagcg cctggtagta ctcgtgctcg cgctcggctg cggtgacaac
     2581 ctgcccgatc cgggtacgcc acacagcggc ggccggttgc gactcggctg gtgggtgtac
     2641 gcagacggca cgcgtcagct cgagacgagc tggtattacg actccgcgct gcgccagcgc
     2701 tgcgtgccga gcgactggtc cgacggcaat cgctactgcg cacccgcgac cgacgaggcc
     2761 gtttacacga gcgcttcatg tacggcagcg ctcggccgca cgccgatcga cgcggcgccg
     2821 gcgccgtact tcgcgacgac gttctacttg aagggcgagc cgctccgctc gcgcgtgttc
     2881 gagcgcgggc cgcaaaccga cgcgccgctc acggtgttcc agaagttcaa cgacggttgc
     2941 tatccgaccg agctcggcga tggcttcgcg tatttcgagc tcggccgcga gatcacgaac
     3001 ctcgcaaggc tccggcgcgg cgagccgcgg ggcgctggcg agctcgctgt cgtcgatgac
     3061 gtcagcgacg atggtttgcg cgtgccggtc acgttctacg accgcgcgct cggtgtcgag
     3121 tgcaccgcgg cggcgcgcgc gaacgtcgac agcgtcgagt gcgcgccgaa cgacagcgcg
     3181 atcgtgtcgt actttcacga ggtcggctgc ctcgagcccg agctggcgat cacgggcacc
     3241 gtgccgacga ccgcaaagca gtactcgccc ctcacgcgct gctggagcta ctacgacgtc
     3301 ggcaccgagg tgaacgcgcc gccgctgtac gaggcgctcg gtggcacgtg cacgtcggtg
     3361 tcgccgccga acggctcgca gttctttctg gtggcgggcc tgcaccagct ccagggtgtg
     3421 ctccggcagc gcgaggtgac gacgcgccgg ctcgcggcga tcgatctcgt gcgcgacgac
     3481 cagcgcttcg ccgatccact gctctacgac agcgcgcttg cgaccgagtg ctggcgcgac
     3541 gagaacgatc ggtgcgtgcc cgcgaccgac gcgacgatcg aaccgttctt tgcggactcc
     3601 ggttgcacga tgccgctcga cctcgcgctc gtgccgcagg gcgagtgcga tgcgccgacg
     3661 aagttcgcgc gcaaaggcga cgagtaccat ccgctcggcc tgtggtacac gaggacgatc
     3721 ttctggctgt cgaccggcga cacctgcggt atttaccggc caccgacgcc gttcaacgcg
     3781 tggacggtcg ggcccgcgat cgacccgaca atgtttgcga ccgcgaccct gacgatcgac
     3841 cgctgatcgc acccctgata cagttgccgg cggggcgcga tgatcaagaa ccggacggcg
     3901 atcctcgttc tgctcaccgc gctcaatctc ttgaactacg tcgaccgcat gatcgtggcg
     3961 gcggtgctca agaagatcaa agcgccggtg atcgagggtg gtctcgacct gtcgaacagc
     4021 caagctgggc tgctcgccac cgcgttcctg ctcggctact tcatcacctc gccgatcttc
     4081 ggcgcgcgcg cggacaaggg tgcgcgcaag ggcctcatcg cgctcggcgt cgcagtgtgg
     4141 agcatcgcga ccgtcgtcac cgggttcgcc cagacgttct ggctcatgat cctcgcgcgc
     4201 gtgttcgtcg gcgttggcga agcgagctac gcgacgctcg caccgacgat catcgatgac
     4261 ctcacgccac ccgaacagaa gggccgcgcg ctcgcgatct tctacctcgc gattccggtc
     4321 ggctccgcgc tcggctacgt cgtcggcggc ttcatcgagg cgcgctgggg ctggcgctcg
     4381 gcattcttca tctgcggtgg gcccggcctc gtgctcgcgc tcagctgcct cttgatcgtc
     4441 gagcccgagc gcaagctgcg cgaggcgaag ggcaagatca tcgacggcct gcgcacgctc
     4501 gcgaagctgc cgctgttccg ccgcgcggtg ctcggctatt gcacgtacac ggcggcgctc
     4561 ggcgcgtttg catactgggc gccgacgttc atcgccgagc ggttcccgac cgagctcgtg
     4621 cgcaagacga tcgacgcgag cggccacgag gtcatcaagc agaccgcgct ggaaagcgcg
     4681 aactactact tcgggctcgc gacgattctc tccggcatca tcggcacgct catcggcggt
     4741 cgctgggccg acagcacgca acgtcaccat ccggtgagtg tcgatactgc attcgatgat
     4801 ccggtcaaca agcgcggcgt caacgcactg cttcgcatct gcgcgatcgg catggtgtgg
     4861 gcgacgccgt tcgccgcggc cgcattcctc atgccgacgc cgtggatgtt cttcgccgcg
     4921 gcattcctcg ccgagatcgg actgttcctg tcgacatcgc cggtcaacgc gatcggtctc
     4981 cgcgccgtgc cgcccgagct gcgcgcgagt gcgatggcgg caatgatctt cgcgatccac
     5041 ctgttcggtg atctgtggtc gccgaccgtg ctcggcttca tcctggacta cgtggtggcg
     5101 acgatcgcga tgatggcgtt gccgatcacg ttcgcgtggt ccgcctatct gtggtggccg
     5161 cggaggtcgg aggccgcgtg agcagcggcg agctcgacga gactgcagac gcgggcatgc
     5221 agaacgcgcc gctcgcactc gcgcccggca gccacctggg caagtatcgg ctcgatcgca
     5281 ttctcggcga aggcggcatg ggcgtcgtgt gggccgcgca cgatccggat ctcgagcgcg
     5341 ccgtcgcgat caaggtgctt cgctacgcac aagcggcaca gcagctccgc cagcgcctcc
     5401 tgcgcgaagc gcgcgcgatg gccaagctca agcatccgaa cgtgctcacc gtctacgagg
     5461 tcggcaccgt cggtgatcgc gactacatcg cgatggagct cgtcgacggc aagtcgctcg
     5521 acgaatggct cgcgctcggc ccgacgcagg acgagatctg gaccgccgtc ctcgccgccg
     5581 ggcgcgggct cgccgccgcg catgaagccg gcgtcgttca tcgcgatttc aagccgcaca
     5641 acgtgctgcg cagtcgcggc ggacgcgtgc tcgtcaccga cttcgggctc gcgcgcggca
     5701 tgctcgcggc agacgaagct gccatgacgc cgcagatcga tctcgatccg aagatcacgc
     5761 cgttcgaaaa caccgtggaa gcgaagccgg ccacgccgat cccgacgaag tcgagcccgt
     5821 cgcgcgactc ggtgctcgac tcgccgctca cgcagactgg cgcgctgatc ggcacgcccg
     5881 cgtacatggc accggagcaa tatagcggtg cacctccgga tccgcgcacc gatcaattcg
     5941 cgttctgcgt cacggcgtgg caagcgctca ccggcgagag gccgttccga ggctcgtctc
     6001 tcgacgagat gcgcaggtcg gcgag
//
LOCUS       DASUFX010000519         7143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1055478, whole genome shotgun sequence.
ACCESSION   DASUFX010000519 DASUFX010000000
VERSION     DASUFX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055478"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..3796
                     /locus_tag="VFV99_20750"
     CDS             56..3796
                     /locus_tag="VFV99_20750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061815.1"
                     /translation="MAQMARTGDRYRVLRQIGAGGMGIVYEAEDLERGQKVALKTIEY
                     DDVEKVYRLKREFRVLADLSHPNLVSLFDLVVDADSCFFTMELLDGTDLLTYLWNRPV
                     GDVIALASTTHSPMTAPSAPSVAAGQLPTPDGPTDGVSVEASPCDIAKLRAVLPQLAR
                     GLRALHAAGKIHRDIKPSNIRVTSDGRAVLLDFGLVAELERRRSDGIVGTVAYMAPEQ
                     CAGDVPLTAAADWYALGVVLFQALTGRLPFDGAPSRVLYEKQTRPASRPSQLVRGLPS
                     DLDELCAELLEREPTARLGGPQLLRRLGVSDGDSGRLASGTSRSRDGGCAGRDAELAA
                     LEGALAGLKQRRGALALVRAMSGMGKTTLVEHFLERVRVSDPDAVVLRGRCIDTEDVP
                     YKAIDHMIDELSEWWLGRTPREAEAILPRDASLLPTLFPVLERVPAIADAPRTKKVVD
                     PQTRRTLAFEALRETLRRLGDRHTVVLFLDDMQWVDRDTTTLLADLMRAPDPPPVLLV
                     LAARADESEPVLDLVKRMDVAPTIIDVGPLPEQAALALALSQLGDNSAETARRLVREA
                     DGSPLFLLELTRFLHGRSVDAVVGKGIDAMLAERVVGLGESARLVAEVVAVAGEPVTR
                     RVLGLVTALPNAELTRQLSLLRAERIVRMSGSRADDTIESYHARVRTSVLHQLSPERR
                     SKHHRTLATALSAQGSAEQLARHWYAAGDIENASAHAKRAGDEARAKLDFDLSARWYA
                     ISLEGPQWTEVERRALQTQLADALADAGRPREAAERFMLAANGADRATALELRRRAAG
                     SLLQSGYVAEGLELTRTVLEGVGLRMTKTPLRALFSMLVRRAWLRIRGLGFRRRALSE
                     ISQAELTRVDVCEGVSFGLALVDTFRSMDFAARFLHLALRLGEPWRISRALALEADLL
                     AATAKGTRAQRLLDKLEHLTQSVTDASAPTQLTTTRAFIDFFIHNRFRTALGVWTDAI
                     ASYRANVGRAGFELDTVSMFCCWCLYYMGEIGELSRRVPAMAEAADRNGNRFTTVTLR
                     SAFPIAWLVRLDPDAVEAGLDNAVASWTMPDGSYQLQHMFALCSRIELALYRQDPESV
                     TALIPGEVRRAERALLDRAPIQGLLLRTTLARHALACAAVAPANSPRRREALAIARAE
                     QRCCRKFPLPFAPYTAQVLEGVLFEIEGNTDAAIAAYRECIEGLDRHDTHLFAHAARH
                     RLGGLIGGEDGAKLRADVEAFFTREGLRDPAAVLAMLLPGPR"
     gene            complement(3797..5353)
                     /locus_tag="VFV99_20755"
     CDS             complement(3797..5353)
                     /locus_tag="VFV99_20755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061816.1"
                     /translation="MTGPSDPTFPSASDPSTELHAEEAYCSACNQSFPSGTTICPNDG
                     ARLIKLAAQRDELIGRVLDSRYEIRAPLGKGGMGTVYRGWQLSVDREVAIKVIHPKLS
                     NDRQAVKRFLREARLASRLSQPNIVNVYDFGQSDGVLYLVMELLRGHTLASELGQGRR
                     INHKRTTTVALQLCDALEAAHAQGIVHRDLKPSNIVILDDPPGRDLIKVLDFGLAKSL
                     VQDSGSVVTNTDALLGTPLYMAPEQIEGKVSDQRADLYSFGCILYEMLTGRPPFVDNA
                     VSAVLARHMHDVHIALPLHVPARLRDLIDKLLAKTPEDRLQTAGEMRRILEDVRDSVP
                     TLGKQATPLAIQIPDTPDTIPDISPELAETHAAPNPPTPVGRIVGVTSQTQPPTPGGA
                     KPKRYAIVAFFALIPLAVGAFVMFNVMNRSKQAANTGGSGAPTVKGNAFAGSGSDFDR
                     DRGSDDKPTLAQDPAPIVDAGVDAPAVEVVVPPPAADAGVKKKPEHRKPRVRSDAGAE
                     PDIDFLPTKK"
     gene            complement(5350..6606)
                     /locus_tag="VFV99_20760"
     CDS             complement(5350..6606)
                     /locus_tag="VFV99_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061817.1"
                     /translation="MTFREAVPVSLCVLAAACVKVPEFQPHDGAVGDGSDADASIGGP
                     LVHIEPAGLGAIVTAPRYEMRFSQDGSLFPYQLNVGANRENMVLGGSEHCADESGIGV
                     ALYPAWRANGLDQAGMGTPSLEIAADGPYVGQIRLSWSQSLSCGAGAGSLTGHTVYSF
                     FPDGRLARYDDVDNGPARDASDCSICTPNGQASSTFFLTSYTTLIADNNATSTDGNLA
                     TLDTYGEELMPGPGLSTCIAQRGTSIAFSWINAPTRVRVAAAPPTSQARTLAFVKDMF
                     TGGTLGASAGWATTTQMGISSEACGVLENRIAEFSVDDHQLLINGNSLGAAQVDGIYG
                     GYPQVSGYPVDFPVTLQPSGAQPRIPAGFAVWLYSQPIPQTMTLTHSGGHTGNWYYEQ
                     RVDQNSVVFWFNVSLEQGQTITITDS"
     gene            6660..>7143
                     /locus_tag="VFV99_20765"
     CDS             6660..>7143
                     /locus_tag="VFV99_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061818.1"
                     /translation="MHRVVIVILLAVAAMQAFADGGAADRAAAEAQTRAANSDFIGAA
                     AKYREAFKAEPRPDLMCNVGVAYYKAKDLPRAQRYLDQCLSIGTSLDRAFIDNVKKVL
                     AAVETKLAAGNFTPVDLLVQPPAATTNVEGGVPFDEPLLGSRRVWFPFGKYKLTVHAE
                     G"
BASE COUNT         1122 a         2474 c         2342 g         1205 t
ORIGIN      
        1 aaaactgcga agcgcaagcg gcgctaacgc acacgccagt agtacgatgg ggtgaatggc
       61 gcagatggcg cgtactggcg accgctatcg cgtgctgcgc cagatcggcg cgggcggcat
      121 gggcatcgtc tacgaggccg aggatctcga acgcggccag aaggtcgcgc tcaagaccat
      181 cgagtacgac gacgtcgaaa aggtctatcg gctcaagcgc gagttccgcg tgctcgccga
      241 tctcagccat cccaatctcg tctcgctgtt cgatctcgtc gtcgacgccg actcgtgctt
      301 cttcacgatg gagctgctcg acggtacgga cttgctcacg tacctgtgga atcgtcccgt
      361 tggcgacgtg atcgcgctcg cgagcacgac acactcgccg atgacggcac cgagcgcgcc
      421 gtcggtggcc gccggacaac taccgacgcc ggacggcccg acggacggcg tctcggtcga
      481 ggcctcgccg tgcgacatcg ccaagctgcg cgccgtgcta ccgcagctcg cgcgcggact
      541 tcgtgcgctc catgccgccg gcaagatcca ccgcgacatc aagccgtcga acatccgggt
      601 gacgagcgac ggtcgcgccg tgctgctcga cttcggtctc gtcgccgagc tcgagcgccg
      661 ccggtccgat ggcatcgttg gcaccgtcgc gtacatggcg cccgagcaat gcgccggtga
      721 tgtgccgctg acggcagccg ccgactggta cgcgctcggc gtggtgctgt tccaggcgct
      781 gaccggtcgc ctgccattcg acggcgcgcc gtcgcgcgtg ctctacgaga agcagacgcg
      841 gcctgcttcc cgcccgtcac agctcgtacg cgggctgccg agtgacctcg acgagctgtg
      901 tgccgagctg ctcgaacgcg aaccgaccgc gcggctcggt ggtcctcagc tactgcgccg
      961 gctcggtgtg agcgatggcg atagcgggcg cctcgcctcc ggaacgagcc gctcgcgcga
     1021 cggtggttgc gcggggcgcg atgccgagct cgccgcgcta gagggtgcgc tcgccggtct
     1081 gaagcagcgc cgtggtgctc tcgcgctcgt gcgcgcgatg tcgggcatgg gcaagacgac
     1141 gctcgtggag cacttcctcg aacgggtccg cgtatccgat ccagacgccg tggtgctgcg
     1201 cgggcgctgc atcgataccg aggacgttcc gtacaaggcg atcgatcaca tgatcgacga
     1261 gctctccgag tggtggctcg gccgcacgcc gcgcgaagcc gaagccatct tgccacgcga
     1321 cgcgtccctg ctaccgacgc tgttcccggt gctcgagcgc gtgcccgcga tcgccgacgc
     1381 gccgcgcacg aagaaggtcg tcgatcccca gacccggcga acgctcgcgt tcgaagcgct
     1441 gcgcgagacc ctgcgcaggc tcggcgaccg ccacacggtg gtgctgttcc tcgacgacat
     1501 gcagtgggtc gatcgcgaca ccacgacgct gctcgccgat ctgatgcgcg cgcccgaccc
     1561 gccgcccgta ctgctcgtgc tcgccgcgcg cgccgacgag agcgagccgg tgctcgacct
     1621 cgtcaagcgt atggacgtcg cgccgacgat catcgatgtc ggtccgctcc ccgagcaagc
     1681 cgcgctcgcg ctcgcactat cgcaactcgg cgacaacagc gcggagacgg cgcgccggct
     1741 cgtgcgcgaa gccgacggca gcccgttgtt cctgctcgag ctcactcgct tcctgcacgg
     1801 ccgcagcgtc gacgctgtcg tcggcaaagg catcgacgcg atgctcgccg aacgcgtcgt
     1861 tggcctcggc gagagtgcgc gtctcgtggc cgaggtcgtc gccgtcgcgg gcgaaccggt
     1921 cacgcgccgc gtcctcggcc tcgtcaccgc gctcccgaat gccgagctaa cacgccagct
     1981 gtcgttgctc cgcgccgagc gcatcgtgcg catgtccgga tcgcgcgccg acgacacgat
     2041 cgagtcctat cacgcgcgcg ttcgcacctc cgtactccac cagctctcac ccgagcgccg
     2101 cagcaagcac caccgcacgc tcgccaccgc gctatcagca caaggcagcg ccgaacagct
     2161 cgcgcgtcac tggtacgccg ctggcgacat cgagaacgcg tctgctcacg cgaaacgcgc
     2221 tggcgacgaa gcgcgcgcga aactcgactt cgatctctcc gcgcgttggt acgccatttc
     2281 gctcgaaggc ccgcagtgga cggaggtcga gcgccgcgca ctgcaaaccc agctcgccga
     2341 tgcgctcgcc gatgccggcc gtccgcgtga agcagccgag cggttcatgc tcgccgccaa
     2401 tggcgccgac cgcgccaccg cgctcgagct gcgtcgccgc gccgccggct cgctgctgca
     2461 gtccgggtac gtcgccgagg gtctcgagct gacgcgcacc gtgctcgaag gtgtcggctt
     2521 gcggatgacg aagacgccgc tgcgagcgct gttctcgatg ctcgtacgac gcgcatggct
     2581 gcgcattcgc ggcctcgggt ttcgccgccg tgcactgtcc gagatcagcc aggccgagct
     2641 cacccgcgtc gacgtctgcg aaggtgtgtc gttcggtctc gcgcttgtcg acacgttccg
     2701 ctcgatggat ttcgctgcgc gcttccttca cctcgcgctg cgcctcggcg agccatggcg
     2761 catctcgcgc gcgctcgcac tcgaagccga tctactcgcc gcgactgcca agggcacgcg
     2821 tgcccaacgc ctactcgaca agctcgagca cctcacgcag tccgtcaccg atgcatccgc
     2881 accgacgcag ctcacgacaa cccgcgcgtt catcgacttc ttcatccaca atcgcttccg
     2941 caccgcgctc ggtgtgtgga ccgacgccat cgcgagctat cgcgcaaacg tcgggcgcgc
     3001 cggcttcgag ctcgacaccg tcagcatgtt ctgctgctgg tgcctctatt acatgggcga
     3061 gatcggcgag ctgtcgcgcc gtgtgcccgc gatggccgag gccgccgatc gcaacggtaa
     3121 tcgcttcacc accgtcacgc tgcgcagtgc atttccgatc gcgtggctcg tccgactcga
     3181 tccagacgcc gtcgaggccg ggctcgacaa cgcggtcgcc tcgtggacca tgcccgacgg
     3241 cagctatcag ctccagcaca tgttcgcgct gtgctcgcgg atcgagctcg cgctctatcg
     3301 ccaagaccca gaatcggtga ccgcgctcat tcccggcgag gtgcgccgcg ccgagcgcgc
     3361 cctgctcgac cgcgcgccga tccagggttt gctgctccgc acgacgctcg cccgccatgc
     3421 gctcgcttgc gccgccgtcg cgcccgcgaa ttcaccacgc cgtcgcgaag ctcttgcgat
     3481 cgcgcgcgcg gagcagcgct gttgtcgcaa gttcccattg ccgttcgcgc cgtacacggc
     3541 acaggtgctc gagggcgtgc tcttcgagat cgagggcaac accgacgccg cgatcgccgc
     3601 gtatcgcgaa tgcatcgagg gcctcgatcg ccacgacacg cacctcttcg cgcacgccgc
     3661 acgccatcgg ctcggtggcc tcatcggcgg cgaggacggc gcgaagctgc gcgccgacgt
     3721 cgaggcgttc ttcacgcgag agggcctgcg cgatccagcc gcggtgctcg cgatgttgct
     3781 gccgggcccg cgctgatcac ttcttcgtcg gcaggaagtc gatatccggc tctgcgccag
     3841 cgtcggaccg cacgcgcggc ttgcgatgtt caggcttctt cttgaccccc gcatcggccg
     3901 cgggaggtgg cacgactact tctactgcag gcgcatcgac gcctgcgtcg acaatgggcg
     3961 ctgggtcctg cgccagcgtc ggcttgtcgt cgctgccgcg atcgcgatcg aagtcactgc
     4021 cgctgccggc aaacgcgttt cctttgacgg tcggcgcgcc gcttccaccg gtgttcgcag
     4081 cctgcttgct gcgattcatc acgttgaaca tcacgaacgc accgaccgcg agtgggatca
     4141 gcgcaaagaa cgcgacgatg gcgtagcgct tcggctttgc gccgccgggc gtcggcggct
     4201 gcgtctgtga cgtgacgccg acgatcctac cgaccggcgt tggcgggttc ggcgccgcgt
     4261 gggtctcggc gagctcgggc gagatatctg gaatcgtgtc gggcgtatcg gggatctgga
     4321 tcgcgagcgg ggtcgcctgc ttgccgagtg tcggcacgga atcgcgcacg tcttcgagaa
     4381 tccggcgcat ctcgccggcc gtttgcagcc ggtcttccgg cgttttcgcg agcagcttgt
     4441 cgatcagatc gcgcaatcgc gccggcacgt gcagcggcag cgcgatgtgg acgtcgtgca
     4501 tgtgtcgcgc gagcaccgcg ctcaccgcgt tgtcgacgaa cggcggcctg ccggtcagca
     4561 tctcgtagag gatgcagccg aacgagtaga gatcggcgcg ctggtcggag accttgccct
     4621 cgatctgttc gggcgccatg tagagcgggg taccgagcag tgcgtcggtg ttcgtaacga
     4681 ccgacccgga atcctggacg agcgactttg cgagaccgaa gtcgaggacc ttgatgagat
     4741 cgcggcccgg cggatcgtcg aggatgacga tgttcgacgg cttcagatca cggtgcacga
     4801 taccttgcgc gtgcgcggct tcgagcgcat cgcacagctg caaggcgacc gtggtggtgc
     4861 gcttgtgatt gatgcgccgc ccttgaccga gctcgctcgc cagcgtgtga ccgcgcaaca
     4921 gctccatcac gagatagagg acgccgtcgg actgaccgaa gtcatagaca ttgacgatgt
     4981 tcggctggct gagacggctc gcgaggcgcg cttcacgcag gaagcgcttc accgcctggc
     5041 gatcgttcga cagcttcgga tgaatgactt tgatcgcgac ttcgcgatcg accgacaact
     5101 gccagccgcg atagacagtg cccatgccgc ccttgccgag cggcgcgcgg atctcgtagc
     5161 gcgagtcgag tacgcgaccg atcagctcgt cgcgctgggc cgcgagcttg atcagccgcg
     5221 cgccatcgtt cgggcagatc gtcgtgcccg acgggaacga ttggttgcac gcagagcagt
     5281 acgcttcttc cgcgtgcagc tcggtcgacg ggtcggaggc cgagggaaac gttggatcgc
     5341 tcgggccggt catgaatcgg tgatggtgat ggtctgcccc tgctcgagcg acacgttgaa
     5401 ccagaatacg acactgttct ggtccacgcg ctgctcgtaa taccagttgc cggtgtgtcc
     5461 gccactgtgg gtcagggtca tcgtttgcgg aatcggctgg gagtacagcc acaccgcaaa
     5521 tccggccggg atccgcggct gcgcaccgct cggttgcagc gtgaccggga agtccaccgg
     5581 atagccgctc acttgcggat acccgccgta gatgccgtcg acctgcgcag cgccaagcga
     5641 gttgccgttg atcagcagct ggtgatcgtc gacggagaac tccgcgatcc gattctcgag
     5701 cacaccgcag gcctccgacg agatgcccat ctgcgtcgtc gttgcccagc cagctgacgc
     5761 cccgagcgtc ccgcccgtga acatgtcctt cacgaacgcc agggtgcgcg cctgggaggt
     5821 cggtggcgcc gccgcgaccc ggacgcgcgt cggtgcgttg atccacgaga acgcgatcga
     5881 cgtgccgcgc tgggcgatgc acgtcgagag gcccggcccc ggcatcagct cctcaccgta
     5941 cgtgtcgagc gtcgcgaggt tgccgtcggt gctcgtcgca ttgttgtccg cgatcagagt
     6001 cgtgtagctc gtgaggaaga acgtcgagct ggcttgcccg ttcggcgtgc agatgctgca
     6061 gtcgctcgca tcgcgcgcgg ggccgttgtc gacatcgtcg taacgcgcca accgtccgtc
     6121 ggggaagaag ctgtagaccg tgtgtccggt gagactgccg gcacccgcac cgcagctgag
     6181 cgactggctc cacgaaaggc ggatctgacc gacgtatgga ccatcggccg cgatctcgag
     6241 cgacggcgtg cccatgccgg cctgatcgag accgttcgcg cgccacgctg gatacagcgc
     6301 gacaccaatc ccggactcgt cggcgcaatg ctcggagcca ccgagcacca tgttctcgcg
     6361 gttcgcgccg acgttgagct gatacggaaa cagcgagccg tcctgactga accgcatctc
     6421 gtagcgcggc gcggtgacga tcgcaccgag acctgctggc tcgatgtgga caagcggtcc
     6481 gccgatcgat gcatccgcgt cactgccgtc accgactgcg ccgtcgtgcg gctggaactc
     6541 gggcaccttc acgcacgcgg cggcgagcac gcacagcgac accggtaccg cctcccgaaa
     6601 ggtcaccgag tgagtatccc tgcccgcgtg cagcgacgca acgatggtac gctcggcgcg
     6661 tgcatcgcgt agtcatcgtg atcttgctcg cagttgccgc gatgcaggca tttgccgacg
     6721 gtggcgcggc cgaccgtgcg gctgccgagg cgcaaacacg tgcggcgaac agcgacttca
     6781 tcggcgccgc cgccaagtat cgcgaagcgt tcaaagcaga gccgcggccg gatctgatgt
     6841 gcaacgtcgg tgtggcctat tacaaagcga aggatctgcc gcgcgcgcag cgctacctcg
     6901 accagtgcct gtcgatcggc acgtccctcg atcgcgcgtt catcgacaac gtcaagaagg
     6961 tgctcgccgc cgtcgagacg aagctcgccg cgggcaactt cacgccggtc gatctgctcg
     7021 tgcagccgcc ggccgccacc acgaacgtcg agggcggcgt cccgttcgac gaaccgttgc
     7081 tcggctcgcg tcgcgtgtgg tttccattcg gcaagtacaa gctcacggtg cacgccgagg
     7141 gtc
//
LOCUS       DASUFX010000520         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1750085, whole genome shotgun sequence.
ACCESSION   DASUFX010000520 DASUFX010000000
VERSION     DASUFX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750085"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFV99_20770"
     CDS             <1..339
                     /locus_tag="VFV99_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061819.1"
                     /translation="READYLQAPPAERAPNVLIDDQGRIQIYPALKSLGSTPVAVIQA
                     TNDSYVAAAEARQLLGPDTPTLRLYEVESSNHRFSGARDKMIEDVDDALQWIETSPRV
                     TARELRAPPT"
     gene            complement(320..>3446)
                     /locus_tag="VFV99_20775"
     CDS             complement(320..>3446)
                     /locus_tag="VFV99_20775"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5061820.1"
                     /translation="MNVSRRFIDYPVMTTLVMAAVVIFGAVGYFTLPVNELPNVDFPT
                     IGVSASLPGADPETMASAVAAPLENVFSTVSGIDSMTSSSGQGFTNITLQFRLDRNID
                     AAAQDVQAAISSAQRQLPRSMPSPPTFRKQNPADSPILFLALTSKTLPMTTLDRYAET
                     LLARQLSTLEGVAQVQVFGAAHYAVRVQADPAALAARGIGIDRLATAIQEANSNQATG
                     ALNGDDAKIIHTEGQLHSAAAFRRQIVAYRNGAPVRLEDVARVMDSTDNVRNGSWYRG
                     EPAITVAISRQPGSNTIEVIDEIKSVLPQFQASLPAGVDLSIRFDRSDSIRASIDDVQ
                     RALLIAGVLVVGVIFLFLRRASATIIPSLALPIAVVGTFAGMAAFGYSLDNLSLMALT
                     LSVGFVVDDAIVMLENIVRHMELGETPHTAAIKGSKEIGFTILSMTVSLAAVFIPIVF
                     MSGIVGRLLHEFAVTIVIAVLVSGVVSVTLTPMLAARFLRDSHGRKPGRFERVSESAF
                     NALQRAYERSLQWSLRHKPTIAWLFVASVVATVGLFLVSPQDFLPSDDTGILQISVQA
                     ANGTSYERMVEYGKQVADVVNSDPNALGAMLQVGSSGAGSSSANVRAMLKPLRDRPLS
                     AEEVARELRPKLAGIVGTNVFVTNPPALRIGGRSARSSYQYSLQGIDLDELQQVAVRL
                     EQKLETTPGFVGVNSDFDRAAPSVEVEIDRDRAAALGVAPQLIEAAMGYAFGGQQISQ
                     IYASNDQYQVILELLPELQRDQSSLKTLYLTSSSGKLVPLAAVTKATSGTIPQSVNHS
                     GSLPAITISFDLAEGYALSDAVRGIEQASREVAVPATITGTFQGTAGAFQETTANMGV
                     LLAIAIVVVYIVLGILYESFIHPLTILSGLPSAALGALVTLWLAGMPVTLYAFVGMVM
                     LIGIVKKNAIMMVDFALQKLRSEAGVSPERAVYEAALVRFRPIMMTTMAALVGTLPIV
                     FGSGMGADSRRPLGVCVAGGLLLSQLLTLYITPVLFVYLDRFGSRFASRSEQSVQRQA
                     PASPQTTLAEL"
BASE COUNT          617 a         1152 c         1132 g          545 t
ORIGIN      
        1 cgcgaagccg attatttgca ggcgccgccc gccgagcgcg cccccaatgt tctgatcgac
       61 gatcaaggcc ggattcagat ttatcccgcg ctcaagtcct tgggctcgac gccggtcgcg
      121 gtcatccagg cgacgaacga ctcgtacgtc gccgcggccg aagcgcgcca gctgctaggc
      181 cccgacacgc cgacgctgcg gctctacgag gtcgaatcga gcaatcaccg cttcagcggc
      241 gcgcgcgaca aaatgatcga agacgtcgac gacgccctgc agtggatcga aacttcgccc
      301 cgcgtcacgg cgcgagagct tagagctccg ccaacgtagt ctgcggcgag gcgggagctt
      361 gccgctgcac cgattgctcc gaccgcgaag caaagcgcga gccgaagcgg tcgaggtata
      421 cgaagagcac gggcgtgata tagagcgtca agagctgcga gagcagcaac ccgcccgcca
      481 cgcacacgcc gagcggccgc cgcgagtccg cgcccatccc cgagccgaac acgatcggca
      541 gcgtgccgac gagcgccgcc atcgtcgtca tcatgatcgg ccggaaccgc acgagcgccg
      601 cttcgtacac tgcgcgctcc ggtgacacgc cggcctccga gcgcaacttc tgcagcgcga
      661 agtcgaccat catgatcgcg ttctttttga cgatgccgat cagcatgacc atgccgacga
      721 acgcgtacag tgtcaccggc atcccggcga gccacagcgt gaccagcgca ccgagcgccg
      781 ccgacggcag cccggagaga atcgtgagcg ggtggatgaa gctttcatag aggattccga
      841 gcacgatgta gaccacgacg atcgcgatgg cgagcagcac gcccatgttc gccgtcgtct
      901 cctggaacgc acccgccgtg ccttgaaagg tgcccgtgat agtcgccggc accgcgacct
      961 cgcggctcgc ctgctcgatg ccgcgcaccg cgtcggagag cgcgtagcct tcggccaggt
     1021 cgaacgagat cgtgatcgcc ggcagcgagc ccgagtggtt gacgctctgc gggatcgtgc
     1081 cgctcgtcgc cttcgtgacg gcggcaagcg gcacgagctt gcccgagctc gaggtgagat
     1141 acagcgtttt cagcgagctc tggtcgcgtt gcagttcggg cagcagctcg aggatcacct
     1201 ggtattgatc gttcgacgcg tagatctgcg aaatctgctg accgccgaaa gcgtagccca
     1261 tggcagcctc gatcagctgc ggagcaacgc ccagtgcggc ggctcggtcg cggtcgatct
     1321 cgacctcgac cgacggcgcg gcgcggtcga aatcgctgtt gacgcccacg aaacccggcg
     1381 tcgtctcgag cttctgctcg agccgtactg ccacctgctg cagctcgtcg aggtcgatgc
     1441 cctgcagact gtattggtag ctcgagcgcg cgctccggcc gccgatgcgc agcgccggag
     1501 gattcgtgac gaacacgttc gtgcccacaa ttccggcgag cttcggccgc agctcgcgag
     1561 cgacttcctc ggccgacagg gggcgatcgc gcagcggctt cagcatcgcg cgcacgttcg
     1621 cgctgctcga ccccgcgccg ctcgagccca cttgcagcat cgcgccgagg gcgttgggat
     1681 cgctattgac gacgtcggcg acctgcttgc catactcgac catgcgctcg taggacgtgc
     1741 cgttcgcggc ctgcaccgat atctgtaaga tgccggtgtc gtcgctcggc aagaagtctt
     1801 gcgggctcac gagaaacaag ccgacggtcg ccacgacgct cgcgacgaac agccacgcga
     1861 tcgtcggctt gtggcgcagg ctccactgca ggctccgctc gtaagcgcgc tgaagcgcgt
     1921 tgaacgcgct ctcgctcacg cgctcgaacc ggcccggttt gcggccgtgc gagtcgcgca
     1981 agaagcgcgc ggcaagcatc ggcgtgagcg tcacgctgac cacgcccgac acgagcacgg
     2041 caatcacgat cgtcacggcg aactcgtgca gcagccggcc gacaatgccg ctcatgaaca
     2101 cgatcgggat gaacacggcg gccagcgaca cggtcatgga caggatcgta aagccgatct
     2161 ccttcgagcc cttgatcgcg gccgtgtgcg gcgtctcgcc gagctccatg tgcctgacga
     2221 tgttctcgag catcacgatc gcgtcgtcga cgacgaaacc gaccgacagc gtgagcgcca
     2281 tcagcgataa gttgtcgagg ctgtagccga acgcggccat gccagcgaac gtgccgacga
     2341 ccgcgatcgg caacgcgagc gacgggatga tcgtggcgga cgcgcgccgt agaaacagga
     2401 agatcactcc gacgacaagc acgcccgcaa tcagcaacgc ccgctgcacg tcgtcgatcg
     2461 acgcgcgaat cgagtcgctg cgatcgaagc ggatcgagag atccacgccc gcgggcagcg
     2521 aggcctggaa ctgcggcagc acgctcttga tctcgtcgat gacctcgatc gtgttcgatc
     2581 cgggctggcg gctgatcgcg accgtgatcg cgggctcgcc gcggtaccag ctgccattgc
     2641 gcacgttgtc ggtgctgtcc atgacgcgcg ccacgtcttc gagacgcacc ggcgcgccgt
     2701 tgcgatacgc cacgatctgg cggcggaacg ccgccgcgct gtgcagctgg ccttccgtgt
     2761 ggatgatctt cgcgtcgtcg ccgttcagcg cgcccgtggc ctggttgctg ttcgcttctt
     2821 ggatggctgt cgcgagccga tcgatgccga tgccgcgcgc cgcgagcgcg gccggatccg
     2881 cttgaacgcg cacggcatag tgcgcggcgc cgaagacctg cacctgcgcc acgccctcga
     2941 gcgtcgacag ctggcgcgcg agcagcgtct cggcgtagcg gtccagggtg gtcatcggca
     3001 acgttttcga ggtcagagcg aggaacagga tcggcgagtc ggcgggattc tgcttgcgga
     3061 acgtcggtgg gctcggcatc gagcgcggca gctgccgctg tgcagaggag attgcggcct
     3121 gcacgtcctg cgccgccgca tcgatattgc ggtcgagacg aaactgcagc gtgatgttgg
     3181 tgaagccctg gccgctcgac gacgtcatcg agtcgatgcc ggatacggtc gagaacacgt
     3241 tctcgagagg cgcggcaacc gcggacgcca tcgtctcggg gtcggcgccg ggcaagctcg
     3301 ccgacacgcc gatggtcggg aagtccacgt tcggcagctc gttcaccggc agcgtgaagt
     3361 aacccactgc gccgaagatc accaccgccg ccatcacgag cgtggtcatc acgggatagt
     3421 cgatgaagcg gcgcgagacg ttcatc
//
LOCUS       DASUFX010000521        13753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1750255, whole genome shotgun sequence.
ACCESSION   DASUFX010000521 DASUFX010000000
VERSION     DASUFX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750255"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV99_20780"
     CDS             <1..261
                     /locus_tag="VFV99_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061821.1"
                     /translation="GDTKVHMKDGLFQKGHEASFTTSLTVPEDIARKIFVHGDAAAGV
                     QAFLEGIITVEGDLAQVVAMQTVDPSAEQMKLARQIETVTAA"
     gene            complement(262..1161)
                     /locus_tag="VFV99_20785"
     CDS             complement(262..1161)
                     /locus_tag="VFV99_20785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5061822.1"
                     /translation="MRPGTYLTFSLLTLTSCFVGEATDEQLSSVDQGARVRGAIFTTL
                     PDGSAVNHNIYDAKTDVYLDGGPSGGSPVHAAALAAGDYYFQVTDPSGKTLLSTDDIA
                     CRRFHVDTSGVISAVVGACTHVSGSDRDYASLGALTVQLMPYADTPNPGGEYKVWITP
                     VAAYASSGRFHGFVPGSTKTDNFKVLESETPPPPPPSCCGNGQLDAGEQCDDGNTTNG
                     DGCSATCTTETPPPPQPCCGDGHIDSGEQCDDGNTTGGDGCSATCQLEVSTPPGGGDW
                     DSGQLCDGCVSTVQTNPEVIFVP"
     gene            1412..2236
                     /locus_tag="VFV99_20790"
     CDS             1412..2236
                     /locus_tag="VFV99_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061823.1"
                     /translation="MRSVVAVVCLVGAGCDVVWRLDDVSGGISDAPRAVDAPSDGDDP
                     CPWGPRVQISAGGGNHDPQLSEDGLELFFVRGASGTYDIWRAVRTDVSQPFPMGVEVA
                     ELSSSVEDTDPTLAAGGNTIVFKSTRGGTGNHAWLATRSGRGQPFTIPVMLTGVPATV
                     AGVDLSADGLTLYVDDGGKLVSVTRIAGSTTFMNEKTLTLERTQYPSVSADGLELYYN
                     GAGVVRRKRQTTNDMFDPASLVQIDDGAADPDLTFGGTRLVMTGAGIVYVRERCAP"
     gene            2293..2757
                     /locus_tag="VFV99_20795"
     CDS             2293..2757
                     /locus_tag="VFV99_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061824.1"
                     /translation="MKKALLAMVVVAAAGYGVYRWRAHEAPVAAKTDSNLVTNRLWID
                     HLPRGERDTIQVFAAITEEPIGVFEAASRWKGEFEFFRYEQHGDELRIFFPQSNTRDK
                     VRAKATECNRGGMDYCLELEGSSHGVKKYYSREGWEIGSTRDAEVLINKLAL"
     gene            2835..4031
                     /locus_tag="VFV99_20800"
     CDS             2835..4031
                     /locus_tag="VFV99_20800"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5061825.1"
                     /translation="MGLLRRSQGINVARKDRKYVGRAGAAQDIEVVAASGSELRDAQG
                     RTFVDFQMGWCVGNLGWNPPEILAHVRAFEGPTYVAPGVLYEPWAELAEMLVEASPAK
                     LTHAFRTVGGTEAVELAMQLAMRRTGRHKCVGLAGAYHGNSIATRSLGSSEGDAHLAG
                     FKQLEPPLDEAALDRLETILKHEDIAAFVMEPIVLNLGVLIPDTAFMNGLDELCHRYG
                     TLVIMDEIGTGMGRCGRLFASELFELDPDILTLGKAITSGVAALSAVLATDYVADVEL
                     DFFSTYGWHPVAVDAAIATQQYWREHRDVMLANIAERSAHLRQQLSLMEFPREPELRI
                     QGLAAAVDLGDAEYVAKLEERCREEGLLMFAEDEALVMFPALTITQNEMQEGLDILAE
                     CVRRGR"
     gene            complement(4028..4801)
                     /locus_tag="VFV99_20805"
     CDS             complement(4028..4801)
                     /locus_tag="VFV99_20805"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX5061826.1"
                     /translation="MGLEILGLLAASAGASYGWWKLQQRRERKFFLDIPRDPDLEVVM
                     SVAQHEASSRGHAYLWSLHLMHGLAQDENFVAAIGKLDGDATKFENHVQDELDKRKSS
                     HDEEAQQEGARVVGYAFAASRAQGRATTITDVWARLARTPSAKAAAAAAGFDAVALLF
                     VLVHGMREPSTELPDRTDVHIVLRNDDHTTQEFVVALLRDVFDHNDEDATTKMLETHR
                     QGRTVVGRYKLAAAHDKVATARRRAREADFPLWIGVEDC"
     gene            complement(4816..5151)
                     /locus_tag="VFV99_20810"
     CDS             complement(4816..5151)
                     /locus_tag="VFV99_20810"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5061827.1"
                     /translation="MLDDLDDSALDDLPFGVVCLNESMTVVRMNRTESEKSGIQRWRA
                     IGRDFFGDVAPGPTNRSLAEHVQAFRSGQGAPAIEHTFKRRAGEDPTKIELVRGRSAE
                     RVYLCIRRD"
     gene            5197..5589
                     /locus_tag="VFV99_20815"
     CDS             5197..5589
                     /locus_tag="VFV99_20815"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5061828.1"
                     /translation="MSRDSAGKQDDGSAPLVAIAERWLACFDRRDLDGLLALYADDAT
                     HTSPKIRVRHPETGGLLHGKAAMRAWWQDSFDRLPSMRYVPTALTADRERVFMEYVRH
                     VDGEPDMPVAEVLEVVNGRIVASRVFHG"
     gene            5582..6328
                     /locus_tag="VFV99_20820"
     CDS             5582..6328
                     /locus_tag="VFV99_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5061829.1"
                     /translation="MAERLHDWLAQRPFALAMSSGFFSFFAHTGMLSSLTRKGFAPNL
                     VAGASAGALVGGAWAAGLDAQSLQERLVALRRADFWDPAPGAGLLAGKKFDRILRDLL
                     PVTAMTACRVPVKVSAFDIASRRTTVLHDTDLAHAIRASCAVPALFHPVWIDRRAYWD
                     GGILDRPGLAGIPAGERVLFHHIASKSPWRTRLPVPRRAGMVTLVIDGLPRSGPFKLD
                     AGRRALELARDATLRALDAPIVDDVVRVAA"
     gene            complement(6325..7404)
                     /locus_tag="VFV99_20825"
     CDS             complement(6325..7404)
                     /locus_tag="VFV99_20825"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX5061830.1"
                     /translation="MKALERFGFDEEQLKRFAARAGEASDNFITATITPPTADDVSTL
                     PARGTPAHVELVKRGEDAIRAKQVGVIVLAGGMATRFGGVVKAAVPVIDNRTFLDLKH
                     ADVRQLSAKLGVPITTYVMTSYATHDRIVQQVRDEKLETDTTKIVCFPQMVSLRLLPD
                     GKVFLEDGEPSPYATGHGDLTFALRQSGVLGRFRRGGGTLLYMSNVDNLGATLDPAVI
                     GAHLARGAKITAEVVRKDAGDKGGAPARVDRVPQIVEAFRFPPSFDQNAIPLFNTNSF
                     VFDAEAIDRDFDLTFFRVEKKVDNTKVIQFERLVGQLTAFLPSHFLDVPRSGPDGRFQ
                     PVKDPEEMAARRADIEQILRARGVI"
     gene            complement(7422..8171)
                     /locus_tag="VFV99_20830"
     CDS             complement(7422..8171)
                     /locus_tag="VFV99_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061831.1"
                     /translation="MRFVVLSLAVHALVLWQFVRGHDDSLHVVRAEPTITTAEETPMV
                     VEMIGTVGGGDGGGGGGGGAATSAKPMTRAAHIGSTRARSVDPWAGLSVRTEDNREGN
                     RNGNGIGNRNGNGIGDGPGNGNGNGIGNGNGIGNGSGNGIGFGNGGGVHVERDVPPPP
                     PPPPPSKARPARLIWPTRDLEVEDESALFVARVTVDTDGAVVGARMVTNRPGSRAEHA
                     ANAIWTFRYSPALDEHGVPVRSTFEQSFQVR"
     gene            8250..9506
                     /locus_tag="VFV99_20835"
     CDS             8250..9506
                     /locus_tag="VFV99_20835"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5061832.1"
                     /translation="MRLAIVLSCLTVVSIAHADDIAERAKKLQREAIIVDTHLDAPDQ
                     LAGKWADVATRGATDHFDIPRAKEGGLTAPFFSIYVSAAYADKGAAKKALELIDLTKR
                     VVADHPNDMMLATTVDDARAAKKAGKLAVFMGIEGGHAIEDSLGVLREMYRAGVRYMT
                     LTHTNTNHWADSSGPFFLPDFDPKQSKVHGGLSDFGREVVKEMNRIGMIVDISHVSDD
                     TIDDVLEVSRSPVMASHSSCRALSNMPRNLTDDQIKRIAAKGGIVMINIGSGFIEQAS
                     WDAFVAGKKKLAPQLAALKKKYAKNPALYFKAMFEAYVKLGLPRATLAKVADHIEHVI
                     KVGGIEAVGLGTDFDGIDDVPTGLEDYSKLPALTEELLRRGHSDADVKKILGENFLAF
                     FSRVEAAKKKLAAEPVNPAVYKDTAK"
     gene            9637..10116
                     /locus_tag="VFV99_20840"
     CDS             9637..10116
                     /locus_tag="VFV99_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061833.1"
                     /translation="MRSLLGLLLLTGCMTAAESHGTTAKEYKGTVDVVVVNATPEKMC
                     GLYMSYDTDADYGDNWLPVAGLAVGQSAEFKVKPGTYKAKWNSCKDMADAPTANYSAT
                     LVAGTAFPLTEDIQLYAFVSDGTPPTKRAMPRPKLKMVKFIGQTTYIAKPTVTAAKE"
     gene            10186..13320
                     /locus_tag="VFV99_20845"
     CDS             10186..13320
                     /locus_tag="VFV99_20845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5061834.1"
                     /translation="MWLIRTALKKPYTFVVMAVLIAIGGGLSIRKAPTDIFPQIDIPV
                     ITVIWNYGGLPPAEMERRVANNFERFVTTIVADIEHVESQTLTGSTIIKIYLQPGADV
                     AQAIAQTSAVAQTAVRSMPPGAVPPLIMRYSATSVPIMMLALETDSLSEQQLFDYGIN
                     FIRSEIATIRGAQIPYPYGGKQRQIMVDIDPARLHALSMSPRDVQTAIGQQNVTLPTG
                     TAKIGTTEYQVIVDASPETLDELAGIPIKSVDGRTIYLRDVANVRDGGIPQTSMVHVE
                     GHRSVLMAIIKNGDASTLEVNARVKEALARAIDRLPKEARGHLTVKVLFDQSVFVRAS
                     IDGLLFEAAVAAGLTALMILMFLGSWRSTVTVIISIPLSILFSLIVLGLLGQTVNVMT
                     LGGLALAVGVLVDDATVAIENIHRNIGQRKPFEQAIIDGAAQVAMPALVATLCICIVF
                     APLAMLSGAAKSLFLPLAMAIVFAMLMSYLLSRTLVPTMVNYLERRAAMAHHRPNRFV
                     VAFERAFTSFRTMYGRWLAWALQHRAFAVSAFVLFVGASLMLAPQVGRDFFPSVDAGL
                     IKLHVRGAPGTRIEESEKRIAAIERTIRGVIPPDEIETMLDVLGTPYSSINLSLSEGA
                     LISPADAQIFIGLREGHRPTADYLRDLRQVLRQRYPDMTFFFLAPDISTQVLNFGLAA
                     PIDLQVIGPIGSEDQTLAFAEKLAARIDKVPGAVDVHLAQVSKSPQLIVDIDRNIAQR
                     AGLTERDVASDLLVSLASSGQVAPTYWIDKRGVQYVVSVQTPQYKNDSLDALKATPIS
                     TPGGEPQTLGNVAKINRGYGPQNITHYNVARTFDVQANVQDSDLGSVIDGIKPIVDDM
                     RKHAPPGTRIVLKGQAESMDSSFDGLRNGLVFAIVLVYLLMVVFFQSWLDPFIILMAL
                     PGALAGIVWILFLTGTTLSVPALVGSIMCVGVATANSILVVSFANEQRAGGRDGRAAA
                     LAAGMTRLRPVLMTALAMIFGMIPMATGLGDGGEQNAPLGRAVIGGLALATFATLFFV
                     PVMYSLLRRNVARN"
     gene            13304..>13753
                     /locus_tag="VFV99_20850"
     CDS             13304..>13753
                     /locus_tag="VFV99_20850"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5061835.1"
                     /translation="MSQETEELGFDLPQPAQASRARVIVILVLIVGGAGAFGYLKSHK
                     ARGQSTVVAKEQGPVRVEVMKAKVLSSDQALSLPGEVRPLEEAKIYSRSAGYVRKWYA
                     DIGDKVKEGQLLAEIDTPELDAQLAQARAQLASARAAVKQAAAQRDFA"
BASE COUNT         2345 a         4510 c         4452 g         2446 t
ORIGIN      
        1 ggcgacacca aggtccacat gaaggacggc ttgttccaga agggacacga ggcgagcttc
       61 acgacatcgc tgaccgtgcc cgaggacatc gcgcggaaga tcttcgtgca cggcgatgct
      121 gctgccggtg tccaggcatt cctcgagggc atcatcaccg tcgagggtga cctcgcgcag
      181 gtcgtagcga tgcagaccgt cgatccgagc gcggagcaga tgaagctcgc gcgccagatc
      241 gagacggtca ccgcagcctg atcacggaac gaagatcact tcggggttcg tctgcaccgt
      301 cgagacgcag ccgtcgcaga gctgaccgct gtcccagtcg ccgccgcccg gcggggtcga
      361 gacctcgagc tgacacgtcg ccgagcagcc gtcgccaccg gtcgtgttgc cgtcgtcgca
      421 ctgctcgccg ctgtcgatgt gaccgtcgcc gcagcacggc tgcggaggcg gcggcgtttc
      481 ggtcgtacac gtcgccgagc aaccatcgcc gttcgtcgta ttgccgtcgt cgcattgctc
      541 accggcgtcg agctggccgt tgccacagca cgacggcggt ggcggcggtg gcgtctcgct
      601 ctcgaggacc ttgaagttgt cggtcttcgt agaaccgggc acgaagccgt ggaacctgcc
      661 gctgctcgcg tatgcagcga caggcgtgat ccagaccttg tattcgccac ccgggttcgg
      721 cgtgtcggcg tacggcatca gctgaaccgt gagtgcgccg agcgacgcgt agtctcgatc
      781 gcttccgctc acgtgcgtgc atgcaccgac gaccgcgctg atcacgccgg acgtatcgac
      841 gtgaaagcgc cggcacgcga tgtcgtcggt cgatagcagt gtcttgccac tcggatcggt
      901 gacctggaag tagtagtcgc ctgcggcgag cgcagccgca tgcaccggcg aaccacccga
      961 gggaccacca tcgagataga cgtcggtctt ggcgtcgtag atgttgtggt tgaccgccga
     1021 gccgtcgggc aacgtcgtga agatcgcgcc gcgcacgcgg gcgccttgat cgacagacga
     1081 cagctgttcg tctgtcgctt caccgacgaa gcagctcgtg agcgtgagaa gcgagaatgt
     1141 gaggtacgtg ccgggtcgca tcgcttggtg ctcctacggc ccaacgggac cgtgcacggt
     1201 catcgagcaa acaaggtgcc gccagttacg aaatcctgtt gcgtcttcgg cgcgcacagc
     1261 gtcgacgagc gctcgcaggc atacgcgggc aacgtgcaaa ggcgcacggc caacaggtcg
     1321 accgctgtac gcaacagtga gcgccttgcc ccacgcgacg cgcgcgtgta gttgtggcgc
     1381 gcggtcggcg tttccgcgta ccgtcgatct gatgcggagt gtcgtcgctg tcgtttgcct
     1441 cgtcggcgct ggatgcgacg tcgtgtggcg gctcgacgac gtcagcggcg gcatttccga
     1501 tgcgccccgc gcggtagatg cgccgagcga tggcgacgat ccatgtccat gggggccgcg
     1561 cgttcagatc tcggccggcg gcgggaacca tgacccacag ctgagcgagg acgggctcga
     1621 gctgttcttc gtgcgcggtg cctcgggaac gtacgatatc tggcgcgctg tccgcacgga
     1681 tgtctcgcaa ccattcccga tgggcgtaga ggttgccgag ctgagctcgt ccgtggaaga
     1741 cacggatccg acgcttgcgg ccggtggtaa caccatcgtc ttcaagtcca cgcgtggcgg
     1801 caccggcaat cacgcgtggt tagcaacgcg tagcgggcgc ggtcagccat tcacgatacc
     1861 ggtcatgctc accggggtgc cggctacggt tgccggtgtc gatctctcgg ccgacgggct
     1921 gacgctctac gtcgatgacg gcggcaagtt ggtgtcggtg acgcgtatcg cgggaagcac
     1981 gacgttcatg aacgagaaaa cactcacgct cgagcgaacc cagtatccgt cggtctcggc
     2041 cgatggcctc gagctctatt acaacggtgc cggtgtcgtt cgccgcaagc ggcaaaccac
     2101 gaacgacatg ttcgatcccg ccagtctcgt ccagatcgat gacggcgccg ccgatcccga
     2161 tctcacgttc ggtgggacac gtctcgtgat gaccggcgcc ggtatcgtct acgtccgcga
     2221 acgctgcgcg ccgtagccac gaccgcacca cttcatccta atccgcgcta tagcacctgg
     2281 gaggaggaag cgatgaagaa ggcattgttg gcgatggttg tggtagctgc cgctgggtac
     2341 ggtgtgtacc gctggcgcgc ccacgaggcg cccgtcgcag cgaagaccga cagcaacctc
     2401 gtgacgaatc ggttgtggat cgatcacctg ccgcgcggcg agcgcgacac gatccaggtg
     2461 ttcgctgcga tcacggagga gccgatcggc gtgttcgagg ccgcatcgcg ctggaagggc
     2521 gagttcgagt tcttccgcta cgagcagcac ggcgacgagc tgcgcatctt cttcccgcag
     2581 tcgaacacgc gcgacaaggt ccgcgcgaag gccaccgagt gcaaccgcgg cggcatggac
     2641 tactgcctcg agctcgaagg ctcgagccac ggcgtcaaga agtactactc gcgcgaaggc
     2701 tgggaaatcg gcagcacgcg cgacgccgaa gtgctcatca acaagctcgc gctctgatcc
     2761 gcgcggtagg cgcaagtgcc acgcacgggc agttgccgca gacggctggt tgcactcttg
     2821 caactaatcc gctcatgggg cttctgagac gctcgcaagg catcaacgtc gcacgcaaag
     2881 atcgcaagta tgtcggccgc gcgggagcgg cacaggacat cgaggttgtc gccgcgagcg
     2941 gcagcgaatt gcgcgacgcg cagggccgca cgttcgtcga ttttcagatg ggctggtgcg
     3001 tgggcaatct cgggtggaat ccgccagaga tcctcgcgca cgtgcgcgcg ttcgaaggcc
     3061 cgacctacgt cgcgccaggc gtgctctacg agccgtgggc agagctcgcc gagatgctcg
     3121 tcgaggcgtc gcccgccaag ctaacccacg cgtttcgcac cgtcggtggt accgaagccg
     3181 tcgagctcgc gatgcagctc gcgatgcggc gcaccggccg tcacaaatgc gtcgggctcg
     3241 cgggcgcgta tcacggcaac tcgatcgcga cgcgctcgct cggatcttcc gaaggcgatg
     3301 cgcacctcgc gggcttcaag cagctcgagc cgcctctcga cgaagcagca ctcgatcgcc
     3361 tcgagacgat cctcaagcac gaggacatcg ccgcattcgt catggaaccg atcgtgctca
     3421 acctcggcgt cctgattccc gacaccgcgt tcatgaacgg gctcgacgag ctgtgccatc
     3481 gctacggcac gctcgtgatc atggacgaga tcggcacggg catggggcgc tgcggccgac
     3541 tgttcgcgag cgagctgttc gagctcgatc ccgacatcct cacgctcggc aaagcgatca
     3601 cgagtggcgt cgctgcgcta tctgccgtgc tcgcgaccga ctacgtcgcc gatgtggagc
     3661 tcgacttctt ttcgacgtat ggctggcatc cggtcgccgt cgatgccgcg atagcgacgc
     3721 agcagtactg gcgtgaacat cgcgacgtca tgctcgcaaa catcgccgag cgcagcgcgc
     3781 acctgcgcca gcaactgtcg ctgatggaat ttccgcgcga accggagcta cgaattcagg
     3841 ggctcgccgc ggccgtcgat ctcggcgatg ccgagtacgt cgcaaagctc gaggagcgct
     3901 gccgcgagga aggcctgttg atgtttgccg aggacgaggc gctcgtgatg ttcccggcgc
     3961 tgacgatcac ccagaacgag atgcaggaag gcctcgatat cctcgccgag tgtgtgaggc
     4021 gcgggcgcta gcagtcctcg acgccgatcc agagcggaaa gtccgcttcg cgtgcacgac
     4081 gccgcgcagt cgcgaccttg tcgtgcgccg cggcgagctt gtaacgtccg acgacggtgc
     4141 ggccctggcg atgcgtctcg agcatcttcg tcgtcgcgtc ttcatcgttg tgatcgaaga
     4201 cgtcacgcag cagtgccacg acgaactcct gcgtcgtgtg gtcgtcgttg cgcagcacga
     4261 tgtgaacatc ggtgcgatct ggaagctcgg tcgatggctc gcgcatcccg tggacgagca
     4321 cgaacaacag ggcgacggca tcgaacccag cggcagcggc ggcggccttc gcgctcggcg
     4381 tgcgtgcgag ccgggcccag acgtcggtga tcgtcgtcgc ccggccctgt gcgcgtgatg
     4441 cggcgaatgc atagccgacg acgcgcgcgc cttcctgctg cgcttcctcg tcgtgggagc
     4501 tcttgcgctt gtcgagctcg tcttgcacgt ggttctcgaa cttcgtcgcg tcgccgtcga
     4561 gcttgccgat cgcggcgacg aaattctcgt cctgcgcgag gccgtgcatc agatggagcg
     4621 accacagata cgcgtgaccg cgcgagcttg cctcgtgctg cgcgacgctc atcacgactt
     4681 cgagatcggg gtcgcgtggg atgtcgagga agaacttgcg ctcgcggcgt tgctgtagct
     4741 tccaccagcc gtagctggcg ccagcactcg ctgcgaggag accgaggatc tcgagaccca
     4801 cctcgctagc ttagctcagt cgcggcggat gcagaggtaa acgcgctcgg cgctgcggcc
     4861 gcgcaccagc tcgatcttcg tcgggtcttc gcccgcgcgg cgcttgaacg tgtgctcgat
     4921 cgcaggcgcg ccttggcctg accggaacgc ctgaacgtgc tccgccagcg agcggttcgt
     4981 cggcccgggc gcgacatctc cgaagaaatc tcggccgatg gcgcgccagc gctggatgcc
     5041 gctcttctcc gattccgttc gattcatccg cacgacggtc atcgactcgt tcaggcaaac
     5101 gacgccgaac ggcaggtcgt cgagcgcact atcgtcgaga tcatcgagca tgtggattgg
     5161 agtcgcgaaa cgagtagaac gttacactcg cttaccatga gtagggactc ggccggcaag
     5221 caagatgatg gttcagcgcc gctcgtcgcg atcgcggagc gatggctcgc gtgtttcgac
     5281 cgtcgcgatc tcgatggctt gctcgcgctg tacgccgacg acgccacgca tacctccccg
     5341 aagatccggg tccggcatcc ggagaccggc gggctcttgc acggcaaagc ggcgatgcgc
     5401 gcgtggtggc aagactcgtt cgatcggctg ccgagcatgc gttacgtgcc gaccgcgctg
     5461 accgcagatc gcgagcgcgt gttcatggag tatgtacgcc acgtcgatgg cgaacccgac
     5521 atgccggtgg cggaggttct cgaggttgtg aacggccgca tcgtcgcgag tcgcgtgttc
     5581 catggctgag cgcctacacg attggctcgc gcaacgaccg ttcgcgctcg cgatgtcgag
     5641 tggcttcttc tcgttcttcg cgcacacggg catgctgtcg tcgctgacgc gaaagggctt
     5701 cgcgccaaac ctcgttgccg gcgcgagtgc gggcgcgctc gtcggtggtg cgtgggcggc
     5761 gggcctcgac gcgcagtcgc tgcaggagcg gctcgtcgcg ctgcgtcgcg cggacttctg
     5821 ggatcccgca ccgggcgcag gcctgttggc cggcaagaag ttcgatcgca ttttgcgcga
     5881 cctgctgccg gtcaccgcga tgactgcgtg ccgcgtgccc gtgaaggtca gtgcattcga
     5941 catcgcgagc cgccgcacca ccgtgctgca cgacaccgat ctcgcgcacg cgatccgtgc
     6001 atcgtgcgcg gtgcccgcgt tgttccatcc ggtgtggatc gatcgccgcg cgtactggga
     6061 cggcggcatc ctcgaccgcc ccggcctcgc cggcattccc gccggtgaac gcgtgctgtt
     6121 ccatcacatc gcgtccaaat cgccgtggcg cacgcggctg cccgtgcccc gtcgcgcagg
     6181 catggtcacg ctcgtgatcg atggtctgcc gcgctcgggc ccgttcaagc tcgacgccgg
     6241 ccgccgtgcc ctcgagctcg ctcgcgacgc gacgctgcgc gcactcgatg ccccgatcgt
     6301 cgatgacgtc gtgcgcgtgg ctgcctagat cacgccgcgc gcgcgaagga tctgctcgat
     6361 gtcggcgcgg cgcgctgcca tctcctctgg atccttcacc ggctggaacc gtccgtcggg
     6421 tccgctgcgc ggcacgtcga gaaagtggct cggcaagaat gcggtgagct ggccgacgag
     6481 ccgctcgaac tggatcacct tcgtgttgtc gaccttcttc tcgacgcgga agaacgtcag
     6541 gtcgaagtca cgatcgatcg cctcggcatc gaacacgaac gagttcgtgt tgaacagcgg
     6601 gattgcattc tggtcgaagc tcggcgggaa ccggaacgcc tcgacgatct gcggcacacg
     6661 gtcgacgcgc gctggcgcac cgcccttgtc acctgcgtcc ttgcgcacga cctcggccgt
     6721 gatcttggcg ccacgcgcga gatgcgcgcc gatgacagcg ggatcgagtg tcgcgccgag
     6781 gttgtcgacg ttcgacatgt agagcagcgt gccgccaccg cggcgaaacc ggccgagcac
     6841 gccgctctgg cgcagcgcga acgtcaggtc gccgtgaccg gtggcgtacg gcgatggctc
     6901 gccgtcctcg agaaagacct tgccatcggg caacagccgc agcgagacca tctgtggaaa
     6961 gcacacgatc ttcgtcgtgt cggtctcgag cttctcgtcg cgcacttgct ggacgatccg
     7021 atcgtgcgtc gcgtagctcg tcatcacgta ggtcgtgatc ggcacgccga gcttcgcgct
     7081 cagctggcga acgtccgcat gcttgagatc gaggaacgtg cggttgtcga tcaccggcac
     7141 tgccgccttc acgacgccgc cgaagcgcgt cgccatgccg ccggcgagca cgatgacacc
     7201 gacttgcttt gcgcgaattg cgtcctcgcc gcgcttcacg agctcgacgt gcgcaggcgt
     7261 tccgcgtgct ggcaacgtgc tcacgtcgtc ggcggtcggc ggcgtgatcg tagccgtgat
     7321 gaagttgtcg ctcgcctcac ccgcgcgcgc agcgaagcgc ttcagctgtt cctcgtcgaa
     7381 cccgaacctc tcgagtgcct tcacctccgc agtctagcgt gtcagcggac ctggaacgat
     7441 tgttcgaacg tcgagcgcac gggcacaccg tgctcgtcga gcgcaggcga gtaccgaaag
     7501 gtccatattg cattcgcggc gtgctcggcg cgtgacccgg gccggttcgt gaccatgcgc
     7561 gcaccgacga ccgcgccatc ggtgtcgacg gtgaccctcg cgacgaacag cgccgattcg
     7621 tcctcgacct cgagatcgcg cgtcggccag atcagccttg cgggtcgcgc tttcgacggc
     7681 ggtggaggag gcggtggtgg cgggacgtcg cgctcgacgt ggacgccgcc gccattgccg
     7741 aatccgatcc cgttcccgct tccgttcccg atcccgtttc cgttcccgat cccgtttccg
     7801 ttcccgttcc cgggcccgtc tccgattccg ttcccgttcc tgtttccgat tccgttcccg
     7861 ttcctgtttc cctctctgtt gtcctcggtc cgtacggaca agcccgccca tgggtccacc
     7921 gacctcgcgc gcgtcgatcc gatgtgcgct gcgcgtgtca tcggtttggc gctcgtcgct
     7981 gctccaccgc cgccgccgcc gccgccatcg ccgccgccca ctgttccgat catctcgacg
     8041 accatcggcg tttcctcagc ggtcgtgatc gtcggctcgg cgcggacgac gtgcagcgag
     8101 tcgtcgtgcc cacgcacgaa ctgccacagc accaacgcgt gcaccgcgag cgacaggacc
     8161 acgaaccgca caggatgatt gtgcctcgcc gcgcggtcgt ctgcgatcac gtctgttgac
     8221 ggctgcgaac cggagtaagc tgccgcgaca tgcggctcgc tatcgttctc tcgtgcctca
     8281 cggttgtctc catcgcgcac gccgacgaca tcgccgaacg cgccaagaag ctgcaacgcg
     8341 aggcgatcat cgtcgatacg cacctcgacg cacccgacca gctcgcgggg aagtgggccg
     8401 atgtcgcaac tcgcggcgcg accgatcact tcgatatccc gcgggcgaag gagggcgggc
     8461 tcaccgcgcc gttcttctcg atctacgtgt cggccgcgta cgcggacaag ggcgccgcga
     8521 agaaggcgct cgagctgatc gatttgacga agcgtgtcgt cgccgatcat ccgaacgaca
     8581 tgatgctcgc gacgaccgtc gacgatgcgc gcgccgcgaa gaaggcgggc aagctcgccg
     8641 tgttcatggg catcgaaggc ggccacgcga tcgaagactc gctcggcgtg ttgcgagaga
     8701 tgtatcgcgc gggcgttcgt tacatgacgc tgacgcacac gaacacgaac cactgggcgg
     8761 attcgtcagg gccgttcttc ctcccggact tcgatcccaa acagtcgaag gtgcacggtg
     8821 ggctctctga cttcggccgc gaggtcgtca aggagatgaa ccgcatcggc atgatcgtcg
     8881 acatctctca cgttagcgac gacacgatcg atgacgtgct cgaggtatcg cgctcgccgg
     8941 tgatggcgtc gcacagctcg tgtcgtgcgc tctcgaacat gccgcgcaac ttgaccgatg
     9001 atcagatcaa gcgcatcgcc gcgaagggcg gcatcgtgat gatcaacatc ggctcgggtt
     9061 tcatcgagca agcgagctgg gacgcgtttg tcgcgggtaa gaagaagctc gcgccccaac
     9121 tcgcggcact gaagaagaag tacgcgaaga acccagccct ctacttcaag gcgatgttcg
     9181 aggcgtacgt gaagctcggc cttccgcgcg cgacactcgc gaaggtcgct gatcatatcg
     9241 agcacgtgat caaggtcggc gggatcgaag ccgttggact cggcactgat ttcgacggca
     9301 tcgacgacgt gccgaccggg ctcgaggact attcgaaact tcctgcgctc actgaagagc
     9361 tcttgcggcg cggccacagc gatgcggacg tgaagaagat cctcggcgag aacttcctcg
     9421 cgttcttcag ccgcgtcgaa gcagcgaaga agaagctcgc cgccgagccg gtgaacccag
     9481 cggtctacaa agacaccgct aagtagcatc gctacttcag ttagctgtgt acgacatatc
     9541 ctcgacgagg aagcgcgctt accaatggac gtcgtcggta ggttagttgt gagtttggcc
     9601 ccagctgcac acatctgccc ggcgtagcgt ggggccatgc gttcactgct tggtctcttg
     9661 ttactgacgg gttgtatgac ggcggccgag tcgcacggca cgaccgcgaa ggaatacaag
     9721 ggcacggtcg acgtggtggt cgtgaatgcc acgcccgaga agatgtgcgg cctctacatg
     9781 agctacgaca ccgatgccga ctatggcgac aactggctgc ccgtggcggg cctggcggtc
     9841 ggccagtcgg ccgagttcaa ggtcaagccc ggcacgtata aagcgaagtg gaatagctgc
     9901 aaggacatgg cggacgcgcc gactgcgaat tactcggcca cgctggtcgc cggaaccgca
     9961 ttcccgctga ccgaggacat ccagctctac gcgttcgttt cggacggcac gccgcctacg
    10021 aagcgcgcga tgccccggcc gaaactgaag atggtcaaat tcatcggcca gacgacttac
    10081 atagccaaac ccacggttac tgcggcgaaa gaatagaccg ttccgcgttg cgaaatgttg
    10141 agcggctaga cagccgctcc attgggggca cactggaagc cctcgatgtg gctgatccgt
    10201 accgcgctga agaagccata cacgttcgtg gtgatggcgg tcctcatcgc gatcggcggt
    10261 ggcctgtcga ttcgcaaagc gccgaccgac atctttccgc agatcgacat cccggtcatc
    10321 acggtgatct ggaactacgg cggtctgccc ccggcggaga tggagcggcg ggtcgccaac
    10381 aacttcgaac ggttcgtgac cacgatcgtc gcggacatcg agcacgtcga gagccagacg
    10441 ctgacgggca gcacgatcat caagatctat ctgcagcccg gcgccgacgt cgcgcaggca
    10501 atcgcgcaga catcagcggt cgcgcagacc gcggtgcgct cgatgccgcc cggcgcggtg
    10561 ccaccgctga tcatgcgcta cagcgcgacc agcgttccga tcatgatgct cgcgctcgag
    10621 accgactcgc tgtccgagca gcagctgttc gactacggca tcaacttcat ccgctccgag
    10681 atcgcgacga tccgaggcgc acagatcccg tatccgtacg gcggcaagca gcgccagatc
    10741 atggtcgata tcgatccggc gcgcctgcat gcgctcagca tgtcgccgcg cgacgtgcag
    10801 acggcgatcg gccagcagaa cgtgaccttg ccgaccggta ccgcgaagat cggcacgacc
    10861 gagtaccagg tgatcgtcga cgcgtcgccg gagacgctcg acgagctcgc cgggatcccg
    10921 atcaagtcgg tcgacggccg cacgatctac ctccgcgacg tcgcgaacgt gcgcgacggc
    10981 ggcatcccgc agacgagcat ggtccacgtc gagggtcacc gctcggtgct gatggcgatc
    11041 atcaagaacg gcgacgcgtc gacgctcgag gtcaacgcgc gggtgaagga agcgcttgcc
    11101 cgcgcgatcg atcggttgcc gaaagaagcg cgcggccacc tgaccgtgaa ggtgctgttc
    11161 gatcaatcgg tattcgtgcg cgcgtcgatc gacggcctgc tcttcgaagc cgcggtcgcg
    11221 gccggcctca ccgcgctgat gattctgatg ttccttggca gctggcgctc gacggtgacc
    11281 gtgatcatca gcatcccgct atcgatcttg ttctcgctga tcgtgctcgg cctgctcggt
    11341 cagaccgtca acgtgatgac gctcggtggc ctcgcgctcg cggtcggtgt gctcgtcgac
    11401 gacgcgaccg tcgcgatcga gaacatccac cgcaacatcg gccagcgcaa accgttcgag
    11461 caagcgatca tcgatggtgc cgcgcaggtc gcgatgcccg cgctcgtcgc caccctttgc
    11521 atctgcatcg tgttcgcgcc gctcgcgatg ctgagtggcg ccgcgaagtc gctgttcctg
    11581 ccgctcgcga tggccatcgt gttcgcgatg ttgatgtcgt atctgctgtc gcggacgctc
    11641 gtcccgacga tggtgaacta cctggagcgt cgcgcggcga tggcgcacca ccggccgaat
    11701 cgcttcgtcg tcgcgttcga gcgcgccttc acgagcttcc gcacgatgta cggccgctgg
    11761 ctcgcgtggg cgctgcagca ccgcgcgttc gcagtcagcg ccttcgtact gttcgtcggt
    11821 gcgtcgctca tgctggcgcc gcaagtcggc cgtgacttct tcccgagcgt cgatgccggc
    11881 ctgatcaagc tgcacgtgcg cggcgcaccc ggcacgcgca tcgaggagag cgagaagcgc
    11941 atcgccgcga tcgagcgcac gatccgaggc gtgattccgc ccgacgagat cgagacgatg
    12001 ctcgatgtcc tgggcacacc gtactcgagc atcaacctgt cgctgagcga aggcgcgttg
    12061 atctcgccgg ccgacgcgca gatcttcatc ggtctccgcg aggggcatcg gccgaccgcc
    12121 gactacctgc gcgacctgcg gcaggtgctg cggcagcgct atcccgacat gacgttcttc
    12181 ttcctcgcgc cggacatctc gacgcaggtg ctgaacttcg gcctcgccgc gccgatcgac
    12241 cttcaggtaa tcggtccgat cggcagcgaa gatcagacgc tcgcgtttgc cgagaagctc
    12301 gccgcgcgca tcgacaaggt gccaggcgcc gtcgacgttc acctcgcgca ggtctcgaag
    12361 tcgccgcagc tgatcgtcga tatcgatcgc aacattgcac agcgcgctgg cctcaccgag
    12421 cgcgacgtcg cgagcgatct gctcgtgtca ctggcatcga gcggccaggt cgcgccgacg
    12481 tactggatcg acaagcgcgg cgttcagtac gtcgtctcgg tgcagacgcc gcagtacaag
    12541 aacgactcgc tcgatgcgct caaagcgacg ccgatctcga cgcccggtgg cgagccgcag
    12601 acgctcggca acgtcgcgaa gataaatcgc ggctatggtc cgcagaacat cacccactac
    12661 aacgtcgcgc gcacgttcga cgtccaggcc aacgtgcaag acagcgatct cggctccgtg
    12721 atcgatggga tcaagccgat cgtcgacgac atgcgcaagc acgcgccacc cggcacgcgc
    12781 atcgtgctca agggccaggc cgagagcatg gacagctcgt tcgacggcct gcgcaacggc
    12841 ctcgtgttcg ccatcgtgct cgtctacctc ttgatggtcg tgttcttcca gtcgtggctc
    12901 gacccgttca tcatcttgat ggcgctcccc ggcgcgcttg ccggcatcgt ctggatcctg
    12961 ttcttgaccg gcacgacgct gtcggtgcct gcgctcgtcg gatcgatcat gtgcgtcggc
    13021 gtcgcaaccg ccaacagcat cctcgtcgtc tcgtttgcaa acgagcagcg cgctggcgga
    13081 cgcgacggcc gagcggccgc gctcgccgct ggcatgactc gcttgcgccc ggtgctgatg
    13141 accgcgctgg caatgatctt tggcatgatt ccgatggcga ccggcctcgg cgacggcggc
    13201 gagcagaacg cccccctcgg ccgcgcggtg atcggcggcc tcgctctcgc cacattcgcc
    13261 actttgtttt tcgtgcccgt gatgtacagc ttgttgaggc gcaatgtcgc aagaaactga
    13321 agagcttgga ttcgacctcc cgcagccggc gcaagcatcg cgcgcgcggg tgatcgtcat
    13381 cttggtgctc atcgtcggtg gcgctggagc attcggctat ttgaagtcgc acaaggcgcg
    13441 cggccagtcg accgtcgttg ccaaggagca agggcccgta cgcgtcgagg tgatgaaagc
    13501 gaaggtgcta tcgagcgatc aagcgctctc actgcccggc gaggttcgcc cgctcgaaga
    13561 ggcgaagatc tactcgcgca gtgccggcta cgtgcgcaag tggtacgccg acatcggcga
    13621 caaggtcaaa gaaggccagc tgctcgccga gatcgatacg cccgagctcg acgcacagct
    13681 cgcgcaggcg cgcgcacagc tcgcctccgc ccgcgccgcg gtcaagcaag ccgccgcgca
    13741 gcgcgacttc gca
//
LOCUS       DASUFX010000522        27762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_778227,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000522 DASUFX010000000
VERSION     DASUFX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27762
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778227"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFV99_20855"
     CDS             complement(<1..863)
                     /locus_tag="VFV99_20855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5061836.1"
                     /translation="MAQTRRSPRRPVRGAPIVRKNTVRLTGDVASRVRLGHPWVYREA
                     LGPRPIAPEPGTQIDLIDPDGEFVGRALYDADSAIALRVFVRKPDVAIDGRLIRERVR
                     AAIALRKRVVDLAKLGCVRLVNGESDGLPGFVVERYGDYLVAQLYSASVANLRDDFYG
                     ALEEELAPKAIYEQRRYRSLAGEAPRQAAAELVRGNPAPVEVEVIEDDLSFVVDVTAP
                     LSTGLFADLREGRRAVRQWAKDRRVLNLFSYTGAISVYSHAGGATEVCAIDVAAKAHA
                     RARRNFSASGF"
     gene            924..1214
                     /locus_tag="VFV99_20860"
     CDS             924..1214
                     /locus_tag="VFV99_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061837.1"
                     /translation="MNDPRPLATLEPVRFGEFLLHRNLITDDQWLCALAQHWSASSSG
                     RMPIGSTIVANGFLARDVVEREARMFHEDIEVVEVIPRSERNTMPLPNVPRA"
     gene            1280..2596
                     /locus_tag="VFV99_20865"
     CDS             1280..2596
                     /locus_tag="VFV99_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061838.1"
                     /translation="MMAVDSKQGGSSVPATGTPSAGTPSAAQTLAGMPAVAANGKNKR
                     KRPSSLLAQATSLPSVESSLDDFIARANETLGDANFDAARIAEKELKQEDEKRKEADA
                     LRWKATEQQMRESQAREESLRRQLDGLQGKLAEAEARAAVASSGGSQDGVIADLKYRL
                     NSLEESKRSAEDRSAQLAQELVLAKASQAAAPAAPFVSESFSNADTEELEQRARLAEA
                     KAAKALAAAKAAAAGLTVNPADLAAIESGLVVPMETTKKGTNWGLVFVALLVGGAIAA
                     AAVFVLNKKDTGSKPAAASVAPTEQAQPPAAAPAPAPSKPTVTPIEEPAAAQPAQPEA
                     VAAPAAAPEAKATPAPVETKAEPAKVEAKAEPAPAEPAKVEKKAAAPAKKAAAPTKKA
                     ATKTSKPSGGLADPFGGGETAPAPKKTKPAKKAAGGGGDTGIVDPF"
     gene            2849..4252
                     /locus_tag="VFV99_20870"
     CDS             2849..4252
                     /locus_tag="VFV99_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061839.1"
                     /translation="MKRSIILCIAIALGTTALTSSAWAEDPAPSKEQLEAAKKAFGEG
                     KALYDEQKFAEAVEKFKESYRLSRNPLLLYNIGHTLDQAGAKDKALFYYRKFLADAPP
                     NAAQRKDVQKRVEVIEKENLEADLNGTSVTTTPATATTPTGPKPEEKKVVQIKPAGTY
                     SEKDFQHQLVEAAPPGKPLDITAFVPEDSGFVVTLYYRGSGDAKFIAKPMKWRYKELV
                     GRVPANKVYGSSIQYYIEVKDQAGTAVAKSGKSTSPNLINIEQGATPRFYPDLTDDGE
                     QAMTAAQVKHHDEEDPILGKQGSKQEVDTEGDTHKDTTEEQQLTTTPPGGGNGFTDVG
                     SSKFTKAKWISTGAAGVFIGAAVATYIMAKKQASNLEADTMCGMTPPPCRAFDKDYDL
                     AVQDAGKRYNTWHKATTVLGIGAAGVAAYFWYKELTAKKHGELKVTNKNASPETTWAI
                     TPSITDGFTGAAAAASF"
     gene            4261..4869
                     /locus_tag="VFV99_20875"
     CDS             4261..4869
                     /locus_tag="VFV99_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061840.1"
                     /translation="MRSLFFFALVGLPIWATACTPYDPDLGMSPFLCGPADQNPRCPD
                     GYTCEMTSSGEYCLAMGGMVPVDASNVNCADDSALEPNDSIAQAFQTPVATTKNTLTF
                     AGLAICPMGDKDDYSVTITTANQNLEVIIEYDAVAGADLQGSILNSGGQAIANASPMG
                     TGMRRAYTPNLPTGVYYASVYGPTSGALQTNNYKLTITVTGP"
     gene            complement(4873..5880)
                     /locus_tag="VFV99_20880"
     CDS             complement(4873..5880)
                     /locus_tag="VFV99_20880"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5061841.1"
                     /translation="MRVTVGLLAVAFAASACSVDTSGNVKGQACRALTATSPATAALE
                     SPFQGTVFTIVIENKSRAQLLEGTAAPYLKSLAHQYTIANGYTDARVHPSEPNYIWMV
                     SGQNFGILDDGEPAEHHIASTSHLADQLEGVGRTWKTYQESMGEPCKIVNDGKYAPKH
                     NPFVFFDDVVGWNGKTPLRQQRCKDHVVDYSELEKDLANDTVPDYVFITPNLINDMHD
                     GTIAQGDAWLAREVPKILASKAWQNGGVLIITADEGEGRSATDWSQEDDPPFVVVSPL
                     AKKGYVSNTPYDTSSFLKTVQAIEGVEPLPCGTAASADAPTMDDLFTVPLPAMLPPAG
                     A"
     gene            complement(5933..6136)
                     /locus_tag="VFV99_20885"
     CDS             complement(5933..6136)
                     /locus_tag="VFV99_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061842.1"
                     /translation="MGDFLEDPMSYAEAKQIALLRLELRTCWESHDYAGAGVALALLS
                     QVAGNDNELTAEVKRWAIKLQVA"
     gene            6313..8007
                     /gene="pilB"
                     /locus_tag="VFV99_20890"
     CDS             6313..8007
                     /gene="pilB"
                     /locus_tag="VFV99_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX5061843.1"
                     /translation="MSRIGELLVREKMLSLQQLQQAQDEAKRTGKRLGATLARLGYVD
                     DTRLTQFVAKQYSLPSINLSEIEIDPAVLKLIPREICEKHQVIPIRRNGPTLVVAMAD
                     PSNIYAIDELKFLTQYNIEPIVTSESGLEAALSRYYDKGPDLDQMVGELDVEDVDFAS
                     MTDENVNVVDLENQAGEAPVVKLCNAILLSAIKKKASDIHVEPYEKSLRVRFRIDGIL
                     QEEMRPPLKLRNAITSRLKIMASLDIAERRLPQDGRIKLKIGGNKEMDFRVSVLPTLY
                     GEKIVMRLLDKSNLQLDMTKLGFDPKPLNDFKVAIKKPYGMVLVTGPTGSGKTTTLYS
                     ALSELNTIDTNISTAEDPVEFNLHGINQVQMHEDIGLNFATALRAFLRQDPNIIMVGE
                     IRDFETAEIGVKAALTGHLVLSTLHTNDAPSTVSRLLNMGIEPFLVTASVNLVLAQRL
                     ARRICADCKIPAERHVEALLKMGMTEEQARKGTIMKGKGCGTCSQTGYKGRVALYEVM
                     PFTDPLKELVLQGASAAEIKTEMIRGGTQSLRLAGIQKILEGVTTPEEVLRTTVDD"
     gene            8007..9131
                     /locus_tag="VFV99_20895"
     CDS             8007..9131
                     /locus_tag="VFV99_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5061844.1"
                     /translation="MAQLNLQALLKAMLDKGASDLHVTAGSPPRLRIDNELVRLQTDP
                     LGPVDTKTLCYSVMNDAQKLRFEEDLEIDFSFGIRGLARFRANVYMQQSCVAGAFRLV
                     PYQIIPLEELGLPGVVSDLCEKPRGLVLVTGPTGSGKSTTLASMIDRINTRMRGHIVT
                     VEDPIEFQHSHKACLVNQREIGRDSQSFKRALKYILRQDPDVVLIGEMRDLETIEAAL
                     AVAETGHLCFGTLHTNSAIQSINRIVDVFPSHQQEQVRAVLSFVLEGVVSQTLIPKSN
                     GVGRTLAAEVMIPNAAIRNLIREDKLHQIYSQMQIGQSKFGMQTMNQALCDLYIRKVI
                     SLEDALGHTSELDELKTMILNAGGSLGQHSGPATAPTRRS"
     gene            9143..10354
                     /locus_tag="VFV99_20900"
     CDS             9143..10354
                     /locus_tag="VFV99_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5061845.1"
                     /translation="MAKFNWEGTTRAGEKRRGVMEAETRAVVEERLRSDNINIQSVKR
                     EGGLGDIQIQIGTGVSAKDLQIFTRQLATMIDAGLPLVQCLDILAAQSENKSFKKILT
                     AVKSSVEQGATFSDALRKHPKVFDELYVNLVAAGELGGILDTILNRLATYIEKAVKLR
                     GQLKSAMYYPIGILVVAIGVIAVMLVKVIPTFEAMYHDMGNAELPAATRFVINLSHSF
                     LNKWYIFAGTLFGLVFGTIAARRTDGGREAIDRMLLRMPVIGSVLRKIVVARFTRTLG
                     TLLASGVPILDALDICARTSGNRVVQAGILRAKDKISEGHDMASPLAESKVFPTMVVQ
                     MIGVGEQTGAMDQMLQKIADFYEEEVDTAVAGLTSLIEPVMMAFLGVVVGGLIIAMYL
                     PIFKLAGNIQG"
     gene            10351..12048
                     /locus_tag="VFV99_20905"
     CDS             10351..12048
                     /locus_tag="VFV99_20905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5061846.1"
                     /translation="MTGATIGVRLTTPDASPGDSLVRAPALPDSDVRRRVTRLLLLRT
                     IVVSVVLGLSLLALVRGEAPARAAVWLQSAIIAATYLSSIVFGVLLRQGFSPKRVARP
                     MMANDLVLTSALVYVTGGAMSPYTFLYALTIVAAGALSYRRGAVIVTLASLLLVLVVS
                     LLAWHRTIDLPLSAQVRPWEQSTAELVRTLGINVAALIGVGALSFIFGDQLQKSADTL
                     ATTRRAAAELLTLHQDIVRSLSSGLITTTPDGQILTTNQAAIDILRTTAGGLIGRPID
                     SIMPGLTALLTKNKGELRRADLTVPTSDDNRTIGVTVSPLRDVRDEIVGCVINFQDLT
                     ELKRLEMHARRTERMATVGQLAAGVAHEIRNPMAAISGSIELLKQSSETSDDDRALMT
                     IVQREVQRLNVLIGDLLDYANPRPKQAVDFDLGSLVQETVQVARGDQNFSEIEITAAT
                     DEPLAIHADPAKLRQVVWNLVRNAADAAGGGGKHVKVEARRDGGGAKISVADDGPGIP
                     DAVLGRIFDPFVTTKQKGTGLGLATCHSIITEHGGRIDVETAPGKGTKMVVTIPPPR"
     gene            12058..12381
                     /locus_tag="VFV99_20910"
     CDS             12058..12381
                     /locus_tag="VFV99_20910"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX5061847.1"
                     /translation="MNCPICTQALEGGELLMVCTTCHKNLGSTSLSVGATGEFRVPTP
                     EMIAAVAAAEPGERPDATDLCSWCGKAEHQVKKLLGRSGTALCNECVSLACDIMDAEL
                     GTNWR"
     gene            complement(12378..13049)
                     /locus_tag="VFV99_20915"
     CDS             complement(12378..13049)
                     /locus_tag="VFV99_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061848.1"
                     /translation="MRFVARILGVAIVVLLLPFVGGNTLGRPGTFAGIGLGLVAVFWL
                     WLWLPRSAHDAFETGKYTAAQRRYRLLGMLAPSAIRERAALLSRAGCLIASDQLAAAE
                     RLLASIDAAQLAAAERAVWLNNRACAALAAGRDPKDILVLVDEASALRPDVPALQHTR
                     GMALLAVGRVDDAIAVLDGMRAGGELPARLEAERCRDLAKAWSQKGEAAYAEDYRLRA
                     EALAR"
     gene            13109..14380
                     /locus_tag="VFV99_20920"
     CDS             13109..14380
                     /locus_tag="VFV99_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061849.1"
                     /translation="MRTVPVLLISVITHGALLALVADWLTPVPTQRLAWRPVTEPPPP
                     PVHLPRATAEPIEIAFLADEPVTVAAADPVHERAPAGAAAVRTPGSAAITMTATSGGT
                     SESGREPAPGGAGPATSGFMHMRGPDLGLSAGTAERIASEPQKPRDELHKSGKLESQP
                     GGRAVIYDRVTTVSVDADGTAHFDDKKDIDLHFKLPIPKIWEIEEMRKDLGHELTEWF
                     KDPEAGKRYGRMQDVARHLTAVEGACDSWGDLMCDDPLAPQIEKRVRERKPKGGRGFI
                     GTFLAGPMDITAWLHRKYVGDPYASRKLKLLDDTRDERVAMGIAHRQQQSVRSAEYMT
                     RNLQALWAREQSPVARRAALFEMWDECDEDDAGLRARAVVIGWIRSHLPAGSADAYTE
                     AELAALQSHKGSSAEFAPYADVDLSITKRGE"
     gene            complement(14396..17644)
                     /locus_tag="VFV99_20925"
     CDS             complement(14396..17644)
                     /locus_tag="VFV99_20925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5061850.1"
                     /translation="MVLLFAVRVVYAQPATVGSVRGQLVDRTSGGPAAGAIVIATSES
                     LQGEQTAITDQRGAYFLTALPPGVYRLTVYYLSHQFSRREVLVQLGKQVVVNFALDTG
                     AEPGEVIAIQGQAPVVDQSSTKLGVVLTPEYTRNIPTGRLFGDTVGAVAGAQNDRYGV
                     SIAGTTSPETAYYVDGIAATDPLLGALSTQLPTEFISQTEVITGGYDAEFGRSTGGVV
                     NVITKRGSDAFHGSVFAHYTPAALAAKAATIQREGSAIDTRTVRGDSYDVGGEVGGPL
                     VPKRLWFHAGFMPSVSHETRERIIAHQVDRDQDGVPDVDPTTEYTLHEHVATTSLPLH
                     STTYYFTAKLTGAVNEHHQGEVVAFGNPSTATEIPFGIVRAPATLVWDHDFGSYNLAA
                     KWTSKLAAATQLDATAGFHRTYDLPHARDERPQVRYAYDRSLADFADVETENLDACRD
                     SIVDDGTDLYPKIVNCPVFGYAEQGLGVVAPRRDDRATLALSVTHRAKLAGYHTIKLG
                     SELERARDRTSYHFTGGYALARASADSRAGAPGRWQLTETMRVVRNLTAEERANPSSI
                     MLGAGQEVCPDRDAVCALADPVHTSTSQVDIGTYLQDSWLITPSFTLNAGVRWDHQAG
                     YVADDVVGTVTPEGETVGARVFALDNFAPRVGAIHDPTGEGRAKLFGHYGRFYETLPL
                     TGSDLFANALFTRTTLNVNRLQANTPRYDPNCTVDHDTPNLTDTIRACTDRSARAGLQ
                     AFEFVSPGLRGQYTDEIVLGAEYEVRPDLKVAAGFQHRIVPVVIEDVSMDGGNTLFIT
                     NPGESFDAEAAELHARAAELAGSNELRDRQLAETYETRAQWLDDVDQLDKPVRTYNAL
                     QLVATLRPTPRALLLASYTYSANRGNYPGLFSPETGQLLPNLNAFFDLPSVLPNRTGI
                     LGLDRPHKLHADGFYRFDLAPATALTVGASVRVQSGIAYSALATHPTYGGGESYLLAA
                     GSMGRTPVTGQLDVHLAFARGLLKSTSLEAFVDVFNLFDAQSETAVDQRYTLDTANQV
                     VGGIVEDLEHVKAIDPSDFTEQNRVIKKNPNFAHTSGRQDPRAIQLGLRWLF"
     gene            complement(17659..20049)
                     /locus_tag="VFV99_20930"
     CDS             complement(17659..20049)
                     /locus_tag="VFV99_20930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061851.1"
                     /translation="MGAPGGDETLPSHASTSDAATERFAIGSLAAERYRIERFIAAGG
                     MGEVYAAHDLVLDTKVALKTLRPELEGSADAIARLRREIALARSVTDPHICRLHDVGE
                     HDGRVFLSMELLEGRTLAELVKAGGMALDEIERIAPQLVAGLGVLHRASIVHRDFKTS
                     NVIVVGDRAVITDFGLARSVDAKDSRLTVESGLLGTPAYMAPEQVEARAVTPASDIYS
                     LGVVLFELLTGKLPFDEDTAMATATARLTKDPPRPSSLRSDIPARWDAIVLRCLARDP
                     AARFASVDELLGHKPVSRRWFLAVGGGGIAAAALGVWKFAASSGANTPAVTRSPDDLI
                     AVLPVLGAGPWIKDSWRTAITFDLHSALVQLGMPVLALHTGAMDATLNGAASRLLDEK
                     DPVAAALAVPHVVTAVTMTIARTATHVELAVTARGRESWSQTLRRPLTEVALVVHDAA
                     TAIAGRLGYAPPIAPVDARHIAADGYTHYGDTVGRMYRQPTPEEIEAFTQKRMATPFT
                     QLADLVEAQPRLARAQDIAADRLVMAAEQRNDYTKVEKTLTIVDKLTDEAIAADPRDA
                     LMHAVRGHAAMIRWDWKRADEETKRGFELAPLHERVAYHRSNFLHLVGRFDEALPIFE
                     TQHRINPNPRLGDGPLAWAYFYARRYPDAIRVLEPVMNRFDLTNFVEATYATTLPLAY
                     VELARFDDAFRVAETLRPYVDDDNLAVLLPVYLAAGKTDVVKAIREKLGDNIDFMVGA
                     ALAEANGDLEGSLTLLEQMVASHNIGALFLKVERYSPQMRSHPRFQALLKQVGFPS"
     gene            complement(20068..20532)
                     /locus_tag="VFV99_20935"
     CDS             complement(20068..20532)
                     /locus_tag="VFV99_20935"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5061852.1"
                     /translation="MKNIIAVSILVSACATTGRSLPVESSGTGIGPTFNGQLAQSTDE
                     AKPWFPMLASDAVLPSVAHRQVELSTDRDRYTFAVRLCVAPDGKVSKVDITRPSESSE
                     LDRAAMHDIAQWQFEAFSAPQTVRVCKQLDLAYEPQSEKSHIAIPLVRTSQP"
     gene            complement(20655..22205)
                     /locus_tag="VFV99_20940"
     CDS             complement(20655..22205)
                     /locus_tag="VFV99_20940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061853.1"
                     /translation="MVDDASTLMPAAPRSAARAGDVITLTIAYHPDVSRVGERARLEG
                     NDLDISRSTPMFSAPGSREQRGLGDRGLSRNPIVKLVRSGEGVALELASTTPAQLDGR
                     PLAGAGARAWQKLDQAALERGAVLEIADRVVLVVHLAAPDGERAPRCGLVGDSDSLER
                     VRADISRVADLPVPVLVRGETGVGKELVAHALHASSPRASRQFLAINIAALPPTTAAS
                     ELFGHARGAFTGATHDHDGMFARADGGTLFLDEVGETPFEIQPSLLRVLETSEVTPLG
                     SARSRKVDVRLIAATDADLELQISESGFREALFHRLAGYQLLVPPLRDRRDDIGRLLV
                     HFLRDELVSTGDADRLARQVDADLWLPSSLVARLVRHMWPGNVRQLRNAARQLAIANR
                     GASEVRADSALERLLAATAPAPSSPVAAQARRDPSAITDDELIATLQSVGWSAAAAAA
                     RLGVSRSSLYLLIDRCPKIRKAKDLPDAEVRRVYDDCAGELDAAAQVLQVSRRALKLR
                     WAELALAK"
     gene            22332..22598
                     /locus_tag="VFV99_20945"
     CDS             22332..22598
                     /locus_tag="VFV99_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061854.1"
                     /translation="MRTSIDLNDRLLTLAKHAATKRGITLREVFEQALRAYLEPSRKQ
                     EGYKLELGITHGEVVPGVPVDDWSALRGYVAELDTADDFKRYRK"
     gene            22595..23098
                     /locus_tag="VFV99_20950"
     CDS             22595..23098
                     /locus_tag="VFV99_20950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEX5061855.1"
                     /translation="MIAIDTNIIVYARRAELPHHRAARTLLTQLAQGATPWAIPWPCL
                     YEYIKVVTNPRLFRPPDTLADALADVESLVASPSVVLLGHGPEHVRHLRRAADDGHPI
                     GGQIHDAHIVALALEHGVTELLSSDRDFRRFSTLNVRDPFGSSRAGEPRGRYRVRGRR
                     RAAHAQP"
     gene            23133..24512
                     /gene="miaB"
                     /locus_tag="VFV99_20955"
     CDS             23133..24512
                     /gene="miaB"
                     /locus_tag="VFV99_20955"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5061856.1"
                     /translation="MSERLVPLGKRTAEAPMRPQPAADAPKVFVETYGCQMNVADSDL
                     IGSVLADAGYAQATRADEADVIVVNTCAVREKAEERVIARASELGALKRKRPGTVLAI
                     VGCMAEHLKEGLAERAPAVDVIAGPDSYRRMPDLLAAARAEHAHPLVDIKLDKAETYE
                     GLSGAIGGDGVSGFVTIQRGCDKFCTFCVVPFTRGRERGASPREILRQVRELVAAGYR
                     EITLLGQTVNSYVWEDVTFAQLLRTVASVDGLERIRFTSPYPVDFTDDVVAAIAEESK
                     VCKYVHLPVQSGSDVVLERMRRGYTVADYRAIVARLRAAMPDIALSTDILTGFSGETD
                     DDHAQTLALMREIRFDSAFMFAYSERDLTFAAKKLPDDIPAAVKQKRLAEIVALQETI
                     SAEVFAAQVGKRERVLVTGPSKRDPAQLIGRTDGFKSVILPAGVGSVGGFVDVTIERA
                     TMATLFARA"
     gene            24522..24908
                     /locus_tag="VFV99_20960"
     CDS             24522..24908
                     /locus_tag="VFV99_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX5061857.1"
                     /translation="MGRTWVLVFCVACAHGAGAGGGGGGEASFAITYPDAPPTGAVAK
                     HGKRFYAKPEGRCLYENGREAHWTIGGARVTSGELPPGLTIEDGQIGGVPTQTGTYKF
                     HIEFSNVMCAGKPYTGQSVDGQITVQ"
     gene            24905..26725
                     /locus_tag="VFV99_20965"
     CDS             24905..26725
                     /locus_tag="VFV99_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061858.1"
                     /translation="MSHQHPLVELSLAALGRLAKVPRFGEPAHGEDAELEKIWVRAPL
                     SVAIGGAVSARTELFNYLCGKKVLDPDGRQGGCAGLRIRRGRSTRFKATRDDGTIEEH
                     LLPPEQADDDALRMRAEAAEAEVKERKLALQRVEHALPRAARARPRGLMIWLWPIWWL
                     LTRKHRRVLADRRFTELAYDQACDAREAANRELEEAEQRIRVQRGRFFESLRALSSGP
                     PLGSNVREVELVLGEGPLPEGVELIELTRSSQASELVDAIFLVEKDRLHAPHGDEGTA
                     LAVGKINDVIPTLPLMLGKARALVIARTARDVIEPALELLDDEVNDTEEGFRLRIERL
                     EAMQILDANEFTRTVLANIKPQIAQSIHMVIEHGAAHLGAELQRLGHEWQSGIAMTQT
                     NDELKVAVQRIEQSAPLDAKRIAEEVRLLVMGGAAGAAYDLYPELLGTLKPHGLEENP
                     PKAAPPLPALELLPSLTNASPAKLSGAAGWLGGLLRSFESRRADVMQKAQARMEHLRE
                     VANAEILDAEPKLFQAIDHALNAFVMTAISRQEAWLDGAMTLERETVAREGQELAPLA
                     RARDLLKRDIGKLLAGIAALEKENAGLAAAAAANPVISAA"
     gene            complement(26709..27341)
                     /locus_tag="VFV99_20970"
     CDS             complement(26709..27341)
                     /locus_tag="VFV99_20970"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5061859.1"
                     /translation="MSAVRPVGFFPLPGARQQGPRPIIVIHGYAMNRANFLPLAYRLA
                     RKGLGPIFGFEYWTLGRVAAGARQLAWFVEQVQAVTGSPQVDIVGHSMGGVVGRYFVQ
                     LLGGDGAVANLITLGSPHIGTDVSEFGIGHPTRELVIGSTLVQRLAAAPPPKDTRVTM
                     IFSHADALVPASTQDALEIPRSERIVYDDLGHVSLLGSRRVAREIITRLK"
     gene            complement(27531..>27762)
                     /locus_tag="VFV99_20975"
     CDS             complement(27531..>27762)
                     /locus_tag="VFV99_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061860.1"
                     /translation="AATTFLDQRVDLPDLYMKNLNPESRRGIYRAACRMAVVDHVFAH
                     AERKMLDRLQGILGIPLEIAQEIEADVPGMEV"
BASE COUNT         5022 a         9293 c         9017 g         4430 t
ORIGIN      
        1 tcgaaacccg atgcggagaa gttgcgacgc gcacgtgcgt gcgctttcgc ggcgacgtcg
       61 atcgcgcaga cctcggtcgc gccacccgcg tgcgagtaaa ccgagatcgc gccggtgtac
      121 gagaacagat tcagcacgcg ccgatccttc gcccactggc gaacggcgcg acgaccctcg
      181 cgcagatcgg cgaacaggcc ggtcgacagc ggcgcggtga catcgacgac gaacgacaga
      241 tcatcttcga tcacctcgac ctcgaccggg gccggattac cgcggacgag ctcggcggcg
      301 gcctgacgcg gcgcttcgcc cgcgagcgac cgatagcggc gttgctcgta gatcgctttc
      361 ggcgcgagct cctcttcgag cgcgccgtag aagtcatcgc gcaggttcgc gaccgacgct
      421 gaatagagct gcgcgacgag atagtcgccg tagcgctcga cgacgaagcc cggcaagcca
      481 tcggactcgc cattgacgag ccgcacgcag ccgagcttcg cgagatcgac gacgcgcttg
      541 cgcagtgcga tcgccgcgcg cacgcgctcg cggatcagcc ggccatcgat cgcgacgtcc
      601 ggtttccgga caaacacgcg aagcgcgatg gcgctgtccg cgtcgtagag cgcacggccg
      661 acgaattcac cgtcgggatc gatgaggtcg atctgcgtgc cgggttcagg cgcgatcgga
      721 cgtggaccga gcgcctctcg gtaaacccac ggatgaccga ggcggacgcg ggacgcgaca
      781 tcgcccgtca ggcggaccgt gttcttgcga acaatgggcg cgccgcggac gggccgacga
      841 ggagatctgc gggtttgcgc cataaagttt cggtagacag acctcctacc ccacgagtag
      901 tgtctgccgc aataagcggc cgtatgaacg atcctcgtcc tctcgcaacg ctcgaacctg
      961 ttcgatttgg tgagttcctg ctccaccgca acttgatcac ggatgaccag tggctgtgcg
     1021 ccctggccca gcactggagc gctagcagtt cgggacggat gcccattggc tcgacgattg
     1081 tggccaatgg tttcctcgct cgcgacgtcg tagagcggga agctcgcatg tttcacgagg
     1141 acatcgaggt cgtcgaggtg attccgcgct cggagcggaa cactatgccg ctgcctaacg
     1201 ttccccgcgc ctgaacaaaa gaatcgcggt gaggggccgc gattcgttct atgctctcgc
     1261 cccgtacaac cggagtccca tgatggctgt cgattcgaaa cagggtggtt cgtctgtgcc
     1321 ggcaaccgga acgccgtcgg cggggacacc gagtgcggcg cagacgctgg cgggcatgcc
     1381 cgcggtggcg gcgaacggca aaaacaagcg caagcggccg tcgtcgctgc tcgcgcaagc
     1441 gacgtcgttg ccttcggtcg agagctcgct cgatgacttc atcgcgcgcg cgaatgaaac
     1501 gctcggcgac gcgaacttcg acgcggcccg catcgcagag aaagagctca agcaagaaga
     1561 cgagaagcgc aaggaagccg acgcgctccg ttggaaggcg accgagcagc agatgcgcga
     1621 gagccaggcg cgcgaagagt cgctgcgccg tcagctcgac ggcctgcaag gcaagctcgc
     1681 cgaggccgaa gcgcgcgccg cggtcgcgag ctccggtggc tcgcaggacg gcgtgatcgc
     1741 cgatctcaag taccgcctca actcgctcga ggaatcgaag cgcagcgccg aagatcgctc
     1801 cgcgcagctc gcacaggagc tcgtgctcgc gaaggcgtcg caagccgccg cgcctgccgc
     1861 accgtttgtc agcgagtcgt tctcgaacgc cgacaccgag gagctcgagc agcgcgcccg
     1921 tctcgccgaa gcgaaggccg cgaaggcgct cgctgctgcg aaggctgccg ccgcgggtct
     1981 caccgtgaac cccgccgacc tcgccgcgat cgagagtggc ctggtcgtcc cgatggagac
     2041 gacgaaaaag ggcaccaact ggggcctcgt gttcgttgca ctgctggtcg gtggcgcgat
     2101 cgccgccgct gcggtcttcg tcctcaacaa gaaggacact ggcagcaagc cggctgctgc
     2161 gtcggtcgct ccgaccgagc aagcacagcc gccagccgcc gcgcccgccc ctgcgccctc
     2221 gaagccgacg gtgacgccga tcgaggagcc cgcagccgct cagcccgccc agccggaggc
     2281 agttgctgcc cccgccgccg cgccggaagc caaggcgacg cccgcgccgg tggagaccaa
     2341 ggccgagcct gccaaggtcg aggccaaggc ggaacccgct ccggccgagc cagccaaggt
     2401 cgagaagaag gcggctgcgc ccgccaagaa ggccgccgcg ccgaccaaga aggccgcgac
     2461 caagacctcc aagccgtcgg gcggccttgc cgacccgttc ggtggtggtg agaccgcccc
     2521 ggcgcccaag aagaccaagc cggcaaagaa ggccgccggc ggtggtggcg atacaggcat
     2581 cgtcgacccg ttctagccgc tcggccgcga atatctcgcg cacacagcac gccacacccg
     2641 gcgtgctgtt tcgttttcgg ttcgtttttg gcgcattgtg caccgcgttt cgcatgtgac
     2701 gcgcccaacg tttttccgac gacgaagtgc gctcggtgca gtgcgtcgat ctcacaaact
     2761 gatctcggat cttcgtcgtt gcatcgaaga taacgccgcg atattgaacc tcccgctaca
     2821 cgcgcaccgc gtagcggggg ttcctcctgt gaaacgcagc atcattctct gcatcgccat
     2881 cgcgctcggc actaccgcgc tcacgtcgtc tgcgtgggcc gaagatccag cgccgagcaa
     2941 agagcaactc gaagccgcaa agaaagcttt cggcgagggc aaggctctct acgacgagca
     3001 aaagtttgcc gaggccgtcg agaagttcaa agagtcgtat cggctctcgc gcaacccgct
     3061 gctcctctac aacatcgggc acacgctcga tcaggcgggg gcgaaggaca aggcgctgtt
     3121 ctactaccgc aagttcctcg ccgatgcgcc gccgaacgct gcgcagcgca aggacgtgca
     3181 gaagcgcgtc gaggtgatcg agaaggagaa cctcgaagcc gatctcaacg gcacgtcggt
     3241 gacgacgacg cccgcgaccg caacgacgcc gaccggaccg aagcccgaag agaagaaggt
     3301 cgtccagatc aagccggccg gcacgtactc ggagaaggac ttccagcacc agctcgtcga
     3361 ggccgcgcct ccgggcaagc cgctcgacat caccgcgttc gtccccgagg actcgggctt
     3421 cgtggtcacg ctgtactacc gcggcagcgg cgacgcgaag ttcatcgcga agccgatgaa
     3481 gtggcgttac aaggagctcg tcggacgcgt gccggcaaac aaggtctacg gcagctcgat
     3541 ccagtactac atcgaggtca aggatcaggc cggcaccgcc gtcgcgaagt ctggtaagtc
     3601 gacgagcccg aacctcatca acatcgagca aggcgcgacg ccgcgcttct accctgacct
     3661 cacggacgac ggcgagcagg cgatgaccgc cgcgcaggtc aagcaccatg acgaggagga
     3721 tccgatcctc ggcaagcagg gttccaaaca agaagtcgac accgaaggcg acacgcacaa
     3781 ggacacgacg gaagagcaac agctcacgac cacaccgccc ggtggtggca atggcttcac
     3841 cgatgtcggc tcgtcgaagt tcaccaaggc gaagtggatc tcgaccggcg ctgccggcgt
     3901 gttcatcggc gccgcggtcg cgacgtacat catggccaag aagcaggcgt cgaacctcga
     3961 agccgacacg atgtgcggca tgacgccgcc gccgtgccgt gcgttcgata aggactacga
     4021 cctcgcggtc caggatgcgg gcaagcgcta caacacgtgg cacaaggcga cgacggtgct
     4081 cggcattggc gccgccggtg tcgccgcgta cttttggtac aaggagctca ccgcgaagaa
     4141 gcacggcgag ctgaaggtca cgaacaagaa cgcgtctccg gagacgacct gggcgatcac
     4201 gccgtcgatc acggatggct tcacgggcgc cgcagccgcg gcgagcttct gaggatcgac
     4261 atgcgtagcc tattcttctt cgctctcgtt ggtctcccca tttgggccac ggcatgcacg
     4321 ccgtacgatc cggatctcgg catgtcgccg ttcctctgcg gcccggcgga tcagaacccg
     4381 cgctgccccg acggctacac ctgcgagatg acgtcgtcgg gcgagtactg cctcgcgatg
     4441 ggtggcatgg ttccggtcga cgcctcgaac gtcaactgcg ccgacgacag cgcgctcgag
     4501 ccgaatgatt cgatcgcgca ggcattccag acgccggtcg cgacgaccaa gaacacgctc
     4561 acgttcgcgg gcctcgcgat ctgcccgatg ggcgacaagg acgactactc ggtcaccatc
     4621 acgaccgcga accagaacct cgaggtgatc atcgagtacg acgccgtcgc gggcgccgac
     4681 cttcagggct cgatcctcaa ctcgggtggc caggcgatcg cgaacgcgtc gccgatgggc
     4741 accggcatgc gtcgcgcgta cacgccgaac ctgccgaccg gcgtctacta cgcgtcggtg
     4801 tacggcccga catcgggcgc gctgcagacg aacaactaca agctcacgat caccgtcact
     4861 ggtccgtgat gctcacgcgc cggcaggcgg aagcatcgcc ggcagcggga ccgtgaacag
     4921 atcgtccatc gtcggcgcat cggcgctggc agccgtgccg cacggcagcg gctcgacacc
     4981 ttcgatcgcc tgcacggtct tgaggaacga gctggtgtcg tacggcgtgt tcgacacgta
     5041 gcccttcttc gcgagcggtg acacgacgac gaacggcgga tcgtcttctt ggctccagtc
     5101 ggtcgcggag cggccttcgc cttcgtcggc cgtgatgatc aggacaccgc cgttctgcca
     5161 cgccttcgat gcgaggatct tcggcacctc gcgcgccagc cacgcatcgc cctgcgcgat
     5221 cgtgccgtcg tgcatgtcgt tgatgaggtt cggcgtgatg aacacgtagt cgggcacggt
     5281 gtcgttcgcg agatccttct cgagctcgga gtagtcgacg acgtgatcct tgcagcgctg
     5341 ctggcgcagc ggcgtcttgc cgttccagcc cacgacgtca tcgaagaaca cgaacggatt
     5401 gtgcttcggt gcgtacttgc cgtcgttgac gatcttgcac ggctcgccca tcgattcctg
     5461 ataggtcttc cacgtgcgac cgacgccctc gagctggtcc gcgaggtgcg aggtcgacgc
     5521 gatgtgatgc tccgccggtt cgccgtcatc gagaatgccg aagttctgcc ccgacaccat
     5581 ccagatgtag ttcggctcgc tcggatgaac tcgcgcgtcg gtgtagccgt tcgcgatcgt
     5641 gtactggtgc gcgagcgatt tcagataggg cgccgcggtg ccctcgagca gttgcgcacg
     5701 cgacttgttc tcgatcacga tcgtgaagac cgtgccctga aacggcgact cgagtgctgc
     5761 agttgccggc gacgtcgcag tcagcgctcg acacgcttgc cccttgacgt tgccactggt
     5821 gtcgacgcta caggcggagg ctgcgaaagc gaccgccaga agacctactg tgacgcgcat
     5881 cagcgcactc tgatgcgttt tcgcgccccg gcaatcagga ggtatcgcag tcttatgcga
     5941 cttgcagctt gatggcccac cgcttcactt cggcggtgag ctcgttgtcg ttgccggcga
     6001 cctgcgacag cagggcaagc gcgactccag cgcccgcata atcgtgactt tcccagcacg
     6061 tcctcagctc tagcctgaga agcgcgatct gctttgcttc ggcgtagctc atcggatcct
     6121 ccaagaaatc gcccatcgtc aaattcaggg gctcttcgag aacccgcaac tttttggacg
     6181 cacgaggcgc aaaaagattt cgtcgccagc gtcgggcagc agtggggtcc ccgcctgccg
     6241 ggtggtttga cggatccccc aattgcggag taccttgtcg aaaacccgag cttttttggg
     6301 agaaccaccg gtatgagccg tattggagag ctactggtcc gcgagaagat gctctcgctc
     6361 cagcagctgc agcaggcgca agacgaagcc aagcgcaccg gcaagcgcct gggtgcgacg
     6421 ttggcgcgtc ttggctatgt cgatgacacg cgactgaccc aattcgtcgc gaagcagtac
     6481 agcctgccgt cgatcaacct gtccgagatc gagatcgatc cggcggtcct caagctcatc
     6541 cctcgtgaga tctgcgagaa gcatcaggtc attccgatcc gccgcaacgg cccgacgctg
     6601 gtcgtcgcga tggcggatcc gtcgaacatc tacgcgatcg acgagctgaa gttcctcacg
     6661 cagtacaaca tcgaaccgat cgtcacgtcg gagtcgggcc tcgaggccgc actctcccgc
     6721 tactacgaca agggtcccga cctcgaccag atggtcggcg agctcgatgt cgaagacgtc
     6781 gacttcgcgt cgatgacgga cgagaacgtc aacgtcgtcg atctcgagaa ccaggccggc
     6841 gaggcgccgg tcgtcaagct ctgcaacgcg atcctcctgt cggcgatcaa gaagaaggcg
     6901 tcggatatcc acgtcgagcc gtacgagaag agcctgcgcg tgcgcttccg catcgacggc
     6961 atcctgcaag aggaaatgcg gccgccgctc aagctccgca acgcgatcac gtcgcgtctc
     7021 aagatcatgg cgtcgctcga tatcgccgag cgccggctcc cgcaggacgg tcgcatcaag
     7081 ctcaagatcg gcggcaacaa ggagatggac ttccgcgtct ccgtgctgcc gacgctgtac
     7141 ggcgagaaga tcgtcatgcg tcttctcgac aaatcgaacc tgcagctcga catgacgaag
     7201 ctcggcttcg atccgaagcc gctcaacgac ttcaaggtcg cgatcaagaa gccctacggc
     7261 atggtgctcg tcaccgggcc caccggttcc ggcaagacca cgacgctgta ctcggcgctc
     7321 tccgagctca acacgatcga cacgaacatc tccaccgccg aagacccggt cgagttcaac
     7381 ctgcacggca tcaaccaggt gcagatgcac gaggacatcg gcctgaactt cgcgaccgca
     7441 ctgcgcgcgt ttctccggca ggacccgaac atcatcatgg tcggcgagat tcgtgacttc
     7501 gagaccgccg agatcggcgt gaaggcagcg ctcaccggcc acttggtgct gtcgacgctg
     7561 cacacgaacg acgcgccgtc gacggtgtcg cgtctcctca acatgggcat cgagccgttc
     7621 ctcgtgaccg cgtcggtcaa cctcgtgctc gcccagcgtc tcgcccgccg catctgcgcc
     7681 gactgcaaga tcccggcgga gcgccacgtc gaagcgctgc tcaagatggg catgaccgag
     7741 gagcaggccc gcaagggaac gatcatgaag ggcaagggct gcggcacgtg cagccagacc
     7801 ggctacaagg gccgcgtcgc gctctacgaa gtgatgccgt tcaccgaccc gctgaaggag
     7861 ctcgtgctcc agggcgcatc ggcggcggag atcaagaccg agatgatccg cggcggcacg
     7921 cagagcctcc gcctcgccgg catccagaag attctcgaag gcgtcacgac acccgaagag
     7981 gtgcttcgca ccacggtgga tgactagtgg ctcaactaaa cctccaggcg ctgctcaagg
     8041 cgatgctcga caaaggagcg tcggatctcc acgtcaccgc gggctcgccg ccgcgtctgc
     8101 gcatcgacaa cgagctcgtg cgcctgcaga ccgatccgct cggtccagtc gacaccaaga
     8161 cgctctgcta ctcggtgatg aacgacgcac agaagctccg gttcgaggaa gacctcgaga
     8221 tcgacttctc gttcggtatt cgtgggctcg cgcgcttccg cgcgaacgtc tacatgcagc
     8281 agtcgtgcgt cgcaggcgcg ttccgcctcg tgccgtacca gatcattccg ctcgaggagc
     8341 tgggcctgcc cggcgtcgtg tcggacctgt gcgagaagcc gcgcggcctc gtgctggtga
     8401 ccggaccgac cggctccggt aagtcgacga cgctcgcgtc gatgatcgat cgcatcaaca
     8461 cgcggatgcg cggtcacatc gtcaccgtcg aggatccgat cgagttccag cacagccaca
     8521 aggcatgcct cgtcaaccag cgcgagatcg gccgcgacag ccaatcgttc aagcgcgcgc
     8581 tcaagtacat cctgcgccag gatcccgatg tcgtgctgat cggtgagatg cgcgacctcg
     8641 agacgatcga ggcggcgctc gccgtcgccg aaaccggcca cctctgcttc ggcacgctgc
     8701 acacgaactc cgcgatccag agcatcaacc gtatcgtcga cgtgttcccg tcgcaccagc
     8761 aggagcaggt gcgcgccgtg ctgtcgttcg tgctcgaagg cgtggtctcg cagacgctga
     8821 ttccgaagag caacggcgtc ggccgcacgc tcgccgccga agtcatgatt ccgaacgccg
     8881 cgatccggaa cctgatccgc gaagacaagc tgcaccagat ctattcgcag atgcagatcg
     8941 gccagtcgaa gttcggcatg cagacgatga accaggcgct ctgcgacctc tacatccgca
     9001 aagtgatctc gctcgaggat gcgctcggcc acacgagcga gctcgacgag ctcaagacga
     9061 tgatcctgaa cgccggcggt tcgctcggac agcactccgg gccggccacc gctccaaccc
     9121 gacgcagctg aggagctgag cgatggccaa gttcaactgg gaaggtacga cgcgggcggg
     9181 tgagaagcgt cgtggcgtca tggaggcgga gacccgcgcg gtggtcgaag agcgcctccg
     9241 cagcgacaac atcaatatcc agagcgtgaa gcgcgagggc ggcctcggcg acatccagat
     9301 ccagatcggc acgggcgtca gcgcgaagga cctccagatc ttcacgcgtc agctcgccac
     9361 gatgatcgac gccggtctgc cgctcgtgca gtgcctcgac atcctcgcgg cacagagcga
     9421 gaacaagtcg ttcaagaaga tcctcacggc cgtcaagagc tcggtcgagc aaggcgccac
     9481 gttctccgac gcgctccgca agcacccgaa ggtgttcgac gagctgtacg tcaacctcgt
     9541 cgcagccggc gagctcggcg gcatcctcga tacgatcttg aaccgcctcg cgacgtacat
     9601 cgaaaaggcc gtgaagctgc gcggccagct caagagcgcg atgtactacc cgatcggtat
     9661 tctcgtcgtc gcgatcggcg tcatcgcggt catgctcgtc aaagtcattc cgacgttcga
     9721 agcgatgtac cacgacatgg gcaacgccga gctgccggcg gcgacgcgct tcgtcatcaa
     9781 cctgtctcac tcgttcttga acaagtggta catcttcgcg gggacgttgt tcggtctcgt
     9841 gttcggcacg atcgccgcgc gccgcactga cggaggtcgc gaagcgatcg atcgcatgct
     9901 gttacgcatg ccggtcatcg gctcggtgtt acgcaagatc gtcgtcgcgc gtttcacacg
     9961 cacgctcggt acgttgctcg cctctggtgt gccgatcctc gacgcgctcg atatctgcgc
    10021 gcgcacctcg ggtaaccgcg tcgtccaggc cggcatcctg cgcgcgaagg acaagatctc
    10081 cgaaggtcac gacatggcga gcccgctcgc cgagtcgaag gtgttcccga cgatggtcgt
    10141 ccagatgatc ggcgtcggtg aacagaccgg cgcgatggac cagatgctcc agaagatcgc
    10201 cgacttctac gaagaggaag tcgacaccgc ggtcgccggc ctcacctcgc tgatcgagcc
    10261 ggtgatgatg gcgttcctcg gcgtcgtcgt cggtggtctg atcatcgcga tgtacctgcc
    10321 gatcttcaaa ctggccggta acatccaagg atgacgggag ccacgatcgg ggttcgcctc
    10381 acgacgccag atgcgtcgcc gggcgattcc ctggtccgcg ctcctgccct gcctgatagt
    10441 gacgtccgcc ggcgcgtgac gcgcctcctg ctgctgcgca cgattgtcgt gagcgtcgta
    10501 ctcgggctct cgctgctcgc actcgtgcgc ggcgaagcgc ccgcacgggc cgcggtatgg
    10561 ctgcaatcgg cgatcatcgc ggcgacgtat ctgtcgtcga tcgtgttcgg cgtcctcttg
    10621 cgccaaggct tctcgccaaa gcgcgtcgcg cggccgatga tggcgaacga tctcgtactg
    10681 acgagcgcgc tcgtctacgt cacgggcggc gcgatgagcc cgtacacgtt cctgtacgcg
    10741 ctgacgatcg tcgccgcggg tgcgctctcg taccggcgcg gcgcggtcat cgtgaccctc
    10801 gcgtcgctcc tgctcgtgct cgtcgtgtcg ctgctcgcgt ggcatcgcac gatcgacctg
    10861 ccgctgtccg cacaggtgcg gccgtgggag caatcgaccg ccgagctcgt gcgcacgctc
    10921 ggcatcaacg tcgccgcact gatcggcgtc ggcgcgctca gcttcatctt cggcgaccag
    10981 ctgcagaaga gcgccgacac gctcgcgacg acgcgccgcg cggccgccga gctgctgacg
    11041 ctgcaccaag acatcgtgcg ctcgctgagc tcgggcctca tcacgacgac acccgacggt
    11101 cagatcctca ccacgaacca ggcggccatc gacatcctgc gcacgacggc aggcggcctg
    11161 atcggccggc cgatcgattc gatcatgccc ggcctcaccg ctctgctcac aaagaacaag
    11221 ggcgagctgc gccgcgccga cctcacggta ccgacctccg acgacaatcg cacgatcggt
    11281 gtcacggtct cgccgctgcg cgacgtccgc gacgagatcg tcggttgcgt gatcaacttc
    11341 caggatctca ccgagctgaa gcgcctcgag atgcacgcgc gccgcaccga gcgcatggcg
    11401 accgttggcc agctcgcagc cggtgtcgcg cacgagatcc gcaatccgat ggccgcgatc
    11461 tcgggctcga tcgagctgct caagcagtcg tccgaaacga gcgacgacga tcgcgcgctg
    11521 atgacgatcg tgcagcgcga ggtccagcgc ctcaacgtgc tgatcggcga cctgctcgac
    11581 tacgcaaacc cgcgccccaa acaagccgtc gatttcgatc tcggctcgct cgtccaggag
    11641 accgtgcagg tcgcgcgcgg cgaccagaac ttctcggaga tcgagatcac cgctgccacc
    11701 gacgaaccgc tcgcgatcca cgccgacccc gcgaagctgc gccaggtcgt gtggaacctc
    11761 gtgcgcaacg cagccgacgc ggcgggcggc ggcggcaagc acgtgaaggt cgaggcgcgc
    11821 cgcgatggcg ggggcgcgaa gatcagcgtc gccgacgacg gccccggcat tcccgacgcc
    11881 gtgctcggcc gcatcttcga tccgttcgtg accacgaagc agaaaggtac cggcctcggc
    11941 ctcgccacgt gccactcgat catcaccgag cacggcggcc gcatcgacgt cgagacggcg
    12001 cccggtaagg gcaccaagat ggtggttacg atcccgccgc cgcgttagtc tggctcggtg
    12061 aactgcccga tctgcacgca agcgctcgag ggtggcgagc tgctcatggt ctgcacgacg
    12121 tgccacaaga acctcggcag cacgagccta tcggtcggtg caaccggcga gttccgcgtg
    12181 ccaacgccgg agatgattgc cgccgtcgct gccgccgagc caggcgagcg tcccgatgcg
    12241 accgacctct gctcgtggtg cggcaaggcc gagcaccaag tgaagaagct gctgggccgc
    12301 agcggcactg cgctgtgcaa cgagtgcgtg tcgctcgcgt gcgacatcat ggacgccgag
    12361 ctcggcacca actggcgcta gcgcgcgagc gcctcggcgc gcagccgata atcctctgca
    12421 tacgcggctt cgcccttctg cgaccacgcc ttcgcgagat cgcgacaccg ttcggcctcg
    12481 aggcgtgccg gtagctcacc tcccgcgcgc ataccgtcga gcaccgcgat cgcatcgtcg
    12541 acgcgaccga cggcgagcag cgccatgccg cgcgtgtgct gcagcgcggg cacgtccgga
    12601 cggagtgcac tcgcttcatc gacgagcacg agtatgtcct ttggatcacg tccggcagcg
    12661 agcgcggcgc acgcgcgatt attcagccac accgcgcgct cggcggcggc gagttgcgcg
    12721 gcgtcgatgc tcgcgagcaa gcgctcggca gccgcgagct ggtcactcgc gatcagacaa
    12781 ccggcgcgcg acagcagcgc ggcgcgttcg cgaatcgccg acggtgcgag catgccgagc
    12841 aagcgatagc gacgctgcgc ggccgtgtac ttaccggtct cgaacgcgtc gtgcgcggag
    12901 cgcggtaacc acagccacag ccagaacacc gcgacgaggc ctagcccgat ccccgcgaac
    12961 gtgcccggac ggccgagcgt gttgccgccg acaaacggca gcaacaacac gacgatggca
    13021 acgccgagga tgcgggcaac gaagcgcatc gcgccgagaa tagccgactc ccgagcaacc
    13081 ggtttgcgag aaactcggtc cgatgtccgt gcgcaccgtc cccgtgctgc tgatctccgt
    13141 gatcacgcac ggcgcgttgc tcgcgctcgt cgcggactgg ctgacgcctg tgccgacgca
    13201 acggcttgca tggcgcccag tcaccgaacc gccgccgccg ccagtccacc tgccgagggc
    13261 aacggccgaa ccgatcgaga tcgcgttcct tgccgacgag ccggtgacgg ttgcagctgc
    13321 ggatcccgta cacgagcgtg cgccggcggg agctgccgcg gtgcgcacgc cggggagcgc
    13381 cgcgatcacg atgaccgcga catccggtgg cacgagcgaa agcgggcgcg agcccgcacc
    13441 tggcggcgcc gggcccgcga cgagcgggtt catgcacatg cgaggtccgg atctcggact
    13501 ctccgcgggt accgccgagc gcatcgcgag cgaaccgcag aaaccgcgcg acgagctcca
    13561 caagtcgggc aagctcgaga gtcagcccgg tggccgcgcc gtgatctacg accgcgtgac
    13621 gaccgtgagt gtcgatgccg atggcacagc gcacttcgac gacaagaagg acatcgatct
    13681 ccacttcaag ctgccgattc ccaagatctg ggagatcgag gagatgcgca aagacctcgg
    13741 ccacgagctc acggagtggt tcaaggatcc cgaagccggc aagcgctacg gccgcatgca
    13801 agatgtcgcg cgtcacctga ccgcggttga aggcgcgtgc gacagctggg gcgacttgat
    13861 gtgcgacgac ccgctcgcac cgcagatcga gaaacgtgtg cgcgaacgca agccgaaggg
    13921 cggccgcggc ttcatcggca cgtttctcgc cggcccgatg gacatcacgg cgtggctgca
    13981 tcgcaagtac gtcggcgatc cgtatgcgtc gcgcaagctc aagcttctcg acgacacgcg
    14041 cgacgagcgc gtcgcgatgg gcatcgcgca ccgccagcag cagagcgtgc gcagcgccga
    14101 gtacatgacg cgcaatctcc aggcgctgtg ggcgcgcgag caatcgccag tcgcgcgccg
    14161 cgccgcgctg ttcgagatgt gggacgaatg cgacgaggac gacgccggct tgcgcgcccg
    14221 cgcagtcgtg atcggctgga tccgttcgca cctgcccgcc ggctccgccg acgcgtacac
    14281 cgaagccgag ctcgctgcgc tgcaatcgca caagggctcc agcgccgagt tcgcgcccta
    14341 cgcagacgtc gacctcagca tcacgaagcg cggcgaataa cgctgttcgg gcgccttaga
    14401 acaaccagcg gagaccgagc tggatcgcgc gggggtcttg gcggccagac gtgtgcgcga
    14461 agttcgggtt cttcttgatg actcgattct gttcggtaaa gtcgctcgga tcgatcgcct
    14521 tgacgtgctc gaggtcctcg acgataccgc ccaccacctg gttcgcggtg tcgagcgtgt
    14581 agcgctgatc cacagcggtc tcgctctgcg catcgaacag attgaacacg tcgacgaacg
    14641 cctcgagcga cgtcgacttc agcaagccgc gtgcgaacgc gaggtggaca tcgagctgac
    14701 cggtgacggg cgtgcgcccc atgctgccgg ccgccaggag atacgactcg ccgccgccgt
    14761 acgtcgggtg cgtcgcgagc gcgctgtatg cgatgcccga ttgcacgcgc acgctggcgc
    14821 cgaccgtgag tgccgtcgct ggagcgagat cgaaccggta gaagccgtcc gcatgcagct
    14881 tgtgcggtcg atccagcccg aggatgccgg tccggttcgg cagcacgctc ggcagatcga
    14941 agaacgcgtt gaggttcgga aggagctgcc cggtctccgg agagaacagg ccgggatagt
    15001 tgccgcgatt cgcggagtac gtgtacgagg cgagcaggag cgcgcgcggc gtcggcctca
    15061 gcgttgcaac gagctgaagt gcattgtagg tgcgaaccgg tttgtcgagc tgatcgacat
    15121 cgtcgagcca ctgcgcacgc gtctcgtagg tctcggcgag ctggcggtcg cgtagctcat
    15181 tcgagcccgc gagctccgct gcgcgtgcgt ggagctcggc cgcctcggca tcgaagctct
    15241 cacccggatt cgtgatgaac agcgtgttgc cgccatccat cgacacgtcc tcgatcacca
    15301 cgggtacgat gcggtgctga aagcctgccg cgaccttcaa atcgggcctc acttcgtact
    15361 cggctccgag cacgatctcg tcggtgtact gaccgcgcaa gccaggcgac acgaactcga
    15421 aggcttgcag gcccgcacgc gcactgcggt ccgtgcacgc gcggatcgtg tcggtaagat
    15481 tcggcgtgtc gtggtcgacg gtgcagttcg gatcgtatcg aggtgtgttc gcttgcagac
    15541 gattcacgtt gagcgtcgtg cgcgtgaaca gcgcgttggc gaacagatcc gagccggtca
    15601 gcggtagcgt ttcgtagaac cgtccgtagt ggccgaacag cttcgcgcgg ccctcgcccg
    15661 tcggatcgtg gatggcgccg acacgcggtg cgaaattgtc gagcgcgaac acgcgcgcgc
    15721 ccacggtctc tccctccggc gtcaccgtcc cgacgacgtc gtcagcgacg tagccagcct
    15781 gatgatccca gcgcacgccc gcgttgagcg tgaagctcgg cgtgatcagc cagctgtcct
    15841 gcaggtacgt gccgatgtcg acctgcgacg tgctcgtatg aaccggatca gcaagcgcac
    15901 acaccgcatc gcggtcgggg cagacctcct gtccggcacc gagcatgatc gagctgggat
    15961 tcgcacgttc ttcggcggtc aggttacgaa cgacgcgcat cgtttcggtc agctgccagc
    16021 gacctggcgc gcccgcgcga ctatcggcac tagcccgcgc gagcgcgtaa ccaccggtga
    16081 agtgatatga cgtgcgatca cgtgcgcgct cgagctcgct accgagcttg atcgtgtgat
    16141 agccggcgag cttcgcgcgg tgcgtgaccg acaacgcgag cgtcgcgcgg tcgtcgcggc
    16201 gcggcgcgac cacgccgagg ccttgctccg cgtaaccgaa aaccgggcag ttcacgatct
    16261 ttggatagag gtccgttccg tcgtcgacga tgctgtcgcg acacgcgtcg agattctcgg
    16321 tctcgacatc ggcgaaatca gcgagcgagc gatcgtacgc gtaacgcacc tgcggccgct
    16381 cgtcgcgcgc gtgcggcagg tcataggtac gatgaaagcc ggcggtcgcg tcgagctggg
    16441 tggcggcggc gagcttcgag gtccacttgg cggcgaggtt gtacgagccg aagtcgtgat
    16501 cccacacgag cgtcgccggt gcgcgcacga ttccgaatgg aatctcggtc gccgtcgacg
    16561 gattcccgaa cgcgacgacc tctccctgat ggtgctcgtt gaccgcgccc gtcagtttcg
    16621 ccgtgaagta atacgtggtg ctgtggagcg gcagcgaggt ggtcgcgacg tgctcgtgca
    16681 gcgtgtactc ggtcgtcgga tcgacatcgg gcacgccgtc ctgatcgcgg tcgacctggt
    16741 gcgcgatgat tcgctcacga gtttcgtggc tgacgctcgg catgaacccc gcgtgaaacc
    16801 acaggcgctt gggaacgagt ggtccgccga cctcgccacc gacgtcgtag ctgtcgcctc
    16861 ggaccgtgcg tgtgtcgatc gcactgccct cgcgctggat cgtcgccgcc ttcgcagcga
    16921 gcgcggctgg cgtgtagtga gcgaatacgg acccgtgaaa cgcgtcgctg ccgcgcttcg
    16981 tgatcacgtt gacgacgcca ccggtcgaac gtccgaactc ggcgtcgtag ccgcctgtga
    17041 tcacctcggt ctgcgagatg aattccgtcg gcaactgcgt cgacagcgcg ccgagcagcg
    17101 ggtccgtggc ggcgatgccg tcgacgtaat acgcggtttc cggcgacgtc gtgcctgcga
    17161 tcgagacacc gtagcggtcg ttctgtgcgc ctgcgacggc gccgacggta tcgccgaaca
    17221 gccgacccgt cggaatgttg cgcgtgtact ccggcgtcaa cacgacgccg agcttggtcg
    17281 agctctgatc gacgaccggt gcctggccct ggatcgcaat gacctcgccg ggctcggcgc
    17341 cggtatcgag cgcgaagttg acgacgacct gtttgccgag ctggaccaac acctcgcgcc
    17401 gcgagaactg atggctgagg tagtagacgg tcagccggta cacgcccggt ggcagcgcgg
    17461 tcaaaaagta cgcgccgcgt tgatcggtga tcgcggtctg ctcgccctgc agcgactccg
    17521 acgtcgcgat cacgatcgcg cctgccgccg gaccaccgct cgtgcgatcg acgagctgac
    17581 cgcgcacact gccaaccgtc gccggttgcg cgtacacgac acgaaccgcg aacagcaaca
    17641 ccactacggc tctcgtgatc atgatggaaa gccgacctgc ttgaggagtg cctgaaagcg
    17701 gggatgggaa cgcatttgcg gcgagtaacg ctcgaccttg aggaacagcg cgccgatgtt
    17761 gtgactcgcg accatctgct cgagcagcgt gagcgaacct tcgagatcgc cgttcgcttc
    17821 ggcgagcgcc gctcccacca tgaaatcgat gttgtcgccg agcttctccc ggatcgcctt
    17881 cacaacgtcg gtcttgcccg ccgcgaggta gacggggagc aggacggcga gattgtcgtc
    17941 gtcgacatac ggtcgcagcg tctcggcaac ccgaaacgca tcatcgaatc gcgcaagctc
    18001 gacgtacgcg agtgggagcg ttgtggcgta cgtcgcctcg acaaagttcg tgaggtcgaa
    18061 gcgattcatc accggctcga gcacgcgaat cgcgtcggga tagcggcgcg cgtagaaata
    18121 cgcccacgcc agcgggccgt cgccgagacg cggattcggg ttgatacggt gttgcgtttc
    18181 gaagatcggt agcgcctcgt cgaagcgacc gaccaagtgc aggaagttgg aacggtgata
    18241 ggcgacacgc tcatgcaacg gcgccagctc gaagccgcgt ttggtttctt cgtcggcgcg
    18301 cttccagtcc cagcggatca tcgcggcgtg accgcgcacc gcatgcatca gtgcatcccg
    18361 tgggtccgcc gcaatcgctt cgtcggtcag tttgtcgacg atcgtcaggg tcttctcgac
    18421 cttcgtgtag tcgttgcgct gctccgcggc catcacgagc cgatcggcgg cgatgtcttg
    18481 cgccctcgcg agtcgcggct gggcttcgac gagatctgcg agctgtgtga atggagtcgc
    18541 cattcgcttc tgggtgaaag cctcgatttc ctcgggggtc ggctgccggt acatccggcc
    18601 gaccgtatcg ccgtagtgcg tatagccatc agcagcaatg tggcgcgcat cgacgggtgc
    18661 gatcggaggc gcgtagccaa gacgacctgc gatcgcagtc gccgcgtcgt gaaccacgag
    18721 cgcgacctcg gtgagcggac gccgcagcgt ctggctccag ctctcgcgtc cgcgtgccgt
    18781 gactgcgagc tcgacgtgcg tcgcagtgcg cgcaatcgtc attgtcacgg ccgtcaccac
    18841 atggggaacg gcgagagcgg cagcgacggg atccttttcg tcgagcaggc gcgacgcggc
    18901 accattcaac gtggcatcca tcgcgccggt atggagagcg agcacaggca ttccgagctg
    18961 gacgagtgcg gagtgcagat cgaacgtgat cgcagtgcgc cacgagtcct tgatccaagg
    19021 ccccgcgccg agcaccggca gcaccgcgat gagatcgtcc ggcgagcgcg tgaccgcggg
    19081 cgtattcgca ccgctcgacg ccgcgaattt ccatacgccg agcgccgccg ctgcgatgcc
    19141 acctccgccg acggcgagaa accagcgccg cgaaaccggc ttgtgaccga ggagctcgtc
    19201 gacgctggcg aagcgcgccg ccggatcacg cgcaaggcag cgcagcacga tcgcatccca
    19261 gcgcgcgggg atgtcgctgc gcagcgacga tggccgcgga ggatccttcg tgagtcgcgc
    19321 ggtcgcagtt gccattgcag tgtcctcgtc gaacggcagc ttgccggtga gcagctcgaa
    19381 cagcacgaca ccaagtgagt agatgtcgct cgccggcgtt accgcgcgcg cctcgacttg
    19441 ctcgggagcc atatatgccg gcgtgccgag caagcccgac tcgacggtga ggcgagaatc
    19501 cttggcatcg accgaccgcg cgagaccgaa gtcggtgatc accgcccgat cgccaaccac
    19561 gatcacgttc gacgtcttga aatcacgatg gacgatcgac gcgcgatgca gcacgccgag
    19621 ccccgcgacg agctgcggcg cgatccgctc gatttcgtcg agcgccatcc cgcctgcctt
    19681 caccagctcg gcgagtgtcc tcccctccaa caactccatc gacaggaaca ctcgcccatc
    19741 gtgctcgccg acatcgtgca gccggcaaat gtgtggatcc gtaacgctgc gcgcgagagc
    19801 gatttcgcgt cgcagtcgcg cgatcgcatc ggccgatcct tcgagctcgg gtcgcagcgt
    19861 cttgagcgcg accttcgtgt cgagcaccag gtcgtgcgcg gcgtatacct cgcccatgcc
    19921 accggccgcg atgaaccgtt cgatgcgata acgctccgcc gcgagcgagc cgatcgcgaa
    19981 ccgttcggtc gcagcgtcgc tcgtcgacgc gtgcgacggc agcgtctcgt cgccgcccgg
    20041 tgcacccatt cttcgcacca taaccgatca cggctggctc gtgcgcacca gtgggatcgc
    20101 gatgtggctc ttctccgatt gcggctcgta cgcgagatcg agctgcttgc agacgcgaac
    20161 ggtttgcggc gccgagaacg cttcgaactg ccattgtgcg atgtcatgca tcgcggcgcg
    20221 gtcgagctcg gacgactcac tcggacgggt gatgtcgacc ttcgacacct tgccatccgg
    20281 cgcgacgcac agtcgaaccg caaacgtgta gcgatcgcga tcggtgctca gctcgacctg
    20341 acggtgggcg acgctcggca gcacggcgtc cgacgcgagc atcgggaacc acggcttcgc
    20401 ctcgtcggtc gactgcgcga gctgaccgtt gaacgtgggt ccgatgcccg tacccgacga
    20461 ctcgacgggc agcgaacgac cggtggtggc gcaggcggaa accaggatgc tgacagcgat
    20521 gatgttcttc atggtgatga cctccgtccg gtcgctagag cactcgcgat gccagcctag
    20581 acgtgcgtca tttcaatcag ttagcgaccc ggacgtcgcc atcggacgtc cgagccgcaa
    20641 gcgtcggacg cttgctactt cgcgagggcg agctcggccc agcgcagctt gagcgcgcgc
    20701 cgcgaaacct gaaggacttg cgcggccgca tcgagctcgc cggcgcagtc gtcgtacacg
    20761 cggcggacct cggcgtccgg cagatccttt gctttgcgga tcttcgggca ccgatcgatc
    20821 aagaggtaga ggctcgaccg agacacgccg agccgcgccg ccgcggctgc cgcactccag
    20881 ccgacactct gtagcgtcgc gatcagctcg tcatccgtga tcgccgatgg atcgcggcgc
    20941 gcctgcgcgg cgaccggcga gctcggtgcg ggcgcggtcg cagcgagcaa ccgctcgagt
    21001 gcactgtcgg cgcgtacctc actcgcgccg cgattcgcga tcgcgagctg gcgagcggcg
    21061 ttgcgaagct gacgcacgtt gcccggccac atgtgccgga cgaggcgcgc aacgagcgac
    21121 gagggcagcc agagatccgc atcgacttga cgtgcgagtc gatcggcgtc accagtcgac
    21181 acgagctcgt cgcgcaaaaa gtgaacgagc aagcgcccga tgtcgtcgcg acgatcgcgc
    21241 agcggcggta cgagcagctg gtagccggcg agccggtgaa acagtgcctc gcgaaatccc
    21301 gactcgctga tctgcagctc gagatcggca tcggtggccg cgatcagacg cacatcaact
    21361 ttgcgcgagc gcgcgctgcc gagcggcgtg acctcactcg tctcgagcac gcggagcagc
    21421 gacggctgga tctcgaatgg agtctcaccg acctcgtcga gaaacaacgt gccgccgtcg
    21481 gcgcgcgcga acatgccgtc gtgatcatgc gtcgcgccgg tgaatgcacc gcgcgcgtgg
    21541 ccaaacagct ccgacgcagc ggtcgtcggc ggcaacgcgg cgatgttgat cgcgaggaac
    21601 tggcggctcg cgcgcggact gctcgcgtga agtgcatgcg cgacgagctc cttgccgaca
    21661 ccggtttcgc cacgcacgag caccggaacc ggcaaatcgg cgacgcgcga gatgtcggcc
    21721 cgcacgcgct ccagcgaatc gctgtcgccg acgagcccgc agcgcggtgc ccgctcgcca
    21781 tccggcgcgg cgaggtggac gacgaggacg acgcgatctg cgatctcgag caccgcacca
    21841 cgctcgagcg ccgcctgatc gagcttctgc caggcacgcg cacccgcgcc tgcaagtgga
    21901 cgcccgtcca gctgcgccgg ggtcgtcgat gcaagctcga gcgcgacgcc ctcgccggac
    21961 cgcacgagct tgacgatcgg gttgcgcgac agcccgcgat cgccgagtcc tcgctgctcg
    22021 cggctgcccg gcgcgctgaa catcggtgtg ctgcgcgaga tgtcgaggtc gttgccctcc
    22081 agacgggcac gctcgccgac gcgtgagaca tcggggtgat acgcgatggt cagcgtgatg
    22141 acgtcaccgg cccgcgctgc gctgcgcggt gcagcgggca tcagcgtgct cgcgtcatcc
    22201 accatcgcgc gatggtaact cagctcgcgc cgtcccaggc atgcgcgcgc gggatcgcga
    22261 gctcgaggtc gccgcacgct cggcacaagg cagttgccat atttatgtat ctatagcaca
    22321 catatataca tatgagaacc agcatcgatc tcaacgatcg cctgctgacg ctggcgaagc
    22381 acgcggcgac gaagcgcggc atcacgttgc gcgaggtgtt cgagcaagca ctgcgcgcgt
    22441 atctggagcc atcgcgaaaa caggagggct acaagctcga gctcgggatc acccatggtg
    22501 aggtggtgcc cggcgtgccg gtcgatgact ggtccgcgtt gcgcggttac gtcgctgagc
    22561 tcgacaccgc agacgacttc aagaggtaca ggaagtgatc gcgatcgaca cgaacatcat
    22621 cgtctatgcg cgacgcgcgg aactgccgca tcatcgcgcc gcgcgcacgc tgttgaccca
    22681 gctcgctcaa ggggcgacac catgggcgat cccgtggcca tgcctttacg aatacatcaa
    22741 ggtcgtgacg aacccgcgcc tgttccggcc gcccgacacg ctcgcggacg ctctcgccga
    22801 cgtcgagtcc ctggtcgcct ctcctagcgt cgtgctcctc ggacacggcc ccgaacatgt
    22861 caggcatctg cgccgcgcgg ccgatgacgg ccacccgatc ggcggccaga ttcacgacgc
    22921 gcacatcgtc gcgctcgcac tcgagcacgg agttaccgag ttattgagct cggatcggga
    22981 cttccgccgg ttctcgaccc tgaacgtgcg cgacccgttc ggctcgagtc gtgcgggcga
    23041 gccacgagga cgctatcggg tgcgtggccg gcggagagcg gcgcacgctc agccgtgacg
    23101 tgcgagcgct ctcgtggtaa ctcccgccct cgatgtcgga acggctggtc ccgctcggta
    23161 agcgcacggc ggaggccccc atgcgcccgc agccggccgc cgacgcgccc aaggtcttcg
    23221 tagagaccta cggctgccag atgaacgtcg ccgacagcga cctgatcggc agcgtgctcg
    23281 ccgatgccgg ctacgcgcaa gcaacacgcg ccgacgaagc cgacgtcatc gtcgtcaata
    23341 cgtgcgcggt ccgcgagaaa gcggaggagc gcgtgatcgc gcgcgcgagc gagctcggcg
    23401 cactcaagcg caaacgcccg ggcaccgtgc tcgcgatcgt cggctgcatg gccgagcacc
    23461 tgaaggaagg gctcgccgag cgcgcgcccg cggtcgacgt gatcgccggt cccgacagct
    23521 atcgccgcat gcccgatctg ctcgccgccg cgcgcgccga acacgcgcat ccgctcgtcg
    23581 acatcaagct cgacaaggcc gagacgtacg aaggcctctc cggcgccatc ggtggcgacg
    23641 gcgtctccgg cttcgtcacg atccagcgcg gctgcgacaa gttctgcacg ttctgtgtcg
    23701 tgccgttcac acgtggccgc gagcgcggtg cgtcgccgcg cgagatcctg cgccaggtgc
    23761 gcgagctcgt cgcggcgggc tatcgcgaga tcacgctgct cggccagacc gtgaactcgt
    23821 acgtctggga agacgtcacg ttcgcgcagc tgctccgcac cgtcgcgagc gtcgacggcc
    23881 tcgagcgcat tcgcttcacg tcgccctacc cggtcgattt caccgacgac gtcgtcgccg
    23941 cgatcgccga ggaatcgaag gtctgcaagt acgtccacct gccggtgcag tccggcagcg
    24001 acgtcgtgct cgagcggatg cggcgcggct acacggtcgc cgactaccgt gcgatcgtcg
    24061 cccggctgcg cgctgcgatg cccgacatcg cgctgtcgac ggacattctc accggcttct
    24121 ccggcgagac cgacgacgat cacgcgcaga cgctcgcact catgcgcgag attcgcttcg
    24181 acagcgcgtt catgttcgcg tactcggaac gcgacctcac gttcgccgcg aagaagctgc
    24241 ccgatgacat cccggccgcg gtgaagcaga agcgcctcgc cgagatcgtc gcgctacagg
    24301 aaacgatttc ggccgaggtg ttcgccgcgc aggtcggcaa gcgcgaacgc gtgctcgtca
    24361 ccgggcccag caaacgcgat ccagcgcagc tgatcggccg aacagacggt ttcaagtccg
    24421 taatcttgcc tgccggggtc ggttccgtgg gcggattcgt cgatgtcacg atcgagcgcg
    24481 caacgatggc gacgctgttc gcacgcgcgt aagatcggca cgtgggtcgg acctgggtcc
    24541 tcgtgttttg cgtggcgtgt gcgcacggcg ccggtgcggg tggcggcggt ggtggtgaag
    24601 cgtcgttcgc gatcacgtat cccgatgcac cgccgaccgg agcggtcgcg aagcacggca
    24661 agcgcttcta cgcaaagccg gaaggccgtt gcttgtacga gaacggtcgc gaggcgcact
    24721 ggacgatcgg tggcgcacgc gtgacgtccg gcgagctgcc gcctggcctg acgatcgaag
    24781 acggccagat cggcggcgtt cccacgcaga ctggcacgta caagtttcac atcgagttct
    24841 cgaatgtcat gtgcgccggc aagccctaca ccgggcaatc cgtagacggc cagatcaccg
    24901 tgcagtgagt catcaacacc cgctcgtcga gctttcgctt gccgcgctcg gtcgcctagc
    24961 caaggtgccg cgcttcggcg agccagctca tggcgaagac gcagagctcg aaaagatctg
    25021 ggtgcgcgcg ccgctgtccg tcgcgatcgg cggcgcagtg tcggcacgca ccgagctgtt
    25081 caattacctg tgcggcaaga aggtgctcga tcccgacggc cgccagggcg ggtgtgcggg
    25141 cctgcgcatt cgccgcggcc gttcgactcg cttcaaagcg acgcgcgacg acggcaccat
    25201 cgaagagcat ctactgccgc cggagcaggc cgacgacgat gcgctgcgca tgcgtgcgga
    25261 agctgccgag gccgaggtca aggaacgcaa gctcgcgctt caacgagtcg agcatgcgtt
    25321 gccgcgagcg gcacgagccc gccctcgcgg cctcatgatc tggctgtggc cgatctggtg
    25381 gctgctcacg cgcaagcatc gccgcgtgct tgccgaccgc cgcttcaccg agctggcgta
    25441 cgaccaggcg tgcgacgcgc gcgaggccgc gaaccgcgag ctcgaggagg ccgagcaacg
    25501 gattcgcgtc cagcgtggcc gcttcttcga gtcgctgcgc gcgctgtcgt cgggaccgcc
    25561 gctcggcagc aacgtgcgcg aggtcgagct cgtgctcggc gagggcccgc tgccggaggg
    25621 tgtcgagctg atcgagctga cgcgatcgtc gcaagcgtcc gagctcgtcg acgcgatctt
    25681 cctcgtcgag aaagatcggc tgcacgcacc acacggcgat gaaggcaccg cgctcgcggt
    25741 cggcaaaatc aacgacgtga tcccgacgct tcccctgatg ctcggcaagg cacgcgcact
    25801 cgtgatcgca cgcaccgcac gcgacgtgat cgagccggcg ctcgagctgc tcgacgacga
    25861 ggtcaacgac accgaggaag gatttcggct gcgcatcgag cggctagaag cgatgcagat
    25921 cctcgatgcc aacgagttca cgcgcacggt gcttgcgaac atcaagccgc agatcgcgca
    25981 gagcatccac atggtgatcg agcacggcgc ggcgcacctc ggcgccgagc tgcagcggct
    26041 cggccacgag tggcagtccg gcatcgcgat gacgcagacc aatgacgagc tcaaggtcgc
    26101 cgtccagcgc atcgaacagt ccgcaccgct cgacgccaaa cgcatcgccg aagaagtgcg
    26161 gctgctcgtc atgggcggtg cagccggcgc ggcgtacgac ctgtatcccg agctcctcgg
    26221 aacgctgaag ccgcacggtc tcgaggagaa tccgccgaag gcagcgccgc cgttgcccgc
    26281 actggagcta ctgccgtcgc tgacgaatgc gtcgcccgcg aagctatcgg gtgcagccgg
    26341 ctggctcggc ggcctgctcc gctcgttcga aagccgccgc gccgacgtca tgcagaaggc
    26401 gcaggcgcgc atggagcatt tgcgcgaggt cgcgaacgcc gagatcctcg acgccgagcc
    26461 aaagctcttc caggcgatcg atcacgcgct caacgcgttc gtcatgaccg cgatcagtcg
    26521 ccaggaagcg tggctcgacg gggcgatgac gctcgagcgc gagaccgtcg cgcgtgaggg
    26581 ccaagagctc gcgccgctcg cccgcgcgcg cgatttgctc aagcgcgaca tcgggaagct
    26641 gctcgccggc atcgcagcgc tcgagaaaga gaacgcaggt ctcgccgccg ccgcggccgc
    26701 gaacccggtt atttcagccg cgtgatgatc tcgcgcgcga cgcgccgcga gccgagcagc
    26761 gagacgtggc cgagatcgtc gtagacgatc cgttccgagc gcggaatctc gagcgcgtcc
    26821 tgcgtcgacg ccggcacgag cgcatcggcg tgcgagaaga tcatcgtcac gcgtgtgtct
    26881 ttcggcggag gcgccgccgc gagccgctgc acgagcgtcg aaccgatcac gagctcgcgc
    26941 gtcggatggc caattccgaa ttccgaaaca tcagtgccga tgtgcggcga tccgagcgtg
    27001 atcagattcg cgaccgcgcc gtcgccaccg agcagctgca cgaagtagcg gccgaccacg
    27061 ccgcccatcg agtgaccgac gatgtcgacc tgcggcgatc cagtgaccgc ctgcacctgc
    27121 tcgacaaacc atgcgagctg acgcgcaccg gccgcgacgc gcccgagcgt ccagtactcg
    27181 aacccgaaga tcggccccag gcccttgcgc gcgagccgat acgccagcgg caagaagttc
    27241 gcccggttca tcgcgtagcc gtggatgaca atgatcgggc gtgggccctg ctgccgcgct
    27301 ccgggcagcg ggaagaaccc cactggccgg accgcagaca cggccatcgc catgccccac
    27361 tcggccagcg ctgtacgcag agatcctacg ttgcgtttct gaaacgcgcg cagagcgtgc
    27421 ggcaccagcg ggtacagccc gctgaacatc agtgcctgcg acacggcggc aaccccaccc
    27481 cgccaagcga ggtggcgaac cgaatggcgc cgttgcattg ccaagtctag ctagacctcc
    27541 atgccaggca catctgcttc gatttcctgc gcgatttcga gtggaatgcc gaggatgccc
    27601 tgcagccgat ccagcatctt gcgttctgca tgggcgaaca cgtggtcaac gactgccatc
    27661 cgacatgcag cccggtagat cccgcggcgc gattccggat tcagattctt catgtacaag
    27721 tcgggcagat caacgcgttg atcgagaaag gtcgtcgcag cc
//
LOCUS       DASUFX010000523         6814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_778293,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000523 DASUFX010000000
VERSION     DASUFX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778293"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..696)
                     /locus_tag="VFV99_20980"
     CDS             complement(112..696)
                     /locus_tag="VFV99_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061861.1"
                     /translation="MRWLVFAIAITSSTVAAAGDGYFVAESLGGAGYRGELGRYDGAP
                     RWQAGFDIRRGDWTVELFGMATIPDLFFIDCYGEECAYAAKPQAGLGGGGVDIKKRWQ
                     LLYLHRWGKPGVYERPGLFASLHGGARWFVGTEALDGYAGPGLGAGASLEGDVWVIGY
                     YLDVGIDIMTLNGPDDTIHGSAPYIALGGKFGWL"
     gene            complement(866..1267)
                     /locus_tag="VFV99_20985"
     CDS             complement(866..1267)
                     /locus_tag="VFV99_20985"
                     /inference="COORDINATES: protein motif:HMM:NF044571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061862.1"
                     /translation="MFTRLVTILVLLFGLGRLGYAIELGAVTIRNSSNSSIGSVDSDG
                     TIRNSSNSSVGKIEKDGTIRDGSNSSIGKVESDGTIRDSSNSSIGKIDSDGTIRNASN
                     SSIGKVDGYSPRLRHLVAAVLFFGGSVSVLR"
     gene            complement(1308..2171)
                     /locus_tag="VFV99_20990"
     CDS             complement(1308..2171)
                     /locus_tag="VFV99_20990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061863.1"
                     /translation="MHRSLGPYQLVRRLGRGGMAEVFLATSFGASGFQRTVAIKTLAP
                     ELVGNADLERALIHEATLAGKLHHRNLVAVLGLGVDDGLYYVVMEYVDGGDLAGAVLP
                     EPLALHVVGELALGLAHLHDARDQRGLPLGIVHRDISPSNVLVSTTGDVKLADFGIAK
                     ATALEDLTAAGTRKGTHAYMSPEQLAGEPVTPASDQFGLAVTLVELITGTRPFTGDTP
                     WATMEAIRAGAADLTGLAPDVDVVVRRALSPEAAARYQSVDDFRLAVAHVRRARTSAG
                     AAELAAWVKQR"
     gene            2192..3535
                     /locus_tag="VFV99_20995"
     CDS             2192..3535
                     /locus_tag="VFV99_20995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061864.1"
                     /translation="MARAATVYSDILDGGTGRVRCQLIVIDGPDRGRACRLGEAEVTV
                     GTDKGADLVLSDDRVSARHTTVRVDGSRFVVRDLGSTNGTWFEGSRVSEVTVPAGSTL
                     LVGRSALRVEPEAQPLDVPPSQARRFGELVGESLAMREVFAVLERVAAADATVLVEGE
                     TGTGKELVARAVHDASPRRRGPFIAVDCGALPEGLLESELFGHVRGAFTGAAQPRAGM
                     LVRADGGTLFLDELGRITPTVQARLLRVLEERVVRPLGGDSERAIDVRIIAASRDDLD
                     AEVAAGRFRADLMYRLAVVRVALPALRTRREDIPLLVRELLRRRGFADEAPSGPGLDR
                     LLAHSWPGNVRELRNVIDRALALAPGAQRFADLPIRVDGGPVAGEGLAVRSDLPYAEA
                     KAVVLHELERRYLADVLAREGGNLSAASRTSGIDRKHLRTLARKHGLVDDGEGDD"
     gene            complement(3538..4476)
                     /locus_tag="VFV99_21000"
     CDS             complement(3538..4476)
                     /locus_tag="VFV99_21000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5061865.1"
                     /translation="MKRIALLAALTVGGCIFVDDSDGPTDPFCGDGHVNTFGGEQCDD
                     GNKVNGDGCSASCAIEGPYCGDGTVDQASEQCDDGNTMSGDGCSAACLYEPVADAFIS
                     ATWQLRNIATNTVAPCPTGFDTAALYNQPIDTAGNPVGTVIIDLFDCSSGAGTSAPLP
                     AGVYQSWVEIADHNNTTVYAKSLSAVVDVTFSDKTLNVQILSDGGYFQLAWVLRGATS
                     NNTLTCAQAGAAGGLEAVGTDVSNASNSVSDIWNCADGFGITGGYEAATYTISVSALN
                     GQDASIGTAPALTNKVINPRNQVTDLGTVTVPITGM"
     gene            complement(4531..5262)
                     /locus_tag="VFV99_21005"
     CDS             complement(4531..5262)
                     /locus_tag="VFV99_21005"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5061866.1"
                     /translation="MLLDAFGLSVPGRRTNNEDAICARPELGLFVVADGMGGYEGGEV
                     ASAIAVESICELVRRTAGDADVTWPYRIDPQRTVAENEIIVATRLANERIAARRTGVL
                     EQMGSTVAVLRVSGELVVIGHVGDSRVYRLRRGELAQLTIDHSLLAQLMAAGTPVDPS
                     TFQWRHVVTRALGASGEPDVQVDRAQPDDVYLLCSDGLSEVLTTDEIAALLDRSAERA
                     CRALVDAAYAAGSRDNISAIVVRVL"
     gene            complement(5359..6678)
                     /locus_tag="VFV99_21010"
     CDS             complement(5359..6678)
                     /locus_tag="VFV99_21010"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX5061867.1"
                     /translation="MAALLVGCGDNNEVTSGVPVSQQSGATATAWFALQLVLVRETPG
                     FSPPVASRAFAYSGITLYESVVNGMRDFKSLGRVLTVMPEMPAIDLDGNYYWPASANA
                     ALAQISRDMFPNASAENLAAIDELEARLAAAYVEKAGRETLDRSAKYGRAVAAAIFKW
                     STTDGGHEGFSRNFPAGYAPPMGAGLWVPTPPMFQPALQPFWGDNRPFLSPDNGQCDF
                     GMPPPFSTQPGSEFYVEAKEVFDAVNNLTPEQKEIALFWADEAMKTATPPGHSIAIAT
                     QLLDADNASLAVAAETYAKLGIAVSDAFISCWKMKFQFNLIRPISYNRLVFDAMWSSF
                     IVTPPFPEYPSGHSAQSGAGSEVMTAMFGEVGFTDHTNDYRGIAPRSFDSFADAANEA
                     AISRLYGGIHYRSAIEDGLTMGACIGDRVNALPFREAVDSDGDFDGD"
BASE COUNT         1103 a         2332 c         2230 g         1149 t
ORIGIN      
        1 gtatcacgcg ctccttcttc tcctcttcgt cgggcagagg ggtcggggga cgcttgtgtc
       61 tcacgttgtg acacagcgtg tatcacgacg tgatacatgt caaggcgcga tctacaacca
      121 gccgaacttg ccgccgagcg cgatgtacgg ggcgctgccg tggatcgtgt cgtccggtcc
      181 attgagcgtc atgatgtcga tcccgacatc gaggtagtaa ccgatcaccc agacgtcgcc
      241 ttcgagtgat gcaccggcgc cgaggcctgg gcctgcgtag ccatcgagtg cctcggtccc
      301 gacgaaccag cgcgcaccgc cgtgaagcga cgcgaacaga cccggccgct cgtagacgcc
      361 gggtttgccc cagcggtgca ggtagagcag ctgccagcgc ttcttgatgt cgacgccacc
      421 gccaccgagt ccggcttgcg gcttcgcggc gtacgcgcac tcctcgccgt aacagtcgat
      481 gaagaacaga tcgggaatcg tcgccatccc gaacagctcg acggtccagt caccgcgccg
      541 gatgtcgaat ccggcctgcc agcgcggtgc accgtcgtaa cggccgagct cgccgcggta
      601 ccccgccccg ccgagactct cggcgacaaa gtagccgtcg cctgcggcgg caaccgtact
      661 gctcgtgatc gcgatcgcaa atacaagcca gcgcatcctc gtgcgtctca cgcgcatcgc
      721 cttcagcgaa gcgtgcgtcg ctagcgcgtg atcgcgcggt gccgcgtcga cgctgctacc
      781 tcgaggcagc gtgcgacgtc gcagcagctc tgcacaccgt cggcaggaca cgaatgtcgc
      841 gatcgcgtcg cgtgggaggc gcggatcagc gcagaacgct gaccgatcca ccgaagaaca
      901 gcaccgctgc gacgaggtgg cgcagtcgcg ggctgtagcc gtcgaccttg ccgatcgagc
      961 tgttcgaggc gttgcggatc gtgccgtcgc tgtcgatctt gccgatcgag ctgttcgacg
     1021 aatcgcggat cgtgccgtcg ctctcgacct tgccgatcga gctattcgag ccgtcgcgaa
     1081 tagtaccgtc cttctcgatc ttgccgaccg agctattcga cgagttgcgg atcgtgccgt
     1141 cgctgtcgac gctgccgatc gagctgttcg acgaattgcg aatcgtgacc gcgccgagct
     1201 cgatcgcata gccgagccga ccgaggccga acagcaggac gaggatcgtg acgagccggg
     1261 tgaacacgag cggactctac cgctattcga tcgcagctgc gcggcgctta acgttgcttc
     1321 acccacgcag cgagctcggc ggcaccggcg ctcgtccgcg cgcgacgcac gtgcgccacc
     1381 gcaagccgaa agtcgtcgac cgattgataa cgcgcggccg cttcgggcga caacgccctg
     1441 cgaacgacga catcgacatc gggcgcgagg ccggtcagat cggcggcgcc ggcgcgaatc
     1501 gcttccatcg tcgcccacgg cgtgtcaccg gtgaacggcc gcgtccccgt gatcagctcg
     1561 acgagggtga ccgcgaggcc gaactgatcg ctcgcgggcg tgaccggctc acctgcgagt
     1621 tgctcgggcg acatatacgc gtgcgtgcct ttgcgagtgc ccgcggccgt gaggtcctcg
     1681 agcgcggtcg ccttcgcgat gccgaagtcg gcgagcttga cgtcgcccgt cgtcgacacg
     1741 aggacgttcg acggactgat atcgcgatgc acgatgccga gcggcaagcc acgctggtcg
     1801 cgtgcgtcgt gcagatgcgc gaggccgagc gcgagctcgc cgacgacgtg cagcgcgagt
     1861 ggctccggca ggaccgcgcc cgccagatcg ccgccgtcga cgtactccat cacgacatag
     1921 tagaggccgt cgtcgacgcc gagcccgagc actgcgacca ggttgcgatg gtgcagcttg
     1981 ccggccagcg tcgcctcgtg gatcagcgcg cgctcgagat ccgcattgcc caccagctcg
     2041 ggggcgagcg tcttgatcgc taccgtgcgc tggaagccac tcgcgccaaa gctcgtcgcc
     2101 aggaacactt cggccatgcc gccgcggcca agccgccgca cgagctggta gggaccgagg
     2161 cttctgtgca cgtcccgtcg tatcatgtgt cgtggcccgc gccgcgaccg tgtacagcga
     2221 catcctcgac gggggtactg gccgcgtgcg ctgtcagctg atcgtcatcg atggcccgga
     2281 ccgcggccgc gcgtgccggc tcggcgaggc cgaggttacc gttggcaccg acaagggcgc
     2341 ggatctcgtg ctgtccgacg atcgtgtgtc ggcacgccac acgacggtgc gcgtcgacgg
     2401 ctcgcgcttc gtcgtccgcg acctcggctc gacgaacggc acgtggttcg aaggttcgcg
     2461 ggtcagcgag gtcaccgtgc ccgccggctc gacactgctg gtcggtcgct ccgcgctgcg
     2521 cgtcgagccc gaagcacagc cgctcgatgt tccgccgagt caggcgcgcc gatttggtga
     2581 gctcgtcggc gagagtctcg caatgcgcga agtgttcgcg gtgctcgaac gcgtcgccgc
     2641 ggccgatgcc accgtgctcg tcgagggcga gaccggcacc ggcaaggagc tggtcgcgcg
     2701 cgccgtccac gatgcgtcgc cgcgccgtcg cggtccgttc atcgcggtcg attgcggcgc
     2761 gttgcccgaa ggcctgctcg agagcgagct gttcggccac gtgcgcggtg cgttcaccgg
     2821 agctgcgcag ccacgagccg gcatgctcgt acgcgccgat ggcggcacgc tgttcctcga
     2881 cgagctcggc cggatcacgc cgaccgtaca agcgcgcctt ctgcgcgtgc tcgaagagcg
     2941 cgtcgtgcgg ccgctcggtg gtgacagcga gcgtgcgatc gacgttcgca ttatcgccgc
     3001 gtcgcgcgac gatctcgatg ccgaggttgc cgccggtcgg ttccgggctg acctcatgta
     3061 ccggctcgcc gtcgtgcgcg tcgcgttgcc ggcgctgcgc acgcggcgcg aggacatccc
     3121 gttgctggtc cgcgagctgt tgcgccgccg gggctttgcc gacgaagcgc cgagcggtcc
     3181 cggcctcgat cgcctgctcg cccatagctg gcccggcaac gtacgcgagc tgcgcaacgt
     3241 gatcgatcgc gcgctcgcac tcgcgcccgg cgcccaacgc ttcgccgatc tgccgatccg
     3301 cgtcgacggg ggcccggtcg cgggtgaagg gctcgcggtg cgcagcgacc ttccgtacgc
     3361 cgaggcaaag gcggtcgtgt tgcacgagct cgagcgccgc tacctcgccg acgtgctcgc
     3421 gcgcgaaggc ggcaacttgt cggctgcctc gcgcacgtcc ggcatcgatc gcaaacacct
     3481 gcgcacgctc gcgcgcaagc atggtctcgt cgacgacggc gaaggcgacg actaacgcta
     3541 catcccggtg atcggaaccg tcaccgtgcc gaggtccgtc acctggttgc gcgggttgat
     3601 gaccttgttc gtcagcgccg gcgccgtgcc gatcgatgca tcctggccgt tcagcgccga
     3661 aaccgagatc gtgtatgtcg cggcctcgta gccacccgtg atgccgaacc catcggcgca
     3721 attccagatg tcgctcacgg aattgctcgc gttcgacacg tctgtgccca ccgcttcgag
     3781 gccacctgcc gcgccagcct gcgcacacgt gagcgtgttg ttcgaggtcg caccgcggag
     3841 gacccatgcg agctggaagt agccaccgtc ggagagaatc tgcacgttca gcgtcttgtc
     3901 gctgaacgtg acatccacga ccgccgagag cgacttcgca tacacggtcg tgttgttgtg
     3961 atccgcgatc tcgacccatg actggtagac ccctgccggc agcggcgcgc tcgtgcctgc
     4021 ccccgacgag caatcgaaca gatcgatgat gacagttccc accgggttgc ccgccgtatc
     4081 gatcggctgg ttgtacagcg cggccgtgtc gaagccagtc ggacatggcg ccaccgtgtt
     4141 tgtcgcgatg tttctcagct gccaggtcgc gctgatgaac gcgtccgcga ccggctcgta
     4201 cagacacgcc gccgaacacc cgtcgccgct catcgtgttg ccgtcgtcgc actgttcgct
     4261 cgcttggtcg accgtgccgt cgccgcaata cggcccttcg atcgcacacg atgccgagca
     4321 gccgtcgccg ttgaccttgt tgccgtcgtc gcactgctcg ccaccgaacg tgttgacgtg
     4381 accgtcaccg cagaacgggt ccgtcgggcc atcgctgtcg tcgacgaaga tgcacccacc
     4441 gacggtgaga gccgcgagca aggcgattcg cttcatcgtg ccagcctatc cgaaacgccg
     4501 tggcagaagc tggctttgtc aggtcgcaca tcacagcaca cgcaccacga tcgccgagat
     4561 gttgtcgcgg ctgcccgcgg cgtacgcggc gtcgacgagc gcacggcacg cacgctctgc
     4621 cgagcgatcc agcagcgctg cgatctcgtc ggtcgtgagc acctccgaca aaccgtcgct
     4681 gcagagcaga tacacgtcgt ccggttgcgc gcgatcgacc tgcacgtctg gctcgccact
     4741 cgcgccgagt gcgcgcgtga cgacatgccg ccactggaac gtgctcggat ccacgggcgt
     4801 gcccgccgcc atcagctgcg cgagcagcga gtgatcgatg gtcagctgcg cgagctcgcc
     4861 gcgccggagc cgatacacgc ggctgtcgcc gacgtgaccg atcacgacga gctcgccgga
     4921 gacgcgcagc acggcaacgg tcgatcccat ctgctccagc acgccggtgc gacgggctgc
     4981 gatccgctcg tttgcgagcc gcgtcgcgac gatgatctcg ttctccgcga ccgtgcgctg
     5041 tggatcgatc cggtacggcc acgtcacgtc cgcatcgccg gcggtgcggc gcacgagctc
     5101 gcagatcgac tcgaccgcga ttgcactcgc gacctcgccg ccttcgtaac cacccatacc
     5161 atcagcaacg acgaacagac cgagctcggg acgcgcgcag atcgcgtcct cgttgttggt
     5221 acgccggccg ggaacactga gaccaaatgc atcgagcaac atgcacgcgc atagagcgcg
     5281 cgccgtgcca catgcatcac agcatcgcga ttgagcagcc tggggcggcg atgccccgat
     5341 cgcggctagc gcctggggtc agtcgccgtc gaagtcgccg tcgctgtcga ccgcctcccg
     5401 gaacggcagc gcgttgaccc ggtcaccgat gcaagcgccc atcgtcaggc cgtcctcgat
     5461 cgccgagcgg tagtggatgc cgccatacag gcgcgagatc gctgcctcgt tcgctgcatc
     5521 ggcaaacgag tcgaaggacc gcggtgcgat gccgcgatag tcgttcgtgt gatcggtaaa
     5581 cccgacctcg ccgaacattg cggtcatcac ctcggagccc gcgcccgact gcgcagagtg
     5641 tcccgacgga tactcgggga acggaggtgt gacgatgaac gacgaccaca tcgcatcgaa
     5701 cacgagccgg ttgtagctga tcggccggat caggttgaac tggaacttca tcttccagca
     5761 cgagatgaac gcgtcggaga cggcgatgcc gagcttcgca tacgtctcgg ccgcgacggc
     5821 gagcgatgcg ttgtccgcgt cgaggagctg cgtcgcgatc gcgatcgagt gccccggtgg
     5881 cgtcgccgtc ttcatcgctt catcggccca gaacagcgcg atctcctttt gctcgggcgt
     5941 caggttgttc accgcatcga agacttcctt tgcctcgacg tagaactccg agcccggttg
     6001 tgtcgagaac ggcggcggca tgccgaaatc gcactggcca ttgtccggcg agaggaacgg
     6061 tcggttgtcg ccccagaacg gctgcagcgc cggctggaac atgggtggcg tcgggaccca
     6121 caagcccgcg cccatcggcg gcgcgtagcc cgccgggaag ttgcgcgaga acccttcgtg
     6181 gccaccgtcg gttgtcgacc atttgaagat cgccgccgcg actgcgcgac cgtacttcgc
     6241 cgagcggtcg agcgtctctc ggccagcctt ttcgacgtac gcggcagcga gccgcgcctc
     6301 gagctcgtcg atcgcggcga ggttctccgc gctcgcgttc ggaaacatgt cgcgcgagat
     6361 ctgtgcgagc gctgcattcg ccgacgccgg ccagtagtag ttgccgtcga gatcgatggc
     6421 cggcatctcg ggcatgaccg tgagcacacg gccgagcgac ttgaagtcgc gcataccgtt
     6481 aacgaccgac tcgtagagtg tgatgccgct gtacgcgaac gcccgcgacg caaccggtgg
     6541 cgagaagcct ggtgtttcgc gcaccaatac gagctgtagc gcgaaccatg cagttgccgt
     6601 cgcgccgctc tgctggctca ccggtacacc gctcgttacc tcgttgttgt cgccgcaacc
     6661 gaccagcagc gcggccatca cgcacaggga ttgtctggct ctcgtcatgg cgagccagcg
     6721 cagttcaagc gtcgtgcccc gatcgtgagg cgctgctagg agcgtgtagc tcatagatcg
     6781 gtcttccgga ctgaacgcac gatccggatc gacg
//
LOCUS       DASUFX010000524         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_82652,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000524 DASUFX010000000
VERSION     DASUFX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82652"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFV99_21015"
     CDS             complement(<1..527)
                     /locus_tag="VFV99_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061868.1"
                     /translation="MRLGTCIALELAVVVSAVGGVYVGVGRTFDLASNYFSAHEAEAS
                     TTFASAQTPSRLPRASLVVAIPEIRAPNNVFGAHDDNLLAPIGAAPVSRVKLNHGGTS
                     LSLRIDFMNGARASFKPEQVWPQSDPRREIAAFRIDRLLGIGHVPPSKPIKFTVAELI
                     AATDPQLRTYMTQRLA"
     gene            complement(839..1867)
                     /locus_tag="VFV99_21020"
     CDS             complement(839..1867)
                     /locus_tag="VFV99_21020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5061869.1"
                     /translation="MTFGERIGAAIVRPSAALATAGDRKHAGRSGSDLLVMMLVLLVA
                     TQLRALTAAIWLGIAVDPTLGVRAVVSTLTDSLVVDLGFLVVGAVIIFAASGRRRELG
                     RAFDFACVAALPLILVALAASLIVYSTGLDVPRPVMWMLSGIAYAWAGGLMALACALA
                     RRPSPAVAGTKLMRRAGWGVLVVALAGIFVQVLWITGHVERVRPMTQGDPAPAFALPT
                     ITAEHQLGPPFTLDSARGHVVVLDFWATWCGPCLRAMPKLDALARAHPDVVVVAINID
                     DPAQAWSIFAERKYAMTLLAGDRETSDRYGVSAIPHTVVIDREGRVRAVFRGGAANLE
                     RQVTALLK"
     gene            complement(1864..2970)
                     /locus_tag="VFV99_21025"
     CDS             complement(1864..2970)
                     /locus_tag="VFV99_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061870.1"
                     /translation="MIEHTPLDVARLSPAAQKALGPGPAKMMAARGVAPLPPIDQVSV
                     LYQISLDADANLAKSARESAAKLPDNILAGVLANPSLDARVIDFFAQHTATRPAVFDA
                     IVQNPSIADETLAFMAGKLGPREVDRIAANEQRMLRHPQIIGAMYMNQAARMSTVDRA
                     VELAVRNNVRVPGLAAWDEIARAIQSEKLQPSAENDALFAMAADASTGDDSALTQGDG
                     DQVIDEDQPQDFTQTQDIPIDKMTMPMKIRLATLGNGFARAILIRDPMRVVAMAAIKS
                     PAVTEFEAGRYARNHSLSDDVIRYIASKREFTKLYGTKYALCRNPKTPIPEAMRLMPF
                     LREKDLTQLGRSKGVPSAVVAQAKKILMQRRGGK"
BASE COUNT          519 a          997 c          994 g          503 t
ORIGIN      
        1 gcgagtcgct gcgtcatgta cgtgcgcagc tgcggatccg tcgcggcgat gagctctgcg
       61 accgtgaact tgatcggctt cgacggcggc acgtgaccga tgccgagcaa gcgatcgatg
      121 cgaaacgccg cgatctcgcg acgcggatcc gattgcggcc acacctgctc gggcttgaac
      181 gacgcgcgcg cgccgttcat gaaatcgatg cgcagcgaca gcgatgtgcc gccgtgattg
      241 agcttgacgc gcgacaccgg cgcggcgccg atcggcgcga gcagattgtc gtcgtgcgcg
      301 ccgaacacgt tgttcggtgc tctgatttcc ggaatcgcga ccacaagcga tgcacgcggc
      361 aagcgcgacg gcgtctgcgc ggacgcgaac gtcgtcgatg cttccgcttc gtgcgcgctg
      421 aaatagttcg acgcgagatc gaacgtgcgg ccgacgccga catagacgcc gccgacggcg
      481 ctcaccacga cggcgagctc cagggcgatg caggtcccta gccgcacacc gaaaatttaa
      541 cacgtgcgcg aattccctcg caaaacgaga gttattcggg cggcgtgtcc cggtccgcag
      601 agcttgcgcc ttcgacgaca tagctcgctt ttcggtgccg cgcggtcggg acgtgcttct
      661 cccagcacgc gaccgagcaa aagcgcagct tcatccgtcc cgtattgcac gacgcgaccg
      721 tgcaacggat cgcgttcgtc ccgggcggta tcagcttgcg gcacgtggcg cagaccaggc
      781 tcgccatgga ccctctgtta gcaggtatgc gcttgcatat ccacttccgg atctgcacct
      841 acttcagtaa cgcagtgact tggcgctcga gattcgctgc accgccgcga aacaccgcgc
      901 gaacgcggcc ctcgcgatcg atcacgaccg tatgtgggat ggccgatacg ccgtaccgat
      961 cgctggtctc gcgatcgccc gccaggagcg tcatcgcgta tttgcgctcc gcgaagatcg
     1021 accaggcctg tgccgggtca tcgatgttga tcgctacgac gacgacgtcc ggatgtgcac
     1081 gcgcgagtgc gtcgagcttc ggcatcgctc tgaggcacgg cccacaccag gtcgcccaga
     1141 aatcgaggac gacgacgtgg ccgcgcgcac tgtcgagcgt gaacggcgga ccgagctggt
     1201 gctcggcggt gatcgtcggt agcgcaaacg caggcgccgg atcgccttgg gtcatcggcc
     1261 gcacgcgctc gacgtggccg gtgatccaca gcacctgcac gaaaatcccg gcaagcgcga
     1321 ccacgagcac gccccaccct gcccggcgca tgagcttggt gcccgcaacc gctggcgaag
     1381 ggcggcgcgc tagggcgcag gcgagcgcca tgaggccacc ggcccacgca tacgcgatcc
     1441 ccgacagcat ccacatcaca ggccgcggca cgtcgaggcc ggtgctgtac acgatcagcg
     1501 acgcggccag cgcgacgagg atcagcggca acgcggcgac acatgcaaaa tcaaacgcgc
     1561 ggccgagctc gcgccttctg ccgctcgccg caaagatgat cacggcgccg acgacgagaa
     1621 acccgaggtc gacgacgagc gagtccgtga gcgtgctgac gaccgcacgc acgccaagcg
     1681 tcggatcgac ggcgatcccc agccagatcg cagcggtcag cgcacgcagc tgagtcgcga
     1741 ccaggagcac cagcatcatg acgagcaagt ccgagcccga gcgcccggca tgcttgcgat
     1801 caccggccgt cgcgagtgca gcgctcggcc gcacgatcgc agccccgatc cgctcgccga
     1861 acgtcacttg ccgccgcggc gctgcatcag gatcttcttt gcctgtgcga ccaccgccga
     1921 cggcacgccc ttcgacctgc cgagctgcgt cagatccttc tcgcggagga acggcatcag
     1981 ccgcatggct tccgggatcg gcgtcttggg gttccggcat agcgcgtact tcgtgccgta
     2041 gagcttcgtg aactcgcgtt tggacgcgat gtagcggatt acgtcgtcag acagcgagtg
     2101 gttacgggcg tagcggccgg cctcgaattc ggtgaccgcc ggtgacttga tagcagccat
     2161 cgcgacgacg cgcatcgggt cgcgaatcag gatggcgcga gcgaacccgt tgccgagcgt
     2221 ggcgagccga atcttcatcg gcatcgtcat cttgtcgatc gggatgtcct gggtctgggt
     2281 gaagtcctgc ggctggtcct cgtcgatgac ctggtcgccg tcgccctgcg tcagcgcgct
     2341 gtcatcaccg gtgctcgcgt cggccgccat cgcgaacagc gcgtcgttct cggcgctcgg
     2401 ctgtagcttc tcgctctgga tcgcgcgggc aatttcgtcc cacgcagcga gcccgggcac
     2461 gcggacgttg ttgcggaccg cgagctcgac cgcgcgatcg accgtcgaca tgcgcgcggc
     2521 ctggttcatg tacatcgcgc cgatgatctg cggatggcgc agcatgcgct gctcgttcgc
     2581 cgcgatgcga tcgacctcgc gcggtccgag cttgcccgcc atgaacgcaa gagtttcgtc
     2641 ggcgatcgac gggttctgca cgatcgcgtc gaacaccgcg gggcgcgtgg ccgtgtgctg
     2701 cgcgaagaaa tcgatcacgc gcgcgtcgag gctcggattc gcgagcacgc cggcgaggat
     2761 gttgtcgggc agcttcgcag cgctctcgcg tgcgctcttg gcgaggttcg cgtcggcatc
     2821 gagcgagatc tgatagagca cgctcacctg atcgatcggc ggcagtggcg cgacgccacg
     2881 cgcagccatc attttcgcag ggccaggtcc aagcgctttt tgcgccgcgg gcgagagccg
     2941 cgcgacgtcg agcggagtgt gttcgatcat gattggttgc gttgatagag aatcttgaga
     3001 cccttgagcg tga
//
LOCUS       DASUFX010000525         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_502083,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000525 DASUFX010000000
VERSION     DASUFX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502083"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFV99_21030"
     CDS             complement(<1..185)
                     /locus_tag="VFV99_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5061871.1"
                     /translation="MTNHAVLIVGGGPTGLMLAGELALARVDVAIVERRLTQDVEGQR
                     AGGLHARAIEILDQRGIA"
     gene            249..698
                     /locus_tag="VFV99_21035"
     CDS             249..698
                     /locus_tag="VFV99_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061872.1"
                     /translation="MERDGSRAPLAGSCHCGALTIELETTRDPSDLPVRLCGCAFCTK
                     HRPRYTSDPAGHVTIRIDDESDVSRYRFGLRLADFLICKVCGVFVAAVEPGPPARAVI
                     NLDVLARAADFTAAPSEFTAYDTEDAAARTARRAKNWTPATLTVTPR"
     gene            complement(737..1072)
                     /locus_tag="VFV99_21040"
     CDS             complement(737..1072)
                     /locus_tag="VFV99_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061873.1"
                     /translation="MPTEVIIRVFAHDHDLRGWLVDELALMSPTIEVQTTAILDAGAA
                     QLLIVGLDALLPAELATLGALLERRGVPVIGIGTAPPLATAFVCVLDAKLTSKQLKRA
                     VRDALAAPQ"
     gene            1190..2113
                     /gene="cysK"
                     /locus_tag="VFV99_21045"
     CDS             1190..2113
                     /gene="cysK"
                     /locus_tag="VFV99_21045"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5061874.1"
                     /translation="MLDLVGRTPMVRLRRIAGATAGIAEIACKLEGHNPAGSVKDRAC
                     LAMVEAAERDGRLKPGDTIVEPTSGNTGIGLALVAAVKGYRLILCMPDDASAERRALL
                     AHYGAEVVLTPGRKLMKGAIERAKEIVAANPRCFMPQQFDNPANPQAHRSTTALEILE
                     DTNRAVDAFVAGVGTGGTITGVGDVLKKEIPTIKIIAVEPATSQVLGGRAAAMHAIQG
                     IGAGFVPGVLDRSLIDEVLACEERTAFDMAGRLAREEGITSGMSGGAAVWAAVEIAKR
                     LGKGKLVVTVIPDTWDRYTSIDKPSSMDFII"
     gene            2121..>2736
                     /locus_tag="VFV99_21050"
     CDS             2121..>2736
                     /locus_tag="VFV99_21050"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX5061875.1"
                     /translation="MASRYSVLYGLLLSLVIAIVSYQLYTAPIPPFSIGTPARHPIDA
                     MLVAIVIGMLIRNTVGVPARFAAGVRYAVVSIMPLGIVLMGARLNFFDVVRTSATALI
                     ISVICVCVALSLTIWLCRRVGISQKLGILIGIGTAICGGTAIAVAAPVIEADDDDTAF
                     GIATVTLFGMVAVFVLPLLGHLFGMSQLEFGVWAGTSVHATPQVV"
BASE COUNT          454 a          871 c          932 g          479 t
ORIGIN      
        1 gcgatgccgc gctgatcgag gatctcgatc gcccgtgcat gcagaccgcc cgcgcgctgt
       61 ccctcgacat cctgcgtgag gcgacgctcg acgatggcga catcgacgcg cgccaatgcc
      121 agctcgcccg ccagcatgag acccgtcggg ccgccgccca cgatcaggac tgcatggttc
      181 gtcatgcgat gaccctgcgc tatcaccccg ggcttgtggc aagccgggtg ctgtggcata
      241 ggttggaagt ggagagggac ggtagtcgcg cgcccctcgc aggttcgtgc cactgcggcg
      301 ctctgacgat cgagctggag acgacgcgcg acccgagcga tctaccggta cgcctgtgcg
      361 gctgcgcgtt ctgcacgaag caccggccgc gctacacgtc cgatccagcg ggtcacgtca
      421 cgatccggat cgacgacgag agcgacgtga gccggtaccg attcggtctc cggctcgctg
      481 attttctgat ctgcaaggta tgcggcgtgt tcgtcgctgc cgtcgagcct gggccgccgg
      541 cccgcgcggt gatcaacctc gacgtcctcg cgcgtgccgc ggacttcacg gcagcgccat
      601 cggagttcac tgcctacgac accgaggatg cagccgcgcg gacggcgcgc cgtgcgaaga
      661 attggacgcc ggccacgctg accgtcacac cacggtgacg accttcgacg cgagcatggc
      721 gccgcgatga cattcgctac tgcggtgcgg cgagagcgtc gcgaaccgca cgcttgagtt
      781 gcttgctcgt caacttggca tcgagcacgc agacgaatgc ggtggcaagc ggcggcgcag
      841 tgccaattcc gatcaccggc acgcctcgac gctcgagcag tgcgccgagt gtcgcgagct
      901 cagccggcag gagcgcgtcg aggccgacga tcaggagctg ggcggcgccg gcatcgagga
      961 tggcggttgt ttgcacctcg atcgttggcg acatcagcgc gagctcgtcg acgagccagc
     1021 cacgaaggtc gtggtcgtgc gcaaagacgc ggatgatcac ctcggtcggc atgcaaacgc
     1081 atgatggcag cccgcgcgcg attgccatcg gatctcacgc tctcgcctcg gtgagcgttc
     1141 actcatggtg agaaacgtgt aaggtcggcg cgtgtcgatt gccgagtcca tgctggatct
     1201 agtcgggcgc acgccaatgg tgcgcttgcg ccgtatcgcc ggtgcgactg cggggattgc
     1261 cgagatcgcg tgcaagctcg agggtcacaa ccctgccggt agcgtgaaag accgcgcgtg
     1321 cctcgcgatg gtcgaggctg ccgagcgcga cggacggctg aagcctggcg acaccatcgt
     1381 cgagccgacc agcgggaata ccggtatcgg cctcgcactc gtggccgcgg tgaaggggta
     1441 ccggctgatc ctttgtatgc cggacgacgc atcggccgag cgacgcgcac ttctcgcgca
     1501 ctacggagct gaggtcgtgc tcacgcccgg gcgcaagctc atgaagggtg cgatcgagcg
     1561 ggccaaggag atcgtggcgg cgaacccgcg ctgcttcatg ccgcagcagt tcgacaatcc
     1621 ggcgaacccg caagcgcatc gctcgaccac tgctctcgag attctcgagg acacgaaccg
     1681 cgcggtcgat gcgttcgtgg ccggtgtcgg tacgggcggc acgattaccg gcgttggcga
     1741 cgtgctcaaa aaggagattc cgacgatcaa gatcatcgcg gtcgagccgg cgacgtcaca
     1801 ggtcctcggt ggtcgcgcgg ccgcgatgca tgcaatccag ggcatcggcg ccgggttcgt
     1861 accgggtgtg ctcgatcgca gcttgatcga cgaggtgctt gcgtgcgaag agcgcacggc
     1921 attcgacatg gcaggtcggc tcgcacgcga ggaaggcatc acgtcgggaa tgtccggagg
     1981 cgcagcagtg tgggctgcgg tcgaaatcgc caagcggctc ggcaagggca agctggtcgt
     2041 gacggtgatc cccgatacgt gggatcgcta cacgagcatc gacaagccga gcagcatgga
     2101 cttcatcatc tgagcgcatc gtggcatctc gctattcggt tctgtacggc ctgctgctct
     2161 cgctggtaat cgcgatcgtc tcgtaccagc tgtacacggc gccgatcccg ccgttctcga
     2221 tcggtacgcc tgcgcgacat ccgatcgacg caatgctcgt cgcgatcgtg atcggcatgc
     2281 tgatccgcaa caccgtcggt gtgccggcgc ggtttgcggc cggcgttcgc tacgcagtgg
     2341 tcagcatcat gccgctcggc atcgtgctca tgggcgcgcg tctcaacttc ttcgacgtcg
     2401 tgcggacgtc ggcgaccgcg ttgatcatca gcgtgatctg cgtgtgcgtc gcgctatcgc
     2461 tgaccatctg gctgtgccgg cgcgtcggca tcagccagaa actcggcatc ttgatcggca
     2521 tcggcaccgc gatttgcggc ggcacggcca tcgcagtcgc agcgccggtg atcgaggctg
     2581 acgacgacga cacggcgttc ggaatcgcga ccgtgacgtt gttcggcatg gtcgccgtgt
     2641 tcgtgctgcc actgctcggc cacttgttcg gcatgagcca gctcgagttc ggcgtgtggg
     2701 ccggcacgtc ggtgcacgca acgccgcagg tcgtcg
//
LOCUS       DASUFX010000526         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1613713, whole genome shotgun sequence.
ACCESSION   DASUFX010000526 DASUFX010000000
VERSION     DASUFX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613713"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFV99_21055"
     CDS             complement(<1..1434)
                     /locus_tag="VFV99_21055"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5061876.1"
                     /translation="MAKKRKKGGKPAAKPAPKPEATVAKPEPQKPEPEQREPQKIEEK
                     KSRGAQRRQAARADRNASATSTLGPAEEPRFWFGFEVPWAKLVLARVVVFALLAIDAL
                     RQIEHAPRYGAGDFNVAQLPLLDALAPGRVLYSVSELVLAYLFIFVACGVLTRYVLPI
                     ATVVYGWLYFSSHLDSYQHHYLVWLILLLACFVPWERPAGATATTPVRSWALRLIFIQ
                     LALLYFWAAVSKMNGAWVDGRTLGSQLSGGIRSLVDATVGIKGASWIVILVELTLAAT
                     IWNKRTWWIAAPLGIALHAGIVWTSLEIGLFAWLMLGLYILVIPDRVWTWVATRQPLQ
                     ATREIAGVIGGWFDGAMQWLWWIIAGAIALLLAGVSRFDHGFAVGGVLIGVLLVASIV
                     LRKRVSIAALASAQLLALVTWTAVDRGTMTASDYFRLWGGSAKRLGDPKTSEYAYRKM
                     IEVAPNEGGGHYQLGKLLLDRKADDEGI"
     gene            complement(1437..1841)
                     /locus_tag="VFV99_21060"
     CDS             complement(1437..1841)
                     /locus_tag="VFV99_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061877.1"
                     /translation="MNPAAHLSALTKTWRGRFILAFLAIQLLLPLHYYVLRHDPHDER
                     FAWRMFSPMRMAQCSPRFSIEKQPVPLTTEFHEAWIEVAKRGRYTVLEGMAAKLCKDH
                     PGKPVELSVDCTYLDRPPAHFGHADMCKEPEL"
     gene            complement(1872..>2250)
                     /locus_tag="VFV99_21065"
     CDS             complement(1872..>2250)
                     /locus_tag="VFV99_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061878.1"
                     /translation="LRRLEARGEIRGGRFVTGFVGEQFALPEALDELRGVRNPGPVAD
                     VSRVAATDPLNLVGVLSPGPRVPAIVGNAVLYVDGQAVASLEGGEVVRRAPIPQGARI
                     DDDLTYHPPPRPITAASQAALPL"
BASE COUNT          392 a          758 c          759 g          341 t
ORIGIN      
        1 gatgccttcg tcgtcggcct tgcggtcgag cagcagcttg ccgagctggt aatggccacc
       61 gccctcgttc ggcgcaacct cgatcatctt gcggtacgcg tactccgacg tcttcggatc
      121 gccgagtcgc ttcgcgctgc cgccccacag tctgaagtaa tcggacgcgg tcatcgtccc
      181 gcgatcgacc gcggtccagg tcacgagcgc gagcagctgg gcgctcgcga gcgccgcgat
      241 cgagacgcgc ttgcgcaaca cgatcgatgc aacgagcaac acgccgatca gcacgccacc
      301 gacggcgaag ccgtgatcga agcggctgac acccgcgagg agcagtgcga tggcgccggc
      361 gatgatccac cacagccact gcatcgcacc gtcgaaccag ccgccgatca cgccagcgat
      421 ctcgcgcgtc gcttgcagcg gttgccgcgt cgcgacccac gtccagacgc ggtcgggtat
      481 gacgaggatg tagagcccga gcatcagcca cgcgaacaga ccgatctcta gcgaggtcca
      541 gacaatgccg gcgtgcaacg cgatgccaag cggcgccgcg atccaccacg tgcgcttgtt
      601 ccagatcgtc gccgcgagtg tgagctcgac gaggatgacg atccacgagg cgcccttgat
      661 gccaaccgtc gcgtcgacga gcgaccggat ccccccgctg agctgactac cgagcgtgcg
      721 accgtcgacc catgcgccgt tcatcttcga gaccgcggcc cagaagtaga gcagcgcgag
      781 ctggatgaag atgaggcgca gcgcccacga ccgcaccggc gtcgtcgccg tggcgccggc
      841 ggggcgctcc cacggcacga agcacgccag cagcaggatg agccatacca agtaatggtg
      901 ctggtaggag tcgagatggc tcgagaagta gagccagccg tagaccacgg tcgcgatcgg
      961 cagcacgtat cgcgtgagca cgccgcacgc gacgaagatg aacaagtacg cgagcacgag
     1021 ctcgctgacg ctgtacagca cgcgaccggg cgcaagcgcg tcgagcagag gtagctgagc
     1081 gacgttgaag tcacccgcac cgtagcgtgg cgcgtgctcg atctggcgga gcgcatcgat
     1141 cgcgagcagt gcgaaaacga cgactcgcgc gagcacgagc ttggcccacg gcacctcgaa
     1201 cccgaaccag aagcgcggct cctcggcggg accgagcgtc gacgtggcgc ttgcgttccg
     1261 atcggcgcgt gcagcttgtc ggcgttgtgc gccgcgcgat ttcttttctt cgatcttttg
     1321 cggctcgcgc tgctcgggct cgggcttctg cggttccggc ttcgcgacgg ttgcctccgg
     1381 cttgggcgct ggcttcgccg cgggcttgcc gcccttcttg cgcttcttcg ccatcgctac
     1441 agctccggct ccttgcacat gtcggcgtgg ccgaaatgcg caggcggacg atcgagatac
     1501 gtgcagtcga ccgagagctc gaccggcttg ccgggatggt ctttgcacag cttcgcggcc
     1561 atgccttcga gcacggtgta gcgcccgcgc ttggcgacct cgatccacgc ttcgtggaac
     1621 tcggtcgtca gcggcaccgg ctgcttctcg atcgagaagc gcggcgagca ttgcgccatg
     1681 cgcatcggcg agaacatgcg ccatgcgaac cgctcgtcgt gagggtcgtg ccgcaagacg
     1741 tagtagtgca gcggcagcag cagctggatc gcgagaaacg ccaggatgaa gcggccgcgc
     1801 cacgtcttgg tgagcgcgct gagatgagcc gccggattca cgtcgccgcg agcttacagc
     1861 ggaagcgcca tctacagcgg caacgcggcc tgcgaggctg cggtgatcgg tcgcggcggc
     1921 gggtgatacg tcagatcgtc gtcgatccgc gcgccctgcg ggatcggcgc acgccgcacg
     1981 acctcgccgc cctcgagcga ggcgaccgcc tggccatcga cgtagagcac cgcgttgccg
     2041 acgatcgccg gcacgcgcgg accgggtgac agcacgccga ccaggttcag cggatcggta
     2101 gcggcgacgc ggctcacgtc ggcaaccgga ccagggttgc ggacgccgcg cagctcgtcg
     2161 agcgcttcgg gaagtgcgaa ttgttcgccg acgaagccgg tgacgaaccg gccgccacgg
     2221 atctcgccgc gcgcttcgag ccggcgcagc
//
LOCUS       DASUFX010000527         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_83636,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000527 DASUFX010000000
VERSION     DASUFX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83636"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1448)
                     /locus_tag="VFV99_21070"
     CDS             complement(186..1448)
                     /locus_tag="VFV99_21070"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX5061879.1"
                     /translation="MRILVLIALAGCLGGTKKMAMAPAGMPQAPMVSKLEQEARLEEP
                     ITNAQVSRRTNSLSSDVAVRHRRLSDDSAPDFDEASPDGANLAVGAAGAGSGAAPPPE
                     PVASPEQKEPEKLVIEAWLDMQTADVSATVDVIRSRVVASGGRMVSENVQGSGKSASS
                     AALEFRVPPAQATAVTTWLGTLGYVTNKRLLSSDVSKTLFDQELALKNLELTMERLQK
                     LASQSGPIADLLTIEKEMTRVRGEIEAVKGEQRWLLNRVELATITVSISRAGEQPYEE
                     IPEARMYPGPQLATLTLIEPGMRQRTRVGAGINLRLQRYATFELAVFPKGDGGDSRAV
                     LATFGSALYSSYLQDGHRRYLNPYLGVRVGFGYLSGNGYPVIAAEAGIELFKHEYLVI
                     DTSVRAIAYVSESADGALNATLGASVPF"
     gene            1684..1929
                     /locus_tag="VFV99_21075"
     CDS             1684..1929
                     /locus_tag="VFV99_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061880.1"
                     /translation="MLEAISLSALADVTGGSKWSEIRAAARPHCPQTVARFPKAPTNR
                     AQAERIGNACLAEMGEWKASMGGRARIQAGIDDAFPK"
     gene            complement(2231..2992)
                     /locus_tag="VFV99_21080"
     CDS             complement(2231..2992)
                     /locus_tag="VFV99_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061881.1"
                     /translation="MVRSGSSSSTGYTIAVERKHSDVLIAGLILVGATTWLRALAQQL
                     FVLDYVTRAVILFVAACGAAQFARRLLPELDFGKTAIAATVSMAIGIGVFAHRIGEPM
                     NIDTLIAIGVALGGAIVGALTSRHRGEPVAKVWLVLGGGFAGFGAAILMIGMTMIVAD
                     GEGAYLLALLGGATGGALLAGLFTPVRGRHAGLGLGIGFAILMGASTSDKSWEIVGVV
                     GLLLGSVLGAIGGGIGARIADRRKSKPHLPEAHVR"
     gene            2964..4040
                     /locus_tag="VFV99_21085"
     CDS             2964..4040
                     /locus_tag="VFV99_21085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061882.1"
                     /translation="MDELDPDRTKVSVVDPLVGSELDRRYRIEFRLAAGGFGAIYRAT
                     HVINRRAVALKVLHTSLASEADVVARFRREAQALGALRNPHTVMAYDFGETADGTLYI
                     AMELLQGESLFERFRALGPLPWRRMLAIVEQVCSSLAEAHAIGIVHRDLKPTNIHLEE
                     VGGDPDYVKVLDFGIAKILHGSTLDSIELTRSGQMIGTFDYMAPEQMFGGAVSPRCDI
                     YTLGVLMYEMISGDKPYGDPESPAAMMQAIVTRKVPPLSTVSDAPAALDAVVMRCLER
                     EAAKRYPSVSALADDIDRVLSDDDTKTDARMSFGDDTATAIDKAPEPRDTVETMDAVD
                     PRTTLPGIAPPERNTPWPLSPRRK"
     gene            4229..5179
                     /locus_tag="VFV99_21090"
     CDS             4229..5179
                     /locus_tag="VFV99_21090"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HEX5061883.1"
                     /translation="MLSRIAFAAFALTAPILSTACTDQVDDELDGDDATEGDASKADA
                     PGSTYTYYFVKPDLRRCASPVCGGVFYRLANAETTRCLDGKKAEQCYAASADWSRLGL
                     GDVGMAKVNDALNKDAFGPGTTLLVRAIVGTKDWGNGLGKFANLRASEAWIAQGPNEA
                     TGPLAKIEDSGVRCIAAPCASLREKKLNSAVSVDIADLGWDKSGARDEQVGEALDRMH
                     TDGLIVAGYRYTVSGPAGTAKARSVTQFYLRATDAAAEKTCFVGGCSGQICSETEGVI
                     STCEWKPEYACYHSAMCEVQADGNCGWTQTAELQACLANP"
     gene            complement(5169..>5941)
                     /locus_tag="VFV99_21095"
     CDS             complement(5169..>5941)
                     /locus_tag="VFV99_21095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5061884.1"
                     /translation="RDWCADDAYETTFLNALSLLFPEGERFFVESVKQQKDRITDPQL
                     QRDIAGFIGQEAMHGREHRAFNDLLVGHGFAEAPRVDARLKWFLKRVRVVLSPMSQLA
                     VTTALEHFTAMMAEALLKDPRMRDRMHADVQPLWLWHALEESEHKAVAFDVYKAAGGG
                     YIRRVAIMLLTTVVFFAVQGLVHARLMATRKILWKPWRWVKGFTNMFIYPGFLSRLTP
                     AYLAYFRPSFHPDDRDTKQLIATWQYTLFGSAGLPVRD"
BASE COUNT         1054 a         1978 c         1938 g          971 t
ORIGIN      
        1 cctgatagcc ctcggtgtcg agcgccgcgc tagcgacgcg tgccaccgcg cgctcgaccg
       61 cggccttcag gcgttcggcg tcgagctcgg gaaactctgc ccgcagcaca cgggtaatga
      121 tgtcgacttg gtcgacaccc atggactggc cccgcatgtt gcgacgttac catcgttcac
      181 gtcacttaga acggtaccga tgcccccagg gtggcgttga gcgcgccatc cgctgactcg
      241 ctgacataag cgattgcgcg gaccgaggtg tcgatgacga ggtactcgtg cttgaacagc
      301 tcgatgccgg cctcggcagc gatcacggga tagccattgc cgctcaaata gccaaacccg
      361 acgcgtacgc cgaggtacgg gttcaagtag cgccggtggc catcttgtag atagctcgaa
      421 tacagcgcgc tgccgaatgt cgcgagcacc gcccgcgaat cgccgccatc gcccttcggg
      481 aacaccgcaa gctcgaacgt cgcgtagcgc tgcaggcgca ggttgatgcc tgcaccgacg
      541 cgcgtgcgct ggcgcatgcc gggctcgatc agcgtgagcg tcgcgagctg tggcccggga
      601 tacatgcgcg cctcgggaat ctcctcatac ggttgctcgc cggcgcgact gatcgagacc
      661 gtgatcgtcg cgagctcgac gcgattgagc agccaacgct gctcgccttt gaccgcttcg
      721 atctcgccgc gcacgcgcgt catctccttc tcgatcgtga gcagatcggc gatggggccg
      781 ctctgcgacg cgagcttctg caggcgctcc atcgtcagct cgaggttctt cagcgcgagc
      841 tcttgatcga atagcgtctt gctgacgtcg ctcgacagca gccgcttgtt cgtgacgtag
      901 ccgagcgtgc cgagccacgt tgtcaccgcc gtggcctgcg ccggcggcac acgaaattcg
      961 agcgccgcgc tcgacgccga tttgcccgag ccctgcacgt tctccgacac catgcggccg
     1021 ccactcgcga cgacgcggct gcgaatcaca tcgaccgtcg cgctgacatc ggcggtctgc
     1081 atgtcgagcc acgcttcgat gacgagcttc tcgggctctt tctgttcggg cgatgccacc
     1141 ggctctggtg gtggcgctgc accggagccc gcgcctgcgg caccgaccgc gaggttggcg
     1201 ccatcggggc tcgcctcgtc aaaatctggc gcgctgtcgt cggagaggcg acggtggcgg
     1261 acggcaacat ccgagctcag cgagttggtt cgtcgcgaca cctgcgcgtt cgtgatcggt
     1321 tcttcgagcc gcgcttcttg ttcgagcttc gacaccatcg gtgcctgtgg catgcccgct
     1381 ggcgccatgg ccatcttctt cgtccctccc aaacaaccag cgagcgcgat caagacaagg
     1441 atgcgcatgc gctcgtgaca cgacgtcgat cgatcggttg cgcggccggc aagcaggttt
     1501 gccactggca gcgtgcgtgg ccgcatcgcg atcgcgcttc tcgcgcaaca aagcaacctt
     1561 gcgcgagggc gacgcagcgc gcgtgacacc tgccgacgac gaatcgtctc ccgcgggaat
     1621 cacggcgcgc gtggcatcgc gactgcactg tgagttgtca caaccttgtc aggagacgca
     1681 gccatgctcg aagcgatttc tctctcggca ctcgccgacg tcacgggtgg atcgaagtgg
     1741 agcgagatcc gcgcggcggc gcggccgcac tgtccgcaga ccgtcgcgcg ctttccgaaa
     1801 gcgccgacca atcgcgcaca ggcggagcgc atcggcaatg cgtgccttgc cgagatgggc
     1861 gagtggaagg cgagcatggg cggccgcgca cggatccagg ccggcatcga cgacgcgttc
     1921 ccgaagtgag cgaggggacc agggcgactt ccccgtttcg gcgccgaaaa aaatgagcgg
     1981 cggcccagga ggaagccgcc gctctaccgg ggtgtccgca atgcgggggc cccgaaacac
     2041 ttgttgatcc accctctgcc gcgcaacgcg cgacggtgac gcagcagcta tagagcaacg
     2101 gccgggcccg ctcccagccc actcaagtgc tcgatcacaa aggcgtgacc tcggctcgac
     2161 cgctagcctg gctactggca tagccggttc aacggacctg agcttatggg agttccggcc
     2221 cttgcgcaga tcaccgcacg tgcgcttcgg gcaagtgcgg tttggattta cgcctatccg
     2281 cgatacgtgc gccgatgccg ccgccgatcg caccgagcac cgagccgagg aggagtccga
     2341 cgacgcccac gatctcccag gatttgtcgc tagtgctcgc gcccatcaga atcgcgaatc
     2401 cgatcccgag ccccagcccc gcatgacggc cgcgtaccgg cgtgaacaag ccagcgagga
     2461 gtgcaccgcc cgtcgcgccg ccgagcaacg cgaggaggta tgcgccctcg ccatcggcca
     2521 cgatcatcgt catgccgatc atgagaatgg ccgcgccgaa gccggcgaac ccgccgccga
     2581 gcacgagcca caccttggcg accggctcgc cacggtggcg cgatgtcagc gcgccgacaa
     2641 ttgcaccgcc gagcgcgacg ccgatcgcga tcagcgtatc gatgttcatc ggttctccga
     2701 tccggtgcgc aaagactccg attccgatcg ccatcgagac agttgccgcg atcgcggtct
     2761 tgccgaaatc gagctcgggc agcaagcggc gcgcgaattg cgccgcgcca cacgcggcga
     2821 caaacaggat cacagcgcgc gtgacgtagt cgagtacgaa caattgctgg gcgagggccc
     2881 tcagccaagt cgtcgcgccg acgagaatca gtccagcgat caggacgtcg ctgtgcttgc
     2941 gctccacggc tatagtgtac cccgtggacg agctcgatcc agaccggacc aaagtgtctg
     3001 tcgtcgatcc gctcgtcggt agcgagctcg atcgccgcta tcgcatcgag tttcggctcg
     3061 cggctggcgg cttcggcgcg atctatcgcg cgacgcatgt gatcaaccgt cgggccgtcg
     3121 cgctcaaggt gctccacacg tcgctcgcga gcgaagcgga cgtcgtcgcg cggtttcgtc
     3181 gcgaagcaca ggcactcggt gcactgcgca atccgcacac ggtgatggcg tacgattttg
     3241 gcgagacggc cgacggcacg ctgtacatcg cgatggagct gctgcagggc gagagcctgt
     3301 tcgagcggtt tcgcgcgctc gggccacttc cgtggcggcg gatgctcgcg atcgtggagc
     3361 aagtctgtag ctcgctcgcc gaggcacacg cgatcggcat cgtgcatcgc gatctgaagc
     3421 cgacaaacat ccacctcgaa gaagtcggcg gcgatcccga ttacgtgaag gtgctcgact
     3481 tcggcatcgc gaagatcctc cacggcagca cgctcgacag catcgagctc acgcgctccg
     3541 gccagatgat cggcacgttc gattacatgg cgccggagca gatgttcggc ggcgcggtgt
     3601 caccacgctg cgatatctac acgctcggcg tgctcatgta cgagatgatc agcggtgaca
     3661 aaccgtacgg cgatccggaa tcgccggcgg cgatgatgca agcgatcgtc acgcgcaagg
     3721 tgccgccgct gtcgaccgtg agcgacgcac ccgccgcgct cgacgcggtt gtcatgcgct
     3781 gtctcgagcg cgaagcggca aagcgctacc cgagtgtctc cgcgctggcc gatgacatcg
     3841 accgcgtgct cagcgacgat gatacgaaga ccgacgcgcg catgtcattc ggcgacgaca
     3901 ccgcgaccgc gatcgacaag gcgcccgagc cgcgggacac cgtcgagacc atggacgcag
     3961 tcgatccgcg cacgacgttg ccggggattg cgccgcccga gcgcaacaca ccgtggccgc
     4021 tgtcgccgcg gcgaaaatag ctgcgacagg tgggacttcg cggtggcgta ctcggcgtct
     4081 cacggcaggc gtactggcca ccgtcgctgg ctaggcgagc tgcaccgtga acctgcatct
     4141 cagcgacgcg tgatcgtttg gaaaatgtaa ctgcgcgacg cgaggttgac gctgcgtggc
     4201 acggaagctg ctcgagggcg cgagcattat gcttagccga attgcttttg ctgcttttgc
     4261 tctgactgcc ccgattctct ccaccgcgtg caccgaccag gtcgacgacg agctcgacgg
     4321 cgatgacgcg acggaaggcg acgcctcgaa ggccgacgct ccgggcagca cgtacacgta
     4381 ttacttcgtc aagccggacc tgcgccggtg cgcgtcgccc gtctgcggcg gcgtgttcta
     4441 tcgcctcgcg aacgcggaga ccacgcgctg cctcgacggc aagaaggccg aacagtgcta
     4501 cgcggcgagc gcggactggt cgcgcctcgg cctcggtgac gtcggcatgg cgaaggtcaa
     4561 cgacgcgctc aacaaggacg cgttcggtcc gggcacgacg ctgctcgttc gcgcgatcgt
     4621 cggcacgaag gactggggca acggcctcgg caagttcgcg aacctccgcg cgagcgaagc
     4681 gtggatcgcg caaggtccga acgaggccac tggcccgctc gcgaagatcg aagacagcgg
     4741 cgtgcgttgc atcgcggcgc cgtgcgcatc gctgcgcgag aagaagctca actcggcggt
     4801 cagcgtcgac atcgcggatc ttggctggga caagtcgggc gcgcgtgacg agcaggtcgg
     4861 cgaggcgctc gaccgcatgc acaccgacgg cctcatcgtc gccggttatc gctacaccgt
     4921 ctccgggccc gcgggcaccg cgaaggcgcg cagcgtgacg cagttctacc tgcgcgcgac
     4981 cgacgccgcg gccgagaaga cctgcttcgt cggcggctgc tcgggtcaga tctgcagcga
     5041 gaccgaaggc gtgatctcga cgtgcgagtg gaagccggag tacgcgtgct accactcggc
     5101 gatgtgcgaa gtgcaggcgg acggcaactg cggttggacg cagaccgccg agctgcaggc
     5161 gtgcctcgct aatccctgac cgggaggccc gcgctgccaa acagcgtgta ctgccacgtc
     5221 gcgatcagct gcttggtgtc gcgatcgtcg ggatgaaagc tcggccggaa gtacgccagg
     5281 tacgccggcg tgagccgtga caggaagcca ggatagatga acatgttggt gaagcccttc
     5341 acccaccgcc acggcttcca caggatcttg cgcgtcgcca tcaggcgcgc gtgcacgagt
     5401 ccctgaacgg cgaagaagac caccgtcgtc agcagcatga tcgcgacgcg acggatgtac
     5461 ccgccgccgg ccgctttgta gacgtcgaat gcgacagcct tgtgctcgct ctcttcgagc
     5521 gcgtgccata gccagagcgg ctggacgtcc gcgtgcatgc gatcgcgcat gcgcggatcc
     5581 ttcagcagcg cctccgccat catcgcggtg aagtgctcga gcgcggtcgt caccgcgagc
     5641 tgcgacatcg gcgacagcac gacgcgcacg cgcttcagga accacttcag cctcgcgtcg
     5701 acgcgcggcg cttcggcgaa gccgtgacca acgagcagat cgttgaacgc gcggtgctcg
     5761 cggccgtgca tcgcttcctg gccgatgaag ccggcgatgt cgcgctgcag ttgcggatcg
     5821 gtgatgcgat ccttctgctg cttcaccgat tcgacaaaga accgctcgcc ttccgggaac
     5881 agcagcgaaa gcgcgttcaa gaacgtcgtc tcgtacgcat cgtcagcgca ccagtcacgc
     5941 g
//
LOCUS       DASUFX010000528        17536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_83984,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000528 DASUFX010000000
VERSION     DASUFX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17536
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83984"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFV99_21100"
     CDS             <1..547
                     /locus_tag="VFV99_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061885.1"
                     /translation="LASADMTCGELDTDPITLVLGARLPAATIATDPLPTSDDSASFP
                     ACSPSATTRCTMATGTNCACDQEGDGQPGATLIARNVPAIDLDEVYVSLRTTFSLHGK
                     VWSSDFVKGKIDATLDTGVLACKLLDGTMCGPNNLRTVRTLNPIVTQQLLTPSTFRSV
                     RVMTGMTCAEIVANQSKLFPR"
     gene            complement(544..1341)
                     /locus_tag="VFV99_21105"
     CDS             complement(544..1341)
                     /locus_tag="VFV99_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061886.1"
                     /translation="MRVEWLACAALLAGCDVLWSIDRVTPIAADGPMLVSCDGGGHNE
                     DGDAYPDDCDVCPGVADDQKDDDEDGVGNACDPSGTETNKLVFFEPFASDTGKWNALN
                     GSWLVRNDKLEYTTVLADNAYVLFNGVVPVPPYVIDYHYTVDQIDPAMASLFMVTIDT
                     TGPSTGGSLGQFRTVGPLKDSIQISFAEPMTGMASDIMTVTAGGYHVHVNYDPGGTVS
                     CRLDADDNTIGGATMLQLSSKPPSGTIGFRSLQVGARVDYIAIYKKS"
     gene            complement(1465..3198)
                     /locus_tag="VFV99_21110"
     CDS             complement(1465..3198)
                     /locus_tag="VFV99_21110"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5061887.1"
                     /translation="MLAIADVSRRYREEKEGRPPALRIRSWRAYWTTWLVIWSYLWLK
                     FRIRFHSDAWVEHKLRQLNLKNARRIERTIIDLQGLFIKVGQLISIMTNFLPEEFRRE
                     LEGLQDSVPPRPYADIETRIREELGKPPTELFASFERRPIASASIGQVHIARLADGSK
                     VAVKVQYPDIEETVRRDLNTLRRIFKIVEWFIPYQGLDELYREIRAIVIQELDYRAEA
                     ENAAKIAANFEGRTDVGFPRVVSELSTSRVLVTHFESGVKITDKAGVKALGLDRGQLA
                     RQVVEIYCQQIFSDGVYHADPHPGNLIVRPSNDSGPPMIVFLDFGAVAEIPANVRAGI
                     VELIQGALTRDTRRIVGAMKQMGFVARGANEQMFEQVIEYFHQRFQENISLDSLNLKD
                     IKFDPQKGLESVADLRRMDISIRELSENFHIPKEIIVLERTLLLLMGLCTELDPTLNP
                     MTVIQPYVERFVLGEEGDWSQLLVETSKDLVMSVTALPAEIRKFMRAAHAGELQLKFK
                     NLEASSQLMYRLGHQVIFVAIGIAGASFAMILEGRGELDRAEWGWWTARISGVLLVWS
                     WWSSRNLLRKR"
     gene            3353..4081
                     /locus_tag="VFV99_21115"
     CDS             3353..4081
                     /locus_tag="VFV99_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061888.1"
                     /translation="MATDDDAAAKLPPAPASEPKEAPERPRAVTSPGPDKSESEKINE
                     RLTERIRHGTIPPEPREPRDAREPRDPREDKLNETIRQRLEHLVPELVKKTFAAGMGA
                     VFSTEEGIRKIAREVSLPDVVGYVASSADGAKDKVFEIVARETRDFLANLNLTEEIAK
                     ILTTLSFEIKTEIRFIPNSERITGAEPDVKASVKLKRNDKAMGSSSDNDDKSDKGSRL
                     RFWRRDGESTDAANDDDESGGHDK"
     gene            4231..5697
                     /locus_tag="VFV99_21120"
     CDS             4231..5697
                     /locus_tag="VFV99_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061889.1"
                     /translation="MRSLGVALLFAATGCAQLFGIDETTGGSDPTRVSLTMQRWSVGA
                     SVSKNPQDMSKEMGTFLVDDGAGGFTKLAAEHSAADELSAALDTGTPPILFSLPDQTP
                     FQRLWATPNRDRRGVFAAFEHANALAPMPNSEIMLSATLPSGYASNESFRIEAVGAWM
                     AYGLTAANLPAPDLVNTAISAMIPFMSFAKMTSFPAAAITSQDVVVFERYVGNQLTGV
                     YQAPPFDQTDGPDPITANVVGVPANKPLTAMIMPSTYSQRFSAVRPAVGALSQSWVVR
                     AAPGWSIGSITGPLLYAGTPAATDTMIMTMFGNPFESLDWRSLLQYVAQENLTYMFMG
                     TLALGLSAQLYVVAEPTGTLNFDMPAGLPINIRANQTPLSTDGMMVMLDPAKPVEVDA
                     ITDKPAATVYFVALYEVTMSADGMAAERHLLVDAVTTGEPKVKFPRDLFQTDHFYYFD
                     FRTMQGGYLQAAMGDLQTLTLPYQVSRADSAVFQVVAQ"
     gene            5694..6713
                     /locus_tag="VFV99_21125"
     CDS             5694..6713
                     /locus_tag="VFV99_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061890.1"
                     /translation="MKLLLACVSIIVLCGVAHADKKEQADALFKQGKKHMEQKHFADA
                     CEAFEKSNKLDPAIGTQLNIAKCYEEWGKIGQAYLAYQAAEKQAAGAKDPREPKIHEL
                     VVALEPNVPHLTIKVPKDAPADLKVTLDSRPVATLGDAFVLDPGPHTIEWWLKGSAKK
                     SKIVPVERGGDSEVTLEMPIVVGGAGDKTGGGDKTGGGDKTGGGDKVAQSTTSKKGKN
                     MRLGGMVLGGAGVVAIGISSYMALSARSDYKDGLAMYCGGMTNNCDMMGLEVTHDARS
                     TANKATVLFIIGAAMVGGGAALYFLAPKGPAQPTAPTETQQTSFYLSPSVSPDGVGVV
                     FGGKL"
     gene            6755..7228
                     /locus_tag="VFV99_21130"
     CDS             6755..7228
                     /locus_tag="VFV99_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061891.1"
                     /translation="MRTLLPIAVVLAMGSPARADGVDPQKVASRWLDAARAGSAKQLA
                     ALTAPSLIVTDVHSQVSTFCIRRMDIGSRARLVRAVRDFKVMFADTAGWQRTEDARDQ
                     PVCPAGRKRGRDTLVAKPGEHDVTFEGGAGVGFVVRVSKAGRVVFVGKSTVEGDH"
     gene            complement(7284..8831)
                     /locus_tag="VFV99_21135"
     CDS             complement(7284..8831)
                     /locus_tag="VFV99_21135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061892.1"
                     /translation="MQGPNPGEILLGKYRVEEIIGVGGMGRVVKASHLYLQQPVAIKI
                     LLPEMAESQSTVARFLREAQSTVRLRSEHIARVMDVGTMPDGCPFMVMEYLEGHDLNQ
                     ILRHHGPQLPQAVVDLILQACEGMAEAHAMGIIHRDIKPSNFFITRRPDGSNLLKILD
                     FGISKTPAEVSELTGTQTVIGTPTYMAPEQMVSARSTDPRSDIWSIGVVMYQLLEGRP
                     PFEAETYAQLVLKVGTAAPTPLHVQLPPGLQDIVFRCLEKDPSRRIQSVGELARMLAP
                     YASDPMSASQAAERASRILTAPKGSQPGFPLAGMQGGLQMTPPQLTPKSWSKTGGSSL
                     SGGAGQMGTKVLNKGRGAVIAGVAALVVCAGIGGFFIAGAMKGDTKAAAKTDDSEPAK
                     HDDIKVNMSAQPVAGGSNVDATSGAAKVDTAAKTDATKTDASKTVKTDATKTDASKTA
                     KTDATKADAAKTDAAKTASKSSTTKTASKTSSKSSSTKTHTTKTTKTEKTEKAEKKDD
                     DLFDDRK"
     gene            complement(8889..9812)
                     /locus_tag="VFV99_21140"
     CDS             complement(8889..9812)
                     /locus_tag="VFV99_21140"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5061893.1"
                     /translation="MAIKKVDSIWMDGSLIPWDSATDHVLAHTMHYGVGVFEGIRAYQ
                     RSDGKSHIFRLREHVERLLDSAAICTMECPYTRDQLMQACIDVVKTNKMGAAYLRPLI
                     YLGYGALGLGSLESPVRTMVACYEWGAYLGDDGLKKGIKCMISGFSRSNGNAVMNKGK
                     ICGQYVTSVLAKRMANKSGFDEALMLDPQGYVAEGTGENIFVVKHGIVRTPPTSAAIL
                     AGITRDTAITLLREQGVEVREEAVARDELYTADEVFLTGTAAEITPVRDIDHRKVGRG
                     EAGPVTRRLQESFFSVVKGSDAKHDHWLSFI"
     gene            complement(9927..12839)
                     /gene="glnE"
                     /locus_tag="VFV99_21145"
     CDS             complement(9927..12839)
                     /gene="glnE"
                     /locus_tag="VFV99_21145"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX5061894.1"
                     /translation="MVRPSDLAVGAPDQADAQRRAERLFDAAAQSGLDLTHLDETGTQ
                     IATLACQRAPYLATLLTRDPLRLGRVAHDPYLRREKPRDQLVAEVTGATSAVTTAAGL
                     RAALRRIRGDELVRLGVRELELGLDTEVGRELSRLADVCFDAAIAFHGAELSARYGQP
                     RYIDDDGNERVALLAVIGMGKLGGEELNFASDVDVIYVYSSDQGEAGSISLHEYFAKL
                     CAQVTGALSEVTEDDLVFRVDLRLRPEGGKGAIAGSLDALERYYETFGRPWERQAWIK
                     ARACAGDRTLGDETIKMLEPFVFPRITSPAVLDDVRELNRRIKRELVRSGDGFDVKNG
                     EGGIREIEFFVQALQLIHGGKRPSIRKRGTLAALDALLFAGLVTDEEHLVLYRAYRWL
                     RHAEHVLQLEAGLQTQTIPADADERARFAARISSPAIDTDLFRHTAAVARLFKTLGDE
                     PDDRPDILAILHGDLTEEAEAAALARLGFTDVTAARAELARARRRPGSPLSPAAIDRS
                     ARIGTLLFSEIAASADPDQALRALGDMIARRGEAWSIWRLLDEQPAIARLLGSILGAS
                     VYLARTLVDTPELIDLLVELGQTAPTRTAAQVKADLVARLATVDASDPEAVWSAVAEV
                     KNGHTLRVGLADFAGALDPLAVCVELTAIAEACLQVALSIVEASMPKVDGVLSVLALG
                     KLGGYELGYAADLDVIFVYQGDDAALEHFTRLSQRLLGALRQRTPRGRLYEVDTRLRP
                     SGTQGLLVSSLAAFRRYHEQDARLWERQALIKLRPIAGDAALGAEVARLAADTVYGAP
                     HDAQQVAEAIGAMRERIERELGGGFHKRPRVKDPNDPAGPGYSAGDLKFDAGGVIDVE
                     FATQYLQLVHGHAHPALRTTGTAPALRAAAALGIAPHDELELLEHGYRFLRGIEHRLR
                     VVHDQPIHRLPESRDELDRLARRSGFPDGATLLEHVERWQREIRRAYLALLSPAPK"
     gene            12904..14013
                     /gene="dnaJ"
                     /locus_tag="VFV99_21150"
     CDS             12904..14013
                     /gene="dnaJ"
                     /locus_tag="VFV99_21150"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5061895.1"
                     /translation="MRDLYEVLGVERGASQADLKKAYRRLAQQYHPDKCGGDKDSEEK
                     FKEAANAYQVLSDDEQRALYDRYGFDGIRRGAGGGGGGPGFSTVEDIFSAFGDLFGDF
                     FGGRSSGKRQPRGADLRVDLELTFAEAVWGTTKEVKVSRAVGCETCSGTGAKVGSKPD
                     VCKTCQGKGQVVHSQGFFMVQTTCPQCRGAGKTIKDPCEDCRGRGAKPETSTLSVTVP
                     AGVDDGQTLRLAGKGEAIAGGTTGHLYVVLHVQGDERFRRDGDDVLTEVPVSFVKAAL
                     GGEIEIYTLEDNCNGATTIDLKPGTQPGDVIVRRGQGIPHVGEHGRGDHVIQFKVEIP
                     KKLSAKQEELLRELAADLGEDNLRAEKRGLFNRKK"
     gene            14204..15082
                     /locus_tag="VFV99_21155"
     CDS             14204..15082
                     /locus_tag="VFV99_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061896.1"
                     /translation="MANRCYLYACEADPSRHDVAPIGLCEHTNDIALAQLIMVMRGAR
                     RVDSLLFANHFAVVADNAGAAERTLAFLAKLGSGKVVERTQFDQALDQMREVVMQTTL
                     DSHLLLEVGEVLDPEDVDELVAGLADLDAQIERAMRGEEEAWLDELRASWQTTVMPWW
                     ANSLYYSFGTQAVTWNRGDVDAMLAVHDRAMTKLVGHPFRYRIGPGVAPHQLGTIVEA
                     IADLAQDVENVPDPRLNGCEIGVHERDVQITFREGTLFVRLSTSGWPSHGVKRRVMHA
                     LGVLPPPHVEYGRATV"
     gene            complement(15083..16357)
                     /locus_tag="VFV99_21160"
     CDS             complement(15083..16357)
                     /locus_tag="VFV99_21160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5061897.1"
                     /translation="MRRVTLAVLGLTLLAPAVASAEGEGDGDYLQLGGFFGPRLFSSG
                     GLLGYNYEEPAHPDLKNMIGFGARIGKPFGLPWLVPELELVFVPTKTTTEMNVNTSVV
                     WLEPRVHARIDLLPQRRMNPFILVGGGSPIAISSARKTFNSGIVGEGYFGAGIRFDSL
                     KGFVMRFDARISAIPSAEDEGLIGGWVNLEGDINFGIELSPGKPRRPQVVEEQKVVEA
                     GPPADKDADGIPDTQDRCPDRAEDQDNFEDADGCPDIDNDGDRVLDIADKCEGQLETI
                     NGYADDDGCPDSVPADVDALKGSIEGLLYAEGETAVRESATKYLQKIAKVMMANPSIR
                     VVLIGHTDDREAKAFATPVEGQPPPDVATIATDLARARAEAVRQAMATQGIPTGRVVV
                     DGVGAEDPVADNSTVKGRLANRRVEIKLYVPK"
     gene            16477..17157
                     /locus_tag="VFV99_21165"
     CDS             16477..17157
                     /locus_tag="VFV99_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5061898.1"
                     /translation="MRILVVEDEPSLRDGIVDLLSGDGHTITAVGDGVAGVEAGLRDP
                     FDLVVLDLMLPRLDGMEVCRRLRAARPGMPILMLTARGSEDDKVRGLIEGADDYVTKP
                     FSARELLARVRVLGRRAPAGESLDEVQVDGAVIDLARMTVMRGDQRVALTPREVGIVR
                     WLYRNKDRVVTRAELLEQVFGQRGDLQTRAVDMAIAVLRKKLEADPQKPTLIVSVKGA
                     GYAWRLAR"
     gene            17139..>17536
                     /locus_tag="VFV99_21170"
     CDS             17139..>17536
                     /locus_tag="VFV99_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061899.1"
                     /translation="METRALIRKSSVSRAWHAAALLSVLLAALLGAWFFSGWHDVRMR
                     QHDVREAPRRAADQRADELARELHGELEALITREVRRPYFHYQNLMHDPKASVGVSVS
                     PSPLARNPEDKLVLGYCQIDAKGKATTPTI"
BASE COUNT         2973 a         5693 c         5867 g         3003 t
ORIGIN      
        1 gctcgcgtcc gcggacatga catgcggcga gctcgacacc gatccgatca cgctcgtgct
       61 cggcgcgcgg ttgcccgcgg cgacgatcgc gaccgatccg ctgccgactt ccgacgacag
      121 cgcgagcttc cccgcctgct caccgtcggc gaccacgcgc tgcacgatgg cgaccggcac
      181 gaactgcgcg tgcgatcagg agggcgatgg ccagcctggc gcgacgctga tcgcgcgcaa
      241 cgtgccagcg atcgatctcg atgaggtcta cgtgtcgctg cgcacgacgt tctcgctcca
      301 cggcaaggtg tggagcagcg acttcgtcaa aggcaagatc gacgcaacgc tcgatactgg
      361 cgtgctcgcg tgcaagctgc tcgacggcac gatgtgcggc ccgaacaacc tacgcaccgt
      421 ccgtacgctc aacccgatcg tcacgcagca gctgctgaca ccgtcgacgt ttcgttcggt
      481 ccgcgtgatg acgggcatga cgtgcgccga gatcgtcgcg aaccagagca agctgttccc
      541 gcgttagctc ttcttgtaga tcgcgatgta gtcgacgcgt gcgccgactt gcagcgagcg
      601 gaagccgatc gtgccactcg gtggcttcga ggacagctga agcatcgtgg cgccgccgat
      661 cgtgttgtcg tcggcgtcga gccggcagct gacggtgccg cctggatcgt aattcacgtg
      721 gacgtggtat ccgcccgccg tgacggtcat gatgtcgctc gccatacccg tcatcggctc
      781 cgcgaacgag atctggatgg agtccttcaa cggaccgacc gtgcggaact ggccgaggct
      841 gccgccggtg ctggggcccg tcgtatcgat cgtcaccatg aacaggctcg ccatcgccgg
      901 atcgatctgg tcgaccgtgt agtggtagtc gatcacgtac ggcggaacgg gcacgacgcc
      961 gttaaagagc acgtacgcgt tgtccgcgag caccgtcgtg tactcgagct tgtcgttgcg
     1021 aacgagccag ctgccgttga gcgcgttcca cttgccggta tcgctcgcga acggctcgaa
     1081 gaacacgagc ttgttcgtct cggtgccgct cggatcacat gcgttgccaa cgccgtcctc
     1141 gtcgtcgtcc ttctgatcgt ccgcgacgcc cgggcagaca tcgcagtcat cgggatacgc
     1201 gtcgccgtct tcgttgtgac cgccgccgtc gcacgagacg agcatcggcc catcggcagc
     1261 gatcggcgtg actcgatcga tgctccacag tacgtcgcag ccggctagca gtgccgcgca
     1321 agcgagccac tccaccctca cgacacgcat cgtggatcgg ttcgctcccg ctgtctgtaa
     1381 gctaggaacc gagcaaagtc aggggtgtgg caaacgcgag cgccgtgcgg actcgccacg
     1441 ttgtctacgt aaatctacgt aggtctatcg cttgcggagc aggttgcgcg acgaccacca
     1501 gctccacacc aagagcacgc ccgagatgcg cgccgtccac cagccccatt cggcgcggtc
     1561 gagctcgccg cggccttcga ggatcatcgc aaacgaggct ccggcgatgc cgatggctac
     1621 gaagatcacc tggtgaccga gccggtacat cagctggctc gatgcttcga ggttcttgaa
     1681 cttcagctgc agctcgccgg cgtgcgcggc gcgcatgaac ttgcggatct cggccggcag
     1741 cgccgtcacc gacatcacga ggtccttcga cgtctcgacc aggagctgcg accagtcgcc
     1801 ctcttcgccg agcacgaagc gctcgacgta cggctggatc acggtcatcg ggttgagcgt
     1861 cggatcgagc tcggtgcaca ggcccatgag cagcagcagc gtgcgctcga ggacgatgat
     1921 ctctttcgga atgtggaagt tctcggacag ctcgcggatc gagatgtcca tgcggcgcag
     1981 atccgcgacg ctctcgaggc ccttttgcgg atcgaacttg atgtctttga ggttgagcga
     2041 gtcgagcgag atgttctcct ggaagcgctg gtggaagtac tcgatcacct gctcgaacat
     2101 ctgctcgttc gctccgcgcg ccacgaagcc catctgcttc atcgcaccga cgatgcgccg
     2161 cgtgtcgcgc gtgagcgcgc cctggatcag ctcgacgata ccggcgcgca cgttcgccgg
     2221 aatctcggcg accgcgccga aatcgagaaa cacgatcatc ggcggaccgc tgtcgttgct
     2281 cggccgcacg atgagatttc cgggatgcgg gtcggcgtga tagacgccgt cgctgaagat
     2341 ctgttggcag tagatctcga cgacctgccg cgcgagctga ccgcgatcga gaccgagcgc
     2401 cttcacgccg gccttgtccg tgatcttcac gcctgactcg aagtgcgtga cgagcacgcg
     2461 cgacgtcgat agctcgctga ccacgcgcgg gaagccgacg tccgtgcgac cttcgaagtt
     2521 cgccgcgatc ttcgccgcgt tctcggcttc ggcgcggtag tcgagctcct ggatcacgat
     2581 cgcgcggatc tcgcgataca gctcgtcgag gccctgatac gggatgaacc actcgacgat
     2641 cttgaagatg cggcgcagcg tgttgaggtc gcggcgcacg gtctcttcaa tatctggata
     2701 ttgaactttg accgcgacct tgctgccatc ggccaggcgc gcgatgtgga cctgaccgat
     2761 cgacgccgac gcgatcggcc ggcgctcgaa cgacgcgaac agctccgtcg gtggcttgcc
     2821 gagctcctcg cggatgcggg tctcgatgtc ggcatacggg cgcggcggca ccgagtcctg
     2881 cagaccttcg agctcgcgac ggaactcctc gggtaggaag ttcgtcatga tcgagatcag
     2941 ctggccgact ttgatgaaca ggccctggag gtcgatgatc gtgcgctcga tgcggcgcgc
     3001 gttcttgagg ttgagctggc ggagcttgtg ctcgacccag gcgtcggaat ggaaccggat
     3061 ccggaacttc agccagagat agctccagat caccagccag gtggtccagt aggcgcgcca
     3121 gctgcggatg cgcagggcag gcggccgacc ctctttctcc tcgcgatatc gccgagaaac
     3181 gtcagcaatc gcgagcatcg aatcctgggg ctgaggcgca gctggatcag gcgagggcgg
     3241 gaccgagcca ccttcgcccg cagaatccct gcgcgccggc tgcaccatgc agtcgcagga
     3301 taccagcgtt cgtctggttg atcatggaac ggatcggggg tacccccagg gtatggccac
     3361 cgacgacgac gcagccgcga aactaccgcc cgcgcctgcg agcgagccca aagaggcacc
     3421 cgagcgtcca cgtgctgtta cgagcccggg tcccgataag tcggagagcg agaagatcaa
     3481 cgagcggctc accgagcgca tccggcacgg cacgattccg ccggagcccc gagagccgcg
     3541 cgacgcgcgc gagcctcgcg acccacgcga ggacaagctc aacgagacca tccgccagcg
     3601 gctcgagcat ctcgttcccg agctcgtgaa gaagacgttc gccgccggca tgggcgcggt
     3661 gttctccacc gaggaaggca ttcgcaagat cgcgcgcgag gtcagcctgc ccgacgtggt
     3721 cggttacgtc gcgtcgtcgg ccgatggagc gaaggacaag gtgttcgaga tcgtcgcgcg
     3781 cgagacgcgg gacttcctcg caaatctcaa cctcaccgaa gagatcgcga agatcctcac
     3841 gacactgtcg ttcgagatca aaaccgagat aaggttcatt ccgaatagcg agcgcatcac
     3901 cggcgccgag cccgacgtca aagcgtccgt gaagctcaag cgcaatgaca aagcgatggg
     3961 gtcgtcttcg gacaatgacg acaagagcga caagggctcg cggttgcggt tctggcggcg
     4021 cgacggcgaa tcgacggacg ccgcgaacga cgacgacgag agcggcgggc acgacaagta
     4081 aatcgccgta gccaagtgtt tgcgcccccg tgggtatccg gcgttctcgt cggctgatct
     4141 tcagatccgt gatgttccct acgcggtggg atacgtcgat gccggcgtgt tcgaggtggc
     4201 ggcaccgtga cgatgttacg attgaccccg atgaggtcct tgggtgtcgc gctgctgttc
     4261 gcggcgactg gctgcgcgca gctattcgga atcgacgaga ccaccggcgg cagcgacccg
     4321 acgcgggtgt cgctgacgat gcagcgctgg tcggtcggcg cgtcggtctc gaagaacccg
     4381 caggacatga gcaaggagat ggggacgttt ctcgtcgacg atggcgccgg cggtttcacg
     4441 aagctcgcag ccgagcacag tgctgccgac gagctgagcg cggcgctcga taccggcacg
     4501 ccgccgatcc tgttctcgct gcccgaccag acgccgtttc aacgcctgtg ggcgacaccc
     4561 aaccgcgacc gcagaggcgt gttcgcggcg ttcgagcatg cgaacgcgct cgcgccgatg
     4621 ccgaactcgg agatcatgct cagcgcgacg ctaccgagcg gttacgcgag taacgagtcg
     4681 ttccgcatcg aggcggtcgg cgcgtggatg gcctatggcc tgacggccgc taacctgccg
     4741 gcacccgacc tcgtcaacac tgcgatcagc gcaatgattc cgttcatgtc gttcgcgaaa
     4801 atgacgagct tcccggccgc ggcgatcacg agccaggacg tcgtggtgtt cgagcgctac
     4861 gtcggcaacc agctgaccgg cgtctatcaa gcgccgccat tcgatcagac cgatggtccc
     4921 gatccgatca ccgcgaacgt cgtcggcgtc cctgcgaaca aaccgctgac tgccatgatc
     4981 atgccgagca cgtactcgca acgattctcc gcggtgcgtc ctgcggtcgg cgctcttagc
     5041 cagtcgtggg tcgtgagggc ggcgcccggg tggtcgatcg gttcgataac cggcccgctc
     5101 ctgtacgccg gcaccccggc ggcgaccgac acgatgatca tgacgatgtt cggtaacccg
     5161 ttcgagtcgc tcgattggcg ctcgctcctg cagtacgtag cgcaagagaa cctcacgtac
     5221 atgttcatgg gcacgctggc gctcgggctg tccgcgcagc tctacgtcgt cgccgagccc
     5281 acgggtacgc tgaacttcga catgccggcc ggcttgccga tcaacattcg tgcgaatcag
     5341 acgccgctct cgacggacgg catgatggtg atgctcgatc ccgcgaagcc cgtcgaagtc
     5401 gacgcgatca ccgacaagcc ggcagcgacg gtctacttcg tcgcgctcta cgaggtcacg
     5461 atgagcgccg acggcatggc cgccgagcgc cacctgctcg tcgatgcggt aacgaccggc
     5521 gagccaaagg tgaagttccc gcgcgatctg tttcagaccg atcacttcta ttacttcgac
     5581 ttccgcacga tgcagggcgg atatctgcag gcggcgatgg gcgacttgca aacgctcacg
     5641 ttgccgtacc aagtcagccg cgcggacagc gcggtcttcc aggtggtggc gcaatgaagc
     5701 tcttgctcgc atgcgtatcg atcatcgtgc tctgcggcgt cgcgcacgcc gacaagaagg
     5761 aacaggccga cgcgctgttc aagcagggca agaagcacat ggagcagaag cacttcgcgg
     5821 atgcgtgcga ggcattcgag aagagcaaca agctcgatcc ggcgatcggc acgcagctca
     5881 acatcgccaa gtgctacgag gagtggggca agatcggcca ggcctacctc gcgtatcaag
     5941 ctgccgagaa acaggccgcg ggcgcaaaag atccgcgcga accgaagatc cacgagctcg
     6001 tcgtcgcgct cgagccgaac gttccgcacc tgacgatcaa ggtccccaag gacgcgcctg
     6061 cagatctcaa ggtcacgctc gacagccggc cggtagcaac actcggcgat gcgttcgtcc
     6121 tcgatccggg cccgcacacg atcgagtggt ggctcaaagg cagcgcgaaa aagagcaaga
     6181 tcgttcccgt cgagcgcggc ggcgactccg aggtcacgct cgaaatgccg atcgtcgtcg
     6241 gtggtgccgg cgacaagacc ggcggcggtg acaagacggg cggcggtgac aagaccggcg
     6301 gcggtgacaa ggtcgcccaa tcgacgacgt cgaagaaggg caagaacatg cggctcggtg
     6361 gcatggtgct tggcggtgcc ggcgtcgtcg cgatcggtat ctcgagctac atggcactgt
     6421 cggcacgtag cgactacaag gacgggctcg ccatgtactg cggtggcatg acgaacaact
     6481 gcgacatgat gggcctcgag gtgacgcacg acgcgcgcag cacggcgaac aaggcgacgg
     6541 tcctgttcat catcggcgcc gcgatggtcg gcggtggcgc ggcgctgtac ttcctcgcac
     6601 cgaaggggcc ggcacagccg accgcaccca ccgaaacgca gcagacgtcg ttctacctct
     6661 cgccgtcggt gagcccggac ggcgtcggtg tcgtgttcgg cggcaaactg taagtttcgt
     6721 agatcgcgcg gcgcccgcgt gcgttgacgc gaggatgcgg acgctcctac cgattgcagt
     6781 cgtcctcgcc atgggctcgc cggcgcgcgc agacggcgtc gatccgcaga aggtcgcgtc
     6841 gcggtggctc gatgccgcgc gggccggcag tgcgaagcag ctggcagcgc tcacggcgcc
     6901 gtcgctgatc gtgacagacg tgcattccca ggtctcgacg ttctgcatcc gtaggatgga
     6961 tatcgggtcg cgcgcgcgcc tcgttcgtgc cgtgcgtgac ttcaaagtga tgttcgccga
     7021 cacggcgggc tggcagcgca ccgaggacgc acgggatcaa ccggtgtgcc ctgcgggtcg
     7081 caagcgcggg cgggacacgc tggttgccaa gcccggcgag catgacgtga cgttcgaggg
     7141 tggcgccggc gtcgggttcg ttgtccgcgt atcgaaggcg ggcagagtcg tgttcgtcgg
     7201 caagtcgacg gtcgaaggcg atcactagcg attgcgcgca aagcttcggg ggctagtcgc
     7261 cgtgcgtgcg gtccgcggac gcgctacttg cggtcgtcga agaggtcgtc gtccttcttc
     7321 tcggccttct cggtcttctc ggtctttgtc gtcttcgtcg tgtgcgtctt ggtcgacgag
     7381 ctcttcgatg acgtcttgct cgccgtcttg gtggtcgacg acttgctcgc agtcttggct
     7441 gcgtcggtct tcgccgcgtc ggccttcgtc gcgtcggtct tcgccgtctt gctcgcgtcg
     7501 gtcttcgtcg cgtcggtctt caccgtcttg ctcgcgtcgg tcttcgtcgc gtcggtcttc
     7561 gccgccgtgt ccaccttcgc ggcgccgctc gtcgcgtcga cattcgagcc gccggcaacg
     7621 ggttgcgccg acatgttcac cttgatgtcg tcatgcttcg caggctcgga gtcgtcggtc
     7681 ttcgccgcgg ccttcgtgtc gcccttcatc gcgcctgcga tgaagaagcc gccgatgccg
     7741 gcgcacacga cgagtgcggc gacgcccgcg atcacggcgc cgcggccctt gttcagcacc
     7801 ttcgtgccca tctggccagc gccgcccgac agcgagctgc cgccggtctt gctccagctc
     7861 ttcggcgtga gctggggtgg cgtcatctgc aggccgcctt gcatgcctgc gagtgggaag
     7921 ccgggctggc tgcccttcgg tgcggtgaga atgcgcgagg cgcgctcggc tgcttgcgat
     7981 gcgctcatcg gatccgacgc atacggcgcg agcatgcgcg cgagctcgcc gaccgactgg
     8041 atgcggcgac ttggatcctt ctcgaggcag cggaacacga tgtcttgcag ccccggtggc
     8101 agctggacat ggagcggcgt gggcgctgca gtgccgacct tgagcacgag ctgcgcgtag
     8161 gtctcggctt cgaacggcgg gcggccttcg agcagctggt acatgacgac gccgatcgac
     8221 cagatgtcac tgcgcggatc cgtcgagcgc gcggacacca tctgctcggg tgccatgtac
     8281 gtcggcgtgc cgatcacggt ctgcgtgccg gtcagctcgc tgacttcggc aggcgtcttc
     8341 gagatgccga agtcgagaat cttgagcaga ttcgatccgt cgggccgccg tgtgatgaag
     8401 aagttcgacg gcttgatgtc gcgatggatg atgcccatcg cgtgcgcctc ggccatgcct
     8461 tcgcacgcct gcaggatcaa atcgacgact gcctgcggga gctgcggtcc gtgatggcgc
     8521 aggatctggt tgaggtcgtg gccctccaga tactccatga ccatgaacgg gcagccgtcg
     8581 ggcatcgtgc cgacgtccat cacgcgcgcg atgtgctcgc tgcgcagacg gaccgtggat
     8641 tgtgcttcgc gcaggaagcg cgcgaccgtc gactggctct cggccatctc cggcaacagg
     8701 attttgatcg cgacgggctg ctgcaggtac aggtggctcg ccttgacgac gcgccccata
     8761 ccaccgacac cgatgatctc ttctactcgg tacttaccga gcagaatctc cccgggattc
     8821 ggcccttgca cgacgacgca attgtagcag cctttgactc tgctaccgcc aatccgaata
     8881 cctactggct agatgaaact tagccagtgg tcgtgctttg cgtcgctacc tttgactacc
     8941 gagaagaacg attcctggag gcggcgcgtc accgggcctg cctcgccgcg accgaccttg
     9001 cgatgatcga tgtcgcggac cggagtgatc tcggcggcgg tgccggtgag gaacacctcg
     9061 tcggccgtgt acagctcgtc ccgggcgacg gcctcttcgc gaacctcgac gccctgctcg
     9121 cggagcagcg tgatcgcggt atcgcgcgtg atgccagcga ggatcgcagc tgacgttggc
     9181 ggcgtgcgca cgatgccgtg cttgacgacg aagatgttct cgccggtgcc ttcggctacg
     9241 tagccttgcg ggtcgagcat cagcgcttca tcgaagccgc tcttgttcgc catccgcttc
     9301 gcgagcacgc tcgtgacgta ctggccgcag atcttgccct tgttcatgac ggcgttgccg
     9361 ttcgagcgcg agaagccgga gatcatgcac ttgatcccct tcttgagacc gtcgtcgccg
     9421 aggtacgcgc cccactcgta gcaggcgacc atcgtgcgca ctggtgactc gagcgagcca
     9481 agaccgagcg cgccgtagcc gagatagatg agcgggcgca gataggccgc gcccatcttg
     9541 ttcgtcttca cgacgtcgat gcacgcctgc atcagctgat cgcgcgtgta cgggcactcc
     9601 atcgtgcaga tcgccgcgga gtcgagcagg cgctcgacgt gctcgcgcaa ccggaagatg
     9661 tgcgacttgc catcgctacg ctggtacgcg cgaatgcctt cgaacacacc aacgccgtag
     9721 tgcatcgtgt gagcgagcac gtgatcggtc gcggagtccc acgggatgag cgaaccgtcc
     9781 atccaaatgg agtcgacctt cttgatggcc atagggtttc gtatcgcacg ccgaggagag
     9841 atcacaaggc acagtgcatc aaggatcttc tgggcacagg cttaacgcgg cgcggctgtg
     9901 taaaccgttg cgcggccggc aggaatctac ttcggtgccg gtgagagcag cgcgagataa
     9961 gcgcgacgta tctcacgttg ccagcgctcg acgtgctcga ggagcgtcgc accgtcggga
    10021 aacccggacc gccgcgcgag gcgatcgagc tcgtcgcgcg attcgggtaa gcggtgaatt
    10081 ggctggtcgt ggaccacccg caagcggtgt tcgatgccgc gcaggaagcg atagccgtgc
    10141 tcgagcagct cgagctcgtc atggggagcg atccccaggg cagcggccgc acgcagcgcc
    10201 ggtgcggtgc cggtcgtgcg cagcgccgga tgcgcgtggc cgtgcacgag ctggagatac
    10261 tgcgtcgcga actcgacgtc gatcacgccg ccggcgtcga acttgaggtc gcccgccgaa
    10321 taccccggcc cagccgggtc attcggatct ttgacacgcg gccgcttgtg aaaaccgccg
    10381 ccgagctcgc gctcgatccg ttcgcgcatc gcgccgatcg cttcagcgac ctgctgcgca
    10441 tcgtgcgggg cgccgtacac ggtgtcggcg gcgaggcgcg cgacctcggc gccgagcgcg
    10501 gcatcgccgg cgatcgggcg gagcttgatc agtgcctggc gctcccacag ccgcgcgtct
    10561 tgctcgtgat agcgacggaa cgccgcgagc gacgacacga gcaggccctg cgtgcccgac
    10621 ggtcgcaagc gcgtatcgac ctcgtagagc cggccgcgcg gggtgcgctg acgaagcgcg
    10681 ccgagcaggc gctgcgagag ccgcgtgaaa tgctcgaggg cggcatcgtc gccttgatag
    10741 acgaagatga catcgaggtc ggccgcataa ccgagctcgt agccaccgag cttgccgagc
    10801 gcgagcaccg acaagacgcc gtcgaccttc ggcatgctcg cctcgacgat cgacagcgcg
    10861 acttgcaagc acgcctcggc gatcgcggtc agctcgacgc acaccgcgag cggatcgagc
    10921 gcacccgcga agtcggcgag gccgacgcgc agcgtgtggc cgttcttcac ctcggcgacc
    10981 gcgctccaca ccgcttcggg atcgctcgcg tcgacggtgg cgagtcgtgc cacgagatcg
    11041 gctttgactt gcgcggccgt gcgcgtcggc gccgtctggc caagctcgac caacagatcg
    11101 atcagctctg gcgtatcgac gagcgtgcgc gcgaggtaca cgctggcgcc aaggatcgag
    11161 ccaagcaggc gcgcgatcgc cggttgctcg tcgagcaagc gccagatcga ccacgcttcg
    11221 ccgcggcggg cgatcatgtc gccgagcgcg cgcagcgctt gatccggatc cgccgatgcc
    11281 gcgatctcgg agaacagcag tgtaccgatc cgggccgagc gatcgatcgc ggccggcgat
    11341 agcggcgagc cagggcgtcg gcgtgcgcgt gcaagctcgg cgcgcgccgc agtgacgtcg
    11401 gtgaagccga ggcgtgcgag cgccgccgct tccgcttctt cggtcaggtc gccgtgcagg
    11461 atcgcgagga tgtcggggcg atcgtcgggt tcgtcgccga gcgtcttgaa cagccgcgcg
    11521 accgcggcgg tgtggcggaa caggtcggtg tcgatcgcgg gactgctgat ccgcgccgcg
    11581 aaccgcgcgc gctcgtcggc atcggcggga atcgtctgtg tctgcagacc ggcctcgagc
    11641 tgcaggacgt gctcggcgtg gcgaagccag cggtacgcgc ggtacagcac gaggtgttcc
    11701 tcgtcggtga cgaggcccgc gaacaacagc gcgtcgagcg cggcgagcgt gccgcgcttg
    11761 cggatgctcg gccgcttgcc gccatggatc agctgcagcg cctgcacgaa gaactcgatc
    11821 tcgcggatgc caccctcgcc gttcttgacg tcgaagccat ccccgctgcg cacgagctcg
    11881 cgcttgatcc ggcggttgag ctcgcgcacg tcgtcgagca ctgcaggcga cgtgatgcgc
    11941 gggaacacga acggctcgag catcttgatc gtctcgtccc cgagcgtgcg atcgccagcg
    12001 cacgcgcgcg ccttgatcca cgcctggcgt tcccacggcc ggccgaacgt ctcgtagtag
    12061 cgctcgagcg cgtcgagcga gcccgcgatc gcgcccttgc cgccctcggg ccgcagccgc
    12121 agatcgacgc gaaacacgag atcgtcctcg gtgacttcgg agagcgcacc ggtgacctgc
    12181 gcgcagagct tcgcgaagta ttcgtgcagc gagatcgatc cggcctcgcc ctgatccgac
    12241 gagtagacgt agatgacgtc gacgtcggat gcgaagttca gctcctcacc gccgagcttg
    12301 cccatgccga tcacggcaag cagcgcgacg cgctcgttgc cgtcgtcgtc gatgtagcga
    12361 ggttgcccgt accgcgcgct gagctcggcg ccgtggaatg cgatcgcggc gtcgaagcac
    12421 acgtcggcga ggcgggacag ctcgcggccg acctcggtat ccagcccgag ctcgagctcg
    12481 cgcacgccga ggcgcaccag ctcgtcaccg cggatccggc gcagcgcagc ccggagcccg
    12541 gctgcggtcg tgacggcaga tgttgcgccc gtcacctcgg caaccagctg gtcccggggt
    12601 ttctcgcggc ggaggtaggg gtcatgggcg actctgccca atcggagggg atcgcgggtc
    12661 aggagcgtgg ccaggtaggg ggctcgctgg caggccaggg tcgcgatttg cgtgccggtc
    12721 tcgtccaagt gggtgagatc caggccgctt tgcgccgcag cgtcgaacag gcgctcggcc
    12781 cgacgttgcg catctgcctg atcgggcgca cctactgcca gatcagaagg cctgaccatg
    12841 acgggaccat accttgacag ctcgcgctct ccgcattacc caaggcgttg attctcttcg
    12901 gtcatgcgtg atctttatga agtactcggg gtcgagcggg gcgcgagcca agccgatctg
    12961 aagaaggcct accggcgcct cgcgcagcaa tatcaccctg acaagtgcgg cggtgacaaa
    13021 gactccgagg agaagttcaa ggaagccgcg aacgcgtacc aggtgctctc cgacgacgag
    13081 cagcgcgcgc tctacgatcg ctacggcttc gacggcatcc ggcgcggtgc aggcggcggt
    13141 ggcggcggcc cgggcttctc gacggtcgaa gacatcttct cggcattcgg cgatctgttc
    13201 ggtgatttct tcggcggccg ctcgagcggc aagcgccagc cgcgcggcgc ggatctgcgc
    13261 gtcgacctcg agctgacgtt tgccgaggcg gtgtggggca ccacgaagga agtgaaggtg
    13321 tcgcgcgccg tcggatgcga gacgtgcagc ggcaccggcg cgaaggtcgg cagcaagccc
    13381 gacgtctgca agacctgcca gggcaaaggg caggtcgtgc actcgcaggg cttcttcatg
    13441 gtgcagacca cgtgtccgca gtgccgcggc gctggcaaga cgatcaagga tccgtgcgag
    13501 gactgccgcg gacgcggcgc caagcccgag acgtccacgc tgtcggtcac cgtgccggcg
    13561 ggcgtcgacg acggccagac gctgcggctc gccggcaaag gcgaagcgat cgcaggtggc
    13621 accaccggtc atctctatgt cgtgctgcac gtgcagggcg acgagcgctt ccgccgcgac
    13681 ggcgacgacg tgctcaccga ggtgccagtc agcttcgtca aagcagcact cggcggcgag
    13741 atcgagatct acacgctcga ggacaactgc aacggcgcaa ccacgatcga tctcaagcca
    13801 ggcacacagc cgggcgatgt gatcgtgcgg cgcgggcagg gcattccgca cgtcggcgag
    13861 cacggccgcg gcgaccacgt catccagttc aaggtcgaga tcccgaagaa gctatccgct
    13921 aaacaagagg agcttctgcg agagttggca gccgacctgg gtgaagacaa cttgcgcgcc
    13981 gaaaagcgcg gcttgttcaa tcgcaagaag tgacacccac gaagcggcct ctgtcgcttc
    14041 tggcacctcg tggtcgcagc tcaattcaca gcgtcgtagc gcggcttacg cgatgtcggg
    14101 tgtagccaac agatcgttgc acccccaagc atcaactgtg tcagccgcgg caatccgaat
    14161 tgttacgtcc gcgcgcgcgg caccgtgata gctgacgctc gcgatggcga accgttgtta
    14221 cctgtacgcg tgcgaggccg acccgagccg gcacgatgtg gccccgatcg gcctctgcga
    14281 gcacaccaat gacatcgcgc tcgcgcagct gatcatggtg atgcgcggcg cacgccgggt
    14341 cgactcgctc ttgttcgcca atcactttgc cgtcgtcgcc gacaacgccg gcgctgccga
    14401 gcgcacgctg gcgttcctcg ccaagctcgg ctccggcaag gtcgtcgagc gcacgcagtt
    14461 cgatcaagcg ctcgaccaga tgcgcgaagt ggtgatgcag accacgctcg acagtcacct
    14521 gctgctcgaa gtcggcgagg tgctcgatcc ggaggatgtg gacgagctcg tcgccggcct
    14581 tgccgatctc gacgcccaga tcgagcgcgc gatgcgcggc gaagaagaag cgtggctcga
    14641 cgagctgcgc gcgtcgtggc agacgacggt gatgccgtgg tgggcgaact cgctctacta
    14701 ctcgttcggc actcaggcgg tgacgtggaa ccgcggcgac gtcgacgcga tgcttgcagt
    14761 gcacgatcgc gcgatgacga agctcgtcgg ccatccgttc cgctatcgca tcggtccggg
    14821 cgtcgcgccg catcagctcg gcacgatcgt cgaggcgatc gccgatctcg cgcaggatgt
    14881 cgagaacgtt ccggatccgc gcctgaacgg ttgcgagatc ggcgtgcacg agcgcgacgt
    14941 gcagatcaca ttccgtgaag gcacgctgtt cgttcgcctc agcaccagcg ggtggccgag
    15001 ccacggcgtg aagcgccgcg taatgcacgc actcggcgtg ttgccgccgc cgcacgtcga
    15061 gtacggccgc gccaccgtct gatcacttcg gaacgtagag cttgatctcg acgcgccggt
    15121 tcgcgagccg gcccttcacc gtcgagttgt cggcgaccgg atcctcggca ccgacgccat
    15181 cgacgacaac gcggccggtc ggaatgccct gcgtggccat cgcctggcgc acggcttcgg
    15241 cgcgcgcacg cgcgaggtcg gtcgcgatcg tcgcgacgtc gggcggcggc tgaccttcga
    15301 caggcgtcgc gaacgccttc gcctcgcgat cgtcggtgtg accgatgagc acgacgcgga
    15361 tcgacgggtt cgccatcatg accttcgcga tcttctgcag gtacttcgtc gcgctttcgc
    15421 gcacggcggt ctcgccctcg gcgtagagca ggccttcgat cgagcccttc agcgcgtcga
    15481 cgtcggcggg cacgctatcg gggcagccgt cgtcgtcggc gtagccgttg atcgtctcga
    15541 gctggccctc gcacttgtcg gcgatgtcga gcacgcgatc gccgtcgttg tcgatgtcgg
    15601 ggcagccgtc ggcgtcctcg aagttgtcct ggtcctcggc gcgatcggga catcggtcct
    15661 gcgtgtcggg gatgccgtct gcgtctttgt cggcgggcgg gcccgcctcg acgaccttct
    15721 gttcctcgac gacttgcggg cgccgcggct tgccgggcga gagctcgatg ccgaagttga
    15781 tgtcgccttc gaggttgacc cagccgccga tcaggccttc gtcttccgcc gacgggatcg
    15841 ccgagatccg tgcgtcgaag cgcatcacga aacccttgag cgaatcgaag cgaatgccgg
    15901 cgccgaagta gccctcgccg acgatgccgg agttgaacgt cttgcgcgcg ctcgatatcg
    15961 cgatcggcga gccgccgccg acgaggatga acgggttcat gcggcgctgc ggcaacagat
    16021 cgatgcgcgc gtgcacgcgc ggctcgagcc acacgacgct cgtgttcacg ttcatctcgg
    16081 tggtcgtctt cgtcggtacg aatacgagct cgagctcggg caccagccac ggcaacccga
    16141 acggcttgcc gatgcgcgca ccgaagccga tcatgttctt gagatcgggg tgcgcgggct
    16201 cttcgtagtt gtagccgagc agtccgccgc tgctgaacag acgcggaccg aagaagccgc
    16261 cgagctgaag gtagtcgccg tcaccttcgc cttcggcgga agcgactgca ggcgcgagta
    16321 gcgtgagacc caaaactgcg agcgttaccc tgcgcatcac gcgcgccagt ataactgcga
    16381 attacgccgg gtggcgcgac gcgcacgtgg tcgtcaggct tggctaagtc ctggccttac
    16441 acgcccctta cacggcgccg tgacacaata aacgcagtgc ggatcctggt ggtcgaagac
    16501 gaaccgagct tgcgcgatgg catcgtcgat ttgctgtcgg gcgacggcca cacgatcacc
    16561 gcggtcggcg acggcgtcgc cggtgtcgag gcggggctgc gcgatccatt cgatctcgtc
    16621 gtgctcgatc tcatgctgcc gcggctcgat ggcatggagg tctgccgacg cctgcgtgcg
    16681 gcgcgtcccg gcatgccgat cttgatgctc accgcgcgcg gctcggaaga cgacaaggtc
    16741 cgcggcctga tcgaaggtgc cgacgattac gtgacgaagc cgttctcggc tcgcgagctg
    16801 ctcgcacgcg tgcgcgtgct cggtcggcgc gcacccgccg gcgagtcgct cgacgaagtg
    16861 caagtcgacg gcgcggtcat cgatctcgcg cgcatgacgg tcatgcgcgg ggatcaacgc
    16921 gtcgcgctca cgccgcgcga ggtcggcatc gtgcgctggc tctaccgcaa caaggatcgc
    16981 gtggtcacgc gtgccgagct gctcgaacaa gtgttcggcc agcgtggcga cctgcagacg
    17041 cgcgcggtcg acatggcgat cgcggtgctg cgcaagaagc tcgaggccga tccgcaaaag
    17101 ccgacgctga tcgtgtcggt caaaggcgcg gggtacgcat ggagactcgc gcgctgatcc
    17161 gaaagagcag tgtgtcgcgt gcgtggcacg cggccgcgct gctttcggtg ctgctcgcgg
    17221 cactgctcgg cgcctggttt ttctcgggct ggcacgacgt gcgaatgcgc cagcacgacg
    17281 tgcgcgaggc gccgcgccgc gctgccgacc agcgtgccga cgagctcgcg cgcgagctac
    17341 acggcgagct cgaagccctg atcacgcgcg aagtccgtcg cccgtacttc cactaccaga
    17401 atctgatgca cgacccgaag gcgtcggtcg gtgtctcggt ctcgccgtcg ccgctcgcac
    17461 gcaatcccga ggacaagctc gtcctcggct actgccagat cgacgcgaag ggcaaagcga
    17521 ccacgccgac gatcaa
//
LOCUS       DASUFX010000529         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1752143, whole genome shotgun sequence.
ACCESSION   DASUFX010000529 DASUFX010000000
VERSION     DASUFX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752143"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VFV99_21175"
     CDS             complement(<1..1384)
                     /locus_tag="VFV99_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX5061900.1"
                     /translation="MDQLKTIGPRAPGDEPIQHLKPQVDPSTLQHRQLLDGPFWQRIP
                     AYRTVDEATFLDHSWQAKNTITNPAKLLAAVQDLVPRSFYDDVEQGFHRSPMSIRVSP
                     YLLSLIDWNDPESDPLRRQFIPIASRLLPDHPKLTLDSLHEQADAPVPGLTHRYPDKA
                     LFLVLDTCPVYCRFCTRSYAVGVDTDMVDKVSLKATEDRWERAFRYISERSELEDIVV
                     SGGDSYQLKARQITLIGNRLLEMPNIRRIRFATKGPAVMPMKLITDHEWVDALTAVVE
                     RGRKLHKEVVLHTHFNHPNEITAITKRALDGVFERGIIVRNQSVLQRGVNDRVDTMQL
                     LVKRLGHCQVHPYYVYVHDLVKGVEDLRTTLQTALDIEKGVRGVTAGFHTPTFVVDAP
                     GGGGKRVAHSFEHYDRETGISVFAAPSVKPGFFLYYDPVDTLAPEIQEKWKHPAEQDR
                     MIEAAIAQAKL"
     gene            1549..3315
                     /locus_tag="VFV99_21180"
     CDS             1549..3315
                     /locus_tag="VFV99_21180"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5061901.1"
                     /translation="MRRLGPPRETAPTRQVVIAALTLLVLGSVGAVQAGLAGSQFMFD
                     LEQERPALTRVAPAAFDPHTPRLARRVFVVIIDGLRLDKSYELPFIDSLRRRGVDGEA
                     QSHYPTWSRPNYVSILTGVPPEASGVRTNHHSTPVALDSLMDRAKEAGLRTAMATDYD
                     VLPRLFLRARTPRSGEVTREVAPVLHHDDDVDPRVDEDSEVVDVVDDKETAAAVRAPD
                     ANLESPFDDARYAPWPGGFSEAGDALAGENADLVVMLLGAVDVAGHAHGGKSEQYRDA
                     ALIADHALSRALARVDLERDAIIITADHGHTNRGGHGGVEPEVLTVPFVAVGAGIKPG
                     ATPNDAHLIDVAPTVSALLGIAAPGHGLGRTLVEILAGDSAASMAWQVADTQRLTMTR
                     AVVAGAEATAEIDVLENRALRIGLVVCGAALAIGLATLLIRRRVLRLDIRVLLVSVPA
                     FFVVYYGLLGVLGQRWSPSLLPAKGHLAQTLAYYGIAAMAVQLLFSLWALRSQRTLAQ
                     RLAAANGIAWMGLMLAMIPAGLAWAFYPPPFVEVPPPLWLVLIPAIEVAVGCAAVSVA
                     LTLMLEVIVFASRAWHRAPPAG"
     gene            complement(3312..>3497)
                     /locus_tag="VFV99_21185"
     CDS             complement(3312..>3497)
                     /locus_tag="VFV99_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061902.1"
                     /translation="GTTLGNDAMCAPGFLCTTVTAGSYQCRRICNKTANTGCTGTQTC
                     LGFATPHTIDIEYGVCN"
BASE COUNT          542 a         1156 c         1176 g          623 t
ORIGIN      
        1 tgagcttcgc ctgggcgatc gcagcctcga tcattcgatc ctgctcggcc ggatgcttcc
       61 acttctcctg gatctccggc gccagcgtgt cgactggatc gtagtagagg aagaagcccg
      121 gcttcacgct cggcgcggcg aacaccgaga ttccggtctc gcgatcgtag tgctcgaagc
      181 tgtgcgccac gcgcttgccg ccaccgcccg gcgcgtcgac gacgaacgtc ggcgtgtgga
      241 agccagcggt cacaccgcgc acgcccttct cgatgtcgag cgcggtctgc agcgtggtgc
      301 gcaggtcttc gacgcccttc acgaggtcgt gcacgtagac gtagtacggg tgcacctggc
      361 agtgaccgag acgcttcacg agcagctgca tcgtgtcgac gcgatcgttg acgccacgct
      421 gaagcaccga ctggttgcgc acgatgatgc cgcgctcgaa cacaccgtcg agtgcgcgct
      481 tcgtgatcgc ggtgatctcg ttcgggtggt tgaagtgcgt gtggaggacg acttctttgt
      541 gcagcttgcg gccgcgttcg acgacggcgg tcagcgcatc gacccactcg tggtcggtga
      601 tgagcttcat cggcatcact gccgggccct tcgtcgcgaa ccgaatgcgc cggatgttcg
      661 gcatctccag caggcgattg ccgatcagcg tgatctggcg agccttgagc tgatagctgt
      721 caccgccgga gacgacgatg tcctcgagct ccgagcgctc ggagatgtac cggaacgcgc
      781 gctcccagcg atcttcggtc gccttgaggc tgaccttgtc gaccatgtcc gtgtcgacgc
      841 cgaccgcgta gctgcgcgtg cagaaccggc agtagaccgg gcacgtgtcg agcacgagga
      901 acagcgcctt gtcgggatag cgatgcgtga gcccgggcac cggcgcgtcg gcttgctcgt
      961 gcagcgagtc gagcgtcagc ttcgggtgat cgggcaagag gcgcgacgcg atcggaatga
     1021 actgacggcg cagcggatcg ctctccggat cgttccagtc gatcagcgac aatagatacg
     1081 gcgatacgcg gatcgacatt ggcgagcggt gaaagccttg ctcgacgtcg tcgtagaacg
     1141 agcgcggtac gaggtcttgc accgcggcga gcagcttcgc cgggttggtg atcgtgttct
     1201 tcgcctgcca gctgtgatcg aggaatgtcg cttcgtcgac cgtgcgatag gcggggatgc
     1261 gctgccagaa cgggccgtcc agcagctggc ggtgttgcag ggtcgaggga tcgacctgcg
     1321 gctttagatg ttgaatcggt tcgtcgccgg gagcgcgcgg accgattgtc ttgagttggt
     1381 ccatgggagt cctgcagtcg ttagagggtg tgccggtagg gaacagcgcc tcagacgacg
     1441 tcgggctggt gcgggacaga gcccaggtac cacatgagat ccaacgtata tcagggtgcc
     1501 ctcgttccgg ctagatgccg tgtgaagggc ggcgcgctaa cctgtggagt gaggcgcctc
     1561 ggcccaccac gcgaaacggc accgacgcgg caagtcgtaa tcgccgccct cacgctgctc
     1621 gtgctcggca gcgtcggcgc ggtgcaagcc gggctcgcag gcagccagtt catgttcgac
     1681 ctcgagcagg aacgcccagc actgacgcgg gtcgcgcccg ccgcgttcga cccgcacacg
     1741 ccgcggctcg cgcgccgcgt gttcgtcgtc atcatcgatg gtctgagact cgacaagtcg
     1801 tacgagctgc cgttcatcga tagcctgcgg cgccggggcg tcgacggcga ggcccagtct
     1861 cattatccga cgtggtcgcg cccgaactac gtctcgatct tgaccggtgt gccgccggag
     1921 gcgagcggcg tccgcacgaa ccatcactcg acaccggtcg cactcgactc gctgatggat
     1981 cgcgccaagg aggcgggcct gcgcactgcg atggccaccg actacgacgt cctgccgcgc
     2041 ctgttcttgc gcgcgcgcac accccgcagc ggcgaggtca cccgcgaggt cgcgcccgtg
     2101 cttcaccacg acgacgatgt cgacccacgt gtcgacgagg actccgaggt cgtcgatgtc
     2161 gtcgacgaca aggaaacagc agccgccgtc cgcgcgcccg acgcaaacct cgagtcgccg
     2221 ttcgacgacg cgcgctatgc accgtggccc ggtggattct cggaggccgg cgatgcactg
     2281 gctggggaaa atgccgacct ggtcgtcatg ctgctcggcg ccgtcgacgt cgccggtcac
     2341 gcacacggtg gcaagagcga gcagtaccgc gatgccgcgc tcatcgcgga ccatgcactc
     2401 tcgcgcgcgc ttgcccgcgt cgatctcgag cgcgacgcca tcatcatcac ggccgatcac
     2461 ggccacacga accgcggtgg tcacggcggg gtcgagcccg aggtgctcac cgtgccgttc
     2521 gtcgcggtcg gcgccggcat caaaccaggc gcgacgccga acgacgcgca tctgatcgac
     2581 gtcgcaccga cggtctccgc actgctcggc atcgccgcac cgggtcatgg cctcggccgc
     2641 acgctggtcg agatcctcgc cggcgacagt gccgccagca tggcctggca agtcgccgat
     2701 acccagcgcc tgacgatgac gcgagcggtg gtcgcgggcg cggaagcaac cgccgagatc
     2761 gacgtgctcg agaaccgcgc gctgcgcatc ggcctcgtcg tctgtggtgc cgcgctcgcg
     2821 atcgggctcg cgacactcct gatcaggcgg cgcgtgctgc gcctcgacat ccgagtgctg
     2881 ctcgtctcgg tgcccgcgtt cttcgtcgtc tactacggtt tgctcggtgt gctcggtcag
     2941 cggtggtctc catcgctgtt gcccgcgaaa ggtcacctcg cacagacgct ggcctactac
     3001 ggcatcgcgg caatggcggt gcagctcttg ttcagcctgt gggcgctgcg cagccagcgc
     3061 acgctcgccc agcggctcgc cgccgcgaac ggcatcgcgt ggatgggcct catgctcgcg
     3121 atgatccccg ctggcctggc gtgggcgttc tatccgccgc cattcgtcga ggtaccgcca
     3181 ccgctctggc tcgtgttgat tcccgccatc gaggtcgccg tcggctgcgc cgcggtgtcg
     3241 gtcgcgctca cgctgatgct cgaggtgatc gtgttcgcct cacgcgcatg gcatcgcgcg
     3301 ccgccagctg gctagttaca gacgccgtac tcgatgtcga tcgtgtgcgg cgtggcgaaa
     3361 ccaagacatg tctgcgttcc ggtgcagccg gtattcgcgg tcttgttgca gatgcggcgg
     3421 cactggtagc taccggccgt caccgtcgtg cacagaaagc ccggcgcgca catcgcatcg
     3481 ttgccgagcg tcgtccc
//
LOCUS       DASUFX010000530         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_84052,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000530 DASUFX010000000
VERSION     DASUFX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84052"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFV99_21190"
     CDS             complement(<1..415)
                     /locus_tag="VFV99_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5061903.1"
                     /translation="MAEILVEEKNGVVWVTLNRPEVRNALSRDVNLQLTEIATGFEQR
                     DDVRAVVLTGAGEKAFCAGADLKERKGVSANETAPYLNAIAGAIESWAAIKQPTLCLM
                     NGSAYGGGLELAMACDFRVLVEGSEVGLTEVRLGIM"
     gene            complement(428..1366)
                     /locus_tag="VFV99_21195"
     CDS             complement(428..1366)
                     /locus_tag="VFV99_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5061904.1"
                     /translation="MKLPAEVTIYEVGPRDGLQNEARNVPTADKIRFIDALVASGIRA
                     IEITSFVSPKWIPQLADSAEVARGVQRPPGVRMSALVPNRRGLDTALASGMTEIAVFM
                     SVSETHNKKNVNKTVAETLAAFDEVVPAALAANVKVRAYLSTCFGCPYEGEVDPDRVV
                     ELVGRLRDMGVFQVSVSDTIGVANPFQTEDVLTRVLAKHPVESIAVHMHDTQGTALAN
                     VVVALQLGITTVDSAAGGLGGCPYAPGAAGNLATEDVVGMLHSMGVKTGIDLDKLVDA
                     SRTASTFVGHELPSKYLKAHVGKQARARRRTERLGS"
     gene            complement(1363..2049)
                     /locus_tag="VFV99_21200"
     CDS             complement(1363..2049)
                     /locus_tag="VFV99_21200"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5061905.1"
                     /translation="MIRGFLFDLDGTLVDTERESGEAMARALLRGQQIAIEQSDRDFI
                     IGRSWMAIYDRLKSRYPQLAWTRDEVIAQTAAIREEVFAEVGVTVMPHARETLAWTRT
                     YPRAIITGSARAEAMQVIPLIGPDSHFDTIVAAEDVPRSKPHPDGYQKAMGVLGLPPH
                     ECIVIEDSESGIEAGRSAGCLVVAVRAGNFGGWRQDHAHHVIDTLAELTPSLVEELTG
                     KYLSSMGGAP"
     gene            complement(2202..3128)
                     /locus_tag="VFV99_21205"
     CDS             complement(2202..3128)
                     /locus_tag="VFV99_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061906.1"
                     /translation="MKKLYIALGISLLGCGTDNSGPEVLPDLTMPEAPKNGVQVLTPV
                     FENIQPGSDNEVCTWTDIVFDHEVDVKSTLGYQNEPPGHHAILFYTTEKQPPGTQRIC
                     TDTDMASFRYLTGNGANGMLNEAPGDLVFRIPKGAQLVVNSHYLNAGDEVLRGQSVIN
                     VNFADAGPHTPSGSLAIVNTGLEVAQGQSTQNMHCTYEKQYKLWYLIPHEHRWGSHIT
                     VDITRSGEKKRMFDLDWQEQYTFHPPENRYETTEPYLINPGDTIDVACSWNNTEGRTL
                     PFGFEMCVVFGQFIDEQGLGGWACDHGSWTEF"
     gene            complement(3142..3483)
                     /locus_tag="VFV99_21210"
     CDS             complement(3142..3483)
                     /locus_tag="VFV99_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061907.1"
                     /translation="MRAAMLVFILVAGCGGEEGNKTPDANVDAYFSECGHPGDVGNEL
                     GIGKFCPNGLSDCSNTPSAPLCSSLGSSTTHFCTKTCQMGSTDQCGTGAECTCNNSNQ
                     CGCTPSSCLGP"
     gene            3659..>4482
                     /locus_tag="VFV99_21215"
     CDS             3659..>4482
                     /locus_tag="VFV99_21215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5061908.1"
                     /translation="MACANRAKRSIDLYDSGDYAGASRAADEGLASHPNDDGLWAMKV
                     RASIALGDIEGTAKAYEMYVSHRGGDDKELLRDLATATLGQALASPSAKMKMAAIEAI
                     ERIEIHSLTDQVAERMGDDDDRVAATAAVALLNGMPGAPQVLDDMLRSENAEARRIAV
                     EGVGRKVALKGKGNRVSQLAAGDLETAAKDSDPRVRRAAIRWLGMIKDKDAVEVLMQH
                     MKDPDDSVRAAAASALARIGVGNLAALGKQAIADRSLAVRMAAIDLYAAANADADLV"
BASE COUNT          770 a         1485 c         1432 g          795 t
ORIGIN      
        1 gcatgatgcc gagccgcact tcggtgagcc cgacttcgct gccctcgacg agcacgcgga
       61 agtcacacgc catcgcgagc tcgagcccac caccgtacgc cgagccgttc atcaggcaca
      121 gcgtcggctg cttgatcgcg gcccagctct cgatcgcacc ggcgatcgcg ttcaagtacg
      181 gcgcggtttc gttcgccgac acacccttgc gctctttgag atccgcgccc gcgcagaacg
      241 ccttctcacc tgcgccggtc agcacgaccg cgcgcacgtc gtcgcgctgc tcgaagcccg
      301 tcgcgatctc ggtcagctgg aggttcacgt cgcgagacag cgcattgcga acttcgggac
      361 ggttcagcgt cacccagacg acgccgttct tttcttcgac gaggatctcc gccatgcgcg
      421 gaagctatca gcttccgagg cgctcggtgc ggcggcgggc acgcgcttgc ttgccgacgt
      481 gcgccttcaa gtatttcgac gggagctcgt ggccgacgaa cgtcgaggcc gtgcgcgacg
      541 cgtcgacgag cttgtcgaga tcgatgccgg tcttgacgcc catcgagtgc agcatgccga
      601 cgacgtcttc ggtcgcgaga ttgcccgccg cgcccggcgc gtacggacag ccgccgagcc
      661 caccggccgc ggagtcgacg gtcgtgatgc cgagctgcag cgcgacgacg acgttcgcga
      721 gtgcggtgcc ttgcgtgtcg tgcatgtgca ccgcgatcga ctcgaccgga tgcttcgcca
      781 gcacgcgagt gagcacgtcc tcggtctgga acggattcgc gacgccgatc gtgtcggaga
      841 ccgagacttg aaacacgccc atgtcgcgca gccggccgac aagctcgacg acgcgatccg
      901 gatcgacctc accctcgtac ggacagccga agcacgtcga caggtacgcg cgcactttca
      961 cgttcgctgc gagcgcagca ggcacgacct cgtcgaacgc ggccagcgtc tcggcgacgg
     1021 tcttgttcac gttcttcttg ttgtgcgtct ccgacaccga catgaacacg gcgatctcgg
     1081 tcatgcccga cgcgagcgcg gtgtcgaggc cgcggcggtt cggcacgagc gcgctcatcc
     1141 gcacgcccgg tggccgctgc acgccgcgcg cgacctcggc cgaatcggcg agctgcggga
     1201 tccacttcgg cgacacgaag ctcgtgatct cgatcgcgcg gatccccgac gcgacgagcg
     1261 catcgatgaa acggatcttg tccgcggtcg gaacgttgcg cgcctcgttc tgcaagccat
     1321 cgcgcgggcc gacctcgtag atcgtgacct cggcgggcag cttcacggtg cgcctcccat
     1381 gctcgaaaga tacttcccgg tcagctcctc gaccagcgac ggcgtcagct cggcgagcgt
     1441 gtcgatcacg tggtgggcat gatcctgccg ccaaccaccg aagttccctg ccctgaccgc
     1501 gacaaccagg cagccagccg atcggccagc ttcgatgccc gactcgctat cctcgatgac
     1561 gatgcactcg tgcggcggca ggccgagcac ccccatcgcc ttctggtagc cgtcgggatg
     1621 cggcttgctc cgcggcacgt cctcggcggc aacgatcgtg tcgaagtgcg aatccggccc
     1681 gatcagcggg atcacctgca tcgcttcggc gcgcgcgctg ccggtgatga tcgcgcgcgg
     1741 gtacgtgcgg gtccacgcga gcgtctcgcg ggcgtgcggc atcaccgtga cgccgacttc
     1801 ggcaaagact tcctcgcgga tcgccgccgt ctgcgcgatc acctcgtcgc gtgtccacgc
     1861 gagctgcggg taccgcgact tcaggcggtc gtagatcgcc atccacgagc ggccgatgat
     1921 gaagtcgcga tcggattgct cgatcgcgat ctgctggccg cgcaacaacg cgcgcgccat
     1981 ggcctcgccg ctctcgcgct cggtgtcgac aagcgtgccg tcgaggtcaa acaaaaagcc
     2041 gcggatcact ccgcggactt atagcaggcc tcgggcctca gacctcaggc ctcgggtcag
     2101 atccgaaaac agaaacagcc ggctccaacg agtcggctgc tgatttctga ttctggtttc
     2161 tgggttctgg ctgaggtctg aggtctgagg cctgaggcct cttagaactc ggtccagcta
     2221 ccgtgatcgc acgcccagcc accgagacct tgctcgtcga tgaactgacc gaacaccaca
     2281 cacatctcga agccgaacgg cagcgtgcgg ccttccgtgt tgttccacga gcacgcgacg
     2341 tcgatcgtgt cgcccgggtt gatcaagtac ggctcggtgg tctcgtagcg gttctcgggc
     2401 gggtggaacg tgtactgctc ctgccagtcg agatcgaaca tgcgcttctt ctcgccgctg
     2461 cgcgtgatgt cgaccgtgat gtgcgagccc cagcggtgct cgtgcggaat caggtaccag
     2521 agcttgtact gcttctcgta cgtgcagtgc atgttctggg tcgactggcc ctgcgcgacc
     2581 tcgagaccgg tgttgacgat cgccaacgag cccgacggcg tgtgcgggcc cgcgtcggcg
     2641 aagttcacgt tgatcacgct ctggccgcgc agcacttcat cgcctgcgtt caggtagtgg
     2701 ctgttgacca cgagctgcgc gcccttcggg atgcggaaca cgagatcgcc gggcgcctcg
     2761 ttgagcatgc cgttggcgcc gttgccggtc aggtaccgaa agctcgccat gtcggtatcg
     2821 gtgcagatgc gctgcgtacc cggcggctgc ttctccgtcg tgtagaacag gatcgcgtgg
     2881 tgacccggcg gctcgttctg gtagccgagc gtcgacttca cgtcgacctc gtgatcgaac
     2941 acgatgtccg tccacgtgca gacttcgttg tcggagccag gctggatgtt ctcgaagacc
     3001 ggcgtgagga cctgcacgcc gtttttcggc gcctcgggca tcgtgagatc cggaaggact
     3061 tccgggcccg aattgtcggt gccgcagccg agtaacgaga tgccgagcgc aatgtagagc
     3121 ttcttcatcg ttatcccctc tctaaggacc aagacacgaa gacggcgtgc aaccacactg
     3181 gttgctgttg ttgcaggtgc attcggcacc ggtgccgcac tgatctgtcg agcccatctg
     3241 gcaggtcttc gtgcagaagt gcgtggtcga gctaccgaga ctggagcaga gcggagccga
     3301 cggcgtgttc gagcaatccg agagcccgtt cggacagaac ttgccgattc cgagctcgtt
     3361 gccgacatca ccgggatgac cgcactccga gaagtacgca tcaacgttcg cgtcgggcgt
     3421 cttgttgccc tcttcgccgc cgcaaccagc gacgagtatg aacacgagca ttgctgcgcg
     3481 catgagccct cctcggtagg cggtgccgct ataacgcgat gctttgtgca ccctcaagca
     3541 caaacttgtg aaacctcatg gctgtaacca tccgtgcaaa ggggtgcaca gaccccgtgt
     3601 catgctcaca ggagtccgca tgcgccgtat cgctctagct ctcgctcttt tggcccctgt
     3661 ggcatgcgca aaccgcgcaa aacggtcgat cgatctctac gactcgggcg attacgcagg
     3721 cgcatcgcgc gccgccgatg aagggctcgc gagccatccc aacgacgacg gtctgtgggc
     3781 gatgaaggtc cgcgcgtcga tcgcgctcgg tgacatcgag ggcactgcga aggcgtacga
     3841 gatgtacgtg agccatcgcg gtggcgatga caaagagctg ctccgcgatc tcgcgaccgc
     3901 gacgctcggc caagcactcg cctcaccatc cgcgaagatg aagatggcgg cgatcgaagc
     3961 gatcgagcgc atcgagatcc actcgctcac cgatcaggtc gccgagcgca tgggcgatga
     4021 cgacgatcgc gtcgctgcca ccgcggccgt cgcgctgctg aacggcatgc ccggcgcgcc
     4081 gcaggtactc gacgacatgc tgaggagcga aaacgcggaa gcgcgccgca tcgcggtcga
     4141 gggcgttggt cgcaaggtcg cgctcaaggg caaaggcaac cgagtcagcc agctcgctgc
     4201 gggcgatcta gagaccgcgg cgaaggactc cgatccgcgc gtgcgccgcg ccgcgattcg
     4261 ctggctcggc atgatcaaag acaaggacgc ggtggaagtg ctgatgcagc acatgaagga
     4321 tcccgacgac agcgttcgcg cagccgccgc gagcgcgctt gcacgcatcg gcgtcggcaa
     4381 cctcgccgcg ctcggcaagc aggcgatcgc cgatcgatcg ctcgcggtgc gaatggctgc
     4441 gatcgatctc tacgctgcgg cgaacgccga cgcagatctc gt
//
LOCUS       DASUFX010000531        30568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_502760,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000531 DASUFX010000000
VERSION     DASUFX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 30568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502760"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..864
                     /locus_tag="VFV99_21220"
     CDS             370..864
                     /locus_tag="VFV99_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061909.1"
                     /translation="MYPDTRITDGTGRVDQMLIATPWHRTWDTAGDQERAAAAHRSEA
                     QAIYAAYDEACAGRSSSDISVSPIVRYGQGGGVTPDGVELYLSTAAGPPERLLADLKC
                     HRAWMRLAPANMDACPLDLAGLLIDARGSGEGITLTLSVRDRSLIPELQRRAAHDLEI
                     GHHH"
     gene            complement(861..1727)
                     /locus_tag="VFV99_21225"
     CDS             complement(861..1727)
                     /locus_tag="VFV99_21225"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5061910.1"
                     /translation="MSDANPRGVRRMLQLRQFPLFETIDLDELATIAENLVEITIPAG
                     TQIVAAGDRVHGVHLILAGRIESQPHGQTWSAHQVFGALEVLAHRAAAHSAVAASDVQ
                     ALYLPAGEVGEVLEDNFGVLLATVRELAGRLLAAAPPPARTCGCAAGPLGLVERLIVL
                     RQLLPFTSARLQALATLAHASEEVQWRAGAVIAHAGEPASGGLIIIDGGARALRDDGE
                     QLFEPGAAIGHLETLAGLPHRATIETTQPVRALRSGAPAIFDVLEDHTDVALAMTATF
                     AGALLDATAQLN"
     gene            complement(1720..4365)
                     /locus_tag="VFV99_21230"
     CDS             complement(1720..4365)
                     /locus_tag="VFV99_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061911.1"
                     /translation="MTKMRRIAVVPAALLFGIMLAHALLETARDALFLARLGPHALAS
                     AYLAIAAVAMLAVVAVRRWGGVRDPRRMLLTFLVGAIAGTLLIGVLIPIAPPFVFVLY
                     VWTGLVATLVVPSFWTLIDRSLQIAEAKRAFAVIGAGGVLGAMTGSALAGLLGRIVPP
                     HYLVTAGAIAFTLTLLASFVYAPRTLDDTLPPVHKRVEALSRRSRRYVRLLVAVGLIS
                     TIALTLADLTFKRVLAERFAAADLATAFGAIYTVLNFVGLLILLVVTPRLLARWGVGS
                     ALMVLPILLIATASGFALTGTLLAIIALKLCDGGLRHSLQRVASEILYLPLPSLVRDG
                     GKPIADAISQRGGQAIAALLWFLIASLAIGTQLLGFVVAATSVAWLLAVRIAKRAYIA
                     QFADTLRAGAIERDVRAPALDGDSAALLTEALASPDEPEALAALELLARGGRVPALVL
                     YHPREGVVRRALLLLEGQLRADVARVLRHLIEHSDPRIRAAALIAASRTHSNRAQLEA
                     AAADPNPDVRAAALVGLGDHADCEVLVESGVAALLNGSLADRIALAHAIGYTPHDRFR
                     RLLYQLLALREVPVMREVLRVLARAPSLVDLDRIVPLLEDPHVRGDVRRVFTATGSRG
                     LDKLMAALDDARTPIGVRRHVPRTISRFRSRAAASALVARLLREPDGTTEFKILRALG
                     RMRANNPLLPIDPAILHEYVRRSIADAARYTTLLDAFVVAGDTSPGAELIRELLGEKR
                     RWAIEHTFRALGILHPRHALRSVHDAINRGDGNRRSAAREIVESLLPVDQRGALLAVI
                     DELTPEQRRSQLGDLAVGPFPTYEALLGALLADSSESLKCVVAHHVAERHLVALRHDL
                     TRLRPMVGPPLVIYAFDQAIARLDV"
     gene            complement(4362..5948)
                     /locus_tag="VFV99_21235"
     CDS             complement(4362..5948)
                     /locus_tag="VFV99_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061912.1"
                     /translation="MRFANAPPARAVNDRANVAAQPVPRAFVRWLYNYDGQFQRRITR
                     ALELHRPERARGINALDEVPNSTWFTNRIGVRDVSPEEIATAPGGVGSPEAFKPWTIV
                     STKVGGMSVGFIIKDTRGEKFLLKFDPRGLPEAETATQVIVGKLLWAFGYNVTEDYVL
                     YVHKQDLVLAPDAVVKDIFGHKSPLGQRELDKRLALIETAADGRIRVLVSHWLPGKPL
                     GGHAAEGVRGDDPNDRIPHELRRDLRGAYAVFSWLDHFDIHEGNMLDVWVGDPADPKR
                     HYVKHYWVDYGVALGFSAKKNVDARYGYEFYWDPAAMARSLLSFGALERPWEQRKWRD
                     VRGLGLYETDLYDPGAWVSSSAAYVPIYVADRIDKFWASKILIRFTRDQIKAAVDSAQ
                     LSDPKAAAWLVDALIARQRATARYWFERVNPLDEPAIADDTLCFKDLAIAHAFAPAGN
                     THYAITTYDHAGTQLAATDAPAGDSGVTCTRLGLAADRNGYTIVRVDTTRPQFTGTTY
                     FHLARDPTTQAARVIGVWRP"
     gene            complement(6008..7354)
                     /locus_tag="VFV99_21240"
     CDS             complement(6008..7354)
                     /locus_tag="VFV99_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061913.1"
                     /translation="MKRVVIAVSMLTTGLARADDPPPPRDEPSAVDVETAPVPGTESG
                     RTDPGDHDSVLRDIGQGALFVPRIAVEVALSPVRASVWALDRFQLVDRFKRVFFDESE
                     TYGLYPTLVLDQSYGLTVGARFVHRNLFGEREHLALRGGTGGEYRAQASVGLRSGERF
                     GERTQLELRGELERRPADAFYGIGNTEGSMIAYHREELFRASTRVDIRAISDLHVRAA
                     AALTDIAYADSSDGPPIDMIYTASSLTGWPGTRNLYSELELRWDSRRVSTPFGGHHVL
                     DTGSLLAVYAGPVHALDGGVDYWRYGGEAQQFLNIGAGPRALATRLYAEGVTGDTQSV
                     AFTQLPELGGKTLLRGYPRDRFRDRFAAVGSLEYMWDLGNYPMASLFIDVGRVFPSPR
                     DIEPTNLRVGYGFSLQLHDVRRFIASVSVASSIDGGVFFNLAFDPVFDIEPRVEDR"
     gene            complement(7351..8730)
                     /locus_tag="VFV99_21245"
     CDS             complement(7351..8730)
                     /locus_tag="VFV99_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5061914.1"
                     /translation="MTSGTASLTFLDRLLRPFSKVRPGEGVIAALMLVCVFLILTSYY
                     VMKTAREGLILSGGTWGLRGDELKTYANGAMALLLVGVVPAYGMLANRTRRIRLINVC
                     YAIVIASLLAFFVLGRAGVPIGLAFFVWLGLVSVFLIAQFWSYANDLYTEEQGKRLFA
                     IIATGGSLGAIFGPRIAKLANTFTLLLVAALILGASIALFNVIEHVNNRHGRDHRSVE
                     PITGAGGFALVLRDRYLLLIACLLLVANLVNTTGEYVLSNAAREHAMELVPARIGVDA
                     SVINAERRELIKAFYSDFFYWVNVTSFLIQAFLVSRIIDKLGVRRALFALPLIAFGAY
                     AMIGTVGGLMLVRTAKIAENGTDYSLQNTVRQALFLPTDRVVKYKAKAAIDTFFVRAG
                     DTLSAVLVGIGIHELAFTGRDLALVNLILITVWLAIAGGIARRHRLISRESEPPPPPV
                     LHPLPGAHA"
     gene            8954..10183
                     /locus_tag="VFV99_21250"
     CDS             8954..10183
                     /locus_tag="VFV99_21250"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5061915.1"
                     /translation="MLYQRVISISILGLAACGNVSGHVSSTKTGDTTHPAERGSPVDA
                     LHAACGDGAATPFGTNTVKRQPYLQQVTASGAMLGWVSTQPAGEHVSVTKPPDAMATL
                     APGVVEDYALRSAGENQMWSTLDKLEPSSIYCYQLANDTQTLNAPTGFRTAPAADDPA
                     PVRFLAFGDSGGGGSDQYALMEQMYTVPFDLMIHTGDIAYDDGTIEQYEDNVFGVYAD
                     LFRSIPFFPASGNHDYHTQAGAPFRDVFNLPGANGEKWYSYDWGRVHFVALDTEADYA
                     TQMKWLEQDLAASQAPWKIVYMHKPPYSSGIHGSDTRLRNMLSLIAERGGVQLVLAGH
                     DHDYERMKPQNGIAYVVTGGGGIGTRGVGSSSFTEFSVDVIHFVYVEVGVDELTLHAI
                     DATGKEFDSMVVPRTRA"
     gene            10390..11010
                     /locus_tag="VFV99_21255"
     CDS             10390..11010
                     /locus_tag="VFV99_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061916.1"
                     /translation="MRAHMTVHLEEAAALQAAIARGWLVDARERARWFASHDMEAPPS
                     WQPYLDDMRYAAMRIARARDVATAGAQIGLLGRACGACHEANAATPAFQYDPPPTDDA
                     TLEAQMRRHQWAAARMWEGVVGPADAAWQDGARVMATTRLDVARTAHEKPNADVFELA
                     ERLRDQATQALTMTARDARARFYGEMMETCASCHSIVRPIARAAAR"
     gene            11021..12055
                     /locus_tag="VFV99_21260"
     CDS             11021..12055
                     /locus_tag="VFV99_21260"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX5061917.1"
                     /translation="MKAARIHAYRQPLTLDEVQMPIARPGEVIVKVAGSGFCHSDLHV
                     IDGELPVLPRMPLTLGHENAGWVAETGAGVSGVREGDPVVVYGGWGCGRCDYCVTGHE
                     QLCGAPEWCGLSQWDGGYAEYLRVPHERYLVKLTKLDPVWAAPLADAALTPYRAVKKA
                     LPLLEPDHAVLAIGLGGLGQYGLKLLRLLSGAPIIAVDTSIEKRRIAYELGAETVIDG
                     AAPDLVDRINAATHGNGVTAAFDFVGAEHTLDTAVRATRALGKVFQVGLGGGTARLKV
                     LDNAKFEVGFECTLWGTIKELREVVALVEDGRLALGQAETAPLDQINNVYARLKRGDV
                     RGRVIITPSA"
     gene            complement(12056..12202)
                     /locus_tag="VFV99_21265"
     CDS             complement(12056..12202)
                     /locus_tag="VFV99_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061918.1"
                     /translation="MKWIVVAVLLAACGAQQVKVDPVKVEPIHITVDVNLHDKSADAS
                     NPQK"
     gene            complement(12199..13188)
                     /locus_tag="VFV99_21270"
     CDS             complement(12199..13188)
                     /locus_tag="VFV99_21270"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEX5061919.1"
                     /translation="MGVAAAAGVAYVLLPIVVEHHIRAGLVERGFPHARLEVASIGLQ
                     HMQLRNVHLDDGLDLGTLDFDRGLSLLWRDVNDVSITHASVSTAALARLAPALTRTAN
                     AALPFRHAKVADSTLDVQGTKTRMAGTASASGKALEITIDVRDPSPKGWAVSLEAKVD
                     AERTLAVSGKAHVLGGEVEVEPFIVRAGSDVVVHARGLQLAQLLEPTEHLTGSGLVDG
                     TVALRLDRDGWSLDSGELHARKGGSLQVTDRTWRDRLTKEQSPFAVHAKLLDAVTDFQ
                     FDTLTAELAPPGAGTDLRLSTRGRGRRNHQELDVAIGIRGARDVAARLPGAVP"
     gene            complement(13627..16191)
                     /locus_tag="VFV99_21275"
     CDS             complement(13627..16191)
                     /locus_tag="VFV99_21275"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX5061920.1"
                     /translation="MDAELPASVTGLSSAEVAARQRAVGRNEMPSADRRTLLQHIGGV
                     LREPMLLLLLVATAIYIAFGDALEAGALGVSVLAIVAITLAQERRTERALDALRELAS
                     PIARVYRDGRWQEVDARELVPADVIHLSEGDRIPADALLRAGTPLAVDESLLTGESVP
                     VLHRPDPAVRALGRPGEEGASVFSGTLVGSGNAVAEVMLTGPRTEVGKIGAALGGIEL
                     ARAPLHREVATIVTRVAIVAIGLCVLLAAIYIVTGAGILEALLAGITLAMSVLPEELP
                     LVLTIFLTLGAWRIAKHHVLARRAAVIETLGAVTVLCVDKTGTLTQNQMAIRRVMSLE
                     GSANIAVDATKLPESVHEAIEFGALACPQQPVDPMDRAFVRLADQALAKTEHVHPAWR
                     WVREYPLSPGLLAVTHVWRDDEQRTVVATKGAPEAIIDLCHLGPEQAAYWRAQADLMA
                     REGLRVLGVARAYHTHGGAKTPTQPHDFAFELVGLVGLADPLRPETKQMIEQCHEAGV
                     RIVMITGDHPTTARAIARAAGLDAGSVLTGPDLEQLDEEALAYALEHTEVIARAAPAH
                     KLRIIHALRLAGEVVGMTGDGVNDAPALKAADVGIAMGHGTEVAREASGLVILDDTLA
                     AIAAAIRAGRTIYDNLRKVASYLLSVHVPIVGVALLPPLLGWPPLIGPIHVVLLELVI
                     DPTCSIVFELDPPARDVMQRKPRARTEHLFDAKRVGFALLLGVAAALGPLAVAAVAHN
                     AGYAADTVRALAFVALIAADLMLVAASRERLARGSHVGKNPAIFWMLVAVTGVFALTL
                     VIPGLRTLFGFAPLGMPHVVAAALAGALPVALTSLLHAARPGEGRTTRAGHAASQA"
     gene            complement(16221..17294)
                     /locus_tag="VFV99_21280"
     CDS             complement(16221..17294)
                     /locus_tag="VFV99_21280"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5061921.1"
                     /translation="MRSLIALGLVGCANAAPGKPITKVELGEQLFNDTRLSEPAGQSC
                     ADCHDAKLAFTDPENDRVSAGVIRERFGVRNAPTAMYASFVPPLHRDAHTQSMVGGLF
                     WDGRADSLETQALSPLTNPLEMNNPDRGAVVAKIRRYYGRQFHDVFGKDALTDEATAL
                     AHLGDAIAAFERTSGFAPFSSKYDRYLAGTVELAAAEARGLAIFEDPARGNCASCHPS
                     RPGPDGSPPLFTNFSYANIGLPRFADSPFYMLPTALNPDGAAFIDRGLAKTTGDPRDD
                     GLFRVPTLRNIAKTSPYGHNGYMRRLDEMIELLAGTCLRPGTCNPPAPEVVANAQRVR
                     TGTTLSKQDIADLLAFLRTLDDE"
     gene            complement(17348..17776)
                     /locus_tag="VFV99_21285"
     CDS             complement(17348..17776)
                     /locus_tag="VFV99_21285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5061922.1"
                     /translation="MKRILVAVDGSPRAQVVLDAAARLANLTHAKLVLFRAVGVPPEL
                     PADLISLPPIRLEETLRSTALASLDRIASTVSPALIESITTGVGTAWDAIVREAKEVD
                     ADLIVIGSHGYSGLDRLIGTTAAKVVNHADRNVLVVRTVL"
     gene            complement(17773..18204)
                     /locus_tag="VFV99_21290"
     CDS             complement(17773..18204)
                     /locus_tag="VFV99_21290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5061923.1"
                     /translation="MKISTILVPIDFSSTSETALDYARELAPKLDARLHLLTVIGVPS
                     YGVPELGVGVTATMIDTMITDNQAALDQLVRDRKLTAQTHVRAGDARDVILQTAEELK
                     ADLIVMGTHGRRGISRALLGSVAETVVRTSTVPVLTVRGKK"
     gene            complement(18201..18590)
                     /locus_tag="VFV99_21295"
     CDS             complement(18201..18590)
                     /locus_tag="VFV99_21295"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5061924.1"
                     /translation="MPPVSRYMTNHPYAISPHEKLSSARHLMATRDIHHLPVMEHDEL
                     VGIVSDRDLHPVHPLHDMTVGELMTEDVAHVSADTPMDEVIDLMERKHCSSVVVLGLK
                     GVEGIFTVTDALRALGDLLRRTVEDRP"
     gene            18758..19369
                     /locus_tag="VFV99_21300"
     CDS             18758..19369
                     /locus_tag="VFV99_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061925.1"
                     /translation="MNPVLLVSFAALPWLQGFFAPTSETEQGSRDVLAELAEWHEPVD
                     DCSASAYGGLEMRADVSASEGQERVLASYTQGVFVFDRDQHLLAQAPAFACAGSSDEL
                     VAMAAGDASIGTPVIALAATTGGRATNVTWLTLYRVAGSGELQPVFNGEVERHDGDTA
                     RTGVVTLIPGGLIYRDTRGSIGLWLYSTELGRYVELFTSQPNV"
     gene            complement(19380..19823)
                     /locus_tag="VFV99_21305"
     CDS             complement(19380..19823)
                     /locus_tag="VFV99_21305"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5061926.1"
                     /translation="MPVPFPHHYQTWLVRTLSSRARLEAPPRPLISAGPPPEFDGDAT
                     AWSAEQLLLSSIGMSVLTTFEALAARARVDLLAWEARVGGVVDRSEHGLAFTKFTVDI
                     DMEVSDVERARAVLDETKQHCLIANALKTPLEIDAKIRTPLRKAG"
     gene            20022..21053
                     /locus_tag="VFV99_21310"
     CDS             20022..21053
                     /locus_tag="VFV99_21310"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5061927.1"
                     /translation="MHLTLLILLVWIASTYLLHGANLSTTAFGLVLVLTIFAIIVVHE
                     LGHALVARRYGIATRDIMLLPVGGISSLERMPEKPTQELAVAIAGPLINVVLAGLLYI
                     GIKITGGSTHITEPMSIGAAFATQLMWINIGLAVFNLLPAFPMDGGRVARSLLAMRMG
                     YDRATDIAAQLGKFFAILIGFFGLFYSPILILIAFVVWSGASQERALVHLKTAISGVP
                     VSAAMLRRVDSVSPEQPLEDAAAMLLRGGLNQLPVIDHGEPVGVITRSDVATALSHAG
                     PDATVANAPQHAIVMVSPADSLDSVLDKLRQSPDSVALVVDHGHPVGVLTAEHLAAYV
                     ALHSQRRAA"
     gene            complement(21063..22160)
                     /locus_tag="VFV99_21315"
     CDS             complement(21063..22160)
                     /locus_tag="VFV99_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061928.1"
                     /translation="MRALVVVALAGCSQWFGLDEPEHTTDARTIDGAPLCLGSGKLEI
                     CVDSAPTMILNLDGTLDTTQDCPLRKDVNGAMVCVLAGSRVTVDTSFTAVGALPIVVF
                     AVGDISIDGTLDVASHGGTVGAGANAASCPMLPDGTASTNGGGGGAGGSFGSLGGHGG
                     AGDMGQTQGAAPVGGSFAANTLHGGCAGGRGGRGGGASLEGAAGNGGGGVYLLAGGSL
                     MIAGVVNASGGGAGGGSASKGGGGGGGSGGMILLHAATIVLGMNGALMANGGGGGGGS
                     DNAASGNPGGETTEYNKAAPGGFGGGTTSCAGATPGCGGDGAIQGKPAANGGNDGTGA
                     GAGAGGGGLGVISVLSGQSLTGKVSPQPTMM"
     gene            complement(22194..23444)
                     /locus_tag="VFV99_21320"
     CDS             complement(22194..23444)
                     /locus_tag="VFV99_21320"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061929.1"
                     /translation="MFGASALQAQPAGGGGAVIDVSGTKGGKYPIALPTAPEGDATAK
                     EISQVESFDLNVAGPFKVLDPQGFLADLKSEGTGIDPQKWKDVGAYGVIKYKATGDAI
                     DFKLYEVSKGNKPTLEKTYKRAGTDTRTIVHRWCNEVVKYYTGEPGFFGSKIAFTQKG
                     KGTSAILAMDFDGNGVYKVSNNSSTNILPAWSPSGGQIAYTSFMRANPDLYVAPAGGG
                     RPKQLSKQRGMNTGASWSPDGSKIAVTLSKDGNPEIYIINASDGSVISRLTKDKAIDT
                     SPAWSPNGAEIAFVSDRNGGPQIFVMPASGGAAKQVSTNGSYNTTPTWSPKPGKRIIA
                     YTTRDGGKYDIVALDLDSKAMTRITQNEGSNEEPSFSPNGRAIAFSRAGQGIFIANAD
                     GTGKAVKVYSGSTTGVDWGPAPKD"
     gene            complement(23462..24166)
                     /locus_tag="VFV99_21325"
     CDS             complement(23462..24166)
                     /locus_tag="VFV99_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061930.1"
                     /translation="MSLNDGEMQAVSLIGAAILSASLIAPVVYFSAAAVHPPKYLDDR
                     QVLEATIAYKKSPTKQPQKKFDQVEDVKPVGVSHDEKKKVEEKKDDKKKDPKIDPNDP
                     FKGIHRNDGEAGKPTTQPEGDFNGSEKGFAPTSTGDPFFGRLRADMNFQFPEIAKAQS
                     IPVGCIHLQPDGRIQAFTFDPPIGKKGDDDLQTAAEAALSELKKARNANPEAVPSHLL
                     EITKQWLCFKFSVQSG"
     gene            complement(24169..24636)
                     /locus_tag="VFV99_21330"
     CDS             complement(24169..24636)
                     /locus_tag="VFV99_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5061931.1"
                     /translation="MAMSAGGGDNELNSEINVTPLVDVMLVLLIIFMITAPLLNNTGV
                     TLELPQVAAQNIEDPEGKLVMSIKADNTIYLGGTQITWAKLDAMLKANDRVQKESSLY
                     IEADTHLQYGVVITAMAIAKNAGVQHVMFLTDPSAQLDLSKLDQGQLVPGASK"
     gene            complement(24643..25383)
                     /locus_tag="VFV99_21335"
     CDS             complement(24643..25383)
                     /locus_tag="VFV99_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5061932.1"
                     /translation="MPGVSSTHVSTTLAAKLDIPHLFEQAHWIVQVVMILLAVMFVVG
                     LYIIIYKRLYIGRASAESNRFIEAFWKSRDIEQIYKQAQALRNSPISQMFVAGYTELA
                     KLHSDEQLKDDREGNLANIERALRRSMTHETTKLESMVPFLATTGSAAPFIGLFGTVM
                     GILFAFMDLSTPAAGADPAKKAAAATLDKVGPHIAEALFATAIGLVAAIPAVMAFNYF
                     MRRIKVLRAEMDTFSQDYLNIIKRHFLR"
     gene            25444..25902
                     /locus_tag="VFV99_21340"
     CDS             25444..25902
                     /locus_tag="VFV99_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061933.1"
                     /translation="MAKQLRAVVFVLCLGVHGCTQVDGGAVELSWKLRAESGSQNTFL
                     DCITHLQPTGQLVEVAKIRLEWDVDGATGTRSWECDDDHGVTNFELPEGRALLHVSPI
                     CANDMVADANTFRSPAPEQRNVIVGDTVSLGGIELLLEVSSCDLQACICQ"
     gene            26021..26761
                     /locus_tag="VFV99_21345"
     CDS             26021..26761
                     /locus_tag="VFV99_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061934.1"
                     /translation="MKKLVLASLIALAASQAAGCIITSGDDDTGENAFVSATWKLRSE
                     KTNTDVPCPPTFDTAALFNQPIDANGNNAGPVIVDLFNCADTAGTSAALPPTTYLTWV
                     EIQNSNGSNTYAKSLSAVVDVTVSDKTFNTQILVDGGYFQLQWQLQGASTNAALTCAQ
                     ANAAGGVETVGTDVSDPNNSNSDIFDCEDQYGVTAGYTMGTYTVSVAALNSSDASVGT
                     APALTNKVIQDKNQVTNLGTITIPITGM"
     gene            26873..27586
                     /locus_tag="VFV99_21350"
     CDS             26873..27586
                     /locus_tag="VFV99_21350"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5061935.1"
                     /translation="MDWSQLRITRGALMILGIQVALSLIWLMGNGELRVDIAEWVVAS
                     PSQVFEHYKVWTLVTSPLLEMGFLQLLLTGLMMWMLVPTLERFWGTPRFLRFVAITSV
                     AGTIAGCLVGWAIGSDVAITGFSPMIIGAVVAFGVIYARQPVQFFGVLPLSGREFMFG
                     LLAVFALSVLLQGHWEAGAAQGAAALAAAIMTSKRLSPGLAWKRWRIARARARLSVLE
                     GGAKAAKPKPKSDEQRWLN"
     gene            27623..29104
                     /locus_tag="VFV99_21355"
     CDS             27623..29104
                     /locus_tag="VFV99_21355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5061936.1"
                     /translation="MDEGDPQFESGTGLTTLFEGEWATVRRLRKGKLVVVSGQDQGKE
                     IEISKPRVTGGRSIISDLVVQDKAVSGTHFEVSARDDGYRLRDLNSRNGIFVGDLRVR
                     EAYLRPGTVFRIGHTNIQFQSMQDVVEIELSKKDRFDMMLGASPAMREIFAHLEKVAP
                     SELTCLITGETGTGKEMVARALHNASARKNKPFVVLDCGSIPRELIESTLFGHEKGSF
                     TGAVAQHTGCFEQANGGTIFLDEIGELDISLQPKLLRVLEQREIKRVGGDRTYKVDVR
                     VLAATNRDLREEVNRGTFREDLYFRLSVVHVELPAMRERREDIPGLANHFLRDVASRR
                     GMTMSFSQDAMAAMMSHSWPGNVREMRNVVERAAALSDGPVITRADLVFGREMGPSVV
                     VSHDLAQAGAQAAKRAAAELSGVELPQQSGPAIFDASLLKAGLGFKQAKQTVVDSFEI
                     AYLQALMVRNDGNITRSAQEAGLTRYHLRELLKRHGLKGGDVE"
     gene            29108..30070
                     /locus_tag="VFV99_21360"
     CDS             29108..30070
                     /locus_tag="VFV99_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061937.1"
                     /translation="MPRAVLDPALSVRVLGMRELRYQDTPALGDDRPQHVRAASGLAM
                     VGGRLAVIQDDCAFIGLVTDRVSALALPRGPGGRRRFEEQIGNKGDKLDLEACIVVKG
                     PLDEELWAFGSGSTPMREKIAVVGFSTRLHDGAPLYRRLREELSGPTAAESFRVPPVV
                     NIEGACVVGKQLWLFHRGNTGPHDAGPTVFRIDQPGFARWLASEGALPEIDYAQAYDL
                     GTANGVRLGFTDAVAHGATVFYLAVAEASPNAIDDGPVLASHIGIIQGDYVRACELVI
                     DGAPAKAEGLAFDPSDRNHAWVSIDPDDVDQPARLYELELVGPW"
     gene            complement(30071..>30568)
                     /locus_tag="VFV99_21365"
     CDS             complement(30071..>30568)
                     /locus_tag="VFV99_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061938.1"
                     /translation="NLGIIDELRIRTQLNIRPYLAGPKMIERALAKHYRRGFGRQIRR
                     PGEVPLDTAGDTVSLGLGPSGPRPDEAEIEAARRREGLRPTPKKTRPPPLEVPTAPSP
                     PAIGELVGPPPLVNRDAEIDALQDRISNLEALVARDEDVLRKLLSLLVEKGVATREEI
                     LEAIR"
BASE COUNT         5218 a         9971 c        10157 g         5222 t
ORIGIN      
        1 tcaccagatc gccaagtgtt gtccgcatga aagcggtagc tttgcagtcc gcgttccatt
       61 tcccgcatac gctgcgcgaa cagatgtgca ggtgcaggga ttgcgaccgc tccgctagct
      121 atctccatcg cttagcgcgg cacgaccgct gctctatacg tgtgcatgaa gcgcgcgaac
      181 cgagcatcga tgcctcactg gcgttcgccc ctgaaccaat tgcgctcgaa cctacattgg
      241 ctcgcgctcc taacggtcgc ctgcggttcg tcgccgccgc gtactgaaac tcccggatca
      301 cgcgggccgc atgccaacga gcacctcgcg gccgcgcgcg agcacgatga tgcggcgcgc
      361 gaacggtcga tgtatcccga tactcgcatc accgacggca ccggacgcgt cgaccagatg
      421 ttgattgcca ccccgtggca tcgcacgtgg gataccgccg gcgatcagga gcgtgcggca
      481 gccgcacatc gcagtgaagc gcaggcgatc tatgccgcgt atgacgaagc ctgcgccggc
      541 aggtcgagta gcgacatctc ggtctccccg atcgttcgct atggccaggg tggcggtgtt
      601 acgcccgatg gtgtcgagct gtatctgtcg accgcggcag ggccgccgga gcgcttgctc
      661 gcggatctca agtgtcaccg cgcgtggatg aggctggcac ccgcgaacat ggacgcatgc
      721 ccgctcgacc tcgcaggtct gttgatcgat gcgagaggca gcggcgaggg catcacgctc
      781 acgctcagcg tccgcgatcg ttcactgatc ccggagctcc agcgccgtgc cgcgcacgac
      841 ctcgagatcg ggcaccacca ctaattcagc tgcgcggtgg catcgagtaa cgcgccggcg
      901 aacgttgccg tcatcgcgag ggcaacgtcg gtgtgatctt cgaggacatc gaagattgcc
      961 ggtgctccgc tgcgcagtgc acggacgggc tgggttgttt cgatcgttgc gcggtgaggg
     1021 aggccggcga gtgtctcgag atgtccgatt gcggcgccgg gctcgaatag ctgttcgcca
     1081 tcgtcgcgaa gcgcacgcgc cccaccgtcg atgatgatca ggccaccgct cgcgggctct
     1141 cccgcgtggg cgatgacggc gcccgcccgc cactgtactt cctccgatgc atgcgcgagc
     1201 gtcgccagtg cctgcagccg cgccgacgtg aatggcaaca gctggcgcag cacgatgagg
     1261 cgctcgacaa ggccgagcgg tcccgcggcg caaccacacg tacgtgccgg cggcggcgca
     1321 gctgcgagca ggcgtcctgc cagctcgcgc acggtggcga gcagcacccc gaagttgtcc
     1381 tcgagcacct cgcccacctc gccggctgga agatacagcg cctggacgtc actggccgcg
     1441 acggcggagt gcgcggccgc gcgatgcgcc agtacctcga gcgcaccgaa cacctggtgt
     1501 gcggaccatg tctggccatg cggctgcgat tcgatgcgac cggcgaggat caggtgcacg
     1561 ccgtgtacgc gatcgcccgc cgccacgatc tgcgtgcccg caggaatcgt gatctcgacg
     1621 aggttttccg cgatcgtggc gagctcgtcg aggtcgatcg tttcgaacag cggaaactgc
     1681 cgcaactgca gcatgcgacg tacgccgcgt ggattggcgt cagacatcga gcctcgcgat
     1741 cgcctggtcg aaggcgtaaa tgacgagcgg cggaccgacc atcggtcgca accgggtcaa
     1801 atcgtggcgg agcgcgacca ggtggcgctc ggcgacatga tgagcgacga cgcacttgag
     1861 cgattcactg gagtcggcaa gtagcgcgcc gagcagcgcc tcgtacgtcg ggaacggacc
     1921 gactgcgagg tcaccgagct gcgaccggcg ttgctcggga gttagctcgt cgatgaccgc
     1981 gagcagcgcg ccgcgctggt ctaccggcag cagcgactcg acgatctcgc gcgcagcact
     2041 gcgacggttg ccgtcgcctc ggttgatcgc gtcgtgcacg ctgcgcaggg cgtggcgtgg
     2101 gtgcaggatg ccgagcgcgc ggaacgtgtg ttcgatcgcc caccgccgct tctcaccgag
     2161 tagctcgcga atcagctcgg cgccgggaga ggtgtcgccg gcgaccacga aagcgtcgag
     2221 gagcgttgtg tagcgcgctg catctgcgat cgaacggcgc acgtactcgt gcaggatcgc
     2281 gggatcgatc ggtagcaatg ggttgttcgc ccgcatccgg ccgagtgcgc gcaggatctt
     2341 gaactcggtc gtgccatcgg gctcgcgcaa gagacgagca acgagcgcgc tggcagccgc
     2401 gcgcgagcgg aaccgactga tcgtgcgcgg tacgtgacgc cgcacgccaa tcggcgtacg
     2461 cgcatcgtcg agcgcggcca tgagcttgtc gaggccacgc gaacccgttg cagtgaacac
     2521 gcggcggacg tcgccgcgca cgtgcggatc ctcgagcagt ggcacgatgc gatcgagatc
     2581 cacgagcgac ggtgcacgtg cgagcacacg cagcacttcg cgcatcacgg gaacctccct
     2641 cagcgcgagc agctgataga gcaggcgtcg aaaccgatcg tgcggcgtat agccgatcgc
     2701 gtgcgcgagc gcgatgcgat cggcaagtga gccgttcagc agcgcggcga cgccgctctc
     2761 gaccagtacc tcgcagtcgg catggtcgcc tagtcccacg agtgccgcgg cgcgcacgtc
     2821 gggattggga tcagcggcag ctgcctcgag ctgggcgcgg ttcgaatgcg tacggctcgc
     2881 tgcgatcagc gcagctgcgc ggattcttgg atcggagtgt tcgatcagat ggcgcagcac
     2941 acgtgcgaca tctgctcgca gctggccctc gagcagcagc agcgcgcgcc gcaccacgcc
     3001 ctcgcgcggg tgatagagca cgagcgcggg cacgcggccg ccgcgagcga gcagctcgag
     3061 tgcggcgagt gcttccggct cgtcgggact cgcgagcgcc tcggtgagca gtgcggccga
     3121 gtcaccgtcg agagcgggcg cacgaacgtc gcgctcgatc gcgccggctc gcaaggtgtc
     3181 ggcaaactgc gcgatgtagg cgcgctttgc gatccggacg gcgagcagcc acgcaacgct
     3241 ggtcgccgcg acgacgaagc cgagtagctg cgtgccgatt gcgagcgaag cgatcaggaa
     3301 ccacagcagg gccgcgatcg cttgtccgcc tcgctggcta atcgcgtcgg cgatcggttt
     3361 gccgccgtcg cgaacgagcg aaggcagtgg caggtacagg atctcgctcg cgacgcgctg
     3421 caggctgtgg cgcagaccgc catcgcagag cttgagcgcg atgatcgcga gcaacgtacc
     3481 ggtgagcgcg aacccgctgg cggtcgcgat gagcaagatc ggcagcacca tcagcgcgct
     3541 gccgacgccc caccgcgcga gcagtcgcgg cgtgacgacg agcaggatca gaaggccgac
     3601 gaagttgagc acggtgtaga tcgcgccgaa cgcggttgcg agatcggcgg ctgcgaagcg
     3661 ctcggcgagc acgcgcttga acgtcaggtc ggcgagcgtc aatgcgatcg tcgagatcaa
     3721 gccgacggcc acgaggagcc ggacgtagcg gcgcgaacga cgcgagagcg cttcgacacg
     3781 cttgtgtact ggcggtagcg tgtcgtcgag cgtgcgcggg gcgtagacga acgaggcgag
     3841 caatgtgagc gtgaacgcga tcgcgcccgc ggtcaccaga taatgcggcg ggacgatgcg
     3901 gccgagcagg ccagcgagcg cggagccggt catggcaccg aggacaccgc cggctccgat
     3961 cacggcgaac gcgcgcttcg cttccgcgat ctgcaggctg cgatcgatca gcgtccagaa
     4021 gctcggcacg acgagcgtcg ccacgaggcc cgtccacaca tagagcacga acacgaacgg
     4081 cggcgcgatc gggatcaaca cgccgataag taatgtgccg gcgatcgcgc cgacgagaaa
     4141 cgtgagcagc atccgccgcg ggtcacgcac gccgccccag cgccgcaccg cgacaaccgc
     4201 gagcatcgca accgccgcga tcgcgaggta tgcggatgcg agcgcgtgcg ggccgagccg
     4261 agcaagaaac agcgcgtcgc gcgcggtctc gagcaatgcg tgcgcgagca tgatcccgaa
     4321 caacagcgca gcgggaacga ctgcgatgcg ccgcatcttg gtcatgggcg ccagactccg
     4381 atcacgcgcg cagcctgcgt cgtcggatcg cgcgcgagat gaaagtaggt cgtgcccgtg
     4441 aactgcggcc gcgttgtatc gacgcgcacg atcgtatagc cgttgcgatc ggcggcaagc
     4501 ccgagacgcg tacaggtcac gccgctgtcg ccggccggtg catcggtcgc cgcgagctgc
     4561 gtgcccgcgt ggtcgtaggt cgtgatcgcg tagtgcgtat taccagcggg cgcgaacgcg
     4621 tgcgcgatcg cgaggtcctt gaagcacagc gtgtcgtcgg cgatcgccgg ctcgtcgagc
     4681 ggattgacgc gttcgaacca gtagcgagcc gtggcccgtt gtctcgcgat caatgcgtcg
     4741 acgagccatg ccgccgcctt tggatcgctg agctgcgcgc tgtcgacggc cgccttgatc
     4801 tggtcgcgcg tgaagcggat caagatcttc gacgcccaga acttgtcgat ccgatccgcg
     4861 acgtagatcg gcacgtaggc cgccgagctc gacacccacg cgcctgggtc gtagagatcc
     4921 gtttcgtaga gtccgagccc ccgcacgtcg cgccacttgc gttgttccca cggtcgctcg
     4981 agcgcgccga acgagagcaa cgagcgcgcc atcgcggccg gatcccagta gaactcgtac
     5041 ccgtaccggg cgtcgacatt cttcttcgcc gagaacccaa gtgcgacgcc gtagtcgacc
     5101 cagtagtgct tcacgtagtg ccgcttcgga tcggccggat cgccaaccca cacgtcgagc
     5161 atgttgcctt cgtgaatgtc gaagtgatcg agccagctga acacggcgta cgcaccgcgc
     5221 aggtcacggc gaagctcgtg cgggatacga tcgtttggat cgtcgccgcg cacgccctcg
     5281 gctgcatggc caccgagcgg tttgcccggc agccaatgcg agacgagcac gcgaatcctg
     5341 ccgtcggcag cggtctcgat cagggcaagc cgcttgtcga gctcgcgctg gccgagaggt
     5401 gacttgtggc cgaagatgtc cttcaccacc gcatcgggcg cgagcacgag gtcctgcttg
     5461 tgcacgtaca ggacgtagtc ttcggtgacg ttgtagccga acgcccagag cagcttgccc
     5521 acgatcactt gcgtcgcggt ctcggcctcc ggaaggccgc gcggatcgaa cttgagcagg
     5581 aacttctcgc cgcgcgtgtc cttgatgatg aagccaacgc tcatcccgcc aaccttcgtg
     5641 ctgacgatcg tccacggctt gaacgcttcg gggctgccga cgccgccggg tgccgtggcg
     5701 atttcctccg gcgatacgtc gcgcacgccg atgcggttcg tgaaccaggt cgaattcgga
     5761 acctcgtcga gtgcgttgat gccgcgcgca cgctcgggtc ggtgcagctc gagcgcgcgc
     5821 gtgatccggc gctggaactg gccatcatag ttgtagagcc agcgcacgaa cgcacgtggc
     5881 accggctgcg ccgcgacgtt tgctcgatca tttaccgcgc gcgccggtgg cgcgtttgca
     5941 aagcgcacgg gtggcaccgt cggtgtcgtc gcacagctcg caagcacaag gagtgggagt
     6001 aggcgcatca tcggtcctcc acgcgaggtt cgatgtcgaa caccggatcg aatgcgaggt
     6061 tgaagaacac gccgccgtcg atcgaagacg cgacgctcac gctcgcgatg aagcgccgca
     6121 cgtcgtgaag ttgcaacgag aagccgtatc cgacgcgcag gtttgtcggt tcgatgtcgc
     6181 gcggcgacgg aaacacgcgc ccgacgtcga tgaagagact cgccatcgga tagttgccga
     6241 gatcccacat gtactcgagc gagccgactg ccgcgaatcg atcgcggaag cgatcgcgtg
     6301 ggtagccgcg cagcagcgtc ttgccgccga gctccggcag ctgggtgaat gcgacgcttt
     6361 gcgtgtcgcc ggtcacgccc tcggcgtaca gccgcgtcgc tagcgcacgc ggacccgcgc
     6421 cgatgttcag gaactgctgt gcttcaccgc catacctcca gtagtcgacg ccgccatcga
     6481 gggcatgtac tggaccggca tagacagcga gcagcgatcc cgtgtcgagc acatgatgcc
     6541 cgccgaacgg cgtcgaaacc ctgcggctgt cccaccgcag ctcgagctcg ctgtacagat
     6601 ttcgggtgcc cggccagccg gtgagcgagc ttgccgtata gatcatgtcg atcggcgggc
     6661 cgtcgctgct atcggcgtac gcgatgtcgg tcagtgctgc ggcggcgcgc acgtgcaggt
     6721 cggaaatcgc gcggatgtcg acgcgcgtcg acgcgcgaaa caactcctcg cggtgatacg
     6781 cgatcatcga gccttcggtg ttgccgatgc cgtagaacgc gtcagcaggc cgccgttcga
     6841 gctcgccccg cagctcgagc tgcgttcgct caccgaagcg ctcgcccgag cgcaggccga
     6901 cgcttgcttg cgctcggtac tcgccgcccg tgccgccgcg aagtgcgagg tgctcgcgct
     6961 cgccaaacag gttgcgatgc acgaagcgag cgccgacggt gagaccgtag ctttggtcga
     7021 gcacgagcgt cgggtagagg ccgtacgtct cggattcgtc gaagaatacg cgcttgaagc
     7081 gatcgaccag ctggaagcga tcgagcgccc acacactcgc gcgcaccggt gacagcgcga
     7141 cttcgaccgc aatgcgcgga acgaataacg caccttggcc gatgtcgcgc agcaccgagt
     7201 cgtggtcgcc gggatccgtt cggccgctct cggtgcctgg cacgggcgcg gtctcgacat
     7261 cgacagccga gggttcgtcg cgcggcgggg gtggatcgtc ggcgcgtgcg agccctgtcg
     7321 tgagcatgga caccgctatg acgacgcgct tcatgcgtgt gctccgggaa gagggtggag
     7381 gacaggtgga ggcggcggtt cggactcgcg actgatcagg cgatgacggc gtgcgatgcc
     7441 gcccgcgatc gcgagccaca ccgtgatcag aatgagattg acgagcgcga ggtcgcggcc
     7501 cgtgaacgcg agctcgtgaa tgccgatgcc gacgagcacc gcagacagcg tgtcgccggc
     7561 gcgcacgaag aacgtgtcga tggcggcttt cgccttgtac ttgacgacgc ggtcggtcgg
     7621 caggaacagc gcctgccgca ccgtgttttg cagcgagtag tcagtgccgt tctccgcgat
     7681 cttcgcggtg cgcacgagca tgagcccgcc gaccgtaccg atcatcgcgt atgcgccgaa
     7741 cgcgatcagc ggcagcgcga acagcgcgcg gcgcacgccg agcttgtcga tgatgcgcga
     7801 gacgaggaac gcctggatca ggaagctcgt cacgttcacc cagtagaaga agtcgctgta
     7861 gaacgccttg atgagctcgc ggcgctcggc gttgatcacg ctcgcatcga ctccgatgcg
     7921 cgcaggcacg agctccatcg cgtgctcgcg cgccgcgttc gacagcacgt attcacccgt
     7981 cgtgttcacg aggttcgcga cgagtaacag acacgcgatc agcagcaggt agcgatcgcg
     8041 gaggaccagc gcgaagccgc ctgcaccggt gatcggctcg accgaccgat gatcgcggcc
     8101 gtgccggttg ttcacgtgct cgatcacatt gaacagcgcg atactggcgc cgaggatcag
     8161 tgctgcgacg agcagcaacg tgaacgtgtt cgcgagcttt gcgatgcgcg ggccgaagat
     8221 cgcaccgagt gagccacccg ttgcgatgat cgcgaacagc cgcttgcctt gctcttcggt
     8281 gtagaggtcg ttcgcgtagg accagaactg cgcgatcagg aacacgctca cgaggccgag
     8341 ccagacgaag aacgctagac cgatcggtac accggctcgt ccgaggacga agaacgctag
     8401 cagcgatgcg atcacgatcg cgtaacagac gttgatcaaa cggatgcgcc gcgtgcgatt
     8461 cgccaacatg ccgtacgcag gcacgacgcc gacgagcagc agcgccatcg cgccgttcgc
     8521 ataggtcttg agctcgtcgc cgcgcagtcc ccacgtgccg ccggacagga tcagaccctc
     8581 gcgcgccgtc ttcatcacgt aataggacgt gaggatcaaa aagacgcaga cgagcatcaa
     8641 cgcagcaatt acgccctctc ccggccgcac cttgctgaat ggtcgcagca gccgatcgag
     8701 gaacgtcagc gatgcagtac ccgaggtcac gtagccgcga agtgcaacgg tggtgcctag
     8761 ctacgcgtgc gactacgagt gattgccagt ctcattcacg caacacgagc gttcggcaac
     8821 gagaattgcg cagagtctgc ggtgcaggtt tttcgtcgac aaccgcgggc aacctcgacg
     8881 aggatctcgt tgtctcgtcc ttgtcacgga tgcgacccgt gcttagctgt aactcgcaac
     8941 gggggagaca acgatgctgt atcaacgggt gatttcgatc tcgatcttgg gtcttgcggc
     9001 ctgtggcaat gtctcgggac acgtcagctc gacgaagact ggcgacacga cacatccggc
     9061 cgagcgcggc tcgccggtcg atgcgctgca tgccgcgtgc ggcgatggcg cggcaacgcc
     9121 gttcggcacg aacacggtca aacgacagcc gtatctgcaa caggtcacgg catcgggcgc
     9181 gatgctcggc tgggtgagca cgcagcccgc aggcgagcac gtcagcgtga cgaagccgcc
     9241 cgatgcgatg gcgacgctgg cgcctggtgt cgtcgaagac tacgcgctgc gcagcgctgg
     9301 cgagaaccag atgtggtcga cgctcgacaa gctcgagccg agctcgatct actgctacca
     9361 gctggccaac gacacgcaga cgctcaacgc gccgaccgga tttcgcacgg cgccggccgc
     9421 cgacgatcca gcccccgttc gcttcctcgc gttcggcgac tccggtggtg gtggctccga
     9481 tcagtacgcg ctgatggagc agatgtacac ggtgccgttc gatctcatga tccataccgg
     9541 cgacatcgcg tacgacgacg gcacgatcga acagtacgaa gacaacgtgt tcggcgtgta
     9601 cgccgatctg ttccgcagca ttccgttctt ccccgcgtcg ggaaatcacg actatcacac
     9661 gcaggcaggc gcgccgttcc gcgacgtgtt caacctgccc ggggccaacg gcgagaagtg
     9721 gtactcgtac gactggggcc gcgtgcactt cgtcgcgctc gataccgaag cggactacgc
     9781 gacgcagatg aagtggctcg agcaagatct cgcagcgtcg caggcgccgt ggaagatcgt
     9841 gtacatgcac aagccaccgt actcgtcggg catccacggc tcggatacga ggctacgcaa
     9901 catgctctcg ctcatcgcgg agaggggcgg cgttcagctg gtgctcgccg gtcacgatca
     9961 cgactacgag cgcatgaagc cgcagaacgg catcgcgtac gtcgtgaccg gtggcggcgg
    10021 tattggcacg cgtggcgtcg ggtcctcgag ctttaccgag ttctcggtcg acgtcatcca
    10081 cttcgtctac gtcgaagtcg gggtcgacga gctcacgctg cacgcgatcg acgcgacggg
    10141 caaagagttc gactcgatgg tcgtcccgcg cacgcgcgcc tgaccgctgc gcgcaggtgt
    10201 tgcgcgaaaa ccgatacatg agttggcgcg cgagatgctc tacctgcgga catgcggtgc
    10261 atgaatttgc tcgcagtcgg gttggcgctg tcggcatgta cttgcgagag cggacctaat
    10321 tcagcaggcg gcgtagcgac tcctcccgag agcacggcgc cgccactcgc cgttcctgcg
    10381 aacgcattga tgcgggcgca tatgacggtg cacctcgagg aggcggctgc gctgcaagcg
    10441 gccatcgcac ggggctggct cgtcgatgcg cgcgagcgtg cgcgctggtt cgcgtcgcac
    10501 gacatggaag cgccgccgtc ctggcagccg tacctcgatg acatgcggta tgcagcgatg
    10561 cgcatcgcca gggcgcgcga tgtcgcgacc gctggtgcgc agatcggcct gctcggacgc
    10621 gcatgcggtg cgtgtcacga ggcaaacgct gcaacgcctg cattccagta cgacccgccg
    10681 ccgaccgacg acgcgacgct cgaagcgcag atgcgtcgcc accagtgggc ggccgcgcga
    10741 atgtgggaag gcgttgtcgg gcccgcagac gcggcctggc aagacggcgc tcgcgtgatg
    10801 gcaacgacgc gcctcgatgt cgcgcgcacc gcgcacgaga agcccaatgc cgacgtcttc
    10861 gagcttgccg aacggctacg ggaccaagcg acacaagcac tcacgatgac cgcacgcgat
    10921 gcccgcgcgc ggttctacgg agaaatgatg gagacctgcg cgagctgtca cagcatcgtg
    10981 cggccgattg cgcgggccgc cgcgcgctga ggagaacacc atgaaagccg cccgcatcca
    11041 cgcctatcgc caaccgctca ctctcgacga agtgcagatg ccgatcgccc gccccggcga
    11101 ggtcatcgtc aaggtcgcag ggtcgggctt ctgtcatagc gacctgcacg tcatcgatgg
    11161 cgagctcccc gtcttgccgc gcatgccgct cacgctcggc catgaaaatg ctggttgggt
    11221 cgccgagacc ggcgctggcg tcagcggcgt tcgcgaaggc gatccggtcg tcgtctacgg
    11281 tggctggggc tgcggccgct gcgactactg cgtgacgggc cacgagcagt tgtgcggggc
    11341 acccgaatgg tgcggtctgt cgcaatggga tggcggttac gccgagtacc tgcgcgtgcc
    11401 gcacgagcgc tacctcgtga agctcacgaa gctcgacccg gtctgggccg cgccgctcgc
    11461 cgacgcggca ttgacgccgt atcgtgcggt gaagaaggcg ctgccactcc tcgagcccga
    11521 tcacgccgtc ctcgcgatcg gtctcggcgg tctcggccag tacggactca agctgttgcg
    11581 gctgctctcc ggcgcgccga tcatcgcggt cgatacgtcg atcgagaaac gccgcatcgc
    11641 ctacgagctc ggcgccgaga cggtgatcga cggcgcggct cccgacctcg tcgaccgcat
    11701 caacgctgcc acgcatggca acggcgtcac tgccgcgttc gacttcgtcg gcgccgagca
    11761 cacgctcgac accgcggtgc gcgcgacccg ggcgctcggc aaggtgttcc aggtcggcct
    11821 cggcggcggc acggcgcggc tcaaggtgct cgacaacgcg aagttcgaag tcggcttcga
    11881 gtgcacgctg tggggcacga tcaaggagct gcgcgaagtg gtcgcgctcg tcgaggatgg
    11941 tcgactcgcg ctcggccagg cggaaaccgc gccgctcgat cagatcaaca acgtctacgc
    12001 gcgcctcaaa cgcggtgacg tgcgcggacg cgtcatcatc acgccgtcgg cgtaactact
    12061 tttgcggatt gctcgcgtcg gcgctcttgt cgtgcaggtt gacgtcgacg gtgatgtgaa
    12121 tcggctcgac cttcaccggg tcgaccttca cttgctgagc tccgcacgcg gcgagcagca
    12181 cggcaacaac gatccacttc atggcacagc tcctggcaga cgcgccgcga catcgcgtgc
    12241 accacgaatc ccgatcgcga catcgagctc ctggtggttg cggcggccgc ggccgcgcgt
    12301 ggacaagcga agatcggtgc cggcacccgg cggtgcgagc tcggcggtca gcgtgtcgaa
    12361 ctggaagtcc gtcaccgcgt cgagaagctt cgcgtgcacc gcgaatggcg actgctcctt
    12421 cgtgagtcgg tcgcgccagg tacggtcggt gacctggagg ctgccgcctt tgcgggcatg
    12481 cagctcgccg ctgtcgagcg accagccgtc gcggtcgagc cgcagcgcga ccgtgccgtc
    12541 gacgagccca ctgcccgtaa ggtgctcggt cggctcgagg agctgggcga gctggagacc
    12601 gcgcgcgtgg acgacgacgt cgctgccggc gcgcacgatg aacggctcca cctcgacctc
    12661 gccgccaagg acgtgagcct tgccgctgac tgccagggtt cgctcggcgt cgactttcgc
    12721 ttcgagagat accgcccaac ctttcgggct cggatcgcgt acgtcgatcg tgatctcgag
    12781 cgctttgcca ctcgccgacg cggtgccggc catccgagtc ttggtgcctt gtacgtcgag
    12841 cgtcgagtcc gcgacctttg catgccgaaa tggcagcgcg gcgttcgcgg tgcgcgtcag
    12901 cgctggcgcg agtcgcgcga gtgcagccgt cgacacgctc gcgtgtgtga tcgagacgtc
    12961 gttcacgtca cgccatagca gcgacaagcc gcggtcgaag tcgagcgttc caagatcgag
    13021 accatcgtcc aggtggacat tgcgaagctg catgtgttgc aagccgatgc tcgcgacctc
    13081 gagccgcgca tgcggaaagc cccgctcgac gaggccggcg cgaatatggt gctcgacgac
    13141 gatcggcagc agcacgtaag caacgcctgc ggctgcagcg acccccatcg ctatcttcgt
    13201 acgacgacgc aacaccacgg ttgtgaccgt caccctaacc accgggttgc ttctgtaaac
    13261 agcgcagcga ttgcgcaatc tctcgacgaa ttcgtggccg ttactttgca gccagttgcc
    13321 gcatgcatcg aacctgcatc gccatcgtcg acgcctcgcg tgcccgcctg ttcactctcg
    13381 agcgctccgc ggaagacagc agaatccacg aggttcttac cgagcaacgc gacttggtga
    13441 accctggatt ggacgcgacg ttttcgcgac gaatcgtcga cgaggtcgct gcgctgatcc
    13501 gcgcagttca tgcggatcgc gtgattctct gcgcaagcgc gcgaatgctc ggcgagctgc
    13561 gcaatgccgg ctcatctcga ctagacgcgc gtgtcatcga cgagctgccg cgcgatctcg
    13621 tcaagctcac gcctgcgaag ctgcgtgacc agctcgcgtc gtacggcctt ctcccggccg
    13681 cgcagcgtgc agcagcgatg tgagcgccac gggtagcgcg ccggcgagcg cggcagcgac
    13741 gacgtgtggc atcccgagcg gcgcgaagcc gaacaacgtc ctcagtcctg ggatcaccag
    13801 cgtcagcgcg aacacgccgg tcaccgcgac gagcatccag aagatggcgg ggttcttgcc
    13861 gacgtgactg ccgcgggcga ggcgctcgcg cgaagcggcg acgagcatga ggtccgccgc
    13921 gatcagcgcg acgaacgcga gggcacgcac cgtgtcggca gcgtaacctg cgttgtgcgc
    13981 aaccgctgcc acggcgagtg gaccgagggc agctgccacg ccgagcagca gagcgaaccc
    14041 gacgcgcttg gcgtcgaaca gatgctcggt tcgcgcgcgt ggtttgcgct gcatgacatc
    14101 gcgggcgggt ggatcgagct cgaacacgat cgagcacgtc ggatcgatga cgagctcgag
    14161 cagcacgacg tggatcggac cgatcaaggg tggccagccg agcaacggcg gcagcaacgc
    14221 aacgccgacg atcggcacgt gcacggagag caagtagctc gcgaccttgc gcaggttgtc
    14281 gtagatcgtt cgcccggcgc ggatcgcggc ggcgatcgca gcgagcgtgt cgtcgaggat
    14341 cacgagcccc gatgcttcgc gcgcgacctc ggtgccgtga cccatcgcga tgccgacgtc
    14401 ggccgccttg agcgcgggtg cgtcgttgac gccgtcgccg gtcatgccga cgacctcgcc
    14461 ggcgaggcgt agtgcgtgga tgatgcgtag cttgtgggcc ggcgcggcgc gcgcgatcac
    14521 ttcggtgtgc tcgagcgcgt atgcgagcgc ttcctcgtcg agttgctcga gatccggccc
    14581 ggtcagcacc gacccggcgt cgagccctgc ggcgcgtgcg atcgcgcgag ccgtcgtcgg
    14641 gtgatcgccc gtgatcatca cgatgcgcac gccggcttcg tgacactgtt cgatcatctg
    14701 cttggtctcg ggccgaagcg ggtcggcgag cccgaccagt ccgaccagct cgaacgcgaa
    14761 gtcatgcggc tgtgtcggcg tcttggcacc gccgtgcgtg tggtaggcgc gcgcaacgcc
    14821 gagcacgcgt aggccttcac gcgccatcag atccgcttgc gctcgccagt acgccgcttg
    14881 ctccggcccc agatggcaaa ggtcgatgat cgcctccggc gcgcctttgg tggcgacgac
    14941 ggtccgttgc tcgtcatcac gccacacgtg cgtgaccgcg agcaggcccg gcgacagcgg
    15001 atactcgcgc acccagcgcc acgccggatg cacgtgctcg gtcttcgcga gcgcctggtc
    15061 ggcgagcctg acgaacgcgc ggtccatcgg atcgaccggc tgctgcgggc aggcgagggc
    15121 accgaactcg atcgcttcgt ggacggattc gggcagcttc gtcgcgtcga ccgcgatatt
    15181 cgcggagccc tccagcgaca tgacccggcg aatcgccatt tggttctgcg tgagcgttcc
    15241 ggtcttgtcg acgcacaaca ccgtcactgc gccgagcgtt tcgatcacgg cggcacggcg
    15301 ggcgagcacg tggtgcttcg cgattcgcca cgcgccgagc gtgaggaaga tcgtgagcac
    15361 gagcggcagc tcttccggca acaccgacat cgcgagcgtg atgcctgcga gcagtgcctc
    15421 gaggatgccg gcaccggtca cgatgtaaat cgcggcgagc agcacgcaga gtccgatcgc
    15481 gacgatcgcg actcgcgtaa cgatggtcgc cacctcgcga tgcaacggag ctctcgcgag
    15541 ctcgatccca ccgagcgcag caccgatctt gccgacctcg gtacgtggtc cggtcaacat
    15601 cacttccgcg acggcgttgc ccgatccgac gagcgtgccc gagaagaccg acgcaccttc
    15661 ctcgcccggt cgtccgagcg cgcgcacggc cggatcgggt cgatgcagca cgggcaccga
    15721 ctcaccggtc aacagcgatt cgtcgacggc gagcggggtg ccggcgcgca gcagcgcatc
    15781 cgctggaatg cggtcgccct cggaaaggtg aatgacatcc gcgggcacga gctcgcgcgc
    15841 gtcgacctcc tgccagcggc catcgcgata gacgcgcgca atcgggctcg ccagctcgcg
    15901 aagtgcatcg agtgcacgtt cggtgcggcg ttcctgtgcg agcgtgatcg cgacaatcgc
    15961 gagcaccgag acgccgagtg caccggcctc gagcgcatcg ccgaacgcga tgtagatggc
    16021 ggtcgcgacc agcagcaaca acaacattgg ttcgcgcaga actccaccga tgtgctggag
    16081 cagcgtacgg cgatctgcgg acggcatctc gttgcggccg accgcgcgtt gtcgggcggc
    16141 tacttcggcg gagctcaagc cggttacgct cgcaggcagc tccgcgtcca tacgatgagg
    16201 tggagcatgc ggcgtgccga ttattcgtcg tcgagagtac gcaggaatgc caggagatcc
    16261 gcgatgtcct gcttgctcag agtggtcccg gtgcgcacgc gttgcgcgtt ggcgacgact
    16321 tcgggcgcgg gggggttgca cgtgccggga cgcaggcacg ttcccgctag cagctcgatc
    16381 atctcgtcga gtcgcctcat gtagccgtta tggccgtacg gggatgtctt cgcgatgttg
    16441 cgcagcgttg gcacgcgaaa cagtccgtca tcgcgtgggt cgccggtcgt cttcgcgagg
    16501 ccgcgatcga tgaatgcagc accgtcggga ttgagcgcgg tcggtagcat gtagaacgga
    16561 ctgtcggcga agcggggcag accgatgttc gcgtagctga agttcgtgaa cagcggcggc
    16621 gaaccgtcgg ggcccggccg gctcggatga cagctcgcac agttgccgcg ggccggatcc
    16681 tcgaagatcg cgaggccgcg tgcttctgcc gcagcgagct cgacggtgcc ggcgaggtag
    16741 cgatcgtact tcgaggaaaa cggcgcgaac ccggacgtgc gctcgaacgc cgcgatcgca
    16801 tcgccaagat gcgcaagtgc tgtcgcctca tcggtcagcg catccttgcc gaacacgtcg
    16861 tggaactggc ggccgtaata gcgacggatc ttcgcgacca cggcgccgcg atcgggattg
    16921 ttcatctcga gtggattcgt caatgggctg agcgcctgcg tctcgagcga gtcggcgcgg
    16981 ccgtcccaga acaggccacc gaccatgctc tgcgtgtgcg cgtcgcgatg cagcgggggc
    17041 acgaagctcg cgtacatcgc ggtcggagca ttccgaacgc cgaaccgttc gcgaatcacg
    17101 cccgccgaga cgcgatcgtt ctccggatcg gtgaacgcga gcttggcgtc gtggcagtcg
    17161 gcgcatgatt gaccggcagg ctcgctgagt cgggtatcgt tgaacagttg ctcgccgagc
    17221 tctaccttcg tgatcggctt cccgggcgcc gcgttagcgc agccgacgag accgagcgca
    17281 atgagcgacc gcatggcgcc atcaatgtgg gttgccgacg ttgcatcgca attcgcaaaa
    17341 gctgcggcta gagcacggtt cgcacgacga gcacgttccg gtcggcgtgg ttcacgacct
    17401 tcgcggcggt cgtgccgatc agtcgatcga ggccgctgta gccgtgcgag ccgatcacga
    17461 tcagatcggc gtcgacctct ttcgcttcac gcacgatggc atcccacgcc gtgccgacgc
    17521 cggtcgtgat ggattcgatc agcgccggcg agaccgtgct cgcgatgcga tcgagcgatg
    17581 ccagcgccgt cgaacggagt gtctcctcga gccggatcgg cggtagcgag atcagatcgg
    17641 ctggtagctc tggcggcacg ccgaccgcgc gaaacagcac gagcttcgca tgtgtcaggt
    17701 tcgcgaggcg cgctgccgca tcgagcacga cttgcgcgcg gggtgaacca tcgacggcta
    17761 ccaggatgcg tttcatttct tgccacgaac ggtcaggacc gggaccgtgg acgtacgaac
    17821 aaccgtttca gcgacgctgc cgagcaacgc gcgcgagatc ccgcgccggc cgtgagtgcc
    17881 cattacgatc aaatctgcct tgagctcctc cgcggtctgc aggatcacgt cgcgcgcgtc
    17941 gccggcacga acatgggttt gggcggtcag cttgcggtcg cgcacgagct gatcgagcgc
    18001 ggcctgattg tcggtgatca tcgtgtcgat catcgtcgcg gtgacgccga cgccgagctc
    18061 gggaacgccg tacgacggca caccgatcac cgtcagcagg tgaaggcgag cgtcgagctt
    18121 cggcgcgagc tcgcgcgcat agtcgagcgc ggtctccgac gtcgaggaaa aatcgatcgg
    18181 cacgagaatg gtcgaaatct tcatggcctg tcctccacgg ttcggcgcaa tagatcgccg
    18241 agtgcacgca acgcgtcagt aacggtgaag atgccttcga cacccttgag acccagcacg
    18301 acgaccgagc tgcagtgctt tcgctccatc aggtcgatga cctcatccat cggcgtatcc
    18361 gccgagacgt gcgcgacgtc ttccgtcatc agctccccga ccgtcatgtc gtgaagcgga
    18421 tgcacgggat ggagatcgcg atcgctcacg atgccgacga gctcgtcgtg ctccatcacg
    18481 ggtaagtggt ggatgtcgcg cgtcgccatc aggtgccggg ccgacgacag cttctcgtgc
    18541 gggctgatcg cgtacggatg gttcgtcatg tagcgagaaa ccggcggcat cagcatgcaa
    18601 acctgcagtg cagcgcgcgt gcccgcacaa atgcgcgagc cgtcgctcag ctaggtgcaa
    18661 cccctgcgca gtgctcggag aaccacgcag cacgtgcgcg cccggcctcg caggaacgcg
    18721 aggtttgccc ggaacgagac ttgctctcgt aaggagcatg aatccggtct tgctcgtgtc
    18781 cttcgcggcg ctgccctggc tacagggctt ctttgcgccg acgagcgaga ccgagcaagg
    18841 ctcgcgcgac gtgctcgccg agctggcgga gtggcatgag cccgtcgacg actgcagcgc
    18901 cagcgcgtat ggcgggctcg agatgcgcgc cgacgtcagc gcgagcgaag gtcaggaacg
    18961 cgttctcgcg tcgtacacgc aaggcgtatt cgtattcgat cgcgaccagc acttgctcgc
    19021 ccaggcgccc gccttcgcat gcgctggctc gagtgacgag ctggtcgcga tggcagccgg
    19081 tgacgcatcg atcggcacgc cagtcatcgc gctcgctgcg accaccggcg gtcgcgcgac
    19141 gaacgtgacc tggctgacgc tctatcgggt tgccggcagt ggcgagctgc agcctgtatt
    19201 caacggcgaa gtcgagcgcc acgatggcga caccgcgcgc acgggcgtcg tcaccttgat
    19261 ccccggcggc ctcatctatc gcgacacccg cggctcgatc gggctctggc tgtacagcac
    19321 cgagcttggt cgctacgtcg agctgtttac cagccagccg aacgtgtgag cgtcggcgct
    19381 cacccggctt tacgaagcgg ggtgcgaatc ttggcgtcga tctcgagggg cgtcttgagc
    19441 gcgttcgcga tcagacaatg ttgcttggtc tcgtcgagta ccgcgcgcgc tcgctcgaca
    19501 tcgctcactt ccatatcgat gtcgacggtg aatttcgtga acgcgaggcc gtgctcgctg
    19561 cgatccacga cgccgccaac gcgcgcttcc catgcgagca ggtccacccg cgcacgggca
    19621 gcgagcgcct cgaacgtcgt gaggacgctc atgccgatcg aggacagcag cagttgctcg
    19681 gcggaccacg cggtcgcgtc gccgtcgaat tccggcggtg gtcctgcgga gatgagcggg
    19741 cggggcgggg cttcgagtcg tgcccgcgag ctcagcgtcc tcacgagcca tgtctggtag
    19801 tgatgaggaa acggaacggg catgcagcgc agcggagcat gttccgttcc atggccggac
    19861 gtgcggcact gtcacgtgtg cgcgccgacg cggtggcacg acgcttgaag ccatggggct
    19921 gcatggcttc tcctcactcc gctgacactc tgccggcagc tcctcgtcgg agatcgtggt
    19981 ggacgtggaa cctcggccgc atcgctggga tcccgatccg catgcatctg acgctgctca
    20041 tcttgctcgt gtggatcgcg agcacgtacc tgctgcacgg cgccaatctc agcacgacgg
    20101 cgttcggtct cgtcctggtg ctgacgatct tcgcgatcat cgtcgttcac gagctcggtc
    20161 acgcgctcgt tgctcgtcgc tatggaatcg cgacgcgcga catcatgttg ttgccggtcg
    20221 gtggcatctc gagcttggaa cgaatgccag aaaaaccaac gcaggagctt gcggtcgcga
    20281 tcgccggccc gctgatcaac gtcgtgctcg cgggcctctt gtacatcggc atcaagatca
    20341 ccggcggcag cacgcacatc acggagccga tgtcgatcgg cgccgcgttc gcaacccagc
    20401 tgatgtggat caacatcggc ctcgcggtgt tcaacctgct acccgcgttt ccgatggacg
    20461 gcggccgtgt ggcgcgctcg ctcctcgcga tgcgcatggg gtacgaccgc gcgaccgata
    20521 tcgcagcgca gctcggcaag ttcttcgcga tcctgatcgg cttcttcggc ctcttctaca
    20581 gcccgatcct catcttgatc gcgttcgtgg tgtggtccgg tgcgtcgcaa gagcgcgcgc
    20641 tcgtccacct caagaccgcg atcagcggcg tgcccgtgag tgccgcgatg ctccgccgcg
    20701 tcgacagcgt ctcgcccgag cagccgctcg aagatgccgc cgcgatgctc ttgcgcggtg
    20761 gcctcaacca gctgccggtg atcgatcacg gcgaaccggt cggcgtgatc acgcgcagcg
    20821 acgtcgcgac tgcgctctcg catgccggtc ccgacgccac cgtcgcgaat gcgccacagc
    20881 atgcgatcgt gatggtgtcg ccggccgact cgctcgactc ggtgctcgac aagctgcgcc
    20941 aatcgccgga ttccgtcgcg ctcgtcgtcg atcacggcca cccggtcggt gtgctcaccg
    21001 ccgagcatct cgccgcgtac gtcgcgctgc actcgcagcg tcgcgctgcg tgatcacatc
    21061 gctcacatca tcgtcggctg cggcgacacc ttgccggtga gcgactgccc cgacagcacg
    21121 ctgatcacgc cgagcccgcc accgccggcg ccggcgcccg ccccggtgcc atcgttgccg
    21181 ccattggcgg caggcttgcc ttggatcgca ccgtcgccac cgcagccagg cgtcgcgccg
    21241 gcacagctcg tcgttccgcc gccaaagccg ccgggcgcgg ccttgttgta ctcggtcgtc
    21301 tccccgccgg gattcccgct ggcggcgttg tccgaaccgc cgccaccacc gccgccgttc
    21361 gccatcaacg cgccgttcat gccgaggacg atcgtggccg catgcagcag gatcattccg
    21421 ccgctgccgc caccgccgcc gccacccttc gacgccgaac cgccaccggc gccaccacct
    21481 gatgcgttga cgacgcccgc gatcatgagc gacccgccgg cgagcaagta gacgccacca
    21541 ccgccgtttc ctgcggcgcc ttccagcgac gcgccaccgc cacggccacc gcgcccgccg
    21601 gcacaaccgc cgtgcagcgt gttcgcggcg aacgagccgc cgaccggtgc tgcgccctgc
    21661 gtctgcccca tatctcctgc gccgccgtga ccaccgagcg agccaaagct gccgccggct
    21721 ccgccgccac caccgttcgt gctggcggtg ccatcgggca gcatcgggca cgaggctgca
    21781 ttggcacctg cgccgactgt gccaccgtgg cttgccacgt cgagcgtgcc gtcgatcgag
    21841 atgtcgccga ccgcaaacac cacgatcggc agcgcaccga cggcagtgaa cgaagtgtcg
    21901 acggtcacgc gcgagcccgc gagcacgcac accatcgcgc cattgacgtc cttgcgcagc
    21961 ggacagtcct gcgtggtgtc gagcgtgccg tcgaggttca gtatcatcgt cggtgcgctg
    22021 tcgacgcaga tctcgagctt gccgctaccg aggcacagcg gtgcgccatc gatggtgcgc
    22081 gcgtcggtcg tgtgctccgg ctcatcgagc ccgaaccact gactacatcc cgccagcgcg
    22141 acgacaacca gcgcccgcat gtcgccacga tatcacctcg tggcgcccgt tcctcagtct
    22201 ttcggcgccg ggccccagtc gacgcccgtt gtcgagccgc tgtagacctt cacggcctta
    22261 ccggttccgt cggcgttcgc gatgaaaata ccctgacctg cgcgcgagaa cgcgatcgca
    22321 cggccgttcg gcgagaacga cggctcttcg ttgctgcctt cgttctgcgt gatgcgcgtc
    22381 atcgccttgc tatcgagatc gagcgcgacg atgtcgtact tgccgccgtc acgcgtcgtg
    22441 tacgcgatga tccgcttgcc cggtttcggc gaccacgtcg gcgtcgtgtt gtagctgccg
    22501 ttcgtcgaca cctgcttcgc cgcgccaccg ctcgccggca tcacgaagat ctgcggacca
    22561 ccgttgcgat cgctgacgaa cgcgatctcg gcgccgttcg gcgaccacgc tggcgacgtg
    22621 tcgatcgcct tgtccttcgt gagccgcgag atgaccgagc cgtcgctcgc gttgatgatg
    22681 tagatctccg ggttgccgtc cttcgacagc gtcaccgcga tcttgctgcc atccggcgac
    22741 cacgacgcgc cggtgttcat gccgcgctgc ttgctgagct gcttcggccg gccaccaccg
    22801 gcaggcgcga cgtaaagatc ggggttcgcg cgcatgaagc tcgtgtacgc gatctggccg
    22861 cccgacggcg accacgcggg caagatgttc gtcgagctgt tgttcgacac cttgtagacg
    22921 ccgttaccgt cgaaatccat cgccaggatc gcgctggtgc ctttgccctt ctgcgtgaac
    22981 gcgatcttcg agccgaagaa gccgggctcg ccggtgtagt acttcacgac ctcgttgcac
    23041 cagcgatgca cgatcgtgcg cgtgtcagtg ccggcgcgct tgtacgtctt ctcgagcgtg
    23101 ggcttgttgc ccttgctgac ctcgtagagc ttgaagtcga tcgcgtcgcc ggtcgccttg
    23161 tacttgatga cgccgtacgc gccgacgtct ttccacttct gcggatcgat gccggtaccc
    23221 tcgctcttga gatcggcgag gaacccctgc gggtcgagta ccttgaacgg gcccgcgacg
    23281 ttgagatcga agctctcgac ctgcgagatc tccttcgccg tcgcatcgcc ttcaggcgcc
    23341 gtcggcagcg cgatcggata tttgccgccc ttcgtgcccg aaacgtcgat cactgcgccg
    23401 ccaccaccgg ctggctgcgc ctgcaacgcg gacgcgccga acacaatggc agcgacgccg
    23461 actatcccga ttgaacgctg aatttgaagc atagccattg tttcgtgatc tcgaggaggt
    23521 gagacggaac tgcctcgggg tttgcgttgc gcgccttctt cagctcgctg agcgcagctt
    23581 ccgctgccgt ttgtagatcg tcgtcgccct tcttgccgat cggtggatcg aacgtgaacg
    23641 cttgaatcct gccatcaggc tgaaggtgaa tgcagcccac tgggatgctc tgtgctttgg
    23701 cgatctcggg gaactgaaaa ttcatgtcgg cgcgcaaacg gccgaaaaat gggtcacccg
    23761 tgctggtggg cgcgaacccc ttttcgctgc cgttgaagtc gccttcaggc tgcgtcgtgg
    23821 gcttacccgc ttcgccgtcg ttgcgatgga tgcctttgaa cggatcgttt ggatcgatct
    23881 tgggatcctt cttcttgtcg tccttcttct cttcgacctt cttcttttcg tcgtgcgaga
    23941 cgcccaccgg cttcacgtcc tcgacttggt cgaatttttt ctgcggctgc ttcgtcggag
    24001 atttcttgta cgcgatcgtt gcctcgagca cctgacggtc gtcgagatac ttcggcgggt
    24061 gcacggcggc cgccgagaag tagacgaccg gcgcgatcag cgacgcgctc aagatcgccg
    24121 cgccgatgag cgaaaccgcc tgcatctcgc cgtcgttgag cgacatatct acttagacgc
    24181 tccaggcacg agctggcctt ggtcgagctt cgaaagatcg agctgagccg acggatcagt
    24241 aagaaacatc acgtgttgga cgccggcgtt cttggcgatc gccatggcgg tgatcacgac
    24301 gccgtactgc agatgcgtat cggcctcgat gtagagcgag ctttccttct gcacgcggtc
    24361 gttcgccttc agcatcgcgt cgagcttcgc ccacgtgatc tgcgtgccgc cgagatagat
    24421 cgtgttgtcg gccttgatcg acatcacgag cttgccctcg ggatcttcga tgttctgcgc
    24481 ggcgacctgc ggcagctcga gcgtgacgcc cgtgttgttg agcagcggcg ccgtgatcat
    24541 gaagatgatc aacagcacga gcatgacgtc gacgagcggc gtcacgttga tctcgctatt
    24601 gagctcgtta tcgccgccgc ctgctgacat cgccatggcg cgttacctca ggaagtgccg
    24661 cttgatgatg ttgagatagt cctgcgagaa cgtatccatc tcggcgcgca gcaccttgat
    24721 ccggcgcatg aagtagttga acgccatcac ggcggggatc gcggcgacga gaccgatcgc
    24781 agtcgcaaac agtgcttcgg cgatgtgcgg gccgaccttg tccagcgtgg ccgcggcggc
    24841 cttcttcgcc ggatcggcac cagcagcagg cgtcgacaga tccatgaacg cgaacaagat
    24901 gcccatcacg gtgccgaaca ggccgatgaa cggcgccgcc gaaccggtgg tcgcgaggaa
    24961 cggcaccatg ctctcgagct ttgtcgtctc gtgcgtcatc gatcggcgca gcgcgcgttc
    25021 gatgttggcg aggttgcctt cgcgatcgtc cttgagctgc tcgtctgaat gcagcttcgc
    25081 gagctcggtg tagcccgcga cgaacatctg cgagatggga ctgttccgca gcgcctgcgc
    25141 ttgcttgtag atttgctcga tgtcgcgtga cttccagaac gcttctatga agcggttact
    25201 ctccgcgctg gcgcgcccga tgtagagccg cttgtagatg atgatgtaca ggccgaccac
    25261 gaacatgacc gccagcagaa tcatcacgac ctggacgatc cagtgcgcct gctcgaacag
    25321 gtgcgggatg tcgagcttgg cggccagcgt ggtcgagacg tgcgtcgacg aaacccctgg
    25381 cataatgagt ggttcgtcga taacacgcgc acggtggtgg gtcaaatgtg gcagtctgtg
    25441 agcgtggcca agcagctacg cgctgtggtc tttgtgctgt gtctcggcgt tcacggctgc
    25501 acgcaagtgg acggcggcgc ggtcgagctc tcatggaagt tacgagccga aagcggttcg
    25561 cagaacacgt tcctcgactg catcacgcat ttgcagccca cggggcagct cgtggaggtc
    25621 gcgaaaatcc ggctcgaatg ggacgttgac ggcgcgacag gaacgcgcag ttgggagtgc
    25681 gacgacgatc acggggtcac gaatttcgag cttccagaag gacgtgcact gcttcacgtc
    25741 agtccgatct gcgcgaacga catggttgcc gacgcgaaca cattcagatc gcctgctccc
    25801 gagcaacgca acgtgatcgt aggtgatacc gtcagcttgg gcggtatcga gctattgctc
    25861 gaagtgtcct catgtgattt gcaagcttgc atctgccagt aggcttgcgt ggcgtcacac
    25921 catcctggcg cgatccttgc cttagtcgtc cgagtgaaga gatactgagc cgcgtcgtcc
    25981 aaacgtggcc aaaccacgac acgacccgag ggagatagaa atgaagaaac ttgtcctagc
    26041 aagcttgatc gccctggccg caagccaggc tgctggttgc attatcacca gcggcgacga
    26101 tgacactggt gagaacgcct tcgtcagtgc cacctggaag ctgagaagcg agaagacgaa
    26161 caccgacgtc ccgtgcccgc cgacgttcga tacggctgcg ctgttcaacc agcccatcga
    26221 cgccaacggc aacaacgcag gcccggtcat cgtggacctg ttcaactgcg cggacaccgc
    26281 cggcaccagt gccgcgctgc cgccgacgac ctatctgacg tgggtcgaga tccagaactc
    26341 caacggcagc aacacctacg cgaagtcgtt gtcggccgtg gtcgacgtga cggtgtcgga
    26401 caagacgttc aacacccaga tcctcgtcga tggtggctac ttccagctgc agtggcagct
    26461 gcagggcgcc agcacgaacg cggcgctgac gtgtgcgcag gccaatgcgg cgggtggcgt
    26521 cgagacggtc ggcacggacg tgtcggatcc gaacaactcg aacagtgaca tcttcgactg
    26581 cgaggaccaa tacggtgtca cggccggcta cacgatgggc acgtacaccg tgtcggtcgc
    26641 ggcgctcaac tcgagcgacg cgtcagtcgg tacggcgccg gcgctcacca acaaggtgat
    26701 ccaggacaag aaccaggtga ccaacctggg cacgatcacg attccgatca ccggcatgta
    26761 agcggtctcg ccgccaagta cctcagcgcc cgcttcggcg ggcgttgttg ttttcggcgc
    26821 gtgctaaggt ttccatgtga gacgagcccc cggccgaggc agaagctgga cgatggactg
    26881 gagccagctg cggatcacgc gcggcgcgct gatgatcctc ggcatccagg tcgccctctc
    26941 tctgatctgg ctgatgggca atggcgagct ccgcgtcgac atcgcggagt gggtcgtcgc
    27001 gtcgccgagc caggtcttcg agcactacaa ggtctggacg ctcgtcacga gcccgttgct
    27061 cgagatgggg ttcctgcagc tgctcttgac cggcctcatg atgtggatgc tcgtgccgac
    27121 gctggagcgg ttctggggca cgcctcggtt cttgcggttc gtcgcgatca catcggtcgc
    27181 cggcacgatc gcgggctgcc tggtcggctg ggccatcggt agcgatgtgg cgatcactgg
    27241 tttctcgccg atgatcatcg gcgcggtcgt cgcgtttggc gtgatctacg cgcggcagcc
    27301 cgtgcagttc ttcggcgtcc tgccgctgtc gggccgcgag ttcatgttcg gcttgctcgc
    27361 agtgttcgca ctctcggtac tcctgcaggg tcactgggaa gccggtgcgg cacagggtgc
    27421 ggcggcgctt gccgcggcga tcatgacgtc gaagcggttg agcccaggtc tcgcgtggaa
    27481 gcggtggcgc atcgcgcgag cgcgggcgcg gctgtctgtc ctcgagggcg gggccaaggc
    27541 ggcgaagccg aagcccaagt ccgacgagca gcgctggctc aactaaggcc cagctcccgt
    27601 cggtgtgata cggtcggcct caatggacga gggtgacccg caattcgaat ccggcacggg
    27661 gctaaccacg ctgttcgagg gcgagtgggc gacggtccgc cgcttgcgca agggcaagtt
    27721 ggtcgtggtc agcgggcaag atcagggcaa ggaaatcgag atctcgaagc cgcgcgtcac
    27781 cggcggccgc agcatcatca gcgacctggt cgtgcaagac aaagcggtgt cgggcacgca
    27841 cttcgaagta tccgcgcgtg acgacggcta tcgcctccgc gacttgaaca gccgcaacgg
    27901 catcttcgtc ggcgatctgc gcgtgcgcga ggcgtacctg cgcccgggca cggtgttccg
    27961 catcggccac acgaacatcc agttccagtc catgcaggac gtggtcgaga tcgagctgtc
    28021 caagaaggac cgcttcgaca tgatgctcgg cgcgtcgcct gcgatgcgcg agatcttcgc
    28081 gcacctcgag aaggtcgcac cgagcgagct cacctgcctc atcaccggcg agaccggcac
    28141 cggcaaagaa atggtcgcgc gtgcgctgca caacgcgagc gcgcgcaaga acaagccgtt
    28201 cgtcgtgctc gactgcggct cgatcccacg cgagctgatc gagtcgacgc tattcggcca
    28261 cgagaagggc agcttcaccg gcgccgtcgc gcagcacacc ggctgcttcg agcaggcgaa
    28321 cggcggcacg atcttcctcg acgaaatcgg cgagctcgac atctcgctgc agccgaagct
    28381 cctgcgcgtg ctcgagcagc gcgagatcaa gcgcgtcggt ggcgatcgca cgtacaaggt
    28441 tgacgtccgc gtgctcgcgg cgacgaaccg tgacctccgc gaagaggtca accgcggcac
    28501 gttccgcgaa gatctttact tccgcctctc ggtcgtccac gtcgagctgc ccgcgatgcg
    28561 cgagcgccgc gaggacattc cgggtctcgc gaatcacttc ctgcgtgacg tcgcgtcgcg
    28621 ccgtggcatg acgatgtcgt tctcgcagga cgcgatggcc gcgatgatga gccacagctg
    28681 gcccggcaac gtgcgcgaga tgcgtaacgt cgtcgagcgc gcagccgcgc tcagcgatgg
    28741 cccggtgatc acgcgcgccg atctcgtgtt tggccgcgag atgggcccgt cggtcgtcgt
    28801 ctcgcacgac ctcgcgcagg ccggcgcgca agccgccaag cgcgcagccg ccgagctctc
    28861 gggtgtcgag ctgccgcagc aatcgggccc ggcgatcttc gacgcgtcgc tgctcaaggc
    28921 cggccttggc ttcaagcagg cgaagcagac cgtcgtcgac agcttcgaga tcgcctatct
    28981 gcaggcgctc atggttcgca acgacggcaa catcacgcgc tccgcgcagg aagcgggcct
    29041 cactcgctac cacctgcgcg agctgctcaa gcgtcacggc ctcaagggcg gcgacgtcga
    29101 gtaacccgtg ccgcgcgcgg tgctcgatcc cgcgttgagc gtgcgcgtgc tgggcatgcg
    29161 cgagctgcgc taccaggaca cgccggcgct cggcgacgat cgcccgcagc acgttcgcgc
    29221 cgcgtcggga ctcgcgatgg tcggcggccg gctcgcggtg atccaggacg actgcgcgtt
    29281 catcggtctc gtcaccgatc gcgtcagcgc gctcgcgttg ccgcgcgggc ccggcgggcg
    29341 ccgccgcttc gaggaacaga tcggcaacaa gggcgacaag ctcgatctcg aggcatgcat
    29401 cgtcgtcaaa ggtccgcttg acgaagagct gtgggcgttc ggctcgggct cgacgccgat
    29461 gcgcgagaag atcgcggtcg tcggcttttc gacgcggctc cacgacggcg cgccgctcta
    29521 tcggcgcctg cgcgaggagc tgtcaggccc gacggccgcc gaatcgtttc gcgtgccgcc
    29581 ggtcgtcaac atcgaaggcg cgtgcgtggt cggcaagcag ctgtggctgt tccatcgcgg
    29641 caacacgggt ccgcacgacg cgggcccgac ggtgtttcgc atcgatcagc ccggcttcgc
    29701 gcgctggctc gcgagcgagg gtgcattgcc cgagatcgac tatgcgcagg cgtacgacct
    29761 cggcaccgcg aatggcgtgc ggctcggctt caccgatgcc gtcgcgcacg gtgcgacggt
    29821 gttctatctc gcggtcgccg aagcgtcgcc gaacgcgatc gacgacggtc cggtgctcgc
    29881 gtcgcacatc ggcatcatcc agggtgacta cgtgcgcgcg tgcgagctcg tgatcgacgg
    29941 cgcacctgcg aaggccgaag ggctcgcatt cgatccgagc gatcgcaacc acgcctgggt
    30001 gtcgatcgat ccggatgatg tcgatcagcc ggcgcgcctc tacgagctcg agctcgtcgg
    30061 cccttggtag tcagcgaatc gcttcgagga tttcttcgcg ggtcgcgacg cccttctcga
    30121 cgagcagcga caagagcttg cgcagcacgt cctcgtcgcg cgcgacgagt gcctcgagat
    30181 tcgagatgcg atcttgcagc gcatcgatct cggcgtcgcg attcacgagc ggcggtggcc
    30241 cgacgagctc gccgatcgca ggcggcgacg gcgcggttgg aacctcgagc ggcggtggcc
    30301 gcgttttctt tggcgtgggg cgcaagccct cgcggcggcg cgcagcttcg atctcggctt
    30361 cgtcgggacg cgggcctgac ggtccgaggc cgagcgagac cgtgtcgccc gcggtgtcga
    30421 gtggaacctc gccgggtctg cggatctggc gaccgaagcc gcggcggtag tgcttcgcga
    30481 gcgcgcgctc gatcatcttc gggcccgcga gatacggccg gatgttgagc tgcgtgcgaa
    30541 tgcgcagctc gtcgatgatg ccgaggtt
//
LOCUS       DASUFX010000532        12547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_362953,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000532 DASUFX010000000
VERSION     DASUFX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362953"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFV99_21370"
     CDS             <1..524
                     /locus_tag="VFV99_21370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5061939.1"
                     /translation="SFTPLSDSLPPEQLASTMARFYDVTSTIAFEHGATIDKFIGDAV
                     MAILGAPAAMDEHEQVRRALDLATAWHTQVAKQIPGDRPLLLRIGIHQDVVAVGTFGG
                     RRRTDYTVLGIGVNLAARLEQACPPGSILVSQTVWNQLPTQPVAEPRELQLKGIPGTV
                     RAYAITPTLPAAN"
     gene            625..1398
                     /locus_tag="VFV99_21375"
     CDS             625..1398
                     /locus_tag="VFV99_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061940.1"
                     /translation="MTRMLWVALLASVGCKKKVDNPPPPAATKVEVAAKAEPPPPPVE
                     PTLDPVRVEAKKANREALGVMTKSIQCPQQAPSTFVRYCLAADDFASGTASELPDGDT
                     VIVGEMSIIQPGQPNVATLGTPMPVAFLLRKQGTTVSAKQVVFQNQDNDQMATLAARI
                     GKTIDGKWDHVDVPAVFADQIKGLSTAALDPLDQADGALWPHDTDGMELRRAKGRWLL
                     VAAPEGPEGRHVIGIFADKHKVLGPKEKSPQTVTVVHWK"
     gene            1747..4551
                     /locus_tag="VFV99_21380"
     CDS             1747..4551
                     /locus_tag="VFV99_21380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061941.1"
                     /translation="MRMITLGLAAALSAGLVHESEAVIRTKGADAPLVSAGRGPRTHR
                     TIDWKTVGSGKLLGWTAMWDRDTDVPLRLWGHTAPMFGVVASPIAAETAARAFLAEHL
                     DVLAPGAKLDDFVLVSNVLSPNGDIRSVGFVQHAGGMHVLGGAIGFAFKADRLTLVSS
                     TSLPNITVTAPAQRRSAAELTTRAQRWLAQDGYRVTISSVGTEPVIVPVVRPRIGART
                     DIEYRVAEQLEVTATNAPGAWHVWIDAGSGAPIARASTISFASGKVLFDAPDRYPSGT
                     RTGKAAAFASVTIDGTAATTALDGSVTWAGAGSATVRARGTYVNVTTQSGTAVTGTLT
                     LVSGGDATWSQATSETGDAQVNAYVHANIAKQFVRTNLNPSLAWLNQALPVSVNESGG
                     CNAFSNGNDIHFLRKNTQCENTGRLADVVYHEFGHSLHNNSIIDGVGQFDSALSEGLG
                     DILAMLITHDHGMGKGFFLSNPSSAMRELDPPNMDKKWGVDTTGEPHDDGEIIGETFW
                     DLLVGMEASLGAGAGFDKTADIYYTVMQRATDIPSSYAEALLGDDDDGDIANGTPDQC
                     AVHAAFALHGLASGAPPMGTVDKPVRTGFDISVKAQTEGGSMAACPGPMISAGEIAWR
                     VRGGTDAVIAMTLANDTYSGSIPSQPNGTVVEYKITLTLSNGTKVQYPNNPADPYYQW
                     YIGPVTPLWCGDFENGAAEWTSSTDWEAGMPAGLGNDPRAAFGGMGVYGIDLTQDGTY
                     AASAMSFAESPEIDLQGKTGLRLQYQRWLGVEDGFYDHARLKINDSEVWKNYASAAEP
                     QQNGVNHIDREWRFADFDLASFEASGKLKLRFELEADQGLQFGGWTLDDVCVVAAATG
                     PGDPNCGNGAVDANEACDDGNVTDGDGCSATCQNEDGGDGPGGGSDDGGCCNVGGSPQ
                     GALALALVTLGCILRRRRRR"
     gene            complement(4788..5621)
                     /locus_tag="VFV99_21385"
     CDS             complement(4788..5621)
                     /locus_tag="VFV99_21385"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5061942.1"
                     /translation="MLPYVELPTLGPVTSFGILAMLGALFGLTAGLRHVQRLGLDVDV
                     ARRIATYCAVGGVLGAHYVDLFFYQPGWWHHSDAVWRFINPFAGISSYGGLIGGTLGF
                     VIYTRSLGGQRLRYADAATLGVVVLMTFGRAGCASVHDHVGVATSFPLAVDFPARSGV
                     VGPHHDLGLYELVLWLVILAASVVVLRRPRRAGWYLGVVSIAYATPRFFLDMLRRAAS
                     DPRYATLTPAQWCCLFTLAIGVVLLLRSRRQPAPTPYLSVTPWRTYIREWLPRFAVTR
                     R"
     gene            5672..6187
                     /locus_tag="VFV99_21390"
     CDS             5672..6187
                     /locus_tag="VFV99_21390"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5061943.1"
                     /translation="MRTTLLLTAVGADRTGLVESLAQRIAAAGGNWEESRLARLGGQF
                     AGIVLVTIDDAKTDALIAKLRELESNGLQVTSRVVPATPVATTGMRVRLAVTGSDRPG
                     IVRDVSRILAERAVNVEELESSVGSAPMSGEAMFVARARLLVPASLELSTLRSALESL
                     GNELMVDLSAE"
     gene            complement(6188..7072)
                     /locus_tag="VFV99_21395"
     CDS             complement(6188..7072)
                     /locus_tag="VFV99_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061944.1"
                     /translation="MRAPGGDVAKRLMHEGDYAARGGVVIATSECGNYGEDTLTAWKL
                     ADGAVVVERECKDGTVLCWQIDATDTLRTSSEAWGSGGIVGLETDLVAIAQDGEEIAI
                     THFEFSSPWGGASDRDVAMLAEAIANEARCTLDIVPTKYPESMTRKPEVAAPTPAPEP
                     PPPQVGRREPLLADSAGAEVLASIVGGKETNEELRAIRLADGNVWIENEATGARWQIT
                     RDNTLWITPYVMATSSDDGVETFLEREPPVSPEQIAAFKCWADERKGPDEAAVAEFVA
                     KVVAATGCKVVHRELVWP"
     gene            complement(7053..11024)
                     /locus_tag="VFV99_21400"
     CDS             complement(7053..11024)
                     /locus_tag="VFV99_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061945.1"
                     /translation="MDHTGTIVAERFELEKLAGRGGMGAVYRAKDRQTGNFAAVKILR
                     DETTDEHRERFIREARVMSELTHPAFVRCLGFGSEGERPYLAMEWLEGEDLAQRLQRE
                     PLTTHDTLRLVARVAAALGSAHSRGIVHRDVKPSNLFLVDGDVDRVKVLDFGIAQVAR
                     NGSAAMTRTGATLGTPRYMAPEQARGGKEVDPRADVFSLGCVLFECLVGHPPFGGDNP
                     IAVLMKILLDDAPRISTILKGAPASIDDLVARMMDKEPTARPVDGLAVASQLEAIGPI
                     EGIGPTEAPERDSAVTIGEQRLVSVVLATTHVVDRPLVPPLETAPTMASEPSAPSATG
                     DNSAVMKELTRRLAATVSEYRGRSAVLPDGTIAVTIAGQGAATDQAARAARCALAIRD
                     IMPGSAMALAIGRSVTVDRAVGEVIDRAAAMLRIPTESINGTRPIRVDEVAAGLLDVR
                     FAIHGDETGLLLARERDVTDIKRTLLGKPTPCVGRERELGTLHAMFAECVSEPKAHIV
                     VVTAPAGVGKSRLRHELAQRIINAGGPQPELWIARGDPLSAGAPFAMLAQLLRRLAGI
                     IDDEPLSSRQRKLKARVLRNVTRDGTRITEFLGELAGAPSRDEPSLQLRAARQDPQLM
                     GDQMLRAWEDFVEAETGAGPIVIMLEDLHWGDMPTVRAMDTIIRQLPERPLFVLALAR
                     PEVDSVFPRLWHERNVERISLGELTRRAAEKLIREALGNVDAAMLTRLIDRAAGNAFY
                     LEELIRSVAEGKGDRLPETVVAMAQARIEGLELDARQVLRAASIFGFAFWPAGVAALL
                     EGNVEVSGWIEQLVERELVTPMRESRLGEEREYRFRHALVREAAYAMLPERDRKRGHF
                     RAGEWLESAFRADDHGAAIEAVVLAEHFERGGEPRRASGWYQRAAEQALEADDLAAAI
                     DRAERAITALQSAGTEISTEDRERIGVLRQLQSDAHIWRGEYPASAERGCEALDALPP
                     ASVQWLNAAATVADAWCRLLVHGDVVELCKTLVDLPLTPELHSAYTHAVAMTMPTVLW
                     HTDGNIDAALFQRLDSVEAALDQNDPIALGWSCCARSWRALRDGDLGECHRLDSMIVE
                     CFTAVADQRHVCQQRAYVGYDELMIGAYPQAEQSLREAIAMGERMGLHQVTAQAQHNL
                     GLVLCRLGRLDEARAVETAAMRALDEQGNRRLAITARNYLGIIEIEAGNFAEAIRYVT
                     DAIDNGGDMQSALTSFLATASTACRLSGDNAGALSHAKRALELIALHGRPEEGEAAIR
                     LAFAHALRASGQHEESLAAIRSAEEELLAAAAKIKDERWRSVFLESVPENAETIALAR
                     AWR"
     gene            complement(11044..12417)
                     /locus_tag="VFV99_21405"
     CDS             complement(11044..12417)
                     /locus_tag="VFV99_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061946.1"
                     /translation="MIVPAFAHANTPEDVYGSSAKTIAMGGAGTALPGDFSSAHYNPA
                     GLSFCHDDLLALDVSYIAHHLTFTDEDTARTEPLVPKETRNQTRFMLGSCLDLPFDLS
                     AGIMLGFGTPGAIRLDQQTANETPHYVMYGEANEQLSMALGFSWRASKQLSIGAGFAA
                     LFKSRLPLDADFPILEPDPTDPSGYRPVSFGLGIGLGAKLAPRFGVLWTPSPRFRIGA
                     AYRGPLFHDLDVDAYIKAKLIIEIPVPVHVDSLGWFSPRQFSAGASGEPTENLTLTAD
                     VTYYRWGELRTSESSYPFLNMYSLDPMGASGALVFPRPIRPGWKNNLAWRAGGEWRSP
                     GGGMAVRGGFGYRTRGVNSAENSNVNLLDAPTITASTGVAWFAGTRAAAPAAPRRKKR
                     TSEPPRWWRYTLPDVNFAADAFIRVDHMLEQQVDHTSTPGAAIPEKHYRFGGQVFQFG
                     LMATLGW"
BASE COUNT         2194 a         4159 c         4190 g         2004 t
ORIGIN      
        1 tgtcgttcac gccgctcagc gactcgctgc cgcccgagca gctcgcctcg acgatggcgc
       61 ggttctacga cgtgacgtcg acgatcgcct tcgagcacgg cgcaacgatc gacaagttca
      121 tcggcgatgc cgtgatggcg atcctcggcg cacccgcagc catggacgag cacgagcaag
      181 tgcggcgggc gctcgatctc gcgacggcct ggcacacgca agtcgcgaaa caaattcctg
      241 gcgaccgtcc gctgttgctg cgtatcggca tccatcagga cgtcgttgcg gtcggcacgt
      301 tcggtggtcg tcgccgcacc gactacactg tgctcggcat cggcgtgaac ctcgcagcgc
      361 gcctcgagca agcgtgcccg cccggcagca tcctcgtctc gcagaccgtg tggaaccagc
      421 tgcccacaca gccggtcgcc gagccgcgcg agctccagct caagggcatc cccggcaccg
      481 tgcgcgcgta cgcgatcacg ccgacgctgc cagcggcgaa ctgatcatca gggtgcacct
      541 ggtgcactct ccgagcggcg aatggaatct cgcattgtta cctgccgacg cggctggcac
      601 tgtgcgtgca caatgtggcc cgccatgaca cgcatgctct gggttgctct gctcgcgtcg
      661 gtcggttgca agaagaaggt cgacaatccg ccgccgcccg ctgcgacgaa agtcgaggtc
      721 gcggcgaagg cagaaccgcc gccgccgccc gtcgagccga cgctcgatcc ggttcgagtc
      781 gaggcgaaaa aggccaatcg cgaagcgctc ggcgtgatga ccaagtcgat ccagtgcccg
      841 cagcaggcgc catcgacgtt cgtgcgctac tgcctcgccg cggacgactt cgcatcgggc
      901 actgcgtcgg agcttcccga tggcgacacc gtgattgtcg gcgagatgag catcatccag
      961 ccgggacagc cgaacgtcgc gacactcggc acgccgatgc cggtggcgtt cctgctccgc
     1021 aagcaaggca cgacggtgtc ggcgaagcag gtcgtgttcc agaaccagga caacgatcag
     1081 atggcgacgc tcgccgcgcg aatcggcaag acgatcgacg gcaagtggga tcacgtcgac
     1141 gtgcctgcag tgttcgccga tcagatcaaa gggctctcca ccgcagcact cgatccgctc
     1201 gaccaagcag acggcgcgct gtggccacac gataccgacg gcatggagct gcgccgcgcg
     1261 aagggtcgct ggttgctcgt cgccgcaccc gagggtcccg agggccgcca cgtgatcggc
     1321 atcttcgcag acaagcacaa ggtgctcggg cccaaagaga agtcgccgca aacggtcacc
     1381 gtcgttcact ggaagtgaga ctgcacttcg ctacgaggtg cgcccgcgga atcgaaacgc
     1441 gcgacgaaaa tcgtaagcag aagcacgcca cattgcgcgc tcgaacccgc gcgacggtag
     1501 tgacgctgcc tctagtgcgc gttttccgtc gaggaacgca ttcgcaccgc gccggcgcgc
     1561 gcactcgaga tgcacttgat gtgcacgcgc tcggatttct aacgcggact atcccaaatt
     1621 cttgttgact atccgtcaac aagcgtgctc gacgcatcac tagcatcaag ggatgtgagg
     1681 agcgccgact tggcgccggt gtcggacgcg tgtgaactga gaacacacga agaggaggct
     1741 ttgccgatgc gtatgatcac gctcgggctc gccgctgcat tgtctgcggg gctcgttcac
     1801 gagagtgaag ctgtcattcg aacgaagggt gccgacgcgc cattggtctc cgccggacga
     1861 ggaccgcgta cgcaccgcac gatcgactgg aaaaccgtcg gcagcggcaa gctcctcggt
     1921 tggacagcga tgtgggatcg cgacaccgac gtgccgctgc gactatgggg acacacggcg
     1981 ccgatgtttg gcgtcgtcgc gagtcccatc gcggccgaga ctgcggcgcg cgcattcctc
     2041 gccgagcatc tcgacgtgct cgcgccgggg gcaaagctcg acgacttcgt gctcgtgtcg
     2101 aacgtgctgt cgccaaacgg cgacatccgc agcgtcggct tcgtgcaaca cgccggcggc
     2161 atgcacgtcc tcggcggcgc gatcggtttt gccttcaagg cggaccggct cacgctcgtg
     2221 agctcgacct cgctgccgaa catcaccgtg accgcacctg cgcaacgccg ttcggctgcc
     2281 gagctcacca cgcgcgcgca gcgctggctc gcgcaagatg gatatcgggt gacgatctcg
     2341 agcgtcggta cagagcccgt catcgtgccg gtcgtgcgtc cgcgtatcgg tgcgcgaacc
     2401 gacatcgagt atcgcgtcgc cgaacagctc gaagtgaccg cgacgaacgc gccaggcgcg
     2461 tggcacgtgt ggatcgatgc cggcagcggc gcgccgatcg cacgcgcgag cacgatctcg
     2521 tttgctagcg gcaaagtgct gttcgacgcg cccgatcgct atccgtcggg aacgcgcacg
     2581 ggcaaggccg cggcgttcgc cagcgtcacg atcgacggca ccgccgcgac gaccgcgctc
     2641 gacggctcgg tgacgtgggc cggcgccggc tcggcaacgg tacgcgcacg cggcacgtac
     2701 gtgaacgtga cgacccaatc cggcaccgcc gtgaccggca cgctaacgct tgtgtcgggc
     2761 ggcgatgcga cgtggtcgca ggcgacgagc gagaccggcg acgcgcaagt caacgcgtac
     2821 gtgcacgcga acatcgcgaa gcagttcgtg cggacgaatc tcaacccgtc gctcgcgtgg
     2881 ctgaatcaag ccctacccgt tagcgtcaac gagagcggcg gctgcaacgc gttctcgaac
     2941 ggcaacgaca tccacttcct gcgcaagaac actcagtgcg agaacaccgg gcgacttgcg
     3001 gacgtcgtct atcacgagtt cggtcactcg ctgcacaaca actcgatcat cgacggcgtc
     3061 ggccagttcg atagcgcgct ctccgaaggt cttggcgaca tcctcgcgat gctcatcacg
     3121 cacgaccacg gcatgggcaa gggcttcttc ctgtcgaatc cgtcgtcggc gatgcgcgag
     3181 ctcgatccgc cgaacatgga caagaagtgg ggcgtcgaca cgaccggtga gccacacgat
     3241 gacggcgaga tcatcggcga gacgttctgg gatctgctcg tcggtatgga ggcatcgctc
     3301 ggtgcgggtg ccggcttcga caagacagcc gacatctatt acacggtgat gcagcgcgcg
     3361 acggatatcc cgtcgagcta cgccgaagct ctgctcggcg acgacgacga cggcgacatc
     3421 gcgaacggca caccggatca gtgcgcggtg cacgcggcgt tcgcgctcca cggtctcgcg
     3481 agcggcgcac cgccgatggg taccgtcgac aagccggtcc gcaccggctt cgacatctcg
     3541 gtgaaggcgc agaccgaagg cggctcgatg gccgcgtgcc cgggaccgat gattagcgcc
     3601 ggcgagatcg catggcgtgt gcgcggcggc accgatgccg tcatcgcgat gacgctcgcg
     3661 aacgacacgt actccggctc gattccgtcg cagccgaacg gcaccgtcgt cgagtacaag
     3721 atcacgctga cgctgtcgaa cggcacgaag gtgcagtatc cgaacaaccc ggccgatcct
     3781 tattaccagt ggtacatcgg tccggttacg ccgctatggt gcggcgattt cgagaacggt
     3841 gccgcggagt ggacgagctc gacggactgg gaagccggca tgcctgcggg cctcggcaac
     3901 gatccgcgcg ctgcgtttgg cggcatgggc gtgtacggca tcgacctgac gcaagacggc
     3961 acctacgctg cgagcgcgat gtcgtttgcc gagtcgccgg agatcgacct gcaaggcaag
     4021 accgggctgc gtctgcagta ccagcgctgg ctcggcgtcg aggacggctt ctacgatcac
     4081 gcacgtctga agatcaacga cagcgaggtg tggaagaact acgcaagcgc ggccgagccg
     4141 cagcagaacg gtgtcaacca catcgatcgc gagtggcggt tcgccgactt cgatctcgca
     4201 tcgttcgaag caagcggcaa gctcaagctg cgtttcgagc tcgaggccga tcagggcctg
     4261 cagttcggcg gctggacgct cgacgatgtc tgtgttgtcg ccgcggcgac gggtcctggc
     4321 gatccgaact gcggcaacgg cgcggtcgac gcgaacgaag cctgcgacga cggcaacgtc
     4381 accgatggcg acggttgctc tgcgacctgc cagaacgaag atggcggcga tggaccgggc
     4441 ggcggcagcg atgacggtgg ctgctgcaat gtcggcggca gcccgcaagg tgcgctcgcg
     4501 ctcgcactcg tgacgctcgg ctgcatccta cgtcgacgtc ggcgccgcta ggcggcatcg
     4561 ccagcccagc cgttcgacgc gcgcgacctc ttagtgctgt tgcgcgcgtg cctcgacgcg
     4621 gcccttgatc gcgagctgca cgagcacgat gaccgttcct acggtgtagt cggcgacgat
     4681 gctgggcagt ccaagcagtg agcgaatcat tggtccagcg tagcgaacgc gtgtttctct
     4741 tccgtttcgc cactggaaaa cgttcgcgac acgtcgggag tggcgagcta tcgccgagtt
     4801 accgcgaaac gcggcaacca ttcgcgtatg tacgtgcgcc atggggttac agacaagtac
     4861 ggcgttggcg ccggttgcct gcgacttcgc aacaacagaa caacaccgat cgcgagcgtg
     4921 aatagacaac accattgcgc cggtgtgagc gtcgcgtagc gcggatcgct cgcggcgcgg
     4981 cgaagcatgt cgaggaaaaa gcgtggcgtc gcatacgcaa tagaaaccac gccgagatac
     5041 caacctgccc tccgcggacg ccgcagcacg acaaccgacg ctgcaaggat cacgagccac
     5101 aacacgagct cgtagagacc gagatcgtga tgcggcccga caacgccgct gcgtgcagga
     5161 aaatcgaccg cgagcggaaa gctcgtcgcg accccgacgt gatcgtgaac cgacgcgcaa
     5221 cccgctcgcc cgaacgtcat gagcacgacg acgccgagtg tcgctgcatc ggcgtaacgc
     5281 aagcgctgcc cgccgagcga gcgcgtgtaa atcacaaagc cgagcgtgcc gccgatgagg
     5341 ccgccatacg acgagatgcc ggcgaacggg ttgatgaacc gccacaccgc gtccgagtga
     5401 tgccaccacc ccggttgata gaagaagaga tcgacgtagt gcgcaccgag cactccgccg
     5461 accgcgcagt aggtcgcgat gcgccgcgca acgtcgacat cgaggccgag tcgctgaacg
     5521 tggcgcagtc cggcggtgag gccgaacaag gcacccaaca tcgcgaggat gccgaacgac
     5581 gtgacggggc caagggtcgg tagctcgacg tacggcagca tgtcgggcaa aaccggagcg
     5641 cacgcccccg cattcccggt aggcttagcg tatgcgcact acgttgctcc tcactgctgt
     5701 cggggcggat cgcaccggcc ttgtcgaatc actcgcccag cggatcgccg ccgcgggtgg
     5761 caactgggag gagagccgac tcgcgcgtct cggtggtcag ttcgcgggca tcgtgctggt
     5821 cacgatcgac gatgcgaaga ccgacgcgct gatcgcgaag ctgcgcgagc tcgagagcaa
     5881 tggcctgcaa gtcacgtcgc gcgtggtgcc ggcgacaccg gtggcgacga caggcatgcg
     5941 cgtgcgcctc gcggtgacgg gcagcgatcg gcccggcatc gtgcgcgatg tgtcgcgcat
     6001 cctcgccgag cgcgcggtca acgtcgagga gctcgagtcc tccgtcggca gcgcgccgat
     6061 gtcgggcgag gcgatgttcg tcgcgcgcgc acggttgctc gtgccggcat cgctcgagct
     6121 ctccacgcta cgcagcgcgc tcgagtcgct cggcaacgag ctgatggtcg acctttcggc
     6181 cgagtagtca cggccagacg agctcgcggt gcacgacctt gcagcccgtc gcggcgacca
     6241 ccttcgcgac gaactctgcg accgcggcct catcgggacc cttccgctcg tcggcccagc
     6301 acttgaaggc cgcgatctgc tccggggata cgggcggctc gcgttccaag aatgtctcca
     6361 caccatcatc gctcgaggtc gccataacgt aaggcgtgat ccacagcgtg ttatcgcgcg
     6421 tgatctgcca tcgcgcgccg gtcgcttcgt tctcgatcca tacgttgccg tcggcgagcc
     6481 tgatcgcacg cagctcctca tttgtctcct tgccgccaac gatgctcgcg agtacttcgg
     6541 cgccggccga gtctgcaagc aacggttcgc gccgtccgac ctgcggcggc ggtggctccg
     6601 gcgcaggcgt tggcgcggcg acctcgggct tccgggtcat ggactcggga tacttggtcg
     6661 gcacgatgtc gagcgtgcag cgcgcctcgt tcgcaatcgc ttcagcgagc atggctacgt
     6721 cgcggtcact cgccccgccc cacgggctgc tgaattcgaa gtgcgtgatc gcgatctctt
     6781 cgccatcctg cgcgatcgcg acgaggtcgg tttccagacc gacgatgcca ccggatcccc
     6841 atgcctccga cgacgtgcgc agcgtatcgg tcgcgtcgat ctgccagcac agcacggtgc
     6901 cgtctttgca ttcacgctcg acgacgacgg cgccatccgc gagcttccac gcggtgagcg
     6961 tgtcttcgcc gtaattgccg cactcgctcg tcgcgatcac cacgccgcct cgcgccgcgt
     7021 agtcaccctc gtgcatcaat cgcttcgcca cgtcaccgcc aggcgcgcgc aagtgcaatc
     7081 gtctcggcgt tctcgggaac cgactcgagg aagaccgacc gccagcgttc gtccttgatc
     7141 ttcgcggcag cggcgagcag ctcttcctcg gccgagcgga tcgcggcaag cgattcttcg
     7201 tgctgaccgg atgcacgcag cgcgtgtgca aacgcgagac ggatcgcggc ttcgccctcc
     7261 tcgggacgtc cgtggagtgc aatcagctcg agcgcgcgct tggcatgaga gagcgcaccc
     7321 gcgttgtcac cggagagccg gcacgcggtc gacgcggtcg cgaggaacga ggtgagcgcg
     7381 gactgcatgt caccgccatt gtcgatcgcg tcggtgacgt accggatcgc ttcggcgaag
     7441 ttgccggcct cgatctcgat gatgccgagg tagttgcgcg ccgtgatcgc gagccggcga
     7501 ttaccctgct cgtcgagcgc gcgcatcgca gcggtctcga cagcgcgggc ctcgtcgagg
     7561 cggccgaggc ggcagagcac gaggccgagg ttgtgctggg cctgcgcggt gacctggtgc
     7621 aggcccatgc gctcgcccat cgcgatcgcc tcgcgcaatg actgctcggc ttgtgggtac
     7681 gcaccgatca tcagctcgtc gtagccgacg tacgcgcgct gctggcaaac atggcgttga
     7741 tcggcgacgg ccgtgaagca ctcgacgatc atcgagtcga gtcggtgaca ctcaccgagg
     7801 tcgccgtcgc ggagcgcacg ccacgagcga gcgcagcagc tccagccgag cgcgatcggg
     7861 tcgttttgat cgagagcagc ttcgaccgag tcgagacgct gaaacagcgc ggcatcgatg
     7921 ttgccgtcgg tgtgccacag cactgtcggc atcgtcatcg cgactgcgtg ggtgtacgcg
     7981 ctgtgcagct cgggcgtgag cggcagatcg acgagcgtct tgcacagctc gacgacatcg
     8041 ccgtgtacga ggaggcggca ccacgcatcg gcgacggtcg ccgctgcatt cagccactgc
     8101 acactcgccg gcggcagggc atcgagcgct tcgcatccgc gttcggccga cgcgggatac
     8161 tcgccgcgcc agatgtgagc gtcggactgg agctgacgca ggacgccgat ccgctcgcgg
     8221 tcctcggtgg aaatctcggt gccggccgat tgcagcgcgg tgatcgcgcg ctcggcgcga
     8281 tcgatcgcgg cggcgaggtc gtcggcttcg agcgcctgtt cggcggcacg ctgataccaa
     8341 ccgctcgcgc gtcggggctc gccaccgcgc tcgaagtgct cggcaagcac gactgcctcg
     8401 atcgcggcac cgtggtcgtc ggcgcggaac gccgactcga gccattcgcc ggcgcggaag
     8461 tggccgcgct tgcgatcgcg ctcgggcagc atggcgtagg cggcttcgcg aacgagggcg
     8521 tggcggaagc ggtattcgcg ctcttcgccc aggcgcgact cgcgcatcgg ggtgacgagc
     8581 tcgcgctcga cgagctgttc gatccagcca ctgacttcga cgttgccttc gaggagcgca
     8641 gcaacgccgg ccggccagaa cgcaaaaccg aagatgctcg cggcgcgcag gacctggcgc
     8701 gcatcgagct cgagaccttc gatacgcgct tgcgccatcg cgacgacggt ctccggcaac
     8761 cgatcgccct tgccttccgc aaccgaccga atcagctctt cgaggtagaa cgcgttgcct
     8821 gccgcgcgat cgatcagacg ggtgagcatg gccgcatcga cattgccgag cgcttcgcgg
     8881 atcagtttct cggcggcgcg acgcgtgagc tcgccgagcg agatgcgctc gacgttgcgc
     8941 tcgtgccaca ggcgcgggaa cacggagtcg acctcgggac gcgcgagcgc gagcacgaac
     9001 aacggtcgct cggggagctg gcggatgatc gtgtccatcg cgcgcaccgt cggcatgtcg
     9061 ccccagtgca gatcctcgag catgatcacg atcgggcccg cgccggtttc ggcctcgacg
     9121 aagtcctccc aggcacgcag catctggtcg cccatgagct gcggatcttg gcgggccgcg
     9181 cgcagctgca agctcggctc gtcacggctc ggtgcaccgg cgagctcgcc gaggaactcg
     9241 gtgatgcgcg tgccgtcgcg ggtgacgtta cgcaacacgc gcgccttgag cttgcgttga
     9301 cggctcgaca gcggttcgtc gtcgatgata ccggccaacc gccggagcag ctgcgcgagc
     9361 atcgcgaacg gggcgccggc actcagcgga tcgccgcgcg cgatccagag ctcgggttgc
     9421 gggccgcccg cgttgatgat gcgttgcgcg agctcgtgac ggagccgcga cttgccgaca
     9481 cccgccggcg cggtgacgac gacgatgtgc gccttcggct cggagacgca ctcggcaaac
     9541 atcgcgtgca gcgtgccgag ctcgcgctcg cgaccgacac atggcgtcgg cttaccgagc
     9601 agcgtgcgtt tgatatcggt gacgtcacgc tcgcgggcga gcaacaggcc cgtctcgtcg
     9661 ccgtggatgg cgaagcgaac gtcgaggagg ccggcagcga cctcgtcgac gcgaatcggc
     9721 cgggtgccat tgatcgattc ggtcgggatg cgcagcatcg cggctgcgcg atcgatcacc
     9781 tcgccgaccg cgcgatcgac agtgacactg cgaccgatcg cgagtgccat cgcggagccc
     9841 ggcatgatgt cgcggatcgc gagcgcacaa cgcgccgcac gcgcggcttg atcggtagcg
     9901 gcgccctgcc ccgcgatcgt gaccgcgatc gtgccatcgg ggagcacggc cgagcgcccg
     9961 cgatactcgg agacggtcgc agcgagccgt ctcgtcagct ccttcatcac cgccgagttg
    10021 tcgcccgttg ccgacggcgc agatggctca cttgccatcg tcggtgcggt ctcgagtggc
    10081 ggcacgaggg ggcggtcgac gacgtgcgtg gtcgcgagca cgaccgagac caagcgctgc
    10141 tctccaatgg tgacggccga gtcgcgctcc ggcgcttccg tcgggccgat gccctcgatc
    10201 ggtccgatcg cctcgagttg cgacgcgacc gcgagtccgt ccacgggccg cgctgtgggc
    10261 tccttgtcca tcatgcgcgc gacgaggtcg tcgatcgatg cgggcgcgcc cttgaggatc
    10321 gtgctgatcc ggggcgcatc gtcgagcaag atcttcatca gcaccgcgat cgggttgtcg
    10381 ccgccgaatg gcgggtggcc gaccaggcac tcgaacaaca cgcagccgag cgagaacacg
    10441 tcggcgcgtg gatcgacttc tttcccaccg cgcgcttgct cgggagccat gtagcgtggc
    10501 gtgccgagtg tcgcgcccgt gcgcgtcatc gcagcggaac cgttgcgcgc gacttgcgcg
    10561 atgccgaagt cgagcacctt cacgcgatcg acgtcgccat cgacgaggaa caggttgctc
    10621 ggcttgacgt cgcgatgaac gatgccgcgc gaatgcgccg agccgagcgc ggcagcgaca
    10681 cgcgcgacga ggcggagcgt gtcgtgcgtg gtgagcggct cgcgctgtag acgttgcgcg
    10741 aggtcttcgc cttcgagcca ctccatcgcg agatacggcc gttcgccttc gctcccgaag
    10801 ccgagacagc gcacgaacgc cgggtgagtg agctccgaca tcacacgcgc ttcgcggatg
    10861 aaacgctcgc ggtgctcgtc agtcgtctcg tcgcgcagga tctttactgc cgcgaagttg
    10921 ccggtctgcc gatccttcgc gcggtacact gcacccatac cgccgcggcc cgcgagcttc
    10981 tccagctcga agcgttccgc cacgatggtg ccggtgtggt ccaccgatcc catggtaacc
    11041 gactcaccag ccgagcgtcg ccatcaggcc gaactggaac acctggccgc cgaagcggta
    11101 gtgtttctcg ggaatcgccg cgccgggtgt gctcgtgtgg tcgacctgct gctcgagcat
    11161 gtggtcgacg cggatgaacg catcggcggc aaaattgacg tcggggagcg tgtaccgcca
    11221 ccagcgcggc ggctccgatg tgcgcttttt gcgtctcggc gcggcgggcg cagccgcacg
    11281 cgtccccgca aaccaggcga ccccggtgct cgcggtaatc gttggtgcat caagtaagtt
    11341 cacgttcgaa ttctcggcgc tgttgacgcc gcgcgtgcga tagccaaacc cgccgcgcac
    11401 cgccatccca ccgcccgggc tgcgccactc gccgccggcg cgccacgcga ggttgttctt
    11461 ccaacccggg cgaatcggcc gcgggaatac gagtgccccc gaggcgccca tcggatcgag
    11521 cgagtacatg ttgaggaacg gatagctcga ctcgctcgtg cgcagctcgc cccaccggta
    11581 gtacgtgacg tcggcagtga gcgtcaggtt ttcggttggc tcgccactcg caccggccga
    11641 gaactgccgc ggcgagaacc agccgagcga atcgacgtgc accgggaccg ggatttcaat
    11701 gatcagcttc gccttgatgt acgcgtcgac gtcgaggtcg tgaaacagcg gcccgcgata
    11761 cgcggcgccg atccggaacc gcggcgacgg cgtccacagc acaccaaacc gcggcgcgag
    11821 cttggcgccg aggccaatgc cgagaccgaa gctgaccggt cgatagcccg acggatccgt
    11881 cggatcgggc tcgaggatcg gaaagtccgc atcgagcggc agccgcgact tgaacagcgc
    11941 tgcgaagccg gcgccgatcg agagctgctt cgatgcccgc cacgagaacc cgagcgccat
    12001 cgacagctgc tcgttcgcct cgccgtacat cacgtaatgc ggcgtctcgt tcgccgtctg
    12061 ttgatcgagg cgaatcgcgc ctggcgtacc gaacccgagc atgatgcccg cgctcagatc
    12121 gaacggcaga tcgagacacg agccgagcat gaagcgcgtc tggttgcgcg tctccttcgg
    12181 cacgagcggt tcggtgcgtg cagtgtcctc gtcggtgaac gtcaggtgat gcgcgatgta
    12241 gctgacgtcg agcgcgagca aatcgtcgtg gcagaacgag agcccggcgg ggttgtagtg
    12301 cgccgacgag aagtcccccg gcagcgcggt gccggcgccg cccatcgcga tcgtcttcgc
    12361 cgagctgccg tagacatcct cgggcgtgtt cgcgtgcgcg aacgcgggaa cgatcacagg
    12421 agcgaccgcg agcaaacaaa gactgcgacg taacaaggcg agcacgatct atctaatgag
    12481 gtctaagcgt accgacgagc ttccagatcg aagtgatgcc gttgacgtcg tcgagcaaga
    12541 actcgct
//
LOCUS       DASUFX010000533         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_503244,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000533 DASUFX010000000
VERSION     DASUFX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503244"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..2671
                     /locus_tag="VFV99_21410"
     CDS             350..2671
                     /locus_tag="VFV99_21410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5061947.1"
                     /translation="MTATATPMNGSVKGRVLAKNGEPIPGAVITLGTASAISDGDGNY
                     VLEDVAAGAQTLSIEATGYATRETPIEVVAGRILGVTARMDTTQLPGEEIVVVGTRLP
                     EKRLDAPVTIEIVTDKDLKTAAGASYLSALSRVKGIDFSDAGIGDQRISARGFATQFN
                     SRMVTMVDGRLATLPGNGLPQGNLLPQSGLDMKAVEVVVGPASALYGPNAHTGVINIV
                     TKTPWDDSGAAVSVRGGTQAMFAGAARVAGTVKDDFGYKVNGEYLRAHDFVPDRATHS
                     YAPNVYEGDLLENYNVEQAKTDGTLYYKQGDWLATAGAGISDSTGFSITNAGRNHLRN
                     WQVQYQSVTLSSPHIYGQVTRTHSDAGKTYQLDRLARTVAAMGGVPANPADLNALRDS
                     ISLVDDSQLIDSELQYRHTLAGVKTTLGAQYRAYAPSSAGTYLDDKTQDISVAEAGTY
                     LQLDTMLFHRLRLAGAARVDHHTLYATQISPKAAVQYEIAENQNVRVGYNRAFKSPTV
                     LENFLRINDVLLGNRTGFVVRDAGGNVISDIAPLSPEQVDAIEVGYKAAIGSKLYVDA
                     VAYQSWYRNFISPLTSVANPMAAMPTFATYADGTPTAQGTPAEGTLSTYMNFGKAKVR
                     GIDVGVDYKPIEQLTLSTSASAMQLASFENTNPLQTDLLLNAPAFKLRGSVQGDDLGL
                     KNSFWRVDSRWHTAYEFASGYWNSMTLLGGKVPARMVLDLSLGYKLPKQGITISGTVA
                     NLLDNNDPDVLGAPIPRRFAWLQVAYDWDGLRY"
     gene            2723..3124
                     /locus_tag="VFV99_21415"
     CDS             2723..3124
                     /locus_tag="VFV99_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061948.1"
                     /translation="MRLALLTLLLIACNKDPEQHIDASVHLIDAKSVDAPPDAPPDAP
                     PDAAPPMNVVTACMHACDAIAACVQMPADQECYTDCATDLADCTAQQVATIDACSTEM
                     CGDLENNMSPLLDCIVAVSCVDMATIRGSRK"
     gene            complement(3105..4163)
                     /locus_tag="VFV99_21420"
     CDS             complement(3105..4163)
                     /locus_tag="VFV99_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061949.1"
                     /translation="MRLLALVLVVVACSSASPRAATRDDAPAAGFDANEDRRHWYAGD
                     MHMHVSPPDSHDDVMLSVTEIAAAAARAGMDFVVLTPHLWPWRWQTDHTKWERAWREL
                     AGSCAEAKTPTLIPGVEWTTGDGHFTVAGANLADLKGNDFLAAADAAGAWISVNHPFA
                     TPTKIPGVRASHYDMSYRVWTQHRAGFSAIDGAEVWNVPLGIANVISRPGGKSGEELA
                     WAELNRVVHDEHRRVTAVGGTDNHQTNVMATTWVLAVDATASAILAALRDGATCVGGP
                     EAGTLHARGDGEWVAIGGDVVGPLTTLEWKGTARVFVDDVDQGEHTDHFMHATGGDLH
                     TYRIELGNSRSGFIYANL"
     gene            complement(4197..5039)
                     /locus_tag="VFV99_21425"
     CDS             complement(4197..5039)
                     /locus_tag="VFV99_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061950.1"
                     /translation="MQNLRRVGPEALDTYLRSYGASASPMLDRVCAQKDAHTSLLYWH
                     TDQGAALVDARRTKRPILSLRLLGRLDEELSCANSRFFRKLLYPDTQINRVLREQFVL
                     HWESVRPVPIVTIDFGGGRRVERTLTGNSAHLVLDWRARPVDVLPGLFDRETFLVLLA
                     QAQVYAKADRGDLAHLHRDALRPVLPPVAPSRAFAASRIAPTKHTMEAPLLRAVQVEA
                     DADTRENLTLHDRVHEAFAAGAEWAGIPGLVEWIYAELFAMPLGDPALGLDIPDPFPI
                     RATA"
     gene            5152..6306
                     /locus_tag="VFV99_21430"
     CDS             5152..6306
                     /locus_tag="VFV99_21430"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5061951.1"
                     /translation="MYKSLIVLIALSGSARAQTPPAAPETAKTPTDTAAAVEKPAEAP
                     PPKKLSVGTKGLFNPGLLAQAWLQHDRAGDTTQISQFRLRRAELSVSGEIIPKTVGYK
                     VMFDAAKVRETQKVTVAGPPDAMGNPTTVTINNPTSAVSALQDFYITALSPFVDVSIG
                     QFKIPVSWEGYNSSGKLMFPERAVVSSTFGDKRDLGVRLEKKLATVGYSAGVFNGSGL
                     DNFDTNVQKDVALRLEAYPITGLTVAGVTYDSIGQRDHAGTKDRWEADVRYEAGDVLL
                     QSEAIFARDVTKDAMPATLGRGFYVLGGYTLHDIDSQFHGDLQPVVRVGAFDPDTDLA
                     DNALLHFDVGVNYYLIKHEMKVQAAYQRTQFQDSMKPANNELILAAQVMY"
     gene            complement(6322..6585)
                     /locus_tag="VFV99_21435"
     CDS             complement(6322..6585)
                     /locus_tag="VFV99_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061952.1"
                     /translation="MTRPRLAIGCLFALCIWAACPHSDDHPDACPAPNPPGQEKPPND
                     AGLCECNYTPVDYCACGGDAGVTDCGATCTCGICMRTCVPIER"
     gene            complement(6590..6946)
                     /locus_tag="VFV99_21440"
     CDS             complement(6590..6946)
                     /locus_tag="VFV99_21440"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5061953.1"
                     /translation="MTVAATNNSVLSLGGIASYSNFGEPSEIAAPGNWPSIGGPGMLN
                     GRTDAADISAAGTSFAAPAVAGVAALIASRNLTLYGVPDGAALKIDLLDNHTVIDPDG
                     LALIPSHKGLTMTNLR"
BASE COUNT         1283 a         2355 c         2358 g         1165 t
ORIGIN      
        1 gcaccagagt ttgcgcatga cggagctagc taccgcagga cgaaacctga gtgcagctcg
       61 atcgacagct gttggcagat gccagcaggt accacgatgc gaacgttctt cgcgccaaca
      121 gcaaacactc gacatcgtta cgacgactcg tggccttcgc gttgcactcg ggcgcgcgca
      181 gatagcggag gcaatcatga ggtcgaggag cttgctagga ctaggtgtcg tcggcgcgtt
      241 gtcggtcgtc gcgtcggcac aatctgaacc gccggcggac gcgccagcac aaccggcgca
      301 accggcagat ccggcgcctg caccggccgc ggcaccacag ggtccatcgg tgaccgcgac
      361 cgcgacaccg atgaacggca gcgtgaaggg ccgcgtgctc gcgaagaacg gcgagccgat
      421 cccaggcgcg gtgatcacgc tcggcaccgc ctcggcgatc tcggatggcg atggcaacta
      481 cgtgctcgag gatgtcgccg ctggtgcgca gacgctctcg atcgaagcaa ccggctacgc
      541 gacgcgcgag acgccgatcg aagtcgttgc cggccgcatc ctgggcgtca ccgcgcgcat
      601 ggacacgacc cagcttcccg gtgaagagat tgtcgtcgtc ggtacgcgcc tgcccgagaa
      661 gcggctcgac gcaccggtga cgatcgagat cgtgaccgac aaggatctga agacagcggc
      721 gggcgcgtcg tacctgtcgg cgctctcacg cgtgaagggc atcgatttct ccgacgccgg
      781 catcggcgat caacgcatct cggcgcgcgg cttcgcgacg cagttcaact cgcggatggt
      841 gacgatggtg gatggtcggc tcgcgacgct gccaggcaac gggttgccgc agggcaatct
      901 gctgccgcag agcggcctcg acatgaaagc ggtcgaggtg gtggtcggtc cggcgtcggc
      961 tctctacgga cccaacgcgc acactggcgt gatcaacatc gtgacgaaga cgccgtggga
     1021 tgactccggc gctgcggtat cggtgcgcgg cggcacgcaa gcgatgttcg ccggcgctgc
     1081 gcgcgttgcg ggcaccgtga aggacgactt cggttacaag gtcaacggcg agtacttgcg
     1141 cgcgcacgac ttcgttcccg atcgcgcgac gcactcgtac gcgccgaacg tctacgaagg
     1201 cgatctgctc gagaactaca acgtcgagca ggcgaagacc gacggtacgc tctactacaa
     1261 gcaaggtgac tggctcgcga ccgccggcgc cggcatctcc gactcgaccg ggttctcgat
     1321 cacgaacgcg ggccgcaacc acctgcgcaa ctggcaggtg caataccagt ccgtcacgct
     1381 atccagcccg catatctatg gccaggtcac tcgcacgcac agcgacgcgg gcaagacgta
     1441 tcagctcgat cgcctcgcgc gcaccgtcgc tgcgatgggc ggcgtgccgg cgaatccggc
     1501 cgacttgaat gcgctgcgcg attcgatctc gctcgtcgac gacagccagc tcatcgactc
     1561 cgagctgcag taccgccaca cgctcgccgg cgtgaagacg acgctcggtg cgcagtatcg
     1621 tgcctacgcg ccgagctcgg cgggcacgta cctcgacgac aagacgcagg acatctcggt
     1681 cgccgaggcc ggcacgtact tgcagctcga cacgatgctg ttccatcgcc tgcgtctcgc
     1741 cggtgcggcg cgcgtcgatc accacacgct gtacgcgacg cagatcagcc cgaaggccgc
     1801 ggtgcagtac gagatcgcgg aaaaccagaa cgtacgcgtc ggctacaacc gtgcgttcaa
     1861 gagcccgacc gttctcgaga acttcttgcg catcaacgac gtgctgctcg gcaaccgcac
     1921 cggcttcgtc gtgcgcgatg caggtggcaa cgtgatcagc gacatcgcgc cgctgtcgcc
     1981 cgagcaggtc gacgcgatcg aagtcggcta caaggccgcg atcggcagca agctgtacgt
     2041 cgacgcagtc gcgtatcagt cgtggtaccg caacttcatc agcccgctga cgtcggtcgc
     2101 gaacccgatg gcggcgatgc cgacgttcgc gacgtacgcg gacggcactc cgacggccca
     2161 gggcacaccg gccgagggca cgctgtcgac gtacatgaac ttcggcaagg cgaaggtgcg
     2221 cggcatcgac gtcggcgtcg actacaagcc gatcgagcag ctcacgctgt cgacgagcgc
     2281 gtcggcgatg caactcgcga gcttcgagaa cacgaacccg ctgcagacgg atctgctgct
     2341 caacgcgcca gcgttcaagc tgcgcggctc ggtacagggc gatgacctcg gcctgaagaa
     2401 ctcgttctgg cgcgtcgata gccgctggca cacggcgtac gagttcgcgt cgggttactg
     2461 gaacagcatg acgctgctcg gcggcaaggt gccggcgcgc atggtgctcg acctctcgct
     2521 cggctacaag ctgccgaagc agggcatcac gatcagcggc acggtcgcga acctgctcga
     2581 caacaacgat ccggacgtgc tcggcgcgcc gattccgcgc cgcttcgcct ggctgcaggt
     2641 cgcgtacgac tgggacggtt tgcgctatta gccgacgttt gccgggcaac gccgcatggc
     2701 tcgcgtgtat caaacggcga ccatgcgcct cgccctgctt acgctcttgc tcatcgcgtg
     2761 caacaaagat cccgaacagc acatcgatgc gagcgtgcat ctgatcgatg ccaaatcggt
     2821 cgatgcgccg ccggatgcgc caccggacgc gccgcccgac gcggcaccac cgatgaacgt
     2881 cgttacggcg tgcatgcatg cgtgcgatgc gatcgcagcg tgcgtccaga tgccggccga
     2941 tcaggagtgc tacacggact gcgcgacgga cctggcggac tgcacggctc agcaggtcgc
     3001 gacgatcgac gcgtgcagca ccgagatgtg cggcgacctc gagaacaaca tgtcgccgct
     3061 gctcgactgc atcgtcgccg tgtcgtgcgt cgatatggcg acgatcagag gttcgcgtaa
     3121 ataaagcccg agcggctgtt gccgagctcg atccggtagg tgtgcaggtc gccgccggtc
     3181 gcgtgcatga agtgatcggt gtgctcgccc tggtcgacat cgtcgacgaa cacgcgcgcc
     3241 gtgcccttcc actcgagggt ggtgagcggt ccgacgacgt cgccgccgat cgcaacccac
     3301 tcaccgtcgc cgcgcgcgtg tagcgtgccg gcctcggggc cgccgacgca ggtcgcgcca
     3361 tcgcgcagcg ccgccaggat cgcgctcgcc gttgcatcga ctgcgagcac ccaggtggtc
     3421 gccatgacgt tcgtctggtg attgtcggtg ccgccgaccg ctgtcacccg gcgatgctcg
     3481 tcgtggacga cgcgattgag ctcggcccac gccagctctt cgccgctctt gccacccggg
     3541 cgcgagatca cgttcgcaat gccgagcggc acgttccaga cttcggcgcc gtcgatcgcc
     3601 gagaacccgg cgcggtgctg cgtccacacg cgataggaca tgtcgtaatg cgacgcgcgc
     3661 acgccgggga tcttcgtcgg cgtcgcgaat ggatggttga cgctgatcca cgcgccggct
     3721 gcatcggccg cagcgaggaa gtcgttgcct ttcagatcgg cgagattggc acccgcgacc
     3781 gtgaagtgac catcgcccgt cgtccactcg acaccgggaa tgagcgtcgg cgttttggcc
     3841 tccgcgcagc tcccggcgag ctcgcgccac gcccgttccc acttcgtgtg atcggtctgc
     3901 cagcgccacg gccacaggtg tggcgtgagc acgacgaagt ccatgccggc gcgcgctgcg
     3961 gcggccgcga tctcggtgac cgacagcatc acgtcatcgt gcgagtccgg cggcgagacg
     4021 tgcatgtgca tgtcacccgc gtaccaatgc cggcggtcct cgttcgcatc gaaccccgcg
     4081 gcaggcgcgt cgtcacgcgt cgcggcgcgc ggtgatgccg aagagcaggc aacgaccacc
     4141 aggacgaggg cgaggagccg cattgcccct tacgatccac gaccctgcat atcggtttac
     4201 gcggtcgcgc gaatcgggaa cggatccggg atatcgaggc cgagcgcggg gtcgccgagc
     4261 ggcatcgcga atagctccgc gtagatccac tcgaccaggc ccgggatgcc ggcccactcc
     4321 gcgccggccg cgaacgcctc gtgaacacgg tcgtgcaggg tgaggttctc gcgggtgtcg
     4381 gcgtccgcct cgacctggac cgcgcgcaac agcggggcct ccatcgtgtg cttggtcggc
     4441 gcgatcctgc tcgcggcaaa cgcgcgcgat ggcgcgaccg gcgggagcac gggccgcagt
     4501 gcatcgcggt gcaggtgcgc gaggtcgccg cgatccgcct tcgcgtacac ctgcgcctgc
     4561 gcgagcagta caagaaacgt ctcgcgatcg aacagaccgg gcagcacgtc gaccgggcgg
     4621 gcgcgccagt cgagcacgag gtgcgcgcta ttgccggtga gcgtgcgctc gacacgtcgg
     4681 ccgccgccga aatcgatcgt aacgatcggc accggacgta cggattccca gtgcagcacg
     4741 aattgctcgc ggagcacgcg gttgatttgc gtgtcggggt agagcagctt gcggaagaaa
     4801 cggctgttcg cgcacgatag ctcctcgtcg agccggccga gcaagcgcag cgacaagatc
     4861 ggccgcttcg tgcgccgcgc gtcgactagc gcagcgccct ggtcggtgtg ccagtagagc
     4921 agcgacgtgt gcgcgtcttt ttgcgcgcag acgcgatcga gcatcggcga cgcgctcgcg
     4981 ccgtagctgc ggagatacgt gtcgagggct tcgggcccaa cgcggcgaag gttctgcatg
     5041 acttcaccgt aaggcccgca gtgttagcgc aatgtaagga tacgagatcg gatacacgag
     5101 tgttttcagt cgcgtcaccg tgctgtcaaa cacggagccc agtgtcgtca catgtacaag
     5161 tcccttatcg tcctcattgc gctcagcggc agcgcacgtg cccagacgcc accggcggct
     5221 ccagaaacag ctaagacgcc aacggacacc gccgcggcag tcgaaaagcc cgctgaggct
     5281 ccaccaccga agaagctctc ggtcggaacc aaggggctct tcaatcccgg actcctcgcg
     5341 caagcgtggc tccaacacga tcgcgccggc gacacgacgc agatctcgca gttccgcctg
     5401 cgccgcgccg agctctccgt gagcggcgag atcatcccga agacggtggg ctacaaggtc
     5461 atgttcgatg ccgcgaaggt gcgcgagacg cagaaggtga cggttgcggg tccgcccgat
     5521 gcgatgggga atccaacgac ggtcaccatc aacaacccga cgagcgcggt ctcggcgctc
     5581 caggacttct acatcacggc gctgtcgccg ttcgtcgacg tgtcgatcgg tcagttcaag
     5641 atcccggtga gctgggaggg ctacaactcg tccggcaagc tgatgttccc cgagcgcgcc
     5701 gtcgtctcct cgacgttcgg cgacaagcgt gacctcggcg tgcggctcga gaagaagctc
     5761 gcgacggtcg gttactcggc gggtgtgttc aacggctcgg gactcgacaa cttcgacacc
     5821 aacgtgcaga aggatgtcgc gttgcgcctc gaggcgtatc cgatcaccgg cctgacggtc
     5881 gccggtgtca cgtatgactc tatcggccag cgcgaccatg ccggcacgaa ggatcgctgg
     5941 gaggcagatg tccgttacga ggccggcgac gtgctgctcc agagcgaagc gatcttcgcg
     6001 cgcgacgtga ccaaggatgc gatgcccgcg acgctcggtc gcggcttcta cgtgctcggc
     6061 gggtacacgc tgcacgacat cgattcgcag tttcacggcg atctgcagcc ggtggttcgc
     6121 gtcggagcgt tcgatccgga caccgatctg gccgacaacg cgctgctgca cttcgacgtc
     6181 ggcgtgaatt actatctcat caagcacgag atgaaggtcc aggccgcgta tcagcgcacg
     6241 cagttccagg attcgatgaa gcccgcaaac aacgagctga tccttgccgc gcaggtcatg
     6301 tactgatcag gcacgcccgg actagcgctc gatcggaaca cacgttcgca tgcagatgcc
     6361 gcacgtgcag gtggcaccac agtccgtgac gccggcatcg ccaccgcacg cgcagtagtc
     6421 gaccggcgtg tagttgcact cgcagaggcc ggcatcgttc ggcggctttt cttgcccagg
     6481 cgggttgggc gccggacacg catcgggatg gtcgtcgcta tgcgggcatg ccgcccagat
     6541 acagagcgcg aacaagcagc caattgcgag tcgtgggcgc gtcatgcgtt caccgcaggt
     6601 tggtcatcgt gagtcccttg tgactcggaa tgagcgcgag tccatcagga tcgatgaccg
     6661 tgtggttgtc cagcagatcg atcttcaggg cggcgccatc gggcacgccg tacagcgtga
     6721 ggttacgcga cgcgataagt gcggccaccc ctgcgaccgc gggtgcggcg aatgaggtgc
     6781 cggcggccga gatatccgca gcgtcggtgc ggccgttcag catgcccgga ccgccgatac
     6841 tgggccaatt acccggtgcc gcgatctccg acggctcacc gaagttggag tatgacgcga
     6901 tcccgccaag agacagcacc gagttgttcg tggcagcaac ggtcaaggat atctggcgtg
     6961 tcttcgtgcc cgcgaagaac gcctcgtccg gccagccgta gtagttcgtg accgagcctt
     7021 cgttgatccc gtcgtttgcg gtcgtccccc gtgtgctgcc gcgtcgtgcc cttcacttcg
     7081 tcggggccga cggagctacc gaacaggccg cctggatcct ggccgacgcc agcaaggcgt
     7141 ctgcttggtt cgccatcacg a
//
LOCUS       DASUFX010000534         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1058091, whole genome shotgun sequence.
ACCESSION   DASUFX010000534 DASUFX010000000
VERSION     DASUFX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058091"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFV99_21445"
     CDS             complement(<1..453)
                     /locus_tag="VFV99_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061954.1"
                     /translation="MNKLFTTVLPSVMVGALLFGSALSGAQPGTPVPPTPPTAPVPGS
                     PAMPPKPPKAPKLKGHINIDLGDLDQMVDEQIENALEAIGDNDQIPEHVRESLKKRLE
                     KVRVKVKARIGKLSPQDFDKMGEELGKMGDEIGAEMDQFGKDMDKWGKD"
     gene            complement(446..985)
                     /locus_tag="VFV99_21450"
     CDS             complement(446..985)
                     /locus_tag="VFV99_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061955.1"
                     /translation="MSSTTCDQVTERIALGEPLADLGEHAETCERCKRLVALPVELGA
                     IHREADPGLGFSARMTAGAQHRIVVRRRRRIAAGLAAAVAAGTLGVYAFTRQPASEQP
                     IANKTNPPTRDQATATNNQDKDPWANDASEDTDVDDDVRALVQLANTDTSSRYHADWK
                     AISKPLAPYRALIRGVGHE"
     gene            complement(982..1554)
                     /locus_tag="VFV99_21455"
     CDS             complement(982..1554)
                     /locus_tag="VFV99_21455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061956.1"
                     /translation="MASRNDARSRLLSELAGQPARKGYGIAYDLLGNRAEAEEAVQEA
                     LARACEAIGDLRDPAAAPAWFLRIVTTMCLRTLRRRKLKRTLFGWLPGKDEPEIAVDG
                     SSDAIAERMHASAGPVATEALVDREKLVAMFDRLDTLSSQQRAALVLRYGHDLPVATI
                     AEMLGVELATAKTHLVRGLARLRDLMEEHR"
     gene            1672..>3408
                     /locus_tag="VFV99_21460"
     CDS             1672..>3408
                     /locus_tag="VFV99_21460"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5061957.1"
                     /translation="MPRDLDRPFWRGQRPWFEIWFAVLLDENRRRALWIRETLFVPKE
                     GDGRATIWGAWFDADASPTTRTGKRWAPIETAKIGDPNASDELIRIDDAYIGKSGAAG
                     AVDELSWQAKWWGGKPVSDELPAWLPTPTHARQVLNDAETEATITVGNETIALRGRVL
                     AMHLWGKRRVPTLHWIWTPWLGDGALEVTAISLRDRFSLGLSSLTLDGPDKISGTPAT
                     AAHPHGLLTATVAGARRLMHAHAWAEIDDMVGYVYRDTDGSDLMVAQSDIGSAHFETF
                     SRTAPGAQWKLTEERRTAGGVAVEIHQRAPLPNVDYVGWDDTTRKPRPAKQAPRRPDQ
                     VEWPAVQSIVALGMTYADHIKETGGSVDPSVPPSAFMKHLRTLAPSGDARVPVPTTDE
                     LLAALAEVEPKLDVDIRKRMSIVPAVMDYEGELAVIALGDIDDERLAAGVAQPFGLAA
                     CNDLTARICQVFGEGMPRPEDYWACAKSFVRFLPVADRVWAPEGGLAKIPDLTLVTRV
                     NGEERQHASTKDLMYDLPAIVRVARMQLGRPLARGDVIITGTPSGIGMRLNVIRRRFA
                     KLVKDRFRKADFL"
BASE COUNT          552 a         1139 c         1128 g          589 t
ORIGIN      
        1 gtccttgccc cacttgtcca tgtccttgcc gaactgatcc atctcggcgc cgatctcgtc
       61 gcccatcttg ccgagctctt cgcccatctt gtcgaagtcc tgtggcgaga gcttgccgat
      121 gcgcgccttc accttcacgc gcactttctc gagacgcttc ttgagcgact cgcgcacgtg
      181 ctcgggaatc tgatcgttgt cgccgatcgc ctcgagcgcg ttctcgatct gctcgtcgac
      241 catctggtcg agatcgccga gatcgatgtt gatgtgaccc ttcagcttcg gcgccttcgg
      301 cggctttggc ggcatcgccg gcgaacccgg aacaggagcg gtcggcggcg tgggtggaac
      361 aggcgtaccg ggctgcgcgc ccgagagcgc gctaccgaac aacagagcac ctaccatcac
      421 ggacggcagg acagtggtga atagcttatt catgaccgac tcctcgaatc agagcgcgat
      481 acggcgcgag cggtttactg atcgctttcc agtcagcgtg gtaccggctc gacgtatcgg
      541 tattggcgag ctggacgagc gcacgcacat cgtcgtcgac gtccgtgtcc tcggatgcgt
      601 cgttcgccca cggatccttg tcctggttgt tcgtcgccgt cgcctgatcg cgcgtcggcg
      661 gattcgtctt gttcgcgatc ggctgctccg acgccggctg gcgcgtgaac gcgtagactc
      721 cgagcgtgcc cgctgcaacg gcagctgcga gccccgcggc gatgcgccgg cggcgacgca
      781 ccacgatgcg atgctgtgca cctgctgtca tgcgcgccga gaagccgagg cccggatccg
      841 cttcgcgatg gatggcaccg agctcgaccg gtagtgcgac gagacgcttg cagcgttcac
      901 acgtttccgc gtgctcgccg aggtctgcga gtggctcacc gagcgcgatg cgctcggtga
      961 cttgatcaca agtggtactg ctcatcggtg ctcctccatc agatcgcgca ggcgcgcgag
     1021 tcctcgaacc agatgcgtct ttgcggtcgc gagctcgacg ccgagcattt cggcgatcgt
     1081 tgcgaccggc aggtcgtgtc cgtagcgcag cacgagtgcc gcgcgctgtt gcgatgacaa
     1141 cgtgtcgagc cgatcgaaca ttgcaaccag cttctcgcga tcgacgagcg cttcggtcgc
     1201 gaccggccca gccgatgcat gcatgcgctc tgcgatcgcg tcgctcgaac cgtcgactgc
     1261 gatctcgggc tcgtccttgc cgggcagcca gccaaacagc gtgcgcttca gcttgcgacg
     1321 ccgcagcgtg cgcaaacaca tcgtcgtgac gatgcggaga aaccacgccg gtgcggccgc
     1381 cggatcgcgc aggtcaccga tcgcctcgca tgcgcgcgcg agcgcctcct gcacggcttc
     1441 ttcggcttcg gcgcggttgc cgagcaggtc gtaggcgatg ccataaccct tgcgcgccgg
     1501 ttgaccggcg agctcggaga gcaagcgact tcgggcgtcg ttccgggatg ccacgtctag
     1561 ccgcgctccg gtcgtaaggg acatatttgc cacgctggtc ttaagagcct cgacgtagct
     1621 gtgcggtgga cacatatagc gcggcccggc caatcgatgt atgtgaggga gatgcctcgg
     1681 gatctcgacc gaccattttg gcgcggccag cgcccctggt tcgagatctg gtttgcggtg
     1741 ctcctcgacg agaatcgacg ccgtgcgttg tggatccgag aaaccctgtt cgtgccgaaa
     1801 gagggcgacg ggcgggccac gatctggggc gcgtggttcg acgccgatgc ctcgccgacc
     1861 acgcgcaccg gcaagcgctg ggcaccgatc gagaccgcaa agatcggcga cccgaacgcc
     1921 agtgacgagc tgattcgcat cgacgacgcg tacatcggca agagcggcgc tgccggcgca
     1981 gtcgacgagc tgtcgtggca agcaaagtgg tggggcggca agcccgtcag cgacgagcta
     2041 cccgcgtggc tgccgacgcc cacccacgcg cgccaggtac tcaacgacgc cgagaccgaa
     2101 gcaacgatca cggtgggcaa cgagacgatc gcgctgcgcg gccgcgtgct cgcgatgcat
     2161 ttgtggggca agcgccgcgt accgacgctg cactggatct ggacgccgtg gctcggcgac
     2221 ggtgcactcg aggtcacggc gatctcgctg cgcgatcggt tctcgctcgg attgtcgtcg
     2281 ctcacgctcg acggtccgga caagatctcc ggcacgcccg ccaccgctgc gcacccgcac
     2341 ggcctgctca ccgcgacggt cgcaggtgcg cgccgcttga tgcacgcgca cgcgtgggcc
     2401 gagatcgacg acatggtcgg ttacgtgtac cgcgataccg atggcagcga tctgatggtc
     2461 gcgcaaagcg acatcggctc ggcacacttc gagacattct cgcgcaccgc accgggcgcg
     2521 cagtggaagc tcaccgagga gcggcgcacc gcgggcggcg tcgccgtcga gattcatcag
     2581 cgcgcgccgc taccgaacgt cgactacgtc ggctgggacg acacgacgcg caagccgagg
     2641 ccggcgaagc aggcgccgcg tcgtcccgat caggtcgagt ggcctgcggt gcaatcgatc
     2701 gtcgcgctcg gcatgacgta cgccgatcac atcaaggaga ccggcggctc ggtcgacccg
     2761 agcgtgccac cctcggcgtt catgaagcac ctgcgcacgc tcgcgccgag tggcgatgcg
     2821 cgcgtgccgg tgccgacgac cgacgagctc ctcgccgcgc tcgccgaggt cgagccgaag
     2881 ctcgatgtcg acattcgcaa gcgcatgtcg atcgtgcccg cggtgatgga ctacgaaggc
     2941 gagctcgcgg tgatcgcgct cggcgatatc gacgacgaac ggctcgctgc gggcgtcgcg
     3001 caaccgttcg ggctcgccgc gtgcaacgat ctcaccgcgc gcatctgtca ggtgttcggc
     3061 gaaggcatgc cgaggcccga ggattactgg gcgtgcgcga agtcgttcgt gcgcttcttg
     3121 cccgtcgccg atcgcgtgtg ggctcccgag ggtggcctcg caaagattcc cgacctcacg
     3181 ctcgtcacgc gcgtcaacgg cgaggaacgc cagcacgcct cgaccaaaga tctgatgtac
     3241 gacctgccgg cgatcgtgcg cgtcgcacgc atgcagctcg gtcgaccgct cgcgcgcggc
     3301 gacgtgatca tcaccggcac gccgtcgggc atcggtatgc ggctcaacgt gatccgccgc
     3361 cggttcgcga agctcgtgaa ggatcgcttc cgcaaggccg acttcctg
//
LOCUS       DASUFX010000535         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_503571,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000535 DASUFX010000000
VERSION     DASUFX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503571"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..983)
                     /locus_tag="VFV99_21465"
     CDS             complement(123..983)
                     /locus_tag="VFV99_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5061958.1"
                     /translation="MRLIIVPVANRPECQVALDVAFGLAAELGADVAGYHIRPVHREQ
                     SLALGPLLPDDDVHASFAVADSKAGSGLKAARRIHERVAAKHGFALAERPARGRRMRA
                     FWHEVAGTPARVFAVVGPVADLTVVSRPRAEGASRARAFLLGALLHSAKPVLVMPQAA
                     HAATVGKRIVIAWNQSADAAAAVTAALPLLTRAERVVVVSAGRENRLGPSSTQLARYL
                     AHWDVSVERVRTKGQHVERELERACRDLDADLIVMGAYSRSRFRQLVFGGVTEHMLFK
                     TNLPVLMLHR"
     gene            complement(988..1317)
                     /locus_tag="VFV99_21470"
     CDS             complement(988..1317)
                     /locus_tag="VFV99_21470"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5061959.1"
                     /translation="MNVNASLIVTIVRKGWGSSVLQASVKAGARGGTVLLGRGAGVHE
                     HEKIFGMSIEPEKEIVLTIVQSDQVEAILGEIVRAAELDAAGHGMAFVVPVHEIVGVA
                     DSMLLGA"
     gene            complement(1332..2054)
                     /locus_tag="VFV99_21475"
     CDS             complement(1332..2054)
                     /locus_tag="VFV99_21475"
                     /inference="COORDINATES: protein motif:HMM:NF019194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX5061960.1"
                     /translation="MNDVPLLADLVRTARGVALAVLPLAGLFAVFQLLFLRLPRTEIA
                     RIATGTVMASIGLFLFLLGISVGFMPFGRTVGQTIGAISQTWLLAIFGALLGFATTWG
                     EPAVRILADQVEQASTGTIRRSVVLIAVCTGVAVAVAIGMLRITYDWPLFWILAPGYA
                     AAIALIWLSDRSFVAIAVDAGGVATGPLANTFLLALGFGVAAAVAGRDPLVHGLGLAA
                     LIALAPIISVMLLGFAVRLKSA"
     gene            complement(2051..2746)
                     /locus_tag="VFV99_21480"
     CDS             complement(2051..2746)
                     /locus_tag="VFV99_21480"
                     /inference="COORDINATES: protein motif:HMM:NF019194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX5061961.1"
                     /translation="MITLLKLKLAETLRAVAPLIVVVSVLQVTVVGASAAVFLQYLAG
                     SVLVTAGLLLLLTGIDFGILPMGRYIGAKLAEKSSLSLLLIVAFALGFATTAAEPDVL
                     VLASMVGDASQGELPSQPMVYVIAAGVGLFTVVGFVRVVLGLSMRWQLTAMYALMVAL
                     TFLAPSAVVPLAYDAGSVTTGVLAGPVVLAVALGVSSVIAGRSAVHDAFGLLGLASIG
                     PIIVILTIGLMTR"
     gene            complement(2743..>3057)
                     /locus_tag="VFV99_21485"
     CDS             complement(2743..>3057)
                     /locus_tag="VFV99_21485"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5061962.1"
                     /translation="MAGTVDLVQRVSTGLEMTGNVLRLNPRLPEGLERLDMRIRYRGH
                     TLDLTITRDELRVRDRERGPAPIELAYRDEVCELVGGSARVFALTPPIPVAPDMPHEL
                     DK"
BASE COUNT          593 a         1041 c         1000 g          423 t
ORIGIN      
        1 tacgaaagga acgcgcggag attaggcacg agagcaagcg ttggattggg catgggcctt
       61 gcgcagctcc atgcttcggc attgtggtcg gctccgcggc gtgcggggcg aaacggccgg
      121 cgtcatcgat gcaacatcag cactgggaga ttggttttga agagcatgtg ctcggtgacg
      181 ccgccgaaga cgagctgccg gaaccggctg cggctgtagg cgcccatcac gatgagatcg
      241 gcgtcgagat cgcgacacgc ccgctcgagc tcacgctcga catgctgccc cttcgtgcgc
      301 acgcgctcga ccgacacgtc ccaatgtgcg aggtatcgag ccaattgcgt gctgctcggc
      361 cccaagcggt tctcgcgccc cgcgctgacg acgacgacgc gttcggcacg cgtgagcagc
      421 ggcaacgcgg ccgttaccgc cgccgcggcg tccgcgcttt ggttccaagc gattacgatg
      481 cgcttgccga cggtcgccgc gtgcgcggcc tgcggcatca ccagcaccgg tttcgcggag
      541 tgcagcagag ctcccaacaa gaacgcgcga gcgcggctgg ccccctccgc cctgggtcgc
      601 gacacgaccg tgaggtccgc aacggggcca acgaccgcga acacgcgtgc cggggtgccc
      661 gcgacctcat gccaaaacgc gcgcattcgc cggccgcgcg ccggccgctc cgccaaggcg
      721 aagccgtgct tggcggcgac gcgttcgtgg atgcgccgag ccgctttcaa acctgaaccc
      781 gccttggagt ccgcaacggc aaacgacgcg tgcacgtcgt cgtcgggtag taaggggccg
      841 agcgcgaggc tctgctctcg atgaacgggg cggatgtgat agccggcaac gtccgcgccg
      901 agctcagccg cgagcccgaa cgcgacgtcg agcgcgacct ggcattcagg acgattggcg
      961 acgggaacga tgatcaggcg cacgctccta agctccgagc agcatcgaat cggccacgcc
     1021 gacgatctcg tgcacgggaa cgacgaacgc catgccgtgg ccggcggcgt cgagctcggc
     1081 cgcccggacg atctcaccta agatcgcttc cacttgatcg ctctggacga tcgtgaggac
     1141 gatctccttc tcgggctcga tcgacatgcc gaagatcttc tcgtgctcgt ggacgccggc
     1201 cccgcgcccg aggagcacgg tgccgccgcg cgcccccgcc ttcaccgaag cttgcaggac
     1261 gctgctgccc caccctttgc ggacgatggt gacgatgagc gaagcgttga cgttcacggt
     1321 tcgacctcct ctcatgcgct cttcaaacgg acggcgaaac ccaacagcat caccgagatg
     1381 atcggcgcga gcgcgatgag cgccgcgaga ccgagcccat gcacgagcgg atcgcgaccg
     1441 gccacggcgg cggcgacgcc gaaaccgagc gccagcagaa acgtattggc gagcgggccg
     1501 gtggcgacac cgcccgcatc gaccgcgatc gcgacgaagg agcggtcgct gagccagatc
     1561 agggcgatcg ctgccgcgta gcccggagca aggatccaaa agagcggcca gtcgtaggtg
     1621 attctcagca ttccgatggc gacggcgacg gcgacgccgg tgcacacagc aatgagcacg
     1681 acgctccgac gaatcgtgcc cgtcgatgcc tgctcgactt gatcggccag gatgcgcacg
     1741 gcgggctcgc cccaggtggt ggcgaaaccg agcaaggcgc caaagatcgc gagcagccac
     1801 gtctgcgaga tggcgccgat ggtctgcccg acggtgcgtc cgaacggcat gaaacccacg
     1861 ctgatgccga gaagaaagag aaatagtccg atcgacgcca tcaccgtacc cgtcgcgatg
     1921 cgcgctattt ccgtgcgcgg caggcgcaga aacaagagct gaaacaccgc gaataacccc
     1981 gcgagcggca gcacggcgag cgcgacccct cgggcggtgc gaacgagatc ggccagcagc
     2041 ggtacgtcgt tcatcgggtc atcaagccaa tcgtcaggat cacgatgatg ggcccgatcg
     2101 atgccaggcc caagaggccg aacgcgtcgt gcaccgccga ccggcccgcg atgaccgagc
     2161 tcacgccgag ggcgaccgcc agcacgactg ggccagccag cacgcctgtc gtcacgctgc
     2221 cggcatcgta cgcgagcggg accaccgcgc tcggcgcgag aaaagtcagc gcgaccatca
     2281 acgcgtacat cgccgtcaac tgccatcgca tcgacaagcc cagcacgacg cgcacgaacc
     2341 cgacgacggt gaagagcccg actcccgctg cgatgacgta taccatcggc tggctcggga
     2401 gctcgccttg cgaagcgtcg cccaccatgc tcgcgaggac cagcacgtcg ggctccgccg
     2461 ccgtcgtcgc gaacccgagc gcgaaagcta cgatcaatag caacgacaac gagcttttct
     2521 ccgccagctt cgctccgatg tagcggccca tcggcagaat gccgaaatcg attcccgtca
     2581 ggaggagcag caacccggcg gtgacgagca cggagcccgc gagatactgc aggaacaccg
     2641 ccgccgacgc gccaacgacc gtgacttgca ggacagagac gacgacgatc agaggcgcga
     2701 cggctcgcaa cgtctcggca agctttagct tgagcagcgt gatcacttgt cgagctcgtg
     2761 cggcatatcc ggcgcgacgg ggatcggcgg cgtcagcgcg aacacccgcg cgctaccgcc
     2821 gacgagctcg catacttcgt cgcggtacgc gagctcgatc ggcgcgggac cgcgctcgcg
     2881 gtcgcgcacg cgaagctcat cccgcgtgat cgtaagatcg agcgtgtggc cgcgatagcg
     2941 gatgcgcatg tcgagtcgtt ccagtccttc aggcaagcgc gggttcagcc gcaggacatt
     3001 acccgtcatc tcgagtcccg tcgatacgcg ctgcaccagg tcgaccgtgc cggccat
//
LOCUS       DASUFX010000536         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1753049, whole genome shotgun sequence.
ACCESSION   DASUFX010000536 DASUFX010000000
VERSION     DASUFX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753049"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFV99_21490"
     CDS             <1..445
                     /locus_tag="VFV99_21490"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEX5061963.1"
                     /translation="QANVAYALVPADDPRIADYMARLDEINQLADASPGFVWRYLTDS
                     RDPTQREFSDPFVLFNMSVWESIDALHAYTYRTAHAEVYAARKRWFVDQKAVVGGHAL
                     AMWWIPAGEWPTVADAKERLAAIVANGPSERAFTFKQRFPPPTPA"
     gene            complement(459..1295)
                     /locus_tag="VFV99_21495"
     CDS             complement(459..1295)
                     /locus_tag="VFV99_21495"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061964.1"
                     /translation="MRNVGVLLAVAACGSESGTGNTIDAATTGDATAADASGQVDAAL
                     ALCARSGLVFCEDFETLPLGPATSSTWTTESASGQLAIDATHARGQHALKVSTTGNGR
                     ARLQKMLALPSNSAWGTMHAWVSAFPTAPDYAHYTLVELAGTGNTSLVRPIGGQYAPA
                     AGSNNPAGAFWGVGSDAGATGDWTNWKRSAPSMGGRWLCLEFHLDATNDAIDIYLDGV
                     AHPELSVTRTSHGGNQVDFVFPTFNKIWFGWWLYQTNPTPGTYDVWLDDLALGSTRLG
                     CD"
     gene            1323..1583
                     /locus_tag="VFV99_21500"
     CDS             1323..1583
                     /locus_tag="VFV99_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061965.1"
                     /translation="MVDRHLFVMSQMIIQLSGAVVILAAFVLAQARVLRVEQRLYLLL
                     NAVGASVLAVNAYTERQWGFLLLEGVWALVAAWGLLRPRPAR"
     gene            complement(1584..2195)
                     /locus_tag="VFV99_21505"
     CDS             complement(1584..2195)
                     /locus_tag="VFV99_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061966.1"
                     /translation="MLIAVERLLLKLRQPASRADELVELRRRLRAELASDVDETERAL
                     AREVHARKLDVTAELRAVSSCASCATGQPWPVGHYDGGACCSGVTANLFDENELAALV
                     HAGTRTSDLTPPKDAHAGCAFRGSHGCTLEVEHRPARCVHYVCNTLRRELFDRGQLDA
                     VEGKLAALDHAMQRFSAVHRARLDREVADSVLAAVGAAAKSRP"
     gene            complement(2216..3697)
                     /locus_tag="VFV99_21510"
     CDS             complement(2216..3697)
                     /locus_tag="VFV99_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061967.1"
                     /translation="MTKIRLVTSSSLLVLGLASACSTSDDVWYPPGSEGGKADAFTTI
                     KGSDIPSQYVSASKSYLIARRIDSLKTAGALDMVEAPLADRIDGIIANMPDDGKLHLA
                     ELVRMEDPAIFDSLFAAEAAALPKMWAKMEAPSTNDLVTGPDVGFGVLDMASPPGPAV
                     PPASLAISSLATTDLQNAATRLQNVYNADNNTSTVQIADLDSGVANPGAFTQAEVTAF
                     GTIKAVFREKAVAVADATLVLSAGPGPFSSNATLGPIGLQISGTTRIEEERNHYSSTL
                     TLRLTAIQNQTAMATLPQGAQVLVINKDSANEVVFGSGAVPSLVAGPHVVEVWNGGQR
                     VFVTNAVLPATTKEQRIDLTDKLDYTVTTALAPLVRNVVSATYTGSQFNAKFSYDKTA
                     VQPMVAPNATALQRVATPKVNIPVGRYTFSNVQTQLLVFPNDVLWIQRGNAMYRLLPQ
                     GNGGVPTRFYNNQINAYFETTNNSFYMSSPSFSTVLNASMRDI"
     gene            complement(3861..>4202)
                     /locus_tag="VFV99_21515"
     CDS             complement(3861..>4202)
                     /locus_tag="VFV99_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061968.1"
                     /translation="VGVLVIVLMTLGNAAANMIPFHVPTARELQRAEQAATADARRAR
                     IAALQAEGDRCRAEIARELARALVFDGQSAVAYADDYARRCGEDPIVRRWGDASLRLV
                     SLHGARRSRHE"
BASE COUNT          695 a         1391 c         1386 g          730 t
ORIGIN      
        1 acaggcgaac gtcgcgtacg cattggttcc tgcggacgat ccgcggatcg ccgattacat
       61 ggcgcgcctg gacgagatca atcagctcgc ggatgccagt ccgggcttcg tgtggcgcta
      121 cctcaccgat tcacgcgacc ccacgcaacg cgagttctcc gatccgttcg tgctcttcaa
      181 catgtcggtg tgggaatcga tcgacgcgct gcacgcgtac acgtatcgaa ccgcgcacgc
      241 cgaggtctac gccgcgcgca agcgctggtt cgtcgaccaa aaggcggtcg tcggtggtca
      301 cgcgcttgcg atgtggtgga ttccggccgg cgagtggccg accgtcgccg atgccaagga
      361 gcgtctcgcc gcgatcgtcg caaatgggcc gagcgaacgc gcattcacgt tcaagcagcg
      421 ctttccgcca cctaccccag cgtagatagc gcgccggatc agtcgcagcc gaggcgcgtg
      481 gagccgagcg cgagatcgtc gagccacacg tcgtaggtgc ccggcgtcgg gttcgtctgg
      541 tacagccacc agccaaacca gatcttgttg aacgtcggaa acacgaagtc gacctgattg
      601 ccgccgtgac tcgtgcgcgt caccgaaagc tcgggatgcg cgacgccatc gagatagatg
      661 tcgatcgcat cgttcgtcgc atcgaggtgg aactcgaggc acagccacct cccacccatc
      721 gacggcgcgc tgcgcttcca gttcgtccag tcgccggtcg cgcccgcatc actgccgacg
      781 ccccagaatg cgccggcggg attattgctg ccggctgcgg gcgcgtactg gccaccgatc
      841 ggccgcacga gtgacgtgtt accagtgccg gcaagctcga cgagcgtgta atgcgcgtag
      901 tccggcgcgg tcgggaacgc ggacacccac gcgtgcatcg ttccccatgc gctgttgctc
      961 ggcagcgcga gcatcttctg cagccgcgcg cgaccgttac ccgtcgtcga gactttgagc
     1021 gcgtgctggc cgcgcgcatg cgtcgcgtcg atcgcgagct ggccgcttgc cgactcggtc
     1081 gtccacgtgg agctcgtcgc cggcccgagc ggcagcgttt cgaagtcttc gcagaacacg
     1141 aggccactac gcgcgcacag cgcgagcgca gcatcgacct gcccgcttgc atcggcagcc
     1201 gtcgcatcgc cggtggttgc ggcgtcgatc gtgttaccgg tcccactctc cgacccgcac
     1261 gcagcgacag cgagcaagac gccgacgttc cgcatcccgt gatgctaccc aagtgctagg
     1321 gtgtggtcga caggcatctg ttcgtcatga gtcagatgat cattcagctg tcaggcgccg
     1381 tggtcattct cgcggcattc gtgctcgcgc aagcccgcgt gctgcgcgtg gagcagcgcc
     1441 tgtacctcct cctgaacgcg gtcggtgcgt cggtgctcgc cgtcaacgcg tacaccgagc
     1501 ggcaatgggg cttcctgctc ctcgaaggtg tatgggcact cgtcgccgcg tggggcctac
     1561 tccgcccgcg ccccgcgcgc tgactacggc cggctcttcg cagccgcgcc aacggcagcg
     1621 agtactgaat cggcaacctc gcgatcgaga cgcgcgcgat gcaccgcgga gaaccgctgc
     1681 atcgcatgat cgagggcggc gagcttgccc tcgacagcat cgagctgccc gcggtcgaac
     1741 agctcgcggc gtagcgtgtt gcagacgtaa tgcacgcagc gcgcaggacg gtgctcgacc
     1801 tcgagcgtgc agccgtgact gccgcgaaac gcacatcccg cgtgcgcgtc tttgggcggc
     1861 gtgagatcgc tcgtccgcgt tcccgcgtga accagtgcag cgagctcgtt ctcgtcgaac
     1921 agatttgcag tcacaccgga gcagcacgcg ccgccgtcgt agtgaccgac cggccacggc
     1981 tgcccggtcg cgcacgacgc gcacgacgac accgcacgca gctcggcagt gacgtcgagc
     2041 ttccgcgcgt gaacctctcg cgcgagcgcg cgttcggtct cgtcgacgtc gctagccagc
     2101 tcggcgcgca accgccgccg cagctcgacg agctcgtcgg cccgcgacgc cggctggcga
     2161 agcttgagga ggagccgctc gacggcgatc aacactcgcc gagcctagca tcgagttaga
     2221 tgtcgcgcat cgaggcgttc agcacggtgc tgaacgacgg cgagctcatg tagaacgagt
     2281 tgttcgtggt ctcgaagtac gcgttgatct gattgttgta gaagcgcgtc ggcacgccgc
     2341 cgttgccttg cggcagcagg cggtacatcg cgttgccgcg ctggatccac agcacgtcgt
     2401 tcgggaagac gagcagctgg gtctggacgt tcgagaacgt gtaacggccg accgggatgt
     2461 tcaccttcgg cgtcgcgacg cgctggagcg cggttgcgtt cggcgcgacc atcggctgca
     2521 ccgcggtctt gtcgtagctg aacttcgcgt tgaactgcga gcccgtgtag gtcgccgaga
     2581 ccacgttgcg cacgagcggc gcgagcgccg tcgtcaccgt gtagtcgagc ttgtcggtga
     2641 gatcgatgcg ctgctctttc gtcgttgcag ggagcacggc gttcgtcacg aacacgcgct
     2701 ggccaccgtt ccagacctcg acgacgtgag gaccagcgac aagcgacggc acggcgcccg
     2761 agccgaacac gacctcgttc gcgctgtcct tgttgatcac gaggacctgg gcgccctgcg
     2821 gcagcgtcgc catcgcggtc tggttctgga tcgcagtgag gcgaagcgtc agcgtcgagc
     2881 tgtagtgatt gcgctcttct tcgatgcgcg tcgtcccgga aatctgtaga ccgatcgggc
     2941 cgagcgtcgc attgctcgag aacggtccgg ggccggccga gagcacgagc gtcgcgtcgg
     3001 caacggcgac cgccttctcg cggaagaccg ccttgatcgt gccgaacgcg gtgacttcgg
     3061 cctgcgtgaa cgcgccgggg ttggcaacac cgctgtcgag gtccgcaatc tgcacggtcg
     3121 acgtgttgtt gtcggcgttg tagacgttct gcagtcgggt cgctgcgttc tggagatccg
     3181 tcgtcgccag cgaggagatg gcgagcgatg cgggcggcac ggccgggccc ggaggcgacg
     3241 ccatgtcgag gacgccgaag ccgacgtccg gaccggtcac gagatcgttc gtcgacggcg
     3301 cttccatctt tgcccacatc ttcggcagcg ccgcagcttc cgccgcgaac aggctgtcga
     3361 agatcgccgg atcctccatc cgcacgagct cggcgagatg cagctttccg tcatcgggca
     3421 tgttcgcgat gatgccgtcg atgcgatcgg cgagcggcgc ttcgaccata tcgagcgcgc
     3481 cagccgtctt cagcgaatcg atacggcggg cgatcagata cgacttcgag gcgctgacgt
     3541 actgcgacgg gatgtcgcta cctttgatcg tggtgaacgc atcggcctta ccgccttccg
     3601 agcccggcgg ataccagacg tcgtcggagg tggagcatgc cgaggcaaga ccgagcacga
     3661 gaagtgaact agaagttaca aggcggatct tcgtcatagc tggtgcgccg caaagcaagc
     3721 tctaggcctc gcaccacgcg aatggtcgta ccaacttgct cgttcacgcc gtggcgagtc
     3781 gagttgccat cggccactga aatgaccagc ccgacgttgt cagcgcattt catgcgaccg
     3841 gtgaaacgcg ccacgcgcgc tcattcgtgg cgcgatctgc gcgcgccgtg cagtgacacc
     3901 agtcggaggc tcgcgtcgcc ccagcgccgc acgatcggat cttcgccgca gcgtcgcgcg
     3961 tagtcgtcgg cgtacgcgac ggccgattgg ccatcgaaca cgagcgcgcg tgccagctcg
     4021 cgcgcgatct cggcgcggca tcgatcaccc tcggcttgca acgcggcgat gcgcgcacga
     4081 cgcgcgtcgg ctgttgccgc ctgttcggcg cgctggagct cgcgcgcggt cggcacatga
     4141 aacgggatca tgttcgccgc agcgttgccg agcgtcatga gcactatcac cagaacaccg
     4201 ac
//
LOCUS       DASUFX010000537         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_364014,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000537 DASUFX010000000
VERSION     DASUFX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364014"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFV99_21520"
     CDS             complement(<1..349)
                     /locus_tag="VFV99_21520"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061969.1"
                     /translation="MRKLARGGMAELFLARTIGPEGFEKLVVLKKILPTHAENPKFVR
                     LFLDEAKLAATLDHPHIAHVYDMGKVDGNYFFTMEYVHGQDVRTTMRRTTRLDQKLPI
                     DHAVQVARNVAAAL"
     gene            575..>2245
                     /locus_tag="VFV99_21525"
     CDS             575..>2245
                     /locus_tag="VFV99_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061970.1"
                     /translation="MAACAAEDDKAPGEWAPGKGDGAFELVEVGPAPLDARVDIALDH
                     RVFAYRVESYGGTKLKIDLKGRSTDAYLIVEGPLAGDGDRLAIGAGTVIGEDDDSGYG
                     RDARLELELAEPGVYRILTGTYESLGEGGAASGSLTLTVACSANCSRPMVDQKNFVRA
                     LQQQAGPAFTEYAKAELTSLVHDPAIAEQMTAQLTAILADPQLTGLERFPTIPLAAIG
                     ALRPALGQIPAQPASEDKVVTGDLMQLLGGCTPDRSLPAEVDARLPGVRYGQFPSKTL
                     SPCQFAHASKLAQVLTSLAAQNGSSVTFKGQTIKTPRELFAALIASGHTIEVRNERMY
                     ANFLSMIAGDRDVIWPVWLDTGIKLSNGESLSIPVGHSHHAWRITGPNINTRVMFYLG
                     ISGAGFFGQTDQRPAWSGTVASTDITVTAAQGADHDYLLKTADAAATYLRRNRVERTT
                     VAQGMPADGYGYVGVCNDSNAAIEHATKGTITAFPLLRAKELDAQADLGDGLDATIKA
                     LPKDGDGIANQRDALRRAVAMQPFANGSPLIWDPKLAAQLATARADLGQ"
BASE COUNT          391 a          734 c          739 g          381 t
ORIGIN      
        1 gcaaggcggc cgcaacgttg cgcgcgacct gcacggcgtg atcgatcggc agtttctgat
       61 cgagacgcgt cgtgcgacgc atcgtcgtgc gcacgtcctg cccgtgcacg tactccatcg
      121 tgaagaagta gttcccgtcg accttgccca tgtcgtagac gtgagcgatg tgcggatggt
      181 cgagcgttgc agcgagcttc gcctcgtcga gaaacagccg aacgaacttc gggttctcgg
      241 cgtgcgtcgg caaaatcttc ttcagcacga cgagcttctc gaagccttca ggaccgatcg
      301 tgcgtgcgag gaagagctcc gccatcccgc cacgagcgag cttgcgcaca acctcataag
      361 aaccgagacg catagtctcg gcccgtgctt gatctcccgg cgtccccaag cgcttcgatg
      421 atatcgcgtt gacacatcgc gtggcttgtt tcctcgacgg catccctact agccagtgtg
      481 ttgcaagctc gtgggatgac ttgcgcattt cgcggcacaa cgagtgcaaa caccagtgcg
      541 catgcgtact cgactggctt gtgcggttgt cctcgtggct gcgtgtgctg cggaggatga
      601 caaggcgccg ggcgaatggg ctccgggcaa aggcgatggc gccttcgagc tcgtcgaggt
      661 tggaccagcg ccgctcgatg cgcgcgtcga catcgcgctc gatcaccgcg ttttcgcgta
      721 ccgcgtcgag tcgtacggcg gcacgaagct gaagatcgac ctcaaaggtc ggagcactga
      781 cgcgtatttg atcgtcgagg gtccgcttgc gggcgatggc gatcgcctcg ctatcggcgc
      841 cggcacggta atcggtgagg atgatgacag tggttacggt cgcgacgcgc ggctcgagct
      901 cgagctcgca gaacccggcg tgtatcgaat cctgaccggc acctacgaga gcctcggcga
      961 aggcggtgcc gcgagtggtt cgctcacgct gaccgtcgcg tgcagtgcga actgctcccg
     1021 gccaatggtc gaccagaaga acttcgtgcg cgcactccag cagcaagcgg ggccggcgtt
     1081 caccgagtac gcgaaggccg agctcaccag cctcgtccac gatcccgcga tcgccgagca
     1141 gatgacggcg cagctgaccg cgatcctcgc cgacccgcag ctcaccgggt tggagcgttt
     1201 cccgacaatc ccactggccg cgattggcgc gttgcgcccc gcgcttgggc agattcctgc
     1261 gcaaccggcg agcgaggaca aggtcgtgac cggcgatctg atgcagctac tcggtggctg
     1321 cacgccggat cgttcgctgc ccgccgaagt cgacgcgcgg ctgcccggcg tccgctacgg
     1381 ccagttcccg agcaagacgc tatcgccgtg tcagttcgcg catgcgagca agctcgccca
     1441 ggtgctgacg tcgctcgcgg cgcagaacgg ttcgtcggtg acgttcaagg gccagacgat
     1501 caagacgccg cgcgagttgt tcgcggcgct gatcgcgagc ggtcacacga tcgaggttcg
     1561 caacgagcgc atgtacgcca acttcctgtc gatgatcgcc ggcgaccgcg acgtgatctg
     1621 gccggtgtgg ctcgacaccg ggatcaagct gtcgaatggc gagagcctgt cgattccggt
     1681 cggccacagc caccacgcgt ggcgcatcac cggcccgaac atcaacacgc gcgtgatgtt
     1741 ctatctgggc atctcgggtg cgggcttctt cggccagacc gaccagcgtc cagcgtggtc
     1801 cggcacggtc gcatcgaccg acatcaccgt gacggcggcg cagggcgccg accacgacta
     1861 tctattgaag acggccgacg ctgccgcgac gtatctgcgc cgcaaccgcg tcgagcgcac
     1921 gacggtcgcg caaggcatgc ccgccgacgg ctacggctac gtcggcgtct gcaacgattc
     1981 gaacgcagcg atcgagcacg cgacgaaggg cacgatcacc gcgttcccgc tcctccgtgc
     2041 gaaggagctc gacgcgcagg ccgatctcgg tgacgggctc gacgcgacga tcaaggcgct
     2101 gccgaaggac ggcgatggca tcgcgaacca gcgggacgcg cttcgccgtg ccgtcgcgat
     2161 gcagccgttt gcgaacgggt cgccgctgat ctgggatccg aagcttgccg cccagctcgc
     2221 gacggcgcgg gccgacctcg ggcag
//
LOCUS       DASUFX010000538         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_364023,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000538 DASUFX010000000
VERSION     DASUFX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364023"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VFV99_21530"
     CDS             complement(<1..1018)
                     /locus_tag="VFV99_21530"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5061971.1"
                     /translation="MAEERTRALVGECISRAHVEAARSATIDAITEACEACDYETAAT
                     MVLGRARAGEHVPAPLVARVLPGIELPAITLALVAIVAKDDRPKLLELVENRRFPHTK
                     DACELEAILVFAAWRAEAPTERVIPELRRLSARPMSVESYALLATIAASIDDVNVAAA
                     TKPIAPFAKEYAKQVSADEKAMNASLEAVIASLPAEIETTRATGFTVRAAKQVGRNDP
                     CPCGSGLKYKKCHADKDEARTASASPVAGLSWDEFLAGDKLTATHAADLALRDLVRVG
                     LAKLDVDVLFAVHRRLITAREWTHVERVIAEAVRRGDSARAGLADDLRDELVVHLLEC
                     GELAR"
     gene            complement(1028..2239)
                     /locus_tag="VFV99_21535"
     CDS             complement(1028..2239)
                     /locus_tag="VFV99_21535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5061972.1"
                     /translation="MENQRRARGPSMGDQFAPEPTDPKQALAQTAASGPAPAPGVPIV
                     PNAELSAGMRVGEYEVEAKIGEGAMGAVYRAVHPAIGRHVAIKVMTPKVFDEPESVKR
                     FVAEARAVAAIRHPGIVDVFGFGRLPDNRTYLVMEWLAGTSLSERMKHGTLEFEEACD
                     MLRQIARALEAAHGKGVIHRDLKPENVFVHLIDDEKPQCKLLDFGLAKVTNKEDGLVA
                     KTRTGQLLGTPLYMSPEQCKSKGVDHRTDIYALGCMAYEMLCGRVPFDCDNVAELISA
                     HLVQEPPKPRSLNPNLPDAIEKLLLQMVAKKPDERPTLADVRRTLSSVMSRQSQPIIA
                     AQVPDHPPPPPSFSSGSSPAIVMPPEVRESTATAEPAPVPRRMPGGQLPWIVGSLAII
                     AILLAALLALR"
     gene            complement(2369..3331)
                     /locus_tag="VFV99_21540"
     CDS             complement(2369..3331)
                     /locus_tag="VFV99_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061973.1"
                     /translation="MSQKFQASVQHRGDVSYVKLGGVIDEDNELSDLVDKIPSGTAVI
                     DLGEIERINSCGVRDWVNWLSKLEANGTRSVLVECSPAIVAQINLVNNFTGSGVVKSF
                     YVPYFCPECDEEKVLLVEASDMGPPPHEPPTCRCDECDLVMDFDDMPDSYFAFLSNQR
                     KLAEPEKINGAMKELARGSDAPQGAKGKVKSRQSAPQLGAPGSKPSVPSLPSIQRTST
                     APATTHSSGPRLPTGKSGHPSSNPPLGHKSSGNIPSTSKPPSQPRLGGKPPSSPQIAA
                     NMSMRMQAGAPAVAAPGKSNALVFTLVGILVAAIVVLAYLVLTK"
     gene            complement(3463..>4575)
                     /locus_tag="VFV99_21545"
     CDS             complement(3463..>4575)
                     /locus_tag="VFV99_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061974.1"
                     /translation="RGLARILLSSGDRPAAIEAYRGILNVEPDGASDRVALAEIYAVD
                     DPQRAIGELRKVLDRDIHHAPAYRLLSSFYTRTNDIERANRVLTALDLLGFAEETDRQ
                     TMARLRATRQNAPLRRSLDEQNRERLMANTAPREPLGEVWTAFAEEITAFIAQPSLGE
                     NVIPAEGNEDRLLQIAIEVGQLYELEPEIFVGDKVPGLLAVTAFPRQIVVVDRSLVGD
                     KDLALRFMFGYAFEAIRGGYAALLQLGARQRRELGQILRGLIQEVEPTGIPAELMTNA
                     NLRQAKVLERHAGTRDLDTGSWIDNMLACAKRAGLVACDDFAAAIWMVARLSGELLAS
                     HDDTVALGAVLGGPDLVRFYLSDNYQLIRDLLALPA"
BASE COUNT          730 a         1498 c         1523 g          824 t
ORIGIN      
        1 cgcgcgcaag ctcgccacac tcgagcaggt gcacgacaag ctcgtcgcgg aggtcatcgg
       61 cgagccccgc gcgggctgaa tcgccgcgcc gcaccgcctc ggcgatgaca cgctcgacgt
      121 gcgtccactc gcgcgcggtg atcaagcgac gatggaccgc gaacaacacg tccacgtcga
      181 gcttcgctag cccgacccgt acgaggtcgc gcagcgccag gtcggctgcg tgcgtggcgg
      241 tgagcttgtc gccggcaaga aactcgtccc acgatagacc agccactggc gatgcggacg
      301 cggtgcgcgc ctcgtccttg tcggcgtggc acttcttgta cttgagcccg ctgccgcacg
      361 gacacgggtc gttgcggccc acctgcttcg ccgcgcgtac cgtgaagccc gtcgcgcgtg
      421 tcgtctcgat ctcggcggga agcgatgcga tcacggcctc gagcgatgcg ttcatcgctt
      481 tctcgtcggc gctaacctgc ttcgcgtact ccttcgcgaa gggggcgatc ggcttcgtcg
      541 ccgcggcgac attcacatcg tcgatcgacg ctgcgatcgt cgcgagcagc gcatagctct
      601 cgacgctcat cggccgcgcg gacaaccggc ggagctccgg aatgacccgc tcggtcggcg
      661 cttcggcccg ccacgctgcg aacaccagga tcgcctcgag ctcgcatgca tccttcgtgt
      721 gtgggaaccg ccggttttcg acgagctcga ggagcttcgg ccggtcgtcc ttcgcgacga
      781 tcgcgacgag cgcgagcgtg atcgctggca actcgatccc gggtagcacg cgagcgacga
      841 gcggagccgg gacgtgttcg cctgcgcgcg ctcggccgag caccatcgtc gcggcggtct
      901 cgtagtcgca ggcctcgcat gcctcggtga tcgcgtcgat cgtcgcgctg cgtgccgcct
      961 cgacgtgggc gcgcgagatg cactcgccga cgagggctcg cgtgcgctct tctgccatgc
     1021 gcggacgtta gcgaagtgcg agcagcgccg cgagcaggat tgcgatgatc gcgagcgaac
     1081 cgacaatcca cggcagctga ccaccaggca tgcgtcgcgg cactggcgca ggttcagccg
     1141 tcgcggtgga ttcccgcacc tcgggcggca tgacgatcgc cggtgacgag ccgctgctga
     1201 atgacggcgg cggcggcggg tggtcaggca cctgggcggc gatgatcggc tgcgactgcc
     1261 ggctcatcac gctcgagagt gtgcgccgca cgtcggcgag tgttggccgc tcgtcgggtt
     1321 tcttcgcgac catctgcagc agtagcttct cgatcgcgtc cggtaggttc gggttcagcg
     1381 atcgcggttt cggcggttcc tgcacgaggt gcgcggagat caactcggcc acgttgtcgc
     1441 agtcgaacgg cacccgcccg cacagcatct cgtacgccat acagccgagc gcgtagatgt
     1501 cggtccggtg atcgacgccc tttgacttgc actgctcggg gctcatgtag agcggcgtgc
     1561 cgagtagctg cccggtgcgg gtcttcgcga cgagaccgtc ctctttgttc gtcaccttcg
     1621 cgaggccgaa gtcgagcagc ttgcactgcg gcttctcgtc gtcgatgaga tggacgaaca
     1681 cgttctcggg cttgaggtcg cgatgaatga cgcccttgcc gtgtgcggct tcgagcgcgc
     1741 gtgcgatctg gcgcagcatg tcgcacgcct cttcgaactc gagcgtgccg tgcttcatcc
     1801 gctcggagag gctcgtccca gcgagccatt ccatgacgag gtacgtgcgg ttgtcgggga
     1861 ggcggccgaa gccgaacacg tcgacgatgc caggatgccg gatcgcggcg accgcacgcg
     1921 cctcggcaac gaaccgcttc acgctctccg gctcgtcgaa caccttcggc gtcatcacct
     1981 tgatcgcgac gtggcggccg atcgcgggat gcaccgcgcg gtagacggcg cccatcgcac
     2041 cctcgccgat ctttgcttcg acctcgtact cgccaacgcg catgccggca ctcagctcgg
     2101 cattcggcac gatcgggaca cccggcgcgg gcgcggggcc tgatgcggcg gtttgtgcga
     2161 gcgcctgctt cgggtcggtc ggttcgggag cgaactggtc acccatcgac gggccgcgcg
     2221 cgcgtcgttg gttctccatg atcgtttcgt catggagcca ggcgccgtcg tccgggcaga
     2281 tcgattggtc ctgcggccaa gtcttgccgc agacctgaca aacgaccccc gcctttccct
     2341 ttagtgaatc ccccagagcc tgagaattct acttggtcag gacgaggtag gcgagcacca
     2401 cgatggccgc aaccagaatg cctacgaggg tgaacaccag tgcgttgctc ttgccgggcg
     2461 ctgccacggc gggagcaccg gcctgcatcc gcatcgacat gttcgcggcg atctgcgggc
     2521 tcgacggcgg cttgccaccg aggcgaggtt ggctcggcgg cttgctcgtc gatgggatgt
     2581 tgccgctgct cttgtgaccc agcggcgggt tgctcgacgg atggcccgac ttgccggtcg
     2641 gcaagcgcgg cccgctcgag tgcgtggtgg ccggcgccgt gctggtgcgc tggatcgacg
     2701 gcagcgacgg cacgctcggc ttgctgccgg gagcaccgag ctgcggcgcg ctctggcgcg
     2761 acttcacttt gcccttcgcg ccctgcggcg catcggagcc acgcgcgagc tctttcatcg
     2821 cgccattgat cttctcgggc tctgccaact tgcgctggtt cgagaggaac gcgaagtacg
     2881 agtccggcat gtcgtcgaag tccatgacga gatcgcactc gtcacagcga caggttggcg
     2941 gctcgtgcgg cggcggaccc atgtcgctcg cctcgacgag aagaaccttc tcctcgtcgc
     3001 actccgggca gaagtacgga acgtagaagc tcttgacgac gccgctgccg gtgaagttgt
     3061 tgaccaggtt gatctgggca acgattgccg gagaacactc gacgagaacc gatcgcgttc
     3121 cgttcgcttc gagcttgctg agccagttca cccagtcgcg aacgccacac gagttgatgc
     3181 gctcgatctc gccgagatcg atcaccgccg tgcccgacgg aatcttgtcg acgagatcag
     3241 aaagctcgtt gtcctcgtcg atgacaccgc cgagcttcac gtagctcacg tcgccgcggt
     3301 gttgaaccga cgcctgaaac ttttggctca tagttgcttc acgtaacggc cgtattgccg
     3361 attacaccac acccgttcaa ccctagaata gcgtgctggt cgtcgacgat ctagggaggc
     3421 taggtggttt tggtgcgcac accttctgtc tatcctacct cgtcaggcag gtagagcgag
     3481 caagtctctg atcaattggt agttgtcact taaataaaag cgcaccaaat caggcccgcc
     3541 gaggacggcc ccgagtgcga cggtgtcgtc gtggctggct agtaactcac ctgagagccg
     3601 ggcaaccatc cagatcgcgg cggcaaaatc gtcgcaggcg accagacccg cgcgttttgc
     3661 gcaggccagc atgttgtcga tccagctgcc ggtatcgaga tcacgcgtac cggcatggcg
     3721 ctcgagcacc ttggcctggc gcagattcgc gttcgtcatc agctcggccg ggatgccagt
     3781 cggttccacc tcctggatca ggccgcggag gatctggccg agctcgcgac gctggcgtgc
     3841 gccgagctgg agcagcgccg catagccgcc gcggatcgct tcgaacgcgt agccaaacat
     3901 gaatcgaagt gcgaggtcct tgtcaccgac gagcgaacgg tcgacgacga cgatctggcg
     3961 cgggaacgcg gtgacggcga ggaggccggg gaccttgtcg ccgacaaaga tctcgggctc
     4021 gagctcgtac agctggccga cctcgatcgc gatctggagc aaccgatcct cgttaccctc
     4081 ggcggggatc acgttctcgc cgagtgacgg ctgcgcgatg aacgccgtga tctcctcggc
     4141 aaacgcggtc cacacctcgc cgagcggctc gcgcggcgcc gtattcgcca tcaggcgctc
     4201 gcggttctgc tcgtcgagcg agcgacgcaa tggcgcattc tggcgcgtcg cgcgcaggcg
     4261 agccatcgtt tgccgatcgg tctcctcggc gaagccgagc aggtcgagcg cggtgagcac
     4321 gcggttcgcg cgctcgatgt cgttcgtgcg ggtatagaac gacgacagca agcgatacgc
     4381 gggcgcgtgg tggatgtcgc ggtcgagcac cttgcgcagc tcaccgatcg cgcgctgcgg
     4441 atcgtcgacg gcgtagatct cggcgagcgc gacgcgatcg ctggcgccgt cgggctcgac
     4501 atttaagatg ccgcgatacg cctcgatcgc ggccggccga tcgcccgacg agagcagaat
     4561 gcgcgcgaga ccgcg
//
LOCUS       DASUFX010000539         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_920201,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000539 DASUFX010000000
VERSION     DASUFX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920201"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFV99_21550"
     CDS             <1..222
                     /locus_tag="VFV99_21550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5061975.1"
                     /translation="GPGIPVTVRPRIFEPFFTTKSPDRGTGLGLSLSRGLVEAHHGTL
                     ELDDSSPRTRFVMRLPLTQPTQPRAVGEA"
     gene            complement(195..554)
                     /locus_tag="VFV99_21555"
     CDS             complement(195..554)
                     /locus_tag="VFV99_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5061976.1"
                     /translation="MILLVEDDEDVRDMLEFTLKSSGHVLELANHGRAALDALERQRP
                     CLIILDLLMPVMTGWQVLVEMKQRKLDDIPVCVISALGERIPSEAIASLRKPFDAKEL
                     LAIAARYWSHASPTARG"
     gene            651..1220
                     /locus_tag="VFV99_21560"
     CDS             651..1220
                     /locus_tag="VFV99_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061977.1"
                     /translation="MLWPPPYIALLVVLLAIAIVWWLSRRMDVAHPPVALMVTRIGFP
                     ANMRLADSSSALSMLELRDEIVIPFPKATLVIDFPLTHPAQIAIAAALPQGFTRGELV
                     QAICDEYAHVYAAEEGTASDDVVVEHRGTRRERVRSDGAYGIWGHALEDLVLRSARWV
                     KQSNGDVRIELHVDAKPIAPPPAAPELPS"
     gene            1220..1882
                     /locus_tag="VFV99_21565"
     CDS             1220..1882
                     /locus_tag="VFV99_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061978.1"
                     /translation="MWARLHKIDRIRPQKDGGALVLVEDERSAAMMEQRPPLSTLFAI
                     ARVLNAHRLLDAKYGGKGEVRYATNAKLPTFLFDAVRRAGASIGDRSGDKADVPAETA
                     SVSSVIDQAFTELGHEVRKTIGAHDMITALRKVEDRRRKEPLDRETQTVAYWTSVFEL
                     AALAGELSRPRGGRWIDTREIPVPYAIKFPEGGLATPTKLAQQIVEGGQPIESMATDF
                     TT"
     gene            1925..>3502
                     /locus_tag="VFV99_21570"
     CDS             1925..>3502
                     /locus_tag="VFV99_21570"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061979.1"
                     /translation="MKRALLAVVVLGCSSPSKPPPAPPSPPMTESKPAVAPTPPPTTE
                     QPPEDPYLWLEDVTGDKPIAWAKEQAAKSKAELEAVPGFTATRDRIRAILDSKEKTPY
                     VTKRGTYYYNFWRDAQNPKGLIRRTTLAEYKKKSPKWEVMLDIDALSKTENENWVYAG
                     NDCLYPTFDRCLVTMSRGGGDTAVVREFDIKTKKFLTDGFNLPAAKSEVAWKDRDTIY
                     VATDFGRGSLTKSGYPRIVKEWKRGTKLEDAVTVFEGQDSDISVNAFRSWDHGKSMDF
                     VNRGISFFAQELYVRGADGKLTKLDIPNDANGGIWNDQLFVTLRTEWKIGDKTWPAGA
                     LLTTPYADFQAGKRDFQMLFEPAPNKSLEGVTEMKTAVVVNELEDVKNKLYVWTFTKN
                     KWTKKPFGKDSLGTTSVSSVDGYSETDDYWLIHTDYLTPSTLSLGSLGKQAAKLKTSP
                     TFFDAKGLQVEQHFATSKDGTKVPYFQISRKAIALDGSNPTILYGYGGFEISLTPGYD
                     PISGSAWLERGGVYAIAN"
BASE COUNT          674 a         1157 c         1110 g          561 t
ORIGIN      
        1 ggtcctggca ttccggtcac ggtgcgcccg cggatcttcg agccgttctt cacgacgaag
       61 tcgcccgatc gcggcaccgg gctcggcctc agcctgtcgc gcggcctggt cgaggcacat
      121 cacggcacgc tcgagctcga cgacagctcg ccacggacgc gctttgtcat gcgactcccg
      181 ttgactcagc cgactcagcc acgcgcggtc ggcgaagcgt gagaccagta tcgcgctgcg
      241 atcgcgagca gctctttcgc gtcgaacggc ttgcgcagcg acgcgattgc ctccgacgga
      301 atgcgctcgc cgagcgccga gatcacgcag accggaatgt cgtcgagctt gcgctgcttc
      361 atctcgacga gcacctgcca cccggtcatc accggcatca gtagatcgag aatgatcagg
      421 cacggacgct ggcgctccag tgcgtcgagc gcggcgcggc cgtgatttgc aagctcgagt
      481 acgtgtccgc tgctcttgag cgtgaactcg agcatgtccc gcacgtcctc gtcgtcctcg
      541 accagcagga tcacagctac ctccatggta gcccgaagcg gccgtctaca gacacgcaga
      601 ggttgcgcct tcccgatgcg cgggcggcta atcattgcta gggtccggtc gtgctctggc
      661 cgccgccgta catcgcgctg ctcgtcgtgc tgctcgccat tgccatcgtg tggtggctga
      721 gccgtcgcat ggacgtcgcg catccgccgg tcgcgctgat ggtgacgcgg atcggctttc
      781 ccgcgaacat gcgcctagcc gactcgagca gcgcgctatc gatgctcgag ctgcgcgacg
      841 agatcgtgat cccgtttccg aaggcgacgc tcgttatcga cttcccgctc acgcatcccg
      901 cacagatcgc gatcgcggcc gcgctgccgc aaggcttcac gcgcggcgag ctcgtccagg
      961 cgatctgcga cgagtacgcg cacgtctacg ccgccgaaga aggcactgca tccgacgacg
     1021 tggtggtcga acaccgcggc acgcggcgcg aacgcgtgcg cagtgatggc gcctacggga
     1081 tctggggtca cgcgctcgaa gatctcgtgc tgcgctcggc gcgctgggtg aagcagtcga
     1141 acggtgacgt acggatcgag ctccacgtcg acgcgaagcc gatcgcgcca ccgccggcag
     1201 ccccggagct gccgagctga tgtgggcacg cctgcacaag atcgatcgca tccgtccgca
     1261 gaaagatggc ggcgcactcg tgctcgtcga ggacgaacgt agtgcagcaa tgatggagca
     1321 gcggcctccg ctctcgacgc tgttcgcgat cgctcgcgtg ctgaacgcac accgcttgct
     1381 cgacgcgaag tacggcggca agggcgaggt gcgctatgcg acgaacgcga agctgccgac
     1441 gtttctgttc gatgcggttc gccgtgcggg cgcgagcatc ggcgatcgca gcggcgacaa
     1501 agccgacgtt cccgccgaga cggcgtcggt gtcgtcggtg atcgatcaag cgttcaccga
     1561 gctcgggcac gaagtgcgca agacgatcgg cgcacacgac atgatcaccg cgctgcgcaa
     1621 ggtcgaagat cggcgccgca aagaaccgct cgatcgcgag acccagaccg tcgcctactg
     1681 gacatcggtg ttcgagctgg ccgcgctcgc cggggagcta tcgcggccgc gcggcgggcg
     1741 ttggatcgat acccgcgaga ttcccgtgcc gtatgcgatc aagtttcccg agggtggcct
     1801 cgccacacct acgaagcttg ctcagcaaat cgtcgagggg ggccagccga tcgagtcgat
     1861 ggccaccgat ttcacgacgt aaccgcgctc gacggcacgc aggatcgacg ttacagatcc
     1921 cggcatgaaa agagcgctgc tcgccgtggt tgtcctcgga tgttcatcgc cgtcgaagcc
     1981 cccgcccgca ccgccttcgc caccgatgac agaatccaaa cccgccgtcg ccccgactcc
     2041 accgcccacg acagaacagc cgcccgaaga tccgtacctc tggctcgagg acgtgaccgg
     2101 cgacaagccg atcgcgtggg cgaaagagca agccgcgaag agcaaggccg agctcgaagc
     2161 agtgccaggc ttcaccgcga cgcgtgatcg cattcgcgca attctcgatt cgaaagagaa
     2221 gacgccgtac gtcacgaagc gcggcaccta ctactacaac ttctggcgcg acgctcagaa
     2281 cccgaagggg ctcatccggc gcaccacgct cgccgagtac aagaagaagt ccccgaagtg
     2341 ggaagtcatg ctcgacatcg acgcgctgtc gaagacggag aacgagaact gggtctacgc
     2401 cggtaacgac tgcctgtatc cgacgttcga tcgctgcctg gtcacgatgt cgcgcggcgg
     2461 cggcgacact gccgtcgtcc gcgagtttga catcaagacg aagaagttcc tcaccgacgg
     2521 cttcaacctg ccggccgcga aaagcgaagt cgcctggaaa gatcgcgaca cgatctacgt
     2581 cgcgaccgac ttcggtcggg gctcgctgac gaagtccggc tatccgcgca tcgtcaagga
     2641 gtggaagcgc ggcacgaagc tcgaggacgc ggtcaccgtg ttcgagggcc aggacagcga
     2701 catctcggtc aacgcgttcc ggtcgtggga ccacggcaag tcgatggact tcgtgaaccg
     2761 cggtatctcg ttcttcgcgc aggagctcta cgtccgcggc gcggacggca agctcacgaa
     2821 gctcgacatc ccgaacgatg cgaacggcgg catctggaac gatcagctgt tcgtcacgct
     2881 gcgcaccgag tggaagatcg gcgacaagac ctggcccgcc ggcgcgctgc tgacgacgcc
     2941 gtacgccgac tttcaggccg gcaagcgcga cttccagatg ctgttcgagc ccgcgccgaa
     3001 caagtcactc gaaggcgtca ccgagatgaa gaccgcagtc gtcgtcaacg agctcgagga
     3061 cgtcaagaac aagctctacg tgtggacgtt cacgaagaac aagtggacga agaagccgtt
     3121 cggcaaggac tcgctcggta cgacgtcggt cagctcggtc gacggctaca gcgagacgga
     3181 cgactactgg ctgatccaca ccgactacct cacgccatcg acgctgtcgc tcggctcgct
     3241 cggtaagcaa gcggcgaaac taaagacgag cccgacgttc ttcgatgcga aaggcctgca
     3301 ggtcgagcag cacttcgcga cgtcgaagga tggcaccaag gttccgtact tccagatctc
     3361 gcgtaaggcg atcgcgctcg acggcagcaa cccgaccatc ctctacggct acggcggctt
     3421 cgagatctcg ctcacgccgg gctacgatcc gatcagcggc tccgcgtggc tcgagcgcgg
     3481 cggtgtgtac gcgatcgcga ac
//
LOCUS       DASUFX010000540         8701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_85903,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000540 DASUFX010000000
VERSION     DASUFX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85903"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="pncA"
                     /locus_tag="VFV99_21575"
     CDS             <1..615
                     /gene="pncA"
                     /locus_tag="VFV99_21575"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF008623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX5061980.1"
                     /translation="MRALLLIDLQYDFCPGGALAVANGHETIPVANRLMPKFSTIVAT
                     QDWHPAHHGSFAVNHPGKKPYDMHELAGLPQVMWPAHCVQNTPGAELHRDLDRNLITE
                     VFQKGTDPAIDSYSGFYDNGHRKATGLGDWLKQRDIKHVYVMGLATDYCVKFTTLDAR
                     QLGFDVSLVVDGCRGVNLNPDDSAKAIEEMRAAGVVIVESKDVE"
     gene            707..1228
                     /locus_tag="VFV99_21580"
     CDS             707..1228
                     /locus_tag="VFV99_21580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5061981.1"
                     /translation="MDELATLVGRARVGDRRAFAELYRRFHRAVHGVVLAKVSYSDAA
                     DLVQDVFAIALERLPQLADPAAFAGWLMSIARNRAIDHVRGKKPTDELADIPVPPPRS
                     AEVAEVLAALRALPEAYQETMILRLVEGMSGPEIAEQTGLSPGSVRVNLHRGMKLLRE
                     RLGIASQEASDVG"
     gene            1218..2246
                     /locus_tag="VFV99_21585"
     CDS             1218..2246
                     /locus_tag="VFV99_21585"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5061982.1"
                     /translation="MSDDYLFDRSGPPDPDVAKLEKLLAPLRHDAPLDELRMRRKRRT
                     PWFVLGVALAAAAALVIYFALPAKPTKPSACGGSAGFAFSGVGGDVSCGGAQVAHGVL
                     PVGGQLDTGPHEASLTIADIGSAELGKQTRVRLERTDRERHQLSLERGHMHAKVAAPP
                     RLFAVSTKHTEVVDLGCEYTIDVDDTGAGSICVATGLVELATASRATVVVPEGACATI
                     LAGQRPGLPHARDARPELVSAIDAYDRGDPAAFDRLLTLAERRDAITLIALAAIEGDP
                     NPSPKGCVVGRCRSVLERLMELSPPPDAEITVDSALTDPNHFGAWRNDILEIYYGMWG
                     PRAGKKQP"
     gene            2263..2625
                     /locus_tag="VFV99_21590"
     CDS             2263..2625
                     /locus_tag="VFV99_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061983.1"
                     /translation="MRARTHLTPGTSAAGCWRMSQDKDPNQGEGDRISGRRYDRHVRE
                     FIAEGKVPDAANDARLYVERDPDEAAKAERFARGGPSSSRSASVEEIVEKGQSVFDRV
                     RPMVKRAVGNLRARFGRK"
     gene            2661..2918
                     /locus_tag="VFV99_21595"
     CDS             2661..2918
                     /locus_tag="VFV99_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5061984.1"
                     /translation="MHILTTLIVGLIVGALAKLIMPGKDPGGFIITILLGIAGAFIGG
                     WIGHALGWYKAGEGPGIIVSVIGAIILLGIYRLVIGRRRVV"
     gene            2929..3255
                     /locus_tag="VFV99_21600"
     CDS             2929..3255
                     /locus_tag="VFV99_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061985.1"
                     /translation="MAEHQHSLHPIIRPPEEFEHVNSPVRDDEALGPTEDLDREPDGV
                     PHQVVGLTAFFSVMLAMLIAFMALFGGTVSRVAAIILAVLAIPVLVSTLRGKAARERS
                     TLHPSR"
     gene            3282..5141
                     /locus_tag="VFV99_21605"
     CDS             3282..5141
                     /locus_tag="VFV99_21605"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5061986.1"
                     /translation="MAHGIAILLLAIGCGKKVVHHPGEEFLDAIVFEGNTSIKSSDLR
                     AGLALRRVQKRGASPDPYLVVVDGQRILGEYLRRGFLEADVRSRVERHGDRTTVIYTI
                     HEGPRAKTRVMITGLPPDDPDLFKKVRNTLPLEDGEYFLYAPYDEAKEKMLGVVEDAG
                     FAHAQLNAHVIADRANHLAIVHLQYDIGPKCKFGTIDISGVGGELADAVRERVAFDSG
                     EQYSSSAIAQTQRNLYEMRRFSTVRVLPDKSDGDVINVRISLAHATRNEIELGGGLGM
                     DPATYEVRARTGYSKLGWPFPLTDLTIDLRPAYAMLRDGSGYEPRIRALTSLRRMDLF
                     RPFVTGTAEVGYNYVTVEAYTNYGPVVRLGAETPLFAMNELRLRAGWELDYNNFRHIS
                     PLIDPALEVALGLDHPERVGMYTQTLSLDLRDNPLEPRLGAFAEVRIDEGTKWAGGAF
                     EYVRVTPEVRGFVPVPTMPVVLAARVRAGRFYGEVPATQRYFSGGATTQRGFAERHLA
                     PTVVGIVDGDMEDVPVGGAELFESNFEIRSRLTKVRGIPIGLVAFLDGGDCTEVGMLD
                     LGNLNWAIGPGLRAFTKVGAIRLDVGYRLNRTGPGNPEPDSHYAFHLSIGEAY"
     gene            5141..>8701
                     /locus_tag="VFV99_21610"
     CDS             5141..>8701
                     /locus_tag="VFV99_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061987.1"
                     /translation="MRKVHGWRRVARWVIGIVGGFVGLVALVVIVAFIVFSTGWGRGI
                     LKNQIATRLDKAFVGGASIGRVDGNPLSELVLHDIVINGPDKQPAITVKQLTVRLPLL
                     PLISHQLRVEKVIAEDLEVRAKRLRNGDYNVDNLMNKSEPSTWSVYLPNLEVRRGHVL
                     VEQGQGAEPIDIDNIEVNVDAALPFAGPMSANARVTGQWRQKRAPISIGAVIHGDAET
                     FEVRNAGVQVGDVRAIALGVKLPKGTFAKPYAGTVAVSAPAKAVRELVPSVRLPEDIA
                     LAVTVRPGGRRTDFEIAGAIGEGQVSAFGSADMQAKLASVVVAATDLNLTKLTNGNVV
                     GHGGAFAALELDGAAAGEVPTVTGMVHAWPVLDDTPPLEIIAGIDSAGDRIRATVGAV
                     SDSGIRAGIDADVLKRGETITLERADLIASTLDVGRATAGKAPLRGVLTANLHAEGEL
                     APRMDLALAGHANGRRIRMAGASAQRLAMRIDAKHLPSHPAGSGRVELLDVRRGDLQF
                     SNLTVAAGNRPDGKLQVSVRSHPKPAPWRVDLDALVTTGDTIVVDLQRHFVRAAGGST
                     WTGNTGRVTIGPRKIEVRNFKSSGGNGTVTADASYVRAGRDQGDLAARVDASFDLGNL
                     MQAHKGHVEAHVDAKRADGRFTGTVVATAKGVTLDPKSPVAFDGDVKIEARGVQRQVL
                     AHASVSTAKAGSLAFALDVDAPTDITNARAWKRLDRNAIRTAKLTVKQLDLAMISKVA
                     GSRAPALSGNVNGSLELSPARAGGTIAIRGVQTQQTKDLGSITADLKVAQDGAGELKT
                     TLTARLEPNPNAVAAKDITKNGQAHLLLDATFRTPDRIFDPAAWKRLGPNAFGGATLR
                     AERLAFEPGTLERLGIVSSMRGELAVGADVDPGMKSVRFAINLYQLRGGLFAQPIAVS
                     VVGAVDEQSTRANADIRGQGVTLLHASTEIPVTLDQLRRDAQSAKSAPLSGQVRIDHV
                     PAKALMNVIGTSQITGGTLDGTIDLAGTVADPTVDAKLVAQNVTVPNEGTREVQQIKQ
                     LTIAAKWDGTAGNVAIDSDQTGGGKLTVRAAGAPSDLDKVSATIKATKLDIAPLVAFM
                     PGPAGGLAGRLDADFALRGANPRTADLAGSLHVTDGRIPIAPTVGTLFRGDVKLDVKN
                     KQVGLNLTGKLGRGDVALTANAPLDGISPTNGELKLTVRNV"
BASE COUNT         1529 a         2801 c         2913 g         1458 t
ORIGIN      
        1 atgcgagcgc tgctcctcat cgacctgcag tacgacttct gcccgggtgg tgcgctcgcc
       61 gtcgcgaacg gccatgaaac gattccggtc gcgaaccggc tcatgccgaa gttctcgacg
      121 atcgtcgcga cgcaagactg gcatcccgcg catcacggca gcttcgcggt gaatcatccc
      181 ggcaagaagc cgtacgacat gcacgagctc gctggcttgc cgcaggtgat gtggcccgct
      241 cactgcgtgc aaaacacgcc gggcgccgag ctgcatcgcg atctggatcg caacttgatc
      301 accgaggtgt tccagaaggg caccgatccg gcgatcgaca gctactcggg cttctacgac
      361 aacggtcatc gcaaggcgac cggtcttggc gactggctga agcagcgcga catcaagcac
      421 gtgtacgtga tgggcctcgc gaccgactac tgcgtgaagt tcacgacgct cgacgcgcgt
      481 cagctcggct tcgatgtgtc gctcgtggtc gacggctgcc gcggcgtcaa cctgaacccc
      541 gacgacagcg cgaaggcgat cgaggagatg cgcgccgccg gcgtggtcat cgtcgagagc
      601 aaagacgtcg agtaatctaa agctcttccg cgcccacgag gccacattgt taacagcccg
      661 agtcctgccc cgtcttgcct gggtgcggct cgcgtacagt atcggcgtgg atgagcttgc
      721 gacgctggtc ggccgcgcac gcgtgggcga tcgcagggcg tttgccgagc tgtaccggcg
      781 cttccaccgc gcggtccacg gtgtcgtact cgcgaaggtc tcgtacagtg acgccgcgga
      841 tctcgtgcag gacgtgtttg cgatcgcgct cgaacgactg ccgcagctcg ccgaccctgc
      901 cgcatttgcc ggctggctca tgtcgatcgc tcgcaatcgc gcgatcgatc acgtgcgcgg
      961 caagaagccg accgacgagc tcgccgatat tccggtgccg cctccgcgca gcgccgaggt
     1021 ggccgaggtg ctcgcggcgt tgcgcgcgct acccgaggct tatcaggaga caatgatctt
     1081 gcgcctcgtc gaaggcatga gtggaccaga gatcgccgag caaaccgggc tgtcaccggg
     1141 gtccgtgcgc gtcaaccttc atcgaggcat gaagctcctg cgcgagcgcc tcggaattgc
     1201 tagtcaggag gcatccgatg tcggatgact atctgttcga tcgatccggc ccaccggatc
     1261 ctgatgtcgc gaagctggag aagctgctcg cgccactacg tcacgatgcg cccctcgacg
     1321 agcttcgcat gcgtcgcaag cgccgcacgc cgtggttcgt gctcggtgtt gcgctcgctg
     1381 cggcagccgc gctcgtcatc tatttcgcgt tgccggcgaa gcccaccaag ccatctgcgt
     1441 gtggcggcag cgcagggttc gcattctcgg gcgtcggcgg cgatgtgtcg tgcggtggcg
     1501 cgcaggtcgc gcacggcgta ttgccggttg gcggtcagct cgacaccggc ccgcatgaag
     1561 cgtcgctcac gatcgcggac atcggcagcg ctgagctcgg taagcaaact cgcgttcgcc
     1621 tggagcggac ggaccgcgag cgtcatcaac tgtcgctcga gcgtggtcac atgcatgcga
     1681 aggtcgcggc gccgccgcgc ttgttcgcgg tgtcgacgaa gcacaccgag gtcgtcgatc
     1741 tcggctgcga gtacacgatc gatgtcgatg ataccggtgc cggctcgatc tgcgtggcga
     1801 ccgggctcgt cgagctcgcg acggcttcgc gcgcgactgt cgtcgtgccc gagggtgcgt
     1861 gcgcgacgat tctcgcaggc cagcggcctg ggttgccgca cgctcgcgac gcgcgtccgg
     1921 agctcgtctc tgcgatcgat gcttacgatc gcggcgaccc ggccgcgttc gatcggctgc
     1981 tcacgctcgc cgaacgccgc gacgccatca cgttgatcgc gcttgctgcg atcgagggcg
     2041 atcccaaccc cagcccgaag ggctgcgtgg tcggccgttg ccgctctgtg ctcgagcgcc
     2101 tcatggagct gtcaccaccg cccgatgccg agatcaccgt agattctgcg ctcaccgatc
     2161 cgaatcactt cggtgcttgg cgcaatgaca tcctcgagat ctattacggg atgtggggac
     2221 ctagagccgg caagaaacag ccgtagctcg taagcgcgca gcgtgcgtgc gcgcacgcac
     2281 cttacgcctg gcacaagtgc tgctggttgt tggcgcatga gccaggacaa ggacccgaat
     2341 cagggcgaag gcgacaggat ttcggggcgg cgctacgacc ggcatgtgcg cgagttcatc
     2401 gcggagggca aagtgcccga cgccgcgaac gatgcgcgtc tgtacgtcga gcgcgatccg
     2461 gacgaagccg cgaaagcaga gcgattcgct cgtggcggcc cctctagcag ccgatcggcc
     2521 tccgtcgaag agatcgtcga aaaaggtcaa tcggtgttcg atcgcgtgag gccgatggtc
     2581 aaacgcgcgg tcggcaacct gcgtgcacgt tttgggcgga agtagggcgt gattaaccac
     2641 tcaaccttgg agcatctctt atgcacattt tgactacttt gattgttggt ctcatcgtcg
     2701 gtgcactcgc gaagctgatc atgccgggca aggatccggg cggcttcatc atcaccatcc
     2761 tgctcggcat cgccggcgcc ttcatcggcg gctggatcgg gcacgcgctc ggttggtaca
     2821 aggccggcga gggcccagga atcatcgtgt ccgtgatcgg cgcgatcatc ctcctcggga
     2881 tctatcgcct cgtgatcggt cgccgacgcg tcgtgtgaag gaggaaccat ggcggaacat
     2941 cagcattcac tgcatccgat cattcggccc cccgaggaat tcgagcacgt gaactcaccc
     3001 gttcgcgatg acgaagctct cgggccaacg gaggatctcg atcgcgagcc cgacggcgtg
     3061 cctcatcagg tcgtcggact cacggcgttc ttctccgtga tgctcgcaat gctgatcgcg
     3121 ttcatggctc tgttcggagg aacggtgagt cgtgtcgcgg cgatcatcct cgcggtactc
     3181 gcgattccgg tgctcgtctc gacactacgt ggcaaggcgg caagggagag gtcaacactt
     3241 catccatcga gataaccgtg gtagcggcgc acgccagggc gatggcgcac gggatcgcga
     3301 tccttctgct cgcgattggt tgcggcaaga aggtcgtcca tcatccagga gaggaattcc
     3361 tcgacgccat cgtcttcgag ggaaatacct cgatcaagtc gagcgacttg cgcgcggggc
     3421 tcgcgttgcg gcgcgtccag aagcgtggcg catcacccga cccgtacttg gtagtagtag
     3481 acggtcagcg gatcctcgga gagtacctgc gtcgcggttt cctcgaggcc gacgttcgct
     3541 cacgcgtcga gcgacacggc gataggacga cggtcatcta cacgatccat gagggtccgc
     3601 gcgcgaagac gcgcgtgatg atcacgggcc tgccgcccga tgatccggat ctgttcaaga
     3661 aggtccgcaa cacgttgccg ctcgaagacg gcgagtactt cttgtacgcg ccttacgacg
     3721 aggcgaagga gaagatgctc ggcgtcgtcg aggatgccgg gttcgcgcac gcgcagctca
     3781 atgcacacgt gatcgccgat cgcgcgaacc atctggcgat cgttcacctc cagtacgaca
     3841 tcgggccgaa gtgcaagttc ggcacgatcg acatctcggg tgtcggcggc gagctcgctg
     3901 acgccgtgcg tgagcgcgtc gcgttcgata gcggcgagca gtactcgtcg tcggcgatcg
     3961 cgcagacgca gagaaacctg tacgagatgc gccggttctc gaccgtgcgc gtcctgcccg
     4021 ataagagcga tggcgacgtg atcaacgtcc gcatctcact cgcgcacgcg acgcgcaacg
     4081 agatcgagct gggtggcggc ctcggcatgg accccgcgac atacgaggtg cgcgcgcgta
     4141 ccggttactc gaagctcggc tggccgttcc cgcttaccga cctgacgatc gatctgcggc
     4201 ccgcctacgc gatgctgcgc gacggcagcg gctacgagcc acgcattcgc gcgctgacgt
     4261 cgctgcgtcg catggatttg ttccggccct tcgtcaccgg cacggcggag gttggctaca
     4321 actacgtcac cgtcgaggcg tacacgaact acggaccggt cgtccgactc ggggcagaga
     4381 cgccgctgtt cgcgatgaac gagctgcgac tgcgtgccgg ctgggagctc gactacaaca
     4441 atttccgcca catctcaccg ctgatcgacc ccgcattaga ggtggcactc ggtctcgacc
     4501 atcccgagcg agtcggcatg tacacgcaga cgttgtcgct cgacctgcgc gacaacccgc
     4561 tcgagccgcg cctcggagcg tttgcagagg tccgcatcga cgagggcacg aagtgggccg
     4621 gcggcgcgtt cgagtacgtc cgtgtcacgc cggaggtgcg cggcttcgtg ccggtgccga
     4681 cgatgccagt cgtactcgcg gcgcgagtgc gcgccggtcg gttctacggt gaggttcccg
     4741 cgacccaacg ttacttctcc gggggtgcga cgactcagcg tgggttcgcc gaacggcatc
     4801 tcgcgccgac ggtcgtcggc atcgtcgacg gcgacatgga agacgtgccg gtcggtggcg
     4861 cggagctgtt cgaatccaac ttcgagatcc gttcgcggct aacgaaggtg agaggcatcc
     4921 cgatcggcct cgtcgcgttc ctcgatggtg gcgactgcac cgaggtcggc atgctcgatc
     4981 tcggaaacct gaactgggca atcggtcctg gcctcagggc gttcacgaaa gtcggcgcga
     5041 ttcgcttgga tgtcggctac cggttgaatc gcaccggtcc cggcaacccg gagcccgact
     5101 cgcactacgc attccacctc agcatcgggg aggcgtactg atgcggaagg ttcatggctg
     5161 gcgccgcgtc gcgcgctggg tcattggcat cgtcggcgga ttcgtcgggc tggtcgcgct
     5221 cgtcgtgatc gtcgcgttca tcgtgttctc gaccggctgg ggacgcggca tcttgaagaa
     5281 ccagatcgcg acgcgactcg acaaagcatt cgtcggcggc gcgtcgatcg gtcgtgtcga
     5341 cggaaacccg ctctccgagc tcgtgctcca cgacatcgtg atcaacggcc ccgacaagca
     5401 gccggcgatc acggtgaagc agctgaccgt taggttgccg ctgttgccgc tgatctcgca
     5461 tcagctgcgc gtcgagaagg tgatcgccga ggacctcgag gtgcgcgcga agaggctccg
     5521 caatggcgac tacaacgtcg acaacctcat gaacaagtcg gagccgtcga cgtggagcgt
     5581 gtatctgccg aacctcgagg tgcgccgcgg tcatgtgctc gtcgagcagg gccagggtgc
     5641 cgagccgatc gacatcgaca acatcgaggt gaacgtcgac gcggccctgc cgttcgcggg
     5701 gccgatgtcg gcgaacgcgc gcgtgaccgg gcagtggcgg cagaagcgcg cgccgatttc
     5761 gatcggagcg gtgatccatg gcgacgccga gacgttcgag gtgcgcaatg ccggcgtgca
     5821 ggtcggcgac gttcgcgcga tcgcactcgg cgtgaagttg ccgaagggca cattcgcaaa
     5881 gccgtacgcg ggcaccgtcg cggtcagcgc gccggcgaag gccgtacgtg agctcgtgcc
     5941 atccgtgcgt ttgcccgagg acatcgcgct cgcggtgacg gtgcgtcccg gcggacgtcg
     6001 gaccgacttc gagatcgcgg gcgcgatcgg cgagggccag gtatctgcat tcggcagcgc
     6061 cgacatgcag gcaaagctcg ccagcgtcgt cgtcgcggcg accgacttga acctcacgaa
     6121 gctgacgaac ggcaacgtgg tcggccacgg cggcgcattc gcagcgctcg agctcgacgg
     6181 cgcggcggcg ggcgaagtgc caacggtcac cggcatggtg cacgcgtggc cagtgctcga
     6241 tgacacgccg ccgctcgaga tcatcgctgg catcgacagt gccggtgatc ggattcgcgc
     6301 gacggtcggt gccgtgagcg acagcggcat tcgcgccggc atcgatgcgg acgtgctcaa
     6361 gcgcggcgag acgatcactc tcgagcgcgc tgatttgatc gcgagtacgc tcgacgtggg
     6421 ccgcgcgacc gcaggtaaag caccgctgcg cggcgtgctc acggcgaacc tgcatgcaga
     6481 aggcgagctc gcgccgcgca tggatctcgc gctcgccggc catgcgaatg gccgcaggat
     6541 ccggatggcc ggtgcgagtg cgcagcggct ggcgatgcgc attgatgcga agcatctgcc
     6601 gtcgcatccg gccggtagtg gccgcgtcga gctgctcgat gtgcggcgtg gcgacttgca
     6661 gttctcgaac ctcacggtcg ccgcgggcaa ccggcccgac ggcaagctgc aagtctccgt
     6721 acgctcgcac ccaaagcccg cgccgtggcg cgtcgatctc gacgcactgg tgacgacggg
     6781 cgatacgatc gtcgtcgacc tacaacgcca cttcgtgcgt gcggccggcg gctcgacgtg
     6841 gaccggcaac actggtcggg tcacgatcgg cccgcgcaag atcgaggtcc gcaacttcaa
     6901 gagcagcggc ggcaacggca cggtcaccgc ggacgcgagc tatgtgcgtg cgggtcgcga
     6961 ccagggcgat ctcgcggcac gcgtggacgc atcgttcgac ctcggcaatc tgatgcaggc
     7021 gcacaagggc catgtcgaag cgcatgtcga cgccaagcgc gccgacggtc gcttcaccgg
     7081 caccgtggtc gcgacggcaa agggcgtgac gctcgatccg aagtcgccgg tcgcgtttga
     7141 cggcgacgtg aagatcgaag cccgtggcgt ccaaagacag gtgctcgcgc atgccagtgt
     7201 atccactgcg aaggccggca gcctcgcgtt cgcgctcgac gtcgacgcgc cgacagatat
     7261 cacgaacgca cgggcctgga agagactcga tcgcaacgcg atccgcaccg cgaagctgac
     7321 ggtgaagcag ctcgatctcg cgatgatctc gaaggtcgcc ggctcgcgcg cacctgcgct
     7381 tagcggcaac gtgaatggct cgctcgagct gtcgcccgcg agggcaggcg gcacgatcgc
     7441 gatccgcggc gtgcagacgc aacagacgaa agacctcggc agcatcactg cggatctgaa
     7501 ggtcgcgcag gacggcgccg gcgagctgaa gaccacgctt accgcaaggc tcgaaccgaa
     7561 tccaaacgcg gtggccgcga aggacatcac gaagaacggg caggcacacc tgctcctcga
     7621 cgcgacgttc cgcacacccg atcggatctt cgatccggcc gcgtggaagc ggctcgggcc
     7681 caacgcattc ggcggcgcaa cactgcgcgc cgagcgcctc gcattcgagc ccggcacgct
     7741 cgagcgactc ggcatcgtct cgtcgatgcg cggcgagctc gcggtcggtg ccgatgttga
     7801 ccctgggatg aagtcggtgc gctttgcaat caacctctat cagctgcgcg gtggtctgtt
     7861 cgcacaaccg atcgcggtca gcgtcgtcgg tgcagtcgac gaacaatcga cgcgcgcgaa
     7921 cgcagacatt cgcggccaag gcgtcacgct cctgcatgca tcgacggaga ttccggtgac
     7981 gctcgatcag ctgcgccgtg acgcgcagag cgccaagtcg gcaccgctct ccggccaggt
     8041 gcgcatcgat cacgtaccgg cgaaggcgct gatgaacgtg atcggcacga gccagatcac
     8101 gggcggcacc ctcgacggga cgatcgatct cgcgggtacg gtcgccgatc cgaccgtcga
     8161 cgccaagctc gtggcgcaga acgtgacggt gccgaatgag ggcacgcgcg aggtccagca
     8221 gatcaagcag ctcacgatcg cggcgaagtg ggacggcact gcaggcaacg tcgcgatcga
     8281 cagcgatcag accggcggcg gcaagctgac cgtgcgcgcg gcaggtgcgc cgagcgacct
     8341 cgacaaggtc agcgcgacga tcaaggcgac gaagctcgac atcgcaccgc tcgtcgcgtt
     8401 catgccggga cccgccggcg ggctcgccgg gcgactcgac gctgacttcg cactgcgcgg
     8461 cgcgaatcca cgcaccgccg atctcgcggg ctcgctccac gtcaccgacg gccgcattcc
     8521 gatcgcaccg actgtcggta cgctgttccg aggcgacgtg aagctcgacg tgaagaacaa
     8581 gcaggtaggg ctcaacttga cgggcaagct tggccgcggc gacgtcgcgc tcactgcgaa
     8641 cgcaccgctc gacggcatct caccgacgaa cggcgagctg aagctgacgg tccgcaacgt
     8701 t
//
LOCUS       DASUFX010000541         4275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_85955,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000541 DASUFX010000000
VERSION     DASUFX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85955"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFV99_21615"
     CDS             <1..655
                     /locus_tag="VFV99_21615"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061988.1"
                     /translation="HASGQVASDIAIRTIVHSLKTGDPAPAPGVDPLTAAMKAANAAV
                     YQRSLSDPACHGMGTTAVGLRFEENVVHICHCGDSRAYLLRHGQLSPITRDHSLRNLY
                     QDRPDLVGTLGPATSNVIIRAVGLDATVEIEHNQMLPEHNDIYLLCCDGLSDLVDDWM
                     IREIMTSGDSLEVVAENLIRAANNNGGTDNITVVLAQTFVDPLWVPAHAYPQQMQRY"
     gene            complement(652..2226)
                     /locus_tag="VFV99_21620"
     CDS             complement(652..2226)
                     /locus_tag="VFV99_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061989.1"
                     /translation="MRVAFLFVTAACSVTGSGTDRPEPLAPLPPPAHVETERFRDAEA
                     CGQCHLVADDTPVLHDADGANVSPVLLWRSSLMANAARDPYYLAVVAEELARAPAKTA
                     DIEKLCTRCHGPAGSEELADSGSHVSFDELTAGTSPAATLARGGVTCTLCHQITSGSL
                     DGDSAFAGKFAVGYQRKIFGAYSNPMTQPMMLIVNYEPTYGAHISSSALCGSCHTVIL
                     PRPTGEVVEQATYLEWRASELPAKNQPCQYCHVPAVDDAGRAISTPIASFPASLAPRQ
                     PIGRHTFVGGNSYILTLLADAIEWSGVGIDASELLASAARDDAHLATAARLTVTDSHR
                     EGGSIVATVRVENLTGHKLPTGYPSRRVWLHVTARNAGNVVFESGNVATLTAQPHRDE
                     ITAPEQAQVWEATLVDAAGQPTHRALDARRYGKDDRILPKGFAPTSTDRARTAPVGVA
                     GDASFVAGSDDVMYRFAAPAGTTIEIELLYQALRPEIVDAIEASATPAGSRFVDLARA
                     RPVTPIVIARTTSAAL"
     gene            complement(2356..3327)
                     /locus_tag="VFV99_21625"
     CDS             complement(2356..3327)
                     /locus_tag="VFV99_21625"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061990.1"
                     /translation="MTVACERIARVLVIALVAAASAAQAQTPSPNQAAADEAFKAGRD
                     LYKAGKYAEACEQFEKSQKLDPANGTLFNLSQCSEKVGKLATAAAGYRELITKDTNDQ
                     RKATAAERLKEITPKIPKLIVKVDKPPPGIVVEIDSKTGPRGIPANTPIEVDVGEYSV
                     VARARGYNEFMSRVKIAPDGKTTAVEATLEAGASNTETVGIAPRQKGPDKPRNTKKLL
                     GIGGMATGGAALVGGIVLGSLARSKWNDAKAVCGGLTCTTQADVDRANDLGDQAHTKA
                     TWSTVFLVAGVAIGGAGTYLFLTAPAGTTITPTASDHSAGVTLSGQF"
     gene            complement(3311..>4275)
                     /locus_tag="VFV99_21630"
     CDS             complement(3311..>4275)
                     /locus_tag="VFV99_21630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5061991.1"
                     /translation="SNVVGSPGYMSPEQLKSSKTVDARSDIWSLGVVLYELVSGRKPF
                     LGESITELALRVAMDPTPPLGGGVPPLFESVILRCLEKEPHRRFKDVAELAQALAPFV
                     GPVRGAELAYAAARVLRGAYTPLPTSTAAPGTNTPTTLRGANAVLAPSGIRNKRNLRL
                     PIVIASGAVVGIVLALILVIGVKTKHPTRATTPAEASATTGTTKMQVAPSAAPAAAKQ
                     EPPVTNAAETKPVETKPAETKPAETKPAVTKAAATKSVETKAAETKTAKAAETKTAKA
                     AETKTAKAETKKSSSKKSSSPKSSKTKAEPKTTKTAGDIGDSRL"
BASE COUNT          625 a         1450 c         1419 g          781 t
ORIGIN      
        1 tcacgcgtcg ggccaggtcg catcggacat cgcgattcgc acgatcgtcc actcgctcaa
       61 gaccggtgac ccggcgccgg cgcccggcgt cgatccgctg actgcggcga tgaaggcggc
      121 aaacgcggcc gtgtatcaac gctcgctcag cgacccggcg tgtcacggca tgggcacgac
      181 ggccgtcggc ctgcgcttcg aagagaacgt cgtgcacatc tgccactgcg gcgattcgcg
      241 cgcgtacttg ctgcgccacg gtcagctgtc accgatcacg cgtgaccact cgctgcgcaa
      301 tctctatcag gaccgccccg acctcgtcgg cacgctcggt ccggcgacgt cgaacgtgat
      361 catccgcgcc gtcggtctcg atgccaccgt cgagatcgag cacaaccaga tgctgccgga
      421 gcacaacgac atctacttgc tgtgctgcga tggcctgtcg gatctggtcg atgactggat
      481 gatccgcgag atcatgacat cgggcgattc gctcgaggtc gttgccgaga acctgatccg
      541 tgccgcaaac aacaatggcg gcaccgacaa catcacggtc gtgctcgcgc aaacgttcgt
      601 cgatccgttg tgggtgcccg cgcacgcgta cccgcagcag atgcagcgct actaaagcgc
      661 ggccgacgtc gttcgtgcga tcacgatcgg cgtcaccggt cgtgcacgcg ccagatcgac
      721 gaaccgcgaa cccgcgggag tcgccgacgc ttcgatcgca tcgacaatct ccgggcggag
      781 cgcctggtag aggagctcga tctcgatcgt cgtaccggca ggcgccgcga agcgatacat
      841 cacgtcatcg gacccagcga cgaagctcgc atcgccggcg acgccgaccg gtgcggtgcg
      901 cgcgcgatcc gtgctcgtgg gtgcgaagcc cttcggcagg attcgatcgt ccttgccgta
      961 gcggcgcgcg tcgagtgcgc gatgtgtggg ctgccccgcc gcgtcgacga gcgtcgcctc
     1021 ccagacctgc gcttgctccg gtgcggtgat ctcgtcgcgg tgcggctgcg cggtcagcgt
     1081 ggcgacgttg ccggactcga acacgacgtt gccggcgttc cgcgcggtga cgtgcagcca
     1141 cacgcgccgc gacggatatc cggtaggtag cttgtggccg gtgaggttct cgactcgcac
     1201 cgtcgcgacg atggaaccgc cctcgcggtg cgaatcggtg acggtcaacc gcgccgccgt
     1261 cgcgaggtgg gcgtcgtcgc gtgcagcgct cgcgagaagc tcgctcgcat cgatgccgac
     1321 gcccgaccac tcgatcgcat cggcgagtag cgtcaggatg tacgagttgc cgccgacaaa
     1381 cgtgtgacgc ccgatcggct ggcgcggcgc gagcgacgcc ggaaagctcg cgatcggagt
     1441 cgagatcgcg cggcccgcgt cgtcgacagc gggcacgtga cagtactggc acggctggtt
     1501 cttggccggg agctcggacg cacgccattc gagatatgtc gcctgctcga cgacctcgcc
     1561 ggtcgggcgc ggcaggatga ccgtgtggca gctaccgcac agcgccgacg acgagatgtg
     1621 tgcgccgtac gtcggctcgt agttgacgat cagcatcatc ggctgtgtca tcgggttgct
     1681 gtatgcgccg aagatcttcc gctgataccc gacggcgaac ttgccggcga acgccgagtc
     1741 accgtcgagg cttcccgacg tgatctggtg gcacaacgtg catgtcacgc cgccgcgcgc
     1801 gagcgttgcc gccggcgatg ttcccgcggt gagctcgtcg aagctgacgt ggctgccgct
     1861 gtccgcgagc tcttccgaac ctgccggtcc gtggcagcgc gtgcacagct tctcgatgtc
     1921 cgctgttttc gcgggtgcgc gcgcgagctc ctcggcgacg accgcgaggt aatacggatc
     1981 gcgcgccgcg ttcgccatca gcgacgagcg ccacaacagc acgggcgaca cgttggcgcc
     2041 gtcggcatcg tggagcaccg gcgtgtcgtc ggcgacgagg tgacactgcc cgcacgcttc
     2101 agcatcgcga aagcgctccg tctcgacgtg ggctggtggc ggcaacggcg cgagcggttc
     2161 gggtcggtcc gttccggatc cagtcaccga gcacgcggcg gtcacgaaca agaaggcgac
     2221 gcgcatcgcc cacgatgcta agaggcgggt gctgaaacaa agtgcgcata ctctgcaaag
     2281 ccgcgcagaa ccagcgagtg cgacgacatg ccgctgcgac gctgcgacga cgtgtcgctt
     2341 gtcgacgcgg cgcgactaga actggccgct gagcgtgacg cccgcgctgt ggtcgctcgc
     2401 ggtcggcgtg atcgtcgtgc ccgccggtgc ggtcaagaag agatacgtcc cggcaccgcc
     2461 gatcgcgacg cccgcgacca agaacaccgt cgaccaggtc gccttcgtgt gcgcctggtc
     2521 gccgaggtcg ttcgcgcgat cgacatctgc ctgcgtcgtg cacgtgaggc cgccgcatac
     2581 ggccttcgcg tcgttccact tgctgcgcgc gagcgagccg agcacgatgc cgccgacgag
     2641 cgcggcacca ccggtcgcca ttccgccgat tccgagcagc ttcttcgtgt tgcgcggctt
     2701 gtcggggccc ttctggcgcg gggcgatacc gacggtctcg gtgttgctcg cgcccgcctc
     2761 gagcgtagcc tcgacggctg tcgtcttgcc gtcgggggcg atcttcacgc ggctcatgaa
     2821 ttcgttgtag ccacgtgcgc gcgccacgac gctgtactcg ccgacgtcga cctcgatcgg
     2881 ggtgttcgca ggaattccgc gcggtccggt cttgctgtcg atctccacaa cgattcccgg
     2941 tggcggcttg tcgaccttga cgatcagctt cgggatcttc ggcgtgatct ccttgaggcg
     3001 ctcagctgcg gtcgctttgc gctggtcgtt cgtgtccttc gtgatgagct cgcgatagcc
     3061 cgccgcggcg gtcgcgagct tgccgacctt ctcgctgcac tgcgagaggt tgaacaacgt
     3121 gccattcgcc ggatcgagct tctggctctt ctcgaattgc tcgcacgcct cggcgtactt
     3181 gccagccttg tacagatcgc ggcccgcctt gaacgcttca tctgcagccg cctgattcgg
     3241 cgatggcgtc tgcgcttgcg cggcgctggc cgccgcgacg agcgcgatca cgagaacgcg
     3301 tgcgatgcgc tcacaagcga ctgtcaccga tgtctcccgc ggtcttggtg gtctttggct
     3361 cggccttcgt cttcgatgac ttcggcgagg acgacttctt cgacgacgac ttcttcgttt
     3421 ccgccttcgc tgtcttggtc tcggcggcct tcgccgtctt ggtctcggcg gccttcgctg
     3481 tcttggtttc ggcggccttg gtctcgacag actttgtcgc tgctgctttc gtgaccgccg
     3541 gcttcgtctc cgccggcttc gtctcggccg gctttgtttc tacgggcttc gtctcggcag
     3601 cgttcgtcac gggcggctct tgcttcgccg cggctggcgc cgccgacggc gcgacttgca
     3661 tctttgtcgt gccagtcgtc gcgctggctt ctgccggcgt ggttgcgcgc gtcgggtgct
     3721 tggtcttgac gccgatcacc aggatcagcg cgagcacgat accgacgacg gcgccgctcg
     3781 cgatcacgat cggcaatcgc aggttgcgct tgttcctgat cccgctcggc gccagcactg
     3841 cgttcgcgcc gcgcagcgtg gtcggcgtgt tcgtgcccgg cgccgccgtc gacgtcggaa
     3901 gcggcgtgta ggcaccgcgc agcacccgcg cagcggcata cgccagctcg gcgccgcgca
     3961 ccggcccgac aaacggcgcg agcgcttgtg caagctcggc cacgtccttg aaccgccggt
     4021 gcggttcctt ctcgaggcag cgcaggatca cggactcgaa caacggcggc acaccgccac
     4081 cgagcggcgg cgttggatcc atcgcgacac gcaacgcgag ctccgtgatc gactcgccca
     4141 ggaacggctt gcgccccgag accagctcgt acagcacgac accgagcgac cagatgtcac
     4201 tgcgcgcgtc gacggtcttg ctcgatttga gctgctcggg agacatgtag ccaggcgacc
     4261 cgacgacgtt cgacg
//
LOCUS       DASUFX010000542         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_226774,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000542 DASUFX010000000
VERSION     DASUFX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226774"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VFV99_21635"
     CDS             complement(<1..847)
                     /locus_tag="VFV99_21635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5061992.1"
                     /translation="MSTKTTFEVTAEDEGLRLDQVIPKHVPGLSRRKTRAVIDLGGVF
                     VDKARVKVAGRPVRPGQVIEVNVGGAIERAKDPSGLAPRIVHSDDHLIVIDKPAGLVT
                     APTPESDRGDVLDQLKQQFGEVYLVHRLDLPTSGLLVFARTRAANGKLGDAFKVHDVD
                     REYRAVAVGAVTAQTIDREIDKRRAVTHVEPVETFATATLVKVQLETGRTHQIRVHLA
                     GVGHPVCGDRTHGGEVERAFLPRPPRLALHAAVLGFTHPATGERVRWESPVPDALVRW
                     IDRLRL"
     gene            complement(844..1797)
                     /locus_tag="VFV99_21640"
     CDS             complement(844..1797)
                     /locus_tag="VFV99_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5061993.1"
                     /translation="MANVEHAKRLDDSAPALPGWIEAQLPGGVRRRMVDANGETVHVM
                     EWGPADGHTVLLVHGNPTWSFLWRKVVSAIRARPGGDRLRLVAPDLVGLGLSSKPAGQ
                     AHTLEHHAAWLGAVIDQVARGPLVLAAQDWGGPIGLLAMHSRRDRLRGIVLGNTAVSP
                     PAPKFKPTLFHRLAQLPVASEVLFKRLGFPLGVLHLSQGDRSSIRGEVARAYRWPLAK
                     RADRDAPLALARMVPNHPERHPSIPAFHEIDGFFREIQVPIRIVWGKRDPVLGRVINH
                     LERLRPDATIVRTDAGHFLQEEVPEPLADAVLDVVARASWT"
     gene            complement(2015..2641)
                     /locus_tag="VFV99_21645"
     CDS             complement(2015..2641)
                     /locus_tag="VFV99_21645"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5061994.1"
                     /translation="MGTKLYVGNLNYNTTEELLREAFGANGREVASVSIIMDRETGRS
                     RGFAFVEMATAEGAQQAMQELDGQELDGRMLRINEAREREARGPGGGGGRSFGGPRPG
                     GPGGGGFGGGGPGGGGGYRGGGGGYGGGGPGGGGPGGGGGGYRGGGGGGFGGRPGGGY
                     NAGGPPGGGDGRRGRGRGGDRDRDRERGGGDDDRGKKRRGRGIDDDEY"
     gene            2850..3653
                     /locus_tag="VFV99_21650"
     CDS             2850..3653
                     /locus_tag="VFV99_21650"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX5061995.1"
                     /translation="MKKLAFLGGAAGLGMIALLNTPITQAADHLDATALATNPMADIN
                     DVYAWNTSDGMKVNLAMTISPFDNGMRNFGQSVQYVFHLTRHAAFPQTAAQLAMGEES
                     KIICTFTSNTAGECWVVDPMGTTLDYAKGDFSAVAGKDSAKGTFKVFAGRRSDPFFFN
                     LAGFLRAQDALERKCGTTTTPGACPGNLTATLDLAKCPALVPPADVQMISVHIAEEQP
                     VVVGPCAANQKDCFANANVMAIVIQIDKGQLATDEKKYLGVWASTHAGQ"
     gene            3664..4458
                     /locus_tag="VFV99_21655"
     CDS             3664..4458
                     /locus_tag="VFV99_21655"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX5061996.1"
                     /translation="MKTINFLAVVALALIGCGDNKDQPDARVRPDVAADAFMPPAVPT
                     LGAQIDRMGRPVVNTALNHGFDPTTAAQTAKVAYNEQTAKTDWLAQAQVGEFMKNLAL
                     IDVLDSGKCGNGICETGETGTNCPKPTAATDPPGDCDPAQTGAGNGCGNQVLYNGGQG
                     GPAMALSYASLAAILAEDELYLDTGKTTCSFYLGVEFGVVTGGGNSTCGGRAPEYDVI
                     DFSLSMIAMGLKGFSTDGMFTPQIKDGVDVHADVSAANFPFLGTPH"
     gene            4458..>5570
                     /locus_tag="VFV99_21660"
     CDS             4458..>5570
                     /locus_tag="VFV99_21660"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061997.1"
                     /translation="MKRLVVAVALLAACRGGKAPRNKDAGPHGSASAVSVVPKLPRTE
                     DGVAALAVLDQEIAKTTNLADSIAFRIERASIRGRVEDYQQALVDSGALVTQMPASDK
                     AWRLRVDALMRVHQFTAAREAMKELAKHIHPSFLVEYEIGLADATGDVEKAIAGREQQ
                     VKELPSATNLTLLAATLAQGGRTDEAIAMIPKAAAAVRENPPHLIAWLLFQWGRCYEL
                     KGELAAARELFAASHARLPGYLEATTHLADTMMKTGDTAGAKKLIDDALAADKTSGLT
                     PHPALLELAAQLGHPELLATARTEWERYVTALPLAFSDHAARFYLGAGKNPSRALELA
                     RMNLANRNTRDARALVIEAALAAGDAAAACAAVDPLV"
BASE COUNT          973 a         1922 c         1788 g          887 t
ORIGIN      
        1 tgagccgcag gcgatcgatc cagcgcacga gcgcgtcggg caccgggctc tcccagcgca
       61 cgcgctcgcc ggtcgccggg tgcgtgaagc cgagcacagc cgcgtgcagc gcgaggcgcg
      121 gcggtcgcgg caagaacgcg cgctcgacct caccgccgtg ggtgcgatcg ccgcacaccg
      181 gatggccgac gccagcgagg tggacgcgga tctggtgcgt gcgcccggtc tcgagctgca
      241 cctttactaa tgtagcggtc gcaaacgtct cgaccggctc gacatgggtg accgcgcggc
      301 gcttgtcgat ttcgcgatcg atcgtctggg cggtgaccgc accgaccgcg accgcgcggt
      361 actcgcgatc gacgtcgtgg accttgaacg cgtcgccgag ctttccgttc gccgcacggg
      421 tgcgcgcgaa cacgagcagg ccgctcgtcg gtagatcgag gcgatgcacg aggtagacct
      481 cgccgaactg ctgcttcagc tgatcgagca cgtcgccgcg atcggactcc ggcgtcggcg
      541 cggtgacgag gcccgccggc ttgtcgatga cgatcaggtg atcgtcgcta tgcacgatgc
      601 gcggcgcgag cccgctcggg tctttcgcgc gctcgattgc accaccgacg ttcacctcga
      661 tcacctggcc agggcgcacc ggacgaccgg cgaccttcac gcgcgctttg tcgacgaaca
      721 cgccgccgag atcgatcacc gcacgcgtct tgcggcgcga cagcccgggc acgtgcttgg
      781 gaatcacctg atcgaggcgc aggccctcgt cctcggccgt gacctcgaac gtcgtcttcg
      841 tgctcacgtc cagctcgcgc gcgcgacgac gtcgagcacc gcgtcggcga gcggctcggg
      901 gacttcttcc tgcaagaagt ggccggcgtc ggtgcgcacg atcgtcgcat cgggccgtag
      961 ccgctcgagg tgattgatca cgcgaccgag caccggatcg cgcttgcccc acacgatgcg
     1021 aatcgggacc tggatctcgc ggaaaaaccc gtcgatctcg tgaaaggccg ggatcgaggg
     1081 atgacgctcg ggatgattcg gcaccatgcg tgcgagcgcg agtggtgcat cgcgatcggc
     1141 gcgcttcgcg agcggccagc gatacgcgcg tgcgacctcg ccgcgaatgc tgctgcgatc
     1201 gccttgcgag agatgcagca cgccgagcgg aaacccgagc cgcttgaaca acacttcgct
     1261 cgcgacgggc agctgcgcga gacggtggaa cagcgtcggt ttgaatttcg gcgcgggtgg
     1321 gctgaccgcg gtgttgccga gcacgatgcc gcgcagtcga tcgcgtcgcg aatgcatcgc
     1381 gagaagtccg atcggtcctc cccagtcctg cgcggcaagc acgagcggtc cgcgcgcgac
     1441 ttgatcgatc accgcgccga gccacgcggc atgatgctcg agcgtgtgcg cctgccccgc
     1501 gggcttcgac gacagaccga ggccgacgag atcgggcgcg acgagtcgga gccgatcacc
     1561 acccggccgc gcacgaattg cgcttacgac ttttcgccac aagaagctcc aggtcggatt
     1621 tccgtgcacg aggagtacgg tgtggccgtc ggccggtccc cactccatga cgtgaacggt
     1681 ctcgccgttc gcgtcgacca tccggcgtcg gacaccgccg ggcaactgcg cttcgatcca
     1741 gccgggaagc gccggcgctg aatcatccag gcgttttgca tgctcgacgt tcgccacgcg
     1801 cggacgttac cgcgcgtcac gcgatgcgag tagcgagcgc cgacgaaacg tcactcagag
     1861 gtcgacgctt gtctgcgcgc cgacgcttgt ctacgcatcg acacttctct acgcgtcgac
     1921 gcttctctac gcatcgacgc ttctctagac gtcaacacgt ctctacgcgt caacacgtct
     1981 ctaagcgtcg acgctgctac gcgtggacgc ttctctagta ctcgtcgtcg tcgatgccgc
     2041 gaccacgacg cttcttgccg cgatcgtcat cgccgccgcc gcgctcgcga tcgcgatcac
     2101 ggtcgccacc gcgaccacga ccacgccgac cgtcgccgcc gcccggcggg ccaccggcgt
     2161 tgtatccgcc acccggacgt ccgccgaagc caccaccgcc gccgccgcga tagccaccgc
     2221 caccaccgcc gggaccgcct ccgccggggc cgccaccgcc gtagccaccg ccgccgccgc
     2281 gatagccacc gccaccgccg ggaccgcctc cgccgaagcc accgccaccc ggaccaccgg
     2341 gacgcgggcc accgaacgaa cgaccgccgc cgccaccggg tccacgcgct tcgcgttcgc
     2401 gcgcttcatt aatgcgaagc atgcgtccgt cgagctcctg accgtcgagc tcctgcatcg
     2461 cctgctgcgc accctcggcg gtcgccatct cgacgaaggc aaagccacgt gagcgtccag
     2521 tctctcgatc catgatgatc gatacgctgg ccacttcccg gccatttgcc ccgaatgctt
     2581 ccctgagtag ttcctcggtg gtgttgtagt tcagattgcc cacgtagagc ttcgttccca
     2641 cggacggtct cctgacgatc tttctgactg cgagttgctg ttccgactct gcgacaagtg
     2701 gaccggtgtc caccccacgc tctgcttcga gggtatcaca ggcgaacgga acctagatca
     2761 ccacgtgagg ggtcggaaac cgggcaggaa ttgcccccta catcgcgttt gtgcatagga
     2821 tagtttgaag ccttcacaag ggggaccgct tgaagaaact cgctttccta gggggagcag
     2881 ccgggctcgg aatgatcgcc ctgctgaata cgcccatcac acaggcagcc gatcacttgg
     2941 atgcgacggc gctcgccacc aatccgatgg ccgacatcaa cgacgtctac gcgtggaaca
     3001 cgtcggatgg catgaaggtc aacctcgcga tgaccatctc gccgttcgac aacggcatgc
     3061 gcaacttcgg tcagtcggtg cagtacgtat ttcacctgac gcggcacgct gccttcccgc
     3121 aaacagcggc gcagctcgcg atgggtgagg aaagcaagat catctgcacg ttcaccagca
     3181 acacggcggg tgagtgctgg gtcgtcgatc cgatgggcac cacgctcgac tacgcgaagg
     3241 gagatttctc ggcggtcgcg ggcaaggaca gcgcgaaggg caccttcaag gtcttcgcgg
     3301 gtcgtcgctc ggatccgttc ttcttcaacc tcgccggctt cctccgcgcg caagacgcgc
     3361 tcgagcgaaa gtgcggtacg acaacgacgc cgggggcctg ccccggcaac ctcaccgcga
     3421 cgctcgattt ggcgaagtgt ccggcgctcg ttcctccggc agacgtgcag atgatctccg
     3481 ttcacatcgc cgaggagcag ccggtggtgg tcggcccgtg tgctgcgaac cagaaggact
     3541 gcttcgcgaa cgcgaacgtg atggcgatcg tcatccagat cgacaagggg cagctcgcga
     3601 cggacgaaaa gaaatatcta ggcgtgtggg ctagcaccca cgcggggcag tgaggtgacc
     3661 accatgaaga ccatcaactt cctcgctgtc gtggccctcg cattgatcgg ctgcggtgac
     3721 aacaaagacc aacccgacgc acgcgttcga ccggacgtcg cggccgatgc gttcatgccc
     3781 ccggccgtcc cgacgctcgg cgcacagatc gatcgcatgg gtcgtcctgt ggtcaacacc
     3841 gcgctcaacc atgggttcga cccgacgacg gccgcccaga cggcgaaggt cgcctacaac
     3901 gagcagacgg caaagaccga ctggcttgcc caggcacagg tcggcgagtt catgaagaac
     3961 ctggcgctca tcgacgtgct cgactccggc aagtgcggca acggcatctg cgagactggc
     4021 gagaccggca ccaattgccc gaagcccacg gcagcgactg acccgcccgg cgactgcgat
     4081 ccggcgcaaa cgggcgccgg caacggctgc ggcaaccagg tgctgtacaa cggcggccag
     4141 ggcggcccag cgatggcgct gtcgtacgcg tcgctcgccg cgatcctcgc cgaggacgag
     4201 ctgtatctcg acaccggcaa gaccacgtgc tcgttctatc tcggcgtcga gttcggcgtc
     4261 gtcaccggcg gtggtaactc gacgtgcggt ggccgtgcgc cggagtacga cgtgatcgac
     4321 ttcagcctca gcatgatcgc gatgggtctg aagggcttct cgaccgacgg gatgttcacg
     4381 ccgcagatca aggacggcgt cgacgttcac gccgacgtca gcgcggcgaa cttcccgttc
     4441 ctgggcacgc cgcactgatg aagcgactcg tcgtcgcggt cgcgctgctc gcagcgtgcc
     4501 gcggtggcaa ggcgccccgt aacaaagacg ccggccctca cgggtcggcg tcggccgttt
     4561 cggtggtacc gaagctgccg cgcaccgagg acggtgtcgc cgcgctcgcc gttctcgatc
     4621 aagaaatcgc gaagacgacg aatctcgcgg actcgatcgc attccggatc gagcgcgcat
     4681 cgatccgcgg ccgggtcgag gactatcaac aagcgctcgt cgattcgggc gcgctcgtca
     4741 ctcagatgcc ggcgagcgac aaggcatggc ggctacgcgt cgatgcgctg atgcgcgtgc
     4801 atcagttcac cgcggcgcgc gaggcgatga aggagctcgc caagcacatt caccccagct
     4861 tcctcgtcga gtacgagatc gggctcgccg acgccaccgg tgatgtcgag aaggccattg
     4921 ccggtcgcga gcagcaggtg aaggagctac cgagcgcgac gaatctcacg ctgctcgcgg
     4981 cgacgctcgc gcagggcggc cgcaccgacg aggcgatcgc gatgattccc aaagccgcag
     5041 ccgcagtgcg cgaaaacccg ccgcacttga tcgcgtggct cttgttccag tggggccgtt
     5101 gctacgagct gaagggcgag ctcgcagcgg cgcgcgagct gtttgcagcg tcgcacgcgc
     5161 gcctgcccgg ctatctcgag gcgaccacgc acctcgccga tacgatgatg aagaccgggg
     5221 acaccgccgg tgcgaaaaag ctcatcgacg acgcgctcgc ggcggacaag acgtcgggcc
     5281 tgacaccgca ccctgccctg ctcgagcttg ccgcgcagct cgggcacccc gagctcctcg
     5341 caacggcgcg cacggagtgg gagcgttacg tgactgcgct accgctcgcg tttagcgatc
     5401 acgcggcgcg gttctacctc ggcgccggaa agaatccgag ccgtgcgctc gagctcgcgc
     5461 gaatgaacct cgctaatcgc aatacgcgcg atgcccgtgc gctcgtgatc gaggccgcac
     5521 tcgccgccgg tgacgcggcc gccgcatgtg cggctgtcga cccactcgta
//
LOCUS       DASUFX010000543         7751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1337645, whole genome shotgun sequence.
ACCESSION   DASUFX010000543 DASUFX010000000
VERSION     DASUFX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337645"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4198)
                     /locus_tag="VFV99_21665"
     CDS             complement(<1..4198)
                     /locus_tag="VFV99_21665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5061998.1"
                     /translation="MSVHPDHIRGRSAIADDGFEVGPIDTLRIPRNTVSALEAAIASG
                     APAIAQLATGEPFIDGFLELLGGGAKPALLLPVQIKSRVVAVVAAHCGDAAPNAGDVD
                     ALTPLLDASSRALARVLATREKAAGSSVVTKRTESADGYEIEVSMADVGAKRAQLDKF
                     RQAHQWAEVAEGIRELIRDGMANGDPDEDEQLELLLELGKVELDELYNPERALEAWRS
                     AQTIDAGESRLLDAIEELYVREGRWEDCIDLVEKRVALADDDQVRVATLLNLATLAHE
                     RIGDDNRAIAAYQKVLEIEPDNDLASRELGKLYTVSEQWQPLADLLLDRASRTADLDP
                     LMLVAHIFEDKLEDAHNAFVVWLTVFRREPERPHLLEQLHRLAPRTSLAELLPETKAL
                     AGELEAKYPAIAAELWHAIGNWARDVLDQREEAAAALERAARLDPGKTDEAADLLRND
                     GKWADLVALLVRRSDHETDAKRRSALFAEVGELYETRLEQPYDAIEWFERATAEDPSS
                     PAPLVALHRIHLDRENWDALGELLPRLIETLGLSAPRALIVDLYVELGTILADQLGRP
                     DDAVESFRDALALDPKHVPAFQGLAKVYQTGGQTEALLEASEAEVDASPAVVQVKRYP
                     DIAAEWHELSRLDRAIATWHKMLALEPRNVDAHKGLARALRDDEQWAELAVALRAQHK
                     VIIEPFERIALLLELADLNENRFTNHEPAISTYREVLAIDPHNRAALDALARLYERAG
                     QWLASLDILQRIAAETVNDRRAHSEVLQRIGQVQLSARDTVKAEASLTEAIALDPDNA
                     HAHEGMGRVLLQQGKLVAAAEKLLRAAQLSQHKGEITRLLTDAAWIFRYRLADNEHAR
                     ECLERILEIDPEHVDAKQAMAELLHDTQEWASLWEHLEDQAERAQTDEAMPAPERLAI
                     FQKAARCALELRKFAVSIALYDAALAIDQSPQTQLDRAEAFYRSNALDAAAAAYQTIL
                     ARYQTTLERPQLIEINRKLGAIYAELGKTGQAVTFYQKVLDLDGNHRETHNDLAELHL
                     ARGQFDDAIASLRSLAALASGPERVPFLERIGDLYHDKLSSTARAMSTYLEALDADKN
                     NHRILQRILELQTETGQWKPAVETIERFLAVETDAARRGAYHIASAEIRRTHLKDDAG
                     ALECYETAIDEMFREEPLREATRERALDAFHALRELVTADKNWKYLEQCYRRMIRRVP
                     KGDPVVLPLWDGLGDIYRFRLQHPQSAIETFEVAHNLDPDKSPHRTRILAELYAQTGT
                     KRPKQASEKAARLVEVDPMNADSYRALGRTALAAGRVDEAWCVARALVFLKQANKEET
                     ALYHRYQRHETRKAKGLLDEDAWGHVRHPDEDLTISAIFALTWEASVALRAGPAKAFQ
                     LKPKERLGIEESTGIVAKIFK"
     gene            4395..4967
                     /locus_tag="VFV99_21670"
     CDS             4395..4967
                     /locus_tag="VFV99_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5061999.1"
                     /translation="MGKRVSNDATQELSASQLVPDAKPPPKPSGNLPGVAPYGGPKND
                     ASMWMQAPVSADDFVSAPRKRPSAPSSGRGLVIGVIVVLFVLMVGAGAWYAFLRESPK
                     QSSAFDTKPAPSTPNTSVVPAPPDAALAVAAPDAAAVAATPDAGTADQALAADAVSGA
                     GPVQKKATKRATTKKKVTKKKTATAPKRRK"
     gene            4977..5807
                     /locus_tag="VFV99_21675"
     CDS             4977..5807
                     /locus_tag="VFV99_21675"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HEX5062000.1"
                     /translation="MRSLLVLVVVAACGNPSGGTPDGAMGDDDAQAMPDGLLPVKLPP
                     LNDKVDYQLGGAYAPPSGVKIVSRDRKATRADGLYNICYVNGFQIQPDEEDWWLTNHP
                     DLILRDASNNPVIDTQWNEMLLDTRLAEKRTAIASIVGMWILGCAQTSYQAVEIDNLD
                     SYSRSQGLLTQDQAVMTMKMMADHAHATGLAIAQKNSSELVARKAELGTDFAVAEECN
                     RYNECDVYTGGYGDHVIVIEYRRQDFDAGCTAFPNLSIVLRDVNLVTPQGSGYVYDGC
                     "
     gene            5807..7540
                     /locus_tag="VFV99_21680"
     CDS             5807..7540
                     /locus_tag="VFV99_21680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5062001.1"
                     /translation="MDTANIRDGLAKLGVKRLLFAIHDASFPSDPDEDIGRGSPSTKA
                     AARLFCYVRDLGFTGIQLGPQGQTSRGNPSPYDGTIFSRNVANIGIASFRTGGPFEGL
                     LAQRDLEEALVDGFGPARHRHAYDESHRLVGKAYGAIQRGARPDLAARIGEFAKANFR
                     WLLSDALHAALHAEWQAGFRAWPTRDREVWEKNDLERIKQLIDQHEREIDRYSFGQML
                     AHEEHERVRRICHMQLYGDMQIGYSDADAWRHQRAFLLGYLMGAPPSRTNPEGQPWGY
                     PVLDPGLYESDAGALVLSRAMKAFAEYDSLRIDHPHGLVCPWVYRSDTDDPAQAVRDG
                     ARLFESPDLPDHPVLGVFSIVRPDQIDRTKPRYHDAWVTDLDDDQVDRYSILIDAILD
                     TAERAGRTRDDLCCEVLSTMPTPLAAVLARHGLGRFRVTQKANLDDATDVYRAENARP
                     EDWMMLGNHDTPPIFAVIRGWSPAQREKWSRHLAARLKLRHPERLVSARFTASAMLAE
                     LFASRAENVSIFFADLFGLEDRFNVPGVIDDKNWTLRLPPDFEALHASQLANEHALDL
                     PLAIELALAAG"
     gene            complement(7537..>7751)
                     /locus_tag="VFV99_21685"
     CDS             complement(7537..>7751)
                     /locus_tag="VFV99_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5062002.1"
                     /translation="NFTPVPRPNYRVGVPSAGLWSEILNTDAQELGGSGHGNLGSIEA
                     APVRSHDRPFSLSLTVPPLGAVFVRR"
BASE COUNT         1252 a         2539 c         2615 g         1345 t
ORIGIN      
        1 gcttgaagat cttcgcgacg atgccggtgc tctcctcgat cccgaggcgc tcctttggct
       61 tgagctggaa cgccttcgcc gggccggcgc gcagcgcgac cgaggcttcc caggtgagcg
      121 cgaagatcgc gctgatcgtc agatcctcgt cggggtgacg aacgtgtccc cacgcatctt
      181 cgtcgagcag ccccttcgcc ttgcgcgttt cgtgacgctg atagcggtga tagagcgccg
      241 tctcttcctt gttcgcttgc ttgaggaaca cgagcgcacg cgcgacgcac catgcttcat
      301 cgacgcggcc agcggcgagc gccgtgcgac cgagcgcgcg atagctgtcc gcattcatcg
      361 gatcgacttc gacgagtcgc gcggccttct ccgacgcttg tttcggccgc ttggttccgg
      421 tctgcgcgta cagctcggcg aggatgcgcg tgcgatgcgg cgatttgtcc ggatcgagat
      481 tgtgtgcgac ctcgaacgtc tcgatcgcgc tctgcggatg ctggaggcgg aagcgataga
      541 tatcgccgag gccgtcccac agcgggagca cgaccggatc gcccttcggc acgcgccgga
      601 tcatgcggcg gtagcactgc tcgagatact tccagttctt gtcggcggtc acgagctcgc
      661 gcagcgcgtg gaacgcgtcg agtgcacgct ctcgagtcgc ttcgcgcaac ggctcttcgc
      721 ggaacatctc gtcgatcgcc gtctcgtaac actcgagcgc accagcgtcg tccttgaggt
      781 gcgtgcggcg aatctcggcc gaggcgatgt ggtacgcgcc gcggcgcgcc gcatcggtct
      841 cgacggcgag gaagcgctcg atcgtctcga cggccggttt ccactggccg gtctccgtct
      901 gcagctcgag gatgcgctgg aggatgcggt ggttgttctt gtcggcgtcg agcgcttcga
      961 gatacgtcga catcgcgcgc gctgtcgacg acagcttgtc gtgatagaga tcgccgatgc
     1021 gctcgaggaa cgggacgcgt tcaggacccg atgccagtgc agcgagcgag cgcaggctcg
     1081 cgatggcgtc gtcgaactgg ccgcgtgcga gatggagctc ggcgaggtcg ttgtgggtct
     1141 cgcggtgatt accgtcgagg tcgagcacct tttgatagaa cgtgacggct tggcccgtct
     1201 tgccgagctc tgcgtagatc gcaccgagct tgcggttgat ctcgatcagc tgcgggcgct
     1261 cgagcgtggt ctggtaccgc gcgaggatcg tctggtacgc ggctgcggcc gcatcgagcg
     1321 cgttgctgcg atagaacgcc tcggcgcggt cgagctgcgt ctgcggtgat tgatcgatcg
     1381 ccagcgcggc atcgtagagc gcgatcgaga ccgcgaactt gcgcagctcg agtgcgcagc
     1441 gagccgcctt ctggaagatc gcgagccgct cgggcgccgg catcgcctcg tcggtctgcg
     1501 cgcgctcggc ttggtcctcg agatgctccc acagcgacgc ccactcctgg gtatcgtgga
     1561 gcagctcggc catcgcttgc ttggcgtcga cgtgctcggg atcgatctcg aggatgcgtt
     1621 cgaggcactc gcgcgcgtgc tcgttgtcgg cgagccggta gcggaagatc cacgctgcat
     1681 cggtcagcag gcgcgtgatc tcgcctttgt gctgcgacag ctgcgcagcg cggagcagct
     1741 tctcggcggc ggcgacgagc ttgccctgct gcagcagcac gcggcccatg ccctcgtgcg
     1801 cgtgggcatt gtcggggtcg agcgcgatcg cttcggtgag cgacgcttcc gccttcacgg
     1861 tgtcgcgcgc gctgagctgc acctgaccga ttcgttgcag gacctcgctg tgggcccggc
     1921 gatcgttgac ggtttcggcg gcgatgcgct gcaggatgtc cagcgacgcc agccactggc
     1981 cggcacgttc gtagaggcgc gcgagcgcgt cgagcgcagc gcgattgtgt gggtcgatcg
     2041 cgagcacctc gcggtacgtc gagatcgcag gctcgtggtt cgtgaaccgg ttctcgttca
     2101 gatcggcgag ctcgagcaac agcgcgatgc gctcgaacgg ctcgatgatc accttgtgct
     2161 gcgcgcgcag tgcgaccgcg agctcggccc actgttcgtc gtcgcgcagg gcacgcgcga
     2221 ggcccttgtg cgcgtcgacg ttgcgcggct cgagcgcgag catcttatgc cacgtcgcga
     2281 tcgcgcgatc gaggcgcgat agctcgtgcc actccgccgc gatgtcggga tagcgtttga
     2341 cctgcacgac cgcaggcgag gcatcgacct cggcttcgct cgcctcgagc agtgcctcgg
     2401 tctggccgcc ggtttgatag acctttgcga ggccctgaaa cgcgggcacg tgcttcgggt
     2461 cgagcgcgag cgcgtcgcgg aacgactcga cggcgtcgtc cggacggccg agctggtccg
     2521 cgaggatcgt accgagctcg acatagagat cgacgatcag cgcgcgaggc gccgagagac
     2581 cgagcgtctc gatgaggcgc ggcaacagct cgccgagcgc gtcccagttc tcgcggtcga
     2641 ggtggatgcg gtgcagcgcg acaagcggcg ccggcgacga cggatcctcg gcagtggcgc
     2701 gctcgaacca ctcgatcgcg tcgtacggct gctcgagcct ggtctcgtac agctcgccga
     2761 cctcggcgaa cagcgcgctg cgccgcttcg catcggtctc gtgatcgctg cggcggacga
     2821 ggagcgcgac gaggtcagcc cacttgccgt cgttgcgaag caggtcagcc gcttcgtcgg
     2881 tctttcccgg atcgaggcgt gcagcgcgct cgagcgcggc tgccgcttcc tcgcgctggt
     2941 cgaggacatc gcgcgcccag ttgccgatcg cgtgccatag ctcggcggcg atcgccggat
     3001 acttcgcttc gagctcgccg gcgagcgcct tggtctcggg caatagctcg gcgaggctcg
     3061 tgcgcggcgc gagccggtgc agctgttcga gcaggtgcgg tcgctcgggt tcgcggcgga
     3121 acaccgtgag ccacacgacg aacgcgttgt gcgcgtcttc gagcttgtcc tcgaagatgt
     3181 gcgcgacgag catcagcgga tcgagatcgg ccgtgcgcga ggcgcgatcg agcaacaggt
     3241 cggcgagcgg ctgccattgc tcgctcaccg tgtacagctt gccgagctcg cgcgaggcga
     3301 ggtcgttgtc gggctcgatc tcgaggacct tctggtacgc cgcgatcgcg cggttgtcgt
     3361 cgccaatgcg ctcgtgcgcg agcgtggcca ggttcaacag cgtcgcgacg cgcacttgat
     3421 cgtcatcggc aagcgcgacg cgcttctcga cgagatcgat gcagtcctcc caacggcctt
     3481 cgcggacgta gagctcctcg atcgcgtcga gcaggcgcga ctcaccggcg tcgatcgtct
     3541 gcgcgctgcg ccacgcctcg agcgcacgct ccgggttgta cagctcgtcg agctctacct
     3601 tgccgagctc gagcaatagc tccagctgct cgtcctcgtc gggatcgccg ttcgccatgc
     3661 cgtcgcggat cagctcgcgg atgccttccg caacctctgc ccactgatgc gcctggcgaa
     3721 acttgtcgag ctgggcgcgc ttcgccccga cgtcggccat cgagacctcg atctcgtagc
     3781 catcggcgct ttcggtccgc ttcgtgacca ccgacgagcc agcggccttc tcccgggtcg
     3841 cgaggacgcg tgcgagtgcg cggctcgatg catcgagaag cggcgtcagc gcatcgacat
     3901 cgccggcgtt cggcgcagca tcaccacaat gcgcggcgac gaccgccacc acgcgcgatt
     3961 tgatctgcac gggaagcagc agtgcgggct tggcgccgcc gccgagcagc tcgagaaagc
     4021 cgtcgatgaa cggctcgcca gtggcgagct gcgcgatggc cggcgccccg gaggcgatcg
     4081 ccgcctcaag cgccgatacg gtgttgcgag ggattcgcaa cgtgtcgatg ggaccaacct
     4141 cgaagccgtc atctgcaatc gcgctgcgcc cgcggatgtg gtcgggatgg accgacaaca
     4201 gcgccgcaaa tcttgccttc gaccgggccg cccgcaggag cagatcgaag acctgttggc
     4261 ggtcgctggc accctcgatt gcgcttgcgg cacgagccgg gtcaagcggc gattgccctg
     4321 taagttccac cggcactggg aagcgatggt acttccggtg ttgagcgcgc gcgagtggta
     4381 agctcgtcgc gaagatgggc aaacgcgtca gtaacgatgc gacgcaagag ttgtcggcga
     4441 gccagctcgt gcccgacgcg aagccgcctc cgaaaccttc ggggaacctg cccggggtgg
     4501 ccccgtacgg tggcccgaag aacgacgcga gcatgtggat gcaggcgccg gtcagcgccg
     4561 atgacttcgt ctcggcgcct cgcaaacgac cgtctgcacc gagcagcggt cgcggtctcg
     4621 tgatcggcgt gatcgtcgtg ttgttcgtgc tgatggtcgg cgccggtgct tggtacgcgt
     4681 tcttacgcga atcaccaaag cagtcgtcgg cgttcgacac gaagcccgca ccgtcgacgc
     4741 cgaacacatc ggtcgtaccg gcgccgccgg atgctgcgct tgcggtcgcg gcgccagatg
     4801 ctgcggcggt tgcagccacc cctgacgccg gtactgccga ccaggccctc gcggccgatg
     4861 cggtgtcggg tgccggcccg gtgcagaaaa aagcgaccaa acgcgcgaca acgaagaaga
     4921 aagtcacgaa gaagaaaacc gcgacagcgc caaagcgacg caagtagtat cggcgcgtgc
     4981 gtagtctcct cgttcttgtt gtcgtagcgg cgtgtggcaa tccgtccgga ggaacgccag
     5041 acggcgcgat gggtgacgac gacgcacaag cgatgcccga cggcttgctc ccagtgaagc
     5101 tgccgcccct gaacgacaag gtcgactacc agctcggcgg tgcttacgcg ccgccgtccg
     5161 gcgtgaagat cgtatcgcgc gaccgcaaag cgacgcgcgc ggatggcctc tacaacatct
     5221 gctacgtcaa cggctttcag atccagcccg acgaggagga ctggtggctc accaatcatc
     5281 cggatctgat cctgcgcgat gccagcaaca atccggtgat cgacacgcag tggaacgaga
     5341 tgctgctcga caccaggctc gccgagaagc gcaccgcgat cgcgtcgatc gtcggcatgt
     5401 ggatcctcgg gtgtgcgcag accagctatc aggcggtcga gatcgataac ctcgattcct
     5461 attcgcgctc gcaaggcctg ctcacgcaag accaagcggt gatgacgatg aagatgatgg
     5521 ccgaccatgc gcacgcgacc ggcctcgcga tcgcgcagaa gaactcgtcg gagctcgtcg
     5581 cgcgcaaggc cgagctcggc actgacttcg cagtcgccga ggagtgcaac cgctacaacg
     5641 agtgcgacgt gtacaccggc ggctacggcg accacgtcat cgtgatcgag tatcgccgcc
     5701 aggatttcga tgcgggctgc accgcgttcc ctaacctgtc gatcgtgctg cgcgatgtga
     5761 atctggtgac gccgcagggc tccgggtacg tctacgacgg ctgctgatgg acactgcgaa
     5821 catccgcgac ggtctcgcga agctcggcgt aaagcggctg ctgttcgcga tccacgacgc
     5881 gagctttccg tcggatcccg acgaggacat cgggcgcgga tctccgtcga cgaaggccgc
     5941 cgcgcgcttg ttttgctacg tgcgcgacct cggcttcacg ggcattcagc tcgggccgca
     6001 gggccagacc tcgcgcggta acccgtcgcc ctatgacggc acgatcttct cgcgcaacgt
     6061 cgcgaacatc ggcatcgcat cgtttcgcac cggtggcccg ttcgaggggc tcctcgcgca
     6121 acgcgacctc gaggaggcac tcgtcgacgg gttcgggccg gcacgtcatc ggcatgccta
     6181 cgacgaaagc caccggctcg tggggaaagc gtacggggcg atccaacgcg gcgcgcgccc
     6241 cgacctcgct gcgcgaatcg gcgagttcgc gaaggcaaac ttcaggtggc tgctgtcgga
     6301 cgcgctgcac gcagcacttc atgccgagtg gcaggcaggc tttcgcgcat ggcccacgcg
     6361 cgatcgcgag gtatgggaga agaacgatct cgagcgcatc aagcaactca tcgatcaaca
     6421 cgagcgcgag atcgatcgct actcgttcgg ccagatgctc gcacacgagg agcacgagcg
     6481 cgtgcgccgc atctgtcaca tgcagctcta cggcgacatg cagatcggct actccgacgc
     6541 cgatgcgtgg cgtcaccagc gcgcgttcct gctcggctac ctgatgggcg cgccgccgag
     6601 ccgaacgaat cccgaggggc agccgtgggg ctatccggtg ctcgatccgg ggctctacga
     6661 gtcagacgcc ggcgcgctcg tgttgtcgcg cgcgatgaag gcattcgccg agtacgacag
     6721 tctgcgcatc gatcatccgc acggcctcgt ctgtccgtgg gtgtatcgca gcgacaccga
     6781 cgatcctgcg caagcggtcc gcgatggcgc gcgtctgttc gagtcgccgg atcttcccga
     6841 ccatccggtg ctcggtgtgt tttcgatcgt gcgccccgat cagatcgatc gcacgaagcc
     6901 gcgctaccac gacgcgtggg tcactgacct cgacgacgac caggtcgacc gttactcgat
     6961 cttgatcgac gcgatcctcg acaccgccga gcgcgccggt cgcacgcgtg acgatctgtg
     7021 ctgcgaggtg ctgtcgacga tgccgacgcc gctcgctgct gtgcttgcgc gccacggact
     7081 cggccgcttc cgcgtcaccc agaaggcgaa cctcgacgac gcgaccgacg tctaccgcgc
     7141 ggagaacgcc aggcccgaag actggatgat gctcggcaac cacgacacgc cgccgatctt
     7201 cgcggtaatt cgtggctggt caccagcgca gcgcgagaag tggtcgcgtc acctcgcagc
     7261 acgcctgaag ctccgccatc ccgagcgcct tgtcagcgcg cggttcaccg cgagtgcgat
     7321 gctcgccgag ctgttcgcct cgcgtgccga gaacgtgtcg atcttctttg ccgacctgtt
     7381 cgggctcgag gaccgcttca acgtgccggg cgtcatcgac gacaagaact ggacgctgcg
     7441 gctgccgccc gacttcgagg cgctgcatgc gagccagctc gcgaacgaac acgcgctcga
     7501 tctaccactc gcgatcgagc tcgcactcgc ggcgggctag cggcgcacaa agaccgcacc
     7561 gagcggcgga accgtgagcg atagcgagaa cgggcgatcg tgggagcgca ccggcgctgc
     7621 ttcgatacta ccgaggttgc cgtgaccgct accgccgagc tcctgcgcgt cggtattgag
     7681 gatttcgctc cacaaacccg cgctcggaac gcccacgcgg tagttggggc gtggcaccgg
     7741 cgtgaagttc a
//
LOCUS       DASUFX010000544        10539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_505574,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000544 DASUFX010000000
VERSION     DASUFX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505574"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..544
                     /locus_tag="VFV99_21690"
     CDS             182..544
                     /locus_tag="VFV99_21690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5062003.1"
                     /translation="MHERRLGYRIPYESMVTSYVHDRPIRGLATNLSDSGLNMSAISM
                     VAPPPGMVVGLEIEIPGMDESIWASGRICYRKDDRLASGLGVRFVAMAKTHARTLRDF
                     CIEMRRKNLGSILARIRA"
     gene            613..4614
                     /locus_tag="VFV99_21695"
     CDS             613..4614
                     /locus_tag="VFV99_21695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5062004.1"
                     /translation="MRRLRLLLALALASATVSPALAQPGDWGVTRDPFDKTVVARYKA
                     ILAKNPHDASALAKLLEMYRRYKTVDLLKDEYQKSLDKNGNDWAALVVMGRLHRMIGD
                     DQRARDFLAKAVGAKDSDAQSWILIGEIDKNGGKNQDARTAYDKALSHATTKDMKKKA
                     LRALADLALATGDNEGANNYFKKFLELDPNNAQLWLERGDAMLAAGKRDVALESYAAA
                     EKLLGSDPAKRVEVVARRGQALEAMGKDDEAVTEYRRAIKLAPKGYYLEVELTGRIVD
                     IYRRKQALPQLLTQYEKEWPEGSRGHFEWNTLGRLYEETGSQDKAIAALKKAVAKSPF
                     ELETQRRLIQLLENSGRDDEALAQYEAVVRAAPGEARFQLELAERYWRRGLEKKALDS
                     LTRLQGRFPNDAGVLSAIADLYTRWGKDDLAIAQYERLAKLEPDDPAHLVTLGEQYWQ
                     KGDPASKAKATATWNRLAATKKPIGYAKLAEVLAEHGRTTEAMSNYDTAIKLDDKNPE
                     FFKGRATLWESQKQYVNARKDWESALALIGRKASDRLARRDATRHIVQTLARDSSSRQ
                     AEYLTKWTTAFAKTPPDADAGYFLIEYYTRKNQDKELRQTLEKMLAAVPDDSELLLDL
                     VKVYRKLRKYDEAVALLLELAKKQPSQERQVYQQISEIKTEARKDDEAIEWAQKALAK
                     NPTDPGAYERLGERYVEMQRFGDAIAAYEKTVQLDPRNSKAAFALSQLYVQNGTPMKA
                     AELLRNVLRQTSDDDVVDRAGNQAIDLEEMTDTLGELEKVISPLSFMMAHKPVYRRVL
                     VKLYLRYVPRLVERSRHGTEEVRKAARLELDRVGARGLRPLLEALRDQKDTAQQRIAV
                     QVLGHLGNKGAAAPLVHMARQEPPKDQRHIGTLAEGLDREVRVDALVAAGRLGDAKVI
                     DDVLPLMDHQESAMREAATFTLGRSGDKRAVAALLKTLSDRKEREQVLACLGLAQIDD
                     KRVGPALIAALKDASKVDAVRAACAYAIGVRKITAGVPALLAAVDDNRGETQRLAAWS
                     LGQLGETKALGPLIRAYFARAGQGADELVWSIGRVSGANLAAAPAGNFSEFPMRSGKY
                     DQFAALRGLPGPLPMPPANPKLVVDHAKDIATGLTQALAEHRDVVVSVLADLDSAPDR
                     LALGALTPATLDAKTTAAVTSIAQAIEPQVTAQLSSEDPKVRALAVSVTAKLDSGKGG
                     KADSAITRALSDPSDQVRAAAMQSIAVLARRRGSASPELVGALTKTLGSASWSDRRVA
                     ALSLGKLGSAGDPQALAKAAKDSSSFVREAVAVALGEIGGPQVVETLQMLAKDEVVQV
                     REAATHALSTLKR"
     gene            4682..5674
                     /locus_tag="VFV99_21700"
     CDS             4682..5674
                     /locus_tag="VFV99_21700"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5062005.1"
                     /translation="MDVGLVCDACSALTPIGVPQCARCGQPVALDPRPRRTSKPSGPN
                     GGATAAAAAQGAGTPCGKCGTTVPSNFKFCQICGTRVVVAQDFQVETRVGPRQSQTAD
                     AQKPGRSTLFFGGAAQAARAKLTLIRGDGEDGVSFTLAGTDHLAGRGECPISFPDDPY
                     LSPIHCNFRYAKNTLVVHDEGSLNGVFVRISGSVKIEPGSTVLVGEQVLTVSPARGFD
                     DLPDAEGTYYSASMMRPATIEIVQQLRGGSCGWVFRPDTEMVSIGREGNDINFPDDPF
                     ISGKHAQLQLTGGVLTVTDLGSRNGTFVRVTGEHVLKHGDYVFLGQQLLRVEIV"
     gene            5707..6843
                     /locus_tag="VFV99_21705"
     CDS             5707..6843
                     /locus_tag="VFV99_21705"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5062006.1"
                     /translation="MTVCNRCGKENQDHYKFCLGCGAELTAPKPGGGGDMGMMKTMMA
                     DPGAGAGAPLGGPRGPSGAMPGQPMRPSGGGMPGMPPSGPLGGAPMAPPPMAPPMGPP
                     PGVPSYGNFPPPAAAPAGGMRPCPSCGSGVPPSFRFCGTCGFRMDEQSAPAPMPQPPM
                     GGGFAPQPQRSRLSMTLIRPDGTEGGTHDLRPGENKLGRSFGPVFENDGYLSPVHALL
                     DIRGVQAVVRDLDSLNGVFVKMTQEEELQSGQIIRIGQELLRFEIIPTPEPTADGTEL
                     MGSPNPGFWGKLTVIIGRDVTGAAFPLLGESVTLGRERGEINFPDDGYVSGLHARISL
                     REGRVFLADLGSSNGTFIKVNGERNVGHESFVLLGQQLFRLNFS"
     gene            complement(6892..7290)
                     /locus_tag="VFV99_21710"
     CDS             complement(6892..7290)
                     /locus_tag="VFV99_21710"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5062007.1"
                     /translation="MTRRLPVLPLLLVIVLAACGSEDTKLKVTGLEPEKGDVNGGTYV
                     RIKGNRFIADGARNAKVYFGSRQGTVVRFASDSELIVEAPGGKPNEQVDVLIIFEPGG
                     ELKIAKGFTFVEKQESTMDVKDLDTSKIKK"
     gene            complement(7365..8693)
                     /locus_tag="VFV99_21715"
     CDS             complement(7365..8693)
                     /locus_tag="VFV99_21715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5062008.1"
                     /translation="MAAIVSKLPQALQQTARKSRVARVTAKRPKHEEPESDVELDGEE
                     EVEAAEKPARPDNGTYLSMYFRDMAELDVLRPEEEFTSAREIEALEIMLWEAVLSHPQ
                     AIERILEAVATVPDFVMPLEAKTLRRMASERDAKAFARASARAARKLRSGDVDHLFIE
                     AALGAIDRMTLGIGVRGSAVGALGQRTKVAEWLRRVSQANRAAADARNDFVKANLRLV
                     VSIARRFNHGRMALADLIQEGNIGLMKAVERYDYRRGFRFSTYASWWIRHAISRALAD
                     KGREVRLPVHMIDAQHRLTKARRQLTGKLGRQPTTEELAEATNMPLDKVEKMRSWLLE
                     QSVSIDKPVGDDEGRVLGEVLEDPDREAVSPTEDLEWEALTTEVRELLRELRPIEADI
                     LRQRFGLGTEQELTLKEIGDKYNLSRERIRQLQEQALAKMRRALARRDLL"
     gene            complement(8810..>10539)
                     /locus_tag="VFV99_21720"
     CDS             complement(8810..>10539)
                     /locus_tag="VFV99_21720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5062009.1"
                     /translation="PRAVHNEISAPAPIAPDAVPTVIDPPNPQASEDFEMKTREMSAV
                     DLEALGLSTSDTVSSAYSQLSSPGMPAMRGERTVDVDVDVTLDTETPDPDREPVTAQR
                     PAIEDPFSKQTRQIQAPTTGGAKKLPGSRHKTVALTEEDLEEVRAATQPVAAKPKAAD
                     SNRTMPLAAIDVEVLTKSPASEEPMSVPDIDIQELADRAESSGEIEVDVDEDKADEKV
                     IEKVAEPPPSTRVSTPSVPPQIRNQSAPTIDRKSGAIVEAQHSPPAQHPPPAPTAQVS
                     KEHHPPPTPPAAAHKPPPAPPPAKKPIPQAAVAAVVAKEPSKPTSGRAKPWFVDLFDE
                     DYLRTLPFLTPQATQAEAEFVINAMNLAPGAQVLDLGCGYGRHAMELAARGFHVVGLD
                     LSTPLLVRGGEEAHRRGLTINFVRGDMRELDFENQFDGVYCLFSTFGYFDDETNKKTL
                     QNVARALKPNGKVLVEILNRDYVIADLPTRVWWEGDGCVVLEEVELNYFSSRIQVNRS
                     VVFDDGRQLEQEISVRAYSLHEVGKLMHAAGFRVLEVSGGYQMRGRFFGNQSRHIIVL
                     AERKETSGT"
BASE COUNT         1818 a         3474 c         3496 g         1751 t
ORIGIN      
        1 cttgtctggg cgcgtcccgt ctgcacgaag ccagcgggcg acgagtgatt gagcgcgcgc
       61 ctggtcgcgc gtcagctttg gcagccgatg ccacgggtac gccgaaaagt tgggcgtcga
      121 aaccaagctg gtacgatttt atcccacatc cgcccaccag aggggagcaa gagctcaaac
      181 catgcacgag cgccgtttag gctaccggat cccatacgag tcgatggtca cttcctacgt
      241 ccacgatcgt cccatacgcg ggctcgcgac gaacctctcc gatagcgggc tcaacatgtc
      301 cgcgatctcg atggtggcac caccgcccgg gatggtcgtc gggctcgaaa tcgagattcc
      361 tggaatggac gagtcgatct gggcgtctgg tcgaatctgc tatcgcaagg acgaccggct
      421 cgcctcgggt cttggcgtgc gcttcgtcgc gatggcgaag acgcacgcgc gcacgttgcg
      481 tgatttctgc atcgagatgc gccgcaagaa cttgggctcg atcctggcgc gtatccgcgc
      541 gtaagcgatc ggttttccgc ccggattccg acgggcactc gcggcagcca gcggctatga
      601 tcgacggaag gtatgaggcg cctccgtcta ttgctcgccc tcgcgttagc tagcgccacg
      661 gtctcccccg cgctcgcaca gcccggtgac tggggcgtca cgcgcgaccc gttcgacaag
      721 acggtcgtcg ctcgttacaa ggccatcctc gcgaagaacc cgcacgatgc gtcggcgctc
      781 gcgaagctgc tcgagatgta tcgccggtac aagaccgtcg atctgctcaa ggacgagtac
      841 cagaagtcgc tcgacaagaa cggcaacgac tgggccgcgc tcgtggtgat gggccggttg
      901 caccggatga tcggcgacga tcaacgcgcg cgagacttct tggccaaggc ggtcggcgcg
      961 aaggacagcg acgcgcagag ctggatcctg atcggcgaga tcgacaagaa cggcggcaag
     1021 aaccaggacg cacgtaccgc ctacgacaag gcgctatcgc acgcgacgac gaaggacatg
     1081 aagaagaagg cgctgcgtgc gctcgccgac ctcgcgctcg cgaccggcga caacgaaggc
     1141 gcgaacaact acttcaagaa gtttctcgag ctcgatccga acaacgcaca gctatggctc
     1201 gagcgcggtg acgcgatgct cgcagctggt aagcgtgacg tcgcactcga gagctatgcc
     1261 gctgccgaaa agcttctcgg ctccgatccg gccaaacgcg tcgaggtcgt cgcgcggcgc
     1321 ggtcaggcgc tcgaggccat gggcaaggac gacgaggccg tcaccgagta ccgccgcgcg
     1381 atcaagctcg cgccgaaagg ctactacctc gaggtcgagc tgaccggccg catcgtcgat
     1441 atctatcgcc gcaagcaggc gctaccgcag ctcctcacgc agtacgagaa ggagtggccg
     1501 gaaggctcgc gcggtcactt cgagtggaac acgctcggcc ggctctacga agagaccggc
     1561 tcgcaggaca aggcgatcgc ggcgctcaag aaagcggtcg cgaagtcgcc gttcgagctc
     1621 gagacgcagc gccggttgat ccagctgctc gagaactcgg gccgcgacga tgaagcgctc
     1681 gcgcaatacg aagccgtcgt gcgcgcagcc cccggcgagg cgcgctttca gctcgagctt
     1741 gccgagcgct actggcgccg cggcctcgag aagaaggcgc tcgattcgct gactcggctg
     1801 cagggccggt tcccgaacga cgccggcgtg ctgtcggcga tcgccgatct gtacacgcga
     1861 tggggcaagg atgacctcgc gatcgcgcag tacgagcgcc tcgcgaagct cgagcccgac
     1921 gatccggcgc acctcgtgac gctcggcgag cagtactggc aaaaaggcga tccggcgagc
     1981 aaggcgaagg cgaccgcgac ctggaaccgg ctcgctgcca cgaagaagcc gatcggctac
     2041 gcgaagctcg ccgaggtgct cgccgagcac ggccgcacga ccgaagcgat gtcgaactac
     2101 gacacggcga tcaagctcga cgacaagaac cccgagttct tcaaaggccg cgcgacgctg
     2161 tgggagagcc agaagcagta cgtcaacgcg cgcaaggact gggagtccgc gctcgcgctg
     2221 atcggacgca aagcgagcga ccggctcgcg cgtcgcgatg ccacgcgcca catcgtgcag
     2281 acgctcgcgc gggattcgag ctcgcgccag gccgagtacc tgacgaagtg gacgacggca
     2341 ttcgccaaga cgccgcccga tgccgacgcc ggctacttcc tcatcgagta ctacacgcgc
     2401 aagaaccagg acaaagagct gcgccagacg ctcgagaaga tgctggcggc ggttcccgac
     2461 gatagcgagc tgttgctcga cctcgtgaag gtctaccgca agctccgcaa gtacgacgaa
     2521 gccgtcgcgc tgctgctcga gctcgcgaag aagcagccgt cgcaggagcg ccaggtctat
     2581 cagcagatca gcgagatcaa gaccgaggcg cgcaaggacg acgaggcgat cgagtgggcg
     2641 cagaaggcgc tcgcgaagaa tccgaccgat ccaggcgcgt acgagcgtct cggcgagcgc
     2701 tacgtcgaga tgcagcgatt cggcgacgcg atcgcggcgt acgagaagac ggtccagctc
     2761 gatccgcgca acagcaaggc cgcgttcgcg ctgtcgcagc tctacgttca gaacggcacg
     2821 ccgatgaagg cagccgagct cttgcgcaac gtgctgcgcc agaccagcga cgacgacgtc
     2881 gtcgatcgcg cgggcaatca ggcgatcgat ctcgaagaga tgaccgacac gctcggcgag
     2941 ctcgagaaag tgatctcgcc gctgtcgttc atgatggcgc acaaaccggt gtaccggcgc
     3001 gtgctcgtga agctctacct ccgctacgtg cctcggctcg tcgagcgctc gcgccacggc
     3061 acggaagagg tgcgcaaggc agcgcggctc gagctcgatc gtgtcggtgc acgcggtctg
     3121 cgtccgttgc tcgaagcgct gcgcgatcag aaggacaccg cgcagcagcg catcgccgtg
     3181 caggtgctcg gtcacctcgg caacaagggc gccgctgcgc cgctcgtcca catggcgcgc
     3241 caggagccgc cgaaagatca gcggcacatc ggcacgctgg ccgagggcct cgatcgcgaa
     3301 gtccgcgtcg acgcgctcgt cgcggctggc cggctcggcg acgcgaaggt catcgatgac
     3361 gtgctgccgc tcatggatca ccaagagagc gcgatgcgcg aagcggcgac gttcacgctc
     3421 ggccgctccg gcgacaagcg cgcggtggcg gcgctgctca agacgctctc cgatcgcaag
     3481 gagcgcgagc aggtactcgc gtgcctcggc ctcgcgcaga tcgacgataa gcgcgtcggt
     3541 cctgcgctga tcgcggcgct caaggacgcg agcaaggtcg atgcggtgcg cgcggcgtgc
     3601 gcgtacgcga tcggcgtccg caagatcacc gcgggtgtcc ctgccctgct tgctgccgtc
     3661 gacgacaacc gcggcgaaac tcagcggctc gcagcgtggt cgctcggtca gctcggcgag
     3721 acgaaggcgc tcggcccgct gatccgcgcc tacttcgcgc gtgctggcca aggcgccgac
     3781 gagctcgtgt ggtcgatcgg ccgtgtcagc ggcgccaacc tcgccgctgc ccctgccggc
     3841 aacttcagcg agttcccgat gcgcagcggc aagtacgacc agttcgcggc gctccgcggg
     3901 ctgcccggcc cgctgccgat gccgccggcg aatcccaagc tcgtcgtcga tcacgcgaag
     3961 gacatcgcga ccggtctcac ccaggcgctc gccgagcatc gcgacgtcgt ggtgtcggtg
     4021 ctcgccgatc tcgacagcgc ccccgaccgg ctcgcgctcg gcgcgctgac gccggcgacg
     4081 ctcgacgcca agaccaccgc ggcggtcacc agcatcgccc aggcgatcga gccgcaggtc
     4141 accgcgcagc tgtcgtccga ggatccgaag gtccgcgccc tcgcggtgtc ggtcaccgcc
     4201 aagctcgaca gcggcaaggg cggtaaggcc gatagtgcga tcacccgggc gctgagcgat
     4261 ccctccgacc aagtccgcgc ggctgccatg cagtcgatcg cggtcctcgc cagacggcgt
     4321 ggctcggcct cccccgagct ggtcggcgcc ctgacgaaga cgctcggctc ggcgtcgtgg
     4381 agcgaccgtc gggtcgccgc gctatcgctc ggcaagctgg gcagcgctgg tgatccgcag
     4441 gcactggcca aggcggccaa ggactcgtcc agcttcgtcc gcgaggccgt ggcggtcgct
     4501 ctcggcgaga tcggcggccc gcaggtggtc gagacccttc agatgctcgc caaagacgag
     4561 gtcgtgcagg tccgcgaggc ggccacgcat gcgctctcga cgctaaaacg ctagcgctac
     4621 ccgaagagac agcgccccgt tctttgacgc accgtttcgc aaaggggtag aatcacacgc
     4681 gatggacgtc ggcttggtgt gcgacgcgtg tagcgcgttg actccaatcg gagtgcccca
     4741 atgtgcgcgt tgcgggcaac cggtggccct cgacccgcgt ccgcggcgca cgagcaaacc
     4801 atccggaccc aatggcggcg cgactgcagc tgcagctgcg caaggtgccg gcacgccgtg
     4861 cggcaagtgc ggcacgacgg tgccgtcgaa cttcaagttc tgccagatct gcggcacccg
     4921 cgtcgttgtc gcgcaggact tccaggtcga gacacgcgtc ggtccgcgtc agtcgcaaac
     4981 cgccgacgcg caaaagcctg gccgcagcac gctgttcttc ggcggcgctg cgcaagctgc
     5041 gcgcgcaaag ctcacgctga tccgtggtga cggcgaagat ggcgtctcgt tcacgctcgc
     5101 cggaactgat cacctcgccg gtcgcggtga gtgtccgatc tcgttccccg atgatccgta
     5161 tctctcgccg attcactgca actttcgcta tgcgaagaac acccttgtcg ttcacgacga
     5221 gggctcgctc aacggcgtgt tcgtgcggat cagcggctcg gtgaaaatcg agcccggcag
     5281 tacggtgctc gtcggcgaac aggtgctcac ggtgtcaccg gcacgtggct tcgacgacct
     5341 gcccgatgct gagggaacgt attactcggc gagcatgatg cgtccggcga cgatcgagat
     5401 cgtgcagcag ttgcgcggtg gatcgtgcgg ctgggtgttt cggccagaca ccgagatggt
     5461 ctcgatcggc cgcgaaggca acgacatcaa cttccccgac gacccattca tctcgggcaa
     5521 gcatgcacag ctgcaactga ccggcggcgt gttaacagtt acggatctgg ggtcgcgcaa
     5581 cggtacgttc gtacgggtca ccggcgagca cgtgctcaag cacggcgatt acgtatttct
     5641 gggtcagcag ctactgcgtg tggaaatcgt ctagggacga tctcgtttac ggccaaagga
     5701 ccacccatga ccgtctgcaa tcgatgcgga aaagaaaatc aggaccacta taagttctgc
     5761 ctcgggtgcg gcgcagagct gacggcgccc aagccaggcg gcggcggcga catgggaatg
     5821 atgaagacca tgatggccga tccgggcgca ggcgccggtg caccgctcgg tggtccgcgc
     5881 ggtccaagtg gcgcgatgcc cggccagccg atgcgtccga gtggcggtgg tatgccgggc
     5941 atgccgccgt cgggtccgct cggtggcgcg ccgatggcgc ctccgccgat ggcaccgcca
     6001 atgggtcctc cgccgggcgt tccgtcgtac ggcaacttcc cgccaccggc tgcagcgccc
     6061 gccggcggaa tgcgtccgtg cccgtcgtgc ggcagcggtg tgccgccgag cttccgcttc
     6121 tgcggcacgt gcggcttccg catggacgag cagtccgcgc ccgcgccgat gccgcaaccg
     6181 ccgatgggcg gtgggttcgc gccgcagccg cagcgctcgc gcctgtcgat gacgctgatc
     6241 cgtcccgacg gcaccgaagg cggcacccat gatcttcgcc cgggcgagaa caagctcggc
     6301 cgcagcttcg gtcccgtctt cgagaacgac ggctatctgt cgccggtgca cgcgctgctc
     6361 gacatccgcg gcgtgcaagc agtcgtgcgc gacctcgact cgctcaacgg cgtgttcgtc
     6421 aagatgacgc aggaagagga gctgcagtcg gggcagatca tccgcatcgg ccaagagctg
     6481 ttgcggttcg agatcattcc gacgccagag ccgactgccg acggcaccga gctcatgggc
     6541 agcccgaacc cggggttctg gggcaagctg accgtgatca tcggtcgcga cgtcaccggc
     6601 gctgcgttcc cactgctcgg cgagagcgtc acgctcggtc gcgagcgcgg cgagatcaac
     6661 ttccccgacg acggctacgt gtcaggtctg cacgcgcgga tctcgctgcg tgaaggtcgc
     6721 gtgttcctcg cggacctcgg ctcgagcaac ggcacgttca tcaaggtgaa tggcgagcgc
     6781 aacgtcggcc acgagtcgtt cgtgctgctc ggtcagcagc tgttccgcct caacttctcg
     6841 taagcaggag gcggcgcgaa agcgcgagcg gcgcgcgacg tgcgcgcgtc gctacttctt
     6901 gatcttgctc gtatcgaggt ctttcacgtc catcgtcgac tcttgcttct cgacgaacgt
     6961 gaagcccttg gcaatcttca actcgccacc cggctcgaag atgatgagca cgtcaacctg
     7021 ctcgttcggc ttgccgcccg gcgcttcgac gatgagctcg ctgtcgctcg cgaagcgaac
     7081 gacggtgccc tggcgcgagc cgaagtacac cttcgcgttg cgggcgccgt cggcgatgaa
     7141 ccgattgccc ttgatgcgaa cataggtgcc gccgttgacg tcgcccttct cgggctcgag
     7201 gccggtgacc ttgagcttgg tgtcctcgct gccgcacgca gctaacacga tcacgagtag
     7261 aagcggcaag acagggaggc gcctggtcat gaccgaaacg tacctaactt ccgaggggat
     7321 gagcaactcc cgacggaact ggatggggca tcacgtgcca atggctagag caagtcgcgg
     7381 cgagccagcg cacggcgcat cttggccagc gcctgctcct ggagctggcg gatgcgctcg
     7441 cgcgacagat tgtacttgtc gccgatttcc ttgagggtca gctcctgctc ggtaccgagg
     7501 ccgaagcgct ggcggaggat gtctgcctcg atagggcgaa gctcgcggag caactcgcgt
     7561 acttcggtcg tgagcgcttc ccactcgaga tcctcggttg gggacacggc ctcgcgatcc
     7621 ggatcctcga gcacctcgcc gagcacgcgg ccctcgtcgt cgccgaccgg cttgtcgatc
     7681 gataccgact gctcgagcag ccagctccgc atcttctcga ccttgtcgag cggcatgttc
     7741 gtggcctctg cgagctcttc ggtggtcggc tggcggccga gcttgccggt gagctggcgg
     7801 cgcgccttgg tgagccggtg ctgcgcgtcg atcatgtgca cgggcaagcg gacctcgcgg
     7861 cccttgtcgg cgagcgcgcg gctgatcgcg tggcggatcc accagctcgc gtacgtcgag
     7921 aacctgaagc cgcggcggta gtcgtagcgc tcgaccgcct tcatcaggcc gatgttgccc
     7981 tcctggatga gatccgcgag ggccatgcgg ccgtggttga agcgccgcgc gatgctgacg
     8041 acgaggcgca ggttcgcttt gacgaagtcg ttacgcgcat ccgcggccgc acgatttgcc
     8101 tgcgagacgc ggcggagcca ctcagcaact ttcgtacgct ggccgagcgc accgactgcg
     8161 gagccacgca cgccgatgcc gagcgtcatc cgatcgatcg cgccgagcgc cgcttcgatg
     8221 aacaggtggt cgacatcgcc cgagcgcagc ttgcgcgccg cacgtgccga cgctcgcgca
     8281 aacgctttgg cgtcgcgctc gctcgccatc cggcgcagcg tcttcgcttc gagcggcatc
     8341 acgaaatcag gaactgtcgc aacggcctcg aggatgcgct cgatcgcctg cgggtgagag
     8401 agcaccgctt cccacaacat gatctcgagc gcttcgattt cgcgtgcgct cgtgaactct
     8461 tcttcgggac ggagcacgtc gagctccgcc atgtcgcgga agtacatcga caggtacgtg
     8521 ccgttatcgg ggcgtgcagg tttctctgcg gcctcgactt cttcttcgcc gtcgagctcg
     8581 acgtcggatt cgggctcctc gtgcttggga cgcttggccg tgacgcgcgc gacgcgagac
     8641 ttacgggcgg tctgctgcag tgcctgcgga agcttggaga caatagcggc catttcggtc
     8701 cttccctgcg cgcgggcgct tactcactga gacgcgcgag gggaccaaat gttttggcca
     8761 tcttcgcggc acggagggac aacagccggg agccggactt gattccggct taagtccctg
     8821 aagtttcctt ccgttccgct aagacgatga tgtgacgcga ctggttgccg aaaaaacggc
     8881 cgcgcatctg ataaccgccg gatacttcga gaactcggaa accggcggcg tgcatcagct
     8941 taccaacctc atgcagtgag tatgcccgga ccgagatctc ctgctcgagc tggcgaccgt
     9001 cgtcgaacac gacggaacga ttcacctgaa tgcgcgacga gaagtagttg agctcgactt
     9061 cttcgagcac gacgcagcca tcgccctccc accacacacg tgtcggcaag tcagcgatca
     9121 cgtagtcgcg gttgagaatc tcgacgagca ccttgccatt gggcttcagc gcgcgtgcca
     9181 cattttgcag cgtcttcttg ttcgtctcgt cgtcgaagta gccgaacgtc gagaacaggc
     9241 agtagacgcc gtcgaactgg ttctcgaagt cgagctcgcg catgtcgccg cgcacgaagt
     9301 tgatcgtgag cccacggcga tgcgcctctt caccaccgcg cacgagcagc ggcgtcgata
     9361 gatccaaacc gacaacgtgg aagccacgcg ccgcgagctc catcgcgtga cggccgtagc
     9421 cgcaaccgag gtcgagcacc tgcgcacccg gcgcgaggtt catcgcgttg atcacgaact
     9481 ccgcttccgc ctgcgtcgcc tgcggcgtca agaacggcag cgtgcgcaga tagtcttcgt
     9541 cgaatagatc gacgaaccac ggcttcgcac gcccgctcgt cggcttgctc ggctccttcg
     9601 ccaccacggc ggcgacggct gcttgcggga tgggcttctt cgccggcggc ggtgcgggcg
     9661 gcggtttgtg cgcggcggcc ggcggcgtcg gcggcggatg atgttccttg ctgacctgcg
     9721 cggtcggcgc gggcggcgga tgctgcgccg gcggcgaatg ctgcgcttcg acgatcgctc
     9781 cgctcttgcg gtcgatggtc ggcgccgact ggttgcggat ctgcggcgga accgacggcg
     9841 tcgacacgcg tgtgctcggc ggcggctcgg caaccttctc gatgaccttc tcgtcggcct
     9901 tgtcctcgtc gacgtcgacc tcgatctcgc ccgacgactc ggcgcggtcc gcgagctcct
     9961 ggatgtcgat atcggggacc gacatcggct cttcgcttgc cggtgacttc gtgagcactt
    10021 cgacgtcgat cgccgcgagc ggcatcgtgc gattcgagtc ggcggctttc ggtttcgccg
    10081 ccaccggctg cgtcgccgcg cgcacttctt cgagatcctc ctcggtgagc gcgacggtct
    10141 tgtggcggct tcccggaagc ttcttcgcac cgccggtggt cggcgcttga atctgccgtg
    10201 tttgcttcga gaacggatct tcgatggcgg gacgctgcgc ggtgaccggc tcgcgatccg
    10261 gatccggcgt ttcggtatcg agcgtgacgt cgacgtcgac atcgacggta cgctcaccac
    10321 gcatcgccgg catgccaggc gacgacagct ggctgtacgc cgacgacacc gtgtcggagg
    10381 tcgagagccc gagcgcctcg agatcgaccg cgctcatctc gcgcgtcttc atctcgaagt
    10441 cctcggacgc ctgcgggttc ggcggatcga tcactgtcgg cacggcatcg ggcgcgatcg
    10501 gggcgggtgc ggaaatttcg ttgtggaccg cgcgcggca
//
LOCUS       DASUFX010000545        13582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1476761, whole genome shotgun sequence.
ACCESSION   DASUFX010000545 DASUFX010000000
VERSION     DASUFX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13582
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476761"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFV99_21725"
     CDS             complement(<1..421)
                     /locus_tag="VFV99_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5062010.1"
                     /translation="MPARYLIGIDLGTTNSAVAYVDTKAADPRVRVFEVPQLVAPGEV
                     APRRQLPSFVYLASDIDLAPHETALPWRADAPTTALRAIVGELARNQGARMASRMIAS
                     AKSWLCHPGVDRGAAILPWGEHDGPKLSPINAQAQVLR"
     gene            550..2793
                     /locus_tag="VFV99_21730"
     CDS             550..2793
                     /locus_tag="VFV99_21730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HEX5062011.1"
                     /translation="MRLCLIVCLLAACEGPAGPPGGDGTDGTNGSNGAPGDTGPKGDP
                     GTTPVGPWVVADKVDVSVTGLTFDASGAHVAFTLKDKDGKPLDRTGNLTEGKVTVSFV
                     LAQLATASDGTAAQYTAYTKRTVAAVPPYPTNSVTQATTEGTEANFETVDVAEGSYRY
                     NFMAPVTDYDSTKTQSVLAVAARTVDNVQSYDRDLFHVGTVRRDEVGDSTCGSCHGSF
                     AAHGGRYTKAEQCVLCHTPQSADPETNNTVDFKVMIHKIHRGEDLPSFKADATNKYTI
                     VGFGPTVHDFSEVAFPGPTTSLAQNISRCETCHTGTQATNWWARPSKAACTSCHDTTV
                     FTASPAAPLVEHQGGVDPTLVNDSTCIVCHSMTAGPAPVPAAHYTGIWDPTTGTMNVT
                     IQTITNTAPGQLPTVRFKVMVNGAPRDILAAPLSALTATVAGPTTDFREFWQARMQGT
                     GAVGTLAAVDAANGIFDYTFPSTTNPASPPCTESTIKRDCQIPATATGSYQIAFEANW
                     TPTGGARIVAPTPRLAFAVTDATATPRRTIVDNTRCNGCHYDLQFHGGGRKDVQYCVM
                     CHNPNNANDERISRLEGSTVFAESVDLRVMVHKIHMGEELTQSYILGGNPAPTVTNPL
                     GAPTDFGKVRYPRDRTACAACHTGTTWTLPMTASANYLPSTTVELTCSEPTGNDTDNY
                     CTAPFWNITKTTKIAPESSVCTSCHDAPYTAAHAVLNTTSGGVEACTTCHGPGKEFDV
                     ARYHGTP"
     gene            2790..3905
                     /locus_tag="VFV99_21735"
     CDS             2790..3905
                     /locus_tag="VFV99_21735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX5062012.1"
                     /translation="MRFAVVMLALASCSEERVLSQPCDGYECVVTVHERGIQDEDSAD
                     FHGKLLARTNWDFAKCASCHGADFAGGTSGVSCLSCHKQGPTACATCHREERESGSHK
                     LHRSAALTCAECHVVPASWDAPGHIVDDSAPAEVTFGALAATTLDPADRAGPPTFDGS
                     RCSNVYCHGAVMPGGGTLTQPVWNATATGACGSCHAAPPPSHARTDCATCHPSGAPHV
                     DGIVNVGKTNDCSGCHGSASSPAPPADLAGNMFTTAIGVGAHQAHLQAPSGLRGPIPC
                     ETCHAVPATVTAVGHLDPAPAEVNAELGWNRTTQTCTTWCHGVARPAWTSHGEVVCGS
                     CHGAPPSDANHNAQMTVTTCVNCHAAGKHMNGVVDGL"
     gene            3895..4560
                     /locus_tag="VFV99_21740"
     CDS             3895..4560
                     /locus_tag="VFV99_21740"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX5062013.1"
                     /translation="MGFNPLAWITIGFAASSAVLVIWFLLRRPLLTRSTKIILLFGIG
                     LLPLGTAMTGNYAGFEATKQRSFCGSCHVMTPYANDSGDPKSETLAARHGRNGMFGDE
                     NCYTCHADYGMFGTVKTKLGGLRHVYEYVLHYHDMPLEEAKRTIHIRQPFKNETCMHC
                     HSTQNPGWNAVAEHKSILEPVRTGAVSCASEGCHGPAHPFTKNLSANDPSTKDLTVKG
                     HTP"
     gene            4557..5228
                     /locus_tag="VFV99_21745"
     CDS             4557..5228
                     /locus_tag="VFV99_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062014.1"
                     /translation="MNLRIRHVSIWLRVSAVLTLIALALMVWSVLVPTPLPVMLAMTV
                     GQGLGTAAFGIFGLVVLKDLTRSRRAKRDSMANIALQTIALTKDVLARDSLQNIALSD
                     DDASRDSEKNIALTRKTTRDSLQNIALSKDDAPRDSQRNIALAKDDAPAGRDSQRNIA
                     TAKDDAPAPRDSQQTIAPAKDDAPAPRDSQQTIAPAKDDAPAPRDSQRDIAPAKDDAP
                     AKDES"
     gene            5225..6406
                     /locus_tag="VFV99_21750"
     CDS             5225..6406
                     /locus_tag="VFV99_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062015.1"
                     /translation="MTRASAIQLVLAAVLMHAASAARADPLRLRADALGGTQSPAGLI
                     VLEADGKASATTNAEAIVWAANDSADVLVIALRARTADGRIGGKVGRFVASLGALRPV
                     QIDGASGRMRLPHSFDVEAIGGVPVPPAPSFGHSISERAWDWVAGGRIARRFGDYGSV
                     GIAMLEQRDDARLATREVGVDAGIALTKRHDVATRVAYDLANPGLADAYASLIRRGGG
                     VRAEVYAGYRAASHLLPATSLFTVLGDTPSSRGGMQISGKLAPRLELGSDMGLRGAAD
                     ELGVLLVLRARLALDDKFASAITGELRRDGVGEDAWTGARGAARIALSHSLTASTELE
                     LVVPDRDRMRGAVWPWALAALAYQRGPWQAAAAIEASASAQYAHRVDVLVSMMRMWGQ
                     P"
     gene            6391..7431
                     /locus_tag="VFV99_21755"
     CDS             6391..7431
                     /locus_tag="VFV99_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062016.1"
                     /translation="MGAAVKRLALAILVLGIAACAGVLGLRAREPTAFPHRKHVLNGV
                     ACTRCHVGIDKANTNDGVLHLPDEASCIGCHAKPHDEPPCFGCHSRPTARAELLEARD
                     HLRFDHARHLGKVQGNCMRCHVGVGEGDERLRPTMATCFKCHDGERDARQCDSCHKNL
                     EAGTEKPASHLAHDGDWLRDHGARAASSGDVCSTCHSERSCAKCHGVTVAALPAKQQF
                     ANPFTPSVHRAGFTSRHSLEAKADPGACQTCHQPDRCVSCHVSKGVAGVDRASPHPPG
                     WVGVTENQHGREARRDPAACASCHGGAGEQLCVQCHKVGGVGGNPHPAGWSSKLPLSA
                     MPCRMCHPLGAR"
     gene            7446..9080
                     /locus_tag="VFV99_21760"
     CDS             7446..9080
                     /locus_tag="VFV99_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062017.1"
                     /translation="MKRWCAMKRWLVLVGCALAVAGASFVACGFADRPHAAEHVPVAW
                     REVRFAIGHQKHVGKVACADCHGAGFAKPAADLCTNCHAKTSSLHHDDPTTPDGAPRC
                     TDCHGYSADTSITPWNCMRCHQDDRGHVKAIGAHADEDCKTCHDPHGNPATTPRDCAS
                     CHTGRETKHAGLHGCLDCHSMHEAKLPTLSERTGTSAAAGCERCHAKQPGKLHVDARA
                     LSTGHDACTGCHKPHDFTPQPCINCHQDTQMIAPAKHKCMGCHDQHDTSAPKQCASCH
                     TQQVKHPVTPQGACIGCHRPHQPQAACESCHQTVHKHAATTNCLDCHTPHAGKPQPNA
                     ALCGKCHTANAQKAAGHSECITCHKTAAHTPSKQTPACASCHADKARDAGKHAQCASC
                     HTTIHAPATQPPACATCHKPEATTAPTGHAQCASCHQPHAPTKIPACASCHTSEAKTK
                     HGNIACATCHRPHGPGGVTTAPACATCHVARTGPAPGPQPVKLHASKGHADCMTCHVS
                     HEARPKDDRASCLACHKAQVNHEPTAAHCATCHPFR"
     gene            9668..10324
                     /locus_tag="VFV99_21765"
     CDS             9668..10324
                     /locus_tag="VFV99_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062018.1"
                     /translation="MRGVLVVLLLVVQPVAAQPAAAPPMAAPPPATPSTCDPAEAASL
                     RAHLEHEQHRAKRWNVAWGAIFGGLSVASFAVAIADPFPDQTPGFYVSGSKSAIGALS
                     RVITPLRVPVPPADADVCTDIANLHKAVKTAARHERGNFYLNHFGAIFLNGVGGMILW
                     HYSDGKKALINAVTGYPVGLLSVYTAPRGSWHLYRDATWSVGVVPQQGAWLLTVSGEL
                     "
     gene            10481..11104
                     /locus_tag="VFV99_21770"
     CDS             10481..11104
                     /locus_tag="VFV99_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062019.1"
                     /translation="MRWALVVLLLLAQPVRAHAACDPTEAETLRAHLERQSDRARNWN
                     IIWGAVFGTAAIGTFTVAVTDAIPDLTPGLYASSGKATIGALGRLILPLRIPVPPVDA
                     DTCTEIANLHKAIKEAAKREKGNFYVNHAGGIIVNGVAAAILWKYDTGAHALLSVATG
                     YPVGVLSNYTAPRGSWHMYRDADWAITVTPPQSASNSPWMLTLGGGF"
     gene            11131..11685
                     /locus_tag="VFV99_21775"
     CDS             11131..11685
                     /locus_tag="VFV99_21775"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5062020.1"
                     /translation="MAYRDRWRAESAELQRILSGFDLRVERKWGKPCYTLNGKNVVLI
                     QGFKESCALLFFKGALLKDPKKLLVQLGRVHAARVMKFASAEEIARKAATIKAYVRQA
                     VAAEKAGLKVETKPREFPVPAELEEKFRKNPRFKRAFEALTPGRQRGYLFHFGGAKQS
                     ETRTARIEKAMPAIFEGRGFLERR"
     gene            complement(11846..12664)
                     /locus_tag="VFV99_21780"
     CDS             complement(11846..12664)
                     /locus_tag="VFV99_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062021.1"
                     /translation="MLDPVVGASKLALDKEHLIVSGTLDCPRLFVMHRDGTGRHVVFD
                     ANSLSNVCDVWADDRDIFVSCDRGLGRVDKSGTFRQLAPYTHAHICCDEEFLYGTQGV
                     GRDENEIGAFRIPRTGGELEWLVRGPTSALTVHDGRLYYYQHQLIRVREPDGATRALA
                     QAEWPGSIVVLGPDEIAWTQNGLVASAKDRLPRTLATPRIATGMIRRGDTLYWGAWEP
                     NKQGLWYMRIDDPTPALVAKFPHKSLSIVADDTHLFWTGHVNGGVYALPRSSRG"
     gene            complement(12704..13147)
                     /locus_tag="VFV99_21785"
     CDS             complement(12704..13147)
                     /locus_tag="VFV99_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062022.1"
                     /translation="MASALDTIAHLIVPNVLKLKGAAMLIGAMERRDASAFAQVWQQT
                     GVEHTPQVSAKEKGDWRFGVMSLPKPSAMGEAYMCAFVTKKNDGSVNRYFTLEYDYVL
                     ATKTEKTILCEREGSLTKKRGDGSPITGDFQADASAFVDAIADKL"
     gene            complement(13238..>13582)
                     /locus_tag="VFV99_21790"
     CDS             complement(13238..>13582)
                     /locus_tag="VFV99_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5062023.1"
                     /translation="LCVAPQGLEEGSTVELPDDELGLVVGETSTFRFFAAATRKDDGA
                     GTLVDADVEGIVELDPVEKAVAADGDRKSGDMVPVKLEAHVTEVGTLELWCVARDGNG
                     RWKLEYSVRERE"
BASE COUNT         2351 a         4521 c         4616 g         2094 t
ORIGIN      
        1 gacggagcac ctgcgcctgc gcattgatcg gcgacagctt cggtccgtcg tgttcgcccc
       61 acggtaagat ggccgctcct cgatccacgc cgggatgaca cagccacgat tttgcagagg
      121 cgatcatgcg cgacgccatg cgcgcgcctt gattgcgtgc gagctcgccg acgatcgcgc
      181 gcagtgcagt cgtcggtgca tcggctcgcc agggcaacgc ggtctcgtgc ggagcgagat
      241 cgatgtcgct cgcgagatag acgaacgacg gcagctggcg gcgcggcgcg acttcgccgg
      301 gcgcgacgag ttgcggcacc tcgaacacgc gcacgcgcgg gtcggccgcc ttggtatcga
      361 cataggcgac cgcggaattg gtcgttccga ggtcgatgcc gatcaggtag cgcgcgggca
      421 cgcgccgctt cttaccatgc ggggtccgcg caaaacctgc agggggccga aaccgagttg
      481 cagttcttgc ggggaacccg cccaaaactg gcgacttccg cgggcatggc tcttgagtaa
      541 tgggacggta tgcggctttg tctgatcgtc tgtcttctgg cggcgtgcga aggacctgcg
      601 gggcctccgg gtggtgacgg caccgatgga accaacggtt ccaacggcgc cccgggtgac
      661 accgggccca agggcgaccc cggcacgacg ccagtcggcc cttgggttgt cgcggacaag
      721 gtcgatgttt cggtcaccgg cctgacgttc gacgcgagcg gcgcgcatgt cgcgttcacg
      781 ctcaaggaca aggatggcaa gccactcgat cgcaccggca acctcaccga aggcaaggtg
      841 acggtcagct tcgtgctcgc gcagctcgcg accgcgagtg acggcaccgc cgcgcagtac
      901 accgcctaca cgaagcgcac cgtcgcagcc gtgccgccgt atccgacgaa cagcgtgacg
      961 caagcgacga ccgaaggcac cgaagcaaac ttcgagaccg tcgatgtcgc ggagggcagc
     1021 tatcgctaca acttcatggc gccggtcacc gactacgatt cgacgaagac gcagagcgtg
     1081 ctagcggtcg cggcgcgcac ggtcgacaac gtgcagtcgt acgatcgcga tctgtttcac
     1141 gtcggcaccg tgcgtcgcga cgaggtcggc gattcgacgt gcggcagctg ccacggctcg
     1201 ttcgccgcac acggtggtcg ttacacgaag gccgagcagt gcgtgctctg ccatacgccg
     1261 cagagcgcgg acccggagac gaacaacacg gtcgacttca aggtgatgat ccacaagatc
     1321 caccgcggcg aggatctgcc gagcttcaag gcggacgcga cgaacaagta cacgatcgtc
     1381 ggcttcggtc cgacggtgca cgacttctcg gaggtcgcgt tccccggtcc gacgacgagc
     1441 ctcgcgcaga acatctcgcg ctgcgagacc tgccacaccg gtacgcaggc gacgaactgg
     1501 tgggcgcggc cgagcaaggc ggcgtgcacg tcgtgccacg acacgaccgt gttcaccgcg
     1561 agtccggcgg cgccgctcgt cgagcaccag ggcggtgtcg atccgacgct cgtcaatgac
     1621 tcgacgtgca tcgtctgcca cagcatgacc gccggcccgg cgccggtgcc ggccgcgcac
     1681 tacaccggta tctgggatcc gacgaccggc acgatgaacg tgacgatcca aacgatcacg
     1741 aacaccgcgc ctggccagct gccgaccgtg cggttcaagg tgatggtcaa tggcgcgcca
     1801 cgcgacatcc tcgccgcgcc gctgtcggca ctgaccgcaa cggttgcggg cccgacgacc
     1861 gacttccgcg agttctggca agcgcgcatg cagggcacgg gtgcggtcgg tacgctcgcc
     1921 gcggtcgacg ccgcgaacgg catcttcgac tacacgttcc cgtcgacgac gaacccggca
     1981 tctccgccgt gcaccgagtc gacgatcaag cgcgactgcc agatcccggc gaccgcgacg
     2041 ggcagctacc agatcgcatt cgaggcgaac tggacgccga ccggtggtgc tcgcatcgtc
     2101 gcgccgacgc cgcgcctcgc gttcgcggtg accgatgcga ccgcgacacc gcgccgcacg
     2161 atcgtcgaca acacgcgctg caacggctgc cactatgatc tgcagttcca cggtggtggg
     2221 cgcaaagacg tgcagtactg cgtgatgtgc cacaacccga acaacgcaaa cgacgagcgc
     2281 atctcgcgcc tcgaaggctc gacggtgttc gccgagagcg tcgaccttcg cgtgatggtc
     2341 cacaagatcc acatgggcga ggagctgacg cagtcgtaca tcctcggcgg caacccggcg
     2401 ccgacggtga cgaacccgct cggcgcgccg acggacttcg gcaaggtgcg ctacccgcgt
     2461 gaccgcaccg cgtgcgcggc gtgccacacc ggtacgacgt ggacgctgcc gatgacggcg
     2521 tccgcgaatt acctgccgag cacgaccgtc gagctcacgt gcagcgagcc gaccggcaac
     2581 gacaccgaca actactgcac cgcgccgttc tggaacatca cgaagacgac caagatcgcg
     2641 cccgaaagct cggtgtgcac gagctgccac gacgcgccgt acacggccgc gcacgctgtg
     2701 ctcaacacga cctcgggtgg tgtcgaagcc tgcacgacgt gccacggccc gggcaaagag
     2761 ttcgacgtcg cgcgctacca cggcacaccg tgaggttcgc ggtcgtcatg ctcgcgctcg
     2821 cgtcgtgttc cgaggagcgc gtgctgtcgc aaccgtgcga tggctacgag tgcgtcgtga
     2881 cggtccacga gcgcggcatt caggacgagg acagtgctga ctttcacggc aagctgctcg
     2941 cgcggacgaa ctgggatttc gcgaagtgcg cgagctgtca cggtgcggac tttgcgggcg
     3001 gtacgtcggg cgtgtcgtgc ctcagctgtc acaagcaagg gcctaccgcg tgcgcgacgt
     3061 gtcaccgcga agagcgcgag agcggctcgc acaagctgca tcgcagcgcg gcgctgacct
     3121 gcgcggagtg tcacgtcgtg ccggcgagct gggacgcgcc tgggcacatc gtcgacgaca
     3181 gcgcgcccgc ggaggtgacg tttggtgcgc tcgcggcgac gacgctcgat ccggcagatc
     3241 gcgcgggccc gcctacgttc gatggcagca ggtgctcgaa cgtctactgc cacggcgcgg
     3301 tgatgccggg cggcggcacg ctgacgcaac cggtgtggaa cgcaacggcg acgggcgcgt
     3361 gcggtagctg tcacgcggca ccgccgccga gccatgcgcg caccgactgc gcgacgtgcc
     3421 atccatcggg tgcgccgcat gtcgacggca tcgtcaacgt cggcaagacg aacgattgct
     3481 cgggctgtca tggcagcgcg agctcgccag cgccgccagc ggatctcgcg ggcaacatgt
     3541 tcacgacggc gatcggcgtc ggtgcgcacc aagcgcatct gcaggcaccg tcgggattgc
     3601 gcgggccgat tccgtgcgag acgtgccacg cggtaccggc gacggtgacg gcggtcggcc
     3661 acctcgatcc ggcgccagcg gaggtcaacg cggagcttgg ctggaatcgc acgacgcaga
     3721 cgtgcacgac gtggtgtcac ggcgtcgcgc ggccggcatg gacctcgcac ggcgaagtgg
     3781 tgtgcggctc gtgtcacggc gcgccgccgt ccgacgcgaa ccacaacgca cagatgacgg
     3841 tgacgacgtg cgtgaactgt cacgcggcgg gcaagcacat gaacggagtt gtcgatgggc
     3901 tttaatccgc tcgcctggat cacgatcggc tttgccgcga gctcggccgt gctcgtgatc
     3961 tggttcttgc tgcgccggcc gctgctcacg cgctcgacga agatcatcct gctgttcggc
     4021 attggcctcc tgccgctcgg caccgcgatg accggcaact acgccggctt cgaggcgacg
     4081 aagcagcgct cgttctgcgg cagctgtcac gtgatgacgc cgtacgcgaa cgactccggc
     4141 gatccgaaga gcgagacgct cgcggcgcgc cacggccgca acggcatgtt tggcgacgag
     4201 aactgctaca cgtgccacgc cgactacgga atgttcggca cggtgaagac gaagctcggc
     4261 gggctgcgcc acgtctacga gtacgtcctc cactaccacg acatgccgct cgaggaagcg
     4321 aagcgcacga tccacatccg gcagccgttc aagaacgaga cgtgcatgca ctgccactcg
     4381 acgcagaacc ccgggtggaa tgccgtcgct gagcacaaga gcatcctcga gcccgtgcgc
     4441 accggtgcgg tgagctgtgc gagcgagggc tgccacgggc cggcgcatcc gttcacgaag
     4501 aacctctccg ccaacgatcc gtccacgaag gatctgaccg tgaaggggca cacgccatga
     4561 acctgcggat ccgtcacgtc agcatctggc tgcgggtctc cgcggtgctc acgctgatcg
     4621 cgctcgcgct catggtgtgg tcggtactcg taccgacgcc gctgccggtc atgctcgcga
     4681 tgaccgtggg gcagggcctc ggcaccgccg cgttcggcat cttcggcctc gtcgtgctga
     4741 aggacctgac gcgctcccgg cgggcgaagc gcgattcgat ggcgaacatc gcgctgcaga
     4801 ccatcgcgct gacgaaggac gtgctcgcgc gcgactcgct gcagaacatc gcgctctcgg
     4861 acgacgacgc ttcgcgcgac tccgaaaaga acatcgcact gacgaggaaa acgacgcgcg
     4921 attcgctaca gaacatcgcg ttgtcgaagg acgacgctcc gcgtgactcg cagcggaaca
     4981 tcgcgctcgc gaaggacgac gcgccggcgg gacgcgactc gcagaggaac atcgcgaccg
     5041 cgaaggacga cgcgccagcg ccgcgcgatt cgcagcagac catcgcgccc gctaaggacg
     5101 acgcgccagc accgcgcgat tcgcagcaga ccatcgcgcc cgcgaaggac gacgcgccag
     5161 caccgcgcga ttcgcagagg gacatcgcgc ccgcgaagga cgacgcgcca gcgaaggatg
     5221 aatcatgacg cgggcgagtg cgatccagct cgtgctcgcg gccgtgctca tgcacgcagc
     5281 ctcggcagcg cgcgcggacc ccttgcgctt gcgcgccgat gcgctcggcg gcacgcaatc
     5341 accggcaggc ttgatcgtgc tcgaagccga cggcaaggcg agcgcgacga cgaacgccga
     5401 ggcgatcgtg tgggccgcga acgacagcgc cgacgtgctg gtgatcgcgc tgcgcgcgcg
     5461 cacagcggat ggccgcattg gcggcaaggt cggccggttc gtcgcatcgc tcggcgcgct
     5521 gcgcccggtg cagatcgacg gtgcgtcagg gcgcatgcgg ctgccgcaca gcttcgatgt
     5581 cgaggccatc ggcggcgtcc cggtgccgcc ggcgccgtcg ttcgggcaca gcatcagcga
     5641 gcgcgcgtgg gactgggtcg ccggcggtcg gatcgcgcgc cggttcggcg actacggctc
     5701 ggtcggcatc gcgatgctcg agcaacgcga tgatgcgcga ctcgctacgc gtgaagtcgg
     5761 tgtcgacgcg ggcatcgcgc tcaccaaacg tcacgacgtc gcgacgcgcg tcgcgtacga
     5821 cctcgcgaat cccggtctcg ccgatgcgta cgcgagtctg atccggcgcg gcggcggtgt
     5881 gcgtgccgaa gtctacgccg gctatcgcgc ggcatcgcat ctgttgcccg cgacgtcgct
     5941 gttcacggtg ctcggcgaca cgccatcatc gcgtggcggc atgcagatca gcggcaagct
     6001 cgcgccgcgg ctcgagctcg gtagcgacat gggcttgcgc ggcgcggccg acgagctcgg
     6061 cgtgttactc gtgctgcgcg cgcgcctcgc gctcgacgac aagttcgcga gtgcgatcac
     6121 tggcgagctg cggcgcgatg gcgtcggcga ggatgcatgg actggcgcgc gcggtgcggc
     6181 gcggatcgcg ttgtcgcatt cgctgactgc gagcacggag ctcgagcttg tcgtgcccga
     6241 tcgcgatcgg atgcgcggcg ccgtgtggcc gtgggcgctc gctgcgctcg cgtatcagcg
     6301 cgggccctgg caagcggccg ctgcgatcga ggcgtcggcg tcggcgcagt acgcgcatcg
     6361 cgtcgacgtg ctcgtgtcga tgatgcggat gtgggggcag ccgtgaagcg cctcgcgctc
     6421 gcgatcctcg tgctcggcat cgctgcatgc gctggcgtgc tcggcctgcg cgcgcgcgag
     6481 cccactgcgt tcccgcatcg caagcacgtg ctgaacggcg tcgcgtgcac gcgctgtcac
     6541 gtcggcatcg acaaggcgaa cacgaacgac ggcgtgctgc acttgcccga cgaggcgagc
     6601 tgcatcggct gtcacgccaa gccgcacgac gagccgccgt gctttggttg ccactcgcga
     6661 ccgaccgcgc gtgccgagct gctcgaagcg cgcgaccacc tgcgtttcga tcacgcgcgc
     6721 caccttggca aggtgcaggg caactgcatg cgctgtcacg tcggcgtcgg cgagggcgac
     6781 gaacgcctgc ggccgacgat ggcgacctgc ttcaagtgtc acgatggcga gcgcgacgcg
     6841 cgccagtgcg acagctgcca caagaacctc gaggcgggca ccgagaagcc ggcgagtcac
     6901 ctcgcgcacg atggcgattg gctgcgcgat cacggtgcgc gtgcggcgtc gtcgggcgac
     6961 gtctgctcga cctgtcacag cgaacgctcg tgtgcgaagt gccacggcgt gaccgtcgca
     7021 gcgctgccgg cgaagcaaca gttcgcgaat ccgttcacgc cgagcgtgca tcgtgccggc
     7081 ttcacgtcgc ggcacagcct cgaggcgaag gcggatccgg gtgcgtgtca gacctgccat
     7141 cagccggatc gctgcgtgtc gtgtcacgtg tcgaagggcg tcgccggtgt cgatcgcgcg
     7201 agcccgcatc cgccgggctg ggtcggcgtc accgagaacc agcacggtcg cgaggcgcgt
     7261 cgcgatccag ccgcgtgcgc gagctgtcac ggtggcgccg gcgagcagct gtgcgtgcag
     7321 tgtcacaagg tcggcggcgt cggcggcaat ccacatcctg cgggctggtc gagcaagctg
     7381 ccgctgtcgg cgatgccgtg ccggatgtgt catccgctag gtgcgcgatg aaggcgtggc
     7441 gcgcgatgaa gcgctggtgt gcgatgaagc gctggctcgt gctcgtcggt tgcgcgctcg
     7501 cggtggcggg tgcgagcttc gttgcgtgtg gcttcgcgga ccgtccgcac gcagcggagc
     7561 acgtgcccgt ggcgtggcgc gaggtgcggt tcgcgatcgg tcaccagaag catgtcggca
     7621 aggtcgcgtg cgccgattgc cacggcgctg ggttcgcgaa gcccgcggct gatctgtgca
     7681 cgaactgcca cgcgaagacg tcgtcgctgc atcacgacga tccgacgaca cccgacgggg
     7741 cgccgcgctg caccgactgc cacggctaca gcgccgacac gtcgatcacg ccgtggaact
     7801 gcatgcgctg tcatcaagat gatcgcggcc acgtcaaagc tatcggcgcg cacgccgacg
     7861 aggactgcaa gacctgccac gatccgcacg gcaatccggc gacgacaccg cgcgactgcg
     7921 cgagctgcca cacgggtcgc gagacgaagc acgcgggcct gcacggctgt ctcgattgcc
     7981 acagcatgca cgaggcgaag ctgccgacac tgtcggagcg caccggcaca tcggcggcgg
     8041 ccggctgcga acgctgccac gcgaagcaac cgggcaagct gcacgtcgac gcgcgcgcat
     8101 tgtcgaccgg ccacgatgcg tgcacgggct gtcacaagcc gcacgacttc acgccgcagc
     8161 cgtgcatcaa ctgtcaccag gacacgcaga tgatcgcgcc ggccaagcac aagtgcatgg
     8221 gctgtcacga tcagcacgac acgagcgcgc cgaagcaatg cgcgagctgc cacacccagc
     8281 aggtcaaaca tcccgtgacg ccgcaaggcg cgtgcatcgg ttgtcatcga ccgcatcagc
     8341 cgcaagccgc gtgcgagtcg tgtcaccaga ccgtgcacaa gcacgccgcg acgacgaact
     8401 gcctcgattg ccacacgccg catgcgggca agccgcagcc gaacgccgcg ctgtgcggca
     8461 agtgccacac cgcgaacgcg cagaaggcgg cgggccacag cgagtgcatc acgtgtcaca
     8521 agaccgccgc gcacacgccg agcaagcaga cgccggcctg cgcgagctgt cacgccgaca
     8581 aggcgcgcga tgcgggcaag cacgcgcaat gcgcatcgtg ccacacgacg atccacgcgc
     8641 cggcgacgca gccgcccgcg tgcgcgacgt gccacaagcc ggaagcgacg acggcgccga
     8701 ccggccatgc gcagtgcgcg agctgtcatc agccgcacgc gccgacgaag attcccgcgt
     8761 gcgcgagctg ccacacgagc gaagcgaaga ccaagcacgg caacatcgcg tgcgcgacct
     8821 gtcaccggcc acacggtcca ggcggtgtca cgaccgcgcc ggcatgcgcg acctgtcacg
     8881 tcgcgcgcac gggacctgcg cccggcccgc agccggtgaa gctgcacgcg tcgaagggtc
     8941 acgccgactg catgacctgc catgtctcac acgaagcgcg cccgaaggac gatcgagcga
     9001 gctgcctcgc ctgccacaag gcgcaggtca atcacgagcc gaccgcggcc cactgcgcca
     9061 cctgccatcc gttccgctga cgacgaccgt agtatgatcg tcgatcgatg aggatggccg
     9121 tcgttgtcag cctttgcgca gcgtgcgcga gcgactctgg gacacacacg gcgatgagtg
     9181 ggcctgcgac gctcgtcgta gataacgtgc cgagcgcaat gtcggccatc ttggttccgg
     9241 cggcgacgac agatgcgcag aaccgtccgg ggtattgggg ctggaccgtg gggttttatg
     9301 ccgcgccgcc cggcgcctgt gacggcatcg ttggggggaa aggtgagctc gaggtcgtca
     9361 cgagcatgtt ctccacctca gcctcggacg tcacgatccc agtgggcacg atatcgatca
     9421 cgaggaacct tccggtcccc atcacgagcc cggttgcgat cgtgtgggca gcgggggacg
     9481 tcaccgtcaa tctcgatgct ggctctgcca cgatctcggc gttcacgtcc gagtctatgg
     9541 acggcaactt cagcggtacc gggacggacg caacgacgaa tgcgatcgtt ccagtcacgg
     9601 ggacgttcag cgcgcgtcga tgtgaatgac gcccccgcgc gtaggtcgct cgtgatagca
     9661 acgagggatg cgcggggtgc tcgtcgtgtt gttactcgtc gtgcagcccg tagctgcgca
     9721 gccagcagca gcgccaccca tggctgcgcc gcccccggct acgccgagca cgtgtgatcc
     9781 ggcggaggcg gcgtcgctgc gtgcgcacct cgagcacgag caacaccgcg cgaagcggtg
     9841 gaacgtcgcg tggggcgcga tcttcggcgg gctttcggtc gcctcgtttg cggtcgcgat
     9901 cgccgatccg tttcccgacc agacgcccgg gttctatgtc agtggcagca agtcagcgat
     9961 cggcgcgctg tcccgcgtga tcacgccgct gcgcgttccg gtaccgcccg ccgacgccga
    10021 cgtctgcacc gacatcgcga atctacacaa ggccgtgaag accgcagcgc gacacgagcg
    10081 cggcaacttc tacctcaacc acttcggcgc catctttctc aacggcgtcg gtgggatgat
    10141 cctctggcac tacagcgacg gcaagaaggc actgatcaac gcggtgaccg gctacccggt
    10201 gggcctgctg agcgtctaca ccgcgccgcg cgggagctgg catctctacc gcgacgctac
    10261 ctggtctgtc ggggtcgtgc cgcaacaagg tgcctggctg ctcaccgtca gcggtgagct
    10321 ctagggtcag acataactcc gagctctagg gtcagacata actctatgac tgggtgccgg
    10381 ttggcgagtt atagagttat gtctgacccc tttctggcag gacgtggaag acttcgcgcg
    10441 ctgctcgaag ccgaccgcgt ccacgtgata gcaacgaggt atgcggtggg cgcttgtcgt
    10501 cttgttgcta ctcgcacagc cggttcgcgc gcacgcggcg tgtgatccga cggaagcgga
    10561 gacgctccgc gcccacctcg agcgtcagag cgatcgcgcg cgcaactgga acatcatctg
    10621 gggtgccgtg ttcggcaccg cggcgatcgg aacgttcacc gtggcggtca ccgacgcgat
    10681 tcccgatctg acacccggcc tttacgccag cagcggcaag gcgacgatcg gcgcactcgg
    10741 tcggctgatc ctaccgctgc gcatcccggt gccgccggtc gatgccgaca cgtgcaccga
    10801 gatcgcgaat ctgcacaagg cgatcaagga agcggccaag cgcgagaagg gcaacttcta
    10861 cgtcaaccac gccggcggca tcatcgtgaa cggtgtcgcc gccgcgatcc tctggaagta
    10921 cgacacgggc gcccacgcgc tgctctcggt cgcgaccggc tatccggtcg gcgtgctcag
    10981 caattacacc gcgccgcgcg gcagctggca catgtatcgc gatgcggact gggcgatcac
    11041 cgtcacgccg ccgcagagcg cgtccaactc gccgtggatg ctcacgctcg gcggcgggtt
    11101 ctagccagaa caacggagtg cagcatggag atggcctatc gagaccggtg gcgcgctgag
    11161 agtgccgagc tgcaacggat cttgtctggg tttgatttgc gagtggagcg caagtggggc
    11221 aagccctgct acacgctgaa cggtaagaac gtcgtcctca ttcagggctt caaggagtcc
    11281 tgcgcgttgc tcttcttcaa gggcgcgctg ttgaaagacc cgaagaagct gttggtgcaa
    11341 ctcgggcggg ttcacgcggc ccgggtgatg aagttcgcca gcgccgagga gattgctcgg
    11401 aaggcggcaa cgatcaaggc ctacgtacgc caggctgtgg cggccgagaa ggccggattg
    11461 aaggtcgaga cgaagccccg agagtttccc gtgcccgcgg agctcgagga gaagttccgc
    11521 aagaacccgc ggttcaagcg cgcgttcgag gcgctcacgc cggggcggca acgcggatac
    11581 ttgtttcact tcggcggcgc gaagcagtcc gagacgcgga ccgcgcggat cgagaaggcg
    11641 atgcctgcga tcttcgaagg aagaggcttc ctggagcggc gatagggcta cagcttggcg
    11701 cgatcgtgtc gacgaacgcg gatgcgtcgg cctggaagtc gccggcgtcc gcaagaaagg
    11761 ggggcagacc ggcctgacct cttttcgccg cagagcgccg caaactcgcc gtggatgctc
    11821 acgcgcggcg gcgggttcta gggtgctagc cgcggcttga acgaggcagc gcgtagacgc
    11881 cgccgttgac atggccagtc cagaacagat gcgtatcatc cgccacgatc gacagcgact
    11941 tgtgcggaaa cttcgcgacg agtgcaggcg ttggatcgtc gattcgcatg taccagagcc
    12001 cctgcttgtt gggctcccat gcaccccagt acagcgtgtc gccgcggcgg atcatgcccg
    12061 tcgcgatgcg tggcgttgcg agcgtgcgcg ggaggcggtc cttcgcgctt gcgacaagcc
    12121 cgttctgtgt ccacgcgatc tcgtcgggac ctagcaccac gatgctgccg ggccattcag
    12181 cctgtgcgag cgcgcgggtc gcaccatcgg gctcgcgcac acggatcagt tgatgctgat
    12241 agtagtagag cctaccgtca tggacggtga gtgcgctggt ggggccacga acgagccact
    12301 cgagctcgcc accggtgcgc gggatgcgga acgcgccgat ttcgttctcg tcgcgaccga
    12361 cgccctgggt gccgtagaga aactcctcgt cgcagcagat gtgcgcgtgg gtgtagggcg
    12421 cgagctgcct gaatgtaccg gacttgtcga cgcgacctag cccgcgatcg cagctgacga
    12481 agatgtcgcg atcgtctgcc cacacatcgc agacgttgct caagctgttc gcgtcgaaga
    12541 ccacgtggcg cccggtgcca tcgcgatgca tcacaaacag gcgcggacaa tcgagcgttc
    12601 ccgagacgat caggtgctcc ttgtcgagtg caagcttgct cgcaccgacc acagggtcaa
    12661 gcacgagcgg tttcggtgcg cgaggtggca tcagcggtgt gggctacagc ttgtcggcaa
    12721 tcgcgtcgac gaatgccgac gcatcggcct ggaagtcgcc ggtgatcggc gagccgtcgc
    12781 cgcgcttctt cgtgagcgaa ccttcgcgct cgcacagaat cgtcttctcg gtcttcgtcg
    12841 cgagcacgta gtcgtactcg agggtgaagt agcggttgac cgagccgtcg ttcttcttcg
    12901 tcacgaacgc gcacatatag gcttcgccca tcgcgctcgg ctttggcagc gacatcacgc
    12961 cgaaccgcca gtcgcccttt tccttcgcgc tgacctgtgg cgtgtgctcg acgccggttt
    13021 gctgccacac ctgcgcgaat gccgatgcat cgcgccgttc catcgcgccg atgagcatcg
    13081 cggcgccctt gagcttcagg acgttgggga cgatgaggtg cgcgatcgta tcgagggcac
    13141 tcgccatgcc gtgagcatag ggtcaggcac tacgggttca agttgtgcgg gccacaaaca
    13201 gaacccgtgg tgcctgaccc tattcgcgct attcgcgcta ttcgcgctcg cgcaccgagt
    13261 actcgagctt ccagcgaccg ttgccgtcac gcgcgacgca ccacagctcg agggtgccga
    13321 cctcggtcac gtgcgcttcg agcttcactg gcaccatgtc gccggacttg cggtcgccgt
    13381 cggcggcaac ggccttctcg acgggatcga gctcgacgat gccttcgaca tctgcgtcga
    13441 cgagcgtgcc ggcgccatca tccttgcgcg tcgccgcggc gaagaaccgg aacgtcgacg
    13501 tctcgccgac gacgaggccg agctcgtcat cgggtagctc gacggtcgag ccctcttcga
    13561 gaccttgcgg cgcgacgcat ag
//
LOCUS       DASUFX010000546         7680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1060920, whole genome shotgun sequence.
ACCESSION   DASUFX010000546 DASUFX010000000
VERSION     DASUFX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060920"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..621
                     /locus_tag="VFV99_21795"
     CDS             157..621
                     /locus_tag="VFV99_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062024.1"
                     /translation="MTSPLALFSPMEVNFEIKVPNAAPKTGDYRLLRTVVTSGALGLV
                     ENALPRMIGSQPTRKFGLDAQLTFQYDQNETITLPADAKILALPNPASSSNQVTALSA
                     SCTKKDATTIACARSFQLKSRHIDPNQYKTLRGSLASLTQIARQPVILAGGK"
     gene            623..2623
                     /locus_tag="VFV99_21800"
     CDS             623..2623
                     /locus_tag="VFV99_21800"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX5062025.1"
                     /translation="MKKSLYVAIVLLAACKGSDSSKQSAKAGTKAAAEDPWAKKSASD
                     IKDPKLARLVELATNGPDKDKYPQADAIVALEQDDITYKSDGTVVTKHHSIVKLLDPQ
                     RGKEKYADLHIPYDSKRETLTIETARTVNADGKPQVASKDEIGDIVPAAVADATIYSD
                     VRERVVTFPAVDTGSVLELEYTRTTKATPDSPLGGEEMLAQWNPVLERIVSISAPAGI
                     APKLAVEGQKLEPKKSRAGGNTVWTYKLENQPDRHPEMGSLVEAAVLPRLVFGFQPSW
                     QKVVQPVAERFLKAAVPSPLPASIKAEADRIVAGATTPAEKAQKLFAYVAHDIRSVEL
                     PLGWAGYEPHSPEVVLQNRYADERDKVGLLLALASSQGISGRPVLVRTGKVPVVASVP
                     TIAQFDRVIAKLDVDSKEVWLDPSDEHGQYNVAFAGQDNMVLPLDKGGSELGSRPALD
                     PSTSISATKADFKLAANGDLEATYSYEMSGWYADRAQSELRPLKGELLDRYFQKGAAE
                     LAPTALDKGHEVGDTQSVTGSMSVKQQVTAPGYSTAQANFRVFELPPVTLGVADEVPN
                     AGLSTRKTPLWLGTPRTEKGEITVQVPSGWKVTYVPPKLEGKTEGLSYSDGCEASGQT
                     ITCKAEIKVDKLVVPTDKYGAFREAITKLQAYERRIVLLTRG"
     gene            complement(2620..3966)
                     /locus_tag="VFV99_21805"
     CDS             complement(2620..3966)
                     /locus_tag="VFV99_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062026.1"
                     /translation="MLRRVGLAVLLWSRLAAADRDHDYTLVDDTIEYVTAADERSVAL
                     TTALSSQATGTAGATEGASVATVRAEANVVEIAGTREGYRFALGGEAELRAGIGDVPE
                     LAGSLLARGAIEDTPLFDGGPHFGFGATTVEVQRDYDALPALRDAREWLRAPFDRSRY
                     AVDFTLGRTRDEGSDWTGDTLQLSFASERSRQQNGDIAYTRQVIDLEMMVIRACYGPD
                     SDLFCLRIIEGGGTFPTGASLTTSYFYPVGVDGVPLGDHLRADARLGVLFGNYEIPNT
                     SLGLPDHAPSPTDTCETLGTCVLMRTVGYDLTVRDLDTPHQLHVTRRAYKPMGGEIAI
                     EDRAALATSWQLGKTKLDASAYVAHTRWWTIVDDQFSPQHRGVTYGADVGVSRSLGKR
                     WRVDSTLSAGRSWYGALDGAEPRAGFAAQGTVALAHALRSHWASLQRLRPRQINYY"
     gene            complement(4039..6579)
                     /locus_tag="VFV99_21810"
     CDS             complement(4039..6579)
                     /locus_tag="VFV99_21810"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5062027.1"
                     /translation="MKWALICVALVTACGDDAKTCPGPFGDGDPNGHPDPLGASAGEA
                     RAGRIHAADLPTVPSGLITWQDGDFVLANDKVALVIEDTGDSDLYDPWGGRPVGLARV
                     ENGKLVEPNNFGEVFLLTGRSTVLTESVSVINDGSDGHPAIVRARGKLHPMPFIENLV
                     SVLFNDQYLDIDATIDYQLAPDAEHVDVFMHYSSQRELPTHVPSTLHAVMYTNRTSPS
                     AFQPGLGFDETLNSPYLALVQDDATSWAYSPGDGMLTSSIAASGFLGAFSPGFDIPAC
                     GTAERLHAKIVIGGPGLDGIQAAAARVLGETQRTITGTVTRGGVPAPGVHVHAVEGEK
                     YFTRTTTNAAGEFTLHVPADANVHLDAFKPGDAAGSAEVGSGTTATIDLPAQGAIHVT
                     TTENGVGVPVRVQVLATNPPRMPSKYGEQSIAGSRVYVEFPVTGDITLPIAPGSYEVI
                     VSRGYEYEIVRQNVTVTAAQTTQVDALLDHVVDTTGIQCGDFHVHTWRSNDSGDDSVR
                     KVAQAVADGVELPVRSDHEYVDDFKAEIAKLGVEAFAIGFGSVELTSMEIWGHMGVFP
                     LTPDATKPNGGAPHWQTWPTADALDTPFQTLEPPVVFDSVRARSEAPVVIINHPRGGA
                     NYFDYVGFDPATGLADVEGAWDTKFTLVEVFNDSGWQANKNRNVADWFGLLRAGRKVL
                     AVGSSDTHGISSSPVGYPRTCLSLGTDDPRALTANQVRDTLAAGHTMISGGIYVTARL
                     GNAGPGDSVTGAGSPMMVDVTIQAPTWIDVDAIDVVVDGSVVDTITVMPADADPNKPS
                     TRWRGQIPVQVQASGGFVVIAAYGAKALDPIHPGRIPFGVTNPIFVTP"
     gene            complement(6625..>7680)
                     /locus_tag="VFV99_21815"
     CDS             complement(6625..>7680)
                     /locus_tag="VFV99_21815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062028.1"
                     /translation="GIDPRVDHICRKALAKDPSQRWQSAAELAVEIEEVFQETVGYAT
                     GPGQRSRPFRRPLDEEASIESDLRLRRSDIDAFERSLRRQKYVTMMLAIAIVLAGAAV
                     VAWYVMRAPPPLLEEREPNNDSGHANVIAANTDVTGYVGQRMSTQDGDRDTYIVKWPA
                     GSHRVVSVRVTGLPNLDINLAIADGNGHNAQSDDSRIGEGEVLHRRSIDGPLVVTVSE
                     TVPKGQLPIENVSDAYKLTVTEEKLVGETEPNASDADANPLELTQELRGYLDARDDVD
                     QLRWTGADGDYNVIVRAEGLPLTWKLPDGTLRTPGSAKVTLKRGDLIRLERSDRVGTG
                     TLQGRDVMWSIVVMPGQ"
BASE COUNT         1320 a         2535 c         2564 g         1261 t
ORIGIN      
        1 gagcaccggg tacgcatcga tgccgctctc gcgcagcatc gacgtcagca ggatcgaaac
       61 atcgcggcat acgccccagc gcgaattcat cgtctcctgt gcctcgcgcg gcgtgtagcc
      121 ggtacgcgga ccgacgccga ggtgctgtcg tttaacatga cgagcccgct cgcgctgttc
      181 tcgccgatgg aagtgaactt cgaaatcaag gtgccgaatg cagcgccgaa gaccggcgac
      241 taccgcctgc tccgcacggt cgtgacatcg ggcgcgctcg gcctcgtcga gaacgcgctg
      301 ccgcgcatga tcggcagcca gccgacgcgc aagttcggcc tcgatgcaca gctgacgttc
      361 cagtacgacc agaacgagac gatcacgctg cccgccgacg cgaagatcct cgcgctgccg
      421 aatccggcgt cgtcgtcgaa tcaggtcacg gcgctgtcgg cgagctgcac gaagaaggac
      481 gcgacgacga tcgcgtgcgc gcgctcgttc cagctcaaga gccggcacat cgatccgaac
      541 cagtacaaga cgctgcgcgg ctcgctcgcg tcgctgacgc agatcgcgcg ccagcccgtg
      601 atcctcgcgg gaggcaagtg agatgaagaa atcgctctac gtcgcgatcg tgttgcttgc
      661 cgcgtgcaag ggcagcgaca gctcgaagca atccgcgaag gcgggaacga aggccgcggc
      721 ggaagatccg tgggcgaaga agtcggcgag cgatatcaag gacccgaagc tcgcccggct
      781 cgtcgagctc gcgacgaacg gcccggacaa ggacaagtat ccgcaggccg atgcgatcgt
      841 cgcgctcgag caggatgaca tcacgtacaa gagcgatggc acggtcgtca cgaagcatca
      901 ctcgatcgtg aagctgctcg acccgcagcg cggcaaggag aagtacgccg acttgcacat
      961 cccgtacgac agcaagcgcg agacgctgac gatcgagacc gcgcgcaccg tcaacgccga
     1021 cggtaaaccg caggtcgcgt cgaaggacga gatcggcgat atcgtgccag cggccgtcgc
     1081 cgacgcgacg atctactcgg acgtgcgcga gcgcgtcgtg acgttccccg ccgtcgacac
     1141 gggcagcgtg ctcgagctcg agtacacgcg cacgacgaag gcaacccctg actcgccgct
     1201 cggcggtgaa gagatgctcg cgcaatggaa cccggtgctc gagcgcatcg tctcgatcag
     1261 cgcgccggcc ggcatcgcgc cgaagcttgc cgtggagggt cagaagctcg agccgaagaa
     1321 gtcgagggcg ggcggcaaca cggtgtggac atacaagctg gagaaccagc ccgatcgtca
     1381 cccggagatg ggctcgctgg tcgaggccgc ggtgctgccg cgcctggtgt tcggcttcca
     1441 gccgagctgg cagaaggtcg tgcagccagt cgccgagcgg ttcttgaagg ccgccgtgcc
     1501 gtcgccgctg ccggcatcga tcaaggcaga ggccgaccgg atcgtcgccg gtgcgacaac
     1561 gcccgccgag aaggcgcaga agctgttcgc gtacgtcgcg cacgacattc gctcggtcga
     1621 gctgccgctc ggctgggctg gctacgagcc gcactcgccg gaagtcgtgc tgcagaaccg
     1681 ctacgccgac gagcgtgaca aggtcgggct gctccttgct cttgcctcat cgcagggcat
     1741 cagcggccgg ccagtgctcg tccgcaccgg caaggtgccg gtcgtcgcga gcgtgccgac
     1801 gatcgcgcag ttcgatcgcg tgatcgcgaa gctcgatgtc gacagcaaag aggtgtggct
     1861 cgatccgagc gacgagcacg gtcagtacaa cgtggcgttc gcgggccagg acaacatggt
     1921 gctgccgctc gacaagggtg gctcggagct cggctcgcga ccggcgctcg atccgtcgac
     1981 gtcgatctcg gcgacgaagg cagacttcaa gctcgcggcg aacggcgacc tcgaagcgac
     2041 ctatagctac gagatgtccg gttggtacgc cgaccgcgcg cagagtgagc tgcgtccgct
     2101 caagggcgag ctcctcgatc gctacttcca gaaaggcgcg gccgagctgg caccgaccgc
     2161 tctcgacaag gggcacgagg tcggcgacac gcaatcggtg accggctcga tgtcggtcaa
     2221 gcagcaggtc accgcgcccg ggtactcgac ggcgcaggcg aacttccgcg tgttcgagct
     2281 gccgccggtg acgctcggtg tggccgacga ggtgccgaac gccggactgt cgacgcgcaa
     2341 gacgccgctg tggctcggca cgccgcgcac cgagaagggc gagatcacgg tgcaggtgcc
     2401 cagcggctgg aaggtgacgt acgtgccgcc gaagctcgag ggcaagaccg aaggcctgag
     2461 ctactcggac ggttgtgagg caagcggcca gacgatcacg tgcaaggccg agatcaaggt
     2521 cgacaagctc gtcgtgccga cggacaagta cggcgcgttc cgcgaagcga tcacgaagct
     2581 gcaggcgtac gagcggcgca tcgtgctgct cacgcgcggt tagtagtagt tgatctgcct
     2641 cgggcgcagc cgctggagtg acgcccagtg gctgcgcagc gcgtgggcga gtgcgaccgt
     2701 gccttgcgct gcaaagccag cgcggggctc ggcgccatcg agcgcgccgt accagctgcg
     2761 acctgcggac aatgtgctgt cgacgcgcca gcgtttgccg agcgaacgcg agacgccgac
     2821 gtcggcgcca taggtgactc cgcggtgctg cggcgagaac tgatcgtcga cgatcgtcca
     2881 ccaccgcgta tgcgcgacat acgcggatgc gtcgagcttc gtcttgccga gttgccagct
     2941 cgtcgcgagc gcggcgcgat cttcgatcgc gatctcgccg cccatcggtt tgtatgcgcg
     3001 gcgtgtgaca tgcagttgat gaggagtgtc gaggtcgcgc acggtcaggt cgtagccgac
     3061 ggtcctcatg agcacgcagg tgccgagcgt ctcgcacgtg tcggtcgggc tcggcgcgtg
     3121 atccggaagg ccaaggctcg tgtttggaat ctcgtagttc ccgaacaaca cgccgagcct
     3181 cgcgtccgcg cgcaggtggt cgccgagcgg cacgccgtcc acgccgacag gatagaagta
     3241 tgacgtcgtg agggacgcgc ccgtcgggaa cgtgccgccg ccctcgatga tgcgcaagca
     3301 gaacagatcg gagtcaggcc catagcaggc gcggatgacc atcatctcga ggtcgatcac
     3361 ttggcgcgtg taggcgatgt cgccgttctg ctggcgcgaa cgttccgacg cgaagctcag
     3421 ttgcagcgta tcaccggtcc aatcggagcc ttcgtcgcgc gtgcggccga gagtgaagtc
     3481 aaccgcgtag cgagaacggt cgaacggcgc gcgaagccat tcgcgtgcgt cgcggagcgc
     3541 gggcagggcg tcatagtcgc gctgcacctc gacggtcgtc gccccaaatc caaaatgcgg
     3601 accgccgtcg aacagcggcg tgtcctcgat cgcgccacgc gcgagcaggc tgccggcaag
     3661 ctcggggacg tcgccgatgc cggcgcgcag ctcggcttca ccgccgagtg cgaagcgata
     3721 gccctcccgc gtaccagcga tctcgacgac gttcgcttcc gcgcgcaccg ttgcgacgct
     3781 cgcaccttcc gtggcgccgg ctgtgcccgt cgcctgcgac gacaacgctg tggtcagcgc
     3841 gacgctgcgc tcgtcggccg cggtgacgta ctcgatcgtg tcatcgacga gcgtgtagtc
     3901 atgatctcgg tcggccgctg caagccgcga ccacagcaac accgcaagcc cgacacgtct
     3961 cagcatcgct agccacgcta gcgtgcatcg cttgatgccg acgccacgcg ttcgatgttt
     4021 gccgccgtcc gccgaacgtt acggcgtcac gaagatcgga ttcgtcacgc cgaacggaat
     4081 ccggcccgga tgaatcggat cgagcgcctt ggcgccgtag gccgcaatca cgacgaagcc
     4141 gccgctcgcc tggacctgga ccggaatctg accgcgccag cgcgtcgagg gcttgttcgg
     4201 atccgcatcg gcgggcatca cggtgatcgt gtcgaccacg ctgccgtcga cgacgacatc
     4261 gatcgcatcg acatcgatcc acgtcggagc ctggatcgtc acgtcgacca tcatcggcga
     4321 gccggcgccg gtcacgctgt cacctggccc cgcgttgccg agccgcgcgg tgacgtagat
     4381 gccacccgag atcatcgtgt gaccagcggc gagcgtgtcg cgcacctggt tcgcagtcag
     4441 tgcgcgcgga tcgtccgtgc cgagcgacag gcacgtgcgc ggatagccga ccggcgacga
     4501 cgagatgcca tgcgtgtccg acgagccgac ggccaagacc ttgcggcccg cgcgcaacag
     4561 gccgaaccag tcggcgacgt tgcggttctt gttcgcctgc cagcccgagt cgttgaacac
     4621 ctcgacgagc gtgaacttcg tgtcccacgc gccttcgacg tcggcgaggc cagtcgccgg
     4681 atcgaagccg acgtaatcga agtagttcgc gccgccgcgc gggtgattga tgatgacgac
     4741 cggcgcttcg ctgcgcgcgc gcacgctatc gaacacgacc ggtggttcga gcgtctggaa
     4801 cggcgtgtcg agcgcgtcgg ccgtcggcca cgtctgccag tgcggcgcgc cgccattcgg
     4861 cttcgtcgcg tccggcgtga gcgggaacac gcccatgtgt ccccagatct ccatcgacgt
     4921 cagctcgacc gagccgaagc cgatcgcaaa cgcttcgacg ccgagcttcg cgatctcggc
     4981 tttgaagtcg tcgacgtatt cgtggtcgga acgcaccgga agctcgacgc catcggcgac
     5041 cgcttgcgcg accttgcgca cgctgtcgtc gcccgagtcg ttcgaacgcc aggtgtgaac
     5101 gtggaaatcg ccgcactgga tcccggtcgt gtcgacgacg tgatcgagca gcgcgtcgac
     5161 ctgcgtggtc tgtgcggcgg taaccgtcac gttctgccgc acgatctcgt actcgtagcc
     5221 gcgggacacg atgacttcgt aactgcccgg tgcgatcggc agcgtgatgt cgccggtgac
     5281 cggaaattcg acgtagacgc gcgaaccggc gatcgactgc tcgccgtact tgctgggcat
     5341 gcgcggcggg ttcgtcgcga gcacttgcac gcgtacgggc acaccgacgc cgttctccgt
     5401 cgtggtcacg tggatcgcgc cttgcgcggg caagtcgatc gtcgcggtcg tgccactgcc
     5461 gacctcggcg ctgcctgcag catcgcccgg tttgaacgcg tcgagatgca cgttcgcatc
     5521 ggccggcacg tgcagcgtga actcgccggc ggcgttcgtc gtggtgcgcg tgaagtattt
     5581 ctcgccctcg accgcatgca cgtgcacgcc cggcgctggt acgccaccgc gcgtcaccgt
     5641 gcccgtgatc gtgcgctgtg tctcgccgag cacgcgcgcc gcggccgcct ggatgccgtc
     5701 gagaccgggg ccgccgatga cgatcttcgc gtgcaggcgc tcggcggtac cgcaggcggg
     5761 aatgtcgaag cccgggctga atgcaccgag gaagccgctc gcggcgatcg agctcgtcag
     5821 catgccgtcg ccaggcgagt aggcccagct cgttgcatcg tcctgcacga gcgcgagata
     5881 cggactgttg agtgtctcgt cgaagccgag gccaggctgg aacgccgacg gcgacgtgcg
     5941 gttggtgtac atcactgcgt gcagcgtcga tggcacgtgc gtcggcaact cgcgttgcga
     6001 cgagtagtgc atgaacacgt cgacgtgctc ggcgtcgggc gcgagctggt aatcgatcgt
     6061 cgcatcgatg tcgaggtact gatcgttgaa cagcaccgac acgaggttct cgatgaacgg
     6121 catcggatgc agcttgccgc gcgcgcgcac gatcgccgga tggccgtcgc tgccgtcgtt
     6181 gatcaccgag acgctctcgg tcagaaccgt cgagcggccg gtcaacagaa atacttcgcc
     6241 gaagttgttc ggctcgacga gcttgccgtt ctcgacgcgc gcgaggccga ccgggcgacc
     6301 gccccacgga tcgtagaggt cgctgtcacc ggtatcttcg atcacgagcg cgaccttgtc
     6361 gttcgcgagc acgaagtcgc cgtcctgcca cgtgatcaat cccgacggca ccgtcggcag
     6421 gtcggcagcg tgaatgcgac ctgcgcgggc ttcgcctgcg ctcgcaccga gtggatcggg
     6481 atgaccgttt ggatcaccat cgccgaacgg accaggacac gtcttcgcgt catcaccaca
     6541 tgcagtgacg agcgcgacac aaatgagagc ccatttcacg ggcgaatcgt aacgcaggcc
     6601 gagcagcact cgtcgtcgat tcgtttactg accaggcatc actacgatcg accacattac
     6661 atcgcgtccc tgcaatgtgc cggtgccaac acgatcactg cgttccaatc ggatcagatc
     6721 gccacgcttc agcgtgactt tcgccgagcc cggcgtgcgc agcgtgccgt cggggagctt
     6781 ccacgtgagc ggcagcccct ccgcgcgcac gatcacgttg tagtcgccat cggctccggt
     6841 ccatcgcagc tggtcgacgt cgtcgcgcgc gtcgagatag ccgcgaagct cttgcgtcag
     6901 ctcgagcgga ttcgcgtcgg catcgctcgc gttcggttcg gtctcgccta cgagcttttc
     6961 ttcggtgacg gtgagcttgt acgcgtcgct cacgttctcg atcggcagct ggcccttcgg
     7021 caccgtctcg ctcaccgtga cgacgagcgg gccatcgatc gaacggcgat gcagcacttc
     7081 gccttcgccg atccgactat cgtcggattg cgcgttgtgt ccgttgccgt cggcgatcgc
     7141 gagattgatg tcgaggttcg gcaggccggt cacgcgcacg ctgacgacgc ggtggctgcc
     7201 cgcaggccac ttcacgatat aggtgtcgcg atcgccgtcc tgcgtcgaca ttcgctggcc
     7261 gacgtagccg gtgacgtcgg tgttcgccgc gatcacgttc gcgtgaccgc tgtcgttgtt
     7321 cggctcgcgc tcttcgagta gtggcggcgg cgcgcgcatc acgtaccacg ccacgacggc
     7381 ggcaccggcg agcacgatcg cgatcgcgag catcatcgtg acgtacttct gccgccgcag
     7441 gctgcgctcg aacgcgtcga tgtcggaccg ccgcaggcgc agatcactct cgatgcttgc
     7501 ctcttcatcc agcggccggc ggaacggcct actgcgctga ccgggcccgg tcgcgtaacc
     7561 gaccgtctcc tgaaacacct cttcgatctc gacggcgagc tcggccgcgc tctgccagcg
     7621 ctggctcgga tccttcgcga gcgccttgcg acagatatga tcgacgcgcg gatcgattcc
//
LOCUS       DASUFX010000547         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1199617, whole genome shotgun sequence.
ACCESSION   DASUFX010000547 DASUFX010000000
VERSION     DASUFX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199617"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="VFV99_21820"
     CDS             <1..1926
                     /locus_tag="VFV99_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062029.1"
                     /translation="CAPKVVDQLNMVANFAGGGRVFSFYAPFRCDYCDSEQRALLEVT
                     KDWEAIKSMKLGERPCPSCKENMYFDEDGSTYFSYMVGQGTFELDHDVIGFLATKLDY
                     RVGTVDQKLHIDKIIEGRVTYLRLTGDLNNTFPREKLAEGMEGLVIVDVTSVPRVEPA
                     GAAEWRGFVQQVTPLVEQFYLVGVQPAFLEKLCDKEGLGTKGTVVEMTLPYMCNSCGS
                     GSGQTLNVAEHQDVLKFATAPELRCPNCKAAMTCTATEQAMTILPMLPKVNMPKDLER
                     QIGMFKARKIERKALAAPTLPKAPGARSAVVPVLLTLLVVVIAAGGAVFYMKSNKQDD
                     EGPYGHGPLVAKSADVQPAWIKPNTTPGMTYCGEDGGALTCVGVSMVSPSQEDAEDEA
                     SDAAVESIAFELGKRITDKKWLAAVPPIYTAARDAKLAALNRDPQSTQARRDVRDGRR
                     AVAHAIKGVTPVARYWEAYEARDGRRFVAYIEVKLAAADAAKMIDNYTRQQTALGATT
                     VTYFPELGWKYPKVSRGAIVLALQPGSLQQLGVAENSVVISVDGRDTPDAETFSKFVV
                     EEREVLEERGGSFRLLVQADSGDPREFSQTFPGRVIEVPIKDGKHHGKGTGGTGGVNV
                     WDRFGGNRGSGRDDPTQ"
     gene            2032..3267
                     /locus_tag="VFV99_21825"
     CDS             2032..3267
                     /locus_tag="VFV99_21825"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062030.1"
                     /translation="MRRGWLLLCLGLLSSRVAVAQQPAGSEDVTKEFQAGVDAYRLGK
                     YDEARQHLQKARSMDPKLPGPHRFLAALGQVQSRWSECIESAHKALELNPASSEASDT
                     RKLYESCRISAGRTPARQELGDSAAVAVTTNVVGATVKINGLTYGGTPLAPRPITAGS
                     LDVEIEKPGWKPAKQTVNAIAGVVTDVIVDLEADPNAQSGTDLEVRTAKIKNGYLVVP
                     PGSGTLTIDEKPAPAPVGDRFELLAGTHTLQLEQSGKDLWRRRVRIAAGQKTTVTPEF
                     VDTAQRSAVETRGFYIVGAGAALLAAGFGTAMISRNAADEARDIARIERTRDASRSLS
                     ETAMISPVRTRADLQDARDKSAKFAVISDGLYAVGFVTAGVGAYFLYKGARQRRDVPP
                     PFAIAPMHGGAMIAKELAW"
     gene            3261..3458
                     /locus_tag="VFV99_21830"
     CDS             3261..3458
                     /locus_tag="VFV99_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062031.1"
                     /translation="MVRWLLVFAFASAMAGAACTLFDDDPPDDSCMADTDCFRAQGEV
                     CDMNAHKCVQKPMADAGVDAP"
BASE COUNT          654 a         1154 c         1239 g          581 t
ORIGIN      
        1 tgcgcgccga aggtcgtcga ccagctcaac atggtcgcga acttcgcggg cggcggtcgc
       61 gtgttcagct tctacgcgcc gttccgttgc gactactgcg attcggagca gcgcgcgctg
      121 ctcgaggtga cgaaggactg ggaagcgatc aagtcgatga agctcggcga gcgcccgtgc
      181 ccgtcgtgca aagagaacat gtacttcgac gaagacgggt cgacgtactt ctcgtacatg
      241 gtcggtcagg gcacgttcga gctcgatcac gacgtgatcg gctttctcgc gacgaagctc
      301 gactaccgcg tcggcaccgt cgatcagaag cttcacatcg acaagatcat cgaggggcgc
      361 gtcacgtacc tgcgcctcac cggcgatctc aacaacacgt tcccgcgcga gaagctcgcc
      421 gagggtatgg aaggcctcgt catcgtcgac gtcaccagcg tgccgcgtgt cgagccggcg
      481 ggtgccgccg agtggcgtgg cttcgtgcag caggtgacgc cgctcgtcga gcagttctat
      541 ctggtcggcg tgcagccggc attcctcgag aagctctgcg acaaggaagg gctcggcacg
      601 aagggcaccg tcgtcgaaat gacgctgccg tacatgtgca attcatgtgg ctccggcagc
      661 ggccagacgc tcaacgtcgc cgagcatcaa gacgtgctca agttcgcgac cgcgcccgag
      721 ctgcgctgcc cgaactgcaa agccgcgatg acatgcacgg cgaccgagca ggcgatgacc
      781 atcctgccga tgttgccgaa ggtcaacatg ccgaaggatc tggagcgcca gatcggcatg
      841 ttcaaggcgc gcaagatcga gcgcaaagca ctcgctgcgc caacgctgcc aaaggctccg
      901 ggtgcgcgct cggcagtcgt accggttctg ctcacgctgc tcgtcgtcgt catcgccgcc
      961 ggcggtgccg tgttctacat gaagtcgaac aagcaagacg acgaggggcc atacggtcac
     1021 ggtccgcttg tcgcgaagag cgcggacgtg cagccggcgt ggatcaaacc gaacacgacg
     1081 ccaggcatga cctactgcgg tgaggatggc ggcgcgctga cgtgcgtcgg cgtgtcgatg
     1141 gtgtcgccga gccaggaaga cgccgaagac gaagcgagcg acgccgcggt cgaatcgatc
     1201 gcgttcgagc tcggcaagcg catcacggac aagaagtggc tcgccgcggt cccgccgatc
     1261 tacacagcgg cgcgcgatgc gaagctcgcc gcgctcaacc gcgatccgca gagcacgcag
     1321 gcgcgtcgtg acgtgcgcga tggccgccgg gctgtcgctc acgcgatcaa gggcgtgacg
     1381 ccggtcgctc gctactggga agcctacgag gcgcgcgatg gccgtcgctt cgtcgcgtac
     1441 atcgaggtca agctcgcggc agcggacgcc gcgaagatga tcgacaacta cacgaggcag
     1501 cagaccgcgc tcggcgcgac gacggtgacg tacttccccg agcttggctg gaagtacccc
     1561 aaggtctcgc gcggcgcgat cgtgctcgcg ctccagcccg gttcgttgca gcagctcggc
     1621 gtcgccgaga actcggtcgt catctcggtc gatggccgtg acacgccgga cgccgagacg
     1681 ttctcgaagt tcgtcgtcga ggagcgcgag gtgctggagg agcgcggcgg atcgttccgc
     1741 ttgctcgtcc aggccgattc gggcgacccg cgcgagttct cgcaaacgtt ccccggccgc
     1801 gtgatcgagg tgccgatcaa agacggcaag caccacggca agggcacggg cggcaccggt
     1861 ggcgtgaatg tctgggatcg cttcggcggc aatcgcggca gcggtcgcga cgatccgacg
     1921 cagtaggcca acggctgacg cgcagcgggt gcgccggcgc ggcgtgctgc gctgcacaca
     1981 acggtggcgc gatttcgcac gcgcctgcca gcagtgatac cgttactcgg catgagacgc
     2041 gggtggctct tgctctgtct cggactgctg tcatcgcggg ttgctgtcgc gcagcagcct
     2101 gcggggtccg aagacgtcac gaaggaattc caagctggtg tcgatgcgta ccggctcggc
     2161 aaatacgacg aagcgcgcca acatctccag aaggcgcgca gcatggatcc gaagctgccc
     2221 gggccgcatc ggtttctcgc cgcactcggc caggtgcaga gtcggtggag cgaatgcatc
     2281 gagagcgcac acaaggcgct cgagctgaac cccgcgtcgt cggaagcgag cgatacccgc
     2341 aagctctacg agtcgtgtcg gatctccgcg ggacgcacgc cggcgcgcca ggagctcggt
     2401 gacagcgccg cggttgcggt gacgacgaac gtcgtcggcg cgacggtgaa gatcaacggt
     2461 cttacctacg gtggtacgcc actggcgccg cgtccgatca cggccggttc actcgacgtc
     2521 gagatcgaga agccaggctg gaagccggcg aagcaaacgg tgaacgcgat cgctggtgtc
     2581 gttaccgacg tgatcgtgga tctcgaggcc gatccgaacg cgcagagcgg gaccgatctc
     2641 gaggtgagga ccgcgaagat caagaacggc tatctcgtcg tgccacctgg cagcggcacg
     2701 ctcacgatcg acgagaagcc cgcaccggct ccggtcggtg atcggttcga gctgctcgcc
     2761 ggcacccaca cgcttcagct cgagcagtcc ggcaaggatc tgtggcggcg ccgcgtgcgc
     2821 attgcggccg gtcaaaagac gaccgtcacg cccgagttcg tcgataccgc gcagcgcagc
     2881 gccgtcgaga cgcgcgggtt ctatatcgtc ggtgccggtg cagcattact tgcggccgga
     2941 tttggtacgg cgatgatctc gcgcaatgcc gccgacgaag cgcgggacat cgcgcgcatc
     3001 gagcgcacgc gcgatgcgtc gcgttcgctg tcggagaccg ccatgatctc gccggtgcgt
     3061 acgcgcgctg atttgcagga cgcgcgcgat aagtccgcca agttcgccgt gatctccgat
     3121 ggcctctacg cggtcggctt cgtcacggcg ggggttggcg cttacttcct ctataaagga
     3181 gcgcggcagc gccgcgacgt acctccgccg tttgcgatcg cgcccatgca cggcggcgcc
     3241 atgatcgcga aggagctcgc atggtgagat ggctcctcgt gttcgcattc gcgtcggcga
     3301 tggcaggtgc tgcctgcacg ctgttcgacg acgatccgcc cgacgactcg tgcatggccg
     3361 acaccgactg cttccgcgcg caaggcgagg tttgcgacat gaacgcgcac aagtgcgtgc
     3421 agaagccgat ggctgacgca ggagtggatg caccatgaaa aaggtcctcg cactcctgct
     3481 cgtgagcagc actgcgtacg ccggtggcac cgcacgcccg aacggaatct cggcacgcgg
     3541 cgtcggcatg ggcggcgcct gggtcgcgtt tgcggacgac gtgacagcgg tctacttcaa
     3601 cccggccgcg atggacgcga tgccggcg
//
LOCUS       DASUFX010000548        15007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2033724, whole genome shotgun sequence.
ACCESSION   DASUFX010000548 DASUFX010000000
VERSION     DASUFX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033724"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFV99_21835"
     CDS             complement(<1..717)
                     /locus_tag="VFV99_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX5062032.1"
                     /translation="MPDEPAIAKRKADHLEVAASGRADFARSTLLEQVHLVHQALPEL
                     ALDDIELATELCGKQLRAPLVITGMTGGTAEASVVNKDLARAAQKAGVALGLGSQRAM
                     AEHPELAASYQVRDVAPDVVLFGNVGAVQAKALGAARVVELAKSIGADALCIHLNPGQ
                     ELIQENGDRDFRGLVDAIARVVDTGALPVIVKETGCGLSPQAARALVGAGIHTVDTAG
                     AGGTSWVAVEAVRAADGSDSA"
     gene            complement(730..993)
                     /locus_tag="VFV99_21840"
     CDS             complement(730..993)
                     /locus_tag="VFV99_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062033.1"
                     /translation="MAALDPAYEELFLGIAHALFVNRLHVLRLTEVVRLGIKPDPHDQ
                     NMEVPQEIDRELIQQAFAYVLRHFPQSFAGKIESAKARWIRLA"
     gene            complement(1031..2566)
                     /locus_tag="VFV99_21845"
     CDS             complement(1031..2566)
                     /locus_tag="VFV99_21845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5062034.1"
                     /translation="MSPRINAITLLVIAAAACTANRRRTPDDTIVLLIEAQMNTVDPR
                     YTTSNYETKVSRLIAPGLTTVDTPTAEPELELAAAVDRIDPLTIDVTVRDTTFSDGNP
                     VRAEDVARTFQTVMDDKCESLYQKGLVERFVSVEAVDAKRVRFHLKSPLAMFMSDMDF
                     GIVSFHGTPAGVCKPAKLVGAGPYALHSLGSQGAVLDANPRYFRGSPKIPHVEIRVVR
                     DAAARILMLVGGSADLVQNSFRLDLVDDVIKRPRVQVASAPSVLLTYMLINNTDPVLE
                     DVRVRQAIALALDRDAIVAAKLSGRAVLATGLLPPMHWAYNGNVTHWNHDVARAKQLL
                     DEAGLKPNARGIRLHLVYKTSADAFRIAIARVIAAQLATVGIDVEIRSFEFNTFFADI
                     KKGNYQIATMQSPEITEPDFHRWFFHSSNWPGGKGSESSNRWRYKNPEVDRLVDAGRT
                     ELDRDKRKQIYADIQRIIADELPIIPLWHEDNVVLANVTVQGFQIVPNARFIGLIGTS
                     KSP"
     gene            2560..4179
                     /gene="dacB"
                     /locus_tag="VFV99_21850"
     CDS             2560..4179
                     /gene="dacB"
                     /locus_tag="VFV99_21850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5062035.1"
                     /translation="MTSRVVLVLLLVSIVTAPAHSQQAGSAVPPANGSGAGSAAAAGN
                     PATNDDEEGEGSGSGSAIVAPTDPKARPKWLKEQLQAAIAARPLLTAKAKIGVSFVDI
                     TTGTELFAHDGDKGMSLASNAKLLTSVAALGTIGGGFRWRTTVLVDDKALDETTGIVT
                     GDLYLRAHGDPTLSVNDLRAMATELAARGVRTIQGSLMIEADYFDTTIDPPHFNEQPK
                     ERAYFRAPIASFGVSRSAFLVNVIGEPGGGAKVWLEPDAGEHIKLTKTEVTSVVEGRT
                     RIKVDIKPKKDNLEVEVTGQIRFADGHWWAKKRIDDPLRYAAEVFKRALAEKGIKFQK
                     KPIKLGVIPLNAKPLVTHDSMPLSAVIREMNKSSDNYFAESVLKTLGAETRATPAPAS
                     WADGVAAVQAFLAKIGLPPGTYRCDNGSGLFNATEVSAKQMVTLLRAAHKDYRIGPDL
                     LASLPVGGMDGTLAKRWHTSAARGRVRAKTGTLASVTTLAGYVGVDSAHPVAFAIFVN
                     DIPTGQKGPARAMADDMLDAIVAYLEAEATR"
     gene            4195..5964
                     /locus_tag="VFV99_21855"
     CDS             4195..5964
                     /locus_tag="VFV99_21855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062036.1"
                     /translation="MPKDDEGGTSLATVADRPGAKGIVDPDATADMPEGHIPGADVPT
                     RTVAQRPPRTFPTPPTAADHPEPANFTLTTAADALHNEEIDRTRLFIRLGWAISVGAI
                     GVVPLLPSPMPMRIAMVVAMLFGIVVSFGYHQRFADPAKYSGEALQKLAMMSCINAHV
                     AVLFFGVFTMCPLIVVIGFHFLGRTEAARAGKVVVIVGCICYALISGAIVAGLFDDPG
                     VFATPRPLGHVTQAIGAGFVLAVYGLAFITGRVFRGQSLIAIDELARATRVTSQREAL
                     MAELRADLERALRIGGPGRYTDQTVGAFKLGVVLGRGAMGEVYEAVHTTTSEPAAVKL
                     LRRELLSDATTVARFLREAKASGALASPYVVRVLDASSDDAALPYLAMERLHGATLAE
                     LLRRESKLAGATVIEVVRHAGAGIDAAAAAGIVHRDLKPQNLFKTNDGVWKILDFGVA
                     TLGEEASNLTQGGVVGTPSYMAPEQAQGKRVDGRADGYAIAAVAYRCLTGRHPYSGTD
                     TPTLLYAVVHKMPLRPSSIAELHSDVDACLAVALAKNPDDRFATGAAFAAALEAALDG
                     SLDPALRKRAAALLRKLPWEEPS"
     gene            5961..7586
                     /locus_tag="VFV99_21860"
     CDS             5961..7586
                     /locus_tag="VFV99_21860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062037.1"
                     /translation="MTTIRVQPTTLTTAADALRGEEIDRTRAFVRIGWLIAVFVAGAT
                     LVLPGDERIRTALLGTIGVAVLGSLWVYRQLGDRAHYNVRIMHVLAFAAIVCGQLGIL
                     YVGIFSAAPLIVGLGLYFFCRTESFATALGVYLLAALSHGAEAVLVTAGFIDDPGFAP
                     ARSSLPIISLIAGHLTVQQAYAMSFFLARLSRRSSLRAIEQLQRATRVAAQRDVQLAE
                     LRQDLDRALKVGGPGRFTGHHVGSWTLGSVLGRGAMGEVYEATSTGQGGVRDSGARNL
                     PPEAAVKMLRRELLSDPSHVERFFREMRVASSLDSPHVVKVLQASTLEDPIPFLAMER
                     LRGQTLGDLVRQGPVTGPRLRSLVEQVGGVIDRAREAGIVHRDLKPHNLFLTEEGNWK
                     VLDFGVALLGDSSGTLTQGAVVGTPTYMAPEQARGEPVDHRADVYALGAVIYRCLTGR
                     VPFVARDTPSLLYAVVHSMPLQPSSVAPVSAAEEICLAIAMAKDKRERFATTAELAAA
                     FAQAWHGVVEEDLVRRSRILLRQRPWAVAVDEQ"
     gene            7620..8045
                     /locus_tag="VFV99_21865"
     CDS             7620..8045
                     /locus_tag="VFV99_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5062038.1"
                     /translation="MAELKGSKTHTNLKEAFAGESQANRRYLYFAKVADVEGHPDLAG
                     LFRDTAEGETGHAHGHLDYLKRVGDPATGEPIGDTEKNLKASIAGETYEYTQMYPGFA
                     KTAREEGFTEIAEWFETLARAERSHAGRFQKGLDSMNTK"
     gene            8072..8563
                     /locus_tag="VFV99_21870"
     CDS             8072..8563
                     /locus_tag="VFV99_21870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5062039.1"
                     /translation="MTPLALVDFDEQPFRFTATAKLDADPLAVFAELGDMSLWFPLMR
                     RSVWKTGATSGVGAEREVDVATFGKFRERMLAWDTGARVAFTMTGSTSPLMAAMGEDW
                     RLSREDIYTRIDWIVVGKPTLLGRAATPALRAILRVIFMRGCGNLQKRAGTFKREHAK
                     QPA"
     gene            8560..9147
                     /locus_tag="VFV99_21875"
     CDS             8560..9147
                     /locus_tag="VFV99_21875"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX5062040.1"
                     /translation="MSLVRPIKRSDIKGPAIYAHIRDDYRNRVIELKKPRRVFIGEWV
                     ELVFDNRHTLTLQIEETCRLENLTRDEQIQDEIDVNNPLMPNETELAATMFIALPQDE
                     HIKENLRKLVGLDEHVILHIGPHAIRATFEPGRSTEEKISAVQYTRYPMSSEAKAALL
                     TPGTPIAVEIDHPNYRYRVQCSEELRASLAADYGS"
     gene            9154..9672
                     /locus_tag="VFV99_21880"
     CDS             9154..9672
                     /locus_tag="VFV99_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062041.1"
                     /translation="MKRVIFFAALAACSGAQRQADREAQQFDCRERSASYIATKHIGG
                     DEIGVQIDCTEGPRIKRWKVDKNGKREEDTRGLTPGDFDKTWREIDATGWPNLRDCAN
                     GSLEKSDPVYVFDIKDDTNKASFQCQTRDVPYPYNDITDTLDMMASQGRKQLGDDEPA
                     DLKALDQKDKNR"
     gene            9702..10370
                     /locus_tag="VFV99_21885"
     CDS             9702..10370
                     /locus_tag="VFV99_21885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5062042.1"
                     /translation="MTGASRGIGRAIALRLAEAGAEVALWARDSTALRMVAGEISAAR
                     GKARAIVVDVTDSEAVNRASDLVRATMPPIRTVVNNAGVVLRKSVEAITDDEWRRVMA
                     VNLDGTFFVTRAFIPDLEHNQGRVINISSISGREGTSMLSAYCAAKHGVVGLTRALAE
                     ELRAAKVSVNAICPGSVDTAMLREGMPGARPDMSPDDIAKTALFLAHAAPSALTGACI
                     DVYG"
     gene            10382..11062
                     /locus_tag="VFV99_21890"
     CDS             10382..11062
                     /locus_tag="VFV99_21890"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5062043.1"
                     /translation="MEERMSEALSPPDSQALAALPIFPLPNCVLLPGGLLPLHVFEPR
                     YRELTRDCLAGSQLMAVARLRPGYESTYYGRPPVYDRCGVGRIICSEELPDGRFALLL
                     RGVARVEIARELPPERSYRQVEARMLADVICNRDEAVGHHKKLIQLCDRLAEVIEKGG
                     PQLRDLVRAFDEPGACADAIAAALVMDADARQELLEAIDPMVRLQRTLSHVSHMLCEL
                     APCDGAVN"
     gene            complement(11213..12286)
                     /locus_tag="VFV99_21895"
     CDS             complement(11213..12286)
                     /locus_tag="VFV99_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062044.1"
                     /translation="MRRVVLVLALVGCGDDPAPDPMTNPDNGPPAGNPDGMCAVPAEA
                     MPEDVSTPTSVVGTGSPTSCTSKAVVDAVAKGGVITFDCGPDPIVIEMDETAKIVNDT
                     GPKIVIDGGGKVTLSGRGAHRILYMNTCDQAQKWTTSHCQDQDHPQLSIQNLTFVDGN
                     SSGETMDGGGGGAIFARGGRIKIINSRFFNNACEGTGPDLGGASVRVLSQSMGLPAYV
                     VHSTFGGASDYGNACSNGGGLSSIGVSYTVLNSLFTDNKALGNGANPAKSGTPGGGSG
                     GAIYNDGNTFTLTLCGVKMTDNHANEGGGAIFFVSNDRTGTLVIRDSMLSNNPSDGFE
                     TQGYPGIFVLASGPPQVENSTIE"
     gene            12543..13106
                     /locus_tag="VFV99_21900"
     CDS             12543..13106
                     /locus_tag="VFV99_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062045.1"
                     /translation="MATAEELTREKLTRHPTSPYARDYDPADEGRESMPYLEMEITKD
                     LDIRDVSERKATERVLRLVKTVPAKRLAKGSGPERALSDADVELAKVLAQLPNEAQIE
                     QVMDRLTDPSGPKPHPSQLQWVGVVASPPEVGASMMIGTWLRPEFRITTAVTKLIMRE
                     DGMLIETASKSRYIVHPSGDAYFVRDR"
     gene            complement(13103..14155)
                     /locus_tag="VFV99_21905"
     CDS             complement(13103..14155)
                     /locus_tag="VFV99_21905"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEX5062046.1"
                     /translation="MGNTLADGAKVQVKGSSSSYELARQGSIYSCTCPAWRNQGAPIG
                     LRTCKHLRAYLGDAAETARIGSSAPQVSKATAKKQAAAASGKEPPILLAHKWETDHDP
                     TGWWMSEKLDGVRAYWDGEAFVSRLGNQFYAPDWFIADLPADTLDGELWVGRKMFSET
                     MSIVRSGTSGVEWKKVQYVVFDAPNAKGAFEDRIKHAEKVLTKAKSPHARALEHTPCK
                     GVDHLRSELARVEKLGGEGLMLRRPKSMYEVGRSSSLLKVKTFHDTEGRVIGHAPGTG
                     KHKGRLGALIVQLPDGTKFNVGTGFSDAEREDPPKIGAVITFRYQEMTKDGVPRFPSY
                     VGERIDVKLPPAKPRR"
     gene            complement(14155..14724)
                     /locus_tag="VFV99_21910"
     CDS             complement(14155..14724)
                     /locus_tag="VFV99_21910"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEX5062047.1"
                     /translation="MSEWTAELIFEEGTATKFWRARVEGASVHINYGRVGTDGTSQTK
                     DYSSPAQATTEYGKLVADKRRKGYVASDEARGTPEEDDDDDEIDETADAAADDASAVG
                     ADIDADEVDDEREPDDEDEDDIPVSMPRQKPVRLVLEKNGRHVETYLILDRHGLRTDT
                     TETFANPGDALKAFQELEKSLLASGYKKS"
     gene            14812..>15007
                     /locus_tag="VFV99_21915"
     CDS             14812..>15007
                     /locus_tag="VFV99_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062048.1"
                     /translation="MLRPALAATVACSLISSSAFAWPTFGQEPLEQKPANSRAYERVV
                     QKVANMVGDERAYTMASSYKL"
BASE COUNT         2539 a         4990 c         4892 g         2586 t
ORIGIN      
        1 agccgagtcg ctgccgtccg ccgcgcgaac cgcttcgact gcgacccagc tcgtgccgcc
       61 cgcgcccgcg gtatcgaccg tgtggatgcc agctccgacg agcgcgcgcg ccgcttgcgg
      121 cgacaatccg cagccggttt ctttcacgat caccggcagc gcgccggtat cgaccacccg
      181 cgcgatcgca tcgacgagcc cgcggaagtc gcggtcgccg ttctcctgga tcagctcctg
      241 gcccggattg agatggatgc agagcgcatc tgcgccgatc gacttcgcga gctcgacgac
      301 gcgtgccgcg cccagcgcct tcgcttggac agcaccgacg ttgccgaaca gcacgacatc
      361 cggcgcgacg tcgcgcacct gatagctcgc tgcgagctcg ggatgttccg ccatcgcgcg
      421 ctgcgatccg agcccgagcg cgacgccggc cttctgcgcc gcgcgtgcga gatccttgtt
      481 cacgaccgac gcctctgcgg tgccgccggt catcccggtg atcacgagcg gggcacgcag
      541 ctgcttgccg cacagctcgg tcgccagctc gatgtcgtcg agcgcgagct cgggaagtgc
      601 ctgatgcacg aggtgcactt gctcgagcag cgtcgagcgc gcgaagtcag cgcggcccga
      661 agctgccacc tcgaggtggt cggccttgcg cttcgcgatc gccggctcgt caggcacgag
      721 ccatttttac tacgccaacc ggatccagcg agccttcgcg gactcgatct tcccggcgaa
      781 cgactgcggg aagtggcgga gaacgtacgc gaacgcttgc tggatcagct cgcggtcgat
      841 ctcctgtggc acttccatgt tctggtcgtg cggatccggc ttgatgccca gccgtacgac
      901 ctcggtgagc cggaggacgt ggaggcggtt aacgaacagc gcgtgggcaa tcccaaggaa
      961 caactcttcg tacgcagggt ccaacgcggc cataggagcg cacgtaatta acacgttggc
     1021 cggatgtggg ctagggggac ttggaagtac ctatcagccc gataaacctg gcgtttggga
     1081 cgatctggaa gccctgtacc gtgacgttcg caaggaccac gttgtcctcg tgccacagcg
     1141 ggatgatcgg gagctcgtcg gcgatgatgc gctggatgtc tgcgtagatc tgcttacgct
     1201 tgtcgcggtc gagctcggtg cggccggcgt cgaccaggcg gtcgacctcg gggttcttgt
     1261 agcgccagcg gttcgagctc tcggagcctt tgccacccgg ccagttcgac gaatggaaga
     1321 accagcggtg gaaatcgggc tcggtgatct cggggctctg catcgtcgcg atctggtagt
     1381 tgcccttctt gatgtcggcg aagaaggtgt tgaactcgaa cgagcggatc tcgacgtcga
     1441 tgccgaccgt cgcgagctgc gccgcgatca cgcgtgcaat cgcgatgcgg aacgcatcgg
     1501 cgctcgtctt gtagacgagg tgcaagcgga tgcctcgcgc gttgggcttg aggccagcct
     1561 cgtcgagcag ctgcttcgcg cgcgcgacgt cgtggttcca atgggtgacg ttgccgttgt
     1621 acgcccaatg catcggcggc aacaggccgg tcgctagtac cgctcggccg gagagtttcg
     1681 ccgcgacgat cgcgtcgcga tcgagtgcaa gcgcgatggc ctgacgcacg cgcacgtctt
     1741 cgagcaccgg atcggtgttg ttgatcagca tgtacgtgag cagcacgctc ggtgccgacg
     1801 ccacctgcac gcgcggtcgc ttgatcacgt cgtcgacgag atcgaggcgg aacgagttct
     1861 gcacgagatc cgcggagcca ccgacgagca tcaggatgcg cgcggctgca tcgcgcacga
     1921 cgcgaatctc gacgtgcggg atcttcggac tgccgcggaa gtagcgcggg ttcgcgtcga
     1981 gtaccgcgcc ctgcgagccg agcgagtgca acgcgtacgg ccccgcgccg accagctttg
     2041 cgggcttgca tacgcccgcc ggggtgccgt gaaacgacac gatgccgaag tccatgtcgc
     2101 tcatgaacat cgcgagcggc gacttgaggt ggaagcgtac gcgcttggcg tcgactgcct
     2161 cgacgctgac gaagcgctcg accaggcctt tctgatagag gctctcgcac ttgtcgtcca
     2221 tcacggtctg gaacgtgcgc gcgacgtcct cggcgcgcac cggattgcca tcggagaacg
     2281 tcgtgtcgcg caccgtgacg tcgatcgtga gcggatcgat gcgatcgacc gccgccgcca
     2341 gctcgagctc gggctcggcg gtcggcgtgt cgacggtcgt caggcccggc gcgatcaagc
     2401 gcgacacttt tgtctcgtag ttcgacgtcg tgtagcgcgg gtcgaccgtg ttcatctgcg
     2461 cctcgatgag cagcacgatc gtgtcatcgg gcgtgcgacg ccggttcgcg gtgcatgccg
     2521 cggcagcaat aacgagcagc gtgatagcgt ttatacgtgg tgacatcgcg cgtggtgctc
     2581 gtcctgctgc tcgtgagtat cgtcacggca ccggcgcact cgcaacaagc cggcagcgcg
     2641 gtgccgcccg ccaacggatc tggagccgga tcggcagccg ccgccggaaa tcctgcgacg
     2701 aacgatgacg aggaaggcga aggcagcggg tcgggcagcg ccatcgtcgc accgacggat
     2761 cccaaggcgc gcccgaagtg gctcaaggag cagctccagg ccgccatcgc cgcccggccg
     2821 ctgctcactg caaaagcgaa gatcggggtg tcgttcgtcg acatcacgac cgggaccgag
     2881 ctcttcgcac acgacggcga caaggggatg agcctcgcgt cgaacgcaaa attgctgacc
     2941 tcggtcgcgg cgctcggcac gatcggcggc ggctttcgtt ggcgcacgac cgtgctcgtc
     3001 gatgacaagg cgctcgacga gacgaccggc atcgtgaccg gcgacctcta cctgcgcgcg
     3061 cacggcgacc cgaccctgtc ggtcaacgac ctgcgcgcga tggcgaccga gctcgccgca
     3121 cgcggcgtcc gcacgatcca gggcagcttg atgatcgagg ccgattactt cgacaccacg
     3181 atcgatccgc cgcatttcaa cgagcagccg aaggagcgcg cgtactttcg cgcgccgatc
     3241 gcgagcttcg gcgtctcgcg cagcgcgttc ttggtcaacg tgatcggcga gccaggcggt
     3301 ggtgccaaag tctggctcga gcccgacgcc ggcgagcaca tcaagctgac gaagaccgag
     3361 gtgacgagcg tcgtcgaggg ccgcacccgc atcaaggtcg acatcaagcc gaagaaggac
     3421 aacctcgagg tcgaggtcac gggacagatt cggttcgcgg atggtcattg gtgggcgaag
     3481 aaacgcatcg acgatccgct gcgctacgcc gccgaggtct tcaagcgcgc gctcgccgag
     3541 aagggcatca agttccagaa gaagccgatc aagctcggcg tcatcccgct caacgcgaag
     3601 ccgctcgtca cgcacgacag catgccgctg tcagcggtga tccgcgagat gaacaagtcg
     3661 agcgacaact acttcgccga gagcgtgctg aagacgctcg gcgccgagac ccgcgcgaca
     3721 cctgcacctg cgagctgggc tgatggcgtc gcggctgtcc aagcatttct cgcaaagatt
     3781 ggcctcccac ccggcacgta ccgttgcgac aacggctccg gcctgttcaa cgcgaccgaa
     3841 gtttcggcga agcaaatggt cacgctgctg cgtgccgcgc acaaagacta tcggatcggc
     3901 ccggatctcc tcgcatcgtt accagtcggc ggaatggacg gcacgctcgc gaagcggtgg
     3961 cacacgtcag cggcgcgcgg gcgcgtgcgc gccaagaccg gcacgctcgc ctccgtcacg
     4021 acactcgctg gctacgtcgg cgtcgacagc gcacatccgg tagcgtttgc aattttcgta
     4081 aacgacattc caacaggtca aaaaggaccc gctcgcgcga tggccgatga catgttggac
     4141 gcgatcgtcg cgtacctcga agccgaagcg acgcggtaac attgcccgcc agggatgccg
     4201 aaggacgacg agggaggcac gagcctcgcg accgttgccg atcgtcctgg cgcaaaaggg
     4261 atcgtcgacc ccgatgcgac ggccgacatg cccgaaggcc acatccccgg cgccgatgtt
     4321 ccgacgcgca ccgtcgccca acggccacca cgcacgttcc cgacaccgcc gacggcggcc
     4381 gaccatcctg aaccggccaa cttcacgctg acgaccgcag cggacgcgct ccacaacgaa
     4441 gagatcgatc gtacgcggct gttcatccgg ctcggctggg cgatcagcgt cggcgcgatc
     4501 ggtgtcgtgc cgctcttgcc gtcgccgatg ccgatgcgaa tcgcgatggt cgtcgcgatg
     4561 ctgttcggca tcgtcgtcag ctttggctat caccagcgct tcgccgatcc agcgaagtat
     4621 agcggcgaag cgctgcagaa gctcgcgatg atgagctgca tcaacgcgca tgtcgcggtg
     4681 ctgttcttcg gcgtgttcac gatgtgcccg ctgatcgtcg tgatcggctt tcatttcctc
     4741 gggcgcaccg aagcggcgcg agcgggcaag gtcgtcgtga tcgtcggctg tatctgttac
     4801 gcgctgatct ccggcgcgat cgtggccggc ctgttcgacg accccggcgt gttcgcgacg
     4861 ccacgcccgc tcggtcacgt cacgcaagcg atcggcgctg ggtttgtgct cgcggtgtac
     4921 gggctcgcgt tcatcaccgg ccgcgtgttt cgcggacagt cgttgatcgc gatcgacgag
     4981 ctcgcccgcg cgacgcgcgt gacctcgcag cgcgaggcac tgatggccga gctgcgcgca
     5041 gacctcgagc gcgcactgcg catcggcggt cctggccgtt acaccgacca gaccgtcgga
     5101 gcattcaagc tcggcgtcgt gctcggccgc ggcgcgatgg gcgaggtcta cgaagccgtc
     5161 cacacgacca ccagcgagcc ggccgcggtc aagctgctcc gccgcgagct gctctccgac
     5221 gcgacgaccg tcgcgcgctt cctgcgcgaa gcgaaggcga gtggcgcgct cgcatcgccg
     5281 tatgtcgtgc gcgtgctcga cgcgtcatcg gacgatgccg ccctgcccta cctcgcgatg
     5341 gagcgcctgc acggcgcgac gctcgccgag ctgttgcgcc gcgaatcgaa actggccggc
     5401 gcaaccgtga tcgaggtcgt gcgccatgcc ggcgcgggca tcgacgccgc ggcagctgct
     5461 ggcatcgtgc accgtgatct caagccgcag aacttgttca agacgaatga cggcgtctgg
     5521 aagatcctcg acttcggtgt cgcgacgctc ggcgaagaag cgagcaacct cacgcaaggc
     5581 ggcgtcgtcg gcacaccgag ctacatggcg cccgagcaag cgcagggtaa gcgcgtcgac
     5641 ggccgcgcgg atggttacgc gatcgcagcg gtcgcgtatc gctgcctgac cggccgccat
     5701 ccgtatagcg gcaccgacac gccgacgttg ctgtacgccg tcgtgcacaa gatgccgctc
     5761 aggccgtcga gcatcgccga gctgcactcc gacgtcgacg catgcctcgc ggtcgcgctc
     5821 gcgaagaacc cagacgatcg cttcgcgacc ggtgctgcgt ttgccgccgc gctcgaggct
     5881 gcactcgatg gctcgctcga tcctgcgctc cgcaaacgtg ccgccgcgct cctccgtaag
     5941 ttgccgtggg aggagccgtc gtgaccacga tccgtgtgca gccgacgacg ctgacgaccg
     6001 cggccgacgc gttacgcggc gaagagatcg atcggacacg cgcattcgtg cgcattggct
     6061 ggctgatcgc agtgttcgtc gccggcgcga cgctcgtgct ccccggtgac gaacgcattc
     6121 gcacggcgtt gctcggcacg atcggtgtcg cggtgctcgg cagcctgtgg gtctatcggc
     6181 agctcggcga tcgcgcgcac tacaacgtgc gcatcatgca cgtgctcgcg ttcgccgcga
     6241 tcgtatgtgg ccagctcggc atcctctacg ttggcatctt ctcggcggcg ccgctgattg
     6301 tcggcctcgg cctctacttc ttttgccgca ccgagagctt tgcgaccgcg ctcggcgtct
     6361 atttgctcgc cgcactctcg cacggcgccg aagcggtgct ggtcaccgcc ggtttcatcg
     6421 acgaccctgg gttcgcaccg gcgcgcagct cgctaccgat catctcgctg atcgccggcc
     6481 atctcaccgt gcagcaggcg tacgcgatgt cgttcttcct cgcgcgactc tcgcgacgat
     6541 cgtcgttgcg cgcgatcgaa cagctgcaac gcgcgacccg cgtcgccgcc cagcgcgacg
     6601 tccagctcgc cgagctgcgc caggatctcg atcgtgcact caaggtcgga ggtcccggcc
     6661 ggttcaccgg ccaccacgtc ggctcgtgga cgctcggcag cgtgctcggc cgcggcgcga
     6721 tgggcgaagt ctacgaagcg acatcgaccg gccaaggcgg cgtgcgcgac agcggtgcac
     6781 ggaatctgcc gcccgaggct gcggtgaaga tgctgcgccg cgagctgctc tcggatccat
     6841 ctcacgtcga gcggttcttt cgcgaaatgc gtgtcgcgag ctcgctcgac tcaccgcacg
     6901 tcgtcaaggt gctgcaggcc tccacgctcg aggacccgat cccgttcctc gcgatggagc
     6961 ggctacgtgg ccagacgctc ggcgacctcg tgcgccaagg cccggtcacg ggtccgcgcc
     7021 tgcgctcgct tgtcgagcag gttggcggcg tgatcgatcg cgcgcgcgaa gcgggcatcg
     7081 ttcatcgcga cctcaagccc cacaacttgt ttttgaccga ggagggcaac tggaaggtgc
     7141 tcgacttcgg cgtcgcgctg ctcggcgaca gctccggcac gctcacgcag ggcgcggtcg
     7201 tcggtacgcc gacgtatatg gcgcccgagc aggcgcgcgg cgagcctgtc gatcatcgcg
     7261 ccgatgttta tgcgctcggc gcggtgatct accgctgctt gaccggtcga gtcccgttcg
     7321 tcgcgcgcga cacgccgtca ctgctctacg cggtcgttca ctcgatgccg ctgcagccgt
     7381 cgtcggtagc tcccgtgtcg gccgccgaag aaatctgcct cgccatcgcg atggcgaagg
     7441 acaagcgcga gcgatttgcg acaacggccg agctagccgc cgcattcgcc caggcatggc
     7501 acggtgtcgt cgaggaagac ctcgtacggc ggtcgcgcat cctgcttcgc cagcggcctt
     7561 gggccgttgc tgtcgatgag cagtgatggt acgaccgcct cagaaaaaag gagtcttcca
     7621 tggccgagct caagggaagc aagacgcaca cgaatctcaa ggaagcgttc gcaggtgaat
     7681 cgcaggcgaa ccgccgttac ctctatttcg ccaaggtcgc cgacgtcgag ggtcaccccg
     7741 acctcgcggg cctgttccgc gacaccgccg agggcgagac tggccatgca catggtcacc
     7801 tcgactacct gaagcgcgtt ggtgatccgg cgacgggcga gccgatcggc gacaccgaga
     7861 agaacctcaa ggcgtcgatc gccggcgaga cctacgagta cacgcagatg tacccgggct
     7921 tcgcgaagac cgctcgcgaa gagggcttca cggagatcgc cgaatggttc gagaccctgg
     7981 cgcgcgccga gcgttcacac gccggccgct tccagaaggg tctcgactcc atgaacacga
     8041 agtagagcgt agtatccggg tcatgcgcag catgacccca ttggcgctgg tcgacttcga
     8101 cgagcagccg tttcgcttca cggcaaccgc caagctcgac gcagaccctc tcgcggtctt
     8161 cgccgagctc ggcgatatgt cgctgtggtt tccgctcatg cggcggtcgg tgtggaagac
     8221 cggcgccacg agcggcgtcg gcgccgagcg cgaagtcgat gtcgcgacgt tcggcaagtt
     8281 ccgggagcgc atgctcgcgt gggacacggg cgctcgcgtc gcattcacga tgacgggctc
     8341 gacctcgcct ctcatggcgg ccatgggcga ggactggcgc ctcagccgcg aagacatcta
     8401 cacgcgcatc gattggatcg tcgtcggcaa accgacgctg ctcggccgcg cggcgacgcc
     8461 cgcactgcgt gcgatcctgc gcgtcatctt catgcgcggg tgcggcaacc tccagaagcg
     8521 cgcgggtacg ttcaaacgcg agcatgctaa gcaacccgca tgagcctcgt ccgtcccatc
     8581 aagcgatcgg acatcaaggg tccggcgatc tacgcgcaca ttcgcgacga ctatcgcaat
     8641 cgcgtcatcg agctgaagaa gccgcgccgc gtgttcatcg gcgagtgggt cgagctcgtg
     8701 ttcgacaatc gccacacgct cacgttgcag atcgaagaga cctgccggct cgagaacctc
     8761 acgcgggatg aacagatcca ggacgagatc gacgtcaaca atccgttgat gccgaacgag
     8821 accgagctcg cggccacgat gttcatcgcg ctgccgcagg acgagcacat caaagaaaac
     8881 ctgcgcaagc tcgtcggcct cgacgagcac gtcatcttgc acatcgggcc acacgcgatt
     8941 cgcgcgacgt tcgagcccgg ccgctcgacg gaagagaaaa tctcggccgt gcagtatacg
     9001 cgctacccga tgtcctccga ggcgaaagct gcgctcctga ccccggggac cccgatcgcg
     9061 gtcgagatcg atcacccgaa ctaccgctac cgcgtacaat gctccgaaga gctgcgcgcc
     9121 tcgctcgcag ccgactacgg aagctgacgg gacatgaaac gggtgatttt tttcgccgcg
     9181 ctcgcggcat gcagtggtgc gcaaagacag gccgaccgcg aggcgcagca gttcgattgc
     9241 cgcgaacgct cggcgagcta catcgcgacg aagcacatcg gcggtgacga gattggcgtc
     9301 cagatcgact gcaccgaggg cccgcgcatc aagcgctgga aggtcgacaa gaacggcaag
     9361 cgagaggaag acacgcgcgg cctcacgccc ggtgacttcg acaagacgtg gcgcgagatc
     9421 gacgcgaccg gttggccgaa cctcagggat tgcgcgaatg gaagcctcga gaagagcgac
     9481 ccggtctacg tcttcgatat caaggacgac acgaacaagg cgtcgttcca gtgccagacg
     9541 cgcgacgtgc cctaccccta caacgacatc accgacacgc tcgacatgat ggcttctcag
     9601 ggtcgcaagc agctcggcga tgacgagccg gctgacctca aggcgctcga ccagaaagac
     9661 aagaaccggt gagtagacca ctcgccgcat cgaccgctgt cgtgaccggt gcgagccgcg
     9721 gcatcgggag agcgatcgcg ttacgactgg ccgaggccgg ggccgaggtt gccttgtggg
     9781 cgcgcgactc cacggcacta cggatggtgg ccggtgaaat ttccgccgca agaggcaaag
     9841 ctcgtgcgat cgtcgtggac gtgactgact cggaggcggt caaccgcgca tccgatctcg
     9901 tgcgtgcaac catgccgccg atccgtacgg tcgtgaacaa tgcgggtgta gttctgcgca
     9961 aatcggtcga agcgatcacc gacgatgaat ggcggcgcgt gatggccgtc aacctggatg
    10021 gcacgttctt cgtgacgcgc gcattcatcc ccgacctcga acacaatcag ggtcgcgtca
    10081 tcaacatctc gtcgatatcg ggccgcgagg gcacgtcgat gctctcggcg tattgcgcgg
    10141 caaagcatgg ggttgtcggc ctgactcggg cgcttgccga ggagctacgc gctgctaagg
    10201 tctcggtgaa tgcaatctgc cccggcagcg tggacacagc gatgttgcgc gaaggaatgc
    10261 cgggcgcgag gcctgacatg agtccggacg acatcgcaaa gacggcgttg ttcctggcgc
    10321 acgcagcacc gagtgcgctt accggcgcct gcatcgacgt gtacgggtaa ccttcggagg
    10381 catggaggag cgaatgagcg aagcgctgtc ccctccagat tcacaagccc tcgctgcatt
    10441 accgatcttt ccgttgccga attgcgtgct actgcccggc ggcctgttgc cactgcacgt
    10501 gttcgagccg cgctatcgcg agctcacgcg cgactgcctc gccggttccc agctgatggc
    10561 ggtcgcacgc ctcaggcctg gctacgagtc gacgtactac ggtcgtccgc cggtctacga
    10621 tcgctgcggc gtcggccgca tcatttgctc cgaggagctg cccgatggcc ggttcgcgct
    10681 gctgctccgc ggtgtcgcgc gtgtcgagat tgcgcgcgag ctgccacccg agcgctcgta
    10741 tcggcaggtc gaagcgcgca tgctcgctga tgtgatctgc aatcgcgatg aagcggtcgg
    10801 ccatcacaag aagctgatcc agctctgcga ccgactcgcc gaggtgatcg agaagggcgg
    10861 cccgcagctg cgcgacctcg tgcgcgcgtt tgacgagccc ggcgcctgcg cggatgcgat
    10921 cgccgcagcg ctcgtgatgg atgccgacgc gcgccaggag ctgctcgaag cgatcgatcc
    10981 gatggttcgc ctgcagcgca cgctcagtca cgtcagccac atgctgtgcg agctggcgcc
    11041 ttgcgacggc gccgttaatt aggctcgcgc ttacgatttc gtgcgaccgg tcgcgccttc
    11101 ctgggttgtg cgggtatgcc agcctgtccc gatgacctcg cggtcctgtc ctacctcgag
    11161 ctgcaagccc acctcgcagc ctgcgcgagc tgcgcgcggc tgatcggcga gctcactcga
    11221 tcgtcgagtt ctccacctgc ggaggaccac tcgcgagaac aaagatcccg ggatagccct
    11281 gggtctcgaa gccgtcgcta ggattgttcg agagcatgga gtcgcgaatg acgagcgtgc
    11341 cggtgcggtc gttgctgacg aagaagatcg cgccgccgcc ctcgttcgcg tggttgtccg
    11401 tcatcttcac gccgcacagc gtgagcgtga acgtattgcc gtcgttgtaa atggcaccgc
    11461 cgctgccgcc gcccggcgta cccgacttcg cagggttcgc accgttgccg agcgccttgt
    11521 tgtcggtgaa cagcgagttc agcacggtgt acgagacacc gatgctcgac aggccaccgc
    11581 cgttgctgca cgcgttgccg tagtcgctag cgccgccgaa cgtcgagtgc acgacgtatg
    11641 caggcaagcc catcgactgg ctgagcacgc gcaccgacgc gccgccgaga tcggggccgg
    11701 ttccctcgca cgcgttgttg aagaagcgcg agttgatgat cttgatgcga ccgccccgcg
    11761 cgaagatcgc gccgccacca ccaccgtcca tcgtctcgcc gctcgagttg ccatcgacaa
    11821 acgtcagatt ttggatcgaa agctgggggt gatcctggtc ctggcaatgc gacgtcgtcc
    11881 acttctgcgc ctgatcgcac gtgttcatgt acaggatgcg gtgcgcaccg cgccccgaca
    11941 gcgtgacctt gccgccaccg tcgatgacga tcttcgggcc ggtgtcgttg acgattttcg
    12001 cggtctcgtc catctcgatc acgatcggat ccggaccaca gtcgaacgtg atgacgccgc
    12061 ccttggcgac ggcatcgacg accgccttgc tcgtgcagga cgtggggctc ccggtgccaa
    12121 cgacgctcgt cggtgtggac acatcctccg gcatcgcttc ggcgggaact gcgcacatgc
    12181 catcggggtt gcccgccggc ggcccgttat ccgggttcgt catcggatcc ggcgccggat
    12241 catccccgca acccacgagc gctaacacca gtacgacgcg gcgcatacaa gccatcatac
    12301 gcctcggtta ggcggaaaaa tgaactgtcg ggtacaggca cgtggggcac tgggccttcc
    12361 gtgggacgcc gactctccag gcgcagcgcg tcgttcgagg tacttgtgcg ggcacgcgtg
    12421 ttgctggccc ttacagcgcc atggcgggcg cggttctgac ggcgcatacg gtgcacacgc
    12481 gggtcgtgct gcgcaaacgc cgggctgaca gccgggctgg aaccgagccc tgcgacaagc
    12541 gcatggcgac ggcggaggag ctgacccgcg aaaagctgac ccgccacccg acctcgccgt
    12601 atgcccgcga ctacgacccg gccgatgaag gccgcgagtc gatgccgtac ctcgagatgg
    12661 aaatcacgaa ggatctcgac atccgtgacg tcagcgagcg caaggcgacc gagcgcgtgc
    12721 tgcgcctggt caagaccgtc ccggcaaagc ggctcgccaa aggcagcggc ccagagcgcg
    12781 cgctcagcga cgccgacgtc gagctcgcga aggtgctcgc gcagctgccg aacgaggcgc
    12841 agatcgaaca ggtcatggat cggctcaccg atccgagcgg cccgaagccg cacccgagcc
    12901 agctccagtg ggtcggcgtc gtcgcgtcac cacccgaggt tggcgcgtcg atgatgatcg
    12961 gaacgtggct gcggcccgag ttccgcatca cgaccgcggt gacgaagctg atcatgcgcg
    13021 aggacggaat gctgatcgag accgcctcga agagccgcta catcgtccac ccgagcggcg
    13081 acgcgtactt cgtgcgcgat cgctagcggc gcggtttcgc cggcggcagc ttcacgtcga
    13141 tgcgctcgcc gacgtaactc ggaaagcgcg gcacgccgtc cttcgtcatt tcctggtagc
    13201 ggaacgtgat caccgcgccg atcttcggcg ggtcctcgcg ctcggcgtcc gagaagccgg
    13261 tgccgacgtt gaacttcgtg ccgtccggca actgcacgat caacgcgccg agcctgccct
    13321 tgtgcttgcc ggtgccgggc gcgtgaccga tgacgcggcc ttcggtgtcg tggaacgtct
    13381 tcaccttgag cagcgacgac gagcggccaa cttcgtacat cgacttcggc cgccgcaaca
    13441 tgaggccttc gccgccgagc ttctcgacac gagcgagctc gctgcgcagg tgatcgacgc
    13501 ctttgcaggg cgtgtgctcg agcgcgcgcg catgcggtga cttcgctttc gtcagcacct
    13561 tctcggcgtg cttgatgcgg tcctcgaacg cgcccttcgc gttcggtgcg tcgaacacga
    13621 cgtactgcac cttcttccat tcgacgcccg acgttcccga tcgcacgatg ctcatcgttt
    13681 cggagaacat cttgcgaccg acccagagct cgccgtcgag cgtatcggcc ggcaagtcgg
    13741 caatgaacca atcgggtgcg tagaactgat tgccgaggcg agacacgaac gcttcgccgt
    13801 cccagtacgc gcgcacgccg tcgagcttct cgctcatcca ccacccggtg gggtcgtgat
    13861 cggtctccca tttgtgcgcg agcaagatcg gcggctcctt gcccgacgcc gcggccgcct
    13921 gcttcttcgc ggtggccttg ctcacttgcg gtgccgacga gccgatgcgc gccgtctccg
    13981 ctgcatcgcc gaggtacgca cgcaggtgct tgcacgtgcg caggccgatc ggcgcgcctt
    14041 ggttgcgcca cgcggggcac gtgcacgagt agatcgagcc ctggcgcgcg agctcgtacg
    14101 agctgctcga ccctttgacc tgaaccttcg cgccgtccgc gagcgtgttc cccatcagct
    14161 ctttttatac ccggaagcga ggagcgactt ctccagctcc tggaatgctt tcagcgcgtc
    14221 gccgggattt gcgaacgtct ccgtcgtatc ggtgcggagg ccgtggcggt cgaggatcaa
    14281 gtacgtctcg acgtggcggc cgttcttctc gagcacgaga cgcaccggtt tttggcgcgg
    14341 catgctgacc ggaatatcgt cctcgtcctc gtcgtcgggc tcgcgctcgt cgtcgacctc
    14401 gtccgcgtcg atgtcggcgc cgaccgcgga ggcgtcgtcc gctgcggcgt ccgcggtttc
    14461 atcgatctcg tcgtcgtcgt catcctcttc gggagtgccg cgcgcttcgt ccgaggcgac
    14521 atagcccttg cggcgcttgt cggcgacgag cttgccgtac tcggtcgtcg cttgtgccgg
    14581 cgagctgtag tccttggtct gcgacgtccc gtcggtgccg actcgaccgt agttgatgtg
    14641 aacgctcgcg ccctcgacgc gcgcgcgcca gaacttggtg gcggttcctt cctcgaagat
    14701 cagctcggcc gtccactcgc tcatcgctcg ctactatgca cgggagcggc cggattcgcg
    14761 ggcttaggcg gtcgtttagg ccggcgacgc gggtcgtgcg ttttcacggg gatgttgcgc
    14821 cccgccctcg ccgccacggt tgcttgctcg ctgatctctt cctccgcatt cgcctggccc
    14881 acgttcggcc aagagccact cgagcagaag ccggccaatt cgcgggcgta cgagcgcgtg
    14941 gtccagaagg tcgccaatat ggtcggcgac gagcgcgcct atacgatggc gagctcgtac
    15001 aagctca
//
LOCUS       DASUFX010000549         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_506176,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000549 DASUFX010000000
VERSION     DASUFX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506176"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFV99_21920"
     CDS             complement(<1..433)
                     /locus_tag="VFV99_21920"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5062049.1"
                     /translation="MIRVVVVTDRKLFGSDVPGRLAAMLASAPRGSVLLQIREKDLDG
                     GPLLELARALIEVARPAGAPVWINDRVDVALVAGADGVHLPEHGVSIADAKAAAAAVG
                     RTLAIGASRHDAKAVIDAAKQGAALVQFGPLWATPTKGPPLG"
     gene            complement(430..1200)
                     /locus_tag="VFV99_21925"
     CDS             complement(430..1200)
                     /locus_tag="VFV99_21925"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5062050.1"
                     /translation="MSDQWKLGSRTFTSRIIVGTGKYASLDETRAAIAASGAEMVTVA
                     LRRVDLSSGAPTVLDAIDRTKYVLLPNTAGCYTADDAVRTLHLARELGMDEFVKLEVI
                     GDPKTLFPDNAATVEAAKRLVKEGFKVLPYCSDDLITCKRLEEAGCLAVMPLAAPIGS
                     GLGIRNPHNIRLILENVGVPVIVDAGVGTASDAAIAMELGCTAVLMNTGIAGAKKPVM
                     MAEAMRLAVDGGRKAYLAGRMPKKDYANASSPTAGLIE"
     gene            complement(1206..1418)
                     /gene="thiS"
                     /locus_tag="VFV99_21930"
     CDS             complement(1206..1418)
                     /gene="thiS"
                     /locus_tag="VFV99_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX5062051.1"
                     /translation="MSESIEVVINGQTRTVLQGTTVAALIGELGLGDRRVAVERNREV
                     VPRAEHATTVLAAGDRLELVTFVGGG"
     gene            1692..2468
                     /locus_tag="VFV99_21935"
     CDS             1692..2468
                     /locus_tag="VFV99_21935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5062052.1"
                     /translation="MSLGIARPLTPVMPPRPTTPDPRVQGEDPARIRATIARAPLFAA
                     LPISAIEDLTGRVTVRKVSVGSAVVAQDEPGDAMFLIMSGRVKVVMFGESGREVTLSL
                     LRAGDSFGEMSLFDQGPRSAHCLAIEPTTLLVLSRDDLMKHMQAHPRTAINLVSEMAR
                     RLRKADETIAQLALCDVNERLIHRLVALGREEGASGPDGLVVRRRPTQQELANMIGSC
                     RETISRAFNQLARDGLIIPRGRSLVVTPALIERAEKKKAA"
     gene            2624..3787
                     /gene="alr"
                     /locus_tag="VFV99_21940"
     CDS             2624..3787
                     /gene="alr"
                     /locus_tag="VFV99_21940"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5062053.1"
                     /translation="MIRRTRVEIDLQAIVGNAAAVRAVTGTSLYAVVKADAYGHGAVS
                     VATALAQAKAAAGFCVSLVEEGVALRDAGVTLPILVMGASQVGGEDDMVAAKLTPVIS
                     SDEELEALVDVAKRRNVAVDAHLKVDTGMGRLGVAVERAAELAVVAGRNGVRIVGLMT
                     HFANADTDEAADAASLTRTQLARFAEVDRAVTAAGAPIQVRHAANSSGALVFPQARFD
                     FVRCGIALWGNGRWAHPHERDLASDVARDPKHHVQPAMRLVTAIAQLRRVPVGASVGY
                     GATWRAARESRIAVLPCGYADGLPRRASGHAQAAVRGRRVPLVGLISMDIALADVTDV
                     PEAAVGDAAVLLGRASGGQSITAAEYGGWAGLSEYEVTCGMSKRVPRTYVEAR"
     gene            3784..4749
                     /locus_tag="VFV99_21945"
     CDS             3784..4749
                     /locus_tag="VFV99_21945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5062054.1"
                     /translation="MSTRGDDEETTATGEEPTNVAAPKQPAADADAQKALVKKDAPED
                     DGKKKETIARAIVIGVKDAGLPAYRFVDVVGAHLIMIGRAFAWLPRRPFRGRNYLEAM
                     EYIGFGSLPIILLVGVFTGAVTSLQSVNAFRVFGFESFSGGATGKALSVELGPVLTSL
                     MLAGRVSTGIATELGTMRITEQIDALESMAVNPHQYLILPRIGAGILVTPILALIFFV
                     VAMGGAYVVAVLVEGVQQGPWIANLREIVEVRDVVQGCIKAIFFGFMIATIGCYQGYH
                     ATGGGRGVGIGTARAVVIASVATLVMDYFLTDILFHLMPSSRGEW"
BASE COUNT          760 a         1588 c         1623 g          778 t
ORIGIN      
        1 cacccagcgg cggtcccttc gtcggcgtcg cccacagcgg gccgaactgc acgagcgcgg
       61 caccttgctt cgccgcatcg atgaccgcct tcgcgtcgtg gcgcgacgca ccgatcgcga
      121 gcgtgcgtcc aaccgctgcc gccgcggctt tcgcgtccgc gatcgagacg ccgtgctccg
      181 gcagatgcac gccatcggcg ccggccacca gcgcgacatc gacacgatca ttgatccaca
      241 ctggcgcgcc agccggccgt gcgacctcga tcagggcgcg cgcgagctcg agcagcgggc
      301 cgccgtcgag atccttctcg cggatctgga gcagcacgct gccgcgtggc gcgctggcga
      361 gcatcgcggc gagccgcccc ggtacgtcgc taccgaacag cttgcgatcg gtgacgacga
      421 cgacgcgaat cactcgatca ggccagcagt cggcgacgac gcgttcgcgt agtccttctt
      481 cggcatgcgg cccgcgaggt acgccttgcg accgccatca accgcgaggc gcatcgcctc
      541 ggccatcatc acgggcttct tggcgcccgc gatgccggtg ttcatgagga cggcggtgca
      601 gccgagctcc atcgcgatcg cggcgtcgct tgcagtgccg acaccagcgt cgacgatcac
      661 cggcacgccc acgttctcga ggatgaggcg gatgttgtgc gggttgcgaa tgccgagacc
      721 cgagccgatc ggcgcggcga gcggcatcac ggccaggcag cccgcttctt cgagccgctt
      781 gcaggtgatg agatcgtccg agcagtacgg cagcactttg aagccttcct tcacgaggcg
      841 cttcgcggct tcgaccgtcg cggcgttgtc ggggaacagc gtcttcggat cgccgatcac
      901 ttcgagcttc acgaattcgt ccatgccgag ctcgcgtgcg agatggagcg tgcgtacggc
      961 gtcgtcggcg gtgtagcagc cggcagtgtt cggcaagagc acgtacttgg tgcgatcgat
     1021 cgcgtcgagc acggttggcg cgccactcga tagatcgacc cggcgcagcg cgacggtcac
     1081 catctcggcg ccgctggccg cgatggcggc gcgcgtctcg tcgagcgacg cgtacttacc
     1141 ggtgccgacg atgatgcgcg acgtgaacgt gcgcgaaccg agcttccatt gatcagacat
     1201 tttcttcagc ctccaccgac gaaggtgacg agctcgaggc ggtccccagc tgccagcacc
     1261 gtggttgcgt gctcggcgcg cggcacgacc tcgcgattgc gttcgactgc gacgcgacga
     1321 tcaccgagac cgagctcacc gatcagcgcc gcgacggtcg taccttgtag gacggtgcgg
     1381 gtctgtccgt tgatgacgac ctcgatgctc tccgacatcg gcggggaaac taccatgcgt
     1441 tgccggggcg gctcctccca ggccagtcgt ccccacaaag tcaatgacgc gaaaggccag
     1501 gtgtgatgac cacgacatcc gtgccttggg tgccaacgaa atagtgaagc aagcgtcaca
     1561 ggacttatcc ctgagatggg gggcaaagta ctggaattcc gtcggggcca agagatcccg
     1621 atctcgggca cagcgctcgc aatgactggc gctcgtggcc gcagacctcc tcgggccaca
     1681 ggagcttcct tatgtcactc ggaattgctc ggcccctgac cccagtgatg ccgcctcgcc
     1741 cgacgacgcc tgatccgcgt gttcaaggcg aagacccggc tcgcatccgc gccacgatcg
     1801 cgcgcgctcc gctgttcgcg gcgctgccga tctcggcgat cgaagacctg actggacgcg
     1861 tgacggtgcg gaaggtgtct gtgggatcgg cggtggtcgc gcaagacgag cccggcgacg
     1921 cgatgttcct catcatgtcg ggccgcgtga aagtcgtcat gttcggcgag agcggccgcg
     1981 aagtgacgct gtcgttgctc cgcgccggcg attcgttcgg cgagatgtcg ctgttcgatc
     2041 aaggcccgcg ctcggcgcac tgccttgcga tcgagccgac cacgctgctc gtgctatcgc
     2101 gcgacgattt gatgaagcac atgcaggcgc atccgcgcac tgcgatcaac ctggtgtcgg
     2161 agatggcgcg ccgcctgcgc aaggccgacg agacgatcgc gcagctcgcg ctgtgtgacg
     2221 tcaacgagcg cctcattcat cgcctcgtcg cgctcggtcg cgaagagggc gcctcgggcc
     2281 cggacggctt ggtcgtgcgc cgccggccga cccagcaaga actggcaaac atgatcggca
     2341 gctgccgcga gacgatctct cgcgcgttca accagctcgc ccgcgacggc ctgatcatcc
     2401 cgcgtggccg ctcgctcgtc gtcacgcctg cgctgatcga gcgcgccgag aagaagaagg
     2461 ccgcgtaacc gcgacacgca cgtcgtgagc acgagcgtcg ccctgaaaag gcgacgctgt
     2521 ttgcttttcc gacagggtcg cgcaggtacc agtgacgtag atcgcctcgc cttgacatgc
     2581 tcgtcgacgg ggcgccaaat ccacataagt ggttgagggc acgatgatcc gaaggacgcg
     2641 ggtcgagatc gacctgcaag cgatcgtcgg taacgcggca gcagtgcgcg cggtgaccgg
     2701 cacgtcgctc tacgcggtcg tcaaagccga tgcgtacggc cacggcgcag tctcggtcgc
     2761 gaccgcgctc gcgcaggcga aagccgcagc gggcttctgc gtgagcctcg tagaagaagg
     2821 cgtggcactg cgcgatgccg gcgtcacgct gccgatcctc gtgatgggcg cgagccaggt
     2881 cggcggcgag gacgacatgg tcgccgcgaa gctcacgccg gtgatctcca gcgacgagga
     2941 gctcgaggcg ctcgtcgacg tcgcgaagcg gcgcaatgtc gcggtcgatg cgcacctcaa
     3001 ggtcgatacc ggcatgggcc ggcttggcgt agccgtcgag cgcgccgcgg agctcgcggt
     3061 cgtcgccggg cgcaacggcg tgcgaatcgt cggcctgatg actcacttcg cgaacgcgga
     3121 taccgacgag gctgcggatg ccgcgagcct gactcgcacg cagctcgcgc gatttgccga
     3181 ggtcgatcgc gcggtgaccg cggccggtgc cccgatccag gtccgccacg ctgcgaacag
     3241 ttcgggcgcg ctcgtgttcc cgcaggcgcg cttcgacttc gtccgctgcg gcatcgcgct
     3301 gtggggcaac ggccgctggg cgcatcctca cgagcgcgat ctcgcgagcg acgtcgcccg
     3361 cgatccgaaa caccatgtgc agccggcgat gcggctcgtc accgcgatcg cgcagctgcg
     3421 tcgcgtgccg gttggcgcat cggtcggcta cggcgcgacc tggcgtgcgg cgcgagagag
     3481 ccggatcgca gtgctaccgt gtggctacgc cgatggctta ccgcgccgcg cgagcggtca
     3541 tgcgcaggcg gcggtgcgcg gcaggcgcgt gccgctcgtc gggctgatct cgatggacat
     3601 cgcgctcgcc gatgtgaccg acgtgccgga agccgcggtc ggcgatgccg ccgtcctgct
     3661 aggacgcgcg agcggcgggc aatcgatcac cgcggctgaa tacggcggct gggcagggct
     3721 ctccgagtac gaagtcacgt gcggaatgtc caaacgcgtg ccacgcacgt atgtggaggc
     3781 ccgttgagta cgcgcggtga cgacgaagag acgacggcga ccggcgaaga gccgacgaac
     3841 gtcgcagcgc ccaagcagcc ggccgccgac gccgacgcgc aaaaggccct cgtcaagaag
     3901 gacgcacccg aggacgatgg caagaagaag gagacgatcg cgcgcgcgat cgtcatcggc
     3961 gtcaaggatg ccggccttcc cgcgtatcgg ttcgtcgacg tcgtcggcgc gcacctgatc
     4021 atgatcgggc gcgcgttcgc gtggctgcca cgccggccgt ttcgcggccg caattacctc
     4081 gaggcgatgg agtacatcgg cttcggcagc ttaccgatca tcctgctcgt cggcgtgttc
     4141 accggggcgg tgacgtcgct gcagtcggtc aacgcgttcc gcgtgttcgg cttcgagtca
     4201 ttctcgggtg gcgcgaccgg caaggcactg tcggtcgagc tcgggcccgt gctcacgtcg
     4261 ctcatgctcg cgggccgcgt gtcgaccggc atcgcgaccg agctcggcac gatgcgcatc
     4321 accgagcaga tcgacgcgct cgagtcgatg gccgtcaacc cgcaccagta cttgatcctg
     4381 ccgcgcatcg gcgccggcat cctcgtcacg ccgatcctgg cgctcatctt cttcgtcgtc
     4441 gcgatgggcg gcgcctacgt ggtcgcggtg ctggtcgagg gcgtccagca ggggccgtgg
     4501 atcgcgaacc tgcgcgagat cgtcgaggtg cgcgacgtcg tgcagggctg tatcaaagcc
     4561 atcttcttcg gcttcatgat cgcgacgatc ggttgctacc agggctacca cgcgaccggc
     4621 ggtggtcgcg gcgtcggcat cggcaccgcg cgcgccgtcg tgatcgcgtc agtcgcgacg
     4681 ctggtcatgg attacttcct gaccgacatc ctgttccacc tcatgccgtc atcgcgaggt
     4741 gagtggtga
//
LOCUS       DASUFX010000550         8828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_922318,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000550 DASUFX010000000
VERSION     DASUFX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922318"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFV99_21950"
     CDS             complement(<1..229)
                     /locus_tag="VFV99_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062055.1"
                     /translation="MRFGRFLPLVLVFAASRAGADPETSPITNSNYSIELYDGVALGN
                     TAVVGMGGATVALAIGTSGTLFNPSAPAVKPT"
     gene            263..3091
                     /locus_tag="VFV99_21955"
     CDS             263..3091
                     /locus_tag="VFV99_21955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062056.1"
                     /translation="MPTTGDSDGGTAKTEERSTPSSLPAAADSAHAMSSHATVPFKRR
                     FVMPAVVIAVLELLVVGYPIWFFFDLASLGADTLLRTALPVGIVAAIVWWATTTAWLL
                     PLWSAVSARRRGEKVNKDLAARAYRITLKGPVRVLLLRTGVWTAAAGLTGLFLYIYNN
                     WPIERVAELTALAAVHAYVVSCVRAVWWAQILGEVRGRLFAVGSPLKKFDDSHFRRFM
                     LVAMIVAGGVLAAQAAFAYYFVLGSSQQRELLYLQLETYFPVATVVGLVAWTLFARGL
                     TADLRRYLAISRGESRTDAPPAAFIYRRAQALPYRLALLTVVVWAVIALGGAAVARLR
                     LGFELDDTIVLTTATLLLALAGSIYEQLWHRDVLRPLLAHLTQRYRVPVRSIAPSLSL
                     RSKLMLSFGGVVLLACGMALLWGFVQYKTLQTDSVDRQSDIGVRWLRSELQAELGAAL
                     ESPTETSVREALRSIQAKQAKEPEQTSVSVIYYVGPSGELLSLGGGPMSAPKLPWYTR
                     AMIADAARKDAPGRHITLHGAELTGKSARLSVLWRGVPHEVGSFAVFYPSYRGGGESV
                     SRLKELLVFFLVLFGACAGIVAFTVAQFMAPIRRLEQRADAMARGELADPVAAGGEGD
                     EIGRLTLALEEMRRALRDKLRSTEEVNLDLERAVQMRTADLARKNRELAETLDKLNRA
                     QAQIVRSEKLASIGQLVAGIAHEINNPVNAIVNTVGPLQDAVDSIDKDPEAAKDVVEM
                     VKVVQRGAQRTKAIVSALHNYSRTDDESVVDFDIDRSIDDSLELLRHLLKQNVTVVKK
                     YNNPGRVRGHAGQINQVFMNLLTNAAQALSQREAASITIETKGSVNVGDQDGVEVKIS
                     DNGPGIPPDVLPRIWDPFFTTKDVGEGTGLGLSIVHELVERHGGTIECETKVGDGTTF
                     TVRLPRQIQITEQRKRPAEPKPRDAATR"
     gene            3163..3909
                     /locus_tag="VFV99_21960"
     CDS             3163..3909
                     /locus_tag="VFV99_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062057.1"
                     /translation="MKKLVFGGALLAMLASSVAAQTPSQPQPSPQPQQPQPQVQPQPQ
                     RQPPPPLPPAQPAQAQWYRFTLVDGRVIDVQIIGGDANSYYVVTGGAQYSIARAQVAS
                     SVALQAPAGYAPPPPVAYAPPPPPMAQPAGDDRNLLARNPRAAGWVYFLSAYLITAPI
                     ALAKRDNDSDASLGLIPIVGPMLWAGVNDEDDLFEDGWDWLAGFDSLIQVTGVYFILT
                     GSSSRKNTNAVTLSPLSRRGMHGVVLSGSF"
     gene            complement(3913..4911)
                     /locus_tag="VFV99_21965"
     CDS             complement(3913..4911)
                     /locus_tag="VFV99_21965"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEX5062058.1"
                     /translation="MKFKRALLAAVVLGSGAAGCADQDKDLSPADEISAREELVCTAL
                     TDEPSELDECDSIANDDVVEAYVSKKGDPGTDRCGTRHPLLEERIAVEEEVANRMGFA
                     GGSLNATGGTIPVYWHVINNGTSLSQGNIPDSQIASQLSVLNAAYASTGWQFSLVSTD
                     RTTNSSWYTCTGGTCETQMKNALRKGSADDLNIYSNNMGGGLLGWATFPSDYTSKPKM
                     DGVVILFSSVPGGSAAPYNLGDTATHEIGHWMGLYHTFQGGCARQANRGDMVGDTPAE
                     KSAAFGCPEGRNTCSTPGLDPIHNFMDYTDDSCMDHFTAGQDARMDSLFTTYRFGK"
     gene            5264..6778
                     /locus_tag="VFV99_21970"
     CDS             5264..6778
                     /locus_tag="VFV99_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5062059.1"
                     /translation="MWHQPAQSPFRRFAAAQVEGGSGTPGDEPTALRDVPADAGIVLV
                     VDDETAIVESLTKIFRREGLNVLSATDGNAGLDLLRKHRVGVLLTDLMMPNTSGMDLL
                     RAAKTIAPETEVVLMTAYGTVETAVDAMKEGAYDFVTKPLKRAHVVRIVRNALEKQSL
                     LVENRSLKAQLAEKRRRAIIGTSLAWRRTMDIVMQAAPSEATVLLLGESGTGKELLAR
                     ALHDNSARAKGPFVAVNCAAIPESILEAELFGYEKGAFTGASTARDGRFEAAHGGTLF
                     LDEIGEISRHVQVKLLRVLQEGEIERLGGSGKPRRIDVRIVAATNVNLAEEVKGGRFR
                     EDLYYRLNVIPVSVPPLRDRRDDIPLLAQHFVQVYAEKNGKAISGCSPAAIERLTEYG
                     WPGNVRELENAIERAVVLTRAGNTVIDEDALPREIRDASPGTASATSLTFPIGMPLSE
                     IEMRVIHETLRHTRGDKRLTAKLLGIATRTIYRRLEQTGDEPGPGEDVPDEDKE"
     gene            6779..7693
                     /gene="gspC"
                     /locus_tag="VFV99_21975"
     CDS             6779..7693
                     /gene="gspC"
                     /locus_tag="VFV99_21975"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEX5062060.1"
                     /translation="MQDLIKRYFWVLGALAVFVCAIFAAKATSHVVEAKFLGDSAKAP
                     HVQAALPTAQPVKQTRSKDGGPFATRNMFCSECTPAPVVANADPSQIATTSLPLVLLA
                     TMVSTNPKDSYATVVNTENQKQGAYSVGDQLPGSTGNIKEIHYKYVDFDNGGHVERLV
                     LAGATAPVVAVAPAAEQPSGEPVDDLQASIDNGIKKLDDNNYEIDKALVDKVLANPMA
                     VAKGARVVPAVKNGQPDGFKLYAIRPSSVYAKLGLTNGDTLQSINGFELTSADKALEV
                     YTKLREATSLEVNVTRRGKPMTLKYSIR"
     gene            7697..>8828
                     /locus_tag="VFV99_21980"
     CDS             7697..>8828
                     /locus_tag="VFV99_21980"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062061.1"
                     /translation="MKTKYLALLLLVALLPSGAPVFAQPAPPAGGGANAPTPAPGEDE
                     ALYACKKRTGQVAVTFKPETELKDLITWVMGFTCKNFMLDPRIVSTGKKVTVIAPNKM
                     TAQEAYNVFLAALSTMGLTVVPKGNMMRIVESATARSETVPIMKRGVPANQDQMVRYV
                     MRPSYTQTETLRAALDSIKSPAGSVATAGNVIIITDYASQVRDMLTLAKTLDVPGNNE
                     GIYTIPVKNADAAQLAAKINEILGVSGGGAPVGGGGGGGQRGGGRAGAPAPQGAAQFA
                     SDDQGGAVPSKILVDERSNTLIVVSSEAGYQRVKALDDRLDILLDTEGGSAIRVYPLE
                     NALAEELAATLTNVLSGQGQQQGRPGQPGAPQGQPAPRPAGPI"
BASE COUNT         1557 a         2902 c         2895 g         1474 t
ORIGIN      
        1 tcgttggttt gacggctggc gcggatgggt tgaacagcgt gcccgaggtg ccgatcgcga
       61 gcgcgacggt cgcgccgccc atcccgacga cggcggtgtt gccaagtgcg actccgtcgt
      121 agagctcgat cgaatagttg ctgttcgtga tcggcgaggt ttccggatcc gcgccagcgc
      181 gcgaagcggc aaagaccagg actaacggca ggaatctgcc gaaccgcacc acagctacat
      241 cataccggct attcttctcg caatgccgac taccggcgac agcgacggcg gaactgcgaa
      301 gaccgaggaa cggtcgacgc cgtcgagtct gcccgcagcc gccgacagcg cgcacgcgat
      361 gtcgtcgcac gcgacggtgc cgttcaagcg ccggttcgtg atgcctgcgg tcgtgatcgc
      421 cgtgctcgag ctgctcgtgg tcgggtatcc gatctggttc ttcttcgatc tcgcatcgct
      481 cggcgcggac acgttgctgc ggaccgcgtt gccggtcggc atcgtcgcgg cgatcgtgtg
      541 gtgggcgacg acgaccgcat ggctccttcc gttgtggtcc gcggtcagcg cgcggcgccg
      601 gggcgagaag gtcaacaagg acctcgcagc acgcgcgtat cgcatcacgc tcaagggacc
      661 ggtgcgtgtg ctgctgctcc gcaccggcgt gtggactgcc gcggccggcc ttaccggtct
      721 gtttctctac atctacaata actggccgat cgaacgcgtc gccgagctga ccgcgcttgc
      781 cgccgtgcac gcgtacgtcg tgtcgtgcgt gcgagcggtg tggtgggcac agatcctcgg
      841 cgaggtgcgc ggccggttgt tcgcagtcgg ctcaccgctc aagaagttcg acgacagtca
      901 cttccggcgg ttcatgctcg tcgcgatgat cgtcgccggc ggcgtgctcg ccgcacaagc
      961 cgcgttcgcg tactacttcg tgctcgggag cagccagcag cgcgaactgt tgtacctgca
     1021 gctcgagacg tacttcccgg tcgccacggt cgtcggtctt gtcgcgtgga cgttgttcgc
     1081 gcgcgggctg acggcagacc tccgtcgcta cctcgccatc tcgcgcggcg agagtcgcac
     1141 cgacgcgcca cccgcagcat tcatctatcg ccgcgcgcag gcgttgccgt atcggctggc
     1201 gctgctcacc gtcgtggtgt gggcggtgat cgcgctcggt ggcgcggccg ttgcgcgcct
     1261 gcgccttggc ttcgagctcg acgacacgat cgtgctcacg accgcgacac tgctgctcgc
     1321 gctcgcaggc tcgatctacg agcagctctg gcaccgcgac gtgctgcggc cattactcgc
     1381 gcatctgacg cagcggtatc gcgtgccagt gcgcagcatt gcgccgtcgc tgtcgctgcg
     1441 cagcaagctg atgctgtcgt tcggcggcgt cgtgctgctc gcgtgtggca tggcactgct
     1501 ctggggcttc gtccagtaca agaccctgca gaccgactcg gtcgatcgcc agtccgacat
     1561 cggcgtgcgc tggttgcgct cggagctcca ggccgagctc ggcgcagcac tcgaatcacc
     1621 gaccgaaacg tctgtgcgtg aagcgctgcg ctcgatccaa gcgaaacaag cgaaagagcc
     1681 cgaacaaacg agtgtcagcg tcatctacta cgtcgggccg tcaggcgagc tgctgtcgct
     1741 cggcggcggc ccgatgagtg ctccgaagct gccttggtac acacgcgcga tgatcgcgga
     1801 tgcggcgcgc aaggacgcgc cgggcaggca catcacgctg cacggcgccg agctcaccgg
     1861 caagagtgca cgcctgtcgg tgctctggcg aggtgtaccg cacgaggtcg gctcgttcgc
     1921 ggtgttttat ccgagctatc gcggtggcgg tgaatcggtg agtcggctca aagagctgct
     1981 cgtgttcttc ctcgtgctat ttggcgcgtg cgccggcatc gtcgcgttca ccgtcgcgca
     2041 gttcatggcg ccgattcgcc gcctcgagca acgtgccgac gcaatggcgc gcggcgagct
     2101 tgccgatcca gtcgcggcgg gcggcgaggg cgacgagatc ggtcgtctca cgctcgcgct
     2161 cgaggagatg cgccgcgcgc tgcgcgacaa gttgcgatcg accgaagagg tcaacctcga
     2221 cctcgagcgc gccgtgcaaa tgcgcaccgc tgacctcgcg cgcaagaacc gcgagctcgc
     2281 cgagacgctc gacaagctca accgcgcgca ggcgcagatc gttcgctccg agaagctggc
     2341 gtcgatcggt cagctcgttg ccggcatcgc gcacgagatc aataacccgg tcaacgcgat
     2401 cgtcaacacc gtcggtccgc ttcaggatgc ggtcgacagc atcgacaaag atccggaagc
     2461 ggcgaaggac gtcgtcgaga tggtcaaggt cgttcagcgc ggtgctcagc gcacgaaggc
     2521 gatcgtctcg gcgctccata actactcgcg caccgacgac gaaagcgtcg tcgacttcga
     2581 catcgatcgc tcgatcgacg attcgctcga gctactccgt cacttgctca agcagaacgt
     2641 gacggtcgtg aagaagtaca acaacccggg acgcgtccgc ggccacgctg gtcagatcaa
     2701 ccaggtgttc atgaacttgc tcacgaacgc cgcgcaagcg ctctcgcaac gcgaggctgc
     2761 gtcgatcacg atcgagacga agggcagcgt caacgtcggc gatcaggacg gcgtcgaggt
     2821 caagatcagc gacaacggcc cgggcattcc gcccgatgta ctgccgcgga tctgggatcc
     2881 gttcttcacc acgaaggacg tcggcgaagg caccggcctc ggcctgtcga tcgtgcacga
     2941 gctcgtcgag cgccacggtg gcacgatcga gtgcgagacc aaggtaggcg atggcacgac
     3001 gttcaccgtg cgcctgccgc ggcagatcca gatcaccgaa cagcgcaagc ggccggcaga
     3061 gcccaagccg cgcgacgccg cgacccgctg agttacgcgg cgatcgatca gggcggactt
     3121 tgccgcgttg gcacgagccg ttcgggcgat aatggcggtc tggtgaagaa gctcgtcttt
     3181 gggggcgcgc tgctggcgat gcttgcatcg tcggtcgccg cgcaaacgcc gtcgcaaccg
     3241 cagccctcgc cgcaaccgca acaaccgcag ccgcaggtgc agccgcaacc gcagcggcaa
     3301 ccgccaccgc cgttgccgcc cgcgcaaccg gcgcaggcgc agtggtatcg gttcacgctc
     3361 gtcgatggtc gcgtgatcga cgtccagatc atcggcggcg acgcgaacag ctactacgtg
     3421 gtgaccggcg gcgcgcagta ctcgatcgcg cgcgcgcagg tcgcatcgag cgtcgcgctg
     3481 caagcgcccg ctggctatgc accaccgccg cctgttgcct acgcgccgcc accgccaccg
     3541 atggcgcagc cggccggcga tgacaggaat ctgctcgcgc gcaatccacg cgccgccggg
     3601 tgggtgtact tcctgtcggc gtatttgatc accgcgccga tcgcgctcgc gaagcgcgac
     3661 aacgacagcg atgcgtcgct cgggctcata ccgatcgtcg gcccgatgct gtgggccggt
     3721 gtgaatgatg aagatgatct cttcgaagac ggctgggact ggctggccgg ctttgactca
     3781 ctcatccagg tgacgggcgt ctacttcatc ctcaccggca gcagcagccg aaaaaacacc
     3841 aacgccgtga cgctctcacc gctctcgcgg cgaggcatgc acggcgtcgt actcagtggc
     3901 tcgttctagc gactacttgc cgaagcggta ggtcgtgaac aacgagtcca tgcgtgcgtc
     3961 ctggcccgcg gtgaagtggt ccatgcacga gtcgtccgtg taatccatga agttgtggat
     4021 cggatcgagg cctggcgtgc tgcacgtgtt gcggccctcg ggacaaccga acgcagccga
     4081 cttctccgcc ggcgtgtcgc cgaccatgtc gccgcggttc gcctgccgcg cgcagccacc
     4141 ctggaacgta tggtaaaggc ccatccagtg gccgatctcg tgcgtcgccg tgtcgccgag
     4201 gttgtacgga gcggcgctac cacccggcac cgacgagaac aggatcacga cgccgtccat
     4261 cttcggcttg ctcgtgtagt ccgacgggaa cgtcgcccag ccgagcaggc cgccacccat
     4321 gttgttcgag tagatgttca gatcatccgc gctgcccttg cggagcgcgt tcttcatctg
     4381 cgtctcgcac gtgccaccgg tgcacgtgta ccagctcgag ttcgtcgtgc gatccgtgct
     4441 gacgagcgag aactgccagc cggtcgacgc gtacgccgcg ttgaggacgg agagctgcga
     4501 cgcgatctgc gagtccggga tgttgccctg cgacagcgac gtgccgttgt tgatcacgtg
     4561 ccagtagacg gggatcgtgc cgcccgtcgc gttgagcgag ccgcccgcga agcccatgcg
     4621 gttcgcgact tcttcctcga ccgcgatgcg ctcctcgagc agcggatgcc gcgtgccgca
     4681 gcgatccgta cccggatcgc ccttcttgct gacgtacgcc tcgacgacgt cgtcgttcgc
     4741 aatgctgtcg cactcgtcga gctcgctcgg ctcgtcggtc aacgccgtgc acaccagctc
     4801 ctcgcgcgca gaaatttcat ccgcgggtga aagatccttg tcctggtcgg cacagcccgc
     4861 cgcgcccgaa cccaacacca ctgctgccaa caacgctcgc ttgaacttca tcttgatcct
     4921 ctccttggtg cgggcgtcac tgttcacgag gtgggccaac ggctgcgcga attttcgaga
     4981 gctatctaac gattctctcg acgcgtatcg agactgcgat tcatcgcagt ggctactcga
     5041 cttgccgctg ttgtctggga ctcttgctag cgaccgcact caccaagcga acgccagccg
     5101 gtccggaact tcccgagcgc ccgctttgtc agtttggcag gggcgctcgg acgacctgac
     5161 acgatggcac gaatcgacgc gtacgcaagg aaatcttctc tacaatatca atgacatacg
     5221 tggaatctga gctggcattc cgcttgttaa tgtccctgcc ggtatgtggc accagcccgc
     5281 ccagagcccc tttcgacggt ttgctgctgc ccaggtcgag ggcggtagcg gaactcccgg
     5341 tgacgaacca accgcactgc gcgatgtccc cgcggacgcg ggcatcgtgc tcgtcgtcga
     5401 tgacgagacg gcgatcgtcg agtcgctgac gaagatcttt cgccgcgagg gactcaacgt
     5461 cctgtcggcg acggacggca acgccggcct cgatctgctt cgcaagcatc gggtcggcgt
     5521 gctgctcacc gatctgatga tgccgaacac gtcgggcatg gatctgctcc gcgccgcgaa
     5581 gacgatcgcg ccggaaaccg aggtcgtgct catgacggcg tacggcacgg tcgagaccgc
     5641 agtcgacgcg atgaaagaag gcgcgtacga cttcgtcacc aagccgctca agcgcgcgca
     5701 tgtcgttcgc atcgtgcgta acgcgctcga gaagcagtcg ctgcttgtcg agaaccgcag
     5761 cttgaaagcg cagctcgccg agaagcgccg ccgcgcgatc atcggtacgt cgctcgcgtg
     5821 gcgccgcacg atggacatcg tgatgcaggc cgcaccgagc gaggcgacgg tgctgctgct
     5881 cggcgagagc ggtactggca aggagttgct cgcgcgcgcg ctgcacgaca actcggcacg
     5941 tgcgaagggc ccgttcgtcg cggtgaactg cgccgcgatt cccgaatcga tcctcgaggc
     6001 cgagctgttc ggctacgaga agggcgcgtt cacgggcgcg tcgaccgcac gcgatggtcg
     6061 cttcgaagcg gcgcatggcg gcacgctatt tctcgacgag atcggcgaga tcagccgcca
     6121 cgtgcaggtg aagctgctgc gcgtgctgca ggaaggcgag atcgagcgcc tcggcggcag
     6181 cggtaagccg cgccgcatcg acgtgcgcat cgtcgccgcg acgaacgtca accttgccga
     6241 ggaagtcaaa ggcggccgct tccgcgaaga cctctactac cggctcaacg tgatcccggt
     6301 gtcggtgccg ccgctgcgcg atcgccgcga cgacattccg ctactcgccc agcacttcgt
     6361 gcaggtctac gccgagaaga acggcaaggc gatcagcggc tgctcgccgg cagcgatcga
     6421 gcggctcacc gagtacggct ggccgggcaa cgtgcgcgag ctcgagaatg cgatcgagcg
     6481 cgcggtcgtc ctgacgcgtg ccggcaacac ggtgatcgac gaggatgcgc tgccgcgaga
     6541 gattcgcgac gcgtcgccgg gcaccgcgtc ggcgacgtct ctcacattcc cgatcggaat
     6601 gccgctgtcg gaaatcgaga tgcgcgtgat ccacgaaacg ctccgtcata cacgtggcga
     6661 taaacgactg accgcaaagc tgctaggcat tgcaacccgg acgatttatc gccgtctcga
     6721 acagactggc gacgaacccg ggccaggtga ggacgttccg gacgaggaca aagaatagat
     6781 gcaggacttg atcaagcggt acttctgggt cctcggtgcg ctcgctgtct tcgtgtgcgc
     6841 gatctttgcg gcgaaggcga cgagccatgt cgtcgaggcg aagttcctcg gcgattcggc
     6901 gaaggcgccc cacgtccagg ccgcgttgcc gacggcgcag ccggtgaagc agacccgcag
     6961 caaggacggc ggtccgttcg cgacgcgcaa catgttctgc tccgagtgca cgccggcgcc
     7021 ggtcgtggcg aacgcggatc catcgcagat cgcgacgacg agcctgccgc tcgtgctgct
     7081 cgcgacgatg gtgagcacga accccaagga ttcgtacgcg acggtcgtca acaccgagaa
     7141 ccagaagcag ggtgcgtact cggtcggtga tcagttgccg ggctcgaccg gcaacatcaa
     7201 ggagattcac tacaagtacg tcgacttcga caacggcggt catgtcgagc ggctcgtgct
     7261 cgcgggcgcg actgcgccgg tcgtcgcggt ggcgccggcg gccgagcagc cgagcggcga
     7321 gcccgtcgat gatctgcagg cgtcgatcga taacggcatc aagaagctcg acgacaacaa
     7381 ctacgagatc gacaaggcgc tcgtcgacaa ggtcctcgcg aatccgatgg cggtcgcgaa
     7441 gggcgcgcgc gtcgtgccgg cagtgaagaa cggccagccc gacggcttca agctctacgc
     7501 gattcgtccg agctcggtct acgcgaagct cggcctgacg aacggcgaca cactgcagtc
     7561 gattaacgga ttcgagctga cgagcgcgga caaggcgctc gaggtctaca cgaagttgcg
     7621 cgaggcgacg tcgctcgagg tcaacgtcac gcgtcgcggc aagccgatga cgctcaagta
     7681 ctccattcgg tgaattatga agactaagta cctagccctc ctgctcctcg tcgcgcttct
     7741 cccttccggg gcgccggtct tcgcgcaacc ggccccgccc gcgggcggcg gtgccaatgc
     7801 accaacgccg gcacccggcg aagacgaagc gctctacgcg tgtaagaagc gcaccggcca
     7861 ggtcgccgtc acgttcaagc cggagaccga gctcaaggac ctcatcacgt gggtgatggg
     7921 cttcacgtgc aagaacttca tgctcgatcc gcgcatcgta tcgacgggca agaaggtcac
     7981 ggtcatcgcg ccgaacaaga tgaccgcgca ggaggcgtac aacgtcttcc tcgccgcgct
     8041 gtcgacgatg gggctcaccg tcgtgccgaa gggcaacatg atgcgcatcg tcgagtcggc
     8101 gaccgcacgc agcgagaccg tgccgatcat gaagcgcggt gtgccggcga accaggacca
     8161 gatggttcgc tacgtgatgc ggccgtcgta cacgcagacg gaaacgttgc gcgccgcact
     8221 cgactcgatc aagtcgccgg ccggcagcgt ggcgaccgca ggcaacgtca tcatcatcac
     8281 cgactacgcg agccaggtcc gcgacatgct gacgctcgcg aagacgctcg acgtgcccgg
     8341 caacaacgag ggcatctaca cgatcccggt gaagaacgcc gatgccgcgc agctcgcggc
     8401 gaagatcaac gagatcctcg gcgtcagcgg cggcggggca cccgtcggcg gtggtggcgg
     8461 cggtggtcag cgcggcggcg gcagggcagg tgcaccagcg ccgcagggcg ccgcgcagtt
     8521 cgcgagtgat gaccaaggcg gcgcggtgcc gtcgaagatc cttgtcgacg aacgcagcaa
     8581 cacgctgatc gtcgtgtcga gcgaagccgg ctatcagcgc gtgaaggcgc tcgacgatcg
     8641 cctcgacatc cttctcgata ccgaaggcgg ctccgcgatt cgcgtgtatc cgctcgagaa
     8701 cgcgctcgcc gaagagctcg ccgccacgct gacgaacgtg ctgtcgggcc agggtcagca
     8761 gcagggtcgc ccgggccaac ccggcgcgcc gcagggccag cccgccccgc ggccggccgg
     8821 tccgatcg
//
LOCUS       DASUFX010000551         5906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1199758, whole genome shotgun sequence.
ACCESSION   DASUFX010000551 DASUFX010000000
VERSION     DASUFX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199758"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1539
                     /locus_tag="VFV99_21985"
     CDS             13..1539
                     /locus_tag="VFV99_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062062.1"
                     /translation="MSNAPVAKAADHAPAAEHAPPVEEKHDKPKAKPHTDKRLLDHEL
                     NAVLKQFDHGKLDMQAAVARFVAFDQKHSGGEMSADGQVMLAMLVGMLMAHESEKLRK
                     KYESEHHGPTTAPAEQGKPPVINDHEAPAKGPAPVFNPPANPKLEDQNLDALAAQLHD
                     PKVTKVMADLASAVTVWRTAPRDSFHEIKGEPRENLVHGIAVVREGLKGLGGADEKTA
                     AFKTAVFHKLEQIAPYHHQHNMRTIETGDYTTCNVTSLAMSLEAIGKTAESYKPGKRE
                     IIKKVAERCNSSVSKAASTLHGHGASWEAVAGLRFPDFMQLAAISHRLKTPEFSESDF
                     KAAAMFAASNKTHADFLADIASDFSATGKEHYLSFGSEKADKALEKFSDDTQADAQVL
                     IDARNAAEAHPESKVRQDAYAEAQQGRGKKVAGNAKIDRDLPLEHYKATVIGAIKPLM
                     DAGHGVISGCYHHFTRCYEINDKYIMVQDPGQYHRTERKLMWDEARALRYFWNYIVIS
                     "
     gene            1536..2120
                     /locus_tag="VFV99_21990"
     CDS             1536..2120
                     /locus_tag="VFV99_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062063.1"
                     /translation="MTLLRTAVLLVALLACNKKSDTGSGSSGSAGSAAPVGSDGSAGS
                     AGSAGSAGSAGSAAAGTASPELLKALDEMDRVMAPIFKLEPPKRFDAYCASLAELAAK
                     AAAIQAAPAPAGVADWATNAESFKSDVSGPEMCCKEAPKGLKPAEQKGQDLTNDECVT
                     PVHEEFKKLVASVPGAPAADAHANDPLPAAANKK"
     gene            2117..2953
                     /locus_tag="VFV99_21995"
     CDS             2117..2953
                     /locus_tag="VFV99_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062064.1"
                     /translation="MTLRALVAVALALAPSIARADAKADATKAFTDFITSLATEKPAL
                     SDLEAFLPLYLDRAPVPVATDVKSTLEAPKLKVLRADVSKSGTSVWIVAEVKTKSVKV
                     FDDATKNVTLRASGFLVLDKGAWHVRAADLSRAVEDKPADPNCGNASHQWGLEKAPPK
                     ALAATVRMTLTAVDQGKSHAALLSDSKNTLVLGSAPGEQFAGAAAKGVKKWQIRSSGN
                     SDDDANIRALAGIGPDADLMWIAAPISGPAKLCTEYRGFFVLAKEASGWKIVHEHYSS
                     PL"
     gene            2977..3573
                     /locus_tag="VFV99_22000"
     CDS             2977..3573
                     /locus_tag="VFV99_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062065.1"
                     /translation="MKLVIGIVVGVLAVGCSKKSEAPAGGGSSGSNTPAVEPRDALVA
                     TPDGNAAVVDAAIGSAVAPTTDNAIAVDAHAAETIDAASPVRVAGDSKATELRAEVDA
                     VDAAFEKAAKAAADAKTVKAACAGLADLGSAISELADFGPANAEHEWQEARQDAAEKL
                     GQVLVLCNERGRKPAEVVEGLDGIRGELATLRGALRSN"
     gene            3597..4433
                     /locus_tag="VFV99_22005"
     CDS             3597..4433
                     /locus_tag="VFV99_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062066.1"
                     /translation="MKRLAALGVVVACTYHNPKPQYAQPGPPQQPLGQQQPPQPAVAS
                     QPAPPAPVQPAPPPEPAKPPEEPGPIDPNAEMPKDSITTNTKLVLGTEATIKLDARSD
                     IYSATAKKADPGRGGVLPSEVTLAKEGGIISFPKVIGRAACVADAGATADGGDCAGGS
                     TDLTSAGKLSGIKHGQRTMFLVGVFVGAKLPAKAPAVLDFSDDKLGTKFPKLEPVLGQ
                     VFWIGDGKPGEPAGDTHDFVIPKGATRLYLGYADGYGFQGTPGAYGDNKGGLAVTILQ
                     RK"
     gene            4701..5366
                     /locus_tag="VFV99_22010"
     CDS             4701..5366
                     /locus_tag="VFV99_22010"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX5062067.1"
                     /translation="MPTETETKQKWSKQATERVLAALNRADEIGGEVRDFIQDKMTTD
                     PRYIKARKTVAKWLGREYVSRDEAAQRAAAAEAQRKAAAASAPVAKKKDANAGFGDAS
                     LKAQIFGKKSCPWTGRSITLLERHKLDYDYIDLEEPEHENKIVPLSNETKQHTVPFIY
                     LRGQFIGGFNALAEIERLGQLEFALMTAEERAALPEHKRNVEIVARPNTDEVAPAEET
                     PGS"
     gene            5593..>5906
                     /locus_tag="VFV99_22015"
     CDS             5593..>5906
                     /locus_tag="VFV99_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062068.1"
                     /translation="MAIESTAINDLIAGFQQRPLQRDSNDWLFGERDDATEIDSGSEE
                     KATTPFDRSTPPVVPLPQAFVHQPAPALEALYRSPSPTAYVRPISASGVAKKLALPIG
                     VLS"
BASE COUNT         1183 a         1859 c         1972 g          892 t
ORIGIN      
        1 aaccatgcgc ccgtgagcaa cgctcccgtc gccaaagccg cggatcacgc acccgccgcc
       61 gagcacgcgc cgcctgtcga ggagaagcac gacaagccga aagcgaagcc tcacaccgac
      121 aagcgcctgc ttgaccacga gctcaacgcc gtgctcaagc agttcgatca cggcaagctc
      181 gacatgcagg ccgctgtcgc gcgcttcgtc gcgttcgacc agaagcactc gggcggcgag
      241 atgtccgccg atggtcaagt gatgctcgcg atgctggtcg gcatgctaat ggcgcacgag
      301 agcgagaaac tgcgcaagaa gtacgagtcc gagcaccatg gtccgacgac agcgccggca
      361 gagcagggca agccgcccgt gatcaacgat cacgaagccc ctgcgaaggg acccgcgccc
      421 gtcttcaacc cgccggcaaa tccaaagctg gaggatcaga acctcgacgc gctcgctgcg
      481 cagcttcacg atccgaaggt cacgaaggtc atggccgacc tcgcgtccgc ggtgaccgtc
      541 tggcgaacgg cgccgcgcga ctcgttccac gagatcaagg gcgagccgcg tgagaacctc
      601 gtgcacggca tcgctgtcgt gcgtgaaggg ctaaaggggc tcggcggtgc cgacgagaaa
      661 accgcggcat tcaagaccgc ggtgtttcac aagctcgagc agatcgcgcc gtatcaccat
      721 cagcacaata tgcggacgat cgagacgggc gactacacga cgtgcaatgt cacctcgctc
      781 gcgatgtcgc tcgaggcgat cggcaagacc gcggaaagct acaaacccgg caagcgcgag
      841 atcatcaaga aggtcgccga acgctgcaac agcagtgtca gcaaggcggc aagcacgctg
      901 cacggtcacg gcgcttcgtg ggaagcggtc gccggccttc gctttcccga tttcatgcag
      961 ctcgcggcaa tttcccacag gctgaagacc cccgagttca gcgagagtga ctttaaggct
     1021 gctgcgatgt tcgcggcctc gaacaagacc cacgctgact tcctcgccga catcgcgagc
     1081 gatttctcgg cgaccggcaa ggagcactac ctgtcctttg gcagcgagaa ggccgacaaa
     1141 gcgctcgaga aattctcgga cgacacgcaa gcagacgcgc aggtgctgat cgacgcgcgg
     1201 aacgccgctg aagcgcatcc cgagagcaaa gttcgtcagg acgcctatgc agaggcgcag
     1261 cagggacgcg gcaagaaggt cgccggcaac gcgaagatcg atcgcgattt accgctcgag
     1321 cactacaagg cgacggtcat cggggcgatc aagccgctga tggatgcagg ccatggcgtg
     1381 atctcgggct gctaccacca cttcacgcgc tgctacgaga tcaacgacaa atacatcatg
     1441 gtccaggacc cgggccagta tcaccgcacc gagcgcaagc tgatgtggga cgaagcgcgc
     1501 gcactccgct acttctggaa ctatattgtc atctcatgac gctcctcaga actgcggtcc
     1561 tcctcgtcgc gctgttggcc tgtaacaaga agagcgatac aggctcgggc tcttcgggct
     1621 ctgcgggctc ggcggctccc gtcggctcgg atggctccgc aggatccgcc ggatccgcgg
     1681 gctcggctgg atcggcagga tcggcggcag ccggcaccgc gtcgcccgag ctactcaaag
     1741 cgctcgacga gatggatcgc gtgatggctc caatcttcaa gctcgagccg ccgaagcgct
     1801 tcgacgcgta ctgtgcctcg cttgccgagc tggctgcgaa ggcggcggcg atccaggcgg
     1861 caccggcgcc agctggcgtc gctgactggg cgacgaacgc cgagagcttc aagagtgacg
     1921 tctcgggacc cgagatgtgc tgcaaggagg cgccgaaagg tctcaagcct gccgagcaga
     1981 agggacagga tctgacgaac gacgaatgtg tcacgccggt ccacgaagag ttcaagaagc
     2041 tcgtcgcatc ggtgccgggc gcaccggccg ctgacgcgca cgcgaacgat ccactccccg
     2101 cagcggcaaa caagaagtga cgctccgcgc gctcgtcgcg gttgccctcg cgctcgcacc
     2161 gagcatcgcg cgcgccgacg cgaaggccga cgccacgaag gcgttcaccg acttcataac
     2221 gagccttgca accgagaagc cggcgctctc tgatctggag gcgtttctgc ctctgtatct
     2281 ggaccgtgct cccgtgcccg tcgcgactga cgtcaagagc acgctggagg caccgaagct
     2341 caaggtgctg cgcgccgacg tctccaagag cggcacgtcg gtgtggatcg tggccgaggt
     2401 gaagaccaag agcgtgaagg tgtttgacga cgcaaccaag aacgtgacgt tgcgtgcgag
     2461 tggatttctt gtcctcgaca aaggtgcctg gcacgtgcgc gcggcggatc tgtcgcgcgc
     2521 tgtcgaggac aagcccgcgg atccgaattg cggcaacgcg agccaccagt ggggcctcga
     2581 aaaggcacct ccgaaagcgc tcgccgcgac ggttcgcatg acgctcaccg cggtcgacca
     2641 gggcaagagc cacgctgcgc tactgtccga cagcaagaac acgctcgtgc tcggcagcgc
     2701 gcccggtgag caattcgcag gtgcggcggc gaagggcgtg aagaagtggc agatccgctc
     2761 gtcgggaaac agcgatgacg atgcgaatat ccgcgcgctc gccggcatcg ggcccgatgc
     2821 cgacctgatg tggatcgcag cgccgatttc tggccccgcg aagctgtgca ccgagtaccg
     2881 cggattcttc gtgctggcca aagaggccag cggctggaag atcgtgcacg agcactattc
     2941 gtcaccgctg tagcgcgatc gcgtaagctc ggtgccgtga agctcgtcat cggcatcgtc
     3001 gtcggcgtgc tcgctgttgg atgcagcaag aagtccgaag cgcccgcggg tggtggctcg
     3061 agcggatcga atacgccggc ggtggagccc agggacgcgc tcgtcgcgac acctgacggc
     3121 aatgcggcgg ttgtcgatgc ggcgatcggt tccgcggtgg ctccgacgac cgacaacgcg
     3181 atcgcggtcg atgctcacgc agccgaaacg atcgacgccg catccccggt acgcgtcgca
     3241 ggtgactcca aggcaaccga gcttcgtgcc gaggtcgatg ccgtcgacgc cgcattcgag
     3301 aaggccgcga aggccgcagc agatgcgaag acggtgaagg ccgcgtgcgc gggcctcgcc
     3361 gatctcggca gtgcgatcag cgagctcgcc gacttcggtc ctgcgaacgc cgagcacgag
     3421 tggcaagaag cgcggcagga cgcggccgag aagctcggcc aggtactcgt gctctgcaat
     3481 gaaagaggca ggaagccggc ggaagtcgtc gagggcctcg acggcattcg cggcgagctc
     3541 gcgacgctgc gcggggcatt gcggtcgaat tgaacaggtg cgatgaaggt gttccgatga
     3601 agagacttgc agcgttgggg gtcgtcgtcg cgtgcacgta tcacaacccg aagccgcagt
     3661 acgcgcagcc gggaccgccg cagcaaccgc tggggcagca gcaaccaccg caacccgccg
     3721 tcgcgtcgca accggcacca cctgcacccg tgcagcctgc accgccaccg gagccggcga
     3781 agccgcccga ggagccggga ccgatcgatc cgaacgcgga gatgccaaag gattcgatca
     3841 cgacgaacac gaagctcgtg ctcggaaccg aggcgacgat caagctcgac gcgcgcagcg
     3901 acatttactc ggcgaccgcg aagaaggcgg atccgggacg tggcggcgtg ttgccatcag
     3961 aggtcacgct cgcgaaggaa ggcggcatca tttcgtttcc gaaagtgatc ggtcgcgcgg
     4021 cctgtgtcgc ggacgccggt gcgaccgccg atggcggcga ttgcgcgggc ggcagcaccg
     4081 atctgacgag cgcgggcaag ctgagcggca tcaagcacgg ccagcgcacg atgttcttgg
     4141 tcggcgtgtt tgtcggcgcg aagctaccgg cgaaggcgcc ggccgtcttg gacttcagcg
     4201 acgacaagct cggcaccaag ttccccaagc tcgagccggt gctagggcag gtcttctgga
     4261 ttggggacgg caaacccggg gagccagctg gggacaccca cgacttcgtg atccccaagg
     4321 gcgccacgcg gctgtacctc gggtatgcgg acgggtatgg gttccaaggg acaccgggcg
     4381 cctacgggga caacaagggc ggcttggcgg tgaccatcct gcagcgtaag tagccggatg
     4441 tgcggcaacc tcgcgggggc cgaaacaccg tcacctttgt cctggtcgtg aacggcggtt
     4501 ggtgatctac ccggcaaacg aagcgtaggt gagcgctaga tcggctggaa cggcccatgc
     4561 tatggagtcc gctcactatg ggtcgccttt ctattgtcgc gcttagctcc gtggttgttg
     4621 cgatgtcggc tggtgtagtc gggtggatgg cagctcgtgc cttgtccctg ctggtctgag
     4681 catctataac cgccggcgtc atgccgacgg agaccgagac gaagcagaag tggtcgaagc
     4741 aagcgacgga gcgggtgctt gcggcgttga accgagccga tgaaatcggc ggcgaagttc
     4801 gcgacttcat ccaggacaag atgacgaccg acccgcgcta catcaaggcg cgcaagacgg
     4861 tcgcgaagtg gctcggccgc gagtacgtct cgcgtgatga agcagcgcag cgtgccgcgg
     4921 ccgctgaagc gcagcgcaag gcggcagctg ccagcgcacc ggtggcgaag aagaaagacg
     4981 cgaacgccgg cttcggtgat gcgtcgctca aggcgcagat cttcggcaag aagagctgcc
     5041 cgtggacagg ccgctcgatc acgctactcg agcgtcacaa gctcgactac gactacatcg
     5101 atctcgagga gccagagcac gagaacaaga tcgtgccgct ctcgaacgag acgaagcagc
     5161 acacggtgcc gttcatctac ctgcgcggtc agttcatcgg cggcttcaac gcgctcgccg
     5221 agatcgagcg gctcggtcag ctcgagttcg cgctgatgac ggccgaggag cgcgcggcgc
     5281 tccccgagca caagcgcaat gtcgagatcg tcgcgcgccc gaataccgac gaagtcgcgc
     5341 ccgccgaaga gacgccgggc tcctgaaacg cacgcgctag gttgccgcat ggtgaggccg
     5401 gcctcgtcgg ctcatggagc gcgccgcacg tgattttcac ccgccgaaac gcggggccaa
     5461 gtacgcagca gtgcacacca tcggacgcag tggctaatcg agtagccggc gctttgagtg
     5521 tgaattggcg tcgaaacccg cgagatgtca cccgtccggg tgtggtccgg tctttgtaag
     5581 gacttgggat ccatggcgat cgaatcgacc gctatcaacg acctgatcgc gggattccag
     5641 cagcgtccgc tgcagcggga ctcgaacgat tggttgttcg gcgagcgcga cgacgctacc
     5701 gagatcgata gcggtagcga ggagaaagcg acgacgccgt tcgatcgcag cacgccgcca
     5761 gtcgtcccac tgccgcaagc gttcgtgcat cagccggcgc ccgcgctcga ggcgctgtat
     5821 cgctcgccgt cgccgacggc gtacgtgcgc ccgatcagtg cgtcgggcgt cgcgaagaag
     5881 ctcgcgctgc cgatcggcgt gctgtc
//
LOCUS       DASUFX010000552        15865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1200060, whole genome shotgun sequence.
ACCESSION   DASUFX010000552 DASUFX010000000
VERSION     DASUFX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15865
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200060"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFV99_22020"
     CDS             complement(<1..1178)
                     /locus_tag="VFV99_22020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5062069.1"
                     /translation="MSLAERRWLRLFVLCALYVAQGVPWGFMFITLPTYLATRGVEDL
                     AMVMAMAYLPYAFKWIWGPIIDAFTIRRFGRRRPWIVFAQAMMALTVFAMLLFDVTRE
                     VKLLAAMIFVHTIFNSLQDVSVDALAVDLLPENERGRANGLMYGSKYLGGAMGGVGLA
                     YVISYSSLRTALVVQTSILLAIMMLPLFVRERDGEAPPREPLRDIAGALLQAFSLRST
                     LIAVLLTLGSTFAVGMIQPVAIKLFVSTLHWKLEDYGAISGGWGLVVGGVCAAITGFL
                     ADKLGRRRVAAVASCGLAVGWLVAAQMKPYWTEHWFVWVLGLYAEGCVAIWQVSLFAI
                     AMDLSWPRIAGSQFTAYMALMNLGTTLGTLFSTFAMEHFEFHGVYILAGCIQLAVTLL
                     L"
     gene            complement(1200..1922)
                     /locus_tag="VFV99_22025"
     CDS             complement(1200..1922)
                     /locus_tag="VFV99_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062070.1"
                     /translation="MPATTGVSAVPMNRPGGEVQLGPVPGFFLSSSAKGENKGAPIMQ
                     ASAMIEPDRWLHIPGLVLGGRLFGQSGDTPGEPYIGYRRKLRDDVAIGGGLYGTSKRS
                     TQKLASYHATRVGGEVALDARLLETEWFGFHLQGALAATHIDASGEYCVDMMGIAKDC
                     DIENPTMNTMIDGKQSGIYPAATGTLALDFAKGRDGVFDGARLALLIATGSMPMLKDG
                     EKTGSETYASLGLTLTLGIADE"
     gene            2066..3328
                     /locus_tag="VFV99_22030"
     CDS             2066..3328
                     /locus_tag="VFV99_22030"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5062071.1"
                     /translation="MTDKTKHKSKHIKLTSHPGAGGAKPIPIHWGAGDPKTRGPIIAT
                     LTNPDHRNVIGTHSGSYAVYRALAIAAKALDPQHVPDLTNTAPSEPFGPHPQWHDPER
                     IVSVDPYGHIVQDVFADYLKKGYDIRPTIAVTKAHIDMPEIVDAIEAGRIPIDGKIVK
                     NKREVPVTKAATEPVWYLPGVAKRFGCTESELRRTLFEQTGGMFPELVTRGDLDVFLP
                     PIGGQTIYIFGDPKNLSDPNVTLTARVHDECNGSDVFGSDICTCRPYLAHAIEECIRG
                     AQNGGVGLIVYFRKEGRALGEVTKFLVYNARKRQQGGDSAAQYFARTECVAGVQDMRF
                     QELMPDVLHWLGIKKIHRLVSMSNMKYDAIVRSGITVGERVKIPDELVPADAKVEMDA
                     KMAAGYFTPEAPPSAEELAKAKGRSLTE"
     gene            3467..3994
                     /locus_tag="VFV99_22035"
     CDS             3467..3994
                     /locus_tag="VFV99_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5062072.1"
                     /translation="MDNPSVDVDTQALDMPQGTKSDVTVSVDGAPVDSLFSVVWTIDD
                     PSIVSVTPEYDGKRLRVDAANPGETVVHVNSHGQTFDIPVRVGQPAITYIWIEPSTVT
                     TQVGSQVHVKATGLDTMYRLQDVTLDSYWAVRDENIANLDMAGMMLRAMGEGRTTLHA
                     NFGNLATITDVTILK"
     gene            4036..5307
                     /locus_tag="VFV99_22040"
     CDS             4036..5307
                     /locus_tag="VFV99_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HEX5062073.1"
                     /translation="MAASEAETAIGYLRSPLAIRARCENILEAAFSGELEHFAVEMTA
                     MPKVIDEVVAVTRANYPTLEIPVHGRVNHFRAGGVDRVAALDAKLQSLSRDERARAWI
                     DLVVVSVLLDAGAGDQWRFREASTGLEIGRSEGLAVASLHAFEQGLFSSDPQWPLRVD
                     AAGLKALEPERLAAAFQVSADNPLVGVDGRIGLVRALGNALTAAPMIFPGDRPAGLLD
                     HFLWIAIESRAPSGSMTRAVATKVDAPSILLALLEGLGPIWPGRIELGGVNLGDVWSH
                     PAAGGTGPSAGLVPFHKLSQWLAYSLFEPLTRAGLSITQPGALTGLPEYRNGGLFVDL
                     GVLVPKHARVREQVHAPGDEVIVEWRALTVALLDRVAEGVRERLGKSPGELPLANILE
                     GGTWAAGRAVAKRLRPGGGSPIQIKSDGTVF"
     gene            5457..5609
                     /locus_tag="VFV99_22045"
     CDS             5457..5609
                     /locus_tag="VFV99_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062074.1"
                     /translation="MTRLETIVTRQRKSRLRDLAFAAFVVLAGACSIATVSTAIHGAQ
                     HEVARR"
     gene            5697..6134
                     /locus_tag="VFV99_22050"
     CDS             5697..6134
                     /locus_tag="VFV99_22050"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HEX5062075.1"
                     /translation="MSGRRCLAYRVWLGEKNRPATETSETKAVSFLLEDDSGRALVEP
                     TDARIMISTDADARSGVLYAPMPAATEMLRRLELTASHTLLYKEAVISDGTTIAVLGT
                     CMREPDRDAPPADAYRGDAPTRLRVTSASSYPLVISDDPSALK"
     gene            6151..6780
                     /locus_tag="VFV99_22055"
     CDS             6151..6780
                     /locus_tag="VFV99_22055"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HEX5062076.1"
                     /translation="MWPLLLTVGVVGAGAVVFTLFTGNERIRRTLRNMPAKKIGELSD
                     DERAKIVGRVRALGEALEAPISGRRCVAYSVSVVEQEQNASHIRETKAVAFVVEDGSG
                     RALVDPTAARIAIDGGPSVQTGTLHDPTPAAEDLLLRHGKRPRHGVLYREAVITDGES
                     IAVLGAGTREPDPDAPPTEAYRGDARTRLRLTSSRKYPLVISDDPSALK"
     gene            6797..7468
                     /locus_tag="VFV99_22060"
     CDS             6797..7468
                     /locus_tag="VFV99_22060"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HEX5062077.1"
                     /translation="MSFAILALAIAGFAGWAFWHFGEDQTIRRTLRNAPTKRIQDLRD
                     DELGKVVGHARGLDEVLNAPLTGRRCVYFIATVEEHRSTGKSSYWKTIVRETRGVPFM
                     LEDGSGRALVDATAARITLDFDGKSQSGTFDDPTEAEKAFLARHGEKGEGWVFNRSLR
                     YREAVIEDGETIAVLGAGTREPDPDAPPTEAYRGDVPTRLRLTSSRKYPLVISDDPSA
                     TRVPR"
     gene            7699..7857
                     /locus_tag="VFV99_22065"
     CDS             7699..7857
                     /locus_tag="VFV99_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062078.1"
                     /translation="MNTRLENIATRQSKTRLRDAFFAACVALAAAVSITTVSTASHAA
                     SSAEVAQR"
     gene            7974..8606
                     /gene="upp"
                     /locus_tag="VFV99_22070"
     CDS             7974..8606
                     /gene="upp"
                     /locus_tag="VFV99_22070"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX5062079.1"
                     /translation="MPGKVNVIDHPLVQHKLTLMRSKETSTSTFRNLLREISMLLAYE
                     VCRDLPTAKVKIETPLASMTAPMLDGKKIVLISILRAGNGILDGMLEILPSARVGHIG
                     LYRDPKTLVAVEYYYKVPEAMADRDAIVVDPMLATGNSAVAAIDRLKESGPRSIKFVC
                     LLTCPEGIRVFHASHPDVPIYTAAVDDALNEKSYIVPGLGDAGDRLFGTK"
     gene            8944..9621
                     /locus_tag="VFV99_22075"
     CDS             8944..9621
                     /locus_tag="VFV99_22075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5062080.1"
                     /translation="MKKKASGNKVASQRVEKAARPDEAAPAAPPAAAASQENTDLAPV
                     VGANLRRLRTRRGLSLERLAQISGVSRAMLGQIELGQSAPTINVLWKIARALEVTFSA
                     LISARTQSGALVLRSQESKILTSKDRSFSSRALFPFDEPRRVEFYELRLSAGSVEDAD
                     AHPPGTTENLVVTAGTIEIDVAGDTHRLETGDSILFEADTPHAYRNAGKSEAVMYLVM
                     TYAEEIG"
     gene            complement(9618..10808)
                     /locus_tag="VFV99_22080"
     CDS             complement(9618..10808)
                     /locus_tag="VFV99_22080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062081.1"
                     /translation="MSGVDPLIGRNLDGRYTIADVIGRGGMGTVYRGLQHAVGRPVAI
                     KVVTPRPGADPSLFKRFLREAKLTSKLQHPNAVSIVDFGQTPDDLLYLVMEFVDGRTL
                     EETVAHGGKLDPARVVRIGTQICAALQGAHALQIIHRDLKPANIMLHSIAGQEMVKVL
                     DFGLAKSLGGENITANVTSAGDLLGTPQVIAPESVTGGIVDARVDLYSLGCTLYFAAM
                     GRLPFESKVIAELITMHAMEPPPPLTGVPVGLAAVIEKLLAKAPTDRYVDAAATSAAL
                     EGALAVGTEVVQSVAKPTPKGTMLGWVTPPSGTLRPTKPRSGETPVVRATPARVERSI
                     DTSTSSPAMIGTNLRPIDPRLAKAGVPQQPVAPSRGVPIAWAIIALVILASGGLVLYT
                     LLTR"
     gene            10830..11822
                     /locus_tag="VFV99_22085"
     CDS             10830..11822
                     /locus_tag="VFV99_22085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5062082.1"
                     /translation="MRSVVAQRYGGPEVLALVDRHEPVVDGRDVLIEVRAASLNPLDY
                     KVRAGKAKLALPLKPPIALGCDVAGVVSATGGDVTRFNVGDEVFARLEKLRMGGLAER
                     VAADESVVAKKPQRASFVEAAAVPLAALTALQALRGSAALQPGQRVLIHAGAGGVGTL
                     AIQIAKILGLHVVTTTSTKNIDFVKSLGADEVVDYTKERVTDRVKDLDAVFDTLGTTE
                     IESLGLVKRGGVVVGIGGLPDEAFAREWLPGWTRPAIWLMTAKRRRAAERAGARFVYL
                     FMRPDGAELAEIASWIDDGKLKPIVHETYPLAEFREAFAELERGRARGKIVLTI"
     gene            complement(11819..12664)
                     /locus_tag="VFV99_22090"
     CDS             complement(11819..12664)
                     /locus_tag="VFV99_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062083.1"
                     /translation="MSRASRAALVAVLLASSAAAAAPKGAAARAQFDKGVTAYTKGDY
                     AAASAALGSSFVLEPDPETLFAWAQTERKLGHCDRAIELYTKLLKMDLPAENKEAVKV
                     QIGECKDIIADEKKANATGPKPKIDAKPKTTEKKPAQTKVEPAPPPPVEPTPPPPVEQ
                     PIAQPPPPVESKPEGRTWWKDPVGGALVGAGAIGIGLGVVFLVQGRAADSDKANAMSY
                     PEYETLANRAESRGRLGVISLVAGGALAAGGVVWYVTRKPQAEKTVTTLVVPGGGGVA
                     FSGRF"
     gene            complement(12661..14709)
                     /locus_tag="VFV99_22095"
     CDS             complement(12661..14709)
                     /locus_tag="VFV99_22095"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062084.1"
                     /translation="MNAATQRTRHLGPGDKLGKYEVIRQIAVGGMAELYLARTFGIEG
                     FEKLVVLKRILPQYGTNASFVDMFLNEARLAATLHHPNIAQVYDIGLEEGDYFFAMEY
                     VHGEDLDHISIASEEQGVPLSMDAALTLVAGLCAGLHYAHEKLNAEGRPIGIVHRDVS
                     PSNVLVSYDGAVKLVDFGIARASSRPGTTQGGLKGKISYMSPEQCRAKAQLDRRSDLY
                     SVGSILYELTTGQLPFSGETEYQILNQIVNEDAPPPSTVVPSYPRHLERIVLKALARD
                     ADQRYATALQLQTDLEDFAHESRLRVSPLVLARIMGTLFPARLEEWHDAKKQGAFFVE
                     QHVVRTLIESGKTPDPNDPEGRAHVAALAAQAAADEAAARNFGDDDPTSLSQSFVDLN
                     DTTIGQPPISTDASGSFNRAREITELPTHGEGRMSWDQSSVNIPAAPEPKPPQAYPVV
                     ASLLTASPGSLVTSAGKYGGLPANEPTQIQVGDVTERVRVKRHSSPTAFVRNPSRKPM
                     FAMGAVVVIAGVLFAVIAMRGTAKTPAPQETVAPSVEMKVTPTAEPPQTTEQVGQEPT
                     NGAAQPAAESVNAESSGHAATPEPKKSEPETAEPVKKPELVKSEPKKPELVKTEPKAV
                     KTEPVKKTEAAKKTAAVKKAEPKKIEAVKKEPAKKAEAKKETTWNTDSPFMPVRTEKK
                     "
     gene            14905..>15865
                     /locus_tag="VFV99_22100"
     CDS             14905..>15865
                     /locus_tag="VFV99_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062085.1"
                     /translation="MDCTSGFRYGELSGPMSGTCVGVQQQDGGVDIDTPPGQVCYGTG
                     LVMACFAEAPTNPQTFTQNTTINTDTSLLCATTLNQTGACVIAAQSITINGGIYVAAV
                     GTKPLVFVADQTITVTGRIDVASYRQFMSVGAAGDVPGCNAGTAPTGTSGGAGGSFGG
                     LGGAGAAVGTAGMPGAVLTPTVLRGGCPGQTGADNVAPMGPGTGGHGGGAVFLIANTS
                     ISVAGTGVIDASGEGGAGATSSTSGGGGGGGSGGYIGFDTPSLMVSGAVFANGGGGGE
                     ASGASTTGVNGADPTLPTTAALGGAGGSNFGTDGGNGSVGATLN"
BASE COUNT         2744 a         5219 c         5184 g         2718 t
ORIGIN      
        1 aggagcagag tgaccgcaag ctggatgcat ccagcgagga tgtacacgcc gtgaaactcg
       61 aagtgctcca tcgcgaatgt cgagaacagc gtgccgagcg tggtgccgag attcatcagc
      121 gccatgtacg ccgtgaactg agagcccgcg attcgcggcc acgatagatc catcgcgatc
      181 gcgaacagcg atacctgcca gatcgcgacg caaccttcgg cgtacagacc gagcacccag
      241 acgaaccagt gctcggtcca atacggcttc atctgtgccg caacgagcca acccaccgcg
      301 agtccgcacg acgcgaccgc ggcgacacgt cgtcgaccga gcttgtcggc gaggaacccg
      361 gtgatcgcgg cgcacacgcc gccgacgacg agcccccagc cgccgctgat cgcgccgtag
      421 tcttccagct tccagtgcag cgtactgacg aacagcttga tggcgaccgg ttggatcatg
      481 ccgaccgcga acgtcgagcc caacgtcagc agaactgcga taagtgtcga gcgcagcgag
      541 aacgcctgca gcagcgcacc ggcgatgtcg cgcaatggtt cgcgtggcgg agcctcgcca
      601 tcgcgctcgc gcacgaacag cggcaacatc atgatcgcga gaagaatcga ggtctgaacc
      661 acgagcgccg tgcgcagcga cgagtacgag atgacatatg cgaggccgac gccacccatc
      721 gcgccgccga ggtacttcga gccgtacatc agcccgttgg cacgcccgcg ctcgttttcc
      781 ggcagcagat cgacggcaag cgcgtcgacc gagacgtcct gcagcgagtt gaagatcgtg
      841 tgcacgaaga tcatcgccgc gagcagcttc acctcgcggg tgacatcgaa cagcagcatc
      901 gcgaagacgg tgagcgccat catcgcctgc gcgaacacga tccacggccg gcgccggcca
      961 aaccgccgga tcgtgaacgc gtcgatgatc gggccccaga tccacttgaa cgcgtacggc
     1021 aggtaagcca tcgccatcac catggcgagg tcctcgacac cacgtgtcgc gagatacgtg
     1081 gggagcgtga tgaacatgaa gccccacggc acgccctgcg cgacatagag cgcgcacagc
     1141 acgaacagcc gtagccagcg tcgctccgcg agactcacgc gcggagccta acatcgcggt
     1201 tactcgtccg cgattccgag cgtcaacgtg agaccgagcg atgcgtaggt ttctgagccg
     1261 gtcttttcgc cgtccttcag catcggcatc gagccggtgg cgatcaagag cgcgaggcgc
     1321 gcgccatcga acacgccgtc gcgtcccttc gcgaagtcga gcgcgagcgt gccggtcgcc
     1381 gcgggataga tgcccgactg tttgccgtcg atcatcgtgt tcatcgttgg attctcgatg
     1441 tcgcagtcct tcgcgatgcc catcatgtcg acgcagtact cgccactcgc atcgatgtgc
     1501 gtcgccgcga gtgcgccctg caaatgaaag ccgaaccact cggtctcgag cagccgcgcg
     1561 tcgagcgcca cctcaccacc gacgcgggtc gcgtgatacg aggcgagctt ctgcgtcgag
     1621 cgcttgctcg taccgtagag gccgccaccg atcgcgacgt cgtcgcgcag tttgcggcga
     1681 tacccgatgt acggctcgcc gggcgtgtcg ccgctctggc cgaacagccg tccgccgagt
     1741 acgaggccgg ggatgtggag ccagcgatcg ggctcgatca tcgccgacgc ctgcatgatg
     1801 ggcgcgccct tgttctcgcc ctttgccgac gagcttagaa agaaccccgg taccgggccg
     1861 agctgcacct cgccgccagg tcggttcatc ggcaccgccg acacacctgt ggtcgccggc
     1921 atggggccta acgtggtgca acccgtgatc aaaacaagaa ttgcgatccg catgaatcga
     1981 caacacgcga gccaggcatc ttgccacccc ggaaagcccg gcagtggacg gcacctacat
     2041 tcgggtctag gctcgtcaac ttcccgtgac ggacaagacc aagcacaagt ccaagcacat
     2101 caagctcacg tcgcatcctg gcgcgggcgg cgcgaagccg attcccatcc attggggtgc
     2161 gggcgatccg aagacgcgcg gtccgatcat cgcgacgctc accaatccag atcatcgcaa
     2221 cgtgatcggc acgcactcgg gcagctacgc ggtgtatcgc gcgctcgcga tcgcggcgaa
     2281 ggcactcgac ccgcagcacg ttcccgatct gacgaacacc gcgccgagcg agccgttcgg
     2341 tccgcatccg cagtggcacg atcccgagcg aatcgtttcg gtcgatccat acggccacat
     2401 cgtccaggac gtgttcgccg actatctgaa gaagggctac gacattcgcc cgacgatcgc
     2461 ggtcacgaag gcgcacatcg acatgcccga gatcgtcgac gcgatcgagg ccggccgcat
     2521 tccgatcgac ggcaagatcg taaagaacaa gcgcgaggtg ccggtcacga aggccgcgac
     2581 cgagccggtg tggtatttgc ccggcgtcgc gaagcggttc ggctgcaccg aaagcgaatt
     2641 gcgccgcacg ctgttcgagc aaaccggcgg catgttcccc gagctcgtca cgcgcggtga
     2701 cctcgacgtg ttcctgccgc cgatcggcgg ccagacgatc tacatcttcg gcgatccgaa
     2761 gaatctctcc gatccgaacg tgacgctgac ggcgcgcgtg cacgacgagt gcaacggctc
     2821 cgacgtgttc ggctccgata tctgcacgtg ccgtccgtac ctcgcgcacg cgatcgaaga
     2881 gtgcatccgc ggtgcgcaaa acggtggtgt cgggctcatc gtctacttcc gcaaggaagg
     2941 tcgcgcgctc ggcgaagtca cgaagttcct cgtctacaac gcccgcaagc gccagcaagg
     3001 cggcgacagc gcggcgcagt acttcgcgcg caccgagtgc gtcgccggtg tccaagacat
     3061 gcgctttcag gagctgatgc ccgacgtgtt gcactggctc ggcatcaaga agatccaccg
     3121 cctcgtgtcg atgtcgaaca tgaagtacga cgcgatcgtg cgcagcggca tcacggtcgg
     3181 cgaacgcgtg aagatccccg acgagctcgt ccctgccgac gcgaaggtcg agatggacgc
     3241 gaagatggct gccggctact tcacgccgga agcgccgccg agcgccgaag agctcgcgaa
     3301 ggccaagggc cgcagcctca ccgagtagcg cgcgcttcga taagcggcgc gtaatcgcgg
     3361 gccgtcagtc gatgcgttgg ctgttttctt ggccccgtcg catggcctcg gttagctgtg
     3421 gaccatgcga cgactcggat tctgtttatt cgtgctggcg gggtgcttgg acaatcccag
     3481 tgtcgatgtc gatacccagg cgctcgacat gccgcaaggc accaagagcg acgtcaccgt
     3541 gtccgtcgat ggggcaccgg tcgacagcct gttcagcgtc gtgtggacga tcgacgatcc
     3601 atcgatcgtc tcggtgactc ccgagtacga cggcaagcgg ctccgcgtcg atgcagctaa
     3661 cccgggcgag acagtggtcc acgtcaactc gcatggccag acgttcgaca ttccggtccg
     3721 cgtcggtcag cccgcaatca cgtacatctg gattgaaccg tcgacagtta cgacccaggt
     3781 cggctcgcag gttcacgtca aagccacggg actcgacacg atgtaccggc tccaggatgt
     3841 aacgctcgac agctactggg cggtccggga cgagaacatc gcgaacctcg acatggccgg
     3901 catgatgctg cgggcgatgg gcgagggacg cacgacgctg cacgcgaact tcggcaacct
     3961 cgcgacgatt accgacgtca cgatcctgaa gtgacctagc gctactcgcg cggctccggt
     4021 agcttgatgc tgtagatggc agcgagtgaa gccgagaccg cgatcgggta tctccggtct
     4081 ccactcgcga tccgcgcgcg ctgcgagaac attctcgaag cggcgttctc gggcgagctc
     4141 gagcacttcg cggtcgagat gactgcgatg ccgaaggtga tcgacgaagt cgtcgcggtc
     4201 acgcgggcga attacccgac gctcgagatt ccggtgcacg gccgcgtcaa tcacttccgt
     4261 gccggcggtg tcgatcgcgt cgcggcgctc gacgcgaagc tgcagtcgct gtcgcgcgac
     4321 gagcgcgcgc gcgcgtggat cgatctcgtc gtcgtctcgg tgctgctcga tgcaggcgcc
     4381 ggcgatcagt ggcgctttcg cgaagcgtcg accgggctcg agatcggtcg ctccgaaggc
     4441 ctcgcggtcg cgagcctgca cgcgttcgag caaggtttgt tctcgtcgga tccgcagtgg
     4501 ccgttgcgcg tcgatgcagc gggcctgaag gcgctcgagc cggagcggct cgccgcggcg
     4561 ttccaggtca gcgcggacaa tccactcgtc ggtgtggacg gccgcatcgg cctcgtgcgc
     4621 gcgctcggca acgcgctcac cgccgcgccg atgatcttcc caggcgatcg gccagccggc
     4681 ctgctcgatc atttcctgtg gatcgcgatc gagagtcgcg caccgtcggg gtcgatgact
     4741 cgtgcggtcg cgacaaaagt cgatgcgccg tcgatcttgc tcgcgctcct cgaaggcctc
     4801 ggtccgatct ggcccggtcg catcgagctc ggtggcgtca atctcggcga cgtgtggtcg
     4861 catcccgcgg cgggcggcac cggtccgtcg gccgggctcg tgccgttcca caaactttcg
     4921 cagtggctcg cgtactcgct gttcgagccg ctcacgcgcg ccggtctctc gatcacgcag
     4981 ccgggcgcgc tcacgggctt gcccgagtat cgcaacggcg gcttgttcgt cgatctcggc
     5041 gtgctggtgc cgaagcatgc gcgggtgcgc gagcaggttc acgcgcccgg cgacgaagtg
     5101 atcgtcgagt ggcgcgcact cacggtcgcg ctgctcgatc gcgtcgccga aggtgtgcgc
     5161 gaacggctcg gcaaatctcc cggcgagctt ccgctcgcaa acattctcga aggcggcacg
     5221 tgggccgcgg gccgtgcagt cgccaagcgc ctacgtcccg gcggcggctc gccgatccag
     5281 atcaagtccg acggcacggt gttctaacca cccgttcagc ggagcgacgg tctctgcgac
     5341 acctggtgcg acagtcgcgg atcctttgac gctgcgctaa gtggccgcac ctgttcgcgt
     5401 cagtcatggc atcgcagttg aagaagggat gtcccgccga aacaaggagc cccgccatga
     5461 cccgcctcga aaccatcgtc actcgccaac ggaagtctcg tctgcgtgac ctcgcgttcg
     5521 ccgcgttcgt cgtgctcgcc ggtgcgtgct cgatcgcgac ggtctcgacg gcaatccacg
     5581 gcgctcagca cgaagtcgcg cgccgctgat acgctagccg atgtggccgc tcgtggtcat
     5641 ggtcggcgaa gatcgtcggt cgcgtacgcg gcgtcggcga accgctcgag gccccgatta
     5701 gcggccgccg ctgccttgcg taccgcgttt ggctcggcga gaagaaccgc ccggccacag
     5761 agacgagcga gacgaaggca gtctccttct tactcgaaga tgacagtggt cgtgcgctcg
     5821 tcgagccaac cgatgcgcgc atcatgatca gcacggacgc tgatgcgcga agcggggtgc
     5881 tctacgcacc gatgcccgcc gcgacggaaa tgctgcgccg gctcgaactg acagcgagcc
     5941 acacgctcct ctacaaggaa gcggtgatca gcgacggcac gacgatcgcc gtgctcggaa
     6001 cgtgcatgcg cgagcccgac cgcgatgcgc cgcccgcgga cgcctaccgt ggcgacgcgc
     6061 cgacgcggct gcgtgtcacg agcgcttcga gctacccgct cgtgatcagc gacgacccct
     6121 cggcgctgaa ataggcgcta cgctcggagc gtgtggcccc tgctgctcac ggtcggcgtc
     6181 gtcggcgcgg gagccgttgt cttcacgttg ttcacgggaa acgaacgcat ccgccgcacg
     6241 ctgcgcaaca tgccggccaa gaagatcggc gagctgagtg acgacgaacg cgccaagatc
     6301 gtcggccgcg tgcgcgccct gggcgaagcg ctggaagcgc cgatcagtgg gcgacgctgt
     6361 gtcgcgtaca gcgtcagtgt cgtcgaacaa gagcagaacg cctcgcacat tcgcgagacg
     6421 aaagcggtcg cgttcgtcgt cgaggacggc agcgggcgag cgctcgtcga tccgaccgcc
     6481 gcacgcattg cgatcgatgg cgggcccagc gtgcaaaccg gcacgcttca cgatccgacg
     6541 ccggcggccg aggatctgtt gctccgacac ggtaagcgac cacgtcacgg cgtgctctat
     6601 cgcgaggccg tgatcaccga cggcgaatcg atcgcggtgc tcggcgccgg cacgcgcgag
     6661 cctgatcccg atgcaccgcc gaccgaggca tatcgtggtg acgcgcgaac gcgactgcgg
     6721 ctgaccagct cgcgaaagta cccgctcgtg atcagcgacg acccatcggc gttgaaatag
     6781 gcgctacgct cactgcgtgt cgttcgcgat cctcgctctt gccatcgcag gctttgcggg
     6841 ctgggcgttc tggcacttcg gggaagacca gacgattcgc agaacgctgc gcaacgcgcc
     6901 gacgaagcga atccaggatc tgcgcgacga cgagctcggc aaggtcgtcg gtcacgcgcg
     6961 aggtctcgac gaggtgctca acgcgccgct caccgggcga cggtgcgtgt atttcatcgc
     7021 gaccgtcgag gagcatcgct cgacgggcaa gagctcgtac tggaagacaa tcgttcgcga
     7081 gacgcgcggc gtaccgttca tgctcgaaga tggcagcggc cgcgcactgg tcgacgcgac
     7141 cgcggcacgc atcaccctcg acttcgatgg caagagtcag tcgggcacgt tcgacgatcc
     7201 gaccgaagcg gagaaagcgt ttctcgcgcg tcacggcgag aaaggcgaag gctgggtctt
     7261 caatcgctcg ctgcgctatc gcgaagcggt gatcgaagac ggcgaaacga tcgcggtgct
     7321 cggcgccggc acgcgcgagc ccgatccgga tgcgccgccg accgaggcgt atcgcggcga
     7381 cgtgccgaca cgacttcggc tcaccagctc gcgtaagtac ccactcgtga tcagcgacga
     7441 tccgtccgcc acgcgggttc cgcgctaact cacgccactc accgcgctaa ctctgcggcg
     7501 cgccgtaccg gggttcgttt cgcggcaccg taccggggtt cgtttcgcgg cgcggagcct
     7561 gcgacagcgg caggactgcg acattcggtg cgacagggca aagctggttc gcccgcggtt
     7621 aagtccgtga aatggtgcgg agaaaacatg gcatcgcggt cgcaataggt ccgttcgctt
     7681 ctgccgaaag gatctcccat gaacacccgc ctcgaaaaca tcgccacccg ccagagcaag
     7741 acccgccttc gtgacgcgtt cttcgccgcg tgcgtggcac tcgccgccgc ggtttcgatc
     7801 acgacggtgt cgaccgcgtc gcacgccgcg tcgtctgccg aggtcgcgca gcgctaatgc
     7861 cgcccgagct gccgcgcgag cttcgcgcac gtagctcgtc gcgagtcatg cgctacctgt
     7921 cgtccgcgcc gcgtggctca tcagcgatcg ggtctgatac ctttccggtg cacatgcccg
     7981 gcaaggtcaa cgtcatcgat catccgctgg tccaacacaa gctgacgctg atgcgctcga
     8041 aagagaccag caccagcacg ttccgcaacc tgcttcgcga aatctcgatg ctcctggcct
     8101 acgaagtgtg ccgggacctg ccgacggcga aagtgaagat cgagacgccg ctagcctcga
     8161 tgacggcgcc gatgctcgac ggcaagaaga tcgtgctgat ctcgatcctg cgcgccggca
     8221 acggcatcct cgacggcatg ctcgagatct tgccgtccgc gcgtgttggc cacatcggcc
     8281 tctaccgcga ccccaagacg ctcgtcgcgg tcgagtacta ctacaaggtt cccgaggcga
     8341 tggccgatcg cgacgcgatc gttgtcgacc cgatgctcgc gaccggcaac tccgcggtcg
     8401 ctgcgatcga tcgcctcaaa gagagcggcc cacgctcgat caagttcgtc tgtttgctca
     8461 cgtgcccgga aggcattcgc gtgttccacg cgagtcatcc ggacgtcccg atctacacgg
     8521 cagcagtgga cgatgcgctc aacgagaaga gctacatcgt cccgggtctt ggcgacgccg
     8581 gcgatcgcct gttcggtacc aagtagcgtc cgcgaggcgg acgcgaaaac taaccccggt
     8641 acgttggagc ggtcgcgatg cgggccgcaa agtaaccccg gtacgtaatc ggcctcaccc
     8701 aataagtttg gtcattactc acggttggca gtttgtccgt aaaaacagat tgaacgtttc
     8761 cgctcaaacg gataaatttc cgtacatgag caacgcagct gtcgccaaca acatcgtgag
     8821 cctcgacgag taccgtcgta atcgcggtga gcggcagccg atgcaggctc gcagccagac
     8881 cgttactcgg ttgaccccgg ccacaccggc cgtgtgggta tactgggtcc cggtttgggt
     8941 ctggtgaaga agaaagcgtc aggaaacaaa gtggcaagcc agcgggtgga gaaggctgcg
     9001 cgtcccgacg aagcagcacc tgctgctcct ccggccgcgg cagcgtcgca agagaacacc
     9061 gatctcgcgc cggtcgtcgg tgccaacctc cgtcgcctgc gcacgcgtcg cgggctctcg
     9121 ctcgagcggc tcgcgcagat ctccggcgtg tcgcgcgcga tgctcggcca gatcgagctc
     9181 ggccagagtg cgccgaccat caacgtcctg tggaagatcg ctcgcgcgct cgaggtcacg
     9241 ttctccgcgc tgatcagcgc gcgtacgcaa tcaggcgcgc tcgtgttgcg ctcgcaggaa
     9301 tcgaagatcc tgaccagcaa ggaccgtagc ttctcgtcgc gcgcactgtt cccgttcgac
     9361 gaaccgcgcc gcgtcgagtt ctacgagctg cgcctttcgg ccggcagcgt cgaggatgcc
     9421 gatgcgcacc cgcccggtac gaccgagaac ctcgtcgtca ccgcgggcac gatcgagatc
     9481 gatgttgcgg gcgacacgca ccggctcgag accggtgatt cgattctgtt cgaggccgac
     9541 acgccgcacg cctaccgcaa tgcgggcaag agcgaagccg tgatgtacct cgtgatgacg
     9601 tacgccgaag aaatcggcta acgcgtcagc agcgtgtaga gcacgaggcc gccgctcgcg
     9661 agaatcacga gcgcgatgat ggcccacgcg atcggcacac cgcgcgatgg cgcgaccggt
     9721 tgctgcggca cgcctgcctt cgctagccgc ggatcgatcg gcctcaggtt cgtcccgatc
     9781 atcgcgggcg agctcgtcga ggtatcgatc gaccgctcga cgcgcgctgg cgtcgcacgc
     9841 acgaccggtg tttcgccact gcgcggcttc gtcgggcgga gtgttcccga cggtggggtg
     9901 acccagccga gcatggttcc cttcggtgtc ggcttcgcca cgctttgcac gacctcggta
     9961 ccgacggcga gcgcgccttc gagcgcagcg ctcgtcgcgg cggcatcgac gtagcgatcg
    10021 gttggcgctt tcgcgagcag cttctcgatc acggctgcga ggccgaccgg cacacccgtg
    10081 agcggcggcg gcggctccat cgcgtgcatc gtgatcagct ccgcgatcac cttcgattcg
    10141 aacggcaacc tgcccatcgc cgcgaagtac agcgtgcagc cgagcgagta gagatcgacg
    10201 cgcgcatcga cgatgccacc cgtcaccgac tcgggcgcga tcacctgcgg cgtgccgagc
    10261 agatcgcccg cgctcgtcac gttcgcggtg atgttctcgc caccgagcga tttcgcgagc
    10321 ccgaagtcga ggaccttcac catctcctgg ccggcgatcg agtggagcat gatgttcgcc
    10381 ggcttcagat cgcgatgaat gatctgcagc gcgtgcgcgc cctgaagcgc ggcacagatc
    10441 tgcgtgccga tgcgaacgac gcgtgccggg tcgagcttgc caccatgggc gacggtttct
    10501 tcgagcgtgc gaccgtcgac gaactccatc acgaggtaca acagatcatc gggcgtctgg
    10561 ccgaagtcga cgatcgagac cgcgttcgga tgctggagct tgctcgtgag cttcgcttcg
    10621 cgcaggaagc gcttgaacag cgacggatcg gcgcccgggc gtggcgtcac gaccttgatc
    10681 gcaacgggac gacctaccgc gtgctgcaat ccgcgataga ccgtgcccat accgccgcgg
    10741 ccgatgacat cggcaatcgt gtaccgacca tcgagattgc ggccgatcag cggatcgaca
    10801 cccgacatcc gaagtgcgag cgtaacacca tgcgaagcgt agtcgcacag cgttatggcg
    10861 gtcccgaggt cctcgcgctc gtcgaccgac acgaaccggt tgtcgacgga cgcgacgtgc
    10921 tgatcgaggt tcgcgcagcg agcttgaacc cactcgacta caaggtccgt gcgggcaaag
    10981 caaagctcgc actgccgctg aaaccgccga tcgcgctcgg ctgcgacgtc gccggtgtgg
    11041 tgagcgcgac cggtggcgat gtcacgcgct tcaacgttgg cgacgaagtg tttgcgcggc
    11101 tcgaaaaact tcggatgggc ggcctcgccg agcgcgtcgc tgccgacgag agcgtcgtcg
    11161 cgaagaagcc gcaacgcgcg agcttcgtcg aggcagccgc ggtcccactt gcagctctga
    11221 cggcgctgca ggcattgcgc gggtcggcgg cattgcagcc cggccagcgc gtgttgatcc
    11281 atgctggtgc gggcggcgtc ggtacgctcg cgatccagat cgcgaagatc ctcggtctcc
    11341 atgtggtcac gacgacgagc acgaagaaca tcgacttcgt gaagagcctc ggcgccgacg
    11401 aggttgtcga ctacacgaag gagcgggtga ccgatcgcgt gaaggatctc gacgcggtgt
    11461 tcgacacgct cgggactacc gagatcgaat cactcggcct cgtgaagcgg ggcggtgtcg
    11521 tcgtcgggat cggcggcttg cccgacgagg cgttcgcgcg cgagtggctg ccggggtgga
    11581 cgcgacccgc gatctggctg atgacggcga agcgtcggcg cgccgctgag cgagccggcg
    11641 cgcggttcgt ctatttgttc atgcggcccg acggcgcaga gctcgccgag atcgcgagct
    11701 ggatcgacga cggcaagctg aagccgatcg tgcacgagac gtatccgctc gcggaattcc
    11761 gcgaggcgtt cgccgagctc gagcgcggcc gcgcacgcgg caagatcgtg ctgacgatct
    11821 agaagcgtcc cgagaacgca acgccgccgc cgcccgggac gacgagcgtc gtgacggtct
    11881 tctccgcctg tggcttccgc gtgacgtacc agacgacccc gcccgctgcg agcgcaccgc
    11941 cggcgacgag actgatcacg ccgagccgac ctcgcgactc cgcgcgattc gcaagcgttt
    12001 cgtactcggg atacgacatc gcgttcgctt tgtcagagtc ggcagcgcgg ccctgcacga
    12061 gaaacacgac gccgaggccg atgccgatcg cgccggcgcc gacgagcgca ccaccgaccg
    12121 gatccttcca ccacgtgcgg ccttccggtt tcgactcgac aggcggtggc ggctgcgcga
    12181 tcggctgctc cacgggtggt ggaggcgttg gctcgaccgg tggcggcggc gcgggctcga
    12241 ccttcgtttg cgcaggcttc ttctcggtcg tcttcggctt ggcatcgatc ttcggcttcg
    12301 gcccagtcgc gtttgctttc ttctcgtcgg caatgatgtc cttgcactcg ccgatctgga
    12361 ccttcactgc ttccttgttc tcggccggta gatccatctt gagcagcttc gtatagagct
    12421 cgatcgcacg atcgcaatga cccagcttgc gctcggtttg cgcccacgca aacagcgtct
    12481 ccggatccgg ctcgagcacg aacgacgagc cgagcgccgc ggacgccgcc gcgtagtcac
    12541 ccttcgtgta cgcggtgaca cccttgtcga actgtgcacg tgccgcggca cccttcggcg
    12601 cagcagctgc tgcactgctc gcgagcagca cggcgaccaa cgcggcacgc gatgcgcgac
    12661 tcatttcttc tccgtgcgca cgggcatgaa cggcgagtcg gtgttccacg tcgtctcctt
    12721 cttcgcctcg gccttctttg ccggttcctt cttcaccgct tcgatcttct tcggctccgc
    12781 cttcttcact gccgcggtct tcttggccgc ctcggtcttc ttcacgggct ctgtcttcac
    12841 ggccttcggt tcggttttca cgagctctgg cttcttcggc tcggacttca cgagctccgg
    12901 cttcttgacc ggctctgccg tctcgggctc ggacttcttc ggttcgggcg tcgcagcgtg
    12961 ccccgaggac tcggcgttca ccgactcggc agcgggctgt gctgcgccgt tcgtcggttc
    13021 ctgccccacc tgctcggtgg tttgcggcgg ctcggcggtt ggcgtgacct tcatctcgac
    13081 ggacggtgca acggtttcct gcggagccgg cgtcttcgcg gttccccgca tggcgatcac
    13141 ggcgaacagt acgccggcga tcacgacaac cgcgcccatc gcgaacatcg gtttccgcga
    13201 cgggttgcgg acgaacgcgg tcggtgacga gtgacgcttg acgcgcacgc gctcggtgac
    13261 gtcgccgact tggatctgcg tgggctcgtt cgcaggcagc ccgccgtatt tgccggcgga
    13321 cgtgacgagt gagcctggcg atgccgtcaa cagcgaggcg acgaccggat acgcctgcgg
    13381 cggtttaggc tcgggtgcag ccgggatgtt caccgacgac tgatcccacg acatccgacc
    13441 ttcgccgtgt gtcggtagct cggtgatctc tcgcgcacga ttgaacgaac cgctcgcgtc
    13501 ggtcgagatc ggtggctggc cgatcgtcgt gtcgttgagg tcgacgaatg actggctgag
    13561 cgaggtcgga tcgtcgtcgc cgaaattgcg tgccgccgct tcgtcggcag cggcctgtgc
    13621 agcgagcgcg gcgacgtgcg cccggccttc cggatcgttt ggatcgggcg tcttgccact
    13681 ctcgatcagc gtacgcacga cgtgctgctc gacgaagaac gcgccctgct tcttcgcgtc
    13741 gtgccactcc tcgaggcgcg cggggaacag cgtgcccatg atgcgcgcga gcacgagcgg
    13801 tgacacgcgc aaccgcgact cgtgcgcgaa gtcttcgagg tcggtctgca gctgtagcgc
    13861 cgtcgcgtac cgttgatcgg cgtcgcgcgc gagcgccttg agcacgatac gctcgaggtg
    13921 ccgcggatag ctgggaacaa ccgtgctcgg cggcggcgca tcctcgttca cgatctgatt
    13981 gaggatctgg tactcggtct cgcccgagaa cggcagttga ccggtggtga gctcgtagag
    14041 aatcgagccc acggagtaga gatcgctgcg gcgatcgagc tgtgccttcg cgcggcactg
    14101 ctcgggagac atgtacgaga tcttgccctt gaggccgcct tgcgtcgtgc ccggccgcga
    14161 cgacgcgcgc gcgatgccga agtcgacgag cttgaccgca ccgtcgtagc tgacgagcac
    14221 gttcgacggc gatacgtcgc gatgcacgat gccgatcggc ctgccttcgg cgttcagctt
    14281 ctcgtgcgcg tagtgcagac cggcgcacag accggcgacg agtgtgagcg cggcgtccat
    14341 cgacaacgga acgccctgct cctccgacgc tatcgaaatg tgatcgaggt cttcgccgtg
    14401 gacgtactcc atcgcgaaga agtagtcgcc ctcctcgagg ccgatgtcgt agacctgcgc
    14461 gatgttcggg tgatgaagcg tcgcggcgag gcgcgcttcg ttgaggaaca tgtccacgaa
    14521 gctcgcgttg gtgccgtatt gcggaaggat gcgcttcagc acgacgagct tctcgaagcc
    14581 ttcgatgccg aacgtccgcg cgagatagag ctccgccata ccgccaacgg cgatttgtcg
    14641 gatcacctcg tacttgccga gcttgtcgcc aggtccgagg tgccgcgtac gttgtgtcgc
    14701 cgcgttcacc ccactacaac gatagacgaa aagccccgtt actgcagtac aatcacccca
    14761 atggcgcggg tagccgtcgt ggcgtgcgca atggggcttt ggtgcgggtg tagcccgtac
    14821 ggaggcggtg aatttgcgtg tacgaccgat tcggagtgtg gcggcgatgg ccagtgcagc
    14881 gccgggtttt gtagttttcc agatatggac tgcacgtccg ggtttcgcta cggcgagctc
    14941 tccgggccaa tgtccggtac gtgcgtcggc gtgcagcagc aagacggcgg cgtcgacatc
    15001 gatacgccgc ccggccaggt ctgctatggg accgggctcg tcatggcgtg cttcgcggaa
    15061 gccccgacga acccgcaaac gttcacgcag aacaccacaa tcaacaccga cacctcgctc
    15121 ctttgcgcga cgacgctcaa tcagaccggt gcgtgtgtga tcgcagcgca gtcgatcacg
    15181 atcaacggcg gcatctacgt cgcggccgtc ggcacgaagc cgctcgtgtt cgttgcggat
    15241 caaacgatca ccgtcaccgg ccgcatcgac gtggcgagct atcgccagtt catgtcggtc
    15301 ggtgcagcag gtgatgttcc cggatgcaac gcgggcactg caccgactgg aacctcgggt
    15361 ggcgccggcg gcagcttcgg cggactcggt ggtgcgggcg cggcggtcgg gaccgcaggc
    15421 atgcctggcg ctgtgctcac gccgacagtg ttgcgcggcg gctgtcccgg gcaaaccggc
    15481 gccgataacg tcgcgccgat gggcccgggc acgggtggcc acggcggcgg cgcggtgttc
    15541 ttgatcgcga atacatcgat cagcgtggcc ggcaccggtg tgatcgatgc gtcgggtgaa
    15601 ggcggagcgg gcgccacgag ctcgacatct ggcggtggcg gtggtggtgg ctcgggcggc
    15661 tacatcggct tcgacacgcc gtcgctgatg gtgagcggcg ccgtgttcgc gaatggcggc
    15721 ggcggcggtg aagcgagtgg tgcgtcgacc actggcgtca acggcgccga cccgacgttg
    15781 ccgacaactg ctgcactcgg cggcgctggc ggctcgaact tcggcaccga cggcggcaac
    15841 ggctcggtgg gcgcgacgct caatg
//
LOCUS       DASUFX010000553        21040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_783574,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000553 DASUFX010000000
VERSION     DASUFX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21040
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783574"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VFV99_22105"
     CDS             complement(<1..1267)
                     /locus_tag="VFV99_22105"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX5062086.1"
                     /translation="MSDDLDASLAMHDELIARRGVEIWIGAEPTFTRPESLDPAWSSN
                     AEGSDKLARAHALATAFADRTPGASVARVIGRQFPDETQPRFAFGVRWREDNIAATNG
                     ERTTADAVAVPPPTDLGADRWLSVTPDPGVVEVNMAPCATTRELYAQAQRIWTAAGAA
                     GLSATRFRFNGDLADSGGGGQLTLGGSRPDSSPFMRYPHVLPALVRYLNNHPSLSYWF
                     ASECVGAASQGPRPDEGTRERSDELAVALGWLEHLADRGELPPEQLQQGLGPLLVDSA
                     GNSHRAELNVEKLWNPHISVHGPRHGRMGVVELRAIRMPERPGMLAALAVLFRSIVAR
                     LVVNHYRAPLVDWHDELHDRFALPLALHRDLRIVLGDLDEHGLGIPAELRRELEAWRA
                     PGITCRLGDATLALRPALEFWPLVGDVASQ"
     gene            complement(1264..4557)
                     /locus_tag="VFV99_22110"
     CDS             complement(1264..4557)
                     /locus_tag="VFV99_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX5062087.1"
                     /translation="MRVLLQHRSRYVYPRPALLGPQLIRLRPADHARARIESYRLDVA
                     PEHRLHWQRDPHGNRVARVTFKAGQPVTELDIVVELAVDVHPVNPFDFFVDDRAKTIP
                     FQYPDDLAAELGVFLDVTDPAYRLGRLAVELLEKVPATGDTVGLLVEVNRLVKERVAY
                     VIRDEAGVWTPEETLQNGRGSCRDSAVLLVALLRARGIAARFASGYLIQLTDEGMIPN
                     EPKGVSRDVVDLHAWAEAYLPGAGWIGLDGTSGLLTGEGHIPLACTASPAHASPLDGT
                     SNVGASAVEFSTAIARLGHETRPTAPFTDEVWSDLLAAGDRADAALGAAGLNVTVGGE
                     PTFTARENVAAAEWQGGALGPDKWKRGRALADELLDRLAPGGLVLHRQGKQYPGESMP
                     RWALDVIARRDGVAMWPQRELRDCIGADDARRFGEQLAAALGVSTELHGAFEDPWEVV
                     RGEAQLPPDIDPRKAGLDDPEERRRLAKIFGQGVGSVVGWVLPLARSAQGSWLTERWS
                     FRRGELFLLPGDSPVGLRLPLGSLAASPAPPLWEEPDVDDPRRNPDDADEKAQAAERR
                     STPKRIARPGEPGIGIRTALAIEPRDGELWLFLPPVGRFEDFCALINAIDRARAATGF
                     TVNLEGYGPPPSPNLRRFGVTPDPGVLEVNLPPTYSAREAADVHATVFDAALASGLTA
                     ERYMLDGRMAGSGGGNHITIGGPTPLASPWLVRPDTLASLITFAQHHPSLSYMFTGLF
                     VGPTSQAPRVDEARHDALYELEVALPHLFDLQNAPPTWQIDAVLRHLLVDVAGSTHRA
                     EISIDKLFDPLTPHGRQGLVELRAFEMPPHPRMAAAQVVLTRGLLASFVKQPYKHALV
                     RWGAELHDRFLLPYYMWRDFEDVLAHLAAHDVALPADAYRPFVDLRCPLVGALETGGA
                     RVEVRNAIEPWHVLGEEATQTGTARYVDSSMERLEIRSHGLDEERYVITINGVTLPMR
                     RASGGRDVRVGGVRFRAWCPPHALHPHLGIHHPVRVDIIDTWAKRGVAGGTYHVWHPE
                     GRAYDAPPLTRVEASARRAQRWTLEGPAPWPVRARTAEPHPEQPYTLDLRRLDAGKPM
                     PRAEEWITE"
     gene            complement(4636..4983)
                     /locus_tag="VFV99_22115"
     CDS             complement(4636..4983)
                     /locus_tag="VFV99_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062088.1"
                     /translation="MSGVISQKLENSVDATRGQPLAMRVLARKDELEDALAELGPYDI
                     VERQAIETALATVYLLMTGDLAHPSAVVARDLNRWLERNKHLGQEITRRQQAARLARL
                     TADRPDERQAVEE"
     gene            complement(4992..5240)
                     /locus_tag="VFV99_22120"
     CDS             complement(4992..5240)
                     /locus_tag="VFV99_22120"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX5062089.1"
                     /translation="MLDAETAKDLIKPHMDVACPDDADFATVDQIEGHSSIKLAKDDD
                     GLHHYIPLDWVTSVDDRVHIDRSSTQARAEWTTNPPKQ"
     gene            5369..6469
                     /locus_tag="VFV99_22125"
     CDS             5369..6469
                     /locus_tag="VFV99_22125"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAGSA dehydrogenase family protein"
                     /protein_id="HEX5062090.1"
                     /translation="MHPRIAVIGASGYSGVELTKLLASHPHVELAVASSDRWVGESLA
                     AHAGVRSQLRYLALDQAIAQAAQCDVVMLATPAEASHDLVPQLVGRTRVIDLSGAFRL
                     REAWRYETHYRFKHNHPGLLKEAVYGLPELYRDKIGGARLIANPGCYATAIQLALAPL
                     MQTVDAPAGAPARPTGEGVTTRPFERVSGELLDGRIIVDAMSGVTGAGRKAAEDYSFA
                     EIAGDVRAYRVLKHQHEPEIEQSLSDIAERTIDITFVPHLLPLKRGILATCHVHYLGN
                     HDVLRLYKFAYSEEPFVTIAQSAEHVALHDVVGTNQCRIGLTAGENGELVIIAAIDNL
                     VKGAAGQAVQNMNLMLGLDEQAGLAGLRSFHP"
     gene            6466..8547
                     /gene="argJ"
                     /locus_tag="VFV99_22130"
     CDS             6466..8547
                     /gene="argJ"
                     /locus_tag="VFV99_22130"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5062091.1"
                     /translation="MKVPIGFRFAGAQAGLKPVRRDVALVVSDVPAVAAGVFTKNLAA
                     AAPIIDARPRVPSSAMRAVLINSGNANALTGPQGVTDVVAIRTALATALGIGADEVLT
                     ASTGVIGVRLPAHKLIAAVPSLVSSLTLAPEPAAEAILTTDTRTKLSSRSLTVGGKEV
                     TISAIAKGSGMIAPQLATMITVITTDVSITPALLDRAVRAAVEPSFHCLVVDGDMSTN
                     DTVIALANGQAHNPPIHDPGPDLDAFTAALTSLCTELARDIASDGEGATRLLEVAVSG
                     APSRDIARDLACSIAASNLVKAAIFGADPNWGRVLATVGARAGSQNFAVDPHKAAVAI
                     QGVPVFAKGEPVLADAPALRAKMRAPEVRVDVALAAGTGAATAWGCDLGYDYVKLNAD
                     YTSLIVQTPDGGLAKDDRLTNYSPSFKRKLIVEALSYISKFSGTRCVVKFGGSAIGKP
                     ALEQAFCDDIKLLRSVGLQPIVVHGGGHEITRTLEKLGHPAPEFIEGVRITSPSDLKV
                     VDMVLGSVNSHLVTLLNLDGGHALGISGKDGALLRAKKHAPVQGRDPGFVGEITSVNA
                     AVIEMLLGQGYVPVISPIALGDDGQSYNITADAAAAKIAIALKAQKLIYLTDAPGILE
                     HGELVTDLHVADLERKLPEQDGKSKVRMLAMIEALNNGVGRVHAIDGRTPHSLVAELF
                     TDRGVGTLITP"
     gene            8544..9014
                     /locus_tag="VFV99_22135"
     CDS             8544..9014
                     /locus_tag="VFV99_22135"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062092.1"
                     /translation="MKDRAARNRNDVTERRAVIRELIASRPVATQEELRELLAGRGHD
                     VTQATLSRDLAQLGARRAAQPGGGTSYELGDSIRTPTLVSDLVRAIDDNGTLVVVHTT
                     PGAAQVVAGVLDRAKVPEVLGTIAGDDAIFVAPARGVTTSRVIRKLRELVASAR"
     gene            complement(9023..9463)
                     /locus_tag="VFV99_22140"
     CDS             complement(9023..9463)
                     /locus_tag="VFV99_22140"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5062093.1"
                     /translation="MATINDARLREILTGSPTVAVLGIHREPEKAAFYVPEYLHDEGY
                     RVIGVNPEFVNETIFGERVLSSLRDIAEPVDLVDVFRKPEAIPNHVAEILAMPHRPKY
                     VWFQLGIKHEEAAKQLEDAGITVIQNRCTLADHQRLGLGKPERK"
     gene            9715..10554
                     /locus_tag="VFV99_22145"
     CDS             9715..10554
                     /locus_tag="VFV99_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062094.1"
                     /translation="MTSEAVAVAVAPGAGTTIVIVTRAHVAHCAALSRIADDTFVATI
                     GAGVLADNPAASPLGSSRWARARSYLINDPIAIAIERDDQRVLAVAQPKPLAGWLSID
                     AVDLPPIERYVRAWIDRQRGGALATFARNLVIESRKSQLLVRTTKLQPEELALATTDV
                     LRMLESVPTPPTVAFTCPAAGNGIVRCSGSEITVTSLASTLRKLVAVDTEPVVAAGDV
                     VGIRLTEDAEVLLRRDDVILGLDGQRIISAAQLQELARLVHDRTALAIRRDGADLIIG
                     LSE"
     gene            10600..11919
                     /locus_tag="VFV99_22150"
     CDS             10600..11919
                     /locus_tag="VFV99_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062095.1"
                     /translation="MRAFLFAALVVAACGSDKSTTPSKATPAPTPVVAPTQPAAAPKV
                     AQQEKPAAKSESSVASAEKPTQPMAGPPPGGHDFTAEGRALFIVGACGEGTSEEANAV
                     PAARRKQHCDVVNKAQSDYADKWVKKAHAFFVDKVPKDAPKTVVYPFAGGDLSTALTV
                     YPDATEITTLSLEPAGDPRTLAALKGNDLDEAMHKVEFELKWLYRVNFSNTLNMIDAM
                     RGGHLPTQLIFGLSALKVHGYELVSLRYFSIQDDGSIHYLDDADIAKAPAPTAAKSAQ
                     ERNMVFANAEVQFKKQGGGEIQVWRHIRANLGNETSKGIGKGLKDDPRVLKHLEAKGK
                     IAGMTKAASYLLSWDSFSLMRNYLVDHVVWMVSDATGVAPKWGKKAGFEYETYGTFEA
                     PHIDAGNGISTDWKQEFAAEPKRSLAFRFGYYDKKFRNHLVIMKKKA"
     gene            12022..12960
                     /locus_tag="VFV99_22155"
     CDS             12022..12960
                     /locus_tag="VFV99_22155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5062096.1"
                     /translation="MVAAACPRCGGIVPLHEGRPFVSASGAIELWHPSCWAVRHARGV
                     EEVTVIAPQPARTFPFASVAAVVGVAAVAVASYALRLKLPPASLATININSGETVALG
                     AKMSEQEIAPPVLDPRLQYPVPELDGIAMDEMYPSLLDWIHPVVDTDSALPEQMGGRF
                     GAERHGVERAECGNGHCGIDLLGPIGRPIVAVADGVVVRVEHSELGRDGRSGRYVRIQ
                     HADGSLTAYMHLNTIVDGLGVGDHVDGGQQIGTLGATAIFTAAPHCHFSLELPNVYGE
                     INGDSTSTHYVDPAPFLVRANIAHVADRRHAQKPAL"
     gene            13261..13986
                     /locus_tag="VFV99_22160"
     CDS             13261..13986
                     /locus_tag="VFV99_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062097.1"
                     /translation="MTTREDWMRRLGPLYGGAVALVHSTPWRWPLAIGNEARSSFWVV
                     ALGAPIGLVAWIAAALIHAAGMPVTVAAIVGLGVLSLASAALVERGLVERLDGTHATA
                     PSVTSILVLVFTTAVRIAAVLAVPSRAWLGVFIATALVGRWAAVFLQALGDPILDDDA
                     PRSLVATPAPAWLTAALSVGVAIVTVVALGKAGVVALALTAAIAFALGLDAQRRDRGL
                     SSPVVATAAAIGELVVLMVATLS"
     gene            complement(13979..14269)
                     /locus_tag="VFV99_22165"
     CDS             complement(13979..14269)
                     /locus_tag="VFV99_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5062098.1"
                     /translation="MSDDDEFATPALTDELDLHTFLPKECADVVEEYLHAAQAQGMRM
                     VRIIHGKGTGTLRRIVHSVLDKHPAVARYALADGASGSWGATLVELKDRPAS"
     gene            14313..16112
                     /locus_tag="VFV99_22170"
     CDS             14313..16112
                     /locus_tag="VFV99_22170"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5062099.1"
                     /translation="MGTLLAACGAPKQQAAEPTPPADLVLMNGDIYTGDPVQPRVQAL
                     AIRSGHIVAIGSNDEIRHINTQRTIDLGGRLVIPGINDAHVHEPALEEHLGAALGARV
                     GAKVTDAALDKSAGSASEPAGQATAQGAMVDLPGEFDTLTVPDMLASIERATHEQPAG
                     TWLHAVLSRAALDDPTLTREGLDRVAPAHPVWIDNFAGHALVFNSAAERAAGIDASAP
                     VPRGAFVGRDGKGGHDGWRYEYARYAALRAFGAKVSDADVNAAIRDFEARAGEFGITT
                     VQALPLQVDDARLETALRASQRTLRWHVMRVPVGEIIAPPYMLALVDQGLAGSPELEG
                     MVTVYGTKYWLDGTPIERGAALREPYADAPTDGRPPAGRADFDPAAIREMLVRARASG
                     DPLHLHIAGDVQLQALLAAMEELRPKKPGSVGDWPEQRVVIEHGDGIAAADIARIRAL
                     GIVVVQNPSHMTTPELMRARLGDRVARWMPMKSLVTSGVPFALGSDGPLNPFLNIKLA
                     TEHPANPDEALTREQALVAYTQGAAYDERTEQFKGKLITGYAADLAVLSQDIFTVPAD
                     ALPHTRSVMTIVGGTIVLDRLSPASAPTSERTE"
     gene            complement(16072..16923)
                     /locus_tag="VFV99_22175"
     CDS             complement(16072..16923)
                     /locus_tag="VFV99_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5062100.1"
                     /translation="MLTTVSAGPYTIRGVSVGGVYTSLAIPELSIVLDAGISPRSAGG
                     IDTILLSHGHADHVGALPALLGIRALHGKTKPPRVVMPAEIVADLKAALDALSKLQRW
                     PLDVDAVGMMPGDEIELRGDLLVRAYRTFHPVPSLAYMIVRRVSKLRPEFRGLPGPEI
                     AARRLAGEPMTEHEDRLELAYATDTLVSVLDHSPELLRARVLIMECTFLDERKTLEAA
                     RAGCHIHLDEVIERADKFENEHIVFMHCSQLYQPQEVAEILDKRVPPALRRRIIPFVP
                     KSEHWPG"
     gene            16984..18378
                     /locus_tag="VFV99_22180"
     CDS             16984..18378
                     /locus_tag="VFV99_22180"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5062101.1"
                     /translation="MFWYSLLVVLAVLFVLSVALPVPRRIWAFSLITAKYIWLWFLDI
                     IGLRRLWFAITGKSAKYQKLTRPVLLRMFCEDLGPTFIKFGQIIASSAGMFPDPYVKE
                     FQKVLDRVKPFPFEDVKRTLEEELGAEKAAHLVDIQPKQLASASIAQVHTAKLRDGTL
                     VVIKVQRPGIIARCMADMRIMRRVAGWVQRFKKNADLANPVGVVDDFTKTLTEELDFR
                     SEAANLDKFNEIMRELGHKHIRAPVPHHEYTTRKVLVMERFSGLRVDKFEEIKAKGID
                     GEGKLVDGLRAWFQCVVFYGFFHGDVHAGNLMLLDDNTIGFLDFGIVGRFDDKARYLV
                     TDYMIAFASGNYKRLAEIIIEMAGAPDGLDMESFVADLGTTYRPLLTLSFGEVNYADF
                     LPGIQRTATRHKMRMPNEFILITKQLLYFDRYAKSLAPKLNVFTDPRLVLGMMSDIQK
                     ARMAHEAKTKNATA"
     gene            complement(18375..18761)
                     /locus_tag="VFV99_22185"
     CDS             complement(18375..18761)
                     /locus_tag="VFV99_22185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5062102.1"
                     /translation="MGSIDRQHPRYAADVGIKFYVGKQVMDGRTKNVSRGGLCADLAN
                     ALPIGSHVEIELTLKFDDASQSEPLRLPVRIVWCTPVEDAFQVGLSFRGLDKQRAEYL
                     TLFLKYLGDEKPMRSKKPSNIDDRFG"
     gene            complement(18873..19586)
                     /locus_tag="VFV99_22190"
     CDS             complement(18873..19586)
                     /locus_tag="VFV99_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062103.1"
                     /translation="MRTVRSVARARNQPRGHLALIVVAVVALLAVLVARANAAPRDDE
                     DDAPPGASLELPGEYGGDPDRPSPQRHLYVPITNAMIERIATFAPPIASVVAAAYRAG
                     GVADDPTPSWRRRSRLAALVPMVSVRAGQNQAWRDVDDPTISHGVGIDVRASWRLDQL
                     LFDRDEPRIAMLDVARRRERRRLAAQAIHLYYDWVAARVAADADIRAELDAQEKAAEL
                     DALTAGWFSQALAKRAELR"
     gene            19587..>21040
                     /locus_tag="VFV99_22195"
     CDS             19587..>21040
                     /locus_tag="VFV99_22195"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX5062104.1"
                     /translation="MSTTLTENALQVLRARYLARENGVVVETPEQLFRRVARHVSAVE
                     GALGYSPAEVDSARAKFERMMVALEFVPNSPTLMNAGRPLGQLAACFVVPVDDSTTGV
                     FEAVRWAAEIQKTGGGTGFSFSRLRPAGDLVASTGGNASGPVSLMQVFDVATEAIKQG
                     GTRRGANMGMLRVDHPDILEFIALKLDPTRMRNFNLSVAATDTFMKAVAAGTTYDLVN
                     PRTKIVTGQLDARRVFDAIANAAWACGDPGLVFIDRVNDLHPTPALGPIEATNPCVTG
                     ESLVWTYARGLVPIRDLVGTQPRVATLDGDEVVFRTATQVVCTGYRKVYRLRTGSGLS
                     LRLTADHRVATDCGDVAAEHLEVGMRVRAIENHRVGHGHGRAIATAGNMGRRPLRADA
                     LDVPRERLGELLGWTTVVALEPDGCELVYDLTEPTTSHFLANGMLVHNCGEQPLLPFE
                     SCTLGSIDVGRFVAGRDFVWERLREAIHDSVRFL"
BASE COUNT         3419 a         7105 c         7111 g         3405 t
ORIGIN      
        1 cttgcgaggc gacgtcgccg acgagtggcc aaaattcgag ggcagggcgg agggcgagcg
       61 ttgcgtcgcc aagccggcac gtgatgccgg gcgcgcgcca tgcttcgagc tcgcggcgca
      121 gctcggcggg aatgccgagg ccgtgctcgt cgagatcgcc gagcacgatg cgcagatcgc
      181 ggtggagtgc gagcggtagc gcgaagcgat cgtgaagctc gtcgtgccag tcgacgagcg
      241 gcgcgcggta gtgattgacg acgaggcgcg cgacgatcga ccggaacaac acggcgagtg
      301 cggcgagcat gccggggcgc tcgggcatgc gaatcgcgcg cagctcgacg acgcccatgc
      361 ggccgtgacg cggaccgtgc accgagatgt gagggttcca cagcttctcg acgttgagct
      421 cggcgcgatg cgagttgccc gcggaatcaa cgagcaatgg gccgagccct tgctggagct
      481 gctcgggcgg aagctcgccg cgatccgcga ggtgctcgag ccagccgagt gcgaccgcga
      541 gctcgtccga gcgctcacgc gtgccctcgt cgggacgtgg accctgcgac gcggcgccga
      601 cgcactcgct cgcgaaccag taggagaggg acgggtggtt gttgaggtag cggaccagcg
      661 ccggcagcac gtgcgggtag cgcatgaacg gcgagctgtc gggtcgcgag ccgccgagcg
      721 tgagctggcc accaccgccg gagtcggcga ggtcgccgtt gaagcgaaag cgcgtcgctg
      781 acagaccggc ggcaccggcg gcggtccaga tccgttgcgc ttgcgcgtag agctcgcgcg
      841 tcgtcgcgca cggcgccatg ttgacctcga cgacgccggg atccggcgtg accgagagcc
      901 agcgatcggc gccgagatcg gtcgggggtg gtacggcgac cgcatcggcg gtcgtgcgct
      961 cgccgttggt cgctgcgatg ttgtcctcgc gccagcggac gccgaacgcg aagcgcggct
     1021 gcgtctcgtc gggaaactgg cggccgatca cgcgtgcgac cgacgcgcct ggcgtgcgat
     1081 cggcgaacgc ggtcgcgagt gcgtgcgcgc gcgcgagctt gtcgctgccc tcggcgttgg
     1141 agctccacgc gggatcgagc gactcgggac gcgtgaacgt cggctcggca ccgatccaga
     1201 tctcgacgcc gcggcgcgcg atcagctcgt cgtgcatcgc gagcgaggcg tcgaggtcgt
     1261 cgctcactcg gtgatccatt cctccgcgcg tggcatcggc ttgccggcat cgagccggcg
     1321 gaggtcgagc gtgtacggct gctcgggatg cggttccgcg gtgcgcgcac gaaccggcca
     1381 cggcgcgggg ccttcgagcg tccagcgctg agcgcggcgc gccgaggctt cgacgcgtgt
     1441 aagcggcggc gcatcgtacg cgcggccttc gggatgccag acgtgatacg tgccaccggc
     1501 gacaccgcgc ttcgcccacg tatcgatgat gtcgacgcgc accgggtgat gaatgccgag
     1561 gtgaggatgc agcgcatgcg gcgggcacca cgcacggaag cgaacaccgc cgacgcgaac
     1621 atcgcggccg ccggacgcgc ggcgcatcgg cagagtgacg ccgttgatgg tgatgacgta
     1681 gcgttcctcg tcgaggccgt gcgaacggat ctcgagccgt tccatcgacg aatcgacgta
     1741 gcgtgccgtg ccggtctgcg tcgcttcttc gccgagcacg tgccagggct cgatcgcgtt
     1801 gcgcacttcg acgcgagcgc cgccggtctc gagcgcgccg acaagcgggc accgcaggtc
     1861 gacgaacgga cggtaggcat cggcaggcag tgcgacgtcg tgcgctgcga gatgcgcgag
     1921 cacgtcctcg aagtcgcgcc acatgtagta cggcaacagg aagcggtcgt gcagctccgc
     1981 gccccagcgc acgagcgcgt gcttgtacgg ctgtttgacg aacgacgcga gcaacccgcg
     2041 cgtcagcacg acctgcgccg cggccatccg gggatgcggc ggcatctcga acgcgcggag
     2101 ctcgacgagc ccttgccggc catgcggcgt cagcggatcg aacagcttgt cgatcgagat
     2161 ttcggcgcga tgcgtggagc ccgcgacgtc gacaagcagg tggcggagga ccgcgtcgat
     2221 ctgccaggtc ggcggggcat tctgcagatc gaagaggtgc ggcagcgcga cctcgagctc
     2281 gtagagtgcg tcgtggcgcg cttcgtcgac gcgcggcgct tgcgacgtcg ggccgacaaa
     2341 caggccggtg aacatgtacg acaacgacgg gtggtgctgc gcgaacgtga tcaagctcgc
     2401 gagcgtgtcg ggacggacga gccacgggga tgcgagcggc gtcggaccac cgatcgtgat
     2461 gtggttgccg ccgcccgagc cggccatgcg gccatcgagc atgtagcgtt cggcagtcag
     2521 gccgctcgcg agcgcggcgt cgaacacggt cgcgtggaca tccgccgctt cgcgcgcgct
     2581 gtacgtcggc ggcagattga cctcgagcac accaggatcc ggcgtgacgc cgaagcggcg
     2641 caagttcggc gacggcggcg ggccgtagcc ttcgaggttg accgtgaaac cggtcgccgc
     2701 gcgcgcgcga tcgatcgcgt tgatgagcgc gcagaaatct tcgaagcggc cgaccggcgg
     2761 caggaacaac cacagctcgc cgtcgcgcgg ctcgatcgcg agcgcggtgc gaatgccgat
     2821 gcccggttcg ccgggccgcg cgatgcgttt tggcgtactc cggcgttcgg ccgcttgcgc
     2881 cttctcgtct gcgtcgtcgg gattgcgacg cggatcgtcg acgtcgggtt cctcccacag
     2941 cggcggcgcc ggcgatgcgg cgaggctgcc gagcggcagg cgcagaccca ccggactatc
     3001 gcccggtaga aggaacagct cgccgcggcg gaagctccac cgctcggtga gccagctacc
     3061 ctgcgcgctg cgcgcgagcg gaagcaccca gccgacgacg ctgccgacac cttgaccgaa
     3121 gatcttcgcg agtcgccgac gttcttcggg atcgtcgagg ccggccttgc gtggatcgat
     3181 atcgggcggc agctgcgctt cgccgcgcac gacctcccac ggatcttcga acgcgccgtg
     3241 cagctcagta ctcacgccga gtgcagccgc cagctgctcg ccgaagcgtc gcgcatcgtc
     3301 cgcaccgatg cagtcgcgca gctcgcgctg cggccacatc gcgacgccat cgcgccgcgc
     3361 gatcacgtcg agcgcccagc gcggcatgct ctcgccggga tactgcttgc cctggcgatg
     3421 cagcacgagc ccgcccggtg cgaggcgatc gagtagctcg tccgcgagcg cacgcccgcg
     3481 cttccacttg tcgggaccga gtgcgccgcc ttgccactcg gcagccgcga cgttttcgcg
     3541 tgcggtgaac gtcggctcgc cgccgaccgt cacgttcagg cccgccgcgc cgagcgccgc
     3601 atccgcgcga tcgccggccg cgagcaagtc gctccatacc tcgtcggtga acggtgcagt
     3661 gggccgcgtc tcgtgcccga gccgcgcgat tgccgtcgag aactcgaccg cgctcgcacc
     3721 gacgttgctc gtgccgtcga gcggcgacgc gtgcgccggc gatgccgtgc acgcgagcgg
     3781 gatgtgacct tcgccggtaa gcaggccgct ggtgccgtcg agtccgatcc agcccgcgcc
     3841 gggcagatac gcctcggccc acgcgtgcag atcgacgacg tcgcggctga cgcccttcgg
     3901 ctcgttgggg atcatgccct cgtcggtgag ctggatcaga tagcccgatg cgaatcgcgc
     3961 cgcgatgccg cgcgcccgga gcagcgcgac gagcagtacc gcgctgtcgc gacagctacc
     4021 gcgcccgttc tgcagcgtct cctccggcgt ccacacgccg gcttcatcgc gaatcacgta
     4081 cgcgacgcgc tccttgacca gccgattgac ctcgacgagc aggccgaccg tgtcgccggt
     4141 cgccgggact ttctcgagca gctcgaccgc gagccggccg agccggtacg ccggatcggt
     4201 gacgtcgagg aacacgccga gctcggcggc gagatcatcg ggatactgga atgggatcgt
     4261 cttcgcgcga tcgtcgacga agaaatcgaa cgggttgacc ggatgaacgt cgaccgcgag
     4321 ctcgacgacg atgtcgagct ccgtcacggg ctggccggcc ttgaacgtca cgcgcgcgac
     4381 gcgattgccg tgcggatcgc gctgccagtg caggcgatgc tccggcgcca cgtccaggcg
     4441 gtagctctcg atgcgcgctc gcgcgtgatc ggcgggacgg aggcggatca attgtggccc
     4501 gagcagcgcg ggccggggat agacataacg actgcgatgc tggaggagca cacgcacggc
     4561 tgaagggtat taccaccgcg cgcccaggta cggttccgga ggggcgcttg actgagcgtc
     4621 atcgctcagc gcgcgctact cctcgacggc ttggcgctcg tcgggccggt cggccgtcag
     4681 ccgcgcaagc cgtgcagcct gctgccggcg ggtgatttcc tgaccgaggt gcttgttgcg
     4741 ctcgagccac cggttcaagt cgcgcgcgac gaccgccgac ggatgtgcga ggtcgccagt
     4801 catcagcaaa tagacggtcg ccaacgccgt ttcgatcgcc tgccgttcga cgatgtcgta
     4861 cggaccgagc tccgcgagcg catcctcgag ctcgtccttg cgtgcgagca cgcgcatcgc
     4921 gagtggctga ccacgcgtcg cgtcgacgga gttttcgagc ttttggctga tcacgccgct
     4981 catcgcgcct cctactgctt cggcggattc gtggtccatt cggcccttgc ctgcgtgctg
     5041 ctccggtcga tgtgaactct gtcgtctacc gacgtgaccc agtcgagcgg gatgtagtgg
     5101 tgaagcccgt catcgtcctt ggccagcttg atcgagctgt gaccctcgat ctggtcgacg
     5161 gtcgcaaaat ccgcatcatc cgggcacgcg acatccatgt gcggcttgat gagatctttc
     5221 gctgtctcgg cgtccagcat gtccccagat ggagcatcgt gcgtgccaga cggacgcgtc
     5281 tccgtcgggc gcggtagcct cagcgaggca ggtccgcaca gatcgggctt gcatgcgtgc
     5341 atataatgtg catattcatg caacgaatat gcatccacgc attgccgtca tcggcgcatc
     5401 cgggtactcg ggtgtcgagc tgaccaagct gctcgcctca cacccgcacg tcgagctcgc
     5461 ggtcgcgtcg agtgatcgct gggtcggcga gtcgctcgcc gcgcacgcgg gtgtacgcag
     5521 ccagctgcgc tacctcgcgc tcgaccaggc gatcgcgcaa gccgcccagt gcgatgtggt
     5581 gatgctcgcg acgcccgccg aggcatcgca cgacctcgtg ccgcagctcg tcgggcgcac
     5641 gcgcgtcatc gatctgtccg gcgcgtttcg cctgcgggaa gcatggcgct acgagacgca
     5701 ctaccgcttc aagcacaacc atcctggcct gttgaaggaa gccgtctacg gcttgcccga
     5761 gctgtatcgc gacaagatcg gtggcgcgcg gctgatcgcg aatcccggtt gctatgcaac
     5821 cgcgatccag ctcgcgctcg cgccgctgat gcaaaccgtc gacgcaccgg ccggcgcccc
     5881 tgcgcggccg accggcgaag gcgtgacgac gcgcccgttc gagcgcgtct cgggtgagct
     5941 gctcgacggc cgcatcatcg tcgacgcgat gtccggcgtg accggtgccg gccgcaaggc
     6001 ggcggaggac tacagcttcg ccgagatcgc cggcgacgtt cgcgcgtatc gggtgctcaa
     6061 gcaccagcac gagccggaga tcgagcagag cctcagcgac atcgccgagc gcacgatcga
     6121 catcacgttc gtgccgcatc tgttgccgct caagcgcggc atcctggcga cgtgccacgt
     6181 gcactacctc ggcaaccacg acgtgctgcg cctctacaag ttcgcgtaca gcgaagagcc
     6241 gttcgtcacg atcgcgcaga gcgccgagca cgtcgcgttg cacgatgtcg tcggcacgaa
     6301 ccagtgccgc atcggcctca ccgccggcga gaacggcgag ctcgttatca tcgcggcgat
     6361 cgacaacctc gtgaagggcg cggccggtca ggccgtgcag aacatgaact tgatgctcgg
     6421 tctcgacgag caagcaggcc tcgcgggtct gcggagcttc catccatgaa ggtacctatc
     6481 gggtttcgct tcgccggtgc gcaggctggt ctcaagccgg tgcggcgtga tgtggcgctc
     6541 gtggtgtccg acgtgccggc cgtggccgcc ggcgtgttca cgaagaacct cgctgccgcg
     6601 gcgccgatca tcgatgcgcg tccgcgcgtg ccgtcgtctg cgatgcgcgc ggtgctgatc
     6661 aacagcggca acgcgaatgc gctgacggga ccgcagggcg tgacggacgt cgtcgcgatc
     6721 cgcacggcgc tcgccacggc gctcggcatt ggcgccgacg aggtactcac cgcctcgacg
     6781 ggcgtgatcg gcgtgcggct tcccgcgcac aagctgatcg cggcggtgcc gtcgctcgtc
     6841 agctcgctga cgctggcacc cgagccggcc gccgaggcga tcctgaccac cgatacgcgc
     6901 acgaagctgt cgtcgcggtc gctcaccgtc ggtggcaagg aggtcacgat ctcggcgatc
     6961 gcgaagggct cgggcatgat cgcgccgcag ctcgcgacga tgatcacggt gatcacgacc
     7021 gacgtctcga tcacgccggc gctgctcgac cgcgcagtgc gcgcagcggt cgagccgagc
     7081 ttccactgct tggttgtcga cggcgacatg agcacgaacg acaccgtgat cgcgctcgcc
     7141 aacggccagg cgcacaaccc gccgattcac gatccgggtc ccgatctcga cgcattcacc
     7201 gcggcgctga catccctgtg caccgagctg gcgcgtgaca tcgcgtccga cggcgagggt
     7261 gcgacgcgtc tgctcgaggt ggccgtcagc ggcgcgccat cgcgcgacat cgcgcgtgac
     7321 ctcgcatgct cgatcgcagc atcgaatctg gtgaaggcgg cgatcttcgg cgccgatccg
     7381 aactggggcc gcgtgctcgc gaccgtcggg gcgcgcgccg gctcgcaaaa cttcgccgtc
     7441 gatccgcaca aggctgcggt cgcgatccaa ggcgtgcccg tgttcgcgaa gggcgagccc
     7501 gttctcgccg atgctccggc cctgcgcgcg aagatgcgcg cgcccgaggt acgcgtcgac
     7561 gtcgcgctgg ctgcaggcac cggcgctgcc accgcatggg gctgcgacct cggctacgac
     7621 tacgtcaagc tcaatgcgga ctacacgtcg ctgatcgtgc agacgccgga cggtgggctc
     7681 gcgaaggacg atcggctcac gaactacagc ccgagcttca agcgcaagct gatcgtcgag
     7741 gcgctcagct acatctccaa gttctcgggc acgcgctgcg tggtcaagtt cggcggcagc
     7801 gcgatcggca agccggcgct cgagcaagcg ttctgcgacg acatcaagct gctgcgctcg
     7861 gtcggtctgc aaccgatcgt cgtgcacggc ggtggccacg agatcacgcg cacgctcgag
     7921 aagctcggcc atcccgcgcc cgagttcatc gagggcgtgc gcatcacgtc gccgagcgac
     7981 ctcaaggtcg tcgatatggt gctcggctcc gtgaattccc acctcgtcac gttgctcaac
     8041 ctcgacggtg gccacgcgct cggcatctcc ggcaaggacg gcgcgctact ccgcgcgaag
     8101 aagcacgcgc ccgtacaggg ccgcgatcct ggtttcgtcg gcgagatcac gagcgtcaac
     8161 gccgcggtga tcgagatgct gctcgggcag ggttacgtgc cggtgatctc gccgatcgcg
     8221 ctcggcgatg acggccagag ctacaacatc accgccgatg ccgcggcggc gaagatcgcg
     8281 atcgcgctga aggcgcagaa gctcatctat ctcaccgacg cgccgggcat cctcgagcac
     8341 ggcgagcttg tcaccgacct ccacgtcgcc gacctcgagc gcaagctccc cgagcaagac
     8401 ggcaagtcga aggtgcgcat gctcgcgatg atcgaggcgc tgaacaacgg cgtcggtcgc
     8461 gtgcacgcga tcgatggccg tactccgcac agcctcgtcg ccgagctgtt caccgaccgt
     8521 ggtgtcggta cgctgatcac gccatgaagg accgcgctgc gcgcaaccgc aatgacgtga
     8581 ccgagcgccg cgcggtcatt cgcgagctga tcgcgagtcg gcccgtcgcg acgcaggaag
     8641 agctgcgcga gctgctcgcc ggccgcggtc acgacgtcac gcaagccacg ctgtcgcgcg
     8701 acctcgccca gctcggagcg cgacgcgctg cccagcccgg cggcggcacg tcctacgagc
     8761 tcggtgacag cattcgcacg ccgacgctcg tcagcgatct ggtccgcgcg atcgacgata
     8821 acggtacgct ggtcgtcgtt cacacgacgc ccggcgcggc gcaggtggtt gctggcgtgc
     8881 tcgatcgggc gaaggtgccc gaggtgctcg gcacgatcgc aggcgacgat gcgatcttcg
     8941 tggcgcccgc tcgcggcgtc accacgtcgc gcgtgattcg caagctacgc gagctggtcg
     9001 cgagcgcgcg ttaatagcca gctcacttgc gctcgggctt gccgaggccg agacgctggt
     9061 ggtcggcgag tgtgcaacgg ttctggatca ccgtgatgcc ggcatcttcg agctgcttcg
     9121 cagcctcctc gtgcttgatg ccgagctgga accagacgta cttcggtcga tgtggcatcg
     9181 ccaggatctc ggcgacgtga tttggaatgg cttctggttt gcggaacacg tcgactaggt
     9241 cgaccggctc tgcgatgtcg cgcagggaag acaacaccct ctcgccgaag atggtctcgt
     9301 tgacgaactc cgggttcacg ccgatgactc gatagccctc gtcgtgcaag tactccggca
     9361 cgtagaaggc cgccttctcc ggctcgcgat ggatcccgag cactgcaacc gtcggcgacc
     9421 ccgtcaagat ctcccgcaag cgggcgtcat tgatggtggc catgggccgc accttaccaa
     9481 gcatctgcta cagagaaagg ggtgaggatg tggccactgg tggtgatctc aacggcgctc
     9541 gctgcgtgcg cctccaccac acctactgag cgcgagcgcg cactcggcag acttccatcg
     9601 caagcacagc tggttgcagc ggccgatggg acggccctcg ctgccttgcg gcccgcaatt
     9661 gatgcggctc ggccgctttt gccaaccaag ctcgactgcg tagtcgaggc tgcgttgact
     9721 agcgaagcgg ttgcggtcgc ggtcgcgcca ggcgcgggaa cgacgatcgt catcgtcacg
     9781 cgtgctcacg tcgcgcattg cgcagcgctc agccggatcg cggacgatac gttcgtcgcg
     9841 accatcggcg ctggtgtgct cgccgataat ccagctgcat caccgctcgg cagctcgcga
     9901 tgggcacgag cgcggtcgta tctgatcaat gatccgatcg cgatcgcgat cgaacgcgat
     9961 gaccagcgcg tgctcgcggt cgcgcaaccg aagccactcg cgggttggct ctcgatcgat
    10021 gcggtcgacc tgccaccgat cgagcgctac gtgcgcgcgt ggatcgatcg ccagcgcggc
    10081 ggcgcgctcg caacgttcgc gcgcaacctc gtcatcgagt cgcgcaagag tcagttgctc
    10141 gtgcgcacga ccaagctgca accggaagag ctcgcgctcg cgacaaccga tgttctgcgc
    10201 atgctcgagt cggtgccgac accaccaacg gttgcattca cgtgtcctgc ggcgggcaac
    10261 gggatcgtgc ggtgcagcgg cagcgagatc accgtgacgt cgcttgcctc gacgttgcgc
    10321 aagctcgtcg cggtcgacac cgagccggtc gttgccgccg gcgacgtggt cgggattcgt
    10381 ttgacagaag atgccgaagt gctgcttcgt cgcgacgacg tcatcctcgg cctcgacggc
    10441 cagcgcatca tcagcgccgc gcaactgcag gagctcgctc gcctcgtcca cgaccggacc
    10501 gctcttgcaa ttcgccggga cggcgccgac ctcatcatcg gcctctccga ataatcggcg
    10561 atagcaaacg cttagggcgt gttgcatagt cgggcgcgca tgcgtgcctt cctgttcgcc
    10621 gcactcgtcg tcgcagcctg cggtagcgac aagtccacga ccccgagcaa agcgacgcca
    10681 gcaccgacac cggtcgtcgc gccgacgcag ccggccgccg cgccgaaggt cgcacagcag
    10741 gagaagccgg ccgcaaagtc cgagtcgtcc gtcgcttcgg cagagaagcc gacgcagccg
    10801 atggccggtc caccgcctgg tggtcacgac ttcacggccg agggcagggc gctgttcatc
    10861 gtcggcgcgt gcggtgaagg cacgagcgaa gaagcaaacg cggtcccggc ggcgcggcgc
    10921 aagcagcatt gcgacgtcgt caacaaggcg cagtccgact acgccgacaa atgggtgaag
    10981 aaggcgcacg cattcttcgt cgacaaggtg ccgaaggacg caccgaagac agtcgtctat
    11041 ccgttcgcgg gtggcgatct gtcgaccgcg ctcacggtct atcccgacgc caccgagatc
    11101 acgacgctgt cgctcgagcc cgcgggtgat ccgcgtacgc tcgccgctct caagggcaac
    11161 gacctcgacg aggcgatgca caaggtcgag ttcgagctca agtggctcta ccgcgtgaac
    11221 ttcagcaaca cgctcaacat gatcgacgcg atgcgcggcg gtcatttgcc gacgcagctg
    11281 atcttcggct tgtccgcgct caaggtgcac ggctacgagc tcgtgtcgct ccgctacttc
    11341 agcatccagg acgacggctc gattcactac ctcgacgacg ccgacatcgc gaaggcgccg
    11401 gcgccgaccg ctgcgaagag cgcccaggaa cgcaacatgg tgttcgcgaa tgccgaggtg
    11461 cagttcaaga agcaaggcgg cggcgagatc caggtctggc gacacattcg cgcgaacctc
    11521 ggcaacgaga ccagcaaggg catcggcaag ggcctcaagg acgacccgcg cgtgctcaag
    11581 catctcgagg cgaaagggaa gatcgccggc atgacgaagg cggcgagcta cctgttgtcg
    11641 tgggactcgt tctcgctgat gcggaactac ctggtcgacc acgtcgtgtg gatggtctcg
    11701 gatgccactg gggtagcgcc caaatggggc aagaaggccg gtttcgagta cgagacgtac
    11761 gggacgttcg aggcgccgca cattgatgcg ggcaacggca tctcgacgga ctggaagcag
    11821 gaattcgccg cagagccaaa gcgatcgctc gcgttccgct tcggctacta cgacaagaag
    11881 ttccggaacc acctcgtcat catgaaaaag aaggcgtgag ctgcgctgct gatccggcgc
    11941 tggtaggaca cgtctccaca cgaaaaaaat gatcccgcgg tggcgatcgc ggcgatcgcg
    12001 actcggctag catcgtgtgg aatggtggcc gctgcctgtc cgcgttgtgg agggattgtc
    12061 ccgcttcacg aaggtcggcc attcgtttcc gcgagcggcg cgatcgagct gtggcatccg
    12121 agctgctggg cagtgcggca tgcgcgcggc gtcgaagagg taaccgtgat cgcgccgcag
    12181 ccagcgcgga cgtttccgtt cgcgagcgtt gcagcggtcg tcggcgttgc agcggtcgcc
    12241 gtcgcgtcgt acgcgctgcg cttgaagcta ccgccggcat cgctcgcgac gatcaacatc
    12301 aacagcggcg agacggtcgc gctcggcgcg aagatgagcg agcaggagat cgcgccgccg
    12361 gtgctcgatc cgcgcctgca gtatccggtg cccgagctcg atggcatcgc gatggacgag
    12421 atgtatccgt cgctgctcga ctggattcac ccggtcgtcg acaccgatag cgcgttgcca
    12481 gagcaaatgg gtggccggtt cggcgccgag cgccacggcg tcgagcgcgc ggagtgtggc
    12541 aacggccatt gcggcatcga tctgctcggc ccgatcggcc gcccgatcgt tgccgtcgcc
    12601 gacggcgtcg tcgtccgtgt cgagcattcc gagcttggcc gcgatggtcg cagcggccgc
    12661 tacgtgcgca tccagcacgc cgacggctcg ctgaccgcgt acatgcacct caacacgatc
    12721 gtcgatggtc tcggggttgg cgaccacgtc gatggtggcc agcagatcgg tacgctcggc
    12781 gcgactgcaa tcttcacggc cgcaccgcat tgccacttct cgctcgagct gccgaacgtc
    12841 tacggcgaga tcaacggcga cagcacgagc acgcactacg tcgatccggc gccgtttttg
    12901 gtgcgcgcga acatcgcgca cgtcgccgat cgccgtcatg cgcagaagcc cgcgctctga
    12961 gctcgtaggt gagcgtacgc ggttccgtgc gacccggtgc gtggctcgat ctcggcgaag
    13021 gccggagctg cgtcgactcg agcggcaagg ccgctgtgaa cggtcggaac ctcgagtcgc
    13081 gggcgatgct cgaacggcgt tgacccgagc gctcggaatt gtgacggctc ggaagttcgg
    13141 gtcgacgccc agaacaggac gatgcatccg gcgagacggc tcctcggtcg gatcgccgct
    13201 tacgcttacg cttacgccta cgcctacgtt cggatctgac tctcgacgta tagtcggggc
    13261 gtgaccacgc gcgaagactg gatgcgccgg ctcggcccgc tctacggcgg tgcggttgcg
    13321 ctcgtgcatt cgacgccgtg gcgatggccg ctcgcgatcg gtaacgaggc gcggtcgtcg
    13381 ttctgggtcg tcgcgcttgg cgcgccgatc ggtctcgtcg cgtggatcgc cgcggcgctg
    13441 atccacgcgg ccggcatgcc ggtcaccgtc gctgcgatcg tcggcctcgg ggtgctgtcg
    13501 ctcgcatccg cggcactcgt cgagcgcggg cttgtcgagc gactcgacgg cacgcacgcg
    13561 accgcgccga gcgtcacgtc gatcctcgtg ctcgtgttca cgaccgcggt acgcatcgcg
    13621 gcggttctcg cggtgccgtc gcgcgcctgg ctcggtgtgt tcatcgcgac cgctctcgtc
    13681 ggccgctggg ccgccgtgtt cttgcaagcg ctcggcgatc cgatcctcga tgacgacgcg
    13741 ccgcgctcgc tcgtcgcgac gcccgcaccc gcgtggctca ccgccgcgct cagcgtcggt
    13801 gtcgcgatcg tcaccgtggt cgcgctcggc aaggctggcg tcgtcgcgct ggcgctcact
    13861 gcagcgatcg cgttcgcgct cgggctcgat gcgcagcgcc gcgaccgcgg cttgtcgtcg
    13921 ccggtcgtcg cgacggccgc tgcaatcggc gagcttgtcg tactgatggt cgcgacgctc
    13981 agctagccgg ccgatccttc agctcgacga gcgtcgcgcc ccagctgccg ctcgcgccat
    14041 cggcgagcgc atagcgcgcc acggccgggt gcttgtcgag cacgctgtga acgatgcggc
    14101 gcaacgtgcc ggttcctttg ccgtggatga tccggaccat gcgcatgccc tgtgcctgcg
    14161 ctgcgtgcag gtattcctcg acgacatccg cacactcctt cggcaggaat gtgtggagat
    14221 cgagctcgtc ggtcagcgcc ggcgtcgcga attcgtcgtc gtcactcaaa caatgcgatg
    14281 atagggcaat gtgtcggggg tggctggcga cgatggggac tctccttgcg gcgtgcggcg
    14341 cacccaagca gcaggcggcc gaacccacgc cgcccgccga tctggtgctg atgaacggcg
    14401 acatctacac cggcgatccg gtgcagccgc gcgtccaggc gctcgcgatc cgtagcggtc
    14461 acatcgtcgc gatcggtagc aacgacgaga ttcgtcacat caatacgcaa cgtacgatcg
    14521 atcttggcgg tcggctcgtg attcccggca tcaacgacgc gcacgtccac gagcccgcgc
    14581 tcgaagagca cctcggtgcg gcgcttggtg cgcgcgtcgg cgcgaaggtc accgacgccg
    14641 ctctcgacaa gagcgccggc agtgcgagcg agcccgcggg acaggcgacg gcgcagggcg
    14701 cgatggtcga cctgccgggc gaattcgaca cgctgacggt gcccgacatg ctggcgtcga
    14761 tcgagcgcgc gacccacgag caaccggcgg gcacgtggct gcacgccgtg ctcagccgcg
    14821 ccgcgctcga cgatccaacg ctcacgcgcg aagggctcga tcgcgtcgcg cccgcgcatc
    14881 cggtgtggat cgacaacttc gccggccacg cgctcgtctt caacagcgcc gcggaacgtg
    14941 ccgccggcat cgacgcgagc gcgccggttc cgcgcggcgc cttcgtcggc cgcgatggca
    15001 agggcggaca cgatggctgg cgctacgagt acgcgcgcta cgctgcgctc cgtgccttcg
    15061 gcgcgaaggt cagcgacgcc gacgtcaacg ctgcgatccg cgacttcgag gcgcgcgccg
    15121 gcgaattcgg catcaccacc gtgcaggcgc tgccgctgca ggtcgatgac gcgcggctcg
    15181 agaccgcgct ccgtgcctcg cagcgcacgc tgcgctggca cgtcatgcgc gttccggtcg
    15241 gcgagatcat cgcgccgccg tacatgctcg cgttggtcga ccaggggctc gccgggtcac
    15301 ccgagctcga gggcatggtc accgtgtacg gcacgaagta ctggctcgat ggcacgccga
    15361 tcgagcgcgg cgctgcgctg cgcgagccgt acgccgatgc gccaaccgac ggccggccgc
    15421 cggcaggtcg cgccgacttc gatccggctg cgattcgcga gatgctggtg cgcgcgcgag
    15481 cgagcggcga tccgctgcac ctgcacatcg cgggtgacgt gcagctccag gcgctactgg
    15541 ccgcgatgga agagctgcgg ccgaagaagc cgggctccgt cggtgactgg ccagagcaac
    15601 gcgtcgtgat cgagcacggc gacggcatcg cggctgccga catcgcgcgc attcgcgcgc
    15661 tcggcatcgt cgtcgtccag aacccgtcgc acatgacgac gcccgagctc atgcgtgctc
    15721 ggctcggtga tcgggtggcg cgctggatgc cgatgaagtc actcgtcacc tccggcgtgc
    15781 cgttcgcgct cggctccgac ggcccgctca atccgttcct caacatcaag ctcgcgaccg
    15841 agcatccggc gaatcccgac gaggcgctga cccgtgagca ggcactcgtc gcgtacacgc
    15901 aaggcgctgc ctacgacgag cgcaccgagc agttcaaagg caagctcatc accggctatg
    15961 ccgcggacct cgcggtgcta tcgcaggaca tcttcacggt gccggccgac gcactcccgc
    16021 atacgcgcag cgtgatgacg atcgtcggcg gcacgatcgt gctcgatcgg ctcagcccgg
    16081 ccagtgctcc gacttcggaa cgaacggaat gatccggcgc cgcagcgcgg gcggtacgcg
    16141 cttgtcgagg atctcggcca cttcctgcgg ttgatacagc tgcgagcagt gcatgaacac
    16201 gatgtgctcg ttctcgaact tgtcggcgcg ctcgatcact tcgtcgagat ggatgtggca
    16261 gccggcgcgt gcggcttcga gcgtcttgcg ttcgtcgaga aacgtgcact ccatgatcag
    16321 cacgcgcgca cgcagcagct cgggcgagtg atcgagcacc gacaccagcg tgtccgtcgc
    16381 gtacgcgagc tcgagccggt cttcgtgctc ggtcatcggc tcccccgcga gccggcgcgc
    16441 cgcaatctcg ggtcccggca gcccgcggaa ctcggggcgc agcttcgaca cccgccgcac
    16501 gatcatgtac gcgagcgacg gcaccggatg gaacgtgcga tatgcgcgaa cgagcaagtc
    16561 gccgcgcagc tcgatctcgt cgccgggcat catgcctacg gcatcgacat cgagcggcca
    16621 ccgctggagc ttggacagtg cgtccagtgc cgccttcaga tcggccacga tttcggctgg
    16681 catgacgacg cgaggcggct tcgtcttccc gtggagcgca cgaatgccga gtaatgccgg
    16741 caacgctccg acgtgatcgg cgtgcccgtg cgacagcagg atcgtgtcga ttccaccggc
    16801 cgagcgcggc gaaatccctg catcaagaac aatgctgagc tcgggaatcg ccaagctcgt
    16861 gtacacgccg ccgaccgaaa cgcctcggat cgtgtacggt cccgctgaga cagtcgtcag
    16921 cacccggcaa gtctacttgc cactggacgt gtgcgtgcac acacctgcta ggattagcgc
    16981 cgcatgtttt ggtactccct gctagtcgtt ctggcggttc tgttcgtgct gtcagtcgcg
    17041 ctgccagtgc cgagacgaat ctgggcgttc tcgctcatca cggcgaagta catctggctc
    17101 tggttcctcg acatcatcgg cctgcgccgg ctctggtttg cgatcaccgg taagagcgcc
    17161 aagtaccaaa agctcacgcg cccggttctg ctccgcatgt tctgcgagga cctcgggccg
    17221 acgttcatca agttcggtca gatcatcgca tcgagcgcgg gcatgttccc cgacccgtac
    17281 gtcaaggaat tccagaaggt cctcgaccgc gtgaagccgt tcccgttcga ggacgtcaag
    17341 cgcacgctcg aagaggagct cggcgccgag aaggccgcgc acctcgtcga catccagccc
    17401 aagcaactcg cgtccgcgtc gatcgcacag gttcacaccg cgaagctgcg cgacggcacg
    17461 ctcgtcgtca tcaaggtcca gcgccccggc atcatcgcgc gctgcatggc ggacatgcgc
    17521 atcatgcgtc gcgtcgccgg ctgggtgcag cgcttcaaga agaacgccga cctcgcgaac
    17581 cccgtcggtg tcgtcgacga cttcacgaag acgctcaccg aagagctcga cttccgcagc
    17641 gaggccgcga acctcgacaa gttcaacgag atcatgcgcg agctcggcca caagcacatc
    17701 cgtgctccgg tgccgcatca cgagtacacg acgcgcaagg tcctcgtcat ggagcgcttc
    17761 agcggcctcc gcgtcgacaa gttcgaggaa atcaaagcca agggcatcga cggcgaaggc
    17821 aagctcgtcg acgggctacg cgcatggttc cagtgcgtgg tgttctacgg cttcttccac
    17881 ggcgacgtgc acgcgggcaa cttgatgctg ctcgacgaca acacgatcgg cttcctcgac
    17941 ttcggcatcg tcggccgctt cgatgacaag gcgcgctacc tcgtcaccga ttacatgatc
    18001 gcgttcgcgt caggtaacta taaacggctc gcggaaatca tcatcgagat ggcgggtgcg
    18061 cccgacggtc tcgacatgga gtcgttcgtc gccgacctcg gcacgacgta ccgtccgctg
    18121 ctcacgctgt cgttcggcga ggtcaactac gccgacttct tgccgggcat tcagcgcacg
    18181 gcgacgcgcc acaagatgcg catgccgaac gagttcattc tgatcacgaa gcagctgctg
    18241 tacttcgatc gctacgcgaa gtcgctggcg ccgaagctga acgtgttcac cgatccgcgc
    18301 ctggtgctcg gcatgatgag cgacatccag aaggcgcgca tggcgcacga agcgaagacg
    18361 aagaacgcaa cggcttagcc gaagcgatcg tcgatgttcg atggcttctt cgagcgcatc
    18421 ggcttctcgt cgccgaggta cttgagaaac agcgtcaggt actcggcgcg ctgcttgtcg
    18481 agtccgcgaa acgacaggcc gacctgaaac gcatcctcga cgggcgtgca ccacacgatg
    18541 cgcacgggca gccgcaacgg ctccgattgc gacgcatcgt cgaacttcag cgtgagctcg
    18601 atctcgacat gcgagccgat cggcagcgcg ttcgcgaggt cggcgcacag gccaccgcgc
    18661 gacacgttct tcgtgcggcc gtccatgact tgcttgccga cgtagaactt gatcccgacg
    18721 tcggcggcgt agcgtggatg ctgacggtcg atactaccca cttacctcaa tggtggccgg
    18781 catcgacggg gagtggcaag tttcccgtcg aggagggtgg gggcacgatc gggtattgaa
    18841 gtgcctttcg tccgcttctc ggacgcaacg catcatcgta gttcagcgcg tttggctagc
    18901 gcctgcgaga accacccagc cgtcagggcg tcgagctcgg cagccttttc ctgagcgtcg
    18961 agctcggcgc ggatgtctgc atcggccgct acccgagccg cgacccagtc gtagtagagg
    19021 tggatcgcct gggcggcgag gcgacgccgc tcgcgccggc gcgcgacatc gagcatcgcg
    19081 atgcgtggct cgtcgcggtc gaacaggagc tggtcgaggc gccagctcgc acgtacgtcg
    19141 atgccgacgc cgtggctgat cgtcggatcg tcgacgtcac gccaggcctg gttctgaccg
    19201 gcgcgcacgc tgaccatcgg cacgagcgcg gcgaggcgcg agcggcgccg ccagctgggt
    19261 gtcggatcgt cggccacgcc acccgcgcga tacgccgccg cgaccacgct cgcgatcggc
    19321 ggcgcgaacg tcgcgatgcg ctcgatcatc gcgttcgtga tcgggacgta gaggtggcgc
    19381 tgcggggatg gccgatccgg gtctcccccg tactcgccgg gaagctcgag cgatgctccc
    19441 ggtggagcgt cgtcctcgtc atcccgtggg gccgcattgg cgcgcgcgac gagcactgcg
    19501 agtagggcaa cgacggcgac gacgatcagc gcgaggtggc cgcgaggctg gttgcgtgcc
    19561 cgggcgacgg atcgtacggt gcgcacgtgt cgacgacgct caccgagaac gcgctgcagg
    19621 tgcttcgcgc ccgctacctc gcgcgggaga acggcgtcgt cgtggagacg cctgagcagc
    19681 tgttccgccg cgtcgctcgc cacgtcagcg ccgtcgaggg cgcgctcggc tacagccccg
    19741 ccgaggtcga cagcgcacgc gccaagttcg agcgaatgat ggtggcgctc gaattcgtac
    19801 cgaattcccc gacgctcatg aatgccggcc gcccactcgg gcagctcgct gcctgcttcg
    19861 tggtcccggt cgacgactcg accaccggcg tattcgaggc cgttcgctgg gccgcggaga
    19921 tccagaagac cggcggtggc accgggttct cgttctcgcg gctgcgcccg gccggcgacc
    19981 tcgtcgcatc gaccggcggc aacgcgtccg gtcccgtatc gctgatgcag gtcttcgatg
    20041 tcgcgaccga ggcgatcaaa cagggcggca cccggcgcgg cgccaacatg ggaatgctgc
    20101 gcgtcgacca ccccgacatc ctcgagttca tcgcgctcaa gctcgatccg acgcggatgc
    20161 gcaacttcaa cctgtcggtc gcggccaccg acacgttcat gaaggcggtc gctgccggca
    20221 cgacctacga cctcgtaaat ccgcgcacga agatcgtcac cggtcagctc gatgcgcggc
    20281 gcgtgttcga tgcgatcgcg aacgccgcgt gggcgtgcgg cgaccccggg ctcgtgttca
    20341 tcgatcgcgt caacgacctc cacccgacgc ccgcgctcgg cccgatcgag gcgaccaacc
    20401 catgcgtcac cggggagtcg ctcgtgtgga cctatgctcg cggcctcgtc ccgatccgcg
    20461 acctcgtcgg cacgcagccg cgggtagcaa cgctcgacgg tgatgaggtc gtgttccgga
    20521 ccgccaccca ggtcgtgtgc accggctatc gcaaggtcta tcgcctgcgc accggcagcg
    20581 gcctgagcct gcggctcacc gccgaccatc gtgtcgcgac cgactgcggc gacgtagctg
    20641 ccgagcacct cgaggtcggc atgcgcgtcc gcgccatcga gaatcatcga gtcggtcacg
    20701 gccacggtcg cgcaatcgct acggcgggca acatgggtcg ccgaccactc cgcgcggacg
    20761 cgctcgacgt tccgcgcgaa cgcctcggcg agctcctcgg ctggaccacc gtcgtcgcgc
    20821 tcgaaccgga cggttgcgag ctcgtctacg acctcaccga gccgacgacc agccacttcc
    20881 tcgcgaacgg gatgctcgtc cacaactgtg gcgagcagcc attgctcccg ttcgagtcgt
    20941 gcacgctcgg ctcgatcgac gtgggccgct tcgtagccgg ccgcgacttc gtttgggagc
    21001 gcctccggga ggccatccac gattccgtcc gcttcctcga
//
LOCUS       DASUFX010000554         5522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1338905, whole genome shotgun sequence.
ACCESSION   DASUFX010000554 DASUFX010000000
VERSION     DASUFX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338905"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..471
                     /locus_tag="VFV99_22200"
     CDS             85..471
                     /locus_tag="VFV99_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062105.1"
                     /translation="MNARRDALAHRFVAFTHDETQAWPADLQVSWHELAFSTLPELIL
                     ELDAPAACRFARSWRERVEAQLATPAPAFDDAELAWLNDIARGYGAAEPVAGTSHDVT
                     RLADLLRRFGPLALSLPWRHTPPAKA"
     gene            577..2391
                     /locus_tag="VFV99_22205"
     CDS             577..2391
                     /locus_tag="VFV99_22205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5062106.1"
                     /translation="MAQRSSLAYLRPYKRGLIAGVVMLIATNLCYLGVAKYLKDAVDA
                     LKNGHQNAVPTLVAWLIGFAIATALTRIVSRVWIFNMARAAEYDLRSELFGHIMTLAP
                     SYYRDHPTGDVMSRLTNDVQTVRAMWGAGLVHLANAVTAFATVLTYMIVLDPMVAALA
                     IIPYPLIFFVGQALSKRVYRTTREVQAELGSLSARIQEDLGAIQVIKTYTLEDVRREG
                     FLAGSQRLLEKNMDAAKVRMQLGPTLNTLAPIAIAILILVGGTAVINKDISVGAYTAY
                     GMLLTRLVWPTLTLGFMLALVQRGRASWTRLVELQDTKTELPDPSGEPLPKRSDPATV
                     EVKGLTFKIGDRALLEDVSFTLAPGTVTAIVGRTGAGKSTLVDAMCRMNNVPAGTIFL
                     DGRDITAIPLASLRAQIGYAPQEAFLFSTTIADNVAMGYGAGTAIPAARRVELERVGA
                     AASAPDLSNEPRITNAAKAAGLARDLAVMPERLATVVGERGITLSGGQRQRVALARAL
                     AAQPRLLLLDDSLSSVDAETERVILAHLREVMAGRTAVLISHRVAAIKDADQILVIDQ
                     GKLVERGTHDELIEKEGLYSELYRTQLDTEMDIVQEAS"
     gene            2391..4238
                     /locus_tag="VFV99_22210"
     CDS             2391..4238
                     /locus_tag="VFV99_22210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5062107.1"
                     /translation="MAVAAPPRKSGLEAEAVLGKAYDLRLVRRLSKYIKAHFHLLVGW
                     VVFMFVTIGFQLAQPYLIMYTLQHHIMTGKASALPWDALLFVGLVVGQNVSSFIEQWL
                     LQLAGQRTMHDLRIAIFDHVLAQRAAFFDRIPVGRLMTRMTNDIESLNEMFAQGAITL
                     IADAVKAAVIVVIMLTIDVELSLMTLATLPLLFVLVEYARRAMRKSFRQIRVRLAAMN
                     AFAQEHLFGIRVVHLLGRGTIAQQEYDEINAGHRDAYLLQIRADASMYALVEAIATIA
                     IGLIVWWASGHRIQDAATIAIVVGFIEYSKMFFEPIRDLSAKYAVMQGAMAASERIFQ
                     LLDTEEYDGGVSPAGRSESAPLHSGASSTGRDPRAKPAPTTSAVELADVHFSYGAEPV
                     LRGVDLHVPRGATVAVVGATGSGKSTIIKLLTRLYERDKGAIKIDGLDVRDIPLDELR
                     ERITVVSQDVILFAGSLADNIALGKAYDRARLDAAVKRVGLDRALVRRGADVTAKVSE
                     RGSNFSAGERQLVAFARALLRDPEILVLDEATAHVDPEAEELIERGVQELMKGRTTLV
                     IAHRLSTIRNADLIVVVDKGQVVEQGTHDELVAKGGLYAALEKTFRRQD"
     gene            complement(4240..4416)
                     /locus_tag="VFV99_22215"
     CDS             complement(4240..4416)
                     /locus_tag="VFV99_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062108.1"
                     /translation="MRASRESIDAFGAELVMRRPPPWRWSYSQWTGYWYGPLPIGAVL
                     AVVAGLLFGELVLL"
     gene            4539..>5522
                     /locus_tag="VFV99_22220"
     CDS             4539..>5522
                     /locus_tag="VFV99_22220"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062109.1"
                     /translation="MSAGMYERLRIAVDVMADGAIVADTRTGEVTINRAAREMLGIGA
                     DEPVDTAYLKDVVGFYPFDLAAGAADDQPIREEVRIGERVLHSVVTPLREGDTPIGAI
                     VVLRDLGDTADVARRRAEFAQVMSHELRTPLTSIAGALDIVLSGYAGPLTDRQLRYVD
                     MARQAATRMNQLVDQLLDLARAQAGSITVSTAPVQLDRLAREVIDRYRAAAATKQLTI
                     VLGASADDISILGDPERLAQVLGNLITNAIRFAPANGVIDVQVFGPPLSEDAVGVSVH
                     NSGDPIAPEDHERIFEPFSASSRRVGGTALGLSISRTIIEAHGGRIWVESGA"
BASE COUNT          930 a         1919 c         1787 g          886 t
ORIGIN      
        1 cggcaagaaa gcacaggaca tcgaggacga gcgcgaagag aggaaattcc gggagtcgct
       61 cggcgtcaaa ccgtacaagc gcccatgaac gcccgccgcg acgccctcgc ccaccgcttc
      121 gtcgcgttca cgcacgacga gacccaggcg tggccggcgg acctccaggt cagctggcac
      181 gagctcgcgt tctcgacgct gcccgagctc atcctcgagc tcgacgcgcc agcagcctgc
      241 cgcttcgcgc gctcgtggcg cgaacgcgtc gaggcccagc tcgcgacgcc ggcgcccgcg
      301 ttcgatgatg ccgagctcgc gtggctgaac gacatcgcgc gcggctacgg tgcggccgag
      361 ccggtcgcag gcacgagtca cgatgtcacc cgccttgccg atttgctgcg caggttcgga
      421 ccgctcgcgc tctcactacc gtggcggcac acgccacccg caaaggcctg acgtcaaatc
      481 cggttgcctg gttacaactc gatcccgacg gcgcaaagtg ttgccgtcgg gacggttgtg
      541 gagcctcttc agggagggta gacaggtaac tcagtcgtgg cgcagcgcag ctcactcgcc
      601 tacctccggc cgtacaagcg ggggctgatt gccggcgtcg tgatgctgat cgcgacgaac
      661 ctctgctacc tcggcgttgc gaagtatctg aaggacgcgg tcgacgcgct gaagaacggc
      721 caccagaacg cggtgccaac cctcgtggcc tggctgatcg gcttcgcgat cgcgaccgcg
      781 ctcacccgca tcgtgtcgcg cgtgtggatc ttcaacatgg cgcgcgcggc cgagtacgac
      841 ctgcgcagcg agctgttcgg ccacatcatg acgctcgcgc cgtcgtacta ccgcgaccac
      901 ccgaccggcg acgtgatgtc gcggctcaca aacgacgtcc agaccgtgcg tgcgatgtgg
      961 ggcgccgggc tcgtccacct cgcaaacgcg gtgacggcgt tcgcgaccgt gctgacgtac
     1021 atgatcgtgc tcgacccgat ggtcgcggcg ctcgcgatca ttccctaccc gctcatcttc
     1081 ttcgtcggcc aggcgctctc gaagcgcgtc tatcgcacga cgcgcgaagt gcaggccgag
     1141 ctcggctcgc tgtcggctcg catccaggag gacctcggcg cgatccaggt gatcaagacg
     1201 tacacgctcg aggacgtgcg ccgcgaaggg ttcctcgcag gcagccagcg gctgctcgag
     1261 aagaacatgg acgcggcaaa ggtccgcatg cagctagggc cgaccctgaa cacgctcgcg
     1321 ccgatcgcga tcgcgatctt gatcctggtc ggtggcaccg cggtgatcaa caaggacatc
     1381 agcgtcggcg cgtacacggc ctacggcatg ctgctcacgc gcctcgtgtg gccgacgctc
     1441 acgctcggct tcatgctcgc gctcgtccag cgcggccgcg catcgtggac ccgcctcgtc
     1501 gagctccaag acacgaagac cgagcttccc gatccgtccg gcgagccgct gccgaagcgc
     1561 agcgatcccg ccaccgtcga ggtgaagggg ctgacgttca agatcggtga ccgcgcgttg
     1621 ctcgaggatg tcagcttcac actcgcgcca ggcacggtta cagcgatcgt cggccgcacc
     1681 ggcgccggca agagcacgct cgtcgacgcg atgtgccgca tgaacaacgt gccggcaggg
     1741 acgatcttcc tcgatggccg cgacatcacg gcgatcccgc ttgcgtcgtt gcgcgcacag
     1801 atcggctacg cgccgcagga agcattcttg ttctcgacga cgattgccga taacgtcgcg
     1861 atgggctacg gcgccggcac tgcaatcccc gcggcccggc gggtcgagct cgagcgcgtc
     1921 ggtgctgccg cctcagcgcc agatctatcg aacgaaccgc ggatcacgaa tgccgcgaaa
     1981 gccgccgggc tcgctcgcga cctcgccgtg atgccggaac gcctcgcgac cgtcgtcggc
     2041 gaacgcggca tcacgctctc gggtggccag cgccagcgcg tcgcgctcgc gcgcgcgttg
     2101 gccgcacagc cgcgactgct gctgctcgac gactcgctgt cgtcggtcga cgccgagacc
     2161 gagcgcgtga tcctcgcgca cctgcgcgag gtgatggccg gccgcacggc agtgctgatc
     2221 tcgcatcgcg tcgctgcgat caaagacgcc gatcagatcc tcgtgatcga tcaaggcaag
     2281 ctcgtcgagc gcggcacaca cgacgagctg atcgagaagg aaggcctcta cagcgagctc
     2341 taccgcaccc aactcgacac cgagatggac atcgtgcagg aggcctcgta atggcggtcg
     2401 cggcgccccc acgcaagagc ggcctcgaag ccgaggcggt gctcggcaag gcgtacgact
     2461 tgcggttggt ccgccggctg tcgaagtaca tcaaggcgca cttccatctg ctcgtcggct
     2521 gggtcgtgtt catgttcgtc acgatcgggt tccagctcgc gcagccgtac ctgatcatgt
     2581 acacgctgca gcaccacatc atgaccggca aggcgagcgc gctgccgtgg gatgcactgc
     2641 tgttcgtcgg cctcgtcgtc ggccagaacg tgtcgtcgtt catcgagcag tggctgctcc
     2701 agctcgctgg ccagcgcacg atgcacgatc tccgcattgc gatcttcgat cacgtgctcg
     2761 cgcagcgcgc cgcgttcttc gatcggattc cggtcggccg gctgatgacg cgcatgacga
     2821 acgacatcga gagcctcaac gagatgttcg cgcaaggcgc gatcacgttg atcgccgacg
     2881 ccgtcaaagc ggccgtcatc gtcgtcatca tgctgaccat cgacgtcgag ctgtcgctga
     2941 tgacgctcgc gacgctgccg ctgttgttcg tgctggtcga gtacgcgcgc cgcgcgatgc
     3001 gcaaatcgtt ccgccagatc cgcgtgcgac tcgcggcgat gaacgcattc gcgcaagagc
     3061 acctcttcgg catccgggtc gttcacctgc tgggccgcgg cacgatcgcg cagcaggagt
     3121 acgacgagat caacgcgggc catcgcgatg cgtatctgct gcagattcgc gccgacgcct
     3181 cgatgtacgc gctcgtcgaa gcaatcgcca cgatcgcgat cggcctcatc gtgtggtggg
     3241 cgagtggcca tcgcatacag gacgccgcga cgatcgccat cgtggtcggc ttcatcgagt
     3301 acagcaagat gttcttcgag cccatccgcg acctgtccgc gaagtacgcc gtcatgcagg
     3361 gcgcgatggc ggcgagcgag cgcatcttcc agctcctcga caccgaggag tacgacggcg
     3421 gcgtctcccc cgcgggccgg agtgaatccg ccccgctcca ctctggggcc agctcgaccg
     3481 gccgcgaccc gcgtgcgaag cccgcgccga cgacatcggc cgtcgagctc gccgatgtgc
     3541 acttcagcta cggcgccgag ccggtgctgc gcggcgtcga tttgcacgtc ccgcgtggcg
     3601 cgaccgtcgc ggttgtcgga gcgactggct ccggcaagtc gacgatcatc aagctgctca
     3661 cgcgcctata cgagcgcgac aaaggcgcga tcaagatcga cggcctcgac gtgcgcgaca
     3721 tcccgctcga cgagctgcgc gagcggatca ccgtggtctc gcaggatgtc atcctgttcg
     3781 cagggtcgct cgccgacaac atcgcgctcg gcaaggcgta cgaccgcgcc cgcctcgacg
     3841 cagcggtgaa gcgcgtcggc ctggaccgcg cgctcgtacg ccgcggcgcc gacgtgaccg
     3901 ccaaagtctc cgagcgcggc tcgaatttct ctgctggcga gcgccagctg gtcgcgttcg
     3961 cacgcgcgtt gctccgtgac cccgaaatcc tcgtgctcga cgaggcgacc gcgcatgtcg
     4021 atcccgaggc ggaggagctc atcgagcgcg gcgtgcagga gctgatgaaa ggccgcacga
     4081 cgctcgtcat cgcgcaccgg ctgtcgacga tccgcaatgc ggatctgatc gtcgtcgtcg
     4141 acaagggtca ggtcgtcgag caaggcacgc atgacgagct cgtcgccaag ggcgggctct
     4201 acgccgcgct cgagaagacg tttcgccgcc aggactgagt cagagcagga cgagctcgcc
     4261 gaacaagagc cccgcgacga cggcgagcac cgcgccgatg ggcagcggtc cataccagta
     4321 gccggtccac tgcgagtacg accaccgcca cggaggcggt cgccgcatga cgagctcggc
     4381 accaaatgca tcgatcgact ctcgacttgc gcgcatggtg gcgctctcgg tcaacccgcg
     4441 gcccggttgc gagctcttag cagcccgaac gggcggctac atgtcgtacc cctctcggct
     4501 aggccacttg gacgtggttt aggtatcatt ctttacgaat gtcagccggg atgtacgaac
     4561 gactgcgaat cgcggtcgac gtcatggcgg acggcgcgat cgtcgccgac acgcgcaccg
     4621 gcgaagtcac gatcaatcgc gccgcacgcg agatgctcgg cattggggcc gacgaaccgg
     4681 tcgacaccgc gtacctcaag gacgtcgtcg ggttctatcc gttcgacctc gcggccggcg
     4741 ctgctgatga ccagccgatt cgcgaagagg ttcgcatcgg cgagcgcgtg ctccactcgg
     4801 tcgtcacccc gctacgcgaa ggcgacacgc cgatcggcgc gatcgtcgtg cttcgcgatc
     4861 tcggcgacac cgccgatgtc gcgcgccgcc gcgccgaatt cgcccaggtg atgtctcacg
     4921 agctgcgtac gccgctcacg tcgatcgccg gtgcgctcga catcgtgctg tcgggctacg
     4981 cgggcccgct cacagatcgc cagctccgct acgtcgacat ggcgcgccaa gcggcgacgc
     5041 ggatgaacca gctcgtcgac cagctgctcg acctcgcgcg tgcgcaggcc ggctcgatca
     5101 ccgtgtcgac ggcgccggtg cagctcgatc gcctcgcgcg cgaagtcatc gatcgttacc
     5161 gcgcagctgc agcgacgaag cagctcacga tcgtgctcgg cgcatcggcc gacgacatct
     5221 cgatcctcgg cgaccccgaa cgcctcgcgc aggtgctcgg aaatctaata acgaacgcga
     5281 tccggttcgc gccggcgaac ggcgtgatcg acgtccaggt gttcggcccg ccgctgtccg
     5341 aagacgcggt cggcgtctcc gtccacaact ccggtgatcc gatcgcgccc gaagatcacg
     5401 agcgcatctt cgagccgttc tcggcgtcga gccggcgcgt cggcggcacc gcgctcggcc
     5461 tgtcgatctc gcgcacgatc atcgaggcgc acggcggccg gatctgggtc gagtccggcg
     5521 cc
//
LOCUS       DASUFX010000555         5252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_228810,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000555 DASUFX010000000
VERSION     DASUFX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228810"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFV99_22225"
     CDS             complement(<1..1051)
                     /locus_tag="VFV99_22225"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062110.1"
                     /translation="MRIGELLVEQRKLRANDLARALAEKPSDKRLTSFLIAKGLVDFD
                     DASRALGEQRGFACALAKHIAGRDPELARLIPPDLGRASCVLPIGKSSRGAIIVCARD
                     PAPALLAQLQAAIRGDVLMVIAPATRLENLIAEAYGKSSTGEFDIDFDLANDLPAPTP
                     SRVTRGPLPTPPGPFDAVDPTQTLATPGSPNQYPRAETPAPLPPLPDMAALDPESIRY
                     SLTSLDDYRVTKDHGQSGSIPIRGGTLPPTNKPITTPPLNVLAGSPTTISRTTRDVPP
                     APTKDLKPPTSPPAPTSSLAPGTYPPKRTNVLDAVSPPAPSGDLNPPTPRPGSALNPP
                     TSPPKRTNALDAAGPP"
     gene            complement(1103..1291)
                     /locus_tag="VFV99_22230"
     CDS             complement(1103..1291)
                     /locus_tag="VFV99_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062111.1"
                     /translation="MPKQPTKQPAPRKKSDETNRHAPEREDVEAELPQAPAKEPLAEP
                     VPLDGARAPRPTVEAEVE"
     gene            1342..1968
                     /locus_tag="VFV99_22235"
     CDS             1342..1968
                     /locus_tag="VFV99_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062112.1"
                     /translation="MSLFVVIAVTATVIGLVGGIPDPRKVKRELARARVQTIDSLVEG
                     KPGAVRGTVAVIDPETTIWAPLTQHRCVYVLVVFDEVGVGGDYRELGRIEGTASFLLR
                     SEHGTARIVPSGARLALPGHSVARPFMNLGPMLTELSRGQIEQPNYPTSWLRATEYRI
                     DEGEELTIRGWVTREPLPEASDAVSGYREQLPTRAVISGSRRAKLLIG"
     gene            complement(1987..3441)
                     /locus_tag="VFV99_22240"
     CDS             complement(1987..3441)
                     /locus_tag="VFV99_22240"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062113.1"
                     /translation="MNAHYVEKHIGQYKILRTLGAGGMGTVYLGEHLLLGRRAAIKTL
                     LPALSSHREIVDRFFNEARAISAISDPGVVQVFDFGYHVDGTAYIVMELLEGESLAAR
                     LTRLGTLPLVEALRLARQLSSSLDAAHRRDVVHRDLKPGNVFVIRDSEVQGGERAKIL
                     DFGICKLGDPEHPMTTQTGAMLGTPVYMSPEQCRGAGDVDHRSDIYSLGCVLFHMLTG
                     RPPFDCEGGGEYIASHLKEEPPAPTTIAPELPPAIDALMALCLAKDPDRRFQSMAELQ
                     QAIELVLAQITETDSVAVAEPVLLKTPLGEGFQSAYDGNFTNRPPTPPAHSWFVDSLL
                     PVSISDHYEVPRPRRWGRRLAYMLALVLGVVSGVAGTGYALDNEIISDVTAPPGPDAT
                     TPEELTPAYLDHAFEVAPADPIAIADAPHAPSVVAKQHIVAKQHAVVRPQLERGEAAR
                     VKRPRAITKPQPPRTRSVVRKALPMPQPEDPYGQ"
     gene            3594..>5252
                     /locus_tag="VFV99_22245"
     CDS             3594..>5252
                     /locus_tag="VFV99_22245"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX5062114.1"
                     /translation="MSLSLLAAVVIAVLAIGYRSYGRFIARQFALSSTPDTPAHTKND
                     GVDFVPTRPFYLFGQHFSAIAAAGPIVGPILACQQFGWLPALLWITIGVIFIGAVHDF
                     TTLVASVRHDARSIAEVVKHTLGPKAWLAILAFIWLALVYVIVAFVDVTASTFVTGDE
                     DAVGLTFRFNQGGAVALAGTLYLVLAVVMGLFERFVKPPLWLSTVIFVPATFGMVWLG
                     TQFSTLILLDWHTWVILIMAYCAIASIVPMWILLQPRGYLGGFVLYAALLVGTLGIFF
                     GGFEIKRPAFVAFDVGGPTGLLFPFLFVTIACGACSGFHGLVCSGTTSKQIDREAHCE
                     PIGYGAMLLEAFVAVIALATVMIVAPDSKQGANAIYASGIGAFIHKLLGLDPYIATTF
                     GAMALSTFIFDTLDSATRLGRYILQELFGRTDRVSGVAATLLTVGVPMSFLLAGKQGS
                     YKLFWTLFGTSNQLLASLSLLAITVWLKKLGKPIWFTLAPMVLVFSVTVVSLTLQIKQ
                     LTHAAVGTAPWLNGLVSLILLGLALILVGYAARAWRGMPSAGMQG"
BASE COUNT          771 a         1685 c         1793 g         1003 t
ORIGIN      
        1 cgggtggtcc ggctgcatcg agcgcgttcg tgcgtttcgg cgggctcgtt ggtgggttca
       61 gcgcgctgcc cggccgtggc gttggcggat tgagatcacc gctcggtgcc ggcgggctca
      121 ccgcgtcgag cacgttcgta cgcttcggcg gatacgtgcc aggcgcgagc gagctggtcg
      181 gtgccggcgg gctcgtcggt ggcttcagat ccttcgtcgg cgccggcggc acgtcccgtg
      241 tcgtgcgcga gatcgtcgtc ggcgaaccgg cgagcacgtt gagcggaggc gtcgtgatcg
      301 gcttgttcgt cggtggcaac gtgccgccgc ggatcggaat cgaaccgctc tggccgtgat
      361 ctttcgtcac gcgatagtcg tcgagcgacg tgagcgagta gcgaatgctt tcgggatcga
      421 gtgccgccat gtcgggcagc ggcggcagcg gcgccggcgt ctctgcgcgc gggtactgat
      481 tcggcgaccc gggtgtggct agtgtctgtg tcggatcgac cgcatcgaag gggcccggtg
      541 gcgtcggcag cgggccacgc gtcacgcgcg acggtgtcgg cgcgggcaga tcgttcgcga
      601 gatcgaagtc gatatcgaat tcgccggtcg atgatttgcc gtacgcctct gcgatgaggt
      661 tctcgagccg cgtcgccggt gcgatgacca tcaggacgtc gccgcggatc gcagcctgca
      721 actgcgcgag cagcgccggc gcggggtcgc gcgcacacac gatgatcgcg cccctgctcg
      781 acttgccgat cggcaacacg cacgacgcgc ggccgaggtc gggcgggatc aaccgtgcaa
      841 gctccggatc gcggcccgcg atgtgtttcg cgagtgcaca cgcgaagccg cgttgttcac
      901 cgagtgcgcg cgatgcgtcg tcgaaatcga cgagcccctt cgcgatcaag aacgatgtca
      961 atcgcttgtc ggacggcttc tcggcgagcg cgcgcgcgag gtcattcgcg cgcagcttgc
     1021 gctgctcgac caggagctcc ccaatgcgca tccggggatc catcttgcca tgaccacatg
     1081 tgcgacatgc cagcgaaccg gctcattcga cttccgcttc gacggtagga cgtggtgcgc
     1141 gggcaccatc gagcggcacc ggttcggcga gcggttcctt cgcgggcgcc tgcggtagct
     1201 cggcttcgac gtcctcacgc tcgggagcgt gccggttcgt ctcgtccgac ttcttcctcg
     1261 gtgcgggctg tttcgtgggc tgttttggca tcacgaatcc tccgagatcc acaggtgcac
     1321 gagtcgtgac aaggtacgca cgtgagcttg ttcgttgtca ttgcggtgac cgccaccgtg
     1381 atcgggctcg tcggaggcat accggatccg cgcaaagtga aacgcgagct tgcgcgtgct
     1441 cgcgtacaga cgatcgattc gctcgtcgaa ggtaagcctg gcgctgtacg cggcaccgtt
     1501 gccgtcatcg atcctgaaac gacgatctgg gcgccgctga cgcagcaccg ctgtgtctac
     1561 gtcctcgttg tcttcgacga agtcggcgtc ggcggcgact accgcgagct cggccggatc
     1621 gagggcaccg cgtcgttctt gctgcgctcg gagcatggca ccgcgcgtat cgttccgagt
     1681 ggagcccgcc tcgcgttgcc gggccactcc gtcgcgcgac cgttcatgaa tctcggcccc
     1741 atgctcaccg agctctcgcg tggtcagatc gagcagccga actatccgac gtcgtggttg
     1801 cgcgccaccg agtaccggat cgatgagggc gaagagctca cgatccgcgg ctgggtcacg
     1861 cgcgagccgc tgcccgaagc gagcgacgca gtgtcgggct atcgcgagca gctgccgacg
     1921 cgcgccgtaa tctcgggctc gcggcgcgcg aagctgctga tcgggtagcg cgaacgcgct
     1981 cgatcgctac tgcccgtaag gatcctcggg ttggggcatc ggtagcgcct tgcgcacgac
     2041 cgatcgcgtg cgcgggggct gtggcttcgt gatggcgcgt ggtcgtttca cgcgtgcagc
     2101 ttcgccccgc tcgagctgcg gcctcacgac tgcgtgctgc ttcgcgacga tgtgctgctt
     2161 ggcgacgacg gacggtgcgt gcggtgcatc ggcgattgca atcggatcgg ccggcgcgac
     2221 ttcaaacgcg tgatccaggt atgccggcgt cagctcttct ggggtcgtcg catcgggacc
     2281 gggaggcgcg gttacgtcgc tgatgatctc gttgtcgagc gcgtagcccg tgccggcgac
     2341 accgctgacg acaccgagga cgagcgccag catgtacgcg aggcgacggc cccagcgccg
     2401 cggccgtggg acctcgtagt gatcggagat cgaaaccggc agcagcgaat cgacaaacca
     2461 cgaatgcgct ggcggcgtcg gcgggcggtt cgtgaagttg ccgtcgtacg cggattggaa
     2521 tccttcgccg agcggcgtct tcaggagcac cggctctgcc accgcgacgc tgtcggtctc
     2581 ggtgatctgc gcgagcacga gctcgatcgc ctgctgcagc tcggccatcg attgaaagcg
     2641 gcggtccgga tccttcgcga ggcacagcgc catcagcgcg tcgatggccg gcggtagctc
     2701 gggggcaatc gtcgtcggcg ccggtggttc ctccttgagg tgcgacgcga tgtactcgcc
     2761 gccgccctcg cagtcgaacg gcggccgtcc ggtgagcatg tgaaacagca cgcagccgag
     2821 cgaatagatg tcggagcgat gatcgacgtc gccggcgccg cggcactgct ccggtgacat
     2881 gtagaccggc gtgccgagca tcgcgccggt ctgcgtcgtc atcgggtgct cgggatcgcc
     2941 gagcttgcag atgccgaagt cgaggatctt cgcgcgctcg ccgccttgca cctccgagtc
     3001 gcggatcacg aacacgttgc cgggtttgag atcgcgatga acgacgtcac gccggtgggc
     3061 ggcatcgagc gagctcgaca gctggcgcgc gaggcgcagc gcctcgacca gcggcagcgt
     3121 tccaagtcgc gtgaggcgcg ccgcgagcga ctcgccttcg aggagctcca tcacgatgta
     3181 cgcggtgcca tcgacgtggt agccgaagtc aaagacctgc acgacgccgg gatcggagat
     3241 cgccgagatc gcacgcgctt cgttgaagaa gcgatcgacg atctcgcgat gactcgatag
     3301 cgcgggcagg agcgtcttga tcgccgcgcg ccggccgagc aacagatgct cgccgagata
     3361 gacggtgccc attccgccgg cgccgagcgt acgcaggatc ttgtactgac cgatgtgctt
     3421 ctcgacgtaa tgcgcgttca cgtgcagaag gtgtagcgcg ccggcgggaa ttgcctaatg
     3481 aacatccgca cttgagtcac catggctcgc tgtgcccggc tgaccgagcg ccgttgtcaa
     3541 tcgcgtaccg ggagccgcgt ggatcgtagc gatccgcctc tatcatgcgg gcgatgtcac
     3601 tctcgttgct cgcggccgtc gtgatcgcgg tgctcgcgat cggctatcgc tcgtacggcc
     3661 gattcatcgc gcgccagttc gcgctctcat cgacacccga tacaccggca cacacgaaga
     3721 acgacggcgt cgatttcgtg ccgacgcgtc cgttctatct gttcggtcag cacttcagcg
     3781 cgatcgccgc ggcgggaccg atcgtcgggc cgatcctcgc gtgtcagcag ttcggttggt
     3841 tgcccgcgct gctctggatc acgatcggcg tgatcttcat cggcgccgtc cacgatttca
     3901 cgacgctcgt cgcatcggta cgccacgacg cgcgctcgat cgctgaagtc gtcaagcaca
     3961 cgctggggcc aaaggcatgg ctcgcgatcc tcgcgttcat ctggctcgca ctcgtctacg
     4021 tgatcgtcgc gttcgtcgac gtcacggcga gcacgttcgt caccggcgat gaagacgccg
     4081 tcgggctcac gttccgcttc aatcaaggtg gcgcggtcgc gctcgccggg acgctctacc
     4141 tggtgctcgc agtcgtgatg ggcctgttcg agcggttcgt gaagccgccg ctgtggctgt
     4201 cgaccgtgat cttcgtgccg gcgacgttcg gcatggtgtg gctcggtacg cagttctcca
     4261 cgctgatcct gctcgactgg cacacgtggg tcatcttgat catggcgtac tgcgcgatcg
     4321 cgtcgatcgt gccgatgtgg atcttgttgc agccgcgcgg ctacctcggc ggcttcgtcc
     4381 tgtacgcagc gctgctcgtc ggcacgctcg gcatcttctt cggcggcttc gagatcaagc
     4441 ggcccgcgtt cgtcgcgttc gatgttggcg gaccgaccgg cctgttgttc ccgttcttgt
     4501 tcgtcacgat cgcgtgcggt gcgtgctccg gcttccacgg gctcgtctgc tcgggcacga
     4561 catcgaagca gatcgatcgc gaagcgcact gcgagcccat cggatacggc gcgatgttgc
     4621 tcgaggcgtt cgtcgcggtg atcgcgctcg cgaccgtgat gatcgtcgcg ccggacagca
     4681 agcagggcgc aaacgcgatc tacgcgagcg gcatcggcgc gttcatccac aagctactcg
     4741 ggctcgatcc ctacatcgcg accacgttcg gtgcgatggc gctatcgacg ttcatcttcg
     4801 acacgctcga ctcggcgacg cgcctcggcc gctacatcct gcaggagctg ttcggtcgta
     4861 ccgatcgcgt gtccggtgtc gccgcgacgc tgctcaccgt cggcgtgccg atgtcattcc
     4921 tcctcgcggg caagcagggc agttacaagt tgttctggac gctgttcggc acgtcgaacc
     4981 agctgctcgc gtcgctgtcg ctgctcgcga tcacggtgtg gctcaagaaa ctcggcaaac
     5041 cgatctggtt cacgctcgcg ccgatggtgc tcgtgttctc ggtgaccgtg gtgtcgttga
     5101 cgctgcagat caagcagctc acccacgcgg cggtcggtac cgcgccgtgg ctcaacggtc
     5161 tcgtgtcgct gatcctgctc ggcctcgcgc tgattctcgt cggctacgcc gcgcgggcgt
     5221 ggcgcggaat gccatcggca ggcatgcagg gt
//
LOCUS       DASUFX010000556         6745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_367004,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000556 DASUFX010000000
VERSION     DASUFX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367004"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VFV99_22250"
     CDS             complement(<1..1324)
                     /locus_tag="VFV99_22250"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5062115.1"
                     /translation="MKNLVAIAIVLSFVGVAAAQPAQPVTSVPAVPTAPAPAPAKKAA
                     PKKKAPKADAPKEGPVDPYADAAPQDPYGDVGKKDAKADVPPPDAKKDAKKDAKKDAK
                     ADPKGAKTDPGKPVDPYAAPAVDKDAPPQIPTRVGLSDLPAVQGLLAVQRLDAWLLYD
                     RDGSNPIASRLVKPDGRPQRPWFYLIPAKGDPTVLVHTSEARNFEHLAGKKLTYVGYR
                     DLDKQMRAMLKGVKSAAIEYSPKAAVPNVSRVDAGTVENIRAAGVQVRSSETLVQYTK
                     AIWGDAGRTAHYVAVHHVVELRKEALAFVAKQLRAKQPVSEYDVQQRLMHNMTMRGLV
                     GPPPVVAAGVNTADPYYVPTAAKTAQINPGDVIVIGIAAKLDKPDGIYAAQTWVAVAD
                     KAVPENIAKAFEQASLARDQALVLITDRSRKRRPVTGAEVDQATRAFMK"
     gene            complement(1321..2091)
                     /locus_tag="VFV99_22255"
     CDS             complement(1321..2091)
                     /locus_tag="VFV99_22255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5062116.1"
                     /translation="MSDLGELLAIGSRAARAAGELLRTARPEHIRGKSTAKDLVTEWD
                     VRSEETIRAILEPTGIAVMGEEKDGTRRGDTHWIVDPIDGTVNFAHGLPLWCVSIALE
                     QAGELTVGVVYAPLLDWWFEATRGGGARDGAGTVLHVSECPSLDKAMLATGFPYIATT
                     AENYAEWEHFSRAATCRRLGAAALDLCLVARGWFDGYWERDLQAWDMAAGALIVVEAG
                     GTVTNTLGARFDVHSGEAVASNGAIHSELITELAKVRK"
     gene            complement(2088..3965)
                     /gene="murC"
                     /locus_tag="VFV99_22260"
     CDS             complement(2088..3965)
                     /gene="murC"
                     /locus_tag="VFV99_22260"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5062117.1"
                     /translation="MKVHLIGIGGTGMGAVAGLLAAAGHDVRGSDAAVYPPMSEQLKA
                     LGVPVMMPYAPENLDWGPELVVVGNVHGKDHIEVRAAQERGIKLTSFPAVLGERLLDA
                     KHSIVVSGTHGKTTTTSLIAHILQEAGRDPSLFVGGVPVSLGQGWRLGQGPEFVIEGD
                     EYDTAFFDKGPKVAHDRPRTAVLTSVELDHVDIFPSFDAVRDTFKKLIALIPQDGLLV
                     VCAESPDARAVAKYAACKVEGYAVLGGGESGAEFDVDTESGIASDVAWWARNLEIQKS
                     GRVAFDVYHNGERYEHFESLLVGRHNVMNCVAAIAVAHSRGVPISAIQRGVSSFAGVR
                     RRQELRGIAGGVTVLDDYAHHPTAVRETLKALRRRFPKRRLIAVYEPRSATSRRKTFQ
                     HEFADAFSHADEVIVGRLFDPNKIPVEDRFDPEKLALDLHRSGTKGSYTPEVGAIVKQ
                     LAESAAPGDVVCVLSSGSFDGLHDKLLDEIGDPLKPAHLGDMAAVRALLGRVGLAEEP
                     ARDEQFPSFYVLTNEQGMVGSVALDVMGDDAVLRALAVDEECRGAGYGWMLADMAVSQ
                     ARWRGVRRIYLLTESASDFFAAKFGFRVVDRSTLSKQVAASETFTAPKGAGLVAMRLD
                     L"
     gene            4049..4390
                     /locus_tag="VFV99_22265"
     CDS             4049..4390
                     /locus_tag="VFV99_22265"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5062118.1"
                     /translation="MEGQRHERRQYFRGKARPDRVLQVRYRTTLHPSWIAAETRNIGV
                     GGAFIASADVQPVGTAMTLELSLPTSDQVFTLPAIVRWARDQEGMGVQFVGVDVDILL
                     ELNDYFSSLTT"
     gene            complement(4383..5183)
                     /locus_tag="VFV99_22270"
     CDS             complement(4383..5183)
                     /locus_tag="VFV99_22270"
                     /inference="COORDINATES: protein motif:HMM:NF045440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HEX5062119.1"
                     /translation="MTVEELLTALWRDYVATTPQAERIHRLLAARGERVQNDHVALRT
                     YGLPGIGIDALAKPFEALGWRMQPDRYRFDDKKLVARYWQHPDPALPKVFISELCVSE
                     LSAAAYQIIDGLVAQLPAGFGARADLPWAGRPWRVRRAEYESLLIESEYAAWVGAFGF
                     RVNHFTVLVNALTTFPDLQSLNAFLIDHGFTLNESGGVIKGTPAQRLEQSSTRADSIE
                     VDLAGDITRVPSCYYEFARRYPTPSGDLFHGFVPASADKIFESTDVIR"
     gene            complement(5180..6145)
                     /locus_tag="VFV99_22275"
     CDS             complement(5180..6145)
                     /locus_tag="VFV99_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062120.1"
                     /translation="MRALIIVPFLIAASPAPAHADDTFEAKAQNAVRISRVESLVWAF
                     TAPCDAGDDTQQRQCRTVRNTRAAQLAGSTLMIDADKDAFDAGAWSAQKKSLPLTLSG
                     CIRCAGVEIDGKTFYVVTDKDLTSPKFAGGKFKTAPIGDSAKQVADEAAAKTFARSVS
                     NARVQLLVKVPAKPLTTIDGKQVVALDLVGYRVFAPCDGSIVLANPQSGPAEADKKQC
                     GPVVAAASASAEPKLDQLTPKLISEAMKPVTEAAQDCFKKFAVSGSAKLKLQVAGDGS
                     VLKYDQQGDFVNTPTGQCIDEAVKKATFPASKKAKTSINFPLMLQ"
     gene            complement(6145..6681)
                     /locus_tag="VFV99_22280"
     CDS             complement(6145..6681)
                     /locus_tag="VFV99_22280"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX5062121.1"
                     /translation="MGHIDVFKSWAETIRQDTDAFKALLESTKADDKSRKLAAGALQY
                     LVSKMDLIPDWNEGIGVIDDVMVLRVCAALTQDHARGDLPTAVEASLERMANQSEKIS
                     AFLGGPLYDKLKAYCAKVGETPVRGITTAQLLTEDSARKSLYTALKEELEKTVPIVIN
                     DPTDAELRLKAYLTHKLQ"
BASE COUNT         1053 a         2257 c         2250 g         1185 t
ORIGIN      
        1 tcttcatgaa cgcgcgggtc gcctgatcga cctcggcgcc agtgaccggg cggcgcttgc
       61 ggctgcgatc cgtgatcagc acgagcgcct ggtcgcgcgc gaggctggcc tgctcgaacg
      121 ccttcgcgat gttctcggga accgccttgt cggcgaccgc gacccacgtc tgcgccgcgt
      181 agataccgtc gggcttgtcg agcttcgccg cgatgccgat cacgatgacg tcgccggggt
      241 tgatctgcgc ggtcttcgcc gcggtcggca cgtagtacgg gtcggcggtg ttgacgccag
      301 cggcgacgac gggcggcggt ccgacgagac cgcgcatcgt catgttgtgc atgagccgct
      361 gctggacgtc gtactcgctg accggctgtt tcgcgcgcag ctgcttggcg acgaacgcga
      421 gtgcttcctt gcggagctcg acgacgtgat gaacggcgac gtagtgcgcc gtgcggccag
      481 catctcccca gatcgctttg gtgtactgga cgagcgtctc gctcgagcga acctgcacgc
      541 ccgcggcgcg gatgttctcg acggtgccgg cgtcgacgcg cgacacgttt ggcacagcgg
      601 ccttcggcga gtactcgatc gccgccgact tcacgccctt gagcatcgcg cgcatctgct
      661 tgtcgagatc gcggtagccg acgtaggtga gcttcttgcc ggcgaggtgc tcgaagttgc
      721 gcgcctccga cgtgtgcacg agcaccgtgg ggtcaccctt cgccgggatc agatagaacc
      781 acgggcgctg cgggcgaccg tcgggcttca cgagccgcga cgcgatcggg ttggagccat
      841 cgcgatcgta gagcagccag gcgtcgagcc gctgcacggc aagcaggccc tgcacggctg
      901 gcagatcaga gaggccaacg cgcgtcggaa tctgcggcgg cgcgtctttg tcgacggcgg
      961 gcgcggcgta cggatcgacc ggcttgccgg gatccgtctt cgcgcccttc gggtccgcct
     1021 tggcatcctt cttcgcgtcc ttctttgcat ccttcttcgc gtcaggcggc gggacatcgg
     1081 ccttcgcgtc cttcttgccg acgtcgccgt acggatcctg cggcgcagca tcggcgtacg
     1141 gatcgaccgg gccttccttc ggcgcgtctg ctttcggcgc tttcttcttc ggcgcggctt
     1201 tcttcgcggg cgcaggcgcc ggtgcggtcg gcaccgctgg caccgacgtc accggctgcg
     1261 cgggttgcgc ggcggctaca ccgacaaacg acaacacgat cgcgatcgcg acgaggttct
     1321 tcatttgcgc acctttgcca gctcggtgat cagctccgaa tgaatagcac cgttcgacgc
     1381 gactgcttcc ccggagtgga cgtcgaaccg agcgcccagg gtgttcgtca cggtcccgcc
     1441 tgcctcgacg acgattagcg cgcctgcggc catatcccac gcttgcagat cacgctccca
     1501 atagccatcg aaccagccgc gcgcgaccag gcaaagatcg agcgctgcag cgccgagccg
     1561 ccggcaggtt gcggcacgcg agaagtgctc ccattcggcg taattctcgg cggtcgtggc
     1621 gatgtacggg aagccggttg ccagcatcgc cttgtcgagg gaggggcact cgcttacgtg
     1681 aagcacggta cccgcgccat cgcgcgcgcc gccgccgcgc gtcgcctcga accaccagtc
     1741 gagcagcggc gcatagacga cgccgacggt gagctcgccg gcctgctcga gcgcgatcga
     1801 gacgcaccac agcggcaggc cgtgcgcgaa gtttacggtg ccgtcgatcg gatcgacgat
     1861 ccagtgcgta tcgccgcgcc gcgtgccgtc cttctcctcg cccatcaccg cgatgccggt
     1921 cggctcgagg atcgcgcgaa tcgtttcctc gctgcgcacg tcccactccg tgacgaggtc
     1981 cttcgcggtc gacttgccgc ggatgtgctc cggccgtgcc gtgcgcaaga gctcgccagc
     2041 tgcgcgtgca gcacgcgaac cgatggccag cagctcgccg agatcgctca cagatcgagc
     2101 cgcatcgcga cgagccccgc acccttcggc gcggtgaacg tctcgctggc ggcgacttgc
     2161 ttggacagcg tcgaacgatc gacgacgcgg aagccgaact tcgccgcgaa gaagtcgctc
     2221 gcggattcgg tgagcagata gatgcggcgc acgccgcgcc aacgcgcctg gctgaccgcc
     2281 atgtcggcga gcatccagcc gtagccggcg ccgcgacatt cctcgtcgac ggcaagcgcg
     2341 cgcagcacgg cgtcgtcgcc catcacgtcg agcgcgaccg agccgaccat gccttgctcg
     2401 ttcgtgagca cgtagaacga cgggaactgc tcgtcgcgcg cgggctcctc ggcgagaccg
     2461 acgcggccga gcaacgcgcg aacggcggcc atgtcgccaa ggtgcgcggg cttgagcgga
     2521 tcgccgattt cgtcgagcag cttgtcgtgc aggccgtcga agctgccgct cgacagcacg
     2581 cacacgacat cgccgggcgc cgcgctctcg gcaagctgct tgacgattgc gccgacctcg
     2641 ggcgtgtagc tgcccttcgt gccgctgcgg tgcagatcga gcgcgagctt ctcgggatcg
     2701 aaacgatcct cgacgggaat cttgttggga tcgaacagcc ggccgacgat cacttcgtcg
     2761 gcgtgcgaga acgcgtcggc gaactcgtgc tggaacgtct tgcggcggct cgtcgcggag
     2821 cgcggctcgt agacggcgat cagccgacgc ttcgggaagc gcctgcgcag cgccttgagc
     2881 gtctcgcgca cggccgtcgg gtgatgcgcg taatcgtcga gcacggtgac gccgccggcg
     2941 ataccacgca gctcctggcg acggcgcacg ccggcgaagc tcgagacacc gcgctgaatg
     3001 gcgctgatcg gcacgccgcg cgaatgcgca accgcgatcg cggcgacgca gttcatcacg
     3061 ttgtggcggc cgacgagcaa cgactcgaag tgctcgtagc gctcgccgtt gtgatagacg
     3121 tcgaacgcga cgcggccgga cttctggatc tcgaggttgc gtgcccacca cgcgacgtcg
     3181 gacgcgatgc cgctctcggt gtcgacgtcg aactcggcgc cgctttcacc gccgccgagc
     3241 accgcgtagc cctcgacctt gcacgcggcg tacttcgcga ccgcgcgcgc atctggcgac
     3301 tcggcgcaga cgacgagcag gccgtcctgc ggaatcagcg cgatcagttt cttgaacgtg
     3361 tcgcgcaccg catcgaacga cggaaagatg tcgacgtgat cgagctcgac gctcgtcagc
     3421 acggcggtgc gcgggcggtc gtgcgcgacc ttggggccct tgtcgaagaa cgcggtgtcg
     3481 tactcgtcgc cttcgatcac gaactcggga ccctggccga gccgccagcc ctggccgagc
     3541 gagaccggca cgccgccgac gaacagcgac ggatcgcgac cggcttcttg caggatgtgc
     3601 gcgatcagcg acgtcgtcgt ggtcttgccg tgagtgcccg agacgacgat cgagtgcttc
     3661 gcgtcgagca ggcgctcgcc gagcaccgcg gggaacgacg tcagcttgat gccgcgctcc
     3721 tgagccgcgc gaacctcgat gtgatccttg ccgtgcacgt tgccgaccac cacgagctcc
     3781 ggtccccaat cgagattctc gggcgcgtag ggcatcatca ccgggacgcc gagcgccttg
     3841 agctgctcgc tcatcggtgg gtagacggcc gcgtcggatc cacggacgtc gtgacctgcg
     3901 gccgcaagca gccctgccac ggcgcccatt cccgtgccgc cgatgccgat cagatgcacc
     3961 ttcacgattc ggtgacggac cctaccgggg tcgcctcgtc gggtaaagtc cgttgtgctc
     4021 aaagaatacg ctatgatctc ctaggcgaat ggagggccag cgccacgagc gacgccagta
     4081 ctttcgcggc aaggctcgcc ccgaccgcgt attacaggtc cgctaccgca cgacgcttca
     4141 tccaagctgg atcgcggcgg agacccgtaa catcggtgta ggcggcgcat tcatcgcgtc
     4201 agctgacgta cagccggttg ggacggcgat gaccctggag ctgtcgttac cgacgagcga
     4261 tcaggtgttc acattgcccg ccatcgtgcg ctgggcgcgt gaccaggagg gcatgggcgt
     4321 gcagtttgtc ggcgtcgacg tcgacatcct gctcgagctc aacgactact tctcgtcgct
     4381 gactacctga tgacgtcggt cgactcgaag atcttgtccg ccgaggccgg cacgaagccg
     4441 tggaacaaat cgccgctcgg ggtcgggtag cggcgggcga actcgtagta gcagctgggg
     4501 acgcgcgtga tgtcgccggc gaggtcgacc tcgatcgagt cagcacgcgt cgaggattgc
     4561 tcgagccgct gcgccggcgt gcctttgatg acgccgccgg attcgttcag cgtgaagccg
     4621 tgatcgatca ggaatgcgtt gagcgactgc agatcgggga acgtggtcag cgcgttgacg
     4681 agcaccgtga agtgattgac gcggaacccg aacgccccga cccacgctgc gtactcgctc
     4741 tcgatgagca gcgactcgta ctccgcacgc cgcacgcgcc acggccgtcc cgcccacggg
     4801 aggtccgcgc gcgctccgaa cccggcgggc agctgcgcga cgaggccgtc gatgatctga
     4861 taggccgccg cggatagctc gctgacgcac agctcgctga tgaacacttt cgggagcgca
     4921 ggatccggat gctgccagta gcgcgcgacg agcttcttgt cgtcgaagcg atagcggtcc
     4981 ggctgcatgc gccagccgag cgcctcgaac ggctttgcga gcgcatcgat gccgatgcct
     5041 ggaaggccgt acgtccgcag cgcgacgtga tcgttctgca cgcgttcgcc acgcgcggcc
     5101 agcaggcgat ggatacgttc agcttgcggc gtcgttgcga cgtagtcgcg ccacagcgcg
     5161 gtcagcagct cctcgacggt cactggagca tcagcgggaa gttgatcgac gtcttcgcct
     5221 tcttgctggc cgggaacgtc gccttcttga ccgcctcgtc gatgcactga ccggtcggcg
     5281 tgttcacgaa gtcgccctgc tggtcgtact tgaggaccga cccgtcaccg gcgacctgga
     5341 gcttgagctt cgcgctgccg ctgacggcga acttcttgaa gcagtcctgc gcggcctcgg
     5401 tgaccggctt catcgcttcg ctgatcagct tcggcgtgag ctggtcgagc tttggttcag
     5461 ccgaagcgga cgccgccgcg acgaccggac cgcactgctt cttgtccgct tctgccggac
     5521 ccgactgcgg gttcgcgagc acgatgctgc catcgcatgg cgcgaacacc cggtagccaa
     5581 cgaggtcgag ggcaacgacc tgcttgccat cgatcgtggt cagcggcttc gccggaactt
     5641 tgacgagcag ctgcacgcgc gcattgctca ccgagcgcgc gaacgtcttc gccgccgcct
     5701 cgtcggcaac ctgcttcgcg gagtcgccaa tcggcgcggt cttgaacttg ccgccggcga
     5761 acttcggcga cgtcaggtcc ttgtcggtga cgacgtagaa cgtcttgccg tcgatctcga
     5821 cgccggcgca ccggatgcaa cccgacagcg tcagcggcag cgacttcttc tgcgcgctcc
     5881 acgcgccagc gtcgaacgca tctttgtcgg cgtcgatcat caacgtcgag ccggcgagct
     5941 gcgccgcacg cgtgttgcgg accgtgcggc actgacgctg ctgcgtgtcg tcgccggcgt
     6001 cgcacggcgc ggtgaatgcc cacacgaggc tctcgacgcg gctgatgcgg acggcgttct
     6061 gtgccttcgc ttcgaacgtg tcgtcggcat gcgcaggcgc aggcgaagcg gcgatgagga
     6121 acggaacgat gatgagtgcg cgcattactg gagcttgtgc gtgaggtacg ccttgagacg
     6181 gagctcggcg tcggtggggt cattgatcac gatcggcacg gtcttctcga gctcttcctt
     6241 gagcgccgtg tagagcgact tgcgcgccga gtcttcggtg agcagctgcg cggtcgtgat
     6301 gccgcgcacc ggggtctcgc cgaccttcgc gcagtacgcc ttcagcttgt cgtagagcgg
     6361 accgccgagg aacgcggaga tcttctcgga ctggtttgcc atgcgctcga gcgatgcttc
     6421 gacggcggtc ggcaggtcac cgcgcgcatg atcctgcgtg agcgccgcgc acacgcgcag
     6481 caccatcacg tcgtcgatca cgccgatgcc ctcgttccag tccggaatca agtccatctt
     6541 gctaacgaga tactgcaggg caccagccgc gagcttgcgc gacttgtcgt cggctttcgt
     6601 cgactcgagc aacgccttga acgcgtctgt atcctggcga atcgtctcgg cccagctctt
     6661 gaagacgtcg atatgtccca cgcagtcctc ctgtggcccg gaccgtacct caattgtgta
     6721 ggatggatct atgcgcggcc tggcg
//
LOCUS       DASUFX010000557        31020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_367019,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000557 DASUFX010000000
VERSION     DASUFX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 31020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31020
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367019"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFV99_22285"
     CDS             complement(<1..360)
                     /locus_tag="VFV99_22285"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062122.1"
                     /translation="MHDLVGRDRELTTLDEAWRRAMAGDRTVALITGEPGIGKTRLLD
                     ELAARVPASGGSLAWGRTAEIGHTPAFWPWLQILSALEVDDDRAPAIGSLEQHPGAAS
                     RLALFDATAAFLARRAAT"
     gene            complement(381..896)
                     /locus_tag="VFV99_22290"
     CDS             complement(381..896)
                     /locus_tag="VFV99_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062123.1"
                     /translation="MKTLTKLIPVFALMIPAAATADALPKQVEAMQCLVGTWKATGTV
                     AMGKDTAKVTATWTCKATSAKFGVLCALDLAGIPGVPQYAETDLFGYEPNSNTAHWFS
                     VTNAGETHDHVAPMPDGNSAEFVYTGTQEGKPFKEVIKLTFIDKKNITVRAETFVAGA
                     STSVFDIKAKK"
     gene            complement(1064..2824)
                     /locus_tag="VFV99_22295"
     CDS             complement(1064..2824)
                     /locus_tag="VFV99_22295"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5062124.1"
                     /translation="MRGLVVVLFVAACGGGSTPVGHDCTDGDTRSCYRGQPGTEGVGP
                     CKAGLETCAGGVWPGVCVGDVTPFVETCNGKDDDCDGQLDNAEDSGDSCTIGGCPGVR
                     GCASDGDVACIAFPENECGLCGGPPVSGLGSDCTNGVCSGSLVCSTDKMSAVCSAPSL
                     NECEVCGGPAVPNLGTVCTATAGCAGYLACNAGGTGSFCDCGANICNDGGTIRSIVGP
                     ETGDLVITEVMPSPSKVSDTVGEWFEVQVMRDVDLNNLGLDRAGDTANPNVIRAFDCM
                     HVTAGTTLVFAKNPDPVMNGGIATASIYGTFTFSLVAGTVTSPGDVQILAGSTVVDAI
                     TWTSSRSGKAIQLDPDFTDPTSNDNTSNFCDATATYGLGDFGTPGTANGQCVALPPPG
                     MCDDAGTLRAIVKPTANALVISEIMPNPKTETTQEWFEITNVGSTAFDLNGLGLDRNG
                     DSALPNVVNSAACKPLAPGAYALLAHSADPTTNGGLPTVDATFSFSMVNGNGDVQVVD
                     PTTCTGTPVTCTTVYDALAYTTANGWPATATADGVSAQLEPTNLTTTDNDTFASFCAA
                     NTDYGTAMNKGTPRATNVCP"
     gene            complement(2938..4119)
                     /locus_tag="VFV99_22300"
     CDS             complement(2938..4119)
                     /locus_tag="VFV99_22300"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062125.1"
                     /translation="MRSLLVAMLVVSACGGRTAKTTLPTQSPPVAPARDLADARSAVP
                     TIPTDAHLVAKDPRIVDLDIIRITANSRGVGGEPEMDHVATADLFKQATEAAKGGEPE
                     RAINIYRRLVTEFPDSQYAPVALFNIAAIYDARGDLDTTIATLRELVKAYPNARESID
                     GHLYIAALQTDHDKFSDALVTLDEVLPRPNLSYADRIEAFARKGYVLIELHRYDDAEA
                     ALGSAVGEWKKVTKLDDPYYIAMAHYYRGELAHRKFTEAPARLPDDVLVADLEKKRVL
                     AVAAYDRWKEALGFKQAYWATASGYQMSQIFVELWEAHVKAPYPKRIDAATRQTYVVE
                     VHERVKEHLDKALEGHRMNVELAKAYGVETVWSKASAKKAAEVMELLAREGAGQYVTP
                     D"
     gene            4227..6641
                     /locus_tag="VFV99_22305"
     CDS             4227..6641
                     /locus_tag="VFV99_22305"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5062126.1"
                     /translation="MKVAFTYNLRLTDVRETEKEAEYDSADTVNAIAAALEAAGHEVE
                     KVEVSGPATSLLERLEAIDPDIIFNTAEGDRGRMREAFYPALFEELGIPFTGSDAYTN
                     ALTLDKWVTKLMVQRAGIDTPRGVLVTVRNYESVLERGAGLAFPVIIKPNHEGSSKGI
                     YNGALGSSVIKEPKDLNGALKTALRAYPDGVLVEEYIDGLDVAVGFIDGVGHDDGLLT
                     PVEMSYDAPGENERFKIYDYRLKNVDPTKVQYRCPANLPRDVAARLRQISHEVVRTCG
                     LRDMARLDFRVNAEGRIYLLEVNALPALATSSSLFAATAQVGLTYQATIQSILNAAAL
                     RSGLATASQLGVTKQRKAQPIRVGFTYNVKRSADGDDEAEWDPPETIIAIANALARQG
                     HIVVHLEATPDLPRVLAEADVDLIFNIAEGVEGRNREAQVPALCELLGIPYTGSDSAT
                     LAIALDKALGKKVLLQHDILTPKFQLMESARERISADMKYPLIVKPNAEGSSKGIGST
                     SIVDDEEELRAAVKDCVERYRQPALVEEYIAGREFTVGLLGDKRPRVLPPMEIKFKKN
                     DPRPVYDYTVKQDWEEYVYYECPAKLTEAEQKAMEKIARATFWALDCRDVARVDMRMD
                     AEGRIYVLEVNPLPGLTPDYSDLVLIAKASGMEYDQLIAEIMQGGLRRLREKKREERE
                     EREAREREAAKNGDKDKAKRVPKLDKADSREGNGRAEARENGQREGSGNGNGNGNGNG
                     NGNGNGNGKSDGTVRAVKRERSEPTAAAANRERDRDSERNERAKTDPPDRDPSDLSPA
                     AGTSTN"
     gene            6864..7412
                     /locus_tag="VFV99_22310"
     CDS             6864..7412
                     /locus_tag="VFV99_22310"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5062127.1"
                     /translation="MDISSLDPSSDADRERCAALLVAEFRDIAPDAWPTLATARESVD
                     ECLALGVVRVARENHDIVGWIGGHHTYGRVWELHPLVVDRAHQRRGIGRALVADLERI
                     VAGRGGLTLELGSDDEVGLTSLAGVDLYPNPLDHLMRLEDRGGHAFVFYARCGFAVTG
                     VTPDANALGQHDIHMAKRVGER"
     gene            7694..7945
                     /locus_tag="VFV99_22315"
     CDS             7694..7945
                     /locus_tag="VFV99_22315"
                     /inference="COORDINATES: protein motif:HMM:NF033706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO4226 family nickel-binding protein"
                     /protein_id="HEX5062128.1"
                     /translation="MPRFIDIHTGMKGVTKQQLSMEHEKDIEAQKSEGVRFLHAWADP
                     QSGKVFCLSEGPSKDAVLRVHKKAGHATDEIYEVPIEVE"
     gene            complement(8086..10854)
                     /locus_tag="VFV99_22320"
     CDS             complement(8086..10854)
                     /locus_tag="VFV99_22320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062129.1"
                     /translation="MSDAEPTANAHSYEVLGKLAEGETTEIFLARAKGAGAVERFVVL
                     KRLKRAVTSHVERVQAFLAEARLASQLQHPNVAQVFEVGKLGGSYFFAMEFVNGETLQ
                     SLMHHARSKKIQIPVRAVLTIASAMAAGLHHAHERRGTDGQPLGIVHGDVSPTNSLVS
                     QEGIVKLVDFGIAKAGERVPGKVSPYASPEQCRGEPLDRRSDLFALGVVLWEVLTLDP
                     LYQRTGGDDEIKDAIETEEPPKPSAKRYDVPPELDAMVLKLLAKDPADRFQDADELLA
                     TIEALAQKLSILLSTADLSRMMRLWFGTKSDPTVDPSAPDAVQPLLVASEQIPDDVTA
                     PSHTAVDDQLDAIRSAATLITARATRAGSSTGRQDKPTVPPELMDNPNENFEQIRDRI
                     LARARAKKETRNPPLAAQAAAGAPGAEQGHLTSGAVAASATPPADDRHTRSTALPPLG
                     APDRHTRTTAQAPIAVIEEAVRLTGVADPVVKPNGTNGHHPVAAAAELAKTEPAKPAE
                     VARVALVKAPAQQSVPEPVKDNEPDKVVVDEELSGSRSASGELGNKGTSGLIVLAQAK
                     VSVDDQPAQSREAPPATAKLTDDKAADKDDDIVTRKRNEAARLAEAAARTREAFAREA
                     AARDAAHARNSEDRAESEGEHTETEAERTERVHHARASTAEIALPPAPRRSGWIVPVL
                     AFACVAAVFLVVMKLRGGSKPTSANAQTPKTAVAPPTNPSPHATPQVAPPAIDAAVAA
                     VAPVDAQAVVVATPDAAAQVAQNGPGDGAANDPGEKKDPGEGTKKDPGQKADPGEQAA
                     KKEPVHKKEPAEKKEPAEKKEPVEKKQPEPAKGIEELFAADDFAKTNTACTKEVVFNA
                     EKLQMCALAACQTKNTALAKRWINAIAKASRPELIEKCKSLGVDLEPAPPSAPPAAPA
                     PAPAPATP"
     gene            10967..12427
                     /locus_tag="VFV99_22325"
     CDS             10967..12427
                     /locus_tag="VFV99_22325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5062130.1"
                     /translation="MKVATRITVATAVVVALASAAYAYFDLRARKAERVSSLAGEAKS
                     MAAVLRPQLEQQVSAFRYPSDEQYKQWLKTTGWTIDVVPVRATTPADMSRQAGWLRVM
                     VDQPSRTFSEVVGDRYYYAISLVQRVARDADSEPQPVTSAMLELSKSTDVLTGDDVGR
                     AITLIVLIVVVTTLMVGALASRFVSRPITKLLTGIDDVAKGDLSHVILAERDDEIGQI
                     ATRFNEMTFSLRESRGETQRQNEAKLALEQRLGQTEKLATIGQLAAEIAHEVGTPLNV
                     IAGRARSMQRKSKDPEVVEKNAEIVAEQTARITRIIQRLLDFTRRKVGATGKAEINLN
                     ELSLTTMELLSGQFSAAKVRTRLERAEGLPRVAGDADRLQQVMINLMLNAVQAMPDGG
                     SLAVETKSVRRTRPGLEGSAEQPFVAFSVKDTGIGVPEEIRDKIFDPFYTTKDGQGGT
                     GLGLAVCSGIVKEHDGWIDVEDNDGGGTIFRVYLPA"
     gene            12461..13321
                     /locus_tag="VFV99_22330"
     CDS             12461..13321
                     /locus_tag="VFV99_22330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5062131.1"
                     /translation="MQRVHIEYMGDSVELPLGETVVGRDVACALRFNDPSVSRRHLRF
                     IRRSNEVFVEDLGSSNGTLLNGRAVAAPLRIVDGDTINAGSRVLTVRISDGDDEQPST
                     LVLKTFNAASEIKQAASRATLRNLPTVKPAPQAAHQRCPRCAAPVSELDEECANCHYH
                     WGSFRPMSRTDVRPNPIERRRHERQVVELHLVYTSSELEIEALSRDLSESGVFVCSQV
                     LDPIGTECELKILPDGCPPIDVRGVVRRVVEREIAGDPIGLGVEFVGVGEPQLTWIRA
                     TIEKLDAGVS"
     gene            complement(13324..15669)
                     /locus_tag="VFV99_22335"
     CDS             complement(13324..15669)
                     /locus_tag="VFV99_22335"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5062132.1"
                     /translation="MTTLTRWKLTCALFAALAGVATVHAYRGSAAHNAPAKLASTPRG
                     TLPIQLRKPIHVSPDVIGVSQRDLVDRIFAAKSVRDIQLLADKLGMVGDDDAIETLSP
                     LLEDPRRGVPEAMLAAFGQIGTQKAVDVLVAHVKDDRPTIRYAAISNLGATHSEQAEK
                     VLLELSRQNGDPAQNAAVSALGTLGTDNSVARLIELANLPDYSVASSAVYALGIVGTQ
                     ASELALRKLVDAPDSRVAAAALASIDTIDKPMLEKLKTIVKAGDPQLVSAALSALGHA
                     GEDGLPVLREAALYGGMNARWAAVNAIGEVGGDKAVQTLGEILKIGDRQAASSAANAL
                     ANLGGQEARELLIDAALSDRASMTGALAQLAQMQGDDVEQALLSVVKQGSSAERRQVL
                     PRLVKEGNPEAITMTIELAQKGSRNEKYEAMRILGDAGTPKAFDALLDIASKARGQTR
                     VQALDMLAQSHPADPAVGQLLSDALFSGRRDEASYAASVMGRLGTEDSRQALITALTG
                     SDKELQAVAAGALGQMGMTENVKAALLGAARNNPQVKMQVMQQLVQNGQPEGLKLAEE
                     ILNGKDAGNASQVVWALASNGTNEAKRLIEKAIQSKDPSVRVAAISSLSQNPDERSTD
                     VLMNLMRDQDPQVRSMALSTLGQIGGERAQQAVLNATRSGTPDERIAAISGLAQMDDA
                     NASRQLANLMRDSDVNVARAAISSSYNGGPEVDTTLTQIVNDPGSSEELKAAAASQLR
                     VRGTDLDDRTEQIVTKLAGASGGYGGYGYGGYRYYPGEMME"
     gene            16076..16450
                     /locus_tag="VFV99_22340"
     CDS             16076..16450
                     /locus_tag="VFV99_22340"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5062133.1"
                     /translation="MLTEEQTNELRARLQADLTRLADHAHAGLEFTMDRDRDRVGRDS
                     MDESTEEELYSTQLRLHDRETFLLNKIHEAMRRLDAGEIDECEECSEPIGFKRLMARP
                     VTTLCFECKTAREQVEADERPE"
     gene            16558..17898
                     /locus_tag="VFV99_22345"
     CDS             16558..17898
                     /locus_tag="VFV99_22345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062134.1"
                     /translation="MRDRYTITERLDHGGMAEVFRGVAESMEGFKKNVAIKRILPNLT
                     KNQKFVSMFLDEARLSLFLQHANIVQVFDISRTPDNAYFLVMEYVDGCNLKSLIERQK
                     QKGRRIEISHTIYIMMEACKALNYAHMLEHPETNEPLGIVHRDISPPNILLSKNGEIK
                     LVDFGLAKANTQIESTDQGVVKGKFSYLSPEAASGLDVDHRADIFAIGIILWELFTGR
                     RLFYGDTDYQTVELVRQARVPSIAALNPEIEPELEAIVRKALAKDQADRYQNAADLGD
                     ALAQYLFSRRMKVTARDVSGLVKDTQVEMMRKRSTEPKESLIDALILDEMQKMTSLVD
                     GEKDAGDSGSEGSMSLDPMKFVDTSNWASEIAHSGGQPKRSGRASGAPGNKPRVTSPP
                     TPSPGDMESLEAMLEPERTGVHGKADKKTPWVLIIIVMLVLAAGAAAAVVMMMT"
     gene            18167..18706
                     /locus_tag="VFV99_22350"
     CDS             18167..18706
                     /locus_tag="VFV99_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062135.1"
                     /translation="MSGTQYSEKFRARMVEKMAGPNPMSGVQLAAETGVPQPTLSRWL
                     REATTLRRKMAKRQDDEEQKLVAADAPQRTAEEKFSLVLEAAAVPEAELGEWLRRKGV
                     HAAQLEEWRAQAMAGLTGTEKRSKESATDAKHIRQLERELARKDKALAEAAALLVLQK
                     KVQAIWGDADDDTDEKKDK"
     gene            18703..19773
                     /locus_tag="VFV99_22355"
     CDS             18703..19773
                     /locus_tag="VFV99_22355"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX5062136.1"
                     /translation="MIRTLVDEAVAAGARREAACKVVGLRARTLQRWEHTSDDNRHGP
                     KHAPANKLSDAERRKVVMVATSAEFRDQSPKQIVPTLADRGEYLASESTFYRVLHENG
                     MQQHRGRSRPPMPRPRGHAAYAPWQLAAWDITYLRSHVRGQFFYLYLVEDVWSRKILG
                     WDVHDVESTDFAADLVERIRAEANAEGVDLRGWVLHSDNGGPMKGATMLATMHKLGIV
                     PSFSRPRVSDDNAYAEALFRTLKYCPEYPAKGFATVEAARAWVARFVAWYNGEHLHSS
                     IGFVTPSARHAGDDVALLAARRRTYDRARRQHPTRWSRHARAWARPEIVTLVPEDLIS
                     LTRSDQIAENRPAALRLTRQQP"
     gene            complement(19864..20988)
                     /locus_tag="VFV99_22360"
     CDS             complement(19864..20988)
                     /locus_tag="VFV99_22360"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX5062137.1"
                     /translation="MQVCRWLAGAAALTACDPTAIAPDLRVGTPCPAVVMRELNNAFI
                     ATHPAPVRGMSWQHADYFMGDLAAAETVGEPIYVDYAFRFGDINKWELIGETDTRNPD
                     NQAAGQVYFELYEIVPDPVRTAELAKSLDWVIRDVRRDSWQNTRALFHAAPAWAQIGS
                     LTSDQRYFETAFQLFVFARDVIQLFDPDAGLWYTDLEHRFPQHKTPNDKKIFWSRGNG
                     WVFASFARELPFVPADSRFRPDYEATFRTMAAAIAPLQRDDGFWNVSLFDPEDHPGPE
                     SSGTSLFTFGFAWGINNGLLDRDTYLPVVERAWLGLNTIAVRADRQLGYVQGLGGGLA
                     DGPDDAQPVSILSTEDYGLGYFLLAGNEVAKLGLDLGCTP"
     gene            complement(21013..24738)
                     /locus_tag="VFV99_22365"
     CDS             complement(21013..24738)
                     /locus_tag="VFV99_22365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062138.1"
                     /translation="MLGQALLALKRYDEVLAEMRVALELDHSSIQSQILKAEALLRKG
                     DVAPAIEVLHKARQAAPGDPRVLQLLGEAENGSAHRPSVTHPSVGFIGAGDTKHYPGH
                     SSGDDAPEPEGLYTRPTSMQSPGGARRSSKKRAVGAPDPTPPPGVFDAGDRTGTVEVD
                     PELEGVEVEDDIDFDDLAAPPKAAKPGKIGGERGAVVSSRVNPARPTAKAGDGEKGTA
                     IGKKSAKQGFDFDDDADEDLGETRSPFDKPGARVPGPRSEVRNAVAMPSGVIGGPPAP
                     FASQRPKGKTVPPPRSKPASAQPPMTMTARGVGSMPPAAPLPPAPQPPIAAALPTMAA
                     APGPSPFSVPAVSANRPTMAVAPPPPPPLHQENLFSEGMPAPSWASATVGPAMDPRAL
                     DPRAIAAANEPTARPSELDPRILALMSGQPEPNPAMALDQMSVVGPGKAGPKTGMRKA
                     RSRAQVAIWVVVGVVLIGGGVFAGIQIRAMRLAKQIVAARQRATDLAKDDTWKGWRDA
                     RESLADIVGASATLGNRAALARTRALVAYEFGDGIVDAKADVDALAGQGGLDGTLATA
                     YLALAMDDAKAAKAAADAALSASADDAAAHYVAGQAALLAGDPKTAITQLKAATDKEM
                     RPLYGVGLARAYAAAYSWDEALATLDKVFAITADHPAAVIARGDILAASGRIFPGSAM
                     ANEVRAQLDRVVTQGKRPLTEQQHGVSPAQVAFGHVALALINYMRNDQKAARNDLKTA
                     AAINLDDVRFAEDFTDALYAVGDLSIARNAADKSLKDYPASKRSRIALARILLAQGKA
                     ADAVEIISKQADVMMLPDALAVRGQAQLAAGDPASAAADFDAALKKVPNHEPASIGRT
                     WVELSTGEVDAATKHAAERYNPKGSSPALTTVYAATLRRSADPAAREKAKDLLEKLVQ
                     TATGFDLARAQLELARIYRDLGDYRSARPAYAAAAASGSQDARLEYALLLIEDRDPNG
                     GRETLDSLLHDAGERPTPQLVIETARARMLVGDHVDAQQLLDSADKLSGVERWKLDRE
                     RGRLALRKSDFPVAASALTRALDGCGSDGETFLLAADVGIYEKPLADKVKKLAPERLK
                     NKPEAKIVEGKQLIAAEKLAEAEQAYKDAKAGLKAEKASPRRVAQADFGLGVVAYIQQ
                     NNAVAQQFFDLVIDQDPSLVDTYVFAADIAKDPRKSFQFAQRAVQYNPDYPGAWLLVG
                     KLASRLGERKVLTDAINRLTQIAPNGEELKDLKALRR"
     gene            complement(24926..25390)
                     /locus_tag="VFV99_22370"
     CDS             complement(24926..25390)
                     /locus_tag="VFV99_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062139.1"
                     /translation="MRAIWFVMVLAALAGCASAKTGDTLADSVRQYNDGVRWERFPVA
                     AVHVPPKERATFIEQADDRAHDLKITEYDVLRIDSKSDREATVQIKMSWYRESEGLLR
                     ETQSLQSWEKHGKTWWMVDEARFKGQEMPGLEEPVGSPVGLGAQEPLSKTQE"
     gene            complement(25390..26985)
                     /locus_tag="VFV99_22375"
     CDS             complement(25390..26985)
                     /locus_tag="VFV99_22375"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5062140.1"
                     /translation="MSNEPKATGAGRGVLYIAFAKFYFMFVGLVVQVRLPAILSHAAW
                     GSYSFINSLVSPVNNVLVTGTIQAVSKFAAAEPEKARKVQLAGLRMHVRLGLVVAILF
                     IAAAPLVSWLFLDMSKTAPLMLAGLIVGGNAFYAVFVGTANGQRQFQKQAGLDITFAT
                     MRSIGLIGMAMAGLGVIGVVGGWVAAVGAILMIAMTWIGLPGKAAHDGETIPVKPMIN
                     FFIGVAIYLALFNALMFVDTWLLKRLTTEYFATHKAELAAAVDQVVPWAPKVSGYHAT
                     PSILADVQVGYYAAVQNLARLSYQAIIAATFVVFPLVSHSTFADDRETTQRYIKVTTR
                     YSLMFAMAIAVVMAANPVDILGLVYAPDYAQQGGAALAVLALGNVAFSVFAIAGTILN
                     GAGMSRAAISTAALTLGIAAAGNFIVIPMVVESGRVLEAAAAVTGGSMIVGAVIGGWI
                     MRNRLGAFLPMISLVRIGIATAAALVVGRFLPLHGKLMTLVEAAVVGLTFIVMLVATR
                     ELGKRDLDAIRAVRTKRAPGGGE"
     gene            27217..29175
                     /locus_tag="VFV99_22380"
     CDS             27217..29175
                     /locus_tag="VFV99_22380"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062141.1"
                     /translation="MRGFSIATKCANVAELIEKFRDRVDEESILVTTVESRDIGTECA
                     FAILLADKKVGLAGTCVVRDVFTDPNNPFKRPGMRLQIKRLGPESQKVWTELVAKRIS
                     PRKMTQALPVIAMPSMPGKKPRVATIPPVGDRPSSPALTAKPPVIAPPVAARPVVAPP
                     VAAAKPVVVAPPVVAKPPETAPPVAAKSPEVPPVVAKPPEAAPPVVAKSPEAPPVVAP
                     KPVIVMPRAKGRNTGEMLDVVADAPTRRPPTPTPQRVRDDSQEPLARAPRATPFDLDN
                     LAKHSPAVFDAKQPDLTTPDAKNLDEQEAERKAEAKKLEAKQAAAKPVEGTRTPGSPY
                     VLPANPLANIADSSLEGYVDVRLTEAFGGDAPAAFPQGTLDDPPVEPEPPMVLAPPPP
                     IDEIAVILARPPVDEVPVTGSGRARPASVTAPLKPMITAKPPTRTQAPTKRDAPVAGV
                     RERHSEPLPLPPPPALPPMAATTPLPYAATKLPNLNVPDANDRVRGPLLKRRVRNQLL
                     LAVLLVPLLGAGAMVAYMQLATAPAATVTPLAPAATPPLDEPLTTSVPVEPAPPAPAI
                     ADPAPTLPTRPQPIHAVLVRTAPIAARVTVNGRSFGTTPTYVKIPANTPVELVIARPG
                     FKPVKYPFTSKTATDRVFVRLERARGGR"
     gene            29172..29642
                     /locus_tag="VFV99_22385"
     CDS             29172..29642
                     /locus_tag="VFV99_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062142.1"
                     /translation="MTPSVRGFVVATKCETIEELVEKYAPRSDDQSIFVGIVEERVLG
                     AECAFAILLADKQPAFAGICQVLEVFRDDNNPYQRRGMRLGIRKLGITSERLFADMAA
                     MRANKRRFARGSTAPPEADLDGVPIVVDDADDEMSDSIPIDLEDELSGPARARS"
     gene            29725..>31020
                     /locus_tag="VFV99_22390"
     CDS             29725..>31020
                     /locus_tag="VFV99_22390"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5062143.1"
                     /translation="MQRILRGLWLPICLASAACGTETDEQLNETEQEVIVGSLAARFD
                     DTVRGGGVVAQGASLAGREDAVANTTAAITIAGIPGGSTVQRALLYWSINGGIDTTAT
                     INAVNVNGVQLGTGGSTCWGTNFVTFRADVTAQVTGNGVYTIGGLPSSTSATAADTNG
                     AALVVVYQNPTSGNRRRVMVRDGSMSTDATGDNVTDTFTGVAAPLASAGRFHLVVGDG
                     QSFPDGNLIFNGVTLGTDQFSGSDGSLFDTSSYNVNVPAALVNATWSEQTSSDCLGFS
                     TAVLDWNVATCGDAVRTGGEACDQGDTTNGDGCSSTCDVEAGWSCTATNPSVCTPNCG
                     DGIRAGAEQCDQGNTTNGDGCSSTCMIEPGYQCTGSPSVCTTVCGDGIIAGAEQCDQG
                     NTTNGDGCSSTCQAEPGWNCAGSPSVCTSVCGDGIVVGAE"
BASE COUNT         5112 a        10294 c        10401 g         5213 t
ORIGIN      
        1 ggtggcggcg cgccgcgcga ggaacgcggc ggtcgcgtcg aacagcgcga gccggctcgc
       61 tgcgccggga tgctgctcga gactgccgat agcgggtgcg cgatcatcgt cgacctccaa
      121 agcgctcagg atctggagcc acggccagaa cgcgggcgtg tgcccgatct ccgccgtgcg
      181 gccccatgcc aggctgccgc cactcgcagg cacgcgagcg gcgagctcgt cgaggaggcg
      241 ggtcttgccg atgccgggct cgccggtgat cagcgcgacc gtgcgatcgc cagccatcgc
      301 gcggcgccac gcttcgtcga gcgtggtcag ctcacggtcg cggccgacga ggtcatgcac
      361 cctcctctct tatcacgtcg ctacttcttg gctttgatgt cgaacacgct cgtcgacgca
      421 cccgcgacaa acgtttcggc gcgaaccgtg atgttcttct tatcgatgaa ggtcagcttg
      481 atgacctcct tgaacggctt gccttcctgc gtgccggtgt agacgaactc ggcgctgttg
      541 ccgtcgggca tcggcgccac gtgatcgtgg gtctcgcccg cgttcgtcac gctgaaccag
      601 tgcgcggtgt tcgagttcgg ctcgtagccg aacagatcgg tctccgcgta ctggggcacg
      661 cccgggatgc ccgcaagatc gagcgcgcac agcacgccga acttcgccga cgtcgccttg
      721 caggtccacg tcgcggtgac cttcgcggtg tccttcccca tcgcgaccgt gccggtcgcc
      781 ttccaggtgc cgaccaggca ctgcatcgct tcgacctgct tgggcagcgc gtcggccgtg
      841 gcggcggcgg ggatcatgag ggcgaaaacg gggatgagct tcgtgagggt cttcattgtc
      901 ttgtctcctt gccctcctgg cgttccatcg cgaggtacgc cgttccttcg tgaggaaatt
      961 cgacaatccg cgggcttatc cgcctgcgac aggctctgcg gatcgccccg ccggcaggta
     1021 cccgccgcca ctcgagccga gcgatccagt cccaggcgta gacctacggg cagacgttcg
     1081 tggcgcgcgg cgtgcctttg ttcatcgccg tgccgtagtc ggtgttcgcg gcgcagaagc
     1141 tcgcgaacgt gtcgttgtcc gttgtcgtga ggttggtggg ctcgagctgc gcggacacgc
     1201 catcggcggt cgccgtggcc ggccaaccgt ttgccgtcgt gtaggccagc gcgtcgtaga
     1261 cggtcgtgca cgtgaccggc gtgcccgtgc aggtcgtcgg gtcgacgacc tgcacgtcgc
     1321 cattaccgtt gaccatcgag aagctgaacg tcgcgtcaac ggtggggagg ccaccgttcg
     1381 tcgtcggatc tgcactgtgt gcgagcagcg cgtatgcgcc cggcgcgagc ggcttgcacg
     1441 cggccgagtt gacgacattg gggagcgccg agtcgccgtt gcgatcgagg ccgaggccat
     1501 tgaggtcgaa cgcggtgctg ccgacgttcg tgatctcgaa ccactcctgc gtcgtttccg
     1561 tcttcgggtt cggcatgatc tcggagatca cgagcgcgtt cgccgtgggc ttcacgatcg
     1621 cgcgcagcgt gccggcgtcg tcgcacatgc ccggcggcgg cagcgcgacg cactgtccgt
     1681 tggcggtacc gggcgtgccg aagtcgccaa gaccgtaggt cgcggtcgcg tcacagaagt
     1741 tggacgtgtt gtcgtttgat gtcgggtcgg tgaaatcggg atcgagctgg atcgccttgc
     1801 cgctgcgcga gctcgtccac gtgatcgcgt cgacgaccgt cgagcccgcg aggatctgta
     1861 cgtcgcctgg cgacgtcacc gtcccggcga ccagcgagaa cgtgaacgta ccgtagatcg
     1921 aggcggtcgc gatgccgccg ttcatcaccg ggtcggggtt cttcgcgaac acgagcgtgg
     1981 tgccggccgt cacgtgcatg cagtcgaacg cgcggatcac gttcgggttc gcggtgtcgc
     2041 cggcgcgatc gagcccgagg ttgttgagat cgacgtctcg catcacctgg acctcgaacc
     2101 actcgccgac cgtgtcggag accttcgagg ggctcggcat cacctcggtg atcacgaggt
     2161 cgccggtctc ggggccaacg atgctgcgaa tcgtgccgcc gtcgttgcag atgttggcgc
     2221 cgcaatcgca gaacgagccc gtgccgcccg cgttgcacgc gaggtagcca gcgcaccctg
     2281 cggtcgccgt gcacacggtg ccgaggttgg gcactgcggg accgccgcag acctcgcact
     2341 cgttgaggct cggcgcgctg cagaccgcag acatcttgtc ggtcgagcac acgagcgagc
     2401 cgctgcacac gccgttcgtg cagtcgctgc cgagcccgct cacgggcggg ccgccgcata
     2461 ggccgcactc gttctcggga aacgcgatgc acgcgacatc accgtcgctc gcgcagccac
     2521 gcacgccggg acagccgccg atggtgcagc tgtcaccgga atcctcggcg ttgtcgagct
     2581 ggccgtcgca gtcgtcgtcc ttgccgttgc aggtctcgac gaacggcgtg acgtcgccga
     2641 cgcacacgcc tggccacacg ccgccggcgc aggtctcgag gcccgccttg cacgggccga
     2701 cgccttcggt gcccggctgg ccgcgatagc acgaccgcgt gtcgccatcg gtgcagtcgt
     2761 ggccgaccgg cgtgctgccg ccgccgcagg ccgcgacaaa cagtacgacc acgagccccc
     2821 gcatcacctc atggtaaccg gggtcaggca ctactggtac tgcctgcggt ggtaaccggg
     2881 cttaaacgct gcttgcacaa cgttcacgac aacaagcagt accagtagtg cctgacccta
     2941 atccggtgtg acgtactgcc cggcgccctc gcgcgcgagc agctccatca cctcggccgc
     3001 cttctttgcg ctggctttgc tccacacggt ctcgacgcca taggccttcg cgagctcgac
     3061 attcatgcgg tggccttcga gcgccttgtc gaggtgctcc ttgacgcgct cgtgaacctc
     3121 gacgacgtac gtttggcggg tcgcggcgtc gatgcgcttg gggtacggcg ccttgacgtg
     3181 cgcctcccag agctcgacga agatctgcga catctgatag ccggatgcgg tcgcccagta
     3241 cgcctgcttg aagccgaggg cttccttcca gcggtcgtag gcggcgaccg cgagcacgcg
     3301 cttcttctcg aggtcggcga cgaggacgtc gtcggggagg cgggccggtg cctcggtgaa
     3361 cttgcggtgc gcgagctcgc cccgatagta gtgagccatc gcgatgtagt acgggtcgtc
     3421 gagcttggtg accttcttcc actcgccgac ggcagagccg agcgcagctt ctgcgtcgtc
     3481 gtagcgatgc agctcgatga ggacgtagcc cttgcgagcg aacgcctcga tgcggtcggc
     3541 gtaggagagg ttcggccgcg gtagcacctc gtccaacgtg acaagtgcgt cgctgaactt
     3601 gtcgtggtcg gtctgtagcg cggcgatgta gaggtggccg tcgatcgact cccgcgcgtt
     3661 cgggtacgcc ttcacgagct cgcgcagcgt cgcgatggtc gtatcgagat cgccacgggc
     3721 atcgtagatg gcagcgatgt tgaatagcgc gaccggcgcg tactgggagt ccgggaactc
     3781 ggtgacgagt cggcggtaga tgttgatcgc gcgttcgggc tcgccaccct tggccgcttc
     3841 ggtcgcctgc ttgaataaat cggcggttgc aacgtgatcc atctcgggct cgccgcccac
     3901 gccgcggctg tttgccgtga tgcgaatgat gtcgagatcg acaatgcgtg gatccttggc
     3961 gaccagatga gcgtcggtgg gaatggtcgg caccgcgctt cgggcatcgg cgagatctcg
     4021 ggctggagca accggtgggc tttgcgtcgg cagcgtcgtc ttcgccgtgc gtccgccgca
     4081 cgccgagaca acgagcatcg ccaccaacag ggatcgcata cccagttatg acgtatcaga
     4141 cgaccgaacg gttcgaccgg gatactcagt tggtttcact tgactgtttg ggtgtcacac
     4201 cgctttgcta agagtgcagt cgaaacatga aggtcgcgtt cacctacaac ttgcggctca
     4261 ccgacgtccg agagacggag aaagaagccg aatacgacag cgcagatacc gtgaacgcga
     4321 tcgctgccgc cctcgaagcg gcggggcatg aggtcgagaa agtcgaggta tcgggaccgg
     4381 cgacaagcct gctcgagcgc ctcgaagcga tcgatccgga catcatcttc aacacggccg
     4441 agggcgaccg cggccgcatg cgcgaggcgt tctatccggc actattcgaa gagctgggca
     4501 tcccgttcac cgggtcggac gcctacacca acgcgctcac gctcgataag tgggtgacca
     4561 agctgatggt ccagcgcgcc gggatcgaca cgccgcgcgg ggtcctcgtc acggtccgca
     4621 actacgagag cgtgctcgag cgcggtgccg gcctcgcgtt cccggtcatc atcaagccga
     4681 accacgaggg ctcgagcaag ggcatctaca acggtgcgct cggctcgtcg gtaatcaaag
     4741 agccgaagga tctgaacggc gcgctcaaga ccgcgctgcg cgcgtatccc gacggcgtgc
     4801 tcgtcgagga gtacatcgac ggcctcgatg ttgcggtcgg tttcatcgat ggcgtcggcc
     4861 atgacgacgg cctgctcacg cccgtcgaga tgtcctacga cgcaccgggc gagaacgagc
     4921 gcttcaagat ctacgactat cgactcaaga acgtcgaccc gaccaaggtg cagtaccgct
     4981 gcccggcgaa cctgccgcgc gacgtcgcgg cgcgcctgcg gcaaatctcg cacgaggtcg
     5041 tgcgcacctg cgggctgcgc gacatggcgc gcctcgattt ccgcgtcaac gcggagggcc
     5101 gcatttacct gctcgaggtc aacgcgctgc cggcgcttgc cacgtcgtcg tcgctgttcg
     5161 cggccaccgc gcaggtcggc ctgacgtatc aggcgacgat ccagtcgatc ctcaatgcgg
     5221 ccgcgctgcg ctcgggcctg gcgacggcga gccagctcgg cgtcaccaag cagcgcaagg
     5281 cgcagccgat ccgcgtcggc ttcacctaca acgtcaagcg ctcggccgac ggcgacgatg
     5341 aagccgagtg ggatccaccg gagaccatca tcgcgatcgc gaacgcgctc gcgcgccagg
     5401 gtcacatcgt cgtccacctc gaagcgacgc ccgacctgcc gagagtgctc gctgaagccg
     5461 acgtcgacct catcttcaac atcgcggagg gcgtcgaagg ccgtaaccgc gaggcgcagg
     5521 tccctgccct ctgcgagctg ctcggcattc cgtacaccgg ttcggattcg gccacgctcg
     5581 cgatcgcact cgacaaggcg ctcggcaaga aggtgctgct ccagcacgac atcctgacgc
     5641 cgaagttcca gctcatggaa tccgcgcgcg agcgcatcag cgcggacatg aagtacccgc
     5701 tgatcgtgaa gcccaacgcg gagggctcgt cgaagggcat cggttcgacg tcgatcgtcg
     5761 acgacgagga agagctgcgc gcggcggtca aggactgcgt cgagcgctac cgccagccgg
     5821 cgctcgtcga ggagtacatc gcaggccgcg agttcacggt cggcctcctc ggtgacaagc
     5881 gcccgcgggt gctgccgccg atggagatca agttcaagaa gaacgacccg cgcccggtct
     5941 acgactacac ggtcaagcag gactgggaag agtacgttta ttacgagtgc ccggcgaagc
     6001 tcaccgaggc cgagcaaaag gcgatggaaa agatcgcgcg cgcgacgttc tgggcgctcg
     6061 attgccgcga cgttgcccgc gtcgacatgc gcatggatgc ggaaggccgc atctacgtgc
     6121 tcgaggtcaa cccgctgccc ggcctcacgc cggactactc ggacctcgtg ctgatcgcca
     6181 aggcgagcgg catggaatac gaccagctga tcgccgagat catgcagggc ggtctccgcc
     6241 gactgcgcga gaagaagcgc gaggagcgtg aagagcgcga ggcgcgtgag cgcgaggccg
     6301 ccaagaacgg cgacaaggac aaggccaagc gcgtgccgaa gctcgacaag gccgatagcc
     6361 gcgaaggcaa cggtcgtgcg gaggcgcgcg agaacggtca acgcgagggt agcgggaacg
     6421 gcaacgggaa tggcaatggg aacggcaacg ggaacggcaa cggcaacggc aagagcgacg
     6481 gcaccgtgcg cgcggtgaag cgcgagcgtt cggagccaac cgcggctgcg gccaaccgcg
     6541 agcgcgatcg ggacagtgag cgcaacgaac gcgcgaagac cgatccgccg gaccgcgacc
     6601 cgtcggatct gtcaccggcc gccggcacgt cgacgaactg atcgtccgcg caagcgaaga
     6661 ccgcctcacc gaccgcgacc cgtcggatct gtcaccggcc gccggtacgt cgacgaacgg
     6721 aggttgccgc gcgtaccccg cgagcagcta gcccgagcga ggtacaacct cgaggtggac
     6781 atcagctccc ttgatccgtc gacgaacgga ggttgccgcg cgtccgccgc gagcagccag
     6841 cccgagcgag gtacaacctc gaggtggaca tcagctctct tgatccgtcg agcgacgccg
     6901 accgcgagcg ttgcgcagcg cttctggtgg ccgagtttcg cgatatcgca cctgacgcgt
     6961 ggccaaccct cgcgacggcg cgcgaatcgg tcgacgagtg cctcgcgctt ggtgtcgtgc
     7021 gcgtcgcgcg cgagaaccac gacatcgtcg gttggatcgg cgggcatcac acgtacggcc
     7081 gcgtctggga gctgcacccg ctcgtggtcg accgagcgca ccagcgacgc gggatcggac
     7141 gcgcgttggt ggcggacctc gaacgcatcg tagcggggcg cggcggactg acgctcgagc
     7201 tcggcagtga cgacgaggtc gggctcacgt cgttggccgg cgtcgacctc taccccaacc
     7261 cgctcgatca cctgatgcgc ctcgaggacc gaggcggtca tgcattcgtg ttctacgcga
     7321 ggtgcggatt cgcggtcacc ggcgtcacgc cggacgccaa tgcgctcgga caacacgaca
     7381 tccacatggc aaagcgcgtc ggcgagcgct agcggatcgc gatccatgcc ttctgttgtc
     7441 cgctgccggt acgtcgagga acgcacggac gaatcgcgat gagcgcccca ggctaaccgc
     7501 ggctacggcg cagttgattg gcgctcgtgg actgagcatc gaagccgcag ccgcgctgcg
     7561 cgagcgtgcg ctttgaccga cgacgacgaa ctgtttctac gtggcatgcg gtagtcgccg
     7621 tcgccgatca cacgcgaccg catcgcgacg cggcggcacg ttgcgtgctt tgcgtgcgac
     7681 ccggaggtga tcgatgccaa ggtttatcga cattcacacg ggtatgaagg gcgtcacgaa
     7741 gcagcagcta tcgatggagc atgaaaagga catcgaggcg cagaagtcag agggcgtgag
     7801 gtttttgcat gcgtgggcgg acccgcagtc gggcaaggtg ttctgtctgt cggaggggcc
     7861 gagcaaggac gcggtccttc gcgtgcataa gaaagcggga cacgcgaccg acgaaatcta
     7921 cgaggtgccc atcgaagtgg agtagtcgct cgcgtcgtcg atcgatcgtc atcgatcgat
     7981 tggtcaattc gagtgctcgc acgtggtcgg agtagtgcgg tcgaccccgt acgaggtgaa
     8041 cgccgcgcat tcgagaggat gcgcggcgtt ctttttgttc gcgcgttacg gcgtcgccgg
     8101 tgccggcgct ggcgctggcg ctgcgggcgg agcggacggc ggtgccggct cgaggtcgac
     8161 gccgagcgac ttgcacttct cgatcagctc gggacgcgag gccttcgcga tcgcgttgat
     8221 ccagcgcttc gcgagcgccg tgttcttggt ctggcaggca gccaaggcgc acatctggag
     8281 cttctccgcg ttgaacacga cctctttcgt gcaggccgtg ttcgtcttcg cgaagtcatc
     8341 cgctgcgaac agctcctcga tgcccttggc gggttcgggc tgcttcttct cgaccggttc
     8401 cttcttttcg gccggctctt tcttctcggc gggttccttc ttgtggaccg gctctttttt
     8461 cgcggcttgc tcgcccggat ccgccttctg gccagggtcc ttcttcgtgc cctcgcccgg
     8521 atccttcttc tcgcccggat cgttcgcagc gccgtcgccg ggcccgttct gcgcgacctg
     8581 cgcagccgcg tcaggcgttg cgaccacgac ggcttgcgca tcgaccggcg cgacggcggc
     8641 gaccgctgca tcgatcgcgg gtggcgctac ctgcggtgtc gcgtgcggcg acgggttcgt
     8701 aggcggcgcc acagcggtct tgggcgtctg cgcattggcg gacgtcggct tgctgccgcc
     8761 acgcagcttc atcacgacga ggaagacggc tgctacacat gcaaacgcga ggaccggtac
     8821 gatccagccg gaacgccgtg gcgccggcgg tagcgcgatt tcggccgtgc tcgcacgcgc
     8881 gtggtggacg cgctcggtgc gctccgcctc ggtctcggtg tgctcgccct cgctctcggc
     8941 gcggtcttcg ctgttgcgag cgtgcgctgc gtcgcgagcc gccgcttcac gcgcgaacgc
     9001 ctcgcgggtc cgcgccgctg cttcggccag gcgagccgct tcgttgcgtt tgcgcgtgac
     9061 gatgtcgtcg tccttgtcgg ccgctttgtc gtcggtgagc tttgcggtgg cgggcggcgc
     9121 ttcgcgtgac tgcgccggtt ggtcgtcgac agagaccttc gcctgcgcca acacgatcag
     9181 ccccgacgtg cccttgttgc cgagctcgcc gctcgcgctg cgcgatccgc tgagctcttc
     9241 gtcgacgaca accttgtcgg gctcgttgtc cttcaccggc tcgggcaccg attgctgcgc
     9301 cggcgctttc acgagcgcga ccctcgcaac ctcggcaggt ttggccggct cggtcttcgc
     9361 gagctcggcc gccgcggcga ccgggtggtg accgttcgtg ccgtttggct tgacgaccgg
     9421 atcggcgacg cctgtgagac gcacggcctc ttcgatcaca gcgatcggcg cttgtgcagt
     9481 cgttcgggtg tgacgatcgg gagcgccgag cggcggcagc gcggtcgacc gcgtatggcg
     9541 atcgtctgct ggcggcgtcg ccgacgcggc gaccgcacca ctcgtcagat gaccttgctc
     9601 ggcaccaggg gcgcctgccg cggcctgcgc cgcaagcggc gggttacgcg tctctttctt
     9661 ggcacgcgcg cgagcgagga tgcgatcgcg aatctgctcg aagttctcgt tcgggttgtc
     9721 catcagctcc ggcggcacgg tcggtttgtc ctggcgacct gtcgagctgc cggcgcgcgt
     9781 cgcacgcgcg gtgatgagcg ttgctgcgct gcgaatcgca tcgagctgat cgtcgaccgc
     9841 ggtgtggctt ggtgcggtga cgtcgtcggg aatctgttcg ctcgccacga gcagtggctg
     9901 cactgcatcg ggcgcgctcg gatcgaccgt ggggtccgac ttcgtgccga accacagccg
     9961 catcatgcgc gacagatccg cggtcgacag caggatgctg agcttctgcg cgagcgcttc
    10021 gatcgtcgcg agtagctcgt cggcgtcctg gaagcggtcg gcaggatcct tcgcgaggag
    10081 cttcagcacc atcgcgtcga gctcgggcgg tacgtcgtag cgcttggcgg acggcttcgg
    10141 cggctcctcg gtctcgatcg cgtccttgat ctcgtcgtcg ccacccgtgc gctggtagag
    10201 cggatcgagt gtgagcactt cccacagcac gacgccgagt gcgaacaggt cgctgcggcg
    10261 atcgagcggc tcgcccctgc actgctcggg cgacgcgtac ggcgacacct tgccggggac
    10321 acgctcgccg gccttcgcga tgccgaagtc gacgagcttg acgatgccct cctggctgac
    10381 cagcgagttg gtcggcgaca cgtcgccatg cacgatgccg agcggctggc cgtcggtgcc
    10441 gcggcgctcg tgtgcgtgat ggaggccggc cgccatcgcg gacgcgatgg tcagcaccgc
    10501 tcggactgga atctggatct tcttgctgcg cgcgtggtgc atcagcgact gcagcgtctc
    10561 gccgttgacg aactccatcg cgaagaagta cgagccgccg agcttgccga cttcgaacac
    10621 ttgcgcgacg ttggggtgct gcagctgcga cgcgagccgg gcctcggcca agaacgcttg
    10681 gacccgctcg acgtggctcg tcacggctcg tttcaagcgc ttgagcacga cgaaccgttc
    10741 gacggcgcct gcccctttcg cccgggccag gaagatttcg gtcgtctcgc cttcggcgag
    10801 ctttccgagc acctcgtagc tgtgcgcgtt tgcggtgggt tccgcgtccg acacggcctc
    10861 gattgtacct gaccggtggc cacggctcac gccggggcga tccttggcta cccggtgctt
    10921 acccgcgatt acttgtccgg acgtacccca cgctacactg cggggtgtga aggtcgcgac
    10981 gcgtatcact gttgcaacgg cggttgtcgt tgcactcgcg agcgcggctt acgcgtactt
    11041 cgaccttcgc gcgcgcaaag ccgagcgcgt cagctcgctc gcgggcgagg cgaagtcgat
    11101 ggcagcggtg ctgcggcccc agctcgaaca gcaggtctcc gcgtttcggt atccaagtga
    11161 cgagcagtac aagcagtggc tcaagaccac cggctggacc attgacgtcg tgcccgtgcg
    11221 cgccaccacc cccgccgaca tgtcgcggca agccggctgg ctgcgcgtga tggtcgatca
    11281 accaagcaga acgttctcgg aggtcgtcgg cgatcggtac tactacgcga tctcgctcgt
    11341 gcagcgggta gcgcgcgatg ccgactcgga accacagccg gtcacgtccg ccatgctcga
    11401 gctgtcgaag tcgaccgacg tgctgaccgg cgacgatgtc ggccgggcga tcacgttgat
    11461 cgtgctgatc gtcgtcgtca ccacgctgat ggtcggtgcg ctcgcgagtc ggttcgtgtc
    11521 gcggccgatc acgaagctac tcaccggtat cgacgacgtc gccaaaggtg acctctcgca
    11581 cgtcatcctc gccgaacgcg acgacgagat cggtcagatc gcgacgcgat tcaacgagat
    11641 gacgttctcg ctgcgcgagt cgcgcggtga gacgcagcgg cagaacgaag cgaagctcgc
    11701 gctcgagcag cggctcggcc agaccgagaa gctcgcgacg atcggccagc tcgcagcaga
    11761 gatcgcgcac gaagtcggca cgccgctcaa cgtcatcgcc ggccgcgcgc gatcgatgca
    11821 gcgcaagtcc aaagatccag aagtcgtcga gaaaaacgcc gaaatcgtcg ccgagcagac
    11881 cgcccgtatc acgcgcatca tccagcgctt gctcgacttc acgcggcgca aggtcggcgc
    11941 gacaggcaag gcggagatca acctcaacga gctgtcgctg accacgatgg agctactctc
    12001 cggtcagttc tcggcggcaa aggtgcgcac gcggctcgag cgcgccgagg gtctgccacg
    12061 tgtcgccggt gacgcggacc gcctccagca ggtgatgatc aacctgatgc taaatgccgt
    12121 gcaggcgatg cccgatggcg gctcgctcgc ggtcgagacg aaaagtgtgc gccgcacccg
    12181 ccccggcctc gagggcagcg cggagcaacc gttcgtggcg ttttctgtca aggacaccgg
    12241 aatcggtgtt cccgaggaga ttcgcgacaa gatcttcgac ccgttctaca cgacgaagga
    12301 tggccaaggt ggcaccggac ttggcctggc ggtctgctcc ggaatcgtca aagaacacga
    12361 cggttggatc gacgtcgagg acaacgacgg tggtggcacg attttccgcg tttaccttcc
    12421 cgcctgattc gtcgtcggct gtggcagtat cgagcgagca gtgcagcggg tgcatatcga
    12481 gtacatgggt gacagcgtcg agctgcccct cggcgaaacg gtggtcggcc gcgacgtcgc
    12541 atgcgcactg cggttcaacg atccttccgt atcgcgccgt cacctgcgtt tcatccgccg
    12601 ctcgaacgag gtgtttgtcg aggacctcgg ctcgagcaac ggcacgctgc tgaacggacg
    12661 cgcggtcgca gcaccgctgc gcatcgtcga tggcgacacg atcaatgccg gcagccgcgt
    12721 gctgaccgtg cgaatcagcg atggtgatga cgagcagccg tcgacgctcg tgctcaagac
    12781 gttcaacgcc gcgagtgaga tcaagcaggc cgcgagcagg gccacgctgc ggaacttgcc
    12841 gacggtcaag ccggcaccgc aagccgcgca tcagcgctgc ccgcggtgcg cggcaccggt
    12901 cagcgagctc gacgaagagt gcgcgaattg ccactaccac tggggcagct tcaggccgat
    12961 gagccgcacc gacgtgcggc cgaatccgat cgagcgccgc cgccacgaac gccaggtcgt
    13021 cgagctccac ctcgtctaca cgtcgagcga gctcgagatc gaagcgctgt cgcgcgacct
    13081 ctccgagagc ggcgtgttcg tgtgctcgca ggtgctcgat ccgatcggca ccgaatgcga
    13141 gctcaagatc ctccccgacg gctgcccgcc catcgacgtg cgcggggttg tccggcgcgt
    13201 cgtcgagcgc gagattgccg gtgatccgat cggcctcggc gtcgagttcg tcggcgttgg
    13261 cgagccgcag ctgacctgga tccgcgcgac gatcgagaag ctcgacgccg gcgtcagcta
    13321 gcgctactcc atcatctcgc ccgggtagta gcgatagccg ccgtacccgt agccgccgta
    13381 tccgcccgat gcgccggcaa gcttggtcac gatctgctcg gtgcgatcgt cgaggtcggt
    13441 gccgcgcacg cgcagctggc tcgccgccgc cgccttgagc tcctccgacg agcccggatc
    13501 gttgacgatc tgggtcagcg tcgtgtcgac ctcgggacca ccgttgtacg acgacgagat
    13561 cgccgcacgg gccacgttga cgtcggagtc gcgcatcagg ttcgcgagct gacggctcgc
    13621 gttcgcatcg tccatctggg cgaggccgct gatcgccgca atgcgctcgt ccggcgtgcc
    13681 actgcgcgtc gcgttcagga ctgcctgctg cgcgcgctcg ccgccgatct ggccgagcgt
    13741 cgacagcgcc atcgagcgca cctgcgggtc ttggtcgcgc atcaggttca tcagcacgtc
    13801 ggtcgaccgc tcgtcgggat tctgcgacag cgacgagatc gccgcgacgc gcacgctcgg
    13861 atccttcgac tggatcgcct tctcgatgag gcgcttcgcc tcgttcgtgc cgttgctcgc
    13921 gagcgcccac acgacctggc tcgcgttgcc cgcgtccttg ccgttcagga tctcttcggc
    13981 gagcttgagg ccttcgggct ggccgttctg caccagctgc tgcatcacct gcatcttcac
    14041 ttgcgggttg ttgcgcgcag cgccgagcag cgccgccttg acgttctcgg tcatgcccat
    14101 ctggccgagc gcaccggccg cgaccgcctg cagctccttg tcgctgccgg tgagcgccgt
    14161 gatcagcgcc tgccgcgagt cctcggtgcc gaggcggccc atcacgctcg ccgcgtacga
    14221 cgcttcgtcg cggcggcccg agaacagcgc gtcggagagc agctgaccga ccgcgggatc
    14281 cgccgggtgg ctctgcgcga gcatgtcgag cgcctggacg cgggtctggc cgcgcgcctt
    14341 gctcgcgatg tcgaggagcg catcgaacgc cttcggcgtg ccggcgtcgc cgaggatccg
    14401 catcgcttcg tacttctcgt tgcgcgagcc cttctgcgcg agctcgatcg tcatcgtgat
    14461 cgcctcgggg ttgccttcct tcacgaggcg cggcagcacc tgacggcgct cggcactcga
    14521 gccctgcttg acgacgctga gcagcgcctg ctccacatcg tcgccctgca tctgcgccag
    14581 ctgcgcgagc gcgccggtca tgctcgcgcg atccgacagc gccgcgtcga tgagcagctc
    14641 gcgcgcttcc tgaccgccga ggttcgcgag cgcgtttgcc gccgagctcg ccgcctgccg
    14701 gtcaccaatc ttcaagatct cgccgagcgt ctgcacggcc ttgtcgccgc cgacctcgcc
    14761 gatcgcgttc accgccgccc accgcgcgtt catgccaccg tacagcgcgg cctcgcggag
    14821 caccggcaag ccatcctcgc cggcgtggcc gagtgccgac agcgccgcgc tcacgagctg
    14881 cggatcgccc gccttcacga tcgtcttcag cttctcgagc atcggcttgt cgatcgtgtc
    14941 gatcgacgcg agcgccgccg cagcgacgcg cgagtccggc gcatcgacga gcttgcgcag
    15001 cgcgagctcc gacgcctgcg tgccgacgat gccgagcgcg tacacggcgc tcgacgcgac
    15061 cgagtaatcc ggcaggttcg cgagctcgat caggcgtgcc accgagttgt cggtgccgag
    15121 cgtgccgagc gcggagacgg cggcattctg cgcgggatcc ccgttctggc gcgagagctc
    15181 gagcagcacc ttctccgctt gctcgctgtg cgtcgcgccg aggttcgaga tcgccgcgta
    15241 gcgtatcgtc ggccggtcgt ccttcacatg agcgacgagc acgtcgacgg ccttctgcgt
    15301 accgatctga ccgaacgccg ccagcatcgc ctcgggcacg ccgcggcgcg gatcctcgag
    15361 cagcggcgac agcgtctcga tcgcgtcgtc gtcgccgacc atgccgagct tgtcggcgag
    15421 cagctggatg tcgcgcacgc tcttcgccgc gaaaatgcga tcgacgagat cgcgctgcga
    15481 cacaccgatg acgtcggggc tgacgtggat cggcttgcgc agctggatcg gaagcgtacc
    15541 gcgcggcgtg gaagcgagct tcgcgggcgc gttgtgagcg gcgctgccgc gatacgcgtg
    15601 caccgtcgca acgccagcga gcgcggcgaa cagtgcacag gtcagcttcc agcgggtcag
    15661 agtggtcaca gggctgtctc ctcaggtctc gtcaggtctc gacgtgtctg tgtcaaacga
    15721 acgctaggag gtaacgatcc attccgacgg agccgtcagg ttcctgtaag gaaccgcgcg
    15781 aaagcacggc aaaagcacga aggaaggtcc gcgtgcaacc ggcgtagcga agcgctgtcg
    15841 gcggggctgg aatacgcccc ccaagaagga gcgttctggc cgcttccggt cgcacgaaat
    15901 cgtgcgcgag cgtcacgggt gcattgcggg acgctcccga aagaaccggg aatgatcacc
    15961 gaggctgcgc gtggtcgcaa gcgcccagcg agcggggacg cacgttcttg cgtgaggatc
    16021 ccgatgcggg cacttcatcg attgacgcac tcctcgacgt ctctagactg aactcatgct
    16081 gacggaagag cagaccaatg agctacgagc caggctccag gcggacctga ctcggctcgc
    16141 agatcatgcg cacgcgggtc tggagttcac gatggaccgc gaccgcgatc gggttggccg
    16201 cgattccatg gacgagtcga ccgaagagga gctgtattcg acgcagcttc gcctgcacga
    16261 ccgggagacc ttcctgctga acaagatcca cgaggcgatg cgccggctgg acgccgggga
    16321 gatcgacgag tgcgaagaat gctcggaacc tatcggcttc aagcgcctga tggcccgccc
    16381 agttacgacc ctgtgcttcg agtgcaagac cgcccgcgag caggtcgaag ccgacgaacg
    16441 gcccgaatag gcgggaagtg aacggcttgc gcgggtgatc ggtcttcgcc tgggaccgga
    16501 aaagccctgc actttgctag cgttcgcgtt cctctggtac tatcccttcc gtcggggatg
    16561 cgcgaccgtt acacgatcac cgagcgactc gaccacggtg gtatggccga ggtgttccgt
    16621 ggcgttgcgg agtcgatgga gggcttcaag aagaatgtcg ctatcaagcg catccttccg
    16681 aacctgacca agaaccagaa gttcgtgtcg atgttcctcg acgaggcgcg gctctcgctg
    16741 ttcctccaac acgcgaacat cgtgcaggtc ttcgacatct cccgcacgcc cgacaacgcg
    16801 tacttcctcg tgatggagta cgtcgatggg tgcaatctca agtcactcat cgagcggcag
    16861 aagcagaagg gccgccggat cgagatctcg cacaccatct acatcatgat ggaggcgtgc
    16921 aaggcgctga actacgccca catgctcgag cacccggaga cgaacgagcc cctgggcatc
    16981 gttcaccgtg acatctcgcc gccgaacatc cttctctcga agaacggcga gatcaagctc
    17041 gtggacttcg gcctcgcaaa ggccaacacg cagatcgagt cgaccgacca gggcgtcgtc
    17101 aaaggcaagt tcagctacct gtcgcccgaa gccgcaagcg gcctcgacgt cgaccaccgc
    17161 gccgacatct tcgcgatcgg catcattctc tgggagctgt tcaccggccg tcgcctgttt
    17221 tacggcgaca ccgactacca gacggtcgag ctcgtgcgcc aggcgcgcgt accgtcgatt
    17281 gcggcgctca atcccgaaat cgagcccgag ctcgaagcga tcgtgcgcaa ggcactcgcg
    17341 aaggaccagg ccgatcgcta ccagaacgct gccgacctcg gcgacgcact cgcgcagtac
    17401 ttgttctcgc gccgcatgaa agtgacggcg cgcgacgtct ccggcctcgt gaaggacacg
    17461 caggtcgaga tgatgcgcaa gcgctcgacc gagccgaagg aatcgctgat cgacgcgctc
    17521 atcctcgacg agatgcagaa gatgacctcg ctcgtcgacg gcgagaaaga cgccggcgac
    17581 agcggttcgg aaggctcgat gagcctcgat ccgatgaagt tcgtcgacac gtcgaactgg
    17641 gcaagcgaga tcgcgcacag cggcggtcag cccaagcgct ccggccgtgc gagtggcgcc
    17701 cccggcaaca agccgcgcgt gacgtcgccg ccgacgccga gcccgggcga catggaatcg
    17761 ctcgaagcga tgctcgaacc cgagcgcacc ggcgttcacg gcaaggccga caagaagacg
    17821 ccgtgggtgc tcatcatcat cgtgatgctc gtgctcgcag ccggtgccgc cgcagccgtc
    17881 gtcatgatga tgacctaagg cgcgcagccg aagagagcgc ctgatccgtg cacgacctcc
    17941 tccgaaccag ggtctgcgct cgaatctcgc gtgaccgatc ctgggtgccg gaacatttgc
    18001 gtcgccgaaa ggtgccggca gacagtgccg tccacagcgg cgcgatctca tcgcgcccca
    18061 ggtggcaccg tttcgaggga cgcgcagccc gccccctgtg ccagggttgt tgtcgcatcg
    18121 cgtgggttga agcccgcgct tgatgcaccg gggtaacgag caccgaatgt caggcactca
    18181 gtacagcgag aagttccggg cccgcatggt cgagaagatg gccggcccga atccgatgag
    18241 cggcgtgcag ctggccgcag aaacgggagt cccgcagccg acgctgtcgc gctggctccg
    18301 ggaagccact accctccgtc ggaagatggc gaagcggcag gatgacgagg agcagaagct
    18361 tgtggctgcg gacgcgccgc agcgtacggc cgaggagaag ttctcgttgg tcttggaggc
    18421 ggcggcagtt ccggaagccg agctcggcga gtggctgcgc cgcaaaggcg ttcacgctgc
    18481 tcagctcgag gagtggcgcg ctcaggcgat ggccgggttg accggcacgg agaaacgcag
    18541 caaggagtcg gcgacggacg cgaagcacat ccgccagctc gagcgcgagc tcgcgcgcaa
    18601 ggacaaggcg ctcgccgagg cggcggcgtt gctcgtactc caaaaaaaag tccaggccat
    18661 ctggggggac gcggacgacg acaccgacga gaagaaagac aaatgatccg gacgctcgtc
    18721 gacgaagcgg ttgctgcagg tgctcggcgt gaggctgcct gcaaggttgt cggccttcgg
    18781 gcgcggacgc tgcagcgctg ggagcacacg tccgacgaca atcgccatgg tccgaagcac
    18841 gcaccggcaa acaagctctc cgacgctgag cgacgcaagg tcgtgatggt ggcgacgagc
    18901 gcggagtttc gcgaccagtc accgaagcag atcgtcccga cgctcgcaga ccgcggcgaa
    18961 tacttggcct cggagtcgac gttctatcgc gtgctgcacg agaacggcat gcagcaacac
    19021 cgtggtcgct cgcggccacc gatgccgcgc ccgcgtgggc acgcggcgta cgcaccgtgg
    19081 cagctcgcgg cgtgggacat cacgtatctg cgcagccacg ttcgggggca gtttttctac
    19141 ctctacctcg tcgaggatgt gtggagtcgc aagatcctcg gctgggacgt gcacgacgtc
    19201 gagtcgaccg acttcgccgc ggaccttgtc gagcgcatcc gcgccgaagc caacgccgaa
    19261 ggcgtcgatc ttcgtggctg ggtccttcac tcggacaacg gtggtccgat gaagggcgcg
    19321 acgatgctcg ccacgatgca caagctcggc atcgtgccgt ccttcagtcg accgcgcgtc
    19381 tccgacgaca acgcgtacgc cgaggcgcta tttcggacgc tgaagtactg cccggaatat
    19441 ccggcgaagg gtttcgcgac ggtcgaagca gcacgagctt gggttgctcg ctttgtcgcc
    19501 tggtacaacg gcgagcacct ccacagcagc atcggcttcg tcacgccgtc tgctcgtcac
    19561 gccggcgatg acgtcgctct cctcgcggct cggcgacgca cgtacgaccg agcgcgcagg
    19621 cagcacccga cccgctggtc acggcacgcg cgagcgtggg cccggcctga gatcgtgacg
    19681 ctggtccccg aggacctgat cagtctgacc agatcggatc agatcgccga aaaccggcca
    19741 gccgcattaa gactcacgcg acaacaacct tgattcctac cgcccgtcac tcgagtacga
    19801 gtacgtgcac gtgcacgtga cacgagtacg tgcacgtgca cgtcggatcg attaccgcgg
    19861 cattcagggt gtgcagccga ggtcgagacc gagcttcgcg acttcgttgc cggcgagcag
    19921 gaagtaaccg aggccgtagt cctcggtcga caggatcgag accggttgcg catcgtcggg
    19981 accgtcggcg aggccgccac cgagaccttg cacgtagccg agctggcgat ccgcgcgcac
    20041 tgcgatcgtg tttaggccaa gccatgcgcg ctcgacgacc ggcaagtacg tatcgcgatc
    20101 gagcaggccg ttattgatgc cccaggcaaa cccgaacgtg aacagcgatg tgccactcga
    20161 ctcgggcccg gggtgatctt cgggatcgaa caggctgacg ttccagaagc cgtcgtcgcg
    20221 ctgaagtggc gcgatcgccg cggccatcgt ccggaaggtg gcctcgtagt ccgggcggaa
    20281 ccggctgtcc gcggggacaa acggcagctc gcgtgcgaac gatgcgaaca cccatccgtt
    20341 accgcgcgac cagaagatct tcttgtcgtt gggagtcttg tgctgcggaa aacggtgttc
    20401 gaggtccgta taccagaggc cggcgtccgg atcgaacagc tggatgacgt cgcgcgcgaa
    20461 cacgaacagc tggaacgcgg tctcgaagta gcgctggtcg ctcgtgagcg atccgatctg
    20521 cgcccacgcc ggggctgcgt ggaatagcgc ccgggtgttt tgccaggagt cacgacgcac
    20581 gtcgcgaatc acccaatcga gcgacttcgc gagctcggcg gtgcggaccg ggtcgggaac
    20641 gatctcgtag agctcgaagt agacctgccc cgccgcctga ttgtcgggat tgcgagtgtc
    20701 ggtctcgccg atcagctccc acttgttgat gtcgccgaag cggaacgcgt agtcgacgta
    20761 gattggctcg ccgacggtct cggcggccgc gaggtccccc atgaagtagt cggcgtgctg
    20821 ccacgacatg ccgcgcaccg gcgccgggtg cgtcgcgatg aacgcgttgt tgagctcacg
    20881 catcacgacg gcagggcacg gcgtgccgac gcgcaggtcg ggcgcgatcg ctgtcgggtc
    20941 gcaggccgtc aacgccgccg cacctgccaa ccaacgacac acctgcatgg gcaagtgtgg
    21001 atctgaagcg ccctagcggc gaagcgcttt gaggtctttg agctcttcgc cgttcggcgc
    21061 gatttgcgta agccgattga tcgcatcggt gagcaccttg cgctcaccca ggcggctcgc
    21121 cagcttaccg acaagcagcc aagcgcccgg atagtcgggg ttgtactgga ctgcacgctg
    21181 agcgaactgg aacgacttgc gcggatcctt cgcgatgtcg gcggcaaata cgtacgtgtc
    21241 gacgagcgac gggtcctgat cgatcacgag atcgaagaac tgttgcgcga ccgcgttgtt
    21301 ctgctggatg tacgcgacga cgccgagacc gaagtccgcc tgcgcgactc ggcgcggtga
    21361 tgccttctcc gccttgaggc ccgcctttgc gtccttgtac gcctgctcgg cctcggccag
    21421 cttctcggcg gcaatcagtt gcttgccctc gacgatcttc gcttctggct tgttcttgag
    21481 gcgctcgggc gcgagcttct tcaccttgtc ggcgagcggc ttctcgtaga tgccgacgtc
    21541 ggccgcgagc aggaacgtct cgccgtcgct accacagccg tcgagcgcgc gcgtcagcgc
    21601 agacgcagcg accgggaagt ccgatttgcg cagcgcgagg cggccgcgtt cgcgatcgag
    21661 cttccagcgt tcgacacccg acaacttgtc ggcgctatcg agcagctgtt gcgcgtcgac
    21721 gtggtcgccg acgagcatgc gcgcgcgtgc ggtctcgatc acgagctgcg gcgtcggccg
    21781 ctcgcccgca tcgtgcagca ggctatcgag cgtctcgcgg ccgccgttcg gatcgcggtc
    21841 ctcgatcaac agcagcgcat attcgagccg tgcatcctgg ctgccgctcg cggccgctgc
    21901 cgcataggct gggcgcgcgc tgcggtagtc accaaggtcg cggtagatcc gcgcgagctc
    21961 gagttgcgcg cgtgcgagat cgaagccggt cgcggtttgc acgagcttct cgagcaggtc
    22021 ctttgctttc tcgcgcgcgg ccggatcggc gctgcggcgc agcgtggcgg catagaccgt
    22081 cgtgagcgcc gggctcgacc ccttcgggtt gtagcgctcg gcagcgtgct tggttgccgc
    22141 atcgacttcg cccgtcgaga gctcgaccca cgtgcggccg atgctcgccg gctcgtggtt
    22201 cggaaccttc ttgagcgccg catcgaagtc ggcggccgca gacgccggat cacccgccgc
    22261 gagctgggcc tggccgcgca cggcgagcgc gtcgggcagc atcatcacgt cggcctgctt
    22321 gctgatgatc tcgaccgcgt cagcggcctt gccctgggcg agcaggatac gcgcgagcgc
    22381 gatccgcgag cgcttgctgg ccggatagtc cttgagcgac ttgtccgcgg cgttgcgcgc
    22441 gatcgaaagg tcgccgacgg cgtacagcgc gtcggtgaag tcctcggcga accgcacgtc
    22501 gtcgagattg atcgcagcgg cggtcttgag atcgttgcgc gcagccttct ggtcgttgcg
    22561 catgtagttg atcagcgcga gcgcgacatg accgaacgcg acctgcgccg gggacacgcc
    22621 atgctgctgc tcggtgagcg ggcgcttgcc ctgggtgacc acgcgatcga gctgcgcgcg
    22681 gacttcgttc gccatcgcgc tgccggggaa gatccggccg ctcgcggcga ggatatcgcc
    22741 acgtgcgatc accgcggccg gatgatcggc cgtgatcgcg aagaccttgt cgagcgtcgc
    22801 gagcgcttcg tcccagctgt acgcggccgc gtaggcgcgc gccagaccga cgccgtagag
    22861 cggccgcatc tccttgtcgg tcgccgcctt gagctgcgtg atcgcggtct tcggatcgcc
    22921 ggcgagcagc gctgcttggc ctgcgacgta gtgcgcagcg gcatcatccg cggacgcgga
    22981 gagcgcagcg tcggccgcgg ccttcgccgc cttggcatcg tccatcgcga gtgcgaggta
    23041 tgcggtggcc agtgtgccgt cgaggccgcc ctgcccggca agcgcgtcga cgtctgcctt
    23101 cgcgtcgacg atgccgtcgc caaactcgta ggcgaccaac gcgcgagtgc gcgcgagcgc
    23161 agcacgattt ccaagcgtgg ccgatgcgcc gacgatgtcc gcgaggctct ctcgcgcgtc
    23221 gcgccagccc ttccaggtgt cgtctttcgc gaggtcggtc gcacgctggc gagccgcgac
    23281 gatctgtttc gcgagccgca tcgcgcggat ctgaatgcca gcgaagacgc cgccgccgat
    23341 caacacgacg ccgacgacga cccagatcgc gacctgcgcg cgcgaccggg ccttgcgcat
    23401 gccggtcttc ggccccgcct tgccaggacc gacgacgctc atctgatcga gcgccatcgc
    23461 cggattgggc tcgggctggc ccgacatcaa cgcgaggatg cgaggatcga gctccgatgg
    23521 ccgcgcggtc ggctcgttcg cagccgcgat cgcgcgtgga tccaacgcgc gtggatccat
    23581 cgccggtccg acggtggcgc tcgcccacga gggcgctggc atgccttcgg agaacagatt
    23641 ctcttggtgc agcggcggtg gtggtggcgg cgcgaccgcc atcgtcgggc ggttcgcgct
    23701 gaccgcgggc accgagaacg gcgatggccc cggtgcggct gccatcgtcg gcagtgccgc
    23761 ggcgatcggc ggctgcggcg ccggcggcag tggcgcggcg ggcggcatcg agccaacacc
    23821 acgcgcggtc atcgtcatcg gtggttgcgc cgacgcaggc ttcgagcgtg gcggcggcac
    23881 ggtcttgccc ttggggcgtt gcgacgcgaa tggcgccggc ggtccgccga tcacaccgga
    23941 cggcatcgcg acggcgttgc gcacctcgct gcgtggcccc ggcacacgcg cgcccggctt
    24001 gtcgaatggc gaacgggtct cgccgagatc ctcgtcggcg tcatcgtcga agtcgaagcc
    24061 ctgcttcgcc gacttcttgc cgatcgcggt gcccttctcg ccatcgccgg ctttcgcggt
    24121 cgggcgcgcc ggattcacgc gcgaggacac gaccgcgccg cgctcgccac cgatctttcc
    24181 gggcttcgcg gctttcggtg gcgctgcgag atcatcgaag tcgatatcgt cttcgacctc
    24241 gacgccctcg agctcgggat ccacttccac ggtgcctgtc cgatcgcccg cgtcgaacac
    24301 gcccggcggc ggcgtcggat ctggcgcccc gaccgcgcgc ttcttcgacg aacggcgcgc
    24361 gccacccggc gattgcatcg acgtcggccg cgtgtacaag ccctcgggct cgggcgcgtc
    24421 gtcgccgctc gagtgtcccg gataatgctt ggtatcgccg gcgccaatga agccgaccga
    24481 cggatgcgtc accgacggac ggtgcgccga cccgttctcg gcttcgccga gtagctgaag
    24541 gacgcgcggg tcgccgggag cggcctgcct ggctttgtgc agaacctcga tcgccggtgc
    24601 gacatcacct ttgcgcagca gcgcttcggc cttgaggatc tgggactgga tcgagctgtg
    24661 atcgagctcg agcgcgacgc gcatctcggc gagcacttcg tcgtagcgct tgagcgcgag
    24721 cagcgcttgg ccgagcacga cgcgtccttc gaccgttgtc ggtcgcccga gcaatccaag
    24781 gcggcatact ttcactgcct cttggaattg cccggcggcc acgagtgcct ggccgcggct
    24841 gacgaagtcg ctctgcgaca cttaccgtcc cgggatgtta cgccatcccg ggtccagtcg
    24901 gcgaacgacc ggggcgatgt cggcgttatt cttgggtctt gctcagaggt tcctgtgccc
    24961 ctagaccgac cggcgaaccg accggttcct cgaggccggg catctcctgc cccttgaacc
    25021 gtgcttcatc gaccatccac caggtcttgc cgtgcttctc ccaggactgg agcgactggg
    25081 tctcgcgcaa gaggccctcc gactcgcggt accacgacat cttgatctgc acggtcgctt
    25141 cgcggtcgct cttcgagtcg atcctgagca cgtcgtactc ggtgatcttg agatcgtgcg
    25201 cgcgatcgtc tgcctgctcg atgaacgtcg cgcgctcctt cggcggcacg tgaacggcag
    25261 caaccgggaa ccgctcccag cggacaccgt cgttgtattg gcgtaccgag tcggcgagcg
    25321 tgtcacccgt cttcgccgac gcgcatcctg ccagagctgc aagcaccatc acgaaccaga
    25381 tcgctctcat cactcacctc cgcccggcgc gcgcttggtg cgcacggccc ggattgcatc
    25441 gaggtcacgt ttaccgagct cgcgcgtcgc gacgagcatg acgatgaacg tcaggccaac
    25501 cactgccgct tcgacgagcg tcatgagctt gccgtgcagc ggcaggaagc ggccgacgac
    25561 gagcgccgct gcggtcgcaa tgccgatgcg cacgaggctg atcatcggta ggaacgcgcc
    25621 gagtcggttg cgcatgatcc agccgccgat gaccgcgccg acgatcatcg agccgcccgt
    25681 caccgctgcc gcggcttcga gcacgcgacc gctctcgacg accatcggaa tgacgatgaa
    25741 gttgccggcg gctgcgattc cgagcgtcag cgccgccgtc gagatcgcgg ctcgcgacat
    25801 cccagcgccg ttcaagatcg tgcccgcgat cgcgaacacc gagaacgcga cgttgccgag
    25861 cgcgagcact gcgagtgcgg cgccgccctg ctgcgcgtag tcgggcgcgt agacgagacc
    25921 gaggatgtcg acgggattcg cggccatcac gaccgcgatc gccatcgcga acatcagcga
    25981 gtagcgcgtg gtcaccttga tgtagcgctg cgtcgtctcg cggtcatccg cgaacgtcga
    26041 gtgcgagacg agcgggaaca cgacgaacgt cgccgcgatg atcgcttggt acgagagtcg
    26101 cgctaggttt tggacggcag cgtaatagcc gacctgcacg tcggcgagga tgctcggcgt
    26161 cgcgtggtag cccgagacct tcggtgccca cggcacgact tgatcgacgg cggcggcaag
    26221 ctcggccttg tgcgtcgcga agtattccgt ggtgagccgc ttgagcagcc aggtatcgac
    26281 gaacatcagc gcgttgaaca gcgcgaggta gatcgcgacg ccgatgaaga aattgatcat
    26341 cggcttcacc gggatcgttt cgccgtcgtg tgcggcttta cccggcaacc cgatccaggt
    26401 catcgcgatc atcaagatcg cgccgaccgc ggcgacccag ccgccgacga cgccgatcac
    26461 accgagaccg gccatcgcca taccgatgag accgatgctg cgcatcgtcg cgaacgtgat
    26521 gtcgagcccg gcttgcttct ggaactggcg ttggccgttc gcggtgccga caaacacggc
    26581 gtagaacgca ttgccaccga cgatcaagcc cgcgagcatg agcggcgcgg tcttcgacat
    26641 gtcgaggaac agccacgaca ccagcggcgc cgcggcgatg aacaagatcg cgacgacgag
    26701 accgagccgc acgtgcatgc gcaggccggc gagctggacc ttgcgcgcct tctccggctc
    26761 tgccgcggcg aacttactga ccgcctggat cgtgccggtg accagcacgt tgttgaccgg
    26821 cgagacgagc gagttgatga agctgtagct gccccacgcg gcgtgggaca ggatcgccgg
    26881 cagcctgacc tggacgacca ggccgacgaa catgaagtag aacttcgcga acgcgatgta
    26941 gaggacgccg cgtcccgcgc ccgttgcttt cggctcgttg gacatccgcc gtatccccgc
    27001 gtaacacgct ggatcggcag cagcaactta cttacttacc gaagcgctta tggcaggccg
    27061 atgtgcgctc gtgtgctgcg cgggaacggt taattgctgg tggcggcaag gttacgcacc
    27121 gggtactgca gcgctgcatc aatctgcggc aggccgtatg atggatgaga tccgtttggg
    27181 gaacgggttc tgtggaggta catggccgag ggtcgagtgc gcgggttcag catcgccacg
    27241 aagtgcgcga acgtcgctga gctgatcgag aagttccgag accgggtgga cgaggaatcg
    27301 atcctcgtca ccacggtcga gtctcgtgac atcggaaccg agtgcgcatt cgcgatcctg
    27361 ctcgccgaca agaaggtcgg tctcgctggc acgtgtgtcg tgcgcgacgt cttcaccgac
    27421 ccgaacaatc cgttcaagcg cccgggcatg cggctgcaga tcaagcgact cggtcccgag
    27481 agccagaagg tttggaccga gcttgtcgcc aagcgcatct cgccgcgcaa gatgacgcag
    27541 gcgctgccgg tgatcgcgat gccgtcgatg ccaggcaaga agccgcgcgt cgcgacgatt
    27601 cctcccgtcg gtgaccgccc gtcgtcgccg gcgctcactg cgaagccacc cgttatcgcg
    27661 ccgccggtcg ccgccaggcc cgttgtcgcg cctccggtcg ccgccgccaa gcctgttgtt
    27721 gttgcgccgc ctgtcgtcgc gaagccgccc gagactgcgc cgcccgtcgc tgcgaagtct
    27781 ccggaggtgc cacccgtcgt tgcgaagccg cccgaggctg cacctcccgt cgttgcgaag
    27841 tctccggagg caccgcccgt cgtcgctccc aaacccgtca tcgtgatgcc gcgcgcgaaa
    27901 ggtcgcaaca cgggcgagat gctcgatgtc gtagccgacg cgccgacgcg tcgtccgccg
    27961 acgccaacac cgcagcgtgt gcgtgacgac tcgcaagagc cgctcgcacg tgctccacgc
    28021 gcaacgccgt tcgacctcga caacctcgca aagcactcgc cggcggtatt cgacgcgaag
    28081 cagccagacc tcacgacgcc cgacgcgaag aacctcgacg agcaggaagc ggagcggaag
    28141 gcggaagcga agaaactcga agcgaagcag gcagcggcga agccggtcga gggcacgcgc
    28201 acaccgggct cgccgtacgt gctgcccgca aacccgctcg ccaacatcgc cgactcctcg
    28261 ctcgaaggct acgtcgacgt tcggctgacc gaggcgttcg gcggtgacgc gccggcggca
    28321 ttcccgcagg gcacgctcga cgatcctccg gtcgagccag agccaccgat ggtgctcgcg
    28381 ccgccgccgc cgatcgacga gatcgctgtc atcctcgcgc ggccgccggt cgacgaagtc
    28441 ccggtaacgg gcagcgggcg cgcgcgaccc gcgtcggtga ccgcgccgct caagccgatg
    28501 atcacggcga agccgccgac gcgtacccag gcgccgacga agcgcgacgc tccggtcgcc
    28561 ggcgtacgcg agcgccactc cgagccgctg ccgttgccac cgccaccagc gctgccgccg
    28621 atggctgcga cgacgcccct gccctacgca gcgacgaagt taccgaacct caacgttccc
    28681 gacgcaaacg atcgcgtgcg cgggccgctc ctcaagcgcc gcgtgcgcaa tcagctgctg
    28741 ctcgccgtgc tgctcgttcc attgctgggc gccggcgcga tggtcgcgta catgcagctc
    28801 gcgacggcgc cggcagcgac agtgacgccg ctcgcacccg ccgcaacccc gccgctcgac
    28861 gagccgctga ccacgagcgt gccagtcgaa cccgcgccgc cagccccggc gatcgccgat
    28921 cccgcgccga cgttgccgac gaggccgcag ccgatccatg cggtgctcgt tcgcacagcg
    28981 ccgatcgccg cgcgggtcac ggtcaatggt cgctcgttcg gcacgacgcc gacctacgtc
    29041 aagatccccg cgaacacgcc ggtggagctc gtgatcgcgc ggcccggttt caaaccagtg
    29101 aagtatccgt ttacgagcaa gaccgccacc gaccgcgtgt tcgtgcggct cgagcgggcg
    29161 agagggggac gatgacgcca agcgtacgtg ggttcgtggt cgcgacgaag tgcgagacca
    29221 tcgaggagct cgtggagaag tatgcgccgc ggtccgacga ccagtcgatc tttgtcggca
    29281 tcgtcgaaga gcgcgtgctc ggtgccgagt gcgcattcgc gatcctgctc gccgacaagc
    29341 agccggcatt cgcaggcatc tgccaggtgc tcgaagtgtt tcgcgacgac aacaacccct
    29401 accagcgccg cggcatgcgg ctcggcatcc gcaagctcgg catcacgagc gagcgtttgt
    29461 tcgccgacat ggccgcgatg cgcgcgaaca agcggcggtt cgcacgcggc agcacggcgc
    29521 cgcccgaagc cgatctcgac ggcgttccga tcgtcgtcga tgacgccgac gacgagatga
    29581 gcgactcgat tccgatcgat ctcgaagacg agctctcggg accagcgcgc gcgcgtagct
    29641 agtcgggtag cgcgaggtta cggcgcgggt agctggcgcc gatgctcgtt gaactgcgcg
    29701 cgagccgtgc gtgatagtgg ccccatgcaa cgcattctgc ggggactatg gctaccgatc
    29761 tgtcttgcat ctgccgcgtg cggcaccgag accgacgagc agctgaacga aaccgaacag
    29821 gaagtgatcg tcggttcgct ggcggcacgc ttcgacgaca ccgtgcgcgg tggcggcgtc
    29881 gtcgcgcaag gcgcgagcct cgccggccgt gaggacgccg tcgcgaatac gaccgcggcg
    29941 atcacgatcg caggtatccc cggtggttcg acggtgcagc gcgcgttgct ctactggtcg
    30001 atcaatggtg gtatcgacac caccgcgacg atcaacgcgg tgaatgtaaa cggcgtccag
    30061 ctgggcaccg gtggcagtac gtgctggggt acgaacttcg tgaccttccg cgccgacgtc
    30121 acggcacagg tgaccggcaa cggcgtctac acgatcggcg gactgccgtc gtcgacatcc
    30181 gcaaccgccg ccgacacgaa cggtgccgcg ctggtcgtcg tctaccagaa cccgacgtcg
    30241 ggcaatcggc gccgcgtcat ggttcgcgac ggctcgatgt cgaccgatgc gaccggcgac
    30301 aacgtgaccg ataccttcac cggcgtcgca gcgccgctcg cgagcgcggg ccgcttccac
    30361 ctggtcgtcg gcgatggcca gtcgttccct gacggcaatc tgatcttcaa cggcgtcacg
    30421 ctcggcaccg atcagttctc cggcagcgac ggctcgttgt tcgatacgag ctcgtacaac
    30481 gtcaacgtgc cggcggcgct cgtaaacgcc acctggtccg agcagacctc gagtgactgc
    30541 ctcggcttca gcacagcggt gctcgactgg aacgtcgcga cctgtggtga cgccgtgcgc
    30601 accggtggcg aggcttgcga ccagggcgac acgacgaatg gcgatggctg ttcgtcgacg
    30661 tgcgatgtcg aggccggctg gagttgcacc gcgacgaacc cgagtgtgtg cacaccgaac
    30721 tgcggcgacg gcattcgcgc cggtgccgaa cagtgcgatc agggcaacac gacgaacggc
    30781 gatggctgct cgtcgacgtg catgatcgag ccaggctatc agtgcaccgg cagtccgagc
    30841 gtttgtacga cggtctgcgg cgacggcatt atcgcaggcg ccgagcaatg cgatcagggc
    30901 aacacgacga acggcgatgg ctgctcgtcg acgtgccagg ccgagcccgg atggaactgc
    30961 gccggctcgc caagcgtgtg cacgtcggtc tgcggcgatg gcatcgtcgt cggcgcggaa
//
LOCUS       DASUFX010000558        13158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_88399,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000558 DASUFX010000000
VERSION     DASUFX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88399"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFV99_22395"
     CDS             <1..454
                     /locus_tag="VFV99_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062144.1"
                     /translation="DVWAMGTILYELATQKVAYTGETLAATVVHITSGKPPPITTLRP
                     DAPPAFVELVDRAISTDPARRIVTIEELRAGLKNALEPRLRAATPVQGVGPLPRASSP
                     AIVARASSPAISVQGVTTLPAKRSLAVWLIGTAALAAILATVLVLALH"
     gene            679..2016
                     /locus_tag="VFV99_22400"
     CDS             679..2016
                     /locus_tag="VFV99_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062145.1"
                     /translation="MTKVRYLHVVVLATVVALVPARDALGWRMREHRAIGAESYIAAC
                     NRLTPLKDRDPQTAVRYDIACGNLNVQSFLYGQGTSVGGDFLADSDEFRSTFGAALAV
                     DRGNYLRLALRNSTHFHPMVTREWGDLHEVAIAGALAASQKQGVALVDSFEQVFYDSA
                     FADHFLQDSFAAGHMGFNRPASSAGAAHAYHDEWNKRGRMVSNRRGAVWKTFGDNLLD
                     IKENAESRKHVIAAATDSVYSVLAAFVLGEYDGTSDLAVWNEVPFTIEDPELFPSLFR
                     LFAGTQKLTRPELLPLLAVKRPAVKDQILGIWTSYSFPFDSWGHPSGVVVFGGNLIIP
                     GLGLRMEAGVGFGFEGSFTEPRFAVDAGLVRGLGITVDGLFSHELDLGSLLLIDGDVD
                     ATLRLSLRTNLEAGDQLLRVDVGPSYNVNADQFGLYVGLGYARVLSAAGGGFF"
     gene            2045..2680
                     /locus_tag="VFV99_22405"
     CDS             2045..2680
                     /locus_tag="VFV99_22405"
                     /inference="COORDINATES: protein motif:HMM:NF018297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1131 family protein"
                     /protein_id="HEX5062146.1"
                     /translation="MRWLVLAALCVGCKGKKPPPKHDDARIMPADAAVADAAVDAAPD
                     AWQMSTTITSDGVGPITADTNDADDFERLMPGYSAKSEHHEAEDYSYDEILVTKNRLQ
                     ILRAVVQDKSLFKVEVDDATFTTASGVSVGTPVAELAAKIADIKCVYETYDPEADAER
                     VDRSLRCGSEKLPRVLFEIDYEKFKGPEGTVSTKTIGKRKVVQIVWLADKD"
     gene            2697..4193
                     /locus_tag="VFV99_22410"
     CDS             2697..4193
                     /locus_tag="VFV99_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX5062147.1"
                     /translation="MVSITDLAFSDRFVRALPADIRADNRIRQVMQACYSRVEPTSVP
                     APELLVLVPEVAALLGLDPTPSRELVDVLAGNRVLPGMAPYSACYGGHQFGTWAGQLG
                     DGRAITLAEVEARGETWEIQLKGAGPTPYSRMADGRAVLRSSLRELVCSEAMHHLGIP
                     TTRALSLVLTGQPVQRDMLYDGHPADEPGAITCRVARSFLRFGSYEIHASRDDKETLR
                     KLVDFTLERYYPHHRGDIPAFFDEVMQRTAWLIAEWMRVGFVHGVMNTDNMSILGLTI
                     DYGPYGWLEPFDLDWTPNITDASGRRYRFGNQPAVAHWNLAQLARALAPLVDTLDPLR
                     KTIDAFPDTFSAHYRHTMLRKLGLAGHRDTQRDDDELLTSLGELLVATKPDYTLFFRY
                     VQTDVERAFYSPLEPAQRELLAAWLARYRARNPRNADAVNPLYVPRNYLVQEVIDATE
                     RGDRTQLLELLEVLRSPYTEQPGREHYAGKRPDWAASKPGCSMLSCSS"
     gene            complement(4186..4695)
                     /locus_tag="VFV99_22415"
     CDS             complement(4186..4695)
                     /locus_tag="VFV99_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062148.1"
                     /translation="MITALERSPENLKLNDEQKQTLVGLWLLKKLDLEPKDGGMELPV
                     VLPSELGPLDEYLQTLAVQGHVEINRKKARYELTKQGIAYLGEVIDEAAAMVEDLDEL
                     ETADAIAELRARRADLMRARFLWGWYEGEFDDLVLFQERRGADSVERMWAFYLTGDDF
                     WRELAKDFS"
     gene            complement(4692..5126)
                     /locus_tag="VFV99_22420"
     CDS             complement(4692..5126)
                     /locus_tag="VFV99_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062149.1"
                     /translation="MRELLHLDEERAAPDPNYAGYGWARVRELWLDERRVDDALVVAV
                     HSADDSEPIADDVELEFELPGAQPVSVLASLFLAKWLPRLPHTNALVLALCNPHRATL
                     APPSDATLYYANGDVESWVDHDRDRGDRIQLTSEHGWVRRDS"
     gene            complement(5162..5887)
                     /locus_tag="VFV99_22425"
     CDS             complement(5162..5887)
                     /locus_tag="VFV99_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062150.1"
                     /translation="MISKTKLLASALILALGTAGVGCESDHDRVDRWATTENTNVKID
                     WDKVNQAYRDASGPEDLEKRVNEIYEGNEVISIAVKDQDDKTQIVTGFFDKNANGTVD
                     ESEKIFTIKREPTADNTAQYQTTGYGPYAGYHSPFFSIVSGMVMGSMISNMFSPRYVP
                     MTYVTPHQRLADLHYERSTYRAQNPERFKASKSGRNYGGKASKPSSTGRSWGRPSGGG
                     RSGGGRFGLARSGRTVRPERLAA"
     gene            complement(5972..7576)
                     /locus_tag="VFV99_22430"
     CDS             complement(5972..7576)
                     /locus_tag="VFV99_22430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062151.1"
                     /translation="MRFAAATGLAIGLIALAGCASFEDPFAEDAEDGFVVEDGKADDF
                     FSLQATEFIVSGTGRVVAEEGATEARVRKLIGFEHTAITWFLNQYLVDKERDGMHSDP
                     NADYGGFTAMVKNGAYEDFRITQRNATTWDFEFQQTIAGRKDLMRKLPLNASNEFSIE
                     IGKPTNDEMEHDSEWYRKDPWDAWNPATVPAAKKETLTLTIKQDTKSSDGWWDYRRLF
                     EDGTLTVDVHFGYDYNPEAAHLADSKAFYSWLVGKGFKSPVPSYDKYTRTSGPLTRTL
                     DADGKTIKVAVRFFYPRPGTVTDPNTDAGGKQLERDMFASLKSSDVIVFSGHSGPLYG
                     FALANWDKTDEGDVDDSELATAELATGKYQIVLAEGCNTYMLGPTLLKNPYKMGKDID
                     VITTTSFSVSYSPVEDFLGRLLELDSQGRHRPRTMAQTLEDLDLYSADEDSHTMYGIH
                     GIDDDPKLHPYANVENLCKSCSSNAGCGGVGNACVTVGTSGRRCVAACTADSGCPASY
                     QCKKVASASTSSIYGSYCVPATRSCQ"
     gene            complement(7715..11206)
                     /locus_tag="VFV99_22435"
     CDS             complement(7715..11206)
                     /locus_tag="VFV99_22435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062152.1"
                     /translation="MPDDERTRRWQRLDWTGTREIDPIHMLRSAINMVRAAPRDPEAR
                     RRLRGVAAEQGNWEELALLLADEARAAVERPELAAAFYEELADVHENLDQPLEVIGAM
                     EAVIAFDPHNPEHHDRLAWLYRRAGASVKAAESFERVAVLAKDDRARAALRAAGRLYR
                     ESGRLEQAIEVYRNVVDRRPSDIEAWRALDELLTEMKRWREAAEVRGELAGRANGVEK
                     AVLLRSQARALEQAGEAARAAELVASAAGHAPEDVSGLVDLATVLAREGRTTEAIDVL
                     TQRIEQALREGATDTNVAAMRLRLVEVLDATGDRVSAQRVLDQLLESLPEYVPALQKL
                     VQFGAQDPDPRVHAEALLRLAAVDDGPDMLGLLVEAARRYRAAGDLRRAAHTFERATE
                     LSPDDDALRAELQDAQTALVVEIAAAEAGAGDSHAAERSLRKILLSHPMHLDANLALA
                     DLLTKTNRAAAAIDHLRDTLADAPEDTEPALLAKLVHRVAVITRTLGDADEAHQLLHE
                     AHHLDRKNLLITLALGESCFGRKLWRETAIHLGSLAEHPDARKHRSGVGAGLVKAAQA
                     EARALKPQNAPKHYEAAVRIDPECAPAWHALAELATEAGDIQRAAECLEREANATKDA
                     DARLRLFDALGDLARDVLGDFDRAERCWAQVAQMNSASVLKKLVAVQRRRGATIARAE
                     TCERLAALGSDERARKELTEEAAEAYAAGRDYSRAQVLAEYLVSNYPHDIDAVGCAAR
                     VALTAGSNDRAANWLRRALHAWEASGNRGDGDPRRADLWRRLGDAEKARHNERNALDA
                     YQRAVSVAPESDAALGARRGLIELAASAGRSANTSRLALVEAEQDPNDVIASARELAA
                     AGSFEEGRAMYELARTLGVELLAEDEQFLDENPPRPMASDEAYAVALDDRERRALVDD
                     EHEGPLADLMLLLGEAANLVCPDAKTALDQEALFDARRVASSSEAAVVAMFPQIAKAL
                     GGPPTLLYADPTRAAADLRILLASPPVVVIGPRLSALRARSRGDAELDSDAELRFRIG
                     RVIEQSRPHRLFVAGVEPDLFARLVAGLWHAFGKPDVEPTREVAREAERLRHALPVQL
                     RRRMTERLASTPAGALDLQTYRDACERAADRAGMLACGHTGIAIHLAGGVERAAHLVR
                     FAASQKYLAARRKLRRR"
     gene            11280..11834
                     /locus_tag="VFV99_22440"
     CDS             11280..11834
                     /locus_tag="VFV99_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062153.1"
                     /translation="MVRRWMKVLVLVAGFLGVLGFVEPFFEIGDVEVSAYRIASRADS
                     LVVRLTREPIYADEARFEVGGITVEPRATRSMIPYYFASAIVFLLVGFIALVRGRLSG
                     FVALPSLCAALFALGGWMREYRFDRDLVRDGHPALLAVGAKLLLLSGLLGLIGSVVVL
                     VKREAEVPKPPPPPPEIPEARVVT"
     gene            11888..>13158
                     /locus_tag="VFV99_22445"
     CDS             11888..>13158
                     /locus_tag="VFV99_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062154.1"
                     /translation="MKLRGIGIATGLFAAAGSASAGGLFVPGSGAVSTSRAGAAVASA
                     DDGEALSVNPAGIAKTQGVKITFSATLIQYFMSFSRRGSYDPVTNPDGTTRPYNGTDY
                     DTVENDPKPPLGIGKWQPLPVLVASWDLGGRVPGLAIAGGLYTPSGYPFRDMSQGYDF
                     KRDVSTDTIAPPPTRYDIMTQESALLLPSIAAAYRITPDLDVGARFTAGNLKTKTQVI
                     VWGTPANVDEDIRNDSIFTADVADGFIPTGGIGATYRIGPSIEIGANWTAPVVIKAKG
                     TAVSFKGPSTDQSKVIGPVPDAMAKCAKGGTAEEQKACISLQLPMTAQVGGRYKFLDS
                     AGKVIGDVELDLDWENWGKRCNFDSAHIVGSTCTSPGQFRVQIDSGLYVNDTFTQALQ
                     TNAVNLGLQDTYGVRLGGSYRIPLGENEITAR"
BASE COUNT         2092 a         4421 c         4296 g         2349 t
ORIGIN      
        1 cgacgtctgg gcgatgggca cgatcctcta cgagctggcg acgcagaagg tcgcctacac
       61 gggcgagacg cttgccgcga ccgtcgtcca catcacgagc ggcaagccgc cgccgatcac
      121 gacgctgcgg cccgacgcgc caccggcatt tgtcgagctt gtcgatcgcg cgatatcgac
      181 cgatccggca cgtcgcatcg tcacgatcga agagctgcgc gccggcctca agaatgcgct
      241 cgagccacgc ctgcgcgcag cgacgccagt ccagggcgtc gggccactcc cccgcgcgtc
      301 gtcacccgcg atcgtggcgc gagcctcgtc accggcgatc tccgtgcagg gcgtgaccac
      361 gctgccggcg aagcgcagcc ttgccgtgtg gctgatcggc accgccgcgc tggccgccat
      421 tctcgcgacc gtgctcgtct tggcactcca ctagttctcg agccagcggc gcatcaagcg
      481 cgcgagcacg gtgcccagaa cgcgagatca ccgcgtacgt tgaggcccgc atgcccgcgt
      541 ggctctgcgt gtgccgtccc cagcgtgatg accacgatgg cgagtcgtta cagcattttc
      601 tcgtcgtatc acgaggcgct cggcgatggg tgcgtggctc ggcacgcttg atgcctgctc
      661 gtgcgcggaa ggagtgccat gacaaaggtc cgttacctcc acgtggtcgt tctggccaca
      721 gtcgtggcgc tggtacctgc gcgcgatgcg ctcggctggc gcatgcggga gcaccgcgcg
      781 atcggcgccg agtcgtacat cgccgcgtgc aaccggctca cgccgctgaa agatcgcgat
      841 ccgcagacag cggttcgtta cgacatcgcg tgtggaaacc tgaacgtgca gtcgtttctc
      901 tacggccagg gcacgtcggt gggtggagac ttcttggccg attcggacga gttccgatcg
      961 acgtttggtg ctgcgctcgc ggtagaccga ggaaactacc ttcgcctcgc gctcagaaac
     1021 agcacccatt ttcatccgat ggtgacgcgc gagtggggcg atcttcacga ggtggcgatc
     1081 gcaggtgcgc ttgcggcatc gcagaagcag ggcgtcgcgc tggtcgacag cttcgagcaa
     1141 gtgttctacg actcggcgtt cgccgaccac ttcctgcagg actcgttcgc cgccggtcac
     1201 atgggattca atcggcccgc gagcagcgcc ggtgcggcgc acgcttatca cgatgaatgg
     1261 aacaagcgtg ggcggatggt ctcgaaccgc cgcggcgcgg tctggaagac ctttggtgac
     1321 aacttgctcg acatcaagga gaacgcggaa tcacggaagc atgtgatcgc cgctgcgacc
     1381 gactcggtct acagcgtgct cgccgcgttc gtgctcggcg aatacgacgg tacgagcgac
     1441 ctcgcggtgt ggaatgaggt cccgttcacg atcgaggatc ccgagctgtt tcccagcctc
     1501 ttccggctat tcgccggaac gcaaaagctg acgcgccccg agcttctgcc gctgctcgcc
     1561 gtgaagcgcc ccgcggtgaa ggatcaaatc ctcggcatct ggacgtcgta tagctttccg
     1621 ttcgacagct ggggccaccc gagcggtgtg gtcgtgttcg gcgggaatct gatcattccg
     1681 gggctcggcc tgcgaatgga agcaggggtc ggcttcggct tcgaaggcag ctttacggag
     1741 ccgcgctttg ccgtcgatgc cgggctcgtc cgaggcctcg ggatcaccgt cgatgggctc
     1801 ttcagtcacg agctagacct cggctcgctg ctcctgatcg acggtgacgt cgacgcgacg
     1861 ttacggctct cgctgcgcac gaacctcgag gccggagacc agctgctccg cgtcgacgtc
     1921 ggaccgtcct ataacgtcaa cgcggaccaa ttcgggctct acgtgggact tggctacgcg
     1981 cgcgtcctga gcgctgccgg cggcggattc ttctaagcga atacgtgcga acatagccgt
     2041 accgatgaga tggctggtgc tggcagcgct gtgtgttggc tgcaagggca agaagccgcc
     2101 gccaaaacac gacgacgcgc ggatcatgcc cgccgatgct gccgtcgccg atgcggcggt
     2161 cgatgcggcg cccgacgcgt ggcaaatgtc gaccacgatc acatccgatg gtgtcgggcc
     2221 gattaccgcc gacacgaacg acgcggatga tttcgagcgc ttgatgccgg ggtattccgc
     2281 gaaaagcgag caccacgagg cggaggacta ctcgtacgac gagatccttg tcaccaagaa
     2341 cagactccag atcttgcgcg cggtcgtgca ggacaagagt ctattcaagg tcgaagtcga
     2401 tgacgcgacg ttcacgaccg cgtcgggcgt gagcgtcggt acgcccgtcg ccgagctcgc
     2461 ggcgaagatc gccgacatca agtgcgtgta cgagacctac gatcccgagg cggacgccga
     2521 gcgcgtcgat cgctcgctgc gctgcggctc ggagaagctg ccgcgcgtgc tgttcgagat
     2581 cgactacgag aaattcaaag gacccgaggg cacggtcagc acgaagacga tcggcaagcg
     2641 caaggtcgtc cagatcgtgt ggttggcgga caaggactga cgaggcacac tgcgtcgtgg
     2701 tctcgatcac cgacctcgca ttctccgacc ggttcgtccg cgcgctgccg gcagacatac
     2761 gcgccgacaa tcgcatccgc caggtgatgc aagcgtgcta ctcgcgcgtc gagccgacgt
     2821 cggtgccagc gcccgagttg ctcgtgctcg tgcccgaggt cgcggcgctg ctcggtctcg
     2881 atccgacgcc ctcgcgcgag ctcgtcgatg tgctcgcggg taaccgcgtg ctgcccggca
     2941 tggctccgta ttccgcgtgc tacggcggcc atcagttcgg cacctgggca ggccagctcg
     3001 gcgatggccg cgcgatcacc ctcgcggaag tcgaggcgcg cggcgagacg tgggagatcc
     3061 agctcaaggg cgcgggcccg acgccgtact cgcgcatggc cgacggacga gccgtgctgc
     3121 gctcgtcgct acgcgagctg gtgtgcagcg aagccatgca tcacctcggc attccgacga
     3181 cacgcgcgct atcgctcgtg ctcacgggcc aaccggtcca gcgcgacatg ctctacgacg
     3241 gtcacccggc ggacgagccc ggggcgatca cgtgccgcgt cgcgcgctcg ttcctgcggt
     3301 tcggcagcta cgagatccac gcgtcgcgcg atgacaagga aacgctgcgg aagctcgtcg
     3361 acttcacgct cgagcgctac tacccgcatc accgtggtga catccctgca ttcttcgacg
     3421 aggtcatgca gcgcacggcg tggctgatcg ccgagtggat gcgcgtcggc ttcgtgcacg
     3481 gcgtgatgaa caccgacaac atgtcgatcc tcgggctcac gatcgattac ggcccgtatg
     3541 gctggctcga gccgttcgac ctcgattgga cgccgaacat caccgacgcg agcggtcgtc
     3601 gctatcgttt cggcaaccag ccggcagtcg cccactggaa cctcgcgcag ctcgcgcgcg
     3661 ccctcgcgcc actcgtcgac actctcgatc cgctccgcaa gacgatcgac gcgttcccgg
     3721 atacgttctc cgcgcactat cgtcacacga tgctgcgcaa gctcggcctc gccgggcacc
     3781 gcgacaccca gcgcgacgac gacgagctat tgacgtcact cggcgagctg ctcgtcgcga
     3841 ccaagcccga ctacacgctg ttcttccgct atgtccagac cgacgtggaa cgcgcgttct
     3901 attcgccgct cgagccggcg cagcgcgagc tcctcgctgc gtggctcgcg cgctatcgcg
     3961 cacgcaatcc gcgcaacgcc gacgctgtca atccgctata cgtgccgcgc aactacctcg
     4021 tccaggaagt gatcgacgcg accgagcgtg gcgaccgcac ccagctgctc gagcttctcg
     4081 aggtgctgcg ctcgccatac accgaacagc caggccgcga acattacgcg ggcaagcgtc
     4141 ccgactgggc cgcgagcaag ccgggctgct cgatgctgtc gtgctctagc tgaagtcttt
     4201 cgcgagctcg cgccagaagt cgtcgccggt cagatagaac gcccacatgc gctcgaccga
     4261 gtcggcgcca cgccgttcct ggaacagcac gaggtcgtcg aattcgccct cgtaccagcc
     4321 ccacagaaac cgcgcgcgca tcaggtcggc gcggcgggcg cgcagctccg cgatcgcgtc
     4381 ggcggtctcg agctcgtcga ggtcctcgac catcgcagcc gcctcgtcga tcacctcgcc
     4441 gaggtacgcg atgccttgct tcgtcagctc gtagcgagcc ttcttgcgat tgatctcgac
     4501 gtggccctgc accgcgagcg tctgcagata ctcgtcgagc gggccgagct cgctcggcag
     4561 cacgacggga agctccatgc cgccgtcttt gggctcgaga tcgagcttct tgagcagcca
     4621 caggccgacg agcgtctgct tctgctcgtc gttgagcttc aggttctccg gcgagcgttc
     4681 aagtgcagtg atcacgagtc cctcctcacc cagccgtgct cgctggtgag ctgaatgcga
     4741 tcgccgcggt cgcggtcgtg gtcgacccac gactcgacgt cgccgttcgc gtaatagagc
     4801 gttgcgtcgc tcggcggcgc gagcgtcgcg cgatgtggat tgcacagcgc gagcacgagc
     4861 gcgttcgtgt gcggcagcct tggcagccac ttcgcgagaa acaacgacgc gagcacgctg
     4921 acgggttgcg cgccgggcag ctcgaactcg agctcgacat catctgcgat cggctcgctg
     4981 tcatcggccg agtgaaccgc gactaccaac gcatcgtcga cacgccgctc gtcgagccac
     5041 agctcgcgga cccgcgccca gccgtagccg gcgtaattcg gatccggcgc tgcccgctcc
     5101 tcgtcgaggt gcaatagctc gcgcacgtct cgctcgcgaa acggtagctc gaggatcgtc
     5161 actatgccgc caaccgctcc gggcgcacgg tgcgaccact gcgtgcaagg ccgaagcggc
     5221 caccgcccga gcgcccacca ccggacggcc ggccccacga acgtcccgtg ctgctcggct
     5281 tcgacgcctt gccgccatag ttgcggccgc tcttcgacgc cttgaagcgc tcggggttct
     5341 gcgcgcgata cgtcgagcgt tcgtagtgca gatcggcgag ccgttggtgc ggcgtcacgt
     5401 acgtcatcgg cacgtagcgc ggactgaaca tgttcgagat catcgagccc atcaccatgc
     5461 ccgacacgat gctgaagaac ggcgagtggt aaccggcgta cgggccgtag cccgtcgtct
     5521 ggtactgcgc cgtgttgtcg gcggtcggct cgcgcttgat cgtgaagatc ttctcgcttt
     5581 cgtcgacggt gccgttcgcg ttcttgtcga agaagccggt gacaatctga gtcttgtcat
     5641 cctgatcctt cactgcgatc gagatgacct cgttgccctc atagatctcg ttcacccgct
     5701 tctcgagatc ctcgggaccg ctcgcgtcgc gatacgcctg gttgaccttg tcccagtcga
     5761 tcttgacgtt cgtgttctcg gtcgtggccc agcgatcaac gcgatcgtga tcggactcgc
     5821 agccgacacc agcggtgccg agtgcgagga tcaacgcaga cgcgaggagc ttggtctttg
     5881 aaatcatggg gacaacctag tgatggcgtc cccgcgatca agtgctcaaa atattcccgc
     5941 ccgatcggct agggcgcgtt acgcgagcgc cctactggca gctgcgcgtc gccggcacac
     6001 agtacgagcc gtagatcgag ctcgtcgacg ccgatgcgac tttcttgcac tggtagctcg
     6061 ctgggcagcc gctgtcggca gtgcaggcgg ccacgcaacg gcggccactc gtaccgacgg
     6121 tcacgcacgc gttgcccacg ccgccgcagc ccgcgttcga gctgcagctc ttgcacagat
     6181 tctcgacgtt cgcgtacgga tgcagcttcg gatcgtcgtc gatcccgtgg atgccgtaca
     6241 tcgtgtgcga gtcctcgtcg gcgctgtaga ggtcgaggtc ttcgagcgtc tgcgccatcg
     6301 tgcgcggccg atgacggcct tgcgagtcca gctcgagcag gcggccgagg aagtcttcga
     6361 ccggcgagta cgagaccgag aacgacgtcg tcgtgatcac gtcgatgtcc ttgcccatct
     6421 tgtacgggtt cttcagcagc gtcgggccga gcatgtacgt gttgcagcct tcggcgagca
     6481 cgatctgata cttgcccgtc gcaagctcgg cggtcgcaag ctcgctgtca tcgacatcgc
     6541 cttcgtcggt cttgtcccag ttcgcgagcg cgaatccgta gagcggtccc gagtggccgg
     6601 agaacacgat cacatcgctg gacttgagcg atgcgaacat gtcgcgctcg agctgcttgc
     6661 cacctgcgtc ggtgttcgga tccgtgaccg tgcccggccg cggatagaag aagcgaaccg
     6721 cgaccttgat ggtcttgccg tcggcgtcga gcgtgcgagt cagcggcccg ctcgtgcgcg
     6781 tgtacttgtc gtacgacggc accggcgact tgaagccctt gccgacgagc cagctgtaga
     6841 acgccttgct atcggcgagg tgtgccgcct ctgggttgta gtcatagccg aagtgaacgt
     6901 cgacggtcag cgtgccgtcc tcgaacagcc gccggtaatc ccaccacccg tcactcgact
     6961 tcgtgtcttg cttgatcgtc agcgtcagcg tctctttctt cgccgccggc acggtcgccg
     7021 gattccacgc gtcccacgga tctttgcggt accactcgga gtcgtgctcc atctcgtcgt
     7081 tcgtcggctt gccgatctcg atcgagaatt cgttgctcgc gttcagcggt agcttgcgca
     7141 tcaagtcttt gcgaccggcg atcgtctgct ggaactcgaa gtcccacgtc gtcgcgttgc
     7201 gctgcgtgat gcgaaaatct tcgtacgcgc cgttcttcac catcgcggtg aagccgccgt
     7261 aatccgcgtt cggatcggag tgcatgccgt cacgttcttt gtcgaccagg tactggttca
     7321 agaaccacgt gatcgcggtg tgctcgaagc cgatcagctt gcgcacgcgc gcttcggtcg
     7381 cgccttcctc ggcgacgacg cggccggtgc cgctgacgat gaactcggtc gcttgcagcg
     7441 agaagaagtc gtcggccttg ccatcctcga ccacgaagcc gtcctcggca tcctcggcga
     7501 acgggtcctc gaacgaggca cagccggcca gagcgatgag accgatggcg agaccagtag
     7561 ccgcagcgaa ccgcatgcgc agggtggttt gcacggccag taccagcggc cgtggcagtg
     7621 gatcccgccg gttagcgagc gtggctcacg tccctggata gccgagcggc cggcggttgt
     7681 tacaaacccg cgccgcgatt gcacgaaacg cgcatcagcg gcgccggagc ttgcggcggg
     7741 cagcgaggta cttctgcgat gcagcgaagc gcacgagatg agcggcacgc tcgacgccgc
     7801 cggcgagatg gatcgcgatg ccggtgtgac cgcacgcgag catgccggcg cgatccgcgg
     7861 cgcgctcgca cgcgtcgcgg tacgtctgca ggtcgagtgc gcctgctggt gtcgacgcga
     7921 ggcgctcggt catccggcga cgcagttgca ccggcaatgc gtgacgcaga cgttcggctt
     7981 cgcgcgcgac ctcgcgcgtc ggctcgacgt cgggtttgcc gaacgcatgc cagaggccgg
     8041 cgacgaggcg cgcgaagagg tcgggctcga cgcccgcgac gaacaggcga tgcggacgcg
     8101 attgctcgat cacgcgaccg atgcggaacc gcagctcggc atcgctgtcg agctcggcat
     8161 caccacgcga gcgtgcgcgc agcgcgctga ggcgcggacc gatgacgacg accggtggcg
     8221 acgcgagcag gatgcgaagg tcggccgcgg cgcgcgtcgg atcggcgtag agcagcgttg
     8281 gcggcccacc gagcgccttc gcgatctgcg ggaacatcgc gacgacggcc gcttcactcg
     8341 acgaggcgac gcggcgcgca tcgaacagcg cttcttgatc gagcgcggtc ttcgcatcgg
     8401 ggcagacgag gttcgcagct tcgccgagca gcagcatcag atcggcgagt ggcccttcgt
     8461 gctcgtcatc gaccagtgcg cgacgttcgc gatcgtctaa tgcgacggcg tacgcttcgt
     8521 cggacgccat tggcctcggc ggattctcgt cgaggaattg ctcgtcttcc gcgagcagct
     8581 cgacaccgag ggtgcgcgcc agctcgtaca tcgcacgccc ttcctcgaac gagccggcgg
     8641 cagcgagctc gcgtgctgat gcaatgacgt cgttcggatc ctgttccgct tcgacgagtg
     8701 cgaggcgcga cgtgttcgca gaccgccccg cagacgctgc aagctcgatc aagccgcgcc
     8761 gtgcaccaag cgcggcatcg gattcgggcg ccacgctgac cgcacgctga tacgcgtcga
     8821 gcgcgttgcg ttcgttgtgg cgcgcctttt cggcatcgcc gagacgtcgc cacaggtcgg
     8881 cgcgtcgtgg gtcgccgtca ccgcggtttc cggaggcttc ccatgcgtgc agtgcacggc
     8941 gcagccagtt tgcagcgcga tcgttcgacc cggccgtgag cgcgacgcgc gcggcgcagc
     9001 cgacggcatc gatgtcgtgc ggatagttcg acacgaggta ctcggcgagc acttgtgcgc
     9061 gggaatagtc gcggcctgcg gcgtaggctt cggcggcctc ttcagtcagc tccttgcgtg
     9121 ctcgctcgtc gctgccgagt gcagcaagtc gctcgcaggt ctccgcgcgc gcgatcgtcg
     9181 caccgcggcg gcgctgtacg gcgacgagct tcttcagcac cgacgcgctg ttcatctgcg
     9241 ccacttgcgc ccagcagcgc tcagcccgat cgaagtcgcc gagcacgtcg cgtgcgagat
     9301 cgccgagcgc gtcgaacaat cgtagccgcg catccgcatc cttcgtcgcg ttcgcctcgc
     9361 gttcgaggca ttcggcggca cgctggatgt cgccggcttc cgtcgccagc tcggcgagtg
     9421 catgccacgc gggcgcgcat tcgggatcga tgcgcaccgc tgcctcgtag tgcttcggcg
     9481 cgttctgcgg cttgagcgcg cgagcttccg cctgcgccgc cttcacgagc cccgcgccga
     9541 cccccgaacg atgcttgcgc gcgtcgggat gctcggcgag tgagccgagg tggatcgcgg
     9601 tctcgcgcca cagcttgcgc ccgaagcagc tctcgccgag cgcgagcgtg atcagcaagt
     9661 tcttgcgatc gaggtgatgc gcttcgtgga gcagctgatg cgcctcgtcg gcgtcgccga
     9721 gcgtgcgcgt gatcacggcg acgcgatgca cgagctttgc gagcagcgcc ggttcggtgt
     9781 cctcgggtgc atccgcgagc gtatcgcgca gatgatcgat cgctgctgcg gcccgatttg
     9841 tcttcgtgag caggtcggcg agcgcgaggt tcgcgtcgag gtgcatcggg tggctgagca
     9901 ggatcttgcg caagctgcgc tcggctgcat gcgagtcgcc cgcacctgct tccgcggccg
     9961 cgatctcgac gacgagtgcg gtctgcgcgt cttgcagctc tgcgcgtaac gcatcgtcgt
    10021 cgggtgacag ctcggtcgca cgctcgaacg tgtgcgctgc tcgccgcaga tcacctgctg
    10081 cgcgatagcg gcgggctgcc tcgacgagca ggccgagcat gtccggtcca tcgtcgacgg
    10141 cagccagtcg cagcagcgcc tcggcgtgca cgcgcgggtc gggatcctgt gcgccgaact
    10201 gcacgagttt ttgcagcgct ggcacgtact cgggcagtga ttcgagcagc tggtcgagca
    10261 cgcgttgcgc gctcacgcgg tcgccggtcg cgtcgagaac ctcgacgagt cgcaagcgca
    10321 tcgcggcgac gttcgtatcg gtcgcgcctt cccgcaacgc ttgctcgatg cgctgcgtga
    10381 gtacgtcgat cgcctcggtc gtacggccct cgcgcgcgag cacggtcgcg agatcgacga
    10441 ggccactgac atcctcgggt gcatgaccgg cagcactcgc gaccagctct gcggcccgtg
    10501 ctgcctcgcc agcctgctcg agtgcgcgtg cctgcgagcg caacagcacc gctttctcga
    10561 caccgttcgc gcggccggcg agctcgccac gcacctccgc ggcctcgcgc cagcgcttca
    10621 tctcggtcag tagctcgtcg agtgcgcgcc acgcctcgat gtcactcggt cgtcggtcga
    10681 cgacattgcg atagacctcg atcgcctgct cgagccggcc gctctcgcga taaagccggc
    10741 ccgcggcgcg caaggccgcc cgcgcgcgat cgtctttcgc gagcacagca acgcgttcga
    10801 acgactcggc tgctttgacc gacgcgcccg cccggcggta cagccacgcg agccggtcgt
    10861 ggtgctcggg gttgtgcggg tcgaacgcga tcaccgcctc catcgcgccg atcacttcga
    10921 gcggctggtc gaggttctcg tgaacgtctg cgagctcttc gtagaacgcg gccgcgagct
    10981 ccggtcgctc gaccgccgca cgtgcctcgt cggcgagtag cagtgccagc tcctcccagt
    11041 tgccttgctc ggctgcaacg ccgcgcaacc ggcggcgtgc ctctggatcg cgcggcgcgg
    11101 cacgcaccat gttgatcgca ctgcgcaaca tgtggatcgg gtcgatttcc cgggtgcccg
    11161 tccagtcgag ccgctgccag cgtcgggtgc gttcgtcgtc tggcacgtcc ggagcagtgt
    11221 aaagcgaacc tcgccgtcgg tgcagacaca atcggattga tctgatacga cgaaataacg
    11281 tggttcgtcg ttggatgaag gtgctcgtgc tggtcgccgg ttttctgggc gtcctcggct
    11341 ttgtcgagcc cttcttcgag atcggcgacg tcgaggttag cgcctaccgc atcgccagcc
    11401 gcgccgattc gctggtggtg cggctcactc gcgagccgat ctacgccgac gaggcccggt
    11461 tcgaggtcgg cggcatcacc gtcgagccgc gcgccacgcg gtcgatgatt ccgtactact
    11521 tcgcctcggc gatcgtgttc ttgctcgtcg gcttcatcgc gctcgtgcgt ggccgcctca
    11581 gcggcttcgt cgcgctgccc tcgctgtgcg ccgcgctgtt tgcgctcggc ggctggatgc
    11641 gcgagtaccg gttcgatcgc gacctcgtgc gcgacggcca tccggcgttg ctagcagtcg
    11701 gcgcgaagct gctcttgctc tccggcctgc tcggcctgat cggctcggtc gtcgtcctcg
    11761 tcaagcgcga ggccgaggtc ccgaagccgc ctccaccgcc gcccgagatt cccgaggcac
    11821 gcgtcgtcac gtagcgcgcg ttctatttcc catccggcga tcgacgtgat gtagattccc
    11881 gggaactatg aagcttcggg gaattggcat cgccactggt ctattcgccg ctgcgggatc
    11941 tgcgagcgcc ggcggtttgt tcgtgcccgg ttcgggcgca gtatcgacgt cgcgcgcggg
    12001 cgctgcggtc gcgtcggccg acgacggcga agcgctctcg gtcaaccccg cgggcatcgc
    12061 gaagacccag ggcgtgaaga tcacgttctc ggcaacgctg atccagtact tcatgagctt
    12121 ctcgcgccgc gggtcctatg accccgtcac gaatcccgac ggcacgacgc ggccatacaa
    12181 cggcaccgac tacgacaccg tcgagaacga tccgaagccg ccgcttggca tcggcaagtg
    12241 gcagccgctg cccgtcctcg tcgcatcgtg ggatctcggc ggccgcgtgc ctggtctcgc
    12301 gatcgccggc ggcctctaca cgccgagcgg ttatccgttc cgcgacatgt cgcaaggcta
    12361 cgacttcaag cgcgacgtct cgaccgacac catcgcgccg ccgccgacgc gctacgacat
    12421 catgacgcaa gagagcgcgc tgctcttgcc gtcgatcgcc gcggcgtatc gcatcacgcc
    12481 cgatctcgat gtcggcgcgc gcttcaccgc cggtaacttg aagacgaaga cgcaagtcat
    12541 cgtgtggggc acgccggcaa acgtcgacga ggacattcgc aacgacagca tcttcaccgc
    12601 agacgtcgca gacggcttca tcccgacggg cggcatcggc gcgacgtatc gcatcggccc
    12661 gtcgatcgag atcggcgcga actggaccgc gccggtcgtc atcaaggcga aaggcaccgc
    12721 ggtcagcttc aagggaccgt cgacggatca atcgaaggtc atcggtccgg tgcccgacgc
    12781 gatggcgaag tgcgcgaagg gcggcacggc cgaagagcag aaggcttgca tctcgctgca
    12841 gctgccgatg accgcgcagg tcggcggtcg ctacaagttc ctcgacagcg ccggcaaggt
    12901 gatcggcgac gtcgagctcg atctcgactg ggagaactgg ggcaagcgct gcaatttcga
    12961 cagcgcgcac atcgtcggct cgacgtgcac gagccccggc cagttccgcg tccagatcga
    13021 ctccggcctc tacgtcaacg acacgttcac gcaggcgctt cagacgaacg cggtgaatct
    13081 cggcctccag gatacctacg gcgtgcgtct cggcggcagc tatcgcattc cactcggcga
    13141 gaacgagatc acggcccg
//
LOCUS       DASUFX010000559         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1894868, whole genome shotgun sequence.
ACCESSION   DASUFX010000559 DASUFX010000000
VERSION     DASUFX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894868"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFV99_22450"
     CDS             <1..392
                     /locus_tag="VFV99_22450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062155.1"
                     /translation="VPMRDEQVRRYARHLMLPDVGGLGQTALMVASAKLVMRESEPDA
                     ELVAGRYLAAGGVGALVAPTASDEQRADLAAHGADTKVLGDGDGREVALAPRPAWWPS
                     TDGDATALAFWRGGAAATLWMADIVNR"
     gene            389..871
                     /locus_tag="VFV99_22455"
     CDS             389..871
                     /locus_tag="VFV99_22455"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX5062156.1"
                     /translation="MTPMATIPIPARVLAAIYQHARATFPDECCGYLVGPRDAGIDAA
                     VQCRNAQRDGEHPTHPDRDADTGFVIAGAELLAFARSLDGDRPARVVYHSHTNGRAYF
                     SEVDRQVAGAPPHYPVQHLVVGVTQGGLTEAAQFAWSDEAREFVEVARWPIDFSQPLV
                     "
     gene            889..1743
                     /gene="larE"
                     /locus_tag="VFV99_22460"
     CDS             889..1743
                     /gene="larE"
                     /locus_tag="VFV99_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX5062157.1"
                     /translation="MSSKLDALSATLTGYGRVVVAFSGGVDSAFLLKVAVDALGEQCH
                     AVTAVSPTMARSEIKASIELAAALGLGARHHVIESHELEVPGFADNPTHRCALCKTEL
                     MDVAVPIAQTLGDAPIVLGTNIDDLGDVRPGIAAARERGAKMPMVDVGLSKAEIRELS
                     RELALPTWDKPQLACLSSRFPYGTKITPERLRRVDEFEDGLRELGFRQLRVRFHDTIA
                     RLEIDADDLPRVVAPGVRDRIVALGKRLGFSYVALDLGGFRSGSLNEVLPADTLVQLG
                     GARLKRSV"
     gene            1740..2318
                     /locus_tag="VFV99_22465"
     CDS             1740..2318
                     /locus_tag="VFV99_22465"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5062158.1"
                     /translation="MKHALLAPLALLAMTAPAAAEAPYQAKKDRERGGKSTNHAAPPA
                     IGTKPGKLVNLYNLWTKDWLGVDPSDPPSIGEIDIFLRDHFTNEATKMEPKLITTVCA
                     AAEQFKSEVVDVVSGFRHPKYNLMLRKKGHQVARDSQHTHGNAIDFVIPGVALGKLHE
                     WAKAQKLGGVGIYLTSKFVHMDTGPVRFWAGD"
     gene            2332..3858
                     /locus_tag="VFV99_22470"
     CDS             2332..3858
                     /locus_tag="VFV99_22470"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX5062159.1"
                     /translation="MSVWAWLGIGFGSLLMLVAIYDVSQKRRAIIRNFPIIGHFRYWL
                     ETIGPELRQYIVTGNDEERPFSRDQRRWVYASSKKENNYFGFGTDNDPEQSGYILVRH
                     SAFPKHTPHEGEPGYDPKYSMPSIRVLGGVRGRKHAFRPTSIINTSAMSYGSLSSNAV
                     AAINQGVALCDALQNTGEGGISDYHRKGGNIIWQIGTGYFGCRDAQGKFSLERAKESI
                     ASANVKAIELKLSQGAKPGLGGVLPAAKITPEISRIRGVPLGHDCISPASHSSFSDVS
                     SMLDFIEKLADETGLPVGIKSAVGEMSFWRELATQMVKTKRGPDFITIDGGEGGTGAA
                     PLVFSDHVALPFKLGFTEVYRTFAEAGANHHTVFIGSGKLGFPESALLALALGCDMVN
                     VAREAMLAIGCIQAQRCHTGHCPTGVATQNKWLMRGLDPTLKAARLANYIVTLRKELQ
                     SLAAACGVAHPALVPLDQIDIVISSTETKSARLVYGYQDGWGLPPVHEAEQLRSVHAA
                     "
     gene            3938..4276
                     /locus_tag="VFV99_22475"
     CDS             3938..4276
                     /locus_tag="VFV99_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062160.1"
                     /translation="MTYARPDGFATIATIVAIEKLGYGFGSVGHMLYMMQQVAPGPYR
                     TAHYAFATGIMALSLMLTGMVSGQLQECLGYQHFFVFVLFASIPSVLFAAFAPFHGEE
                     DVRLQIAAAH"
     gene            4335..4916
                     /gene="orn"
                     /locus_tag="VFV99_22480"
     CDS             4335..4916
                     /gene="orn"
                     /locus_tag="VFV99_22480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX5062161.1"
                     /translation="MAGPDVLVWLDMEMTGLDPDRERIIEVATILTDGQLNEIATGPE
                     LVIHQSDEILAAMDDWNKSHHGASGLTERVRTSTVTDAQAEQQTIEFINAHVPAKDRP
                     VLAGNSIHQDRRFIRRYMPALEKRLHYRMVDVSTIKELARRWYPQLIAKQKAKKETHR
                     ALDDIRESIDELRFYKQHVFIAPPTPPAQGSNS"
BASE COUNT          871 a         1685 c         1610 g          799 t
ORIGIN      
        1 cggtaccgat gcgtgacgag caagtgcgcc gctacgcgcg tcacctgatg ctgcccgatg
       61 tcggcggcct cggccagacg gcgctgatgg tcgcgtccgc gaagctcgtg atgcgggaga
      121 gcgagcccga tgccgagctc gtcgcgggtc gctatctcgc ggcgggtggc gtaggtgcgc
      181 tggtcgcgcc gactgctagc gacgagcaac gcgcggacct cgccgcgcat ggcgccgaca
      241 ccaaggtgct tggcgacggc gacgggcggg aagtcgcgct tgcgccgcga cccgcgtggt
      301 ggccaagcac cgacggcgat gcaacggcgc tcgcgttctg gcgcggcggg gccgcagcga
      361 cgctgtggat ggccgacatc gtcaatcgat gacgccgatg gcgacgattc ccatcccggc
      421 gcgcgtgctc gccgcgatct atcagcacgc gcgcgcgaca tttcccgacg agtgctgtgg
      481 ctatttggtc gggccacgcg acgccggtat cgatgccgcg gtccagtgcc gtaacgccca
      541 gcgcgatggc gagcacccga cgcaccccga ccgcgacgcg gataccggct tcgtgattgc
      601 cggcgccgag ctgctcgcgt tcgcgcgctc gctcgacggc gatcgaccag cgcgggtcgt
      661 ctatcactcg cacacgaacg gccgcgcgta cttctccgag gtcgaccgcc aggtcgcggg
      721 tgcgccgccg cactacccgg tgcaacacct cgtggtcggg gtcacccaag gcgggctcac
      781 cgaggctgcg cagttcgcct ggtccgacga agcccgcgaa ttcgtcgagg tagcccggtg
      841 gccaatcgac ttcagccagc cgctggtgta gatgctaccc tcgtcgccat gtcgtcgaag
      901 ttagacgcgc tcagcgccac gttgaccggc tacggccggg tcgtggtcgc gttctcgggc
      961 ggcgtcgatt cggcgttcct gttgaaggtc gcagtcgatg cgctcggcga gcagtgccac
     1021 gcggtgaccg cggtgtcgcc gacgatggcg cgctcggaga tcaaagcgtc gatcgagctc
     1081 gcagcagccc tcggactcgg cgcacgccac cacgtcatcg agtcgcacga gctcgaagta
     1141 cccggcttcg ccgacaaccc gacgcatcgc tgcgcgctgt gcaagaccga gctgatggat
     1201 gtcgcggttc cgatcgcgca gacgctcggc gacgcgccga tcgtgcttgg caccaacatc
     1261 gacgatctcg gcgacgtgcg cccggggatc gcagcggcgc gcgagcgtgg ggcgaagatg
     1321 ccgatggtcg acgtcgggct cagcaaggcc gagattcgcg agctctcgcg cgagctcgcg
     1381 ctgccgacgt gggacaaacc gcagctcgca tgcctgtcgt cgcggtttcc gtacggcacg
     1441 aagatcacgc ccgagcggct gcgccgcgtc gacgagttcg aagacggttt gcgcgagctc
     1501 ggcttccgtc agctgcgcgt gcgctttcac gacacgatcg cgcggctcga gatcgatgca
     1561 gacgaccttc cgcgcgtcgt cgcacccggc gtgcgcgatc gcatcgtcgc gctcggcaag
     1621 cgcctcggct tctcgtacgt cgcactcgat ctcggcggct tccgctctgg ctcgctcaac
     1681 gaggtgcttc ccgcggacac cttggtgcag ctgggtggtg cccgactgaa gaggagcgtg
     1741 tgaagcacgc gctgctggcg ccgctcgctc ttttggcgat gacggcccca gcggccgccg
     1801 aggcgccgta tcaagccaag aaggatcgcg agcgaggcgg caagtcgacg aaccacgccg
     1861 cacctcccgc catcggtaca aagccgggca agctcgtcaa cctctacaac ctatggacga
     1921 aggactggct cggcgtcgat ccatcggacc caccgagcat cggcgagatc gacatcttcc
     1981 ttcgcgacca cttcacgaac gaagcgacga agatggagcc gaaactgatc acgaccgtgt
     2041 gtgcggccgc cgagcagttc aagagcgagg tcgtcgacgt cgtctcgggc tttcgccacc
     2101 cgaagtacaa cctgatgctc cgcaagaagg gccatcaggt cgcgcgcgac agccagcaca
     2161 cacatggcaa cgcgatcgac ttcgtgattc ctggggtcgc actcggcaaa ttgcacgaat
     2221 gggcaaaggc acagaagctc ggcggcgtcg gtatctatct gacgagcaag ttcgtccaca
     2281 tggacaccgg tccggtccgg ttctgggcgg gcgactgata gactcgtagc catgtcggtg
     2341 tgggcatggc tcggcatcgg gttcggctcg ttgttgatgc tcgtcgcgat ctacgacgtg
     2401 agccagaagc gtcgcgcgat catccgcaac tttccaatca tcggtcactt ccgctactgg
     2461 ctcgagacga tcggtcccga gctgcggcag tacatcgtca ccggtaatga cgaagagcgc
     2521 ccgttctcgc gcgatcagcg gcgctgggtc tatgcgtcgt cgaagaagga gaacaactac
     2581 ttcggattcg gcaccgacaa cgaccccgaa cagtccggct acatcctcgt ccgccacagc
     2641 gcgtttccga agcacacgcc gcacgaaggc gagcccggct acgatccgaa gtactcgatg
     2701 cccagcatcc gcgtgctcgg cggtgtccgc ggacgcaaac acgcgttccg gccgacctcg
     2761 atcatcaaca cgagcgcgat gagctacggc tcgctgtcga gtaatgccgt cgccgcgatc
     2821 aaccaaggcg tcgcgctgtg cgacgcgctg cagaacaccg gcgaaggcgg catctccgac
     2881 taccaccgca agggcggtaa catcatctgg cagatcggca ccggctactt cggctgtcgc
     2941 gacgcgcaag gcaagttctc gctcgagcgt gcgaaggaga gcatcgcgag cgcgaacgtg
     3001 aaagcgatcg agctcaagct ctcgcaaggt gcgaagccgg gcctcggcgg cgtgctgccg
     3061 gccgcgaaga tcacacccga aatctcgcgc atccgcggcg tcccgctcgg ccacgactgc
     3121 atctccccgg cgagccactc ttcgttctcc gacgtgtcga gcatgctcga cttcatcgag
     3181 aagctcgccg acgagaccgg gttgccggtc ggcatcaagt ctgcggttgg cgagatgagc
     3241 ttctggcgcg agctcgccac gcagatggtc aagaccaagc gcggtcccga cttcatcacg
     3301 atcgatggcg gcgaaggcgg caccggcgca gcaccgctcg tgttcagcga tcacgtcgcg
     3361 ctgccgttca agctcggctt caccgaggtc taccggacgt tcgccgaggc cggcgcgaat
     3421 catcacaccg tgttcatcgg ctcgggcaag ctcggcttcc ccgagtcggc gctgctcgcg
     3481 ctcgccctcg gctgcgacat ggtcaacgtc gcgcgcgagg cgatgctggc aatcggctgc
     3541 atccaggcgc agcgctgcca caccggccac tgccccaccg gcgtcgcgac gcagaacaag
     3601 tggctgatgc gcggtctcga tccaacgctc aaggcggcgc gcctcgcgaa ctacatcgtc
     3661 acgctgcgca aggagctgca gagccttgcc gcggcgtgcg gcgtcgcgca cccggcgctg
     3721 gtgccgctcg atcagatcga tatcgtcatc agcagcaccg agacaaaatc cgcgcgcctg
     3781 gtctacggct accaggacgg ctggggattg ccgccggtcc acgaggccga gcagctacgc
     3841 agcgttcacg cggcctgatt ccgcggcgac gcacacgatc tcgacgacct cgccgggctc
     3901 cgcatacggc acgagatcgc cgctctgtac gatgccgttg acgtacgccc gccccgacgg
     3961 cttcgccacg atcgcaacga tcgtcgcgat cgagaagctc ggctacggct tcggctcggt
     4021 cggtcacatg ctctacatga tgcagcaggt ggcacccggc ccctaccgca ccgcgcacta
     4081 cgcgttcgcg accggcatca tggcgctgtc gctgatgttg accggcatgg tcagcgggca
     4141 gctccaggag tgcctcggct accagcactt cttcgtgttc gtgctgttcg cgtcgatccc
     4201 gtcggtgtta ttcgccgcgt tcgcgccgtt tcatggtgag gaggacgtgc ggctccagat
     4261 cgccgccgcg cactgatcac agcgaatggg cgtggcgtac gccacgaggg tgggaatcgt
     4321 gtaacgtcca aggcatggct ggccccgacg ttctggtctg gctcgacatg gaaatgacgg
     4381 ggctcgaccc cgatcgcgag cgcatcatcg aggtcgcaac gatcctcacc gatggtcagc
     4441 tcaacgagat cgcgacgggc cccgagctcg tgatccatca gtccgacgag atcctcgcgg
     4501 cgatggacga ctggaacaag tcgcatcacg gcgcctcggg tctgaccgag cgcgtgcgca
     4561 cgtcgacggt caccgacgcg caggccgagc aacagacgat cgagttcatc aacgcacacg
     4621 tgccggcaaa agaccggccc gtgctcgccg gcaattcgat ccatcaggat cgccgcttca
     4681 tccgccgcta catgcccgcg ctcgagaagc gtctgcacta ccggatggtc gacgtgtcga
     4741 cgatcaagga gctcgcgcgg cgctggtacc cgcagctgat cgcgaagcag aaggccaaga
     4801 aggaaaccca ccgcgcgctc gacgacattc gcgagtcgat cgacgagctg cgcttttaca
     4861 agcagcacgt gttcatcgca ccgccaacgc ccccagcgca gggctcgaac agctgaccgc
     4921 ggtgagggct gcgcgacgcc accggacgcg acgtgcgtgg caact
//
LOCUS       DASUFX010000560        18110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_367441,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000560 DASUFX010000000
VERSION     DASUFX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367441"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFV99_22485"
     CDS             <1..244
                     /locus_tag="VFV99_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062162.1"
                     /translation="CPTASPPDEWELYCRDPYQDYQACDQPPDDPVDPMDPADPSDDG
                     YFGGGCNANGGASSLLLVAAVFGLRRRRGRRAISRR"
     gene            complement(228..4454)
                     /locus_tag="VFV99_22490"
     CDS             complement(228..4454)
                     /locus_tag="VFV99_22490"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX5062163.1"
                     /translation="MTKLRLFLGAALLSTSCAGSGPPANDVAAVPQQAASQSQLDKSI
                     AAMTPAKVFASDKLVKMSSLINDYFQRNPSQRSYVMTDKPLYQPGETIWFRADLRATG
                     SLVGKTDMGLNMMLMSPRGSIVAQKRVLAKSGVAQNDFALSPDIEGGEYTIQLTADDG
                     THTTKKIIVNTYEAPRLKKSLELLRKAYGEGDQVSAAIEIKRATGEAFADKALTAVVT
                     VDDIEVARVAIKTDASGGATAKFQLPAKIARGDGLLTILADDGGVTESIQKRIPIVMK
                     TMQLSLFPEGGDLVEGVAGRVYFMAKNTIGKPADIEGKIVDDRGQTVTTFASIHDGLG
                     RFELAPDADRTYHVEITKPAGITQKFDVPAAKSGGCVVRSVDDSPTKLRVAALCSSNR
                     TVMVEAVLREKKLADGAIEVQAGKPALIELPIKDDVKGVQGATRVTLFSAKQEPLAER
                     LVYHGKGQDLKVELTADKKRYSPRDPVKLKIKTTDPTGKPVKANVGVAVVDDTVLSFA
                     DDKSGRMLSKLYLEPELGATDEDPIEEPNFYFGDKPEAVAAMDALLATRGYRKFEWQP
                     VLNPPPPVDTWATSAAIAPGAGWGSAAVVQAVPPAVEAPMDLAAAEPAPQPERRAKVA
                     MKPKGNNVGLAEGAAGGGAKNLKLADRKAVNEQAKKEARWDNKAGEKQALGRARMAMD
                     KDEEWGGEGDDGLVAGWSPVRVFPVPEYKKGYEGPRTDFRETIYWNASVDTSSDGTAT
                     VSFPVSDAVTSFRAIAEGVSAGGVPGGGEAVIQSKMPLALDARLPVEVTSGDSIRLPV
                     SLTNETDDDIDADLTASFGAAFKLSNNPAGKIHLRAGEKKSLFFPLEVVATDGEGNVS
                     LGVRAAGLDDKLEKKIRVVPLGFPFELSASGTAAAGKISKHELDLSGALPGSITATVN
                     MYPSPLASMTKGMEAMIREPGGCFEQTSSSNYPNIMVLSYLATNDAADAALVQKTQGT
                     LDRGYKLLTGYETKQKGYEWFGQTPGHEALTAYGLMEFADMAKVYDVDHAMVERTADW
                     LMSRRDGKGGFLRSSTALDSFGRASEATTNAYIMWALGEAKRTKGLDKELAAQKSLAD
                     GTKDPYLLALATNTALLAQMPEAKSYVQKLVAMQGKDGSFDGAKESITMSGGESLDIE
                     TTSLATLAMIKASPNAELENQIRRAVDWLNTKRSGYGQWSNTQATILGLKALNAYSEY
                     SKQMQASGSATLIVNGVEAGTIAFEKGRKDALVWKDFASKLKPGKNTIEVKLVGQAAL
                     PYSIAVDYRAARPQSSNAAKVTVTTQLAKDHIKLGEGVKLRARVENTTKDGLPMTLAR
                     VGIPGGLTFQTWQLKELRDKGAIDFYETRPREVILYWRALAPSAKKDIDLDLLAATPG
                     TYEAPATSAYLYYTAEDKAWTAPVKINVEK"
     gene            4567..5808
                     /locus_tag="VFV99_22495"
     CDS             4567..5808
                     /locus_tag="VFV99_22495"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX5062164.1"
                     /translation="MMAAAIGALAATLVIALSWRNAWAPAVGALLGVAIAFVGGAAST
                     ADVEHAARDLWRPLLVIISIMTTAACASELGVFAYLAEWIEPRTRGPVRHAFRLVFVL
                     AALCAAVLSNDAAILMFTPVVIELLRQVYPKRHGKFVHAFAFAVFVAAGVAPLATGNP
                     MNLVVAQRAGIGFNEYARHMIPVALVGWIVAYLALAWYFRAELADEAPAKGGRLPTVQ
                     PVDGPARIVLFATAGSILAYPILAALHQPLWTVAVPTAIICTMAATRRDVPVTRILRE
                     ISWELIPFVFGVLVLATALARVGVTDSLAQIYARSSAPLPTVGAVAAVGSALINNHPM
                     ALLHSVALAGEPHSLVYAALIGGDLGPRLLPIGSLAGLLWLHTLRVRGVVIPFGLFIR
                     VGLVVTIPSLLVSLATLWLLA"
     gene            5960..6637
                     /locus_tag="VFV99_22500"
     CDS             5960..6637
                     /locus_tag="VFV99_22500"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEX5062165.1"
                     /translation="MSDRVAFLRKSYGLLGGALIVWAAASAYVFRYMPEVSFKWSRWA
                     LTGYNWFAVIGLLIGSGMLAQWLARSETSRTIQYIGLGVEVAAWTFLMQPMLWVLFMK
                     FKPAGAQALLAQGTIATLAIFVGLTATVFITKKDFSFLRGVVTVGMFAALGIIMASIL
                     FGFTLGLVFTGALIALLALKILYDTSLMMNYFPPTHYVSAALMLFGTVATLFWNIMVF
                     LMRMRSD"
     gene            6725..7699
                     /locus_tag="VFV99_22505"
     CDS             6725..7699
                     /locus_tag="VFV99_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5062166.1"
                     /translation="MSAVVAPAPLSSTDIGAVRRLEDAVATVIRGKPEAIRAAVIALL
                     SRGHLLIEDIPGVGKTTLARALAAALGGSFRRIQFTSDLLPSDIVGVSVYDQQGKIFE
                     LKKGPIFANVVLADEINRTTPRTQSALLEAMSEGQVSIDDATHELPRPFMVIATQNPA
                     EHYGTYPLPESQMDRFLLRVSLGYPNKHIERDLLKNRHGADPVAELQPVVDLSAVRTL
                     QDGVETIRMDDALLDYAMQIVEETRRHPSISVGISTRGALAWYRAAQAAALAGGREFV
                     IPDDIKGLAIPCLAHRLVLAQAHDSLGRARADSERVIADIIGRVSVPT"
     gene            7699..8796
                     /locus_tag="VFV99_22510"
     CDS             7699..8796
                     /locus_tag="VFV99_22510"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5062167.1"
                     /translation="MSFWARLRRRFRPPRRLSFTREGKLIVILSVGVGFAAINTGNNL
                     LYLLLGWLLSFIVASGILSELTLRALTVERRPPPRIYASEPFLMEVLIKNGKPNRASY
                     SIEVEDLQGKTPIDKRCYFLKIPEAKSQRTSYRHTFVKRGLYTLTGYRLATKFPFALF
                     RKSRDVEAQLDVLVYPARVSVPRPPPRAATRGDAIADRLGRRGEFFGLRERRPGDDRR
                     DVHWKSSARTGRLLVREYEDELARRVVIGVDNALPQDVRDAVTDGVLTPAFEAQVSAV
                     ERAISIAASLATAYLEVGWTVELVSRGVHVAPGTGRMHEAKIARELALLPYVGDDVAF
                     ATMPARIESVLVQPRGVPAQGRPSAGTVMEA"
     gene            8796..10973
                     /locus_tag="VFV99_22515"
     CDS             8796..10973
                     /locus_tag="VFV99_22515"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX5062168.1"
                     /translation="MRFAVAHKAATYLMITFAFLAMIAGGGVSPLFALGGIVGLVGSW
                     WWEPPNIRWEKWTWVWTTASVFALVYSVLTAIVTGDFLGVGAQFLIWLVVAKAYNRRA
                     ARDWQQMYLLAFLMLVAGSVLNADLTYGLCFLGFVISATWALTLFHLRREMEDNLLVK
                     HAADRASERVEVRRILDSKRIVGGRFFLGTGLLSFGVFLVAAIVFLAIPRVGFGFFLK
                     GRGGLTLTGFSDGVKLGGHGVIKNDSTVVMRVEIDSKYGGRDKAEIHWRGVAFDHYEN
                     ARWTRTRLAPSTRVVRYIDRPGIERYLLSWQDSAVAFDAIHDGAKQDIWLDPLDSDVL
                     FGASNPHVFEMAAIARKRKEKLQLNDELRVDHGSTVHYTVWSDVTTPSPDELRRPIGA
                     APGNYAVYLQLPPEITPQTFALARRLTDGLPTNYDKAIAIERWLETNLSYTLELKDPG
                     KTEPVHFFLFDRKKGHCEYFASAFVVLARAAGIPTRQVNGFLGGEWNEYQGYVAVRAG
                     DAHSWAEVFFPGRGWVTFDPTPAAPGGELGRGGTGWRAKLGRFIDTLRFQWSKWVIEY
                     DLVAQLSLFKDIGRALKSAAGHVRDGVLAVFRYWPIAAILGGGIAGLLVLRRRRRGPG
                     MEHGARVVKPRTRSPIAAVYDQVTKQLAKHGTRREPGTTPRELADRALAKGDPAATEL
                     RELTELYYAAEWGGRRDPGAEQRAGELAIAIRRRLRDSAKSAT"
     gene            11242..13452
                     /locus_tag="VFV99_22520"
     CDS             11242..13452
                     /locus_tag="VFV99_22520"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HEX5062169.1"
                     /translation="MTRYVLLAFLGLGASISWADMGMLPDGAQLKFNKVLLHEHNAEV
                     PAEPEIDPDSLLFYFNSAHCECSFFNYNPETADMTFFENNVAFELTVEGGSTPVTDPI
                     EIWTGTGCDTNDTTQRAADCHKVDSAGAGTVAAIQQSGAATISVPIFDVMVPKASERP
                     GCKEREQDGTVWALVDVGGTGMTNYSVSKTLKTDTKPPPLPTNFHAVGAENAVVITWD
                     SPAEGVSDVAYYQALCATAGGAPAKSNPPKARYQTSRQLCGAPSDVFLTPSDISSNTG
                     TDAGSGSIVLTEEMAQLQPAFICGESASATKNELRIDGLKNGSEYTVILLTIDRSGNA
                     TGTFFTSPLVPQPATDFWEDLHDQGSGVEGGFCLLAQTFGDDNPLTNTLRSFRDNTLA
                     DTAYGRWLIDVYYGTIGALDLHGSVALRIVAGVLLLPLVVLALLWHLLTLPGLLALAA
                     LLVLIRKRRFAQARLAAVTTVAFVALFPARAHAQSPYWETQEYTNTEAELPPGDPMRV
                     RWHAGIRLGPYVPGIDDQVNMPVGKFAGPYEQMFGGSSVLPVLDVDYFLWRGFGQLGV
                     GASLGYLGKKAHAWQAGSDPADPKRPRAEGDENKFRLFPFSLNVVYRFTYLDDEYGVP
                     IVPYARGGLAYYIWWITAPNGDFSKSCVGPNTGAMCQTTTAAGASLGVVGSVGLAVRA
                     ERVDEGAARSMRESGIEHAGFYAEYSIGKVDGFGSDKKLSVGDATWFAGVDFEF"
     gene            13574..16375
                     /locus_tag="VFV99_22525"
     CDS             13574..16375
                     /locus_tag="VFV99_22525"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5062170.1"
                     /translation="MKRVLVPALLLAACGGAKPEAVVPAPPTSSAAAPAVAAAEPAAV
                     TSTKSAPARKIRTVEGITEYQLANGLQVLLFPDATQSTVTVNVTYLVGSRHEGYGETG
                     MAHLLEHMMFKGTPTHRNVLKLLGEKGASANGTTWLDRTNYFETLPASQENLDWTLAL
                     EADRMRNASISPDDLKTEFSVVRNEFEMGENNPQAILDERIVSTAFLWHNYGKSTIGS
                     RSDIERVPVPALRAFYEKYYQPDNAVLVVSGKYDDAKALASVEKMFGSIPRPTRVLAP
                     TYSVEPAQDGERTVTLRRNGDVNVIGLAYHTVAGTSDDFPAVEAALDMLTREPAGRLY
                     KKLVETKLAASIDSTAMATHDPFLAQLSADVRDAKNVATVEKIMIEEMEGLAASKIDD
                     KAVARWRVATLKELELAMADSERIAVELSEYAALGDWRTLFAYRNRVEKVTVADVQRV
                     AKTYFKASNRTLGKFIPTKDIDRAPLTETPDINAYVKGIEGGEVKEQGEVFQATLDNI
                     EQRTTRTQLKGGIKAALLPKKTRGGKVELNLALHWGDEKSLQNKSTQADMLGSVLVRG
                     TTKKSYQDLQDLEDQLKAHIWFSTSADGLTLHIETLRDKLPAALDLAVEMLKTPSFPD
                     KELEIVRQEQLSSLEQQLQEPMAVAFQTVQQLTTKWPKGDPRYPESTTEQIAATKKVT
                     MAEIKQFYRDFVGAGYGELSVVGDFDPKALTAQIEQHFEKWQTKKPYKRLEGKAFGVP
                     GATKSVDIKDKEMTTLALSHDIQMKDTDADYAAWLMASQILGGDAASRAWMRLREKEG
                     LSYGVATWAFADAFDNVGGFGGYAIVAPQNLAKAKASLLDEIEKLTTSKVTDEELRRA
                     KETWIKEQDTGLSSDGYVIQMLSRQTFRGRTTAYTKELRAKIQAVTADDVARVAKKYL
                     DPKRLVIVDAGDVSKQQKK"
     gene            complement(16406..17416)
                     /locus_tag="VFV99_22530"
     CDS             complement(16406..17416)
                     /locus_tag="VFV99_22530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062171.1"
                     /translation="MSTRCHTCDEDLEDEARFCGVCGVSLVDENFGRTIARRYQLRQR
                     VGAGSLGAVYKAEQTGTRRKIAIKLLPGNAQHDPLATERFRREGHVLVNLRSQHTITT
                     YEFDREPDGSLYIAMELSPGRSLAHVLRHDGPLPWQTVMRILLGLCNSLGEAHAMGVV
                     HRDLKPENIIVEERPSDAYFVKVSDFGLAKILGANLRISPVGQNVGSVEYSSPEQLLD
                     RGLDARSDIYGLGVLAFLLITGQHPFAESRTFGEMVAAHIHAPAPLLRTVRADVPADV
                     ESLIACMLEKNPDRRYPSAQTLGAQISLLLSGVPAEPGATIRTDEAEEDTYLAEVPKK
                     AD"
     gene            17508..17948
                     /locus_tag="VFV99_22535"
     CDS             17508..17948
                     /locus_tag="VFV99_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062172.1"
                     /translation="MRTTVVCLVVLLFASPVRADSPSRGHVVVDALGVDARTGEKLIA
                     IVDAHDAELAKLSRERGELKSQLVASRRLDPKSLDGLLDATVANQRALAELDGRLIQR
                     VRKLVPSPKAVQLLFLLSVTEPTLPAATVSTAVCDPWTSMHRCN"
BASE COUNT         2912 a         6129 c         5848 g         3221 t
ORIGIN      
        1 gtgcccgacg gcatcaccgc cggacgagtg ggagctgtac tgccgcgatc cgtaccagga
       61 ctatcaggcg tgcgaccaac cgccggacga tccggtcgac ccgatggatc ctgccgaccc
      121 gagcgacgac ggttacttcg gcggtggctg caacgcgaac ggcggcgcga gcagtttgct
      181 actcgtcgcc gccgtcttcg gactccgtcg ccgtcgcggt cgccgcgcta tttctcgacg
      241 ttgatcttga ccggcgccgt ccacgccttg tcttcggcgg tgtagtagag gtacgcgctc
      301 gtcgcgggtg cctcgtacgt gcccggtgtc gcggccagca gatcgagatc gatgtctttc
      361 ttcgccgacg gcgcgagcgc acgccagtag aggatcactt cacgcgggcg cgtctcgtag
      421 aagtcgatcg cgcccttgtc gcgcagctct ttcagctgcc acgtctggaa cgtgagacca
      481 cccgggatac cgacgcgcgc gagcgtcatc ggcaggccat ccttcgtcgt gttctcgacg
      541 cgggcgcgca gcttcacgcc ttcaccgagc ttgatgtgat ccttcgcgag ctgcgtcgtc
      601 acggtcacct tcgccgcgtt cgacgactgc gggcgcgccg cgcggtagtc gaccgcgatc
      661 gagtacggca gtgccgcctg accgacgagc ttgacctcga tcgtgttctt gcccggtttc
      721 agcttgctcg cgaagtcctt ccacacgagc gcgtccttgc ggcccttctc gaacgcgatc
      781 gtgcccgcct cgacaccgtt gacgatcagc gtcgcactgc cgctcgcctg catctgcttc
      841 gaatactcgg agtaggcgtt cagcgccttg aggccgagga tcgtcgcctg cgtgttgctc
      901 cactgaccgt agccgctgcg cttcgtgttc agccaatcga cggcgcggcg gatctggttc
      961 tcgagctcgg cgttcggcga cgccttgatc atcgcgagcg tcgcgagcga agtcgtctcg
     1021 atatcgagcg actcaccgcc cgacatcgtg atcgattcct tcgcgccgtc gaagctgccg
     1081 tccttgccct gcatcgcgac gagcttctgc acgtacgact tcgcttccgg catctgcgcg
     1141 agtagcgcgg tgttcgtcgc gagcgcgagc agatacggat ccttcgtgcc gtcggcgagc
     1201 gacttctgcg ccgcgagctc cttgtcgagg cccttcgtgc gcttcgcttc accgagcgcc
     1261 cacatgatgt acgcgttcgt cgtcgcctcg ctggcgcggc cgaacgagtc gagcgccgtc
     1321 gacgagcgga ggaagccgcc cttaccgtcg cggcggctca tcagccagtc cgcggtgcgc
     1381 tcgaccatcg cgtggtcaac gtcgtagacc ttcgccatgt cggcgaactc catgaggccg
     1441 tatgccgtca gtgcctcgtg gcccggcgtt tgaccgaacc actcgtagcc cttctgcttc
     1501 gtctcgtagc cggtgagcag cttgtagccg cgatccagcg tgccttgcgt cttctgcacg
     1561 agcgccgcgt cggccgcgtc gttcgtcgcg agatagctca gcaccatgat gttgggatag
     1621 ttcgacgacg acgtctgctc gaagcagccg cccggctcgc gaatcatcgc ctccatgccc
     1681 ttcgtcatcg acgcgagcgg cgacggatac atgttcaccg tcgcagtgat cgagcccggc
     1741 agcgcgcccg acagatcgag ctcgtgcttg ctgatcttgc ccgccgctgc ggttcccgac
     1801 gccgacagct cgaatgggaa gccgagcggc acgacgcgga tcttcttctc gagcttgtcg
     1861 tcgaggcccg ccgcgcgaac gccgagcgac acgttgccct cgccgtcggt cgccacgacc
     1921 tcgagcggga agaacaggct cttcttctcg ccggcgcgca ggtgaatctt gcccgctgga
     1981 ttgttcgaga gcttgaacgc cgcaccgaag ctcgccgtga gatcggcgtc gatgtcgtcg
     2041 tcggtctcgt tcgtcagcga caccggcagc ctgatcgagt cacctgacgt gacctcgacc
     2101 ggcaaccgcg cgtcgagtgc gagcggcatc ttcgactgga tcaccgcttc gccaccgccc
     2161 ggcacgccgc ccgctgacac gccctccgcg atcgcacgga acgacgtcac cgcatccgat
     2221 accgggaacg acacggtcgc cgtgccatcg ctgctcgtgt cgacactcgc gttccagtag
     2281 atcgtctcgc ggaagtcggt gcgcggacct tcgtagccct tcttgtactc cggcaccggg
     2341 aacacgcgga ccggtgacca gccagcgacg agaccatcgt caccctcgcc gccccactcc
     2401 tcgtccttgt ccatcgccat gcgcgcgcgg ccgagcgcct gcttctcgcc cgccttgttg
     2461 tcccagcgcg cttccttctt cgcttgttcg ttgacggcct tccgatcggc gagcttgaga
     2521 ttcttcgcgc cgccgccggc cgcaccttcg gcgaggccta cgttgttgcc cttcggcttc
     2581 atcgcgactt tcgccctgcg ctcgggttgc ggcgccggct ctgcggccgc aaggtccatc
     2641 ggcgcttcga cagcaggcgg cacggcctgc acgaccgctg cacttcccca gcccgcaccg
     2701 ggtgcgatcg ccgcgctcgt cgcccacgtg tcgacaggcg gcggcgggtt gagcaccggc
     2761 tgccactcga acttgcggta gccgcgcgtc gcgagcagcg catccatcgc ggcgactgct
     2821 tccggcttat cgccgaagta gaagttcggc tcctcgatcg gatcctcgtc ggtcgcgccg
     2881 agctcgggct cgagatagag cttcgacaac atgcggccgc tcttgtcgtc cgcaaacgac
     2941 agcaccgtgt cgtcgaccac cgcgacgcct acgttcgcct tcaccggctt gcccgtcgga
     3001 tccgtcgtct tgatcttcaa cttcaccgga tcgcgcggcg agtaccgctt cttgtcggcg
     3061 gtgagctcga ccttcaggtc ctgccccttg ccgtgataga cgagacgctc ggcgagcggc
     3121 tcttgcttcg cggagaacag cgtcacgcgc gtcgcgcctt ggacgccctt gacatcgtcc
     3181 ttgatcggca gctcgatcag cgccggcttt cctgcctgca cctcgatcgc gccgtcggcg
     3241 agcttcttct cgcgcaggac ggcctcgacc atcacggtgc gattgctcga gcacagcgcg
     3301 gcgacgcgca gcttcgtcgg cgaatcgtca acgctgcgca cgacgcaacc gcccgacttc
     3361 gccgccggca cgtcgaactt ctgcgtgatg ccggccggct tcgtgatctc gacgtggtac
     3421 gtgcggtcgg catcgggcgc gagctcgaag cgaccgaggc catcgtggat cgacgcgaac
     3481 gtcgtcaccg tctgaccgcg atcgtcgacg atcttgccct cgatgtcggc cggcttgccg
     3541 atcgtgttct tcgccatgaa gtacacgcga cctgcgacgc cttcgacgag atcgccaccc
     3601 tcggggaaca gcgacagctg catcgtcttc atcacgatcg ggatgcgttt ctggatcgac
     3661 tcggtcacgc caccgtcgtc ggcgaggatc gtgagcaaac cgtcgccgcg cgcgatcttc
     3721 gccggcagct ggaacttcgc cgtcgcgccg ccgctcgcat ccgtcttgat cgcgacgcgc
     3781 gcgacctcga tgtcgtcgac cgtcacgacc gcggtcagcg ccttgtcggc aaacgcctcg
     3841 ccggtcgcgc gcttgatctc gatcgcagcg ctgacctgat cgccttcacc atacgccttg
     3901 cgcaggagct cgaggctctt cttcaagcgc ggcgcctcgt acgtgttgac gatgatcttc
     3961 ttcgtcgtgt gcgtgccgtc gtcggcggtg agctggatcg tgtactcgcc accctcgatg
     4021 tcgggcgaca gtgcgaagtc gttctgcgcg acgccgctct tcgcgagcac gcgtttctgc
     4081 gcgacgatcg atccgcgcgg cgacatcagc atcatgttga ggcccatgtc ggtcttgccg
     4141 accagcgaac cggtcgcgcg cagatcggcg cggaaccaga ttgtctcgcc cggctgatac
     4201 agtggcttgt ccgtcatcac gtagcttcgc tgcgatggat tgcgctggaa gtagtcgttg
     4261 attagcgacg acatcttcac gagcttgtcg ctcgcgaaca ccttcgccgg cgtcatcgcc
     4321 gcgatcgact tgtccagctg cgattgcgag gcagcctgct gcggaaccgc cgcaacatcg
     4381 ttcgccggcg gccccgaacc agcgcatgag gtggacagca gggcggcacc gaggaacaga
     4441 cggagtttgg tcatggctcg tccgtctaac gtgccgtcgc gaaaaacgat ctaggcgcgt
     4501 cgcgtcgtgg tcgagacacc gggtccgcgg aagtcttggg tcgcaaccga tgataccgtg
     4561 gcagtgatga tggccgccgc catcggcgcg cttgccgcga cgttagtgat cgcgctgagc
     4621 tggcgtaatg cgtgggcgcc tgccgtcggc gcactcctcg gcgttgccat cgcgttcgtc
     4681 gggggcgccg ccagcactgc cgacgtcgag cacgccgcac gcgacctgtg gcggccgctg
     4741 ctcgtgatca tcagcatcat gacgaccgcg gcgtgcgcga gcgagctcgg cgtctttgca
     4801 taccttgccg agtggatcga gccgcgtacg cgaggtccgg tacgccacgc gtttcgcctc
     4861 gtgttcgtgc tcgctgcgct gtgcgccgcc gtgctgtcga acgatgccgc gatcctgatg
     4921 ttcacgccgg tcgtgatcga gctgctccgt caggtctatc cgaagcgtca cggcaagttc
     4981 gtgcatgcgt tcgcgttcgc cgtgttcgta gccgctggcg tcgcgccgct ggcaacgggc
     5041 aatccgatga accttgtcgt cgcgcagcgc gccggcatcg gattcaacga gtacgcgcga
     5101 cacatgatcc cggtcgcgct cgtcggttgg atcgtcgcgt acctcgcgct cgcctggtac
     5161 ttccgcgccg agctcgccga cgaagcgccc gcgaaggggg gccgcttgcc gacggtgcag
     5221 ccggtcgacg gtcccgcgcg catcgtgctg ttcgcgacgg ccggcagcat tctcgcgtat
     5281 ccgatccttg ctgcgctcca ccagccgctg tggaccgttg cggtcccgac cgcgatcatc
     5341 tgcacgatgg cggcgactcg acgcgacgtg ccagtcactc gcatcttgcg cgaaatcagc
     5401 tgggagctga tcccgttcgt gttcggtgtc ctcgtgctcg cgaccgcgct cgcgcgcgta
     5461 ggtgttaccg actcgctcgc acaaatctac gctcggtcat ctgccccgtt acccacggtc
     5521 ggtgcggtcg ccgccgttgg ctccgcgctc atcaacaacc atccaatggc gctgctccac
     5581 tcggtggcac ttgcgggcga gccccactcg ctcgtctacg ccgcgttgat cggcggcgac
     5641 ctcggtcccc ggctgctgcc gatcggctcg ctcgccggtc tgctctggct ccataccctc
     5701 cgggtgcgag gcgtggtgat cccgttcggc ctgttcatcc gggttggcct cgtggtcacg
     5761 attccgtcgc tgctcgtatc gctggcgacc ctctggctgc tggcgtaacc accggaatag
     5821 gcaggcctgc gcccgcggtt gagggagttg catacatgtc ttccgtgcta caaacgatgc
     5881 ggaactgaga aggggtaaac ccatggcatt cgctcaagag tcagctcgtc cgattcaagg
     5941 cgccgtcgcc acgctaggcg tgagcgaccg cgtcgcattc ttgcgtaagt cgtatggcct
     6001 gctcggcggc gcgctgatcg tgtgggccgc ggcgtcggcc tacgtgttcc ggtacatgcc
     6061 ggaggtcagc ttcaagtggt cgcgctgggc gctcactggc tacaactggt tcgccgtgat
     6121 cggccttctg atcggcagcg gcatgctcgc gcagtggctc gcccgctccg agacgtcgcg
     6181 cacgatccag tacatcggcc tcggcgtcga ggttgctgca tggacattcc tgatgcagcc
     6241 gatgctgtgg gtgctgttca tgaagttcaa gccggctggt gcccaggcgc tgctcgcgca
     6301 aggcacgatc gcgacgctcg cgatcttcgt cggcctgacg gcgacggtct tcatcacgaa
     6361 gaaggacttc tcgttcctgc gcggcgtcgt gaccgtcggc atgttcgcag cgctcggcat
     6421 catcatggcg tcgatcctgt tcggcttcac gctcggcctg gtgttcaccg gcgccctgat
     6481 cgcgctgctc gcgctcaaga tcctctacga caccagcctg atgatgaact acttcccgcc
     6541 gacgcattac gtgtcggcag cactcatgct gttcggcact gtcgccaccc tattctggaa
     6601 catcatggtg ttcctcatga ggatgcgcag cgactaacga ttgcggctag gctgccgatt
     6661 tggcaggggt agcgacgggg accgaagagg tccccgttgt tcgtttcggg ctataatggg
     6721 ctgaatgtct gctgtggtgg cgccagcccc gctctcgtcg actgatatcg gcgcggtgcg
     6781 gcgactcgaa gatgcagtcg cgaccgtcat ccgcggcaag cccgaagcga ttcgcgccgc
     6841 ggtcatcgcg ctcttgtcgc gcggacatct attgatcgaa gacatcccgg gcgtcggcaa
     6901 gaccacgctc gcgcgcgcgc tcgctgccgc actcggcggc tcgttccgcc gcattcagtt
     6961 cacgagcgac ttgctcccct cggacatcgt cggcgtctcc gtctacgacc agcagggcaa
     7021 gatcttcgag ctcaagaagg ggccgatctt cgcgaacgtc gtgctcgcgg acgagatcaa
     7081 ccgcaccacg ccgcgcacgc aatccgcgct gctcgaggcg atgagcgaag gccaggtctc
     7141 gatcgacgac gcgacgcacg agctgccgcg cccgttcatg gtcatcgcga cgcagaaccc
     7201 ggccgagcac tacggtacgt atccgctgcc cgagtcgcag atggatcgct tcctgttgcg
     7261 cgtctcgctc ggctatccga acaagcacat cgagcgcgac ttgctcaaga accgccacgg
     7321 cgccgatccg gtcgccgagc tccagcccgt cgtcgatctg tcggccgttc gcacgctgca
     7381 ggacggcgtc gagacgatcc gcatggacga cgccctgctc gactacgcga tgcagatcgt
     7441 cgaggagacg cgccgtcatc cgtcgatcag cgtcggaatc tcgacgcgcg gcgcactcgc
     7501 ctggtaccgc gccgcgcaag ctgctgcgct cgccggcggc cgcgagttcg tcattcccga
     7561 cgacatcaag ggtctcgcta ttccgtgtct cgcgcaccga ctcgtgctcg cgcaggcgca
     7621 cgattcgctc ggtcgcgcgc gcgcagatag cgagcgcgtg atcgccgata tcatcggccg
     7681 cgtcagcgtt ccgacctgat gtcgttctgg gcgcgcctac gacgtcgctt tcgtccgccg
     7741 cgccggctga gcttcacccg cgaaggcaag ctcatcgtga tcctgtcggt cggcgttggc
     7801 ttcgccgcga tcaacaccgg caacaacctc ttgtacctgc tgctcggctg gctgctgtcg
     7861 ttcatcgtcg cctccgggat cctcagcgag ctgacgctgc gtgcgctcac cgtcgagcgc
     7921 aggccaccgc cgcgcatcta cgcgagcgag ccgttcttga tggaagtgtt gatcaagaac
     7981 ggcaagccga accgcgcgtc gtactcgatc gaggtcgaag acctgcaggg caagacgccg
     8041 atcgacaagc gctgctactt cctgaaaatc cccgaggcaa aatcgcagcg cacgtcgtat
     8101 cggcacacgt tcgtcaagcg cggcctttac acgctcaccg gctatcgact cgcgacgaag
     8161 tttccattcg cgctgtttcg caagtcacgc gacgtcgagg cgcagctcga cgtgctcgtc
     8221 tatcccgcgc gcgtctcggt accgcgcccg ccgccacgcg ccgcgacccg cggtgatgcg
     8281 atcgccgatc gactcggtcg ccgcggtgaa ttcttcggcc tgcgcgagcg ccgtcccggc
     8341 gacgatcgcc gcgacgtgca ctggaagtcg tccgcgcgca ccggccgctt gctcgtgcgc
     8401 gagtacgagg acgagctcgc ccgccgcgtc gtgatcggcg tcgacaacgc gctgccgcag
     8461 gacgtgcgcg acgcggtcac cgatggtgtg ctgacgccgg cgttcgaggc gcaggtctcg
     8521 gccgtcgagc gcgcgatcag catcgcggcg agcctggcga ccgcgtacct cgaggtcggc
     8581 tggaccgtcg agctcgtgtc gcgcggcgtg cacgtcgcgc ccgggaccgg ccgcatgcac
     8641 gaggcgaaga tcgcgcgcga gctggcgctg ttgccctacg tcggtgatga cgtcgcgttc
     8701 gcaacgatgc ccgctcgcat cgagagcgtg ctcgtccagc cgcgtggcgt cccggcgcaa
     8761 ggtcgcccgt cggcgggaac ggtgatggag gcctgatgcg cttcgccgtc gcccacaagg
     8821 ccgcgacgta tctgatgatc acgttcgcgt tcctcgcgat gatcgcgggt ggcggcgtgt
     8881 caccactgtt cgcgctcggt ggcatcgtcg gtctcgtcgg ctcgtggtgg tgggagccac
     8941 caaacattcg gtgggagaag tggacgtggg tatggacgac cgcatcggta ttcgcgctcg
     9001 tctacagcgt gctcaccgcg atcgtcaccg gtgatttcct cggcgtcggc gcgcagttct
     9061 tgatctggct cgtcgtcgcg aaggcgtaca accggcgcgc cgcacgcgac tggcagcaga
     9121 tgtacctgct cgcgttcctc atgctcgtcg cgggctcggt gctcaacgcc gatctcacct
     9181 acggtctgtg cttcctcggc ttcgtgatct cggcgacctg ggcactcacg ctgttccatc
     9241 tgcgccgcga gatggaagac aacctgctcg tcaagcatgc cgccgatcgc gcgagcgagc
     9301 gcgtcgaagt tcggcgcatc ctcgatagca agcgcatcgt cggcggccgc ttcttcctcg
     9361 gtaccggcct gctctcgttc ggcgtgttcc tcgtcgccgc gatcgtgttc ctcgcgatcc
     9421 cgcgcgtcgg cttcgggttc ttcctgaagg ggcgcggcgg gctcacgctc accgggttct
     9481 ccgacggtgt gaagctcggc ggtcacggcg tgatcaagaa cgactcgacc gtcgtgatgc
     9541 gcgtcgagat cgattcgaag tacggcggtc gcgacaaagc agagattcac tggcgcggcg
     9601 tcgcattcga ccactacgaa aatgcccgct ggacgcggac gcggctcgcg ccgtcgacac
     9661 gcgtcgtccg ctacatcgat cggccgggca tcgagcggta cctgctgagc tggcaggaca
     9721 gcgcggtcgc gttcgacgcg attcacgacg gcgcgaagca ggacatctgg ctcgatccgc
     9781 tcgactccga cgtgctgttc ggcgcgagca acccgcacgt gttcgagatg gcggcgatcg
     9841 cgcgcaagcg caaagagaag ctgcagctca acgacgagct gcgcgtcgat cacggctcga
     9901 cggtgcacta cacggtgtgg tccgacgtca cgacgccctc tcccgacgag ctgcgccgtc
     9961 cgatcggcgc tgcgcccgga aactacgcgg tgtatctgca gctgccgccc gagatcacgc
    10021 cgcaaacgtt cgcgctcgcg cgccggctca ccgacggcct accgacgaac tacgacaagg
    10081 cgatcgcgat cgagcgctgg ctcgagacga acctgtcgta cacgctcgag ctgaaggatc
    10141 cgggcaagac cgagccggtc cacttcttcc tgttcgatcg caagaagggc cactgcgagt
    10201 acttcgcatc cgcattcgtc gtactcgcgc gcgcagccgg catcccgacc cgccaggtca
    10261 acggcttcct cggcggcgag tggaacgagt atcagggcta cgtcgcggtg cgtgccggcg
    10321 acgcgcactc gtgggccgag gtgttttttc ccggtcgcgg ctgggtcacg ttcgatccga
    10381 cccctgcggc gcccggtggc gagctcggcc gcggtggcac cggctggcgc gcaaagctcg
    10441 gccgcttcat cgacacgctg cggttccagt ggtcgaagtg ggtgatcgag tacgacctcg
    10501 tcgcgcagct gtcgctattc aaggacatcg gccgcgcgct caagagtgcg gccggccacg
    10561 tacgcgatgg cgtcctcgcg gtgttccggt actggccgat cgcagcaatc ctcggcggtg
    10621 gaatcgccgg gctactcgta ctgcggcgac gacgccgtgg ccctggcatg gaacacggcg
    10681 cgcgcgtggt gaagccgcgt acgcgatcgc cgatcgcggc ggtctacgat caagtgacga
    10741 agcagctcgc caagcacggc acccggcgcg agcccgggac cacgccgcgc gagctggccg
    10801 accgtgccct cgcgaagggc gatccggcgg cgaccgagct ccgggagctg accgagctgt
    10861 attacgccgc cgagtggggt ggccgacgcg atccgggcgc cgagcagcgg gccggagagc
    10921 tggcgatcgc gatccggcgc cggctgcgcg actcggcaaa atccgcgacc tgaacgtaga
    10981 gccgaccgcg cgaagtccgc gatctgatcg actgaccgcg tgacgtggcc cacgacaccg
    11041 gcgtcccacg ggacgcgcca gccgcatggt gctcgccatc accgcttcgc gttgacggct
    11101 cggcttacta ttcggctggc ttgtgccaga ttggcagtcg gtccggcgaa attgaaacgc
    11161 cgatctgccc gtgatatcga cgaatgtcgt cggtccacct tcgcacgggc gacggcaaac
    11221 cccttgcaga gagcagccgc gatgacgcgt tacgtactgc tggccttcct cggtctgggg
    11281 gcgagcatct cgtgggccga tatgggcatg ctgcccgacg gcgcgcagct caagttcaac
    11341 aaggtgctcc tgcacgagca caacgccgaa gtgccggccg agcccgagat cgatcccgac
    11401 tcgctgctgt tctatttcaa ctcggcgcac tgcgagtgca gcttcttcaa ctacaacccc
    11461 gagacggcgg acatgacgtt cttcgagaac aacgtcgcgt tcgagctgac cgtcgagggc
    11521 ggcagtacgc cggtcactga cccgatcgag atctggaccg gcaccggctg cgacacgaac
    11581 gacacgacgc agcgcgcggc cgactgccac aaggtcgact cggccggtgc cggcaccgtt
    11641 gctgcgatcc agcagtccgg cgccgcgacc atctcggtcc cgatcttcga cgtcatggta
    11701 ccgaaggcga gcgaacgccc cggctgcaag gagcgcgagc aggacggtac cgtgtgggcg
    11761 ctcgtcgacg tcgggggcac cggcatgacc aattactcgg tatcgaagac gctaaagacc
    11821 gacacgaagc cgccgccact accgacgaac ttccacgcgg tcggcgccga gaacgcggtc
    11881 gtgattactt gggactcgcc ggctgaaggc gtctccgacg tcgcgtacta ccaggcgctg
    11941 tgtgcgaccg ccggtggcgc gccggcaaaa tcgaacccgc cgaaggcgcg ttatcagaca
    12001 tcacgccagc tgtgcggtgc gccatccgac gtgttcctca cgccgtcgga tatctcgtcg
    12061 aacaccggga ccgacgcagg ctcgggctcg atcgtgctca ccgaggagat ggcgcagctc
    12121 cagcccgcgt tcatctgcgg cgagagcgcg agcgcgacca aaaacgagct gcgcatcgac
    12181 ggcctcaaga acggtagcga atacacggtc atcttgctca cgatcgatcg gtcgggaaac
    12241 gcgacgggca cgttcttcac gtcgccgctc gtgccgcagc ccgcgaccga cttctgggag
    12301 gacctccacg atcaaggtag cggcgtcgag ggcggcttct gcttgctcgc gcaaacgttc
    12361 ggcgatgaca acccgctcac gaacacgctg cgttcgttcc gcgacaacac gctcgccgac
    12421 accgcgtatg gccgctggtt gatcgatgtc tactacggca cgatcggcgc gctcgatctg
    12481 cacggcagcg tcgcgctgcg catcgtcgcc ggtgtgttgc tactgccgct cgtcgtgctc
    12541 gcgctgctct ggcatctgct cacactgccc gggctactcg cgctcgccgc gctgctcgtg
    12601 ctcatccgga agcgtcggtt cgcacaggcg cgcctcgccg ccgtgaccac cgtggcgttc
    12661 gtcgcgttgt tcccggcgcg cgcacacgcg caatcgccgt actgggagac gcaggagtac
    12721 acgaacaccg aggccgagct gccgcccggt gatccgatgc gcgtgcgctg gcacgcaggc
    12781 attcgcctcg gtccgtacgt gcccggcatc gacgaccagg tgaacatgcc ggtcggcaag
    12841 ttcgcgggcc cgtacgagca gatgttcggc ggctcgtcgg tcctgcccgt gctcgacgtc
    12901 gactacttct tgtggcgcgg cttcggccag ctcggcgttg gcgcatcgct cggctacctc
    12961 ggcaagaagg cgcacgcgtg gcaggccggc agcgatccgg cggacccgaa gcgacctcgc
    13021 gcggaaggcg acgaaaacaa gttccgactg ttcccgttct cgctgaacgt ggtctatcgc
    13081 ttcacgtacc tcgacgacga atacggcgta ccgatcgtgc cctacgcgcg cggcggcctc
    13141 gcctactaca tctggtggat caccgcaccg aacggcgact tctcgaagtc gtgcgtcgga
    13201 cctaacactg gcgcgatgtg ccagacgacg acggcggcag gcgccagcct cggcgtcgtt
    13261 ggctcggtcg gcctggcggt tcgcgccgag cgcgtcgacg agggcgcggc ccgatcgatg
    13321 cgcgaaagcg gcatcgagca cgcggggttc tacgccgaat acagtattgg caaagtcgac
    13381 ggcttcggct cggataagaa actctccgtc ggcgacgcca cttggttcgc cggcgtggac
    13441 ttcgagttct aggctcgcgc taaacgcgac gcgtcgacgg ccgcgggctg cgcaatccgc
    13501 gcgacgcgct gccagcaact gacacgtaga gcgttgtgtc ccaggctgcg tcgccatgtt
    13561 atcgacgagc caaatgaagc gcgtgttggt ccctgccctg ctgctcgctg cctgtggcgg
    13621 cgccaaaccc gaagccgttg tccccgcacc gccgacgagc agcgcagccg cgccggcagt
    13681 cgccgcggcc gagcctgccg cggtcacgtc gacgaagtcg gctccggcgc gaaagatccg
    13741 cacggtcgag ggcatcaccg agtaccagct cgcgaacgga ctccaggtgt tgctgttccc
    13801 cgacgcgacg cagtcgacgg tgacggtcaa cgtcacgtac ctcgtcggct cgcgccacga
    13861 aggttatggc gagaccggca tggcgcacct cctcgagcac atgatgttca agggcacgcc
    13921 gacgcatcgc aacgtactca agctgctcgg cgagaagggc gcgagtgcga acggcacgac
    13981 ctggctcgat cgcacgaact acttcgagac gctgccggcg tcgcaggaga acctcgactg
    14041 gacgctcgcg ctcgaagccg atcgcatgcg caatgcatcg atctcgccgg acgatctcaa
    14101 gaccgagttc tcggtcgtcc gcaacgagtt cgagatgggc gagaacaacc cgcaggcgat
    14161 tctcgacgag cgcatcgtgt cgactgcatt cctctggcac aactacggca agtcgacgat
    14221 cggctcgcgc tccgacatcg agcgcgtgcc ggtgccggcg ctgcgcgcgt tctacgagaa
    14281 gtactaccag cccgacaacg cggtgctcgt cgtctcgggc aagtacgacg acgcgaaggc
    14341 actcgcatcg gtcgagaaga tgttcggttc gattccgcgc ccgacgcgcg tgctcgcgcc
    14401 gacgtattcg gtcgagcccg cgcaggacgg cgagcgcacc gtgacgttgc gccgcaacgg
    14461 cgacgtcaac gtgatcggtc tcgcatatca cacggtcgct ggtacctcgg acgacttccc
    14521 ggccgtcgaa gccgcgctcg acatgctcac gcgcgagccg gcgggccgcc tgtacaagaa
    14581 gctcgtcgag acgaagctcg ccgcatcgat cgatagcacg gcgatggcga cgcacgatcc
    14641 attcctcgcg cagctgtccg cggacgtccg cgacgcgaag aacgtcgcca ccgtcgagaa
    14701 gatcatgatc gaggagatgg aaggcctcgc cgcgtcgaag atcgacgaca aggccgtcgc
    14761 gcgctggcgc gtggcgacgc tcaaggagct ggagctcgcg atggccgata gcgagcgcat
    14821 cgccgtcgag ctttcggagt acgcagcgct cggcgattgg cggacgctgt tcgcgtatcg
    14881 caaccgggtc gagaaggtga cggtcgccga cgtccagcgc gtcgcgaaga cgtacttcaa
    14941 ggcgtcgaac cgcacgctcg gcaagttcat cccgacgaag gacatcgatc gcgcgccgct
    15001 caccgagacg ccggacatca acgcctacgt gaagggcatc gagggcggcg aggtaaaaga
    15061 gcaaggcgag gtgttccagg cgacgctcga caacatcgag cagcgcacga cgcgcacgca
    15121 gctcaagggc ggcatcaaag cggcgctgct gccgaagaag acgcgcggcg gcaaggtcga
    15181 gctgaacctc gcgctgcact ggggtgacga gaagtcgctg cagaacaagt cgacgcaagc
    15241 cgacatgctc ggctcggtgc tcgtgcgcgg cacgacgaag aagtcgtacc aggacctcca
    15301 ggatctcgag gatcagctca aggctcacat ctggttctcg acgagcgccg acggcctcac
    15361 gctgcacatc gagacgctgc gcgacaagct gcccgccgcg ctcgatctcg cggtcgagat
    15421 gctcaagacg ccgagcttcc ccgacaagga gctcgagatc gtgcgccagg agcagctctc
    15481 gtcgctcgag caacagctgc aggagccgat ggcggtcgcg ttccagacgg tccagcagct
    15541 gacgacgaaa tggccgaagg gcgacccgcg ctatcccgaa tcgacgaccg aacagatcgc
    15601 cgcgacgaag aaggtcacga tggcggagat caagcagttc tatcgcgact tcgtcggcgc
    15661 cggttacggc gagctgtccg tcgtcggtga cttcgatccg aaggcgctca ccgcgcagat
    15721 cgagcaacac ttcgagaagt ggcagaccaa gaagccgtac aagcggctcg agggtaaggc
    15781 attcggcgtc ccgggcgcga cgaagtcggt cgacatcaag gacaaggaga tgacgacgct
    15841 cgcgctgtca cacgacatcc agatgaagga caccgacgcc gactacgcgg cgtggctgat
    15901 ggcgtcgcag atcctcggcg gcgacgcagc gtctcgtgcg tggatgcggc tgcgcgagaa
    15961 ggaaggtctg tcgtacggcg ttgccacgtg ggcgttcgcc gatgcgttcg acaacgtcgg
    16021 cggctttggc ggctacgcga tcgtcgcgcc gcagaacctc gcgaaggcga aggcgtcgct
    16081 gctcgacgag atcgaaaagc tgacgacgag caaggtcacc gacgaggagc tccggcgcgc
    16141 gaaggagacc tggatcaagg agcaggacac cggcctgtcg agcgacggct acgtgatcca
    16201 gatgctgtcg cgccagacgt tccgtggccg cacgacggcg tacacgaagg agctgcgcgc
    16261 gaagatccag gcggtcaccg ccgacgatgt cgcgcgggtc gcgaagaagt atctcgatcc
    16321 gaagcggctc gtcatcgtcg acgccggcga cgtctctaag cagcagaaga agtagcgata
    16381 cttcagcgcc gctgcgagcg cggagctaat cggccttctt cggcacttcg gcgaggtacg
    16441 tgtcttcctc ggcctcgtcg gtgcgaatcg tcgcgcccgg ctcggcgggc acgcccgata
    16501 acaacaagct gatctgtgcg ccgagcgtct gcgcgcttgg ataccggcga tcgggattct
    16561 tttcgagcat gcacgcgatc agggactcga catcggctgg cacgtccgca cgcaccgtcc
    16621 gcaacagcgg cgcgggcgcg tgaatgtgtg ccgcaaccat ctcgccgaac gtgcgcgact
    16681 ccgcgaacgg atgctgcccg gtgatcaaca agaatgcgag cacaccgagc ccgtagatat
    16741 cgctgcgcgc gtcgagccca cgatcgagca attgctccgg cgagctgtac tcgaccgagc
    16801 cgacgttctg gccgaccggc gagatgcgca ggttcgcgcc aaggatcttc gcgaggccga
    16861 agtccgacac cttcacgaag tacgcatccg acggccgttc ctcgacgatg atgttctcgg
    16921 gcttgagatc gcgatgcacg acgcccatcg cgtgcgcttc gccgagcgag ttgcacagcc
    16981 cgagcaggat ccgcatcacg gtttgccacg gcagcggacc gtcgtgccgg agcacgtgcg
    17041 cgaggctgcg gccgggcgac agctccatcg cgatgtacaa ggagccgtcg ggctcgcgat
    17101 cgaattcgta cgtcgtgatc gtgtgctgcg agcgcaggtt gacgagcacg tggccctcgc
    17161 ggcgaaagcg ctcggtcgcg agtggatcgt gttgcgcgtt gcccggcagc agcttgatcg
    17221 cgatcttgcg gcgggttccg gtctgctcgg ccttgtagac cgcgcccagc gagccggcgc
    17281 cgacccgctg ccgcagctgg taacgccgcg cgatcgtgcg gccgaagttc tcgtcgacga
    17341 ggctcacgcc gcagacgcca caaaagcgcg cctcgtcctc gaggtcctca tcgcaggtgt
    17401 ggcaccgggt gctcacgcga ggcacagcat agctcgggac cggggtgccg ggtgggtcgg
    17461 gcgtttcgtg cttcaagcgt cgacggtgga tgggctactc tgacggtatg aggacgacgg
    17521 tcgtgtgtct cgtggtgctc ctcttcgcct cgccggttcg agcagactcg ccgtcgcgcg
    17581 gccacgtggt cgtcgatgcg ctcggcgtcg atgcgcgaac cggtgagaag ctcatcgcga
    17641 tcgtcgatgc gcacgatgcc gagctggcga agctttcgcg cgagcgcggc gagctcaaga
    17701 gccagctggt cgcatcccgc cggctcgatc ccaaatcgct cgatggcctg ctcgacgcga
    17761 ccgtcgcgaa ccagcgcgcg ctcgccgagc tcgacggccg gttgatccag cgcgtgcgta
    17821 agctcgtgcc gagccccaag gcagttcagc tgttgttcct gctgtcggtc accgagccga
    17881 cacttcctgc agcaaccgtg agcactgccg tgtgcgaccc gtggacatcg atgcatcgtt
    17941 gcaactgaca gtgacggatc ccacccaatc ccgttagatc acgaaacact caatttgccc
    18001 tcggggctcg gtgggcgtgt tagggtgcgc gccctcgatg cggcccctga tcctcgtctg
    18061 tgttgcgttt ggtctgatca cttctgcggg atgcggtggg tgtgatcgcg
//
LOCUS       DASUFX010000561         5974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_367928,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000561 DASUFX010000000
VERSION     DASUFX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367928"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFV99_22540"
     CDS             <1..864
                     /locus_tag="VFV99_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062173.1"
                     /translation="LPDAELYKPYVLDGTTFYAGDASWQWTVTSDNPCDAVFLTTTTK
                     GPTYDLAGGVTSTITLTPRLTGDYTLHAMVTPTSGPAFQCDMVVHAGLPGLRVEQCSD
                     KSSTADLDLHLHRPGTTTNWETTPDDCYYMNCKADAVAIANWGYANSPIAACQDSPTG
                     TQWQTVGSCRNPRLEVDSIMNDGVPEIAVVDTPIDSATYRVAVRYYQGTVTAHPIVTI
                     NCGGKRAAAYGVAPDQVTGFNQSGPTGQIWRVADVTTTVTNGVTDCTVTPLHPPGQIS
                     GYWVQSNSLAY"
     gene            complement(861..1472)
                     /locus_tag="VFV99_22545"
     CDS             complement(861..1472)
                     /locus_tag="VFV99_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062174.1"
                     /translation="MMTLAGKPATPALLCITGASGAGKTAALCALREKIEARVLPTLA
                     FDSLGVPSEGEMQSAWDSPRGWQKAMTYHWVYTAKHVYRTHPLVVLEGSFDPQYAIAA
                     CAAHRVRSLVVLLHTDDGVRSDRLGKRGQPELATAEMTSWASYLQEQTQQLGGVVVDA
                     SPAIDHVVDAICTHALPLLEPRDEAPRDARERIRAKRGSRPDN"
     gene            complement(1527..3500)
                     /gene="tkt"
                     /locus_tag="VFV99_22550"
     CDS             complement(1527..3500)
                     /gene="tkt"
                     /locus_tag="VFV99_22550"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5062175.1"
                     /translation="MTTDLQTLAINTIRTLAIDAIQKANSGHPGLPMGCAPMAYTLWQ
                     RHLKHDPRSPQWFDRDRFVLSAGHGSMLLYSLLHLTGYDLSLDELKNFRQWGSKTPGH
                     PEWHHTPGVEATTGPLGQGAANAVGMALAERYLARYFNRAGHSIIDHYTYALVSDGDV
                     MEGVAMEAASIAGHLGLGKLIYLYDANQITLDGPLSLSMSEDVGERYAACGWHVQHVE
                     NGDKDVNAIDAAIQAAKLEGDRPSIIIIRTTIGYGSPKKAGTASAHGSPLGDAEVAAT
                     KQALGWDPNAKFLVPDEVRAHLSEGQRRGAAAHAAWREKLSAYKAAHPDLGTQLELAI
                     DGKLPAGWDEGLPTFSGATATRNASGKIMNALATKIPWLLGGDADLGGSTKTIVAGGD
                     YDRTGAGRNLRFGIREHAMTAIGNGMLYHGGVRSFVSTFFVFSDYMRPSVRLAALNKQ
                     PAIFVWTHDSIGLGEDGPTHQPVEHLMALRAMPNLTLFRPCDGNETVVGWKLALSRTH
                     SPTGLVLSRQDLPTVTQTGAPGAEKGAYVLADGKDAIIIASGSEVAIALAARDELAKG
                     GVAARVVSMPSWELFAEQDQSYRDSVLPPSQYKRVSVEAGATFGWRQYVGDRGESVGV
                     DTFGASAPGEVLYEKYGLTPAAVVAAVKRVVAQ"
     gene            3540..5324
                     /locus_tag="VFV99_22555"
     CDS             3540..5324
                     /locus_tag="VFV99_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062176.1"
                     /translation="MRSVKALVLLGICACGGGKPASSTPPTPPTPAPAPGPTYPNGTP
                     TAAVTPAPPEKPFAVEDKSEPEWVPPAWKKVAVGQTIAISTAAIDQDLDETSVVVNKM
                     PPSAKFDPITQTIVWTAAKADMPKASFEITIAQPGRNKTATRSFDIQVVQGKAVAAPA
                     ASEQTPVIETLLMIRQPGRLAQVNKDWPLDKLLQVGAETFKLQIPEDKRAKLKTPLDG
                     ATSYKQMLQGLAQTHQNPRLDPESPQFDKAFASPSDWKIVAVRPRIDKAWTELRVVYQ
                     AVKAPEPVFAMFRLRPVVEYVPALPRPDEERDANNKIFLGMVAKHLMKNGSPNPKFVK
                     DQVAHGKAVSALMTELMTFDDTKTKPYLHGFEIGIALEARMGGGSARNSDGSYKSGDA
                     WGWSAQKPFQTSEGSTQVYQNVAIPGFWTQTVPSKDGKTWVPRCAPRFNPEDKFVPGY
                     EILCRKTMGFVDLPDTKDGKIVNGRRDANNQFFEHKKRFMVDGFALEDGRRDLGEENG
                     MTCSQCHIRNFGMHDYSDSANVDPSKGVPKAPNKALATLNFQIIPTDRWEAFTIDFLH
                     HQECRGKMFLEQYLGADSAKGLTCPLAK"
     gene            5324..>5974
                     /locus_tag="VFV99_22560"
     CDS             5324..>5974
                     /locus_tag="VFV99_22560"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5062177.1"
                     /translation="MAEPKKKLPVSDSKAAKTRAGGWQAFTDGGTASGWVIAARVVMV
                     LALAATIPTILDIEHGNRLLWTVCIAALPFFWMTVGYHVWRRICPLAVAGQLGRIVGK
                     PGVRKMGDWMSKHYLIVQLGLMIAALSLRLVLTNGSAIGLAVFLGAVVFAAIGVSFVY
                     GGKTWCNFLCPVGLVEKIYTEPVRSSSGVVEDMTSQCSPCVACKKHCPDIDLEQGYW"
BASE COUNT         1052 a         1985 c         1969 g          968 t
ORIGIN      
        1 ctgcccgacg ccgagctcta caagccctac gtgctcgacg gcaccacgtt ctacgcgggt
       61 gatgcatcgt ggcagtggac cgtgacgagc gataacccat gcgacgcggt gtttctcacc
      121 accacgacga aggggcccac gtacgacctc gcgggcgggg tgacatcgac gatcacgctc
      181 acgcctcgcc tgaccggcga ctacacgctc cacgcgatgg tcacgccgac gagcggaccg
      241 gcgtttcagt gcgacatggt cgtgcacgca ggtttgccgg ggctgcgcgt cgagcagtgc
      301 tcggacaaga gcagcactgc cgacctcgat ctccatctgc atcgacccgg taccaccacc
      361 aactgggaaa ccacgcccga cgattgctac tacatgaact gcaaagcgga cgcggtggcg
      421 atcgcaaatt ggggctacgc aaactcgccg atcgccgcat gccaggactc gccgaccgga
      481 acccagtggc agaccgtcgg ttcctgtcgc aacccgcgcc tcgaggtcga tagcatcatg
      541 aacgatggcg tcccggagat cgccgtcgtc gacactccga tcgacagcgc gacgtatcgc
      601 gtggcggtgc gttactacca aggaacagtc accgcgcatc cgatcgtgac catcaactgc
      661 ggcggcaagc gcgccgcggc gtatggcgtc gcgcctgacc aggtcaccgg gttcaatcag
      721 agcggtccca ccgggcagat ctggcgcgtc gctgacgtga cgaccacggt gacgaacggt
      781 gtcaccgact gcacggttac gccactccat ccccccggcc agatcagcgg ctactgggtg
      841 cagagcaatt cgctcgcgta ctaattgtct ggccgcgagc cgcgcttcgc ccgtattcgc
      901 tcgcgcgcat cgcgaggcgc ttcgtcgcgc ggctcgagca gcggcagcgc gtgcgtacag
      961 atcgcgtcga cgacgtgatc gatcgcgggt gacgcatcga ccacgacgcc accgagctgc
     1021 tgcgtctgct cttgcaggta gctcgcccag ctcgtcatct ccgctgttgc gagctcgggt
     1081 tggccgcgct tgccgagccg gtcggagcgc acgccgtcgt cggtgtgcaa cagcacgacg
     1141 agcgaacgca ctcgatgcgc cgcgcacgct gcgatcgcgt actgcggatc gaacgagcct
     1201 tcgagcacga cgagcggatg cgtgcgatag acgtgcttcg ccgtgtacac ccagtgatac
     1261 gtcatcgcct tctgccagcc gcgcggcgaa tcccacgccg actgcatctc gccttcgctc
     1321 ggcacgccga ggctgtcgaa cgcgagcgtc ggcaacacgc gcgcctcgat cttctcgcgc
     1381 agcgcgcaca gcgcagccgt cttgcctgcg cccgacgcgc ctgtgatgca gagcagcgcc
     1441 ggtgtcgccg gcttgccggc caacgtcatc actcgctcgc gtaacgtcgc caccgacatc
     1501 gcggtcgaga ctaccagacg cgtctgctat tgggcgacga cgcgcttgac cgcggcgaca
     1561 actgccgctg gcgtgaggcc gtacttctcg tacagcacct cgccgggcgc cgacgcgccg
     1621 aacgtgtcga cgccgaccga ctcgccacga tcaccgacgt actggcgcca gccgaacgtc
     1681 gcgcctgctt cgacggacac gcgcttgtat tgcgacggcg gcagcaccga atcgcgatag
     1741 ctctggtctt gctcggcgaa cagctcccac gacggcatcg acacgacgcg cgccgcgacg
     1801 ccacccttgg cgagctcgtc acgggcagcg agtgcgatcg cgacctctga gcccgacgcg
     1861 atgatgatcg cgtccttgcc gtcggcgagc acgtatgcgc ccttctcggc tcccggcgcg
     1921 ccggtctgcg tgaccgtcgg tagatcctgg cgcgacagca cgaggccggt cggcgagtgc
     1981 gtacgcgaga gcgcgagctt ccagccgacg accgtttcgt tgccgtcgca cggacggaac
     2041 agcgtgaggt tcggcatcgc gcgcagtgcc atcagatgct cgaccggctg gtgcgtcgga
     2101 ccgtcctcgc caaggccgat cgagtcgtgc gtccacacga agatcgccgg ctgcttgttg
     2161 agcgcggcga gccgcacgct cgggcgcatg tagtcgctga acacgaagaa cgtcgacacg
     2221 aacgagcgca cgccgccgtg atagagcatg ccgttgccga tcgcggtcat cgcgtgctcg
     2281 cggatgccga agcgcaggtt gcggccggcg cccgtgcgat cgtaatcgcc gcccgcgacg
     2341 atggtcttcg tcgagccacc gaggtcggcg tcgccgccga gcagccacgg aatcttcgtg
     2401 gcgagcgcgt tcatgatctt gccggaggcg ttgcgcgtgg ccgtcgcgcc ggagaacgtg
     2461 ggcagcccct cgtcccagcc ggcaggcagc ttgccgtcga tcgcgagctc gagctgggtg
     2521 ccgagatcgg gatgcgcggc cttgtaggcg ctgagcttct cgcgccacgc ggcatgcgcg
     2581 gcggcaccgc ggcgctggcc ttcggagagg tgcgcgcgaa cttcgtcggg gacgaggaac
     2641 ttcgcgtttg gatcccagcc gagcgcctgc ttggttgctg cgacctcggc atcgccgagc
     2701 ggcgagccgt gcgccgacgc ggtgcctgcc ttcttcggcg agccgtaacc gatcgtcgtg
     2761 cgaatgatga tgatcgacgg tcgatcgcct tcgagcttcg cggcctggat cgcagcgtcg
     2821 atcgcgttga cgtccttgtc gccgttctcg acgtgctgga cgtgccagcc acacgcggcg
     2881 tagcgctcgc cgacgtcctc gctcatcgac agcgagagcg gaccgtcgag cgtgatctgg
     2941 ttggcgtcgt agagatagat gagcttgccg agcccgaggt ggcccgcgat gctcgcggcc
     3001 tccatcgcga cgccttccat cacgtcgccg tcggagacga gtgcgtacgt gtaatggtcg
     3061 atgatcgagt ggcccgcgcg gttgaagtag cgagcgaggt agcgctcggc gagcgccatg
     3121 ccgaccgcgt tcgcagcgcc ttggccgagc ggaccggtgg tcgcctcgac acccggcgtg
     3181 tgatgccact cggggtggcc cggcgtcttg ctgccccact ggcggaagtt cttcagctcg
     3241 tcgagcgaca gatcgtagcc cgtgaggtga agcagcgagt agaggagcat cgagccgtga
     3301 ccggccgaca gcacgaagcg atcgcgatcg aaccactgcg gagaacgcgg atcgtgtttg
     3361 agatggcgct gccacagcgt gtacgccatc ggcgcgcaac ccatcggcaa gcccgggtgt
     3421 cccgagttcg ctttctggat ggcgtcgatc gccagcgttc gaatcgtgtt gatcgcgagc
     3481 gtctgaaggt cggtcgtcat cggcgagcgg accctagcat gatggtacga tgccgcggca
     3541 tgcggagcgt gaaagcccta gtcctcctcg gtatctgcgc gtgtggcggc ggtaagccgg
     3601 cgtcatcaac gccaccaacc ccgccgaccc ctgcgccggc gcccggtcca acctatccaa
     3661 acggcactcc gacggccgcg gtcactccgg cgccgcccga gaagccgttc gccgtcgagg
     3721 ataagagcga gcccgagtgg gttccgcccg cctggaagaa ggtcgcagta ggtcagacga
     3781 tcgcgatttc gaccgcggcc atcgatcagg atctcgacga gacgagcgtc gtcgtgaaca
     3841 agatgccgcc gagcgcgaag ttcgatccga tcacgcagac gatcgtgtgg acggccgcga
     3901 aggcagacat gccgaaggcg agcttcgaga tcacgatcgc acaacccggc cgcaacaaga
     3961 ccgcgacgcg ctcgttcgac atccaggtcg tccagggcaa ggccgttgcc gcgccggcgg
     4021 cgtccgagca aacgccggtg atcgagacgc ttctaatgat ccggcagccc gggcgcctcg
     4081 cgcaggtgaa caaggattgg ccgctcgaca agctcctgca ggtcggcgca gagacgttca
     4141 agctgcagat tcccgaggac aagcgcgcga agctgaagac gccgctcgac ggtgcgacgt
     4201 cctacaagca gatgctgcag ggtctcgcgc agacacatca gaacccgcgc ctcgatcctg
     4261 agtcgccgca gttcgacaag gcgttcgcga gcccgagcga ttggaagatc gtcgcggtgc
     4321 ggccgcgcat cgataaggcg tggaccgagc tgcgcgtcgt ctatcaagcg gtgaaggctc
     4381 ccgagccggt gttcgcgatg ttccgactgc gccccgtcgt cgagtacgtg cccgcgcttc
     4441 cgcggcccga cgaagagcgc gacgcgaaca acaagatctt cctcggcatg gtcgccaagc
     4501 acctgatgaa gaacggcagc ccaaacccga agttcgtcaa ggatcaggtc gcgcacggca
     4561 aggcggtgtc cgcgctgatg accgagctga tgacgttcga cgacacgaag acgaagccgt
     4621 acctgcatgg cttcgaaatc ggcatcgcgc tcgaggcgcg gatgggcggc ggctcggcgc
     4681 gcaacagcga cggcagctac aagagcggcg atgcgtgggg ctggagcgcg cagaagccgt
     4741 tccagaccag cgaaggctcg acgcaggtct atcagaacgt cgcgattccc ggcttctgga
     4801 cacagaccgt gccgtcgaag gacggcaaga cgtgggtgcc gcgctgcgcg cctcgcttca
     4861 accccgagga caagttcgtc cccggctacg agatcctctg ccgcaagacg atgggcttcg
     4921 tcgacctgcc cgacacgaag gatggcaaga tcgtcaacgg tcggcgcgac gcgaacaatc
     4981 agttcttcga gcacaagaag cgcttcatgg tggacggctt cgcgctcgag gacggccgtc
     5041 gcgacctcgg tgaagagaac gggatgacgt gctcgcagtg ccacattcgc aacttcggca
     5101 tgcacgacta cagcgattcg gcgaacgtcg atccgagcaa gggcgtgccg aaggcgccaa
     5161 acaaggcgct cgcgacgctg aacttccaga tcatcccgac cgatcgctgg gaagcgttca
     5221 cgatcgactt cctgcatcat caggagtgcc gcggaaagat gttcctcgag cagtacctcg
     5281 gggccgattc cgccaagggc ctgacctgcc cgctcgctaa atagtggccg aaccgaagaa
     5341 gaaacttccc gtctcagata gcaaggccgc gaagacgcga gccggcggct ggcaggcgtt
     5401 caccgatggc ggtacggcgt ccggctgggt gatcgcagcg cgcgtcgtga tggtgcttgc
     5461 gctcgccgca acgattccga cgatcctcga catcgagcac ggcaaccgcc tgttgtggac
     5521 ggtgtgcatc gccgcgctgc cgttcttctg gatgaccgtc ggctatcacg tatggcggcg
     5581 catctgcccg ctcgcggtcg ccggccagct cggccgcatc gtcggcaagc cgggcgtgcg
     5641 caagatgggc gactggatga gcaagcacta cttgatcgtc cagctcggcc tgatgatcgc
     5701 cgcgctgtcg ctgcgcctcg tgctcacgaa cggcagcgcg atcgggctcg cggtgtttct
     5761 cggtgcggtc gtattcgccg cgatcggcgt gtcgttcgtc tacggcggca agacgtggtg
     5821 caacttcctg tgccccgtcg gtctcgtcga aaagatctac accgagcccg tgcggtcctc
     5881 gagcggcgtc gtcgaggaca tgacctcgca atgctcgccg tgcgttgcgt gcaagaagca
     5941 ctgccccgac atcgatctgg agcagggcta ctgg
//
LOCUS       DASUFX010000562         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_89345,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000562 DASUFX010000000
VERSION     DASUFX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89345"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFV99_22565"
     CDS             complement(<1..753)
                     /locus_tag="VFV99_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062178.1"
                     /translation="MRITALALALLLPACIVGSGEIDGVGDDQTGGGGGEGGGGGGGG
                     GDGSGSGSGSDVMNTPHITASVDKTAVSTELGKTETIVLTINSVDSFVGAVSVTPSVL
                     EGTTPVTGWQVTANPPSVDLQADASAQVTLSVKIPTDTTSLMPDLKVDLGSTAAPVSV
                     ESAFTVANQLTINIPANTGTGAPHAGLPATNSPIRLRLGAKVIFHNGDTISHQIHAAG
                     GINHEGSALAAGADYTVTPSDNATWYCHLHETS"
     gene            complement(864..1145)
                     /locus_tag="VFV99_22570"
     CDS             complement(864..1145)
                     /locus_tag="VFV99_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062179.1"
                     /translation="MLPALIVGALTAWYLGLRIGAVVAVATAVALLVATFVPGMSITI
                     YALVIAWCAALYFFGPKISKASGKSSMSSMFGSPFAQAKAYMSKLWKSK"
     gene            1273..2688
                     /locus_tag="VFV99_22575"
     CDS             1273..2688
                     /locus_tag="VFV99_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5062180.1"
                     /translation="MQKLIVVLLLAAACTKPNPNRCCTDEADCTAKGIPVGSICEQGL
                     VCRGNQCISQPCASSTECDATAPYCVAELCAEACNDDAQCPGFGQAASDRFCVEGACA
                     ECRIGMNDCTTAAPVCDQGSCRQCRDHNECQSSVCSADGSCADESSIAYVETNGAANS
                     NCTRTSPCSTIARALMLARRYVLIGPGTYPSTAAIVLSGQVDLIGSGTRPILTRSTPG
                     PIVSISSGQIGVDNLQLSGATTNASDYGYGLYCEATTGAPVLKARRILARDNNLGGMY
                     AQSCSVEVTESTFTNNGRGTSGPGIKIVNGTGVIDRCTVSDNDTGIELDSGLFQVTNC
                     FIFHNDSPSTSYGINLYSTEGGQKIEFNTIVDNARSAVVGRGFNCQLDGVTGSFPNNL
                     FVRNKLQTAGANCTYPSSIVVDTDVTALKFNSPDVPPFDYHIGAGSMAIDAAAGSTVD
                     HDFDGQPRTAPRDVGADEFVP"
     gene            complement(2689..4197)
                     /locus_tag="VFV99_22580"
     CDS             complement(2689..4197)
                     /locus_tag="VFV99_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062181.1"
                     /translation="MPQVVELPPIGQDQRGALAALVRVCKDTPAKGESFREFRTRLRA
                     AKLWDRERPVVILRFLGVGGTQIVPSAFMQSVAAAMTDDDTAHAIMDRVWQLNPLLGK
                     TIVDLVAQRAYGKDEIYKVLVSSVFKGAIPSRPGLETWLQIAIGNGVLKPLGIAVVQG
                     ARFDRYAQLGAAFDVEEFLEEDKPEPDPFIPVAGEEEAAAAAPAAEVAVVSETSIPVF
                     AAAPTGSLLPAPLRHLSADGVASPRGRDRATPLSRFSSGFSDEVLGETSKKLAAWWTD
                     ANVTSQTYQPADFKLDAEQWVENADEVLYRIAVAAALAFRLDRDRAGVIAAYTALDKA
                     GVLADLYQGTVPENLPAHVDARALMLASLSARRCAEVPELAASLDGRGSAAEAFASLD
                     AALGRGLFRTELFWIFDMLSRLGVIRYDDLGDYTVTPHRIVRDTLFRLGFIESPYASD
                     GTALSAAAKAARRVVPTGPADEILTLFALSAGCSYDCAHRKTCDFPCRERLE"
BASE COUNT          797 a         1395 c         1438 g          727 t
ORIGIN      
        1 cgacgtctcg tgcaggtggc agtaccaggt cgcgttgtcg cttggcgtga cagtgtagtc
       61 ggcgccggca gcgagcgccg agccttcgtg attgatgccg ccggccgcat ggatctggtg
      121 cgagatggtg tcgccgttat ggaaaatcac tttcgctccg aggcggagac ggatcggcga
      181 gttggtcgcc ggcagcccag cgtgcggcgc gcccgtacca gtgttcgcgg ggatattgat
      241 cgtcaactgg ttcgccaccg tgaacgcgga ctcgacgctg accggtgccg cagtgctgcc
      301 gagatcgacc ttgaggtcgg gcatgagcga cgtggtgtcc gtcgggatct ttaccgacag
      361 cgtcacctgc gcgctggcgt cggcctgaag atcgacgctc ggcggattcg ccgtcacctg
      421 ccaaccggtg accggcgtgg tgccctcgag taccgacggc gtcacggata ccgcgccgac
      481 gaagctgtcg acgctgttga tggtgagaac gatcgtctcg gttttgccga gctcggtcga
      541 gaccgcggtc ttgtcgacgc tggcagtgat gtgcggcgtg ttcatcacgt cgctgccgct
      601 gcccgatccc gagccatcgc caccgccgcc acctccaccg ccgccttcac caccaccgcc
      661 accggtttgg tcatcgccaa caccatcgat ttcacccgag ccaacgatgc acgcggggag
      721 gagaagagcg agtgcaaggg cggtgatgcg catgtacgca cttgttacaa gggtcgtgcc
      781 gcgactaacc gcctgttatc cgtcgcgtcg cgagcggcaa accggggtga ctactccccc
      841 aggaagtgcc cacagtcccg tggctacttg ctcttccaga gcttcgacat gtacgccttc
      901 gcctgggcga acggtgaacc gaacatgctc gacatgctgc tctttccgga tgccttggag
      961 atcttcggcc cgaagaagta gagcgcagcg caccacgcga tcacgagcgc gtagatggtg
     1021 atgctcatcc cgggcacgaa cgtggcgacg agcagcgcga ccgcggtcgc aaccgcaaca
     1081 acagcgccga tacgcaggcc caggtaccac gccgttaacg ccccaacgat gagagcagga
     1141 agcatttgct ccaaaccgta gcacagggct gtcggttcgc tcacaaaccg gcccgaggac
     1201 agggtttacc tagctcgtcg ggcagggata tagacggggc aactcagcgg tcccgaggtc
     1261 gagaaggtgg tcatgcaaaa gctcatcgtt gtcctgttgc tcgcagctgc gtgcaccaag
     1321 cccaatccga atcgctgctg caccgacgaa gccgattgca cggcgaaagg catcccggtc
     1381 gggagcatct gcgagcaggg gctcgtgtgc cgcggcaatc aatgcatctc gcagccgtgc
     1441 gcgagctcga ccgagtgtga cgctaccgcg ccgtactgcg tggccgagct atgcgcggag
     1501 gcctgtaacg acgacgcgca atgccccggc tttggccaag cagcaagcga tcgcttctgc
     1561 gtcgaaggcg cgtgtgccga gtgccgcatc ggcatgaacg actgcacgac cgccgccccg
     1621 gtctgcgatc agggtagctg ccgtcaatgc cgcgaccaca acgagtgtca aagcagcgtc
     1681 tgctccgccg atggcagctg cgccgatgaa agctcgatcg cgtacgtcga gacgaatggc
     1741 gcagcgaact cgaactgcac gcgtacgtcg ccgtgcagca cgatcgcacg tgctctcatg
     1801 cttgctcgtc gttacgtgct aatcgggcct ggcacctacc cgagcacggc ggcgattgtg
     1861 ctctccgggc aggtcgatct gattggttcg ggcacgaggc caatactgac acggtcgacg
     1921 ccgggcccga tcgtctcgat aagcagcggg caaatcggcg tcgacaacct gcaattgagt
     1981 ggcgcgacta cgaacgccag tgactacggc tatgggctgt actgcgaagc gactacaggg
     2041 gctccggtac tgaaggccag gcgcatcctc gcccgcgaca acaaccttgg cggcatgtac
     2101 gcgcagtcct gctcggtcga ggttaccgag agcacattta cgaacaacgg gcgtggcacg
     2161 agcggtccgg gcatcaagat cgtaaacggt accggtgtca tcgatcgctg tacggtcagc
     2221 gacaacgaca cggggatcga gctcgactcg ggccttttcc aggttacgaa ctgcttcatc
     2281 ttccacaatg actcaccgag cacgagctac ggcatcaacc tctactcgac ggagggaggg
     2341 caaaagatcg agttcaacac gatcgtcgac aacgccagga gcgctgtcgt aggtcgtggg
     2401 ttcaattgcc aactcgacgg cgtcaccggt tcgttcccca acaacctctt cgtgcgaaac
     2461 aaactgcaga ccgccggcgc gaactgtacg tatccaagct cgatcgttgt cgacaccgat
     2521 gtcacagcgc tgaagttcaa ctcgcccgat gtcccgccgt tcgactacca catcggcgcg
     2581 ggcagcatgg cgatcgatgc cgctgcaggt tcgacggtcg accacgactt cgacggccag
     2641 ccgcgcacgg caccgcgcga cgtcggcgcc gacgaattcg tcccgtagtt actcgaggcg
     2701 ctcgcggcac gggaagtcgc acgtcttgcg gtgcgcgcag tcgtacgagc agcccgcgct
     2761 gagcgcgaac agcgtgagga tctcgtcggc gggccccgtc ggaacgacgc gacgcgcagc
     2821 cttcgccgct gccgacagcg ccgtgccgtc actcgcgtac ggcgactcga tgaagccgag
     2881 ccggaacagc gtgtcgcgca cgatccgatg cggcgtgacc gtgtagtcgc cgaggtcgtc
     2941 gtagcggatc acgccgaggc gcgacagcat gtcgaagatc cagaacagct cggtgcggaa
     3001 cagacctcgg ccgagcgcgg catcgagcga tgcaaacgct tcggcggcag agccgcggcc
     3061 atcgaggctc gcagcgagct ccggaacttc agcgcaacga cgcgcggaca gcgacgcgag
     3121 catcagcgcg cgggcgtcga cgtgggccgg aaggttttcg gggacggtgc cctggtagag
     3181 gtcggcgagc acgccggctt tgtcgagcgc ggtgtacgcg gcgatcacgc ccgcgcgatc
     3241 gcgatcgaga cggaacgcga gcgccgcggc gacggcgatg cgatagagca cttcgtcggc
     3301 gttctcgacc cactgctcgg catcgagctt gaaatctgcg ggctggtagg tctgcgacgt
     3361 gacgttcgcg tcggtccacc acgcggcgag cttcttcgat gtttcgccga gtacctcgtc
     3421 ggagaagccg gatgaaaagc gcgagagcgg cgttgcgcgg tcacggccgc gcggcgacgc
     3481 gacgccgtcg gcagagagat ggcggagcgg cgcgggcagc agtgagccgg tcggtgcagc
     3541 agcgaagact ggaatcgatg tctcgctcac cacggcgact tcggcggccg gcgcggcggc
     3601 ggctgcttcc tcttcacctg cgaccggaat gaacggatcg ggctcgggct tgtcctcttc
     3661 gaggaactct tcgacatcga acgccgcgcc gagctgcgcg tagcgatcga agcgcgcgcc
     3721 ctgcactacc gcgatgccga gcggcttgag cacgccgttt ccgatcgcga tctgcagcca
     3781 cgtctcgagg cctggtcgcg acggaatcgc gcccttgaag acggaggaga cgagcacctt
     3841 gtagatctcg tccttgccgt aggcgcgctg ggcgacgagg tcgacgatcg tcttgccgag
     3901 cagcgggttg agctgccaca cgcggtccat gatcgcgtgc gcggtgtcgt cgtcggtcat
     3961 tgcggccgcg accgactgca tgaacgcgga cggcacgatc tgagtgccac cgacgccgag
     4021 gaatcggagg atcacgaccg ggcgttcgcg atcccacagc ttcgcggcac gcaagcgcgt
     4081 gcggaactct ctgaacgatt cgcccttcgc cggcgtgtct ttgcaaacgc gcaccagcgc
     4141 cgccagcgca ccacgctggt cctggccaat cggcgggagc tccacgacct gaggcatcgc
     4201 tcgcgaaggt aacacgcacg cgttgcacgt cggcgggcgg tcggatgcga gttgctcgga
     4261 tcgccgccgc gtgttgtgct ggtcggggtg ggattcctcg acaagctcgc gatcgtcgcc
     4321 ggcaacttcg ccgcaccggg gttcgcgcaa gcgctgt
//
LOCUS       DASUFX010000563        11998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_89463,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000563 DASUFX010000000
VERSION     DASUFX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89463"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VFV99_22585"
     CDS             <1..1356
                     /locus_tag="VFV99_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062182.1"
                     /translation="GLATHLFYSVNSGDPATGNPFVLYGGLQDNGTRFRASPTTPSAF
                     NQAIGGDGIGGTVHTGQDGTTYWASIQFQWAFCKPALTDCSVEQPEAQTDAESHWHGA
                     PSPVGLEPEQIQERAMERLARFGEDSEPFLVHYSNVETDKIGQSVLTHTDEQVFVAVP
                     AGDGFTWQSISQDLTNSAVGAGISNVTASRSTPGLYGAAGLVSAEPFYVTTQGNTKVN
                     WTVAQPVRIGSARLTGPSSIDFPPTLEAGQVPGQVFIGAFTGTLNNQTRSPPPDDQGH
                     LYRTTDGGQTWKSIVGADPAHRLPNVPVYVVRYDPIEAKTIYAGTDLGVYITLDGGAT
                     WDRMGDGLPMVPVRDVYIAQNQDFIRVATYGRGLWEIYPSAGAGHGAPGNGDYDRNLQ
                     IDWIDLGAMSARQGVTPEQSTPPLYSWIMDMTGVGSSPPVQAIDAEDLAVFLTKFGGH
                     P"
     gene            1353..1925
                     /locus_tag="VFV99_22590"
     CDS             1353..1925
                     /locus_tag="VFV99_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062183.1"
                     /translation="MIRSLLLLSVLVAACGDNQDAPLHYSNPAAGGKLRLVKNTTLQT
                     TDTEVALDLVVGDQPLTGYSVGFNLPVGHRLVHLVDFIPGTALDPGSAPAAAQAALPI
                     AGPLADTLATGLSQKATGPGAVTTDTTVAPKSVLYTIRLQMVPNAADGVAFDGTAADF
                     HLPSGGMRDRGGTTVVEANEVAIGKLEINR"
     gene            1922..2635
                     /locus_tag="VFV99_22595"
     CDS             1922..2635
                     /locus_tag="VFV99_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062184.1"
                     /translation="MKRSVVTLAVLGSFAGIAHAERARELATSAAFTPGILRATDTGN
                     GFVNVASDFDGSTSSVRVSALGEVNLWWRVRGAVRLLDAFSDKPRPGVGAGLRWLDGE
                     TASTAYLFYKTEGFTEPEGEVEALLSFERAFGAVRASANIAYGQDPEGNERDGELAFI
                     AHVEPRTGWFIGGTARYRDALGSTKEAVIRDGFAGPTSTLTLGAFAISLNAGVAMAET
                     QMTARKFGPASTLSLGAAF"
     gene            2637..2936
                     /locus_tag="VFV99_22600"
     CDS             2637..2936
                     /locus_tag="VFV99_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062185.1"
                     /translation="MHVTQLIYVRPGSEAAFEQFESVVLPLLARYRGELVLRLRLRPD
                     NQIGGTAETPYELHIARFATAADLEAYSNDPERQRWLHLKDQAVERAIMLSSPDG"
     gene            3001..3735
                     /locus_tag="VFV99_22605"
     CDS             3001..3735
                     /locus_tag="VFV99_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062186.1"
                     /translation="MTELRCADLDAAVAEHAARGFRIDMIMPADDPETIEMSCGDTRV
                     RLLRDAPTRTIDGHWHQGRAGMEYRDLIPDRRGGRLIASHIRIRDGGPVPDYVHYHAI
                     TFQLIYVRAGWVRVVYEDQGPPFVMHAGDCVVQPPLIRHRVLEASPGLEVIELASPAR
                     HATYADHELQLPAPSVRPDRDFSGQRFSWHRAAATRAPVEDLGVAAATGGLVTARILR
                     DGAAASGRISIPVDDGVILDVAMHGD"
     gene            complement(3746..4141)
                     /locus_tag="VFV99_22610"
     CDS             complement(3746..4141)
                     /locus_tag="VFV99_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062187.1"
                     /translation="MNSRLTIDGAMNRGVFLAKLWALLGPAASRTGGFEYYVHDQETN
                     LAFVAYVGPRGPAYGGDPEHRVALRRVVEAFEDVLEQTQAVDCTIEYQADPEYGGGTW
                     VLGYKDGRSFDLPDRRNRPPSGQIERRVR"
     gene            4360..5289
                     /locus_tag="VFV99_22615"
     CDS             4360..5289
                     /locus_tag="VFV99_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5062188.1"
                     /translation="MQSPIVTITNLSKTYDSGLRALDNVSLSIQRGEILALLGPNGAG
                     KTTLIGIVCGIVRPTGGTVTADGHDIQGDYRAARAKIGLVPQELSTDAFETVWATVTF
                     SRGLFGKRANPAYIEKVLRALSLWDKKDSKIMTLSGGMKRRVLIAKALAHEPSILFLD
                     EPTAGVDVQLRHEMWQLVRELRNTGVTIILTTHYIEEAQEMADRIGIIDKGKLVLIED
                     KATLMRKLGKRELTLQLMRPLERIPDALAAQPLELSSDGTTLTYRFNIQSEQTGIAPL
                     LSELANLGIELKDLQTKESSLEDIFVSLVKGAA"
     gene            5286..6047
                     /locus_tag="VFV99_22620"
     CDS             5286..6047
                     /locus_tag="VFV99_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5062189.1"
                     /translation="MNIYAIRAIYRFEMARTFRTLMQSILAPVLSTSLYFIVFGSAIG
                     SRMGTVGGVDYGSFIIPGLVMLSLLGESISNASFGIYLPKWSGTIYELLSAPVSFVEV
                     VIGYVGAAATKSIILGTLILSTARVFVPYEIEHPVWMITFLLLTSVTFSMFGFIIGLW
                     ADSFQRLQVIPLMVITPLTFLGGAFYSINMLPPLWQKITLFNPVVYLISGMRWSFYGM
                     SDVNVAISVTATTGFLLACLAVIAWVFKTGYKLRT"
     gene            complement(6051..6848)
                     /locus_tag="VFV99_22625"
     CDS             complement(6051..6848)
                     /locus_tag="VFV99_22625"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5062190.1"
                     /translation="MPASIPFGLGICIYLPTLLWAPAPAHAPAPHTNAVSIAAAVPAP
                     SLTPEVAAAPASTVHVVLTRGQKRLDLDLPLDGKVAPETADAIALMMRDPNSGRTKRI
                     ASGTLALLADVAVRYPGHEIEVVSAVRAEPERSRDGIKHSKHWDGHAIDLIVKGAKLS
                     EVRDVMWKNHRDVGIGWYPEGGFIHLDYRPDVHDMAWTQPKKNADNIYHPRWSRLARD
                     PEAEAAASRAEMRRVLAKHPTLSRFVSSLAKVMGYETPAVTHDRSSS"
     gene            7016..8317
                     /locus_tag="VFV99_22630"
     CDS             7016..8317
                     /locus_tag="VFV99_22630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5062191.1"
                     /translation="MASKPTTNDETLTLVVSTPRAAAAAWLVVGAGTPDERRIEIGDK
                     PLCIGSDPAAEVCVADPHVSRRHAEIVGGSGNVVIRDLQSRNGTYVDGIAVKEAILQE
                     RATIRIGGTTIRFETEAPTSAAGPRRMGEAVGSSPEMQRVFEMLERLAPSDLTITLLG
                     ETGVGKDVLAGAVHDASARRDGPFIVFDCGAAAPTLIESTLFGHEKGSFTGANATVVG
                     AFERAHGGTLFFDEIGELSLDLQPKLLRALEQRRIQRIGSTDEIPVDVRILAATNRDL
                     EAEVAAGRFRQDLFFRLSAAVVAVPPLRARLADLPELVDAILATEGLRLRVTADTLEA
                     LATYDWPGNVRELRNVLTAAAAMADADLLEPKHLLFFKQRRRNATLADFPLGGRSLES
                     IERAAIVQTLKAADGNKVKAAKALGIAASTLYEKMRRYRIA"
     gene            8433..9422
                     /locus_tag="VFV99_22635"
     CDS             8433..9422
                     /locus_tag="VFV99_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062192.1"
                     /translation="MRIPASTLALVTCLAVSGSADAKPHRHGRHHKVKKAKHRKVAPP
                     VERVRETDDGESDRDRVAIAMPDADDETIEFSDDDVDADRDDRDERDDRDEAPVTLRK
                     TAVSRTKDWHVAIGPYLWASSVDANVSLGSANVAAGVDFIEVTRHARYGAEVLGEARY
                     RRFSLAGDVMYGVVALTGEREVGPLMVTLGGQASSLLVDGTAGYLVTGDDHALLSLEA
                     RGGVRYQRTEVAGSVGIDGNPVQSTSVVDAGGDAVAGARAVLRPWNRFYVSGAADVGV
                     YGVSNSTWSASVDASVRIGSHVLVSLGWRTLTIDRATIAVTMHGPRGAVQLVF"
     gene            9575..11185
                     /locus_tag="VFV99_22640"
     CDS             9575..11185
                     /locus_tag="VFV99_22640"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062193.1"
                     /translation="MRITCWGSVVFGIVAAACVGPLPPPPELTVTSPQRGLVQGEAGQ
                     IVVKGTALPGPSGDPVARVTVNNTPALLAADGSFSAVVNVPAGAMLLETVAVSDAGAK
                     ATDARAVQVGQLRPVGTSIDRAVTAAVSADAFAKLSAAAGPIIKSTNLMALIAQVQPK
                     LWDDIANVQLTITKLAFADVKISLAPVDGGLQFSAKIDGLSVAANAAYGGVLVPDGTT
                     SVSATADTVTIAGTLVVTPAGTAGFTTSIASPLVGTTNLRLQASGLVGQILDLLNNNL
                     ASTIQRITTSTAEKAMAPLITKAFGALAGPQQLDVLGKKLEIQASPSAVQFSHTGALV
                     TLNLQVKIAGSESSPGFIYTPNGMPTMNVGTGVQLGLADDLINELLAEVHALQLLNIH
                     LDEDFGLFDAADFKLTLPPMISANNSDGTLRLVLGDMIATFTDHGNPVIRAAVNAQID
                     LAILRGTSAQEIALQFGKVHAYVNVLDNAESSDDLSGPTKDGIGVQLDRLSKFMITVP
                     VPSVAGVSLDNLALRADSGYVVVSGQIH"
     gene            11318..>11998
                     /locus_tag="VFV99_22645"
     CDS             11318..>11998
                     /locus_tag="VFV99_22645"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX5062194.1"
                     /translation="MRGAVWMLLYLFFILAPLFALLLGAHPPARSFWTEFSIAIGYAG
                     LAIMGLQFALTARFRYVTAPWGEDVIYHFHRRVSLLAVGLVIAHPIILFIERPELLAL
                     LNSFQAPWRARMAALSTYSLIALVAMALWRTKLRLSYETWHVTHVVLAVVAVAAGVAH
                     MVGWSFYFEDPWKRGLWLGLTVFWIGLLGYVRIIKPLFVIRRPYRISAVRKERGNTWT
                     LVMKPEGHA"
BASE COUNT         2001 a         3943 c         3943 g         2111 t
ORIGIN      
        1 ggcctcgcga cccatctctt ctattcggtg aactcgggcg atccggcgac cggtaatccg
       61 ttcgtgctct atggcggctt gcaggacaac ggcacgcgat tccgcgcgag cccgacgaca
      121 ccgtcggcat tcaaccaggc gatcggtggc gacggcatcg gcggcaccgt gcacaccgga
      181 caagacggca cgacgtactg ggcgagcatc cagttccagt gggcgttctg caaaccggcg
      241 ctgaccgact gttcggtgga gcaacccgaa gcgcagaccg atgccgagtc gcactggcac
      301 ggcgcaccgt cgccggtcgg tctcgagccc gagcagatcc aggagcgcgc gatggagcgc
      361 cttgcccggt tcggcgagga ttcggaaccg ttcctcgtcc actactcgaa cgtcgagacc
      421 gacaagatcg gccagagcgt gctgacgcac accgacgagc aagtgttcgt tgcggtgccg
      481 gcgggtgatg gtttcacgtg gcagtcgatc tcgcaggatc tgacgaacag cgcggtcggc
      541 gccggcatct cgaacgtgac tgcctcgcgc tcgacgcccg gcctctacgg cgccgctggt
      601 ctcgtgtcgg cagagccgtt ctacgtgacg acgcaaggca acacgaaggt caactggacg
      661 gtcgcgcagc cggttcgcat cggctcggcg cgtctcaccg gcccatcgtc gatcgatttc
      721 ccgccgacgc tggaggcggg acaagtgcca ggccaggtgt tcatcggcgc gttcaccggc
      781 acgctgaaca accagacacg ttcgccgcca cccgacgatc agggtcacct ctatcgcacg
      841 accgacggtg gtcagacgtg gaagtcgatc gtcggtgccg atccggcgca ccggctgccg
      901 aatgttccgg tctacgtcgt tcgctacgat ccgatcgaag cgaagacgat ctacgcgggc
      961 accgacctcg gcgtgtacat cacgctcgac ggcggcgcga cctgggatcg catgggcgac
     1021 ggtctgccga tggtgccggt gcgcgacgtg tacatcgcgc agaaccagga cttcatccgc
     1081 gtcgcgacct acggccgcgg gctgtgggag atctatccga gcgcgggtgc aggtcacggc
     1141 gcgcccggca atggcgacta cgatcgcaat ctgcagatcg actggatcga tctcggcgcg
     1201 atgtctgcgc gccagggcgt gacgcccgag caatccacac cgccgctcta ctcctggatc
     1261 atggacatga ccggggtggg cagcagcccg ccggtccagg cgatcgatgc cgaggacctc
     1321 gccgtgttcc tgacgaagtt cggaggtcac ccgtgatccg ctcgctcttg ctcctttccg
     1381 tcctcgtcgc tgcgtgcggc gataaccagg acgcaccgct ccactactcg aatcccgcgg
     1441 ccggcggcaa gctgcgtctg gtcaagaaca ctacgttgca gacaacggat accgaggtcg
     1501 cgctcgatct cgtcgttggc gaccaaccac tgaccggtta ctcggtcggc ttcaatcttc
     1561 cggtcggcca tcggctcgtg cacctcgtcg acttcattcc gggcaccgcc ctcgatccag
     1621 gaagtgcacc cgcggcggcg caagctgcgc tgccgatcgc ggggccgctc gcggacacgc
     1681 tcgctacggg actctcgcag aaggcgacgg ggccgggtgc ggtcacgacg gacaccacgg
     1741 tcgcgccgaa gtccgtgctc tacacgatcc ggttgcagat ggtgcccaat gcagccgacg
     1801 gcgtcgcgtt cgacggcacg gccgccgatt tccacctgcc atcgggcggc atgcgtgatc
     1861 gcggtggtac taccgtcgtc gaagcgaacg aagtggccat cggaaagctg gagatcaatc
     1921 gatgaagcga tcagttgtta cgctcgccgt ccttggctcg ttcgcaggca tcgcccatgc
     1981 cgagcgcgcg cgcgagctcg ccacgagcgc cgcgttcacg ccgggcatct tgcgcgcgac
     2041 cgataccggc aacggcttcg tcaacgttgc ctcggacttc gacggttcga cgtcgtcggt
     2101 tcgcgtctcg gcgctcggcg aggtgaatct gtggtggcgc gtgcgcggcg ccgtgcgctt
     2161 gctcgacgca ttctcggaca agccgcgccc gggcgtcggc gcaggtctgc gctggctcga
     2221 cggcgagacc gcgtcgaccg cgtatctgtt ctacaagacc gaaggcttca ccgaacccga
     2281 gggcgaggtc gaagcgctgc tgtcgttcga gcgcgcgttc ggcgcggtgc gtgcgagcgc
     2341 gaacattgcg tacggccagg atcccgaggg caacgaacgg gacggcgagc tcgcgttcat
     2401 cgcgcacgtc gagccacgca cgggctggtt catcggtggc accgcgcgtt atcgcgacgc
     2461 gctcggcagc acgaaggaag ccgtgattcg ggacggcttc gccggcccga cgagcacgct
     2521 cacgctcggc gcattcgcga tcagcttgaa cgcaggcgtc gcgatggccg agacgcagat
     2581 gacggcccgc aagttcggcc cggcgagcac gctgtcactc ggcgccgcgt tctgacgtgc
     2641 acgtcacgca actgatctac gtgcggcctg gcagcgaagc cgccttcgag cagttcgaga
     2701 gcgtcgtgct tccgctcctc gcgaggtatc gcggcgagct cgtgctgcgg ctgcggctcc
     2761 gacccgacaa ccagatcggc ggcacggccg agaccccgta cgagctgcac atcgcgcggt
     2821 tcgcgaccgc ggccgacctg gaggcgtatt cgaacgatcc cgagcgccag cgctggcttc
     2881 acctcaagga tcaggcggtg gaacgcgcca tcatgctgtc atcgcctgac ggctgagtgc
     2941 cacgttcgtt tcaatccgtt tcgtcggcac cgctcgctcg cgactgagta cagtctgcac
     3001 atgaccgagc tgcgctgcgc cgatctcgat gctgcggtcg cagagcatgc agcacgcggc
     3061 tttcgcatcg acatgatcat gcccgccgac gatcccgaaa cgatcgagat gtcctgcggc
     3121 gatacgcgcg tgcgtctact gcgcgacgca ccgacgcgca ccatcgacgg acattggcac
     3181 caaggccgcg ccggcatgga gtaccgcgat ctcatcccgg atcgccgtgg cggtcgcctg
     3241 atcgcgtcgc acattcgcat tcgcgacggc ggtcctgtac cggactacgt ccactaccac
     3301 gcgatcacgt ttcagctcat ctatgtccgc gccggttggg tacgcgtggt ctacgaagac
     3361 caagggccgc cattcgtcat gcatgccggc gattgcgtcg tgcagccgcc gctcattcgc
     3421 caccgcgtgc tcgaagcttc gccgggtctc gaggtcatcg agcttgcctc tccagcgcgg
     3481 cacgcgacgt acgccgatca cgagctgcag ctgccggcgc cgagcgtgcg gccagaccgg
     3541 gacttcagcg gccagcgctt ttcctggcac cgcgccgccg ccacgcgcgc gcccgtcgaa
     3601 gatcttggcg tcgctgccgc aaccggcgga ctcgtgacag cacgcatcct gcgtgacggc
     3661 gccgccgcat cgggccgcat ttccatcccc gtcgacgatg gcgtgatcct cgacgtcgcg
     3721 atgcacggcg actagcgcag atcgtttaac ggacgcgtcg ctcgatctga ccgctcggtg
     3781 gccgattgcg acgatccggt agatcgaacg accgaccatc tttgtaaccg agcacccagg
     3841 tgccgccgcc gtactcgggg tccgcttgat attcgatcgt gcagtcgacg gcctgcgttt
     3901 gttcgaggac atcttcgaac gcttcgacga cgcgccgcag tgcgacgcgg tgctccggat
     3961 cgcccccgta cgcgggtccg cgcggcccga cataggcaac aaacgcgagg ttcgtctcct
     4021 gatcgtgcac gtaatactcg aaaccgccgg tgcgcgacgc agccggtccc agcagcgccc
     4081 acagcttcgc gaggaagacg ccgcgattca tcgcgccgtc gatcgtcaac cgcgaattca
     4141 cgtcacgacg gctaaacagt ttggatccca agagctgcac cgatgcccgt ctgaatcgca
     4201 tgcttgggag agcgccgaca tgcggtcagg tcgcacgaaa acgacaggca cgtcggtgct
     4261 ggaacacagc gtacagcgcc cctacctgat ctaggaggtg atcggccgcg cacgcgggtg
     4321 tcggatcggc cacgcttcgt tcgtctcctt ggagcagtca tgcagtcccc gatcgtcacg
     4381 atcacgaacc tgagcaagac gtacgacagc ggcctccgcg cgctcgacaa cgtcagcctc
     4441 tctatccagc gcggcgagat cctcgcgttg ctcggcccga acggcgcggg caagacgaca
     4501 ctgatcggca tcgtctgcgg catcgtgcgg ccgaccggcg gcacggtcac cgccgacggt
     4561 cacgacatcc agggcgacta ccgcgcggcg cgggcgaaga tcgggctcgt tccacaagag
     4621 ctgtcgaccg atgcgttcga gacggtgtgg gcgacggtga cgttctcgcg cgggctgttc
     4681 ggcaagcgcg cgaacccggc atacatcgag aaggtgctgc gcgcgctgtc gctctgggac
     4741 aagaaggact cgaagatcat gacgctgtcg ggcggcatga aacgccgcgt gctgatcgca
     4801 aaggcgctcg cgcacgaacc ttcgatcttg ttcctcgacg agcctaccgc gggcgtcgat
     4861 gtccagttgc gccacgagat gtggcagctc gtgcgcgagc ttcgtaatac cggcgtcacc
     4921 atcatcttga cgacgcacta catcgaagag gcgcaagaga tggccgatcg gatcggcatc
     4981 atcgataagg gcaagctggt cctcatcgaa gacaaggcga cactgatgcg caagctcggc
     5041 aagcgcgagc tgacattgca gctcatgcgg ccactcgagc ggattcccga tgcgctcgca
     5101 gcgcagccgc tcgagctatc gtccgacggc acgacgctca cgtatcgttt caacattcag
     5161 agcgagcaga ccggcatcgc accactcctc agcgagctgg caaacctcgg catcgagctc
     5221 aaagatctgc agacgaaaga gagctcgctc gaggacatct tcgtcagcct cgtgaagggg
     5281 gcggcgtgaa catctacgcg attcgcgcga tctatcgctt cgagatggcg cgcacgttcc
     5341 ggacgctgat gcagagcatc ctcgcgccgg tgctgtcgac gtcgctctac ttcatcgtgt
     5401 tcggctcggc gatcgggtcg cgcatgggca cggtgggcgg cgtggactac ggctcgttca
     5461 tcattccggg gctcgtcatg ctgtcgctgc tcggcgagag catctcgaac gcgtcgttcg
     5521 gcatctacct gccgaagtgg tcgggcacga tctacgagct gttgtcggcg ccggtatcgt
     5581 tcgtcgaagt cgtgatcggc tacgtcggtg ccgccgcgac gaagtcgatc atcctcggca
     5641 cgctgatcct cagcacggcc cgcgtgttcg tgccgtacga gatcgagcat ccggtctgga
     5701 tgatcacgtt cctgttgctg acgtccgtca cgttcagcat gttcggtttc atcatcgggc
     5761 tgtgggccga tagcttccaa cggctccaag taatcccgct gatggtgatc acaccgctga
     5821 cgttcctcgg cggcgccttt tactcgatca acatgctgcc gccgctgtgg cagaagatca
     5881 cgctgttcaa tcccgtcgtg tatctcatca gcggcatgcg ctggtcgttc tacgggatgt
     5941 cggacgtgaa tgtcgcgatc agcgtcacgg cgacgaccgg gtttcttctc gcgtgcctcg
     6001 cggtgatcgc gtgggtcttc aagacgggct acaagctgcg gacgtagcgc ttacgagctg
     6061 ctgcggtcgt gggtgacggc cggcgtttcg tagcccatca ccttcgcgag cgacgacacg
     6121 aaccgcgaca gcgtcggatg cttcgcgagc acgcgacgca tctccgcgcg cgatgctgcg
     6181 gcctcggcct caggatctcg tgcgaggcgc gaccaccgcg gatggtagat gttgtcggcg
     6241 ttcttcttgg gctgggtcca cgccatgtca tggacgtccg gacggtagtc gaggtgaatg
     6301 aagccaccct ccgggtacca gccgatgccg acgtcgcgat ggttcttcca catcacgtcg
     6361 cgaacctcgg agagcttcgc gcccttcacg atgagatcga tcgcgtggcc atcccagtgc
     6421 ttcgagtgct tgatgccgtc acggctgcgc tcgggctcgg cgcgcaccgc ggacacgacc
     6481 tcgatctcgt gaccggggta gcgcacagcg acgtcagcaa gtagtgcgag cgttccgctc
     6541 gcgatgcgct tcgtgcggcc actgttcggg tcgcgcatca tgagcgcgat cgcatctgcg
     6601 gtctccggtg caaccttgcc gtcgagcgga aggtcgagat cgagccgctt ctggccgcgc
     6661 gtcagcacga cgtgaaccgt ggatgcgggt gcggcagcca cttcgggcgt gagcgacggc
     6721 gcgggcacgg ccgcggcaat gctgaccgcg ttcgtgtgcg gggcaggagc gtgggccggt
     6781 gccggagccc acaggagcgt ggggaggtag atacaaatcc cgagaccgaa cggaatgctg
     6841 gcgggcatgg cacctcctag ttacaagggg tgtgccgcct gcaggtgctt gaaatctcga
     6901 aggctggccg cccggccggt ctccgaaaat cggagaccct gaccgaattg cgtgcaggtc
     6961 acgaatcctc cggattgacc cggacttatg cgcgcaacta cgatgatcgc gtcgcgtggc
     7021 ctccaaaccg acgaccaacg acgagacgct gacccttgtc gtatcgacac cgcgggccgc
     7081 ggctgccgca tggcttgtgg ttggcgccgg cacgcccgac gaacggcgga tcgagatcgg
     7141 cgacaaaccg ctctgcatcg gatccgatcc ggccgccgaa gtgtgcgtgg ccgatccgca
     7201 tgtctcgcgg cgccatgccg agatcgttgg cggcagcggg aacgtcgtca tccgtgattt
     7261 gcagagtcgc aacggcacgt atgtcgatgg catcgcggtg aaggaggcga tccttcagga
     7321 gcgagccacg atccgaatcg gcggcacgac gatccggttc gagaccgaag cccccacctc
     7381 ggccgccggg cccagacgga tgggcgaagc cgtcggctcg tcgcccgaga tgcagcgcgt
     7441 gttcgagatg ctcgaacggc tcgcgccatc ggatctgacg atcacgttgc tcggcgagac
     7501 aggtgtcggc aaagacgtgc tcgccggcgc ggtgcacgac gcgagcgcgc ggcgcgatgg
     7561 tccgttcatc gtgtttgact gcggcgccgc tgcaccgacg ctgatcgaga gcacgctgtt
     7621 cggtcacgag aagggttcgt tcacaggcgc gaatgcaacg gtcgtcggcg cgttcgaacg
     7681 cgcgcatggt ggcacgctat tcttcgacga gattggtgag ctcagcctcg acctgcagcc
     7741 caagctgttg cgcgcgctcg agcaacgccg gatccagcgc atcggctcga cggacgagat
     7801 tcccgtcgac gtgagaattt tggcagcgac gaatcgcgat ctcgaggccg aggtcgcggc
     7861 gggcaggttt cgccaggatt tgttcttccg gctgtcggcc gccgttgttg ccgtgccgcc
     7921 actacgcgcg cgcctcgccg atctgccaga gctggtcgac gcgatcctcg ctacggaggg
     7981 actgcggttg cgcgtcaccg ccgacacgct cgaggcactg gcgacctacg actggccggg
     8041 caacgtgcgc gagcttcgca acgtgctcac ggctgctgct gcgatggcgg acgccgactt
     8101 gctcgagccc aagcacctgc tgttcttcaa acagcgacgt cgcaacgcga cgctcgcgga
     8161 ctttccgctg ggcggccgtt cgctcgaatc gatcgagcgc gccgcgatcg tgcagacgct
     8221 caaggccgcc gacggcaaca aggtcaaggc ggcaaaggcg ctcgggatcg cggcgtcgac
     8281 gctgtacgag aagatgcggc gctaccgcat cgcctgagcg gcctgccccc agcgttgcat
     8341 ccctcggtcg acagtcacgc atcgagtcat ttgctagacg tgcgaacgct cgcgcgctag
     8401 ccggtggcgg cggctttgca atttgcgctg gcatgcgcat ccctgccagc actcttgcgc
     8461 tcgtcacgtg cctcgcggtc agtggatcgg cggacgcgaa gccgcaccgc cacggtcgcc
     8521 accacaaggt gaagaaggcg aagcatcgca aggtcgcgcc accggtcgag cgcgtacgcg
     8581 aaaccgacga cggcgagtcg gatcgcgatc gtgtggcgat cgcgatgcca gatgccgacg
     8641 acgagacgat cgaattcagc gacgacgacg tcgatgccga ccgcgacgac cgcgacgaac
     8701 gcgacgaccg cgacgaggcg cccgtgacgc tgcgcaagac cgcggtgtcg cggacgaaag
     8761 actggcacgt cgccatcgga ccgtacctgt gggcgtcgtc ggtcgatgcc aatgtctcgc
     8821 tcggctcggc gaacgtcgct gccggggtcg acttcatcga ggtgacccgc cacgcgcgct
     8881 acggcgccga agtgctcggc gaggcgcgtt atcgacggtt ctcgcttgct ggcgatgtga
     8941 tgtacggggt cgtcgcgctc accggcgagc gcgaggtcgg gccgctgatg gtgacgctcg
     9001 gcggtcaggc gagcagcctg ctcgtcgacg gcacggccgg ctatctggtc accggcgacg
     9061 atcacgcgct gttgtcgctc gaagcgcgcg gcggcgttcg ctaccagcgc accgaggttg
     9121 ctggctcggt cgggatcgat ggcaacccgg tgcagtcgac gtccgttgtc gatgccggag
     9181 gcgacgcggt cgctggtgca cgagcggtgt tgcggccgtg gaatcggttc tacgtctcgg
     9241 gtgcggccga cgtcggcgtg tacggcgtgt cgaatagcac ctggtcggcg tcggtcgacg
     9301 cgagcgtgcg catcggttcg cacgtgctgg tgtcgctggg ctggcgcacg ctcacgatcg
     9361 atcgcgcgac catcgccgtg acgatgcatg gtccgcgcgg tgccgtgcag ctcgtgttct
     9421 gatcggaatc gagccccatc gatgtctact tggggctgcg actcgactgc gcgtgatgcg
     9481 caagccggcc cgggccgcgt gggctaggcc cgcagaagcc catggtcgac ggaggtccgc
     9541 caaccgtggc ctcgtgtttg ctgaagtgtc gttcatgcgc atcacctgct ggggatctgt
     9601 cgtgttcggg attgtcgcgg ctgcctgcgt cggaccgctg ccgccaccgc cggagctcac
     9661 cgtgacgtca ccgcagcgag gtctggtgca gggcgaggcg gggcagatcg tcgtgaaggg
     9721 aaccgcgctg ccaggcccga gcggcgatcc ggtcgcacgt gtcacggtca acaacacccc
     9781 cgcgttgctt gcggccgatg gctcgttcag cgcggtcgtc aacgttcccg cgggtgcgat
     9841 gctgctcgag acggtcgcgg tctcggatgc gggcgcgaag gcaaccgacg cgcgggccgt
     9901 gcaggtcggt cagctccgcc cggtcggcac gagcatcgac cgcgctgtca ctgcggcggt
     9961 gtcggccgac gcgttcgcga agttgtcagc ggccgcggga ccgatcatca agagcacgaa
    10021 tctgatggcg ctgatcgcgc aggtgcagcc gaagctctgg gacgacatcg caaacgtcca
    10081 gctcacgatc acgaagctcg cgttcgctga cgtcaagatc tcgctcgcgc cggtcgatgg
    10141 tgggctgcag ttctcggcga agatcgacgg gctcagcgtc gccgcgaacg ctgcttatgg
    10201 tggcgtcctc gttcccgacg gcacgacgtc ggtgagcgcc accgccgaca ccgtgacgat
    10261 tgcagggacg ctcgtcgtga cgcccgccgg cacggccggt ttcaccacct ctatcgcgtc
    10321 gccgctcgtg ggcaccacga atctccgcct gcaagcgtcc gggctcgtcg gccagatcct
    10381 cgacctgctc aacaacaacc tcgcatcgac aatccagcgc atcacgacga gcactgccga
    10441 gaaggcaatg gcgccgttga tcacgaaggc gttcggcgca ctcgcgggcc cgcaacagct
    10501 cgacgtgctc ggtaagaagc tcgagatcca agcatcgccc agtgcggtcc agttcagcca
    10561 cacgggcgcg ctcgtgacgc tgaacctgca ggtcaagatc gcgggcagcg agtcgagccc
    10621 gggcttcatc tacacgccga atggtatgcc gacgatgaac gtcggcactg gcgtccagct
    10681 cggcctcgcc gacgatctga tcaacgagct cctcgccgag gtccacgcgc ttcagctcct
    10741 aaacattcac ctcgacgagg acttcggcct gttcgacgcg gcggatttca agctcacatt
    10801 gcccccgatg atcagcgcga acaacagcga cggcacgctg cggctcgtgc tcggcgatat
    10861 gatcgccacg ttcaccgatc acggcaatcc ggtcattcgt gccgcggtca atgcgcagat
    10921 cgacctcgcg attctgcgcg ggaccagtgc ccaagagatc gcgctgcagt tcggcaaggt
    10981 ccacgcgtac gtcaacgtcc tcgacaacgc cgagagctcc gacgacttgt ctggaccgac
    11041 gaaagacggc atcggcgtcc agctcgacag gctgagcaag ttcatgatca ccgtgccggt
    11101 gccgtcggtc gccggcgtgt cgctcgacaa cctcgcgctc cgtgctgaca gcggctacgt
    11161 cgtcgtatcg ggccagatcc actgacgcgt cgcgtggcac gagcctggca ctgagaacct
    11221 tgcgtgactg cgagggagaa ttgccgtggg cagtgaagcg attgcgtacc cgatgccagg
    11281 caccgccgcc ggccgcgaga cttctacgcg tgcggctgtg cgcggcgccg tctggatgct
    11341 cctctatctg ttctttattc tcgcgcctct gttcgcgctg ctcctgggcg ctcacccgcc
    11401 ggcgcgctcg ttctggacag aattctcgat tgcgatcggc tacgcaggcc tcgcgatcat
    11461 gggactgcaa ttcgctctta ctgcgcggtt ccggtacgtc accgcaccgt ggggcgagga
    11521 cgtcatctat cactttcacc ggcgggtctc gttgctcgcg gtcggcctcg tcatcgcgca
    11581 cccgatcatc ctgttcatcg agcgcccaga attgctcgcg ctgctcaatt cgtttcaggc
    11641 gccgtggcgg gcgcgcatgg ccgcactatc gacgtactcg ctgatcgcgc tcgtggcgat
    11701 ggcgttgtgg cgtacgaagc tgaggctgag ctacgagacc tggcacgtca cccacgtcgt
    11761 gctcgccgtt gtcgccgtgg ctgccggcgt cgcgcatatg gtgggttgga gtttctattt
    11821 cgaggacccg tggaagcgcg ggttgtggct cgggctgacc gtgttttgga tcggcctctt
    11881 gggttacgtc cgcatcatca agccgctgtt tgtcatccgc aggccctacc gcatctcggc
    11941 ggtgcgcaag gaacgcggca acacgtggac gttggtgatg aagccggaag gtcacgcc
//
LOCUS       DASUFX010000564         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_89764,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000564 DASUFX010000000
VERSION     DASUFX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89764"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFV99_22650"
     CDS             complement(<1..180)
                     /locus_tag="VFV99_22650"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5062195.1"
                     /translation="MSDRRERMVSDQLRRRDIADPAVLAAFSVVPREAFVDPESEPWA
                     YEDGPLPIGYGQTISQ"
     gene            complement(228..596)
                     /locus_tag="VFV99_22655"
     CDS             complement(228..596)
                     /locus_tag="VFV99_22655"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX5062196.1"
                     /translation="MLRVLLVFGAGGAGCVARYLIALWVGPRSFPYATLIVNLVGSFL
                     IALILEASLRVASFSPNLRLALTTGFMGGLTTYSSFNYETSALAANGSTGRALINVGA
                     TLIGCVLAGLAGLWLARRLT"
     gene            complement(639..1100)
                     /locus_tag="VFV99_22660"
     CDS             complement(639..1100)
                     /locus_tag="VFV99_22660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5062197.1"
                     /translation="MLPKNILVPTDLSEGAEEALDYACELARQFGATVHLLHVIGIPT
                     LGVPELGVALTSTVIDSMVRENQEALERLVDRKRTMATFGAPLLRTGDARDLINQTAK
                     ELTADLIVMGTHGRRGVSRALLGSVTETVVRTAPCPVLTVRPNVIHHEAAA"
     gene            1158..2579
                     /locus_tag="VFV99_22665"
     CDS             1158..2579
                     /locus_tag="VFV99_22665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062198.1"
                     /translation="MRLTRKFIAALVISVALVAMIQGFFDYRRERDVFDNQMRAEATA
                     LGRALARGMAEVWERDGEAAARQFLLTSSSRSEHIRARWVWLDATSRDAPSIPREQLA
                     MLDDGQVVSVKQQGVLLTYIPVKVPESRDGGLEIAQPLGELYKFTGESLRNGIIASAS
                     AVAIAGLLALVLGVVFIGRPISRLAEKARRVGTGDLSGPLVLSQRDELGQLANEINLM
                     CERLAAERSARELATEQLRHADRLTTVGKLASGIAHELGTPLNVVQGRARLIRDKEVE
                     GAEVGESARIVLEQAERMTALIRQLLDFARPRALHKTSLGVDALVTRVCELVGTIARK
                     ANVKLAPSAQNAGLRVDADEGQLHQVLTNLVINAIHAMPDGGTVEIVTSLRDATPPPY
                     IESTDTSWIAVAVRDTGTGMDAATRERIFEPFFTTKQVGDGTGLGLSVSWGIAREHGG
                     WIDVVSEPAAGSTFTVYLPRGQA"
     gene            2576..>2764
                     /locus_tag="VFV99_22670"
     CDS             2576..>2764
                     /locus_tag="VFV99_22670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5062199.1"
                     /translation="MKVFVVDDEKEMTDLVQLGLKKRGFQVVTFGNAADTLSALGEHD
                     VDVVVTDLNMKGMSGLELC"
BASE COUNT          504 a          903 c          902 g          455 t
ORIGIN      
        1 ttgcgagatg gtctgcccgt atccgatcgg aagcggacca tcctcgtatg cccatggctc
       61 gctctcgggg tcgacgaacg cctcgcgtgg caccacgctg aacgctgcca gcactgccgg
      121 atcggcgatg tcgcgcctcc gcaactgatc gctgaccatg cgttctcgcc tatcgctcat
      181 cctgcccacc tacgatgttg gccacatttg cagatagagg caatgcgtca ggtgagccga
      241 cgagcgagcc aaaggccggc gagtccggcg agtacgcagc cgatgagggt cgctccgaca
      301 ttgatcagcg cacgtcccgt cgagccattc gccgcgagcg ccgaggtctc gtagttgaag
      361 ctcgagtagg tcgtcagacc gcccatgaag ccggtcgtca gcgcgagccg caggttcggt
      421 gagaacgaag caacgcgcag cgacgcttcg aggatcagcg cgatcaagaa cgagccgacc
      481 aggttcacga tcagcgtcgc atacggaaac gatcgcggac cgacccacag cgcgatcaga
      541 tagcgcgcga cgcagccggc accgccggca ccgaacacga gcagcacgcg cagcatgctg
      601 tgacgctact acgaagccgc ggccgctgga atttcagcct acgcggcagc ttcgtgatgg
      661 atcacattcg gacggacggt cagcaccgga cacggagccg tgcgcacgac ggtctcggtc
      721 acgctgccga ggagtgcgcg cgaaacgcct cgccggccat gcgtgcccat cacgatgaga
      781 tcggcggtga gctccttcgc ggtctgattg atgagatccc gcgcatcgcc ggtgcgaagc
      841 agcggggctc cgaacgtcgc catggtccgc ttccgatcga cgagcctctc gagggcctcc
      901 tggttctccc gaaccatcga gtcgatcacg gtgctggtca gcgcgacccc gagctcgggc
      961 accccgaggg taggaatgcc aataacgtgg agcaggtgca ccgtcgcgcc gaactggcgt
     1021 gcaagctcgc acgcgtaatc gagcgcctct tcggctccct cgctcaaatc ggtcggaacg
     1081 aggatgtttt tcggcaacat gacaatccca agctgttcac gaagcgggcc aaggatgcgc
     1141 tacgttttgt cacgttcatg agactgaccc gtaagttcat cgccgcgctg gtcatcagcg
     1201 ttgccctcgt tgcgatgatt cagggtttct ttgattaccg gcgcgagcgc gatgtcttcg
     1261 acaatcagat gcgcgccgag gcgacagcgc tcggtcgcgc gctcgcgcgc ggcatggccg
     1321 aagtctggga gcgcgacggc gaagcggcag cccgtcagtt cctgctcacc tcgtcgagcc
     1381 ggagcgagca cattcgcgcg cgctgggtct ggctcgatgc gacaagccgc gatgcaccga
     1441 gcatcccgcg cgagcagtta gcgatgctcg acgatgggca agtcgtcagc gtgaagcaac
     1501 aaggcgtgtt gctcacgtac attccggtga aggtgcccga gtcgcgcgac ggcggcctcg
     1561 agatcgcgca gccgctcggc gagctctata agttcaccgg cgagtcgttg cgcaacggca
     1621 tcatcgcgtc ggcaagcgcc gtcgcgatcg ctggtctgct cgcgctcgta ctcggtgtcg
     1681 tgttcatcgg ccgcccgatc tcgcggctcg cggagaaagc gcgccgcgtc ggcaccggtg
     1741 atctgtccgg cccgctcgtg ctgagccagc gtgacgagct cggccagctc gcgaacgaga
     1801 tcaacctcat gtgcgaacgg ctcgccgccg agcgcagcgc ccgcgagctc gcgaccgaac
     1861 agctgcggca cgccgatcgg ctcaccaccg tcggcaagct cgcgtctggg atcgcgcacg
     1921 agctcggcac cccgctcaac gtcgtgcagg gacgtgcgcg cttgatccgc gacaaggagg
     1981 tcgaaggcgc ggaggtcggc gagtccgcac gcatcgtcct cgagcaagcc gagcgcatga
     2041 ctgcgctgat ccggcagctt ctcgatttcg cgcggccgcg tgcgctgcac aagacttcgc
     2101 tcggtgtcga cgcgctcgtg acgcgcgtgt gcgagctcgt cggaacgatc gcgcgcaagg
     2161 cgaacgtgaa gctcgcgccg tctgcgcaaa atgctggatt gcgcgtcgat gccgacgaag
     2221 gtcagcttca tcaagtgtta acgaacctcg tgatcaacgc gatccacgcg atgcccgacg
     2281 gcggtacggt ggagatcgtg accagtctgc gcgacgccac gccgccgccg tacatcgaaa
     2341 gcaccgacac gagctggatc gccgtcgcgg tccgcgatac cggaaccggc atggacgccg
     2401 cgacacgcga acggatcttc gagccgttct tcacgacgaa gcaggtcggt gacggcaccg
     2461 gtctgggcct cagcgtgtcg tggggcatcg cgcgcgagca cggcggctgg atcgatgtgg
     2521 tgtcggaacc cgctgctggc tcgacgttca cggtgtactt gccgcgaggt caggcatgaa
     2581 ggtcttcgtc gtcgacgacg agaaggaaat gaccgacctc gtccagctcg ggctcaagaa
     2641 gcgcggcttt caggtcgtca cgttcggcaa cgccgccgac acgctctccg cactcggcga
     2701 gcacgacgtg gacgtcgtcg tcaccgacct caacatgaag gggatgagcg gcctcgagct
     2761 gtgc
//
LOCUS       DASUFX010000565         7689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1202102, whole genome shotgun sequence.
ACCESSION   DASUFX010000565 DASUFX010000000
VERSION     DASUFX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202102"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VFV99_22675"
     CDS             <1..1523
                     /locus_tag="VFV99_22675"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062200.1"
                     /translation="KLFPGDEAEDIMKNIESMPIPKAASIRPGLPSRLDEILAGPLAR
                     KPIDRPGRPAIVLRQLIELSYESSIMATAMDVSEAVASVLPAKRLEGPGQQLDDVIRR
                     QLGAAERERSVARRTAITDGKPPPTHTHDKLDHSTGMIRRIGLDGLSQLEEIEVDPTA
                     VAAPRAKRASESGAVKALGGPSSPDSALHELLDANFDPDRRTEPGGPPTGQVPKSDGT
                     AAVPKTATKTSSTRSSRTGLWVVIALVVLAGGGFGVWKLMQKPTTNVVTPTAKDAGTG
                     IHLGIVEIDPDPDGATGYIADEAGTIAVDGKFGPTSHKKPLRLRVEAGKLFKVHIDRE
                     GYESYDTQRTINAAETVVIAPRLNKAKAALKVTTTPDGAEVAFKGKLLGETPLSRTDL
                     EDAKGELVITKAGFDTYKQKIELVAGKTVEITQTLKAAAKLGSLHIRLKGGGWGDIYL
                     NGAMLGRAPTREPLRVPIGKQTFQLVNSGNASKKIKWTVTCEVSATETNTCTTQMP"
     gene            complement(1527..1832)
                     /locus_tag="VFV99_22680"
     CDS             complement(1527..1832)
                     /locus_tag="VFV99_22680"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5062201.1"
                     /translation="MPTVTIITGEGDPIVVDAPEGSTLFEAGAKVAAGIDTACVGKGT
                     CGLCRVKIIAGAEHLNPFTDEERKHLGNVYHLTKVRLSCRSKVSGDVTIEIVRKKKK"
     gene            complement(1820..3262)
                     /locus_tag="VFV99_22685"
     CDS             complement(1820..3262)
                     /locus_tag="VFV99_22685"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5062202.1"
                     /translation="MASSPLPDSIATAYDPERFRQSGHELIDAMADALARWQRREGVV
                     LPWRTPDDALAMWSRKGFGGTDLVVQLRRIMASSTALYHPRCMAHQVPPPLPGAALAE
                     LVSALLNNGMAVYEMGPAAVPIELSVVDWMCQKLGYAEGAGGVLTSGGSLGNLTALLA
                     MRQAAAGFDVWSQGAHAGPPLAVVTSTDAHYSVARTLRVMGWGDGGVIAVPVDDKHRL
                     TASAVFRTLESVRDRKVIGIIAAAGSTATGAFDPLEELAEVAQRHRLWLHVDAAHGGG
                     VALSQAHRHKLAGIERADSVVWDAHKMMMMPALVTAVLFKRGSHVYDAFAQQASYLFA
                     QQAREETWWDLGQRTLECTKRMMAIEVWAALRVHGEGFFNDVVDHLIALAAQLAAKVR
                     AAGDFELAIEPELNIVCYRHRPEGVAPGPVLDEHNRKLRQRLVEDGRFYIVGTQLPGG
                     YFLRSTIMNPLIEPADFDDLLAHLRTLCRP"
     gene            3297..3971
                     /locus_tag="VFV99_22690"
     CDS             3297..3971
                     /locus_tag="VFV99_22690"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5062203.1"
                     /translation="MALQIGMLLFPKLTQLDLTGPQEVLARIPDAKVHIVAKTREPVT
                     AETGLTLVPSTTFAELPQVDLLFVPGGAGQIDAAEDDATIGWLRQVGAQARWITSACT
                     GSLLLGCAGLLRGYRATTHWAFFDLLPLVGAIPTDKRVVVDRNRMTSGGVTAGIDIAL
                     TIAAEVAGREHAEAIQLSLEYDPEPPFRAGHPNVARPDLVERVRATIAKRHAQRKAQL
                     EQLARG"
     gene            complement(3968..5854)
                     /locus_tag="VFV99_22695"
     CDS             complement(3968..5854)
                     /locus_tag="VFV99_22695"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEX5062204.1"
                     /translation="MRATNSVWVASLLLFFVACGPDGRDNPGNCPGICSSLGFQECND
                     GVYSDPVACGPDQVCDPNAGCVVCPPDELYCFGDTSNEVWRCNHDGTGGTHVETCDAD
                     SVCSGGECKTPCEAAADNPSNLGCDFWAADLDNESSTLGPIVNDAAAQQFAVVAANNN
                     DYPVVVTVTKNAARVGEPLNEQAVASVTVPARGVQRIDLPQHEVDGAMGQNGTYTKNS
                     GSGTFVSPHGYHIVSTGPVVVYQFNPIIQQYSNDASTLIPTTAIGFDYVIIGYNTANP
                     CGNPQLPIESIPDHTAITILAAEDDTTVTVTPTHPIKASGGDSGFAIAMTAKGTPLTF
                     KLSRYTVVNLESEQPNDGNVFACAMSGQNGDFTGSQIHADKPIVVFTSGERGIGFGGA
                     ENIVYPPDWDEQNDDTCCTDHLEEQLLPVTALGKEFAIARSPIRSTDPTWKEPDIVRV
                     VATVDGTIVTTNLPAPNDSFTLDANQQRSFGATVGFGLKASNAVEVSTYLVSQHFVKS
                     GYTGDPSQLLIPAAEQHRKDYVFLIPGTFDSNYIVLAKPVDAAIMVDGFPLAMQEFAN
                     CVTGPIGMVAGVAYDQVTCKAMEGRHTVSADKPFGLSVYGYYNVGSYAFVGGSDVKII
                     NPIL"
     gene            complement(5958..6098)
                     /locus_tag="VFV99_22700"
     CDS             complement(5958..6098)
                     /locus_tag="VFV99_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062205.1"
                     /translation="MASLSVAAGEMRAAEGYLLELLAWCAFADRSVIDVSGDGGEGPA
                     HA"
     gene            complement(6290..>7689)
                     /locus_tag="VFV99_22705"
     CDS             complement(6290..>7689)
                     /locus_tag="VFV99_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062206.1"
                     /translation="SRAARLEVMSDALHSELSRVSELAYRACSASPTCRDYTRAEVET
                     ALAEIFAGYPTYRCYLSEQRRDDIDRARLASAVTAAHDAKPGLDRDLLAYLEGVLAFE
                     IPGDAATELAHATQQVTGAITAKGDEDTLLYRQVRLLARCDVGAELSQWAHSASAFHH
                     VLVNGRSRNLLATSTHDTKRSEDVRARIAVLSELPTEWSAAVMRWRDRAERYWIGTRE
                     PVIEYTLWQTLVGAWPLPCERAKTYARKAARESRLRTSWRHPDEAFDNALDHWLEGVY
                     SDPELLAEVAAFAERLRPHGDRNSLAQLLIKLTAPGIPDFYQGTELYDDSLVDPDNRR
                     PVDLALRRDKLRALERASVQDVAGDLSAMKMWTIRRVLGLRRRHPARFAGGYRALDAA
                     GPHAHRVFAFARGDDVVTIVPRLGVHADGYRDTTLEIPSGTWREVLSDQTFSGGTCAI
                     AQLWRALPIALLTNS"
BASE COUNT         1333 a         2576 c         2541 g         1239 t
ORIGIN      
        1 agaagctgtt ccccggcgac gaggccgagg acatcatgaa gaacatcgag tcgatgccga
       61 ttccaaaggc ggcgtcgatt cgcccgggcc tgccgtcgcg gctcgacgag atcctcgccg
      121 gtccactcgc gcgcaagccg atcgatcggc cggggcgtcc cgcgatcgtg ctgcgccagc
      181 tgatcgagct ctcgtacgag tcgagcatca tggcgaccgc gatggacgtc tcggaggcgg
      241 tcgcgtcggt gctgcccgca aagcggctcg agggtcccgg tcagcagctc gacgacgtga
      301 tccgcagaca gctcggcgcc gcagagcgag agcgctcggt cgcacgccgc accgcaatca
      361 ccgatggcaa gccgccgccg acgcacacgc acgacaagct cgatcactcg accggcatga
      421 tccggcgcat cggcctcgac ggtctctcgc agctcgagga gatcgaggtc gatccgacgg
      481 cggtcgccgc accgcgcgcg aagcgagcct cggagtcggg cgccgtgaaa gcgctcggcg
      541 ggccgtcgtc gccggattcg gcgttgcacg agctgctcga cgcgaacttc gatcccgacc
      601 gccgcaccga gcccggcggg ccgccgaccg gtcaggtgcc gaagagcgat ggcaccgcgg
      661 cggtgccgaa gacagcgacg aagacgtcga gcacgcggtc gtcgcgcacc ggcctctggg
      721 tcgtgatcgc gctcgtcgtg ctcgcgggcg gtgggttcgg cgtgtggaag ctgatgcaga
      781 agccgacgac gaacgtcgtg acgccgacag ccaaggacgc tggcaccggg atccatcttg
      841 gcatcgtcga gatcgatccg gatccggacg gcgcgaccgg ctacatcgcc gacgaggccg
      901 gcacgatcgc cgtcgacggc aagtttggcc ccaccagtca caagaaaccg ctccgcttgc
      961 gcgtcgaggc cggcaagttg ttcaaagtgc acatcgaccg ggagggctac gagtcctacg
     1021 acacacaacg cacgatcaac gcggccgaga cggtcgtgat cgcaccgagg ctgaacaagg
     1081 cgaaagcagc gctgaaggtc accacgaccc cggacggcgc cgaggtcgcg ttcaagggca
     1141 agctgctcgg cgagacgccg ctctcgcgca cggacctcga ggatgcgaag ggcgagctcg
     1201 tgatcacgaa ggccgggttc gacacttaca agcaaaagat cgagctcgtc gctggcaaga
     1261 ccgtcgagat cacgcagaca ctgaaggcgg cggcaaagct cggctcgctg cacatccgtc
     1321 tcaagggcgg tggttggggt gacatatatt tgaacggtgc aatgctcggc cgcgctccga
     1381 cgcgcgagcc gctccgcgtg cccattggca agcagacgtt ccagctcgtc aacagcggca
     1441 atgcgagcaa gaaaatcaag tggacggtga cgtgcgaggt cagcgcaacc gagacgaaca
     1501 cctgcacgac gcagatgccg tgaccgtcac ttcttcttct tgcgcacgat ctcgatcgtc
     1561 acgtcgccac tgaccttcga ccggcacgac aggcgcacct tcgtgaggtg atagacgttg
     1621 ccgaggtgct tgcgttcctc gtcggtgaac ggattcaggt gctcggcacc cgcgatgatc
     1681 ttcacgcgac acaggccgca cgtgcccttg ccaacacacg cggtatcgat accggccgcg
     1741 accttcgcgc cagcttcgaa cagcgtcgag ccttccggtg catcgacgac gatcgggtcg
     1801 ccttcgccgg tgatgatcgt tacggtcggc atagcgtgcg cagatgcgcg agcagatcgt
     1861 cgaagtcggc cggctcgatc agcgggttca tgatcgtcga gcgcaagaag tagccacccg
     1921 gcaactgcgt accgacgatg tagaaccggc catcctcgac gaggcgctgg cgtagcttgc
     1981 ggttgtgctc gtcgagcaca ggcccgggcg cgacgccctc gggacgatgg cggtagcaca
     2041 cgatgttgag ctcgggctcg atcgcgagct cgaagtcgcc ggccgcgcgc accttcgccg
     2101 cgagctgcgc cgcgagcgcg atcagatgat cgacgacgtc gttgaagaag ccctcgccgt
     2161 ggacgcgcaa cgcggcccac acctcgattg ccatcatgcg cttcgtgcac tcgagcgtgc
     2221 gctggccgag atcccaccac gtctcctcgc gcgcctgctg cgcgaacaga tacgacgcct
     2281 gctgcgcgaa cgcgtcgtag acgtgactgc cgcgcttgaa cagcaccgca gtcacgagcg
     2341 ccggcatcat catcatcttg tgcgcgtccc acacgacgga gtcggcgcgc tcgatgccgg
     2401 ccagcttgtg ccggtgtgct tgcgacagcg cgaccccgcc gccgtgcgcc gcatcgacat
     2461 gcagccagag ccggtgacgc tgcgcgacct cggcaagctc ttcgagtgga tcgaatgcgc
     2521 cggtcgccgt cgagccggcg gcggcgatga tcccgatcac cttgcgatcg cgcacactct
     2581 ccagcgtgcg aaagaccgcc gacgcggtga gccggtgttt gtcgtcgacc ggcaccgcga
     2641 tcacgccgcc atcgccccac cccatgacgc gcagcgtgcg cgcgaccgag tagtgcgcgt
     2701 cggtcgacgt gacgaccgcg agcggcggac ccgcgtgcgc gccttgcgac cacacgtcga
     2761 agccggccgc ggcctggcgc atcgcgagca gcgcggtcag gttgccgagc gagccgcccg
     2821 acgtgagcac gccgcccgcg ccttccgcgt agccgagctt ctggcacatc cagtcgacga
     2881 cggaaagctc gatcggcacc gccgccggtc ccatctcgta gaccgccatg ccgttattga
     2941 gcagcgcgct gaccagctcg gcgagcgcgg cgcccggcaa cggtggcggc acctgatgcg
     3001 ccatgcagcg cgggtgatag agcgccgtcg agctcgccat gatccggcgc agctggacga
     3061 cgaggtcggt accgccgaag cctttgcgcg accacatcgc gagcgcgtcg tcgggcgtgc
     3121 gccacggcag cacgacacct tcgcggcgct gccagcgcgc gagcgcatcc gccatcgcat
     3181 cgatcagctc gtggccgctc tggcggaatc gttccggatc gtaggccgtc gcgatcgagt
     3241 ccggcagggg ggatgacgcc atcgccgaca ggctaccgca cgcgtatgct caggtcatgg
     3301 cgctgcagat cggcatgctg ctgtttccga agctcaccca gctcgatttg acaggcccgc
     3361 aagaagtgct ggcccgcatt cccgacgcca aggtccacat cgtggccaag acccgcgagc
     3421 cggtgaccgc cgagaccggc ctgactctcg tgccatcgac gacattcgcc gagctgccgc
     3481 aggtggatct gctattcgtg ccgggcggcg cgggtcagat cgacgccgcc gaggacgacg
     3541 cgacgatcgg ttggctccgc caggtcggcg cgcaggcgcg ctggatcacc tcggcatgca
     3601 ccggctcgct gctgctcggc tgcgccggcc tcttgcgcgg ctaccgcgcg accacgcact
     3661 gggcgttctt cgatctgttg ccgctcgtcg gcgcgattcc gacagacaaa cgcgtcgtcg
     3721 tcgatcgcaa ccggatgacg agcgggggag tcaccgccgg catcgacatc gcgctgacga
     3781 tcgccgccga agtagcgggc cgcgagcacg ccgaggcgat ccagctgtcg ctcgagtacg
     3841 accccgaacc gccgttccgc gccggccacc cgaacgtcgc gcgtcccgat ctcgtcgagc
     3901 gcgtgcgcgc cacgatcgcg aagcgccacg cgcagcgtaa agcgcagctc gagcaactcg
     3961 cccgcggtta gaggatcggg ttgatgatct tgacgtcgct gccgccgacg aacgcgtagc
     4021 taccgacgtt gtagtagccg tagaccgaga ggccgaacgg cttgtcggca gagacggtgt
     4081 ggcgaccttc catcgctttg cacgtgacct ggtcatacgc gacgcctgcg accataccga
     4141 tcggaccggt cacgcagttt gcaaactcct gcatcgccag cgggaagccg tcgaccatga
     4201 tcgctgcgtc gacgggcttc gcgagcacga tgtagttcga gtcgaacgtg cccggaatca
     4261 ggaagacgta atccttgcgg tgctgctcgg cggctgggat cagcagctga ctcggatcac
     4321 cggtatagcc gctcttcacg aagtgctggc tgacgaggta cgtcgatacc tcgaccgcgt
     4381 tggatgcctt gagcccgaag ccgaccgtcg cgccaaacga cctctgctgg ttcgcatcga
     4441 gcgtgaacga atcgttcggc gccggcagat tcgtcgtgac tatcgtcccg tcaacagtgg
     4501 ccacgacgcg cacgatgtcg ggctccttcc aggtcggatc ggtcgaccgg atcgggctgc
     4561 gtgcgatcgc gaattctttg ccgagcgcgg tgaccggcag cagctgctct tcgaggtgat
     4621 cggtgcagca cgtgtcgtca ttctgctcgt cccagtccgg cggatagacg atgttctcgg
     4681 cgccgccgaa accgatgcca cgctcgccgc tcgtgaacac gacgatcggc ttgtcggcgt
     4741 ggatctggct gccggtgaag tcaccgttct ggcccgacat cgcacaggca aacacgttgc
     4801 cgtcgttcgg ctgctcggac tcgaggttga cgaccgtgta gcggctgagc ttgaacgtca
     4861 gaggcgtgcc cttcgccgtc atcgcgatcg cgaagcccga gtcgccgccc gatgctttga
     4921 tcggatgcgt tggcgtcacc gtcacggtcg tgtcgtcctc tgccgcgagg atcgtgatcg
     4981 ccgtgtgatc ggggatgctc tcgatgggga gttgcgggtt accgcatggg ttcgccgtgt
     5041 tgtagccgat gatcacgtag tcaaagccga tcgcagtcgt cgggatcagc gtcgatgcgt
     5101 cgttcgaata ctgctggatg atcggattga actgatacac gaccaccggc cccgtcgaca
     5161 cgatgtggta tccgtgcggc gacacgaacg ttcccgagcc gctgttcttc gtgtacgtgc
     5221 cgttctggcc catcgcaccg tcgacttcgt gttgcggcag atcgatccgc tgcacgccgc
     5281 gcgcgggcac ggtaacgctc gcgaccgcct gctcgttgag cggttcgccg acgcgggccg
     5341 cgttcttcgt caccgtgacg acgaccgggt agtcattgtt gttcgccgcg acgactgcga
     5401 actgctgtgc agcggcgtcg ttcacgatcg gcccgagagt ggacgactcg ttatcgaggt
     5461 ccgccgccca gaaatcgcaa ccgaggttcg acgggttgtc ggccgctgct tcgcacggtg
     5521 tcttgcactc gccaccggag cagacgctgt cggcgtcaca cgtctcgacg tgcgtgccgc
     5581 cggtcccgtc gtggttgcag cgccagactt cgttgctcgt gtcgccgaag caatagagct
     5641 cgtcgggcgg gcacacgacg cagccggcgt tcggatcgca gacctgatcc ggaccgcacg
     5701 cgaccgggtc gctatagacg ccgtcgttgc actcctggaa cccgagcgac gagcagatgc
     5761 ccgggcagtt gcccgggttg tcgcggccgt ctggaccgca cgcgacgaaa aaaagtaaca
     5821 gcgaagcaac ccaaaccgag ttggtggccc tcatcgcgcg cgaacgtcat ccagttcccg
     5881 aggccacgtc aacaacgatc tgcaaacccc cgcgctagtt gtgcacgtcc gtgcacaact
     5941 ccatccgttt cagcggatca agcgtgcgcc ggaccctcgc caccatcacc cgagacatcg
     6001 atgacactgc ggtccgcgaa agcgcaccac gcgagcagct cgaggagata cccctcggcg
     6061 gcgcgcatct cgccagcggc gaccgacagt gacgccatcc aagacagggt gcgtgcacga
     6121 gggcggagat cttgatcgag ctccgtcgtc ggattggttg atgaccagct cataatgacc
     6181 atcggttctt cacagaccgg gccaagacgg gccgaaaacc gctgctcgcg aagtccgcga
     6241 ttcgacactc tcggcggcgt gcgccgacgc gcaccgagca ggatcgctac taagagttcg
     6301 tcaaaagcgc gatcggtaag gcgcgccaga gctgggcgat cgcgcacgtg ccgccggaga
     6361 aggtctgatc gctaagcacc tcccgccatg tgccggacgg gatttcgagc gtggtgtcac
     6421 ggtagccgtc agcgtgcaca cccagacgcg gcacgatcgt cacgacgtca tcgccacgcg
     6481 caaacgcgaa cacgcggtgc gcgtgcggac ctgccgcgtc gagcgcgcga tagccaccgg
     6541 cgaagcgagc cggatgccta cggcgaagac cgagcacgcg gcggatcgtc cacatcttca
     6601 tggccgagag atcgcccgct acgtcctgca cgctggcgcg ttcgagcgcg cgtagcttgt
     6661 cgcggcggag tgcgagatcg actggacgcc gattgtcggg atcgacaagc gagtcgtcgt
     6721 agagctcggt cccttgatag aagtccggta tgcccggcgc ggtgagcttg atgagcagct
     6781 gggcgagtga gttgcgatcg ccgtgcgggc ggaggcgctc cgcaaatgcg gcgacctcgg
     6841 cgaggagctc gggatccgag tacacgccct cgagccagtg atcgagcgcg ttgtcgaacg
     6901 cttcgtcggg atgccgccac gaggtccgca gccgggactc gcgcgccgcc ttgcgcgcgt
     6961 aggtcttcgc gcgctcgcac ggtagcggcc acgcaccgac gagcgtttgc cacagcgtgt
     7021 actcgatcac cggctcgcgc gtgccgatcc agtatcgctc tgcgcggtcg cgccagcgca
     7081 tcactgcggc actccactcc gtcggcagct cggacaacac ggcgatccgc gcgcgcacgt
     7141 cctcgctgcg cttggtgtcg tgcgtcgacg tcgcgagcag gttgcgcgac cgtccgttca
     7201 cgagcacatg gtgaaacgcc gacgcagaat gtgcccactg cgacaactcg gcgccgacgt
     7261 cacaacgtgc gaggagccgc acctggcgat acagcagcgt gtcctcgtcg cctttggccg
     7321 tgatcgcgcc ggtgacttgc tgcgtcgcgt gtgcgagctc ggtggccgcg tctccgggaa
     7381 tctcgaacgc gagcacgccc tcgaggtacg cgagaagatc gcgatcgagc cccggcttgg
     7441 cgtcgtgcgc cgcggtcacg gcggaggcca ggcgcgcacg atcgatgtca tcgcgtcgct
     7501 gctccgacag atagcagcga tacgtcggat agcctgcgaa gatctcggcg agtgcagtct
     7561 cgacttcggc gcgcgtgtag tcgcgacacg tcgggctcgc gctgcacgcg cggtacgcga
     7621 gctcggacac gcgtgatagc tcgctatgca gcgcgtcgct catcacctcg agacgggcgg
     7681 cgcggctgc
//
LOCUS       DASUFX010000566         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_369212,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000566 DASUFX010000000
VERSION     DASUFX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369212"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFV99_22710"
     CDS             complement(<1..1259)
                     /locus_tag="VFV99_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062207.1"
                     /translation="MPRAYRTVRDHLDDIVGYARCVFEQAKATDPARLAELQFEIADR
                     FEQMEGSLELARIASMPLPLDRLRKAFKLEKEEVRCLYLLLGLAVSNEVRTLANNDGT
                     GSIEVLDRLVYGSSPARDRFADHLANDGRLFGYGLTEFADDNRSRFARPVRVSDRVVD
                     LAFGRDRAASELAGFGTLTIARPGQLLIPDDARREVQSALRRHTELGIGPVPVIRGVS
                     GSGRRALVASVANELGARMLIVHCGALPENLGAALTTIMREAILAGTVIVLADAEHLA
                     ADATQGRRDRAAAIDAAFAHYSGPLAITMAKDASPANLMQRRGAIVVDLPALTEAERN
                     ALWSRHVPADLAADVAARYRVSGGHIERASQAVKQRSDDPTLADVHAAVRSVVDDKLT
                     TLGVRIEWKQSWADLVLPDDSYEELRE"
     gene            complement(1300..>2162)
                     /locus_tag="VFV99_22715"
     CDS             complement(1300..>2162)
                     /locus_tag="VFV99_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062208.1"
                     /translation="PGMSAVDAGRASKAKVEDGSGYIYIGKHPIRYQFAFHSDDAGNV
                     RAFGFNVDEEGFATMKTAWGEPLAYKALVNDELAWFNPTAKIKATAESDPWNRTDPKT
                     KEYVDVPGYNVRFQRYMPLADVLGPDGIVQKPLIGNGVAEINARLPGALEIKSAAQNK
                     ADIDKLGLDERTKKQMEALGAGGASADIVLPETETTAHHLLVQPDWKDQKIVKYSFSL
                     PFGKDNAKVKNEILAQVAQSLGKPLSVTQDEFNKDRWVYEFKGAPGTKVTLRSDILGE
                     GWTLEVEPAK"
BASE COUNT          338 a          715 c          697 g          412 t
ORIGIN      
        1 atctcgcgca gttcctcgta ggagtcgtcg ggcagcacga ggtcggccca gctctgtttc
       61 cattcgatgc gcacaccgag tgtcgtgagc ttgtcgtcga cgacgctgcg caccgccgcg
      121 tggacatcgg caagtgtcgg gtcgtcgctg cgctgcttta cggcctgcga cgcgcgctcg
      181 atgtgcccgc cgcttactcg gtagcgcgcg gcgacgtcgg ccgcgaggtc ggctggcacg
      241 tgacgcgacc acagtgcgtt gcgctcggct tcggtgagcg ccggcagatc gacaacgatg
      301 gcgcctcgcc gctgcatcaa atttgccggc gacgcatcct tcgccatcgt gatcgcgagc
      361 gggccgctgt agtgcgcgaa cgcggcatcg atcgcggcgg cgcggtcacg gcgaccttgc
      421 gtcgcgtccg cggcgagatg ctcggcatcg gcgagcacga tcacagtgcc ggcgagaatc
      481 gcttcgcgca tgatcgtcgt cagtgccgcg ccgagattct cggggagcgc gccacagtgt
      541 acgatcagca tgcgcgcgcc gagctcgttc gcgacggacg cgaccagtgc gcgacgaccc
      601 gatccgctga caccacgaat caccggtaca ggaccaatgc cgagctcggt atgtcggcgc
      661 agtgccgatt gtacttcgcg gcgagcatcg tcagggatca ggagctgtcc ggggcgcgcg
      721 atcgtgagcg taccgaagcc agcaagctcg ctcgccgcac ggtcgcgacc gaacgcgaga
      781 tcgacgactc gatccgacac gcgaaccggc cgcgcgaacc ggctgcggtt gtcgtcggcg
      841 aattcggtca ggccgtatcc gaacagtctg ccgtcgttgg cgagatggtc cgcgaaacgg
      901 tcgcgtgcgg gcgacgaacc gtacacgagc cgatcgagca cctcgatcga gccagtgcca
      961 tcgttgtttg cgagcgttcg gacttcgttc gacacggcga gcccgagcag caggtacaga
     1021 cagcgcacct cctccttctc gagcttgaac gctttgcgaa ggcgatcgag cggcagcggc
     1081 atgctcgcga tccgcgcgag ctcgaggctg ccctccattt gctcgaagcg gtctgcgatc
     1141 tcgaactgta gctctgcgag ccgcgccggg tcggtcgcct tcgcttgctc gaatacgcag
     1201 cgtgcgtatc cgacgatgtc atctagatga tcgcgaaccg tccgatacgc ccttggcacg
     1261 ccacagacat tacccggatc gcgccgacga gcgaagcggc tacttcgccg gctcgacttc
     1321 gagcgtccag ccctcgccga ggatgtcgct gcgcagcgtg accttcgtgc ccggcgcacc
     1381 cttgaactcg tagacccagc gatccttgtt gaactcgtcc tgcgtcacgc tgagtggctt
     1441 gccgagcgac tgcgcgactt gcgcgaggat ctcgttcttc accttcgcgt tgtccttacc
     1501 gaacggaagc gagaacgagt acttcacgat cttctgatcc ttccagtccg gttgcacgag
     1561 caggtggtgc gcggtggtct cggtctcggg cagcacgatg tccgcggacg cgccgccggc
     1621 accgagcgct tccatttgct tcttcgtgcg ctcgtcgagg ccgagcttgt cgatgtctgc
     1681 cttgttctgc gcagcgctct tgatctcgag cgcacccggc agccgcgcgt tgatctcggc
     1741 gacgccattg ccgatcagcg gcttctgcac gatgccgtcg ggcccgagca cgtcggcgag
     1801 cggcatgtag cgctggaacc gcacgttgta gccaggcacg tcgacgtatt ccttcgtctt
     1861 cggatcggtg cggttccacg gatccgactc ggcggtcgcc ttgatcttcg cagtcgggtt
     1921 gaaccacgcg agctcgtcgt tgacgagcgc cttgtacgcg agcggctcgc cccacgccgt
     1981 cttcattgtc gcgaagcctt cctcgtcgac gttgaagccg aacgcgcgga cgttgccggc
     2041 gtcatcggag tggaacgcga actgatagcg aatcggatgc ttgccgatat agatgtagcc
     2101 gctgccgtcc tcgaccttgg ccttcgacgc tctgcctgcg tcgactgccg acatgcctgg
     2161 cg
//
LOCUS       DASUFX010000567         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_90476,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000567 DASUFX010000000
VERSION     DASUFX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90476"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..2052
                     /locus_tag="VFV99_22720"
     CDS             61..2052
                     /locus_tag="VFV99_22720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062209.1"
                     /translation="MGTSESRPFASSSDGVRALCTRCGKTWPAETMSCSSCGAELGGE
                     TVLDDVHRVDTLPLDARVPNATSSQSDTTPEAEAFGEETPIKSKAFHASELPLFAERS
                     DAEIKQVTALVPRKPIVPATALPTGSSSSDPPTRLLRGFEQHIELPNGSVVDDYEIDV
                     KLGAGAMGVVYGAKHVKLGRRVAIKVIAPTMGEDPQALGRFEREARALASLHHPNIVD
                     VSSFGTLSDGRSYFVMEYLVGETLEDRLVRGRIPLDEALDTLDQMARALEGAHTQGIV
                     HRDLKPSNTFLVRIPREQRSIVKLLDFGLAKLAVADGVEKTASGAVIGTALYISPEQA
                     RSPNVDGRTDVYALGCIAYELVLNRHPFPEARTPTAAIAAHLTEPVPQPRTIWPGIPA
                     ALDLLLFSMLAKDPSYRPTLAQVRNVIASVRSPTTAAGREMRMSTEPTRRVDLRSRVW
                     MAALIAFTLLVGIMIGAVALGRKSSNGNAPREAPAGLGAASRNSENRATPPTPLEPAP
                     VAPSPVAPSPVAPSPTAPSSVIETSVAPIPAPVADTPKVTKPAGRSIPHSPRQTAASS
                     AASDGALALDSKPKMDVLVDGEPKGQTPVFGLKLPVGRHKITLINDTYSIKESFRVDI
                     KPARVERIVKDYSDRIPEVKKPDARTTINPFEKQGSAAR"
     gene            2241..2720
                     /locus_tag="VFV99_22725"
     CDS             2241..2720
                     /locus_tag="VFV99_22725"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEX5062210.1"
                     /translation="MLVILHAGQTGVERGAHRAATATAIFVAGFMPLDKRDELGPIPR
                     EIAEVLTPCPERGVRSAVRSNIKIASAVLLVVPDQSRVERFTAMTAVLQAIRAARIPS
                     LICDPATNLADVTTWGGRVCSTSGSRRILVTGPRETRWMDGESVARRMVTAIGMTLK"
     gene            2783..>4518
                     /locus_tag="VFV99_22730"
     CDS             2783..>4518
                     /locus_tag="VFV99_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062211.1"
                     /translation="MPVIQRSLARYLLVGSVLAVVVNVVINAAVGNALYAHVATIPLT
                     GDPSIAGDTIVGAFLIAFFTVVVVAPATRREIRSGRVRGGRKLTLPGWFARRPLLAAV
                     AGGLVSATLIGGGAVAIIAAIGAAPMSGHAFLAFKIAFAGVWGGLAAVLVAALAAAGE
                     PEPPVDDRWCRDPAAPVAVSYPCDFFDKGGLAVTSARHGCSGTPTWQLVVTGVPDPAA
                     VRAALGDLLVRYPALATRVQSLDAIPEYARRFRYAAAPTDVDSIFELIDLRGRPSSEL
                     EAVIHRVWNRHLDQFRDAPISLTMAITDDEHCRLLFRQHHGIADGRAFIGLLNDFVAF
                     VRARQSGAAPAPAALEPIGRRGELDALGLSPTTRARYALAGFASLTASIVKAIVRPSA
                     ILVQNESADYTGPNGAVRWIVDDTVLDAWQRARKQLGVSQSALFMAALFVATQRWHRE
                     LGRRLGRVNASLVMETRPRDGFFVSFANHLATLEVELHLERDLDPADAARAIHAQLKR
                     QLDRNRPLKRLVCERALVAGMPLDKLQALVFDAKRPAFNLNLSNLIPLDFPVLSGPAW
                     TVDEVLITTPVTP"
BASE COUNT          793 a         1534 c         1403 g          788 t
ORIGIN      
        1 ggaacttcga cgcgaccgac accgccgttt cccggccgct attcagatac gcttgatcac
       61 atgggcacgt ctgagagccg gccgttcgcg tcgagcagtg acggtgtccg cgcactgtgc
      121 acgcggtgcg ggaaaacgtg gcccgcggag acgatgtcct gttcatcgtg cggcgccgag
      181 ctcggcggcg aaactgttct cgacgacgtc catcgggtcg acacgttgcc gctcgacgca
      241 cgggtgccga acgcaacatc ctcgcagagc gacaccacgc cagaagccga agcgtttgga
      301 gaagagaccc cgatcaagag caaggcattt cacgcgagcg agcttccgtt gttcgccgag
      361 cgctccgacg ccgagatcaa gcaagtcacc gcgctggtgc cgcgcaaacc gatcgttcct
      421 gcaaccgcgc ttcccacagg tagtagctcg agcgatccac cgacgcgact gctaaggggc
      481 ttcgaacagc atatcgagct accaaacggt tccgtcgtcg atgactacga gatcgacgtg
      541 aagctcggcg caggtgcgat gggagtcgtg tatggcgcca agcacgtaaa gctgggtcgt
      601 cgcgtcgcga tcaaggtgat cgcgcccacg atgggcgaag accctcaggc cctcgggcgc
      661 ttcgagcgcg aagcgcgagc gctggcatca cttcatcatc caaacatcgt cgacgtatcg
      721 tcgttcggca cgctttccga cggccgaagt tacttcgtga tggagtacct cgtcggcgag
      781 acgctcgaag accgactcgt acgcgggcgc ataccgctcg acgaggcgct cgatactctc
      841 gatcagatgg cgcgtgcgct cgagggtgcg catacgcagg ggatcgttca ccgcgatcta
      901 aagccgtcga atacgttcct cgtacgaatt ccgcgcgagc agcgctcgat cgtgaagctt
      961 ctcgacttcg gactcgccaa gctggcagtt gcggacggcg tcgagaaaac cgcgagcggc
     1021 gcggtgatcg gcacggcgct ctatatttca cccgagcagg cccgcagtcc gaacgtcgac
     1081 ggtcgaacgg acgtctatgc gcttggctgc attgcgtacg agcttgtctt gaaccgccac
     1141 ccatttcccg aggcgcgaac cccaacggcg gcgattgcgg cgcacctgac ggaaccagtg
     1201 ccgcagccga gaacgatctg gcccgggatt ccggccgcgc tggatctgct gctgttctcg
     1261 atgctcgcca aggatccgag ctaccggccc acgctcgcgc aggtacgcaa cgtgatcgca
     1321 agcgtgagat cgccgacaac ggctgcaggt cgtgagatgc gaatgtcgac ggagccgacg
     1381 agacgcgtcg atctgcgctc acgcgtttgg atggcagcgt tgatcgcgtt cacgcttctc
     1441 gttgggatca tgatcggcgc ggtcgcgctc ggccggaaat cgagcaacgg taacgcgccg
     1501 cgagaagcgc cagccggcct cggcgccgca agccgcaaca gcgagaaccg agctacgccg
     1561 cccacgccct tggagccggc gccagtcgcg ccgagtccag tcgcgccgag tccagtcgcg
     1621 ccgagtccaa cggcgccgag ctcagtgatt gagacgtcgg ttgcaccaat tcccgctcca
     1681 gtagcggata cgccaaaagt cacaaagcca gcgggccgct ccatccctca ctcgccgcga
     1741 cagacagcgg cgagctcagc tgccagcgat ggggcgctcg cgttggactc caaaccgaaa
     1801 atggacgttc tcgtcgacgg cgagcccaag ggccaaacgc cggtcttcgg tctgaagctt
     1861 ccggttggtc gtcacaagat cacgctgatc aacgacacgt actccatcaa ggagtcgttt
     1921 cgcgtcgaca tcaagccggc ccgagtcgag agaatcgtca aggactacag cgatcgcatt
     1981 cccgaggtga agaaaccgga cgcccgcacg acgatcaacc cgtttgagaa acaaggctcg
     2041 gctgcgcgat gagctcgtga tcatttgtcc tggcgcgaat cacaccgttc tggcgacaga
     2101 acgagcgcct gcgcgcattc cggcattcag gctccaacgt cgagttgggc tccgggcaca
     2161 caaaggcccg cgagcgccga aacgcgatag ccatcgacga agactgggta caagtcattt
     2221 gagatccact agtgtgctgc atgctcgtga tcctgcatgc agggcaaacc ggcgttgaac
     2281 gcggtgccca tcgagctgcg acggcaaccg caatctttgt tgccggattc atgcccctcg
     2341 acaaacgaga cgagctgggc ccgattccgc gcgaaattgc tgaagtacta acaccatgcc
     2401 ctgaacgcgg ggtgcgttct gcggtgcgct cgaacatcaa gattgcatcc gccgttttgc
     2461 tagtcgtgcc ggatcaatca cgcgtcgaga gattcaccgc gatgaccgct gtgcttcagg
     2521 cgatccgcgc tgcacgcatt ccttcgttga tttgtgaccc tgcgacgaat ctcgcggatg
     2581 tcacgacctg gggaggtcga gtttgcagta cgtcggggtc caggagaatc ctggtgacag
     2641 gtccacgcga aacgagatgg atggatgggg agagcgtggc gcgtagaatg gtcacagcga
     2701 tcggcatgac gctcaaatga gtttcgtcat gctctcgcaa gcttcgcaac ctccggcaag
     2761 cgatgaacta agctgtgccg tcgtgccggt aatccagcgt tcgctcgcgc gctacctgct
     2821 cgtcggtagc gtgctggctg tcgtcgtcaa cgtggtgatc aacgctgccg tcggcaacgc
     2881 gctctatgcg catgtcgcga ccattccgct gaccggtgat ccgagcatcg caggcgatac
     2941 gatcgtgggc gcgttcctga tcgccttctt cacggtcgtc gtcgtcgcgc cggcgacgcg
     3001 ccgcgaaatc cgcagcggcc gcgtgcgcgg tggccgcaag ctcacgctgc ccggctggtt
     3061 cgctcgtcgt ccgctgctcg ccgccgtcgc gggcggcctc gtgtcggcga cgctgatcgg
     3121 cggcggcgcc gtcgcgatca ttgcggccat cggcgcggca ccgatgtcgg gccacgcctt
     3181 cctcgcgttc aagatcgcgt tcgcgggcgt ctggggtggc ctcgctgccg tgctcgtcgc
     3241 cgcgctcgcg gccgccggtg aacccgaacc tcccgtcgac gatcgctggt gccgtgaccc
     3301 agcggccccg gtcgccgtca gctatccgtg cgacttcttc gacaaaggcg ggctcgccgt
     3361 cacgagtgcg cgccacggct gctccggcac gccgacgtgg cagctcgtcg tgacgggcgt
     3421 gcccgatccc gcagcggttc gcgcggcgct cggcgatctg ctcgtgcgct atccagcgct
     3481 cgccacccgc gtgcagtcac tcgacgccat ccccgagtac gcgcgtcggt ttcgctacgc
     3541 tgcggcgccc accgacgtcg attccatctt cgagctcatc gatctgcgcg gccgcccgtc
     3601 gagcgagctc gaggccgtga tccaccgcgt gtggaaccgc cacctcgatc agttccgcga
     3661 cgccccgatc tcgctgacga tggcgatcac cgacgacgag cactgccgcc ttctgtttcg
     3721 ccagcatcac ggcatcgcgg acggtcgcgc cttcatcggc ctcctgaacg atttcgtcgc
     3781 cttcgtacgc gcgcgccaga gcggcgcggc accggctccc gccgcgctcg agccgatcgg
     3841 ccgccgcggc gagctcgacg ccctcggcct ctcgccgacg actcgcgcgc gctacgcgct
     3901 cgctggtttc gcatcgctga ccgcctcgat cgtcaaggcg atcgttcgtc cgtccgcgat
     3961 cctcgtgcag aacgagtcgg cggactacac cgggccaaac ggtgcggtcc gctggatcgt
     4021 cgacgacacc gtcctcgacg cctggcagcg tgcacgcaag cagctcggcg ttagccagag
     4081 cgccttgttc atggccgcgc tgttcgtcgc aacgcagcgc tggcaccgcg agctcggtcg
     4141 ccgactcggc cgtgtcaacg catcgctcgt gatggagacc cggccgcgcg atggcttctt
     4201 cgtgtcgttt gcgaaccacc tcgcgacgct cgaggtcgag ctacacctcg agcgcgacct
     4261 cgatccggcc gacgccgccc gcgcgatcca cgcgcagctc aagcgccagc tcgaccgcaa
     4321 ccggccgctc aaacgtctgg tgtgcgagcg agcccttgtc gcgggcatgc cgctcgacaa
     4381 gctccaggca ctggtcttcg acgcgaaacg gcctgccttc aacctcaacc tctcgaacct
     4441 gatcccactc gactttcccg tgctctccgg ccctgcatgg accgtcgacg aagtcctgat
     4501 cacgacgccg gtcacgcc
//
LOCUS       DASUFX010000568         8809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1341176, whole genome shotgun sequence.
ACCESSION   DASUFX010000568 DASUFX010000000
VERSION     DASUFX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341176"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFV99_22735"
     CDS             <1..599
                     /locus_tag="VFV99_22735"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX5062212.1"
                     /translation="RAVAVADYEVLARRAPGADIRRARAIGGLHPRFIGTRIPGVVGV
                     FVVGAARDDGQPPIPTEATLHAVSDYLSSWAPRGAEVVAVAPTFHSVRVEASFELAAR
                     VDVTETIHDVSRKVDRWFDPVNGADGEGWPFGGTIYADALIRFLLRELTGRGLAVPRL
                     LFVVVGVRSRHCVNVPIPEHDLLWPAPHELVALPRRTP"
     gene            596..2608
                     /locus_tag="VFV99_22740"
     CDS             596..2608
                     /locus_tag="VFV99_22740"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX5062213.1"
                     /translation="MTCGPEPVTFRILDHDAQWKLDLRPEPLKVTINGVIELAQLDPN
                     AIDPATLATLIPPPWLAHGCGACDWYLLCGSTVLRYGPRIIDCEPKEEEDRCAPPASA
                     DPSCLGWVAIAGAGCHIELVRPVAIAATRDRVAVLDIGRHELLVLSADGARVIASIPT
                     NARGPIAFWGRSIVVADHDELVAYDLLTLAPRALPNAPGPIARMIAAGDALWLAVAAG
                     NALHLFRIDHAGHIEPGTLDELRKTARPTGIASAADNVVCLEIPRGGGDPRIACIDRC
                     GRPVAPPPPQLPPERYREGWIKTAHDAPIDSGIPRCKWHRVRLDLDLPERTGITISLA
                     TVEDPSLPIADDDWQHIEDPKAIHDFLIDQPPGRFLMLRMQLRGDRYATPRIRRIRID
                     FPRSTSATLLPGIYREDPVSADFLDRFLATFDASIEDLDRIIARFPALLDPASTPAEA
                     LAWIGTFLDIALDPAWSEATRRAILGEAPELYRKRGTPWALARAVELAIGVSPAVQEL
                     GGSGTYGRVAKAGAKNGFRVGEARLFGAARARMRLGVSALGKAPLRSYGDPDRDHMAA
                     IGWRILVQVPRITETDALTRLRRLVDSQKPAHVAAQVRMGGELALVGVASAVGIDTLL
                     GGLPLPYLGRNTHLRRRSVLARGRARGGASMAVGAAGAVAIQTVLT"
     gene            2616..4010
                     /locus_tag="VFV99_22745"
     CDS             2616..4010
                     /locus_tag="VFV99_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062214.1"
                     /translation="MSKYVPAHSHDTHQHSRHTDYDEGCLPLPRFERVKYFYGQLLGV
                     REFQSEQSYFREKHRLHNRYLHGYGVVCGLGVSLCRHPPGPCDEPEPKHEPEKPTGEP
                     SGEPPGSPTQGKPTQQMQVQGSKALVHQPILVPPQMPPKPCIEVDCGLALDCQGNEIV
                     VPWPTQIDLIDALGCDAPKHLLEGKYAYVTLCYVEQPIEPVRPLTADNCGGLLPDCVP
                     SRLRDHFCVRITWEKPPHDVSCSPCARPCCEPCLPIARFWWSKEHGFVIDNGIRRTLA
                     PYVTTKVSAISWVHDAVYRAQDVDDMLRCGGITIHFSDDVRTASLRRGAFDIWIVQGG
                     GGRRGDIYNVPIEVEPSHPHQEFSRSVTLVMPSTHKDRGDRIDHDDRVLITMRSAFVL
                     DRCCRAVDGEHIGGFVPVTPDTEKRWAPVERPPVVPCRPPEPDPFTPWMSGNGVPAGS
                     FESWLYVSEEDKQY"
     gene            4021..5910
                     /locus_tag="VFV99_22750"
     CDS             4021..5910
                     /locus_tag="VFV99_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062215.1"
                     /translation="MQTTKPKTGCGCGGTSSGGCGCGGKSSGGCGCGGGCGCKPAKLP
                     EAAPALCDPCETASFIRPRFFAGQLLTEDDLGALIDYTVAKNRFHNSRLFGAGIVCGL
                     AVECGPCNGSQIIVQPGYALDCCGNDLVLTCKRTLDLVPMIRDLAARKELCTDPCPPP
                     QKAEETKGKNDDKQEVLPLKKYCLYARYAERDDQPVATYPLGDNCDAATCEPTRVIEG
                     IVFELRCPPPKKSKTVKDVMEDCAFWLDRENDLTGSAKALRVAAMALTIGTGRPTDDD
                     HDLLRELAKHGPEALTSGLEGPAKYEATLMLSSRAAALVGRMKAGELTIKGLEVEKLE
                     KDLKKMAETVSAAPEAAVHPLMIELGNAVPNYVELLGDKRHDVEAIDAIKAGPFGKTK
                     VLDTFGDNLERIAELLQREAGCGKSRIHTDCTLPALIDTLTPKWNRRNLDSLQYEKAA
                     LQFQDAACAIERYLYDCWCAAINPPCPPCDDPGVLLACITVDACNVVQICNTDREYVL
                     APSTLRYWDMLPRPDTSKCCDHTCRHRKPGIIVDPVHGGTIEQPTLTLMPREVYPFLT
                     RAMKNVAELQGMDAPSVPVAERAKIFAGAAPRSREAELIDELFKEVEALKAKIQKLEK
                     KKGES"
     gene            5907..7070
                     /locus_tag="VFV99_22755"
     CDS             5907..7070
                     /locus_tag="VFV99_22755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062216.1"
                     /translation="MSASPMLRPTFKEGQILGAADLNAQVEYERKGSVFHERTEHLWG
                     VAQGLTLDTTQQKTTDPTKPPTKYVDVTLAPGRAVDRLGRSILVTEGIPLDPDMFTQQ
                     IASPTDGVLYPVFVQAIEVARTGETQPGKCSVSLETRVEESLQIAFGSPGEEITVLDQ
                     AAVPVSEGFGTPALSDKVLVGWVKFNTTIKKFDSVATESSTTRIRYVGVVASDVVAGG
                     GVLTLHTRPEGARFAVAITENSLTGKCTLEFGKQDGNGPVVPVLTVDEKGNIEYKGTL
                     TPAPLSKTLAESGVISDGIKVPLPPEVDEDKVNQGTIRLHAHLTPIPQAPKNVLIGGA
                     LILAYPLVTRCVVDIAGDRVVHCQVRWYDPANPTTKFVDLPGLCSYTVFATGK"
     gene            7155..7658
                     /locus_tag="VFV99_22760"
     CDS             7155..7658
                     /locus_tag="VFV99_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062217.1"
                     /translation="MTKKEDADALLKKLIGDKLQIAIGDVGVLEISATRVAADLIDAK
                     QDFERVVSNVYGYRLKLGGNDKPGDGNDKVVEPAKSNFVTNIKFNGSPATEVQVDLKE
                     QEGPFGKDAFLVFEGLDPIKGKTTPNAKDAKFPLDPTLVLKSGEFYRMLFIKDDVPVS
                     AAFLKAP"
     gene            7793..>8809
                     /locus_tag="VFV99_22765"
     CDS             7793..>8809
                     /locus_tag="VFV99_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062218.1"
                     /translation="MAARIPGPVHVTEPVRVKIATTVRELVALGDGASPAAAALQAEV
                     ARKVAEALARIAPSSSNEVESREGAGAVGADSDEPDAMLPALDDAAAPWLALRLWWRS
                     RAIVDVLTRMDDRAIAFLEDLLFPPDVVAHSLRESAPPPASASDRRALIAIARAAASQ
                     IAFGDNTQAAAARTRIAIAAAIAESAPTATPRTIHAAIADVIEQLAPSQPETERTGRT
                     AVHRRPNGMVEVRSVLPFLLLPSLHHAGWLASARTLLAAHDVEDDAFALAAGIAARVL
                     DPLDRGWARSRADRLVIATFAGRDESLEDREIAAASTRLAPLLPALDAGLRAVLVRAR
                     RPRPL"
BASE COUNT         1571 a         2925 c         2850 g         1463 t
ORIGIN      
        1 atcgcgccgt cgcggttgcc gactacgaag tgctcgcgcg tcgcgcaccc ggcgccgata
       61 tccgtcgcgc gcgcgccatc ggcggtctgc acccgcgatt catcggcacg cgtatcccgg
      121 gcgtcgtcgg cgtgttcgtc gtcggtgccg cgcgcgacga cggccagcca ccgattccaa
      181 ccgaagcaac gctgcatgca gtgagcgatt acttgtcgag ctgggcaccg cgcggcgccg
      241 aggtcgtcgc agtcgctccc acgttccatt cggtgcgtgt cgaagcgagc ttcgagctcg
      301 cggcgcgggt cgacgtcacc gaaacgatcc acgacgtctc gcgtaaggtc gatcgctggt
      361 tcgacccggt gaacggtgcc gacggcgagg gctggccgtt cggcggcacg atctacgcgg
      421 acgcgctcat ccgcttcttg ctgcgcgagc tgaccggccg gggtctcgcc gtgccgcgcc
      481 tcttgttcgt cgtcgtcggt gtccgctcgc gccactgcgt aaacgtgccg attcccgagc
      541 acgatcttct gtggcccgcg ccgcacgagc tcgtcgccct accccggagg acgccatgac
      601 gtgcggtccc gaaccggtca cgttccgcat cctcgatcat gatgcgcagt ggaagctcga
      661 ccttcggccc gagccgctca aggtcacgat caatggcgtc atcgagctgg cacagctcga
      721 tccaaacgcc atcgatccag cgacactcgc gacgttgatc ccacccccgt ggctcgcgca
      781 cgggtgtggt gcttgcgact ggtacctgct ctgcggctcg acggtgctcc gctatggacc
      841 gcggatcatc gactgcgaac ccaaggagga ggaggatcgc tgcgcaccac ccgcgtctgc
      901 cgatccgtcg tgcctcggct gggtggcgat cgccggtgcc ggctgccata tcgagctcgt
      961 ccgaccggtc gcaatcgcag cgacacgcga ccgagtcgct gtcctcgata tcggccggca
     1021 cgagctcctc gtgctctccg ccgacggtgc acgcgtcatc gcttccattc cgacgaacgc
     1081 gcgcggaccg atcgcgttct ggggacgttc gatcgtcgtg gccgatcacg acgagctcgt
     1141 cgcgtacgac ctcctcacgc tcgcaccacg tgcgctacca aacgcgcctg gcccgatcgc
     1201 gcgcatgatc gccgccggcg atgcgctgtg gctcgccgtc gccgccggca acgcccttca
     1261 tctattccgc atcgatcacg ccgggcacat cgagcccggc acgctcgacg agctacggaa
     1321 gactgctcgc cccaccggaa tcgccagtgc cgccgacaac gtcgtgtgcc tcgagatacc
     1381 gcgcggcggc ggtgatccac ggatcgcctg tatcgatcgg tgcggtcgtc cggtcgcgcc
     1441 cccaccaccg cagctgccac cggagcgcta tcgcgagggt tggatcaaga cagcgcacga
     1501 cgctccgatc gacagcggca tcccccggtg caagtggcat cgcgtccggc tcgatctcga
     1561 tcttcccgag cgcaccggca tcacgatctc gctggcgacc gtcgaggacc cctcgctgcc
     1621 gatcgccgat gacgactggc agcacatcga ggatccaaag gcgatccacg atttcctgat
     1681 cgatcagccg cccggccgct ttctcatgct ccgcatgcag ctgcgcggcg atcgctatgc
     1741 aacgccgcgc atccgccgga tccggatcga ctttccgcga tcgacgagcg ccacgctgct
     1801 acccggcatc tatcgcgagg atccggtctc ggcggacttc ctcgatcggt tcctcgcgac
     1861 gttcgatgca tcgatcgagg atctcgatcg catcatcgcg cgctttcccg cgctactcga
     1921 tccggctagc accccggccg aagcgctcgc ctggatcggt acgttcctcg acatcgcact
     1981 cgaccccgcg tggtccgaag cgacacgtcg cgcgatcctc ggcgaggcgc ccgagcttta
     2041 tcgaaagcgc ggcacaccgt gggcacttgc gcgggcagtc gagctcgcga tcggtgtctc
     2101 gcccgcggtc caggagctcg gtggttctgg cacgtacggg cgcgtcgcaa aggccggcgc
     2161 gaagaacggt ttccgcgtcg gtgaagcgcg cctgttcgga gctgcacgcg cacgcatgcg
     2221 gctcggtgtg tccgcgctcg gcaaggcgcc actgcgcagc tatggcgatc cggatcgtga
     2281 tcacatggcc gcgattggct ggcgaatcct cgtccaggtt ccgcgcatta cggagacaga
     2341 cgcactcacg cggttgcggc ggctcgttga cagccagaag cccgcacacg tggcggcgca
     2401 agtgaggatg ggcggcgagc tcgcactcgt cggcgtcgcg agcgcagtgg gcatcgacac
     2461 gctactcggt gggctgccgc tgccctacct cggacgcaac acgcatctac gtcggcgctc
     2521 cgtgctcgcg cgagggcgcg cacgcggtgg cgcgagcatg gcggtcggcg ctgccggagc
     2581 agtggcgatt caaacggttc tgacgtgagg agatcatgag caagtacgtt cccgcgcatt
     2641 cgcacgacac acaccagcac tcgcggcaca ccgactacga cgagggctgc ctgccactgc
     2701 cgcgcttcga gcgcgtgaag tacttctacg gtcagctgct cggggtacgc gagttccagt
     2761 ccgagcagag ctacttccgt gagaagcacc ggctgcacaa ccgctacctg cacggctacg
     2821 gcgtcgtgtg tggtcttggt gtgagcctgt gccggcatcc gcccggtccg tgtgacgagc
     2881 cagagccgaa gcacgaaccg gagaagccga caggtgagcc atccggcgag ccgccgggtt
     2941 ccccgacgca aggcaagccg acgcagcaga tgcaggtgca aggctcgaaa gcactcgtgc
     3001 accaacccat cctcgtgccg ccacagatgc cgccaaagcc gtgtatcgag gtcgactgcg
     3061 gcctcgcgct cgactgtcag ggcaatgaga tcgtggtccc gtggccgacg cagatcgatc
     3121 tgatcgatgc gctcggctgc gatgccccga agcatctgct cgagggcaag tacgcgtacg
     3181 tcacgctctg ttacgtcgag cagccgatcg agccggtacg gccgctgacg gccgacaact
     3241 gcggcggtct gttgcccgat tgcgtaccaa gccgcctccg cgatcacttc tgcgttcgga
     3301 tcacgtggga gaaaccaccg cacgacgtct cgtgctcgcc atgcgcgcgg ccgtgctgcg
     3361 aaccgtgtct accgatcgcg cgcttctggt ggagcaaaga acacgggttc gtcatcgaca
     3421 acggcatccg ccgcacgctc gcgccctacg taacgacgaa ggtgagcgcg atcagctggg
     3481 ttcacgacgc cgtctatcgc gcgcaggacg tcgacgacat gctgcggtgt ggtggcatca
     3541 cgatccactt ctccgatgac gtgcgcactg cctcgctgcg gcgtggcgcg ttcgacatct
     3601 ggatcgtgca agggggcggt ggtcgtcgcg gcgatatcta caacgtaccg atcgaggtcg
     3661 aaccgagtca cccgcatcaa gagttctctc gctccgtgac gctcgtgatg ccctcgacgc
     3721 acaaggatcg cggcgatcgc atcgatcacg atgatcgggt gctgatcacg atgcgcagcg
     3781 cgttcgtgct cgatcgctgc tgtcgcgccg ttgacggcga acatatcggc ggcttcgttc
     3841 cggtgacacc ggataccgag aagcgatggg cgcccgtcga gcgtccgccg gtggttccct
     3901 gccgaccacc cgagcccgat ccgttcacgc cgtggatgtc gggcaatggt gtcccggccg
     3961 ggtcgttcga gagctggctg tacgtgagcg aggaagacaa gcagtactga ggagacgatg
     4021 atgcagacca caaaacccaa gacaggctgc ggatgcggcg gcaccagtag cggcggttgc
     4081 ggttgcggcg gaaagagctc gggcggctgc ggctgcggcg gtggctgcgg ttgcaagccg
     4141 gcgaagctac ccgaagcagc ccctgcgctt tgcgacccgt gcgagacggc atcgttcatt
     4201 cggccgcgct tctttgccgg ccagctgctc accgaggacg acctcggcgc gttgatcgat
     4261 tacaccgtcg caaagaaccg cttccacaac tcgcgcctgt tcggcgccgg gatcgtgtgc
     4321 ggtctcgcgg tcgagtgcgg cccgtgcaac ggctcgcaga tcatcgtgca gccgggctac
     4381 gcgctcgact gctgcggcaa cgatctcgtg ctcacgtgca agcgcacgct cgatctcgtc
     4441 ccgatgatcc gcgatctcgc tgcgcgcaaa gagctctgca ccgatccatg tccgccaccc
     4501 cagaaggcgg aggaaacaaa gggcaagaac gacgacaagc aagaggtgct tccgctgaag
     4561 aagtactgct tgtacgcgcg ctacgcggag cgcgacgatc agccggtcgc gacgtacccg
     4621 ctcggcgaca actgcgatgc cgcgacgtgc gaaccgacgc gcgtcatcga aggcatcgtg
     4681 ttcgagctgc gctgcccgcc accgaagaag tcgaagacgg tgaaggacgt gatggaggat
     4741 tgcgcgttct ggctggatcg cgagaacgat ctgaccggca gcgcgaaggc tctccgggtg
     4801 gcggcgatgg cgctgacgat cggcactggc cggccgaccg acgacgatca cgatctgctt
     4861 cgcgagcttg ccaagcacgg gcccgaggcc ctcacgagcg gcctcgaagg tcctgcgaag
     4921 tacgaagcaa cgctgatgct cagctcacgc gcagcagcgc tcgtcgggcg catgaaggcc
     4981 ggcgagctga cgatcaaggg gctcgaggtc gagaagctcg aaaaggacct caagaagatg
     5041 gcggagacag taagcgcggc gcccgaggct gccgtgcatc cgctgatgat cgagctcggc
     5101 aatgccgtgc cgaattacgt cgagctgctc ggcgacaagc gccacgacgt cgaggccatc
     5161 gacgcgatca aggccgggcc gttcggcaag acaaaggtac tggatacgtt cggcgacaat
     5221 ctcgagcgca tcgccgagct gctgcaacgt gaggccggtt gcggcaagtc gcgcatccac
     5281 actgattgca cgctgccggc tctgatcgac acgctcacac cgaagtggaa ccgccgaaat
     5341 ctcgattcgc tgcagtacga gaaggcagcg ctgcagttcc aggacgcggc gtgcgcgatc
     5401 gagcgctacc tgtacgactg ctggtgtgcg gcgatcaacc cgccctgccc gccgtgcgac
     5461 gaccccggcg tgctgctcgc gtgcatcacc gtcgatgcct gcaacgtggt ccagatctgc
     5521 aacacggacc gcgagtacgt gctggcaccc tcgacgttgc gctactggga catgctgccc
     5581 cgacccgata cgtcgaagtg ctgtgaccac acgtgtcggc atcgcaagcc gggaatcatc
     5641 gtcgacccag tacacggggg gaccatcgag cagccgacgc tgacactcat gccgcgcgaa
     5701 gtctatccgt tcctcacgag ggcgatgaag aacgtcgccg agttgcaggg catggatgct
     5761 ccgtcggtgc cggtcgccga gcgagcgaag atcttcgctg gcgctgcgcc gcgctcacgc
     5821 gaagccgagc tgatcgacga gttgttcaag gaggtggagg ccctcaaggc caagatccag
     5881 aagctcgaga aaaagaaggg tgagtcgtga gcgcctcgcc gatgctgcgc ccgacgttca
     5941 aggaaggaca gatccttggc gccgccgatc tcaacgcgca ggtggaatac gagcgtaaag
     6001 gttcggtgtt ccacgagcgc accgagcatc tgtggggcgt cgcgcaagga ttgacgctcg
     6061 atacgacgca acagaaaacg acagacccca ccaagcctcc gacgaagtac gtcgacgtga
     6121 cgctcgcgcc cggccgcgcc gtcgatcggt tgggacgctc gatcctcgtg accgagggga
     6181 ttccactcga tccagacatg ttcacgcagc agatcgcgag ccccacagac ggcgtgctct
     6241 acccggtgtt cgtgcaggcg atcgaggtgg cgcgcacggg cgaaacccaa cccggcaagt
     6301 gttccgtctc gctcgagacc cgcgtcgagg agagcctgca gattgcgttc ggctcgcccg
     6361 gcgaggaaat cacggtgctc gatcaggcgg cggtgccggt cagcgaaggc ttcggtacac
     6421 ccgcgctgtc cgacaaggtg cttgtcggct gggtgaagtt caatacgacg atcaagaagt
     6481 tcgactcggt tgcgaccgag tcgagcacga cacggattcg ctacgttggc gtcgtcgcga
     6541 gcgatgtcgt cgccggcggc ggtgtgctca cgctgcatac gcggccggaa ggtgcgcggt
     6601 ttgcggtcgc gatcaccgag aacagcttga ccggcaagtg cacgctcgag ttcggcaagc
     6661 aggacggtaa tggaccggtc gtgccggtgc tcaccgtcga cgagaaaggc aacatcgaat
     6721 acaagggcac gctgacaccg gcgccgctgt cgaagacgct cgccgagtca ggtgtcatct
     6781 ccgacggcat caaggtcccg ctgccgcccg aagttgacga ggacaaggtg aaccagggca
     6841 ccattcgcct gcacgctcac ctcacgccga ttccgcaggc gccgaagaat gtcttgatcg
     6901 gtggcgcatt aattctcgcg tatccacttg tcactcgctg cgtcgtcgac atcgccggtg
     6961 atcgcgtcgt gcattgccag gtgcgttggt acgacccggc gaatccgacg acgaagttcg
     7021 tcgaccttcc cggcctatgc agttacacgg tgttcgccac cggaaagtga ttcccgatgc
     7081 cctctgctct gtatgggatt tcgttcactg ataccggaaa cctgctggca gtctgcagct
     7141 tcgaaaagca gaccttgacc aagaaggaag acgcggacgc gctcttgaag aagctgattg
     7201 gcgacaagct ccagatcgcg atcggcgacg tcggcgtcct cgagatcagc gcaactcgag
     7261 tcgccgccga tctgatcgac gcgaagcagg acttcgaacg tgtcgtatcg aacgtctacg
     7321 gctatcggct gaagctcggt ggcaacgata agcccgggga cggcaacgac aaggtcgtcg
     7381 agccggcgaa gagcaatttc gtcaccaaca tcaagttcaa cgggtcgccc gctaccgaag
     7441 ttcaggtcga cctcaaggaa caagaagggc ccttcggcaa ggacgcattc ctggttttcg
     7501 aggggcttga tccgatcaag ggaaagacga cgcccaacgc gaaggacgcg aagttcccgc
     7561 tcgatccaac actcgtactc aagtccggtg aattttaccg gatgttgttc atcaaggacg
     7621 acgttccggt cagcgcggcg ttcctcaaag cgccatgacc gacgtcgtga tccaccgctg
     7681 cgtgctgcgc atccgacgaa ccgaaggttg gtcgtggggc gctgacccga acgcgctatt
     7741 ggcgtctgcc acacgcgcgt tgccgcggtt gatcgcgtcg catctcccgg acgtggccgc
     7801 gcgtatccct ggtcccgttc acgtcaccga gccagtacgc gtgaagatcg cgacgaccgt
     7861 acgcgagctt gtcgcgctcg gcgatggcgc ctcgcctgcc gcggctgcgc tgcaagccga
     7921 ggtcgcgaga aaggtcgcgg aggcgctcgc gcggatcgca ccgtcgtcgt cgaacgaggt
     7981 cgagtcgagg gaaggtgccg gagcggtggg cgcggacagc gacgagccgg acgcgatgct
     8041 gccagcgctc gacgacgcgg ctgcgccgtg gctcgcactg cgattgtggt ggcgaagccg
     8101 agcaatcgtc gacgtcctca cccgcatgga cgatcgcgcg atcgcgttct tggaggacct
     8161 actattcccg cctgatgtcg tcgcgcactc gctgcgtgaa tcagcacccc cgcctgcgag
     8221 tgcatccgat cgccgagcat tgatcgctat cgcccgcgcc gccgcgtcgc agatcgcatt
     8281 tggcgacaac acacaggctg cggcggctcg cacgcggatc gcgatcgcag ccgccatcgc
     8341 cgagtcggcg ccgacagcaa cgccacgaac gattcacgcc gcgatcgccg acgtgatcga
     8401 acagctcgcg ccatcgcagc cggaaaccga gcgcaccggc cgaaccgccg tgcaccggcg
     8461 tccgaacggc atggtggaag tgcgctcggt gctgccgttc ctgctgctgc cgtcgttgca
     8521 ccacgcgggc tggcttgcga gcgcacgcac gctgctcgcc gcgcacgacg tcgaagacga
     8581 tgcgttcgca ctcgcggcgg gcatcgccgc gcgggtgctc gatccactcg atcgcggttg
     8641 ggcacggagt cgagcggacc gactcgtgat cgcgacgttt gcgggccgcg acgagtcgct
     8701 cgaagatcgc gagatcgccg ccgcgtcgac gcggctcgca ccgctcttgc ccgcactcga
     8761 cgcgggacta cgagcggtgc tcgtgcgtgc ccggcgccca cgcccgctc
//
LOCUS       DASUFX010000569        19079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_231426,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000569 DASUFX010000000
VERSION     DASUFX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19079
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231426"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFV99_22770"
     CDS             complement(<1..453)
                     /locus_tag="VFV99_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX5062219.1"
                     /translation="MGERDLQRNRAGTPADSGSTEEPETTNRTQFRSRGAIPPVEKSG
                     GTAQRSASDRAFGEDQRTHQLAEQGVSGNGQQLPHADRIAQSFGGAHADTVHGIQAHV
                     GGPAAQATGALGAQAYAHGNAVAFGASPDLHTAAHEAAHVVQQRAGVHL"
     gene            complement(569..1405)
                     /locus_tag="VFV99_22775"
     CDS             complement(569..1405)
                     /locus_tag="VFV99_22775"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5062220.1"
                     /translation="MTLRYGAVLSSLQSLRGAPGVPIVTPTHADVRYRDRQSPRGAIA
                     PLADLYVPRVPNGASVVLVHGGGFVLGSRRMKPMRYLTAKLVEAGIAVCVFDYRMIFR
                     GGRLDEATADVVDAFEFWAARTTALGLDPARISLAGLSAGATLAMLAAGQLDDGKLAN
                     LACAFGLYDADHLRGPAQLLPRLLVKTADRSGWNARFPRHARQPAAPTLLLHGSDDGL
                     VPVDQAHQLAAHRETLGLPTRLVIYDGAPHGFFNLPVPEAAAGAREIIDHVTRRRASS
                     CE"
     gene            complement(1402..2061)
                     /locus_tag="VFV99_22780"
     CDS             complement(1402..2061)
                     /locus_tag="VFV99_22780"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5062221.1"
                     /translation="MKKKTAKAAPDLSHERIRSAALELIDKDGLEEFSTRKLGRLLDC
                     EAMAIYWYYPSKDALLDAVVDKLMLGVGARLAETKTDDWIGTMRGLAHAYRQVAHDHP
                     KAFPLLATRRFASEGTFDFLDRLFTLSREQGIDDKVTARFYRVISSYCSGFALSELAG
                     PRGPQDPRTAPLRKKYPRVAAVSEWLDSKRLDEIFEFGLEVQLAALDNAVRSAHKGHT
                     R"
     gene            complement(2067..2594)
                     /locus_tag="VFV99_22785"
     CDS             complement(2067..2594)
                     /locus_tag="VFV99_22785"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5062222.1"
                     /translation="MIVAIVLGVFTWSLLEYLIHRFLGHRFNRNPFGVEHVRHHIEGN
                     YFAPSWKKLVAASIFAAVVIGPAILVAGTTHGIAYVAGLVTFYLTYEWLHRREHTHAG
                     IGPYGRWARRHHFHHHFVDGRTNHGVTSPIWDFVFRTYEKPAVIRVPAKLCMAWLLDP
                     SGTIRAEHAHTYQLR"
     gene            complement(2591..4345)
                     /locus_tag="VFV99_22790"
     CDS             complement(2591..4345)
                     /locus_tag="VFV99_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5062223.1"
                     /translation="MFDLKLTGGTLVDGTGRAPYAADVAVQDGKIVEVGACSGPARRT
                     IDATGAHVMPGFVDIHTHYDGQVSWDPDLSPSSLLGVTTCVMGSCGVGFAPVRPGRES
                     ALVELMEGVEDIPGSALAEGIPWGWESFADYMAAIAAMPHAIDVLAQVPHDALRMYVM
                     GERAVAGEVATDTDISEMQRLLRAALDAGAAGFSTGRSDNHRTARGAATPASEVDERE
                     LVGLAAAFRGLRHGVLQAVSDFDMMRGPEHFDGEFDLLEKMAEASGGRPLSISTMQRD
                     HAPDQWRQIFARVDRAVERGLDMRCQVGARGIGVLLGLEATFHPFMGFPSYKAIAHLP
                     LAERVARMRNSELRARMLAETSDKVAGDNTPIPALADFFLANINKVAFRIYRMTERPD
                     YEPDLQRCVAAEAARANQPVLARIYDALLEDDGKALLYFPVYNYTGMNLDIVREMLMH
                     PRALVGLGDAGAHVGTVCDASMPTFLLTHWARDRKHGIALERVVQMQARDTARFIGLA
                     DRGTVEVGMRADLNVVDMSRLSLARPVMQRDLPAGGRRLVQRADGYLATFVAGVALAE
                     HGALTGARPGQLVRMGVA"
     gene            4397..5707
                     /locus_tag="VFV99_22795"
     CDS             4397..5707
                     /locus_tag="VFV99_22795"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5062224.1"
                     /translation="MRAVLLTLTALLSLAATTPAQAGGFGIPDIGVRRTAMGAVIGRP
                     DEAAAIYHNPAGLVLQHGWHLYASLGLAFVRSEFQLHSWDQSDRFLGVNADADGYYQP
                     ARPTRALGAIPMLAVTGEILPGKLVLGTALYVGNAQGAGFDENGVTRYHLIDGYVVAP
                     QAVVAASYQLLPTLALGASAGVVNVRIHGRREVFPIVDGADISSIVGTRPELVLDGSG
                     WAPSWMVAAFGRPHPRVTWGATITGRIDATMEGPVTITYSDDATTPGDTLKGTQKTTQ
                     LLPWAFMAGANVDVTPQIEVGAEARYWLYRQYKKQHTDVVGIFLVRELETMKNYNDSW
                     QVSGGVRVHDLAAAPGLEMMLGTHFDRTPAPSSTVTFDQPTFRHIGLHSGARYSVGRY
                     RFGASYLRYFYLIPTVTDSTTTPPSNFRGHGANHIFTASVEVAL"
     gene            5770..6831
                     /locus_tag="VFV99_22800"
     CDS             5770..6831
                     /locus_tag="VFV99_22800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5062225.1"
                     /translation="MTGLRVIISQLGDEPLQAIDQFATLQPMDPPDPATLGTRDVVVR
                     VASASVGWVDLLMSTGQYQHVPAPPYTPGLEYAGTVHWAGAEANVAVGARVLVDPFLA
                     GPRSLGAYRAYGGFATYAVAPVEAILPMPGALSFDQACNLLGNYETAYHCLVARGRLA
                     AGETVLVHGASGSVGLAAVHLAKLLGATVIATGRKADKLDVVRTQGADHVVALRDGWR
                     DEVKALTDGRGVDVVYDGVGGEVSVESLRCVRFGARYLIVGWAATPFAARGVNSPNQL
                     PTNLIMMKGLDVLGCPTAIAATKDPSIRRERLAQVLAWANAGQITPLVARVYPLAEFA
                     AAMRAKWYGELVGGLVLHP"
     gene            complement(7095..7337)
                     /locus_tag="VFV99_22805"
     CDS             complement(7095..7337)
                     /locus_tag="VFV99_22805"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5062226.1"
                     /translation="MHAKTMLIDGKLAVIGSYNLDPRSAASNSEALVTVRDGTAVTEL
                     ATELQAMLGYSDRASADISAADWVKAKSFKIAEPLL"
     gene            7546..9099
                     /locus_tag="VFV99_22810"
     CDS             7546..9099
                     /locus_tag="VFV99_22810"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX5062227.1"
                     /translation="MTKTRGFLMLVVAACGGSSSSGDDTPPDASARCVPVAGAAAETV
                     MTTSGLVHGAVANETFAYKRVPFAAPPMGELRYAPPQPPACASGEIDATALGPVCPQL
                     DNGAFVGDEDCLHLNIWTPSAPAGAPRPVMVWIHGGGNSIGSAVYPIYDGRRLAEVGD
                     AIVVTTNYRLGQLGFLALGGDGAGVYGMLDQIAALQWVRDNIAAFGGDPSNVTIFGES
                     AGARDVCSLVASPAAKGLFHRAIMESGSCKALPTRTAAEQTAAQVATTVGCAAGDVAC
                     FRALPAETIIRANASDISGLSSGAYQPMIDGVVQTDQPDNVIAAGAHSHVPFVVGANA
                     DETGNSAPAIMTEAQYDALVHSQLPMAIADMALQQYPASAYPTPRAAYVRLTTDARFV
                     CPAREIARAADAGQSDAVYRYFFQYRASPLGAVHGLDVPYVFGTFDGIETPNGNPYTP
                     TATDLALSASIQGYWTRFARTGDPGGTPAWPLYGAADPTLVFDATVSTTDGIRTAHCD
                     FWQPFYDAI"
     gene            complement(9096..9887)
                     /locus_tag="VFV99_22815"
     CDS             complement(9096..9887)
                     /locus_tag="VFV99_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062228.1"
                     /translation="MRITIVPLAALLIPAAAYANPPADDPPTLHVDPSLDECEVHFAP
                     ELTQSAYHRFAREFGSVSAFKQMGAPQVVGTKGVSFGIEYMAFRIDERADAWNDTFSH
                     PTADHWLGSDKQFPKLKLRVGVGSNTDIGAYYTMNPMSNFGWIGVDVKHALLRQDEQK
                     PVTVAVRGAYTKTLFVDDMDMHALTGDVSVGRTLRYQITPYVGVGADGVLARETSSMV
                     ELDTEYSLVGHAFAGFEVAYRSLRFGAEGQFSAVSSAQAQLAWVF"
     gene            10118..10879
                     /locus_tag="VFV99_22820"
     CDS             10118..10879
                     /locus_tag="VFV99_22820"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HEX5062229.1"
                     /translation="MTDDGEVEDDWDFYPCQVDDAVASIFLNLRFEHATPPLAANTLF
                     RIRVQMLEPDEHGMGTTQEASVLNEIEDAMAERAAESGLIYVGRIRSGGVWEVAFYGA
                     PECKDALQAMRALFPDRRTYLDVRPDPEWGFYREFLLPDEERRQWMQDRRVTDALAER
                     GDSLTAPRRIDHWAYFTTAEGRDQFVGDVKAAGFALERAAHVEGHELPFAAQFYRDDA
                     AELEHIHDVVMLLFELAEKHDGDYDGWESPVQEAS"
     gene            complement(10881..11360)
                     /locus_tag="VFV99_22825"
     CDS             complement(10881..11360)
                     /locus_tag="VFV99_22825"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5062230.1"
                     /translation="MRARGNALAARLVAAYMDLAHAHDRFATRLLATVAVLALTITFA
                     TRVRRDAPYMMVDELVTKRLSDSFGHGIRVHGWIRAGSIDHAGDDHRFVLTAKSAELR
                     VHYRGEWPARIRDQSEVIMRGTLTFDDGGWLLEANEMMWRCSIKYGDEDRRLDTVYK"
     gene            complement(11461..12369)
                     /locus_tag="VFV99_22830"
     CDS             complement(11461..12369)
                     /locus_tag="VFV99_22830"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HEX5062231.1"
                     /translation="MRFSVGICVFVIACSGPAGAPDPSDPGGGGGGGSSGGTLDAGSG
                     PPPAHEVMVTAIPELPGSRLFVRPDAIPAPGILMLHGSEGGSAPYIDDDARPLAEAGF
                     AVVTFCWFGCSGRPNRIYHIPLEDTLAGLAWLRASPAVGGRHVGLYGVSRGAEQAVLL
                     SSLAPTMMDTVGVHAPSDTIVAAYDPATEDAVYEYDQASGQYKMAAAWTWQGTQLYGE
                     RTEFGQAGPRIRVEDYTGELYLSHGVADTLWPVQRSRNIKAARDAAGRATEAHFWDGQ
                     DHILMGAAAQQFITTLTAYYHRTLDP"
     gene            complement(12565..12864)
                     /locus_tag="VFV99_22835"
     CDS             complement(12565..12864)
                     /locus_tag="VFV99_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062232.1"
                     /translation="MGIAKFAGTVVDCRTRHEVKKSMTAFLTHFDANWILTLAIDAAD
                     PRLPVKVGGETSLAIHSPVQLLFDAADECTGNRYELAVEIDDATGRPRWETLQRA"
     gene            12993..14246
                     /locus_tag="VFV99_22840"
     CDS             12993..14246
                     /locus_tag="VFV99_22840"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5062233.1"
                     /translation="MGGIVRIAIVVAMVACAANDAQTPLPPTVLSSPGPLILGHAELD
                     AKGACNSCHVDNTAALDANKCAACHRELSQRMAKNRGFHASATVHGKPCETCHVDHRG
                     RGYDPMGWKSLKGGRDGFDHALTGWPLDGAHVTAACRDCHSERDTAGLETYLGTDTLC
                     GTCHGSPHNFTKPALEACDRCHSEAAWKPALPRPQFNHDNRLDARMPLLGAHASVACR
                     ECHANGLFNLGVAQPDRCETCHDSPHAGGLYAKKDCMWCHSPTFKSFDKTFFDHTEHT
                     RFDIGGHKDLPCARCHPASLGAQKPAMACETCHAARSPHAKRFAQYGSPPACAKCHST
                     SFGTPPRAPWRPKGFDHTKNTVFPLVGKHAELACRACHTGSGPTSFEKLDIKKGCMGC
                     HTHEAVHDKKFKNEQCLLCHVSAGH"
     gene            14465..16882
                     /locus_tag="VFV99_22845"
     CDS             14465..16882
                     /locus_tag="VFV99_22845"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062234.1"
                     /translation="MDRRDNRVSALRLATTAAVVLVACHLGARFGTWLRFPEIGSALL
                     FPPYAIVTAALCRLPPRHWWIVLLAAGLGDIGPHLRGGASVTFALCAELLNHSRAVLA
                     AIGIRRFAGKIETLREMVAYMIIAVAVAPALAAIGGAVLVTTLGTSDDFLLAWQQWWL
                     SNAITAVTLLPVLLLDFRKLAAFSPRRLVETTLYVVVLVVGTGWTLEYVHPTHMYWAL
                     PFLLLAAARFGAHVTSAVLLAVTMLSIWGAVGRSDGFVTSSESLLELQVFLIDVAIPL
                     LLLAVLIDQQRRTASELTLSRRQIQSIVEDQTEMICRFRPDGTCTFANRAYCEAFAAS
                     DPVGRSVWQLAPTGVHHTALELQQLTRASPLATHEVSVPTTDRALRWQQWQERGLFDE
                     RGAIVEYQAVGRDITDRKRAEDEHAELEAQRSVEAALREADRRKDEFLAMLGHELRNP
                     LAPLGIAVKVLVEAPPGSSEAAWARGSIERQLGKMTRLIDDLLDLSRVTLGKIQLQLD
                     AVNLATVIESAVEETRPLINSLGHQLIVTLPDAPVWLRADAVRLTQVVANLLHNAAKY
                     TERGGRIEVTVTRDDAGVCIAVRDNGIGLDPACLETIFGLFSQLPDARERAQGGLGIG
                     LTLVRRLVELHGGSVVARSEGRNCGSEMVVHLPIAAQDALAPSRPVTPQVTPRASLRI
                     LAVDDNVDIAKGLAAVLGLWGHTVQTAHDGEAALAIATAFAPDVVLLDLGLPSINGVE
                     VAHRLQTSEYAPTLLVSMSGFGNDYVRQRAAENGFDYHIVKPIDLDWLRTLLDKCAHD
                     KPRAPTA"
     gene            complement(16879..18882)
                     /locus_tag="VFV99_22850"
     CDS             complement(16879..18882)
                     /locus_tag="VFV99_22850"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062235.1"
                     /translation="MSEPNRDLEQLDLTERAPPSFDRDELGLDAPADGESKPRATPPP
                     IPAAARTSRSMRAASVPPPIPDAARTSRPMRAASVPPPIPQRALTQSGRTLVPKTEEA
                     VDVDLTSFAPTEAPPSDQALERDPAPLDIAPRGTAPIPATRDAAVIDVPSLALADDWS
                     ISGTVEPASDWSLGNNAEPASLAAAASTTELASDAATDGAESPAEEEAPAVAPPLPTF
                     MLSAARTSLPTPPPLARDARRGTLPPPIRSSTSSFVVAPAPTPPATSSVLPAVSAFPV
                     VVEAKAPARHEDPTPLPRPHVEHTDEIWFDSMASTDSREADADELLWDSTSNLHRKVA
                     PIPVFKLGIAAVLVGGLSAMTVILLRGDHDAPARRTHHAAIAQEQTLAPTPAPVVTPA
                     APAVAPAAPAVAPVATPEQAGPAAVIPEPAPAATTPEPAPVANAPAAEPQVAAAEPPP
                     ASPTTRELPFTTEPEGATVTLITNGATSVVGVTPLNVNIDPALTYDIVLTRPGYRTRL
                     ARIDPKADEVHVVLVSNDEPAPARAKATHEEREERAETSTPAPPTRVNRTEAVASTPA
                     PTERAKRPEQRPSLAAPRPTRVAAETPKGGTGILMVSSKPPCEIAVDGVATMLMTPQR
                     AIKLKPGKHKVTLFNTARKIDKTFDVAILPGQPTKLIRDFMVH"
BASE COUNT         3176 a         6290 c         6354 g         3259 t
ORIGIN      
        1 gaggtgcaca ccggctcgtt gctggacgac gtgcgctgct tcgtgcgcgg ccgtatgaag
       61 gtccggactc gcgccgaatg cgaccgcgtt gccatgcgcg tacgcctgcg cgccgagcgc
      121 acccgtcgcc tgcgctgcgg gaccgccgac atgcgcctgg atgccgtgca ccgtgtcggc
      181 gtgcgcgccg ccaaagcttt gcgcgattcg atcggcatgc ggcagctgtt ggccgttccc
      241 gctcacgccc tgctcggcta gctgatgggt gcgctggtct tcaccgaatg cgcgatcgga
      301 tgcgctccgt tgcgcggtgc cacctgactt ctcgaccggt gggatcgcgc ctcgcgaccg
      361 gaactgcgtg cgattggtgg tctcgggctc ctcggtactt ccggagtccg cgggggtacc
      421 agcgcggttt cgctggaggt cacgttcacc catggtcctc attttacgtc ggacctacat
      481 ccatacttcc tgcccgcggg tcccgaaaca ccgcagagtt gtaaggcttt ccgccgcgtc
      541 gcgcagatcg tagccgactc gaccagctct actcgcagct cgatgcacgc ctgcgcgtca
      601 cgtgatcgat gatctcgcga gcaccagccg cagcctcggg aaccggcaaa ttgaagaagc
      661 cgtgcggcgc gccgtcgtag atcaccagtc gcgtcggtag tccgagcgtc tcgcggtgcg
      721 ctgcgagctg gtgcgcttgg tcgacgggga cgagcccgtc gtccgagccg tggagcagca
      781 gcgtcggcgc ggccggctgg cgcgcgtggc gaggaaaccg cgcgttccag ccactgcgat
      841 cggcggtctt caccaggaga cgcggcagca gctgcgccgg gccgcgcaga tgatcggcgt
      901 cgtagagccc gaatgcgcac gcgagatttg ccagcttgcc gtcgtcgagt tgccccgcgg
      961 cgagcattgc caatgtcgcg ccggcggaga ggccggccag cgagatgcga gctggatcga
     1021 ggccgagcgc ggtggttcgc gctgcccaga actcgaatgc gtcgacgaca tccgccgtcg
     1081 cctcgtcgag ccgcccgccg cggaagatca tccggtaatc gaagacgcag accgcgatgc
     1141 cagcctcgac gagctttgcg gtgagatagc gcatcggctt catgcggcgc gagccgagca
     1201 cgaacccgcc gccgtgaacg agcacgaccg atgcgccatt gggaacgcgc ggcacgtaca
     1261 gatccgcgag cggcgcgatc gctccgcgcg gcgactggcg gtcgcgatac cgcacgtctg
     1321 cgtgggtcgg cgtgacgatc ggaacgccgg gtgcgccgcg cagcgactgc agcgacgaca
     1381 gcacggcgcc gtagcgcagc gtcatcgcgt gtgtcctttg tgcgccgagc gcactgcatt
     1441 gtcgagtgcg gcgagctgga cctcgagacc gaactcgaag atctcgtcga gacgcttgga
     1501 gtcgagccac tcggagacgg cagcgacgcg cgggtacttc ttgcgcagcg gtgccgtgcg
     1561 cggatcctgt ggtccccgcg gcccggcgag ctcactgagc gcgaagccgc tgcagtacga
     1621 gctgatgacg cgatagaaac gtgcggtcac cttgtcgtcg atgccctgct cgcgtgagag
     1681 cgtgaacagg cgatcgagaa agtcgaacgt gccttcactc gcgaagcgcc gcgtcgcgag
     1741 cagcgggaat gccttcgggt gatcgtgagc gacctggcga taggcgtgag cgagaccgcg
     1801 catcgtgccg atccagtcgt cggtcttggt ttcggcgagc cgcgcaccga cgccgagcat
     1861 cagcttgtcg acgacggcgt cgagtagtgc gtccttgctc gggtagtacc agtagatcgc
     1921 cattgcctcg cagtcgagga ggcggccgag cttgcgcgtg ctgaattcct cgaggccgtc
     1981 tttgtcgatc agctcgagtg cggcagaccg gatccgctcg tgggacagat caggcgcggc
     2041 cttcgcggtc ttcttcttca ccgccgtcat cgcaattgat acgtgtgcgc gtgttcggcg
     2101 cggatggtgc cggacggatc gagaagccag gccatgcaca gcttggccgg cacgcggatc
     2161 accgccggtt tctcgtatgt gcggaacacg aagtcccaga tcgggctcgt gacgccatgg
     2221 ttcgtgcgcc cgtcgacgaa gtgatggtgg aagtgatggc gacgcgccca ccgcccatac
     2281 ggcccgatcc ccgcgtgcgt gtgctcgcga cggtgcagcc attcgtatgt gagatagaac
     2341 gtcacgagcc cagcgacgta cgcgatgcca tgcgtcgtac ccgcgacgag gatcgccggt
     2401 ccgatcacaa cggccgcgaa gatcgatgcg gcgactaact tcttccacga gggcgcgaag
     2461 tagttgccct cgatgtgatg gcgaacgtgc tcgacaccga atgggttgcg gttgaaccgg
     2521 tgtccgagga agcggtgaat caggtactcg aggagcgacc aggtaaagac accgagcacg
     2581 atggcgacga tcatgcgacc cccatgcgca cgagttgccc cggtcgtgct ccggtaagag
     2641 ctccgtgctc ggcgagcgcg acacccgcga cgaacgtcgc gaggtaaccg tcggcacgct
     2701 ggacgagccg gcgtccgccc gcgggcagat cacgctgcat cacgggccgc gcgagcgaaa
     2761 gacgcgacat gtcgaccacg ttgaggtccg cgcgcatccc gacctcgacg gtcccgcgat
     2821 cggcgagccc aatgaaccgc gccgtatcgc gcgcctgcat ctggacgacg cgctcgagcg
     2881 cgatgccgtg cttgcgatcg cgagcccagt gcgtgagcag gaacgttggc atgctcgcat
     2941 cgcagaccgt gccgacatgc gcgcccgcgt cgccgagccc gacgagcgca cgcggatgca
     3001 tcagcatctc gcgcacgatg tcgaggttca tgcccgtgta gttgtagacc ggaaagtaga
     3061 gcagcgcttt gccatcgtcc tcgagcagtg cgtcgtagat gcgtgcgagt accggctggt
     3121 ttgctcgtgc ggcttcggct gcgacgcagc gctgtagatc tggctcgtaa tcggggcgct
     3181 ccgtcatccg gtagatgcga aacgcgacct tgttgatgtt cgcgaggaag aagtcggcga
     3241 gcgcggggat cggcgtgttg tcgccggcga ccttgtcact cgtctcggca agcatgcgag
     3301 cgcgaagctc ggagttgcgc atgcgtgcga cgcgctcggc gagcggcaga tgcgcgatcg
     3361 ccttgtacga cgggaacccc atgaacggat gaaatgtcgc ctcgagcccg agcagcacac
     3421 cgatgccacg agcgccgacc tggcagcgca tatcgaggcc gcgctcgacc gcgcgatcga
     3481 cgcgcgcgaa gatctgccgc cactgatcgg gcgcgtgatc gcgctgcatc gtcgagatcg
     3541 agagcggccg accgccgctc gcctccgcca tcttctccag cagatcgaac tcgccgtcga
     3601 agtgctcggg gccgcgcatc atgtcgaagt cgctgaccgc ttgcagcaca ccgtggcgca
     3661 gcccgcgaaa ggccgcggcg agcccgacaa gctcgcgctc atcgacttcc gatgccggcg
     3721 tcgctgcgcc gcgtgcggtg cggtgattgt cgctccggcc ggtcgagaac cctgcggctc
     3781 cggcatcgag cgcagcgcgt agcaggcgct gcatctcgga aatgtcggta tcggtcgcga
     3841 cctcgccagc gaccgcgcgc tcgcccatca cgtacatgcg cagcgcgtcg tgcggcactt
     3901 gcgcgagcac gtcgatcgcg tgcggcatcg cagcgatggc cgccatgtag tcggcgaacg
     3961 actcccagcc ccacggaatc ccctcggcaa gcgccgagcc cgggatgtcc tcgacgccct
     4021 ccatgagctc gacgagcgcc gactcgcggc caggccgcac tggcgcgaag ccgacgccgc
     4081 agctgcccat cacgcacgtg gtgacgccga gcagcgagct cggtgacagg tccggatccc
     4141 acgacacttg gccgtcgtag tgggtgtgga tgtcgacaaa gccgggcatc acgtgcgcgc
     4201 ctgtcgcgtc gatcgttcgg cgtgccggac ccgagcacgc gccgacctcg acgatcttgc
     4261 catcctggac ggcgacgtcg gccgcgtacg gcgcacggcc ggtaccgtcg acgagtgtgc
     4321 cgccagttag cttgaggtcg aacacggact cacttttaca ctgtaaaata atcccgatca
     4381 agactattcg taagacatgc gcgccgtact gctcacgctc actgccctgc tctcgcttgc
     4441 ggccacaacg cctgcgcagg cgggcggatt cggcatcccg gacatcggcg tgcgacgcac
     4501 cgcgatgggc gccgtgatcg gtcggcccga cgaagcggcg gccatctatc acaacccggc
     4561 cggcctcgtc ctgcagcacg gctggcacct ctatgcgtcg ctcggtctcg cgttcgtgcg
     4621 cagcgagttc cagcttcact cgtgggatca gagcgatcga ttcctcggcg tcaatgccga
     4681 tgccgatggc tattaccagc cggcacggcc gacgcgtgcg ctcggcgcca tcccgatgct
     4741 cgcagtgacc ggcgagatcc tgcctggaaa gctggtgctc ggcaccgcgc tctacgtcgg
     4801 caatgcgcaa ggtgccggct tcgacgagaa cggcgtcact cgctatcacc tgatcgatgg
     4861 ctacgtcgtc gcaccacagg cggtcgtcgc ggcatcgtac cagctactgc caacgctggc
     4921 actcggcgcg tcagcaggcg tggtgaatgt ccgcattcac ggacggcgcg aggtgttccc
     4981 gatcgtcgat ggggccgaca tcagcagcat cgtcggcacg cgccccgagc tcgtgctcga
     5041 tggcagcgga tgggcgccat catggatggt cgccgcgttc ggccgaccgc acccgcgcgt
     5101 cacctgggga gccacgatca cgggccgcat cgacgcgacg atggaaggtc ccgtcacgat
     5161 cacgtacagc gacgatgcga cgacgccggg cgacacgctc aaagggacgc agaagacgac
     5221 ccagctgttg ccatgggcgt tcatggccgg cgcgaacgtc gatgtcacgc cgcagatcga
     5281 ggtcggcgcc gaagctcgct actggctcta ccgtcagtac aagaagcagc acaccgacgt
     5341 cgtcggcatc ttcctcgtgc gcgagctcga gacgatgaag aactacaacg actcgtggca
     5401 ggtgtcgggc ggcgttcgcg ttcacgatct cgccgcggcg ccgggcctcg agatgatgct
     5461 cggcactcac ttcgatcgca cgccggcacc gtcctcgacc gtcacgttcg atcaaccgac
     5521 gttccgccac atcggactgc acagtggcgc gcggtattcc gtcgggcgtt accgcttcgg
     5581 cgcgagctac ctccgctact tctatttgat cccgaccgtc accgactcga cgacgacgcc
     5641 gccgtccaac tttcgcgggc acggcgcgaa tcacatcttc acggcctcgg tcgaggtcgc
     5701 actctgaaac tcggtcgatc tgcgctggcc ccgataagtg aagcgcactg tcggtggcaa
     5761 gatgacgccg tgacaggact tcgggtcatc atttcgcagc tcggcgacga gccgctgcaa
     5821 gcgatcgatc agtttgcgac gctgcagccg atggacccgc ccgatccagc gacgctcggc
     5881 acgcgtgatg tcgtcgttcg cgtcgcgagc gcatccgtcg gctgggtcga tctattgatg
     5941 agcaccgggc agtaccagca cgtcccggcc ccgccctata cgccggggct cgagtacgcc
     6001 ggcaccgtgc attgggccgg cgcggaggcg aacgtcgccg tcggcgcgcg cgtgctcgtc
     6061 gatccgttcc tcgcggggcc gcgctcgctc ggcgcgtatc gtgcgtacgg cgggttcgcg
     6121 acgtatgcgg tcgcacctgt cgaagcgatc ttgccgatgc ccggcgcgct gagcttcgac
     6181 caggcctgca atctgctcgg caactacgag accgcgtatc actgcctcgt cgcgcgcggt
     6241 cgcctcgccg cgggagaaac cgtgctcgtg catggcgcgt ctggctcggt cgggctcgcc
     6301 gcggttcatc tcgcgaagct cctcggtgcg accgtgatcg caaccggtcg caaggcggac
     6361 aagctcgacg tcgtgcgcac acagggcgcg gatcacgtgg tcgcgttgcg tgacggctgg
     6421 cgcgacgagg tcaaggcgct gaccgacggt cgcggggtgg acgtcgtgta cgacggcgtc
     6481 gggggcgagg tgtccgtcga gagcctgcgg tgcgtgcggt tcggtgcgcg ctatctgatc
     6541 gttggttggg cggccacgcc gttcgcagcc cgcggtgtta attcgccgaa ccagctgccg
     6601 acgaatctca tcatgatgaa gggtctcgat gtgctcgggt gcccgacggc gatcgcggcg
     6661 acgaaggatc cgtcgattcg gcgcgagcga ctcgcacagg tgctcgcgtg ggccaacgcg
     6721 ggtcagatca cgccgctcgt tgcacgcgtg taccccctcg ccgagttcgc ggccgccatg
     6781 cgcgcgaagt ggtatggcga gctggtcggt ggtctcgtgc tgcatccgta gccgcacgta
     6841 gcgcggtgat cgagcgaatc gacgacagtc gagcgtccgc gctcgctccg aacgctgcgc
     6901 gtgctcggat tacgcgcgct tcccgtggcg tcagccatgc acgcgccacc gatcatggcg
     6961 agacatcagg cgggtgttga tgaacgagat cgcgatctac cggcgggcaa gctgttcagc
     7021 atgtggactc ctatcctcgc gggagtcctg tccgtggtgc tctcggtcgt tgttctgatg
     7081 caggagtgat cggatcagag tagtggctcg gcgatcttga aggacttcgc tttgacccag
     7141 tcggccgccg agatgtcggc cgacgcgcga tcgctgtacc cgagcatcgc ctggagctcg
     7201 gtcgcgagct cggtcacggc ggtcccatcg cgcacggtca cgagcgcctc ggagttcgac
     7261 gccgcgctac gcggatcgag gttgtacgag ccgatgacgg cgagcttgcc gtcgatcagc
     7321 atcgtcttgg cgtgcatcgt ttcggtgccg cgatagtgat gtcggcgttc accaagcgcg
     7381 tcgattgtac ctgcgaggca aatcactacg tcggggaatc cgacgcattc tcggcgtacc
     7441 ggtctctcga ggttccacgt tgccgttcgg cagcttggcg cggtggaatc tcgaggttcc
     7501 aaaccagccg tttcaacgcg gtacgatggt tgctgtaggg gacgcatgac caagacacgc
     7561 ggattcctca tgctcgtggt tgctgcctgc ggcggaagca gcagtagcgg cgacgacacg
     7621 cctcccgatg ccagcgcgcg ttgtgtacct gtcgccggag ccgcggcgga gaccgtgatg
     7681 acgacgagcg ggctcgttca cggtgcggtt gcgaacgaga cgttcgcgta caagcgcgtc
     7741 ccattcgccg caccaccgat gggcgagctc cgctacgcgc caccgcaacc gcccgcgtgt
     7801 gcaagtggcg agatcgatgc gacggcgctc ggccccgtat gcccgcagct cgacaacgga
     7861 gccttcgtcg gcgacgagga ttgtctgcac ctcaacatct ggacgccatc ggcacctgca
     7921 ggtgcgccgc gcccggtgat ggtgtggatt cacggcggcg gcaactcgat cggttcggcg
     7981 gtgtatccga tctacgacgg gcgccggctc gccgaggtcg gcgatgcgat cgtcgtgacc
     8041 acgaactatc gactcggcca gctcgggttt ctcgcgctcg gcggtgacgg cgccggcgtc
     8101 tacggcatgc tcgatcagat cgccgcgctg cagtgggtgc gcgacaacat cgctgcgttc
     8161 ggtggcgatc catcgaacgt gacgatcttc ggcgagtcgg cgggcgcacg cgatgtctgc
     8221 tcgctcgtcg catcgccggc ggcgaagggc ctatttcacc gcgcgatcat ggaaagcgga
     8281 tcgtgcaagg cgctgccgac acgcacggcc gcagagcaaa ccgcggcaca ggtcgcgacg
     8341 accgtcggct gcgcagctgg cgacgtcgcg tgctttcgtg cgctgccggc ggaaacgatc
     8401 attcgtgcga acgcgtcgga catctcgggc ctgtcctccg gtgcctatca accgatgatc
     8461 gatggggtcg tgcagaccga tcaacccgac aacgtcatcg cggccggcgc gcattcgcac
     8521 gtgccgttcg tcgtcggtgc gaatgccgac gagactggca actcggcgcc cgcgatcatg
     8581 accgaagcgc agtacgacgc actcgtacac tcgcagctgc cgatggcgat cgccgacatg
     8641 gcgctgcagc aatatccggc gagcgcgtat ccaacaccgc gtgccgcgta cgttcgcctc
     8701 actaccgacg cacgcttcgt atgcccggcg cgggagatcg cgcgcgcagc cgatgccggt
     8761 cagagcgacg cggtctatcg ctacttcttc cagtaccgcg cctcgccgct gggcgccgta
     8821 cacggcctcg acgttccgta cgtgttcggc acgttcgatg gcatcgagac gccgaacggc
     8881 aacccgtaca cgccaaccgc aacggacctc gcgctctccg cgagcatcca gggctactgg
     8941 acgcgctttg ctcgtaccgg tgatccgggc ggcacacccg cgtggccgct gtacggcgcc
     9001 gcggatccga cgctcgtctt cgacgcgaca gtatcgacga cggatgggat ccgcaccgca
     9061 cactgcgact tctggcagcc gttctacgac gcgatctaga acacccacgc gagctgcgcc
     9121 tgcgcactcg acaccgccga gaattggccc tcggcgccga atcgcaagct ccgatacgcg
     9181 acttcgaatc cggcgaacgc gtgcccgacc agcgagtact ccgtatcgag ctcgaccatc
     9241 gacgaggtct cacgcgccag cacgccgtcg gcgccgactc ccacgtatgg cgtgatttga
     9301 tagcgcagcg tgcggccgac cgaaacatcg ccggtcagcg cgtgcatgtc catgtcgtcg
     9361 acgaacagcg tcttcgtgta cgcaccgcgg accgccactg tcaccggctt ctgctcgtcc
     9421 tgacggagca acgcgtgctt cacatcgacg ccgatccaac cgaagttcga catcggattc
     9481 atcgtgtagt acgcgccgat atcagtgttg ctaccgacgc cgacgcgcaa cttcagcttc
     9541 gggaactgct tgtcgctgcc gagccagtgg tccgccgttg gatgcgagaa cgtgtcgttc
     9601 cacgcatcgg ctcgttcgtc gatccgaaac gccatgtact cgatgccgaa cgacacgcct
     9661 ttcgtaccga ccacctgcgg cgcgcccatt tgcttgaacg cggacaccga gccgaactcg
     9721 cgcgcgaagc ggtgatatgc gctctgcgtc agctcgggcg caaagtgcac ctcgcactca
     9781 tcgaggctcg ggtcgacatg caaggtcggc gggtcatcag cgggagggtt ggcgtacgcc
     9841 gcggccggaa tcagaagtgc tgcaagggga acgatggtga tgcgcataag ggctctcctt
     9901 caccgaaacc atggctacgc ccctgcccgt ccgctttgac cgtccgtgcc aacgtcttcg
     9961 ccggctgcgc cacacgtgac gtcacccacg acacctggaa gggttagtgc cgagaacgcg
    10021 tacgaaacgc caccgcgaat cgagccggca aaaaattatg catagccggt ggccacacac
    10081 attgcggccc cgggacagcc tgtgtagagc agacgggatg accgatgacg gcgaggtcga
    10141 agacgactgg gatttctatc cctgccaggt cgacgacgcc gtggcttcga tcttcttgaa
    10201 cctgcgcttc gagcacgcga cgccaccgct agcagcgaac acgctgttcc gcatccgagt
    10261 gcagatgctc gagcccgacg agcacggcat gggcaccacg caggaagcga gcgtgctgaa
    10321 cgagatcgaa gacgcgatgg ctgagcgcgc cgcggagtcc ggcctcatct acgtcggacg
    10381 cattcgcagc ggcggcgtct gggaagtggc gttctacggc gcacccgagt gtaaggacgc
    10441 gctgcaagcg atgcgcgcgc tgtttccgga tcggcgaacg tatctggacg tcagacctga
    10501 tcccgagtgg ggtttctatc gcgagtttct gctgcctgat gaagagcgtc gccagtggat
    10561 gcaggaccgt cgcgttaccg atgcgcttgc cgagcgcggt gattcgctga ccgcgccgcg
    10621 acgcatcgat cactgggcgt acttcacgac tgccgagggc cgcgatcagt tcgtgggcga
    10681 cgtgaaggcg gccggcttcg cgctcgagcg cgcggcgcac gtcgaaggac acgagctacc
    10741 gttcgctgcg cagttctacc gcgacgacgc tgccgagctc gagcacattc acgacgtcgt
    10801 gatgttgcta ttcgagctcg cggagaagca cgacggtgac tacgacggtt gggagagtcc
    10861 ggtccaggaa gcgagctgag ctacttgtag accgtgtcga gccgccggtc ctcatcgccg
    10921 tacttgatcg agcagcgcca catcatctcg ttcgcctcga gcagccaacc gccgtcgtcg
    10981 aacgtgagcg tgccacgcat gatgacttcg ctctgatcgc ggatgcgcgc gggccactcg
    11041 ccgcggtaat gcacgcgcag ctccgcgctt ttcgcggtca gcacgaaccg gtggtcgtcg
    11101 ccggcgtgat cgatgctgcc ggcgcggatc caaccgtgta cgcggatgcc gtggccgaag
    11161 ctatcgctca ggcgcttcgt gacgagctcg tcgaccatca tgtacggcgc atctcgtcgc
    11221 acacgtgtcg cgaatgtgat cgtcagcgcg agcaccgcga ctgtcgcgag gagcctcgtc
    11281 gcgaatcggt catgggcgtg cgcgaggtcc atgtacgcgg caacaagccg cgccgcgagc
    11341 gcattcccgc gagcgcgcat cggcgcagcc gtagcactcg aaacgaggac cgcccccgcg
    11401 acccggatga ccgcgttcta cgtcgtctgc ctcggcgttg tcgccgacga cgtagaaccg
    11461 tcaggggtcg agggtgcggt gatagtaggc agtcagtgtc gtgatgaact gctgggccgc
    11521 ggcgcccatc aggatgtggt cttggccgtc ccagaagtgc gcttcggtgg ctcgacccgc
    11581 tgcatcgcga gctgctttga tgttgcgcga gcgctgcacc ggccacagcg tgtcggcgac
    11641 accgtggctg agatagagct cgcccgtgta gtcctcgacg cgaatgcgcg gaccggcctg
    11701 accgaactcg gtccgctcgc cgtagagctg cgtgccctgc cacgtccacg ccgcggccat
    11761 cttgtattgc ccgcttgcct ggtcgtattc gtagacggcg tcttcggtcg ccggatcgta
    11821 cgccgcgacg atcgtgtcgc tcggcgcatg tacaccgacc gtgtccatca tcgtcggtgc
    11881 gagactcgag agcaggacgg cctgctcggc accgcgtgac acaccgtaga gaccgacgtg
    11941 acggccaccg acggcgggac tggcgcgcag ccaggcgaga ccagcaagcg tgtcttcgag
    12001 cgggatgtga tagatgcgat tcgggcgacc ggaacagccg aaccagcaga acgtcaccac
    12061 cgcgaagccg gcttcggcga gtggccgcgc gtcgtcgtcg atgtacggag cgctgccgcc
    12121 ttcgcttccg tgcagcatga gaatgccggg cgcgggaatc gcgtcgggcc gcacgaacaa
    12181 gcgactgccg ggcagctccg ggatcgcagt gaccattacc tcgtgcgctg gcggtggacc
    12241 cgatccggcg tcgagcgttc cgccgctgct acctccacca ccgccaccac ctggatccga
    12301 tggatctggt gcgcctgcag ggcccgagca tgcgatgacg aagacgcaga taccgacgga
    12361 aaaccgcata gcgtggtgca acagcaaacg atatgccgcc gctctcgtca gctacgacaa
    12421 ctcgctgctc acttgtctgc tgcgcggtta gctgccgcga caatccgcga cacgatcacc
    12481 tgacgcacac ggtgttgcga cgcggaaaac gagtgctggt gagggtacgc gccgtcagtc
    12541 gatggacctc aggcgcgctg cagctcaggc gcgctgcagc gtttcccagc gcggccgccc
    12601 cgtcgcgtcg tcgatttcga ccgcgagctc gtaccgattg cctgtgcatt cgtcggccgc
    12661 gtcgaacagg agctgcaccg ggctgtggat ggcaagtgat gtttctccgc cgaccttcac
    12721 cggtaagcgc gggtccgccg catcgatcgc gagcgtcagg atccagttcg cgtcgaagtg
    12781 cgtgaggaac gcggtcatcg acttcttgac ctcgtgccgg gtccggcaat cgacgacggt
    12841 gccggcgaat ttcgcgatcc ccatcgcgtc gatcgtgcgc tagacgcgca acgaacacca
    12901 gcggcgtcgg tggtgactgt ccgcaatttg cactgttcgt cgtcgacaaa ctgtaacgtc
    12961 gtcgcgcacg cgacttcgtt tagcgttcga tcatgggcgg catcgtgcgc atcgcgatcg
    13021 tggtggccat ggtggcgtgt gcggccaacg atgcgcagac gccgcttcca ccgacggtgt
    13081 tgtcgtcgcc ggggccgctg atcctcggcc acgccgagct cgacgcgaag ggcgcgtgca
    13141 acagctgcca cgtcgacaac accgccgcac tcgacgcgaa caagtgcgcc gcatgtcatc
    13201 gcgagctgag tcagcggatg gcgaagaacc gcgggttcca cgcgtcggcg accgtgcacg
    13261 gcaagccatg cgaaacgtgc cacgtggatc atcgggggcg cggctacgac ccgatgggct
    13321 ggaagagcct caagggcggc cgcgacggtt tcgatcacgc tctcactggc tggccactcg
    13381 acggtgcgca cgtcacggca gcatgccgcg actgccacag cgagcgtgat accgccggcc
    13441 tcgagacgta tctcggcacc gacacgctgt gtggcacgtg ccacggcagc ccgcacaact
    13501 tcacgaagcc cgcgctcgag gcgtgcgatc gctgtcactc ggaggcagcc tggaaaccag
    13561 cgctgccacg gccgcagttc aaccacgaca atcggctgga cgcgcgcatg ccactgctcg
    13621 gcgcgcacgc gagcgtcgcg tgtcgtgaat gccatgcgaa cggattgttc aatctcggtg
    13681 tcgcgcagcc cgatcgctgc gagacctgtc atgactcgcc tcacgccggt gggctgtacg
    13741 cgaagaagga ctgcatgtgg tgtcactcgc cgacgttcaa gtcgttcgac aagacgttct
    13801 tcgatcacac agagcacacg cgcttcgaca tcggcggcca caaggatctg ccatgcgcgc
    13861 gctgtcatcc cgcatcgctc ggtgcccaga aaccggcgat ggcgtgcgag acatgtcacg
    13921 ctgctcgatc gccgcacgcc aagcgcttcg cgcaatacgg ctcgccgccc gcctgcgcga
    13981 agtgtcactc gacgtcattc ggcacgccgc cgcgcgcgcc gtggcgaccg aaagggttcg
    14041 atcacacgaa gaacaccgta ttcccactcg tcggcaagca cgcggagctt gcctgccgcg
    14101 cctgtcatac cggctcgggc ccgacgagtt tcgagaagct cgacatcaag aagggctgca
    14161 tgggttgtca cacgcacgag gccgttcacg acaagaagtt caaaaacgag cagtgcctgc
    14221 tctgccacgt atcagccggc cactagccga taacaatcgc catcgtgatc gcggcgacca
    14281 tcgcagcggc gagaggagca atgacgacaa cgctgatgag gctgatgagc ttgttcatat
    14341 ccggacacac gtaggtccgc gagaaaccct gcaacgagct cgtaagtgct tgcgaacaga
    14401 actgtttcaa cggcaatgct tgcctttgaa aaccgaccgt gggatgtccg gcaacggtct
    14461 gccgatggat cgtcgagaca acagagtatc ggcgctacgc ctggcgacga cggccgcggt
    14521 tgtccttgtc gcctgccacc tcggcgcacg cttcggcacc tggctgcgat tcccagagat
    14581 cggatctgcg ttgctgtttc cgccctacgc gatcgtgacg gcggcgttgt gtcggttgcc
    14641 gccacgacac tggtggatcg tgctgctcgc tgcaggtctc ggcgacatcg gaccgcacct
    14701 gcgcggcggc gccagcgtca cattcgcgct gtgcgcggag ctcctgaacc attcgcgcgc
    14761 tgtgctcgcc gcgatcggta ttcggcggtt cgccggcaag atcgagaccc tgcgcgagat
    14821 ggtcgcgtac atgatcatcg cggtcgcggt cgcgccagcg ctcgccgcga tcggcggcgc
    14881 ggtcctggtg accacgctcg gcacgtccga cgacttcctg ctcgcgtggc agcaatggtg
    14941 gctgtcgaat gcgatcaccg cggtgacgct gttgccggtc ttgctgctgg attttcgaaa
    15001 gctcgctgcg ttctcgccgc ggcgcctcgt cgagaccacg ctgtatgtcg tcgtgctcgt
    15061 tgtcggcacc gggtggacgc tcgagtacgt ccacccgaca cacatgtatt gggcgctgcc
    15121 gtttctgctg ctggcagccg ctcgattcgg tgcgcacgtc accagcgccg tgctgctcgc
    15181 ggtgacgatg ctgtcgatct ggggtgcggt cggccgcagt gacgggttcg tcacttcatc
    15241 cgagagcctg ctcgagctcc aggtgttcct gatcgatgtg gcgattccac tgctgctgct
    15301 cgccgtgttg atcgatcagc agcgacgaac cgcatccgag ctcacgctca gtcgtcggca
    15361 aatccaatcg atcgtcgaag accagacgga gatgatctgc cgcttccgtc ctgacggcac
    15421 gtgtacgttc gcgaaccgcg cgtactgcga ggcgttcgcg gcgtccgatc cggttggtcg
    15481 gtcggtgtgg cagctcgcgc ccacgggcgt gcaccatacg gcgttggagc tgcagcaatt
    15541 gacgcgggca tctccgctcg cgactcacga agtcagcgtg cccacgacgg accgcgcgct
    15601 acgatggcag caatggcagg agcgcgggct gttcgatgaa cgcggtgcga tcgtcgagta
    15661 ccaagcagtc ggtcgcgaca tcacggatcg caagcgtgcg gaggacgagc acgccgagct
    15721 cgaagcccaa cgatccgtgg aggcggcgct gcgcgaagcc gatcggcgga aggacgaatt
    15781 tcttgcgatg ctcggtcacg agctccgcaa tccactcgcc ccactgggca tcgcggtgaa
    15841 ggtgctcgtc gaagcaccac cgggaagcag cgaggccgca tgggcgcgcg gctcgatcga
    15901 gcggcagctc ggcaagatga cgcgcctgat cgatgacctg ctcgacctat cgcgcgtcac
    15961 gctcggcaag atccagctcc agctcgacgc ggtgaacctc gcaaccgtga tcgaaagcgc
    16021 ggtcgaagaa acgcgtccac tcatcaactc gctcggccac caactgatcg tcacactgcc
    16081 ggacgcgcca gtgtggctcc gcgccgatgc cgtgcgcctc acgcaggtcg tcgcgaacct
    16141 cttgcacaac gccgccaagt acaccgaacg cggcggtcgc atcgaggtca cggtgacgcg
    16201 cgacgacgcg ggcgtgtgca tcgcggtacg cgacaatggc atcggtctcg acccggcctg
    16261 cctggagacg atcttcgggc tgttctcgca attgcccgac gcacgcgagc gtgcacaagg
    16321 cggactcggt atcggtctca cgttagtgag gcgcctcgtc gagctgcatg gcggaagcgt
    16381 cgtcgcgcgc agcgaaggtc gcaactgcgg cagcgagatg gtggtgcatc ttccaatcgc
    16441 cgcgcaggac gcgctcgcgc cgagccgccc cgttacgccc caggtaacac cgcgagcgtc
    16501 actgcggatc ctcgcggtcg acgacaacgt cgacatcgcg aaaggtctgg cggcggtact
    16561 cggcctgtgg gggcacacgg tgcagacggc gcacgatggc gaagccgcgc ttgcgatcgc
    16621 gacggcgttc gcgccggatg tggtgcttct cgatctcggt cttcccagca tcaacggcgt
    16681 cgaggtcgcg caccggctcc aaacgtccga gtacgcgccg acgctgctgg tctcgatgtc
    16741 gggcttcggc aacgactacg tgcgccagcg cgccgccgag aacgggttcg actaccacat
    16801 cgtcaagccg atcgacctcg actggcttcg tacgctgctg gacaagtgcg cgcacgacaa
    16861 gccgcgcgcc cccaccgcct agtggaccat gaagtcgcgg atcagcttcg tcggctggcc
    16921 gggcaggatc gcgacgtcga acgtcttgtc gatcttgcga gcggtgttga acaacgtgac
    16981 cttgtgcttg ccgggcttca gcttgatcgc acgctgcggt gtcatcagca tggtggcgac
    17041 gccgtcgacc gcgatctcgc acggcggctt gctcgacacc atcaagatgc cggtaccacc
    17101 cttcggtgtc tccgccgcaa cgcgcgtcgg gcgcggggct gctagcgatg gccgctgctc
    17161 cggtcgcttc gcacgctcgg tcggcgccgg agtcgacgcc actgcctcgg ttcggttcac
    17221 tcgcgtcggc ggcgccggtg tcgatgtctc ggcgcgctct tcgcgctctt cgtgggtcgc
    17281 cttcgcccgc gcaggtgcgg gttcgtcgtt cgaaacgagc acgacatgaa cctcgtcggc
    17341 cttcggatcg atgcgagcaa gccgcgtccg atagcccggc cgcgtaagca cgatgtcgta
    17401 ggttagcgcg ggatcgatgt tcacgttgag cggcgtcaca ccgacgaccg atgtagcgcc
    17461 attcgtgatc aacgtgaccg tcgcgccttc cggctccgtg gtgaacggaa gctcgcgcgt
    17521 ggtcggcgac gcaggcggcg gctcggcggc cgcaacttgt ggctcggctg ccggtgcgtt
    17581 cgcaaccggc gcgggctcag gcgtggtcgc agcaggcgcg ggctcgggaa tcaccgcggc
    17641 aggcccagcc tgttccggcg tcgcaacggg cgcgacggcc ggtgctgcgg gcgcgacggc
    17701 cggtgctgcg ggcgtgacga cgggcgccgg cgtcggcgcc aacgtttgct cctgcgcgat
    17761 tgccgcgtgg tgcgtacgcc gcgccggtgc gtcgtgatca ccgcgaagca ggatcacggt
    17821 catcgctgac aggccgccga cgagcaccgc cgcgatgccg agcttgaaga ccggaatcgg
    17881 cgcgaccttg cgatggaggt tcgacgtgct gtcccacagc agttcgtccg cgtccgcctc
    17941 gcgactgtcg gtcgaggcca tgctgtcgaa ccagatctcg tccgtgtgct cgacgtgagg
    18001 acgcggcagc ggcgtcggat cctcgtgacg tgcgggcgcc ttcgcctcga cgacgacggg
    18061 gaatgcagac accgccggga gcactgacga tgtcgcaggt ggcgtcggtg ccggcgccac
    18121 gacgaacgac gaggtcgatg accggatcgg tggcggcaac gtcccacgcc gcgcgtcgcg
    18181 tgcaagcggc ggcggcgtcg gcagcgacgt gcgcgctgcg ctcaacatga aggtcggtag
    18241 tggcggcgca accgcgggag cttcttcctc ggccggcgac tctgcaccgt cggtcgccgc
    18301 atcgctcgca agctcggtcg tgcttgccgc cgccgcgaga ctcgcgggct cggcattgtt
    18361 accaagcgac cagtcgctcg caggctcgac cgtgccgctg atcgaccagt cgtcggcgag
    18421 ggccaacgat ggcacatcga tcaccgcggc atcgcgcgta gccgggattg gagccgtgcc
    18481 acgcggcgcg atatcgagcg gcgccgggtc gcgctccagc gcttggtcgc tcgggggcgc
    18541 ttccgtcggc gcgaacgacg tcagatcgac atcgactgct tcttcggtct tcggcaccag
    18601 cgtccgaccg ctctgcgtca acgcgcgctg cggaatcggg ggtggcaccg acgccgcgcg
    18661 catcgggcgc gacgtgcgag cggcatcggg aatcggtggt ggtaccgatg cagcgcgcat
    18721 cgagcgcgac gtgcgagcgg ccgcaggaat cggcggtggg gtcgcgcgcg gtttcgactc
    18781 gccgtcagcg ggagcatcga ggccaagctc gtcccgatcg aacgacggtg gcgctcgttc
    18841 ggtgagatcg agttgttcga gatcgcgatt tggttcggac atcacggact ccgttcgcca
    18901 aaacacctgg cgtaagaaga tggccccctc ggccgcgcag gaacgttagt cgccgcagtg
    18961 cgtgatggat cgtcgttcac ttgacagcga gtcaacggaa ctgcggcgac gggttcgcac
    19021 aggcgaagtg gcgcggcctc actgcccgcg caactgtgca gtaggatgcg cgagccgtg
//
LOCUS       DASUFX010000570        10578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_925739,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000570 DASUFX010000000
VERSION     DASUFX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925739"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..807
                     /locus_tag="VFV99_22855"
     CDS             427..807
                     /locus_tag="VFV99_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062236.1"
                     /translation="MEKRPGGKAVRDTRTGVTIDFLITGEYPGDGKPKPVQFPDPSTN
                     VVRSTWHGKHVAVLSVPRMLELKLASGISAPHRMSDLADVLKWIRHTNLPRELADQLD
                     PYVRPKYLELWDLAQTPDPIAEDR"
     gene            complement(804..1097)
                     /locus_tag="VFV99_22860"
     CDS             complement(804..1097)
                     /locus_tag="VFV99_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062237.1"
                     /translation="MQVAAEATLMALEYKFVELSIVTDETIEEAVNAWVARGWLFDGI
                     RFVTTEASRRPQMAFISFTREVTRASVDAHDPADATLPRLKGPRLVKDEDETD"
     gene            complement(1070..2020)
                     /gene="hemB"
                     /locus_tag="VFV99_22865"
     CDS             complement(1070..2020)
                     /gene="hemB"
                     /locus_tag="VFV99_22865"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5062238.1"
                     /translation="MRRTENLRRMIRETRLAPEDFIYPLFVVPGSGVAKPISSLPGQF
                     NFSIDKAVDAAAAAADLGIPAVILFGIPEKKDAVGSEAWKETGIIQKAIRAIKKARPE
                     LIVMADACFCEYTDHGHCGVLHGGELDNDASLENLARTVVSYAKAGVDVVAPSGMLDG
                     FVGACRESLDEDGHDQVAILAYSAKYASGYYGPFREAVDSAPQEGDRRGHQMDPANVA
                     EAIREVSLDVAEGADIVMVKPALAYLDVIRAVSDEVNMPVCAYNVSGEYAMIEAAAER
                     GWIDKERVVLETLLGIRRAGADMIISYHAPYACKLLQKQR"
     gene            complement(2051..2827)
                     /locus_tag="VFV99_22870"
     CDS             complement(2051..2827)
                     /locus_tag="VFV99_22870"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5062239.1"
                     /translation="MAVAVLTRDPGDVTAYAAALAPLGLDVVAMPVTKTTAPPDPDAL
                     TRALDTGDYAAIVVASARAAYELSRAVAALANVRTTMPDLPDVWAVGPATKRALDIAK
                     LPAHVPDGVRDGAELAGKLVATKKLAGKRVLVPRAEDGRVETLAILRAAGAEVVDIVA
                     YRTVALAPDDPALTEGADLLVRGGAAICALFAPSQVTALGAALAARDHELPSVVTQFC
                     AIGETTAAALRAAGIRDVAVAVAPTPEGMARAVSTVYPGR"
     gene            2882..3355
                     /locus_tag="VFV99_22875"
     CDS             2882..3355
                     /locus_tag="VFV99_22875"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX5062240.1"
                     /translation="MIGSAPYKAPSMPAPSGTRRDTELDRVRSLAKVLDQYFLDPVLG
                     LIIPGGGDVLGAMLGTYVVMIAARRKVSPVIIARMLMNLAADTAFGIIPLFGDIFDFK
                     FKANLRNVALLEERSRHGGRATVRDWLILISAICAFIGVLALVIWGVVALVRRIF"
     gene            3365..3859
                     /locus_tag="VFV99_22880"
     CDS             3365..3859
                     /locus_tag="VFV99_22880"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX5062241.1"
                     /translation="MRFALLLTVTIACSTGTKREDSAAPMPPPPPTKPEAPYTGPKVF
                     VTSTKGDVEVKVEVVATEAKIQKGLMFRQHLPLDAGMLFLMGQEREWPFWMRNTLIPL
                     DMLFITSDMKVAGIVENAEPLTESLRTVGKPSLYVLEVNGGWSKSHDVIAGATVRFEN
                     VPKQ"
     gene            complement(3860..6613)
                     /locus_tag="VFV99_22885"
     CDS             complement(3860..6613)
                     /locus_tag="VFV99_22885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5062242.1"
                     /translation="MRKPVLALIGVAVVGACVWWFFLRSPSRPSETHGTAKTEGKVTQ
                     PKPAQTQDRGSEREPLSFLVDDDPKGTLRLEGQVVDETDKPVAGATVVLSSNPPRTAT
                     SEDDGGFAFDALVARPYTLTARAAQGVAGPVTAKLTEKSDPVVLQLRPGGKVTVTVQG
                     TDGKPVHNATVELRSTDVLRQTTKAGTTTFSSVVPGGYQLAAWAEGRAHSLQWLQVGP
                     GETAAKITLVAGAKVSGRVIDVGGKPVAGARVTYHGASDWNQQADERLDAVETDAKGA
                     FTFAALPAGSFRFAAGHPEFARGQSALVTLDGKNEMRDITITVDAGAVVRGIVTDQAK
                     QPISGARVRIGIASRRGMVFEPPRQAYSDGKGSFEIKGLGRRELQAVAMHETGASQTV
                     DVDTTRGDVSDVRLVLDVTGTIAGIVVDPSGTPLEGIQVSAGPNFRTQGTPDMSAFRL
                     RGFPQDLTDASGHFTLVGLAKGSYQVYASRASSASRGRFMANPDGGTIAETGQKDLRL
                     VLQPEGGVKGKVQLADGGTPALFTVQVGFAQQSFSGSDEFVLDALQPRKYELSVRGPT
                     FQTKVIEVDVQPSKTTDAGTITVQKGRTLAGTVIADGQPVAGATVYAGRQLFGNGTSN
                     AANFGPMGQGAKQDTTDANGKFSIAGFNAGDISIVADHPDVGRSKALRVPTNLPGQTE
                     LVLELQKAGSIKGVLRVAGKPAEGVLVSCQSTTTPGAIYGVAAGPDGGYRYDRLAPDT
                     YKISATVGMPMMGMKFYSKEVVVPPGKEVTVDLAVEPGAITVNVQPVAKTGKVGVGSA
                     ILISGVVVARTANDLQMRTASAGQSAMQWVIIRNGEPAKFAEVTPGTYSACVVPFPAE
                     VQGMAAMGYVDRHGDALPAFCQRVAVAPAPETQTATVQVEIPPYEPDDPGAGSGSGKP
                     PRP"
     gene            6720..7703
                     /locus_tag="VFV99_22890"
     CDS             6720..7703
                     /locus_tag="VFV99_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062243.1"
                     /translation="MAFRPFLLAAAAMLPMSLVACGGDDGQPVPEGTHYHFVANQVFV
                     PTSNTQARDFGLDLNGDNTVDNQLGMVLSTLAGMGFDIQATIDTAVAEGSIILLVDFQ
                     TKDFTNTTAAGVQVFLGDNEMPAACNSGESYDPVAKSGCKHHLDGSGMFSIAAGSPEN
                     AALGGKIVNGTFTGGPGDLSLQIALGGTDAIKLDLIGARAKASMISENGIGMGTSGGV
                     VFGGAVTKDDIDNKVIPAIQTQLVPIIMRDCTMPTMPPGCGCATDSTGKTILGLFDTN
                     PKDCAVSVEEIKTNSLIVSLLSPDVTINGKMALSLGIKATAVKGTYTAPNQ"
     gene            7895..8380
                     /locus_tag="VFV99_22895"
     CDS             7895..8380
                     /locus_tag="VFV99_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062244.1"
                     /translation="MKRFALVALVAGCAGATPVVLTGRAETASFPGRVTMQLAAYSGP
                     SSVCDPSDSANLCSAATVIARVGHILDGVRRVPCSDAARALDRYADLHAHEIATLLHL
                     HELEPAASLARWEDRQRGTAELALVAGFDLVTRCEHDDQLEHAVRRVGLGSLFARSTS
                     R"
     gene            complement(8367..>10578)
                     /locus_tag="VFV99_22900"
     CDS             complement(8367..>10578)
                     /locus_tag="VFV99_22900"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5062245.1"
                     /translation="NTIGKTVTDKDGHFTLTGIPLGDYTIEASSPGLVEDHQHLQITS
                     SQVLTVELVLVDTEEVIRIEEDWALPPPPKATGSVVAVGRQTLAELPGADDRPITDVM
                     AKAPGFVEDSLGEVYVRGQHGNIQYQVDGIPVPDSVGSMFAASIPVRLVQQLEIMTGG
                     IPAEYGYRLGAIVNLVTRNAAEHPEGNLSLRYGSFDTVEPGLVYSAKLSDRTGMVIGG
                     SATYSQRALDAPSIDPILHDTGLSARVFTRFDYQPCESNRYELFAMYAHNRFEVPIDP
                     AARPFEPTRPRPVDKFGNEAPAFIPHDTDAREHEHEAFAAFSWIHKLQRGQLMLAPLY
                     KLSRGTLVGDAEHALGDGADPGSTASDVKRTAHHAGGIAAYSWQNGRHLVKSGLQTDF
                     LYGSSHFTLYERDDVNGGIASSTSGHDRTEALLSGAYVQDHVTLGDAALDAGIRIDQL
                     HVMLQGGGTDDSFGVSPRLGLSYSFAKDVVGHVFSGVMWLPPSPLDAASAARALGVVP
                     ADQPVTYDLKPETDLYSEVGILARLSKLLRGGLTAWGRYSYNQLDDTAIGSTSLDSNY
                     NFKRGRAAGLEGSLELRVGPWLSGFANASLGFAQGRGIASATFLFSPDDLADNTWQSL
                     DHAQTWTGNAGATVRDGRFTVTGMFTYGSGLRTGPANDQHVPGHLVADLSSQYTFVAR
                     EYPIRLGIDIENLFDRRYAFRIGNGFVGSSYGAPRSVFVSLSIPLATEPHINEK"
BASE COUNT         1705 a         3468 c         3558 g         1847 t
ORIGIN      
        1 ctgactcgaa tcgcgctcca cgagctgctc gacgcgctcg caccgtacgt cacgcgccag
       61 gatcgcgaag cacgaagcgt gttgctcgac ggcgagccgc tcgacgcggc gctgcagcgg
      121 atgttctgtg cttgatcggc gagggtgggg tacgcttagc gtgccgtgtc ctcacctctc
      181 gatcgagatc gcgacgcacg cttcatcgaa agcatcgaag aaagcggccg gttctttatg
      241 ggtgaggaca aagtacacga cgcgctgttt gcgattacac gggcgctcga agatgcgcag
      301 attccgtatg cgattgtcgg tgggctcgcg ctcaacatgc acggataccg gcgagtgacc
      361 gttgatgtcg atatcctcct aacgcgcgct tcgttggatg cattcaaggc gcgctatctt
      421 ggttatttgg agaagcgccc aggcggcaaa gccgtacgcg atacgcgaac cggcgtaacg
      481 atcgactttc tcattaccgg ggaatacccc ggcgacggga aaccgaagcc cgtgcagttc
      541 cccgatcctt cgacgaacgt cgtccgcagc acgtggcacg gaaaacacgt tgcggtcctc
      601 tcggtaccgc ggatgctcga gctcaagctc gcgtcgggca tcagcgcacc gcaccgaatg
      661 tcggacctcg ccgacgtgct gaagtggatc cggcacacga acttgccgcg cgagctcgcg
      721 gatcaactcg atccgtacgt acgaccgaag tacctcgagc tctgggacct ggcgcagaca
      781 ccggatccga tcgcggaaga tcgctagtcg gtctcgtctt cgtctttgac gagccgcggg
      841 cccttgaggc gcggcaacgt cgcatcggcg ggatcatgcg cgtccacact cgcgcgagtg
      901 acttctcgcg tgaacgagat gaacgccatc tgcggccggc gcgacgcttc ggtcgtgacg
      961 aaccgaatgc cgtcgaacag ccagccgcgt gcgacccacg cgttgaccgc ttcctcgatg
     1021 gtctcgtcgg tgacgatgga cagctcgacg aacttgtact cgagcgccat cagcgttgct
     1081 tctgcagcaa cttgcacgcg tacggtgcgt ggtagctgat gatcatgtcg gcgccggcgc
     1141 ggcgaatgcc gagcagcgtc tcgagcacga cgcgctcctt gtcgatccag ccgcgctctg
     1201 cagctgcttc gatcatcgcg tactcgccgc tgacgttgta cgcgcacacc ggcatgttca
     1261 cctcgtcgct gaccgcgcgg atcacgtcga ggtatgcgag cgccggcttc accatcacga
     1321 tgtccgcgcc ttcggcgaca tcgagcgaga cctcgcggat cgcctcggcg acgttcgcgg
     1381 gatccatctg atgcccgcgg cgatcgccct cttgcggcgc gctgtcgacg gcctcgcgga
     1441 acggaccgta gtaccccgac gcgtacttcg cggagtaggc gaggatcgcg acttggtcgt
     1501 ggccgtcctc gtcgaggctc tcgcggcacg cgccgacgaa gccatcgagc atgccgctcg
     1561 gtgcgaccac atcgacgccc gctttcgcgt agctgacgac cgtgcgcgcg aggttctcga
     1621 gcgaggcgtc gttatcgagc tcgccgccgt ggagcacgcc gcagtggccg tggtcggtgt
     1681 attcgcagaa gcaggcgtcg gccatcacga tcagctcggg gcgcgccttt ttgatcgcgc
     1741 ggatcgcttt ctggatgatg ccggtctctt tccacgcctc ggaaccgacc gcgtccttct
     1801 tctcgggaat gccgaacagg atcacggcgg ggatgccgag gtccgcagcg gccgcagccg
     1861 catcgacggc cttgtcgatc gagaagttga actggccggg cagcgagctg atcggcttcg
     1921 ccacacccga gccaggcacg acgaacagcg ggtaaatgaa gtcctcgggg gcgagccgcg
     1981 tctcgcggat catccgccgg aggttctcgg tgcgcctcat ccggcgaggc cggtaatcgg
     2041 ggtaattcat tcagcgacct ggatacaccg tggagaccgc gcgcgccatg ccctcgggtg
     2101 tcggggcaac cgcgaccgcc acgtcacgta ttccggcggc acgtaacgcg gctgcggtcg
     2161 tctcgccaat cgcgcagaac tgcgtgacta ccgaaggcag ctcgtgatcg cgggcggcga
     2221 gcgcggcgcc cagtgccgtc acctgtgagg gtgcgaagag cgcgcagatc gcagcgcctc
     2281 cgcgcacgag caggtcggca ccctcggtca gcgccggatc atcgggcgcg agcgcgacgg
     2341 tgcggtaggc gacgatgtcg acgacctcgg cgcctgccgc gcgcaggatc gcgagcgtct
     2401 cgacgcggcc gtcctcggcg cgcggaacca gcacgcgctt gccggcgagc ttcttcgtcg
     2461 cgacgagctt gccggcgagc tcggcaccgt cgcgtacacc gtcgggcacg tgcgcgggca
     2521 gcttcgcgat atcgagcgcg cgcttcgtcg ccggcccgac cgcccacacg tcgggcagat
     2581 caggcatcgt cgtgcggacg ttcgcgagtg cagcgaccgc gcgcgacagc tcgtaggcag
     2641 cacgcgcgct cgcgacgacg atcgcggcat agtcgccggt gtcgagtgcg cgcgtcagcg
     2701 catcgggatc cggcggcgcg gtggtcttcg tcaccggcat cgcgaccacg tcgagcccca
     2761 gcggcgcgag cgcggcggca tacgccgtca catcgcccgg gtcgcgcgtt agcactgcta
     2821 ccgccatacg tcgaggttag tccacctggc acgcgatgtg ctgttgctaa ccggcatggc
     2881 gatgatcgga tctgcaccgt acaaggcgcc gtcgatgcct gcaccgtcag ggactcggcg
     2941 agataccgag ctcgatcgcg tgcgcagcct cgcgaaggtg ctcgatcagt actttctcga
     3001 tccggtgctc ggcttgatca ttccgggcgg cggcgacgtg ctcggcgcga tgctcggcac
     3061 ctacgtcgtg atgatcgcgg cacgccgcaa ggtgtcgccg gtcatcatcg cgcgcatgct
     3121 gatgaacctc gcggcggaca ccgcgttcgg catcattccg cttttcggcg acatcttcga
     3181 cttcaagttc aaggcgaacc tgcgcaacgt cgcgctgctg gaggagcgtt cgcgccacgg
     3241 tggccgcgcg accgtacgcg actggctgat cctgatcagt gcaatctgcg cgttcatcgg
     3301 cgtgctggca ctcgtgatct ggggagtcgt tgcactcgtg cgccgtatct tctaggttct
     3361 ccgggtgcgc ttcgcgctgc ttctgaccgt caccatcgcg tgctcgactg gcaccaagcg
     3421 cgaggacagc gctgctccga tgccaccgcc tcctcccacg aaaccagaag ctccgtacac
     3481 cggaccgaag gtcttcgtca cgtcgacgaa gggcgacgtc gaggtcaagg tcgaggtcgt
     3541 cgcgaccgaa gcaaagatcc agaaggggct gatgttccgc cagcacctgc cgctcgacgc
     3601 cggcatgctg ttcttgatgg ggcaggagcg cgagtggccg ttctggatgc gcaacacgct
     3661 catcccgctc gacatgttgt tcatcacgag cgacatgaag gtcgccggca tcgtcgagaa
     3721 cgccgagccg ctcaccgaat cgctgcgcac cgttggcaag ccatcgctct acgtgctcga
     3781 ggtcaacggc ggctggagca agtcgcacga cgtgatcgca ggcgcgaccg ttcgcttcga
     3841 gaacgtgccg aagcagtaat tacggcctcg gtggtttgcc cgagccactg cccgcgcccg
     3901 gatcatcggg ctcgtacggt ggaatctcga cctgcacggt cgcggtctgc gtctccggcg
     3961 ccggcgcgac ggccacgcgc tggcagaacg cgggcaatgc atcgccgtgg cgatcgacat
     4021 agcccatcgc tgccatgcct tggacttcgg cggggaacgg cacgacgcac gcactgtagg
     4081 tgcctggggt cacctcagcg aacttcgccg gctcgccgtt gcgaatgatc acccactgca
     4141 tcgcgctctg gcctgcactc gcggtgcgca tctgcagatc gttcgcggtg cgcgcgacga
     4201 cgaccccgct gatcaagatc gcgctgccga cgccgacctt gccggtcttc gcgacgggct
     4261 gcacgttcac cgtgatcgcg ccgggctcga ccgcgagatc gaccgtcacc tccttgccgg
     4321 gcggcacgac gacctctttc gaatagaact tcatgcccat cattggcatg ccgaccgtcg
     4381 ccgagatctt gtacgtgtcg ggcgcgagtc gatcgtagcg ataaccgcca tcggggccgg
     4441 cggcgacgcc gtagatcgcg cccggtgtcg tcgtcgattg gcagctgacg agcacgccct
     4501 cggctggctt gccggcgacg cgcagcacac ctttgatcga gccggctttc tgcagctcga
     4561 gcacgagctc ggtctggcct ggcaggttcg tcggcacgcg cagcgctttg ctgcgaccga
     4621 catcgggatg atcggcgacg atcgagatgt cgcccgcgtt gaagccggcg atcgagaact
     4681 tgccgttcgc atcggtggtg tcctgcttcg cgccttggcc catcggaccg aagttcgccg
     4741 cgttgctcgt gccgttgccg aacagctgac ggcccgcata gacggtcgcg ccggcgacgg
     4801 gttggccgtc ggcgatcacg gtgccggcga gcgtgcggcc cttctgcacc gtgatcgtgc
     4861 cggcgtcggt tgtcttgctt ggctggacat cgacctcgat caccttggtc tggaacgtcg
     4921 gaccgcgcac gctgagctcg tacttgcgtg gctggagcgc gtcgagcacg aactcgtcac
     4981 tgcccgagaa cgattgctgc gcgaagccga cctgcaccgt gaacagtgcg ggcgtaccgc
     5041 cgtcggccag ctgcaccttg cctttgacac cgccttcggg ctgcagcacg agcctgagat
     5101 ccttctgacc ggtctcggcg atcgtgccgc cgtcgggatt ggccatgaag cggccgcgac
     5161 tcgcgctcga cgcgcgtgat gcgtagacct ggtagctgcc cttcgcgagg ccgacgagcg
     5221 tgaagtgacc gctcgcatcg gtgagatcct gcggaaagcc gcgcagccgg aacgcagaca
     5281 tgtctggcgt gccttgcgtg cggaagttcg ggccggcaga tacctggatg ccttcgagcg
     5341 gtgtgcccga tggatcgacg acgatgccgg cgatcgtgcc ggtgacgtcg agcacgagcc
     5401 gcacgtcgct cacgtcgccg cgcgtggtgt cgacatcgac ggtctgtgac gcgccggtct
     5461 cgtgcatcgc gaccgcttgg agctcgcgcc gaccgagtcc tttgatctcg aagctgcctt
     5521 tgccgtcgct gtacgcctgg cgcggcggtt cgaacaccat gccgcgtcgc gatgcgatac
     5581 cgatacgcac gcgcgcgccg ctgatcggtt gcttcgcctg atcggtcacg atgccgcgca
     5641 cgaccgcgcc cgcatcgacg gtgatcgtga tgtcgcgcat ttcgttcttg ccgtcgagcg
     5701 tgacgagcgc ggactggccg cgtgcgaact cgggatgccc ggccgcgaac cggaacgatc
     5761 cggcgggcag cgcggcgaac gtgaacgcgc ccttcgcgtc ggtttccacg gcgtcgaggc
     5821 gctcgtcggc ttgctggttc caatccgatg caccgtgata ggtgacgcgt gcgcccgcga
     5881 cgggtttgcc acctacatcg atcacgcgcc ccgacacctt cgcgccggcc acgagcgtga
     5941 tcttcgcggc ggtctcgcca ggaccgactt gcagccactg cagggagtgc gcgcggccct
     6001 cggcccaggc ggcaagctga taaccgccag gaacaacgga cgagaacgtc gtggtgcccg
     6061 ccttcgtggt ctggcgcagc acatcggtag aacgcagctc gacggtcgcg ttgtggacgg
     6121 gcttaccgtc ggtaccctgc acggtcacgg tgacctttcc gccgggccgc agctgcagca
     6181 cgacgggatc gctcttctcg gtcagcttcg cggtgacggg gcctgcgaca ccctgcgcgg
     6241 cccgcgcggt caaggtgtag ggccgcgcga ccaatgcatc gaacgcaaac ccgccatcgt
     6301 cctcactcgt cgccgtgcgc ggcggattcg aggacagcac gaccgtcgcg cctgcgaccg
     6361 gcttgtccgt ttcatcgacg acctggcctt cgagccgcag agtccccttc ggatcgtcat
     6421 cgaccaggaa cgaaagtggc tcgcgctcag acccgcgatc ttgcgtctgc gcgggcttgg
     6481 gttgtgtgac cttgccttca gtttttgcag tcccgtgggt ctcgctcggt cgtgacggac
     6541 tccgcagaaa gaaccaccag acgcacgcac cgaccaccgc gacaccgatc agtgcaagta
     6601 ccggcttacg catcggcgcg gagtatggct cggatcacgg gtgctcgggc ctgtttacgc
     6661 ttattgtgca gcgtcggcgt ttcgggataa aacgccttac cacttacgag gggattcaaa
     6721 tggccttccg tccattcttg cttgctgctg ccgctatgtt gccgatgtcg ctcgttgcct
     6781 gcggaggcga tgacggacag cccgttccag agggcacgca ctaccacttc gtcgccaacc
     6841 aggtgttcgt cccgacgtcg aacacccagg cccgtgactt cggtctcgac ctgaacggcg
     6901 acaacacggt ggataaccag ctcggcatgg tgctcagcac gctcgcgggc atgggcttcg
     6961 acatccaggc gacgatcgac acggccgtcg ccgagggcag catcattctg ctcgtcgact
     7021 tccagaccaa ggacttcacg aacacgaccg ccgcgggcgt ccaggtgttc ctcggcgaca
     7081 acgagatgcc ggccgcctgc aactcgggtg agtcgtatga cccggtggcg aagtcaggct
     7141 gcaagcatca cctcgatggc agcggcatgt tctcgatcgc ggcgggctcg ccggagaacg
     7201 cggcgctcgg cggtaagatc gtcaacggta cgttcaccgg tggccccggc gacctgtcgc
     7261 tgcagatcgc gctcggtggc accgatgcga tcaagctcga cctgatcggt gcgcgcgcga
     7321 aggcgtcgat gatcagcgag aacggcatcg gcatgggcac ctcgggtggc gtggtcttcg
     7381 gcggcgcggt cacgaaggat gacatcgaca acaaggtcat cccggcgatt cagacccagc
     7441 tcgttccgat catcatgcgc gactgcacga tgccgacgat gccgccgggc tgcggctgcg
     7501 caacggactc gacgggcaag acgattctcg gcctgttcga tacgaacccg aaggactgcg
     7561 cggtgtcggt cgaggagatc aagaccaaca gcctcatcgt gtcgctgctc tcgccggacg
     7621 tgacgatcaa cggcaagatg gcgctgtcgc tcggcatcaa ggcgaccgcg gtcaagggca
     7681 cgtacacggc ccccaaccag taagcggcgg cctcaggcat caggcctcag gttccgcagg
     7741 gcgaagctct caggcgtggc tccaaggccg cgcctgtttc gttttcggcg cccggagcgc
     7801 aattcgcccg gccggcaact cggcttgccg ctcgcggcaa acgcgcggaa tcagcttgcg
     7861 gcggtcggca tagggtgtga agaacggcgg gcccatgaag cggttcgccc ttgtcgccct
     7921 ggtggctggc tgtgccggcg ccacgcccgt cgtgctgacg gggcgcgccg agacggcgtc
     7981 gtttccgggg cgcgtgacca tgcagctcgc cgcgtattcc gggccgagct cggtctgcga
     8041 cccgagcgac agcgcgaacc tgtgctccgc ggccaccgtg atcgcacgcg tcggtcacat
     8101 tctcgacggc gtacgtcgcg tgccgtgcag cgatgcggcc cgcgcgctcg accgctacgc
     8161 ggatctacac gcgcacgaga tcgcgacgct gttgcacctg cacgagctcg agccagcggc
     8221 ttcgctcgcg cggtgggagg accggcagcg tggcaccgcc gagctcgcgc tggtcgccgg
     8281 cttcgatctc gtcacgcgct gcgagcacga cgaccagctc gaacacgcgg tgcgccgggt
     8341 cggcctcggg agcttgttcg cgcgctctac ttctcgttga tgtgcggctc ggtcgcgagc
     8401 gggatcgaga gcgagacgaa cacgctgcgt ggcgcgccgt acgagctgcc gacgaagccg
     8461 ttgccgatgc gaaacgcata gcgccggtcg aacaggttct cgatgtcgat gccgaggcgg
     8521 atcggatact cgcgcgcgac gaacgtgtac tgcgacgaca gatcggcgac gaggtggcca
     8581 ggcacgtgct ggtcgttcgc cggcccggtg cgtagacccg agccgtacgt gaacatgccg
     8641 gtcacggtga agcggccatc gcgtaccgtc gcgccggcgt taccggtcca cgtctgcgcg
     8701 tggtcgagcg actgccaggt gttgtccgca aggtcgtcgg gggaaaacag aaacgtcgcc
     8761 gatgcgatgc cgcggccctg cgcgaatcca agcgatgcgt ttgcgaaccc tgacagccac
     8821 gggccgacgc gcagctcgag gctgccttcg aggccggcgg cacggccgcg tttgaagttg
     8881 tagttcgagt cgaggctcgt cgagccgatc gcggtgtcgt cgagctggtt gtacgagtag
     8941 cgtccccacg ccgtgaggcc gccgcggagc agcttgctga gccgcgcgag gatgccgacc
     9001 tcgctgtaga gatcggtctc cggtttcaga tcgtaggtga cgggttgatc ggcgggaacg
     9061 acgccgagcg cacgcgcggc actggccgca tcgagtggcg agggcggcag ccacatcacg
     9121 ccggagaaga cgtggccgac gacgtccttc gcgaacgagt acgagagccc gaggcgcggg
     9181 ctgacgccga acgagtcgtc cgtgccgcca ccttgcagca tcacgtgcaa ttgatcgatg
     9241 cgaatgccgg catccagcgc cgcgtcgccg agcgtgacgt gatcttggac gtacgcgccc
     9301 gatagcagcg cctcggtgcg atcgtgccct gacgtcgagc tggcgatgcc gccattcacg
     9361 tcgtcgcgtt cgtagagcgt gaagtgagac gagccgtaca aaaagtcggt ctgcaagccg
     9421 gacttcacga ggtggcgacc gttctgccac gagtacgccg cgataccgcc cgcgtgatgt
     9481 gcggtgcgct tgacgtcgct cgcggtcgag cctggatcgg cgccgtcacc gagcgcgtgc
     9541 tcggcatcgc cgacgagcgt gccgcgcgac agcttgtaca gcggcgcgag catgagctgg
     9601 ccgcgctgga gcttgtggat ccacgagaac gccgcgaacg cttcgtgctc gtgctcgcgc
     9661 gcgtcggtgt cgtgcgggat gaacgccggt gcttcgttgc cgaacttgtc gacggggcga
     9721 gggcgggtcg gctcgaatgg ccgcgcggcc ggatcgatcg gcacctcgaa gcgattgtgc
     9781 gcgtacatcg cgaacagctc gtagcggttg gactcgcacg gctgatagtc gaagcgggtg
     9841 aacacgcgcg cgctgagacc ggtgtcgtgc aggatcggat cgatcgacgg cgcatcgagt
     9901 gcgcgctgcg agtacgtcgc gctgccaccg atcaccatgc cggtgcgatc gctgagcttc
     9961 gccgagtaga caaggccggg ctcgacggtg tcgaacgagc cgtagcgcag gctgaggttg
    10021 ccctcgggat gctcggccgc gttgcgcgtg acgaggttga cgatcgcgcc gaggcgatag
    10081 ccgtactcgg ccgggatgcc gcccgtcatg atctcgagct gctgcacgag gcgaacggga
    10141 atcgacgcgg cgaacatgct gccgacggag tcgggcacgg ggatgccgtc gacctggtac
    10201 tggatgttgc cgtgctggcc gcggacgtag acctcgccga gtgagtcttc gacaaagcct
    10261 ggtgccttcg ccatcacgtc ggtgatcggc cggtcgtcgg caccgggcaa ctcggcgagc
    10321 gtctgccggc cgaccgccac gacgctgccg gtcgctttcg gtggtggagg cagcgcccag
    10381 tcctcctcga tgcggatcac ttcttcggta tcgacgagca cgagctcgac ggtgagcacc
    10441 tgtgacgacg tgatctgcag gtgctggtga tcctcgacga ggccgggcga cgacgcttcg
    10501 atcgtgtagt cgccgagcgg gatgccggtc agcgtgaagt gaccgtcctt atcggtgacg
    10561 gtcttgccga tcgtattg
//
LOCUS       DASUFX010000571        12929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_369961,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000571 DASUFX010000000
VERSION     DASUFX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369961"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFV99_22905"
     CDS             complement(<1..356)
                     /locus_tag="VFV99_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062246.1"
                     /translation="MGRPTGIAFVVALTGVAHADITVDPCACSPNKPGFHRASALTGD
                     WGGLRADLFDAGVKVQATYAGELFAAPGLESDRIVNAGLGALALDIDFATLAKIDHLG
                     SLHLAGFAIHGDGLDTR"
     gene            465..1721
                     /locus_tag="VFV99_22910"
     CDS             465..1721
                     /locus_tag="VFV99_22910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5062247.1"
                     /translation="MTSWSALVVDDDAGIRQSLRLCLEADGARVLGVGTAPAALEALD
                     RARFDLVLLDLWLGKESGLEAIPELLKRQPDLAIIVVTAFATFESAVEAMKRGASDYL
                     PKPFTPDQVRAATRRALESLRLRQRLADAEHQLEQAGATDERFDGESPAFRSLLKHAT
                     KAAAADAPVLVRGESGTGKTALARWLWKHSTRKDGPLITATAATVDDPRKLEQANGGT
                     LFIDEVGDLTPEGQSAVLRFLDERADVRLIVATSHNLEDDVRAGKFREDLLFRLNVVT
                     LQLPPLRERAEDVLALAHHFLARTAARQRRPVFELAESARRAIGTYTWPGNLRELRNA
                     IERAVISSPGPRIEVDDLGIPPAPEAASGQTGEVALGADVSIELLEREHIARIIARAP
                     TMEAAARTLGIDASTLQRKRKRYGLT"
     gene            1718..3529
                     /locus_tag="VFV99_22915"
     CDS             1718..3529
                     /locus_tag="VFV99_22915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062248.1"
                     /translation="MTRTPQLRTRLAIAGLLLIATTVAAGVWSVVAFRRVSAVVSDTV
                     TANQRITDTTSTLSQALEREDDAVVLVLNNSAAGRRELAAQRAAVDNALEQVIRQLET
                     PGVTTRLCADIDAYEQAVDELLAMAQEPNARTRYHEHVNPLLVRAVGTTDDISDAHFR
                     SSQQVAEWAGQQSTRSMQIVAAISIGALLLLMLNVLHFARVVMKPIGEMTRAVEEIRR
                     GDFSSRVTVYRDDELGRLGGGLNKMADELAEFKRTNIGEVIAAKDTLQATLEALPDAV
                     LVIGNDRKVSATNPRAVEALGPDSEQKLEELPVRAAVEDVLQSRAAPDATVDLAKAIE
                     LDVGGRKRRLLPRVVPIAGERGVVLVLSDVTELARLDEMRLELVAVASHELRTPLTTM
                     RMTLSMLQEHATSYVARDRELVTTAMMGVEQLSVLVDEFLDLTRIEAGELRLQMTRVS
                     VREIVDRATKAIGPACEQARVSLDIKYGELPSTITGDHARLAMVVSNLLSNALKYTQP
                     GDRIRVTASATTDTATIVVEDSGPGVPAEFRERVFERFFRVEHMQESALSSAGAGIGL
                     YIARQVIEAHGGTIRCSGSPLGGAQFTVTIPVDSTID"
     gene            complement(3526..5064)
                     /gene="mgtE"
                     /locus_tag="VFV99_22920"
     CDS             complement(3526..5064)
                     /gene="mgtE"
                     /locus_tag="VFV99_22920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5062249.1"
                     /translation="MSVTGDNVPEPVVDRPADATGEVSVLAPEEREKVAAEIRAVAES
                     RGDDSAVVELVEVGQTGRAKRPSIIPPMHGAPAPTDDDELSADELRDAWPLLDLDERA
                     DGLRVLPREEAEDFFVSLTPADQAALLLHFRAGQRRQWIRLLEPDDVVDMVQVAPEDH
                     REKLLALLDAPTRKEVMALLAYEEDEAGGLMSPRYARLRPQMTVGEAITYLQRQARDR
                     VETIYVAYVLDPEQHLLGVVSFRDLLTNDQKRPVSEIMETEVQRVTDEMDQEAVSRVF
                     AEHDLNVIPVVDRDGKMKGIVTVDDIVDVVREEATEDAQKFGGVEALDLPYLQSSRRL
                     MVRKRGRWLMILLIGEMLTATALGFFEKELIPVLALFLPLIISSGGNSGSQASTLIIR
                     AMALGEVRGRDWWLVMRREVLIGFALGALLGILAAVRVLIWGAAGTYGTHWVLIGVTV
                     AVSVTGCVLWGTLAGSMLPFVLRRFGADPANASAPLVATLVDVSGIIIYFTVVKLVLT
                     GVLI"
     gene            5174..6019
                     /locus_tag="VFV99_22925"
     CDS             5174..6019
                     /locus_tag="VFV99_22925"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX5062250.1"
                     /translation="MPADPAFLSQIQLFAQLDDDERSVLAQQMHERLLNAGEVLFRLG
                     EPGDSMFIVQSGAIELSVKDTAGQKIILHTATSGDFFGELSLLDEGSRTATATAVEGT
                     NLFVLDRDDLLQLFRKRPDAALDMLAAMGRMTRKANAMLRERVVKNVNEAVEVERGNI
                     VLRVADWVANFSGSITFLVLHVAIFTLWIGMNLTKFPFGEFDAYPFGLLTMSVSLEAI
                     ILSTLLLFSSNRQGARDRIRSDVEYDINLRAELEVAHLHEKTERLHEEMHERFNKLEK
                     ALRAR"
     gene            complement(6020..7324)
                     /locus_tag="VFV99_22930"
     CDS             complement(6020..7324)
                     /locus_tag="VFV99_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062251.1"
                     /translation="MMFLPGLAVIFAGLLGGHGDVDKQVANVLSDLHVKTVKLPAASL
                     QKLTHKDDETDDVVRSLDADGVIACEVVKGALRVWIYDHNGKLKTFSEVPLTKKGLTR
                     DAMDALRSNLGADIATMRGGKSEVADSPAPAKPAKPIVVAKREKPVAKPAAKQSEADV
                     VASDIDDDDPLAPKKAVRKPAPEPSPAAKETAAVDDAGSSDEIDALTAEQTDAKPAPA
                     TRSLRFGASAGIGAVSRSFKPGSSGVLRYSNTPVPAVALDAYVQPMRRLTLSIGGERS
                     LQLTTTMRDGTKVPTTISQWEAGGTYAFVHRPRVDLGVHVGAGGRAFAIDTMDPARSP
                     DGDYTYLIAGIDAAAHLGSRVTLRTIAAYEPVVSGTAPTEMASGDARGSALEVSGVIE
                     VRAASHVFVRAMAQYQRFSWSWDTGHASDQYPSGAIALGADY"
     gene            complement(7452..7943)
                     /locus_tag="VFV99_22935"
     CDS             complement(7452..7943)
                     /locus_tag="VFV99_22935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5062252.1"
                     /translation="MKSLSLLIVLALAATAHAEVKKGDHFVELDAKTATGKKFRLKDM
                     AGKWVLYTFGASWCEPCRKELPAWDKVAAKLAGKMLFVAVNINTDLDEGKQFVESLKL
                     RRIFPVFLPDENSPALKAYDPERMPSTFVIDPEGVVRIVRYGYHAGEEDKLAKELADL
                     VKK"
     gene            complement(7954..9396)
                     /locus_tag="VFV99_22940"
     CDS             complement(7954..9396)
                     /locus_tag="VFV99_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062253.1"
                     /translation="MMFLPGLAVIFAGLLGGHGDIDKQVTKVFRDLHVKAVKLPAASL
                     QKLTHHGEATRDLVRSLNADGVIACEVVKGESGSTLRIVIYEHDGRLKTFSEVPLTAK
                     GLSRDSLDVLRSNVGDDIASMQGTAPEPEPEIEIDPVPAKPAKPIVVAKPAKPEKPIV
                     VAKLDKPAAKPVKAKDEDHIVISDDVPTNAGVDDEDPLAGKKQNVRKPTAETKPAAEP
                     KPTAEPTVAATETAAADDAVSADEIAALTANIDDVTPVPAGRSLHFGASAGIGIASRS
                     FSPGPATVAAYSSAPVAAIVLDARIQPTQRLAIAIGTERSLQLSTPMSDGTNAATTIS
                     QWEAGGTYAFVQRPRFDLGARFGGGRRAFTIDSVDPARSPDSDYNYLTAGVDAAARVG
                     SRVTLRTTAAFEPVLFGAEPTEMAFGEASRWALELGAAVEVRATNHIFMRATAQYQRF
                     SWSWDMAGERGAGGAIDEYPSGALAIGADY"
     gene            complement(9393..9899)
                     /locus_tag="VFV99_22945"
     CDS             complement(9393..9899)
                     /locus_tag="VFV99_22945"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5062254.1"
                     /translation="MATCLGLTIAGCIDGDTGVIETGPLNGDQGSNGSGSGSNHPDAG
                     VITPTPDAGPAATCPTTGATDVGTPSTFNSNAPVYVSGGNFFVVRDAGGLYAVSARCT
                     HEGATCVIKTGQFYCPRHAAKFDFNGGVISGPVVTGLSHYAMCLLANGHVGVITSMKV
                     AKTDRLAA"
     gene            10101..10577
                     /locus_tag="VFV99_22950"
     CDS             10101..10577
                     /locus_tag="VFV99_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062255.1"
                     /translation="MLRNSWGWRIAMFAMLVGSACSVVENEPAADPAASLSEQVFRCN
                     VEPILVKQCSYNACHGIIGASFRVYSPGKLRSAVPADIDGAIAPLTDQEHHANFESAA
                     GFSFGITSVDDNWLLRKPLPSREGGYEHAGGAIWRGIGDPQYVAIRAWATNTGACK"
     gene            10574..11563
                     /locus_tag="VFV99_22955"
     CDS             10574..11563
                     /locus_tag="VFV99_22955"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEX5062256.1"
                     /translation="MKRVVFSLLVLSGTAHAFPTGSQFDLDALENDGGGGIAFDGAPR
                     FAGHTCAVCHTNAPEQIGSRIEADKVELFSEGWTPGQQYHLRVALLNAHAAAQYQAAG
                     DNCGGDTAATFKPCDQNGFALEIADRAGVPKGKFVPVVNNACANGMASTDVDVYVLED
                     GTAVTHSGAHHGQLTWDFCWTAPAAGAGLLTAYLSVVDGNGGMGTLAFPADTIGDDVT
                     SGAVPLPEVGGGAPPPQSGGCSTTEPGGSVLLALCVLVLVLRKRTRLALLLAFVVSAG
                     CTHVRPRQRETLARKNMKFAPDPTEDELDLHMQEAREGSSGGYGSSGGGCGCN"
     gene            11548..12612
                     /locus_tag="VFV99_22960"
     CDS             11548..12612
                     /locus_tag="VFV99_22960"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX5062257.1"
                     /translation="MRLQLILGLLAVAAVPARADTAFGSKLQVYTDNDHTTVVSPVVT
                     AEADVTTDTNVSIGYLVDAVSSASVDVVSQASPKTFHDTRHQVSLGMSHRFDTITPRI
                     GYSLSMEDDYLSHTLSLNATDELNDKNTQLGIGYGVSINRVGRAEDDNFSRPLTVHHV
                     AASWTQTMSPKVAGQLTYEISYSDGYQASPYRFVPIRQAADAVPEFWVPETDPDTRWR
                     HALVVGANAAVGDASSLQGDYRIYRDTWGITSHTLGARYFMSLAKHVELRLRERFYTQ
                     NAASFYQSVYMAPARYIVYDRELSPMWSETLGAKLTVGFGDRVEAELKLDGFYYSYAD
                     FVPLRSRTGANVGLGVSLAY"
     gene            complement(12614..>12929)
                     /locus_tag="VFV99_22965"
     CDS             complement(12614..>12929)
                     /locus_tag="VFV99_22965"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5062258.1"
                     /translation="VVVEASGGASGFELALDLLRPQGRLVLKSTFHGTTPLDAARIVV
                     EELEIIGSRCGRFAPALDLLARRAVDVRPLISDVMSLAHAEQALERAAAPGVLKVLLS
                     RA"
BASE COUNT         2224 a         4210 c         4243 g         2252 t
ORIGIN      
        1 cgcgtgtcga gaccgtcgcc atggatcgcg aagcccgcga ggtgtagcga gccgaggtga
       61 tcgatctttg cgagcgttgc gaagtcgatg tcgagcgcga gtgcgcccag gcccgcgttg
      121 acgatccgat cactctcgag gccgggcgcg gcgaacagct cgccggcata ggtggcttga
      181 actttgacgc cagcgtcgaa caggtcggct cggagaccgc cccagtcgcc ggtcagtgcg
      241 ctcgcgcgat gaaatccggg tttgttcgga ctacaggcac acggatcaac ggttatgtcg
      301 gcatgagcca cgcccgtcag agcgacgacg aacgcaatcc cagtaggtct tcccaccagg
      361 ctccatttca ccacgggtcc gcggggcatc tcgccccaag cccgagcaat ttgcacggac
      421 actcaggtgg acaaccgtcg acgagggtct acgctccgag acatatgacg agctggtcgg
      481 cacttgtcgt cgacgacgac gctggcattc gacagtcact gcgcctgtgc ctggaggccg
      541 atggtgctcg cgttctcggt gtcggcaccg cgccggcagc gctcgaggcg ctcgatcgcg
      601 cgcgtttcga tctcgtgttg ctcgatctgt ggctcggcaa ggagtcgggg ctcgaagcga
      661 tacccgagct cctcaagcgg caacctgatc tcgcgatcat cgtggtgact gcgttcgcaa
      721 cgttcgagtc cgcggtcgag gcgatgaagc gcggcgcgag cgactacctg ccgaagccgt
      781 tcacacccga tcaagttcga gccgcgacgc gccgtgcact cgagagcttg cgcttgcggc
      841 aacgccttgc cgatgccgag catcagctcg aacaggccgg cgcgaccgac gagcgcttcg
      901 atggtgagag cccggcgttc cgctcgctac tcaagcatgc gacgaaggcg gccgcagctg
      961 atgctcccgt gctcgtgcgc ggcgagagcg gcaccggcaa gacggcgctc gcgcgctggc
     1021 tatggaagca cagcacgcgc aaggatggtc cgctcatcac cgcgaccgcg gcgaccgtcg
     1081 acgatccgcg caagctcgaa caggcgaacg gcggcacgct gttcatcgac gaggttggcg
     1141 atctcacgcc cgaagggcag agtgcggtgc ttcgcttcct cgacgagcgt gcggacgttc
     1201 gcttgatcgt cgcgacgagt cacaacctcg aggacgacgt tcgtgccggc aagttccgcg
     1261 aggacttgct gttccgtctc aacgtcgtga cgttacagct gccgccacta cgcgagcgcg
     1321 ccgaggatgt gcttgcgctc gcgcatcact tcctcgctcg cactgcggct cgccagcgcc
     1381 gtccggtgtt cgagctggcc gaatccgctc gtcgtgcgat cggtacgtat acgtggccgg
     1441 gcaacctgcg cgagctgcgc aacgcgatcg agcgcgcggt gatctcgagc ccgggaccac
     1501 gcatcgaggt cgacgacctc ggaattccgc cggctcccga ggccgcgtcg ggtcagaccg
     1561 gcgaggtcgc gctcggcgct gatgtcagca tcgagttgct cgaacgcgag cacatcgcgc
     1621 gtatcatcgc gcgcgcgccc accatggaag cggccgcccg cactctcggc atcgacgcgt
     1681 ctaccctgca gcgcaagcga aagcgctacg ggctgacatg acgcggaccc cgcagctacg
     1741 tacacgtcta gcgatcgcgg gcctcttgct gatcgcgacc accgtagcag cgggtgtctg
     1801 gagcgtcgtc gcctttcgcc gggtgagcgc cgtcgtcagc gacacggtga ctgccaacca
     1861 acggatcacc gacacgacgt cgacgctgtc gcaggccctc gagcgtgagg acgatgccgt
     1921 cgtgctcgtg ctcaacaact cggcagcggg gcgacgcgag ctcgccgcgc agcgcgccgc
     1981 ggtcgacaac gcgctcgagc aagtcattcg ccagctcgag acgcctggcg tcacgacgcg
     2041 gctgtgcgcg gacatcgatg cttacgagca ggcggtcgac gagctgctcg cgatggcaca
     2101 agagccgaac gcgcgcacgc gctatcacga gcatgtcaac ccgctcctcg tccgcgcggt
     2161 cggaaccacc gatgacatct cggacgcaca ctttcggtcg agccagcagg tcgccgaatg
     2221 ggcgggtcaa caatcgacgc gctcgatgca gatcgtcgcc gcgatcagca tcggtgcctt
     2281 gctgctgctg atgctcaacg ttcttcactt cgcgcgcgtc gtgatgaaac cgatcggcga
     2341 gatgacccgc gcggtcgagg agattcgccg cggtgacttc tcgtcgcgcg tgaccgtgta
     2401 tcgcgatgac gagctcggtc gcctcggtgg cggcttgaac aaaatggccg acgagcttgc
     2461 cgagttcaag cgcacgaaca tcggcgaggt gatcgcggcg aaggacacgc tgcaggcgac
     2521 gctcgaagcg ctacccgatg cggttctggt gatcggtaac gatcggaagg tgtcggcgac
     2581 gaacccccgc gcggtcgaag cgctcgggcc agacagcgaa cagaagctcg aggagttgcc
     2641 ggtgcgcgct gcggtcgagg atgtcctgca atcgcgtgcc gcccccgatg ccacggtcga
     2701 tctcgcgaag gcgatcgagc tcgacgtcgg cggtcgcaag cggcggctgt tgccacgcgt
     2761 cgtgccgatt gccggcgagc gcggtgtcgt gctcgtgcta tcggacgtca ccgagctcgc
     2821 gcgactcgac gagatgcgac tcgagcttgt tgccgtcgcg tcacacgagc tgcgtacgcc
     2881 gctgacgacg atgcgcatga cgctgtcgat gctgcaggag cacgcgacga gctacgtcgc
     2941 acgtgatcgc gagctcgtca ccacggcgat gatgggcgtc gagcagctct cggtgctcgt
     3001 cgacgagttt ctcgatctga cgcgcatcga ggcgggtgag ctccggctcc agatgacgcg
     3061 cgtgtcggtg cgcgagattg tcgatcgcgc gaccaaggcg atcgggccgg cctgcgaaca
     3121 ggcgcgggtc tcgctcgata tcaagtacgg cgagctgccg tcgacgatca ccggcgatca
     3181 cgcgcgcctt gcgatggtcg tgtcaaatct actgtcgaat gcgctcaagt acacgcagcc
     3241 gggagaccgc atccgcgtga ccgcgtcggc gacgacggat actgccacga tcgtcgtcga
     3301 ggattcaggg ccgggcgtgc cggcggagtt ccgcgagcgc gtgttcgagc gcttctttcg
     3361 cgtcgagcac atgcaggagt ccgcgcttag ctcggcgggt gccggcatcg gtctctacat
     3421 cgcgcgccag gtgatcgaag cgcatggcgg cacgattcgc tgcagtggga gcccactcgg
     3481 cggcgcccag ttcacggtga cgattccggt cgactcgacg atcgactaga tcagcacgcc
     3541 agtcagcacg agcttgacga cggtgaagta gatgatgatg ccgctgacat cgacgagcgt
     3601 ggcgaccagc ggtgcggatg cgtttgcggg gtcggcgccg aaccggcgca gcacgaacgg
     3661 cagcatcgaa ccggcaagcg ttccccacag cacgcatccg gtgacactga ccgcgacggt
     3721 gacgccgatc agcacccagt gcgtgccgta ggtaccggcc gctccccaga tcagcacgcg
     3781 gaccgctgcg agaatgccga ggagcgcacc gagcgcgaat ccgatgagca cctcgcgccg
     3841 catgacgagc caccagtcgc ggccgcgtac ttcaccaagc gccattgcgc gaatgatcag
     3901 cgtcgacgct tgcgagcccg agttgccacc gctcgagatg atgagcggaa ggaacaacgc
     3961 cagcaccggg atgagctcct tctcgaagaa gccgagcgcg gtcgcggtca gcatctcgcc
     4021 gatcaagagg atcatcagcc agcggccacg cttgcgaacc atcagtcgac ggctcgactg
     4081 caggtagggc aggtcgagcg cttcgacacc accgaacttc tgggcgtctt cggtcgcctc
     4141 ttcgcgcacg acgtcgacga tgtcgtcgac ggtcacgatg cccttcatct tgccgtcgcg
     4201 atcgacgacg gggatcacgt tgaggtcgtg ctccgcgaag acgcgcgata ccgcctcctg
     4261 atccatctcg tcggtgacgc gctggacctc ggtctccatg atctcggaga ccggccgctt
     4321 ttgatcgttg gtcaacagat cgcgaaacga caccacaccg agcaggtgct gctcgggatc
     4381 gagcacgtat gcgacgtaga tcgtttcgac gcgatcgcgc gcttggcgct gcagataggt
     4441 gatcgcttcg ccgactgtca tctgcgggcg cagtcgggca tagcgcggtg acatgaggcc
     4501 gccggcctcg tcctcctcgt acgcgagcag cgccatgacc tctttgcgcg tcggtgcatc
     4561 gagcagcgcg agcaacttct cgcggtggtc ttcgggcgca acctggacca tatcgacgac
     4621 gtcgtcgggc tcgaggagcc gaatccattg ccggcgctgg ccggcgcgaa agtgcagcag
     4681 caacgcggct tgatccgcgg gcgtgagcga cacgaagaag tcctctgcct cctcgcgcgg
     4741 cagcacgcgc aagccgtctg ctcgctcgtc gagatcgagt agcggccacg catcgcgaag
     4801 ctcgtcggcg ctcagctcgt catcgtcggt cggcgccggc gcgccgtgca tcggcgggat
     4861 gatgctcggc cgcttcgcgc gtccggtttg accgacctcg acgagctcga cgacggccga
     4921 atcatcaccg cgactctcgg caacggcacg aatctcggcg gcgaccttct ctcgctcttc
     4981 gggcgcgagc acgctgacct cgccggtcgc gtcagccggc cgatcgacga ccggctcggg
     5041 tacgttgtcg ccggtaacag acatcaaaca caccgtgccc gatccccgca ggtccgtgaa
     5101 taccgtggct tcgggcaaat tgcccggaca cgttcggggc agctgtcggc gctgtcgcga
     5161 aatactgacc acgatgcccg ccgaccctgc gttcctgtcg cagatccaac tcttcgcgca
     5221 gctcgacgac gacgagcgca gcgtgctcgc gcagcagatg cacgagaggt tgctcaatgc
     5281 gggcgaggtg ctatttcgcc tcggcgaacc gggcgactcg atgttcatcg tccagagcgg
     5341 tgcgatcgag ctgtcggtca aggacaccgc gggtcagaag atcattctgc acaccgcgac
     5401 gtcaggcgat ttcttcggcg agctgtcgct actcgacgaa ggctcgcgca ccgcgacggc
     5461 gaccgcggtc gagggcacga atctgttcgt gctcgatcgc gacgacctgc tgcagctgtt
     5521 ccgcaagcgc cccgacgccg cgctcgacat gctcgcggcg atgggacgca tgacgcgcaa
     5581 ggcgaacgcg atgttgcgcg agcgtgtcgt gaagaacgtg aacgaagcgg tggaagtgga
     5641 gcgcggcaac atcgtgctgc gcgtcgccga ttgggtcgcg aacttctccg gcagcatcac
     5701 gtttctcgtg ctgcacgtcg ccatcttcac gctgtggatc gggatgaacc tcaccaagtt
     5761 cccgttcggt gagttcgatg cgtacccgtt cggcctgctc acgatgtcgg tgtcgctcga
     5821 agcgatcatc ttgtcgacgc tgctgctgtt cagctcgaac cggcaaggcg cgcgcgaccg
     5881 cattcgcagc gacgtcgagt acgacatcaa cctcagggcc gagctcgagg tcgcgcacct
     5941 gcacgagaag accgagcgtc tccacgagga gatgcacgaa cgcttcaaca agctcgaaaa
     6001 ggcgctgcgc gcgcgctgac tagtagtcgg cgcccagtgc gatcgcgccc gatgggtact
     6061 ggtcgctcgc gtggcccgtg tcccaggacc acgagaaccg ctgatactgg gccatggccc
     6121 gtacgaagac gtggctcgcg gcgcgtacct cgatcacgcc actgacctcg agtgccgagc
     6181 ctcgtgcatc gccggatgcc atctcggtcg gtgccgtgcc ggacaccacc ggctcgtacg
     6241 cagcaatggt gcgcagcgtg acgcgtgagc caaggtgcgc ggcggcatcg atgcccgcga
     6301 tcaggtacgt gtaatcgccg tcgggtgagc gggctggatc catcgtatcg atcgcgaagg
     6361 cgcgcccgcc ggcgccaacg tgcacaccga ggtcaacccg cggacggtga acgaatgcgt
     6421 acgtgccacc ggcttcccac tgcgagatcg tcgtcggcac cttcgtgcca tcgcgcatcg
     6481 tcgtggtcag ctgaaggctg cgctcgccgc cgatcgaaag cgtgaggcgt cgcatcggtt
     6541 ggacataggc gtcgagcgcg accgccggga ccggcgtatt cgaataccga aggacgcccg
     6601 acgacccggg cttgaacgag cggctcacgg cgccgatgcc agcagacgcg ccgaaacgca
     6661 gcgagcgcgt ggcgggcgca ggcttcgcgt cggtctgttc tgctgtcagc gcatcgatct
     6721 cgtcgctgct acccgcatcg tcgacggctg cggtttcctt cgcggccggc gagggctcgg
     6781 gcgccggctt gcgcacggcc ttcttgggcg ccagtggatc gtcgtcgtcg atatcgctgg
     6841 cgacgacgtc agcctcggat tgtttggctg ccggctttgc aaccggcttc tctcgctttg
     6901 cgacgacgat cggtttggcc ggtttcgccg gggctggact gtcggcgacc tctgacttgc
     6961 cgcctcgcat cgtcgcgatg tcagcgccga ggttcgagcg cagcgcgtcc atcgcatcgc
     7021 gcgtgagccc cttcttggtg agcggcacct cgctgaacgt cttgagcttg ccgttgtggt
     7081 cgtagatcca gacgcgcagc gctcccttga cgacctcgca tgcgatcacg ccgtccgcat
     7141 cgaggctgcg cacgacatcg tccgtttcat cgtctttgtg cgtgagcttc tgcaaggacg
     7201 ccgcgggcag cttgacggtt ttgacatgca gatcgctgag cacgttcgcg acctgtttgt
     7261 cgacgtcacc atgccccccg aggaggccgg caaagatcac ggcgagtccg ggcaagaaca
     7321 tcatgcgacg aggccgtcag ctcccttcat gctgcgtact gaaacaaaaa cgaccgcgac
     7381 tgtcccacgt gtcccactca cacgggctca cggacctgac gatgccgagc atcgtgtgaa
     7441 cgcgaaatac cctacttctt cacgagatcg gcaagctctt tggcgagctt gtcttcttcg
     7501 ccggcgtggt agccgtagcg cacgattcgc acgacgcctt caggatcgat cacgaacgtc
     7561 gacggcatgc gctccgggtc atacgccttc agcgccgggc tattctcgtc gggcaagaac
     7621 accgggaaga tccggcgcag cttcagtgac tcgacgaact gcttgccctc gtcgaggtcg
     7681 gtgttgatgt tgacggcgac gaacagcatc ttgccggcga gcttcgcggc gaccttgtcc
     7741 caggccggca gctctttgcg gcagggctcg caccagctgg cgccaaacgt gtagagcacc
     7801 cacttgccgg ccatgtcctt gaggcgaaac ttcttgccgg tggcggtctt cgcgtcgagc
     7861 tcgacgaagt gatcgccctt cttcacctcc gcgtgagcgg tggcggcgag cgcgagcacg
     7921 atcagcaggg acaaggactt catggcggac tcctcagtaa tcggcgccga ttgcgagcgc
     7981 gccggatggg tattcgtcga tcgcgccccc tgcaccgcgt tcacccgcca tgtcccacga
     8041 ccacgagaac cgttggtatt gcgccgtcgc gcgcatgaag atgtgattcg tcgcccgcac
     8101 ctcgacagcc gcgccgagct cgagcgccca gcgcgacgct tcaccgaatg ccatctcggt
     8161 cggctcggca ccgaacagga ccggctcgaa cgcggcggtc gtgcgcagcg tcacgcgtga
     8221 cccgacgcgt gcggcagcat cgacgccggc ggtgagatag ttgtaatcgc tgtcgggtga
     8281 gcgcgctggg tcgaccgaat cgatcgtgaa cgcgcggcgg ccgccgccga accgcgcgcc
     8341 gaggtcaaac cgaggacgtt ggacgaacgc atacgtgccg ccggcttccc attgcgagat
     8401 cgtggttgcg gcgttcgtgc cgtcactcat cggggtcgag agctggaggc tgcgttcggt
     8461 gccgatcgcg atcgcgaggc gttgcgtcgg ctggatgcga gcatcgagca cgatcgccgc
     8521 gaccggtgca ctcgagtacg cggcgaccgt cgctggtccg gggctgaacg agcggctcgc
     8581 aatgccgatt cccgccgacg caccgaagtg cagggaccgt ccggcaggga cgggcgtcac
     8641 gtcatcgatg ttcgcggtta gcgctgcgat ctcgtcggcg ctgaccgcat catcggcggc
     8701 tgcggtttcg gtcgctgcaa ccgtgggctc ggcggttggc ttgggctctg ccgccggctt
     8761 ggtctccgcc gtcggctttc gcacgttctg tttcttgccg gcgagtggat cttcgtcatc
     8821 gacgccggcg ttcgtgggca cgtcgtcgct gatgacgatg tggtcctcgt ccttggcctt
     8881 cactggcttc gccgccggct tgtcgagctt cgcgacgacg atcggcttct ccggtttcgc
     8941 cggcttcgca acgacgattg gcttcgccgg tttcgctgga accggatcga tctcgatctc
     9001 tggctccggc tccggcgcgg tgccctgcat cgaggcaata tcgtcgccga cgttcgagcg
     9061 gagcacgtcg agcgagtcgc gggagagacc cttcgcggtg agcggcacct cgctgaacgt
     9121 cttgagccgg ccatcgtgtt cgtagatcac gatgcggagc gtcgacccgc tctcgccttt
     9181 gacgacctcg cacgcgatca cgccatcggc gttcaagctg cgcacgagat cgcgggtcgc
     9241 ttcgccgtga tgggtgagct tctgcagcga cgctgcaggg agcttgaccg ctttgacatg
     9301 cagatcgcgg aacaccttcg tgacctgctt gtcgatgtca ccatgacccc cgagaaggcc
     9361 ggcaaagatc acggcgagtc cggggaggaa catcacgcgg ccagccgatc ggtcttcgcg
     9421 accttcatgg aggtgatgac gccgacgtgg ccattcgcga gcagacacat cgcgtagtga
     9481 gacaggccag tgacgaccgg gcccgagata actccaccat tgaagtcgaa cttcgcagca
     9541 tgacgcgggc agtagaactg gccggtcttg atgacgcagg tcgcaccctc gtgcgtacag
     9601 cgcgcactca ctgcatagag cccaccggca tcgcgcacga cgaagaagtt gccgccgctg
     9661 acgtacaccg gagcattcga gttgaacgtc gacggcgtgc ccacgtccgt cgcacccgtg
     9721 gtggggcagg tggctgcagg tcctgcgtcc ggtgtcggcg tgatcacacc cgcatcgggg
     9781 tggttggaac cggaaccgga accgttcgag ccctgatcgc cgttgagcgg cccggtctcg
     9841 atcacaccgg tatctccatc gatgcagcct gcgatggtca aaccaagaca ggtcgccacg
     9901 ccgccacaga attgacgacg tgagagcgca acaaggccct cggctcttct catcgagggg
     9961 tactgaacca aaacgagccc gggctgtccg ccgtatcctg cttacacggg ctcacgcagg
    10021 tgacgatgcc aagcatcgcc gcaatgtgaa ggacaaatac ctgatgcggc cgacgacaaa
    10081 cttggctagc gtctccgccg atgttgcgca acagctgggg ctggcgcatc gcgatgttcg
    10141 cgatgctcgt cggcagcgcg tgctcggtcg tcgagaacga gcccgccgcc gatcccgcgg
    10201 cttcactctc cgagcaggta tttcgctgca acgtcgagcc cattctggtg aaacagtgca
    10261 gttacaacgc gtgccacggc atcatcggtg cgtcgtttcg cgtgtactcg ccgggtaagc
    10321 tgcggtctgc tgtccccgcc gacatcgacg gcgcgatcgc gccgctcacc gatcaagagc
    10381 accacgcgaa ctttgaatcg gctgcgggct tttcgttcgg gatcacgtcg gttgatgaca
    10441 actggttgct acgcaagcca ttgccatcac gtgaaggtgg gtacgagcac gcgggcggtg
    10501 cgatctggag aggaattggc gatccgcaat acgttgcgat tcgcgcatgg gcgacaaaca
    10561 cgggagcctg caaatgaagc gggtcgtgtt ctcgctgctc gtgctctcgg gaacggctca
    10621 cgcgtttccg actggctcgc agttcgatct cgacgcgctc gagaacgatg gcggcggtgg
    10681 gatcgcgttc gatggtgcac cgcgcttcgc gggtcacact tgcgcggtct gccacacgaa
    10741 tgcgccggaa cagatcggct cgcggatcga agccgataaa gtcgagttgt tcagcgaggg
    10801 ctggacaccg ggccagcagt atcacctgcg cgttgcgcta ctgaacgcgc acgcggctgc
    10861 tcagtatcaa gctgcaggtg acaactgtgg cggcgatacg gctgccacgt tcaagccatg
    10921 cgatcagaat ggctttgcac tcgagatcgc agatcgcgcg ggcgtgccga aaggcaagtt
    10981 cgtgccagtc gtcaacaacg cctgcgcgaa tggaatggcg tcgaccgatg tcgacgtcta
    11041 cgtcctcgag gacggcaccg ccgtcacgca tagcggtgcg caccacggtc agctcacgtg
    11101 ggacttctgt tggaccgcac ccgcggccgg tgccggcctc ctcaccgcgt atctctccgt
    11161 cgtcgatggc aacggcggta tgggcacgct ggcatttccg gccgacacga tcggcgacga
    11221 cgtcacgtcg ggggccgtcc cactgcccga ggtcggcggt ggagcgcctc cgccgcaaag
    11281 cggtggttgc tcgacgaccg agccgggcgg cagcgtgctg ctcgcgctgt gtgtgctcgt
    11341 gctcgtcctc cgcaagcgca cgcggctcgc gttgctgctc gcattcgtcg tgtcagcggg
    11401 ctgcacgcac gtgcggccgc gccagcgcga gacgctcgcg cgcaagaaca tgaagttcgc
    11461 gcccgatccg acggaagacg agctcgatct ccacatgcag gaggcgcgcg aaggctcgtc
    11521 gggtggctac ggttcgagcg gtggtggatg cggctgcaac tgatcctcgg gttgctcgca
    11581 gtcgccgccg tgcccgcgcg cgcggatacc gcctttgggt ccaagctgca ggtctatacc
    11641 gataacgatc acaccacggt ggtgtcgccg gtcgtcaccg cagaggccga cgtcacgacc
    11701 gacacgaacg tatcgatcgg ctacctcgtc gatgcagtga gctcggcatc ggtcgacgtc
    11761 gtgtcgcaag catcgccgaa aacgtttcac gacacgcgcc accaagtctc gctcggcatg
    11821 tcgcacaggt tcgacaccat cacgccgcgg atcggctact cgctgtcgat ggaggacgac
    11881 tatctctcgc acacgctgtc gctgaacgcg accgacgagc tgaacgacaa gaacacgcag
    11941 ctcggcatcg gctacggcgt gtcgatcaat cgcgtcggac gcgcggagga cgacaacttc
    12001 tcgcggcccc tcaccgtgca tcacgtcgcc gcgtcgtgga cgcagacgat gtcgccgaag
    12061 gtcgccggtc agctgaccta cgagatcagc tactccgatg gctatcaagc aagcccgtat
    12121 cggttcgtgc cgattcgcca agcggcagat gcggtgccgg agttctgggt gccggagacc
    12181 gatccggata cgcgctggcg gcacgcgctc gtcgtcggcg ccaatgcggc ggtcggcgac
    12241 gcaagctcgt tacaaggcga ctaccggatc tatcgggaca cgtggggcat cacgagtcac
    12301 acgctcggtg cgcgttactt catgagcctc gcgaagcacg tcgagctgcg gttgcgcgag
    12361 cgcttctata cgcagaacgc cgcgtcgttc tatcagagcg tgtacatggc gccggcgagg
    12421 tacatcgtct acgaccgcga gctatcgccg atgtggtcgg agacgctcgg cgccaagctc
    12481 actgtcggat tcggcgatcg cgtcgaggct gagctcaagc tcgacggctt ctactacagc
    12541 tacgcggact tcgtgccgct gcgctcgcga accggcgcga acgtcggcct cggcgtctcg
    12601 ctcgcgtatt gagctaggcg cgggagagca ggaccttgag cacgccgggc gcagcggcgc
    12661 gctcgagcgc ttgctccgcg tgcgcgagcg acatgacgtc gctgatcagt ggccgtacat
    12721 cgacggcgcg ccgagcgaga agatcgagcg ccggcgcaaa ccggccgcag cgcgagccga
    12781 tgatctcgag ctcctcgacg acgatccggg ccgcatcgag cggtgtcgtg ccatggaacg
    12841 tcgatttgag gacgagcctg ccctgtgggc gcagcagatc gagcgccagc tcgaagccac
    12901 tcgcgcctcc cgacgcctcg accacgacg
//
LOCUS       DASUFX010000572         6374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_370257,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000572 DASUFX010000000
VERSION     DASUFX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370257"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VFV99_22970"
     CDS             <1..1356
                     /locus_tag="VFV99_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062259.1"
                     /translation="GWTVYGPAGSTSQSTNVKSVSDGIWEPTQASCTEAATGAAELHP
                     PPPNIIGGYSVQLRAEWAIELPALYSRFIASFANRPIQPPNSSKKYCAIRKQGTTARV
                     VCLDNGIARDYEVVVAGGQVTLDQRDMMVLGPDALDVVSVPSGADRDVYMMSTKGVLV
                     PLFGAPPADNSGAPCTSGSCEVDDVIAVPACGSLPGKLIVRLKATGSGQLLQMNARGG
                     GTQEFPIGTLAIGVQAKLDNAGCVTRADPGGGPPTLRQVVTYHLGSPNPLGEFVAVAT
                     RAAYGCTATTCMSNELFPGAGVAFTSGDEQRMLITFVDASGVVIAELIMAPDKPMKDL
                     FVERSRSPAASIPDRLVVGQYDADTDTDMFWNMGARRGTTFEVGYGRKVGTQRLEALS
                     AIQPITVTGLDSADLTGDGRDDVMVIGDVIGTTASGLVVIPMGAGAPTQQIASDATCS
                     P"
     gene            1438..1884
                     /locus_tag="VFV99_22975"
     CDS             1438..1884
                     /locus_tag="VFV99_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062260.1"
                     /translation="MLRWLAILAISCGHPSTPAKPQGGSSPTPPADAAVDAPIGLESD
                     LPRLVERAVKMFSDMQAALEAAGEDCAAATAKLTAIADANADVMEANTKVLRAGRDKM
                     KAMREEIAKHQAELDASGAAIASSPTMKKCSSDPAFAKVVDRLGGE"
     gene            1881..3497
                     /locus_tag="VFV99_22980"
     CDS             1881..3497
                     /locus_tag="VFV99_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062261.1"
                     /translation="MTRPRLSAAALLVGLWLAPMIAFAQPFVPGTQSSDKSLPAPISL
                     PVPMHNGSNTSAGSAVGSGSAETPKDADAHSTEATKHNTLFAIKVIAGLMVLLALAYL
                     GGHRKVVRFQERLGISGVVTAGFPFVAMGAIASHPDVGILSGDVVSRLRPILHFGLGW
                     LGFIIGAQLDIRVLDRVPKGTAYLIFVEALGPFAITAAGCGALMISFGMSISDPATWR
                     DMILLGTAAAMTAPRKFRGFANRTWHEGRGVDVLLGQLDEMVGVIGLVFITAYFREDT
                     NVTWQLPSTAWVFVSLGLGVAIGVLIFAMIRVPQSNVEFLAIVLGGIAFASGLAGYLR
                     LSPIAISFIAGVLVTNFPNDQRDSVFRILHHLERPVHLLFLIVAGAVWDISDWRGWVL
                     IPLFVAGRVLGKWAGVMTAKTAVGQFLPENFADTRNWVTPLSGLSIALVISVQSLYQD
                     AGLAWIVTAVIGGSIVTEFLVSRTSTTVFRPITNPALPTVPAHPVDELDHGGHLEDLD
                     DLDDERDAPDGPIYRDESPAAPAKKPGGGH"
     gene            3497..4825
                     /locus_tag="VFV99_22985"
     CDS             3497..4825
                     /locus_tag="VFV99_22985"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5062262.1"
                     /translation="MIHLLLLIAVGGMMQAARSFTTDTSGAGTELGFGFLLLASYFTA
                     KIFNRFGLPKLTGYIAAGVIAGPYVLEFVTKDMGVSLKVVSTTATAILALEAGSELEI
                     KQLRPILKTLRGITLFAVVGSMFTIATALFLMRPMFPSIFDKLDTQQSIAVCFAIGVA
                     LSAQSPAVVMAMLAETRAEGPLSRTMLASVVVADLTVITLFSIVLAITGAVIGGQVDV
                     AGTAISVAWELLGSMVFGLLLGALIGQFLLSVKKGASMFTLLVNVVVAEIGARVHLDP
                     LIVMLTAGIWLRNWSRADSTKMMHEFQAAQLPVFLVFFALAGSKLDINVLFTSLLPVA
                     IIAAVRASSFFMGAKIACKVSGAEEVISKYAWFGLVPQAGLALALALVLKNTLPSFGF
                     DQSAGDTAAVILFGVVGFNECIAPIILRIMLIRSGEAGKKKGVDFAAGGH"
     gene            complement(4866..5777)
                     /locus_tag="VFV99_22990"
     CDS             complement(4866..5777)
                     /locus_tag="VFV99_22990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX5062263.1"
                     /translation="MAKPILFEPVEGPHLVPFDGDFKLKKVSTKPPDDAPGKKENVRE
                     LDEVVDKLTELQAKLYAQDRYSVILIFQALDAAGKDGTIKAVMSGVNPQGCQVTAFKQ
                     PSAEELDHDFLWRVELALPERGRIGIFNRSHYEEVLVVRVHPEYLEAQRLPRVPKDLD
                     ELWVERYDSINEAEKHWARNGAVILKFFLHVSQEEQHRRFLERILDPDNNWKFSPGDM
                     AESEKWDEYMEAYEDMLRATSKPWAPWYIIPADKKAYMRRVVGEIVVETLKRLPLAFP
                     KVSDKQRAAMDGYRRELEALERKNRSD"
     gene            5887..>6374
                     /locus_tag="VFV99_22995"
     CDS             5887..>6374
                     /locus_tag="VFV99_22995"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5062264.1"
                     /translation="MEAIRYAESDKLGPLGTKRSILEDGIELRRMLVAEFQSAADAAR
                     DAASRVDKDAAAAVHDYRKALRRARAVLALAAGALPRSERRAILRALREARRALSPAR
                     DHAVVPDTVASVELDEVDRSTAATIISSTADSAPPQAEIKQLLAEGAARAAAQVEALE
                     AS"
BASE COUNT         1036 a         2155 c         2050 g         1133 t
ORIGIN      
        1 ggttggaccg tgtacgggcc cgccggctcg acctcgcaat cgacgaacgt gaagtcggtg
       61 agcgatggca tctgggagcc gacgcaagcg tcgtgcaccg aagccgcgac gggcgccgcc
      121 gagcttcatc caccgccacc gaacatcatc ggaggctact cggtgcagct gcgcgcggaa
      181 tgggcgatcg agctgcctgc gctgtactcg cgtttcatcg ccagcttcgc gaaccgaccg
      241 atccagccgc cgaacagctc gaagaagtac tgcgcgattc gcaagcaggg cacgaccgca
      301 cgcgtcgtct gcctcgacaa cggcatcgcg cgcgactacg aggtggtggt cgccggcggc
      361 caggtcacgc tcgaccagcg cgacatgatg gtgctcggtc cggacgcact cgacgtggtc
      421 tcggtgccga gcggcgccga tcgcgacgtc tatatgatgt cgacgaaggg tgtgctcgtg
      481 ccgctgttcg gcgcgccacc cgccgacaac agcggtgcgc cgtgcacgag tggctcgtgc
      541 gaggtcgacg acgtgatcgc cgtacctgca tgcggctcgc tgcccggcaa gctgatcgtg
      601 cggctcaagg cgaccggctc gggccaactg ctccagatga acgcgcgcgg cggtgggacg
      661 caggagtttc cgatcggcac gctcgcgatc ggggtgcaag ccaagctcga caacgcgggc
      721 tgcgtcacgc gcgcggatcc gggtggcggc ccaccgacgc tgcgtcaggt cgtcacgtat
      781 cacctcggtt cgcccaatcc gctcggcgaa ttcgttgcgg tcgcgacgcg tgccgcgtac
      841 ggctgcacgg cgacgacgtg catgagcaac gagctgttcc ctggtgcagg cgtcgcgttc
      901 acgagtggcg acgagcagcg catgctgatc acgttcgtcg acgcgagcgg cgtcgtcatc
      961 gccgagctga tcatggcgcc cgacaagccg atgaaggatc tgttcgtcga gcgctcgcgg
     1021 tcaccggcgg caagcattcc cgatcgactc gtcgtcggcc aatacgatgc cgacaccgac
     1081 acagacatgt tctggaacat gggcgcgcgg cgcggcacga cattcgaggt cggctacggc
     1141 cgcaaggtcg gcacgcaacg gctcgaggcg ctctccgcga tacagccgat caccgtgacg
     1201 ggcctcgata gcgccgacct caccggcgac ggccgcgatg acgtgatggt gatcggcgac
     1261 gtcatcggca cgaccgcctc tggacttgtc gtgattccaa tgggggccgg tgcgccaacg
     1321 cagcagattg caagcgacgc gacctgctcc ccttgaccac acgttcagaa agatccggag
     1381 tgaactaacg gcgccggtcg cgcgaccgcc gaggcgagtt gtgtgacgat gatcgcgatg
     1441 ctgaggtggc tcgcgattct cgcgatcagc tgtggccacc cgagcacgcc tgccaagccg
     1501 caaggcggga gcagcccgac gccgccagcc gatgctgccg tcgatgcgcc catcgggctc
     1561 gagagcgatc tgccacgtct cgtcgagcga gcggtgaaga tgttcagcga tatgcaggct
     1621 gcgctcgaag ccgccggtga ggactgcgcc gccgcaacgg cgaagctcac cgcgatcgcc
     1681 gacgcgaacg ccgacgtcat ggaagccaat acgaaggtgc tgcgcgccgg ccgcgacaag
     1741 atgaaagcga tgcgcgaaga gatcgcgaag catcaagcgg agctcgatgc gagcggcgca
     1801 gcaatcgcga gctcaccgac gatgaagaag tgctccagcg atccggcgtt cgcgaaggtc
     1861 gtcgatcggc tcggtggtga atgacgaggc cgcgcctgtc cgctgctgcg ctactcgtcg
     1921 gcctgtggct ggcgccgatg atcgccttcg cgcagccatt cgtgcccggc acgcaaagca
     1981 gcgacaagtc cctgcccgct ccgatctcgc tgccggtgcc gatgcacaac ggctccaata
     2041 cgagcgccgg ctcggcggtc ggatccggat cggccgagac gccgaaggac gccgacgcgc
     2101 actcgacgga ggcaacgaag cacaacacgc tgttcgcgat caaagtcatt gcgggcctga
     2161 tggtgctgct cgcgctcgcg tatctcggcg gtcaccgcaa agtcgtgcgc ttccaggagc
     2221 gactcggtat cagcggcgtc gtgaccgcag gcttcccgtt cgttgcaatg ggagcgatcg
     2281 cgagccatcc cgatgtcggg atcctgagcg gagacgtcgt cagtcgattg cgaccgatct
     2341 tgcacttcgg tctcggctgg ctcggcttca tcatcggtgc gcagctcgac atccgtgtgc
     2401 tcgatcgcgt gccgaagggc accgcgtatc tgatcttcgt cgaggcgctc ggcccgttcg
     2461 cgatcaccgc cgccggttgc ggcgcgctga tgatctcgtt tggcatgtcg atcagcgatc
     2521 cggcgacgtg gcgcgacatg atcctgctcg gcaccgccgc cgcgatgacc gcgccgcgca
     2581 agttccgcgg cttcgcgaac cgcacctggc acgaaggccg cggtgtcgac gtgctgctcg
     2641 gccagctcga cgagatggtc ggcgtgatcg gcctcgtgtt catcaccgcg tacttccgcg
     2701 aggacacgaa cgtcacgtgg cagctgccgt cgaccgcgtg ggtgttcgtg tcactcggtc
     2761 tcggcgtcgc catcggcgtg ctcatcttcg cgatgatccg cgtgccgcaa tcgaacgtgg
     2821 agttcctcgc gatcgtcctc ggcggcatcg cgttcgcgtc gggtctcgcc ggctatttgc
     2881 ggctctcgcc gatcgcgatc tcgttcatcg caggcgtgct cgttacgaac ttcccgaacg
     2941 atcaacgcga cagcgtgttc cgcatcctcc atcacctcga gcggccagta cacctgctgt
     3001 tcctgatcgt cgccggtgcc gtctgggaca tctccgactg gcgcggctgg gtcctgatcc
     3061 cgctgttcgt cgccggccgc gtcctcggca agtgggccgg cgtgatgacc gcgaagaccg
     3121 ccgtcggcca gttcctcccc gagaacttcg ccgatacccg caactgggtc acgccgctgt
     3181 cgggcctctc gatcgcactc gtgatctcgg tccagagcct ctatcaggat gccggcctcg
     3241 cctggatcgt caccgccgtg atcggcggct cgatcgtcac cgagttcctc gtgtcgcgga
     3301 cgtcgacgac cgtgttccgc ccgatcacga atcctgcgct gccgacggtg ccggctcacc
     3361 cggtcgacga gctcgatcac ggcggccacc tcgaagatct cgacgatctc gacgacgaac
     3421 gtgacgcgcc cgacggtccg atctaccgcg acgaatcgcc cgcggcgccg gcaaagaagc
     3481 caggaggagg ccactaatga tccacctcct gctcctcatc gcggtcggcg gcatgatgca
     3541 agccgcgcgc agcttcacga ccgacaccag cggcgccggc accgagctcg gcttcggctt
     3601 cttgctgctc gcgtcgtact tcacggcgaa gatcttcaac cgcttcggcc tcccgaagct
     3661 gaccggctac atcgcggctg gcgtgatcgc cggtccctac gtgctcgagt tcgtcaccaa
     3721 ggacatgggc gtctcgctca aggttgtcag cacgacggca accgccatcc tcgcactcga
     3781 agccggctcc gagctcgaga tcaagcagct caggcccatt ctgaagacac tgcgcggcat
     3841 cacgctgttc gcggtcgtcg gctcgatgtt cacgatcgcg actgccctgt tcctcatgcg
     3901 gccgatgttc ccgtcgatct tcgacaagct cgacacccag cagagcatcg ccgtgtgctt
     3961 cgcgatcggc gtcgcgctct ctgcgcagtc acctgcggtc gtgatggcga tgctcgccga
     4021 gacacgcgcc gaaggtccgc tcagtcgcac gatgctcgcg tcggtggtcg tggccgacct
     4081 caccgtgatc accctgttct ccatcgtgct cgcgatcacc ggtgcggtga tcggtggtca
     4141 ggtcgatgtc gccggcaccg ctatctcggt cgcctgggag ctgcttggtt cgatggtgtt
     4201 cggcctgctg ctcggcgcgc tgatcggcca gttcctgctg tcggtgaaga agggagcgtc
     4261 gatgttcacg ctcctcgtca acgtcgtcgt cgccgagatc ggcgcgcgcg tgcacctcga
     4321 tccactgatc gtcatgctca cggcgggcat ctggctccgc aactggtcgc gcgctgactc
     4381 gacgaagatg atgcacgagt tccaggccgc gcagctgccg gtcttcctcg tgttcttcgc
     4441 gctcgccggc agcaagctcg acatcaacgt gctgttcacg tcgctactgc cggtcgccat
     4501 catcgcggcg gtgcgcgcga gcagcttctt catgggcgcg aagatcgcct gcaaggtaag
     4561 cggcgccgaa gaagtcatct cgaagtacgc gtggttcggc ctcgtgccac aagctggcct
     4621 cgcactcgca ctcgcgctgg tgctcaagaa cacgttgccg tcgttcggct tcgatcagtc
     4681 cgccggcgat accgctgcgg tgatcttgtt cggtgtcgtc ggcttcaacg agtgcatcgc
     4741 gccgatcatc ctgcgcatca tgctcatccg cagtggcgag gccggcaaga agaagggcgt
     4801 cgacttcgcg gccggtggcc actgacgggc ttcggacctc aggcttcgga cttcagccag
     4861 aacaactaat cggatcgatt ttttcgctcg agcgcctcga gctcgcgccg gtagccatcc
     4921 atcgccgcgc gctgcttgtc ggagactttc gggaacgcga gcggtagccg cttcagcgtc
     4981 tcgacgacga tctcgccgac gacgcgccgc atgtacgcct tcttgtcggc aggaatgatg
     5041 taccagggcg cccacggctt gctcgtcgcg cggagcatgt cctcgtacgc ttccatgtac
     5101 tcgtcccact tctcgctctc ggccatgtcg cccggcgaga acttccagtt gttatccgga
     5161 tcgaggatgc gctcgagaaa ccgccggtgc tgctcctcct gcgagacgtg gaggaagaac
     5221 ttcagaatca cggcgccgtt gcgcgcccag tgcttctcgg cctcgttgat cgagtcgtag
     5281 cgttcgaccc acagctcgtc caagtccttc ggtacgcgcg gcaagcgctg agcctcgagg
     5341 tactcggggt gcacgcgcac gacgaggacc tcctcgtagt ggctacggtt gaagatgccg
     5401 atgcggccgc gctcggggag cgcgagctcc acacgccaca gaaaatcgtg atcgagctcc
     5461 tcggctgacg gctgcttgaa cgcggtgacc tggcagccct gcggattgac gccgctcatc
     5521 accgccttga tcgtgccgtc cttgcccgcg gcatcgagtg cctgaaagat caggatcacc
     5581 gagtagcgat cctgcgcgta cagcttcgcc tgcagctcgg tgagcttgtc gacgacctcg
     5641 tcgagctccc tcacgttctc cttcttgccg ggagcgtcgt cgggtggctt cgtcgacact
     5701 ttcttgagct tgaagtcgcc gtcgaacggc acgaggtgcg ggccctcgac gggctcgaac
     5761 aagatcggct tggccatcgc gcgaacgata cgaagattcg atttcgcgtg tcggtaatcc
     5821 ggacctgttc cggtaaggtg cctggtgatg gcgacgacga cgagctccga ggggaacggt
     5881 tcgaaggtgg aagcgatcag gtacgcggaa tccgacaagc tcggcccgct cggcacgaag
     5941 cgatcgatcc tcgaggacgg catcgagctg cgccgcatgc tcgtcgcaga attccagtcc
     6001 gcggccgacg ctgcgcgcga cgccgcgtcg cgcgtcgaca aagacgcagc tgccgccgtg
     6061 cacgactacc gcaaggcgct acgccgggcg cgtgcggtgc tcgcactcgc cgccggtgca
     6121 cttccgcgca gcgagcgtcg cgcgatcctc cgcgccctcc gcgaagcacg ccgcgcgctc
     6181 agcccggcgc gtgaccacgc ggtcgtgccc gacacggtcg cctcggtcga gctcgatgaa
     6241 gtcgaccgct cgacggccgc aacgatcatc tcctcgacag ccgactcggc gccgccgcaa
     6301 gccgagatca agcaactgct cgcggaaggt gccgcgcgcg ccgccgcgca agtcgaggcg
     6361 ctcgaagcgt cgtt
//
LOCUS       DASUFX010000573         7605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2037636, whole genome shotgun sequence.
ACCESSION   DASUFX010000573 DASUFX010000000
VERSION     DASUFX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037636"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFV99_23000"
     CDS             <1..720
                     /locus_tag="VFV99_23000"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5062265.1"
                     /translation="YVDERFPRQPRATSRQLIMTLLHIIDETLDEPDASLWEFRGIKR
                     RHCYTALCHWVGAKSAQKIARAAGDDAMLKIATRLATRAERLVEACYDSTRGAYMQEA
                     GSKYHDASSLQLITLHYLDPRSERAARHLESLERDLMTDGGLLHRYTHADDFGTPVSA
                     FLVCSFWHVEALAAMGRLEAALKQLELLRAYANHLGLFSEDIDPATGSQWGNFPQAYS
                     HVGMINAVLRISSRIDYPEFY"
     gene            complement(730..1383)
                     /locus_tag="VFV99_23005"
     CDS             complement(730..1383)
                     /locus_tag="VFV99_23005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5062266.1"
                     /translation="MTGGGSESERFSVILIDDHPLFRVGLAALIDAEPGLRVVGHAGT
                     SDEALALYDHVYVDLVVTVMQMGDGSGIDLVHSLAARRHVRILGLSGVVQPVKIAEML
                     RAGATSFALKSQSFPEVRDAIWETLEGRRYLAPSIKDEVEKVLNATEAQPHDRLTPRE
                     REISALVLRGYTNKEIGQSLFIAPRTVETHRLRMMRKLGIHTMSELIVMAARTGALD"
     gene            complement(1536..1919)
                     /locus_tag="VFV99_23010"
     CDS             complement(1536..1919)
                     /locus_tag="VFV99_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062267.1"
                     /translation="MTRLVVILTSTLLATACRDRTDSADRTGYSKPSETANDDFAHTR
                     SSYGKQMNERLQQLDARIHELAAKGTEKAKQAADELRVERDRLAPQVDEIGKQAREGW
                     DNFESELSRGFDNLEQKLNDALEHD"
     gene            1997..2992
                     /locus_tag="VFV99_23015"
     CDS             1997..2992
                     /locus_tag="VFV99_23015"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5062268.1"
                     /translation="MASPENPLVAVVTGASQGVGRAVARELGAHRAHVALVARNIDGL
                     AGAAEEINAAGGEALVLPLDVSDASAVDAAADAVVERWGKIDVWINNAMVSVFSPVAW
                     MTADEFRRVTEVTYLGYVYGTCAALRHMRKRDSGMIIQIGSALAYRSIPLQSAYCAAK
                     AAIRGFTDSLRCELLHDKSNIKITMLQLPAVNTPQFDVVRNKLGEHGQPVPPIFQPEL
                     IAHAVMHAIEHPTREMWIGWPTLKAIVAQRLAPGIADHYLASHGYASQVTTALPPTRS
                     DNLYAPLPGDRGAHGDFDHRAKDFSAELWLRLHRGAIAAAALGLLATALLARRWR"
     gene            2989..4800
                     /locus_tag="VFV99_23020"
     CDS             2989..4800
                     /locus_tag="VFV99_23020"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5062269.1"
                     /translation="MRINDYALIGDGRSAALVSRGGTIDWLCWPRFDSPPIFAKLLDP
                     AAGTWRIAPVGESKVTRRYIDDTNVLETRFETAAGTIIVVDLMAVANEEQKGLVPDHE
                     LLRHVCCVRGEVEIDFAIEPRPDFRAVTGVAHRTLGLRWQVGSRLLALRGDRPIDASG
                     CERFTMRAGEGLSFSLTFDEGPTALLPLGSHALDRIRRSIAWWHAWASRANYRGPHRD
                     RVIRSALAIKLMHFAPSGAVIAAPTTSLPEVLGGPDNWDYRFCWLRDASFTARAALSL
                     GYVEDAAAFCTWMLYTTRLTSPELDVIYDVYGNRPPKERALYHLEGFASSRPVRFGNA
                     AASQLQLDMYGEVIDATAQVAQVTGYLDRDTQRLLRMFGDYVCNHWQLPDQGIWEPRG
                     AAMRHTHSQLLCWVALDRLVELGRRGIVANLDLAKLATHRAAIRRDIETNAFDAELGA
                     YTGTLGGRELDASVLLMSWYGFHGPRSPRMSSTYARISERLGAGPGLLYRYDHSIPKR
                     EGAFWICSFWAVEHLALGGGTRAEARAMFDAACRYANDVGLMAEEVDPISCEPIGNFP
                     QAYTHVGLISAALSLEERARKSRGEQPMRNTELEVRA"
     gene            4803..5270
                     /locus_tag="VFV99_23025"
     CDS             4803..5270
                     /locus_tag="VFV99_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062270.1"
                     /translation="MGSGFVWGFAATIILTTMMSASQGLGLTRMNIPYLLGTMVTSDR
                     DRAKPIGVALHVINGWVFSFVYIAAFHVWGGPTWWKGAAIGLVHSAFVLAAAMPTFPG
                     FHPRMASETRGPTVVRVLEPPGFFGLNYGYRTPLSVVVAHVAFGVILGVFYRP"
     gene            5372..6943
                     /locus_tag="VFV99_23030"
     CDS             5372..6943
                     /locus_tag="VFV99_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062271.1"
                     /translation="MKPHARIRAVALGGTIAIVVASGCSNNNGNNDMEPDGGMGSNYG
                     GDGGGSDSSVPPMPTEFTVTLVPDGASGVQRVNFAVPLAQGALGDDAAIKVTAGSFDL
                     PAARKGLAKYADGSWRSVQIQVDVDVTANQSLTIALGTAGATGPSLDAVSTTLTGSGN
                     NVHPKVWALLPSEVLTASGVAGLLIPQADVANTALDAWSSICDYATWDTDKFLVNSAA
                     SRDVWLYDRVTAMYRGYAITGDLVPLRSAYREAEIYRAGMVISNGVATNIAVPDAQTD
                     LKYYYAQNMALHYLLTGDERYREAAEAVSAKIATMWNPNYDGGDRFWTERHAGFALLA
                     HEWALMVTDDQATTISARAEANVTAYLADQSTYPVGYSDPNARCFAHSATAHAEDYGY
                     NGCSPWMSAILADALDQYARRVGGTREAQVRDSLAKLGRILARDGRDATGKPFYWMGV
                     GTTSDEIDDYDEHWGESAYVVALAYAATGKTEPALKQAADQLIAGLAANGVVGQTRSF
                     NWQCRSAVMTPALLK"
     gene            complement(6947..7111)
                     /locus_tag="VFV99_23035"
     CDS             complement(6947..7111)
                     /locus_tag="VFV99_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062272.1"
                     /translation="MLRKHASLVIAVSLVALVTTACIVRTHTNRRAQPVYVEKHKDHG
                     KHKGHGKHKH"
     gene            7156..>7605
                     /locus_tag="VFV99_23040"
     CDS             7156..>7605
                     /locus_tag="VFV99_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062273.1"
                     /translation="MIEEATLRRRLFYGVAVVAGLGLLVDVWHARSPNDVVEALLPKL
                     SLSYEANLPTWVSSSLLLVCALVAGAIATARPPWHRHWWGITAILAWMSLDEAAEIHE
                     HLGGLIGTNGIFYFDWVIPATAVVLALALIYLPFVRVLESRTRNRLLI"
BASE COUNT         1283 a         2461 c         2450 g         1411 t
ORIGIN      
        1 tacgtcgacg agcggttccc tcgccagccg cgcgcgacgt cgcgccagct gatcatgacg
       61 ctgctccaca tcatcgacga aacgctcgac gagcccgacg cgagcctgtg ggagtttcgc
      121 ggcatcaaac gccgccactg ctacaccgcg ctgtgccact gggtcggcgc gaagtcggcg
      181 cagaagatcg cacgcgcggc cggtgacgac gcaatgctga agatcgccac gcgtctcgcc
      241 acgcgcgcgg aacggctcgt cgaggcctgc tacgacagca cgcgcggcgc gtacatgcag
      301 gaggccggca gcaagtacca cgatgcgagc tcgctgcagc tgatcacgct ccattacctc
      361 gatccgcgct ccgaacgcgc ggcgcgccac ctcgagtcgc tcgaacgcga cctgatgacc
      421 gatggcggcc tgctccaccg ctacacgcac gccgacgatt tcggtactcc ggtgtcggca
      481 ttcctcgtgt gctcgttctg gcacgtcgag gcgctggctg cgatgggcag actggaagca
      541 gcgctcaagc agctcgagtt gctgcgcgcc tacgccaatc acctcggact gttcagcgag
      601 gatatcgatc cggcgactgg aagccagtgg ggcaacttcc cgcaggcgta tagccatgtc
      661 ggcatgatca atgccgtcct gcgcatcagc agcagaatcg attatccgga gttctattga
      721 gcgagcggtt caatcgagcg cgcccgttcg cgcagccatc acgatcagct cggacatcgt
      781 gtggatgccg agcttacgca tcatgcgcag gcgatgggtt tcgaccgtac gcggcgcgat
      841 gaacaagctt tgtccgatct ccttgttcgt atagccacgc aacacgagcg cagagatctc
      901 gcgctcgcgc ggcgtcaacc gatcgtgcgg ctgcgcttcc gttgcgttca gcaccttctc
      961 gacctcgtct ttgatgctcg gcgcgagata gcggcgccct tcgagcgtct cccagatcgc
     1021 gtcacgcact tcggggaacg attgcgactt gagcgcgaag ctcgtcgcac ccgctcgtag
     1081 catctccgcg atctttaccg gctgcacgac acccgatagc ccgaggatcc gtacatgacg
     1141 tctcgccgcc aggctgtgca ccagatcgat gccgctcccg tcgcccatct gcatcaccgt
     1201 cacgacgaga tcgacataca catggtcgta gagcgcgagt gcttcgtcgc tggttcccgc
     1261 gtgcccgacg acacgaagac ctggttcggc gtcgatcaac gctgctagac cgacgcggaa
     1321 cagtggatgg tcatcgatca ggattaccga gaaccgttca ctctcactac cgccaccggt
     1381 catggaacgc ctgtagcaat tcacgagcgc cgtcgcaatc aaacagattc tggcgagatg
     1441 ctgtccctgc aacctgcagg ctaacaacta cgagggcagc ggacttcttt acgcgaactt
     1501 gattcggctg tgccgcttcg tctcactccg cacacctaat cgtgctcgag cgcgtcgttt
     1561 agtttttgct cgagattgtc gaatccgcga gacagctccg attcaaagtt gtcccagccc
     1621 tcgcgcgctt gtttgccgat ctcgtcgacc tgtggcgcga gtcgatcgcg ctcgacgcgc
     1681 agctcgtccg cggcttgctt cgctttctcg gtgcctttcg ccgcgagctc gtgaattcgg
     1741 gcgtcgagtt gctggagtcg ctcgttcatc tgctttccgt acgacgaccg cgtgtgcgcg
     1801 aagtcatcgt tcgcggtctc cgacggcttg ctgtagccgg tgcgatcggc gctatcggtg
     1861 cgatcgcggc acgctgttgc gagcagcgtc gacgtcagaa taaccacgag tctcgtcatc
     1921 gctcgtacgg tcatgcttct tctcggtgcg acgcacgtgc cagcaaacgg gacctcgctt
     1981 tgcaagattt ctcggcatgg cctctccaga aaacccgctg gtcgccgtgg tcaccggtgc
     2041 ttcgcaggga gtcgggcgcg ccgttgcgcg cgagcttgga gcgcaccgtg cgcacgttgc
     2101 actcgtggcg cgcaacatcg atgggctcgc gggtgctgcg gaagaaatca acgcagccgg
     2161 tggtgaggcg cttgtacttc cgctcgacgt ctcggatgcc tctgcagtcg atgcggccgc
     2221 cgacgcggtc gtcgagcgct ggggcaagat cgacgtctgg atcaacaacg cgatggtctc
     2281 ggtgttctca ccggtcgcgt ggatgaccgc ggacgagttc cgccgagtca ccgaggtcac
     2341 gtatctcgga tacgtctacg ggacgtgcgc cgcgcttcgt cacatgcgga agcgcgattc
     2401 cgggatgatc attcagatcg gctcggcgct cgcgtatcgc tcgattccgc tgcagagcgc
     2461 gtattgcgcc gcgaaggcgg caattcgcgg cttcacggac tcgctccgct gcgagctgct
     2521 tcacgacaag agcaacatca agatcacgat gctgcagctg ccggcggtca acacgccgca
     2581 gttcgatgtc gtgcgcaaca agcttggcga gcatggtcag ccggtgccgc cgatcttcca
     2641 gccggagctg atcgcacacg ccgtcatgca cgcgatcgag catccgacgc gcgagatgtg
     2701 gatcggttgg ccgacgctga aagcgatcgt cgcgcagcgc cttgcgcccg ggatcgccga
     2761 tcactacctc gcgtcgcacg gctacgcgtc gcaggtcact accgcgctgc cgccgacgcg
     2821 cagcgacaac ctctacgcac cattgcccgg cgatcgcggt gcacacggtg acttcgacca
     2881 tcgcgccaag gatttcagcg cggagctgtg gctgcggctt caccgcggtg cgatcgcggc
     2941 ggcggcactc ggtctgcttg ccacggcctt actggcgcgg cggtggcgat gaggatcaac
     3001 gactacgcat tgatcggcga cggccgctcg gcggcgctgg tatcacgtgg cggtacgatc
     3061 gactggctgt gctggccgcg cttcgacagt ccaccgatct ttgcgaagct gctggacccc
     3121 gctgccggca cctggcggat cgcaccggtc ggcgaatcca aagtcacgcg tcgttatatc
     3181 gacgacacga acgtgctcga gacgcgattc gaaaccgcgg ccggtacgat cattgtcgtc
     3241 gatctgatgg ccgtcgcgaa cgaggagcaa aaggggctcg ttccggatca cgagctgtta
     3301 cgccacgtct gctgcgtgcg cggcgaggtc gagatcgact tcgcgatcga gcctcgaccc
     3361 gactttcgcg cggtcaccgg ggtcgcgcac cgtacgctcg gtttgcgatg gcaggtcggc
     3421 tcgcgcctgc tcgcactgcg cggagaccgc ccgatcgacg cgagcggttg cgaacgattc
     3481 acgatgcgcg caggcgaagg cctgagcttc tcgctgacgt tcgacgaagg gccgacggcg
     3541 ctcctgccac tcggtagcca tgcactcgat cggatccggc ggtcgatcgc gtggtggcac
     3601 gcgtgggcca gccgcgcgaa ctatcgcggc ccgcatcgcg atcgcgtgat ccgcagtgcg
     3661 ctcgcgatca agctcatgca cttcgcaccg tcgggcgcgg tcatcgctgc gccgaccaca
     3721 tcgctgcccg aggtgctcgg cggccccgat aactgggact atcggttctg ctggctgcgc
     3781 gacgcgtcgt tcactgctcg tgccgcgctc agcctcggtt acgtcgagga cgcggccgcg
     3841 ttctgtacgt ggatgcttta cacgacgcgg ctgaccagtc ccgagctcga cgtgatctac
     3901 gacgtctatg gcaatcggcc gccgaaggag cgcgcgcttt accacctcga gggctttgcg
     3961 agctcacggc ccgtgcgctt cggcaacgcg gccgcgtcgc agctgcagct cgacatgtac
     4021 ggcgaagtca tcgatgcgac cgcgcaggtc gcgcaagtca ccggctatct cgatcgcgat
     4081 acccagcgct tgttgcgcat gttcggcgac tacgtctgca atcactggca gctgccggat
     4141 caaggcatct gggagccacg cggcgcggct atgcgtcaca cgcactcgca gttgttgtgc
     4201 tgggtagcac tcgatcgatt ggtcgagctc ggtcggcgag gcatcgtcgc gaacctcgat
     4261 ctggcgaagc tcgcaacgca ccgcgcggcg attcgccgcg acatcgagac gaacgcattc
     4321 gatgccgagc tcggtgcgta cacgggcacg ctcggcggcc gcgagctcga cgcgagcgtg
     4381 ctgttgatgt cgtggtacgg ctttcacggt ccacggtcgc cgcgcatgag ctcgacgtac
     4441 gcgcggatca gcgagcggct cggcgctggg ccaggccttc tctatcgcta cgaccacagt
     4501 atcccgaagc gcgagggcgc gttctggatc tgtagcttct gggccgtcga gcacctcgcg
     4561 ctgggaggcg gcacccgtgc cgaagcaagg gcaatgttcg atgcggcgtg tcgttacgcg
     4621 aatgacgtcg gcctgatggc cgaagaggtc gatccgatct cgtgcgagcc gatcggcaac
     4681 ttcccgcagg cgtacacgca cgtcggtctg atcagcgccg cactatcact cgaggagcgc
     4741 gcacgcaaat cgcgtggcga gcagccaatg cgcaacaccg agctcgaggt acgcgcatga
     4801 atatgggcag cgggtttgtg tggggcttcg cggccaccat catcctgaca acgatgatgt
     4861 cggcgagtca gggcctcggc ctgacgcgga tgaacattcc gtacctgctc ggcacgatgg
     4921 tcacgtccga tcgcgatcgc gcgaaaccaa ttggcgtcgc gcttcacgtc atcaacggtt
     4981 gggtgttttc gttcgtgtac atcgcggcat tccacgtgtg gggcggcccg acgtggtgga
     5041 agggcgcggc gatcggtctc gtccactcgg cgttcgtgct cgccgccgcg atgccgacgt
     5101 ttcccggctt ccatccccgc atggcgagcg agacgcgtgg gccgaccgta gtgcgcgtgc
     5161 tcgagccgcc cgggttcttc ggactgaatt acggttaccg aacgccgctc tctgtcgtgg
     5221 tggcgcatgt cgctttcggc gtgattctcg gcgtgttcta tcgtccgtaa gtcttcgcag
     5281 ccaccagcac gcgcaagcgt gattacggcc gagcgatcaa caaacgattt cgttggcgac
     5341 ggacaccggt gcgcatctag catcgccgcc gatgaaaccc cacgcgcgca ttcgcgctgt
     5401 ggctctaggg ggaacgatcg cgatcgtcgt cgctagcggc tgcagcaaca acaacggcaa
     5461 taacgacatg gagcccgacg gtggaatggg ctccaactac ggtggtgacg gcggtggcag
     5521 cgacagctcg gtcccgccga tgccgaccga gttcacggtc acgctcgtgc ccgacggggc
     5581 gagtggcgtg cagcgcgtga acttcgccgt gccgctggcg caaggtgcgc tcggtgacga
     5641 cgcagcgatc aaggtgaccg ccggttcgtt cgacctcccg gccgcgcgca aaggcctcgc
     5701 gaagtacgcc gatggcagct ggcgcagcgt gcagatccag gtcgatgtcg acgtcaccgc
     5761 gaatcagtcc ctgacgatcg cgctcggcac cgctggtgca accgggccat cgctcgacgc
     5821 cgtgagcacg acgctgaccg gtagcggcaa caacgtgcat ccgaaggtgt gggcactgct
     5881 gccgagcgag gtgttgaccg cgagtggcgt ggctgggctc ctgatcccac aggctgatgt
     5941 tgcaaacacc gcgctcgatg cgtggtcgtc gatctgcgac tacgccacgt gggacacgga
     6001 caagttcctc gtcaattcag ccgcgagccg cgacgtgtgg ctctacgatc gagtgaccgc
     6061 gatgtatcgc ggctacgcga tcaccggcga tctcgtgccg cttcgctcgg cgtatcgcga
     6121 ggcggagatc tatcgcgccg gcatggtgat ctcgaatggc gtcgcgacga acatcgccgt
     6181 gcccgacgcg cagaccgacc tcaagtacta ctacgcgcag aacatggcgt tgcattactt
     6241 gctcaccggc gacgaacgct atcgcgaggc cgccgaagcc gtgtctgcca agatcgcgac
     6301 gatgtggaac ccgaactatg acggcggcga tcggttctgg accgagcgtc acgcgggatt
     6361 cgcgctgctc gcgcacgagt gggcgttgat ggtcaccgac gatcaggcca ccacgatcag
     6421 cgcgcgcgcc gaggcgaatg tcaccgcgta cctcgccgat cagagcacct atcccgtggg
     6481 ctacagcgat ccgaacgcac ggtgtttcgc gcacagcgca accgcgcacg ccgaggacta
     6541 cggctacaac ggttgcagcc cctggatgag tgcgatcctc gccgatgcac tcgatcagta
     6601 cgcgcggcgc gtcggcggca cgcgggaggc gcaagtgcgc gattcgctcg cgaagctcgg
     6661 ccgcattctc gcgcgcgatg gccgcgacgc gacgggtaag ccgttctact ggatgggcgt
     6721 tggcacgacg agcgacgaga tcgacgacta cgacgagcac tggggcgagt cggcctatgt
     6781 ggtcgcgctc gcgtacgcgg cgaccggcaa gaccgagccc gcgctgaagc aggctgccga
     6841 tcaattgatc gcgggtctcg ctgccaatgg cgtcgtcggt cagacgcgca gcttcaactg
     6901 gcagtgccgt agtgcggtga tgacaccggc gctgctcaag tagcgctcag tgtttgtgct
     6961 tgccgtggcc tttgtgcttg ccgtggtctt tgtgcttctc gacatagacc ggctgcgcac
     7021 gacggttggt gtgtgtgcgc acgatgcagg ccgtggtcac cagcgcgacc agggaaactg
     7081 caatgacgag cgaggcatgc ttccgaagca tcagctacaa cgatatcgga cggcgcgctc
     7141 cgatacaatg acgccgtgat cgaggaggcc acgctgcgcc ggcgcctgtt ctatggcgtc
     7201 gctgtcgtgg cgggcctcgg tctcctcgtc gacgtttggc atgcgcgctc accgaacgat
     7261 gttgtcgagg cgctgctgcc aaagctgtcg ctgagctacg aggcaaatct gccgacgtgg
     7321 gtgtcgtcga gcctgctact cgtgtgtgcg cttgtcgctg gggcgatcgc cacagcgcgg
     7381 ccgccctggc accgccattg gtggggcatc accgcgatcc tcgcgtggat gtcgctcgac
     7441 gaagcggccg agatccacga gcacctcggc gggttgatcg gcacgaacgg catcttctat
     7501 ttcgactggg tgattccggc gacggccgtc gtgctcgcgc tcgcgctgat ctatctgccg
     7561 ttcgtccgcg tgctcgaatc gcgcacgcgc aatcgcttgc tgatc
//
LOCUS       DASUFX010000574        17919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1204034, whole genome shotgun sequence.
ACCESSION   DASUFX010000574 DASUFX010000000
VERSION     DASUFX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204034"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3935)
                     /locus_tag="VFV99_23045"
     CDS             complement(<1..3935)
                     /locus_tag="VFV99_23045"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEX5062274.1"
                     /translation="MSPRYWIAAVAVIAAMGTSPPVRAEPKNEVRSVSFDEDAGITRV
                     HVRGATTPTFTVYKLERPTRVVIDLPQARLSEAMKGHESAAVFTPNTWAVSTIAAQQL
                     DDGAVRVIVSLARPGRYDVKTDGNEVVVMVMPRDPAPKTANPADLARMQAENEQAKKQ
                     AQAAEQARVAAEKSSEAAGATAKQSRAEADAAKAEAERLRKLAADQAAKAEAAQRAVD
                     DAKKQLAKNAASSADFARAQQIAQAAKDEAARAKAQAAKATGDAEAAKQSAQKAQVEA
                     ERMKVMAEQARRDALAEAAAAKADAAKARTDMARTQADAAKAREEIVRTQADAAKARD
                     EVARSQAEAAKVRTDMARTQADAAKARAEIMRTQADAAKARDEVARSQAEAAKARDDM
                     ARTQAEAQKAREEMARTQAETQKAREEAARRQVEVTKAREEVARSQAEAQKVREEIAR
                     KQLEATKAREDMARTKAEADKIRAEIAQKQQEATKARNDMTRTQQEADRAKAEVARAQ
                     ADAQAAVAKSRAEADQAKRDAAVAITAAQHEAQVAKSDAQSAKADAARARSEAETAKA
                     QAAADKKQAEADKQRAEVARKDAEKMLKDAKSQLATLEQKTLSAQQMDDKARASLAAA
                     QAREEAARDTVARAVKEREVAEAAAKRAQDARDRAAVTDRSTADANAKAAEERLAKAR
                     QAAGEADARRLAAEQLATQAKMELAQTKTALATVEQQRADAVAAATDAARKRSEAEAA
                     ASDAARRRGEAEVAATEAAKKRREAETAATEAAKRRSQAEIDRAHAESQRVAAEQAAR
                     EAQSAKDAAEKQRKVAEASAQQALAAKRDAEASIATLTAKRQAAEKAADEVAARAKAE
                     AKAQADVAAAQARKADADEIAAARREVARLGDERKKAEAELAERRKAAADQQAESTRL
                     ATAAAQAKDAADREETRRTQLAVQRAQEEQELARIKAQKVQAQVAQAQAAQAQAAQAQ
                     AAPTPTRTAAPVKTAPVKTAPLKIAPVKIAPVKIAKVNNVTFKGNADSGVVELAMAGD
                     GDVQLGEITASHIELIVDHAELPVKLERTLDVSKFGSAVKKVSSFRDRRTPNRVRFVA
                     ELASPAIPTVVRDGNTVRWRFASNDVAQAKPMRTQSVPVPVVGGFGAASTPVAQQSVS
                     QVPPAAGGRGRKIYHGATVEFHFKDAPIHDLLRIIADVGGVNIVVPDTIDAKVTVDMK
                     RVPWDQALDVILASHGLWYRREGNLYRIATRKEIDAEDEAEAARRAAAANSEMPRPEV
                     VTLNYASADDLRPKLESMLSPKGKIEVDGRTNALIVSDIAAVRSEIAKLA"
     gene            complement(4084..4863)
                     /locus_tag="VFV99_23050"
     CDS             complement(4084..4863)
                     /locus_tag="VFV99_23050"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX5062275.1"
                     /translation="MRLPPLLAIALLAAACGEDAPPAPPKKAGAPAPGRPGAPGQKAK
                     QLQARTHVEERVTCPEIEKATGPKCDPAAASCEAGLYCVPVKTGGNFCEPCPERDSIR
                     HDFKDRDFVADQVRDPFQPFIIPLKGLEPQSDKKVEGPCNRPEQFVAGNYSYLDLRLV
                     GIVAQGTQRKVLMMDRGNVGHIIRRGDCVGKEKAVVKDIGPGFVTFVIHPDADDKTPN
                     RVPEERSVLLNPKGLQVAPEAPPEASGSQPSAPIVAPPANQ"
     gene            complement(4863..5684)
                     /gene="pilO"
                     /locus_tag="VFV99_23055"
     CDS             complement(4863..5684)
                     /gene="pilO"
                     /locus_tag="VFV99_23055"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX5062276.1"
                     /translation="MAVAADFARMPTRQKVMVFIVIGALLGAVYWQFVYKGLEQKLEA
                     AKGENASKKALNKKLEAQIPEFETLKTRMISLKRMIEENQKALPTSAELPAFFETLNR
                     KVLESGVEVIRSKQGKEEAVETFVKVPIDYEIQGTYTQIKKFFASLVPKKKKPGQQPE
                     PQPGGDQTVEERERIVSIENLSLSAPTVRNREIRLTARFTASTYRQDEKPGAAPAPKA
                     AAPAAPPATAPASTPKGAKQQTENAIDKGDKKDRDAAGVNEAKTPSGVDRMKEGK"
     gene            complement(5684..6355)
                     /locus_tag="VFV99_23060"
     CDS             complement(5684..6355)
                     /locus_tag="VFV99_23060"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5062277.1"
                     /translation="MIKINLLPQKRAKMRGAAAAAREPGTKDIVFGVVGLAVAAVAVF
                     AIVDAPRRSRLTALNETNAQLQQEISAKQKQLQGFDDLKKSEQEANERAQAINRLNSA
                     KVVPANVLHELSQILSPVGPTMTEDMAKKTGTGIDSDPNKRFDLAWDPTHVWMTVFND
                     NAKDGTFRLEGGAQAQIDIIQLSKRLQASVYFDNVSQQSEERQTDRESGITFYKFVIT
                     GKVAY"
     gene            complement(6360..7406)
                     /gene="pilM"
                     /locus_tag="VFV99_23065"
     CDS             complement(6360..7406)
                     /gene="pilM"
                     /locus_tag="VFV99_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX5062278.1"
                     /translation="MAKQCIGLDIGSSAVKAVQLKRKGSSWALQAFGMQPLVPQTIVD
                     GTIMDQGAVTDAIRQLWSRLRLKTKDVAIAIAGHSVIIKKITVPMMRPEELAANIRNE
                     AEHHIPFGRDDVEIDYHVTNPMTASGQTELLLVAAKKEVVSDYVQVVRDAGLSPQVVD
                     VAAFASQNGFEANYPLDPRETVVLINIGAAISNINIVRSGVSLFTRDVTIGGNAYTEE
                     IQKQMGIAADEAEAYKVGGTQTEDGVVPQEVIRVMEGVSEVMAGEFQRSLDFFLATTA
                     DANVTRIVLAGGSSKVASLHRAIERRSRLPLEVADAWKRVEIDPSLDRTYLAAHSPEA
                     LIGVGLSMRASGDK"
     gene            complement(7487..8845)
                     /locus_tag="VFV99_23070"
     CDS             complement(7487..8845)
                     /locus_tag="VFV99_23070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5062279.1"
                     /translation="MTSAPAPGAVHRVLVLGPDDEARRALHMLVAKHGWNVAAAAELD
                     AAKKHFAASDCDVVIAVPDLAPQVCALADAPPVIAVVRTRDVALATSLLEAGVDDILT
                     EPIDELALALALRRPTWRAKDANGNSNGTSNGKGFLAAPDIIGDGPVMQKLKSTIKVV
                     APTRTTVLIQGESGTGKELVARAIHDASPRRHKRFVAINCAAIPATLLESELFGHVRG
                     AFTDAVRDKVGLFEEADGGTLFLDEVGELPLPLQAKLLRALQESEIRRVGDSAPIKVD
                     VRLIAATLRDLAGEVAAGRFREDLFYRLNVLPVAVPALRDRTEDIPQLVRFFVARHAA
                     RHNRDVEVSDGAIEALAKQPWPGNVRELENVIERALVLSDGPAIDLAFLTTVMHVRAG
                     GSASQADDHELSIKKATRSLEQDLIRRALEVTQGNRTNAAKLLEISHRALLYKMKEYG
                     IS"
     gene            8964..10046
                     /locus_tag="VFV99_23075"
     CDS             8964..10046
                     /locus_tag="VFV99_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062280.1"
                     /translation="MRPTIMMRVVPAVAVTLGIAACVEPRRPGEPGDDASRVTLLELT
                     ETPRTKSDLLIVIDDTTAMAPVRSQLATLPARLADRLDWYTRRWIDLRVAVATNDGTL
                     RRLPGVDTPWVADAFDFDYTRRRNYDGTLADALTALMDVGAANPSTSQPLEAARHALE
                     TGTELMRDNAGLMVLTISAADDASSLPVSDYVNWMWSVSNGQTWWRDVLLSGLYVQPA
                     SRLDEYYKAFPWSIITPLDSGNVEAAIPWVPEGSGNGGQSADSWVGLGCYELADLDAA
                     IPGLDHECQMMVSINNEWRELPECTASQRIERTERERGLSGPPEACWWLRPNFESCLY
                     TTGREIALSGYTGAQHPALRFDCRAR"
     gene            10187..11245
                     /locus_tag="VFV99_23080"
     CDS             10187..11245
                     /locus_tag="VFV99_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062281.1"
                     /translation="MTRLLPAIAVTLGTVACVDTPPFAEPEVDQSRVEVRELPASPLS
                     KLDVLIVVDDSIAMAPYRDRVQALSSMLTDTLANFAEGWANVRIAVTTNDGKLRRVPT
                     TNAPYAIDAREYDFTHRANYIGRLAETVAAMVDVGVDGTGPSQPLAAMVHALETNSQF
                     LRDDSAFAVLMIGASDDASPFTVDEYVNWMKRLTGGFWRRRVVVVGLYPESAPRIAEY
                     FRGIHEVGFAKPIDYDGDLRLALVNLLVFDRWTDLVGSPCLESTPLLLDPSAADDRYD
                     CTMSVDLERGLVAVPQCDRARPDGERTDGFAPRATSACWFFRADPQNCTLENHLSLGL
                     SGYTLLHHPALRLECRTR"
     gene            11242..12294
                     /locus_tag="VFV99_23085"
     CDS             11242..12294
                     /locus_tag="VFV99_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062282.1"
                     /translation="MMKCALTALVLAAACVDTPPLQEQHVESSRVTIREIPETPRATT
                     DLLIVVDDTVGMAPYQAQLATLPARLASRIDWYTRRWIDLRLAVATNDGTLRRLPGMD
                     TPWLADAFDFDYTRRRNFDGTLADRLAELMAVGTANEGPSQPLEAGRRALETGSELMR
                     DKTGLMVLTISATDDASTWPVSDYVEWMTKLAGGDFWGRGVLLSGLYLQPAARLDEYF
                     KATHAIITPLDGDNFEAAIPWFPAGPNTVTFGCSEAVDLDPTTPAFDYDCAMMASING
                     TWRTVPECTPNQRDGGEVHDRNIRERGLAGPPESCWAFRPDPQSCFYGDSKSQLVMSG
                     YTAFQHPALRFDCRSN"
     gene            12294..13340
                     /locus_tag="VFV99_23090"
     CDS             12294..13340
                     /locus_tag="VFV99_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062283.1"
                     /translation="MKRTLLVLALSAACTGDDSGGGVQGTAHVETYELPAALPPKLDI
                     LFVIDDSTAMASHQTPLAAMPAQLEQLVSGTYGDAVNYHIGVVTTDAASGGNLRTAAG
                     INGTFIVHENTFTGATTNYQGSFATALGSLFPSSATSTASNQPLATMRAALENTVANA
                     GFLRSEAYLGVVTISATDDTSGGGAAADYAAFLKSSKTDPTSVIVSGVIDASATRLNA
                     FHDEFPNRTAKTSIAGTDYGSALELFGQLYRTVLGYACNKEPADVDPTTPGPQYDCAF
                     VWVDQIDGHTEHRLPPCVGGDTSGCWEIVVADPSICTDPSTKAHLQTRGFTTSPSKYG
                     DPFHPTIRGQCLVN"
     gene            13598..14947
                     /locus_tag="VFV99_23095"
     CDS             13598..14947
                     /locus_tag="VFV99_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062284.1"
                     /translation="MPQQGRVEYKDIPVTVNRDIDILFIIDDSPSMLDKQTNLKNNFP
                     NFINVLNTIEGGLPNVHLGVASSDLGTKGAADAQAAPGIGSGPGSCSGNGKSGNLQLG
                     NPASTLVTGNFISDTKNPDGTRTTNYSGSLANAFSSIASLGASGCGFEQHIEAAKRAL
                     NNNPANAGFMRPNAYLALIFIQDEDDCSMNHATLVGSDTTTLGPLQSFRCNRFGHKCT
                     TGGNNSTEMNAVGPKSGCASEESGTYLTKIGDYVTFFKGLKADPNNVIVAAISGPTTP
                     YEVELRTPPGGGTAIPAVAHSCTYQGMNGPEVADPSVRLKQFLDSFPNRSTFSTICQQ
                     DLSGALIQIAQLLRTVIGSPCIEGKLADVDPTTPGPQYDCSVSDVRNYGKANQSEEVL
                     PQCNNLGTPASSTNKPCWAIETDTMNCTAGDHLTLKVERNEAPAADTHVISYCVTEA"
     gene            15079..16431
                     /locus_tag="VFV99_23100"
     CDS             15079..16431
                     /locus_tag="VFV99_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5062285.1"
                     /translation="MRIREVLFALVMAGCADRTIASVNPVQGKVETLDLPSSLEKDVD
                     ILFLIDNSGSMLAEQQSLQANFPKFMQVLETLQGGAPNMHIGVATSDMGQHATDGNGT
                     NSAAGGCTGAGDDGVLRTTPAVSGRFIIDEESGGTRNRNYSGTLGDAFAAMAGVGTDG
                     CGIEQHLGAVERALGNTANAGFVRPNAKLAVIVIADEDDCSLAHNSLFNGTTAQLNFR
                     CTQTGITCTDNPDLSKPGVRTMCQPNDQSQYLEPVDKYVDFIKTLKPNWRDDVIVAGI
                     VGDDNPFEIKLDAMNNPILGPSCTYNGGDGAVPALRTKDFLEQFTYTVKRSICGADLS
                     QAMVDIAALLKRSIGDPCWEGEIADLDPAPGLQADCSVVDVRVLPDGTRQEVAQIPSC
                     SVAGGQIPCWHIEEDAMECFYTSTHLKIVVDRGGVIPPADMRLNASCVTTDDGSGGFM
                     "
     gene            16505..17569
                     /locus_tag="VFV99_23105"
     CDS             16505..17569
                     /locus_tag="VFV99_23105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5062286.1"
                     /translation="MPAQGRPLITANQVTLARLIPMPLLAWLIYEGNRVHNNSYLWGA
                     LIAGTLIGCTDWVDGMLARKYGPTVLGGLLDPIADKVFIAFAYTPFADSGMVPWWACA
                     LMFTREFFITALRSAYEQRGLKFKTSYLAKAKTWTQMQGIGILLLFPLLDNKAILLWL
                     FAAGATIPIVVLVVWKIVKGEWWRGAFVMTGSFGALLAIFLIDESYAPRALMIGIVGI
                     TWLSGLDYIVGGWPKLRAYGDFSRADFVRLLGSLTLPILLFAVLVYTDAPRWTIFAIL
                     AFELAVGGLDNLLSHHKAATLALAWGGRVLGVSLLLGGALLMKDQAKALAIVACAVSV
                     FGCTAEFIRGRAYYIDHLME"
     gene            17589..>17919
                     /locus_tag="VFV99_23110"
     CDS             17589..>17919
                     /locus_tag="VFV99_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062287.1"
                     /translation="MRHAWWLVLAACGSSGSSPDASSDAVCEMDCAGVCNGSAMTDRC
                     GVCDTDPTNDCWKGVHGLSSLTPLPDQANLGSGKLAYEVSSVAQQRGLFYGGPAMTMP
                     ALGIAQVT"
BASE COUNT         2837 a         6124 c         5554 g         3404 t
ORIGIN      
        1 gcgagcttcg cgatttcgga gcgcaccgca gcgatgtcac tgacgatcag cgcgttcgtg
       61 cggccgtcga cctcgatctt gcccttcggc gacagcatgc tctcgagctt cggccgcaga
      121 tcgtcggccg acgcgtagtt cagcgtgacg acctcgggcc tcggcatctc ggagtttgcc
      181 gccgcggcgc gacgcgctgc ctcggcctcg tcctcggcat cgatctcctt gcgggtcgcg
      241 atgcgataca ggttgccttc gcgccggtac cacaggccgt gcgacgcgag gatcacgtcg
      301 agtgcctgat cccacggcac gcgtttcatg tcgacggtga ccttcgcgtc gatcgtgtcg
      361 ggcacgacga tgttcacgcc gccgacgtcc gcgatgatgc gaagcaggtc gtggatcggc
      421 gcgtccttga agtgaaactc gacggtggca ccgtgataga tcttgcggcc gcgaccgcct
      481 gccgccggcg gcacttgcga taccgactgc tgcgcgactg gcgtagatgc ggcaccgaag
      541 ccaccgacga ccggcaccgg cacgctctgc gtacgcatcg gcttggcctg ggcgacgtcg
      601 ttcgacgcga accgccagcg cacggtgttg ccgtcgcgca cgaccgtcgg gatcgccggc
      661 gacgccagct ccgcgacgaa gcgcacgcga ttcggcgtgc ggcgatcacg gaacgacgac
      721 accttcttca ccgcgctacc gaacttgctg acatcgagtg tgcgctcgag cttcaccggc
      781 agctccgcgt ggtcgacgat caattcgatg tgcgacgcgg tgatctcgcc gagctgcaca
      841 tcaccgtcgc ccgccatcgc gagctcgacg acgccgctgt cggcgttgcc cttgaacgtg
      901 acgttgttga ccttcgcgat cttcacgggc gcgatcttca cgggcgcgat cttcaggggc
      961 gcggtcttca ccggcgcggt cttcaccggc gcggcggtgc gcgtcggcgt cggcgcagcc
     1021 tgagcttgcg cagcctgagc ttgcgcagcc tgagcttgcg cgacctgcgc ctgaaccttc
     1081 tgcgccttga tgcgcgcgag ctcttgttcc tcttgtgcac gctgaaccgc gagctgcgtg
     1141 cggcgcgtct cttcgcgatc cgccgcgtcc ttcgcctgcg ccgctgcggt cgcgagccgc
     1201 gtcgactccg cctgctgatc ggcagccgcc ttgcggcgtt cggcgagctc ggcttcggcc
     1261 ttcttgcgct cgtcaccgag acgcgcgact tcacgccgcg ctgcggcgat ctcgtctgca
     1321 tccgccttgc gcgcctgtgc cgccgcaaca tcggcttgcg ccttcgcttc tgccttcgcg
     1381 cgcgcagcga cctcgtcggc ggccttctcg gctgcctgac gcttcgcggt cagtgtcgcg
     1441 atgctcgctt cggcgtcacg ctttgcagcg agtgcctgct gcgccgacgc ctcggccacc
     1501 ttgcgctgct tctcggccgc gtccttcgca gactgcgcct cgcgcgctgc ttgctcggcc
     1561 gccacgcgct gcgattcggc atgcgcgcga tcgatctcgg cctggctgcg gcgcttcgca
     1621 gcctcggtcg ctgccgtctc cgcctcacga cgcttcttcg ccgcctcggt ggccgccacc
     1681 tcggcctcgc cacggcgccg cgctgcatcg cttgccgctg cttccgcttc gctacgcttg
     1741 cgtgccgcat ccgtcgccgc tgcgactgcg tccgcacgct gctgctcgac ggtcgcgagc
     1801 gcggtcttcg tctgcgcgag ctccatcttc gcctgcgtcg cgagctgctc ggccgcgagt
     1861 ctgcgtgcgt cagcttcgcc ggctgcctgg cgcgccttcg cgagtcgctc ctcggcagcc
     1921 ttcgcattgg cgtcggccgt gctgcgatcc gtgaccgcgg cgcgatcgcg cgcatcctgt
     1981 gcacgcttcg ccgccgcttc cgcaacttcg cgctccttca cggcgcgcgc gacggtgtcg
     2041 cgagcggctt cttcacgcgc ctgtgctgct gcgagcgaag cacgcgcctt atcgtccatc
     2101 tgctgcgccg agagcgtctt ctgctcgagc gttgcgagct ggctcttcgc atccttcagc
     2161 atcttctcgg cgtccttgcg cgcgacctcc gcacgctgct tgtccgcctc ggcttgcttc
     2221 ttgtcggctg ccgcctgcgc ctttgcagtt tcggcctcgc tgcgtgcacg agcggcatcg
     2281 gccttcgccg actgcgcgtc ggacttcgcg acctgtgctt cgtgctgcgc ggcggtgatc
     2341 gccaccgctg cgtcgcgctt cgcctgatcc gcttcggcgc gcgacttcgc gactgccgcc
     2401 tgcgcgtcgg cctgcgcacg cgcaacctct gccttcgcgc ggtcggcctc ttgctgcgtg
     2461 cgcgtcatgt cattgcgcgc cttcgtcgct tcttgctgct tctgcgcgat ctcggcgcga
     2521 atcttgtcgg cctccgcctt ggtacgcgcc atgtcctcgc gtgccttcgt cgcttcgagc
     2581 tgcttgcgcg cgatctcctc gcgcaccttc tgagcttcag cctgcgaacg cgcgacttcc
     2641 tcgcgcgcct tcgtcacttc gacctggcga cgtgcggctt cttcgcgagc cttctgcgtc
     2701 tcggcctgcg tgcgcgccat ctcctcgcgc gccttctgag cttcggcttg cgtacgcgcc
     2761 atgtcgtcgc gtgccttcgc tgcttccgcc tgactacgcg ccacttcatc ccgcgccttc
     2821 gccgcgtcgg cctgtgtgcg catgatctct gcgcgcgcct tcgctgcatc tgcctgcgtg
     2881 cgcgccatgt cggtgcgcac cttcgctgct tccgcctggc tgcgcgccac ttcgtcgcgt
     2941 gccttcgctg cgtcggcctg cgtgcgcacg atctcttcgc gtgctttcgc tgcgtcggcc
     3001 tgcgtgcgcg ccatatccgt gcgcgccttc gctgcatctg ccttcgccgc ggccgcttcc
     3061 gcaagcgcat cgcgccgcgc ctgctcggcc atgaccttca tccgctctgc ttcgacctgc
     3121 gccttctgcg ccgactgctt cgccgcttcg gcatcgccgg ttgccttcgc cgcctgtgcc
     3181 ttcgcgcgcg cggcttcgtc cttcgctgcc tgcgcgattt gttgcgcacg cgcgaaatcc
     3241 gccgagctcg ccgcattctt cgcgagctgc ttcttcgcat cgtcaaccgc acgctgtgcc
     3301 gcttctgcct ttgcagcttg atccgcggcg agcttgcgca gccgctccgc ttccgccttc
     3361 gccgcatccg cctccgcacg gctctgcttc gccgtcgccc ccgctgcttc gctggacttc
     3421 tcggctgcaa cgcgcgcctg ctcggccgct tgcgcctgct tcttcgcttg ttcgttctcg
     3481 gcctgcatgc gcgcgaggtc cgccgggttc gccgtcttcg gcgccggatc gcgcggcatc
     3541 accatcacga cgacttcatt gccatccgtc ttcacgtcgt agcgaccggg ccgcgcgagc
     3601 gacacgatca cgcgtaccgc gccgtcgtcg agctgctgcg cagcgatcgt cgagacggcc
     3661 cacgtattcg gcgtgaacac tgccgcgctc tcgtgcccct tcatcgcctc cgacaaccgc
     3721 gcttgcggca gatcgatcac gacacgcgtc ggtcgttcga gcttgtacac ggtaaacgtc
     3781 ggcgttgttg caccgcgtac gtggacacgc gtgatgcctg cgtcctcgtc gaacgagacc
     3841 gaacgcactt cgttcttcgg ctcagcacgg acgggcggtg aggttcccat cgcggcgatc
     3901 actgcgactg cggcgatcca gtatctgggg ctcatgatca ttgcgactcc tgcgaaacgc
     3961 ggacaaacga cgggtgtggc acctcgaaag gtgcaggttc cccgcggtca gccgcgcggc
     4021 aaaaaaaatt gtcgcccggc gagcgcgcag atcacaaatt ttcgatcact gcgcgcgagt
     4081 cacttattga tttgcgggcg gcgcgacgat cggagcgctc ggctgcgagc cactcgcctc
     4141 gggcggcgct tccggcgcga cctgcagacc cttcgggttc aggagcaccg atcgctcctc
     4201 ggggacgcgg ttcggcgtct tgtcgtcagc gtccgggtga atgacgaacg tgacgaagcc
     4261 cgggccgatg tctttgacga cggccttctc tttaccgacg caatcaccgc gccgaatgat
     4321 gtgaccgacg ttgccgcgat ccatcatcag caccttgcgc tgtgtgcctt gcgcgacgat
     4381 tccgacgagg cgcaggtcga gatacgagta gttgccggcg acgaactgct cgggccgatt
     4441 acacggaccc tcgaccttct tgtcgctctg cggctcgaga cccttcagcg ggatgatgaa
     4501 cggctggaac gggtcacgaa cctggtcggc gacaaagtcg cggtccttga agtcgtgccg
     4561 gatgctgtcc cgctccgggc acggctcgca gaaattgccg ccggtcttca cgggcacgca
     4621 gtagagaccg gcctcgcacg atgccgctgc cggatcgcac ttcggtcccg ttgccttttc
     4681 gatctcgggg cacgtcacgc gttcttcgac gtgagtgcgc gcctgcagct gcttcgcctt
     4741 ctgcccagga gcaccgggac ggcccggcgc aggtgcacca gccttcttcg gcggtgccgg
     4801 cggcgcgtct tcaccacacg cggcggcgag cagcgcgatt gcgaggaggg gcggaagacg
     4861 catcacttgc cctccttcat gcgatcgaca cccgacggcg tcttcgcttc attgacgcca
     4921 gcggcatcgc gatccttctt gtcacccttg tcgatggcgt tctcggtctg ctgcttcgcg
     4981 cccttcggcg tcgacgcagg cgccgtggcc ggcggtgccg cgggtgcagc ggccttcgga
     5041 gcaggcgctg cgcccggctt ttcgtcctgg cgataggtcg acgcggtgaa ccgagcggtc
     5101 aaccggatct cgcgattgcg cacggtcggt gccgagagcg acaagttctc gatcgacacg
     5161 atgcgctcgc gctcttcgac cgtctgatcg ccccccggct gcggctcggg ctgctgaccc
     5221 ggtttcttct tcttcggcac gagcgacgcg aagaacttct tgatctgcgt gtacgtgcct
     5281 tggatctcgt aatcgatcgg caccttgacg aatgtctcga ccgcttcctc tttaccctgt
     5341 ttgctgcgaa tcacctcgac gcccgattcg agcaccttgc ggttcagcgt ctcgaagaat
     5401 gcaggcagct ctgccgaggt cggcagcgcc ttctggttct cctcgatcat ccgcttgagc
     5461 gagatcatgc gcgtcttgag cgtttcgaac tcggggattt gagcctcgag cttcttgttg
     5521 agtgctttct tgctcgcgtt ttcgcccttc gccgcctcga gcttctgttc gagccctttg
     5581 tagacgaact gccagtacac cgcaccgagc agagcgccga tcacgatgaa caccatgacc
     5641 ttctggcgcg tcggcatgcg cgcaaagtca gctgcgaccg ccattagtag gccacctttc
     5701 cggtgatcac gaacttgtag aacgtgatgc ccgattcgcg gtcggtctga cgctcctccg
     5761 actgttgcga gacgttgtcg aagtacaccg atgcctgcag gcgtttagac agctggatga
     5821 tgtcgatctg agcctgcgcg ccgccttcga ggcggaacgt gccgtccttg gcgttgtcgt
     5881 tgaacaccgt catccacacg tgcgtcggat cccacgcgag atcgaaacgc ttgttcgggt
     5941 cgctgtcgat gccggtgccg gtcttcttcg ccatgtcttc ggtcatcgtc ggaccgaccg
     6001 gcgacaggat ctgactgagc tcgtgcagca cgttcgccgg cacgaccttc gcgctgttga
     6061 gacgattgat cgcttgcgcg cgctcgttgg cttcttgctc cgacttcttg agatcgtcga
     6121 agccctggag ctgcttttgc ttcgcgctga tttcctgctg cagctgcgcg ttggtttcgt
     6181 tcagcgccgt cagccgcgag cggcggggcg cgtcgacgat cgcgaacaca gcaacggcag
     6241 cgaccgcgag accgacgaca ccgaacacga tgtctttcgt ccccggctcg cgtgcggccg
     6301 cagcagcgcc gcgcatcttg gcgcgcttct gcgggaggag gttgatcttg atcatcggct
     6361 tacttgtctc ccgatgcgcg catcgacagc ccgacgccga tcagcgcctc gggtgaatgc
     6421 gcagccaagt acgtccggtc gagtgacgga tcgatctcga cgcgcttcca cgcatcggca
     6481 acttcgagtg gcaagcgcga gcgacgctcg atcgcgcggt gcagcgacgc gactttcgag
     6541 ctgccaccgg caagcacgat gcgtgtgacg ttcgcgtcgg cggtcgtcgc gaggaagaag
     6601 tcgagtgagc gctggaactc gccggccatg acttccgaca cgccttccat cacgcgaatc
     6661 acttcttgcg gcacgacacc gtcttcggtc tgcgtgccgc cgaccttgta cgcctcggcc
     6721 tcatccgccg cgatacccat ctgcttctga atctcttcgg tgtacgcgtt gccgccgatc
     6781 gtcacgtcgc gcgtgaacag cgacacaccg ctgcgcacga tgttgatgtt cgagatcgcg
     6841 gcgccgatgt tgatcagcac gacggtctcg cgtggatcga gcggatagtt cgcttcgaaa
     6901 ccgttctggc tcgcgaacgc ggcgacgtcg acgacttgcg gcgacagtcc ggcatcgcgc
     6961 acgacctgca cgtaatcgga gacaacttcc ttcttcgcgg cgacgagcaa cagctcggtc
     7021 tgaccgctcg ccgtcatcgg attcgtgacg tgataatcga tctcgacgtc atcacgaccg
     7081 aacggaatgt gatgctcggc ttcgttgcga atgttcgccg cgagctcctc gggccgcatc
     7141 atcggcaccg tgatcttctt gatgattacc gagtgacctg cgatcgcgat cgcgacgtcc
     7201 ttcgtcttca gacggagacg actccacagc tgacggatcg catcggtgac tgcgccttga
     7261 tccatgatcg ttccgtcgac gatcgtttgc ggcacgaggg gctgcatgcc gaacgcttgc
     7321 agcgcccaac tcgagccctt tcgtttgagc tgcaccgcct tcactgctga agagccgatg
     7381 tcgagcccga tgcattgttt agccatggaa ctcctgcggc tgaaaatttt gtcatgccgc
     7441 ggtgcaatct ccaaaaacgc acgcgcttac gtaagccgga acccgctcag gaaatcccgt
     7501 actccttcat cttgtagagt agcgcgcggt ggctgatctc gagcagcttt gctgcgtttg
     7561 tgcggttgcc ctgggttact tcgagtgccc gccggatgag gtcctgctcg agcgatcgcg
     7621 tggccttctt gatggatagc tcgtggtcat cggcctggct cgcactccca cctgcgcgca
     7681 cgtgcatcac cgtcgtgagg aacgccagat cgatggccgg cccgtcggat agcacgagtg
     7741 cgcgctcgat gacgttctcg agctcgcgca cgttgcccgg ccacggctgc ttcgcgagcg
     7801 cttcgatggc cccgtccgac acctcgacgt cgcggttgtg ccgcgccgca tgtcgggcca
     7861 caaagaatcg cacaagctgc ggaatgtcct cggttcgatc gcgcaacgcc ggcaccgcga
     7921 ccggcagcac gttgagccgg taaaacaggt cctcgcgaaa cctgcccgcg gcaacctcgc
     7981 cggccaggtc gcgcagcgtc gcggcgatca ggcgaacgtc caccttgatc ggcgccgagt
     8041 cgccgacccg gcgaatctcg gactcttgca gcgcgcgcaa taacttcgcc tgcagtggca
     8101 gcggcagctc gccgacctcg tcgagaaaca gcgttccacc gtcggcctcc tcgaacaggc
     8161 caactttgtc ccgcaccgcg tcggtgaacg cgccgcgcac gtgcccgaac agctcgctct
     8221 ccaaaagtgt tgccggaatc gcggcacagt tgatcgcgac gaatcgcttg tggcggcgcg
     8281 gcgacgcgtc gtgaatcgcg cgcgcgacca gctctttgcc ggtgccgctc tcgccctgga
     8341 tcagcaccgt cgtgcgcgtc ggcgcgacaa ctttgatcgt cgacttgagc ttctgcatca
     8401 ccgggccgtc accgatgatg tcgggcgcgg ccaggaagcc cttgccgttg ctggttccgt
     8461 tgctgttgcc gttcgcgtcc ttggcgcgcc aggtcgggcg gcgcagtgcg agcgcgagtg
     8521 cgagctcgtc gatcggctcg gtcagaatgt cgtcgacacc ggcttcgagg agcgacgtcg
     8581 cgagcgcgac atcacgcgtg cgcaccaccg cgatcaccgg cggtgcatcg gcgagcgcgc
     8641 acacctgcgg cgcgaggtcg ggaaccgcga tcacgacatc gcaatcgctc gccgcgaagt
     8701 gcttcttcgc agcgtcgagc tctgccgccg cagcgacatt ccagccgtgc ttggcaacga
     8761 gcatgtgcag tgcacgtcgg gcttcgtcgt cgggcccgag cactaacacc cgatgcaccg
     8821 cgccgggtgc tggtgcgctc gtcacgacaa aaatcgtagc acgatggcga ttgcgctctg
     8881 ccaacgcctt ggcgtcgcgg ccgaactacc cacgtaaaaa acgtcactcg gaatttggcc
     8941 gaccgtctgc tctaatagcc cgcgtgcgac ccacgataat gatgcgcgtc gttcctgctg
     9001 ttgccgtgac actcggtatt gcagcgtgcg tcgagccacg ccggcccggc gagcccggtg
     9061 acgatgcgag ccgcgtaacg ctcctcgagc tgaccgagac gccacggacc aagagcgatc
     9121 tattgatcgt gatcgacgat acgaccgcga tggcgcccgt tcgaagccag ctcgcaacgc
     9181 ttccggcacg cctcgcagac cggctcgatt ggtacacgcg gcgatggatc gatctgcggg
     9241 tcgccgtcgc gacgaacgac ggcacgctcc gtcgcctgcc tggcgtcgac acgccctggg
     9301 tcgccgacgc gttcgacttc gattacacac gtcgccgcaa ctacgacggc acgctcgccg
     9361 acgcgctgac agcgctgatg gacgtcgggg ccgcaaaccc gagcacgagc cagccgctcg
     9421 aagcagcgcg ccacgcgctc gaaaccggca ccgagctgat gcgtgacaac gccggcctca
     9481 tggttctcac gatcagcgcg gccgacgatg cctcgtcgtt gcccgtgagc gactacgtga
     9541 actggatgtg gtcggtaagc aacggacaga cctggtggcg cgacgtcctg ctgagcggcc
     9601 tctacgtcca accagcgagc cggctcgacg agtactacaa ggcattcccg tggtcgatca
     9661 tcacgccgct cgatagcggc aacgtcgagg ccgcaattcc gtgggtgccc gaggggagcg
     9721 gcaacggtgg gcagagtgcg gacagctggg tcgggctggg ctgctacgag ctagccgatc
     9781 tcgatgcggc gatccccggg ctcgatcacg agtgccagat gatggtctcg atcaacaacg
     9841 agtggcgcga gctgcccgaa tgcaccgcga gccagcgcat cgagcgcacc gagcgtgagc
     9901 gcggactgtc cggaccaccc gaggcgtgct ggtggctgcg gccgaatttc gagagctgcc
     9961 tctacaccac tgggcgcgag atcgcgctca gcggctacac cggcgctcag cacccagcgc
    10021 tgcgcttcga ttgtcgagct cggtgacgac cgcgtagcgc gagcgcgatg gcacttcgcc
    10081 aacggcgttg gcatcgcggc cgcagtaagc gtgcgaaagg cgtcactccg aaggtggccg
    10141 accgtttgct gtgacggatc gcgtgcgctt cgcgacaccg atcgtcatga cacgcctgct
    10201 gccggccatt gccgtgacgc tcggtactgt cgcatgcgtg gacacgccgc cgttcgccga
    10261 gcccgaggtc gaccagagtc gcgtcgaggt ccgcgagctc ccagcgagtc cgctgtccaa
    10321 gctcgacgtg ctgatcgtcg tcgacgattc gatcgcgatg gcgccgtatc gcgaccgggt
    10381 ccaggcgctc tcgtcgatgc tgacggatac gctcgcaaac ttcgccgagg gctgggcgaa
    10441 cgtccggatc gcggtcacca cgaacgacgg caagctgcgt cgcgtcccga ccacgaacgc
    10501 gccctacgcg atcgatgccc gcgagtacga cttcacgcat cgtgcgaact acatcggtcg
    10561 tctcgctgaa accgtcgcgg cgatggtcga cgtcggcgtc gacggcacag gcccgagcca
    10621 gccactcgca gcgatggtcc acgcgctcga aaccaactcg cagtttctcc gcgatgactc
    10681 cgcgtttgcg gtgctgatga tcggcgcgag cgacgacgcg tcgccgttta ccgtcgacga
    10741 gtacgtaaac tggatgaaac ggctcaccgg cgggttctgg aggcgtcgcg tcgtggtggt
    10801 cgggctctat ccagaatctg ccccgcgcat cgccgagtac tttcgcggca tccacgaggt
    10861 tgggttcgca aagcccatcg actacgacgg cgatctgcgg ctagccctcg tgaacctgct
    10921 ggtcttcgac cggtggaccg atctcgtcgg ctcgccgtgt ctcgaatcga ccccgttgct
    10981 cctcgatccg agtgcagcgg acgaccgtta cgactgcacg atgtccgtcg atctcgaacg
    11041 cgggctcgtc gcagtgccgc agtgcgaccg tgcacgcccg gatggcgaac gaactgacgg
    11101 attcgcgccg cgcgcgacct ctgcatgttg gttcttccgc gcggacccgc agaactgcac
    11161 gttggaaaac catctatccc tcggactcag cggctacacg ctgctgcatc accccgcact
    11221 tcgactggag tgtcgaacca gatgatgaaa tgtgccctca ctgcgctcgt gctcgccgca
    11281 gcgtgcgtcg acacgccgcc gctccaggag cagcacgtcg aatccagtcg ggtgacgatc
    11341 cgcgagattc ctgaaactcc acgcgcgacg accgatctat tgatcgtcgt cgatgacacg
    11401 gtcgggatgg cgccgtacca agcgcagctc gcaacgttgc ccgcgcgcct tgcgagccgc
    11461 atcgactggt acacgcggcg gtggatcgat ctgcgcctcg ccgtcgcgac gaacgacggc
    11521 acgctacggc gtctgcccgg catggacacg ccgtggctcg cggacgcgtt cgacttcgat
    11581 tacacgcgcc gccgcaattt cgacggcacg ctcgccgaca ggctcgccga gctgatggcg
    11641 gttggcaccg cgaacgaagg tccgagccag ccgctcgaag cgggacgtcg cgcactcgag
    11701 accggctccg agctcatgcg cgacaagacc ggcctcatgg tcctgacgat cagcgcaacc
    11761 gatgacgcgt cgacgtggcc ggtcagcgat tacgtggagt ggatgacaaa gctcgccggc
    11821 ggagatttct ggggacgcgg cgtgctcctc agcggcctct acctgcagcc ggcggcccgc
    11881 ctcgacgaat acttcaaggc aacccacgcg atcatcaccc cgctcgacgg cgacaacttc
    11941 gaggcggcga ttccgtggtt ccccgccgga ccgaacacgg tcaccttcgg gtgtagcgaa
    12001 gcggtcgatc tcgatccgac gacaccggca ttcgattacg actgcgcgat gatggcgtcg
    12061 atcaatggca cgtggcgcac ggtgcccgaa tgcacgccga accagcgcga cggcggcgag
    12121 gttcatgacc gcaacatccg cgaacggggg ctggcgggtc cacccgaatc ctgctgggcc
    12181 ttccggccgg atccgcagag ctgcttctac ggagattcca agtcgcagct cgtgatgagc
    12241 ggctacactg catttcaaca ccccgcactg cgattcgatt gccgaagcaa ctgatgaaac
    12301 gcacgctcct cgttctcgca ctctcggctg cctgcaccgg cgatgattcg ggcggcggcg
    12361 tccaaggtac cgcgcacgtc gagacctacg agctgccagc tgcgcttccg ccgaagctcg
    12421 acatcctgtt cgtcatcgac gactcgaccg cgatggcatc gcatcagacg ccgctcgcgg
    12481 cgatgcctgc acagctcgag cagctcgtct ctggcacgta cggtgacgcc gtcaactatc
    12541 acatcggcgt cgtcaccacc gacgccgcga gtggcggcaa cctgcggacg gcggcgggaa
    12601 tcaacggcac gttcatcgtc cacgaaaata cgttcaccgg cgcgacgacc aactaccagg
    12661 gctcgttcgc gaccgcgctg ggctcactgt ttccaagctc tgcaacgagc accgcgagca
    12721 atcagcccct cgcgacgatg cgcgccgcgc tcgagaacac cgtcgcgaac gccggctttt
    12781 tgcgcagtga ggcgtatctc ggcgtcgtca ccatcagtgc gaccgacgac acgtcaggag
    12841 gcggcgcggc agccgactac gcggcgttct tgaaatcgtc caagaccgat ccgacgagtg
    12901 tgatcgtgtc tggcgtgatc gatgcgagtg cgacgcggct caacgcgttt cacgatgagt
    12961 tcccgaatcg caccgcgaag acgtcgatcg caggcaccga ctacggcagc gcgctcgagc
    13021 tgtttggcca gctctatcgg accgtgctcg gctacgcgtg caacaaggag ccagccgatg
    13081 tcgatccgac cacgccgggt ccgcaatacg actgcgcgtt cgtgtgggtc gatcaaatcg
    13141 acgggcatac cgagcaccga ctgcccccgt gcgttggtgg tgacaccagc gggtgctggg
    13201 agatcgtggt ggccgatccg agcatctgca ccgacccgtc gacgaaggcg catctccaga
    13261 cccgcggatt caccacgtct ccgtcgaagt atggtgatcc atttcacccc acgattcggg
    13321 gtcagtgcct cgtcaactga tcgacgatcg aaaccccggc ggggcctttt ccgccaacga
    13381 tcgggtcgat tcgccaacca agttggcggg atatgacgcg cggcgaaaaa gtaagcgttt
    13441 gaaactgctc ggacttcaac ttggcgggcg acttgctcta tgctggccga gcactggagg
    13501 tgcgcctatg cgcaacgccg ttactacgac ttcacttttg ggtcttcttg gacttgggct
    13561 gctcgcgggc tgcccggatc gcaccatctc tgaggtcatg cctcagcagg ggcgcgtcga
    13621 atacaaagac atcccggtca cggtgaaccg tgacatcgac atcttgttca tcatcgacga
    13681 ctcgccgtcg atgctcgaca agcagacgaa cctcaaaaac aacttcccga acttcatcaa
    13741 cgtcctcaac accatcgaag gtggtctccc gaacgtccat ctcggcgtcg cgagctcgga
    13801 cctcggaacg aagggcgcgg ccgacgcgca ggcggcaccg ggcatcggct cgggtccggg
    13861 ctcgtgctcg ggcaacggca agtcgggcaa cctgcaactc ggcaacccgg caagcacgct
    13921 ggtcacgggc aacttcatca gcgacacgaa gaacccggac ggcacgcgta cgacgaacta
    13981 ctccggctcg ctcgcgaacg cgttctcgtc gatcgcgtcg ctcggcgcct cgggttgcgg
    14041 cttcgagcag cacatcgaag ctgcgaagcg cgcgctgaac aacaacccgg cgaacgccgg
    14101 cttcatgcgc ccgaacgcgt acctcgcgct catcttcatc caggacgaag atgactgctc
    14161 gatgaaccac gcgacgctcg tcggctcgga tacgacgacg ctcggacctc tgcagtcgtt
    14221 ccgctgcaac cgcttcggcc acaagtgcac gacgggcggc aacaactcga cggaaatgaa
    14281 cgcggtcggc ccgaagagcg gctgcgcctc cgaagagagc ggcacgtacc tcaccaagat
    14341 cggcgactac gtcacgttct tcaaaggcct caaggccgat ccgaacaacg tgatcgtcgc
    14401 cgcgatctcg ggcccgacga cgccgtacga agtcgagctc cgcacgcctc cgggcggtgg
    14461 cacggcgatt ccggccgtcg cgcactcgtg cacgtatcaa ggtatgaacg gtcccgaggt
    14521 cgccgacccg tcggtgcgcc tcaagcagtt cctcgattcg ttcccgaacc gcagcacgtt
    14581 ctcgacgatt tgccagcagg acctctcggg cgcgctgatc cagatcgccc agctgctccg
    14641 caccgtcatc ggttcgccgt gcatcgaagg caagctcgcc gacgtcgacc cgaccacgcc
    14701 gggcccgcag tacgactgct cggtgtccga cgtgcgcaac tacggcaagg cgaaccagag
    14761 cgaagaagtc ctcccgcagt gcaacaacct cggaaccccg gcctcgtcga cgaacaagcc
    14821 gtgctgggcg atcgagaccg acacgatgaa ctgcaccgct ggcgatcacc tcacgctcaa
    14881 agtcgagcgt aacgaggcgc ccgccgccga cacgcacgtc atctcgtact gcgtgaccga
    14941 agcataaagc acactgctct agtgctaacg gcgcggctaa ctaccgcgcc gtttttgttt
    15001 tcggtgcaac cgcgcgcgtg cggcgctgtc tctttttttt tcggtgatgg atccgcacga
    15061 gcgtcgacac agatcgacat gcggatcaga gaagtgttgt tcgcgttggt gatggcaggt
    15121 tgtgcggatc gcacgatcgc atcggtcaat ccggtgcaag gcaaagtcga aacgttggac
    15181 ctgccgtcga gtctcgagaa agacgtcgac atcttgttct tgatcgacaa ctctggctcg
    15241 atgctcgccg agcagcagtc gctgcaggcg aacttcccca agttcatgca ggtgctcgag
    15301 acgctccagg gcggagcacc gaacatgcac atcggcgtcg cgacgagtga catggggcaa
    15361 cacgccaccg acggaaacgg caccaactcg gcggccggcg gttgcaccgg cgcaggtgac
    15421 gatggcgtgc tgcgcacgac gccggcggtg tcgggccgct tcatcatcga cgaggagagc
    15481 ggcggcacgc gcaaccgcaa ttacagcggc acgctcggcg acgcgttcgc tgcgatggca
    15541 ggcgtcggta ccgacggctg tggcatcgag cagcatctcg gtgccgtcga gcgcgcgctc
    15601 ggaaacacgg ccaacgctgg cttcgtgcgg ccgaacgcca agctcgcagt gatcgtgatc
    15661 gccgacgaag acgactgctc gctcgcgcac aacagcctgt tcaacggcac cacggcgcag
    15721 ctcaacttcc gatgcacgca gaccggcatc acgtgcaccg acaatcctga tctatcgaag
    15781 ccgggcgtgc gcacgatgtg ccagccgaac gatcagtcgc agtatctcga gccggtcgac
    15841 aagtacgtcg acttcatcaa gaccctcaag ccgaactggc gggacgacgt gatcgttgcc
    15901 ggcatcgtcg gtgacgacaa cccgttcgag atcaagctcg acgcgatgaa caacccgatc
    15961 ctcggaccgt cgtgcacgta caacggcggc gatggtgccg tgccggcgct gcgcacgaag
    16021 gacttcctcg agcagttcac gtacaccgtg aagcgctcga tttgcggcgc cgacctctcg
    16081 caagccatgg tcgacatcgc cgcattgctc aagcgttcga tcggcgatcc gtgctgggaa
    16141 ggcgaaatcg ccgatctcga tcccgcaccg ggccttcagg ccgactgttc agtggtcgac
    16201 gtgagggtgc tgcccgacgg cacacgccaa gaggtcgcgc agattccgag ttgcagcgtc
    16261 gccggggggc agatcccgtg ctggcacatc gaggaggatg cgatggagtg tttctacacg
    16321 tcgacgcacc tcaagatcgt cgtagaccgg ggtggcgtga tcccacccgc cgacatgcgc
    16381 ctcaacgcga gctgcgtgac caccgacgat ggtagcggcg gtttcatgta gcggccggcg
    16441 ataggcgata ccggttcctg taactcaggt gatttccgac gagtcttgat gttagtttct
    16501 gcccgtgcct gcgcagggca gacccttgat tacagcgaac caggtgaccc tggctcgctt
    16561 gatccccatg ccgctgttgg cgtggctgat ctatgagggc aaccgcgtcc acaacaacag
    16621 ctatctgtgg ggcgcgctga tcgccggcac actcatcggc tgcaccgact gggtcgacgg
    16681 catgctcgcc cgcaagtacg gcccgaccgt gctcggcggc ctgctcgatc cgatcgccga
    16741 caaggtcttc atcgcgttcg cgtacacgcc gtttgccgat tccggcatgg tcccgtggtg
    16801 ggcgtgcgcg ctgatgttca cgcgcgagtt cttcatcacc gcgctgcgct ccgcctacga
    16861 gcaacgcggt ctcaaattca agaccagcta cctcgcgaag gcgaagacgt ggacgcagat
    16921 gcagggcatc ggcatcttgc tgctgttccc gctgctcgac aacaaagcca tcctgctgtg
    16981 gctgttcgct gccggcgcga cgattccgat tgtcgtgctc gtcgtgtgga agatcgtcaa
    17041 aggcgagtgg tggcgcggcg cgttcgtgat gaccggctcg ttcggcgcct tgctcgccat
    17101 cttcttgatc gacgagagct acgcgccgcg cgcgctcatg atcggcatcg tcggtattac
    17161 atggctcagc ggtctcgatt acatcgtcgg cggctggccg aagctgcgcg cgtacggcga
    17221 cttctcgcgc gccgacttcg tacgtctgct cggctcgctc acgctgccga tcctcttgtt
    17281 cgcggtgctc gtctacaccg atgcgccgcg ctggacgatc ttcgcgatcc tcgcgttcga
    17341 gctcgcggtc ggtggtctcg acaatctgct ctcgcaccac aaggccgcaa cgcttgcgct
    17401 cgcctggggt ggccgcgtgc tcggcgtgtc gctgctactc ggcggcgcgc tgctgatgaa
    17461 ggatcaggcc aaggcgctcg caatcgttgc gtgcgcggtg agcgtgttcg gctgcacggc
    17521 cgaattcatt cgcggccgcg cgtattacat cgatcacctg atggaataac gctggtacgc
    17581 tcgccgcgat gcgacacgcg tggtggctcg tgcttgcagc atgcgggagc agcggctcgt
    17641 cgccggatgc atccagcgat gccgtctgcg agatggactg cgccggcgtc tgcaacggct
    17701 cggcgatgac cgatcgctgc ggcgtctgcg ataccgaccc gacgaacgac tgctggaaag
    17761 gcgtccacgg cctgtcgtcg ctgacgccgc tgcccgacca ggcgaatctt ggcagcggca
    17821 agctcgccta cgaggtcagc tccgtcgcgc agcagcgcgg gttgttctac ggcggcccgg
    17881 cgatgacgat gccggcgctt ggcatcgcgc aggtcacgg
//
LOCUS       DASUFX010000575        14203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1760460, whole genome shotgun sequence.
ACCESSION   DASUFX010000575 DASUFX010000000
VERSION     DASUFX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14203
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760460"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4141
                     /locus_tag="VFV99_23115"
     CDS             <1..4141
                     /locus_tag="VFV99_23115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062288.1"
                     /translation="PAELAPWLDTVLWAPDGDPARMLFRGFAVDTRATRRLARHARKE
                     LRAQKRFFAHAKREIRVLSAEEPHVRAELGVAAGGSCVAQDVFAGLTGEVCIYAEGAT
                     GALAVLLDGRALDRVEFDSPLAFDVVVDSAKVAPGDRYRGVKRDAEYKRVERAMRAGL
                     VRALEAHALSKRDPNEAPIDARLFRKAFGVLSELGLPVRGPLALAPAYRTPDGRWLSL
                     VDVREESVVGIAPPGRRVAVPAGRVVIELDENERRVLSALGKARLVTYRRAEVGNLRS
                     IVTSYAVQGGGAALVVREPGVTAVIAVGQPTRLQLFHLGAELADRSYQPRWVSCTIAI
                     DADAIVPENEWDGIVDDAGLGSRDYGPWELALIRALASAVVGEPPPELVLTQQARLAL
                     DSKPGRALCEAILGHDPHDLLGPELVTKLRTAPLVAQLGELEMTSITHAAERFPGAIP
                     HIEGNAEPVDGFAPLVGPTLVARALARLVVRDIIDATAELQRRHRNALRERRLAAHRF
                     NPPTPWKLPAEDHVELTGKLVRGLMGVWHGPFEIRLFVEGRPFFVIHPGDHDLPIVAA
                     VEIDQTYCNATFDGIDADVRTTISGEVRVGIQALLVAIAKQNPAKLADRGPARTLLVN
                     APELTSSTRLALRAAPAFLTIQGSRISIDEAAQPRMIISVAAWEGEWLGPGDGESASA
                     LDAPILAVPDPTHEISRIIGELHGDSVVDVTADVTRLQSRRRIARGLLPVPRVMDAPA
                     ALKRQLGDLGALGKCVGVGEIALVEDSGSTVRLHEHGSFVRSESIDVLPPVTLAVELA
                     DSIALEKDVQELAIELAKHVLASGPADSLPPRIQRNLMRAALARRIDRDVLAGLSMWQ
                     DFLAQIDKLGNAWVVCEPTELQPLDENRHVFFFERSTFELARDHGWHVVEAMKELQLD
                     AAARKNRARPAAISLELPRTGVLAQALIEGDGVTSPRGVVGVLSPMVAHARGVWPHMK
                     MHPFDRVDDPCRWPTVAVVDDARLSPDRTWQAPLQNEEWQAVAKELRNASERALATYG
                     EIPEDALVSLRINNHACADVGALRKAPKSLIRGVLWLIGEPQRDVGIQVSGVTLSRFV
                     PDHQLALGGKLLVYAPDGLDVTAALQQLCGQTHGKLVRQLIKQDHDKDLIAAHVAHAL
                     AIRTVRANDVRGLDFGCFSPRRLDARALTSLFRREDQITLIHPDTEPDPDPHVIEVVD
                     NDSILSRVVIAHLGSRIRRQRPAPKPRRAATTPPPLPGLPPPKFAPPPPKREPVRAKP
                     PEPPHPLKALVGALRGRLADLGIGGYQWSIVDREEPMFSYSDEIEVAGDNVRLRALAA
                     AQLANSPLTDAGIDVVIAHLVTVLNVALSQITDASEAHAIGVLLANRPSANRPRSRQS
                     S"
     gene            complement(4095..6734)
                     /locus_tag="VFV99_23120"
     CDS             complement(4095..6734)
                     /locus_tag="VFV99_23120"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX5062289.1"
                     /translation="MSESETLRTDLNRVTSALLSAVGTLAGKQSEKLYVQLEDAATHL
                     RHGDLPGGRRAFAQQIETLDDDQLEAMARANALECHLMNTAEERERLRAIRVRGGEPP
                     DGVVAALDGLWEAGFRERDFRAFFERALVMPVITAHPTESRRRSALDHLTKISGLLDN
                     KAQAPLEAEVLALHATEDSRSHRPTPFDEIETALDVFRRSLLEVTPRIYRTIEDRLTA
                     RFGGTWRLPSFLRWGTWVGGDRDGNPNVTAITTRIALQRQRNVVVTRYLADVETLGKF
                     LSMSALRARPKSLDELMASIETDRERFPEVAAHARPRTVHEPWREKLWYMQARLRGAL
                     DYAEHGYSEVSRYRSDLAVLDRSLRDAGFAAIAEQELRDTIRRVDVFGFHLASLDIRQ
                     HSGVHDRVVGELLARGGKPGYLDRDEAGRRALLGDVLGAPVAPIRDRDALTAEAREVL
                     ATLDVVGRARRELGPRACERYVTSFTREVSDLLEVVFLARAAGLAPGELRPVPLLEQL
                     EDLDRAEQIARNMLENPVLRSEINGELEVMVGYSDSGKQVGYVASSMALRRAQSALAQ
                     VTAPTEVALTIFHGRGGALGRGGGPASDAIRAQPAVAVGGRIRVTEQGETVTARYARP
                     AIAERDLELTIAAVLGAAETAREPAPPAADEQHLQTAADAARRAYLELTQDEDRLARY
                     TVAATPIEDVAHLPIGSRPASRAARMSLETLRAIPWVFSWTQSRHGIPGWFGVGTAVE
                     ELVKEIGVDGVRGLCGRSRFFRALVRNCEVSLVRSDIDVAREYSRLADPDAAQIFTLI
                     EAEHTRTVHALRDTLGMTPPLASRPYLVASVERRNPHLDVLSHVQIESLRRRRAGIGD
                     AERLTRIVFTTIGGIAAGLQTAG"
     gene            6779..7141
                     /locus_tag="VFV99_23125"
     CDS             6779..7141
                     /locus_tag="VFV99_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5062290.1"
                     /translation="MPVSTHNGGCHCGAVRYSVEIDLEAPSITCNCSMCGRAGTILSF
                     VPADKFKLEQGEANLTDYQFNKHAIHHVFCKTCGIRSFARGSGPHGPMVAVNLRAIDD
                     IDTFAAVKRAKEFDGKSK"
     gene            complement(7157..7975)
                     /locus_tag="VFV99_23130"
     CDS             complement(7157..7975)
                     /locus_tag="VFV99_23130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5062291.1"
                     /translation="MTTPSGYLHGYSEKERERLHRQARFLEPMVHDRLPFRRRRRLLE
                     VGSGVGAQTEILLRHFPEIQVTGVEINDDQLAEARRYLATVPWATGRYTITKGDATRL
                     EFSPESFDCAFLCWILEHVPMPARVLSEVRRVLVPGAPIVCNEVLNATFFIDPYSPNT
                     LRYWMAFNDHQLELGGDPFIGAKLGNLLQSVGFRDVTTEVKTFHLDNRAPGERAEFLA
                     YWTDLLLSGAPALQKAKQVSQEVVDGMTRELELVARDPNSVFFYSFVQARARAW"
     gene            complement(8001..9851)
                     /locus_tag="VFV99_23135"
     CDS             complement(8001..9851)
                     /locus_tag="VFV99_23135"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HEX5062292.1"
                     /translation="MTAVTQLTIEQLLELHPWPAELKGEKRIERLWVYDLPGTPQALW
                     PFISDTSRMNRALGTAEMTFWEKDGKRFGSSKAGGVKHEWLEVPWNWVAEHWLTSTRI
                     YERGFMRVNFSIQRLEPTPTGTRVYLYFGTIPRGALGAAAIRIGFPTIKRAYDRVLPA
                     LAAELDKAQPHVLQLPPPPLAGDADARLRAGREQLIGEGLPPAIVDKLVDWIRTGDDV
                     DLHRIQIRARAREWKLPEIDLLRTALHATRAGLLDLSWDTICPHCRGVRDENGSLSQL
                     KAEGHCDVCKLDFTTDTAEAVEVTFRVHPSIRDVPELLYCSAEPARKDHILVQRMLSP
                     GEKLEIKPRLASGRYTVWLGHRGGWYLDVDNESPAELVYEDHPEGTVVHASREPLVRV
                     ENDGSEPERFTIERTTWSDDALRPGMLLSFQEFRDLFSEDYIGADVKLAVGEQTLLFT
                     DVVGSTAFYASRGDPAAFVEIKKHFDEVFAIVAKNRGAVVKTIGDAVMATFVNPVDAV
                     RASQQIHQAFHPTRADTPIRLRISLNTGPCIAVRLNANADFFGGTVNIAAKLQAMAEG
                     YQIAMSEATYRSAGVAELLAQEKAELEDLTYESKALKEVVRVKRWSVFRA"
     gene            9866..11779
                     /locus_tag="VFV99_23140"
     CDS             9866..11779
                     /locus_tag="VFV99_23140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062293.1"
                     /translation="MRDRFEMLFEHSIDGVFVMTLDEPIEWHEAADQQALLDYVYEHL
                     RVTAVNRAMCEQMRATREELINSTASQRWGDNGSIWKERMRELYQNGRMYQSLRAPRD
                     DGTWMDVEGQYVCMYDEQGRITGHFGTQRDVTEKLKVIERLELAMVGGDTGIWDHDLA
                     THELYYDARFIERLGYSANDPTVRRPEWWGSIMHPSDLINARRAFEAHIRGESPLYRC
                     EARLRKANGEWMWIMWSGRVTRDGQRRPVRVVGVSVDITERKVLQERLFLAERMASIG
                     TLAAGVGHEINNPLTYIVLNLTLLDRELRNLDLPATSHAKATHMIEQARYGTERVRRI
                     VRDLQALTRVPDENLTRVDLVSVIDRCLEIADHEIRHRARVVRELGELPAVLGSEGRL
                     VQLFLNLLVNAAQAIPVGSADTNLIKVVATTWNGQAAIMITDSGVGIAPDALGRIFDP
                     FYTTKPIGEGTGLGLSICRSIVSAMGGEIEVDSALGQGTTFRVLLPAAPAEVVIPPAP
                     VQAEKRCPKKRVLVIDDEPMVGQLVTKVLAEHEVVSETSARSALTLLGSDPRFDLILC
                     DVMMPEVSGMDFYARLAPELRCKIVFLSGGAFTDRAQTFLDSVPNRRLSKPFDVDALT
                     AVLLSSGDHDALP"
     gene            11757..13874
                     /locus_tag="VFV99_23145"
     CDS             11757..13874
                     /locus_tag="VFV99_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5062294.1"
                     /translation="MTTTHYRSCPLCEATCGVAIEVDGDRVVSVRGDDADPFSKGYIC
                     PKGTALADLHHDPDRLRSPVRRDGDTWTPIGWDEALDYVAGKLREIRATHGPHAIAVY
                     QGNPSAHNLGLMTYGQLALRTLGTKNMYSATSLDQLPHMLAALQMFGNQILMPVPDVD
                     RTDLMICLGANPLASNGSLMTAPDIRGRLKSIRDRGGRVIVLDPRRTETAEKADRHLF
                     IRPGTDAVLLLAMIHVLFAEKLVRLGRLAVHGLEDLRTAAAAWTPERAADISGISAEE
                     IRELTRALATTERAVLYGRVGVCVQEFGGLSAWLCYAINALTGHLDEPGGLMFTTPAV
                     DPLPLAGLLGFDGGFARWKSRVSGKPEFGGEMPMTVLAEEILTPGEGQIRALITSAGN
                     PALSAPGGAELERAFASLDFMVSIDPYINETTRFAHVILPPTSPLERSHYDAALNTFA
                     VRNVAKYSPPLFERGADERHDWEIMAGLFTRLKLPIASRATEKLLGRLGPEAIIDIGL
                     RTGPYGLRRGRKALSLAKLRDQPHGIDLGPLESRLPGRLGTRDRKVHVAPKIYLDDLM
                     RLARRFAQPETGLVMIGRRHLRSNNSWMHNSERLVKGPDRCTLMIHPDDAARRGLLDG
                     STARVATTKGAIDLPVEITDAMMPGVVSVPHGWGHGRRGVKLRVAASVPGASVNDVID
                     SSRIDELSGTSALTGQPVEVTRV"
     gene            13871..>14203
                     /locus_tag="VFV99_23150"
     CDS             13871..>14203
                     /locus_tag="VFV99_23150"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX5062295.1"
                     /translation="MSERGPELFAKIGEAKLREVITDFYSRIFGDVMIGFLFEGKNRQ
                     HLIDREYEFTANLLGADVKYTGRPMRTAHAQSPIFGGHFERRLQILRETLRDNAVDPD
                     VQQVWIEHT"
BASE COUNT         2355 a         4750 c         4712 g         2386 t
ORIGIN      
        1 tccggcagag ctcgcaccgt ggctcgacac cgtgttgtgg gctcccgacg gagatccggc
       61 gcgcatgctg tttcgcgggt ttgccgtcga cacgcgcgcc acccgccggc tcgcgcgtca
      121 cgcacgcaag gagctgcggg cgcagaagcg attcttcgcg cacgcgaagc gcgagatccg
      181 cgtgctgtca gcagaggagc cgcacgttcg tgcggagctc ggagtcgcgg cggggggcag
      241 ctgcgtcgca caggacgtgt ttgccgggct gaccggcgag gtgtgcatct acgccgaagg
      301 cgcgaccggt gcgctcgcgg tgttactcga tggccgcgcg ctcgatcgcg tcgagttcga
      361 cagtccgctc gcattcgatg tcgtcgtcga ctcggcgaag gtcgcgcccg gtgatcgata
      421 tcgcggcgtg aagcgcgatg ccgagtacaa gcgcgtcgag cgcgcgatgc gggccgggct
      481 cgtgcgggca ctcgaagcac atgcactctc gaagcgagat ccgaacgagg cgccgatcga
      541 cgcgcggttg ttccgcaaag cgttcggcgt gctgtccgag ctcggtttgc cagtgcgcgg
      601 gccactcgct ctcgcgccgg catatcgcac accggatggc cgctggctgt cgctcgtcga
      661 cgtgcgcgag gagagcgtgg tcggcatcgc gccgccgggc aggcgcgtcg cggttcccgc
      721 cggtcgcgtc gtcatcgagc tcgacgagaa cgagcgccgg gtgctgtccg cgctcggcaa
      781 ggcgcgcctc gtgacctatc gccgcgccga ggtcggaaac ctgaggtcga tcgtgacgag
      841 ctacgcggtg caaggtggcg gcgccgcact cgtcgtgcgc gaaccaggcg ttaccgccgt
      901 gatcgcagtt ggacaaccca cgcgcctaca gttgtttcat ctcggcgccg agctcgccga
      961 tcgctcgtac cagccgaggt gggtgtcgtg cacgatcgcg atcgacgccg acgcgatcgt
     1021 tcccgagaac gagtgggacg gaatcgtcga tgacgccggc ttaggttcgc gcgactacgg
     1081 gccatgggag ctcgcgttga tccgtgcgct cgcgagtgcg gtcgtcggtg aaccgccgcc
     1141 cgagctcgtg ttgacgcaac aggcgcggct cgcgctcgac agcaagcccg ggcgtgcgct
     1201 ctgcgaagca attctcgggc acgacccgca cgatctgctc ggtccggagc tcgtcacgaa
     1261 gctgcgtact gcaccgctcg tggctcagct cggtgagctc gagatgacca gcatcaccca
     1321 cgccgcggag cggttccccg gcgcgattcc ccacatcgag ggcaatgcag aaccggtgga
     1381 cggcttcgcg ccgctcgtcg gcccgacgct tgtcgcccgt gccctcgcgc ggctcgtcgt
     1441 gcgcgacatc atcgacgcga cggccgagct gcaacgtagg catcgcaatg ctctgcgcga
     1501 gcgccgcctc gcagcgcatc ggttcaaccc gcccacacct tggaagttgc ctgccgagga
     1561 tcacgtcgag ctgaccggca agctcgtgcg cggattgatg ggtgtgtggc atggcccatt
     1621 cgagatccgc ctattcgtcg aaggacggcc attcttcgtc attcacccgg gtgatcacga
     1681 cctgccgatc gtcgcggctg tcgagatcga tcagacgtac tgcaatgcca cgttcgacgg
     1741 catcgacgcc gacgtccgga ccaccatctc gggcgaggtg cgggtcggca tccaggcgct
     1801 gctcgtcgcg atcgcgaagc agaacccggc gaagctcgcc gaccgcgggc ctgcgcgcac
     1861 gctactcgtc aatgcgccgg agctcacgag ctcgacacgt ctcgcgctcc gcgctgcgcc
     1921 cgcgtttctc acgatccaag ggtctcgcat ctcgatcgac gaagcagcgc agccgcgaat
     1981 gatcatctcg gtcgcagcct gggaaggcga gtggctcgga ccgggcgacg gtgagtctgc
     2041 atctgcgctc gacgcgccga tcctggccgt gcccgaccct acgcacgaga tctcgcgcat
     2101 catcggtgag ctccatggcg attcggtcgt cgacgtcacg gcggacgtga cgcggctgca
     2161 atcgcgccgg cggatcgcgc gggggctgtt gccggtgcca cgcgtcatgg atgcgccggc
     2221 cgcgttgaag cgtcagctcg gcgacctcgg tgccctgggt aagtgcgttg gcgtcggtga
     2281 gatcgcgctc gtcgaggaca gtgggtcgac cgtgcggctg cacgagcacg gcagcttcgt
     2341 ccgcagcgaa tcgatcgacg tgctgccgcc cgtcacgttg gcggtcgagc tcgccgactc
     2401 gatcgcgctc gagaaagacg tgcaggagct cgcgatcgag cttgccaagc acgtgctcgc
     2461 gtcaggaccg gccgactcgt tgccaccgcg gatccagcgc aacctgatgc gcgctgcgct
     2521 cgcgcgccgg atcgatcgcg acgttctcgc cggcctttca atgtggcagg acttcctcgc
     2581 gcagatcgac aagctcggta atgcgtgggt cgtctgcgag ccgaccgagc tgcaaccgct
     2641 cgacgagaac cggcacgtgt tcttcttcga gcgcagtacg ttcgagctcg cacgcgatca
     2701 cggctggcac gtcgtcgaag cgatgaagga gctccagctc gatgcggccg cacgcaagaa
     2761 ccgcgcacgc ccggccgcca tatcgctcga gctaccgcgc acgggcgtgc tcgcgcaggc
     2821 tctcatcgaa ggcgatggcg tgacgtcgcc acgtggcgtc gtcggcgtgc tgtcgccgat
     2881 ggtcgcccac gcgcgcggcg tgtggccaca catgaagatg cacccgttcg atcgcgtcga
     2941 cgatccgtgc cggtggccga cggtcgcggt cgtcgacgat gcgcggctct cgccggatcg
     3001 cacgtggcaa gcgccgctgc agaacgagga atggcaggcg gtcgcgaagg agctgcgcaa
     3061 cgcgagcgag cgtgcgctcg ccacgtacgg cgagattccc gaggacgcgc tcgtcagtct
     3121 gcgcatcaac aatcacgcgt gcgcggacgt cggcgcgctg cgcaaggcac cgaagtcgct
     3181 gattcgcggc gtgttgtggc tgatcggcga gccacaacgc gacgtcggta tccaggtctc
     3241 gggtgtcacg ctgtcccggt tcgtcccgga tcatcagctc gcgctcggcg gcaagctgct
     3301 cgtctatgcg cccgatggcc tcgacgtcac ggctgcgctc cagcagctgt gcggacagac
     3361 ccacggcaag ctcgttcgcc agctcatcaa gcaggaccac gataaggatc tgatcgccgc
     3421 gcacgtcgcg catgcgcttg cgatccgcac ggtgcgtgca aacgacgtcc gcgggctcga
     3481 cttcggatgc ttctcgccgc gccgactcga cgcgcgcgca ctcacgtcgc tgtttcgccg
     3541 cgaggatcaa atcaccctga tccatcccga cacggaaccg gatcctgatc cgcacgtgat
     3601 cgaggtcgtc gacaacgaca gcatcctgtc gcgcgtcgtg attgctcacc tcggctcgcg
     3661 catacgccgg caacgtcctg cgccaaagcc gcggcgtgca gcgacgacgc caccaccgtt
     3721 gccaggtctg ccgccaccca agttcgcccc gccgcccccg aagcgcgagc cggtcagggc
     3781 gaagccgccc gagccgccgc atccgctgaa ggcgctggtc ggtgcattgc gcggccggct
     3841 cgccgatctc ggcatcggcg gttatcagtg gtcgatcgtc gatcgagaag agccgatgtt
     3901 ctcgtactcc gatgagatcg aggtcgcggg tgacaacgtt cgcctccgcg cgctcgccgc
     3961 cgcccagctt gcaaacagtc cgctcaccga cgcaggcatc gacgtcgtca tcgcgcacct
     4021 cgtgaccgtg ctcaacgtcg cgctgtccca gatcacggac gcgagcgagg cgcacgcgat
     4081 cggcgtgttg ctcgctaacc ggccgtctgc aaaccggccg cgatcccgcc aatcgtcgtg
     4141 aacacgatgc gcgtcaagcg ctcggcgtcg ccgatgccgg cgcggcgcct gcgcagcgat
     4201 tcgatctgca cgtgcgacag cacgtcgagg tgaggattgc gtcgctcgac ggacgcgacg
     4261 agatatggcc gcgacgcaag cggaggtgtc atgccgagcg tgtcgcgcag cgcgtgtacg
     4321 gtgcgcgtgt gctcggcttc gatcagcgtg aagatctggg cagcatccgg atcggcgagc
     4381 cgcgagtact cgcgcgcgac atcgatgtcc gagcgtacga gcgagacctc gcagttgcgg
     4441 acgagcgcgc ggaagaatcg cgagcggccg cacagtccgc gcacgccgtc gacgccaatc
     4501 tccttgacga gttcctcgac ggcggtgccg acgccgaacc aacccgggat gccgtgccgg
     4561 ctctgcgtcc acgagaacac ccacgggatc gcgcgcaacg tctcgagcga catgcgcgcc
     4621 gcacgcgacg ccggtcgcga tccgattggc aagtgcgcta cgtcctcgat cggcgttgcc
     4681 gccacggtgt agcgagcgag gcgatcctcg tcctgcgtga gctcgaggta cgcgcgccgc
     4741 gcggcatcgg ctgccgtctg caggtgttgt tcgtccgcgg cgggcggcgc gggctcgcgc
     4801 gctgtctcgg cggcgccgag tacggccgcg atcgtcaatt cgaggtcgcg ctcggcgatc
     4861 gccggccggg cgtagcgtgc cgtgacggtc tcgccttgct cggtcactcg gattcgtccg
     4921 ccgacggcga cggcgggttg cgcgcgaatt gcatcactcg ccggaccgcc accgcgaccg
     4981 agtgcaccgc cgcggccgtg aaagattgtc agcgcgacct ccgtcggcgc cgtcacctgc
     5041 gcgagtgcgc tctgcgcgcg acgcagtgcc atcgatgacg cgacatagcc aacctgcttg
     5101 cccgagtcgg agtagccgac catcacttcg agctcgccgt tgatctcgct gcgcaggacc
     5161 gggttctcga gcatgttgcg cgcgatctgt tcggcgcggt cgaggtcttc gagttgctcg
     5221 agcagcggca ccgggcgcag ctcgccgggc gcgagacccg ctgctcgcgc gaggaatacg
     5281 acctcgagca ggtcgctcac ttcacgcgtg aagctggtga cgtagcgctc gcaggcacgc
     5341 ggcccgagct cgcggcgtgc gcgtcccacc acgtcgagcg tggcgagcac ttcacgcgct
     5401 tcggctgtga gcgcatcgcg atcgcgaatc ggtgccacgg gggcgccgag cacgtcgccg
     5461 agcagcgccc gccgaccggc ctcgtcgcga tcgagatagc cgggcttgcc accgcgcgcg
     5521 agcaactcac cgacgacgcg atcgtgcacg cccgagtgct ggcggatatc gagcgacgcg
     5581 agatgaaagc cgaacacgtc gaccctgcga atcgtgtcgc gcagctcctg ctccgcgatc
     5641 gcggcgaagc cggcgtcacg cagacttcga tcgagcaccg cgaggtcgct gcgatagcgc
     5701 gagacctcgg agtagccatg ttcggcgtag tcgagtgcgc cacgcaggcg cgcctgcatg
     5761 taccagagct tctcgcgcca cggctcgtgc acggtgcgcg gccgcgcgtg cgctgcgacc
     5821 tcggggaatc gttcgcgatc ggtctcgatc gatgccatca gctcgtcgag cgacttcggg
     5881 cgcgcgcgca gtgccgacat cgacaagaac ttgccgagcg tctcgacatc cgcgaggtag
     5941 cgcgtcacga ccacgttgcg ctgccgctgc agcgcgatgc gtgtcgttat cgcggtgacg
     6001 ttcggattgc cgtcacgatc gccgccaacc cacgtgcccc agcgcaggaa cgacggcagc
     6061 cgccatgtcc ctccaaaccg cgcggtcagt cgatcttcga tcgtgcgata gatgcgcggc
     6121 gtgacctcga gcagcgagcg ccggaacacg tcgagtgcgg tctcgatctc gtcgaacggc
     6181 gtcggtcgat gcgagcgcga atcctcggtg gcgtgcagcg cgagcacctc tgcttcgagc
     6241 ggcgcctgcg ccttgttgtc gaggaggccg ctgatcttcg tcaggtgatc cagcgccgag
     6301 cgccgccgcg attccgtcgg gtgcgcggtg atcaccggca tcacgagcgc gcgctcgaag
     6361 aacgcgcgga agtcgcgctc gcgaaagccc gcttcccaca atccgtcgag cgcagcgacg
     6421 acgccatccg gcggctcgcc gccgcggacg cgaatcgcgc gcaaccgctc gcgctcctcg
     6481 gcggtgttca tcaagtgaca ctcgagcgcg ttcgcacgtg ccatcgcttc gagctggtcg
     6541 tcgtcgagcg tttcgatctg ctgcgcgaac gcccgccggc cacctggcaa atcgccgtgg
     6601 cgcagatgcg tcgcggcgtc ttcgagctgg acgtacagct tctcgctctg cttgccggcg
     6661 agtgtgccga cggcagagag cagcgccgag gtcactcggt tgagatcggt ccggagcgtt
     6721 tccgattcgc tcacggatgg gttctaccgc ggaacgcttc tcggagtagc ctccaggcat
     6781 gcctgtatcc actcacaatg gcggctgcca ctgcggtgct gttcgctact cggtcgagat
     6841 cgatctcgaa gcaccttcga tcacctgcaa ctgctcgatg tgcgggcgcg cagggacgat
     6901 cttgtcgttc gtgcccgcag acaagttcaa gctcgagcaa ggcgaggcga acctgaccga
     6961 ctaccagttc aacaagcacg ccattcacca cgtgttctgc aaaacctgcg gcatccggtc
     7021 gttcgcgcgc ggtagcggcc cgcacgggcc catggtggcc gtcaacctac gtgcgatcga
     7081 cgacatcgat accttcgccg ccgtcaagcg cgcgaaagag ttcgacggca agagcaagta
     7141 gaccgagcgt tgcttttcac cacgcgcgag cgcgcgcctg aacgaacgag tagaagaaca
     7201 cgctgttcgg gtcgcgcgcg acgagctcga gctctcgcgt catcccatcg accacctctt
     7261 gcgagacctg ctttgccttc tgcagtgccg gcgcaccgga gagcaacagg tcggtccagt
     7321 aggcgaggaa ctcggcgcgc tcgccagggg cgcggttgtc gaggtggaac gtcttcacct
     7381 cggtggtcac atcgcggaag ccgaccgact gcaacaggtt gccgagcttc gcgccgatga
     7441 acggatcacc gccgagctcg agctggtgat cgttgaaagc catccagtag cgcagcgtgt
     7501 tcgggctgta tggatcgatg aagaacgtcg cgttcagcac ctcgttgcac acgatcggcg
     7561 cgccgggcac gagcacgcgg cggacctccg acagcacacg cgcaggcatc ggcacgtgct
     7621 cgaggatcca gcacaagaac gcgcagtcga agctctcggg cgaaaactcg agccgcgtcg
     7681 catcgccttt cgtgatcgtg taccgaccgg tcgcccacgg caccgtcgcc aggtacctgc
     7741 gtgcttcggc gagctggtcg tcgttgatct cgacgccggt cacttggatc tccggaaagt
     7801 gacgcagcag gatctccgtc tgcgcaccga caccagaccc gacttcgagc aagcgacgcc
     7861 ggcggcggaa cggcagccga tcgtgaacca tcggttcgag gaaccgggcc tgccggtgca
     7921 gacgctcgcg ctccttctcg ctgtagccgt ggaggtaacc gcttggcgtc gtcatgacgc
     7981 ggagagtacg gcaggtgtag ctaggcgcgg aagacgctcc agcgcttcac gcgcaccact
     8041 tccttgagcg cctttgactc gtacgtcagg tcttcgagct cggccttctc ttgcgcgagg
     8101 agctcggcga cgccggccga gcggtacgtc gcttcgctca tcgcaatctg atagccctcg
     8161 gccatcgcct gcaacttcgc cgcgatgttg acggtgccgc cgaagaagtc ggcgttcgcg
     8221 ttgagccgta ccgcgatgca cggaccggtg ttgagcgaga tgcgcagccg gatcggcgtg
     8281 tccgctcgcg tcggatgaaa cgcctgatgg atctgctgac tcgcgcgtac ggcatccacg
     8341 ggattgacga acgtcgccat cacggcgtca ccgatggtct tgacgacggc gccgcgattc
     8401 tttgccacga tcgcgaacac ctcgtcgaag tgcttcttga tctcgacgaa cgctgccgga
     8461 tcaccgcgcg acgcatagaa cgccgtcgag ccgacgacat cggtgaacaa gagcgtctgc
     8521 tcgccgactg cgagcttgac gtcggcgccg atgtagtctt cgctaaacag atcgcggaat
     8581 tcctggaagc tgagcagcat cccaggccgg agtgcgtcgt cgctccaagt agtccgttcg
     8641 atcgtgaagc gctcgggctc gctgccgtcg ttctccactc gcacgagcgg ctcgcgactc
     8701 gcgtgcacga ccgtgccctc ggggtgatct tcatagacga gctccgccgg cgattcgttg
     8761 tcgacgtcga gataccagcc gccgcgatgg ccgagccaca ccgtgtagcg gccgctcgcg
     8821 aggcgcggct tgatctcgag cttctcaccg ggagagagca tgcgctgcac gagaatgtga
     8881 tctttacgcg ccggttccgc actgcagtac agaagctccg gtacgtcgcg aatcgatgga
     8941 tgcacgcgga acgtcacctc gaccgcttca gcagtgtcgg tcgtgaagtc gagcttgcac
     9001 acgtcgcaat gcccctcggc cttcagttgc gagagcgagc cgttctcgtc gcgtacgccg
     9061 cggcagtgcg ggcagatcgt gtcccacgac aagtccaaga gcccggcgcg cgttgcgtgc
     9121 agcgcggttc gcagcagatc gatctcgggc agcttccact cgcgcgcgcg tgcgcgaatc
     9181 tggatgcggt gaaggtcgac gtcatcgccg gtgcgaatcc aatcgacgag cttgtcgaca
     9241 atcgctggcg gcaacccctc accgatcaac tgctcgcggc ctgcacgcag ccgtgcgtcg
     9301 gcgtcgccgg caagcggtgg cggcggtagc tgcaacacgt gcggctgcgc cttgtcgagc
     9361 tcggcagcga gtgcgggtaa gacgcgatcg taggcgcgct tgatcgtagg aaatccgatg
     9421 cgaatcgccg cagcaccgag cgcgccgcgt gggatggttc cgaaatacaa gtagacgcgc
     9481 gtgccagtcg gcgtcggctc gaggcgctgg atcgagaagt tcacgcgcat gaagccgcgc
     9541 tcgtagatgc gcgtcgaggt tagccagtgc tcggcgaccc agttccacgg gacttcgagc
     9601 cactcgtgtt tgacgccacc cgccttcgag ctgccgaagc gcttgccgtc cttctcccag
     9661 aacgtcatct cggcggtgcc gagtgcacga ttcatgcgcg aggtatccga aatgaacggc
     9721 cacagcgcct gtggcgtacc cggcaggtca tacacccaca gccgctcgat tcgcttttcg
     9781 cctttgagct cggcgggcca cggatgcagc tcgaggagct gctcgatcgt cagctgcgtc
     9841 accgccgtca tggtaccaat gatccgtgcg cgatcgcttc gagatgctgt tcgagcacag
     9901 tatcgacggt gttttcgtga tgacactcga cgaaccgatc gagtggcacg aggcggccga
     9961 tcagcaagcg ctgctggatt acgtttacga gcacctgcgg gttaccgcgg tcaatcgcgc
    10021 gatgtgcgaa cagatgcgcg cgactcgcga ggagctcatc aacagcacgg cgtcgcagcg
    10081 ttggggcgac aacgggtcca tctggaagga gcgcatgcgc gagctctacc agaacggccg
    10141 catgtatcag agcctgcgcg cgccgcgcga cgacggcacg tggatggacg tcgagggtca
    10201 gtacgtgtgc atgtacgacg agcaggggcg gatcaccggc cacttcggga cgcagcgcga
    10261 cgtcaccgag aagctcaagg tcatcgagcg cctcgagctc gcaatggtcg gcggcgacac
    10321 cgggatctgg gatcacgatc tcgcgacgca cgagctgtac tacgacgcta ggttcatcga
    10381 acgcctcggc tactcggcga acgatccgac cgtacgccgc cccgaatggt ggggctcgat
    10441 catgcatccg tccgacctca tcaacgcccg ccgggcgttc gaggcacata tccggggcga
    10501 gtcgccgctg tatcgctgcg aggctcggct gcgcaaagca aacggcgaat ggatgtggat
    10561 catgtggagt ggtcgcgtca cgcgcgacgg acaacggcgg cccgtgcgcg tcgtcggtgt
    10621 ctcggtggac atcaccgagc gaaaggtgct ccaggagcgt ttgtttctcg ccgaacgcat
    10681 ggcgtcgatc ggtacgttgg ccgcaggcgt tggccacgag atcaataatc cgctgacgta
    10741 catcgtgctg aacctgacgt tgctcgaccg cgagctgcgc aacctcgact tgcctgccac
    10801 ctcgcatgcg aaagcaacgc acatgatcga gcaggcgcgc tacgggaccg agcgcgtcag
    10861 gcggatcgtg cgcgatctgc aagcgctcac gcgtgtgccc gacgagaacc tgacgcgcgt
    10921 cgacctggtg tccgtgatcg accgctgcct cgaaatcgct gatcacgaga tccgccaccg
    10981 cgcgcgcgtc gttcgcgagc tcggcgagct gcctgcggtg ctcggtagcg aaggccggct
    11041 cgtccaactg ttcctcaact tgctcgtcaa tgcggcacaa gcgattccgg tcggtagcgc
    11101 cgacaccaat ctcatcaagg tcgtggcgac cacgtggaac ggacaggcgg caatcatgat
    11161 caccgacagc ggtgtcggca tcgcgcccga tgcgctcggg cgcatcttcg atccgttcta
    11221 cacgacaaaa cctatcggcg aaggaacggg gctcgggctc tcgatttgtc gcagcatcgt
    11281 cagcgcgatg ggcggcgaga tcgaagtcga cagcgcgctc gggcagggca ccacgtttcg
    11341 cgtgctcctg ccggcggctc cggctgaagt cgtgatcccg ccggcgcccg tgcaagccga
    11401 gaagcggtgc ccgaagaagc gcgtgctcgt gatcgacgac gagccgatgg tcggccagct
    11461 cgtgaccaag gtcctcgccg agcacgaagt cgtgtcggag acctccgcgc gttcggcgct
    11521 cacgctgctc ggcagcgacc ctcgcttcga tctgatcctc tgcgacgtga tgatgccaga
    11581 ggtgtccgga atggacttct acgcgcggct cgcacccgag ctgcgctgca agatcgtgtt
    11641 cctcagcgga ggtgcattca ccgaccgcgc tcagacgttc ctcgacagcg ttccgaatcg
    11701 ccggctgtcc aaaccgtttg acgtcgacgc gctcaccgcg gtgctactct cctccggtga
    11761 ccacgacgca ctaccgtagt tgtccgctct gcgaggcgac ctgcggcgtc gcgatcgaag
    11821 tcgacggcga ccgggttgtc tcggtgcgcg gcgatgacgc cgacccgttc agcaaggggt
    11881 acatctgtcc caagggcacg gcgctcgctg atctgcatca cgatccggat cggttgcgct
    11941 cgccggtgcg ccgcgatggc gacacgtgga cgccgatcgg ctgggacgaa gcgctcgatt
    12001 acgtcgccgg caagctgcgc gagatccgag ccacgcacgg cccgcatgcg atcgctgtct
    12061 atcaaggcaa cccgagcgcg cacaacctcg gcctcatgac gtacggccag ctcgcgttgc
    12121 gcacgctcgg cacgaagaac atgtactcgg cgacgtcgct cgatcagctg ccgcacatgc
    12181 tcgcggcgct gcagatgttc ggtaaccaga tcctcatgcc ggtgccagac gtcgaccgca
    12241 cggatctgat gatctgcctc ggcgcgaatc cgctcgcgtc gaacggcagc ttgatgaccg
    12301 cgcccgacat tcgcgggagg ctcaaatcga tccgcgatcg aggtggtcgt gtgatcgtgc
    12361 tcgatccgcg ccgcacggag accgcagaga aggcggaccg ccacctgttc attcgccccg
    12421 gcaccgacgc ggtgttgctg ctcgcgatga tccacgtgct gttcgcggag aagctcgtgc
    12481 ggctcggccg tctcgcagtg cacgggctcg aggacctccg caccgccgcc gcggcatgga
    12541 cgccggagcg cgccgccgac atcagcggga tttccgccga ggaaattcgc gagctcacgc
    12601 gcgcgctcgc gacgaccgaa cgcgcggtgc tctacggccg cgttggcgta tgcgtgcagg
    12661 agttcggcgg gctttcggcg tggctctgct acgcgatcaa cgcgctgacc ggtcatctcg
    12721 acgagccggg cggcctcatg ttcacgacac ccgcggtcga tccgctgccg ctcgccggct
    12781 tactcgggtt cgacggcggc ttcgcgcgct ggaagagccg cgtgtcgggc aagcccgagt
    12841 tcggcggcga gatgccgatg accgtgctcg ctgaagagat cctcacgccg ggcgaggggc
    12901 agatccgggc gctgatcacg agcgctggca atcccgcgct gtcggcgcct ggtggtgccg
    12961 agctcgagcg cgcattcgca tcgctcgact tcatggtgtc gatcgatcca tacatcaacg
    13021 agacgacgcg gttcgcccac gtcattctgc cgccgacgtc gccgctcgag cgttcgcact
    13081 acgatgcggc gctcaatacg ttcgcggtgc gcaacgtcgc gaagtattcg ccgccgttgt
    13141 tcgagcgcgg cgcggacgaa cgtcacgact gggagatcat ggccggcttg ttcacacgcc
    13201 tgaagttgcc tatcgcgtcg cgtgcgaccg agaagctgct cggcaggctc gggcccgagg
    13261 ccattatcga tatcgggttg cgcacgggtc cgtacggatt gcgccgcggc cgcaaagcgc
    13321 tctcgctcgc gaagctacgc gaccagccgc acggtatcga cctcggtccc ctcgaatcgc
    13381 gattgccggg ccggctcggc acgcgcgatc gcaaggtaca cgtcgcgccg aagatctatc
    13441 tcgacgatct gatgcggctc gcgcgccgat tcgcgcaacc cgagaccggt ctcgtgatga
    13501 tcggccgccg ccacctgcga tcgaacaact cgtggatgca caacagcgag cggctcgtga
    13561 agggtccaga ccgctgcacg ctcatgatcc atcccgacga tgctgctcgg cgcggtctcc
    13621 tcgacggcag caccgcgcgc gtcgcgacga cgaaaggcgc gatcgatctg ccggtcgaga
    13681 tcaccgacgc gatgatgccg ggcgtcgtca gcgtgccgca cggttggggc cacggccgcc
    13741 gcggtgtgaa gctacgggtt gcggccagtg tgcccggcgc gagcgtcaat gacgtgatcg
    13801 attcgtcgcg gatcgacgag ctgtccggca cgagcgcact gaccggtcag cccgtggagg
    13861 tcacgcgagt gtgagtgaac ggggccccga gctgttcgcg aagatcggtg aggcgaagct
    13921 gcgcgaggtg atcaccgact tctactcgcg catcttcggc gacgtgatga tcggcttttt
    13981 gttcgagggc aagaatcgcc agcatctgat cgatcgcgag tacgagttca ccgcgaatct
    14041 gctcggcgcc gacgtcaaat acaccgggcg gccgatgcgc accgcgcacg cgcagtcccc
    14101 gatcttcggc ggccacttcg agcgccggct ccagatcctg cgcgagacgc tgcgcgacaa
    14161 cgccgtcgac cccgacgtcc agcaggtgtg gatcgagcac acg
//
LOCUS       DASUFX010000576         7320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_787931,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000576 DASUFX010000000
VERSION     DASUFX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787931"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2445
                     /locus_tag="VFV99_23155"
     CDS             <1..2445
                     /locus_tag="VFV99_23155"
                     /inference="COORDINATES: protein motif:HMM:NF003621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX5062296.1"
                     /translation="LATDGPLSATELDKLDAYWRALNYLSVGQIYLLDNPLLKETLKR
                     EHIKPRLLGHWGTSPGLNMLCTHLNRVIKRDDLNMIYIIGPGHGGPSLVAHAYLEGTY
                     SEYYPNVSQDAEGMRRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYALSHAYGAAFD
                     NPDLIVACVVGDGEAETGPLATAWHGNKFLNPQRDGAVLPILHLNGYKIANPCYLARI
                     PKDELTKLMEGLGYKPYFVEGDDPALVHPLMAETLEKIVADIRDIWRDARERGKVKRP
                     YWPMIVLRTPKGWTCPHEIDGKLTEGYWRSHQVPMGDMDKAEHIQVLERWMKSYRPDE
                     LFDAKGRLRHELVVGAMPHGDKRMSANPHANGGMILRDLRLPDFRDYAIEVTSPGGVM
                     GESTRRQGRYLRDVMKMNLASQNLRLFSPDENNSNRWGDVLEVTNRCYMAEIYPEDDH
                     LSPDGRVMEVLSEHQCQGWLEGYLLTGRHGFMSSYEAFIHIVDSMFNQHAKWLKACKH
                     IPWRRPIGSLNYLLSSHVWRQDHNGYSHQDPGFLDLVANKGSDLVRIYLPPDANTLLV
                     ITDEIMRSRDMVNVIVAGKQPQPQWLTMDQAIKHCYSGIGIWDWASNDFNDQPDVVMA
                     CCGDVPTLETLAAVSLARRHLPNLKIRVVNVVDLLRLQSNQEHPHGMSDHEFDALFTK
                     DKPVIFAFHGYPSLIHQLIYRRSNHANFHVHGFAEQGTTTTPFDMVVLNELDRFHLAL
                     DVIDRLPQLGPRAAYFRQAIRDRLLDHKQYIERYGEDQPEIAGWRWDVSQPAAGRTNG
                     RPPTRPVATSTEGDNI"
     gene            2459..3637
                     /locus_tag="VFV99_23160"
     CDS             2459..3637
                     /locus_tag="VFV99_23160"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX5062297.1"
                     /translation="MRILLLNAGSSSLKCSLMESTDRTVVARATADWAGSVTRYERVG
                     PGDERVSEAVPLRGHGDAVRYALDDLATKAPTSERPFAVGHRIVHGGDLNTTVRITPA
                     VRSRISALADLAPLHNPPGLDTLATAEAVLPRVPHVAVFDTAFHATLPAAAYTYPLPQ
                     AWSTDWRIRRYGFHGLSHSYATARAAELLDRPPNELRLITCHLGAGCSATAVLHGRSI
                     DTTMGFTPLDGLMMATRCGSLDPGILTHVQLHHGMSARELEATLNQRSGLLGVSGISA
                     DMREVLAARRANHERARLAVDVYVHRVRQAIGALAVTLGGVDALVFTAGVGENSAEIR
                     MLVCQRLECLGLEIDTDANARCQPDADIARQDSRARILVIAAREDLAMLSEVVAAIET
                     "
     gene            3651..4724
                     /locus_tag="VFV99_23165"
     CDS             3651..4724
                     /locus_tag="VFV99_23165"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5062298.1"
                     /translation="MEALSPRVTNLGHTALALVSHGKGILAADETVSTLTKRFDALKI
                     HSTPDSRRDYREMLLTTPGIEQFIGGVIMHDETIHQNSSDGTPLVDLCDERGLLVGIK
                     VDAGAKMLAGSEQELVTEGLDGLRKRLQDYRRIGARFAKWRAVIVVGDALPTLGCVRG
                     NADALARYAALCQEQELVPIVEPEVLMDGPHTIERCEEVTSTVLHAVFDALFAHGVAL
                     EGIILKPNMVISGDECEQQADVATVAAATLRTLRRHAPAAVPGIAFLSGGQRDVLATR
                     HLDAMNRASGPKPWKLSFSYGRALQDAALITWRGRHDHVEAAQRAFYHRARCNSLSAR
                     GVYSEHEEQFAGERHIESPQPHR"
     gene            4743..6692
                     /gene="tal"
                     /locus_tag="VFV99_23170"
     CDS             4743..6692
                     /gene="tal"
                     /locus_tag="VFV99_23170"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5062299.1"
                     /translation="MQNGRSKLGTPISVVLTDVDGTLLTHDKILTERARSAVQRLHDK
                     GIMFTITSGRPPFGMRHLVDPLGLTLPMAAFNGGAIVTPDLSVLDVRGLPEYLVPALI
                     DMVLSHGLDVWLFRTRDWIVRDPKAPRVDRETFNIQTPPVVVSKFDDVLIDVVKIVGT
                     STNRASVVACEAAIEKAFGTQVFAARTNDMSLDITHPQANKGVVIERLGRLLKIPLER
                     IAALGDQANDLAMLERAGLSIAMGNAPDELKSKARIVTTSHADEGFANAIEQYILPRA
                     EPFGGPSVRPTAWLHRLGQSLWLDNITRDWLVNGTLARAIEELSLTGLTSNPTIYEHA
                     LKKSSAYDAAFAELARQGASSEDLFFELAIEDLKQAADMFRPIWDRSDGVDGWVSLEV
                     SPLLAKDPVGTLAAAKALFTKAGRPNLMIKIPGTREGLIAVEEAIFLGIPINVTLLFS
                     REHYLAAAEAFVRGIERRIEAGLKAEVASVASVFVSRWDNAVVGKVPEELRNTLGIAM
                     AQRTYKAYRSLLASPRFQRIYNAGARPQRLLWASTGTKDPKASDVLYVSALTAQFTVI
                     TMPEATLKAVADHGKIVTLMRADGGMCEQQLAKLKAAGVDLYGLAEQLQIEGTQSFEK
                     SWNQLMSFIATKAAPLQKPPPRATR"
     gene            complement(6711..6995)
                     /locus_tag="VFV99_23175"
     CDS             complement(6711..6995)
                     /locus_tag="VFV99_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062300.1"
                     /translation="MNRDVNVNGDYGHYHGGYYGGGWGYGVGAGVVAGTAAGLTAAAI
                     GSVAYSLPPSCATVYSYGSPYYHCGSTYYAPQYQGDDVTYVVVNPPEGTE"
     gene            complement(6992..>7320)
                     /locus_tag="VFV99_23180"
     CDS             complement(6992..>7320)
                     /locus_tag="VFV99_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062301.1"
                     /translation="RKQDKQELHADKPALHSGKLVLRNADKPALHSANPEPQVLRNAD
                     KRVSAKRERQAKAQRRVAKAQQRVRKVRVARKPVRELRPKATGKQISTRTSSTAIATS
                     ITSTPT"
BASE COUNT         1336 a         2428 c         2309 g         1247 t
ORIGIN      
        1 cttgcgaccg atggtccgct ctctgcaact gaactcgaca aactggacgc ctactggcgg
       61 gcgttgaatt atctgtcggt cggccaaatc tacctgctcg acaatccgct attgaaagaa
      121 acgcttaagc gcgagcacat caagccgcgt ctgctgggcc attggggtac gtcacctggc
      181 ctgaacatgc tctgtacgca tctcaatcgt gtcatcaaac gtgatgacct gaacatgatc
      241 tacatcatcg gcccgggtca cggcgggccg tcgctggtgg cgcatgccta cctggagggg
      301 acatacagcg agtactaccc gaatgtcagc caggacgccg aggggatgcg gcggctgttc
      361 aagcagttca gcttcccggg cggcattccg agtcacgtcg caccggagac tcccggatcg
      421 atccacgaag gtggcgagct tggttacgcg ctgtcacacg cgtacggcgc tgctttcgac
      481 aatcccgatc tgatcgtcgc gtgtgtggtc ggcgacggcg aagccgaaac cggtccactc
      541 gcgaccgcgt ggcacggcaa caagttcctc aacccgcaac gcgacggcgc cgtcctgccg
      601 atcctccacc tcaacgggta caagatcgcg aacccctgct acctcgctcg catcccgaaa
      661 gacgagctca cgaagctcat ggagggtctc gggtacaagc cgtacttcgt cgagggcgac
      721 gatcctgcgc tcgttcatcc gctgatggcg gagacgctcg agaagatcgt ggccgacatt
      781 cgcgacatct ggcgcgacgc gcgcgagcgc ggcaaggtca agcgcccgta ctggccgatg
      841 atcgtgctgc gcacgccgaa aggctggacg tgtccgcacg agatcgacgg caagctcacc
      901 gagggctact ggcgcagtca ccaggtcccg atgggcgata tggacaaggc cgagcacatc
      961 caggtgctcg agcgctggat gaagagctac cgtcccgacg agctcttcga cgcgaaaggg
     1021 cgcctgcgcc acgagctcgt cgtcggcgcg atgccccatg gcgacaagcg aatgagcgcg
     1081 aacccgcacg cgaacggcgg catgatcctg cgcgacctgc ggttgcccga cttccgcgac
     1141 tacgcgatcg aggtcacctc gccgggtggc gtgatgggcg agtcgacgcg gcgacaaggc
     1201 cgctacctgc gcgacgtgat gaagatgaac ctcgcgtcgc agaacctccg gctcttcagt
     1261 cccgacgaga acaactcgaa tcgctggggc gacgtgctcg aggtgacgaa tcgttgctac
     1321 atggcggaga tctaccccga ggacgatcac ctctcacccg acgggcgcgt gatggaggtg
     1381 ctgtccgagc accagtgcca gggttggctc gaaggttatc tgctcacggg acgccacggc
     1441 ttcatgagct cgtacgaggc gttcatccac atcgtcgatt cgatgttcaa ccagcacgcg
     1501 aagtggctga aggcctgcaa acacatcccg tggcggcggc cgatcgggtc gctcaactac
     1561 ttgctgagct cgcacgtctg gcgtcaggac cacaacgggt acagccatca agatccaggc
     1621 ttcctcgacc tcgtcgcgaa caaaggctcc gatctggtcc gcatctatct gccgccggat
     1681 gcgaacacgc tgctcgtcat taccgacgag atcatgcgca gccgggacat ggtcaacgtg
     1741 atcgtcgccg gcaagcagcc gcagccacag tggctgacga tggatcaagc gatcaagcat
     1801 tgttactcgg gcatcggcat ctgggactgg gcgagcaatg acttcaacga ccagcccgat
     1861 gtcgtgatgg cgtgctgcgg cgacgtgccg acgctcgaga cgctcgcggc ggtctcactc
     1921 gcgcgccgtc atctaccgaa tctcaagatc cgtgtcgtca acgtcgtcga cctgttgcgt
     1981 ctgcagtcca accaggaaca cccgcacggt atgtccgatc acgagttcga cgcgctgttc
     2041 acgaaagaca agcccgtcat ctttgcgttc catggctatc cgtcgctgat ccatcagctg
     2101 atctatcgcc gctcgaacca cgcgaacttc cacgtccacg gcttcgcgga gcaaggcacc
     2161 accacgacac cgttcgacat ggtcgtgctc aacgagctcg atcgcttcca cttggcgctc
     2221 gacgtgatcg atcgcctccc gcagctcgga ccgcgcgccg cgtacttccg gcaagcaatc
     2281 cgcgaccgcc tgctcgacca caagcagtac atcgagcgct acggcgaaga ccagccggag
     2341 atcgctggct ggcggtggga cgtgagccaa ccagccgcag gccgcacgaa cggacggccg
     2401 ccgacacgac cggtcgcgac gtcgacagaa ggcgacaaca tctaacgcgg agctcgccgt
     2461 gagaatcctg ctcctcaacg cgggctctag cagcttgaag tgctcgctga tggagtccac
     2521 ggatcgcacc gtggtcgcgc gcgccaccgc ggactgggca ggctcggtca cccggtacga
     2581 acgcgttggc cccggtgacg agcgcgtatc cgaggcagtg ccgctgcgtg gtcacggcga
     2641 tgccgtgcgc tacgcgctcg acgatctcgc gacgaaggcg ccgaccagcg agcgaccgtt
     2701 cgcagtcggc caccgaatcg tgcacggcgg cgatctcaac acgaccgtgc gcatcacgcc
     2761 ggccgtgcgt tctcggatca gcgcactcgc ggatctagca ccgctccaca atccacccgg
     2821 cctcgacacg ctcgccaccg ccgaagcggt gctgccacgt gtgccgcatg tggcggtgtt
     2881 cgacaccgcg ttccacgcga cgttgcccgc cgcggcgtac acgtatccat tgccacaggc
     2941 gtggtcgacc gactggcgga tccggcgcta cgggtttcac ggcctcagcc attcctatgc
     3001 aaccgcgcgc gcggcggagc tgctcgatcg accgccgaac gagctgcgac tcatcacctg
     3061 tcatctcggc gccggttgtt cggcgactgc cgtgctgcac gggcgatcga tcgacacgac
     3121 gatgggcttc acgccgctcg acggtttgat gatggcgacg cgctgcggct cgctcgaccc
     3181 cggaatcctc acgcacgtgc agctgcatca cggcatgtcg gcgcgagagc tcgaagcgac
     3241 gctcaaccag cgctccggtc tgctcggcgt atcgggaatc tcggccgaca tgcgcgaagt
     3301 gctcgcggca cggcgtgcga accacgaacg agctcgactc gcggtcgacg tctatgtcca
     3361 ccgtgtgcgg caagcgatcg gcgcgctggc cgtgacgctc ggcggcgtcg atgcgctcgt
     3421 gttcaccgct ggcgtcgggg agaactccgc ggagattcga atgttggtct gtcagcgtct
     3481 cgagtgcctc ggcctcgaga tcgacaccga cgcgaacgca cgctgccaac cagatgccga
     3541 catcgcgcgg caggattcgc gtgcgcgcat tctcgtgatc gccgcgcgcg aggacctcgc
     3601 gatgctctcg gaagtcgtcg ccgcgatcga gacatgaaac ggagtgagtc atggaagcac
     3661 tgagcccacg cgtaacaaac ctcggccaca ccgcgctcgc gctcgtctcc cacggcaagg
     3721 gcatcctcgc cgccgacgag accgtctcga cgctgacgaa gcgtttcgac gcgctgaaaa
     3781 tccactcgac accggacagc cggcgcgact accgcgagat gttgctaacg acacccggca
     3841 tcgagcagtt catcggcggc gtgatcatgc acgacgagac gattcaccag aacagcagcg
     3901 acggcacgcc gctcgtcgac ctgtgcgacg agcgcggctt gctcgtgggc atcaaggtcg
     3961 acgcaggcgc aaagatgctc gccggtagcg agcaggagct ggtcaccgag ggcctcgacg
     4021 gcctgcgcaa gcggctgcag gactaccggc gcatcggcgc tcgcttcgcg aaatggcgcg
     4081 ccgtgatcgt cgttggcgat gcgctgccga cactcggctg cgtacgcggc aatgccgatg
     4141 cgctcgcgcg ctacgcggcg ctatgtcagg agcaagagct cgtgccgatc gtcgagcccg
     4201 aggtgctgat ggacggaccg cacacgatcg agcgctgcga agaggtcacc tccaccgtgc
     4261 tgcatgcggt gttcgacgcg ctcttcgcac atggcgtcgc gctcgaggga atcatcctca
     4321 agccgaacat ggtgatctcg ggtgacgagt gcgaacagca agccgacgtc gcgaccgtgg
     4381 ccgcggcgac gttgcgcacg ctgcgccggc acgcaccggc ggcagttccc ggcatcgcat
     4441 tcttgtctgg cggccaacgc gacgtgctcg cgacgcgtca cctcgatgcg atgaatcgcg
     4501 cgagcggtcc caagccgtgg aaactgagct tctcctacgg ccgcgcgttg caagacgccg
     4561 cgttgatcac atggcgtggc cgccacgacc acgtcgaagc tgcgcaacgc gcgttctatc
     4621 accgcgcccg ctgcaacagc ctgtcggcgc gcggcgtgta cagcgagcac gaggagcaat
     4681 tcgccggcga gcgccacatc gaatcgccgc agccacatcg ctgacccaca gggagactcg
     4741 acatgcagaa cggacgttca aagctcggca cgccgatctc cgtggtgttg accgatgtcg
     4801 atggcacgct gctcacgcac gacaagatcc tgaccgagcg tgcgcgctct gctgtccagc
     4861 ggcttcacga caaaggcatc atgttcacga tcacgagcgg gcggccgccg tttggcatgc
     4921 gccacctcgt cgatccgctc gggctgacgc tgccaatggc ggcgttcaac ggcggcgcga
     4981 tcgtgacacc ggatctgtcg gtgctcgacg tgcgcggact ccccgagtac ctcgtgcccg
     5041 cgctcatcga catggtgctg tcgcacgggc tcgacgtgtg gctgttccgc acgcgcgact
     5101 ggatcgtgcg agacccgaag gcaccgcgtg tcgaccgcga gacgttcaac atccagacgc
     5161 cgccagtcgt cgtgtcgaag ttcgatgacg tgttgatcga tgtcgtcaag atcgtcggca
     5221 cgagtacgaa tcgcgcgagc gtcgtcgcgt gcgaggctgc gatcgagaag gcgttcggca
     5281 cgcaagtgtt tgcggcgcgc acgaacgaca tgtcgctcga catcacgcat ccacaagcga
     5341 acaagggcgt cgtgatcgag cgactcggcc gccttctcaa gatcccgctc gagcgcatcg
     5401 ccgcgctcgg cgatcaggcg aacgacctcg cgatgctcga gcgtgctggt ctcagcatcg
     5461 cgatgggcaa cgcgcccgac gagctcaaga gcaaagcgcg catcgtcacg acctcgcacg
     5521 ccgatgaagg cttcgcgaac gcgatcgagc aatacatcct gccgcgcgcc gaaccgttcg
     5581 gcggcccgag cgtgcgcccc accgcgtggc tgcatcggct cggtcagagc ttgtggctcg
     5641 acaacatcac gcgcgactgg ctcgtcaacg gcacgctcgc acgcgcgatc gaagagcttt
     5701 cgctcacggg gctcacctcg aacccgacga tctacgagca tgcgctcaag aagagctcgg
     5761 cgtacgacgc cgcgttcgcc gagctcgcgc gtcagggtgc atcgagcgaa gatctgttct
     5821 tcgagctcgc gatcgaggat ctcaagcaag ccgcggatat gttccgtccg atctgggatc
     5881 gcagcgacgg cgtcgacggc tgggtttcac tcgaggtgtc accgctgctc gcgaaggatc
     5941 ctgtcggcac gctcgcggct gcgaaggcgc tgttcacgaa ggctggccgc cccaatctca
     6001 tgatcaagat ccccggcacg cgcgagggtc tgatcgccgt cgaggaagca atctttctag
     6061 gcatcccgat caacgtcacg ctgctgttct cgcgcgagca ctacctggct gccgcggaag
     6121 cgttcgtgcg cggcatcgag cgccggatcg aggccgggct caaggcagaa gtcgcatccg
     6181 tcgcgtcggt attcgtcagt cgctgggaca atgcggtcgt cggcaaagtc cccgaggagc
     6241 tgcgcaacac gctcggcatc gcgatggcac aacgcacgta caaggcgtat cgcagcttgc
     6301 ttgcgtcgcc gcgcttccag cggatctaca acgccggcgc acgcccgcag cggctgttgt
     6361 gggcgagtac gggcacgaag gatccgaaag cgtccgacgt tctttacgtc agcgcgctga
     6421 ccgcgcagtt cacggtcatc acgatgcccg aggccacgct gaaggctgtc gccgatcacg
     6481 gcaaaatcgt gacgctgatg cgcgccgacg gcggcatgtg cgagcagcaa ctcgccaagc
     6541 tcaaggcagc cggcgtcgat ctctacggac tcgccgaaca gctacagatc gagggcacgc
     6601 agtcgttcga gaaatcgtgg aaccagctga tgtcgttcat cgcgacaaag gctgcaccgc
     6661 tgcaaaagcc gccaccgcgc gcgactcgct agtggcgcgg aggtagtgcc ctactctgtt
     6721 ccttccggcg ggttgacgac gacgtacgtg acgtcgtcac cctggtactg cggcgcgtag
     6781 taggtgctac cgcagtggta atacggcgag ccgtagctgt agaccgtggc gcagctcggc
     6841 ggcagcgagt acgcgaccga accgatcgcc gcagcggtca ggccggctgc ggtgccggcc
     6901 acgacacccg cgccgacgcc atagccccaa ccaccgccat agtaaccacc gtggtagtgg
     6961 ccatagtcgc cgttgacgtt gacgtcacga ttcacgttgg cgttgacgtt attgatgttg
     7021 cgattgctgt tgagctggtt ctggttgaga tttgcttgcc ggttgccttg ggccgcagtt
     7081 ccctgacggg cttgcgtgcc acccgcacct tgcgtacgcg ttgctgtgcc ttggccacgc
     7141 gccgctgcgc cttggcctgt cgttcccgct tggcgctgac ccgcttgtcc gcgttgcgca
     7201 gtacctgcgg ttccgggttg gcgctgtgca gtgccggctt gtccgcgttg cgcagtacca
     7261 gcttgccgct gtgcagtgcc ggcttgtccg cgtgcagctc ctgtttgtcc tgcttgcggg
//
LOCUS       DASUFX010000577        14344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_233787,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000577 DASUFX010000000
VERSION     DASUFX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233787"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..990
                     /locus_tag="VFV99_23185"
     CDS             211..990
                     /locus_tag="VFV99_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062302.1"
                     /translation="MRVDSSIITSLSELRAIEQQRIADERAAVDRERAAQVEAKRAAE
                     QAIRDAEEKKLREEREALMRIEQARVDAEREARMRVESAEAAERMRLQAALEQQRMQE
                     ESELRRAEIAKKRPTWMLAVTGLALVATLGFGYFAYERNEARQEAERAKIAADNDKRE
                     AQDDAAQSRAELDKLTLQMEELDKQVEQAQKVLLAAQTKADREAAGRAIAEANRQRAE
                     IKKQQDAIKLKEWKKKRNEKFVVSEECKRNPLAPGCLGDSK"
     gene            1083..3419
                     /locus_tag="VFV99_23190"
     CDS             1083..3419
                     /locus_tag="VFV99_23190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5062303.1"
                     /translation="MRGPIVVAIAISIFVPNCQCGKDNGATTPDAAFVCNPSCEGDLV
                     CRYDVCVPPPTPCTTNAECPGDEYCDVSRMECLPWGVGPGGGSDPECKRDPVPGVFFP
                     GAQCDWTAPAANDFPGHVNVLATPVVATFYSQGELASPSIVFTSYNGADGSADACQSA
                     TPATYYGVVRIIDGRTCMPQATISSPTVVASASLAVADLGGADATPEIVAARSDGGLV
                     AWTLGPTGWGVLWQTTSTFADTNCDWAGPSIHDLDDDGIPEVLFYGNVYDGRTGAALD
                     ETLGTTVDNISDGYIPVAADIDGDGNVELITGKMIYVWNKATKKWDTEYPLTNGSGNG
                     FVAVADFGTYPANGADDRGTLDGIAEVALIYNSVATVLTRDNRVVFTASLQGASPGRG
                     GAPTIADFDGDGRVEFASAGAITYNVFDPDCIGATPDPQYCKQRIAPGVLWTQPSQDG
                     SSNMTGSSVFDFDGDGRAEVVYGDECFTRVYDGVTGTVLYSRYRTSCTWYENPVIADV
                     DADFNAEIVSTSNVNCPTVTCPTVDPIFDGVRCLDDSDCPSATKCGRDQASDPLGRCR
                     CSIDADCGGDGFVCLPSIAPSTAGNVCRASHPGTRLTGIRVLADGIDRWVNTRTVWNQ
                     HAYSVTNIDEYGKVPRTSQWAANWKQPGLNNYRQNSPGDGQTAGAIPDLTVKQAKVTC
                     ETSGPVITVEVCNRGTEPVAPGLPVAVYSDGPPKELRCQTTTMQRINPGFCATVSCVW
                     TGGGGAGTVVVDDRGDATGIASECREDNNVFAVSVTCP"
     gene            3508..4008
                     /locus_tag="VFV99_23195"
     CDS             3508..4008
                     /locus_tag="VFV99_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062304.1"
                     /translation="MRGLVVVVAVLLLPSVSARADDDLRVTAEPSPPSKHLVYVELLG
                     KGGLYGVGYEYAITDWLGFGGAASYADIRDEQVITVSPYLHFTVLASERHALFTEVGG
                     ILAHSHVPSPVMNWDGVSDTGGGGYLSVGWEYRRRHVVLRTSGSVVAGEGGVAPMVGF
                     AIGARP"
     gene            4005..4904
                     /locus_tag="VFV99_23200"
     CDS             4005..4904
                     /locus_tag="VFV99_23200"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5062305.1"
                     /translation="MNVGGGRAAVALLALAACTDIADDGTPWQDAAEVVGTEVGPAPT
                     LRAASGCTLRIASWNVHFLPDPQDLLAHLRASREIANADVILLQETSTWPGDERSRTA
                     GLADQLAMTWAHTPVVELPNGVYQGNGILSRFPLERVAVKRLPYIARPAFDQARTALA
                     ADIVVGDQRVRVVSVHLDVRVQITDAIRQLDPAVTDLDERAVVGGDFNTAPWQWVDGV
                     VPLTSTEAVVGMKQAAILDDYMASRRYQSAISPDTNTFTLPGFGMRLDNVYPRAIPLL
                     DAGVEHVGGSDHWPIYADVDLCN"
     gene            complement(4901..5518)
                     /locus_tag="VFV99_23205"
     CDS             complement(4901..5518)
                     /locus_tag="VFV99_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062306.1"
                     /translation="MPEQKPKKSKREIWFKFGRFITGVTIACFFLPFFGVSCEGMEFV
                     HFSGADMVGGCKPGGMAVEAEEQKQEKGMGGGDMGGDIKVENVDREPLAIVALGLALL
                     VAGLSWVRSRGAMIGALALSLACIGALAGLYMKVGGDLDKKISAELSSKNAGSQMMKE
                     TKVDAGSRFGLWLTVGGLLGVAVLTGMALKEKDGAAFTPSTPPPA"
     gene            5598..6158
                     /locus_tag="VFV99_23210"
     CDS             5598..6158
                     /locus_tag="VFV99_23210"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5062307.1"
                     /translation="MKALLCACLFVVACKGESSKSSSGESKPTEQATKVERSRPRPSL
                     PGSPNTPDDQRRRPRLPDEAEPRDWSDPAVREEMRAKMEERRKAREAALDTNHDGVVS
                     PEEHQARLKPMIERFDQNGDGKLTPEELASSDRRMGFDDPAALDTDHNGEISLSELDA
                     AVTQRREEMRARWRGRNGGSAQGVGE"
     gene            complement(6177..6956)
                     /locus_tag="VFV99_23215"
     CDS             complement(6177..6956)
                     /locus_tag="VFV99_23215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5062308.1"
                     /translation="MRTLVLIAALLAAGCVKKSTYQKAVDENNRLKTELADTQKKLAE
                     TEQTLSEKIKELQAQLGVLDETTKTKEAELGKVRGEKEATLAELAELRRQKEAAEKRI
                     SAYKALQDKFRALVDTGKLQVVFRNGQMTLKLPSGILFPSGSADLSKGGQTAIADVVK
                     VLMEFKDRRFIVAGHTDNQPIKTAQFPNNWYLSTARANSVVQYMIKEGFPAKSLAASG
                     YGEFDPVAANDTDKNREQNRRIEIILVPNLEELPSLTGNQP"
     gene            6989..8839
                     /locus_tag="VFV99_23220"
     CDS             6989..8839
                     /locus_tag="VFV99_23220"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HEX5062309.1"
                     /translation="MKPEATEDPEAVWLRDVYQPNATNLTVRAVIGGMLIGCVMCVSN
                     IYVFFKTGWSMGVTITACILAFSVFGLVRSIGGKRVRELGMLENNALTTVASGAGYMT
                     GGGNMAAWGALLMVTAVRPPQVPMVLWFAVIASLGVFAAIPIKRQLINKEGLAFPTGT
                     ATAETLRSIHGENLVPAGEPVPRAQVGRLPAETKTPSAAKALALAAAFGAAVGAIRDI
                     AKWVKPAYSLPYTISGHPLVQWTLALKTEVILLGAGALMSFRTGWSVLLGGLLTYGVM
                     GPSLLEQHLIKGVSYKAIVEWTVWPGAAILVGAGLTSFALDYKSLGRAFTGLSQIVRG
                     RRERAEQGISAVEAPEWWFPAGFMALTPIIVFLMAYLFQIPIWAAILAVPLAVLMGFI
                     AARVTGETDVTPTKALGPVTQMMYGAVTPGNLSGNIMAANVTGGVGLHAADLLTTLKT
                     GWLLGAKPRHQLYAQLFGVLAGAFIVVPAFNLIIPDPAMLGTDDWPAPSCLIWAGVSK
                     AFAGGIDNLGHEARVAIAVGLSLGVGLALLEKLAPKRMRAFVPSPYGLGIAMVVPGSN
                     CISMFLGAAIAEVFRRRKRESIIVPVASGLIAGESLMGVLVALLVAGGVL"
     gene            8856..10640
                     /locus_tag="VFV99_23225"
     CDS             8856..10640
                     /locus_tag="VFV99_23225"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5062310.1"
                     /translation="MRRLIALLVTLGACKHARAPEGPDPHSFAQPQQVAVRHLALDLA
                     VSFEQRTLAGTATLSLTRTDRKAPLVLDGDGLAIEAVADCAGKPLTFTVGTHTSMGEP
                     IRVELGTADCVAIRYHTKPDASALLWVEPSGTAGGKQPMLFTQSQATRARSWIPLQDS
                     PGVRFTYEATIHPPAGLWALMSTPNPQASPADGVWRFRQEHPIPSYLMALAVGDFAFR
                     STGPRSGVYAEPSVVEGAAWEFAEVEAMIDAAEKLYGPYRWGRYDMLVLPPSFPFGGM
                     ENPNLTFLTPTAISGDRALVSLIAHELAHSWSGNLATNSTWNDTWLNEGFTTYVEYRI
                     MEQLRGSEFSDVLWFLGRKEVERAVAKYGSGPATHLAHHYGRDTDAEEIPSGLAYDKG
                     ALFLRTLELAYGRAAFDTWLRGWFDRHAFQAVDSKQFSAEIKPLGSKVDIATWVDAGG
                     LPADAAPAPSAHAAAIEQLATSGGDVDASSWNTMDWVVYLGALPETVPAARLLALDAR
                     YHLTGTSNAEIAMHWLPLVVRADVRDAAPAVEAFLSKVGRVRMVRPLYTAMMAGTDFW
                     HALAKSTFERAKPKYHPITRGAITDIVK"
     gene            complement(10641..12272)
                     /locus_tag="VFV99_23230"
     CDS             complement(10641..12272)
                     /locus_tag="VFV99_23230"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5062311.1"
                     /translation="MRAASIAMIVLASCSFTHGREQEVDAGVDAPPMTPNVYFTSVTS
                     TVTQVRPGKYGFEVTAVLRNDLATEITDVGARLTFGGHDKEIRHRDEDRREGVFDLQP
                     TTLAPGTEATYRFVVDALPWLTASDLQVNAAAVFTTSGSKLSATPAATPLTLSYGGVN
                     GPIVVTTEKDESNTGSKLSFREALAQAMTAPGPDIITFDPSVFTPGTVVDLDDNLGEL
                     PTITSDVVIDGTSADVTLALAASWESPEGRYGLRIVNGTVAVAGITFRNFAFNYRNED
                     LTTNNCGISNAQLEGGAMRVDGGTLILERNRFEDPDVAERNCYAASVRLHGGTAHHIV
                     SNTWTDQVMDSIYVAAKVTEISDNVMISPNHTDRDDEGIYIASQGGGDLWIVGNLIVD
                     QEYSGIYAGGSDNGRLWIFNNTFVRNGRSSLAAIRRSGPRVIVLRNNLYIANNPSALQ
                     ADNNGTGFDIAYETVSSNSLCSGTCDSAMIDMPSVLMPADPGVANIAGTTPDDFTPTA
                     TSPLMGTATPILDRNGGAPNHFMGDGQERGCIEIP"
     gene            complement(12341..13450)
                     /locus_tag="VFV99_23235"
     CDS             complement(12341..13450)
                     /locus_tag="VFV99_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062312.1"
                     /translation="MTDPLRPALLTPARPHMRRMLLTALGLTAIGAAVSLGSDTHETR
                     RVTFRMPVLPKVEPVVTPIVEKPVAIAQPVRSMELGLVFSAGGATYMRLTEVGDGRDV
                     AMPKHGKLRLHSADGVESAIATVRDSDVPIGYRRWQDRDVIVDNNCRTTVTGFAVVAR
                     LTGDTGYAGIEASKWTAKTVFDSGAKVLAAKLADCDGTFARDAALPAVVVPDSIEDET
                     LASRARAALIASEAAHDTQSEYTKFYAEYDADGEAHGNWWDEKYVNFDTRVVRHPTTG
                     VTWVSIHGSLDHSCGDPTVNVWGLFRVETDGTLAPVQLRKLDDLWEIDQLVDIDNDGE
                     LELIGKPWLGLDTVVVRANSEELSRLELPFFGCPC"
     gene            13619..>14344
                     /locus_tag="VFV99_23240"
     CDS             13619..>14344
                     /locus_tag="VFV99_23240"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062313.1"
                     /translation="MRVLTTLLLLTGLAHADVGVVTTGEPMMQAPVLAQVQGWLQQHQ
                     YGLVAVPLGDAANSFMDCFVMEDMGCAQKSFEKDSKASNLIYVRADLMAGQARDFNLI
                     AYWFVKGHEPVLQKRECHGCDDAKLGPIVGSLMTDLEKGISSTKGHLKITSVLGGVSV
                     TIDNVSLGAAPLEHDLDPGPHEISFLHRGKVLETKPLKIEPGATVEMAAPTLTIEAPP
                     PAKQRSRALPVSLAVVGAALAVTG"
BASE COUNT         2392 a         4669 c         4794 g         2489 t
ORIGIN      
        1 gccctgatga tcgttgccgt cgagaccgtc ggcctcctcg cgtatgccgg cgcgccggag
       61 ccgcaggcgt cggcgtcggt aggcaacgcg cagagttggt ccgacgcgca ggtccgcccg
      121 atcaaagttc gctgccgccg ctgaccgcgc gctacccgta gcgcctcgct ggtgcctgca
      181 atcggctcgt cgagccgctg tcttctcggc atgcgggtcg actcatccat catcacgtca
      241 ctgtccgagc tgcgcgccat cgaacagcaa cgcatcgccg acgagcgcgc ggccgtcgat
      301 cgagagcgcg ctgcgcaggt cgaagcgaag cgcgccgccg agcaggcgat tcgcgacgcc
      361 gaagagaaga agctccggga ggagcgcgag gcgctgatgc gcatcgagca ggcgcgtgtc
      421 gatgccgaac gcgaagcgcg catgcgcgtc gagtcggcag aggcggcgga gcgcatgcga
      481 ctgcaggcgg cgctcgagca gcaacggatg caggaagaga gcgagctgcg ccgcgcggag
      541 atcgcgaaga agcggccgac gtggatgctc gcggtgacgg gcctcgcgct ggtcgcgacg
      601 ctcggcttcg ggtacttcgc gtacgagcgc aacgaggcgc gccaagaagc tgagcgggcc
      661 aagattgccg ccgataacga caagcgagaa gcccaagacg acgccgccca gtcgcgcgcg
      721 gagctcgaca agctcacgct tcagatggaa gagctcgaca agcaggtgga gcaggcgcaa
      781 aaagtgctgc tcgccgcgca gaccaaggca gaccgcgagg ctgcgggtcg cgccatcgcc
      841 gaagcgaatc gtcagcgcgc tgagatcaag aagcagcaag atgcgatcaa gctgaaggaa
      901 tggaagaaga agcgaaacga gaagttcgtc gttagcgagg aatgcaaacg gaacccactc
      961 gcgcctggct gtctgggcga ctcgaagtag ccgccatcga ccgaccgagt ggatggggac
     1021 agttgaccgg agacaactga cccgacgacg acgcgcgcag cgacgtgtag attggtttgc
     1081 agatgcgtgg gcccatcgtc gtcgcgattg cgatctcgat cttcgttccg aactgccaat
     1141 gcggaaaaga caacggcgcg acgacaccgg acgcggcgtt cgtctgcaat ccgtcgtgcg
     1201 aaggcgacct cgtgtgtcgt tacgacgtgt gcgttccacc gccgacgccg tgcacgacga
     1261 acgcagagtg ccccggcgat gagtactgtg acgtctcgcg gatggagtgc ttgccgtggg
     1321 gcgtcggtcc cggcggcggc agcgatcccg agtgcaagcg cgatccggtt cctggcgtgt
     1381 tcttcccagg tgcgcagtgc gactggaccg cgccggccgc gaatgatttt ccggggcacg
     1441 tgaacgtgct cgcgacgccc gtcgtcgcga cgttctattc gcaaggcgag ctcgcgtcgc
     1501 cgtcgatcgt gttcacgtcc tacaacggcg ccgacggtag cgcagatgcg tgtcagagcg
     1561 cgacaccagc gacgtactac ggcgtcgtgc gcatcatcga tggccgcacg tgcatgccgc
     1621 aggcgaccat ctcgtcgcca accgtcgtcg cgtccgcgtc gctcgcggta gccgacctcg
     1681 gtggcgccga tgcaacgccc gagatcgtcg cggcgcgcag cgacggcggg ctcgtggcgt
     1741 ggacgctcgg gccgaccggg tggggcgtgc tgtggcagac cacctcgacg tttgcggata
     1801 ccaattgcga ctgggccggt ccgtcgatcc acgacctcga cgacgacggc atccccgagg
     1861 tgctgttcta cggcaacgtg tatgacggtc gtacgggcgc ggcgctcgac gagaccctcg
     1921 gcacgaccgt cgacaatatc tcggatggct acattccggt cgcggcggac atcgacggcg
     1981 acggcaacgt cgagctcatc actggcaaga tgatctacgt ctggaacaag gcgacgaaga
     2041 aatgggacac cgagtacccg ctcacgaacg gcagcggtaa tggcttcgtc gcggtcgccg
     2101 acttcggcac gtacccggcg aacggcgctg acgaccgcgg cacgctcgac ggcatcgccg
     2161 aggtcgcgct gatctacaac agcgtcgcga ccgtgctcac gcgtgacaat cgcgtcgtgt
     2221 tcactgcgtc gctgcagggc gcgtcgcccg gacgcggcgg cgcaccgacg atcgccgact
     2281 tcgacggtga cggccgcgtc gagttcgcga gcgctggcgc gatcacgtac aacgtgttcg
     2341 atccggactg catcggtgcg acgcccgatc cgcagtactg caagcagcga atcgcgccgg
     2401 gtgtgctgtg gacgcagcca tcgcaagacg gctcgtcgaa catgaccggt tcgtcggtgt
     2461 tcgacttcga cggcgacggt cgcgccgagg tcgtctacgg cgacgagtgc ttcacgcgcg
     2521 tgtacgacgg ggtgacgggc acggtgctgt actcgcggta tcgcacgtcg tgcacctggt
     2581 acgagaaccc ggtgatcgcc gacgtcgacg ccgatttcaa cgccgagatc gtgagcacgt
     2641 cgaacgtcaa ctgcccgacg gtgacgtgcc cgacggtgga cccgatcttc gacggcgtgc
     2701 ggtgcctcga tgattcagat tgcccgagtg ccacgaagtg cgggcgcgac caagcgagcg
     2761 atccgctcgg ccgctgccgc tgctcgatcg acgccgactg cggcggcgat ggcttcgtgt
     2821 gcttgccgtc gatcgcgcca tcgacggcgg gcaacgtgtg ccgcgcgtcg catccgggca
     2881 cgcgccttac cggcattcgc gtgctcgccg atggcatcga ccgctgggtg aacacgcgga
     2941 cggtgtggaa ccagcacgcg tacagcgtca cgaacatcga cgagtacggc aaggtgccgc
     3001 gcacgagtca gtgggcagcc aactggaaac aacccgggct caacaactac cggcagaact
     3061 cgccgggcga cggccagacc gccggcgcga ttccagacct cacggtgaag caggccaagg
     3121 tcacgtgcga gacaagcggg ccggtgatca ccgtcgaggt ttgcaaccgc ggcaccgagc
     3181 cggtcgcgcc tggtctgccg gtcgctgtct actccgacgg tccgccgaag gagctgcgct
     3241 gccagacgac gacgatgcag cgaatcaatc cgggattctg cgcgaccgta tcgtgcgtgt
     3301 ggactggcgg tggcggggcg gggacggtcg tcgtcgacga tcgtggagac gcgaccggca
     3361 tcgcgtcgga atgtcgcgag gacaacaacg tctttgcggt cagcgtgacc tgcccctgag
     3421 agtttcaccg acgcgaaaag tcgggcacga ggcggcaaca aactcgcggt gagcaaccgc
     3481 ccgcgctgcg ttacgctctc ttcctcgatg cgggggctcg tcgtcgtcgt cgctgtgctt
     3541 ctgttgccgt ccgtcagcgc gcgcgccgac gacgaccttc gcgtcactgc cgagccatcg
     3601 ccgccgagca agcatctcgt gtacgtcgag ctgctcggca agggcggcct ctacggcgtc
     3661 ggctacgagt acgcgatcac ggactggctt ggcttcggtg gtgccgcctc gtacgccgac
     3721 attcgcgacg agcaggtcat caccgtctcg ccctacctgc acttcaccgt gctcgcgagc
     3781 gagcgccatg cattgttcac cgaagtcggc ggcatcctcg cgcactcgca cgtgccatcg
     3841 ccggtgatga actgggacgg cgtcagcgac acaggcggtg gcggctatct ctcggtcggt
     3901 tgggagtatc gacgcaggca cgtcgtgctg cgcacgagcg gctcggtcgt ggcaggcgag
     3961 ggcggcgtcg cgccgatggt cggattcgcg atcggagcgc gtccttgaac gtaggcggcg
     4021 ggcgcgctgc ggtggcgttg ctcgcgctcg ccgcgtgtac cgacatcgcc gacgacggga
     4081 cgccgtggca ggacgccgcc gaggttgtcg ggaccgaagt cgggccagcg ccgacgctgc
     4141 gcgcggcgag cggttgcacg ctacgcatcg cgtcctggaa cgtgcacttc ctccccgatc
     4201 cgcaggattt gctcgcgcac ctgcgcgcgt cacgcgagat cgcgaacgcc gacgtgatcc
     4261 tgttgcaaga gacgtcgacg tggccaggtg acgaacgatc gcgcaccgct gggctcgccg
     4321 atcagctcgc gatgacgtgg gcgcatacgc cggtcgtcga gctcccgaat ggcgtgtatc
     4381 agggcaacgg catcctgagc cgctttccgc tcgagcgcgt ggcggtgaag cgtttgccgt
     4441 atatcgcgcg gccggcgttc gaccaggcgc gtactgcgct cgcggcggat atcgtggtcg
     4501 gcgatcagcg cgtgcgcgtc gtcagcgtgc acctcgacgt tcgcgtccag atcaccgatg
     4561 cgatccgcca gctcgatccg gcggtgaccg acctcgacga gcgcgctgtc gtcggtggcg
     4621 acttcaacac ggcgccctgg cagtgggtcg atggcgtcgt accgctgacg agcaccgaag
     4681 cggtcgtcgg catgaagcag gccgcgatcc tcgacgacta catggcgtcg cgcagatatc
     4741 agagcgcgat ctcgccggac acgaacacgt tcacgctacc cggcttcggc atgcggctcg
     4801 acaacgtgta tccgcgcgcg ataccgctgc tcgatgccgg cgtcgagcat gtcggcggct
     4861 cggaccactg gccgatctac gccgacgtcg acctctgcaa ctaagcaggc ggcggtgtgc
     4921 tcggcgtgaa tgccgcgccg tctttttcct tgagcgccat gcccgtcagc accgcgacgc
     4981 cgaggaggcc accgacggtg agccacaaac cgaatctgga tccagcgtcg actttggtct
     5041 ccttcatcat ctgactaccg gcgttcttac tactgagctc ggcactgatc tttttgtcga
     5101 ggtcaccgcc gaccttcatg tagaggccgg cgagcgcgcc gatacatgcg agcgacagcg
     5161 cgagcgcgcc gatcatcgcg ccgcggctgc gcacccacga caggccggcg acgagcaacg
     5221 cgagtccgag tgcgacgatt gcgagtggtt cgcgatcgac gttctcgacc ttgatgtcgc
     5281 cccccatgtc accgccgccc atgcccttct cttgcttctg ctcctccgcc tcgacggcca
     5341 tgccgccggg cttgcagcca ccgaccatgt cggcgcccga gaagtggacg aactccatgc
     5401 cttcgcagga cacgccgaag aacggcagga agaaacacgc gatcgtgacg cccgtgatga
     5461 aacgtccgaa cttgaaccaa atctctcgtt tggacttctt cggcttttgt tccggcatat
     5521 tgcgcgagcg tatcaacggt cagtaaagaa gtgtgtaacg acaagccaag accgccctgg
     5581 attgccatga tggttgtgtg aaggctctcc tgtgtgcctg cctcttcgtg gtcgcctgca
     5641 agggcgaaag ctccaagtcc tcgtcgggtg agagcaagcc gaccgagcaa gcgacgaagg
     5701 tcgagcgcag ccggccacgt ccctcgctcc cgggctctcc gaatacgccc gatgatcagc
     5761 gccgccggcc gcggttgccg gacgaggcgg agccgcgcga ctggagcgat ccagccgtgc
     5821 gcgaggagat gcgcgcgaag atggaggagc gtcgcaaggc gcgcgaggcg gcgctcgata
     5881 cgaaccacga cggcgtggtt agccccgagg aacaccaggc tcgactcaag ccgatgatcg
     5941 agcgcttcga tcagaatggc gatggcaagc tgacgcccga ggagctcgcc agctcggacc
     6001 gccgcatggg cttcgacgat cctgctgcgc tcgacaccga tcacaacggc gagatttcgc
     6061 tctccgagct cgacgccgcg gtcacgcagc gtcgcgaaga gatgcgcgcg cgttggcgtg
     6121 gccgcaatgg cggcagtgcg cagggcgtcg gcgagtaggc tatcgcgcgc cgagactcag
     6181 ggttggttgc cggtcaggct cggcagctct tcgagattcg gcaccaagat gatctcgatg
     6241 cgacggttct gctcgcggtt cttgtcggtg tcgttcgccg ccaccggatc gaactcgccg
     6301 tagcccgatg ccgcgaggct cttcgccgga aagccttcct tgatcatgta ctgcacgacg
     6361 ctgttcgcgc gcgccgtcga caggtaccag ttgttcggga actgggcggt cttgatcggt
     6421 tggttgtcgg tatgacccgc gacgatgaag cggcggtcct tgaactccat caggaccttc
     6481 acgacgtcgg cgatcgcggt ctggccgccc ttcgacagat ccgccgagcc gctcgggaac
     6541 aagattcccg acggcagctt caacgtcatc tggccgttgc ggaatacgac ctgcaacttg
     6601 ccggtgtcga cgagagcacg gaacttgtcc tgcagcgcct tgtacgcgga gatgcgcttc
     6661 tcggctgctt ccttctggcg gcgcagctcg gccagctccg cgagcgtcgc ttccttctcg
     6721 ccacgtactt tgccgagctc cgcttccttt gtcttcgtcg tctcgtcgag gacgccgagc
     6781 tgcgcctgca gctccttgat cttctccgac agcgtctgct cggtctcggc gagcttcttc
     6841 tgagtgtcgg cgagctcggt tttgagccgg ttgttctcgt cgacagcctt ctgataggtc
     6901 gactttttta cgcagcccgc agcgagcagc gcggcgatca gtacgagtgt acgcatcacg
     6961 ctggacgata taggatgcgc gacggtcgat gaagcccgaa gcaacggaag atcccgaagc
     7021 cgtatggctt cgtgacgtct atcagccgaa cgcgacgaac ctgaccgttc gcgccgtgat
     7081 cggcgggatg ttgatcggct gtgtcatgtg cgtgtcgaac atctacgtct tcttcaagac
     7141 gggctggagc atgggcgtca cgatcacggc gtgcatcctc gcgttctcgg tgttcgggct
     7201 ggtgcggtcg atcggcggca agcgcgttcg cgagctcggc atgctcgaga acaacgcgct
     7261 cacgaccgtc gcgtcggggg ccggctacat gaccggcggt ggcaacatgg ccgcgtgggg
     7321 cgcgctactg atggtcacgg ccgtgcgccc accgcaggta ccgatggtgc tgtggttcgc
     7381 ggtcatcgcg tcgctcggcg tgttcgcggc gattccgatc aagcgccagc tgatcaacaa
     7441 agagggcctc gcgtttccga ccggcacggc gactgccgag acgctgcggt cgattcacgg
     7501 cgagaacctg gtgccagcgg gcgagccggt gccgcgtgca caggtgggcc gcctcccagc
     7561 agaaaccaaa acgccgagcg ctgccaaggc actcgcgctc gctgccgcgt tcggcgcagc
     7621 cgtcggtgcg attcgcgaca tcgcaaagtg ggtcaagccg gcctacagtc tgccgtacac
     7681 gatcagtggc catccactcg tgcaatggac actcgcgctg aagacggaag tcattctgct
     7741 cggcgccggc gcgctgatga gcttccgcac cggctggtct gtgctgctcg gcggcttgct
     7801 cacctacggc gtgatggggc cctcgctgct cgagcagcat ctgatcaagg gcgtcagcta
     7861 caaggcgatc gtcgagtgga cggtgtggcc tggtgccgcg atcctcgtcg gtgcgggcct
     7921 cacgtcgttc gcgctcgact acaagagcct tggccgcgcg ttcaccggcc tcagtcagat
     7981 cgtacgtggg cgtcgcgagc gcgccgagca gggcatctcc gcggtcgagg cgccggagtg
     8041 gtggttcccc gccggcttca tggcgctcac gccgatcatc gtgttcctga tggcgtacct
     8101 gttccagatt ccgatctggg ctgcgatcct cgcggtgccg ctcgcggtgc tgatgggctt
     8161 catcgcggcg cgcgtcaccg gcgagaccga cgtcacgccg acgaaggcgc tcggcccggt
     8221 cacccagatg atgtacggcg cggtcacgcc cgggaatctg tcgggcaaca tcatggcggc
     8281 caacgtcacg ggcggtgtcg gtttgcatgc tgccgatctg ctgacgacac tcaagaccgg
     8341 ctggctgctc ggcgccaagc cgcgtcacca gctctacgcg cagctgttcg gtgtgctcgc
     8401 cggcgcgttc atcgtggtgc cggcgttcaa cctgatcatt cccgacccgg cgatgctcgg
     8461 caccgacgac tggccggcac cgtcatgcct gatctgggcc ggcgtgtcga aggcgtttgc
     8521 cgggggcatc gataacctcg gccacgaggc gcgcgtcgcg atcgcagtcg ggctcagcct
     8581 cggcgtcggc ctcgcgctac tcgagaagct cgcaccgaag cgcatgcggg cgttcgtgcc
     8641 atcgccgtac ggcctcggca tcgcgatggt ggtgccgggc agcaactgca tctcgatgtt
     8701 cctcggcgct gcgatcgcag aggtgttccg ccgccgcaag cgcgagtcga tcatcgtgcc
     8761 ggtcgcgtcg gggcttatcg caggcgagag tctgatgggc gtcctggtgg cactccttgt
     8821 cgcgggcggt gtgttgtgac actacgtgcc gtgccatgcg tcggctgatc gcgctcctcg
     8881 tcacgctcgg cgcgtgcaag cacgcacgtg caccggaagg gcctgatccg cattccttcg
     8941 cgcagccgca acaggtcgcc gtccgccacc tcgcgctcga tctcgcggtc agcttcgagc
     9001 agcgcacgct cgccggcact gcgacgctgt cgctcacccg caccgatcgc aaggcgccac
     9061 tcgtgctcga cggcgatggc ctcgccatcg aagccgtggc cgattgcgca ggcaagccgc
     9121 tgacgttcac cgtcggcacg cacacgtcga tgggcgagcc gatccgcgtc gagctcggga
     9181 cagccgactg cgtcgcgatt cgctatcaca cgaaacccga tgcgagcgcg ctgctctggg
     9241 tcgaaccgag cggcaccgcg ggcggcaagc agccgatgct gttcacgcag tcgcaggcga
     9301 cgcgcgcgcg cagctggatc ccgctgcaag actcgcccgg cgtacgcttc acgtacgagg
     9361 cgacgatcca tccaccggct gggctgtggg cgctgatgag cacgccgaac ccgcaggcat
     9421 cgcccgccga tggcgtgtgg cgcttccgac aggagcaccc gatcccgtcg tacctgatgg
     9481 cactcgcggt tggtgatttc gcgtttcgct ccaccggacc gcgcagtggc gtgtacgccg
     9541 aaccgtcggt ggtcgagggt gcggcgtggg agtttgccga agtcgaagcg atgatcgacg
     9601 ccgccgagaa gctgtacggc ccgtatcgct gggggcgcta cgacatgctc gtgctgcctc
     9661 cgagcttccc gttcggtggc atggagaatc cgaacctgac gttcctcacg ccgaccgcga
     9721 tcagcggcga tcgtgcgctg gtctcgctga tcgcgcacga gctcgcgcac tcgtggtcgg
     9781 gcaacctcgc caccaactcg acgtggaacg acacctggct caacgaaggg ttcacgacgt
     9841 acgtcgagta caggatcatg gagcagctgc gcggcagcga attttccgac gtgctgtggt
     9901 tcctcggccg caaggaagtc gagcgcgccg tcgcgaagta cggcagcggg ccagcgacgc
     9961 acctcgcgca tcactacggc cgcgacaccg acgccgagga aattccgagc gggctcgcat
    10021 atgacaaagg cgcgctgttc ctacggacgc tcgagctggc gtacggccgc gccgcgttcg
    10081 acacctggct gcgcggctgg ttcgatcgtc acgcatttca ggccgtcgac tcgaagcagt
    10141 tcagcgccga gatcaagccg ctcggcagca aggtcgatat cgcgacgtgg gtcgatgcgg
    10201 gcggcttgcc ggcggatgcc gcgcccgcgc cgtctgcaca tgcagccgcg atcgaacagc
    10261 tcgcgacgag cggcggcgac gtcgacgcgt cgagctggaa cacgatggat tgggtcgtgt
    10321 acctcggcgc gttgccggaa acggtgccgg ccgcacgcct gcttgcactc gacgcgcgct
    10381 atcacctgac tggcacgagc aacgccgaga tcgcgatgca ctggttgccg ctcgtcgtcc
    10441 gcgcggatgt tcgcgatgct gctcctgcgg tcgaggcgtt cctgtcgaag gtcggccgcg
    10501 tgcgcatggt gcggccgctc tacaccgcga tgatggcggg tacggacttc tggcacgcgc
    10561 tcgcaaagtc gacgttcgaa cgcgcgaagc cgaagtacca cccgatcacc cgcggcgcga
    10621 tcaccgacat cgtaaagtga tcacggaatc tcgatgcagc cgcgttcttg cccatcaccc
    10681 atgaagtgat tcggcgcgcc gccattgcga tcgaggattg gcgtcgccgt gcccatgagt
    10741 ggtgacgtcg ccgtcggcgt gaaatcatcc ggcgtcgtgc ccgcgatgtt ggcgacgcct
    10801 gggtcggccg gcatgagcac cgacggcatg tcgatcatcg cgctatcgca cgtgccgctg
    10861 cacagcgagt tgctgctcac ggtttcgtac gcgatatcga agccggtgcc gttgttatcc
    10921 gcttgcagcg cggacgggtt gttcgcgatg tagaggttgt tgcgcagcac gatgacgcgt
    10981 ggaccactgc gccggatcgc cgccagcgac gagcgtccgt tacgcacgaa cgtgttgttg
    11041 aagatccaca accgaccgtt atccgagccg ccggcgtaga taccgctgta ttcctggtcg
    11101 acgatcaggt tgccgacgat ccagaggtcg ccgccgccct gcgatgcgat gtagatacct
    11161 tcgtcgtcgc ggtcggtgtg gtttggcgag atcatgacgt tgtcgctgat ctcggtgacc
    11221 ttggcggcga cgtagatgct gtccatcacc tgatcggtcc acgtgttgct gacgatgtgg
    11281 tgtgcggtgc cgccgtgcag ccgcacggac gcggcgtagc agttgcgctc ggcgacgtcg
    11341 ggatcctcga agcgattgcg ctcgaggatc aacgtgccgc cgtcgacgcg catcgcacca
    11401 ccctcgagct gggcgttgga gatgccgcaa ttgttcgtcg tgaggtcttc gttgcggtag
    11461 ttgaacgcga agttgcggaa cgtgatgccg gcgaccgcca ccgtgccgtt gacgatgcgc
    11521 aacccgtagc ggccttccgg gctctcccaa ctagcggcca gtgcgagggt cacgtcagcg
    11581 ctcgtcccgt cgatcacgac gtcgctcgtg atcgtcggga gctccccgag gttgtcatcg
    11641 agatccacga ccgtgcccgg cgtgaacacg gacggatcga acgtgatgat gtcgggcccg
    11701 ggtgcggtca tcgcctgtgc gagcgcttcg cgaaagctga gcttcgagcc cgtattgctc
    11761 tcgtccttct ccgtcgtgac cacgatcggc ccgttcacgc cgccgtacga cagcgtgagc
    11821 ggcgtggctg ctggtgtcgc tgacagcttg ctgccgctcg tcgtgaacac agctgccgcg
    11881 ttgacctgga ggtcgctcgc cgtgagccac ggcagcgcat cgaccacgaa gcggtacgtg
    11941 gcctcggtac ccggcgcgag cgtcgttggc tggaggtcga acacgccttc ccgacgatcc
    12001 tcgtcacgat ggcgaatctc tttgtcgtga ccgccgaacg tcagccgcgc gccgacgtcc
    12061 gtgatctcgg tcgcgagatc gttgcgcagc accgccgtga cctcgaagcc atacttgccc
    12121 gggcgcacct gggtgacggt gctcgtcacc gatgtgaagt agacgttcgg tgtcatcggc
    12181 ggggcatcaa cgccagcgtc gacttcctgc tcgcggccat gcgtgaacga acagctcgca
    12241 agcacgatca tcgcgatcga agctgccctc acgcggctat cgtaacgcga tttgcctcgg
    12301 accagcggcc ggcaaagcgt tcgtgagctg cgccactagc tcagcagggg cagccgaaga
    12361 acggtagctc caggcgagag agctcctcgc tatttgcgcg cacgaccacc gtgtcgaggc
    12421 cgagccacgg cttgccgatc agctcgagct cgccgtcgtt gtcgatgtcg acgagctgat
    12481 cgatctccca gagatcatcg agcttgcgca gctggaccgg tgcgagcgtg ccatcggtct
    12541 cgacgcggaa caggccccac acgttgacgg tcgggtcgcc gcagctgtga tcgagcgagc
    12601 catggatcga gacccaggtc acacccgtcg tcgggtgacg gactacacgg gtgtcgaagt
    12661 tgacgtactt ctcgtcccac cagttgccgt gcgcttcacc gtccgcgtcg tactccgcat
    12721 agaacttcgt gtactcggat tgcgtgtcat gcgccgcctc cgatgcgatc agcgcagcac
    12781 gggcgcggct cgcaagcgtc tcgtcctcga tggaatccgg cacgaccacc gcaggcagcg
    12841 ccgcatcgcg tgcaaacgta ccgtcacaat cagcgagctt cgccgcgagc accttcgcgc
    12901 cgctatcgaa cacggtcttc gcggtccact tcgaagcttc gatgcccgcg tagccggtgt
    12961 caccggtgag ccgtgcgacg acggcgaacc cggtcaccgt cgtgcggcag ttgttgtcga
    13021 caatgacgtc gcggtcctgc cagcgccgat agccaatcgg cacgtcgcta tctcttacgg
    13081 ttgcgatcgc ggattcgacg ccgtcggcac tgtggaggcg cagcttgcca tgcttgggca
    13141 tcgcgacatc ccggccatca ccgacctcgg tgagccgcat gtacgtcgcg ccgcccgcag
    13201 aaaacaccag cccgagctcc atggagcgca cgggttgcgc gatcgccacc ggcttctcga
    13261 cgatcggcgt gaccaccggt tcgaccttcg ggagcacggg catgcgaaac gtgacacgtc
    13321 tcgtctcgtg cgtgtcgctg ccgaggctaa ctgccgcgcc gatggcggtg aggccgagcg
    13381 cggtaagcaa catgcgacgc atgtgaggac gtgcaggcgt caggagcgcg gggcgaagcg
    13441 ggtcggtcat cgcgagcagt ttgtgcaagc ggtacgccga cgagcagatc cgaggcgacg
    13501 cgcccacttg cataaccgtg gtcgcgacga gacacggtga gtgcgcctgt gcagacacga
    13561 gggaatttcg cgcccggctc acgcgttcgg ggggaccgcc tacagtagaa tgtcgttcgt
    13621 gcgtgttctg accacgctgc tgctgctcac ggggctggcg cacgccgacg tcggcgttgt
    13681 cacgaccggc gagccgatga tgcaggcgcc cgtgctggcg caagtgcaag gctggcttca
    13741 acagcatcaa tacggcctgg tcgccgtgcc gctcggtgac gcggcgaact ccttcatgga
    13801 ctgcttcgtg atggaggaca tgggttgcgc gcagaagtcg ttcgagaagg actcgaaggc
    13861 gtcgaacctc atctacgtgc gggcggacct catggcgggg caggcgcgcg acttcaacct
    13921 gatcgcgtac tggttcgtca aaggccacga gcccgtactg cagaagcgcg agtgccacgg
    13981 ctgcgatgat gcgaagctcg gcccgatcgt cggttcgctg atgaccgatc tcgagaaggg
    14041 tatctcgtcg accaagggcc acctcaagat cacgtcggtg ctcggtggtg tgtcggtgac
    14101 gatcgacaat gtctcgctcg gcgcggcgcc gctcgagcac gacctcgatc caggcccgca
    14161 cgaaatctcg ttcctccatc gcggcaaggt gctcgagaca aagccgctga agatcgagcc
    14221 cggtgccacc gtcgagatgg ccgccccgac gctgacgatc gaagcaccgc cgcctgccaa
    14281 gcagcgttcg cgtgcgctgc ccgtctcgct cgcggtcgtc ggtgccgcac tcgccgtcac
    14341 cggt
//
LOCUS       DASUFX010000578         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1066274, whole genome shotgun sequence.
ACCESSION   DASUFX010000578 DASUFX010000000
VERSION     DASUFX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066274"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFV99_23245"
     CDS             complement(<1..657)
                     /locus_tag="VFV99_23245"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HEX5062314.1"
                     /translation="MGGNAYPSDPLHGKLGLMRFWMVVLLALAACSDDTPKTPDEILF
                     ALRQLPGVHDATEMPTQSAGYHYYVIHFEQKVDHNDPNSATFLQEVSLLHHDELHYPM
                     VVHTSGYWDYYLDRVVELTALLGANQISIEHRYFGESRPTPTDWTKLTIDQMAADQHE
                     IVTALKKIYEGKFLTTGGSKGGMTATYHRRFYPDDVDGTVPYVAPISFAAPDARYAGF
                     L"
     gene            656..1750
                     /gene="ychF"
                     /locus_tag="VFV99_23250"
     CDS             656..1750
                     /gene="ychF"
                     /locus_tag="VFV99_23250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5062315.1"
                     /translation="MALSVGIVGLPNVGKSTVFNALTAGKAEAANYPFCTIDPNVGVV
                     PVPDARLKRIESHIKTQKVIPAIVEIVDIAGLVKGASQGEGLGNKFLANIRETNAVLM
                     MVRCFDDPNVIHVSGSVDPMRDIDIIDLELALADVETAEKRVKKAQGAAKTGSKEGKI
                     ELALAEKVFNHLQAGKAARSLQLSDDEKKQVATFGLLTAKPVLYCCNVGEADLPAGNQ
                     WSDAVKERAKKEGAGVVILCGKVEAELAELGEADRVELLKSYGLEEPALSSLARECYR
                     LLGLQSYFTAGEKEVRAWTIRKGALAPEAAGVIHTDFEKGFIRAQVYGLADLDQFGSE
                     AGIKAAGKLRMEGKEYVVQDGDIMHFLFNV"
     gene            complement(1754..2563)
                     /locus_tag="VFV99_23255"
     CDS             complement(1754..2563)
                     /locus_tag="VFV99_23255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5062316.1"
                     /translation="MQREQFLRAFHDAQPGITSRAMARAGSYERLAAKVPKGRVLDLG
                     CGDGTLLRLLGPHAIGIDVSHGELVAAKAVVTAGGTGAGAVVVQARAQELPFRDRTFD
                     AATCHLAFMLFDEIECVVAELARVLKPRAPFVALLGGGPTAGGGASAGGGASAGGGAG
                     AGGDDAFHRFTHILSRTKAASPYTDIFRGAGDKRASSEAGWRELFRGWSEPQFERWPL
                     DLGGTFDEVWTFLAASYQMRDHDYVRDRLREEFPGDYVPCTVVGFCATVTR"
     gene            2610..3482
                     /locus_tag="VFV99_23260"
     CDS             2610..3482
                     /locus_tag="VFV99_23260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5062317.1"
                     /translation="MAVAVSAERKSSLLAGVAMVAIAAASWGTWSLFLRPTGLSPLVT
                     SPLMFAMMAVVAFPFTLRAAPARWDRRTVLLLLANTCTDALNVITFFGAMAYTTVAIA
                     VLTHYLAPILIALASPRIEGTTTRGARPAAVVALAGLVIVLEPWRGAADGVVIGSLLG
                     VASAVAYAANVFVVRRLAERIGPARVMSYHSALAAIVMLPVAVSGLATVSWADVGLLA
                     AGSTTIGALSGIIFIAGLTRIGAARTAVLTFAEPLVAVAVGALVWSEPLRPLAAIGGA
                     LVLVAGIHVARQAR"
     gene            3485..4519
                     /locus_tag="VFV99_23265"
     CDS             3485..4519
                     /locus_tag="VFV99_23265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5062318.1"
                     /translation="MRMLPRVTIAMPAYNEAQYIEACITSVQRQDYPAELVEILVADG
                     RSTDRTREILAKLAAADPRIVMIDNPERLQAAGLGQIVKQAMGDVVVRMDVHCEYAPD
                     YVRKCIETLERTGADNVGGAQRAKAKTAFQRALCAALTSPLGVGGAKYRSADAEGFVD
                     TVFLGAFRRKVFETVGLWDPAAITNEDSELNQRILENGGQVYLSRDIVVHYFPRDSFR
                     SLAKQYFKYGRGRAHTLLKLRRFPTVRPLLPFLMTTGAAALVALPPLWPLAPAAFATY
                     ALAAGAEAVRVGRRLGGAAIPLVWAMFPVLHLSHGAGFAAGLARYLRKPDWPAEPERI
                     GPRRAHLRAV"
     gene            complement(4516..>5176)
                     /locus_tag="VFV99_23270"
     CDS             complement(4516..>5176)
                     /locus_tag="VFV99_23270"
                     /inference="COORDINATES: protein motif:HMM:NF023212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HEX5062319.1"
                     /translation="AMAPPDLADGSGPTINSIAGEEGSAPLRLPRGAIAIGQYQAAAV
                     PAGRRAPDDMFAPRYLVELGKCVEQVLAAEAPIHIDLLARRVAAYFGIGRVSQRHLEQ
                     VQSALLGRGKWGTEKNVVWRLDQDTASVPAVRVAGTTGTSKRDIGEIPLCELAAAARI
                     VVERVNGVTTNDLVRDCARLLGFARMTDKVSERVTLGIRLAAARELIAIDGGKAHLLL
                     A"
BASE COUNT          849 a         1729 c         1740 g          858 t
ORIGIN      
        1 gaggaagccg gcgtaccgcg cgtcgggcgc ggcgaacgag atcggggcca cgtacggcac
       61 ggtgccgtcg acgtcatccg ggtagaaccg gcggtggtac gtggcggtca tgccgccctt
      121 gctgccgccc gtcgtgagga acttcccctc gtagatcttc ttcagcgcgg tgacgatctc
      181 gtgctgatcg gctgccatct ggtcgatcgt cagcttcgtc cagtcggtcg gggtcggccg
      241 cgactcgccg aagtagcggt gctcgatcga gatctgattc gcgccgagaa gcgcggtcag
      301 ctcgacgacg cgatcgaggt agtagtccca gtaccccgag gtgtggacga ccatcgggta
      361 gtgcagctcg tcatggtgga gcagcgaaac ttcctgcaga aacgtcgcgc tgttcgggtc
      421 gttgtgatcg accttctgct cgaaatgaat gacgtaatag tgatagccgg cgctctgggt
      481 cggcatctcg gtcgcgtcgt ggacgccggg caactggcgg agcgcgaaca ggatctcgtc
      541 cggggtcttt ggcgtgtcgt cgctgcaggc cgccagcgcg agcaaaacca ccatccagaa
      601 gcgcatcagg ccgagcttgc catgtagtgg atcgctgggg taggcattgc ctcccatggc
      661 gctgtcggtc ggcattgtag gcctccccaa tgtgggcaag agcactgtgt ttaacgcgct
      721 cactgccggc aaagcagaag cggcaaacta tccgttctgc acgatcgacc cgaacgtcgg
      781 tgtggtgccg gttcccgatg cccgcctgaa gcgcatcgag tcccacatca agacgcagaa
      841 ggtgatcccg gcgatcgtcg agatcgtcga catcgcgggt ctcgtcaaag gcgcgtcgca
      901 aggtgaaggc ctcggcaaca agttcctcgc aaacattcgc gagacgaacg cggtgctgat
      961 gatggtgcgc tgcttcgacg atccgaacgt catccacgtc tcgggcagcg tcgatccgat
     1021 gcgcgacatc gacatcatcg atctcgagct cgcgctcgcc gacgtcgaga ccgccgagaa
     1081 gcgcgtcaag aaggcgcaag gcgcggcgaa gaccggcagc aaagaaggca agatcgagct
     1141 cgcgctcgcc gagaaggtgt tcaatcacct gcaggccgga aaggcggcgc gctcgctgca
     1201 gctcagcgat gacgagaaga agcaggtcgc gacgttcggc ctgctcaccg cgaagccggt
     1261 gctgtactgc tgcaacgtcg gcgaggctga cctgcctgcc ggcaaccagt ggagcgacgc
     1321 ggtcaaggag cgcgcgaaga aggaaggcgc tggcgtcgtc atcctgtgcg gcaaggtcga
     1381 ggcggagctt gccgagctcg gcgaggccga ccgcgttgag ctgctcaagt cctacggcct
     1441 cgaggagccg gcgctgtcgt cgctcgcgcg cgagtgctat cgcctgctcg gcctgcagtc
     1501 gtacttcacg gcgggcgaga aggaggttcg cgcgtggacg atccgcaagg gcgcgctcgc
     1561 gccggaagcg gccggcgtga tccacacgga cttcgagaag ggcttcattc gcgcccaggt
     1621 ctacgggctc gcggatctcg atcagttcgg cagcgaagcg ggcatcaagg cggccggcaa
     1681 gctgcgcatg gaaggcaagg agtacgtcgt ccaggacggc gacatcatgc acttcctgtt
     1741 caacgtctga tgctcatcgc gtgacggtcg cgcaaaaccc gacgaccgtg cacggcacgt
     1801 aatcgccggg aaattcttcg cgcagccgat cgcgaacgta atcgtggtcg cgcatctgat
     1861 agctggcggc gagaaacgtc cacacttcgt cgaatgtgcc gccgaggtcg agcggccagc
     1921 gctcgaactg tggctcgctc cagccgcgaa acagctcgcg ccatccggcc tcgctgctcg
     1981 cgcgcttatc gcccgcgccg cggaagatat cggtgtaggg ggacgcggcc ttggtgcgcg
     2041 acaggatgtg cgtgaagcgg tgaaacgcgt cgtcgccgcc ggcacccgca ccgccgccgg
     2101 cactcgcacc gccgccggca ctcgcaccgc cgccggccgt cggaccaccg ccgagcagcg
     2161 ccacgaacgg ggcacgcggc ttcaacacgc gtgcgagctc cgcgacgacg cactcgatct
     2221 cgtcgaagag catgaacgcc aggtggcagg tggcggcgtc gaacgtacga tcgcggaacg
     2281 gcagctcttg cgcccgcgcc tgcacgacca ctgctcctgc tcctgttcct cccgccgtga
     2341 ccactgcctt cgctgccaca agctcgccgt gcgagacatc aatcccgatc gcgtgcggac
     2401 cgagcaacct gagcagtgtt ccatctccac aaccgagatc cagcaccctg cccttcggaa
     2461 ccttcgctgc gagtcgttcg taactgcccg cgcgtgccat cgcgcgcgac gtgatgcccg
     2521 gctgcgcatc atgaaatgcc cgcaggaact gctcgcgctg catcacaccc tgcatacaat
     2581 cacgctgtgc tcgcgaagtg gtaccgctga tggcagttgc cgtgagcgcg gagcgcaagt
     2641 catcactgct cgctggcgtc gcgatggtcg caatcgccgc cgcgtcgtgg ggcacgtgga
     2701 gcttgttcct ccgcccgacc ggactgtcgc cgctcgtcac gagcccgctg atgttcgcga
     2761 tgatggcggt cgtcgcgttt ccgttcacgc ttcgcgccgc gcccgcacgc tgggatcgcc
     2821 gcaccgtcct gttgctcctc gcgaacacgt gcaccgacgc gctcaacgtg atcacgttct
     2881 tcggcgcgat ggcgtacacc accgtcgcga tcgcggtgct cacgcactac ctcgcgccga
     2941 tcttgatcgc gctcgcgtca ccgcgcatcg aaggtacgac cacgcgtggc gcgcggccgg
     3001 cggcagtcgt cgcgctcgcc gggctggtga tcgtgctcga gccgtggcgc ggcgcggcgg
     3061 atggcgtcgt gatcgggtca ctgctcggtg tcgcgtcggc ggtcgcatac gccgcgaacg
     3121 tcttcgtcgt ccgccggctc gcggaacgga tcggacccgc gcgcgtgatg tcgtaccact
     3181 cagccctcgc agcaatagtg atgctgccgg tcgcggtgag cggtcttgcc acggtgagct
     3241 gggctgacgt tggcctgctc gccgcgggtt cgacgacgat cggcgccctg tcgggcatca
     3301 tcttcatcgc aggactcact cgaatcggtg ccgcgcgtac cgcggtgctg acatttgcgg
     3361 agccacttgt tgccgtcgcg gttggtgcac tcgtatggag cgagcccttg cgcccactcg
     3421 ccgcgatcgg cggcgcgctc gtgctcgtgg ctggtatcca cgttgcacga caagcgcgct
     3481 agccatgaga atgctccctc gcgtcacgat cgcgatgcct gcgtacaacg aagcccagta
     3541 catcgaggcg tgcatcacga gcgtgcagcg gcaggactac cccgccgagc tcgtcgagat
     3601 cctcgttgcg gacggtcggt cgacggaccg cacgcgcgag atcctcgcga agctcgccgc
     3661 cgccgatcca cgcatcgtga tgatcgataa tcccgagcgc ctgcaagccg cggggctcgg
     3721 ccagatcgtc aagcaagcga tgggcgatgt cgtcgtgcgc atggatgtcc actgcgagta
     3781 cgcgccggac tacgtgcgca agtgcatcga gacgctcgag cggaccggcg ccgacaacgt
     3841 cggcggtgcg caacgcgcaa aggcaaagac cgcgttccag cgcgcgctgt gtgccgcgct
     3901 gacgagcccg ctcggcgtcg gtggcgcgaa gtatcgctcg gccgatgccg agggcttcgt
     3961 cgacaccgtg ttcctcggcg cgttccgccg caaggtgttc gagactgtgg ggctgtggga
     4021 tccggccgcg atcacgaacg aggacagcga gctcaaccag cgcatcctcg agaatggtgg
     4081 ccaggtctac ctgtcgcgcg acatcgtcgt gcactacttc ccgcgcgaca gcttcaggtc
     4141 gctcgcgaag cagtatttca agtacggtcg cggccgcgca cacacgctgc tcaagctccg
     4201 tcgctttccg acggtgcgac cgttgttgcc gttcctcatg acgaccggag ccgctgcgct
     4261 cgtcgcgctg ccaccgctct ggccactcgc gccggcagca ttcgcgacgt atgcactcgc
     4321 ggcgggcgcc gaggcggtac gcgtcggccg ccggctcggc ggagcagcaa tccctctggt
     4381 gtgggcgatg tttccggtcc tgcacctctc gcacggcgcc ggcttcgcag ccgggctcgc
     4441 gcgctatctg cgcaagccgg actggcccgc ggagcccgag cgcatcgggc cgcggcgagc
     4501 acacctgcgc gccgtctagg cgagcagcag atgcgctttg ccaccgtcga tcgcgatcag
     4561 ctcgcgagca gcggcgaggc gaatgccgag cgtgacgcgc tcggagacct tgtcggtcat
     4621 ccgcgcgaag ccaagcaggc gagcgcagtc gcgaacgagg tcgttcgtcg tcacgccgtt
     4681 gacgcgctcg acgacgatgc gtgccgcggc agcgagctcg cagagtggaa tctcgccgat
     4741 gtcgcgcttg gacgtgccgg tcgtgccggc aacgcgcacc gctgggacac tcgcggtgtc
     4801 ctggtcgagg cgccacacga cgttcttctc ggtgccccac ttgccgcggc cgagcagcgc
     4861 gctctggacc tgctcgagat ggcgctgact gacgcggccg atgccgaagt aggcagcgac
     4921 gcgacgcgcg agcagatcga tgtggatcgg cgcttcggca gcgagcacct gctcgacaca
     4981 cttgccgagc tcgacgaggt accgcggcgc gaacatgtca tccggcgcgc gacggccggc
     5041 gggcaccgcg gcggcttggt actgaccgat cgcgatggca ccacgcggca agcgcagcgg
     5101 cgcactgccc tcctcgcccg cgatcgagtt gatcgtcggg ccggagccgt cggcgagatc
     5161 cggcggcgcc attgcg
//
LOCUS       DASUFX010000579        14987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1482977, whole genome shotgun sequence.
ACCESSION   DASUFX010000579 DASUFX010000000
VERSION     DASUFX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482977"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VFV99_23275"
     CDS             <1..1263
                     /locus_tag="VFV99_23275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5062320.1"
                     /translation="PMNRLLQGDVGAGKTAVAFAAALHVARAGRQVAVMAPTELLAEQ
                     HAETWKKWAALINGTAKQKLRVEILTASTPKGVRQSLLALLAAGEVDILIGTHSLIAE
                     GVGFHALGLVVIDEQHRFGVAQRAKLRDKGDGQGAPHLLVMTATPIPRTLALTAYGDL
                     DVSILDELPPGRLPVQTKIASGKRGEAAAYKLITERVANGERAYVVCPKVEDDGESEV
                     DWKDATTVADEMAAALPQLRVGLVHGRLDGDTRDRVMRAFKAGELDVLVATTVIEVGV
                     DVPAATVMVIHDAERFGLAQLHQLRGRVGRGSGDAHCVLVTHGTISEDGRRRLDAMVA
                     TTDGFKIAEEDLALRGPGELLGPRQAGVPRLRFGSLTQHTELLLEARRHAERILDEDP
                     GLVRPEHEALRHALTRRLAQSVYGAESG"
     gene            1365..1784
                     /locus_tag="VFV99_23280"
     CDS             1365..1784
                     /locus_tag="VFV99_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062321.1"
                     /translation="MALAFACADSSMGDDDGNGSGSDMGSSQNQCGDGTCAPSEIGFC
                     MEDCGNGSNGGNNSSCGNGQCETTKGENGASCPQDCSSGSGSGSGSGSGSGSGSGSST
                     LDCADQNAIFGCLLCTGLQMCTPPYTVADCTACGFGP"
     gene            complement(1801..2115)
                     /gene="cutA"
                     /locus_tag="VFV99_23285"
     CDS             complement(1801..2115)
                     /gene="cutA"
                     /locus_tag="VFV99_23285"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5062322.1"
                     /translation="MSVIVVFSTFPDPAKAAEVARTLVTEQLAACVNLLPAVRSIYRW
                     QGEISDDTETLAVIKTTRERFEAMRTRLVELHPYEVAEVIALPVEAGHAPYLAWIVSA
                     VA"
     gene            2146..3390
                     /locus_tag="VFV99_23290"
     CDS             2146..3390
                     /locus_tag="VFV99_23290"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5062323.1"
                     /translation="MLIVQKYGGTSVGSIERMKNVAARCLRSQREGHQVAVVVSAMSG
                     ETNRLLKLVDEIHDDHNDREVDVIVSTGEQVSVGLVALAIRTLGGKARSFLGHQCKIL
                     TDSSFARARIVDIDAKPIREAMEAGEIAVIAGFQGVDEKGNITTLGRGGSDTTGVAIA
                     AALKADVCEIYTDVDGVYTTDPNIVPTARKIERISYEEMLELASLGAKVLQIRSVELG
                     MKYGVPIHVRSSFSDVPGTWVVPEEAAMEKVIVSGVTASRDESKITCEDLPDQPGIAA
                     RMFAPLADANISVDMIVQNQAYDGTTDLTFTLPKIDRKRAIELLKEKVPELVGKEGER
                     IAYDDEISKVSVVGVGMRSHAGIAKNMFTLLANEGINIQLISTSEIKISCVIARKYAE
                     LAVRTLHDGFGLSLPPSERAGL"
     gene            3417..4106
                     /locus_tag="VFV99_23295"
     CDS             3417..4106
                     /locus_tag="VFV99_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062324.1"
                     /translation="MGGCTNCKGKAGCDHRKGAMIESVDRALETLYPTRTWGEPDDTG
                     AAERGMLRDELDALADELAQELGAATFVREGGDDEPCDYIYVLCMGRTPCVIQVRDHA
                     VPAPAEWNDASAIEELYLRVVISQRARVAAVQQVGVDLAKGEGGWLVRERPRAGVYDA
                     PLLHRMQKLVAILPAYELLHVDFGEIAHAPPGYAAGTWRELFGGEPSIANYLFYPQPT
                     TMVATSFLERT"
     gene            4103..4816
                     /locus_tag="VFV99_23300"
     CDS             4103..4816
                     /locus_tag="VFV99_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062325.1"
                     /translation="MIEEEVRDALKALVDEIGATSARIVVDDDIRTGVPARTLALGGG
                     EYLRVELPTRIERAGSESGREHDIEAAFERVTRQLRGIRRRWEVARLPEVTVAPGATP
                     TGGLVKERIESYMQALAGIDRAQNAFVTKGTQLIASAREADEQEASRWPFLARRALAT
                     HAPGSSHGEVVDPDAYAMSFWYDASLVVLLTEPYAIDFVRHRCRQVARELCNLLPLLD
                     PDPDAPAAIGPRPRRPTQQ"
     gene            5093..5854
                     /locus_tag="VFV99_23305"
     CDS             5093..5854
                     /locus_tag="VFV99_23305"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX5062326.1"
                     /translation="MLLSPRQTVELFHLVFVRALFSNAADKTLLAIKGGINLRFFFQS
                     VRFSEDLDLDVAKMAKGTLENRVDKLLVSPAVTAPLRSRGITLKEVSKPKQTETVQRW
                     KIALATAGGAFSEHTKLEFSRRGGTDDADLDPVDPAIAKAYALPSFLATHYRCAEAVR
                     QKIGALAGRTQTQARDIFDLHVLFARPDAPEDLEEQEPGWFAKAVENAGHVSYEQYKS
                     TVIAFLDPDHAALYSAEETWDAMQLGVIESIEKLR"
     gene            5881..6462
                     /locus_tag="VFV99_23310"
     CDS             5881..6462
                     /locus_tag="VFV99_23310"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX5062327.1"
                     /translation="MALGLPTLETADVASKFRISTKAANKLLGRLSRAGLIMRLRAGL
                     WLTSRTPPSAYSLVEPLAAPFGAYVSLHSALYHHGMIEQIPAVVYAVSLGRTRRIRTP
                     VATFSFHHIAPELFDGFKTMSGDVKLATPEKALFDVAYFSGGRSRLFAHLPELELPKR
                     FSRSALDRWVARIPAARRRSMTASRIQSLLTPR"
     gene            complement(6459..6974)
                     /locus_tag="VFV99_23315"
     CDS             complement(6459..6974)
                     /locus_tag="VFV99_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062328.1"
                     /translation="MIKSALLGVAIALVLFGAVTVVLRWRYQHQQEERAAERLPELRA
                     EAFKAAKLCDSVVDESLAPAMAMAMSKFGFYLATDRKRLSETLTTVVEPVISSRQMAC
                     SNAKTSLELFIEKSPTLDAFAMPRLVRAHAHLVRLDALEHELDATRDAIDHGANDAEL
                     LAHFARLRSRP"
     gene            complement(6971..8524)
                     /locus_tag="VFV99_23320"
     CDS             complement(6971..8524)
                     /locus_tag="VFV99_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5062329.1"
                     /translation="MQLLASYLHGTWSSGSGSRSPLVNPATEEVLAEVATGGHDLAAA
                     FAHARSEGAPALGLLTFAQRGALLGSLAKAIHGAREELIALAVANGGNTRSDAKFDID
                     GGAVTLSHYAELGAKLGNATMLGDGEPIQVGRTARMGGQHVWSRSRGLAVHINAFNFP
                     AWGTCEKLACAVLAGVPVISKPATATALVAHRLAQLFEPLLPKGVLQMLIGPAGTLLD
                     HLSAGDVVAFTGGSATARMLRTHPKIVEHGVRLNVEADSLNAAILGPDVDLSSEAGNL
                     FLADVVRDMTQKTGQKCTAIRRVLVPEVKLKDVCDAIRERVAAIAIGDPSREDVRMGP
                     LATAQQRDDVKTGIARLAAATESVHGGNGEVTPVGVPAGKGYFVGPVVRSTTSPMECA
                     PLNEYEVFGPVSTVGSYNGTAVFAAEFVARGEGCLVSSAYSDDRDWVATFVSAASSWA
                     GRLYLGSSKMASQSPGPGTVLPSLLHGGPGRAGGGEELGGERGMHFYMQRCALEGDAS
                     VLKSLVSGS"
     gene            8566..9603
                     /locus_tag="VFV99_23325"
     CDS             8566..9603
                     /locus_tag="VFV99_23325"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX5062330.1"
                     /translation="MSSSLQKRLLAGLLFLAVTVVGGAAGFYAIGARAGADWTFWDCL
                     YMTVITVTTVGFGETLNHMDAVEYARGFTMLLLLFGTGSIVFFASTITAFIIEGDLKN
                     VLFANKLKKRMKRMKDHVVVCGAGSTGRNVIEELIKTGVPVIAIDVNEVELKEIADRN
                     PKAEYAYIVGDATDDDVIAQVNLKGARGLVAALSSDKDNLYLTVSARQDNPTARIVAR
                     CAELSHVDKIKKSGADAVVSPNYIGGLRLVSEMLRPSVVRFLDEMLRDRVAYRIEEIT
                     LGDSAAELGSTLRDARVRERYGMTVLAVRRREGEGWTYNPDAAEKIGPGMTLVVLGSP
                     EQVSQLKDATK"
     gene            complement(9584..10384)
                     /locus_tag="VFV99_23330"
     CDS             complement(9584..10384)
                     /locus_tag="VFV99_23330"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5062331.1"
                     /translation="MNRERLQRIRNFQSEDWWPALVIRPISIAIMLVIADWRFVTANR
                     LTTLSNLCKLAGAWLLLRPEHWVLCVVLLQLGLIFDHLDGTLARYRRTFTKLGSYYDK
                     VSDMITWAVIVMVLGWQAYRTSADAMYLVFAGAAVAALELRGYMKWLCQAETERLRWL
                     EAKQDPAGTVARYTAPIVIKPPPERSARDWAIWFAKKVAVVFVFEEADLWFWLCLGLL
                     IDRLDLVLWLLMLTQCAGAAAMVIVRAVRMARADRQIHALESTTSSRL"
     gene            complement(10417..11403)
                     /locus_tag="VFV99_23335"
     CDS             complement(10417..11403)
                     /locus_tag="VFV99_23335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5062332.1"
                     /translation="MRKRLWRFAPRALAYGPLLAQLVITRRCNLTCGYCNEYDTFSPP
                     VPTDVLKQRVDKLASLGTLFCEYTGGETLLHPDLIELVAYAASYNFNERWIITNGYLL
                     SAEIVKQLNDAGLTHLQISIDGVTPNDTTVKVLKALRKKIEHLQNHAKFTVQINAVLG
                     AGNDEEVLEVMKFARDAGFRPRASIIHDGDGGMILDERGKQLMQEVSEIVGKRFNESQ
                     DYRARLIETGRADFKCRAGARYLYVDEHGVVHWCSQQRGVFTKPLLDYTFEDLREQFY
                     TKKSCAPTCTVGCVRTASRWDQWRGQDREFVAPPDVALSALTKRSTERENSG"
     gene            11509..12393
                     /locus_tag="VFV99_23340"
     CDS             11509..12393
                     /locus_tag="VFV99_23340"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5062333.1"
                     /translation="MSGTFHRVFRAITRSYPYTTPAYEINVALAPLLDRETPLWLEAN
                     GYRGSPRMMLNVRNRFQRKIFYFPVAYARHWLQAPLGEYMRQRLRPGDWFVDIGANIG
                     YYSLLAAGLVGAEGTVMSFEPEPGAFESLTRTMALNECGQVQPHNVGLSNREGKLQLY
                     RARDTSHSLVEATEKLGNFRGTVDVDVYPLDRFPPAAALDGRVRLIKVDVEGEEARVA
                     QGMLETLARIGKPPVWSEVRGPEGSERAPDTFAPMLEALSQLGYRAFRWRKDGITEVT
                     QHDVRKREDILFAVDPNA"
     gene            12390..13121
                     /locus_tag="VFV99_23345"
     CDS             12390..13121
                     /locus_tag="VFV99_23345"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5062334.1"
                     /translation="MIEQAVILAAGKGTRLRETADALPKPLTTVGGVPLIKRTILTLA
                     DAGVRRVVVVTGFMADQVRATVSGDPTYAARGLEIEFVHNAEFELSNGISVMLGGKAA
                     GGAFVLSMADHIYTVAIARLLVGCNLDTADLVLATDPRVDQVLDIDDATKVRTQDGRI
                     VEIGKQIATYDRIDCGVFAVGPRLIDALAEERAARGDCSLSDGVRRLAQAGRARVIDI
                     GNEPWQDVDTPADREHAERALGKSR"
     gene            13196..14662
                     /locus_tag="VFV99_23350"
     CDS             13196..14662
                     /locus_tag="VFV99_23350"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX5062335.1"
                     /translation="MSTRVNRGLAWLGFASTAVGLLDIVAMLLILNFWISKADLGIAT
                     MAVWIFPILDQATELGLSAALIQRDDHTETRTSTVFWVNLMLSLAAFLLLVAGAPPLA
                     KHFYHQAIVGWMLIAYGSKLLLQNAYIIPTAMMKRELRFKELSIIKLLANIAEFFGKV
                     GFAWAGFGIWCYVLGPLARTLVYAVGLQWCSPYRPRMSFNLADARDYVRFGLRTSGSQ
                     ILFYFYTNIDYPIVGVFFGKEALGLYRYAYEIVLEPVRILSNVVVDIAFPAFARLRHS
                     KEQLVAQFVSFTKLNLITVMSYAAVVFVAAPELIAVLFPDYAGAETAARILCGVAVLR
                     AVGFVMPPLLDGVGRPERTFTYMTCAAVTMPILFVIGAAIGVRTHGGFESVAVAWAVG
                     YPIAFSVLAYMATQTIGWSALKYLRAVIGVVACLVVAGAMAAALRYALAGYSSGIKLL
                     ATAGTVILASGFLLAYTQGLTLRSAMRSMREPPTDPSL"
     gene            14693..>14987
                     /locus_tag="VFV99_23355"
     CDS             14693..>14987
                     /locus_tag="VFV99_23355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5062336.1"
                     /translation="MARIGLWRYLYAPFHVQMVVIRRCNLSCGYCNEFDDHSPPVPFE
                     ELCRRIDKIYELGAWAIELTGGEPMEHPQLVDLVRYAKQKGFYKVQLISNAYLW"
BASE COUNT         2632 a         4801 c         4864 g         2690 t
ORIGIN      
        1 ccgatgaacc ggctactgca gggcgacgtc ggtgccggca agacggccgt cgcgttcgcg
       61 gctgcgctgc acgtcgcacg tgctggacgc caggtcgcgg tgatggcgcc gaccgagctg
      121 ctcgccgagc agcacgccga gacgtggaag aagtgggctg cactgatcaa cggtacggcg
      181 aagcagaagc ttcgcgtcga gattctcacc gcgtcgacgc cgaagggcgt gcgtcagtcg
      241 ctgctcgcgc tgctggccgc tggcgaggtc gatatcctga tcggcacgca ctcgctgatt
      301 gccgaaggcg ttggctttca cgcgctcggc ctcgtcgtga tcgacgagca gcatcggttc
      361 ggcgtcgcgc agcgcgcgaa gctgcgtgac aagggcgatg gtcagggcgc gccgcatctg
      421 ctcgtgatga ccgcgacgcc gattccgcgc acgctcgcgc tcaccgcgta cggcgatctc
      481 gacgtgtcga tcctcgacga gctgccgccg ggccggctgc cggtgcagac gaagatcgcg
      541 agcggcaagc gcggcgaagc tgctgcgtac aagctgatca ccgagcgcgt cgcgaacggc
      601 gaacgcgcgt acgtcgtgtg tccgaaggtc gaagacgatg gcgagagcga agtcgattgg
      661 aaggatgcga cgacggtcgc cgatgaaatg gccgctgcgt tgcctcagct gcgcgtcggc
      721 ctcgtgcacg gccggctcga cggtgacacc cgcgatcgcg tgatgcgcgc gttcaaggcg
      781 ggcgagctcg acgtgcttgt cgcgaccacg gtgatcgaag tcggtgtcga cgtgccggcc
      841 gcaacggtca tggtgattca cgacgccgag cgcttcggac tcgcgcagct gcatcagctc
      901 cgcggccgcg tcgggcgcgg cagtggcgac gcgcactgcg tgctcgtcac gcacggcacg
      961 atcagtgaag acggccgtcg ccgcctcgac gcgatggtcg cgacgaccga cggcttcaag
     1021 atcgccgagg aggatctcgc cctgcgtggg cccggcgagc tactgggtcc acggcaggcc
     1081 ggcgtgcctc ggcttcgctt cggcagcctc acccagcaca ccgagcttct gctggaagct
     1141 cgacgacatg cagagcgaat tctcgacgag gatccgggct tggtccgacc ggaacacgag
     1201 gccttacggc acgcgttaac ccgtcgactt gcacaatctg tgtacggcgc tgaaagcgga
     1261 tgatcgacga gccgcgctca ctttgttgtt gatcgagtac gtccgctctg tagaagatgc
     1321 cgcccatgat gaggatcaac gcggggatct tttgcgccct ggccatggct ctcgcattcg
     1381 cgtgcgccga ttcgtcgatg ggtgacgacg atgggaatgg cagcggtagc gacatgggca
     1441 gcagccagaa tcagtgcggc gacggcacgt gcgcgccgag cgagattggc ttctgcatgg
     1501 aggactgcgg caacggcagc aacggcggca acaactcgtc gtgcggcaac gggcagtgcg
     1561 agacgacaaa gggcgagaac ggtgcttcgt gtccgcaaga ttgctcgagc ggcagtggca
     1621 gcggcagcgg aagtggatcc ggcagcggct cgggctcggg gtcgagcacg ctcgactgcg
     1681 cggatcagaa cgcgatcttc gggtgcctcc tctgcactgg ccttcagatg tgcacgccac
     1741 cgtatacggt ggcggattgc acggcgtgcg gatttggtcc ctgagcgttt ctcggtttgg
     1801 ctacgcgact gctgaaacga tccacgcgag gtagggcgcg tggccggcct cgacgggcag
     1861 cgcgatgacc tcggcgactt cgtacggatg tagctcgacg agccgcgtcc gcatcgcctc
     1921 gaaccgctcg cgcgtggtct tgatgaccgc gagcgtctcg gtgtcgtcgc tgatttcgcc
     1981 ctgccagcga tagatcgagc gcacggccgg aaggaggttt acgcaggccg cgagctgttc
     2041 ggtgacgagc gttcgcgcta cttctgcggc cttggccggg tcgggaaacg tcgaaaacac
     2101 gacgatgacg gacatgcgcc tctctatcat gtagagtgcc gcaccgtgtt gatcgtccag
     2161 aagtacggtg gcacgtcggt aggctccatc gagcggatga agaacgttgc ggcgcgctgc
     2221 ctgcgttcgc agcgcgaagg tcatcaggtc gccgtcgtcg tttcggcgat gtcgggtgag
     2281 acaaaccggc tgctcaagct cgtggatgaa atccacgacg accacaacga ccgcgaggtc
     2341 gatgtcatcg tgtcgaccgg cgaacaggtc tcggtcggcc tcgtcgcgct cgcgattcgc
     2401 acgctcggcg gcaaagcgcg ctcgttcctc ggtcatcagt gcaagatcct gacggacagc
     2461 tcgttcgccc gcgcgcgcat cgtcgacatc gacgccaaac cgattcgcga ggcgatggag
     2521 gccggcgaga tcgcggtcat cgccggattc cagggcgtcg acgagaaggg caacatcacg
     2581 acgctcggcc gcggtggttc cgacacgacg ggcgtcgcga tcgcggcggc gctcaaagcg
     2641 gatgtctgcg agatctacac cgatgtcgac ggcgtctaca cgaccgatcc caacatcgta
     2701 cccaccgctc gcaagatcga acgtatcagc tacgaggaga tgctcgagct cgcgtcgctc
     2761 ggcgcgaagg tcctgcagat tcgttcggtc gagctcggca tgaagtacgg cgtaccgatc
     2821 cacgtgcgct cgagtttttc ggatgtaccg ggaacgtggg tcgttcccga ggaggctgca
     2881 atggagaaag tcatcgtctc cggtgtcacc gcgtcgcgtg acgaatcgaa gatcacctgc
     2941 gaagacttgc cggatcagcc cggcatcgcc gcccgcatgt tcgcgccgct cgccgacgcg
     3001 aacatctcgg tcgacatgat cgtgcagaac caagcgtacg acggcacgac cgacctcacg
     3061 ttcacgctgc cgaagatcga tcgcaagcgc gcgatcgagc tgctcaagga gaaggtgccc
     3121 gagctcgtcg gtaaagaagg cgagcggatc gcgtacgacg acgagatcag caaggtctcc
     3181 gtcgtcggcg tcggcatgcg ctcgcacgcc ggtatcgcga agaacatgtt cacgctgctg
     3241 gcgaacgagg gcatcaacat ccagctgatc tcgacgagcg agatcaagat ctcttgtgtg
     3301 atcgcacgca agtatgcaga gttggcggtt cgcacgctgc acgacggctt cggactttct
     3361 ctgcctccgt cggaacgtgc agggctctag ttagctttcc aggtacagtg tcccagatgg
     3421 gcggctgcac gaactgcaag ggcaaggcgg ggtgcgacca ccgcaagggc gcgatgatcg
     3481 aatccgtcga tcgcgcgctc gagaccttgt atccgacgcg gacgtggggg gagcccgatg
     3541 acaccggcgc cgcagagcgc ggcatgctgc gcgacgagct ggatgcgctg gccgacgagc
     3601 tggcgcagga gctcggggcc gcgacgttcg tccgcgaggg cggcgacgac gagccgtgcg
     3661 actacatcta tgtgctttgc atgggtcgca cgccctgcgt aatccaggtc cgcgatcacg
     3721 ccgtgcccgc accggccgag tggaacgacg cgagcgcgat cgaagagctg tacctgcgcg
     3781 tcgtgatcag ccagcgcgcg cgcgtcgctg ccgtgcagca ggtcggcgtc gatctcgcaa
     3841 aaggggaggg cggttggctc gtgcgcgagc ggccgcgcgc cggcgtctac gacgcaccgc
     3901 tgctccatcg tatgcagaag ctcgtcgcga tcctgcccgc gtacgagctg cttcacgtcg
     3961 acttcggtga gatcgcgcat gcaccgccgg gctacgccgc cggtacctgg cgcgagctgt
     4021 ttggtggcga gccgtcgatc gcgaactatc tgttctaccc gcagccaacc acgatggtcg
     4081 cgacgtcgtt cttggagcgc acttgatcga agaagaggtg cgcgatgcac tcaaggcgct
     4141 cgtcgatgaa atcggcgcga cgtcggcacg gatcgtcgtc gacgacgaca tccgcaccgg
     4201 cgttcccgcg cgcacgctcg cgctcggcgg cggcgaatac ctgcgcgtcg agctccccac
     4261 gcgcatcgag cgcgcggggt cagagtccgg ccgcgagcac gacatcgagg ccgcgttcga
     4321 acgcgtcact cgtcagctcc gcggcatccg ccggcgctgg gaagtcgccc gtctgcccga
     4381 ggtcacggtc gcaccgggcg cgacgccgac cggcggcctc gtcaaagagc gaatcgagtc
     4441 gtacatgcag gcgctcgcag gcatcgaccg cgcacagaac gcgttcgtca cgaagggcac
     4501 gcagctgatc gcatcggccc gcgaggccga cgagcaagaa gcgtcgcgct ggccgttcct
     4561 cgcgcgccgt gcgctcgcga cgcatgcgcc gggaagctcg cacggcgagg tcgtcgatcc
     4621 cgacgcgtac gcgatgagct tctggtacga cgcgtcgctt gtcgtcctgc tcaccgagcc
     4681 atacgcgatc gacttcgttc gtcatcgttg tcgccaggtc gcacgcgagc tgtgcaacct
     4741 cttgccgctg ctcgatccgg atccagacgc gccggccgcg atcggcccgc gcccgcgccg
     4801 gccgacgcag cagtgagtct cgaccactgc ccgttctgca gcacgcaggc tgactggaag
     4861 ctgccgccgg ggacaacgcc gacggcgcgt tgatccggtg gacgcacgag ctacctgtgc
     4921 agccaggcgg accccagcga gagtacgtcc gctcgtattt cagccaatcg agtcgtccgc
     4981 gccggaatcg cagcactgtc cacttcggca aatgtgcgtg atattagtaa gttgcgaaat
     5041 taacgaatgc agataatcgc ctaataaggc gaagttttgt atttattgat gcatgctgct
     5101 cagcccgcgc caaaccgtgg agctcttcca cctcgtgttc gtgcgcgcgc tgttttcgaa
     5161 tgccgcggac aagacgctgc tcgcgatcaa aggcggtatc aaccttcgct tcttctttca
     5221 aagcgtccgg ttctcggagg acctcgatct cgacgtcgcc aagatggcga agggcacact
     5281 cgaaaaccgt gtcgacaagc ttctcgtctc gccagccgtc acggcgccgc tgcgcagtcg
     5341 cggaatcacc ctaaaagagg tcagcaagcc gaagcaaacc gagactgttc agcggtggaa
     5401 aatcgcgctc gcgacagcag gcggagcatt cagcgagcac acgaagctcg agttctctcg
     5461 tcgcggtggc accgacgacg ctgatctcga tcctgtagat ccggcgatcg cgaaggcgta
     5521 cgcgttgccc agcttcctcg caactcatta ccgctgcgcg gaagcagttc ggcaaaaaat
     5581 tggcgcgctc gccggccgca cgcagacgca agcccgggac atcttcgatc tacacgtgct
     5641 gtttgcccgg cccgatgctc ccgaagacct cgaagaacaa gagccgggtt ggttcgctaa
     5701 agctgtcgag aacgccggcc atgtcagcta cgaacaatac aagtccaccg tgatcgcatt
     5761 tctcgatcca gatcacgcag cgttgtactc agcggaggag acctgggatg cgatgcagct
     5821 aggtgtcatc gaaagcatcg agaagctgcg atgaacgcaa gcgccgcata cgcacagctc
     5881 gtggctctcg gtttaccaac actcgagacg gctgatgtcg cgtcaaagtt tcgcatctcg
     5941 acgaaggcgg cgaacaagct gctcggccgt ctctcgcgcg cgggtctgat catgcgcctt
     6001 cgggccgggc tgtggctcac ctcgcgcaca ccgccgagcg cgtattcatt ggtagagccg
     6061 ctcgctgcgc cgttcggtgc atacgtctct ctccacagcg cgctctatca ccacggcatg
     6121 atcgaacaga ttccggcggt ggtgtacgcc gtttctcttg ggcgcacccg acgcatacgc
     6181 actccggttg caacgttctc ctttcatcac atcgcgcccg agctgttcga cggattcaag
     6241 acgatgtcag gtgacgtgaa gctggccacc cccgagaaag cactgttcga cgtggcgtac
     6301 ttctcgggtg gacgttcgcg cctattcgcg catcttccgg agctcgagtt gccaaagcgg
     6361 ttcagccgtt ctgcgctcga tcgttgggtc gctcgaatcc ctgcggcgcg ccgccgttcg
     6421 atgacggcga gtcgcatcca gtcactgcta acgcctcgct aaggcctgct tcgcagtcgc
     6481 gcgaagtgcg cgaggagctc ggcgtcattc gcgccgtggt cgatcgcatc gcgcgtcgcg
     6541 tccagctcgt gctcgagcgc gtcgaggcgt acgaggtgcg cgtgcgcgcg cacgaggcgt
     6601 ggcatcgcga acgcgtcgag cgtcggcgat ttctcgatga acagctcgag cgacgtcttg
     6661 gcgttgctac acgccatctg ccgcgagctg atcaccggtt cgacaacggt cgtcagcgtc
     6721 tcggagagtc gtttgcgatc ggtggcgaga tagaagccga acttcgacat cgccattgcc
     6781 atcgccggcg ccagcgactc gtcaacgacg ctgtcgcaca gcttggccgc cttgaacgct
     6841 tcggcgcgca gctcgggcag gcgctcggcc gcgcgctctt cctgttgatg ttggtagcgc
     6901 cagcggagca cgacggtcac cgcgccgaac agcaccagcg cgatcgcaac cccgagcaac
     6961 gcagacttga tcacgagccg ctgacgagcg acttcagcac gctcgcgtca ccctcgagcg
     7021 cgcagcgctg catatagaag tgcatgccgc gctcgccgcc gagctcctcg ccaccgcctg
     7081 cgcggccggg gccgccgtgg agcaacgacg gcagcactgt ccctgggccg ggcgactgcg
     7141 acgccatctt cgacgagccg aggtaaagcc ggccggccca cgacgaggcg gcgctgacga
     7201 acgtcgcgac ccagtcgcga tcgtccgagt acgcgctgct gacgaggcaa ccttcgccgc
     7261 gtgcgacgaa ctccgccgcg aacacggccg tcccgttgta cgagccaacc gtcgacaccg
     7321 ggccaaacac ttcgtactcg ttcagcggcg cgcactccat cggcgacgtc gtcgagcgca
     7381 cgaccgggcc gacgaagtag cccttgccgg cgggcacgcc gaccggcgtc acttcgccgt
     7441 tgccgccatg cacgctctcg gtcgcggcgg cgagccgcgc gatgcctgtc ttgacgtcgt
     7501 cgcgctgttg cgcggtcgcg agtggtccca tgcgaacgtc ttcgcgggac ggatcaccga
     7561 tcgcgatcgc ggcgacgcgc tcacgaatcg cgtcgcacac gtccttgagc ttcacctcgg
     7621 gcacgagcac gcggcggatg gccgtgcact tctggccggt cttctgcgtc atgtcgcgca
     7681 ccacgtcggc gaggaacagg ttgccggctt cgctgctgag gtcgacgtct ggcccgagga
     7741 tcgccgcgtt gagcgagtcg gcctcgacgt tgagccgcac gccatgctcg acgatcttcg
     7801 gatgcgtgcg cagcatgcgg gcggtcgcgc tgccgccggt aaatgcgacg acgtctcccg
     7861 cactcaggtg atcgagcaac gtgccggcgg ggccgatcaa catctgcagc acacccttgg
     7921 gcagcagcgg ctcgaacagc tgcgcgagcc ggtgcgcgac cagcgcggtc gcggtcgccg
     7981 gcttgctgat caccggaacg ccggcgagca cggcgcatgc gagcttctcg cacgtacccc
     8041 aggccgggaa gttgaacgca ttgatgtgga ccgcaagacc gcggctgcgc gaccacacat
     8101 gctggccacc catccgcgcc gtgcgaccga cctggatcgg ctcaccgtcg ccgagcatcg
     8161 tcgcgttgcc cagcttggcg ccgagctcgg cgtagtgcga cagcgtgacg gcgccgccgt
     8221 cgatatcgaa cttggcgtcg ctccgcgtgt taccgccgtt ggcgaccgcg agtgcgatca
     8281 gctcctcgcg cgccccgtgg atcgccttgg cgagggagcc gagcagggcg ccacgttgtg
     8341 caaacgtcaa cagcccgagc gcgggggcac cctcggaccg ggcgtgtgcg aacgccgcgg
     8401 cgaggtcatg tccacccgtc gccacctcgg cgagcacctc ctcggtcgcc ggattgacca
     8461 gcggggagcg cgatcccgaa ccggaagacc aggtgccgtg caggtagcta gcgaggagct
     8521 gcatggcggg gggtttacca ggtttgttat tgtttcgggc aggggatgtc ctcgtcactg
     8581 cagaaacgcc tgctcgcagg cctgctcttt ttggccgtaa ccgtggtcgg cggtgccgct
     8641 ggcttctacg cgattggtgc tcgcgccggc gccgactgga cgttctggga ctgcctctac
     8701 atgaccgtca tcacggtcac gacggtcggg ttcggcgaaa cgctgaacca catggacgcg
     8761 gtcgagtacg cacgtggctt cacgatgctg ctgctcctgt tcggcacggg cagcatcgtg
     8821 ttcttcgctt cgacgatcac cgccttcatc atcgaaggcg acctcaagaa cgtcctgttc
     8881 gcgaacaagc tcaagaaacg gatgaagcgc atgaaggatc acgtcgtcgt gtgtggggcc
     8941 ggctcgacgg gccggaacgt catcgaagag ctgatcaaga ccggcgtgcc ggtgatcgcg
     9001 atcgatgtca acgaggtcga gctgaaggag atcgcggaca ggaacccgaa ggccgagtac
     9061 gcgtacatcg tcggtgacgc gaccgacgac gacgtgatcg cgcaggtcaa cctcaagggc
     9121 gcgcgtggcc tggtcgccgc actctcctcg gacaaggaca acctttacct cacggtctcc
     9181 gcacgccagg acaacccgac ggcacgcatc gtcgcccgct gcgccgagct ctcgcacgtc
     9241 gacaagatca agaagtccgg cgccgacgcg gtcgtgtcgc cgaactacat cggcggcctg
     9301 cgcctggtct ccgaaatgct gcgcccgtcg gtcgtccgct tcctcgacga gatgctgcgc
     9361 gatcgcgtcg cctatcgcat cgaagagatc acgctcggtg acagcgcggc cgagctcggc
     9421 tccacgctgc gcgacgcacg cgtccgcgag cgttacggca tgaccgtgct cgcggtgcgc
     9481 aggcgggaag gcgagggctg gacgtacaac ccggatgctg cggagaagat cggcccaggc
     9541 atgacgctcg tcgtcctcgg ctcaccggaa caggtgagcc agctcaaaga cgcgacgaag
     9601 tagtgctctc gagagcatga atctgacgat cggcgcgtgc catccgaacc gcacgcacga
     9661 tcaccatcgc cgccgcaccc gcgcactgcg tgagcatcag cagccacagc acgagatcga
     9721 ggcggtcgat caacagcccg agacagagcc agaaccacag atcggcctct tcgaacacga
     9781 aaacgaccgc gaccttcttc gcaaaccaga tcgcccaatc gcgcgcgctg cgctcgggcg
     9841 gcggcttgat cacgatcggc gcggtgtacc tggcgacggt acctgcggga tcctgcttcg
     9901 cttcgagcca gcgcagtcgc tcggtctcgg cctggcagag ccatttcatg tagccgcgca
     9961 gctcgagcgc ggcgacggcg gcacccgcga acacgaggta catcgcgtcc gccgacgtgc
    10021 gataggcctg ccagccgagc accatcacga tcaccgccca cgtgatcatg tcggacacct
    10081 tgtcgtagta agagccgagc ttcgtgaacg tgcggcgata gcgggcaagc gtgccgtcga
    10141 ggtggtcgaa gatcaggccg agctggagca gcacgacaca gagcacccaa tgctccggcc
    10201 gcaggagcaa ccacgcgccg gctagcttgc acaggttcga cagcgtggtg agccggttcg
    10261 cggtgacaaa ccgccagtcg gcgatgacca acatgatcgc gatcgagatc ggccggatca
    10321 cgagcgcggg ccaccagtcc tcgctttgga agttgcggat gcgctgcagc cgctcgcgat
    10381 tcatgcggcg cgcatcctag actagttcgg ccggggctag ccgctgttct cgcgttccgt
    10441 cgatctcttg gtcagggcag acaacgctac atcgggaggc gccacgaact cgcgatcctg
    10501 gccgcgccac tggtcccagc ggctcgcggt gcggacgcaa ccaacggtgc acgtcggtgc
    10561 gcaggacttc ttcgtgtaga actgctcgcg taggtcctcg aacgtgtagt cgagcagagg
    10621 cttcgtgaac acgccgcgct gttgcgagca ccagtgcacg acgccgtgct cgtcgacata
    10681 gaggtagcgc gcgccggctc ggcacttgaa gtctgcgcgg ccggtctcga tgaggcgtgc
    10741 gcggtagtcc tgtgactcgt tgaagcgctt gccgacgatc tcgctgactt cctgcatcag
    10801 ctgcttgccg cgctcgtcga gaatcatgcc gccatcacca tcgtggatga tcgacgcgcg
    10861 aggacgaaag ccggcgtctc gcgcgaactt catcacttcg agcacttctt catcgttgcc
    10921 ggcaccgagc acggcgttga tctggaccgt gaacttcgcg tggttctgca gatgctcgat
    10981 tttcttccgc agtgccttga gcaccttcac tgtcgtgtcg ttcggcgtga cgccgtcgat
    11041 actgatctgg aggtgcgtga gcccggcgtc attgagctgc ttcacgattt cggcagacaa
    11101 cagataaccg ttcgtgatga tccagcgctc gttgaagttg tacgaggcag cgtaggcaac
    11161 gagctcgatg agatcagggt ggagcagcgt ttctccaccg gtgtactcgc agaacagcgt
    11221 ccccaggctc gcgagcttgt cgacacgctg cttgagaacg tcggtaggca ccggcggcga
    11281 gaacgtgtcg tactcgttgc agtacccgca cgttagattg cagcgccgcg tgatcacgag
    11341 ctgcgcgagg aggggcccgt aggcaagcgc gcggggcgcg aacctccata accgcttccg
    11401 caccacatcc gttcgctcca tctgggcgcg gtgttacacg agtcgtacgc gttgaacaag
    11461 gaacttcctc gacaggctgc aagtgatgcg tgctagggtc cgcgccctat gagcggcacg
    11521 ttccaccgcg tgtttcgagc gatcacacgc agctacccgt atacgacgcc ggcgtacgag
    11581 atcaacgtag cgcttgcgcc actgctcgat cgcgagactc cgctgtggct cgaggcaaac
    11641 ggctaccgtg gcagcccgcg catgatgctg aacgttcgta atcgatttca gcgcaagatc
    11701 ttctactttc cggtcgcgta cgcgcggcac tggctccaag caccactcgg cgagtacatg
    11761 cgccaacgct tgcgacccgg cgattggttc gtcgatatcg gcgcgaacat tggctactac
    11821 agcttgctcg cagccggctt ggtcggtgcc gaaggaacgg tgatgtcgtt cgagcccgag
    11881 cccggggcgt tcgaatcgct gactcgcacg atggcactca acgaatgcgg tcaggtccag
    11941 ccgcacaacg ttggcttgtc caatcgcgag ggcaaactgc agctgtatcg cgcgcgcgat
    12001 acgtcgcatt cgttggtcga ggcgacggag aagctgggca atttccgcgg tacggtcgat
    12061 gttgacgtgt atccactcga tcgcttccca ccggctgccg cgctcgacgg ccgcgtgcgg
    12121 ctgatcaagg tcgatgtcga aggtgaggag gctcgcgtcg cgcagggcat gctcgagacg
    12181 ctcgcgcgca tcggtaagcc gcccgtgtgg agcgaggttc gcggccccga agggtccgag
    12241 cgagcgcccg atacgttcgc accgatgctc gaagcgttgt cccagctcgg ttatcgcgca
    12301 tttcgctggc gcaaagacgg gatcacggaa gtcacacaac acgacgtgcg taagcgggag
    12361 gacatcctgt tcgccgtcga ccccaacgca tgatcgagca agcagtcatt cttgcggctg
    12421 gcaaaggcac gcgacttcgc gagaccgccg acgcgttgcc caagccgctc acaaccgtcg
    12481 gcggagtccc gctgatcaaa cgtacgattc tgacgctcgc agacgcggga gtgcggcgcg
    12541 tcgtggtcgt cacgggcttc atggccgacc aggtccgcgc gaccgtgtcc ggcgatccga
    12601 cgtacgcagc gcgcgggctc gaaatcgagt tcgttcataa cgccgagttc gagctgtcga
    12661 atggcatctc ggtgatgctc ggaggcaagg ccgccggtgg cgcgttcgtg ctttcgatgg
    12721 ctgaccacat ctatacggtc gcgatcgcgc ggttgctcgt tggctgcaac ctcgacactg
    12781 ccgatctggt tctcgcgact gatccgcgtg tcgaccaggt gctcgacatc gacgacgcga
    12841 cgaaagtgcg cacccaagac ggtcgcattg tcgagatcgg caagcagatc gccacctacg
    12901 accgcatcga ttgcggtgtg ttcgcagttg gcccgcggct catcgatgcg ctcgccgagg
    12961 agcgcgctgc gcggggggat tgctcgctct ccgacggcgt acgcaggctt gcgcaagccg
    13021 gtcgagcacg cgttatcgac atcggcaacg agccctggca agacgtcgat acgccggcag
    13081 atcgcgaaca cgcggaacgt gcccttggca aatctcggta agcggcgtcc gcatggctga
    13141 gatctgaacg acgctgtagg ctgtacgctg ctcgatttcg atgacccctc aacggatgtc
    13201 tacacgcgtc aaccgtgggt tggcgtggct cggcttcgcg agcacagcgg tgggattgct
    13261 cgacatcgtc gcgatgttgc tgatcctcaa cttctggatc tccaaggcgg accttgggat
    13321 cgcgacgatg gctgtgtgga tttttcccat cttggaccag gcgacggagc tcgggctatc
    13381 cgcggcactg atccagcgcg acgatcacac cgagacgcgc acctcgacgg tgttctgggt
    13441 gaatctcatg ttgtcgctgg ccgcgtttct gctgctcgtc gcgggtgcgc cgccactcgc
    13501 caagcacttc tatcatcagg cgatcgtcgg ctggatgttg atcgcgtacg gctcgaagct
    13561 gctgctccag aacgcgtaca tcatcccaac agcgatgatg aagcgcgagc tccgattcaa
    13621 ggagctttcg atcatcaagc tgcttgcaaa cattgccgag ttctttggca aggtcggctt
    13681 cgcgtgggcg ggattcggca tttggtgcta cgtcctcggt ccgctcgcac gcacgctcgt
    13741 ctatgcggtc ggtttgcagt ggtgcagccc gtaccgcccg cgcatgtcgt tcaacctcgc
    13801 cgatgcccgc gactacgtcc gcttcggctt gcgcacgtcc ggcagccaga tcctgttcta
    13861 cttctacacg aacattgact acccgatcgt cggcgtcttc ttcgggaaag aggcgctcgg
    13921 cctctaccgc tacgcgtacg agatcgtgct cgagccggtg cgcatcctgt cgaacgtcgt
    13981 tgtcgatatc gcgttcccgg cattcgcgcg actgcgccac tcgaaggagc agctcgtcgc
    14041 gcagttcgtt tcgttcacga agctcaacct catcacggtg atgtcctatg cggccgtcgt
    14101 gttcgttgcg gcgccggagc tcatcgccgt gctgttccca gattacgccg gcgccgaaac
    14161 cgcggcgcgc atcctgtgcg gtgtcgctgt gctgcgcgcg gtcggctttg taatgccgcc
    14221 gttgctcgat ggcgtcggtc gtccggaacg gacgttcacg tacatgacat gcgcagcagt
    14281 gacgatgccg atcctcttcg tcatcggtgc ggcgatcggc gtacgcaccc acggcggatt
    14341 cgagtcggtc gcggtcgcat gggccgttgg ttatccgatc gcgttctcgg ttcttgcgta
    14401 catggccacg cagacgatcg gctggtccgc gctcaaatac ctgcgcgcgg tgattggcgt
    14461 ggtcgcgtgc ttggtcgtcg ccggcgctat ggcggctgca ctgcgctacg cgctcgcggg
    14521 ttactcgtcg ggcattaagc tactcgcgac cgccggtacc gtcatcctcg cgtcgggatt
    14581 cctactcgcg tacacgcagg gactcacgtt gcgctcggcg atgcgctcga tgcgcgagcc
    14641 gcccaccgat ccgtccttgt gatatttcgg gcagtcggca tatggtccgc gcatggcaag
    14701 gatcgggttg tggcggtacc tgtacgcacc atttcacgta cagatggttg tgatccggcg
    14761 gtgcaacctt tcatgtggct actgcaacga gttcgatgat cattcgccgc cggttccgtt
    14821 cgaggaactg tgccggcgga tcgacaagat ctacgagctc ggtgcatggg cgatcgagct
    14881 gaccggtggc gagccgatgg agcatcctca gctcgtcgat ctggttcgct acgcgaagca
    14941 gaagggcttc tacaaggttc agctgatctc caacgcgtac ctgtgga
//
LOCUS       DASUFX010000580        14943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2040114, whole genome shotgun sequence.
ACCESSION   DASUFX010000580 DASUFX010000000
VERSION     DASUFX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040114"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..2274
                     /locus_tag="VFV99_23360"
     CDS             76..2274
                     /locus_tag="VFV99_23360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5062337.1"
                     /translation="MKQLLAELVDVLGHDNDLLRTRMRVDSDSELQEREFLRNDIIHR
                     LESGHLEAPCRELQLLSDWVWAAEHQRLSALNKQHSALLDLCPDAAAIVDLDGRIQYV
                     NRAATLLIHAMTGLVDEHIIGKTARELGIVIEAGPSPADLPALARSNSVAEALLAGHW
                     HEIRATAVRGKDGRANALGLTFTDIHERKIGLVRLELLSKLSRLVGTVDRDQLWTALS
                     YVAIPQLADWCVVSVVDEREIKSTFISHRDPSKASLREKLVNLAIGPRHHPLWQNLLS
                     SGFQLLADVTDDMVRKLLESAHADLVSQVGIRSLMVVPVVSRGKLIAIITLAYTNESG
                     RRYGRDDPGVALELALHAAHIAENARLVAETKASDARFRIALAEARTIVYEQDASLRY
                     VFDYGHELSASMLGKRAADALPPDEAAALDKIKQHVLDTGEPVHTEIELSFGGEKRWY
                     RETIEAVRDRRGRRVGVIGAATDLTEEKAAQAALQQALAIRDRVMGILSHDLRNPLST
                     MTMAASMLLHTDDASGTVKNTAMMIERAANRMTEMIETLLDFTRVRVTGKPLSIILSH
                     CNLAEIVNEIVDEARNAAPGRTIALDVRGCTTGVCDASRIGQALSNLTANALTYGDAT
                     QPISIELIGTDRELLLRVHNEGRAIPEALQRVMFEPFARGEEDVVSPHGLGLGLFVVR
                     EIVASHNGSIAVESTPEAGTTFTIRLPTIPPDAEAAGTIVDTHTEPPAVH"
     gene            complement(2275..2736)
                     /locus_tag="VFV99_23365"
     CDS             complement(2275..2736)
                     /locus_tag="VFV99_23365"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX5062338.1"
                     /translation="MHRSLRRLFKGVVLGLAFFVGFVGVLQLVPYGRAHSNPPVVQEP
                     RWDQPRTRELAKRACFDCHSNETRWPWYSHLAPFSWTLQRDVEVGRSVLNFSEWNRSY
                     ELAHEAGPTVIRRDMPPSKYTLMHGDADLTEDEKVELARGLHATFGAPGRI"
     gene            2829..3296
                     /locus_tag="VFV99_23370"
     CDS             2829..3296
                     /locus_tag="VFV99_23370"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5062339.1"
                     /translation="MSEHHATITWSTAVPVADFAKGKYSREHEWAFDGGTTVQASSSP
                     SVVPLPWSSQGAVDPEEAFVASISSCHMLTFLYLAMKKGFALTSYRDAAVGTMTKGAN
                     GVPWISRVTLRPELGWAGDKRPSAAELADLHHHAHEQCFISNSIKTEVVVEGV"
     gene            3395..4345
                     /locus_tag="VFV99_23375"
     CDS             3395..4345
                     /locus_tag="VFV99_23375"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5062340.1"
                     /translation="MTGATGVIGRRVIPQCVQRGHQVTAIARSPEGLAAIERLGATAI
                     VSDLFDRDRLATAMPGHDVVINLATHMPSSTIRMFLPGAWRENDRVRREGSATLVAAA
                     LAAGVGRFIQESFAMIYRDNGDAWIDESSPIEPAAYNRTVVDAERAAQRFTEAGRIGI
                     VLRFGAFYGPDGRFFADMVRMVNRGFSPLLGPADAYISSVSHDDAAAAVIAALGLPAG
                     IYNVVDDEPLTHRECMNSLADALGVHHPRILPTWLTYFGGTPARVLARSLRMSNRKLE
                     ASSAWKPKFRSVREGWPSAIAPFIAEPRPPAHVAPTAPAR"
     gene            complement(4394..6148)
                     /locus_tag="VFV99_23380"
     CDS             complement(4394..6148)
                     /locus_tag="VFV99_23380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5062341.1"
                     /translation="MTVGDGPGFAFKDPARRKKLEAAFPAIDKIFDDELKAQGIPGIA
                     LGIVIDGELAYAKGYGIVNVETKAVPDADTVYRIGSISKSFTALALLSLRDDGALDLD
                     DPLAKWIPEANGLVYPTSDSPRITLRQLSNHTSGLPRMGTFDFEATPSDKIVTESLKG
                     LPLESAPGTRWNYSNLGFGLLGMVVGRAAKSNLHDVVGARIWTPLGMTATAWDPEQVP
                     AAKLAPAYMPGPKGPMNNPKPARLGVVDGAGGIYSSVRDMAKYVAFQMSAYPPRSDAD
                     GGKIKRATLREAHSTGVPAGFKYDPPAMAMSYGFGWSQFATCEVDDLVTHNGAIDSYR
                     SEIRFSPSRGVGLVVLTNFGNGNPAPFADKTMSELIKTGAAEPRAVQPSKGLSDAMEK
                     MLTVYNQWDEKKLASYLSRPVDPREHDELATYFKLHGACTTFEPSKIVSATAGGFRLK
                     CERGTFEMELNINGKGLIDGFIGTSTGIQAPPEIAKAGADIVSLHTKWDDKIFKKYLA
                     NMPVPAAQMKKLAEQMHARHGDCKIAGAMHQGFDWGFALTCKKENVELWTPSAPDNPT
                     QFVGLQLRPPRDEAKHCK"
     gene            complement(6368..7183)
                     /locus_tag="VFV99_23385"
     CDS             complement(6368..7183)
                     /locus_tag="VFV99_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062342.1"
                     /translation="MAHDGIESCEVPGSYPDGGEADAYVDGDRDQDTVVDSIDNCPDV
                     ANTNQADDDGDGTGDVCDPCPPFADNSDGDGDGVGNACDPNPATAGDTLVAFEGFTGA
                     LPTTWTSSGTAMINSGEMSLLAGDSATSLLTMPSPSAARVEVRASVVIDLITASGLNL
                     GSVNLIDRLEPNTDKSVACQLAGLADGTQELLRVFDASTATEINSAAHALTPGDPIEL
                     RLRRSGTSYACYASDPSLEVVGSAAFLPAAPRIGLRVRGAAARFRWVMVVTSP"
     gene            complement(7402..7698)
                     /locus_tag="VFV99_23390"
     CDS             complement(7402..7698)
                     /locus_tag="VFV99_23390"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5062343.1"
                     /translation="MPMYVTLAKLTDQGIRNYKDTVTRAEKWWAAIKEAGGRLIQEVW
                     TTGDYDVVALFEAPDDETAASLSLQVSSLGNVRTTTSRAFSAEEVKKIIAKNPR"
     gene            7828..9132
                     /locus_tag="VFV99_23395"
     CDS             7828..9132
                     /locus_tag="VFV99_23395"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HEX5062344.1"
                     /translation="MMRWVLVACVATACGGSSKHGPTVDPERARIEAMEPPSPYVTQS
                     VRGYTSPTACSQGPFRIEASALGAHFGEQLEVSICAPRSLQGDYQFTKGTDRDEVKHF
                     GDRNNSEKCLPTADELARYGAVSGGASGGGGTSVASGGGGATSQSSRATSVASATAAS
                     APVQLAEVSGTVAEKCPHGTYLSGILYYTIVSSGDHEAWKQGMSLVLDVWSTEPLDLT
                     GAVFVVIQRGVRADMTADAWKKYNEDWDRWNKLWNAYLDGEVTAGRATLVDNTARSSA
                     APPPARTEVKPPKPSVHAEWIAGFWQLRGEWIWSAGFWRVPESDIVAEQTVEAPIAPP
                     PPKAEAPPPPPAPPASPPPAPTVTASATVTVTPPAPAATANLVWTPGYWAWNGTAYIW
                     IEGAWRIPPQANARWVAPTWQPRRGRVVMVPGGWSVSVHIGR"
     gene            9161..10459
                     /locus_tag="VFV99_23400"
     CDS             9161..10459
                     /locus_tag="VFV99_23400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5062345.1"
                     /translation="MLRAALVLSVVLFASPLHAAPTLIKAAHVLDGVSAKAKDNVAIL
                     VDGDRITAVGPAAELAKKAPNAKVIDLGNATVLPGLIDAHTHVFLDDDVGPGVYDAIL
                     LKQSVPYRAIAAAANARKALHYGFTTLRDLETEGAMYADVDVKTAIIRGITEGPRMQV
                     ATRAFAPTGMYPLKGYNWELTMPIGVQTIDGVDEARKAVREQVAHGADWIKFYADHGV
                     YEGKPDKPVRSIVNFTAAEAAAIVDEAHRLGVKISAHANGWDGIDAALRAGVDSIEHG
                     QGLTDDLLVRMVKQRVAWCPTLLAYRYSEQKLGKNARSFMMAPHARALAAAHKRGVQI
                     VFGTDAGAFPWTMNPAAEAKLMVDAGMPAADVVRAMTSAAATLLDPFCRAGAKQCASS
                     DVGVIAVGKLADLVAVAGDPLEDITQLEHVKFVMKAGVTY"
     gene            10533..11405
                     /locus_tag="VFV99_23405"
     CDS             10533..11405
                     /locus_tag="VFV99_23405"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX5062346.1"
                     /translation="MRLVVCGLCIVAAGCGRLGFGERTGGGTGDGGGGSDDGQLDDGG
                     GSGSVDGPMGDGPGTDAFVCVTGPNHDEDGDGVRDACDVCPHIPDSAQADTDGDRVGD
                     ACDPEPNNARQSILLFDPFVTIGASWTNGGATQGTDEIIIDARGGKAFRITRSITPTH
                     DLFMIGATTGAGDASTHHISLVTFPNTGNADAYCEMYDNGSSTATQFTWTFDNMTYMH
                     GAPSSWGATRLANGSGTFSYDLDVSQVTCKSTWQSNQRISTAARPAIAAEKFMIYGEN
                     LLMRVQYIIIIRTN"
     gene            11405..11959
                     /locus_tag="VFV99_23410"
     CDS             11405..11959
                     /locus_tag="VFV99_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062347.1"
                     /translation="MDTLTIARQLADFHPSARLPCPACGVSVNGDNLAKHLAKVHADF
                     SSLTPPWRGRGWFGLLPASIAFDGDAIVLRRLGQRRVSLPCKLEVGTLVGTRPDAISA
                     NQSVDYNTPGETVRVGRYLRLVGDRSITIGCRSATQFHAHWRREQLIDGGRRRSCHVM
                     LSRPALAAIEYELARRGMLVPQSQ"
     gene            complement(11962..12315)
                     /locus_tag="VFV99_23415"
     CDS             complement(11962..12315)
                     /locus_tag="VFV99_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062348.1"
                     /translation="MPPAEKVFELEGLVAEVVGARELGAGMMTLELALSPWRVRELGD
                     AEIRRARVSIEMDLDMADAWLRVLDVGDCVRVVCASVSEGADELGRPCFHVKGTSVDQ
                     CPPDCVSDWQTLAPR"
     gene            complement(12302..12991)
                     /gene="yfcF"
                     /locus_tag="VFV99_23420"
     CDS             complement(12302..12991)
                     /gene="yfcF"
                     /locus_tag="VFV99_23420"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF011693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEX5062349.1"
                     /translation="MTDSHNASRERTLTLYVDGYFVNQWDASCVTALEEKQLVYSKAR
                     ALLRDGGGVPPALVSRTNVSRVPALQHGDVWLAESSAIIEYLEEVFPPPAYPRLLPAD
                     TYARAKARQLMAFVRADVWALRTERSWWMCVYPEPQPPPMSRACEREARELVALTERL
                     LAAGDLDPAHWNMAHADLALTLLRLDRTSYPLPDRVKAFLDSAVSRPSMRVYLEHPRP
                     PFRPHDAYAAG"
     gene            complement(13049..13297)
                     /locus_tag="VFV99_23425"
     CDS             complement(13049..13297)
                     /locus_tag="VFV99_23425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062350.1"
                     /translation="MIAIVDFDALTEADIQALLNVRGDRWTGALIALGRVESDLRQVL
                     GVRETVLRPFGSERLRKAMSEIRVSKATESLPAIRAAG"
     gene            complement(13620..14126)
                     /locus_tag="VFV99_23430"
     CDS             complement(13620..14126)
                     /locus_tag="VFV99_23430"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5062351.1"
                     /translation="MRSKAKPKKATSRTSAPRKSPAKAKAKPVVLLSGGNPQIAMADG
                     DAPVQAYIAAMPDWKRALGKRLDALIVRSVPNVCKAVKWNSPLYGLEGQGWFLSFHVF
                     ARYVKLTFFHGTALRPLPPGPSKNKGTRYLDIHEDDTLDEAQLTTWIKQAAALPGWIP
                     GQPFEAIE"
     gene            complement(14126..14542)
                     /locus_tag="VFV99_23435"
     CDS             complement(14126..14542)
                     /locus_tag="VFV99_23435"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5062352.1"
                     /translation="MNPSERIDQLIAGLSDWRGKTLAKVRKIILDVDREIIEEWKWMG
                     SPVWSRDGMIAVGNAHKDKVKLTFSNGARLPDPDKLFNAGLDGGQWRALDLFEGDKVD
                     ERALENMVRAAIVFNQSKVKKKAPAKARAKAAKSKK"
     gene            complement(14552..>14943)
                     /locus_tag="VFV99_23440"
     CDS             complement(14552..>14943)
                     /locus_tag="VFV99_23440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5062353.1"
                     /translation="LNIKLAHVYVDDQEKCLRFYADVLGFAKKDDETNGSFRWLTVTA
                     PGDGEGTALLLALNSDPAAKAYQEAMFKQSQPAAMFYTDDVKGEYERIKAKGAEFTMP
                     PTDVTFATIAMLKDTCGNIIQITQLSR"
BASE COUNT         2611 a         5044 c         4697 g         2591 t
ORIGIN      
        1 atcgcacgtg aggtcgccac ggctacgacc acgtccgagc aaggtgcgga atcgagggct
       61 gcgcgaagcg agcgcttgaa gcagctcctc gccgagttag tcgacgttct tggccacgac
      121 aacgacttgc tgaggacccg catgcgcgtc gacagcgaca gcgagcttca ggagcgcgag
      181 tttctgcgca atgacatcat ccatcggctc gagtctggtc acctcgaagc gccctgccga
      241 gagttgcaac tcctctctga ctgggtgtgg gcagcggaac atcagcggct cagcgcgctg
      301 aacaagcagc actctgcatt gctcgatctc tgtccggacg ccgccgcgat cgtcgacctc
      361 gacggccgca tccagtacgt gaatcgagcc gcgacgctgc tgatccacgc gatgacgggg
      421 ctcgtcgacg aacacatcat cggcaagacg gcacgcgagc tcggcatcgt gatcgaggca
      481 ggtccgtcac ctgccgacct tccggctctc gcacgctcga attcggtcgc cgaggcgttg
      541 ctcgcggggc attggcacga gatccgagcg acggcggtcc gcggaaagga cggtcgcgcg
      601 aacgcgctcg ggctcacctt caccgatatt cacgagcgca agatcgggct ggtccggctc
      661 gagctcctct cgaagctgag ccgcctcgtc ggcaccgtcg atcgcgacca gctctggacg
      721 gcgctcagtt acgtggcgat cccccagctc gccgactggt gtgtggtcag tgtcgtcgat
      781 gagcgcgaga tcaagtccac gttcatttcg catcgcgacc cgtccaaagc gtcactgcgc
      841 gagaagctcg tcaatctcgc catcggtccg cgacatcacc cgctctggca aaacttgctc
      901 tcgagtggct ttcagctgct cgccgacgtc accgatgaca tggtccgaaa gctgctcgaa
      961 agcgcacacg ccgatctcgt ctcccaggtc ggcattcggt cgctgatggt cgtcccggtc
     1021 gtctcgcgcg gcaagctgat cgcgatcatc acgctcgcat acaccaacga gtcgggacga
     1081 cgttacggac gagatgaccc gggcgtcgcg ctcgagctcg cacttcatgc ggcccacatc
     1141 gccgagaacg cacgcctcgt cgccgaaacc aaagccagcg acgcgcgctt tcggatcgcc
     1201 ctcgccgaag ctcgcacgat cgtctacgag caagacgcgt cactccgcta cgtcttcgat
     1261 tacggtcacg agctctcggc gtcgatgcta ggcaagcgtg cggcggacgc actgccgccc
     1321 gacgaagcag cagcgttaga caagatcaag cagcacgtcc tcgacacggg cgagccggtt
     1381 cacaccgaga tcgagctttc gttcggtggc gaaaagcgct ggtaccgcga gacgatcgaa
     1441 gcagtccgtg atcggcgggg tcgacgagtc ggcgtgatcg gagcggcgac ggacttaacc
     1501 gaagagaagg ccgcgcaagc agcacttcaa caagccctcg cgattcgcga ccgcgtcatg
     1561 ggaatcctct cgcacgacct tcgcaatccg ctcagcacga tgacgatggc tgcatcgatg
     1621 cttctccata ccgacgatgc ttctggcacc gtgaagaaca cggcaatgat gatcgagcgc
     1681 gccgccaatc gcatgaccga gatgatcgag acgttgctcg acttcacgcg ggtgcgtgtc
     1741 accggcaagc cgctgtccat catcctcagc cactgcaacc ttgccgagat cgtgaacgag
     1801 atcgtcgacg aagctcgcaa cgccgccccc ggccgaacca tcgcgctgga cgttcgcggc
     1861 tgcacgacgg gggtgtgcga tgcatcgcga attggccagg cgctctcgaa tctgacagcg
     1921 aacgcgctca cctatggcga tgcgacgcaa cccatctcga tcgagctcat cgggaccgat
     1981 cgtgagctct tgctgcgtgt ccacaacgag ggccgcgcaa ttcccgaggc gctacagcgt
     2041 gtgatgttcg aaccgttcgc gcgaggcgaa gaggatgtcg tctcaccgca cggcctcggc
     2101 ctcggtctgt tcgtcgtgcg agagatcgtc gcgtcacaca acggttcgat cgcagtcgag
     2161 tccacgcccg aggcaggcac cacgttcacg atccgactgc cgactattcc gcctgatgcc
     2221 gaggcggccg gcacgatcgt cgacacgcat accgagcctc ctgccgtgca ctgatcagat
     2281 cctgcccggc gcgccgaacg tcgcgtgtag accacgcgcg agctcgacct tctcgtcctc
     2341 ggtcagatcc gcgtcgccgt gcatgagcgt gtacttgctc ggcggcatgt cgcgccggat
     2401 gacggtgggt cctgcctcgt gcgcgagctc gtaggagcga ttccactccg agaagttcag
     2461 cacgctgcgc ccgacttcga catcgcgttg cagcgtccac gagaacggcg cgaggtgcga
     2521 gtaccacggc caccgggtct cgttgctatg gcagtcgaag cacgcgcgct tcgcgagctc
     2581 gcgcgtgcgt ggttgatccc aacgcggctc ttgcacgacc ggcgggttcg aatgcgcgcg
     2641 gccgtacggc accagctgca gcacaccgac gaagccaacg aagaacgcga gtcccagtac
     2701 gacacccttg aacaagcgac gtagtgagcg atgcatgcac cttcgatgcg gtggccaccg
     2761 ccccatggcc ggagcaattt gccgcagggc taagtggtca ggagttggcc gaatgctagg
     2821 gtccgcacat gtccgagcat cacgcgacaa ttacatggtc cactgctgtg cctgtcgccg
     2881 acttcgcgaa gggcaagtac tcgcgcgagc acgagtgggc gttcgatggc ggcacgaccg
     2941 tgcaggcctc gtcgtcgccg tccgtcgtgc cattgccgtg gtcgagccaa ggcgcggtcg
     3001 atccggaaga agcgttcgtg gcctcgatct cgagctgcca catgctgacg tttctgtacc
     3061 tcgccatgaa aaagggcttc gcgctcacga gctaccgcga cgccgcggtc ggcacgatga
     3121 cgaagggcgc gaatggcgtg ccgtggattt cgagggtcac attgcgtccc gagctcggat
     3181 gggctggcga caagcgaccc tccgccgccg agcttgccga cctgcatcat cacgcgcacg
     3241 agcagtgctt catctccaat tcgatcaaga ccgaggtcgt cgtcgagggc gtttagtgga
     3301 ctccgccgac ggctcacgtc gagacgccta cgagtgaaac cctgcgctgc gccgcggcac
     3361 gcgcattgct cgccgatcgg tcatgcgcat cttcatgaca ggagcgaccg gcgttattgg
     3421 ccgtcgcgtg attccgcaat gtgtgcagcg cggccatcag gtcacggcga ttgcgcgttc
     3481 accggaggga ctcgccgcga tcgagcggct cggcgctacc gcgatcgtct ctgatttgtt
     3541 cgaccgcgat cgactcgcaa cagcgatgcc gggccacgac gtggtgatca atcttgcgac
     3601 gcacatgccg tcgtcgacca tccggatgtt tctgcccggc gcatggcgcg agaacgatcg
     3661 cgttcgtcgt gaaggctctg cgacgctcgt ggcggcagca cttgccgctg gcgtcggccg
     3721 gttcatccag gagtcgttcg cgatgatcta tcgcgataac ggcgatgcgt ggattgacga
     3781 gtcatcgccg atcgagcccg ctgcctacaa ccgcacggtc gtcgacgcag aacgtgcagc
     3841 acagcgattc accgaggcag gacgcatcgg aatcgtactt cgcttcggcg cattctacgg
     3901 acccgacggc cgcttcttcg ctgacatggt gcgcatggtc aatcgcggat tctcaccgct
     3961 gcttggccca gccgacgcat acatctcgtc ggtctcccac gacgacgcgg cggccgccgt
     4021 gatcgcggcg ctcgggctgc cggcgggtat ctacaacgtc gtcgatgatg agccgctcac
     4081 gcatcgcgaa tgcatgaact cactcgccga tgcgctcggc gtccaccacc cacgaatctt
     4141 gccgacgtgg ctgacgtact tcggtggcac gcccgcgcgc gtactcgccc gttcgcttcg
     4201 catgtcgaac cgtaagctcg aagcatcgag cgcgtggaag ccgaagttcc ggtcggtgcg
     4261 cgaaggctgg ccaagcgcca tcgcgccgtt cattgccgag ccgcggccgc ctgcacacgt
     4321 ggcgccgacc gcgccggctc gctgacagca cgatcacgac gccgatcgcg cgccgatccg
     4381 gaggcgctgc gccctactta cagtgctttg cctcgtcgcg cggcgggcgc agttgcagac
     4441 cgacgaactg cgtcgggttg tcgggcgcac tcggcgtcca cagctcgacg ttctccttct
     4501 tacaggtgag cgcgaagccc cagtcgaacc cttggtgcat cgcccctgcg atcttgcagt
     4561 cgccgtgtct cgcatgcatc tgttccgcca gcttcttcat ctgcgcggcg ggcaccggca
     4621 tgttcgcgag gtacttcttg aagatcttgt cgtcccactt cgtgtgcagc gacacgatgt
     4681 ccgccccggc ctttgcgatc tccggcgggg cctggatgcc ggtcgacgtg ccgatgaagc
     4741 cgtcgatcaa gcccttgccg ttgatgttga gctccatctc gaacgtgccg cgctcgcact
     4801 tgaggcgaaa acctcccgcg gtcgccgaca cgatcttgct cggctcgaac gtggtgcacg
     4861 cgccatgcag tttgaaatac gtcgcgagct cgtcgtgctc gcgcggatcg accggacgcg
     4921 acagatacga tgcaagcttc ttctcgtccc attggttgta aaccgtgagc atcttctcca
     4981 tcgcgtcgct gagccccttg ctcggctgca ccgcccgcgg ttcggccgcg ccggtcttga
     5041 tcagctcgct cattgtcttg tcggcaaacg gagcgggatt gccattcccg aagttcgtca
     5101 gcacgacaag cccgacgcca cgcgacggcg agaagcgaat ctccgagcgg tagctgtcga
     5161 tcgcgccgtt gtgcgtgacg agatcgtcga cctcgcacgt cgcgaactga ctccagccaa
     5221 atccgtagct catcgccatc gccggcgggt cgtacttgaa tcccgccggc acgcctgtcg
     5281 agtgcgcttc gcgtagcgtc gcacgcttga tcttgccgcc atcggcgtcg ctgcgcgggg
     5341 gatacgcaga catctggaac gcgacgtact tcgccatgtc gcgcacggac gagtagattc
     5401 caccagcgcc gtcgacaacg ccgaggcgcg cgggcttcgg gttattcatc gggcccttcg
     5461 gtcccggcat gtatgcaggc gcgagcttgg ccgcaggcac ttgctcggga tcccaggccg
     5521 tcgcggtcat gccgagcggc gtccagatcc gcgcgccgac cacgtcgtgg aggttcgact
     5581 tcgccgcgcg cccgaccacc atgccgagca gaccgaaccc gaggttcgag tagttccacc
     5641 gcgtgccggg cgcgctctcg agcggcagcc ccttgagcga ctcggtcacg atcttgtcgg
     5701 acggcgtcgc ctcgaaatcg aacgtgccca tgcgtggcag gcccgacgtg tggttcgaga
     5761 gctggcgcaa cgtgatccgg ggcgagtcac tcgtcggata cacgagcccg tttgcttcgg
     5821 ggatccactt cgctagcgga tcatcgagat ccagcgcgcc gtcatcgcgc agcgacagca
     5881 gcgcgagggc cgtgaacgac ttcgagatcg agccgatgcg atacaccgtg tcggcgtccg
     5941 gcacggcctt cgtctcgacg ttcacgatgc cgtagccctt cgcgtacgcg agctcgccgt
     6001 cgatcacgat gccgagtgcg atgcccggaa tcccttgggc cttcagctcg tcgtcgaaga
     6061 tcttgtcgat cgcgggaaac gccgcttcga gcttcttccg gcgtgcaggg tccttgaacg
     6121 cgaagcctgg cccgtcgccg acggtcattg cgggcagcgc agtagcggtt gccgcagcag
     6181 ctggcactgc aggcggtgct accgcgcccg cgggtgcaga caccggtgcc ggctggtgcg
     6241 acccgcgtgg cgcagatccc gagcaggcga tcaccgagga caaaacgacc agtctgattc
     6301 gcatcgacgc gcagcatact caggcctggc tcgcctcgcg acgccgcgca cgaactaacg
     6361 tgggtgctta cgggcttgtc accaccatca cccagcgaaa gcgcgcagcg gctccgcgca
     6421 cacgcaagcc gatcctcggc gccgcaggta agaacgctgc gctgccgacg acctcgagcg
     6481 atgggtcaga ggcgtaacat gcataactag tcccggaccg ccgaagtctg agctcgatcg
     6541 ggtcgccagg ggtgagcgca tgcgcagcgc tgttgatttc cgtcgctgtg ctcgcgtcga
     6601 agacgcgcaa tagctcctgg gtcccgtcgg cgaggccggc gagctgacag gcgaccgatt
     6661 tgtccgtgtt gggttccagc cgatcgatca ggttgacgga accgaggttg agcccactcg
     6721 cggtgatcag atcgatgaca acgctggcac gcacctcgac gcgtgcggcc gatggcgacg
     6781 gcatcgtcag cagcgacgtc gcgctatcac cggcaagcag cgacatttcg ccgctgttga
     6841 tcatcgccgt gcccgacgac gtccaggtcg tcggcaacgc accggtgaac ccttcgaacg
     6901 cgacaagcgt gtcgccagcg gtcgctggat tcggatcgca cgcgttgccg actccatcgc
     6961 catcgccatc ggaattgtcg gcaaacggcg ggcacggatc gcatacgtcc ccggtgccat
     7021 cgccatcgtc gtcggcttgg ttcgtgttgg cgacatccgg gcagttgtcg atcgaatcga
     7081 cgacggtgtc ctgatcgcga tccccgtcga cataggcgtc cgcttcccct ccgtcggggt
     7141 acgaacccgg cacctcgcag ctctcgatgc cgtcatgcgc cacacattgt tgttccgccg
     7201 gacagcgcgg ctgagtaccg ggcgccgcgc acggcgcgcc ggtcggagca cgtggcgaga
     7261 aacagctcgc gaacaggacc gcgacagatc cagtacaacg ccgcatcgcg cgcgcatcct
     7321 actgcaagtg atcgcgctgc gaggttggtt atcgtcgttg tgtgacaacg agcgcaaaca
     7381 gttgtttgca gttgcgacga gctaacgcgg attcttcgcg atgatcttct tgacctcctc
     7441 ggcgctgaac gcacgcgaag tcgtcgtgcg cacgttgccg agggagctca cttgaagcga
     7501 cagcgaggcg gctgtctcgt catccggcgc ttcgaacagc gcgacaacgt cgtagtcgcc
     7561 cgtcgtccac acttcctgga tgaggcgacc acctgcttcc ttgatcgccg cccaccactt
     7621 ttctgcacgc gtgacggtgt ccttgtagtt gcgaatccct tggtcggtca gtttcgctaa
     7681 tgtgacgtac atcggcatgc accacgtggg tgcaacgcac acgccacggc ctcaccacag
     7741 cctgtaactg actcttcacg gccatgcgca accccgcgaa gtccccgtga gcacctctgg
     7801 catcaggtct tgcagtagcg tggagccatg atgaggtggg tccttgtcgc gtgcgtcgcg
     7861 actgcttgtg gtggctcgtc gaagcacggc ccgactgtcg atcccgaacg cgcgcgcatc
     7921 gaggcgatgg agccaccatc gccgtacgtg acgcagtcgg tccgcggtta cacgtcgccg
     7981 actgcgtgtt cgcagggacc gttccgcatc gaggcgagcg cactcggcgc gcacttcggc
     8041 gaacagctcg aggtgagtat ctgcgcgccg cgcagtctgc agggcgacta tcagttcacg
     8101 aagggaaccg atcgcgacga ggtcaaacac ttcggcgatc gcaacaacag cgagaagtgt
     8161 ctgccgaccg cagacgagct cgcgcgttac ggcgcggtta gtggcggcgc gagtggcgga
     8221 ggcggcacca gcgtcgcgag cggcggcgga ggtgccacga gccaatcatc gcgcgcgacg
     8281 agcgttgcat cagccacggc ggcgtccgca ccagtccagc tcgccgaggt aagcggcacc
     8341 gtcgccgaga agtgcccgca cggcacctat ctcagcggca tcctctacta cacgatcgtg
     8401 agcagcggcg atcacgaagc ctggaagcag ggcatgtcgc tcgtgctcga cgtgtggtcg
     8461 accgagccgc tcgatctgac cggcgccgtg ttcgtcgtga tccagcgcgg cgttcgcgcc
     8521 gacatgaccg ccgatgcgtg gaagaagtac aacgaggact gggaccgctg gaacaagctg
     8581 tggaacgcgt acctcgatgg cgaggtcacg gccggacgcg ccacgctcgt ggacaacacg
     8641 gcgcgctcca gcgccgcgcc cccacccgcg cgcaccgaag tgaaaccgcc gaagccgagc
     8701 gtgcacgccg aatggatcgc gggcttctgg cagctgcgtg gcgaatggat ctggagtgct
     8761 ggattctggc gcgtgcccga gtccgacatc gtcgcggagc agacggtcga agcacccatc
     8821 gcgccgccac cgccgaaggc cgaggctcca ccaccgccac ccgcaccgcc tgcatcacca
     8881 ccgccggcgc cgaccgtcac ggccagcgcg accgtgacag tcacgccacc cgcgcctgcg
     8941 gcgaccgcga acctcgtgtg gacaccgggc tactgggcgt ggaacggcac cgcgtacatc
     9001 tggatcgaag gcgcgtggcg aatcccgccg caggcgaacg cgcgctgggt tgcgccgacg
     9061 tggcaaccgc gtcgtggccg cgtcgtgatg gtgccaggcg gctggtcggt ttccgtgcac
     9121 atcgggcgct agcaacgagc cacgcgacta ggatgcgcgc atgttgcgcg ctgccctcgt
     9181 cctgtctgtc gtgctcttcg cctcgcccct tcacgctgcg ccgaccctga tcaaagcggc
     9241 gcacgtgctc gacggtgtgt cggcgaaggc gaaagacaat gtcgcgatcc tcgtcgacgg
     9301 cgatcgcatc accgcggtcg gcccagccgc ggagctcgcg aagaaggcgc cgaatgccaa
     9361 ggtcatcgac cttggcaacg cgaccgtatt gcccggactt atcgatgcgc atacacacgt
     9421 gttcctcgac gacgacgtcg ggcctggcgt gtacgacgcg atcctgctca agcagagcgt
     9481 gccgtatcgc gcgatcgcag ccgctgcaaa cgcccgcaag gcgctgcact acgggttcac
     9541 gacgctgcgt gatctcgaga ccgagggcgc gatgtacgcc gacgtcgacg tcaagaccgc
     9601 catcatccgc ggcatcaccg aaggaccgcg catgcaggtc gcaacgcgcg cgttcgcgcc
     9661 gaccggcatg tatccgctca agggctacaa ctgggagctc acgatgccga tcggcgtaca
     9721 gacgatcgac ggcgtcgacg aggcccgcaa ggcagtccgc gagcaggtcg ctcacggcgc
     9781 cgactggatc aagttctacg ccgatcacgg cgtctacgaa ggcaagccgg acaaacccgt
     9841 acgctcgatc gtcaacttca ccgccgcaga agcagccgcg atcgtcgacg aggcgcacag
     9901 gctcggcgtg aaaatctcgg cgcacgcgaa cggctgggac ggcatcgatg ccgcgttgcg
     9961 tgcgggtgtc gactcgatcg aacacggcca ggggctcacc gacgacttgc tcgtgcgcat
    10021 ggtgaaacag cgcgtcgcct ggtgcccgac gctgctcgcg tatcgctatt ccgagcagaa
    10081 gctcggcaag aatgcgcgct cgttcatgat ggcaccgcac gcgcgcgcgc tggccgccgc
    10141 gcacaagcga ggggttcaga tcgtattcgg caccgacgcg ggtgcatttc cgtggaccat
    10201 gaatccggcc gcagaagcga agctcatggt cgatgccggc atgccggccg ccgacgtggt
    10261 tcgcgcgatg acgtcagccg ccgcgacgct gctcgatccg ttctgccgtg cgggcgcgaa
    10321 gcaatgtgcg agctcggatg tcggcgtgat cgcggtcgga aagctcgcgg atcttgtcgc
    10381 cgtcgccggc gatccgctcg aggacatcac gcagctcgag cacgtgaaat tcgtgatgaa
    10441 ggccggcgtc acgtactgac gagcgcagat cctgccgccc gcttgcgcag attccagaac
    10501 cgcggtgcgc gccgccgtgc gaggatccaa acgtgaggtt ggtcgtctgc gggctgtgca
    10561 tcgtcgctgc ggggtgcggt cgcctaggat tcggcgaacg gaccggcggc ggcacaggtg
    10621 acggcggcgg cggctccgac gacggtcagc tcgatgacgg cggtggctcg ggtagcgtcg
    10681 acggcccgat gggcgacggt ccgggcaccg atgcgttcgt gtgcgtgacc ggccccaacc
    10741 acgacgagga cggcgacggc gttcgcgatg cctgcgacgt ctgcccgcac attcccgatt
    10801 cggcgcaggc cgacaccgac ggcgatcgcg tcggcgacgc gtgcgatccg gaaccgaaca
    10861 acgcgcgcca atcgatcctg ctgttcgatc cgttcgtcac gatcggcgcg agctggacca
    10921 atggcggcgc gactcagggc accgacgaga tcatcatcga tgcacgcggc ggcaaggcgt
    10981 tccggatcac ccgatcgatc acgccgacgc acgacctgtt catgatcggc gcgaccacgg
    11041 gtgcgggcga tgcttcgacg caccacatct cgctcgtcac gtttccgaac accggcaacg
    11101 ccgacgcgta ctgcgagatg tacgacaacg gctcgagcac ggccacgcag ttcacgtgga
    11161 cgttcgacaa catgacctac atgcacggag cgccctcgtc gtggggcgcc acgcggctcg
    11221 cgaacggctc cggcacgttc tcgtacgatc tcgacgtcag ccaagtgacc tgcaagtcga
    11281 cgtggcagag caaccagcgc atcagcacgg ccgcacgccc cgcgatcgcg gcggagaagt
    11341 tcatgatcta cggcgagaac ctgctgatgc gcgtgcagta catcatcatc attcgcacga
    11401 actgatggac acgctcacga tcgcgcgtca gctggcggac ttccatccga gcgcacgcct
    11461 gccctgtccg gcgtgtggcg tgagtgtgaa tggcgacaat ctcgcgaagc acctcgccaa
    11521 ggtccacgcc gacttctcgt cgctcacccc accgtggcgc ggacgcggct ggtttggcct
    11581 attgccggca agcatcgcct tcgacggtga cgcgatcgtg ctgcgccgcc tcggacagcg
    11641 gcgcgtgtcg cttccgtgca agctcgaggt gggcaccctc gttggcacgc ggcccgacgc
    11701 gatctcggcg aaccaatccg tggactacaa cacgcccgga gagaccgtgc gcgtcggccg
    11761 ctacctgcgg ctcgtcggcg atcgctcgat cacgatcggc tgccgcagcg cgacgcagtt
    11821 ccatgcccat tggcggcgcg agcaactcat cgatggcggc cggcggcgga gttgtcacgt
    11881 catgctctcg cgcccggcac tcgcggcgat cgaatacgag ctcgcgcgtc gcggcatgct
    11941 cgttccccag tcgcagtgac gtcagcgcgg cgcgagcgtc tgccagtcac tcacacagtc
    12001 gggcgggcac tgatccacgg aggtgccttt gacgtggaag caaggcctcc ccagctcgtc
    12061 ggcaccctcg ctgaccgacg cgcacacgac acgtacgcag tctccgacat cgagaacgcg
    12121 aagccacgca tcagccatgt cgagatccat ctcgatcgat acgcgagccc tacgtatctc
    12181 tgcatccccg agctcgcgaa cgcgccacgg cgatagcgcg agctcgaggg tcatcatgcc
    12241 tgcgccgagc tcgcgcgcgc ccaccacctc cgcgaccagc ccttcgagct cgaatacttt
    12301 ctcagccggc ggcatatgca tcatgcggtc gaaacggtgg tcgcggatgc tcgaggtaca
    12361 cgcgcatcga cggtcgactg acggcgctat cgaggaatgc tttcacgcga tccggaagcg
    12421 gatagctcgt ccggtcgaga cgcagcaatg tcagcgcgag atccgcatgc gccatgttcc
    12481 agtgcgcggg atcgaggtca ccggcggcaa gaaggcgctc ggtgagagca acgagctcac
    12541 gcgcctcgcg ctcgcacgca cgcgacatcg gaggtggctg cggctcggga tacacgcaca
    12601 tccaccacga gcgttcggtg cgcagcgccc acacgtccgc gcggacgaac gccatcagct
    12661 ggcgcgcctt tgctcgcgca taggtatccg ccggcaacag ccgcgggtac gccggaggtg
    12721 ggaacacctc ttcgaggtac tcgatgatcg cgctcgactc ggcaagccag acatcaccgt
    12781 gctgcaacgc cggcacgcgc gacacgttgg tacgactgac cagcgccggc ggcaccccac
    12841 cgccgtcccg cagcagcgcg cgcgccttcg aatagaccag ctgcttttct tcgagcgcgg
    12901 tcacgcacga cgcatcccac tggttcacga agtaaccatc gacatacagc gtgagcgtcc
    12961 gctcgcgcga tgcgttgtgg gaatcggtca ccaagccgac ggtagcatca cggcgcgtcg
    13021 caccggcgcc aactgccagt cgctctcact accccgccgc gcggatcgcc ggcagggact
    13081 ctgtcgcctt ggaaacgcgg atctcggaca tcgccttgcg caggcgctcg ctgccgaacg
    13141 gccgcagcac cgtctcgcgg acgcctagca cctgccgcag gtcggactcg acacggccga
    13201 gcgcgatcag cgcgccggtc caccgatcgc cgcgcacgtt caacagcgcc tggatgtccg
    13261 cttcggtcag cgcatcaaag tcgacgatcg cgatcatggt cgggcgttcc gccactcgag
    13321 cgacgaggtc tgcgacgctc gcagcaacga cgacgcgaat cgcttcgtcc gtcagctcct
    13381 cgttgatcca tcgcaccgtg gacgtacgag gtgcataggc cactactgtc cgtcgtacac
    13441 gctgccgccc cggagccata gttctcgacc atcgtacgaa agatcacgcc ggcagtcggt
    13501 tacgagtggg gatagcgcga agcgcgcaac gcctgcgcac cctaacgtcg cgagttgcct
    13561 gcgacttcga tcgtttcgct gacgagacag tctggtcagc tccacgccgg gcgagatcgc
    13621 tattcgatag cctcgaacgg ctggcccgga atccaacccg gcagcgcggc cgcctgtttg
    13681 atccacgtcg ttagctgcgc ctcgtcgagc gtgtcgtcct cgtgaatgtc gaggtaccgc
    13741 gtgcccttgt tcttgctcgg cccgggcggc agtggtcgca gcgcggtgcc atggaagaac
    13801 gtcagcttca cgtagcgcgc gaagacgtga aagctgagga accagccctg cccttcgagc
    13861 ccgtagagcg gagagttcca cttcaccgct ttgcacacgt tcggcacgct tcgcacgatc
    13921 agcgcgtcga gccgcttgcc gagcgcacgc ttccagtccg gcatcgcagc gatgtacgcc
    13981 tgcacgggcg catcgccgtc ggccatcgcg atctgcggat tgccgcccga gagcagaaca
    14041 accggtttcg ctttcgcctt ggctggcgac ttgcgcggcg cactcgtacg cgacgtcgcc
    14101 ttcttcggct ttgctttgct gcgcatcact tcttgctctt ggctgctttc gctcgagcct
    14161 tcgcaggcgc cttcttcttc accttgctct gattgaacac gatcgcggcg cgcaccatgt
    14221 tctcgagcgc tcgctcatcg accttgtcgc cctcgaacag atcgagcgcg cgccactggc
    14281 cgccatcgag gcccgcgttg aacagcttgt ccggatcagg aaggcgcgcg ccgttcgaga
    14341 acgtcagctt caccttgtcc ttgtgcgcgt tgcccaccgc gatcatgcca tcgcgcgacc
    14401 acaccgggct tcccatccac ttccattcct cgatgatctc tcggtccacg tcgaggatga
    14461 tcttgcggac cttcgcgagc gtcttgccac gccaatcgct gagtcctgca atcagctggt
    14521 cgatgcgttc cgagggattc atgggatgtc tctagcgcga cagctgggtg atttgaatga
    14581 tgttgccgca cgtgtccttc aacatcgcga tcgtcgcgaa cgtcacgtcg gtgggcggca
    14641 tcgtgaactc ggcacccttc gccttgatgc gctcgtattc gcccttcacg tcgtccgtgt
    14701 agaacatcgc cgcgggctgg ctctgcttga acatcgcctc ctggtacgcc ttggccgctg
    14761 gatcactgtt gagcgccagc agcagcgccg tgccctctcc gtcgccgggc gccgtcaccg
    14821 tgagccagcg aaacgagccg ttcgtctcgt catccttctt cgcgaagccg agcacgtcgg
    14881 catagaagcg caggcacttc tcctgatcgt cgacgtagac gtgcgcgagc ttgatgttga
    14941 gcc
//
LOCUS       DASUFX010000581         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_373151,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000581 DASUFX010000000
VERSION     DASUFX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373151"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFV99_23445"
     CDS             <1..496
                     /locus_tag="VFV99_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062354.1"
                     /translation="AVGIALVVLDGALPARVGAAFELAVAAMLIALGARAVIRGVRNT
                     DGEPVPHRHFGVEHVHGGAGSHVHVGARAVAWRPLTVGIVHGLAGSGALTALAFAELP
                     TSSARVLYMMVFGAGSVAGMAIATGLAGIALQRVARGAATRRWLAITTGLISCGVGVS
                     WALG"
     gene            632..3067
                     /locus_tag="VFV99_23450"
     CDS             632..3067
                     /locus_tag="VFV99_23450"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5062355.1"
                     /translation="MANRGDVLAKGFIRELDGLRGIAILMVMVHRFWPRTGVGVAADV
                     AGAGWIGVDLFFVISGFLIAGILLDTRGDTGYFRNFYARRALRILPLYYLFVIGVFVA
                     FAHNAEFLARAGSPFWYLVHLGNVPEGLLGLNTPYWIAPVWSLAIEEQFYLTFPWLVN
                     GVDRRRLTIVLVSMIVIAPVIRIITMFEFPDQERVQYLFTLCRIDTIAIGCLLAVVMR
                     SVDVERWRHRATLGALAVLPTVVVIAIASRLDRTSPFDRVFGYSVVAVGCVCVVGLVV
                     MSRGSRATAPLRFKPLTYLGKLCFGLYLLHRPADTIVTAIGARLGIRHDLWLLLPKIA
                     VATALATISWRILEQPFLRLKDRFATARHPSAEVAPPPTVALPTRPGFVTRALRMIGI
                     LLVLLVSSCGDDSARNPDGGVVGDADAGLDPDIGSGSDIDASVVPVRVLYPEGMRHSP
                     ITPAIVAGLQSIASSGQRANVFAKVGDSITVAPEFLFCFDGGTVDLGGRTDLSPTLAY
                     FRAGNAAGTSPFARDSYAARSGTTAQDALAGNPSALEREITTIAPQLAVVMFGTNEAR
                     FSYSLEQFAGPLWDVIDGCIARGVVPIMSTIPPSNASSSADARVPLYNRVIRAIAQGR
                     GVPLVDFHRELVSLPSRGISSDGIHPSVSPSGGCVLTAAGLRYGYNVRNLITLEALAR
                     THAAIAGTPADSDAPVRGGTGSEADPFTGPLPFVDIGDTTAGVAHVGHSCGSASGHEV
                     AYRVMVPSARSLSVYVVDRATTNVDIRILVNGACVGSGDASASASVPAGNVDVLVDAR
                     TPNSEGEYVVVIQ"
     gene            3206..4579
                     /locus_tag="VFV99_23455"
     CDS             3206..4579
                     /locus_tag="VFV99_23455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5062356.1"
                     /translation="MTRAPRITFLGATDTVTGSRYLVEHDGVRILVECGLFQGPKKLR
                     VRNWEPPTFDVTKLDVVILTHAHIDHSGYVPRLCKLGYRGRIYCTDGTRDLLHILLPD
                     AGYLQEEEARLANREGYSRHKPALPLYTREDAVASLDQLFAVGFGESFAPAAGVTATF
                     TRAGHIVGSSCLRLALGGTSVTFTGDVGRPDDLVMKPPDRLPQTDYLVVESTYGDRRH
                     PACDVAEDITRVVNETYERHGTLVVPAFAVGRAQHLLYVLAELKAKRRIPDIKVYLDS
                     PMAIDATKLFCDHKLEHRISESACTLMCKLPEYTHTADQSKAIDRSNTPSIIISASGM
                     ATGGRVLHHLRRFLPDARSTVLLVGYQAAGTRGRSLLDGADELKIHGQYVPVKAKIVQ
                     IEALSAHADYREMITWLRASNIHPKRVFVTHGEPQAADGMRRRLVEEFGWTVSAPSFG
                     DQGVLEP"
     gene            4576..5211
                     /locus_tag="VFV99_23460"
     CDS             4576..5211
                     /locus_tag="VFV99_23460"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062357.1"
                     /translation="MTRCACTEIHPARRIVLTGGPGAGKTAVLEMMRKTLCEHVRIVP
                     EAASIVFGGGFPRGNALEQRRAGQRAIFYVQRELEAAIMEENAAIALCDRGTIDGLAY
                     WPGPDELWPSVGTTLDEQLARYHAVIHLRTPPVESYNRQNPLRIESAVEAAAIDTRIA
                     GAWAKHPRRFEVDSMPDFIAKVARVVAIVRGELPECCRKHPVAELGERRGA"
     gene            5334..>6428
                     /locus_tag="VFV99_23465"
     CDS             5334..>6428
                     /locus_tag="VFV99_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX5062358.1"
                     /translation="MSQHHPHSHGHANAHHQHDAMPGSDAARDPVCGMTVKPTTAHRT
                     MHDGHEVLFCSARCREKFLANPAQYTKSAEHTHAKAPPAPAVPAPTHPVAGTVEYTCP
                     MHPEIVRDAPGSCPICGMALEPRVPTLEAGPDPELLAMTRRFWISAGLSLPLLVIVMS
                     ERVGVTLLAGRTRVWVELALALPVCTWAAWPFYARFAQSLKNKSPNMFTLIGLGVGVA
                     FIYSLVAALAPGIFPDAFRNHDGTVGTYFEAAAVITTLILLGQVLELRARSSTGAAIR
                     KLLGMAAKSARRIKEDGSEEDIPLEHVHVGDKLRVRPGEKVPVDGVVLEGVSSVDESM
                     VTGEPIPVEKHQGERLIGATVNGTGALVMRA"
BASE COUNT         1029 a         2214 c         2064 g         1121 t
ORIGIN      
        1 cgcggtcggc atcgcgcttg tcgtgctcga cggcgccctg cccgctcgcg tcggcgctgc
       61 gttcgagctc gcggttgccg cgatgctgat cgcgctcggc gcgcgtgcgg tgatccgcgg
      121 cgtgcgcaac accgacggcg aaccggtgcc gcatcgtcac ttcggcgtcg agcacgtgca
      181 tggcggcgct ggctcgcacg ttcacgtcgg tgcgcgcgct gtcgcgtggc ggccactcac
      241 cgtcggcatc gtgcacggtc tcgccggcag tggcgcactg accgcgctcg cgttcgcaga
      301 gctgccgacc agcagcgcac gtgtgctcta catgatggtg ttcggcgcgg gctcggtcgc
      361 cggcatggcg atcgcgaccg gactcgccgg catcgcgctc caacgcgtcg cgcgtggtgc
      421 ggccacgcgg cgctggctcg cgatcacgac gggcctcatc tcgtgcggcg tgggcgtgag
      481 ctgggcgctc ggctagtgcg tagcttcgcg taatcgccag cgcgacatgc gcgcgtcggt
      541 cgtgcggctg tgcgcctgcg cgctagcgcc ggcgctcaac ccatgatctc gcacgaacaa
      601 tcccggcata tcggctgcac tactgacccg catggcaaat cgcggcgatg tgctcgcgaa
      661 gggcttcatt cgagagctcg atggcctgcg cggcatcgcg attctcatgg tcatggtgca
      721 tcgcttctgg ccgcgcaccg gcgtgggtgt tgcggcggac gtcgcgggcg caggttggat
      781 cggcgtcgat ctgttcttcg tgatcagcgg gttcttgatc gccggcatct tgctcgacac
      841 tcggggcgac accggctact tccgcaactt ctacgcgcgt cgcgcgttgc gcatcctgcc
      901 gctctactac ctgttcgtca tcggcgtgtt cgtcgcattc gcgcacaacg ccgagttcct
      961 cgcgcgtgcc ggatcgccgt tctggtatct cgtgcatctc ggcaacgtgc ccgaaggcct
     1021 cctcggtctg aacacgccgt actggatcgc gccggtgtgg tcgctcgcga tcgaggagca
     1081 gttctatctg acgttcccgt ggctcgtgaa cggagtcgat cgccgcaggc tgacgatcgt
     1141 gctcgtgagc atgatcgtga tcgcacccgt gatccggatc atcacgatgt tcgagttccc
     1201 ggaccaggaa cgcgtgcagt acctgttcac gctctgccgc atcgacacga tcgcgatcgg
     1261 ctgcttgctc gccgtcgtca tgcgatcggt cgatgtcgag cgctggcgcc atcgcgcgac
     1321 gctcggcgca ctcgccgtgc tgccgaccgt cgtcgtgatc gcgatcgcga gccgtctcga
     1381 tcgcacgagc ccgttcgatc gcgtgttcgg ctacagcgtc gtcgcagtcg gctgcgtgtg
     1441 cgtcgtcggg ctcgtcgtga tgtcgcgcgg cagccgtgca actgcgccgc tccggttcaa
     1501 gccgctcacc tacctcggca agctgtgctt cggcctctac ctcttgcatc gtccggccga
     1561 cacgatcgtc accgcgatcg gcgcgcggct cgggatccgc cacgatctct ggctgctgct
     1621 gccgaagatc gcagtcgcaa cagcgctggc gacgatctcg tggcgcatcc tcgagcagcc
     1681 gttcctgcgc ctgaaggacc gcttcgcaac cgcgcgccat ccgagcgccg aggtcgcgcc
     1741 gccgccgacg gtcgcgctgc ctacacggcc cggcttcgtc acgcgcgcgc tgcgcatgat
     1801 cggcatcttg ctcgtgctgc tcgtctcgag ttgcggtgac gacagcgcgc gcaatcccga
     1861 cggcggcgta gtaggcgacg cagatgccgg cctcgatccc gacatcggat caggttccga
     1921 tatcgacgcg agcgtcgtgc ccgtgcgcgt gctgtacccg gaaggcatgc ggcactcgcc
     1981 gatcaccccg gccatcgtcg ccggcttgca atcgatcgcc agctccggtc agcgtgcgaa
     2041 cgtgttcgcg aaggtcggcg actcgatcac ggtcgcgccg gaattcctgt tctgcttcga
     2101 cggcggcacc gtcgacctcg gcggccgcac cgacctgtcg ccgacgctcg cgtactttcg
     2161 tgccggcaac gcggccggta cgtcaccgtt cgcacgtgac agctatgccg cacgcagcgg
     2221 cacgaccgcg caggacgcgc tcgccggcaa tccatcggcg ctcgaacgcg agatcacgac
     2281 gatcgcaccg cagctcgccg tcgtcatgtt cggcacgaac gaagcccgct tcagctactc
     2341 actggaacaa ttcgccgggc cgctgtggga tgtgatcgat ggctgcatcg cgcgcggtgt
     2401 cgtgccgatc atgtcgacga ttccaccgag caatgcatcg tcgtcagccg acgcgcgcgt
     2461 cccgctgtac aaccgcgtca tccgtgcgat cgcgcagggc cgcggtgtac cgctcgtcga
     2521 tttccatcgc gagctcgtct cgctaccgag tcgcggcatc tcgagcgatg gtattcaccc
     2581 gagcgtatcg ccgagcggcg gctgcgtgct caccgcggca ggcctgcgct acggctacaa
     2641 cgttcgtaac ctcatcacgc tcgaagcact cgcgcgcacg cacgccgcga tcgccggaac
     2701 cccagccgac tccgatgctc cagtgcgcgg cggcaccggc agcgaagccg atccgtttac
     2761 cggcccgctg ccgtttgtcg acatcggcga tacgactgcc ggcgtcgccc acgtcggtca
     2821 cagttgcggc agtgcgagcg gccacgaggt cgcctatcgc gtcatggtcc cgagcgcgcg
     2881 ctctctctcg gtctacgtcg tcgaccgcgc cacgacgaat gtcgatatcc gcatcctggt
     2941 caacggtgcg tgtgtcgggt cgggcgacgc gagcgcgagc gcgagcgtac ccgccggcaa
     3001 cgtcgacgtg ctcgtcgatg cccgcacccc gaacagcgag ggcgagtacg ttgtcgtgat
     3061 ccagtagctc ccggttcgcg cagctcgtgc agcgtgcgcc acaggtacgc aatggttgcg
     3121 cgagtcctcg cgtcttcaac cgactcggcg cggcacgtcc cttgaaatct acgatcacgg
     3181 caagtttgcg aacggcggtt ccgtgatgac ccgcgcaccc cgcatcacct tcctcggcgc
     3241 gacggacacc gtcacgggat cacgatatct cgtcgagcac gacggcgtcc gcatcctcgt
     3301 cgagtgcgga ttgttccaag ggccgaagaa gttacgcgtg cgcaactggg aaccgcccac
     3361 cttcgacgtg acgaaactcg acgtggtcat cctcacgcac gcgcacatcg atcacagcgg
     3421 ctatgtgccg aggctatgca agctcggcta tcgcggtcgc atttactgca ccgacggcac
     3481 acgcgacctg ctgcatatcc tgcttcccga tgctggttat ctgcaggagg aagaagcacg
     3541 ccttgccaat cgcgaaggct actcgcggca caagccagcg ctgccgctct acacgcgcga
     3601 ggatgccgta gcctcgctcg accagctctt tgccgtcggt ttcggcgaga gcttcgcacc
     3661 ggcggccggc gtgaccgcca cgttcacgcg tgccggccac atcgttggct cgagctgtct
     3721 gcgccttgcg ctcggcggaa catcggtgac gttcactggc gacgtcggac gccccgacga
     3781 tctcgtgatg aagccgcccg atcgtttgcc ccagaccgac tacctcgtag tcgagtcgac
     3841 ctacggcgat cgtcggcacc ctgcctgtga cgtcgccgaa gacatcacgc gcgtcgtcaa
     3901 cgagacctac gagcgacacg gcacgctcgt ggtccccgcg ttcgcggttg gccgcgcgca
     3961 gcacctcctc tacgtgctcg ccgagctcaa ggccaaacgg cgcattcctg acatcaaggt
     4021 ctatctcgac agcccgatgg cgatcgacgc gacgaagctg ttctgtgatc acaagctcga
     4081 acatcgaatc agcgagagcg cgtgcacgct gatgtgcaag ctgcccgagt acacgcacac
     4141 tgcggatcag tcgaaagcga tcgatcgcag caacacgccg tcgatcatca tctcggcgag
     4201 cgggatggcg acgggaggcc gcgtgctgca ccatctgcgg cgtttcctgc ccgacgcgcg
     4261 cagcacggtg ctgctcgtcg gctatcaagc ggcgggaacc cgcgggcgct cgctcctcga
     4321 cggcgccgac gagctcaaga tccatggcca gtacgtgccg gtgaaggcga agatcgtcca
     4381 gatcgaggcg ctgtccgcgc atgcggacta tcgcgagatg atcacgtggc tgcgcgcgag
     4441 caacatccac ccgaagcgcg tgttcgtcac gcacggcgag ccgcaagcag ccgacggaat
     4501 gcggcgccgc ctcgtcgagg aattcggttg gacagtcagc gctccgagct ttggtgacca
     4561 aggagtgctc gaaccatgac ccgttgtgcc tgtaccgaga ttcatccggc gcgccgcatc
     4621 gtgctcaccg gtgggccggg cgccggcaag accgccgtgt tggagatgat gcgcaagacg
     4681 ctgtgcgagc acgtcagaat cgtgcccgaa gcggcgagca tcgtgttcgg cggcgggttc
     4741 ccgcgcggga acgcgctcga acagcgtcgg gccgggcagc gtgcgatctt ctacgtccag
     4801 cgcgagctcg aggcggcgat catggaggag aacgcggcga tcgcgctgtg cgaccgcggc
     4861 accatcgatg gcctcgccta ctggcccggt cccgacgagc tgtggccgtc agtcgggacg
     4921 acgctcgacg agcagcttgc ccgctatcac gccgtgattc acctgcgcac accgccggtc
     4981 gagagctaca accgtcagaa cccgttgcgc atcgagtccg cggtcgaagc agcggcaatc
     5041 gacacgcgca tcgcaggtgc gtgggcgaaa catccacgtc gcttcgaggt cgactcgatg
     5101 ccggacttca tcgcaaaggt cgcgcgcgtg gtcgcgatcg tccgtggcga gctgcccgag
     5161 tgctgccgca agcatccagt cgccgagctc ggtgaaagaa gaggtgcgtg atggaaatcc
     5221 gaagctacgc gagcatgccc gcaagccgct aaccaaccag tctgattgga gtcatccttg
     5281 taggtgtaat gaagtcgaac cggccgcgtc gtagggccga ggacgcaagc aacatgtcgc
     5341 agcaccatcc gcactcgcac ggccacgcca acgcgcatca ccagcacgac gcgatgcccg
     5401 gtagcgacgc tgcgagggat cccgtctgcg gcatgacggt gaagccaacg accgcgcatc
     5461 gcacgatgca cgatgggcac gaggttctct tctgcagcgc gcgctgccgg gagaagttcc
     5521 tcgcgaaccc cgcgcagtac acgaaatccg cggagcacac gcacgccaaa gcgccgcccg
     5581 ctcccgccgt ccccgcgccg actcaccccg tagcgggtac ggtcgagtac acgtgcccga
     5641 tgcatccgga gatcgtccgc gatgccccag gcagctgccc gatctgcggc atggcactcg
     5701 agccgcgcgt gcccacgctc gaggctggtc cggatcccga gctcctcgcc atgacgagac
     5761 gcttctggat ttcggccggg ctgtcgttgc cgctgctcgt gatcgtgatg agcgagaggg
     5821 tcggcgtcac gctgctcgct ggccgcacgc gcgtctgggt agagcttgcg ctcgcgctgc
     5881 ccgtttgcac gtgggcagcc tggccgttct acgcgcgttt cgcgcagtcg ctgaagaaca
     5941 agagcccgaa catgttcacg ctcatcgggc ttggcgtggg cgtcgcgttc atctacagcc
     6001 tcgtcgcagc gcttgcacca ggcatctttc ccgacgcgtt ccgtaaccac gacggcaccg
     6061 tcggcacgta cttcgaggcc gctgccgtca tcacgacatt gatcctgctc ggtcaggtac
     6121 tcgagcttcg tgcgcgaagc tcgacgggcg ccgccattcg caagcttctc ggcatggcag
     6181 ccaagtcggc gcgccggatc aaggaggatg gcagcgagga ggacattcca ctcgagcacg
     6241 tccacgtcgg tgacaagctg cgcgtgcgac ccggagagaa ggtgccggtc gacggcgtcg
     6301 tgctcgaagg cgtgtcttcg gtcgacgaat cgatggtcac cggcgaaccg attccggtcg
     6361 aaaagcacca aggcgagcga ctgatcggcg ccaccgtcaa cggcaccggc gccctcgtca
     6421 tgcgcgcc
//
LOCUS       DASUFX010000582         8301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1762484, whole genome shotgun sequence.
ACCESSION   DASUFX010000582 DASUFX010000000
VERSION     DASUFX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762484"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFV99_23470"
     CDS             <1..209
                     /locus_tag="VFV99_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062359.1"
                     /translation="VVDTSLGFKEAKERWNDQFERRYVSLVFAANQNNVTRAAEHAGL
                     SRRHFRELLYKHGIVERPADDDNE"
     gene            206..1867
                     /locus_tag="VFV99_23475"
     CDS             206..1867
                     /locus_tag="VFV99_23475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062360.1"
                     /translation="MKRFGAYEILHELKSGGMGSVLLARRKGPGAFEQLVAIKTIRPE
                     HAGTPAVRAMFLDEAAILARLNHPGIATVHDFGEEAGTLYMVMEYVAGIPFRELVEHR
                     PPPTVLARAIAEACRGLHAAHELRDLTGHPLDLVHRDISPDNLMLGFDGHVKVIDFGI
                     ALVKKNRQAPVTEFGTVKGKPPYMSPEQVANEPMDRRSDIFSLAVVLWELLTQRSLFE
                     GDSIYAIAFAVGNQTILPPSQYAGALPYGLDAAIMNALDRDLARRTPTAAAFAEQLEQ
                     VIQHAGGETLEAWAERVLATPRAEHRTWLADVISGTELPKIVGRATGQVTELAPIGAK
                     TPQAAIPAAMIPAAAPLVPEHKTPTSIGGTAVTDGLSRPPRSRVAPVVVLLLLLLAIG
                     GGVFMLMRKQTPTVVTREDAWPPPPVTADATVVVPVPADAPADAELADAPTDARIDGG
                     TRVVTIRPDAGRSSPRDAAVALVRPDAAEKATGTGYFTAKYKPGVFANITIDDGTPFP
                     GPVLKPRAIPTGRHTIKFIHPKGDLLDTQVITVEDGKTVDIRQRD"
     gene            2296..3144
                     /locus_tag="VFV99_23480"
     CDS             2296..3144
                     /locus_tag="VFV99_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062361.1"
                     /translation="MFGDDAAYEAAAIETTTAVTDAMASDPTVTALESAPAGERHRVL
                     IAWGRMPADPNATTGRNWSGSLTVSRGAIVVGRTLGFEDATDQLLPRATRDTVSFQSV
                     TRPFADGVLLRVLDPDPTQGAMHLTYTSADAATTYDLDLSQLVNGPISIDAGDGNRLI
                     AVALRELDGCDHGFLRGRWVALRPGMGVYRGVVATADGELAGHIRGIWGQRKNGDKVM
                     FGKFIDTDGHFRGILAGTYEAGQWHARWIVRSGDHGVAGGLYFDAPNVRGGGFLGRWA
                     ETSCAQ"
     gene            complement(3383..4996)
                     /locus_tag="VFV99_23485"
     CDS             complement(3383..4996)
                     /locus_tag="VFV99_23485"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX5062362.1"
                     /translation="MRWLPLVLPVVLHVACARPHVESPRLAVGAGVEKPIASGETHHY
                     AIELAQDQVLFAAIDQLGADVVVSAYAPDGTKLAEVDSPTGAQGSEQIRVDAKQAGTY
                     RVDVKAFPEQHGAYRARVVEIISAREMAEREAKERAIVEKYFTDRSEVVDGVVAWAKQ
                     AAIGDDFAGIEKLVGDARVISLGEADHGVHEYLAYRNRLAKYLIEKLGVTAILVESGF
                     TETTAVDDYVLGIGTASARDVARAVFAWAMPMTLADNLELVEWLRAYNQKAARKVHVY
                     GIDVTGGRNGEMTEGRLAVEASLAYLAQHDRATHAKLEPRLTPLLPRFTVKGYLELTD
                     GQRAELTTAIADLLAAFKTAQDTPELRRARQHAAMAAVLETYFRENRERSLKKESIDD
                     WAASVRDATMAANVIWSLEQEGAGSRVFLFTHNGHARRCERMVDGSTRPAPAAMGQYL
                     AKSLRDKLVVIGFLHGDGPPTMTLDGLFARVGLPSFAVDLRAAPETVRAGLNQPWSLR
                     SNPAWSAKPAQCFDALTFTSTVSSAPLVK"
     gene            complement(4996..5859)
                     /locus_tag="VFV99_23490"
     CDS             complement(4996..5859)
                     /locus_tag="VFV99_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5062363.1"
                     /translation="MRTLTSVLGNAQRLDGGAMFGNAPKAMWEKWIPPDDRNRIPLAC
                     RCLIVRDRGRVILLEAGIGAFFEPALRERYGVVEDRHVLLESLAAVGIRPEDVDIVVL
                     SHLHFDHAGGLLDAWQAGKQPSLVFPNASYVVGAEAWQRAMQPHARDRASFIPGLTDL
                     LVDSGRLELAAAETSQLLGDGFCFHRSSGHTPGLLLTEVAMPAGPVVFAADLIPGKAW
                     VHLPITMGYDRYPEMLIDEKATLLGDLLHRKGRLFFTHDPNIAMGTIAKDDKGRFHVV
                     DERVTLRDVAS"
     gene            5960..7672
                     /locus_tag="VFV99_23495"
     CDS             5960..7672
                     /locus_tag="VFV99_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062364.1"
                     /translation="MRGLLVAFVAALAFTGCGPSTGGDHGDDANATLTIDPPTSELLI
                     DNGILPTEDFTATLTYPDGFTRDVTNEVHFNVDSGYAMFTANTLTAQAAGKTQVFASW
                     TDKLAVAQLIIKLRNVRVDPGLPVGAADWFTNNPEDPTRAPTIVYPPADVVIPRNLGD
                     FEVHWTDATNNVFELSLKTEFTDVRVIVPGGNGSIANSSWQAFLAVEWMAAVSNETTV
                     TYQVRGVDQNNPTVIGSGAPQIVKLSNEQMLGGLYYWAATAQNGPYGIFRHDMAKPGQ
                     PAEQFYTTAQTGGRCVACHVLSRDGTKMAVTYDGGDGMATLVDVATKTPQTTQRGWNF
                     GAFTPDGTKFLANRQGTLTVIDYTTQATLATMTASGYVTHPDLSADGTRLVYVQGPGG
                     TSDWSFGGGKIFTRTFDPATNSFGPEQLLVADTNNNYYPSISPDGQWVLFNRAAGGSS
                     YNNGNATLFVTKADGTGIPIELAKSNQGLGLTNSWGRWAPFQQTIGAANEPMYWVTVS
                     STRNFGVRLVGVNRPQIWMTPFFPTKAAGATDPSNAAFRLPFQNIDSNNHIAQWTEQV
                     VEVQ"
     gene            complement(7735..>8301)
                     /locus_tag="VFV99_23500"
     CDS             complement(7735..>8301)
                     /locus_tag="VFV99_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062365.1"
                     /translation="TAFDMLLALDPAHFLSYKLSPKATLVFEKTLEQAKQRGAPAIDV
                     NWSRGQKVGEPIPIDIEVLADPKQFLSRATLFVRTRGEPTWRAADTQLAKDKRVVLPA
                     VQASKNVQLELYLRAYDQRGNEVLTWADATKPREMVLRYDPPTPWYRKWWGITSIVAG
                     SAAIVGGVVYTVTLSPPDKIDGSVTTAP"
BASE COUNT         1426 a         2833 c         2679 g         1363 t
ORIGIN      
        1 aggtcgtcga tacgtcgctc ggattcaagg aagcgaagga gcgctggaac gatcagttcg
       61 agcgtcgcta cgtgtcgctc gtgttcgcgg cgaatcaaaa caacgtcacg cgcgccgccg
      121 agcacgccgg gctctcgcgt cgacactttc gcgagcttct gtacaagcac ggtatcgtcg
      181 agcgaccggc agacgacgac aacgaatgaa acgtttcggc gcctacgaga tcctgcacga
      241 gctcaagagt ggtggcatgg gctcggtgtt gctcgcgcgc cgtaaaggcc ccggcgcgtt
      301 cgagcagctc gtcgcgatca agacgatccg gcccgagcac gccggcacgc ccgccgtgcg
      361 cgcgatgttt ctcgacgagg ccgcgatcct cgcgcgcctc aaccacccgg gcatcgcgac
      421 cgtgcacgac ttcggcgagg aagccggcac gctctacatg gtcatggagt acgtcgcggg
      481 cattccgttc cgcgagctcg tcgagcatcg gccgccgccg accgtgctcg cgcgcgcgat
      541 cgccgaggcg tgccgtggcc tccacgcggc gcacgagctg cgcgacctca ccggccatcc
      601 actcgatctc gtccatcgcg acatcagccc cgacaacttg atgctcggct tcgacggtca
      661 cgtcaaagtg atcgacttcg gtatcgcgct cgtgaagaag aaccggcagg caccggtcac
      721 cgagttcggc acggtcaagg gcaagccgcc gtacatgtcg ccggagcagg tcgcaaacga
      781 accgatggat cggcgcagcg acatcttctc gctcgcggtc gtgctgtggg agctgctcac
      841 gcagcgcagt ctgttcgagg gcgactcgat ctacgcgatc gcgtttgcag tcggcaatca
      901 gacgatcctg ccgccgtcgc agtacgcggg cgcgttgcca tacggcctcg acgccgcgat
      961 catgaacgcg ctcgaccgcg atctcgcgcg ccgcacgccg acagctgcgg cgttcgccga
     1021 gcagctcgag caagtgatcc agcacgccgg cggcgagacg ctcgaagcgt gggcagagcg
     1081 tgtgctcgcg acgccgcgcg ccgaacatcg cacctggctc gccgacgtga tcagcggcac
     1141 cgagctgccg aagatcgtcg gccgcgcgac ggggcaggtg acagagctcg cgccgatcgg
     1201 tgcgaagacg ccgcaagcgg cgattcccgc cgcgatgatt cctgcggccg cgccgctcgt
     1261 gcccgagcac aagacgccga catcgatcgg cggcaccgca gtcaccgacg gactctcgcg
     1321 tccgccgcgc agtcgcgtcg cgccagtcgt cgtgttgctg ctcctgctgc tcgcgatcgg
     1381 tggtggcgtc ttcatgctca tgcgcaagca gacgccgacg gtcgtcactc gcgaagacgc
     1441 gtggccgccg ccgcccgtga ccgcggatgc aacggtcgtc gtgccggtac ccgcggatgc
     1501 gcctgcggac gccgagctcg cggacgcgcc gaccgatgcg cggatcgatg gcggtacgcg
     1561 cgtggtcacg attcgtcccg atgccggtcg ctcctcgccg cgggatgcag ctgtcgcgct
     1621 cgtgcgtccc gatgctgcgg agaaggcgac cggcacgggc tacttcacgg cgaagtacaa
     1681 gcccggcgtg ttcgcgaaca tcacgatcga cgacggaacg ccgttccccg ggccggtgtt
     1741 gaagccgcgc gcgattccga ccggccgcca cacgatcaag ttcatccatc cgaagggcga
     1801 cctgctcgac acacaggtga tcaccgtcga ggacggcaag accgtcgata tccgccagcg
     1861 cgactgaaag gggtctgacc ggtctgaccc catttccggg cgttgggact gaaaaggggt
     1921 ctgaccgtct gaccccattt ccgggcaaca aaacgtaccc agaagcgcgg gctttccgcg
     1981 atgccggtac ctcgagatac cggttcgcgc ccaaccgctg aacccagtgg taccgcgaga
     2041 gaccggtgct cccggttcgg cgaggcgcga cgccgagtta gccgctggcg cggcggttgc
     2101 tgaatgactt ctccgccgac cacaaggagt cctcttccaa tgcgccacca gcttccactt
     2161 gttcttctcc tcgccagctc tacgtttgcc accttcggat gcgtcgacaa cagcgcctcc
     2221 gccctcgacg ccgaccaagt cacctccgct ctcgaacaag agaacggcgg cttcgacacc
     2281 aacgacgaag cgccaatgtt cggcgacgac gccgcgtacg aagcggccgc catcgagacc
     2341 accacggccg tgaccgacgc gatggcgtcc gacccgaccg tgaccgcgct cgagagcgcg
     2401 cccgccggtg agcgccatcg cgtgctgatc gcatggggcc gcatgccggc cgatccgaac
     2461 gcgaccaccg gccgcaactg gagcggctcg ctcacggtct cgcgtggcgc gatcgtcgtc
     2521 ggccgcacgc tcggcttcga agacgcgacc gatcagctgc tgccgcgcgc gacgcgcgac
     2581 acggtttcgt tccagtcggt gacgcgtccg ttcgcggatg gcgtgttgct ccgcgtgctc
     2641 gatccagatc cgacgcaagg tgcgatgcac ctgacgtaca cgtcggccga tgcggcaacg
     2701 acctacgatc tcgatctatc gcagctcgtg aacggcccga tctcgatcga tgccggcgac
     2761 ggtaaccgcc tcatcgcggt ggcactgcgc gagctcgacg gctgcgatca cggcttcctt
     2821 cgcggccgct gggtcgcgct gcgcccgggc atgggcgtct atcgcggcgt cgtcgcgacc
     2881 gccgatggcg agctcgccgg tcacatccgc ggcatctggg gtcagcgcaa gaacggcgac
     2941 aaggtgatgt tcggcaagtt catcgatacc gatggtcact tccgcggcat cctcgcgggc
     3001 acgtacgaag cgggtcagtg gcacgcacgc tggatcgttc gctcgggtga ccacggcgtc
     3061 gcgggcggcc tctacttcga cgcgccgaac gtgcgcggcg gcggcttcct cggtcgctgg
     3121 gccgagacca gctgcgcgca gtagcaatag cgttgacctt gtcggcgacg tgaacgtggg
     3181 cgccgacatc aagctttgac gttgtcgtcg ctacgcatgc gtggccgacg atgcgccccg
     3241 cggagaacgt acctccggcg gggcgcagct gtttctgcga tgccgcggcg tgtgttcgtc
     3301 cgcgctcgtt cgcccgcgtg ccggccgttc acgtcgccca cacgggtgac gtcgacgtcc
     3361 gcgttcacgt cgaacgtagt cgttacttca cgagcggtgc actgctcacg gtgctcgtga
     3421 acgtcagcgc gtcgaagcac tgcgccggtt ttgcagacca cgccgggttg gatcgaaggc
     3481 tccagggctg attcaacccg gcgcgaaccg tttcgggtgc cgcgcgtagg tcgacggcga
     3541 agctcgggag gccgacacgg gcgaacaacc cgtcgagcgt catggttggc gggccatcac
     3601 cgtgtagaaa gccgatcacg acgagcttgt cgcgcaggct ctttgcgagg tactggccca
     3661 tcgccgcggg agccggtcgc gtcgagccat cgaccatgcg ctcgcagcgg cgcgcgtggc
     3721 cgttgtgcgt gaacaggaac acgcgactgc ccgcgccttc ctgctcgagc gaccagatca
     3781 cgttcgctgc catcgtcgcg tcgcggacgc tcgcggccca gtcgtcgatc gattccttct
     3841 tcaggctacg ttctcggttc tcgcgaaagt acgtctcgag cacggctgcc atcgcggcat
     3901 gctggcgcgc acgacgcagc tccggtgtgt cctgcgccgt cttgaacgct gcgagcagat
     3961 ccgcgatcgc ggtcgtcagc tccgcgcgct ggccatcggt gagctcgagg taccccttca
     4021 ccgtgaatcg cggcagcagt ggagtgagcc gcggctcgag cttggcgtgc gtggcacgat
     4081 cgtgctgcgc gaggtaggcg agcgaggcct cgacggcgag gcggccctcc gtcatctcgc
     4141 cgttgcggcc gccggtgaca tcgatcccat agacgtgcac cttgcgcgcg gctttctggt
     4201 tgtaggcgcg cagccactcg acgagctcga ggttgtcggc gagcgtcatc ggcatcgccc
     4261 acgcgaacac ggcgcgcgcg acgtcccgag cgctcgcagt cccgataccc aggacgtaat
     4321 cgtcgaccgc ggtggtctcg gtgaagccgc tctcgacgag gatcgcggtg acgccgagct
     4381 tctcgatcaa gtacttcgcg agccggttcc gatacgcgag gtactcgtga acgccgtgat
     4441 ccgcctcgcc gagcgagatc acgcgcgcat cgccgacgag cttctcgatg cccgcgaagt
     4501 catcgccgat cgcagcttgc ttcgcccacg cgacaacccc gtcgacgacc tcgctgcgat
     4561 ccgtgaagta cttctcgacg atcgcgcgct ccttcgcttc gcgctccgcc atctctcgtg
     4621 cactgatgat ctcgaccacg cgtgcgcgat acgcaccgtg ctgctcgggg aacgccttga
     4681 cgtccacgcg atacgtgccg gcttgcttcg cgtcgacgcg gatttgttcg ctgccctgcg
     4741 cgccggtcgg cgagtcgacc tccgcgagct tggtcccgtc gggtgcatat gccgacacga
     4801 cgacatccgc accgagctga tcgatcgcgg cgaacagcac ctggtcttgc gcgagctcga
     4861 tcgcatagtg atgggtctcg ccgctcgcga tcggtttctc gacgcccgcg ccgacggcga
     4921 gccgcggcga ttcgacgtgc ggacgcgcgc acgcaacgtg cagaaccact ggcagaacca
     4981 gcggcaacca gcgcatcaac tagcaacgtc gcgcagcgtg acgcgctcgt caacgacgtg
     5041 gaacctgcct ttgtcgtctt tcgcgatcgt gcccatcgcg atgttcggat cgtgcgtgaa
     5101 gaacaaccga cccttgcgat ggagcagatc gccgagcagc gtcgccttct cgtcgatcag
     5161 catctcggga taacggtcgt agcccatcgt gatcggcaga tgcacccacg ccttgccggg
     5221 aatcagatcg gcggcaaaca cgaccggacc ggcgggcatc gcgacttcgg tgagcaagag
     5281 gccaggcgtg tggcccgagc tgcgatgaaa gcaaaaccca tcaccgagca gctgcgacgt
     5341 ctcggccgcc gcgagctcga gcctgccgct atcgacaagc agatcggtga gcccaggaat
     5401 gaacgacgcg cgatcgcgtg cgtgcggctg catcgcgcgc tgccacgctt ccgcaccgac
     5461 gacgtacgac gcgttcggga acacgagcga cggctgcttg cccgcttgcc acgcgtcgag
     5521 caggccgccc gcgtggtcga agtgcagatg cgagagcacg acgatgtcga cgtcctcggg
     5581 tcggataccg accgcggcaa gtgactcgag taacacatgg cggtcctcga ccactccgta
     5641 acgctcacgt aacgcaggct cgaagaacgc cccgatgccc gcctcgagca ggatcacgcg
     5701 gccgcggtcc cgtacgatca aacaccgaca ggcgagcggg atgcggttcc ggtcgtccgg
     5761 cggaatccac ttttcccaca tggccttggg cgcattgccg aacatcgcgc cgccgtcgag
     5821 gcgctgagca ttgcccagga cggacgtcag ggttctcacc gaacgtctct accacgatcg
     5881 cgacggggtc tcttgtcccc agcgaagcga caaattttgg catcttctcg cgggcatacg
     5941 gctgtgtttt agtcacgaca tgaggggatt gttagtcgca ttcgtagcag cgctggcgtt
     6001 cacgggatgc ggtccgtcaa cgggtggtga tcacggcgat gacgcgaacg caacgctcac
     6061 gatcgatcca ccgaccagcg agctgttgat cgacaacggc atcctgccga cggaagactt
     6121 cacagcgacg ctgacgtatc ccgatggctt cacgcgcgac gtgacgaacg aagtccactt
     6181 caacgtcgat agcggctacg cgatgttcac cgcgaacacg ctcaccgcgc aggcagccgg
     6241 caagacgcag gtgttcgcgt cgtggaccga caagctcgcg gtcgcgcagc tcatcatcaa
     6301 gctccgcaac gtccgcgtcg atccaggttt gcccgttggc gccgccgact ggttcacgaa
     6361 caaccccgaa gatccgacgc gcgcgccgac gattgtttat ccgccggctg acgtcgtcat
     6421 cccgcgcaac ctcggcgact tcgaagtgca ctggaccgat gcgacgaaca acgtgttcga
     6481 gctctcgctc aagaccgagt tcaccgacgt gcgcgtgatc gtacccggcg gcaatggctc
     6541 gatcgcgaac agctcgtggc aagcgttcct cgccgtcgaa tggatggcgg cggtcagcaa
     6601 cgaaacgacc gtgacgtatc aggtgcgcgg tgtcgatcag aacaacccga ctgtcatcgg
     6661 cagcggcgcg ccgcagatcg tgaagctctc gaacgagcag atgctcggcg gcctctacta
     6721 ctgggcggcg accgcgcaga acgggccgta cggcatcttc cgccacgaca tggcgaagcc
     6781 gggccagccc gcggagcagt tctacacgac cgcgcagacc ggcggccgct gcgtcgcgtg
     6841 ccacgtgctg tcgcgcgacg gcaccaagat ggcggtcacg tacgacggtg gcgacggcat
     6901 ggcgacgctc gtcgatgtcg cgaccaagac gccgcaaacc acgcaacgcg gctggaactt
     6961 cggcgcgttc acgccagacg gcaccaagtt cctcgcgaat cgccagggca cgctgaccgt
     7021 catcgactac acgacgcagg cgacgctcgc cacgatgacg gcgtcgggct acgtcacgca
     7081 tcccgacctg tcggccgacg gcacgcgcct ggtctacgtg cagggcccgg gcggcacgag
     7141 cgactggagc ttcggcggtg gcaagatctt cacgcgcacg ttcgatccgg cgacgaactc
     7201 gttcggtccc gagcagctcc tcgtcgccga cacgaacaac aattactacc cgtcgatctc
     7261 gcccgacggt cagtgggtgc tgttcaaccg cgccgccggc ggtagctcgt acaacaacgg
     7321 caacgcaacg ctgtttgtca ccaaggccga tggcactggc atcccgatcg agctcgcgaa
     7381 gtcgaaccag ggcctcgggc tcacgaactc gtggggacgt tgggcgccgt tccagcagac
     7441 gatcggcgcc gcgaacgaac cgatgtactg ggtcaccgtg tcgagcacgc gcaacttcgg
     7501 cgtccgtctc gtcggcgtga accggccgca gatctggatg acgccgttct tcccgacgaa
     7561 ggccgcgggc gcaaccgatc cgagcaacgc ggcgttccgg ctcccgttcc agaacatcga
     7621 cagcaacaac cacatcgcgc agtggaccga gcaagtcgtc gaggtccagt agcttcgtaa
     7681 ccgctgcgcg cgtcggccga ggggggacgg cgcgcgcagc accacgtctg cgggctaggg
     7741 cgcggtcgtg acgctaccgt cgatcttgtc gggcggtgac agcgtgacgg tgtagaccac
     7801 gccgccgacg atcgcggcgc tgcccgcgac gatcgacgtg atgccccacc atttgcgata
     7861 ccagggcgtc ggcgggtcgt agcgcagcac catttcgcgt ggcttcgtcg catccgccca
     7921 cgtcagcacc tcgttgccac gttgatcgta ggcacgcagg tacagctcga gctgcacgtt
     7981 cttgctcgcc tgtaccgcgg gtaagacgac gcgcttgtct tttgcgagct gtgtatcggc
     8041 tgcgcgccag gtcggctcgc cgcgcgtgcg cacgaacaac gtcgcgcgcg acaggaactg
     8101 cttcggatcg gcgagcacct cgatatcgat cggaatcggc tcgccgacct tctggccgcg
     8161 cgaccagttg acgtcgatcg ccggcgcgcc gcgctgcttt gcctgctcga gcgtcttctc
     8221 gaagacgagc gtggccttcg gcgacagctt gtacgacagg aagtgcgcgg ggtcgagcgc
     8281 gagcaacatg tcgaacgcgg t
//
LOCUS       DASUFX010000583         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1345174, whole genome shotgun sequence.
ACCESSION   DASUFX010000583 DASUFX010000000
VERSION     DASUFX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345174"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VFV99_23505"
     CDS             <1..1058
                     /locus_tag="VFV99_23505"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5062366.1"
                     /translation="AGSSPEDANRLAVLWAAAQHLAVQAPRAFGEARHWMKISEDAGL
                     RSRPTQAGDVFQRALIVDALCDLRAPFDESFAPVIAHEVAHVMQSRVCDSCGWGYLPE
                     LDELPADCDSLAQVLQMAVRCGEARREDFDRPLATAFGTSHGDGSFETWILPRAAERD
                     AVHQRQAELVERVWGPGADPEVMSNLLYALALWNRQRFDDRIAAGCRYIVSCQRPDGT
                     WPGRWYVGPYYSTYVAVRLLARVRDGVPAVKRTIEALLAGQRPDGAWSRDHSPRGLDT
                     AHAILTLLAARPLEVADAAAIRASVFRGLGALGRLRKPDGSWAADPFLTMRELVWGSS
                     TLTTTYVLKAAALAEAL"
     gene            1058..1252
                     /locus_tag="VFV99_23510"
     CDS             1058..1252
                     /locus_tag="VFV99_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062367.1"
                     /translation="MRTSSYTIYADLPDRPEVIEELERRLELVQATAAATNWGCDKCA
                     VDCNTVCEQYLICSTDVFHR"
     gene            complement(1268..>2274)
                     /locus_tag="VFV99_23515"
     CDS             complement(1268..>2274)
                     /locus_tag="VFV99_23515"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEX5062368.1"
                     /translation="PRLEHALGVATSAPLTIALIGGVAGMAWLVDHVAAGPEVDALLE
                     HFDGALLRHVAVPQWTDRQDLVSGLAGAGVMLAQRGDARARRIAERVLAHFESTAIES
                     TAGVTWRTDPQFLPDQHRAVFPDGMIDLGVAHGVPGIVGMLAQFVETGIEPERSRALL
                     ERGIAWLLGAVPNGCPRFGTSWPIDDGAKRIGWCHGDTGVATVLMHAGRVLGSEQLVT
                     QGLELLRQVIPFLDERGAPDACLCHGAAGLAHAYNLAFQRTGDNEMREQARKWLQHTM
                     RLRSSGHGIAGYQSFKVDPPGRRWEDDATLLTGVVGTALVLLAATNEHEPVWQRLFVM
                     "
BASE COUNT          365 a          776 c          749 g          384 t
ORIGIN      
        1 cagcgggcag ctcgcccgag gacgcgaacc ggctcgccgt gttgtgggcg gccgcgcagc
       61 atctcgccgt tcaggcaccc cgcgcgttcg gcgaggcacg ccactggatg aagatctccg
      121 aggacgcggg gctgcgttcg cgcccgaccc aggcgggcga tgtgttccag cgcgcgctga
      181 tcgtcgatgc gctgtgcgat ctccgcgcac cgttcgacga gagcttcgca ccggtgatcg
      241 ctcacgaggt cgcccacgtc atgcaaagcc gcgtctgcga ctcctgcggc tggggatatc
      301 tacccgagct cgacgagttg ccagccgact gcgactcgct cgcgcaggtc ctgcagatgg
      361 cggtgcgctg cggtgaggcg cgccgcgagg acttcgatcg cccgctcgca accgcattcg
      421 gcacgtcgca tggcgacggg tcgttcgaga cctggatcct gccgcgtgct gccgagcgtg
      481 atgcggtcca ccagcgccag gccgagctcg tcgagcgcgt gtggggtccc ggcgcggatc
      541 ccgaggtgat gtcgaatctg ctctacgcgc tcgcgctgtg gaatcgccaa cggttcgacg
      601 acaggatcgc cgcaggctgc cgctacatcg tcagctgtca gcgtcccgac ggcacctggc
      661 caggccgctg gtatgtcggt ccttactaca gcacctacgt cgccgtgcga ttgctcgcgc
      721 gcgttcgcga tggtgtaccc gcggtgaagc gaacgatcga ggcgctgctc gcaggtcagc
      781 gtcccgatgg cgcgtggtcg cgtgaccatt cgccgcgcgg gctcgatacc gcgcatgcga
      841 ttctcacgct actcgccgcg cgaccgctcg aggtcgcgga tgctgcggcg atccgcgcgt
      901 cggtgttccg cggcctcggc gcgctcggcc ggttgcgcaa gccggatggc agctgggcag
      961 ccgatccgtt cctgacgatg cgcgagctgg tgtggggcag cagcacgttg accaccacct
     1021 acgtcctcaa ggccgcggcg ctcgccgagg cgctgtgatg cgaacctcga gctacacgat
     1081 ctacgccgat ttgcccgatc ggcccgaggt catcgaggag ctcgagcgcc gcctggagct
     1141 cgtacaggcg accgcggcgg cgacgaactg gggctgcgac aagtgtgccg tcgactgcaa
     1201 caccgtctgc gagcagtacc tgatctgctc taccgacgtg ttccatcggt gaccgggcaa
     1261 cggcctgtca catcacgaac agccgctgcc acacaggctc gtgctcgttt gttgccgcga
     1321 gcaagacgag cgcggtgccg accacgcctg tgagcagcgt cgcgtcgtct tcccagcgac
     1381 gccccggagg atcgactttg aacgattggt atccagcgat tccgtgtccc gacgaacgca
     1441 gcctcatcgt gtgctgcaac catttgcggg cttgttcccg catctcgttg tcgcccgtgc
     1501 gctggaacgc caggttgtat gcgtgtgcga gcccggcagc tccgtgacac aaacatgcat
     1561 cgggtgctcc tcgttcgtcg aggaacggga tcacctgtcg tagcagctcg agaccttggg
     1621 tcacgagttg ctcgctgccg agcacgcgac cggcatgcat cagcaccgta gcgacgccgg
     1681 tatctccgtg gcaccaaccg atccgttttg cgccgtcgtc gataggccag ctcgtgccga
     1741 agcgtggaca gccgttcggc accgcgccga gcaaccaggc gatcccgcgt tcgagcagtg
     1801 cgcgactccg ttcgggctcg ataccggtct cgacgaactg cgcgagcatg ccgacgatgc
     1861 ccggcacgcc atgcgcgacg ccgagatcga tcatgccgtc aggaaacaca gctcggtgtt
     1921 ggtcgggaag aaactgcggg tcggtgcgcc acgtgacgcc ggcagtcgat tcgatcgcgg
     1981 tcgactcgaa atgcgcgagc actcgctccg cgatacgccg cgcacgtgca tcgccacgct
     2041 gcgcaagcat cacgccagca ccagcaaggc cactcacgag atcttggcga tcggtccact
     2101 gcggtacagc gacgtgccgc aacagcgcac catcgaagtg ctcgagcaat gcgtccactt
     2161 ctggacccgc tgcgacgtgg tcgacgagcc atgccatgcc ggcgacaccg ccaatcagcg
     2221 cgatcgtcaa cggtgccgag gtggcgaccc caagcgcgtg ctcgagtcgc ggct
//
LOCUS       DASUFX010000584         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1624927, whole genome shotgun sequence.
ACCESSION   DASUFX010000584 DASUFX010000000
VERSION     DASUFX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624927"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2412
                     /gene="rpoB"
                     /locus_tag="VFV99_23520"
     CDS             <1..>2412
                     /gene="rpoB"
                     /locus_tag="VFV99_23520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5062369.1"
                     /translation="SPYRGSWLDFEFDPKDALFTRIDRRRKLPVTVLLRALGYSNEEM
                     LDIFFEKNVFHLGKKDGAELDLVAERLRGETLNFDLRIGDKVIVEAGKRITARHVRSL
                     QESKIKSLEVPDEYLAGRILAHDIVDKKTGEVLASANDELNEAHLDAFRKAGVDKVAT
                     LWVNDLDRGPYISQTLRIDPTKTPLEALVEIYRMMRPGEPPTKEAAQNLFQNLFFSAE
                     RYDLDRVGRMKFNRRVGRKDDKGPGILYDRKYFGERNDETSKGLVKEFGAGSDILDVL
                     KVLIEIRNGRGTVDDIDHLGNRRVRSVGEMAENVFRIGLVRVERAVKERLSLAEAEGL
                     TPQDLINAKPVAAAVKEFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGF
                     EVRDVHPTHYGRVCTIETPEGPNIGLINSLAVYARTNEYGFLETPYRKVNAGKITNHV
                     EYLSAIDEGEYVIAQANSPLNKDGTFSEDFVSCRFHGESELRPPAEVQYMDVSPMQTV
                     SVAAALVPFLEHDDANRALMGANMQRQAVPTLRAQTPLVGTGIERAVARDSGVTVAAK
                     RGGVIDQVDAGRIVVRVNEAEIGGDDAGVDIYTLTKYTRSNQNTNLNQRPLVNVGDIV
                     AIGDVLADGSSTDLGELALGQNMLVAFMPWNGYNFEDSILLSERVVEEDRYTTIHIEE
                     LTCVARDTKLGPEEISADIPNVGEQALARLDESGVVYIGAEVKAGDILVGKVTPKGES
                     QLTPEEKLLRAIFGEKASDVKDSSLRVPPGMDGTVIDVTVFTRDGIEKDKRARQIEEM
                     EIKRI"
BASE COUNT          476 a          769 c          775 g          392 t
ORIGIN      
        1 tgagtcccta ccgcggctcg tggctggact ttgaattcga tccgaaggac gcgctgttca
       61 cccgcatcga ccgtcgccgc aaattgccgg tgaccgtgct gctgcgcgcg ctcggctatt
      121 cgaacgaaga gatgctcgac atcttcttcg agaagaacgt gttccatctc ggcaagaagg
      181 acggcgccga gctcgacctc gtggccgaac gcctgcgcgg cgagaccttg aacttcgatc
      241 ttcgcatcgg cgacaaggtc atcgtcgaag ccggcaagcg catcaccgcg cgccacgtgc
      301 gctcgctgca ggaatcgaag atcaagtcgc tggaagtgcc cgacgaatac ctcgccggcc
      361 gcatcctcgc gcacgacatc gtcgacaaga agaccggcga agtgctggct tcggccaacg
      421 acgagctcaa cgaagcgcat ctcgatgcgt tccgcaaggc cggcgtcgac aaggtcgcca
      481 cgctgtgggt caacgatctc gatcgcggtc cgtacatttc gcagacgctg cgcatcgatc
      541 cgaccaagac gccgctcgag gcgctggtcg agatctaccg catgatgcgt ccgggcgagc
      601 cgccgaccaa ggaagcggcg cagaacctgt tccagaacct gttcttctcg gccgagcgct
      661 acgatcttga tcgcgtcggt cgcatgaagt tcaatcgccg tgtcggccgc aaggacgaca
      721 agggtccggg catcctgtac gaccgcaagt atttcggcga gcgcaacgac gagacctcga
      781 aaggcctggt caaggaattc ggcgcgggtt cggacatcct cgacgtgctg aaggtgctga
      841 tcgagatccg caacggccgc ggcacggtcg atgacatcga tcacctcggc aaccgtcgcg
      901 tgcgttcggt cggcgaaatg gccgagaacg tgttccgcat cggtctcgtg cgcgtcgagc
      961 gcgcggtcaa ggagcgcctg tcgctcgcgg aagccgaagg tttgacgccg caggacctga
     1021 tcaacgcgaa gcccgtcgcg gccgccgtga aggaattctt cggctcctcg cagctttcgc
     1081 agttcatgga ccagaacaat ccgctcagcg aagtcacgca caagcgtcgc gtgtcggcgc
     1141 tgggtccggg cgggctcacg cgcgagcgcg cgggcttcga agtgcgcgac gttcatccga
     1201 cgcactacgg gcgcgtgtgc acgatcgaaa cgccggaagg tccgaacatc ggcctgatca
     1261 attcgctcgc ggtctacgcg cgcacgaacg agtacggctt cctcgagaca ccgtaccgca
     1321 aggtcaatgc cggcaagatc accaaccacg tcgaatacct ctcggccatc gacgagggcg
     1381 aatacgtgat cgcgcaggcg aactcgccgt tgaacaagga cggcacgttc tccgaagatt
     1441 tcgtgtcgtg ccgcttccac ggcgaatccg agctgcgtcc gccggcggaa gtccagtaca
     1501 tggacgtctc gccgatgcag accgtgtcgg tcgcggccgc gctcgtgccg ttcctcgagc
     1561 acgacgatgc gaaccgcgcg ctgatgggcg cgaacatgca gcgccaggcc gtgccgacgc
     1621 tgcgtgcgca gacgccgctg gtcggaaccg gcatcgagcg cgcggtcgcg cgcgactcgg
     1681 gcgtgaccgt tgccgccaag cgcggcggcg tgatcgacca ggtcgatgcg ggccgtatcg
     1741 tcgtgcgcgt caacgaagcc gagatcggcg gtgatgatgc cggcgtcgat atttatacgc
     1801 tgacgaagta cacgcgctcg aatcagaaca cgaacctcaa ccagcgtccg ctcgtcaatg
     1861 tcggcgacat cgtcgcgatc ggcgacgtgc tggccgacgg atcctcgacg gacctcggcg
     1921 agctcgcgct cggccagaac atgctggtcg cgttcatgcc gtggaacggc tacaacttcg
     1981 aggactcgat cctgctttcc gagcgcgtcg tcgaggaaga ccgctacacg acgatccaca
     2041 tcgaagagct gacctgcgtc gcgcgcgata ccaagctcgg tccggaagaa atctcggccg
     2101 acatcccgaa cgtcggcgaa caggcgctcg cgcgtctcga cgaatcgggc gtcgtgtaca
     2161 tcggcgccga ggtgaaggcg ggcgacatcc tggtcggcaa ggtcacgccg aagggcgaat
     2221 cgcagctgac gccggaagag aaactgctcc gcgcgatctt cggcgagaag gcgtcggacg
     2281 tgaaggattc cagcttgcgc gttccgccgg gcatggatgg caccgtcatc gacgtcaccg
     2341 tgttcacgcg cgacggcatc gagaaggaca agcgtgcgcg ccagatcgaa gagatggaga
     2401 tcaagcgcat ca
//
LOCUS       DASUFX010000585         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1901178, whole genome shotgun sequence.
ACCESSION   DASUFX010000585 DASUFX010000000
VERSION     DASUFX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901178"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFV99_23525"
     CDS             complement(<1..490)
                     /locus_tag="VFV99_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062370.1"
                     /translation="MRRLVVALGLAHASVASGDATVTVTLNQQGERLASDLGLSVPDL
                     ISTAEARIDELYRVSRIEELLRAVGNTAAFAQRGLGADYDLDAGDIFVGAAAAGVHGD
                     VAIGTTNTLLGGSIVNFSLLAGANLQRWHHARWTVFASGFYEATTIHGLEGHLLTLGA
                     HTQ"
     gene            594..2204
                     /locus_tag="VFV99_23530"
     CDS             594..2204
                     /locus_tag="VFV99_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062371.1"
                     /translation="MRWSLLLLLVALPRLAFAEPKVAVAPLDDDDGKVAEIVSDAVAE
                     RAKLTKPARVESAMKSLGVSVLSPKSLKKLRTKLDVDVVIYGSVERDGDTKRVSLTFA
                     GSNKSKPKLELEAKNTKQLRKELAGKLAKRIDSAMEGGGGDDDEEEEDDARAKREQAK
                     REEERKQREEEDRKKEEEKRKKEEEEAEAKRKKEDERRKKDDDEREARRKKKDDDERE
                     ARRKKRGDDDEEDGRRRKKRGDDDEEDGRRKKRVADEDDEDSGRARKRLGDEDEDSGD
                     DEDKPRRKKRKQKRHVLTQAALWLDGGGAVARRTLTYAATGMMRPPPVGTAAAAGRIE
                     GEVYPASFSSLKGGAAGFGISAILGYTFGLGIAVPGTQKTAAIKNGHYSIGARYRFVF
                     GEHSVALGVSYWRRYFMADRTGLMNPDQLDMPDVDYTAIAPGVSARIAATPTIAAFGS
                     LDLPLMLDSGPIQKPASYGSAKILAFDLRAGAQVMVASNIALQFAAEIDQIGLSFTGQ
                     AGSKASTRMVTAATDRSIGLAATLGVTY"
BASE COUNT          490 a          726 c          815 g          328 t
ORIGIN      
        1 actgggtgtg ggcgccgagc gtgagcaaat gaccttcgag gccgtggatc gtggtcgcct
       61 cgtagaaccc actcgcaaac accgtccacc gcgcgtggtg ccagcgctgg aggttcgcgc
      121 cggccagcag cgagaagttg acgatcgagc caccgagtag cgtgttggtc gtgccgatcg
      181 cgacgtcgcc gtgcacgccg gcggcagccg cgcctacgaa aatgtctcct gcatcgaggt
      241 cgtagtccgc accgagcccg cgctgcgcga acgcggctgt gttgccaacc gcgcgcaaca
      301 gctcttcgat ccgtgacacc cgatacagct cgtcgatgcg agcctcggcg gtcgagatca
      361 aatccgggac actgagcccg aggtccgacg caaggcgttc gccctgctgg ttcagggtga
      421 ctgtgacggt tgcatcacca gaggcgaccg acgcatgcgc aagaccgagc gctacaacca
      481 gtcgtcgcat catgtagagc ctactgccgt gtaatgggtc tgcaatcgct cgcaggcgct
      541 atcaatcgat tgcaggctgc tcgggaggcg gtggtcgcgc tattctcggt ggggtgcgtt
      601 ggtcgctcct gctgttgctc gtcgcgctgc cgcgactggc gtttgcggag cccaaggttg
      661 cggtcgcgcc gctcgatgat gacgacggca aagtcgccga gatcgtgagc gatgcagtcg
      721 ccgagcgcgc gaagctgacg aagcccgcgc gcgtcgaaag cgcgatgaag agcttgggcg
      781 tctcggtgct cagcccgaag tcgctgaaga aactgcgcac caagctcgac gtcgatgtcg
      841 tgatctacgg cagcgtggaa cgtgatggcg acaccaagcg cgtgtcgctg acgttcgcgg
      901 gcagcaacaa gtcgaagccg aagctcgagc tcgaggcgaa gaacaccaag cagctgcgca
      961 aggagctcgc tggcaagctc gcgaagcgga tcgacagcgc gatggaaggt ggcgggggcg
     1021 acgacgacga ggaagaggaa gacgacgcgc gcgccaaacg cgaacaggcg aagcgcgaag
     1081 aagagcgcaa gcagcgcgaa gaagaagacc gcaagaagga agaggagaag cgcaagaagg
     1141 aagaggaaga agcggaggcg aagcgcaaga aggaagacga gcgtcgcaag aaggacgacg
     1201 acgaacgcga agcgcgccgc aagaagaagg atgacgacga gcgcgaagcg cgccgcaaga
     1261 agcgcggcga tgacgacgaa gaagatggcc gtcggcgcaa gaagcgcggc gatgacgacg
     1321 aagaagatgg ccgccgcaag aagcgcgttg ccgacgagga cgatgaagac agcggccgtg
     1381 ctcgcaagcg cctcggcgac gaggacgaag acagcggcga cgacgaagac aagccgcgcc
     1441 gcaagaagcg caagcagaag cgccacgtgc tcacgcaggc ggcgctgtgg ctcgacggtg
     1501 gcggcgcagt cgctcgccgc acgctgacgt acgcggcgac cggcatgatg cggccaccgc
     1561 cggtcggtac cgcggctgcg gccggccgga tcgaaggcga ggtctatccg gcgtcgttct
     1621 cgagcttgaa gggcggcgcc gcaggcttcg gcatcagcgc gatcctcggc tacacgttcg
     1681 gcctcggcat cgcagtgccg ggcacgcaga agacggcggc gatcaaaaac ggtcactact
     1741 cgatcggcgc gcgctatcgc ttcgtgttcg gcgagcactc ggtcgcgctc ggcgtcagct
     1801 actggcggcg ctacttcatg gccgatcgga ccggcctcat gaacccagac cagctcgata
     1861 tgcccgatgt cgattacacg gcgatcgcac cgggcgtctc ggcgcggatc gcagcgacac
     1921 cgacgatcgc cgcgttcggc tcgctcgatc ttccgctcat gctcgacagc ggaccgatcc
     1981 agaagccggc gagctacggg tcggcgaaga tcctcgcatt cgaccttcgc gcaggcgcgc
     2041 aggtgatggt cgcgagcaac atcgcgctcc agttcgctgc cgagatcgat cagatcggcc
     2101 tgtcgttcac cggtcaggcg ggcagcaaag catcgacgcg catggtgacg gcggcgaccg
     2161 atcgctcgat cggcctcgct gccacgctcg gcgtcacgta ttagctcgcc gctgcgagaa
     2221 acgcctttgc ggcagccttg ccgcccttgt cgcgaatcac gagccggcgc accgactcgc
     2281 ggcgcatctt catcgagatc atgccgaaga agccggtcgt gatcttgaac gtctcgagct
     2341 cggcgatcgg aaacaccca
//
LOCUS       DASUFX010000586         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_235297,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000586 DASUFX010000000
VERSION     DASUFX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235297"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2957)
                     /locus_tag="VFV99_23535"
     CDS             complement(<1..2957)
                     /locus_tag="VFV99_23535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062372.1"
                     /translation="MRTKQLAIALALALGLPSSAAWAQKQAQEVDVQRGPAAELYIRK
                     RPSAPEAPVLSADLKTLLASTEKRRDDKRLEAIGLLRSFLGSNPSGEARADGMFKLAE
                     LLWEESRRLYLIRMDDYSRALEKCSQKKGECDMPKEPRIQLQEAAALYTELHDKFPKF
                     RRMDLVTYLIGFAAKEDGREDDAMAKFQEVIDRFPQSPLFGDAWMMVGEHWFATSQWQ
                     KSIDAYAHIPDSAATSDLATFKTAWCYWKLGQSEQAAKDFKRVLDKAVEAERTGTAAQ
                     RRRSAGLRDEALEYLVVVFTEDRSIGAQEVFDFLASIGGERYSRDVMIKVAESYAGQA
                     EWERSNEAYRFLIKMEPGGIKAAEYQREIVQNWTSALDVERAQDEIKVLLDNYGPQTA
                     WAKEQKNRDALARSLETTEQLVRTTATNLHGEAQRREKIARIPKQTGCATTPIGNKDL
                     QTLYTKAADAYDLYLTAFAQSKVSAEKTIELRYYRADILCFKLGKVEPAGDEYLAVGK
                     SAPVGKYHKDALLNAMAAFEAARPKDTVGRRKLYDVDKKFGEAIDLYATLFPADPTLV
                     GVIFKNGQMFYDYGEYDEAIKRFGVIVTKYPKDPNAGPAGDRILSALNKAQDYENIED
                     WARKLKSAPSFASKDQQDRLSRLIVESIQKSGDKYADAGKYEQAAKFYLRVPKETGDA
                     KVAAQAMMNAGVMYEKAKQPEDAADVYIDLAQKYATSSKDTASKAAFAAGQVYEKAMF
                     YDRAAKSYELVYDKFGSDKNDPKVADSLFNAGVLRQAIGQNDRAIAHYKKYKDQFASR
                     KDAPDVAFNIGVVYQDAGQEGPAYTAFADYTRTYRSSGRRVIEAHTRAGLMSLKLGQL
                     KRAKEDFAAAQALYKHANGKEKAEGKTWAAQARYNEGELIFREYEKVTLDVKPSQLQK
                     ALTSKSKLLAEAEKVYGSVVDYEDLKWATAALYRTAQVYDGFAESLINAPTPKGLPQD
                     QADAYRQTLDMYVV"
     gene            complement(2954..>3845)
                     /locus_tag="VFV99_23540"
     CDS             complement(2954..>3845)
                     /locus_tag="VFV99_23540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062373.1"
                     /translation="RKDPDQRRALFAKAITRFREVKQTGEVEGLKPGTEKPATAFDDV
                     VALLRLDPKFLRLNDAVTGANELANSAPQVVRQWQNLAANVKETKVGKVSGQASLEEE
                     QLADANAIVEDLRRLQKQISTQRGEIARAKREKLMPADEARKELDHLDDLSAKVEVAL
                     ANGLATADKAAQAVAAQAPGGIKPMIQADIAEARRLDKASHALARKLAEAGEKLAQSA
                     TDRLYDDTRKVLDKAKLGKIDAVIGQKRKLDIEVQDLAAGRFPEELIGRMWNASMIAD
                     DEEYWPFQGEYWADEYGGWR"
BASE COUNT          515 a         1271 c         1227 g          832 t
ORIGIN      
        1 acgacgtaca tatcgagcgt ctgccggtag gcatcggcct ggtcctgcgg caggcccttc
       61 ggcgtcggcg cgttgatcag cgactcggcg aacccgtcgt agacctgcgc cgtgcgatac
      121 agcgccgcgg tggcccactt cagatcctcg tagtcgacga ccgaaccgta gaccttctcg
      181 gcctcggcga gcagcttgct cttcgacgtg agcgctttct gcagttgcga cggcttcacg
      241 tcgagcgtga ccttctcgta ctcgcggaag atgagctcgc cctcgttgta gcgagcctgc
      301 gctgcccacg ttttgccttc ggccttctcc ttgccattgg cgtgcttgta gagcgcctgc
      361 gccgcggcga agtcctcctt cgcgcgcttg agctgaccga gcttgagcga catcagaccg
      421 gcgcgcgtgt gcgcctcgat cacgcggcgg ccgctcgatc gatacgtgcg cgtgtagtcg
      481 gcgaacgcgg tgtacgcggg gccttcttgc cctgcgtcct gatacacgac gccgatgttg
      541 aacgcgacgt cgggcgcgtc tttgcgcgac gcgaactggt ccttgtactt cttgtagtgc
      601 gcgatcgcgc gatcgttctg gccgatcgcc tgccgcagca cgcccgcgtt gaacagcgag
      661 tcggcgacct tcggatcgtt cttgtcgctg ccgaacttgt cgtagacgag ctcgtacgac
      721 ttcgcggcgc ggtcgtagaa catcgccttt tcgtaaacct ggccggcggc aaacgcagcc
      781 ttcgaggcgg tgtccttgga gcttgtcgcg tacttctgcg cgaggtcgat gtaaacgtcg
      841 gcggcgtcct cgggctgctt cgccttttcg tacatcacgc ccgcgttcat catcgcctgc
      901 gcggcgacct tcgcgtcgcc ggtctccttc ggcacgcgca ggtagaactt cgcggcctgc
      961 tcgtacttgc cggcgtcggc gtacttgtcg ccgctcttct ggatcgactc gacgatcaga
     1021 cgcgacaagc gatcctgctg gtccttgctc gcaaacgacg gcgcgctctt cagcttgcgc
     1081 gcccagtctt cgatgttctc gtagtcctgc gccttgttga gcgcggacag gatgcgatcg
     1141 ccggcgggac ccgcgttcgg atccttcgga tacttcgtca cgatcacgcc aaagcgtttg
     1201 atcgcctcgt cgtactcgcc gtagtcgtag aacatctggc cgttcttgaa gatgacgccg
     1261 acgagcgtcg ggtcagccgg aaacagcgtc gcgtagaggt cgatcgcttc gccgaacttc
     1321 ttgtcgacgt cgtacagctt gcgccgaccg acggtgtcct tcgggcgcgc ggcctcgaac
     1381 gcggccatcg cgttgagcag cgcatccttg tggtacttgc cgacgggcgc gctcttgccg
     1441 accgcgaggt actcgtcgcc ggcgggctcg accttgccga gcttgaagca cagaatgtcg
     1501 gcgcggtagt accgcagctc gatcgtcttc tcggcagaca ccttcgactg cgcgaacgcg
     1561 gtgaggtaga ggtcgtaggc gtcggcagcc ttcgtgtaga gggtttgcag gtccttgttg
     1621 ccgatcggcg tcgtcgcgca accggtctgc ttcgggatcc tcgcgatctt ctcgcggcgc
     1681 tgcgcctcgc cgtgcaggtt cgtcgcggtc gtgcggacga gctgttcggt cgtctcgagc
     1741 gagcgggcga gggcgtcgcg gttcttctgt tccttggccc acgcggtctg cgggccatag
     1801 ttgtcgagca gcaccttgat ctcgtcctgc gcgcgttcga cgtcgagagc gctcgtccag
     1861 ttctggacga tctcgcgctg gtactcggct gccttgatgc cgccgggctc catcttgatc
     1921 aggaaccgat aggcctcgtt gctgcgctcc cactcggcct ggccggcgta gctctcggca
     1981 accttgatca tgacgtcgcg cgagtaacgc tcgccgccga tcgacgcgag gaagtcgaac
     2041 acttcctggg caccgatcga gcgatcttcc gtgaacacga cgacgaggta ctcgagtgct
     2101 tcgtcgcgca ggccggcgct gcggcggcgc tgtgcggccg taccggtacg ctcggcctcc
     2161 acggccttgt cgagcacgcg cttgaagtcc ttcgcggctt gctcactctg gccgagcttc
     2221 cagtaacacc acgcggtctt gaatgtcgcg aggtcgctcg tcgctgcgct gtcggggatg
     2281 tgcgcgtacg cgtcgatcga cttctgccac tgcgaggtcg cgaaccagtg ctcgccgacc
     2341 atcatccacg cgtcgccgaa tagcggtgat tgcgggaacc gatcgatcac ttcctggaac
     2401 ttcgccatcg catcgtcttc gcggccgtct tccttggcgg cgaagccgat caggtacgtg
     2461 acgagatcca tgcggcggaa cttcgggaac ttgtcgtgta gctcggtgta gagcgcggcc
     2521 gcttcctgga gctggatgcg cggctccttc ggcatgtcgc actcgccctt tttctgcgaa
     2581 cacttctcga gcgcgcggct gtagtcgtcc atcctgatca ggtagagccg gcgcgattcc
     2641 tcccacagca gctcggcgag cttgaacatg ccgtccgcgc gcgcttcgcc actcgggttc
     2701 gaaccgagga acgagcgcaa taggccgatg gcttcgaggc gcttgtcgtc gcgccttttc
     2761 tcggtcgacg cgagcagcgt cttgagatcg gccgacagca ccggtgcttc aggcgcggac
     2821 gggcgcttgc ggatgtagag ctcggccgcg ggaccgcgtt gaacgtcgac ctcctgggcc
     2881 tgcttctgcg cccatgcggc gctcgaggga aggccgagtg caagcgccag cgcgatcgcg
     2941 agctgcttcg tcctcatcgc cagcccccgt attcgtccgc ccagtactcg ccctggaacg
     3001 gccagtactc ctcgtcgtcg gcgatcatgc tcgcgttcca catccggccg atcagctcct
     3061 cggggaagcg acctgccgcg aggtcctgca cctcgatgtc gagcttgcgc ttctggccga
     3121 tgacggcgtc gatcttgccg agcttcgctt tgtcgagaac cttgcgcgtg tcgtcgtaga
     3181 ggcggtcggt cgccgattgc gcgagcttct cgccggcctc ggcgagcttg cgcgcgagcg
     3241 cgtgcgatgc cttgtcgagc cggcgcgcct ccgcgatgtc ggcctggatc atcggcttga
     3301 taccgccggg tgcttgcgcc gcgacggcct gcgctgcttt gtctgcggtc gcgagcccgt
     3361 tcgccagcgc gacctcgacc ttcgccgaca gatcgtcgag gtggtcgagc tccttcctgg
     3421 cctcgtccgc gggcatcagc ttctcgcgct tcgcgcgcgc gatttcgccg cgctgcgtgc
     3481 tgatctgttt ttgcagccgg cgcaggtcct cgacgatcgc gtttgcgtca gcgagttgtt
     3541 cttcttcgag cgatgcctgg ccgctgacct tgccgacctt cgtctccttc acgttcgctg
     3601 cgaggttttg ccactggcgc acgacctgcg gcgcactgtt cgcgagctcg ttggcgccgg
     3661 tcaccgcgtc gttgagtcgc aagaacttgg gatcgaggcg gagcagggcg acgacatcgt
     3721 cgaacgcggt cgccggtttt tccgttcctg gcttgaggcc ttcgacctca ccggtctgct
     3781 tcacctcgcg aaagcgcgtg atcgccttcg cgaacaacgc gcggcgctga tctggatctt
     3841 ttcgc
//
LOCUS       DASUFX010000587         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1068169, whole genome shotgun sequence.
ACCESSION   DASUFX010000587 DASUFX010000000
VERSION     DASUFX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068169"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFV99_23545"
     CDS             complement(<1..597)
                     /locus_tag="VFV99_23545"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5062374.1"
                     /translation="MDEIILHQPPHRSWGAPNLSPFCAKLECYLRIADVPYKPHRAEF
                     RKAPKGKIPFVEIDGKHMGDSQLIIEELERRLAAEGKEPLDRGMSAQDVAVARMIRRT
                     IEEGFYFVGLYTRWNTDDGYALIRDEFKKIVPGFIIPIIRRAQNKKLKAQGTGRHTLD
                     EAMAMGVADVDAIAALLGDKPFMLGDKPRVIDCTVFGFL"
     gene            752..3187
                     /locus_tag="VFV99_23550"
     CDS             752..3187
                     /locus_tag="VFV99_23550"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062375.1"
                     /translation="MAHANVAAEPQHLVQFYDDVDILAAQVGAFLHGALHASGSAIAV
                     ARPEVLGKIELQLAKAGHDAGSLQRGERLLLIDAHDMLERLLVDGIPSKERFDALIRP
                     VVQRMTACSPVRAYGEIVDILWSAGNRPGVVALETCWSELLEEFPLRLLCGYQLSRFD
                     RDVDGFELVCDHHDAVGQTQHQHPSAVTASRVLVQLEQRARVLEFEVGRRTHLERRMH
                     HLLELTGQLAAAPDRHAIGRLAVEHGMEALGAVDGVMWLVPSVGTHLELLAATGNHHS
                     SSTLDHYRLLPMDEDIPAIHAIRSGEPLFFGSLDEYAERFPASRARLEDILTRGQRAF
                     AVLPVAVDNKPLGVIVYTFDHERVFSAADRAFKSILARQCGLALARVQQQEQERALRE
                     AAERAALAEKAARKEIELLYDLIASLNQLDDLQAVYDLALQTVQRGARSDRAAILLFD
                     PDGVMRFKTAHGLSETYQRAVEGHSPWKPDAVNPQPIVVNDTETDDAWALYRDVFRAE
                     GIRALAFVPITHGRQLIGKFMLYRDDARRFELGDLQFTATVAIHVAQAVARKRNEIEL
                     ARAYSVERDAHLEAEEATRAREEILSVVSHDLRNPLGTILMGATSLLNVDLGDRSHRI
                     RSMAERIHRQAERMARLIEDLVDFAGIQAGQLAIDRKLHNPEEILAATRDIFGPMAQE
                     RGLRFESCTTPGLPAIDCDCERAVQVMANLVANALKVTPKGGAIAIGAEPKNSEVVFY
                     VRDTGPGIADDELPQLFERYWRSKHSQYKGAGLGLSIARGIVDAHGGRIWAESQLGRG
                     ATFYFSLASLRAN"
     gene            3356..5614
                     /locus_tag="VFV99_23555"
     CDS             3356..5614
                     /locus_tag="VFV99_23555"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5062376.1"
                     /translation="MVVEGHALDDVTLAGYVLGPELGTAPVVVIIGGITASPFPFGDA
                     ASGTEPWWPALNAPELIDPTRYTVLAPCWPGNGSTWRGFDDGPLPPLSALGLADLIAA
                     WLDGIGCAAPIAFIGASLGGLVGVALAVRHPDRVARLITISAGLRPDGWGTATRHLQR
                     ELVRDGQRNGDVTTGMIRARQLGMLTYRGREELDTRFGALGPDLEHPPVAAYLQHHGE
                     RFAARFPVRTFLLLSEAIDRARIGTDIASVRAELARVTAEVLIVGVPGDLLFPFALQH
                     ELYRELQAAGATASLWKLDSEYGHDAFLAEQDGLAALLRDSGFFTKVAAEPLPRFHGV
                     GTKPLREIRIGLVGCGVVGSGVLELIDRQRAALAERYGVKFRVTHIAVRDLAKQRTAL
                     ADNIPRTARALDLIAAPDVDVIVEVAGGDGVESVVKAALAAGKPVVTANKALLAKQLA
                     ALGVLAQRTETPLLCEAAAAAAIPIIRHLSQRADEVESLMGIVNGTCNYIITRLEQDE
                     LPLDRAIAEAQSLGLAEADPSADLDGHDAAAKLSILAYRAFGTWIPPHELPVRGIGEL
                     WPADCDLAESMGFRIRLIARAARTPAGFTAGVEPVLLPDWHLLASVEEEYNAVYMKTT
                     QSGDLSLFGKGAGALPTASAVLGDLIDLAQDNSVQWPTPRAVALVAPPARRHYVRVSA
                     ETHPGVARRVDSMVRRAGLAVVNRATRGEGQITHYGFLLSRSDDAIVRSVVDQLRELG
                     RVEQTVWLGTAE"
     gene            5611..>6883
                     /locus_tag="VFV99_23560"
     CDS             5611..>6883
                     /locus_tag="VFV99_23560"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5062377.1"
                     /translation="MSVAAWLDPAHVPAERPPLSAMGSATRELHESINANAPYLHPER
                     RTIQRDLGDSSNGVAWQEAKAAFTLTGDGAWSELPHLYARYGTPGTTELIGALRALEH
                     AKCGFVTDCGMQAVALVADVSIGHGSHAIAMRQVYNKTRAFFEATAKRVGATVTLVDD
                     GDLAALAAALRPETRLVFGETYTNPLGRAQDIPAIIELVRRVPGARLVIDSTIATPWG
                     VRTPLLDQGVDVVTGSLTKALGGQDAALGGYIVTNHAELGNQIMDLIAMRGGILDDER
                     ARRVVGHLPTAERLHARRCETAAKVAAFLAAHPRIERVFHPSRPDHPDAAIIARDYVR
                     TGSLLAFRVKDRDEAGHRKVADVLSSCGVLRYALSFDGLVTKINHHRSVSEYFTPPET
                     VARLGIDRLVRLGIGIEDADDLIACLNWTLHN"
BASE COUNT         1136 a         2347 c         2231 g         1169 t
ORIGIN      
        1 gaggaagcca aacacggtgc agtcgatcac gcgcggcttg tcgccgagca tgaacggctt
       61 gtcgccgagc aaggcagcga tcgcgtcgac gtcagcgacg cccatcgcca tcgcttcgtc
      121 gagcgtgtga cggccggtgc cctgcgcctt gagcttcttg ttctgtgcgc ggcgaatgat
      181 cgggatgatg aatccgggga cgatcttctt gaactcgtcg cgtatgagtg cgtagccgtc
      241 gtcggtattc cagcgcgtgt agagcccgac gaaatagaag ccttcttcga tcgtgcggcg
      301 gatcatgcgt gcgaccgcga catcctgcgc agacatcccg cgatcgagcg gctccttgcc
      361 ttccgcagca agtcggcgct cgagctcctc gatgatcagc tgcgagtcgc ccatgtgctt
      421 gccatcgatc tcgacgaacg gaatcttgcc cttcggagcc ttccgaaact cggcgcgatg
      481 cggcttgtag ggaacgtcgg cgatgcgcag gtagcactcg agcttcgcgc agaacgggct
      541 gaggttcggc gcgccccacg accgatgcgg aggttgatga aggatgattt cgtccacggg
      601 gcgacggtgc cacggtcccc aataagatcg cgaggctcga gcgagaagaa cgatgcgggt
      661 tcgcacgctg tggcattgcc gcacaccgcc tcgagagcgt cggtcaactc gcgcaacgga
      721 ttttgccggc acagtgctat cagtggactt catggcacac gcgaacgtcg ctgctgagcc
      781 acaacacctc gtgcagttct acgacgacgt ggacatcctc gccgcgcaag tcggcgcgtt
      841 cctgcacggt gcgctgcacg caagtggctc tgcgatcgca gtcgcccgtc ccgaggtcct
      901 cggcaagatc gagctgcaac tcgcaaaggc aggacacgac gccggctcgt tgcaacgcgg
      961 cgaacgtctg ctgctgatcg acgcacacga catgctcgag cggttactgg tcgatggcat
     1021 cccgagcaaa gaacgctttg acgcgctgat ccgcccggtc gtccaacgca tgacggcatg
     1081 cagtccagtg cgtgcctacg gcgagattgt cgacattctg tggtccgctg ggaatcggcc
     1141 tggcgtggtt gcactcgaaa cgtgctggtc ggagctactc gaagagtttc cgctgcggct
     1201 tctgtgcggc taccagttgt cccggttcga tcgcgacgtc gacggcttcg agctggtgtg
     1261 cgatcaccac gacgccgtcg gccagacgca acaccagcac ccctccgcgg tcacggcgtc
     1321 gcgcgtactc gtgcagctcg agcaacgcgc gcgtgtgctc gagttcgagg tgggtcgtcg
     1381 cacgcacctc gagcggcgca tgcaccactt gctcgagctc accggccagc tcgcggcagc
     1441 acctgatcgc cacgcgatcg gacgactcgc cgtcgaacac ggcatggaag cactcggcgc
     1501 ggtcgacggt gtgatgtggc tcgttccgag cgtgggcaca cacctcgagt tgctcgcggc
     1561 aaccggcaac catcacagct cgagcacact cgatcactat cgcctgctgc cgatggacga
     1621 ggacattccg gcgattcacg cgatccgcag cggggagccg ttgttcttcg gctcgctcga
     1681 tgaatatgcg gagcggttcc cggcgtcgcg cgctcgcttg gaggacatac tgacccgcgg
     1741 gcagcgtgcg ttcgcggtct tgccggtcgc cgtcgacaac aaaccgctcg gcgtgatcgt
     1801 ctatacgttc gatcacgagc gagtgttctc ggcggcggat cgcgcgttca agtcaatcct
     1861 tgctcgccaa tgcggtctcg cactcgcacg cgtgcagcag caggagcagg aacgagcgct
     1921 gagagaagcg gctgaacgcg cggcgctcgc cgagaaagcg gcgcgcaaag agatcgagct
     1981 gctctacgat ctgatcgcgt cgctcaacca gctcgatgat ttgcaagccg tctacgatct
     2041 cgcattgcaa accgtgcagc gcggcgcgcg ttcggatcgc gcagcgatct tgctcttcga
     2101 tcccgacggc gtcatgcgct tcaagacggc gcacggcctg tcggagacgt accagcgcgc
     2161 cgtcgagggc cattcgccgt ggaagccgga tgcggtcaat ccacaaccga tcgtggtgaa
     2221 cgacaccgag accgatgacg cgtgggcgct ctaccgtgac gtgttccgcg ccgagggaat
     2281 tcgcgccctc gcattcgtgc cgatcacgca cgggcggcag ctcatcggca agttcatgct
     2341 ctaccgcgac gacgcgcgcc gcttcgagct cggtgatttg cagttcaccg cgacggtcgc
     2401 gattcacgtc gcgcaagcgg tcgcgcgcaa acgcaacgag atcgagcttg cccgcgcata
     2461 cagtgtcgag cgcgatgccc atctcgaggc cgaggaggca acgcgggccc gcgaggagat
     2521 cctgtcggtc gtctcgcatg acctgcgtaa cccgctcggc acgattctga tgggtgcgac
     2581 atcgctgctc aacgtcgacc tcggtgatcg cagtcatcgt attcgctcga tggccgagcg
     2641 cattcaccgc caggccgagc gaatggcgcg actgatcgaa gacctcgtgg acttcgcggg
     2701 catccaggcg ggtcagctcg cgatcgaccg gaagctgcac aaccccgaag agattctggc
     2761 ggcgacgcgc gacatcttcg gaccgatggc gcaggagcgt ggactgcgtt tcgagtcgtg
     2821 cacgacgccc ggacttcctg cgatcgattg cgactgcgag cgcgcggtcc aggtcatggc
     2881 gaacctcgtc gcgaatgcgc tcaaggtcac gccgaagggc ggcgcgatcg cgatcggcgc
     2941 cgagcccaag aacagcgaag tcgtgttcta cgttcgcgat accgggcccg gtattgcgga
     3001 cgacgagctg ccgcaactat tcgagcgcta ctggcgcagc aagcactcgc agtacaaggg
     3061 cgccgggctc ggcctgtcga tcgcgcgcgg catcgtcgac gcgcacggcg gtcggatctg
     3121 ggccgagagc cagctgggcc gtggcgcgac cttctacttc tccctcgcct cgcttcgcgc
     3181 caactaggct ccgggccagg aggaaccgcg cgggggtagc acccgcagaa atcgcctaac
     3241 tccgcggttg acgaggagct atcggccgtt ccaatatccc ggacaaatgt ccgcgatagc
     3301 gcacgccgcc ccggcccttc cctcaccacg tcgcatcgaa gttcggcgcg acacgatggt
     3361 tgtcgaaggg cacgcgctcg acgacgtcac gctcgcgggc tacgtgcttg ggcccgagct
     3421 cggcaccgcg ccggtcgtcg tcatcatcgg gggcatcacc gcatcgccat ttccgttcgg
     3481 cgatgccgcg agcggcaccg agccatggtg gcctgcgctc aacgcacccg agctgatcga
     3541 tccgacacgg tacaccgtgc tcgcaccgtg ctggccgggc aatggctcga catggcgcgg
     3601 cttcgacgac ggcccgctgc cgccgctgtc tgcactcggt ctcgccgatc tcatcgccgc
     3661 gtggctcgat ggaatcgggt gtgcagcgcc gattgcattc atcggtgcga gcctcggcgg
     3721 gctcgtcggc gtcgcgctcg cagttcgaca tcccgatcgc gtcgcgcgtc tgatcacgat
     3781 ctcggcaggt ctgcgtcccg acggctgggg caccgccacg cgtcacctcc agcgcgagct
     3841 agtgcgcgat ggtcagcgta acggcgacgt cacgaccggc atgatccgcg cgcgtcagct
     3901 cggcatgctg acgtatcgcg gccgcgagga gctcgacacg cgatttggtg cgctcgggcc
     3961 cgatctcgaa catccgccgg tcgccgcgta tctccagcat cacggcgaac gcttcgcggc
     4021 acgctttcca gtgcgcacgt tcctgttgct cagcgaggcc atcgatcgcg cgcgcatcgg
     4081 caccgacatc gcttcggttc gcgccgagct cgcgcgcgta acggcggagg tgttgatcgt
     4141 cggcgtaccc ggcgacctct tgttcccgtt cgcgctgcag cacgagctgt atcgcgagct
     4201 gcaagcggcc ggcgccactg cctcgctgtg gaagctcgac tccgagtacg gacatgacgc
     4261 gttcctcgcc gaacaagacg gtctcgccgc actgctgcgc gatagcgggt tcttcacgaa
     4321 ggtggctgcc gagccgctgc cgcgttttca cggcgtgggc acgaagccgc tgcgcgagat
     4381 ccgcatcggt ctcgtcggct gcggcgtcgt cggcagcggc gtgctcgagc tgatcgatcg
     4441 ccagcgcgcc gcgctcgccg agcgctacgg cgtcaagttc cgcgtcacgc acatcgcggt
     4501 tcgtgatctc gccaagcagc gcaccgcgct cgccgacaat atcccgcgca cggcgcgcgc
     4561 gctcgatctg atcgccgcgc ctgatgtcga cgtgatcgtc gaggttgcgg gtggcgacgg
     4621 agtcgaatct gtcgtcaaag ccgcgctcgc cgcgggtaaa ccggtggtca ccgcgaacaa
     4681 ggcattgctc gcgaagcagc tcgccgcgct cggcgtgctc gcgcaacgca ccgagacccc
     4741 gcttttgtgc gaggccgcgg cggccgctgc tatcccgatc atccggcacc tctcccagcg
     4801 tgccgacgaa gtcgagtcgc tgatgggcat cgtcaacggc acgtgcaact acatcatcac
     4861 gcgcctcgag caggacgagc tgccgctcga ccgcgcgatc gccgaagcac aatcgctcgg
     4921 gctcgctgaa gcagatccgt ccgcggatct cgacggtcac gacgccgccg caaagctctc
     4981 gatcctcgcg tatcgcgcat tcggcacctg gatcccgccg cacgagctgc cagtgcgcgg
     5041 catcggtgag ctatggcccg cggactgcga tctcgcggag tcgatggggt tccgcatccg
     5101 gctgatcgcg cgcgcggcgc gcacgccggc cggcttcacc gccggtgtcg agccggtcct
     5161 gttgcccgac tggcacctgc tcgcgtccgt cgaggaggaa tacaacgcgg tgtacatgaa
     5221 gacgacgcag tccggcgatc tgtcgctgtt cggcaagggt gccggcgcac tgccgaccgc
     5281 gagtgcagtg cttggcgatc tgatcgatct cgcacaggac aactcggtgc agtggccgac
     5341 gccgcgtgcc gttgcgctcg tcgcgccgcc agcacgccgc cattacgtgc gcgtcagcgc
     5401 tgaaacgcat cccggggtcg cgcgccgcgt ggactcgatg gtccggcgcg cgggacttgc
     5461 cgtcgtcaat cgcgcgacgc gaggcgaagg tcagatcacg cactacggct tcttgctctc
     5521 gcgatcggac gacgcgatcg tccgctcggt cgtcgaccag ctccgcgagc tcggccgcgt
     5581 cgagcaaacc gtctggctcg ggacggcaga atgagcgtcg ctgcctggct cgatccggcg
     5641 cacgtgcctg ccgagcgacc gccgctatcc gcgatgggca gcgcgacgcg cgagctgcac
     5701 gaatcgatca acgcgaacgc cccgtatctg catcccgaac gccgcacgat ccagcgcgac
     5761 ctcggcgact cgagcaatgg cgtcgcatgg caggaggcga aggcagcgtt cacgctgacc
     5821 ggcgacggcg cgtggtcgga gctgccgcac ctctacgctc gctatggcac gcccggcacg
     5881 accgagctga tcggagcgct gcgcgctctc gagcacgcga agtgcggctt tgtcaccgac
     5941 tgcggcatgc aggcagtcgc gctcgtcgcc gatgtctcga tcggccacgg ctcgcacgcg
     6001 atcgcgatgc ggcaggtcta caacaagacc cgcgcattct tcgaagcgac cgcgaagcgc
     6061 gtcggcgcca cggtcacgct cgtcgatgac ggtgacctcg cggcgctcgc cgctgcgcta
     6121 cgtcccgaaa cccgtctcgt gttcggcgag acatacacga acccgctcgg ccgcgcgcag
     6181 gacattcctg cgatcatcga gctcgtgcga cgcgtgcccg gcgcccgcct cgtgatcgac
     6241 tcgacgatcg cgactccgtg gggtgtccgc acgccattgc tcgatcaagg ggtcgacgtt
     6301 gtcaccggca gcctcacgaa ggcgctcggc ggccaggacg cggcactcgg cggctacatc
     6361 gtcacgaacc acgccgagct cggcaatcag atcatggatt tgatcgcgat gcgcggtgga
     6421 atcctcgacg acgagcgggc acgccgcgtc gtcggccact tgccgaccgc cgagcgcttg
     6481 cacgcgcgtc gctgcgagac cgcggccaag gtcgccgcgt tcctcgccgc ccatccgcgg
     6541 atcgagcgcg tgttccaccc gagccgcccc gatcatcccg atgcggcgat catcgcgcgc
     6601 gactacgtgc gcaccggctc gctgctcgcg ttccgcgtca aggatcgcga cgaagcgggc
     6661 caccgcaaag tcgccgacgt gctgtcgtcg tgtggtgtgc tgcgctacgc gctgtcattc
     6721 gatggcctgg tcaccaagat caaccaccac cgcagcgtct cggagtactt cacgccgccc
     6781 gagaccgtgg cacgcctcgg catcgatcgc ttggtccgcc tcggcatcgg tatcgaggac
     6841 gccgacgatt tgatcgcgtg cctgaactgg acgctccaca act
//
LOCUS       DASUFX010000588         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_513693,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000588 DASUFX010000000
VERSION     DASUFX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513693"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..1105)
                     /locus_tag="VFV99_23565"
     CDS             complement(449..1105)
                     /locus_tag="VFV99_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062378.1"
                     /translation="MRIESTGITIDGARVDLAEAVRRGKAAGQAAVIVTPNAPASLYT
                     GLMAALSAANVPTMRNARRRTASRPSRRNRELSSTFTLVVLPEGIWGTTKRVRYFKTD
                     APTTWEDARDRLAAAKFLDRDALSPNGAGAWRLVTDPARFRADRAETLPGGSHRPRGA
                     ARATDRYTLEGRTILRDGERILYVDRVDLGDSRYAISPHHADLLTERMVRLLNRHGAR
                     "
     gene            complement(1158..>2280)
                     /locus_tag="VFV99_23570"
     CDS             complement(1158..>2280)
                     /locus_tag="VFV99_23570"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5062379.1"
                     /translation="IVEPRLAKQSDTTASATLLPASSSSAPAATLPIRSMRSLAAGPS
                     GDMRPIDPYADATPSLTVAPTRPASTSLVTTPTTSSVPTTDGPITMPSQLVLTSTPIA
                     PTSATGTTCPCPTTPPIGVTAPHATSPATPTPSATPSASFATSPRVRRFDPVFERYRG
                     SVPIEYVRALVERESSGNYLTQTGSALGLMQIVPVVLDDYNKRHGTPYQREHLFDPSI
                     NVAIGCELLRLIIDNYRKHHPRIANLQGNWSNPRFVELLTFGWNAGYSEAGGVGRVAR
                     YLEGLGAVDITMDQVSAHAKLAGASKHLSNPAKLTWCKSVVALYLREPRSGVRPLWSE
                     RSVHVEHLYIFARTRKRCRGRVPDQEQAHERAGFPDRIE"
BASE COUNT          344 a          728 c          819 g          389 t
ORIGIN      
        1 gcccgcggcg gcgacgccgc cgccgagcag gacgaacccg agcgtatcgt cgtcgcgcgt
       61 gctcatagcc gctccaaccg agcgagccac tcgagcgagg gtggtagcag cagcttgatc
      121 cccgccgctt ggagtgccct gagcactccg tgaatgacgc cagtgatcgc atcgcccgtc
      181 gcaccgacct gagcggtacc gaccgcgcga cacgcttcga ctacagtcgg taggtccgca
      241 gcgacgccat cgaccgtgag ccgttcgggg tagatccaga ccttgcattg cccaggggat
      301 cgcgttgggt cggcgttctg gccctgaccc ttcccatcgc cggggctgcg aaagccccag
      361 ccgttgccgc gggagaggag ccaccacgct gtgagcgccg cgccgccgac aagcgcaatg
      421 gtttggccgg tacgatcgcg gtcggccatt agcgtgctcc gtgccggttg aggaggcgaa
      481 ccatccgttc ggtgagcaaa tcggcgtggt gtggagaaat cgcgtagcgc gaatcgccga
      541 ggtccacgcg atcgacgtag agaatccgct cgccgtcgcg caggatcgtg cggccttcga
      601 gcgtgtagcg atcggtcgcg cgtgcggcgc cacgcggccg atgcgatcca cccggcagtg
      661 tttcggcacg gtcggcgcgg aacctcgccg gatcggtgac gaggcgccac gcgcccgcac
      721 cgttggggct gagtgcgtcg cgatcgagaa acttggctgc cgccagccga tcgcgcgcgt
      781 cctcccacgt cgtcggcgca tcggtcttga agtagcgcac acgcttggtc gtgccccaga
      841 tgccctcggg aaggaccacg agcgtgaacg tcgacgaaag ctcgcggttg cgccgactcg
      901 ggcgcgaggc cgttcgtcgg cgcgcgttcc gcatggtcgg gacattggcg gcgctgagcg
      961 cagccatcag cccggtgtag agcgacgcag gcgcgttggg cgttacgatc accgcggcct
     1021 gtccagcggc cttgccccgg cgaaccgcct cggccagatc gacgcgcgcg ccgtcgatcg
     1081 tgattcccgt cgactcgata cgcatgacgc aggtgcgggg tgcgggcgtg ctcggtgcgg
     1141 tcgctggtgt ggtcgagtta ctcgatgcgg tcggggaagc cggcgcgttc gtgtgcttgc
     1201 tcttggtcag gcacccggcc gcggcaccgc ttccgagtcc gagcaaaaat gtagaggtgt
     1261 tcgacatgga cagacctttc agaccagagc ggacggacgc ccgagcgtgg ctcgcgcagg
     1321 tagagcgcga cgacgctctt gcaccacgtc agcttggccg ggttggagag atgcttgctc
     1381 gcgcccgcga gcttggcgtg cgcgctgact tgatccatcg tgatgtcgac ggcgccgagc
     1441 ccttcgaggt agcgcgcgac gcgaccgacg ccgccggcct cggaatagcc ggcgttccag
     1501 ccaaatgtca gtagctcgac aaaccgcgga ttgctccagt tgccttgcag gttcgcgatc
     1561 cgcgggtggt gctttcggta gttgtcgatg atcagccgaa gcagctcgca gccgatcgcg
     1621 acgttgatgc tcggatcgaa gaggtgctcg cgctggtacg gcgtgccgtg gcgcttgttg
     1681 tagtcgtcga gcacaaccgg cacgatctgc atgagcccga gcgctgatcc ggtctgcgtg
     1741 aggtagttgc cgctggattc gcgctcgacg agtgcgcgca cgtattcgat cggcacgctg
     1801 ccccgatagc gctcgaatac cggatcgaac cttcgcacgc gcggcgacgt ggcgaaggat
     1861 gcgctcggtg ttgcgctcgg cgtcggcgtt gccggcgacg ttgcgtgcgg cgcggtcacc
     1921 ccgatgggag gcgtcgttgg gcacggacat gtcgtgccgg tcgccgacgt aggcgcgatc
     1981 ggtgtcgacg tgagcaccag ctgagacggc atcgtgatcg gaccgtcggt cgtcgggacg
     2041 ctcgacgtcg tcggcgtcgt cacgagcgac gtgctcgcag gtctcgtcgg cgcgacggtg
     2101 aggctcggcg ttgcgtccgc atacggatcg atcggccgca tgtctcccga gggtcccgcg
     2161 gcgagcgagc gcatcgagcg gattggcaac gtcgcggcgg gcgcgctgga gctcgacgcg
     2221 ggcagtagcg tcgcggaagc cgtcgtgtcc gactgcttcg cgagacgtgg ttcgacaatc
//
LOCUS       DASUFX010000589         6368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_95406,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000589 DASUFX010000000
VERSION     DASUFX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95406"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFV99_23575"
     CDS             <1..746
                     /locus_tag="VFV99_23575"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5062380.1"
                     /translation="REEIAAAEARVAQTQAVLDKAQLDADRAHRLLQSEAIPKAEADA
                     ADTAVRTAKAARDAQQKILEQVRSGARVEEKAQASAKAAEAQAAANLVLAGPRVEDLR
                     AATAVVEAAQGRLDQLAVTIDELTIRAPAAARVESLDLRPGDLLGPNAPAATVLEEDQ
                     LYVRLYVPETQIGRIKVGQEVPVSVDSFPNRSFKAKVEHINNKGEYSPRNLQTADERA
                     DQVFAVRIGLIEGKDDLRAGMAATVRVPK"
     gene            743..1741
                     /locus_tag="VFV99_23580"
     CDS             743..1741
                     /locus_tag="VFV99_23580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5062381.1"
                     /translation="MTAPDLRPARSSAAIRVDNVSRKFGDFVALSDVDLEVHTGMVYG
                     LLGPNGSGKSTLIRILCGLLAPTTGRASVLGLDVVADSEAIRRQIGYMSQKFALYPDL
                     TVVENLEFYARIYGLRGERLRKRVEGAMALTHIEPYASRRAGLLSGGWKQRLALGAAL
                     MHEPKVVFLDEPTAGIDPVARRELWDLLFDLSAQGITLLVTTHYMDEAERCGEVGYLY
                     LSKLLVTGTPEQLKQMPEVNAPGTRRIEIETPGSTRALGWLRNEPFCRGATVFGQAVH
                     AELDANVSDQDLLARMAQAGFPGATVRPIVPSLEDVFVALTERAARDRGVLQAVSA"
     gene            1741..2844
                     /locus_tag="VFV99_23585"
     CDS             1741..2844
                     /locus_tag="VFV99_23585"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5062382.1"
                     /translation="MWSSFIAIVVKEFKHITRDKGTLALFFTLPVIQLALFGFLDQNV
                     RNLPTVVVDQDQTRFSRELMDEMRATKTFEIVRITNSPDEARALIRQGTVRAGVVIPP
                     DYHDRRARRQQAQFLVLIDGSDSNVSAQALASVNGLVAQHNLDEAEALGAKPLIAAQP
                     IILFNPEGRTANYIIPGLVAILLQLAAMVLASIAIVREREQGTMEQLLVTPIQPIGLV
                     LGKLAPYLVIGLIEMALILLIMRWGFGVPINGSLLLLFAVAVVYLFALLALGLTISMR
                     AQTQMQAVQMAQMLLLPSIFLSGYIFPVAGLPTVLYYIGRALPATHMINVMRGAVLRS
                     AGPFDLLPSLLALAAISIILVVISVRGVRKLTL"
     gene            complement(2841..3389)
                     /locus_tag="VFV99_23590"
     CDS             complement(2841..3389)
                     /locus_tag="VFV99_23590"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5062383.1"
                     /translation="MRQVNVMSDDCDGEMAISIDSLLNEVVESGGEAADGSDVPEVSR
                     KVTPRSRGKRDRPRSKTIALKRLTRAELLRGAVENPPVDDVERPHTREDCRDAPRPCP
                     WVACKHHLYLDINPRTGSIKINFPDLEPWELAHTCALDVADNGALTLEEIGLITNLTR
                     ERVRQVEVRGLLTLRARSHSLR"
     gene            3635..4222
                     /locus_tag="VFV99_23595"
     CDS             3635..4222
                     /locus_tag="VFV99_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062384.1"
                     /translation="MNRLPMRWGGARRGAGRPARGPIASEPHKRRPQLSPRHPVHVTA
                     RFAHTDRACSAETTQLARGATYRVLRRALDLSLARTDFRIVHLAVVASRLELIVEADD
                     KHALARGMQGFQVSAAKWLNRAARRRGTVFPDRYRMRILATRVDVRDAIGRLPDAPSA
                     RTAAWPETWLLSAELAVTSERSQRRWIRSRADEDS"
     gene            complement(4164..4571)
                     /locus_tag="VFV99_23600"
     CDS             complement(4164..4571)
                     /locus_tag="VFV99_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062385.1"
                     /translation="MYGALLGLGNTAVIALGIAVREHGGAPAFMALLFIGSFVGVPSG
                     MVIGAIAKRTPGWPVFARLVVIIAPAFFVVTLFGTVARFDAYIVLSFVPTIVAALYLE
                     RWTRATQPLPAAQRITNLHQRETESNAVANARW"
     gene            complement(4841..5518)
                     /locus_tag="VFV99_23605"
     CDS             complement(4841..5518)
                     /locus_tag="VFV99_23605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5062386.1"
                     /translation="MTDHRVLSHLGVAAREYDVAIRRYIPRYEEMIATAVSLARGDVI
                     DLGSGTGALAAAILEAQPSARVKLVDIDPAMLETARERVAHHGDRAELVHASFDTLWS
                     NAPRCDTVVASLALHHVPEIEKKRTLYARIYEALRPGGAVIVADCNVHEHGRERDQMF
                     ATWSAWMAQHGIAQPEANDLFAKWAGEDRYYPIAVELHLLADAGFARPDVFWKYGPIS
                     VYGAFRD"
     gene            5624..>6368
                     /locus_tag="VFV99_23610"
     CDS             5624..>6368
                     /locus_tag="VFV99_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062387.1"
                     /translation="MSKSPLATLFACTAAIYVTATHLPLLHADESANAQFCKETGFYT
                     SSEFSPKDFANIQMTEDGATETAGRFGNMMCTGGKVTGRDKIMAIRASWMKSLGIDER
                     DFVVLAALSNARTYENQNLSSIAGPVSQIGNGYAAAGLVELDALGANASMFARFTVVD
                     RCLQSGAERSPLLRHILCTREQLDPTKAYAELDATAELNNGTRYRLRRLVRKTAEAAA
                     KRRAELDALAKTDPGVAKVIAIADAQFKEW"
BASE COUNT         1099 a         2178 c         2036 g         1055 t
ORIGIN      
        1 ggcgtgaaga gattgcagcc gccgaagcac gcgtcgcgca gacgcaggcg gtgctcgaca
       61 aagcgcaact cgatgccgat cgcgcgcatc gcttgctgca gagcgaagcg attccgaagg
      121 cggaagccga cgcggctgac accgcggtcc gcaccgcgaa ggccgcgcgc gacgcgcagc
      181 agaagatcct cgagcaggtg cgctcgggcg cgcgcgtcga agagaaggcg caggcatcgg
      241 cgaaggccgc agaagcgcag gccgcggcga acctcgtgct cgccggcccg cgggtcgagg
      301 atctgcgcgc ggcgaccgcg gtggtcgaag ccgcgcaagg ccgcctcgat cagctcgcag
      361 ttacgatcga cgagctgacg attcgcgcgc ccgcagccgc gcgcgtcgag tcgctcgacc
      421 tgcggcccgg tgacctgctt ggccccaacg cgccggcagc gacggtgctc gaagaggatc
      481 agctctacgt gcggctctac gtgcccgaga cccagatcgg gcgcatcaag gtcgggcaag
      541 aagtcccggt ttcggtcgac tcgttcccga atcgttcgtt caaggcgaag gtcgagcaca
      601 tcaacaacaa gggcgagtac tcgccgcgca acctccagac tgcggacgag cgcgccgatc
      661 aggtgttcgc ggttcgcatc ggcctcatcg aaggcaaaga cgatctgcgc gccggtatgg
      721 cggcgacggt acgggtgccg aagtgaccgc gcctgaccta cgcccagccc gctcgtcagc
      781 ggcgatacgc gtcgacaacg tatcgcgcaa gttcggtgat ttcgtcgcgc tgtccgacgt
      841 cgacctcgag gtgcacacgg ggatggtcta cgggttgctc ggcccgaacg gctccggcaa
      901 atcgacactg atacggatcc tgtgcggact gctcgcgcca accaccggtc gcgcatcggt
      961 gctcgggcta gacgtcgtcg ccgacagcga agcaattcgc aggcagatcg gttacatgag
     1021 ccagaagttc gcgctctacc ccgatctcac ggtcgtcgag aacctggagt tctacgcgcg
     1081 catctatggg ttgcgtggcg agcgactgcg caagcgtgtc gagggcgcaa tggcgctgac
     1141 tcacatcgag ccgtacgcga gccgccgcgc ggggctactc tccggtggct ggaagcagcg
     1201 gctcgcactc ggggccgcgt tgatgcacga accaaaggtc gtgtttctcg acgagccgac
     1261 cgccggcatc gatcccgtcg cacgccgcga gctgtgggac ctgctgttcg acctgtcggc
     1321 acagggcatc acgctgctcg tcaccacgca ctacatggac gaggccgagc gctgcggtga
     1381 ggtcggctat ctgtatttgt cgaagctcct ggtcacgggc acaccggaac aactcaaaca
     1441 gatgccggaa gtgaacgctc cgggcacgcg tcgcatcgag atcgagacgc cgggttcgac
     1501 acgtgctttg ggctggctcc gcaacgaacc gttctgccgc ggcgccaccg tgttcggtca
     1561 ggctgtccac gccgagctcg atgccaacgt gtctgaccag gacctgctcg cgcggatggc
     1621 ccaggccggc tttcccggcg cgacggtgcg accgatcgtg ccgtcgctcg aagacgtctt
     1681 cgttgcgctc accgaacgtg ccgcgcggga tcgcggcgtc ctccaggcgg tgagtgcctg
     1741 atgtggtcgt cgttcatcgc gatcgtggtc aaggagttca agcacatcac gcgggacaag
     1801 ggcacgctcg cgttgttctt cacgctgcca gtgatccagc tcgcgctgtt cggcttcctc
     1861 gatcagaacg tgcgcaacct gccgaccgtg gtcgtcgatc aggaccagac ccggttctcg
     1921 cgcgagctca tggacgagat gcgcgcgacc aagacgttcg agatcgtgcg catcacgaac
     1981 agccctgacg aggctcgcgc gctgatccgt caaggtaccg tgcgtgcggg cgtcgtgatt
     2041 ccacccgatt accacgaccg ccgcgcacgg cggcagcagg cgcagttcct cgtcctcatc
     2101 gatggctccg actcgaacgt cagcgcccag gcgctcgcgt cggtcaacgg gctcgtcgcg
     2161 cagcacaacc tcgacgaagc cgaagcactc ggagccaagc cgctgatcgc ggcgcagccg
     2221 atcatcctgt tcaaccccga gggccggacc gcgaactaca tcatccccgg cctcgtcgcg
     2281 atcttgctcc agctcgcggc catggtgctt gcctcgatcg cgatcgttcg cgagcgcgag
     2341 caaggcacga tggagcagct gctcgtcacg ccgatccagc ccatcgggct cgtgctcggt
     2401 aagctcgctc cctatctggt gatcggtctg atcgagatgg cgctgatcct gctcatcatg
     2461 cgctggggct tcggcgtgcc aatcaacggc agtctgttgc tcttgttcgc ggtcgcagtt
     2521 gtctacctgt tcgcgctcct cgcgctgggg ctcacgatct cgatgcgcgc tcagacccaa
     2581 atgcaggcgg tccagatggc gcagatgctg ctgctgccgt cgatcttcct gagcggctat
     2641 atcttcccgg tcgccgggct gccgacggtc ctctattaca tcggccgcgc gcttcccgcg
     2701 acgcacatga tcaacgtgat gcgtggcgcc gtgttgcgct cggcggggcc gttcgatcta
     2761 ttaccgagcc tgctcgcgct cgccgcgatc agcatcatcc tcgtagtcat ctcggtacgc
     2821 ggcgttcgca agctgacgct ctaacgcagc gagtgcgacc gcgcgcgcag cgtcaacagg
     2881 ccgcgcacct cgacctggcg cacacgttcg cgcgtcaggt tcgtgatcag cccgatctcc
     2941 tcgagcgtga gcgcgccgtt gtcagcgaca tcgagcgcgc acgtatgcgc gagctcccac
     3001 ggctcgagat ccggaaagtt gatcttgatc gagccggtcc gcggattgat gtcgagatac
     3061 agatgatgct tgcacgcgac ccacggacac gggcgcggcg cgtcgcgaca gtcctcgcgc
     3121 gtgtgtggcc gctcgacatc gtcgaccggc ggattctcca ccgcgcctcg cagcagctcc
     3181 gcgcgcgtca atcgcttgag cgcgatcgtc ttgctgcgcg gccggtcccg cttgccgcgc
     3241 gaccgcggcg tgaccttgcg acttacttca ggaacgtccg aaccgtccgc cgcctctcct
     3301 ccgctctcga caacttcatt cagtaacgaa tcgatcgaga tcgccatctc cccatcgcag
     3361 tcgtcgctca tgacattgac ctgcctcatg cgggcgtgac tggaagctga cgacgcgcga
     3421 gcgggctcag caccagagcc ccatggtgct cgcatcgcgt ctggtcagct caccaaggtt
     3481 cccgcgttag tcaccctaat ggcgcttaca taagctacgc aagaacattt gctcaaaatt
     3541 tgcctggaga gatctcggcg atttcatatt tctggtgtac ggatcgcatg ccgatgaacg
     3601 ggccgcacca aatcgagccg cgaaacggcg cgcgatgaac cgcttgccga tgcgctgggg
     3661 cggcgctcga cgtggcgccg gccgtcccgc acgcggcccg atcgcgagcg agccgcacaa
     3721 acgacgcccg cagctgtcgc cgcgccatcc ggttcacgtc accgcgcgct tcgcacacac
     3781 tgatcgcgcg tgcagcgcgg agactacgca gctcgcgcgc ggcgctacct atcgcgtcct
     3841 acgccgcgcg ctcgacctct cgctcgcgcg caccgacttc cgcatcgttc acctcgcggt
     3901 ggtcgcgtcg cggctcgagt tgatcgtcga agccgacgac aagcatgcgc tcgcgcgcgg
     3961 catgcagggc tttcaggtct cggcggccaa gtggctcaac cgcgccgctc gccgccgcgg
     4021 caccgtcttc cccgaccgct accggatgcg gatcctcgcc acgcgcgtcg acgtgcgtga
     4081 cgcgatcggc cggctccccg atgcaccaag cgcgcgcacc gcggcctggc ccgagacctg
     4141 gctgctcagc gccgagctcg ccgttaccag cgagcgttcg caacggcgtt ggattcggtc
     4201 tcgcgctgat gaagattcgt gatccgctgc gcggcgggta gtggctgcgt ggcacgggtc
     4261 cagcgttcga gatagagcgc cgccacgatc gtgggcacga acgacagcac gatgtacgcg
     4321 tcgaagcgcg ccaccgtgcc gaatagcgtc acgacgaaga atgccggcgc gatgatcacc
     4381 acgagccgcg cgaacaccgg ccaaccgggc gttcgcttcg cgatcgcgcc gatcaccatt
     4441 ccggacggca caccgacgaa cgagccgatg aacagcaacg ccatgaatgc gggcgcacca
     4501 ccgtgctctc ggacggcgat gccaagcgcg attaccgctg tgttgcccag gccgagcaag
     4561 gctccgtaca tgcgggcggt catctgcgaa taacggacca ggggcccgcg ttattgcagc
     4621 ccaaccggtt tcgcgcagct agcgcgggcc gggtgtcatc ccgtcgacaa ccgccgcctt
     4681 ggcgcctgcc atttcgtcat gcgagccagt cctgccttcg aaccaacatc gagtgcccgc
     4741 gatgcgtgtc gccacagcgg ctgtccggaa tccgggcgaa aagttagtca ccgatccgcg
     4801 agcgacgctt gctcgtcatt gcgacgtgtg cgctaacgac tcagtcgcgg aacgcgccat
     4861 acacgctgat cgggccgtac ttccagaaca cgtccggccg tgcgaagccg gcgtcggcga
     4921 gcagatgcag ctcgaccgcg atcgggtagt agcgatcttc gcctgcccac ttcgcgaaca
     4981 ggtcgttggc ctcgggctgc gcgatgccgt gctgcgccat ccacgctgac caggtcgcga
     5041 acatttggtc ccgctcgcgg ccgtgctcgt gcacgttgca gtccgcgacg atcactgcgc
     5101 cgccgggacg cagcgcctcg tagatccgcg cgtagagcgt gcgcttcttt tcgatctcgg
     5161 gcacgtgatg gagcgcgagc gacgcgacga ccgtgtcgca acgcggcgcg ttcgaccaga
     5221 gtgtgtcgaa cgaggcgtgc acgagctcgg cacgatcgcc gtggtgagcg acacgctcgc
     5281 gcgcggtctc gagcatcgct ggatcgatgt cgacgagctt cacacgcgcg ctcggttgcg
     5341 cttcgaggat cgcagccgcg agcgcaccgg tgccggagcc gagatcgatc acgtcgccgc
     5401 gcgcgagcga gaccgccgtc gcgatcatct cctcgtagcg agggatgtag cggcggattg
     5461 cgacgtcgta ttcacgagcc gccacgccga ggtggctgag cacgcggtgg tcggtcatcg
     5521 gcgcgatcta gcacgggccg ggcggcttgg gtcggaaccc agcgggatca cgggactgcg
     5581 agacaggccg caaccagcgt ccgcacctgt cgatacgcgc gccatgtcca aatcaccact
     5641 cgcgacactt ttcgcatgca ccgccgccat ctacgtgacg gccacacacc ttccccttct
     5701 acacgccgac gaatcggcca acgcccagtt ctgcaaggag accggcttct acacgagctc
     5761 cgagttctct ccgaaggact tcgcgaacat ccagatgacc gaggacggcg cgaccgaaac
     5821 ggccggccgc ttcggcaaca tgatgtgtac aggtggcaag gtcaccggcc gcgacaagat
     5881 catggccatc cgcgcgagct ggatgaagtc gctcggcatc gacgagcgcg actttgtcgt
     5941 gctagccgcg ctcagcaacg cgcgcacgta cgaaaatcag aacctgagct cgatcgccgg
     6001 cccggtgagc caaatcggga atggctacgc cgcggcgggg ctcgtcgagc tcgacgcgct
     6061 gggcgcgaac gcatcgatgt tcgcacgctt cactgtcgtc gatcgctgtc tacagtcggg
     6121 agccgaacgc agcccgctcc tgcgccacat cctgtgcacc cgcgagcagc tcgatccgac
     6181 gaaagcgtac gcggagctcg atgccaccgc cgagctcaac aacggcaccc gctacaggct
     6241 gcgccgcctg gtgaggaaga ccgccgaggc cgctgcgaag cgccgcgccg agctcgacgc
     6301 actcgcgaag accgatcctg gcgtcgccaa ggtgatcgcg atcgctgacg cgcagttcaa
     6361 ggagtggg
//
LOCUS       DASUFX010000590         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_235993,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000590 DASUFX010000000
VERSION     DASUFX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235993"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFV99_23615"
     CDS             <1..1194
                     /locus_tag="VFV99_23615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5062388.1"
                     /translation="ARIRVADCALALGDPRAANVYRAVAWYCLKSGHPLAALVCARVL
                     EAHGADSSDILASLVVTYGNESERLGKVAARIALPADTINLEVPDVRLAAPPDAVATA
                     IARAEHATDNFKEYPESLHAIPLLSTMSEAAFKRVLSTLTLRRMPVGAPVIREGEAGN
                     SFFFVAGGGLRVYATDGLGRQTELAKLGENAVFGEMALLSAQPRTATVECTSDVDLFE
                     ASRQSLAALADELGAVAEALHGFTRERLLGNLMATSALFKPFNRMQQRDLLRRFTSHD
                     VAPGTVVINEGEQGRGLFVVLSGELDVSRRAADGTTVPLGGLKTGDVFGEMSILRNAR
                     TTATVVATRPATVLFLAGEYVTRIVSGVPEIKSFLEALAEGREIDNQLLLGEDETPAD
                     ERILI"
     gene            1212..3071
                     /locus_tag="VFV99_23620"
     CDS             1212..3071
                     /locus_tag="VFV99_23620"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5062389.1"
                     /translation="MDDEQQTSERPGPSKILWLLLALAIVLMWWYFRPRHDEGAGAAQ
                     EMSDQASAATDPDDILVDLKDNATPQQVAAIERDLGIDLVLVSDQSKDEQFYRAHVDP
                     ARRDAIIDALSKRSEVEIAEPDATVSLSPGEETVAPIVPTWEGFPNDPQYKFQWHMRQ
                     IGMPEAWKLADGNGVIVAVLDTGVGYEDYANFHVLPDLKGITFVKPYNFVANNKHAGD
                     DHGHGSHVTGTIAQVTNNGIGVTGVAMNVKIMPLKVLSAGGSGSVAGIADAIRYAADE
                     GAKVINMSLGGAFPSRVLKKAVEYAHAKGVTVVCAAGNESRGKVGYPAAYPGAIAVSA
                     TQYDENTTFYSNYGKDIDVAAPGGNTRVDQNGDGMPDGVLQNTIVIGDPTKDDYFGYM
                     GTSMASPHVAGVAALVVGEGVSDPDMVEKILKDTARKPKDSKQYTTDKYGAGIIDAPA
                     AVLKARAEGGGWQLGLGLLMAGAVAASARRKGLGVKLGPSYLAGVVLGASGLFFLPYI
                     FPSLSSAPVVDTLTRGLPSWDLSLLGPSGHGNVLFFSALVPLALLAVGYGVPKLRAPL
                     AGLAIGVAAHLAFFAVVPMTQLHYVPLGFGLASIWLGLNAIACVLLARLALRR"
     gene            3265..>3586
                     /locus_tag="VFV99_23625"
     CDS             3265..>3586
                     /locus_tag="VFV99_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062390.1"
                     /translation="MKTSHSIIAALALLNTANAFAQPAMDAPAAEVDTAVTVEPTTAT
                     ESAPVDQVKPRKRHHVRMALELGAIFAAGTRWYWRDGGEPNRVDWQLGFGGDALKKKL
                     VGFGD"
BASE COUNT          595 a         1214 c         1157 g          620 t
ORIGIN      
        1 gcacgaattc gcgtcgcaga ttgtgcgctc gcgctcggcg atccacgtgc cgccaacgtg
       61 taccgcgccg tcgcttggta ctgcctgaag tccggacatc cactcgcggc tctcgtgtgc
      121 gcgcgcgtgc tcgaagcaca cggcgcggat tcttccgaca tcctcgcgtc gctcgtcgtc
      181 acctacggca acgagagcga gcgcctcggg aaggtcgcgg cacgcatcgc gctccctgcc
      241 gacacgatca atctcgaagt gcccgacgtc cgcctcgccg cgcctcccga cgctgttgcc
      301 actgcgatcg cacgcgcgga gcacgcgacc gacaacttca aggagtaccc cgaatcgctc
      361 cacgcgattc cgctgctctc gacgatgtcg gaggccgcgt tcaagcgcgt gctgtccacg
      421 ctcacgttgc gccgcatgcc ggtcggtgcg ccggtgatcc gcgaaggcga agcgggtaac
      481 tcgttcttct tcgtcgccgg cggtgggctc cgcgtctacg cgaccgatgg tctcggccgc
      541 caaaccgagc tcgcgaagct cggcgagaac gctgtgttcg gcgagatggc attgctctcg
      601 gcgcagccgc gaacggccac cgtcgaatgc acctccgacg tcgacctctt cgaagcgtcg
      661 cgccaatcgc tcgccgcgct cgccgacgag ctcggtgcgg tcgccgaagc acttcacggc
      721 ttcacgcgag agcgcctgct cggcaacctg atggcgacga gcgcgctgtt caagccgttc
      781 aatcggatgc agcagcgcga cctgctccgt cgcttcacca gccacgacgt cgctcccggc
      841 acggtcgtca taaacgaagg cgaacaaggc cgcggtctgt tcgtcgtgct atcgggcgag
      901 ctcgatgtct cgcgccgcgc cgcggatggc accacggttc cactcggcgg cctcaagacc
      961 ggtgacgtgt tcggcgagat gtcgatcctg cgaaacgctc gcaccaccgc taccgtcgtt
     1021 gcgacgcgtc ctgcgacagt tttgttcctg gcgggcgaat acgtcacgcg tatcgtttcc
     1081 ggcgtgcctg aaatcaaaag ctttctcgaa gcgttggccg aggggcgcga aatcgacaat
     1141 cagcttttgc tcggtgaaga cgaaactccg gcggacgagc gaattctgat ttagggccta
     1201 tacttgtcgt catggacgac gagcagcaga cctctgagcg cccgggaccg agcaagatcc
     1261 tgtggctcct cctcgccctc gcgatcgtgc tcatgtggtg gtacttccgc ccgcggcatg
     1321 acgagggcgc tggtgccgca caggagatgt cggatcaggc gagtgccgct accgaccccg
     1381 acgacatcct cgtcgatcta aaggacaacg cgacgccaca gcaggtcgcc gcgatcgagc
     1441 gcgacctcgg catcgatctc gtgctggtca gcgaccagtc gaaggacgag cagttctatc
     1501 gcgcgcacgt cgatccggcg cgccgcgacg cgatcatcga tgcgctctcg aagcgctccg
     1561 aggtcgagat tgccgagccc gacgcaaccg tctcgctctc gcccggcgaa gagaccgtcg
     1621 cgccgatcgt cccgacgtgg gaaggcttcc cgaacgaccc gcaatacaag tttcagtggc
     1681 acatgcgcca gatcggcatg cccgaggcgt ggaagctcgc cgacggtaac ggcgtgatcg
     1741 tcgcggtgct cgacaccggc gtcggctacg aggactacgc caacttccac gtgctgcccg
     1801 atctcaaggg catcacgttc gtgaagccgt acaacttcgt cgccaacaac aagcacgccg
     1861 gcgacgatca cggccacgga tcgcacgtga ccggcacgat cgcgcaggtc acgaacaacg
     1921 gcatcggtgt caccggtgtc gcgatgaacg tcaagatcat gccgctcaag gtcctctcgg
     1981 ccggtggctc gggctcggtc gcaggcatcg cggacgcgat tcgctacgcg gccgacgaag
     2041 gcgcaaaggt catcaacatg tcgctcggcg gtgcgttccc gtcgcgcgtg ctgaagaagg
     2101 ccgtcgagta cgcgcacgcg aagggcgtca ccgtcgtgtg cgccgcgggc aacgagagcc
     2161 gcggcaaggt cggttatccg gccgcgtatc cgggcgcgat cgccgtctcg gcgacgcagt
     2221 acgacgagaa caccacgttc tactcgaact acggcaagga catcgacgtc gctgcgcctg
     2281 gcggtaacac gcgcgtcgat cagaacggcg acggcatgcc cgacggcgtt ctccagaaca
     2341 cgatcgtgat cggtgatccg acgaaggacg attacttcgg ttacatgggc acgtcgatgg
     2401 cgtcgccgca cgtcgccggt gttgccgcgc tcgtcgtcgg cgaaggcgtc agcgatccgg
     2461 acatggtcga gaagatcctc aaggacaccg cgcgcaagcc gaaggacagc aagcagtaca
     2521 cgaccgacaa gtacggcgcg ggcatcatcg atgcgccggc cgcagtgctc aaggcgcgtg
     2581 cggaaggcgg cggctggcag ctcggcctcg gcctgctcat ggcaggcgcc gtcgctgcga
     2641 gcgcgcgtcg caaaggtctt ggcgtgaagc tcggcccgag ctacctcgcg ggcgtcgtgc
     2701 tcggcgcgtc gggtctgttc ttcctgccgt acatcttccc gtcgctgtcg tcggcgccgg
     2761 tcgtcgacac gctcacgcgc ggtctgccgt cgtgggatct ctcgctgctc ggcccgagcg
     2821 gtcacggcaa cgtgctgttc ttcagcgcgc tcgtgccgct cgcgttgctc gcagttggtt
     2881 acggcgtgcc gaagctacgc gcgccgctcg ctggtctcgc gatcggcgtt gctgcgcacc
     2941 tcgcgttctt cgcggtggtg ccgatgaccc agctgcatta cgtgccgctc ggcttcggtc
     3001 tcgcctcgat ctggctcggt ttgaacgcga tcgcgtgcgt gttgctcgcg cggttggcgc
     3061 ttcgacgcta ggttgtcaga cgttcgacag gtcgcgtttt ctcgtcggag atcacgcgat
     3121 cttcacgttt gacacccgcg gtaggtaacg cgggaggcca tgcgcgcgtc acgcgctagg
     3181 ccggcgctcg tcacgcaatc gctcacgtct tgcccacgtt cgcgctcagg cgggttcacg
     3241 cggtgaaacc gcagtgtgaa gtccatgaag acttcgcact cgatcatcgc agctctcgcc
     3301 ctcctcaaca ccgccaacgc gttcgcgcag cccgcgatgg acgcgccggc cgccgaggtc
     3361 gacaccgccg tgacggtcga gccgacgacg gcgaccgagt cggcaccggt cgaccaggtc
     3421 aagccgcgca agcgccacca cgtgcgcatg gcactcgagc tcggcgcgat cttcgcggcc
     3481 ggcacgcgct ggtactggcg cgatggtggc gagccgaacc gcgtcgattg gcagctcggc
     3541 ttcggcggtg acgcgctgaa gaaaaagctg gtcggctttg gcgacg
//
LOCUS       DASUFX010000591         5595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_95566,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000591 DASUFX010000000
VERSION     DASUFX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95566"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFV99_23630"
     CDS             <1..232
                     /locus_tag="VFV99_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062391.1"
                     /translation="EIDVGSAKKNAAVQHDLGINVNAIPLAVFYEPTGELVGDTFAGE
                     LKPSRHFSSREIRDFLREVVDYHRIVSPDQRQ"
     gene            296..811
                     /locus_tag="VFV99_23635"
     CDS             296..811
                     /locus_tag="VFV99_23635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5062392.1"
                     /translation="MLREDDAAELFVLTDANRAYLRRWLPWVDLVTSEEDSRSFLATV
                     AAQREEGRGPTFGVLCDGALAGVIGFLPVDRVNRIGEIGYWLAPQSQGRGVMTQSCRF
                     VVRYGFLTLDLNRIQIAAGTENRASRAIAERLGFRFEGILRARENLYGTFIDHAMYSL
                     LRSEFDEAELQ"
     gene            856..1659
                     /locus_tag="VFV99_23640"
     CDS             856..1659
                     /locus_tag="VFV99_23640"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX5062393.1"
                     /translation="MSESTASRRYLKWLAAGAVAVAAVGIAAWAQLGVHRAPAVAGFK
                     VVAAYPHDPAAFTQGLAIEAGKLYEGTGLYGASTVRRVNLMSGRAEKQRALGERYFGE
                     GIAILGGRLYQITWMNGAAVVYNVDTFEVERTMQYDGEGWGLTHDGKELIMSDGSATI
                     RFRDPQTFAVTREIEVRDNGMPLAMLNELEYIDGEIWANIWYDDRIARISPADGRVLG
                     FIDLSTLYPKSARGSEAVLNGIAYDAAAKRLFVTGKNWPQLYEIEVTRP"
     gene            1715..3304
                     /locus_tag="VFV99_23645"
     CDS             1715..3304
                     /locus_tag="VFV99_23645"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5062394.1"
                     /translation="MAKRSLRSIFVHKSVEQMHAEHERGELKKSLGAVNLVLLGIGCI
                     IGTGIFVLTGQAAALHAGPAIMISFIITGTLCTLVALAYAELASAIPVSGSAYSYSYA
                     SLGEFAAWIMGVLLVLEYGAAASTVAVGWSGYVASFLHDIGIDIPVALRGAIGTPITD
                     FASGAVVGTGVVNLPAVIGCAAVTALLVLGVSESAFVNNIIVAIKVTVVVAFIIIGSF
                     FVNTDLWFPLVPQAVPPHPQGTSLWSEIGTALWDVVTAQASGRYGIAGIITAAATIFF
                     AYIGFEAVSTAGAESRNPKRDMPIGILGSLIICTILYILTSAVLVGIVPYTDLNDPAP
                     IAKAVNQIGLPWFAALVKIGAIAGLSSVMLVLLYGQTRIFYTMARDGLIPPLFSAVHS
                     KFKTPWINTLIVGLLACAFAGFMGLDTLAKVTNVGTLAAFVIVCITVIYLRYKRPDMP
                     RQFRTPWFPVTPVLGAVMSAFLLMSLMSDAATRNFFIPYLLIGVVLYFAYGMWNSNLG
                     KGIVVHGHEVMADSPHPKGLD"
     gene            3323..3961
                     /locus_tag="VFV99_23650"
     CDS             3323..3961
                     /locus_tag="VFV99_23650"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5062395.1"
                     /translation="MSAFDMFLHVDQHLGEFATSYGVWIYALLFLVIFLETGVVVTPF
                     LPGDSLLFAVGALAAAGVIDIVGILSLLTAAAIIGDNTNYFIGRAVGPRVFTEHSKWL
                     KREHLMRTQRFYEVHGGKTVIMARFVPIVRTFAPFVAGVGRMRYPRFLAFDVAGGILW
                     VWGFGLLGYFTGAIPFVKENFGLVTIGVILISVVPLAFEILRARLQRARAGV"
     gene            3972..5228
                     /locus_tag="VFV99_23655"
     CDS             3972..5228
                     /locus_tag="VFV99_23655"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5062396.1"
                     /translation="MTLGHVTLIALAVAVVVSCVSRLNVGLLAMALAWIIGVFIGGLR
                     LAEVLAGFPVDLFITLSGVTLLFAQAHVNGTLDIVAHNAMRLCRGRVGLVPIMYFALG
                     AALASAGPGNIATTGLLAPMAMATALKNRTSPFLMAIMVGNGCNAGSLSPLAPTGLIV
                     TDYMSRIGLPNFELQTWAYNLMAHAIVAFAGYFAFGGYKLLMRADRVEVEAEPRKQLD
                     GHQILTLAVIAVLIVTAIASQLFDIRGFNIGLAAIAGAVLLALARAADDGQAIKTVPW
                     ATILMVCGVTVLIGLLQKTGGMEIFSNLLSSIATPATVIPELSVIIGIVSAYSSTSGV
                     VLPAFLPTVPGLVENLNLDNALGIASTMNIAGHLVDVSPLSTIGALCIASVPAGEMSR
                     KLFNQLLAWGMSMTVVGAVLCWVLFA"
     gene            complement(5242..>5595)
                     /locus_tag="VFV99_23660"
     CDS             complement(5242..>5595)
                     /locus_tag="VFV99_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062397.1"
                     /translation="AEGHDWPAIFAAFEADRAPNAHAIAEMALENYREMRDDVRDPKF
                     QLRADLSFELERRFPGRFIPRYSMVMFHPEISYAEAQRRGVVQAQILRELSDAVDSLP
                     QVDFARARTLVARSL"
BASE COUNT          864 a         1813 c         1800 g         1118 t
ORIGIN      
        1 cgagatcgac gtcggcagcg cgaagaagaa cgccgccgtg cagcacgatc tcggcatcaa
       61 cgtgaacgcc attccgctcg ccgtgttcta cgagccgacg ggcgagctcg tcggcgacac
      121 gttcgcgggc gagctgaagc cttcgcgcca cttttcttcg cgcgagattc gtgacttcct
      181 ccgcgaagtc gtggactatc accgcatcgt cagccccgac cagcggcagt gagagtcgcg
      241 cggcggtaag ccgcgctcag caatgttcgt catcgattca gcggtatcct tgcgcgtgct
      301 gcgcgaggac gatgccgcgg agctcttcgt gctcacggac gccaatcgcg cgtatctgcg
      361 ccgttggctg ccgtgggtcg atctcgtgac gtcggaagag gactcgcggt cgttcttggc
      421 gactgtagca gcgcaacgcg aggaaggccg cggccccacg ttcggcgtgc tttgcgacgg
      481 ggcgctcgcc ggcgtcatcg gcttcttgcc cgtcgaccgc gtcaatcgaa tcggcgagat
      541 cggctactgg ctcgcgccgc agtcgcaggg ccgcggcgtg atgacgcaat cgtgccggtt
      601 tgtcgtgcgc tacgggttcc tcacgctgga cttgaaccgc atccaaatcg cggccggcac
      661 ggagaatcgg gcgagccgtg ccatcgcgga gcggctcggc ttcaggttcg aaggaatcct
      721 gcgcgcccgc gagaatctct acggcacgtt catcgaccac gcgatgtact cgctgctgcg
      781 cagcgagttc gacgaggccg agcttcagtg aaccgcgctt gccggccctt ggcgggtttg
      841 ccaaagtagc ccgctatgtc cgaatccaca gcgtctcgac ggtatctcaa gtggctcgcg
      901 gccggcgcgg tcgcggtcgc cgccgtcggc atcgcggcat gggcgcagct cggcgtgcac
      961 cgcgctcccg cagtcgcagg cttcaaggtc gtcgcggcgt acccgcacga tcccgcggcg
     1021 ttcacgcagg gtctcgcgat tgaagctgga aagctttacg aaggcaccgg cctctacggc
     1081 gcgtcgaccg tgcggcgcgt gaatctcatg agcggccgcg ccgagaagca gcgcgcgctt
     1141 ggcgagcgct acttcggcga aggcatcgcg atcttgggcg ggcgcctcta tcagatcacg
     1201 tggatgaacg gtgccgccgt cgtctataac gtcgacacgt tcgaagtcga gcgcacgatg
     1261 cagtacgacg gcgaaggctg gggcctcacg cacgacggta aagagctcat catgagcgac
     1321 ggctccgcga cgatccgctt ccgcgatccg cagacgttcg cggtcacgcg cgagatcgag
     1381 gtgcgcgaca acggcatgcc gctcgcgatg ttgaacgagc tcgagtacat cgacggcgag
     1441 atctgggcga acatctggta cgacgaccgc atcgcgcgca tttcgccggc cgacggccgg
     1501 gtgctcggct tcatcgactt gagcacgctc tatccaaaaa gcgcgcgcgg cagcgaggcc
     1561 gtgctgaacg gcatcgccta cgacgctgct gcaaagcgcc tgttcgtcac gggcaagaat
     1621 tggccgcagc tttacgagat cgaggtcacg cggccgtgac ccttgcgcac ggcctggtgt
     1681 agagtccacc ccaggtgtaa tcgggagctc cttgatggcc aagcgttctc tacgcagcat
     1741 cttcgtgcac aagtccgtcg agcaaatgca tgcggaacat gagcgcgggg agctgaagaa
     1801 gtcgctcggc gccgtcaatc tcgtactact cggcatcggt tgcatcatcg gcaccggcat
     1861 cttcgtcttg acggggcaag cggccgcctt acacgcgggc cccgcgatca tgatctcgtt
     1921 catcatcacg ggtacgttgt gcacgctcgt cgcgctggcc tacgcggagc tcgcgtcggc
     1981 gatcccagtg tcgggctcgg catactcgta ctcgtacgcg tcgctcggcg agttcgccgc
     2041 ctggatcatg ggcgtgctgc tcgtgctcga gtacggcgcc gcggcctcga cggtcgccgt
     2101 gggctggtcc ggctacgtcg cgagcttcct gcacgacatc ggcatcgata tccccgttgc
     2161 tcttcgcgga gcgatcggca cgccgatcac ggacttcgct tcgggcgcag tcgtcggcac
     2221 gggcgtcgtg aacctgcccg cggtcatcgg ctgcgccgcc gtcacggctc tgttggtgct
     2281 cggcgtgtcg gaatcggcgt tcgtcaacaa catcatcgtg gcgatcaagg tcacggtcgt
     2341 cgtcgcgttc atcatcatcg gctcgttctt cgtgaacacc gatctctggt ttccgctcgt
     2401 gccgcaggcc gtgccgccgc acccgcaggg cacgagcctg tggagcgaga tcggcacggc
     2461 gctgtgggat gtcgtgacgg cgcaggcctc ggggcgatac ggcattgccg gaatcatcac
     2521 ggccgccgcg acgatcttct tcgcgtacat cggcttcgag gccgtgtcga cggccggcgc
     2581 ggagtcgagg aacccgaagc gcgacatgcc gatcggcatt ttgggctcgc tgatcatttg
     2641 cacgatcctc tacattctga cgtcggccgt gctcgtcggc atcgttccgt acacggatct
     2701 caacgacccc gcgccgatcg cgaaggccgt caaccagatc ggcctgccgt ggttcgccgc
     2761 gctcgtgaag atcggcgcga ttgccggctt gtcgtcggtg atgctcgtcc tgctatacgg
     2821 gcagacgcgc atcttctaca cgatggcgcg cgacggattg atcccgccgc tgttctccgc
     2881 cgtccactcg aagttcaaga cgccgtggat caacacgttg atcgtcggcc tactcgcgtg
     2941 cgcgtttgcg ggcttcatgg gtctcgacac gctcgcaaaa gtcaccaacg tcggcacgtt
     3001 ggccgccttc gtgatcgtct gcatcaccgt catctatctg cgctacaagc gtcccgacat
     3061 gccgcggcag ttccggacgc cttggttccc ggtgacgccg gtgctgggtg ccgtcatgag
     3121 tgcctttctc ttgatgtccc tgatgagcga cgcggcgacg aggaatttct tcattcctta
     3181 tctgctcatc ggcgtcgtcc tgtacttcgc gtacggcatg tggaactcca acctcggcaa
     3241 gggcatcgtc gtgcacggcc acgaagtcat ggccgactcg cctcacccga agggtctgga
     3301 ctgatcgcga atggattttt tgatgagcgc gttcgacatg tttctacatg tcgaccaaca
     3361 cctcggcgag tttgcgacca gctacggcgt ttggatctac gcgctgctgt tcttggtcat
     3421 ttttctcgag acgggcgtcg tcgtcacgcc gtttctgccc ggtgactcgc tgctgttcgc
     3481 cgtgggcgct ttggccgcgg ccggcgtcat cgacattgtc ggaattctgt cgctgctgac
     3541 ggccgcggcg atcatcggtg acaacacgaa ttacttcatc gggcgcgcag tcgggccgcg
     3601 tgtgttcacc gagcacagca aatggctgaa acgcgagcat ctcatgcgaa cacagcgctt
     3661 ctacgaggtg cacggcggca agaccgtgat catggctcgg ttcgtgccga tcgtgcgcac
     3721 gttcgcgccg ttcgttgcgg gcgtcgggcg catgcgttat ccgcggttcc tcgcgttcga
     3781 cgtcgccggc gggatcctgt gggtctgggg ctttgggctg ctcggctatt tcacgggcgc
     3841 cataccgttc gtcaaagaga acttcggcct cgttaccatc ggcgtcattc tgatttcggt
     3901 ggtgccgcta gccttcgaga ttttgcgtgc gcgcctgcag cgagcacgtg ccggcgtatg
     3961 aggtcccccc gatgacgctt ggccatgtca cgctcatcgc gcttgcggtc gcggtcgtcg
     4021 tctcatgcgt gtcgcggctc aacgtcgggc tgcttgcaat ggccctcgcg tggatcatcg
     4081 gcgtgttcat cggcggcttg cggctcgccg aggtgctcgc gggattcccg gtcgacctct
     4141 tcatcacgtt gtccggcgtc acgttgctgt tcgcgcaggc gcacgttaac ggcacgctcg
     4201 atatcgtcgc gcataatgcg atgcgcctgt gccgcggccg cgtaggcctc gtcccgatca
     4261 tgtacttcgc gctcggggcc gcgctcgcgt cggccgggcc cggcaacatc gcgacgaccg
     4321 gcttgctcgc gccgatggcg atggcgacgg cgctcaagaa ccgcacttcg ccatttctga
     4381 tggcgatcat ggtcggtaac ggctgcaacg ccggttcgtt gtcgccgctg gcaccgaccg
     4441 gcctcatcgt gaccgattac atgtcgcgca tcggcctgcc gaacttcgag ctccagacgt
     4501 gggcgtacaa cctgatggcg catgccatcg tcgcgttcgc gggttatttc gcgtttggcg
     4561 gctacaagct gctcatgcgc gcggaccgcg tcgaggtcga ggccgagccg cgcaagcagc
     4621 tcgatggcca tcagatcctg acgctcgcgg tgatcgcggt gctgatcgtc acggcgatcg
     4681 ccagccagct tttcgacatc cgcggcttca acatcggctt ggcagccatc gcgggtgccg
     4741 tgctgctcgc gctggcgcgc gcagccgacg acggccaggc gatcaagacg gtgccttggg
     4801 ccacgatcct gatggtttgc ggcgtcaccg ttctgatcgg cctgctgcag aaaaccgggg
     4861 gcatggaaat tttctcgaac ctgctgtcga gcattgcgac accggccacc gtcattcccg
     4921 agctatccgt gatcatcggc atcgtctcgg cctactcgag cacgtcgggc gtcgtgctgc
     4981 cggcgttttt gccgaccgtg ccgggcctcg tcgaaaatct aaatctcgac aatgcgctcg
     5041 gcatcgcgtc cacgatgaac atcgcgggcc acctcgtcga cgtgtcgcct ctatcgacga
     5101 tcggcgcact atgcatcgcc tccgtcccgg ccggcgagat gagccgcaag ctgttcaatc
     5161 agctcttggc gtggggcatg agcatgacgg tcgtcggcgc cgtcctctgc tgggtgctgt
     5221 tcgcctaagg cggccgcgaa gtcacagcga tcgggcgacg agcgtcctcg cgcgcgcgaa
     5281 gtcgacttgc ggcagcgagt caaccgcgtc gctgagctcg cgcaaaattt gcgcctgcac
     5341 caccccgcgc cgctgcgctt cggcgtacga aatctcgggg tggaacatca ccatcgagta
     5401 gcgcgggatg aagcgtccgg gaaagcgccg ctcgagctcg aacgacaggt cggcgcgcaa
     5461 ttggaacttc gggtcgcgca cgtcgtcgcg catctcgcga tagttctcga gcgccatctc
     5521 ggcgatcgcg tgcgcgttcg gcgcgcggtc ggcttcgaac gccgcgaaga tcgcgggcca
     5581 gtcgtggcct tcggc
//
LOCUS       DASUFX010000592         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_930650,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000592 DASUFX010000000
VERSION     DASUFX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930650"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFV99_23665"
     CDS             complement(<1..500)
                     /locus_tag="VFV99_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="catalyzes the interconversion of
                     tetrahydrodipicolinate and L,L-diaminopimelate in lysine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5062398.1"
                     /translation="MARINEHYAKLPASYLFPEIGRRVRAYQAAQPDAKVIRLGIGDV
                     TEPLAPAIVEAMHAAVDEMAKRETFKGYGPEQGYDFLVDAIRQHDYAARGVDIAADEI
                     FVSDGSKCDSGNVQEIFALAARVAITDPVYPVYVDSNVMAGRTAAAGSDGRYPGFTYL
                     VGTEANG"
     gene            557..931
                     /locus_tag="VFV99_23670"
     CDS             557..931
                     /locus_tag="VFV99_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062399.1"
                     /translation="MKWLLVLSLVACSHDVRARYPSRPDEPTGTLVLAMSRSASGVSV
                     AINGLLVVDDAKTDHVVIEGVPVGTREVVMAANGSDKAFNVWIDTEHPTTVPLGVPDE
                     GSGFLKSLAGSLISILVYSYLH"
     gene            complement(932..1366)
                     /locus_tag="VFV99_23675"
     CDS             complement(932..1366)
                     /locus_tag="VFV99_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062400.1"
                     /translation="MRGVWIVVVVACSKPPPPKAPVVERPADQVADIQGRWISSDELD
                     NSYTMTIEANGTIDVWIDRGKTGRCEQKGKIVAGEASRTFKVTYKLGECNPQAVNTPI
                     DMSVKSFTGDELTIVVFDQPRSYTRVAEQEPRGERGAPAQLQ"
     gene            1472..3727
                     /locus_tag="VFV99_23680"
     CDS             1472..3727
                     /locus_tag="VFV99_23680"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX5062401.1"
                     /translation="MTMRSDRNDFIRDIIDEDLRTGRHTNVATRFPPEPNGYLHIGHA
                     KAICVDFGVARDYQGTCNLRYDDTNPTKEDIEYVESIERDVRWLGFTPSAVLFSADYF
                     PKMYELAERLVREGKAFVDDLTDEEIKEYRGTLSEPGKPSPYRDRSPEENLDLLRRMK
                     AGEFPDGAKVLRAKIDLASANMKMRDPLLYRIRHAHHHRTGDQWCIYPMYDYAHPLED
                     AIEGITHSICTLEFENNRELYDWVLDNTGPWNPRPHQYEMARLALEYTLMSKRKLRQL
                     VEEGIVRGWDDPRMPTLAALRRRGYTPEAIRTFCDMIGVAKANSMVDIGKLEYCVRDD
                     LNKIAPRVLGVLRPIEVEVSGEVPAASTDAPYFPPDVGKPGSRTLAISNRIFIDRDDW
                     SENPPADWKRLAPGRTVRLRHAYGISVEEVVARDAAGNATKLRAKIVDPGKGAGVIHW
                     VDVATSVPAEIRLYERLFKVPKPEEGGGDPLVHLDRNSLEVVTTARVEASLANAAVGS
                     RWQLERVGYFIVDEDTKPGALVLNRIITLRDEKPTVVTIAPTERKANVKAATRPKGKS
                     PAEYRAEARARDVDLADAYRKAQAMGLSDEQADLLTGERASAKLFVETATATNDAPLV
                     AKWMINELPRALGDRELADAGLDAGRFAELVALLRDGTITPAVGKTVLAQMVQTGKRA
                     HELAAAHTATSTIDLGSAVDAVIAANPDKAAQYKAGKTGLLGFFVGQVMKSTPNADAA
                     AVNRVVRERLG"
     gene            3805..4629
                     /locus_tag="VFV99_23685"
     CDS             3805..4629
                     /locus_tag="VFV99_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062402.1"
                     /translation="MTWKLIAIGVLAVGCGAQLGDGVDEHGISVDASTGGGGGGGGGG
                     GGGGGGGGGGMHDAGVPVGPDAPPGSACGNNRKVFLEFNGVTITKATTSDATLNYASW
                     ISANAVVPPWHQGSSTRTTDITTIVNGVKSRLANTPIQVVTTRPAAGPYVMIVFGGQS
                     TGNGGTVGTTYGGSTNEHDCGDLVKSDLGWISDNPSVSYAQDLAIGAIGWGLGLNGTN
                     DPNDCMCAWANNCQSASGACTLSASIATTTSSSPATTCPNQNPQNEVAAFTTKFCQ"
     gene            4679..>5341
                     /locus_tag="VFV99_23690"
     CDS             4679..>5341
                     /locus_tag="VFV99_23690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062403.1"
                     /translation="MIGTTVGSYRVTAKVSVGGMGTVYKAEHSLIGKLAAVKVLHPEL
                     RGNREVVQRFFNEAKATTQIKHPGIVEIFDFGYLPSGDGFIVMEFLEGVSLARRLKKF
                     PKMPEGEAVVLMRSVCSALAAAHAKGIVHRDLKPDNIFLCPDSDAPTGERPKILDFGI
                     AKLNVGLTGPGNATKTGAVMGTPTYMSPEQCRGTGEVDHRADLYSIGCMVYEMVAGRP
                     PFD"
BASE COUNT          976 a         1713 c         1735 g          917 t
ORIGIN      
        1 ccgttggcct cggtgccgac gaggtacgtg aagccggggt agcggccatc gctgcccgcg
       61 gccgccgtac gtcctgccat cacgttcgaa tcgacgtaca ccgggtacac aggatcggtg
      121 atcgcgacgc gcgcggccag cgcaaagatc tcctggacgt taccgctgtc acacttgctg
      181 ccgtcgctga cgaagatctc gtcggcggca atgtcgacgc cgcgcgccgc gtagtcgtgc
      241 tggcggatcg cgtcgacgag gaagtcatag ccctgctcgg ggccatagcc tttaaacgtc
      301 tcgcgctttg ccatctcgtc gaccgcggca tgcattgcct cgacgatcgc gggcgcgagc
      361 ggctcggtga cgtcaccgat gccgagccgg atcaccttcg cgtccggttg cgctgcctgg
      421 taggcgcgca cgcgacgacc gatttcggga aacaggtagc tggcaggaag ctttgcgtag
      481 tgctcgttga tacgggccat gacggcgaga acgtagccga gcaacctttc taccgccagc
      541 gatcgtctga cttgccgtga agtggttgtt ggttcttagc ctggtggctt gttcgcatga
      601 cgtgcgtgcc cggtatccgt ctcgccccga cgagccgacg gggacgctcg tgctcgcgat
      661 gtcccgttcc gcgtcgggtg tcagcgtcgc gatcaacggc ctgttggtcg tcgacgacgc
      721 gaagacagac cacgtcgtga tcgagggtgt cccagtcggc acgcgggagg tggtgatggc
      781 agcaaacggc agcgacaagg cgttcaatgt ctggatcgac accgagcatc cgacgacggt
      841 cccactcggc gtccccgacg aaggcagcgg ctttctcaag tcactcgccg gctcgctgat
      901 ctcgatcctc gtctactcgt acttgcactg actactgcag ctgcgccggc gcgccgcgtt
      961 caccgcgcgg ctcctgctcg gccacgcgcg tgtacgagcg cggctgatcg aaaacgacaa
     1021 tcgtgagctc atcgccggtg aacgacttca cgctcatgtc gatcggcgtg ttgactgctt
     1081 gcggattgca ctcgccgagc ttgtacgtga ccttgaatgt acgactcgcc tctccggcga
     1141 cgatcttgcc cttctgctcg cagcggccgg tcttcccgcg atcgatccag acgtcgatgg
     1201 tcccgttcgc ctcgatcgtc atcgtgtagc tgttgtcgag ctcgtcgctg gagatccagc
     1261 gaccttgaat gtctgcgacc tgatcggccg ggcgctccac cacaggtgcc tttggcggcg
     1321 gcggtttgct gcacgccacc acgacaacaa tccacacgcc gcgcatggct ggaccgtaca
     1381 cgtgaacaag gcgtgaagcg accccacctg atggggtgtg cacgcattga gtgtcggcgc
     1441 gccccctgtg gggtagagtt cgcgcgctgt catgacaatg cgctctgatc gcaacgactt
     1501 catccgcgac atcatcgacg aggacctgcg tacgggacgg cacaccaacg tcgcgacgcg
     1561 gttcccgccc gagccgaacg gctatctcca catcggccac gcgaaagcaa tctgcgtgga
     1621 cttcggtgtc gcgcgcgatt accagggcac gtgcaacctc cgatacgacg acacgaaccc
     1681 gacgaaggaa gatatcgagt acgtcgaatc gatcgagcgc gacgtgcgct ggctcggctt
     1741 tacaccgagc gctgtgctgt tctccgccga ttactttccg aagatgtacg aattggccga
     1801 gcggctcgtt cgcgagggca aggcgttcgt cgacgatctg accgacgagg agatcaagga
     1861 ataccgcggc acgttgagtg agccgggcaa gcccagcccg taccgcgatc gctcgcccga
     1921 agagaatctc gacttgctcc gccgcatgaa agcaggcgag ttccctgacg gcgcgaaggt
     1981 gctgcgcgcg aagatcgatc tcgcgagcgc gaacatgaag atgcgcgatc cgctgctcta
     2041 tcggatccgc catgcgcacc atcaccggac cggcgatcag tggtgcatct acccgatgta
     2101 cgactacgcg catccgctcg aggacgcgat cgaaggcatc acgcactcga tctgcacgct
     2161 cgagttcgag aacaaccgcg agctgtacga ctgggtgctc gacaacaccg ggccatggaa
     2221 tccgcgtccc catcagtacg agatggcgcg tctcgcgctc gagtacacgc tgatgagcaa
     2281 gcgcaagctg cgccagctcg tcgaggaagg catcgtgcgc ggctgggatg atccgcgcat
     2341 gccgacgctc gccgcattgc gccgccgcgg ctacacgccc gaagcgatcc gcacgttctg
     2401 cgacatgatc ggcgtcgcga aagcgaactc gatggtcgat atcggcaagc tcgagtactg
     2461 cgtgcgcgac gatctgaaca agatcgcgcc gcgcgtactc ggtgtgttgc gaccgatcga
     2521 ggtcgaggtg agcggcgagg tgccagctgc gtcgaccgac gcgccgtatt tcccgcccga
     2581 cgttggcaag ccgggctcgc gcacgctggc gatcagcaat cgcatcttca tcgatcgcga
     2641 cgactggagc gagaatccac cggccgattg gaagcggctc gcgcctggtc gcacggtgcg
     2701 gttgcgccac gcatacggaa tctccgtcga ggaggtcgtc gcgcgtgacg ctgccggcaa
     2761 tgcaacgaag ctgcgcgcga agatcgtcga tccgggcaag ggtgccggcg tgatccactg
     2821 ggtcgatgtc gcgaccagcg tgcccgcgga gatccggctg tacgagcggt tgttcaaggt
     2881 gccgaagccg gaagaaggcg gcggcgatcc gctcgtccac ctcgatcgca attcgctcga
     2941 ggtcgtcacg accgcgcgcg tcgaagcatc gctcgcgaat gcggcggttg gttcgcgctg
     3001 gcagctcgag cgcgtcggct acttcatcgt cgacgaggac acgaaaccgg gtgcgctcgt
     3061 gctcaaccgc atcatcacgt tgcgcgacga gaagccgacg gtcgtgacca tcgcgccgac
     3121 cgaacgcaag gcgaacgtga aggcggcgac gcggccgaaa ggcaagtcgc cggcggagta
     3181 ccgcgccgaa gcgcgcgcgc gcgatgtcga tctcgccgac gcgtaccgca aggcgcaagc
     3241 gatgggcctc tccgacgagc aggccgatct gctcaccggc gaacgcgcga gcgcgaagct
     3301 gttcgtcgag accgccacgg cgacgaacga cgccccgctg gtcgcgaaat ggatgatcaa
     3361 cgagctgccc cgcgcgctcg gcgacagaga gcttgccgat gccggactcg atgcgggccg
     3421 gttcgcagag ctcgttgcgc tcttgcggga tgggaccatt acccccgccg tcggaaagac
     3481 cgtccttgcc cagatggtcc agaccggtaa gcgcgcccac gagctcgcgg ccgcgcatac
     3541 ggcgacgtcg accatcgatc tcggctccgc ggtcgacgcg gtgatcgctg caaacccaga
     3601 caaagcggcc cagtacaaag cgggtaagac cggcttgctc ggcttctttg tcggccaggt
     3661 catgaagtcg acgccgaacg ccgacgccgc cgcggtcaat cgcgtggtcc gggagcgact
     3721 gggctaacga cctggggcag aagtctccaa tctgaggatt taatcccagt tttctccagc
     3781 acttacgatc gctacaagta gatcgtgacc tggaagctga tcgccatcgg tgtgctcgca
     3841 gttggctgtg gtgcccaact cggcgacggc gtcgacgagc acggcatcag tgtcgatgcc
     3901 tcgaccggcg gcggcggcgg tggtggtggt ggcggtggcg gaggaggcgg cggtggtggc
     3961 ggcggcatgc acgacgctgg cgttccggtc gggcccgatg cgccgcccgg gtcggcgtgc
     4021 ggcaacaacc gcaaggtctt tctcgaattc aacggcgtga cgatcaccaa agcgacgacg
     4081 agcgacgcga cgctgaatta cgcgagctgg atcagcgcga acgcggtcgt cccgccgtgg
     4141 catcaaggat cgtcgacgcg cacgaccgac atcacgacga tcgtcaacgg cgtgaagtcg
     4201 cggctcgcga acacgccgat ccaggtcgtc accacgcgac cagcggcggg tccgtacgtg
     4261 atgatcgtgt tcggcggtca gagcaccggc aacggcggca cggtcggcac gacctacggt
     4321 ggctcgacga acgagcacga ctgcggcgat ctcgtgaaga gcgacctcgg ttggatctcg
     4381 gataacccgt ccgtgtcgta tgcacaggat ctggcaatcg gcgcgatcgg ctggggtctc
     4441 ggcctgaacg gcacgaacga tcccaacgac tgcatgtgcg cctgggcgaa caactgccaa
     4501 tcggcttctg gtgcttgcac gctatctgcc tcgatcgcga cgacgactag ctcctcgccg
     4561 gcgaccacct gccctaatca gaacccgcag aacgaggtcg ccgccttcac gacgaagttc
     4621 tgccagtagg cccgtgtagg tgcgatctcg tcgacgccgt ttgttatgat cagcggtgat
     4681 gatcgggacg accgtcggca gctaccgcgt cactgccaaa gtgagcgtcg gtggcatggg
     4741 cacggtgtac aaagccgagc actcgctgat cggtaagctc gcggccgtca aggttctgca
     4801 tcccgagctc cgcggcaatc gcgaggtcgt gcagcgcttc ttcaatgaag cgaaggcgac
     4861 gacgcagatc aagcatccgg gcatcgtcga gatcttcgac ttcggctact tgccgagcgg
     4921 cgatggcttc atcgtcatgg agtttctcga aggcgtctcg ctcgcgcgcc gcctcaagaa
     4981 gttcccgaag atgccggaag gcgaagcggt cgtgctcatg cgcagcgtct gcagcgcgct
     5041 cgcggccgcg catgcgaaag gcatcgttca tcgcgatctg aagcccgaca acatcttcct
     5101 ctgcccggac tccgacgcgc cgaccggcga gcgaccgaag atcctcgact tcggtatcgc
     5161 gaagctcaac gtcgggctga caggccccgg caacgcgacg aagaccggcg cggtgatggg
     5221 cacgccgacg tatatgtccc ccgagcagtg ccgcggcact ggcgaagtcg atcatcgcgc
     5281 ggacctctac tcgatcggct gcatggtcta cgagatggtc gccgggcggc cgccattcga
     5341 t
//
LOCUS       DASUFX010000593        16182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_791372,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000593 DASUFX010000000
VERSION     DASUFX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791372"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1654
                     /locus_tag="VFV99_23695"
     CDS             17..1654
                     /locus_tag="VFV99_23695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062404.1"
                     /translation="MKVCTGCATKTDETGRFCPECGAPLVEAGRSVIGRATTQPSDPN
                     ATDVLPQSKKPESSSASKLIGLTIDGQFEIDGILGGGAFGTVYRGRQNGLDRPVAIKV
                     PTSEIAADPVMARRFAREARSAARIQHPGVVAIYAVGELADGRPYLAMQYVEGEPLDK
                     VLADGPLSPVRALRIARQIASALSETHVADVVHRDLKPTNIMWRRDRNGDDRITIVDF
                     GIAVCKPGNADATRLTAGGLIGTPHYMSPEQAHGEQVDARADLYALGCLLFELVTGAP
                     PFEGSGFEVLLAHLGRPAPLPSEKNPNVPEAVDRICAMLMRKKPDDRPQHADEVVALI
                     DEALAELAGKEPATAPAPMKKRKSKKRPTIATLRDLPEGTELPHQRRSRRMLLAALAV
                     IMLVGGAGFAVYQMREKPALAIEPDPDDAPADSDPRVGRRRTLTKDNGEMIVTAMVPD
                     PLVAGQEIRSRLEIKNKLGQPVVADEIVLTIGDSTGAAKGLTARPRRTTGANASATAG
                     APGRYYFRYTFPAKGHYTLRVFPPSIDSSFEIPVDVE"
     gene            complement(1705..2247)
                     /locus_tag="VFV99_23700"
     CDS             complement(1705..2247)
                     /locus_tag="VFV99_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062405.1"
                     /translation="MSLRTIFVSSLLVIGLLGGSALAGDPNQVFKGKIMLSTKRFPLS
                     AKSKDAYIAAVRKQSSSNFQENKENHTWKIYFAAFLSKPLNDVEYVIKFYELGNGSQQ
                     LLGASEAFNDERGQKTIVSNITLEKKSFGVNKQLLMTIENKGSVLASARFKILGEGER
                     FTGKVNFSEDEANGKSNGDE"
     gene            complement(2267..3157)
                     /locus_tag="VFV99_23705"
     CDS             complement(2267..3157)
                     /locus_tag="VFV99_23705"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX5062406.1"
                     /translation="MTARNPTTFLVGAGPVATALAGALRLGGVPVLGLWGRTPSAARA
                     AGSTAGVAAFSSAPPDILLEAEVVILAVRDASIGEAAQTLVGTGLIGKRHVMLHCAGA
                     TSAKEALGNVTGAVGGIGTLHPLMAIADGKTAMRALKGTVFGVEGDDAGRAMATKLVN
                     ALGGIVLALDSSQMGAYHCAAALASNYIVAAIDAAAQVLAGAGVSPTQAAQALVPLAE
                     GALHNVREKGTTAGLTGPVRRGDAVTIQRHLDALREHPDLAKLYRALARHAVEIATRI
                     DGPDAPDRAGLDAIRRLVET"
     gene            complement(3164..3736)
                     /locus_tag="VFV99_23710"
     CDS             complement(3164..3736)
                     /locus_tag="VFV99_23710"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEX5062407.1"
                     /translation="MRRVGLFGGSFNPPHVAHQLVALYVLETVVDEVWFVPTYSHPFG
                     KALAAYDHRVAMCELAVAPLGARAQVSRAEHDLAQKPDFVASRTLDLIGHFIAKHTDA
                     TFHLVVGADILGETDKWHRWDEVSRLAPPIVLGRSGYPLPVGSVETGVVMPEISATRI
                     RDLLANQDPGVAALLPQTVLRYIERHGLYA"
     gene            complement(3733..4911)
                     /locus_tag="VFV99_23715"
     CDS             complement(3733..4911)
                     /locus_tag="VFV99_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX5062408.1"
                     /translation="MAIETGLARLAAQGSELAPFTRLGLIVNATSVDRELRHAIDLLT
                     GRKDLQVTALFGPEHGVRGDAQDMIGVDAATDARTGLPVYSLYGHSEASLSPTPEMLE
                     NVDALVFDIQDVGSRYYTFVWTMVLAMRACAKAKKGFVVLDRPNPIGGTHIEGPAITP
                     GFESFVGLVSCPNRHGLTAGEIARWRAEVEKLDLELVVISMRGWERDMWFEHTGLPWV
                     LPSPNMPTVDTALVYPGMCLVEGTELSEARGTTRPFELSGAPHIDGDRLARDMAAMNL
                     PGAKLRPVVYTPTFHKHAFKPCGGVQIHVTDADAFRPYRTGIAFLKACHDQAPAQFKW
                     REKAYEFVDAIPAIDLLAGNDTIRKGIEAGVSLDELAERWPRDEGAFAEERAPYLLYK
                     "
     gene            complement(4918..6423)
                     /locus_tag="VFV99_23720"
     CDS             complement(4918..6423)
                     /locus_tag="VFV99_23720"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX5062409.1"
                     /translation="MSRTVIAAVVAVVIAALTAIAFFVTKTSFEDKIRKDAEAQLRRS
                     YQVVQQLAQLEAIDVANKAERLAADKAFVLALKSESAMERANQARVGMTKFTSNEKEG
                     DVKPDMIALVDANGLLVAMNDVASVVPKQFQKEGAKPGETTIPALNVVLGSRVIISDI
                     WNDRGRMMKIGVAPIIDADAPVPANDPDGVVIIGAVIVAYAQTAQQAQMDKRLLGTEI
                     AYYDQQRVVATSFTKGGTNEEDTAKATQLSELVRSGKIKEGSDKPTKVSIDGVTYYGA
                     AVQMPRSTTNARRMPQDYPAVTAGAMVLASAGDSVIAGGIKMFIILLGLGALAISMLG
                     LYLTHRRLVAQVDQIELGVTDIINGNVDRTFRPVGAELDGLANGLNVMLARLLGRPEP
                     GEEEFDEEGNPIIPGRVDFEEGEEKPAVDPDLANLAKESEPDYYKRVFTEYVAARKAA
                     GSPDDVSFENFIAKLKVNEGKLKAQYNCRAVRFRVVTKDGKVSLKPVPIFE"
     gene            complement(6481..8145)
                     /locus_tag="VFV99_23725"
     CDS             complement(6481..8145)
                     /locus_tag="VFV99_23725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062410.1"
                     /translation="MKCPSCQNDVLDIDVKCPHCGTALGATGAHRMIGSLILGTYELV
                     DVLGQGGMSVVFKARHKMTDQEVALKILPPELAAHSQVKSRFLDEAKALAALDHPNIV
                     HLYNFGQENGSFVLAMQFVQGRTWERLILENKRLDWKASCKITIDVLKALEYAHGRGV
                     VHRDMKPSNVLVRESDGAATVMDFGIAKMAQSTRLTATGQTMGTVRYMSPEQVRGQEV
                     DLRTDIYSLGATLYESLVGDTPFDGSTHFEIMTKHLSEIAKPPSVVGIEVPRAVEDAL
                     MRCLAKKAPDRFDSARDMRKVLEGALKEADVALVETQRFSRDLIDVRATKDERPPVRA
                     AVAVAETVRAATANDLADELEPGTTGLPARKKRGSKWPVIAVAVLVLGGGATAAALLM
                     GDNKPAGFKAATKIKGLTEGKTFPDGLVVETDGKVSAGEVEAAYRLVLEQLKGFEPDV
                     QARRDVIQEIVALPQATLCEPRAAITPASTQPCTESDGFTTTLRGEKRQLLIADDRGT
                     LAQTMRLGVARAVCDFQFLYLNSNNEDDVKRLDTICEMTKRFGTAK"
     gene            complement(8142..9047)
                     /locus_tag="VFV99_23730"
     CDS             complement(8142..9047)
                     /locus_tag="VFV99_23730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5062411.1"
                     /translation="MVPGIQQRHVVARDGTRIGYQVRGAPSRPCIVFANGLGGTYIAF
                     RYLYSALDDAYQTLCWDYRGLYTSDASKDPNANTVVHQVDDLVAILDQEKVDSAVIVG
                     WSMGVQVGFEAIRRYPERVRGMLAINGTYGRAFRTVMGSRLVGRTIPMLLRLVKAQAA
                     LAGYATKRLAGSDALIAAMKRVGLVSQTIDIEIFRAVAAGFQTIDWRIYSDLLTRLDE
                     HDAEEVLPQIKVPISIVTGDRDLMTPPSTAEHMHRTIPGSRMVVIEGGTHYTPVEYPA
                     ILVDELGRLLDRIPGWQRRASEATA"
     gene            complement(9120..9998)
                     /locus_tag="VFV99_23735"
     CDS             complement(9120..9998)
                     /locus_tag="VFV99_23735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5062412.1"
                     /translation="MNVRLPLVLGALVASSLGVSGVAEAQRVRFSGGVRWSGGVSVTS
                     GSWHFARPAWSPSRWHVRGSIYVGPTYRYYPRPYYVYYPAYVPSYYESTSYYPVQPAA
                     VQAPGVVAVTPVREPLPRLGIGLFAGGSAVESQTGDSTHESDDLGLLGRFRLTPGLII
                     EGEIGKMTYDVDGVENARVDRRLGASLLYEIGAYNRWAPYILVGTGVQQAEVGGEFTT
                     TQDYGEIGAGLRWAVTPRFHLALEFRAGSRGTVASDEPMLTPAGTTGRVITPPSASSD
                     ETEEDTRGRLNAILYF"
     gene            10275..11519
                     /locus_tag="VFV99_23740"
     CDS             10275..11519
                     /locus_tag="VFV99_23740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062413.1"
                     /translation="MSDAKKAGSRDISELKQRLGLKKGGAAQPAAGGRSNGAPSGGVV
                     APPGLAVPQAPPQPAAPNAHDDPFGAMNHMAAVAQVQRAPEKEYIVVNDGNVEKVHAA
                     SHGALIAKIAVPAGIALIVGIAIGKIGTGASNYNEGLRGARVVLGDKTSGSTVAMLKK
                     QLSDLDNLLDEAKTKNGFKPSLELDKKLKEAAAKLEVKTEVVFRAKQIDPELSGQVLS
                     FYAGVLEVKDMIDIHNKAAAGDDIMLKKAKDAADKAATDDVPGGLRFAVLLSAPTETD
                     RNEFGAKLVEIAGVYCGAGNNPVSRCPEGEFPSAYAYRNEPGATPIKGDLVPTGSDTL
                     PGKKIVPLLSNGVRDSFIKGGEPTISEIYYTKRLRALYDRIHGKVGQDGKPQGGLIED
                     GNKLETRLTTEAGKSTRFSFFM"
     gene            11555..12466
                     /locus_tag="VFV99_23745"
     CDS             11555..12466
                     /locus_tag="VFV99_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062414.1"
                     /translation="MRRIRTALVIATLVGFGLQIPAALAADAKKAAPAKKAPAKSAKA
                     KATPPKKKEPPPMVNAEHKKALAEKFAGFKFGMTKDEVLAVLQKQINERFEEKIKATT
                     DVAMQDRYRKDKKAELARTSSTYVEFNGKKGGWDVSIVENEFAHNTGESMMERWENDG
                     GKNQRRFFFFYNQRLWKMFISLDVSMIPDDKRNFDTFKGVMEGQYGKGGVDGGTIAWR
                     AGEFDVRAIDRLKDYGALGLVLEDPKVRSEVVALRESKAPPKQETPSVIKAIIDPDNK
                     DHPDVKSNSGAVEAVIQGNGAATPPKK"
     gene            12536..13705
                     /locus_tag="VFV99_23750"
     CDS             12536..13705
                     /locus_tag="VFV99_23750"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX5062415.1"
                     /translation="MKRRTFLRGSAAGIAIAGAHWLRVPTLVRADDKPAAAGDSAGQL
                     YELTSRPANYESTRDTFTTRITPVERFYIRNHFDIPVGDMKDWKLELKGLVAKPQTFT
                     LADLEKMKQTTVEAVLQCAGNGRGWFVPRVPGVQWRWGAMGNAEWTGVRLKDLLAAAG
                     VAKEAQHVQLQGAEKPTLEKTPAFIRGIPIAKAMHPDTLVALRMNGQPLSPHHGYPAR
                     LVVPGWVGDDWMKWLATIEVRADEPKGFFYETGYRFPATPGKPGEPVPPDQMKPMTKL
                     VVKSQLGSIGDGDVIAPGPHELVGVAFSGEAKIKKVDITVDGGATWQPAKLDAHDSGY
                     GFRLFRYTWDAKPGKTKVGARATDSAGAVQPATAVWNPAGYLYNAIEMVEVEVRA"
     gene            13702..14118
                     /locus_tag="VFV99_23755"
     CDS             13702..14118
                     /locus_tag="VFV99_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5062416.1"
                     /translation="MSRFVIIVLALAACRSEDKPAPAQQPVPTQPTPPAAPPVTIDRD
                     ALLAGKLPEGAPETDLVNAQCRICHSVEYLTQQRLGEAAWKKTIDKMRKFGAVLTDEQ
                     AASLVAFAAHYWNPDLPGRTWTPGPPPPGALPITTK"
     gene            14278..15045
                     /locus_tag="VFV99_23760"
     CDS             14278..15045
                     /locus_tag="VFV99_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062417.1"
                     /translation="MKTLNWLLAAGLMTLVGACAEGGDDDLSDASELSLSANAFSHTK
                     IVGDVDLGFSRSVLYKSSPRYRAVRFHAEAGTMLEVYVRSTHGDPTTWLTDVNFKVIA
                     HNDDANEETSSNISIESLAKTGDYYVVFRDKHYESHYFDVAPVQLDLPAHHPMVPDVE
                     TIYEALVASHTLATKQISGDGLPFLAKGLYARFTSEAAAEGAQAAAYAIEVDGETVWL
                     VRKYKAGEYLVVGAYVESGPMLGIAGGTSELIDNWEY"
     gene            15063..15497
                     /locus_tag="VFV99_23765"
     CDS             15063..15497
                     /locus_tag="VFV99_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062418.1"
                     /translation="MDEIEAELTAFGDPARNIRDDAFEALHDRVFPRGTHGELAWTVG
                     VRIAPRVIELATRPEGAWAAILLLDLVAAARRGGHRKVLDAAAPLAIADFSAVAPAVR
                     AALELARQLAIGGPMPSDEVITNAVDDAVPDENIAALPEHQK"
     gene            15563..>16182
                     /locus_tag="VFV99_23770"
     CDS             15563..>16182
                     /locus_tag="VFV99_23770"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062419.1"
                     /translation="MARSPAETPLMRQYLDLKEKHPDALLFFRCGDFYEMFFEDAVIA
                     ARALDLVLTSRDKGKEDSVPMCGVPYHAARGYLAKLTELGHKVAVCEQMEDPKLAKGL
                     VKREIVRVITPGVVLDDDVLDPKLPRYLAALVPGDRGKSTVKARPDEVLVGLAYLDAT
                     TGDLFATEIAMSAVLDELIRVSPRELLADPSVLGAERNPLAVLQQR"
BASE COUNT         2820 a         5329 c         5337 g         2696 t
ORIGIN      
        1 gctacgttca acatttgtga aggtctgcac gggctgcgcg acgaaaaccg acgaaacggg
       61 acggttctgt cccgagtgcg gagctccgct cgtcgaggcc ggtcgcagcg tcatcggtcg
      121 ggcgacgacg cagccgagtg acccgaacgc caccgacgtg ctgccgcagt cgaagaagcc
      181 cgagtcgtcg tcggcgagca agttgatcgg cctgacgatc gacggtcagt tcgagatcga
      241 tggcatcctc ggcggcggcg cgttcggcac cgtgtatcgc ggccgccaaa acgggctcga
      301 tcggccggtc gcgatcaaag tgccgacgag cgagatcgca gccgatcctg tgatggcgcg
      361 gcggttcgca cgcgaggctc gctccgcggc gcgcatccag catccaggcg tcgtcgcgat
      421 ctacgcggtc ggcgagctcg ccgatggccg gccatacctc gcaatgcaat acgtcgaggg
      481 cgagccgctc gacaaggtgc tcgccgacgg tccactgtcg ccagtgcgtg cgctgcgcat
      541 cgcgcgtcag atcgcgagcg cgctatccga gacgcacgtc gcggacgtcg tgcaccgcga
      601 tctcaagccg acgaacatca tgtggcgccg tgatcgcaac ggcgatgatc ggatcacgat
      661 cgtcgacttc ggcatcgcgg tctgcaagcc gggcaacgcc gatgcgacgc gcctcaccgc
      721 cggcggtttg atcggcacgc cgcactacat gtcgcccgag caggcgcacg gcgagcaggt
      781 cgacgcgcgt gcggatctct acgcgctcgg ctgcctcttg ttcgagctcg tcaccggcgc
      841 gccgccgttc gaaggctcgg gcttcgaagt gttgctcgcg catctcggcc gccctgcccc
      901 gctgccgagc gagaagaatc cgaacgtgcc cgaggcggtc gatcgcatct gcgcgatgct
      961 gatgcgcaag aagcccgacg atcgcccgca gcatgccgat gaagtcgtcg cgctgatcga
     1021 cgaagcgctc gccgagctcg ccggcaagga gcccgcaaca gcgccggcgc cgatgaaaaa
     1081 gcgcaagtcg aagaagcgcc cgacgatcgc gacgctgcgc gacctgcccg agggcaccga
     1141 gctaccgcac cagcgccgct cgcgccgcat gctgctcgcc gcgctcgccg tgatcatgct
     1201 cgtcggcggc gccggctttg cggtctatca gatgcgcgag aagccggcgc tcgcgatcga
     1261 gcccgacccg gacgatgccc cggccgatag cgatccgcgc gtcggccgtc gccgcacgct
     1321 caccaaggac aacggcgaga tgatcgtgac ggcgatggtg cccgatccgc tcgtcgcggg
     1381 tcaggagatc cgctctcgcc tcgagatcaa gaacaagctc ggtcagccgg tcgtcgccga
     1441 cgagatcgtg ctgacgatcg gcgattcgac cggtgccgcg aagggcctca ccgcccggcc
     1501 gcgtcgcacg accggggcga atgcgagtgc gacggcaggc gcgccgggcc gctactactt
     1561 ccggtacacg ttcccggcca agggccacta cacgctgcgc gtgttcccgc cctcgatcga
     1621 cagctcgttc gagattccgg tcgacgtcga atgaaaggcc tcgggcctcc cactgaaggc
     1681 tgagggcctg aggcctgctc gagcttactc gtcgccgttc gacttgccgt tcgcttcgtc
     1741 ctccgagaag ttcaccttgc cggtgaaccg ctcgccctca cccaggatct tgaagcgcgc
     1801 gctcgcgagc acgctgccct tgttctcgat cgtcatcaac agctgcttgt tcacaccgaa
     1861 cgacttcttc tcgagcgtga tgttcgagac gatcgtcttc tgaccgcgtt cgtcgttgaa
     1921 cgcttcgctc gcgccgagca gctgctggct gccgttaccg agctcgtaga acttgatgac
     1981 gtactcgacg tcattgagcg gcttcgacag gaatgccgcg aagtagatct tccaggtgtg
     2041 attctctttg ttctcctgga agttcgagct cgattgcttg cgaacggccg cgatgtacgc
     2101 gtccttgctc ttcgccgaca gcgggaagcg cttggtcgag agcatgatct tccctttgaa
     2161 gacctggttc ggatcgccgg caagcgccga acctccgagc aagccgatga cgagtaacga
     2221 cgagacaaaa atcgtgcgaa ggctcatcgt cctcgatggt acacgatcac gtctcgacga
     2281 gtctgcgaat cgcgtcgagt ccggcgcggt cgggagcatc cggaccgtcg atgcgcgtgg
     2341 cgatctcgac cgcatgacgc gcaagcgcgc gatacagctt ggcaaggtcg ggatgctcac
     2401 gaagtgcatc gagatgacgt tgaatggtta cggcatcccc acgtcgaacc gggccggtga
     2461 ggcccgccgt cgtgcccttc tcacgcacat tgtgaagcgc accttcagcg agcggcacga
     2521 gcgcttgcgc cgcctgcgta ggcgacacgc ccgcacctgc gagcacctgc gcggccgcat
     2581 cgatcgcggc gacaatgtaa ttcgatgcca gcgccgcggc acagtgatac gcgcccatct
     2641 gcgagctgtc gagcgcgagc acgatgccgc cgagtgcgtt gacgagcttc gtcgccatcg
     2701 cgcggcccgc gtcatcaccc tcgacgccga acacggtgcc cttgagcgcg cgcatcgcag
     2761 tcttgccgtc cgcgattgcc atcagcggat gcagcgtgcc gatgccgccg acggcacccg
     2821 tgacattgcc gagcgcttcc ttcgcgctcg tcgcgcctgc gcagtgcagc atgacgtggc
     2881 gcttgccgat gaggccggtg ccgacgagcg tctgcgcggc ttcgccgatc gatgcgtcgc
     2941 gcacggcgag gatcacgacc tcggcttcga gcaagatgtc gggtggcgcg gacgagaacg
     3001 cggcgacgcc ggccgttgag cccgcggcac gcgctgccga cggcgtgcgg ccccacaggc
     3061 cgagcactgg aacgccgccg agccggagcg cgccggcgag cgcggtggcg acgggtccag
     3121 cgccgacgag gaacgtagtt gggttgcgtg cggtcattgc ggtctaggcg tagaggccgt
     3181 ggcgctcgat ataccgcagg accgtctgcg gcaaaagcgc ggcgacgccg gggtcctggt
     3241 ttgccagcaa gtcgcgaatc cgcgtcgctg aaatctcggg catcaccacg ccagtttcga
     3301 cacttccgac cggtagcggg tagccgctgc ggccgagcac gatcggtggc gcgagccggc
     3361 tgacctcgtc ccagcgatgc cacttgtccg tctcgccgag gatgtcagcg ccgacgacga
     3421 gatgaaacgt tgcgtcggtg tgcttcgcga tgaagtgccc gatcagatcg agcgtgcggc
     3481 tcgcgacaaa gtccggcttc tgcgcgaggt cgtgctccgc gcgactgacc tgcgcgcgcg
     3541 caccgagcgg cgcgactgcg agctcgcaca tcgcgacgcg atgatcgtac gcggcgagcg
     3601 ccttcccgaa cggatggctg tacgtcggca cgaaccacac ctcgtcgacc acggtctcga
     3661 gcacgtagag cgcgacgagc tggtgtgcga cgtgcggcgg attgaagctg ccgccgaaca
     3721 gaccaacacg tctcatttgt acaagaggta cggtgccctc tcctccgcga atgcgccttc
     3781 gtcgcgcggc cagcgctcgg caagctcgtc gaggctcacg cccgcttcga tgcctttgcg
     3841 aatcgtatcg ttgccggcga gcaaatcgat cgctggaatc gcatcgacga actcgtacgc
     3901 tttttcgcgc cacttgaact gcgccggcgc ctggtcgtga catgccttga gaaacgcgat
     3961 gccggtgcgg tacggacgaa acgcgtcggc gtcggtgacg tggatctgta cgcccccgca
     4021 cggcttgaac gcgtgcttgt gaaacgtcgg cgtgtagacg accggccgca gcttcgcgcc
     4081 tggcagattc atcgcggcca tgtcgcgcgc gagccgatca ccgtcgatgt ggggcgcgcc
     4141 cgacagctcg aacggccgcg tcgtgccgcg cgcttccgac agctcggttc cctcgacgag
     4201 gcacatgccg gggtacacga gcgcggtatc gaccgtcggc atgttcggcg acggcagcac
     4261 ccacggcagc ccggtgtgct cgaaccacat gtcgcgctcc cagccgcgca tcgagatcac
     4321 gacgagctcg agatcgagct tctcgacctc ggcgcgccag cgtgcgatct cgccggccgt
     4381 cagaccgtgg cgattcgggc acgacacgag gccgacgaac gattcgaagc ccggcgtgat
     4441 cgccgggccc tcgatatgcg tgccgccgat cgggttcggc cggtcgagca cgacgaaccc
     4501 tttcttcgcc ttcgcgcacg cccgcatcgc gagcaccatc gtccacacga acgtgtagta
     4561 gcggctgccg acgtcctgga tgtcgaacac cagcgcgtcg acgttctcga gcatctccgg
     4621 cgtcggcgac agcgatgctt cgctgtgccc gtagagcgag tacaccggta ggcccgtgcg
     4681 cgcgtcggtc gccgcgtcga cgccgatcat gtcttgcgcg tcgccacgca cgccgtgctc
     4741 gggcccgaac agtgccgtga cctggaggtc tttgcgcccg gtgagcagat cgatcgcgtg
     4801 gcgcagctcg cgatcgacgc tcgtcgcgtt tacgatcagg ccgagacgcg tgaacggtgc
     4861 cagctcgcta ccctgagcag cgagccgcgc gagtccggtt tcgatcgcca cgggcgacta
     4921 ctcgaagatc ggaacgggct tgagcgacac cttgccgtcc ttggtcacga cgcggaagcg
     4981 cacggcgcgg cagttgtact gcgccttgag cttgccctcg ttgaccttga gcttcgcgat
     5041 gaagttctcg aacgagacgt catcgggcga gcccgcggcc ttgcgtgcag cgacgtactc
     5101 ggtgaacacg cgcttgtagt agtcgggctc gctctccttc gcgaggttcg cgagatccgg
     5161 atcgacggcc ggcttctcct cgccctcttc gaagtcgacg cgacccggga tgatcgggtt
     5221 gccctcttcg tcgaactctt cctcgccggg ctcgggacgg ccgagcaggc gcgcgagcat
     5281 gacgttgagg ccgttcgcga gaccgtcgag ctctgcaccg accgggcgga acgtgcgatc
     5341 gacgttgccg ttgatgatgt cggtgacgcc gagctcgatc tgatcgacct gcgcgacgag
     5401 ccggcgatgc gtgaggtaga gaccgagcat cgagatcgcg agcgcgccga gaccgagcag
     5461 gatgatgaac atcttgatcc cgcccgcgat gacactgtca ccggcgctcg cgagcaccat
     5521 cgcgcctgcg gtcaccgccg ggtagtcctg cggcatgcgg cgcgcgtttg tcgtcgagcg
     5581 cggcatctgc acggcggcgc cgtagtacgt cacgccgtcg atcgagacct tcgtgggctt
     5641 gtccgagccc tctttgatct tgccggagcg cacgagctcg gagagctgcg tcgccttcgc
     5701 ggtgtcctct tcgttcgtgc cgcccttcgt gaagctggtc gcgacgacgc gctgctgatc
     5761 gtagtacgcg atctcggtac cgagcagccg cttgtccatc tgtgcctgct gcgcggtctg
     5821 cgcgtacgcg acgatcacgg cgccgatgat cacgacgcca tccggatcgt tcgccggcac
     5881 cggcgcgtcg gcatcgatga tcggcgcgac gccgatcttc atcatgcggc cgcggtcgtt
     5941 ccagatgtcg ctgatgatga cgcggctgcc gagcacgacg ttgagcgcgg ggatcgtggt
     6001 ctcaccgggc ttcgcgcctt ccttctggaa ttgcttcggc acgacgctgg cgacgtcgtt
     6061 catcgcgacg agcaggccgt tcgcgtcgac gagcgcgatc atgtccggct tgacgtcgcc
     6121 ttccttctcg ttcgaggtga acttcgtcat gcccacgcgc gcttggttcg cgcgctccat
     6181 ggcgctctcg ctcttgagcg cgagcacgaa cgccttgtcg gcggcgagcc gctcggcctt
     6241 gttcgcgacg tcgatggcct cgagctgggc aagctgctgg acgacctgat aggagcgccg
     6301 cagctgggcc tccgcgtcct tgcggatctt gtcctcgaag ctcgttttcg taacgaaaaa
     6361 tgcgatcgct gtgagcgccg cgatcacgac tgcaactacg gcggcgataa ccgtgcggga
     6421 catgctccct ccgtcggcga ttattcgtgc gcttccaagg ggatgtcaat caacggcgga
     6481 tcacttcgcg gtgccgaacc gcttggtcat ctcgcagatc gtatcgaggc gcttcacgtc
     6541 gtcttcgttg ttcgagttca ggtacaaaaa ctgaaagtca cagaccgcac gcgccacgcc
     6601 cagccgcatg gtctgggcca acgtgccgcg gtcatctgca atcaacagct ggcgtttctc
     6661 gccgcgcagc gtggtcgtga agccatcgct ctccgtgcac ggctgcgtcg atgccggcgt
     6721 gattgccgcc cgcggctcgc aaagtgtcgc ctggggtagc gcgacgatct cctgaatcac
     6781 gtcgcggcgg gcctggacat cgggctcgaa ccctttgagc tgctcgagca cgagtcggta
     6841 cgcggcctcg acctcgcccg ccgagacctt gccgtcggtc tcgaccacca gcccatccgg
     6901 gaaggtcttg ccctcggtga gtcccttgat cttcgtcgcc gccttgaagc cggctggctt
     6961 gttgtcgccc atcagcagcg cggctgcggt ggcaccgccg ccgagcacga gcacggcaac
     7021 cgcgatgacc ggccacttgc tgccccgctt tttgcgggcc ggcaagccgg tggtgcccgg
     7081 ctcgagctcg tcggcgagat cgttcgcggt ggccgcgcgc acggtctcgg caaccgccac
     7141 tgccgccctc accggaggcc gctcgtcttt cgtcgcccgg acgtcgatca gatcgcgcga
     7201 gaaccgctgc gtctcgacga gcgcgacatc ggcctccttg agcgccccct cgagcacctt
     7261 gcgcatgtcg cgcgcactgt cgaagcgatc gggtgccttc ttcgcgaggc atcgcatcag
     7321 cgcgtcctcg accgcacgcg ggacctcgat gccgaccacg ctcggcggct tcgcgatctc
     7381 ggacaggtgc ttcgtcatga tctcgaagtg cgtgctgccg tcgaacggcg tgtcgccgac
     7441 gagcgactcg tacagcgttg cgccgagcga atagatgtcg gtgcggagat cgacctcctg
     7501 gccgcgcacc tgctcgggag acatgtagcg cacggtgccc atcgtctggc ccgtcgcggt
     7561 cagccgtgtc gactgcgcca tcttcgcgat gccgaagtcc atcaccgtcg ctgcaccgtc
     7621 gctctcgcgc acgagcacgt tcgacggctt catgtcgcga tgcacgacgc cgcggccgtg
     7681 cgcgtactcg agtgccttca gcacgtcgat cgtgatcttg cagctcgcct tccagtccag
     7741 acgcttgttc tcgaggatca agcgctccca ggtgcggccc tggacgaact gcatcgcgag
     7801 cacgaagctg ccgttctcct ggccgaagtt gtagaggtgg acgatgttcg ggtgatcgag
     7861 tgccgcgagg gctttcgcct cgtcgaggaa gcgcgacttc acctggctgt gcgcagcgag
     7921 ctcaggcggc aagatcttca gcgccacttc ctgatcggtc atcttgtggc gcgctttgaa
     7981 cacgacgctc atgccgccct ggccgagcac gtcgacgagc tcgtacgtgc cgaggatgag
     8041 cgagccgatc atccggtgcg cgccggttgc gccgagcgcg gtgccgcagt gcgggcactt
     8101 gacgtcgatg tcgaggacat cgttctggca gctcgggcac ttcatgccgt cgcctctgac
     8161 gcgcgacgct gccagcccgg aatccgatcg agcagtcggc cgagctcgtc gacgaggatc
     8221 gccggatact cgaccggcgt gtagtgcgta ccgccttcga tgaccaccat gcgcgagccc
     8281 gggatcgtgc gatgcatgtg ctccgcggtc gagggcggtg tcatcagatc gcgatcaccg
     8341 gtgacgatcg agatcggcac cttgatctgc gggagcacct cttcggcatc gtgctcgtcg
     8401 agtcgggtca acaagtcact gtagattcgc cagtcgatcg tctgaaagcc tgcggcgacc
     8461 gcgcggaaga tctcgatgtc gatcgtctgc gacacgaggc cgacgcgctt catcgcagcg
     8521 atcagcgcgt cgctcccggc gagccgcttc gtcgcgtagc cagcgagcgc cgcttgcgct
     8581 ttgaccaagc gcagcagcat cggaatcgtg cggccgacga gccgactgcc catcacggtg
     8641 cgaaacgcgc gcccgtaggt gccgttgatg gcgagcatgc cgcgcacgcg ctcggggtag
     8701 cggcggatcg cttcgaagcc gacctgcacc cccatcgacc agccgacgat gaccgcgctg
     8761 tcgaccttct cctgatcgag gatcgcgacc aggtcgtcga cctggtgcac gaccgtgttc
     8821 gcgttcggat ccttgctcgc gtctgacgtg tagagcccgc ggtagtccca gcacagcgtc
     8881 tggtaggcgt cgtccagcgc gctgtagagg tagcgaaacg cgatgtacgt gccgccgagg
     8941 ccgttcgcga acacgatgca cgggcgcgat ggggcgccgc gcacctgata accgattcgg
     9001 gtgccgtcgc gcgccaccac gtgacgctgc tggatccccg ggaccattcg cgagtgatcg
     9061 tagccgaaaa caaagacggc cggggaaacc ggccgccttc aaccagagga ctctcagaat
     9121 cagaagtaga ggatcgcgtt gagacgaccg cgcgtgtcct cctcggtctc gtcgctgctc
     9181 gcgctcggcg gcgtgatcac gcggccggtc gtgcctgccg gcgtcagcat cggctcgtcg
     9241 ctcgcgacgg tgccacggct gcccgcgcgg aactcgagcg cgaggtggaa gcgcggggtc
     9301 accgcccagc ggaggcccgc accgatttcg ccgtagtcct gcgtcgtcgt gaactcgcca
     9361 ccgacttccg cctgctgcac gccggtgccg acgagaatgt acggcgccca gcggttgtac
     9421 gcgccgattt cgtagagcag cgatgcaccg aggcgacgat cgacacgcgc gttctcgacg
     9481 ccgtcgacgt cgtacgtcat cttgccgatc tcgccttcga tgatgaggcc cggcgtcaag
     9541 cggaagcggc ccaggaggcc gaggtcatcc gactcgtgcg tcgagtcacc ggtctgcgac
     9601 tcgaccgccg agccaccggc gaagaggccg ataccgaggc ggggcagcgg ctcgcgcacc
     9661 ggggtcacgg cgacgacgcc aggcgcctgc acggcagccg gttgaaccgg gtagtacgac
     9721 gtcgactcgt aatacgacgg gacgtacgcc gggtagtaaa cgtagtaagg ccgcgggtag
     9781 tagcgatacg tcggcccgac gtagatcgaa ccgcggacgt gccaccgcga ggggctccac
     9841 gccgggcggg cgaagtgcca cgagccggaa gtcacgctca cgccgccgct ccagcggacg
     9901 ccgccgctaa agcgaacacg ctgcgcctcg gcaacgccgg aaacgccaag cgacgaagcg
     9961 acgagagcac caaggacgag agggaggcga acgttcactg ggttgaccct ttctaggaac
    10021 acaagacgct cgaagcgccc gaacggttta gtgcatgcac agggccagct acgacattgc
    10081 cggatcccga ggacaaacgg gtaaagatcc gctcgcttac atgcccgaga agagatcctg
    10141 ggtgtgacca cttattcacg acttgcggtt gtgtggcgat gcgaagacat cccgagtgca
    10201 acgggtccaa gcctcgggca tccttgattt ctcgacggca gctccataca gtcaccctcc
    10261 ggaggagaac gacattgagc gacgcaaaga aagcgggcag tagagacatc agcgagctca
    10321 agcagcgcct tggactcaag aaaggcggag cggcgcagcc tgctgccggt ggtcgttcga
    10381 acggtgctcc atccggtggt gtagtcgcac cgccaggtct tgccgttccg caagcgccgc
    10441 cgcagccggc cgccccgaac gcccacgacg atccgttcgg tgccatgaat cacatggccg
    10501 ccgttgcgca agtccagcgc gcgcccgaga aggaatacat cgtcgtcaac gacggcaatg
    10561 tcgagaaagt tcatgccgcg tcgcacggcg cgttgatcgc gaagattgca gtgccggctg
    10621 gtattgctct catcgtcggt atcgccatcg gcaagatcgg caccggcgcg agcaactaca
    10681 acgaaggcct ccgcggcgcg cgcgtcgtcc tcggcgacaa gacgtcgggt tcgacggtcg
    10741 cgatgctgaa gaagcagctc tcggatctcg ataacctcct cgacgaagcg aagacgaaga
    10801 acggtttcaa gccgagcctc gagctcgaca agaagctcaa ggaagcggcc gccaagctcg
    10861 aggtcaagac cgaggtcgtg ttccgcgcga agcagatcga tccagagctg tccggacagg
    10921 tgctctcgtt ctacgccggc gtcctcgaag tcaaagacat gatcgacatc cacaacaagg
    10981 ctgctgcggg tgacgacatc atgctgaaga aggccaaaga cgctgccgac aaggcggcga
    11041 ccgacgatgt ccccggcggc ctgcgcttcg cggttctgct gtcggcgccg acggaaaccg
    11101 atcgcaacga attcggcgcg aagctcgtcg agatcgccgg cgtgtactgc ggcgccggaa
    11161 acaacccggt ctcgcgctgc cccgagggcg agttcccgtc ggcatacgcg tatcgcaacg
    11221 agccgggcgc cacgccgatc aagggcgacc tcgtcccgac cggcagcgac acgctgcccg
    11281 gcaagaagat cgttccgctg ctctcgaacg gcgtgcgcga ctcgttcatc aagggcggcg
    11341 agccgacgat ctcggagatc tactacacca agcgtttgcg tgcgttgtac gaccgcattc
    11401 acggcaaggt cggccaggac ggcaagcccc agggcggcct catcgaggac ggtaacaagc
    11461 tggaaacccg tctaacgacg gaagccggca agagcacccg gttctcgttc ttcatgtgat
    11521 gaatcttctc gggacctgaa acgtcggagg acgtatgcgc cggatccgca ctgcactcgt
    11581 aattgccacc ctcgtcggtt ttggtcttca gattccggcc gcgctcgcgg ctgacgcgaa
    11641 gaaagccgcg cccgccaaga aggcgccggc gaagtccgcg aaggcgaaag ccacgccgcc
    11701 gaagaagaaa gagccgccgc cgatggtgaa cgccgagcac aagaaggcgc tcgcggaaaa
    11761 gttcgccggc ttcaagttcg gcatgacgaa ggacgaggtc ctcgccgtgc tgcagaagca
    11821 gatcaacgag cgcttcgaag agaaaatcaa agcgacgacc gacgtcgcga tgcaggatcg
    11881 ctatcgcaag gacaagaagg ccgagctcgc gcgtacgtcg agcacgtacg tcgagttcaa
    11941 cggcaagaag ggcggctggg acgtctcgat cgtcgagaac gagttcgccc acaacaccgg
    12001 cgagtcgatg atggagcgct gggaaaacga cggcggcaag aaccagcgtc gcttcttctt
    12061 cttctacaac cagcggctgt ggaagatgtt catctcgctc gacgtgtcga tgattcccga
    12121 cgacaagcgg aacttcgaca cgttcaaggg cgtgatggaa ggccagtacg gtaagggtgg
    12181 cgtcgacggc ggcacgatcg cgtggcgcgc cggtgagttc gatgttcgcg cgatcgatcg
    12241 cctgaaggac tacggcgcgc tcggtctcgt gctcgaggac ccgaaggttc gcagcgaagt
    12301 cgtcgcgctc cgcgagagca aggcgccgcc gaagcaggag acgccgtcgg tgatcaaggc
    12361 catcatcgat cccgacaaca aggatcatcc cgacgtgaag tcgaacagcg gcgcagtcga
    12421 ggccgtgatc cagggcaacg gcgccgcaac gcctccgaag aaataggcga caccctcgcc
    12481 taccgacgag cccgctccgt gcgggctctt tcgttttcgg ttagcttcgc cgccgatgaa
    12541 acggcgaacg ttccttcgcg gttccgcggc gggcatcgcg atcgcgggcg cgcattggct
    12601 ccgcgtcccg acgctcgtgc gtgccgacga caagcccgcg gccgcaggtg acagcgcagg
    12661 ccagctctac gagcttacgt cgcggcccgc gaactacgag tcgacgcgcg acacgtttac
    12721 gacgcgcatt acgcccgtcg agcggttcta cattcgcaac cacttcgaca ttccggtcgg
    12781 cgacatgaag gactggaagc tcgagctgaa ggggctcgtc gccaagccgc agaccttcac
    12841 gctcgccgac ctcgagaaga tgaagcagac gacggtcgag gcggtgctgc aatgcgcggg
    12901 caatggccgc ggctggttcg tgccgcgcgt gcccggcgtg caatggcggt ggggcgcgat
    12961 gggcaacgcc gaatggaccg gcgtgcggct gaaggatctg ctcgccgccg ccggcgtcgc
    13021 gaaggaagcg cagcatgtcc agctgcaggg cgccgagaag ccgacgctcg agaagacgcc
    13081 agcattcatc cgcggcatcc cgatcgcgaa ggcaatgcat cccgacacgc tcgtcgcgct
    13141 gcgcatgaac ggccagccgc tgtctccgca tcacggctat cccgcgcggc tcgtcgtgcc
    13201 cggctgggtc ggcgacgatt ggatgaagtg gctggcgacg atcgaggtgc gcgccgacga
    13261 gccgaagggc ttcttctacg agaccggcta ccgatttcca gcgacgcccg gcaagcccgg
    13321 cgaaccggtg ccgcccgatc agatgaaacc gatgacgaag ctcgtcgtga agtcgcagct
    13381 cggctcgatc ggagacggcg acgtgatcgc gccggggccg cacgagctgg tcggcgtcgc
    13441 attctcgggt gaggcgaaga tcaagaaggt cgacatcacc gtcgacggcg gcgcgacctg
    13501 gcagccggcg aagctcgacg cgcatgactc gggctacggc ttccggttgt tccgctacac
    13561 gtgggacgcg aagccgggca agacgaaggt cggcgcgcgc gcgaccgaca gcgcaggcgc
    13621 ggtgcagcct gcgactgccg tgtggaatcc agcgggctat ctctacaacg cgatcgagat
    13681 ggtcgaggtg gaggtgcgcg cgtgagtcgc ttcgtcatca tcgtgctcgc gctcgctgcg
    13741 tgtcgctccg aagacaagcc cgcgcccgca caacaaccag tgccgacgca accgacgccg
    13801 cccgcggctc cgccggtgac gatcgatcgc gatgcgctgc tcgccggcaa gctgcccgag
    13861 ggcgcgccgg agaccgacct cgtcaacgcg caatgccgca tctgccacag cgtcgagtac
    13921 ctgacgcaac agcgtctcgg cgaagccgcg tggaagaaga cgatcgacaa gatgcgaaag
    13981 ttcggcgcgg tgctcaccga cgagcaagcg gcgtcgctcg tcgcattcgc cgcgcactac
    14041 tggaaccctg atcttccggg acgtacgtgg actccggggc cgcctccccc aggtgccctt
    14101 ccgatcacga cgaagtaacg cgagaagttg cgttcacgcg cgcaatcgcc gcgctggcga
    14161 ttcgagttgc cgtgacagct cggcgcgacc gtcgcaaaac gcgtcgcgct agcacgtgct
    14221 gctcggcggt ttcggtgctc gatcgcggca cgggagctgc tctgtgctac ccgcctcatg
    14281 aagacgttga actggttgtt ggctgcggga ctgatgacac ttgtgggcgc ttgcgccgaa
    14341 ggtggcgacg acgatctctc ggatgcaagc gagctgtcgc tgtcggcgaa cgcgttctcg
    14401 cacacgaaga tcgtcggcga cgtcgatctg ggcttttcgc gctcggtcct ctacaagagc
    14461 tcgccgcgct atcgcgccgt gaggttccac gcggaggcgg gcacgatgct cgaggtgtac
    14521 gtgcgctcga cgcacggtga tccgacgacg tggctgactg acgtgaactt caaggtcatc
    14581 gcgcataacg acgacgcgaa cgaagagacg agctccaaca tctcgatcga gagcctcgcg
    14641 aagaccggcg actactacgt cgtgttccgc gacaagcact acgagagcca ttacttcgac
    14701 gtcgcgcccg tgcagctcga tctgcccgcg catcacccga tggtcccgga cgtcgagaca
    14761 atctacgaag ctctcgtcgc gtcgcacacg ctggcgacga agcagatcag cggcgacggt
    14821 ctgccgtttc tcgcgaaggg cctctacgcg cggttcacga gcgaagcggc agccgaaggc
    14881 gcgcaagccg ccgcgtacgc catcgaggtc gacggcgaga ccgtgtggct cgtccgcaag
    14941 tacaaggcgg gcgagtacct ggtggtcggt gcgtacgtcg agagcggccc gatgctcggc
    15001 atcgccggcg gcacctccga gttgatcgac aactgggagt actgatgcgg taagaacgcc
    15061 gcgtggacga gatcgaagca gagctcacgg cgttcggcga tccggctcgc aacatccgag
    15121 acgacgcgtt cgaagcactg cacgaccgcg tgttcccacg cggcacgcat ggtgagcttg
    15181 cctggacggt cggcgtgcga atcgccccgc gcgtgatcga gctggcgacg cggcccgaag
    15241 gcgcgtgggc ggcgatcctg ctgctggatc tggtcgcagc cgcccggcgt ggcggccacc
    15301 gcaaggtgct cgatgccgcg gcaccactcg cgattgccga tttcagcgcg gtcgcacctg
    15361 cggtccgtgc ggcgctcgag ctcgcacggc agctcgcgat cggtggaccg atgccatccg
    15421 acgaggtgat cacgaacgcg gtcgacgatg cggtgccgga cgagaacatc gcggcgctgc
    15481 ccgagcacca gaagtaacag cgccttcgcc ccgggatcgg caccccggcc gcggtgtcgg
    15541 agggccgtgg tacggctcct cggtggctcg gtcgcctgca gagacgccgc tcatgcggca
    15601 gtacctcgat ctaaaggaga agcatcccga cgccctcctg ttctttcggt gcggcgactt
    15661 ctacgagatg ttcttcgaag atgcggtgat cgcagcgcgt gcgctcgatc tcgtgctgac
    15721 ctcgcgggac aagggcaaag aagattcggt gccgatgtgc ggcgtgccct atcacgcggc
    15781 gcgcggctac ctcgccaagc tcaccgagct cggccacaag gttgcggtat gcgagcagat
    15841 ggaggatccg aagctcgcca aggggttggt caagcgcgag atcgtccgcg tgatcacgcc
    15901 cggcgtggtg ctcgacgacg acgtgctcga tccgaagctg ccgcgctatc tcgcggcgct
    15961 cgtaccgggc gatcgcggaa agagcaccgt caaggcgcgc cccgacgagg tgctagtcgg
    16021 gctcgcgtat ctcgacgcga cgaccggtga cttgttcgcg accgagattg cgatgtcggc
    16081 ggtgctcgac gagctgatcc gtgtgtcgcc gcgcgagctg ctcgcggatc cgagcgtgct
    16141 cggtgcggag cgcaatccgc ttgcggtgct gcagcagcgc ta
//
LOCUS       DASUFX010000594        12468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1902302, whole genome shotgun sequence.
ACCESSION   DASUFX010000594 DASUFX010000000
VERSION     DASUFX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902302"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFV99_23775"
     CDS             complement(<1..529)
                     /locus_tag="VFV99_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062420.1"
                     /translation="MSRRIIWIALAGACGSSKPGPSTTPASSGPITHETVSEPTPDTP
                     TAPSGEPAIPGSGNRCDMLPTGDLSIDGLLDDWKDKHLLARAGSPADGAMDVRCVWDG
                     KAIAFAIDVKDDRLIRVKGGQEDHVTLALAAGGRPVSVDVYPGNSMAKPKIAKPARVE
                     AADSLQPKGFSVEVLI"
     gene            601..1119
                     /locus_tag="VFV99_23780"
     CDS             601..1119
                     /locus_tag="VFV99_23780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062421.1"
                     /translation="MRGRAQVAPTAMRKISLQRLLSKLAVIALATSSPALASPNDQVK
                     PDAELVAGGFIDAGTVDTRDAAALLGRGLATALDGMKPLDATHLASSWATSEDPMRRL
                     GIARALEWSFPLVGDAIVIEHLSHDPDPHIRAACARAAWIRRGIGGDLGVLDRLSRDP
                     DPMVAAIALWPR"
     gene            1192..2904
                     /gene="ffh"
                     /locus_tag="VFV99_23785"
     CDS             1192..2904
                     /gene="ffh"
                     /locus_tag="VFV99_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5062422.1"
                     /translation="MLETLAKGFRAARQRLTGVAELNDEIIDEALRDVRLSLLEGDVE
                     FKVVKRFLERVKEQARGKQIELRAKSQEYGTKTITPEQAFIAICQDELIKMMGPVDTE
                     LNWSKGKTPTGIMMCGLQGSGKTTTTGKIARWLEKTHKKKPMLVAADIYRPAAVEQLK
                     TLGRQLSMPVYSAEGKDPVTICKEGMDAAMQQGCDAILFDTAGRLSIDEPLMQELEDI
                     NKTVKPGNIFLVLDAMTGQEAVNVADTFNKRLDVDGIIMTKLDGDARGGAALSVKEIT
                     GKPIKFLGMGESLDKLEEFRPEGLASRILGMGDIVGLVKDFEQVVDAEKAEEDALRML
                     KGKFDMQDFLEQIRLIQKMGSLKDLFEKLPFFGGGLPEGVNLDDRELTKIEAMISSMT
                     YEERMNPQVFVATSWEDYSTTAGRAAKRRRADFHPGRVNRISKGSGRSENEVKELLQK
                     FAQMRQMMVQLGASTGLMGKIPGFKQFSQMKKLANMDINALMGAGGLPPELAGMASGG
                     GGMPNMPSMPGVPSGHVPGLPKGFTPPGTKSLVGGGNNSARDRQKARDKRKAEKAARK
                     KNRR"
     gene            3007..4002
                     /locus_tag="VFV99_23790"
     CDS             3007..4002
                     /locus_tag="VFV99_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062423.1"
                     /translation="MTPDIVKLRDSLPTLEPILDRLELELVDDPTWKEPSDTSYLTDA
                     DRNNPDIAANVDAMTETNKLIAWFGRDAEGYLGLWRGPNNRSLTEAPVVRLDSEGQYS
                     IVAATVPDFIAIAMPEDEFANTRDTLTKAGFKVGFNPDAIWGALDAFDDDPNAYRNEL
                     YEQNRVARGLPRTDEEPEAEDTIVGDGVPEPRDFASGSMSSFPAIAVPDTAAADDFDD
                     DDDDGGGEVAGDLDSDDDLDDDDEEDYAPPPNAKQAVMKPAPAKAAPKAPAKAAAKAP
                     AKKAAAKPAKKAAAKPAKKAAAKAPAKKAAAKAPAKKAAKPAAKKPAKPAKKKKK"
     gene            4123..5424
                     /locus_tag="VFV99_23795"
     CDS             4123..5424
                     /locus_tag="VFV99_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062424.1"
                     /translation="MFLRCTVVLLVVAACGGSPASMMEPAPDGDGDGSSSIDATPLPA
                     DPCGGMTAACPTVPAGFGRGDGLRAIDHCAFPMTDRATWDDRGALIDALPQALTRVPL
                     ADIATDLNRTGVAQTAAQVPGNPPGVTRAFAWQSGDESVTYWIPQGIAGSFEARDDGV
                     VAGKKLVLVSWYYDIANDAGSTTEKGVRIAIVDATNAGAPTYRFALLVEPVARDGRTD
                     FAPVKVHAGGLAWYGDLLYVPVTGSGFRVFDLSRILRIDGTEDRLGYNATTGTYNAHG
                     YRYAIPQIGEYIDAGACNMVFSYVALDRTSTPHSLISGEYDATTVNGRVFRWPLDPST
                     GRLQLTDAQRVVPDGVWFAGESHVQGGLAHDNTFWLSSSQPAGSAGALYRARVNMPST
                     TLGWIDSPEDLAFDPKLDAVWSLSEATNARSVIAVRLDAID"
     gene            5466..7058
                     /locus_tag="VFV99_23800"
     CDS             5466..7058
                     /locus_tag="VFV99_23800"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5062425.1"
                     /translation="MARAANFDLATELGKPSFTPSQRDAPALVELVVAGEDPTAERAA
                     TALTVLGDGGRRAIADRLAGDDRHPDEDATELGDGATARLVSALGLFARRGDAEARAA
                     LLSRMHDRVARVRRAAIVALGKLEGDDARTALVARWDAGDVTPDERRALAEALGKIGG
                     DEALARLRALDPGDDAELARRRDRALLIADRSAKRGEDSRVAIDVDPPAPLVVRLRCK
                     PGLGSLLVEDLAARGFAPTPHGDAAADVTLARAWSALFASRLWERAAIRVPLRSDEPR
                     AIVETLTAPRIRALLQAWTVGPIRWRLGFAQGHKRAIVWQVAKDVTARAPELVNDPTQ
                     TTWDIAVTDDALEVSPRRLDDPRFAWRIADIPAASHPTVAAAIAYVGEARHGDRVWDP
                     FVGSAAELIERTRLGTYASLLGSDLDDNALAAARNNLAAANVDAKLVNADARTYDPGD
                     IDLIVTNPPLGSRVQVDAAALLVACLPNFARALAPGGRLVWITPAQKKTTKVAEQLGL
                     RRTRSLAVDLGGVRGHLERWER"
     gene            7068..7754
                     /locus_tag="VFV99_23805"
     CDS             7068..7754
                     /locus_tag="VFV99_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062426.1"
                     /translation="MPVAVRTWLRYVVPLTLLAALAFLPLLYVASRSGAAADLVKARA
                     QVRLGWILAGFAWMSIYLLVAGVAPAVRSVAAGAPLSQWRALADGARNLVRAFVPWLI
                     VVFAVALGEVALVIPGFLIGILLSLTGASVRIGEPPPAALVDSVAVVRRRFGHVALIV
                     IAILLANLAICYALQATFVPHITRKVAATKLLPIRTFVRLVPVAIAALSPLAACVLAA
                     TYGRLTRRTS"
     gene            complement(7735..8421)
                     /locus_tag="VFV99_23810"
     CDS             complement(7735..8421)
                     /locus_tag="VFV99_23810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5062427.1"
                     /translation="MPKSVVTFDIGQTLVDLDLDFLALRLGQRGVTVPTDALRAALPT
                     AWQRYDALTESGASHPWHELMQSLLTGAGVADPMPHVAWLYEQQATANLWRKPIPAMV
                     QIVRELRARTNDVALAALSNSEGHLAELLAEIELAPLFDTIIDSGRVGVAKPDPRIFA
                     LTLEALAVEPTVIVHVGDSWAADVEGALAAGWNAIWYRSRASKGNADPRVPIATNAAE
                     TRAALSSFGV"
     gene            8471..9556
                     /locus_tag="VFV99_23815"
     CDS             8471..9556
                     /locus_tag="VFV99_23815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5062428.1"
                     /translation="MPEGPSAQQALVPGVLVVWSGAQPTVQAFRVPASGLVIGRELLQ
                     TTTDDRISRQHARIMWKDRRFVVTDLGSRNGTYAGGHALIDREVTVTPPSVVRTGRTV
                     SVLLEDIRRFEGGAMETKFDAIVGASTRHLWTQMEQVAKANQNLLIIGEPGVGKGRMA
                     RGYARVRTKPEAVFNPTIQAVPLERIAAPQVETLILEQVGKFGAQNTQTLARLLETRP
                     NLRIVTTAVMPLEHLGLPAEIAVKLSSATLHMPPLRDRPDEMAFLVQDAVRASEPGLQ
                     IHSTLIEACLLRPWPGNSRELVGEVGRTAHTVASQGKNNIRGEDLDNDAGHLMVGAPT
                     LNAAVTPTAVGKQRRKRASSRPNSRNE"
     gene            complement(9767..10759)
                     /locus_tag="VFV99_23820"
     CDS             complement(9767..10759)
                     /locus_tag="VFV99_23820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5062429.1"
                     /translation="MAEPDALRVVEAGYRWVEDEREWLTGVAEAARAFAIGTGIAAYT
                     VELGDAPAIQSYVATKVADDFETRVRAFTSSFDAATARLMYAPTEFVGNSQFRLRRIA
                     RMRKKSVAKLTRGTAVSAFALIGGDPRSKSVALVFPATSDDVDPEQPFPRARMLGLAA
                     AHLGAATRLRQLATPSAIDGDGTESILDANGKVLHAIGDARDKAQRESLVEAVVRRER
                     ARGRLRRTDPEEAAQLWSVLVSGRWSIIDFVDRDGKRLMLARRNPVNGPDVLALDEQE
                     RDVVWLATLGHSRKYIAYELGLATATVTKRLGNALKKLRIRNRRELLRKLGRAA"
     gene            complement(10752..11042)
                     /locus_tag="VFV99_23825"
     CDS             complement(10752..11042)
                     /locus_tag="VFV99_23825"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5062430.1"
                     /translation="MPRGKRIDPPPNLVAEKLDDELVIFSWDVSAPPPMLTSAERDVL
                     ARVVRGESNAAIARARKTSVRTVANQVASLLRKTGAASRFDLIRRYGGLRDG"
     gene            complement(11108..12436)
                     /locus_tag="VFV99_23830"
     CDS             complement(11108..12436)
                     /locus_tag="VFV99_23830"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX5062431.1"
                     /translation="MKRAIAIAFMVGCAAEGQPDSTDVSVLEQDATVCGVGPTVKGID
                     VSYYQGTINWSQVAGDGVKFAFIRVSDGTGFIDPKFESYWSASRAAGIKHGAYQFFRP
                     GQDPLAQADLLLTKIGNKLAADDLPPVVDVEASDGLTAAQIQAKLKVWVDHVKAALGR
                     DPIIYTGFYFWRDSVGAPDYADTPLWHAQYTTAACPNIAPPWTKWALWQYTSTGTVAG
                     ISGDVDVDRWNGDLASLQAFLGPPEPVLPCQSIGYAGAVIDDGDSCFEPGGPVASMRA
                     VSDAGQDGDLVWTHTTEDAAEANFGLWHFDFEQAGRYKLEVYTDAKYAQSKQAKYIVQ
                     ASGAMQDKVIDQSAADGWQTLGEFDFAQGGDQSLRLGDNTGEPLANNVQLVFDAVRLT
                     RIETETGSGSGSDDTDMMPGDGGGCSSGGGHMGFALIAVLGILRRRRSNK"
BASE COUNT         2099 a         4215 c         4194 g         1960 t
ORIGIN      
        1 ggatcaacac ctcgacggag aagcctttgg gctggagcga gtctgctgcc tcgacgcgcg
       61 ccggctttgc gatcttcggc ttcgccatcg agttgccggg gtagacgtcg acgctcaccg
      121 ggcgaccgcc tgcggcgagc gccagggtca cgtgatcttc ctgaccgcct ttgacgcgga
      181 tcaaccgatc gtctttgacg tcgatcgcga acgcgatcgc tttgccgtcc catacgcagc
      241 gcacgtccat ggcgccatcg gcaggcgagc ccgcacgcgc gagcagatgt ttgtctttcc
      301 agtcgtcgag caggccgtcg atcgacagat cgcccgtcgg cagcatgtcg cagcgattgc
      361 cgctgcccgg gatggcgggt tcgccgctgg gcgccgtcgg tgtgtcgggc gtgggctcgc
      421 tcacggtctc gtgcgtgatc ggtcccgacg aggccggtgt cgtcgacgga cccggcttgc
      481 tcgagccgca cgcgcctgcc aatgcgatcc agatgatccg tcgcgacatg tggaacatcg
      541 ggccccgaag ataccgaagg tccgccgcaa acggtggggc ctcctgcctc acttgacggg
      601 ttgcgcgggc gtgcgcaggt tgcgcccacc gctatgcgca aaatctcgtt gcagcgtttg
      661 ctgtccaagc tggcggtgat cgcgctggcc acctcgtcgc ccgcgctcgc ctccccgaac
      721 gaccaggtaa aacctgacgc ggagctcgtc gcgggcgggt tcatcgacgc cggtaccgtc
      781 gacacccgcg acgcggcggc gctgctcggc cgcggtctgg cgactgcgct cgacggcatg
      841 aagccgctcg atgccacgca cctggcgtcg agctgggcga cgtcggaaga tccgatgcgc
      901 cggctcggta tcgcgcgcgc gctcgagtgg tcgttcccgc tcgtcggtga cgcgatcgtg
      961 atcgagcacc tctcgcacga tccggatccg cacatccgcg cagcctgcgc acgagcagcc
     1021 tggatacgcc gaggaatcgg cggtgatctg ggcgtactcg accggctgtc ccgcgacccc
     1081 gaccccatgg ttgcggccat cgcgctatgg ccccgatgat cgggcacttc gccctgggtt
     1141 ctcgtcgctt cccgcagggt tgaaccagcc tccgtatggt ataggacgac ggtgctagag
     1201 accctcgcca agggattccg ggcggcccgg cagcgcctca ccggcgtcgc cgagctcaac
     1261 gacgaaatca tcgacgaagc gctgcgtgac gtccgcctct cgttgctcga aggcgacgtc
     1321 gaattcaagg tcgtcaagcg cttcctcgag cgcgtgaagg agcaggcccg cggcaagcag
     1381 atcgagctgc gcgcgaagag ccaggagtac ggcaccaaga caatcacgcc ggagcaggcg
     1441 ttcatcgcga tctgccagga cgagctcatc aagatgatgg gccccgtcga caccgagctg
     1501 aactggtcga agggcaagac gccgaccggc atcatgatgt gcggtctgca gggctcgggt
     1561 aagacgacca cgaccggcaa gatcgcccgc tggctcgaga agacgcacaa gaagaagccg
     1621 atgctcgtcg ccgcggacat ctaccgtccc gccgccgtcg agcagctgaa gacgctaggc
     1681 cgccagctgt cgatgccggt gtactcggcc gagggcaagg atccggtcac gatctgcaag
     1741 gaaggcatgg acgcggcgat gcagcagggc tgcgacgcga tcctgttcga caccgccggt
     1801 cgtctgtcga tcgacgagcc cctgatgcag gagctcgaag acatcaacaa gaccgtcaag
     1861 ccggggaaca tcttcctcgt cctcgacgcg atgaccggtc aggaagcggt gaacgtcgcc
     1921 gacacgttca acaagcggct cgacgtcgac ggcatcatca tgacgaagct cgacggcgat
     1981 gcccgcggtg gcgcggcgct gtcggtcaag gagatcacgg gcaagccgat caagttcctc
     2041 ggcatgggtg aatcgctcga caagctcgag gagttccgcc ccgaaggtct cgcgtcgcgc
     2101 atccttggca tgggcgacat cgtcggcctg gtcaaagact tcgagcaggt cgtcgatgcg
     2161 gagaaagcgg aagaagacgc gctccgcatg ctcaagggca agttcgacat gcaggacttc
     2221 ctcgagcaga tccggctcat ccaaaagatg ggctcgctga aggatctgtt cgagaagctg
     2281 ccgttcttcg gcggcggcct gcccgaaggc gtcaatctcg acgatcgcga gctcaccaaa
     2341 atcgaggcga tgatctcgtc gatgacctac gaagagcgca tgaacccgca ggtgttcgtc
     2401 gcgacgtcgt gggaggacta ctcgacgacc gcgggccgcg ctgcgaagcg ccgtcgcgcc
     2461 gacttccatc ccggccgcgt caaccgcatc tcgaagggct cgggccgcag cgagaacgag
     2521 gtcaaagagc tgctgcagaa gttcgcgcag atgcgccaga tgatggtgca gctcggcgca
     2581 tcgaccggcc tcatgggcaa gattcccggc ttcaagcagt tctcccagat gaagaagctg
     2641 gcgaacatgg acatcaacgc gctgatgggc gccggcggat tgccgccgga gctggcaggc
     2701 atggcgagcg gcggcggcgg gatgccgaac atgccgtcga tgccgggcgt gccaagcggt
     2761 cacgtgccgg gcctgccaaa gggcttcacg ccgccgggca cgaagagcct cgtcggcggt
     2821 ggcaataact ccgcacgcga ccgccagaag gcgcgcgaca agcgcaaggc cgagaaggcc
     2881 gcccgcaaga aaaaccgccg ttagaccgcg ccaggcgcgc ggtttcggcc aatccggatc
     2941 gggttcacgc gcttcgcgcg tcgccggtat ccaacgcgtc tgcgaagcgt ggctaagatg
     3001 ccgggcatga cgcccgacat cgtgaagttg cgtgactcac tgcccacgct cgaaccgatt
     3061 ctcgatcgtc tcgagctcga gctcgtcgat gaccccacgt ggaaggagcc atccgatacg
     3121 agctacctga cggacgccga tcgcaacaac ccggacatcg cggcgaacgt cgacgcgatg
     3181 acggagacga acaagctcat tgcctggttc ggtcgcgacg ccgaagggta tctcggcctg
     3241 tggcgcggcc cgaacaatcg ctcgctcacc gaagcaccgg tcgtgcgtct cgactccgaa
     3301 ggccagtatt cgatcgtcgc cgcgacggtg cccgacttca tcgcgatcgc gatgcccgag
     3361 gacgagttcg cgaacacgcg cgacacgctg acgaaggcgg gcttcaaggt cggcttcaac
     3421 cccgacgcga tctggggcgc gctcgacgcg ttcgatgacg acccgaacgc atatcgcaac
     3481 gagctctacg agcagaaccg cgtcgcgcgt ggcctgccgc gcaccgacga ggaacccgaa
     3541 gcagaagaca cgatcgtcgg cgacggcgtg cccgagccgc gcgacttcgc ctctggatcg
     3601 atgtcgtcgt ttccggcaat cgcagttcct gacacggccg ccgccgacga cttcgacgac
     3661 gatgatgacg atggtggcgg cgaggtcgct ggcgacctcg acagcgacga cgatctcgac
     3721 gacgacgacg aagaggacta cgcgccgcct ccgaacgcga agcaagccgt gatgaagccc
     3781 gcgccggcaa aggccgctcc gaaggcaccc gcgaaggccg ctgcgaaggc accggcgaag
     3841 aaggccgccg cgaagcccgc aaagaaggcc gccgcgaagc ccgcaaagaa agccgctgcg
     3901 aaggcgcccg cgaagaaggc cgctgcgaaa gcgcccgcga agaaggccgc gaagccagcc
     3961 gcgaagaaac cggcgaagcc ggccaagaag aaaaagaagt agcgtcggcg tcgcggctcg
     4021 cgatgtgcaa cctcccgttg gcagcggcgt ggcggatggc gcctcgctga tgttctgcgc
     4081 gggcttagcc gtcggcccga cacttgctgg atgatggcca cgatgtttct gcgctgtacc
     4141 gtggtcctgc ttgtcgtcgc tgcctgtggc ggttcgccag cgtcgatgat ggagccagcg
     4201 ccggatggcg atggcgacgg ttcgtcttcg atcgatgcca cgccgttgcc agccgatcca
     4261 tgtggcggca tgaccgcagc ctgcccgacc gtgcccgccg ggtttgggcg cggcgatggg
     4321 ctgcgcgcga tcgatcactg cgcgtttccg atgacagacc gcgcgacgtg ggacgatcgc
     4381 ggcgcgctga tcgatgcgtt gccacaggcg ctcacgcgcg tgccgctcgc agacatcgcg
     4441 accgatctca atcgaaccgg cgtcgcgcag acggcagcgc aggttcccgg caacccgcca
     4501 ggtgtcacgc gcgcgttcgc gtggcagtcg ggcgacgaga gcgtcacgta ctggatcccg
     4561 caaggcatcg cgggcagctt cgaggcacgt gacgatggcg tcgtcgccgg caagaagctc
     4621 gtgctcgtca gctggtatta cgacatcgcg aacgatgccg gctcgacgac ggaaaagggc
     4681 gtgcgcatcg cgatcgtcga cgcgacgaac gctggcgcac cgacgtatcg attcgcgctg
     4741 ctcgtcgagc ctgtcgcgcg cgatggccgc accgacttcg cgccggtgaa ggtccacgcc
     4801 ggcggactcg cctggtacgg cgacctcttg tacgtgccag ttaccggaag tgggtttcgc
     4861 gtgttcgatc tgtcgcgcat cttgcgcatc gacggcaccg aagatcgcct cggctacaac
     4921 gcgaccaccg gcacgtacaa cgcacacggc taccgctacg cgattccgca gatcggcgag
     4981 tacatcgacg ccggcgcctg caacatggtg ttctcgtacg tcgcgctcga tcggacgtcg
     5041 acgccgcatt cgttgatctc cggcgagtac gacgcgacga cggtcaacgg ccgcgtgttc
     5101 cgctggccgc tcgatccgag caccggtcgg ctgcaattga ccgatgcaca gcgcgtcgtg
     5161 ccagacggcg tgtggttcgc gggcgagagc cacgtccagg gcggcctcgc gcacgacaat
     5221 acattctggc tatcgtcgag ccagccggca gggtcggcag gcgcgctcta tcgcgctcgc
     5281 gtcaacatgc cgagcacgac tctcggctgg atcgactccc cggaagacct cgcgttcgat
     5341 ccgaagctgg acgccgtgtg gtcgctcagc gaagcaacga acgctcggtc ggtgatcgcg
     5401 gtgcgtctcg acgcgatcga ttgacagcgc gccggcgcgc gagctgctat ggtccgcgcg
     5461 cagcgatggc gcgtgccgcg aacttcgatc tcgccaccga gctcggcaag ccgagcttca
     5521 cgccgagcca acgcgatgcg cccgcgttgg tcgagctcgt cgtcgccggc gaagatccga
     5581 cggccgagcg tgcggcgacg gcgcttaccg tgctcgggga tggcggccgg cgcgcgatcg
     5641 cggatcgact cgcgggcgac gatcggcatc ccgacgaaga tgccaccgag ctcggcgacg
     5701 gcgcgaccgc acgcctggtc agcgcgctcg gactgttcgc gcggcgcggt gatgccgaag
     5761 cacgcgcggc gctgctctcg cgcatgcacg atcgcgtggc gcgggtgcgg cgtgcggcga
     5821 tcgtcgcgct cggcaagctc gagggagacg atgctcgcac cgctctcgtc gcgcgctggg
     5881 atgcgggcga cgtcacgccg gacgaacgac gcgcgctcgc cgaggcactc ggcaagatcg
     5941 gcggtgacga agcgctcgcc cgcctgcgcg cgctcgatcc aggcgacgat gccgagctcg
     6001 cgcggcgccg cgatcgcgca ctgctcatcg ccgatcgcag cgcgaagcgc ggcgaagact
     6061 cgcgcgtcgc gatcgacgtc gaccctcctg ccccgctcgt cgtgcggctc cgctgcaagc
     6121 cgggcctcgg ctcgctgctc gtcgaggatc tcgcagcgcg cggcttcgca ccgacgccgc
     6181 acggcgacgc cgcggccgac gtcacgctgg cgcgtgcatg gtcggcgttg ttcgcatcac
     6241 ggctgtggga gcgtgccgcg attcgtgtgc cgctgcgcag cgacgaaccg cgcgcgatcg
     6301 tcgagacgct gaccgcaccg cgcatacgtg cgctgttgca ggcgtggacc gtcgggccga
     6361 tccgctggcg cctcggcttc gcgcagggcc acaagcgcgc gatcgtgtgg caggtcgcga
     6421 aggacgtcac cgcacgcgcg cccgagctag tcaacgaccc gacgcaaacg acgtgggaca
     6481 tcgccgtcac cgatgacgcg ctcgaggtct cgccgcgccg gctcgacgat ccgcggttcg
     6541 catggcgcat cgccgacatt cccgctgcct cgcatcccac ggttgccgct gcaatcgcgt
     6601 acgtcggcga ggcacgtcac ggcgaccgcg tctgggatcc gttcgtcggt tcggccgccg
     6661 agctgatcga gcgcacgcgg ctcggcacat acgcgtcgct gctcggcagc gatctcgacg
     6721 acaacgcgct cgcggccgcc cgcaacaacc tcgccgcggc taacgtcgac gcgaagctcg
     6781 tcaacgccga cgcgcgcacg tacgacccgg gcgacatcga tctcatcgtg acgaacccgc
     6841 cgctcggcag ccgggttcag gtcgacgcgg cggcgctgct cgtcgcctgc ttgccgaact
     6901 tcgcgcgtgc actcgcgccc ggcggcaggc tcgtgtggat cacccctgcg caaaagaaga
     6961 cgacgaaggt cgccgagcag ctcggcctgc gccgcacccg atcactcgcg gtcgatctgg
     7021 gcggcgtccg cggccatctc gagcgctggg agcgctagga tgtgcgcgtg cctgtagcgg
     7081 tacgaacgtg gctgcgctac gtggtgccgc tgacgttgct cgcggcgctc gcgttcttgc
     7141 cgctgctcta cgtcgcatcg cgttcgggcg cggccgcgga tctcgtgaag gcacgcgcgc
     7201 aggtgcgcct cggctggatt ctcgccggct tcgcgtggat gagcatctac ctgctggtcg
     7261 ccggcgtcgc gcctgcagtg cgtagcgtgg ccgccggtgc gccactctcg cagtggcgcg
     7321 cactcgccga tggcgcacgc aacctggtgc gcgcgttcgt gccatggctg atcgtcgtgt
     7381 tcgcggtcgc gcttggcgaa gtcgcgctcg tcatccccgg cttcttgatc ggcatcttgc
     7441 tgtcgctgac cggtgctagc gtgcggatcg gcgagccacc accggccgcg ctcgtcgata
     7501 gcgttgccgt ggtccgccgc cggttcgggc atgtcgcgtt gatcgtgatc gcgatcctgc
     7561 tcgcgaatct cgcgatctgt tacgcgctgc aggcgacgtt cgtgccgcac atcacgagga
     7621 aagtcgcggc tacgaagctg ctgccgattc gcacgttcgt ccgcttggtg cccgtcgcga
     7681 ttgccgcgct gtcgccgctt gccgcgtgcg tgctcgccgc gacgtacgga aggctcacac
     7741 gccgaacgag ctgagcgcgg cgcgcgtctc ggccgcgttc gtggcgatcg gcacgcgtgg
     7801 atccgcgttg cccttgctcg cccgcgagcg gtaccagatc gcgttccagc ccgccgcgag
     7861 ggcgccctcg acatcggcgg cccacgaatc accgacatgc acgatgacgg tgggctcgac
     7921 cgcgagcgct tcgagcgtga gcgcgaagat ccgcggatcg ggtttcgcga cgccgacgcg
     7981 acccgagtcg atgatcgtgt cgaacagtgg cgcgagctcg atctcggcga gcagctcggc
     8041 gaggtggcct tcggaattcg acagcgcggc aagcgcgacg tcgttggtcc gagcgcgcag
     8101 ctcgcgcacg atctggacca tggccgggat cggtttacgc cagagattcg ccgtggcctg
     8161 ctgttcgtag agccacgcga cgtgtggcat tgggtcggcc acgcccgcac ctgtcaagag
     8221 cgattgcatg agctcgtgcc aggggtgact ggctccggac tcggtgagcg cgtcatagcg
     8281 ctgccaggcg gttggcagtg cggcacgcag ggcatccgtg gggacggtta cgccacgttg
     8341 gcctaagcgc agggccagga agtcaaggtc gaggtcgacg agggtctggc cgatatcgaa
     8401 cgttactacc gacttcggca tcgccccctt ttacctcggg ggtggccggg gcgggtatac
     8461 tgggacggtg atgccggagg ggccttctgc ccagcaagcg ctcgtgccgg gagtcttggt
     8521 cgtgtggtcg ggggcccagc ccacggtcca ggcgtttcgc gtacccgcgt ctggcctggt
     8581 gatcggccgc gagctgctgc agaccacgac cgacgatcgg atcagccgcc agcacgcccg
     8641 catcatgtgg aaggaccggc ggttcgtcgt gaccgacctc ggcagccgca atggcaccta
     8701 tgccggcggc cacgcgctga tcgatcgcga ggtcacggtc acgccaccct cggtcgttcg
     8761 caccggccgc acggtctcgg tgctgctcga agatatccgg cgcttcgaag gcggcgcgat
     8821 ggagacgaag ttcgacgcca ttgtcggcgc gagcacgcgg catctgtgga cgcagatgga
     8881 acaggtcgcg aaggccaacc aaaatctgtt gatcatcggc gagcctggcg tcggcaaggg
     8941 ccgcatggcg cgcggctatg cgcgtgtccg taccaagccc gaagcggtgt tcaacccgac
     9001 gatccaggcg gtgccgctcg agcgcattgc tgcaccacag gtcgaaacgc tgatcctcga
     9061 gcaggtcggc aagttcggcg cgcagaacac gcagacgctc gcgaggctgc tcgagacgcg
     9121 gccgaaccta cgcatcgtga cgacagcggt gatgccgctc gagcacctcg gcctaccggc
     9181 cgaaatcgcg gtgaagctca gcagtgcgac gctgcacatg ccaccgctcc gcgatcggcc
     9241 cgacgagatg gcattcctcg ttcaggatgc ggtgcgcgcg tcggaaccag gactgcagat
     9301 tcactcgacg ctgatcgaag cgtgcttgct gaggccgtgg cccggcaact cgcgtgagct
     9361 cgtcggcgag gtcggccgca ccgcgcatac cgtcgcgtcg cagggcaaga acaacatccg
     9421 cggcgaggat ctcgacaacg acgccggtca cctgatggtc ggtgcgccga cgctcaacgc
     9481 ggcagtgacg ccgaccgccg tcggcaaaca gcgccgcaag cgggcgtcgt cgcgaccgaa
     9541 cagccgcaac gagtaaccaa gcaatcggct tcgctctggc cgaagcccga agcccgtagc
     9601 ccgaagtccg gatacacccc gcccttcccg cccgagctga ggcaggctct accactacgc
     9661 cgcctccctt ccactcacgc cacgcgaccg cagtccccat cataggtgcg gacggccgag
     9721 gttcgacgac gttcgaccgt cgacggccaa aatgactatg cggcgactac gcagcgcgac
     9781 cgagcttgcg caggagctcg cggcgattgc ggatgcgcag cttcttgagc gcgttcccga
     9841 gccgcttcgt cacggtggcg gtcgcgagcc cgagctcgta cgcgatgtac ttgcggctgt
     9901 gaccgagcgt cgcgagccac acgacatcgc gctcttgctc gtcgagcgcg agcacgtccg
     9961 ggccgtttac cggatttcgg cgcgcgagca tcaagcgctt gccgtcgcga tcgacgaaat
    10021 cgatgatcga ccaccgtccc gacaccagca ccgaccacag ctgcgcagct tcctcgggat
    10081 cggtgcggcg caagcggccg cgtgcgcgtt cgcggcgcac gacggcttcc acgagcgatt
    10141 cgcgctgtgc cttgtcgcgc gcgtcgccga tcgcgtgcag cactttgccg ttggcgtcga
    10201 ggatcgactc ggtgccatcg ccgtcgatcg cggatggtgt cgcgagctgg cgcagccggg
    10261 tcgccgcacc gagatgcgca gccgcgagtc cgagcatgcg cgcgcgggga aacggctgct
    10321 cgggatcgac gtcatcgcta gtcgccggaa acacgagcgc gaccgatttc gaccgcggat
    10381 cgccgccgat cagcgcgaac gctgacaccg ccgtgccgcg cgtcagcttg gcgaccgact
    10441 tcttgcgcat gcgcgcgatg cggcgcagcc gaaactgcga gttgccgaca aactcggtcg
    10501 gcgcgtacat gagccgcgca gtggcggcgt cgaacgagct ggtgaacgcg cgcacgcgcg
    10561 tctcgaaatc gtccgcgact ttcgtggcga cgtagctctg aatcgccggc gcgtcgccga
    10621 gctcgaccgt gtacgcggcg atgccggtgc cgatcgcgaa cgcgcgcgct gcctcggcga
    10681 cgcctgtcag ccactcgcgt tcatcttcga cccagcgata gccagcctcg acgacgcgca
    10741 gcgcgtctgg ctcagccatc gcgcaggcct ccgtagcggc gaatcagatc gaagcgcgac
    10801 gccgcaccgg tcttgcgcag aaggctcgcg acctggttcg cgaccgtgcg caccgacgtc
    10861 ttgcgcgcgc gagcgatcgc cgcgttgctc tcaccgcgaa cgacgcgggc gagcacgtcg
    10921 cgctcggcgc tcgtgagcat gggcggcggc gccgaaacat cccacgagaa gatcaccagc
    10981 tcgtcgtcga gcttctccgc cacgaggttc ggcggcgggt cgatgcgctt gccgcgcggc
    11041 atctacctcc aacgctaacc cggtcctcga cggagcagat acccgaaagg gcacgggatg
    11101 gccgaaacta tttgttgctg cggcgccggc gcaaaattcc gagcacagcg atcaacgcga
    11161 agcccatgtg gccgcctccc gacgagcaac caccgccgtc gcccggcatc atgtcggtgt
    11221 cgtcgctacc ggagcctgaa ccggtctcgg tctcgatgcg cgtcaagcgc accgcgtcga
    11281 acacgagctg cacgttgttg gcgagcggct cgcccgtgtt gtcgcccagg cgcagcgact
    11341 ggtcgccgcc ctgcgcgaaa tcgaattcgc cgagcgtctg ccagccatcg gcggcggact
    11401 ggtcgatcac cttgtcctgc atcgcaccag aggcctgcac gatgtacttc gcctgtttgc
    11461 tctgcgcgta cttcgcgtcg gtgtagacct cgagcttgta acgaccggcc tgttcgaaat
    11521 cgaagtgcca gaggccgaag ttcgcttcgg cggcgtcttc ggtcgtatgc gtccacacga
    11581 gatcgccgtc ctgccctgca tcactgaccg cgcgcatcga tgcgaccgga ccaccgggct
    11641 cgaagcacga gtcgccatcg tcgatcacgg cgcccgcgta gccgatcgac tggcacggca
    11701 gcacgggctc gggtgggccg aggaatgcct ggagcgacgc cagatcgccg ttccagcgat
    11761 cgacgtcgac atcgccgctg atgccggcga ccgttccggt ggacgtgtac tgccacagcg
    11821 cccacttcgt ccacggcggc gcgatgttcg ggcacgccgc ggtcgtgtac tgcgcgtgcc
    11881 acagcggcgt gtccgcatag tcgggcgcac cgacgctgtc gcgccagaaa tagaagccgg
    11941 tgtagatgat cggatcgcgg ccgagcgcgg ccttgacgtg atcgacccag accttgagct
    12001 tggcctggat ctgcgcggcg gtgaggccgt cgctcgcttc gacgtcgacg acgggcggca
    12061 gatcatcggc cgcgagcttg ttgccgatct tcgtgagcaa gaggtcggct tgcgcgagcg
    12121 gatcctggcc ggggcggaag aactgatacg cgccgtgctt gatccctgcc gcgcgagacg
    12181 ccgaccagta cgattcgaat tttggatcga tgaagccagt cccgtcggag acgcggatga
    12241 acgcgaactt cacgccatca cccgcgacct gggaccaatt gatcgtgccc tggtaatagg
    12301 acacgtcgat ccctttgacg gtcggcccga caccacacac ggtggcgtct tgctcgagca
    12361 ccgaaacgtc ggtggagtcc ggctgcccct cagcagcaca gcccaccatg aacgcgatcg
    12421 caattgcgcg cttcaaggta cggcaagcat cagcaagcca tacgccaa
//
LOCUS       DASUFX010000595         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1346593, whole genome shotgun sequence.
ACCESSION   DASUFX010000595 DASUFX010000000
VERSION     DASUFX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346593"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1588
                     /locus_tag="VFV99_23835"
     CDS             80..1588
                     /locus_tag="VFV99_23835"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5062432.1"
                     /translation="MSWIAGIQPAEDPATPMSLAHKAARGALWTVISSMGGRAVGVIG
                     TLVLTRFLAPSDIGEVSDAAILALTASWFTVWGFGQYTVVKGRGAAKAEVTWHATFFY
                     IVLGCFSLAICVLFGGSLTRLLGAPEAAKFVPGMALSVFIRRIGAMPERMLTQEMNFR
                     PSGLALALGEAAFTATALPLAAMGWGGWSMVVANIMKSIVAVWILVSAAGWRSWTTPT
                     KLSLARTKDMLKFGVPLGIQGVAHMASRYWDNLAISYWFGTGPLGAYNMAYNLADIPA
                     VQVGEQIALVLLPSMAELPKSRRPRALERSTALLSLVIFPLAIGLGLVAYPLIAWILP
                     ANAWQEVAPLLTVLACLSVFRPITWVLSAYMEAEQKTGRLMFLELAKVAFIVGGIGLL
                     APFGLEVAAGAVGLAFGLTALAGIAMVVRHGPSPARLAAGFLGPLAACTVMGVVVWLT
                     HHGLVSAGVHRPSILVLVQVVTGAIAYVIAALVICRETSKDLLQLVAKALKR"
     gene            1716..>2547
                     /locus_tag="VFV99_23840"
     CDS             1716..>2547
                     /locus_tag="VFV99_23840"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5062433.1"
                     /translation="MNHDLTVRFLGVRGSIATPGMAAGGNTACIEVVAGDTRIILDAG
                     TGLRTLGDERMASGIRHSTILLSHLHWDHVAGLQFFTPVYVPGHRVEIASGPNGVMSH
                     EEAIRSLFRAPFFPVDFDQLGGTVTTRELKPNDRFQIGDITITMAKLNHPDPVYGYRL
                     EHGGQSIVYATDTEHFSCVDPTLKKLAAGADILIYDSQYLPEEYPQKVGWGHSTWQAG
                     ADLARAAGVPQLVLFHHDPGRTDAQLDALEAAARAALPGTVAAREGMVLNARDAERAA
                     A"
BASE COUNT          378 a          829 c          851 g          489 t
ORIGIN      
        1 ctaccggctt cgccgctttc tctagaccgc tcagcgtagc aacggcgcac agtgcgcggt
       61 ggggtagcgt ttggcgtcgg tgagctggat tgcgggcata cagcctgcag aagatcctgc
      121 aacgccgatg tcgctcgcac acaaagctgc acgaggcgcg ctgtggacgg tgatctcgag
      181 catgggcggc cgtgcggtgg gcgtgatcgg cacgctggta ctcacgcgct tccttgcacc
      241 gtcggacatc ggcgaagttt ccgacgccgc aattctcgcg ctgacggcga gctggttcac
      301 ggtgtggggc ttcggtcagt acaccgtcgt caaaggccgc ggcgccgcga aggccgaggt
      361 gacgtggcac gcgacgttct tctacattgt tctcggttgt ttctcgctcg cgatctgcgt
      421 gctgttcggc ggctcgctca cgcgcctact cggggcgccc gaggccgcga agttcgtccc
      481 cggcatggcg ctgtcggtgt tcatccgccg catcggcgcg atgcccgagc gcatgttgac
      541 gcaggagatg aacttccgcc cgtcggggct cgcgctcgca ctcggcgagg ctgcgttcac
      601 cgcgaccgcg ttgccgctcg ccgccatggg ctggggcggt tggtcgatgg tcgtcgcgaa
      661 catcatgaag tcgatcgtcg cggtatggat cctggtcagc gccgcgggct ggcgcagctg
      721 gacgacgccg accaagctgt cgctcgcgcg cacgaaagac atgctgaagt tcggcgtgcc
      781 gctcggcatc cagggcgtcg cgcatatggc gtcgcgttac tgggacaacc tcgccatctc
      841 gtattggttc ggcaccggtc cgctcggcgc ctacaacatg gcctacaacc tcgccgacat
      901 tccggcggtg caggtcggcg aacagatcgc gctcgtgctg ttgccgtcaa tggcggagtt
      961 gccgaagagt cggcggccgc gtgcactcga acgctcgacc gcgctgctct cgctcgtcat
     1021 ctttccgctc gcgatcgggc tcgggctcgt cgcgtatccg ttgatcgcgt ggatcttgcc
     1081 ggcgaacgcg tggcaggaag tcgcgccgct gctcacggtg ctcgcgtgtc tctcggtgtt
     1141 ccggccgatc acgtgggtgc tgtccgcgta catggaagcg gagcaaaaga ccgggcgcct
     1201 catgttcctc gagctcgcga aggtcgcgtt catcgtcggc ggcatcggtc tgctcgcgcc
     1261 gttcggcttg gaggtcgccg ccggtgcggt cggtcttgcg ttcgggctca ccgcgctcgc
     1321 cggcattgcg atggtcgtgc gtcacggtcc atcaccggcg cgactcgcgg ccggctttct
     1381 cggtccactc gctgcgtgca ccgtgatggg cgtcgtcgtg tggctcacgc atcacgggct
     1441 ggtctctgct ggcgtccacc gtccatcgat cctcgtgctc gtgcaggtgg tcaccggcgc
     1501 gattgcctac gtcatcgcag cgctcgtcat ctgccgcgag acgtcgaagg acctcctaca
     1561 gctggttgca aaagcgctga agcgttgatt caaagtgatg cttcgaaacg gagcaggccg
     1621 caccaggatg gagcaatcct ccttgccgat ccaatgactt agggctggca tcgaagctgc
     1681 tgttatcccc ttcgaaagaa gaggaggtag cagctatgaa ccacgacctg actgttcgct
     1741 ttcttggtgt ccgcggctcg atcgcgacgc cgggtatggc tgcaggcggc aacaccgcct
     1801 gtatcgaggt ggtcgctggc gatacgcgca tcatcctcga tgctggcacc ggtctacgca
     1861 cgctcggcga cgagcgcatg gcgagcggca tccgccactc gacgatcctg ctctcgcacc
     1921 tgcactggga tcacgtcgcc ggtctgcagt tcttcacgcc ggtctacgtg cccggtcatc
     1981 gcgtcgagat cgcgagcggc ccgaatggcg tgatgtcgca cgaggaggcg atccgttcgc
     2041 tgttccgcgc gccgttcttc ccggtcgact tcgatcagct cggcggcacg gtgacgacgc
     2101 gcgagctcaa gccgaacgat cgattccaga tcggtgacat cacgatcacg atggcgaagc
     2161 tcaaccatcc cgatccggtc tacggctatc gcctcgagca cggcggtcag tcgatcgtgt
     2221 acgcgaccga caccgagcac ttctcgtgcg tcgacccgac gctcaagaag ctcgcggccg
     2281 gcgcggacat cctgatctac gactcgcagt atctgcccga ggagtatccg cagaaggtcg
     2341 gttgggggca ttcgacgtgg caggcgggcg ccgacctcgc acgcgccgcg ggggtgccgc
     2401 agctcgtgct gttccaccac gacccgggtc gcaccgatgc acagctcgac gcactcgagg
     2461 ccgcggcacg ggcggcgctg cccggtacgg tcgcggcacg tgaaggcatg gtgctgaacg
     2521 cgcgcgatgc ggagcgtgcc gcggcgg
//
LOCUS       DASUFX010000596        28571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1208326, whole genome shotgun sequence.
ACCESSION   DASUFX010000596 DASUFX010000000
VERSION     DASUFX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 28571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28571
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208326"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFV99_23845"
     CDS             complement(<1..1151)
                     /locus_tag="VFV99_23845"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5062434.1"
                     /translation="MAIKLDRRTVLKGIGGSILALPLLEAMTGGKRARGANPTIPKRY
                     IVCFGGQSLGADNDPIHNMYVPDTIGANYDLKVALAPLANHNNIKNEITVVSGLKIPT
                     AFDNGGAVPAGGRSDDFHINSLGPLLSGVSTNDNKVHGPTSDQLVADALGGSTTFKSL
                     VYRVQASWYLDQSAPYGRDVISYRMNGSQLVGIPSTVSPRAAFDSLFVGFTPVDPAEA
                     AKRAFLLAQRKSVLDLVSAKTEKLMPQLGTADRQRMQQHLDAVRDLERRISAIAPDGG
                     GACQLLADPGGDPAVGGNNTVNGGTGFDINAGYSDEEARATLLVDLLHMAVVCDLTRV
                     ATLQFTMAQSHMNMYPLIGIPYDLHEIGHSSYGTMGVSQGIAWHMKHFAR"
     gene            complement(1154..2731)
                     /locus_tag="VFV99_23850"
     CDS             complement(1154..2731)
                     /locus_tag="VFV99_23850"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5062435.1"
                     /translation="MRDLQWPKVCLALALVACGNDSVPRVSVGPTGARRLSRNEYDNT
                     VRDLLLDESRSGFAKLPEDVLDPFDNDFTTQQPSGILVDAAETLAFEASARLMADPAK
                     RDQVIGCVPTGADDTACLRSFVMTFGRRALRRPLTPQEVEQYVAFSSLSVEGNDFYVG
                     AGMVIAALLQDPEFLYRIERGEPVKGRTGLYRLDDYEVGTRLSYFIWGSTPDDELLDT
                     ASKGALRTPEQVRAAAERLLADPRARARIDRFHAMWLGYYKLPHSADLVASMRTETQK
                     LIERVVFEEGRPWADLFTSNETYIDANLAQLYGMPTPSGGAGWATYSGPGNRAGLLST
                     GAFLSVAGKFGDTSPTQRGRLIRERLMCQVVPPPPPNVDVDKPPTSTTMSTCKVDRYA
                     SHRSDGACYGCHNQMDPVGFGLENFDQTGAWRTTDKDDASCVISGDGQLYGLGDEPVT
                     FNGPAQLESVLIDSGVLENCLVTQLYRFAVGHREQRDDAQVIDALATEFTQTRKLDEL
                     MLDLVSADAFGYRQEEK"
     gene            complement(3131..3436)
                     /locus_tag="VFV99_23855"
     CDS             complement(3131..3436)
                     /locus_tag="VFV99_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062436.1"
                     /translation="MEHPIDLAECVSTGDRRVEASPARVDDDAAVFDDHLARITDALG
                     RERYLPPRGGAGVALDLIDAEANEATELTALVTSGIFVELQFHTRTHSEVCERQHCG"
     gene            3591..3962
                     /locus_tag="VFV99_23860"
     CDS             3591..3962
                     /locus_tag="VFV99_23860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062437.1"
                     /translation="MRDSEVRRWIEHEMFGERVSVDFVDAVPDILTSLTLVPPPWPQL
                     LIIDLDAIARAEVDQLATIREAGWPGMVIALGDPGVTVQRSLDIDIVVPRSLKCEQLR
                     NALKELRSNRAALSAHHRRAG"
     gene            complement(3967..4773)
                     /locus_tag="VFV99_23865"
     CDS             complement(3967..4773)
                     /locus_tag="VFV99_23865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5062438.1"
                     /translation="MCKPTPGQIHIEQTAAFAADSLRGRPRVLELGSRGELATRLASL
                     GFSVTTFRDGEPSAGVTRLDTDLSTYEGDPFDAIAMTNVLQREQDLDAFLDDVYAHLK
                     PGGRLIIEELDVTAPDVRTLRWYYDAIARITLAMPTADADVEVCWGAEWSGCHLHGAS
                     RIEQAVTRRFSIRSLHRVEYLYREVARRLLDTDEGRRIALALRQIERQKVADGSIVAT
                     GIQLVADRSPETRAAVCGARLTAEVSCGLAPGHDGLHQWRGPNGQTFDWG"
     gene            5021..5794
                     /locus_tag="VFV99_23870"
     CDS             5021..5794
                     /locus_tag="VFV99_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062439.1"
                     /translation="MRLPHLALAIVAAAGCAKGGDGGEDEMVDGSCGDRCDADSDGVF
                     DPMDMCANTPSGEMVNTKGCSDSQLTPTLEPTFPPYNLMWTMGGDIGRPGGLTWTYDG
                     IQRGDLFHIYWIICDDPLQPCGISLDGPIDKPSESWKFSATDSDLPAGKVAFTNITAI
                     ALDGGTNQALDGRLTVTITSATDVALPFATVNALGVPARAGTYGAEIPGTGYKVAALI
                     EVRDTSGTWTPYLDYYDAAPTPMTGGSTSVSFGASFYSK"
     gene            complement(5804..6148)
                     /locus_tag="VFV99_23875"
     CDS             complement(5804..6148)
                     /locus_tag="VFV99_23875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062440.1"
                     /translation="MTRRLAFIVLLAACSDSGAPADATTARPDVDVAACGSIGMACTT
                     TCPAGLECISTNVCASVRGDCGGFSGAMCQDTSLTCTYPTGSSAGHCMRDDEKACLCA
                     IAPNALGDCMQP"
     gene            6314..9568
                     /locus_tag="VFV99_23880"
     CDS             6314..9568
                     /locus_tag="VFV99_23880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5062441.1"
                     /translation="MPRCCLLFVLSACAITHYPPPAPPRTTHVGLAISTTTLANGQRV
                     VLVNDPHAIDVQVTMRYQVGSINDGAQPGIAHFVEHLMFEQILDGQPLFTHYEDTASF
                     FNAYTNFEATTYVARGPAEQLDKLLALEAARLSLPCNTITDAAFAREREVVINELAER
                     DQTTEVYGAIHGVLFPKDHPYRQAIGGSETSIRAITREQACAFASMYYAPSNAVLVVS
                     GNIGDRARAAVAPLGRVAPGRRTPAPRSVPRVALHPIHVDVPAPVDEDILVLAWPLPT
                     DPELQVKVRAVAAALPRLVDNEIKGTVASVEFGDRGAPMFGLAVLPAEGETFQDATSA
                     TRKGIEQLPALYVEEDEKSDVLFDRMREGAVYALYSGLEDGSDRDSRLAAYVLAGRDP
                     NRAFAAELDALRGMTRDEAAAIATQYLAANAPTVLTLKAATGKKSGQTLALHKPIHDI
                     GPRRKPPDPALARKPADITAPNQIIDAKTRVLPNGLNVVLLPMSSVPTVDIRLIFPTG
                     MADEAPNQRGLANLTAHALTWDLHHFNDVLLFAAAGGLKDVDVGVDRTTFSVQGMDTK
                     LDVLLAGLRRLVREGTFDDDSAELVSTMRGAAKRADDEGELTDTWRAAVFGADHPYVR
                     AGIARYSNSALTLDDAAQFRAIHYTPTNATLVIAGSFDPALADRWIDFLFADWRGPQV
                     YRQTPRSKPAPASIGKADDLTMLQLRIAIPAIAGGRPEQLVAAEMLGEIARDIRFELA
                     AGYSVNAQLSERRLARNYLLGGYVDIARSSAAIELIRDRVTQLRNDNDAAARAFVTAR
                     KHVIAQLLSRVGSAGSLAQRVEHDVEMERDPMSDLKTAATVRALTLDTMAATLADIDL
                     SRATVMMRGPSAELKQAFDILGRKASYVLEDPFKAQAADDSAPGATGVLGTIAMPVRK
                     SDVEPALTLQPPPRLALTIAVNALAGLLFEGSEFYPALGESVQVHAGYRYMPDRSIGA
                     RLAVGKFAGSYGAGEPGLDFTAVPIDIGAVIGFDFSKKRSYLEANVGLRLDRVSDSMP
                     TRTYKGLGYGAILGRNLARIGGSWLALAFGFNAMSYRTSDSGATLLTVGLVLRR"
     gene            9741..10571
                     /locus_tag="VFV99_23885"
     CDS             9741..10571
                     /locus_tag="VFV99_23885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062442.1"
                     /translation="MTCVPKTCAQRGAECGTTDDGCGHSLDCGYCLFQSDACESNQCV
                     CQPSCYMRQCGDDGCGGTCGSCGANESCNQYGKCIYEGAGTTPPATWHCNAAYWDAGD
                     GCDCNCGALDPDCGQSGQSLHGCTGLNSPTCDAQGLCTGGGACHSVPGSAYLTHYMVI
                     QSAPTMYGGTVASGRYEGTDMHSYNPPGGISGYAGGDGVAIEINGTTWKRMRRPVGGY
                     PPVDETFTASFSGTTVTLTRTCPSALTETFTYTASSTQLVLSQPSGSGTKVITFTKRW
                     "
     gene            complement(10604..11278)
                     /locus_tag="VFV99_23890"
     CDS             complement(10604..11278)
                     /locus_tag="VFV99_23890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062443.1"
                     /translation="MRKYQRLTAFSRVIAAMVCGCSSTPDVDSTPCVQLESAPHDDFE
                     QPTASPCSWGQLSQLDCTATVENGDLVMAPKPGAGDVFCVCGATQLIPFTSTFGTIAE
                     VHDVATEVGEDSVLVALASVEAWRVGYDAAMDMLFFEHIMGDERTRFGYAPWDPAAVW
                     WRVRLDEDAVIGEVSADGERWVRIGADHTRPPPEMILPSFMTLTSQPVAAPSTVRIGS
                     FNVCPP"
     gene            complement(11301..11741)
                     /locus_tag="VFV99_23895"
     CDS             complement(11301..11741)
                     /locus_tag="VFV99_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062444.1"
                     /translation="MSDAADGTDNGDARRDAGVTETRVTGVMYGMPFTLQYASIKRAS
                     STDPLNWVCVADVPSTYDQCSMTGGPERFVFLGPFIYENGVPKWAIVQVWLYHVGTSY
                     SKSADSGMFQILVDNPTTGEFQLTLDVDFAETPHTVGSVLIPGA"
     gene            complement(11874..12521)
                     /locus_tag="VFV99_23900"
     CDS             complement(11874..12521)
                     /locus_tag="VFV99_23900"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5062445.1"
                     /translation="MKLANLFTAACLVAGLAAPACKKEEDKAPTPATGSAGSSASVTE
                     PASEPDARADHITVLAHHKNPKPSDPVRINFEKFRVVKADFDPQKIEGGTATIEIALS
                     SFRTDSDERDEHLKSPAYLDVAKLATATITIANVKQKAGATYAADATVVAHGITKTYP
                     ITFDVLDRKADRITIKGTHTFGRLDFGIGSDPAQNPEEQVGTDVTIEMVLTITKS"
     gene            complement(12542..13459)
                     /locus_tag="VFV99_23905"
     CDS             complement(12542..13459)
                     /locus_tag="VFV99_23905"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5062446.1"
                     /translation="MRWYVQGSGNVYTALAKRFYVPDPDLGWRISTQHPIWLGLDVCA
                     AIAGLMLVLAVVGWWIRRRTSQPARGRLLRVGSWCLAAISLCVPAAAFVSGAGPLHAR
                     DTLPATAAVLIEDGIEGSLDAPEGSYVVVTHAGTAVTAHLSAGGEAFDARFGDVTGTW
                     RATPRDLRRPLHAEISVAAASVDTGVGERSKHAREGYLHADEFPRIGVTLDHAAAVRA
                     NGPRELAFRAPATVSLMGRTHAIDVTGTLTKLDAAPLARLGLAGDVLLVQADFALVTR
                     DTALAPKAHDLDGERIPIHVSLVLRHTTD"
     gene            13679..14422
                     /locus_tag="VFV99_23910"
     CDS             13679..14422
                     /locus_tag="VFV99_23910"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX5062447.1"
                     /translation="MKPVRPIAKPTAPPSDDWHPRTIIIVAAVTVFVISAWVPFGSTA
                     LYPLTLFTTWVHEMGHGLTALAFGGEFRSLEINANAGGTAYAFAGHGWPDAMVAAGGL
                     LAPPFLGALLLAFVHRPRAARIVLGVLAAALVLSLAIWVRSAAGIIAMPLVAALLGWA
                     AWRGFAENPGRRVVLVQVLSVILGLDTLTRMVGYAFMDKTSLGQRSDVSAIADNVGGH
                     YLLWGLVITTLALGMLALGLWRAWRRPAP"
     gene            14463..15251
                     /locus_tag="VFV99_23915"
     CDS             14463..15251
                     /locus_tag="VFV99_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062448.1"
                     /translation="MVQRVLAIVCALSAVALADSYPMRSTRKLEVGIHEGVPWPLANA
                     PIDGRLPDAPVSRDAQPDAPTKAGKRVLEVIDGIEESLRDTSYQARTSVRADKGEYHW
                     DCSGMAAWILRRAAPAALRKLSSERPVARDFADAIEHAPAKRAKDGWQRIANIADVMP
                     GDVFAWRRPRGMPSKNTGHVGFVVERPLRVPEIPGAWAVRIADSTRGWHQNDTRTDHD
                     GGGFGIGTLLFLADADGNVTQYGWSGTWSEWYVVTHVVFGRLHR"
     gene            15330..16295
                     /locus_tag="VFV99_23920"
     CDS             15330..16295
                     /locus_tag="VFV99_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062449.1"
                     /translation="MMGLERLSLACIVLVGCYSPELRPGSPCSTDSQCPSSLRCVAGV
                     CGGSAVDFDAPSGGGSDVGIDIDAPLGDPDPDGDGIVGAADNCPNVANPDQGNEDGDK
                     FGDACDPCPIEANDSPTDPDGDGVADSCDPMPNMAGNKIVLFEGFHNALPAGWMLASA
                     TKVGDDVRIVASGTNHSFISPPLDATTKVTIMTRVVVEQTTGNNDADFGVSVPYNPAN
                     DDSIRCELYSPTAQQTANRDLSIWDEIRSTEIGNQSFAWVTGTPYLLSLQRADTTYTC
                     RASAVNGTPVMASGTSSAVPSQQKFALRTYATSSLISYVLIVANP"
     gene            16508..18268
                     /locus_tag="VFV99_23925"
     CDS             16508..18268
                     /locus_tag="VFV99_23925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062450.1"
                     /translation="MAKPTAQVETKPSIDADAVLSIEDLRSPIRTEQEQILVDLIANT
                     ADSEVDEKTDYYFRLGELYAKQHRFWRMKSVELATATDATRKKQSAQAADQAKTYLLK
                     AVKTFKGLTDNDAFRNYPKLDTALFYYAYTLQGGKYMKEARQVYDKLLKNYPSSKFVP
                     EAHLAFGDYYYEAGQLADAEARYKQVLKFPKSTVYAYALYKLGWIHLQLSRPQEATEA
                     FYQVVAATRGDARQASLNHAAKTDFARAYAGIGKAEHAFDAFQKLDKSAAVELAELLA
                     DISRDKGDSNRAVTAYRDLLLRAPGDARACRWQYRIAHATLSIPGATNAVKVKEVEAL
                     VHSYTSQKQPDAECRANAAAMSAELAAAYHAEWSKTQNVETLGYAEQLYAMHVAAFPD
                     AAAQRETYAEVLWSRADHESTAKVRAERWQRTADVFATIHTQEAARATALAWMNALDI
                     TTPADTKVVIGKPPRSRPRALPLKGLEAKLVAAVTAYTQQSPDADEDLAQMRLATATI
                     LRRNRHFDEAAAMLDEFLEHHPDHALAELAANLMLDSLVKAARLDDALQVADAIATDR
                     AFVEGKQELQRNIQLLRLLH"
     gene            18337..21987
                     /locus_tag="VFV99_23930"
     CDS             18337..21987
                     /locus_tag="VFV99_23930"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5062451.1"
                     /translation="MEKEADTRPAGSSSGGGKSNREFSGTDRFQLIRQLGMGGMGVVY
                     EALDRESGEKVALKTLRNVDGNAILLLKQEFRALAGVHHPNLIRLGELFESRGDWFFT
                     MELIRGVDLLSWVRDGDSQRLRDTLAQLVTGLGALHDAGHIHRDIKPSNVLVSDGRVI
                     LLDFGLATRHDHDQLSWSDGAMVGTPDYMAPEQASARPAGPEADFYAVGVILYEALTG
                     RLPIQGHALEVLMEKQRIVPAAPSTLTSVPADLDALCCELLQIDPATRPSRNEIMRRL
                     AATPRADSPTALTRDAATMTPMPRQTFVGRETELGVLRDALAECDTGVPATLLIEAES
                     GMGKSALVEHFVAGLDRRDVVVLRGRCYENESVPYKGVDGVIDSISRFMARLPDKEAA
                     ALLPFHIGLLAEVFPVLRGVSVIVEQPPVQHVPDPQEQRRRVFAAVRELLARIGARHP
                     LVIMIDDLHWADEDSLALLDAVLRPPDAPCLLLVSTAWPRGDEPPIQFGSNLRRIVLE
                     PLDEQETTRLAHALFTAVRAPIAEATTATIVRESAGHPMFVAELVRHTVLRGDTGTTA
                     SEIRLDDVLAERIDALETGERQLLELIAVAGQPLPLEVAGRALGLTPSKLLPLIGYLQ
                     SANLLRATGRTRRDSVEPFHDRTRRAVTARSAERKPDLHRRLAVALEATDAETQPMLV
                     GVHWEQAGEPARAAALYARAGDRATEALAFERAVKLYERCLALAPELSPVLRAKLGDA
                     YANAGRGREAAEVYLALARESKIAADALELEQKAAHQLLRGGHIDDGMVVLERVLASV
                     DIDLPKTPRRALASLLWRRARMRLRGTSFKPRDSSQVTREELARIDIVWSAACGLSMT
                     DWIRGASFQALNLLLSLNAGETERARRALFLEACHVAAGGAPDRAETLVKAALAGGDP
                     DDPYLRGWLELARAYIAYFRGSWRKAFEGAERADALFSANCANVVWERDTVHALSHWS
                     QVYLGSLKTLGQMLPARLHEAQLQGNLYAVWALASTVSVLHWIARDDVAGGRAAVDRI
                     SERWSLRGYQIQHWNEMQSNALLDIYAGDVREARRRLEDGWVPMSRSLLTKIQVIRFE
                     VNELHVRTALAAARVSSGSEREQLLKIAEHHTDKLAREGLAWTDAITTLRRAGIRNAR
                     EDADGAVREYRAAVTACDAAELGMHAAAARVRLGALLGGDEGGALREAGLANLRAEEV
                     KQPDAFVALFAP"
     gene            complement(21991..22719)
                     /locus_tag="VFV99_23935"
     CDS             complement(21991..22719)
                     /locus_tag="VFV99_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062452.1"
                     /translation="MNILRSIGLALLFGLGVLVALIASIWIFFRSLRRGGRAFHPHGS
                     VCRVEVTALDDVVGPRLAGPARIRMASSTAEENSPSPSIIGMGIKLGSDQDLAAGTFE
                     SFLHLEDATKHTNVADYLANQYGSVAPWRARGLGVIWFRLIPDPAASAPKTGTRTERL
                     DADIAAGRAKFILEAREAPGPDGPVRSRLAELRLIERLPDDDPTFQISMFRTGRGVVP
                     TGFRNGVRAILYPVSQFARGLRGG"
     gene            complement(22716..24233)
                     /locus_tag="VFV99_23940"
     CDS             complement(22716..24233)
                     /locus_tag="VFV99_23940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062453.1"
                     /translation="MAAATVTASEQPQETLEAEDIQGLVLRGYGPLAAARFFLLEVVN
                     DDRARDYLQKLCGRLNLASISPETTALQVAFTAQGLARLGVPKSALDTFSREFREGMD
                     DNVRPDILGDRGANDPSTWEWGRQPEPIHVLLMVYALDDTTLTQVLETERPSIEAGFK
                     IRHEKETITLTGQKEHFGWRDGISMPKIAGVPPAVPAVAPAERPQKKRKESWTDPLPP
                     GEFVLGYRNDYQAFTECPTAELADDPANHLPVIVVGNETKKSLGRNGTYLVYREMTQH
                     VIELWDYLEKSSREPGKDPAARAIALGAKIVGRWPGGAPLITSPERDDDKRADENDFV
                     YEGDESGLRCPHGAHIRRSNPRDVLAVEDRSHDASKQMVRKHQMIRRGRAFGRPVAET
                     MNPRDILAARGKPDHERRGLHFICLVGSIERQFEFVQRNWVHSANFDSLFKDADPITA
                     ARRSSADENPNDEFTCPADPVRRKYKAMPPFTRLVGGGYFFLPGIAALRFISRHP"
     gene            complement(24215..25567)
                     /locus_tag="VFV99_23945"
     CDS             complement(24215..25567)
                     /locus_tag="VFV99_23945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062454.1"
                     /translation="MHKVNAGLTVLVRLKTDCVDAASALLVELDAHQERLPFAKSATT
                     HFATITVIPAQKYGDKPLPAMLLFATSFCGPTRVHVSELVRIMGDGLREVLQHCERFE
                     PSCSDDELEDFLIYHRHGDTFYSGMQGLSPADVQQHRQLREAIEEFIDDREAHGGFNG
                     SPLVVRNEIQKFVLSRPDLAWAEQPVEESTRAWLVFHWRSLIIEAFLAALIFCTGAWF
                     GGSSLFGVIAACGWIAVLAFVVFVAVMWLSIFEAEHDQTYVAARTPDDRARFLAATQT
                     RPVINEFTLAGPIKEEGMLRPAFVKVSLWVIARVVEGVPWMPYVGSGINIPTVATARW
                     IAADGGRRLMFISNYTNEGIAYVRDFIETRGGAMRINLSFGFGRGFPKTRWLLWDGAL
                     TDPNAYLYALSEHQRRTLFWYGPYRDISIDNIKRNRQIREGLFADYNEDQAKKWLQLL
                     "
     gene            25685..26509
                     /locus_tag="VFV99_23950"
     CDS             25685..26509
                     /locus_tag="VFV99_23950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5062455.1"
                     /translation="MDFVDANGIRFAYLSEGSGPLVLLLHGFPDTPYTWERVMPEVAR
                     AGYRAVAPFMRGYHPTSIPKDGKYDGDTLGRDVLALIEALGEQQAIVVGHDWGALAAY
                     TAAAVGPERVRALITVGVPHPRSVLPTPRVLWTVRHFFVLRNKGAAHKIRAKNHAYID
                     QLVRRWSPAWKDMPADETARVKQAFAEPGCLEAACSYYAALTPFMPASLRASIKVPSV
                     AFAGIHDNMSPRVYEKARRCFVGSYEVVQVPGGHFMHREHPNEFTAELTRVLRAAG"
     gene            26601..26786
                     /locus_tag="VFV99_23955"
     CDS             26601..26786
                     /locus_tag="VFV99_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062456.1"
                     /translation="MGKPPSQILELGPSPNIDHEKDMAENGAPPKATRAIYWGIIIAL
                     VVLFALVGIGLHIPTGG"
     gene            26852..28375
                     /locus_tag="VFV99_23960"
     CDS             26852..28375
                     /locus_tag="VFV99_23960"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX5062457.1"
                     /translation="MLGAIACGGSSKRHEPTAPELVAFVDGKSVFGPATEWMKSDLPD
                     VGETGLFPARTPEQCEVAVLHSGEDSFAARMALLSRAKHSIRIEALIFTGDEAGLRVA
                     EVLKQKKAAGVDVKVIVDGLSNSSLQTQWMYFDLKQHGIEVEGYEAIGLQLINEIPNP
                     LVTPWHRDPARPNSRYHEKLWIVDPDEPDAEAVMGGLNIANEYFRVDPTNVPRYWRDQ
                     DVIVRGVVLADLTATFDRNFAHFKDLKRDRGGLTDAAWAATRKIMDKTGAPTIAFERR
                     DELVRTVAAFEARTPDLDYQPAQCRFLWSRPRLHESYIQQAYLKLLATARKEVLIGNA
                     YFVPTPSVRLAIEKAARRCVRVSLLSNGPETNDEPGMNLLSRAYYAELLAVNASPEVT
                     ACKQPAGVEIWEWQGKAASDATQTQGLYHAKFAVVDRTLSLVGSYNLDPRSEIHNSET
                     AIVFEQPALAAQLISLYEHDLTTSRRITDAEAAGFEKPDSVQRRMKKDFAALFESQL"
     gene            complement(28378..>28571)
                     /locus_tag="VFV99_23965"
     CDS             complement(28378..>28571)
                     /locus_tag="VFV99_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062458.1"
                     /translation="KLSLAGGALVYSAFLFVIGMSAWLIFGLGAWLALGASSVALVIG
                     VLAKTPAPAVAPATAPAKP"
BASE COUNT         5036 a         9450 c         9208 g         4877 t
ORIGIN      
        1 cgcgcgaagt gcttcatgtg ccacgcgatg ccctgcgata cgcccatcgt gccgtacgag
       61 ctgtggccga tctcgtgcag gtcgtacggg atgccgatca gcgggtacat gttcatgtgc
      121 gactgcgcca tcgtgaactg cagcgtcgca acccgcgtca ggtcgcagac gaccgccatg
      181 tgcaacagat cgacgagcag cgtcgcacgc gcttcctcgt ccgagtagcc cgcgttgatg
      241 tcgaagccgg tgccgccgtt gacggtgttg ttgccaccga ccgccggatc gccgccggga
      301 tccgcaagca gctggcatgc gccgccaccg tcgggcgcga tcgccgagat gcggcgctcg
      361 aggtcgcgca ctgcgtcgag gtgctgctgc atgcgctggc gatccgcggt gccgagctgg
      421 ggcatcagct tctcggtctt cgcgctgacg agatcgagca cgctcttgcg ctgtgccagc
      481 aggaacgcgc gcttcgcagc ttcggccgga tcgactggcg tgaacccgac gaacagcgag
      541 tcgaacgcgg cgcgcggtga cacggtcgac ggaatgccaa cgagttgcga accgttcatg
      601 cgatacgaga tcacgtcgcg gccatacggc gccgactgat cgaggtacca cgacgcctgc
      661 actcgataga cgagcgactt gaacgtcgtc gaaccaccga gcgcatctgc gacgagctga
      721 tccgacgtcg gcccgtgcac cttgttgtcg ttcgtggata cgcccgagag cagcggtccg
      781 agcgagttga tgtggaagtc gtccgagcgg ccgcctgccg gcaccgcgcc gccgttgtcg
      841 aacgcggtcg ggatcttgag gcccgacacg accgtgatct cgttcttgat gttgttgtgg
      901 ttcgcgagcg gtgcgagcgc aaccttgaga tcgtagttcg cgccgatcgt gtccggcacg
      961 tacatgttgt gaatcggatc gttatcggcg ccgagcgact gaccgccgaa gcacacgatg
     1021 tagcgtttcg ggatcgtcgg gttcgcaccg cgggcgcgct taccacctgt catcgcctcg
     1081 agtagtggga gcgcgaggat cgagccaccg attcccttga ggaccgtgcg tcggtcgagc
     1141 ttgatcgcca tggctacttc tcctcctggc ggtagccgaa cgcgtcggcc gagacgaggt
     1201 cgagcatcag ctcgtccaac ttgcgggtct gcgtgaactc ggtcgcgagc gcgtcgatca
     1261 cttgcgcgtc gtcgcgctgc tcgcggtggc ccaccgcgaa gcggtagagc tgcgtgacga
     1321 ggcagttctc gagaacgccg ctatcgatga ggacggactc gagctgagcg ggtccgttga
     1381 acgtgactgg ctcgtcgccg agaccgtaca gctggccatc gccgctgatc acgcagctcg
     1441 cgtcgtcctt gtcggtcgtg cgccaggcgc cggtctgatc gaagttctcg agcccgaagc
     1501 cgaccggatc catctggttg tggcagccat agcaagcgcc gtcgctgcgg tgcgatgcat
     1561 accgatcgac cttgcacgtc gacatcgtcg tcgaggtcgg cggtttgtcg acgtcgacgt
     1621 tcggcggtgg cggcggcacg acctgacaca tcaaccgctc gcggatcagc ctgccgcgtt
     1681 gggtcgggct ggtgtcgccg aacttgccgg cgacggagag aaacgcgccg gtcgacagca
     1741 agccggctcg attacccggc ccgctgtacg ttgcccagcc ggcaccgccg cttggtgtcg
     1801 gcatgccata gagctgcgcg aggttggcgt cgatatatgt ctcgttggac gtgaacagat
     1861 ccgcccacgg cctcccctct tcgaacacga cacgctcgat cagcttctgc gtctcggtac
     1921 gcatcgacgc gacgaggtcc gccgagtgcg gcaacttgta gtagcccagc cacatcgcgt
     1981 ggaaccgatc gatgcgagca cgcgcgcgcg gatcggcaag caaacgctcg gccgcagcac
     2041 gcacctgttc cggcgtgcgc agcgctccct tcgatgcagt gtcgagcagc tcgtcgtcgg
     2101 gcgtcgaacc ccagatgaag tacgacaggc gggtgcccac ctcgtagtcg tcgagccggt
     2161 agagccccgt gcggcccttc acgggctcgc cccgctcgat gcgatagaga aactcgggat
     2221 cctgcagaag cgccgcgatc accatgccag cgccgacata gaagtcgttg ccttcgaccg
     2281 ataacgacga gaacgcgacg tattgctcga cctcttgggg cgtgagcggc ctacgcagtg
     2341 cgcgtcgccc gaacgtcatc acgaacgaac gcaagcacgc ggtgtcatcg gcgccagtcg
     2401 gaacgcatcc gatcacctga tcgcgcttcg ccggatcggc catcaaacgg gccgacgcct
     2461 cgaacgcgag tgtctcagcc gcgtcgacga ggattcccga cggctgctgg gtcgtgaagt
     2521 cgttgtcgaa tggatcgagt acgtcttcgg gcagcttcgc aaagcccgag cgcgactcgt
     2581 cgagcaacag atcgcgcacc gtgttgtcgt attcgttgcg cgacaaccgg cgcgcgccgg
     2641 tcggcccaac ggagacgcgg gggacactgt cattgccgca cgcaacaagt gcgagcgcga
     2701 gacagacctt cggccattgg aggtccctca tcacctccga tagtcgcact acgtgcgatg
     2761 ctttgagctc aaagattatc cgctgtcagc caatgccaga acgcggcctc gagcttgtaa
     2821 caacagtgga accacctgtg cgtcgactct gattgtgagc cgcaatgcta tgccgggagc
     2881 tcgtaaccaa tctgcgattc gatctcgagc gtcttcttga ccgcaggccg cggaaactcg
     2941 tagcgggcga tttcgcggcg cgaccgcact ccgagcgcag gcgcccatgg cccttagtgc
     3001 accgcagacc acaacaatcg acggttgcgc agcgtaagtc gtgcaagacg gtgtgcgctg
     3061 tcgacacgcg gccgacatcg agcaagtgga cgattcgacc aggctcggtt cggcatcggg
     3121 gttgctctga tcacccgcaa tgctgtcgct cacaaacctc gctgtgggtc cgcgtgtgga
     3181 attgcaactc gacgaatatg ccgctcgtca ccaacgccgt gagctcggtg gcttcgttcg
     3241 cttctgcatc gatcagatcg agcgcgacgc cggccccgcc acgtggtgga aggtaacgct
     3301 cgcgcccgag agcgtctgtt attcgtgcga ggtgatcgtc gaacaccgcg gcgtcgtcgt
     3361 ccacgcgagc gggactggct tcgacgcggc ggtcgccggt cgagacgcat tctgcaaggt
     3421 cgatcgggtg ctccacgaga cgcgtgtaga ccttgctcgt ctgggatcgg aggtcgcctg
     3481 tgtcactcgc tagagcatca gatcgggttt cgtggtcgat gttcgttgca gcgcaccgcg
     3541 cccagccgcg gcgccgtctc ggcgctacca ccgaccgggt tctcgtgttg gtgcgtgact
     3601 ctgaggttcg gcgctggatc gagcacgaga tgttcggcga gcgcgtttcc gtcgacttcg
     3661 tcgatgcggt ccccgacatc ctgacgagct tgacgctcgt cccaccgccg tggccgcagt
     3721 tgttgatcat cgatctcgac gcgattgcac gggctgaggt cgatcagctc gccacgatcc
     3781 gcgaggctgg ctggccggga atggtcattg cgctcggcga ccccggtgtg accgtgcaac
     3841 gatcactgga catcgacatc gtcgtgccgc gctcgctcaa atgcgagcag ctgcgcaatg
     3901 ccctcaagga gctccgatcc aatcgtgccg cgctgtcggc gcaccatcgc cgcgcgggct
     3961 agcgagctag ccccagtcga acgtctggcc attcggaccg cgccactgat gcagtccatc
     4021 gtgccccggc gctagcccgc acgaaacttc ggcagtgagt cgtgcgccgc agacagccgc
     4081 acgcgtttcc ggggaccgat cagcgaccag ctggatcccg gtagcaacga tgctgccatc
     4141 ggcaaccttc tgccgttcga tctggcgcag tgccaacgcg atccggcgcc cctcgtcggt
     4201 gtccagtaga cggcgcgcga cctcgcgata gaggtactcc acccggtgta gcgatcggat
     4261 cgagaaccgg cgagtgaccg cttgctcgat tcgagacgca ccgtgcaagt ggcagccact
     4321 ccactcggcg ccccagcaca cctcgacgtc ggcgtccgca gtcggcatcg caagcgtgat
     4381 ccgcgcgatc gcgtcgtagt accaacggag tgttctgacg tcaggcgcgg tgacgtcgag
     4441 ctcttcgatg atgagacgtc cgcccggctt caagtgggca tagacgtcat cgagaaacgc
     4501 gtccagatcc tgttcgcgct gcaggacatt ggtcatcgcg atcgcatcga atggatcgcc
     4561 ctcgtacgtc gagagatcgg tgtcgaggcg cgtgacgcca gccgaaggct ctccatcacg
     4621 aaaggtggtc acgctgaagc caagcgaggc gagacgggtg gcgagctcgc cacgcgatcc
     4681 gagctcgagc acgcgtggcc gaccgcgcag ggagtccgcc gcgaaggcgg ccgtttgttc
     4741 gatgtgaatc tgaccgggcg tgggcttgca catggtagtc ggcctgcgtt gcgaacatcc
     4801 ccgagggcgg cggcatcgta acctgatcga agggccatgc gttcgcggca gctcacaatc
     4861 cacgcggcta atggccgacg cgtgacgact gcgcggtgat gatttccgtg ggcacgggga
     4921 tatcggcgag agtcggcgtg actcggtcgt gaccgatctc ccggcccaaa cgacgtcttg
     4981 ttgatcccga tcggacacta tgcaacggtc gaaagcgaac atgcgcctcc cccacctcgc
     5041 cctcgcgatt gtcgccgccg ctggctgcgc caaaggtggc gacggcggcg aagacgaaat
     5101 ggtcgacggt tcgtgtggcg atcgctgcga cgccgatagc gacggcgtgt tcgatcccat
     5161 ggacatgtgc gcgaatacgc cgtcgggcga gatggtcaac accaaggggt gctcggactc
     5221 gcagctcacg ccaacgctcg agccgacgtt cccgccctac aacctcatgt ggacgatggg
     5281 cggcgacatc ggtcgacccg gcgggctgac gtggacctac gatggcatcc agcgcggcga
     5341 cctcttccac atctactgga tcatctgtga cgacccgctg cagccgtgcg gcatctcgct
     5401 cgacggcccg atcgacaagc cctccgagag ctggaagttc agcgcgacgg attccgatct
     5461 gccggccggc aaggtcgcct tcaccaacat caccgcgatc gcgctcgacg gtggaaccaa
     5521 ccaggcactc gatggtcgcc tgaccgttac gatcacatcg gcgacggatg tcgcgcttcc
     5581 gttcgccacc gtgaacgcgc tcggtgtgcc cgcgcgtgca ggtacctacg gcgccgagat
     5641 tccaggaacc ggttacaagg tcgccgcgct gatcgaagtg agggacacgt cggggacgtg
     5701 gacgccatac ctcgactact acgacgctgc ccccacgccg atgacgggtg gctcgacatc
     5761 ggtttcgttc ggcgcgtcgt tctattcgaa gtagtcgccg cgactacggc tgcatgcaat
     5821 cgccaagtgc attcggcgcg atcgcgcaca ggcacgcctt ttcgtcgtca cgcatgcaat
     5881 gaccggcgct actgcccgtc ggatacgtgc acgtgaggga ggtgtcctga cacatggcgc
     5941 cggagaagcc gccgcaatcg ccccgcaccg acgcgcagac attcgtcgag atgcactcga
     6001 gacccgcagg gcacgtcgtc gtgcaggcca tgccgatcga tccgcacgct gcgacgtcaa
     6061 catcgggccg cgcggttgtc gcgtcagcgg gcgctcccga atcgctacat gccgcaagca
     6121 gcacgatgaa ggcgagccgc cgagtcatgc gggcatcgta actcggttcg gccaggctcg
     6181 cgtgcagcgc gtagcgtggt caaacgccgg gactgtgacc aggcgcaaca gttcgcttca
     6241 cctacatcgc gccgacgatt gaacgtgtgc ggctgcgatt caatccgcgc ggcgtagcca
     6301 cgatactagg cacgtgcctc gttgttgcct gttgtttgtg ctcagcgcct gcgcgatcac
     6361 acactatccg ccgcctgccc caccgcgcac gacgcacgtc ggtctagcga tctccacgac
     6421 gacgctcgcg aacggccaac gcgtcgtgct ggtcaacgac ccacatgcga tcgacgtgca
     6481 agtcacgatg cgataccaag tgggatcgat caacgacggt gcccaaccgg gcatcgcgca
     6541 cttcgtcgag cacttgatgt tcgaacagat cctcgacggg cagccgctgt tcacccacta
     6601 cgaggacacg gcatcattct tcaatgcgta caccaacttc gaagcgacca cgtatgtagc
     6661 gcgtgggccc gccgagcagc tcgacaagct gctcgcactc gaagcagcgc ggctgtcgtt
     6721 gccctgcaac acgatcaccg atgcggcgtt cgcgcgcgaa cgcgaagtcg tgatcaacga
     6781 gctggccgag cgtgatcaaa cgaccgaagt atacggcgcg atccacggcg tgctctttcc
     6841 caaagaccat ccctatcgcc aagcgattgg tggtagcgaa acgagtatcc gcgcgatcac
     6901 gcgtgagcag gcgtgcgcgt tcgcgtcgat gtactacgca ccaagcaacg cggtgctcgt
     6961 cgtctcgggc aacatcggcg accgcgcacg ggctgctgtc gccccgctcg gcagagttgc
     7021 tccgggccgt cgcactccgg cgccgcgtag cgtgcccagg gtcgcgcttc atcctatcca
     7081 cgtcgatgtg cccgccccgg tcgacgaaga catcctcgta ctcgcctggc cactgcctac
     7141 cgatcccgag cttcaggtga aagtgcgtgc agtcgcggcg gctctgccgc ggctcgtcga
     7201 caacgagatc aaaggcacgg tcgcgtcggt cgagttcggc gatcgcggcg cgccgatgtt
     7261 tggcctcgcc gtgttgcctg ctgagggcga aaccttccag gacgcgacca gtgcgacgcg
     7321 caagggcatc gagcagctgc cagcgctgta tgtcgaagag gacgagaaga gcgacgtcct
     7381 gttcgatcgc atgcgcgaag gcgcggttta cgcgctctac tcggggctgg aggacggcag
     7441 cgatcgcgac agccggctag cggcctacgt gctcgccggc cgcgatccga accgtgcatt
     7501 tgcagccgag ctcgacgcgc tgcgcggcat gacgcgagac gaggccgctg cgatcgcaac
     7561 gcagtacttg gcagcaaacg cgccgacggt cctgacgctc aaagcggcca ccgggaagaa
     7621 gagcggccag accctcgcgc tgcacaagcc tattcacgac atcgggccgc gtcgcaagcc
     7681 gcctgatcca gcgctcgcgc gaaagcccgc cgatatcacg gcgcccaacc agatcatcga
     7741 tgccaagacg cgcgtgttgc caaacggcct caacgtcgtc ctgttgccga tgagcagcgt
     7801 gcccaccgtc gacattcgat tgatcttccc caccgggatg gcggacgagg ctcccaatca
     7861 gcgcggtctc gccaatctca cggcgcacgc gctgacctgg gacctgcatc acttcaacga
     7921 cgtcctacta ttcgcggcgg ccggcggtct gaaggacgtt gacgtcgggg tcgaccgcac
     7981 gacgttctcg gtccagggca tggatacgaa gctcgacgtg ttgctcgccg gacttcgccg
     8041 cttggttcgc gaaggcacct ttgacgacga ttctgcagag ctcgtctcga cgatgcgcgg
     8101 cgcggccaag cgtgccgacg acgaaggcga gctgaccgat acgtggcgag cggcggtttt
     8161 cggcgccgat catccctacg tgcgcgccgg cattgcgcgc tattcgaata gcgcattgac
     8221 gctcgacgat gccgcccagt tccgcgcgat ccactacacg cccacgaacg cgacgctcgt
     8281 gatcgcgggc agcttcgatc cagcactcgc cgatcgctgg atcgacttct tgttcgctga
     8341 ctggcgcggc cctcaggtgt atcgacaaac gccacgcagt aagcccgcac ccgcatcgat
     8401 cggcaaggcc gacgacctca cgatgctaca gctgcgtatc gcgattcccg cgatcgctgg
     8461 cggtcggccg gaacagctgg tcgccgccga gatgctcggg gagatcgcgc gcgacatacg
     8521 gttcgagctt gccgccggct atagcgtcaa cgcccagctc tccgagaggc ggttggctcg
     8581 caactacctg ctcggcggct acgtcgatat cgcgcgatcg agcgccgcga tcgagctgat
     8641 ccgcgatcgc gttacccagc tgcgcaacga caacgacgcg gccgcgcgtg cgttcgtcac
     8701 cgcccgcaag cacgtcatcg cgcagctgtt gtcacgcgta ggcagcgcgg ggtcgctcgc
     8761 gcagcgcgtc gagcacgacg tcgagatgga acgcgatccg atgtccgatt tgaagaccgc
     8821 cgcaacggtc cgtgcactga cgctcgacac catggccgca acgctcgccg atatcgacct
     8881 atcgcgcgcg accgtgatga tgcgaggccc ctccgcagag ctcaaacaag cgttcgacat
     8941 tctcggtcgc aaggcgagct acgtgctcga ggacccgttc aaggcgcagg cagctgatga
     9001 ctcggcgccg ggcgcgaccg gtgttctcgg cacgattgcg atgccagtca gaaagtccga
     9061 cgtcgagcct gcgctcacgc tgcaaccgcc gccgcgactc gcgctcacga tcgcggtcaa
     9121 cgcgctcgct ggattgctct tcgaaggatc ggagttctac ccggcgttgg gagagtcagt
     9181 acaggtgcac gccggctatc gctacatgcc cgaccgctcg atcggtgcgc gcctggcagt
     9241 aggcaagttc gccggcagct acggagccgg tgagcccggc ctcgatttca cggccgtgcc
     9301 gatcgatatc ggcgcggtca ttggcttcga cttcagcaag aagcgctcgt acctcgaagc
     9361 gaacgtcggt cttcgtctcg atcgcgtcag cgacagcatg ccgacgcgca catacaaagg
     9421 gctcggttac ggcgccatcc tcgggcgcaa cctcgcgcgg atcggtggca gctggctcgc
     9481 gctcgcgttc ggcttcaatg cgatgagcta tcggacctcg gactcgggcg ccacgctgct
     9541 gacggtaggt ctcgttctgc gacgctgagc gaaaatgtac ccacttgggc gagctgcccc
     9601 gttcaagggg catgcgcctt ccgtcagtcg ttttcgcgct cttactcgtc gcctgccaag
     9661 gaggtaccga tggcccggag gatccgggcg gtgggaccgc acctgatgca ggcactacaa
     9721 cgcatccacc cgatgcaggc atgacgtgcg tccccaagac gtgcgcgcag cgcggggccg
     9781 agtgcggcac caccgacgac ggctgtggcc acagtctcga ctgtggttac tgcctgtttc
     9841 aaagcgatgc gtgcgagagc aaccagtgcg tctgccaacc gtcgtgttac atgcgtcagt
     9901 gcggtgacga cgggtgcggc ggcacgtgcg gatcctgtgg cgcgaacgag tcgtgcaacc
     9961 agtacggcaa gtgcatctac gaaggcgccg gcacgacgcc acccgcgacc tggcactgca
    10021 atgccgcgta ctgggatgcc ggcgatggct gcgactgcaa ctgcggcgca ctcgatcccg
    10081 attgcggtca gtcgggccag tcattgcatg gctgcaccgg actgaacagc cccacttgcg
    10141 atgcgcaagg tctgtgcacg ggcggcggcg cgtgccactc cgttccaggt agcgcgtacc
    10201 tcacgcacta catggtgatc cagtcagcgc cgacgatgta tggcggcacc gtcgcatcgg
    10261 gccgatacga aggcacggac atgcacagct acaatccgcc cggcggaatt tcgggctacg
    10321 ccggcggcga tggcgtcgcg atcgaaatca acggcacgac gtggaagcgc atgcggcgcc
    10381 ccgttggcgg ctacccgccc gtcgacgaga cgttcaccgc aagcttctcg ggcactacgg
    10441 tcacgctcac gcgcacctgc ccgtcggcgc tcaccgagac gttcacgtat acggcgtcct
    10501 cgacgcagct cgtgctctcg cagccgagcg gctcgggcac gaaggtcatc acgttcacga
    10561 agcgctggtg acagaccaca cgtgatgtcg gagcgccgcc cgatcagggt gggcagacgt
    10621 tgaagctgcc gatgcgtacc gtcgagggcg ccgcgacggg ctgactcgtc agcgtcatga
    10681 acgacgggag gatcatctcg ggtgggggcc gggtgtgatc ggcgccgatc ctcacccagc
    10741 gctcgccgtc ggcagacact tcaccgatca cggcatcctc gtcgaggcga acgcgccacc
    10801 acacggctgc cgggtcccag ggcgcgtagc cgaaccgcgt cctctcgtcg cccatgatgt
    10861 gctcgaagaa cagcatgtcc atggccgcgt cgtacccaac gcgccatgcc tcgacggaag
    10921 ccaacgcaac gagaaccgag tcctcaccga cctcggtcgc gacatcgtgc acttctgcga
    10981 tcgtcccgaa cgtcgaagtg aacgggatca gctgcgtcgc gccgcacacg caaaacacgt
    11041 cacccgcacc gggcttcggc gccatcacga ggtcgccgtt ctcgacggtg gcggtgcagt
    11101 cgagctgcga cagctgcccc cacgagcacg gcgatgccgt cggctgctcg aagtcgtcgt
    11161 gtggtgccga ctcgagttgc acacagggcg tcgagtcgac gtcgggtgtg gagctgcagc
    11221 cgcagaccat tgctgcgatg acccgactaa aggcggtgag ccgttggtac ttcctcacct
    11281 gcggagtcta tctcgacgtg tcacgccccg ggaatcagca ccgagcccac ggtgtgcggt
    11341 gtttcggcga agtcgacgtc gagcgtgagc tggaattcgc cggtggtcgg gttgtcgacg
    11401 aggatctgga acatcccaga gtcggccgac ttcgagtagc tggtgccgac gtgatagagc
    11461 catacctgga cgatggccca cttcggcacg ccgttttcgt agatgaacgg accgagaaaa
    11521 acgaagcgct cggggccacc cgtcatcgag cactgatcgt aggtcgatgg cacgtcggcc
    11581 acgcacaccc agttgagtgg atcggtcgag ctcgctcgct tgatcgacgc gtactgcagc
    11641 gtgaacggca tcccgtacat gacgcccgtc acccgcgtct cggtgacgcc agcatctcgg
    11701 cgtgcatcgc cgttatcggt gccgtccgca gcatcggaca tttccccctc gctgccgccg
    11761 caaccgattg ccaacaccaa gagaaccaac ccgcgcatca cgtcattatt tgctgagaac
    11821 gagccgaccg cgaggtgagc attgtcgtac ctcacgattc ttgctggtgg atgtcagctc
    11881 ttcgtgatgg tcagcaccat ctcgatcgtg acatccgtgc cgacctgctc ctcgggattc
    11941 tgagcggggt cgctgccgat accgaaatcg agtcgcccga acgtgtgtgt gcctttgatc
    12001 gtgatgcgat ccgctttgcg atcgaggacg tcgaacgtga tcggataggt cttggtgatg
    12061 ccgtgcgcga ccacagtcgc gtcggccgcg tacgtcgcgc cggctttctg cttcacgttc
    12121 gcgatcgtga tcgtcgcggt cgcgagcttc gcgacatcga ggtaggccgg cgacttcaga
    12181 tgttcgtctc gctcatccga gtcggtgcga aagctcgaga gggcgatctc gatcgtcgct
    12241 gtgccgccct cgatcttttg cggatcgaaa tcggccttca cgacgcggaa cttctcgaag
    12301 ttgatccgaa ccggatcgct cggcttggga ttcttgtgat gagcgagcac ggtgatgtga
    12361 tccgcgcgag cgtcgggctc gctcgcgggc tcagtcacgc tcgcactcga gcccgcggac
    12421 ccggtcgctg gcgtcggcgc tttgtcttct tccttcttgc acgccggcgc agcgagaccg
    12481 gcgacaagac aggcagcagt gaacaggttg gctaacttca ttggaggctc cgcgccggtc
    12541 atcaatcagt cgtgtggcgc agcacgagtg acacgtgaat gggaatgcgc tcgccgtcga
    12601 gatcgtgtgc cttcggtgcg agcgcggtat cgcgggtgac gagcgcgaaa tcggcctgca
    12661 cgagcagcac gtccccggcg agaccgagac gggccagcgg tgcggcgtcg agcttcgtga
    12721 gcgtgccggt gacgtcgatc gcgtgagtcc ggcccatcag cgagacggtg gctggcgcgc
    12781 gaaacgcaag ctcgcgtggg ccgttcgcac ggaccgccgc ggcgtgatcg agtgtgacgc
    12841 cgatgcgcgg gaactcgtcg gcatggagat agccctcgcg cgcgtgcttg ctgcgttcgc
    12901 cgacaccggt atcgaccgag gccgctgcaa cgctaatctc ggcatgcagc ggcctgcgca
    12961 gatcgcgagg cgtggcccgc caggtaccgg tgacgtcgcc aaacctcgcg tcgaacgctt
    13021 cgccgcctgc cgacaggtgt gcggtgaccg cggtccccgc atgagtcacg acgacgtagc
    13081 taccttccgg tgcatcgagc gagccctcga tgccgtcttc gatcaacacg gccgccgttg
    13141 cgggcaacgt gtcgcgcgca tggaggggcc ctgccccgct gacgaacgcc gctgccggaa
    13201 cgcagagcga tatcgccgcg agacaccacg acccgacacg caggagccga cctcgcgctg
    13261 gttgcgaagt gcgccggcgg atccaccagc cgacaacggc gagcacgagc atcagcccgg
    13321 cgatcgcggc gcagacgtcg agaccgagcc agatcggatg ctgcgtgctg atgcgccagc
    13381 cgagatcggg atctggcacg tagaagcgct tggcgagcgc cgtgtagacg ttgcccgagc
    13441 cctgcacata ccaacgcacc agcgacgcgc ctaatgccgc ggccacgacg agcaccggcg
    13501 caaacggcga ccacgagtgc aggattcggc gcatcggtta cgacccgctt agtagagcta
    13561 ctacaactgt agtcattgag caacgacgag atttcgtgcg actgcgcggc gcgtcgaggc
    13621 tcctccttct atgagccagt cgcgcgggcg tcgcgcacgc gctatcacga tgcccaagat
    13681 gaagccggtg cgtccgatcg cgaagccgac cgcgcccccc agtgacgact ggcatccgcg
    13741 caccatcatc atcgtcgccg cggtcaccgt gttcgtgatc agtgcgtggg tgccgtttgg
    13801 ttcgaccgcg ctctatccgc tcacgctgtt cacgacgtgg gtccatgaaa tggggcacgg
    13861 tctcaccgcg ctcgcattcg gcggcgagtt ccgctcgctc gagatcaacg cgaacgcggg
    13921 cggcactgcg tatgcgttcg cgggccacgg ctggcccgac gcgatggttg ccgcgggcgg
    13981 ccttctcgca ccaccgttcc tcggcgcgct actgctcgcg ttcgtgcatc ggccgcgcgc
    14041 tgcgcgcatc gtgctcggtg tgctggctgc agcgctcgtg ctgtcgctcg cgatctgggt
    14101 gcgctcggcc gcgggcatca tcgcgatgcc gctcgtcgcc gccctgctcg gctgggccgc
    14161 gtggcgtggc ttcgccgaga atcccggccg ccgcgtcgtg ctcgtgcaag tgctgtccgt
    14221 gatcctcggg ctcgacaccc tgacgcgaat ggtcggctac gcgttcatgg acaagacgtc
    14281 gttaggccag cgctccgatg tctcggcgat cgccgataat gtcggcgggc attacctgct
    14341 gtggggcctc gtgatcacga cgctcgcgct cggcatgctc gcccttggcc tgtggcgcgc
    14401 ctggcggcgg cccgcgccgt aaattgcctg caacagcagg cggtgcgccg tgtcatagct
    14461 aggtggtgca gcgagtgctc gcgatcgtct gcgcgctgag cgcggtggcg cttgcggact
    14521 cgtatcccat gcgcagcacg cgcaagctcg aggtcggcat ccacgagggc gtgccatggc
    14581 cgctcgccaa tgcgccgatc gacggacgat tgccggatgc accggtgtcg cgcgacgcgc
    14641 agccggatgc gccaacgaag gctggcaaac gcgtgctcga agtgatcgac ggtatcgagg
    14701 agtcgctgcg cgacacgtca tatcaagcgc gcacgagcgt gcgtgccgac aaaggcgagt
    14761 accactggga ctgctcgggc atggccgcgt ggatcctgcg acgcgcggca ccggcggcgc
    14821 tgcgcaagct gagctcggaa cggccggtgg cgcgtgactt cgcggatgcg atcgagcatg
    14881 cgccggcgaa acgcgcgaag gatggctggc agcggatcgc gaacatcgcg gacgtgatgc
    14941 ccggcgacgt gtttgcgtgg cgacggccgc gcggcatgcc gagcaagaac accgggcatg
    15001 tcgggtttgt tgtcgagcga ccgttgcgcg tgcccgagat tccaggcgcg tgggcggtgc
    15061 gaatcgccga ctcgacacgc ggctggcatc agaacgatac gcgcaccgat cacgacggcg
    15121 gcggattcgg catcggcacg ctgctgtttc tcgccgacgc ggatggcaac gtcacgcagt
    15181 acggctggag cggcacgtgg agcgagtggt acgtcgtcac gcacgtcgtg ttcggccggc
    15241 tgcatcgtta gcaattgcag gcgtgtgcag gcgcacgtgg acgcacacgt tcgcaccgat
    15301 ggcagcgacc atctggtgat agcatctaca tgatggggct ggagcggctg agcctggcgt
    15361 gcatcgtgct cgtcggctgc tattcgcccg agttgcggcc cggatcaccc tgctccaccg
    15421 actcgcagtg cccgtcatcg ctgcgttgcg tcgcgggcgt gtgcggaggc agcgccgtgg
    15481 atttcgatgc acctagcggc ggtggtagcg acgtcggcat cgacatcgac gcgccactcg
    15541 gcgatcccga tcccgatggc gatggaatcg tcggcgccgc ggacaactgc ccgaacgtcg
    15601 ccaatcccga ccaaggcaac gaggatggcg acaagttcgg cgacgcgtgc gatccgtgcc
    15661 cgatcgaggc gaacgattcg ccaaccgatc ccgacggcga tggcgtcgcc gacagctgcg
    15721 atccgatgcc gaacatggcc ggcaataaga tcgtgctgtt cgaaggcttt cacaacgcgc
    15781 tgccagcggg ctggatgctc gcaagcgcga cgaaggtcgg cgacgacgtc aggatcgtcg
    15841 cgagcggcac caatcactcg ttcatctcgc cgccgctcga tgcgacgacg aaggtgacga
    15901 tcatgacgcg cgttgttgtc gaacaaacca ccggcaacaa cgacgctgac tttggcgtct
    15961 cggttcctta caacccggcc aacgacgaca gcattcgctg cgagctctac tcacccactg
    16021 cacagcagac ggcgaatcgt gatctctcga tctgggacga aatccgctcc acggagatcg
    16081 gaaatcagtc gtttgcgtgg gtaactggaa cgccttatct cctcagcctg caacgcgccg
    16141 acaccaccta tacgtgtcgc gcgtcggcgg taaacggcac gccggtcatg gcgtcgggca
    16201 cgtcgtcagc ggtgccatcg cagcagaagt tcgcgcttcg cacgtatgca acgtccagcc
    16261 tgatctcgta cgttctcatc gtcgcgaacc cgtagggtgc gtcggtccgc gatgaccccg
    16321 cccaggcgct gattctttgg atcgattcgt tcgcccgcgc gttggcaggt ggaccatggc
    16381 aaggctaccg gtctgcgtcc tggtcctcgt ttgcacgggc ggcaccgccg tcgcgcagta
    16441 cgcccgttat ccgcgacagc aaccggcacc ttcgcccgtt gtgctctccg agcgggtaac
    16501 gcccatcgtg gccaagccaa ccgctcaggt cgagaccaag ccgtcgatcg atgccgatgc
    16561 cgtgttgtca attgaagacc tgcgcagccc gattcgcacc gagcaagaac agattctcgt
    16621 cgacttgatc gcgaacactg cggattccga ggtcgacgaa aaaaccgact actacttccg
    16681 actcggcgag ctctatgcga agcagcaccg cttctggcgc atgaagagtg tcgagctcgc
    16741 cacggcgaca gacgcgaccc gcaagaagca atccgcgcag gctgccgatc aagccaagac
    16801 gtatttactc aaggccgtga agacgttcaa aggtctcacc gacaacgacg cgtttcgcaa
    16861 ctatcccaag ctggatacgg cgctcttcta ctacgcgtac acgctgcagg gcggcaagta
    16921 catgaaggag gcgcgacagg tctacgacaa gctgctcaag aactatccga gctcgaagtt
    16981 cgtgccggaa gcgcacctcg cattcggcga ctactactac gaggccggcc agctcgcgga
    17041 cgccgaggca cgctacaagc aggtcctcaa gttcccgaag tcgacggtct acgcctacgc
    17101 gctctacaag ctcggctgga tccacctcca actgtcgcga ccccaggagg cgaccgaagc
    17161 gttctatcaa gtcgtcgccg caacgcgggg cgacgcgagg caagccagcc tcaaccacgc
    17221 ggcgaaaacg gacttcgcgc gcgcgtacgc cggcatcggc aaggcggagc acgccttcga
    17281 cgcatttcag aagctcgata agtcggcagc cgtcgagctc gccgagctcc ttgctgacat
    17341 ctcccgcgac aagggcgact cgaaccgcgc ggtgactgcc taccgcgacc tcttgctgcg
    17401 cgcccctggc gacgcccgcg cgtgccgatg gcagtaccga atcgcgcacg cgacgctgtc
    17461 gattccgggc gcgacgaacg ccgtcaaagt caaagaagtt gaagcgctcg tgcacagcta
    17521 caccagccag aaacagccgg atgccgaatg ccgcgcgaat gcggcagcga tgtcggccga
    17581 gctcgcggct gcgtatcacg cggagtggtc gaagacccag aacgtcgaga cgctcggcta
    17641 tgcggagcag ctgtacgcga tgcacgtcgc cgcgttcccc gacgctgcgg cacagcgcga
    17701 gacctatgcc gaggtgttgt ggtcacgcgc cgatcacgag agcactgcga aggttcgtgc
    17761 cgagcgctgg cagcgtacgg cggacgtgtt tgcaaccatt cacacgcagg aggccgcgcg
    17821 tgcgactgcg ctcgcgtgga tgaacgcgct agacatcacg acgcccgccg acaccaaggt
    17881 cgtgatcggc aagccaccac gctcgcgacc acgcgcgctc ccgctcaagg gactcgaggc
    17941 aaaattggtc gccgcagtga ccgcgtacac ccagcagtca cccgacgccg atgaggacct
    18001 cgcacagatg cgcctcgcga ccgcgacgat cttgcgccgc aatcgccact tcgatgaagc
    18061 cgcggcgatg ctcgacgagt tcctcgagca tcacccagac cacgcgcttg ccgagctcgc
    18121 cgcgaacctc atgctcgatt cgctggtgaa ggcagccagg ctcgacgacg cgcttcaggt
    18181 cgccgatgcg atcgctacgg accgtgcgtt cgtcgaaggc aagcaagagc tgcagcgcaa
    18241 catccagctc ctgcgcctac ttcattaaat gtgaccgcca ggtttcgcgt gtaggtcgca
    18301 tctttgggag cccgggaggt gcatgatcgg tcctgcgtgg agaaggaggc cgacactcga
    18361 cccgcgggat catcctctgg tggagggaag agcaaccgtg agttctccgg aaccgatcgc
    18421 tttcagctga ttcgccagct cggcatgggc ggcatgggcg tcgtgtacga ggcgctcgac
    18481 cgcgagagcg gcgagaaggt cgcgctcaag accctgcgca atgtcgatgg caacgcgatc
    18541 ttgctgctca agcaggagtt ccgggcgctc gctggcgtcc atcacccgaa tctgatccga
    18601 ctcggcgagc tgttcgagtc gcgcggcgac tggttcttca cgatggagct gatcagaggt
    18661 gtcgacctgc tctcgtgggt gcgagacggc gattcgcagc ggttgcgcga cacactcgct
    18721 cagctggtca ccgggctcgg tgcgctacac gacgcaggtc acatccatcg cgatatcaag
    18781 ccatcgaacg tcctcgtcag cgacggccgg gtgatcctcc tcgacttcgg gctcgcaact
    18841 cgtcacgacc acgaccagct gtcgtggagc gacggcgcga tggtcggcac gcccgactac
    18901 atggcgcccg agcaagcaag cgcgcggccg gcgggccccg aggccgactt ctacgctgtc
    18961 ggcgtgatcc tgtacgaggc acttaccggt cggctgccga tccaaggcca cgcactcgag
    19021 gtgctgatgg agaaacagcg catcgtgcca gctgcgccgt cgacgttgac gtcggttccc
    19081 gcagacctcg acgcgctgtg ctgcgagctg ttgcagatcg atccagcgac acggccgtcc
    19141 cgcaacgaga tcatgcgccg gctcgctgcg acgccgcgcg ctgactcgcc gacggcactc
    19201 acgcgcgacg ccgcaacgat gacaccgatg ccgcgccaga catttgtcgg ccgcgagacc
    19261 gagctcggtg tattgcgcga tgcacttgcc gagtgcgaca ccggcgtccc cgcaaccctg
    19321 ttgatcgagg cggagtcggg aatgggcaag agtgccctcg tcgagcattt cgtcgccggc
    19381 ctcgaccgcc gcgacgtggt ggtcttgcgc ggtcgctgtt acgaaaacga gtcggtgccg
    19441 tacaagggcg tcgatggcgt gatcgattcg atcagtcggt tcatggcgcg cctgcccgac
    19501 aaagaggcgg cagcgctact gccgttccac atcgggcttc tcgccgaggt gttcccggtc
    19561 ctgcgcggcg ttagcgtgat cgtcgaacag cctcccgtac aacacgtgcc ggatccacag
    19621 gagcagcggc gccgcgtctt cgcagccgtg cgcgagctac tcgctcgcat tggtgcgcgc
    19681 cacccgctcg tgatcatgat cgacgacctg cactgggccg acgaggacag cctcgccctc
    19741 ctcgacgcgg tactacgtcc acccgacgca ccgtgcctcc tgctggtcag caccgcctgg
    19801 ccacgcggcg acgagccgcc gattcagttc ggctcgaacc tacgccgcat cgtgctcgaa
    19861 ccgctcgacg agcaggagac cacgcggctg gcgcacgcgc tattcacggc ggtgcgcgca
    19921 ccgatcgccg aagcgacgac cgctacgatc gtgcgcgagt ccgcaggaca cccgatgttc
    19981 gtcgccgagc tggtccgcca cacggtgctg cgcggtgaca ccggcacgac ggccagcgag
    20041 attcgcctcg acgacgtgct cgccgagcgc atcgatgcac tcgagaccgg cgagcgtcag
    20101 ctgctggagc tgatcgcggt cgctggccag ccgcttccgc tcgaggttgc cggccgcgcg
    20161 ctcggactga ccccgtcgaa gctgttgccc ttgatcggct acctgcagtc ggcgaacctg
    20221 ttgcgcgcga ccggtcgcac gaggcgtgac tcggtcgagc cgtttcatga ccgcacgcgg
    20281 cgcgcggtaa ctgcgcgatc cgcagaacgc aagcccgacc tacaccgccg gctcgcagtt
    20341 gcgctcgagg cgaccgatgc ggagacgcag ccgatgctcg tcggtgtcca ctgggaacag
    20401 gccggcgaac cggcgcgtgc cgctgcgctc tatgcacgag ccggcgatcg cgcgactgaa
    20461 gcgctcgcct tcgagcgcgc ggtcaagctc tacgagcgct gtctcgcgct tgccccggag
    20521 ctgtcgccgg tgttgcgcgc caagctcggc gacgcatatg caaatgcagg ccgcggccgc
    20581 gaagccgccg aggtctatct cgcactcgcg cgcgagtcga agatcgccgc cgacgcgctc
    20641 gagctcgagc agaaagccgc acatcagctg cttcgcggcg gccacatcga cgacggcatg
    20701 gtggtcctgg agcgtgtgct cgcatcggtc gacatcgact taccgaagac gccacgccga
    20761 gcactcgcat cgctgctatg gcgtcgcgct cgcatgcgac tgcgcggcac cagcttcaag
    20821 cctcgcgatt cgagccaagt gacgcgcgag gagctggcgc ggatcgacat cgtgtggtca
    20881 gcggcgtgcg ggttgtcgat gacggactgg atccgcggtg catcgtttca ggcgctcaat
    20941 ctgctgcttt cgctcaacgc cggcgaaaca gagcgtgcgc gccgcgcgct gttcctcgag
    21001 gcgtgtcacg tcgcggcagg cggtgcacca gaccgcgccg agacgctcgt caaagctgcg
    21061 ctcgcgggcg gcgaccccga cgatccctac ctgcgcggct ggctcgagct cgcgcgcgcg
    21121 tacatcgcgt attttcgagg cagctggcgc aaggcgttcg agggcgccga acgagccgat
    21181 gcgttgttca gcgccaactg cgcgaacgtc gtgtgggagc gcgacaccgt gcacgcgctc
    21241 agtcactggt cgcaggtcta cctgggctcg ctcaagacgc tcggccagat gctgccggcg
    21301 cgccttcacg aggctcagct gcagggcaac ctctatgcgg tatgggcact cgcgtcgacg
    21361 gtctcggtgc ttcattggat cgcgcgtgac gacgttgccg gcggtcgtgc ggccgtcgat
    21421 cgcatctccg aacgctggtc gctgcgcggc taccaaatcc agcactggaa cgagatgcaa
    21481 tcgaatgcgc tcctcgacat ctacgccggc gacgtgcgcg aggcgcgacg gcggctcgag
    21541 gacggctggg tgccgatgtc gcgctcgcta ttgacgaaga tccaggtcat ccgcttcgag
    21601 gtcaacgagc tgcacgtgcg gacggcactc gctgcggcgc gcgtcagtag cggcagcgag
    21661 cgcgagcaac tcttgaagat cgccgagcac cataccgata agctcgctcg cgaagggctc
    21721 gcgtggaccg atgcgatcac gacgctacgg cgcgctggca ttcgcaacgc acgcgaagac
    21781 gccgacggcg ccgtgcgcga atatcgcgcg gcagtgaccg cttgcgatgc ggcggagctc
    21841 ggcatgcatg ccgcggcggc gcgcgttcgg ctcggtgcgc tgctcggcgg cgacgaaggc
    21901 ggcgcgctgc gcgaagccgg ccttgccaac ctgcgcgcgg aagaggtcaa acaacccgac
    21961 gccttcgtcg cgttgttcgc gccgtgatca tcagccgccg cgcagaccgc gcgcgaactg
    22021 actcaccggg tagaggatcg cgcgtacacc gtttcggaac ccggttggca cgacgccgcg
    22081 ccccgtccgg aacatcgaga tctggaacgt cggatcgtca tcgggcagac gctcgatcag
    22141 gcgcagctcg gcgagtcgac ttcgcaccgg accgtcgggg cccggcgcct cgcgcgcttc
    22201 gaggatgaac ttcgcgcgcc ccgctgcgat gtccgcgtcg agacgttcgg tgcgcgtgcc
    22261 ggtcttcggc gcgctcgcgg ctggatccgg gatgagacga aaccagatca cgccgagccc
    22321 gcgagctcgc cacggcgcga ccgagccgta ttggttggca aggtagtcgg caacgttggt
    22381 gtgcttggtt gcgtcctcga gatggagaaa cgattcgaac gtgccggcag cgagatcctg
    22441 atcgctgccg agcttgatcc ccatgccgat gatgctcggt gacggcgagt tctcctccgc
    22501 cgtcgagctt gccatgcgta tgcgcgcagg ccccgcgagc cgcgggccga cgacatcgtc
    22561 gagcgcggtg acttcgacgc ggcacacgct gccatgcgga tggaacgctc gcccaccgcg
    22621 acgcaggcta cgaaagaaga tccagatgct cgcgatgagc gcgaccagca caccgagacc
    22681 gaacagcagg gcaagaccga tcgagcgcaa gatattcacg ggtgcctcga aatgaaccgc
    22741 agcgccgcga tgccgggcag aaagaagtag ccaccgccga cgagccgcgt gaacggcggc
    22801 atcgccttgt acttccggcg caccggatcg gccgggcacg tgaattcgtc gttcgggttc
    22861 tcgtcagcgc tcgaacgccg tgcggcagtg atcggatcgg catccttgaa cagcgagtcg
    22921 aagttcgccg agtggaccca gtttcgctgc acgaactcga actgccgttc gatgcttccg
    22981 acgagacaga tgaagtgaag accacgccgc tcgtgatccg gtttgccacg cgccgccagg
    23041 atgtcgcgtg gattcatcgt ttcagcgacg ggacggccga acgcacggcc acgccggatc
    23101 atctggtgct tgcggaccat ctgcttcgat gcgtcatgac tacgatcctc caccgcaagc
    23161 acgtcgcgcg gattcgaccg ccggatgtgc gcaccgtgtg ggcagcgcag tcccgattcg
    23221 tcaccttcgt agacgaaatc gttctcgtcg gcacgcttgt catcatcgcg ctcgggcgag
    23281 gtgatcagcg gcgcgccgcc gggccagcgg ccgactatct tggcaccgag tgcgatcgct
    23341 cgcgccgctg gatcctttcc gggttcacgc gagctcttct cgaggtagtc ccagagctcg
    23401 atcacgtgct gtgtcatctc gcggtagacg aggtaggtgc cgttgcggcc cagactcttc
    23461 ttcgtttcgt tcccgacgac gatcaccggc aggtggtttg caggatcgtc ggcgagctcc
    23521 gccgtcgggc actcggtgaa cgcctggtag tcgttgcgat agccgagcac gaattcgccc
    23581 gggggaagtg ggtcggtcca cgactccttg cgcttcttct gcggccgctc tgccggcgcc
    23641 acagccggca ccgctggcgg cacccctgca atcttcggca tcgagatgcc atcgcgccac
    23701 ccgaagtgct ctttctggcc ggtgagcgtg atcgtctcct tctcgtgccg gatcttgaag
    23761 ccggcctcga tggacggtcg ctcggtctcg agcacctgcg tcagtgtggt gtcgtcgagt
    23821 gcgtacacca tgagcagcac gtggatcggt tccggctgcc gtccccactc ccacgtgctc
    23881 ggatcgttgg cgccgcgatc gcccaggatg tccgggcgca cgttgtcatc catgccttct
    23941 cggaactcgc gcgagaaggt gtcgagcgcg ctcttcggta cgccgaggcg tgcgagtcct
    24001 tgggccgtga atgcaacctg gagcgccgtt gtctcgggcg aaatgctcgc gaggttgagg
    24061 cgcccgcaca acttctgcaa gtagtcgcgc gcgcggtcat cgttcacgac ctcgagcaag
    24121 aagaagcgcg ccgcggcgag cggtccatag ccacgcagga ccagaccttg gatgtcctcc
    24181 gcctcgagag tctcctgtgg ttgttcggat gccgtcacag tagctgcagc cacttcttgg
    24241 cttgatcctc gttgtagtcc gcgaacaagc cctcgcgaat ctggcgattg cgcttgatgt
    24301 tgtcgatgct gatgtcgcga tacgggccat accagaacag ggtccgtcgc tggtgttcgc
    24361 tcagtgcata gaggtacgcg ttcggatcgg tgagcgctcc gtcccagagc agccatcgtg
    24421 tcttcgggaa gccacggcca aagccgaacg acaaattgat ccgcatcgca ccgccgcgcg
    24481 tctcgatgaa gtcgcgcacg taggcgatgc cttcgttcgt gtagttggag atgaacatca
    24541 gccggcggcc gccgtcagcg gcgatccacc gcgcggtcgc caccgtcgga atgttgatgc
    24601 cactacccac gtacggcatc cacggcacgc cctccacgac ccgcgcgatg acccacagtg
    24661 acaccttcac gaatgcgggt cgaagcatgc cctcctcttt gatcgggccg gcgagcgtga
    24721 actcgttgat caccggacgc gtctgcgtcg cagccaagaa tcgcgcacgg tcatccggcg
    24781 ttcgtgcggc gacgtacgtc tgatcgtgct ccgcctcgaa gatcgagagc cacatgacag
    24841 cgacaaacac gacaaacgcg aggaccgcga tccagccgca cgctgcaatc acgccgaaca
    24901 gcgagctacc gccgaaccac gcgccggtgc agaagatcag cgccgcaaga aacgcttcga
    24961 tgatcaacga gcgccagtga aacaccagcc acgcgcgcgt cgactcctcg accggctgtt
    25021 ctgcccacgc gaggtcggga cgcgacagca cgaacttctg gatctcgttg cgcaccacca
    25081 gcggggagcc gttgaaacca ccgtgcgctt cgcgatcgtc gatgaactcc tcgatcgcct
    25141 cgcgcagctg tcggtgctgc tgcacatcgg cagggctgag gccctgcatg cccgaataga
    25201 acgtgtcccc atggcggtga tagatgagga agtcttcgag ctcgtcgtcg gagcaactgg
    25261 gctcgaaccg ttcgcagtgt tgcagcacct cgcggaggcc atcgcccatg atgcgaacga
    25321 gctcgctcac gtgcacgcgc gtcgggccgc agaagctcgt cgcgaacaag agcatcgccg
    25381 gtaacggctt gtccccgtac ttctgcgcgg ggatgacggt gatcgtcgcg aagtgtgtcg
    25441 tcgcggattt cgcgaacggc agccgctcct gatgcgcatc gagctcgacg agcaacgcgc
    25501 ttgccgcgtc cacgcaatcc gtcttgagcc ggacgaggac ggtgagtcca gcgttgacct
    25561 tgtgcatccc ctcctcgcga gcgatccgcc gcacgataac gcagaacggc cggactccga
    25621 aattgcccga gatcggctcg cgcggttgtc gccgcggacg acgcgctcac gctagggtct
    25681 tggcgtggac ttcgtcgacg ccaacgggat tcggttcgcc tatctcagcg agggcagcgg
    25741 gccgctcgtc ctgctactcc acggcttccc cgatacgccc tacacgtggg agcgcgtgat
    25801 gcccgaggtc gcgcgcgccg gctatcgcgc agtcgcgccg ttcatgcgcg gttaccatcc
    25861 gacgtcgatc cccaaggatg gcaagtacga cggcgacacg ctcggccgcg acgtactcgc
    25921 gctgatcgaa gcgctcggcg agcagcaggc gatcgtcgtc gggcacgatt ggggcgcgct
    25981 cgcggcgtac accgcggcgg cagtcgggcc ggagcgcgtg cgcgcgttga tcaccgtcgg
    26041 cgttccgcat ccgcgctcgg tactgccgac accgcgggtg ctgtggacgg tgcgccattt
    26101 cttcgtgctg cgaaacaagg gcgcggcgca caagatccgc gcgaagaacc acgcgtacat
    26161 cgatcagctc gtgcgccgat ggtcgcctgc gtggaaagac atgccggcgg acgagaccgc
    26221 tcgcgtcaag caagcgtttg ccgagccagg ctgcctcgaa gcggcgtgca gctattacgc
    26281 tgcgctcaca ccattcatgc ccgcatcact gcgcgcatcg atcaaagtcc cctcggtcgc
    26341 gtttgccggc attcacgaca acatgtcgcc gcgcgtgtac gagaaggcgc ggcgctgctt
    26401 cgtcggatcc tacgaagtcg tgcaggtgcc aggcggccat ttcatgcatc gcgaacatcc
    26461 gaacgaattc accgcggagc tgacgcgggt gctgcgggcc gcaggttagc cgcgtctcgg
    26521 ttgtttcggg cacatgcgca gccgaccgct gcgacaggac gccgcacagc gattgccagg
    26581 gtccgttgca tcctagttcc atgggtaagc cgccatccca gatcttggag ctgggaccgt
    26641 caccgaacat cgaccacgag aaggacatgg ccgagaacgg cgcgccgccg aaggctaccc
    26701 gggcgatcta ctggggcatc atcatcgcgt tggtcgtgct gttcgcgctc gtcggcatcg
    26761 gtctgcatat tccgaccggc ggctgagccg cgcgcacggt cacgacggta ggatcgccgt
    26821 cgtgctttcg catcgaagcg tgctgtgcgt aatgctgggc gcgatcgcct gtggtggctc
    26881 gagcaagcgc cacgaaccga cggcgcccga gctcgtcgca ttcgtcgacg gcaaatccgt
    26941 gttcgggccg gcgaccgaat ggatgaagag cgacctgccc gacgtcggcg aaaccgggct
    27001 gttccccgct cgcacgcccg agcagtgcga agtcgcggtg ctccacagcg gcgaagactc
    27061 gttcgccgcg cgcatggcgt tgctctctcg cgccaagcac tcgatccgga tcgaggcgct
    27121 catcttcact ggtgacgagg ccgggctgcg cgtcgccgaa gtgctgaagc agaagaaggc
    27181 cgccggcgtc gacgtgaagg tgatcgtcga cggcctgagc aactcgtcgc tgcagacgca
    27241 gtggatgtac ttcgatctca agcagcacgg catcgaagtc gaaggctatg aagcgatcgg
    27301 cctccagctg atcaacgaaa tccccaatcc gctcgtcacg ccgtggcacc gcgatccggc
    27361 gcgcccgaac agccgctatc acgagaagct gtggatcgtc gatcccgacg agcccgacgc
    27421 cgaggcggtg atgggcgggc tcaacatcgc gaacgagtat ttccgcgtcg acccgactaa
    27481 cgttcctcgg tactggcgcg accaagacgt catcgtgcgc ggcgtcgtgc ttgccgatct
    27541 gacggcgacg ttcgatcgca acttcgctca cttcaaggac ctcaagcgtg accgcggtgg
    27601 cctcaccgac gccgcgtggg ccgcaacgcg caagatcatg gacaagaccg gtgcaccgac
    27661 gatcgcgttc gagcgacgtg acgagctcgt gcgcacggtc gccgcattcg aggcgcgcac
    27721 gccggacctc gattatcagc ccgcacagtg tcgcttcctg tggtcgcggc cgcgcctgca
    27781 cgaaagctac atccagcagg cctacctcaa gctcctcgcg accgcgcgca aggaagtgct
    27841 gatcggcaac gcgtacttcg tgccgacacc gagcgtgcgc ctcgccatcg agaaagccgc
    27901 gcgccgctgc gttcgcgtct ccctgttatc gaatggcccc gagaccaacg acgagcccgg
    27961 catgaacctc ctgtcgcgtg cgtattacgc cgagctcctc gcggtgaacg cgagccccga
    28021 ggtcacagcg tgcaagcagc ccgctggcgt cgaaatctgg gagtggcaag gcaaagcggc
    28081 cagcgatgcc acgcagacgc agggcctgta tcacgccaag ttcgccgtcg tcgatcgcac
    28141 gctgtcgctc gtcggttcgt acaacctcga tccgcgcagc gagatccaca acagcgaaac
    28201 cgcgatcgtg ttcgagcaac ctgccctcgc cgcccagctc atttcgctct acgagcacga
    28261 cctgaccacg agccgtcgta tcaccgacgc cgaggccgct gggttcgaga agcctgattc
    28321 cgttcagcgc cggatgaaga aggacttcgc cgcgctgttc gaaagccagc tgtaacgcta
    28381 cggtttggct ggggcggtag ccggtgccac cgcaggcgct ggcgtcttcg cgagcacgcc
    28441 gatcacgagc gccacgctgc tcgccccgag tgcgagccac gcaccgaggc cgaagatgag
    28501 ccacgcgctc atgccgatca cgaacaggaa cgcgctgtag accagcgcgc cgcccgcgag
    28561 cgagagcttc g
//
LOCUS       DASUFX010000597        10761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1346905, whole genome shotgun sequence.
ACCESSION   DASUFX010000597 DASUFX010000000
VERSION     DASUFX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346905"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFV99_23970"
     CDS             <1..530
                     /locus_tag="VFV99_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062459.1"
                     /translation="LTGTPEFRIPAGDADHNEHMRITVPPLGDLQQVQLYSVNPHMHM
                     IGTHIATKVERPAARGNDPQNECLANGKWNFDWQRTYIYDAPLDQLPSLKPGDVIDLQ
                     CKWDNTIANPFVQRALKDAGLVAPIDVNLGEQSLDEMCLEIMGFSIDAPPPMTRTVPT
                     MDDLPMALLDTLKRN"
     gene            complement(527..2797)
                     /locus_tag="VFV99_23975"
     CDS             complement(527..2797)
                     /locus_tag="VFV99_23975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062460.1"
                     /translation="MPSRDSDVHTSVERADPLVGTIFDKRFRIEERIATGGFGAIYRA
                     THVKSGHPIALKVLLPSLAQDLGVVARFRREGDTLTALRNPHTITAYELAQAADRTLF
                     IVMELLHGESLFERYKANGPFEWKRMVRIAHQVCESLEEAHAAGIVHRDLKPTNIHLE
                     TKADDVDFVKVLDFGIAKILRGSDFDAADITNAGQMIGTLDYMSPEQMVGAKVTGQSD
                     IYTVGIVMYEMIAGCTPFPEAMTAAQALAAVMKTKPDPLYLRAPVPEELDRIVMRCLQ
                     RDIETRYQTVAELRGDLERLLGGAAPDRARAVDTKPVEKHDEATQFTPPPIGLVDRSR
                     RDDPWAPEATTAAQPSAQLLAEARRPKRPSVEEATQFTPPPEKLIAESRRPKRPSAEE
                     ATQFTPPPEKLLAQMRDARANADDEWSDEDEYAPTTNRRPAGAPPPRSSPGHEGVTKI
                     AHMSEISDVLAQTGDDYDGVSVRPSEERVTEVARLADVGKRIPPRAQGGRDMSDRAMT
                     LPRDEVDRAMTVPRESKSDRDMTLPRTLPPRNEDSGLEFPTPPMPAASPPTVSMRSSA
                     RSASAPPPQSPAVPRTLTPQQPPPVPQAPGIPTPRPLGRYETPVPALVAAPFSQPRPP
                     SNPPPLAQPPASSVPPPLSPMPPRQSAVMPTPYPQAPGAIPTPYPQQYAPPTPVPGPY
                     MQQQYGTQQPGYPQYQQSAPNDWLAPVGAQVPQRTTKSFDMGKMAAREAAIRKLIWII
                     VAVVAIGGGVVLATQL"
     gene            complement(2808..3908)
                     /locus_tag="VFV99_23980"
     CDS             complement(2808..3908)
                     /locus_tag="VFV99_23980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062461.1"
                     /translation="MGLITLARWLGPWADATKAPTVDIVDEKVGDIRVRVFRRRGAKA
                     AEAARDVGDDHRHSAPVATALRPPRGATTFLIAPGLHYAGPDDVRMDRFCRILAASGH
                     HVVAPFVPDYLALTPNARAISDFAKVFDALPAGTKPVVFSISFGSLLAFALAAERGDA
                     IDKLVIFGGYADFHETMKFCLTGEVSTGRQATRDPLNQPVVLMNLLEHIDHHQHTRDE
                     LVAGWRSYVERTWGRPEMKARDRFVAVAHELAPSIPDAVRELFLIGIGAQPGAWDLAV
                     PALAQFDATALDPTPYLSRITNRVELVHGMDDDVIPFEQSQVLASRLVNARSVRVHIT
                     GLYGHTGSQRPPLTALGKELVTMIRVLRVLSA"
     gene            complement(3963..5291)
                     /locus_tag="VFV99_23985"
     CDS             complement(3963..5291)
                     /locus_tag="VFV99_23985"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5062462.1"
                     /translation="MSTAPPPVAPAVGRTQTGTDVSLKDRGNLFEVATRQIDKAMKLI
                     NIDSDIKEILGQPKNELIVNFPVRRDNGKIEMLKGYRVQHNNILGPYKGGMRYHHEAN
                     LDEMKALASWMTYKSALHEIPFGGGKGGVKFDPRQYSKVELERITRRFTAALGSNIGP
                     DFDIPAPDVNTNSQTMVWMMDTYMNIVGASDRNTVRGVVTGKSVTAGGSHGRAEATGQ
                     GVVHCITEWAKDKNFNLDGAHVIIQGFGNVGSYAARLLSQKGAVIIGVGDVGGYIANT
                     EGINPHKLGEHVHKTGSVSGYKGASKITREEFFSLDAHIFVPAALELEVGVVEAKALK
                     VKMVVEGANGPTYPEAEEILLDKGIDVIPDILCNSGGVIVSYYEWLQNKRAEQWELAD
                     VLGRLERRMKQTYANVRQYAATQKTDWRTASYAIGLERLQHCYKERGIFP"
     gene            5384..6694
                     /locus_tag="VFV99_23990"
     CDS             5384..6694
                     /locus_tag="VFV99_23990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5062463.1"
                     /translation="MNDHQLAAQVSEGGRLRALVLELWQRTRMDWGFVTDRLATTFRK
                     ETWVGAHERRFVSETLYGMVRHLRRIDLALERGRRVKKPPRDLERLLALLVLDKLLEV
                     DRAARIEPDLDWRAIASVDDVIAGERKLAKRIALAASLPDWLAETLVADWGDDAEPLA
                     LALNQRAPMTVRANLLVGDRAALAAELTKEGLKTHAGAWSDTALIIDSRTNLFASAAF
                     KRGAMEAQDEGSQLLADLAVPTATKSLVIDYCAGAGGKTLAIAARLANRGRIVASDVD
                     GKKLEELRRRARRGGVSNAQAIAIEGGKWPRELDALRAKADVVFVDAPCSGIGALRRN
                     PEARWRMRQTDIAAFAARQREIMAAALDLAAPGARVVYATCSLLRAENEDVAQSIAGT
                     GTCELLPLSDVLGDRANVFGGEAFTVAPHTHGTDGFYARIMRRL"
     gene            6902..8626
                     /locus_tag="VFV99_23995"
     CDS             6902..8626
                     /locus_tag="VFV99_23995"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5062464.1"
                     /translation="MAIEVSVVRDLTALRALESEWRALAGSAPGALFRGPDWLMPWWL
                     AYHNTLAAELHVMVGRATEADSAGCAVGDLVCFAPFYRRTVKVALLDTRELRMMGDAG
                     PRPPSLDLLVRKGWEDRAGAALAKALVDGAASWDVIDLEPLADPSRIRANLVQRIAPA
                     GFTVESSPSAGGAHRIALALAPSDASDATGVITTHGDDIDSLRKGMSALRRLSRLEWA
                     ERDEPSPLADNEAGSLLEEVTLELGKQGRVRLARLDDAAGEAVAAALVVDDGDRAVVL
                     AMAVDPQVPPKRAARLLHAEALAARARGMVALDVVTGAAEYPMPQLPTSKQQALAVRI
                     WGHSTTASVGRTMTHVAKRARRARETPAVAAAQARAAWTKIRNAASHVAQYDRYGLYR
                     GQLWTRGIEPPQGLEITQLDEAAYDKLDEARRAELMEQLQLDEETARKFWRRGDKSLL
                     ATLGVRPAGIAWYARGPVEVPELGRTLRMGKYDAYIHSVFVAPAARGRAVAPVMLEHL
                     AKELRATDAYRSWALIEAHNQASIRAFQKASFTPVCDVIHAKMATVDRVVCRPPDPEA
                     EELLGLDQ"
     gene            8731..10221
                     /gene="nirK"
                     /locus_tag="VFV99_24000"
     CDS             8731..10221
                     /gene="nirK"
                     /locus_tag="VFV99_24000"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEX5062465.1"
                     /translation="MSRQILLLSVLALAATSCERATPETSKPAAATTNRSQAPEAPDV
                     TSRPTYEVTAELTHAPEVPHRAVRKHPAHVVVNLEVTEVEREIAPGVKYTLWTFGGEV
                     PGKMIRVRQGDVVELHLQNHPNSKMPHNIDLHAVSGQGGGAEGTFTAPGKQTEITFRA
                     QNPGLYVYHCATAPVPMHVANGMYGLILVEPPEGLGWADREFYVMQGELYTTGKYHEP
                     GLQAFDMDKGIDERPTYVVFNGAEGSLVGKNALHAKVGETVRIYVGNGGPNLVSSFHV
                     IGEIFDRVYTEGGSKVQENVQTTVIPAGGSAIVEFKVDVPGTYMLVDHSLFRAFNKGA
                     VGQLIVEGPPMKEIYSGKQPDKPYTGAESTGPIVVASSVGDIGPLDDKGRLELGAATY
                     TRICAACHQAQGQGMPGTFPPLAKSDYLASTAPEKLVGHLVHGLQGALTVNGTQFNGI
                     MPPMAFLTDEEIAGALSFVRSSWGNSGSAIKPETVARVRNQRASAP"
     gene            10221..>10761
                     /locus_tag="VFV99_24005"
     CDS             10221..>10761
                     /locus_tag="VFV99_24005"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5062466.1"
                     /translation="MRLVAIALVVLATAPTSVAAETIAPAKSLAPAKFVAPANTVRVG
                     PGTYRPIFPASPAETDVPVAAFRLDRTPVTNAQFLAFVTAHPEWRRDRAKPLLVDERY
                     LSHWASATNLGDARPAAPVVRVSWFAARAYCASRGGRLPLEREWELAAAASETKRDAS
                     RDPAFQERVLAWYTQLAPAV"
BASE COUNT         1769 a         3636 c         3526 g         1830 t
ORIGIN      
        1 gcctcaccgg aacgccggaa ttccgcatcc cggccggcga cgccgaccac aacgagcaca
       61 tgcgcatcac cgtgccgccg ctgggcgatc tgcagcaagt gcagctctac tcggtcaacc
      121 cgcacatgca catgatcggc acgcacatcg cgacgaaggt cgagcgcccg gcggcgcgcg
      181 gcaacgatcc gcagaacgag tgcctcgcga acgggaaatg gaacttcgac tggcagcgca
      241 cgtacatcta cgacgcaccg ctcgaccagc tgccgagcct caagcccggc gacgtgatcg
      301 atctccagtg caagtgggac aacacgatcg cgaatccgtt cgtgcagcgt gcgctcaagg
      361 acgcgggact cgtcgcgccg atcgacgtca acctcggcga gcaatcgctc gacgagatgt
      421 gcctcgagat catgggcttc tcgatcgacg cgccgccgcc gatgacgcgc acggtgccga
      481 cgatggacga cctgccgatg gcgctgctcg acacgctgaa gcgcaactag agttgcgtcg
      541 cgagcacgac gccgccgccg atcgcgacca cggccacgat gatccagatc agcttgcgga
      601 tcgcagcttc gcgcgcggcc atcttgccca tgtcgaagct cttcgtcgtg cgttgcggga
      661 cctgcgcgcc gaccggcgct agccagtcgt tcggcgcgct ctgctggtac tgcgggtagc
      721 ccggctgctg cgtgccgtac tgctgctgca tgtacggacc cgggaccggc gtcggcggcg
      781 cgtactgctg tgggtacggc gtcggtatcg cgccgggcgc ctgcggatac ggcgtcggca
      841 tcacggccga ctgtcgaggc ggcatcggcg acagcggcgg cggtacagat gaggccggcg
      901 gctgcgcgag cggcggcgga ttcgacggcg gccgcggttg tgagaacgga gcagcgacga
      961 gtgccggcac cggcgtctcg tagcgcccga gcggacgcgg cgttggaatt ccgggggcct
     1021 gtggcacggg cggcggctgc tgcggcgtga gcgtgcgcgg taccgccggt gattgtggcg
     1081 gtggcgccga cgcagagcgc gcgctcgacc gcatgctgac cgtcggtggc gacgctgccg
     1141 gcattggggg cgtcggaaac tcgagaccgg agtcttcgtt gcgcggcggc agcgtgcgcg
     1201 gcagcgtcat gtcgcgatcg ctcttgctct cgcgcggcac tgtcatcgcg cggtcgacct
     1261 catcgcgcgg cagcgtcatc gcgcggtcgc tcatgtcgcg tcccccctgc gcgcgcggcg
     1321 ggatgcgctt gccaacgtcg gcaagtcgcg cgacctcggt cacgcgctcc tcgctgggcc
     1381 ggacgctgac gccatcgtag tcgtcgccgg tctgcgcgag cacgtcggag atctcggaca
     1441 tgtgcgcgat cttcgtgacg ccctcgtgcc ccggtgacga gcgcggcggt ggtgcgccgg
     1501 ccggtcgacg attcgtcgtc ggcgcgtact cgtcttcatc gctccactcg tcgtctgcgt
     1561 tggctcgcgc atcccgcatt tgcgcgagca gcttttcggg cggcggcgtg aactgcgtcg
     1621 cttcttcggc cgatggacgc ttcggccgcc gcgactccgc gatcagtttc tcgggcggcg
     1681 gcgtgaactg cgtcgcttcc tcgaccgacg gacgctttgg tcgccgcgcc tccgcgagca
     1741 gctgtgcaga tggttgtgcg gcggtcgtcg cttccggtgc ccacggatca tcgcgccgcg
     1801 aacgatcgac cagaccgatc ggcggcggcg tgaactgcgt cgcttcgtca tgcttctcga
     1861 cgggtttcgt atcgaccgcg cgtgcgcgat cgggtgcggc gccaccgagc agccgctcca
     1921 gatcgccacg cagctcggcg acggtctgat agcgcgtctc gatgtctcgc tggagacacc
     1981 gcatcacgat gcgatcgagc tcctcgggca ccggcgcgcg cagatacaac ggatccggtt
     2041 tcgtcttcat caccgccgcg agcgcctgcg ctgccgtcat cgcttcaggg aacggcgtgc
     2101 agcccgcgat catctcgtac atcacgatgc cgaccgtgta gatgtcgctt tggccggtga
     2161 ccttggcgcc gaccatttgc tccggcgaca tgtaatcgag cgtcccgatc atctggcccg
     2221 cgttcgtgat gtccgcagca tcgaagtcgc tgccgcgcag gatcttggcg ataccgaagt
     2281 cgagcacctt cacgaagtcg acatcgtcgg ccttcgtctc gagatggatg ttcgtcggtt
     2341 tgagatcgcg gtgcacgatg ccggcagcgt gcgcttcctc gagcgactcg cagacctggt
     2401 gtgcgatccg caccatgcgc ttccactcga acggtccgtt cgccttgtag cgttcgaaca
     2461 agctctcgcc gtggagcagc tccatcacga tgaacaacgt gcggtcggcg gcttgtgcga
     2521 gctcgtacgc ggtgatcgtg tgcggattgc gtagcgcggt cagcgtgtcg ccctcgcggc
     2581 ggaatcgcgc gacgacgccg agatcttgtg cgagcgacgg cagcaacacc ttcagcgcga
     2641 tcggatgacc gctcttgaca tgcgtcgcgc ggtaaatcgc gccgaagcca ccagtcgcga
     2701 tgcgctcttc gatgcgaaac cgtttatcga agatcgtgcc gaccagcgga tctgcccgct
     2761 ccaccgatgt atggacgtcg ctgtccctgc tcggcacgtg cccgatctta cgccgaaagt
     2821 acccgtagca cgcgaatcat cgtgaccagc tctttcccta gcgcggtcag cggcgggcgc
     2881 tgcgagcccg tatgtccata caaaccagtg atgtgcacgc gcacagaccg cgcgtttacg
     2941 aggcgcgatg ccaggacctg cgattgctcg aacgggatca cgtcatcgtc catcccgtgc
     3001 acgagctcga cgcgattcgt gatgcgcgac aggtacggcg ttggatcgag cgccgttgcg
     3061 tcgaactgcg cgagtgctgg caccgcgagg tcccaggcgc ctggctgcgc gccaatgccg
     3121 atcagaaaca gctcgcgcac tgcatcggga atcgatggtg caagctcgtg cgcaaccgcg
     3181 acgaaccgat cgcgcgcttt catctccggc cgtccccacg tgcgttcgac gtagctgcgc
     3241 caccctgcga ccaattcgtc acgcgtgtgc tggtgatgat cgatgtgctc gagcaggttc
     3301 atcagcacga ccggctgatt caacggatcg cgcgtcgctt gccggcccgt cgacacctcg
     3361 ccggtcaggc agaacttcat cgtctcgtgg aagtcggcat agccgccgaa gatgacgagc
     3421 ttgtcgatcg cgtcaccgcg ctcggccgcg agcgcgaacg cgagcagcga gccgaacgag
     3481 atcgagaaca caacgggttt ggtccccgcc ggcagcgcat cgaagacctt tgcgaagtcc
     3541 gagatcgcac gagcatttgg tgtgagggcc aagtagtccg gaacaaacgg cgcaacgacg
     3601 tgatgtccgc tcgccgccaa gatccgacag aagcgatcca tccgcacatc gtccggaccc
     3661 gcgtaatgca acccaggagc gatcaggaat gtcgtcgcgc cgcgcggcgg tcgtaacgcg
     3721 gtcgcaactg gagcgctgtg ccgatggtcg tcgccgacat cgcgcgcggc ttcggccgcc
     3781 ttcgccccgc gtcgccgaaa cacgcgcacg cggatatcgc cgaccttctc gtcgacgata
     3841 tccaccgtcg gggctttcgt cgcatcggcc cagggtccga gccagcgcgc gagtgtgatg
     3901 agccccatcg gcagacgata tctgcaccgc cgcgatcatg cgtggcggcg cgtgcgcgat
     3961 ggctacggga agatgccgcg ctctttgtag cagtgctgca ggcgctcgag accgatcgcg
     4021 tagctcgccg tgcgccagtc cgtcttctgc gtcgccgcgt actggcgaac gttcgcgtag
     4081 gtctgcttca tgcggcgctc gaggcgaccg agcacatccg cgagctccca ctgctccgcg
     4141 cgcttgttct gcagccactc gtagtagctg acgatcacgc cgcccgagtt gcagagaatg
     4201 tcggggatca cgtcgatgcc cttgtcgagc aggatctctt cggcctcggg gtacgtcggg
     4261 ccgttcgcgc cctcgacgac catcttgacc ttgagcgcct tcgcttcgac gacgccgacc
     4321 tcgagctcga gtgctgcggg cacgaagatg tgcgcgtcga gcgagaagaa ctcttcacgc
     4381 gtgatcttcg acgcaccctt gtagcccgac accgagcccg tcttgtggac gtgctcgccg
     4441 agcttgtgcg ggttaatgcc ctcggtgttc gcgatgtagc cgccgacgtc gccgacgccg
     4501 atgatcacgg cgcccttctg cgagagtaga cgcgccgcgt acgaaccgac gttaccgaag
     4561 ccctggatga tgacgtgcgc accgtcgagg ttgaagttct tgtccttcgc ccactccgtg
     4621 atgcagtgaa cgacgccctg gccggtcgct tccgcgcggc cgtgcgagcc gcccgcggtg
     4681 acgctcttgc cggtgacgac gccgcgcacc gtgttgcggt cgctcgcgcc gacgatgttc
     4741 atgtacgtgt ccatcatcca gaccatcgtc tggctgttgg tgttcacgtc gggagccggg
     4801 atatcgaagt ccggaccgat gttgctgccg agggcagcgg tgaaccggcg cgtgatgcgc
     4861 tcgagctcca ccttcgagta ctgacgcggg tcgaacttca cgccgccctt accgccgcca
     4921 aacggaatct cgtgcagcgc gctcttgtac gtcatccacg acgcgagcgc cttcatctca
     4981 tcgaggttcg cctcgtggtg gtagcgcatg ccgcccttgt acgggccgag gatgttgttg
     5041 tgctggactc gatagccctt gagcatctcg atcttgccgt tgtcgcggcg gaccgggaag
     5101 ttgacgatca actcgttctt cggttgcccg aggatctcct tgatgtccga gtcgatgttg
     5161 atcagcttca tcgccttgtc gatctggcga gtagcgacct cgaacaggtt gccgcgatcc
     5221 ttcaggctca cgtcggttcc ggtctgcgtt cgcccgactg cgggcgcgac cggcggtggt
     5281 gcagtgctca tttgccccat ggcgtaccgc ccggaacggg tatgtcgcaa tcaaatctgc
     5341 tgaccatttc gcttatttac gcgccgcgcc atagtaggcg cggatgaacg atcaccagct
     5401 cgcagcgcaa gtgtccgagg gcggccgatt gcgcgcgctc gtgctcgagc tctggcagcg
     5461 gacgcggatg gactggggct tcgtcaccga tcggttagca accacgttca ggaaggaaac
     5521 gtgggtcggc gcacacgagc ggcggttcgt cagtgaaact ctttacggca tggtccgcca
     5581 cctccggcgg atcgatctcg cgctcgagcg cggccgccgc gtgaagaaac caccgcgcga
     5641 tctcgagcgg ctgctcgcgc tgctcgtgct cgacaagctc ctcgaggtcg atcgcgccgc
     5701 gcgcatcgaa cccgatctcg actggcgtgc gatcgcgagc gtcgatgatg tgatcgctgg
     5761 cgagcgtaag ctggcgaagc ggatcgcgct cgcagcatca ctgccggact ggctcgccga
     5821 aacgctcgtc gcggattggg gcgacgacgc cgaaccactc gcgctcgcgc tcaaccaacg
     5881 cgcaccgatg accgtgcgcg cgaatttgct cgtgggcgat cgcgctgcgc ttgcggccga
     5941 gctgacgaag gaaggcctga agacacacgc cggcgcgtgg agcgacaccg cgctgatcat
     6001 cgacagccgc acgaacttgt ttgcatcagc cgcgttcaag cgcggcgcga tggaagcgca
     6061 agacgaaggc agccagctgc tcgccgatct cgcagtaccg accgcgacga agtcgctcgt
     6121 gatcgattac tgcgccggtg ccggtggcaa gacgctcgcg atcgcagcgc ggctcgccaa
     6181 ccgcggccgg atcgtcgcgt ccgatgtcga cggcaagaag ctcgaagagc tgcgccggcg
     6241 cgcgcgccga ggcggcgtat cgaacgcgca ggcgatcgcg atcgaaggcg gcaagtggcc
     6301 gcgcgagctc gacgcgctcc gcgcgaaagc tgacgtcgtg ttcgtcgatg cgccgtgctc
     6361 cggcatcggt gcgttgcgcc gcaatcccga agcgcgctgg cgaatgcgcc agaccgacat
     6421 cgcagcgttc gcggcgcgcc agcgcgagat catggcagcc gcactcgacc tcgctgcgcc
     6481 tggcgcgcgt gtcgtctatg caacttgctc gctcttgcgc gccgagaacg aagacgtcgc
     6541 gcagtcgatc gcgggcactg gtacctgcga gctcctgccg ctgtccgacg tgctcggcga
     6601 tcgcgccaac gtgttcggcg gagaggcgtt caccgttgcg ccacatacgc atggcactga
     6661 cgggttttac gcacgaatca tgcgccggct gtaaccgggc gaatccacga ccgcgattga
     6721 agccgctttt ggtaactcgg ctgagcgaaa agctccgcgt ggccaacgcc gccggaacgg
     6781 gccatgcatt cccaacagta tggaccccgc ggaacgcgat accgcgacga catcgtcaac
     6841 acccgacgtt gccaacagca acgcgcaggc tcttgcacgc cgtgatacgt tcgaacgccg
     6901 gatggcgatc gaagtcagcg tggtgcgcga tttgacagcg cttcgcgctc tcgaatccga
     6961 gtggcgcgct ctcgccggct ctgcgcccgg cgcgttgttc cgcggccccg attggctgat
     7021 gccgtggtgg ctcgcctatc acaacaccct cgccgccgag ctccacgtga tggtcggccg
     7081 cgcgacggaa gccgactctg ctggttgcgc agtcggtgac ctcgtctgct tcgcgccgtt
     7141 ctatcgccgc accgtcaagg tcgcactact cgacacgcgg gagctgcgaa tgatgggcga
     7201 cgccgggcca cgtccgccgt cactcgatct gctcgtgcgc aaaggctggg aagatcgcgc
     7261 cggcgccgca ctcgcgaagg cgctcgtcga cggagccgca tcgtgggacg tgatcgatct
     7321 cgagccgctc gcggatccgt cgcgcattcg cgcaaacctc gtacagcgca tcgcgcctgc
     7381 tggcttcacc gtggagagct cgccgagtgc aggtggcgcg catcgcatcg cactcgcgct
     7441 tgcaccatcc gacgcgagcg acgcaaccgg cgtgatcacc acgcatggtg acgacatcga
     7501 ctcgcttcgc aaaggcatga gcgcgttgcg ccgcctgtcg cgtctcgaat gggccgagcg
     7561 cgacgagccc tcgccgcttg ccgataacga agctggatcg ctgctcgaag aagtcacgct
     7621 cgagctcggc aagcaaggcc gcgttcgact cgcgcgtctc gacgatgcag cgggtgaagc
     7681 cgttgccgcc gcgctcgtcg tcgacgatgg tgatcgcgcg gtcgtcctcg cgatggccgt
     7741 cgatccgcag gtgccgccga agcgcgccgc tcgtctgctc cacgccgaag cgctggcagc
     7801 tcgtgcgcgc ggcatggtcg cgctcgacgt cgtcaccggc gctgccgagt atccgatgcc
     7861 ccagttgccg acgagcaagc agcaagcgct cgccgttcgc atctggggac actcgacgac
     7921 cgcatcggtc ggtcgcacga tgacgcacgt cgcgaagcgc gcacgtcgcg cccgcgaaac
     7981 gccggcggtt gccgccgcgc aggcacgggc cgcgtggacg aagatccgca acgccgcatc
     8041 gcacgtggcg caatatgatc gatacggcct ctatcgcggc cagctgtgga cgcgcggcat
     8101 cgagccgcca cagggcctcg agatcaccca gctcgacgaa gccgcctacg acaagctcga
     8161 cgaagcgcgc cgcgccgagc tcatggaaca gctgcagctc gacgaggaga ccgcgcgcaa
     8221 gttctggcgc cgcggcgaca agtcgttgct tgcaacgctc ggcgtgcgcc ctgccggcat
     8281 cgcgtggtac gcgcgtggcc ccgtcgaagt gccagagctc ggccgcacgc tgcgcatggg
     8341 caagtacgac gcatacatcc acagcgtgtt cgtcgcgccg gcagcacgcg gtcgcgcagt
     8401 cgcgccggtc atgctcgaac atctcgccaa agagctgcgc gccaccgacg cctatcgctc
     8461 gtgggcgctg atcgaagccc acaatcaggc ttcgattcgc gcgttccaga aagcgtcgtt
     8521 cacaccggtg tgcgatgtga tccacgcgaa gatggcgacg gtcgatcggg tcgtgtgtcg
     8581 gccgccggat cccgaagccg aagagctgct cggcctcgat cagtagcgcg caacaattgc
     8641 ggctcggaag gagccgtatg cagtacgtgc gagcatcggc tagcaattct ggtggtttag
     8701 gcgggcaccg tggttgctct tacggacagc gtgtcccggc aaattcttct cctatcagtc
     8761 ctcgcgctcg ccgcgaccag ctgcgaacgc gccacccccg aaacctccaa acccgccgct
     8821 gccacgacga accgttcgca agcgccggaa gctcccgacg tcacgtcgcg gccgacgtac
     8881 gaggtcacgg ccgagctgac gcacgcgccc gaggttccgc atcgcgcggt tcgcaaacat
     8941 ccggcgcacg tcgtcgtcaa cctcgaggtc accgaggtcg agcgcgaaat cgcgcccggc
     9001 gtgaagtaca cgctgtggac gttcggcggc gaggtaccgg gcaagatgat ccgcgtgcgg
     9061 caaggcgacg tcgtcgagct gcacttgcag aaccacccga acagcaagat gccgcacaac
     9121 atcgacctgc acgcggtgtc cggtcagggt ggtggcgccg agggcacgtt caccgcgccg
     9181 ggcaagcaaa ccgagatcac gttccgtgcg cagaacccgg gcctctacgt ctatcactgc
     9241 gcgactgcgc cggtgccgat gcacgtcgcg aacggcatgt acggcctcat cctcgtcgag
     9301 ccgcccgagg gtctcggctg ggcagaccgc gagttctacg tgatgcaggg cgagctctac
     9361 acgacgggca agtatcacga gcccggcctg caagcgttcg acatggacaa gggcatcgac
     9421 gagcgcccga cgtacgtcgt gttcaacggc gccgaaggct cactcgtcgg caagaacgcg
     9481 ctgcacgcga aggtcggcga gaccgtgcgc atctacgtcg gtaacggcgg gcccaatctc
     9541 gtgtcgagct tccacgtgat cggcgagatc ttcgatcgcg tctacaccga aggcggcagc
     9601 aaggtgcagg agaacgtgca gaccacggtg attcccgctg gtggctccgc gatcgtcgag
     9661 ttcaaggtcg acgtgcctgg cacgtacatg ctcgtcgatc actcgctgtt ccgcgcgttc
     9721 aacaaaggtg cggtcggtca gctgatcgtc gaaggcccgc cgatgaaaga gatctacagc
     9781 ggcaagcagc ccgacaagcc gtacaccggc gccgagtcca ccggtccgat cgtcgtggcc
     9841 agctccgtcg gcgatatcgg tccgctcgac gacaaaggcc ggctcgagct cggcgccgcg
     9901 acgtacacgc ggatctgcgc cgcgtgccac caagcacaag gccagggcat gcccggcacg
     9961 ttcccgccgc tcgcgaagtc cgactacctc gcgtctaccg cgccggagaa gctcgtcggt
    10021 cacctcgtgc atggtctgca gggagcgctc accgtcaatg gcacgcaatt caacggcatc
    10081 atgccgccga tggcgttcct caccgacgag gagatcgcag gcgcgctcag cttcgtgcgg
    10141 tcctcctggg gcaatagcgg cagcgcgatc aagcccgaga ccgtggcgcg ggttcgtaac
    10201 caacgtgcga gcgcgccgtg atgcggctcg tcgccatcgc gctcgtcgta ctcgccaccg
    10261 caccgacctc cgtggcggcg gaaaccatcg cgcccgcgaa atcgcttgcg ccggcgaaat
    10321 ttgtcgcgcc tgcgaacacg gtacgcgtcg gccccggaac ctaccggccg atctttcccg
    10381 cctcgcccgc cgagacggac gttccggtcg ctgcgttccg tctcgatcgt acgccggtga
    10441 cgaatgcgca gttcctcgcg ttcgtgaccg cgcatcccga gtggcgccgc gatcgcgcga
    10501 agccgctgct cgtcgacgag cgctatctct cgcattgggc gagcgcgacc aacctcggcg
    10561 atgcgcggcc ggccgcgccg gtcgtgcgcg tcagctggtt tgccgcgcgt gcgtattgcg
    10621 cgagccgcgg tggccggctg ccactcgagc gcgaatggga gctcgccgcg gcggcgagtg
    10681 agacgaagcg cgatgcgtcg cgcgaccccg cgtttcagga gcgcgtgctc gcctggtaca
    10741 cgcaactcgc gccggcagtt c
//
LOCUS       DASUFX010000598         6481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_375309,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000598 DASUFX010000000
VERSION     DASUFX010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375309"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2411
                     /locus_tag="VFV99_24010"
     CDS             <1..2411
                     /locus_tag="VFV99_24010"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5062467.1"
                     /translation="HIREAWDYTHDDAPFEEQEVLVTVPASFDEAARELTLQAATAAG
                     YPPVVLLEEPQAAFYAWIDNARTKLGPGERVLVFDVGGGTTDFTLIEVDANGDGFTRT
                     AVGDHLLLGGDNLDLTLAKIVEQRVVARTNKKLDALQWHGLVHACRLAKETLLGDDPP
                     ATAPIVVQARGAKLIGGTIRDEVSRDELDKILFDGFFPLVGKDAQLARGRSGLQEFGL
                     PYAADPAVTRHLAVFLQRHHAERVDAILFNGGAMTPTSLRQRVLDQIGQWQGSAPREL
                     PAAMPEMAVAQGAAYYGLVRRGLATRIKGGTARAFYIGVDASGMRRRVPTTEATAAGA
                     AVEPARYAVCLAPPGLDDGARVELERDFRLVTNRPVSFRLYSSSTREDHPGALVPIGD
                     GKAETIDDGSDLLELPPIVTVLRARGRGEVTVRLAVHITALGALEIYCVDTEAPNETW
                     KLAFDMRSGGAAPSVEAVAESAPHPKTDEAKAKIKAAFTTSAGLQTLTRDLETLLEAR
                     RDEWSMMTTRALFDALVEVAADRKKTADHEQRWLNLAGFLLRPGTGAPLDAWRSRVMW
                     GVFNENLAHPKSEPGKLAWWIVWRRIAGGLAKGQQDQIYDRLAQLLLPSTKQAKKLAE
                     AKPSKQELAEMWRAVASMERLPIEHKVKLGDELIHRMDSRKGREDPVHLWALSRIGAR
                     VPLYGPLNALVPPSKVRAWLEPLVTWDWPEPDKAAFPLAQLGRRTGDRTRDLDDATRT
                     KLAELLRTLPGGERAAVLVEQVVALEAREERVALGDTLPAGLKLVVDDERAEDLSREL
                     DSPS"
     gene            complement(2408..3919)
                     /locus_tag="VFV99_24015"
     CDS             complement(2408..3919)
                     /locus_tag="VFV99_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062468.1"
                     /translation="MEGLMIRLRHACIAVLFAACSPVSDSSNVPDAAVDAPNTPLMIV
                     SSAPNNNAKSASILNPVSVFVNKKLDPATVNATTVKLRYGYAEGWYGLYGTVSYDEAS
                     LKISFVPGQPLYTGVFFEVVLDGVKSADGSETLSNQRIRFSSIVNYQKRQTSFQATGK
                     VSSWYEYMLDMNGRQTASMGHQSAGLDGIWFTMDDPKGCCKYTQAYDAEGRNTEYRYY
                     GNMGPDGQPWTADDVLTSFQKYNYDATSGQMTTIDVFGGGPDGVLGTADDVITAYQSY
                     SYDSNGYVTGYFYFTSAGPDGAWHTADDRGQPNQQWGDWIVDSYGNVTKYIYRTFGAD
                     GLPKTADDVINSVSEQMYDQYGYQTRSIYYNTAGADGMWNTADDVVGGYSKTDFDADH
                     LPIRYWNYNATSVPPGIGPDNMWFTADDVPSYLSTNSFNELKLSTEQMGYQDKGPDGV
                     FNTADDVASYRSTSEYESNGARVNYTGYNPGPDGKYKTADDTISYKSEYDLEH"
     gene            complement(4022..6094)
                     /locus_tag="VFV99_24020"
     CDS             complement(4022..6094)
                     /locus_tag="VFV99_24020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5062469.1"
                     /translation="MADLRWSWLAAPYVVTTALILAVSVTAALIRGDRVMRLGVVAAS
                     SSAVPWSMCQGLAALTDDPETARHLIKLGQGPVVAVGPMLMLVMLGVSGQLERYRWIL
                     RIGAIICGLSFIVCWGTSWVVPGVQKLPSGMFYMAPGPLTGFYLSQLLAWLGVGLVLQ
                     RRATPRGERKRTFRLLMIVLVVATIGSMDTLLLYRIWGVYPIAWLSACVAAVLALYLV
                     LKTDILRPQGFDRSVALEMGLFLASTVLTVGLTILLGTSSPLPVVTLSAIGWALMTGL
                     AWASARRRRTRVKGERELERFMARVPTFEDEKKISEKLGALWQNAVSVDLTTLWWRDG
                     ASLTNHEGTRWRLDRAVNAWLVQNNEPLIVVDLATMRVGMIRPDLESLAAAHDADVIV
                     PLVDRGELIGLVEAKCPHVLREADRGLIADSGKAAARALTFVGLARAANRERETARQV
                     EVADALRLQASASRETELGRWAVAAEYRTAPRSTGAGWQAIELGDGRLALLVTEAQAH
                     GVAAALATAALTGAFAAATASGGPVTLDEILRTMRASSEGVLRGGEPVAAFLAILDGP
                     NSTIDWACAGHPGAMLIGPLAAVEAGLPAGSLSGPRPKAKAFTGGPRVRGASLHAARR
                     GQDQYFSDYVLVVASTALRGDDDVKWEQRLLEVAPASGRIASVLVTSALEAGEPAEDL
                     LAVVVRSR"
     gene            complement(6105..>6481)
                     /locus_tag="VFV99_24025"
     CDS             complement(6105..>6481)
                     /locus_tag="VFV99_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062470.1"
                     /translation="PVSDCVAVPVAINDCMLASDAADWVSFVPSSVCTAVKVEARLTF
                     PIAHEELSITLWDIDHDMQVGTDTECAASGDEANSRRCLDVTLVPGTKYGIEVKPTGE
                     GNCNGACAYNRYSLSVGLATPG"
BASE COUNT         1127 a         2236 c         2097 g         1021 t
ORIGIN      
        1 gtcacatccg cgaagcgtgg gactacacgc acgacgacgc gccattcgaa gagcaagaag
       61 ttctcgtaac cgtacctgct tcattcgacg aagccgcacg tgagctgacg ctgcaagccg
      121 caactgccgc cggctatccg ccggtcgtgc tgctcgaaga gccacaggcc gcgttctacg
      181 catggatcga caacgcacgc acgaaactcg ggcccggcga acgcgtgctc gtgttcgacg
      241 tcggcggtgg caccaccgac ttcacgctca tcgaagtcga cgcgaacggc gacggcttca
      301 cgcgcaccgc cgtcggcgac cacttgctgc tcggcggcga caacctcgat ctcacgctcg
      361 cgaagatcgt cgagcaacgc gtcgtcgcgc gcacgaacaa gaagctcgat gcgctgcagt
      421 ggcacggcct cgtgcacgcg tgccggctcg caaaggagac gctgctcggc gacgacccgc
      481 cggcgaccgc accgatcgtc gtgcaggcgc gcggcgcgaa gctgatcggc ggcacgatcc
      541 gcgacgaggt ctcgcgcgac gagctcgaca agatcctgtt cgatgggttc ttcccgctcg
      601 tcggcaagga cgcgcagctc gcgcgcggcc gcagcggcct tcaggaattc ggcctgccct
      661 acgccgccga tcccgccgtc acgcgtcacc tcgcggtgtt cttgcagcgt catcacgccg
      721 aacgcgtcga cgcgatcctg ttcaacggcg gtgcgatgac accgacgtcg ctgcgccagc
      781 gcgtgctcga tcagatcggc cagtggcagg gctccgcgcc gcgcgagctg cccgccgcga
      841 tgccggagat ggccgtcgcg caaggcgccg cgtattacgg cctcgtccgc cgcggcctcg
      901 cgacgcggat caaaggtggc acagcgcgcg cgttctacat cggcgtcgat gcgagcggca
      961 tgcgccgacg cgttcccact accgaagcca ccgcagccgg cgcggccgtc gagcccgcac
     1021 gctacgcggt gtgcctcgcg ccacctggcc tcgacgacgg cgcgcgtgtc gagctcgagc
     1081 gcgacttcag gctcgtcacg aatcggccgg tgagctttcg gctctactcg tcgagcacgc
     1141 gcgaggatca ccccggtgcg ctcgtgccga tcggcgacgg caaggccgag acgatcgacg
     1201 acggcagcga cctgctcgag ctaccgccga tcgtgaccgt gctgcgcgca cgcggtcgcg
     1261 gcgaggtcac cgtgcggctc gccgtgcaca tcacggcgct cggcgcgctc gagatctact
     1321 gcgtcgacac cgaagcacca aacgagacct ggaagctcgc gttcgacatg cgctcgggtg
     1381 gcgccgcacc gagcgtcgaa gccgtcgccg agtccgcgcc acacccgaag accgacgaag
     1441 cgaaggcgaa gatcaaagcc gcgttcacga cgagcgccgg gctccaaacc ctcacccgcg
     1501 atctcgagac gctgctcgaa gcgcgccgcg atgaatggtc gatgatgacg acgcgcgcgc
     1561 tgttcgacgc gctcgtcgag gtcgccgccg accgcaagaa gaccgccgat cacgagcagc
     1621 gctggctcaa cctcgccggc ttcttgctcc gccccggcac cggcgcgccg ctcgatgcgt
     1681 ggcgctcgcg cgtgatgtgg ggtgtgttca acgagaacct cgcgcatccc aagagcgagc
     1741 ccggcaagct cgcgtggtgg atcgtgtggc ggcgcatcgc cggcggcctc gcgaagggcc
     1801 agcaagatca gatctacgat cgactcgcgc agctgctgct gccgagcacg aagcaggcga
     1861 agaagctcgc cgaggccaag ccgagcaagc aggagctcgc cgagatgtgg cgcgcggtcg
     1921 cgagcatgga gcgcttgccg atcgagcaca aagtgaagct cggcgacgag ctcatccacc
     1981 gcatggactc gcgcaagggt cgcgaagatc cggtgcacct gtgggcgctg tcgcgcatcg
     2041 gcgcccgcgt gccgctctac ggcccgctca acgcgctcgt gccgccgagc aaagtgcgag
     2101 cgtggctcga gccgctcgtg acatgggatt ggcccgagcc cgacaaggcc gcgttcccgc
     2161 tcgcgcagct cggccgtcgt accggcgatc gcacacgcga tctcgacgac gcgacacgca
     2221 ccaagctcgc cgagctgcta cgcacgctgc ccggtggcga gcgcgcggct gtgctcgtcg
     2281 agcaagtcgt cgcgctcgaa gcgcgcgagg aacgcgtcgc gctcggcgac acgttgcccg
     2341 ccggcctcaa gcttgtcgtc gatgacgaac gcgccgagga tctgagccgc gagctcgaca
     2401 gcccgagcta atgctcgagg tcgtattcgg acttgtagct gatcgtgtcg tcggcggtct
     2461 tgtacttgcc gtcgggaccg gggttgtaac ccgtgtagtt cacccgggcg ccgttcgatt
     2521 cgtactccga ggtgctgcgg tagctggcga catcgtccgc ggtgttgaac acgccgtcgg
     2581 gtcccttgtc ctggtatccc atctgctccg tggagagctt cagctcgttg aacgagttgg
     2641 tgctgaggta gctcggcacg tcgtcggccg tgaaccacat gttgtccgga ccgatgccag
     2701 gtggcacgct ggtggcgttg tagttccagt agcggatcgg cagatggtcg gcgtcgaagt
     2761 cggtcttcga gtagccaccg accacgtcgt cggcggtgtt ccacatgccg tcggcaccgg
     2821 ccgtgttgta gtagatggag cgcgtctggt agccgtactg gtcgtacatc tgctccgaga
     2881 ccgagttgat cacgtcgtcg gcggtcttgg gcaagccgtc ggcgccgaag gtccggtaga
     2941 tgtacttcgt cacgttgccg taggaatcga cgatccaatc accccactgc tgattcggct
     3001 gaccgcggtc gtcggcggtg tgccacgcgc catcggggcc ggcactggtg aagtaaaaat
     3061 agccggtcac gtagccgttc gaatcgtagc tgtagctctg gtacgcggtg atcacgtcgt
     3121 cggcggtgcc gagcacgccg tcagggccgc cgccaaagac gtcaatcgtc gtcatctgac
     3181 ccgacgtcgc gtcgtagttg tacttctgga acgacgtcag aacgtcgtcc gccgtccagg
     3241 gctgtccgtc gggccccata ttgccgtagt agcgatactc ggtgttccga ccttcggcat
     3301 cgtaggcctg cgtgtacttg caacacccct tcgggtcgtc catcgtgaac caaatgccgt
     3361 cgagccccgc gctctggtgg cccatgctcg ccgtttgccg cccgttcatg tcgagcatgt
     3421 actcgtacca gctcgacacc ttgccggtcg cttggaacga ggtctggcgc ttctgatagt
     3481 tgacgatcga cgagaacctg atccgttggt tcgaaagcgt ctcggaaccg tcggcgctct
     3541 tcacaccatc gaggacgacc tcgaagaaca caccggtgta gagcggctga cccggcacga
     3601 acgaaatctt cagcgaggcc tcgtcgtacg acacggtgcc gtagagcccg taccagccct
     3661 cggcgtagcc gtagcgcagc ttcaccgtcg tcgcgttcac cgtcgccgga tcgagcttct
     3721 tgttcacgaa gaccgagacc ggattcaaga tgctcgcgct cttcgcgttg ttgttcggcg
     3781 cggacgacac gatcatgagc ggcgtgttcg gcgcgtcgac tgcagcgtcc ggcacgttgc
     3841 tcgaatcgct gacggggcta caagcagcaa acagcaccgc gatgcacgca tggcgcaggc
     3901 gaatcattag accctccaca gagatttcca tcctaatacg gattgcgagg gcttcgcgac
     3961 cccgtgggtg tgacgcgcag catggaacgc agcgtcctcc ctgaatcgcg cttggggtag
     4021 ctcagcgaga tcggaccacg acggcaagca gatcttcggc cggctcaccg gcttcgagcg
     4081 cgctggtgac gagaacggac gcgatccggc cactcgcagg tgcgacttcg agtagccgtt
     4141 gctcccactt cacgtcatcg tcaccgcgaa gtgcggtaga cgcgacgacc agcacatagt
     4201 ccgagaagta ttggtcctgg ccgcgtcgcg ctgcgtgaag gctcgctcca cgcacacgtg
     4261 gaccacctgt gaatgccttc gccttcggcc gcggtccgct cagcgagccg gccggcaagc
     4321 cggcctcgac ggccgcgagc gggccgatca gcatcgcgcc cggatgaccg gcgcacgccc
     4381 agtcgatcgt cgaattcggg ccgtcgagga tcgcgaggaa cgccgcgacc ggctcgccgc
     4441 cgcgcagcac accttccgag ctcgcgcgca tggtgcgcag gatttcatcg agcgtcaccg
     4501 gcccaccgct cgcggtcgcc gccgcaaacg cgccggtcag tgccgccgtg gcgagcgccg
     4561 ccgctacgcc gtgtgcctgg gcttcggtca cgagcagcgc gagccggcca tcaccgagct
     4621 cgatcgcctg ccagcctgca ccggtcgagc gcggcgccgt gcggtactcc gccgcaacgg
     4681 cccagcggcc gagctcggtc tcgcgactcg ccgatgcctg caagcgcaac gcgtccgcga
     4741 cttcgacctg gcgcgcggtt tcgcgctcgc gattcgccgc acgcgcgagc ccgacgaacg
     4801 tcagtgcacg cgccgccgct ttcccggagt cggcgatgag cccgcgatcg gcttcgcgca
     4861 gcacgtgagg gcatttcgcc tcgacgagtc cgatgagctc gccgcgatcg acgagcggca
     4921 cgatcacgtc ggcgtcgtgc gcggccgcga gcgactcgag gtccgggcga atcatgccga
     4981 cgcgcatcgt cgcgagatcg acgacgatga gcggctcgtt gttctgcacc agccacgcgt
     5041 tgaccgcgcg atcgagccgc cagcgcgtac cttcgtgatt cgtcagagac gcgccatcgc
     5101 gccaccacag cgtcgtgagg tcgacgctga ccgcgttctg ccacagcgcg ccgagcttct
     5161 cgctgatctt cttctcgtcc tcgaacgtcg gcacgcgcgc catgaagcgc tcgagctcgc
     5221 gctcgccttt gacgcgcgtg cgtcgtcgcc gcgccgacgc ccaggcaagg ccggtcatca
     5281 gcgcccaacc gatcgccgac agcgtgacga ccggcagcgg gctcgacgtg ccgagcagga
     5341 tcgtgagccc gacggtcagc accgtcgacg cgaggaacaa tcccatctcg agcgcgacgc
     5401 tgcgatcgaa gccttgcggg cgcaggatgt cggtcttgag cacgagataa agcgcgagca
     5461 ccgccgccac gcacgccgac agccacgcga tcggatacac gccccagatc cgatacaaga
     5521 gcagcgtgtc catcgagccg atcgtcgcga cgacgagcac gatcatcagc aagcgaaacg
     5581 ttcgcttgcg ttcaccgcgc ggtgtcgcgc gccgctggag cacgaggccg acgccgagcc
     5641 acgcgagcag ctgcgatagg tagaagccgg tcagcggacc cggcgccatg tagaacatgc
     5701 ccgacggcag cttctgcacg ccgggaacga cccacgacgt tccccagcag acgatgaacg
     5761 acaggccgca gatgatcgcg ccgatgcgca agatccagcg ataccgctcg agctggccgc
     5821 tgacgccgag catgacgagc atcagcatcg gaccaaccgc gaccacaggc ccttggccga
     5881 gcttgatcag gtgccgcgcg gtctcgggat cgtcggtcaa tgcagcgagc ccctgacaca
     5941 tcgaccacgg caccgccgac gacgacgcgg cgaccacacc aagacgcatc acgcgatcgc
     6001 cacggatcaa cgcggcggtc acagacaccg cgaggatcaa cgcggtggtt acgacgtatg
     6061 gcgcagcaag ccagctccac cgaaggtcgg ccactgcata caggttaacc cggcgtggcc
     6121 agcccgacgc tcaacgaata gcgattgtat gcgcacgcac cgttgcaatt gccttcgccg
     6181 gtcggcttca cctcgatgcc gtatttcgtg cccggcacga gcgtgacatc cagacaccgg
     6241 cgagagttcg cctcgtcgcc gctggccgcg cactccgtgt cggtgccgac ctgcatgtcg
     6301 tggtcgatgt cccagagcgt gatcgagagc tcctcgtgag cgatcgggaa cgtgagtcgc
     6361 gcctcgacct tgacggcggt gcacaccgac gacggtacga aggacaccca gtccgcggca
     6421 tcggacgcga gcatgcagtc gttgatggcg accggcaccg ccacgcaatc cgacaccggg
     6481 a
//
LOCUS       DASUFX010000599        11756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1485894, whole genome shotgun sequence.
ACCESSION   DASUFX010000599 DASUFX010000000
VERSION     DASUFX010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485894"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2203
                     /locus_tag="VFV99_24030"
     CDS             <1..2203
                     /locus_tag="VFV99_24030"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5062471.1"
                     /translation="MAAHKYWMPPDQVFDTEAQWTASPFATALNFIKSCGQNSSNPAC
                     IWKEIPTKLAGDDAGGALRNPVSLPDDELAKQATTLLGMNKNVSGVTTCSECHAANQT
                     TLRDWQMKTDTAMASCLKNTTDGGAPTTEKKEDVSIDKDEFKTWGPFEVALGGKIEVK
                     MAGTGDADLYVKRNAEPTTESYDCRPFSREPNEQCLSTQFNANGPAKFYVGAAGFADH
                     SKITITVTYGKPDANTPKPKDIVDCLRLEPGRSDSPYVPEKAGIYAAAAHLGFFQAVF
                     KAAFPDGEGSNNADTWALEYGKFKNRVSMPKGNHPRLTQGELDIVAEWFARGLPRLTT
                     YIAPDTGPTTCMQNISSNVQSHVSAMSTQGWQAINRGANLRMYGCGTSSNPRECLTSA
                     ANANSKSYGMGWAATGTLRVLRELSFNTYYWMRSSADGRFVANGSTGGGGAVISDLQS
                     DKDMSAHAAYDPGFFPDNRGWMFQGTPIGSAFCTTGLLVRNPDRIEFNEPECSHVEGV
                     SLYQHLGAALGGGDYFAVNNVFSSDNPSGVVTKDMSAGFGPTATMRFTAMMFDGTRYV
                     GKPTVSQSSPHEGDTVLSPSTKLVISRFGNENAQLGYVLRRLDATPTGSSYTVSTPEI
                     ARYCVQGNKASISYDEKYFVTHHYVGPNDWQDLGFSSAQDPTFQQMLAKGTSNIILVN
                     MVTGARTRITNMKPGQYALFPHWRSDGWIYFQVRDKNTSKEYIVASDAALP"
     gene            2213..3904
                     /locus_tag="VFV99_24035"
     CDS             2213..3904
                     /locus_tag="VFV99_24035"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5062472.1"
                     /translation="MRAVVVTLLVVAACGDDAVDKTAMCKLGTAKSAATVQDRKIIGA
                     VAPYQADLGLAARDEELRTSIAARRQVAWDAVAKVLAPTPLAEPMLAASFGGQPSIPA
                     WHTWFAREDFDRVFKKVYRDLGPAARRARADLDAATIDAGFDWNTHAVEQDPSWPEQR
                     YLDYLAVIDTQEEAQGIAGVNRVGYSSGAMRHLIHSYEQQYSCRVNPPPDPYAPEGVR
                     EGGMVKQVDTLAVDECEWRVLGPFVASAGAKVKVTSTGDGDADIYVRKGAAPEAHDFD
                     CRSSGDASTESCTVAGGGLVYVAVFGAQASNVEVDVEYLAEDVALPTCLDGEMPRDAV
                     LVKADWRRKLTGENLPVFDTSPARMRERFALKADWGSGDGAANPDPSSIYTITLPNGN
                     EFRMPALHVMTKELDHWLWITLWWSNNPDTDFGADRPASVASLPGPWRNYKMCVTSAY
                     LEGDPDPRGGAGGSLGDAIESVHGGVGAPSWCSNPYLEMGEGNAATNCIGCHQHGGTD
                     LTPEAILATLPHSGSTRVRNNFFTDYLWAIKGGQGEDLSSLVQAEVDYWDANDPP"
     gene            4120..8544
                     /locus_tag="VFV99_24040"
     CDS             4120..8544
                     /locus_tag="VFV99_24040"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEX5062473.1"
                     /translation="MTGDLELSVLQRPSGGLGYIAGFAITDQLIVAAGGTSSRHPTVV
                     VSSNARHFEMRKTPRMLGLRDVLAVGDALWTCGEYGQLAMSRDGGVTWKVFDTGTDAC
                     LFALALVPDGAVWVVGDNGYAARVPPGPGEKLERIDFKTSTRLASVYALRDEVIVLGF
                     DGVMYRWREGDVARIATGATKPLTALAVSKLGTWILVGDGGFVARSPDGAWWSRVKSG
                     VEIDLEAICACPDGRLVVVGDRGLILVSADDGRQWKTVETTLLAHLWSVERFGGGVLI
                     GGDDGLIAKLAPPGDETWSDRINVFGGVKPLDVVFAEGPDGFVVRGLVTYLDAIENAR
                     DDDEDDDDDDEDDTDSEAFSTLNEAGTAQSFEANYGVALSREAAAFVTAIDGRDKWSS
                     FEELRLDNDLLPDVGDKNLFELLVRRDQHAYLGTGLVESFCGVFCIGSQGNGDTYHME
                     LYEWDGPRQVLHFDHETHAFTGVFADSLDSLVYLAALKRANDARGISTEAFEIGLRKL
                     RGKVAPTWHFAIDDDDPEFVALEPKRRDTEFFFYRSRWITALLKNDGVTDIDDIPRLF
                     NADFNQVVPQDQLPARYEACEKFIPTALYSMWRAFLFDEPELVPYLEIGRRHKSRLVR
                     DAATLIDELRDGRNELGTIKDVRAHLAKFRALDLDPRRADQRTAEAEARAKADATRHQ
                     DIVVELERAPRTGWSDLAWRWLDDGVAHRALLAKLDDTPELAAQIAALDELRGVPDTD
                     REVMLPRLADDLAPELEAVLVGSLVRDDALDGVLAAPPKPDDGDGGEDGSDDDEGSPG
                     WDAIDTALAPIYGRGEPHAHFGTVLPYMLGGNDPIHGISVYLRDEPVPHFHFVTYGFT
                     DLFEKETDDPEVSGFGFELTVRLRRAAGETDVPMWTLNFLQNLARYVFGTGNRFGAGH
                     KMGLNGPIALDHDTKLTAILFAEDPELEEISSTFGKAQFLQIVGITDDEYKLIQEWST
                     SGLVEILREQLPLLVTDLARDSVLADRQTAAKVKQRVEQEGSSEDLTFAGEMKLEVSE
                     GRVRIEMGALYAAALPRAMRGRLRHGRGYTLRGRSNMLTLEPGETTGFVQSDGDMTLR
                     LTPDLVIEIEARLRTTLAGRYRFEMWPALEIVVTPSVIRDQAGQAIDVRGVADPAEVK
                     KLIDDENARLAAEVAEHRDDSDDDDADDADDDADDADDADDDADDGAAPPADRVRAAL
                     AMTERALRLAPDDDDAQFTHAMLLLDAERAGIAGSSDKLLRALPNYTISNRVNIAVRM
                     GKAKHPRFGDAVDALLGAKLPHRILAGGGSTNVATYGDVAEELFSELGKLVLASAPDR
                     LVRLAPMLPGRVGMLSALAWQACQADQRDGALALYEQLLLIEIPEAGDDRTNYLRAMN
                     NACVQAHAAKAYDTAVRIADRAQAVAHENPYIYHSAACAYSAVGDYAKAFEQVKLAMK
                     YGYDHLGKVEVDSDLGPLLEWPEFKALFRDWHDRQEGN"
     gene            8544..11636
                     /locus_tag="VFV99_24045"
     CDS             8544..11636
                     /locus_tag="VFV99_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062474.1"
                     /translation="MAVEVHGLDAMEGETAFAFFVVRLREQVSRAQLVELLRYVAPLI
                     PEPETFIEDLPEDLEQSIRILAMPWTPPEAGHAVEAQRRLIESTGSHGVEAAWFFQTG
                     TGLPAVDDVDDDGDDDDGDDDLPPSADSDDPRLRARTDPQRSARAMTTDDSWVGESSW
                     SRETNWSSGEVGGDTKTQPIEIIDVSKVDDAEDEVMTRRAHSALDTVQDDNDDEDELE
                     RAHTDEDDDDDDDDDGDFDDEDDDEDDDDEDDEDDDDDEDDDDDDDSIEAGDDDDVSW
                     SNGPPPQVAKVRFPVDGYPAILDELDWEDFGIAVKLRGDMLAGEGTVLFGFHTLWLAP
                     YGGRFRNAAVTIDRTHHAAHLWVDRFAAPCSAAVQVHHLLWVLSKLDEVVPIVHARFA
                     GATMAQKYAGLMGDTSEPFVLGGNPLVAVHAALGEVGVDHWIAEQKDWSPAEIARMLR
                     ELAIDVVTGHDPVADDGEEDEDDAAGEEVDGVFAASEAEREDEEGDDADDEDDDEEGE
                     LVDDEDDESDGDDDDDDDDDDDDDDEDDDDDDDGEAEGNEDEDRGRQITSYAGELLAA
                     RAAAGKLDPRAAEQLLPVLATSEKFEHRRRAVVQIFGALRYRPAVPAMIRILEETTIA
                     SSLDSIGKEDFVAATAAALGAIGDPSAIDALARVVAAPGTHNDEPRPVAADALASCLA
                     AAPEPRHVDDAVLAELLTTIRERNDGELNAETQLAYGRIVHQLPADKRAEARRRLAES
                     DSARDDAIAVLARQAALLLASPTSPVNAPPRDLQPLLHESLTSVDYDHDYTVRNLRVA
                     LRVAEIVPNLVNADDIVWLTRFAESDIRTRAHSLLAKLHKPLPRAPVFDARAARLLED
                     DALVRMIGDAHVVGRAALIAEAGRRDLVRAKRAIIDACHDVISRARQGGENLLDPDTR
                     VLEAAVPILRDGLLDDDVIALFDRMLRHSNYHVKWELLQKPPHDERLIAGMFHVMAEK
                     WGWQEKTAKQWLANFQGSPAYEAERKRAPVAEDDDERDDEEDDDDDGDDDDEDDEDMN
                     "
BASE COUNT         2152 a         3721 c         4053 g         1830 t
ORIGIN      
        1 catggcggcg cacaagtact ggatgccgcc ggatcaggtg ttcgacaccg aggcgcagtg
       61 gacggcgtcg ccgttcgcga ccgcgctcaa cttcatcaag agttgcggcc agaactcgtc
      121 gaacccggcg tgcatctgga aggaaattcc gacgaagctc gcgggtgatg acgccggcgg
      181 cgcgctgcgc aacccggtct cgctgcccga cgacgagctc gcgaagcagg cgaccaccct
      241 gctcggcatg aacaagaacg tcagcggcgt cacgacgtgc tcggagtgcc acgcggcaaa
      301 ccagacgacg ctgcgcgact ggcagatgaa gaccgatacg gcgatggcgt cgtgcctcaa
      361 gaacacgacc gatggcggcg cgccgacgac cgagaagaag gaagacgtct cgatcgacaa
      421 ggacgagttc aagacttggg gcccgttcga agtcgcgctc ggcggaaaga tcgaagtgaa
      481 gatggccggc accggcgacg ctgacctcta cgtcaagcgc aacgccgagc cgacgacgga
      541 gtcgtacgac tgccgcccgt tctcgcgcga gcccaacgag cagtgcctca gcacgcagtt
      601 caacgcgaat ggcccggcga agttctacgt cggtgcggcg ggcttcgccg accactcgaa
      661 gatcacgatc acggtgacct acggcaagcc cgatgcgaac acgccgaagc cgaaggacat
      721 cgtcgactgc ctgcgcctcg agccgggccg cagcgattcg ccgtacgtgc cggagaaggc
      781 cggtatctac gcagcggccg cgcacctcgg cttcttccag gccgtgttca aggccgcgtt
      841 ccccgacggt gaaggcagca acaacgccga cacgtgggcg ctcgagtacg gcaagttcaa
      901 gaaccgcgtc tcgatgccga agggcaacca tccgcggctc acgcagggcg agctcgacat
      961 cgtcgccgag tggttcgcgc gcggtctgcc gcggctgacg acgtacatcg cgccggatac
     1021 cggcccgacg acgtgcatgc agaacatctc gagcaacgtg cagtcgcacg tcagcgcgat
     1081 gtccacgcaa ggctggcagg cgatcaaccg cggcgcgaac ctgcgcatgt acggttgcgg
     1141 tacgtcgtcg aatccgcgcg agtgtctgac gagcgcggcg aacgcgaaca gcaagtcgta
     1201 cggtatgggc tgggcggcga ccggaacgct gcgcgtgctg cgcgagctgt cgttcaacac
     1261 ctactactgg atgcgcagct cggccgacgg tcgcttcgtc gcgaacggct cgaccggtgg
     1321 tggcggcgct gtgatcagcg acctccagtc cgacaaggac atgagcgcgc acgctgcgta
     1381 cgacccgggc ttcttcccgg ataaccgcgg ctggatgttc cagggcacgc cgatcggctc
     1441 ggcgttctgc acgacgggcc tgctcgttcg caacccggat cgcatcgagt tcaacgagcc
     1501 cgagtgcagc cacgtcgaag gcgtctcgct gtaccagcac ctcggtgcgg ctctcggcgg
     1561 cggcgactac ttcgcagtca acaacgtgtt ctcgagcgac aacccgtcgg gcgtcgtgac
     1621 gaaggacatg agcgcgggct tcggcccgac cgcgacgatg cgcttcaccg cgatgatgtt
     1681 cgacggtacg cgctatgtcg gcaagccgac cgtgtcgcag tcgagcccgc acgaaggtga
     1741 caccgtgctg tcgccttcga cgaagctcgt gatcagccgc ttcggcaacg agaacgcgca
     1801 gctcggctac gtgctccgcc gcctcgatgc gacgccgacg ggctcgtcct acacggtgtc
     1861 gacgccggag atcgcgcgct actgcgtgca gggcaacaag gcctcgatct cgtacgacga
     1921 gaagtacttc gtgacgcacc actacgtcgg cccgaacgac tggcaggacc tcgggttctc
     1981 gtcagcgcag gacccgacgt tccagcagat gctcgcgaag ggcacgtcga acatcatcct
     2041 cgtcaacatg gtgaccggcg cccggacccg catcacgaac atgaaaccgg gccaatacgc
     2101 gctgttcccg cactggcgct cggatggttg gatctacttc caggttcgtg acaagaacac
     2161 gagcaaggaa tacatcgtcg ccagcgacgc cgctcttccg tagaatcggc tcgtgcgcgc
     2221 cgtcgtagtc acactgcttg tcgttgctgc ttgcggtgac gacgcagtcg acaagacggc
     2281 gatgtgcaag ctcggcaccg cgaagagcgc ggcgaccgtg caggatcgca agatcatcgg
     2341 cgcggtcgct ccgtatcaag cggacctcgg gctcgctgcg cgcgacgagg aattgcggac
     2401 gtcgatcgcg gcgcgccgtc aggtcgcgtg ggatgcggtc gcgaaagtgc tcgcaccgac
     2461 gccgctcgcg gagccgatgc ttgcagcgag cttcggcggg cagccctcaa tcccggcgtg
     2521 gcacacgtgg ttcgcgcgcg aggacttcga tcgcgtgttc aagaaggtgt atcgcgacct
     2581 cgggccggcc gcgcgccggg cacgcgctga cctcgatgcc gcgacgatcg atgcgggctt
     2641 tgactggaac acgcacgcgg tcgagcagga cccgagctgg cccgagcagc gctacctcga
     2701 ttacctcgcg gtgatcgaca cgcaggaaga agcgcagggc atcgctggcg tcaatcgcgt
     2761 tggctactcg agcggtgcga tgcggcacct catccacagc tacgagcagc agtacagctg
     2821 tcgcgtgaat ccgccgccgg atccgtacgc gccagaaggc gtgcgcgagg gcggcatggt
     2881 gaagcaggtc gatacactcg ccgtggacga gtgcgagtgg cgcgtgctcg ggccgttcgt
     2941 cgcgagcgcg ggtgcgaagg tgaaagtgac atcgaccggt gacggcgatg cggatatcta
     3001 cgtgcgcaag ggcgcggcgc cggaagccca tgacttcgat tgccgctcgt cgggtgatgc
     3061 gagcaccgag tcatgcacgg tcgctggcgg cgggctcgtc tatgtcgcgg tgttcggcgc
     3121 acaggcgtcg aacgtcgagg ttgacgtcga gtatctcgcc gaggatgtcg cactgccgac
     3181 gtgcctcgat ggtgagatgc cgcgcgacgc ggtgctcgtc aaagcggatt ggcggcgcaa
     3241 gctcaccggc gagaacctgc cggtgttcga tacgtcgccc gcgcgcatgc gcgaacggtt
     3301 cgcgcttaag gcggactggg gaagcggtga cggcgcggcg aatcccgatc cgtcgtcgat
     3361 ctacacgatc acgttgccga acgggaacga gttccggatg cccgcgctcc acgtgatgac
     3421 gaaggagctc gatcactggc tgtggatcac gctgtggtgg tcgaacaatc ccgataccga
     3481 tttcggcgcg gatcgtcccg cgagcgtggc gtcgctgccc ggcccgtggc gcaactacaa
     3541 gatgtgcgtc acgagcgcgt atctcgaagg cgatccagat ccgcgcggcg gtgcaggtgg
     3601 ctcgcttggc gacgcgatcg aatcggtgca cggcggcgtc ggcgcgccga gctggtgctc
     3661 taacccgtac ctcgagatgg gcgaaggcaa cgcagcaacg aactgcatcg gttgtcacca
     3721 gcacggcggc actgatctga cgcccgaagc gatcctcgcg acgctgccac acagcggctc
     3781 gacccgcgtc cgcaacaact tcttcacgga ctatctgtgg gcgatcaagg gcgggcaggg
     3841 cgaggatctg tcgtcgctcg tgcaggccga ggtcgattac tgggacgcca acgatccgcc
     3901 gtagcggggg ccggaacgcg aaggagctcg ccgggagagg aggagacgac gagctcgatc
     3961 gcgtcctgaa accgagcatc tgttaaaacg gataagttgt caacagagcg gattgcggca
     4021 cccccgtcga atcgagcaaa gaccgcgcga tggctcggaa tttcggccac aaatccggag
     4081 gcgcagcgtg cgtcggctcg ggcagaatcg gcggcgagga tgacagggga tctggagctc
     4141 tcggtgctgc agcgcccgag cggtgggctc ggctatatcg ccgggtttgc gatcacggat
     4201 cagctcatcg tcgcagcggg cgggacgtcg agtcggcatc cgacggtcgt cgtgtcgtcg
     4261 aacgcgcggc acttcgagat gcgcaagacg ccgcgcatgc tcggcctgcg cgacgtgctc
     4321 gcggttggcg atgcgctgtg gacgtgcggt gaatacggcc agctcgcgat gtcgcgcgat
     4381 ggcggcgtga cgtggaaggt attcgatacc ggaaccgacg cgtgtctgtt cgcgctcgcc
     4441 ctcgtgcccg atggtgcggt gtgggtggtc ggcgacaacg gctacgcagc acgcgtgccg
     4501 ccgggtcccg gcgagaagct cgagcgcatc gacttcaaga cctcgacgcg gctcgcctcg
     4561 gtatacgcgc tgcgcgacga ggtcatcgtg ctcggcttcg acggcgtgat gtatcgctgg
     4621 cgcgaaggcg acgtcgcgcg gatcgcgacg ggcgcgacga aaccgctgac cgcgctcgcg
     4681 gtgagcaagc tcggcacgtg gatcctcgtc ggtgatggtg gcttcgtcgc gcgctcgccg
     4741 gacggcgcgt ggtggtcgcg cgtgaagagc ggcgtcgaga tcgatctcga ggcgatctgc
     4801 gcgtgcccgg atggccgttt ggtcgtcgtc ggtgaccgtg ggctcatcct cgtgtcggcc
     4861 gacgatggcc ggcaatggaa gaccgtcgag accacgctgc tcgcgcacct gtggtcggtc
     4921 gagcggttcg gcggtggtgt gttgatcggt ggcgatgacg gcctgatcgc aaagcttgct
     4981 ccgccgggcg atgagacgtg gagcgatcgc atcaacgtct ttggcggcgt gaagcccctc
     5041 gatgtcgtgt ttgccgaagg cccggacggc tttgtcgtgc gtggcctcgt gacgtacctc
     5101 gatgcgatcg agaatgcgcg cgatgacgac gaagacgatg acgacgacga cgaggacgac
     5161 actgacagcg aggcgttctc gacgctgaac gaggccggca ccgcgcagag cttcgaggca
     5221 aactacggag tcgcgctgtc gcgcgaggcg gcggcgttcg ttaccgcgat cgatggccgt
     5281 gacaaatggt cgagcttcga ggagcttcgc ctcgataacg atctacttcc cgacgtcggc
     5341 gacaagaatt tgttcgagct cttggtgcgg cgcgatcagc acgcgtatct cggcaccggg
     5401 ctcgtcgaat cgttctgcgg cgtcttctgc atcggctcgc agggcaacgg cgacacctat
     5461 cacatggagc tctacgagtg ggacggccca cgtcaggtgc tccatttcga tcacgagacg
     5521 cacgcgttca ccggcgtgtt cgccgactcg ctcgacagcc tcgtctacct cgccgcgctc
     5581 aagcgtgcga acgacgcgcg agggatctcg accgaagcgt tcgagatcgg gttgcgcaag
     5641 ctgcgcggca aggtcgcgcc gacgtggcac ttcgcgatcg acgacgacga tcccgagttc
     5701 gtcgcgctcg aaccgaagcg acgcgacacc gagttctttt tctatcgctc gcgctggatc
     5761 actgcgctgt tgaagaacga cggcgtcacc gacatcgatg acatcccgcg gctgttcaac
     5821 gccgatttca atcaggtcgt gccgcaggat cagctgcctg cgcgctacga ggcgtgcgag
     5881 aagttcattc cgaccgcgct gtactcgatg tggcgcgcgt tcctgttcga cgagcccgag
     5941 ctggtgccgt acctcgagat cggccggcgc cacaagtcgc ggctcgtccg cgatgctgcg
     6001 accctgatcg acgagctccg cgacggccgc aacgagctcg gtacgatcaa ggacgtgcgt
     6061 gcgcatctcg caaagttccg cgcgctcgat ctcgatccgc gccgtgccga tcagcgcacg
     6121 gcggaagccg aggctcgcgc gaaggcggac gcgacgcgcc atcaggacat cgttgtcgag
     6181 ctcgaacgcg caccgcgcac gggctggagc gacctcgcgt ggcgctggct cgacgatggc
     6241 gtcgcgcatc gtgcgctgct cgccaagctc gatgacacgc cggagcttgc agcgcagatc
     6301 gcggcgctcg acgagttgcg cggcgtgccg gataccgatc gcgaagtgat gctgccgcgg
     6361 ctcgccgacg atctggcgcc cgagctcgag gcggtgctcg tcggcagtct cgtgcgcgac
     6421 gacgcactcg acggcgtgct cgcggcaccg cccaagccgg acgatggtga cggcggcgag
     6481 gacggcagcg acgacgacga gggttcgcct ggctgggacg cgatcgacac ggcgctcgcg
     6541 ccgatctacg gccgcggcga gccgcatgcg catttcggga ccgtgctgcc gtacatgctc
     6601 ggcggcaacg atccgattca cggcatctcg gtctatctgc gcgacgagcc ggtgccgcac
     6661 tttcacttcg tcacatacgg cttcaccgat ctgttcgaga aggagaccga cgatccggag
     6721 gtcagcgggt ttggtttcga gctgacggtt cggttgcggc gcgccgccgg cgagaccgac
     6781 gtgccgatgt ggacgctcaa cttcctgcag aacctcgcgc gctacgtgtt cggcaccggc
     6841 aatcgcttcg gtgccggtca caagatgggg ctcaacggtc cgatcgcgct cgatcacgac
     6901 acgaagctga ccgcgatcct gttcgccgaa gaccccgagc tcgaggaaat ctcgtcgacg
     6961 ttcggcaagg cgcagttcct tcagatcgtc ggcatcaccg atgacgagta caagctgatc
     7021 caggagtggt cgacgagcgg tctcgtcgag atcttgcgcg agcagttgcc gctgctcgtg
     7081 accgacctcg cgcgtgactc ggtgcttgcc gataggcaga cggcagcgaa ggtgaagcaa
     7141 cgcgtcgagc aggaaggctc gtcggaagac ctgacgtttg ccggcgagat gaagctcgag
     7201 gtctccgaag gtcgcgttcg catcgagatg ggcgcgctgt acgcagcggc gctcccacgc
     7261 gcgatgcgcg gccggctccg gcacggtcgc ggctacacgt tgcgcggccg gagcaacatg
     7321 ctcacgctcg agccgggcga gaccactggg ttcgtgcaga gcgacggcga catgacactg
     7381 aggctgacgc cggacctcgt gatcgagatc gaggcccggt tacgcacgac gctcgcaggt
     7441 cgctaccggt tcgaaatgtg gccggcactc gagatcgtgg tgacgccgag cgtgatccgc
     7501 gatcaggccg gtcaggcgat cgacgtgcgt ggcgtcgccg accctgccga ggtgaagaag
     7561 ctgatcgacg acgagaacgc gcgcctcgcc gcggaagtag cggagcaccg cgatgactct
     7621 gacgacgatg acgccgacga cgcagatgac gatgccgacg atgccgacga cgccgacgac
     7681 gatgccgacg atggtgcggc gccgccggcg gaccgcgtgc gtgcggcgct ggcgatgacc
     7741 gagcgcgcgc ttcgtctcgc acctgatgac gatgacgcgc agttcaccca cgcgatgctg
     7801 ctgctcgacg ccgagcgtgc ggggatcgcc ggcagcagcg acaagctcct gcgcgcgctg
     7861 ccgaactaca cgatcagcaa tcgcgtcaac atcgcggtgc gaatgggcaa ggcgaagcat
     7921 ccgcggttcg gtgatgccgt cgatgcgttg ctcggcgcga agctcccaca tcggattctg
     7981 gcaggcggcg gcagtacgaa cgtcgcgacc tacggcgacg tcgccgagga gctgttcagc
     8041 gagcttggca agctggtgct cgcgtcggct cccgaccgac tcgtcaggct cgcgccgatg
     8101 ctgcccggcc gcgtcggcat gctgagcgcg cttgcctggc aggcgtgcca ggccgaccaa
     8161 cgcgacggcg cgctcgccct gtacgaacag ctactgctca tcgagattcc cgaggcgggc
     8221 gacgaccgca cgaactacct gcgggcgatg aacaacgcat gcgtgcaggc gcatgctgcg
     8281 aaggcgtacg acacggcggt gcggatcgcc gaccgcgcac aagcggtcgc gcacgagaat
     8341 ccgtacatct atcactcggc ggcgtgcgcg tattcggccg tcggtgacta cgcgaaggcg
     8401 ttcgagcagg tgaagctggc gatgaagtac ggatacgacc acctcggcaa ggtcgaggtc
     8461 gactccgacc tcggaccgct actcgagtgg cccgaattca aggcgctgtt ccgcgactgg
     8521 cacgatcgtc aggagggcaa ctgatggccg tcgaggtaca tggcctcgat gcgatggagg
     8581 gcgagactgc cttcgcgttc tttgtcgtgc gcctgcgcga gcaagtctct cgcgcgcaac
     8641 tcgtcgagct gctgcggtac gtcgcgccac tgatcccgga gcccgagacg ttcatcgaag
     8701 acctgccgga agatctcgag cagtcgattc gcatcctggc aatgccatgg acgccgcctg
     8761 aagccggcca tgcggtagaa gcgcagcggc gattgatcga atcgaccggc tcgcacggcg
     8821 tcgaggcagc gtggttcttc cagaccggca cggggctgcc ggccgtcgat gacgtcgatg
     8881 acgacggcga tgacgatgac ggcgacgacg acctaccacc gagcgccgat agcgacgatc
     8941 cgcgtttgcg agcacgcact gatccgcagc gctcggcgcg tgcgatgacg acggatgata
     9001 gctgggtcgg cgagtcgagc tggagccgcg agaccaactg gagcagtggc gaggtcggcg
     9061 gtgacaccaa gacgcagccg atcgagatca tcgacgtctc gaaggtcgac gatgctgaag
     9121 acgaggtgat gacgcggcgc gcgcactctg cgctcgatac ggtacaggat gacaacgacg
     9181 acgaggacga gctcgagcgg gcgcataccg acgaagacga cgacgatgat gatgacgacg
     9241 acggcgactt cgacgatgag gatgacgacg aagacgacga cgacgaggat gacgaagacg
     9301 acgacgacga cgaggatgac gatgacgacg acgacagcat cgaggccgga gacgacgacg
     9361 atgtgagctg gtcgaatggg ccgcccccgc aggtcgcgaa ggtccgcttt ccggtcgatg
     9421 gctaccctgc aatcctcgac gagctggact gggaggactt cgggatcgcg gtcaagctgc
     9481 gcggcgacat gctcgcgggc gaaggcaccg tgctgttcgg gtttcacacg ctgtggctcg
     9541 cgccgtacgg cggacgattt cgtaacgcag cggtcacgat cgatcgcacg caccacgcag
     9601 cgcacctgtg ggtcgatcgg ttcgcggcgc cgtgcagtgc ggccgtccag gtccaccatc
     9661 tcttgtgggt gctgtcgaag ctcgacgagg tcgtcccgat cgttcacgcg cgcttcgccg
     9721 gtgcgaccat ggcgcagaag tacgccggcc tcatgggcga tacgagcgag cccttcgtgc
     9781 tcggaggcaa tccgctggtc gccgtccacg ccgcgttagg cgaggtcggc gtcgatcact
     9841 ggatcgccga gcagaaggac tggtcgccgg ccgagatcgc gcggatgctg cgcgagctcg
     9901 cgatcgacgt cgtgaccgga cacgatcccg tcgcggacga cggcgaagaa gacgaggacg
     9961 acgccgcggg cgaggaagtc gacggcgtgt tcgctgccag cgaagctgaa cgcgaagacg
    10021 aggaaggcga cgacgccgac gacgaggacg acgacgagga gggcgagctc gtcgatgacg
    10081 aggacgacga gagcgacggc gacgacgacg acgatgatga tgacgacgac gacgacgatg
    10141 acgaggatga tgacgacgac gacgatggcg aggccgaggg caacgaggat gaagaccgcg
    10201 gccgccagat cacgagctac gcgggcgagc tgcttgcggc acgtgccgct gcgggcaagc
    10261 tcgatccgcg cgcggccgag cagctgctgc cggtgctagc gacgtcggag aagttcgaac
    10321 accgccggcg tgcggtcgtc cagattttcg gcgcattgcg ctaccggccg gccgtccccg
    10381 cgatgatcag gattctcgaa gaaacgacga tcgcgagctc gctcgattcg atcggcaagg
    10441 aagactttgt cgcggccact gcggcggcgc tcggcgcgat cggcgatccg tcggcgatcg
    10501 atgcgctcgc acgcgtagtc gctgcaccag gcacgcacaa cgacgagccg cgtccggtcg
    10561 cggctgatgc cctcgcgtcg tgtctcgccg ctgcccccga gccgcggcac gtcgacgacg
    10621 ccgtcctcgc cgagctgctc acgaccatcc gcgagcgcaa cgacggcgag ctcaatgccg
    10681 agactcagct cgcgtacggc cgcatcgtgc atcagctgcc tgccgacaag cgggcagaag
    10741 cacgccgtcg gctcgccgaa tcggacagcg cccgcgacga cgcgatcgcg gtgctcgcgc
    10801 gccaagcggc actactgctc gccagcccga catcgccggt caatgcgccg ccgcgcgatc
    10861 tgcagccgct gctgcacgag agcctgacct cggtcgacta cgatcacgac tacacggtgc
    10921 gaaacctgcg cgtcgcgctg cgagtcgccg agatcgtacc gaacctcgtc aacgccgacg
    10981 acatcgtctg gttgactaga tttgccgagt ccgacatccg cacgcgcgcg cactcgctgc
    11041 tcgcgaaact tcacaagccg ttgccgcgag caccggtctt cgacgcgcgc gccgcgcgac
    11101 tactcgaaga cgacgcgctc gttcgcatga tcggcgacgc gcatgtcgtt ggccgcgctg
    11161 cgctgatcgc cgaggcaggc cgtcgcgacc tcgttcgcgc gaagcgggcg atcatcgatg
    11221 cgtgtcatga cgtgatctcg cgtgcgcgtc agggcggcga gaacctgctc gaccccgaca
    11281 cccgcgtgct cgaggctgca gtaccgatcc tgcgcgacgg gctgttggac gatgacgtga
    11341 tcgcgttgtt cgatcggatg ctgcgccact cgaactacca cgtgaagtgg gagctgttgc
    11401 agaagccgcc gcacgacgaa cgtttgatcg cgggcatgtt ccacgtcatg gcagagaagt
    11461 ggggctggca agagaagacc gcgaagcagt ggctcgctaa cttccagggg tcgcccgcct
    11521 acgaggccga gcgcaagcgc gcgccggtcg cggaggacga tgacgagaga gacgacgaag
    11581 aagacgacga tgacgacggt gacgacgatg acgaagacga cgaggatatg aactgaccgc
    11641 ggtgctggag tcggatcgcg attacaccgt cgagctcgat ctcgacgagc ccgacgacgc
    11701 ccaggcgatc gcgcgcaagc tcgccgagca agtcggcgtt ccgccgaatg agctgc
//
LOCUS       DASUFX010000600        10457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_515113,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000600 DASUFX010000000
VERSION     DASUFX010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515113"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..770
                     /locus_tag="VFV99_24050"
     CDS             405..770
                     /locus_tag="VFV99_24050"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5062475.1"
                     /translation="MPEHDELFPHPTPELAEQRKRLAPGPAESFRAFGKSVFSEGAIS
                     AKTKQLIAVAVAHVTQCPYCIRGHTRAALRHGATPEELMEAIWVAAEMRAGGAFAHSA
                     IALDEIARHEHESNTTPRP"
     gene            complement(779..1228)
                     /locus_tag="VFV99_24055"
     CDS             complement(779..1228)
                     /locus_tag="VFV99_24055"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEX5062476.1"
                     /translation="MAVAALATGEPLVFPSLGPTAFMLFYQPRSAAASPRNALIGHLI
                     GIVSGYVSLRLFGLQDAMPALTGGVTLAWVGSSSLSLALTSGLMIWLRVPHPPAGATT
                     LIVSLGILPRPVQLVALFAGIVALVLQGLVINRLAGVRYPLWAGPKD"
     gene            complement(1392..3806)
                     /locus_tag="VFV99_24060"
     CDS             complement(1392..3806)
                     /locus_tag="VFV99_24060"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5062477.1"
                     /translation="MKGWELVYEGYEPMQEGVRETLCTLGNGYFATRGAAHDAAGGVH
                     YAGTYITGCYNRLSTEIDGRLLEHEDLVNMPNWLLLTYRHAGGEPFELATTEILSYRQ
                     TLDLRRGIVTRDVRVRDRKGCTTRITSTWIVHMADPHVAALEVVLTAEDWSGPIEIRA
                     ALDGWVQNRGVEIYRLLASQHLAPIDSRAVGEDSVLLEMETVRSHIRIAEAARTRVFD
                     GDRPYAHGSAAITRPGFAARELSLELPKNRPIRIEKVVAIYTSRDFAIAAPDIAATER
                     IAAVPTFATLVESHVLAWERLWRAFDICVEVAGTSEPSTGVILRLHVFHLLQTASEHS
                     ADLDAGVGARGLHGEAYRGHVFWDELFIFPLLNTRMADITRALLMYRYRRLAAARRAA
                     RREGYRGAMYPWQSGSDGREETPLQYFNPRSGRWIVDNTHLQRHVGAAIAYNVWQYFQ
                     VTGDVDFLGDIGAEMFLEIARFVASLATYVPERGRFEIRGVVGPDEFHTAYPGAHEPG
                     LDNNAYTNVMSVWMLWRARDVLDALPRDRRHDVCKKLALGDDELVRWDEISRKMWLPF
                     LDGGILAQFEGYDRLLELDWADYRARYGNIQRLDLILESEGDSPNRYKASKQADVLML
                     YYLFSSEELRTLFERLGYPFDPSAIPRTIDYYLRRTSDGSTLSRVVHAWVLARSRRAG
                     SWQIARDALASDIRDIQNGTTREGIHLGAMAGSVDLIQRGYTGLELREGVIWINPRLP
                     DPVERLDVLVRYRGHTLELTIWGDVLEVKAIEAAIPTIKLGVLGTVIELRAGERRRFA
                     LREAQS"
     gene            complement(3803..4564)
                     /locus_tag="VFV99_24065"
     CDS             complement(3803..4564)
                     /locus_tag="VFV99_24065"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5062478.1"
                     /translation="MRIDLAIYRAFALDLDGVVTNTAAVHAAAWKQLFDELLERRAAG
                     RTWLPFDSDREYRAYVDGKPRRDGLRGFLAARGISIPEGTPGDSADAETIHALAKRKN
                     KYVHERIARDGVQVYGDAVALLRRARASGVRTAVVTASENGGPVLAAARITGLFDARV
                     DGLEIARLGLRGKPASDSFVEAARRLGVPVARTAVFEDAIAGVQAARAGRFGLVIGID
                     RNGHGEALRESGADHVVSSLDEIELVSDATRMELR"
     gene            complement(4692..5087)
                     /locus_tag="VFV99_24070"
     CDS             complement(4692..5087)
                     /locus_tag="VFV99_24070"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX5062479.1"
                     /translation="MKRAYEPVEASDGYRVLVERLWPRGLRKADAHIAEWLKELAPSH
                     ELRKWFGHEPTRFSEFRQRYLRELRAAPARLALAKLASRAARGNVTLIYSAHDEVHNN
                     AVVLARELERRLARNRVKPTRHARPVHAT"
     gene            complement(5212..6060)
                     /locus_tag="VFV99_24075"
     CDS             complement(5212..6060)
                     /locus_tag="VFV99_24075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5062480.1"
                     /translation="MSDLAGKTFVITGANTGIGKITARELARRGAHVILACRSLDKTQ
                     PVIDEIKKETGNDKIEFVALDLADLASVRRCAEELLARKIPIHGLINNAGLAGSRGTT
                     KDGFELTFGTNHLGHYLFTRLLLDRIKESGPARIVNVASASHYQARGINWDALRAKTK
                     SRTGLPEYAVSKLSNVLFTKELARRLEGSQVTTYALHPGVVATDVWRRVPGPVRWVMK
                     KFMVTPEEGAQTSIRCATAPELASETGRYYDRDGKERRPNKLANDEELAKQLWTKSAE
                     WTGLPA"
     gene            6237..6704
                     /locus_tag="VFV99_24080"
     CDS             6237..6704
                     /locus_tag="VFV99_24080"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX5062481.1"
                     /translation="MKRLVVVLALFGVVLAFGLACGGKPHRASTAAHPSGPVLYDRIG
                     RMDAIKGIVKDFVEEQLKKGTLAGRFSNVDTAQLEDNLARQLCELTGGPCKYTGRTMH
                     EAHASLAITSADFTAFVAAFEQSLVKFKVEPQEQNELLALYRKQHDDIVTQPQ"
     gene            complement(6748..7713)
                     /locus_tag="VFV99_24085"
     CDS             complement(6748..7713)
                     /locus_tag="VFV99_24085"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5062482.1"
                     /translation="MSSRVRIANTIGVVLAVGLALVAASYRAPMGSLAAAPALTPCLE
                     IIEMPGGRRGLRDATGQVVELKAYRRIVAGSLVADRLLRELAEPDRVVGVSGYGRAQS
                     PWAYQQADKPVLASTDDVEAVLSLAPDLLLLNSFGESAKVARLRERGIAVFDLGAMGG
                     VDMLVRDIHIVAQLLGHAERGERLATAWQRRFAAVASALHGRARKRALLLTTYGGKVF
                     GGTRGTSYGDVLGAAGLVDVAATSYTGWPQYTAEELLRLDPDLIVTKYGMGAQLCRIG
                     GLDQLKACRSSSGFVEIDGALLDDPGLPMLDAAEAIFAIAYPDAK"
     gene            complement(7710..8468)
                     /locus_tag="VFV99_24090"
     CDS             complement(7710..8468)
                     /locus_tag="VFV99_24090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5062483.1"
                     /translation="MGDALIVDNVTVRFASRVAVANASFTAAHGAVTAILGPNGAGKT
                     TLLRAIAGLAAYEGTIWVGGVDVNRLPLRARARHIGYVPQQSRLDSPLSAFEVVAQGR
                     YTYAQGVGRSSATDHDAIEDALRRADALDFADRSFVELSHGQRKRVLIARALATGARL
                     VLFDEPTASLDIGHVLAFFRLARELAAAGTAVVVVLHDLDEARRHCDGAVLLQAGSVA
                     ARGLVADVITRDPVRKVYGIELVPGDALGFREVT"
     gene            complement(8461..9453)
                     /locus_tag="VFV99_24095"
     CDS             complement(8461..9453)
                     /locus_tag="VFV99_24095"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5062484.1"
                     /translation="MKSYRAWLAVIVTASVLIVIGSLFVGRGDLGNRALVSTFLELRG
                     MRAAAAFLSGAALAVAGVVLQTLFRNPLVDPAILGTTAGASLGGQLAILGFSFAPAWL
                     RPAFLVPEMVLPLGCLIGALFALTLLLAVIQHTRSELAVLLTGFLLSSLFVSVGALLI
                     SLVQESWELGRAVISFTLGGVGGAGLRHVLIGLPLVVAAFIALWAWSRPLELMLSGQD
                     EATTLGVDVRQVRWWTVLWVAVGTSAAVAIGGNVGFVGLVVPHVLRPLVGISPRRLLP
                     AAALAGGAFLLLCDVVIRAAPFHGELPLGVITGLVGGPLFLVLVVRSQREIAHG"
     gene            complement(9472..>10457)
                     /locus_tag="VFV99_24100"
     CDS             complement(9472..>10457)
                     /locus_tag="VFV99_24100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062485.1"
                     /translation="SGDVGLPSQPQPMNLLVTIDHANSALTWIDPTTCTPLRQLDVST
                     GFYANPHDVIAVSSMKAYVARYEKNTTPTADPADRDEGDDLLIIDPSVPAITGRIDLA
                     SYAGSGVQARPDRARLIDGKLFVLLSNLSGDFSTAAEGRLVVVDTATDQVSGTIDLPG
                     LKNCGGLSYVESTKTLVVACGGSFSDPEQAASSGIAYVDTAASPPAMTRKQDMTPFDG
                     RALAGFSGIAREGATGFGVTFGNFGGSPTDQFWALNVQAGTTRKVADASDSFTFGTVL
                     VDPSHERVYLTDAKTAMPRVHVYTYATAITREASINANPAIGLPPREIAWY"
BASE COUNT         1839 a         3490 c         3424 g         1704 t
ORIGIN      
        1 gtgacacgat cggtgcctcg tccacgtcgc ttgcgcgaca gctcgacgcg acgggagcgt
       61 ttgacatctg tcgtaccggt ggtagctgct cggtatggag acgaacgcgc gaaggagctg
      121 ggatgtcgtc gaccaagcaa gtctcgagtc gtttcctgcg agcgatccac ccggatgggg
      181 atcgtatcgc gccgcaccat cggcagcgtc gatcgcggaa acaacggtcg tggccgagcc
      241 ggttcggtca gccaccgcgc gcgcgtctgt gaccaaatgg ttcgtcgctg gtgcgagcct
      301 gctcgtatcg gcgatcttcg gtttgcgatt cgttcgtcgg tacgtccagc ggcatcgagc
      361 acaccacgat tgacggcacg caatcctcta cgagcgagga acccatgcca gagcatgacg
      421 agctgtttcc ccatccaaca cccgagttgg ccgagcaacg caaacgactt gcgcctggac
      481 ctgccgagtc gtttcgagcc ttcggcaaga gcgtcttttc cgaaggggcg atttcggcca
      541 agacaaagca gttgatcgcc gtggcggtcg cacacgtgac gcagtgcccg tattgcatcc
      601 gaggccatac gcgggcggcg ctgcgtcacg gcgccacgcc ggaggagctc atggaggcga
      661 tctgggtagc cgcagagatg cgcgcgggcg gtgcatttgc gcattcggcg atcgcactgg
      721 atgaaattgc tcgtcacgag cacgaatcga acacgacgcc gcgcccgtga tggtgcactc
      781 agtctttcgg gccggcccag agcggataac gtacgcccgc gaggcggttg atcacgaggc
      841 cctgaaggac gagcgcgacg attcccgcga acagcgcaac gagctgcacc ggacgcggca
      901 agatcccgag actcacgatc agagtcgtgg cgcccgctgg cggatgcggc acgcgcaacc
      961 agatcatgag gcccgaggtg agcgcgagcg acaacgatga cgaacccacc cacgccaatg
     1021 tcacgccgcc cgttaatgca ggcatcgcat cctgcagccc gaacaatcgc agcgagacat
     1081 atccagagac gatcccgatg agatggccga tcagcgcgtt gcgcggcgag gccgccgccg
     1141 acctcggctg ataaaacagc atgaacgccg tcggcccgag cgatgggaac acgagcggct
     1201 caccggtcgc caacgcggcg acagccatta gcgcagtcga caacccacca ttcacgagag
     1261 cgaacacgcc cgtccacagc gtcgagtcag ttcgttgggc gcgtgaccgt gcgcgagccc
     1321 gcacgagcaa tccgtgcacg acctccccga gctcgccatg gtgtcgtcga ggtgcggcca
     1381 tccggcgtgt ctcagctctg tgcctcgcga agcgcgaacc tgcgccgctc ccccgctctc
     1441 agctcgatca ccgtgccgag cacaccgagc ttgatcgtgg gaatcgcggc ctcgattgct
     1501 ttgacctcga gcacatcgcc ccagatcgtg agctcgagcg tgtggcctcg gtagcgcacg
     1561 agcacgtcga gccgctctac cggatccgga agccgcgggt tgatccagat gacaccttcg
     1621 cgcagctcga gtcccgtgta accgcgttgg atcaggtcga cgcttccggc catcgctccg
     1681 aggtggatgc cttcgcgtgt cgttccattc tggatgtcgc ggatgtcgct cgctagggca
     1741 tcccgagcga tctgccacga tccggcgcga cgcgaacgcg ccaacaccca cgcgtgcacg
     1801 acgcggctga gcgtcgagcc gtcactcgtg cggcgcaggt agtaatcgat cgtgcgcggg
     1861 atcgccgagg ggtcgaacgg atacccgagc cgctcgaaca acgtgcgcag ctcctcggag
     1921 gagaacaggt agtacagcat cagaacgtcc gcctgtttgg acgccttgta gcggttcggg
     1981 gaatcgccct cgctctcgag gatcagatcg aggcgttgga tgttgccgta gcgcgcgcgg
     2041 tagtccgccc aatcgagctc gaggagccga tcgtaacctt cgaactgggc gaggatgccg
     2101 ccgtcgagga acggcagcca catcttgcgg ctgatctcgt cccaccgcac gagctcgtcg
     2161 tcgcccagcg cgagcttctt gcacacatcg tggcggcgat cgcgcggcaa cgcgtccagc
     2221 acgtcgcgcg cgcgccacag catccagacg gacatgacgt tcgtgtacgc gttgttgtcg
     2281 agtcctggtt cgtgcgcgcc tggatacgcg gtgtggaact cgtctggtcc caccacgccg
     2341 cggatctcga accgtccccg ctcggggaca tacgtcgcga gactggcgac gaaccgcgcg
     2401 atctcgagga acatctccgc gccgatatcg ccgaggaagt cgacgtcgcc ggtgacctgg
     2461 aagtactgcc agacgttgta tgcgatcgcc gcgccgacgt gccgctgcag gtgcgtgttg
     2521 tccacgatcc agcggccaga gcgcggattg aagtattgca gcggcgtctc ctcgcgcccg
     2581 tcgctgccgc tctgccacgg gtacatcgcg ccgcgatagc cttcgcgccg cgcggcccgg
     2641 cgcgccgccg cgagccggcg atagcggtac atcagcagtg cgcgcgtgat gtcggccatg
     2701 cgcgtgttga gcaacgggaa gatgaacagc tcgtcccaga acacgtggcc gcgatacgcc
     2761 tcgccgtgca acccacgcgc cccgacgccg gcgtcgagat cggcggagtg ctccgatgcg
     2821 gtctgcagca agtgaaagac gtggagtctc aggatgacgc cggtcgacgg ttcgctggtc
     2881 cctgcgacct cgacgcaaat gtcgaatgcc cgccacaggc gctcccaggc gagcacgtga
     2941 gactcgacga gcgtcgcaaa agtaggtacc gccgcgatcc gctccgtcgc cgcgatgtcg
     3001 ggcgctgcga tcgcaaagtc acgcgacgtg tagatcgcga cgaccttctc gatccggatc
     3061 ggccgattct tcggcaattc gagcgagagc tcgcgtgctg cgaacccggg ccgggtgatc
     3121 gcggccgagc cgtgcgcgta gggtcgatcg ccatcgaaca cccgtgtgcg cgcggcctcc
     3181 gcgatgcgaa tgtgcgagcg cacggtctcc atctcgagaa gcacggagtc ttcgccgacc
     3241 gcgcgcgagt cgatcggtgc gaggtgttgc gacgccagta gtcggtagat ctcgacgccg
     3301 cggttctgca cccagccgtc gagcgcggct cggatctcga tcggtcccga ccagtcctcc
     3361 gcggtgagca cgacctcgag cgccgccaca tgcggatccg ccatgtgaac gatccacgtg
     3421 ctcgtgatgc gcgtcgtgca ccctttgcga tcgcgaaccc ggacgtcacg cgtgacaatc
     3481 ccgcgtcgca gatcgagcgt ctggcggtag ctcaggatct cggtcgtggc gagctcgaac
     3541 ggttcgccgc cggcgtgccg atacgtgagc agcagccagt tcggcatgtt gacgagatct
     3601 tcgtgctcga gcaggcggcc atcgatctcg gtcgataacc ggttgtagca acccgtgatg
     3661 tacgtcccgg cgtaatggac gcccccggcg gcgtcgtggg ccgcgccgcg tgtcgcgaag
     3721 tagccgttgc cgagcgtaca cagcgtctcg cgcacgcctt cctgcatcgg ctcgtagccc
     3781 tcgtagacga gctcccaccc cttcatcgca gctccatgcg cgtcgcatcg gacacgagct
     3841 cgatctcgtc gagcgacgag acgacgtgat cagcaccgct ctcgcgcagc gcttcaccat
     3901 ggccgttgcg atcgataccg atgacgaggc cgaaccgtcc tgcccgcgcc gcctgcacgc
     3961 ccgcaattgc gtcctcgaac acggccgtgc gtgcaacggg gacgccgagt cgccgcgccg
     4021 cctcgacgaa cgaatccgac gccggtttgc cccgcaggcc gagccgcgcg atctcgagcc
     4081 cgtcgacgcg cgcgtcgaac agccccgtga tccgtgccgc cgccagcacc gggccgccgt
     4141 tctcgcttgc agtgaccacc gccgtacgca cgccagacgc acgcgcccta cgcaacaacg
     4201 cgaccgcatc gccgtacacc tgcacgccat cgcgcgcgat gcgctcatgc acgtacttgt
     4261 tcttgcgctt cgcgagcgca tggatcgtct cggcgtcggc actgtcccct ggagtgcctt
     4321 cggggatcga gatgccacgc gccgcgagga agcctcgcaa cccatcgcgg cgcggcttgc
     4381 catctacgta tgcgcggtac tctcgatcgc tatcgaacgg cagccatgtc ctgcctgcgg
     4441 cgcgccgctc gagcaactcg tcgaacaact gcttccacgc ggccgcgtgg accgcagcgg
     4501 tgttcgtgac cacaccatcg agatcgagcg cgaatgcccg gtaaattgct aggtcgatcc
     4561 tcacgcagac ttggtgtcag gttctgcatc gcaggtgcca atcggcgccg gaaccgtggc
     4621 acccgcgccg gattcgcagt gtgccgcgct cggcaccaac gagaaacgcg ggctgagcgc
     4681 acaggaggcg cttacgtcgc gtgtaccggg cgtgcatggc gcgtcggctt cacgcggttg
     4741 cgcgcgagcc gccgctcgag ctctcgagcg agcacgaccg cgttgttgtg cacctcgtca
     4801 tgcgcggaat agatcagcgt gacgtttccg cgcgcggctc ggctcgcgag cttggcgagc
     4861 gcaaggcgcg caggcgcggc acgcagctct cgcaggtagc gttggcggaa ctcggagaac
     4921 cgcgtcggct cgtggccgaa ccacttgcga agctcgtggc tcggtgcgag ctccttgagc
     4981 cattcagcga tgtgcgcatc tgccttgcgc agcccacgcg gccatagccg ttcgacgagg
     5041 acgcgataac cgtcgctcgc ttctacgggt tcgtatgctc gcttcacccg gatcatgctc
     5101 ggatcgagag catcgttcgt gccacctcat ggcggctgcg gcagaattcc ttcgtagcga
     5161 tgggaatctg tgcgagagcg tgcacgcatc gctagcagcg ttccgcgaac atcacgccgg
     5221 caagccggtc cactcggcgg acttcgtcca cagctgcttt gcgagctctt cgtcgttcgc
     5281 gagcttgttc gggcggcgtt ccttgccgtc gcgatcgtag taacggccgg tctcgctcgc
     5341 gagctcgggc gcagtcgcgc agcgaatgct ggtctgcgca ccttcttcgg gcgtgaccat
     5401 gaacttcttc atcacccagc ggaccggacc gggcacgcgg cgccacacgt ccgtcgcgac
     5461 gacgccggga tgcagtgcgt aggtcgtgac ctggctgcct tcgaggcggc gcgcgagctc
     5521 cttcgtgaac agcacgttcg agagcttcga caccgcgtac tcgggcaggc ccgtgcgcga
     5581 cttcgtcttc gcgcgcaatg catcccagtt gatgccgcgc gcctgatagt gcgaggcgct
     5641 cgcgacgttg acgatgcgcg ccgggccgct ctccttgatc cgatcgagca acaggcgcgt
     5701 gaacagatag tggccgaggt ggttcgtgcc gaacgtcagc tcgaagccat ccttcgtcgt
     5761 gccgcgcgag cccgcgaggc ccgcgttgtt gatgaggccg tggatcggaa tcttacgggc
     5821 gagcagctct tccgcgcagc ggcgcaccga tgccagatcg gcgaggtcga gcgcgacgaa
     5881 ctcgatcttg tcattgccgg tttccttctt gatctcgtcg atgaccggct gggtcttgtc
     5941 cagcgagcga cacgcgagga tgacgtgggc gccgcgccgc gcgagctccc gtgccgtgat
     6001 tttgccgata ccggtgttcg cgccggtgat gacgaacgtc ttgccagcta ggtcgctcat
     6061 gtggggaaga acatgcgatc gcagcgcagg ccgtcaactc gcaaacgcca cgctcccgaa
     6121 accgtgggct ggcttgcaca ggtttcggtt gcgaaaccgg ctcctcattc gttttctaac
     6181 ggtcatttac ttgttgattg acccacgtcc atccgtcggg atacgagccc ggctgcatga
     6241 agcgactcgt cgtggtgctg gcgctatttg gtgtggtgct cgcatttggc ctcgcgtgcg
     6301 gggggaagcc gcaccgcgcg agtacggctg cgcacccgtc ggggccggtg ctttacgacc
     6361 gcatcggtcg catggacgcg atcaagggca tcgtcaaaga cttcgtcgag gagcagctca
     6421 agaagggcac gctcgcgggg cggttctcga atgtcgatac cgcgcagctc gaggacaacc
     6481 tcgcgcgtca gctgtgcgag ctgaccggcg gaccgtgcaa gtacacgggg cgcacgatgc
     6541 acgaggcaca cgccagcctg gcgatcacca gcgccgattt caccgcgttc gtcgcggcgt
     6601 tcgagcagtc actcgtgaag ttcaaggtcg agccgcagga gcagaacgag ctgctcgcgc
     6661 tctatcgcaa gcaacacgac gatatcgtca cgcagcctca gtagagcggg ccgccgctgt
     6721 tgccgccagg gccgtagtcg cagacgatca ttttgcgtcg ggatacgcga tcgcgaagat
     6781 cgcttctgcg gcgtcgagca tcggcaggcc cggatcgtcg aggagcgcac cgtcgatctc
     6841 gacgaagcca ctggagcttc ggcaggcctt cagctggtcg agaccgccga tccggcacag
     6901 ctgggcgccc attccatact tggtaacgat cagatcggga tcgaggcgga gcagctcttc
     6961 cgcggtgtat tgcggccagc cggtatacga ggtggcggcg acatcgacga ggccggctgc
     7021 accgaggacg tcaccgtagc tcgttccgcg cgtgccgccg aagaccttgc cgccatacgt
     7081 ggtgagtagc agggcgcgct tgcgtgcgcg gccatgcagt gcactggcga ccgctgcgaa
     7141 ccgccgctgc cacgcggtcg cgagccgttc gccgcgctcg gcgtgaccga gtagctgcgc
     7201 gacgatgtgg atgtcgcgca cgagcatgtc gacgccgccc atcgcgccga ggtcgaacac
     7261 cgcgatgcca cgctcgcgca gtcgcgcgac tttcgccgat tcaccgaagc tgttgagcag
     7321 caagagatcg ggagcgagcg acaacacggc ttcgacgtcg tcggtgctcg ccagcaccgg
     7381 cttgtccgct tgctggtagg cccacggcga ttgcgcccgt ccgtatcccg acacaccgac
     7441 tacgcgatcc ggttcggcga gctcgcggag cagccggtcg gcaacgaggc tgccggccac
     7501 gatcctgcga tacgccttga gctcgacgac ttgtccggtc gcatcgcgca atccgcggcg
     7561 gccgccaggc atctcgatga tctcgagaca cggagtcagc gcaggagcgg cggcgaggct
     7621 gcccatcggc gcgcggtatg acgctgcgac aagtgcgagg cccaccgcga ggacgacacc
     7681 gatcgtgttt gcgatcctga cgcgcgagct cacgtgactt ctcgaaagcc gagcgcgtcg
     7741 ccggggacga gctcgatgcc atacaccttg cgcaccggat ccctcgtgat gacgtcggcg
     7801 acaagcccac gtgccgccac actcccggcc tggagcagca ccgcaccgtc gcagtggcgg
     7861 cgtgcctcgt cgagatcgtg aagcacgaca acgaccgctg tcccggcggc cgcaagctcg
     7921 cgcgcgagtc ggaagaacgc gaggacgtga ccgatatcga gcgacgccgt cggttcgtcg
     7981 aacagcacga gtcgcgcgcc ggtggcgagc gcacgcgcga tcaacacgcg cttgcgttgg
     8041 ccgtgcgaga gctcgacgaa cgaacgatcc gcgaagtcga gagcatcggc gcggcgtagc
     8101 gcgtcctcga tcgcatcatg gtcggttgca ctgctccgcc cgaccccctg cgcatacgtg
     8161 tacctgccct gagcgaccac ctcgaatgcc gacagcggtg agtcgagacg ggactgttgc
     8221 ggcacatatc cgatgtggcg cgcacgtgcg cgcaggggca gacggtttac atccacacca
     8281 ccgacccaga tcgtaccttc gtacgctgcg agccctgcga tcgcacgaag cagcgttgtc
     8341 ttacccgcgc cattcgggcc gaggatcgcg gtgacggctc cgtgggctgc ggtgaacgac
     8401 gcgttcgcga ccgcgacgcg ggaggcgaac cggactgtga cgttatcgac gatgagtgca
     8461 tcacccatgc gcaatctccc gttgcgaacg caccacgagc accaagaaca gcgggccgcc
     8521 cacgagtccc gtgatcacgc cgagtggaag ctcgccgtga aacggtgcgg cgcggatcac
     8581 gacatcgcag agcaagagga acgcaccacc cgcgagcgca gccgcgggca gtaggcggcg
     8641 cggcgagatg ccgacgagtg gtcgcagcac gtgcgggacg acgaggccga cgaaaccgac
     8701 gttgccgccg atcgcgaccg cggccgatgt tccgacggcg acccagagca cggtccacca
     8761 ccgcacctgg cggacgtcga cgccgagtgt cgtggcctcg tcctgtccag acagcatcag
     8821 ctcgagcggc cggctccacg cccagagcgc gatgaacgcg gcgacgacga gcggcagccc
     8881 gatcagtacg tggcgaaggc ccgcgccacc gacgccaccg agcgtgaacg aaatgacggc
     8941 acgaccgagc tcccacgatt cctgcacgag gctgatcagc aacgcgccga cgctcacgaa
     9001 caacgacgac aacagaaaac cagtgagcag cactgcaagc tcgctccgcg tgtgctggat
     9061 gacggcgagc aggagcgtga gtgcgaagag tgcgccgatc aggcagccga gtggcagcac
     9121 catctccggc acgaggaacg cgggacgcag ccacgccggt gcgaacgaga aaccgaggat
     9181 cgcgagctgc ccaccgaggc tcgcacccgc ggtcgtgccg aggatcgcgg gatcgacgag
     9241 cggattgcgg aacaacgtct ggagcacgac gcctgcgacc gcgagtgctg cgcccgacaa
     9301 gaacgccgcg gctgcgcgca tgccacggag ctcgaggaag gtagagacca gcgcgcggtt
     9361 gccgagatca ccgcggccga caaagagcga gccgatgacg atcagcacgc tcgccgtcac
     9421 gatcaccgcg agccacgctc ggtacgactt catactcgac caccgttcgc gtcagtacca
     9481 ggcgatctcg cggggcggca gcccgatcgc cggattggca ttgatcgaag cttcgcgcgt
     9541 gatcgcggtc gcgtacgtgt agacatgcac tcgtggcatg gcggtcttcg catcggtcaa
     9601 gtacacgcgc tcgtggctcg ggtcgacgag caccgtgccg aacgtgaagc tgtcgctggc
     9661 atcggcgact ttcctggtgg tacccgcctg cacgttgaga gcccagaact gatcggtggg
     9721 gctgccgccg aagttgccga acgtgacgcc gaagccggtc gcgccctcgc gcgcgattcc
     9781 agaaaatccg gcgagcgcgc ggccgtcgaa tggtgtcatg tcctgcttgc gggtcatcgc
     9841 cggtggcgac gccgccgtgt cgacgtacgc gatgccggag ctcgccgctt gctcgggatc
     9901 gctgaatgat ccaccgcacg cgaccaccaa cgtcttcgta ctctccacgt acgagagtcc
     9961 gccgcagttc ttgaggcccg gtagatcgat cgtgccgctc acttgatcgg tagcggtatc
    10021 gaccacgacc aggcgcccct ccgccgccgt cgagaagtcg ccgctcaggt tcgacagcag
    10081 cacgaacagc ttgccgtcga tcagacgcgc gcggtctggg cgcgcctgga cgccgctgcc
    10141 ggcataactc gcgaggtcga tccgaccggt gatcgccggg accgagggat cgatgatcaa
    10201 caggtcatcg ccttcgtcgc gatccgcggg gtccgccgtc ggcgtggtgt tcttctcgta
    10261 acgtgcgacg tacgccttca tcgacgacac cgcgatcacg tcatgcgggt tcgcgtagaa
    10321 cccggtcgag acgtcgagct ggcgcagcgg cgtgcacgtg gtcggatcga tccacgtcag
    10381 cgcggaattc gcgtggtcga tcgtcacgag cagattcatc ggctgtggct gcgacggcag
    10441 cccgacgtct cccgaca
//
LOCUS       DASUFX010000601         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1209064, whole genome shotgun sequence.
ACCESSION   DASUFX010000601 DASUFX010000000
VERSION     DASUFX010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209064"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2095
                     /locus_tag="VFV99_24105"
     CDS             167..2095
                     /locus_tag="VFV99_24105"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5062486.1"
                     /translation="MIVLEILQGTATQRVHRVSGTSPVTLGRSPASTVTLTDFHLSGD
                     HAMISLVGEHYVFRDLRSTNGSAVERDGKRIAVDATTRWELALEDGDVLLLGNRNDPV
                     RLAVRIGEEADEDLGDRLVASRSIMDLPQVTDQIEGQPAEALRVYKALQPLGARLEPS
                     EVIEATVEATFELLPRATHVAILLRSEHDKDRFALAVSRQRPNHTPSQPIDPVRASRA
                     VLKRVLGDRAAVLTANAQQELLSSESIMAGQIMSMIAVPLWRGDDIIGLVQADNRQSA
                     GMFHERDLEVSLLLGAQAALAIDNATLVQRLRIAEEKARGENVYLKRREQKIKFDNII
                     GDSPAMKSVLAQLEKVIDTRATVCIEGETGTGKELIASAIHHQSSRRDKMFVAQNCAA
                     LPENLLESELFGHKRGAFTSADSDKKGLFEIADGGTLFLDEMGEMPMSLQAKLLRVLQ
                     EGTIRPVGATSEKQVDVRIICATNRDLSAEVEKGRFRQDLYYRLMVFPIRLPPLRDRR
                     EDISQLAAHFLKRYAEEYRIESPGFTQDALDALTSYNWPGNIRELENEIQRIVIQAES
                     GHWIEVTDLSPRLRKIEGTVTRIAPKQGTLKEMMEQVERWLIAEALRDHGGNKTKTAA
                     TLGITREGLHKKLAKFGV"
     gene            2140..2490
                     /locus_tag="VFV99_24110"
     CDS             2140..2490
                     /locus_tag="VFV99_24110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062487.1"
                     /translation="MRIVIALVLAGLVASGCAFDGAQRSSRAKPNVPAECRADTARDV
                     SIALFGLGMLGLGIASELQEPPLDAGNWLIVPGALLAITSTTSAIYGAVHGSTCQSAP
                     PQQRSGSANAAAMR"
     gene            2615..3031
                     /locus_tag="VFV99_24115"
     CDS             2615..3031
                     /locus_tag="VFV99_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062488.1"
                     /translation="MADAAVAVASLTAAYLLTIRAQSEGWGPYDFDRDGAIVLGGLGA
                     VTAASAAYGYGEVQGCEQLDHAWRDEQMATDTRRRTREHAQAAAWAATQQAAASARAG
                     DCATVTKLDADVRTLDPDFHATVFVRDVAIARCLGH"
BASE COUNT          546 a         1032 c         1025 g          486 t
ORIGIN      
        1 gaacgccggc acgccttccc gctcgaaccc gagctggacc tgccgtcctg cccgcgggtc
       61 cggatccagg acgatgactt tcgaagctcc cgaggacatg cgcgaagatc gagggtgaag
      121 tataaacctc tgaggggtca gcccagcggg ctaacttctt taccacgtga tcgttctcga
      181 gatcctgcag ggcacggcca ctcaacgcgt gcatcgcgtc agcggtacat cgccggtgac
      241 gctcgggcgc tcgcccgcga gcacggtcac gcttaccgat tttcatttgt cgggcgatca
      301 cgcgatgatc tcgctcgtcg gcgagcatta cgtattccgc gatctgcgct cgacgaacgg
      361 ctcggccgtc gaacgcgatg gcaagcgcat cgccgtcgat gcgaccacgc gctgggagct
      421 cgcgctcgag gacggcgatg tcctgctcct cggcaatcgc aacgatcccg tgcggctagc
      481 ggtgcggatc ggcgaggaag ccgacgagga cctcggtgat cgactcgtcg cgtcgcgctc
      541 gatcatggac ctgccgcagg tcaccgatca gatcgagggc cagccagccg aagcgctgcg
      601 cgtctacaag gcgctacagc cgctcggcgc acggctcgag ccgagcgagg tgatcgaggc
      661 gactgtcgag gcgacgttcg agctactccc gcgcgcgacc cacgtcgcga tcctcctgcg
      721 ctccgagcac gacaaggatc gcttcgcgct cgcggtatcg cgccagcgcc cgaatcacac
      781 gccgtcgcag ccgatcgatc cggtccgcgc cagccgcgcc gtgctgaagc gcgtgctcgg
      841 cgatcgcgcc gccgtgctca ccgcgaacgc gcagcaagag ctgctatcga gcgaatcgat
      901 catggccggt cagatcatgt cgatgatcgc cgtgccgctc tggcgcggcg acgacatcat
      961 cggtctcgtc caggcggaca accgccagtc cgccggcatg ttccacgagc gcgacctcga
     1021 ggtctccctg ctgctcggtg cccaagccgc gctcgcgatc gacaatgcga cgctcgtcca
     1081 gcgcctgcgc atcgccgaag aaaaggcacg tggcgagaac gtctacctca agcgccgcga
     1141 gcagaagatc aagttcgaca acatcatcgg cgactcgccg gcgatgaagt ccgtgctcgc
     1201 gcagctcgag aaggtgatcg acacgcgcgc gaccgtctgc atcgaaggcg agaccggcac
     1261 cggcaaagag ctgatcgcga gcgcgatcca ccaccagtcg tcgcgccgcg acaagatgtt
     1321 cgtcgcgcag aactgcgcag cgctgcccga gaacctgctc gagagcgagc tgttcggtca
     1381 caagcgcggc gcgttcacga gcgccgatag cgacaaaaag ggcctgttcg agatcgccga
     1441 cggcggcacg ctgttcctcg acgagatggg cgagatgccg atgtcgctgc aggccaagct
     1501 gctgcgcgtc ctccaggaag gcacgattcg tccggtcggc gcgacgagcg agaagcaggt
     1561 cgatgtccgc atcatctgtg cgacgaaccg cgacctctcc gccgaagtcg agaagggccg
     1621 cttccgccag gatctctact accgcctgat ggtgttcccg attcgcctgc cgccgctgcg
     1681 cgatcgccgc gaagacattt cccagctcgc cgcgcacttc ctcaagcgct acgccgagga
     1741 gtaccgcatc gaatcgcccg gcttcacgca ggacgcgctc gatgcgctga cctcgtacaa
     1801 ctggccgggc aacatccgcg agctcgagaa cgagatccag cgcatcgtga tccaggccga
     1861 atccggtcac tggatcgagg tgacggacct gtcgccgcgc ctgcgcaaga tcgaaggcac
     1921 cgtcacgcgc atcgcgccga agcaaggcac gctcaaagag atgatggagc aggtcgagcg
     1981 ctggctgatc gccgaagcgc tgcgcgatca cggcggcaac aagaccaaga ctgcagcgac
     2041 gcttggcatc acgcgcgaag gcctgcacaa gaagctggca aagttcggcg tctagcattt
     2101 cgccgtcggc aatcgcgccg ccgggtctat gatcgcgacg tgcggatcgt catcgcgctc
     2161 gtgcttgcgg gtctcgtcgc gagtggctgc gcattcgatg gagcgcagcg gtcgtcgcgc
     2221 gcgaaaccca atgtgcctgc cgagtgtcgc gccgatacgg cgcgcgacgt gtcgatagct
     2281 ctgttcggac tcgggatgct ggggctcggc atcgcgtcgg agctccaaga acctccactg
     2341 gatgccggca attggctgat cgtcccgggc gcgttgctcg cgatcacgtc gacgacatcg
     2401 gccatctacg gtgcggtcca cgggtcgacg tgtcagagcg cgccgcctca gcaacggagc
     2461 ggttctgcca atgcggcagc catgcggtaa tcgcggcacg caattcgccg tcgcgatccc
     2521 gctggcgcta agcgcgtgct cgttcggcgg cgtggcgacc gtgcgacgcg tagcatccga
     2581 tcgcgtcgag tgtgaggcgg actgggcgtt acccgtggcc gatgcggccg tcgcggtggc
     2641 cagcctgacg gccgcctact tgctgacgat ccgcgcgcaa agcgaagggt ggggcccgta
     2701 cgacttcgac cgtgatggcg caatcgtgtt gggcggcctc ggcgcggtga ccgctgcgtc
     2761 cgcagcctac ggctatggcg aggtccaggg ttgcgagcag ctcgatcatg cgtggcgtga
     2821 cgagcagatg gctaccgaca cgcggagacg gactcgcgag cacgcgcaag ccgctgcgtg
     2881 ggcagcgacg cagcaagctg cggcttcggc gcgcgcgggc gactgcgcaa cggtgacgaa
     2941 gctcgacgcc gacgtgcgga cgctcgatcc cgatttccat gcgacggtgt tcgtgcgcga
     3001 cgtcgcgatc gctcgttgcc tcgggcacta gccgcgcggg aggcccagga acgctgcata
     3061 gcgatcggcc gcggcgtcga cgagcttcg
//
LOCUS       DASUFX010000602        28935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1903531, whole genome shotgun sequence.
ACCESSION   DASUFX010000602 DASUFX010000000
VERSION     DASUFX010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 28935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28935
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903531"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFV99_24120"
     CDS             complement(<1..546)
                     /locus_tag="VFV99_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5062489.1"
                     /translation="MTTNSFSSKAHITVGGSKFDLYRLDALVKQKVGDVETLPYSLRI
                     LLENVLRYEDGKTVHAADVQAVASWNPKHRVEHEVQLRPARVLMQDFTGVPAICDLAA
                     MRDAFVKLGLPGSAINPLRAVDLVIDHSVQVDEYASPIAFKKNVELEYARNRERYLFL
                     RWGQQAFANTRVVPPGTGICHQ"
     gene            594..1832
                     /locus_tag="VFV99_24125"
     CDS             594..1832
                     /locus_tag="VFV99_24125"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX5062490.1"
                     /translation="MAPDEPTRQLTTEPAEVAEVVDAIRRAPLVAFDLEFVSQDRLVP
                     ALCLIQVAWLGEHVRLDAPALAIVAEVPHVRLVDPLSTDSRPIVEALAAHDLVIAHAP
                     RQDLALLATRFGTSMPGVVDTQLMAAFCGIGDQIGLASLGNDLLGLSLAKDMQWTDWE
                     KRPLSAAQLTYADADVRHLPALYAKLADRLGPRLEWVREESRAILDEAIAAANVTPET
                     AWQNVGGMRGLDETARAAVVVLAAWRQRVAAELDRPLGQVLPDKALVELAKVRPGNPG
                     GVRSVKGMSPIGKTRADEIALAIKTATATAAVPAERRMWRAPSARAQRWSELLLAIVQ
                     IVSEETGIAPRLLATRADAEEFARAVDEHGLEAAAALPALATWRRDVLGSVWEGWLAG
                     RISVIGEVAAPHGMRLLPRS"
     gene            1914..5159
                     /locus_tag="VFV99_24130"
     CDS             1914..5159
                     /locus_tag="VFV99_24130"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5062491.1"
                     /translation="MLACCSGEQSRLVSSTPAEVMAVPLTAATASIATSNSSHEDLVP
                     PWTLTASDGSGLMLTRVDARAVFEGPLAFTELHLYFHNPESRVREGTFQITLPQRAAV
                     SRFAMENNGQWMEAEVVEKQLARRAYDDFLHRRQDPALLEKAAGNQFTAKVFPIPANA
                     DKHIVISYSQELPGAPYTLPLRGLPKTERVDVSLALMSPDGKRSEQKLNERNWQPDRD
                     FVANASASAAAVMAGNLVAAQVEIGDRAAATEKPTSITLMIDTSASRALGFATYVGAV
                     RQLVDSLKSTYGADMPIQIVAFDQDAEQVYAGRAAGFGDEQAKKLFERGAAGASDLGQ
                     AFAWVAQHTPNQRVVVVTDGVVTAGAEGAELQKQIKGLAGAKVDRIDFVLAGGLRDEQ
                     AMTELVRSGLPHAGTVLDLDRDAVAVALGEAVKLDIEINVPGALWTYPKTIASARAGT
                     RTMVYARLAKPQQSIDVNVGGKVQKLAMLGGTAPLVERALASAEIDELEASLNKTTAP
                     NDARGLREAIAKKSVAARVISSQTSLLVLESDADFARYGIDRKSLADVLVVGANGIER
                     RGRTAPALIAQPQTATVTKKPPVNKDPWADRDVAGAKQEEAKDDKKTTGAGEDFDGRG
                     EAEKGNIAGGEVSGVEGGVVGGVVGGAPGGVAAGKPEPTVEAKPSVAPEAPPPAPPAS
                     VAQAQTSASRDAAERRLNNLHQDAAVRHRRVLEESERRQGLRISREQMENPLATDQPT
                     RRPAPDDGADEDDGDRGNREQWPPKDAPVALTGELAEINKLLKRDVDKALARARDWHA
                     REPGNVLALIGLGDALEAKGSKVTAARVYGSIIDLFPGRADMRRFAGERLARLQTQTQ
                     SQGAALIVDTYKRAVKDRPDHMTGHRLLAYALVRAGKHAEAFQAILTGVDQSYAGGRF
                     AGGERVLAEDAGMIGAAYAAAQPNKRAEIERELTKRNLSIATKPSTRFILYWETDAND
                     VDFHIQDAKGNHAFYSNPQLRSGGELYADITTGYGPECFAIQGTPTAGPYRVSINYYS
                     QGPMGYGMGLLEIQKFDGKGGLSFEDRPYVIMNDHAYVDLGLVANNQLASK"
     gene            complement(5163..5774)
                     /locus_tag="VFV99_24135"
     CDS             complement(5163..5774)
                     /locus_tag="VFV99_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062492.1"
                     /translation="MGLAMTRDDVVLQRPEDIRFLVPIFHTSNACCGAATAYLLRRIA
                     YRAGAYAPGIQEGMFHELNTSREGGTIEKVQHWFNGRVTSLHELGYRLQCRRVATPTS
                     ALLEWVKAGKGYRGAMLPTEFKRLHPEPNASGEDLDGDGVAHAVGITIDRLDAKADEE
                     LVMIDPWPGVKGGKERGAVHRQLELAHRARGFHALVYFWVGWS"
     gene            5977..6588
                     /locus_tag="VFV99_24140"
     CDS             5977..6588
                     /locus_tag="VFV99_24140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5062493.1"
                     /translation="MPRRPDLARRAELATAAFEVLRTRGVQTSMRELADALGVKRPTL
                     YFYFPDLGSVFESVLDQMYQEIAAFVMARVQAVDHPLDRLYAVVEATIDFHRQRPQLI
                     LSLLQLWSVTGRDFTKLLDRESRIVATSRAALVADLRAGIARKDVLPCDPDAVVGLVL
                     AVLSGVLVHQVLGIARPDGVTEELVARIIEPLRPKKKKARKRS"
     gene            6585..8288
                     /locus_tag="VFV99_24145"
     CDS             6585..8288
                     /locus_tag="VFV99_24145"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HEX5062494.1"
                     /translation="MTKPFFMARLAGTQAEMGTQHGHLVADDAVRLVEFYRTMPERAL
                     VGDMQGIAGKIGRSVARGLATAWQARLAKDRPAELAERSRAFMQAVLDSAPGAIAPRN
                     ALLTMATMDSLQNCVSLVARGKLGPFANPLSPRAAAAAMPACSTVIAWGAATHDGELM
                     FSRNFDFPGIGVWDAAPSFVVCAPTGGQRYGFFATRGADAPVVTCVNEAGLVIAPHTR
                     WHVGVKFGGAMIVDLIHDIARRAECLEDAIKIARERDTSSTWGIAVGSAREKSGLVLE
                     IAGPSVEVVRPAPGASYLVCANRYRSDAMQAGQVAATAAWAQHSERRERRLRALVDAR
                     TAPLAPETLARFMGDRHDVEAPQHRRRLGGILAQPTNVHCVVVSPAAQRALVGVDRAP
                     CCEGTWAELAWTWDGPTGGWEIGNTADTGFTAKLRTDVVAPHDAATLHVQDAARAYES
                     EHDIATARASLDKAIALDPEEPSLRLAAAWLAYEGGAYDRAMIHVHAGLARETESYRR
                     GQLLLWGSRAAQVIDPPQSKRWADDLAQLSGPHVEELKVASRQKYRRRPHVNLMMVDA
                     Y"
     gene            8357..8767
                     /locus_tag="VFV99_24150"
     CDS             8357..8767
                     /locus_tag="VFV99_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062495.1"
                     /translation="MGVLGGLLAAAALIISKKPDAKQYIDKLAAYQAIIGVGMIGVSI
                     VNFFRMLGGFTMTLKYLPLLGSSILGMLGTGVVLGALFGMPQIAKWIPGESSAEQKAV
                     ELSKKVAPYQVMLGVISVISSLIVLLYLLKILKP"
     gene            complement(8769..9758)
                     /locus_tag="VFV99_24155"
     CDS             complement(8769..9758)
                     /locus_tag="VFV99_24155"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5062496.1"
                     /translation="MLRFLGRTGVKVSRIALGAMSFGGDSDEATAGQIWRAARDAGIN
                     FVDTADVYNEGRSEEIVGRLMKGERDDIVLATKAYFPTGPGPNARGTSRYHLVRAVEA
                     SLRRLGTDRIDLYYLHRFDDATDIGETLRAVDDLVRAGKILYPACSNFAAWQVAHALG
                     VARAEHFAPLVAIQPMYNLVKRTAEVEILPMAQSLGIGVIPYSPTGGGLLTGKYGVGR
                     RPDKGRLIDWKMYETRYGIPTNFGVAEAFTQLAGELGHAPTTLAIAWVASHPAVTSVL
                     VGGRNLDQLAPAFAAKDVMLDETTRAKISALSPEPPPATDRNEEKTAHNFGSR"
     gene            complement(9758..10324)
                     /locus_tag="VFV99_24160"
     CDS             complement(9758..10324)
                     /locus_tag="VFV99_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062497.1"
                     /translation="MSEVTLEERGRALENQFYEKQDKEKLAAMKQKLDSQTTRDELRK
                     ASGMTDDAVLDKLVALGLRANTIAALSLVPLIEVAWADGEIQDNERTAILQGAHGKGL
                     EQGSDGYELLQSWLRKKPDSGLIEAWEAYIKALTGQLNDEQNKLLKNQIVGFAKMVAA
                     AAGGFLGIGKVSASEEKILHRIDAAFAR"
     gene            10433..11977
                     /locus_tag="VFV99_24165"
     CDS             10433..11977
                     /locus_tag="VFV99_24165"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HEX5062498.1"
                     /translation="MPMALLGGIALIAFASVYLLDHAVTGGAAGSAHDGALTRYVHFE
                     PGLISDAVAGLAAMTAAVLGIVITVVSLLVQLTSARYTGVAQMFLRDRTNVVVMAYYV
                     VTCVVGVALSLSLHSEFVPRAAVLTMMIACALGLVIMLPYFAYVFRFLEPVNLVTRIE
                     AEAAVDVRNAATAREQQTIIDAQRPAVAALEELTDITSNSISGKDKIIASRSVDAIKD
                     FIVGYLSVKNRADERWFKIGEGIRDNPDFVAMDPESLRTLEGDHTWLEWKALRQYLGI
                     YAEAQGSMPDINYLIAIDTRYVGEAALAKQDRHVVELAFRFMNSYLRAALNARAVRTA
                     YNVMNQYRLLVETMIRSGADDFAIEGVRHMIYYGRTSYDMQLGFVTETVAYDVSALCE
                     FAHSQKRDVDDKMLDMFLELDQPLRVKKQETGLQGIRKAQIKLACYYLAVGAIDRAKK
                     IADDMEGEDRERLDTIREQLTKVESKEFWEVIDRGRNFEYMPPRQKAQMERFFAMLPA
                     LAAPTS"
     gene            complement(11983..12765)
                     /locus_tag="VFV99_24170"
     CDS             complement(11983..12765)
                     /locus_tag="VFV99_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062499.1"
                     /translation="MECTRLACGVLLSGVIGSTALAQPEPVDNYFPDRLSGWRFELEP
                     FGLLPVDIDGTIQVGDRQVPFNADLGDVFDRLDFALNGRFEAWKQRWGIILNGQHYWL
                     GSEFTTPDGMTTFEHNTRIGVGSALIARRFGAYDINDRVSFSVDAEAGVWLGRIHSTL
                     EMSDGFSRTQNEIFPKAMLVARPILRITPSWAVINRNSITMPDISWMVMLGAEYDWRF
                     LAFELGYFAERIVLAKDVVTLAADAHGPYLGVAFRWGSGRPY"
     gene            complement(12859..13182)
                     /locus_tag="VFV99_24175"
     CDS             complement(12859..13182)
                     /locus_tag="VFV99_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062500.1"
                     /translation="MIAWLLSFVATCAVELAIVWWLGPPHRRRVPVAFVAQLGTHPIV
                     WLAMAALPGSQITRLASVELWATLVEAAVYKRFLGMRGRDAFALSALANAASLLAVAS
                     AAFVV"
     gene            complement(13179..14195)
                     /locus_tag="VFV99_24180"
     CDS             complement(13179..14195)
                     /locus_tag="VFV99_24180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062501.1"
                     /translation="MASVAANDVKAVAVDVGLASPLRVASTGVDTHRPRVALPARSAT
                     LVRVAGLAVLCAATLVAPALLLVWMPIVLGVPHIASDIRFLVLPLPRRQVVISLGACA
                     ALVALKTVSLASGVALVQSEMVVVGLWLVGLIALDRPTQRSHALQRHARVKRRSKLVW
                     PFVAFAAMTIVALPLQFAIVAAFAHNVVAVIAWVVVKRPGRRQALAMVAVLAAAVALL
                     IVAGPAVASVTGGASSPWLTVDKAAHVMFGGVPLPAARALMIAFTFLQAVHYAVWLDW
                     VPRGAPRMPTKPWLLVVAGTLVVLTAGLVDPVWARTTYLALATFHIYLELVVLAARGA
                     RRLQ"
     gene            complement(14486..16210)
                     /locus_tag="VFV99_24185"
     CDS             complement(14486..16210)
                     /locus_tag="VFV99_24185"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX5062502.1"
                     /translation="MSLHPPFREAAEAWLVEDTDPKTAAELRALIDRADKGDEAALRD
                     LREMFDGHLEFGTAGLRGILGPGPQRMNTVLVRKVTAGFATYLRANVPDAAKRGVVIG
                     HDARHNSRLFAEDTARVLAGAGIKAYLAHRPWPTPTTGWAVTALGAAAGVMVTASHNP
                     PAYNGYKVYWSNGAQIIPPHDKGIAAEIMKVGRSDQLPMPHLADARKKGLIVDLDENL
                     HDKYIAKVVALREKPALDGAGLIIAYTPLHGVGAASVEPALERAGFPRVHTEQSQRRP
                     DPDFPTVAFPNPEEKGAMDAVLELARRSNAEVVLANDPDADRLCVAVPDDGSYRVLTG
                     DQVGALLADYLLTVSTKDKRMVATTIVSSQLLSFLAEQAGADYRETLTGFKWIGNAAI
                     DYEKQTGGRFVMGYEEALGYSIGPLVRDKDGVSACVIFAELVVWNRARGKTVLDHLDD
                     IYRRVGLFVTEQVSLTKPGTDGLAEIRAAMTRFRAKPPGEIAGYKIEQVIDLSKGEGG
                     LPPSDVLVFKLAGGRRIIMRPSGTEPKLKSYYEVRVTIAAGEEVADARVRGVADLVKL
                     RDAHQALI"
     gene            complement(16313..16960)
                     /locus_tag="VFV99_24190"
     CDS             complement(16313..16960)
                     /locus_tag="VFV99_24190"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5062503.1"
                     /translation="MKRLVILLSLFVAAGAASAAPQGGGPTATAPAAKAGPGTAAVKT
                     ANEKISALLKQKPAAGSPQEKELAGKVTTSVRDFLDIDELGKRAMSDQWSKLTKEQQA
                     EFLSTLRALIEDNYVKGLRSNLAYTVDYTGETTDKDGNTIVTTTVNTKRKGRPFKINI
                     DYVLKKDGDKLKAWDVKTDGVGLVDNYRVQFNKIIEKDGFPGLIAKMKKKQSGAG"
     gene            complement(16970..18310)
                     /locus_tag="VFV99_24195"
     CDS             complement(16970..18310)
                     /locus_tag="VFV99_24195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5062504.1"
                     /translation="MRSLGIALVLIPALAAADPPKAKEPPKLTLDQVIAKAIANPKVQ
                     MAQSDSDMANARIKEADSAMYPKLKATAFGTASPHITCDDPGCTMTEPQNFAWDFKGV
                     YVSGQVDLTQPLYTFGKITHARNAARAGLDAQRALVNEAAGDAASDAARAYWGLKLAR
                     ELGYMLDDGIETIAKAQGNFDKRTDVTIQDRQRVAVLLAEAKARRAEAAQGEAQALAG
                     LRAITNTADADIDDAELVAVDKTLPAKNQLEDVAKQRPQSVAAEAGAHAYDELAKYQA
                     SYYFPDIAIVGFAFGAKAQGVTDPPGAFAYDPYNRIGAGLLLAMQWQIEPWTVKARTE
                     RARAEAHKMKAQSELATLGASYDAQTAVSEANAAKAKVGATAEGEKAARAWLASVLQN
                     EAIGTAEAKDLADAYIAWFQMKARWLEAVYQWNVAVVRVGRATGEFRAGAPRPR"
     gene            complement(18361..19224)
                     /locus_tag="VFV99_24200"
     CDS             complement(18361..19224)
                     /locus_tag="VFV99_24200"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX5062505.1"
                     /translation="MKLVWLVALAACACGKSHEPRVLDKQAAKGVFEEVPIDAPPGMS
                     DLTIDNRGVMWSIAERNPEVIEIELGKQPIRHPVDGVPKGTDTEALAWLDDSHFAIGT
                     EGASEATASIMFADKTDDHLVVTRTRPLTSAELGVELTINHGIEALCGNGDELLAASE
                     SVGKLPDGTRYSALARLRGDTVTVTKLRLTTAKGKISALYCAWGPDGSADVIAIERHY
                     SVSRILQFTVKRGDAEVTPTVMLDLAPILHDSLNLEGIVRLPDGRLVAISDNQGSAVV
                     GPTELLVFTKH"
     gene            complement(19221..20387)
                     /locus_tag="VFV99_24205"
     CDS             complement(19221..20387)
                     /locus_tag="VFV99_24205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5062506.1"
                     /translation="MLKYLFGVLLTGSFHLLVWWRLVKPAHLPKRAHRAITIAMIVLW
                     MSIPLTTTARLWAPGFASAASWITMPWMALIGLMTIAMIALELSRLVARAGRRAIGRR
                     EDEAQGDLMSRRKFLARVGSGAAVTAASTSVARGMIEARGEHEVVDVEVRLAKLPKAL
                     DGFSIVQLSDLHVGLTIDRGFVQRVVDHANRLSPDLIALTGDLVDGPVAELADEIAPL
                     ANLRAKHGVYAVTGNHEYYAGADPWIAAIKKLNVTYLRNRLVTIGDGAAAFDLAGVDD
                     HGAYKYSGHGEDLKAATANRDPSRALVLLAHQPRQVRRAAKHGVDLQLSGHTHGGQIW
                     PWHYIVKVQQGGLLAGRYEYEGTQLYVTRGCGYWGPPVRLLAPLEITRVILRSA"
     gene            complement(20424..21740)
                     /locus_tag="VFV99_24210"
     CDS             complement(20424..21740)
                     /locus_tag="VFV99_24210"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5062507.1"
                     /translation="MTSVPQVPGLPLIGNMLQFRRDRLDLQDRAARTGPIARIQLAHI
                     PIYIVTDADLAHQVLSEQAASFKKSAGLQFLKPLLGDGLLIAEGDDHKRHRKLLAPAF
                     APKRLEKYGDIMVAETEKQLARWRHGMRIDLANEMMEMTLAIAGRTMFSADVRGDAAQ
                     VAEALELGMRAQQANLRSPLQLGYEWPLPRHRQMRRAVKMLDEVVYRLIREGRARGTD
                     RGDVLSILLLASDEAGGLSDSQVRDEVMTLLLAGHETTANALTWTWFELGRHPDVLAK
                     LTLELEPFGRRPVAVEDLPKLPFNLAVIEEAMRVHPPVYMTGREACEDVDIGGHRLPR
                     GSIVAINIRGIHRRGDYFPAPDAFMPERMLPDAKKARPRHHYLPFGAGPRICIGSHFA
                     LLEAQLALATMVQHARLRLLATRVEPEPLVTLRPRGGMPAVVELRS"
     gene            21906..22469
                     /locus_tag="VFV99_24215"
     CDS             21906..22469
                     /locus_tag="VFV99_24215"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5062508.1"
                     /translation="MGTILEATSRVLVKQGFDGLSTNAVAAAAGVSIGSLYQYFPNKE
                     ALVAALIDRHMEEMNAAVLAELTRVAQLPIAEAARCVVELTIRAHAIDPDLHRVLTEQ
                     VPRIGKLARLREVDEICHRMVAGLLAARRDEIAIRDPDLAAFILVSTIEAVVHRAALL
                     YPHRLRDPRLVDETTLLVTRYLGVAEN"
     gene            22485..23462
                     /locus_tag="VFV99_24220"
     CDS             22485..23462
                     /locus_tag="VFV99_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062509.1"
                     /translation="MIRFALCSLVLALAACPKNGTTTQVQTGAGCPTAAGVYVASYAS
                     QDNGRSGWTLPLHAAPLQGDAPDYASIDAAAASAAGVPAAPTGALWLATAGAQPCQVK
                     VGGFYAAKIEGPPASVSYGVELDGCPAPKNPEEAGGVLLVSNESPTGCRFEVPQPIAG
                     RLGEMDKNKKWQPPTKESPLPKPLVDVLPQKQCAAPACETLWAFAQVTIDGKPVAWTG
                     AINWLQIGSEPDQCAWQAERSSGFWIPTDSSATKVTEGQEHSLPLIAVLADGGGAKVA
                     LAEGNGQYVTYDLSPGRAALGHRVEWMLAPPQAWDAIDHLGPICDEDRD"
     gene            complement(23459..24055)
                     /locus_tag="VFV99_24225"
     CDS             complement(23459..24055)
                     /locus_tag="VFV99_24225"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX5062510.1"
                     /translation="MLNRLDIETRDLHADVDGFWLDLMAAGVTREQYKNLLTRIYGFE
                     APIESALAYTPHLVVADRRERMRSGLIVQDLLALGITPGRITALPQCSAIGAFADPEE
                     ALGWKYVIERPTQLHSAIKRNIVGRIADVANACSYLSAYDGIVPARWQQFGILLDDVA
                     RRPNASERLVSAAKHAFSAMRDWFSRGAHEHVAHRDVS"
     gene            complement(24201..24449)
                     /locus_tag="VFV99_24230"
     CDS             complement(24201..24449)
                     /locus_tag="VFV99_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062511.1"
                     /translation="MPHNIAVNVGDHVFLEDGGEEIGAVRKVEKDHLVVYVEAAGDFV
                     VRGPEIKAVHYGKVVLKPELVDPRLLEAARTAHENESE"
     gene            24587..25096
                     /locus_tag="VFV99_24235"
     CDS             24587..25096
                     /locus_tag="VFV99_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062512.1"
                     /translation="MNPLRKLYNKLCRELAQSERFAATHVRREARRLGDTPPARALRA
                     LGGHAQAMCSRLDGVEQPPQPIGVLAGRKVGELFLVLRYFLFARLIHVERSYRGTLLG
                     FHESLSIGRLLRDVAERLDEQEMVGFCEQWLEQREPLLAIAERELHWFADTPRQAIRP
                     GFRMSALRE"
     gene            complement(25246..25413)
                     /locus_tag="VFV99_24240"
     CDS             complement(25246..25413)
                     /locus_tag="VFV99_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062513.1"
                     /translation="MQFIWPMAMPEFWYDAPSDTRDMAARERALSGHTSMLGIAVLVL
                     ALLVAALYVAH"
     gene            25534..27135
                     /locus_tag="VFV99_24245"
     CDS             25534..27135
                     /locus_tag="VFV99_24245"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062514.1"
                     /translation="MVERAPAASRKMVTSRAGWMGMARDEESRAYLQSRLVLMSKLMF
                     WSFIVLLGSMVALYAQYPKKEPDGNRQIYIFAAIGVVTLAVIWRGFLLRRQLKLEWLY
                     AIDFFYAAGTGSIFASAAYIASSLRLSAMVNLIWAILITFLRTIVVPSTGRRTAIAGT
                     LVFLPLMLAAVGLAITTPQELPPDAYVASALMISVTVVLLATIGSRVIYGLRRQADVA
                     MRLGQYTLDNKIADGGNGSVYRAHHALLRRPTAIKLMLPDRIGAETVDRFEREVQHMS
                     RLTHWNNVSVYDYGRNPDGMFYYAMEYLDGLNLENLVIDFGRQPADRVIAILRQVCAA
                     LNEAHRLGIIHRDIKPANIILCHRGDVPDVAKVVDYGLVKEITAEKGESMILGTPSYV
                     APEAVTDPATVGPAADLYAVGLVGYFLLTGRRAFEGNTTVDVCVQHVTAQPKSPSSHG
                     VKMPAELEQLILQCLAKQPSGRPADAGTLAKLLAAVPPAGDWSEDEAQRWWSDFRKLD
                     RPTAASADTLTITVDIEDRDEAGAA"
     gene            27233..27760
                     /locus_tag="VFV99_24250"
     CDS             27233..27760
                     /locus_tag="VFV99_24250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062515.1"
                     /translation="MRRIGLVVMAAFLVTANMAGAEPRRGLTSQPAKAARVKAPLAKP
                     TPPAPRSIKPVAKAAMPVNEPVAESTIDNPKPLPIPANALLMQYQRVGRDLLLLNNER
                     RAREGAENIDAKESCNELQTTFRSIKVEDAVKTPESRAAAAAVLAELHAKIVRLRGVE
                     LSQECLKNPLAKDCT"
     gene            complement(27757..28485)
                     /locus_tag="VFV99_24255"
     CDS             complement(27757..28485)
                     /locus_tag="VFV99_24255"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5062516.1"
                     /translation="MRTLSCLLAVTTFAGCTKWGTNTVYGPRHEVERRLLGEPSLATT
                     KSSSLNAGFAGAQGNGIAVAGLSGSTDSISRTHCVQQAEITYEQPFELEPVPKGRVND
                     VAGAVVLGFLGASVMIAAKVRSHTIFEPGDPLYEEPPSPTGGYLLGGAMIGTGIGLLA
                     YSFGSLPKGPKPAVQASTRRWQQVELVEATGCNLPSDPAVVQKAPANGDTAARLHQLD
                     QLRAAGTITEAEYQQRRRAILDSI"
     gene            28627..>28935
                     /locus_tag="VFV99_24260"
     CDS             28627..>28935
                     /locus_tag="VFV99_24260"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5062517.1"
                     /translation="MTNDDRKRSEQLAAQDTLAGYDRWAASYDAEPNALMSATAWVLD
                     RAPLGCADCDVLELGCGTGRNAQRVIAEGARSYTGLDGSQGMLAVAAQRYNDPRISFT
                     A"
BASE COUNT         4821 a         9449 c         9786 g         4879 t
ORIGIN      
        1 ctggtggcag atgccggtgc ccggcggcac gacgcgcgtg ttcgcgaacg cttgctggcc
       61 ccaacgcagg aagaggtagc gctcgcggtt gcgtgcgtac tcgagctcga cgttcttctt
      121 gaacgcgatc ggcgaggcgt actcgtcgac ctggaccgag tgatcgatga cgagatcgac
      181 ggcgcggagc gggttgatcg cgctgcccgg cagcccgagc ttgacgaatg cgtcgcgcat
      241 cgctgcgagg tcgcagatgg cggggacgcc ggtgaagtct tgcatcagca cgcgggccgg
      301 gcggagctgg acctcgtgct cgacgcggtg tttcgggttc caggacgcga ccgcctggac
      361 gtcagcagcg tggacggtct tgccgtcttc atagcgaagg acgttctcga gcaggatgcg
      421 cagtgagtag ggcagggtct cgacgtcgcc gaccttctgt ttgacgagtg cgtcgagtcg
      481 atagaggtcg aacttggagc caccgacggt gatgtgcgct ttgctggaga aactgttcgt
      541 cgtcataaag cctcgcgggg cgaatctaca ggtttcttgc gttatggtgt ggcgtggcgc
      601 ccgacgaacc aacgcggcaa ctcacgaccg agccggcgga ggttgccgag gttgtggacg
      661 cgattcggcg ggctccgctc gtcgcgtttg acctcgagtt cgtctcgcag gatcggctcg
      721 ttccggcgct atgcctgatt caagtggcgt ggctcggcga gcacgtgagg ttggatgctc
      781 ccgcactcgc gatcgtcgcg gaagttccgc acgtgcgact cgttgaccca ttgtcgacgg
      841 atagccggcc gatcgtcgag gcgctggccg cgcacgacct ggttatcgcg catgcgccgc
      901 gccaggatct tgcgctgttg gcgacgcgat ttggcacgtc gatgccggga gttgtcgata
      961 cgcagctgat ggctgcgttc tgtgggatcg gggaccagat cgggcttgcg tcattgggca
     1021 atgacctcct cgggctctcg ctcgcgaagg acatgcagtg gaccgattgg gagaagcggc
     1081 cgctgtcggc cgcccagctc acgtacgcgg atgccgacgt gcggcatctg ccggcactct
     1141 acgcgaagct ggccgatcgg ctcggcccgc ggctcgagtg ggtgcgtgag gagagtcgcg
     1201 cgatcctcga cgaagcgatt gccgcagcca acgtgactcc ggagactgcg tggcaaaacg
     1261 taggcggtat gcgcgggctg gatgaaacgg cgcgcgcggc cgtggtcgtg ctcgcggcgt
     1321 ggcggcagcg ggtggcggcg gagctcgatc ggccgctcgg ccaggtgctg cccgacaagg
     1381 cgctcgtcga gctcgcgaaa gtgcggcccg gcaatccggg cggcgtgcgg tccgtgaagg
     1441 gaatgtcgcc gatcggcaag acgcgcgctg acgagatcgc cctagcgatc aagacggcga
     1501 ctgcaactgc cgcggtgccg gcggagagac gaatgtggcg ggcgccgagc gcgcgtgcgc
     1561 agcggtggtc ggagctgctg ctcgcgatcg tgcagatcgt cagcgaggag accggcatcg
     1621 cgccgcgcct gctggcaacg cgcgccgacg cggaagaatt cgcccgcgcg gtcgacgagc
     1681 atggcttgga agcggcagcc gcgctgcccg cgctcgcaac ctggcgtcgc gacgtgctcg
     1741 gcagcgtgtg ggagggatgg ctcgcaggtc ggatttcggt gatcggcgag gtcgcggcgc
     1801 cgcatggcat gcggctcttg ccgcgcagtt agcacattcc gcaactctca gaacccgacg
     1861 cctgtccatg cgtttgaatg cgcatgccca gccggtcgtc tgtcctcctt ttgatgttgg
     1921 cctgttgctc gggagagcag agcaggctcg ttagctcgac acccgccgaa gtgatggcgg
     1981 tgccgctcac cgccgcgact gcgtcgatcg cgacgtcgaa ttcgtcgcac gaagacctcg
     2041 tgccgccgtg gacgctgacc gcgagcgacg gcagcggcct catgctcacg cgcgtcgacg
     2101 cgagggcggt gttcgaaggc ccgctcgcgt tcaccgagct gcatctgtat ttccacaacc
     2161 cggagagccg cgtccgcgag ggcacgttcc agatcacgct gccgcagcgc gcggcggtgt
     2221 cgcggttcgc gatggagaac aacggccagt ggatggaagc cgaggtcgtc gagaaacagc
     2281 tcgcgcgccg cgcgtatgac gacttcttgc atcgccgcca ggatccggcg ctgctcgaga
     2341 aggcggcggg caaccagttc accgcgaagg tgttcccgat ccccgcgaac gccgacaagc
     2401 acatcgtgat cagctacagc caggagctgc cgggcgcgcc gtacacgctg ccgctgcgtg
     2461 ggttgccgaa gaccgagcgc gtcgacgtgt cgctcgcgct gatgagcccc gacggcaagc
     2521 gcagcgaaca gaagctgaac gagcgcaact ggcagcccga ccgcgatttc gtcgcgaacg
     2581 cgagtgcgag cgcggcggcg gtgatggcgg gaaatctcgt ggcggcgcag gtggagatcg
     2641 gtgaccgcgc ggcggcgacg gagaagccga cgagcatcac gctgatgatc gatacgagtg
     2701 catcacgcgc actcggcttt gcgacatacg tcggtgcggt gcgccagctc gtcgatagct
     2761 tgaagtcgac gtatggcgcc gatatgccga tccagatcgt cgcgttcgat caggatgccg
     2821 agcaagtgta cgcggggcgc gcggctggct tcggcgacga gcaagcaaag aagctgttcg
     2881 agcgcggcgc ggccggtgcg tcggacctcg gccaggcgtt tgcgtgggtc gcgcagcaca
     2941 cgccgaacca gcgcgtcgtc gtcgtgaccg atggcgtcgt cacggccggc gcggaaggcg
     3001 cggagctgca gaagcagatc aagggtctcg cgggcgccaa ggtcgatcgc atcgacttcg
     3061 tgctcgccgg tggtctgcgc gacgagcagg cgatgaccga gctcgtgcgc agcggattgc
     3121 cgcacgccgg caccgtgctc gacctcgatc gcgatgccgt tgcggtcgcg ctcggtgaag
     3181 ccgtgaagct cgacatcgag atcaacgtgc caggcgcgct gtggacgtac ccgaagacga
     3241 tcgcgtcggc acgcgcgggc acgcgcacga tggtctacgc gcggctcgcg aagccgcagc
     3301 agtcgatcga cgtcaatgtc ggcggcaagg tgcagaagct cgcgatgctt ggcggcacgg
     3361 cgccgctcgt cgagcgtgcg ctcgccagtg ccgagatcga cgagctcgaa gcgtcattga
     3421 acaagacgac cgcgccgaac gatgcgcgcg ggttgcgtga ggcgatcgcg aagaagtcgg
     3481 tcgcggcgcg cgtgatttcg agccagacgt cgctactcgt gctcgagagc gacgccgact
     3541 tcgcgcgcta cggcatcgac cgcaagtcgc tcgccgacgt gctcgtcgtc ggcgccaacg
     3601 gcatcgagcg caggggccgc accgcgccgg cgctgatcgc gcagccgcag accgcgaccg
     3661 tgacgaagaa gccgccggtg aacaaggatc cgtgggccga tcgcgacgtc gcgggcgcga
     3721 aacaagaaga ggcgaaggac gacaagaaga ccaccggcgc cggcgaggac ttcgacggcc
     3781 gcggcgaggc cgagaaaggc aacatcgcag gcggcgaggt ctcgggcgtc gagggaggcg
     3841 tcgtaggcgg cgtcgtaggt ggtgcaccgg gaggtgtcgc tgccggtaag ccggagccga
     3901 cggtcgaggc gaagcccagc gtggcaccgg aagcgccgcc accggcgccg cctgcgtccg
     3961 tcgcacaggc acaaaccagc gcgtcgcgcg atgccgcaga acggcgccta aacaatctgc
     4021 atcaagacgc agctgtgcgg catcgtcgcg tcctcgaaga gtccgagcgc cggcagggcc
     4081 tgcgcatcag ccgcgaacag atggagaacc cgctcgccac ggatcagcct acgcggcggc
     4141 cggcgccgga tgatggcgct gacgaggacg atggcgatcg tggcaaccgc gagcagtggc
     4201 cgccgaagga cgcgccggtc gcgctcaccg gtgagctcgc cgagatcaac aagctgctca
     4261 agcgcgatgt cgacaaggcg ctcgcacgcg cacgcgactg gcatgcgcgc gagccgggca
     4321 acgtgctcgc gctgatcggc ctcggcgacg cgctcgaagc gaagggcagc aaagtgaccg
     4381 cggcgcgcgt gtacggttcg atcatcgatc tgttcccggg gcgcgccgac atgcgccggt
     4441 ttgcgggcga gcggctcgcg cgcctgcaga cgcagacgca gtcgcagggg gccgcgctga
     4501 tcgtcgatac gtacaagcgc gcggtgaagg accgccccga tcacatgacc ggccatcgct
     4561 tgctcgcgta cgcgctcgtg cgtgccggca agcacgccga ggcgttccag gcgatcctca
     4621 ccggcgtcga tcaatcgtat gcgggtggtc ggttcgccgg tggcgagcgc gtgctcgctg
     4681 aagacgcggg catgatcggt gcggcgtacg cggcagcgca gccgaacaag cgcgccgaga
     4741 tcgagcgcga gctcaccaag cgcaacctgt cgatcgcgac gaagccgtcg acgcgcttca
     4801 tcttgtactg ggagaccgac gcgaacgacg tcgacttcca cattcaggat gccaagggca
     4861 accacgcgtt ctactcgaac ccgcagctgc gctcgggcgg cgagctctac gccgacatca
     4921 cgacgggcta cggccccgag tgcttcgcga ttcaaggcac gccgacggca ggaccctatc
     4981 gcgtgtcgat caactactac tcgcagggcc cgatgggcta cggcatgggt ctgctcgaga
     5041 tccagaagtt cgacggcaag ggcggcttgt cgttcgagga tcgcccgtac gtgatcatga
     5101 acgaccacgc gtacgtcgac ctcgggctcg tcgcgaacaa tcagctcgcg agcaagtagt
     5161 cgttacgacc agccgaccca gaagtagacg agcgcgtgaa agccgcgcgc gcgatgcgcg
     5221 agctcgagct ggcgatgcac ggcgccgcgc tccttgccgc ctttgacacc cggccacgga
     5281 tcgatcatca cgagctcttc gtcggccttg gcgtcgaggc gatcgatcgt gatgccgacc
     5341 gcgtgcgcga cgccatcgcc gtcgagatcc tcaccggacg cgttcggctc gggatggagg
     5401 cgcttgaact cggtgggtag catcgcgccg cgatagccct tgccggcctt gacccactcg
     5461 agcagcgcgc tcgtcggcgt cgcgacgcgc cggcactgca ggcgatagcc gagctcgtgc
     5521 aggctcgtca cgcgcccgtt gaaccagtgc tggaccttct cgatcgtgcc gccttcgcgc
     5581 gaggtgttca gctcgtggaa catgccctct tgaatgccgg gcgcgtaggc gccggcgcgg
     5641 taggcgatgc ggcggaggag gtaggcggtc gcagcgccgc agcaggcgtt cgacgtgtgg
     5701 aagatcggga cgaggaaccg gatgtcctcc ggccgttgca agaccacgtc atcacgagtc
     5761 attgctagtc ccacgcgtgc ctcgcagcgt acacgaccgg cattggcgcg tgggacgcgc
     5821 gggccagcga tcgaggatcc ctcacagact tcatacggct ccggagggca tcggcttccc
     5881 agctccggag caatcggctc gccgactgcg gagcgatcgg ctccgggttt agattgacac
     5941 tatgtcagcc ttcttgtact gacactgtgt caggttatgc cccgccgccc cgacctcgcc
     6001 cgacgagccg agctcgcgac cgccgccttc gaggtgctgc gcacgcgcgg cgtgcagacc
     6061 agcatgcgcg agctcgccga tgcgctgggt gtgaagcggc cgacgctcta tttctacttt
     6121 cccgacctcg ggtcggtgtt cgagagcgtg ctcgaccaga tgtaccagga gattgccgcg
     6181 ttcgtgatgg cgcgcgtgca ggcggtcgac catccgctcg accggctgta cgcggtcgtc
     6241 gaagcgacga tcgacttcca ccgtcagcgg ccgcagctga tcctcagctt gctccagctg
     6301 tggtcggtga cagggcgcga cttcacgaag ctgctcgatc gggagagccg gatcgtcgcg
     6361 acgtcgcgcg ctgcgctggt cgccgatctc cgcgcgggca tcgcgcgcaa agacgtcttg
     6421 ccgtgtgatc cggacgctgt ggtcggtctc gtgctcgccg tgctcagcgg cgtgctcgtc
     6481 caccaggtgc tcggcatcgc gcgtcccgat ggtgtgaccg aggagcttgt cgcgcggatc
     6541 atcgagccgc tgcggccgaa gaagaagaaa gcgagaaagc gatcatgacg aagccgttct
     6601 tcatggcccg gttggcgggc acgcaagcgg agatggggac tcaacacggc catctcgtgg
     6661 ccgacgacgc ggttcgcctc gtcgagttct atcgcacgat gcccgagcgc gctctcgttg
     6721 gagacatgca gggcatcgcg ggcaagatcg ggcgcagtgt cgcgcgtggg ctcgcgaccg
     6781 cgtggcaagc gcggctggcc aaggatcgtc ctgccgagct cgccgaacgt tcgcgcgcgt
     6841 tcatgcaggc cgtactcgac agcgcacccg gcgcgatcgc gccgcgcaac gcgctgctga
     6901 cgatggcgac gatggactcg ctgcagaact gcgtgtcgct cgtcgcacgc ggcaagctcg
     6961 gcccgttcgc gaatccgctt tcgccgcgcg cggccgccgc tgcgatgccc gcgtgctcga
     7021 cggtgatcgc gtggggcgcg gcaacgcacg acggcgagct gatgttctcg cgcaacttcg
     7081 acttccccgg catcggcgtg tgggacgcgg cgccgtcatt cgttgtctgc gcaccgaccg
     7141 gtggccagcg ctatggcttc tttgcgacgc gtggcgccga cgcgccggtg gtcacgtgcg
     7201 tcaacgaggc gggcctcgtg atcgcgccgc acacgcgctg gcacgtcggc gtgaagttcg
     7261 gcggcgcgat gatcgtcgat ctcatccacg acatcgcacg ccgcgccgag tgtctcgagg
     7321 acgcgatcaa gatcgcgcgc gagcgcgaca cgtcgtcgac gtggggcatc gcggtcggct
     7381 cggctcgcga gaagagcggc ctcgtgctcg agatcgccgg cccgtcggtg gaggtcgtgc
     7441 ggcccgcgcc cggcgcgagc tacctcgtgt gtgcgaaccg ctatcgctcc gacgccatgc
     7501 aagcggggca ggtggccgct accgctgcat gggcgcagca ctccgagcgc cgcgaacgtc
     7561 gcttgcgcgc gctcgtcgat gcgcggaccg cgccgctcgc gccggaaaca ctcgctcgct
     7621 tcatgggcga tcgccacgac gtcgaagcac cgcagcaccg ccgtcgactc ggcggcattc
     7681 tcgcgcagcc gacgaacgtg cactgcgtcg tcgtgtcgcc ggccgcgcag cgcgcactcg
     7741 tcggtgtcga tcgcgcaccg tgctgcgagg gcacgtgggc cgagctcgca tggacgtggg
     7801 acggcccgac cggcggctgg gaaatcggca acaccgctga caccggcttc acggcgaagc
     7861 tgcgcaccga cgtcgtcgcg ccacacgacg ccgcaacgct gcacgtgcag gacgccgcgc
     7921 gcgcctacga atccgagcac gacatcgcaa ccgcgcgcgc ttcgctcgac aaagcgatcg
     7981 cgctcgatcc cgaggagccg tcgctgcgac tcgctgcggc atggctcgcg tacgaaggcg
     8041 gggcctacga tcgcgcgatg atccacgtgc acgcggggct cgcgcgcgag accgagagtt
     8101 atcgccgcgg ccaattgctg ttgtggggct cacgcgccgc acaggtgatc gatccgcctc
     8161 agtcgaagcg ctgggccgac gatctcgctc agctgtccgg ccctcacgtc gaggagctga
     8221 aggtcgcgtc tcgccagaag taccgaaggc gcccacacgt caacttgatg atggtcgacg
     8281 cgtattgata cgatttgccc ggccaaaggg ggcaatcatg ggagcttttg acgtactcgt
     8341 tttcgtcaac gtagtcttgg gcgttctcgg cggcttactc gcagctgccg cgctcatcat
     8401 ctcgaagaag ccggacgcga agcagtacat cgacaagctc gctgcgtatc aagcgatcat
     8461 cggcgtcggc atgatcggcg tctcgatcgt gaacttcttc cgcatgctag gcgggttcac
     8521 gatgacgctg aagtatctgc cgctgcttgg ctcgagcatc ctcggcatgc tcggcacggg
     8581 cgtcgtgctc ggcgcgctgt tcggcatgcc gcagatcgcg aagtggatcc ccggtgagtc
     8641 gtcggcggag cagaaggcgg tggagctgag caagaaggtg gcgccgtatc aggtcatgct
     8701 cggcgtcatc tcggtcatct cgtcgctgat cgtgctgctc tacctgctca agatcttgaa
     8761 gccgtagatc agcgcgaacc gaagttatgc gccgtcttct cttcgttgcg atcggtggca
     8821 ggcggcggct cgggtgacag cgcgctgatc ttcgcgcgcg tcgtctcgtc gagcatgacg
     8881 tccttggccg cgaacgccgg cgcgagctgg tcgaggttgc ggccgccgac gagcacgctc
     8941 gtcaccgccg gatgcgacgc gacccacgcg atcgcgagcg tcgtcggcgc gtggccgagc
     9001 tcaccggcga gctgcgtgaa cgcctcggcg acgccgaagt tcgtcggtat cccgtagcgg
     9061 gtctcgtaca tcttccagtc gatcaggcgg cccttgtccg gccggcgccc gacgccgtac
     9121 ttgccggtga gcaagccgcc accggtcggg ctgtacggaa tcacgccgat gccgagcgac
     9181 tgcgccatcg gcaggatctc gacctcggcg gttcgcttga ccaggttgta catcggctgg
     9241 atcgcgacga gcggcgcgaa gtgttcggcg cgcgcgacac cgagcgcgtg cgcgacctgc
     9301 caggctgcga agttcgagca cgccggatac aggatcttgc ccgcgcgcac cagatcgtcg
     9361 acggcgcgca gcgtctcgcc gatgtcggtc gcatcgtcga agcggtgcag gtagtagagg
     9421 tcgatgcgat cggtcccgag gcgccgcagg ctcgcctcga ccgcgcgcac caggtggtag
     9481 cgcgaggtgc cgcgcgcgtt cggacccggg ccggtcggga agtaggcctt cgtcgcgagc
     9541 acgatgtcgt cgcgctcgcc cttcatcagc cgacccacga tctcttcgga tcggccctcg
     9601 ttgtagacgt cggccgtgtc gacgaagttg atgccggcat cgcgcgccgc gcgccagatc
     9661 tggccggcgg tcgcctcgtc ggaatcaccg ccgaacgaca tcgcgccgag cgcgatgcgc
     9721 gacaccttga ccccggttct gccgaggaag cgcagcatca gcgggcaaac gcggcgtcga
     9781 tgcggtggag gatcttctcc tccgatgccg acaccttgcc gattccgagg aacccgcccg
     9841 cagcggccgc gaccatcttc gcgaacccga cgatctgatt cttgagcagc ttgttctgct
     9901 cgtcgttgag ctggccggtg agcgccttga tgtacgcctc ccaggcctcg atcagccccg
     9961 aatcgggctt cttgcgcagc cagctctgga gcagctcgta gccgtcgcta ccctgctcga
    10021 ggcccttgcc gtgcgcgccc tggaggatgg cggtgcgctc gttgtcctgg atctcaccgt
    10081 cggcccatgc gacctcgatg agcggcacca gcgacagcgc agcgatggtg ttcgcgcgga
    10141 ggccgagggc gaccagcttg tcgaggaccg cgtcgtccgt catgccggag gccttgcgga
    10201 gctcgtcccg cgtggtctgc gagtcgagct tctgcttcat cgctgcgagc ttctccttgt
    10261 cctgcttctc gtagaactgg ttctcgagcg cgcggccgcg ctcttcgagg gtcacttcgc
    10321 tcacgacggg ctccttgttg agagctggga ccttatcaaa tatcgtgctg aggtgacacc
    10381 acgggtacgg accaagcgga gagcgcatcg cgcgggtgcg cgcctatggc tcgtgccaat
    10441 ggcgctcctc ggcggaatcg cgctgatcgc ctttgcatcg gtgtacttgc tggaccatgc
    10501 ggtcaccggt ggcgccgctg gcagcgcgca tgatggtgcg ctgacccggt acgtccactt
    10561 cgagccgggg ctgatttccg acgccgttgc cggcctcgcg gcgatgaccg cagccgtgct
    10621 cggcattgtg atcaccgtcg tctccctcct cgtccagctg accagcgcgc gctacaccgg
    10681 cgtcgcgcag atgttcttgc gcgatcgcac gaacgtggtc gtgatggcgt actacgtcgt
    10741 cacctgcgtc gtgggcgtcg cgctgtcgct gtcgctgcac tccgagttcg tgccgcgcgc
    10801 ggccgtgctg acgatgatga tcgcgtgcgc actcggcctc gtgatcatgc tgccgtactt
    10861 cgcatacgtg ttccgcttcc tcgagccggt caacctggtt acccgcatcg aggcagaggc
    10921 ggccgtcgac gtgcgcaacg ccgccaccgc gcgcgaacag cagacgatca tcgacgcgca
    10981 acgccccgcg gtcgccgcgc tcgaagagct caccgatatc acgtcgaact cgatctcggg
    11041 caaggacaag atcatcgcga gtcgctcggt cgatgcgatc aaggacttca tcgtcggcta
    11101 tctgtcggtg aagaaccgcg ccgacgaacg ctggttcaag atcggcgagg gcatccgcga
    11161 caacccggac tttgtcgcca tggatccgga atcgctgcgc acgctcgaag gcgatcacac
    11221 gtggctcgag tggaaggcgc tgcgccagta cctcgggatc tacgccgagg cgcagggcag
    11281 catgcccgat atcaactacc tgatcgcgat cgacacccgg tacgtcggcg aggccgcgct
    11341 cgcgaagcag gaccgccacg tcgtcgagct cgcattccgg ttcatgaaca gctacctgcg
    11401 tgctgcgctc aacgcgcgcg ccgtgcgcac cgcgtacaac gtgatgaacc agtaccggct
    11461 gctcgtcgag acgatgattc gcagcggcgc cgacgacttc gcgatcgagg gtgtgcgcca
    11521 catgatctat tacgggcgca cgagctacga catgcagctc ggcttcgtga ccgagaccgt
    11581 cgcctatgac gtctcggcgc tgtgcgagtt tgcgcactcc cagaagcgcg acgtcgacga
    11641 caagatgctc gacatgttcc tcgagctcga tcagccgctc cgcgtgaaga agcaggagac
    11701 cggcctccag ggcatccgca aggcgcagat caagctcgct tgctattacc tcgcggttgg
    11761 cgcgatcgat cgcgcgaaga agatcgccga cgacatggaa ggcgaagatc gcgagcgcct
    11821 cgacacgatt cgcgagcagc tcacgaaggt cgagtcgaag gaattctggg aagtcatcga
    11881 ccgcggccgc aacttcgaat acatgccgcc gcggcagaag gcgcagatgg agcgcttctt
    11941 cgcgatgctg cccgcacttg ccgcgccgac gagctaaccg gcctagtacg gacgtcccga
    12001 gccccagcgg aaggcaacgc cgaggtaggg accgtgtgcg tcggcggcga gcgtgacgac
    12061 gtccttggcg agcacgatcc gctccgcgaa atacccgagc tcgaacgcga ggaaccgcca
    12121 gtcgtactcg gcgccgagca tcaccatcca gctgatgtcc ggcatcgtga tcgagtttcg
    12181 gttgatgacc gcccacgacg gcgtgattcg caagatcggg cgcgcgacga gcatcgcctt
    12241 ggggaagatt tcgttctggg tgcgcgagaa cccatcggac atctcgagcg tgctgtgaat
    12301 gcgcccgagc catacgccgg cctcggcgtc gacggagaac gaaacgcggt cattgatgtc
    12361 atacgcgccg aagcgacgcg cgatcagcgc gctgcccacc ccgatccgcg tgttgtgctc
    12421 gaacgtcgtc atcccgtctg gagtcgtgaa ttcactgccg agccagtaat gctgtccgtt
    12481 gaggatgatg ccccaccgct gcttccacgc ctcgaagcga ccgttgagtg cgaagtcgag
    12541 gcggtcgaac acgtcaccga gatcggcgtt gaacggaacc tggcgatcac cgacctgaat
    12601 ggttccgtcg atgtcgactg gaagcaggcc gaatggctcg agctcgaagc gccatcccga
    12661 gagccggtcc ggaaagtagt tgtcgaccgg ttccggctgc gccaacgccg tgcttccgat
    12721 gacgccactc agcaacactc cgcaagccag acgcgtgcac tccatgcgat ggagctttga
    12781 aggcgatcgg ggggctgaca agcaaaacgc ccgtcttgcc catctcacta ccgtcgaacc
    12841 cgtgtggcag agcgctcgct acacgacgaa tgccgccgac gcgaccgcaa gcagcgatgc
    12901 cgcattcgcg agcgccgaca gtgcaaatgc gtcgcggccg cgcataccga gaaaccgctt
    12961 gtacacggcc gcctcgacga gcgttgccca tagctcgacc gatgcgaggc gcgtgatctg
    13021 cgagccgggc agggcggcca tcgcgagcca cacgatcgga tgcgtgccca gctgcgcgac
    13081 gaacgcgact ggcacgcggc ggcgatgcgg cggaccgagc caccagacga tcgcgagctc
    13141 gactgcacac gtcgcgacga acgagagcaa ccaggcgatc actgaagtct ccgcgcgcca
    13201 cgcgcggcga ggacgacgag ctctagatag atgtgaaacg ttgcgagcgc gaggtacgtg
    13261 gtgcgcgccc acaccggatc gacgagccct gcggtgagca cgacgagcgt accggcgacg
    13321 acgagcagcc acggcttcgt cggcatgcgc ggtgcgccac gcggaaccca gtcgagccac
    13381 accgcgtagt gaaccgcttg cagaaacgtg aacgcgatca tcagcgctcg cgcggccggc
    13441 aacgggacgc cgccgaacat gacgtgtgcc gctttgtcga cggtgagcca cggcgagctc
    13501 gcaccaccgg tgacgctcgc gacggcaggc ccggcgacga tcaacagcgc gacggcagcg
    13561 gcgagcacgg cgaccatcgc gagtgcctgg cggcgccccg gccgtttgac gacaacccac
    13621 gcgatcaccg cgacgacgtt gtgggcgaac gcggcaacga tcgcgaactg cagcggcaac
    13681 gcgacgatcg tcatcgccgc gaacgcgacg aacggccaca cgagcttcga acgccgcttc
    13741 acgcgtgcgt gacgctggag cgcgtgcgaa cgctgggtcg ggcggtcgag cgcgatcaga
    13801 ccgacgagcc acaacccgac gacgaccatc tcggactgca cgagcgcgac gccgctcgcg
    13861 agcgacacgg tcttgagcgc cacgagcgcg gcacacgcac cgagcgagat cacgacctgg
    13921 cggcgcggca gcggcagcac gaggaaccgg atgtcgctcg cgatgtgcgg aacgccaagc
    13981 acgatcggca tccacacgag cagcagcgcc ggtgcgacga gcgtcgcagc gcacagcacc
    14041 gcgaggccgg cgacccgcac aagtgtcgcc gacctcgcgg gcagtgcaac ccgaggccgg
    14101 tgcgtgtcga cgccggtgct cgccacgcgc agcggcgaag cgagcccgac gtcgacggcg
    14161 accgccttaa cgtcgtttgc ggcgaccgag gccatacgca accgttccga tcatcaagag
    14221 gccgctgccg accgatgcgc tgtccgcgcc ggtggctctg caactgctat cgggtccact
    14281 gcaatggatc caaccgtcgg cgcgtgcgag ctgcggcgct gcggcaagaa caagcaaact
    14341 caggagcagc gtcttcatca ccacggtgcg ttgcagcttg tggtccgccc gggcacttcg
    14401 cgcgtttgcc acgtgctgcc gcgatctcca gtcaagctcc aaccgctggc aggtgaagct
    14461 tgaacgtggt gtcgagaagc cggcgtcaga tcagcgcttg atgcgcgtcg cgcagcttca
    14521 cgagatcggc aacaccacgc acgcgcgcgt cggcgacttc ctcgccggct gcaatcgtga
    14581 cgcgtacctc gtagtagctc ttcagcttcg gctcggtgcc gctcggccgc atgatgatcc
    14641 gccgcccacc ggcgagcttg aacacgagca cgtccgacgg tggcaagccg ccttcgccct
    14701 ttgacagatc gatcacttgc tcgatcttgt agccggcgat ctcgcccggt ggcttcgcgc
    14761 ggaaccgcgt catcgctgcg cggatctcgg cgagcccgtc ggtgccaggc ttcgtcagcg
    14821 atacctgctc ggtcacgaac agaccaacgc ggcggtagat gtcgtcgagg tgatcgagca
    14881 ccgtcttgcc gcgcgcacga ttccacacga cgagctcggc gaagatgaca cacgccgata
    14941 caccatcctt gtcgcgcacg agcggcccga tcgagtagcc gagcgcctcc tcgtagccca
    15001 tcacgaagcg gccgccggtc tgcttctcgt agtcgatcgc ggcgttgccg atccatttga
    15061 agccggtgag cgtctcgcgg taatcggcgc cggcctgctc ggcgaggaac gacaacagct
    15121 gcgacgacac gatggtcgtc gcgaccatgc gcttgtcctt cgtgctgacc gtcaacagat
    15181 agtcggcgag cagcgcaccg acctggtcgc cggtcaacac gcgatacgaa ccgtcgtcgg
    15241 gaacggcgac gcacaaccga tcggcatccg ggtcgttcgc gagcacgacc tccgcattac
    15301 tgcggcgcgc gagctcgagc acggcgtcca tcgcgccctt ctcctcggga ttcgggaacg
    15361 cgacggtcgg aaagtccgga tcgggccggc gctgcgattg ctcggtgtgt acgcgcggaa
    15421 agcccgcgcg ctcgagcgcc ggttcgaccg aagccgcgcc gacgccgtgg agcggcgtgt
    15481 acgcgatgat gagccccgcg ccatcgagcg ccggcttctc gcgcagcgcg acgacctttg
    15541 cgatgtactt gtcgtgcaga ttctcgtcga gatcgacgat gagccccttc ttgcgcgcgt
    15601 cggcaaggtg cggcatcggc agctggtcgc tacgccccac cttcatgatc tcggcggcga
    15661 tgcccttgtc gtgcggtgga atgatctgcg cgccgttgct ccagtagact ttgtagccgt
    15721 tgtatgcggg cgggttgtgg ctcgccgtga ccatcacgcc ggccgccgca ccgagcgcgg
    15781 tgaccgccca gcccgtcgtc ggcgtcggcc acggccggtg cgcgagatac gccttgatgc
    15841 cggcacccgc gagcacgcgc gcggtgtctt cggcgaacag ccgcgagttg tgccgcgcgt
    15901 cgtggccgat cacgacacca cgtttcgcgg cgtcgggcac gttcgcgcgc aggtacgtcg
    15961 cgaacccggc cgtcaccttg cgcacgagca cggtgttcat gcgctgcggt ccggggccga
    16021 ggatgccgcg caggcccgca gtgccgaact cgagatgacc gtcgaacatc tcgcggaggt
    16081 cgcgcagcgc ggcctcgtcg cctttgtcgg cgcggtcgat cagcgcgcgc agctcggcgg
    16141 ccgtcttcgg gtcggtgtct tcaacgagcc aggcttcggc agcttctcga aagggcggat
    16201 gcagcgacac cgctggacca tacccaaatc tctcgcgcgt ggcgcatcga gagcgcgcct
    16261 gcgtgcatga cgggcgatca cgggcaacgc cgtgcgcccg cgcccactcc gctcagcccg
    16321 cgccgctctg cttcttcttc atcttcgcga tgagacccgg gaagccgtct ttctcgatga
    16381 tcttgttgaa ctggacgcgg tagttgtcga cgaggccgac accgtcggtc ttgacatccc
    16441 acgccttgag cttgtcgccg tccttcttga ggacgtagtc gatgttgatc ttgaacgggc
    16501 ggcctttgcg cttcgtgttc acggtcgtcg tgacgatcgt gttgccatcc ttgtcagtgg
    16561 tctcaccggt gtagtcgacc gtgtacgcga ggttcgagcg cagccccttc acgtagttgt
    16621 cctcgatcaa cgcgcgcagc gtgctgagga actccgcctg ctgctccttc gtcaacttcg
    16681 accactgatc cgacatcgcg cgcttaccga gctcgtcgat gtcgaggaag tcgcgcaccg
    16741 aggtcgtgac cttgccggcg agctctttct cctgcggaga gccagccgcg ggcttctgct
    16801 tcagtagcgc cgagatcttt tcgttcgccg tctttaccgc cgccgtgccc ggaccggcct
    16861 tcgccgccgg ggcagttgca gtggggccgc cgccttgcgg ggcagccgaa gcggctcccg
    16921 ccgcgacgaa cagggacagc aaaataacca gacgtttcat gggcagttct tacctcggac
    16981 gtggggcgcc cgcccggaat tcaccggttg cgcgaccgac acgtaccaca gcgacgttcc
    17041 attggtagac ggcttctaac caccgggcct tcatttgaaa ccacgcgata tatgcatcag
    17101 cgagatcctt ggcttcagcg gtgccaattg cttcattttg caagaccgat gcaagccagg
    17161 ctcttgccgc tttttcccct tcggcggtag ccccgacctt ggccttggcg gcattcgcct
    17221 cggacacggc ggtctgggcg tcgtaactcg cacctagggt cgcgagctcg gattgcgcct
    17281 tcatcttatg tgcttcggcg cgcgcgcgct ccgtacgtgc cttcacggtc cacggctcga
    17341 tctgccattg catcgcgagc aggagcccgg cgccgattcg gttgtacggg tcataagcga
    17401 acgcacctgg tggatccgtg acaccttgtg cctttgctcc gaacgcaaat ccgacaatcg
    17461 cgatgtcggg gaagtagtag gacgcctggt acttggcgag ctcgtcgtac gcatgcgcac
    17521 ctgcctccgc cgccacgctc tgcggccgtt gcttcgccac atcctcgagc tggttcttcg
    17581 cgggcagcgt cttgtcgacc gcgacgagct cggcatcatc gatgtcggcg tccgcggtgt
    17641 tcgtgatcgc gcgtaatcca gcgagtgcct gcgcttcgcc ttgtgccgct tccgcgcgcc
    17701 gcgctttcgc ctcggcgagg agcaccgcga cgcgctggcg atcctggatc gtgacgtccg
    17761 tacgcttgtc gaaatttccc tgcgccttcg cgatcgtctc gatgccgtcg tcgagcatgt
    17821 agccgagctc gcgagcgagc ttgaggcccc agtacgcccg cgccgcatcc gacgcggcat
    17881 cgccggcggc ttcgttcacg agcgcgcgct gcgcgtcgag accggcgcgt gccgcattac
    17941 gggcgtgcgt gatcttgccg aacgtgtaca gcggctgcgt cagatcgacc tggccgctga
    18001 cgtacacgcc tttgaagtcc caggcgaagt tctgcggctc ggtcatcgtg cagccagggt
    18061 cgtcgcacgt gatgtgcggg ctcgcagtgc cgaacgccgt ggccttgagc ttcggataca
    18121 tcgcggagtc ggcttccttg atgcgggcgt tcgccatgtc gctgtcgctc tgcgccatct
    18181 ggaccttggg gttcgcgatt gccttggcga tgacctggtc gagcgtgagc ttcggcggct
    18241 cctttgcctt cggtggatcc gcggcggcga gggcagggat caagacgagc gcgataccga
    18301 gcgagcgcat ggcggggttg tacaccggcg ggcgcgacgg ggctcgcttc ccgatcgtcg
    18361 ctaatgcttg gtgaacacga gcagctcggt cgggccgacc acggcgctgc cttggttatc
    18421 gctgatcgcg acgagccggc cgtcgggcaa cctgacgatc ccttcgaggt tcagcgagtc
    18481 gtgcaggatc ggcgcgagat cgagcatgac cgtcggcgtg acctcggcat cgccgcgctt
    18541 gaccgtgaat tgcagaatac ggctgacact gtagtgccgc tcgatcgcga tcacgtcagc
    18601 gctcccgtcg gggccccacg cgcagtagag cgccgagatc ttgcccttgg cggtcgtgag
    18661 ccgcagcttc gtgaccgtga ccgtgtcgcc gcgcaaccgc gcgagcgccg agtagcgcgt
    18721 gccgtcgggc agcttaccga ccgattcgct cgccgcgagc agctcgtcgc cgttgccgca
    18781 caatgcctcg atgccgtgat tgatcgtgag ctcgacgccg agctcggcgc tcgtcagcgg
    18841 ccgcgtgcgc gtcacgacga ggtgatcgtc cgtcttgtcg gcgaacatga tcgacgccgt
    18901 cgcctcgctc gcgccctcgg tgccgatcgc gaagtggctg tcgtcgagcc acgcgagcgc
    18961 ttcggtgtcg gtgcctttcg gcacgccgtc gaccgggtgg cggatcggtt gcttgccgag
    19021 ctcgatctcg atcacctcgg gattgcgctc cgcgatcgac cacatcacgc cgcgattatc
    19081 gatcgtgaga tcggacatcc cgggcggcgc gtcgatcggc acctcttcga acacgccctt
    19141 cgcggcttgc ttgtcgagca cgcgcggctc gtggctcttg ccgcaggcgc acgcggcgag
    19201 agcaacgagc cagaccaatt tcatgcgctg cgtagtatca cacgcgtgat ctcgagcgga
    19261 gctagcaagc gcaccggcgg gccccagtag ccgcacccgc gcgtcacgta gagctgggtg
    19321 ccctcgtact cgtagcggcc ggcgagcagg ccaccctgct ggaccttgac gatgtagtgc
    19381 cacggccaga tctggccgcc gtgcgtgtgc cccgacagct ggagatcgac accgtgcttc
    19441 gcggcgcggc gcacctggcg cggctgatgc gcgagcagga cgagcgcccg gctcggatcg
    19501 cggttcgcgg ttgcggcctt caagtcttcg ccgtgaccgc tgtacttgta cgcgccgtga
    19561 tcgtcgacgc cggcgagatc gaacgcagca gcgccgtcgc cgatcgtgac gagccggttg
    19621 cgcaggtacg tcacgttgag cttcttgatc gccgcgatcc atggatcggc gcccgcgtag
    19681 tactcgtggt tgccggtgac ggcgtacaca ccgtgcttcg cacggaggtt tgcgaggggc
    19741 gcgatttcgt cggcgagctc ggcgactggg ccatcgacga ggtcaccggt gagcgcgatg
    19801 aggtcgggcg acagccggtt cgcgtgatcg acgacgcgct ggacaaaccc gcgatcgatc
    19861 gtgagcccga cgtggaggtc ggagagctgg acgatagaga agccgtcgag tgccttcggt
    19921 agctttgcga gcctgacctc gacgtcgacg acctcgtgct cgccacgcgc ttcgatcatg
    19981 ccgcgcgcga cgctggtgct cgcagcggtg accgcggcgc cactgccgac gcgggcgagg
    20041 aatttacgtc gcgacatcag gtctccttgt gcttcgtcct cgcgccgacc gatcgcgcgg
    20101 cgaccggcgc gggcaacgag gcgcgagagc tcgagcgcga tcatggcgat cgtcatcagg
    20161 ccgatcagcg ccatccacgg catcgtgatc cagctcgctg cgctcgcgaa gccgggtgcc
    20221 cagaggcgtg cggtggtcgt gagcggaatc gacatccaca gcacgatcat cgcgatcgtg
    20281 atcgcgcggt gcgcgcgctt ggggaggtgc gccggtttga cgaggcgcca ccacaccagc
    20341 agatgaaacg agcccgtgag caggactccg aacaggtact tcagcacgcg gtgttccgag
    20401 gctagcagcc ggtagtgcgg tcgtcacgag cgaagctcga cgacggccgg catcccgccg
    20461 cgcgggcgga gcgtgacgag cggttcgggc tcgacgcggg tcgcgagtag acggaggcga
    20521 gcgtgttgca ccatcgtcgc gagcgcgagc tgggcctcga gcagcgcgaa gtgcgagccg
    20581 atgcagatgc gcggaccagc gccgaacggc aggtagtgat ggcgcgggcg cgccttcttc
    20641 gcgtcgggca gcatgcgctc gggcatgaac gcatcgggcg cggggaagta atcgccacgg
    20701 cgatggatgc cgcggatgtt gatcgcgacg atcgagccgc gtggcaggcg gtggccgccg
    20761 atatcgacgt cctcgcacgc ttcgcggccg gtcatgtaga ccggtggatg gacgcgcatc
    20821 gcctcttcga tcaccgcgag attgaacggg agcttcggca ggtcttcgac ggcgaccggg
    20881 cgccgaccga atggctcgag ctcgagcgtg agcttcgcga gcacgtcggg atggcggccg
    20941 agctcgaacc acgtccaggt cagcgcgttc gcggtggtct cgtgaccggc gagcagcagc
    21001 gtcatcacct cgtcgcggac ctgcgagtcg ctgaggccac cggcctcgtc gctcgcgagc
    21061 agcaagatcg acagcacgtc accgcgatcg gtgccgcgcg cgcggccttc gcggatcagg
    21121 cgatagacga cctcgtcgag catcttcacc gcgcggcgca tctggcgatg gcgcggcaac
    21181 ggccactcgt agccgagctg gagcggcgag cgcaggttcg cttgctgcgc gcgcatgccg
    21241 agctcgagcg cttcggcgac ctgggcggcg tcgccgcgga cgtccgcgga gaacatcgtg
    21301 cgcccggcga tcgcgagcgt catctccatc atctcgttcg cgagatcgat gcgcatgccg
    21361 tgtcgccagc gcgcgagctg cttctcggtc tcggcgacca tgatgtcgcc gtacttctcg
    21421 agccgcttcg gtgcgaatgc gggcgcgagt agcttgcgat ggcgcttgtg gtcgtcgccc
    21481 tcggcgatga gcaagccgtc gccgagcagt ggcttcagga actgcagccc ggcggacttc
    21541 ttgaacgacg ctgcctgctc ggacagcacc tggtgggcga gatcggcgtc ggtgacgatg
    21601 tagatcggga tgtgcgcgag ctggatgcgc gcgatcgggc cggtgcgtgc ggcgcggtcc
    21661 tgcagatcga ggcgatcgcg ccggaactga agcatgttgc cgatgagcgg cagccccggc
    21721 acctgcggca cggacgtcac gggcggtgaa atcgcactgg tcaacgtggc cattgtgact
    21781 aaaatgcgag cgatcgctca cattgtctac tcgcgtttcg aacgccgacc gaggagcccc
    21841 cgatgcagcg ccaaacccgg accacgccac gcaaacgccc gcgccagacg cgcagcaaag
    21901 tcactgtggg gacgattctg gaggcaacca gtcgcgttct ggtcaagcag ggcttcgatg
    21961 gcctctcgac caatgcggtc gcggctgcgg cgggagtttc gatcggctcg ctctaccagt
    22021 acttcccgaa caaggaagcg ctggttgccg cgctgatcga tcggcacatg gaagagatga
    22081 atgcggcggt gctcgccgag ctcacgcgcg tcgcgcagct gccgatcgcc gaggccgcac
    22141 gctgcgtcgt cgagctgacg attcgcgccc acgcgatcga tccggatctg catcgcgtgc
    22201 tcaccgagca agtgccgcgc atcggcaagc tcgcccggct gcgcgaggtc gacgagattt
    22261 gtcatcgcat ggtcgcgggt ctgctcgcgg cacgccggga cgagattgcg attcgcgatc
    22321 cggatctcgc ggcgttcatc ctcgtctcga cgatcgaagc agtcgtgcac cgcgcagcgc
    22381 tgctgtatcc gcatcggcta cgtgatccgc gcctggtcga cgagaccacg ctgctcgtca
    22441 cccggtatct cggcgtcgcc gagaactagg ctacagtccg cggcatgatt cgcttcgctc
    22501 tgtgcagcct cgtgctcgcg cttgccgcat gcccgaagaa cggcaccacg acgcaggtgc
    22561 agaccggtgc aggctgcccg acggcggcgg gtgtctacgt cgcttcgtat gcgtcgcagg
    22621 acaacggccg cagcgggtgg acgttgccgc tgcacgctgc gccgctccaa ggtgacgcac
    22681 ccgactacgc gagcatcgac gcagcagctg cgagcgccgc tggcgttccc gctgcgccga
    22741 ccggcgcgtt gtggttggcc accgcaggag cgcaaccgtg tcaggtgaag gtcggcggct
    22801 tctacgccgc gaagatcgaa gggccgccgg cgagcgtgtc gtacggcgtc gagctcgacg
    22861 gttgcccagc gccgaagaac ccggaagaag cgggcggcgt cctgctggtg tcgaacgaat
    22921 cgccgaccgg ctgccgcttc gaggtcccgc aaccgatcgc gggtcgcctc ggcgaaatgg
    22981 acaagaacaa gaagtggcag ccaccgacga aggaatcacc gttgccgaag ccgctcgtcg
    23041 atgtgctgcc gcagaagcag tgcgcggcgc ccgcatgcga gacgctgtgg gcattcgccc
    23101 aggtcacgat cgacggcaag ccggttgcgt ggacgggcgc gatcaactgg ctgcagatcg
    23161 gcagcgagcc cgatcagtgt gcgtggcaag ccgaacggtc gagcggattc tggatcccga
    23221 ccgacagcag cgcgacgaag gtcacggagg ggcaggagca ctcgctaccg ctgatcgcgg
    23281 tgcttgccga cggtggtggc gcgaaggtcg cgctcgcaga gggcaatggt cagtacgtga
    23341 cgtacgatct gtcgccgggt cgcgccgcgc tcggccatcg cgtcgagtgg atgctcgcac
    23401 caccacaagc gtgggacgcg atcgatcacc tcggcccgat ctgcgacgaa gatcgcgatt
    23461 agctcacatc acggtgcgcg acgtgctcgt gcgcgccgcg cgaaaaccag tcgcgcattg
    23521 cactgaacgc gtgcttcgcc gcgctgacga gtcgctcgct cgcattcggg cggcgcgcga
    23581 cgtcgtcgag caggatgcca aactgctgcc agcgcgcggg gacgatgccg tcgtatgccg
    23641 acagatagct gcatgcgttt gcgacgtcag cgatgcgacc gacgatgttg cgcttgatcg
    23701 cgctgtgcag ctgcgtcggc cgctcgatga cgtacttcca accgagcgct tcctcgggat
    23761 cggcgaacgc accgatcgca gagcattgcg gcaacgccgt gatcctgccc ggcgtgatgc
    23821 cgagcgcgag caggtcctgg acgatcagcc cggagcgcat ccgttcgcgg cgatcggcga
    23881 cgacgagatg tggcgtgtag gcgagcgcgc tctcgatcgg ggcttcgaag ccatagatcc
    23941 gagtcagcag gtttttgtac tgctcgcggg tcacgcccgc cgccatcaga tcgagccaga
    24001 acccgtcgac gtcggcgtgc agatcacgcg tctcgatgtc tagacgattc aacatcgtcg
    24061 acggtggctg catggttgtc agcgccatgc gtgcaatccg tgtgcaacag atggtgcaac
    24121 cagatcacac acttctaacg gtcaacccgg gcgctaccgc acaccgcgct gtcgagcgcg
    24181 catccgtacg cacgcgaaat ttattcgctc tcgttctcgt gcgcggtgcg agccgcttcg
    24241 agcagtcgcg gatcgacgag ctcgggcttg aggacgacct tgccgtaatg cacggctttg
    24301 atctcgggac cacgcacgac gaagtcacca gcagcctcga cgtagacgac gagatgatcc
    24361 ttctccactt tgcgcacggc gccgatctct tcgcccccgt cctcgaggaa cacgtggtcg
    24421 cccacgttga ctgcgatgtt gtgaggcatg gtccctcctc gctggcagtc tagctccgtc
    24481 acgtgatgcg agcacgcgca ttgcgagtct cggcctgtgc gcctagcacc gaaatgcgca
    24541 cggtccgtcg acgagcggca tgcagctcgc agtagcgata ttcaaagtga atcccctacg
    24601 caagctgtac aacaagttgt gccgcgagct cgcacagagc gagcgtttcg cggccacaca
    24661 tgtgcggcgc gaggcgcgac gtctcggcga cacgccgccg gcgcgtgcgc tgcgggcatt
    24721 agggggccat gcgcaagcga tgtgttcgcg gctcgacggt gtcgagcagc cgccacaacc
    24781 gatcggcgtg cttgccggcc gcaaggtcgg cgagctgttc ttggtgctgc gctacttctt
    24841 gttcgctcgg ctgatccacg tcgagcgttc gtatcgcggc acgctactcg ggtttcacga
    24901 gagcctctcg atcggtcgcc tcttgcgcga cgtggccgag cgactcgacg agcaggagat
    24961 ggtcgggttc tgcgagcaat ggctcgagca gcgcgaaccg ttgctcgcga tcgccgagcg
    25021 cgagctgcac tggttcgccg acaccccccg tcaggcgatc aggcccgggt tccggatgtc
    25081 ggcgttgcgc gagtgaccat cgccagttag ctgcgaaaaa aatctgcaac cgttaggtgg
    25141 aggctaccca ctagacagta cgaacaccgc tccgcttgcg cgtaaccgtt caggggatac
    25201 acgtgcttgc ttcttgggtg caggcggagc ggtagttcgc caacctcagt gcgcgacgta
    25261 cagtgccgcg acgagcagcg cgagcacgag gacagcgatt cccagcatgc tggtgtgacc
    25321 gctcagtgcg cgctcgcggg ccgccatgtc tcgtgtatcg gacggcgcgt cgtaccagaa
    25381 ttcgggcatg gccattggcc agatgaactg catggggtcg gccaatttct gggacccgga
    25441 agtaccagtt cgcaggctgc atcgatcggc aacatctagg acagagcgct acagcgcgcg
    25501 catccgcgca gttctctagt accgtccagg ggaatggtgg aacgcgcccc cgctgcgtcg
    25561 cgcaagatgg tgacgtcgcg cgccggctgg atgggcatgg cgcgcgacga ggaatcgcgc
    25621 gcgtatctgc aatcgcgcct cgttttgatg tcgaagctca tgttctggag cttcatcgtc
    25681 ctgctcggct cgatggttgc gttgtacgcg cagtacccga agaaggagcc ggacggcaat
    25741 cgccagatct atatcttcgc ggcgatcggc gtggtcacgc tcgccgtgat ctggcgcggc
    25801 tttctgctgc gccgtcagct caagctcgag tggctctacg cgatcgattt cttctacgcg
    25861 gccgggaccg gcagcatctt cgcgagtgcc gcgtatatcg catcgtcgct tcgcctgtcg
    25921 gcgatggtga acttgatctg ggcgatcctc atcacgttcc ttcgcacgat cgtcgtgccg
    25981 agcaccggca ggcgcacagc gatcgcgggc acgctcgtgt tcttgccact gatgctcgcc
    26041 gccgttggtc tcgcgatcac gacaccacaa gagcttccgc ccgacgcgta tgtcgcgagc
    26101 gcgctgatga tcagcgtgac tgtcgtgctg ctcgcgacga tcggctcgcg cgtgatctac
    26161 ggtctgcgcc ggcaggccga cgtggcgatg cgactcggtc agtacacgct cgataacaag
    26221 atcgccgacg gtggcaacgg aagcgtctat cgcgcgcatc acgcgctact gcgccggccg
    26281 accgcgatca agctgatgct gcccgaccgc atcggcgccg agaccgtcga tcgcttcgag
    26341 cgcgaagtgc agcacatgag tcggctcacg cactggaaca acgtgtcggt gtacgactac
    26401 ggccgtaatc ccgacggcat gttctattac gcgatggagt atctcgatgg tctcaacctc
    26461 gagaacctcg tgatcgactt cggtcgtcag cccgccgatc gcgtcatcgc gatcctccgc
    26521 caggtctgcg ccgcgctcaa cgaagcgcat cgcctcggca tcattcatcg agacatcaag
    26581 ccggcgaaca tcatcctgtg ccaccgcggc gacgtgccgg atgtcgcgaa ggtcgtcgac
    26641 tacggtctcg tcaaagagat cacggcggag aagggcgagt cgatgatcct cgggaccccg
    26701 tcgtacgtcg cgcccgaagc ggtgaccgat ccggcgaccg tcggtccggc cgcggacctg
    26761 tacgcggtcg gcctggtcgg ctacttcctg ctcaccgggc ggcgcgcgtt cgagggcaac
    26821 accacggtcg acgtctgcgt ccagcacgtg accgctcagc ccaagtcgcc gtcgagccac
    26881 ggcgtgaaga tgccggccga gctcgagcag ctcatcctgc agtgcctcgc caagcagcca
    26941 agcggtagac ctgccgacgc cggcaccctc gcgaaattgc tagcggccgt gccaccagcg
    27001 ggggactgga gcgaggacga agcgcagcgc tggtggagcg acttccgcaa gctcgaccga
    27061 ccgaccgcgg caagtgccga caccctcacg atcacggtgg atatcgagga tcgcgacgag
    27121 gccggcgcgg cctaatacgg cgagatacgt agagctctcc gccggttacg cccgggttat
    27181 cgaaggacca aatcgctagg tctgtagtcg cctgtgtgtt acgtcctacc ttatgcgtcg
    27241 aatcgggctt gttgttatgg cggcgttctt ggtgaccgcg aacatggcgg gtgcagagcc
    27301 ccggcgcggt ttgacgagtc aaccggccaa ggctgcgcgc gtcaaagcgc cccttgcgaa
    27361 gccgacgccg cccgcgcccc gcagcatcaa gccggtggcg aaggcggcga tgccggtcaa
    27421 cgagccagtc gccgagagca cgatcgacaa cccgaagccg ctgccgattc cagcgaacgc
    27481 gttgctcatg cagtaccagc gtgtcggtcg cgatctgctg ctgctcaaca acgagcggcg
    27541 tgcccgagaa ggcgcggaga acatcgacgc gaaggagtcg tgcaacgagc tgcagacgac
    27601 gtttcgctcg attaaggtcg aggacgcagt gaagacgccc gagtcgcgcg cggctgctgc
    27661 tgccgtgctc gcggagctgc acgcgaagat cgtgcgcctt cgtggtgtcg agctctcgca
    27721 agagtgcttg aagaacccgc tcgcaaaaga ctgcacttag atcgaatcga gaatcgcgcg
    27781 gcgcctctgc tgatactcgg cctcggtgat cgtgccggcc gcgcgtaatt gatcgagctg
    27841 gtgcaaacgc gccgcggtgt ctccgttggc tggcgccttc tgcacgacgg ccggatcgct
    27901 cgggagattg cagccggtcg cttcgacaag ctcgacctgt tgccagcggc gcgtcgatgc
    27961 ttgcaccgcg ggcttgggcc ccttcggcag cgagccgaac gaatatgcga gcagaccgat
    28021 gccggtgccg atcatcgcgc cgccgagcaa gtaaccaccg gtcggcgagg gcggctcctc
    28081 gtagagcggg tcacctggct cgaagatcgt gtgcgagcgc accttcgcgg cgatcatcac
    28141 cgacgcgccg agaaagccga gcacgacagc gccggcgaca tcattgacgc gtcccttcgg
    28201 cacgggctcg agctcgaacg gctgctcgta ggtgatctcg gcctgctgca cgcagtgcgt
    28261 ccgcgagatg ctatcggtgc tgcccgagag tcccgcgacc gcgatgccgt tgccttgtgc
    28321 gccggcaaag cccgcgttca gcgacgagga ttttgttgtc gcgagcgatg gctcgccgag
    28381 caagcgacgc tcgacctcgt gacgcggtcc gtagaccgta ttcgttcccc atttcgtaca
    28441 gcccgcgaat gtcgtgacag ccagcaaaca agaaagtgtg cgcatggttt gcgagccaac
    28501 gcatgggaac agcattcgcg tccctgcgtg gtgttgcgca gtgtgaagtg attggccgaa
    28561 ccaatcatgt tcgtaacgag tttgttcgcg aggtcgactc gcacagtggt atgggttgcg
    28621 gccgccatga cgaacgacga tcggaaacgc agcgaacaac tcgcggcgca ggacacgctc
    28681 gccggttacg accggtgggc tgcgtcgtac gacgccgagc cgaacgcgct gatgtcggcg
    28741 accgcatggg tgctcgaccg cgcgccactc ggctgcgcag actgcgacgt actcgagctc
    28801 ggctgtggca ccggccgcaa cgcgcagcgc gtgatcgccg agggcgcccg tagctacacc
    28861 ggtctcgatg gctcgcaagg catgctcgcg gtcgcggcgc agagatacaa cgatccgcgc
    28921 atcagcttca cggct
//
LOCUS       DASUFX010000603         5098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1070317, whole genome shotgun sequence.
ACCESSION   DASUFX010000603 DASUFX010000000
VERSION     DASUFX010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070317"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFV99_24265"
     CDS             complement(<1..694)
                     /locus_tag="VFV99_24265"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5062518.1"
                     /translation="MALKKGAGVTPAEGKLGILLPGMGAVATTFIAGVQAIRRGLGKP
                     IGSLTQLGHIRIGKRTDGNSPAIKDYVSLAKLDDIVFGGWDIFPDNAYQAAVRAGVLD
                     TRLLDQLKPELEAIKPMTAVFEQAFVKKLNGPNLKKGTSKMDLANQVMDDIKTFKATN
                     KCDRLVAVWCGSTEVYRKPSDVHSSLAKFEAGLERSDDDIAPSQIYAYACLKMGVPYA
                     NGAPNLSVDTPAL"
     gene            complement(791..1546)
                     /locus_tag="VFV99_24270"
     CDS             complement(791..1546)
                     /locus_tag="VFV99_24270"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5062519.1"
                     /translation="MHPRRLYAHRGASAERPENTLVAFQRAMEIGVDSLELDVHLTRD
                     RVFIVHHDDTALRMCGSPLKWSDIDLVEAQRLDAGWGFVAKDGSRPFAGKGITVPTFE
                     SILREFPRVHINVDLKGEDAVVALLELVQRLKFEERVTIASFHTATMVSVRRHGYAGE
                     TGLSQGEVASLLAMPAVLWRQLPFTGTAAQVPTKQGPVRFDRPTFIAKCHNLGLRVDF
                     WTIDDPAEAKRLLALGADGIITNDPAALKPLFS"
     gene            complement(1546..2028)
                     /locus_tag="VFV99_24275"
     CDS             complement(1546..2028)
                     /locus_tag="VFV99_24275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5062520.1"
                     /translation="MKIEKGRRVTLKVDISVAGGQTLEKKTLEFVQGSGPMLPALERI
                     LEGLEAGAKRDGVLKAKDAFGSPEVQAKIPLKKMKRSEFPKDATLKVGDRMVAAGADA
                     AHMNVILEIAKVSGDDVEVKLIHPLADKDLKYSIEVVAVRDTRPPPVPAAALDLEEEK
                     "
     gene            2184..2564
                     /locus_tag="VFV99_24280"
     CDS             2184..2564
                     /locus_tag="VFV99_24280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062521.1"
                     /translation="MLRLVVIVSLALAAATGCHSGKSPELRVLGVHDAPQSHVFVQVT
                     NPARRPMKLTKLEYVFASASSGATLAEGEMQLYREIPAGAAAVVEVPLDAEANESLML
                     RGTLTAELDQVVRSFKLKAQIQPH"
     gene            2621..3373
                     /locus_tag="VFV99_24285"
     CDS             2621..3373
                     /locus_tag="VFV99_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062522.1"
                     /translation="MKHLWMILVLACVFVTPASAKKYGRQVRYAGIHPVSKAEGGGIC
                     HIEFPHVHVYPANKLEYRVVADNNVFVGDPVAYGWDGPKYAYKGAHPIQVNVLAGTPE
                     PYVHYCYLSGPHYHYFEPADDDDYKVVGDAYFYVGTPPPAMIEARPAYVGINAVYTPL
                     VYTRPVIEVEPPSAWIMLQPGFVVEAAAPVVVAPAPPRGHIVGGVGVSAGVSVHIPVP
                     TVSVGVQVGGPAVIVPAGRGHGKFKHKKWKRR"
     gene            3383..3757
                     /locus_tag="VFV99_24290"
     CDS             3383..3757
                     /locus_tag="VFV99_24290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062523.1"
                     /translation="MRAWLLIALALAGAACSKGKGASSRDGVVDAWKKGGLETSPLTA
                     AKSSIGNDCATGTVSKLDVMVCTFKTAKEAKDAEDKGLEWVGDNTGASKAQGEMLIVV
                     ADRKKVDPQGKVINQVITLPAN"
     gene            complement(3758..4036)
                     /locus_tag="VFV99_24295"
     CDS             complement(3758..4036)
                     /locus_tag="VFV99_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062524.1"
                     /translation="MWRACVGLFVAACSFDRIVSRDMWYALRLRIIDNAITFFVDGQQ
                     IGTASKTSDGNFAGLFAYNCDVDFDDVRVRMAVNPAPTATIGAQQDCN"
     gene            complement(4075..>5098)
                     /locus_tag="VFV99_24300"
     CDS             complement(4075..>5098)
                     /locus_tag="VFV99_24300"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5062525.1"
                     /translation="TSAPAIANDRVFASYPAAQSAEDEGKPRPPGATHALAAFELKTG
                     KILWQLWLESDVMSAPVAVGEFVYVTTFTGTVIKIEQATGKVRYAMKAQVTSAPVVQF
                     SDDGLESMYYTSGEYWKDELGAYRKQESVIRADHNEPQTKYKAATKKADYIDAKVQGS
                     TAQNKAAKEDDAANGFSGGAPASAAADYAFESVGVESVSSMQSFQGSRVLRFADKSVN
                     TMGDEVIATDADSGKTLWSYKLDGDTKKQGGFLGTAPLAAGKSVLFATLGGSVIQLDP
                     GSGKRKAEFKVGSPVRSQPVVDEGWIYVGTEDGKLVAIDTKDKTVHGWPTWGGNAQRS
                     GIAPKL"
BASE COUNT          847 a         1626 c         1659 g          966 t
ORIGIN      
        1 ggagcgcggg cgtgtcgacc gacaggttcg gtgcgccgtt cgcgtacggc acgcccatct
       61 tgaggcacgc gtacgcgtag atctgcgacg gcgcgatgtc gtcatcggaa cgctcgaggc
      121 cagcctcgaa cttcgcgagc gagctgtgga cgtcgcttgg cttgcgatag acctcggtcg
      181 agccgcacca gaccgcgacg aggcgatcgc acttgttggt cgccttgaac gtcttgatgt
      241 cgtccatcac ctggttggcg aggtccatct tcgacgtgcc cttcttcaga ttcgggccgt
      301 tgagcttctt cacgaacgct tgctcgaaca ccgcggtcat cggcttgatc gcttcgagct
      361 cgggcttgag ctgatcgagg agccgcgtgt cgagcacgcc ggcgcgaacc gccgcttgat
      421 acgcgttgtc ggggaagatg tcccaaccgc cgaacacgat gtcgtcgagc ttcgcgagcg
      481 agacgtagtc cttgatcgcg ggcgagttgc catcggtgcg cttgccgatg cggatgtgac
      541 cgagctgagt cagcgagccg atcggtttgc caagaccgcg gcggatcgcc tgcacacctg
      601 cgatgaacgt cgtcgcaacc gcgcccatac cgggcagaag gattccaagc ttgccttcgg
      661 cgggcgttac gccagcgcct ttcttgagag ccatgcgagc ccgtgtacca cgattgcggc
      721 tcagcgagac ttcgagggcg gtggtttgcc gggcttggcc agaggctgtc ggttcgccga
      781 cggtcgtcgg ttagctgaac agcggcttca gtgcggccgg atcgttcgtg atgatgccgt
      841 cggcgccgag ggcgagcagg cgcttggcct cggcggggtc gtcgatcgtc cagaaatcga
      901 cgcgcagacc gagattgtgg cacttcgcga tgaacgtcgg gcgatcgaag cggaccggtc
      961 cctgcttcgt cggcacctgc gctgcggtgc cggtgaacgg tagctggcgc cacagcacag
     1021 cgggcatcgc gagcagcgac gcgacttcgc cctgcgagag gccggtctcg ccggcgtagc
     1081 cgtggcgtcg taccgagacc atcgtcgcgg tgtgaaagct cgcgatcgtc acgcgctctt
     1141 cgaacttcag ccgctgcacg agctcgagca gcgcgaccac cgcgtcttcg cctttgagat
     1201 cgacattgat gtgtacgcgt gggaactcgc gaaggatcga ttcgaacgtc ggcacggtga
     1261 tgcctttgcc ggcgaacggc cgcgagccgt ctttcgcgac gaagccccaa cccgcatcga
     1321 gtcgctgcgc ctcgacgaga tcgatgtcgc tccacttcag cggcgagcca cacatgcgca
     1381 gcgcggtgtc gtcgtgatgc acgatgaaca cgcgatcgcg cgtgagatgg acgtcgagct
     1441 cgagcgagtc gacgccgatc tccatcgcac gctggaatgc gacgagcgtg ttctccgggc
     1501 gctcggccga ggcaccgcga tgtgcgtaca gccgacgggg atgcactact tctcttcttc
     1561 gagatcgagt gccgctgcgg gcaccggcgg cggacgggta tcgcgaaccg cgacgacttc
     1621 gatcgagtac ttgagatcct tgtccgcgag cggatggatc aacttcacct cgacgtcatc
     1681 gccgctgacc ttggcgatct cgaggatcac gttcatgtgc gcggcatcgg cgccggcggc
     1741 aaccatgcga tcgccgacct tgagcgtggc gtccttcggg aactcgctgc gcttcatctt
     1801 cttgagcggg attttggcct ggacttcagg gctgccgaag gcgtccttcg ccttcagcac
     1861 gccgtcgcgc ttggcgcccg cctcgaggcc ttcgaggatg cgctcgagcg ccggcagcat
     1921 cgggccggag ccctggacga attcgagggt cttcttctcg agtgtctgcc ccccggccac
     1981 cgagatgtcc actttgaggg ttacgcgtcg acctttctcg atcttcacgc tgaaacgtta
     2041 acgcgatcac gtagggatcc gcgacagggc ggccacgctt ctgtgaacgg ggaatcgcga
     2101 tccgtggtca gttgatcgtg accacgcgga cttgcgttta tttggacgcc tggcatgaaa
     2161 gccgcacaat agttttcccg ctaatgcttc gcttggttgt aattgtctcg ctcgcgctcg
     2221 ccgctgccac tggttgccac agtggcaagt cgcccgagct tcgcgtcctt ggtgtgcacg
     2281 acgcaccgca aagccacgtg ttcgtgcagg tcacaaatcc cgcgcgtcgc ccgatgaagc
     2341 tgacgaagct cgagtacgtg ttcgcgtcgg catccagcgg cgccacgctc gcggaaggcg
     2401 agatgcagct ctatcgcgag attcccgccg gtgcagccgc ggttgtggaa gtgccgctcg
     2461 acgccgaagc gaacgagtcg ctgatgcttc gcggaactct tacggcggag ctcgatcagg
     2521 ttgtgcgcag cttcaagctg aaggcccaga tccaaccaca ctgaaccttg attctccggc
     2581 gggagcgcgc ttcccttaga gtgccgtcga gggaggcacc atgaagcacc tctggatgat
     2641 tctcgttctc gcctgcgtat ttgtgacgcc ggcgtcggcc aagaagtacg gtcgccaggt
     2701 ccggtatgcc ggcattcatc cggtgtccaa ggccgagggt ggcggcatct gccatatcga
     2761 gttcccgcac gtccacgtgt acccggcgaa caagctcgag taccgggtgg tcgccgacaa
     2821 caacgtgttc gttggcgacc cggtcgcata cggctgggac ggccccaagt acgcatacaa
     2881 gggcgcgcac ccgatccagg tcaacgtgct ggccggtacg cccgagccgt acgtgcatta
     2941 ctgctacctg agcggcccgc actaccacta cttcgagccg gccgatgacg acgactacaa
     3001 ggttgtcggc gacgcgtact tctacgtcgg cacaccgccg ccagcgatga tcgaagcgcg
     3061 cccggcgtac gtcggcatca acgcggtcta cacgccgctc gtctatacgc gccccgtcat
     3121 cgaggtcgag ccgccgagcg cgtggatcat gttgcagccc ggcttcgtcg tcgaagctgc
     3181 cgcacccgtc gtggtcgctc cggctccgcc gcgtggccac atcgtcggtg gcgttggcgt
     3241 gtcggccggc gtgtcggtgc acattccggt gccgacggtt tcggttggtg ttcaagtcgg
     3301 tggcccggcg gtgatcgttc cggcggggcg cggccacggc aagttcaagc acaagaagtg
     3361 gaagcgccgc taagaggaac ccatgagagc ttggcttttg atcgcgctcg cgctcgcagg
     3421 tgcggcgtgc agcaaaggga aaggcgcgtc gtcgcgtgac ggcgtcgtcg atgcatggaa
     3481 gaagggcggc ctcgagacgt caccgctcac tgccgcaaag tcgagcatcg ggaatgactg
     3541 tgcgaccggt acggtgagca agctcgacgt catggtctgc acgttcaaga ccgcgaagga
     3601 agcgaaggac gccgaggaca agggcctcga gtgggtcggt gacaataccg gcgcgtcgaa
     3661 ggcgcagggc gagatgctga tcgtcgtcgc cgatcgcaag aaggtcgatc cgcagggcaa
     3721 ggtgatcaat caagtgatca ccttgccggc gaactgatca gttgcagtcc tgctgcgcgc
     3781 cgatcgtcgc ggtcggcgcc ggattgacgg ccattctcac gcgcacgtcg tcgaagtcga
     3841 cgtcacagtt gtaggcgaac aggccggcga agttgccatc cgaggtcttc gacgctgtcc
     3901 cgatctgttg gccgtcgacg aagaacgtga tcgcgttgtc gatgatacgc agccgcagcg
     3961 cgtaccacat gtcacgcgac acgatgcgat cgaaagagca tgcggcaacg aacagaccga
     4021 cacacgctcg ccacacgcca tcacggtatc acgcgctatc gcgcggcagg ctcgctagag
     4081 cttcggtgcg atgcccgagc gctgtgcgtt accgccccac gtcggccacc cgtgcacggt
     4141 cttgtccttg gtgtcgatcg cgacgagctt gccgtcttcg gtgccgacgt agatccagcc
     4201 ttcgtcgacg acaggttgcg agcgcaccgg cgagccgacc ttgaactcgg ccttgcgctt
     4261 gccgctaccc gggtcgagct ggatcacgga gccgccgagc gtggcgaaca gcaccgactt
     4321 gccggccgcg agcggcgcgg tgccgagaaa gccgccttgc ttcttcgtat cgccatcgag
     4381 cttgtacgac cacagcgttt tgccgctgtc ggcatcggtc gcgatgacct catcacccat
     4441 cgtgttcaca ctcttgtcgg cgaagcgcag cacgcgcgag ccctggaacg attgcatcga
     4501 cgacacgctc tcgacgccga cgctctcgaa tgcgtaatcc gccgcggctg atgcgggcgc
     4561 gccgccggag aagccgttcg ccgcatcgtc ttccttcgcc gccttgttct gtgcggtgct
     4621 cccttggacc ttcgcgtcga tgtagtcggc cttcttcgtc gcggccttgt acttggtctg
     4681 cggctcgttg tgatcggcgc ggatcaccga ctcctgcttg cgatacgcgc cgagctcgtc
     4741 tttccagtac tcgccgctcg tgtagtacat cgactcgaga ccgtcgtcgg aaaactgcac
     4801 gacgggcgcc gacgtgacct gcgccttcat cgcgtagcgc accttgccgg tcgcctgctc
     4861 gatcttgatc acggtcccgg tgaacgtggt gacgtagacg aactcgccga cggcgacggg
     4921 cgccgacatg acgtcgctct cgagccacag ctgccacaag atcttgccgg tcttgagctc
     4981 gaacgcggcg agcgcgtgcg tggccccggg cgggcgcggc ttgccctcgt cctcggccga
     5041 ctgggcggcc gggtacgacg cgaacacgcg atcgttcgcg atcgccggcg cgctggtc
//
LOCUS       DASUFX010000604         5574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1209476, whole genome shotgun sequence.
ACCESSION   DASUFX010000604 DASUFX010000000
VERSION     DASUFX010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209476"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFV99_24305"
     CDS             complement(<1..919)
                     /locus_tag="VFV99_24305"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5062526.1"
                     /translation="MRSALSLILLAAACGSGKPTSTQPMPPPPSGDRAPAPDKPAEPV
                     ADQGPQKPIKNATLASIGLDPEAIDRKADPCDDFYQFACGNWIAKTEIPADKPATMRS
                     FVAIQDRNDDYLHDMLEKARTSPGTDPVMQKLGAFYGSCMDEAAIAKAGIKPLQPLLA
                     AINKVKDAKSLSSAVATLEAAGVGTLWQFGPIEDSADATRTIAGIDQGGIGVPDRDYY
                     LVDEPQNKAVRDAYAQVVTTMLVAAGHKADAAKQETADILALETEIAKVSKDKVARRD
                     PKGTYNKIDRAGVAKAMPHFDWETYWKTRG"
     gene            1053..2159
                     /locus_tag="VFV99_24310"
     CDS             1053..2159
                     /locus_tag="VFV99_24310"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX5062527.1"
                     /translation="MRHAIGLSAVIAISSVALANPESAKAPPQAAPQAAGPQQQAAPP
                     QPAKSPGSLSETAKLLEPIQVESLTLTPIVTTKQKVDDEKLLVLDEAMPKKLVRIHEM
                     DDEDVNNLTLTNKSEQPLFLLAGEVIIGGKQDRIIGRNTIVPPKKTLSVPVFCVEHGR
                     WDNKTKEFTTAKALAHGRLRGSANFDTQSDVWKEVAAKNELRKTTSSTDTYRKVATQQ
                     SDGTLAKWEKQVDTALGKLGDADKAKLVGFAVSLNGKVATVDVFDNPTLFKKLQGKLV
                     RSYITEAIDVKKEKNIKAPTTKDVTDFIADAEKAQEEDAYSTDLADTRTKKGSKSAKA
                     RVMYKASKSAGAGAADAAPAAAEGKAVFESYQAR"
     gene            2634..3077
                     /locus_tag="VFV99_24315"
     CDS             2634..3077
                     /locus_tag="VFV99_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062528.1"
                     /translation="MGWQHVADDDAEPFTQRVPCLSVGVGGWMAPAAAITLRLSAISS
                     DPPHSSGLVVQALLGPALQLWPSDSIWLGAGVGLAFLRKTNDTDTAASVGFEARAGYT
                     FNRHGKHSINASIEMLTARFTNDDSSLHFTGRMTSVGVLLGYQLL"
     gene            3384..4706
                     /locus_tag="VFV99_24320"
     CDS             3384..4706
                     /locus_tag="VFV99_24320"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5062529.1"
                     /translation="MDSSRAAAEPLPGVVWQNRVLAHPQNASPLTSVSHILYLNDCKN
                     NSCRVSPGADNSLTNRSSIAQSTVTLPAYPYSDAHWSSLVACVRETFAPFDIAITTTD
                     PGTMTSHFEVMIGGSSGNLSPGSQWGGVAPYIGCGATENNVISFVFPQTSSDLDYLCA
                     VVAQEASHVWGLDHELNAKDPMTYLDLLASKRFQDEASSCGESLGAPRYCNCGGSTQN
                     SKRYLLDTFGPSTPPSLAITSPLEGQWLKPGFTVTAELTSSVGGKAGELAVDGVSVES
                     TTTSPFMFTAPSDLIGGSHAVTVSATDLANRAAMATVNVNVMAHCNAVEPCTSGSCLG
                     GYCVPDASVSGGLGATCTAADQCITGLCVTNGGESLCTGACDAGDTCPAGFECIATAF
                     GAACWPDDDDHGGSGGGGGGCNASGGASWMSLAFAAGVFVLARRRRRS"
     gene            4868..>5574
                     /locus_tag="VFV99_24325"
     CDS             4868..>5574
                     /locus_tag="VFV99_24325"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5062530.1"
                     /translation="MRHLALLAVFLPAVALAQPGPPPPPPPPPGTPAPAPTADPAAPA
                     PAPAPAPAPAPAPAPPPAGGYYQPVQPVYGPPPQPVSLKNGMTFEANLGLGWMTSSEA
                     DDTSDLGIGGLSLGVGGWVSPQIAITGRLAGVTLSENSATLSNIFLGPSLQYWVNDNF
                     WLGGGIGLGILAADCDGCQSDSITGFGFDMRIGYTFSVTSENTFNASFELNPEHYSEN
                     GGSATITGIGILLGYQHL"
BASE COUNT          990 a         1807 c         1774 g         1003 t
ORIGIN      
        1 ggccgcgcgt cttccagtac gtctcccaat cgaagtgcgg catcgccttc gcaacgcccg
       61 cgcgatcgat cttgttgtac gtgcccttcg gatcgcggcg cgcgaccttg tccttcgaca
      121 ccttcgcgat ctcggtctcg agcgcgagga tgtcggcggt ctcctgcttc gcggcgtcgg
      181 ccttgtgacc ggcggcgacg agcatcgtcg tgacgacttg cgcgtaggca tcgcgtactg
      241 ctttgttctg cggctcgtcg acgaggtagt agtcgcgatc ggggacgccg atgccgccct
      301 gatcgatgcc ggcgatcgtg cgcgtcgcat cggccgaatc ctcgatcggg ccgaactgcc
      361 acagcgtgcc gacgccggcc gcttcgagcg tcgcgaccgc gctcgacagc gacttcgcat
      421 ccttcacctt gttgatcgcg gcgagtagcg gctgcagcgg cttgatgccg gccttcgcga
      481 tcgccgcctc gtccatgcac gagccgtaga acgcgccgag cttctgcatc accggatcgg
      541 tgcccgggct cgtgcgcgct ttctcgagca tgtcgtgcaa gtagtcatca ttgcgatcct
      601 ggatcgcgac gaagctgcgc atcgtggccg gcttgtccgc cgggatctcg gtcttcgcga
      661 tccagttgcc acacgcgaac tgatagaagt cgtcgcaagg atcggctttg cgatcgatcg
      721 cttcgggatc gagaccgatc gatgcgagcg tcgcattctt gatcggcttc tgcggaccct
      781 gatcggccac cggctcggca ggtttgtcgg gcgccggcgc cctgtccccg ctcggcggcg
      841 gtggcatcgg ctgtgtcgag gttggtttcc cggagccgca ggcggcggcg agcagaatca
      901 gcgagagtgc actgcgcatg attgtcgtta tcacgcgtgg tttctgcgat ctacgcgtgt
      961 cgcagcggtc gcacggcgtc gcacgccgag aaattttcgc aacggcctca gatcgaccgt
     1021 gtcgatggcg cgttccagcc aaaggaggct tcatgcgtca cgccataggt ctgtccgccg
     1081 tcatcgcgat ctcgagcgtc gcgctggcga atcccgagtc cgcaaaagcg ccaccgcaag
     1141 cagcaccgca agcagcaggg ccgcaacagc aggcggcacc gccacaaccg gccaagagtc
     1201 cgggctcgct gtccgagacc gcgaagctgc tcgagccgat tcaggtcgag agcctgacgc
     1261 tgacgccgat cgtcacgacg aagcagaagg tcgacgacga aaagctgctg gtcctcgacg
     1321 aggcgatgcc gaagaagctc gtgcgcatcc acgaaatgga tgacgaggac gtcaacaacc
     1381 tcacgctcac gaacaaatcc gagcagccgc tgttcctgct cgccggcgaa gtgatcatcg
     1441 gcggcaagca agaccgcatc atcggccgca atacgatcgt gccgccgaag aagacgttga
     1501 gcgtgccggt gttctgcgtc gagcacggcc gctgggacaa caagacgaag gagttcacga
     1561 ccgccaaggc actcgcgcac ggccggctgc gcggtagcgc gaacttcgat acccagagcg
     1621 acgtctggaa ggaagtcgcc gcgaagaacg agctccgtaa gacgacatcg tcgaccgaca
     1681 cctatcgcaa ggtcgcgacg cagcagtcgg acggcacgct cgcgaagtgg gagaagcaag
     1741 tcgacaccgc gctcggcaag ctcggcgatg ccgacaaggc gaagctcgtc ggcttcgcgg
     1801 tgtccctgaa cggcaaggtc gcgaccgtcg acgtgttcga taacccgacg ctgttcaaga
     1861 agctgcaggg caagctcgtg cgcagctaca tcaccgaagc gatcgatgtg aagaaagaaa
     1921 agaacatcaa ggcgccgacc acgaaagacg tcaccgactt catcgccgac gccgagaagg
     1981 cgcaagaaga ggacgcgtac tcgaccgacc tcgccgacac gcgcacgaag aaaggcagca
     2041 agtcggcgaa ggcgcgcgtc atgtacaagg cctcgaagag cgcaggtgct ggggccgcgg
     2101 acgcagcgcc cgcagccgcc gagggcaagg cggtcttcga gtcatatcaa gcgcgttgat
     2161 cgcgaggcgc agccgtttgt tgcggtggta caccggtgcc gtgcgcacgc gcacggttca
     2221 tcgatcgcga gacttcgaag acccacagcg gcacgtgata gcgtcgatgc gtcggaggtc
     2281 gcatgcgctg taccgcgttc ctcgtgttgc tgttgcctgc cgtcgcgttc gctcagtcgt
     2341 caggtccatc ggatccggcg tcgaatccct ccgatccgac gccagacccg acggtatcga
     2401 ctgcaccggg cgccaccgca ccaaacccga acccgccgca acccgtcgac gacgcgagca
     2461 cgatgcttcc gaatccggcg gatccgggca gtgcacccga tggctatcgc cgattgcagc
     2521 cctcgcccac gtatccgcca ccgcgtggcc ataacctgcc gacagcgacg gacgagcaat
     2581 acacaccgcc ggagcctcca cataaaggca ggacgttcga agcgggcgtt ggcatgggtt
     2641 ggcagcatgt cgcggacgac gacgccgagc cgttcacgca gcgtgttcct tgcctcagcg
     2701 ttggtgttgg cggatggatg gctcctgcgg ccgcgatcac cctgcggttg tcagccatct
     2761 cctccgatcc gccgcatagc agtgggcttg tcgtgcaggc tcttctcgga cccgcgcttc
     2821 agttatggcc ttcagattcg atttggttgg gtgccggcgt gggccttgct ttcttacgaa
     2881 agacaaacga caccgacacg gcggcgagcg tcggcttcga ggcgcgcgcg ggttacacgt
     2941 tcaatcgcca cggcaagcat tcgatcaacg catcgatcga gatgctcacc gcgcggttca
     3001 cgaacgacga ctcgtccctc catttcacgg ggcggatgac aagcgtcggc gtgctgctcg
     3061 gctaccaact tctatagcga gcgcgcgtgt tcatcgagca agtgttgctc gtgttgttac
     3121 agcgacgcga cgcgatcgct gaacaatgat tctgtaacaa tcaattgaat atcgatacgt
     3181 tgtggagcaa tgcctcgcaa cagtgacagt tttgcgcagt cgattgttac cttcacgact
     3241 tgtgcaattt gctcgcgctc gacggtaacg cgcgtgtgga agctcacgat cgcgcgctgg
     3301 catgcgtcgt gatgatgcgc tcgcgcatga caaatcgagg cgtcgtcgtc gggatgttgg
     3361 tggtgagcac ttcgctcgtg gctgtggact cttcacgcgc ggctgcggag ccgttgccgg
     3421 gcgtcgtctg gcagaaccgc gtgctcgcgc atccgcaaaa cgcatctccg ttaacgtcgg
     3481 tgtcacatat tctttatctg aatgactgca agaacaacag ttgtcgagtc agtccgggag
     3541 ccgacaattc tctgaccaat cgttcgagca tcgcgcagag cacggtcaca ctgccggcgt
     3601 atccgtacag cgacgcgcac tggagctcgc tcgtcgcgtg cgtgcgcgag acgttcgcgc
     3661 cgttcgacat cgccatcacg acaaccgacc ccggcacgat gacgtcgcac ttcgaggtca
     3721 tgatcggtgg cagctccggc aacctgagcc cgggctcgca gtggggcggc gtcgcgccgt
     3781 acatcggttg cggcgcgacc gagaacaacg tcatcagctt cgttttcccc cagacgtcga
     3841 gtgacctcga ttatctgtgc gcggtcgtcg cacaggaggc ctcgcacgtc tgggggcttg
     3901 accacgagct caacgcgaaa gatccgatga cgtatctcga tctgctcgcc tcgaaacgct
     3961 tccaggacga ggcgtcgtcg tgcggcgaga gtctcggcgc gcctcgctac tgcaactgcg
     4021 gtggctcgac gcagaacagc aagcgctacc tgctcgacac gttcggccca tcgacgccgc
     4081 cgtcgctcgc gatcacgtcg ccactcgaag gtcagtggct aaagcccggg ttcacggtca
     4141 cggccgagct gacatcgagc gtcggcggca aagccggcga gctcgcggtc gacggcgtga
     4201 gcgtcgagtc gacgacgacc tcgccgttca tgttcaccgc gccgagcgac ctcatcggcg
     4261 gttctcacgc tgtcacggtc tcagcgacgg atctcgcgaa ccgcgccgcg atggccacgg
     4321 tgaacgtcaa cgtcatggcg cactgtaatg ccgtcgagcc gtgcaccagt gggtcgtgcc
     4381 tcggcggcta ctgcgtaccc gacgcgagcg tttccggtgg gctcggcgcg acgtgcacgg
     4441 ccgctgatca atgcatcacc ggtctgtgcg tgacgaacgg cggcgagtca ctgtgcacgg
     4501 gcgcgtgcga cgcgggcgac acgtgcccgg ctggcttcga gtgcatcgcg accgcgttcg
     4561 gcgccgcgtg ctggcccgac gatgacgatc atggaggcag tggtggtgga ggaggtggct
     4621 gcaacgcgag cggcggcgca tcatggatgt cgctcgcgtt tgcagcgggg gtgttcgtgc
     4681 tcgcgcgtcg ccgccgtagg tcttgatcga gcgtcgctgc taatcgatcg ctgccgcgaa
     4741 tcgttgcagc acacgcgtca cccatgtggg tcctggcgcg tcacgccgcg tggcaaaagt
     4801 ctgccgatct cttctctcgc cacattttgc ctggacactg cgatcggctg tgactagcgt
     4861 gggccggatg cgtcacctcg ctttgcttgc cgtgttcctt cccgcagtcg cactcgcgca
     4921 acctggtccg ccgccgccac cgccgccgcc gcccggcacg ccggccccgg cccccacggc
     4981 tgatccggcc gctccggctc ctgcgccagc acctgctccg gcacccgcgc cggcacctgc
     5041 gccgcctccg gcgggcggtt actaccaacc ggtgcagccg gtgtacggac cgccgccgca
     5101 accggtgagc ttgaaaaacg gcatgacgtt cgaggcgaac ctcggccttg gctggatgac
     5161 gtcatcggag gccgacgaca cgtcggacct cggcatcggt ggtttgagcc ttggcgtcgg
     5221 tggttgggtt agcccgcaga tcgcgattac cgggcgtctt gccggcgtga cgctcagcga
     5281 gaacagcgcg acgctcagca acatcttcct tggcccgtcg ctccagtact gggtcaacga
     5341 caacttctgg ctcggcggtg gcatcggact cggcatcctc gcggccgact gcgacggctg
     5401 tcagagtgac agcatcaccg gcttcggctt cgacatgcgc attggctaca cgttctcggt
     5461 caccagcgag aacacgttca atgcgtcgtt cgagctaaac ccggagcatt acagcgaaaa
     5521 cggtggctcg gcgacgatca cgggtatcgg gattctgctc gggtaccagc acct
//
LOCUS       DASUFX010000605         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2043346, whole genome shotgun sequence.
ACCESSION   DASUFX010000605 DASUFX010000000
VERSION     DASUFX010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043346"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFV99_24330"
     CDS             complement(<1..426)
                     /locus_tag="VFV99_24330"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5062531.1"
                     /translation="MTEKFLARHTTASLEQLTGGKKGVIALVPIGSTEPHGPHLGLGT
                     DVIISAAACLRACELLEKRGTMVGVIAPAVSYGVTECATGFAGAVSIPADVLTAYVGA
                     ICDGLLASGVRHVCLVNNHLEPAHDAAIRAVLTGRENKVS"
     gene            complement(423..2066)
                     /locus_tag="VFV99_24335"
     CDS             complement(423..2066)
                     /locus_tag="VFV99_24335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX5062532.1"
                     /translation="MSDVRFPDDFNLADYWLFDRLKEGKGNRVALRFGERSYTYADVA
                     ERSRALARSLIKAGLKPEQRVYIVLPDTPPFVWSIFGTLAAGGVVTMGNPQSPIEDLA
                     YVIEYVRAAFVITTPTIVKALAQVMPKERRAGAIIMDSPDAATGDDPEPLPGEPGTLE
                     YAIELGKNSDIPLPKIHRDDQAIWLFTSGSTGKPKANMHTHRDFAFNTEVFAKRTIGM
                     REDDVTVSVPRLFFGYATGTNLWFPFAVGATVGLFSERPTAESITAAIQRYKATVVTN
                     VPTMLGKILDLDDERRARGEPGLDLSTVRFHFSAGEALPEPLMRRFVERFGGEIYDGI
                     GSAEMFHIYCSNRPGDVKPGTLGRAVEGYELKILAEDADGPGAPELPRGDIGVLWVKG
                     DSVSQGYFRDREKSWKTFHGHWCRTGDLFSIDEAGYLYFQGRADDLFKVGGVFVAPRE
                     VEDCLLAHPAVSVVAVIPGEDQGLTKPKAIVVLRPDARERDKRALADELKAHVQARLS
                     KHKYPRWVLFVDDLPKNDRGKVDKKLLIEREKRGELASE"
     gene            complement(2063..3208)
                     /locus_tag="VFV99_24340"
     CDS             complement(2063..3208)
                     /locus_tag="VFV99_24340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5062533.1"
                     /translation="MRLSTDDLPMFFDATHVALADRLRAAAASFAEAELAADEPARDR
                     AAVAAFAKSGLFELVAPASGKTDARSLCLTREMLGYHSARADSIFAVQGLGVHSILLA
                     GSPEQRAQVDAFARGQQVAAFAVTEPEAGSDVASIQTHAAPRPGGYELNGDKLFISNL
                     GIADHATVFASTDQSRGAQGLSAFWVPLDTKGVTVMPLVATAPHPIGALGLRNVFVPE
                     SARIGAEGQGMKLAMQTLDAFRVSVGAAAVGMARRAMDEAIAFVTKRIQFGKPLAEQQ
                     LVQAHIADMAVDLDTARLLVLRAAHRKDTTAERVTTEVSIAKLGATEAAQRVIDRAVQ
                     LFGGRGVMAGHIVEHLYRAIRPLRIYEGTSEIQRTIIGRALAKELAR"
     gene            complement(3211..>4017)
                     /locus_tag="VFV99_24345"
     CDS             complement(3211..>4017)
                     /locus_tag="VFV99_24345"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX5062534.1"
                     /translation="GLPTAQTFSLELAAGVATITLDRPDRLNALTFEIYRELAEVFEQ
                     LDRTDEVRAVVITGKGKGFCSGGDQDDIIKHLLGRDTPGLVSFTRATGRLIAAMRKCR
                     RPIVGAINGIAVGAGAVIACACDMRIVAATAKFGFIFPKVGLCGADMGATYLLPRIVG
                     LGHASELLFFGDVIDAEHAAKIGLANRIVEDGDKALALAQEWAAKLAAGPAFAHAMTK
                     QMLESEHTMPLAAAIEAEAQAQALCMQHPDFGEAHAAFKEERPPHFVGAK"
BASE COUNT          646 a         1351 c         1347 g          673 t
ORIGIN      
        1 gctgaccttg ttctcgcggc cggtgagcac ggcgcggatg gcggcgtcgt gcgcgggctc
       61 gagatgattg ttgacgaggc acacgtggcg cacgcccgac gcgagcaggc cgtcgcagat
      121 cgcaccgacg tatgcggtca ggacgtcggc agggatcgag accgcgcccg cgaaacccgt
      181 cgcacactcg gtgacgccgt agctgacggc cggcgcgatc acgccgacca tcgtgccgcg
      241 cttttcgagg agctcgcacg cgcgcaggca ggcggcggcc gagatgatga cgtcggtacc
      301 gagcccgaga tgcggaccat gcggctccgt ggagccgatc ggcacgagcg cgatcacgcc
      361 cttcttgccg ccggtgagct gttcgagcga tgcggtcgtg tggcgggcca ggaacttctc
      421 ggtcactcgc ttgctagctc tccgcgcttt tcacgctcga tgaggagttt cttgtcgacc
      481 ttgccgcgat cgttcttcgg caggtcgtcg acgaacagga cccaacgcgg gtacttgtgc
      541 ttggacagcc tagcttgcac gtgggctttc agctcgtcgg ccagtgcacg tttgtcgcgt
      601 tcgcgcgcgt ccgggcgaag caccacgatc gcctttggct tcgtgaggcc ctggtcttct
      661 ccggggatga ccgcgacgac cgagacagcc gggtgcgcga gcaggcagtc ttcgacctcg
      721 cgcggcgcga cgaacacgcc accgaccttg aacagatcgt cggcgcgacc ctggaagtac
      781 aggtagcccg cctcgtcgat cgagaacaga tcgccggtgc ggcaccagtg accatggaac
      841 gtcttccatg atttctcgcg atcgcggaag tagccctgcg agaccgagtc gcccttgacc
      901 cacagcacac cgatgtcgcc gcgcggcagc tcgggcgccc ccggaccgtc cgcgtcctcg
      961 gcgaggatct tgagctcgta gccttcgacg gcgcggccga gcgtgccggg cttcacgtcg
     1021 cccggacggt tactgcagta gatatggaac atctcggccg agccgatgcc gtcgtagatc
     1081 tcgccaccga agcgttcgac gaagcggcgc atcagcggct cgggcagcgc ttcgccggcg
     1141 gagaagtgaa accgcacggt cgacagatcg aggcccggtt cgccacgcgc gcggcgctcg
     1201 tcgtcgagat cgaggatctt gccgagcatg gtcggcacgt tcgtgacgac ggttgccttg
     1261 tagcgctgga tcgcggcggt gatcgattcg gcggtcggtc gctcgctgaa caggccgacc
     1321 gtcgcgccga cggcgaatgg gaaccagagg ttggtgccgg tcgcgtagcc gaagaacagc
     1381 cgcggcacgc tgaccgtgac gtcgtcctcg cgcatgccga tcgtgcgctt cgcgaacacc
     1441 tcggtgttga acgcgaagtc gcgatgcgtg tgcatgttcg ctttgggctt gcccgtcgag
     1501 ccggaagtga acagccagat cgcttgatcg tcgcgatgga tcttcggcag cgggatgtcc
     1561 gaattcttgc cgagctcgat cgcgtactcg agcgtgccgg gctcgcccgg aagcggctcg
     1621 ggatcgtcgc cggtcgccgc gtcgggggaa tccatgatga tcgcgccggc tcgccgttcc
     1681 ttcggcatga cctgggcgag cgctttcacg atcgtgggtg tcgtgatcac gaacgctgcg
     1741 cgcacgtatt cgatcacgta cgcgaggtcc tcgatgggag actgcggatt gcccatcgtg
     1801 acgacgccac ctgccgcgag cgtgccgaag atgctccaga cgaacggcgg cgtatccggc
     1861 agcacgatgt acacgcgctg ttcgggcttg agtcccgcct tgatcagcga gcgggcgagc
     1921 gcccgcgacc gctcggcgac gtcggcgtag gtgtacgagc gttcaccgaa ccgcaacgcg
     1981 actcggttgc ctttgccttc cttcaggcga tcgaacagcc agtaatcggc gaggttgaag
     2041 tcgtctggaa accggacgtc gctcatcgtg caagttcctt cgcgagcgca cgaccgatga
     2101 tcgtgcgctg gatttcactg gtgccttcgt agatccgcag cggccggatc gcgcgataga
     2161 gatgctcgac gatgtgcccg gccatcacgc cgcggccgcc gaacagctgc actgcgcgat
     2221 cgatcacgcg ctgggccgct tccgtcgcgc cgagcttcgc gatcgagacc tcggtcgtca
     2281 cccgctcggc tgtcgtgtcc ttgcgatgcg ccgcgcgcag cacgagcaag cgcgccgtat
     2341 cgaggtcgac cgccatgtcg gcgatgtgcg cctgcacgag ctgttgctcg gcgagcggct
     2401 tgccgaactg gatgcgcttg gtgacaaacg cgatcgcctc gtccatcgcc cggcgcgcca
     2461 taccgaccgc ggccgcaccg accgacacgc ggaacgcgtc gagcgtttgc atcgcaagct
     2521 tcatgccctg cccttcggca ccgatgcgcg ccgattccgg cacgaagaca ttacgcagcc
     2581 cgagcgcacc gatcggatgc ggagctgtcg ccacgagcgg catcacggtg acgcccttgg
     2641 tgtcgagcgg cacccagaac gccgagaggc cctgcgcgcc gcggctctga tccgtcgagg
     2701 cgaacacggt cgcgtgatcg gcgatgccga ggttcgagat gaacaacttg tcgccattca
     2761 gctcgtagcc accggggcgg ggagccgcgt gcgtctggat cgacgcgaca tcgctgcccg
     2821 cctccggctc cgtcacggcg aaggcggcga cctgctggcc gcgcgcaaac gcatcgacct
     2881 gcgcgcgctg ctctgggctg ccggcgagca agatcgagtg cacgccgagg ccttgcaccg
     2941 caaagatcga atccgcgcgc gccgagtgat agccgagcat ctcgcgcgtg aggcacagcg
     3001 agcgtgcgtc cgtcttgccg ctcgctggcg cgacgagctc gaacaagccg ctcttcgcaa
     3061 acgccgcgac cgctgcacga tcgcgcgccg gctcgtcggc agcgagctct gcctcggcga
     3121 acgaggccgc cgctgcgcgc aaccgatcgg cgagcgccac gtgggtggcg tcgaagaaca
     3181 tcggcaggtc gtcggtcgaa agccgcatga ctatttcgcg ccgacaaagt gcggcggccg
     3241 ctcttccttg aacgccgcgt gcgcttcgcc gaagtccggg tgctgcatgc acagcgcctg
     3301 cgcttgcgcc tcggcttcga tcgcggcggc caggggcatc gtgtgctcgc tctcgagcat
     3361 ctgcttcgtc atcgcgtgcg cgaacgccgg accggcggcg agctttgcgg cccattcctg
     3421 cgcgagcgcg agcgccttgt caccgtcctc gacgatgcga ttcgcgagac cgatcttcgc
     3481 ggcatgctcc gcgtcgatca cgtcgccgaa gaacaacagc tcgctcgcgt ggccgaggcc
     3541 gacgatgcgc ggcaacaggt acgtcgcgcc catgtcggcg ccgcacaggc cgaccttcgg
     3601 gaagatgaaa ccgaacttcg cggtggcggc gacgatccgc atgtcacacg cgcacgcgat
     3661 caccgcgcca gcgccgaccg cgatgccgtt gatcgcgcca acgatcggcc ggcggcactt
     3721 gcgcattgcc gcgatcagcc gccccgtggc gcgcgtgaag gacacgagtc cgggcgtatc
     3781 tcgcccgagc aggtgcttga tgatgtcgtc ctgatcgcca cccgagcaga agcctttgcc
     3841 tttgccggtg atgaccaccg cccgaacctc gtcggtccga tcgagctgct cgaagacctc
     3901 ggccagctcg cgatagatct cgaaggtgag cgcgttgaga cgatccggcc gatcgagggt
     3961 gatcgtcgcc accccagcag ccagctcgag cgaaaacgtc tgcgccgtcg gcagtcc
//
LOCUS       DASUFX010000606         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2043584, whole genome shotgun sequence.
ACCESSION   DASUFX010000606 DASUFX010000000
VERSION     DASUFX010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043584"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /gene="lon"
                     /locus_tag="VFV99_24350"
     CDS             <1..1663
                     /gene="lon"
                     /locus_tag="VFV99_24350"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5062535.1"
                     /translation="ELDDGGGDLDEFSEKITKAKMPEEAEKVAKKQLDRLKGMQPSSA
                     EYTVTRTYLEWLVELPWSISTEDHIELAEVRKCLDEDHYDLDKVKKRIVEYMAVRKLK
                     NDKKGPILCLAGPPGVGKTSLGRSVARAIGRKFGRISLGGVRDEAEIRGHRRTYVGSL
                     PGRIVQGIKKAGTNNPVFVLDEIDKLGHDFRGDPASALLEVLDPEQNNTFSDHYLEVT
                     FDLSKVLFIATANQLDPIPWALRDRLEIIELPGYTRQEKKQIARKFLVPKQLDDHGLT
                     NDRCEITDDAIFEIVDSYTREAGVRNLEREIGSVCRAVAVKVAEGQAKEHEVVNGPVV
                     EEVLGPKKFVSEVADRTSEPGVATGLAWTSVGGDILFIEATKMPGKGKLTLTGQLGDV
                     MKESVTAALSFVRGRAAQLGLDPGNFLENTDLHVHVPAGAVPKDGPSAGITMYTALVS
                     LFTGVPVRSDVAMTGEITLRGNVLQIGGVKEKLLAAHRAGIKRVIIPDRNMKDLIDVP
                     EEVKGEMEILSVRRMDEVLAIALKDPPQAIIDIAKAAQVDDHPRA"
     gene            complement(1688..2101)
                     /locus_tag="VFV99_24355"
     CDS             complement(1688..2101)
                     /locus_tag="VFV99_24355"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5062536.1"
                     /translation="MSNINQEMLALERRYWDAVQHKDTEEAISLSDESCIVVGSQGVG
                     AIDREQLGKMLQAASYELKTYAFDDKKFKVRQVADNVAIVAYPVREELIVDGQTTTLE
                     AFDASVWVRRNGKWQCALHTESLKGDPFGRDRQPS"
     gene            complement(2151..3185)
                     /locus_tag="VFV99_24360"
     CDS             complement(2151..3185)
                     /locus_tag="VFV99_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062537.1"
                     /translation="MSVEALVSAVREVAARIGLELQWELAGDDDDLRWLYELHVIVER
                     PGPAHSRYSTLPLHRFAAETLDQREEAYRIASAVAAELGLPLHGPPIEDHGGWGESAW
                     IRAQTKGPLVAYPLRWEASWWTDGGKQNDARGNEHVMADSGTEARFALHRRLAAQFAA
                     RPLTLRLFDGDTLVDNGTLGAPYPRELPPIDEVRATALRSLCRAPEIAKQLVAEVPAL
                     SPRALMVAFEDAFDLWSFDKLAPLAQWRRGEIDDAELDREITPRIADKRADWDRPRVL
                     REAHRAGKSIAAHLREERRAGANMIQLHRALREAFDISFGDAKLFVDDCQDGRNDSRL
                     DAQLKAHPRS"
     gene            3399..>3511
                     /locus_tag="VFV99_24365"
     CDS             3399..>3511
                     /locus_tag="VFV99_24365"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5062538.1"
                     /translation="MGKIIGIDLGTTNSVVAIMEGKEPKVIANEEGARLTPS"
BASE COUNT          625 a         1143 c         1157 g          586 t
ORIGIN      
        1 tgagctcgat gacggcggcg gagatctcga cgagttctcc gagaagatca cgaaggcgaa
       61 gatgcccgag gaggccgaga aggttgccaa gaagcagctc gatcgcctca agggcatgca
      121 gccgtcgagc gcggagtaca cggtcacgcg cacgtacctc gagtggctcg tcgagctgcc
      181 gtggtcgatc tcgaccgagg atcacatcga gctcgccgag gtgcgcaagt gcctcgacga
      241 ggatcactac gatctcgaca aggtgaagaa gcgcatcgtc gagtacatgg cggtgcgcaa
      301 gctcaagaac gacaagaagg gcccgatcct gtgcctcgcg ggcccgccgg gcgtcggcaa
      361 gacgtcgctc ggtcgctcgg tcgcgcgcgc gatcggccgc aagttcggcc gcatttcgct
      421 cggcggcgtg cgcgacgagg ccgagatccg cggtcaccgc cgtacgtacg tcggttcgct
      481 gccgggtcgc atcgtgcagg gcatcaagaa ggcgggcacc aataacccgg tcttcgtgct
      541 cgacgagatc gacaagctcg gtcacgactt ccgcggtgat ccggcgagcg cgctcctcga
      601 ggtcctcgac cccgagcaga acaacacgtt ctccgatcac tacctcgagg tgacgttcga
      661 cctgtcgaag gtcctcttca tcgcgacggc gaaccagctc gatccgattc cgtgggcact
      721 gcgcgaccgt ctcgagatca tcgagctgcc cggttacacg cggcaggaga agaagcagat
      781 cgcgcgcaag ttcctcgttc cgaaacagct cgacgatcac ggcctcacga atgatcgatg
      841 cgagataacc gacgacgcga tcttcgagat cgtcgacagc tacacgcggg aagccggcgt
      901 ccgtaacctc gagcgcgaga tcggcagcgt gtgccgcgcg gtcgcggtga aggtcgccga
      961 gggtcaagcg aaagagcacg aggtcgtcaa cggtccggtc gtcgaagaag tcctcggccc
     1021 gaagaagttc gtcagcgaag tcgcggatcg caccagcgag ccgggcgtcg cgacgggcct
     1081 cgcgtggacg agcgtcggcg gcgacatcct gttcatcgaa gcgacgaaga tgccgggcaa
     1141 gggcaagctg accctgactg gtcagctcgg cgatgtgatg aaggagtcgg tgaccgcagc
     1201 gctgtcgttc gtgcgtggtc gcgcggcgca gctcggtctc gatccgggca acttcctcga
     1261 gaacaccgac ctccacgttc acgtgcccgc gggcgcggtg ccgaaggacg gtccgtccgc
     1321 aggcatcacg atgtacaccg cactggtctc gctgttcacc ggcgtgcccg tgcgttcgga
     1381 cgtcgcgatg accggcgaga tcacgctgcg cggcaacgtg ctgcagatcg gcggcgtgaa
     1441 ggagaagctg ctcgcggctc accgcgccgg catcaagcgc gtgatcattc ctgaccggaa
     1501 catgaaggat ctgatcgacg tccccgaaga agtgaagggc gagatggaga tcctcagcgt
     1561 gcgccgcatg gatgaagtgc tggcgatcgc gctcaaagat ccgccgcagg cgatcatcga
     1621 catcgcgaaa gccgcgcaag tcgacgatca cccgcgcgcg tagcgggtat ccgcagctcg
     1681 cgcgccgtta gctcggttgg cggtcgcggc cgaacgggtc gcctttgagc gactcggtgt
     1741 gcagtgcgca ttgccacttg ccgttgcgcc gcacccagac gctcgcgtcg aatgcctcga
     1801 gcgtcgtcgt ctggccgtcg acgatcagtt cttcgcgcac cgggtaggcg acgatcgcga
     1861 cgttgtcggc gacctggcgc accttgaact tcttgtcgtc gaacgcatac gtcttgagct
     1921 cgtagctcgc ggcttgcagc atcttgccga gctgctcgcg atcgatcgcg ccgacgccct
     1981 gcgagccgac gacgatgcat gactcgtcac tcaggctgat cgcctcctcc gtatccttgt
     2041 gctgcacggc atcccagtag cgacgttcga gtgccagcat ctcctggttg atgttgctca
     2101 tagatcctcc gaaccaacga gcagcaactc gcggaccact gcgtgcaagc tcacgatctc
     2161 gggtgtgctt tgagctgcgc atcgagccgg gagtcgttgc ggccatcctg acaatcgtcg
     2221 acgaacagct tcgcgtcgcc gaacgagatg tcgaacgctt cgcgcagcgc gcggtggagc
     2281 tggatcatgt tggcgccggc gcgacgttcc tcgcgaaggt gggcggcgat gctcttgccc
     2341 gcgcggtgcg cctcgcgcag cacgcgcggt cgatcccaat cggcgcgctt atccgcaatc
     2401 cgtggcgtga tctcgcgatc gagctcggca tcatcgatct cgccgcgccg ccactgcgcg
     2461 agcggtgcaa gcttgtcaaa gctccacagg tcgaacgcgt cctcgaacgc caccattagc
     2521 gcgcgcgggc tcagtgccgg cacctcggcg acgagctgtt tcgcgatctc tggcgcgcga
     2581 cacaacgagc gcagtgcggt cgcgcgcacc tcgtcgatcg gtggcagctc gcgcggatag
     2641 ggcgcgccga gcgttccgtt gtcgacgagc gtatcgccat cgaacagccg cagcgtgagc
     2701 ggacgggcgg cgaactgggc ggcgagccgg cgatgcagcg cgaaccgcgc ctcggttccc
     2761 gagtcggcca tcacgtgctc gttgccgcgc gcgtcgttct gcttgccgcc gtcggtccac
     2821 cagctcgctt cccagcgcag cggatacgct acgagcggac ccttcgtctg cgcgcggatc
     2881 caggccgact ctccccaccc gccatggtcc tcgatcggcg gaccgtgcag cggcaggcca
     2941 agctcggcgg cgacggcgct ggcgattcga taggcctctt cgcgctggtc gagcgtttcg
     3001 gcagcgaagc gatgcagtgg cagcgtcgaa taccggctgt gtgccggtcc cgggcgctcg
     3061 actatgacgt ggagctcgta gagccagcgc aaatcgtcgt cgtcgccggc cagctcccat
     3121 tgcagctcga ggccgatccg cgccgcaacc tcgcggaccg cactgaccag cgcctcgacg
     3181 gacatgaccg gctcatcatg gcacctgtgg catgggcgcc cgcgggtccg gagcagggtt
     3241 caaccgcgcg cctgcgaaac accggacggg tcttcggcgt tgacctccca gaatgcctgg
     3301 gtctgtagct accaaagttg ggggcgtgtc ttgacggccc tttgggggct ccttaggttg
     3361 cccagcaaac aaatcccgca ttcgaggaac aacaagtcat gggcaagatc atcggcatcg
     3421 acctgggcac cacgaactcc gtggtcgcca tcatggaggg caaggagccc aaggtcatcg
     3481 caaacgagga aggcgcgcgc ctgactccgt c
//
LOCUS       DASUFX010000607        15625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2043591, whole genome shotgun sequence.
ACCESSION   DASUFX010000607 DASUFX010000000
VERSION     DASUFX010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15625
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043591"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFV99_24370"
     CDS             <1..388
                     /locus_tag="VFV99_24370"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5062539.1"
                     /translation="ITYWVTRAIKVGAARERPYVHFTSERGGEDNLSFPSGHTSHAFA
                     LAASAGMIAHERGYKLEPYVWASGMTIATLTGYLRIAADRHYLTDVVVGASIGVSAGL
                     TVPFLMRRTNAIVVPSGTGVAFVGEW"
     gene            407..2716
                     /locus_tag="VFV99_24375"
     CDS             407..2716
                     /locus_tag="VFV99_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062540.1"
                     /translation="MRHLISIVVLASSVAHAAPPEPNGAHPRMLLDAELRGQWKAMAK
                     LSAGPVVGAIRLCEDARTTNDHDRAQYQGSEWARMLQACLVAWAATDRNEDAATAIRF
                     FTALLDDRDTIGDGLGGDTAARRDSGYALRNLGPYTALAYDWLYNHPLMTPELKERAR
                     QRWKAWLAWYRDKGYRATNPGSNYHAGYLIAATTIAIAQAGDAAADGTALWQHVADQM
                     WNREMAAALAEEGVLAGGNWPEGWQYGPLSVAEYSLAARVVKRAGVDVPGIETWLSSL
                     LRHHVYALSPSDAMYATGDSEDSDSANLPPNVLTLDAVALGDASAQDKRWARGELARL
                     KLTDRNYFLYNALATVGEKPTLPPRSVWPTWYLSANTGTLYARTRWDDTAIWFVSECH
                     ATIDTDHRHPSAGTFALSRGRDDVIVDPSPYGSASTLTSNAPTVASAQLPADYIPSQG
                     SWSVKTGYDFVTQRASGVVAARCDYSDQYKFQDRPSDVPDAFRDFVLLPSTDGRDAAL
                     LVIDRATTGSDDRGMNLRFRTPGKLALAGDVGTATVGSTKLAITSVTRSAPGTTEIGV
                     PTDGKDCYREGIAKGRCNAARVPVTDYRVLIPGPAPVGVHVISVTGDKGRPATATPLS
                     GKGWAGVHIVGLRDATVVWRTRGSGALDYSGPPGTHVILDGPDVNANVTAQRDAKGCA
                     VHVTGGGTLPARPLVIAVDAECRVKLDPEAPAASALGTRARGTKIPGGMSDARSPRSG
                     CCGAEATPGSAFAMALVVLAILFRRRKGV"
     gene            complement(2745..3662)
                     /locus_tag="VFV99_24380"
     CDS             complement(2745..3662)
                     /locus_tag="VFV99_24380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062541.1"
                     /translation="MKRCLLVLAVVACQSKGDETPPPPKDARVVKVEAPPIDASEEPV
                     REPEPPDPGKVLADLGAVSAWQAAIDRAQYLARRGQHGVVYGTVGTPIMVLGPTPPPA
                     PDAGVNAKLIDAGLVASDYVWLVDDTEGNGSLAIRVALGKFADKAKQGDRVALGGAWT
                     LDEQKRWFWAVDAVTPLPPAPPSDIKDPPAQPGHEIQNGNLAQGSRTISVAKDGDAVY
                     FMLTGPNQPVMPGEGWPVADELGNPIFALLNLPGERPSFGGQDFRTPDERWTLKRGQT
                     YWVRIGKIRKPAPDKPALINARTAPVRVN"
     gene            complement(3659..4144)
                     /locus_tag="VFV99_24385"
     CDS             complement(3659..4144)
                     /locus_tag="VFV99_24385"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5062542.1"
                     /translation="MAETLAISASDKASRYRELFPQLASLVDGEPDLIANLANIAAAL
                     RELLPIASWVGFYIRRGDELVLGPFQGKIACVRIAFGRGVCGTAAAERRTVIVPDVEK
                     FPGHIACDAGSRSEIVVPIVQNGRVVAVLDLDSYEVAAFDDVDAAGLAPIAELAGTFA
                     W"
     gene            complement(4154..4813)
                     /locus_tag="VFV99_24390"
     CDS             complement(4154..4813)
                     /locus_tag="VFV99_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062543.1"
                     /translation="MALPLRAALRSAGRFIAKNPTTMLSMARHALGMRIAVPLDALRW
                     FIANTPPSKKAPQDVTITPRPPAIGIGATVDLMGTTVRAGASIRVDTIEVGEEKFRVS
                     LRLSDVNMKVLGESFTPVAGLIKSGALDLSKPGNLAKFMPKRPAVLVEAHDDVIVLDL
                     MQNPKIAANDRVRGILGTLTPVVQVSGMSTEGDFLILQLRASPFGLPRALNAARQLAA
                     G"
     gene            complement(4841..7282)
                     /locus_tag="VFV99_24395"
     CDS             complement(4841..7282)
                     /locus_tag="VFV99_24395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062544.1"
                     /translation="MHRWVVLLALVGCETPAEPDAVPTTWVYPPSVAAWPIGPLGGTL
                     GRSQAPQLAEQLGIVGNVTVPLRLPTPWAVPGEGPARAVVYGTVGDAPAVEMIDIDAG
                     KILWRDTTECAGPVVGVTEEVIVCADAKGTRAIGLDGKRKWAAEATFIAMTGERVVTA
                     GGGESVILDATLGNELARVKLPVPTALKRPVPKGAPPPPLPVSSESIVASCGDAGREL
                     FAHGQDGQLVRIAEAPGGPKIAWSTPLPLVAAIDACEGATVLVTTATPAGTALVALDR
                     ETGKPTGTVEAVRGFWPARDGSPRIEIATTTSVAIYSRDLAGVPEPTSLPVLDELLAK
                     RGDQRLVRATKYTAALLDARGVRAYVPLAQLGAVLGDKAILAASWLGSPGESVHRYAI
                     PEPYPRKLRVHLQRPGLAVPAELRDLPTPAELEIAGAVAKPDTAKHAVSAVAIDPIEP
                     TAVYAATLEHAPDDTLKAGIARFDLAMRTWRWYRGDGCGAGTPVALAAARAFVACAAR
                     DSKGATVFATTRDGSPLWQWHGTNVDAMQAAADVVLVFDADRVHVLDGRDGKVLATYT
                     SDDGGPMRAAALDVDGMAMVVVFQRGLVMARLPRVQMVPAWTLAVAGAVAALVPAGDG
                     VLVALEDGDAYRVDARTAAIVAMPGLDLLWGASGDVVTGQAPGGPVPPAQLGPGAPKL
                     PAPARRGPPPPPRDPAEDPPVLPKPWPEPPPMLASWQYTLYELTGALRTRNDYALDAP
                     ITPAPRGPGDSPLVVQYGPGLRELLVVEPRRGDPLRRIHLPDDAAPGTAFATLVDGKP
                     VVGTLLFNPLRAVVF"
     gene            complement(7321..8553)
                     /locus_tag="VFV99_24400"
     CDS             complement(7321..8553)
                     /locus_tag="VFV99_24400"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5062545.1"
                     /translation="MADVFIYDALRTPRGRGKAGKGGLANIHPQELLAQTLNHMAERK
                     KLDKNLVEDLAVGCVTQVKEQGACIARVALIAAQWPEDVTGFTINRFCGSGLEAVNSI
                     AAKIGAGFSDSGIGGGVESMSRVPMGSDGAMLDGLNEELRKRLYLVPQGISADLIATR
                     EGISRQDVDKFALASQQKAARAIEGKHFANAMFAVKNPDGSVALERDEHPRADTTLDG
                     LAGLKAAFADMGAMPMGPKGETLDQLALQRYPDTAKIEHVHTGGNSSGIVDGAAVVLM
                     GSAEWGKKAGLRPRAKIRSMATAGAEPVIMLTAPAPASEKALKRAGMSVKDVDLWEIN
                     EAFAVVPMQTARKLGIPEDRINVNGGAIALGHPLGATGAILLGTALDELERANKSTAL
                     ITLCIGGGMGIATVIERV"
     gene            8603..9217
                     /locus_tag="VFV99_24405"
     CDS             8603..9217
                     /locus_tag="VFV99_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062546.1"
                     /translation="MRKLFVGFVLGIVFAACGGKKASNEPPPAQPATGAPIAFEAKSF
                     KTNGKSRGGTVEVKAYNFSDKKLAQYWLLFRYTDATGNVLKVKQGTPFEKDTDFMTLS
                     GNRFKCEPKSWCAFKIDNLEVPEKTAKVEVLARSVTALKDGMHFEDKPLWELNGTEWP
                     GDASAPPAGSAAATPAEGSAAGSGHAAAPPPEGSAGSAGSGSAS"
     gene            complement(9245..9847)
                     /locus_tag="VFV99_24410"
     CDS             complement(9245..9847)
                     /locus_tag="VFV99_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062547.1"
                     /translation="MRFVVVLFVACSGAKPTAKAAQPATTEQPVANGTHSCADAALGL
                     ENATRGVRAPEQAVFDTLRARCLEDVWTARAVDCFATMAEGELGKCSRIMTDAQRETV
                     FAALAGNEGGNIYVTRARLEQLQVGVPECDRFVTAVTTVLSCEKLSLEDRLQLGNETA
                     EFWSLPTNRLSREDKLRISEVCGQSLQSLEQHALDVGCML"
     gene            complement(9950..11056)
                     /locus_tag="VFV99_24415"
     CDS             complement(9950..11056)
                     /locus_tag="VFV99_24415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062548.1"
                     /translation="MALLPCLLLAGCSDPTADDPEPGAEDPGEEQPQDDFDTPVTPPD
                     DGYVPKAMVASRFGVFYQVSRDVLDLYQDPEKGLPEVANHAWLITQSHATAYASRELA
                     DNVHRRADFYYAPAFDIWDAKHMGWQTASDAQLRTWAHNFRDTAIAAHADLFTFNEAP
                     STTGESANIRTKIATIIRAIHEPDAQGRQLWGVVYLTERAATKSSWTSTAPDFFKAID
                     ETSIALVAEHYHSTGFVCSLSESALADHFFAFRKWLVASGEPAKLRIANQKYTVLHSS
                     RFNDGPSGWSGGDATKTTLANYQRALSRISRVTRQTEGGVNRISFAPTTSQITRFGVQ
                     PRITALFRWHYRHTAAQATELPCIDNYGGNCSCQ"
     gene            complement(11208..11774)
                     /locus_tag="VFV99_24420"
     CDS             complement(11208..11774)
                     /locus_tag="VFV99_24420"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HEX5062549.1"
                     /translation="MIVRLAIIVLVAACDQGVKKEPPKPPAPKDAAAPDALVVVPRPI
                     ADCPHNDLVPSQSDALLAMLKRTSCYGPCPVYQVAVYLDGRVEYVGSRYVADCQGTAQ
                     LDAKQLAELTKMFEDKHFFALNGSYNDYDATDASSAEITFQPTPWRKKRIEHYHGDMD
                     APPVLEEIENEFDRIVGTARWTTIISPY"
     gene            complement(11804..12898)
                     /locus_tag="VFV99_24425"
     CDS             complement(11804..12898)
                     /locus_tag="VFV99_24425"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5062550.1"
                     /translation="MASAGIDRWLQGQPAILLANPTAHSGKAADWIRHARALLDDAHV
                     PHKFVATEPDGATIDRVREAIDDGGTRVIIYMGGDGTFAEAAKGILGSRYAADVAMGM
                     LPTGTANDQGKSFGLEAGAWALERNVTVIAEGYTVACDVGRLVIERGLKEIHRDMFFD
                     SFSVGLGAASLATRNRDRERVGRIPGLGMIYRDQLVYIGAVAQRFFESYVVDTKFDLD
                     AVIDGTVHHFPSLLDVIVKNTKIFGGEWIFDANAEADDGRVELVPVIGRRDFVGKLLG
                     TMRHVPVGMDDLEKLGIEHAPAIAGTHLELSVRADGGLLPAAQVDGEEIPAGDRYVID
                     VQARALRLIVPRDHVDPLAAELSGAMRTGE"
     gene            12929..14047
                     /locus_tag="VFV99_24430"
     CDS             12929..14047
                     /locus_tag="VFV99_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5062551.1"
                     /translation="MVSEEHELIRQTVRQFAQTRVEPQAMSHDESGTFNVALLRELGK
                     LGLIGITVPEADGGAGLDATAACIVHDELAYADPGFTLGYLAHALLFVNNFYWAGNEA
                     QRKRYLPKVLSGEHVGAMGMTEPAVGTDVLGLQTTARRDGDFYVINGRKTFITNGNEC
                     DVALIYAKVDDRITTFVVEKAFGGFTTSPKIPKLGMRASTMSELIFDNCRVPAENLVG
                     TEGGGITNMMRNLEIERLGLSAMSLGIARRCLDAMVQYATERRTFGKPLHEHGQIQRY
                     IGDSFAKVEAMRALIYNVAATTGPHQRNRLGTDAAKLFSSTAAKEVADAAIQVLGGYG
                     YCTEYRVEQFWRDAKLIEIGGGTLEAHQKNITRDLTRP"
     gene            complement(14051..14767)
                     /locus_tag="VFV99_24435"
     CDS             complement(14051..14767)
                     /locus_tag="VFV99_24435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062552.1"
                     /translation="MRLVIALVLASAFACGKKSPSATSQCADVAQKGVDAMVARAHER
                     AASAQLPADIRAKIEERTKQLDALAPRLRAVITNRCVDDKWPAEVIECYGKVTSMEES
                     RACRAKLSPEQQAKVQKEELDLYAGAMGPPGFGSAHVPTSPEITKLEAELRDENAKLA
                     AAAKKLEGATTDADRAATKAEMQSLQQQMQATNDELAKARANAASTPQPPGPGSAAAS
                     GSNRPVGSSSEPTGSVGSAK"
     gene            complement(14755..15354)
                     /locus_tag="VFV99_24440"
     CDS             complement(14755..15354)
                     /locus_tag="VFV99_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062553.1"
                     /translation="MNKLMMVIALVLALGACSKKKEGNAGAAIAKMTELKDEMCKCKD
                     AACAQDVSARMQKWASEQPEEQKNRKPSDAERKQLAAIGDEIGKCMATAMTAAPPPPP
                     AGSDTPPPAGSGNAGSADVVAPPQGMPPECAAYKETVEKLKPCDKLPPKAKEALIKAY
                     NDAATGWANLPEGAKAGLKTSCKAGAEAVAEAAKAACGW"
     gene            complement(15441..>15625)
                     /locus_tag="VFV99_24445"
     CDS             complement(15441..>15625)
                     /locus_tag="VFV99_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062554.1"
                     /translation="GMAAPEGSAAGSAEGSAAGSAEGSAAGSAGSAAPAAEGSAAGSA
                     AGSAAPAGEKKDEKKK"
BASE COUNT         2513 a         5387 c         5124 g         2601 t
ORIGIN      
        1 gatcacctac tgggtgacgc gcgcgatcaa ggtcggcgcc gctcgggagc ggccgtacgt
       61 gcatttcacc agcgagcgcg gcggcgaaga caacctgtcg tttccgtcgg gtcacacatc
      121 gcacgcgttc gcgctggcgg cgagcgcggg catgatcgcg cacgaacgcg gctacaaact
      181 cgagccgtac gtctgggcga gtggcatgac catcgccaca ctcaccggct atctacgtat
      241 cgccgccgac cgtcactatc tgaccgacgt ggtcgtcggc gcgtcgatcg gtgtgtcagc
      301 agggctcacc gtgccattct tgatgagacg cactaacgca atcgtcgtcc cgagtggaac
      361 gggcgtagcg ttcgtcggcg aatggtgatg ctacggtttt cgaaccgtgc ggcacttgat
      421 ctcgatcgta gtgctcgcga gcagtgtcgc gcacgcggcg ccgcccgagc cgaatggtgc
      481 gcatccgcgc atgcttctcg acgccgagct gcgtgggcag tggaaggcga tggcgaagct
      541 gtcggccgga ccggtcgtcg gcgcgatccg tctgtgtgaa gacgcgcgca cgacgaacga
      601 tcacgaccgt gcgcagtacc agggctccga gtgggcgcgc atgctccagg cgtgtctcgt
      661 cgcgtgggcc gcgacggatc gcaacgagga cgccgcgacg gcgatccggt tcttcaccgc
      721 gctgctcgac gatcgcgaca cgatcggcga cggtctcggc ggcgatacag cggcgcggcg
      781 cgacagcggc tatgcgctgc gcaacctcgg cccgtacacc gcgctcgcgt acgactggct
      841 ctacaaccat ccgctgatga cgcccgagct caaggagcgc gcgcgtcagc gctggaaggc
      901 gtggctcgct tggtatcgcg acaagggcta tcgcgcgacg aacccgggct cgaactatca
      961 cgccgggtat ctgatcgcag cgacgacgat cgcgatcgcg caagccggcg acgccgccgc
     1021 cgacggcaca gcgctgtggc agcacgtggc tgatcaaatg tggaaccgcg agatggccgc
     1081 cgcgctcgcc gaagaaggcg tgctcgccgg tggcaactgg cccgaaggct ggcagtacgg
     1141 gccgctatcg gtcgccgagt actcgctcgc tgcgcgggtg gtgaagcgcg ccggcgtcga
     1201 cgtgcccggc atcgagacgt ggctatcgtc gctgctgcgc catcacgtgt acgcgctctc
     1261 gccgagcgat gccatgtatg cgaccggtga ctccgaagac agcgacagtg cgaacctgcc
     1321 gccgaacgtt ctcacgctcg acgcagtcgc actcggtgac gcgtcggcgc aggacaagcg
     1381 ctgggcgcgc ggcgagctcg cacggctcaa gctcaccgat cgcaactact tcctctacaa
     1441 cgcgctcgcg acggtcggcg agaagccgac gctgccgccg cgctcggtgt ggccaacctg
     1501 gtacctgtcc gcgaacaccg gaacgctgta cgcgcgcacg cgctgggacg acacggcgat
     1561 ctggttcgtc agcgagtgcc acgcgacgat cgataccgac caccgccatc cgagcgcggg
     1621 cacgtttgca ctgtcgcgtg gccgcgacga cgtcatcgtc gatccgtcgc cgtacggatc
     1681 ggcgtcgacg ctcaccagca acgcaccgac ggtcgcatcc gcgcagttgc cggcggacta
     1741 catcccgagc caaggctcgt ggagcgtgaa gaccggctac gacttcgtca cgcaacgcgc
     1801 gagtggcgtc gtcgccgcgc gctgcgacta ctcggatcaa tacaagttcc aagatcggcc
     1861 gagcgacgtg cccgatgcgt tccgcgattt cgtgctgcta ccgagcaccg acgggcgcga
     1921 tgccgcgctg ctcgtgatcg atcgcgcgac gaccggcagc gatgatcgcg gcatgaacct
     1981 gcgcttccgc acgccgggca agctcgcgct cgccggtgac gtcggcacgg cgaccgtcgg
     2041 cagcacgaag ctcgcgatca cgagcgtcac gcgcagtgca ccaggcacga ccgagattgg
     2101 cgtgccgacc gatgggaagg attgctatcg cgaaggcatc gcgaagggtc gctgcaatgc
     2161 ggcgcgtgtt ccggtcaccg actaccgcgt gctgattccg ggccccgcgc ccgtcggtgt
     2221 tcacgtgatc agcgtgaccg gtgacaaggg ccggccagcg acggcaacgc cgctgtccgg
     2281 caaaggctgg gcgggcgtcc acatcgtcgg gctgcgcgac gcgaccgtcg tgtggcgtac
     2341 gcgcggcagc ggcgcactcg attacagcgg cccgccgggc acgcacgtga tcctcgacgg
     2401 tccggacgtc aacgcgaacg tcaccgcaca gcgcgacgcg aaaggctgcg cggtccatgt
     2461 cacgggtggc ggcaccctgc ccgcgcgccc gctcgtgatc gccgtcgatg ccgagtgtcg
     2521 cgtcaagctc gatcccgagg cgccggctgc atcggcgctc ggcacgcgcg cacgcggcac
     2581 caagattccg ggcggcatga gcgacgcgcg ctcgccgcgc tcgggttgct gcggtgccga
     2641 agcaacgccg ggctcggcgt tcgcgatggc gctcgtcgtg ctcgcgatct tgttccgccg
     2701 cagaaaaggg gtctgaccgt ctgaccccgt tttccgtttt tcccctagtt cacgcggacg
     2761 ggagccgtac gcgcgttgat cagcgccggc ttgtcgggcg cgggcttgcg gatcttcccg
     2821 atgcgtaccc agtaggtctg gccgcgcttg agcgtccagc gctcgtcggg cgtgcggaag
     2881 tcttggccgc cgaagctcgg ccgttcgccg ggaaggttga gcagcgcgaa gatcggattg
     2941 ccaagctcgt cggcgacggg ccagccctcg cctggcatca ccggttggtt cgggccggtc
     3001 agcatgaagt agacggcgtc gccgtccttt gcgaccgaga tggttcgcga gccttgcgcg
     3061 aggttaccgt tctggatctc gtggcccggc tgcgcaggcg gatccttgat gtcactcggc
     3121 ggcgcgggcg gcagcggggt gaccgcgtcg acggcccaga accagcgctt ctgctcgtcg
     3181 agggtccacg cgccgccgag tgcgacgcga tcgccctgct tcgccttgtc ggcgaatttt
     3241 ccgagcgcga cgcgaatcgc gagcgagccg ttgccctcgg tgtcgtcgac gagccacacg
     3301 tagtcgctcg ccacgaggcc ggcatcgatc agcttcgcgt tgacacccgc gtcgggtgcg
     3361 ggaggcggcg tcggcccgag caccatgatc ggcgttccga cggtgccgta gacgacgccg
     3421 tgctgaccgc ggcgcgcgag atactgcgcg cgatcgatcg cggcttgcca tgcggagacg
     3481 gcgccgaggt cggcgagcac cttgcccgga tcgggcggct cgggctcgcg caccggctct
     3541 tcgctcgcgt cgattggcgg cgcctcgact ttgacgacgc gcgcgtcctt cggaggtggt
     3601 ggtgtctcgt cgcctttcga ctgacacgcg acgaccgcga gcacgaggag acagcgcttc
     3661 accatgcgaa cgtgccggcg agctcggcga ttggcgcgag gcctgcggca tcgacgtcgt
     3721 cgaacgcggc gacctcgtag ctgtcgagat cgagcaccgc gacgacgcga ccgttctgga
     3781 cgatcggcac cacgatctcg ctgcgacttc cggcgtcgca cgcgatgtgg cccgggaact
     3841 tctcgacgtc gggcacgatc acggtgcgcc tctcggccgc tgccgtgccg cagacgccgc
     3901 ggccaaacgc gatccgcaca cacgcgatct tgccctggaa cggaccgagc acgagctcgt
     3961 cgccgcgccg gatgtagaag ccgacccagc tcgcgatcgg tagcagctcg cgcaatgccg
     4021 cggcgatgtt ggcgaggttt gcgatcagat ccggctcgcc gtcgacgagc gaggcgagct
     4081 gcgggaacag ctcgcgatag cggctcgctt tgtccgatgc cgagatcgcc agcgtttccg
     4141 ccataaggtc gttctagcct gccgcgagct ggcgcgctgc attgagcgcg cgcggcaagc
     4201 cgaacgggct cgcgcgcagc tgcaggatca ggaaatcacc ttcggtcgac atgcccgaca
     4261 cctgcacgac cggcgtcagc gtaccgagga tgccgcgtac gcggtcgttc gcggcgatct
     4321 tcgggttctg catgaggtcg agcacgatga cgtcgtcgtg cgcttcgacg agcacggccg
     4381 gacgcttcgg catgaacttg gcgaggttgc ccggcttgct aagatcgagc gcacccgact
     4441 tgatgagacc ggcgaccggc gtgaacgact cgccgagcac cttcatgttg acgtcggaga
     4501 ggcgcagcga gacgcggaac ttctcttcgc cgacttcgat cgtatcgaca cgaatcgaag
     4561 cacccgcgcg caccgttgtg cccatgagat cgacggtcgc accgatgccg atcgcaggcg
     4621 gccgcggcgt gatcgtgacg tcctgcggag ccttcttgga cggcggcgtg ttcgcgatga
     4681 accaacgcaa cgcatcgagc ggcaccgcga tccgcatgcc gagcgcgtgg cgcgccatgc
     4741 tcagcatggt ggtgggattc ttggcgatga agcgtccggc ggaacgcagc gctgcacgaa
     4801 gcgggagagc catccctcga tgtatatcag cgaaccgtca ctaaaacacc actgcacgca
     4861 acgggttgaa tagcagcgta ccgacgacgg gtttaccgtc gacgagcgtg gcgaatgccg
     4921 tcccgggagc cgcgtcatcg ggtaagtgaa tgcgccgcag cggatcaccg cgcctgggtt
     4981 cgacaacgag aagctcgcgc agccccggcc cgtattgaac gacaagcggg ctgtcgcccg
     5041 gaccgcgcgg cgcgggagtg atcggcgcat cgagtgcgta atcgttgcgc gtccgtagag
     5101 cgccagtcag ctcgtatagg gtgtattgcc agctcgccag catcggcggc ggctctggcc
     5161 aaggcttcgg caagaccggc ggatcttcgg ccggatcgcg cggtggcggc ggcggaccgc
     5221 ggcgggccgg cgccggcaat ttcggcgcgc ccgggccgag ctgcgccgga ggcaccggtc
     5281 cacccggcgc ctggccggtc acgacgtcgc cgctcgcacc ccagagcaga tccaacccgg
     5341 gcatcgcgac gatcgctgcg gtgcgcgcat cgacccgata cgcatcgccg tcttcgagtg
     5401 cgacaagcac gccgtcgccg gccggcacga gcgccgcaac cgcgccggcg actgcgagcg
     5461 tccatgccgg caccatctga acgcgcggca gccgcgccat cacgaggccg cgctgaaaca
     5521 cgacgaccat cgccatgccg tcgacatcga gcgccgctgc gcgcatcggg ccgccgtcat
     5581 cgctcgtgta cgtcgcgagt accttgccat cgcgaccgtc gagcacgtgc acccgatcgg
     5641 cgtcgaacac gagcacgaca tccgccgccg cttgcatcgc atcgacgttg gtgccgtgcc
     5701 attgccagag cggactcccg tcgcgcgtcg tcgcgaacac ggtcgcgcct ttgctatcgc
     5761 gcgccgcaca tgcaacgaac gcgcgcgccg ccgcaagcgc gacgggcgtt cctgcgccac
     5821 aaccgtcgcc gcgataccag cgccacgttc gcatcgcgag atcgaagcgc gcgatgccgg
     5881 ccttcagggt gtcgtcgggg gcgtgctcga gcgtcgctgc gtacaccgcc gtaggctcga
     5941 tcggatcgat cgcgaccgca ctcactgcgt gcttcgcggt atccggtttc gcgaccgcgc
     6001 cggcgatctc cagctccgcc ggcgtcggca aatcgcgcaa ctcggccggc actgcgagtc
     6061 ccggtcgctg caggtgcacg cgaagcttgc gcggatacgg ttccggaatc gcatagcgat
     6121 gcacgctctc acccggcgaa ccgagccagc tcgcggcgag gatcgccttg tcgccgagca
     6181 ccgctccgag ctgagcgagc ggcacgtacg cgcgcacgcc gcgcgcatcg agcagcgccg
     6241 cggtgtactt cgtcgcgcgc acgagccgtt gatcaccacg cttcgcgagc agctcgtcga
     6301 gcaccggcaa gctcgtcggc tcgggcacgc cggcgagatc gcgcgagtag atcgccacgc
     6361 tcgtcgtcgt cgcgatctcg atgcgtggac tgccatcgcg cgcgggccag aagccgcgga
     6421 ccgcttcgac ggtgccagtc ggcttgccag tttcgcggtc gagcgccacg agcgcagtac
     6481 ccgctggcgt cgcggtcgtc acgagcacgg tcgcgccctc gcacgcgtcg atcgcagcga
     6541 cgagcggcaa cggcgtcgac cacgcgatct tcggtccacc cggcgcttcg gcgatccgga
     6601 cgagctggcc gtcctgcccg tgtgcgaata gctcgcgccc ggcatcgccg cagctcgcta
     6661 cgatcgactc gctactgacc ggtagcggtg gtggtggcgc gcccttcggc accggtcgct
     6721 tgagcgcggt gggcaccggc agcttcacgc gtgcgagctc gttgccgagt gtcgcgtcga
     6781 ggatcaccga ttcgccgcca cccgcggtca ccacgcgctc gccggtcatc gcgatgaacg
     6841 tcgcctcggc ggcccacttc cgcttgccgt cgagaccgat cgcgcgcgtg cccttcgcat
     6901 ccgcgcagac gatcacttcc tcggtcacac cgacgacagg ccccgcgcac tccgtcgtgt
     6961 cgcgccacag aattttgccg gcatcgatgt cgatcatctc gaccgccggc gcatccccca
     7021 ccgtgccgta gacgaccgca cgcgctgggc cctcgcccgg caccgcccac ggcgtcggca
     7081 atcgcagcgg cacggtcacg ttgccgacga tgccgagctg ctcggctagc tgcggcgcct
     7141 ggctgcggcc gagcgtgccg ccgagcgggc cgatcggcca tgccgctacc gacggtggat
     7201 acacccacgt ggtcggcacg gcgtcgggct cggccggcgt ctcacagccg accagcgcca
     7261 gcagcacgac ccagcggtgc acgcgcgcaa actagcacac gcgccgctgg gtcacctcca
     7321 ctacacgcgc tcgatgacgg tcgcgatgcc catgccgccg ccgatgcaca gcgtgatcag
     7381 cgcggtggac ttgtttgcac gctcgagctc gtcgagcgcg gtgccgagca ggatcgcgcc
     7441 ggtcgcgccg agcgggtggc cgagtgcaat cgcgccaccg ttgacgttaa tgcgatcctc
     7501 ggggatgccg agcttgcgcg cggtctgcat cgggacgacc gcgaacgcct cgttgatctc
     7561 ccacaggtcg acgtccttca ccgacatgcc ggcgcgcttc agcgccttct ccgacgctgg
     7621 cgccggcgcg gtgagcatga tcacgggctc cgcacccgcg gtcgccatcg agcggatctt
     7681 cgcgcgcggc ctcaggcccg ccttcttgcc ccactcggcc gagcccatca gcacgacggc
     7741 cgcaccgtcg acgatgcccg acgagttacc gccggtgtgc acgtgctcga tcttcgcggt
     7801 gtcgggatag cgctgcagcg cgagctgatc gagcgtctcg cccttcggcc ccatcggcat
     7861 cgcgcccatg tcggcgaacg ccgccttgag gccagcgaga ccgtcgagcg tggtgtcggc
     7921 gcgcggatgc tcgtcgcgct cgagcgcgac cgaaccgtcc gggttcttca ccgcgaacat
     7981 cgcgttcgcg aagtgcttgc cttcgatcgc gcgtgccgcc ttctgctggc tcgcgagcgc
     8041 gaacttgtcg acgtcctgac gcgaaatgcc ttcgcgcgtc gcgatcaggt cggccgagat
     8101 gccctgcggc acgaggtaca ggcgcttgcg cagctcctcg ttgagaccgt cgagcatcgc
     8161 gccgtcgctg cccatcggca cgcgcgacat cgactcgacg ccgccgccga taccgctgtc
     8221 ggaaaagccc gcaccgatct tcgcggcgat gctgttgacc gcctcgagac ccgaaccgca
     8281 gaagcggttg atcgtgaagc cggtgacgtc ctcgggccac tgcgccgcga tcagcgcgac
     8341 gcgtgcgatg cacgcgccct gctctttcac ctgcgtcacg caaccaacgg cgagatcctc
     8401 gacgaggttc ttgtcgagct tcttgcgctc ggccatgtga ttgagggtct gcgcgagcag
     8461 ctcctgcggg tgaatgttcg ccagcccgcc cttgcccgcc ttgccgcgtc cgcgcggtgt
     8521 gcgtagtgca tcgtagataa acacatcagc catgacagaa cctccggtgt ggatgggcga
     8581 aggttgtgtt accacgcacc cgatgcgcaa gctcttcgtc ggctttgtcc tcggaatcgt
     8641 cttcgccgca tgcggcggca agaaggccag caacgagccg ccgccggctc agcccgcgac
     8701 cggcgcgccg atcgcctttg aagccaagag cttcaagacg aacggcaagt cccgcggcgg
     8761 caccgtcgag gtcaaggcgt acaacttctc cgacaagaaa ctcgcgcagt actggctgct
     8821 gtttcgttac acggatgcaa ccggcaacgt gctgaaggtc aaacagggaa cgccattcga
     8881 gaaggacacc gacttcatga cgctgtcggg aaatcggttc aagtgcgagc cgaagagctg
     8941 gtgcgccttc aagatcgaca acctcgaggt tcccgagaag acggcgaagg tcgaggtcct
     9001 cgcgcgctct gtcacggcgc tcaaggacgg catgcacttc gaagacaagc cgttgtggga
     9061 gctgaacggg acggaatggc ccggtgatgc cagcgcgcct cccgcgggca gtgcagcagc
     9121 gactcctgcc gaaggcagcg ccgccggcag cggccacgca gcggcacctc cgcccgaggg
     9181 cagcgcaggc agcgccggat ccggttctgc gtcctgaggt ctgaggcctg aggcctgagg
     9241 cctgtcagag catgcagccg acgtcgagcg cgtgttgttc gagcgactgc aacgactgac
     9301 cacagacttc cgagatccgc agcttgtcct cgcgcgacag ccggttcgtc ggtagcgacc
     9361 agaactcggc agtctcattg ccgagctgca gccgatcttc gagcgacagc ttctcgcacg
     9421 acagcacggt cgttaccgcg gtgacgaacc ggtcgcactc gggcacgccg acttgcagct
     9481 gctcgagccg agcgcgcgtg acgtagatgt tgccgccctc gttaccagcg agcgcggcga
     9541 acacggtctc gcgttgcgcg tcggtcatga tgcgcgagca cttaccgagc tcgccctccg
     9601 ccatcgtcgc gaagcaatcg acagcgcgcg ccgtccagac gtcttcgagg caccgcgctc
     9661 gcaacgtgtc gaacaccgct tgctcgggtg cacgcacgcc acgcgtcgcg ttctcgagac
     9721 caagcgcagc atcggcacac gagtgcgtgc cgtttgcgac cggctgctcc gttgtcgccg
     9781 gctgtgctgc cttcgcggtg ggcttcgcgc cgctacacgc gacgaagagc acgacgacga
     9841 agcgcatgtt cacaggctag catcgtcgac gataaggtgc ccgtgcgcgt acacgtactc
     9901 ggatgatgat gagggtaact ggcgagtacg cgcacgcgca cgggcggagt tattgacaac
     9961 tacaattgcc cccgtagttg tcgatgcatg gaagctcggt cgcctgtgcc gcagtgtggc
    10021 ggtaatgcca ccggaacagc gctgtaatgc gaggctgcac gccaaaccgc gtgatctggc
    10081 tcgtcgtcgg cgcaaacgaa attcgattaa cgccaccttc ggtctgtcgc gtcacacgcg
    10141 agatccgaga gagcgcacgt tgataattgg ccagcgtcgt cttcgtggca tcgccgcccg
    10201 accagccgct cgggccgtcg ttgaaccgag acgaatgcag caccgtgtac ttctggttcg
    10261 cgatccgcag cttcgccggc tcgccgctcg cgacgagcca cttgcggaac gcgaagaagt
    10321 gatcggcgag cgcgctctcc gagagcgagc agacaaagcc ggtcgagtga tagtgctcgg
    10381 cgaccagcgc gatgctcgtc tcgtcgatcg ccttgaagaa atcgggtgca gtcgaggtcc
    10441 acgagctttt cgtcgcggcg cgctcggtca gatagacgac gccccacagc tggcggccct
    10501 gcgcgtcggg ttcgtgaatc gcgcggatga tcgtcgcgat cttcgtgcga atgttcgcgc
    10561 tctcgccggt cgtgctcggc gcttcgttga acgtgaacag atcggcatgc gcggcgatcg
    10621 cggtgtcgcg gaagttgtgc gcccacgtgc ggagctgcgc atcgctcgcg gtctgccaac
    10681 ccatgtgctt cgcgtcccag atgtcgaacg cgggtgcgta atagaagtcg gcgcggcgat
    10741 gcacgttgtc ggcgagctct ctgctcgcgt acgctgtcgc gtgactctgc gtgatcaacc
    10801 acgcgtggtt tgcgacctcc ggcagaccct tctcggggtc ttgatagaga tcgagcacgt
    10861 cgcgcgacac ttgatagaag acgccgaatc gcgacgcgac cattgccttc ggcacgtagc
    10921 cgtcgtcggg cggcgtcacc ggcgtgtcga agtcgtcctg gggctgctct tcgccgggat
    10981 cctccgcgcc gggctcggga tcgtcggccg ttggatcact gcaacctgca agcagcaggc
    11041 atggcagcag ggccatggca aaaccggttc gcatcggtgc gcgcctatca gcaagctctg
    11101 ggccgaacga ggctgattcg caaacccgcg cacctacacg gcgggacatt gccaccgtga
    11161 tgaccatgat catcaagtgc aaacgcgcgt gtgctcacac cctgccgtca atacggcgag
    11221 atgatcgtgg tccagcgcgc ggtgccgacg atgcgatcga attcgttctc gatctcctcc
    11281 agcaccggcg gcgcatccat gtcgccgtga tagtgctcga tgcgtttctt gcgccacggc
    11341 gtcggctgga acgtgatctc ggcgctcgaa gcgtcggtcg cgtcgtagtc gttgtacgac
    11401 ccgttcagcg cgaaaaagtg cttgtcctcg aacatcttcg tcagctcggc gagctgcttc
    11461 gcgtcgagct gggcggtgcc ttggcagtcc gcgacgtagc gcgagccgac gtattcgacg
    11521 cgcccatcga gatagaccgc gacttgatac accgggcacg ggccgtaaca cgacgtccgc
    11581 ttcagcatcg cgagcagcgc gtcggattgg ctcggcacga gatcattgtg cggacaatcg
    11641 gcgatcggtc gcggcacgac gacgagcgca tcgggtgcgg ccgcgtcctt cggcgccggc
    11701 ggctttggcg gctccttctt cacgccctga tcacacgccg cgacgagaac gatgatcgcg
    11761 agacgcacga tcaccagaca ccacctcgcc gcccaggttt cgctcattcg ccggtccgca
    11821 tcgcgccgct tagctcggcg gcaagcggat cgacatgatc gcgcggcacg atcaaccgca
    11881 gcgcacgcgc ttggacatcg atgacatagc gatcgccggc gggaatctct tcaccgtcga
    11941 cctgcgcggc cggcaggagg ccaccgtcgg cgcgcacgct cagctcgagg tgcgtgcccg
    12001 cgatcgccgg cgcgtgctcg atgccgagct tctcgagatc atccatgccg accgggacgt
    12061 ggcgcatcgt gccgagcagc ttgccgacga agtcgcggcg gccgatcacc ggcacgagct
    12121 cgacgcggcc atcgtcagct tcggcgttcg catcgaagat ccattcgccg ccgaagatct
    12181 tcgtgttctt gacgatgacg tcgagcaggc tcgggaagtg atgcacggtg ccgtcgatca
    12241 ccgcgtcgag atcgaacttg gtgtcgacga cgtagctctc gaagaaccgc tgcgcgaccg
    12301 cgccgatgta gacgagctga tcgcgataga tcatgccgag cccaggaatg cggccgacgc
    12361 gctcgcgatc gcggttgcgc gtcgccaggc tcgccgcgcc gaggccgacc gagaacgagt
    12421 cgaagaacat gtcgcgatgg atctctttga gcccgcgctc gatcaccagg cggccgacat
    12481 cgcaggcgac cgtgtagccc tcggcgatca ccgtgacgtt gcgctcgagc gcccatgctc
    12541 ccgcctcgag cccgaacgac ttgccttgat cgttcgcggt gccggtcggc agcatgccca
    12601 tcgcgacgtc cgcggcatac cgtgatccca gtatgccctt cgcggcctcg gcgaacgtgc
    12661 cgtcaccgcc catgtagatg atgacgcggg tgccgccgtc atcgatggcc tcgcgcacgc
    12721 gatcgatcgt cgcgccatcg ggttcggtcg cgacgaactt gtgcgggacg tgcgcatcgt
    12781 cgaggagcgc acgcgcatgg cggatccaat cggctgcctt acccgaatgt gcggtcgggt
    12841 tcgccagcag tatggccggc tggccttgca accaacgatc gatccctgcg ctcgccatcg
    12901 ctgaccgaaa tagtacggta ggctccgcgt ggtgtcagaa gagcacgagt tgattcgcca
    12961 gacggttcgc cagttcgcgc aaacgcgcgt cgaacctcag gcgatgtcgc acgatgaatc
    13021 cggcacgttc aacgtcgcgc tactccgcga gctcggcaag ctcggcctca tcggtatcac
    13081 cgtgccggaa gccgatggcg gcgccgggct cgatgcgacc gcagcatgca tcgtccacga
    13141 cgagctcgcg tacgccgacc ccggcttcac gctcggctac ctcgcccacg cgctcttgtt
    13201 cgtcaacaac ttctactggg ccggcaacga ggcccagcgc aagcgctacc tgccgaaggt
    13261 gctcagcggc gagcacgtcg gcgcgatggg catgaccgaa cccgccgtcg ggaccgacgt
    13321 gctcggcctg cagaccaccg cgcgccgcga tggtgacttc tacgtgatca atggccgcaa
    13381 gacgttcatc acgaacggca acgagtgcga cgtggcactc atctacgcga aggtcgacga
    13441 tcggatcacg acgtttgtcg tcgagaaagc gttcggcggc ttcaccacga gcccgaagat
    13501 tccgaagctc ggcatgcgtg cgtcgacgat gagcgagctg atcttcgaca actgccgcgt
    13561 gccggccgag aacctcgttg gcaccgaagg cggcggcatc acgaacatga tgcgcaacct
    13621 cgagatcgag cggctcggcc tatcggcgat gagcctcggc atcgcacgcc gctgcctcga
    13681 cgcgatggtg cagtacgcga cggagcgccg gacatttggc aaaccgctcc acgagcacgg
    13741 ccagatccag cgctacatcg gcgacagctt cgcgaaggtc gaagcgatgc gcgcgctgat
    13801 ctacaacgtc gccgcgacga ccggcccgca tcagcgcaac cggctcggca ccgatgcggc
    13861 gaagctgttc tcatcgactg ccgcgaaaga agtcgccgac gcggcgatcc aggtactcgg
    13921 tggctacggc tactgcaccg agtatcgcgt cgagcagttc tggcgcgacg ccaagctgat
    13981 cgagatcggc ggcggtacgc tcgaagcgca ccagaaaaac atcacgcgcg atctcacgcg
    14041 cccctaacgg ctacttcgcc gagccgacgc ttccggtcgg ctcactcgag ctaccaaccg
    14101 gtcggttgct tccactcgct gcggcgctcc ccggtcccgg cggttgcggc gtgctcgctg
    14161 cgttcgcgcg cgcctttgcg agctcgtcgt tcgtcgcctg catctgctgc tggaggcttt
    14221 gcatctcggc cttcgtggcc gcgcgatcgg cgtcggtcgt cgcgccctcg agtttcttgg
    14281 ctgccgccgc gagcttcgcg ttttcgtcgc gcagctcggc ttcgagcttc gtgatctccg
    14341 gtgacgtcgg cacgtgcgca gagccgaagc cgggcggtcc catcgcaccg gcgtacagat
    14401 cgagctcttc cttctgcacc tttgcctgct gctcgggcga tagcttcgcg cgacacgcgc
    14461 gcgactcttc catgctcgtc accttcccgt agcactcgat cacctccgcc ggccacttgt
    14521 cgtcgacgca gcgattcgtg atcacagcac gcaggcgcgg cgcgagcgcg tcgagctgct
    14581 tcgtgcgctc ttcgatcttc gcgcgtatgt cggccggtag ctgcgcgctc gccgctcgct
    14641 cgtgtgcgcg cgcgaccatc gcgtcgacgc ccttttgcgc gacgtccgcg cactgcgacg
    14701 tcgcgctcgg cgacttcttg ccacacgcga acgcgctcgc gagcaccagc gcgattacca
    14761 gccgcacgct gctttcgcgg cttcggccac tgcttcggcg cctgccttgc acgaggtctt
    14821 gagaccagcc ttcgcgccct ccggcagatt cgcccagccg gtcgccgcgt cgttgtacgc
    14881 cttgatcagc gcttccttcg ccttcggtgg cagcttgtcg cacggtttca gcttctcgac
    14941 ggtctccttg taggccgcgc attcgggcgg cattccctga ggaggcgcga cgacgtcagc
    15001 actgcctgca ttgccactcc ccgcaggcgg cggcgtatcc gagccagcgg gcggcggcgg
    15061 gggcgccgca gtcatcgcgg tggccatgca cttgccaatc tcgtcgccga tcgcggccag
    15121 ctgcttccgt tcggcgtcgc tcggcttgcg gttcttttgt tcctcgggct gctcggaggc
    15181 ccacttctgc atccgcgcgg agacgtcctg cgcgcacgcg gcgtccttgc acttgcacat
    15241 ctcgtctttg agctcggtca tcttcgcgat cgcagcgccc gcgttgcctt ccttcttctt
    15301 gctgcacgcg ccgagcgcga ggacgagcgc gatgaccatc atgagcttgt tcattgacgt
    15361 ctcctttgcg cgctcattgt agccgccgaa agtaaaaacg ggcccccgag ggagcccgca
    15421 tcaatcagtc gcgaagagag ttacttcttc ttctcgtcct tcttctcgcc agccggggcc
    15481 gccgagccag ccgccgaacc agccgccgag ccctcagccg ccggggccgc cgagccagcc
    15541 gaaccagccg ccgaaccctc agccgagcca gccgccgaac cctcggccga accagccgcc
    15601 gagccttccg gagccgccat gcccg
//
LOCUS       DASUFX010000608         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_516103,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000608 DASUFX010000000
VERSION     DASUFX010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516103"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFV99_24450"
     CDS             complement(<1..344)
                     /locus_tag="VFV99_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062555.1"
                     /translation="MRWLWIALLVGACGSGSSTIDSSGGGDDDAPNIDATAAPTRLVA
                     YIVGGADIAWYDVDKSTGALTAISSTPGWRTGANFLAFHGNYLYAVASGNRAGAYSID
                     PSTASLTFINDVA"
     gene            complement(378..506)
                     /locus_tag="VFV99_24455"
     CDS             complement(378..506)
                     /locus_tag="VFV99_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062556.1"
                     /translation="MKRWRAHQLIATTKTVDFVTSVALDARGAIVCASYDGAISIA"
     gene            770..2803
                     /locus_tag="VFV99_24460"
     CDS             770..2803
                     /locus_tag="VFV99_24460"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062557.1"
                     /translation="MVVDFAAVFDASPNAYMLVDRDLRYVAVNKAYCEATHRRAELLV
                     GTSVMAEFPHDPEAPGNNQARRLADSLRGVFATGTPDVLPLIHYRIEVDGVWQDMYWS
                     ATHTPLFSTDGTVEYVLQHTVNVTEIQRNRSTNLQIEAGILDRAQAGEVRTAELDRSL
                     RDLLSVFDQAPGFLAVTRGPEHVFELANPGYRKLVGGRDVVGRTVRDLLPEVEPHFFE
                     LLDRVYATGEPFVGNGVPVTLANAAGEPTEVFVDFIYQPLSGPDASSRGLLISGYDVT
                     ARVHAERAREHAQAERATLLEAERAARAQAERASLLMDQFLATVSHELRTPLTAILGW
                     TQMLRSGTLAPEKHARALATVERNAQMQAQLVEDLLDVSRIITGKLTMEMEPTDLAGT
                     VAAAIDTVRPSAQEKKVALDIEIDAGGTTVNGDGARLQQVVWNLMSNAVKFTPSEGRV
                     SLRVSRDRDAVEIRVSDTGIGIAPEFLPHVFDRFRQADAGSTRRRGGLGLGLSIVHHV
                     VEAHGGTVRAESKGVNQGSTFIVRIPSASTRTLRAVPTTIAAPGSLHGMKILVVDDEA
                     DTREFVRNLLEQHAATVTAVASAADAITALQHDKPDVLLSDLGMPDEDGYGLIRQVRA
                     LSRECGGRVPAVALTAYARTEDRTRAILAGFQTHVAKPVVPGELLAVLASFRN"
     gene            3110..>3888
                     /locus_tag="VFV99_24465"
     CDS             3110..>3888
                     /locus_tag="VFV99_24465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5062558.1"
                     /translation="MDALAQFKENQKKAWSTFAPTEVYTCQPAARLVTFAQIKAGQRV
                     LDVGCGTGVVALAAARTGAHVTGLDLTPELIVRARENAALAGAEINWHEGDAEALPFG
                     DASFDVVVSQFGHMFAPRPDVATREMLRVLKPGGTIAFTTWPPELWTGRMFALVGRYL
                     PPPPGVSPPPQWGVVEIVRERLGAAVTDLVTDRGTLRAPYLSVQHVRAFMEVNVGPVM
                     RILQTFANEPARIAEFRRELDLLTAQYFETTENVLRQDYLLT"
BASE COUNT          680 a         1266 c         1271 g          671 t
ORIGIN      
        1 gcgacatcgt tgatgaacgt gaggctcgcg gtgctgggat cgatcgagta cgcgccggcg
       61 cgattgcccg aggcgaccgc gtagagatag ttgccgtgga acgcgagaaa gttggcgccg
      121 gtgcgccagc cgggagtcga cgagatcgcg gtaagcgcgc cggtcgactt gtcgacgtcg
      181 taccaggcga tatcggcacc gccgacgatg tatgccacga gccgagtcgg cgccgcggtc
      241 gcgtcgatgt tgggagcgtc atcgtcccca ccgccgctgc tgtcgatcgt cgatgagcca
      301 ctgccgcagg cgccgacaag cagcgctatc cacaaccaac gcatggccgg agcataacgc
      361 catttgttcg tgacggccta cgcgatcgag atcgcgccgt cgtagctggc gcacacgatc
      421 gcgccgcgtg catcgagtgc gacgctcgtg acgaagtcca cggtcttcgt cgtcgcgatc
      481 agctgatgag cgcgccagcg tttcacgcag ccatcctccg ccgcggacac ccacgtacca
      541 tcgctcgcga tggcaaggct cgtgatggct ccgtcgtgtg cgcgagccac ggctaactcc
      601 tcaattcggg cggccaacgc ggcagttccc gcgcctcgcg atctcgttgg gctggccaat
      661 gctccgatcg cgcacgcaag aaacgcgtcg agcgcatacg cacaacgagt ctttcgttcg
      721 cgcacgaaag ccgctggggg atccaggacg cgagtgctaa ctgcagaaga tggtcgtcga
      781 tttcgccgcg gtgttcgatg cgtcaccgaa cgcgtacatg ctcgtcgatc gcgacctgcg
      841 ctacgtcgcg gtgaacaaag cgtactgcga ggccacgcac cgtcgcgccg agctcttggt
      901 tgggacgtcg gtgatggcgg agtttccaca cgatccggag gcaccgggta acaaccaggc
      961 gcgccggctg gccgattcgt tgcgcggcgt gttcgcgacg ggcactcctg acgtgttgcc
     1021 attgatccac tatcggatcg aggtcgatgg cgtctggcag gacatgtact ggagcgcaac
     1081 gcacacgcca ctgttcagca ccgacggcac cgtcgagtac gtgttgcagc acacggtgaa
     1141 cgtcaccgag atccaacgca atcgctccac gaatctgcag atcgaagccg gcatcctcga
     1201 tcgcgcgcaa gccggcgaag tgcgcactgc cgagctcgat cgcagtctgc gcgacttgct
     1261 ctctgtattc gatcaagcgc cggggttcct cgcggtcacc cgcggccccg agcatgtgtt
     1321 cgagctggcg aatccgggat atcggaagct cgtcggcggt cgcgacgtcg tcggccgcac
     1381 ggtgcgcgat ctgttgccag aggtcgagcc ccatttcttc gagctcctcg atcgcgtgta
     1441 cgcaaccgga gaaccgttcg tcggcaacgg cgtgccggtt acgctcgcga acgcagcagg
     1501 cgagcccacc gaggtgttcg tcgatttcat ctatcagccc ttgagcggtc ccgatgccag
     1561 ctcgcggggc ctcctgatct ccggttacga cgtcacggct cgcgtccatg cggagcgcgc
     1621 acgcgagcac gcgcaggcag agcgcgccac gttgctcgag gccgagcgcg ccgcacgcgc
     1681 acaagcggaa cgcgcaagcc tcctcatgga tcagttcctt gcaacggtca gccacgagct
     1741 acgtacacca ctgacagcga tcctcgggtg gacccagatg ctgcggagcg gcacgttggc
     1801 gcccgagaag cacgcgcgtg cgctcgctac ggtcgagcgc aatgcgcaga tgcaagcgca
     1861 gctcgtcgag gatcttctcg acgtcagtcg catcatcacc ggcaagctca cgatggaaat
     1921 ggagccgacc gatcttgccg gaacggtggc ggcggcgatc gatacggtgc ggccatcagc
     1981 acaggagaaa aaggtcgcac tcgatatcga aatcgacgcc ggcgggacga ctgtcaacgg
     2041 cgacggtgcg cgcctccagc aggtcgtgtg gaatttgatg tccaatgccg tcaagttcac
     2101 gccttcggag gggcgcgttt cgctccgcgt ttcgcgtgat cgcgatgcgg tcgagatccg
     2161 tgtctcggat accggcatcg ggatcgcgcc cgaattccta ccgcatgtgt tcgatcgctt
     2221 tcgtcaggcc gacgccggca gtacacgtcg ccgtggtggg ctcgggctgg ggctatcgat
     2281 cgttcatcac gtcgtcgagg cacatggcgg gacggtgcga gccgagagca aaggcgtcaa
     2341 ccaaggctcc acattcatcg tgcgcatccc gagcgcgagc acgcgcacac tgcgagcggt
     2401 tccgacgacc atcgcagcac cagggtcgtt gcacggcatg aagattctcg tcgtcgacga
     2461 cgaagccgac acccgcgagt tcgtgcgcaa cctgctcgag cagcacgccg cgaccgtcac
     2521 cgcggttgca tcggccgcgg atgccattac ggccctgcag cacgacaagc cggacgtgct
     2581 gctgtccgat ctcggcatgc ctgacgagga tggctacggc ctgatccgcc aggtgcgcgc
     2641 attgtctcgc gagtgcggcg gtcgcgtacc agctgtcgcg ctgactgcgt acgcgcgaac
     2701 ggaagatcgc acccgcgcga ttctcgccgg gtttcagacc cacgtggcca agccggtcgt
     2761 gccgggcgag ctccttgccg tgctcgcgtc gttccgcaac tgacaggcgg cggctgcacc
     2821 cagcaccgca atcccacact gctaggcagg cgtcgcgagc agcttgacga gctcggcggg
     2881 taacacgagt tgtggtcgag aattcggctt gatgatcacg cctgcatagg gcgtgcgcat
     2941 cacctcgtcg atcgacgcgc gcttatcagc gcgcgcaggc tctctcgtgg cgtccgctcc
     3001 ggagacgtag gaggcatcgc gtcactctat taccgaagcg accgttgacg taaccgaggc
     3061 ggcgcgcgta cgtatgcctg tcgaacaatt cgctaccgct ggagggaaga tggacgcgct
     3121 cgctcagttc aaggagaacc aaaagaaggc ctggtcgacg ttcgcgccga ccgaggtcta
     3181 cacgtgccaa ccggctgcgc gcctcgtgac gttcgcgcag atcaaggcgg gtcagcgcgt
     3241 gctcgatgtg ggttgtggca ccggcgtcgt cgcactcgcg gccgctcgta cgggcgcaca
     3301 tgtgacgggg ctcgatctca cgcccgagct gatcgtgcgt gctcgcgaga atgccgcgct
     3361 ggccggcgcg gaaatcaact ggcacgaggg cgatgcggag gcacttccgt tcggcgacgc
     3421 gagcttcgac gtcgtcgtca gccagttcgg tcacatgttc gcgccgcgcc ccgacgtggc
     3481 gacgcgcgag atgctccgcg tactcaagcc aggaggcacg atcgcgttca cgacgtggcc
     3541 accggagctg tggacgggcc ggatgttcgc gcttgtcggt cgctacttgc cgccaccgcc
     3601 gggcgtatcg ccgcctccgc aatggggtgt cgtcgagatc gtgcgcgagc gccttggtgc
     3661 cgcggttacc gatctcgtca ccgatcgcgg cacgctgcgc gcaccgtatc tgagcgtgca
     3721 acacgtccgc gcgttcatgg aggtcaacgt cggtcccgtc atgcgcatcc tgcagacgtt
     3781 tgcgaacgag cccgcgcgca tcgccgagtt ccgccgcgag ctggacctgc tgaccgcgca
     3841 gtacttcgag acgaccgaga acgtgcttcg acaggactac ctgctgac
//
LOCUS       DASUFX010000609         7242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_653344,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000609 DASUFX010000000
VERSION     DASUFX010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653344"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFV99_24470"
     CDS             complement(<1..1008)
                     /locus_tag="VFV99_24470"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX5062559.1"
                     /translation="MWLIAMAGCATELSTSEVEQHAGDYQAGDYQSGDYQAGDYQAGD
                     YQAGDYQAGDYQGAHYSSSSFSGATLNGSALTDISITGTTLMVWKKIKASVSPYYGWE
                     QRTPNRICTWNADRSHATCTIVDPSTTASPLAGARFKTTLRTATGTMTGWVQIGASSS
                     ALNAVKPDSSYAMWMLTNIHNNGPYSYSANAPVNCTNAAGCRQNNDLWTYDLRLINAD
                     GSQVPFCSSGQSYALKGTWDSLGEHAASSTDFSFACSTGTIAKCTRWGYRPWITSYTK
                     SDGSTVSFDNFHQACVRAAMADYCTNGHSFTKFHTLVDIYDYAGFIPSMQALYPTTTA
                     FI"
     gene            1360..1947
                     /locus_tag="VFV99_24475"
     CDS             1360..1947
                     /locus_tag="VFV99_24475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062560.1"
                     /translation="MKRRLLVPIVTAGMALASGVASAQTNAHTLEYFRNYGLLLEVSA
                     DPRDGSDFVQTNPTRAGKLELVALNDSVRLRGMTVQFSDGRTFSQPLRTIGVGERVMV
                     DLPANCGLIQSVRLDYGRQIVDRTPARLQIIPHDEQRFTQPTYPRNDRWSRYQQPSHR
                     TYSSRPTYQQPSYVVRPAPQPSWSALGTIQGSFRF"
     gene            2054..3109
                     /locus_tag="VFV99_24480"
     CDS             2054..3109
                     /locus_tag="VFV99_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062561.1"
                     /translation="MQRALAVLLFVVPVAACGGSSECDPNTESCTYEADVSTLTVAAG
                     AEDEDTCQSWTLNNPTELWVNAITQTNNGGYHHANWFFVPDDQFNLPDGTWSCEQNQF
                     GELIAALQGGYLFALSTQSAAETQTLPHNSAIRIPPYSRLIGSSHLLNTSDEDVTTTM
                     HLAITTVPPSEVKAKLAPARMSYHALTLEPQTKSAFTMECDLQKAYDDVWHKPLVYTL
                     YYVLVHYHSLGIYQQLEIMGGPRDGEVIMHHDGFGDNAGHPIEPPLDLAAAGATGLRL
                     TCGFNNPRTAQVGWGIGDQEMCVMALQATTNIGWDADVTQGALTGTSATGEMLHSGAC
                     SVVSFPWDFDKPGGQGH"
     gene            3109..4224
                     /locus_tag="VFV99_24485"
     CDS             3109..4224
                     /locus_tag="VFV99_24485"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5062562.1"
                     /translation="MRVAVVGGGIFGVTAALALRARKLDVRLYDSELPHPLAESTDIS
                     KVVRMDYGADADYTQLGEQALAGWRQWNARWARPLFHETGVTFLSREPMQPGGFEYES
                     YTLLRRRGHALERLDADAIADRLPAWRRGACFDGYFNPQGGWAESGAVVAALAAEAET
                     AGVVIERVRLASLDALDAELVIVCAGSWVPLLVPALAPVLRAVGQPVFHLRPADPSLF
                     EAARFPVFGADISRTGYYGFPVNRDGIVKIANHGVGLTIAPGDTRPDVSDAHEAALRE
                     MLRDTFPALVDAPIVGRRLCVYCDTVDENFWIARDPARPNVVVATGGSGHAFKFAPVL
                     GDLIAAIALGEPHLLAHKFRWRPELAAHGGEAARHHG"
     gene            4321..4623
                     /locus_tag="VFV99_24490"
     CDS             4321..4623
                     /locus_tag="VFV99_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062563.1"
                     /translation="MLTAITLVIAGCGGDDGALPGEPIGATCEMNIDCQTGVCWDFHD
                     HDMLCAGKVCSTACTSDAECVDAASKHGAFGPSNAHCGSDGLCDLVGTGLGTFACQ"
     gene            complement(4626..5636)
                     /locus_tag="VFV99_24495"
     CDS             complement(4626..5636)
                     /locus_tag="VFV99_24495"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5062564.1"
                     /translation="MRASTVRRFVEYIRRYHELSTRALLREAAIRSAQVAKPDGWISV
                     KRTAELLDTAAMKTNNAALTMTFAAQLPWADYGVAAYVVLNSPTVGAALANACRYFAL
                     HSTGAGTQLVVEGQDAFVRYAMHDPTVVRHAQNTEMVFTVYVRAIRDGTGDPRWAPRA
                     IHFKHEKPKNSSTQRDFFGCELLYGQAEDAIIVEPDELRRPMRRADAGLFPILVVHAN
                     AGLPHYLDRDVHDRARSIVASSLRTGDVSIEHVATELGTSERTLQRRLQDRGTSFKQL
                     VADTRLSLARDYLADRGMSLTDAAYLLGYSELSAFSRAFRRWTGESALAFRRRVARRP
                     PE"
     gene            complement(5916..7211)
                     /locus_tag="VFV99_24500"
     CDS             complement(5916..7211)
                     /locus_tag="VFV99_24500"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5062565.1"
                     /translation="MSLDSSWSSRGESAADVAARVPSGARVFVHGAAATPIPLLDALA
                     ARTNVEDVRLYHLHTQGTDSFVAPSVTERLRSISFFVGANARGAVEAGRADFVPVFLS
                     DIPALFSSGAVPIDVALVQLSPPDRHGYCSLGTSVDAARAAVDCAGVVLAEINDRMPR
                     THGNTSVHIDHVHAFVRTNRALPEHAAAAPTAVETRIGELVAQLVEDGATLQTGIGAI
                     PEAVLARLGNRVDLGVHTEMFSDGMIPLIEGGVITNRHKAVHPNRSVTSFVNGTRALY
                     DFVDDNAAIEFHGCDRTNDTALIRKNEKMTAINSALEIDLSGQVCADSIGHRIYSGIG
                     GQMDFLRGAALSRGGKPIIAMPSTAARGSVSRIVAELKPGAGVVTTRGHVHWVVTEYG
                     ARNLHGLTLRERGEAMISLAHPDARGELQRVLASVRHFT"
BASE COUNT         1283 a         2401 c         2297 g         1261 t
ORIGIN      
        1 gatgaacgcc gtcgtcgtcg gatacagcgc ctgcatgctc gggatgaagc ccgcgtaatc
       61 gtagatgtcg acgagcgtgt ggaacttcgt gaacgaatgg ccgttcgtgc agtagtccgc
      121 catcgcggcg cgcacgcacg cctggtggaa gttgtcgaac gacaccgtcg agccgtcgga
      181 ttttgtgtag ctcgtgatcc acggccgata accccagcgc gtgcacttgg cgatcgtgcc
      241 ggtcgaacac gcgaacgaga agtcggtgct gctcgcggcg tgctccccga gactgtccca
      301 tgttcccttc agcgcgtagc tctgacccga cgagcagaac gggacctgag agccgtcggc
      361 attgatcagg cggagatcgt aggtccagag gtcgttgttc tggcggcagc cggcggcgtt
      421 cgtgcaattg accggcgcgt tcgccgagta cgagtacgga ccattgttgt ggatgttcgt
      481 cagcatccac atcgcatacg aagaatctgg cttgaccgcg ttgagcgcgc tcgagctggc
      541 gccgatctgc acccagcccg tcatcgtgcc ggtcgcggtg cgcaacgtcg ttttgaagcg
      601 tgcgccggcg agcgggcttg cggtcgtgct cggatcgacg atcgtgcaag tcgcatggct
      661 cctgtccgcg ttccaggtac agatgcggtt cggcgtgcgt tgctcccacc cgtaatacgg
      721 cgagaccgac gccttgatct tcttccacac catgagcgtg gtgccggtga tcgagatatc
      781 ggtcagcgcg gagccgttca gtgttgcacc agagaacgat gacgacgagt agtgggcgcc
      841 ttgatagtcg cccgcttggt aatcacccgc ctggtagtcg ccggcctggt agtcgccggc
      901 ctggtaatcg cccgactggt aatcgccggc ctgatagtcg ccagcatgtt gctcgacctc
      961 agaagtcgaa agctcggtcg cgcatccagc catcgcaatc aaccacaccc atcgcttcat
     1021 atgacctcgc tgagccaacg cgtataacac ggtccccgac ggggccagac atgagtgttt
     1081 cgcaacggct ctcacagtga ctacgtagtg ccagctcgtg ttcggttgtg acttcagatc
     1141 gacacaaccg ccgaaacgac ctcgaactgg agcgcaatca ctgtcatgcc gctcacagcg
     1201 cgccatcgcc gtcgctgggc gtgaaagccc caaaaaagcc cgtcatcacg agctctctga
     1261 cggagaaatg cggacacgat ttcggtgaag actcgacgag agcgcgcgtc catcaccacg
     1321 aacacggagc gtgttgcgct cccacaaagg gtgaatgaaa tgaaacgccg tctacttgtc
     1381 ccgattgtca cggccggaat ggcgctcgcc tccggtgttg cctctgcaca aaccaacgct
     1441 cacacgctgg agtacttccg caactacggg ctgctgctcg aagtcagtgc ggacccgcgc
     1501 gatggctcgg acttcgtaca aacgaatccg acgcgcgccg gcaagctcga gctcgtcgcg
     1561 ctcaacgaca gtgttcgcct gcgcggcatg accgtacagt tctcggatgg ccgcacgttc
     1621 tcgcaaccgc tgcggacgat cggcgtcggc gagcgcgtga tggtcgacct gcccgcgaac
     1681 tgcggcctga tccagagcgt gcgtctcgat tacggtcgcc agatcgtcga tcgcacgccg
     1741 gcgcgcctgc agatcattcc gcacgacgag cagcggttca cgcagccgac gtatccgcgc
     1801 aacgatcggt ggagccggta ccagcagccg tcgcatcgga cctactcctc gcggccgacg
     1861 taccaacagc cgtcctacgt cgtgcgtccg gcgccccagc cgtcgtggtc ggcgttgggc
     1921 accatccagg gcagcttccg gttctagggc gcggaaatgg gacccctttt cgtaacctcg
     1981 tgagattggc tgtgactttg ctggtcgaac caatcgtaga ccgcgggtag ctcgctgtga
     2041 tagcgcagtg ggcatgcagc gagcgctcgc ggtcctacta ttcgtcgttc ccgtcgccgc
     2101 ctgtggtggc tcgagtgagt gcgatccgaa caccgagtcg tgcacgtacg aagcggacgt
     2161 cagcacgttg accgtcgccg cgggtgccga agacgaagac acctgccaga gctggacgct
     2221 caacaatccg accgagctct gggtcaacgc gatcacgcag acgaataacg gtggctatca
     2281 ccatgcgaac tggttcttcg tccccgacga tcagttcaac ctgcccgacg gcacgtggtc
     2341 gtgcgagcag aatcaattcg gcgagctgat cgccgcgctg caaggtggct atctgttcgc
     2401 gctcagcacg cagtcggcgg ccgagacgca gacgctgccg cacaactcgg cgatccgcat
     2461 tccgccgtac agccgcctga tcggttcgag ccatttgctc aacacgagtg acgaggacgt
     2521 cacgaccacg atgcacctcg cgatcacgac ggtgccgccg tccgaggtga aggcgaagct
     2581 cgcgccggcg cgcatgtcct atcacgcact cacgctcgag ccgcagacca agagcgcgtt
     2641 cacgatggag tgcgatctgc agaaggccta cgacgacgtc tggcacaagc cgctcgtcta
     2701 cacgctctac tacgtgctcg tgcactacca ctcgctcggc atctatcagc agctcgagat
     2761 catgggtggc ccgcgcgacg gcgaagtgat catgcaccac gacgggttcg gcgataacgc
     2821 cggccacccg atcgagccgc cactcgacct cgccgcagca ggtgcgacgg gcctgcgcct
     2881 cacgtgtggc ttcaacaatc cgcgcaccgc gcaggtcggc tggggcatcg gcgatcaaga
     2941 gatgtgcgtg atggcgctgc aagcgacgac gaatatcggc tgggatgccg acgtcacgca
     3001 gggcgcgctc accggcacct cggcgacggg cgagatgctg cacagcggtg cgtgctcggt
     3061 cgtgtcgttc ccgtgggact tcgacaagcc gggcggccaa ggtcactgat gcgcgttgcc
     3121 gtcgtcggcg gcggaatctt cggcgtcacc gcagctctcg cgcttcgcgc gcgcaagctc
     3181 gacgtccgac tctacgactc cgagctgccg catccgctcg ccgaatcgac ggacatctcg
     3241 aaggtcgtgc gcatggacta cggcgccgac gccgattaca cgcagctcgg cgagcaagcg
     3301 ctcgcaggtt ggcgccagtg gaacgcgcga tgggcgcgac cgctgttcca cgagaccggc
     3361 gtgaccttcc tctcgcgcga accgatgcag cccggtggct tcgaatacga gagctacacc
     3421 ctgctccgtc gccgcggtca tgcgctcgag cgtctcgatg cggacgcgat cgccgatcgc
     3481 cttccggcgt ggcggcgcgg cgcgtgcttc gatggttact tcaacccgca gggcggctgg
     3541 gccgagtcgg gcgccgtcgt cgccgcgctc gcggccgaag cagagaccgc gggtgtcgtg
     3601 atcgagcgcg tgcgcctcgc atcgctcgat gcactcgatg ccgagctcgt gatcgtgtgc
     3661 gccggctcgt gggtaccgct gctcgtgccc gcactcgcac ccgtgctgcg cgcggtcggt
     3721 caaccggtgt ttcatctccg tccggcggat ccgtcgctgt tcgaagcggc gcgctttccg
     3781 gtgttcggcg ccgacatctc gcgcaccggc tactacggct ttccggtcaa tcgcgatggc
     3841 atcgtgaaga tcgcgaacca cggcgtcggc ctcacgatcg caccgggcga tacgcggcct
     3901 gacgtcagcg acgcgcacga agcggcacta cgcgagatgc tgcgcgatac gtttcccgcg
     3961 ctcgtcgatg cgccgatcgt cggccgccgg ctctgcgtgt actgcgacac cgttgacgag
     4021 aacttctgga tcgcgcgcga tccggcccga cccaacgtcg tcgtcgcgac cggtggctcg
     4081 ggccacgcgt tcaaattcgc gccggtgctc ggcgatttga tcgcagcgat cgcgctcggc
     4141 gaaccgcatc tgctcgcgca caagtttcgc tggcgccccg agctcgctgc ccacggcggc
     4201 gaagctgcgc gtcatcacgg ctagccgatg cagccctcgg aaaactgagg gccggccacg
     4261 cgatctagcc gggttgcacg cggcgcgagt tttgctgcac gcttggtgat gatgcgcgcg
     4321 atgctaactg cgatcacgct cgtgatcgcc ggctgtggcg gtgacgacgg cgccctcccc
     4381 ggtgagccga tcggtgcaac gtgcgagatg aacatcgact gccagaccgg cgtgtgctgg
     4441 gactttcatg accacgacat gctgtgcgcc ggcaaggtgt gctcgacggc gtgcacgtcc
     4501 gacgccgaat gcgtcgacgc cgcgagcaag cacggcgcgt tcgggccgag caacgcacac
     4561 tgcggtagcg acggcctgtg cgatctggtc ggcaccgggc tcggcacctt tgcatgtcag
     4621 tgacgctatt cgggcgggcg acgggcaacg cgacgccgaa acgctagcgc cgattcaccc
     4681 gtccaccgcc ggaatgcgcg gctgaacgcg ctcagctcgg agtagccaag taagtacgct
     4741 gcgtccgtca acgacatgcc gcgatccgcg aggtagtcgc gcgcaagcga cagacgcgta
     4801 tcagcgacga gctgtttgaa cgacgtgcca cgatcctgca gccggcgctg aagtgtccgc
     4861 tcgctcgtac cgagctcggt tgcgacgtgc tcgatcgaaa cgtcgccggt acgcagcgac
     4921 gacgccacga tgctccgcgc ccgatcatgg acgtcgcgat cgagatagtg cggaaggccc
     4981 gcgtttgcgt gcacgaccaa gatcgggaac aggccagcgt cggcacggcg catcggtcgc
     5041 cggagctcgt cgggctcgac gatgatggcg tcctcggctt gaccgtacag cagctcgcaa
     5101 ccgaagaagt cacgctgcgt cgagctgttc tttggcttct cgtgcttgaa gtggatcgcg
     5161 cgcggcgccc accgcgggtc tccggtgccg tcgcgaatcg cgcgtacgta gaccgtaaac
     5221 accatctcgg tgttttgcgc gtgcctgacc accgtagggt cgtgcatcgc gtaacggaca
     5281 aacgcgtcct gcccttcgac cacgagctgc gtgcctgcac ccgtcgaatg caacgcgaag
     5341 tatcggcagg cgttcgcgag tgcagccccg accgtcggtg agttgagcac cacgtacgct
     5401 gcaacgccgt aatcggccca cggcagctgc gcagcgaatg tcatggtgag cgcggcgttg
     5461 ttcgttttca tcgccgccgt atcgagtagc tccgcggtgc gcttcacact gatccagccg
     5521 tcgggctttg caacctgtgc acttcggatg gctgcctcgc gcagcagcgc acgtgtcgac
     5581 aactcgtgat agcgccggat gtactcgacg aacctgcgaa cggtactcgc gcgcaatagt
     5641 cccgcccctg gcgcgggtcg tggctcgctc gtaacgtcaa tactcaagtc ggtggacacg
     5701 gtgaaagagg ctcgatgctg tagatccgct aacgatctca ggtgatgcag tcgagcacta
     5761 gtgatctttc gatcacgttc gccgcccctg tgtagcgcct gtggcgacgg caacaagaac
     5821 aacgtgcttc ggtaggagca gatgtggcac aggccgacgc gcccccacca ccaacccggg
     5881 atcatcctat tcccgacggc tgcacgccgt gcgcgctacg tgaaatgacg aacagacgcg
     5941 agaacacgct gcaactcacc ccgcgcgtcg ggatgcgcga ggctgatcat cgcctcgcct
     6001 cgctcgcgca acgtcagccc gtgcaggttg cgcgcgccgt actcggtcac gacccagtgc
     6061 acgtgaccgc gcgtcgtcac cacgccggcg cccggcttga gctcggcaac gatgcgcgac
     6121 acgctaccgc gcgcggcagt cgacggcatc gcgatgatcg gcttgccacc tcgtgacaat
     6181 gcagcgccgc gcaagaagtc catctggccg ccgatgccgg agtagatgcg atgaccgatc
     6241 gaatcggcgc acacctgccc gctcaaatcg atctcgagtg ccgagttgat cgcggtcatc
     6301 ttctcgttct tgcgaatcag cgcggtgtcg ttcgtgcgat cgcagccatg aaactcgatc
     6361 gccgcgttgt catcgacgaa gtcgtagagc gcacgcgtgc cattgacgaa gctcgtgacg
     6421 ctacgattcg ggtgcaccgc tttgtgccgg ttcgtgatca cgccgccttc gatgagcgga
     6481 atcatgccgt ccgaaaacat ctccgtatgc acgccgaggt cgacgcgatt gccaaggcgc
     6541 gcgagcaccg cttccggaat cgcgccgatg ccggtctgca gcgtcgcacc gtcttcgacc
     6601 agctgcgcga cgagctcacc gatgcgtgtc tcgacggccg tcggcgccgc cgcggcgtgc
     6661 tcgggcaacg cgcggttcgt acgcacgaac gcgtgcacgt ggtcgatgtg gacgctggtg
     6721 ttgccgtgcg tgcgcggcat ccgatcattg atctcggcaa gcacgacacc cgcgcaatcg
     6781 acggcggcgc gcgccgcgtc caccgacgtg ccgagcgagc aatagccatg acgatccggc
     6841 ggcgacagct ggacgagcgc gacatcgatc ggcaccgcgc ccgacgagaa caacgccgga
     6901 atgtccgaca agaacacggg cacgaagtca gcgcgccccg cctctaccgc gccccgcgca
     6961 ttcgcaccga cgaagaacga gatcgatcgc aagcgctccg tcacgctcgg cgccacgaag
     7021 ctgtccgtgc cctgcgtgtg caggtgatag agccggacgt cttcaacgtt agtccgcgcc
     7081 gcaagcgcat cgagcagtgg gatcggtgtc gccgctgcgc cgtgcacgaa cacgcgtgcc
     7141 cccgatggaa cgcgcgccgc aacgtcggcg gccgattcgc cgcgcgacga ccaggaacta
     7201 tccaggctca tgaccgcgca tcttgcccga tgcgtcgcca ag
//
LOCUS       DASUFX010000610         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1904163, whole genome shotgun sequence.
ACCESSION   DASUFX010000610 DASUFX010000000
VERSION     DASUFX010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904163"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1093
                     /locus_tag="VFV99_24505"
     CDS             110..1093
                     /locus_tag="VFV99_24505"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5062566.1"
                     /translation="MYDLPGAYINEILELAGRWNVRPAEILKGLPLTTAQLADPTTRV
                     PMRVVEAIVARAHQLTHEPALAIHVGTAMRLSSHGFLGFAAMTASNVQEALDLAVRFA
                     QTRTSAIGLAFYVEGDTACLVIDEKTDLGNLREFAVLALVIGIWQLGRALTGKVLDGV
                     AECTFPAPDWVARLPIGDRLRFNCPNHRLVFPSSTLAIPLITADPVATQLAREQCERE
                     LAILVDAGLPARVRAAITAKGDAPSLTVIAKELRMSSRTLKRKLADHGTTFSAIRDDM
                     RRQRALLLLDNRALSIGEIAMKLGYSELPNFTRAFRKWTGVTPLAYRERQR"
     gene            1093..1614
                     /locus_tag="VFV99_24510"
     CDS             1093..1614
                     /locus_tag="VFV99_24510"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5062567.1"
                     /translation="MYFAIRVAVGSAIMLAFYFTRGFGSYLQTFVMAFAASLAAQGVA
                     LGVKMIVGQQEKSKRAIALLGACVIAFAAAMVFVVIDKQTVMKKTAIHGVLDMSTAMR
                     NGETLEFTIDDRGKKMRVRYQGPLPDNARDRMEIAARGQWKGDVFVANELSTKCPTTY
                     NTEKGPVPASQFH"
     gene            complement(1674..>2418)
                     /locus_tag="VFV99_24515"
     CDS             complement(1674..>2418)
                     /locus_tag="VFV99_24515"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5062568.1"
                     /translation="KHTIYIENQYFSSYEIATALECRMRQVGPPLEIVMVMPKKSAGL
                     KERISIGVYQARILDRLSRLAADTGHRFGVYYQAAPHHDGKEVPVFIHAKVVAVDDRF
                     LLISSANLTNRSMGFDTELGVAWESPVPNESLRGARLELLAEHCGMPYDEAAARFASA
                     EGLVGKLDALARDKTHLLRIHERNVDEHPGWLLRRFLPRRTPLDPANPQQFREGLPEP
                     VVWLDRLIREPAVVLFGDRIPWLRKRPTQ"
BASE COUNT          424 a          785 c          774 g          435 t
ORIGIN      
        1 cgatattgaa atcgacatca cgacgtggaa tctgcatacg ctcagtctgg agtcgaccag
       61 caaacacccc taggtcactg cgtgccgagg ggttatcatt tggggccacg tgtacgacct
      121 gccgggcgca tacatcaacg agatcctcga gctggcaggc cgttggaacg tccgcccggc
      181 cgagattctc aaggggttgc cgctgactac ggcgcagctc gcagacccga ccacgcgtgt
      241 cccgatgcgc gtcgtcgagg cgatcgtcgc gcgcgcgcat cagctcacgc acgagcctgc
      301 gctcgcgatc cacgtcggca ctgcgatgcg tctctcctcg cacggcttcc ttggtttcgc
      361 ggcgatgacc gcgagcaacg tgcaagaggc actcgatctc gcggtgcgct tcgcgcagac
      421 gcgcacgtcg gcgatcggtc tcgcgttcta tgtcgaaggc gacaccgcgt gtctcgtgat
      481 cgacgagaag accgatctcg gcaacctgcg cgagttcgcc gtgctcgcgc tcgtgatcgg
      541 catctggcag ctcggtcgcg cactcaccgg caaagttctc gatggcgtcg ccgagtgcac
      601 gtttcccgcg cccgactggg tggcgcgcct accgatcggc gatcgcttgc gcttcaactg
      661 tccgaaccac cgcctcgtgt tcccatcgag cacgctcgcg attccgctca tcaccgccga
      721 tccagtcgcg acccagctcg cacgcgaaca gtgcgaacgc gagctcgcca tcctcgtcga
      781 cgccggcctg ccagcccgcg tgcgcgccgc gatcaccgcg aagggcgatg ctccgtcgct
      841 caccgtgatc gcgaaggagc tgcggatgtc atcgcgcacg ctcaagcgca agctcgctga
      901 tcacggcacc acgttctcgg cgatccgcga cgacatgcgc cgccagcgcg cgctgctctt
      961 gctcgacaat cgcgcgctct cgatcggcga gatcgcgatg aagctcggct acagcgagct
     1021 gccaaacttc acgcgtgcgt tccgcaagtg gaccggcgtg acaccactcg cgtaccggga
     1081 aaggcaacgc tgatgtactt cgcaattcgc gtggccgtcg gatccgcgat catgctcgcg
     1141 ttctacttca cgcgcggctt cggttcgtac cttcagacgt tcgtgatggc gttcgccgcg
     1201 tcacttgccg cgcagggcgt cgcgctcggc gtcaagatga tcgtcggtca gcaggagaag
     1261 tcgaagcgcg cgatcgcact gctcggcgcg tgcgtgattg cgtttgccgc ggcaatggtg
     1321 ttcgtcgtca tcgacaagca gaccgtgatg aagaagaccg cgatccacgg cgtgctcgac
     1381 atgagcaccg cgatgcggaa cggcgagacg ctcgaattca cgatcgacga tcgcggcaag
     1441 aagatgcgtg tgcgctatca ggggccgctt cctgacaacg cgcgggatcg catggagatt
     1501 gccgcgcgcg ggcagtggaa gggcgacgtg ttcgtcgcga acgagctgtc gacgaagtgc
     1561 ccgacgacgt acaacaccga gaagggcccg gtgcccgcgt cgcagttcca ctaaatgggg
     1621 tcagaccggg cagacccctt tattgccgat ttggcaaatg gggtcagacg cctttattga
     1681 gtcggacgct tgcgtagcca cgggatccga tcgccgaaga gcaccacggc cggctcgcgg
     1741 atcaaacgat cgagccacac gacgggctcc ggcaagcctt cgcggaactg ctgcgggttc
     1801 gccggatcga gcggcgtgcg tcgcggcagg aagcggcgca acagccagcc cggatgttca
     1861 tcgacgttgc gctcgtgaat gcggagcaag tgcgtcttgt cccgcgcgag cgcgtcgagc
     1921 ttgccgacga gaccttccgc tgacgcgaac cgcgctgcgg cttcgtcgta cggcatgccg
     1981 cagtgctcgg cgagcaattc gagacgcgcg ccgcgcagcg actcgttcgg gaccggcgat
     2041 tcccaggcga cgccgagctc ggtatcgaag cccatactgc gattcgtcag gttcgccgag
     2101 ctgatgagca ggaaccgatc gtcgacggcg acgaccttcg cgtggatgaa cacgggcact
     2161 tctttgccgt cgtggtgtgg cgcagcttgg taatagacgc cgaagcgatg gccggtgtcc
     2221 gcggcaagtc gcgataggcg gtcgaggatt cgcgcctgat agacgccgat cgagattcgc
     2281 tccttgagac cggcgctctt cttcggcatc accatcacga tctcgagcgg tggtccgact
     2341 tgccgcatcc ggcactcgag cgccgtcgcg atctcgtagc tcgagaagta ctggttctcg
     2401 atgtagatgg tgtgcttc
//
LOCUS       DASUFX010000611         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_376688,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000611 DASUFX010000000
VERSION     DASUFX010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376688"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..668)
                     /locus_tag="VFV99_24520"
     CDS             complement(198..668)
                     /locus_tag="VFV99_24520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062569.1"
                     /translation="MAETKRFKFKPRFRGLAFGAVGLGSALGGVSVIALGAAMLPLAT
                     GFAGVALGAAYLLSPTWKLEVVVDDEALEVRSARATKFRLPWSEVKRVVASQDTVTMF
                     IDGGSPEKSLLVPGDGAPAPYDLEDKPGLYGAVIAHVPADKIDKVARLDTVAKN"
     gene            complement(677..1108)
                     /locus_tag="VFV99_24525"
     CDS             complement(677..1108)
                     /locus_tag="VFV99_24525"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5062570.1"
                     /translation="MSAEARRAPRLVINAPAVVESIGQGPMQLHPNLARVFRRVDADR
                     SSLGSKFPAVVRDLSTNGAFVSGAPLPLLSRVAIKFDLKGVGMIDAIGWVLWQRMDDC
                     EVPSAQGSSTSLPKGFGVLFEAIPMETRSAIANLVNSFSRG"
     gene            complement(1117..3150)
                     /gene="metG"
                     /locus_tag="VFV99_24530"
     CDS             complement(1117..3150)
                     /gene="metG"
                     /locus_tag="VFV99_24530"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5062571.1"
                     /translation="MSTFYITTPIYYVNDVPHLGHAYTTIVADTLARFHRMRGDDTRF
                     LTGTDEHGQKVEETAKKKGMTPQQLVDAVAPRFDETWQTLGIKDYRFIRTTSEKHKKV
                     VASLWREIRKHTPDDLYLASYQGWYCVGCEAFYTESQLLKDGDTWKCAVHKTPVNWID
                     KERSWFFKLSKYAEPLLRHIEANPNFIRPEQYRNEIVSFLKSGVRDLSVSRTSFAWGI
                     PVPEADDQGDSHVIYVWMDALTNYLSDLCPPSGEISGPDVAKYWPQAIHVIGKDILRF
                     HAVYWPAFLMAAKLPLPKCIFAHGWWTVRGEKISKSMPATRIDPLKLSDALAVGPLGR
                     PIGIDAMRYYLLREVPLGLDGDFTFESLFGRFNAELANDLGNLVNRALSLMARSPLVP
                     AREESFGGVPHHAGLVATARQAIESATAEYENMAPSRALEAIWKLVREANRYVDTCQP
                     WALAKDPAKGMELAHVFANLANAIGVIGGLVAPVLPTTGRLLREWVGIDKGALDRWPT
                     ADDMHRQSMTVTKEIKPLFPRLDDATQAKILDTLVPPEAKGTAAPAEAPAAKAAATTS
                     GPPAQIVYDDFAKLDLRVGKVLQAALVPKKDKLIHLQVDLGEAKPRSIVAGIALSYKP
                     EELVGKRVIVVANLAPRKMAGLVSEGMILAAGESDSLSLSAIDRDAPPGTRVS"
     gene            complement(3147..>3843)
                     /gene="ricT"
                     /locus_tag="VFV99_24535"
     CDS             complement(3147..>3843)
                     /gene="ricT"
                     /locus_tag="VFV99_24535"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX5062572.1"
                     /translation="NLRRVIRRANDMDLRGDRDGEGDRTAALRIAKDVAAQLRLPLKV
                     FRVEKTGGKINVYYTSDERLDLRDFVQKVGQATNARLELRQLGVRDEAKAVGGIGSCG
                     LTLCCTTWLPEFVPVSIKMAKDQGLVLSPTKVSGQCGRLKCCLVYEQAGYAELRKGLP
                     KLGKRVISQRGEGRVVEVDVLRQRVRVSYGHGDTEVLPATEVKPMFPSGNQPREAEPP
                     DSDEHDETGLPDS"
BASE COUNT          639 a         1303 c         1242 g          659 t
ORIGIN      
        1 ccaccgcctc ggtcacatcg ccgagcgcat gagcagcggc agcacgtgcc ttcggactgc
       61 cgctcgcgag atcgcgcagc gcggcttcga aggtgatcgt cgggctgggg aacagaaacg
      121 acacggcgca accctagcag atgtagcggc cgtggcccct gccggacccg ctaggtgtgg
      181 accgtcccgc gcgcctctta gttcttcgcg accgtatcga gccgcgcgac cttgtcgatc
      241 ttgtccgcgg gcacgtgcgc gatcaccgca ccgtacagcc cgggcttgtc ctcgagatcg
      301 tacggcgcgg gtgcaccgtc gccggggacg agcaggctct tctcgggcga gccaccgtcg
      361 atgaacatcg tgaccgtatc ctgcgacgcg acgacgcgct tcacctcgct ccacggcagc
      421 cggaacttcg tcgcgcgcgc cgacctcacc tcgagtgctt cgtcgtcgac gacgacctcg
      481 agcttccacg ttggcgacaa cagatacgcc gcgcccagcg cgacgccggc gaacccggtc
      541 gcaagcggca gcatcgccgc gccgagcgcg atgaccgata cgccgccgag cgcgctgccg
      601 aggcccaccg cgccgaacgc gagcccgcga aagcgcggct tgaacttaaa ccgcttcgtc
      661 tcagccacgg gagagctcag ccacgggaga acgagttgac caagttcgcg atcgcgctgc
      721 gcgtctccat cgggatcgcc tcgaacagga cgccaaagcc tttcggcagc gacgtcgacg
      781 agccctgcgc gctcgggacc tcgcagtcat ccatgcgctg ccacagcacc cagccgatcg
      841 cgtcgatcat gccaacgccc ttcaagtcga acttgatcgc gacgcgcgac aagagcggca
      901 gcggcgcgcc cgacacgaac gcgccgttcg tcgagagatc tcgcacgacg gcaggaaact
      961 tcgaaccgag cgagctgcga tccgcatcga cgcggcgaaa cacgcgcgcg aggttgggat
     1021 gcaattgcat cgggccttgt ccgatcgact cgacaacggc cggagcgttg atcacgaggc
     1081 gaggtgcacg acgagcttcc gcggacatga cgcgagctag cttactcgtg ttcccggcgg
     1141 cgcgtcgcga tcgatggcgg aaagactgag gctgtcagac tcgccggccg cgaggatcat
     1201 cccctcggag accaggccgg ccatcttccg cggcgccaga ttcgcgacca cgatcacccg
     1261 tttaccgacg agctcctcgg gtttgtacga cagcgcgatg cccgcgacga tcgagcgcgg
     1321 cttggcctcg ccgagatcga cctggaggtg gatcagcttg tccttcttcg gcactagggc
     1381 ggcctgcagc accttgccga cgcgcagatc gagcttggcg aagtcgtcgt agacgatctg
     1441 cgccggcgga ccggaagtcg ttgcggctgc cttcgcagcc ggcgcctcgg caggcgcagc
     1501 cgttcccttc gcctcaggcg gcacgagcgt gtcgaggatc tttgcctggg tcgcgtcgtc
     1561 gagacgcggg aacagcggct tgatctcttt cgtcacggtc atcgactggc gatgcatgtc
     1621 gtcggcggtc ggccagcgat cgagcgcacc cttgtcgatg ccgacccact cgcgcaacag
     1681 gcggccggtc gtcggcagca ccggagcgac gagcccaccg atcacgccga tcgcattcgc
     1741 gaggttcgcg aacacgtgcg cgagctccat gcccttcgcc gggtccttcg cgagcgccca
     1801 cggctgacac gtgtcgacgt agcggttcgc ttcgcgcacc agtttccaga tcgcctcgag
     1861 cgcgcggctc ggcgccatgt tctcgtactc ggcggtcgca ctctcgatcg cctgtcgcgc
     1921 ggtcgcgacg agccctgcgt gatgcggaac accgccgaag ctctcctcgc gcgcgggcac
     1981 gagcgggctg cgcgccatca gcgacagcgc acggttgacg aggttgccga ggtcgttcgc
     2041 gagctcggcg ttgaaccggc cgaacagcga ctcgaacgtg aaatcaccgt cgaggccgag
     2101 cggcacctcg cggagtaggt agtagcgcat cgcatcgatg ccgatcggac ggccgagcgg
     2161 accgaccgcg agtgcatcag acagcttcag tggatcgatg cgcgtcgctg gcatcgactt
     2221 cgagattttc tcgccgcgca ccgtccacca gccgtgcgcg aagatgcact tcggcagcgg
     2281 cagcttcgcg gccatcagga acgcgggcca gtacacggcg tggaagcgca ggatgtcctt
     2341 gccgatgacg tggatcgcct gcggccagta cttcgcgacg tcgggaccgc tgatctcgcc
     2401 gctcggcggg cagaggtcgc tcaagtagtt cgtgagcgcg tccatccaca cgtagatcac
     2461 gtgcgagtcg ccctggtcat cggcttcggg aaccgggatg ccccacgcga agctcgtgcg
     2521 cgacaccgac agatcgcgga cgccgctctt caagaacgac acgatctcgt tgcgatactg
     2581 ctcgggccgg atgaagttcg ggttcgcctc gatgtggcgg agcagcggct cggcgtactt
     2641 cgacagcttg aagaaccagc tgcgctcctt gtcgatccag ttgaccggcg tcttgtgcac
     2701 ggcgcacttc cacgtgtcgc cgtccttgag caactggctc tcggtgtaga acgcctcgca
     2761 gccgacgcaa taccagccct gatacgacgc cagatagagg tcgtcgggag tgtgcttgcg
     2821 gatctcgcgc cacagcgacg cgacgacctt cttgtgcttc tcgctcgtcg tgcgaatgaa
     2881 gcggtagtcc ttgatgccga gcgtctgcca ggtctcgtcg aagcgcggtg cgacggcgtc
     2941 gaccagctgc tgcggcgtca tgcccttctt tttcgcggtc tcttcgacct tctggccgtg
     3001 ctcgtcggta ccggtcagaa accgcgtgtc gtcgccacgc atccggtgaa accgcgcgag
     3061 cgtgtcggcg acgatcgtcg tgtatgcgtg gccgaggtgc gggacgtcgt tgacgtaata
     3121 gatcggcgtc gtgatgtaga aggtgctcat gagtctggca ggccggtctc gtcgtgctcg
     3181 tcggaatccg gcggctcggc ttcgcgcggc tgatttccgc tcgggaacat cggcttaacc
     3241 tcggtcgctg gcaagacctc ggtgtcgccg tgtccataag atacccggac gcgctggcgg
     3301 agcacgtcga cctcgacgac ccgaccttca ccgcgctgag aaatgacgcg cttgccgagc
     3361 ttgggcaggc ctttgcgtag ctcggcgtag ccggcctgct cgtagacaag acagcacttg
     3421 agccggccac attgccccga gaccttcgtc ggcgacagca cgaggccctg atccttggcc
     3481 atcttgatcg acaccggcac gaactcgggc agccacgtcg tgcagcacag cgtgagtccg
     3541 cacgatccga tgccaccgac ggccttcgct tcgtcgcgca cgccgagctg gcgcagctcg
     3601 agtcgcgcgt tcgtcgcttg accgaccttc tgcacgaagt cgcgcagatc gaggcgctcg
     3661 tcgctcgtgt agtagacgtt gatcttgccg ccggtcttct cgacgcggaa caccttgagc
     3721 ggcagccgca gctgcgcagc gacatccttc gcgatgcgca aggcggccgt gcgatcgccc
     3781 tcgccatcgc ggtcaccgcg caggtccatg tcgtttgcac gccggatcac acggcgcagg
     3841 ttg
//
LOCUS       DASUFX010000612         9460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_376841,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000612 DASUFX010000000
VERSION     DASUFX010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376841"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1787
                     /locus_tag="VFV99_24540"
     CDS             171..1787
                     /locus_tag="VFV99_24540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062573.1"
                     /translation="MRTTALLAVSALSAGLLSQVGCASFDADGDPEDSVFVDDSKADD
                     FFSTAAAEYILEGTSTVTLDAAMANASADEKAAAAKKLVGYKQIAIAWFLTQYLVDKE
                     PDEANASFGGFSGIAKGGEFQDLSITPRADGLTYDFTFRQLAAAGKNLMSQLPIHTVN
                     GKPAFDLEIGKPTNAEMAQLETNNEWYRSAPWTEWNPANVAADKKEAMQFAISRERES
                     TDAFFDLERLTADGKLDIDVYFGWDYHSDYHLKHSQSFFTWLKSEGFTAPAASWDAMT
                     KDTGAFTRTVKADGKTVTVEVRFYFGKPGTATDPDTDAGGKVLEDLARASLTKRDVVV
                     YSGHSGPFYGFALANWRKTTEGDLDDSEMRTIAMPADRYQVVVAEGCDTYQIGEAFRE
                     NPNKAGKNVDVITTMSFSNASTPATVQNVLSALLARDSSGKLRPQPVSTLLTKLDSQS
                     WGFHSMYGLHGIDDNPKLVPFAKTGNFGKACSANTDCGGPGNLCVSTTGGKKCTAACA
                     ADPGCGTGYACRTVASSSTSTIYGKACLKL"
     gene            1832..3307
                     /gene="glgC"
                     /locus_tag="VFV99_24545"
     CDS             1832..3307
                     /gene="glgC"
                     /locus_tag="VFV99_24545"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5062574.1"
                     /translation="MSLGGPGLSMKDTLVMIMAGGKGSRLGPLTIHRSKPGVPFAGRY
                     RIIDFVLSNFVNSGYRRIYVLTQYMSSSLIKHLSRNWRLSGFGEYIEVVPAQMRLGEF
                     WYRGTADAVYQNLNLVRDARAEHVAVFGGDHIYKMAVDQMEEQHRDRAADLTVAAFPV
                     PVSEASAFGIIDVDDRGRIVRFVEKPKNPPEMPGRPGWSLVSMGNYLFRREVLERALS
                     DGGNGAPRTDFGRDIIPKLIADGARVFVYDFAQNRIPGEPEGVGPYWRDVGTIESYFT
                     SNMELRARVPALDLYNRQWRIRSAQRDYPPARFVRAGDGHPASEIDDSLICEGSILAS
                     ATVTESVIGYDCFVHSRAQVKDSIVLSGCDIGREARLSRVLLDKNCKIEPGCVIGEDP
                     EADAKRFPFVTESGIVVLPKGTLVPARGPCVLANDVAELINNDPELADKLEPGTFVVS
                     MQSRHSYDSAGPRFKKFSGDADPADTRLSSQAINAELETRR"
     gene            3331..4752
                     /gene="glgA"
                     /locus_tag="VFV99_24550"
     CDS             3331..4752
                     /gene="glgA"
                     /locus_tag="VFV99_24550"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX5062575.1"
                     /translation="MRVLHVTSEAAPFAQSGGLADVLGGLPPALAHAGFDVAVLLPLY
                     RGTEAKIAAAGATLDAGTPITIELGPHTYAARLRTTRFRDVIFGFIDAPLLYDRAGLF
                     YGPTGNSDYGDNYIRFGALGKVAVDHGASLLGGAVDVLHAHDWQGSPAATYARTEGMS
                     AAIVTTIHNLAYRGVFPKHVMTDLGHPWSLFTIHNLEFYDQVSFLKGGMANADAVTTV
                     SPSYAKEILTPQFGEALDGFLQHDVKRLVGIANGIDTEEWDPATDRALPASYSVDALA
                     GKAVCRRALAHELGLTLADDEPLIAVIARMTGQKGLDLVKDAVPELHRLGAKLVVLGS
                     GEPDLEATFTWLANAFKDQIAVRIGFDIALSRRIYAGSDLFLMPSRFEPCGLGQLYAM
                     RYGSIPVVHAVGGLRDTVTDPDTGIQFSAATVPALLDGLERAMKLFRDPKQLAATRRA
                     AMLRDSSWTASAQQYAALYRSLR"
     gene            complement(5385..6380)
                     /locus_tag="VFV99_24555"
     CDS             complement(5385..6380)
                     /locus_tag="VFV99_24555"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062576.1"
                     /translation="MPRIAVLALVLLVACKGSKQAKGRDAGAGDGGVDPTEVAGKLDK
                     RCVGGDMEACRNLGVMYAEGVGVSPDPRRATALFAQACNGRNLSACNHFALALSEGMG
                     VDRNPQRAALVYQQACDGGFTLACRNLGLMLRDGRGVPVDLQRADKLLDLACKGKVPF
                     ACTNAGDLDAMIAAKAPTPAPHWKSAIAHYKQGCESGDPTGCRQIGIAYLEARGLPRS
                     TAAASVWFERACVADDPIACRVLGAMLIDGVGVARDYKRGQELLTHACDRKDDEACRL
                     MTIAANPAAGDGGVPNSLGDGGLPLTASDAGSDSAAGTPVLRLDGGSFDAPSKTH"
     gene            complement(6395..7924)
                     /locus_tag="VFV99_24560"
     CDS             complement(6395..7924)
                     /locus_tag="VFV99_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062577.1"
                     /translation="MPELPRMRMLTQWGLRHGWLVAIAVAYLYAFPYFPRIHSANELP
                     RVYLVKAICDDGTFAIDKGVQRWGSTADVSPSDGHQYSNKAPGSSFLVVPVYALVSWI
                     GGEPSLATSMWLCRVVGGVIPALLFLWLLWGFLARYTPEPAVRRLVVLAYALGSLAMT
                     YSMLYYSHQLAAICIASSWILAVDVVEKKRSMRWMAAVGFFAGAALLVDYQAAFAGVP
                     IAVYLIVKLWAWPKRDLATALAIAAAAAAVPVAALLWYHKVCFGSPWRTGYDATQTFA
                     YLHKQGFLGISKLRKEAFWGSFFAPDNGLFVLSPWLLFALPGAVLLWRRGDRATTLVC
                     SAVAIIFILFISSISFWRGGWGVGPRYVTAMLPFWLPLVVVAIDALRSRPLVLGALCG
                     TIVSAVIIYCASAWTFPYWPDSVRNPLYEVTLRMLTDNAVAPNVASAIGIQGLLGLVP
                     LMAGIAALVGWALYRVVGWRGLAAAIVVGIAIVTVFRLAPINLYGQAAYLNTVYPAVT
                     R"
     gene            7955..>9460
                     /locus_tag="VFV99_24565"
     CDS             7955..>9460
                     /locus_tag="VFV99_24565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5062578.1"
                     /translation="MYAPRYRSVRDAIRADISRAIAMATGGALLFAPIEYALTVYAYS
                     GSIEWASKLRLAALTLTLALWLWVVLVVVTAGTMVAARLLRGVFDPRRARDLGWFVPS
                     PPVDGVRPGVPILWATIATIGAVGTFVQRGAVWANLHYQEKELTAIVIAAIALLGFAV
                     AFPLRRLFGIAADVGAKALAPTLRQWNPLGRWRAAGVACAALISGTLATCWFLLPQSR
                     SVLPVRLVITTVVVAFGMGRYAELHDRVAHRPRRRSYALAIAGIALALNVATLWRFGA
                     DPETKYVAITASPALDKLIQLVRTANDLDRDGFGSLLGENDCAPLDKSIHPGATDRPD
                     DGIDQNCDGRDFSLRAAPVPTGPAVAVPDQFKHSDWNVLLITIDTVRYDHTSFGGYKD
                     GPKKRDTTPRLAELAKKATSFTFCNAPSAGTMASIPAILTSKFFHSGLALDENLPPGN
                     PPKIKPENTTLPEIMKRAGYKTGVVASHNYWNDWGLDQGVDDYDNTIGKNAD"
BASE COUNT         1692 a         3195 c         3022 g         1551 t
ORIGIN      
        1 ctgccccctc gacgccgcca acacccggct cgacgcaggc tcagtagcaa gtcggccagt
       61 tacgccagtc cgccagccgg ctagcggagg cgccaggcga ccgcaagccg ctgatcggac
      121 ttggcgattc ggcccgatgt ccgggcatgg ccgttgctcg gacgtccgcc atgcgaacga
      181 ccgccctgct tgccgtctcc gccctgtccg ctggcctgct gtcgcaggtc ggctgcgcct
      241 ccttcgatgc cgacggagac cccgaggaca gcgtgtttgt cgatgacagc aaggcggacg
      301 acttcttcag caccgctgct gccgagtaca tcctcgaagg cacatcgacc gtgacgctcg
      361 acgccgcgat ggcgaatgcg agcgcggacg agaaagcggc agccgcgaag aagcttgtgg
      421 gctacaagca gatcgcgatc gcgtggttcc tgacgcagta tctcgtcgac aaggagcccg
      481 acgaggcgaa cgcgtcgttc ggcggcttct ccggcatcgc gaagggcggc gagttccagg
      541 acctgtcgat cacgccgcgc gcggatggcc tgacctacga cttcacgttc cgccagctcg
      601 ccgcagccgg caagaacctc atgtcgcagc tgccgatcca caccgtgaac ggcaaaccgg
      661 cgttcgacct cgaaatcggc aagccgacga acgccgagat ggcgcagctc gagacgaaca
      721 acgagtggta tcgcagcgcg ccgtggacgg agtggaaccc ggcgaacgtc gcggcggaca
      781 agaaggaagc gatgcagttc gcgatctcgc gcgagcgcga gtcgaccgac gcgttcttcg
      841 acctcgagcg cctcaccgcg gacggcaagc tcgacatcga tgtctatttc ggctgggact
      901 accactcgga ctatcacctc aagcattcgc agtcgttctt cacgtggctg aagagcgaag
      961 gcttcacggc gccggctgcg agctgggacg cgatgacgaa ggacactggc gcgttcactc
     1021 gcacggtcaa agccgacggc aaaaccgtca ccgtcgaggt gcgcttctac ttcggcaagc
     1081 cgggcacggc gaccgatccg gataccgacg ccggcggcaa ggtgctcgag gatctcgcgc
     1141 gggcatcgct cactaaacgt gacgtggtcg tctactcggg ccactcgggt ccgttctacg
     1201 gcttcgcact cgcgaactgg cgcaagacga ccgaaggcga cctcgacgac agcgagatgc
     1261 gcacgatcgc gatgccggcc gaccgctatc aggtggtcgt tgccgagggt tgcgacacgt
     1321 atcagatcgg cgaggcattc cgcgagaacc cgaacaaggc cggcaagaac gtcgacgtga
     1381 tcacgacgat gtcgttctcg aacgcgtcga cgccggcgac cgtccagaac gtcctatcgg
     1441 cgctgctcgc gcgcgactcg agcggcaagc tccgcccaca gccggtctcg acgctgctca
     1501 ccaagctcga cagccaatcg tggggcttcc actcgatgta tggcctccac ggcatcgacg
     1561 acaatccgaa gctcgtcccg ttcgcgaaga ccggcaactt cggcaaggcg tgctccgcga
     1621 acacggattg cggcggaccg ggcaatttgt gcgtctcgac cacaggcggc aagaagtgca
     1681 cggcagcctg tgcggccgac cccgggtgcg ggaccggcta tgcgtgccgg actgtagcga
     1741 gcagctcgac gagcacgatt tacggtaagg cctgcctgaa gctgtaggtt cgagcgcgct
     1801 cccgcgctgg ttcggttccc gtcttgctac gatgagtctc ggaggcccgg gactctcgat
     1861 gaaagacact ctggtgatga tcatggcggg cggtaagggc tcccgcctcg ggccgttgac
     1921 gatccaccgc tcgaagccgg gcgttccgtt cgccggtcgc tatcggatca tcgacttcgt
     1981 tctctcgaac ttcgtcaact cgggctatcg ccgcatctac gtgctcacgc agtacatgtc
     2041 gtcgagcctg atcaagcatc tgtcgcgcaa ctggcggctc tcgggtttcg gcgagtacat
     2101 cgaagtcgtg ccggcgcaga tgcgcctcgg cgagttctgg tatcgcggca ccgccgacgc
     2161 tgtgtaccag aacctcaact tggtgcgcga cgcgcgcgcc gagcacgtcg cagtgttcgg
     2221 cggcgaccac atctacaaga tggcggtcga tcaaatggag gaacagcatc gcgatcgcgc
     2281 cgcggatctc accgtcgccg cgtttccagt gccagtcagt gaagcgagcg cgttcggcat
     2341 catcgatgtc gacgatcgcg gccgcatcgt gcggttcgtc gagaaaccga agaacccgcc
     2401 ggagatgccc gggcgcccgg gctggtcgct cgtctcgatg ggtaactacc tcttccgtcg
     2461 cgaagtgctc gagcgcgcgc tcagcgacgg cggcaacgga gcaccgcgca ccgacttcgg
     2521 gcgcgacatc atcccgaagc tgatcgccga tggcgcgcgc gtgttcgtct acgacttcgc
     2581 gcagaaccgc atccccggcg agcccgaagg cgtcggcccg tactggcgcg acgtcggtac
     2641 gatcgagtcg tacttcacgt cgaacatgga gctgcgtgca cgcgtgcccg cgctcgatct
     2701 gtacaaccgg cagtggcgta tccgcagtgc gcaacgcgac tacccacctg cccgcttcgt
     2761 tcgcgctggc gatggccacc cggcgtcaga gatcgacgac agcttgatct gcgaaggctc
     2821 gatcctcgcg tcggccaccg tgaccgaatc ggtgatcggc tacgactgct tcgtgcactc
     2881 gcgcgcgcag gtgaaggatt cgatcgtcct gtcgggttgc gacatcggcc gcgaagcgcg
     2941 acttagccgc gtgctgctcg acaagaactg caagatcgag ccgggctgcg tgatcggtga
     3001 ggatcccgag gcggacgcga agcggttccc gttcgtgacc gagtccggca tcgtcgtatt
     3061 gccgaagggc acgctcgtgc cggcgcgcgg cccgtgcgta ctcgcgaacg acgtcgccga
     3121 gctcatcaac aacgaccccg agctcgccga caagctcgag cccgggacgt tcgtcgtgtc
     3181 gatgcagagc cgtcacagct acgactcggc ggggccgcgc ttcaagaagt tctcgggcga
     3241 tgcagatccc gccgacacgc ggctcagcag ccaggcgatc aacgccgagc tcgagacgcg
     3301 tcgctgatcc ggtcggcgta ggatatcgac gtgcgagtac tccacgtcac gagcgaggcc
     3361 gcgccgttcg cgcagtctgg tggcctcgcc gacgtgctcg gcggcttgcc gcctgcgctc
     3421 gcgcacgccg gcttcgacgt cgccgtgctg ttgccgctgt atcgcggcac cgaagcgaag
     3481 atcgctgccg ccggcgcgac actcgatgcc ggcacgccga tcacgatcga gctcggtccg
     3541 cacacgtacg ctgcgcggct tcgcacgacc cgctttcgcg acgtcatctt cggcttcatc
     3601 gacgctccgc tcctctacga ccgcgccggt ctgttctacg gccccaccgg caacagcgac
     3661 tacggcgaca actacatccg cttcggcgcg ctcggcaaag tcgccgtcga ccacggcgca
     3721 tcattgctcg gcggcgccgt cgacgtgctt cacgcgcacg actggcaagg ctcgccggca
     3781 gcgacctatg cccgcaccga aggcatgagc gcggcaattg ttacgacgat tcacaacctc
     3841 gcctaccgcg gcgtcttccc caagcacgtg atgacggacc tcggtcatcc gtggtcgctg
     3901 ttcacgatcc acaacctcga gttctacgat caggtctcgt ttctcaaagg cggcatggcg
     3961 aacgcagacg ccgtgacgac agtgagcccg agttacgcga aggagattct gacgccgcag
     4021 ttcggcgaag cgctcgacgg atttttgcag cacgacgtga aacgactggt cggcatcgcg
     4081 aacggcatcg ataccgaaga atgggatcca gcaaccgatc gcgcgttgcc cgcgagctac
     4141 tcggtagacg cgcttgcggg caaagccgta tgccgccgtg cgctcgcgca cgagctcggc
     4201 ctgacactcg cggacgacga accgctgatc gcagtgatcg cgcgcatgac cggacagaag
     4261 ggcctcgatc tcgtcaaaga cgcggtgccg gagcttcacc ggctcggcgc gaagctcgtc
     4321 gtgctcggat ccggagagcc tgatctcgaa gcgacgttca cttggctcgc gaacgcgttc
     4381 aaagatcaga tcgccgtccg gatcggcttc gacatcgcgc tctcgcgcag aatctacgcg
     4441 ggcagcgatc tgttcctcat gccgtcgcgc ttcgagccat gcggactcgg ccagctgtat
     4501 gcgatgcgct acggatcaat tccggtcgtg catgcagtcg gtggtttgcg cgacaccgtg
     4561 accgatcccg acaccgggat ccaattttct gcagcgacgg tccctgcgtt gctcgacgga
     4621 ctcgagcgcg cgatgaaact gtttcgcgat ccgaagcagc ttgcggctac gcgccgagcc
     4681 gcgatgctgc gtgattcatc gtggaccgcg tctgcgcagc aatacgcagc gctctatcgc
     4741 tcactgcgtt gaacggtacg gcgcacactc ctttgaatgg agtgcagcga tttgctgcgc
     4801 cgtggtttgc tgcagtgtga tcattctgta gtcatggtta ccccgtacgc agcagccgtg
     4861 acggcgcttc tcgacgacga atcgtgctcg accgtccgct tcgcggacag atttggtccg
     4921 tactgcggac ggctcatcga atcctcgacg ggccccgtgc tgtccgcgcc tcggacccgg
     4981 tccgtccgca ggtcggacct caccgtccgc cagacggacc tgggcgtccg ctttgcggac
     5041 ctaacgcaca ctgccaagcc gccccatgtg cggcccggtt acactttcgg ccaggccagc
     5101 tggtccctca gcctgaaccc gaatagctgt gatcaggcct gtcaacaact acgtgcctgg
     5161 gtcgggttat tcacaaccca actcagacct aaagtcccga tttacaagga cccaccggga
     5221 tcgccactgc acctgtgaac aagttgtgat cccggtgttg gggtgggttc cgggagcgac
     5281 ggcgcttcga taacttacgc tatatgtcgc tcgatatact ccgcgcgcct acatggtggc
     5341 atagcccacg tactcccgtg cgggagtgag cggcaagagc acggtcaatg agtcttactc
     5401 ggtgcgtcaa atgaaccgcc atcgagccga agaacggggg ttccggcagc cgagtctgaa
     5461 cctgcatcgc ttgccgtgag cggcagtccg ccgtcaccca gtgaattggg aacgccgcca
     5521 tcaccggctg ccgggttcgc agcgatcgtc atcaagcgac acgcttcatc gtctttgcgg
     5581 tcacacgcgt gtgtcagcag ctcttggccg cgtttgtagt cgcgcgcaac gcccacgcca
     5641 tcgatcaaca tcgcaccgag cacgcggcag gcgatcggat cgtcggcgac gcacgcgcgc
     5701 tcgaaccata ccgaagcggc cgcagtcgat cgcggcagac cacgtgcttc gaggtaggcg
     5761 atcccgatct ggcggcagcc ggttgggtcg ccgctctcac agccttgctt gtagtgggcg
     5821 atcgcgcttt tccagtgcgg tgcaggagtg ggcgcttttg ccgcaatcat cgcgtcgaga
     5881 tcgcctgcgt tggtacacgc aaacgggacc tttcctttgc acgcgaggtc gagcagcttg
     5941 tccgcacgct gcagatcgac cggcaccccg cggccgtcac gcaacatgag cccgaggttg
     6001 cggcacgcga gcgtaaagcc gccgtcgcac gcttgctgat agaccagggc cgcgcgctgc
     6061 ggattgcgat cgacgcccat gccttcggag agcgcgagcg cgaagtgatt gcacgcggac
     6121 aggttcctgc cgttgcacgc ctgggcgaac agcgcggtcg cgcgtcgcgg atcgggcgag
     6181 acgcccacgc cctctgcgta catcacgccg aggttgcggc acgcttccat gtcgccgccc
     6241 acgcagcgct tgtcgagctt gcccgcgacc tcggtcggat cgacacctcc gtcgcccgcg
     6301 cccgcgtcgc gtcccttggc ctgcttgctg cccttgcagg cgacgagcag cacgagcgcg
     6361 aggacggcga tacgaggcat acgcgcgagt cgtatcaccg agtcaccgcc gggtacaccg
     6421 tgttgaggta cgcggcttgc ccgtacaggt taatcggcgc gagtcgaaac acggtgacga
     6481 tcgcgatacc gacgacgatg gcggcggcga gcccgcgcca gcccacgacg cgataaagcg
     6541 cccagccgac gagcgccgcg atgccggcca tcagcggcac gaggccgagc agtccctgaa
     6601 tcccgatcgc gctcgcgacg ttcggcgcga ccgcattgtc cgtgagcata cgcagcgtca
     6661 cctcgtacag tggatttcgt accgagtcag gccagtacgg gaacgtccat gcggaagcgc
     6721 aatagatgat gaccgcgctc acgatcgtgc cgcacaacgc tccgagcacg agcggccgcg
     6781 agcgcagcgc atcgatcgcg acgacgacga gcggcagcca gaacgggagc atcgcggtca
     6841 cgtagcgcgg gccgacaccc cagccgccgc gccagaacga gatcgacgag atgaacagga
     6901 tgaagatgat cgcgaccgcg ctgcacacga gcgtcgtcgc gcggtcaccg cgccgccaaa
     6961 ggagcaccgc gccgggcagc gcgaacaaca gccacggcga cagcacgaac aggccgttgt
     7021 cgggcgcgaa gaacgagccc cagaacgctt ccttgcgcag cttgctgatg ccgaggaagc
     7081 cctgcttgtg cagatacgcg aacgtctgcg tcgcgtcgta gccggtgcgc cacgggctgc
     7141 caaagcagac cttgtggtac cagagcagcg cggctaccgg caccgccgct gccgctgccg
     7201 cgatcgcgag cgcggtcgcg aggtcgcgct tcggccacgc ccacagcttc acgatcagat
     7261 agacggcgat cggcacgccg gcgaatgcgg cctggtagtc gacgagtagc gccgcaccgg
     7321 cgaagaagcc gaccgccgcc atccaccgca tgctgcgttt cttctcgacg acgtcgaccg
     7381 cgaggatcca cgagctcgcg atgcagatcg ccgcgagctg gtgcgagtaa tagagcatcg
     7441 agtacgtcat cgcgagcgag ccgagcgcgt acgcgagcac gacgagccgc cgcactgccg
     7501 gctctggcgt gtagcgcgcg agaaagcccc acaagagcca caagaacagc agcgccggga
     7561 tcacgccgcc gacgacgcgg catagccaca tcgaagtcgc gagcgacggc tctccaccga
     7621 tccagctgac cagcgcgtag accgggacga cgagaaacga cgatcccggc gccttgttcg
     7681 agtactgatg accatccgac ggcgagacgt cagcggtcga gccccagcgc tgaacgccct
     7741 tgtcgatggc gaacgtgccg tcgtcgcaaa ttgccttgac gaggtagacg cgtggcagct
     7801 cgttcgcgct gtggatccga gggaaatacg gaaacgcgta gaggtacgcc accgcgatcg
     7861 cgacgagcca gccatggcgt agcccccact gggtcagcat gcgcatgcgc ggcagctcgg
     7921 gcacgttcct gccgaacgtg ataccaccgg tttgatgtat gcgccgcgct atcgctcggt
     7981 tcgcgatgcg atccgtgcgg acatcagccg ggccatcgcg atggcgaccg gcggcgccct
     8041 cctatttgcc ccgatcgagt acgcgctgac ggtgtacgcc tactcgggct cgatcgagtg
     8101 ggcgagcaag ctgcggctcg ccgcactaac gctgacgctc gcgctgtggc tgtgggttgt
     8161 cctggtcgtc gttaccgcgg gcacgatggt agccgcgcgg cttctgcgcg gcgtattcga
     8221 tccccgccgc gcgcgcgacc tcggctggtt cgtcccttcc ccgccggtcg acggcgtgcg
     8281 gccgggcgtg ccgatcctgt gggccacgat cgcgacgatc ggcgcggtcg gcacattcgt
     8341 ccagcgcggt gcggtgtggg cgaacctcca ttaccaggag aaggagctga ccgcgatcgt
     8401 gatcgcggcg atcgcgctgc tcggattcgc cgtcgcgttt ccgctgcgcc ggctgttcgg
     8461 catcgcggcc gacgtcggcg cgaaggctct cgcaccaacg ctgcgccagt ggaatccgct
     8521 tggtcggtgg cgcgcggctg gcgtcgcatg tgcagcgctg atcagcggca cgctcgccac
     8581 atgctggttc ctgctgccgc agagccgctc ggtgctgccg gtccggctcg tgatcacgac
     8641 ggtcgtcgtc gcattcggca tgggccgcta cgccgagctt cacgatcgcg tcgcgcaccg
     8701 gccgcgccgt cgcagctacg cgctggcgat cgccggcatc gcgctcgcgc tcaacgtcgc
     8761 caccctctgg cgcttcggcg ccgacccgga gacgaagtac gtcgcgatca ccgcgtcgcc
     8821 ggcgctcgac aagttgatcc agctcgtgcg caccgcgaac gatctcgatc gcgacggatt
     8881 cggctcgctg ctcggcgaga acgactgcgc gccgctcgac aagtcgatcc accccggcgc
     8941 gaccgaccgc cctgacgacg gcatcgatca gaactgcgat ggccgcgact tctcattacg
     9001 cgcggcgccg gtaccgacgg gccctgcggt cgcggttccg gatcagttca agcacagcga
     9061 ctggaacgtc ctgctcatca cgatcgacac cgttcgctac gaccacacgt cgttcggtgg
     9121 ctacaaggac gggccgaaga agcgcgacac gacgccgcga ctcgccgagc tcgcgaagaa
     9181 ggcgacgtcg ttcacgttct gcaacgcgcc gagcgcgggc acgatggcat cgattcccgc
     9241 gatcctgacc tcgaagttct tccactcggg cctcgcgctc gacgagaact tgccgcccgg
     9301 gaatccgccg aagatcaaac ccgagaacac gacgctgccc gagatcatga agcgcgcggg
     9361 gtacaagacc ggcgtcgtcg cgtcgcacaa ctactggaac gactggggcc tcgatcaggg
     9421 tgtcgacgac tacgacaaca cgatcggcaa gaacgccgac
//
LOCUS       DASUFX010000613         7455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_376944,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000613 DASUFX010000000
VERSION     DASUFX010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376944"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFV99_24570"
     CDS             complement(<1..220)
                     /locus_tag="VFV99_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062579.1"
                     /translation="MSACYSPTVPAGAPCDLALNNCPAGQRCEMTASGAFCGAVPTLV
                     DGPTSNGDGPDGPRPDTAPGCFGVGLIHD"
     gene            405..1688
                     /locus_tag="VFV99_24575"
     CDS             405..1688
                     /locus_tag="VFV99_24575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5062580.1"
                     /translation="MFPRTGLWAHADFMRLWSAQAISAFGSRITRTALPIIAVQTLQQ
                     SETVVALLSSVYLAPGVLLALFAGGVVDRSRKRRILMAADIFRAATVASITLAWLFGA
                     LSMWHLVMVGALVGAASALFTIADVAYLPTLVDKRQLGEGNAKLEATEAVAEVTGPAS
                     AGVLIGALGAPLAVAIDAASYLWSALMLGRIRAAEPPPQPAPSAQSSTWQSRRDLREG
                     MRAIFGNQHVRPVVSALMVWSIAGGFFTALYTLFCLRTLGLSTLTFGVIIALGGIGSF
                     GGAIISRRLVTSIGLGRTMIVSAVISLAGGLLIPLARGSHATVLLLLGAHQLISDCFS
                     VAFVIQAVTLRQTVLPKYLLGRANAAVHVCTAGLVPIVALLAGLLAELTSIRTAVWVG
                     VSIGLVAPLFLLPLRNLRWMPESHSSTEIARASGG"
     gene            1729..2445
                     /locus_tag="VFV99_24580"
     CDS             1729..2445
                     /locus_tag="VFV99_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062581.1"
                     /translation="MSRLLITASLLFAFSATATASESHFAQPPAADRAELMPRAGDVA
                     VPPAGYRVAPRRVLDRTTVRAALAQARATNLAAFRVYQKRGVFPSNTFKPGKLNVWLD
                     ADGHFCAAATIIKMSGQDDLVTKVAEQNNFIRLADVKQGPLMDWILTSGLTQDEIAAI
                     QEPFRPVVDEPKLEPVKPILVDAKLRQAEDARLRAKYTAVDKMIVKNAKQSLDLATDR
                     LMKHPTLAWQLINSQRTIEG"
     gene            complement(2486..3874)
                     /locus_tag="VFV99_24585"
     CDS             complement(2486..3874)
                     /locus_tag="VFV99_24585"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5062582.1"
                     /translation="MKTLPSLADDAKRLCQSLLRIDTTNPPGNERAAAELITTALREV
                     DLEPQLLEAEPHRTNLVVRYRGTGAKPPLLLTAHLDVVEADPTKWRRPPFSGEEHDGC
                     LWGRGAIDMKNMAAMCTAILRRLAAKNVLLERDVIFAAVADEEAGCDLGSRFLVEQHK
                     HLVEAEYAIGESGGFSLHLGDSTFYPVQVAEKGFCWIKARVTGEPGHGSMPRHDSVVT
                     KLGHALTRLGEAHMPVHQTKYVTDFLDALRARQPALIQPLVKLVARPQLLARVARLIP
                     GVSIARSFSALLSNTAAATVVRAGAKTNVIPGVAEFEIDGRTLPGQSDEDLLRELRAV
                     LGDEVELTIMKSAPPVVTEPIASPVFDIIKRHVESREPDAQVVPYLIPGFTDAKYFTR
                     MGARWYGFSPVKIEKGSGIRFADMFHGHDERVPVAGLAWGVEVLDAVVREIAVEQTTS
                     ARSSSSTTARVS"
     gene            complement(4003..4734)
                     /locus_tag="VFV99_24590"
     CDS             complement(4003..4734)
                     /locus_tag="VFV99_24590"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX5062583.1"
                     /translation="MDEVVERDGGEIDGQPRVDAGMPPDASCGMTGTLRTVLLGARMN
                     GTGQASMIDTFLVDIKSGQNFGGAPMIGLCAACNASGYPWDMGNAVGLLRFELSAICP
                     GSTITGATLFIDTNDDNLGSGTVGVYVVRQAWDEGDGSAAGNLGVANWNQRQPGVNWN
                     NPGAGSPQSRDGTPFMVFAPTSTDQEYQLSVPIVVVQDWLNKPATNFGLALAIVNGSS
                     DVEFHSRESPEVARRPGLSVSFTLP"
     gene            complement(5090..5181)
                     /locus_tag="VFV99_24595"
     tRNA            complement(5090..5181)
                     /locus_tag="VFV99_24595"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5145..5147),aa:Ile,seq:gat)
     gene            complement(5584..5997)
                     /locus_tag="VFV99_24600"
     CDS             complement(5584..5997)
                     /locus_tag="VFV99_24600"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5062584.1"
                     /translation="MTISTRTLTRPLIAMLALFLLFLSMGGTWHLASAAPPAAIAAKP
                     ASASSAGDDDGERPIKKSAKDLTGKLNLNTATEEQLMMLPTVGPAKADRIVTWRKKNG
                     GFKRTADLRRVKGFGYKTFKRLEPFLDIKGDTTLK"
     gene            complement(6006..6317)
                     /locus_tag="VFV99_24605"
     CDS             complement(6006..6317)
                     /locus_tag="VFV99_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062585.1"
                     /translation="MFQQKRMFKVITPIDRRDGGKFWMRLGTAYVNKDNSINAYIDAI
                     PLVPSKDGQGVTLQLREFTDEELRERDEKKATYNARGTLSNQFTPPAQTGPAAVDAVP
                     F"
     gene            complement(6504..>7455)
                     /locus_tag="VFV99_24610"
     CDS             complement(6504..>7455)
                     /locus_tag="VFV99_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5062586.1"
                     /translation="IACGGSQRVPLAPPGDDKDEGAGELARAARKFETSQDDSVAGFN
                     DERGPPRYDIDNAYGGDMYGGDPYGGFGGDPYGGSGYANWRMPQWSYNVPNRVPRYSV
                     SIGLTGAIEGTVTWNGQLPAKVASACGLIDNPTLRVSKDKAVRGVIVYIEKVTIGRST
                     PYYSRPASVGGMLAKHNCTLAPAAQIVAPLPGSVAINGDTQRTKLRIGKVTQELQEGG
                     LVQVDVKAGVTKIESEDGKVAPAWVLGLETPYYAVTDDEGRFRLDELSPGTYEITFWQ
                     PPIATANRDGTWTSGAPIVVKRSVKVDAKTTHISVSLPGH"
BASE COUNT         1223 a         2499 c         2396 g         1337 t
ORIGIN      
        1 cgtcgtggat caagccgacc ccgaaacaac cgggcgcggt atcggggcgc ggaccatcgg
       61 gaccatctcc gttcgacgtc gggccatcga cgagcgttgg caccgcgccg cagaacgcac
      121 cgctcgcggt catctcgcag cgctgacctg caggacagtt gttgagcgcc agatcgcaag
      181 gcgcgccggc aggcacggtc ggcgaataac acgccgacac ggcgacgagc atacctgccg
      241 ccacgcgcat gcccataagg atatcggcgt tccctcacgg gatccatgac gcgctcttgg
      301 ggccgtcggg gtgtttctca caccgccact gaccactact tgcacgatct gatcacggct
      361 gtgaacagcg tgccgaactg gcgcattggg gcatcatcat tgcgatgttt ccgcgcaccg
      421 gcctgtgggc ccacgccgac ttcatgcggc tctggtctgc gcaggcaatc agtgccttcg
      481 gctcgcggat cacgcgcacg gcgctgccga tcatcgcggt ccagacgctg caacagtcgg
      541 agaccgttgt cgcgctgctg tcgtcggtct atctggcgcc aggggtattg ctcgcgctgt
      601 tcgcgggcgg cgtcgtcgat cgcagtcgca agcgccgcat cctgatggcg gcggacatct
      661 tccgcgcggc gaccgtcgcg tcgatcaccc tggcgtggct gttcggtgcg ctgtcgatgt
      721 ggcacctggt gatggtgggc gcgctggtcg gtgcggcatc cgcgttgttc acgatcgcgg
      781 acgtcgcata cctgccgacc ctcgtcgaca agcgccagct cggcgaaggc aacgcgaagc
      841 tcgaagccac cgaagcggtc gccgaggtca ccgggcccgc gagtgcgggc gtcttgatcg
      901 gcgcgctcgg tgcgccgctc gccgtcgcga tcgatgctgc gagctacttg tggtccgcgt
      961 tgatgctcgg ccgcattcgc gccgccgaac cgccaccaca gccggcgcca tcggcccagt
     1021 cgagcacgtg gcagtcgcgc cgcgatctgc gcgagggcat gcgagcgatc tttggaaatc
     1081 agcacgtgcg tcccgtcgtc agcgcgctga tggtgtggtc gatcgcgggc ggcttcttca
     1141 ccgcgctgta cacgctgttt tgcctccgca cgctcgggct ctccacgctc acgttcggcg
     1201 tgatcatcgc gctcggcggc atcggctcgt tcggcggcgc gatcatctcg cgccggctcg
     1261 tcacctcgat cggccttggc cgcacgatga tcgtgagcgc ggtgatcagc ctcgccggcg
     1321 gcctgttgat cccgctcgca cgaggctcgc acgcgacggt gttgctcttg ctcggcgcgc
     1381 atcaattgat cagcgactgc ttctcggtcg ccttcgtgat ccaggcggtc acattgcggc
     1441 agacggtact gccgaagtat ttgctcggtc gcgcgaacgc cgccgttcac gtgtgcaccg
     1501 ctggcctcgt gccgatcgtc gcgctgctcg ccgggttgct cgcggagctc acatcgattc
     1561 gcaccgcggt gtgggtcggt gtgtcgatcg ggctcgtcgc accgttattc ctgctgccac
     1621 tccgcaatct gcgctggatg cccgaatcgc actcttcgac ggaaatcgcg cgcgcatcgg
     1681 gcggttagcg acatagaccg actcgcgcgg cgacgcgttg atatcggcat gtcgagacta
     1741 ctcattactg cttcgcttct gttcgcgttc tctgccactg ccaccgcgtc cgagtcgcat
     1801 ttcgcacagc cgcctgccgc cgatcgcgcc gagctcatgc cgcgcgctgg cgatgtcgcg
     1861 gtcccgccgg ctggctatcg cgtcgcacca cgccgcgtgc tcgatcgcac gaccgtacgc
     1921 gccgcgctcg cgcaagcgcg tgcgacaaac ctcgcggcgt tccgcgtcta tcagaagcgc
     1981 ggcgtgttcc cgagcaatac gttcaagccg ggcaagctca atgtctggct cgatgcagac
     2041 ggtcacttct gtgcggccgc gacgatcatc aagatgtcgg gccaggacga tctcgtgacc
     2101 aaggtcgccg agcagaacaa cttcatccgc ctcgcggacg tcaagcaggg accgctgatg
     2161 gactggatcc tgacgtcggg cctcacgcag gacgagatcg ctgcgatcca ggagccgttc
     2221 cgcccggtcg tcgacgagcc gaagctcgag ccagtgaagc ccatcctcgt cgacgccaag
     2281 ctccgccagg cggaagacgc tcgcctgcgt gcgaagtaca ccgcggtcga caagatgatc
     2341 gtcaagaacg cgaagcagag cctcgacctc gcgaccgatc gcctgatgaa gcacccgacg
     2401 ctcgcgtggc agctgatcaa ctcgcagcgc accatcgaag gctgagcaat cacgccgcgc
     2461 gccctgcgcg ttggcaacag ccgatttacg aaacgcgcgc ggtcgtcgac gaggacgatc
     2521 gcgcgctcgt cgtttgttcg acggcgatct cgcgcaccac tgcatcgagc acctcgacgc
     2581 cccacgcgag ccccgccacc ggcacgcgct cgtcgtggcc gtggaacatg tcggcgaagc
     2641 gaatccccga gcccttctcg atcttcaccg gcgagaagcc gtaccaacgc gcacccatgc
     2701 gcgtgaagta cttcgcatcg gtgaagcccg gaatcaggta cggcactacc tgggcatcgg
     2761 gctcgcggct ctcgacgtgg cgcttgatga tgtcgaacac cggtgacgcg atcggctcgg
     2821 tcaccaccgg cggcgcgctc ttcatgatcg tgagctcgac ctcgtcgccg agcaccgcgc
     2881 gcagctcgcg cagtagatcc tcgtcacttt gacccggcag cgtccgcccg tcgatctcga
     2941 actccgcgac gcccgggatc acgttcgtct tcgcgccagc gcgcacgacg gtcgccgccg
     3001 ccgtgttgct gaggagcgct gaaaagctcc gcgcgatcga cacgccagga atgagccgcg
     3061 cgacacgcgc gagcaactgc ggccgcgcga ccagcttcac gagcggctgg atcaacgcgg
     3121 gctgccgcgc gcgcaacgca tccaagaagt cggtcacgta cttcgtctgg tgcacgggca
     3181 tgtgcgcttc gccgagccgc gtcagcgcat ggccgagctt cgtcaccacg ctgtcgtgac
     3241 gcggcatcga accgtggccg ggctcgcccg tcacgcgcgc cttgatccag cagaagcctt
     3301 tctcggcgac ctgcaccgga tagaatgtcg aatcgccgag gtgcagcgag aagccacccg
     3361 actcgccgat cgcgtactcc gcttcgacca ggtgcttgtg ctgctcgacg agaaaccgcg
     3421 agcccagatc gcaccccgcc tcttcatcgg caacggctgc gaagatcacg tcgcgctcga
     3481 gcagtacgtt cttcgcggcg agccgccgca agatcgcggt gcacatcgcc gccatgttct
     3541 tcatgtcgat cgcgccgcgt ccccacaaac agccgtcgtg ctcttcgccc gagaacggcg
     3601 gccgccgcca cttcgtcgga tccgcctcga cgacgtcgag atgcgcggtc aacagcaacg
     3661 gcggcttcgc gccggtgccg cgatatcgca ccaccaggtt cgtgcgatgc ggctccgctt
     3721 cgagcaactg cggctcgaga tcgacctcgc gcaacgcggt cgtgatcagc tcggcggccg
     3781 cgcgctcgtt gccgggcgga ttcgtcgtgt cgatcctgag cagcgattga cacaggcgct
     3841 tcgcgtcgtc ggccaagctc ggcaacgtct tcacggggcg actgtaaaga gcagcgcggc
     3901 gccgttctag ccgcctcgaa cggaaatcga cgagtccggc agtagtcgcg gcgagcaaat
     3961 cgacgagctt ggccgtggta gcggactgtg acgcagtttc gcctacggca acgtgaagct
     4021 aacgctgagc ccgggacgtc gcgccacctc tggactctcg cgcgagtgaa actcgacgtc
     4081 ggagctgccg ttgacgatcg cgagcgcgag cccgaagttc gttgcgggct tgttcaacca
     4141 gtcttgcacc accacgatcg gaaccgacag ctgatattcc tggtcggtag aggttggcgc
     4201 gaacaccatg aatggcgtgc catctcgcga ctgaggcgac ccggcgccgg ggttgttcca
     4261 gttcacgccg ggctggcgtt ggttccagtt cgcgactccg aggttgccgg ccgcgctgcc
     4321 gtcgccttca tcccaggctt gccgcacgac gtacacaccg acggtcccgc tgcccaggtt
     4381 gtcgtcgttc gtatcgatga acagcgtcgc acccgtgatc gtcgagccgg ggcagatcgc
     4441 cgacagctcg aagcgcaaca ggcctaccgc gttccccatg tcccagggat aaccagacgc
     4501 gttgcacgct gcacacaacc cgatcatcgg cgcgccgccg aaattctggc cactcttgat
     4561 gtcgacgagg aacgtgtcga tcatcgatgc ctgtccggtg ccattcatgc gggcgccgag
     4621 cagcaccgtc cttagcgtgc cggtcattcc gcacgacgca tcaggcggca tgccggcgtc
     4681 gacgcgcggc tggccatcga tctcgccgcc gtctcgctct actacttcgt ccacgtgttg
     4741 caggctgaag agcttgtcgc agccagtggt cgcgcacgcg acccccagtg ctatcgccat
     4801 tccccgcacc gatcgatcag accccatgat cgaacgggca gtcaatcgca cacgccacgc
     4861 gtggacgtgc tcacaaggtg gtcaacgctg ccctcgcggt tctttgccgt tgtccgtgaa
     4921 actggcgccg aaaaatcgaa cgccccgtcg attactcgcg cggggcgccg actgaccagt
     4981 gatcccgatg cttgatggca acggatgact tacgagaatg ctaaggctga caatactgat
     5041 cgtcaacgag caattgtgat ggcgcggaag gtgggcaacg cggcggtagc ggagcgtgag
     5101 ggattcgaac ccccgggatc actgatcaag tcatcctagg gcttatcagg cccctgccat
     5161 cgtccactcg gccaacgctc cggcaagagc cgccgcgttg cccataactg gagccgaaaa
     5221 atcgaacgcc ccgacgattg ctcgccgagg cgccgactga ccagctgtcc cgatgccgtg
     5281 atggcagtcg gatggcttaa gcaaagacta aggacgctta gttgtcatcg tcaacgagcg
     5341 atcttccggt ggcacgagtc gaggcccggc ctcgctgcac gcagggagcg aacgtgcagc
     5401 gcacatgaag gagccagtac ccaaaccaga cgagaagcgg agacgataga cgcttctcga
     5461 cggagccggg ccccggctcg tgccgccgga aggatgcgta tcgatccgct cgccgccggc
     5521 gtgatgcggt gagagaggag cgacgacgag cggatcgaca cgcggatggt gtgacgtgac
     5581 gagctacttg agcgtggtgt cccccttgat gtcgaggaac ggctcgagtc gcttgaacgt
     5641 cttgtagccg aaacctttta cgcgccgcag atctgccgtg cgcttgaagc cgccgttctt
     5701 cttgcgccag gtgacgatgc ggtcggcctt tgcagggccg acggtcggca acatcatcag
     5761 ctgctcttcg gtcgcggtgt tgaggttgag cttgccggtg aggtccttcg cggacttctt
     5821 gatcggccgt tcgccgtcgt cgtcgccggc gctgctcgcg ctcgcgggct tcgcggcgat
     5881 cgcggccggt ggcgcagcgc tcgcgagatg ccacgtgccg cccatcgaga ggaacagcaa
     5941 gaacaacgcg agcatcgcga tgagcgggcg agtgagtgtg cgagtcgaga tggtcatggc
     6001 cggcctcaga acggaacggc atcgacggcg gcggggccgg tctgcgcggg tggcgtgaat
     6061 tgattcgaga gcgtgccgcg cgcgttgtac gtggccttct tttcgtcgcg ctcgcgcaat
     6121 tcctcgtcgg tgaactcgcg caactgcagc gtcacgcctt ggccgtcctt gctcggcacg
     6181 agcggaatcg cgtcgatgta ggcattgatc gagttgtcct tgttcacgta tgcggtgccg
     6241 agacgcatcc agaacttgcc gccgtcgcga cggtcgatgg gggtgatgac tttgaacatt
     6301 cgcttctgct gaaacacgac agtctcctta ctgacgccga tgcgtcgact gcgcgtaaga
     6361 gcaacggcaa tgccagcgct aagcctgcgg agttgctcgc gcgactgtcc ggggtccgac
     6421 ggaggccgga cggcgcgtcc ggatcgcgga cgaattgccg tcaagcggag tcgagcggag
     6481 tcgagcggag tcgagcggag cgctcagtgc ccgggcagcg agaccgagat gtgcgtcgtc
     6541 ttcgcgtcga ctttcaccga tcgtttgacg acgatcggcg cgccggacgt ccacgtgccg
     6601 tcgcgattcg cggtcgcgat cggcggctgc cagaacgtga tctcgtacgt gccgggtgac
     6661 agctcgtcga ggcggaaacg accttcgtcg tcggtgacgg cgtagtacgg cgtctcgagg
     6721 ccaagcaccc atgcgggcgc gaccttgcca tcctcgctct cgatcttggt gacacccgcc
     6781 ttgacgtcga cctgaacgag gccaccttct tggagctcct gggtcacctt gccgatgcgc
     6841 agcttcgtac gttgcgtgtc gccgttgatc gcgaccgagc cgggcagcgg cgcgacgatc
     6901 tgcgcggccg gtgcgagcgt gcagttgtgt ttcgccagca tgccgccgac ggatgcgggc
     6961 cgcgagtagt acggcgtgct gcgcccgatc gtgaccttct cgatgtagac gatcacgccg
     7021 cgcacggcct tgtccttgga cacgcgcagc gtcgggttgt cgatcagccc gcacgcggac
     7081 gcgaccttgg ccggcaactg gccattccac gtgaccgtgc cctcgatcgc accggtcagg
     7141 ccgatgctca cgctgtagcg cgggactcgg ttcggcacgt tgtacgacca ctgcggcatg
     7201 cgccagttcg cgtagcccga tccgccgtat ggatcgccgc cgaatccgcc gtacggatcg
     7261 ccgccgtaca tgtcaccgcc gtacgcgttg tcgatgtcgt accgcggtgg tccgcgctcg
     7321 tcgttgaacc cggcgacgct gtcgtcctgc gacgtttcga acttgcgcgc tgcgcgtgcg
     7381 agctcgccgg caccttcgtc cttgtcatcg cccggcggcg cgagcggaac acgttgcgag
     7441 ccgccgcacg cgatc
//
LOCUS       DASUFX010000614         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_516590,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000614 DASUFX010000000
VERSION     DASUFX010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516590"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFV99_24615"
     CDS             <1..511
                     /locus_tag="VFV99_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062587.1"
                     /translation="PAADVLEQRRDKVKLSVHDGHCTVQSREMEPTVLARAEAVDAIE
                     HDAPVGIPEPIKKPSKKKTAPVQQNVPPPAKTVDPKAAKLEKQRLLEEQKKLTSQQKV
                     APSKNGKPVQQPYDVNQANVGSGSRVTKGTQQAPQQAAQQAPQPQIVPEPKASVPQVN
                     EDDKARQKK"
     gene            555..1586
                     /locus_tag="VFV99_24620"
     CDS             555..1586
                     /locus_tag="VFV99_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062588.1"
                     /translation="MPGDSELRARAVLAEMLMRISQGPFAALSVHETATAEEVRAAFL
                     DLTKQFHPARYGRMSPDLQKMATEVFLGIKAAHDQMTKALGSTGRRRQVSAVPANATP
                     TGTERGVAAPRAGTPPIARTPPTRPSTPLITPSTGTPRPSTPVQPASRAPTPPGAPRA
                     PTPVGTSPRASTPVGLPRGQTPAVGVPVQRPSQPLITPHEPRHTPSQGMPASRPGSPP
                     ATGRPSTPAQRTPAPEIINPPTIRYSGVQPAQKGASDEDGMLEQAMNLLSSKDYAAAR
                     LAFHALAAKVPQSRHYRALLCYARGRETQTTGRADDAAMEFQRALQLDPDLEIAKEAL
                     RELGRKSRW"
     gene            1607..3166
                     /gene="dnaK"
                     /locus_tag="VFV99_24625"
     CDS             1607..3166
                     /gene="dnaK"
                     /locus_tag="VFV99_24625"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5062589.1"
                     /translation="MGRVIGIDLGTTNSCVAVLEAGEPIVIHNQEGGRTTPSMVSWNA
                     DGEVVVGAASKRQMVTNPQRTVFGVKRLIGRKAQDADVVALARRLPYKLVAARNGDAW
                     IDIAGTPRSPQEISAHVLAKMKRVAEDYLGQPVTEAVVTVPAYFDDAQRQATKDAGKI
                     AGLTVRQILNEPTAAALAYGLQHQNNQRLAVFDLGGGTFDISILAIENGVFEVLSTYG
                     DTTLGGDDFDRVIIDLLADEFQGQYGLDLRTDSVALQRLKEAAERAKIELSSAMTTDV
                     NLPFIAAGPTGPLHLMRELERGQLERACKTLFERLEAPCRRALAEAHCTPADIDQVIL
                     VGGMTRMPEVQERAERIFGKPGSKGVNPDEIVAMGAAVHSGIMGGELQEVVLLDVTPH
                     NMGVKVAGDRMSVVIQANTSIPTRAKKVFATTEDNQDFVAIEIYQGEHDLASKNRRLG
                     RFVLSDLTRASRGRTKVEVSFTMDADGILEVAAVEVGTGRASTVTIEASSGLTADEIR
                     QLASRQSRPGF"
     gene            3387..3791
                     /locus_tag="VFV99_24630"
     CDS             3387..3791
                     /locus_tag="VFV99_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062590.1"
                     /translation="MANIASEPRPAIVARPAFVAAVMLAAFALVPWQHHGGTRRVIVP
                     LDEIKRPPVIVPMELYAGPGVLADLVDLFAPAFDVAFDSVIEPAEHPDAQPWPRGMVI
                     HPPPFPDQMNVMPSGLDRLLSGLLAPWHSIAS"
     gene            3819..>6037
                     /locus_tag="VFV99_24635"
     CDS             3819..>6037
                     /locus_tag="VFV99_24635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5062591.1"
                     /translation="MSAESLAAFGEATRTWFSQSFAAPTPVQERGWASIASGKHTLML
                     APTGSGKTLAAFLYCLDRLARTREPGAGTRVVYVSPIKALAYDVERNLRAPLAGLQRL
                     GAGAQITVDVRTGDTSAKERERQRKDPGQILITTPESLYLLLAGRSRERLRSVDTIIV
                     DEIHALAPTKRGIHLGLTLERLSHLVTSSGHPEPQRVGLSATQRPLTEVARYLGGDRP
                     VEIIDASAKPALDLEIVVPAADMEQPGTLPTAEGKNDGGGMWPLIYPRLLDEILAHRS
                     TIVFVNSRRLSERVAQQLTELAVARGVAEPGVELVRAHHGSVARHQRLEIEEALKAGR
                     LRAITATSSLELGIDMGAVDLVLQVESPGAVSRGLQRIGRASHHVGGTSRGRILPKFR
                     GDLLEATVVAHEMLDGKVEAIRVPDSALDVLAQQIVAMVAADPWQVADLERVVRRAAS
                     YRELSHAAFLGVLDMLAGRYPSDEFAELRPRLTWDRQADILVGRKGARLLSVVSGGTI
                     PDRGLYGVHVGERGPKVGELDEEMVNESRKGETFILGATTWRIEDITRDRVIVSPAPG
                     EPGKMPFWRADGPGRPIELGKTLGAFCRELDAKLVNDPAAAQRWLEREYNLDAFAAGN
                     LIRYLADQRGQAGSVPSDRAITIERFRDELGDVRVCLLSPFGSRVHAPWALVLARQLE
                     DQLGYPIHPLWTDDGIALRFADGDLLPDDDQLLPDPDDVERVLVDELARSSVFASHFR
                     "
BASE COUNT         1054 a         2031 c         2040 g          912 t
ORIGIN      
        1 ccccgcggcc gacgtactcg agcagcggcg cgacaaggtg aagctcagcg ttcacgacgg
       61 gcactgcacc gtgcagtcgc gtgagatgga gccaaccgtg ctcgcgcgtg ccgaggcggt
      121 cgatgcgatc gagcacgacg cgccggtcgg gattccggag ccgatcaaga agccaagcaa
      181 gaagaagacg gcgccggtac aacagaatgt tccaccaccc gcgaagaccg tcgatccgaa
      241 ggccgcgaag ctcgaaaagc agcggctgct cgaagagcag aagaagctca cctcgcagca
      301 aaaggttgca ccctcgaaga atggcaagcc ggtgcagcag ccgtacgacg tcaaccaggc
      361 gaatgtaggt tctggttcga gggtgacgaa gggcacgcag caggcgccgc agcaagcggc
      421 acaacaagcg ccgcaaccgc agatcgtgcc ggagccaaag gcgtctgttc cgcaagtcaa
      481 cgaagacgac aaagcgcgcc aaaagaagtg accggctttc ctcgccgtac ccttcgggct
      541 acgatcgggg agcagtgcca ggtgacagcg aactgcgagc gcgagccgtg ctcgccgaga
      601 tgctgatgcg catctcgcag gggccgttcg cagcgctgag cgtgcacgag acggccaccg
      661 cggaagaggt gcgcgcggcg ttcctcgacc tgaccaaaca gtttcatccc gcgcggtacg
      721 gtcggatgtc gcctgatcta cagaagatgg cgacggaggt cttcctaggc atcaaagccg
      781 cgcacgacca gatgacgaag gcgctgggct cgaccgggcg gcgccgacag gtgagcgctg
      841 tcccggccaa cgcgacaccg accggcaccg agcgaggggt tgcggcgccg cgcgccggaa
      901 cgccaccgat cgcacgcacg ccaccgacgc ggccgtcgac gccgctgatc acgccatcga
      961 ccggcactcc gcggccgtcg acacctgttc agcccgcgtc ccgtgcaccg acgccgccgg
     1021 gtgcaccgcg cgcaccgacg ccggttggaa cgtcgcctcg cgcgtccact ccggtcggcc
     1081 tgccgcgcgg gcaaacaccg gccgtcggtg tcccggttca acgcccgagc cagccgctca
     1141 tcacgccgca cgagccacgt cacacaccgt cgcaaggcat gcccgcatcg cgaccagggt
     1201 cgccgccggc gaccgggcgg ccgagcacac cggcgcaacg aacgccggcg cccgagatca
     1261 tcaatccacc gacgatccgg tacagcggcg tgcaaccggc acaaaagggt gcctccgacg
     1321 aagacggcat gctcgagcag gcgatgaatc ttttgagctc caaggattat gctgccgcgc
     1381 gactcgcatt tcatgcgctc gccgcgaagg ttccgcagtc gcgtcattac cgagcgctcc
     1441 tgtgttacgc ccgcggccgt gagacgcaga caacgggcag ggcggacgac gccgcgatgg
     1501 agtttcagcg cgcgttgcaa ctcgatccgg atctcgagat cgcgaaagaa gctctccggg
     1561 agctaggccg caagagtcgg tggtagcagt gttatagtgg atgcgagtgg gacgcgtcat
     1621 cggtatcgac ctcggcacga cgaactcgtg cgtcgccgtg ctcgaagcag gcgagcccat
     1681 cgtcattcac aaccaggaag gcggccgtac gacgccgtcg atggtgtcgt ggaacgcgga
     1741 cggtgaggtc gtcgtgggcg cggcgagcaa gcgccagatg gtcacgaacc cgcagcgcac
     1801 cgtgttcggc gtcaagcgcc tgatcggccg caaggcccag gacgcggacg tcgtcgcgct
     1861 cgcgcggcgg ctgccgtaca agctcgtggc cgcgcgcaat ggcgatgcgt ggatcgacat
     1921 cgcgggcacg ccgcggtcgc cgcaagaaat ctccgcacac gtgctcgcga agatgaagcg
     1981 cgtcgccgag gactacctgg gccagcccgt cacggaggcc gtcgtgacgg tgccggcgta
     2041 cttcgatgac gcccagcggc aggccaccaa ggatgccggc aagatcgctg ggctcacggt
     2101 ccgccagatc ctcaacgagc cgaccgcggc cgcgctcgca tacggtctgc agcatcagaa
     2161 caatcagcgg ctcgcggtgt tcgacctcgg cggcggcacg ttcgatatct cgatcctcgc
     2221 gatcgagaac ggcgtgttcg aggtgctatc gacgtacggc gacaccacgc tcggcggtga
     2281 cgacttcgat cgcgtgatca tcgatttgct cgctgacgag ttccagggcc agtacggcct
     2341 cgatctgcga accgactcgg tggcattgca gcgcctgaaa gaagcggcgg agcgcgcgaa
     2401 gatcgagctg tcatcggcga tgacgaccga cgtcaacttg ccgttcatcg cggccgggcc
     2461 gaccgggccg ctgcacctga tgcgcgagct cgagcgtggt cagctcgaac gcgcgtgcaa
     2521 gactctattc gagagactcg aagcgccatg ccggcgcgcg ctcgcggagg cgcattgcac
     2581 gccggcggac atcgatcagg tgatcctggt cggtggcatg acgcgcatgc ccgaggtgca
     2641 ggagcgggcg gagcgcatct tcggcaagcc gggcagcaaa ggtgtcaatc ccgacgaaat
     2701 cgtcgcgatg ggcgctgccg tgcactcggg catcatgggc ggcgagctcc aggaggtcgt
     2761 gctcctggac gtcacgccgc acaacatggg cgtgaaagtc gccggcgacc gcatgtcggt
     2821 cgtgatccag gccaacacca gcattccgac gcgcgccaag aaggtcttcg cgaccacgga
     2881 agacaaccag gacttcgtgg cgatcgagat ctatcagggc gagcacgacc tggcgtccaa
     2941 gaaccggcga ctcggccggt tcgtgctcag cgatttgacc cgcgcatcgc gtggccgcac
     3001 caaggtcgag gtcagcttca cgatggatgc agatggcatc ctggaggtcg ccgcggtcga
     3061 ggtcggaacg ggccgcgcat cgaccgtcac gatcgaagca tccagcgggc tcacggcgga
     3121 tgaaatccgg cagctcgcgt cgcgccaatc gcggcctggt ttctgactct ggcaatgcgc
     3181 gcgggtccag cgcgctggtt cggatgcaga ggttggtcgg ggcgtgcgcg gcgccggcta
     3241 gagcttccct cgatgtacag gtcgcgtcgc agaatcggtg ttatgtcaac tccggtatac
     3301 ggcaactggg cacaacctca gggtttggcc ccgccgccga tctgctgcgc tgggaatgaa
     3361 gctcgccgcg gtccggttcc gcggtcatgg cgaacatcgc gagcgaacca cggccggcga
     3421 tcgttgcgcg gcctgcgttc gttgccgcgg tcatgctcgc cgcgtttgcg ctcgtgccgt
     3481 ggcaacacca cggtggaacg cggcgcgtga tcgtgccgct tgacgagatc aaacggccgc
     3541 cggtgatcgt gccgatggag ctctatgccg ggcccggcgt gctcgcggac ctcgtggact
     3601 tgttcgcgcc cgcgttcgac gtggcgttcg actccgtgat cgagccggcg gagcatccgg
     3661 acgcgcagcc ctggccccgc ggaatggtca tccacccgcc cccgttcccc gaccagatga
     3721 atgtcatgcc cagcggattg gacaggctgt tgtcaggttt gctcgcgccc tggcattcca
     3781 tcgcttcgta gccctgccgt tgttgctata cgagcctggt gtcggcggag tcgctcgcag
     3841 cgtttggcga ggccacgcgt acgtggtttt cgcagagctt tgccgcgccc acaccggtcc
     3901 aagagcgcgg ctgggcaagc atcgcgagcg gcaagcacac gctcatgctg gcgccgaccg
     3961 gcagcggcaa gacgctcgcc gcgttcttgt actgcctcga tcggctcgcg cgcacgcgcg
     4021 aacccggagc cggcacgcgc gtcgtctacg tttcgccgat caaggcactc gcctacgacg
     4081 tcgagcgcaa cctgcgggca ccgctcgccg gcctacagcg actcggcgcc ggcgcgcaga
     4141 tcacggtcga cgttcgcacc ggcgatacat cggcgaagga acgcgagcgc cagcgcaaag
     4201 accccggcca gatcctgatc acgacgcccg agtcgctcta cctgctgctc gccggccgat
     4261 cgcgcgaacg gctgcgcagc gtcgacacga tcatcgtcga cgagatccac gcgctcgcgc
     4321 cgaccaagcg cggtattcat ctcggcctca cgctcgagcg gctctcgcac ctcgtcacgt
     4381 cgtccggaca ccccgaaccg caacgcgttg gtctgtcggc gactcaacga ccacttacgg
     4441 aagtggctcg atatctgggc ggggaccgac ccgtcgagat catcgacgcc agcgcgaagc
     4501 ccgcgctcga tctcgagatc gtcgtgccgg cggccgatat ggagcagccc ggcaccctgc
     4561 cgaccgccga gggcaagaac gatggcggcg ggatgtggcc gctgatctat ccgcgcctgc
     4621 tcgacgagat cctcgcccac cggtcgacga tcgtgttcgt caactcgcgc cggctctccg
     4681 agcgcgtcgc gcagcagctc accgagctcg cggtcgcgcg cggcgttgcc gagccaggcg
     4741 tcgagctcgt gcgtgcccac cacggctcgg ttgcgcgcca tcaacggctg gagatcgaag
     4801 aagcgctcaa ggccggccgg ctgcgcgcga tcaccgcgac gtcgtcgctc gagctcggca
     4861 tcgacatggg tgccgtcgat ctcgtgctgc aggtcgaatc gccgggtgcg gtgtcgcgtg
     4921 gtctgcagcg catcggtcgc gccagtcacc acgtcggcgg cacgagtcgc ggccgcatcc
     4981 tgccgaagtt ccgcggcgac ttgctcgaag cgaccgtggt cgcgcacgaa atgctcgacg
     5041 gcaaggtcga agcgattcgc gtcccggata gcgcgctcga cgtgctcgcg cagcagatcg
     5101 tcgcgatggt cgccgcggat ccgtggcagg tcgcggacct cgagcgcgtg gtgcggcgcg
     5161 cagcgagtta tcgcgagctg tcgcacgcgg catttctcgg cgtgctcgac atgctcgccg
     5221 gtcgctatcc gagcgacgag ttcgccgagc tgcggccacg cctcacgtgg gatcgccagg
     5281 ccgatattct cgtcggtcgc aagggcgcac ggctcctcag cgtggtgtcg ggcggcacga
     5341 ttcccgatcg cgggctctac ggcgtgcacg tcggcgagcg cggccccaag gtcggcgagc
     5401 tcgacgagga aatggtcaac gagagccgca aaggcgagac gttcatcctc ggcgcgacga
     5461 catggcggat cgaggacatc acgcgcgatc gcgtgatcgt gagcccggcg ccgggcgagc
     5521 ccggcaagat gccgttctgg cgtgccgacg gtccgggccg cccgatcgag ctcggcaaga
     5581 cgctcggtgc gttctgccgc gagctcgatg caaagctggt gaacgatccg gcggcggcgc
     5641 agcgctggct cgagcgcgag tacaacctcg acgcgtttgc ggccggcaat ctgattcgct
     5701 acctcgccga tcaacgcggc caggccggca gcgtgccgag cgatcgcgcg atcacgatcg
     5761 agcggttccg cgacgagctc ggcgacgtgc gcgtgtgcct gttgtcgccg ttcggcagcc
     5821 gcgtccacgc accatgggcg ctggtgctcg cgcgccagct cgaagaccag ctcggctatc
     5881 cgattcatcc gctgtggacc gacgatggca tcgcgctccg gttcgccgac ggtgatctgt
     5941 tgcccgacga cgatcagcta ctccccgatc ccgatgacgt cgagcgcgtg ctggtcgacg
     6001 agctcgcacg ctcgagcgtg ttcgcgagtc acttccg
//
LOCUS       DASUFX010000615        21887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1766397, whole genome shotgun sequence.
ACCESSION   DASUFX010000615 DASUFX010000000
VERSION     DASUFX010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766397"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFV99_24640"
     CDS             complement(<1..498)
                     /locus_tag="VFV99_24640"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5062592.1"
                     /translation="MSDAKGLATSASDPMQSLAAALRREQKKVALVQEVSRALSTVGD
                     IDTLLRLIMEKVTELMEADRSTLFLVTEDGRQLWSKVIQGSEVVEIRLDVGEGVAGWV
                     AQTREIVNIPDAYADQRFQPAVDLKSGFRTRSILSVPMLGALGGLVGVLQVLNKHDGP
                     FTTPAE"
     gene            complement(550..1794)
                     /locus_tag="VFV99_24645"
     CDS             complement(550..1794)
                     /locus_tag="VFV99_24645"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5062593.1"
                     /translation="MRWVLAAALLVGCKGTPNKPAEPAHSETVAAATTPVPTHAEATE
                     ATVRPHHEQPVVECAAKIPDNADDETRVQALLDQANKEIERGQYAAAWTCADRASDIV
                     PSSVEAHHLRGASLAALGRDGEAQMAFQLALALDPDDPETLRAVADFYINGKGDRTRD
                     ALRLGLELAQRGSRRALARRRRNAPLAADLAVLEAQALNDLGRSDEALERVDSALRIT
                     PGRGDALHERGVALFDLSRFSEAKVAFEKALGIQPQDAYTHQMLGLTLEQLGEGNTAD
                     KELAKAIELAPKELSAPVLITVTDFQKEIDAVVASLPADRRAHVKSIKLEIADLPDPV
                     DLKAVQPPFPPTILGLYRGPVGRAPAPDDEAPSIVLYRKNLARAVKTRTELSEQIRDT
                     LLHEIGHLEGLDEDDLRRRGME"
     gene            1893..2549
                     /locus_tag="VFV99_24650"
     CDS             1893..2549
                     /locus_tag="VFV99_24650"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HEX5062594.1"
                     /translation="MSIEGELWHEVEAKRDDGTPTMFRIRELDRKPTLTRIFVVELPY
                     PKTELSRLPNASDYRRLAEFDEQWLRPACTSLGWEYVGHKIEDGSFFFYTYGCADPNA
                     MIEKLSPYDAGLGFYDDEDPDWQEYGTLKNLLEQAKALPAAAPKPNARTRTTTTAATP
                     MAKPNAKAKAKRNAKATPKAAAKTKTKLKANAKAKAKVKVKVKVKATAKAKPAKRRRR
                     "
     gene            2649..5120
                     /locus_tag="VFV99_24655"
     CDS             2649..5120
                     /locus_tag="VFV99_24655"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5062595.1"
                     /translation="MADRPDGSWDAPTEHDDQHEIDSGEFGAEDPPTVKATESQRARA
                     RGDAIANQLASVNRNVVAKPAAPLPAPPMPLPSTTLPPPVVQRAPAKPSTPSDSIVML
                     DKVSSKAAAEPAPRRGESAAGGIVVVATGGDAERIRKLCHKHGLLVPVIGSLAAVHVA
                     VSIVVVGEPSPPAPERVVHVVRPTVPDDKLVDLLRALVSGRVIVEPPEAVVPINSRVG
                     EAVRRLATLSDRGAIEMTTIEAITAITACDRAHCLFYDPSTSALWSEAKRREGSDDRQ
                     AMGGIVGWAALTGQTIYASPAGDDPRWLQEVDDPDGKPQSRVLVQPIIGADRRVHAVL
                     IAIRRWRHADFSDVERNALASFAALAGPALDLAVAASPPPTPRKARSTLPGAAASKSA
                     IVSTPRAASESRPPPVTARSGPEAGPPGSMTRGRDNARSHKPSTQPPPTVELPPPSDE
                     FTSVDDNDRYSSDSILSLPSPPHLPAPIDQPPVPYKTVKPAARKRPRTHSDSQAPKPR
                     GDGDLVDLAVVAIDDDARRVQRLVKKTRFEISTFARLADVPDFYRVLTLGDAWQGDDA
                     RIIYAARSSIPDDQLNDLFDALVHDRVVAPAGPLTAPQSIAEARRSQIALAGARKLAV
                     AVDLAAAETITVETMRELLDTDRAYCWIFEPDAGLMWSEARRRVGIEPFRAIAGITGY
                     CARTGKAANVPHASADPRWLAPLDDPDGDPHSQLLVQPVVRNDGRVHGLLVAVRRPRR
                     PGFTDLDVTLAARFAGLVAPLFEQIAVTQTSQELVGEDTSARPPIEPRAQSSVSMMTR
                     VENPIIRWVFLAIGLAVGVIIGVML"
     gene            complement(5117..7864)
                     /gene="polA"
                     /locus_tag="VFV99_24660"
     CDS             complement(5117..7864)
                     /gene="polA"
                     /locus_tag="VFV99_24660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5062596.1"
                     /translation="MAERLYIVDGHGYIYRAHYGLMNVSRGERREVRLSTSEGMPTGA
                     LYVFSRMLMRLEEDFKPERMAVVFDDKSGKKTFRADMYADYKAHRKETPEELQVQMEY
                     FPKVVGGLGWPCLAVPGVEADDVIATLVVEAKKKGWEVVIFSADKDIMQLIGDGVTMI
                     DALHQKTYTREEVIKKMGVPPEKIPDFLALVGDTSDNIPGLRGVGDKTAANLLEQYGT
                     LDNLIKTNPVVPRLSVKQPFGDPEMLERVKISRQLVELKRDVPLQPLEELVVGQWDLP
                     VLLQLFTELEFQVLVEKVKMKMPPGEDMIVVPAPEATPLATVEARQDAKVIVRGDAIA
                     ELAKHAEAAGKLAITVELDPERIERARMVSVIVAVPGQAPAYIPLAHRYIGAPSPPVA
                     GDLVPLHRVLEDPTVAKICHNAKQVVRAFLDIGVTVAGIVEDTMLAAFLLDPTADAEP
                     GEAVAQRLGGVLLPGRDKIVGRAKSLEAVEVERAAPWTGAITYSLLPIADELPARLDA
                     SGLAALYREIELPVANLLARIERVGIHIDTNHFKKLGAEVSARLEGLEKQIYEIAGEE
                     LNVSSPKQLGELLFEKLGLATKGVRRTKTGWSTEADTLEQLEHPIIKPILEHREISKL
                     KGTYLDALPPLVSSKTGRVHTTFNQVVAETGRISSQDPNLQNIPIRSDLGMQIRRGFI
                     AAPGHVLIAADYSQIELRILAHLSGDPLLSSAFRDHVDVHTQTAAEVFGVPREQVTAE
                     QRRVAKAVNYGLSYGQSDFGLARALDVTRTQAAEYSRRYFQRFPTIHKFMDEIVAVAR
                     AQGGARTVLGRWRPIPNLMSKSPAARHAAERVAQNTPMQGTGADIIKLAMLRTTERIA
                     REGWPVQMLLTVHDELVFESPPDIADTVGAALKDEMENVYKLSVPLEVDIGIGTNWAD
                     A"
     gene            7953..9092
                     /locus_tag="VFV99_24665"
     CDS             7953..9092
                     /locus_tag="VFV99_24665"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5062597.1"
                     /translation="MRAAAVALLVSACSFNHGLGAFDASGGGGDDTGSGSGSGSDAMM
                     GSDGGGSGSDGPMSTLRQKTITIGTIAGGPHADFPVWITLSDADLMARARTDGTDIHF
                     LIGGQPADYQIQSWVKNTGRLDAWVRVPSLDTGTQIVMRYGDVSVAHTPDVPGTFGGY
                     QAVWHLDDALNNTTVADARNLHNGTAVMLQANDSTAAQLGNGINFSDGNDQITFTNPL
                     AGNSAHTISFWINQRATASNDCVIAMGNGQLNEARWFHSRYNASTVAVGFYTNDWDNP
                     AENIIGGNWTLMHWVYEGNRMSRIYRNGVLVAGPFMHQNNIDTMGTAGSIGNAPVAYG
                     ANMGLNAALDEMRIINVVRNANWIAMEYKNQKTPSMFYTVGPEMQ"
     gene            9137..10207
                     /gene="plsX"
                     /locus_tag="VFV99_24670"
     CDS             9137..10207
                     /gene="plsX"
                     /locus_tag="VFV99_24670"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5062598.1"
                     /translation="MHRIVVDAMGGDHAPDEVVRGAAEASLALTNAEIILVGDAAVLG
                     RLLPRMRHDGARVRVHHAPAFVEMDEKPAEALAAKPEASIAVAADLVARGEGDALVSA
                     GNTGASVLACARRWTLLTGVRRAALAAVYPTELRRGEKDDPFSLILDVGATIDATAED
                     LVGFAVMGSAYAKLISQNRRPRVALLSNGTEAGKGPPEVVRAHEQLVETTELNFIGNI
                     EGLDIPRGVADVVVTSGFVGNVVLKMLEGVSETVVRLARYAHKERLAWRLGLVALSSA
                     IDQLKEVTDWQQYGGAPLLGFTHPFIKAHGRSNARAVQNAVKVAHKALAGNLCGNIAR
                     TMAELDERAQRRTTSQGDVVGA"
     gene            10207..11328
                     /locus_tag="VFV99_24675"
     CDS             10207..11328
                     /locus_tag="VFV99_24675"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX5062599.1"
                     /translation="MFRRISKLPVRIGERIGLFEPATPQLAPGERLPKRTFRWDLDKT
                     YLRTEFDSLKDLAKSAIETAADKQAYPGATALLRALRQDGNRICIVSGSPTQMRQVLA
                     AKLALDGVEYDEFVLKNNLKNILRGRFRALRAQIPYKLPAMLSSRINVPAPSEMLFGD
                     DAEADAIIYCLYADLIAGEVSLTDLERVLIASRAYDDDAERCLDLARRVPQAKAVTRM
                     FIHLDRRSPPMGFRRFGPRLVPVFNYFQAALVLYEDQVLTARQVIFVALEMLDSGQFA
                     LGHLATSVQDVIRRGRVTRESALRLTEEAAEAARSGALSGRSDLPPFEKIAESFRERV
                     RELGGTVAPEPAVAEPLDYVRLVDEEHEHRHRRRRQGMG"
     gene            11440..12897
                     /locus_tag="VFV99_24680"
     CDS             11440..12897
                     /locus_tag="VFV99_24680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062600.1"
                     /translation="MRLCIAAVVLAACGGGGGTQAPDASPPDAPIDMPPMFAACREFG
                     GSTATVPAHVTGSLAGPDVQSPSQCTAVDAPYGIESAGPDAVVRIDGLVPGTAYIVQL
                     DSPEDLAFYVVSGCSTPSGPAANECSLFVDASAGSEEVGRFVAASTTTYIVVDYYASH
                     TPANQTFALDVYAEACTTNSQCSAGAPVCSNGKCVQCASSFDCVDSQAPVCDRTAQTC
                     GPGSDMCLSDDSVEPADDGPAGARALVPNGGGFASATGEICSTPRSEADFYSFQVTSV
                     GEVWDFSLLWAGTDDLDLEIFDANGSAYGLSYWEQPEAARLTYLPTGTYFVKISDFTS
                     QTTTPVAYTLSARRTAGPGCTTRADCASEYRNQIFRGDCVAGACVKIEGAGLVEEGGA
                     CDSVSDCGSMLSCPSFFFVANADTRDVCARTCSGDADCAALGESYVCTTYLIRNFCVQ
                     KCTTNEQCATSLDSTPMSGPWYRLTCTPATGRCAP"
     gene            12916..14346
                     /locus_tag="VFV99_24685"
     CDS             12916..14346
                     /locus_tag="VFV99_24685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5062601.1"
                     /translation="MDRSGSILVVEDDAAMRELLTEELADAGFTVQSAGSAAGGLEIA
                     RSGRFELVVTDLRMPEMDGFDLIRGVMALPEPPHIIMITAFGSIETAIRAVKLGAYDY
                     ITKPFEIEELLLVADKALGERALRSKVARLQREVEKEYGLGGIIAKSQSMRDVISDVR
                     RIAGSTASVLITGESGTGKELIARAIHYNSSRAAGPFVGVNLAAVPEGLIESELVGHK
                     TGAFTDARNDKPGLFVEADAGTIFLDEIGELALPLQAKLLRVLQEHEVRPLGATKNTR
                     IDVRVVAATNKNLGAMLADGSFREDLYYRINVINLDLPPLRSRPEDIIPLAEHVLAQL
                     GAKQQPQRRMRLSPEAQHLLLAYHWPGNVRELMNVLERGVALCQGELIADDDLPQHVR
                     ERRPADFLGAAVARRMTLSELEREFIERVLEDEAGNKTRAAQRLGLDRKTLYRKLDEY
                     AKQDGKAEAPTRPSHPDKPDKDDDES"
     gene            14442..14951
                     /locus_tag="VFV99_24690"
     CDS             14442..14951
                     /locus_tag="VFV99_24690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062602.1"
                     /translation="MQKLQLCAVALALAACASDTDDPSNEPRRGKGPHTPEPTVDAAL
                     TPPPPSDPVGTVSCYTEGAPQAACTLPTHCCFGNYSAQHNGYCTTEACAESTIACDGP
                     EDCANGQHCCATLLDIGRWNLACQSSACSPQPLGDELCHDSSTCGGGTCVSALDANYD
                     LPRTLSVCR"
     gene            complement(14948..15616)
                     /locus_tag="VFV99_24695"
     CDS             complement(14948..15616)
                     /locus_tag="VFV99_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062603.1"
                     /translation="MSEVRDLIVDSDERVGDGGFLAIRRLHLRNVHGDGSTSAQYICD
                     AVVRPFGQDAVVVVVYARTERGTEVLVRHGLRPALVLGRDPARAPLPEQAPGLFLTEL
                     VAGIVEEGDQGELGLKARAAHEVLEEAGFLVEQDKVVLLGAGMFPSPGCLVEKFYFTA
                     VEVDPATQQPLTGDGSPMEEGARTTWLILDDAIAACRAGALTDLKTEVGLRRFREYLR
                     ETAR"
     gene            complement(15613..16251)
                     /locus_tag="VFV99_24700"
     CDS             complement(15613..16251)
                     /locus_tag="VFV99_24700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062604.1"
                     /translation="MQYLVALLTVAAVACGPSSKEVGTAKAARYQGDKLTLFAATKAA
                     VESKYKLQKSDETTLGMQTEGRWFTPEGMAVTREGGNGTSTRDMGGGMTRTDADSQFP
                     DGSVNITLVVTLLPDGSNWIVKVTPVMLRFHAGMPKPDPVPENDPSVPGWATSKVDQL
                     AVDIHSALAKYEVKQLGGLPPPPAPGPSPDEPSAGSAAAPPAAGSAAGSAAP"
     gene            complement(16265..16753)
                     /locus_tag="VFV99_24705"
     CDS             complement(16265..16753)
                     /locus_tag="VFV99_24705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062605.1"
                     /translation="MRVLLLLQIVALCMSCGPSAAEIKTAKTAHYRGNASEMFNEVIA
                     VTQETYKIANANGGDEDSYVLATVPQWYTAEGGRESAGDQGYTQIRDHSVRLELIVEL
                     VSVAGGFMVTVTPKTFQALSGSPQPRELKPDDPNLPGWVPGRVDSLQLEIHKRLQNYA
                     VQ"
     gene            16775..17599
                     /locus_tag="VFV99_24710"
     CDS             16775..17599
                     /locus_tag="VFV99_24710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5062606.1"
                     /translation="MSELKGKRALVTGASSGIGAEIAHELARRGVHLVLAARRRQHLD
                     DVASACAEHGVATEVITADLGTAGGARALWSTATRTPVDILINNAGFGYFRPFAEIDW
                     ARDAELVQLNITSLVELSKLFREHRQGRDGRAYLVNIASIAAYQSVPNMALYTSSKAF
                     VRNFTEALHDEWKGTQLSATCICPGGTKTDFHAMAGAGDYGWLANVSMMTAERVARIS
                     VDAMARGARNVIPGFMNKLSCWGVRLVPRRFASWMSRRVLGKPRPGELPARTTEAA"
     gene            17596..18606
                     /locus_tag="VFV99_24715"
     CDS             17596..18606
                     /locus_tag="VFV99_24715"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HEX5062607.1"
                     /translation="MTERHNVRHWTNDTRGVYEVWYLTWNHPATDQGFWLRYITEAPV
                     DGPARGELWFARFDPKNPARTFGIHKRFPIDQLQSAADPFSLSIAGSRLGHDHAIGQL
                     AGNGHDVRWDLRWDAAAQVLRLLPDVMYARGGLGETTVQSPNPRVAMTGTLVVDGEKL
                     TFDRATLGQTHLWGKKHAYSWTWGRCAEFAGAPDALLEILAVRLQRRGTTLPPMTLVT
                     LNLDGEQYRLNQFRHVVRNRGTWANGHVTFSGWSPTVRIEGELSCRPDQMVSAPYLDP
                     DGTEVFCANTEIGDARVTVFRRSGLRWREHRTIESKGHAHFELGGRTRDPAVEREHML
                     VP"
     gene            18623..19738
                     /gene="gcvT"
                     /locus_tag="VFV99_24720"
     CDS             18623..19738
                     /gene="gcvT"
                     /locus_tag="VFV99_24720"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5062608.1"
                     /translation="MEPRKTPLWETHKKLGAKLVDFAGWHMPVSYPAGTIKEHKAVRE
                     ALGLFDVSHMGEAYLRGPRAREVVAKLATNACESAPPGKAVYTLLCRPNGGIVDDCIF
                     YKKSDDDFMVIFNAGNVAKDLDWFREHASTTCDVIDVSDETALIAVQGPKAVGVVDKL
                     GDGTLSSMPSFTFRDTTIAGVKCMAARTGYTGEDGFELACANADATKLWNALLDAATP
                     AGGLPIGLGARDSLRLESKLPLYGNDLDDDHTPLEAGLGWAVKLDGRNFLGSQALRDQ
                     KAKGLTRQLVGFKIDESVRAIARHGYPIVDRSLAAADQVIGTVTSGGPGIYVGGAIGL
                     GYVPTALAAAGTKLTIDCRGKDATATVVSGKFYKRPQ"
     gene            19746..20132
                     /gene="gcvH"
                     /locus_tag="VFV99_24725"
     CDS             19746..20132
                     /gene="gcvH"
                     /locus_tag="VFV99_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5062609.1"
                     /translation="MSTFPTELRYTNDHEWIRVQGTSWRVGITQFAVDALGDITLVDL
                     PKEGDQVTKGQRFGTVESVKSVSDLYAPVSGRVVAVNSALKDAPEDVNAEPYGKGWMI
                     EVEPNDKGELDELMTADAYTQHVAAQ"
     gene            complement(20158..21186)
                     /locus_tag="VFV99_24730"
     CDS             complement(20158..21186)
                     /locus_tag="VFV99_24730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5062610.1"
                     /translation="MLFEQIRSGGCLSYLIGCDDSCAAMVVDPHLDQLDRYLALIAER
                     GMRVHYIYETHTHADHFSAARELGKRLGVHVVMHRNAAAPYADMRVDDGETLIVGKLR
                     VKVLHTPGHTNDSTCLVFEDRVLTGDTLLIGGTGRTDLPTGDASALYDSLFGKLLKLP
                     DSMKVYPAHDYKDRSSSTIGAEKASNPRLQKKERADFVALMHSLDLKMPDHLSEALRT
                     NRSGGKTVSQLLAEASRDIAFMSIDDVRDRIASGGDGLVLLDVREKEAFNNGHMPGAQ
                     HLPRGQLELRVDKELPDPSAHILTYCQFGRISTLAAATLRAMGYPRAVALDGGFDEWV
                     KRGFPIEK"
     gene            complement(21195..>21887)
                     /locus_tag="VFV99_24735"
     CDS             complement(21195..>21887)
                     /locus_tag="VFV99_24735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEX5062611.1"
                     /translation="KQNARDGFAAFYTALFDATLAKHPGAVITEYAWDATTCEPCPGP
                     TLDYAAFQALGGDVLDGTAASPTAYGSSDFVLTRMHVRYDKSVKNDLTFKEVPPIAGG
                     REQRGPDGAVEQSAQASSYSAFQARYAIRNRWLGPLTCSNPVRGRWARKGAIQVAQDL
                     AYAPRGTLQLADQVAQDVPELDLTIGKEPPPTTTLARSPEKESGCNCSAGGASGGLAI
                     GVLVLLVRRRKR"
BASE COUNT         3665 a         7376 c         7263 g         3583 t
ORIGIN      
        1 ttcggcgggg gtcgtgaacg gaccgtcgtg cttgttgagg acctgcagga caccgacgag
       61 accacccagc gcgccgagca tcggcacgct gaggatcgag cgcgtgcgaa agccgctctt
      121 gagatcgacc gcgggttgga agcgctggtc ggcgtacgcg tccgggatgt tgacgatctc
      181 gcgcgtctgc gcgacccagc ccgcgacgcc ctcgccgaca tcgagccgga tctcgacaac
      241 ctcactgccc tggatgacct tcgaccagag ctggcggccg tcttcggtga ctaaaaagag
      301 cgtggagcga tcggcctcca tcagctcggt caccttctcc atgatcaggc ggagcagcgt
      361 gtcgatgtcg ccgaccgtgg acagcgcgcg gctgacctcc tggaccagcg cgactttctt
      421 ctgttcgcgg cgcagggcgg ccgcgagcga ctgcatcggg tccgatgcgc tcgtagccag
      481 tcccttggcg tccgacatcc cacccagcat ggtaccagag cggccgatcc gaccgctcgg
      541 cgggtggctc tattccatgc cgcggcggcg caggtcatcc tcgtcgaggc cttcgaggtg
      601 accgatttcg tggagcagcg tgtcgcggat ttgctcggat agctcggtac gtgtcttcac
      661 ggcacgtgca aggttcttgc gatagagcac gatcgagggc gcctcgtcat ccggcgccgg
      721 cgcacgcccg accgggccgc gatacaggcc caagatcgtc ggtgggaacg ggggctgaac
      781 cgccttgaga tcgaccggat ctggcagatc ggcgatttcg agcttgatcg atttgacgtg
      841 cgcgcgccgg tcggctggca gcgaagcaac gaccgcgtcg atctctttct ggaagtcggt
      901 gaccgtgatc aggacgggag cgctgagctc cttcggcgcg agctcgatcg ctttggcgag
      961 ctccttgtcg gccgtgttgc cctcgccgag ctgttcgagc gtcaggccga gcatctggtg
     1021 cgtgtaggca tcctgcggct ggataccgag cgccttttcg aacgcgacct tcgcctcgga
     1081 gaaccgcgac agatcgaaca gcgcgacgcc gcgctcgtga agtgcgtcgc cgcgacccgg
     1141 cgtgatgcgc agagcgctgt cgacgcgctc gagcgcctcg tcgctgcggc cgagatcgtt
     1201 gagcgcctgg gcttcgagca ccgcgaggtc cgcggcgagt ggcgcgttgc gacgacggcg
     1261 agcaagcgcg cgccggctgc cgcgctgggc gagctcgagg ccgagccgga gcgcgtcgcg
     1321 ggtgcggtca cccttgccgt tgatatagaa gtccgcgacc gcgcggagcg tctcggggtc
     1381 gtccgggtcg agcgcgaggg cgagctggaa cgccatctgt gcctcgccgt cgcggcccag
     1441 agccgcgagc gacgcaccgc ggaggtgatg cgcctcgacg gagctcggca cgatatcgga
     1501 ggcgcggtcg gcgcacgtcc acgcggcggc gtactggccg cgctcgattt ctttgttcgc
     1561 ctggtcgagc agcgcctgga cgcgggtctc gtcgtcggcg ttgtcgggaa tcttggcggc
     1621 gcactcgacg acgggctgct cgtggtgcgg gcgcacggtc gcctcggtcg cctcggcgtg
     1681 cgtcggtacc ggtgtcgtcg ccgcggcgac ggtttcacta tgcgcaggct ccgcgggctt
     1741 gttcggtgtc cccttgcagc caacgaggag agctgccgcg agcacccaac gcatggcgcc
     1801 cattttagcg tcttcgggtc tggaacgcga aacctgcaag tcgcggagat gactcgacct
     1861 accgcgagga tttcggagta ctttatgcgc tgatgtcgat tgagggcgag ctctggcacg
     1921 aagtcgaagc caaacgcgac gacggcacgc cgacgatgtt tcgcattcgc gagctcgatc
     1981 gcaagcccac tctcacgcgg atcttcgtcg tcgagctgcc ctatccgaag accgagctgt
     2041 cgcggctgcc gaacgcgtcg gactatcgcc gcctcgccga attcgacgag caatggctgc
     2101 gtccggcgtg tacctcgctc ggctgggagt acgtcggcca caagatcgag gacggatcgt
     2161 ttttcttcta cacctacggg tgcgccgatc cgaacgcgat gatcgagaag ctgtcgccgt
     2221 acgacgccgg cctcggcttc tacgacgacg aggatccaga ctggcaggag tacggcacgc
     2281 tcaagaatct gctcgagcaa gccaaggcgc ttccggccgc agctcccaag ccgaacgcgc
     2341 gcacaaggac gacgacgacg gcggccacgc caatggcgaa gcccaacgcg aaggcgaagg
     2401 cgaaacgaaa cgccaaagcc acgccgaagg ccgcggccaa gaccaagacg aagctgaaag
     2461 ccaacgcgaa ggcgaaggcg aaggtgaagg tgaaggtgaa ggtgaaggcg acggcgaagg
     2521 ccaagccggc caagcgaaga cgccgctagc tcgggcgaag tggtcgcgtg cgcgggacgc
     2581 ggcagcgtca agaaatgtcg gatacccggt tccgccgaag ccgagctgat tcgacgtaga
     2641 atgggtatgt ggccgatcgt cctgacggat cgtgggatgc ccccacggaa cacgacgatc
     2701 agcacgagat cgattctggt gagttcgggg ccgaagaccc gccgaccgta aaagccaccg
     2761 agagccagcg cgcgcgtgcc cgcggtgacg cgatcgcgaa ccagctcgcg agcgtgaacc
     2821 gcaacgtcgt tgccaagccc gctgcgccgt tgcctgcgcc gccgatgcca ctaccgtcga
     2881 ccacgctgcc gccgccagtc gtgcagcgcg cgcctgccaa gccgagcacg cctagcgaca
     2941 gcatcgtcat gctcgacaag gtgtccagca aggcggcggc cgagcccgca ccgcggcgcg
     3001 gcgagtcggc agcgggtggg attgtcgtgg tcgcgaccgg cggcgatgcc gaacgcatcc
     3061 gcaagctctg ccacaagcac ggcctgctcg tgccggtgat cggatcgctt gcagccgtcc
     3121 acgttgcggt gtcgatcgtc gtcgtcggcg aaccgtcgcc acccgcgccc gagcgtgtcg
     3181 tccacgtcgt gcggccgacc gtgcccgacg acaagctcgt cgatctgctg cgcgcgctcg
     3241 tcagcggccg cgtgattgtc gagccgcccg aggcggtcgt gccgatcaac tcgcgtgtcg
     3301 gcgaggcagt gcggcgcctc gcgacgctct ccgatcgcgg tgcgatcgag atgacgacga
     3361 tcgaagcgat caccgcgatc acggcgtgcg atcgcgcgca ctgcctgttc tacgatccat
     3421 cgacgagtgc gctgtggtcg gaggcgaagc gccgcgaagg aagcgacgat cgccaggcga
     3481 tgggcggcat cgtcggctgg gcggcgctta ccgggcagac gatttacgca tcgccggccg
     3541 gcgacgatcc acgctggctg caggaagtcg atgatccgga cggcaagccg cagtcgcgcg
     3601 tgctcgtgca accgatcatc ggggccgatc gccgcgtgca cgccgtcctg atcgcaatcc
     3661 ggcgctggcg acacgccgac ttcagcgatg tggagcgcaa tgcgctcgcg tcgttcgcag
     3721 cacttgccgg ccccgcactc gatctggcgg tcgcggcgtc gcctccaccc acgccacgca
     3781 aggcgcgctc gacgctcccc ggcgcggcag cctcgaagtc cgcgatcgtg tcgacaccgc
     3841 gcgcggcgag cgagtcacgg ccgccgcccg tcaccgcgcg ctccggccca gaggccgggc
     3901 cgcccggatc gatgacacgg ggccgcgaca acgcgcgcag ccacaagccg tcgacgcaac
     3961 cgccgccgac cgtcgagctg ccaccgccgt ccgacgagtt cacgagcgtc gacgacaacg
     4021 atcgctactc gagcgattcg attctctcgc ttccctcgcc tccccacctc cccgcgccga
     4081 tcgatcagcc gccggtgccg tacaagacgg tcaaacccgc ggcgcgcaag cgcccgcgca
     4141 cgcactccga ttcgcaagcg ccaaagccgc gtggcgatgg tgacctcgtc gatctcgcgg
     4201 tcgtcgcgat cgatgacgac gcgcgccgcg tgcagcgcct cgtcaagaag acgcgattcg
     4261 agatctcgac gttcgcgcgg ctcgccgatg tgccggactt ctatcgcgtc ctcacgctcg
     4321 gcgacgcctg gcaaggcgac gacgcgcgca tcatctacgc ggcacgctcg tcgatccccg
     4381 acgatcagct caacgatctg ttcgatgcgc tcgtccacga tcgcgtcgtc gcccccgccg
     4441 gtccgctgac ggctccgcag agcatcgcgg aagcgcgtcg ctctcagatc gcgcttgcgg
     4501 gcgcgcgcaa gctcgccgtg gccgtagacc tcgcagccgc cgagacgatc accgtcgaga
     4561 cgatgcgcga gctcctcgat accgaccgcg cctactgctg gatcttcgag ccggacgcgg
     4621 gtctgatgtg gtcagaggcg cgccgccgcg tcggcatcga gccgttccgc gcgatcgcag
     4681 gcatcacagg gtattgcgcg cgcaccggca aggccgcgaa cgtgccccac gcgagcgctg
     4741 atccgcgctg gctcgctccg ctcgacgacc ccgacggcga tccgcacagc cagctgctcg
     4801 tccaaccggt cgtgcgcaac gacggccgcg tacacggcct cttggttgct gtccgccggc
     4861 cgcgccgccc gggcttcacc gatctcgacg tcacactggc cgctcgcttc gccggcctcg
     4921 tcgcgccgct gttcgagcag atcgcggtca cgcaaacgtc gcaggagctc gtcggcgaag
     4981 acacctccgc gcggccgccg atcgagccgc gcgctcagtc gtcggtcagc atgatgaccc
     5041 gcgtcgagaa tccgatcatt cgctgggtct tcctcgcgat cggattggcc gtcggcgtga
     5101 tcattggcgt gatgctctag gcgtccgccc agttcgtgcc gatgccgatg tcgacttcga
     5161 gcggcacgct cagcttgtag acgttttcca tctcgtcttt gagcgcggcg ccgacggtgt
     5221 cggcgatgtc gggcggcgac tcgaacacga gctcgtcgtg cacggtgagc agcatctgca
     5281 ccggccagcc ttcgcgcgcg atccgttcgg tcgtgcgcag catcgcgagc ttgatgatgt
     5341 ccgcgccggt gccctgcatc ggcgtgttct gggcgacgcg ctcggcggcg tgtcgtgcgg
     5401 ccgggctctt cgacatcaga tttggaatcg gccgccaacg cccgagcacg gtgcgcgcac
     5461 caccttgcgc acgcgcgacc gcgacgatct cgtccatgaa cttgtggatc gtcgggaagc
     5521 gctggaagta gcggcgcgaa tactcggcgg cttgcgtgcg cgtgacgtcg agggcgcgcg
     5581 cgaggccgaa atcggactgg ccgtacgaca ggccatagtt gacggccttc gcgacgcggc
     5641 gctgctcggc ggtgacttgc tcgcgcggca cgccgaacac ctcggccgcc gtctgcgtgt
     5701 ggacgtcgac gtgatcacga aatgccgatg acagcagcgg atcgcctgac aggtgcgcca
     5761 ggatgcgcag ctcgatctgc gagtagtccg ccgcgatgag cacgtggccc ggcgctgcga
     5821 tgaaaccgcg gcggatctgc atgccgaggt cgctgcggat cggaatgttc tgaagatttg
     5881 gatcttgcga cgagatgcgc ccggtttcgg cgacgacctg gttgaacgtg gtgtgcacgc
     5941 gaccggtctt cgacgatacg agcggcggca gcgcatcgag gtacgtgcct ttgagcttgc
     6001 tgatctcgcg atgctcgagg atcggcttga tgatcggatg ctcgagctgc tcgagcgtgt
     6061 cggcctccgt cgaccagccg gtcttcgtgc gccgcacgcc cttggtcgcg aggccgagct
     6121 tctcgaacaa cagctcgccg agctgcttcg gcgaactgac gttgagctct tcgccggcga
     6181 tctcgtagat ctgtttctcg agaccttcga ggcgcgccga cacctcggcg ccgagcttct
     6241 tgaagtgatt cgtgtcgatg tgaatgccga cacgctcgat gcgcgcgagc agattcgcga
     6301 ccggcagctc gatctcgcgg tacagcgccg cgagcccgct cgcatcgaga cgcgcgggca
     6361 gctcgtcggc gatcggcaac agcgagtacg tgatcgcacc agtccatggc gcggcacgct
     6421 cgacctcgac cgcttcgaga ctcttcgcgc gaccgacgat cttgtcgcga ccgggaagca
     6481 gcacaccacc gagccgctgc gcgaccgcct cgcctggctc ggcatccgcc gtcggatcga
     6541 gcaagaacgc ggcgagcatc gtgtcctcga cgatgccggc gaccgtgacg ccgatgtcga
     6601 ggaacgcgcg cacgacttgc tttgcgttgt ggcaaatctt cgccaccgtc ggatcttcga
     6661 gcacgcggtg cagcggcacg agatcgccgg cgaccggcgg cgacggtgcg ccgatgtagc
     6721 gatgcgcgag cggaatgtag gcgggcgcct ggccgggcac ggcgacgatc acggagacca
     6781 tgcgcgcacg ctcgatacgc tcggggtcga gctcgacggt gatcgcgagc ttgcccgctg
     6841 cctcggcatg cttcgcgagc tcggcgatcg cgtcgccgcg cacgatgacc ttcgcgtcct
     6901 gacgtgcttc aacggtcgcg agtggcgtcg cttcgggcgc ggggacgacg atcatgtcct
     6961 cgcccggcgg catcttcatc ttcaccttct cgacgagcac ctggaactcg agctcggtga
     7021 acagctggag cagtacgggc aggtcccact gaccgacgac cagctcttcg agcggttgca
     7081 gcggtacgtc gcgcttgagc tcgacgagct ggcgcgaaat cttcacgcgc tcgagcatct
     7141 cgggatcgcc gaacggctgc ttgaccgaga gccgcggcac gaccgggttc gtcttgatca
     7201 gattgtcgag cgtgccgtac tgctcgagga gattcgcggc ggtcttgtcg ccgacgccgc
     7261 gaaggcccgg gatgttgtcg ctggtgtcgc cgacgagcgc gaggaagtcc ggaatcttct
     7321 ccggcggcac gcccatcttc ttgatcactt cttcgcgcgt gtacgtcttc tgatggagcg
     7381 cgtcgatcat cgtcacgcca tcgccgatca actgcatgat gtctttgtcg gcgctgaaga
     7441 tgacgacttc ccagcccttc ttcttcgcct ccaccacgag cgtcgcgatc acgtcgtcgg
     7501 cttcgacacc ggggaccgcg aggcagggcc acccgaggcc gccgacgacc ttcgggaagt
     7561 actccatctg cacttgcagc tcttcgggag tctctttgcg gtgcgccttg taatccgcgt
     7621 acatatcggc gcggaacgtc ttcttgcccg atttgtcgtc gaatacgacc gccatccgct
     7681 cgggtttgaa gtcctcctcg agccgcatca acatccgcga aaacacgtag agcgcaccgg
     7741 tgggcatgcc ctcggacgtc gacagccgga cctcgcgacg ctcgccacgc gagacgttca
     7801 tcaggccgta atgggcgcgg taaatgtacc catgcccgtc gacgatatag agccgctccg
     7861 ccatcttgcg ggggcactct agcccatcta ttcgcccggg cacccagtcg aaaagcgagt
     7921 tgttcgcacc cgatctggcg tacgatcggg ccgtgcgcgc agcagccgta gccctgctcg
     7981 tttccgcctg ctcgttcaac catggcctcg gcgcgttcga tgcgtctggc ggcggcggtg
     8041 acgacaccgg tagcggtagc ggcagcggca gcgacgcgat gatgggctcc gacggcggtg
     8101 gcagcgggag cgacgggccg atgtcgacgc tgcgccagaa gacgatcacg atcggcacga
     8161 tcgcaggcgg tccgcacgcc gattttccgg tctggatcac gctgagcgac gcggatctca
     8221 tggcccgcgc gcgcaccgac ggcacggaca tccacttctt gatcggcggg cagcccgccg
     8281 actaccagat ccagagctgg gtcaagaata ccggccggct cgacgcatgg gtgcgcgtgc
     8341 cgagcctcga taccgggacg cagatcgtga tgcgttacgg cgacgtcagc gtcgcgcaca
     8401 cgcccgacgt accgggcacg ttcggcggtt accaagcggt gtggcatctc gacgatgcgc
     8461 tcaacaacac gacggtcgcc gacgcgcgca acctgcataa cggcacggcg gtgatgctac
     8521 aggcgaacga cagcacggcg gcgcagctcg gtaacggtat caatttcagc gacggcaacg
     8581 atcagatcac gttcacgaac ccgctggccg gcaacagcgc gcatacgatc tcgttctgga
     8641 tcaaccagcg ggccactgcg tcgaacgact gcgtgatcgc gatgggcaac gggcagttga
     8701 acgaggcgcg ctggtttcac agccgctaca acgcgagcac ggtcgccgtc ggcttctata
     8761 cgaacgactg ggacaatccg gccgagaaca tcatcggcgg caactggacg ctcatgcact
     8821 gggtctacga gggcaaccgc atgtcccgca tctaccgcaa tggcgtgctc gtcgcgggac
     8881 cgttcatgca ccaaaacaac atcgacacga tgggtaccgc cggctcgatc ggcaacgcgc
     8941 cggtcgccta tggcgcgaac atggggctca acgccgcgct cgatgagatg cgcatcatca
     9001 acgtcgtgcg caacgcgaac tggatcgcca tggagtacaa gaaccagaag acgccgagca
     9061 tgttctacac ggttggcccc gagatgcagt aggccgcggt gcacgcctga acgcggcttc
     9121 gtgttaagaa tccccaatgc accgcatcgt cgtcgacgcg atgggaggcg atcacgcgcc
     9181 ggacgaggtc gtgcggggtg ccgccgaggc gtcgctcgcg ctgaccaacg ccgagatcat
     9241 cctggtcggt gatgccgcgg tgctcggccg cctactcccg cggatgcgcc acgatggcgc
     9301 gcgcgtgcgc gtacaccacg ctccggcatt cgtcgagatg gacgaaaagc ccgccgaggc
     9361 gctcgcggcc aagcccgagg catcgatcgc cgtcgccgcg gacctggtcg cacgtggtga
     9421 aggcgacgcc ctggtatcgg caggcaacac cggcgcgtcg gtactcgcat gcgctcggcg
     9481 ttggacgctg ctcaccggcg tacggcgtgc ggcgctcgca gccgtctatc cgacggagct
     9541 acgccgcggc gaaaaagacg atccgttttc gctgatcctc gatgtcggcg cgacgatcga
     9601 cgcgaccgcc gaggatctcg ttggcttcgc cgtgatgggc tcggcgtacg cgaagctgat
     9661 ctcgcagaat cgccgcccgc gcgtcgcgct gctctcgaac ggtaccgagg ccggcaaagg
     9721 tccacccgag gtcgtgcgtg cccacgagca gctcgtcgag acgaccgagc tgaacttcat
     9781 cggcaacatc gagggtctcg acattccgcg cggcgtcgcc gacgtcgtcg tgacgtcggg
     9841 cttcgtcggc aacgtcgtgc tcaagatgct cgaaggtgtg agcgagacgg tcgtgcggct
     9901 cgcgcgctat gcgcacaagg agcggctcgc gtggcgcctc gggctcgtcg cgctgtcgag
     9961 cgcgatcgat cagctcaagg aagtcaccga ctggcagcag tacggcggcg cgccactgct
    10021 cggcttcacg catccgttca tcaaggcaca tggccgctcg aatgcgcgcg cagtgcagaa
    10081 cgcggtgaag gtcgcccaca aagctctcgc cggaaatctg tgcggcaaca tcgcgcgcac
    10141 gatggcggag ctcgatgaac gtgcgcagcg ccgcacgacg agtcaaggtg acgtggttgg
    10201 tgcgtagtgt tccggagaat ttcgaagcta cctgtccgta ttggtgaacg catcggcttg
    10261 ttcgagccgg cgacacctca gctcgcgccc ggcgagcggt tgccgaagcg cacgttccgc
    10321 tgggacctcg acaagacgta tctgcgtacc gagttcgact cgctgaagga tctcgcgaag
    10381 agcgcgatcg agaccgcggc tgacaagcag gcgtatcccg gcgcgaccgc gctactgcgt
    10441 gcgctgcgcc aggacggcaa tcggatctgc atcgtatcgg gaagcccgac gcagatgcgt
    10501 caggtgctcg ccgccaagct cgcgctcgac ggcgtcgagt acgacgagtt cgtgctcaag
    10561 aacaatctga agaacatcct gcgtggccgc ttccgtgcgt tgcgcgcgca gattccatac
    10621 aagctgccgg cgatgctctc gtcacgcatc aacgtcccgg cgccgagcga gatgttgttc
    10681 ggcgacgacg ccgaagccga cgcgatcatc tactgcctgt atgccgatct gatcgccggc
    10741 gaggtctcgc tgacggatct cgagcgcgtg ctgatcgcgt cacgcgcgta cgacgacgac
    10801 gccgagcggt gcctcgatct cgcgcgccgc gtgccgcaag cgaaggccgt cacgcggatg
    10861 ttcattcatc tcgatcgccg ttcaccgccg atgggcttcc gtcggttcgg cccgcgcctc
    10921 gtccccgtgt tcaactattt ccaggccgcg ctcgtgctct acgaagacca ggtgctgacc
    10981 gcgcgccagg tgatcttcgt cgcactcgag atgctcgatt ccggacagtt tgcgctcggt
    11041 cacctcgcga cgagtgtgca ggatgtgatt cgtcgcggac gagtcacgcg tgaatcggcg
    11101 ttgcggctta ctgaagaagc agccgaggcg gcacgttcgg gcgcgctctc gggacgaagc
    11161 gatctgccgc cgttcgaaaa aatcgcagag tcctttcgcg agcgagtccg agagcttggg
    11221 ggaacggttg cccccgagcc cgcggttgcc gaaccgctcg actacgtgcg cttggtcgac
    11281 gaggagcacg aacaccgaca ccgtcgccgt cgccaaggca tgggctgaga cgccccgtga
    11341 cagctgtacc gtctcggcga gatgcacgcc tcgcatatcg attaacccct gtgagcccaa
    11401 agtttcgcgc cggtgccgga cgttttgtac gttgaatctg tgcgtctctg cattgctgcc
    11461 gtagtcctcg ccgcgtgcgg cggtggtggc ggcacacagg cgccagacgc atcacctccc
    11521 gacgcgccga tcgacatgcc gccgatgttc gccgcgtgtc gcgagttcgg cggcagcacg
    11581 gcaaccgtcc ccgcgcacgt gacgggctcg ctcgccggtc ccgacgtgca gagcccgtcg
    11641 cagtgcacgg cggtcgatgc gccgtacggc atcgagtctg cgggccccga cgcggttgtc
    11701 cggatcgacg gtctggttcc cggcaccgcg tacatcgtgc agctcgactc gcccgaggat
    11761 ctcgcgttct acgtcgtgag cggctgctcg acgccatcgg gaccggcggc gaacgagtgc
    11821 tcgttgttcg tcgacgcgag tgccggctcg gaagaagtcg gccgcttcgt ggcggcgtcg
    11881 acgacgacgt atatcgtggt cgactactac gcgtcgcaca cgccggcgaa ccagacgttc
    11941 gcgctcgatg tctacgccga ggcgtgcacg accaactcgc agtgcagcgc aggcgcgccg
    12001 gtgtgctcga acggcaagtg cgtgcagtgt gcgtcgagct tcgattgcgt cgactcgcag
    12061 gcgccggtct gcgatcgcac agcccaaacg tgcggcccgg gctcggacat gtgcctgagc
    12121 gacgacagcg tcgagcctgc cgacgatggg cccgccggtg cgcgtgcgct cgtgccgaac
    12181 ggcggcgggt tcgcgtcggc gaccggcgag atctgcagca cgccgcgcag cgaggcagac
    12241 ttctattcgt tccaggtcac ctcggtcggc gaggtctggg acttctcact gctgtgggcg
    12301 ggcacggacg atctcgacct cgagatcttc gacgcgaatg gctcggcgta cggactgtcg
    12361 tattgggagc agccggaagc cgcgcggctc acgtacctgc cgaccggcac gtacttcgtg
    12421 aagatcagcg acttcacgag ccagaccacg acgccggtcg cgtacacgct cagcgcgcga
    12481 cgcacggcgg gtcccggctg cacgacccgc gcagactgcg cgagcgagta cagaaaccag
    12541 atcttccgcg gtgactgcgt ggccggcgcg tgcgtgaaga tcgagggcgc cggcttggtc
    12601 gaggaaggcg gcgcgtgcga tagcgtctcg gactgcggca gcatgctgtc ctgcccgagc
    12661 ttcttcttcg tcgcgaacgc cgatacccgc gacgtctgcg cgcgcacgtg cagcggtgac
    12721 gccgactgcg ccgcgctggg cgaaagctat gtctgcacga cgtatctgat ccggaatttc
    12781 tgcgtccaga agtgcaccac caacgagcaa tgcgcgacgt cgctggactc gacgccgatg
    12841 tccggtccct ggtatcggct gacttgcacg cccgcgaccg gtcgctgcgc cccgtagctg
    12901 ctatataacc ctcgcatgga ccgaagcggc tcgatcctgg tcgtcgaaga cgatgcggcg
    12961 atgcgcgagc tgctgaccga agagctggcc gatgccggct tcacggtgca gtcggcgggt
    13021 agcgcggccg gcgggctcga gatcgcgcgc agcggtcgct tcgagctcgt ggtcaccgat
    13081 ctgcgcatgc ccgagatgga cggcttcgac ctgatccgcg gcgtgatggc gctccccgag
    13141 ccgccgcaca tcatcatgat caccgcgttc ggctcgatcg agactgccat tcgcgcggtc
    13201 aagctcggcg cctacgacta catcaccaag ccgttcgaga tcgaagagct gctgctcgtc
    13261 gctgacaagg cactcggcga acgcgcgctg cgcagcaagg tcgcgcggct gcagcgcgag
    13321 gtcgagaagg agtatggcct cggcggcatc atcgcgaaga gccagtcgat gcgcgatgtc
    13381 atctccgatg tgcgtcggat cgccggctcg accgcgtcgg tgctgatcac cggcgagagc
    13441 ggcaccggca aggagctgat cgcgcgcgcg atccactaca actcgtcgcg cgccgcgggc
    13501 ccgttcgtcg gcgtgaacct cgcggccgtg cccgaaggcc tgatcgagag cgagctggtc
    13561 ggtcacaaga cgggcgcgtt caccgacgcg cgcaacgaca agcccggcct gttcgtcgag
    13621 gccgacgcgg gcacgatctt cctcgacgag atcggcgagc tcgcgctgcc gttgcaggcc
    13681 aagctcttgc gtgtgctgca agagcacgag gtgcggccgc tcggcgcgac gaagaacacg
    13741 cgcatcgacg tccgcgttgt cgcggcaacg aacaagaacc ttggcgcgat gctcgccgac
    13801 ggcagcttcc gcgaagatct ctactaccgg atcaacgtca tcaacctcga tctaccgccg
    13861 ctgcgctcgc gccccgagga catcatcccg ctcgccgagc acgtgctcgc gcaactcggc
    13921 gccaagcaac agccacagcg ccgcatgcgg ctgtcgcccg aagcgcagca cctgctgctc
    13981 gcgtatcact ggcctggcaa tgtgcgcgag ctgatgaacg tcctcgagcg aggcgtcgcg
    14041 ctgtgccagg gcgagctgat cgccgacgac gatctgccgc agcacgtgcg cgagcggcgg
    14101 cccgccgact tcctcggcgc tgcggtcgcg cgccggatga cgctgtccga gctcgagcgc
    14161 gaattcatcg agcgcgtgct cgaggacgaa gccggcaaca agacgcgcgc cgcacagcgg
    14221 ctcggtctcg atcgcaagac gctctatcgc aagctcgacg agtacgcgaa gcaagacggc
    14281 aaagccgagg caccgacgcg accgtcgcat cccgacaagc ccgacaaaga cgacgacgag
    14341 agttagcgtt agacggccga ccgcgcgcct gcgttacagc ggtgtaccga ggcctactga
    14401 ttgcaacccg ctctccgcga acccacgcga gagggctaat catgcaaaag ctacaactgt
    14461 gtgcggtagc gctcgcgctc gcagcgtgcg cttctgacac cgacgatccg agcaacgagc
    14521 cccgccgcgg taaggggcca cacacgccag agccgaccgt cgacgcggcg ttgacgccac
    14581 cgcccccgag cgatccggtg ggaaccgtca gttgctacac cgaaggcgcg ccacaggctg
    14641 cgtgcacgct gccgacgcac tgctgcttcg gcaactacag cgcgcagcac aatggctact
    14701 gcacgaccga ggcttgcgca gagagcacga tcgcgtgcga cggcccggag gattgcgcga
    14761 acggccagca ttgctgcgcg accctgctcg atatcggccg ctggaatctc gcctgccaga
    14821 gcagcgcctg cagcccgcag ccgctcggcg acgagctgtg ccacgacagc agcacctgcg
    14881 gcggcggcac gtgcgtgagc gcgctcgacg ccaactacga cttgccgcga acgctgtcgg
    14941 tctgccgcta gcgcgccgtc tcgcgcaagt actcgcggaa ccggcgcagt ccgacctccg
    15001 tcttgagatc ggtgagcgcg ccggcgcgac acgcggcaat cgcgtcgtcg aggatgagcc
    15061 acgtcgtgcg cgcaccctcc tccatcggcg agccatcgcc ggtaagcggc tgctgcgtcg
    15121 ccggatcgac ttcgaccgcg gtgaagtaga acttctcgac gaggcacccg ggcgacggaa
    15181 acatgccggc gccgagcagt acgaccttgt cttgctcgac gaggaagccg gcctcttcga
    15241 gcacctcgtg cgcggcgcgc gccttgagcc cgagctcgcc ttggtcgcct tcctcgacga
    15301 tgccggcgac gagctcggtc aagaacaagc cgggggcctg ctccggcaac ggcgcgcgcg
    15361 caggatcgcg accgagcacg agcgcggggc gcagcccgtg ccgcacgagc acctcggtgc
    15421 cacgttcggt ccgtgcgtag acgacgacga cgaccgcgtc ctggccgaac ggccgcacga
    15481 ccgcatcgca gatgtactgc gccgatgtgc tgccatcacc gtggacgttg cgcaggtgaa
    15541 gccggcgaat tgcgaggaag cctccgtcgc cgacgcgctc gtcgctgtcg acgatcagat
    15601 cacggacttc gctcacggtg cggcggaacc cgcagccgaa ccagcggccg gcggagctgc
    15661 cgcgctaccc gccgacggct cgtcaggcga cggaccgggc gcgggcggtg gcggcaggcc
    15721 accgagctgc ttgacctcgt acttcgcaag cgccgagtga atgtcgaccg cgagctgatc
    15781 gaccttgctc gtcgcccagc ccggcacgct cggatcgttc tcgggcaccg gatccggctt
    15841 tggcataccc gcgtggaagc gcagcatcac cggcgtcact ttgacgatcc agttgctgcc
    15901 gtcgggtagc agcgtgacga cgagcgtgat gttgacggag ccgtcgggga actgcgagtc
    15961 cgcgtccgtt cgcgtcattc cgccgcccat gtcgcgcgtc gacgttccgt taccaccctc
    16021 gcgcgtgacc gccatgcctt cgggcgtgaa ccaccttcct tcggtctgca taccgagtgt
    16081 ggtctcgtcc gacttctgca gcttgtactt cgattcgacg gccgccttgg tggcggcgaa
    16141 cagcgtgagc ttgtcgcctt gatagcgagc cgccttcgcg gtgccgactt ccttcgagga
    16201 cggaccgcac gccacggcgg cgaccgtcag tagcgcaacc aggtattgca tggcctggag
    16261 ttgactactg cacggcgtag ttctgcaagc gcttgtggat ctctagctgc aaggaatcta
    16321 cgcgtccagg cacccagcct ggcaggttcg gatcgtctgg ctttagctcg cgtggttgcg
    16381 gacttccgga caacgcctgg aaggtcttcg gcgtgaccgt gaccatgaag ccccccgcga
    16441 ccgagaccag ctccacgatg agctcgaggc gcacgctatg atctcgaatc tgggtgtagc
    16501 cctggtcacc ggcggattcg cgcccgccct cggcggtgta ccactgcggg acggtggcca
    16561 gcacgtagct gtcttcgtcc ccgccgttcg cgttggcaat cttgtaggtc tcctgggtca
    16621 cggcgatgac ttcgttgaac atctcgctcg cgttcccgcg gtagtgggcc gtcttcgcag
    16681 tcttgatctc ggccgccgac ggcccgcaag acatgcacag tgcaacaatt tgcagcagca
    16741 gcaaaactcg catggacagt gatagcgttc gcgcatgtcc gagctcaagg gcaaacgcgc
    16801 gctggtcacc ggtgcctcga gcggcatcgg cgccgagatc gctcacgagc tcgcacgccg
    16861 cggcgtgcat ctcgtcctcg ccgcacgccg gcggcagcac ctcgacgacg tggcgagcgc
    16921 gtgcgccgag catggcgtcg ctaccgaggt gatcaccgcg gacctcggca cggcgggcgg
    16981 cgcccgtgcc ctgtggtcga cggcaacgcg cacgccggtc gacatcctga tcaataatgc
    17041 cggcttcggt tacttccgcc cattcgccga gatcgattgg gcgcgtgacg ccgagctcgt
    17101 ccagctcaac atcacgtcgc tcgtcgagct gtcgaagctg ttccgcgagc accgtcaggg
    17161 gcgcgacggc cgcgcgtacc tcgtgaacat cgcgtcgatc gccgcgtatc agtcggtgcc
    17221 gaacatggcg ctgtacacgt cgtcgaaagc gttcgtccgc aacttcaccg aggcgctgca
    17281 cgacgagtgg aagggcacgc agctatcagc gacgtgcatc tgcccgggcg gcaccaagac
    17341 cgacttccac gcgatggccg gcgccggcga ttacggctgg ctcgcgaacg tctcgatgat
    17401 gacggccgag cgggtcgcgc ggatctcggt cgacgcgatg gcgcgcggcg cgcgcaacgt
    17461 gatccctggc ttcatgaaca agctctcgtg ctggggcgtg cggctggtgc cgcgccgatt
    17521 cgcgtcgtgg atgtcgcgcc gcgtgctcgg caaaccgcga cccggtgaat tgcccgcgcg
    17581 tacgacggag gccgcatgac cgagcgccat aacgttcgcc actggaccaa cgacacgcgc
    17641 ggcgtgtacg aggtctggta cctgacgtgg aaccacccgg cgacggacca gggcttctgg
    17701 cttcgctaca tcaccgaggc gcccgtcgac ggaccggcgc gcggggagct gtggttcgcg
    17761 cgcttcgatc cgaagaatcc ggcgcgcacg ttcggcatcc acaagcggtt tccgatcgat
    17821 cagctgcaat cggcagccga tccgttctcg ctgtcgatcg ccggcagccg gctcggtcac
    17881 gatcacgcga tcgggcagct cgccggcaac ggccacgacg tgcgctggga tctgcgctgg
    17941 gacgcggcgg ctcaggtgct gcgcctgctc cccgacgtga tgtacgcgcg cggcggactg
    18001 ggcgagacga cggtgcagtc gccgaatccg cgcgtcgcga tgaccggcac gctcgtcgtc
    18061 gacggcgaga agctcacgtt cgatcgcgcg acactcggcc agacccacct gtggggcaag
    18121 aagcacgcgt actcgtggac atggggacgc tgtgccgagt tcgcgggcgc gcccgacgcg
    18181 ctgctcgaga tcctcgcagt ccgcctgcag cggcgcggca ccacgctccc gccgatgacg
    18241 ctcgtaacgt tgaacctcga cggcgagcaa tatcggctga accagttccg tcacgtggtg
    18301 cgcaaccgcg gcacgtgggc aaacggccac gtcacgttct cgggatggtc gccgaccgtt
    18361 aggatcgaag gcgagctgtc gtgccggccg gaccagatgg tgtcggcgcc ctacctcgat
    18421 ccggacggca ccgaagtgtt ctgcgcgaat accgagatcg gcgacgcgcg cgtgacggtg
    18481 tttcggcgtt ccgggctgcg ctggcgggag caccgcacga tcgaaagcaa gggccatgcg
    18541 cacttcgagc tcggcgggcg tacgcgcgat cctgccgtcg agcgcgagca catgctcgtt
    18601 ccctgatgta gggttcgccc caatggagcc gagaaagacc ccgctctggg agacgcacaa
    18661 gaagctcggt gcgaagctgg tcgacttcgc cggctggcac atgccggtgt cctatccggc
    18721 cggcacgatc aaagagcaca aggccgtacg cgaagcgctc ggtctgttcg acgtctcgca
    18781 catgggcgag gcctacctgc ggggcccgcg tgcgcgcgag gtcgtcgcga agctcgcgac
    18841 caacgcatgc gaatcggcgc cccccggcaa agcggtctac acgctcctct gccggcccaa
    18901 cggcggcatc gtcgacgact gcatcttcta caagaaatcc gacgacgatt tcatggtcat
    18961 cttcaacgcg ggcaacgtcg ccaaggacct cgactggttc cgcgagcacg cgtcgaccac
    19021 ctgcgatgtc atcgacgtct ccgacgagac cgcgctgatc gcagtgcaag gaccgaaggc
    19081 ggttggcgtc gtcgacaagc tcggcgacgg caccctgtcg tcgatgccga gcttcacgtt
    19141 ccgcgacacg acgatcgcgg gcgtgaagtg catggccgcg cgcaccggct acaccggcga
    19201 ggacggcttc gagctcgcgt gcgcgaacgc cgacgccacg aagctctgga acgcactact
    19261 cgacgccgcg acgcctgcgg gcggcctgcc gatcggcctc ggcgcgcgcg actcgctgcg
    19321 cctcgaatcg aagctgccgc tgtacggcaa cgacctcgat gacgatcaca cgccgctgga
    19381 ggcgggcctc ggctgggcgg tcaagctcga cggccgcaac ttcctcggct cgcaggcgct
    19441 gcgcgatcag aaggcgaagg gcctcacccg ccagctcgtc ggcttcaaga tcgacgagag
    19501 tgtgcgtgcg atcgcgcgcc acggctatcc gattgtcgac cgtagcctcg ctgccgcgga
    19561 tcaagtcatc ggcaccgtga cgagcggcgg tcccggcatc tacgtaggcg gcgcgatcgg
    19621 tctcggctac gtcccaaccg cgctcgccgc ggcaggtacc aagctgacga tcgattgccg
    19681 cggcaaggac gcgacggcga cagtggtttc cggcaagttc tacaagcgac cccagtgagt
    19741 atccgatgag cacctttcca accgagctcc gttacaccaa cgaccacgag tggattcgcg
    19801 tccagggtac ttcctggcgc gtcggcatca cgcagtttgc cgtcgatgcg ctcggcgaca
    19861 tcacgctcgt cgatctgccg aaggaaggcg atcaggtcac gaagggccag cgctttggca
    19921 ccgtcgagag cgtgaagagc gtcagcgacc tttacgcccc ggtttcgggc cgagtggtcg
    19981 cggtcaactc cgcgctcaag gacgcccccg aggacgtcaa tgccgagccc tacggcaaag
    20041 gctggatgat cgaagtcgag ccgaacgaca agggcgagct cgacgagttg atgacggccg
    20101 acgcgtacac gcagcacgtc gcggcgcagt gatcctcacc ccggcgcccc ctcggcacta
    20161 cttctcgatg ggaaagccgc gcttcaccca ttcgtcgaag ccgccgtcga gcgccaccgc
    20221 gcgcggatag cccatcgcgc gcagcgtcgc ggccgcgagc gtcgagatcc ggccgaactg
    20281 gcagtaggtc aggatgtgag cgctcggatc gggcagctcc ttgtcgacac gcagctcgag
    20341 ctgaccgcgc ggcaggtgct gcgcgcccgg catgtggccg ttgttgaacg cttccttctc
    20401 gcgcacgtcg agcagcacga ggccatcgcc accgcttgca atgcggtcgc gaacgtcgtc
    20461 gatgctcatg aacgcgatgt cgcgcgatgc ctcggcgagc agctgcgaga cggttttacc
    20521 gccggagcgg ttcgtgcgca gcgcctcgct caggtgatcg ggcatcttca gatcgaggct
    20581 gtgcatcagc gcgacgaaat ccgcgcgctc cttcttctgc aggcgtgggt tcgacgcctt
    20641 ctcggcgcca atcgtcgacg acgagcggtc cttgtagtcg tgcgccgggt acaccttcat
    20701 cgagtcgggc agcttgagca gcttgccgaa caagctgtcg tagagcgcac tcgcgtcgcc
    20761 ggtcggcaag tctgtgcggc cggtgccgcc gatcaggagc gtatcgccgg tcagcacgcg
    20821 atcctcgaag acgaggcatg tcgaatcgtt cgtgtgcccg ggcgtgtgga gcaccttgac
    20881 gcgtagcttg cccacgatga gcgtctcacc gtcgtcgacg cgcatgtcgg cgtacggcgc
    20941 cgccgcgttg cggtgcatca cgacgtgaac accgagccgc ttgccgagct cacgcgccgc
    21001 cgagaagtga tcggcatgcg tgtgcgtctc gtagatgtag tggacgcgca tgccgcgctc
    21061 tgcgatcagc gcgagatagc gatcgagctg atcgaggtgc ggatcgacga ccatcgccgc
    21121 acacgaatcg tcacaaccga tcaggtaaga cagacatccg ccagaacgga tttgctcgaa
    21181 gagcatgcgc cactctagcg cttccgcctt cgcacgagga gcacgagcac gccaatcgcc
    21241 aggccgccgc ttgcgccacc cgcgctgcaa ttgcagcccg actccttctc cggactgcgc
    21301 gccagcgtcg tcgtcggcgg cggctcttta ccgatcgtca gatcgagctc gggcacatcc
    21361 tgagcgacct gatcggcaag ctgcagcgta ccgcgcggcg cgtatgcgag atcttgtgcg
    21421 acctggatgg cgcctttgcg tgcccagcga ccgcgcacgg ggttgctgca cgtcagcggc
    21481 ccgagccatc gattccggat cgcgtagcgc gcctgaaatg cgctgtacga cgaggcctgc
    21541 gcactctgct cgaccgcacc gtcgggcccg cgttgttcgc gaccgcccgc gatcggcggc
    21601 acctccttga acgtcaggtc gttcttgacg gacttgtcgt agcgtacgtg catgcgcgtg
    21661 agcacgaagt cgctgctgcc gtacgcggtc gggctggcgg ccgtcccgtc gagcacgtcg
    21721 ccgccgagcg cctgaaacgc ggcgtagtcc agcgtcgggc ccggacacgg ctcacaggtc
    21781 gtcgcatccc acgcgtactc ggtgatcacg gcacctggat gcttcgcgag cgtcgcgtcg
    21841 aacagcgccg tgtaaaacgc cgcgaaccca tcgcgcgcgt tctgctt
//
LOCUS       DASUFX010000616        11723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_516899,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000616 DASUFX010000000
VERSION     DASUFX010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516899"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFV99_24740"
     CDS             <1..397
                     /locus_tag="VFV99_24740"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062612.1"
                     /translation="SLYIVGGRGANGYLADVERGYIEGNGTYDGSVLLAGLSLGTPRA
                     GHALSLIGNQLYVIGGANAGGAVDSVEVATFGADASAPLTPFAPAPGVHLHDPRGFAT
                     CTVIANELYAIGGQNTTGAIGSVERAKLH"
     gene            complement(446..1996)
                     /locus_tag="VFV99_24745"
     CDS             complement(446..1996)
                     /locus_tag="VFV99_24745"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX5062613.1"
                     /translation="MTAPALMVQGTASSVGKSLLVTALCRFFRREGVRVVPFKAQNMA
                     LNSAVTPDGLEIGRAQAVQAEAAGLTPAVEMNPILIKPETDRCSQVVVMGKPIGSMTA
                     VEYHAHKPRLQGVIADCLATLKARYDLVLIEGAGSPAEINLKARDIVNMHVARVADAP
                     VLLVGDIDRGGVFASFVGTLELLEPDERERIAAFVVNKFRGDIALLQPGLDFLTERTR
                     KPVLGVVPYVQQLRIADEDSVSLEDRAGRRRPSRDQLDIVVVRLPRISNYDDVEALEH
                     SPGVVVRFAERPEAIRGADLVILPGSKNTVSDLAWLRATGIAQVIAERAVAHEPVLGV
                     CGGCQMLGETIDDPFGVESPEPRVAGLGLLPLRTRFERAKTTAQVVARTAASSFLADA
                     GTDVTGYEIHMGVVESTSPCASTFELLSRNGAAVQVMDGAIDATGSVVGTMLHGIFEN
                     DGFRAGVLARLRTRRGLDAPPTDAVSAKDAEYDRLEHALRSSIDCELLWKLARVRPAP
                     KTKSRRIV"
     gene            complement(1993..3066)
                     /locus_tag="VFV99_24750"
     CDS             complement(1993..3066)
                     /locus_tag="VFV99_24750"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5062614.1"
                     /translation="MSTPEHLAPRVHGGPQDAELRALQIDPATLLDFSVNTNPYGPCA
                     AMVDAIRETRIDRYPDPTGRAARETIARTMDVSPDELVLGNGAAELLWTLARALIRPG
                     TKVLMVEPTFCEFSAAAAACGAQLVEWRAAAERGFALELDAIAARVAAEQPAVVYLCT
                     PNTPTGAGCSARDVARWAAANPQLTLVLDQSFLSLSDRFADAEVAMPANVVRIRSLTK
                     DHAIPGVRVGYLIVAPSLAARVEDQRPAWMTSAMAQTAAIVACRERAFVDESRRKILD
                     DRDHLAQAVALLGYVTVPSSACFFLVHVGDARELRQRLLVRHRVLVRDCASFGLPEYI
                     RLASRPAADRARLVNALAEERAR"
     gene            complement(3053..3991)
                     /locus_tag="VFV99_24755"
     CDS             complement(3053..3991)
                     /locus_tag="VFV99_24755"
                     /inference="COORDINATES: protein motif:HMM:NF002281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX5062615.1"
                     /translation="MTSSDPSAWVLVAALGIDVLLGEPPNAIHPVVWMGKLTRLLERM
                     SPARGRFAQLVAGALIAVTVPCAFAIGTFLVLRELAAWPVAAFIASVLLLKSTFAIRG
                     LGRAAHVVRDALASRGLGAARQGLRSLCSRDPSTLDEPQLVAATVESVAENTSDSFVA
                     PLFYYALFGLPGAMAYRAVNTLDAMIGYRGRYEYLGKASARLDDALNFIPARLTAVLL
                     LAAGAVCRRDVRTGWHMLRRDGRTTESPNAGQPMATMAGLLRVQLEKVGHYRLGDAVE
                     PLAVAKIDDAWRIVMVCAVIAAGCVIVALGATHVYA"
     gene            complement(3988..4677)
                     /locus_tag="VFV99_24760"
     CDS             complement(3988..4677)
                     /locus_tag="VFV99_24760"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEX5062616.1"
                     /translation="MTTAAMTVQLAPRLLVVRFAEPQRVVSWAMLGGGLGRTRAVAWV
                     EVRNDELRPPVDPCELARTRLEAAGIADAVTLMTSRDLRPYVDLERTYRDVSCRCIAT
                     VGLGNALRAGDPPGPDGRIGTINVLCCVSVGLTDEALLEALALASEARSLAVREAEVA
                     SPISGLPASGTGTDCIVIAAPDTADPHRFAGKHTELGHVVGAAVHDAVRSGAEQWKCE
                     QAAARAMERNR"
     gene            complement(4800..6431)
                     /locus_tag="VFV99_24765"
     CDS             complement(4800..6431)
                     /locus_tag="VFV99_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062617.1"
                     /translation="MRPTIPTILVTTFVASTALFGCENDSKLDHMAGMATAPVARTVD
                     QPAKLEFFVMSKCPYGVQVEKAVAPVLAKLGGNVDFHLTFIGQKQGDELSSMHGPSEV
                     AGDIAQLCARDVAPDKYMNMIACQNEDPSHVDTNWDSCGKRAGIDTSAVKSCIDTKGK
                     QLLAASFDEAEKRGATGSPTMFLNGKPYSGGRKTNDFLRAICNSFDSKKPAECQTIPT
                     PVAVNAVFFSDARCAKCNIDGLEGQLSGLFEGLKVKKVDYMTPEGKALYAQLKQADAS
                     FKTLPAVLFDSSLDGDKDGKQQVARYLHPVGAYQALALGGNFDPTAEICDNKVDDDGN
                     KQSDCDDAACKDAMACRPEIKKSLDLFVMSHCPYGTKAMLAAKEFSDAFGKDAAVNVH
                     FIGDDKGGQLSSMHGPEEVTDDLREICAVQHYGKDEKFLDFMACRSKDLKADWKACTG
                     SNGIDADVIAKCVDGEEGKGLLAASFKVASALDIQASPTFLVNNRETFNAQDAAGIAS
                     SFCKANPGLPGCAKQLSAAAPAQAAGGGGAACGTP"
     gene            6849..8387
                     /locus_tag="VFV99_24770"
     CDS             6849..8387
                     /locus_tag="VFV99_24770"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5062618.1"
                     /translation="MRTAAVESPIWAKRNSAPLSSLCVGSMLAALLAGGCATSVAPDD
                     PVQDWNDAMNLFGEGAQTVTPSDYGAGATPQNPPPPFHTTANVTCKPIVNGTSSVVVN
                     GRTRKFEVGLPANLSSKAAVLFVWHGFLQDSANFTNTVVYDPQAGAWKPFDTNAFNIP
                     LITITPVDSNLIPVWGLDWDIVSGEKDFPYFEAMLTCVEAQFQVDTTRVYSFGFSAGA
                     VFTNLLAAAYPHLFAATISESGTWFNDKAEWSEITVPLIGTTFMKWKWPALTAADGGA
                     VLMTHGGPNDFATVISLESSNEKALNFLYAGGRDVTECTHLFGHTLEPDLTQAMYYSF
                     MWQHQLGTRPLTLAAGLPTAQNPVFDTRCNFRPVVTPAAPGINNVVNAGFESALSNWA
                     YTGGVGFDNTTFARTGSGNGWVRANGGWNDVHQIFSVVPNRTYTVSGWIRTSDNNTDG
                     YFGVRSVAGDVVGEQKFGRFAGYTKVSVTVSSGSNTNLVVYGGLWANGDTFMQLDDVS
                     VVQQ"
     gene            complement(8447..8791)
                     /locus_tag="VFV99_24775"
     CDS             complement(8447..8791)
                     /locus_tag="VFV99_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062619.1"
                     /translation="MRHLIALILVGSLISGCSFFMTRAPAKDPGTRPLDCEKSMVPPG
                     ADAVGAGALTLIGLGSIATKSDDTSTTTFVVAELALFGVVAVLAYSAYSGHKSVKRCR
                     DLNALPATPVRE"
     gene            8909..9277
                     /locus_tag="VFV99_24780"
     CDS             8909..9277
                     /locus_tag="VFV99_24780"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5062620.1"
                     /translation="MKYLSFIRHSESYREATPPPALMEAMGKFIEKCRADGTLVDTGG
                     LLPSKEGVRIRSSRGKLTVKDGPFTESKEIIGGWAILQGSREDVLRVATEFMELHVRY
                     WPELECESEVRPMFDPGVGP"
     gene            9533..10222
                     /locus_tag="VFV99_24785"
     CDS             9533..10222
                     /locus_tag="VFV99_24785"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5062621.1"
                     /translation="MWSAPEVDVQAPIVAVDEVATDPAVEDTWTLGAESIERIGYRFG
                     HRHVVEVVRLGPLAIEVEIRTARAFLAMRAAAADAGVELGLASGFRTAEEQRELYRAW
                     RKGVGNKAARPGRSNHQSGRALDIAGVGAPGALAWLEANAASFGFTRTVKNEPWHWEY
                     VDIPIARPVVARIKRTKVGHPMRPAKRSNAIATTRQSSSKRVAASSQPDGHASKRNRR
                     MPTNMNSAAKP"
     gene            complement(10162..11244)
                     /locus_tag="VFV99_24790"
     CDS             complement(10162..11244)
                     /locus_tag="VFV99_24790"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5062622.1"
                     /translation="MFLATVKKELQLLRRDPRALVRLLALPVAFIALFGMVFRHAEHG
                     PTGRPLAIYVAPDSQLGATVVAALSHSTMFRPVALASAEEVRAKVAAEAFDAGLILPR
                     DFDPAHGGAKAELVIDTDKALAERGPIQGAVVAIVDAVVMGPRAETVAIVSPRQQPHE
                     TPDSFQMTVPGNAVLFGFFIALTCALSFAEERRTGTWRRLLAAPVSRWKLLTAKLVPY
                     VALGTLQVLLLFAIGVFGFGMQINGSIAALAVLTVGVAASATGLGLLLASVSSTEKQI
                     SSLGSVAILIMGLVGGCMLPRVLMPHLMQQIGLFVPHGWALDGYNALLVRHGTGFADV
                     IKPIAAVYGFAALFMFVGIRRFRFDA"
     gene            complement(11244..>11723)
                     /locus_tag="VFV99_24795"
     CDS             complement(11244..>11723)
                     /locus_tag="VFV99_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5062623.1"
                     /translation="LHKPTLVVLDEPTVGVDPQSRRHIFDSIRALRSQGMTVVYTSHY
                     MEEVEELCDRIAIMDAGAIVASGSLAELAAAHGAKGIELELDGDPTALDAAAKAVAEL
                     GGSRDGDVVRFAQLDALAPIVTAIEATGARIKRVGSGRADLETVFLALTGRGLRDAS"
BASE COUNT         2074 a         3874 c         3831 g         1944 t
ORIGIN      
        1 gtccctctac atcgtgggtg ggcgcggtgc gaacggctac cttgctgacg tcgagcgcgg
       61 ctacatcgaa ggcaacggca cgtacgacgg ctccgtgctg ctcgctgggc tctcgctcgg
      121 cacgcctcgc gcaggccacg cgctatcgct gatcggtaac cagctctatg tgatcggcgg
      181 cgcgaatgcc ggtggcgcgg tcgacagcgt cgaggttgcg acgttcggcg ccgacgcgtc
      241 cgcaccgctc acaccgttcg caccggcacc cggcgtccac ctccacgatc ctcgtggctt
      301 cgcgacctgt acggtgatcg cgaacgagct ctacgctatc ggcggccaga acacgaccgg
      361 agcgatcggc agcgtcgagc gcgcgaagct acattaacct tggtccgatc gtgagatagg
      421 ggcgggcggg tcgctcgggt atcccctaca cgatcctgcg acttttcgtt ttcggcgctg
      481 gtcgcacgcg tgcgagcttc catagcagct cgcagtcgat gctcgaacgc agcgcgtgct
      541 cgagccgatc atactcggcg tccttggcag agaccgcatc ggtcggcggc gcgtcaagac
      601 cgcgacgcgt gcggagtcgc gcgaggacac cagcgcggaa gccatcgttc tcgaaaatgc
      661 cgtgcagcat cgtgccgacg accgacccag tcgcgtcgat cgcgccgtcc atgacctgca
      721 cggcagcgcc attgcgcgag agcagctcga acgtgctcgc gcatggcgat gtcgactcga
      781 cgacgcccat gtggatctcg tagccggtca cgtcggtccc cgcatccgcg aggaacgagc
      841 tggctgcggt ccgagcgacg acctgggccg tggtcttggc gcgttcgaag cgggtgcgca
      901 gcggcaatag gccgagaccg gcgacgcgcg gctccggcga ttcgacgccg aacggatcat
      961 cgatcgtttc gccgagcatc tggcaaccgc cgcacacgcc gaggacgggc tcatgcgcga
     1021 ccgcacgttc tgcgatgacc tgcgcgatgc cggtcgctcg caaccacgcg aggtccgaca
     1081 cggtgttctt cgagcccggc aggatgacga ggtctgcacc gcgaatggcc tccgggcgct
     1141 ccgcgaaccg cacgacgacg ccgggggaat gctcgagcgc ttcgacatcg tcgtagttcg
     1201 agatccgcgg caatcggacg acgacgatat cgagctgatc gcgactgggc cgccgtcgac
     1261 ccgcgcgatc ctcgagtgac accgagtctt cgtcggcgat gcgtagttgc tgcacgtacg
     1321 gtacgacgcc gagcacgggc tttcgcgtcc gttcggtcaa gaaatcgagt cccggttgca
     1381 gcagcgcgat gtcgccgcgg aacttgttga cgacgaacgc cgcgatccgc tcgcgctcgt
     1441 ctggctcgag cagctcgagc gtgccgacga acgacgcgaa cacaccaccg cgatcgatat
     1501 cgccgacgag cagcaccggc gcatccgcga cgcgcgcgac gtgcatgttg acgatgtcgc
     1561 gcgccttcaa gttgatctcg gcggggcttc cggcgccttc gatcagcacg aggtcgtagc
     1621 gggccttgag tgtcgccaag cagtccgcga tcacgccctg cagccgcggc ttgtgcgcgt
     1681 ggtactcgac cgcggtcatg ctgccgatcg gcttgcccat gacgacgacc tgcgagcagc
     1741 gatcggtttc gggcttgatc aggatcgggt tcatctcgac ggcaggagtc aggcccgccg
     1801 cttcggcttg caccgcctgt gcacgcccga tctcgaggcc gtcgggcgtc accgccgagt
     1861 tcagcgccat gttctgcgcc ttgaacggca cgacgcggac cccttcgcgg cgaaagaacc
     1921 gacacagcgc cgtgacgagc aagctcttgc ccacggacga cgccgtgcct tgcaccatga
     1981 gcgcgggagc cgtcatcgcg cgcgttcctc ggcgagcgcg ttcaccagtc gcgctcgatc
     2041 cgccgcggga cgcgacgcga gccggatgta ctcgggcagt ccgaacgacg cgcagtctcg
     2101 aacgagcaca cggtgtcgca cgagcagccg ctggcgcagc tcgcgcgcgt ctccgacgtg
     2161 aacgagaaag aagcacgcgc tcgacgggac ggtgacatag ccgagcagag cgacggcttg
     2221 cgccaggtga tcgcggtcgt cgaggatctt gcggcggctc tcgtcgacga atgcgcgctc
     2281 gcggcacgcg acgatcgcgg cagtctgcgc catcgcactc gtcatccacg ctggacgctg
     2341 gtcttcgacg cgtgcggcga gcgacggcgc cacgatcaga tagccgacgc ggacacccgg
     2401 gatcgcgtgg tccttcgtga gcgagcgaat gcgaacgacg ttcgccggca tcgcgacctc
     2461 ggcatcggcg aaccggtcgc tgagcgacaa gaacgactga tcgagcacga gcgtgagctg
     2521 cgggttggcg gcggcccatc gcgcgacatc acgggccgag caaccagcac ccgtcggcgt
     2581 attcggcgtg cacagataaa cgaccgcggg ctgctcggcg gcgacgcgtg cagcgatcgc
     2641 atcgagctcg agcgcgaagc cacgctcggc ggccgcgcgc cactcgacga gctgcgctcc
     2701 acaagcagca gccgcggcgc tgaattcgca gaacgtcggc tcgaccatca acaccttggt
     2761 gccggggcgg atcagcgcac gggcgagcgt ccacagcagc tcggcggcgc cgttgccgag
     2821 cacgagctcg tcgggtgaca cgtccatcgt gcgcgcgatc gtctcgcgtg cagcgcggcc
     2881 agtcggatcg gggtagcgat cgatgcgcgt ctcgcggatc gcgtcgacca tcgcggcgca
     2941 cggaccgtac ggattcgtgt tgacgctgaa gtcgagcagc gtcgccggat cgatctggag
     3001 cgcgcgtagc tccgcatctt gcgggccacc gtgaacacgt ggagcgagat gctcaggcgt
     3061 agacatgcgt agctcccagg gcaacgatca cgcagcccgc agcgatgacg gcgcacacca
     3121 tcacgatgcg ccaggcatcg tcgatcttgg cgactgcgag cggctcgacg gcatctccga
     3181 gtcgatagtg cccgaccttc tcgagctgga cccggagcaa tccagccatc gtcgccatcg
     3241 gctggccagc attcgggctc tccgtcgtac gaccatcgcg acggagcatg tgccacccgg
     3301 tcctgacatc tcgccggcac accgcgccag ctgcgagcag cagcacggcc gtgagccgcg
     3361 ccggaatgaa gttgagcgcg tcgtcgagtc gcgccgaggc cttgccgagg tactcgtagc
     3421 ggccgcgata gccgatcatc gcgtcgagcg tattgactgc gcggtaggcc atcgcaccgg
     3481 gcaggccgaa tagcgcgtag tagaacagcg gcgcgacgaa gctgtcggag gtgttctctg
     3541 caaccgactc gaccgtcgcg gctacgagct gcggctcgtc gagcgtgctc ggatctcggc
     3601 tgcacaaact acggagtcct tgccgagccg cgccgagccc gcgtgatgca agcgcgtcgc
     3661 gaaccacgtg cgctgcccgg ccgagcccgc ggatcgcgaa cgtcgatttg agcagcagca
     3721 cgctcgcgat gaatgcagca accggccacg cggcaagctc gcgtagcacg aggaacgtgc
     3781 cgatggcgaa cgcgcaagga accgtaactg cgatgagcgc accggcgacg agctgagcaa
     3841 accggccgcg cgcgggcgac atccgctcga gcagccgcgt gagcttgccc atccatacga
     3901 cgggatggat cgcgttcgga ggctcgccga gcaggacatc gatgccgagc gcggccacga
     3961 gcacccacgc cgacggatcc gagctcgtca tcggttgcgc tccatcgcac gcgctgcagc
     4021 ttgctcgcac ttccactgct ctgccccgct gcgtaccgcg tcgtgaaccg cggcaccaac
     4081 gacgtgaccg agctccgtgt gcttgcccgc gaagcgatgc gggtcggcag tgtcgggcgc
     4141 ggcgatcacg atgcagtcgg tgccggtccc cgaggcaggc aatccgctga tcgggctcgc
     4201 aacctcggcc tcgcgaaccg ccagcgagcg cgcctccgac gccaacgcga gcgcttcgag
     4261 caacgcctcg tcggtcagac cgaccgacac acagcacagc acgttgatcg taccgatgcg
     4321 gccgtctggg cccggcgggt cgccagcgcg caacgcattt ccgagcccga cggtagcgat
     4381 gcaccggcac gacacgtcgc gatacgtccg ctcgagatcg acgtaggggc ggagatcgcg
     4441 actcgtcatc aacgtgacgg cgtcggcaat gccggcagct tcgagccgcg tacgcgcgag
     4501 ctcgcacggg tcgaccggcg gacggagctc gtcgttgcga acttcgaccc acgccacggc
     4561 ccgtgtgcgg cccaatccgc cgccgagcat tgcccaactc accacacgct gtggctcggc
     4621 gaagcgaacc accagcagtc gcggcgcgag ctggaccgtc atcgcggcgg tggtcatcgc
     4681 aggcgctgcc gcgcttcgtt ctcgcagggt cactgcggat tcatctcacg gtacggcgcg
     4741 ctgtgcacac ctcgacgaag ttgcgcgccg ggagcggatc ttctacgcga gcgcgatgtt
     4801 cagggcgttc cacacgccgc gccgccaccg ccggcggctt gtgcgggtgc tgcggcagag
     4861 agctgcttcg cgcaaccggg cagccccgga tttgccttgc agaagctcga tgcgatgccg
     4921 gccgcgtcct gcgcgttgaa cgtctcgcgg ttgttgacga ggaacgtcgg gctcgcctgg
     4981 atgtcgagcg cgctcgcgac cttgaagctc gcggcgagca gacccttgcc ctcctcgcca
     5041 tcgacgcact tcgcgatcac gtcggcgtcg atgccattgc tgcccgtgca cgccttccaa
     5101 tccgccttga ggtccttgct acggcaggcc atgaagtcga ggaacttttc gtccttgccg
     5161 tagtgctgga ccgcgcagat ctcgcggaga tcatcggtaa cctcttcggg cccgtgcatc
     5221 gagctcagct gtccgccctt gtcatcgccg atgaagtgaa cgttgacagc ggcgtccttg
     5281 ccaaaggcgt ccgagaactc cttcgcggcg agcatcgcct tcgttccgta cgggcaatgc
     5341 gacatcacga acagatcgag tgacttcttg atctcggggc gacacgccat cgcgtccttg
     5401 caagcagcat cgtcgcagtc actctgcttg ttgccgtcgt cgtcgacctt gttgtcgcag
     5461 atctcggcgg tcggatcgaa gttgccgccg agcgcgagcg cttggtacgc gccgacggga
     5521 tgcagatagc gcgcgacctg ctgcttgccg tccttgtcgc cgtcgagcga gctatcgaac
     5581 agcacggcag gcagcgtctt gaacgacgcg tcggcttgct tgagctgcgc gtacagcgcc
     5641 ttgccctcgg gcgtcatgta atcgaccttc ttgaccttga ggccctcgaa caaccccgaa
     5701 agctgacctt caagcccgtc gatgttgcac ttcgcgcagc gcgcgtcgga gaagaacacc
     5761 gcgttgacgg ccaccggcgt cgggatcgtc tggcactccg ccggcttctt gctgtcgaac
     5821 gagttgcaga tcgcgcgcag aaaatcgttg gtcttgcgac caccgctgta cggcttgccg
     5881 ttcaggaaca tggtcggcga gccggtggcg ccgcgtttct cggcctcgtc gaacgatgcg
     5941 gcgaggagct gcttgccctt ggtgtcgatg caggacttca ccgcactcgt gtcgatacct
     6001 gcgcgcttgc cgcaggaatc ccagttggtg tcgacgtgcg acggatcctc gttctgacac
     6061 gcgatcatgt tcatgtactt gtcgggcgcg acgtcgcgag cacacagctg cgcgatatcg
     6121 cctgcgacct cgctcggccc gtgcatcgag ctcagctcgt caccctgctt ctggccgatg
     6181 aacgtgagat ggaagtcgac gttgccgccg agcttggcga gaacgggtgc aaccgctttc
     6241 tcgacctgca cgccgtacgg gcacttcgac atcacgaaga actcgagctt cgcgggttga
     6301 tccacggtcc gagccacagg cgccgtggcc atcccggcca tgtgatcgag cttgctgtcg
     6361 ttctcgcacc cgaacagggc ggtggaggcg acgaaggtag tgacgaggat cgtagggatg
     6421 gtggggcgca taaggggatt gaaagacggg aaccgatgca taccagcaaa acattccccg
     6481 ccagcgacgc taagggacat tagtaggccg ctgcccgcgc taacgccggc gagacgacga
     6541 ggagcttgcc ggcgcggcct gcgctgcaag gcgctgccca cgccacgcgt ccattttttt
     6601 ttctgagcgc cgggagcgct gagtgcactc caggaacgcg tgacgattca atgacgtata
     6661 catcttgtat accaacgtcg cagcgcggta caaggctccc caagtacgtc accacaagag
     6721 tagcgcttga cgtgagaggt atatactatg tataccaagt gagcgaaccg gcgttggggg
     6781 gataccgacg ccacaaccag gccggccgac gccggcgctc acatcgcgac aattgctagg
     6841 gggaagacat gcgtactgca gcggtcgagt cacctatctg ggcgaagcgc aactcggcgc
     6901 ctctctcctc actctgcgtc ggttccatgc tcgcagcgct gctcgccggt ggatgcgcga
     6961 caagcgttgc ccctgacgac ccggtgcaag attggaacga cgcgatgaac ctcttcggcg
     7021 agggagcgca aacggtcacg ccgtctgact acggtgcggg tgccacacca cagaacccgc
     7081 cgccaccgtt ccacaccacc gcaaacgtca cgtgcaagcc gatcgtcaac ggaacgagct
     7141 cggtcgtcgt gaacggcagg acccgcaagt tcgaggtcgg cctcccggcg aacctgtcgt
     7201 cgaaggcggc ggtcctcttc gtgtggcacg gcttcctgca ggactcggcc aacttcacga
     7261 acacggtcgt ctacgacccg caggccggcg cgtggaagcc gttcgacacc aacgcgttca
     7321 acatcccgct catcacgatc acgccggtgg actcgaacct catcccggtc tggggactcg
     7381 attgggacat cgtgagcggc gagaaggact tcccgtactt cgaggcgatg ctcacctgcg
     7441 tggaggcgca gttccaggtc gacacgaccc gcgtctactc gttcggcttc tcggccggcg
     7501 ccgtcttcac caatctgctc gcagctgcgt acccgcacct gttcgcagcg acgatcagcg
     7561 aatcgggcac ctggttcaac gacaaggccg agtggtcgga gatcaccgtt ccgctcatcg
     7621 gcacgacgtt catgaagtgg aagtggcccg cgctcactgc tgccgatggt ggtgccgtcc
     7681 tgatgacgca tggcgggccg aacgactttg cgaccgtcat cagtctggag agctcgaacg
     7741 agaaggcgct caacttcctc tacgcgggcg gacgtgacgt cacggagtgc acgcacctgt
     7801 tcggccatac gctcgagccg gacctcacgc aggcgatgta ctactcgttc atgtggcagc
     7861 accagctagg cacgcgtccg ctgacgctcg ctgccgggtt gccgacggcg cagaacccgg
     7921 tcttcgacac ccgctgcaac ttccgcccgg tggtgacgcc cgcagcaccg ggcatcaaca
     7981 acgtcgtgaa cgcgggcttc gagagcgcgc tttccaactg ggcgtacacc ggaggcgttg
     8041 gcttcgacaa cacgacgttc gcgcgcacgg gcagcggcaa cggatgggtc cgcgccaacg
     8101 gggggtggaa tgacgtccac cagatcttca gtgttgttcc gaatcggacg tacacggtgt
     8161 ctggttggat tcggacctcg gacaacaaca ccgacggtta cttcggggtt cgctcggtcg
     8221 ctggagacgt cgtcggcgag cagaagttcg gacgcttcgc cggctacacg aaggtgagtg
     8281 tcaccgttag ctccggctcg aacaccaacc tcgtcgtcta cggcgggctt tgggcgaatg
     8341 gcgatacgtt catgcagctc gacgacgtca gcgtcgtcca gcagtaatcg ttcgtccaag
     8401 gcacgagatc agtggccgcg aaagcggctg ctttcggttt cgggtctcac tctctcaccg
     8461 gcgttgccgg caaggcgttg aggtcgcgac agcgcttcac gctcttgtgt ccgctgtacg
     8521 cggagtacgc caagacggct acgacgccga acagcgcgag ctcggccacc acgaacgttg
     8581 tcgtcgacgt gtcgtcggac ttggttgcga tcgaaccgag accgatgagc gtgagcgcac
     8641 ccgcaccgac cgcatctgcg cccggtggga ccatcgattt ctcacagtcg agcggacgtg
     8701 tccccggatc tttggctggc gcgcgcgtca tgaagaacga gcagccgctg atcagagatc
     8761 cgacgagtat cagtgcgatc agatgacgca tcgattgcaa cgatacgcta cgcgcgccac
     8821 aaccacgcgg gatcattggc gcccgaaaac gtttggacag ttgtcgatct caccgggcct
     8881 cgttcgtcgt ctgctcaaag gagacaccat gaagtacctg tcattcatcc gtcattcaga
     8941 gtcgtatcgc gaggcgacgc cgccacctgc cctcatggag gcgatgggca agttcatcga
     9001 gaagtgccgc gccgacggta cgcttgtcga caccggtggc ctcttgccga gcaaagaagg
     9061 cgtccggatc cggagcagcc gcggcaagct cacggtgaag gatggcccgt tcaccgagtc
     9121 caaagagatc atcggtggct gggcgatcct gcaaggctcc agggaggacg tactccgtgt
     9181 cgccaccgag ttcatggagc tccacgtaag gtactggccc gaattggagt gcgagtccga
     9241 ggtgcgtccg atgttcgacc ctggcgtcgg accctagctt cgacgcctcg catcagtgat
     9301 gtacgcgggc tcccagtctt tgggtctcga gtcccgcgtc ccgagaacac ccgctccatg
     9361 agatccgatg cgttagcgcg gattggagct ggcctgtcgc ttgcgaccag gtgcggggaa
     9421 ggtgtccgcg ccctcgatcg agccccgacc gaagcgcttt ggccgtctcg tatggccggt
     9481 tggcggcgcc acgctcggtg cgctgatcgt ggccctggtt actgcgcgtg ccatgtggag
     9541 cgctcccgag gtggatgtcc aggcaccgat tgtggccgtc gacgaggtcg ctaccgatcc
     9601 cgcggtcgaa gacacctgga cgctgggggc cgagtcgatc gagcggatcg gctatcgatt
     9661 tggccaccgc cacgttgtcg aagtcgttcg actcggtcca ttggccatcg aggtcgagat
     9721 ccgcacggcg cgagcgttcc tcgcgatgcg tgctgccgcc gccgatgcag gtgtggagct
     9781 cggactcgca agcgggtttc ggaccgccga agaacaacgc gagctgtatc gcgcgtggcg
     9841 caaaggtgtc ggcaacaagg ccgcacgacc gggccgctcg aatcatcaga gcggacgtgc
     9901 gctggacatc gccggcgtcg gggctcccgg cgcgctggca tggctcgaag cgaacgctgc
     9961 atcgtttgga ttcacgcgaa cggtgaagaa cgagccgtgg cattgggaat acgtcgacat
    10021 tccgatcgct cgtccggtgg ttgcgcgaat caaacgcacg aaggtcggcc atccgatgcg
    10081 accggcaaag cgctcgaatg cgatcgctac gactcggcag tcgtcgagca aacgcgtggc
    10141 ggcgtcgtcg cagccggatg gtcacgcatc gaagcggaac cggcggatgc cgacgaacat
    10201 gaacagcgct gcgaagccgt agacggcagc gatcggtttg atcacgtcgg caaagcccgt
    10261 cccgtggcgt acgagcagcg cgttatagcc gtcgagcgcc caaccgtgcg gcacgaacaa
    10321 gccgatctgc tgcatgaggt gaggcatcag cacgcgcggc agcatgcagc cgccgacgag
    10381 gcccatgatc aggatcgcga ccgagccaag cgacgagatc tgtttctccg tcgagctcac
    10441 cgacgcgagc agcaggccga ggccggtcgc gctcgctgcg acaccgaccg tgaggaccgc
    10501 gagtgccgcg atgctgccgt tgatctgcat gccgaacccg aacacaccga tcgcgaacag
    10561 caggagcacc tgcagcgtgc cgagtgcgac gtacggcacg agcttcgcgg tcagaagctt
    10621 ccagcgcgag accggcgccg cgagcagacg acgccaggtg cctgtgcggc gttcctcggc
    10681 gaagctgagc gcgcaggtca gcgcgataaa gaagccgaac agcaccgcgt tgccgggcac
    10741 ggtcatctga aacgagtcgg gcgtctcgtg cggctgctgg cgtggggaca cgatcgcgac
    10801 ggtctcggcc cgcggcccca tgacgaccgc atcgacgatc gcgacgactg cgccttggat
    10861 gggcccgcgc tcggcgagcg ccttgtcggt gtcgatcacg agctcggcct tcgcaccacc
    10921 gtgcgcggga tcgaaatcgc gcggcaggat cagtccggca tcgaacgctt cggctgcgac
    10981 ctttgcgcgc acctcctcgg cgctcgcgag cgcgaccggc cggaacatcg tcgagtgcga
    11041 tagcgcagca acgaccgtcg caccgagctg cgaatccggc gccacgtaga ttgcgagtgg
    11101 tcgcccggtg ggcccgtgct cggcatggcg gaacaccatg ccgaacagcg cgatgaacgc
    11161 cacgggcagc gcgagcagtc gaacgagcgc gcgcggatcg cggcgcagca gctgcagctc
    11221 cttcttcacg gtggcaagga acatcacgat gcatctcgca gaccgcgccc ggtgagcgcg
    11281 agaaagacgg tttcgagatc ggcgcgccca gatcccactc gcttgatgcg cgcgcccgtc
    11341 gcttcgatcg ccgtgacgat cggcgccagc gcatccagtt gtgcgaagcg gacgacgtca
    11401 ccgtcgcggg aaccgccgag ttccgcgacc gccttcgccg ccgcgtcgag cgccgtcgga
    11461 tcaccgtcga gctcgagctc gatgcccttc gcaccatgcg cggccgcgag ctctgcaagc
    11521 gatccgctcg cgacgatcgc acccgcgtcc atgatcgcga tgcggtcgca cagctcttcg
    11581 acctcttcca tgtagtggct cgtatagacg actgtcatgc cctggctgcg cagcgcgcga
    11641 atcgagtcga agatgtggcg gcggctctgc ggatcgacac caaccgtcgg ctcgtcgagc
    11701 acgaccaacg tcggcttgtg caa
//
LOCUS       DASUFX010000617        19619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1071741, whole genome shotgun sequence.
ACCESSION   DASUFX010000617 DASUFX010000000
VERSION     DASUFX010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19619
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071741"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VFV99_24800"
     CDS             <1..1302
                     /locus_tag="VFV99_24800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062624.1"
                     /translation="YVVLYDGAGTLTYSGGGSLDAASSTPGRDVVVADGHSPVIVNIT
                     ATTAGNYVRNIRFLMPGGVCSTDAFRYCDDASPCDTGSCSTFETNYATQIFHPTFLSR
                     VRGYRTLRFMDWMATNNSSVSAQGPKVTDARWRGHGVPVEILLELANRIGADAWLNVP
                     HLATDAYVTSLATAVHDRLAPGLKAYIEYSNEVWNGIFSQGTYCEQQGVALGLGSSPF
                     EARLRFQAQRSVEIFEIFETIFPGAERQRLVRVLASQAANSWVSTTLLDHGAALQHTD
                     ALAIAPYFGGYLGGSDHLAAVSAMDLDDLFAELGTVAVPTSLSWVTMQAAVASQRNVS
                     LIAYEAGNHLAGIQGVENNTAVNALFDAANRDPRMRQLYTSYLDGWKANGGELLVHFV
                     NCDAPSKWGRWGALEYLEQPRSEAPKYDAVQTFIETNPAWW"
     gene            1330..1779
                     /locus_tag="VFV99_24805"
     CDS             1330..1779
                     /locus_tag="VFV99_24805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062625.1"
                     /translation="MNCKLCETDCENPEPATTATGVCGSCRNKLGIIPMPPSRRRAKP
                     CEHCNAMRFVRVIPREFTATGGDYVHSSVSPMTATVKPETQAKWVFSGRTVDNPTLSS
                     GAGQLEMYVCTGCGLVEWHCIDPEAIPIGSEYMTEVVDYATGSAYRD"
     gene            complement(1782..3395)
                     /locus_tag="VFV99_24810"
     CDS             complement(1782..3395)
                     /locus_tag="VFV99_24810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5062626.1"
                     /translation="MGSDRGGGELGADSRFRSIVEASPVPYAINDEQQNVTYVNPAFV
                     ATFGYSREDIPTLQKWWPRAYPDPEYRNWVATEWQSRLERARANGAPFEAFEVRIRAK
                     DGTERFAQVGAVSLDGAFRGVHVVVLLDITERKRAEETRLGLERQVQHSQKLESLGVL
                     AGGIAHDFNNLLMAILGNADVALAHLPADSAARMSIQAIQASARRASDVANQMLAYAG
                     KGKLLLAPLSLNDLVSEMSQLLEVAISKKVTLGCNFEPGLPRFEGDATQVRQVIMNLI
                     TNASEAIGDDAGLVTLATGTMQCDRAYLEGPMIVRDQLQNAALREGTYVFLSVADSGC
                     GMTDVTLRRIFEPFFTTKFTGRGLGLSALLGIVRGHAGAIEICSEVGRGTTVRVLFPS
                     TSVVDAAPITAVRTPKAEVVGRPMILFADDEESIRNAVRLMLEHIGFDVVLAANGREA
                     IDVFREREREIAAVILDLTMPVLDGERAFRELRAIRPDVKVILSSGYTSLEVSRRFAT
                     GGLAGFLKKPFDTDELESMLTNTLQIRRS"
     gene            3537..3779
                     /locus_tag="VFV99_24815"
     CDS             3537..3779
                     /locus_tag="VFV99_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062627.1"
                     /translation="MGSITRTIKGKLKKAEGRMTGDKVREAQGAVEEKAGSFGTKVKG
                     NVAKAKAKIAEAKTKAKIKAAKTKAGRKAGAAKAMP"
     gene            3861..4832
                     /locus_tag="VFV99_24820"
     CDS             3861..4832
                     /locus_tag="VFV99_24820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062628.1"
                     /translation="MEPSSTTDGELATGTDVGGYVVQSRLGAGGMGIVYGAVHAGTGK
                     RAAIKVLAPTFCRDPATVERFEQEARLVNDVHHPNIVEVYSLGELADGRKYLAMEWLE
                     GESLSDRIDRGRIPTPEAIEILDGVCDALIRVHAQDVVHRDIKSDNVFLTPAGGSVRP
                     KLLDFGFAKLAAGKNPRQITKTKTGMVVGTPAYLSPEQARGKAADQRSDVYALGILAH
                     KMLTGRLPFEGKVPVDFIVHHLKTQPPDPRELAPDVPEALGLTVVRMMAKTPESRPSL
                     AEIRGVLASLRSPASSPAAPSQFRFVVAIGIVVVVVALAIAIISSVS"
     gene            complement(4833..6104)
                     /locus_tag="VFV99_24825"
     CDS             complement(4833..6104)
                     /locus_tag="VFV99_24825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5062629.1"
                     /translation="MGDTAKPLNAQSRRIRNIYLVLSQFVWLPTSLIVGVNTLFLLDG
                     GLSNVEAFTANGIYTLGLVLFEIPTGMVADTYGRRTSYLLGAAIQLVGNVLYVWMWYV
                     HGPFWGWAIASLLLGFGYTFFSGALEAWLVDALEHAGYDGDLDPIFAKNQIVIGAAML
                     VGTIAGGFIAQAAALGVPYLVRAGLQLVSFVVAFVLMKDIGFSPERAVGFVKQVRALA
                     RAGWTYGIKNRPVRWVMLAAPFYMGTGIYGFYAAQPYLLELFGDKQAIGVAGIAAAGV
                     GATQVAGGFAVPLVRKHFRRRTHMLIACAGLTTVSLVLMGLVQIFAVVVTLLVLWAIA
                     FAVNTPVRQAYINSLIPSNERATVLSFDSMVNSTGGVAFQPILGRVADTAGYAASYIV
                     SGAIAFCGLPFLLLARRAHAHADEIKNDDQA"
     gene            6206..7276
                     /gene="dctP"
                     /locus_tag="VFV99_24830"
     CDS             6206..7276
                     /gene="dctP"
                     /locus_tag="VFV99_24830"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5062630.1"
                     /translation="MKLIAAVGVVLALSSQPVAADSHQFRVATLAPSGSPWMDILDKA
                     SAEITEQTHQRVTIKYYTGGQQGDEQDYIRKIKLGQLDGAAITSIGLAQIEPSILVLQ
                     LPMLFKTQEEVDYVSDKMWPYFQKKFEKKGFRLADRGELGWIYFFSKNKVTSVEDLRK
                     QKLFTIGGDNLSGTLLDKLKLNAVPLGVPEVDAALTSGKINACLSSPLGAIALQWYSK
                     VKYMNSTPMVFGIGATVMSLDTEKKVSAEDRKTIEAIAKRSQKKARAIIRKANEDARK
                     TLVRKGITIVDVPQGMVDELTKVALEVQTELIGKVYSKEELDMVLKYRDEFRAKHAAP
                     AAKTDAAAKPEPAAKTVPAATK"
     gene            complement(7287..7823)
                     /locus_tag="VFV99_24835"
     CDS             complement(7287..7823)
                     /locus_tag="VFV99_24835"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX5062631.1"
                     /translation="MSLASAGERAGVKMADTVNVGGETLTLNGMGLREATVLKVDVYV
                     AGLYLEHVSSNPQQILASNETKVIVLKFKRDVGRDDIVKAWREGFEKNAPVPKPLVDQ
                     LAQWMPSFRKGDVLMFTLMPGKGVAVDINGKRKGVLGDDNFSRSLLSIWLGPNPPTGS
                     LKTGMLGKHAQPQRAQRT"
     gene            complement(7934..9385)
                     /locus_tag="VFV99_24840"
     CDS             complement(7934..9385)
                     /locus_tag="VFV99_24840"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEX5062632.1"
                     /translation="MFGSARAAVFFALFIAACGPTAVHNNQGDDDNTGDASVPACTPG
                     TTQACYTGAPGTMGVGPCVGGMQTCDSGGNWGACDGEVTPKGETCGNGTDENCNGMVD
                     EDTDADGDGFSTCHGDCCDSTADGCSDPQLVNPGAFEAPGNMVDDDCDGTVDNTVAAA
                     CDSGLASNSANALDYAKAIELCQTATMTDTKWGVINARFVLPNGSGTPNADQRSIRPA
                     FGATTVRAGSSFAVLSTGNAAAPGQTNPPYQAFQGGRSVGSSSALPADWLAANGNNVP
                     NAPGCPGATGTTGNDPIMLELTIRTPTNAKSFKLSTNFMSSEYPEWTCSAYNDFFVVL
                     LDSTWNGQPANPADKNLAIYKAPNNSTYPVGVNLAAGNTGLFQQCVNGNTGCALGATA
                     GTINTCVGTNELVGTGMDTANPGGLFGYCGANNLMGGGTGWLVTAGNVKGGEIITLRI
                     AIWDTSDGALDSLAIIDNFEWSVDASQPGTVIF"
     gene            9547..10179
                     /locus_tag="VFV99_24845"
     CDS             9547..10179
                     /locus_tag="VFV99_24845"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX5062633.1"
                     /translation="MRILVLSIALLGCGLSGGETGSGTPKTETREVGAFTKIALEGAL
                     RADITAGAPQLVELSGDDNIVPLITTEVTDQRLRVAPKKRVSPKLELVARIATPTLSA
                     VSSSGSGAVNVTGIAGDSFDLRTSGSAKIVARGTTKKLTIDVSGAATIDAKELAAQDV
                     TINVSGSGELDVRASGHLEVQISGSGTVRYYGNPTDVKKSITGSGTVEAR"
     gene            complement(10182..10382)
                     /locus_tag="VFV99_24850"
     CDS             complement(10182..10382)
                     /locus_tag="VFV99_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HEX5062634.1"
                     /translation="MSHADKRKQSLYFPEDMLKEIQHEAGRLDRSLSWIVQQSWKIAR
                     SKLKDFPSSTDEPGALPDPREE"
     gene            10568..12067
                     /locus_tag="VFV99_24855"
     CDS             10568..12067
                     /locus_tag="VFV99_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5062635.1"
                     /translation="MSTNTPEPQIGRVAARGAVWMIATSIGARVIGLLGTLIVTRFLA
                     PDVMGDIAAAMIVSLTTGCLVTWGFGQYAVVEGRGADALEVTWHATVAYTVVGLVGFG
                     GVTALAPVLADALGAPGAARYIPGFALAAIIRRVGATPERVLTRSLRFRSVAVASAAG
                     EVAYGIVATSLVASGWGGQGVIVGNIVQSSVASALLIRAAGWREWATPVRLRWQRFAR
                     MLRFGVPLAVQSTAHAASRYWGTLLITRLFGSAATGLYSLAYNFADIPAIYIGEQLAL
                     VLMPSMANLPPERRAPALERSTRLLALILFPLAIGLGLVAEPLIAIVLPAKWQGVAPL
                     LVVLSALSVFRPITWTLSAYLEARAQTGRLMLLEVASLIVMLCGIWLLSPLGIVWSAA
                     AVGMAFGLYAIAGVLVVSRSGLSIARLAIGFVQPLVACGAMALVVLMLRPILQATVRL
                     PAQLAVEILVGAFVYVNAALVVCRATARDLIGVVREMIWSRTGRYERRE"
     gene            complement(12049..13116)
                     /locus_tag="VFV99_24860"
     CDS             complement(12049..13116)
                     /locus_tag="VFV99_24860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5062636.1"
                     /translation="MKRVVHVVSSLEIGGAERLVVDLACAQQRAGLGAWIVDLGRDES
                     PLADAARRLGVQVIRAGRMRSRLARTVWLARVLVARENAIHIHNPWALRAVLPILPAI
                     RGRVIYTRHGASPYGGRGWRAVHRLARPFIDQITFVTDEARAAFEAAHWRSVFHHIVI
                     ENGVAVPAAVPDRERPGRLRIGSVGRLVELKGQRLVLDAIAALPPDHRARVEVHLFGD
                     GPDRSSLTDHARASLHDVPVVFHGTVLDRERIYNAIDVLAVGSRTEGQSLAIMEAMAR
                     GVPAIATNVGGNPQFVHDGETGMLVPSGDATAIRDAIARMLVDPDLVSRLGRASHSLI
                     GAYHSIDAVAARYHSLYSRRS"
     gene            complement(13113..14969)
                     /locus_tag="VFV99_24865"
     CDS             complement(13113..14969)
                     /locus_tag="VFV99_24865"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX5062637.1"
                     /translation="MIGSWASTREADANISAMLRALGDRHAATWVSLSSTCRLGVVDR
                     CGTTERPRVLEHGDLVAVCDGRLFDRGDAASADVRLLELWASGGPRAVGRADAQYALA
                     VWDRRAERLTLARDALGVRSLYYHQGRGGVVFASTIEALLCHPAVPRDVDERSVSYFL
                     TFLNVPVPRTLFAGIAKLAPGSFVECSSRGIERTQRCWDLFDDPLPEIDDPRHYALRA
                     SEHHRDAVAARVVDGPMGALLSGGNDSSANVAVMAKLGVSPLHTFTVGLAELEGNERF
                     SDLVHARRVAKIIGTTHHERLISTNEFIASMPRVIDAQDDLVSEPSSVFLHHALDMVR
                     EQGLRVVITGEANDEISSGHGQMLRHRDRYYRRWRPLMLLPRALRQLLAYTVPLISRR
                     HADVLARAAADGEYFWSYELAWTDHAKAEILTPAALARTRDALAAEIVNDRARAIRRA
                     GTQRDYLAHVIGMMMQDHYLGNLMLGKLEQLSSRLGIEARCPYTSPAYVHFVYNIPTR
                     FKARPGQVKTFFKAAIADLLPHDIIYRPKQGFRTPTPELFRGRFGDWAKPLLLETGLT
                     RAGVLRRDTLAAMFAEHRRGSRDLSTRLWTALVLNLWHERWAAPQRARAVNA"
     gene            complement(14984..16150)
                     /locus_tag="VFV99_24870"
     CDS             complement(14984..16150)
                     /locus_tag="VFV99_24870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5062638.1"
                     /translation="MTDAVVYVVDSLGLSGKTRALTNLALNLDRARFRGAVVTFTPPE
                     GMLVEQLRAAGIPVEHVPCEDGLRPAVVGQLVQLFRKIRPAIIHCYNPRPMLYGGLAA
                     AAIARPAIASLSAFACMSDDREYAFLPQPLQTRSRRNRLRNRLLGRLMRRVATVSARL
                     GQRFLEASGLPSRKLRVIGYGVDIDGVDRISEDDVGRVRAELCAAPGEVLVGSVGRLV
                     EQKDYPTQLRALALAARRWPLRMIIAGAGPLASQLRGIAERLGIADRVSWLGERSDIP
                     TMLRCLDAFVIASKFEPFGVSVLEAMAAGLPVVSTDVNELPEILDGGRAGMLVPAERP
                     EDLAVALEQVSADPELRVRLGTHARNIARTRYSLSAAVSAYVNLYEEVINEGVR"
     gene            complement(16147..18063)
                     /gene="asnB"
                     /locus_tag="VFV99_24875"
     CDS             complement(16147..18063)
                     /gene="asnB"
                     /locus_tag="VFV99_24875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5062639.1"
                     /translation="MCGITGVFAFAATARDEEAHLRRMRDTMVHRGPDDAGIWQDRER
                     GIALAHRRLSIVDLSAAGHQPITNEDGSVWLTFNGEIYNHERLRGPLESAGHVFRSRC
                     DAEVILHGYETHGIDVVHHLDGMFAFGLWDAHRRRLLLARDRLGKKPLYYIETGGRLL
                     FASEIKALLAHPDVSRDLDPVALDQYLTFSNVPAPRTLFAGIRKLPPGHVLTSSATEG
                     VAVRRYWSALDQAALGKDVPEQQAVEQVLELLRASVRKRMMSDVPLGALLSGGIDSST
                     NVALMTGLIAQPLRTFSVGFRGFGPDENFHDLPYARAVAARFGCDHQELSVTADECRD
                     HIPELATQLDEPLGDPACLPMHFIARAVRNAGITVVLVGEGSDEVFGGYDAMVHHLDV
                     SIPRFERVRRLPRVLRHGLYHAARLLRLPPGRVDVLRRAAHDEPLYWGLDVAFWEGEK
                     AALLDRARHGSAPGLVRAFYSELAARNPNADHLQQVSWVELCNRLPELLLMRVDRLTM
                     AHSLEARAPFLDPALVSYVLALPSHLKIRGKITKYVLRQAVRSLLPASVLSRPKQGFR
                     VPLPEWLRGDLAVWAEHQLRSAAIHRRGLFRRDAIDRMWERHRSGAYDHSFDLWCLIQ
                     LAAWYERWIEGSAG"
     gene            18289..>19619
                     /locus_tag="VFV99_24880"
     CDS             18289..>19619
                     /locus_tag="VFV99_24880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5062640.1"
                     /translation="MLRTAIAAVVCALAGCVETPEMMPPDSSGTDPTCSNGIKDGLET
                     DVDCGGGTCRRCAGLRHCEAASDCFSGLCAADTNTCPALTTVAFANAVFYPSSYKPYV
                     MLAGDLDADGDVDLAAGNELASTVTVFRNDGSGVFSSLAASTPDGFPTGEYPTGGAIA
                     DLNRDGIADIVTADYHGNSVSVLLGAGSQNTYTLTAGASYPTVPGAETSNLAVGDLNG
                     DSITDVIATNPQMHSVSVFLGRGDGTLQPAVDLGLMGMAEPYSVAIGDFDGDGNNDAA
                     IADNVTLTVYIELGNGDGTFTPAPARPQVDGQASFILLARDMDLDGKLDVVVANRNSE
                     DVSVLLGHGDGTFADAVVSPAGPGGPYSLAVTDFNLDAVPDVVTANFMTGNASVLLGI
                     GNGHFEAPIDAGGTGDFSYGVATGDFNSDGKPDFATANALSNDVAVKMSTA"
BASE COUNT         3475 a         6373 c         6437 g         3334 t
ORIGIN      
        1 tacgtcgtgc tctacgacgg cgccggcacg ctgacgtaca gcggcggtgg ctcactcgat
       61 gctgcgagct cgacgccggg ccgcgatgtc gtcgtcgccg acggccacag ccccgtcatc
      121 gtgaacatca cggcgaccac ggcaggcaat tacgttcgca acatccgctt cctcatgccg
      181 ggcggcgtgt gctcgacgga tgcgttccgc tactgcgacg acgcatcgcc gtgtgacacc
      241 ggcagctgct cgacattcga gaccaactac gcgacgcaga tcttccatcc gacgttcctg
      301 tcgcgggtgc gcggctaccg cacgctgcgg ttcatggact ggatggcgac gaacaactcg
      361 tcggtgtcgg cacagggacc gaaggtgacc gatgcgcgct ggcgcgggca tggcgtgccg
      421 gtcgagatcc tgctcgagct cgcgaaccgg atcggcgctg acgcgtggct caacgttccg
      481 catctcgcaa ccgatgcgta cgtgacgtcg ctcgcgaccg ccgtgcacga tcgcctcgcg
      541 cccggcctca aggcgtacat cgaatactcg aatgaggtct ggaacggcat tttctcgcag
      601 ggcacctact gcgagcagca aggcgtcgcc cttgggctcg gcagctcgcc gttcgaagcg
      661 cggctgcgat tccaggcgca acgctcggtc gagatcttcg agatcttcga gaccatcttc
      721 ccaggcgccg aacggcagcg gctcgtgcgc gtgctcgcat cgcaagcggc gaactcgtgg
      781 gtgtcgacca cgctgctcga ccatggcgca gcgctccagc acaccgacgc actcgcgatc
      841 gcgccgtact tcggtggtta cctcggcggc agcgatcacc tcgccgcggt gagcgcgatg
      901 gatctcgatg acctgttcgc ggagctcggc accgttgcag tgcctacctc gctgagctgg
      961 gtgacgatgc aagcggcggt cgcgagtcag cgcaacgtgt cactgatcgc gtacgaggcc
     1021 ggcaatcacc tcgccggaat ccaaggcgtc gagaacaaca ccgccgtcaa tgcgctgttc
     1081 gatgcggcga accgcgatcc gcgcatgcgc cagctgtaca cgagctatct cgacggttgg
     1141 aaagcgaacg gcggcgagct gctcgtccac ttcgtcaact gcgacgcgcc gagcaagtgg
     1201 ggccgttggg gcgcgctcga atatctcgag caaccacgca gcgaggcgcc gaagtacgac
     1261 gcggtccaga cgttcatcga gacgaacccc gcctggtggt gaggcagaac gcgttacagt
     1321 tcacacgtca tgaactgcaa gctctgcgag accgactgcg agaatccaga acctgcaacg
     1381 accgcgaccg gcgtctgcgg ctcgtgtcgc aacaagctcg gcatcattcc gatgccgccg
     1441 tcgcgccgtc gcgcgaaacc gtgcgagcac tgcaacgcga tgcgattcgt gcgggtgatt
     1501 ccgcgtgagt tcaccgcgac cggcggcgat tacgtgcact cgagcgtttc gccgatgacg
     1561 gcgaccgtga agccggagac gcaggcgaag tgggtgttca gcggtcgcac ggtcgataat
     1621 ccgacgttga gctccggcgc tggacagctc gagatgtacg tctgtaccgg ctgcggcctt
     1681 gtcgaatggc actgcatcga tccggaagcg attccgatcg gcagcgaata catgaccgag
     1741 gtcgtcgact acgcgacagg ctcggcctac cgcgactgag tctagcttcg gcgaatctgg
     1801 agcgtgttcg tgagcatgga ctcgagctcg tcggtgtcga atggcttctt gagaaagcct
     1861 gcgagcccgc cggtcgcgaa gcggcgcgag acctcgaggc tggtgtagcc gctcgagagg
     1921 atgaccttca cgtctggacg aatcgcgcgg agctcgcgaa aggctcgctc gccatccagc
     1981 acgggcatcg tcagatcgag gatgacggcc gcgatttcgc gctcgcgctc gcggaagaca
     2041 tcaatcgcct cgcgcccgtt cgcagcaagc acgacgtcga agccgatgtg ctccaacatc
     2101 aaccgcaccg cgttgcggat cgattcttcg tcgtcggcga acaggatcat cggccgaccg
     2161 acgacttcag ctttcggggt tcgcactgcg gtaatcggcg cggcatcgac cacgctggtc
     2221 gacgggaaca gcacgcgcac cgtggtgccg cggccgacct cgctgcagat ctcgatcgcg
     2281 cccgcgtggc cacgcacgat tccgagcagt gcggacaggc cgagtccgcg acccgtgaac
     2341 ttcgtcgtga agaacggctc gaagatacgc cggagcgtta cgtccgtcat gccgcagccg
     2401 ctgtcggcaa ccgagagaaa cacgtacgtg ccctcgcgca acgcggcatt ttgcaactgg
     2461 tcgcgcacga tcattggccc ctcgaggtac gcgcgatcgc actgcatcgt gccggtggct
     2521 agcgttacga ggccagcatc gtctccgatc gcctcggatg cattcgtgat caagttcatg
     2581 atgacctggc gcacctgcgt ggcgtcgcct tcgaagcgcg ggagacccgg ctcgaagttg
     2641 caacccagcg tgaccttctt cgagatcgcg acttcgagca gctgcgacat ctccgagacc
     2701 aggtcgttga gcgacagcgg cgcgaggagc aacttgcctt tgcccgcata cgcgagcatc
     2761 tggttcgcga cgtcggacgc gcgccgcgcg gacgcctgga tcgcctggat gctcatgcgt
     2821 gcggcgctgt ctgccggtag atgggcgagc gcgacatcgg cgttgccgag aatcgccatg
     2881 agcaggttgt tgaagtcgtg cgcgatgccg ccggcgagca cgccgagact ctcgagcttc
     2941 tgtgaatgtt ggacttggcg ctcgagaccc agccgcgttt cctctgcgcg cttgcgctcg
     3001 gtgatgtcga ggagcacgac gacgtgcacg ccccggaagg cgccgtccag cgatactgca
     3061 ccgacctgcg cgaatcgctc ggtgccatcc ttcgctcgga tcctgacctc gaacgcctcg
     3121 aacggcgcgc catttgcgcg cgcgcgctcg agtcgcgact gccattcggt ggcgacccag
     3181 tttcgatact cggggtcggg atacgcgcgc ggccaccact tctgtagcgt tggaatgtct
     3241 tcgcgcgagt agccgaacgt cgcgacgaac gcggggttca cgtacgtcac gttctgctgc
     3301 tcgtcattga tcgcgtatgg caccggcgat gcctcgacga tgcttcgaaa gcgcgaatcg
     3361 gcccccagct cacctccgcc cctatcgctc cccatttcgg cgcaacgcta gcacctccgg
     3421 cttgcgaagg gctcgtgcgt acgcgcacga ctgagcgcgt gctctacgaa gacggccgtg
     3481 tttgcgaacg cgtcgggcgg catgtggtgt gcaccgtgcg gcggaaggag aatcacatgg
     3541 gaagcatcac tcgtacgatc aaaggaaagc tgaagaaggc cgaaggccgc atgacgggcg
     3601 acaaggtccg cgaggcgcaa ggcgcggtcg aggagaaggc cgggagcttc ggtaccaagg
     3661 tcaagggcaa cgtcgccaag gcgaaagcca agattgccga ggcgaagacg aaggccaaga
     3721 tcaaggcggc gaagacgaag gcgggccgca aggcgggcgc tgctaaagca atgccgtaga
     3781 cgttgcgaca cgcgttgcat tcgcgcgtgc cgtgatcgag tcacgcacgt cgaagcctga
     3841 tacgctggtt aaccgtcgac gtggaacctt cgagcacaac cgatggcgag ctcgcaacgg
     3901 gcaccgatgt aggcggctac gtcgttcaaa gccggcttgg tgcgggcggc atgggtatcg
     3961 tctacggcgc cgtgcatgcg ggcactggca aacgcgcggc gatcaaagtg ctcgcgccga
     4021 cgttctgtcg cgaccccgcg acggtcgagc gcttcgagca agaggcgcgg ctcgtcaacg
     4081 atgtccatca tcccaacatc gtcgaggtgt actcgctcgg cgagctcgcc gacggccgca
     4141 agtacctcgc gatggagtgg ctcgagggcg agagcctcag cgaccggatc gatcgtggtc
     4201 gcattccgac gcccgaagcg atcgagattc tcgacggcgt gtgtgacgcg ctgatccgcg
     4261 tgcacgcgca agacgtcgtc caccgcgaca tcaaatcgga caacgtgttc ctcacgccgg
     4321 cgggcggatc ggtgcggccg aagttgctcg atttcggctt cgcgaagctc gcggccggca
     4381 agaacccgcg ccagatcacg aagaccaaga cgggcatggt ggtcgggacg ccggcctacc
     4441 tgtcacccga gcaggcgcgc ggcaaggcag ccgatcaacg cagcgacgtg tacgcgctcg
     4501 gcatcctcgc gcacaagatg ctcaccgggc ggttgccgtt cgaaggcaag gtgcccgtcg
     4561 atttcatcgt ccatcacctg aagacgcagc cacccgatcc ccgcgagctc gcgcccgacg
     4621 tgcccgaagc gctcggcctc accgtcgtgc gaatgatggc caagacgccc gagtcgcgcc
     4681 cgagtctcgc cgagattcgt ggcgtgctcg ccagccttcg tagccctgca agctcgccgg
     4741 ctgcgccgtc gcagtttcga ttcgtcgtcg cgatcggaat tgttgtcgtt gttgtggctc
     4801 tcgcgatcgc gatcatcagc agcgtgagct agctacgcct ggtcgtcatt cttgatctcg
     4861 tcggcgtgcg cgtgtgcgcg gcgggcgagc aacaagaacg gcaggccgca aaacgcgatc
     4921 gcgcccgaga caatgtacga cgccgcatag ccagcggtgt cggcgactcg cccgaggatc
     4981 ggctgaaacg cgacgccgcc cgtcgaattc accatcgagt cgaatgacag cacggtcgcg
     5041 cgttcgttcg acggaatgag cgaattgata tacgcctgcc tgaccggcgt attcacggcg
     5101 aacgcgatgg cccacagcac gagcagcgtc acgacgacgg cgaagatctg gacgaggccc
     5161 atcagcacga gcgagacggt tgtcagtccc gcgcatgcga tgagcatgtg cgtgcggcgg
     5221 cggaagtgct tccggaccag cggcaccgcg aatccgccgg cgacctgcgt cgcaccgacg
     5281 cctgctgcgg cgatgccggc aaccccgatc gcctgcttat cgccgaacag ctcgagcaga
     5341 tacggctgcg cggcgtagaa cccgtagata ccggtgccca tgtagaacgg cgcggcgagc
     5401 atcacccagc gcaccggccg gttcttgatg ccgtaggtcc agccggcgcg cgcaagcgct
     5461 cgcacttgct tcacgaaccc gacggcgcgt tcgggtgaga agccgatatc cttcatcagc
     5521 acgaacgcga cgacgaagct cacgagctga agcccggcgc gcaccagata cggcacaccg
     5581 agcgcggcgg cctgcgcgat gaagccgccg gcgatcgtac cgacgagcat cgcggcaccg
     5641 atgacgatct gattcttcgc gaagatcgga tcgagatcgc cgtcgtagcc cgcgtgctcg
     5701 agcgcgtcga cgagccacgc ctcgagtgcg cccgagaaga acgtgtaacc aaagccgagc
     5761 agcagcgacg cgatcgccca gccccagaac gggccgtgca cgtaccacat ccagacgtag
     5821 agcacgttgc caacgagctg gatcgcagcg ccgagcaggt acgaagtccg ccgcccgtac
     5881 gtatcggcca ccatcccggt cggaatctcg aacaggacga ggccgagtgt gtagatcccg
     5941 ttggccgtga aggcctcgac gttgctcagc ccgccgtcca gcaagaacag cgtgttgacg
     6001 ccgacgatga gcgaggtcgg gagccagacg aactggctga gaaccaagta gatgttccgg
     6061 atccgtcggc tctgcgcgtt cagtggtttc gcggtgtcgc ccacacacaa aggtactgga
     6121 cgcgcgagac ccagcctacc tgtgacaacg ctgtgactgc gtgatgacca ccgaacggag
     6181 cgcgaatcgc gttagcctcg ggcgcatgaa gctcatcgcg gccgtcggtg tggtgctcgc
     6241 gctttcgtca cagccggtgg ctgccgattc acatcagttc cgggtcgcga cgctcgcgcc
     6301 gtcgggaagc ccgtggatgg acatcctcga taaagcgtcc gccgagatca ccgagcagac
     6361 gcatcaacgg gtcacgatca agtactacac cggtggtcag cagggcgacg agcaggacta
     6421 catccgcaag atcaagctcg gtcagctcga cggagcagcg atcacctcga tcggcctcgc
     6481 gcagatcgag ccatcgatcc tcgtgctcca gttgccgatg ctgttcaaga cgcaagagga
     6541 ggtcgactac gtctccgaca agatgtggcc ctacttccag aagaagttcg aaaagaaggg
     6601 ctttcgtctc gccgaccgcg gcgagctcgg ctggatctat ttcttctcga agaacaaggt
     6661 cacctccgtc gaggatctcc ggaagcaaaa gctcttcacg atcggcggcg acaacctcag
     6721 cggcacgctg ctcgacaagc tgaagctcaa cgccgttccg ctcggcgttc ccgaagtcga
     6781 tgcggcgctg acgtcgggca agatcaacgc gtgcctcagc tcaccactcg gcgcgatcgc
     6841 cctgcagtgg tactcgaagg tcaagtacat gaattcgacg ccgatggtgt tcgggatcgg
     6901 cgccaccgtg atgtctctcg acaccgagaa gaaagtgtcg gccgaagaca gaaagacgat
     6961 cgaagcgatc gcgaagcgaa gccaaaagaa ggcacgcgcg atcattcgca aggcaaacga
     7021 agacgcgcgc aagacgctcg tccgcaaggg catcacgatc gtcgacgtgc ctcaaggcat
     7081 ggtcgacgag ctgaccaagg tcgcgctcga ggttcagacc gagctgatcg gcaaggtcta
     7141 ttcgaaggag gagctcgaca tggtcctcaa gtaccgcgac gagttccgcg ccaagcacgc
     7201 ggcgcccgcc gcgaagaccg acgccgccgc gaagccagaa cctgcggcaa agacagtgcc
     7261 cgcggcgacg aagtagctcg ccggcgctac gtccgttgtg cgcgttgtgg ctgcgcgtgt
     7321 ttgccgagca tgccggtctt caggctgccg gtcggcgggt tgggcccaag ccagatcgac
     7381 agcaacgagc gcgagaagtt gtcgtcgccg agcacgccct tgcgcttgcc gttgatgtcg
     7441 acggcgacgc cctttcccgg catgagcgtg aacatgagca catcgccttt gcggaagctc
     7501 ggcatccact gcgcgagctg gtcgaccaac ggcttcggaa ccggtgcgtt cttctcgaag
     7561 ccttcgcgcc acgccttgac gatgtcgtcg cgaccgacgt cgcgcttgaa cttgagcacg
     7621 atcaccttcg tctcgttcga cgcgaggatc tgctgcgggt tcgacgacac gtgctcgaga
     7681 tataggccag cgacgtagac gtcgaccttg agcaccgtcg cttcgcgcag gcccatcccg
     7741 ttcagcgtga gggtttctcc gccgacgttc acggtatcgg ccatcttcac gccggcgcgt
     7801 tcaccggcgg atgccagcga cactacggcc agcagcgtag tcagaaccat tgcaactcgc
     7861 gccatcgtga cctccacaga ctcgacacga agcaagatgc tcgcccggtc gagtcgggag
     7921 aatcggcaga cagctagaag atcaccgtgc caggctgcga cgcgtcgacg gaccactcga
     7981 agttgtcgat gatcgcgaga gagtcgagcg cgccgtcgct ggtatcccag atcgcgatgc
     8041 gcagtgtgat gatctcgccg cccttgacgt tgccggcggt gacgagccaa ccggtgccgc
     8101 cgcccatcag gttgttcgcg ccgcagtagc cgaacaggcc accggggttc gccgtgtcca
     8161 tgccggtgcc gacaagctcg ttggtgccga cgcacgtgtt gatcgtgccg gcagtcgcac
     8221 cgagcgcgca gcccgtgttt ccgttcacgc actgctggaa caggcccgtg ttgccggccg
     8281 cgaggttcac gcccacggga taggtcgagt tgttcggcgc cttgtagatc gcgaggttct
     8341 tgtcggcggg attggctggc tgaccgttcc atgtcgagtc gagcagcacg acgaagaagt
     8401 cgttgtacgc gctgcatgtc cactcgggat actccgagct catgaagttc gtcgacagct
     8461 tgaacgactt cgcgttcgtc ggcgttcgaa tcgtgagctc gagcatgatc ggatcgttgc
     8521 cggtcgtgcc cgttgccccg gggcagccgg gcgcgttcgg cacgttgttg ccattcgcgg
     8581 ccagccaatc ggcaggaagc gcgctcgacg atccgacgct gcgtccgccc tggaacgctt
     8641 gatacggcgg attcgtctgg ccgggcgccg cagcattacc cgtcgagagc acggcgaacg
     8701 agctgccggc gcgcaccgtt gtcgctccga acgccggacg gatcgagcgc tggtcagcgt
     8761 ttggtgttcc ggagccattc ggcaacacga agcgcgcgtt gatcacgccc cacttcgtat
     8821 cggtcatcgt cgcggtctga cagagctcga tcgcttttgc gtagtcgagc gcgttagccg
     8881 aattgctcgc gagtcccgag tcgcatgctg cggcgacggt gttgtcgacg gtcccatcgc
     8941 aatcgtcgtc gaccatgttg cccggcgcct caaacgcacc cgggttcacg agctgcggat
     9001 cgctacagcc atctgccgtc gaatcgcagc aatcgccgtg gcacgtgctg aagccatcgc
     9061 cgtccgcatc ggtgtcttcg tcgaccatgc cgttgcagtt ctcgtcggtg ccgttgccgc
     9121 acgtttcgcc tttcggcgtc acttcaccgt cgcatgcgcc ccagtttccg ccggagtcac
     9181 acgtctgcat gccgccgacg cacgggccga cgcccatcgt gcccggcgcg ccggtgtaac
     9241 aggcttgcgt cgtgcctggt gtgcacgcgg gcaccgatgc gtcgccggtg ttgtcgtcgt
     9301 cgccttggtt gttgtggact gccgtcggtc cacatgcagc gatgaacagc gcgaagaaaa
     9361 ctgctgcgcg agccgatcca aacatggcgg cctccctctt cgcggcgaag cgtcgatcgc
     9421 ggcgatcctc aacgcaacat gaaacatgcg tcagtacccg gcttctgcag gggactcctg
     9481 atcccacaaa caggggagcg gtggttagcc cacacgtgat cacatcgtcg atgctaaaag
     9541 gtcggcatgc gaattctcgt gttgtcgatc gcgctactcg gttgcggttt gtccgggggc
     9601 gaaacaggca gcggcacgcc gaagacggag acgcgcgagg tcggcgcgtt cacgaagatc
     9661 gcgctcgagg gcgcgctgcg tgcagacatc accgccggcg ctccgcaatt ggtcgagctc
     9721 agcggcgacg acaatattgt tccgctcatc acgaccgagg tcaccgatca gcggctgcgc
     9781 gttgcaccga agaagcgcgt gagcccgaag ctcgagcttg tcgcgcgcat tgccacgccg
     9841 acgttatcgg cggtgtcgtc gtcgggctcg ggagccgtca acgtgacagg catcgcaggt
     9901 gacagcttcg acctgcgcac cagtggctcg gcgaagatcg tcgcgcgcgg gacgacgaag
     9961 aagctcacga tcgacgtatc gggcgcagca acgatcgacg ccaaagagct cgccgcccaa
    10021 gacgtgacga tcaacgtgtc aggcagcggc gagctcgacg tgcgtgcgag cggccacctc
    10081 gaagttcaga tctcgggctc ggggacggtt cgctactatg gcaatccgac cgacgtgaag
    10141 aagtcgatca ctggctccgg aaccgtcgag gcgcgctaac gctattcctc gcgcgggtca
    10201 ggcagcgcgc ccggttcgtc ggtgctcgac ggaaagtctt tgagcttgct gcgggcgatc
    10261 ttccaggact gctggacgat ccaggagagt gagcgatcga ggcgacccgc ttcgtgctgg
    10321 atctccttga gcatgtcttc ggggaagtag agggactgct ttctcttgtc agcgtgggac
    10381 attcggacct cgagggagag aatagtccga ccttcggtcc caagtcccgt tacgtaccgg
    10441 cattggcttc gggggtgcat ctggggcgtg cctggctgtc atcgcgccgg tggttcgggc
    10501 tgctccacgt ttcagtatgt ggcggctcga ctcgctgtga ccgggatgcc tgtcggcgaa
    10561 cggactgatg agcactaaca ctccggaacc ccagattggc cgggtcgctg cccgcggcgc
    10621 cgtgtggatg atcgcgacga gcatcggggc acgtgtcatc gggctgctcg gcacgttgat
    10681 cgtgacgagg tttcttgcgc cagacgtgat gggcgacatc gcggccgcga tgatcgtctc
    10741 gctcacgacc ggctgcctcg tgacgtgggg cttcggtcag tatgcggtcg tcgagggccg
    10801 tggcgctgat gcactcgagg tcacgtggca cgcgaccgtg gcatatacgg tggtcggcct
    10861 cgtcgggttt gggggcgtca cggcactcgc tccggtgctg gcggatgcgc tcggtgcacc
    10921 aggtgcggca cggtacatcc ccggtttcgc tcttgcggcg atcatccgac gcgtcggagc
    10981 gacgcctgaa cgcgtgctca ctcgcagcct ccggttccgc tcggtcgctg tcgcgtcggc
    11041 ggcgggcgag gtcgcgtatg gcatcgtcgc aacatcactg gtcgcgagtg gctggggcgg
    11101 gcagggcgtg atcgtcggca acatcgtcca atcgtcggtt gcgagcgcgc tcttgatccg
    11161 cgccgcggga tggcgcgagt gggcgacgcc ggtgcggctg cgctggcaac ggtttgcacg
    11221 gatgctgcgg tttggtgtgc cgctcgcggt gcagagcact gcgcatgccg caagtcgcta
    11281 ctggggcacg ctcctgatca cgcggctgtt cggttcggca gcgacgggcc tgtacagcct
    11341 cgcctacaac ttcgccgaca tccctgcgat ctacatcggt gagcagctcg cgctcgtgct
    11401 gatgccgtcg atggcgaacc tgccacccga acggcgcgcg cccgcgctcg aacgctcgac
    11461 acggctgctc gcgctgattc tgtttccgct cgcgatcggg ctcggtctcg tcgcagagcc
    11521 gctgatcgcg atcgtgctgc cggcgaagtg gcagggcgtc gctccgctgc tcgtggtgct
    11581 gtcggcgcta tcggtgtttc ggccgatcac gtggacgctg tcggcgtatc tggaggcgcg
    11641 cgcccagacc gggcggttga tgctgctcga ggttgcgagc ctgatcgtca tgctctgcgg
    11701 gatctggctg ttgtcgccgc tcggcatcgt gtggtcggcg gccgcggtcg ggatggcgtt
    11761 cgggctgtac gcgatcgcag gcgtgctggt cgtgtcgcgc agtgggctgt cgatcgcgcg
    11821 actcgcgatc ggcttcgtgc agccgctcgt ggcatgcgga gcgatggcgc ttgtcgtatt
    11881 gatgttgcgg ccgatcctcc aggctacggt acggctgccc gcccagctcg ccgtcgagat
    11941 cctcgtcgga gcgttcgtct acgtgaacgc cgcgctggtg gtgtgccgcg cgaccgcacg
    12001 cgacctgatc ggggtcgtgc gcgagatgat ctggtcgcga accgggcgtt acgagcggcg
    12061 cgagtagagc gaatggtagc gggccgcgac ggcgtcgatc gagtgatagg cgccgatcag
    12121 cgagtggctc gcgcgcccga gccggctcac gaggtcagga tcgacgagca tccgcgcgat
    12181 cgcgtcgcgg atcgccgtcg cgtcgccgga cggaacgagc atgccggtct cgccgtcgtg
    12241 aacgaactgc gggttgccgc cgacgttcgt cgcgatcgcc ggcacgccgc gcgccatcgc
    12301 ctccatgatc gccagcgact ggccttcggt gcgcgatccg accgcgagca cgtcgatcgc
    12361 gttgtagatg cgctcgcgat cgagcacggt gccatggaag acgaccggga cgtcgtgcag
    12421 cgacgcccgc gcgtggtcgg taaggctgct gcgatcggga ccatcgccaa acaggtgaac
    12481 ctcgacgcgc gcgcggtgat cgggcggaag cgcggcaatc gcatcgagca cgaggcgctg
    12541 gcccttcagc tcgacgagcc tgccgaccga gccgatgcgc agccggccag gcctctcacg
    12601 gtcgggcacc gctgcaggga ccgcgacgcc gttctcgatc acgatgtggt gaaacaccga
    12661 gcgccaatgc gcagcctcga acgcggcacg cgcttcgtcg gtgacgaacg tgatttgatc
    12721 gatgaacggg cgcgcgagtc gatgcacggc gcgccagccc cggccgccgt aggggctcgc
    12781 accgtggcgc gtgtagatca cgcgaccgcg aatcgcaggc aagatcggca gcacggcgcg
    12841 gagtgcccac ggattgtgga tgtggatcgc gttctcgcgc gcgactagca cgcgggcgag
    12901 ccataccgtg cgcgccaacc gcgagcgcat gcggccggcc cggatcactt gcaccccgag
    12961 gcggcgtgct gcatccgcga gcgggctttc gtcacgcccg aggtcgacaa tccatgcgcc
    13021 gagcccggcg cgctgttgtg cgcacgcgag atcgacgacg aggcgctcgg caccgccgat
    13081 ctcgaggctg ctgacgacat gcacgacccg cttcatgcgt tcaccgcacg agcacgctgc
    13141 ggggccgccc accgctcgtg ccacaggttc aagacgagcg ctgtccacag tcgggtactg
    13201 aggtcgcgcg aaccgcggcg atgctcggcg aacatggcgg cgagcgtgtc gcgccggagc
    13261 acaccggcgc gtgtcagtcc cgtctcgagc aggagcggct tggcccagtc gccgaaccgt
    13321 ccgcggaaca gctcgggcgt tggcgtccgg aagccctgtt tcggccgata gatgatgtcg
    13381 tgcggtagta ggtccgcgat cgccgctttg aagaacgtct tgacttgacc cgggcgcgcc
    13441 ttgaagcgcg tcgggatatt gtagacgaag tgcacgtacg cgggcgacgt atacgggcac
    13501 cgcgcttcga tgccgagccg cgacgacagc tgctcgagct tgccgagcat cagatttccc
    13561 aggtagtggt cctgcatcat catgccgatc acatgcgcga ggtagtcgcg ctgggtcccg
    13621 gcgcggcgga tcgcgcgcgc gcgatcgttg acgatttccg ccgccagcgc gtcacgcgtt
    13681 cgggcgagcg ctgcgggtgt caggatctcg gccttcgcgt gatcggtcca cgcgagctcg
    13741 tagctccaga aatattctcc gtcggcggcg gcgcgtgcga gcacatcggc atggcgccgt
    13801 gaaatcaacg gcaccgtgta cgcgagtagc tggcgcaacg cgcggggtag cagcatcaac
    13861 ggccgccatc ggcggtaata gcggtcgcgg tgacggagca tctggccgtg gcccgacgag
    13921 atctcgtcgt tcgcttcgcc ggtgatcacg acgcgcagtc cctgctcgcg caccatgtcg
    13981 agcgcgtggt ggagaaacac gctcgacggc tccgagacca gatcgtcctg cgcatcgatc
    14041 acgcgcggca tgctggcgat gaattcattc gtcgagatca ggcgctcgtg atgcgtcgtg
    14101 ccgatgatct tcgccacacg gcgtgcgtgg acgagatcgc tgaagcgctc gtttccctcg
    14161 agctccgcga gaccgacggt gaacgtgtgc agcggcgaga cgccgagctt cgccatcact
    14221 gcgacgttcg cgctcgaatc gttacctccg ctgagcagcg cccccatcgg gccgtcgacg
    14281 acgcgagccg caaccgcatc gcgatggtgt tcgctcgcgc ggagcgcgta gtgcctcgga
    14341 tcgtcgatct ccggcagcgg atcgtcgaac agatcccagc atcgctgcgt gcgttcgatg
    14401 ccgcgcgagc tgcattcgac gaacgaaccg ggtgccagct tggcgatgcc ggcaaacagc
    14461 gtgcgcggta ccggtacgtt caagaacgtg aggaagtacg acaccgagcg ctcgtcgacg
    14521 tcgcgcggga ctgcgggatg acagagcagc gcctcgatcg tcgatgcgaa cacgaccccg
    14581 ccgcggccct ggtgatagta gagcgaccgt acgccgagcg cgtcgcgcgc gagcgtcagc
    14641 cgttcggcgc ggcggtccca cacggcaagc gcgtattgcg cgtcggcacg gccgactgcg
    14701 cgcggcccac cactcgccca tagctcgagc aatcgcacgt cagcgctggc cgcgtcaccg
    14761 cgatcgaaca ggcgtccgtc gcataccgcg acgagatcgc cgtgctcgag cacgcgcggt
    14821 cgttctgtcg tgccgcaccg atcgacgacg ccgagccggc aggtcgacga caaactgacc
    14881 cacgttgccg cgtggcggtc gccgagtgcg cggagcatcg cgctgatgtt tgcgtcggcc
    14941 tcgcgggtcg acgcccaact tccgatcatt ccgcagatcg ccgtcatcgc actccctcgt
    15001 tgatcacttc ctcgtagaga ttcacgtacg ccgacaccgc agcgctcagg ctgtagcgag
    15061 tacgcgcgat gttgcgcgca tgcgttccga gtcggacacg cagctccgga tcggcagaga
    15121 cttgttcgag cgcgaccgcg agatcttcgg gacgctcggc gggaaccagc atgccggcac
    15181 gcccaccgtc gaggatctcg ggcagctcgt tgacgtcagt cgacacgacc ggcagtccag
    15241 ccgccatcgc ttcgagcacg ctcacgccga acggctcgaa cttcgatgcg atcacaaacg
    15301 cgtcgaggca gcgcagcatc gtcgggatgt cgctgcgctc gccgagccag ctcacacggt
    15361 cggcgatccc cagtcgctcc gcgatcccac gcagctggct cgcaagcgga cccgcaccgg
    15421 cgatgatcat acgcaacggc cagcgccgag ccgcgagcgc gagcgcacgc agctgcgttg
    15481 gatagtcctt ctgctcgacg aggcgtccga cgctgccgac cagtacctcg ccgggcgctg
    15541 cgcaaagctc ggcgcgcaca cggccgacgt cgtcctcgga gatgcgatcg acaccgtcga
    15601 tgtcgacgcc gtagccgatc acgcgcagct tccgcgacgg cagtccgctc gcttccagga
    15661 accgctgacc gagccgcgcg gacaccgtgg ccacgcgcct catcaggcgt ccgagtagcc
    15721 ggttgcgcag ccgattgcgt cgcgatcgcg tctgcaatgg ctgcggcaag aacgcgtact
    15781 cgcggtcgtc gctcatgcac gcgaacgcgc tgagcgaagc gatcgccggg cgcgcgatcg
    15841 cagcggcggc gagcccgccg tagagcatcg gtcgcgggtt gtaacaatga atgatcgcgg
    15901 gccggatctt gcgaaacagc tgcacgagct ggccgacgac agcaggtcgc agtccgtcct
    15961 cgcacggcac atgctcgacc gggataccgg cggcacgaag ctgctcgacg agcatccctt
    16021 cgggcggcgt gaacgtcacg acggcacctc ggaagcgggc gcgatcgagg ttcaacgcga
    16081 gattcgtgag cgctcgcgtc ttacccgaga gccccagtga atcgacgaca tacacaacag
    16141 cgtccgtcat cctgcactcc cttcgatcca gcgctcgtac caagcggcga gttggatcag
    16201 gcaccacaga tcaaagctgt ggtcgtacgc cccagaacga tgtcgctccc acatgcgatc
    16261 gatcgcgtca cgtcggaaca gcccgcgtcg atggatcgca gcactgcgaa gctgatgctc
    16321 cgcccacaca gcgaggtctc cgcgcaacca ctcgggcaac gggacccgaa acccttgctt
    16381 cggtcgtgag agaacgctcg caggcaacag cgatcgcacc gcctgccgca acacgtactt
    16441 cgtgatcttg ccgcggatct tgagatgcga tggcagcgcg agcacgtacg acacgagcgc
    16501 ggggtcgagg aatggcgcgc gcgcttcgag tgagtgcgcc atcgtcaacc ggtccacgcg
    16561 catgagcagc agctcgggca agcgattaca cagctcgacc cacgacacct gctgcaggtg
    16621 atccgcgttt gggttgcgtg ccgcaagctc gctgtagaac gcgcgcacga gcccgggtgc
    16681 gctcccatgc cgcgctcgat cgagcagcgc tgccttctcg ccttcccaga acgcgacgtc
    16741 gaggccccag tagagcggct cgtcgtgtgc ggcgcggcgt aacacgtcga cgcgacccgg
    16801 cggcaggcgt agcaaccgcg ctgcgtgata gagcccgtgc cgcagcacgc gtggaagccg
    16861 tcgcacgcgc tcgaagcgcg ggatcgatac gtcgagatga tggaccatcg cgtcgtagcc
    16921 gccgaacacc tcgtcgcttc cctcgccaac gagcacgacg gtgatccccg cgttgcgcac
    16981 tgcacgcgcg atgaagtgca tcggcaggca cgcgggatcg ccgagcggct cgtcgagttg
    17041 cgtcgccagc tcggggatgt gatcgcgaca ctcgtctgcg gtgaccgaga gctcttgatg
    17101 atcgcaaccg aagcgcgcag cgaccgcccg tgcgtacggc aggtcgtgaa agttttcgtc
    17161 cggaccaaat ccgcgaaagc cgaccgagaa cgttcgcagc ggctgcgcga tcaggccggt
    17221 catcagtgcg acgttggtgc tggaatcgat cccgccgctc aagagcgcgc cgagcgggac
    17281 atcgctcatc attcgtttcc gcacggacgc gcgcagcaac tcgagcacct gctcgaccgc
    17341 ttgttgctcg ggtacgtctt tgccaagtgc cgcctgatcg agggcggacc agtaccggcg
    17401 aaccgcaaca ccctcggtgg cgctgctcgt gagcacgtga ccaggtggca gcttgcggat
    17461 ccccgcgaac agcgtgcgcg gcgcgggtac gttggaaaac gtcaggtact gatcgagagc
    17521 gaccggatcg agatcgcgcg agacatcggg atgggcgagc agcgccttga tctcggatgc
    17581 gaacagtaag cggccaccgg tctcgatgta gtagagcggc ttcttaccga gtcgatcgcg
    17641 tgcgagtagg agtcggcgcc tgtgcgcgtc ccacaggccg aacgcaaaca tgccgtcgag
    17701 gtgatggacg acgtcgatac cgtgcgtctc gtagccgtgc aggatcactt cggcatcgca
    17761 tcgcgatcga aacacatggc cggcgctttc cagcgggcca cgtagtcgct cgtggttata
    17821 gatctcgccg ttgaacgtca gccataccga tccatcctcg ttcgtgatcg gttgatgacc
    17881 ggccgcagaa agatcgacga tgctcagtcg ccggtgggct agcgcgatac cgcgttcgcg
    17941 atcctgccag atgcccgcat cgtccggtcc gcggtgaacc atcgtgtcgc gcatgcggcg
    18001 cagatgcgct tcttcatcgc gggccgttgc ggcgaacgca aacacgccgg tgatgccgca
    18061 catcgcgccg gacggtgtgc aagaaccagt acctaggtcg cgacgcgcac ttcgctgcca
    18121 cgcgcacggg agctgtcatt cgctgatcat cttcttccgc tcaccgtgca cgcgtgctcg
    18181 ctccgatcgc atcgtgacgc cgaatcgccg tgactcgctt atcgattgtt cgtcacgcga
    18241 cctgcattac ggcgcttctc atcggaggaa acgatgcacg aaagcacgat gcttcgtacg
    18301 gcgatcgccg cagtcgtttg cgcactcgcg ggttgtgtcg agacaccaga gatgatgccg
    18361 ccggattcgt cgggtactga cccgacgtgc tcgaacggga tcaaggacgg gctcgagacc
    18421 gacgtcgact gcggaggcgg cacgtgccgt agatgcgccg gtctcaggca ctgcgaagcg
    18481 gcgagcgatt gtttcagcgg actgtgcgcg gcggacacca acacgtgccc cgcgctgacc
    18541 acggtggcgt tcgcaaacgc cgtgttctat ccgtccagtt acaagccgta cgtcatgctc
    18601 gccggcgatc tcgacgccga cggtgatgtc gacctcgcgg cagggaacga gctcgccagc
    18661 acggtcacgg tatttcgcaa cgatggctcc ggcgtcttct cttcgctcgc cgcgtctact
    18721 ccagatggat tcccgaccgg tgagtacccg accggtggcg ccatcgccga tctcaatcgc
    18781 gacggcatcg ccgacatcgt caccgccgac tatcacggta attcggtgag cgtgttgctc
    18841 ggcgcgggat cgcaaaacac gtacacgctc actgccggcg caagctaccc gaccgtcccg
    18901 ggcgccgaga cctcgaatct cgcggtcggc gatctgaacg gcgattcgat caccgatgtg
    18961 atcgcgacca acccacagat gcattcggtg agcgtcttcc tcgggcgcgg cgatggcacg
    19021 ttgcagcccg cggtcgatct cggactcatg gggatggccg agccgtactc ggtcgcgatc
    19081 ggtgacttcg acggcgatgg caacaatgat gcggcgatcg cagacaacgt cacgttgacg
    19141 gtctacatcg agctcggcaa cggcgatggc acgttcacgc cggcgcccgc acggccacaa
    19201 gtcgatggtc aggcctcgtt catcctcctc gcacgcgaca tggatctcga cggaaagctc
    19261 gacgtcgttg tcgccaaccg caatagcgaa gacgtgagcg tcctgctcgg ccacggtgat
    19321 ggaacgttcg ccgacgcagt cgtctcgccc gcaggcccgg gcgggcctta ttcgctcgcg
    19381 gtgacagact tcaacctcga cgcggttccc gacgtcgtca ccgcgaactt catgaccggt
    19441 aacgcgagcg tgctcctcgg tatcggcaac ggtcacttcg aggcgccgat cgatgccggc
    19501 ggcacgggcg acttcagcta cggcgtcgca accggcgact tcaacagcga cggcaagccc
    19561 gacttcgcta cggcgaacgc gctctccaac gatgtcgcgg tcaagatgag caccgcgca
//
LOCUS       DASUFX010000618         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_933804,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000618 DASUFX010000000
VERSION     DASUFX010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933804"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1819
                     /locus_tag="VFV99_24885"
     CDS             239..1819
                     /locus_tag="VFV99_24885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX5062641.1"
                     /translation="MIASLLETGFLLLAVLVAVEAIANRTHISSSILMVIAGCAMALI
                     PGLPDFTLIPEMVLLLVLPPIIYLAGVQMSWREFHENLRPIALLAIGCVVFTTCAVAA
                     VAHYVLNLSWPVGFVLGAIVAPPDVIAPLAIARRLGIPRRILVILEGEGLANDATALV
                     LYRFAVAAVSIGVFSFRGIIVTFVLILVLEVAYGIALGWASLRLRRWIDDPRLEITLS
                     LMTPYAAFWVPEHLGGSGVIATIATGLYVSWNGPLLISSSTRLQGLFFWDLLIYLIEG
                     LVFLITGLQARTLFERIELDELTRGLVATGLTTAVVIASRFAWIFAGRGRNPSSKHPT
                     ATWQETFLLSFIGVRGVVSLTVALALPLSTASGAPFPARDLILFVTFGLIVITLVGQG
                     LLIPSVIKWLGLGKGAALEHDRQLIEEYRARTLSLDAAGRRLDQLAAERHIAQEAVQF
                     VRAHHDERSQMIPADLDEGLAMTQAGAALRIEAIEEERHEIHRLERIGQLTDEARRRI
                     ERELDLEEEILGSRKPPG"
     gene            1857..3197
                     /locus_tag="VFV99_24890"
     CDS             1857..3197
                     /locus_tag="VFV99_24890"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5062642.1"
                     /translation="MCRAAACRLPAAWSTYGMHTELLVLLILVLANGLFAGAEIAVLT
                     ASKAALEHRKGRRSLAVKQLRDKPERFLATVQIGITVIGAAAAAFGGASIARDLAPGL
                     EPFFGSKAHEVAMVIVVAVVSFLSLVLGELVPKSLALRYSTGYALLIGRPLYGLSRLM
                     RPLVWFLTLCSNAVLKLFGDKTSFIEARMSRDELRELVEDAAKTGSVDMKASEIAARA
                     LEFGDVMVGEVMVRRDRMVAIPGGASAEEVQRILLEEGHSRMPVYDETPDRVIGYIVA
                     RDVLALAWEGNLIALDDIVRPVIYVPSTARISSVLREMQAKRTQLAIVVDEHGLSVGL
                     VTIEDLMEELVGDIYGEHELPEASLHLDADGTALVPGWVPTRKVNRVLHTALPIARES
                     MTLAGLCMALALAVPAVGSKLRAPDGTMLEVTEASPRRVRMVRVYIRGATDENS"
BASE COUNT          511 a         1006 c         1102 g          619 t
ORIGIN      
        1 cgacacgatg gatccggcgt acatggagaa gatgtcgaaa cagatcaagg gcgcgcggtt
       61 cctgctgtgc cccaacggct cgcatctggc gatgtacgac gattcggcga cgttcttccc
      121 cggcctcatc tcgttcatca aggacgtcga cgccggcaag tcgtagtcac agcgctggcc
      181 accgcactac gtagaccgcg cacgtgctcg tagcgcgaga aaggcgctag atcggtggat
      241 gatcgcgtcg ctgctggaga cgggcttcct gctcctcgcg gtgctggtcg ccgtcgaggc
      301 gatcgccaat cgcacgcaca tctcgtcgtc gattctgatg gtcatcgcgg gctgtgcgat
      361 ggcgctcatc cccgggcttc cggacttcac gctcattccg gagatggtgc tcttgctcgt
      421 gctgccgccg atcatttacc tggcgggcgt gcagatgagc tggcgcgagt tccacgagaa
      481 cttgcggccg atcgcactgc tcgccatcgg ttgcgtcgtg ttcacgacgt gcgccgttgc
      541 ggcggtggcg cactacgtat tgaaccttag ctggccggtg ggcttcgtgc tcggtgcgat
      601 cgtcgcaccg cccgacgtga tcgcgccgct cgcgatcgcg cggcggctcg gcattccgcg
      661 ccggatcctc gtcatcctcg aaggggaagg actcgcgaac gatgcgactg cactcgtgct
      721 ctaccgcttt gcggtcgcag cggtttcgat cggcgtgttc tcgttccgag gcatcatcgt
      781 cacgttcgtg ctgatcctcg tgctcgaggt cgcatatggc atcgcgctcg gctgggcgtc
      841 actgcggctg cgacgctgga tcgatgatcc acggctcgaa atcacgctgt cactgatgac
      901 gccgtatgcg gcgttctggg tgccggagca tctcggcggc tcgggtgtga tcgcgacgat
      961 tgcgacgggt ttgtacgtga gctggaacgg cccgctgctg atctcgagct cgacgcgact
     1021 gcagggtctg ttcttttggg atctgttgat ctatttgatc gagggcctcg tattcctcat
     1081 caccgggctg caagcacgca cgttgttcga gcgcatcgag ctcgacgagc tcacgcgcgg
     1141 gctcgtcgcg accggcctca cgacggcggt cgtgatcgcg agtcggttcg cgtggatctt
     1201 cgccgggcga ggccgcaatc cgtcatcgaa acatccgacc gcgacgtggc aggagacgtt
     1261 cctgttgtcg ttcatcggtg tgcgtggggt cgtgtcactc accgtcgcgc tcgcgctgcc
     1321 gctgtcgacc gcctcgggcg cgccattccc cgcgcgcgac ttgatcctgt tcgtcacgtt
     1381 cggtctcatc gtgatcacgc tcgtcggtca gggactgctg attccgtccg tgatcaaatg
     1441 gcttggtctc ggcaagggcg ccgcactcga gcacgatcgc cagctgatcg aggaatatcg
     1501 tgcgcgcacg ctgtcgctcg acgctgccgg gcgcaggctc gatcagctcg cagccgagcg
     1561 gcacatcgcg caagaggcgg tgcagttcgt gcgcgcgcat cacgacgagc gcagccagat
     1621 gatcccggcg gacctcgacg aaggactcgc gatgacgcag gccggcgcgg cgctgcgcat
     1681 cgaggcgatc gaggaggagc ggcacgagat ccaccggctg gagcggatcg gtcagctcac
     1741 ggacgaggcg cggcgccgca tcgaacgcga gctcgatctc gaagaagaaa tcctcgggag
     1801 ccgcaagcca cctggctgat cgaccggctg taacgcccga aaactgtgtc gagaatgtgt
     1861 gcagggcggc ggcgtgccgg ttgcccgccg cgtggtcaac ttacggtatg cacacggagc
     1921 tgctggtcct gctgatcctc gtgctagcga acgggctgtt tgcaggcgcc gagattgcgg
     1981 ttctgacggc ctcgaaggcc gcgctcgagc accggaaagg ccggcgaagc ctcgcggtga
     2041 agcagctgcg cgacaaaccg gagcggtttc tcgcgaccgt gcaaatcggc atcactgtga
     2101 tcggtgctgc ggcagctgcg ttcggtggtg cgagcatcgc gcgcgatctg gcgccgggac
     2161 tcgaaccatt cttcggcagc aaggcacacg aagtcgcgat ggtgatcgtc gtcgcggtgg
     2221 tgtcgttctt gtcgctcgtg ctcggcgagc tggtgccgaa gtcgctcgcg ctgcgctact
     2281 cgaccggcta tgcgctgctg atcggtaggc cactgtacgg attgtcgcgg ctgatgcggc
     2341 cgctggtctg gtttctgacg ttgtgctcga acgccgtcct gaagctgttc ggcgacaaga
     2401 cgtcgttcat cgaggcacgg atgtcgcgcg acgagctacg cgagctcgtc gaggatgcgg
     2461 cgaagacggg ctcggtcgat atgaaggcga gcgagatcgc agcgcgcgcg ctcgaattcg
     2521 gtgacgtcat ggtcggcgag gtgatggttc gtcgcgatcg catggtcgcg atcccgggtg
     2581 gcgcgtccgc cgaggaagtg cagcgcatcc tgctcgagga aggccacagc cgcatgcccg
     2641 tgtatgacga aacgcccgat cgtgtgatcg gctacatcgt cgcgcgtgac gtgctcgcgc
     2701 ttgcatggga gggcaacctg atcgcgctcg atgacatcgt gcgaccggtg atctacgtgc
     2761 cgtcgaccgc gcggatttcg agcgtgctgc gcgagatgca ggccaagcgt acgcagctcg
     2821 cgatcgtcgt cgacgagcac ggcctcagtg tcggtctcgt gacgatcgag gatctcatgg
     2881 aggagctcgt cggcgacatc tacggtgagc acgagctgcc cgaggcatcg ctgcacctcg
     2941 acgccgacgg caccgcgctc gtgcctggtt gggtgccgac gcgcaaagta aatcgcgtgc
     3001 tccacacggc attaccgatc gcgcgcgaga gcatgacgct cgcgggcttg tgcatggcgc
     3061 tcgcgttggc cgtacccgca gtcggcagca agctgcgcgc accggatggc acgatgctcg
     3121 aagtgaccga agcgtcaccg cggcgcgtgc gcatggtgcg cgtgtacatc cgcggcgcga
     3181 ccgacgagaa cagctaaccg ccgctgcaca cgagcgcgtt gccgccattc tcgcggcc
//
LOCUS       DASUFX010000619        10616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_377647,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000619 DASUFX010000000
VERSION     DASUFX010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377647"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VFV99_24895"
     CDS             <1..1941
                     /locus_tag="VFV99_24895"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX5062643.1"
                     /translation="SLGAGLETLRAAVDAAIEAGDVIRQSQWANDLLSKDPTDVRALV
                     ARGSAMVASQPEAARALLELAAARDDVDAHVQLAKLTLATDPVSAAKSALSALRTAPQ
                     HTKAREILAAARAAEYPEPVSSEIADVADYLEKVVASKRGLGHFVGETARAAANLDQP
                     LLVTVMGEFSSGKSSFVNAFIGADVAPTGITPTTATINVVRYGRERGGRIIGRDGATL
                     ELGWEPLMAHLRELTPDASRAIDRVEILVPLPQLEKINIVDTPGLNSIQPEHEATARG
                     FIARADAVVWVFTASQGGKASEKKALQSIRDEGKRVLGVLNKADQLSDSESKEVVEFI
                     GGTLGELVETIVPFSARYALDHKRSGTGNDGNWPALAGALEERFFQQARQLKRDACAR
                     TLRAVVQEAQAVLAKERDRAVEAADTARGARDELMTSAQQFTKEGVVGERKALSDQMA
                     TLYRHAAREVLDLVRPRRLPFSSHTATTADRDYLIALLASGFETAIETGRRRVASELA
                     LRSRKAEAAAKALENALGFDVVGDLERVAADRIGLAMSRVFDRARAYLRGYLEGGYVE
                     AFFRNDVPRLELAEDAIYHALYRSAPDLDREVGDPLAHAATDALSALARRLDHWSSVV
                     DVRMFDMEVGVVRALEIVATRL"
     gene            1973..2686
                     /locus_tag="VFV99_24900"
     CDS             1973..2686
                     /locus_tag="VFV99_24900"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062644.1"
                     /translation="MTKRRNPIPNRMTLGIDEAGRGPAIGPMVMAAVAIDSKTAASLT
                     RKGLRDSKAFGAGDDAHAIRCELAAEIRQRAVFLAVVEIEHDEIDRRVCKGELNALER
                     EIAGKLIEQALGQMPGCDKIIADGKRMFQALTMRYSQFVSVDRAEEHHASVAAASVIA
                     KTIRDDRFNKIKGRYEPELGPISGGGYANAATRKWVRAYVEKYGRLPDEARLSWPYPF
                     VADLLGTVRPLTSQVEMFA"
     gene            2696..3643
                     /locus_tag="VFV99_24905"
     CDS             2696..3643
                     /locus_tag="VFV99_24905"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5062645.1"
                     /translation="MRHAWVVVVLAACGGTEPEPMDTGWTTGADVAQGAIQETAAVAV
                     AGKVYLIGGLDDSEGTVARVQVYDTATNTWSDGPPLPQPVHHANAITDGTTIYVLGAL
                     TNVTAIGDSYKLDPATDTQWTTLAPMSPGRERGAAVVGMLGDNIFVAGGLRNAGASDF
                     LDVYQTVTNTWMPLTSMPAARDHACGAELSGQLIVAGGRMVQTDSPRSEVWAYSVGSN
                     TWEVRASMPTARGGMGCGVVYDRLYTAGGEGNPAVASGVFPNVEEYVYSTDTWTRLAD
                     MPNPKHGVGGAAWNGALYLCGGASMEGVGAIATTDVFRP"
     gene            complement(3683..4660)
                     /locus_tag="VFV99_24910"
     CDS             complement(3683..4660)
                     /locus_tag="VFV99_24910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062646.1"
                     /translation="MTSHDSGGDGDDDAQLKSLRAVWLSMPDEDPPERGLAELMAAAR
                     VKAEQMAKPSLWQRLAALLRRPPVLALATVLVLIGGAVFIGQRHSKMEVAPEASDQRA
                     RMNAESATSSAASGSAAMGPANTLAAPEAPAGAAESAPADMESAQPTPAVEAKPEPKK
                     HEAPKIEAPPRVTHRPPPSSAGVKTATTGAPTTKMNDSVMEKSALSTDSKETKKSDSR
                     GFDGTNGLIEGTLDENQSTDKSSPPPPAAPAEAPTADDRAGGTKGAATAQPQPEVRRA
                     QQYIAQAKTAAARGDCAAARTLMKRVASEDAASYRKAIANDAALKKCFE"
     gene            complement(4657..5259)
                     /locus_tag="VFV99_24915"
     CDS             complement(4657..5259)
                     /locus_tag="VFV99_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5062647.1"
                     /translation="MFNDTADEDLMVMYQKGEVRAFEVLLSRHRKPVYNFILRFVGDK
                     ETAEDLLQEAFMRVIKGAEAYKRQAKFTTWLYTIARNLCVDQTRRRKHRKHASLDAPM
                     DTSEESGTLMDVIPGNEMASDRKSVNKQLHEQMQRAIGALSDEQREVFLMREFLDMPF
                     KQIADVVGVPENTVKSRMRYALEKLRLELDEYKDLAKAAP"
     gene            complement(5324..6151)
                     /gene="mutM"
                     /locus_tag="VFV99_24920"
     CDS             complement(5324..6151)
                     /gene="mutM"
                     /locus_tag="VFV99_24920"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5062648.1"
                     /translation="MPELPEVETVRRTLAPAVGAKIRGVWDSGQGLHMRRKPPRKRLQ
                     HLVGATLTEMRRWGKYLLIDTDTPYTLLIHLGMTGRVLITPASAPRPKHTHLVLDLGD
                     RELRFTDPRRFGQIDIVERAHEREHSGLALLGPDPLVHGIDVAAFTAAAKKKKTTLKA
                     FVLDQSVLAGVGNIYASEALWKAGLRPTKRANKLTPEAAKRLAAAIDEVLHHALHNGG
                     TTLSDFVAADGAEGNNADYLWVYGRAGEKCLRCKKSVIRRAVHQGRATYFCPTCQTP"
     gene            complement(6167..7231)
                     /locus_tag="VFV99_24925"
     CDS             complement(6167..7231)
                     /locus_tag="VFV99_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062649.1"
                     /translation="MRSSIWLLALPFIVGCVAPNSDDEFDDIPDAPFGGKGDSSRIVV
                     SAQSVDELGGDLSSLTPPCRTADPYHDCEFYLSSASALGDFGPLGAYGPLGTLGPLGS
                     NAWNTSYWIGAAGDWSSWAKNMTGLGGPLSEQGPLGPNGPLGDTAYYETLPSINDWAK
                     QLQGGGVWTSLGPLGALGPLGPLGPLGPIGAHGYRVDDHGRYVSGGSEKRTIRVDYAA
                     HTFELVEMYDEDTAKSYAPNDTSFVSLGEVSRGDVDAYDIKSRYDQFVTITVVPEKQL
                     DNFDITIRTPQGRVLATSHSSSYIDWVQLQVPRGGRMTVEVSLAATGHFLSNTYRLYV
                     VGAGEKFSETDIHGDHQQPL"
     gene            complement(7353..8288)
                     /gene="rsmA"
                     /locus_tag="VFV99_24930"
     CDS             complement(7353..8288)
                     /gene="rsmA"
                     /locus_tag="VFV99_24930"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5062650.1"
                     /translation="MSGGGKNGNGSSNGNGNGNGQHALAKEAVGPFPDARVLLDRYGL
                     RAKKSFGQNFLVSDRALRAIADAAVSSDDDWIIEIGAGLGTLTARIAERVTAGKVIAL
                     EPDPDMIRVLRAELGDVDNLEIEQVDALRYDLRAVASLGQKITVCGNLPYHLASQLIF
                     KVIDVRDVVRFAVFMIQKEMADRIVAKPGTKEYGALGVMVRTYCDVQAVIKVGAGSFV
                     PAPKIDSTVIKMVPLPNAEPRVRISDHAHYSKVVHAAFGQRRKTLRNALRAHWSDEEV
                     DAALAASSVDGSRRGETLDIVEFGRLAEALPVAGA"
     gene            complement(8285..9319)
                     /gene="tsaD"
                     /locus_tag="VFV99_24935"
     CDS             complement(8285..9319)
                     /gene="tsaD"
                     /locus_tag="VFV99_24935"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5062651.1"
                     /translation="MTDARPYRVLGIESSCDETAAAVVEDGRIVRSDVVASQNEVHAR
                     YGGVVPELASRAHILNVVPVVQAALDRASVTLDDIDGIAVTNAPGLVGALLVGLQTAK
                     ALAWVTGKPLVGVHHLEGHLSAIFLEPDPPPMPHVALIVSGGHTSLVRVNGHGDLIEL
                     GATRDDAAGEAFDKGAKLLGLGYPGGAVIDKLAQTGDPNAVAFPRSMTQGEHGADFSF
                     SGLKTALLHHVRAHGVPDDQPLADLCASYQAAIVEVLVRKTRRAARRLQLHHVQVCGG
                     VAANSGLRRAMQAAGEEDDFRVYVPPIARCTDNAAMIAAAGYHRLMRGERDGLALDAV
                     PSAPLARSQA"
     gene            complement(9316..>10616)
                     /locus_tag="VFV99_24940"
     CDS             complement(9316..>10616)
                     /locus_tag="VFV99_24940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062652.1"
                     /translation="MDADPYTSGEHPLPSEEELAPFVEADPYTSGEMSAVSAEDYSGV
                     PTVVEADDRSGDILDEGPATDPARRRFANTVRRDDSQGLRMIGSHGRDRAEASGELMP
                     PPSRPQTADGEPQREMVTILPRRITREIAIQPAPDDKTAEAKADAKTEADAEAKPETG
                     KPAVEPPPRPPPRRVTVEGGVVRQAGPGRGAKVLIGIAMVLAGATVYSYCKKPKPTQP
                     QVATFEDAAVIATPTEDAAQLSGFGIDAAVAVVTPVDGAVAVVPHVDAAVAVATPVDA
                     AVVAVRPDAAVRPDAAVVAVVRPDAAVSTSAADAGVDKTKEARALYDKAHEALEDGDA
                     QQALDLLDQSIKLRKTARSFLERARALQRLGKVDDAVAAVDEAIKMNSTYAPAYEQKG
                     MILWSAQRYGDARPALEKYLELEPDGRSAAKIRAMLDEPK"
BASE COUNT         1720 a         3492 c         3547 g         1857 t
ORIGIN      
        1 tcgcttggag cagggctcga gacgctgcgc gccgccgtcg acgccgcgat cgaagcgggc
       61 gacgtgatcc ggcagtcgca gtgggcgaat gatctgttga gcaaggaccc gacggacgtc
      121 cgcgcgctcg tcgcgcgtgg ctcggcgatg gtcgcgtcgc agcccgaggc tgcacgtgcg
      181 ttgctcgagc tggcagccgc ccgcgacgac gtcgatgccc acgtgcaact cgcgaagctg
      241 acgctcgcga cggatccggt aagcgctgcg aagtccgcac tctcggcgct gaggaccgca
      301 ccgcaacaca cgaaggcgcg cgagattctc gctgcggcac gcgccgccga gtaccccgag
      361 ccggtgtcga gcgagatcgc cgatgtcgcc gactatctcg agaaggtggt cgcgtcgaag
      421 cgcgggctcg gtcacttcgt cggcgagacc gcacgcgcgg ctgcgaacct cgatcagccg
      481 ttgctcgtga cggtgatggg cgagttctcg agcggcaagt catcgttcgt caatgcattc
      541 atcggcgccg atgtcgcgcc gaccggtatc acgccgacga ccgcgacgat caacgtcgtg
      601 cgctatggac gcgagcgcgg tggccgcatc atcggccgcg acggcgcgac gctcgagctc
      661 ggctgggaac cgctgatggc gcatctgcgc gagctgacgc ccgacgcatc gcgagcgatc
      721 gatcgcgtcg agatcttggt accgctaccg cagctcgaga agatcaacat cgtcgacacg
      781 ccgggcctca actcgatcca gcccgagcac gaagcgaccg cgcgcggctt catcgcgcgc
      841 gctgacgcgg tcgtctgggt gttcaccgcg tcgcagggtg gcaaggcgag cgagaagaag
      901 gcgctgcagt cgattcgcga cgagggcaag cgcgtgctcg gtgtgctcaa caaggccgac
      961 cagctgagcg acagcgagag caaggaagtc gtcgagttca tcggcggcac gctcggcgag
     1021 ctcgtcgaga ccatcgtgcc gttctcggcg cggtacgcgc tcgatcacaa gcgcagcggg
     1081 acggggaacg acgggaactg gcccgcgctc gccggtgcac tcgaggagcg gttctttcag
     1141 caagcacgcc agctcaagcg cgatgcgtgc gcgcgtacgc tgcgcgcagt cgtgcaggag
     1201 gcccaggcgg tgctcgccaa agagcgggat cgtgcggtcg aagccgcgga tacggcgcgt
     1261 ggcgcacgcg acgagctgat gacctcggca cagcagttca ccaaggaggg cgtcgtcggc
     1321 gaacgcaaag cgctctccga tcagatggcg acgctgtacc ggcatgccgc acgcgaggtc
     1381 ctcgatctcg tgcggccgcg acggctgccg ttctcgtcgc acaccgcgac gacggctgat
     1441 cgcgactatc tgatcgcgtt gctcgcatcg ggattcgaga ccgcgatcga aaccgggcgc
     1501 cggcgtgtcg cgagcgagct ggcgctgcgc agccgcaagg ccgaggcggc ggcaaaggcg
     1561 ctcgagaacg cactcggctt cgatgtcgtc ggtgatctcg aacgcgtggc ggccgaccgc
     1621 atcggcctcg cgatgtcgcg cgtgttcgat cgcgcacgcg cgtacctgcg cggctatctc
     1681 gaaggcggtt atgtcgaggc gttcttccgc aacgatgtgc cccggctcga gctcgcggag
     1741 gacgcgatct atcacgccct gtatcgatcc gctccggatc tcgaccgcga ggtcggggat
     1801 cctctcgcgc acgcggcgac cgacgcgctc tcagcactcg cccggcggct cgatcactgg
     1861 agctcggtcg tcgatgttcg gatgtttgat atggaagttg gcgtggttcg ggctctcgag
     1921 atcgtcgcga ctcgtctatg atgtcgcacg tctcccttag actccgccga tcatgacgaa
     1981 gcgacgcaat ccgatcccga atagaatgac gctcgggatc gatgaggcgg gacgcggccc
     2041 ggcgatcggg ccgatggtga tggcggcggt tgcgatcgac agcaagactg cggcgtcgct
     2101 cacgcgcaaa ggcctgcgcg actccaaggc gttcggcgcc ggagacgacg cgcacgcgat
     2161 ccgctgcgag ctggcagccg agatccggca gcgcgcggtg ttcctcgcag tcgtcgagat
     2221 cgagcacgac gagatcgatc gccgcgtctg caaaggcgag ctcaacgcgc tcgagcgcga
     2281 gattgccggc aagctcatcg agcaggccct cggccagatg ccgggctgcg acaagatcat
     2341 cgcggacggc aagcggatgt tccaggccct gacgatgagg tactcgcagt tcgtttcggt
     2401 cgatcgcgcc gaggagcatc acgcgtcggt ggccgctgcg tcggtgatcg caaagacgat
     2461 ccgcgacgac cgattcaaca agatcaaggg ccgctacgag cccgagctcg gcccgatttc
     2521 gggcggcggc tatgcgaatg ccgcgacgcg caagtgggtg cgtgcgtacg tcgagaagta
     2581 cggccggctg cccgacgaag cgcgcctgtc gtggccgtat ccgttcgtcg ccgatttgct
     2641 cggaacggtg cgtccgctca cgtcgcaagt cgaaatgttc gcgtaaggtc tcctcatgcg
     2701 tcacgcgtgg gtcgttgtcg tgctcgcggc gtgcggtggc accgagccgg agccgatgga
     2761 cacgggctgg acgacggggg ctgacgtcgc gcaaggcgcg atccaggaga ccgcggcggt
     2821 cgcagttgcc ggcaaggtct atctgatcgg cggtctcgac gacagcgaag gcaccgttgc
     2881 gcgcgtgcag gtctacgaca ccgcgaccaa cacatggagc gatggtccgc cgctgccaca
     2941 gcccgtgcat cacgcgaatg cgatcaccga tggcacgacg atctacgtgc tcggcgcgct
     3001 caccaatgtc accgcgatcg gcgacagcta caagctcgat cccgcgaccg acacgcagtg
     3061 gacgacgctc gcgccgatgt cgcccggccg cgaacgcggt gctgccgtcg tcggcatgct
     3121 cggcgacaac atcttcgtcg cgggcgggct tcgcaatgcc ggcgcgagcg atttcctcga
     3181 cgtttaccaa acggtcacga atacgtggat gccgctaaca tcgatgcccg ccgcgcgcga
     3241 tcacgcttgc ggtgccgagc tttctggcca gctgatcgtg gcgggcggac gcatggtcca
     3301 gacggattcg ccgcgctcgg aggtgtgggc gtattccgtg ggctcgaaca cgtgggaggt
     3361 gcgtgcgtcg atgccgactg cgcgaggcgg catgggctgc ggcgtggttt acgaccggct
     3421 ctacaccgcg ggcggcgaag gcaatcctgc ggtcgcgtcg ggcgtgttcc cgaacgtcga
     3481 ggagtacgtc tactcgacgg acacctggac gaggctcgcg gacatgccga acccgaagca
     3541 tggcgttggc ggcgcggcct ggaacggtgc gctctacttg tgcggcggtg cgagcatgga
     3601 aggcgtcggc gcgatcgcga cgaccgacgt gtttcggccg tagtccgagc atccggcagg
     3661 agtcaggtcg agccggacct gattactcga agcacttttt tagcgccgca tcattcgcga
     3721 tcgctttgcg atagctcgcc gcgtcttcgc tcgcgacgcg cttcatcaac gtgcgagcgg
     3781 cggcacaatc accacgcgcc gcagcggtct tcgcctgcgc gatgtactgc tgcgcgcgcc
     3841 tcacctcggg ctgcggttgc gccgtcgcag caccctttgt tccgcctgcg cgatcgtctg
     3901 ccgtcggcgc ttcggccggt gccgccggcg gtggcggaga agacttatcg gtcgactggt
     3961 tctcgtcgag cgtgccctcg atcaggccgt tcgtaccatc gaagccgcgc gagtcggatt
     4021 tcttggtctc tttggagtcg gtcgacagcg cgctcttctc catcaccgag tcgttcatct
     4081 tggtcgttgg agccccggtc gttgcggtct tcacccccgc actgcttggc ggcggccgat
     4141 gcgtcacgcg cggcggcgct tcgatcttgg gcgcctcgtg cttcttcggc tcgggtttcg
     4201 cttcgaccgc cggtgtcggc tgtgcggatt ccatgtcggc cggagccgac tccgccgcgc
     4261 ccgccggagc ttctggagcc gcgagcgtat tggcaggccc catggcggcg gagccgcttg
     4321 ccgcggagct ggtggccgac tcggcgttca tgcgcgctcg ctgatcgctg gcctccggcg
     4381 ccacttccat cttcgagtga cgctggccga tgaacaccgc gccgccgatg agcacgagca
     4441 cggtcgcgag tgcgagcacg ggagggcgac ggagcagcgc ggccagccgc tgccacagcg
     4501 acggcttcgc catctgctcg gccttcacgc gcgcagccgc catcagctcg gcgaggcctc
     4561 gctcgggcgg atcctcgtcg ggcatcgaca gccacaccgc gcggagcgac ttcagctgcg
     4621 catcgtcgtc gccgtcgccg ccagaatcgt gagacgtcac ggcgcggcct tcgccagatc
     4681 cttgtattcg tcgagctcga gtcgcagctt ctcgagcgca tagcgcatgc ggctcttcac
     4741 ggtgttctcg ggcacgccga cgacatccgc gatctgcttg aacggcatgt cgaggaactc
     4801 gcgcatcaag aacacttcac gctgctcgtc ggacagcgca ccgatcgcgc gctgcatctg
     4861 ttcgtgcagc tgcttgttca cactcttgcg atcgctggcc atctcgttgc cggggatcac
     4921 atccatcaac gtgccgctct cttcgctggt gtccatcgga gcatccagcg acgcgtgctt
     4981 gcgatgtttg cgtctcctag tctggtcaac gcacaagttc ctcgcgatgg tgtaaagcca
     5041 cgtcgtgaat ttggcctgcc gcttgtaagc ctccgcgccc ttgatcacgc gcatgaaggc
     5101 ttcttggagc aggtcctccg ccgtttcctt atcgccgacg aaccgaagaa tgaagttgta
     5161 gacgggcttg cgatgacgcg acagcaagac ctcgaaggcg cgaacttcgc ccttctggta
     5221 catgaccatg aggtcttcgt cggcagtatc gttgaacatt cgcgcccagg atcacaacgc
     5281 cggactcggc tcacactaac acgaagccca aagatcagac cggctatggg gtctgacacg
     5341 ttggacagaa ataggtggcc cggccttggt gcacggcccg acgaattacc gacttcttac
     5401 accgcaggca cttctcacca gcacgtccgt agacccacag atagtccgca ttgttgccct
     5461 cggcgccgtc ggccgcgacg aagtcggaca gcgtcgtccc gccgttgtgc agcgcgtggt
     5521 gcagcacttc gtcgatcgcc gctgcgagcc gcttcgccgc ctcgggcgtg agcttgttcg
     5581 cgcgcttcgt cgggcgcagc cccgctttcc atagcgcttc ggacgcgtag atgttgccga
     5641 caccggcgag cacggactgg tcgagcacga acgctttgag cgtggtcttc ttcttcttcg
     5701 cggctgcagt gaacgccgcg acgtcgatgc cgtggacgag tggatcggga ccgagcagtg
     5761 cgaggccgga atgctcgcgt tcgtgcgcgc gctcgacgat atcgatctgg ccgaagcggc
     5821 gtggatcggt gaagcgcaac tcgcgatcac cgaggtcgag cacgaggtgc gtatgcttcg
     5881 ggcgcggcgc gctcgccggc gtgatcagca cgcggccggt catgccgaga tggatcagca
     5941 gcgtgtacgg cgtgtcggtg tcgatcagca ggtacttgcc ccaccgccgc atctcggtga
     6001 gcgtcgcgcc gacgagatgt tgcagccgct tgcgcggcgg cttgcggcgc atgtgcaggc
     6061 cctgaccgct gtcccagacg ccacggatct tcgcgccgac tgcgggcgcg agcgtgcggc
     6121 gaacggtctc gacttcgggc agctcgggca tgcccgctgc ttagcactag agcggctgct
     6181 ggtggtcgcc gtggatgtcg gtctcggaga acttctcgcc agcaccgacc acgtagagtc
     6241 ggtaggtgtt cgacaggaag tggccggtcg cggcgagcga cacctcgaca gtcattcggc
     6301 cgccgcgggg aacttggagc tgcacccagt cgatgtacga ggacgagtgc gacgtcgcga
     6361 gcacgcggcc ctgtggcgtc ctgatcgtga tgtcgaagtt gtcgagctgc ttctccggca
     6421 cgaccgtgat cgtcacgaac tggtcgtagc gcgacttgat gtcgtacgcg tcgacgtcgc
     6481 cgcgcgaaac ttcaccgagc gagacgaacg aggtgtcgtt cggcgcgtag ctcttcgccg
     6541 tgtcttcgtc gtacatctcg acgagctcga acgtgtgcgc cgcgtaatcg acacgaatcg
     6601 tgcgcttctc cgagccgccg ctgacgtacc ggccgtggtc gtcgacgcga taaccgtgcg
     6661 caccgatcgg accgagcggc cccaacgggc cgagcggacc gagtgcaccg agtggaccga
     6721 ggctcgtcca cacgccgccg ccttgcagct gcttcgccca atcattgatc gacggcagcg
     6781 tctcgtaata cgccgtgtcg ccgagcggac cattcggacc gagcgggcct tgctcgctga
     6841 gcggaccacc gaggcctgtc atgttcttcg cccacgacga ccagtcgccc gcggcgccga
     6901 tccaatacga cgtgttccac gcattcgaac cgagtggacc cagcgtgccg agcggaccgt
     6961 acgcgccaag tgggccgaag tcgccgagcg cgcttgccga cgacagatag aattcgcaat
     7021 cgtgatacgg gtccgccgtt cggcacggcg gcgtgagcga ggacaggtca ccgcctagct
     7081 cgtcgacaga ctgcgcgctg acgacgattc tcgacgagtc accttttccg ccaaaaggcg
     7141 cgtccggaat gtcgtcaaat tcgtcgtcgg aatttggagc aacacagcca acaatgaacg
     7201 gcaacgcgag gagccagatt gatgaacgca tgtcctttcg gacagcaacc gtcgatccgt
     7261 gaacacgtcg cggtaaccaa ccgatcagtc agcgcttcgc gttgagctgt atttagactt
     7321 gccggcgacc gttcctagcg tggccggcgt tatcaggcgc cggctactgg aagagcttcc
     7381 gccagtcgac cgaactcgac gatgtcgagc gtctctcctc gacgagaacc atcgacgctg
     7441 gaggcggcaa gtgcagcatc cacttcctcg tcggaccagt gcgcgcgaag cgcattccgc
     7501 agggtttttc gtcgttgacc aaacgctgcg tgcacgacct tcgagtagtg cgcatggtcc
     7561 gagatccgga cacgtggctc tgcgttcggc agcggcacca tcttgatcac cgtcgagtcg
     7621 atcttcggcg cgggcacgaa cgagccggcg ccaactttga tcaccgcctg cacatcgcag
     7681 tacgtgcgca ccatgacgcc gagcgcgccg tactccttcg tgccgggctt tgcgacgatg
     7741 cgatccgcca tctccttctg gatcatgaac acggcgaagc gtacgacgtc gcgcacgtcg
     7801 atcactttga agatgagctg cgacgcgagg tgatagggaa gattgccgca cacggtgatt
     7861 ttctggccga gcgacgcgac ggcgcgcaga tcgtagcgaa gcgcatcgac ctgctcgatc
     7921 tcgaggttgt cgacatcgcc gagctcggcg cgcagcacgc ggatcatgtc cggatcgggc
     7981 tcgagcgcga tcaccttgcc cgcggtcacg cgctcggcga ttcgagcggt cagcgtcccg
     8041 agaccggcgc cgatctcgat gatccaatcg tcatcggacg acaccgctgc atcggcaatc
     8101 gctcgcagcg cacgatcgct gaccagaaag ttctggccga aggatttctt cgcgcgcaag
     8161 ccatagcgat cgagcaggac gcgcgcatcg ggaaacgggc cgacggcttc cttggccagc
     8221 gcgtgctggc cgttgccgtt gccattgccg ttgctgctgc cgttgccgtt cttgccgccg
     8281 ccgctcacgc ctgactccgc gcgagtggcg cgctcggaac cgcatcgagc gccaggccat
     8341 cgcgttcgcc gcgcatcaat cggtggtagc ccgcggcggc gatcatcgcg gcgttatcgg
     8401 tgcagcgtgc gatcggtggc acgtacacgc ggaagtcgtc ctcttcgccg gccgcctgca
     8461 tcgcgcggcg caggcccgag ttcgcagcga cgccgccgca aacctgcacg tgatggagct
     8521 ggagccggcg ggcagcgcgc cgcgtcttgc gcacgagcac ctcgacgatc gcggcctggt
     8581 agctcgcgca gagatctgcg agcggttgat cgtcgggtac gccgtgcgcg cgcacgtgat
     8641 gaagcaacgc ggtcttgagc ccggagaacg aaaagtccgc gccgtgctcg ccttgggtca
     8701 tcgagcgcgg aaacgcgacc gcgtttggat cgccggtctg cgcgagcttg tcgatgactg
     8761 cgccaccggg atacccgagg ccgagcagct tcgcgccctt gtcgaacgct tcgccggccg
     8821 cgtcgtcgcg cgtcgcaccg agctcgatca ggtcgccgtg gccgttcacg cggacgagcg
     8881 acgtatgccc gccggaaacg atcagcgcga cgtgaggcat cggcggcggg tctggctcga
     8941 ggaagatcgc gctgagatga ccttcgaggt gatgcacgcc gacgagcggc ttgccagtca
     9001 cccatgcgag cgccttcgcg gtctgcaagc cgacgagcag cgcaccgacg agccccggcg
     9061 cgttcgtgac cgcgatgcca tcgatgtcat cgagcgtgac gctcgcgcga tcgagcgccg
     9121 cttgcacgac cggcacgaca ttcaggatgt gcgcacgcga tgcaagctcg ggcacgacgc
     9181 cgccgtagcg agcgtggact tcgttttgtg acgcgaccac gtccgaacgc acgatgcggc
     9241 cgtcttcgac gacagctgcg gctgtctcgt cgcacgagct ctcgatgccg agcacgcgat
     9301 acgggcgcgc gtcggtcatt tcggttcgtc gagcatggcg cggatctttg ccgccgaccg
     9361 gccgtctggc tcgagctcga gatacttctc gagcgccggc cgtgcatcac cgtagcgctg
     9421 cgccgaccac aggatcatgc ccttctgttc gtacgctggt gcgtacgtgc tgttcatctt
     9481 gatcgcttca tcgacggcgg cgaccgcatc gtcgacctta ccgaggcgct gcagcgcacg
     9541 cgcacgctcg aggaacgagc gcgcggtctt gcgcagcttg atcgattgat cgagcaggtc
     9601 gagtgcttgc tgggcgtcac catcttcgag cgcctcgtgc gccttgtcgt agagagcgcg
     9661 cgcttccttc gtcttgtcca cgccggcatc ggcggcggac gtcgagaccg ctgcgtcggg
     9721 gcgaacgaca gcgacaaccg cagcatccgg gcgcacggct gcgtcgggac gaaccgcgac
     9781 gaccgctgcg tccaccggtg tagcgaccgc gacagcagca tcgacgtgtg gcacgaccgc
     9841 gaccgcgccg tcgaccggcg taacaactgc gaccgcagca tcgataccaa agccgctcag
     9901 ctgcgctgca tcttcggtcg gcgtcgcgat aaccgcggcg tcctcgaagg tcgcgacttg
     9961 cggctgcgtg ggcttcggct tcttgcagta gctgtagacc gtggcgccgg cgagcaccat
    10021 cgcgatgccg atcagaacct tggcgcccct gcccggccca gcctggcgaa cgacaccgcc
    10081 ttcgacggtc acccggcgtg gcggtggacg cggcggcggc tcgacggccg gtttgccggt
    10141 ctcgggcttc gcctcggcgt cggcttcggt tttcgcgtcg gccttcgcct cggcagtctt
    10201 gtcgtcgggc gccggttgga tcgcgatctc gcgcgtgatc cggcgcggca ggatcgtgac
    10261 catctcgcgc tgcggttcac cgtccgcggt ttgcggacgc gacggcggcg gcatcagctc
    10321 gccagacgct tcggcgcgat cacggccgtg cgagccgatc atgcgcaggc cctgtgagtc
    10381 gtcgcggcgg accgtgttgg cgaatcgacg gcgcgccgga tcggtcgccg gaccttcgtc
    10441 gagaatatcg cctgaccgat cgtctgcttc gacaacggtc ggcacgcccg agtagtcctc
    10501 cgcagacacc gcggacatct cgccactcgt gtacggatcg gcctcgacga atggggccag
    10561 ctcctcttcg cttggaagcg gatgctcgcc gctcgtatac ggatcggcgt ccatca
//
LOCUS       DASUFX010000620        15102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2045075, whole genome shotgun sequence.
ACCESSION   DASUFX010000620 DASUFX010000000
VERSION     DASUFX010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045075"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFV99_24945"
     CDS             complement(<1..1085)
                     /locus_tag="VFV99_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062653.1"
                     /translation="MRRSRARLLAAAVVVSTAGAASASLDLVVHGDPSPYGARAVVVL
                     GAIGGDGGTIDDTPLAGSDGPSDGFDAPMEGGFDAPMEGGLDGPVGSDGGGIPDAPPI
                     TTIDIMEPTIAITGPQGVLSQATATVITMDTGQLGSAAIFSTDSSMKLSYCGLTTCNW
                     SPPEVLPYSLTVECVPGPMQTIATLQVFEASNGSPAMANVTCTSTSSNPVLNVTPDSL
                     DFSDVPVGQTPSQMIYVENTGFGTLNNVVIDFGATTNANQWSASACTAASPCSFGAGS
                     GSFVSITFAPTTHGAKNVLATVTSANGGTDTVSLTGTGTGGVMSVQTPTGNPPVLDIG
                     TIPKGQAFSRDIVLANSGNAMYTATTST"
     gene            complement(1092..3683)
                     /locus_tag="VFV99_24950"
     CDS             complement(1092..3683)
                     /locus_tag="VFV99_24950"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5062654.1"
                     /translation="MLVRGIAVALVSGAALVAYAVGNGDIRANPEVTVITITSATGTG
                     TGTASLQNTTGATTYSVLVGSDASCDPSLMFSVVGGNPVVISPTTMRNVNLTCPARGT
                     PAMRRCLYHATNNTNGTALADFMTVCLYGNAPGTLAPQQTTIDFGTVTVGDNAMQQID
                     VRNNGTLGQFITRVYLQTDDLGGNFQFSNPCNPDAPFCDEDVMAIGQGGTLPIQVKCT
                     PQTPGLHTAQLYIGTNTFQLLAQPVTLTCNGAATTVPVLGLNPTNISLPTPVDAASGS
                     ASTVVHLTNAGAGSLVIHDVRTVDVDAGAANDWTYAASGECSGQITMSCSLEPGEQVD
                     INLTFDPSGIGRRRATLLVSYKDSIDRTKEIPLDGRSIGGTLDVAGDVSTLVFGSVPV
                     GRSSAIDFALTNHGNLDLDVNLALAASSTPPFTLSPAASTVVMANVDRAVTLTCAPTT
                     ASQFMTSVTATAMDAVNGSPTTLNATCEGSTLDLFANPTALNLGEVRAGGGEVRRTIQ
                     LLNTNAPSQLTLNSQMQVEGNAPGLTLGALSQLTTPATFDVVLDPPADADEGQMTGAI
                     IVTDTAGEMLRIPLSGKVVRAGYEAANALDLGTFCVGQPTTSSNVSLVSDGTATIALM
                     QPTLGQVPSPFELETTSPSVYPTVIAAGAAATVSITPHRQTAVVQLDDTLAWHTDVAG
                     METATTAITARFIDQGGAIAPPALDFGKALVHLYSEDGQRVMIQNCNPTPLQLDEPMI
                     KTPFKLESPLPSVLNPNETAAFSVGFHPTQIGVVETVLRITSPQLPGAPLEVMLRGEG
                     TSQDIMEPDAGMGSNGTDDTSFYACSCKSTRPGGILPIVLALMCVLFPRRSPSFSRRR
                     RPRVRIP"
     gene            complement(3698..5044)
                     /locus_tag="VFV99_24955"
     CDS             complement(3698..5044)
                     /locus_tag="VFV99_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062655.1"
                     /translation="MRGRLSIALALSASAAYAQPVTNPDAVPPLAQIRDEKQLAEALA
                     TITQDPAIRVDDEKNRPLAAALMTEGVRQLQAQGYEQALANFLEAYGKFPSPKILLNI
                     GSTLRDMGRLADAANTYQRYLADPATGPERIAEVKELLVKLDQELTVLTLRVFPRGSE
                     VSIDAGPFIPVGSTLQTRVRAGIHLVRVRKGDATTELTINGFEGESKDITTKVEPKAA
                     PSPTPPTNPTNPTPPTNPTAAATPPKPPEPAPDNVDGWLITGRQYATENVSGGERHVL
                     AGYGGPVMRPIVPHYTIEDDGSVTVAPPPSERRITSGVLGVLRVDGKGRGVAGGLGIA
                     LSPLDSVELELAGLKAEDWGVYFGMRIRFLTGWLRPYAGGGVPLWFYTDETTMESRIA
                     LGLRGAGGVELKLNGHVSIQADVGIEHFFNVKDALVHGKRPDETVFVPTVGVIGRL"
     gene            complement(5051..6337)
                     /locus_tag="VFV99_24960"
     CDS             complement(5051..6337)
                     /locus_tag="VFV99_24960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062656.1"
                     /translation="MEEPNLVGTMLGSYRITGELSRGGMGKVYRAEHSVLERTVAIKL
                     LRPELTTNDELVSRFITEAKAASAIRHPGIIEVLDFGHASDGRAYFVMEMLKGESLAR
                     RIESRGRLSPREAAKIARDIAAALTAAHAKGIVHRDLKPDNVFLIPDPDLGERPKVLD
                     FGIAKLSDTNHHTQTGALIGTPLYMAPEQARAASGIDHRADLYSVGCMLYEMLCGQPP
                     FVAVGAGEIIALQMFQTPTPPSEHVAGLPAPIEKIVMRLLEKEPDDRFQSAGELANAL
                     GAALGDQLQITTPPPFAPGAPKPALIVPTEHSLPPRRSRGVVAGILGVTLVAGAIIAV
                     VLTRTGEHSTAAPPPRDPPPVQVQQAPPPVNVERAPAPVEQPVPVEQPVVPPPAKLEQ
                     VKQPAVKREKPRRDQNGSGSQPRYTKDGSPIEIDIN"
     gene            6421..6792
                     /locus_tag="VFV99_24965"
     CDS             6421..6792
                     /locus_tag="VFV99_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5062657.1"
                     /translation="MPSFQRHVFVCINERPADHPKGCCKAKGGVEVRDRLKAELSSRG
                     ISKLVRANNAGCLDQCEHGVTVVVYPEQVWYGGVTVDDIPELVDKHLVGGEAVERLMI
                     PNQPHLGETRAFAKLRTEKTK"
     gene            6789..7430
                     /locus_tag="VFV99_24970"
     CDS             6789..7430
                     /locus_tag="VFV99_24970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062658.1"
                     /translation="MKRVAIASLLVAIAHVAHADTPQKPGDPPKPAETAAKQPAPDEI
                     ADIESREANLESNEPRKGFTFAGAVGGSISLGDGVGRGPALSLRLGHVATRKTVITFE
                     LGGSSSLHKRATTDETLTDSNFGLMTGAQRYTSGSFWVRAAGGVTFLVKNAMTNGTGG
                     DDAIIGAGGLVGAGLDLARWGYLVLGLEGFGMASASSDGMRAQLAFQLGLSYY"
     gene            complement(7594..9336)
                     /locus_tag="VFV99_24975"
     CDS             complement(7594..9336)
                     /locus_tag="VFV99_24975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5062659.1"
                     /translation="MAQRWCAYAIAAVLAGAIGCGDKQRDVPAAAPVLTKPSVPAAIT
                     SAKIAGTVVDQRSGKPVGNVDVVLLGATEQKARAGADGRFELTVTPGIYRAFVRGDSV
                     LTVGLADRTRLDNAPRAEMAGVRDEALLPMLDARAPLDDVELTVVPTATLAGFVFDEA
                     QHPVANAVVRTRDFDRLDAVRPVLGTDVAVTDEAGHFMLRVPPGHYQVDATHPQFAGT
                     RDGIDIELEAGKPSAITITMARGCIVSGRVVNADGSPATDGAVELRGPRGGFGPAGRL
                     DAGSFRWVTTEDDTVTLRAWPWRSPPSNEKTFECRDGKRFTDVVLRLPDQDPDLSGTI
                     VDANEQPVPLAYLDMQPLDPGLPGQQERGDAAGEWGVFDVPPARYRLTASAPGLGIVD
                     TMTTAPRRDLRLVLGGTGRIVGTTTDLASGSVEVSFLRCGSKDDPLRIAHEPRIVPVR
                     AGHFTIERAPACTLSLAVRWRDTLVESTAVVEPNRTAYIDVDIGTPREKTVTGTVRDA
                     AGSAVEGARVTAVLHDREAATARTDSSGHYTLQTHSGAQLVAGKGEHTGHGAVGQANV
                     ASEQVDLVLDDTGY"
     gene            complement(9355..10128)
                     /locus_tag="VFV99_24980"
     CDS             complement(9355..10128)
                     /locus_tag="VFV99_24980"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5062660.1"
                     /translation="MKLLSVAKTDVGRRRQINEDAFFRDDARGFYVVADGVGGHNKGE
                     IASREAVDQLRSWVYGAQRDLDRLVERVAMGETDCVWEIRRLLESGVKSACYMVYGMA
                     ELDPEKKGMSTTLSALLIRAGYAFAVHVGDSRVYRVRKNNVLQLTEDHTLINYKVKHG
                     MMTKAEAEKATGKNVITRAVGHKDYVQVDTADVDVAIGDRFLLCSDGLHGYFADGDKE
                     VAQLCGDGDLDECAEACISLANQRGGKDNITALVVEVLT"
     gene            10190..11026
                     /locus_tag="VFV99_24985"
     CDS             10190..11026
                     /locus_tag="VFV99_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062661.1"
                     /translation="MSEGPQLAAVLRERSSEILAGWIIRFERSSLRFRRATKAATHTA
                     QVASLVESLTEAVIGGGANALKPGSDATRELERSCAFMGAQFASEGATGFDVAALLLE
                     LREVLAGMVNDADSKVLTHLFEWLTVVALDSFSASGLQSLREQTSEHLDSGTPVIEIL
                     PKVPAVLLVGAPSSSVIDSLLARAWMLAVGTGAPCLIIDCAGIASVGEKHFERGYRGF
                     LEQAEGSALQVLLSGARRPLRELAAKLTSESGLSFQHFDRFDSAVAHALERAGHLLMR
                     RG"
     gene            11059..13302
                     /locus_tag="VFV99_24990"
     CDS             11059..13302
                     /locus_tag="VFV99_24990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062662.1"
                     /translation="MGDFPISYGRYQLVERLAMGGMAELFVATAPGEHGFQKKVVIKR
                     LLPHLSADDTYKAMFIDEAKLTAKLVHPKIAQTFELGRVDDSLFIAMEYVEGIDVLAL
                     LREFAARRRRVEPKLAAWIAHEVLDALDYAHNIRDEGGKQLGIVHRDISPSNVLLSAR
                     GDIKLVDFGIARAIDPDRAHKSKSGTLKGKYGYMSPEQVIEMPLDGRSDVFSVGVVLA
                     ELLTGRRLFAAPNELDVLLMVRDAKLGRLDKYGADIEPNLNVIVRTALKKPLEERWQN
                     AAAFREALGEWLFDQRHRMTPKLIADVVADLRDAVNARRVAAAQGEQVAPTAVIDEVA
                     KSRVPSENLDSIPKDDIDAGESMPIIDVSYDADTSEPPQRAISVSDLAIPRTMTPRGD
                     SGAVRGKPASVQPANKSQPISVVAIGKAANQSGPLKQDSKFDPSQLDNSGVIEVPIAD
                     TISAAVEAIVLPDDRDESSVHSFDDLTEDALPSRSRDIKLPTPEEVARKKPPTPPALS
                     DIADAPDDAGDFSATSPLRVLFRLMTARATGLLVVAVGGIKKEIYVRDGQPEYVSSNV
                     ASELFGNYLVTKGVLSDGELAMALAMMPHYGGKLGDTLVGLGLLKPLEVFRHLTRQVR
                     TKIIDVCTWNKGGFAWYAGRENPREAFPLDFNAFEILGAGAMALAEEVVDSWVAKHGT
                     AKLKASRTRRVGPERFEVKGLVELFEELDGKRTVNALVERGPNREDKMRTARMLCLLE
                     ACDLARA"
     gene            complement(13299..13937)
                     /locus_tag="VFV99_24995"
     CDS             complement(13299..13937)
                     /locus_tag="VFV99_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062663.1"
                     /translation="MRTTILMLIVLSGTAHADRRTDTKPALDEFREYGWLGKPEPFDP
                     RVSPTTARWISIATTVGGLGLSAYLWHHASELGPEHGEAEMQLASIGTGLLTMGLGPA
                     SGLIVAGEYRRGVTGGLTRPFLIAAGGLGMGMGAFVVMQGCFETNDCTGAKIAGGALF
                     GASALVAAAGIGWGIYDIWDTPRILRKRMPAGAVVAPLVGDDRIGISLAFTR"
     gene            14069..14791
                     /locus_tag="VFV99_25000"
     CDS             14069..14791
                     /locus_tag="VFV99_25000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062664.1"
                     /translation="MFIGHYGAAYAIKRWRPDISIFTLFMAVQLLDVAWAVLVFAGVE
                     KVRIVPDITETNSLDLYYMPYTHSLLAAVVWAMASGVTYKALHRAALGAGIAIGLAVF
                     SHWAFDFLVHRPDLPLYDNVKKVGLGWWNFPRFALLLEVGILLGGLVLYLRGTRGVGV
                     LGSIGPWLFVIVLIAVQASVFFGRSISSPNQAAVIALVSYVVFALIAVWLDHHRRVLG
                     NVTPGRPAPVLRGSGAAAAHRP"
     gene            complement(14727..>15102)
                     /locus_tag="VFV99_25005"
     CDS             complement(14727..>15102)
                     /locus_tag="VFV99_25005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062665.1"
                     /translation="ITDARPVSRDSDAPLVQRGDHVRVDHTDGLPAYTAFTVERATDQ
                     YALVRCAAQTGRMHQVRAHLAHVGSPIAGDTLYGGKPLFDLDGFFLHAARISFPLGTE
                     KITVDAPLPHRFREALVRAGLA"
BASE COUNT         2393 a         4986 c         5088 g         2635 t
ORIGIN      
        1 gtcgaggtcg tcgccgtgta catcgcgttg ccggagttcg cgagcacgat gtcacgcgag
       61 aacgcttggc ctttgggaat cgtgccgatg tcgagtaccg gcgggttacc ggtcggcgtc
      121 tgcaccgaca tcacgccacc ggttcccgtg ccggtcaacg acaccgtatc cgtgccgccg
      181 ttcgcggacg ttaccgtcgc gaggacattc ttcgcgccgt gcgtggtcgg cgcgaacgtg
      241 atcgacacga aactgcccga accggcaccg aacgagcacg gactcgccgc ggtgcacgcg
      301 ctcgcgctcc actggttcgc gttcgtggtg gcaccaaaat cgatcacgac gttgttcagc
      361 gtgccgaacc cggtgttctc gacgtagatc atctgcgagg gtgtctggcc gactggcacg
      421 tcgctgaaat cgaggctgtc cggcgtcacg ttcaagaccg ggttggagct cgtcgacgtg
      481 cacgtcacat tcgccatcgc cgggctgccg ttactcgctt cgaacacctg cagcgtggcg
      541 atcgtctgca tcggcccggg cacgcactcg acggtgagcg agtagggcaa gacctccggc
      601 gggctccagt tgcacgtggt cagtccgcag tacgacagct tcatcgacga gtcagtgctg
      661 aaaatcgccg cactaccgag ctgacccgtg tccatcgtga tcaccgtcgc agttgcctgc
      721 gagagcacgc cctgcggccc ggtgatcgcg atcgtgggct ccatgatgtc gatcgtcgtg
      781 atcggtggtg cgtcgggaat cccgccaccg tccgagccga ccggcccgtc gaggccgccc
      841 tccatcggcg cgtcgaagcc accttccatc ggcgcgtcga agccatcgct cggtccatcc
      901 gaaccggcga gcggtgtgtc atcgatcgtt ccaccatcgc caccgatcgc gcccaagacc
      961 acgacagcac gcgcgccata cggcgacgga tcgccatgca cgacgagatc gagcgacgcg
     1021 gatgcagctc cagcagtcga tacgaccact gcggccgcga gcaggcgcgc ccggcttcgc
     1081 ctcatctctc gctagggtat ccgaaccctc gggcggcgtc gtcgggagaa actcggagat
     1141 cggcgtggga acagcacgca catgagcgcg agcacgatcg gcaagatgcc gccgggtcgg
     1201 gtcgacttgc acgagcaggc atagaagctc gtgtcgtcgg tgccattact gcccattccg
     1261 gcatcgggtt ccatgatgtc ttgcgacgtg ccctcgccgc gcagcatgac ttccagcggc
     1321 gcgccgggaa gctgcggcga cgtgatccgt agaacggttt cgaccacgcc aatctgcgtc
     1381 gggtggaagc caacgctgaa cgccgccgtt tcgttcgggt tgagcaccga cggtagcgga
     1441 ctctcgagct tgaacggagt cttgatcatt ggctcgtcga gctgcagcgg cgtgggattg
     1501 cagttctgga tcatcacgcg ctgaccgtct tcgctgtaga ggtggacgag ggccttgccg
     1561 aagtcgagcg cgggaggtgc gatcgcgccg ccctgatcga tgaagcgcgc ggtgatcgcg
     1621 gtcgtcgcgg tttccattcc ggcgacatcg gtgtgccacg cgagcgtgtc gtcgagctgc
     1681 acgaccgcgg tctggcgatg cggcgtgatc gagaccgtcg cggccgcgcc tgcggcgatc
     1741 acggtcgggt acaccgacgg cgacgtggtc tcgagctcga acggtgacgg gacctggccg
     1801 agcgtcggtt gcatcagcgc gatcgtcgcg gtgccgtcgg acacgagcga aacgttggag
     1861 gacgtggtcg gctggccgac gcagaacgtc ccgagatcga gtgcgttggc cgcctcgtag
     1921 cccgcacgca cgactttgcc gctgagcgga atccgcagca tttcgccagc ggtgtcggta
     1981 acgatgatcg ctccggtcat ctgaccttcg tcggcgtcgg cgggcggatc gaggacgacg
     2041 tcgaacgtcg cgggcgtcgt gagctgcgac agcgcgccga gcgtgaggcc gggcgcgttg
     2101 ccctcgacct gcatctggct gttgagcgtt agctggctcg gggcgttcgt gttgagtagc
     2161 tggatcgtgc gccggacttc accgccgccg gcgcggacct cgcccaggtt cagtgcagtc
     2221 ggattcgcga acaggtcgag cgtgctgcct tcgcaggtcg cgttcagcgt cgtcggcgat
     2281 ccgttcaccg cgtccatcgc cgtggccgtg accgacgtca tgaactggct cgcggtcgtg
     2341 ggtgcgcagg tcagggtgac tgcgcggtcg acgtttgcca tcacgaccgt cgacgcggcc
     2401 ggcgacagcg tgaacggcgg cgtcgagctt gcggcaagcg cgaggttcac gtcgagatcg
     2461 aggttgccgt gattcgtcag cgcgaagtcg atcgccgacg agcgtccgac cgggaccgag
     2521 ccgaatacga gggtcgaaac atcaccggcg acgtcgagcg tgccgccgat gctcctgccg
     2581 tcgagcggaa tctccttcgt gcgatcgatc gaatccttgt acgaaacgag cagcgtcgcg
     2641 cgacgacgac cgatgccgct cggatcgaac gtcaggttga tgtcgacctg ctcgccgggc
     2701 tccagtgagc acgacatcgt gatctgaccg ctgcactcgc cgctcgcggc gtacgtccag
     2761 tcgttcgccg cgccggcgtc gacatcgacg gtgcgcacgt cgtggatcac gagcgaaccg
     2821 gcgcccgcgt tcgtcaggtg aaccaccgtg ctcgcgctgc cgctggcggc atcgaccggc
     2881 gtcggcaacg agatattcgt cgggttgaga ccgagcaccg gcacggtcgt ggcggctccg
     2941 ttgcacgtaa gcgtcacggg ctgcgcgagc agctggaacg tgttcgtgcc gatgtacagc
     3001 tgcgcggtgt gcaggcccgg cgtttgcggc gtgcacttca cctggatcgg cagcgtgcca
     3061 ccctggccga tcgccatcac gtcttcatcg cagaacggcg cgtcgggatt gcacgggttg
     3121 gagaactgga agttgccgcc gaggtcgtcc gtttgcagat agacgcgcgt gatgaactgg
     3181 ccgagcgtgc cgttgttccg cacgtcgatc tgttgcatcg cgttgtcgcc cacggtgacc
     3241 gtgccgaaat cgatcgtcgt ctgctgcggt gccagcgttc cgggcgcgtt gccgtacaga
     3301 cacacggtca tgaagtcggc gagtgcggtg ccgttcgtgt tgttcgtcgc gtgatagagg
     3361 cagcgccgca tcgcaggcgt gccgcgcgcg ggacatgtga ggttcacgtt gcgcatcgtc
     3421 gtcggcgaga tcacgaccgg gttgccaccg acgacgctga acatcagcga tgggtcgcag
     3481 ctcgcatcgg aaccgacgag cacgctgtac gtcgtagcgc ccgtcgtgtt ctgcagcgac
     3541 gcggtgccgg tgcccgtgcc ggtcgcgctc gtgatggtga taaccgtgac ctccgggttc
     3601 gcgcggatgt caccattgcc gaccgcatac gcgacgagcg cggcaccgct gacgagcgcg
     3661 accgcaattc cacgcacgag catgcgacgc ctggtaatca caaccgcccg atgacgccga
     3721 cggtcggcac gaacaccgtt tcgtcggggc gcttgccgtg gacgagcgcg tccttgacgt
     3781 tgaagaagtg ctcgatcccg acatcggcct ggatgctgac gtgaccgttc agcttgagct
     3841 cgacgccacc tgcgccacgc aggccgagcg cgatccgcga ctccatcgtg gtctcgtcgg
     3901 tatagaacca gagcggcacg ccaccgccgg cgtagggccg tagccagccg gtcaaaaatc
     3961 ggatgcgcat tccaaagtac acgccccagt cttctgcctt gagaccagcc agctcgagct
     4021 cgacgctgtc gagcggtgag agcgcgatgc cgaggccgcc cgcaacgccg cggcctttgc
     4081 cgtcgacgcg caagacgccg agcacaccgg aggtgatccg tcgctcgctc ggcggcggcg
     4141 ccacggtgac gctgccgtca tcttcgatcg tgtagtgcgg cacgatcgga cgcattaccg
     4201 gaccgccata gccggcgagc acgtgacgct cgccgccgct cacgttctcc gtcgcatact
     4261 gccggccggt gatcagccag ccgtcgacgt tgtcgggtgc aggctcggga ggcttcggcg
     4321 gcgtcgcggc cgcggtcggg ttcgtcgggg gcgtcggatt tgtcggattc gtcggcggcg
     4381 tcgggctcgg cgccgccttc ggctcgacct tcgtcgtgat gtctttgctc tcgccttcaa
     4441 agccgttgat ggtgagctcg gtcgtcgcat cacctttgcg cacgcgcacg agatggatgc
     4501 cggcgcgcac gcgcgtctgc agcgtcgagc cgactggaat gaacgggccg gcgtcgatcg
     4561 atacttcgga gccgcgcggg aacacgcgga gcgtcaatac cgtgagctct tggtcgagct
     4621 tgacgagcag ctccttgacc tcggcgatgc gctcgggacc ggttgccgga tccgcgaggt
     4681 agcgctggta cgtgttcgcc gcgtcggcga gccgccccat gtcgcgcagc gtggagccga
     4741 tgttgagcaa gatcttcggg ctcgggaact tgccgtacgc ttcgaggaag ttcgcgagcg
     4801 cctgctcgta gccctgcgcc tgcagctggc gcacgccttc cgtcatgagc gctgctgcga
     4861 gtggccggtt cttctcgtca tcgacgcgga tcgccggatc ctgcgtgatc gtcgcgagcg
     4921 cttcggcgag ttgcttctcg tcgcgaatct gcgcgagcgg tgggaccgca tcgggattcg
     4981 tgaccggctg cgcataggcg gcggacgccg agagcgctag cgcgatcgag agcctgcctc
     5041 gcatctggcc ctagtttatg tcgatctcga tcggcgatcc gtctttggtg taccgaggct
     5101 gcgatccact gccgttttga tcacgccggg gtttttcgcg tttgacggcg ggctgcttca
     5161 cctgttcgag ctttgccggt ggggggacga cgggctgttc cacgggaacg ggctgttcca
     5221 ctggtgccgg cgcccgctcg acgttgaccg gcgggggcgc ctgctggacc tggaccggcg
     5281 gcggatcacg gggcggcggg gcggcagtgg agtgctcgcc ggtgcgcgtc agcacgaccg
     5341 cgatgatcgc accggccacc aacgtgacgc cgaggatgcc ggcgaccacg ccacgcgagc
     5401 gacgcggcgg cagcgagtgc tcggtcggca cgatcaacgc gggcttcggt gcaccgggcg
     5461 cgaacggtgg cggcgtcgtg atctgcagct ggtcgccgag cgcggcgccg agcgcattcg
     5521 cgagctcgcc tgcgctctgg aaccgatcgt ctggttcctt ctctagcagg cgcatcacga
     5581 tcttctcgat cggcgcgggc agcccagcta cgtgctcgct cggtggcgtc ggcgtctgga
     5641 acatctgcag cgcgatgatc tcgccggcgc cgactgcgac gaacggcggc tgcccgcaga
     5701 gcatctcgta gagcatgcag ccgaccgaat acagatcggc gcgatgatcg atcccgctcg
     5761 ctgcacgcgc ttgctcgggt gccatgtaca gcggcgtgcc gatcagcgcg ccggtctgcg
     5821 tgtgatggtt cgtgtcggag agcttcgcga tgccgaagtc gagcaccttc ggacgctcgc
     5881 cgagatcagg atccggaatc aggaacacgt tgtcgggttt gagatcgcga tgaacgatgc
     5941 ccttcgcgtg cgcggcggtc agtgcggccg cgatgtcgcg cgcgatcttt gcggcctcgc
     6001 gtggcgatag ccgcccgcgg ctctcgatgc gtcgggcgag cgactcgccc ttgagcatct
     6061 ccatcacgaa gtaggcgcgg ccgtcgctcg cgtggccaaa gtcgagcacc tcgatgatcc
     6121 cgggatggcg gatcgcgctc gcggcctttg cttccgtgat gaagcggctg accagctcgt
     6181 cgttcgtggt cagctccgga cgcaacagct tgatcgcgac cgtgcgttcg agcacggagt
     6241 gttcggcccg gtacaccttg cccatgccgc cgcggctcag ctcgccggtg atccggtagc
     6301 tgccgagcat cgtgccgacg agattcggct cctccatccg cccttcgagg atgcgccaac
     6361 cctcacgccg gggcaacgcg cgtcgggtgg gcggaagtag gcgtgctagc ctcgcgcagg
     6421 atgccgagtt ttcagcgcca cgtgtttgtc tgtatcaacg agcggccggc cgaccacccg
     6481 aagggctgct gcaaagcgaa gggcggcgtc gaggttcgcg accggctcaa ggccgagctg
     6541 agctcgcgcg gcatttcgaa gctcgtgcgt gcgaataatg ctggctgcct cgatcagtgc
     6601 gagcatggcg tcaccgtggt cgtgtatccc gagcaggtct ggtacggcgg tgtgaccgtc
     6661 gatgacattc cagagctcgt cgacaagcac ctggtcggcg gcgaggcggt cgagcgcttg
     6721 atgattccga accaaccgca cctcggcgaa acgcgcgcct ttgcgaagct gcgcacggag
     6781 aagacgaagt gaagcgcgtc gcgatcgcga gcctcctcgt tgcgatcgcg cacgtcgcgc
     6841 acgccgatac gccccaaaaa ccgggcgacc cgccgaagcc tgccgagaca gctgcgaagc
     6901 agccagcgcc cgacgagatc gccgacatcg agagtcgcga ggcgaacctc gaatcgaacg
     6961 agccgcgcaa aggcttcacg tttgcgggtg ccgtcggcgg cagcatctca ctcggtgacg
     7021 gcgtcggccg cggaccggcg ctgtcgttgc ggctcggcca cgtcgccacc cgcaagaccg
     7081 tcatcacgtt cgagctcggc ggtagcagct cgctgcacaa gcgcgcgacc accgacgaga
     7141 cactcaccga cagcaacttc ggcctgatga ccggcgcaca gcgctacacg tcgggctcgt
     7201 tctgggtgcg cgcagcaggc ggcgtgacgt tcctggtcaa gaacgcgatg acgaacggca
     7261 ccggtggcga cgacgcgatc atcggtgctg gcggtctcgt cggcgctggc ctcgatctcg
     7321 cgcgctgggg ctacctcgtg ctcggcctcg aaggcttcgg catggcatcc gcgtcgagcg
     7381 acggcatgcg cgcgcaactc gcgttccagc tcgggctctc gtactactga acgctgcggc
     7441 gatcgcgacg agcctctcgt cgagaacgcg cgcgaatccg gggcgccgac gtgtccggga
     7501 gtgtccaacg taaccccggt acgtttgcgg caacgtcgca gaatggctcc tgtccggctg
     7561 tccgaattcg tccggttttg tccggcgcgt tgttcaatag ccggtgtcgt cgaggacgag
     7621 gtcgacttgc tcgctggcaa cgttcgcctg gccgacggcg ccgtggccgg tgtgctcgcc
     7681 cttgcccgcg acgagctgcg cgcccgagtg cgtctgtagc gtgtagtgcc ccgatgaatc
     7741 ggtgcgcgcg gtcgcggctt cgcgatcgtg aaggaccgcg gtgacacgcg cgccctcgac
     7801 ggcggaaccg gcggcatcac gcacggtgcc ggtcacggtc ttctcgcgcg gcgtgccgat
     7861 gtcgacatcg atgtacgcgg tgcgattcgg ctcgacgacc gcggtggact cgacgagcgt
     7921 gtcgcgccag cggaccgcga gtgacagcgt gcacgccggc gcgcgctcga tcgtgaagtg
     7981 gccagcgcgc accggcacga tacgcggctc gtgcgcgatg cgcagcggat catctttgga
     8041 gccgcagcgc aggaacgaga cctcgacgct gccgctcgcg agatcggtcg tcgtgccgac
     8101 gatgcggccg gtgccgccga gcacgagccg cagatcgcgg cgcggtgcgg tcgtcatcgt
     8161 atcgacgatg ccgagcccag gcgccgacgc ggtgaggcga tagcgtgcgg gcggtacgtc
     8221 gaagacgccc cactcaccgg cggcgtcgcc gcgttcctgc tggccgggca gaccgggatc
     8281 caggggctgc atgtcgagat atgcgagcgg caccggctgc tcgttcgcgt cgacgatcgt
     8341 gcccgacaga tcgggatctt ggtcgggcag ccgtagcacg acgtcggtga accgtttgcc
     8401 gtcgcggcac tcgaacgtct tctcgttcga cggcggcgac cgccatggcc acgcgcgcag
     8461 cgtgacggtg tcgtcctcgg tcgtcaccca gcgaaacgag ccggcatcga gccggcccgc
     8521 cgggccgaac ccaccgcgcg gaccgcgcag ctcgaccgcg ccgtcggttg ccggcgagcc
     8581 atccgcattg acgacgcgcc cgctgacgat gcagccgcgc gccatcgtga tcgtgatggc
     8641 gctcggcttg cccgcttcga gctcgatgtc gatgccgtct cgcgtgccgg cgaattgcgg
     8701 gtgggtcgcg tcgacctggt agtggccggg cggcacacgg agcatgaagt gcccggcctc
     8761 gtcggtcacg gcgacatcgg taccgagcac cgggcgcacc gcgtcgagcc ggtcgaagtc
     8821 gcgcgtgcgt acgaccgcgt tcgcgaccgg atgttgtgcc tcgtcgaaca cgaagccagc
     8881 gagcgtcgcg gtcggcacca cggtgagctc gacgtcgtcg agtggtgcgc gtgcgtcgag
     8941 catcggcagc aacgcctcgt cgcggacgcc ggccatttcg gcgcgaggtg cgttgtcgag
     9001 ccgcgtgcga tcggcgaggc cgaccgtcag cacgctgtcg ccgcggacga acgcgcggta
     9061 gatgccgggc gtcaccgtga gctcgaagcg gccgtccgcg ccggcacgcg ctttctgctc
     9121 ggtcgcgccg agcagcacga cgtcgacgtt gccgacgggt ttgccgctcc gctggtcgac
     9181 cacggtcccc gcgatcttcg cgctcgtgat cgcagcgggc acgcttggct tcgtcaggac
     9241 cggcgccgcc gctggaacgt cgcgctgctt gtcgccacag ccgatcgccc cggctagaac
     9301 cgccgcgatc gcgtacgcac accagcgctg tgccatctcg atcgagatag cacgttacgt
     9361 cagaacttcg acgacaagcg cagtgatgtt gtctttgccg ccgcgctggt tcgcgagcga
     9421 gatgcaggct tccgcgcact cgtcgaggtc gccatcgccg cacagctgcg cgacttcttt
     9481 atcaccgtcc gcgaaatagc cgtgcaggcc gtcgctgcag agcaggaacc gatcgccgat
     9541 cgcgacatcg acgtcggcgg tatcgacctg cacgtagtcc ttgtggccga ctgcgcgcgt
     9601 gatgacgttc ttgccggtcg ccttctcggc ttcggccttc gtcatcatgc cgtgcttgac
     9661 cttgtagttg atgagcgtgt ggtcctcggt cagctggagc acgttgttct tgcgcacgcg
     9721 gtacacgcgc gagtcgccga catgcaccgc aaacgcataa cccgcgcgaa tgagcagcgc
     9781 cgagagtgtc gtcgacatgc ccttcttctc ggggtcgagc tcggccatgc cgtagaccat
     9841 gtaacaggcg ctcttcacgc cgctctcgag aagtcggcgg atttcccaca cgcagtcggt
     9901 ctcacccatc gcgacgcgct cgaccaagcg gtctaagtca cgttgagccc cgtagaccca
     9961 cgaccgcagc tgatcgacgg cttcgcggct cgcgatctcg cccttgttgt ggccgccaac
    10021 tccgtccgcc accacgtaga agccgcgcgc atcatcccga aagaaggcgt cctcattgat
    10081 ctgacgccgg cgcccgacat ctgttttggc gacagacagg agcttcaaag tgccacccgt
    10141 tgcgcatcct aacatggcgg cacgcaaatc cgcttgctag gctgatttgg tgagtgaagg
    10201 gccccagctc gctgcagtgc tgcgtgagcg atcaagcgag atcctcgcag gatggatcat
    10261 ccgcttcgaa cggtcttcgc tccggttccg ccgcgccacc aaagcggcca cgcacaccgc
    10321 gcaagtcgcg agcctcgtcg agtcacttac tgaagccgta atcggtggcg gtgcgaacgc
    10381 cctcaagccc ggcagcgacg cgacccgcga gctcgagcgc agctgcgcgt tcatgggcgc
    10441 gcagtttgcg agcgaaggtg ccaccggctt cgatgtggcc gcgctgctgc tcgagctgcg
    10501 cgaggtcctc gccggcatgg tcaacgacgc cgattcgaaa gtgctgacgc acctgttcga
    10561 gtggctcacg gtcgtcgcac tcgattcgtt ctcggcctcg ggtctgcaat cgctacgcga
    10621 gcagacgtcc gagcatctcg attccggtac gccggtgatc gagattttgc cgaaggtacc
    10681 ggccgtcctg ttggtcggcg cgccgagctc gtcggtcatc gacagcttgc tcgcgcgcgc
    10741 atggatgctg gcggtcggca ctggtgcgcc gtgcctgatc atcgactgcg cgggcatcgc
    10801 gtcggtgggc gagaaacatt tcgagcgcgg ctatcgcggc tttctcgagc aggccgaagg
    10861 cagcgcgctg caggtcctac tctcgggcgc gcggcggcca ctccgcgagc tcgcggccaa
    10921 attgaccagc gagagcggcc tctcatttca gcacttcgat cgttttgata gtgccgtggc
    10981 acacgcgctc gaacgcgcgg gtcacttatt gatgcgtcga ggctgagagc tccgcactcg
    11041 tgataggctt ttggctcggt gggagacttc ccgatcagct acggccgcta tcagctggtc
    11101 gagcgtctcg ccatgggcgg gatggccgag ctgttcgtgg cgacggcacc gggagagcac
    11161 ggctttcaga agaaggtcgt gatcaaacgc ctacttccgc atctgtctgc ggacgacacg
    11221 tacaaggcga tgttcatcga cgaggcgaag ctcaccgcca agctcgtcca cccgaagatc
    11281 gcgcagacat tcgagctcgg ccgcgtcgat gactcgctgt tcatcgcgat ggagtacgtc
    11341 gaaggcatcg acgtgctcgc gctgctccgc gagtttgcag cgcgtcgccg ccgcgtcgag
    11401 cccaagctcg ccgcctggat cgcacacgag gttctcgacg cgctcgacta cgcgcacaac
    11461 atccgcgacg agggcggcaa gcagctcggc atcgtgcatc gcgacatctc gccgtcgaac
    11521 gtgctgctca gcgcgcgcgg tgacatcaag cttgtcgact tcggcatcgc tcgtgcgatc
    11581 gatccggacc gcgcgcacaa gtccaagtcg ggcacgctca agggcaagta cggatacatg
    11641 tcgcccgagc aggtgatcga gatgccgctc gatggccgta gcgacgtgtt ctcggtcggc
    11701 gtcgtgctcg ccgagctact cacgggccgc cggttgttcg ccgcgccgaa cgagctcgac
    11761 gtgctgctca tggtgcgcga cgcgaagctc ggccgcctcg acaagtacgg cgccgatatc
    11821 gagcccaatc tcaacgtgat cgtgcgtact gcactcaaga agccactcga ggagcgctgg
    11881 cagaacgccg ccgcatttcg cgaggcgctc ggcgaatggc tgttcgatca gcgccaccgc
    11941 atgacgccga agctgatcgc cgacgtcgtc gccgatttgc gcgatgcggt gaacgcacgc
    12001 cgcgtcgcag ccgcacaagg cgagcaagtc gcgccgaccg cggtgatcga cgaagtcgcg
    12061 aagagccgcg tgccgagcga gaacctcgac tcgatcccga aggacgacat cgacgccggc
    12121 gagtcgatgc cgatcatcga cgtcagctac gacgccgaca cgagcgagcc gccgcagcgg
    12181 gcgatctcgg tcagcgatct cgcgattccg cgcacgatga caccgcgcgg tgactcgggt
    12241 gcagtgcgcg gcaagccggc gtcggtgcag ccggcgaaca agagccagcc gatctcggtg
    12301 gttgccatcg gcaaggccgc aaatcagagc ggcccactca agcaggactc gaagttcgac
    12361 ccgtcgcagc tcgacaacag cggtgtgatc gaagtgccga tcgccgacac gatcagcgct
    12421 gcagtcgaag cgatcgtgtt gcccgacgat cgcgacgaga gctcggtgca ctccttcgat
    12481 gatctcaccg aggatgcgct gccgagccgc tcgcgcgaca tcaaactgcc gacccccgaa
    12541 gaggtcgcgc gcaagaagcc gccgacgcca cccgcgctct ccgacatcgc cgatgcaccg
    12601 gacgacgccg gtgacttctc cgcgacgtcg ccactgcgcg tgctgttccg cttgatgacc
    12661 gcacgcgcga ccggcctgct cgtcgtcgcg gtcggtggca tcaagaaaga gatctacgtt
    12721 cgcgacggcc aacccgagta cgtgtcgtcg aacgtcgcga gcgagctgtt cggcaactat
    12781 ctcgtcacca agggcgtgct gtccgacggc gagcttgcga tggcgctcgc gatgatgccg
    12841 cattacggcg gcaagctcgg tgacacgctc gtcggcctcg gtctgctcaa gccgctcgag
    12901 gtcttccgcc acctcacgcg gcaggtccgc acgaagatca tcgacgtctg cacgtggaac
    12961 aaaggcggct tcgcttggta cgcaggccgc gagaatccgc gcgaggcgtt cccgctcgac
    13021 ttcaacgcgt tcgagatcct cggtgccggc gcgatggcgc tcgcggagga agtcgtcgac
    13081 agctgggtcg ccaagcacgg cacggccaag ctgaaggcat cgcgcacccg gcgcgtcggt
    13141 cccgagcgct tcgaggtcaa aggcctcgtc gagctgttcg aggagctcga cggcaagcgc
    13201 accgtcaacg cgctcgtcga acgtggaccg aaccgcgagg acaagatgcg caccgcgcgc
    13261 atgctctgcc tgctcgaggc ctgcgatctc gcgcgcgcct aacgcgtaaa cgcgagcgaa
    13321 atcccgatgc ggtcatcgcc gacgagcggt gcgaccaccg cgccggcggg catcctctta
    13381 cgcaagatgc gcggcgtatc ccagatgtcg tagatgcccc agccgatgcc ggccgctgcg
    13441 accagcgcac tcgcgccgaa caacgcgcca ccggcgatct tcgccccggt gcagtcgttc
    13501 gtctcgaagc agccttgcat gacgacgaat gcacccatac ccattccgag cccgccggcc
    13561 gcgatgagaa acggtcgcgt gagcccgccg gttacgcccc tccgatactc gccggcgacg
    13621 atcagcccgc tcgccggtcc gaggcccatc gtcaacagac cggtgccgat gctcgcgagc
    13681 tgcatctctg cctcgccgtg ctccggtccg agctcgctgg cgtgatgcca caaatacgcg
    13741 ctgagcccga gaccgccaac ggtcgtggcg atcgagatcc agcgcgcggt tgtcggtgat
    13801 acgcgcgggt cgaacggctc gggcttgccg agccagccgt actcgcggaa ctcgtcgagc
    13861 gcgggtttcg tgtcggtgcg gcgatccgca tgcgcggtac cggagagcac gatcagcatc
    13921 aggattgtcg tgcgcacgct cagagcgtag gcgatgcgca cggcgtggcg ggctgcgacg
    13981 tacttgcttt ggatgacgag tcgctgctac gcgctcgtcg cattggtgtc gcagtccggc
    14041 ggcgcacgca ttgcaacgtg ttccccgtat gttcatcggg cattacggtg cggcgtacgc
    14101 gatcaaacgc tggcgtcccg acatctcgat cttcaccctg ttcatggcag tccagctcct
    14161 cgacgtcgcg tgggcggtgc tcgtgttcgc cggcgtcgag aaggtccgca tcgttcccga
    14221 catcaccgag acgaactcgc tcgacctcta ttacatgccg tacacgcaca gcctcctcgc
    14281 tgcagtcgtg tgggcgatgg ccagcggcgt gacgtacaag gcgctgcacc gcgcggcgct
    14341 cggtgccggc atcgcgatcg gcctcgccgt gttctcgcac tgggcattcg atttcctcgt
    14401 ccatcgcccc gatctcccgc tctacgacaa cgtcaagaag gtcggactcg gctggtggaa
    14461 ctttccgcgc ttcgcgttgc tgctcgaggt cggcatcctg ctcggcgggc tggtgctcta
    14521 cctgcgcggc acccgcggcg tcggtgtgct cggcagcatc gggccgtggt tgttcgtcat
    14581 cgtgctgatc gccgtgcaag cgtccgtgtt cttcggccgc tcgatcagct cgccgaacca
    14641 ggctgccgtc atcgcccttg tctcgtatgt cgtctttgcc ttgatcgcgg tgtggctcga
    14701 tcatcaccgc agggtgctcg gcaacgtcac gccaggccgg cccgcaccag tgcttcgcgg
    14761 aagcggtgcg gcagcggcgc atcgaccgtg atcttctcgg tgccgagcgg gaagctgatc
    14821 cgcgcggcat ggaggaagaa gccgtcgagg tcaaagagcg gcttgccgcc gtacagagta
    14881 tcgccggcga ttggcgagcc gacatgcgcg aggtgcgcgc gcacttgatg catgcggccg
    14941 gtctgtgccg cgcagcgcac gagtgcgtac tgatcggtcg cgcgctcgac ggtgaacgcg
    15001 gtgtacgccg gcaagccgtc ggtgtgatcg acacgcacgt gatcgccgcg ctggacaagc
    15061 ggcgcgtcgc tgtcgcgcga cacggggcgt gcgtcggtga tc
//
LOCUS       DASUFX010000621         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1905525, whole genome shotgun sequence.
ACCESSION   DASUFX010000621 DASUFX010000000
VERSION     DASUFX010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905525"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFV99_25010"
     CDS             <1..486
                     /locus_tag="VFV99_25010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062666.1"
                     /translation="EGAPDDESVQIHRMIPDHPITIWNGCSEKDMEDVLRSIGDAIAI
                     GAPLYNKGTHEGYEACFRVYEGTALKYEKDAACKGVRGAFGDGLLRATSLGTFKEKAW
                     AMRDTFDGLIAAFRRWCEQDQSCMKKFGGPPGGATGGTDLDPKSDKTRKPETKPDTKN
                     K"
     gene            483..1145
                     /locus_tag="VFV99_25015"
     CDS             483..1145
                     /locus_tag="VFV99_25015"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5062667.1"
                     /translation="MTILYLFDIDGTLLHAAGSGRAAFDAVMTDLHGLANASEGIRYG
                     GKTDHAIIDEIFAARLGRASTPAERDAFIDAYIPKLHELLVTKGVEVLGGVVETLEYL
                     QTQPVRLGVATGNVRRGADAKLGAAGLARWFEVGGYGCDSIVRAELVAKAIERGRDGR
                     EVREVVVVGDTIHDISAARACGATVVAVATGADSKQALAHADAVFASMAELPAWHAAR
                     FS"
     gene            complement(1138..2244)
                     /locus_tag="VFV99_25020"
     CDS             complement(1138..2244)
                     /locus_tag="VFV99_25020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5062668.1"
                     /translation="MSSGTVSDFLRSQQGRIALRWEEEVRADLPAKRIKQKPALFDHL
                     PEFLDGLANWIDGNTELAEHAFGRLADSHALQRLGYGVGLETLTCEYSKLRVVIARLL
                     MEVPPTDDSREALVRLHEGMDKAINEAMHRYAMRREEVRERFVAILGHDLRDPLSTVV
                     ISANTLAANPSLKPEHRVVASRIVRACDRMQRMINDVLDFARGHLGTGIPANPTPNDM
                     GEICRAAADEIIGANPQREVAIDLEGDLRGTFDRDRVHQAITNLLANAVNHSEGSIEI
                     RAYESADRQSIITEVTSHGDRIPEELRARLFDPFAQGDIAGPRRGLGLGLFIVQQIAI
                     AHGAQCDVTSDETGTTFSVRWPRSPGLERRVAAS"
     gene            complement(2376..2837)
                     /locus_tag="VFV99_25025"
     CDS             complement(2376..2837)
                     /locus_tag="VFV99_25025"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5062669.1"
                     /translation="MGQAIGTGDKKSVNVELNIIPFIDLMSCLTAFLLVTAVWVNLAS
                     VKNDPAGKAKSDVEQPPAPKLAVLIEHDQILVQATPSGEARQLAAFDWSGLETTLRDL
                     KTPGEVPQVEIAAESSNTHPIAYQQLIAAMDVAVKAGYPRVGVTDPSSLTR"
     gene            complement(2873..>3936)
                     /locus_tag="VFV99_25030"
     CDS             complement(2873..>3936)
                     /locus_tag="VFV99_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062670.1"
                     /translation="RDKRLVPVCIAHPATKTPQCTLVGDKTELPIGAACPAWVDGNAA
                     SSYYTVHWQDRPATQWLARLSPNARVIVGDDVAATFRRGELSAKDALGELRTLFAGDL
                     DTQIAGIALAKEIDAIVDDATRPAWSRWLAPRLPKLKRGKNPQQVYQLARELGPMLVT
                     ADRLAAGERVKARELVDDELLGDRFPDPPMLGALTTGDARSLFDRLVDKALSAREPDR
                     QEHWLGLLGAFPPDFAERTATLLVDKAELPVDPIWSALEEYFERPSARIAAWKALRDH
                     LDVLMKRAAHHGADMIGAAAWLCDQSSRDEVAKAFVPFLDRIPAGKTRLDRSLATIDQ
                     CIARRGRIGDLAAAIATSR"
BASE COUNT          653 a         1269 c         1348 g          666 t
ORIGIN      
        1 gaaggcgcgc ccgacgacga atcggtccag atccatcgca tgatccccga ccacccgatc
       61 acgatctgga acggctgctc cgagaaggac atggaagacg tgctgcgctc gatcggcgac
      121 gcgatcgcga tcggcgcgcc gctctacaac aagggcacgc acgagggcta cgaagcgtgc
      181 ttccgcgtct acgagggcac cgcgctcaag tacgagaagg acgcggcctg caagggcgtg
      241 cgcggcgcgt tcggcgacgg cctgttgcgc gcgacgtcgc tcggcacctt caaagagaag
      301 gcgtgggcga tgcgcgacac gttcgatggc ctgatcgccg cgttccggcg ctggtgcgag
      361 caagaccaat cgtgcatgaa gaagttcggc ggcccgcccg gcggtgccac gggcggtacg
      421 gatctcgatc cgaagtcgga caagacgcgg aagcccgaga ccaagcccga cacgaagaac
      481 aagtgaccat cctctacctg ttcgacatcg acggcactct gctgcacgcg gctggttccg
      541 gacgtgccgc gttcgacgcg gtgatgacgg atctgcacgg cctcgcgaac gcgagcgaag
      601 gcattcgcta tggcggcaag accgaccacg cgatcatcga cgagatcttc gcggcgcgcc
      661 tgggccgtgc atcgacgccg gcggagcgtg acgcgttcat cgacgcgtac attccgaagc
      721 tccacgagct gctcgtgacc aagggcgtcg aggtgctcgg cggcgtcgtc gagacgctcg
      781 agtatttgca gacccaaccg gtgcggctcg gtgtcgcgac gggcaacgtg cggcgcggcg
      841 cggacgcaaa gctcggcgcc gctgggctcg cgcggtggtt cgaagtcggc ggctatggct
      901 gcgactcgat cgtgcgcgcg gagctggtcg cgaaggcgat cgagcgcggg cgagacgggc
      961 gagaggtccg cgaggtcgtg gtcgtcggcg acacgatcca cgacatctcg gccgcgcgtg
     1021 cgtgcggcgc gaccgtcgtt gcggtagcga ccggcgctga ttcgaagcaa gcgctcgcgc
     1081 atgccgacgc ggtgttcgca tcgatggccg agctaccggc ctggcacgca gcacgcttca
     1141 gctagcggcg acgcgacgtt ccaggccagg gctgcgcggc cagcggaccg agaacgtggt
     1201 gccggtctcg tcggacgtga cgtcgcactg cgcgccgtgc gcgatcgcga tctgctggac
     1261 gatgaagagc ccgaggccga gcccgcggcg cggacccgcg atgtcgccct gcgcgaatgg
     1321 atcgaacaag cgcgcgcgca gctcctcggg gatgcggtcg ccatggctcg tgacctcggt
     1381 gatgatcgac tgacggtcgg cgctctcgta cgcgcggatc tcgatcgagc cttcgctgtg
     1441 attgacggcg ttcgcgagca agttcgtgat cgcctggtgc acgcgatcgc gatcgaacgt
     1501 gccgcgcaag tcgccttcca gatcgatcgc gacctcgcgc tgcgggttcg caccgatgat
     1561 ctcgtcggca gcggcgcggc agatttcgcc catgtcgttc ggcgtcgggt tggccgggat
     1621 gccggtgccg aggtggccgc gcgcgaagtc gagcacgtcg ttgatcatgc gctgcatgcg
     1681 atcgcacgcg cgcacgatgc gcgacgcgac cacacgatgc tcgggcttga ggctcggatt
     1741 cgccgcgagc gtgttcgcgg aaatcacgac ggtgctcagc ggatcgcgca agtcgtggcc
     1801 gaggatcgcg acgaaccgct cgcggacttc ttcgcggcgc atcgcgtagc ggtgcatcgc
     1861 ttcattgatc gccttgtcca ttccctcgtg gaggcggacg agtgcctcgc gggagtcgtc
     1921 ggtcggcggt acttccatga gcaggcgcgc gatcaccacg cggagcttcg agtactcgca
     1981 ggtcagcgtc tcgaggccaa ccccgtagcc gagccgctga agtgcatggc tatcggcgag
     2041 ccggccgaag gcatgttcgg cgagctcggt attgccatcg atccagttcg cgagaccgtc
     2101 gaggaactcc ggaaggtgat cgaacagtgc gggcttctgc ttgatgcgct tcgccggtag
     2161 atcggcccgc acttcttcct cccaccggag cgcaatccgt ccctgctggc tgcgcaggaa
     2221 gtcgctgacg gtgccggagc tcatacttgg aacctagcgg acgtttccac acacggcacc
     2281 tcgggaggtt tacgcaaatg atcacgagcg tcgccgcaca tgcgcacgca tgcgcgctcg
     2341 ttcgagcgac gagcgccacg gtcacgccgg caagatcagc gcgtgagact ggacggatcg
     2401 gtgacaccga ctcgcgggta gcccgccttc accgcaacgt ccattgcggc gatcaactgc
     2461 tgatatgcaa tcggatgtgt attcgagctt tcagctgcga tctcgacctg tggcacttcg
     2521 cccggtgtct tcaaatcgcg cagtgtggtc tccaggccgc tccagtcaaa tgcggctagc
     2581 tgacgagctt cgccacttgg cgtcgcttgc acgaggatct ggtcgtgctc gatgagtacc
     2641 gcgagctttg gcgccggcgg ctgctcgacg tcgctctttg ctttgcccgc cggatcgttc
     2701 ttcacgctcg cgaggttcac ccagaccgcg gtgaccaaaa gaaacgcggt gagacagctc
     2761 atcagatcga tgaacggaat gatgttgagc tcgacgttga cggacttctt gtcacccgtg
     2821 ccgatcgctt gacccatgcc ggctagacac acgcgcccga tggttgttgc gcttagcgcg
     2881 aagtagcgat ggccgctgca agatcgccga tgcgtccgcg acgcgcgatg cactgatcga
     2941 tcgtggcgag cgaccgatcg agacgcgtct tgccggcggg aatgcgatcg aggaacggca
     3001 cgaatgcctt cgcgacttcg tcgcgcgatg actgatcgca gagccaggcc gcagcaccga
     3061 tcatgtcggc gccgtgatgc gccgcgcgct tcatgagcac gtcgaggtga tcgcgcagcg
     3121 ccttccaggc agcgattcgc gcgctcggcc gttcgaagta ttcctcgagc gcggaccaga
     3181 tcggatcgac tggcagctcg gccttgtcga cgagcagggt cgctgtgcgc tcggcgaagt
     3241 ctggtgggaa cgcgccgagc aggccgagcc agtgctcctg cctgtcgggt tcgcgcgcag
     3301 acagcgcctt gtcgacgagg cgatcgaaca gcgagcgtgc atcgccggtc gtgagcgcgc
     3361 cgagcatcgg cggatcgggg aagcgatcgc cgagcagctc atcatcgacg agctcgcgcg
     3421 ctttcacccg ctcgcccgcc gccagccgat ccgccgtcac cagcatcggt cccagctcgc
     3481 gtgcgagctg atagacctgc tgaggattct tgccgcgctt cagcttcggc agtcgtggcg
     3541 cgagccagcg cgaccacgcc ggccgcgttg catcgtcgac gattgcatcg atttccttcg
     3601 cgagcgcgat gccagcgatc tgggtgtcga gatcaccggc aaacagcgtg cggagctcgc
     3661 cgagcgcgtc cttcgccgag agctcgccgc gccggaacgt ggcagcgacg tcgtcgccga
     3721 cgatcacccg tgcattcggc gacagacgcg cgagccactg cgtcgccggg cgatcctgcc
     3781 agtgtacggt gtagtagctg ctcgcggcgt tgccatcgac ccacgccgga cacgcggcgc
     3841 cgatcggtag ctcggtcttg tcgccgacga gcgtgcactg cggcgtcttc gtggcggggt
     3901 gcgcgatgca taccggcacg agccgcttgt cgcggg
//
LOCUS       DASUFX010000622         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_934251,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000622 DASUFX010000000
VERSION     DASUFX010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934251"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VFV99_25035"
     CDS             <1..1575
                     /locus_tag="VFV99_25035"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5062671.1"
                     /translation="AGLNPYANDFRPEVTNSEFHERYGHMPADQLEKITTVTTLAGRI
                     VLMRQMGKATFVTLRDASGADLQAFFKSDRLGAKAYEQIKLFDLGDIIGVAGTPMRTK
                     TGELTIAADGVRVLTKALRPLPDKGGHGFRDVEQRYRQRYVDLAVSPDVREVFRKRSA
                     IVRGIRRFLDARQFLEVETPMMHPIIGGAAARPFITHHNTLDMKLYMRIAPELYLKRL
                     VVGGFERVYEINRNFRNEGLSRQHNPEFTMLEFYQAYATYTDLMDLTEQMVGELALEV
                     NGSTKTTWDGIEVELAAPWRRLSIQDAVRDLGNVAESARVFADPAFAAETAIGLGISP
                     GEVARALLEGVPPGALGMPADEIVAGFKDPGHRPTLAAQMIERYPNEVEARVRAGHIG
                     YLVFEATAEAKLVQPTFLTQFPLAVSPLARKNDKDPAFCDRFELFVCGKEIANGFSEL
                     NDPDDQRSRFLAQLVAKATGAAETMDYDEDYCRALEIGMPPAAGEGIGIDRLTMMLTG
                     QASIRDVILFPLMRPE"
     gene            1575..>2336
                     /locus_tag="VFV99_25040"
     CDS             1575..>2336
                     /locus_tag="VFV99_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062672.1"
                     /translation="MPNVEADADRIRKEVQRRRTRGWAEVIIGAALIVVGLLITKLAH
                     DLVLYLAVSAPIIVGFITLLLGGLRLLRGDRDLAKPTQQPGQMHGSLNLGFGFKSFVA
                     WRYLMARDHRFSRIVLTVLVGAAFIFTIAYTAQSTLITVQNPLHLDHDGMLTLATAIL
                     IAKVAGSVLIYFALLIGALRYVFSFFTTVPLVGVWIGTGALVCVLAVMSGFETDLRDK
                     ILGSNAHIRVTREEGMMPEWAEVMKRIAKIPHVEAV"
BASE COUNT          398 a          786 c          742 g          410 t
ORIGIN      
        1 gccggcctga atccatacgc gaacgacttc cgccccgagg tcacgaacag cgagttccac
       61 gagcgctacg gccacatgcc ggccgatcag ctcgagaaga tcacgacggt gacgacgctc
      121 gccggccgca tcgtgttgat gcgccagatg ggcaaggcca ccttcgtgac gctgcgcgac
      181 gccagcggcg ccgatctgca agcgttcttc aagagcgatc gcctcggcgc gaaggcgtac
      241 gagcagatca agctcttcga cctcggcgac atcatcggcg tcgcgggcac gccgatgcgc
      301 acgaagacgg gcgagctcac catcgccgcc gacggcgtgc gcgtgttgac gaaggcgctg
      361 cggccgctgc ccgacaaggg cggccacggc ttccgcgacg tcgagcagcg ctatcgccag
      421 cgctacgtcg atctcgcggt gtcgcccgac gtccgcgagg tgttccgcaa gcgctcggcg
      481 atcgtacgcg gtatccgccg gttcctcgat gcacgccagt tcctcgaggt cgagacgccg
      541 atgatgcacc cgatcatcgg tggtgccgcg gcgcgtccgt tcatcacgca ccacaacacg
      601 ctcgacatga agctctacat gcgcatcgcg ccggagctgt acctgaagcg gctcgttgtc
      661 ggtggattcg agcgcgttta cgagatcaat cgcaacttcc gcaacgaagg gttgtcgcgt
      721 cagcacaacc ccgagttcac gatgctcgag ttctaccagg cgtacgctac gtacaccgac
      781 ctcatggacc tcacggaaca gatggtcggc gagctcgcgc tcgaggtgaa tggctcgacg
      841 aagacgacgt gggatggcat cgaggtcgag ctcgccgcgc cgtggcgccg gctgtcgatc
      901 caggacgccg tacgtgacct cggcaacgtt gccgaatcgg cacgcgtgtt tgcggatcct
      961 gcatttgctg cggagaccgc gatcggtctc ggcatttcac cgggcgaagt cgctcgtgcg
     1021 ctgctcgagg gcgtaccacc gggcgcgctt ggcatgcccg ccgacgaaat cgttgccggc
     1081 ttcaaggatc ccggccatcg accaacgctg gcagcgcaga tgatcgagcg ctatcccaat
     1141 gaggtggaag cccgcgtccg cgccggtcat atcggatacc tcgtgttcga ggcgaccgcc
     1201 gaggccaagc tggtccagcc gacgttcctc acccagttcc cgctcgcggt gtcgccgctc
     1261 gcgcgcaaga acgacaagga tccggcgttc tgcgatcgct tcgagctgtt cgtgtgcggc
     1321 aaggagatcg cgaacggctt ctccgagctc aacgatcccg acgaccagcg ctcgcgcttc
     1381 cttgctcagc tcgtcgccaa ggcgaccggc gcggccgaga cgatggatta cgacgaggat
     1441 tactgccgcg cactcgagat cggtatgccg cccgccgctg gtgagggcat cggcatcgat
     1501 cgccttacga tgatgctcac cggccaggca tcgattcgcg acgtgatcct gttcccactg
     1561 atgcgaccgg agtaatgccg aacgtggaag cagacgccga cagaattcgc aaagaggtcc
     1621 agcgccggcg cacgcgcggc tgggccgagg tgatcatcgg cgccgcgctg atcgtcgtcg
     1681 gtctcttgat cacgaagctc gcgcacgatc tggtgctgta tctcgcggtg tcggcgccga
     1741 tcatcgtcgg cttcatcacg ctgttgctcg gcggcctgcg cttgcttcgc ggcgaccgcg
     1801 acctcgcaaa gccgacgcag caaccgggcc agatgcacgg cagcctgaac ctcgggttcg
     1861 gcttcaagtc gttcgtcgcg tggcgctacc tgatggcgcg cgaccaccgc ttctcccgga
     1921 tcgtgctcac cgtgctcgtc ggtgcagcgt tcatcttcac catcgcttac accgcgcagt
     1981 cgacgctgat cacggtgcag aacccgctcc acctcgatca cgacggcatg ttgacgctcg
     2041 cgaccgcgat cctgatcgcg aaggtcgccg gcagcgtcct catctacttc gcgctgctga
     2101 tcggcgcgct ccgctacgtg ttctcgttct tcacgaccgt gccgctcgtc ggtgtctgga
     2161 tcggtacggg cgcgctcgtc tgcgtgctcg ccgtgatgag cggcttcgag accgacctgc
     2221 gcgacaagat cctcggctcc aacgcccaca tccgcgtcac ccgcgaggag ggcatgatgc
     2281 cggagtgggc ggaggtgatg aagcggatcg cgaagatccc gcacgtcgag gcggtg
//
LOCUS       DASUFX010000623         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_239996,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000623 DASUFX010000000
VERSION     DASUFX010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239996"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFV99_25045"
     CDS             complement(<1..970)
                     /locus_tag="VFV99_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062673.1"
                     /translation="MRSLAVLALAIACGPRSRPAAPAADIAARFPLAKLDDTALCDAL
                     LARRAEDHAIITDAEPRARRKVVVSDLHLGPGTADPKLAFIEDFYADAEWRPFLEREG
                     AKGPTDLIINGDFIEFWQLATAFGALPKLADPKQPPKGAVLGADQAFAVRAVERVIAA
                     HPDVFRGLGHFLDSGDHRVIIIPGNHDADLLWPKVQLAVARAINPRDPSRLMFVDGAA
                     YEHAGTYIAHGHAFDAANRFATQYAPFGRDRDGHCRLQTNWGEVFVDVFYTETERQIP
                     FIDNLSPEKAGVLWALRDNPDLARDLGAALRAVDMLRAAQGKGLNRD"
     gene            complement(1023..1433)
                     /locus_tag="VFV99_25050"
     CDS             complement(1023..1433)
                     /locus_tag="VFV99_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062674.1"
                     /translation="MRIVYVTTHVDFAQDIAEREVEQEIAGCLWDLAGVDAEAIEIVS
                     PLPLDAYERRALIRRLAAALPTRGGREVAYVASYLAITSDHEFAPVEAQWLDELQHGL
                     GITDERAADLAAAGAESATPGLRHLGEEELRTHH"
     gene            1575..2642
                     /locus_tag="VFV99_25055"
     CDS             1575..2642
                     /locus_tag="VFV99_25055"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5062675.1"
                     /translation="MRRRFLIALAIYSLAHLYIWWRLVLPLPSPAWQIATGIVALFAP
                     SFPINMRIRRRLMREMARPWLLVSFIWFGCIIYLLLGALASHLAVAFGANAREAAEIG
                     AATSLAVVAAGLLNVARGPVIKRVRVPLARLPVVSYTIAHLTDVHVGPLIGRPFIQRL
                     VERVNSLQPDLIVITGDLIDGPLSELRDHIEPMRGLRARDGVFAVTGNHEYYWNVEPW
                     LQHLRTLGMRVLRNEHVTIDGAFQLAGVDDASFGEDVARAVESRDPEKPLVLLAHHPR
                     TVARAKDAGVDLQLSGHTHGGQLLPWGWLARIWDPRVAGLARFGRTWLYVSHGTGFWG
                     PPMRVGTRCEIAAITLVPASD"
     gene            2699..3790
                     /locus_tag="VFV99_25060"
     CDS             2699..3790
                     /locus_tag="VFV99_25060"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEX5062676.1"
                     /translation="MKQMVCLVVLLGAAACGGDSGLGGGPPTLGTTPKEWEYVAIPGA
                     QCMNGTPTGIGVNLGTSGEVVMYMEGGGACFNDGTCGNVAHPSGWGPDQFGFNIGPYN
                     VGIFDRLDDKNPFRDATFVFVPYCTGDVHAGSKPDGIDGRKFVGYANVGLYLDYLVPK
                     SQDVRRVILAGSSAGGFGALMNFDRTQTAWGDTPVYLLDDSGPPLADMFLSPCLQQMF
                     RDAWNLDAALPASCTACRQADGGGLLNALGYLADAHPDSRMAIVTSTRDGVIRSFYGY
                     GYPDCVQGAQGLPMPEDVYASGIADLRDRAVASTSNFRVYSKDSGQHVWLLFGVDTVS
                     PRSDGTGEHLSQWLHDMLDPDSDWKSVAP"
     gene            complement(3787..4848)
                     /locus_tag="VFV99_25065"
     CDS             complement(3787..4848)
                     /locus_tag="VFV99_25065"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HEX5062677.1"
                     /translation="MRLHALIVTLAVGCSSAPELATGTQAIRAGTPAPDDNAVVAIGP
                     APVGCTEQLDARCTGTLIAPNAVLTAAHCARIARRIAVFVGADVARTGELHDVALSIV
                     HPSYQEGVATDDIAVLVLATAPSIAPLPLATTQTAGSDIGRTVRAVGYGGTDETGNDI
                     GVRRQGTSGIAAVNGATFRTTPMPSITCSGDSGGPVLIDRGSGEEIAGVTSQGDLRCE
                     TYTDNVDVVAHRAFIADAVATAMAWTPNGGAAASCKPGCVTDDDCALGLECDFLRATD
                     TTGVCALAPLEPGTLDGDCMRDDQCSSGTCARAPGEQACRCHVPCNTDDGGCNAQRRT
                     PGLAAFVLLVLLAIRARRR"
BASE COUNT          793 a         1631 c         1685 g          874 t
ORIGIN      
        1 cgtcgcggtt gaggcccttg ccttgtgccg cacgcagcat atcgacagca cgcagcgcgg
       61 cgccgaggtc gcgcgcgaga tcgggattgt cgcgcagcgc ccacagcacg cccgctttct
      121 cgggcgaaag attgtcgatg aacggaatct gacgctcggt ctccgtgtag aagacgtcga
      181 cgaagacctc tccccagttc gtttgcagcc gacagtgtcc gtcgcgatcg cgaccaaacg
      241 gcgcgtactg ggtggcgaac cgattcgcag catcgaacgc gtggccgtgc gcgatgtacg
      301 tccccgcgtg ctcgtaggcg gcgccgtcga caaacatgag ccgcgacgga tcgcgcggat
      361 tgatcgcgcg ggcgactgcg agctgcacct tcggccacaa cagatcggcg tcgtggttac
      421 ccggaatgat gatgacgcga tggtcaccgc tgtcgaggaa gtgaccgagg ccgcggaaga
      481 cgtcagggtg cgcggcgatc acgcgctcga cagcgcgcac ggcgaacgct tgatctgcgc
      541 cgagcaccgc gcccttcggc ggctgcttcg gatcggcaag ctttggcagc gcgccgaacg
      601 cggtcgcgag ctgccagaac tcgatgaagt caccattgat gatgaggtcg gtcgggccct
      661 tcgcgccttc gcgctcgagg aacggtcgcc actcggcatc ggcgtagaag tcttcgatga
      721 acgcgagctt cggatcggcg gtgccgggtc cgaggtgcag gtcgctgacg acgaccttgc
      781 ggcgcgcgcg tggctcggcg tccgtgatga tcgcgtggtc ctcagcgcgg cgggcgagca
      841 gcgcatcgca gagtgccgtg tcgtcgagct tcgcgagcgg aaagcgtgcc gcgatatccg
      901 ccgccggcgc ggccgggcgc gatcgcggac cacacgcgat agcgagcgcg agcacggcga
      961 gcgagcgcaa cgacgcaagt atgccactcg tccggtggcg cattccggca agttgtaacc
     1021 tgctagtggt gcgtgcgcag ctcttcttcg cccaggtgcc gcagtccggg cgtcgccgac
     1081 tccgcaccgg cggccgctag atcggcggcg cgttcatcgg tgatgccgag tccgtgctgg
     1141 agctcgtcga gccactgggc ctcgacgggc gcgaactcgt gatcggatgt gatcgcgaga
     1201 tacgacgcga cgtaagcgac ctcgcgtcca cctcgcgtcg gcagcgcagc ggcgagccgg
     1261 cgaatcaggg cgcggcgctc gtatgcgtcg agcggcagag gagagacgat ttcgatcgcc
     1321 tccgcgtcga cgccggcgag gtcccacagg caaccggcga tctcttgctc cacttcgcgc
     1381 tcggcgatgt cttgcgcgaa gtcgacatgg gtcgtcacgt agacaatgcg catgatcgtc
     1441 cgcacatcct cgggcagcag acgatcgcta ccgagaagct ctcgtaggaa gttgcggtcg
     1501 acgaagaggt gcatgagcga tgtaggagca agtgacgtgc cgacgcggcg cttccgccgt
     1561 gctagatccc ctctgtgcgc cgacgtttcc tgatcgcgct tgcgatctac tcgctagcgc
     1621 acctctacat ctggtggcgt ctcgtactgc cgttgccgtc tcctgcatgg cagatcgcga
     1681 ccggaatcgt ggccctgttc gcgccctcgt ttccgatcaa catgcggatc cgccggcggc
     1741 tgatgcgcga gatggcgcgg ccgtggctgc tcgtcagttt catctggttt ggctgcatca
     1801 tctacttgtt gctcggcgcg ctggcgagcc atctcgccgt cgcgttcggc gcgaacgcac
     1861 gcgaagccgc cgaaatcggt gcagcaacat cactcgccgt cgtggctgcg ggacttctca
     1921 acgttgcgcg tggcccggtg atcaagcggg tccgcgtgcc actcgcgcgg ctgccagtcg
     1981 tgtcgtacac gatcgcgcac cttaccgacg tgcacgtcgg cccgttgatc gggcgcccgt
     2041 tcatccagcg gctggtcgag cgcgtcaatt cattgcagcc cgatctgatc gtgatcaccg
     2101 gtgacctcat cgatgggccg ttgtccgagc tgcgcgatca catagagccg atgcgcgggc
     2161 ttcgcgctcg cgacggcgtg ttcgcggtca ccggcaacca cgagtactac tggaacgtcg
     2221 agccatggct ccagcatctc cggacgctcg ggatgcgcgt gctgcgcaac gagcacgtga
     2281 cgatcgacgg cgcgttccag ctcgcaggtg tcgacgacgc cagcttcggc gaggatgtcg
     2341 cgcgggcggt cgagtctcgc gatcccgaga agccgctcgt gctgctcgcg catcacccac
     2401 gcacggtggc gcgtgcgaag gatgcgggcg tcgatctgca attgtcgggt cacacacacg
     2461 gcgggcagct attgccgtgg ggttggctcg cgcggatctg ggatccgcgg gtcgcggggc
     2521 tcgcgcggtt cgggcggacc tggctgtatg tgagccatgg cacgggtttc tggggaccgc
     2581 cgatgcgcgt cggtacgcgc tgcgagatcg cagcgatcac gctggttccc gcgagcgact
     2641 gacggctttc gctgttttct ccgccacgcg cccggcgcga cggatacagt gcgggcccat
     2701 gaagcagatg gtttgtcttg tcgtccttct cggagccgcc gcgtgcggag gcgatagcgg
     2761 gttgggcggc gggccgccga cgctcgggac gacgccgaag gaatgggagt acgtagcgat
     2821 tcctggcgcg cagtgcatga atggcacgcc gaccggcatc ggcgtcaacc tcggcacgag
     2881 cggcgaagtc gtcatgtaca tggaaggtgg cggcgcctgc ttcaacgacg gaacctgcgg
     2941 caacgtcgcg catccgtcgg ggtgggggcc cgatcagttc ggtttcaaca tcggcccgta
     3001 caacgtcggc atcttcgatc gcctcgacga caagaatccg ttccgcgacg cgacgttcgt
     3061 cttcgttccg tactgcactg gcgacgtcca cgctggctcg aagcccgacg gcatcgacgg
     3121 ccgcaagttc gtcggttacg caaacgttgg cctgtatctc gactacctgg ttccgaaatc
     3181 gcaggacgtg cgccgcgtga ttctcgctgg ctcgtcggcg ggtggtttcg gcgcgctcat
     3241 gaacttcgac cgcacgcaga ccgcgtgggg cgacacgccg gtctacctgc tcgacgactc
     3301 cggtccgccg ctcgccgaca tgttcctgtc gccgtgccta cagcagatgt ttcgtgatgc
     3361 atggaacctc gacgccgcac tgccggcaag ctgcaccgcg tgccgacaag cggatggcgg
     3421 aggcctcttg aacgcgctcg gttacctcgc cgacgcgcat cccgacagcc gcatggcgat
     3481 cgtcacgagc acgcgcgacg gtgtgattcg cagcttctac ggctacggct atcccgactg
     3541 tgtccaagga gcgcaaggcc tcccgatgcc ggaggacgtg tacgccagtg gcatcgccga
     3601 tctccgtgac cgcgcagtcg cgagcacgtc gaacttccgc gtctactcca aagatagcgg
     3661 ccagcacgtc tggctgctgt tcggcgtcga tacggtgtcg ccgcggtccg atggaacggg
     3721 cgagcaccta tcgcagtggc ttcacgacat gctcgatcca gattctgact ggaagagcgt
     3781 cgcgccctaa cgccggcgcg cgcgaatcgc gagcagcacg agcaagacga acgcggcgag
     3841 cccgggcgtc ctgcgctgcg cgttgcagcc accgtcgtcg gtattgcacg gaacgtggca
     3901 gcgacaggct tgctcaccgg gagcgcgcgc acacgtgccg gagctgcatt gatcatcgcg
     3961 catgcagtcg ccatcgagcg tacccggctc cagaggcgcg agtgcgcaca cacccgtggt
     4021 gtcggttgcg cgaagaaagt cgcactcgag gccgagcgcg cagtcgtcgt cggtcacgca
     4081 accgggtttg cagctcgccg cagcgccgcc gttcggcgtc cacgccatcg ccgtcgcgac
     4141 tgcatcggcg ataaaggcgc ggtgtgcgac gacatcgacg ttatccgtgt acgtctcgca
     4201 gcgcagatcg ccttgcgagg tcacgccggc gatttcctcg ccgctgccgc gatcgatcag
     4261 caccggacca ccgctgtcgc cgctgcaggt gatactcggc atcggtgtgg ttcgaaacgt
     4321 cgcgccgttc acggccgcga tgccgctcgt gccctgtcgg cgcacgccga tgtcattacc
     4381 agtctcgtcg gtgccgccgt agccgacggc gcggacggtt cggccgatgt cgctgcccgc
     4441 agtttgtgtc gtcgccaatg gcagtggcgc gatgctggga gctgtcgcga gcacgaggac
     4501 tgcaatgtca tcggtggcga cgccctcctg gtagctcgga tgaacgatcg agagtgcgac
     4561 gtcgtggagc tcgccggtgc gcgcgacatc tgcgccgacg aagacggcaa tgcggcgcgc
     4621 gatccgagca caatgtgccg cagtcagcac ggcgttcggc gcgatcaacg tgcccgtaca
     4681 gcgcgcgtcg agctgctccg tgcatcccac gggcgccggg ccgatcgcga cgactgcgtt
     4741 gtcgtcgggc gctggcgtgc ctgcgcgaat cgcctgcgtg ccggtggcga gctccggcgc
     4801 agagctacag ccaactgcaa gcgtgacgat cagcgcgtgg agccgcactc agatcgagac
     4861 tagcacccag tgaccttgtg agcgctccac ccgcagcatg acctcgcgcg cgcctttgcc
     4921 ctcgaagttc accatcgcgc tcacgatgcg agcattgcgg tcgcgaccgt cctcgacctc
     4981 gtt
//
LOCUS       DASUFX010000624         8089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1350220, whole genome shotgun sequence.
ACCESSION   DASUFX010000624 DASUFX010000000
VERSION     DASUFX010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350220"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFV99_25070"
     CDS             <1..567
                     /locus_tag="VFV99_25070"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062678.1"
                     /translation="LKPEQLLIDAYGNVKVSDFGFTRIVERDHAVIRQIYVGMGNVAY
                     MAPELYTDPTATSPQTDIYALGIIFYELLTRKLPGRRSPMPSQINSALPHGIDDIFDK
                     MTRDAREDRYNSIDEILADIDKLEGTEKIVGKQNVVLVQESPLEAVKFRPGAEEPVAG
                     TPVGEGGDDDDKKGGHRPYSFQQRRKKI"
     gene            complement(590..1546)
                     /locus_tag="VFV99_25075"
     CDS             complement(590..1546)
                     /locus_tag="VFV99_25075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5062679.1"
                     /translation="MFELVKNGVLIAIVAHGLIGISLVWDKVLLKSPGTRNLYSYTFW
                     LGAISVFGLLLIPFGYTSPPIALIGIAFVAGVLHLVAVFFYYTALKHGEASESLAVMG
                     GFSPVATAAIGYFLLAQQMSFGQLFGFTLMTSGGFVMFFSEKLSLRRLLPPMLLASGL
                     LGLVNVLEKLVYNRTDFVGGYVWFTIGTFVAAMGMLAVPSWRRETFSESGQAKPRNRF
                     WYFVNRFMSGVGSFLVFYAISLAHPAIVDSILGVRYAVIFIGAYLLTKLRPDWLKEEF
                     RGWQLVTKLAATGLVIAGLVLVGIHGGKGKGGDSPTAMITTP"
     gene            complement(1570..2748)
                     /locus_tag="VFV99_25080"
     CDS             complement(1570..2748)
                     /locus_tag="VFV99_25080"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5062680.1"
                     /translation="MKLAMPHTPLDRWALSPAVKHLNHGSYGGCLRETIDAADAWRDR
                     LEAAPMQFLVLHWQTELDRARDVLATFLRAPSERLAFMPNATTGIAIALASSRIAAGD
                     ELITTSHTYRAAKNQLDRIAAANGARVVVVPIALPFDPDAFVAAFTGALTPRTKLALL
                     DHITSPTALQLPVDPLVAALAARGIPIIIDGAHAPGQIGLDVGALFDAGVTWYAGNNH
                     KWVCAPKGTGFLAASAAAPTLTPVVTSHGATPEYGPTNRFHAELDWMGTHDPTPHLSV
                     PTAITTIAGLANGWAHVIARNHALVIEMRKRLIAALGASELCSDDVLGTMAAVPITLP
                     ANTKPLALEKQLLAEGWEIPIVDWRDGPLVRISAHLYNHADEADQLAAKLRSLGVSGR
                     "
     gene            complement(2763..3326)
                     /locus_tag="VFV99_25085"
     CDS             complement(2763..3326)
                     /locus_tag="VFV99_25085"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062681.1"
                     /translation="MSWVQIVHEVPGRTRLLAKALRKDTARCERLAETLAAVPGVYRV
                     EARPYTGSLLVQHARSTTAQTLVETAARTLEAPVVARGATPPTPETVPALSEVARKLA
                     RALQDIDRDVRRASEGSVDLGTLITLGLLGAGAVQVARTEQLPLPPWFNLAWWAFRTF
                     ITLEVEEIAAKDPEERQIADEIEKVLE"
     gene            complement(3323..3568)
                     /locus_tag="VFV99_25090"
     CDS             complement(3323..3568)
                     /locus_tag="VFV99_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062682.1"
                     /translation="MRFHLPSYLLGVATGASGAAIVPRLRPVVMEIASGCYRLVDAVT
                     FRAARARENFSDFLAEAHARARDGAQRRIGRRERAHA"
     gene            complement(3565..6474)
                     /locus_tag="VFV99_25095"
     CDS             complement(3565..6474)
                     /locus_tag="VFV99_25095"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5062683.1"
                     /translation="MRSLPQPAANNPQQQPPIRDVLILADQPEIAATLARHARCSHRV
                     RLEIPSLLGDRELAARIECSANAQPGVIRAEANPHSARVLIEYAADAPIVDELEHLAH
                     TTRHRTVKRAGLQLAVDWHAEDSDDVCRRLATSCRDGLSARAAHERLDRFGPNVIADE
                     EVKSRLALLASQLQNAPTAMLLGSTLISLLLGDVLEAGAIVTVIALNTTIGYRVERTS
                     ESLLESWRATELGTADVIRDGSITTVPAADLVPGDLLVVRAGDMLVADARVVDAERLT
                     VDEASLTGESEPVAKDAKAVARSTMLAERRSMLYRGTTIASGHARAVVVATGPATEIA
                     SVQRLTAQSRAPKGRLQARLNALGVRLSWAGLAAAGATALAGLAHRRNPLEVVRDTVA
                     LGVAAIPEGLPVTTTAALVRAMARLRSRGIVVRRLATAETLGAITVACTDKTGTLTEN
                     RMRLEWISLLDGSRIRRIASSTLVANHMAVGSITALLVACVLNSDIEYQRNHCGTLEL
                     DGSATERALVEAARKIGIDPSSVRTYWERVRLIERGDGKHYVVSEHARGVAFIKGAPE
                     QVVPLCAVDDEVARELLAENYALAARGLRVLAVGYQQITRDARCDAGSWQFLGFVAMR
                     DPLRRGSVEALHAAERAGIRTVVLTGDQRATARAIAEEAELRGDIVEGHELDALLTAP
                     DARDRLERLAVVARVTPAQKVTVVEALRESGHVVAMLGDGINDAPALRAADVGVAVGA
                     ESTDLARQTADIVLRHPDLRSVLEAIAEGRAVQDNLRRSIGFQAAGNLGEILLAFGAA
                     VLGQRLIGSLGLLWINLLTDTFPGLALALEPTRGRLLERAPLPPTMPILDRSDWRRIA
                     RDGALLAASSGAAAIAGGPLAAFAAIGATQFGYATARRSSDHARGVQFGALVGGSAAL
                     HLAAVASTPARSLLRISGAPAIGLASFALGFGVPLYLGWRRSADYKIVRVAKESS"
     gene            6494..6676
                     /locus_tag="VFV99_25100"
     CDS             6494..6676
                     /locus_tag="VFV99_25100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062684.1"
                     /translation="MGHAIDQRSGLLAQGLPIRTPTQKRVGGSAKQNASGELWPGEKA
                     DAAEADQRRDRMLARE"
     gene            complement(6795..7868)
                     /locus_tag="VFV99_25105"
     CDS             complement(6795..7868)
                     /locus_tag="VFV99_25105"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5062685.1"
                     /translation="MNGVSPENWIDIPGVGPLLGMAVGDALGTTYEFDNIEQPDYPTL
                     AIGPATDVVGGGPFDLKAGQVTDDTQMAICIARSLTEKRALDMLDIATRYVAWYQHAF
                     DVGNQTGAALRAIENGESVGSAGRKVWHHSGRRAAGNGSLMRCAPIGVFFADAVKQAR
                     LGDLVEASLTDSLITHADPRCAIACAAFNAAIGAGLTTPAPNPGAQLDAARAAIPIAA
                     DKLRELWDDDRDDLALVDAAVEDLQRDLEAAVADNPRVYRVDLDLRRMAGFVRVAFRL
                     AFWHLVHTPSWRDALVDVASRGGDADTNAAIVGALLGARDGVTAIPRAWIDRVLAATQ
                     PGPPEWADAHHPKHLLTLAAALR"
BASE COUNT         1404 a         2610 c         2789 g         1286 t
ORIGIN      
        1 ctcaagccgg agcagctgct catcgacgcc tacggcaacg tgaaggtgtc cgactttggc
       61 ttcacgcgga tcgtcgagcg cgaccacgcg gtgattcgcc agatctacgt cggcatgggc
      121 aacgtcgcgt acatggcgcc ggagctctac accgatccga cggcgaccag cccgcagacg
      181 gacatctacg cgctcggcat catcttctac gagctgctga cgcggaagct gccggggcgc
      241 cgctcgccga tgccgtcgca gatcaacagc gcgctgccgc atggcatcga cgacatcttc
      301 gacaagatga cgcgcgatgc gcgcgaggat cgttacaact cgatcgacga gatcctcgcc
      361 gatatcgaca agctcgaggg gaccgagaag atcgtcggca agcagaacgt tgttctcgtc
      421 caggagagcc cgctcgaggc tgtgaagttc cggccgggcg ccgaagagcc ggtcgccggt
      481 acgccggtcg gcgaaggcgg cgacgacgac gacaagaagg gcggccaccg cccgtactcg
      541 ttccagcagc ggcgcaagaa gatctaagtg gtagcgaagc cgccactcgt catggcgtcg
      601 tgatcatcgc ggttggacta tcgccgccct tgcccttgcc gccgtgaatg cccacgagca
      661 cgagcccggc gatgaccagg ccagtggccg ccagcttggt gacgagctgc caaccacgga
      721 actcctcttt cagccaatca ggccgcagtt tggtcaggag gtatgcgccg atgaaaatga
      781 ccgcgtaacg aacgcccaag atcgagtcga cgatagcggg atgagcaaga ctgatcgcgt
      841 agaagaccag gaacgagccc accccggaca tgaagcggtt cacgaaatac cagaagcggt
      901 tacgcggctt cgcttggccg gactcggaga aggtctcgcg acgccacgac ggtacagcga
      961 gcattcccat ggcagcgacg aacgtgccga tggtgaacca gacgtatcca ccaacgaagt
     1021 cggtccgatt gtaaacgagc ttctccagca cattgaccag cccgagcaag ccggaagcga
     1081 gcagcatcgg gggaaggagt ctccgcaaag acagcttctc ggaaaagaac atgacgaacc
     1141 cgccgctggt catcagcgtg aatccgaaga gctggccaaa ggacatctgt tgggcgagca
     1201 ggaagtagcc gattgcggcg gtggcaaccg gggagaaacc gcccatcacc gcaagcgact
     1261 cggaagcctc accatgcttg agagcggtgt aatagaagaa gacggcgacg aggtgcagaa
     1321 ccccggctac gaacgcgatg ccgatcaacg cgatgggagg cgacgtgtat ccgaacggga
     1381 tgagaagcaa accgaacacg ctgatcgcgc cgagccagaa cgtgtagctg tagagattgc
     1441 gggtgccggg acttttgagc agtaccttgt cccagacgag cgaaatgccg atcagtccgt
     1501 gagcgacaat cgcgatcagt acgccgttct tgacgagctc gaacaaaaca tcctttcaca
     1561 gacggctacc taacgaccag atacgccgag cgagcgcagc ttcgcggcga gctggtctgc
     1621 ttcgtcggcg tggttgtaga ggtgcgccga gatccgcacg agcgggccgt cgcgccagtc
     1681 gacgatcgga atctcccagc cctcggcgag cagctgcttc tcgagcgcga gtggcttggt
     1741 gtttgccggc agcgtgatcg gtacggcggc catcgtgccg agcacgtcgt cgctgcacag
     1801 ctcgcttgcg ccgagcgcgg cgatgaggcg cttccgcatt tcgatcacga gcgcgtggtt
     1861 gcgcgcgatc acgtgcgccc agccgttcgc gagcccagca atcgtcgtga tcgcggtcgg
     1921 cacgctcagg tgtggcgtcg gatcatgcgt acccatccag tcgagctcgg cgtggaagcg
     1981 attcgtcggt ccgtattcgg gagtcgcacc gtgcgacgtg acaacgggcg tcagtgtcgg
     2041 cgcggcggcg gacgcggcga gaaagcccgt gcctttcggc gcacacaccc acttgtgatt
     2101 gttaccggcg taccaggtga cgcccgcgtc gaagagcgcg ccgacgtcga gcccgatctg
     2161 gcccggcgcg tgcgcgccat cgatgatgat gggaatcccg cgcgcggcga gcgccgcgac
     2221 gagcgggtcg accggcagct ggagcgcggt cgggctcgtg atgtgatcga ggagcgcgag
     2281 cttcgtgcgc ggcgtgagcg cgcccgtgaa agcagcgacg aatgcatcgg gatcgaaggg
     2341 cagggcgatc ggtacgacga cgacgcgcgc accgttggcc gccgcaattc gatcgagctg
     2401 gttcttcgcc gcgcgatacg tgtgactcgt cgtgatcagc tcgtcgcccg ctgcgatgcg
     2461 cgacgacgcg agtgcgatcg cgattccggt cgtcgcgttc ggcatgaacg cgagccgctc
     2521 actgggcgca cgtaggaacg tggcgagcac atcgcgcgca cggtcgagct cggtctgcca
     2581 gtgcagcacg aggaattgca tcggcgcggc ttcgaggcga tcgcgccagg cgtcggcggc
     2641 gtcgatcgtt tcgcgcaaac agccgccgta cgagccgtgg ttcagatgct tcacggcggg
     2701 cgacagtgcc cagcggtcga gcggcgtgtg cggcatcgcg agcttcacag cgcgagcata
     2761 tgtcactcca ggaccttctc gatctcatcc gctatctgtc gctcctcggg atccttcgcg
     2821 gcgatttctt ccacctcgag tgtgatgaag gtccggaacg cccaccaggc gaggttgaac
     2881 cacggcggta gcggcagttg ctcggtcctc gcgacctgaa cggcaccagc accgagcagg
     2941 ccgagcgtga tgagcgtgcc gagatcgacg ctgccctcgc tcgcgcggcg gacgtcgcga
     3001 tcgatgtcct gaagcgcccg tgcgagcttg cgcgcgacct ccgagagcgc aggcacggtt
     3061 tcgggcgtgg gtggtgtcgc accacgagcc accactggtg cttcgagcgt acgcgcggcg
     3121 gtttcgacga gcgtttgcgc cgtcgtggag cgtgcgtgct gcacgagcag actgccggtg
     3181 tagggccgcg cttcgacgcg atagacgccg ggaacggccg cgagcgtctc ggccagccgc
     3241 tcgcaacgcg cagtgtcttt gcgaagcgct ttcgcgagca ggcgggttcg cccggggacc
     3301 tcgtggacga tctggaccca gctcatgcgt gcgctctctc ccgccggcca atgcggcgct
     3361 gcgcaccgtc acgggcgcgc gcatgtgctt cggccaagaa gtcagagaag ttttcgcggg
     3421 cgcgtgccgc gcggaacgtg acggcatcga cgagtcgata gcagccgctc gcgatctcca
     3481 tcaccacggg acgcagccga ggaacgatcg cggcaccgct cgcgccggtg gcaactccga
     3541 gcaagtacga cggaaggtgg aatctcatga gctctccttt gcgacgcgaa cgattttgta
     3601 gtcagcgctt cggcgccaac cgaggtacag cgggacgccg aagccaagtg cgaacgatgc
     3661 gagaccgatc gctggcgcgc cactgatccg cagcagcgag cgagcgggtg tgctcgcgac
     3721 tgccgcgagg tgaagcgcag ccgaaccacc gacgagggcg ccgaattgca caccgcgcgc
     3781 atggtcgctc gatcgtctcg cggtcgcgta gccgaactgc gtcgcgccga tcgctgcaaa
     3841 cgcggcaagc ggaccgcccg cgatcgcggc agctcccgat gaggccgcga gcagtgcacc
     3901 gtcacgcgcg atgcgtcgcc agtcggagcg atcgaggatc ggcatggtcg gcggcagtgg
     3961 cgcacgctcg agcaagcgac cgcgcgtcgg ttcgagcgcg agcgcaagtc cggggaacgt
     4021 gtcggtgagg agattgatcc agagtaggcc gagcgagccg atcaagcgct ggccgagtac
     4081 ggcggcgccg aacgcgagca ggatctcgcc gagattgccg gcggcttgaa agccgatcga
     4141 acggcgcagg ttatcctgca cggcgcgacc ttccgcgatc gcctcgagaa ccgagcgcag
     4201 atcggggtgc cggagcacga tgtctgcggt ctggcgcgca agatcagtcg actcggcgcc
     4261 gaccgcgacg ccgacatccg cggcccgcag cgccggcgca tcgttgatgc catcaccgag
     4321 catcgcgacg acgtggccac tctcgcggag tgcttcgacg accgtgacct tctgcgccgg
     4381 ggtgacgcgt gcgacgacag cgagccgctc gagccggtcg cgcgcgtctg gagcagtgag
     4441 caacgcgtcg agctcgtggc cttcgacgat gtcgccgcgg agctctgctt cttcggcgat
     4501 cgcgcgtgcg gtcgcgcgct gatcgcccgt caggacgacc gtgcgaatgc cggcgcgttc
     4561 ggcggcatgg agtgcctcga ccgagccgcg gcggagtggg tcgcgcatcg cgacgaaacc
     4621 gaggaactgc cacgagcccg cgtcgcagcg cgcgtctcgc gttatctgct gataaccgac
     4681 ggcgagcacg cggaggcctc gcgctgccag cgcgtagttc tcggcgagca gctcgcgcgc
     4741 cacctcgtcg tcgacggcgc acaacggcac gacctgctcg ggtgcgccct tgatgaacgc
     4801 gacaccacgg gcgtgctcgc tgacgacgta gtgcttgccg tcgccgcgct cgatcaggcg
     4861 aacgcgctcc cagtacgtac gcaccgacga cggatcgatg ccgatcttgc gggcggcttc
     4921 gacgagcgcg cgctcggtcg cactgccgtc gagctcgagc gtgccgcagt ggttgcgttg
     4981 atactcgatg tcactgttca gcacgcacgc gacgagcagc gctgtgatcg agccgaccgc
     5041 catgtggttc gcgactagtg tcgagctcgc gatccggcgg atgcgcgatc cgtcgagcag
     5101 cgagatccac tcgagccgca tccggttctc ggtcagcgtc ccggtcttgt cagtacacgc
     5161 aacggtgatc gcaccgagtg tttcggcagt tgccagccgg cggacgacga tgccgcgtga
     5221 acgcagccgc gccatcgcgc gcacgagcgc ggccgtcgtg gtcaccggga ggccttctgg
     5281 aatcgccgcg acgccgagag cgaccgtgtc gcgcacgacc tcgagcgggt tacgccggtg
     5341 cgcaagcccg gcgagagcgg tggcgcctgc agccgcgagg ccggcccacg acagacgcac
     5401 gcccaaggcg ttgaggcgcg cctggagtcg tcccttcggc gcgcgcgatt gggcggtgag
     5461 tcgttgcacg ctcgcgatct cggtcgctgg cccggtcgcg acgacgactg cgcgcgcgtg
     5521 gcccgacgcg atcgtggtgc cgcgatagag catcgagcgg cgttcggcca gcatcgtgga
     5581 gcgggcgacg gccttggcgt ccttcgcaac gggctcgctc tcgccggtga gcgatgcttc
     5641 gtcgacggta agccgctcgg cgtcgacgac gcgtgcgtcc gcgaccagca tgtcgcccgc
     5701 gcgcacgacg agcagatcac ctggcaccag atcggctgcc ggtaccgtag tgatggagcc
     5761 atcgcgaatc acatcggcgg tgccgagctc ggtcgcacgc cacgattcga gcaagctttc
     5821 gctcgtgcgt tcgacgcggt atccgatcgt cgtgttgagt gcgatcaccg tgacgatcgc
     5881 gccagcttcg agcacgtcgc cgagcagaag cgagatcagc gtcgagccga gcagcatcgc
     5941 agtgggcgcg ttctggagct gcgacgcgag tagcgcgagg cgtgacttca cttcctcgtc
     6001 ggcaatgacg ttggggccga aacggtccag gcgttcgtgt gcagcgcgcg cagagagtcc
     6061 atcgcggcag ctcgtcgcga ggcgacggca tacgtcgtcg ctatcctcgg cgtgccagtc
     6121 gactgcgagc tgcagcccag ctcgcttcac ggtgcggtgc cgcgtggtat gtgccaggtg
     6181 ttcgagctcg tcgacgatcg gcgcatcggc cgcgtactcg atcaggacgc gcgccgaatg
     6241 cgggttcgct tccgcgcgga tgacaccggg ctgcgcgttt gcggaacact cgatgcgagc
     6301 ggcaagctcg cgatcgccga gcagactcgg aatctcgagg cgtacccggt gcgaacatcg
     6361 cgcgtggcgc gcgagtgtcg ccgcaatttc cggctggtcc gcgaggatca gcacgtcacg
     6421 gattggtggt tgctgctgcg ggttgttcgc cgcgggctgc ggcaacgatc gcacgcgctc
     6481 gctcgcgcac cgtatggggc acgcgatcga tcagcgctcg ggacttctcg cgcaaggact
     6541 cccgatccga acgccgacgc agaagcgtgt cggcggcagc gccaagcaga acgccagcgg
     6601 cgagctctgg cccggcgaga aggccgatgc cgccgaggcc gatcagcgtc gcgatcggat
     6661 gcttgcgcgc gaatgagccg agctttcccg tgctcgcgct cgtagcagtt gtttgttctt
     6721 cctgcatgac gagcaagcgg tgcatcgcac gtacccgcgc gctcgcgact tcgtcgtagc
     6781 tccgattcct gcggctagcg aagcgcggcc gcgagcgtca gcaaatgttt cggatgatgc
     6841 gcgtcggccc attccggagg accaggctgc gtcgccgcca gtacgcgatc gatccacgca
     6901 cgcgggatcg cggtgacgcc gtcgcgcgcg ccgagcagcg caccgacgat cgccgcgttc
     6961 gtatccgcgt cgccgccgcg gctcgcgaca tcgacgagcg catcgcgcca gctcggcgta
     7021 tggacgagat gccagaacgc gagccggaac gcgacgcgca cgaaacccgc catccgtcgt
     7081 aggtcgaggt cgacgcggta cacgcgtggg ttgtcggcaa ctgctgcctc gagatctcgc
     7141 tgcagatcct cgaccgcggc gtcgacgagc gcgaggtcat cgcgatcgtc gtcccacagc
     7201 tcgcgtagct tgtcggcggc gatcgggatc gcggcgcgtg cggcatctag ttgagcgccc
     7261 ggattcggtg cgggcgtcgt gagcccggcg ccgatcgccg cgttgaacgc cgcgcacgcg
     7321 atcgcacagc gcgggtcggc gtgcgtgatg agggagtcgg tgagcgacgc ctcgacgagg
     7381 tcaccgagcc gggcttgctt gacggcgtcc gcgaagaaca cgccgatcgg tgcgcagcgc
     7441 atcagcgagc cgttgccggc cgcgcgccgt ccgctgtggt gccacacctt gcggccggcg
     7501 ctgccgacgc tctcgccatt ctcgatcgcg cgcagtgccg ccccggtctg gttcccgaca
     7561 tcgaacgcgt gctgatacca ggcgacgtag cgggttgcga tgtcgagcat gtcgagcgcg
     7621 cgcttctcgg tcaggctccg cgcgatgcag atcgccatct gcgtgtcgtc ggtgacctga
     7681 cctgctttga ggtcaaacgg tccgccgccc acgacgtcgg tggcaggccc aatggcgagc
     7741 gtcgggtagt ccggttgttc gatgttatcg aactcgtagg tggtgccgag cgcgtcgccg
     7801 acggccatgc ccagaagtgg gccaacgccc ggaatatcga tccaattctc gggcgaaacg
     7861 ccgttcatgg tgttcatgtt acactatgaa agccggcggg caaggagatc ccgagatctc
     7921 tcccaagagg gcagttttct tggacgatcc agatccctcg gtgagattgg ccgggtgagc
     7981 gactctgccg cgcgtgcgcg cctcgaccgc tggaagcagt cccttctcga cccaaacgag
     8041 cgtctgttgg acctcgggga cgagggcatt ccaatcacca tggaccccg
//
LOCUS       DASUFX010000625         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_517925,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000625 DASUFX010000000
VERSION     DASUFX010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517925"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFV99_25110"
     CDS             <1..447
                     /locus_tag="VFV99_25110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5062686.1"
                     /translation="KGPPLSRDQVALAPIVKHVVDTLHARATEVGGTLTTEVADDVKL
                     RGDEDAIERVVLNLADNALRYGGKDVRVSIAARKEGDIVFVSVSDTGPGIPEEHRAKI
                     FDRFHRGNTTQEGSGLGLAIARELAIAMGGTLTLSGASRFTLELPA"
     gene            444..1175
                     /gene="modA"
                     /locus_tag="VFV99_25115"
     CDS             444..1175
                     /gene="modA"
                     /locus_tag="VFV99_25115"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5062687.1"
                     /translation="MTRVLLALMVVACHHEPPHVSVGVAASLRYAMPDVVAAYEKKSG
                     VTIDVTYGASDAIGDQLAHGAALDALVVADAVALDRQIAARTIDESSRREVATNAIVL
                     IGPPGSTPTFATLATLHVGTKIAIGDPATVPVGRYAKQYLESLGEWSTLEPQLIYGGD
                     VAGVLALAKQGRARLAVVYRTDARAAAPLVVLDDPPDAPKASVVAGIATHSKHAAAAR
                     AFTEFLASPEGHEILARYGFGPPRS"
     gene            1255..1980
                     /locus_tag="VFV99_25120"
     CDS             1255..1980
                     /locus_tag="VFV99_25120"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5062688.1"
                     /translation="MPRPAEDQSPTQPAWLGLRFVPGTTRVQQVIDNSPAAGAKFEAG
                     DEIASLDGVAVHTSQEIVKTVTESRPGTNMTVALTRRGAPLTITVQLAKRPTEDKLIS
                     DTLLNKPAPAFTAAALDGKSLALADLRGHVVLIDFWATWCGPCETQIEHLKQWHSTYA
                     PRGLQIVALSDEESDVVRKYVAEEHLTYQVGLDQDDHIRGTYLVQAMPTTVVIDKAGV
                     VRYVSVGTVAPAEIEAVIKKLLK"
BASE COUNT          359 a          685 c          682 g          309 t
ORIGIN      
        1 aagggcccac cgctatcgcg cgaccaggtc gcgctcgcac cgatcgtcaa gcacgtcgtc
       61 gacaccctgc atgcgcgcgc aaccgaagtc ggcggcacgc tcacgaccga ggtcgccgac
      121 gacgtgaagc tacgcggcga cgaggacgcg atcgaacgcg ttgttctcaa tcttgcggac
      181 aacgcgcttc gctatggcgg gaaggacgtg cgcgtgtcga tcgcggcgcg caaggaaggc
      241 gacatcgtct tcgtcagcgt gagcgacacc ggccccggca tccccgaaga acatcgcgcg
      301 aagattttcg atcgcttcca tcgcggcaac acgacgcaag aaggcagcgg cctcggcctc
      361 gcgatcgcgc gcgagctagc catcgcgatg ggcggcacgc tgacgctcag cggtgcgtcg
      421 aggttcacgc tggagctacc ggcatgacgc gcgtcctact cgccctcatg gtggttgcgt
      481 gtcatcacga gccgcctcac gtcagcgtcg gcgtggcggc atcgctgcgc tatgcaatgc
      541 cggacgttgt cgcggcgtac gaaaagaagt cgggtgtgac gatcgacgtc acgtacggcg
      601 cctcggatgc catcggcgat cagctcgcgc atggtgccgc gctcgatgca ctcgtggtcg
      661 cagacgccgt cgcgctcgac aggcagatcg ccgcgcgcac gatcgacgag agtagtcggc
      721 gcgaggtcgc gacgaacgcg atcgttctga tcggcccgcc tggctcgacg ccgacgttcg
      781 ccacgctggc gacgctgcac gtaggtacga agatcgcgat cggtgatcca gcgacggtgc
      841 cggtcggacg ctatgcgaag cagtatctcg agtcgctcgg cgagtggagt acgctcgaac
      901 ctcagctgat ctatggcggc gacgtcgccg gtgtcctcgc cctcgcgaag cagggccgcg
      961 cacggcttgc ggtcgtgtac cgtaccgatg cgcgtgcggc agcgcctctc gtcgtcctcg
     1021 atgatccacc agatgcaccg aaggcgagcg tcgtggccgg gattgcgaca cactctaagc
     1081 atgcggcggc tgcacgcgcg tttaccgagt tcttggcctc gccagagggg cacgagatct
     1141 tggcgcgtta cgggttcggg ccgcctcgca gctagcgcgc tcgcgctcgc gctactcggc
     1201 tgtcaccacg cgcctgcgca acccgctgcg tcaccgcccg cgccgccacc ggcaatgccg
     1261 cgacccgcgg aggaccagtc gccgacgcag ccggcgtggc tcggcctccg gttcgttccg
     1321 ggcacgacac gcgtgcagca agtgatcgac aacagcccgg ctgccggcgc caagttcgaa
     1381 gccggcgacg agatcgcgtc gctcgatggc gtcgcggtgc acacgtcgca ggagatcgtg
     1441 aagacggtga ccgagtcgcg acccggcacg aacatgacgg tcgcgctcac gcgccgcggc
     1501 gcaccgctga cgatcacggt gcagctcgcg aagcgaccga ccgaggacaa gctgatcagc
     1561 gacacgctgc tgaacaagcc ggcgccggca ttcaccgcgg ctgcgctcga cggcaaatcg
     1621 ctcgcgctcg cggacctgcg cgggcacgtc gtcctcatcg atttctgggc gacgtggtgc
     1681 ggaccgtgcg agacgcagat cgagcatctc aagcagtggc acagcacgta cgccccgcgc
     1741 gggctccaga tcgtcgcgct gtccgacgaa gagtcggacg tcgtgcgcaa gtacgtcgcg
     1801 gaggaacacc tcacctatca ggtcgggctc gatcaggacg atcacattcg cggcacgtac
     1861 ctcgtgcaag cgatgccgac cacggtggtg atcgacaagg cgggcgtcgt gcgctacgtc
     1921 agcgtcggca ccgtagcgcc ggccgagatc gaagccgtga tcaagaagct gctgaagtga
     1981 tcacggccag ctgacgccgc gcagatcgac gagctccgat ttatcgccga tgacg
//
LOCUS       DASUFX010000626         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_517977,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000626 DASUFX010000000
VERSION     DASUFX010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517977"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VFV99_25125"
     CDS             complement(<1..1631)
                     /locus_tag="VFV99_25125"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5062689.1"
                     /translation="MSAVEQEVPGNVKVLKSVCRSCHGGCSTLMHVENGKLTKVEGDP
                     EGPLNHGYLCPIGAATTQLVYHPDRLKYPQRRIGPKGSGNWQRISWDEALDEITEKVL
                     KIRAESGPESIAMGTGTGRHHIRWVSRFGNALGTPNWCEPGFAQCFHPRVNTCIQTMG
                     DLPVCDFTGGTPPAMVMYWGHNPPHSGPDGETRFNVREALLHKPRMITIDPRETVVAK
                     KSELWLQIRPGTDDALGLAFLNVIIEEDLYDHEFCEKYTHGFKELAEHVHKYTPEWAA
                     PITWVPADKIRAAARLFATVKPAMLEWGCAIEHTPKCIQTIRALSMIPAITGNIDVPG
                     GWIFGMKGAGRFPSLIENLDPEVEKKRLGYDKYKMLSSSEGADLPACHIPSLIDAMYY
                     GEPYRVRAFLVFGNNTLTTYANTNLVYKALMKLDFMVCADLFMTPTAELADIVLPAAS
                     WPEINQICALPTIAGNVVMSNQQTVRIGECKSDEEIFVELARRMKLPVCTDAVEDVLD
                     QMLRNGKQTVDFKGLTEQGWIHVPQKYRKYETQGF"
     gene            complement(1793..3226)
                     /locus_tag="VFV99_25130"
     CDS             complement(1793..3226)
                     /locus_tag="VFV99_25130"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5062690.1"
                     /translation="MGQAVARNEPCVQVVDLGGRTAIPGLIDGHTHFVRTAQAPGPFI
                     EGLEAARSIAELKAALAAAAKKAEPGEWIVAIGGFTPRQFAEKRLPTREELTAAVADH
                     PVYMQVGYSTRGIVNDEGRRVLAAASIATSDAGDVAPDGAGLTHVLRSTNEARMRRRF
                     ADYMTYAMSLGLTTVVDHACCDFVGAHLVPEERPNLHIAEQLWRSGELPLRLRIQYDH
                     RDIRDQNDIHSATARIANTTVGLGDDMYKVVRFGEQVIRAGASDDEVYQVYQRLADAG
                     WPLSQHTIREDEIERYIKIMERVAAKTPLAELRWSLEHVFEITPAQIARLKAIGVGVR
                     VQDHDYIRNQYSSWNAGPPFRTLLASGIKMGAGTDSGVVGALNPWLALYYMVTGKDAG
                     GDVIIPNEQISRQDALRLYTAGNAWYNFEEDRLGSIEPGKLADLVVLDRPYLTIPDEQ
                     LKEMKPVLTLIGGRVVHARAPYERLAN"
BASE COUNT          594 a         1161 c         1132 g          658 t
ORIGIN      
        1 ttgaagcctt gcgtctcgta cttcctgtac ttctgcggga cgtggatcca gccttgctcg
       61 gtcaggccct tgaagtccac ggtctgcttg ccgtttcgaa gcatctgatc gagcacgtcc
      121 tcgacggcgt ccgtgcacac gggcagcttc atgcgccgcg cgagctcgac gaagatctcc
      181 tcgtcggact tgcactcgcc gatgcggacg gtctgctggt tgctcatgac gacgttgccc
      241 gcgatcgtcg gcagcgcgca gatctggttg atctcgggcc atgacgcggc cggcagcacg
      301 atgtccgcga gctcggccgt cggcgtcatg aacagatcgg cacagaccat gaagtcgagc
      361 ttcatgagcg ccttgtagac gaggttcgtg ttcgcatacg tcgtcaacgt gttgttgccg
      421 aacacgagga acgcgcgcac gcgatacggc tcgccgtagt acatcgcgtc gatcagcgac
      481 ggaatgtggc acgcgggcag atcggcgccc tcggaggagg acagcatctt gtacttgtcg
      541 tagccgagcc gcttcttctc gacctcgggg tcgagattct cgatgaggct cgggaagcgg
      601 ccggcaccct tcatgccgaa gatccagccg ccgggcacgt cgatgttgcc cgtgatggcc
      661 gggatcatcg acagcgcgcg gatcgtctga atgcacttcg gcgtgtgctc gatcgcacag
      721 ccccactcga gcatcgcggg cttcacggta gcgaagagac gcgcagccgc ccgaatcttg
      781 tcggcgggca cccaggtgat cggcgccgcc cactccggcg tgtatttgtg aacgtgctcg
      841 gcgagctcct tgaagccgtg cgtgtacttc tcgcagaact cgtgatcgta gaggtcttcc
      901 tcgatgatca cgttcaagaa cgcgagcccg agcgcgtcgt cggtacccgg gcgaatctgc
      961 agccagagct cggacttctt cgcgacgacc gtctcgcgcg ggtcgatggt gatcatgcgc
     1021 ggcttgtgca gcagcgcctc gcggacgttg aaacgcgtct cgccatcggg gcccgagtga
     1081 ggcggattgt ggccccagta catgaccatg gccggcggcg tgccgcccgt aaagtcgcag
     1141 acgggcaagt cgcccatcgt ttgaatacag gtgttcacgc gcgggtggaa gcactgcgcg
     1201 aaacccggct cacaccagtt cggcgtgccg agcgcgttac cgaagcgcga tacccagcgg
     1261 atgtgatggc ggcccgtccc agtgcccatc gcgatcgact cgggcccgct ctcggcgcga
     1321 atcttgagga ccttctcggt gatctcgtcg agcgcctcgt cccacgagat gcgctgccag
     1381 ttgccggaac ccttcggtcc gatgcgccgc tgcggatact tcagccgatc ggggtgatag
     1441 acgagctgcg tcgtcgccgc gccgatcgga cacagatagc cgtgattcaa ggggccttcg
     1501 gggtcgccct cgaccttcgt gagcttgccg ttctcgacgt gcatcagcgt gctgcagccg
     1561 ccgtgacaac tgcggcacac gcttttcaga accttgacgt tccccgggac ctcttgctct
     1621 acagcgctca cgttcgttcc cccgaacaca ccgtatgcct cgacccggtc gagcttagca
     1681 gaagctcgca gacgcggcga tttacgccaa gtttgttgcg aaaacggcag gttcgctggc
     1741 accgtcgcga ggcgctgacc cgcgcgcgac ataagacccg actcgccagc tgctagttgg
     1801 cgagccgttc gtacggcgcg cgagcgtgga cgacgcgccc gccgatcaag gtgagcacgg
     1861 gcttcatttc cttgagctgc tcgtcgggaa tcgtcaggta cggtcgatcg agaacgacga
     1921 gatccgcgag cttgcccggc tcgatcgatc ccaggcgatc ctcctcgaag ttgtaccacg
     1981 cattgccggc cgtgtagagc cgcaaggcat cctggcgcga gatctgctca ttcgggatga
     2041 tcacgtcgcc gccggcgtcc ttgcccgtga ccatgtaata gagggccaac cacgggttca
     2101 acgcgccgac gacgcccgag tccgtgccgg cgcccatctt gatgccgctt gcgagcaatg
     2161 tgcgaaacgg cggccccgcg ttccagctcg agtactgatt gcggatgtag tcgtgatcct
     2221 gcacgcgcac accgacgccg atcgccttga gccgcgcgat ctgcgccggc gtgatctcga
     2281 acacgtgctc gaggctccag cgcagctcgg ccagtggagt cttcgcggcg acgcgctcca
     2341 tgatcttgat gtagcgctcg atctcgtcct cgcggatcgt gtgctgcgac agcggccagc
     2401 cggcatcggc gagccgttga tagacctggt agacctcgtc gtcggacgcg cccgctcgaa
     2461 tgacttgctc gccgaatcgc acgaccttgt acatgtcgtc gccaagcccg accgtcgtgt
     2521 tcgcgatgcg cgcggtcgcc gaatggatgt cgttctggtc gcggatgtcg cggtggtcgt
     2581 actgaatgcg caagcgcagc ggcagctcgc ccgaccgcca cagctgctct gcaatatgca
     2641 ggttcgggcg ctcctccggc acgaggtgcg cgccgacgaa gtcgcaacac gcgtgatcga
     2701 cgacggtcgt gagaccgagc gacatcgcat acgtcatgta atcggcgaac cgtcgcctca
     2761 tgcgcgcctc gttcgtcgaa cgcaggacgt gagtgagccc cgcgccgtcg ggagcgacgt
     2821 cgcccgcgtc ggacgtcgcg atgctcgccg ccgcgagcac gcgccggcct tcgtcattga
     2881 cgatgccgcg tgtcgagtaa ccgacttgca tgtaaacggg gtgatcggcg accgcggccg
     2941 tgagctcctc gcgcgtcggc aggcgctttt cggcgaactg tcgcggcgtg aagccgccga
     3001 tcgcgacgat ccactcgccg ggctcggctt ttttcgccgc ggccgcgagc gctgctttta
     3061 gctcggcgat cgagcgcgcc gcttcgaggc cttcgatgaa cggacccggc gcctgcgccg
     3121 tgcgcacgaa gtgcgtgtgc ccgtcgatca aaccggggat cgcggtgcgg ccgccgaggt
     3181 cgacgacctg cacgcacggt tcgttgcgcg cgacggcctg tcccacggtc agaaatcgac
     3241 cgtcgcgtat tcggacggtg ctcacggttc ggtccgctgc atccatcgtg agaattgtgc
     3301 cgttcgtgag aacccagtcc cggccaccgg ggcaggagct cgccgcttga ccccaagccg
     3361 gcgacccaca accgagaagt gcgcaacacg tgacgactgc gcgccacgga aagtggcgca
     3421 acgaaaacgc tcgtgcccgt tcaagaagat gcatgcgtat ctcccgtttt tggcgtccgc
     3481 cttacgatct tcggtcgaaa ccggtaacct acggagcccg aaatacctcg cggaagcgcg
     3541 acaac
//
LOCUS       DASUFX010000627         8231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_795137,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000627 DASUFX010000000
VERSION     DASUFX010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795137"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFV99_25135"
     CDS             complement(<1..730)
                     /locus_tag="VFV99_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062691.1"
                     /translation="MLTHNYSYEGALEDSVKVNWQVNDLIGGDKRLDFSKPFLPESLA
                     QVNGFKTFSAREKLLLNQIRGNSYLHLFAFVEEYILPFVTDEARSAMLGGGSINEVRA
                     LMTFAEEEAKHIHLFQRFAEEFTKGFHTPCGVIGPAADVAKVVLSHSRLGVALLTLHL
                     EWMTQAHYIDSVKNDQELDQQFANLLKHHWMEEAQHAKLDTMLVDKLARAGGAAAIEK
                     AFADFAALGGAIDGLLVQQVALDLD"
     gene            complement(804..1427)
                     /locus_tag="VFV99_25140"
     CDS             complement(804..1427)
                     /locus_tag="VFV99_25140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5062692.1"
                     /translation="MKRLSLIAMVLAGCGGGGGGDDSQAQSDAATGDTNGSGSADAAI
                     DSPAGAAFAVTSPTVTEGAAMMAVNTCDDQDTSPQLDWTGNNMSAMSFAVVLTDKSNN
                     NLVHWVIYDIPGNLTGLPADVEKVYAPTDVTGAHQTTSYTAAIRGYAGPCPPQGGGAH
                     NYEFAVYGVDVATLPGTSMATSRAQAVPLITQHMTAKATVTGMYTRQ"
     gene            1513..1851
                     /locus_tag="VFV99_25145"
     CDS             1513..1851
                     /locus_tag="VFV99_25145"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062693.1"
                     /translation="MSNGVPVRLSEKLAVRARAVAEMQDRSLTEQVEHWALLGQVVEE
                     AIMSATVQRLKVRSHDEALGDRLAFAKTPEGQSKAAKLIHERNPIRHDINGTGTIRVL
                     RNGQKRTKKR"
     gene            1848..2429
                     /locus_tag="VFV99_25150"
     CDS             1848..2429
                     /locus_tag="VFV99_25150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062694.1"
                     /translation="MTPQLVLLAGPNGAGKSTFYDVFLANSPLPFLNADLFAAETGIE
                     SLEAARILDATRDRMIEDGLGFITETVFSDPGGHKLGMLKRAVDAGYDVTLIYIGVVS
                     ADLLPRRVDQRIAGGGHDVPRDRIVSRFERSLANLRTAIAFVPTVELYDNSSSETPYQ
                     HVATFKNGVLQERMSGRLPNWARGIVPSARSKK"
     gene            2620..3912
                     /locus_tag="VFV99_25155"
     CDS             2620..3912
                     /locus_tag="VFV99_25155"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062695.1"
                     /translation="MTRSLFACVGLSLLGSMIGCADSGSSEDCLPGDAECAAPLGDGK
                     ADGFDGKNDPEVMAQHLEYHLDQLPKKGWRDKPVWKDSYPEAVGRAETVWADTYWPAS
                     EGSHNARWQGASEKSPLEKYDAAFNNAPGCATQPEMYGAGAKAAWDTYNNCAGPAAKW
                     QTENYESIGLMHNGIDDDGDGRTDEYGSDGIDGVQGWWGSCHAWTPASQLIPEPQKAV
                     TINGVRFDVGDIKALIQNAFDYTEAVMLGGRCNAKTITHDVNGSANDECSDVNPGALH
                     VVMTNFLGINQLALVEDRTANYEVWNQPVVGYEITKQDEVTPTVAMQCVGGTGDTYTY
                     NTNAKKLYEVRMTVSYVGESGAGNTPIGYRNNVSTDDYHYILEIGSTGKVIGGRYCKD
                     GENRHIDFLWAPTGDWQPSNPHVDVAKVKQLIKASIAP"
     gene            3937..5295
                     /locus_tag="VFV99_25160"
     CDS             3937..5295
                     /locus_tag="VFV99_25160"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5062696.1"
                     /translation="MTRLALLAVVAACGSSPRETRPLSSDGTHLRDDSGRIALLRGVN
                     ARASGIFDVTFDDGRIPLEPIPALEAADCKRMRELGLDLLRLPINWSGIEPTRGSYDE
                     AYLQRVDAAVRCAGDAGVYVMIDLHQDAYSKEIGEDGAPLWAIEPPPTMLLQGPLTDL
                     GDRRTSAQVTAAFETFFDQDDPKGLQAAFFAMLDHVAARWADDPAVIGFELFNEPSVG
                     QVDVDPFNDKAAARVRAAAPDKLVFFEPSATRNLFDFAPKPPGAFSVENSVYAPHIYT
                     FVFYSDRTRLEQLVPADLEPSVAAAREEANVWQTPLVIGEYGIGPTDTNADLWMGVQA
                     ELHDRYLASDAFWVWKEDSQASWGVFDHTGGVWTERPQVVAWLSRVHAARIAGEVVAN
                     EYDHVTRTLHLETHSSAAHAIYVPAPGFTATCNGTQLSGERDPATGLVELACDGMLVV
                     AP"
     gene            5320..6093
                     /gene="nudC"
                     /locus_tag="VFV99_25165"
     CDS             5320..6093
                     /gene="nudC"
                     /locus_tag="VFV99_25165"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX5062697.1"
                     /translation="MAFRPGFASTAERGRIYAVAGQSLHVVREGGAARIPVGAVPAGA
                     LYMGELDGEPCFAVHGASELGEAVALRQLFGALSDEEFGIAGRALGLTAWDRDHRFCG
                     RCGAETERSAVERVRTCKQCNHGAYPRLSPAVIMLVERDGKALLARNARFPLPFFSTL
                     AGFVEVGETLEQAVAREVGEEAGVHIADIRYFGSQPWPFTGSLMIGFTAQWAGGEIEA
                     DPTELAEANWFAPDALPIVPPKLSIARELIDDFVRRAAK"
     gene            6169..6468
                     /locus_tag="VFV99_25170"
     CDS             6169..6468
                     /locus_tag="VFV99_25170"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5062698.1"
                     /translation="MAYQFAALADPTRRQVFERLAKRSLSVGELAAGLPVSRPAVSQH
                     LRVLEQARLVSSRRDGARRLYSVDPRGIEAMRDYLDKFWDRALAAFKEAAEKDDE"
     gene            6465..6905
                     /locus_tag="VFV99_25175"
     CDS             6465..6905
                     /locus_tag="VFV99_25175"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5062699.1"
                     /translation="MKITKQLTVEAPIEKAFRVFTANMSTWWPKQHHIGKSPLKTCVV
                     EPKVGGRWFEICEDDSQCDWGKVLAWDPPNRVMLAWQLNGQFAYDPSLITEVEVKFTA
                     LSATRTRVDFEHRDLDKIGTKGTVAEMDHGWGQILEAFSATAAA"
     gene            6981..7865
                     /locus_tag="VFV99_25180"
     CDS             6981..7865
                     /locus_tag="VFV99_25180"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5062700.1"
                     /translation="MRWIWCAVLASGCVSIDDTPKPWVAAGQIAAPLTPELGPAVTPP
                     RVPTTLRIASWNVERGEHPDALADEIMTSPVLASADILIVQEIENHPGELGSRASRLA
                     AALGMTWFYAPARTQDEGTHGDAILSRFPLSSPEVKQLPFVDQPVNSRTRIAQRAVVD
                     LGTRTFTLVNVHLDTRLAPVDRIRQLDPAVADNPPVTVVGGDLNTLPWIWVDTAVPLT
                     SSEAVLGQDQAQIVDDYLAAQGFATPIVHDTDTHESVIDMRLDALCPRGLPVLDAGVD
                     YTVSGSDHYAVWIDIDVR"
     gene            complement(7862..>8231)
                     /locus_tag="VFV99_25185"
     CDS             complement(7862..>8231)
                     /locus_tag="VFV99_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5062701.1"
                     /translation="YRLPKPQDGMPELPISLHVPSGGGGGVAVIVDKRVQLTTPQYEL
                     VNLLVQRMRSDAGKPPDTRGFVPVSALLRLSLDVADPGEQHVRQLVYRVRRLLLNAGI
                     GDLIEVRRGVGYRLRVVPRP"
BASE COUNT         1445 a         2641 c         2743 g         1402 t
ORIGIN      
        1 agtcgaggtc gagcgcgacc tgctggacca agagcccgtc gatcgcgccg ccgagcgcgg
       61 cgaagtccgc gaacgccttc tcgatcgccg cggcgccgcc cgcgcgcgcg agcttgtcga
      121 cgagcatcgt gtcgagcttc gcgtgctgcg cctcctccat ccagtgatgc ttgaggaggt
      181 tcgcgaactg ctgatcgagc tcttggtcgt tcttcacgct gtcgatgtag tgcgcctgcg
      241 tcatccactc gaggtggagc gtgagcagcg cgacgccgag ccgcgagtgc gagagcacga
      301 ccttcgcgac gtcagccgcc ggaccgatca cgccgcacgg ggtgtggaag cccttcgtga
      361 actcttccgc gaagcgctgg aacaggtgga tgtgctttgc ctcttcttcc gcgaacgtca
      421 tcaacgcgcg gacctcgttg atgctgccgc cgccgagcat cgcgctccgc gcttcatcgg
      481 tgacgaacgg gaggatgtac tcctcgacga acgcgaacag gtggaggtag ctgttgccgc
      541 ggatctggtt gagcagcagt ttttcgcgtg cactgaacgt cttgaagccg ttcacctgcg
      601 cgagcgactc cgggaggaac ggcttcgaaa agtcgaggcg cttatccccg ccaatcagat
      661 cgttcacctg ccagttcacc ttcaccgaat cttcgagtgc gccttcgtag ctgtagttgt
      721 gcgtgagcat gccgttcccc tttggtcgag agcacgctac cgcgaggact ggctcacgcc
      781 tagggctcgg gcgacctaga atgtcattgc cgggtgtaca tgccggtcac cgtcgccttc
      841 gccgtcatgt gctgcgtgat cagcgggacc gcctgcgcgc gactcgtcgc catcgacgtg
      901 ccgggcagcg tcgcaacatc gacgccgtag acggcgaact cgtagttgtg cgcgcctccg
      961 ccttgcggag ggcacggccc tgcgtagccg cgaatcgctg ccgtgtatga agtcgtctgg
     1021 tgcgcaccag tgacatcggt cggcgcgtac accttctcga cgtcagcggg taggccggtc
     1081 aggttccccg ggatgtcgta gatcacccag tgcacgagat tgttgttcga cttgtcggtg
     1141 agcacgaccg cgaagctcat cgcgctcatg ttgttgccgg tccagtcgag ctgtggcgac
     1201 gtgtcctgat cgtcgcaggt gttcaccgcc atcatcgcgg cgccctcggt gaccgtgggg
     1261 ctcgtcaccg cgaacgccgc gcccgcaggg ctgtcgatcg cggcgtccgc ggagccggat
     1321 ccattcgtgt cgccagtcgc tgcgtcggac tgcgcttgcg agtcgtcacc gccaccacca
     1381 ccaccacaac ccgcgagaac cattgcaatg agagataagc gcttcacgga acggagctta
     1441 ggcctgtcag cacccggctg accactaatt tggctttgtg gtgatgtagc agaatgctac
     1501 atttcgatca ccatgagtaa cggcgttccg gttcgcctga gcgagaagct tgccgtgcgt
     1561 gcacgcgctg tcgccgagat gcaggaccgc tcgctgacag agcaggtcga gcactgggcg
     1621 ctgctcggtc aggtcgtcga agaggcgatc atgtctgcaa cagtgcagcg tctcaaggta
     1681 cgcagccacg acgaggcgct cggcgatcgc ctcgcgtttg cgaagacgcc agagggccag
     1741 agcaaggcgg cgaagctgat ccacgagcgc aatccgatcc ggcacgacat caacggtacc
     1801 ggcacgattc gtgtgctgcg taacggtcaa aagcgcacga aaaagcgatg acaccacagc
     1861 tcgtcctgct tgcgggtccg aacggggccg gcaagtcgac gttctatgac gtcttcctcg
     1921 ccaattcccc gctgccgttt ctcaatgccg acctctttgc tgcggagacc ggtatcgagt
     1981 cgctcgaggc tgcgcgcatc ctcgatgcaa cgcgcgatcg catgatcgaa gatggtctcg
     2041 gtttcatcac cgagaccgtg ttctctgatc caggcgggca caagctcggg atgctaaagc
     2101 gcgccgtcga cgcgggctac gacgtgacac tgatctacat cggcgtagta agcgcagacc
     2161 tcttaccgcg ccgcgtggat cagcgcatcg cgggtggcgg tcacgacgtt ccccgggatc
     2221 gcatcgtgtc gcgtttcgag cggtcgctcg cgaacttgcg cactgcgatc gcgttcgtgc
     2281 cgaccgtcga gctctacgac aacagctcca gcgaaactcc ataccaacac gtcgcgacgt
     2341 tcaagaacgg cgttcttcaa gaacgcatgt cggggcggtt gcccaactgg gcgcgaggca
     2401 tcgttccgtc ggcgcgctcg aagaaatagg gttcgtgcac gcttctgcgc aacgcaacga
     2461 tggctcggcc gcgttctgcg ttggcagatg gatctgccgg caagcgcatt cgaattcagc
     2521 atctgcggcg gctgggggtg tagccggtga ctctcggtaa ttcgccgaaa tcgcgtctgc
     2581 gtccgagtgg cacgagcgat gccatggggg acgcgcatca tgacgcgctc tctcttcgca
     2641 tgtgttggtc tctcgctcct cggttcgatg atcggctgcg ccgactcggg ctcatctgaa
     2701 gattgcttgc ccggcgatgc cgaatgcgcc gcgccgctcg gcgacggcaa ggccgacggc
     2761 ttcgacggca agaacgatcc ggaagtgatg gcgcagcatc tcgagtacca cctcgatcag
     2821 ctgccgaaga aaggctggcg cgacaagccg gtgtggaagg acagctatcc cgaggcggtt
     2881 ggccgcgcgg agacggtgtg ggccgacacg tactggccag catcggaagg ctcgcacaac
     2941 gcacgctggc aaggcgcgag cgagaagtcg ccgctcgaga agtacgacgc ggcgttcaac
     3001 aacgcgcccg gttgcgcgac gcagccggag atgtacggcg cgggcgcgaa ggccgcttgg
     3061 gacacgtaca acaactgcgc gggtccggct gcgaagtggc agaccgagaa ctacgagagc
     3121 atcggcctca tgcacaacgg catcgacgac gatggcgacg gccgcaccga cgagtacggt
     3181 agcgacggca tcgatggcgt gcagggctgg tggggcagct gccatgcgtg gaccccggca
     3241 tcgcagctca ttcccgagcc gcagaaggca gtcacgatca atggcgtgcg cttcgacgtc
     3301 ggcgatatca aggcgctgat ccagaacgcg ttcgactaca ccgaggccgt catgctcggt
     3361 ggtcgctgca acgcgaagac gatcacgcac gacgtcaacg gctcggcgaa cgacgagtgc
     3421 tcggacgtca acccgggcgc gctgcacgtc gtgatgacga acttcctcgg catcaaccag
     3481 ctcgcgctgg tcgaggaccg cacggcgaac tacgaagtgt ggaaccagcc ggtcgtcggc
     3541 tacgagatca cgaagcagga cgaagtgacg cccacggtcg cgatgcagtg cgtcggcgga
     3601 accggtgaca cgtacacgta caacacgaac gcgaagaaac tgtacgaagt tcggatgacc
     3661 gtcagctacg tcggcgagag cggcgcgggc aacacgccga tcggctatcg caacaatgtc
     3721 tccaccgacg actaccacta catcctcgag atcggctcga ccggcaaagt gattggcggt
     3781 cggtattgca aggacggcga gaaccgtcac atcgacttcc tgtgggcgcc gacgggtgac
     3841 tggcagccga gcaacccgca cgtcgacgtc gcgaaggtca agcagctgat caaagcgtct
     3901 atcgcgccct gatccgactc gctaggatgc gggcgcatga cgcgcctcgc attgctcgca
     3961 gtggttgccg cttgcggcag ctcaccacgc gagactcggc cactctcatc cgacggtaca
     4021 caccttcgcg atgacagtgg ccgcattgcg cttctgcgcg gcgtgaacgc gcgtgcctcc
     4081 ggcatcttcg acgtcacgtt cgacgatggt cgcattccgc tcgagccgat tcccgcgctc
     4141 gaggcggccg actgcaagcg catgcgcgag ctcgggctcg atttgctgcg actgccgatc
     4201 aactggagcg gcatcgagcc gacgcgcggc agctatgacg aggcatatct ccagcgcgtc
     4261 gacgcggcgg tgcgctgcgc gggtgacgct ggggtgtacg tgatgatcga tctgcaccag
     4321 gacgcgtact cgaaggagat cggcgaagac ggcgcgccgc tgtgggcgat cgagccgccg
     4381 ccgacgatgc tgctgcaagg tccgctgacg gatctcggcg accgccgcac gtccgcgcag
     4441 gtcacggcgg cgttcgagac gttcttcgat caggacgatc cgaagggcct gcaggccgcg
     4501 ttcttcgcga tgctcgatca tgtcgccgcg cggtgggccg acgatccggc cgtgatcggg
     4561 ttcgagctgt tcaacgagcc gtcggtcggg caggtcgacg tcgatccgtt caacgacaag
     4621 gcggcggcgc gcgtgcgtgc ggccgcgccc gacaagctcg tgttcttcga gccgagcgcg
     4681 acgcgcaacc tgttcgactt cgcgccgaag ccgccgggcg cgttctccgt cgagaattcg
     4741 gtctacgcgc cgcacatcta cacgttcgtg ttctattccg atcggactcg actcgagcag
     4801 ctcgtacctg ccgatctcga gccgagcgtg gcggctgcgc gcgaggaggc caacgtgtgg
     4861 cagacgccgc tcgtgattgg cgagtacggc atcggaccga ccgacacgaa cgccgatctg
     4921 tggatgggcg tgcaagcgga gctgcacgat cgctatctcg cgagcgacgc gttctgggtc
     4981 tggaaggaag acagccaggc atcgtggggc gtgttcgatc acaccggtgg tgtgtggacc
     5041 gagcggccac aggtcgtcgc ctggctatcg cgcgtgcacg cggcgcggat cgccggcgag
     5101 gtcgttgcga acgaatacga tcacgtcacg cgcacgctgc acctcgagac gcactcgtcg
     5161 gcggcccacg cgatctacgt gccggcgccc ggcttcacgg cgacctgcaa cggcacccag
     5221 ctcagcggcg agcgcgatcc ggcaaccggg ctcgtcgagc tcgcgtgcga cggcatgctc
     5281 gtcgtcgcgc cgtagccgca agcgtggtac gccggggaca tggcatttcg tcccggcttc
     5341 gcgagcaccg ccgagcgtgg ccgcatctac gcggtcgccg gtcagtcgct gcacgtcgtg
     5401 cgcgagggcg gggcggcgcg gattccggtc ggtgcggtgc cagccggcgc gctgtacatg
     5461 ggcgagctcg atggcgagcc gtgcttcgcg gtgcatggcg cgagcgagct tggcgaagcg
     5521 gtcgcgctgc gccagctgtt cggcgcgctg tccgatgagg agttcggaat cgccggtcgc
     5581 gcgctcggcc tcaccgcgtg ggatcgcgat catcggttct gcggccgctg cggtgcggag
     5641 accgaacgca gcgcggtcga gcgcgtacgc acgtgcaagc agtgcaatca tggcgcgtat
     5701 ccgcggctgt cgccggccgt gatcatgctc gtcgagcgcg acggcaaagc gctgctggca
     5761 cgcaacgcgc gctttccgct accgttcttc agcacgctcg ccggtttcgt cgaggtcggt
     5821 gagacgctcg agcaagcggt cgcgcgcgag gtcggcgaag aagcgggcgt gcacattgcc
     5881 gacattcgct acttcggcag tcagccgtgg ccattcaccg gctcgctgat gatcggattc
     5941 actgcgcagt gggcaggcgg cgagatcgaa gccgatccga ccgagctcgc cgaggcgaac
     6001 tggttcgcgc cggatgcgct gccgatcgtg ccgccgaagc tatcgatcgc gcgcgagctg
     6061 atcgacgact tcgttcggcg cgctgcgaag taactcgcgg gcttatgaat gcggcggtcg
     6121 ttcgggcttg acttcctcgg agtggtaagt gaagacttac ggtaagtggt ggcttaccag
     6181 ttcgcagcac tcgcagatcc gacgcgccga caggtattcg aacgcctggc gaagcggtcg
     6241 ctctcggtcg gagagctcgc cgccggattg ccggtgtcgc ggccagcggt ctcgcagcac
     6301 ttgagggtgc tcgagcaggc gcgactcgtt agctcacgac gtgacggcgc acggcgcctc
     6361 tattccgtcg atccccgcgg catcgaggcc atgcgcgact acctcgacaa gttctgggat
     6421 cgagcactcg ccgcgttcaa agaagcggcc gaaaaggatg acgaatgaag atcacgaagc
     6481 agctcaccgt cgaagcaccg atcgagaagg cgttccgcgt gttcaccgcg aacatgtcga
     6541 cgtggtggcc gaagcagcac cacatcggca agagcccact caagacctgc gtcgtcgagc
     6601 cgaaggtcgg cggccgctgg ttcgaaatct gcgaggacga cagtcaatgt gactggggca
     6661 aggtgctcgc atgggatccg ccgaaccgcg tcatgctcgc gtggcagctg aacggccagt
     6721 tcgcgtacga cccgagcttg atcaccgagg tcgaggtgaa gttcaccgcg ttgtcggcga
     6781 cccgcacgcg cgtcgacttc gagcatcgcg acctcgacaa gatcggcacc aagggcacgg
     6841 tcgccgagat ggatcacggc tggggccaga tcctcgaggc gttcagcgcg actgcagctg
     6901 cgtagctggt gttctgcggc aaccggtggc acctgcatgc cgtcggccaa cgctgcgccg
     6961 cgcgtgggcg actatcaacc atgcgctgga tctggtgcgc ggtactcgcg agcggctgcg
     7021 tgtcgatcga cgacacgccc aagccgtggg tcgccgccgg tcagatcgcc gcaccgctga
     7081 cgcccgagct cgggcctgct gtgacgccac cacgcgtgcc gacgacgctg cgcatcgcaa
     7141 gctggaacgt cgagcgcggc gaacaccccg acgcgctcgc cgacgagatc atgacgtcgc
     7201 cagtgctcgc gtccgccgac atcttgatcg tccaggagat cgagaatcac cccggcgagc
     7261 tcggcagccg cgcatcgcgc ctcgctgcag cgctcggcat gacgtggttc tacgcgcccg
     7321 cgcgtactca agatgagggc acgcacggcg atgcgatcct cagtcgcttt ccgctgtcgt
     7381 cacccgaggt gaagcagctg ccgttcgtcg atcagccggt caacagccgc acgcgcatcg
     7441 cgcagcgcgc ggtggtcgat ctcggcacgc gcacgttcac gctcgtgaac gttcacctcg
     7501 acacgcgcct cgcgcccgtc gaccgcattc gccagctcga tccggcggtc gccgacaatc
     7561 cgccagtgac ggtggtcggc ggcgatctca acacgctgcc gtggatctgg gtcgacacgg
     7621 cagtgccgct cacgagcagc gaggccgtgc tcggccagga ccaggcacag atcgtcgacg
     7681 actaccttgc tgcgcaggga ttcgcgacgc cgatcgtgca tgacaccgac acgcacgaga
     7741 gcgtgatcga catgcgtctc gacgcgctat gtccgcgtgg attgccggtg ctcgatgccg
     7801 gtgtcgacta caccgtgagc ggcagcgacc actacgcggt gtggatcgac atcgacgtgc
     7861 gctaaggccg tggtacgacg cgcaagcgat agccgacgcc gcgccgcact tcgatcagat
     7921 cgccgatgcc agcgttgagc agcaagcgcc gcacgcgata gaccagctgc cggacgtgtt
     7981 gctcgccggg atcggcgacg tcgagtgaca gccggagcag cgcgctgacc gggacgaagc
     8041 cgcgcgtatc gggtggtttg ccggcatcgc tgcgcatgcg ctggacgagc agattgacca
     8101 gctcgtactg cggcgtggtc agctgcacgc gcttgtcgac gatcacggcg acgccgccac
     8161 cgccgccgct cggcacgtgc aatgaaatcg gcagctcggg catgccgtcc tgcggtttcg
     8221 gaaggcgata g
//
LOCUS       DASUFX010000628         6391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_240804,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000628 DASUFX010000000
VERSION     DASUFX010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240804"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..398
                     /locus_tag="VFV99_25190"
     CDS             144..398
                     /locus_tag="VFV99_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062702.1"
                     /translation="MNRLIDYLRQNILIDFQGDLSVAKVRELLAGDESRDAKALLTKL
                     VADRKVEDMLVVLADCLTEPVQKALTDDVMREQLRMYSES"
     gene            441..719
                     /locus_tag="VFV99_25195"
     CDS             441..719
                     /locus_tag="VFV99_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062703.1"
                     /translation="MGDPVPDSKTPALAAVGMVVLGLVIAAMQGLSHGSIAGGVIAAL
                     GAGPACFGMWKGIQQETQGTLAISVGAVLLALAVGGLLIVLRVIHWVT"
     gene            726..1757
                     /locus_tag="VFV99_25200"
     CDS             726..1757
                     /locus_tag="VFV99_25200"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5062704.1"
                     /translation="MRALLAICLLCATAAAAPGRLQYTDKAMGTYVTVWFWTDKEAEA
                     AKTAESVFKEMRRLDAELTTWTDTSEVSKINTAAGTGKPVAVSAETFEIIERAQDVAK
                     KSGGVFDISVGAFKGMWKFDEDMDGSLPDPKDVKKRLALVGYKDIVLNKAKKTVLLKR
                     KGMSITLGGIAKGYAVDKCVALIKKAGFNDFMVQAGGDMYISGKTAGRTDGSQNWVVG
                     IRDPRGKEGDLFAGMPIENHSFSTSGDYERGFVKDGVRYHHILDPRDGQPAHASRSVT
                     VRAKDAYTADAWSKVMFILGWKAGMDTIKKYKLTDFEVVWVDDKNEVHMTPTIEKEIK
                     IVHQPTPGP"
     gene            complement(1758..2093)
                     /locus_tag="VFV99_25205"
     CDS             complement(1758..2093)
                     /locus_tag="VFV99_25205"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5062705.1"
                     /translation="MDGTPGTGREGSSAQAVARVVYSSNALANIERVLDSFTDSPASA
                     LRAVTAIHSAVAMLEHHPLIGRIVSDEVRELVVSFGRSGYLVLYEHISALDQVWTYAI
                     RHQRELDYP"
     gene            complement(2033..2317)
                     /locus_tag="VFV99_25210"
     CDS             complement(2033..2317)
                     /locus_tag="VFV99_25210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062706.1"
                     /translation="MTTKATTLKLPAKLKTRIARMAKKSGRTPHAFMLEALERQTTRE
                     ERMEEFVKEAMQAKRDIEGGGEVYAAEDVHAWMERLARGEKAPRPKPWRG"
     gene            complement(2378..3445)
                     /locus_tag="VFV99_25215"
     CDS             complement(2378..3445)
                     /locus_tag="VFV99_25215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062707.1"
                     /translation="MRLSLALLACAGCSTYIQHRAALVPHATPVQALGQPAAWKGNAT
                     LGATNIADAVAPGVGDNPNAGVAIPSSQLRGALNLAVTRNLTIGIFSERGLASTAHPI
                     KDTLPPLDDRGVGGVGYSASYSIPTGSPWRIGLALEMTMWRVPWVEYSQCIDFCTTPT
                     VTVSKGTTDVGQIGFGIVPSYQFGDWTFFGGLTGRTHPLIEEKVINNGEWPDVEEGPM
                     NAIVSGGAAYEAGERVQFVLQAHQVLTQDPVAYGPSIGFSVVIGLGPRYPKVDPPPPA
                     PVMYVPVGATPAGPGALPPPVTLDAHSQAVELTQQARTQAQAGECDDVKAIDARVRAL
                     DPEYHATVFMRDALIAYCVAP"
     gene            complement(3530..4855)
                     /locus_tag="VFV99_25220"
     CDS             complement(3530..4855)
                     /locus_tag="VFV99_25220"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5062708.1"
                     /translation="MSVAVSPSLPVASIVFTDEVSPHLTIQRYTLPCGLDVILHPDGA
                     VPQVCVSVWYRVGSSDETPERTGFAHLFEHVFKNSLHMPAHHYDVLRRAGASEANAST
                     SSDRTAYYEVVPANELSLALWLESDRMGYFLPGMEGERLAQQQAVVRAERRQRYEDTP
                     YGPERFALAEAMYPEGHPARHLTIGLHEHIQAATLADVQDFYRAWYVPANATLVIAGD
                     IDVAEARALVERYFGSFPRSTRPVRPMPVTPPIAGPHRGQVYDKFAALTRIHRAWHGP
                     LAFADDEPELDVLSSAWCAVGTGALWRKLVYETQLAQRVSAWTLNGRIGGEFHVAVDL
                     RTGSDPAAVRAILDEECARGIEPDAIARQITRREASCVWSLASLSRRANLLQRYALYT
                     ENPDGLADDLARYRLVTPATIAAALQRWLSPERMVEIETIPQAKPGASQ"
     gene            complement(4852..5709)
                     /gene="rsmI"
                     /locus_tag="VFV99_25225"
     CDS             complement(4852..5709)
                     /gene="rsmI"
                     /locus_tag="VFV99_25225"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5062709.1"
                     /translation="MSVEGTLFIVGTPIGNLEDLSLRAARVLREVDLIAAEDTRSAKT
                     LLAHVDALGAVNPVRRVMSYFEGNEAEREEDIYDALAQGRDVAIISEAGMPGISDPGA
                     RAIAGAIEVGARVEVIPGPNAALAALVASGLPTDRFTFLGFPPREAGARQELFGTLRS
                     EVATLIFYEAPDRVAATLTDLGAALGTDRRASIGRELTKLHEEHVRGTLAELAARYAN
                     EPPRGEVTLVVAGGSAAAPAVDIEAELRRLLDSGLGPKDAAARLVVVTGKPRRQLYQL
                     ALSLQREKS"
     gene            complement(5711..>6391)
                     /locus_tag="VFV99_25230"
     CDS             complement(5711..>6391)
                     /locus_tag="VFV99_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5062710.1"
                     /translation="RNLLDLVQEGNIGLIQAVSKFDPYRGVKLSSYAAFWIRAYILKF
                     ILNNWRLVKIGTTQAQRKLFFNLRKEREKLEQLGFQPTAALLAEKLDVPEKEVIEMER
                     RLAAPEASLDAPLGGGDDDGMRTRLDYLPSEDDRPDRAVAQSEFSELLRGKLEAFANT
                     LEGREQTIFRERWLTDEPLTLQEIGDRYNVSRERARQLEKRMLDRLKKYLEAEMGTAV
                     DIEALTRD"
BASE COUNT         1099 a         2046 c         2167 g         1079 t
ORIGIN      
        1 gacgaggtcc gggcaccgcg caaaatcccg ttagcaacac gcgcggcatc gcaactccaa
       61 tggtccggca actcgtcggc gcgtgcaagc ggagtcgttg ccttgcttga ttcagaccgc
      121 ctcagtgcgt tatctcgtcg aacgtgaatc gcctgatcga ctaccttcga caaaatattt
      181 tgatcgactt ccagggtgat ctgtcggtgg caaaggtgag ggagttactg gccggcgacg
      241 agagtcgcga tgcaaaggca ctactgacga aattggtggc tgaccgcaaa gttgaagaca
      301 tgctcgtcgt ccttgccgac tgcctgacgg aaccagtcca aaaggcgcta accgacgatg
      361 tcatgcgcga acaactgcgg atgtactccg agagctgaac gcggccttga ctcgacagac
      421 cccgtgctac gttccacggc atgggtgacc cggtgcctga ttcaaagacg cccgcgctgg
      481 cggccgtcgg catggttgtt ctcggcctcg tgatcgcagc gatgcagggc ctcagccatg
      541 gctcgatcgc cggcggcgtc attgctgcgc tcggcgcagg ccccgcgtgc ttcggtatgt
      601 ggaagggcat ccagcaagag acgcaaggca cgctcgcgat ttcggtcggc gccgtgctgc
      661 tcgcgctcgc ggtcggcggg ctgctcatcg tccttcgtgt cattcactgg gtgacctagg
      721 tcgcgatgcg cgcgctgctc gccatttgtt tgctgtgcgc gaccgcggct gcggcgcccg
      781 gcaggctcca gtacaccgac aaggcgatgg gcacctatgt cacggtctgg ttctggaccg
      841 acaaggaagc cgaggccgca aagactgccg agtccgtctt caaggagatg cgccggctcg
      901 atgccgagct gacgacgtgg accgatacgt ccgaggtctc gaagatcaac accgccgcag
      961 gcaccggcaa accggtcgcc gtctcggccg agacgttcga gatcatcgag cgcgcgcagg
     1021 acgtcgcgaa gaagtcaggc ggcgtgttcg acatcagcgt cggtgcgttc aagggcatgt
     1081 ggaagttcga cgaggacatg gatggctcgc tgcccgatcc gaaggacgtc aaaaagcgcc
     1141 tcgcgctcgt cggctacaag gacatcgtcc tgaacaaggc gaagaagacg gtgctgctca
     1201 agcgcaaggg catgtcgatc acgctcggcg gcatcgcgaa gggctacgcg gtcgacaagt
     1261 gcgtcgcgct gatcaagaag gccggcttca acgacttcat ggtgcaggcc ggcggcgaca
     1321 tgtacatctc aggcaagacg gcaggtcgaa cagacggcag tcagaactgg gtcgtcggca
     1381 ttcgcgatcc gcgcggcaag gaaggcgacc tgttcgccgg catgccgatc gagaaccact
     1441 cgttctcgac gagcggtgac tacgagcgcg gcttcgtcaa agacggcgtc cgctatcacc
     1501 acatcctcga tccgcgcgat ggccagccgg cacacgcgtc acgctcggtg acggtccgcg
     1561 cgaaggatgc ctacacggcg gacgcgtgga gcaaggtgat gttcatcctc ggctggaagg
     1621 ccggcatgga cacgatcaag aagtacaagc tgaccgactt cgaggtcgtc tgggtcgacg
     1681 acaagaacga agtccacatg acgccgacga tcgagaaaga gatcaagatc gtccatcagc
     1741 cgacgcctgg gccgtagtta cgggtagtcg agctcgcgct ggtgtcggat cgcgtaggtc
     1801 cacacttggt cgagcgccga tatgtgctcg tacaacacca ggtagccgct ccgcccgaac
     1861 gacacgacga gctcgcgcac ttcgtcgctc acgatcctgc cgatcagagg atggtgctct
     1921 agcatcgcga ccgcagaatg gatggccgtg accgcgcgga gtgccgaagc gggcgaatcg
     1981 gtgaaggagt caagcacgcg ctcgatattc gcaagcgcat tgctcgaata aactacccgc
     2041 gccacggctt gggccgagga gccttctcgc cccgtgccag gcgttccatc cacgcatgca
     2101 cgtcctcggc agcatagacc tcaccgccgc cctcgatgtc gcgcttggcc tgcatcgcct
     2161 ccttgacgaa ctcttccatg cgctcctcgc gcgtcgtctg ccgctcgagc gcttcgagca
     2221 tgaatgcatg cggcgtacgg ccgctcttct tcgccatccg agcgatgcgc gtcttcagct
     2281 tggcaggaag cttgagcgtc gtggcttttg tcgtcatgac atcaccgtgg gtactactaa
     2341 agtaccacca cgccgtagta ggcgccaggt tgaccgatta tggcgccacg cagtacgcga
     2401 tcagcgcgtc gcgcatgaac acggtcgcgt ggtactcggg atcgagcgca cggactcgcg
     2461 catcgatggc tttgacgtcg tcgcactcgc cagcctgcgc ttgtgtgcgc gcctgctggg
     2521 tcagctcgac ggcctgcgag tgagcatcga gcgtcaccgg tggaggcagc gcgcctggac
     2581 cggccggcgt ggcgccaacc ggaacgtaca tgacaggcgc gggcggtggc ggatcgacct
     2641 tcggatagcg cgggccgagg ccgatcacga ccgagaagcc gatgctcggg ccgtacgcga
     2701 ccggatcctg ggtgagcacc tggtgggcct ggagcacgaa ctgcacgcgc tcgccggctt
     2761 cgtaggccgc accgccggag acgatcgcgt tcatcgggcc ttcctcgacg tcaggccact
     2821 cgccgttgtt gatcaccttc tcctcgatga gcggatgcgt gcggccggtg aggccgccga
     2881 agaacgtcca gtcgccgaac tgatagctcg gcacgatgcc aaaaccgatc tggccgacgt
     2941 cggtcgtgcc cttcgacacc gtgacggtcg gcgtcgtgca gaagtcgatg cactgcgagt
     3001 attcgaccca cggcacgcgc cacatcgtca tctcgagtgc gaggccaatc cgccagggcg
     3061 agccggtcgg gatcgagtag ctcgccgaat agccgacgcc accgacgccg cgatcatcga
     3121 gcggcggcaa cgtgtccttg atgggatgtg ccgtcgaagc gagaccgcgc tcggagaaga
     3181 tgccgatcgt caggttgcgc gtgaccgcga ggttgagcgc gccgcgcagc tggctgctcg
     3241 gaatcgcgac accggcgttc ggattgtcgc cgacacctgg cgccactgca tcggcgatgt
     3301 tcgtcgcacc gagtgtcgcg ttgcctttcc aggcggctgg ctgaccgagc gcctgcaccg
     3361 gcgtcgcgtg cggaacgagc gcagcgcggt gctggatgta cgtcgagcaa ccggcgcatg
     3421 cgagcaaggc gagcgagagc cgcatggaaa gaggatcgca cgtccggcgc aggcaggcgg
     3481 tggcacagca cacatgccgc ccggcgctgc cggcgtttgc cgttcgcgat tactgcgatg
     3541 cgcccggttt tgcctgcgga atcgtttcga tctcgaccat gcgctcgggc gagagccagc
     3601 gttgcaatgc cgcagcgatc gtcgccggtg tgaccaaccg atagcgcgcg aggtcatcgg
     3661 cgaggccatc gggattttcg gtgtagagcg cgtagcgctg caggaggttc gcgcggcgtg
     3721 acagtgacgc gagcgaccac acgcatgacg cttcgcggcg cgtgatctgg cgtgcgatcg
     3781 cgtcgggctc gatgccgcgc gcgcattcct cgtcgaggat cgcgcgaacc gcggctggat
     3841 cggagccggt gcgcagatcg accgcgacgt gaaactcgcc gccgatgcga ccgttcagcg
     3901 tccatgcgga cacgcgctgg gcgagctggg tttcgtagac gagcttgcgc cacagcgcgc
     3961 cagtgccgac ggcgcaccat gcggaggaca gaacgtcgag ctcgggctcg tcgtcggcga
     4021 acgcgagcgg accgtgccat gcacgatgga tgcgcgtgag cgcggcgaac ttgtcgtaga
     4081 cctggccgcg atggggaccc gcgatcggcg gcgtgaccgg catcgggcgc acgggccgcg
     4141 tggagcgcgg aaagctgccg aagtagcgct cgacgagtgc gcgcgcttcg gcgacgtcga
     4201 tgtcgcccgc gatcacgagc gtcgcgttcg ccggcacgta ccaggctcgg tagaagtcct
     4261 ggacgtcggc gagcgtcgcg gcctggatgt gctcgtgcag accgatcgtg aggtgacgcg
     4321 ccggatgccc ctcgggatac atcgcctccg cgagcgcgaa tcgctcggga ccgtatggtg
     4381 tgtcctcgta gcgctgacgg cgctcggcgc gcacgaccgc ttgctgctgc gcgagacgct
     4441 cgccctccat gccgggcagg aagtagccca tgcgatcgga ctcgagccac agcgcgagcg
     4501 atagctcgtt cgccggtacg acctcgtagt acgcggtgcg atccgagctc gtcgatgcgt
     4561 ttgcttcgga cgcgccggcg cggcgcagca cgtcgtagtg atgcgccggc atgtgcagcg
     4621 agttcttgaa cacgtgctcg aacaggtgcg cgaagccggt gcgttcgggg gtctcgtcgg
     4681 agctgccgac gcgataccag acgctgacgc agacctgcgg caccgcgccg tcagggtgca
     4741 gaatcacgtc gagcccgcac ggcaacgtgt agcgctggat cgtgaggtgc ggcgacacct
     4801 cgtcggtaaa gacgatcgac gcgacaggca gcgatggcga gacggcgacg ctcacgactt
     4861 ctctcgctgc aatgatagtg cgagttgata gagctggcgg cgcggttttc cggtcacgac
     4921 cacgagccgt gcggcggcat ccttgggccc gagcccgctg tcgagcagac ggcgcagctc
     4981 ggcctcgata tccaccgcgg gcgccgcagc gctgccaccg gcgacgacga gcgtgacctc
     5041 gccacgcggt ggctcgttcg cataacgggc ggcgagctcg gcgagcgtgc cgcgcacgtg
     5101 ctcctcgtgc agcttcgtca gctcgcggcc gatcgatgcg cggcggtccg ttccgagcgc
     5161 ggcaccgaga tcggtgagcg tcgcggcgac gcggtccggt gcttcgtaga agatgagtgt
     5221 cgcgacttcg ctgcgtagcg tgccgaacag ctcttggcgc gcaccggctt cgcgcggcgg
     5281 aaagccgagg aacgtgaagc gatcggtcgg caggcccgat gcgaccagcg cggcaagcgc
     5341 ggcgttcggc ccaggaatca cctcgacgcg tgcgccgacc tcgatcgcgc cggcgatcgc
     5401 gcgcgcacct ggatccgaga tgcccggcat gcccgcctcg gagatgatcg cgacgtccct
     5461 gccctgcgcg agcgcgtcgt agatgtcctc ttcgcgctcg gcttcgttgc cttcgaagta
     5521 cgacatcacg cgccgcactg ggttgacggc gccgagcgcg tcgacgtgcg cgagcagcgt
     5581 cttggcactg cgcgtgtcct cggcggcgat caggtcgact tcgcgcaaca cgcgcgcggc
     5641 gcgcagcgac aagtcttcga gatttccgat cggcgtaccg acgatgaaca gggtgccctc
     5701 gacgctcacg ctagtctcgc gtcagcgctt cgatgtcgac tgcggtgccc atctcggcct
     5761 cgagatactt cttgagccga tcgagcatgc gcttctcgag ctggcgcgcg cgctcgcgcg
     5821 agacgttata gcgatcgccg atctcctgca gcgtgagcgg ctcgtcagtg agccagcgct
     5881 cgcggaagat cgtctgctcg cggccttcga gcgtgttcgc gaacgcttcg agcttgccgc
     5941 gcaacagctc ggagaactcg ctctgcgcga ccgcgcgatc cggccgatcg tcctcggacg
     6001 gcaggtagtc gaggcgcgtg cgcatgccgt cgtcgtcgcc gccgccgagc ggcgcgtcga
     6061 gcgaggcctc gggcgccgcg agccggcgct ccatctcgat gacctccttc tcgggcacgt
     6121 cgagcttctc ggcgagcagc gccgcggtgg gctggaagcc gagctgctcg agcttctcgc
     6181 gctccttgcg caggttgaag aacagcttgc gctgcgcctg cgtggtcccg atcttcacca
     6241 ggcgccagtt gttcaggatg aacttcaaga tgtaggcgcg gatccagaac gcggcgtagc
     6301 tcgacagctt cacgccgcgg tacgggtcga acttcgagac ggcctggatc agcccgatgt
     6361 tcccctcctg cacgagatcg agcaggttcc t
//
LOCUS       DASUFX010000629        34485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_379022,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000629 DASUFX010000000
VERSION     DASUFX010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 34485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34485
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379022"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="VFV99_25235"
     CDS             <1..1699
                     /locus_tag="VFV99_25235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062711.1"
                     /translation="PGADEAAFDEDLTFTPPRTNTATQSMPPPTRTPAPIGVVKSGGT
                     SPGTPAPTSRTPDLATPPFATPIARSGVGTGPRPVVSPPTTIPSIATRPAGTSTPLAT
                     PLSARSTVATPPPSPVVPRPASPTPSTNPALPRQTANTPVTNPTVPRTEVTPLPTPPV
                     SAAESPEPSGSLDISPSMMLDVEPLRRAPTAPPPPPVKRPPTAPPASVEADPEPSSDA
                     PTRPTPQPNAAELWARSMAPAESPKPTLADSTESTMANLFATQTVRKRPPTPPPAHLE
                     TLGSPFAPGQLPEGDFSYPIKRSGFGVLLKIVLGLGVLALGFFAFVKFYPMDDNTPTQ
                     TAAVTPPAPPEPAPAPVVEAPAVVVDAPPATPPAPIETEQPPTPTETPAPPTPPTPTE
                     TAKPPTKPTTKPATPTTTKPATKPTTETGSSPTTESTPTPTHTETADTKPESSGGDCD
                     EVGCVISKYDRPCCAKYKPSDTFTPKNVVPDELDRAMVKAGVEKIKPRVVACGEKNSN
                     KGTVRLAVTVDSEGHVKTVTVSETPDTALGECVAAAMRAATFGKSVNGGEFNYPFVF"
     gene            1810..2076
                     /locus_tag="VFV99_25240"
     CDS             1810..2076
                     /locus_tag="VFV99_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062712.1"
                     /translation="MALALQMRSRMQTLNILDLEVVTGGGDVRAPLSGDVTQQINTNW
                     GGTQIINPPPAPPKKPSGLRIDYLREHPEARWGAPPYVNRPVRR"
     gene            complement(2516..3454)
                     /locus_tag="VFV99_25245"
     CDS             complement(2516..3454)
                     /locus_tag="VFV99_25245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062713.1"
                     /translation="MRVGAFIMMAALVGCGEPTALPCADNPTCSGGLCIDGFCADQDT
                     ACQSGYRYGDSAGDRAGDCTGGGAGSGSGNAGPDMIYTLTNDGAPTVVDASNAHDDVK
                     PQCAGVGGVDVMFDVSVPSGLTRLYVDTSQTDYDVVLAIYDGPCASSAKTELACIASG
                     AGSCDARTKQWSEILNPGAYCVVIDQPAAGASHVATARAIIGPPSIAGRIGANSATTC
                     GHNDWYPSTGCIVQSDVEDTSWFFMGCSGTWKATAGMAWVGDLEVFLPLGEPYGCADR
                     VTGITYPQTKPGPFWLVAHQDAGQSCGTLTATITKN"
     gene            complement(3451..4206)
                     /locus_tag="VFV99_25250"
     CDS             complement(3451..4206)
                     /locus_tag="VFV99_25250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062714.1"
                     /translation="MRCFLISVLLVAGATGTAIADRKLSDAANAELERGEASFRAKDY
                     AAAIKAFDAGYAIDPQPIFLYDKAQAQRLAGDCKSAIDTYKQFLATEPPANEATRAKK
                     NLENCQTTLPPPVEVAPAPAREEPQKAAEPEPEPAREPPPPSHTETSRWWRDSVGMSL
                     LTTGVIGLGIGAGFAVAARAAASDTALATNVDQWTVARDRWERDRIVAGVALGAGATC
                     VVLGVLRLSLHERSVTVTPARGNGAVVTLGGVW"
     gene            complement(4187..5941)
                     /locus_tag="VFV99_25255"
     CDS             complement(4187..5941)
                     /locus_tag="VFV99_25255"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062715.1"
                     /translation="MSRRYEIVCRLAGGGMADLYLARTLGPGGFERLVVIKRIAKRLA
                     SQASAVQALFDEARIAATLSHANIVQVNDIEIADGQVSIVMEFLHGHDVSHLLRRMKR
                     ANEHVPLDQAVAITQGVCAGLHHAHERMDSEGKSLAIVHRDVSPHNVFVTYDGAVKLV
                     DFGIARASMRKDHTDHGFIKGKPGYIAPEQIKGRKTDRRTDVWGAAVLLYELTTGRTP
                     YGISTAFEELVKVTKEDPPPPSSHVRGYPLELEAIVLRGLARDPAQRYSTAEEMRVAL
                     DEFARNRGLDLSPFRMAALMERVFAENLEAWRQAQRLGKTLAEHVAAFKTSGAHDIVA
                     LPGDPTEPFESFDNTHPVGTSDFASRPITAPTRLLRPSANKPSAFAGPTEITERTPTT
                     KRFPAPAVPAPPDPAPVLEVKATTSTPRYDTSRPTPMAFESLQFGEATTVPREKHSPF
                     MLWRTRIALGLAAPLVVILSMVAWRSLDANSSPAPQPAEQPRATAVVDEPVAQPMISV
                     IPIESEPEATKPAASEPSIEISTAVAHASPSPARKRPSAKPTPKKKPVRTVQPTRAVP
                     APPPPQAAEDDLDALLPH"
     gene            6041..7315
                     /locus_tag="VFV99_25260"
     CDS             6041..7315
                     /locus_tag="VFV99_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5062716.1"
                     /translation="MRVIDGPDRGTVVHDADGEIAIGTADGNTLVLTDPTVSRYHVLI
                     TSSDKGAHLRDVGSTNGVFLGEHRVESGWIDTGAVLKLGLTTLRFEYGEDDAEQPLSA
                     DDYFGRAIGRSIAMRRVFEALPRIASSDATVLIQGETGTGKTLLAEAIHQAGPRARAP
                     FIVIDCGAIPPTLIESELFGHEKGAFTGAAGARVGAIESAAGGTVFFDEIGELPLDLQ
                     PRLLRVIEDRMIKRVGGRDQIRIDVRVVAATHQDLRAAVNRGDFRSDLYYRLNVVSLR
                     LPPLRERRDDIPLLVESFYEQFAGHAGAPQDLIDELGRHDWPGNVRELRAAVERAVLL
                     GDPALWQAITDETRGDVVPAVANEPFDDSLSFRAAKERALVRWERGYIAELLRRHNGN
                     LSRAARAARMDRTHLRELGRRYHLLPSVTDDE"
     gene            7525..8016
                     /locus_tag="VFV99_25265"
     CDS             7525..8016
                     /locus_tag="VFV99_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062717.1"
                     /translation="MRWVSPEHLRVTHGEIREGRIVSRAMRAVALASRGDAASLVFTL
                     HGIVFDPTPKLGVELRAANTSHLVYVMWRLIPKTRLTVQVKAPDASYLRVKPIHSELL
                     DVPELGRAQKLAARIEGDRLSAWIENHLVWMGVLPPPARALVGFAGMRADHVAADVAL
                     AAD"
     gene            8067..9497
                     /locus_tag="VFV99_25270"
     CDS             8067..9497
                     /locus_tag="VFV99_25270"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEX5062718.1"
                     /translation="MKCCLLIFVCIACGPSNHHGSDCTGADCSTGSCNPGDSRDCYTG
                     EAVTKDVGPCHGGNQLCTGAGQWGECMGQVIPAGESCTDGLDNNCNGMVDEMTDLDGD
                     GFATCDSSGASLDCCDSVECGTPAAVNPGAYDVAGDSVDNDCDGTIDNEVGLCDQSLA
                     SNSQAASDYAKAIDICQNATPTDKKWGLIEAKLTLPSGTGVPDPQSYSIRHKFGSKVL
                     PQGGSSVAVISTGGAAAKGDINPPYHDFVSYETTKTSPFPADFLAANGGSLPNAPGCP
                     EPVGAVANDPVMLTFRIRVPTNARSFSLRSNFYSSEFPEWTCSPYNDFYVILIDSTST
                     DNPTDKNLAFYSPPNAMAKVPVGVNLGFGNTGLFTQCVNGATGCATMGMGQAGTISTC
                     AGTDDLMATGFDDPAPMECEAASLKGGATGWLTTSGNVVPGEIMTLRIAIWDTSDHAL
                     DSLAVVDGFKWSADPSTPGTVILKTH"
     gene            9626..10189
                     /locus_tag="VFV99_25275"
     CDS             9626..10189
                     /locus_tag="VFV99_25275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062719.1"
                     /translation="MSTTLHRLCVAAEQAATRGDHHSAVRNYIAAGDAARNRERWQLA
                     ARCYRSALEIDLGSRVAVSRLVHIGGHTNDRRFWVEYSRMLKHDASWSQLTCRRAQIL
                     VHNLGSVVECAEVGPVIELVIASDGSFLCLPDARFRAMPLAMTLIIMRRALWPTGRSS
                     RAAPRVRLGYRGHTVWLNARGDWSLGD"
     gene            complement(10186..10647)
                     /locus_tag="VFV99_25280"
     CDS             complement(10186..10647)
                     /locus_tag="VFV99_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062720.1"
                     /translation="MHAEGSSELRVTTSATGTFSVVAPADQALEVEAYGRRGGVARIT
                     VPAKGPSSDIVLHFAGTATLEGAITLGDDPISGAMIQLRNGTSSDRPYALAETDASGY
                     FKRQTLEPGSYELSIVRNDSNSSEPIKYSTAVEVKAGANYANTDLTKVQPH"
     gene            10680..10868
                     /locus_tag="VFV99_25285"
     CDS             10680..10868
                     /locus_tag="VFV99_25285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062721.1"
                     /translation="MHATVVAHFATPERAASAAKQTFGGLVANLRSTVHDRDVTFTVE
                     LDATQTAQVIDQLTALMR"
     gene            10871..11323
                     /locus_tag="VFV99_25290"
     CDS             10871..11323
                     /locus_tag="VFV99_25290"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX5062722.1"
                     /translation="MAWVKIPAEHHALLIAALPKDPRVRTINMFGGTAALVNGNMFGG
                     TFARSVIVKLGPDDYPEALALDGAEPFDPMGRGHVMSNTVLLPETVMDETAELKSWLA
                     RAFTYTATLPPKKKGAAKKPAAKKVPAKKLGAKKISARKPAAKRKKRS"
     gene            11364..12452
                     /locus_tag="VFV99_25295"
     CDS             11364..12452
                     /locus_tag="VFV99_25295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062723.1"
                     /translation="MDSDVVEVVARFGGTILDVAHVGPADTYRIGTAQGTNLSVPGLT
                     CFPLVTGGKVRHPVGVPVVERDGWTEIRVGALSIHITRTKLARAPLARPRIQWRTPAF
                     LAASLVVHIAIWFVALLHAPIEKLPAQKPRPRLVHVEVAPPEPPPKPAPHEATENERK
                     KASVAAVQPSRRPRVPREERDERITTTEAAVAAMARSFDAIDLAGKMADLRPEDTYRE
                     DEANARGFGGGGTGRRFDPGPAESIASGPYATMIFDVKLCPNKACTVEGPVPALFVRT
                     HLLAHMDEIYACYMEHASEPGTIVLSFTITPDGAVRDARGTGLGETGPCAARVVGEIF
                     FKALGNDYVPPRSTYVRRYPVRFNPARG"
     gene            complement(12449..13675)
                     /locus_tag="VFV99_25300"
     CDS             complement(12449..13675)
                     /locus_tag="VFV99_25300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5062724.1"
                     /translation="MTDLAWIDAALISARPQALGALVRYFRDVDLAEEAFQDASLRAL
                     KSWPENGPPRNPTAWLILVGRNIAIDQTRRKKKQTDLPDDDVAAESESSLAERLDDAH
                     YRDDVLRLLFICCHPDLPPSQQIAVALRIVSGLTVKQIARAFLVTEAAMEQRITRAKN
                     RIAAADVPFETPDAHERAERMSAVAAMIYLIFNEGYSAGREDADRAQLCDEAIRLGRL
                     LIRLFASEPEIMGLTALMLLQHARAAARFDDDGAVVLLDVQDRTKWNRNLIAEGLAML
                     DKALRHAQPGPYQIQAAVAALHSRAATPADTDWAQIDLLYANLERLQPSPVVTLNRAV
                     AVSKTKGAAAALAMIEPLETKLAGYFHFHGLRGALLQQLGRDREARAAFTQAISLANT
                     AAEAAHIRMHLDQLTR"
     gene            complement(13672..14043)
                     /locus_tag="VFV99_25305"
     CDS             complement(13672..14043)
                     /locus_tag="VFV99_25305"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5062725.1"
                     /translation="MLYAILCYDSEAVTCAWSKEKDAEVMASLGKVISKIGEQKRLGP
                     VARLMPTTAATTLRKGKDAMVMDGPFAETKEQLLGFYVIEAASLEAALADAKELAAAS
                     GSAGAFEIRPLMLYQPGGPIA"
     gene            14152..15117
                     /locus_tag="VFV99_25310"
     CDS             14152..15117
                     /locus_tag="VFV99_25310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5062726.1"
                     /translation="MASKLEQSELYGLKALRGVSLDSVEELLGTCELRRLAPGDVLLT
                     LGQPNDTMYMILSGELSIHLEGPTSEAVATLGPGETVGELSVIDQSPASAYVVAAEPT
                     RLLAIDEPRFWNLVNASHDFAINLLLSLAKRLRANNSTVSTNIKLQREYKRNALIDGL
                     TGLYNRRWIDEALPRFVQRYGRGGQPLTVLMIDVDHFKRFNDTYGHAMGDQVLVRVGQ
                     ALRASLRPTDHVARYGGEEFLVILPDTVGDAAEIVANRLRTAVSDIDLVQADGTAIPR
                     ITISLGGGSLRSGQTMKALLGHADEALYQSKANGRDRVTFAVSSS"
     gene            15114..15881
                     /locus_tag="VFV99_25315"
     CDS             15114..15881
                     /locus_tag="VFV99_25315"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5062727.1"
                     /translation="MTDAIDPVKRASFDGMAARYDAARPSYPSAVVDDVLARSGAKRA
                     LEVGAGTGKATVLFGQSGLDIVALEPGGQLAALLRERVRGMRVEVVESMFEACDLTGF
                     DLVYAATAFHWVEPSVRYVRAAEALRPGGALALIMNEKAPMDPTTRDDFNAAYAKHFG
                     WPQWNPQYLERTEASWVDEINASGRFGPVHVGRFPWSATYTTDEFVALLDTYSDHVVQ
                     PDDKRLPLYDDIRAAIDRRGGSVEIPYLTFEFFALRL"
     gene            15883..16479
                     /locus_tag="VFV99_25320"
     CDS             15883..16479
                     /locus_tag="VFV99_25320"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEX5062728.1"
                     /translation="MRVVVYDKTCVKTRGRLSPIWATGARLYRALRRADAVHGVASWG
                     EALAWLASHKQPLREIQYWGHGKWGRALVGEESLDATALTTRRAELEAVRERLAPDSL
                     IWFRTCETLGAKPGIDFAERLADFFGARIAGHTYIIAFHQSGLRGVAPGCRADWSPDE
                     GVAAGTPEAPEAAHWSRPWRPRTVTALHGAVPAAWITR"
     gene            16507..17280
                     /locus_tag="VFV99_25325"
     CDS             16507..17280
                     /locus_tag="VFV99_25325"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5062729.1"
                     /translation="MVRRTLVLIVVTACGSEHDPVMPDAAGNPSIAGTPVSSFETSSC
                     STGDVLALSIQIADEVHCIMPGQLVAFEEGPAIMFTGSAVLPYVSSAARDALYAATSG
                     SGNVLQVTSGFRTVVQQYLLYRWFQMGRCGITAAADPGTSNHESGRALDVSNYNTWIT
                     ALEANGWAHDVPGDPVHFDHLSSPDIRGADVLAFQRLWNRNAPDDPITEDGQYGPMTE
                     ERVKRAPAEGFGIGAPCAQRLAPPIARSGEGNDEVECAR"
     gene            complement(17288..18811)
                     /locus_tag="VFV99_25330"
     CDS             complement(17288..18811)
                     /locus_tag="VFV99_25330"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062730.1"
                     /translation="MRVVLSFVLILAACGDNGGGGDDMMPGPCTPPVAPLPTTGMYID
                     PNAAELPSNCVADGLRDLPGRWFVSDQAQYFRFEYPKYEGSCTTGFSRLGNTDDHDAS
                     DGYSFFTWSDGTRYWQREAYEYGNTVFIRVYAACLTSSDTLAATYITYDTDRGERISR
                     TTGERFAAHKEDSLATGLTLVGSLEKGLPGEVIEGLNLMIDGTHAYLAGIGGLEIIDI
                     SDPTAPVAVGHYDGSWNDVRVVNDGTNVVAFLSPFGSEDPTQVVNVTDPAAPTFVSML
                     QEYSHSLQIQERNGKKELYLATYNESVPKYDVTAPLTPVRQGMAIVPGDVSGVHDLYV
                     DGDKIYACNTTLGLVGFDTSGGFGTSNVELGRYRLGYSHAAWAGTIGGRKIVLAGDEG
                     MTASPSGGAHLSILDGDTASPTFMHELAIYQTRREVGIHYFEVHGNKVYIAYYHDGVR
                     VIDLTDPTHPVEVAHYNDWIEDNAYGGAFEGALAVRKIGDLLYVADLERGLLVLREQ"
     gene            complement(18918..20036)
                     /locus_tag="VFV99_25335"
     CDS             complement(18918..20036)
                     /locus_tag="VFV99_25335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062731.1"
                     /translation="MICPACSTALDSRATFCPTCGRTVDDPHIGLVIAGHYLVKKRIA
                     IGGFGSIYRAEQTDVGQPVALKIMHRELAADDKLVARFRREGEVLIKLRDPHTVATYE
                     IGSTPEGLPFIAMELLEGETLLRLLQLHGRMPWNRVFGIARQMCSALGEAHALGVIHR
                     DLKPGNIFLTNDDVVKVLDFGIAKILATSDMPPPQELTIMGTAVGTIEYMGPEQLMGG
                     RADNRTDIYTVGVLAYELIVGRRPFNAVGLDLLTQQLTEAPPPPSTLVSVPHVVDEVV
                     LRCLAHDSEERFANVQALASALDAALASYRPEPAMPAFAEPPPRAASALPAGRPVMRA
                     EPTEKFPRDQASRSSVVMLVVAIMLFVLGIGLVMAYLY"
     gene            complement(20033..20884)
                     /locus_tag="VFV99_25340"
     CDS             complement(20033..20884)
                     /locus_tag="VFV99_25340"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX5062732.1"
                     /translation="MSEVFLFGATSMLGWSILRAGKASSVTAFCNALTREVPDGIAEG
                     IDLDDEPAVAALFARSRMMTPRVIINCAGVCDVGTCEDAPEFAHTVNVEGTRVLLAHA
                     PASARIVHLSSDHVFSGDTGPYYEDSPTDPVSVMGQTRVAAEQLVLARDNTLVIRAGL
                     WIGPSATGRTGHLDWLRGRTQRGLPMTVVADEHRSAVWAEDAARRVWQLVHSDVTGVR
                     HIVATKIVSRPVLARYLNERFAIGAQLGFEARAERAHGRPHLGRIDLRTRYLDALAAP
                     LPSVVPA"
     gene            complement(20895..21431)
                     /locus_tag="VFV99_25345"
     CDS             complement(20895..21431)
                     /locus_tag="VFV99_25345"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5062733.1"
                     /translation="MNATNVLVRMAHNNAWANLRLHRAVGELDDAEYRATRTSFFPSL
                     HLTLTHILFVDLFYVDALEAGGRPRPWAALEAFEKSGTFAELRRMQRDVDMRLVACAA
                     GNPEDEIRMQRETRVQTDTRANVLLHLLEHQIHHRGQAHAMLAGTRVKPPQLDEFFLR
                     DELPLREAELRELGLPLG"
     gene            complement(21428..21901)
                     /locus_tag="VFV99_25350"
     CDS             complement(21428..21901)
                     /locus_tag="VFV99_25350"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX5062734.1"
                     /translation="MAGPRSKAELRRELLAQLEAQLATARASHAAATEGAISDDARPE
                     NDKDTRGLEQSYLARGHAQRVADLETAVSVIGAMAIQPLTKVAVGAVVVVEEDDRRRT
                     LFIAAHGGGLVVDGDVTVVTPTSPMGRALHGKVVDDECELTAGGAQRTLAIVEVI"
     gene            21923..22897
                     /locus_tag="VFV99_25355"
     CDS             21923..22897
                     /locus_tag="VFV99_25355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062735.1"
                     /translation="MRYALVISLAACAGCGDNLPECGFVHTLAANRNIWGGHIAVDAD
                     RVYYSDYDNGIGTHLVFRQPREGGQALVISGRDESSRFGYGMVTDDSYLYWTAEVAEA
                     GYTLFATPKLGGASLQLKVVSVCTAHGVAVDSVNSYAGAIRCDDNPAQVVAAPHVGSG
                     GGVIWSSTDADVSALAARDGNVVIATTNGLFRVMPTATELLDGHSTYQVVIVGDEIVY
                     STTEAVIAMPYAGGAQRTLYTFHTTIDQPRPFAVDGDDLYVVESTELVYIHAGDQPIS
                     LVSDMGLAITHIAAGGGAAYWPTLAVPGAPGVLDTFNGAVFRVTPPCH"
     gene            complement(22891..24924)
                     /locus_tag="VFV99_25360"
     CDS             complement(22891..24924)
                     /locus_tag="VFV99_25360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062736.1"
                     /translation="MTAAPRPRDEPVRLATLRDYHVLDNDSEPELDALAKLAAVICST
                     PSAVITFVDKDRVWFKARYQFPATEAPRDLAFCAHAILGPDVMIVPDTTQDERFAETP
                     FVAEAGIRFYASAPLVMANGQCLGTLGVVDTSPRVSLTSVQIEGLAALARQVVAQLEL
                     RKRIVDERRVGHERLQLVTRATNDAVWDWDLRTNAIWWNEGMRTLFGVAPENVSTIDA
                     WSSRMHPDDLPHVERSLFGAIQSGQHYWTDEYRFRCHDGHYANVLDRGYVIHERGQAV
                     RMVGAMLDVTERRRLEEQLRQAQKMEALGQLSGGIAHDFNNLLTVIQVNASLITRAPS
                     TGDAIREHAADIIQASERAATLTRQLLMVSRKQVLQPRIVDVNDVVRNMTRMLQRALG
                     EAITLVAHCSTQLPPVKADVGMLEQVLLNLAINARDAMPQGGQLTIATGEKMITEPDA
                     VRGEDGPRGPHVFITVSDTGSGIAPDALPHIFEPFFTTKDVGKGTGLGLATVYGIIRQ
                     HQGWIDVASDPGRGTTFCFYLPATTARRPQHDARGGDDADLPHGTETLLVVEDEDALR
                     LLVVNLLEKCGYTVYAAQNGAKALEQWPELRDKVQLVLTDLVMPGGVTGRELAERLRA
                     TAPALGIVYMSGYTSAQAGRGEPLVDGVNFLQKPFQPDMLAQIVRAALDRVPQ"
     gene            complement(24996..26183)
                     /gene="purT"
                     /locus_tag="VFV99_25365"
     CDS             complement(24996..26183)
                     /gene="purT"
                     /locus_tag="VFV99_25365"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX5062737.1"
                     /translation="MVSIGTPKSENATRVLLLGSGELGKEVVIELQRFGVEVIACDRY
                     ADAPAMQVAHRSHVIDMLDRAALRAVIEREKPHLIVPEIEAIATQELIALENEGWHVI
                     PTAQAARLTMDREAIRRVAAEELKLPTARYQFADTFDELKAAARELGWPVVIKPIMSS
                     SGKGQSVAKTEADLEQSWHYAQTGGRTGAGRVIVEEFIEFQSEITMLTVRAVNGTHFC
                     DPVGHRQVGGDYVESWQPHTMNAAQIERSQAIARKITDRLGGYGIFGVELFLCADDRV
                     IFSEVSPRPHDTGMVTLATQTWSEFALHARAILGLPIANIRRYAAGASVALRAEGKLA
                     SPVLDGVGEAYATPDIDVRFFGKPNAVPRRRLGVVLATADTAEAALGKAKKVAAGLKL
                     RDA"
     gene            complement(26218..28011)
                     /locus_tag="VFV99_25370"
     CDS             complement(26218..28011)
                     /locus_tag="VFV99_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062738.1"
                     /translation="MRGLCLAICVLAACGSHSGDDSDYMSIEVEPAFTTVTVPLGGTA
                     TQDYKVYGVNGSEKTDITLKCALSMDADFGTFVDATATVLPHGGKTTITAICNTLTGT
                     ALLGVNLVGDVVVPPAPTDAPDLFGNATVGTDAARVPVIEYPLEGAVSPRNMPPIEAQ
                     WTAAGNDLFHISLTSSFVAVNIYTTSPEAQLSEQDWENVMASSAGETLTYTVEGLAQA
                     TPDMKFTSTPRTNVIARDIIDKTAIYYWASSRGNIMEQTFGQTTQNLVKDGCTSCHTV
                     NRSGTRIGYSRCVANDCGNLYTGFLKYDGASQSWMETVNANNLGIHGSYTTFPPNGTG
                     PFTEQSTAAIVSMVNGTLQLYDPDTGMVVPSNLNQVSTMGPTSGRSALMADWSPDGNK
                     IVFASTPNPNQWIDLSGSSIAMMSYAYTGGQHTFGVPTFPFPNPITLQNGSYTNFFFP
                     SFSPDNALIVLNASRTSWRSNPARDAGQRLMLADAGGAWIVDMTEMNGGFADLDTTWA
                     HWAPTVGTDYYWIVFSTERDYGHRLTSANTDPSCVANGVTQCKQIWIGAVAKNKLSGT
                     VDPSAAPMWLPGQDMKADNISPYWSVPAGLF"
     gene            28033..28944
                     /locus_tag="VFV99_25375"
     CDS             28033..28944
                     /locus_tag="VFV99_25375"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5062739.1"
                     /translation="MTTRRDILRFAAVTSLLRGARVFAAEAKMTLNTRAIPKSGEQLP
                     VVGLGTWQTFDVGAGADERKAVREVLAKFLGAGMKVIDSSPMYRRAEEVTGDELAGIK
                     DASPFLATKVWTRGKQAGIEEMERSLRRMRTKTMDLMQVHNLLDVKTHLPVLREWKAA
                     GKIRYLGVTHYQHGEFDAIEKLMKTEKLDFIQIPFSIVDRLAEKRILPTAADTGTAVL
                     VMTPFESGELFTKVKGKPLPALAADLDCTSWAQFFLKFIIGHPAVTCPIPATAKASHV
                     IDNVQAARGRLPDAKQRDKMAATFVRT"
     gene            28951..29814
                     /locus_tag="VFV99_25380"
     CDS             28951..29814
                     /locus_tag="VFV99_25380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062740.1"
                     /translation="MAAVIVELAARKFTLPAECPCCGAPPDSELSVTAQATRQSLAFP
                     YCRRCTGHLQQWETSGVASAGLMVLAILAVIGLAVAGMFLLAIGVFVVGASLAWVLRS
                     SRRAAAKAACGESCATPGQAVTYLGWANATHRFSFASPTFAARFAEANQSLLANETPQ
                     LRKLLDGYRKARLAVPTPAVAAGVAPPPLTARDWTARLESTSGTVMRRIALLRALEMI
                     EEPQPRRELIQTVARIELAPLLDKLQRQSSPAAKRSLLNAAIAKIREDNIPDELQAAE
                     LEKLEARLAEL"
     gene            complement(29815..30570)
                     /locus_tag="VFV99_25385"
     CDS             complement(29815..30570)
                     /locus_tag="VFV99_25385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062741.1"
                     /translation="MKTVLATVATLFALTGANVAIAQPAAPAPAPQAPGNCCNTPTAQ
                     PASAPQPPPLLPVDEPQPVQQQPQVAVLAPDTSTSATEASGPSDDEGWKRPVGRRFMH
                     GFRLGWTYLFKMNDAQYKTRTDDNGMPVTIGEKYGLKSPNMMLLGYEGFYRIVGHSWL
                     NVLMVGNVTVAGLEQSKFIPAASGLIGFEFDRSFQLGVGVNLTPDPDAPSHMIAAAGW
                     TPSVGSIQTPVHFFFVPDTEGNHRMGMTVGMTW"
     gene            30710..31690
                     /locus_tag="VFV99_25390"
     CDS             30710..31690
                     /locus_tag="VFV99_25390"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX5062742.1"
                     /translation="MSATFLGRTCSHARAGAVVAILLATACRSQPAASRLFVSNESAG
                     TVSVIDAHSTKTLATIPVGDQPRGLRVSRDGRTLFVALRGAHSIGVVDLTQLRLVDTI
                     PSGRGPEAFDLVGDRFIVVSNEETQAATIVDVNARKVRATITVGNAPDGVAAAPDGSV
                     WITNEADNTVSVIDPRAARVVATLATGMRPRGIAFNHKLGVVTGESDASVTIVDIAAR
                     EAIARVQLPMTGVTPPQPVGVAIDAAGRYAYVTTGNAGSIAVIDLEAHRLARVIEHVG
                     HRPSAITLGRDGLLYTANGPGNDIAAIDPRSGLVVRRIAAGDSPGDITSL"
     gene            31700..31852
                     /locus_tag="VFV99_25395"
     CDS             31700..31852
                     /locus_tag="VFV99_25395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062743.1"
                     /translation="MIRKLSSGKYRLYSRKKDAKTGKRKNLGTFKSKADAEKHERAVQ
                     FFKRRG"
     gene            complement(31875..33815)
                     /locus_tag="VFV99_25400"
     CDS             complement(31875..33815)
                     /locus_tag="VFV99_25400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062744.1"
                     /translation="MEGDGRDSRDGDSDPSSRPRVTRLYGAIDLEASHPSTLVDGDKT
                     TIGAPPVATPDIGSMLERYRIGDVLGQGGMGEVLSARDEQIGRQVAIKRLRVENPTPE
                     IMARFLREARIQGRLEHPAVVPVHELWNEGGQPFFVMKQLTGTTLADVIPKLAGRDQR
                     AADEFTLQRLLRAFADVCLAIEFAHARRVVHRDLKPANIVLGDYGEVYVLDWGIARIT
                     EDPNEPKRDSFDDIGTMADIRPMADITTTAGTETVVGAILGTPGYISPEQIRGDHFLD
                     GRADVYSLGCILFEILALAPLHPRGQPGIASALAGVDARASVRAPNREIPPELDQICV
                     KATQVDPQLRYDTARELGTAVQRFLDGNRDVALRRELAKAEMATARKALGKGDGPEER
                     RDAIRAAARALALDPTDREPADLVGALMLEAPTVMPPEVEEELAAIDEDALKISARFG
                     MLAAIAYLGFFPVLYYIGFRDAWYNIVGPLICFTIILVQALIAPRNPYVSGYITITLN
                     LAMFALFGWMVSPIVIGPGPAIIMVSLMATHRKLIRPWLLALLTVIATVSPWLLAIAG
                     VLPDRVSVSGNTLLLQTPAGELDPNATLVGLFIYIVALIHLAALLSRLQDDDRRKVRR
                     TMQLQSWQLRQLVPRPSSKPPR"
     gene            33922..34053
                     /locus_tag="VFV99_25405"
     CDS             33922..34053
                     /locus_tag="VFV99_25405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062745.1"
                     /translation="MSIAPSPRSPQSPADDMLPGMRLLLLVLALVGVDLRVVYAGGA"
     gene            34105..>34485
                     /locus_tag="VFV99_25410"
     CDS             34105..>34485
                     /locus_tag="VFV99_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062746.1"
                     /translation="MPPGQFMLRLGWLGTYVQTQHPDFADVVGQGTIPIIHDQDIAIT
                     EARLSLDAGLTKRFSASLVVPFRVVSTSIVYRNGMGMPVELADPNIHHRNETLTGIAD
                     PIVLGAFTYALGDVRFTARGGLSIP"
BASE COUNT         5766 a        11227 c        11529 g         5963 t
ORIGIN      
        1 tcccggtgcc gacgaggccg cgttcgacga ggatctgacg ttcacgccgc ctcgcacgaa
       61 cacggcgacg caatcgatgc cgccgccgac acgtacgccc gcgccgatcg gcgtcgtgaa
      121 gtcgggcggc acgtcaccgg gcacaccagc gccgacgtca cggacgccag acctggcgac
      181 gccaccgttc gcgacaccga tcgcgcgctc gggggtgggc acgggaccgc ggcctgtcgt
      241 gtcgccgcca acaacgatac cgtcgatcgc gacgcgtcct gcgggtacat cgacgccact
      301 cgcgacgccg ctgtccgcgc ggtcgacggt cgcaaccccg ccgccgagcc cggtcgtacc
      361 gcggccggca tcgccgacgc cgagcacgaa tcccgcgttg ccgcgacaga ccgcgaacac
      421 gccggtcacg aatccgacgg tgccacgcac cgaggtgacg ccattgccga cgccgccggt
      481 gagtgcggca gaatcgccag agccaagtgg ctcgctggat atttcgccat cgatgatgct
      541 cgacgtcgag ccgctgcgtc gcgccccgac ggcgccgcca ccaccgccag tgaagcgtcc
      601 gccgacggcg ccgcctgctt ccgtcgaagc cgatcccgaa ccgtcgagcg acgcgccgac
      661 tcgaccaacg ccgcaaccga atgccgcgga gctgtgggcg cgctcgatgg cgccggcaga
      721 gtcgccgaaa cctacgctcg ccgattcgac cgagtcgacg atggcgaacc tgttcgcgac
      781 ccagaccgtg cgcaagcgtc caccgacgcc accgcccgcg cacctcgaga cgctcggcag
      841 cccgttcgcg cccggccagc taccggaagg cgacttttcg tatccgatca agcgcagcgg
      901 ctttggcgtg ctgctcaaga tcgtgctcgg cctcggcgtg ctcgcgctcg gattcttcgc
      961 gttcgtgaag ttctatccga tggacgacaa cacgccgacg cagaccgcgg cggtgactcc
     1021 accggcacca ccggagcccg caccggcgcc ggtcgtcgag gctccggccg ttgttgtcga
     1081 tgcgccgccg gcgacgccgc ccgcgccgat cgagaccgag cagccgccaa cgccaaccga
     1141 gacgccagcg ccgccgactc cgccgacgcc gaccgaaacc gcaaagccgc cgacgaaacc
     1201 gaccaccaaa ccggcaacgc cgacgacgac gaagccggcg acgaagccga cgacggagac
     1261 tggctcgagc ccgacgacag aatcgacgcc aacaccgacg cataccgaaa ctgcggacac
     1321 caagccggag tcgtctggcg gtgactgcga tgaagttggc tgcgtgatct cgaaatacga
     1381 tcgcccgtgc tgcgcgaagt acaagccgag cgacacgttc acgccgaaga acgtcgtgcc
     1441 ggacgagctc gatcgcgcga tggtcaaagc aggtgtcgag aagatcaaac cgcgcgtcgt
     1501 cgcgtgtggc gagaagaaca gcaacaaagg cacggtgcgt ctcgcagtga cggtcgacag
     1561 cgaaggccac gtgaagaccg tcaccgtcag cgaaacgccc gacaccgcgc tcggtgaatg
     1621 cgtcgctgcg gcgatgcgcg ctgcgacgtt cggcaagagc gtcaacggcg gcgagttcaa
     1681 ctatccgttc gtgttctagc gtcgcctcga ggagacagtc tcctcgagga cgcaacgctc
     1741 ggggggcaat ctcccagtcg gcaacaaact cgacttgcca gtcgctgcgc gcatattcgc
     1801 gcacttgccg tggcactcgc gctgcaaatg cggtcgcgca tgcaaacgct caacatcctc
     1861 gacctcgaag ttgtcaccgg tggcggtgac gtgcgtgctc cgctcagcgg cgacgtcact
     1921 caacagatca acacgaactg gggcggcacc cagatcatca acccgccgcc cgcgccgccg
     1981 aagaagccgt cgggcctgcg catcgattat ctgcgcgagc acccggaagc gcggtgggga
     2041 gcgccgccgt acgtgaaccg gcccgtgcgt cgctagctcg ttccaacacg tgcacgtgtc
     2101 acgtgcacgt gcacgtgcac gtacacgagc aaacgtacat cccgctcaac gttggatgac
     2161 acttcgttat caacccgcgg ggcacagcca gcgctcgctc tgggatggca ccgagcaagc
     2221 ggacgactcg atgatcacat ccgacttcgt cgcctcgccg caagcaatcc gcgcgtcgta
     2281 cacgagcaaa tgtacatccc gctcgacgtt ggatgacact tcgttagcaa cccgccgggg
     2341 cacagccagc gctcgctctg ggatggcacc gagcaagcgg acgactcgat catcacacgc
     2401 gacttcgtcg cctcgccgca agcaatccgc gcgtcggttg ttctgatcgc tatcgttcgg
     2461 cgtcgaggac tgattcgcgg cggttgcccg ctcgtgcacg tgtacgaaag cgtcgctagt
     2521 tcttggtaat cgtcgcggtc aacgtgccgc agctttgacc ggcatcctgg tgcgcgacga
     2581 gccagaacgg tcctggtttc gtttgcggat acgtgattcc ggtgacgcgg tcggcacatc
     2641 cgtacggctc accgagtgga agaaacactt cgagatcgcc gacccatgcc atgccagccg
     2701 tcgccttcca cgtgccgcta cagcccatga agaaccacga tgtgtcctcg acgtccgatt
     2761 gcacgatgca accggtcgat gggtaccagt cgttgtggcc gcacgtcgtc gcgctgttcg
     2821 caccgatccg gcccgcgatc gatggtggac cgatgatcgc gcgtgcggtc gcaacgtgtg
     2881 atgcgccggc cgctggctga tcgatcacga cgcaatacgc gccggggttg agaatctcgc
     2941 tccactgctt cgtgcgcgca tcgcacgaac cggcgccact cgcgatacac gcgagctccg
     3001 tcttcgcact cgacgcacac ggtccatcgt agatggcaag cacgacgtcg tagtcggtct
     3061 gcgacgtgtc gacgtagagc cgggtcaacc cactcggcac gctgacgtcg aacatgacat
     3121 caacgccgcc gacgcccgcg cattgtggtt tgacatcatc gtgcgcgttg ctcgcatcga
     3181 cgaccgtcgg cgcgccgtcg ttggtgagcg tgtagatcat gtccggacct gcgttgccgc
     3241 tgccgctgcc ggcgccccca ccggtgcaat caccggcgcg gtcgccggcg gagtcgccat
     3301 agcgatagcc tgactggcat gcggtgtcct ggtcggcgca gaagccgtcg atgcacaagc
     3361 cgccgctgca cgtcgggttg tccgcgcacg gcagagccgt cggctcgccg caaccgacga
     3421 gcgccgccat catgatgaag gcgccaaccc tcaccagaca cctccgagtg tgacgaccgc
     3481 accattgccg cgtgcgggag tgactgtgac gctgcgttcg tgaagtgaca gacgcaacac
     3541 gccgagcacg acgcaggtcg cgcccgcacc gagtgcgacg ccggcaacga ttcgatcacg
     3601 ctcccagcga tcgcgtgcga ccgtccactg atcgacattc gtcgcgagcg ccgtgtcgct
     3661 ggcggctgca cgcgcggcga cggcgaagcc ggcaccgatg ccgagtccga tcacgcccgt
     3721 cgtcagtaac gacataccta cgctgtcgcg ccaccaacgt gaggtttcgg tatgcgatgg
     3781 cggcggcggt tctcgagctg gctcgggttc tggttccgct gctttctgcg gttcctcgcg
     3841 cgcgggtgca ggggcgacct ccacgggcgg cggcaacgtc gtctggcagt tctcgaggtt
     3901 tttcttcgcg cgcgtggctt cgttcgcggg cggctcggtc gcgaggaact gcttataggt
     3961 gtcgatcgca gacttgcaat cgcccgcgag ccgctgcgcc tgtgctttgt cgtacaggaa
     4021 gatcggttgt ggatcgatcg cgtagccggc atcgaatgct ttgatcgctg cggcgtagtc
     4081 ctttgcgcgg aagctcgcct caccgcgctc gagctcggcg ttcgcagcat cgctgagctt
     4141 gcgatccgca atcgcagtgc cggtggcgcc ggcgaccagc aagacactaa tgaggaagca
     4201 gcgcatccag atcgtcctct gcggcttgcg gggggggagg tgccggtacc gcgcgcgtcg
     4261 gctgcacggt tcgtaccggc ttcttcttcg gtgtcggctt cgcgcttggt cgcttccttg
     4321 caggagaagg cgaggcgtgc gcgacggcgg tactgatctc gatgctgggc tcgcttgcgg
     4381 ctggtttggt cgcctcgggt tcggattcga tcggaatgac gctgatcatc ggttgggcaa
     4441 ccggttcgtc gactaccgcc gtcgcgcgcg gctgttctgc cggctgtggc gcaggtgagc
     4501 tgttcgcgtc gagcgagcgc cacgcgacca tgctgaggat cacgacgagc ggtgcggcga
     4561 gaccgagcgc gatccgcgtg cgccacagca tgaacgggga gtgcttctcg cgcggcacgg
     4621 tcgtcgcctc gccaaactgc agcgactcga acgccatcgg cgtggggcgc gacgtgtcgt
     4681 accttggtgt cgacgtcgtc gccttcactt cgagcacggg cgctggatct ggtggcgctg
     4741 gcaccgcggg cgcggggaat cgcttcgtcg tcggcgtgcg ctcggtgatc tcggttggcc
     4801 cggcgaacgc tgagggcttg ttcgcggacg ggcggagcaa tcgtgtcggt gcggtgatcg
     4861 gtcgcgacgc gaagtccgac gtgccgaccg gatgcgtgtt gtcgaacgac tcgaacggct
     4921 cggtcggatc gccggggaga gcgacgatgt cgtgcgcacc gctcgtcttg aacgcggcga
     4981 cgtgctcggc gagcgtcttg ccgagccgct gcgcttggcg ccacgcttcg aggttctcgg
     5041 cgaacacgcg ctccatgagc gccgccatgc ggaacggcga cagatccaac ccgcgattgc
     5101 gcgcgaactc gtcgagcgcg acccgcattt cctcggcggt cgagtagcgc tgcgctggat
     5161 cgcgcgcgag accacgcagc acgatcgctt cgagctcgag cggatagccg cgaacatgcg
     5221 acgacggtgg cggcggatct tccttcgtca ccttcacgag ctcctcgaac gccgtcgaga
     5281 ttccatacgg tgttcgtccg gtcgtcagct cgtagagcaa caccgctgcg ccccacacgt
     5341 cggtgcggcg atcggttttg cgacccttga tctgctcggg cgcgatgtag ccgggtttgc
     5401 ctttgatgaa gccgtgatcg gtgtggtcct tgcgcatcga cgcgcgcgcg atgccgaagt
     5461 cgacgagttt caccgcgccg tcgtaggtaa cgaacacgtt atgcggtgac acgtcgcgat
     5521 gcacgatggc gagcgacttg ccctcgctat ccatgcgttc gtgcgcgtga tgcaacccag
     5581 cgcagacacc ctgcgtgatc gcaactgctt gatccagcgg tacatgctcg ttggcccgct
     5641 tcatgcgacg gagcagatgc gagacgtcat gaccatgaag aaactccatc acgatcgaaa
     5701 cctgtccgtc ggcgatttcg atgtcgttga cctgcacgat gttcgcgtgc gagagcgtcg
     5761 ctgcgatgcg tgcctcgtcg aagagcgcct ggacggccga tgcttgcgat gcgagtcgct
     5821 tcgcaattcg cttgatcacg acgagacgct cgaagccacc gggcccgagc gttcgcgcga
     5881 gatagaggtc ggccattccg ccaccggcga gccgacacac gatctcgtag cgtcggctca
     5941 caccatcaac catgtccgac attatgcgcg gtatgatcgg aacgtgtcga cacacgttat
     6001 tggcgtccgc cccgatgcct tcacgtatcg acgactccgc gtgcgcgtga tcgacggacc
     6061 ggaccgcggc acggtggtcc acgacgctga cggagagatt gctattggta ctgccgatgg
     6121 caacacgttg gtgctcaccg atcccactgt ctcccgctat cacgttctga tcacgagcag
     6181 cgacaagggc gctcacctca gggatgttgg ctccacgaac ggagttttcc tcggggagca
     6241 ccgcgtcgaa tcggggtgga tcgacacggg tgcggtcctc aaactcggac tcaccactct
     6301 tcgattcgag tacggggaag acgacgccga gcagccgctt tccgccgatg attatttcgg
     6361 ccgggcgatt gggcgctcga tcgcgatgcg ccgcgtgttc gaggctctgc cgcgcattgc
     6421 cagctccgac gcgaccgttt tgatccaagg cgagaccgga accggcaaga cgttgctcgc
     6481 tgaagccatt catcaagccg gaccgcgcgc gcgcgctccg ttcatcgtga tcgactgcgg
     6541 tgcgattccg ccgacgctga tcgagagcga gctcttcggg cacgagaagg gcgcgttcac
     6601 tggcgctgct ggtgctcgtg tcggcgcgat cgagagcgct gctggtggca ccgtgttctt
     6661 cgacgagatc ggtgagcttc cgctcgacct tcagccgcgg ctcctgcgcg tgatcgagga
     6721 ccgcatgatc aagcgcgtcg gcggacgcga tcagattcgc atcgacgtcc gtgtcgtcgc
     6781 cgcaactcat caggacctgc gggctgcggt caaccgcggt gatttccgct ccgacctcta
     6841 ctaccgactc aacgtcgtct cgctccgtct cccgcccctg cgcgagcgac gcgacgatat
     6901 tccactgctg gtcgagtcct tctacgaaca gttcgccggg catgcgggcg caccgcaaga
     6961 cttgatcgac gagctcgggc gccatgattg gccgggtaac gtccgcgagc tgcgcgcggc
     7021 ggtcgagcgc gctgttcttc tcggggatcc tgctctgtgg caggcgataa cggacgagac
     7081 gcgcggcgac gtcgttccag ccgttgcgaa cgagccattc gacgactcgt tgtcctttcg
     7141 tgcggcgaag gaacgagcgc tcgtacgatg ggagcgcgga tatattgctg agctcctgcg
     7201 tcggcacaac gggaacctct cgcgagcggc gcgtgctgct cgcatggacc ggacgcacct
     7261 gcgcgagctt ggccggcgct atcatttgct gccttccgtc accgacgacg aatgactcgc
     7321 agtcacggcc tcgcacggta cccacgtact gagggcgggc gccgccacca cctgagggcg
     7381 cgcgccgtca ggtaccaggt acagctgccg agctagccgc ggaggcggta acggcgacgg
     7441 agacccccga ggttcgacgg catacatcgg cgagtcgatg gtgcaaagcg agtaccggca
     7501 ccgctcttgc tctgcgacaa ggcgatgagg tgggtcagtc ccgagcactt gcgagttacc
     7561 cacggtgaaa ttcgcgaggg ccggatcgtg agtcgcgcga tgcgcgcggt cgctctcgcg
     7621 agtcgcggcg acgctgctag cctcgtgttt acgctgcacg gaatcgtgtt cgacccgacg
     7681 ccaaagctcg gggtcgagct gcgcgccgct aacacgtcgc atctcgtgta cgtgatgtgg
     7741 cggctgattc cgaagacgcg gctgaccgtg caggtgaaag cgccagatgc ctcgtacttg
     7801 cgagtcaaac cgattcactc cgagctcctc gacgtgccgg agctcggtcg cgcgcagaaa
     7861 ctagcggcac gcatcgaagg cgatcggctc agcgcgtgga tcgaaaatca cctcgtgtgg
     7921 atgggcgtgc tgccaccacc ggcgcgtgcc ctcgttggat ttgccggcat gcgtgccgat
     7981 cacgtggcgg cggacgtcgc gcttgccgcg gattgatatg gctcgcgagt tgctgggctt
     8041 gttcgcatca cccagggaga ctgcaaatga agtgctgctt gctgatcttc gtgtgcatcg
     8101 cgtgcggacc gtcgaaccat cacggctccg attgcactgg cgcagattgc agcacggggt
     8161 cgtgtaaccc gggcgactcg cgcgactgct acacaggaga agccgtcacc aaggacgtcg
     8221 gtccgtgtca cggcggcaac cagctgtgca caggcgcagg gcagtggggc gagtgcatgg
     8281 gccaggtgat ccccgccggt gagtcgtgca ccgacggcct cgacaacaac tgcaacggca
     8341 tggtcgacga gatgaccgat ctcgatggcg acggcttcgc gacctgcgat tcgagtggcg
     8401 catcgctcga ctgctgcgac tcggtcgagt gcggcacacc ggctgcggtg aatccgggcg
     8461 cgtacgatgt tgcgggcgat agcgtcgaca acgactgcga cggcacgatc gacaacgagg
     8521 tcggtctttg cgatcagagt ctcgcgtcga actcgcaggc ggcgagcgat tacgcgaagg
     8581 ccatcgacat ctgccagaac gcgacgccga cggacaagaa gtggggcctg atcgaagcga
     8641 agctgaccct gccaagcggc acgggcgtgc ccgatccgca gtcgtattcc atccggcaca
     8701 agttcggcag caaggtgctt ccgcaaggtg gcagcagcgt cgcggtgatc tcgaccggcg
     8761 gcgcggcggc gaaaggcgat atcaatccgc cgtatcacga cttcgtcagc tacgagacga
     8821 cgaagacatc gccgttcccg gccgacttcc tcgcggcgaa cggcggcagc ttgccgaacg
     8881 cgccgggctg tcccgaacca gttggcgccg tcgcgaacga tccggtgatg ctcacgttca
     8941 gaatccgtgt accgacgaac gcgcgctcgt tctcgctgcg cagcaacttc tacagcagcg
     9001 agttccccga gtggacgtgc tcgccataca acgacttcta cgtgatcctg atcgactcga
     9061 cgtcgacgga caatccgacc gacaagaacc tcgcgttcta ctcgccaccg aacgcgatgg
     9121 cgaaggtacc ggtgggcgtg aacctcggct tcggcaacac ggggctgttc acgcaatgtg
     9181 tgaacggtgc gaccggctgc gcgaccatgg gaatggggca ggccggcacg atctcgacgt
     9241 gcgcgggcac cgacgatctg atggcgaccg gcttcgatga cccggcgccg atggaatgcg
     9301 aagcagcaag cctcaaaggc ggagcgaccg gctggctaac gacgagtggc aacgtcgtcc
     9361 ccggcgagat catgacgctc cgtatcgcga tctgggacac cagtgaccac gcgctcgact
     9421 cactcgccgt cgtcgacggc ttcaagtggt cggccgatcc gtcgacacct ggcacggtga
     9481 ttctcaagac gcactaacag agtccgagtc cgagtccgag acacgcgtac tgacagtccc
     9541 agtcccctca tccctacggg ttggtacgtg catgcctcgg actcggctca ggacacttag
     9601 aggtgtccac gtcatggttc atgtgatgag cacgacgctt catcgcctgt gtgtcgcggc
     9661 cgaacaggcc gccacgcgcg gcgatcacca cagcgcggtt cgcaactaca tcgcggcggg
     9721 cgacgctgcg cggaaccgag agcgctggca actcgcagcg aggtgctatc gcagtgcgct
     9781 cgagatcgat ctcggatcgc gagtagctgt gtcgcggctc gtgcacatcg gtggtcacac
     9841 gaacgaccgg cgattctggg tcgagtattc gcggatgctc aagcacgacg cgtcgtggtc
     9901 gcagctcacg tgccggcgcg cacagatcct cgtccataac ctgggctccg tcgtcgagtg
     9961 cgccgaggtc ggtcccgtga tcgagctcgt gatcgcgtcc gatggcagct tcttatgtct
    10021 tcccgacgcg cggttcagag caatgccact cgcgatgacg ctgatcatca tgcgccgtgc
    10081 gctatggccc acggggcgca gctcgagagc cgcaccacgc gttcgcctcg gctatcgcgg
    10141 ccacacggtg tggctgaatg cgcgcggtga ctggtcgctc ggcgattagt gcggctggac
    10201 cttcgtcaga tctgtattcg cgtagttcgc gccggctttg acctcgacgg cggtcgagta
    10261 cttgatcggt tcgctgctgt tcgaatcgtt gcgcacgatc gagagctcgt agcttcccgg
    10321 ctcgagtgtc tgccgcttga agtaacccga cgcgtcggtc tccgctaacg cgtacggccg
    10381 atcgctcgac gtgccgttgc ggagctggat catggcgcca ctgatcggat cgtcgccgag
    10441 cgtgatggcc ccttcgagcg tcgcagtacc cgcgaaatga agcacgatgt cgctgctcgg
    10501 ccccttggcc ggcacggtga tgcgtgcgac gccaccgcgg cgcccgtacg cttcgacttc
    10561 gagcgcttga tccgccggag ccacgaccga gaacgttcca gtcgccgagg tcgtcacgcg
    10621 aagctcggag ctgccttcgg cgtgcacgag tatatcagcg tcggcgatcg gttcttgctt
    10681 tgcacgcgac ggtcgtcgcg cacttcgcaa cacccgagcg cgcggcgtcg gcagcgaaac
    10741 agacgttcgg cggcctcgta gcgaatctac gcagcacggt gcatgatcgc gacgtgacgt
    10801 tcacggtcga gctcgatgcc acgcaaaccg cgcaggtgat cgatcaattg actgctctga
    10861 tgaggtagcg atggcctggg tcaagattcc cgcggagcac catgcgctcc tgatcgcagc
    10921 gttgccgaag gatccgcgcg tgcgcacgat caacatgttc ggcggcaccg cggcgctcgt
    10981 gaacggcaac atgtttggcg gcacgttcgc gcgctcggta atcgtcaagc tcggtcccga
    11041 cgactatccc gaagcgctcg cactcgacgg tgccgagccg ttcgatccga tgggcagggg
    11101 ccacgtgatg tcgaacacgg tgctccttcc cgagacggtg atggacgaga ccgccgagct
    11161 gaagagctgg ctcgcgcgcg cgttcacgta caccgcgacg ctgccgccaa agaagaaggg
    11221 cgccgccaag aagcccgccg cgaaaaaagt tcccgcaaag aagctgggcg caaagaagat
    11281 ttctgccagg aaaccggccg cgaagcgcaa gaagcggtcc tgagcgagtg caacaggtca
    11341 gctagcggcg gtgtcgcagt ggtatggaca gcgacgtcgt cgaagtggtc gcccgcttcg
    11401 gcgggacgat tctcgacgtc gcacacgtag ggccggccga cacgtatcgg atcggtacgg
    11461 cgcaaggcac gaacctcagc gtgcctgggc tcacgtgctt tccgctcgtc accggcggca
    11521 aggtgcgcca tcccgtcggt gttccggtcg tcgagcgcga cggctggacc gagatccgcg
    11581 tcggcgcgct ctcgatccac atcacgcgta cgaagctggc gcgtgcgcca ctcgcgcgcc
    11641 cgcgcatcca gtggcgcacg ccggcctttc ttgcggcgtc actggtcgtc cacatcgcga
    11701 tctggttcgt cgcgctactg catgcgccga tcgagaagct gccggcacag aagccgcggc
    11761 cgcgcctcgt gcacgtcgag gtggcgccgc cggagccacc gccaaagccg gcaccgcacg
    11821 aggccaccga gaacgaacgc aagaaggcgt cagtagcggc cgtgcaaccg agccggcgac
    11881 cgcgtgtccc gcgcgaagag cgcgacgagc ggattaccac gacagaagcg gctgtcgcgg
    11941 cgatggcgcg ctcgttcgac gcgatcgacc tcgcgggcaa gatggcggac ctgcgtcccg
    12001 aggacacgta tcgcgaggat gaagcaaacg cgcgcggttt cggcggtggc ggtaccgggc
    12061 gccgtttcga tccaggcccc gctgaatcga tcgcgagcgg cccctacgcg acgatgatct
    12121 tcgacgtgaa gctgtgcccg aacaaagcgt gcaccgtcga aggcccggtt cccgcgttgt
    12181 tcgtgcgcac gcacctgctc gcgcacatgg acgagatcta cgcgtgctac atggagcatg
    12241 cgagcgagcc gggaacgatc gtgctgtcgt tcacgatcac gcccgacggt gcggtgcgcg
    12301 acgcgcgtgg taccggcctc ggcgagaccg gcccgtgtgc ggcgcgtgtc gtcggcgaga
    12361 tcttcttcaa ggcactcgga aacgactacg tgccgccgcg ctcgacgtac gtgcggcgct
    12421 acccggtgcg gttcaatcca gcacgcggct agcgcgtcag ttgatcgaga tgcatgcgga
    12481 tatgcgcggc ctcggcggcc gtgttcgcga gcgagattgc ctgcgtgaac gcggcacgcg
    12541 cctcgcgatc gcggccgagt tgctgcagca gtgcccctct gaggccatga aagtgaaagt
    12601 agcccgcgag cttggtctcg agtggctcga tcatcgcgag tgcggccgcg gcgcccttcg
    12661 tcttcgacac cgcgaccgcg cgattgagcg tgacgacggg tgacggctgc agtcgctcga
    12721 ggttcgcata gagaagatcg atctgcgccc agtcggtgtc ggccggcgtc gcggcgcgtg
    12781 agtgaagtgc ggcgacggcg gcctggatct gataaggccc cggctgcgcg tggcgcagcg
    12841 ctttgtcgag catcgcgagg ccctcggcga tcaggttgcg attccacttc gtgcggtctt
    12901 gcacgtcgag cagaacgacg gcgccgtcgt cgtcgaagcg cgccgcagca cgcgcgtgct
    12961 ggagcagcat cagcgcagtg agccccatga tctcgggctc gctcgcgaac agccggatca
    13021 acagccggcc gagccggatc gcttcgtcgc atagctgcgc gcggtcagcg tcctcgcggc
    13081 ccgcggaata cccctcgttg aaaatgaggt agatcatcgc tgcgacggcg ctcatgcgtt
    13141 cggcacgctc gtgcgcgtcg ggcgtctcga atggcacatc ggcggcggcg atacgattct
    13201 tcgcacgcgt gatccgttgc tccatcgcgg cctcggtaac gaggaacgcg cgcgcgatct
    13261 gcttcaccgt cagtccggat acgatgcgca gcgcgactgc gatctgctgc gacggcggca
    13321 aatcgggatg acagcagatg aacagcagcc gcaacacgtc gtcgcggtag tgcgcgtcgt
    13381 cgaggcgctc cgcgagcgag ctctcgcttt cggcggcgac atcatcgtcg gggaggtcgg
    13441 tctgcttctt cttgcggcgc gtctgatcga tcgcgatgtt gcggccgacc aggatcagcc
    13501 atgcggtcgg attgcgtggc ggaccgttct cgggccacga cttgagcgcg cggagcgatg
    13561 cgtcttgaaa tgcttcctcg gcgaggtcga cgtcgcggaa atagcgaacg agggcgccga
    13621 gcgcctgggg gcgggccgag atcagcgccg cgtcgatcca ggcaaggtca gtcatgcgat
    13681 gggcccgccc ggctggtaga gcatcagcgg ccggatctcg aacgcaccgg cagagccgct
    13741 cgctgctgcg agctctttcg catcggcgag tgcggcctcg aggctcgccg cttcgatcac
    13801 gtaaaatccg agcagctgct ctttggtctc ggcgaacgga ccgtccatca ccatcgcgtc
    13861 cttgcccttg cggagggtcg ttgctgcggt cgtcggcatc aggcgcgcga ccgggccgag
    13921 ccgtttctgc tcgccgatct tggagatcac cttgccgagc gacgccatga cctcggcatc
    13981 tttctccttc gaccaggcgc aggtgacggc ttcggagtcg tagcaaagga tggcgtagag
    14041 catcgggtct ccttccaagg acgaacgagt atgcctccgg ccgacaacac tcggaagtgc
    14101 ccgaaatccg agtgcacgcc ggcggtcact cggtgttacg ctgagccttc gatggcgtcc
    14161 aagctcgagc aaagcgagct ctacggactg aaggcgttgc ggggagtgag tctggattcg
    14221 gtcgaggagc tgctaggaac ctgtgaactg cgccggctgg cgccgggaga cgtgttgctc
    14281 acgctcgggc agccgaacga cacgatgtac atgatcttgt cgggcgagct cagcattcac
    14341 ctcgaagggc cgacaagcga agcggtcgcc acgctcgggc ctggcgaaac cgtcggcgag
    14401 ctgtcggtga tcgatcagtc gccagcgtcg gcgtacgtcg tcgctgccga gccgacgcga
    14461 ctgctcgcga tcgacgaacc gcgattctgg aacctcgtca acgcgtcgca cgacttcgcg
    14521 atcaacttgc tgctgtcgct cgcgaagcgc ctacgtgcga acaactcgac ggtgtcgacg
    14581 aacatcaagc tgcagcgcga gtacaagcgc aacgcgctga tcgacggcct cactggcctc
    14641 tacaaccggc ggtggatcga cgaggcactg ccgcgcttcg tgcagcgtta tggtcgcggt
    14701 ggccagccgc tcaccgtgct gatgatcgac gtcgatcact tcaagcggtt caacgacacg
    14761 tacggccacg cgatgggcga tcaggtgctc gtgcgcgttg gccaggcgct gcgcgcgagc
    14821 ttgcggccga ccgatcacgt cgcgcgctac ggcggcgaag agttcctcgt gatccttccc
    14881 gacaccgtcg gggacgcggc ggagatcgtc gcgaatcggc tgcgcacagc ggtgagcgac
    14941 atcgacctcg tgcaggcaga tggcacggcg attcctcgca tcacgatctc gctcggcggc
    15001 ggctcgttgc gctcggggca gacgatgaag gcattgctcg gacacgccga cgaagcgctg
    15061 tatcagtcga aggcgaacgg tcgggaccgc gtcacgtttg cggtctcgtc gtcatgacgg
    15121 acgccatcga tccggtaaag cgcgcctcct tcgacgggat ggctgcgcgc tacgacgcgg
    15181 cgcggccgtc gtatccgagc gccgtcgtcg acgacgtgct cgcacgctct ggcgcgaagc
    15241 gggcgctcga ggtcggcgcc ggcaccggca aggcgaccgt gctattcggg cagagtggcc
    15301 tcgacattgt tgcgctcgag cccggtggtc agctcgctgc gctgttacgc gaacgggtcc
    15361 gcgggatgcg cgtcgaggtc gtcgagtcga tgttcgaggc gtgtgacctg accggcttcg
    15421 acctcgtgta cgccgcgacc gcgttccact gggtcgagcc gagcgtgcgc tacgtgcgag
    15481 cggccgaggc gctgcggccg ggcggggcgc tcgcgctgat catgaacgag aaggcgccaa
    15541 tggatcccac tacacgcgac gacttcaacg cagcctacgc gaagcatttt gggtggccgc
    15601 agtggaatcc ccagtacctc gagcgcaccg aggcatcgtg ggtcgacgag atcaacgcga
    15661 gcggacgatt cgggccggtg cacgtcggcc ggtttccgtg gtcggcgacc tacacgaccg
    15721 acgagtttgt cgcgctgctc gatacgtatt ccgatcacgt ggtccagccc gacgacaagc
    15781 gcttgccgct ctacgacgac atccgagctg cgatcgatcg gcgcggcggc agcgtcgaga
    15841 ttccgtatct gacgttcgag ttttttgcgc tacggttgtg agatgcgcgt cgtcgtctac
    15901 gacaagacct gtgtgaagac gcggggacgg ttgtcgccga tctgggcaac cggtgcgcgg
    15961 ctctatcgcg cgttgcgccg cgccgacgca gtccacgggg tcgcgagctg gggcgaggcg
    16021 ctggcctggc tcgcctcgca caagcagccg ctccgcgaga tccagtactg gggacacggc
    16081 aagtggggac gcgcgctcgt cggcgaagaa tcgctcgatg caacggcgct gacgacgcgc
    16141 cgcgccgagc tcgaggccgt gcgcgagcga ctggcacccg attcactgat ctggtttcgt
    16201 acctgcgaga cgctcggcgc gaagcccggc atcgacttcg ccgaacggct cgccgatttc
    16261 ttcggcgccc gcatcgccgg tcacacgtac atcatcgcgt ttcatcagag cggtctgcgc
    16321 ggagtcgcgc cgggctgccg cgccgactgg tcaccggacg agggcgtcgc ggcaggcacg
    16381 cccgaggcgc ccgaggcggc gcactggtcg cgaccgtggc ggccacgaac ggtgaccgca
    16441 ctgcacggcg ccgttcccgc ggcctggatc acgcgctgac gaacgcgctc gcgttaggat
    16501 gggtgtgtgg ttcgccgcac gctcgtgctg atcgtcgtca ctgcatgcgg tagcgagcac
    16561 gatccggtga tgcccgacgc ggcgggcaat ccatcgatcg cggggacgcc ggtgagctcg
    16621 ttcgagacgt cgtcgtgctc gacgggcgac gtactcgcgc tgtcgatcca gatcgccgac
    16681 gaggtccact gcatcatgcc gggccagctc gtcgcgttcg aagaaggacc cgcgatcatg
    16741 ttcactggat cggccgtgct gccgtacgtg agtagcgcgg cgcgcgatgc gctctacgcg
    16801 gcgaccagcg gcagcggcaa cgtgttgcaa gtgacgagcg ggtttcgcac cgtcgtgcag
    16861 cagtacctct tgtaccgctg gtttcagatg gggcgctgcg gcatcaccgc ggccgccgac
    16921 ccggggacgt ccaaccacga gagcggacgc gcgctcgacg tctcgaacta caacacgtgg
    16981 atcacggcac tcgaggcgaa cggctgggcg catgacgtgc ccggcgatcc ggtgcatttc
    17041 gatcacctct cgtcaccgga cattcgcggc gccgacgtgc tcgcgttcca gcggctatgg
    17101 aaccgcaacg cgcccgacga tccgatcacc gaggatggtc agtacgggcc gatgaccgag
    17161 gagcgcgtta agcgtgcgcc cgcggaaggc tttggcatcg gcgcgccatg tgcgcagcgg
    17221 ctcgcgccgc cgatcgcacg gagcggggaa gggaacgacg aggtcgaatg cgcgcgttga
    17281 tcggtaccta ttgctcgcgc aggaccaaca ggccgcgctc gagatccgca acgtagagga
    17341 ggtcgccgat cttgcgcacc gcgagcgcac cctcgaacgc accgccgtac gcgttgtctt
    17401 cgatccagtc gttgtagtgc gcgacctcga cgggatgcgt cgggtcggtg agatcgatga
    17461 cgcgcacgcc gtcgtggtag taggcgatgt agaccttgtt gccgtggacc tcgaagtagt
    17521 gaatgcccac ttcgcgccgc gtctggtaga tcgcgagctc gtgcatgaac gtcggtgacg
    17581 ccgtatcgcc gtcgaggatg ctgagatgcg cgccgccgct cggcgacgcc gtcatacctt
    17641 cgtcgcccgc cagcacgatc ttcctgccgc cgatcgtgcc tgcccacgct gcgtggctgt
    17701 agccgagcct gtacctgccg agctcgacgt tgctggtgcc gaagccgcca ctcgtgtcga
    17761 agccgacgag gccgagcgtc gtgttgcacg cgtagatctt gtcgccgtcg acatagagat
    17821 cgtgcacgcc gctgacgtcg cccggcacga tcgccatgcc ctgacgcacc ggtgtcagtg
    17881 gtgcggtcac gtcgtatttc ggcaccgatt cgttgtacgt cgcgaggtac agctccttct
    17941 tgccgttgcg ttcctggatc tggagcgagt gcgagtactc ctggagcatg ctgacgaacg
    18001 tcggcgccgc cgggtccgtg acgttgacga cctgggtcgg atcttccgac ccgaacggcg
    18061 acaggaacgc gacgacgttc gtgccgtcgt tgacgacgcg gacgtcgttc caggagccgt
    18121 cgtagtgacc gaccgccact ggcgcggtcg gatcgctaat gtcgatgatc tcgagtccgc
    18181 cgatacccgc gaggtacgcg tgcgtaccgt cgatcatcaa gttcaagcct tcgatcacct
    18241 cgccgggaag acctttctcg agcgagccga cgagcgtgag cccggtcgcc agcgagtctt
    18301 ccttgtgcgc ggcgaagcgc tcgcccgtcg tgcgcgagat ccgttcgccg cggtcggtgt
    18361 cgtacgtgat gtatgtcgcg gcgagcgtgt cgctcgacgt caggcacgcg gcgtacacgc
    18421 gaatgaacac ggtgttgccg tactcgtacg cttcccgctg ccagtagcgc gtgccgtcgc
    18481 tccaggtgaa gaacgagtag ccatcgctcg cgtcgtgatc gtcggtgttg ccgaggcgac
    18541 tgaaccctgt cgtgcagctg ccttcgtact tcggatactc gaagcggaag tactgcgctt
    18601 gatcggagac gaaccagcgg ccaggtagat cgcgaaggcc atcggcgacg caattgctcg
    18661 gcagctcggc cgcgttcgga tcgatgtaca tgccggttgt cggcagcggt gccaccggtg
    18721 gcgtacacgg ccccggcatc atgtcgtcgc cgccacctcc attgtcgcca cacgcggcaa
    18781 gaatcaggac aaagctaaga accacccgca tggcggccag cctaacctga cgacgctcga
    18841 ctgcaacgct catacgcgct ggcaatgtgc gatgcgacag cagctgacac gcctggtcat
    18901 cgcccggtgc gtcggcgtta gtagaggtac gccatgacga gcccaatacc gagcacgaac
    18961 aacatgatgg cgacgacgag catgacgacg gagctgcgcg acgcctgatc gcgagggaat
    19021 ttctcggttg gttcggcgcg catgaccggg cggccggcgg gcagcgcgct cgcggcgcgc
    19081 ggcggtggct cggcaaatgc cggcatcgcg ggctcgggcc ggtagctcgc gagcgcggca
    19141 tcgagcgcag aggcgagcgc ctgcacgttg gcgaagcgtt cctcggaatc gtgggcgagg
    19201 cagcgcagca ccacctcgtc gacgacgtgc ggcaccgaga cgagtgtcga cggcggcggt
    19261 ggcgcttcgg tcagttgctg cgtgagcaga tcgagaccga cggcgttgaa cggacgccgc
    19321 ccgacgatca gctcgtacgc gagcacgccg accgtgtaga tgtcggtgcg gttatcggcg
    19381 cggccgccca tcaactgctc gggccccatg tactcgatcg tgccgactgc ggtgcccatg
    19441 atcgtgagct cttgcggagg cggcatgtcg ctcgtcgcga ggatcttcgc gatgccgaag
    19501 tcgagcacct tcacgacatc gtcgttcgtc aggaagatgt tgcccggctt gaggtcgcgg
    19561 tggatgacgc cgagtgcgtg ggcctcgccg agcgcgctgc acatctgacg cgcgatgccg
    19621 aacacgcggt tccacggcat gcggccgtgt agctggagca ggcgcagcag cgtttcacct
    19681 tcgagcagct ccatcgcgat gaacggcaac ccttcaggcg tgctgccgat ctcgtaggtc
    19741 gcgacggtgt ggggatcgcg tagcttgatc agcacctcgc cttcacgacg gaagcgagcg
    19801 acgagcttgt cgtccgcggc gagctcgcgg tgcatgatct tgagcgcgac cggttggccg
    19861 acgtccgtct gctcggcgcg gtagatcgat ccgaaaccgc cgatcgcgat ccgcttcttg
    19921 acgaggtagt gacctgcgat cacgaggccg atgtgtggat cgtcgacggt gcggccgcag
    19981 gtcgggcaga acgtcgcgcg cgagtcgagc gcggtggagc acgccgggca gatcatgcgg
    20041 gcaccaccga gggcagcggc gccgcaagcg catcgaggta gcgcgtgcgc agatcgatac
    20101 gaccgaggtg cggccggccg tgagcgcgct cggcacgagc ctcgaacccg agctgcgcac
    20161 cgatcgcgaa ccgctcgttc aaatagcgcg cgagaaccgg ccgggacacg atcttcgtcg
    20221 cgacgatgtg gcgcacgccg gtgacatccg aatgcacgag ctgccacacg cggcgcgcgg
    20281 catcctccgc ccacaccgcg gagcggtgct cgtcggcgac gacggtcatc ggcaacccgc
    20341 gctgggtgcg gccgcgcagc caatcgaggt ggccggtgcg accggtcgcc gatggcccga
    20401 tccacagacc ggcgcgaatg acgagcgtgt tgtcgcgcgc gagcacgagc tgctcggcgg
    20461 cgacacgcgt ctgtcccatc acgctcaccg gatcggtcgg cgagtcctcg tagtacggcc
    20521 cggtgtcgcc gctgaacacg tggtcgctcg agaggtgcac gatgcgggcg ctcgcgggtg
    20581 catgcgccag cagcacccgt gtgccttcca cattcaccgt gtgcgcgaac tccggcgcgt
    20641 cctcgcacgt gccgacgtcg cacacgccgg cgcagttgat gatcacgcgc ggtgtcatca
    20701 tgcgtgaccg cgcgaacagc gccgccaccg caggctcgtc atcgagatcg atgccctccg
    20761 cgatgccgtc cggcacttcg cgggtgagcg cgttgcaaaa agccgtgacg ctcgaagcct
    20821 tgccggcgcg caagatcgac caccccagca tggaggtcgc gccgaacaag aacacctcgg
    20881 acatgtcgcc cagtctagcc cagaggcagg cctagctccc tcagctcggc ctcacgaagt
    20941 ggtagctcgt cgcggaggaa gaattcgtcg agctgcggcg gcttcacgcg cgtgcccgcc
    21001 agcatcgcgt gcgcctggcc gcggtggtga atctggtgct cgagcaggtg cagaagcacg
    21061 ttcgcgcggg tgtcggtctg cacgcgcgtc tcgcgctgca tgcggatctc gtcctcggga
    21121 ttgccggcgg cgcacgcgac cagccgcatg tcgacgtcgc gctgcatccg gcgcagctcg
    21181 gcgaacgtgc cgctcttctc gaacgcctcc aacgcagccc atggccgagg gcggccgcct
    21241 gcctcgagcg cgtcgacata gaacaggtcg acgaacagga tgtgcgtcag cgtcaggtgc
    21301 agcgatggaa agaagctcgt gcgcgtcgcg cgatactcgg cgtcgtcgag ctcgccgacg
    21361 gcgcggtgca gtcgcagatt cgcccacgcg ttgttgtgcg ccatgcgcac gaggacgttt
    21421 gtcgcgttca tatcacctcc acgatcgcga gcgtgcgttg cgcgccaccc gcagtcagct
    21481 cgcattcatc atcgacaact ttgccgtgca gtgcgcgccc catcggcgag gtcggcgtca
    21541 cgacggtcac gtcgccgtcg accacgagcc cgccaccgtg cgcggcgatg aacagcgtgc
    21601 gcctgcgatc gtcctcctcg acgacgacga ccgcgccgac cgcgaccttc gtcaatggtt
    21661 ggatcgccat cgcgccgatc accgacaccg cggtctcgag atcggcgaca cgctgtgcgt
    21721 gaccgcgcgc gagatacgac tgctcgaggc cgcgcgtgtc tttgtcgttc tccggccgcg
    21781 cgtcgtcgct gatcgcgcct tcggtcgctg ccgcgtgtga tgcgcgcgcc gtcgcaagct
    21841 gcgcttcgag ctgcgcaagg agctcgcgcc ggagctcagc tttcgagcga ggacctgcca
    21901 tgcgcggcag tatagtgttg gtatgcggta cgcgctcgtc atcagcctcg cggcgtgcgc
    21961 cggatgcggt gacaacctgc cggagtgcgg cttcgttcac acgctggcgg cgaatcgcaa
    22021 catctggggc ggccacatcg ccgtcgacgc tgatcgcgtc tactacagcg actacgacaa
    22081 cggtatcggc acgcacctcg tgtttcgcca gccgcgcgag ggcggccaag cactcgtgat
    22141 tagcggtcgc gacgagagca gccgattcgg ctacggcatg gtgaccgacg acagctacct
    22201 gtactggacc gcggaggtcg cagaagccgg ctacacgctg ttcgcgacgc cgaaacttgg
    22261 cggcgcctca ctccagctca aggtcgtttc ggtgtgcacg gcgcacggcg tcgccgtcga
    22321 cagcgtcaac tcgtacgcag gcgcgatccg ctgcgacgac aatccagcgc aggtcgttgc
    22381 cgcgccccat gtcggtagcg gtggcggcgt gatctggagc tcgaccgatg ccgacgtttc
    22441 cgcgctcgcc gcgcgcgatg gcaacgtcgt gatcgcgacg acgaacggcc tgttccgtgt
    22501 gatgcccacg gcgaccgagc tcctcgacgg gcattcgacg tatcaggtcg tgatcgtcgg
    22561 cgacgagatc gtgtacagca cgaccgaggc ggtgatcgcg atgccgtacg caggtggtgc
    22621 gcagcgcacg ctgtatacgt tccacacgac aatcgatcag ccgcgtccgt tcgcggttga
    22681 tggtgacgat ctgtatgtcg tcgagtcgac cgagctcgtg tacatccatg ctggcgacca
    22741 accgatctcg ctcgtctcgg acatggggct ggcgatcacg cacatcgccg cgggtggtgg
    22801 tgccgcgtac tggccgacgc tcgcggtgcc gggtgcgccc ggcgtgctcg atacattcaa
    22861 cggcgccgtg ttccgcgtaa cgccgccgtg tcactgagga acgcgatcga gcgcggcgcg
    22921 gacgatctgc gcgagcatgt cgggctgaaa cggcttttgc aggaagttca caccgtcgac
    22981 cagcggctcg cccctcccag cctgcgcgga cgtgtagccg ctcatgtaca cgatgccgag
    23041 cgcaggggcc gtcgcacgca accgttcggc gagctcgcgc cccgtcacac cacccggcat
    23101 cacgagatcg gtgagcacca gctgcacctt gtcgcgaagc tcgggccatt gctcgagcgc
    23161 cttcgcaccg ttctgggccg cgtagaccgt gtagccgcac ttctcgagca ggttgacgac
    23221 gagcaggcgc aacgcgtctt catcctcgac gacgagtaac gtctcggtgc cgtgcggtag
    23281 gtccgcgtcg tcgccgccgc gcgcatcgtg ttgcgggcgg cgtgccgtcg tcgccggtag
    23341 gtagaagcag aacgtggtgc cgcgccccgg atcgctcgcc acatcgatcc agccttgatg
    23401 ctggcgaatg atgccgtaca cggtcgcgag accgaggccc gtgcccttgc cgacatcctt
    23461 cgtcgtgaag aacggttcga agatgtgtgg caacgcatcg ggtgcgatgc cgctgccggt
    23521 gtcactcacc gtgatgaaca cgtgtgggcc gcgcgggccg tcttcgccgc gcaccgcgtc
    23581 gggttcggtg atcatcttct cgccggtcgc gatcgtgagc tggccgcctt gcggcattgc
    23641 atcacgcgcg ttgatcgcga ggttgagcaa cacctgctcg agcatgccga cgtcggcctt
    23701 gaccggcggc agctgcgtcg agcaatgcgc gacgagcgtg atcgcttcgc cgagcgcgcg
    23761 ctgcagcatg cgcgtcatgt tgcggacgac gtcattgacg tcgacgatgc gcggctgcaa
    23821 cacctgcttg cggctcacca tcaagagctg tcgcgtcaat gtcgcggcgc gctcgctcgc
    23881 ctggatgata tccgcggcat gctcgcggat agcgtctccg gtgctcggcg cacgcgtgat
    23941 cagcgatgca ttgacctgga tcacggtgag caggttgttg aagtcgtgcg cgatgccgcc
    24001 ggagagctgg ccgagcgcct ccatcttctg ggcctggcgc agctgctcct ccaggcggcg
    24061 acgttcggtg acgtcgagca tcgcgccgac catgcgtacc gcctggccgc gttcgtggat
    24121 cacgtaaccg cgatcgagca cgttcgcgta gtggccgtcg tggcagcgaa agcgatactc
    24181 gtcggtccag tagtgctggc cactctggat cgcaccgaag aggctgcgct cgacgtgcgg
    24241 aaggtcgtcg ggatgcatgc gtgacgacca cgcatcgatc gtcgatacat tttcgggcgc
    24301 tacgccgaac agcgtgcgca ttccttcgtt ccaccagatc gcgtttgttc gcaggtccca
    24361 gtcccagacc gcgtcgtttg tcgcgcgcgt gacgagctgc aagcgttcgt ggccgacccg
    24421 gcgttcatcg acgatgcgct tgcgcagctc gagctgcgcc accacttgcc gcgcgagcgc
    24481 cgcgagccct tcgatctgaa ccgatgtgag cgatacgcgt gggctcgtgt cgaccacccc
    24541 gagcgtgccg aggcattggc cgttcgccat cacgagcggc gcactcgcgt agaagcggat
    24601 gcccgcttcg gcgacaaacg gcgtctcggc aaatcgctcg tcctgtgtgg tgtccggcac
    24661 gatcatcacg tcggggccga ggatcgcgtg cgcgcagaat gcgaggtcgc gcggcgcttc
    24721 agtcgcgggg aactgatacc gcgccttgaa ccacacacgg tctttgtcga cgaatgtgat
    24781 gaccgcactc ggcgtgctgc agatgacggc ggcgagcttt gcgagcgcgt cgagctcggg
    24841 ctcggagtcg ttatcgagca cgtggtagtc gcgcagcgtc gcgagccgaa ctggctcgtc
    24901 cctcgggcgc ggcgccgctg tcatcccact gcgatcaata gccgatcgcg gacgcttggg
    24961 cagtgatcaa acggaaagac cggatcgaga caacgctacg cgtcgcgaag tttcagcccg
    25021 gctgcaacct ttttcgcctt gccgagtgcg gcttccgcgg tatccgcagt agccagtaca
    25081 actccgagtc ggcggcgcgg caccgcgttc ggcttaccga agaagcgtac gtcgatatcg
    25141 ggcgtcgcgt aggcctcgcc gacaccgtcg agcactggcg acgcgagctt gccctcggcg
    25201 cgcaacgcga cactggcacc ggcggcgtag cgccggatgt tcgcgatcgg cagtccgaga
    25261 atcgcgcgcg catgcagcgc gaactcgctc caggtctggg tcgcgagcgt gaccatgccc
    25321 gtgtcgtgcg ggcggggact gacctcgctg aaaatcacgc gatcatcggc gcacaagaac
    25381 agctcgacgc cgaagatccc gtagccaccg agccgatccg tgatcttgcg agcgatcgcc
    25441 tgcgagcgtt cgatctgcgc agcattcatc gtgtgcggct gccagctctc gacgtaatca
    25501 ccgccgacct ggcgatgacc gaccggatca cagaagtgcg tgccattgac cgcgcgcacc
    25561 gtgagcatcg tgatctcgct ctggaactcg atgaactcct cgacgatcac gcggccggcg
    25621 ccggtacgtc cgccggtctg cgcgtagtgc cagctctgct cgagatcagc ctccgtcttc
    25681 gcgaccgact ggcccttgcc cgacgagctc atgatcggct tgatcacgac cggccagccg
    25741 agctcgcgcg ccgcggcctt cagctcgtcg aacgtatcgg cgaactgata acgcgcggtc
    25801 ggcagcttca gctcctcggc cgcgacccgg cgaatcgcct cgcggtccat cgtgagccgc
    25861 gcggcctgcg ccgtcgggat cacgtgccag ccttcgttct cgagtgcgat gagctcctgc
    25921 gtcgcgatcg cctcgatctc ggggacgatc agatgcggct tctcgcgctc gatcacggca
    25981 cgtagcgccg cgcgatcgag catgtcgatc acgtggctgc gatgcgcgac ctgcatcgcg
    26041 ggggcatccg cgtagcgatc acaggcgatg acttcgacgc caaatcgctg gagctcgatg
    26101 acgacttctt taccgagctc gccggaaccg agcagcaata cacgcgtcgc gttctcactc
    26161 ttgggcgttc cgatcgagac catgcgcggg ggcataccac gcgcgtctgt cagcgcatca
    26221 gaacaatccg gcgggtaccg accagtacgg actgatgttg tcggccttca tgtcctggcc
    26281 gggcaaccac atcggcgccg cgctcgggtc gacggtgccc gacagcttgt tcttggcgac
    26341 cgcaccgatc cagatctgct tgcactgggt gacgccgttc gcgacgcacg atggatcggt
    26401 gttcgcgctg gtcagtcgat ggccgtagtc gcgctcggtc gagaacacga tccagtagta
    26461 gtcggtaccg acggtcggcg cccagtgcgc ccacgtcgtg tcgagatcgg cgaagccgcc
    26521 gttcatctcg gtcatgtcga cgatccacgc gccacccgca tccgcgagca tcagacgctg
    26581 cccggcgtcg cgcgccggat tcgagcgcca ggaggtgcgc gacgcgttga gcacgatgag
    26641 agcgttgtcg ggcgagaagc tcggaaagaa gaagttcgtg tagctgccgt tctggagcgt
    26701 gatcgggttc gggaacggga acgtcggcac gccgaacgtg tgctggccgc ccgtgtatgc
    26761 gtacgacatc atcgcgatcg acgagccgga gagatcgatc cattggttcg ggttcggggt
    26821 cgaggcgaac acgatcttgt tgccgtccgg tgaccagtca gccatcagcg ccgagcgacc
    26881 gctcgtcgga cccatcgtcg agacctggtt caagttcgac ggcacgacca tgccggtgtc
    26941 cgggtcgtag agctggagcg tgccgttgac catcgagacg atggccgcag tcgattgctc
    27001 ggtgaatggg ccggtgccgt tcggcgggaa cgtcgtgtac gagccgtgga tgccgaggtt
    27061 gttcgcgttg acggtctcca tccagctctg cgatgcgccg tcgtacttga ggaagccggt
    27121 gtagaggttg ccgcagtcgt tggcgacaca ccgcgagtag ccgatgcgcg tgccgctgcg
    27181 attcacggtg tggcaggacg tgcagccgtc cttgacgagg ttttgcgtcg tctggccgaa
    27241 cgtctgttcc atgatgttgc cgcgcgacga cgcccagtaa tagatggcgg tcttgtcgat
    27301 gatgtcgcgc gcgatgacgt tcgtccgcgg cgtgctcgtg aacttcatgt cgggcgtcgc
    27361 ctgcgcgaga ccctcgacgg tgtacgtcag cgtctcgccg gcgctgctcg ccatcacgtt
    27421 ttcccagtcc tgctcggaga gctgcgcctc ggggctggtc gtgtagatgt tgaccgcgac
    27481 aaacgacgac gtcagcgaga tgtggaacag gtcgttaccc gcggccgtcc actgcgcctc
    27541 gatcggcggc atgttgcgcg gcgacaccgc gccctcgagc ggatactcga tcaccggtac
    27601 gcgcgcggcg tcggtgccga cggtcgcgtt gccgaacagg tcgggagcat cggttggggc
    27661 gggcggtacg acgacatcgc cgacgagatt gacgccgagg agtgcagtgc ccgtgagcgt
    27721 gttgcagatc gcggtgatcg tcgtcttgcc gccgtgcggg agcacggtcg cggtcgcgtc
    27781 gacgaacgtg ccgaagtctg cgtccatgct gagcgcgcac ttcagtgtga tgtcggtctt
    27841 ctccgaaccg ttgacgccgt agaccttgta gtcctgcgtc gcggtgccgc cgagcgggac
    27901 ggtcacggtc gtgaacgccg gctcgacttc gatcgacatg tagtcgctgt catcgccact
    27961 gtgcgagcca caggcggcga gcacgcagat cgctaggcaa agtcccctca tggcctatgc
    28021 ttatcgcggg ccgtgacgac tcgtcgagac attctgcgct tcgcagcggt aaccagtctg
    28081 ttgcgaggcg cccgcgtatt cgcggccgag gccaagatga ccctgaacac ccgtgcgatt
    28141 cccaagagcg gcgaacagtt gcccgtcgtc gggctgggca cctggcagac gttcgatgtc
    28201 ggggccggcg ccgacgagcg caaggccgtc cgcgaggtcc tcgccaagtt cctcggcgcg
    28261 ggaatgaagg tgatcgacag ctcgccgatg taccggcggg ccgaggaggt caccggcgac
    28321 gagctggccg gcatcaagga tgcgtcgccg tttctggcca cgaaagtttg gacgcgtggc
    28381 aaacaggccg gcatcgaaga gatggagcgg tcgttgcgca gaatgcgcac gaagacaatg
    28441 gacttgatgc aggtgcacaa cctgctcgat gtgaagacgc acttgcccgt gctccgtgag
    28501 tggaaggcgg cgggcaagat tcgctacctc ggcgtcaccc actatcagca cggcgaattc
    28561 gacgcgatcg agaagctgat gaagaccgag aagctcgatt tcatccagat cccgttttcg
    28621 atcgtcgacc gcctcgccga gaagcgcatc ctgccgaccg ccgccgacac cggcacggcc
    28681 gtgctcgtga tgacgccgtt cgaatccggc gagctgttca cgaaggtgaa aggcaagccg
    28741 ctgcccgcgc tcgcggccga tctcgattgc acgagctggg cgcagttctt cttgaagttc
    28801 atcatcggcc atccggcggt cacgtgcccg atcccggcga ccgcgaaggc gtcgcatgtc
    28861 atcgataacg tgcaggcagc acgggggcgg cttccggatg cgaagcagcg cgacaagatg
    28921 gcggcgacat tcgtgcgaac atagccagcg atggcagcag tgatcgtcga gctcgcggcg
    28981 cggaagttca cgcttcccgc cgagtgcccg tgttgcggtg ctcctccgga tagcgagctg
    29041 tccgtgacgg cgcaggcgac gcgccaatcc ctcgcgttcc cgtattgcag gcgctgcact
    29101 ggacacctgc agcagtggga gacctccggc gtcgcgtcgg cgggcctcat ggtgctcgcg
    29161 atcctcgcgg tgatcggcct cgcagttgcc ggcatgttct tgctagcgat cggcgtcttc
    29221 gtcgtcggtg catcgctcgc gtgggtgttg cggtcgtcgc ggcgcgcggc tgccaaggcc
    29281 gcgtgcggtg agtcgtgcgc gacgccgggg caagcagtca cctatctcgg ctgggccaac
    29341 gcgacgcacc ggttctcgtt cgcgtcgccg acgttcgcgg cgcgcttcgc ggaggccaac
    29401 caatcgctgc tcgcgaacga gacgccgcag ctgcgcaagc tcctcgacgg ttaccggaag
    29461 gcgcgtctcg ccgttcccac gcctgccgtc gctgctggtg ttgcgccgcc gccgctgact
    29521 gcgcgcgatt ggaccgcgcg actcgagtcg acctccggca ccgtgatgcg gcgcatcgcg
    29581 ctcctgcggg cactcgagat gatcgaagaa ccccaaccgc gccgcgagct gatccagacc
    29641 gtggcccgga tcgagctcgc gcccctgctc gacaagctac aacgccagtc gagccccgcc
    29701 gccaaacgct cactcctcaa cgccgccatt gccaagattc gcgaggacaa catccccgat
    29761 gagctgcaag cggccgagct cgagaagctc gaggcccggc tcgccgagct gtaactacca
    29821 ggtcatgccc acggtcatgc ccatgcggtg attgccttcg gtgtcgggca cgaagaagaa
    29881 gtggacgggt gtctggatgc tgccgaccga gggcgtccag cctgccgccg cgatcatgtg
    29941 cgacggcgcg tccggatccg gcgtgaggtt gacgccgacg ccgagctgga agctgcgatc
    30001 gaactcgaag ccgatcaggc cgctcgcggc cgggatgaac ttgctctgct cgagaccggc
    30061 gacggtgacg ttgccgacca tcagcacgtt cagccacgag tgaccgacga tgcgatagaa
    30121 gccctcgtag ccgagcagca tcatgttcgg cgacttgagg ccgtacttct cgccgatcgt
    30181 gaccggcatg ccgttgtcat cggtgcgcgt cttgtactgc gcatcgttca tcttgaagag
    30241 gtaggtccag ccgagccgga agccgtgcat gaagcggcga ccgaccgggc gcttccagcc
    30301 ttcgtcgtcg ctcggacccg acgcctcggt cgcactggtc gacgtatccg gcgcgagcac
    30361 cgcgacctgc ggctgctgct gcaccggctg cggctcgtca acgggaagga gcggcggcgg
    30421 ctgcggcgcc gatgcaggct gcgccgtggg cgtgttgcag cagttgcccg gtgcttgcgg
    30481 cgccggcgcg ggcgcggccg gctgggcgat ggcaacgttg gcacccgtga gggcgaagag
    30541 ggtggcgacg gtggcgagga cggtcttcat ggcgtttcct ttcgtgtcgt gctttcgaca
    30601 ctcacacccc cgcgcgtgaa catgcccggc gccccgcgtg aaccacgcgt gaagcagggg
    30661 cgaccaacgc tgtcgccttg gctcgacgac tgcatcgcta actctcgcaa tgtccgcgac
    30721 ttttcttggg cgcacgtgct cccacgcccg cgcgggcgca gtcgtcgcaa tcctgttggc
    30781 aaccgcgtgc cgatcccagc cagccgcatc gcggttgttc gtttcgaacg agagcgcggg
    30841 cactgtttcg gtgatcgacg cgcactcgac taagacgctc gcgacgattc ccgtcggcga
    30901 tcagccgcgc ggtctacgcg tatcgcgcga tgggcgcacg ctgttcgtcg cattgcgcgg
    30961 ggcgcacagc attggcgtcg tcgatctcac ccagctgcgg ctcgtcgata cgatcccgag
    31021 tggacgcggt cccgaggcat tcgatctggt cggtgatcga ttcatcgtcg tgtcgaacga
    31081 ggagacgcag gcggcaacga tcgtcgacgt gaatgcgcgc aaggtccgcg cgacgatcac
    31141 ggtcggcaac gcacccgacg gcgttgctgc agcacccgat ggttcggtgt ggatcacgaa
    31201 cgaagccgac aacacggtgt cggtgatcga tccgcgtgca gcacgcgtcg ttgcgacgct
    31261 cgcaaccggc atgcgaccgc gcggcatcgc gttcaatcac aagctcggcg ttgtcaccgg
    31321 tgagagcgac gcgtccgtca ccatcgtcga cattgcggca cgcgaagcga tcgctcgcgt
    31381 gcagttgccg atgacgggcg tgactccgcc gcagcccgtg ggtgtcgcga tcgatgctgc
    31441 gggccggtac gcgtacgtga cgaccgggaa cgccggcagc atcgcggtga tcgatctcga
    31501 agcgcatcgc cttgcccgag tgatcgagca tgtcggtcat cggccatcgg caatcacgct
    31561 cggtcgcgac ggcttgctct acaccgcgaa tggcccgggt aacgacatcg ctgcgatcga
    31621 tccgaggagc ggcctcgtcg tgcgccgcat cgccgccggt gattcgccgg gagacatcac
    31681 gtcgctgtga ggaggaccca tgatccggaa gttgtcgtcg ggaaagtatc ggctgtactc
    31741 gcgcaagaag gatgcgaaga cgggtaagcg caagaacctg ggcacgttca agtcgaaagc
    31801 ggacgccgag aagcacgagc gtgccgtgca gttcttcaag cgccgcggct agcgatcact
    31861 tgttgatcgt gacgtcatcg cggcggtttg ctcgacggac ggggcacgag ctggcgaagc
    31921 tgccacgatt gcagctgcat cgtgcgccgc accttgcgtc tgtcatcgtc ttgcagccgg
    31981 gagagcaggg cggccaggtg aatgagcgcc acgatgtaga tgaacaggcc gaccagcgtc
    32041 gcattcggat cgagctcgcc cgcgggtgtc tgcaagagca gcgtgttgcc gctcaccgac
    32101 acgcgatcgg gcaacacacc cgcgatggcg agcagccacg gcgatacggt tgcaatcacc
    32161 gtcagtagtg cgaggagcca cgggcggatc agctttcgat gtgtcgccat cagcgacacc
    32221 atgatgatcg ccggaccagg gccgatcacg atcgggctca ccatccagcc gaacaacgca
    32281 aacatcgcga ggttcagcgt gatcgtgatg tagcccgaga cgtagggatt gcgcggcgcg
    32341 atcaacgctt gcacgaggat gatcgtgaag cagatgagcg gaccaacgat gttgtaccag
    32401 gcgtcacgaa agccgatgta gtagaggacc gggaagaacc cgaggtatgc gatcgcggcg
    32461 agcatgccga agcgggcgct gatcttgagc gcgtcctcgt cgatcgctgc gagctcctct
    32521 tcgacctcgg gcggcatcac ggtcggtgcc tcgagcatca gtgcgccgac gaggtcggcg
    32581 ggctcgcggt ccgtcgggtc gagcgcgagt gcgcgtgctg cagcgcggat cgcatcgcgc
    32641 cgctcctcgg ggccatcgcc cttgccgagc gctttgcgcg cggtcgccat ctcggccttt
    32701 gcgagctcgc ggcgtagcgc gacgtcgcgg ttgccgtcga ggaaacgctg cacggcggta
    32761 ccgagctcgc gcgcggtgtc gtagcgcagc tgcggatcga cttgcgtcgc tttcacgcag
    32821 atctgatcga gctcgggcgg aatctcgcga ttgggtgcgc ggaccgaagc gcgcgcgtcg
    32881 acgcctgcga gtgccgacgc gatgcccggt tggccacgcg gatgcagcgg cgcgagcgcg
    32941 aggatctcga acaggatgca gccgagcgag tacacgtcgg cgcgaccgtc gaggaagtgg
    33001 tcgccgcgga tctgctccgg cgagatgtat ccgggcgtgc cgaggatcgc gccgaccacg
    33061 gtttcggttc cggccgtcgt tgtgatgtcg gccatcggcc ggatgtcggc catcgtcccg
    33121 atgtcgtcga agctgtcgcg cttcggctcg ttcgggtcct cggtgatgcg ggcgatgccc
    33181 cagtcgagca cgtagacttc accgtaatcg ccgagcacga tgttcgccgg cttgagatcg
    33241 cgatgcacga ctcggcgcgc gtgcgcgaac tcgatcgcga gacagacatc ggcgaacgcg
    33301 cggagcaggc gctgcagtgt gaactcgtcc gccgcgcgct ggtcgcggcc cgcgagcttt
    33361 ggaatgacgt cggcgagtgt cgtgccggtc agctgcttca tcacgaagaa cggctgaccg
    33421 ccttcgttcc acagctcgtg taccggaacg accgcaggat gttcgagccg gccctggatg
    33481 cgtgcttcgc gcaggaagcg agccatgatc tcgggcgtcg ggttctcgac ccgtagtcgc
    33541 ttgatcgcga cctggcggcc gatctgttcg tcgcgggccg acagcacctc gcccatgccg
    33601 ccttggccga gcacgtcgcc aatccgatag cgctcgagca tcgagccgat gtctggcgtc
    33661 gccaccggtg gagcgccgat cgtggtcttg tcgccatcga cgagcgtgct cggatgcgac
    33721 gcttcgaggt cgatcgcgcc atacaggcgc gtcacgcgcg gtcgactgga aggatcgctg
    33781 tcgccatcgc gcgaatcgcg gccgtcaccc tccatgtctg catcgtatcc cgtgccgcta
    33841 cgtcgctgca cctacacggt gtgatcgccg gaatcgcttg cggctaggta aggttccggg
    33901 gcgacgatcc cgcgaccgcc cgtgtcgatc gcaccgtcgc cgcggtcgcc gcaatcgccg
    33961 gctgacgata tgctgcccgg aatgcggctg ctgttgctcg tgctcgcact cgtcggcgtc
    34021 gacctgcggg tcgtgtacgc gggcggtgcg tgagcgggtt gaagcaaccc aaacctcccg
    34081 gtcgggaagg tcgacagtga cgcgatgccg ccggggcagt tcatgctccg gctcggctgg
    34141 ctcggcacgt acgtgcaaac ccagcacccc gacttcgccg acgtcgtcgg gcagggcacg
    34201 attccgatca tccatgatca ggacatcgcg atcaccgaag cgcggctgtc gctcgacgct
    34261 ggcctgacca agcggttctc ggcttcgctc gtcgtgccgt tccgcgtcgt gtcgacatcg
    34321 atcgtctatc gcaacggcat gggcatgccc gtcgagctcg ccgatccgaa catccaccac
    34381 cgcaacgaga cgctgaccgg catcgcggat ccgatcgtgc tcggcgcgtt cacgtacgcg
    34441 ctcggcgacg tcaggttcac ggcgcgcggc gggctctcga tcccg
//
LOCUS       DASUFX010000630         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_795839,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000630 DASUFX010000000
VERSION     DASUFX010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795839"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFV99_25415"
     CDS             complement(<1..313)
                     /locus_tag="VFV99_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062747.1"
                     /translation="MLRLAALIPLALVACVDTGDEGIFVLTNTAVSSGTCSLSGDPSQ
                     PQIGHGTINSNSPIAYVMTPLIQSRIQPIEGVDNSSKTVQLRGADVKLTLKAVTIQNP
                     PG"
     gene            complement(338..1924)
                     /gene="aroB"
                     /locus_tag="VFV99_25420"
     CDS             complement(338..1924)
                     /gene="aroB"
                     /locus_tag="VFV99_25420"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5062748.1"
                     /translation="MPRPLFLVGFMAAGKTTVGRLLAQATHRRFIDLDDVIAEQGESV
                     AALVARDEAEFRRREAAALAHVIADSTDTPIIATGGGCAAYGDNMTRMRAAGLVVALD
                     VHIDTAQARAAGGPPRPLLASAAELAAKRAPAYRRAHAIVDTTHRSLLEVVAQVAEVE
                     RAWQRLPFAHRDATLLALDERTYPVVVHERAIDGELIRTFLEGASRVVIITDHEVGRY
                     WQTSAIAALSGAPDVLSIEPGERSKSLAVYSQICEALVAGGLDRGSVLVALGGGVVGD
                     LTGFIAATMFRGIPVIQLPTTLVAMTDAAIGGKTAVDLDAGKNLVGAFWQPRLVACGL
                     ETLSTLPARERRAGFGELWKYALLDGETLWQLVDTCAPWAAGETTDPPPALRDVIQRS
                     IAYKAAIVGRDEREVTGQRALLNLGHTVGHAIESATGLHHGEAVGLGLIAAARVSAAV
                     VGARKSLEGEVTAALRRTGLPAELDPLLTDDVLNRIKVDKKRIGDKVRFIAIREVGAC
                     DPVEITVTELRRILRPDSAP"
     gene            complement(1984..>3022)
                     /gene="pilQ"
                     /locus_tag="VFV99_25425"
     CDS             complement(1984..>3022)
                     /gene="pilQ"
                     /locus_tag="VFV99_25425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX5062749.1"
                     /translation="EAARRAAAAASEMPKPETLILNYASADELRPKLEGMLSAKGKIE
                     TDGRMNALIISDIAANRAEIMRLALSLDIQTPQITIEARIVEARSTFVRQFGIQWGGR
                     ALASAATGNATGLVFPSSVGVRGGNDDNQTRDGGITALPTDFAINLPAATGTGEGGAI
                     GLSLGSVGGNFNINLRLSALEDTGSVRIISAPKITVLNNKAATIKQGVSIPISVVSAA
                     GTMTQFVQADLALEVTPYVSQRDCAIAMNLSVTKNEPDFVNTGARGDPTILRKEAKTT
                     MLVADGETSVLGGIYTRNTGLAYKKVPFFADLPVIGWFFKSRRENDDRTEILVFITPK
                     ITNKAQLPCAQ"
BASE COUNT          477 a          971 c         1051 g          523 t
ORIGIN      
        1 aaccgggcgg gttctggatg gtcaccgctt tgagtgtgag cttcacgtcg gcgccgcgca
       61 gttgcacggt cttcgacgag ttgtcgacgc cttcgatcgg ctggatacgg ctctgaatga
      121 gcggcgtcat cacgtacgcg atcggcgagt tgctgttgat ggtgccgtgc ccgatctgcg
      181 gttgcgaggg atcaccgctg agcgagcagg tgccggagct gactgcggtg ttggtgagca
      241 cgaagatgcc ctcatcgccg gtgtcgacgc acgcgacgag tgcaagggga atgagggctg
      301 cgaggcgaag catggacgtc ctccaacgaa aatcgtatca aggtgccgaa tcggggcgca
      361 aaattcttct cagctcggtg accgtgatct cgacgggatc acacgccccc acctcgcgaa
      421 tcgcgatgaa ccgcaccttg tcaccgatcc gcttcttgtc gaccttgatg cggttcagga
      481 cgtcgtcggt tagcagcggg tcaagctcgg ccggcaaccc cgtgcgccgc aacgccgccg
      541 tgacctcgcc ctcgagagac ttgcgcgcgc ctacgacggc ggctgacacc cgagccgccg
      601 cgatgaggcc gagaccaacg gcctcgccat gatgcagccc ggtcgccgat tcgattgcgt
      661 ggccgacggt atggccgagg ttgagcagcg cgcgctgccc ggtgacctcg cgttcgtcgc
      721 gaccgacgat ggccgccttg tacgcgatcg agcgctggat gacgtcgcgc aacgcgggtg
      781 gcgggtcggt ggtctcgcca gccgcccacg gcgcgcacgt atcgacaagc tgccacaacg
      841 tctcgccatc gagcagcgcg tacttccata actcgccgaa gccggcgcgc cgttcgcggg
      901 cgggcaacgt gctcagcgtc tcgagcccgc acgcgacgag ccgtggctgc cagaacgcac
      961 cgacgaggtt cttgcctgcg tcgaggtcca cggcggtctt gccgccgatc gcagcatccg
     1021 tcattgcgac gagcgtcgtt ggcaactgga tcaccgggat gccgcggaac atcgttgccg
     1081 cgatgaagcc cgtgagatcg ccgacgacac cgccgccgag cgcgacgagc accgagccgc
     1141 gatcgaggcc gccggcgacg agtgcctcgc agatctggct gtagacggcg agcgacttgc
     1201 tgcgttcgcc tggctcgatc gacagcacgt cgggtgcgcc gctgagcgcg gcgatcgcgc
     1261 tcgtctgcca gtagcggcca acttcgtggt ccgtgatgat gacgacgcga ctcgcaccct
     1321 cgaggaacgt gcggatgagc tcgccgtcga tcgcgcgctc gtggacgacg accggatacg
     1381 tgcgctcgtc gagcgcgagc agcgtcgcgt cacggtgcgc gaacggcagc cgctgccatg
     1441 cgcgctcgac ctcggcgacc tgcgcgacaa cctcgagcag cgagcggtgc gtggtgtcga
     1501 cgatcgcgtg ggcgcggcga tacgcaggcg cgcgcttggc tgcgagctcg gccgccgatg
     1561 cgagcagcgg acgtggtgga ccgcccgcgg cgcgggcctg cgcggtgtcg atgtgcacat
     1621 cgagcgcgac gacgaggcct gccgcgcgca tcctcgtcat gttgtcgccg tatgcggcgc
     1681 aaccgccgcc ggtcgcaatg atcggcgtgt cggtcgagtc ggcgatcacg tgtgcgagtg
     1741 cggcggcttc gcggcggcga aactcggctt cgtcgcgcgc gacgagcgcg gccaccgact
     1801 cgccctgttc cgcgatcacg tcatcgagat cgatgaaacg gcgatgggtc gcctgcgcga
     1861 gcaagcgacc caccgtggtc ttgccggcgg ccatgaaccc gacgaggaac aagggccttg
     1921 gcatggcgag attgtaatcc gagctgcgcg gtgaggctgg ctactgaggc cgcctctcgg
     1981 cgctcattgc gcgcacggca gctgggcctt gttcgtgatc ttcggcgtga tgaacacgag
     2041 aatctcggtg cggtcgtcgt tctcgcggcg gctcttgaag aaccagccga tgaccggcag
     2101 gtccgcgaag aacggcacct tcttgtatgc gagaccggtg ttgcgcgtgt agataccgcc
     2161 gagcacgctg gtttcgccgt cagcgacgag cattgtcgtc ttggcttcct tgcgcagaat
     2221 cgtcgggtca ccgcgagcgc cggtattgac gaagtccggc tcgttcttgg tgacgctgag
     2281 attcatcgcg atcgcacagt cgcgctgcga gacgtacggc gtgacctcga gcgcgagatc
     2341 ggcttgcacg aactgcgtca tcgttccggc ggccgacacg acggagatcg ggatcgacac
     2401 gccctgcttg atcgtcgcgg ccttgttgtt gagcaccgtg atcttcggcg ccgagatgat
     2461 gcgcaccgag ccggtgtcct cgagtgccga tagccggagg ttgatgttga agttgccgcc
     2521 gaccgagccg agcgacagac cgatcgcacc accttcgccg gtaccggtgg cggccggcag
     2581 gttgatcgcg aagtcggttg gcagcgcggt gatgccgccg tcgcgcgtct ggttgtcgtc
     2641 gttaccgccg cgcacgccta ccgacgatgg gaacacgagg ccggtggcgt tgccggtggc
     2701 ggcgctcgcg agtgcgcgac cgccccactg aataccgaac tggcggacga acgtggagcg
     2761 cgcctcgacg atgcgcgcct cgatcgtgat ctgcggcgtc tggatgtcga gtgaaagcgc
     2821 gaggcgcatg atctccgcgc ggttcgccgc gatgtcgctg atgatcagcg cgttcatgcg
     2881 accgtcggtc tcgatcttgc ccttcgccga cagcatgccc tcgagcttgg gcctgagctc
     2941 gtcggcggat gcatagttca agatcaacgt ctcgggcttc ggcatctccg acgcggccgc
     3001 agcggcgcgg cgagcagcct cg
//
LOCUS       DASUFX010000631         9318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1074157, whole genome shotgun sequence.
ACCESSION   DASUFX010000631 DASUFX010000000
VERSION     DASUFX010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074157"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFV99_25430"
     CDS             <1..500
                     /locus_tag="VFV99_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062750.1"
                     /translation="AHYRAVIAPTQNRIDRGLWQRLRGLAEHKKAIVVIGPTTPTRDE
                     LDQPLDEPAPKRIGKLNEGSLDDIVGLADDLVALAGELSDDWHVERPDDVRTMAYADA
                     SGATRVVFVVSDAARPVTAVVLASTAVTSVRDPLANEELPIDDGRVRVAMGPRGVRML
                     VVGGS"
     gene            500..1468
                     /locus_tag="VFV99_25435"
     CDS             500..1468
                     /locus_tag="VFV99_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5062751.1"
                     /translation="MKKLVSIAALVALVACGGRGLNLTMIDASVQRPSNVAVYFTVDT
                     ADGEPVPGLNVMAFKIYEDGAPISALESRQTILNPEVSASHYTLLLIDMSGSVTGSGD
                     VPVIVEAAKGFSQRVEKFQKVSIYAFDGSPTIRQIGSPDQLSSFSAKDPSTNLNGAIV
                     EGLKVLHQQMSSSSTPLTFGTLVVFTDGTDRAGRVTRDKLYETLDQAQVDILTIGVGA
                     EIDGGELRNIGRNGAIVNKDRAQIAASFEAAAARVEAMSNRYYLLGYCSPARAGKHTV
                     RIETNANGKSGSLEYEFDAKGFGPNCDPNRPPSFNIRRPKRVAVQP"
     gene            complement(1465..1878)
                     /locus_tag="VFV99_25440"
     CDS             complement(1465..1878)
                     /locus_tag="VFV99_25440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5062752.1"
                     /translation="MGSILLVESDPDTRDRLGSAMREAGHEVLVAITLREAYLRISEG
                     GIDAVVIDSYDPRVGVTDLAKSVNALPDGPPLVLVSPSPYAPEISARIGATAFLPKPC
                     EPSELVAIVDRIAGEVRPVRNFEDEDPTGPVRQTG"
     gene            complement(1965..5027)
                     /locus_tag="VFV99_25445"
     CDS             complement(1965..5027)
                     /locus_tag="VFV99_25445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062753.1"
                     /translation="MSVQDQVQKSLKLVGRVLGDKFKLTACIGIGGSGAVYKADQMAL
                     GRTVAVKILNEELCADPRMITRFRGEAMSASRLNHPNCVSIIDYGQAPDGLLYLAMEY
                     VKGPTLTQLLVNEHPLGVERVIDVVCQSLAGIEEAHLAGVVHADLKSDNIIVDQRRAG
                     IDLVKIVDFGIARLVTGVREAEDRSISGTPEYMAPEVISGAPPSFASDIYAVGIILYE
                     LLAEKTPFFASSTTEILTNHLKVAPTLLTTRREGVPREIDAVIGKALAKHPSDRYSSA
                     HEMREALLGIRARMRALPVTDKCESCGASVTPTFKFCPECGAPRIRISKTFEVPAATP
                     APSDALPLPFCGRVLEKQQLLDHMKRAPGSNGGLLLVGLEGAGRSTLLRKVYPELLAD
                     SRVVYQIGPDPSGLAAPFYPIRSLLAAILQLPALSSEMELREAVLAVGLNERDVPGIA
                     QLFGQPTTLLELEAPVRRREMVWSALRAIERAAVDAKVAIVCEDVDRFDEPSLEILRR
                     ATESGDLALPPVVMTATPAFSQAWPASVKRLEVGALDAKDLEAIISKVAAAGVRGLPP
                     VQQLFETTRAYPGHVDHVVRYLLEGGRAEDTRVKLPDLVAARLSMLAQSTRDVLQAAA
                     VLGIEPQLDLLRSMMPTETLEPALVDAENHGVLGKHPSGELLFTSKLVREIVYDATPA
                     DVRRSLHASAAAAVQSLSHDIGLLGHHHDLAGHAKDAVDLLRAAGDHAAEQLDEHGAG
                     QFFYRALVAVRQAVQSGDIEDGAEQQFVAMSVRLADVLRTRGETALARGILAEARDWS
                     SAPLLVSMIDRAGAAIALSEGDIDGAIAALRRGVGRAIGSGDMNLVCELYLDLAMALQ
                     RAGDPETANRELVECVDLATLGEGFTATEGTESFWRILRAQAQLIDAAGDSYRALRLA
                     EAALFHAQRVRSRLGAARVQQLLSALCEKAGLGGKAERYRQAAINEMRNLGDRRATAE
                     LLLTETPSRGAVVQPNRYQDAMQLTKEIGWAEGHERAKRKSQPPTSGS"
     gene            complement(5062..6333)
                     /locus_tag="VFV99_25450"
     CDS             complement(5062..6333)
                     /locus_tag="VFV99_25450"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5062754.1"
                     /translation="MASTVGTSAPVRQFSAYGARMTRAAFIVDGIRSPFGRYGGGLGG
                     VRADDLAAAVIAALVARTKIPTERIDDVILGCANQAGEDNRNVARMAALLAGLPVSVP
                     GITVNRLCGSGMQAIADAARLIMTGEADLVIAGGVEQMSRAPQVMAKPTDAFPRGNQT
                     IYDTTLGWRFINPRMEQMHGTLAMGETAEKVAERCNVSREAQDRFALLSQQRAGKAIA
                     SGRIAEEITPIVTGVDKKTGAETKVTMDEHPRPETTIEQLQKLRPAFAKNGSVTAGNA
                     SGLNDGAAALLLASEPAMKELGLAPKARYVAGAAAGVEVSYMGLGPIPASKKALTLAG
                     VTPQRIDLAELNEAFAAQAIPCIDQIGLDPERVNVNGGAIAWGHPLGASGARLVLTLM
                     TELSHRSGQYGLASMCIGVGQGIAVVIERTS"
     gene            6332..6724
                     /locus_tag="VFV99_25455"
     CDS             6332..6724
                     /locus_tag="VFV99_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062755.1"
                     /translation="MIHDFHTLAEHDVSWLAIRQPAVMRLLEAELASDDNDALAAGLE
                     LACRVLGGRTPLGDLRLDHAALAAGMAAVRGGRCDRAMVRSLRDQIDELPCVLMKEEQ
                     DAVATVIAAIIWAVLDASVRDLGDELVA"
     gene            complement(6721..7362)
                     /locus_tag="VFV99_25460"
     CDS             complement(6721..7362)
                     /locus_tag="VFV99_25460"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5062756.1"
                     /translation="MIPILHRDARCVAVDKPSGVATHRGWADDDDALLQLVRDTVDAY
                     VYPIHRLDRGASGVTLFALDKEAARAFSDAWPFADKRYLAITRGHPPEHLMIDHAIPK
                     APGLERVPAKTEIWRKDTFGRYALVEARPHTGRLHQIRRHLKHISCPLIGDVRYGKGE
                     HNRLWRDKLSLHRLALHCSSLRVPHPDGNELVVECALAPDMTDALAAARALFA"
     gene            complement(7359..>9318)
                     /locus_tag="VFV99_25465"
     CDS             complement(7359..>9318)
                     /locus_tag="VFV99_25465"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX5062757.1"
                     /translation="VKTLVSVAEVLGSGDEHLSDAEKARRERSRTATRGIVDIDVSDD
                     GRLVMAPLAGTFYVIDRHSGKRHTIDPKGGAYDPHLSPDGKHIAFVRDGDLWLVGTEP
                     GSAPRQLTKHPEDIEYAVADFAAQEELDRQRGYWWAPDSKQLVFQRTDARKVDTLYVS
                     DARHPEKPPVPFKYPRAGRPNATVDLGIVAIGGGDPKWLTWSLDKYPYLARVTWNARG
                     PLSIVVLSREQQDVALIAFDATGEAKTLVTEHENAWINVPRARGFGYAAGSPLWLADG
                     TFLWTTEAHGDAWTVEHRSATGAVIGQVTKPELGLRQLVGVDGNDLIVEASADPLRSS
                     VWRVPLAGGAPVQISKEDGVAMADFDNGVLVMVTALASGGKHFVAIAGNASHELPSVA
                     EQPKLVPTTALETVTLAGRTHYVAITRPRSFDPNRKYPVLLKVYGGPHAQMVDAARDG
                     YVMDQWYADAGFIVVRSDGRGTPNRGAKWERAISEDLITVPLADQVDALQAIGARYKE
                     LDLTRVGVAGWSFGGYFTAMAILLRPDVFAAGVAGAPVTDWQLYDTAYTERYMRMPEH
                     NVAGYERTSALTHAAKLSRPLLLIHGITDDNVHFANTLSLIEAFYVAGKRAEVITLSS
                     THMVPDPKLNLAKEQVQIDFFRQHLGGQ"
BASE COUNT         1517 a         3076 c         3172 g         1553 t
ORIGIN      
        1 tcgcgcacta tcgcgcggtg atcgcaccga cgcagaaccg catcgatcgc gggctgtggc
       61 agcggctgcg tggactcgcc gagcacaaga aagcaatcgt cgtgatcggg ccgaccacgc
      121 cgacgcgcga cgagctcgat cagccactcg acgagccagc accgaagcgc atcggcaagc
      181 tcaacgaagg ttccctcgac gacatcgtgg gtctcgccga tgatctcgtc gcgctcgcgg
      241 gcgagctctc cgatgactgg catgtcgagc gccccgacga cgtgcgcacg atggcctacg
      301 ccgacgcctc gggtgcgacc cgcgtcgtgt tcgtcgtcag cgatgcggca agacctgtga
      361 ccgcggtcgt gctcgcgagc acagccgtga cgtccgtgcg tgatccactc gccaacgagg
      421 agctaccgat cgatgatggt cgggtgaggg tcgccatggg gccgcgtggc gtccgcatgc
      481 tcgtcgtagg aggatcgtga tgaagaagct agtcagcatc gccgcgctgg tcgcgctcgt
      541 tgcgtgcggc ggtcgtggat tgaacctgac gatgatcgac gcgagcgtcc agcggccgtc
      601 gaacgtcgcg gtctacttca cggtcgacac cgccgatggc gaaccggttc ccgggttgaa
      661 cgtgatggcg ttcaagatct acgaggacgg cgcgccgatc tcggcactcg agagccgaca
      721 gacgatcttg aaccccgagg tatcggcctc gcactacacg ctgctcctca tcgacatgag
      781 cggcagcgtc actggatccg gcgacgttcc ggtgatcgtc gaggcagcga aaggcttcag
      841 ccagcgcgtc gagaagttcc agaaggtctc gatctacgca ttcgacgggt cgccgacgat
      901 tcggcagatc ggcagccccg atcagctgtc gagcttctct gcgaaagatc cgtcgaccaa
      961 tctgaacggc gccatcgtcg agggcctcaa ggtactccac cagcagatga gctcgtcgtc
     1021 gacgccgctg acgtttggca cgctcgtcgt gttcaccgac ggcaccgatc gcgcggggcg
     1081 ggtgacgcgc gacaagctat acgaaacgct cgatcaggcg caggtcgaca tcctcacgat
     1141 cggcgttggc gccgagatcg acggcggtga gctacgcaac atcggccgca atggcgcgat
     1201 cgtgaacaag gaccgcgcgc agatcgcggc gtcgttcgaa gcggccgcgg cacgcgtcga
     1261 ggcgatgtcg aaccgctact acctgcttgg ctactgcagc ccagctcgcg ccggcaagca
     1321 caccgtccgc atcgagacga acgcgaacgg caagagcggc tcgctcgagt acgagttcga
     1381 cgcgaagggc tttggcccga actgcgatcc gaatcgcccg ccgtcgttca acattcggcg
     1441 cccaaaacgc gtcgcggttc agccctagcc ggtctggcga accgggccgg tcggatcttc
     1501 gtcttcgaag ttccgcaccg ggcgcacctc accggcgatg cgatcgacga tcgccacgag
     1561 ctcgctcggc tcgcacggct tcggcaagaa cgcggtggcg ccgatgcgcg cggaaatctc
     1621 cggcgcgtac ggcgacggcg agacgagcac gagcggcggc ccatcgggca gggcgttcac
     1681 gctctttgcg aggtcggtca cgccgactcg cggatcgtac gagtcgatga cgacggcatc
     1741 gatgccgccc tcactgatcc gcagatacgc ttcgcgcagc gtgatcgcga cgagcacttc
     1801 gtgccccgcc tcccgcatcg ccgaccccaa ccggtcgcgc gtatctggat ctgactcgac
     1861 gaggagaata ctgcccacat tgagagacta acccggatcc tcgggaagat gtcagcgcaa
     1921 gatcggtctc cgcctgcatc acttcgcggc agcgccgtcg acagttagga gccggaggtc
     1981 ggcggctgcg atttgcgttt cgcgcgctcg tgcccttcgg cccacccgat ctctttcgtc
     2041 agctgcatgg cgtcctggta gcggttcggt tgcacgaccg cgccgcggga cggcgtctcc
     2101 gtcagtaata gctctgcagt ggcgcgacga tcgccgaggt tgcgcatctc gttgatggca
     2161 gcctgacggt atcgttcggc tttaccgccg agcccggcct tctcgcagag tgcagagagc
     2221 aactgctgga cgcgggctgc gccaaggcga gagcgcactc tttgcgcgtg gaacagtgcg
     2281 gcctcggcga gccgaagcgc gcgatacgaa tcacccgcgg catcgatgag ctgggcctgg
     2341 gcgcggagaa tgcgccagaa ggattcggtg ccctcggtcg cggtgaagcc ctcgcccagg
     2401 gtcgcgaggt cgacgcactc gacgagctcg cggttcgcgg tctcggggtc gccggcgcgc
     2461 tggagggcca ttgcgaggtc caggtacagc tcgcagacga ggttcatgtc gcccgagccg
     2521 atcgcgcggc cgacgccacg ccggagcgcg gcaatcgcac catcgatgtc gccctcggag
     2581 agcgcgatcg cggcacctgc ccgatcgatc atggacacga gcagcggcgc actggaccag
     2641 tcacgcgcct cggcgaggat gccgcgagcg agcgcggtct cgccacgcgt gcgcagaacg
     2701 tccgcaagcc ggaccgacat cgcgacgaac tgctgctcgg ccccgtcctc gatgtcgcct
     2761 gactgtactg cttggcgcac agcgacgagc gcgcgataga agaactgccc ggcgccgtgc
     2821 tcgtcgagct gctcggccgc gtgatcgccg gctgcgcgca gcagatcgac cgcatccttc
     2881 gcgtggcctg cgagatcgtg gtggtggccg agcaaaccga tgtcgtgcga tagcgattgc
     2941 acggcagcgg ccgcgctggc gtggagcgaa cggcgcacgt cggccggcgt cgcgtcgtac
     3001 acgatttcgc ggaccagctt gctggtgaac aacagctcgc ccgacggatg cttgccgagg
     3061 acgccgtgat tctcggcgtc gacgagcgcg ggctcgagcg tctcggtcgg catcatcgag
     3121 cgcagcagat cgagctgggg ctcgataccg agcacggcag cggcttgcag cacgtcgcgg
     3181 gtggactgcg cgagcatcga caggcgcgcg gcgaccaagt cgggaagctt gacgcgcgtg
     3241 tcttcggcgc ggccgccttc gagcaggtac cgcacgacat ggtcgacgtg accgggatac
     3301 gcgcgcgtgg tctcgaacag ctgttgtacc ggcggaaggc cgcgcacgcc ggcggcggcg
     3361 accttcgaga tgatcgcctc gaggtccttg gcgtcgagtg cgccgacctc gagccgcttg
     3421 accgatgccg gccaggcttg gctgaacgcg ggcgtcgctg tcatcacgac gggcggcagt
     3481 gcgaggtcac cgctctcggt ggcgcggcgg aggatctcga ggctcggctc gtcgaagcga
     3541 tcgacgtcct cgcacacgat cgcgaccttc gcgtccaccg cggcgcgttc gatcgcgcgc
     3601 agcgccgacc acaccatctc gcggcggcgt acgggtgctt cgagctcgag cagcgttgtc
     3661 ggttgaccga acagctgggc gatacccggc acgtcacgct cgttgagccc gaccgccagc
     3721 acggcctcgc gcagctccat ctcgctcgac agcgcaggca gctgcaggat cgcggcgagc
     3781 agcgaacgga tcggatagaa cggcgccgcg aggccgctcg gatccggacc gatctgatag
     3841 acgacgcggc tatcggcgag cagctcggga tagaccttgc gtaacagcgt cgagcgaccg
     3901 gcgccttcga ggccgacgag caacaggccg ccgttcgagc cgggtgcgcg cttcatgtgg
     3961 tcgagcagct gctgcttctc gaggacgcgt ccgcagaacg gcagcggcag cgcatcgctc
     4021 ggggcgggcg tggcggcggg aacctcgaac gtcttcgaga tccggatccg cggcgcgccg
     4081 cactcggggc agaacttgaa cgtcggcgtg accgacgcgc cacacgattc gcacttatcg
     4141 gtgaccggca gcgcacgcat gcgcgcgcga atgccgagca gcgcctcgcg catctcgtgg
     4201 gctgacgagt agcggtcgct cggatgcttg gccagcgcct tgccgatcac cgcgtcgatc
     4261 tcgcgcggca cgccttcgcg gcgcgtcgtc agcagcgtcg gcgccacctt caagtgattc
     4321 gtcaggatct ccgtcgtcga gctcgcgaag aacggcgtct tctcggcgag cagctcgtag
     4381 aggatgatgc cgactgcgta gatatccgac gcgaacgacg gcggcgcgcc gctgatcact
     4441 tcgggcgcca tgtactcggg cgtacccgag atcgagcggt cctccgcttc gcgcacgccg
     4501 gtgacgaggc gcgcgatacc gaagtcgacg atcttgacga gatcgatacc ggcgcggcgc
     4561 tggtcgacga tgatgttgtc gctcttgagg tcggcgtgga cgacgccggc aagatgcgct
     4621 tcctcgatgc cagcaagcga ctggcacacg acgtcgatca cgcgctcgac accgagcgga
     4681 tgttcgttga cgagcagctg cgtgagcgtc ggtcctttga cgtactccat cgcgagatag
     4741 agcaagccgt cgggcgcttg gccgtagtcg ataatcgaga cgcagttggg atggttgagg
     4801 cgcgaagcgc tcatcgcttc gccacggaac cgcgtgatca tccgcgggtc cgcgcagagc
     4861 tcttcgttca ggatcttgac cgcgaccgtg cggccgagcg ccatctgatc ggccttgtac
     4921 acggcaccgc tgccgccaat gccgatgcag gccgtcaact tgaacttgtc gcccagtacc
     4981 cggccgacaa gcttcagtga cttttgtact tggtcctgaa cactcaccga cgtgccgtcc
     5041 cttaccgttc ttgcgagctt ctcacgaagt tcgctcgatt acgacggcga tcccctggcc
     5101 gacgccgata cacatggatg ccagaccata ctggccggac cgatgtgaaa gctcggtcat
     5161 cagagtaagt acgagtcgtg ccccggaggc tccgagcggg tgcccccacg cgatcgcacc
     5221 accgttcaca ttcacccgct ccgggtcgag cccgatctgg tcgatacagg ggatggcttg
     5281 ggcggcgaac gcctcgttta gctcggcaag atcgatgcgc tgcggcgtta ctcccgccag
     5341 cgtgagcgcc tttttactcg cggggatagg accgagtccc atgtagctga cctcgacgcc
     5401 ggccgccgcg cccgcgacgt accgcgcctt cggggcaaga cccagctcct tcatggcggg
     5461 ttcgctggcg agcaagagcg cggccgcgcc gtcgttgagc cctgacgcgt tgcccgcggt
     5521 gacggaccca ttcttcgcaa aagccggacg aagcttctgc aactgctcga tcgttgtctc
     5581 ggggcgcggg tgctcgtcca tagtcacttt ggtttcggcg cccgtcttct tgtcgacgcc
     5641 ggtgacgatc ggcgtgatct cttcggcgat ccggccactc gcgatggcct tgccggcgcg
     5701 ctgctgtgac aacagtgcga accgatcctg tgcttcgcgc gacacgttgc agcgctcggc
     5761 gactttctcg gcggtctcgc ccatcgcgag cgtgccgtgc atctgctcca tgcgcggatt
     5821 gatgaagcgc cagccgagcg tcgtgtcgta gatcgtctgg ttgccgcgcg ggaacgcgtc
     5881 ggtcggcttc gccatcacct gcggcgcgcg cgacatctgc tcgacaccgc ctgcgatcac
     5941 gaggtcggcc tcgccggtca tgatcaaacg cgctgcatcg gcgattgcct gcatgcccga
     6001 gccgcagagt cggttgaccg tgatgccagg aaccgagacc ggcagccctg cgagcagtgc
     6061 ggccatgcgc gcgacgttgc ggttgtcttc gccggcctgg ttcgcacagc cgaggatgac
     6121 gtcgtcgatc cgctcggtgg gaatcttcgt gcgggcaacg agggcagcga tcacggccgc
     6181 ggcaagatcg tccgcacgga cacccccgag gccgccgcca taccgcccga atggggagcg
     6241 aataccgtcg acgatgaaag ctgcgcgagt catgcgcgca ccatacgccg aaaactggcg
     6301 aacgggagcc gaggtcccta ccgtcgaggc catgatccac gacttccaca ccctggccga
     6361 gcacgacgtg agctggctcg cgatccgcca gcccgcggtg atgcgattgc tcgaggcgga
     6421 gctcgcgtcg gacgataacg atgcgctcgc cgccggtctc gagctcgcat gccgcgtcct
     6481 cggcggtcgg acacccctcg gtgatcttcg gctcgatcat gcagcgctcg ccgccggcat
     6541 ggctgcagtg cgcggcggtc gctgcgatcg cgcgatggtg cggtcgctgc gcgatcagat
     6601 cgacgagctg ccgtgcgtgc tcatgaagga agagcaggac gcggtcgcga ccgtgatcgc
     6661 tgcgatcatc tgggccgtac tcgatgccag cgtgcgcgac ctcggcgacg agctcgtcgc
     6721 ctaggcgaac agcgctcttg ccgcggcaag cgcgtcggtc atgtccggcg cgagcgcgca
     6781 ttcgacgacc agctcgttgc cgtcggggtg agggacgcgt agcgagctgc agtgaagcgc
     6841 gagccggtgc agcgagagct tgtcgcgcca cagccggttg tgttcgccct tgccatagcg
     6901 cacgtcgccg atcagcggac acgagatgtg cttcaagtgg cggcggatct ggtggaggcg
     6961 gcccgtgtgc ggccgcgcct cgacgagtgc gtagcggccg aacgtgtcct tgcgccagat
     7021 ctcggtcttc gccggcacgc gctcgaggcc gggcgctttc gggatcgcgt gatcgatcat
     7081 caggtgctcg ggaggatggc cgcgcgtgat cgcgaggtag cgcttgtccg cgaagggcca
     7141 ggcatcggag aacgcacgtg cggcctcctt gtcgagcgcg aacagcgtga cgccggaggc
     7201 gccgcgatcg aggcgatgga tcggatacac gtacgcgtcg acggtgtcgc gcacgagctg
     7261 gagcagcgca tcgtcgtcgt cggcccaacc gcggtgcgtg gcgacaccac ttggtttgtc
     7321 cacggcgacg cagcgcgcgt cgcgatggag gatgggaatc actggccgcc gaggtgctgg
     7381 cggaagaagt cgatctgcac ctgctccttc gcgaggttca gcttcggatc cggaaccatg
     7441 tgcgtcgatg acagcgtgat cacttcggcg cgcttgcccg cgacgtaaaa cgcttcgatc
     7501 agcgagagcg tgttcgcgaa gtgcacgttg tcgtcggtga tgccgtggat gagcagcagc
     7561 ggccgcgaca gcttcgcggc gtgcgtgagc gcgctcgtgc gctcgtagcc agcgacgttg
     7621 tgctcgggca ttcgcatgta ccgctcggtg tacgcggtgt cgtagagctg ccagtcggtg
     7681 accggcgcac cggcgacgcc ggcggcaaac acgtcggggc ggagcagaat cgccatcgcg
     7741 gtgaagtagc cgccgaacga ccagccggcg acgccgacgc gcgtcaggtc gagctccttg
     7801 taacgagcgc cgatcgcttg cagtgcatcg acctgatcgg cgagcggcac cgtgatgagg
     7861 tcttcgctga tcgcgcgctc ccacttcgcg ccgcggttcg gcgtgccgcg accatcgctg
     7921 cgcacgacga tgaagcctgc atccgcgtac cactgatcca tcacgtagcc atcgcgtgca
     7981 gcgtcgacca tctgcgcgtg cggaccgccg tacaccttga gcagcacggg atacttgcgg
     8041 ttcggatcga agctgcgcgg ccgcgtgatc gcgacgtaat gcgtgcgccc agcgagcgtc
     8101 acggtctcga gcgcagtcgt cggcacgagc ttcggctgct cggcgaccga cggcagctcg
     8161 tgcgaggcgt tgccggcgat ggcgacgaag tgcttgccgc ccgacgcgag cgccgtgacc
     8221 atcacgagca cgccattgtc gaagtcggcc atcgcaacgc cgtcttcctt gctgatctgg
     8281 accggtgcac caccggcgag cggcacgcgc cacacgctgg agcgcagcgg atccgccgag
     8341 gcctcgacga tgaggtcgtt gccgtcgacg ccgacgagct gacgtaggcc gagctcgggc
     8401 ttcgtgacct gaccgatcac cgcgccggtc gcgctgcgat gctcgacggt ccacgcgtcg
     8461 ccgtgcgcct cggtcgtcca cagaaatgtt ccgtcggcga gccacagcgg tgagccggcc
     8521 gcgtagccaa agccacgcgc gcgaggcacg ttgatccatg cgttctcgtg ctcggtgacc
     8581 agcgtcttcg cttcacccgt cgcgtcgaac gcgatcagcg cgacgtcctg ctgctcgcgc
     8641 gacagcacga cgatcgacag cggaccgcgc gcattccacg tcacgcgtgc gaggtacgga
     8701 tacttgtcga gcgaccacgt cagccatttc ggatcgccgc cgccgatggc gacgatgccg
     8761 agatcgacgg ttgcgttcgg ccgccccgcg cgcgggtact tgaacggcac gggcggcttc
     8821 tcggggtggc gcgcgtcgct gacgtacagc gtgtcgacct tgcgtgcgtc ggtgcgctgg
     8881 aacacgagct gcttgctgtc gggcgcccac cagtagccgc gctggcgatc gagctcttct
     8941 tgcgccgcga agtcggcgac cgcgtactcg atgtcttcgg gatgcttcgt gagctggcgc
     9001 ggtgcgctgc ccggctcggt cccgacgagc cagaggtcac cgtcgcgcac gaacgcgatg
     9061 tgcttgccgt cgggcgacag gtgcggatcg taggcgccgc ccttcggatc gatcgtatgg
     9121 cgtttgccgg agtggcgatc gatcacgtag aacgtgcccg cgagcggcgc catcacgagg
     9181 cggccgtcgt cgctgacgtc gatgtcgacg atgccgcgcg ttgccgtgcg actgcgttcg
     9241 cggcgtgctt tctccgcatc cgaaaggtgc tcgtcaccgg agccgagcac ctcggcgacc
     9301 gagaccagcg tcttgacc
//
LOCUS       DASUFX010000632         5675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2047170, whole genome shotgun sequence.
ACCESSION   DASUFX010000632 DASUFX010000000
VERSION     DASUFX010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047170"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFV99_25470"
     CDS             <1..840
                     /locus_tag="VFV99_25470"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEX5062758.1"
                     /translation="KKGWSFMGAAFAMARENKKLLAPSLIQVLVSIMYWVAWIAVLIA
                     IHPHWSNGTWAGVGAIATFGSFLIFYFFCGMTVNMIDVHLKGGKPSIGDGARDAGKNF
                     VAIVFLALVSTVIEMFARAARDNDSIVGKIVAGIVEAIWTTLAFLLLPAIIIEDAGFG
                     AAMKRVRALHKGNLLLIGIGEVGVRGVTGLIGFLWMMLVVGVIWVDVQVFSGWTALIF
                     GVVVGGTMISLFVAFSTYIRMAYYTCLYLWAADVEKQGQSAPAPLPLAIALGHREAPR
                     RAA"
     gene            complement(849..1520)
                     /locus_tag="VFV99_25475"
     CDS             complement(849..1520)
                     /locus_tag="VFV99_25475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5062759.1"
                     /translation="MAVGCAEGLDGDDPEVGTDQGVDYDQDNPNAPLDDGKSDLPRYM
                     IPTDLPMLVAPEIIVSLDGLTVHLFDRATGFQAIYPAGVGMKNSRGVSITPTGHFQSG
                     ANTTDPWWYVQRRTVPDYFGGFPFLRTTARNSDGGNTYALHGPITEQLIRGYVSHGCV
                     RMRGNDIVRLFWMVKPHASTPITIQKEVELDRNGKKVDVGTTPALYLPGEAIRFGASV
                     GPRPF"
     gene            complement(1723..2847)
                     /locus_tag="VFV99_25480"
     CDS             complement(1723..2847)
                     /locus_tag="VFV99_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5062760.1"
                     /translation="MSATEQAATVSSYVTGTCSTSVVLGLSKQISDEIGCMSPTALVR
                     FTPSTKIQVTSSAVLPYLGKSAKTALENVTATVQINSAFRTIAQQYLLVKWHDAGRCG
                     ISAAAAVGRSNHESGRALDLANYSSVRSSMTSKGWTWLGSSDPVHFDYLGAADIRGKD
                     TLAFQRLWNRNNPNDRISEDGAYGPQTEARLRQSPSTGFAQGATCIAASEYQADVMQV
                     DGPDRMQPQARAHFRLMIQNNSDTEWAGTTELRLATAAFSKLHDDSWLGNSVITTLGQ
                     PVPAHTMGEISFDVTTPAVTEETPIFEELVLADGGTQHGGVQLALTVVPDMEEPTSGD
                     ADDQHDDGYDQQVSGGCAAGGGSLGFGAIALLLAARRRRR"
     gene            3109..3735
                     /locus_tag="VFV99_25485"
     CDS             3109..3735
                     /locus_tag="VFV99_25485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062761.1"
                     /translation="MMRSGWMLAVLANLAMTGCDYVFRIDHVDAVHDAPPNALDARRD
                     SQMLELDAPEACPDNYMRVDETPATSFYRWVTAPTTWDLAEFDCENDSVTDITHLAVL
                     DDVGELAGVRTWIGTQTSFSFISLLGYARDTGSDPLQFYAVTGAPVAKTQPPWNLNEP
                     DNGGSEGGEETIVWFSHQDNLIDGPWTYSQLYVCECDHQRVTKQFTLH"
     gene            complement(3732..4508)
                     /locus_tag="VFV99_25490"
     CDS             complement(3732..4508)
                     /locus_tag="VFV99_25490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5062762.1"
                     /translation="MPVHLREVIGYLDTTLEIDRFKDYAPNGLQVEGALEIERIVTGV
                     SASAELIQRAIDVGADLIVVHHGLVWGPGLAKIAGPLARRLKLLLANDISLAAYHLPL
                     DKHVRLGNNTGLCDALALGATREAFGEVRGAALGLAGTWSPPLAKQEAINRVAGGVCK
                     GQPPRFVFPYGPEQVRKVGVCSGAASDLLEAAAAIGCDMFVTGELAERAGDLAKELQI
                     TLVAAGHVATEVFGAMRVADELRMRFPSVETQFVFVPNPL"
     gene            4534..5313
                     /locus_tag="VFV99_25495"
     CDS             4534..5313
                     /locus_tag="VFV99_25495"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX5062763.1"
                     /translation="MLDVASAVVRPGVGRSDVHRARGAGPLRLLCPRAAGDAAWIVTS
                     SLGGGLVDGDAVALEVSVDAGATAVVTTQASTKVYKGSSQQRTHVRVAGDGTVLVVPD
                     PVVPYRAASFAQVTEVELARDSTLILCDVLTAGRVAYGERWSATRLDSTLRLSIDGVC
                     VLHDRIVLAGDAAAHMKRFTALATAMLVGPRVRDLAAVRLAALGTVERGAPVIVAGSP
                     LADGALFRIAGEDVESVVKVTRDLLRDACSRVGEDPWSRKW"
     gene            5327..>5675
                     /gene="ureA"
                     /locus_tag="VFV99_25500"
     CDS             5327..>5675
                     /gene="ureA"
                     /locus_tag="VFV99_25500"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX5062764.1"
                     /translation="MRLAPRDVDKLLLHNAGFLAQKRLARGVRLNYPEAVALIATQLL
                     ELIRDGRSVAELMDIGRTILGRYQVQDGVPELVAEVQVEGTFPDGTKLVTVHHPIVAD
                     HGDLELALYGSFLP"
BASE COUNT          922 a         1869 c         1892 g          992 t
ORIGIN      
        1 aaaaagggtt ggtcgttcat gggcgccgcg tttgcgatgg cgcgcgagaa caagaagctg
       61 ctggcaccgt cgctgatcca ggtgctcgtc agcatcatgt actgggtcgc gtggatcgcg
      121 gtgctcatcg cgatccatcc gcactggtcg aacggcacgt gggccggcgt cggtgcgatc
      181 gcgacgtttg gctcgttcct gatcttctat ttcttctgcg gcatgacggt gaacatgatc
      241 gatgtccacc tcaagggcgg caagccgagc atcggcgacg gcgcgcgtga cgcgggcaag
      301 aacttcgtcg cgatcgtgtt cctcgcgctc gtgtcgacgg tcatcgaaat gtttgcgcgc
      361 gcggcgcgcg ataacgactc gatcgtcggc aagatcgtcg ccggcatcgt cgaggcgatc
      421 tggacgacgc tcgcgttcct gctgctgcca gcgatcatca tcgaggacgc cggctttggc
      481 gccgcaatga agcgcgtgcg cgcgttgcac aagggcaact tgctcttgat cggcatcggc
      541 gaagtcggcg tccgcggcgt caccggcctg atcggcttcc tctggatgat gctcgtggtc
      601 ggcgtgatct gggtcgacgt gcaggtgttc agcggctgga ccgcgctgat cttcggcgtc
      661 gtcgtcggcg gcacgatgat ctcgctgttc gtcgcgttct cgacgtacat ccgcatggcc
      721 tattacacgt gcctgtacct gtgggccgcg gacgtcgaga agcagggcca gtcggcgcct
      781 gccccgctgc cgctcgcgat cgcgcttggc caccgcgaag cgccgcgtcg cgccgcatag
      841 tccggatcct agaacgggcg cggaccgacg gacgcgccga accggatcgc ttcgccgggc
      901 agatacagcg ccggcgttgt accgacgtct accttcttgc cgttgcggtc gagctcgact
      961 tctttctgga tcgtgatcgg cgtgctcgcg tgcggcttga ccatccagaa cagccgcacg
     1021 atgtcgttgc cgcgcatgcg cacgcagccg tgcgagacgt agccacggat cagctgctcg
     1081 gtgatcggac cgtgcagcgc gtacgtgttg ccgccgtcgc tgttacgcgc ggtcgtgcgg
     1141 aggaacggga agccaccgaa atagtccggc accgtgcgac gctgcacgta ccaccacggg
     1201 tcggtcgtgt tcgcgccact ctggaagtga ccggtcggcg tgatgctgac gccgcgcgaa
     1261 ttcttcatgc cgacgccggc gggatagatc gcctggaagc cggtcgcgcg gtcgaacagg
     1321 tgaacggtga gaccgtcgag cgagacgatg atctcgggcg cgaccagcat cggcaggtcg
     1381 gtcggaatca tgtagcgcgg caggtcgctc ttgccgtcgt cgagcggcgc gttcgggttg
     1441 tcctggtcgt aatcgacgcc ctggtcggtt ccgacttcgg gatcatcgcc gtcgagacct
     1501 tcggcgcagc caaccgccaa cgcgaccaga gagataagcg aagcgagacg cagcatgtgc
     1561 cgacgccgcc tcagcacgcg ctgtgccgag agcccactca atggattcgt ggacttcaca
     1621 cacgaatgat ctggtcactc tcgtcccctg gatgctcgag tgaccagaat catttcggcc
     1681 agctacacac gaagtgcaga aaaggatggg ggcagacccc atttagcggc ggcgacggcg
     1741 tgccgcgagc aagagcgcga tcgccccgaa gccaagcgag ccgccaccgg ctgcgcagcc
     1801 accactgacc tgctggtcgt aaccgtcgtc gtgctggtcg tccgcatcgc cgctcgtcgg
     1861 ctcctccata tcgggcacga ccgtcagcgc gagctgaacg ccaccgtgct gcgtaccgcc
     1921 gtcggccagc acgagctctt cgaagatcgg cgtctcttcg gtcacggccg gcgtcgtgac
     1981 gtcgaagctg atctcgccca tcgtgtgcgc gggcaccggc tggccgagcg tcgtgatcac
     2041 gctgttgccg agccagctgt cgtcgtggag cttgctgaac gctgccgtcg cgaggcgcag
     2101 ctcggtcgtg ccagcccact cggtgtcgct gttgttctgg atcatgagcc ggaagtgcgc
     2161 gcgcgcctgc ggctgcatgc gatccggacc gtcgacctgc atgacgtcag cctggtattc
     2221 cgacgccgcg atgcaggtcg cgccctgtgc gaagccggtc gacggcgatt ggcgaaggcg
     2281 cgcttcggtc tgcggaccgt acgcaccgtc ctccgagatc cggtcgttcg ggttgttgcg
     2341 gttccacagc cgctggaacg cgagcgtgtc cttgccgcga atgtcggcgg cgccgaggta
     2401 atcgaagtga accggatcgc tcgagccgag ccaggtccag cccttcgagg tcatcgacga
     2461 acgcacgctc gaatagttcg cgagatcgag tgcgcgaccg ctctcgtggt tcgagcgtcc
     2521 caccgcagcg gccgccgaga tgccgcagcg gccggcgtcg tgccacttca cgagcaagta
     2581 ctgctgcgcg atcgtgcgga acgcgctgtt gatctgcacc gtcgccgtca cgttctcgag
     2641 cgccgtcttc gcgctcttgc cgaggtacgg cagcaccgcg ctgctcgtca cctggatctt
     2701 cgtcgatggc gtgaaccgca cgagcgccgt cgggctcatg cagccgatct catcggagat
     2761 ctgcttcgac aagccgagca cgacggaggt cgagcaggtg ccagtgacgt acgacgatac
     2821 ggttgctgct tgctcggtcg cgctcaccta ggggccatct gcaagacaac cagtgctcgc
     2881 aatgagcgca atggggagca gaccccgcac cacggagttt gcggccccaa tgtaggtgtc
     2941 ggcgaaagat ttgatattcg acgcgcggcg aagacgatcg agcatggtgc cgctgctctc
     3001 agcaacgacc gagccgaaaa ccgcgaattt ctcgacgcat ttcgcgcaaa cgcatggtgc
     3061 gcgcgtccct gcacttcacg tgcaaatcga cgcacgttag tctgaacgat gatgcgttcg
     3121 gggtggatgc tcgccgtgct cgcgaatctc gcgatgaccg gctgcgacta cgtgtttcgc
     3181 atcgatcacg tcgatgccgt gcacgacgcg ccaccgaacg ccctcgatgc gcgccgcgac
     3241 tcgcagatgc tcgagctcga cgcgcccgaa gcatgccccg acaactacat gcgggtggac
     3301 gagacgccgg cgacgagttt ctaccgctgg gtcacagcgc cgacgacctg ggacctcgcc
     3361 gagttcgatt gcgagaacga ttcggtcacc gacatcacgc atctcgctgt gctcgacgat
     3421 gtcggcgagc tggcgggcgt gcgcacgtgg atcggcacgc agacctcgtt cagcttcatc
     3481 tcgctgctcg gctacgcacg tgacaccggc tcggacccgc tgcagttcta cgcggtgacc
     3541 ggagcaccgg tggcaaagac gcagccaccg tggaacctga acgagcccga caacggtggc
     3601 tccgagggcg gcgaagagac gatcgtgtgg ttctcgcacc aggacaatct gatcgacggc
     3661 ccgtggacgt actcgcagtt gtacgtctgc gagtgcgatc accaacgagt cacgaagcag
     3721 ttcacgctcc actagagcgg attcggcacg aacacgaact gcgtctcgac gctcgggaac
     3781 cgcatgcgta gctcgtccgc gacgcgcatc gcgccgaaca cctcggtcgc aacatggccc
     3841 gcagcgacga gcgtgatctg cagctccttc gcgagatcgc ccgcgcgctc ggcaagctcg
     3901 ccggtgacga acatgtcgca accgatggcg gcggcggctt cgagtaaatc gctcgctgca
     3961 cccgagcaca cgccgacctt gcgcacttgc tcgggtccgt acggaaacac gaaccgcggc
     4021 ggctgccctt tgcacacgcc gccggcgaca cgattgatgg cttcttgctt cgcgagcggc
     4081 ggcgaccacg tgccggcgag accgagtgcg gcgccgcgca cctcgccgaa ggcctcgcga
     4141 gtcgcgccga gcgcgagtgc atcgcagagg cccgtgttgt tgccgagtcg cacgtgcttg
     4201 tcgagcggca ggtgataggc ggcgagcgag atgtcgttcg cgagcagcag cttcaagcga
     4261 cgcgcgagtg gccccgcgat cttcgcaagc cccggtcccc agacgagacc gtgatggacg
     4321 acgatcagat cggcaccgac gtcgatcgcg cgctggatca actcggcgga cgccgacacg
     4381 ccggtgacga tgcgttcgat ctcgagcgcc ccttcgacct gcaacccgtt cggcgcgtag
     4441 tccttgaagc gatcgatctc gagcgtcgtg tccaggtatc cgatgacctc gcgcaggtgc
     4501 acaggcatac ggggcaagcg tacagtagcc gccatgctcg atgttgcgtc cgcggtcgtt
     4561 cgccccggtg tcgggcgtag cgatgtccac cgcgcccgcg gcgccgggcc gcttcgcctg
     4621 ctctgccctc gtgcggcggg cgacgccgcg tggatcgtga cgagcagcct cggcggtggg
     4681 ctcgtcgacg gtgacgcggt cgcgctcgag gtcagcgtcg atgcgggtgc gaccgccgtc
     4741 gtgaccacgc aggcctcgac gaaggtttac aagggaagct cgcagcagcg cacgcacgtg
     4801 cgggtcgccg gcgacggcac cgtgctcgtc gtgcccgatc cggtcgtgcc gtatcgggcg
     4861 gcgagcttcg cgcaggtcac ggaggtcgag ctcgcgcgtg actcgacgct gatcctgtgc
     4921 gacgtgctca ccgctggccg tgtcgcctac ggcgagcgct ggtcggcgac gcgactggat
     4981 tcgacgctgc ggctgtcgat cgacggcgta tgcgtgctgc acgatcgcat cgtgctcgcc
     5041 ggcgacgccg cggcgcacat gaagcggttc accgcgctcg ccaccgcgat gctcgtcggc
     5101 ccgcgcgtgc gcgacctcgc ggcagtacgg ctcgcagcgc tcggcaccgt cgagcgtggc
     5161 gcaccggtga tcgtcgccgg ctcgccgctc gcagatggcg cgctgttccg gatcgcgggc
     5221 gaggatgtcg agtcggtcgt gaaggtaact cgcgatctgc tgcgtgacgc ctgctcccgc
     5281 gtcggcgagg acccgtggag ccgcaagtgg tgatacgacc ggcggtgtga ggctggcacc
     5341 acgtgatgtc gacaagctgc tgctccacaa cgcgggattt ctggcccaga agcgcctcgc
     5401 gcgcggcgtg cgcctgaact accccgaagc cgtcgcgctg atcgcgaccc agctgctcga
     5461 gctgatccgc gatggccgct ccgtcgccga gctgatggac atcgggcgca cgatccttgg
     5521 ccgctatcag gtacaagacg gcgtgcccga gctcgtcgcc gaggtgcaag tcgagggcac
     5581 gttccccgac ggcaccaagc tcgtcaccgt tcatcacccg atcgttgccg accacggcga
     5641 tctcgagctc gcgctctacg gcagcttctt gccag
//
LOCUS       DASUFX010000633        13805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_519281,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000633 DASUFX010000000
VERSION     DASUFX010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519281"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..527
                     /locus_tag="VFV99_25505"
     CDS             162..527
                     /locus_tag="VFV99_25505"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5062765.1"
                     /translation="MNCPICKRPLVQAGRTWKCETDDGAWVRSDVLVPMLEQAASTLV
                     ELPWQANQEDHVRNCPECSHDMQTVKLGTVALDRCVEHGVWFDRKELAAVLKQAKSFK
                     ATDKHEHEGILRTLQKLFS"
     gene            complement(564..2579)
                     /gene="fusA"
                     /locus_tag="VFV99_25510"
     CDS             complement(564..2579)
                     /gene="fusA"
                     /locus_tag="VFV99_25510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5062766.1"
                     /translation="MFPLERIRNIGIVAHVDAGKTTLTERVLLYTGVIHDPGEVHDGT
                     AHTDFDEIEQRRGITINSAAVTADWQGHRIHLIDTPGHVDFTIEVERSLRVLDGAVVV
                     LDGVAGVEPQTETVWRQANRHRVPRIVFVNKLDRVGADYARSLAELHAKFGVRAVAVT
                     WPLVEDNVLVGVVDLVERTAIRWSDTRGWKFTSSPVTLSDNLVIARERLLEAAADEDP
                     EILAALVEGKPEGYTIDAARIRAALRRGTIAGRLVPVLAGSAYKYRGVQPLLDAVVAY
                     LPSPGDREPVKSIDGTQTRAPMRDAPLAALGFKVGFDDHGQMTFVRVYSGVLARGDHV
                     VTGRGKKLRVGRLVQLVADERVEVDQLAAGEIGAILAAPLANGETLTDPARPIVLEAI
                     TAPEPVMRVSIEAKNAAEREKLSLVLSRMIAADPSLRIETDRETGESLLAGMGQLHLD
                     IAIERLEKEHNLVVATGQPKVAYRSTITRTARHELRYAKQNSGPGQFAQVLIEVGPAA
                     PGSGLVFEDRIKGGAIPKPFIKAVEAGVRGAMADGLLGGHPVVDVRVVLLDGAIHERD
                     SNEPAFRIAGSLAFKAAAAEAGPIMLEPIMLLEVTCPEDEVGAVIGDIGRRRGEVLAL
                     DSRGADRVVRAEVPLAETFGYASVLGGLTHGRGHFTLEPTRYAPVTK"
     gene            complement(2940..4151)
                     /locus_tag="VFV99_25515"
     CDS             complement(2940..4151)
                     /locus_tag="VFV99_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062767.1"
                     /translation="MGEARGNSEVRREVTLYNEGMRLIVVGVLVALASSAHADRIVDR
                     CEPLAPRTLPVDGAKNVPLNAKIWQLGTKEGFFDYDVGSVTDRRTITVQSQPFDRNED
                     HPVVTYPLRDLQPSLLYWFHGTGTTISFETDSSIDTTPPDLPTVDEVQIELEYQPLER
                     MPVARLRLASHVTADTALLQIAVTDRSGTQTVTTTPDHTDICRPGLRLTPGRVRVRVF
                     ALDLAGNRSASDDGQPFVVDVADPTAKDPQGHVGADLGNVSLILFGPVLLAAILLGIL
                     LVISLRHWRVQRGEGEPVSQLVADHIARAVFRRASSGSVAAAAGVVSAFVLHNSLLVI
                     VVALIACVPLSAFIASRRVLRELGRDRARAELRETVLVVYSPNGQARLATSPRVIDKA
                     RAHAVPTSVAR"
     gene            complement(4109..5155)
                     /locus_tag="VFV99_25520"
     CDS             complement(4109..5155)
                     /locus_tag="VFV99_25520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062768.1"
                     /translation="MRQRLALAVLMVIAGHAEAKRITCSCGNEIALPEWGAQDLPINT
                     KVMRILENGAYLYQLRPELTANVLYADEATGVSFTPNDKRDDAPPEAPHDVNAYVATG
                     LVKNQQTLSLFGNYAPDTAVIHVELRQADRFVTLYTTPNRLYLCRSGMDLGPGTVDVR
                     ITAIDIAGNESAPFATSVKPSPIDDVEDHCRDSGFYEHHHHDHGFEILFLLLLYPLCV
                     VAWFLIVIVRRATVKRTPAEPVAHLEAAEVVRRLLRWQIVWAVMTIAGVLGLVHARSE
                     LPIFLAPFPIVAVAKLYWIRRAQVLLDQPETDAVRHGRWLCVTSLRDTVTVRASDIDF
                     VWAKRAGIPKSVAK"
     gene            complement(5157..6887)
                     /locus_tag="VFV99_25525"
     CDS             complement(5157..6887)
                     /locus_tag="VFV99_25525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062769.1"
                     /translation="MSRDSLEDAAAAHLDGAPHLRLSVTKALAMHLADTVWAAAERHV
                     FGDAQGKRSGSPRIGRWYDFKSLPGRARSHTKKRKWETFRLHGTLAGHRVAYTDRGGD
                     FVQPRRLRAVELTTKTWWSYTGPLAIVFSGLGDGTLVLPVRLPTAPSNQPILDYHLTD
                     PSKWHKIDLVRHRDPNAEGGWRYEAHLMVLTTPYVSPSTAARRAKVAIESVDRTAGID
                     VNVSCLAIASHDTGTAMRLTRVERTGEQKQRDRGRKRRERRRQRDLERSRRAANRAQY
                     HLSKRQEKRARRRAARNLAPVDVIPMGPRKARSDGVPLQSYRHDQLSASFRRTRAAQV
                     AEAKAGARARRDHAREVAAGVVATHGYQLVVEDCTIAAWASSWGSAVAAFTPGMLVAA
                     LDREARAVAAIAGGEGGVARATTRTTALSQHCPCGARVPKRLADRVHRCAACSLVGDR
                     DDVAAVLASFVVHVQRGEPASACVDYAASTDALIEIRRVLGSSYHGWQDTLSESNDLS
                     ARDGSFIAWTTSTSDSVAMARRNVGTVSCSTLNDRRPSDHVRTSTRANRQVYEVRSSM
                     DVLPGLLLDS"
     gene            7408..8154
                     /gene="pyrH"
                     /locus_tag="VFV99_25530"
     CDS             7408..8154
                     /gene="pyrH"
                     /locus_tag="VFV99_25530"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5062770.1"
                     /translation="MAESRKPAYKRVLLKISGEALMGNASYGINPEVLRVVAAEIAET
                     AKLGVEIAVVVGGGNIWRGAQQGSGMDRASADYVGMLATVMNAVSLQSAVEKSGVTTR
                     VQTAIPISQIAEPYIRRRAIRHLEKGRVVIFGAGTGNPFFTTDTAAALRAIEIGADVL
                     LMAKNIDGVYDKDPKKHKDARRYSAVTFNDALVHDLRVMDGTAFALCRDNDLSIAVFE
                     LATPGNIRRVVCGEPIGTIVKNNQETRFAN"
     gene            8172..8729
                     /gene="frr"
                     /locus_tag="VFV99_25535"
     CDS             8172..8729
                     /gene="frr"
                     /locus_tag="VFV99_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5062771.1"
                     /translation="MINDITTDAEDGMKKAVDSFKRDLQKIRTGRANTSMLDGIKVDY
                     YGTPTPVNQVATVQVVDARLITVKPWEKNMIAVIDKTIRASDLGINPVADSELVRLPI
                     PPLTQERRKELAKVVGKQTEEARVAVRSARRDAMDMIKDAEKDKQITEDERKKGETKV
                     QELTDKYIGSIEDIAKAKEKEIMEL"
     gene            8848..10845
                     /locus_tag="VFV99_25540"
     CDS             8848..10845
                     /locus_tag="VFV99_25540"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062772.1"
                     /translation="MSGIPAGAGSLIGKRLGKYEILALLALGGTAEIYLARIGGAAGF
                     EKYVVVKCLHDHLADDTDFVKMFLDEARMAAMLDHSNIVQTFELGEYTNRYYMVMEFL
                     AGLSLAMVVRRAQERLQGGMLPVPLILNVAAQACAGLHYAHERSASGKPLNMVHRDIS
                     PQNLVISFEGVVKVVDFGIARAELRETKTKAGTIKGKFAYMSPEQCVAANVDRRTDVF
                     ALGVIVHELLVGRRLFKRNSPYETYQAVIECAVEPPSRINNTLDPALDEVVMRAVAKD
                     KDKRYPTAEAFGDAMLGYLHHRGKASGGGELGRFFEQYFAQEIEEHGERMRELIAGRP
                     ASVEGASGWDDADEQANQTVDLSRRKAEEQSISLATGEIMELNTGDASLDAFVGDLEE
                     ENDPDALPADKTNIVHNPLEMIEQLNAQVARNTGKTPVPPGSITASGIAPLPAPPTGP
                     KGAVGKPGSGRFPDMPNMPTQLAPETKLEHAPTAIGAAVNPMGHTQAEMQAQPAHFDP
                     NVAPAGPMSSTHYAVRPSQMQGAQQPNLGATAPAPALQGHPHGPMQGHASAPMPAHAP
                     VLNDYPVMPGGQRPFSSDLAPSNLSSSPQQRPSYGNAPLDPQAAANLMSPVGGGYPQT
                     DWERAAGVSAKALPTWLLLALFGGALLGALLLTLMIAKIFS"
     gene            10880..11248
                     /locus_tag="VFV99_25545"
     CDS             10880..11248
                     /locus_tag="VFV99_25545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5062773.1"
                     /translation="MAEVVVITGGTAGVGRAVARRFARQGAKLAILARGKDELRAAAV
                     EVVTLGATAALAIPVDLDDQSEVEAATQRIEAELGPIDVWVDAVTEPKRRSPWWAVAG
                     VVAGAIVGVVGIWLRMRRLR"
     gene            complement(11245..12054)
                     /locus_tag="VFV99_25550"
     CDS             complement(11245..12054)
                     /locus_tag="VFV99_25550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5062774.1"
                     /translation="MNIRSIALTTELALIATRGRIIDRGGYLVVETPDDPGYYHGNLL
                     VLPAAPQIGEVSYWLRKFDDEFGDKPAIRHVTFAWDGISGDEGAGDELRGAGFQIDTH
                     RVMAASSVDPTPAALPSRELKADEVVATADLAWAIGDRHDETYRRFLYRRAEWQRDLV
                     MRGIAKFYGAFDGTTLAASLGLVRVTDVGRYQDVQTLPGYRKRGLARALLAIAARDAL
                     AGGADRVVIVTEPDGAAARLYARAGFHSIELTTSACRYPPDISRPIRVRTD"
     gene            complement(12055..12459)
                     /locus_tag="VFV99_25555"
     CDS             complement(12055..12459)
                     /locus_tag="VFV99_25555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX5062775.1"
                     /translation="MGKEVIRVVAAVIEHEGRYLITQRQANAVLPLLWEFPGGRVEPG
                     EDETKALSREVRGRIGVDVIVGTKLGEHLHEYTNYEVQLTMYSCKLPAEARPYPATVA
                     DLRWVTSREFLDYDFPPADEKTMSKLLGLVRN"
     gene            12549..12941
                     /locus_tag="VFV99_25560"
     CDS             12549..12941
                     /locus_tag="VFV99_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062776.1"
                     /translation="MPIRFVEIKPAESSEALNSEWVILENDGKQPFNTRGCGMTVGRK
                     GSNKKSLLGIIDPGFVLHPGERVRMCTGNPGTQAHGAAPEDGVKNYFLFLPKPYVSGA
                     GVVLTLVLRGLPVSKAEFDPSKPNGVAS"
     gene            complement(12934..>13805)
                     /locus_tag="VFV99_25565"
     CDS             complement(12934..>13805)
                     /locus_tag="VFV99_25565"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5062777.1"
                     /translation="PIAQLGRLVPSGAQPLDVMPVLIEIAALADPQRRDGRPDAIIGR
                     GRALIPVLAAALAPDFAAATVTRALGAGSIAVIAARALGLDDSVAPIFDCALVLLADH
                     ELNASSFTARVAASTDADPYACVAAALATLSGPKHGSASELIARFADEVGGPEHARAA
                     VRALRKKGEVPPGFGHPLYPAGDPRTPPLLVITNELLATTRSAHARRARTLLAIIDAV
                     ADTARREPTKAEPSVDVGLAALIAALGAQASAGSGLFAVARTAGWLAHALEQRAAGYL
                     LRPRARYIGVPVS"
BASE COUNT         2494 a         4459 c         4625 g         2227 t
ORIGIN      
        1 cgcgggagac ctcgcgaaag tggatctcgc aaccggccat gtcatcgccg cacagtgcgg
       61 ctggggcttc gggctgcgcg aatcgcaccc cgagccggcc gactttccga gcacgaccga
      121 gacgctctgc gatcgctgac cagacgtggc agagtacggc catgaattgc cccatctgca
      181 aacggccgct tgttcaggcc ggtcgcacct ggaaatgcga gaccgacgac ggtgcgtggg
      241 tgcgtagcga cgtgctggtg ccgatgctcg agcaggccgc atcgacgctc gtcgaattgc
      301 cgtggcaggc gaatcaagaa gatcacgtac gcaactgccc cgagtgcagc cacgacatgc
      361 agaccgtgaa gctcggcacc gtcgcgctcg accgctgcgt cgagcacggc gtgtggttcg
      421 atcgcaaaga gctcgccgcg gtgctcaagc aagcgaagag cttcaaggcc accgacaagc
      481 acgagcacga gggcatcctc cgcacgctcc agaagctgtt cagctaaaaa aaggagcctg
      541 ggatttctcc caggctccag acgtcacttg gtcactggcg cgtagcgcgt tggctcgagc
      601 gtgaagtgac cgcggccgtg ggtcagccca ccgagcacgc tggcataacc aaacgtctcg
      661 gcgagcggca cctcggcgcg cacgacgcga tcggcaccgc gactatcgag cgcgagcact
      721 tcgccgcgtc gacggccgat gtcgccgatc accgcgccga cctcgtcctc ggggcacgtg
      781 acctcgagga gcatgatcgg ctcgagcatg atcggaccgg cttcggcagc cgccgctttg
      841 aacgcgagcg agcccgcgat ccggaacgca ggctcattcg agtcgcgctc gtggatcgcg
      901 ccgtcgagca gcacgacgcg cacgtcgacg accgggtggc ctcctaagag accatcagcc
      961 atcgcgccac gtacgcccgc ttcgaccgcc ttgatgaacg gcttcggaat cgcgccgccc
     1021 ttgatgcggt cctcgaagac gaggcccgaa ccgggcgccg caggtccgac ttcgatcagg
     1081 acctgtgcaa actgaccagg tccgctgttc tgcttggcgt agcgcagctc gtgacgcgcg
     1141 gtgcgcgtga tcgtcgagcg atacgcgacc tttggctgac ccgttgcgac gacgaggttg
     1201 tgctctttct cgagtcgctc gatggcgata tcgagatgga gctggcccat ccccgcgagc
     1261 aggctttcac cggtttcgcg atcggtctcg atgcgcaacg acggatccgc cgcgatcatg
     1321 cgcgagagca cgagcgagag cttttcgcgc tcggccgcgt tcttcgcttc gatcgagacg
     1381 cgcatcactg gctccggcgc cgtgatcgcc tcgaggacga tcgggcgcgc ggggtcggtg
     1441 agcgtttcgc cgttcgcgag cggagccgcg agaatcgcgc cgatctcgcc agccgcgagc
     1501 tgatcgacct cgacgcgctc gtcggcgacg agctggacga gtcgaccgac gcgcagcttc
     1561 ttgcctcggc cggtcacgac gtgatcgcca cgcgcgagca cgcccgaata cacgcgcacg
     1621 aacgtcatct ggccgtggtc atcaaacccg accttgaagc cgagcgcagc aaggggcgcg
     1681 tcgcgcatgg gcgcgcgcgt ctgcgtaccg tcgatgctct tcaccggttc gcgatcgccg
     1741 ggcgacggca agtacgcgac cacggcgtcg agcagaggct ggacgccgcg gtacttgtac
     1801 gccgaacccg cgagcacggg gaccaggcgg cccgcgatcg tgccgcgccg aagcgccgcg
     1861 cgaatgcgag cagcatcgat cgtgtaccct tcgggtttgc cttcgacgag cgccgcgagg
     1921 atctcgggat cttcgtcggc ggccgcttcg agcaagcgtt cgcgtgcaat taccaagttg
     1981 tcagagagcg tcacggggct ggacgtaaac ttccagccgc gtgtgtcgga ccaccggatc
     2041 gcggtgcgtt cgacgaggtc gaccacgccg acgaggacgt tgtcctcgac gagtggccag
     2101 gtcacagcaa cagcgcgcac gccgaacttc gcgtgcagct cggcaagcga gcgtgcgtag
     2161 tcggcgccga cgcgatcgag cttgttgacg aagacgatgc gcggcacgcg atgtcgattc
     2221 gcttgccgcc acacggtctc ggtctgaggc tctacgccgg cgacgccgtc gagcacgacc
     2281 accgcaccgt cgagcacgcg caacgagcgc tcgacttcga tcgtgaagtc gacgtgaccc
     2341 ggcgtgtcga tcaagtgaat gcgatggccc tgccaatcgg cagtgaccgc cgcagagttg
     2401 atcgtgatgc cgcgccgctg ttcgatttca tcgaagtcgg tatgcgccgt gccatcgtgc
     2461 acctcgccgg ggtcatgaat gacgccggtg tagaggagca cgcgttccgt cagtgtggtc
     2521 ttgccagcat cgacgtgagc gacgatgccg atgttgcgaa ttcgttcgag aggaaacata
     2581 gaagctccgg gattcggata gacgagtccc gtcgcgtcga gcctgaagct cgcacgttgg
     2641 gacgagtggg tgatcggacg cagaaatccc gcgacgggac ctacgcaacg cgcagctgtg
     2701 cctgcgctac tcgcgatagc ccgtgcgggc ttaccgactc accgggcaaa aacacagccg
     2761 ggcgtactcg cgcgagcgcg cagacgcctc ccgagccgaa tcgactcggc gatcgaaacg
     2821 ctgaatgtgc gttgaccgtg gcatagattg cctctaacgc tttcatctaa ggcttccgcg
     2881 gcgtttcgtc aaacgtttcc tcgcaactcc gcagaacgtc gcaccgggtt cagcggagct
     2941 cagcgtgcga ctgaagtcgg aaccgcgtgg gcgcgggcct tgtcgatcac gcgaggcgat
     3001 gtcgcgaggc gcgcctggcc gttcgggctg tacacgacga gcaccgtctc tcgcagctct
     3061 gcccgcgcac gatcccggcc gagctcgcgc agcacccgac gagatgcgat gaacgccgac
     3121 agcggcacgc atgcgatcag cgcgacgacg atgacgagta gtgagttgtg aagcacgaac
     3181 gcgctcacga ccccggcggc cgccgcgacg ctgccgctcg atgcgcggcg aaacactgca
     3241 cgtgcgatgt gatcggcgac gagctgcgaa accggttcgc cttcgcctcg ctgcacgcgc
     3301 caatggcgga gcgagatgac gagcaagatg ccgagcagga tcgcggcgag cagaaccggg
     3361 ccgaacagga tcaagctgac gttgccgagg tcggcgccga catggccttg cggatccttc
     3421 gcggtcggat cggcgacgtc gacgacgaac ggctggccat cgtcgctcgc gctgcgattc
     3481 cccgcgagat cgagtgcgaa cacgcgtacc cgcacgcgac cgggcgtcag ccgaaggccg
     3541 gggcggcaga tgtccgtgtg atccggcgtc gtcgtgacgg tctgggtacc ggaccgatcc
     3601 gtgacggcga tctgcaagag cgcggtgtcc gcggtgacgt ggctcgcgag tcggagccgc
     3661 gccacgggca tgcgctcgag tggctggtac tcgagctcga tctggacttc gtcgaccgtc
     3721 ggcaggtcag gcggagtcgt atcgatcgac gagtcggtct cgaacgagat ggtcgtgccg
     3781 gtgccatgga accagtacaa gaggctcggc tgcaggtcgc gcagcggata cgtgacgacc
     3841 ggatgatcct cgttgcgatc gaacggctgg ctctgcacgg tgatcgtgcg ccggtcggtc
     3901 accgacccga cgtcgtagtc gaagaagcct tccttcgtgc cgagctgcca gatcttcgcg
     3961 ttgagcggaa cgttcttcgc gccatcgacc ggcagcgtgc gcggcgcgag cggctcacaa
     4021 cgatcgacga tgcgatcggc gtgggcgctc gatgcgagcg cgaccaggac accgacgacg
     4081 atcagccgca tgccctcatt atagagcgtc acttcgcgac ggacttcgga attcccgcgc
     4141 gcttcgccca cacgaagtcg atgtcggaag cgcgcacggt taccgtgtca cgcagcgacg
     4201 ttacacacag ccacctgcca tgtcgcactg catcggtctc gggctggtcg agcaggacct
     4261 gggcgcgtcg tatccagtag agcttcgcga ccgcgacgat cgggaatggc gcgaggaaga
     4321 tcggtagctc cgaccgcgcg tggacgaggc cgagcacccc cgcgatggtc atgaccgccc
     4381 acacgatctg ccagcgcaag aggcgccgca cgacctcggc ggcttcgaga tgcgccaccg
     4441 gctcggccgg cgtgcgcttc acggtggcac ggcggacaat cacgatgagg aaccacgcga
     4501 ccacacacaa cggatagagc agcaacagga acaggatctc gaagccgtga tcgtggtgat
     4561 ggtgctcgta gaagccgctg tcgcggcagt ggtcctcgac atcgtcgatc ggcgacggtt
     4621 tgaccgaggt cgcgaacggc gcgctctcgt taccggcgat gtcgatcgcg gtgatccgga
     4681 catctacggt gcccgggccg agatccatgc cgctgcgaca cagatagagc cggttcggcg
     4741 tcgtgtacag ggtcacgaag cggtcagcct ggcggagctc gacgtggatc accgccgtat
     4801 cgggcgcgta gttaccgaac agcgacagcg tctgctggtt cttcaccagg cccgtcgcga
     4861 cgtacgcgtt gacgtcgtgc ggggcctcgg gcggcgcgtc gtcacgcttg tcgttgggcg
     4921 tgaacgagac gccggtcgcc tcgtcggcgt acagcacatt agccgtgagc tcgggccgaa
     4981 gctggtacag atacgcgccg ttctcgagga tgcgcatcac cttggtgttg atcggcaggt
     5041 cctgcgcccc ccattccggg agtgcgattt cattgccgca cgagcaggtg atccgtttcg
     5101 cctccgcgtg acccgcgatc accatcagga ccgcgagcgc gagccgttgc cgcatgctac
     5161 gagtctaaga ggagtcccgg aagtacgtcc atcgaggaac gcacttcgta aacctgtcgg
     5221 ttcgcacgcg tgctcgttct gacgtggtcc gacggacgtc ggtcattcag ggttgagcac
     5281 gataccgtgc cgacgtttcg ccgagccatc gcgacagagt cggatgtgga cgtcgtccac
     5341 gcgatgaacg atccatcgcg ggctgagagg tcgtttgact cggacagggt gtcttgccac
     5401 ccgtgatacg aagaacctag gacgcgccga atctcgatga gcgcgtcggt cgacgcggcg
     5461 tagtcgacgc atgccgacgc cggctcgccg cgctggacgt gcacgacaaa cgacgcgaga
     5521 accgcggcga catcgtcgcg atcgccgacg aggctacaag cggcacagcg atgcacccga
     5581 tcggcgaggc gctttggcac ccgcgcgccg cacgggcagt gctgcgagag cgcggtcgtg
     5641 cgggtggtcg cgcgtgcaac gccaccctca cctccggcaa tggccgccac cgcgcgcgct
     5701 tcgcgatcga gcgcggcgac cagcattcca ggcgtaaacg cggcaaccgc gcttccccac
     5761 gacgacgccc aggcggcgat cgtgcaatct tcgacgacga gctgatagcc gtgcgtggcg
     5821 acgacgccgg ccgcgacctc gcgcgcatgg tcgcgccgcg cgcgggcgcc cgccttggcc
     5881 tctgcgacct gtgcagcgcg ggtccggcga aagctcgccg acagctggtc gtgcctgtag
     5941 ctctgtagcg gcacgccatc gctccgcgcc ttgcgcggtc ccatcggaat cacatcgaca
     6001 ggcgcgaggt tacgcgccgc gcgccggcgg gctcgctttt cctgccgttt ggagaggtga
     6061 tactgcgcac gattggcggc gcgccgcgat cgctcgaggt cgcgctgtcg acgccgctcg
     6121 cgtcgtttcc gtcctcgatc gcgctgcttc tgttcaccgg tacgctcgac gcgggtcagc
     6181 cgcatcgccg tgccggtgtc gtgcgacgct atcgcgaggc acgacacgtt gacgtcgatc
     6241 cccgcggtcc gatcgaccga ctcgatcgcg accttggctc gtcttgccgc cgtcgacggt
     6301 gacacgtacg gcgtcgtcag caccatcaag tgcgcttcgt agcgccaacc gccctcggcg
     6361 ttgggatcgc gatgccgtac caggtcgatc ttgtgccact tgcttgggtc ggtgaggtga
     6421 tagtcaagga tcggctgatt cgacggcgcg gtcggcagcc gcaccggaag cactagggtc
     6481 ccgtcgccaa ggccactgaa cacaatggcg agcggcccgg tgtagctcca ccacgtcttc
     6541 gtggtgagct caacagcgcg cagccgccga ggctgtacaa agtcgccgcc gcgatcggtg
     6601 tacgcgacgc gatgaccggc gagcgtgccg tgcagccgga acgtctccca ctttcgcttc
     6661 ttcgtgtgag acctggctcg gccgggcagc gatttgaagt cgtaccagcg accaatgcgg
     6721 ggactgccgg agcgcttgcc ctgcgcatcg ccaaacacgt gacgctcggc cgcagcccac
     6781 accgtgtcgg cgaggtgcat cgcgagcgcc ttggtcacgg acagtcgcag gtgcggcgca
     6841 ccgtcgagat gcgcggcggc ggcgtcctcg agtgagtcgc gagacaaccc aagtcggtca
     6901 cggacagccg aaggcccgcg ttcgcgctcg tgcctggctt gccagtaggc ccggcagcga
     6961 tcgcgagcgt cgcgctgaac cgcgcgctta agcgagtacg cggcggaaaa catcgcctcg
     7021 atgcgtgcgc gttgccgtgg atcgctcgcg tcgagagcaa gacgcagcac ggacacgccg
     7081 tcgtcgggat ttcgcgtcca cggtggctgc ttgttcggac ctcttgtccg ctttccaaac
     7141 gacccaccga tcgatcctac gcctcacctc tgacgatgac gatggtgtgg agcacgaacg
     7201 caaggctcaa gaaatgatgc gtgctacgca accatcggca gcgcaccacg cactactcga
     7261 cacgctgcgg gttgtcgcgt cgctacacaa ccgtcaaatc gagctacggg agcctcctta
     7321 ggttgttagc ctccgccgac ggtgaatcac gaagaacagt gtcgcgctgc ggagacaacc
     7381 gtcatcatct gctagagtcc gaccccgatg gccgagtcac gcaaacccgc ttacaagcgc
     7441 gtcctcctca agatctcggg agaagcgctg atgggcaacg cgagctacgg gattaacccc
     7501 gaagtgctgc gcgttgtggc ggccgagatt gctgaaaccg cgaagctcgg cgtcgagatc
     7561 gcggtggtgg tcggcggtgg caacatctgg cgcggcgctc aacagggcag tgggatggat
     7621 cgcgcgagcg cggactacgt cggcatgctc gcgaccgtga tgaacgcagt gtcactccaa
     7681 tccgcggtcg agaagagcgg cgtcacgaca cgcgtacaaa ctgcaattcc gatctcgcag
     7741 atcgccgagc cgtacattcg ccggcgcgcg attcgccacc tcgagaaggg ccgcgtcgtg
     7801 atcttcggtg ccggtaccgg caacccgttc ttcacgaccg acaccgccgc cgcgctgcgc
     7861 gcgatcgaga tcggcgccga cgtgctgttg atggcgaaga acatcgacgg cgtctacgac
     7921 aaggatccga agaagcacaa agacgcccgc cgctattcgg ccgtgacgtt caacgacgcg
     7981 ctcgtccatg acctgcgcgt gatggacggg accgccttcg cgctgtgccg cgacaatgat
     8041 ctttcgatcg ccgtgttcga gctcgcgacg cctggcaata tccggcgtgt cgtgtgcggc
     8101 gaaccaatcg gcacgatcgt caagaacaac caagagacgc gcttcgccaa ctagggacta
     8161 gagggacgaa catgatcaac gacatcacga cggatgccga agacggcatg aagaaggcgg
     8221 tcgactcgtt caagcgcgac ctccagaaga tccgcactgg ccgtgcgaac acgtcgatgc
     8281 tcgacggcat caaggtcgac tactacggca cgccgacgcc ggtgaaccag gtcgcgaccg
     8341 tgcaggtcgt cgatgctcgc ctcatcacgg tgaagccgtg ggagaagaac atgatcgcgg
     8401 tgatcgacaa gacgatccgc gcgagcgacc tcggcatcaa cccggtcgcg gacagcgagc
     8461 tcgtgcgcct gccgattccg ccgctgaccc aggagcgccg caaggagctg gcgaaggtgg
     8521 tcggcaagca gaccgaggaa gcgcgcgttg ccgtgcgctc ggcgcgtcgc gatgcgatgg
     8581 acatgatcaa ggacgccgag aaggacaaac agatcactga ggacgagcgc aagaagggtg
     8641 agaccaaggt ccaggagctg acggacaagt acatcggctc catcgaggac atcgcgaagg
     8701 ccaaagagaa agagatcatg gagctgtgac aggcctcgga cctcaggctt caggcctcag
     8761 gtcaagaaat ccccgaggtg gtgatcgttt tcgagttctg gctgaggtct gaggcctgag
     8821 gcctgaggcc tggtatgatt cggtcgcatg tccggaatcc cggcgggggc tgggtcgctt
     8881 attggtaagc gactcggcaa gtacgagatc cttgcgctcc tcgccttggg cggaacggcg
     8941 gagatctacc tcgcgcgcat cggtggtgcg gccgggttcg aaaagtacgt ggtggtgaag
     9001 tgtctgcacg accacctcgc ggacgacacg gacttcgtca agatgttcct cgacgaggcc
     9061 cgcatggccg cgatgctcga ccactcgaac atcgtccaga cgttcgagct cggcgagtac
     9121 acgaaccgct actacatggt gatggagttc ctcgcgggcc tgtcgctcgc gatggtcgtg
     9181 cggcgtgcgc aggaacggct gcaggggggc atgctgccgg tcccgctgat cctcaacgtc
     9241 gccgcgcagg cctgcgccgg tctgcactac gcccacgagc ggagcgcgag cggcaagccg
     9301 ctcaacatgg ttcaccgcga tatctcgccc cagaacctcg tgatcagctt cgagggcgtg
     9361 gtgaaggttg tcgacttcgg catcgcgcgc gccgagcttc gcgagacgaa gacgaaggcc
     9421 ggcacgatca agggcaagtt cgcgtacatg tcacccgagc agtgcgtcgc ggcgaacgtc
     9481 gatcggcgca ccgacgtgtt cgcgctcggc gtgatcgttc acgagctgct cgtcggccgc
     9541 cggctgttca agcgcaactc gccgtacgag acgtatcaag cggtgatcga atgcgccgtc
     9601 gagccgccgt cgcgcatcaa caacacgctc gatcccgcgc tcgatgaggt cgtgatgcgc
     9661 gcggtcgcga aggacaaaga caagcgctat ccgaccgccg aggcgttcgg cgacgcgatg
     9721 ctcggctacc tgcaccatcg cggcaaagcc tcgggcggcg gcgagctcgg ccggttcttc
     9781 gagcaatact tcgcgcaaga gatcgaagag cacggcgagc gcatgcgcga gctgatcgcg
     9841 ggacgtcccg cgtcggtcga gggcgctagc ggctgggacg acgccgatga acaggccaac
     9901 cagaccgtcg acctcagccg caggaaggcg gaggagcagt cgatctcgct cgcaaccggc
     9961 gagatcatgg agctcaacac gggcgacgcg tctctcgatg cgttcgtcgg cgatctcgaa
    10021 gaggagaacg accccgacgc gctgcctgcc gacaagacca acatcgtcca caacccgctc
    10081 gagatgatcg agcagctcaa cgcgcaggtc gcacgcaata ccggcaagac accggtgccg
    10141 cctggctcga tcaccgcgag tggcatcgcg ccactccctg cgccgccgac gggaccgaag
    10201 ggggccgttg gcaaaccggg gtccggcagg ttccccgaca tgccgaacat gccgacgcag
    10261 ctcgcgccgg agacgaagct cgagcacgcg ccgactgcga tcggtgcagc cgtcaatccg
    10321 atgggccaca cgcaagcgga gatgcaggcg cagcccgcgc atttcgatcc caacgtcgcc
    10381 ccggcgggcc cgatgagctc gacgcactac gcagtgcggc cgagccagat gcagggcgcg
    10441 cagcaaccaa acctcggtgc gacggcgcct gcgcccgcgc tgcaaggtca tccgcatggg
    10501 ccgatgcagg ggcatgcgtc ggcgccgatg ccagcgcatg cacccgtgtt gaacgactac
    10561 ccggtgatgc cgggtggcca gcgtccgttc tcgagcgatc tcgcgccgag caatctgtcc
    10621 tcctcaccac agcagcgccc gtcctatggg aatgctccgt tggatccgca agcagcggcg
    10681 aacttgatgt ctcccgtcgg cggcggctat ccgcagaccg actgggaacg tgcggcgggt
    10741 gtgtcggcga aggcgctccc gacgtggctg ctactcgcct tgttcggtgg cgcgctgctc
    10801 ggcgcgctgt tgctcacgct gatgatcgcc aagattttct cgtaggcgca cagcaccgcg
    10861 gcttgcattg catccatgca tggccgaggt cgttgtgatt accggaggca ccgcaggcgt
    10921 cggacgcgca gttgcgcgcc ggttcgcacg acaaggtgca aagctagcaa tcctcgcacg
    10981 cggcaaagac gagctccgag cggctgccgt cgaggtcgtt acgctcggcg cgacagcagc
    11041 gctcgccatt cctgtcgatc tcgacgacca gtccgaagtc gaggccgcga cgcagcgtat
    11101 cgaagcagag ctcggtccga tcgatgtctg ggtcgacgct gtcaccgagc cgaagcgtcg
    11161 ttcgccctgg tgggcggtcg cgggcgtcgt cgccggagcg atcgtcggcg tcgtcgggat
    11221 ctggctgagg atgcgccggt tgcgctaatc ggtacgcacg cgaatcggcc gggagatatc
    11281 cggcgggtac cgacacgccg acgtcgtgag ctcgatcgag tgaaagccgg cgcgcgcgta
    11341 gaggcgagcc gctgcaccgt cgggctcggt gacgatcacg acgcgatccg cgccaccggc
    11401 gagcgcatcg cgcgcggcga tcgcgagcaa cgcacgcgcg aggccacgct tgcgataccc
    11461 cggcagcgtc tggacgtctt gatagcggcc aacgtcggtg acgcgaacga gaccgagact
    11521 cgcggcgagt gtcgtgccgt cgaacgcgcc atagaacttc gcgattccgc gcatgacgag
    11581 gtcgcgttgc cactcggcgc ggcgatacag gaagcggcgg tatgtctcgt catggcgatc
    11641 gccgatcgcc cacgcgaggt ctgccgtcgc aaccacttcg tcggctttga gctcgcggct
    11701 tggcagcgcg gcgggtgtcg ggtcgacgga gcttgccgcc atcacgcggt gggtatcgat
    11761 ctggaagcca gcgccgcgca gctcgtcgcc ggcgccttcg tcgccgctga tgccgtccca
    11821 cgcgaacgtg acgtgacgga tcgccggctt atcgccgaat tcgtcgtcga acttgcgcag
    11881 ccagtacgag acctcgccga tctgcggcgc ggcgggcagc acgagcaagt tgccgtggta
    11941 gtagccagga tcgtcgggtg tctcgacgac gagatagccg ccgcgatcga tgattcggcc
    12001 gcgcgttgcg atcagcgcga gctcggtggt cagcgcgata ctgcgaatgt tcacttaatt
    12061 gcgcacgagc ccgagcaact tcgacatcgt cttctcgtca gcaggcggga agtcatagtc
    12121 cagaaactcg cgtgacgtta cccatcgcag atcagcgacg gtggccggat acggccgcgc
    12181 ttctgccggc agcttgcacg agtacatcgt gagttgaact tcgtagttcg tgtactcgtg
    12241 gaggtgctcg ccgagcttgg tgccgacgat gacatcgacg ccgatgcggc cacgcacttc
    12301 gcgcgacaac gccttcgtct cgtcctcgcc gggttcgacc ctgccgcccg ggaactccca
    12361 gagcagtggt agaacggcgt tggcttgacg ctgggtgatg aggtagcggc cttcgtgctc
    12421 gatcacggcc gcgacaactc ggatcacctc tttgcccatc gcggcctgat tatcgatgga
    12481 tcgctcccga atgcaatcgc gattgaccat ctggcgtcga cgcgaaaggc atgtattctt
    12541 cgcccgaaat gccgattcga ttcgtcgaga tcaagcccgc ggagtcgagc gaagcactca
    12601 acagcgagtg ggtcatcctc gagaacgacg gcaaacagcc cttcaacacg cgtggatgcg
    12661 gcatgacggt cggccgcaaa gggtccaaca agaagtcgct cctcggcatc atcgaccccg
    12721 gcttcgtgct ccatcctggc gagcgcgtgc gtatgtgcac cggaaaccct gggacacaag
    12781 cgcacggtgc cgcccccgag gatggggtga agaactattt tctgttccta ccgaaaccgt
    12841 acgtatctgg cgccggcgtc gtcctgacgc tcgttctacg cgggttgcca gtctcgaagg
    12901 ccgagttcga tccgagcaag ccgaacggcg tcgctagcta acgggaacac cgatgtaacg
    12961 cgcgcgaggc cgaagcaggt aacccgcggc gcgttgctcg agtgcatgcg cgagccagcc
    13021 tgcggtgcgt gcgacggcga acaagcccga tcccgccgac gcctgcgcac cgagcgcggc
    13081 gatgagtgcg gcaagcccga catcgacgct cggctcggcc ttggtcggtt cgcggcgcgc
    13141 agtatctgcg acggcgtcga tgatggcgag cagcgtacgc gcacgtcgtg cgtgcgccga
    13201 acgcgtagtg gcgagtaatt cgttcgtgat cacgagcagc ggtggcgtcc gcggatcgcc
    13261 ggcagggtag agcggatgac cgaaaccggg cggcacttcg cccttcttgc gcagcgcacg
    13321 gactgcggcg cgcgcgtgct cggggccgcc gacctcgtca gcgaagcggg cgatcagctc
    13381 cgacgcggag ccgtgcttcg gtcccgacag cgtggcgagc gctgccgcga cgcatgcgta
    13441 agggtccgcg tccgtcgacg cggcgacgcg tgcagtgaag ctcgacgcat tgagctcgtg
    13501 atcggcgagc agtacgagcg cgcagtcgaa gatcggcgcg acgctgtcat cgaggccgag
    13561 cgcgcgggct gcaatcacgg caatgctgcc ggcaccgagc gcgcgcgtga cggtcgcggc
    13621 ggcaaagtcg ggtgcgagcg cggcggcgag cacggggatc aacgcgcggc cgcggccgat
    13681 gatcgcgtcg gggcgaccat cgcgacgctg cggatcggcg agtgcggcga tctcgatgag
    13741 cacgggcatc acatcgagcg gttgcgcacc actgggcacg aggcgtccga gctgcgcgat
    13801 cggtg
//
LOCUS       DASUFX010000634        11117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_101096,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000634 DASUFX010000000
VERSION     DASUFX010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101096"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2659
                     /locus_tag="VFV99_25570"
     CDS             <1..2659
                     /locus_tag="VFV99_25570"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5062778.1"
                     /translation="CDDGNVVSGDMCSADCKSNETCGNGIIDTAKGEECDDGNTTNAD
                     GCQGNCKLPRCGDGILDANEQCDNGAANANTPNAACRTTCTPRRCGDGVVDNSFSEVC
                     DDNNNTNGDGCSSNCASNETCGNGIIDPAKGEICDDGNARTRDGCSKCQPEVADTLVP
                     GQTPPGRVNPMLVYDPARQRVVMFGGWTPGPNMGDTWEWDGVTWTRMHPKSSPPPRLA
                     PAFAYDVKRRRIVMFGGYQLNYGAPLNDTWVYDGVDWTEILPANKPSPRGASAMAWDA
                     KNGVIVLFGGATSGQCNTCGNSFNTTGDPVNDTWTWNGSNWTQLTPPTTGGYIAPAAR
                     STHTMAYDPVNKLILMRGGQCFGNGVSCTTPDAATYSWDGTGWKNTGASNLGGGLYDG
                     WMAFDTNLGKMVLWRGNSGTTYEWSGSGWTSISLTTPSTRGNMTLTYDVARKQIVLFG
                     GSSQTGTNPTSVQSDTWLRGPGSVAWTPPAPFTQPSARSRAGAAYDPVRRKVVVFGGS
                     CGTSCDLNDTWEWNGRSWANGGGGGPAVRSGSVMDFDTTTRVIRLYGGDTTSTAYTDV
                     YNYNGAWAAQASAGRASVKGATMSYDAQNNRLVTFGGMVNPTTTTNDTWVWTQASGWA
                     LAPAAMKPPGRDQSSTAYDPIRQRTVLFSGNPHTGGTLSDVWEWNNVNWNQITASGGP
                     SQRTGHRLYFNPDSSRVCVFGSASGATTEDLYEWSGTAWFQRTVTNPPATRFAAAAAY
                     DSAAHELVVFSGRDQSTFTNVAATYRVKYVPNTTPEVCTSAQFDYDNDGKYGCADEDC
                     WGVCDALHPPGTTRPANAPFCGDAACNGPEDCLICPGDCGACAGPKCGDFNCDGAENN
                     STCPNDCFCGDKKCDAANGETAATCPSDC"
     gene            2662..7647
                     /locus_tag="VFV99_25575"
     CDS             2662..7647
                     /locus_tag="VFV99_25575"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5062779.1"
                     /translation="MRNHLLHTVFVMLASMVVAACHTVSPDITHCPDVDCPQEKVCDN
                     HGGCAFPEQLQICSGKADGTTCTYRDPTGASVAGQCAGSVCYPLGCGNKFVTPDEVCD
                     DGNNESGDGCAADCRSDETCGNSVVDTAAGEQCDDGNMVDGDGCQSTCKQARCGDGVK
                     DTSLDEECDAGDSNSMNPDAACRPTCVLPRCGDGVLDVMHGEVCDDGGNQAGDGCAGD
                     CKSTEVCGNGVVDSLAGEICDDGNTASGDGCAGDCLSLEVCGNNVLDTARGEICDDGN
                     TVSGDGCSSDCKSLEVCGNMIVDAVTEQCDLGSMNSNAPDAMCRTSCKLPRCGDTIID
                     PMHGEACDNGLMNADAPNACRTNCQNPRCGDNIRDSGEVCDDGNTTSGDGCSANCGSL
                     ETCGNGIIDTAKGEQCDAGAANSNSPNAACRTDCKLRKCGDTVIDNTFGEACDQGINN
                     SDNPNATCRTNCTLQRCGDGVFDNTKGEACDDGNVVSGDGCSNDCRSLEVCGNSIVDT
                     IKGEQCDAGAANANTPNAPCRTTCVLPRCGDSVVDNTLLGEGCDMGAANSNSANAACR
                     TNCQPRRCGDGVLDNLSGEVCDDGNVISGDGCSADCTSLETCGNGVVDTAKGEQCDAG
                     ANNSNSPNAACRTDCKLQRCGDTVLDNLFGEGCDLGPANSNAPNATCRTNCLPQRCGD
                     TITDNTKGEVCDDGNLVSGDNCSADCKSNETCGNGIVDVAKNEQCDAGALNSNAPNAA
                     CRTTCQRQRCGDNVIDNAFGEGCDAGAANSNSPNAACRLNCTPRRCGDGIIDSASGEV
                     CDDANNTNGDGCSSDCKSLETCGNGVVDAIKGEQCDAGAANSNAPNAACRTDCKVQRC
                     GDGIADNTKGEQCDAGAANSNAPNAACRPTCLLPKCGDGVLDNMRGEVCDDNNTVSND
                     GCRSDCASNETCGNGVLDSHVGELCDDGNLRGFDGCSKCTPETAVVLSPAASPPARSL
                     QVLAYDAARQRVVMFGGHSGTTTFGDTWEWDGISWTQLFPAHSPPGRYDAAAAYDPKR
                     HRVVLFGGTTLATGAATQDTWEWDGIDWTQRSPATVPSGRSGAAMAFHPTRGNLVMFG
                     GTNGVTPNNETWEWTGSNWAVVSPSGPPGARAGARMAIDPINKYVVMFGGTADTNTYT
                     LSFNTTTSVWSWATIATGAGPTTGYAAVGMAFDTERAKVTLWGGVNNNTYTWSGATWT
                     LVTGTTPTSPRGEITMTYDIARKQIVLFGGVNGTTPYSDTWLRDTVWAVPAAFVKPTT
                     RFRTQPGYDPLRKRLVVLSGQKENQRFYALADPVQYQDVWEWDGRRWVTGVTTNQPSA
                     RAGSHLEFDVNNRVMRVYGGDYRKINNPGQADTWMSSDMFNFTGTTWTSLSTSGRSDR
                     VQQLGSAWDQSVNKLVTWGGYGLDAMNNFFHYDTTWTWSPTTGWANVSGSPRPPGRRN
                     FTLAYDRVRQRTVLFGGTNDNYLTDTWEFNASTNTWANKTPAISPAPRAGAQLFFNAD
                     SGHVTLFGSSPYNDTEDLWDWNGTAWTEMPLIGTLVPTYSRIVGYDNASHAIVTFGGR
                     DVSAFLATDVFALVKNRPNGSVEACTDSKIDYDNDGNAGCADDECWPQCDPLKSPGTA
                     RPSGAPFCGDGTCNGPEDCLICPNDCTTGCTIKCGDFHCDGTEASTCPSDC"
     gene            7695..10715
                     /locus_tag="VFV99_25580"
     CDS             7695..10715
                     /locus_tag="VFV99_25580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062780.1"
                     /translation="MAGDGSDDELARTATAVGANSPGKDAATPTLGGMLGRYRLEREL
                     GAGGMGVVHAAFDPDLERRVALKVLRSEGGEARQRLLREARALARLTHPNVVTVHEVG
                     SASGRDYVAMELIEGETLAEWLKAAPRTLDEIVNAFIAAARGLAAAHAANLVHRDFKP
                     HNVLRRKDGRIVVTDFGLARGIEAIEFETTMRAKGPQPADDNTPSPLTGLTQTGSVLG
                     TPAYMAPEQWSGDTVGPAADQFAYCVAVWEALAGERPFKGATLEALQADVERGPSQVD
                     DSKLPRRMRAALKRGLDPDPKRRWPSMDALIGALGRADKSRSTLLMIGSFAVVATAAA
                     YAVFATDDKKQVAAPTCEQPVLEPAAIWSPAIANEVRDKTSSDVMRRFDAVFGAWQSA
                     RGVACGLDTPMRSRRLACLDGVIVRIDAVRKASVAGVKPSLDEVSEQLVDPEVCNVDD
                     PPRLPTRLGEAAVTALALAYQPDPKAEWDDKAETEALRKAGDDRCARALIRIAHATND
                     KSTNARDAAEEAVGLADSCGDDRMRADAQVELLAMQVTMFLDPKLQKALATTEAAVRK
                     VSQPDLVAQLDLVKAEIAMESNQWDECIKLADSAIRGFGQNRPLARLEAVQTKARALS
                     GRRQPGDLKTARGELARWRAEAEKVGQPRVLANFDALDVLAQWSMGDVAGANARYAAL
                     YDQIEKSRRKPPEGDPMDGTVVDATGKPVADAVVAAGAAIAADSQTIGYFGNFGDARI
                     AKTDAQGRFTIDHVPKDAIIVAQSGELRSRVKPAKPNDRLVLEPTVTVTGKYDGPNAA
                     QLMVFIVNADRDRSPMYQMLAPIASDGSFTLSGVPVGHLRIGAVQAGTGGRQSFAMQD
                     ITTGPKGLSDVIVKRADSRKVRVIVRSSAVVPLSGAQVFIVAGAISIKTVKEIESVLR
                     SPGMAIEVARPISGETPPDLGKVVAGDLLATFNSAPTGVATACAIGLSGDFSDPGFRE
                     KVQRHIDELEVRCVPLAPDTKAATIEVPPMKRLD"
BASE COUNT         2034 a         3632 c         3825 g         1626 t
ORIGIN      
        1 ctgcgacgac ggcaatgtcg tgtcgggcga catgtgctcg gcggactgca agtcgaacga
       61 gacctgcggc aacggcatca tcgacaccgc gaagggcgag gagtgcgacg atggcaacac
      121 cacgaacgcc gacggctgtc agggcaactg caagctgccg cgctgcggtg acggcatcct
      181 cgacgcgaac gagcagtgtg acaacggcgc ggccaacgcg aacacgccga acgcggcgtg
      241 tcgcacgacc tgcacgccgc gccgctgcgg tgacggcgtc gtcgacaatt cgttcagcga
      301 ggtctgcgac gacaacaaca atacgaacgg cgatggctgc tcgagcaact gcgcatcgaa
      361 cgagacgtgc ggcaacggca tcatcgatcc ggcgaagggc gaaatctgcg acgacggcaa
      421 tgcgcgtacg cgcgatggct gcagcaagtg ccagcccgaa gtcgcggaca cgctcgtgcc
      481 tggccagacg ccgccgggac gggtcaaccc gatgctcgtc tacgatccgg cgcgccagcg
      541 cgtcgtgatg ttcggcggct ggacgccggg cccgaacatg ggcgacacgt gggagtggga
      601 tggcgtcacc tggacgcgca tgcacccgaa gagctcgccg cctccacgtc tcgcgcccgc
      661 gttcgcgtac gacgtgaagc gccgccgcat cgtgatgttc ggcggctacc agctgaacta
      721 cggcgcaccg ctcaacgaca cctgggtcta cgacggcgtc gactggacgg agatcctgcc
      781 cgcgaacaag ccgagcccgc gcggcgcatc ggcgatggcg tgggacgcga agaacggtgt
      841 gatcgtcctg ttcggtggcg cgacgagcgg tcagtgcaac acgtgcggca actcgttcaa
      901 caccacgggc gatccggtga acgacacgtg gacgtggaac ggttcgaact ggacgcagct
      961 gacgccgccg acgacgggtg gatacatcgc gcctgcggcg cgctcgacgc acacgatggc
     1021 gtacgacccg gtcaacaagc tcatcctcat gcggggcggc cagtgtttcg gtaacggcgt
     1081 gtcgtgcacg accccggacg cagcgacgta ctcgtgggat ggcacgggct ggaagaacac
     1141 cggcgcatcc aacctcggcg gcggcctcta cgacgggtgg atggcgttcg acacgaacct
     1201 cggcaagatg gtgctgtggc gtggcaactc gggcacgacc tacgaatggt cgggctcggg
     1261 ctggacctcg atcagcctca cgacaccgag cacgcgcggc aacatgacgc tgacgtacga
     1321 cgtcgcgcgc aagcagatcg tgctgttcgg cggctcgtcg cagaccggca cgaacccgac
     1381 gagcgtgcag agcgacacct ggttgcgcgg gccgggcagc gtcgcgtgga cgccgccggc
     1441 accgtttacc cagccgagcg cgcgcagccg cgcgggcgcc gcgtacgatc cggtgcgccg
     1501 caaggtcgtc gtgtttggtg gctcgtgcgg tacgagctgc gatctcaacg acacgtggga
     1561 atggaacggc cgtagctggg ccaacggtgg tggcggaggt cctgccgtcc gttcgggctc
     1621 ggtcatggac ttcgacacga ccacgcgcgt gattcgcctc tacggcggcg acacgacgag
     1681 taccgcgtac accgacgtct acaactacaa cggcgcgtgg gcggcgcaag catcggcggg
     1741 tcgcgcgtcg gtcaagggcg cgacgatgtc ctacgacgcg cagaacaacc gcctcgtcac
     1801 gttcggcggc atggtgaacc cgaccacgac gacgaacgac acctgggtgt ggacgcaagc
     1861 gagtggctgg gcgctggcgc ctgccgcgat gaagccgccg ggccgcgatc agagctcgac
     1921 ggcctacgat ccgatccgcc agcgcacggt gctgttcagc ggcaacccgc acacgggcgg
     1981 cacgctgtcg gatgtctggg agtggaacaa cgtcaactgg aaccagatca ccgcgagcgg
     2041 cggaccgtcg cagcggaccg gtcatcggct ctacttcaat cccgactcgt cgcgcgtgtg
     2101 cgtgttcggc tcggcgagtg gtgcgacgac cgaagacctc tacgagtgga gtggcacggc
     2161 gtggttccag cgcacggtta cgaatccgcc ggccacccgc tttgcagccg cggcggcata
     2221 cgactcggcg gcgcacgagc tcgtggtgtt ctccggccgc gaccagtcga cgttcacgaa
     2281 cgtcgccgcg acgtatcgcg tgaagtacgt gccgaacacc acgcccgagg tgtgcacgtc
     2341 ggcgcagttc gactacgaca acgacggcaa gtacggctgt gccgacgagg attgctgggg
     2401 cgtgtgcgat gcactgcatc cgcccggtac gacgcggccc gcgaacgcgc cgttctgtgg
     2461 cgacgcggcc tgcaacggtc ccgaggattg cttgatctgc ccgggcgact gcggcgcgtg
     2521 cgccggtccg aagtgcggtg acttcaattg cgacggcgcc gagaacaact cgacgtgccc
     2581 gaacgactgc ttctgcggcg acaagaagtg cgacgccgcc aacggtgaaa ccgcggccac
     2641 ctgcccgagc gattgctaac catgcgcaat catctcctcc ataccgtctt cgtcatgctc
     2701 gcgagcatgg tcgtcgcggc gtgccacacg gtatcgcccg acatcacgca ctgtcccgac
     2761 gtcgactgtc cgcaggagaa ggtttgcgac aaccacggcg gctgcgcgtt ccccgagcag
     2821 ctccagatct gtagcggcaa ggcagacggc accacgtgca cgtatcgcga tccgactggc
     2881 gcgagcgtcg cgggtcagtg cgccggcagt gtctgctatc cgctcgggtg cggaaacaag
     2941 ttcgttaccc ccgacgaggt ctgcgacgac ggcaacaacg agagtggcga cggctgtgcc
     3001 gccgactgcc gctccgacga gacctgcggc aatagcgtgg tcgacaccgc cgccggcgaa
     3061 cagtgcgatg acggcaacat ggtcgatggc gatggctgtc agagcacctg caagcaggcg
     3121 cgctgcggtg acggcgtcaa ggacacgagt ctcgacgagg aatgcgacgc gggcgacagc
     3181 aactcgatga atcccgacgc cgcttgtcgc cccacttgcg tgctcccacg ctgcggcgac
     3241 ggcgtgctcg acgtcatgca cggcgaggtg tgcgacgacg gcggtaacca ggccggtgat
     3301 ggttgtgcgg gtgactgcaa gtcgaccgag gtgtgcggca acggcgttgt cgactcgctc
     3361 gcgggcgaga tctgcgacga cggcaacacc gcgagcggcg atggctgcgc aggcgactgt
     3421 ctgtcgctcg aggtgtgcgg caacaacgtc ctcgataccg cgcgcggcga gatctgcgac
     3481 gacggcaaca cggtgagcgg cgatggctgt tcgtcggact gcaagtcgct cgaggtgtgc
     3541 ggcaacatga ttgtcgatgc ggtcaccgag cagtgcgacc tcggctcgat gaactcgaac
     3601 gcgccggacg cgatgtgccg cacgtcgtgc aagctgccgc gctgcggcga cacgatcatc
     3661 gatccgatgc acggcgaggc ctgcgacaac ggcctcatga acgcggacgc accgaacgcg
     3721 tgccgcacga actgtcagaa cccgcgctgc ggcgacaaca tccgcgacag cggcgaggtc
     3781 tgcgacgatg gcaatacgac gtcgggcgac ggctgctcgg cgaactgcgg ttcgctcgag
     3841 acctgcggca acggcatcat cgacaccgcg aagggcgagc agtgcgacgc tggcgccgcg
     3901 aatagcaaca gcccgaacgc ggcctgccgc acggactgca agctgcgtaa gtgcggtgac
     3961 acggtcatcg acaacacgtt cggcgaggcg tgcgatcagg gcatcaacaa ctcggacaac
     4021 ccgaacgcca cctgccgcac gaactgcacg ctgcagcgct gcggcgacgg agtcttcgac
     4081 aacacgaagg gcgaggcgtg cgatgacggc aacgtggtgt cgggcgacgg ttgctcgaac
     4141 gactgccgct cgctcgaggt gtgcggtaac agcatcgtcg acacgatcaa gggcgagcag
     4201 tgcgacgccg gcgcggcgaa cgcaaatacg cccaatgcgc cgtgccgcac gacgtgcgtg
     4261 ctgccgcgct gcggtgactc ggtcgtcgac aacacactgc tcggcgaagg ctgcgatatg
     4321 ggcgcggcga actcgaacag cgcgaacgcg gcctgccgga cgaattgcca gccgcgccgc
     4381 tgcggcgatg gcgtgctcga caacctgagc ggcgaagtct gcgacgacgg caacgtcatc
     4441 tcgggcgatg gctgctcggc agactgtacg tcgctcgaga cgtgcggcaa cggcgtcgtc
     4501 gataccgcga agggcgagca gtgcgatgcg ggcgcgaaca actcgaacag cccgaatgcg
     4561 gcgtgccgca cggactgcaa gctacagcgc tgcggcgaca cggttctcga taacctcttc
     4621 ggcgagggct gcgatctcgg ccctgcgaac tcgaacgcac cgaacgcgac gtgccgcacg
     4681 aactgcctgc cccagcgctg cggtgacacg atcacggaca acaccaaggg cgaggtctgc
     4741 gacgacggca acctcgtctc cggcgacaac tgctcggccg actgcaagtc caacgagacg
     4801 tgcggcaacg gcatcgtcga cgtcgccaag aacgagcagt gcgatgccgg cgcgctcaac
     4861 tcgaatgcgc cgaacgcggc atgccgcacg acctgccagc gccaacgctg cggcgacaac
     4921 gtcatcgaca acgcgttcgg cgagggctgc gacgccggcg ccgcgaactc gaacagtccg
     4981 aacgctgcat gccggctcaa ctgcacgccg cgccgctgcg gtgacggcat catcgacagc
     5041 gcgtcgggcg aggtctgcga cgacgcgaac aatacgaacg gcgatggctg ctcgtcggac
     5101 tgcaagtcgc tcgagacgtg cggcaacggc gttgtcgatg cgatcaaggg cgagcagtgt
     5161 gacgcgggcg cagcgaactc gaacgcgccg aacgcggcat gtcgcacgga ctgcaaggtt
     5221 cagcgctgcg gcgacggcat cgccgacaac acgaagggtg agcaatgcga tgcaggtgcg
     5281 gcgaactcca atgcgccgaa cgccgcatgc cgtccaacct gcttgctccc gaagtgcggt
     5341 gacggcgtgc tcgacaacat gcgcggcgag gtctgcgacg acaacaacac cgtgagcaac
     5401 gatggctgcc gctcggactg cgcgtcgaac gagacgtgcg gcaacggcgt gctcgattcg
     5461 cacgttggtg agctgtgcga cgacggcaac ctgcgcggct tcgatgggtg cagcaagtgc
     5521 acgcccgaaa ccgccgtcgt gctgtcgccg gctgcgtcgc cgccggcgcg ctcgctacag
     5581 gtgctcgcgt acgatgccgc tcgccagcgc gtcgtgatgt ttggtggcca cagcgggacc
     5641 acgacgttcg gtgatacgtg ggaatgggac ggcatcagct ggacgcagct gttccccgcg
     5701 cactcgccgc ccggtcgcta cgacgcggcc gcggcctacg atccgaagcg tcaccgcgta
     5761 gtgctgttcg gcggtacgac gctcgcgacg ggcgcggcga cgcaggacac gtgggagtgg
     5821 gacggcatcg actggacgca gcgcagtccg gcaacggtgc cgagcggccg cagcggcgcc
     5881 gcgatggcgt tccacccgac ccgcggcaac ctcgtcatgt tcggcggcac gaacggcgtg
     5941 accccgaaca acgagacctg ggagtggacc ggcagcaact gggcggtcgt gtcgccgtcg
     6001 ggaccgcccg gtgcgcgtgc cggtgcgcgc atggcgatcg atccgatcaa caagtacgtc
     6061 gtgatgttcg gcggcaccgc cgacacgaac acctacacgc tgtcgttcaa cacgacgacg
     6121 tcggtctgga gctgggccac gatcgcgacc ggcgcaggcc cgacgaccgg ctacgcggcg
     6181 gtcggcatgg cattcgacac cgagcgcgcc aaggtcacgc tctggggcgg cgtcaacaac
     6241 aacacgtaca cgtggagcgg agcgacttgg acgctcgtca ccggcaccac gccgacatcg
     6301 ccgcgcggcg agatcacgat gacgtacgac atcgcgcgca agcagatcgt gctgttcggc
     6361 ggcgtgaacg gcacgacgcc gtacagcgac acgtggctgc gcgacacggt atgggcagtg
     6421 cctgcagcgt tcgtgaagcc gacaacccgg ttccgcacgc agcctggata tgacccgctg
     6481 cgcaagcggc tcgtcgtgct ctcgggccag aaagagaacc agcgcttcta cgcgctcgcc
     6541 gatccggtgc agtaccagga cgtgtgggag tgggatggcc ggcgctgggt caccggtgtg
     6601 acgacgaacc agccatcggc gcgcgctgga tcgcatttgg agttcgacgt gaacaaccgc
     6661 gtcatgcgcg tgtacggcgg tgactaccgc aagatcaaca acccaggtca ggccgatacc
     6721 tggatgagca gcgacatgtt caacttcacc ggtacgacgt ggaccagtct gtccacgagc
     6781 ggccgctccg atagggtgca acagctcggc tccgcgtggg atcaaagcgt caacaagctc
     6841 gtcacgtggg gtggctacgg cctcgacgcg atgaacaact tcttccatta cgacacgacg
     6901 tggacgtgga gcccgacgac cggctgggcg aacgtcagcg gctcgccgcg tccgcccggg
     6961 cgccgcaact tcaccctcgc gtacgaccgc gtccgtcagc gcaccgtgct gttcggcggc
     7021 acgaacgata actacctgac cgacacctgg gagttcaacg cgtcgacgaa tacgtgggca
     7081 aacaagacgc cggccatcag tccggcgcca cgcgcgggtg cgcagctgtt cttcaatgcg
     7141 gactccggtc acgtgacgct gttcggttcg agcccataca atgacaccga agatctctgg
     7201 gattggaacg gcactgcgtg gaccgaaatg cctctgatcg gcacgctggt accgacctac
     7261 tcgcgcatcg tcggctacga caatgccagc cacgcgatcg tcacgttcgg tggtcgcgat
     7321 gtgtcagcgt tcttggcgac cgatgtgttc gcactcgtga agaaccggcc gaacggtagc
     7381 gtcgaggcct gcaccgattc gaagatcgac tacgacaacg acggtaacgc tggctgcgcc
     7441 gacgacgagt gctggccgca gtgcgacccg ctgaagtcac caggcacggc acggccgtcg
     7501 ggcgcgccgt tctgcggcga cggaacctgc aacggccccg aggactgctt gatctgcccg
     7561 aatgactgca cgacgggctg taccatcaag tgcggcgact tccactgcga tggcaccgag
     7621 gcgagcacct gcccaagtga ctgctaatcg cagcaaccgc gcaggttccc tcgtgatcag
     7681 ctattgtcgc ggcgatggcc ggcgacggta gcgacgacga gctcgcgcga acggcgaccg
     7741 ccgtcggtgc gaattcgccg ggtaaggatg cggcgacccc aacgcttggc ggcatgctcg
     7801 ggcggtatcg gctcgagcgc gagctcgggg ccggcggcat gggcgtggtc cacgcggcgt
     7861 tcgatccgga tctcgagcgg cgcgtcgcgc tcaaggtgct gcgtagcgaa ggcggtgagg
     7921 cgcgccagcg gctgctccgc gaagcgcgtg cgctcgcacg gctgacccat ccgaacgtgg
     7981 tcacggtgca cgaggtcggt tcggcatccg gccgcgatta cgtcgcgatg gagctgatcg
     8041 aaggcgagac gctcgccgag tggctgaagg cggcgccgcg cacgctggac gagatcgtaa
     8101 acgcgttcat cgccgcggcg cgcgggctcg cggccgcaca cgccgccaat ctcgtgcatc
     8161 gcgatttcaa gccgcacaac gtgctgcgcc gcaaggacgg tcgcatcgtc gtcactgact
     8221 tcggtctcgc gcgcggtatc gaggcgatcg agttcgagac cacgatgcgc gcgaagggcc
     8281 cacagcccgc cgacgacaac acaccgagcc cgctcaccgg tctcacccag accggttccg
     8341 tgcttggcac acccgcgtac atggcgccgg agcaatggag tggtgacacc gtcgggccgg
     8401 ctgccgatca gttcgcgtac tgcgtcgcgg tgtgggaagc gctcgccggc gagcgtccgt
     8461 tcaagggtgc gacgctcgag gcgctgcagg ccgacgtcga gcgtggtccg agccaggtcg
     8521 acgattcgaa gttgccacgc cggatgcgtg cggcgctcaa gcgcggcctc gatccagatc
     8581 cgaagcggcg gtggccgagc atggatgcgt tgatcggtgc gctcggtcgc gccgacaaat
     8641 cgcgcagcac gttgctcatg atcggaagct tcgcagttgt cgccaccgcg gccgcgtacg
     8701 cggtgttcgc gaccgacgac aagaagcaag tcgcggcgcc gacgtgcgag cagccggtgc
     8761 tcgagccggc tgcgatctgg tcgccggcga tcgcgaacga ggtccgcgac aagacgtcgt
     8821 cggacgtgat gcggaggttc gatgcggtgt tcggcgcgtg gcaatctgcg cgcggtgtgg
     8881 cctgtggcct cgacacgccg atgcgttcgc gtcggctcgc gtgtctcgac ggcgtgatcg
     8941 tgcgcatcga tgccgtgcgc aaggcgagcg tcgccggcgt gaagccatca ctcgacgagg
     9001 tcagtgaaca gctcgtcgac ccagaagtct gcaacgtcga cgatccaccg cgcttgccga
     9061 cgcgcctcgg tgaggcggca gtgaccgcgc tcgcgctcgc gtatcaaccc gaccccaagg
     9121 ccgagtggga cgacaaggcg gaaaccgagg cgctgcgcaa agcgggcgac gatcgctgcg
     9181 cgcgcgcatt gatccggatc gcgcacgcga cgaatgacaa gtcgacgaac gcgcgggatg
     9241 ccgccgagga agcggtcggg cttgccgact cgtgcggcga cgatcgcatg cgtgccgatg
     9301 cgcaggtcga gttgctcgcg atgcaggtca cgatgttcct cgatccgaag ctgcagaaag
     9361 cgctcgcgac gaccgaggct gccgtccgca aagtctcgca gccggatctc gtcgcccagc
     9421 tcgatctcgt caaggccgag atcgcgatgg agagcaatca gtgggacgag tgcatcaagc
     9481 tcgccgactc ggcgatccgg ggcttcggtc agaaccgccc gctcgctcgt ctcgaagccg
     9541 tacaaaccaa ggcgcgcgcg ctgtccggcc gccgccaacc gggtgatctc aagaccgcgc
     9601 gtggcgagct cgcgcgctgg cgcgccgagg cggagaaagt cggtcagccg cgggtgctcg
     9661 caaacttcga cgccctcgac gtcctcgcgc aatggtcgat gggcgacgtc gccggtgcga
     9721 acgcacgcta cgccgcgctc tacgatcaaa tcgagaagag ccggcgcaag cctcccgagg
     9781 gcgacccgat ggacggcacc gtcgtcgatg ccacgggcaa gccggtggct gatgcggtgg
     9841 tcgcggccgg cgctgcgatc gcagccgact cgcagacgat cggctacttc ggcaacttcg
     9901 gcgacgcgcg catcgcgaag accgacgcgc aaggtcgctt tacaatcgac cacgttccca
     9961 aggacgccat catcgtcgcg cagtccggcg agcttcgctc gcgcgtgaag ccggcgaagc
    10021 caaacgatcg tctcgtcctc gagccgacgg tcacggttac cggcaagtac gacggaccca
    10081 acgcagcgca gctgatggtg ttcatcgtga acgccgaccg cgatcgatcg ccgatgtatc
    10141 aaatgctcgc gccgatcgcg tcagacggct cgttcacgct ctctggtgtt cccgtcggcc
    10201 atctgcggat cggcgccgta caggccggga ccggcggccg ccagtcgttc gcgatgcagg
    10261 acatcacgac cggcccgaag ggcctcagcg acgtcatcgt caaacgcgcc gatagccgca
    10321 aggtgcgcgt catcgtgcgc agctcggcgg tggttccgct ctccggtgcg caggtgttca
    10381 tcgttgccgg cgcgatctcg atcaagacag tgaaagagat cgagtcggtg ctgcggtcgc
    10441 cggggatggc gatcgaggtc gcgcgaccga tcagcggcga aacgccgcct gacctcggca
    10501 aggtcgtcgc tggcgatctg ctcgcgacat tcaacagcgc gccgaccggc gtcgcgaccg
    10561 cgtgcgcgat cggtctatcg ggcgactttt ccgatccggg ctttcgcgag aaagtgcagc
    10621 gtcacatcga cgagctcgaa gtgcggtgtg tgccgctcgc gcccgacacg aaggcggcga
    10681 cgatcgaagt gccgccaatg aagcgtctgg attagttcag cgacgattgg cgccttccgc
    10741 gccggcacgc cgtgttcagg atcgaacgcc aagaacgccg agcgaagccc gtcaaggacg
    10801 ccaccggatg gtcggtcttc aacgcggtct gcacgcgcgg tcgtgcgagc agaacgcgag
    10861 cccgaaacct ttcggatggc gttcttggcg tcttggcgcc ctggcgttcg tctgggagcc
    10921 gcgcagcggc ggacgcacgc tttcgacgcg atgccctagg cagtcctagc gcgtcctagc
    10981 gaaggtcagc gccgtcgacg atcgtgtcgt gctcgggcgc ttcgccgatg cggatcaacg
    11041 cggacgcgag gacgtcgggc gtcgtcgagc ggtagcgggc gcgtagctgt ttgccgccga
    11101 agacgccgac cacggcg
//
LOCUS       DASUFX010000635         6570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_936324,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000635 DASUFX010000000
VERSION     DASUFX010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936324"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(523..3108)
                     /locus_tag="VFV99_25585"
     CDS             complement(523..3108)
                     /locus_tag="VFV99_25585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062781.1"
                     /translation="MPRCTASLVSAPLMIVESTLEAAESDRVEALPAFAPGATIGHFR
                     VVRELGAGGMGIVFEAHDPDLDRRVAIKVVRDRNAGSAAGVRLIAEAQAMARLAHPNV
                     VGVHEVGTIDNQVFLVMELVRGDTLAGWLDRHPRPWRDIVAVFVQAGEGLLAAHHAGL
                     IHRDFKPSNVLIDLSGRARVSDFGLARGDDHEKRKLAQGSEASTVAGTLAYMAPEQRA
                     GEPVDARADQYAFAISLQQALSPKHAVGSPSRRVKNAITRALEIDPDERFAALDQLLA
                     ELKAGIRSRRMTIATIGSGVVMAGLAASLFFTRPAPDDCASGARLVDGVWGPSTRLAQ
                     LAKFLQVRPGAVVTTASTAQLVDDWADRWKLGRKAACAVEGPQRVERLGCLDRGLHEL
                     RAQVTLWQTADATVVDHAVQAAAALPQPQECASHPVAALDDTLRDRIAELAAMLRSGH
                     TRQAHQGVAPMLQLAESQNNPRSLAAALLAAGRIEREAGNLAVARDHFSRAAREAGKA
                     SDDKAMLDALIEESVVIIDLGRPRDSLGLLDAADALQTRSRLDQSERIALVRGDAYGQ
                     AGRAQESIAEITRVLPQIEARAIRDPSARMLLTTALGQLAAAQRQIDPQQGRKTLDRA
                     LALDEQSYGPNHPEVAKTLHDLASVELQLELYKEAGEHLNRAMRIFVASYGERHPMVG
                     ATFMTLAHLETSQGHFEEGKNMYLQAQAALTGVLPEDTPHFSAIEQGLGDIARSQDNC
                     KEAIPHFEKAVKLLEQNGHAENEHALQLTNLGFCLADVGRTDEGRKVLQQSLDELDRL
                     HMPKRWFSEPFAALADLEYAAGNKKKAIELEKKAIAAIADEAGTDAAALRQYEEEQLA
                     AWTKK"
     gene            3209..6040
                     /locus_tag="VFV99_25590"
     CDS             3209..6040
                     /locus_tag="VFV99_25590"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5062782.1"
                     /translation="MDKYFVVEPGAEPHGPYPLPEIANLLAAGTVTPRAILRKAGDAS
                     APPRPTVIDVAPIEAPKPAAAKVMDAAKESAKRSERVERVQRAERQGPPPGVSIPPGT
                     ASFAPPGAAAFAPQGAAAFAPQGAASFAPGAAPVAPPVAPPMAPMAAPTATPPVATPV
                     VGAPNVTPVVVAPSAAPTGAPSVTPVVRAPIAEPSPLQGTDDISVQVDMTNPVEQFDD
                     DGWGGETEIHGDQSSASQPVSDGRTSEHAWPAAGHDASAPTFVPPQASQPHHRAARGT
                     EPQAAWPTDPTQTQPSMSLAVIADRPRRQPEPAAQPALPAPAYELASQAPAAPQLAKP
                     AEDSSPLRFLKQKKLTVGVGIAAVAIGGVAVALIARSGHDTSVKDALVRVSTPSGTGT
                     GFLIDGPDQYIYVATANHLVDRGERVLVERDVNISDKKHYVEAYPETEIVATDPDADL
                     AIIRVKNVAATRFSRLPLAKEPIKDAKIFSYGYPGSSLVKHAGLVSKDGKILSLVRFP
                     AYDERYARVLRENAVDGLLVSTDIEPGFSGGPTTNEAGEVVGINVTKDRAHVGQNGAV
                     SVVALRDLLAKVKPASKQVEPKAEDIVTLLKKVQSEYLLLPLEERSRVRETDFLAASD
                     LPALRQFVNEIRREERNTDNAFIVKLRLSGQAALGMYFARLPGNLLETYRAPSTKTAI
                     TSCALANQRLASFFGELDTNVGKRRNQPLVDTCDELGVRPLAWDLAAATLQWDGKEKD
                     YTVTKIDRMDDEGNVYRASVRISGAANLVELWFGIEQGQPRLKLFDTTENLYAIDSPR
                     TISTSVLQGTWAMKRPRVTDAVDKDAEVESEDKLSVSIGEEHRVSILYTTTQRYFAVS
                     KRSSFPCSGKKSMEVGYTESFVGTLENGVVIATPEKAATPSGADADYCTNRRLPDQII
                     AVKLVGDQLFLYRTDGSAYPETIQLAKQL"
     gene            complement(6065..>6570)
                     /locus_tag="VFV99_25595"
     CDS             complement(6065..>6570)
                     /locus_tag="VFV99_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HEX5062783.1"
                     /translation="IHYLLARTHVTPPSVIVPVSAVMLARRAEYDQVLERFSTAVMPL
                     VDYELEADGSLLVTNETADHYRYFDATPMAEALHRWLDEALHTELRGELDFLVRYQDT
                     KAAMANIIDMPDHLVDLFIKIVTSNGGSLSKRKREEFVMLSDDEVSALEGVVRRHMLG
                     TNAAAPQ"
BASE COUNT         1145 a         2211 c         2112 g         1102 t
ORIGIN      
        1 ggacacgacg cccctacaga aggcgcccac tccgttaccc aggcgcgtgc tacgtaccgg
       61 ggttagtttt ccgtccgcca cgcggacacg acgcccctac ggcaaggcgc ccactccgtt
      121 acccaggcat ctgctacgta ggcgcctgct acgtaccggg gttagttttc cgtccgtcac
      181 gcggacgcga cgcccctaca gaaggcgcca ctccgttacc caggcggcct gctacgtacc
      241 ggggcctgct acgtaccggg gttagttttc cgtccgtcac gcggacgaca cccctacggc
      301 aaggcgccta ctgttaccaa ggcgcctgct acggactcgg tcgcgacggc gccagctact
      361 tcttggtcca ggctgcgagg cggctgctac gtaccggggt tagttttccg tccgtcacgc
      421 ggacgacgcc cctacggcaa ggcgcctact gttaccgagg cgcctgctac gtaccggggt
      481 aatttcgcgt ccgccacgcg gactcgctcg cgacggcgcc agctacttct tggtccaggc
      541 tgccagctgc tcttcctcgt actggcgcag tgccgcggcg tcggtgcccg cttcgtcggc
      601 gatcgcggcg atcgccttct tctcgagctc gatcgccttc ttcttgttgc cggctgcgta
      661 ctcgagatcg gcgagcgcgg cgaacggttc ggagaaccag cgcttcggca tgtgcaaccg
      721 gtcgagctcg tcgagggact gctgcagaac cttgcgaccc tcgtcggtgc gaccgacgtc
      781 cgcaaggcag aacccgagat tcgtgagctg gagcgcgtgc tcgttctcgg cgtggccgtt
      841 ctgctcgagc agctttactg ccttctcgaa gtgaggaatc gcttccttgc agttgtcctg
      901 cgagcgcgcg atgtcgccga gcccttgctc gatcgccgag aagtgcggcg tgtcttcggg
      961 cagcacgccg gtcagcgcgg cctgcgcctg caggtacatg ttcttgcctt cctcgaagtg
     1021 gccttggctc gtctcgaggt gggcaagcgt catgaacgtc gcaccgacca tcgggtgacg
     1081 ctcgccgtac gacgcgacga agatcctcat cgcacgattc aagtgctcgc cggcttcctt
     1141 gtagagctcg agctgcagct cgacggaggc gaggtcatgc agcgtcttgg cgacttcggg
     1201 atggttcggg ccataggact gctcgtcgag cgcgagtgcc cgatcgagcg ttttgcgccc
     1261 ctgctgtgga tcgatctgac gttgtgctgc tgcgagctga ccgagcgcgg tcgtcagcag
     1321 catgcgcgcc gagggatcac gaatcgcacg cgcttcgatc tgtggcagta cgcgcgtgat
     1381 ctccgcgatc gattcctgcg cgcggcccgc ctggccatac gcatcaccgc gcacgagtgc
     1441 aatgcgctcg ctctgatcga gacgcgagcg agtctgcagc gcgtcggcgg cgtcgaggag
     1501 gccgagactg tcgcgcggcc gaccgaggtc gatgatgacg accgattcct cgatcagcgc
     1561 gtcgagcatc gccttgtcgt cgctcgcctt gcctgcctcg cgcgcggctc tcgagaagtg
     1621 gtcgcgcgca actgccagat tgccagcctc gcgctcgatg cgtcctgcgg cgagcaatgc
     1681 ggcggcaagc gagcgcggat tgttctgcga ctcggcgagt tgcagcatcg gtgcgacgcc
     1741 ttgatgcgcc tggcgtgtgt gacccgaacg cagcatcgca gcgagctcgg cgattcggtc
     1801 acgcagcgta tcgtcgagcg ctgcgaccgg atgactcgcg cattcctgtg gttgcggcag
     1861 cgcagcagcg gcttgcaccg cgtgatcgac gaccgtcgca tcagcggtct gccagagggt
     1921 gacctgtgcg cgcagctcgt ggaggccccg atcgagacag ccgagccgct cgacgcgctg
     1981 gggaccctcg accgcgcacg ccgccttccg accgagcttc caacgatcgg cccaatcatc
     2041 gacgagctgt gcggtcgatg ccgtggtcac gaccgcgccc ggccgcacct gcagaaactt
     2101 cgcgagctgc gcgagacgcg tactcggtcc ccacacaccg tcgacgagcc gcgcgccgga
     2161 agcgcagtcg tccggcgcag gacgagtgaa gaataatgac gccgcgagcc ccgccatcac
     2221 gacgccgctg ccgatcgtcg caatcgtcat tcgccgcgag cggatgcctg ccttgagctc
     2281 ggcaagaagc tggtcgagtg ccgcaaaccg ctcgtcggga tcgatttcga gcgcgcgcgt
     2341 gatcgcattc ttcacgcgac gcgacggcga accgacggcg tgcttcggtg agagcgcttg
     2401 ttgcagcgag atcgcgaacg cgtactgatc ggcgcgcgcg tcgaccggct caccggcgcg
     2461 ctgctcgggc gccatgtacg cgagcgtgcc cgcgacggtc gacgcttcgg agccctgcgc
     2521 gagcttgcgc ttctcgtgat cgtcaccacg tgcgagaccg aagtcgctga cgcgcgcgcg
     2581 accgctgaga tcgatcaata cgttgctcgg tttgaagtcg cgatgaatga gtccggcgtg
     2641 atgcgccgcg agtagacctt cgccggcttg cacgaagacc gcaacgatgt cgcgccacgg
     2701 ccgcggatga cgatcgagcc agccggcgag cgtgtcgccg cgcaccagct ccatcacgag
     2761 gaagacttgg ttgtcgatcg tgccgacttc gtgaacgccg acgacgttcg gatgcgcgag
     2821 ccgcgccatc gcctgcgcct cggcgatcag ccgcacgccc gccgccgaac ccgcgttgcg
     2881 atcacgcacg accttgatcg cgacgcggcg atcgagatcc ggatcatggg cctcgaacac
     2941 gatgcccata ccgccggcac cgagctcgcg gacgacgcgg aaatgaccga tcgtcgcgcc
     3001 cggcgcgaat gccggcagcg cttcgacgcg atcgctttcg gcagcctcga gcgtgctctc
     3061 gacgatcatg agcggcgcgg atactagcga tgcggtgcac ctaggcaacg actaaagtgc
     3121 aacaggttca ctgtgtgccg gcgcaccgtt gtttcgcgag gttgcgactg gcacaggcgg
     3181 tgctttgtgc gcgggcgcaa tgagcgccat ggacaagtac ttcgtcgtcg agccgggagc
     3241 cgagccgcat ggcccgtatc cgctgcctga aattgccaac ctgctggccg ccggcaccgt
     3301 cacgccgcgc gcgattttgc gcaaagctgg tgatgcctct gcgccgccgc gcccgacggt
     3361 gatcgacgtc gcgccgatcg aagcgccgaa gcccgcggcc gcaaaggtga tggatgccgc
     3421 gaaggaatcc gcgaagcgct ccgagcgcgt cgagcgagtc cagcgcgccg aacgccaagg
     3481 accgccgccc ggcgtgagca ttccgccggg caccgcatcg ttcgctccgc cgggcgccgc
     3541 agcgttcgcg ccgcagggcg ccgcagcgtt cgcgccgcag ggcgccgcat cgttcgcgcc
     3601 gggcgccgcg ccagtcgcgc caccagtcgc cccgccaatg gcaccgatgg ccgctccaac
     3661 ggcaacgcca ccggtcgcaa cgccggtcgt tggcgcgccg aacgtaaccc cggtcgtcgt
     3721 ggccccgagc gcagcgccga ccggagcgcc gagcgtaacc ccggtcgtca gggcgccgat
     3781 cgctgagcca tcgccgctgc agggcaccga tgacatctcc gtccaggtcg acatgacaaa
     3841 ccccgtcgag cagtttgacg acgacggctg gggcggcgag accgagatcc acggcgatca
     3901 gtcgtcggcc tcgcaaccgg tgtccgacgg ccggacatca gagcacgcat ggccggcagc
     3961 cggacacgac gcgagcgcgc caacattcgt cccgccgcag gcatcgcagc ctcatcatcg
     4021 cgccgcgcgt ggcaccgagc cgcaggcggc gtggccaacc gacccgacac agacgcagcc
     4081 gtcgatgtcg cttgcagtga ttgccgatcg gcctcgccgc cagcccgagc cggctgcgca
     4141 accagcgctc cctgcccccg cgtacgagct ggcgtcgcaa gctccggccg cgccgcagct
     4201 cgcaaagcca gccgaggact cgtcgccgct tcgttttctc aagcagaaga agcttaccgt
     4261 gggcgtcggc atcgcggccg ttgccatcgg cggcgtcgcg gtcgcgctga tcgcgcgtag
     4321 cggtcacgac acgtcggtca aggatgcact cgttcgtgtc agtacgccga gcggaaccgg
     4381 caccggcttt ctgatcgacg gcccggatca atacatctac gtcgcgactg cgaaccacct
     4441 cgtcgatcgt ggcgagcgcg tgctcgtcga gcgcgacgtc aacatcagcg acaagaaaca
     4501 ctacgtcgag gcgtatccgg agaccgagat cgtcgcgacc gacccggacg ccgacctggc
     4561 gatcattcgc gtcaagaacg tggcggcgac ccgcttctcg cgactgccgc tcgcgaaaga
     4621 gccgatcaag gacgcgaaga tcttctcgta cggctatccg ggatcgagcc tcgtgaagca
     4681 cgcgggcctc gtctcgaagg acggcaagat cctcagcctc gttcgcttcc ccgcgtacga
     4741 cgagcgctat gcgcgtgtac ttcgcgagaa cgcggtcgac gggctgctcg tatcgacgga
     4801 catcgaacct ggtttctcgg gcggtccgac gacgaacgag gctggcgagg tcgttggcat
     4861 caacgtgact aaggaccgtg cgcacgtcgg ccagaacggt gccgtgagcg tcgtcgcgct
     4921 gcgcgacctg ctcgccaagg tgaagccggc aagcaagcaa gtcgagccaa aggcggaaga
     4981 catcgtcacg ctgctcaaga aggtgcagag cgaatatctg ttgctgccgc tcgaagagcg
     5041 gagccgcgtg cgcgagaccg acttcctcgc cgcgtctgac cttccggcgc tgcgtcagtt
     5101 cgtgaacgag atccgtcgcg aagagcgcaa caccgataac gcgttcatcg tgaaactccg
     5161 actctccggc caggccgcac tcggtatgta cttcgcgcgg ctgccgggca acctgctcga
     5221 aacgtatcgc gctccctcga cgaagaccgc gatcacgagc tgcgcgctcg caaaccaacg
     5281 cctcgccagc ttcttcggcg agctcgacac gaacgtcggc aagcgccgta accagccgct
     5341 agtcgacacg tgtgacgagc tcggcgtgcg cccgctcgcg tgggatctcg cggccgcgac
     5401 gctgcaatgg gatggcaagg agaaggacta cacggtcacg aagatcgatc gcatggatga
     5461 cgagggcaac gtgtaccgcg cgtcggtgcg catctcgggt gctgcgaacc tggtcgagct
     5521 gtggttcggc atcgagcaag gtcagcctcg cctcaagctg ttcgatacga ccgagaacct
     5581 ctacgcgatc gattcaccgc gcaccatctc gacgagcgtg ctgcagggca cgtgggcgat
     5641 gaagcgccca cgcgtcaccg atgccgtcga caaggacgcg gaggtcgagt ccgaagacaa
     5701 gctctcggta tcgatcggcg aagaacaccg tgtctcgatc ctctacacga cgacgcagcg
     5761 gtacttcgcg gtcagcaagc gcagctcgtt cccgtgcagc ggcaagaagt cgatggaggt
     5821 cggctacact gagagcttcg tcggtacgct cgagaacggc gtcgtgatcg cgacgccgga
     5881 gaaggcagcg acgccgagcg gcgcggatgc ggactactgc acgaaccgcc ggcttcccga
     5941 tcagatcatc gcggtgaagc tcgtcggtga ccagctgttc ctatatcgca ccgacggcag
     6001 cgcgtatccc gagacgatcc agctcgcgaa acagctctaa gacgcgacgc gttcacatcc
     6061 tcgcttattg aggcgctgca gcgttcgttc ccagcatgtg acggcgaacc accccctcta
     6121 gtgccgatac ctcgtcgtcg gacagcatca cgaattcttc gcgcttgcgt tttgacagcg
     6181 acccaccatt cgaggtgaca atcttgatga acaagtcgac gagatgatcc ggcatgtcga
     6241 tgatgttcgc catcgcagct ttggtgtcct gatatctgac taagaagtcc agttcgcctc
     6301 ggagctccgt gtggagcgct tcatcgagcc agcgatgcag cgcctccgcc atcggggtgg
     6361 catcgaaata gcggtagtga tcagctgtct cattcgtcac taaaaggctg ccatccgctt
     6421 cgagctcgta gtcgacgagc ggcatgactg cggtagagaa ccgctcaagc acctgatcat
     6481 actctgcacg tcttgcaagc atgaccgcgg agactgggac gatcacgctc ggcggggtca
     6541 catgcgtgcg cgcgagcaag taatggatca
//
LOCUS       DASUFX010000636        19014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1769411, whole genome shotgun sequence.
ACCESSION   DASUFX010000636 DASUFX010000000
VERSION     DASUFX010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19014
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769411"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFV99_25600"
     CDS             complement(<1..651)
                     /locus_tag="VFV99_25600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062784.1"
                     /translation="MARPIPIRLAFVAILGILLGTAPARAQSKKYPPVTPDKELEDEK
                     RSDLWESTLHPDTRPYAELVRDAKRLIGTNQPADIKVALEKLDTAVKRLPKEADAYQV
                     RGQLFLTQREWAKCADDLGRAEDYGKLDDLTLRTRQRIDLGNCQARAGRYADAENTYV
                     RATASAQAFRGELWMRLGEVRIALGKLDEAIDALTASLDIDNSSALTRWLLMAAYDR"
     gene            complement(630..1670)
                     /locus_tag="VFV99_25605"
     CDS             complement(630..1670)
                     /locus_tag="VFV99_25605"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062785.1"
                     /translation="MKRALLLAFVLAASTAQANVWERAIDADQPERLADDKFESEMRQ
                     GDEHALLASSRNIGKKEVFSHLQNAIAAYRNAAAAKPRDGEPYFRIGKLLYAFYFDCT
                     DQWARTIAFQTCTDQQRDPKSFNVAKAKEIIAAWDAFEARAPLDPRLSVEVSGDTEIL
                     FDRAILHTKLATKADLEAAALDYEKLLSRHDGLEGGTGRVLGNLAETYMMLGRMEESL
                     ETYREALRAYPDTSTLYGYAVALDRDESTRAALDIIKSSGLDQRRAFYEGVQTGGTFF
                     VPEGEVFYYLALVDEAFGYEEDAIGKWQAYIASGAHPEFQPRAKAHLDTLNAHKRRRA
                     IPIESPWPDLFR"
     gene            complement(1667..2683)
                     /locus_tag="VFV99_25610"
     CDS             complement(1667..2683)
                     /locus_tag="VFV99_25610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062786.1"
                     /translation="MRLASTLTVLAALAGVARAEVEAPPIQPQVDPDRGNFWRDMLAP
                     HKDEVDALLLKARQAITVADQALWSDYDPTGLERLRFYREVYGMLRYARKLAPDNLDV
                     LRMLGQCADEHGKTREAIEAFQAAIDVAGMENAGIEATGRLGIIYLRLGKVDDAIRYL
                     RAAQAPVTPGAPLTAQVVVHLSNALAMRGQMTEAIDVLANQIPGSIQYYPNELTLVSF
                     ALAVQYDRDEQRGAAFEVLDRMQNTLQGQLGAMVQNVLAGMRFAPAEDRHYYYGLLYE
                     AAGHYTEARAEWALYAAAGDLPYRRRALEHINAIDALRRAPSADGIGARPLRIRHPRP
                     MVSP"
     gene            2936..3721
                     /locus_tag="VFV99_25615"
     CDS             2936..3721
                     /locus_tag="VFV99_25615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062787.1"
                     /translation="MSDALDAAPDCVPLLLLRARAHIALRRDLDAQTDLREIIRRDPQ
                     CSVAYRLLGELAARRDENESAAIFFREALRLDPDDREAGDWLQIVEASVRPAAVANKL
                     PAPATAAGRFPRAGSAPRFAKGTRPPAEDCVTKPFVGTRRAPSQPPVPQPVRVATGSS
                     PTLQPLVDDAAEFKQTKPGAQPLRRPFSQAPQRNSVPELPGFGDYLVSTGILTRERLR
                     AAQAYQRSMKVQLSTAIVTLGLATPQRIEWAAVAHQSQLARDR"
     gene            complement(3694..4818)
                     /locus_tag="VFV99_25620"
     CDS             complement(3694..4818)
                     /locus_tag="VFV99_25620"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062788.1"
                     /translation="MTTLSTAELLRALTLRDLTDPASGSHAMQLVLAQIHAALSVPVV
                     VHRASPIVSIADNYDRLGYPADGAARDARYTRYVCDGALLRTQTSAMLPPLLASLAAA
                     PPDDVVLSCPGLVYRRDVIDRLHVGEPHQVDLWRIARVALDEVALDAMIRDVVTAALP
                     GTRWRTQPAVHPYTTHGRQIDVEVSGEWIEIGECGLASPALLAASGLASHTGLAMGLG
                     LDRLVMLRKGIDDIRLLRASDPRIASQLYDLEPYRPVSMMPPVRRDLSIVVAAPRDAE
                     ALGDQVRSALGDRADLVEAVEVLATTPYDALPPAAIARLGIAPGQHNVLVRVTLRALD
                     RTLTHDECNVLRDDVYAAIHEGSVHQWARSAAQRSRASCD"
     gene            5447..7567
                     /locus_tag="VFV99_25625"
     CDS             5447..7567
                     /locus_tag="VFV99_25625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX5062789.1"
                     /translation="MGGRSLALVLLAACAAKPAAPMKGAAGGTTTTTTTAGSAATPAP
                     PPIPTAPGPAIPPSPASNATLAEVGLEAASLDRSVDPCVDFYQFACGGWLAANPIPAD
                     RGRVARFTEIDDKNKVAIKALLEEATTKGDPATKKLGDYYASCMDEAAIEKAGTTGIK
                     ALLDKTTKVKDAKSWLAAVTELHKVDISVVWSVTADADLDQSTMNITWLDAAGLGLPD
                     RDYYLKSDFKPQLEAYQQHVGRMLGLAGFAKPEAAAADVVGIETELAKITKSATEKRD
                     VKAADNPTNLKKLAATTKSVDWKAYFKALATTPSAKINLQAPKFFAGLDAVRKQFKPA
                     QWSGYFTYHLLSDSSLALPKAFDDEAFTLTQLLSGATEKQPRFKRCIDATTRGLGELL
                     GKAYVDKHFSAQSKQLAVTLFDALAAAMNDDIATLDWMSDATKKVAQDKLAKVVKMIG
                     FPDKWRSYDFEVKRDDFAGNALRASAFELKRQLAKTGKPVDRSEWLMNTFTVDAYYNP
                     AANNTVLPAGILQPPFFRADRSIAANLGGIGMVIGHELTHGFDDQGAKFDASGNLKNW
                     WQAEDQTKFEDKGKCLAAQYDSFEALPKQYVNGQLTLGENIADLGGVKMAFKAYRALR
                     KDAPKPIVADGFTEDQQFFLGVGQAWCAKMRPPDLQRLLTSNPHAPPKFRVYGALRNL
                     PEFAQAFQCAAGTPMHPAQTCSVW"
     gene            7570..9636
                     /locus_tag="VFV99_25630"
     CDS             7570..9636
                     /locus_tag="VFV99_25630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5062790.1"
                     /translation="MKKLLLAVLLPVLVLASCKQAGKGSYFAKTGAYGPSRPTPYVPP
                     PPKPTVAPEVAWLAECKSPIDQAKKSVDGIIAIPGVRTVDNTLEPFNEVSRNLNNAGN
                     WAHLHSEVNPDAKIREAARTCEQAVQTFVSDLLLDLRIYTAIKSVDISMADANTKRFV
                     ATTLRDYRRAGVELDDAGRNRIKQIDNEVTSLGQQFMKNIADDVRTIDVSDPAKLAGL
                     PQDWIAAHKPDGKGVIKVSTEYPDYIPFITYADDDELRKQLYIAFRSRGDAKNEPILQ
                     QVLTLRAEKALLLGFKDWADYQSDDKMLKGGKAAADFIERVRVLAAARAKKDYAELLA
                     QLRTKVPAAKEVTEWQKAYVENQVKLTKYAVDSTEVRKYFEFEKTLAGLLDITAAIYD
                     IQYVPVAKDPRVWHPDVKVFDVMRKGEKLGRIFLDMHPRPGKYSHAAEFPIVDGIKGK
                     QLPEAALICNFPDPKKANPALMEHGDVVTMFHEFGHLMHHVLGGQVHWARQSGVATES
                     DFVEAPSQMFEEWAWNHDVLSRFAKHYQTGEAIPKDLVDKMRKADHFGKGAWAVQQLL
                     YSALSLRFHQESPSKLNQLDVLKQLQKKYVPFGFVDGTKFHTSFGHLIGYSSMYYTYL
                     WSRVIARDLLTPFDKKGLMATDVTFAYRDKILAAGGTKDAADLVKDFLGRTYNFKAFE
                     KYLSEN"
     gene            complement(9633..10961)
                     /locus_tag="VFV99_25635"
     CDS             complement(9633..10961)
                     /locus_tag="VFV99_25635"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062791.1"
                     /translation="MRRLLLAGAFVLAAMGHAHAGGTRKVKIDSEPSGASVYIGDVDS
                     GVACEPTPCMINAPIGSATIIIRMDKYEPEIAELDVPKGKSPLTQKYKLKSAVGTIKV
                     DTPKGATVRIDDEDKGKAPVEISSSASDPHHVVVSLNGKTVFDDIVEINTGEEFVVKP
                     KMASTNVDDTATVTDDTDGEGGGGGGGSGGGSGGSTGISGSSDGTPRMAYVSVGAAFD
                     VGFRHFTYNNAMTNNLREETEGGQIMGGLAVELWPGRMIGVKPLRGLSLYGRAQFPVA
                     GQTVQGGDLMGTVKTKWSSFEGSLRFRAMFGSLGIEASGGYVQDQMGFDTTVGQDINL
                     MPAVNYQSVRLGAKAIYATDKFEPYLAAETRIVMSGGPVGERFDTAKAGGLRGAIGAT
                     VKLGAISLRGEGSLTSYSWDFTYDNLNDTAQATGATDFVKLISANVNYSY"
     gene            10960..11925
                     /locus_tag="VFV99_25640"
     CDS             10960..11925
                     /locus_tag="VFV99_25640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062792.1"
                     /translation="MAKDDNPRSTVHKEVLRLAVLLAAIALATSRIGLIGHELVGHGG
                     MALACGARIVDVELFWFAGGWIRYQMSEPSLPAILAIAMAGIGLELVVGLLLVQFVRG
                     ATLGRRLVRGIGAGLVVHATWYLATGAFHGFGDGLVLYRALGDARVAVAIAAGLVTCA
                     AAYLAARNVLGTLAATVRGGTRARVLGVIVAAVLAGGLHAGLAWGELRIRRDATYTAT
                     MQHQRDRDIATELAQWQREQAARGTAIDDAQRRAEEVRLAQKHKTFPFVWLLAVATIA
                     SVIAGAVRARKVPDETISNRLLAITVAVALGAICAVILIDVLVAM"
     gene            11935..12825
                     /locus_tag="VFV99_25645"
     CDS             11935..12825
                     /locus_tag="VFV99_25645"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5062793.1"
                     /translation="MQRGVANLLGGSLKPNDPRRFLIEAMVGAMSADGQIDETETAMM
                     EHQIATHPLFQGIGPGAARTLIDLSNDAMQFAGSAIARAPAIAKGLPARIHRLAAFGM
                     AAEVAAADQQIVHGELKFLEALRIAVRIPPHEAENIIRAAKSRQLTPYLDDRYLRIKS
                     LIPIAAEVFALRALARAKANDEQRFKVRDFFAAIPDLVAPTDELDAILYRAFRRPRAP
                     DAQVFGELQWVGQSLPDPVDRYWLVVYTLVAETPSTVQSWRVIPFMGVLQAAFQIVDT
                     DMELAVIDALTFPATLPRPI"
     gene            12958..13458
                     /locus_tag="VFV99_25650"
     CDS             12958..13458
                     /locus_tag="VFV99_25650"
                     /inference="COORDINATES: protein motif:HMM:NF025918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5062794.1"
                     /translation="MTSDTIELVERVRQRLDHQENPVRIAGPVSESAIAAAEEQLECQ
                     FPPSYRAFLRTWGGISLPTHLGIVHDFVGVAPGDGDRDVVARTLRAREERKLGENLVV
                     VGMGAQHQEWFCLDVSRQLPNGEYPVMLFDARDNALDQQFYDDFGQMLEEVMGFVADS
                     LDQPLD"
     gene            13523..14584
                     /locus_tag="VFV99_25655"
     CDS             13523..14584
                     /locus_tag="VFV99_25655"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5062795.1"
                     /translation="MSLVLGLVAFVFTLDDIGPTTFARYFKLIGWWWFAVLAFEIAIT
                     TLDAVAIRAFASPEQAQLPLRSALLAQLAGRSVNAVTPTGSLGEPVKISVLTDNVSNS
                     RAVSTILLYNVVGFTVELMQVAIAAPFMALLLPMPHSLRLAFIAVGIVSGLLAIGIYA
                     LVHRGMLASVARLGVRLRLLSQARFARWEAQLASVDDKLRLVSGARKRDRIAGVTAVI
                     VSRACSLLESLVLLHAIGQSMSVGFIAAYTVGGFAIYMLSSLVPMGLGVAEGGYYSLF
                     AALHYNKAFGVMLVLARRCVLLAYAAIGLVLVATTETVQRAKEQLRVRRESMQTIPLP
                     VRPASVSVAPPIAVPREAD"
     gene            complement(14581..15276)
                     /locus_tag="VFV99_25660"
     CDS             complement(14581..15276)
                     /locus_tag="VFV99_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062796.1"
                     /translation="MLGRIVLLVAIAIAVIYVLRARRQLAVLAQQIRDLRAHALKPVT
                     YRPVGSPELADELSAATAQAEKAGFTMLGDYLEESEVREGMPMRWFVDARGTTFGWMS
                     PFEVRGKREIVLVLMSHELASQTITARAPKASALMRPPFVHAQTVAMDTSFKDTVAKH
                     REHANLDDETRGFVPVKTFEQLSHELERMRGKVITWRESQPDDELLDADLRTLLGAQY
                     KRLASAMRRRLAS"
     gene            15463..16020
                     /locus_tag="VFV99_25665"
     CDS             15463..16020
                     /locus_tag="VFV99_25665"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5062797.1"
                     /translation="MRSEPYRPLKRVEYERLAALGFFDNEKVELLLGMVVPMTPIDPA
                     HSESTASLADALRTRLGDRARVREQHPLAASDISEPEPDVAVVPTQQYWNEHPTRAFL
                     IVEVANTSLRKDKGPTALVYGLADVDEYWIVNQVEAVVEVYRDRHNGEWRQRTTHRRG
                     DTIALVAFPDVQIAVSEILPPVEKP"
     gene            complement(16024..17604)
                     /gene="pckA"
                     /locus_tag="VFV99_25670"
     CDS             complement(16024..17604)
                     /gene="pckA"
                     /locus_tag="VFV99_25670"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX5062798.1"
                     /translation="MFSLDKQGITVSEIHRNATPAFLYEAALKFEKGTAISSTGAMIA
                     YSGKKTGRSPTDKRVVDEPEVRDDIWWGTVNIKLDPQSFLINRERGIDYLNTRDRLYV
                     VDGYAGWDPKYQLKIRVISSRAYHALFMHNMLIRPTDAELATFGEPDYVIYNAGGFPA
                     NRHTAGMTSTTSIDLSFSRREFVILGSEYAGEMKKGVFTIMNYLMPKQGVLSMHCSAT
                     QARDGSDTSILFGLSGTGKTTLSADPKRLLIGDDEHCWTDAGVFNIEGGCYAKVVKLS
                     KEQEPDIWNALQFGAVLENVVFDPYHRVVDYNDTSITENTRGSYILDTIPNAKLPAVA
                     GHPKNVIFLTADAFGVLPPVSKLTPGQAMYHYISGYTAKVAGTEVGVKDPEPTFSACF
                     GGPFLVWHPTKYAELLAEKLRKHGAQTWLVNTGWTGGPYGTGSRIKLKYTRAIIDAIH
                     SGALHNAPTVEDPIFGLQVPTSCPEVPPEMLQPRNTWTDKAAYDEKAKRVASLFKENF
                     KKYEAQASAEVRGGGPKV"
     gene            complement(17629..>19014)
                     /locus_tag="VFV99_25675"
     CDS             complement(17629..>19014)
                     /locus_tag="VFV99_25675"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5062799.1"
                     /translation="VLVQVGAAGVSAVDTMFGEMTLTAGTDSLVLDDFLYPLPPIPVG
                     GTVTQVVGILALRQMASKLEPRSGADVMQGPPTLSTFGPALSYVRVGTTGVPTFPAGS
                     ELEVVLSGPAQGNTDVTIASSSGSLVVAGGKVTVLSGMTTATVLVDGVSPNTDVTLTA
                     TLGSTVMTAHVRVLGAAETPTAVTLSPMTAAVAPNGAVTLTATLDIPAAAGGTSVALA
                     VNPQAAGTLNPATAVVVAADTLSATFTYTDASGANATVTATLGGSSSSTMITVSTGAS
                     HLVLNEIDYDQVNTDNAEYVEIYNPTPAAISLNNVALVLINGANNQAYPTATSTIDLS
                     PAGSIPALGYLVIAGANIAVQPPALKLDPGWTTDQVQNGSPDGIALVNTATNTLIDAL
                     SYEGSITMAEVPGIANPVSLVEGTVLSNAIVDTNTVVGALCRSPNGKDTDNANADWKL
                     CTTLTPGAANP"
BASE COUNT         3159 a         6174 c         6329 g         3352 t
ORIGIN      
        1 gcgatcgtag gcggccatca gcagccagcg cgtgagcgcg ctcgagttgt cgatgtcgag
       61 cgacgcggtc agcgcatcga tcgcctcgtc gagcttgccg agcgcgatcc ggacctcccc
      121 gagccgcatc cacagctcac cgcgaaacgc ctgggcactc gcggtcgcac gcacgtacgt
      181 gttctcggcg tcggcgtagc gaccggcgcg ggcctggcag ttgccgaggt cgatgcgttg
      241 ccgcgtgcgc agcgtgaggt catcgagctt gccgtagtcc tcggcacgac cgagatcgtc
      301 ggcgcacttc gcccactcgc gctgggtaag gaacagctgg ccgcggacct gatacgcatc
      361 ggcttccttc ggcaggcgct tcaccgcggt gtcgagcttc tcgagcgcga ccttgatgtc
      421 ggcgggttgg ttcgtaccga tgagacgctt ggcgtcgcgg acgagctctg catacggccg
      481 cgtgtcggga tgcagtgtcg actcccagag atcggagcgc ttctcgtctt cgagctcttt
      541 gtccggggtg accggcgggt acttcttcga ttgtgctcgg gccggtgcgg ttccgagcag
      601 gatgccgagt atggcgacga acgcgagcct tatcggaata ggtctggcca cggtgactcg
      661 atcggaatcg cacgccggcg cttgtgagcg ttcagcgtgt cgagatgtgc tttcgcgcgg
      721 ggctggaatt cgggatgcgc accggatgcg atgtacgcct gccactttcc gatcgcgtcc
      781 tcttcgtagc cgaacgcttc gtcgacgagc gccaggtagt agaagacctc gccctccggc
      841 acgaagaacg tgccgccggt ctggacgcct tcgtagaacg ctcggcgctg gtcgagaccg
      901 gagctcttga tgatgtcgag tgcggcgcgc gtgctctcgt cgcgatcgag cgcgaccgcg
      961 tagccgtaga gggtcgacgt gtcggggtac gcgcgcagcg cttcgcgata cgtctcgagc
     1021 gactcctcca tgcggccgag catcatgtac gtctcggcga ggttgccgag cacgcgaccg
     1081 gtgccgccct cgaggccgtc gtggcgggac agcagcttct cgtagtcgag cgccgctgct
     1141 tcgagatcgg ccttcgtcgc cagcttcgtg tggaggatcg cgcggtcgaa caggatctcg
     1201 gtgtcaccgc tcacctcgac ggagagtcgc ggatcgagcg gcgcacgcgc ctcgaatgca
     1261 tcccacgcgg cgatgatttc tttcgccttt gcgacattga agctcttcgg gtcgcgctgt
     1321 tgatcagtgc aggtctggaa cgcgatggtg cgcgcccact gatccgtgca gtcgaagtag
     1381 aacgcgtaga gcagcttgcc gatgcggaag tacggctcgc cgtcgcgtgg cttcgcggcg
     1441 gcagcgttgc gatacgccgc aatcgcgttt tgcagatgcg agaagacttc cttcttgccg
     1501 atgtttctcg agctcgcgag cagcgcgtgc tcgtcgcctt gccgcatctc ggattcgaac
     1561 ttgtcgtcgg cgagtcgctc gggctggtcg gcgtcgatcg cgcgttccca cacgttcgct
     1621 tgcgccgtcg acgctgcaag cacgaatgcg agcagtaggg cgcgcttcac ggcgacacca
     1681 tcgggcgagg gtgacggatg cggagcggtc gcgcgccgat accatcagcg gatggcgcgc
     1741 ggcgcagcgc atcgatcgca ttgatatgct cgagtgcgcg gcgccgatag ggcaagtcac
     1801 cggcagcggc gtagagcgcc cattcggcgc gcgcctcggt gtagtggcct gcggcttcgt
     1861 agagcaagcc gtagtagtag tggcgatctt cggccggcgc aaatcgcatt cccgcgagca
     1921 cgttctgcac catcgcgccg agctgaccct gcagcgtgtt ctgcatgcgg tcgagaacct
     1981 cgaacgcggc accgcgctgc tcgtcgcggt cgtattgcac cgcaagcgcg aagctcacga
     2041 gcgtgagctc gttcgggtag tactggatcg agcctggaat ctggttcgcg agcacgtcga
     2101 tcgcttcggt catctggccg cgcatcgcga gcgcgttcga caggtgcacg acgacctgcg
     2161 cggtgagcgg cgcacctggc gtgaccggcg cttgcgcggc gcgcaggtaa cggatcgcgt
     2221 cgtcgacctt gccgagccgc aggtagatga tgccgagccg gccggtcgct tcgatgccgg
     2281 cgttctccat gcccgcgacg tcgatcgccg cctggaacgc ttcgatcgct tcgcgcgtct
     2341 tgccgtgctc gtctgcgcac tgaccgagca tgcgcagcac gtcgaggttg tcgggcgcga
     2401 gcttgcgcgc gtagcgcagc attccgtaaa cctcgcggta gaaccgcagg cgctcgaggc
     2461 cggtcgggtc gtaatcgctc cacaacgcct ggtccgcgac cgtgatcgcc tgccgtgctt
     2521 tgagcagcag cgcgtcgacc tcatctttgt gtggggcgag catgtcgcgc cagaagttgc
     2581 cgcgatccgg atcgacctgc ggttggatcg gcggcgcctc gacttcggcg cgcgcaacac
     2641 ccgcaagggc ggccagcacc gtcagtgtgg aggcgagcct catcacgttt gaaagtatgc
     2701 cggtgacctc gcggggttgc caggcagacg cagcgtccga aggccggtcg cgcctcctcg
     2761 atctggggtc cacggcctcc ggcggggatc acacggattc gctgctattc caggggcttg
     2821 gggtgggaaa cctcgtggcc caccccttgc tgtatccgac tacgtggacc gcggagtttt
     2881 ggcgacggca accgagctgt tcgaagctcg tcattttcca ggcgttgtcg ccctggtgag
     2941 tgacgcgtta gacgcggcgc cggattgtgt cccgttgctg ctcctgcgcg cgcgggcgca
     3001 tatcgcgctt cgccgtgatc tcgacgcgca gaccgacttg cgcgagatca tccggcgcga
     3061 tccgcagtgc agtgtcgcgt atcggctcct tggcgagctc gctgcacgcc gcgacgagaa
     3121 cgagtccgct gcgatctttt ttcgcgaggc gctgcgcctc gaccctgacg atcgtgaagc
     3181 cggcgactgg ctccagatcg tcgaagcatc agttcgacca gccgcggtag caaacaaact
     3241 cccggcaccc gctaccgcgg ctggtcgttt tcctcgtgca ggcagcgcgc ctcgctttgc
     3301 gaagggcacg cgtccacccg ccgaggattg cgtcaccaag ccgttcgtcg gcacgcgtcg
     3361 cgcgccatcg cagccgccgg ttcctcaacc ggtgcgcgtc gcgaccggtt cgtcgcccac
     3421 gctgcagccg ctcgtcgatg acgctgcgga attcaagcag acgaagccgg gtgcccagcc
     3481 gctgcgccgg ccgttctcgc aagcgccaca gcgcaactcg gtgcccgagc tgcccggatt
     3541 cggcgactac ctggtctcga ccggcatcct cacgcgcgag cgcctacgcg ccgcacaggc
     3601 gtaccagcgc tcgatgaagg tgcagctgtc gaccgcgatc gtcacgctcg gcctcgcgac
     3661 gccacagcgg atcgagtggg ccgccgtcgc gcatcagtcg cagctcgctc gcgatcgctg
     3721 agccgcactg cgagcccatt ggtgcacgct tccctcgtgg atcgcggcgt agacgtcgtc
     3781 gcgcagcacg ttgcactcgt cgtgcgtcag cgtgcgatcg agagcgcgca gcgtcacgcg
     3841 tacgagcacg ttgtgctgac cgggcgcgat gccgagccgc gcgatggctg cgggcggtag
     3901 cgcgtcgtac ggcgtcgtcg cgagaacctc gacggcctcg acgaggtcag cgcgatcgcc
     3961 aagcgcgctc cgcacctggt caccgagtgc ttcggcgtcg cgcggtgctg ccaccacgat
     4021 cgacagatcg cggcgcaccg gcggcatcat cgacacgggc cgatacggtt cgaggtcgta
     4081 gagctggctc gcgatgcgcg gatcgctcgc gcgcaggagc cggatgtcgt cgatgccttt
     4141 gcgcagcatc acgaggcgat cgaggccgag ccccatcgcg agcccggtgt gtgacgcgag
     4201 acccgacgcg gcgagcagcg cgggtgacgc gagaccgcac tcgccgatct cgatccactc
     4261 gccgctcacc tcgacgtcga tctgccggcc gtgtgtcgtg tacggatgca ccgcgggctg
     4321 cgtgcgccag cgcgtgccgg gcaacgccgc cgtgacgacg tcgcggatca tcgcgtcgag
     4381 cgcaacttcg tcgagcgcga cgcgcgcgat gcgccagagg tcgacttggt gcggctcgcc
     4441 gacatgcagg cgatcgatga catcgcgccg gtagacgaga ccgggacacg acagcaccac
     4501 gtcgtcgggc ggtgctgcgg cgagcgaggc caacagcggc ggcagcattg cggatgtctg
     4561 ggtgcgcaag agcgcgccgt cgcagacgta gcgcgtgtag cgtgcgtcgc gcgcagcacc
     4621 gtcggctgga tagccgaggc ggtcgtagtt gtcggcgatc gacacgatcg gcgacgcgcg
     4681 atgcacgacg acaggaacgg agagggcagc gtggatctgc gcgagcacga gctgcatcgc
     4741 gtgagagcca gacgcgggat cagtcaaatc gcggagcgtg agcgcacgca gcagctccgc
     4801 tgtggaaagc gtggtcatga caaacctcgg ggcagagggc gggaggagtg gacgaggccc
     4861 cgtgcccagg tcagtcagcg gacgaacgac acccgctgcc cgaagggcac ggggttcgta
     4921 aatcgacgag tccgcatacc gaactctaac gcatcgcgac tgcgacgccg actgaattct
     4981 cgggcacgcg tgttgctgcg ctggcttatg agcctcggga aggagcttcg agatgccgcg
     5041 ccaacaccta cgagctgtgt cgcgtgacga tccgggagcg ctcattcgac aacgcattga
     5101 tgacgtcgtc gcggcgattc gcgcgaagga tatcgacggc gtcatggcaa tctacgcgcg
     5161 caacatcgtg tcgttcgatc tcggtccgcc gctgagctac ggcggggccg acaataaggg
     5221 tcgagcgtgg cgcgcagtgt tcgcagccta tacgggtccc atccgttgta cgtggtcatc
     5281 gacaatcgtt tgccggtcgc gaccggcctg acctggaacc ggccagcgac gaccgccgag
     5341 atcgagggac cgcgggcgac cgagcagatg gctcgtcgct aacggacaca ccgccgcgcc
     5401 ggccgtgcgc tgacagcgat tccccgtgtg gtgatacctt caccacatgg gtggacgcag
     5461 cctcgcgctg gttttgctcg ccgcgtgtgc ggcgaagcca gccgctccga tgaaaggcgc
     5521 cgcaggcggt acgacgacca cgacgacgac ggccggtagc gcagccacgc ccgcaccgcc
     5581 gccgataccg acggcacctg ggccggcgat tccgccgtca ccggcgagca acgcgacgct
     5641 cgccgaggtc gggctcgaag cggcatcgct cgatcgctcc gtcgatccgt gcgtcgactt
     5701 ctatcagttc gcatgcggcg gctggctcgc agcgaatccg atcccagccg accgtggccg
     5761 cgtggcccgg ttcaccgaga tcgacgacaa gaacaaggtc gcgatcaagg cgctgctcga
     5821 agaagcgacg accaagggcg atcccgcgac gaagaagctc ggcgattact acgcgtcgtg
     5881 catggacgaa gcggcaatcg agaaggccgg gaccaccggc atcaaggcgt tgctcgacaa
     5941 gacgacgaag gtcaaagacg cgaagagctg gctcgcagca gtgaccgagc tgcacaaggt
     6001 cgacatctcg gtggtgtgga gcgtgacagc cgacgctgat ctcgaccagt cgacgatgaa
     6061 catcacgtgg ctcgatgccg cggggctcgg gctgcccgac cgcgactact acttgaagtc
     6121 ggacttcaag ccgcaactcg aagcgtatca gcagcacgtg ggacggatgc tcgggctcgc
     6181 cggcttcgcg aagccggaag cggccgccgc cgacgtcgtg gggatcgaga ccgagctcgc
     6241 gaagatcacg aagtccgcga ccgaaaagcg cgacgtcaag gccgcggaca acccgacgaa
     6301 cctcaagaag ctcgcggcga cgacgaagtc ggtcgactgg aaggcgtact tcaaggcgct
     6361 cgcgactacg ccgagcgcga agatcaactt gcaggcgccg aagttcttcg ctggcctcga
     6421 cgcagttcgc aagcagttca agccggcgca gtggtcaggc tacttcacgt atcacctgct
     6481 cagcgactct tcgctggcgc tgccgaaggc attcgacgac gaagcgttca cgctgaccca
     6541 gctgctcagc ggcgcgaccg agaagcagcc tcgcttcaag cggtgcatcg acgcgacgac
     6601 gcgcgggctc ggtgagctgc tgggtaaggc gtatgtcgac aagcacttct cggcgcagtc
     6661 gaagcagcta gcggtgacgc tcttcgacgc gctcgccgcc gcgatgaacg acgacatcgc
     6721 gacgctcgac tggatgagtg atgcgacgaa gaaggtcgcg caggacaagc tcgccaaggt
     6781 cgtcaagatg atcggcttcc ccgacaagtg gcggagctac gacttcgagg tcaagcgcga
     6841 cgacttcgcg ggcaacgcac tgcgcgcgtc ggcgttcgag ctcaagcgcc agctcgcaaa
     6901 gacgggcaag cctgtcgatc gcagcgagtg gctcatgaac acgttcaccg tcgacgccta
     6961 ctacaacccg gccgcgaaca acacggtgct gccggcgggc atcctgcagc cgccgttctt
     7021 ccgcgccgat cgttcaatcg cggcgaatct cggtggcatc ggcatggtga tcggccacga
     7081 gctgacgcac ggcttcgacg atcagggtgc gaagttcgac gcctccggca atctcaagaa
     7141 ctggtggcag gccgaggacc agacgaagtt cgaggacaag ggcaagtgcc tcgcggcgca
     7201 gtacgactcc ttcgaggcgc tgccgaagca gtacgtcaac ggccagctca cgctcggcga
     7261 gaacatcgcc gacctcggcg gcgtgaagat ggcattcaag gcgtatcgcg cgctgcgcaa
     7321 ggacgcgccg aagccgatcg tcgccgacgg attcaccgag gatcagcagt tcttcctcgg
     7381 cgtcggtcag gcgtggtgcg ccaagatgcg gccgccggac ctacagcgcc tgctcacgag
     7441 caacccgcac gcgccgccaa agtttcgcgt ctacggcgca ctccgcaact tgccggagtt
     7501 cgcgcaagcg ttccagtgcg ccgcggggac gcctatgcat cccgcgcaga cctgttcggt
     7561 gtggtgaaca tgaagaagct cctcctggcc gtcctccttc ccgttctcgt cctcgcctcg
     7621 tgcaaacaag ccggtaaagg ctcctacttc gcaaagaccg gcgcttacgg accgagccgg
     7681 ccgacgccat acgtgccgcc gccgccgaag ccgaccgtcg cgccggaagt cgcgtggctt
     7741 gcggagtgca aatcgccgat cgatcaggcg aagaagagcg tcgacgggat catcgcgatc
     7801 ccgggcgtgc gcaccgtcga caacacgctc gagccgttca acgaggtgtc gcgcaatctc
     7861 aacaacgccg gcaactgggc acacctgcat tccgaggtga atcccgacgc gaagattcgc
     7921 gaagctgcgc gcacctgcga gcaggcggtg cagacgttcg tctccgacct gctgctcgac
     7981 ctccgcatct acaccgcgat caagagcgtc gacatctcga tggcagacgc gaacacgaag
     8041 cggttcgtcg cgacgacgct gcgcgactac cgccgcgcag gtgtcgagct cgatgacgcc
     8101 ggtcgcaacc gcatcaagca gatcgacaac gaggtcacgt cgctcggcca gcagttcatg
     8161 aagaacatcg ccgacgacgt gcgcacgatc gacgtgagcg atccggcgaa gctcgccggt
     8221 ctgccgcagg actggatcgc cgcgcacaag cccgacggca agggcgtgat caaggtctcg
     8281 accgagtacc ccgattacat cccgttcatc acgtacgccg acgacgacga gctcaggaag
     8341 cagctctaca tcgcgttccg cagccgcggc gatgcgaaga acgagccgat cctgcagcag
     8401 gtgctcacgc tgcgcgccga gaaggcgctg ctgctcggct tcaaggactg ggccgactac
     8461 cagagcgacg acaagatgct caagggcggc aaggcagccg ccgacttcat cgagcgcgtg
     8521 cgcgtgctcg ccgccgcgcg cgcgaagaag gactacgccg aattgctcgc gcagttgcgc
     8581 acgaaggtgc cggccgcgaa ggaagtcacc gagtggcaga aggcgtacgt cgagaaccag
     8641 gtcaagctga cgaagtacgc cgtcgactcg acggaggtcc ggaagtactt cgagttcgag
     8701 aagacgctcg cgggcctgct cgacatcacc gcggcgatct acgacatcca gtacgtcccc
     8761 gtcgcgaagg acccgcgcgt ctggcacccg gacgtcaaag tgttcgacgt catgcgcaag
     8821 ggcgagaagc tcggccgcat cttcctcgac atgcatccgc gcccgggcaa gtactcgcac
     8881 gcggccgagt tcccgatcgt cgacggcatc aagggcaagc agctgcccga ggcggcgctg
     8941 atctgcaact tccccgatcc gaagaaggcg aaccccgcgc tgatggagca cggcgacgtc
     9001 gtcacgatgt tccacgagtt cggccacctc atgcatcacg tgctcggcgg tcaggtgcac
     9061 tgggcgcggc agagcggggt cgcgaccgag agcgactttg tcgaggcccc gtcgcaaatg
     9121 ttcgaggagt gggcgtggaa ccacgacgtg ctgtcgcggt tcgcgaagca ctaccagacc
     9181 ggtgaggcga ttccgaagga cctcgtcgac aagatgcgca aggcggatca cttcggcaag
     9241 ggcgcgtggg cggtgcagca gctgctctat tcagcgctgt cgctgcgctt ccatcaggag
     9301 agcccgagca agctgaacca gctcgacgtg ctcaagcagc tgcagaagaa gtacgtgccg
     9361 ttcggctttg tcgacggcac gaagttccat acgtcgttcg gccacctgat cggctactcg
     9421 tcgatgtact acacgtatct gtggtcgcgc gtgatcgcgc gcgatctgct gacgccgttc
     9481 gacaagaagg gtctgatggc gaccgacgtc acgttcgcgt accgggacaa gatcctcgct
     9541 gccggcggca cgaaggacgc agccgacctc gtcaaagact tcctcggccg cacgtacaac
     9601 ttcaaggcgt tcgagaagta cctcagcgag aactagtagc tgtagttgac gttcgcggag
     9661 atcagcttca cgaagtccgt cgcgcccgtc gcctgcgcgg tgtcgttcaa gttgtcgtag
     9721 gtgaagtccc acgagtaact cgtcagcgag ccttcgccgc ggaggctgat cgcgccgagc
     9781 ttcacggtgg caccgatcgc gccgcggagc ccgcccgctt ttgcggtatc gaagcgttcg
     9841 ccgaccgggc cgcccgacat cacgatgcga gtctcggcgg cgagatacgg ctcgaatttg
     9901 tcggtagcgt aaatcgcctt cgcgccgagg cgcaccgact gatagttcac ggcgggcatg
     9961 aggttgatgt cctgcccgac ggtcgtgtcg aagcccatct gatcctgcac gtagccgccg
    10021 ctcgcttcga tgccgagact gccgaacatc gcgcggaagc gcaggctgcc ctcgaagctc
    10081 gaccactttg tcttcaccgt gcccatcaga tcgccgccct gcacggtctg cccggcgacg
    10141 ggaaactgcg cgcggccgta cagcgacaat ccgcgcagag gcttcacgcc gatcatgcgg
    10201 ccgggccaca gctcgacggc gagcccgccc atgatctgac cgccctcggt ctcctcgcgc
    10261 aggttgttcg tcatcgcgtt gttgtaggtg aagtgacgga acccgacgtc gaacgccgcg
    10321 cccacgctca cgtacgccat gcgcggtgtg ccgtcgctgc tgccagagat gcccgtcgag
    10381 ccgccgctgc caccgcccga gccaccgccg ccgccaccgc cttcgccgtc ggtatcgtcg
    10441 gtgaccgtgg cggtgtcgtc gacgttcgtc gaggccatct tcggcttcac gacgaattcc
    10501 tcgccggtgt tgatctcgac gatgtcgtcg aacacagtct tgccgttgag cgagacgacg
    10561 acgtgatgcg gatcggatgc cgacgacgag atttcgacgg gcgccttgcc cttgtcttca
    10621 tcgtcgatgc ggacagtcgc gcccttgggg gtatcgacct tgatggtgcc gaccgcgctc
    10681 ttcagcttgt acttctgcgt gagcggcgac ttgcccttcg ggacgtcgag ctcggcgatc
    10741 tccggctcgt atttgtccat gcggatgatg atcgtcgcgc tgccgatcgg tgcgttgatc
    10801 atgcacggcg tcggttcgca cgcgacgccg gagtcgacgt cgccgatgta gacggatgca
    10861 ccggacggct cgctgtcgat cttcaccttg cgcgttccgc ccgcgtgggc atgtcccatc
    10921 gcggccagca cgaatgcgcc ggctagcagc agccgcctca tggctaaaga tgataacccg
    10981 aggtcgacgg tgcacaagga agtcttacga ctcgcggtgt tactcgcagc tatcgcgctt
    11041 gcgacgagcc gcatcggcct catcggccac gagctcgttg gccatggcgg aatggccctc
    11101 gcgtgtgggg cgcgtatcgt cgatgtcgag ctgttctggt tcgcgggtgg ctggattcgc
    11161 tatcaaatga gcgagccgag cctgccggcc atcctggcga tcgccatggc cgggatcggg
    11221 ctcgagctgg tggtcggcct tctgctcgtg cagttcgtgc gcggcgccac gctcggccgg
    11281 aggctcgtgc gcgggatcgg ggcggggctc gtggtccatg cgacctggta cctcgcgacc
    11341 ggcgcgttcc acggcttcgg ggacgggctc gtgctgtatc gcgcgctcgg cgatgctcgg
    11401 gtagccgtcg cgattgctgc cgggctcgtg acctgcgccg cggcctacct ggccgcacgc
    11461 aacgtgctcg gcacgctcgc cgcgacggtt cgcggtggca cccgtgcgcg ggtgctcggt
    11521 gtgatcgtcg ccgcagtgct cgcgggtggt ctgcatgccg gtctcgcgtg gggcgagctc
    11581 cggatccggc gcgacgcgac gtacacggca acgatgcagc accagcgcga tcgcgatatc
    11641 gcgacagagc tcgcgcagtg gcaacgcgag caagccgcac gcggcaccgc gatcgatgat
    11701 gcgcagcgcc gcgccgagga agtgcggctc gcccagaagc acaagacgtt tccgttcgta
    11761 tggctgctcg cggtcgcgac gatcgcctcg gtgattgccg gagccgtgcg cgcgcgcaag
    11821 gtgcccgacg agacgatcag caatcgtttg ctcgcgatca cggtcgccgt ggctctcggt
    11881 gcgatctgcg ccgtgatcct gatcgacgtg ctcgtggcca tgtagactgc aggggtgcaa
    11941 cgcggtgttg cgaatcttct cggcggcagt ctcaagccga acgatccgcg gcgcttcctc
    12001 atcgaggcga tggtcggcgc gatgagcgcg gacggtcaga tcgacgagac cgagacggcg
    12061 atgatggagc accagatcgc gacgcatccg ttgttccagg gcatcggccc aggagcggca
    12121 cggacgctga tcgatctctc gaacgatgcg atgcagtttg ccggcagcgc gatcgcgcgt
    12181 gcgcctgcca tcgcgaaagg cctgcccgca cgcattcacc ggctcgccgc gttcggcatg
    12241 gccgccgagg tcgccgccgc cgatcagcag atcgtgcacg gcgagctgaa gttcctcgag
    12301 gcgctgcgaa tcgccgtccg catcccgccg cacgaggccg agaacatcat tcgggcggcg
    12361 aagagccggc agctcacgcc gtatctcgat gatcgctacc tgcgcatcaa gtcgctcatc
    12421 ccgatcgcgg ccgaggtctt cgcgttgcgc gcgctcgccc gtgcgaaggc aaatgacgag
    12481 cagcggttca aggtccgcga cttcttcgcc gcgattcccg atctcgtcgc gcccaccgac
    12541 gagctcgacg cgatcctcta tcgtgcgttc cgtcggccac gcgcgcccga cgcgcaggta
    12601 ttcggcgagc tccagtgggt cggacaatcg ctgcccgatc cagtcgatcg ctactggctc
    12661 gtcgtataca cgctcgtcgc cgaaactccg tcgacggtgc agagctggcg cgtgatccca
    12721 ttcatgggcg ttctccaggc agcgtttcag atcgtcgaca cggatatgga gctcgcggtg
    12781 atcgacgcct tgacttttcc cgccacattg cctcggccga tctgatcgcg atccgcgccg
    12841 agttgatgag cacgcgagtg aatgtcaacc acgacgtgcg gccgagtttt tcgcgcgcca
    12901 gtctcgtgaa ttgagccggt tgttttttcg acgacccgtg cataatcgag cttcgtcatg
    12961 acgtcggaca ccatcgagtt agttgaacgc gttcgtcagc gactggatca ccaggagaat
    13021 cccgtgcgca tcgcgggccc tgtttccgag tccgcgatcg cggccgcaga agaacaactc
    13081 gagtgtcagt tcccgccgtc gtatcgcgcg ttcctacgca cgtggggcgg catctcgctt
    13141 cccacgcatc tcggtatcgt tcacgacttc gtcggcgtgg caccgggcga cggtgatcgc
    13201 gacgtagtgg cccgcacact ccgtgcacgc gaggaacgta agcttggcga gaacctcgtc
    13261 gtggttggta tgggcgccca acatcaagag tggttttgcc tggacgtttc gcgccaactc
    13321 ccgaatggcg aatacccggt gatgctgttc gacgcccgcg ataacgcgct cgaccagcag
    13381 ttctacgacg acttcggcca gatgctcgaa gaggtgatgg gcttcgtcgc cgacagccta
    13441 gatcagcctt tggattgagc ggccggactg ctatagctcc cggctaatga gcagaccgac
    13501 ctgggctaga tgggtcacca gggtgtccct ggtcctcggc ctcgtcgcgt ttgtcttcac
    13561 gctcgacgac atcggaccga cgacgttcgc gcgctacttc aagctcatcg gctggtggtg
    13621 gttcgcggtc ctcgcgttcg aaatcgcgat cacgacgctc gatgcggtcg cgatccgcgc
    13681 attcgcttcg ccggagcaag cccagctgcc gctgcgctcg gcgctgctcg cccagctcgc
    13741 gggtcgctcg gtcaacgcgg tcacgccgac gggcagcctc ggcgagccgg tcaagatctc
    13801 ggtcctcacc gacaacgtct ccaactcgcg cgcggtctcg acgattctgc tctacaacgt
    13861 cgtcggcttc accgtcgagc tgatgcaggt cgcgatcgcg gcgccgttca tggcgctcct
    13921 tctgccgatg ccgcacagcc tgcgcctcgc gttcatcgcg gtcggcatcg tctccggcct
    13981 cctcgctatc ggcatctacg cgctcgttca tcgcggcatg ctcgcctcgg tcgcgcgcct
    14041 cggcgtcagg ctccgccttc tctcgcaagc ccggttcgcg cgctgggaag cccagctcgc
    14101 ttccgtcgac gacaagctcc gcctcgtctc gggcgcccgc aagcgcgatc gcatcgccgg
    14161 cgtcaccgcg gtcatcgtgt cgcgtgcgtg ctcgctgctc gaatcgctcg tcctgctcca
    14221 cgcgatcggt caatcgatgt cggtcggctt catcgccgcc tacaccgtcg gtggcttcgc
    14281 gatctacatg ctgtcgtcgc tcgtcccgat gggcctcggt gtcgccgagg gtggctacta
    14341 ctcgctgttc gccgcgctcc actacaacaa ggcgtttggc gtgatgctcg tgctcgcgcg
    14401 tcgctgcgtg ctcctcgcct acgcggcgat cggcctcgtg ctcgtcgcga cgaccgagac
    14461 cgtgcaacgt gcgaaagagc agctccgcgt ccggcgcgaa tccatgcaga ccattccgct
    14521 accggtccgc ccggcgtcgg tctccgtcgc cccgccgatc gctgtcccgc gcgaagccga
    14581 ttagctcgcg agtcgccggc gcatcgccga cgcgagtcgc ttgtactgcg cgccgagcag
    14641 cgtgcgcaga tccgcgtcga gcagctcgtc gtcgggctgg ctctcgcgcc acgtgatcac
    14701 cttgccgcgc atccgctcga gctcgtggct cagctgctcg aacgtcttta ccggcacgaa
    14761 gccgcgcgtc tcgtcgtcga gattcgcatg ctcgcgatgc ttcgcgaccg tgtccttgaa
    14821 cgaggtatcc atcgcaacgg tctgcgcgtg cacgaacggc ggccgcatca gcgccgatgc
    14881 cttcggcgcc cgcgccgtga tcgtctggct cgccagctcg tgcgacatca gcacgagcac
    14941 gatctcgcgc ttgccgcgga cctcgaacgg cgacatccag ccgaacgtgg tgccgcgcgc
    15001 gtcgacgaac cagcgcatcg gcatgccctc gcggacttcg ctctcctcga ggtaatcgcc
    15061 gagcatggtg aaaccggcct tctcggcctg cgctgtcgcc gcggacagct cgtcggcaag
    15121 ctcggggctg ccgaccggcc gatacgtgac cggcttgagc gcgtgtgcgc gcaggtcgcg
    15181 aatctgctgc gcgagcacgg cgagctgccg gcgcgcgcgc agtacgtaga tcacggctat
    15241 cgcgatcgcg acgagcaaca cgattctgcc gagcacgacc agaccttagt cgagagcgga
    15301 cggcagtcaa tggccagcgg agttgccgtc gtccaacaca cggacggcgc tgcgttcgat
    15361 ctcgtcgaat tcacacgcgg agcgctgcgg aactgtcgtt gctgtggacc gcggcatgaa
    15421 aggagtacag tcatggaagc gatgtcgaac ccagagaccc tcatgcggag cgaaccgtat
    15481 cgtccgctca agcgcgtcga atacgaacga ctcgccgcgc tcggcttctt cgacaacgag
    15541 aaggtcgagt tgctgctcgg catggtggtt ccaatgacgc cgatcgatcc ggcgcattca
    15601 gagtcgaccg ccagcctagc cgacgcgttg cggactcggc ttggtgaccg ggctcgagtc
    15661 cgcgagcagc atcccctggc ggcttcggat atctcggagc cggagccaga cgtcgccgtg
    15721 gtgcccaccc agcagtactg gaacgagcac ccgacgcgcg cgtttctgat cgtcgaggtc
    15781 gccaatacat ccttgcgcaa ggacaagggc cccacggcgc ttgtctatgg gctcgccgat
    15841 gtcgacgaat attggatcgt caatcaggtc gaggccgtcg tcgaggtgta ccgcgaccgc
    15901 cacaacggcg aatggcgcca gcgcacgacg catcggcgcg gcgacaccat cgcgctggtc
    15961 gcgtttcccg acgtgcagat tgcggtcagc gagatcctgc cgccggtcga gaagccgtag
    16021 cggctagacc ttcgggccgc cgccgcgaac ttcagccgag gcctgcgcct cgtacttctt
    16081 gaagttctcc ttgaacagcg acgcgacgcg ctttgctttc tcgtcgtacg cggccttgtc
    16141 ggtccacgtg ttgcgcggct ggagcatctc gggcggcact tcggggcagc tcgtcgggac
    16201 ttggaggccg aagatcggat cctcgacggt cggcgcgttg tgcagcgcgc ccgagtgaat
    16261 cgcgtcgatg atcgcgcgcg tgtacttgag cttgatgcgc gagcccgtgc cgtacggacc
    16321 gccggtccag cccgtgttca cgagccaggt ctgcgcgcca tgcttgcgga gcttctcggc
    16381 gagcagctcg gcgtacttcg tcgggtgcca gacgaggaac ggaccgccga agcaggcaga
    16441 gaacgtcggc tcgggatctt tgacgccaac ttcggtgccg gcgaccttcg cggtgtagcc
    16501 cgagatgtag tgatacatcg cttggccggg cgtgagcttc gagacaggcg gcagcacgcc
    16561 gaacgcgtcg gcggtcagga agatgacgtt cttcgggtgg ccggcgacgg cggggagctt
    16621 cgcgttgggg atcgtgtcga ggatgtagct gccgcgcgtg ttctcggtga tcgaggtgtc
    16681 gttgtagtcg acgacgcggt ggtacgggtc gaacacgacg ttctcgagga cggcgccgaa
    16741 ctggagtgcg ttccagatgt cgggctcttg ctccttggag agcttcacga ccttcgcgta
    16801 acagccgcct tcgatgttga agacgccggc gtcggtccag cagtgctcgt cgtcgccgat
    16861 gagcaggcgc ttcggatcgg ccgagagcgt ggtcttgccg gtgcccgaga ggccaaacag
    16921 gatcgacgtg tcgctaccgt cgcgcgcctg ggtggcggag cagtgcatcg acagcacgcc
    16981 ctgcttcggc atcaggtagt tcatgatcgt gaacacgccc ttcttcatct cgccggcgta
    17041 ttccgagccg aggatgacga actcgcggcg gctgaacgag aggtcgatcg aagtcgtcga
    17101 cgtcatgccc gccgtatgcc tgttcgcggg aaacccaccg gcgttgtaga tgacgtagtc
    17161 gggctcgccg aacgtcgcca gctcggcgtc ggtcggccgg atcagcatgt tgtgcatgaa
    17221 cagcgcgtga taggcgcgcg acgagatcac gcggatcttg agctggtact tcgggtccca
    17281 accggcgtag ccgtcgacga cgtagaggcg gtcgcgcgtg ttgaggtagt cgatgccgcg
    17341 ctcgcggttg atgaggaacg actgcgggtc gagcttgatg ttgacagtgc cccaccagat
    17401 gtcgtcgcga acttcgggct cgtcgaccac gcgcttgtcg gtcgggctgc ggcccgtctt
    17461 cttaccggag tacgcgatca tcgcgccggt ggaggagatt gcagttccct tctcgaactt
    17521 cagtgccgct tcgtagagga aggccggcgt tgcattgcgg tggatctcag agaccgtgat
    17581 gccttgcttg tcgagggaga acatgatgcg ttgctttata caacaccttc agggattggc
    17641 ggcccccggc gtcaacgtcg tgcatagctt ccagtcggcg ttggcgttgt ccgtatcctt
    17701 accgttgggt gagcggcaca gcgcgccgac cacggtgttg gtatcgacaa tggcattgga
    17761 cagcacggtc ccctccacca acgagacggg gtttgcgatc ccgggcactt ccgccatcgt
    17821 gatcgatccc tcataggaca atgcgtcgat cagcgtgttg gtcgcggtgt taacgagggc
    17881 gattccgtcg ggtgaaccgt tctgcacctg gtcggtggtc caacctggat cgagctttag
    17941 cgccggtggc tgaactgcaa tgttggcgcc tgcgatcacc agataaccca gcgccggaat
    18001 cgatcctgcg ggtgagagat cgatggtcga ggtggcggtc gggtacgcct ggttgttggc
    18061 gccgttgatg aggaccagtg caacgttgtt gagactaatt gccgcagggg tcggattgta
    18121 gatctccacg tactcggcgt tgtcggtgtt gacctggtcg tagtcgatct cgttgaggac
    18181 gaggtggctc gcgccggtcg acaccgtgat catggtgctg ctcgacgatc cgccgagcgt
    18241 cgcggtgacc gtcgcgttgg cgccgctcgc gtcggtgtac gtgaacgtcg cgctcagcgt
    18301 gtcggccgcg acgacgacgg cggtcgccgg gttcagcgtg cccgcggcct gtggattgac
    18361 ggcgagcgcg acggacgtgc cacctgctgc ggcgggaatg tcgagcgtcg cggtgagcgt
    18421 gaccgcgccg ttcggtgcga ccgctgcagt catcggcgac agggtgacgg cggttggcgt
    18481 ttcggcggcg ccaagcacgc gtacgtgcgc ggtcatcacg gtgctgccga gcgtggcggt
    18541 gagcgtgacg tcggtgttgg gcgagacgcc gtcgacgagc accgtcgcgg tcgtcatgcc
    18601 gcttagcacc gtgaccttac cgccggcgac gacgagcgag ccggagctgc tcgcgatcgt
    18661 gacgtcggtg ttgccttgtg cgggacccga cagcacgacc tcgagctcgc tgccggccgg
    18721 gaacgtcggg acgcccgtgg tgcctacgcg cacgtagctg agcgcggggc caaacgtcga
    18781 cagcgtcggc ggaccctgca tcacgtcggc gccgctgcgc ggctcgagct tcgacgccat
    18841 ctgacggagc gcgaggatgc cgacgacttg cgtgaccgtc ccaccgacag gaatcggcgg
    18901 cagcggatag aggaagtcgt cgagcacgag gctatcggtt ccggccgtca gcgtcatctc
    18961 gccgaacatc gtgtcgacgg ccgagacgcc cgcagcgcca acctggacga gcac
//
LOCUS       DASUFX010000637         6369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_519691,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000637 DASUFX010000000
VERSION     DASUFX010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519691"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFV99_25680"
     CDS             <1..449
                     /locus_tag="VFV99_25680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062800.1"
                     /translation="ITASMRVVLAFLFAACADPTPRKVQLIDAPPAGEVEPIISGELA
                     QAIAANDSLLVYVGASWCEPCRHFHEAAEAGQLDDKLGGLRLLVFDADRDTDALESAG
                     YHSNLIPLFAVPRIDGRASGHQIEGAIKNRDAVTQLVPRIKTLLAR"
     gene            complement(446..1366)
                     /locus_tag="VFV99_25685"
     CDS             complement(446..1366)
                     /locus_tag="VFV99_25685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5062801.1"
                     /translation="MRRLGLVALLAACNGSSMSQSGDDTQPMIDAPKAPMIDAAPVCT
                     PLGTCDWLDSYQRHIVGVLAGVEDISPGVKIAHRESVGERNATRQFLDEAFAALNIPS
                     QHQNYTSGSNVGANVIGTLDGPGSLIIIGAHFDGVAAGPAAADNATGVAIVLSLARYL
                     RDVPRTHKIAFALFDQEELGLIGSREYVKTLTAGDIASVHIYDMVSFDGDGDHAVELW
                     SPAPAVEAMYQAKGAVAGMPIQPVTFELSDHQAFVEAALPATGVSEEFVGGDHTPNYH
                     KSTDTYANVQFDHLARVTHLAFAVLDEAVR"
     gene            1487..2548
                     /locus_tag="VFV99_25690"
     CDS             1487..2548
                     /locus_tag="VFV99_25690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062802.1"
                     /translation="MTTRYTVLERLGGGGQAEVFRGVSESIQGFKKPVAIKRVLPHLT
                     SNPQFVAMFLDEARLSLFLQHANVVQVFDISKAADGTYFLVMEFVDGCDLKGLIHHQR
                     TVDIGLTLYMMIEAAKGLHYAHTLEHPQTGKPLGIVHRDVSPPNIMLSKNGEVKVVDF
                     GLAKAESQVEITDEGVVKGKFAYLAPEAAFGKEVDARADVFALGIITWEMLTGRRLFY
                     NDDPHKTIELVRAARVPSITALNPKVDPELDAIVRKALARHPENRTASAADFADELAQ
                     HLFGNEMKVTSRNVAAAVQAIRAAKKPSSAAKALIDALIADEVAKMTSIVDTQAAEDA
                     PKPAEIVDTADWASELIDK"
     gene            2618..3199
                     /locus_tag="VFV99_25695"
     CDS             2618..3199
                     /locus_tag="VFV99_25695"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX5062803.1"
                     /translation="MATSSSIEGRGRHESRYWGWPMVIGILLIIGGLFALYASVLTSF
                     ISVIYLGVMLMVVGVLEVISGFRMRHTQPFLVYLLAGLLAIVVGFMFLDQPKVGVLAM
                     SLLICGYLFASGLFRGVVAIAERYPRWGWDLAYGIVAILLGIYLLKEWPIASFWLLGT
                     LVAIEIIARGVSLVAASWVIRDIQHHRLAHGFA"
     gene            complement(3196..4269)
                     /locus_tag="VFV99_25700"
     CDS             complement(3196..4269)
                     /locus_tag="VFV99_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062804.1"
                     /translation="MRNVLFALCAVASCKGASRSSEPATTTPPATEAGTPTVATASAD
                     AATSTATPVAIPGGEHGIGFDDLRFARGIGRVVAPAGGTGTIALIDPATRALTTIAGF
                     GATTGTTGHEDGSTSADESAGHLYAIDRTKLEVVLIDEKTAKIVGSAKLASSPDYVRW
                     VEATHELWVTQPDEYRIEVFTLTGTTPKHAAFIAVTGGPESLVIDGKRAFTHLWKGTT
                     VVLDVTTRKQTASWPNGCTGSRGIALDGAHNILFVGCGEGRAVALDASSGKQLDVIVV
                     GAGVDIIDFNPKLSHLYVPSAKTATFSIIGVAAGGKFSLLGTAEAALGAHCVTADDRN
                     HAWVCDPKAGRLLEIEDTYPASL"
     gene            complement(4289..>6369)
                     /locus_tag="VFV99_25705"
     CDS             complement(4289..>6369)
                     /locus_tag="VFV99_25705"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5062805.1"
                     /translation="DIEPVYDQATLVDESLASVRDAILIGILLSLIVIAISLRDWRAG
                     LIAALPVPITLLGTFAVMRAFGMTLNLMSLGGLAISIGLVVDDAIVVTEGIVRRLEEG
                     HTPAEAAELGTSDMLGPVIGTTLTTVVVFAPLALLSGTTGSFLGALALTLAIAVLLSM
                     LMALTLIPVLAHRLHARKRVERDDKPDRISRTIAWLLRHRLVAVLVIVFLIGAGFGAT
                     RITPTGFLPHMDEGAFVIDFALPPGTSLEESARITAAIDDVLRETKEVSSFARRNGTE
                     LGPATATLQNTGDIMVRLVPPGDRPPIDDVIDHVRDKLHDRVPESRFEYIQVLQDVLA
                     DLAGNPAPIEVRILGNDPRQLESYAEQAGERLAKLDELEDFFDGREGYAPILRGQLEL
                     GQIARLNLDAATIGTDLTIALEGRVVGEVLRPERAIGVRLRYPDAIRNDPTALARSSI
                     AYGPQSLALGEVITFDRPLAPAVLKRDGLRAAVVMTAAAKDGDLGGAEQAVVAALHDV
                     QPPPGVQIAIGGQAETARAARRELYVIALTATLLVLFVLVLQLHSLRFALVVLVGAPL
                     STVGGLLVLAVTGLPLDISSMTGLILLVGLVVKNGILLLEHAQRGIASGQPFDAALVA
                     ATRRRLRPILMTTAATIAGLAPLAFGIGAGAELQRPLAVAVIGGLAFATAVTLFVTPG
                     LASLLQRHRA"
BASE COUNT         1077 a         2017 c         2194 g         1081 t
ORIGIN      
        1 caatcactgc gtccatgcgc gttgttctcg cgttcctctt cgccgcgtgt gcggatccga
       61 caccgcgcaa ggttcaactg atcgacgccc cgccggcagg ggaggtcgag ccgatcatca
      121 gcggcgagct cgcacaggcg atcgccgcaa acgattcgtt gctggtctac gtcggcgcga
      181 gctggtgcga gccatgccgg cactttcacg aggccgccga ggccgggcag ctcgacgaca
      241 agcttggcgg cttgcgcctc ctcgtgttcg acgccgatcg cgacaccgac gcgctcgaga
      301 gcgccggtta ccactcgaac ttgatcccgc tgttcgcggt gccgcgcatc gacgggcgcg
      361 cgagcggtca tcagatcgaa ggtgcgatca agaatcgcga cgcggtcacg cagctcgtgc
      421 cccgcatcaa gacgttgctc gcgcgctagc ggaccgcttc gtcgagcacc gcaaacgcga
      481 ggtgcgtgac gcgggcgaga tgatcgaact gcacgttcgc gtaggtgtcg gtcgacttgt
      541 gatagttcgg cgtgtgatcg ccgccgacga actcttcgct gacgccagtc gcggggagag
      601 cggcctcgac gaacgcttgg tgatcgctga gctcgaacgt gaccggctgg atcggcatgc
      661 cggcgaccgc acccttcgct tgatacattg cctcgaccgc gggagccggt gaccacagct
      721 cgactgcgtg atcgccgtcg ccgtcgaagc tgaccatgtc gtagatgtgc acgcttgcga
      781 tgtcgccagc ggtgagcgtc ttgacgtact cgcggctgcc gataagcccg agctcttctt
      841 gatcgaacag cgcgaatgcg atcttgtgcg tgcgcggtac gtcgcgcagg tagcgggcga
      901 gcgatagcac gatcgcgacg cccgtcgcgt tgtcggcggc agctgggcca gcagccacgc
      961 cgtcgaagtg cgcaccgatg atgatgagcg agcccgggcc gtcgagcgtg ccgatgacgt
     1021 tcgcgccgac gttgctgccc gaggtgtagt tctgatgttg cgacggaatg ttcagcgcag
     1081 cgaacgcctc gtcgaggaac tgccgggtcg cgttgcgctc gccgaccgac tcgcgatgcg
     1141 cgatcttgac gccgggcgag atgtcctcga cgcccgcgag cacgccgaca atgtggcgtt
     1201 gatagctgtc gagccagtcg cacgtgccaa gcggcgtgca gaccggggcg gcgtcgatca
     1261 tcggcgcctt cggcgcgtcg atcatcggct gcgtgtcatc gccgctctgc gacatgctgc
     1321 tgccattaca tgcagcgagc agagcgacca ggccgaggcg acgcatgcgg gcaacctacg
     1381 tcaggcggcc cgcctccggc ggaaaacccg cttacacacg ttcaccttgg tgcgtatcgc
     1441 tagaagcccg ccggcccaag agccgttggg atacaatggt tcgtccatga cgacccgcta
     1501 cacggtgctc gagcgactcg gtggtggtgg ccaagccgag gtgtttcgcg gcgtgtcgga
     1561 gtcgatccag ggcttcaaga agccggtcgc gatcaagcgc gtgctgccgc acctgacgag
     1621 caacccgcag ttcgtcgcga tgttcctcga tgaagcgcgc ctatcgctgt tcctgcagca
     1681 cgcgaacgtc gtccaggtct tcgacatctc caaggcagcc gatggcacgt acttcctcgt
     1741 catggagttc gtcgacggct gcgatctgaa ggggctgatc catcaccagc gcacggtcga
     1801 catcggcctg acgctgtaca tgatgatcga agccgccaaa ggcctgcatt acgcgcacac
     1861 gctcgagcat ccgcagaccg gcaagccgct cggcatcgtc caccgcgacg tgtcgccacc
     1921 gaacatcatg ctgtcgaaga acggcgaggt gaaggtcgtc gacttcggtc tcgcgaaggc
     1981 ggagagccag gtcgagatca cggacgaagg cgtcgtgaag ggcaagttcg cgtacctggc
     2041 gcccgaggcc gcgttcggta aggaagtcga tgcgcgcgcc gacgtcttcg cgctcggcat
     2101 catcacctgg gagatgctga cggggcgccg cctgttctac aacgacgacc cgcacaagac
     2161 gatcgagctc gtgcgagcag ctcgcgttcc gtcgatcacc gcgctcaatc ctaaggtgga
     2221 tcccgagctc gatgccatcg tgcgcaaggc gctcgcgcgc catcccgaga atcgcacggc
     2281 cagcgcagcg gactttgccg acgagctcgc ccagcatctg ttcggtaacg agatgaaggt
     2341 cacgtctcgc aatgtcgccg cggcggtgca agcgattcgc gcagcgaaga agccatcgtc
     2401 ggcggcgaag gcgctgatcg atgcgttgat cgctgacgaa gtcgcgaaga tgacgtcgat
     2461 cgtcgacaca caggctgccg aggatgctcc gaagccggcg gagatcgtcg acaccgccga
     2521 ctgggcgtcc gagctgatcg acaagtagca gcacgtacgc gcgacgctga cgctgtgcat
     2581 gtcgattgca acctctcctc accgaggaga aatcgtcatg gctacttcgt cgtcaatcga
     2641 gggtcgcggc cgccacgaaa gcaggtactg gggctggccg atggtgatcg gcatcttgct
     2701 gatcatcggc ggcctgttcg cgctgtacgc gtcggtactc acgagcttca tctcggtcat
     2761 ctatctcggc gtcatgctga tggtcgtcgg cgtgctcgag gtgatctccg gcttccgcat
     2821 gcgtcacacg cagccgttcc tcgtctacct gctcgcgggt ctgctcgcga tcgtcgtcgg
     2881 cttcatgttc ctcgaccagc cgaaggtcgg ggtcctggcg atgagcctgc tcatctgcgg
     2941 ctatctgttc gcgagcggtc tgttccgcgg cgtcgtcgcg attgctgagc gctacccgcg
     3001 ctggggctgg gacctcgcgt acggcatcgt cgcgatcctg cttggcatct atctgctgaa
     3061 ggagtggccg atcgcgtcgt tctggttgct cggtaccctc gtcgcgatcg agatcatcgc
     3121 tcgcggtgtc tcgctggtcg ctgcctcgtg ggtgatccgc gacatccagc accaccgcct
     3181 ggcacacggc ttcgcctaga gcgacgccgg atacgtatcc tcgatctcga ggagtcggcc
     3241 ggccttcgga tcgcacaccc acgcgtggtt gcgatcgtcg gccgtgacgc agtgcgcgcc
     3301 gagtgcggcc tccgcggtac cgagtagcga gaacttgccg cccgcggcga cgccgatgat
     3361 cgagaacgtc gcggtcttcg cgctcggcac gtagaggtgc gagagcttcg ggttgaagtc
     3421 gatgatatcg acgccggcgc cgacgacgat cacgtcgagc tgcttgccgg agctcgcgtc
     3481 gagcgcgacc gcgcgacctt cgccgcagcc gacaaacaag atgttatgcg cgccgtcgag
     3541 cgcgataccg cgcgagccgg tgcagccgtt tggccacgac gcggtttgct tgcgcgtggt
     3601 gacatcgagc acgaccgtcg tgcctttcca caggtgcgtg aacgcgcgct tgccgtcgat
     3661 cacgagcgat tcgggtccgc ctgtcaccgc gatgaacgcg gcgtgcttcg gtgtcgtgcc
     3721 cgtcagcgtg aatacctcga tgcgatactc gtcgggctgg gtgacccaca gctcgtgcgt
     3781 agcctcgacc cagcgcacgt agtcaggcga gctcgcgagc ttcgccgagc cgacgatctt
     3841 tgcggtcttc tcgtcgatca ggacgacctc gagcttcgtg cgatcgatcg cgtacagatg
     3901 gccggcactc tcgtctgccg acgtcgagcc atcctcgtgc cccgtcgttc cggtcgtcgc
     3961 gccgaagccg gcgatcgtcg tcagtgcacg cgtggcagga tcgatcagag cgatcgtacc
     4021 ggtgccgccc gcgggcgcga cgacgcgacc gatgccgcgc gcgaaccgca gatcgtcgaa
     4081 gccgatgccg tgctcgccgc ccggaatggc gaccggggtc gcagtggacg tggcggcgtc
     4141 ggccgacgcg gtggctacgg tcggcgttcc ggcctcggtg gccggtggcg tggtggtggc
     4201 aggctcactc gagcgcgacg cgcccttgca cgaggcgacc gcgcacagcg cgaacaggac
     4261 gtttcgcatg gcgcgtcgat aacacagctc aggcgcgatg gcgctggagc agtgacgcga
     4321 gaccgggcgt cacgaacagc gtgaccgcag tcgcgaaggc gaggccgccg atcacggcga
     4381 cggcgagcgg gcgctggagc tcggcgccgg caccgatgcc gaacgcgagt ggtgcgaggc
     4441 cggcgatcgt ggcagccgtc gtcatcagga tcggacgcag acggcggcgc gttgcggcga
     4501 cgagtgcggc atcgaagggc tggccgctcg cgatcccgcg ttgtgcgtgc tcgaggagca
     4561 agatgccgtt cttgacgaca agaccgacga gcaaaatgag cccggtcatc gacgagatgt
     4621 cgagcggtaa gccggtgact gccagcacga gcaggccacc gacggtcgag agcggcgcac
     4681 cgacaagtac gacgagtgcg aagcggagcg agtggagctg gagcaccagc acgaacagca
     4741 cgaggagggt ggcggtgagc gcgatgacgt agagctcgcg gcgcgcggcc cgtgcggttt
     4801 cggcttggcc gccgatcgcg atctgcacac cgggcggtgg ttggacgtcg tggagcgcgg
     4861 cgacgactgc ttgctcggcg ccgccgaggt caccgtcttt tgcggcagcg gtcatcacga
     4921 cggccgcacg cagaccgtca cgtttgagga ccgcaggcgc gagcggacga tcgaacgtga
     4981 tcacttcgcc cagcgctagc gattgcgggc cgtacgcgat cgacgaacgc gcgagtgccg
     5041 ttgggtcgtt gcggatcgcg tcggggtaac gcaggcggac gccgatcgcg cgctcgggac
     5101 gcagcacttc gccgacgacg cggccctcga gtgcgatcgt cagatcggtg ccgatcgtgg
     5161 cagcgtcgag gttcaagcgc gcgatctggc cgagctcgag ctggccgcgc aggatcggcg
     5221 cgtagccctc gcggccatcg aagaagtctt cgagctcgtc gagcttggcg aggcgctcgc
     5281 cggcttgctc cgcgtagctt tcgagctgac gcggatcgtt gccgaggatg cgaacctcga
     5341 tcggcgcagg attgcctgcc aggtcggcga gcacgtcttg caggacctgg atgtactcga
     5401 aacgcgattc cggaacgcgg tcgtgcagct tgtcgcgcac atggtcgatc acgtcgtcga
     5461 tcggtggccg atcgccgggc ggcacgagac gcaccatgat gtcacccgtg ttctgcaacg
     5521 tcgcggtcgc cgggccgagc tcggtgccat tgcggcgcgc gaacgacgag acctccttgg
     5581 tctcgcgcaa cacatcgtcg atcgccgcgg tgatgcgtgc cgattcttcg agcgaggtgc
     5641 cgggtgggag cgcaaagtcg atgacgaatg cgccctcgtc catgtgcggc aagaagccgg
     5701 tcggggtgat gcgggtcgca ccaaagccgg cgccgatcaa aaacacgatc acgagcaccg
     5761 cgacgaggcg atggcgcagc agccacgcga tggtgcgcga aatgcgatcc ggcttgtcgt
     5821 cgcgttcgac acgcttgcga gcgtgtaggc ggtgcgcgag caccgggatc agcgtgagtg
     5881 ccatcagcat cgacagcagg actgcgatcg cgagcgtgag tgcgagcgca ccgaggaagc
     5941 tgccggtggt gccgctgagc agcgcgagcg gcgcgaacac gacgacggtc gtgagcgtgg
     6001 tgccgatgac ggggccgagc atgtccgagg tgccgagctc ggcggcttct gcgggcgtgt
     6061 ggccttcttc gagtcggcgc acgatgcctt cggtaacaac gatcgcgtcg tcgacgacga
     6121 ggccgatcga gatcgcgagg ccgccgagtg acatgagatt gagcgtcatc ccgaatgccc
     6181 gcatgacggc gaacgtgccg agcaacgtga tcggcaccgg cagcgccgcg atgaggccgg
     6241 cgcgccagtc gcgcagcgag atcgcgatca cgatcagcga cagcaggatg ccgatcaaga
     6301 tcgcgtcacg cacgcttgcg agtgattcgt cgacgagagt tgcctgatca tagaccggct
     6361 cgatgtcga
//
LOCUS       DASUFX010000638        16127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_380183,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000638 DASUFX010000000
VERSION     DASUFX010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16127
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380183"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..852)
                     /locus_tag="VFV99_25710"
     CDS             complement(100..852)
                     /locus_tag="VFV99_25710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5062806.1"
                     /translation="MDVTHAVLIGAAAFAAGAVNAIAGGGSLITFPVLLASGLPAVTA
                     SMTNTVAMCPGYFGAVIAQRRDLAGQGSRAVRLVPLSLVGSLAGAWILLQTTEKSFQT
                     IVPFLLVFAAGLLAAQGKLRRYLAQHTRGGHSMAFAILPVALSAIYGAYFGAGMGVII
                     LAILAVVVDDTLLRANALKQLLSLIINTCAAVVFLFVGKVEWSVAAIIAVTSLIGGLV
                     GGRFVTRIPEALLRWVAITVALAVAVVYFAKL"
     gene            complement(862..1941)
                     /locus_tag="VFV99_25715"
     CDS             complement(862..1941)
                     /locus_tag="VFV99_25715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5062807.1"
                     /translation="MAVIGQGHFAQAAVLPAIEQLDDVELTALVSGSDHKLHELGERY
                     GVRTLVHYDDLDQLLASGKVDAVYIVVPNDMHAEMALIAARHGVHVLCEKPMAPTEAE
                     CMQMIRACEQRHVKLMIAYRLHFQAANLVAIELARGGEIGDPRVFSSTFSMQVREGNT
                     RVQARRGAGPLYDIGTYCVNAARYLFRAEPLEVIGLKLVGRDHRFAAVEEAFAVTMRF
                     PQERVAQFTCSYSAHDRAHYELIGTDGVLTLDNAYEYALEMTMRVEGRHGIKTRTFEK
                     RDQIAGEIEYFVRCVRDNVDPEPSGWEGLADVRILQAIQQSARFGRAVSIDPVLPPRR
                     PTLGQEIDVDPHPAPQLVDVQQPTK"
     gene            complement(2356..2742)
                     /locus_tag="VFV99_25720"
     CDS             complement(2356..2742)
                     /locus_tag="VFV99_25720"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5062808.1"
                     /translation="MEHALLPYVTMQKFRRTLGATTLIHDRVVNLNGEYIGKIDELMV
                     DVTTGRVAYAVLSFGGFLGFRRKLFALPWSALTVDEAHKRFVCNVTKEALDRMPGFDK
                     EHWPDLNDLEYATGVYRQWGATPYWT"
     gene            2798..3037
                     /locus_tag="VFV99_25725"
     CDS             2798..3037
                     /locus_tag="VFV99_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062809.1"
                     /translation="MARGSKKSYTSKQKRQAHHIEESAKKRGYSSKRAAQIGYATVNK
                     QDKGGKKSGSGRGKTRSTASSRKGGRKGGRSRKSR"
     gene            complement(3038..4507)
                     /locus_tag="VFV99_25730"
     CDS             complement(3038..4507)
                     /locus_tag="VFV99_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX5062810.1"
                     /translation="MWQPPERIKHELPGTSWPTAEMAAQARMFQPLAIGPRTAKQRTW
                     VPAMVPWRSTEEGIVTPDVIDWYRRFAEGRPGVIVVEATGIREIPSGPLLRIGADRFI
                     PGLRDLVAAVKEASGGETLLFIQLIDFLSIRRRPPRDKYFDRFLALTSRHREALAARG
                     DAAALTCSDAELRGKLAALKDSELPTVLTAREVEDLERGFRERVTDMHIPHIAELPQV
                     LPTMFADAAGRALEAGFDGVELHFAHAYTMASFLSALNTREDGYGGPRENRVRLPREV
                     IAAVRAKIAGRAVVGCRYLGDDVIEGGNRVDDAAWFGVELARAGLDFLSISKGGKFED
                     AKLPKVGWAVYPYTGKSGYECMPTIYSDAKGPFLRNVSLAATIRHAVRDAGFTTPVVT
                     AGGICDFTEAEAVLERGDADFIASARQTLADPDWFAKIRTGRGGEIRRCEFTNYCEAL
                     DQQHKQVTCKLWDRDELDEPDVTLASDGKRRLVAPRWRR"
     gene            4536..5825
                     /locus_tag="VFV99_25735"
     CDS             4536..5825
                     /locus_tag="VFV99_25735"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5062811.1"
                     /translation="MEPFEPHLGLLTALAVGLLIGLEREQAKADAEAAADPAKGKTLG
                     GFRTYPIIALTGAVATMLEGASMWLPLVALGGLVALLAISYAGDVRRDADHGMTTEAS
                     AVVTYLIGALATSRGVVEPMATRLLLVVALGVTITLLLSAKKWLHTFATRVSREDLFS
                     TLKFLIVAAIVLPLLPRADMGPLDAINPFSVGLMVVLIAGLSFLGYVAMKLYGERWGV
                     LLSAAIGGLVSSTAVTIAFANRTKEDPGLAPVAATAIAIASTIMVARVAVLVGIVKPE
                     LLPELWLPLAGAAAGAILGGLVGYHGTKKQRGEEMKIKNPFELGSAVRFGIVFAVILL
                     AVKAARYYLGDRGLFIAAGIGGLTDVDAVSLSTAKQAAPQDAAAVVAIMIAVASNTIV
                     KSSLALGIGGRRLGKRAFLIGGLMVVGAALGLIPVVV"
     gene            complement(5822..6307)
                     /locus_tag="VFV99_25740"
     CDS             complement(5822..6307)
                     /locus_tag="VFV99_25740"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX5062812.1"
                     /translation="MDKQQLIAQLVKQLESSARSALSARDAAAQEAREGATPDEKRED
                     ARAAHQLQTLGAAQQRRAQQALADVDALARFKPGKMATIGVGAIVEIEDEDTGEGRTF
                     FLSPVGAGMTLSGPGGDGVLSVVTPASPIGKAVLGRRVGDVVDITVDGDVREWQISFV
                     S"
     gene            complement(6347..6649)
                     /locus_tag="VFV99_25745"
     CDS             complement(6347..6649)
                     /locus_tag="VFV99_25745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062813.1"
                     /translation="MADNEDAAARIAALEQENAELRAKLAERESRRPESRPQVELISR
                     DDAKRSRFAKLMLAGGVVATLGGIFGASWFAVICGLGWLLGGAAIASTVPQSGGES"
     gene            6673..7122
                     /locus_tag="VFV99_25750"
     CDS             6673..7122
                     /locus_tag="VFV99_25750"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5062814.1"
                     /translation="MLKVGDPMPDVTLVGETGPVQLRSFIGKPAVVYFYPKDETYGCT
                     KEACSFRDSYEDFVGAGAQVIGVSRDDAASHASFKQHHRLPFTLLSDPGGQLAKAWGV
                     KGGLGLPGRVTFVFDKDGVCRHRFDSQIRFGKHVDEALEVVKQLAAA"
     gene            7091..8392
                     /locus_tag="VFV99_25755"
     CDS             7091..8392
                     /locus_tag="VFV99_25755"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5062815.1"
                     /translation="MKSSSNSPPLSASPPAASPPHGASLARVLGLPGAVLMGLGSIVG
                     TGIFVSIGTAAGVAGSAVLVAVGLAAIVATCNGLSSAQLAAAHPVSGGVYEQAYRYAT
                     PTLGRIAGFAFVSAKTASAATAALGCAGYALALASSGDRTVQIVVAAMVVVVLTIFVA
                     GGMQRSSRLNAVVVALTLVSLLVFVVAGLPHVDVARLNPTGYAPSARAPGADDMQLGS
                     LVRDIAHATALMFVAYTGYGRIATLGEEVREPARTIPRAIIVTLVVALLLYLAVTTVA
                     IGSVGADGLAAATRAGSAPLETIAAGFGEPIRYIVAAGAITAMLGVLLNLLLGISRVV
                     LAMARRRDLPHGLATVHGSSPRRAVIVTGVAIAALALIGSVKTTWSVSAFFVLVYYGI
                     ANYCALRQPASERRFTRAVPVLGLVSCLALAVWLVIDVLSP"
     gene            complement(8402..9799)
                     /locus_tag="VFV99_25760"
     CDS             complement(8402..9799)
                     /locus_tag="VFV99_25760"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5062816.1"
                     /translation="MYNKIPQWYALATVAYLVAAVLFILALRGLSSQETARRGNVYGI
                     VGMAIAIGATLSFRGANFHPLLLGSIVIAIVIGATMALRVGMTQMPEMVALLHSFVGL
                     AAVLVGFSLQLHLGTFLPMAEKVEIYVDVIIGAITTTGSVLAFLKLRGSVSGKPLLLP
                     ARHFANLALLIGIVTLGALYGEKSETWKLLVASGLAGVLGMHLVAAIGGADMPVVVSM
                     LNSYSGWTAAAAGFMLHNDLLIITGALVGSSGAILSYIMCRAMNRSIWNVIFGGFGAA
                     PPKAAAKGPAQPAGEVQEIDAPTLAATLKGSKNVIIVPGFGMAAARAQHAVREMTDVL
                     RKDGVNVRFAIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEINHDFPQTDVAIVIGAN
                     DIVNPAAEEDPTSPIAGMPVLQVWKAKLVVVNKRSRAAGYAGIDNPLFYKPGTKMLFG
                     DSKDAIEKVVSALKG"
     gene            complement(9808..11421)
                     /locus_tag="VFV99_25765"
     CDS             complement(9808..11421)
                     /locus_tag="VFV99_25765"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX5062817.1"
                     /translation="MKLGIVKETRPLERRVAASPNVVGKWVKAGWQVEVERGAGATAS
                     YPDSLYEMAGATMVDRLTAWRADIVLKVRPPELNEVDQLRDGGTLISFIYPAQSEALV
                     AKLAAKKLTVLAMDQVPRISRAQKMDALSSMQNISGYRAVIEAANRFGSFFTGQFTAA
                     GKVPPGKVLVIGAGVAGLAALGAAKGLGAIVRAFDVRAAAEEQVKSMGAEFLTVKIKE
                     AGEGQGGYAKEMSKEFIDAEYALFREQAKDVDVVITTALIPGKPAPKLWLKDMVDMMK
                     PGSVVVDLAAEQGGNCEYTKPGEAIDQNGVTIIGYTDLPSRMAHIASDLYGMNLWHFL
                     DEMGGAAKYKIDHENDAVRPALVLENGEKRWPAPPMKKAEAPVAPPPKPEPPKPEAKP
                     AVSHPPKPSGVHGHGAATSPPTRAGGIIMSLIGILAAIAWLSLRFAKPDAMGSAETTE
                     LIQRFTVFVMAVFIGFQVVWNVTPALHTPLMSVTNAISGIIVVGGLLAGSGKLTSSVI
                     VAVIATLFATINIAGGFIVTRRMLAMFRK"
     gene            11485..12441
                     /locus_tag="VFV99_25770"
     CDS             11485..12441
                     /locus_tag="VFV99_25770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062818.1"
                     /translation="MIEVAGLTVHQDVVEHPLLGDAHIIEHAGEPITAMSAIDWDRPT
                     RIPTVAEPRALPRGTGSMLINEIASRAQRAGVQALRYAGPYPTPALFNTLKRSFRTTA
                     TESIFSSDVLGRAMRLARDEVPVDFVPAPFTRQPMPYGSIDVRDGIERAVINGILFDQ
                     EPTVGSLARLVGIEAPIGSTASGAAAVLAFDTVVWTHVAELATDGSLVGDVRPIPALD
                     SNVIGKQFPAELKQIFSEFVASSDAIPGPLCRDTERVLSERLITWRDLGWRAAAREGD
                     GFALHVGWWTHLVPRGFETLVRALADAIAPVAVQLILDEVTR"
     gene            complement(12467..13174)
                     /locus_tag="VFV99_25775"
     CDS             complement(12467..13174)
                     /locus_tag="VFV99_25775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062819.1"
                     /translation="MTAAALVTPARAQDAFEIQVYASDTAARGEAGLEVHANHHFVRD
                     AADASHLTFEPHYGVLEWLEVGGYLQTALTDGDVAFGGGKLRVKARMPRRLWHDRIGL
                     AVNTEVSAVPEQFEPDVWGSEVRPIADLTTTDFYASINPILAIDLRGELAGHPQLEPA
                     VKLAGRLSPRWSLGVEAYGAFGPIDDLGSEHVGTLLAAVDYAGRWLDLNVGAGPSWGG
                     DDGAIIKIIVGVHPSSE"
     gene            complement(13274..14098)
                     /gene="aat"
                     /locus_tag="VFV99_25780"
     CDS             complement(13274..14098)
                     /gene="aat"
                     /locus_tag="VFV99_25780"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5062820.1"
                     /translation="MPVFRLDDRLVFPPVHLAEDGLLALGGDLRPERLLLGYTQGIFP
                     WYAENLPILWHSPDPRMVMTTRDLIVQRSLRKAIKRRPYELTTDKAFEEVLRGCANVP
                     RPGQTGTWLIPEMVDAYKKLHELGFAHSFEAWKGGELVGGLYGVSLGAAFFGESMFAK
                     APDASKIAFAASVRQLDSWHIGLIDCQVHTEHLERFGAYEIPRLEYLQMLARALDEPT
                     KRGKWTMDIDFDQFAATGGMMIAANGHAPNGANGANGANGANGANGANGANGTHDE"
     gene            14128..>16127
                     /locus_tag="VFV99_25785"
     CDS             14128..>16127
                     /locus_tag="VFV99_25785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062821.1"
                     /translation="MKWFVIAVAILAGCAPVAPRFPTDVQTALSADMRRSEMRRLETE
                     RFIIYYPGWNRFGVERLLERADRCLQTLREHALIKTTKKIVMLMPDVPFNNAFVMPDA
                     LGYEQISVIPTHFTLDFATAFGLPPDPGAVACHELTHYVHFEQTAGFWGTFNEWFGPM
                     YTPQLGYDPWFDEGIATHYEARLSPGLGRPVWPLFTGMFAAAYAGRDINSGELSSFAR
                     EASVGHHYLVGSMFVRFLVEKYGERPLWTTIENQAHAFTGWFFTGTFKHGFAVSFSDL
                     FAQFGRWVHETFPVRVRPPTQRKLATIGNDARYARGRDGTEAWVAEDVDSPPRLTVRD
                     AHGATLLDTAITEVVPPRTVANADPLLVSGLSITADGNEVWLTAIDLGSTYQVTRLLR
                     WRRGERGLTEVAHDLGPGATIDPTGSTYYYCQVSGSHWSLLAYDVKRGVTDRIVMDMN
                     SETGVYVLGAQVSPDGKQLAANVWNGSAFVIWIVDAQHGVFAREIKGNGTPVYDASFT
                     ADGRVMYLGVVDGRFQAFIEGTQVTDAPYAVLAARDAGGTIRFLDREHWEWELAEVAT
                     PPKPAPTPPTPPTPPTPPTPPTPPTPPEGTPSPDAAPTTDAPQPAAGQPPAAQPATGQ
                     PVTGQPPTGQPVTGQPPTGQPPTGQPPTGQPATGQPATGQLAT"
BASE COUNT         2709 a         5455 c         5206 g         2757 t
ORIGIN      
        1 gacgacgccg gtatcgctgg ggccgtgctc gccgaagcca aaccggccgc agctagcgag
       61 cagcaggatg acgacagccc gcacactcgt agtgtcgcgc tagagcttcg cgaagtacac
      121 aactgcgacg gcgagcgcga ccgtaatcgc gacccagcgc agtaacgcct cgggaattcg
      181 ggtgacgaac ctgccgccga ccagaccgcc gatgagtgac gtgaccgcaa tgattgcggc
      241 gaccgaccac tcgaccttgc cgacgaacag gaacacgacc gcggcgcacg tgttgatgat
      301 gagcgacaac agttgcttga gcgcgttcgc gcgtagcagc gtgtcatcga ccaccaccgc
      361 gaggatggcg agaatgatca cgcccatacc ggcgccgaag tacgcgccgt agatcgcgga
      421 cagcgcgacc ggcaggatcg cgaacgccat cgagtgaccg ccacgcgtgt gctgcgcgag
      481 gtagcggcgc agcttgccct gcgcggcgag caggcctgcg gcgaacacca gtaggaacgg
      541 cacgatcgtc tggaacgatt tctcggtcgt ctgcagcaga atccacgcgc ctgcgaggct
      601 gccgaccaga ctgagcggca cgaggcgaac cgcgcggctc ccctgccctg cgagatcgcg
      661 acgctgcgcg atcacggcgc cgaagtaccc cgggcacatc gcgaccgtgt tcgtcatgct
      721 cgcggtcacc gccggcaagc ccgacgcgag cagcaccgga aacgtgatga ggctaccgcc
      781 acccgcgatc gcgttgaccg cgcctgccgc aaaggcagcg gcgccgatca agaccgcgtg
      841 ggtgacatcc acccaacaac actacttcgt cggctgctgg acgtcgacga gctgcggcgc
      901 gggatgagga tcgacgtcga tctcctggcc gagcgtgggg cggcggggcg gcagcacggg
      961 gtcgatcgag accgcgcggc caaagcgtgc cgattgctgg atcgcctgga ggatgcgcac
     1021 gtccgcgaga ccctcccaac cgctgggctc tgggtcgacg ttgtcgcgca cgcagcgcac
     1081 gaagtattcg atctccccag cgatctgatc gcgcttctcg aatgtccgcg tcttgatgcc
     1141 gtggcggccc tcgacgcgca tcgtcatctc tagtgcgtac tcgtatgcgt tgtcgagcgt
     1201 cagcactcca tcggtaccga tcagctcgta gtgcgcgcga tcgtgagcgc tgtagctgca
     1261 cgtgaactgt gcgacgcgtt cctgaggaaa tcgcatcgtc acggcaaacg cctcttcgac
     1321 cgcagcgaag cgatggtcgc gacccacgag cttgagcccg atcacctcca ggggttctgc
     1381 acggaacaga tagcgcgccg cgttcacgca gtaggtaccg atgtcgtaca gcgggcccgc
     1441 gccccgccgc gcttgcacgc gcgtgttgcc ctcgcgcacc tgcatcgaga acgtcgagct
     1501 gaacacgcgc ggatcaccga tctcgccacc gcgcgcaagc tcgatcgcga cgaggttcgc
     1561 agcctggaag tgcagccgat aggcgatcat caacttcacg tggcgctgct cgcaggcgcg
     1621 gatcatctgc atgcactcgg cttcggtcgg tgccatcggc ttctcgcaga gcacgtgcac
     1681 gccgtggcgg gcagcgatca gcgccatctc tgcgtgcatg tcgtttggca ccacgatgta
     1741 cacggcatcg accttgccgc tcgcgagtag ctggtcgaga tcgtcgtagt gaacgagcgt
     1801 gcgcacgccg tatctctcgc cgagctcgtg cagcttgtga tcggatcccg agaccagcgc
     1861 agtgagctcg acatcgtcga gctgttcgat cgcggggagc accgccgcct gcgcgaaatg
     1921 cccttgcccg atcactgcca ttctcagcct tgcgcgactc atatccgtcg aaaacagcaa
     1981 tccgcgttcc gcgtatgcgc tacgtacggc tcgacccttg cagcctcact gggcccatga
     2041 agccttgtga aacctgcgga aacgtctacg acaaggcgtt cgaagtgaag atcggtggca
     2101 ctactcacac cttcgattcg ttcgcctgcg cgatccatat gctcgcgcca cgttgcgggc
     2161 attgccagac accgatcatc ggtcacggca tggaagcgcg cggacagtat tattgctgtg
     2221 catcgtgcgc acgcgtggcc gggatcgatg agctaaccga tcgcgctccg gatcgcggtc
     2281 aggctgcgcc gtagtagcac gcacgttctc gtcacgccgt gcgcatgcgc gcgcgcgact
     2341 ctcacgtcac gtccgtcacg tccagtatgg cgtcgcgccc cactggcgat atacgccggt
     2401 tgcgtactcg agatcgttga gatctggcca atgctccttg tcgaagcctg gcatgcggtc
     2461 gagcgcttcc ttcgtgacgt tgcagacgaa gcgcttgtgg gcctcatcaa ccgtcagcgc
     2521 gctccacggt agcgcgaaca gttttctgcg gaagcccaag aaaccgccga acgagagcac
     2581 tgcgtaagct acgcgacccg tcgttacatc aaccatgagc tcgtcgatct tgccgatgta
     2641 ttcgccgttc aggttcacga ctctgtcgtg gatcagagtg gttgctccta gagtccgtcg
     2701 aaacttctgc atagtcacgt atggcagcaa tgcgtgttcc atacgaattc ctcgacgggc
     2761 acatggttcg catacagggc gcgcctagga ggaactcatg gcccgaggca gcaagaagag
     2821 ctacacgagc aagcaaaagc gacaagcgca tcacatcgag gagagcgcga agaagcgcgg
     2881 ctattcgtcg aaacgcgcgg ctcagatcgg ctacgccact gtcaacaagc aggacaaggg
     2941 cggcaagaag agcggcagcg ggcgcggcaa gacgcgtagt acggcatcga gtcgcaaggg
     3001 tggtcgcaaa ggcggccgct cgcgcaagag ccgttagcta acggcgccag cggggcgcga
     3061 cgagccggcg cttgccgtcg ctcgcgagcg taacgtcggg ctcgtcgagc tcgtcgcgat
     3121 cccagagctt gcacgtgacc tgcttgtgct gctggtcgag tgcttcgcag tagttcgtga
     3181 actcgcagcg acgaatctcg ccaccccggc cggtgcgaat cttcgcgaac cagtccggat
     3241 cggcgagcgt ctgccgcgcg gacgcgatga agtcggcgtc gccgcgctcg agcaccgcct
     3301 cggcttcggt gaagtcacag atgccaccgg cggtgacgac cggcgtcgtg aaaccggcgt
     3361 cgcgcacggc atggcggatc gtcgccgcga gcgacacgtt gcgcagaaac ggtcccttcg
     3421 cgtccgagta gatcgtcggc atgcactcgt agccggactt gccggtgtac ggatagaccg
     3481 cccagccgac cttcggcagc ttcgcgtctt cgaacttgcc gcccttcgag atcgagagga
     3541 agtcgagacc tgcgcgcgcg agctcgacgc cgaaccacgc cgcgtcgtcg acgcgattgc
     3601 cgccttcgat cacgtcgtcg cccaagtagc ggcacccgac gacggcgcga cctgcgatct
     3661 tcgcgcgcac cgcggcaatg acctcgcgcg gcagccgtac gcgattctcg cgcggtccgc
     3721 cgtagccgtc ctcgcgcgtg ttcagcgccg acaggaacga cgccatcgtg tacgcgtgag
     3781 cgaaatgcag ctcgacaccg tcgaagccag cttcgagtgc gcggcctgcg gcatcggcaa
     3841 acatcgtcgg cagcacctgc ggcagctccg cgatgtgcgg gatgtgcatg tcggtgacgc
     3901 gctcgcggaa cccgcgctcg agatcttcga cctcgcgtgc cgtgagcacc gtcggcagct
     3961 cgctgtcttt cagcgccgcg agcttgccgc gcagctcggc gtcgctgcag gtgagcgctg
     4021 cggcatcgcc gcgtgcggcg agcgcctcgc gatgccgtga cgtgagcgcg aggaaccgat
     4081 cgaagtactt gtcgcgcggc gggcgccggc gaatcgacaa gaagtcgatc agctggatga
     4141 acagcagcgt ctcgccaccg gatgcttctt tgacggcggc gacgagatcg cgcaggcccg
     4201 gaatgaagcg atcggcgccg atgcgcagca gcgggcccga cgggatctcg cggataccgg
     4261 tcgcctcgac gacgatcacc cccggcctgc cctcggcgaa tcggcggtac cagtcgatga
     4321 cgtcgggggt gacgatgccc tcttcggtcg agcgccacgg caccatcgcg ggtacccagg
     4381 tgcgttgctt cgcggtccgc ggcccgatcg cgagcggctg aaacatccgc gcttgcgcgg
     4441 ccatctcggc ggtcggccac gacgtgcccg gaagctcgtg cttaatacgc tccggtggct
     4501 gccacatggc cgccgattcg taccacactt gcgacgtgga gccgttcgag cctcacctcg
     4561 gtttgctgac ggcactcgcc gtcggcttgc tgatcgggct cgaacgcgag caggcgaagg
     4621 cagatgccga ggccgccgcg gacccggcga agggcaaaac gctcggcggc ttcagaacct
     4681 atccgatcat cgcgctgacc ggcgcggtcg cgaccatgct cgagggcgcg tcgatgtggc
     4741 tgccactcgt cgcactcggc ggtctggtcg cgctactcgc gatctcgtac gccggagatg
     4801 ttcgcaggga cgccgaccac ggcatgacca cggaagcctc ggcggtcgtg acgtatctga
     4861 tcggtgcgct cgcaacaagc cgcggtgtgg tcgagccgat ggcgacacgg ctgctgctcg
     4921 tcgtcgcgct cggcgtcacg atcacgttgc tcctgtccgc gaagaaatgg ctgcacacgt
     4981 tcgcgacaag ggtctcgcgc gaggacctgt tctcgacact caagtttctc atcgtggccg
     5041 cgatcgtgtt gccgctgttg ccgcgtgcgg acatgggacc gctcgacgcg atcaatccgt
     5101 tctcggtcgg cctgatggtt gtcttgatcg ccggtctgtc gttcctcggc tatgtcgcga
     5161 tgaagctgta cggcgaacgc tggggcgtgt tgctcagcgc agcgatcggc ggcctggtgt
     5221 cgtcgacggc ggtcacgatc gcgttcgcga atcgcacgaa ggaagatccc gggctcgcgc
     5281 cggttgcggc gacagcgatc gcgatcgcgt cgacgatcat ggtcgcgcgt gtcgccgtgc
     5341 tcgtcggtat cgtcaagccg gagctcctac ctgagctgtg gctgccgctt gcgggtgctg
     5401 ccgccggcgc gattctcggc ggtctcgtcg gttatcacgg cacgaagaag cagcgcggcg
     5461 aggagatgaa gatcaagaac ccgttcgagc tcggcagcgc agtgcgcttc ggcatcgtgt
     5521 tcgccgtgat cctgctcgcg gtgaaagcgg cgcgctacta cctcggtgac cggggcttgt
     5581 tcatcgcggc cggcatcggc ggcctcaccg atgtcgatgc ggtctcgcta tcgacggcga
     5641 agcaagcagc gccgcaggac gccgcagcgg tcgtcgcgat catgatcgcc gttgcatcga
     5701 acacgatcgt gaagtcatcg ctcgcactcg gcatcggcgg ccgccggctc ggcaagcgtg
     5761 cgttcttgat cggcggcctg atggtcgtcg gtgccgcgct cggtctgatt ccggtcgtgg
     5821 tctagctgac gaagctgatc tgccactcgc gcacatcacc gtcgacggtg atgtcgacga
     5881 catcaccaac gcggcgaccg agcactgctt tgccgatcgg cgaggccggc gtgacgaccg
     5941 agagtacgcc gtcgccgccg gggccgctca acgtcatgcc tgcaccgacg ggtgagagaa
     6001 agaatgtccg gccttcgccg gtatcctcgt cctcgatctc gacgatcgcg ccgacgccga
     6061 tcgtcgccat cttgcccggc ttgaagcgcg cgagcgcgtc gacatcggcc agcgcctgct
     6121 gggcgcggcg ttgctgggcg gcgccgagtg tctgcagttg atgcgcggcg cgtgcatcct
     6181 cgcgcttctc gtcgggcgtc gcgccttcgc gcgcctcttg tgccgcggca tcgcgcgccg
     6241 acagcgctga gcgggccgag ctctcgagtt gcttgaccag ctgggcgatc agctgttgct
     6301 tgtccatccc gaaagtgtcg ccgcctcatg cgcggtggac aaattttcag gactcgccgc
     6361 cggattgcgg caccgtcgac gcgatcgctg caccgccgag cagccagccg agaccgcaga
     6421 tgacggcgaa ccagctcgcg ccgaagatac cgccgagggt cgcaacgaca ccgccggcga
     6481 gcatcagctt tgcgaaccgg ctccgcttcg cgtcgtcgcg agagatcagc tcgacctgag
     6541 gccggctctc ggggcgccgt gactcgcgct cggcgagctt ggcgcgcaac tccgcgttct
     6601 cttgctcgag agcggcgatg cgcgcagcgg cgtcctcgtt gtctgccacg cgatcgagtg
     6661 tacgatgcgc gcatgttgaa ggtcggcgat ccgatgcccg atgtcacgtt ggttggcgag
     6721 accggtcccg tccagcttcg ttcgttcatc ggcaagcccg ccgtcgtcta cttctatccg
     6781 aaggacgaga cgtatggctg cacgaaggaa gcgtgcagct ttcgcgattc gtacgaggac
     6841 ttcgtcggtg caggcgccca ggtgatcggc gtgtcgcgcg acgacgccgc atcgcacgcg
     6901 agcttcaagc aacaccatcg gctgccgttc acgctgctca gcgatccggg cggccagctc
     6961 gcgaaagcgt ggggcgtgaa gggcgggctc ggcttgccgg gccgcgtgac gttcgtgttc
     7021 gacaaggacg gcgtgtgccg gcaccgcttc gattcgcaga ttcgcttcgg caagcacgtc
     7081 gacgaggccc ttgaagtcgt caagcaactc gccgccgctt agcgcatcgc cgcctgccgc
     7141 gtcgccgccg catggcgcgt cgctcgcgcg ggtgctcggc ttgccgggcg cggtgctgat
     7201 gggcctcggc tcgatcgtgg ggacggggat cttcgtcagc atcgggaccg ccgccggcgt
     7261 cgccggctcg gccgtgctcg tcgcggtcgg gctcgccgcg atcgtcgcga cgtgtaacgg
     7321 cctatcgagc gcgcagctcg cggccgcgca tccagtgagt ggcggcgtct acgagcaggc
     7381 ctatcgctac gcgacgccaa ccctcggccg catcgcgggc tttgcgttcg tgtccgcgaa
     7441 gaccgcatcg gcggcaaccg ccgcgcttgg ttgtgcgggc tacgcgctcg cgctggcgag
     7501 cagtggcgat cgcacggtgc agatcgtcgt cgctgcgatg gtggtggtcg tgctgaccat
     7561 cttcgtcgcc gggggcatgc agcgatcgag tcgcctgaac gccgtcgtcg tcgcgctcac
     7621 gctcgtctcg ttgctggtgt tcgtagtcgc cggtttgccg cacgtcgatg tcgcgagact
     7681 gaacccgacc ggctatgcgc cctcggcacg cgctcccggc gcagacgata tgcaacttgg
     7741 ctcgctggtc cgcgatatcg cgcatgcgac cgcgctgatg ttcgtcgcgt acaccggtta
     7801 tggccggatc gcgacactgg gcgaagaagt acgcgagccc gcgcgcacga tcccacgcgc
     7861 gatcatcgtc acgctcgtcg tcgcgctctt gctctatctc gccgtgacca cggtcgcaat
     7921 cggatcggtt ggcgccgacg gcctcgccgc cgcgactcgc gccggcagtg cgccgctcga
     7981 gacgatcgcg gcgggcttcg gcgaaccaat tcgctacatc gtcgccgccg gcgcgatcac
     8041 cgcgatgctc ggcgtgctgc tcaacttgct gctcggcatt tcgcgcgtcg tgctcgcgat
     8101 ggcacggcgc cgcgacctcc cgcacgggct tgccaccgtt cacggctcgt cgccacgccg
     8161 cgcggtgatc gtcacgggtg tcgcgatcgc cgcgctcgcg ctgatcggca gcgtgaagac
     8221 gacgtggtcg gtgagcgcgt tcttcgtgct cgtctactac gggatcgcga actactgcgc
     8281 gctacgccag ccagcgagcg aacgccggtt tacgcgcgcg gtgcccgtgc tcgggctcgt
     8341 atcgtgcctc gcactcgcag tgtggctcgt gatcgatgtg ctcagcccat gaccgacgtg
     8401 ctcagccctt gagcgcggac acgaccttct cgatcgcatc cttgctgtcg ccaaacagca
     8461 tcttcgtgcc cggcttgtag aacagcgggt tgtcgatgcc tgcgtagcct gccgcgcgcg
     8521 agcgcttgtt gacgacgacg agctttgctt tccacacctg gagcacgggc atgccggcaa
     8581 tcgggctcgt cggatcttcc tcggcggccg ggttgacgat gtcgttcgcg ccgatcacga
     8641 tcgcgacatc cgtctgcgga aagtcgtgat tgatctcgtc catctcgagc acgatgtcgt
     8701 acggcacgtt cgcttcggcg agcagcacgt tcatgtggcc gggcagacga ccggcgacgg
     8761 gatggatcgc gaagcggacg ttgacgccgt ccttgcggag gacgtccgtc atctcgcgca
     8821 ccgcgtgctg agcgcgcgcg gcggccatgc cgaagcccgg cacgatgatg acgttcttgc
     8881 tgccctttag cgtcgcggcg agcgtcggtg cgtcgatctc ctgtacctcg ccggcgggtt
     8941 gcgcaggacc cttcgccgcc gccttcggtg gcgcagcgcc aaaaccgccg aagatgacgt
     9001 tccagatcga acggttcatc gcacggcaca tgatgtagga gaggatcgcg ccgctcgaac
     9061 cgacgagcgc gccggtgatg atgagcaggt cgttgtggag catgaagccg gcggcggcag
     9121 cggtccatcc cgagtagctg ttgagcatcg agacgacgac tggcatgtcg gcaccgccga
     9181 tcgcggcgac gaggtgcatg ccgagcacac cggcgaggcc gctcgcgacg agcaacttcc
     9241 aggtctccga cttttcgccg tagagcgcgc cgagcgtgac gatgccgatc agcagggcga
     9301 ggttcgcgaa gtgacgtgcc ggcagcagca atggcttgcc cgacacggag ccccgcagct
     9361 tcaagaacgc gaggaccgag cccgtcgtcg tgatcgcgcc gatgatgacg tcgacgtaga
     9421 tctcgacctt ctcggccatc ggcaagaacg tgccgagatg gagctggagc gagaatccga
     9481 cgagcaccgc ggcgaggccg acgaagctgt ggagcaacgc gaccatctcc ggcatctgcg
     9541 tcatgccgac gcgcaacgcc atcgtcgcgc cgatcacgat cgcaatgacg atcgagccga
     9601 gcagcagcgg atggaagttc gcgccgcgga acgacagtgt cgcgccgatc gcgatcgcca
     9661 taccgacgat gccgtacacg ttgccgcgcc tcgcggtctc ttggctcgac aacccgcgca
     9721 acgcgaggat gaacagcacg gccgcgacga gatacgcgac cgtcgccagc gcgtaccact
     9781 gcggaatctt gttgtacatg tccacggcta tttcctgaac atcgcgagca tgcgccgcgt
     9841 aacgatgaaa ccgccggcga tattgatggt cgcgaacaac gtcgcgatca cggcaacgat
     9901 caccgagctc gtgagcttgc cactaccggc gagcaagcca ccgacgacga tgatgccgct
     9961 gatcgcgttc gtcacgctca tcagaggcgt atgcagcgcg ggcgtgacgt tccagacgac
    10021 ctggaagccg atgaacacgg ccatcacgaa cacggtgaag cgctggatca gctcggtggt
    10081 ctccgccgag cccatcgcat cgggcttcgc gaagcgcagc gacagccagg cgatcgcggc
    10141 caggatgccg atcaggctca tgatgatgcc gccggcacgc gttggcgggc tcgtcgctgc
    10201 gccgtgaccg tgcacacccg acggcttcgg cggatgcgac acggctggct tcgcttctgg
    10261 cttcggcggc tctggcttcg gcggcggcgc gaccggcgct tcggccttct tcatcggcgg
    10321 cgcgggccag cgcttctcgc cgttctcgag cacgagcgcc gggcgaaccg cgtcgttctc
    10381 gtgatcgatc ttgtacttcg cggcaccgcc catctcgtcg aggaagtgcc agaggttcat
    10441 gccgtacagg tcgctcgcga tgtgcgccat gcggctcggc agatcggtgt agccgatgat
    10501 ggtgacgccg ttctgatcga tcgcttcgcc cggcttcgtg tactcgcagt tgccgccttg
    10561 ttcggcggcg agatcgacga cgaccgagcc cggcttcatc atgtcgacca tgtctttgag
    10621 ccacagcttc ggcgccggct tgccgggaat cagcgccgtc gtgatcacga catcgacgtc
    10681 cttcgcctgc tcgcggaaca gcgcgtactc cgcgtcgatg aactccttgc tcatctcctt
    10741 cgcgtagccg ccctggcctt cgcccgcttc cttgatcttc acggtcaaga actcggcgcc
    10801 catcgacttc acttgctctt cggcggcggc gcgcacgtcg aacgcacgca cgatcgcgcc
    10861 gaggcccttc gcagcgccaa gtgcggcgag gccggcaacg cccgcgccga tcacgagcac
    10921 cttgcctggc ggcaccttgc cagcggcggt gaactgaccg gtgaagaagc tgccgaaccg
    10981 gttcgcagcc tcgatgaccg cgcgataacc ggagatgttc tgcatcgacg acagcgcgtc
    11041 catcttctgg gcgcggctga tgcgcggaac ctgatccatc gcgaggacgg tcagcttctt
    11101 cgcggcgagc ttggcgacga gcgcttcgct ctgcgccgga tagatgaacg agatcagggt
    11161 gccgccgtcg cgcagctgat cgacttcgtt cagctcgggc ggccgcacct tgagcacgat
    11221 gtcggcgcgc catgcggtga ggcgatcgac catcgtcgcg cctgccatct cgtacagcga
    11281 gtccgggtaa ctcgccgttg cacccgcacc acgctcgacc tcgacctgcc agccggcctt
    11341 cacccacttg ccaacgacat tcggcgacgc ggcgacacgg cgttcgagtg gccgcgtctc
    11401 cttaacaatg cctaacttca tggccaggcc ggaccatgcc aaaccaatcg caatcgcgaa
    11461 agggctgcgt tacacaaagg tcgcgtgatc gaagtagccg gcctgaccgt gcaccaggac
    11521 gtggtcgagc acccgctgct tggcgacgct cacatcatcg agcacgcggg tgagccgatc
    11581 accgcgatgt cggcgatcga ctgggatcga ccgacgcgga ttccgacggt cgctgaaccg
    11641 cgtgcgctcc cgcgtggcac gggaagcatg ctcatcaacg aaatcgcatc acgcgcgcaa
    11701 cgagctggcg tccaggcgct gcgctacgcc gggccctacc cgacacccgc actgttcaac
    11761 acgctgaagc ggtcctttcg aacgaccgca acggagtcga tcttctcgtc ggacgtcctc
    11821 ggccgcgcga tgcgcctcgc acgtgacgaa gtgccggttg atttcgtacc ggctccgttc
    11881 acgcgtcagc cgatgcccta cggctcgatc gacgtgcgcg acggaatcga gcgcgcggtg
    11941 atcaacggca tcttgttcga tcaggaaccg acggtcggct ccctcgcacg cctcgtcggg
    12001 atcgaagcgc cgatcgggtc gacggcgagc ggtgctgccg cggtgctcgc gttcgacacc
    12061 gtcgtgtgga cacatgttgc cgagctcgca accgacggca gcctcgtcgg cgacgtgcgc
    12121 ccaatcccgg cgctcgacag caacgtgatc ggcaagcagt ttcccgccga gctcaagcag
    12181 attttctcgg aattcgtcgc gagctccgac gccatccccg gcccgctgtg ccgcgacacc
    12241 gagcgtgtgc tgagcgaacg gctgatcacg tggcgcgacc tcggctggcg tgctgctgcg
    12301 cgcgaaggcg atggcttcgc actgcacgtc gggtggtgga cgcatctggt cccgcgcgga
    12361 ttcgagacgc tcgtccgcgc gctcgccgac gccattgcac cggtcgcggt gcagctcatc
    12421 ctcgacgagg tcacgcgctg atttcggaca gcgcaacaac ggcccgctac tcgctgctcg
    12481 ggtggacgcc gacgatgatc ttgatgatcg cgccatcgtc gccgccccac gacggtccgg
    12541 cgccgacgtt cagatcgagc cagcgtccag cgtagtcgac ggcagcaagg agcgttccta
    12601 cgtgctcgct gccgagatca tcgatcggcc cgaacgcgcc gtacgcttca acgccgaggc
    12661 tccagcgcgg tgacaggcgc ccggcgagct tcacagcggg ctcgagctgg ggatgaccgg
    12721 caagctcgcc acgcagatcg atcgcgagga tcgggttgat cgatgcgtag aagtccgtcg
    12781 tcgtcagatc ggcgatcggc cggacctcac tgccccagac gtcgggctcg aactgttcag
    12841 gcaccgctga aacctcggtg ttgaccgcga gaccgatgcg gtcgtgccac aggcgtcgcg
    12901 gcattctcgc tttcacgcgc aatttgccgc cgccgaacgc gacgtcgccg tcggtgagtg
    12961 ctgtttgcag atagccgccg acctcgagcc attcgagcac gccgtagtgc ggctcgaacg
    13021 tcaggtgcga tgcatcggca gcgtcgcgaa cgaagtgatg gttcgcgtgg acctcgagcc
    13081 ccgcttcgcc gcgtgcggcc gtgtcactcg cgtagacctg gatctcgaat gcgtcttgcg
    13141 cgcgagcagg tgtcaccagc gctgcggccg tcacgaagca gagccagcgc gcgtacacac
    13201 tgcgttctag caggccggct ccccgagcat atcgcggctg ctacgtacgc cgtgtcggtg
    13261 ccggatcgac gcgctactcg tcgtgggtgc cgttcgcgcc gttcgcgccg ttcgcgccgt
    13321 tcgcgccgtt tgcgccgttc gccccgttcg ccccgttcgg tgcgtgcccg ttcgccgcga
    13381 tcatcatgcc gccggtggct gcaaactgat cgaagtcgat gtccatcgtc cacttgccgc
    13441 gcttcgtcgg ctcgtcgagc gcgcgggcga gcatctgtag atactcgaga cgcggaatct
    13501 cgtacgcgcc gaaccgctcg aggtgctcgg tgtggacctg gcaatcgatc aatccgatgt
    13561 gccacgaatc gagctggcgc acgcttgcgg cgaacgcgat cttcgacgca tcgggcgcct
    13621 tcgcgaacat cgactcgccg aagaacgccg cgccgagcga aacgccgtac aggccaccga
    13681 cgagctcacc acccttccac gcctcgaacg agtgcgcgaa gccgagctcg tgcagcttct
    13741 tgtaggcgtc gaccatttcc ggaatcagcc acgtgccggt ctggccgggt cgcggcacat
    13801 tcgcgcagcc gcgcagcacc tcttcaaagg ccttgtccgt cgtcagctcg tacggccgcc
    13861 gcttgatcgc cttgcgcagg ctgcgctgca cgatcagatc gcgcgtcgtc atcaccatgc
    13921 gtggatccgg cgaatgccac aggatcggca ggttctccgc gtaccagggg aagatgccct
    13981 gcgtgtagcc gagcaacagg cgttccggac gcaggtcacc accgagcgca aggaggccgt
    14041 cctcggcaag gtggaccggc ggaaacacga gtcgatcgtc gagccggaaa accggcatag
    14101 gccgagtgta gcggatagag tcgggccatg aagtggttcg tgatcgcggt ggctattctg
    14161 gccggttgcg ccccggtcgc accgcgattc ccgaccgacg tccagaccgc cctgtcggcg
    14221 gacatgcgcc gcagcgagat gcgcaggctc gagaccgagc gcttcatcat ctactaccca
    14281 ggctggaatc gtttcggagt cgaacgcctt ctcgagcgtg ccgaccgctg cctccagacg
    14341 ctgcgcgagc acgcgctcat caagacgacg aagaagatcg tcatgctgat gcccgacgtt
    14401 ccgttcaaca acgcgttcgt catgcccgac gcactcggct acgagcagat ctcggtcatc
    14461 ccgacccact tcacgctcga cttcgcgact gcgttcggcc tgccgcccga tccgggcgcg
    14521 gtcgcgtgcc acgagctcac gcactacgtc cacttcgagc agacggcggg attctgggga
    14581 acgttcaacg agtggttcgg cccgatgtac acgccgcagc tcggctacga cccgtggttc
    14641 gacgaaggca tcgcgaccca ctacgaagcg cgcctctcgc ccggcctcgg ccgcccggtg
    14701 tggccgctgt tcaccggcat gtttgccgct gcctacgcgg gccgcgacat caatagcggc
    14761 gagctctcat cgttcgcgcg cgaagcgtcc gttggccatc actatctcgt cggttcgatg
    14821 ttcgtgcgct ttctcgtcga gaaatacggc gagcgcccgc tgtggacgac gatcgagaac
    14881 caagcccacg cgttcaccgg ctggttcttc accggcacgt tcaagcacgg cttcgcggtc
    14941 tcgttcagcg atctgttcgc tcaattcggc cgctgggtcc acgagacatt cccggtccgc
    15001 gtgcgcccgc ccacccaacg caagctcgcg acgatcggca acgacgcgcg atacgctcgc
    15061 ggtcgcgacg gcaccgaagc atgggtcgcc gaggacgtcg actcgccgcc gcgcctgacc
    15121 gtgcgcgacg cacacggcgc gactctgctc gacaccgcga tcaccgaggt ggtgccgccg
    15181 cgcacggtcg cgaacgctga tccattgctc gtgtcgggcc tctcgatcac cgccgacggc
    15241 aacgaggtct ggctcaccgc gatcgatctc ggctcgacct atcaggtcac gcgccttttg
    15301 cgctggcggc gcggcgagcg cggcctcacc gaagtcgcgc atgacctcgg ccccggcgcg
    15361 acgatcgatc cgacgggcag cacgtactac tactgccagg tcagtggcag tcactggagc
    15421 cttctcgcct acgacgtgaa gcgcggcgtc acggaccgga tcgtcatgga catgaacagc
    15481 gagacgggcg tgtatgtgct cggtgcgcag gtatcgcccg acggcaagca actcgccgcg
    15541 aacgtgtgga acggctccgc cttcgtgatc tggatcgtcg acgcgcagca cggcgtgttc
    15601 gcgcgcgaga tcaagggcaa cggcacgccg gtgtacgacg cgagcttcac cgccgacggc
    15661 cgtgtcatgt acctcggcgt cgtcgatggc cgcttccagg cattcatcga aggcacacag
    15721 gtcaccgacg caccatacgc cgtgctcgca gcgcgcgacg ccggcggtac gatccgcttc
    15781 ctcgatcgcg agcactggga atgggagctc gccgaagttg cgacaccacc taaacccgct
    15841 ccgactccgc cgactccgcc gactccgccg actccgccga ctccgccgac tccgccgact
    15901 ccgccggaag gaactccttc cccggacgct gcgccgacaa cggatgcacc ccaaccagca
    15961 gccggacaac caccggcagc acagccagca accggacaac cagtaaccgg acaaccgcca
    16021 accggacaac cagtaaccgg acaaccgcca accggacaac cgccaaccgg acaaccacca
    16081 accggacaac ccgcaaccgg acaaccggca accggacaac tggcaac
//
LOCUS       DASUFX010000639        10163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1908178, whole genome shotgun sequence.
ACCESSION   DASUFX010000639 DASUFX010000000
VERSION     DASUFX010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908178"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2255)
                     /locus_tag="VFV99_25790"
     CDS             complement(<1..2255)
                     /locus_tag="VFV99_25790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX5062822.1"
                     /translation="MTKRSATRSSPKKKSAEPAPKAAKAKGARRASKAEEPEEVVETP
                     APKKAEKAVKAAKAEKPEKPEKAAKAAGTRAKKVAEPPPPPPRPVYDLNGPVPAEGEG
                     LPKKVGALLGIGQKALGIKSFRPGQAEAFDHLLGGEDLLAVMPTGSGKSLLYQLTSLV
                     VPGVTVVVSPLIALIKDQLDKMTAKGVTAAKIDSTLTVKQRREVDALVRSPGGKLLLT
                     TPERMADPEFRVFLREASEGVGVSRFVVDEAHCVSQWGHDFRPAYLSLRKALEDVGRP
                     PVLATTATAPPHVREDILFQLGMEKATIVTTTFDRPNLHYEVIVFHDEDEKMKTLVTL
                     LKKLPRPGIVYCATVKKVEELYEALTRWGVPTGKYHGRMNKGERDESQQKFMESSDLI
                     MVATNAFGLGVDKANVRNVLHYHVPGSLEAYAQEAGRGGRDGKPSRCVLLFSPDDVAI
                     QEYFLSGTYPTKRQVRQVYETIEHFAAATGKLAEESVPNVANIALGSSVGQQRTRTVL
                     SLLKDEGFIVERDGGTFHLAQPTREKEELMERAKQYEARRIADRQRLDALLTYVKAPG
                     CRNQVILEYLGEPQPPKCGRCDNCLRSREAALAASTEATRFGASITRQLDDDGVELPK
                     PRREIKARVVRIDQQQPVVTAPNGDTTTVIEPAKPAAPSTVLRRAKPSTEAPAPAASR
                     PGSSNGHPAAKAPAPAAVPSAAPTPITAKGSEPLVAAGAAVVRKKPVPAPEPKELEEE
                     DEDDLEDDEEEL"
     gene            2415..4031
                     /locus_tag="VFV99_25795"
     CDS             2415..4031
                     /locus_tag="VFV99_25795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5062823.1"
                     /translation="MGSDRPVREAENRLERFNADREKLLAEVERRVVARRSAEAKAGG
                     DASLEYVLNDVAYSEIKRLEKSKNGAAERWRDLSRRLLTMSEEEKQAELRSLVRYYGH
                     DVVGNFDPRVYKFATGVGPSLLGVLFNPLGSLKDGMAALRNLDSRIHADGELEVAHTC
                     AERGTIVVTPTHSSNMDSPAIGFGLLKAGLPPATYGAGKNLFTNPFISFFMRNLGAYR
                     VDRRLRFELYKDVLKEYSTVLLEHGYHSLFFPGGTRCRSNIIERHLKLGLLGTTVTAY
                     KNLVREGRANKRLYIVPATINYRLVLEAETLIDDYLAETGKNRYIITDDEFSRVGRMF
                     EFFRKIIAHEGSVIVRFGRPFDPFGNDVTDDGESIDRAGRIVDPASFVRGAEGEVSDD
                     DQRDAEYTRALGRRLAQVFPKLSVFHSTSLLARVLYDAVSRAAGTRDIYRLIRATPQQ
                     LAVDLPNAIDGVERLRRRLREHPEWGAEHPHFTEQSSGDKLEDAVRGLSTYHTRPIVE
                     RGGATLHVKDIKLLYYYQNRLAHIPVEASS"
     gene            4028..5014
                     /locus_tag="VFV99_25800"
     CDS             4028..5014
                     /locus_tag="VFV99_25800"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5062824.1"
                     /translation="MKRADTVGVIGAGSFGTALASVLARAGRRVVLWSRDPSVVNAIN
                     KTRTCPRLPNAPLPQPLEATVDPRRLAAEARFLVMAVSSTDVVERAKEVGSFLDGSHI
                     VVHAIGALAAPTNERVSEVMEQGLPTLKIGVIAGPALPADLAAGEFSSVVVASRFDEV
                     VSEARRLLNAPPTLRVYSSHDLPGVELSSALAGAYTVALGLCDGLDIGAGARAVLITR
                     VVAEAGRLIHAAGAEAKTFAGLAGLGNMLVRGGKRIGSEDYQLGRRLAEGVISADSER
                     TEGARAALAGASLADRLRVRMPVLSGVAAVLAGRAEPRDAAKLIGDTVAIEE"
     gene            5015..6262
                     /locus_tag="VFV99_25805"
     CDS             5015..6262
                     /locus_tag="VFV99_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062825.1"
                     /translation="MKRLALAALAVGSASCHTHTTPFNVKAPDAGVSIAIYDKAGGDG
                     FAVVDDRRWLDVSGKTIMLANVDPGAELASLVIEPADRALSVGQCVRERMPDIPPKDP
                     LEAYANEQRDRRALELRRQMANVRALRPPVREPTQPVQEDHFVPVVKCDVSAKPGRYL
                     VRVLYVTKAIDYRAQHDIDVTDEKTARVTSRFAIVTPVWQTHAELVLYDGVPGGDRSP
                     EEVARGQVTLDGSTSVLSVPTHDVPAQLQRIYEGAVVTSTDTTDITWGSGSAQAIWVW
                     LDLGKMRLAPGPVRVHLDLAGEGVRDLDVAQSMRRQDDTPDAPLRLPLWVDESLRGSR
                     TRLPEYNDSSMITERYMFSVANTGETERTVIVEEPMRTATRRKLERAWPKKPTADHNV
                     LRTKLDVKPGRIERTGYTMTYDF"
     gene            6266..6715
                     /locus_tag="VFV99_25810"
     CDS             6266..6715
                     /locus_tag="VFV99_25810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062826.1"
                     /translation="MPKPKKKTAPAKTKTLPGKKKAVVAKQIAIGVPAKKPTGKTGQQ
                     RALSREDIHVALGEQLTATRGNETKERAAIASAINAFMQLAIASGAAADTSAYAASCP
                     EFFWEGDGANPPVYARVPDATHDEIGRWFQLIVEGVRAYDARVGATA"
     gene            complement(6705..7217)
                     /locus_tag="VFV99_25815"
     CDS             complement(6705..7217)
                     /locus_tag="VFV99_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5062827.1"
                     /translation="MVRECVALSVAALVPGCSLILNFDESAPVDASIDAPYTQAECDY
                     KEPNNSLADAVMIDPSTDTGPAAICKPMTSGSPEDEDFYKFTVVNASVTVSITFMNRT
                     MGDLDIKLWDAANMTNPVAQSRGFGDGETITCPGASPSCPTLTPGDYILQVFPAVPGS
                     VNSYTFMIMP"
     gene            7328..8317
                     /locus_tag="VFV99_25820"
     CDS             7328..8317
                     /locus_tag="VFV99_25820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5062828.1"
                     /translation="MYLQDLIFTLSKYWADRGCVIEQPVDVEVGAGTFHPATFLRVLG
                     PEPWNAAFVQFCRRPSDGRYGENPNRAGAYYQFQVGLKPTPLNSQDLYLDSLRAIGLD
                     PSTHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVTQYTYFQQAGGIDLLPSTTELTYG
                     IERIAMYLQGVDNMYDLKWDKHVTYGQIRHPWEVEYSKYHFEELKPEIAFRNFDSYEG
                     ECQRLLERGLVLPAYEFCMKSSHAFNTLDARGAISVTERARFIGRVRAMARACAHEYV
                     KSRARLGFPLLPKQLGAQAKEAFDGVGEAGVNAGALAAARVVVPHAEGLPHAG"
     gene            8307..>10163
                     /gene="glyS"
                     /locus_tag="VFV99_25825"
     CDS             8307..>10163
                     /gene="glyS"
                     /locus_tag="VFV99_25825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5062829.1"
                     /translation="MPADLLFEIGCEEIPAKMLAKAIADLPAAVEQKLTAARLEWSAV
                     RAVGTPRRLAVIVKGLSDRQPDLNEEVVGPPVNAAFAPDGTLTKAGQGFAAKNGVAPS
                     IAASALVKKEVAGKKGLYVVAQRHVVGNETRALLPELLRELAAGIPWPKSMRWGWSET
                     AFVRPVQWLVALFGGEVVPLTWAGQTSGRKSRGHRFLSPGWIEIASPDKYVDALREAH
                     VVVDPDARRDLVRAELSRIEKDTKLRVRPDEALINEVINLGEYPVGVSGTFDPSFLEV
                     PEEIIVTTMRNNQRYFAMEDGSGKLAPRFVTMMATIVKDPAVVARGNEYVIASRLADA
                     KFFFAEDRKKTFDDWNAKLDGVVFQAKLGDGAKTIGNKVARIAGIVEALGGSATAKQA
                     ARYAKSDLASLAVGEFPEVQGIMGRHYAKLAGLGDGVATVIDQHWWPKGQGAALPTTD
                     DAALVALADRMDTIVGCFAVGLEPSGSADPFGLRRAAIGIWQILLDRGWTQHYARLRQ
                     AAETQLVAQGVKLKDGKPLDEFFRTRLRGIFVEQDIPPQDADAALAQDYRDPIDARAR
                     ALACAKISKEAREVFKRVANILDDARGKKLTIGASPDPSLFVAKVETDLHAAI"
BASE COUNT         1712 a         3342 c         3364 g         1745 t
ORIGIN      
        1 agctcttcct cgtcgtcctc gagatcgtcc tcgtcctctt cctcgagctc tttgggctcg
       61 ggcgcgggga ccggcttctt gcgcacgacc gcggcacccg cggcgacgag gggctcggag
      121 cccttcgccg tgatcggcgt cggagccgcc gatggcactg ccgccggggc cggagccttc
      181 gcggctgggt gtccgttgct cgacccgggc cgcgacgctg ctggtgccgg cgcttccgtg
      241 ctcggctttg cgcgtcggag caccgtcgac ggtgccgcag gcttcgccgg ctcgatcacc
      301 gtggtcgtat cgccgttcgg cgcggtcacc acgggttgct gctgatcgat gcgcacgacg
      361 cgcgctttga tctcgcggcg tggcttcggt agctcgacac catcgtcgtc gagctggcgg
      421 gtaatcgatg cgccaaaccg cgtcgcttcc gtcgaggcag cgagtgctgc ttcgcgcgaa
      481 cgcagacagt tgtcgcaacg cccgcacttc ggcggctgcg gctcgccgag atactccagg
      541 atcacctggt tgcggcagcc cggcgccttc acgtacgtaa gcagtgcatc gaggcgttgg
      601 cgatcagcga tgcgccgcgc ttcatactgc ttcgcgcgct ccatcaactc ttccttctcg
      661 cgcgttggct gggcgagatg gaacgtgccg ccgtcgcgct cgacgatgaa gccttcgtct
      721 tttagcagcg atagcacggt gcgcgtgcgc tgctggccga cgctcgagcc gagcgcgatg
      781 ttcgcgacgt tcggcactga ctcctcggcg agcttgccgg tcgccgccgc gaagtgctcg
      841 atggtctcgt agacctgacg cacctggcgc ttcgtcggat acgtccccga caagaagtac
      901 tcttgaatcg cgacgtcgtc gggcgagaac aacagcacgc agcgtgacgg cttgccatcg
      961 cggccgccgc gtcccgcttc ctgtgcgtac gcttcgagcg atccgggcac gtgatagtgc
     1021 agaacgttgc ggacgttcgc cttgtcgacg ccgagaccga acgcgttggt cgcgaccatg
     1081 atcaggtcgc tcgactccat gaacttctgc tgcgactcgt cgcgctcgcc cttgttcatg
     1141 cggccgtggt acttgcccgt cggcacgccc cagcgcgtga gcgcttcgta cagctcttcg
     1201 accttcttga cggtcgcgca gtacacgatg cccggtcgcg gcagcttctt cagcagcgtg
     1261 accagcgtct tcatcttctc gtcttcgtcg tggaagacga tcacttcgta atggaggttc
     1321 ggacgatcga acgtcgtcgt gacgatcgtc gccttctcca tgccgagctg gaacaggatg
     1381 tcctcgcgca cgtgcggcgg cgcggtggcc gtcgtcgcaa gcacgggcgg acgaccgacg
     1441 tcctcgagcg ccttgcgtag cgagagatac gcggggcgga aatcgtgacc ccactgggag
     1501 acgcagtgtg cttcgtcgac gacgaagcgc gacacgccga caccctccga ggcctcgcgc
     1561 aagaacacgc ggaactcggg atcggccatg cgctcgggcg tcgtgagcag cagcttgccg
     1621 cccggcgagc gcacgagcgc gtcgacttcg cggcgctgtt tcaccgtcag cgtcgaatcg
     1681 atcttcgctg cggtcacgcc cttcgcggtc atcttgtcga gctgatcttt gatgagcgcg
     1741 atcagcggac tgaccacgac ggtcacgccc ggcacgacga gtgacgtgag ctgatagagc
     1801 agcgacttgc cgctaccggt cggcatcacc gcgagcaggt cttcgccgcc gagcaggtga
     1861 tcgaacgcct cggcttggcc gggccggaac gacttgatgc cgagcgcctt ctggccgatc
     1921 ccgagcagcg cgccgacttt cttcggcaga ccttcgccct cggcgggcac gggtccgttc
     1981 aagtcgtaga ccgggcgcgg aggaggtgga ggtggctccg ctaccttctt agcccgagtg
     2041 ccggcggcct ttgccgcttt ctccggcttc tccggcttct ccgctttcgc ggccttcacc
     2101 gccttctccg ccttcttcgg cgcaggcgtt tcgacaactt cttcaggctc ttccgctttc
     2161 gacgcacgac gagcgccctt ggccttcgcc gccttgggcg caggctctgc agacttcttt
     2221 ttggggctac tccgtgtagc cgaccgcttg gtcaaggacc acgactccta tccggctccg
     2281 ccggccggta tcagctggct gcagcagcct ctagagagag gccgccgcgt cacttaatga
     2341 agaacatacc atgagggcct aggcaagggg catgaggcga tcttcgggat ttcttggtgt
     2401 gttacaggat cctcatgggc agcgatcggc cggtccggga agccgaaaat cggctggaga
     2461 ggttcaacgc tgaccgcgag aagttgctgg ccgaggtcga gcgcagggtc gtcgcgaggc
     2521 gttcggccga ggccaaggcg ggcggcgatg cctcactgga atacgtgctc aacgatgtcg
     2581 cgtactccga gatcaagcga ctcgaaaaga gcaagaacgg cgcggccgag cgttggcgcg
     2641 acctatcgcg ccgactgctc acgatgagcg aagaggaaaa gcaggcagag ctacgcagct
     2701 tggtccgcta ctacggccac gacgtggtcg gcaattttga tccgcgcgtg tacaaatttg
     2761 ccactggggt cggaccatcg ctgctcggcg tcttgttcaa cccgctcggc agcctcaaag
     2821 acggtatggc cgcgctccgc aacctcgaca gtcgcattca cgccgatggc gagctcgagg
     2881 tcgcgcacac ctgcgccgag cgcggcacga tcgtggtgac gccgacgcac tcctcgaaca
     2941 tggactcgcc cgcgatcggc tttggcctac tcaaggcggg cctaccgccg gcgacgtacg
     3001 gcgccggcaa gaacctcttc acgaacccgt tcatctcgtt cttcatgcgc aacctcggcg
     3061 cgtatcgcgt cgatcgccgg ctgcgcttcg agctctacaa ggacgtgctg aaggagtact
     3121 cgaccgtgct tctcgagcac ggctaccact cgctgttctt tcccggcggc acgcgctgcc
     3181 gctcgaacat catcgagagg catctcaagc tcggcctgct cggcacgacg gtcaccgcgt
     3241 acaagaacct cgttcgcgaa ggcagggcga acaagcgtct ctacatcgtg ccggcgacga
     3301 tcaactaccg actcgtgctc gaagccgaga cgctgatcga cgactacctc gccgagaccg
     3361 gcaagaatcg ctacatcatc accgacgacg agttctcgcg cgtcggtcgc atgttcgagt
     3421 tcttccgcaa gatcatcgcg cacgagggca gcgtgatcgt gcggttcggt cggccgttcg
     3481 atccgttcgg taacgacgtc accgacgacg gcgagtcgat cgatcgcgcg ggccgcatcg
     3541 tcgatccggc gagcttcgtg cgcggcgccg agggcgaagt gtccgatgac gatcagcgcg
     3601 acgccgagta cacgcgtgcg ctcggccgcc ggctcgcgca ggtttttccg aagctcagcg
     3661 tgttccactc gacgagcttg ctcgcacgcg tgctgtacga cgcggtctcg cgcgctgcgg
     3721 gcacacgcga catctatcgc ctcattcgtg cgacgccgca gcagctcgcc gtcgatctac
     3781 cgaatgcgat cgatggcgtc gagcggttgc gccggcggct gcgcgagcac cccgagtggg
     3841 gcgccgagca tccgcacttc accgagcagt cgtccggcga caagctcgag gacgcggtgc
     3901 gcggcctgtc gacgtatcac acgcggccga tcgtggagcg cggcggcgcc acgctccacg
     3961 tcaaagacat caagctgctc tactactatc agaaccgcct cgcccacatt cctgtcgagg
     4021 cctcgtcgtg aagcgcgccg ataccgtcgg cgtcatcggt gcgggctcgt tcggcaccgc
     4081 gctggcgtcg gtactcgcgc gcgccggtcg ccgcgtcgtg ctgtggtcgc gtgacccgag
     4141 cgttgtcaac gcgatcaaca agacgcgcac gtgtccgcgg ctgccgaatg caccgctgcc
     4201 gcagccgctc gaagccaccg tcgatccgcg gcggctcgcg gccgaggcgc gcttcctcgt
     4261 catggcggtg tcgtcgacgg atgtcgtcga gcgcgcgaag gaagtcggga gctttctcga
     4321 cggcagtcac atcgtcgtcc acgcgatcgg tgcgctcgct gcgcccacca acgagcgcgt
     4381 gtccgaggtg atggagcagg gcctgccgac gctgaagatc ggggtgatcg ccggcccggc
     4441 gctgccggcc gatctggcgg ccggcgagtt ttcgtcggtc gtcgtcgcgt cgcgcttcga
     4501 tgaggtcgtg agcgaggcgc gccgtctact caacgcacca ccgacgctgc gggtctacag
     4561 ctcgcatgat cttcccggcg tcgagctgtc gtcggcgctc gcgggtgcgt acacggtcgc
     4621 gcttggcctc tgcgatggcc tcgacatcgg cgcgggggcg cgcgcggtcc tgatcacgcg
     4681 cgtcgtcgcc gaagcagggc ggctcatcca tgcggcgggc gccgaggcca agacgtttgc
     4741 ggggctcgcg ggcctcggca acatgctcgt gcgcggcggc aagcgtatcg gctcggagga
     4801 ctaccagctc ggccgcaggc ttgccgaggg cgtgatcagc gcggacagcg aacgtaccga
     4861 aggtgcgcgc gccgcactcg cgggcgcatc gctcgcggat cgcttacgtg tgcggatgcc
     4921 ggtactgtcc ggcgtggccg cggtacttgc cggacgtgca gagccgcgag atgcggctaa
     4981 gctgatcggt gacaccgtgg ctatcgagga ataggtgaag cgtctcgcgc tggccgcact
     5041 cgctgtcggc tcggcgtcgt gccatacgca cacgacaccg ttcaatgtga aagcgcccga
     5101 cgccggcgtg tcgatcgcga tctacgacaa ggcgggcggt gacggcttcg ctgtcgtcga
     5161 tgatcggcgc tggctcgatg tctccggcaa gacgatcatg ctcgcgaacg tcgatccggg
     5221 cgccgagctc gcatcgctcg tgatcgagcc cgcggaccgt gcgctgagcg ttggccaatg
     5281 cgtgcgcgag cgcatgcccg acattccgcc gaaggatccg ctcgaggcgt acgcgaacga
     5341 gcaacgcgat cgacgtgcac tcgagctgcg ccgtcagatg gcgaacgtgc gcgcgctcag
     5401 gccgcccgtc cgcgagccca cgcaaccggt ccaagaggat cacttcgttc cggtcgtgaa
     5461 gtgcgacgtc agtgcaaagc cgggccgcta tctcgttcgt gttctctacg tcacgaaggc
     5521 gatcgactat cgcgcgcagc acgacatcga cgttaccgac gagaaaaccg cgcgcgtcac
     5581 gtcgcgcttc gcgatcgtca cgccggtctg gcagacccac gccgagctcg tgctctacga
     5641 tggcgtgccc ggtggcgatc gatcgccgga agaagtcgcg cgcggccagg tcacgctcga
     5701 cggctcgacg tccgtgttgt ccgtgccgac gcacgacgtg ccggcgcagc tgcagcgtat
     5761 ctacgaaggt gcggtcgtca cgtcgacaga cacgacggac atcacgtggg gatcgggatc
     5821 ggctcaggcg atctgggtgt ggctcgatct cggcaagatg aggctcgcac ctggtcccgt
     5881 acgcgtgcac ctcgatctcg cgggtgaagg cgtgcgcgat ctcgacgtcg ctcagagcat
     5941 gcgcagacaa gacgacactc ccgacgcacc gctccgatta ccactgtggg tggatgaatc
     6001 gttacgcggc tcacgcacgc gtcttcccga gtacaacgac tcatcgatga tcaccgagcg
     6061 ttatatgttt agcgtcgcca acactggcga gaccgaacgt accgttatcg tcgaggaacc
     6121 gatgcgtacg gcgacgcggc gcaagctcga acgcgcgtgg ccgaaaaagc cgacagcaga
     6181 ccacaacgtg ctgcgcacga agctcgacgt gaaaccggga aggatcgaac gcacgggcta
     6241 cacgatgacg tacgatttct aggcaatgcc gaagccaaaa aagaaaacgg ctcctgcaaa
     6301 gacgaagaca ctccctggta aaaagaaggc cgtggtcgcg aagcaaatag cgatcggggt
     6361 gcccgcgaag aagccaacgg gcaagaccgg tcagcaacgt gcgctcagcc gtgaagacat
     6421 tcacgtcgca ctaggcgagc agctgacggc cacgcgcggt aacgagacga aagagcgcgc
     6481 agcgatcgca tcggcgatca acgcattcat gcagctcgcg atcgcaagcg gcgcggcagc
     6541 agacacgtcg gcctacgcgg cgtcgtgtcc cgagttcttc tgggaaggcg acggcgcgaa
     6601 cccgccggtc tatgcgcgcg tgcccgatgc aacgcacgac gagatcggtc gttggttcca
     6661 gctcatcgtc gaaggcgtcc gcgcgtatga cgcgcgcgtc ggcgctacgg catgatcatg
     6721 aacgtgtagg agttcaccga gccgggcacg gccgggaaca cctgcaggat gtagtcgccc
     6781 ggcgtgagcg tcgggcatga cggcgacgcg ccggggcacg tgatcgtctc gccgtcgccg
     6841 aatccgcgcg actgcgcgac cgggttcgtc atgttcgccg cgtcccacag cttgatgtcg
     6901 agatccccca tcgtgcgatt catgaacgtg atgctgaccg ttacactcgc gttcacgacg
     6961 gtgaacttgt agaagtcctc gtcttcggga ctgccgctcg tcatcggttt gcagatcgca
     7021 gcgggacctg tgtctgtcga cggatcgatc atcaccgcgt ccgcgagcga gttgttcggc
     7081 tctttgtagt cacactcggc ttgcgtgtac ggcgcatcga tcgacgcgtc gaccggcgct
     7141 gattcgtcga aattaagaat gagcgagcag ccgggcacga gggccgcgac ggacagagct
     7201 acgcactccc gcaccatgcg gcgaacctat aggttcgcgg gcgagaacgt caagtcatct
     7261 gggggactta gctcgcgacc gtcgcttgac catcggcact gccaaggcta gggtccaacc
     7321 gcctcgcatg tacctgcaag acctcatctt cacgctcagc aaatactggg cagatcgcgg
     7381 ttgtgtcatc gaacaaccgg tcgacgtcga ggtcggcgcc ggcaccttcc atcctgcgac
     7441 attcctgcgc gtgctcggac ccgagccgtg gaacgcggcg ttcgtgcagt tctgccgccg
     7501 gccgtccgat ggacgctacg gcgagaaccc gaaccgcgcc ggcgcgtatt accagttcca
     7561 ggtcggtctc aagccgacgc cgctcaactc gcaggatctc tacctcgact cgctgcgcgc
     7621 gatcgggctc gatccgtcga cgcacgacat tcgcttcgtc gaggacgact gggaatcgcc
     7681 gacgctcggc gcgtggggcc ttggctggga agtctggtgt gacggcatgg aggtaacgca
     7741 gtacacgtac ttccagcaag cgggcggcat cgacctgttg ccgtcgacga cggagctgac
     7801 ctacggcatc gagcgcatcg cgatgtacct gcagggcgtc gacaacatgt acgacctcaa
     7861 gtgggacaag cacgtgacgt acggccagat ccgccatccg tgggaggtcg agtactcgaa
     7921 gtaccacttc gaggagctca agcccgagat cgcgtttcgc aacttcgact cgtacgaggg
     7981 cgagtgccag cgcctgctcg agcgcggtct cgtcttgccc gcctacgagt tctgtatgaa
     8041 gagctcgcac gcattcaaca cgctcgacgc gcgcggcgcg atcagcgtca ccgagcgtgc
     8101 gcggttcatc ggccgcgttc gcgcgatggc gcgcgcatgc gcgcacgaat acgtgaagag
     8161 tcgagcacgg ctcggcttcc cgctgttgcc gaagcagctc ggcgcgcagg cgaaggaagc
     8221 gttcgatggc gtcggcgagg caggcgtcaa cgcgggcgcg ctcgctgcgg cgcgcgtcgt
     8281 tgtcccgcac gcggaggggc tgccccatgc cggctgattt gctgttcgag atcggctgcg
     8341 aagagattcc ggccaagatg ctcgcgaagg cgatcgccga tctgcccgcg gccgtcgagc
     8401 agaagctcac cgctgcgcgc ctcgagtgga gcgcggtgcg cgcggtcggc acgccgcgcc
     8461 ggctcgccgt gatcgtgaag ggcctctcgg atcgccagcc cgacctcaac gaggaggtcg
     8521 tcggtccgcc ggtgaacgcc gcgttcgcgc ccgacggcac gctgacgaaa gcgggccagg
     8581 ggttcgcggc gaagaacggc gttgcgccgt cgatcgccgc ttcggcactc gtgaagaagg
     8641 aagtcgcggg taagaagggg ttgtacgtcg tcgcgcagcg gcacgtcgtc ggtaacgaga
     8701 cgcgcgcgct gttgcccgag ctgctgcgcg agctcgccgc gggcattccg tggccgaagt
     8761 cgatgcgctg gggttggagc gagaccgcgt tcgtgcgccc ggtgcagtgg ctcgtcgcac
     8821 tgttcggcgg cgaggtcgtg ccgctcacgt gggcgggcca gacgtccggc cgcaagagcc
     8881 gcggccatcg tttcctgtcg ccggggtgga tcgagatcgc gagcccggac aagtacgtcg
     8941 atgcactgcg cgaggcgcac gtcgtcgtcg atccggacgc gcggcgggac ctcgtgcggg
     9001 ccgagctctc gcgcatcgag aaggatacga agctccgcgt ccgtcccgac gaggcgctga
     9061 tcaatgaggt gatcaacctc ggcgagtatc cggtcggcgt cagcggcacg ttcgatccgt
     9121 cgttcctcga agtgcccgaa gagatcatcg tcacgacgat gcgcaacaac cagcgctact
     9181 tcgcgatgga agacggaagc ggcaagcttg cgccacgctt cgtcacgatg atggcgacga
     9241 tcgtcaagga tccggccgtc gtcgcgcgcg gcaacgagta cgtgatcgcg tcgcgcctcg
     9301 cggacgcgaa gttcttcttc gccgaggacc gcaagaagac gttcgacgac tggaacgcga
     9361 agctcgacgg cgtcgtgttc caggccaagc tcggcgacgg cgcgaagacg atcggcaaca
     9421 aggttgcgcg catcgccggc atcgtcgaag cgctcggcgg ctcagcgacc gcaaagcaag
     9481 ccgcgcgcta cgcgaagagc gacctggcat cgctcgccgt cggcgagttc cccgaggtgc
     9541 agggcatcat ggggcgccac tacgcgaagc tcgccggtct cggcgatggc gtcgcgaccg
     9601 tgatcgatca gcactggtgg ccgaaggggc agggcgccgc actgccgacc accgacgacg
     9661 ccgctctcgt cgcgctcgcg gatcgcatgg acacgatcgt cggctgcttt gccgtcggtc
     9721 tcgagccaag tggctcggcg gatccgttcg gcctgcgccg cgccgccatc ggcatctggc
     9781 agatcctgct cgaccgcggc tggacgcagc actacgcgcg cctgcgccag gccgccgaga
     9841 cgcagctcgt ggcgcaaggc gtgaagctga aagacggcaa gcctctggac gagttcttcc
     9901 gcacccgcct gcgtggaatc tttgtcgagc aagacatccc gccgcaggac gccgacgcgg
     9961 ccctcgcgca ggactaccgc gatccaatcg atgcacgcgc ccgtgcactc gcgtgcgcga
    10021 agatcagcaa agaagcgcgc gaggtgttca agcgcgtcgc gaacatcctc gacgatgccc
    10081 gcggcaagaa gctcacgatc ggcgcgtcgc cggatccgtc gctcttcgtc gcgaaggtcg
    10141 agaccgacct ccacgccgcg atc
//
LOCUS       DASUFX010000640        17624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_242437,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000640 DASUFX010000000
VERSION     DASUFX010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242437"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2429)
                     /locus_tag="VFV99_25830"
     CDS             complement(<1..2429)
                     /locus_tag="VFV99_25830"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5062830.1"
                     /translation="MSREFAFPGTRLRFAPDRVVDIQHIALTLDVDPSKRTVSGNATL
                     RAMVIAPNTKAVELDAVELKIDKVTAAGKQVPFRHDGKRLRVELPSALATGAELTLSI
                     DYTGAPRRGLYFVGPDDGYPHKPLQVWTQGQDEDSRYWFPCFDAPSEKATSEVKVSVP
                     ATMFALSNGTLVSDKTSGAKRTLHWRLDVPHSCYLITLAVGDFGTVETKWHDTPVVYY
                     VERGREEAAERTLARTPQMLELFSQKFGVPYPYPRYAQVFVADFIFGGMENTSATTLT
                     DTVLIDERAAIDYDIDALVAHELAHQWFGDLVTCRDWGEGWLNEGFATYSEYIWREHH
                     EGRDAADLELEEWADQYFGEDAGRYRRTIATKLYDEPIDIFDHHLYEKGGRVLHMLRN
                     VLGDDAFYKSLAHYLGKHRHGLVESRDLARAVEDATGKVLDWFFSQWVIDGAGHPELD
                     IHMRWDPDTQLATVAIEQTQRVEGRTPVFRLPTTMRFRVNGKDVDVPLEIVDQKHVFH
                     LRLDAEPSQAIFDPGRVLLAARKIDKPEPVWIEELAGATLAVDRAAAAQALARRGGPA
                     AEQALMTALEKDKFWAVRGSAALGLAMLRTPAARDRLVKRLKAEVQPRARRIIARALG
                     EFVSDPAAGTALAAIVEKGDASYFVEAEACLALGRTRTPRAGELLRQAATRESFTDVI
                     RQHAYRGLAEARDDSALGLLAEGTKWGRPTQGRRAAAGALALLMRGRRDREARDVRER
                     LELLLADRDFRVQAAAVEALSVIGDPTAIPALRRMIDRELDGRLRRRGKEVVRDLGEG
                     APLAEDVRRLR"
     gene            complement(2494..3030)
                     /locus_tag="VFV99_25835"
     CDS             complement(2494..3030)
                     /locus_tag="VFV99_25835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062831.1"
                     /translation="MKRLIASPSAWLVTAACSAHAPTPVANEATTTPNYHDSVIAEWS
                     VANDGSSVTLAVRIYDQRVALGTYRGRVFPAWQTYCDVKMGYLPARNIDYAKLKGAIA
                     KLTVVGDGAASSEEPTPAGSTSFFLKRPSPNRLEVWKQDTRLAVVAVWPNAHFDEEID
                     DDDDKPGFNCGGDDGMNI"
     gene            complement(3005..3448)
                     /locus_tag="VFV99_25840"
     CDS             complement(3005..3448)
                     /locus_tag="VFV99_25840"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX5062832.1"
                     /translation="MIWVTKRRIKVNRAATAWLIRRFIDPQGEIRFVAPDEVERVQRE
                     EGALGFDAPNATYPHKDNRGRCSFEALVEQHVPHDPALARMARIVHCADFRDEIDLVP
                     EARGLRAISDGFPLVARDDDDTVSRASFLYDAMYAALDEEAHRIA"
     gene            complement(3465..4673)
                     /locus_tag="VFV99_25845"
     CDS             complement(3465..4673)
                     /locus_tag="VFV99_25845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5062833.1"
                     /translation="MPTPIKIASRLDPIKPSITLAVTAKAAKLKADGIDVVSFGAGEP
                     DFDTPDHIKDAARAALDKGVGKYTEVGGILPLRKAIAAELSAVHKTTIEPDQILVSSG
                     AKHSLYNLFMALIDPGDEVLIPAPYWVSYPDMVMLAGGRPVILETKAEDDFAVTAAQV
                     AAACTGRTRAIVLNNPSNPTGAVYTRAQIEALAKVVVEKDLLVISDDIYRQLVYGDAE
                     YVSIAAVSPELAKRTILVDGVSKTYAMTGWRIGYTAGPLPLIKAMAKIQGQSTSNPTH
                     IAQIATLAALTGPQECVGTMRKAFDERRKKMVELLRAIPGVKCREPKGAFYAFPDVST
                     YVGKKTPEGSILDDDVQLCDWLVEVGKVAVVPGSGFGAPGYVRLSYACSMKNIEDGVG
                     RLAKALASLK"
     gene            complement(4679..5188)
                     /gene="coaD"
                     /locus_tag="VFV99_25850"
     CDS             complement(4679..5188)
                     /gene="coaD"
                     /locus_tag="VFV99_25850"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5062834.1"
                     /translation="MTKDAVTTIAVYPGTFDPITNGHLDILGRALRLFDKVIVTIAIN
                     QRKQPLFTAEERIQFIHDAMPNAGDRLQFETFDGLLVDFARAHNATVIVRGLRALADF
                     EYEFQFAHMNRRLAPGIDSVFFMTDERNHYVSSSLVKEVASFGGDVTGLVPAPVVAAL
                     AAKYKSERK"
     gene            complement(5185..5724)
                     /gene="rsmD"
                     /locus_tag="VFV99_25855"
     CDS             complement(5185..5724)
                     /gene="rsmD"
                     /locus_tag="VFV99_25855"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5062835.1"
                     /translation="MRIVGGSHGGRVLRAPHGAATRPTSEKVRQAIFNILPDVEGMQV
                     LDMFAGSGALGIEALSRGAAHVTFIDQAKPALSAVRENLKDLDLEARATVLAGDAVTL
                     AARHVPASPWQLVFIDPPYRSDLAVRAVQTLAHLAPDAMIVIEHDRRNAPPDELRSLV
                     RTDQRRYGDTLVSFYRVVT"
     gene            5790..7370
                     /locus_tag="VFV99_25860"
     CDS             5790..7370
                     /locus_tag="VFV99_25860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX5062836.1"
                     /translation="MVGNLAAWLIEGALAKGAGPRTAVREGGRAWTYDELADHVARTS
                     AALRGLRLQRGERVLILMRDTLEAAAAILGTIHAGAIAVPISELATPDDVQDYVQHAG
                     AVIAVTDGAHEAVLDAIRNETPDMRDVVCVGAKLAGSHDFHKLVEGAQPQPAAPSGEE
                     DPALLLYSAGSGPGELRAVPHSKRTVQAAFQSFAQELLQLTDQDRILCIARLSTAYGL
                     GSGLLLPLATQAESLLFPEQPQSDKLFKVLDQFKPTVLFATPSVYAQLAHDAEKAQRT
                     KALASLRNAVAGAEGMPERLIPRIRSVLGTEVVVGYGLTEILQFALAGRSDDPGARPG
                     VCGKPLTGVQVRLIDEDGDPVGVDEIGTLELQCGSLFTGYWGGGTGNHDLRSDGWFTT
                     RDRFMIDREGFYHHCGRVDDLFKVGGKWVSPSEVERALTAHEGVWECAVIGADDDEGL
                     IKPLAFVVTNVGWEGGPKLEAELREYVKQTLAPYKYPRWIEFIESLPRGPGGKLLRYK
                     LRPQRRRRRAETGSHELP"
     gene            7367..8146
                     /locus_tag="VFV99_25865"
     CDS             7367..8146
                     /locus_tag="VFV99_25865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062837.1"
                     /translation="MSDADPVGGALAIAAERLGEHARAVLSRLGGQLGHDARVSHEQL
                     ATSDGAEGQRKRALIKVATRSPVPASLRTTHPTWIEAVVAELGERARTAVAAPSMNAV
                     DVWLARWATAALPPTLYDSLLPPVAATNDASVIVAWLGRIGADQMAFALGEQAKAVPA
                     LATAIARISKPPRAGELGPKRAAIERCRDVSLDDDFSFVIVACRALAPHLAANQLARL
                     QLILRLPRPIGIVVARELAAHAATSFDQCPSWSAFDADWCL"
     gene            8474..10030
                     /locus_tag="VFV99_25870"
     CDS             8474..10030
                     /locus_tag="VFV99_25870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX5062838.1"
                     /translation="MKAGRVIDGDEHDARTLAQQLVDDARAEADKLTEAARIEAAQLR
                     NDVQALASSILADARAEAERVSAAAQADAARLRDDAKVLANQLVQELRGETAPSVAVP
                     AETTGRVDEVVGLIVRATLPGVSLGEVVKIDRRGREPLVAEVVGFRGEQAVLLPLGDL
                     AGVAPASAVWRTGEALAIRCGDDLLGRVLDGVGQPIDGGAPLSGERWPVDRAAPSALA
                     RPPIRAPQATGVRAIDTMLTLGRGQRVGLFAAAGVGKSTLLGQIARGTSADVIVLCLV
                     GERGRELGELLGDELAPARGRTVVVCATSDAPPLVRLRAVHAATAVAEWFRDKRGASV
                     LLLVDSLTRVARAQREVGLSAGEPPARHGYPPSVFALLPRLVERAGATRDGAITAIYT
                     VLVAGNDMDEPIADEVRGLVDGHIVLDRRLAQRGHFPPIDVVASVSRLMPKLVDAQHS
                     AAAARVRERLATYEEHRDLITLGAYQTGRDRKVDDAVTAQPAIEMLLRQRRDESADWD
                     KALAHLLTLG"
     gene            10331..13402
                     /locus_tag="VFV99_25875"
     CDS             10331..13402
                     /locus_tag="VFV99_25875"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5062839.1"
                     /translation="MTPIGPVPFVGRQSELAELKQHLSLVSLVSLHGALGSGKTRLVT
                     ELAPTLGAPCTVIDCHPGDRASALRARAERALRCVPGTLAQTLAQQSRVLVIDDIQHL
                     RIDEATSLLAPLLLGPTALGRIVVVGRDPLSAAVGTSIAELELHGLDVEAAGALWTSL
                     EEQFGAAEGFDVAFGRTRGVPLALRREFARARFGANAWDIPSLDEPSRIAIEVLAVLR
                     QPVAPAAVGALAPQVDIEAALTSLVAHQLVDAVGDGRVVLHEVVRVDTLAQIAPEQRK
                     KLSAAAAELVASTAAVSKGPRLAWQAGDDGAFGAMDQITRVREVVWHWLAAGEREKGA
                     DVLLAHRELAARSGGAGEFEALLDALGDRRRRPGTHSEIADAGPLEPERARKLAALRI
                     EIAVRGGRFTEAFERATAAPGAVSPLLHAELTLASGDAAGARRALQGLIGSQDQSERA
                     RAIAGLAELELLAGKPDSAAAQLGSIGDVSNADPLARARVHLAAARADEYAGRVAAMR
                     SALSRAHGACKSVPACLEASELCAIVDARRAVGLAREGRLVEAATALDAADTAARDLD
                     AVAVADEVLAARALVARRRGDSGQAASLLHDLVRARRERGDELGALRAELDLAELEIL
                     RGQPALAAELASAALASSVRRELGHLAARGGAVVGAIDLMELRLDAALPRLEELHGQP
                     ALDASTSAQVAVLLATARALSGQRAGAIELAQNAGAESRDELDRRLAAAEVALATGDV
                     GHALELARDTAVLSERAHRTIELASALVIVARLELARGDRGNARAAATRAAREAAAAG
                     LIRVRIHALLALSALARDDDDAASAVAYARDASELALTAGLPVERLVAHAALDAISGH
                     EAVADPSAPSAATMAPSAIEGAARLLTDLGLTAQRPFRVIDAEGVPSDVADANPEILR
                     LPGRALAVDGVREVIWRHGQELADLRRRSLLKRLLFLFASAPGKVFSKEAIVQAVWNV
                     EYHPLRHDAALFTNIMRIRRLLGEDGSEIIRVTEDGYRFVPPRDFLFVIPR"
     gene            13399..13974
                     /locus_tag="VFV99_25880"
     CDS             13399..13974
                     /locus_tag="VFV99_25880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062840.1"
                     /translation="MKAKAKRKTGRSTRKATSTKRARPKPRATKKAAKQAKKPKARKA
                     GASRWVKFVEKHGIVLASAKGPVPNVAEAVAGEPIVGSWWAHAKGKAIFAALSEIDDS
                     TDVRCFKLVDGKVTFAHRKVWPALVRLGKEGVISAEQLAAVQQEHMPTGEHRNIVTPF
                     PDWVDDATAAAAEKLAADKARAQLAWIDASN"
     gene            complement(14327..14773)
                     /locus_tag="VFV99_25885"
     CDS             complement(14327..14773)
                     /locus_tag="VFV99_25885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5062841.1"
                     /translation="MVRISAKAVIIRDEQLLVTVHRDDQGDYYLLPGGGQERFEPLHD
                     TLRRECREEIAADIEVGELLFVRDYIGRNHEFAATDDAHQVEIMFACRVPDDYVPQSG
                     AIPDPRQHGATWLPLATLASARLYPAALKPLLVARSKTPMYLGDVN"
     gene            complement(14783..15199)
                     /locus_tag="VFV99_25890"
     CDS             complement(14783..15199)
                     /locus_tag="VFV99_25890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062842.1"
                     /translation="MVTRLVTASLVFAIAIADAAPVIEVRATCAAELASEPAHLRTLR
                     GELAQALARTTATASYTLDVSLVRLGVATVGRELEVRAEVRAMLSDAHGKIRWSSTSR
                     SAARGPAADRIRLQRDAVSAAARDLARSVRAHCCTR"
     gene            complement(15272..16327)
                     /locus_tag="VFV99_25895"
     CDS             complement(15272..16327)
                     /locus_tag="VFV99_25895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062843.1"
                     /translation="MTRLAFVVALALASVTGFGGRADAVPLERLPGTGRVEVSCEPGL
                     EATARELADSAEAELARISADLVDLPTPRRIRIQVVRDAESLSSVAPEGRGAPPYAIG
                     VAYPDLGIISVATRRGAQLVDPAGTLSHELAHIALGSALGPRAPRWLHEGFAYQHSAE
                     WSWDRTETLAGMTWFGGVIPIDQLDVSFPAEESPAHRAYAESYDFVGYLSRRGRWEDK
                     DDDGDRWPFRRFLTMVGHGTDIDVAATRAFGKPIGQLFDEWREDLSKRYMMAPIGLLG
                     LALWVFCALLLALAWLRKRRINRRRLGEWERQERREDEDVARLRPIIVPPYVPWPGED
                     PFADVDEDKPDPPKLVN"
     gene            complement(16324..17517)
                     /locus_tag="VFV99_25900"
     CDS             complement(16324..17517)
                     /locus_tag="VFV99_25900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062844.1"
                     /translation="MGPQQVLRVAACLLLVGGVASARERVAVIDLGPDDGTRQKLAAA
                     IVAGGLEPVIGDGVEDALAGINGDRDTVALAAALAEAKDKFGALACPETIAAAQTAIS
                     IGAARQAAGLPVPELARAWTYVLLCADRTNDASAAFAAATAIRTLGGSSAAAGDVDAK
                     LLAKYPDIDALSNREVMEVEIKADVDGADVFLDFRPVGKSPLKLVMATGPHIMAAAAG
                     GKRGTVTGTVIRKQPLVTIPMQDQSSKWSSLAMRVASWHGKVPKPKEIGWALGQVDAR
                     IALVRHGDTVEAWGHPGEGEPVRRLGGDDGSRKLDEAPALVALVVDRTQSWVDRAPDP
                     DQPLLTETPEERKARGHLKKGEEEEPTRWWVYATIAGAIVAGSIVIYAHNQADDTQRV
                     ELKYP"
BASE COUNT         2765 a         5777 c         6159 g         2923 t
ORIGIN      
        1 tcacgaagcc ggcgcacatc ttcggcaagc ggcgcacctt cgccgagatc gcgcacgact
       61 tccttgccgc ggcggcgcag gcggccgtcg agctcgcgat cgatcatgcg acgcagggca
      121 gggatcgcgg ttggatcgcc gatcaccgag agcgcctcga ccgcggcggc ttgcacacgg
      181 aagtcgcggt cggcgagcaa gagctcgagt cgttcgcgga catcgcgtgc ttcgcgatcg
      241 cggcggccgc gcatgagcag agcgagcgca ccggcggctg cgcgccggcc ttgcgtcggg
      301 cgtccccact tcgtgccttc ggcgagcagg ccgagtgcgc tgtcgtcgcg cgcttcggcg
      361 agcccgcggt atgcgtgctg gcgaatcacg tcggtgaacg attcgcgcgt cgccgcttgg
      421 cgcaacagct cgccggcgcg tggcgtccgc gtacgaccga gcgcgaggca cgcttcggcc
      481 tcgacgaagt agctggcgtc gcccttctcg acaatggcag cgagtgcggt acccgcagcc
      541 ggatcgctca cgaactcgcc gagggcgcgc gcgatgatcc gccgagcgcg tggctggact
      601 tcggccttga ggcgcttcac gagtcgatcg cgagcggctg gcgtgcggag catcgcgagc
      661 ccgagcgcag cgctgccgcg cacggcccag aacttgtcct tctcgagcgc ggtcatcagc
      721 gcttgctccg cggcgggccc accgcggcgt gcgagtgctt gcgcggcggc ggcgcgatcg
      781 accgcgagtg tggcgccggc tagttcctcg atccagaccg gctctggctt gtcgatcttg
      841 cgcgctgcga ggagcacgcg gcccggatcg aagatcgcct gtgacggctc cgcgtcgaga
      901 cgcagatgga acacgtgctt ctggtcgacg atctcgagcg gcacgtcgac atccttgccg
      961 ttgacgcgga atcgcatcgt cgtcggcagg cggaacaccg gcgtgcggcc ttcgacccgt
     1021 tgcgtctgct cgatcgcgac ggtcgcgagc tgggtatcgg gatcccagcg catgtggatg
     1081 tcgagctcgg gatggccggc tccatcgatc acccactgcg agaagaacca atcgagcacc
     1141 ttgccggtcg cgtcctcgac ggctcgcgcg aggtcacgcg actcgacgag cccgtggcga
     1201 tgcttgccga ggtagtgcgc gagcgacttg tagaacgcgt cgtcgccgag cacgttgcgc
     1261 agcatgtgga ggacgcggcc gcccttctcg tagaggtggt gatcgaagat gtcgatcggc
     1321 tcgtcgtaga gcttcgtcgc gatcgtgcgg cggtagcggc cggcgtcttc accgaagtac
     1381 tggtcggccc attcctcgag ctcgaggtcg gctgcgtcac ggccttcgtg gtgctcgcgc
     1441 cagatgtact cggagtacgt cgcgaagcct tcgttgagcc agccttcgcc ccaatcgcga
     1501 cacgtgacga gatcgccgaa ccactggtgc gcgagctcgt gcgcgacgag cgcgtcgatg
     1561 tcgtagtcga ttgcggcgcg ttcatcgatc aagacggtgt cagtgagcgt cgtcgcactc
     1621 gtgttctcca tgccgccgaa gatgaagtcg gcgacgaaca cctgcgcgta gcgcggatac
     1681 ggatacggca ccccgaactt ctgcgagaac agctcgagca tctgcggcgt gcgcgcgagc
     1741 gtgcgctcgg cggcttcctc acggccgcgc tcgacgtagt agacgaccgg cgtgtcatgc
     1801 cacttcgtct cgacggtgcc gaagtcgccg accgcgagcg tgatcaagta gcagctgtgc
     1861 gggacgtcga ggcgccagtg cagcgtgcgt ttggcgccgc tcgtcttgtc gctgacaagc
     1921 gtgccgttcg acagcgcgaa catcgtcgcc ggcacgctga ccttgacctc actcgtcgcc
     1981 ttctcgctgg gcgcatcgaa gcacggaaac caatagcggc tgtcttcgtc ctggccctgc
     2041 gtccatacct gcagcggctt gtgcggatag ccatcgtcgg gaccgacgaa gtacaggccg
     2101 cggcgtggcg cgcccgtgta gtcgatcgat agcgtgagct cggcgccggt cgcgagcgcg
     2161 ctcggcagct cgacgcgcaa gcgcttgccg tcgtggcgga acggaacctg ctttccggct
     2221 gccgtcacct tgtcgatctt cagctcgacg gcgtcgagct cgacggcctt cgtgttcggc
     2281 gcgatgacca tcgcgcgcaa cgtcgcgttg ccgctgacgg tgcgcttgct gggatcgacg
     2341 tcgagcgtca gcgcgatgtg ttggatatcg acgacgcgat cgggcgcgaa ccgcagccgc
     2401 gtacccggga aggcgaactc gcgagacatc tgcgcgcaat gtagcggaag tcgctaacgc
     2461 gcacgacggc gcaacgcccc gatcacggcg gttctagatg ttcatgccgt cgtcgccgcc
     2521 gcagttgaag cctggcttat cgtcgtcgtc gtcgatttct tcgtcgaagt gcgcgttcgg
     2581 ccataccgcg acgaccgcga ggcgtgtgtc ctgcttccag acctcgaggc gatttggcga
     2641 tggtcgtttg aggaagaacg aggtcgagcc ggcgggagtc ggctcctcgg acgacgccgc
     2701 gccgtcaccg acgacggtga gcttcgcaat cgcgcctttg agcttcgcgt agtcgatatt
     2761 gcgcgccggc aagtagccca tcttgacgtc gcagtaggtc tgccacgccg gaaacacgcg
     2821 gccgcgatac gtgcccagtg cgacgcgctg gtcgtagatg cgaaccgcga gagtcacgct
     2881 gctgccgtcg ttcgcgaccg accattcggc gatgaccgag tcgtggtagt tcggtgtcgt
     2941 cgtcgcctcg ttcgcgaccg gtgtcggcgc atgcgcgctg catgcggccg tgaccagcca
     3001 cgcgctaggc gatgcgatga gcctcttcat cgagcgccgc atacatcgcg tcgtagagga
     3061 agctcgcgcg cgagacggtg tcgtcatcgt cacgcgccac gagcgggaaa ccgtcggaaa
     3121 tcgcgcgcag gccacgtgcc tcgggcacga gatcgatctc gtcacgaaaa tcagcgcagt
     3181 gaacgatgcg cgccatccgc gccagcgccg gatcgtgcgg cacgtgctgc tcgacaagcg
     3241 cttcgaacga acaccggccg cggttgtcct tgtgcggata tgtcgcgttc ggcgcgtcga
     3301 agccgagtgc gccttcttcg cgctgcacgc gctcgacctc gtcgggggcg acgaaccgaa
     3361 tctcgccctg gggatcgatg aagcggcgaa tcagccatgc cgtcgccgca cggttgacct
     3421 tgatgcgccg cttggtgacc cagatcatga gtcaaactgt agcgctactt cagcgaagcg
     3481 agagctttcg ctagacggcc cacaccgtcc tcgatgttct tcatcgaaca tgcgtaggac
     3541 aggcgtacgt aaccaggcgc accgaagccg gatccgggca caaccgcgac cttaccgacc
     3601 tcgaccagcc aatcgcacag ttgcacgtcg tcgtcgagga tcgagccttc cggcgtcttc
     3661 ttgcccacgt acgtcgagac gtcggggaac gcgtagaacg cgcccttcgg ctcgcggcac
     3721 ttcacgccgg gaatcgcgcg cagcaactcg accatcttct tgcggcgctc gtcgaacgcc
     3781 ttgcgcatcg tgccgacgca ctcctgcgga ccggtgagcg cggcgagcgt cgcgatctgc
     3841 gcgatgtgcg tcgggttcga cgtcgactgg ccctggatct tcgccatcgc tttgatcagc
     3901 ggcaaggggc ctgcggtgta gccgatacgc cagcccgtca tcgcgtacgt cttgctgacg
     3961 ccgtcgacga ggatcgtgcg cttggcaagt tcggggctga cggctgcgat cgagacgtac
     4021 tcggcatctc cgtagacgag ctggcggtag atgtcgtccg agatcacgag cagatctttt
     4081 tcgacgacaa ccttcgcgag cgcttcgatc tgcgcgcgcg tgtagaccgc gccggtcggg
     4141 ttcgacgggt tgttgagcac gatcgcgcgc gtgcgcccgg tacacgcggc ggcgacttgc
     4201 gctgcggtga ccgcgaagtc gtcctctgcc ttcgtctcga ggatcaccgg gcgaccgccg
     4261 gcgagcatca ccatgtccgg atagctgacc cagtacggcg ccgggatgag cacctcgtcg
     4321 cccgggtcga tgagcgccat gaacaagttg tagagcgagt gcttcgcacc actcgacacg
     4381 aggatctggt cgggctcgat cgtcgtcttg tggacggcgg acagctccgc cgcgatcgct
     4441 ttgcgcagcg ggagaatgcc gccgacctcg gtgtacttgc cgacgccctt gtcgagcgcg
     4501 gcgcgcgcgg cgtccttgat gtgatcgggc gtgtcgaagt caggctcgcc ggcgccgaag
     4561 ctgacgacgt cgatgccgtc ggctttgagc ttggccgcct tcgcggtgac cgcgagcgtg
     4621 atcgatggct tgatcggatc gaggcggctg gcaatcttga tcggagtcgg catgggcttc
     4681 acttcctttc gctcttgtac ttcgcggcga gcgccgcgac cacgggagca gggacgagac
     4741 cggtgacatc gccgccgaag ctggcgactt cttttacgag ggacgacgag acgtagtgat
     4801 ttctctcgtc ggtcatgaag aacaccgagt cgataccggg ggcgagccgc cggttcatgt
     4861 gcgcgaactg gaactcgtac tcgaagtcgg cgagggcgcg caggccgcgg acgatcacgg
     4921 tcgcgttgtg agcacgcgcg aagtcgacga gcaggccgtc gaacgtctcg aactgcagcc
     4981 gatcgcccgc gttcggcatc gcgtcatgga tgaattgaat ccgctcctcg gccgtgaaca
     5041 gcggctgctt gcgctggttg atcgcgatcg tgacgatcac tttgtcgaac agacgcagcg
     5101 cgcgcccgag aatatcgagg tggccgttcg tgatcgggtc gaacgtgccg ggatagaccg
     5161 cgatcgtggt cactgcatct ttggtcatgt caccacccga taaaacgaga ccagagtgtc
     5221 gccgtaacga cgttggtccg ttcgtaccag agatcgaagc tcgtcgggag gcgcgttgcg
     5281 gcgatcgtgc tcgatgacga tcatggcgtc gggggcgagg tgagcgagcg tctgcacggc
     5341 gcgaaccgca agatcgctgc gatacggcgg atcgatgaag acgagctgcc agggtgacgc
     5401 cggtacatgt cgcgccgcca gtgtcaccgc gtcgcccgcc aacaccgtcg cgcgggcttc
     5461 gagatccagg tctttgaggt tctcgcgcac cgcactgagc gcaggttttg cctggtcgat
     5521 gaacgtcacg tgcgccgcgc cacgtgacag cgcttcgatg ccgagcgccc cggatcccgc
     5581 gaacatgtcg agcacctgca tgccttcgac atccggcagg atgttgaaga tcgcctgtcg
     5641 gactttctcc gaagtcggcc gggtcgcggc gccgtggggg gcacggagca cccggccgcc
     5701 atggctaccg ccgacgatgc gcatctgctc aggaatgtac gacttctttt tgttctcgcc
     5761 cccgtactcc gatacgatta cgcacgtcta tggtggggaa cctagcggca tggctaatcg
     5821 agggagcgct cgcgaagggc gctggccccc gcaccgccgt gcgtgaaggc ggccgcgcct
     5881 ggacctacga cgagctcgcc gatcacgtcg cgcggacgtc ggccgcgcta cgcggattac
     5941 ggctccaacg cggtgagcgc gtgctgatcc tgatgcgcga tacgctcgag gcagcggctg
     6001 cgatcctcgg cacgatccac gctggcgcga tcgcggttcc gatctcggag ctcgcgacgc
     6061 ccgatgacgt ccaggactac gtgcagcacg ccggcgcggt gatcgcggtg accgacggtg
     6121 cgcacgaagc ggtgctcgat gcgattcgca acgagacgcc cgatatgcgc gatgtcgtgt
     6181 gcgtcggcgc gaagctcgct ggcagtcacg acttccacaa gctcgtcgag ggcgcacagc
     6241 cgcaaccggc ggcgccgagc ggggaggaag atccggcgct gctgctgtac tcggccggtt
     6301 cgggtcccgg cgagctgcgc gcggtgccgc attcgaaacg gacggttcag gcggcgtttc
     6361 aatcgttcgc gcaggagctg ctgcagctca ccgatcagga tcgcatcctg tgcatcgcgc
     6421 gcctctcgac ggcatacggt ctcggctcgg gcctcttgtt gccgctcgcg acgcaagcgg
     6481 agtcgctgct gttccccgag caaccgcagt ccgacaagct gttcaaggtt ctcgatcagt
     6541 tcaagccgac cgtgttgttc gcgacgccgt cggtctacgc gcagctcgcg cacgacgccg
     6601 agaaggcgca gcgcacgaag gcgctcgcga gcttgcgcaa cgcggtcgcc ggtgcggagg
     6661 gtatgcccga gcgcttgatc ccgcgcattc gcagcgtgct cggcaccgag gtcgtcgtcg
     6721 gttacggcct caccgagata ttacagttcg cactcgcagg acgcagcgac gatccgggcg
     6781 cgcgcccggg cgtgtgcggc aagccactca ccggcgtaca ggtgcggttg atcgacgagg
     6841 acggcgatcc ggttggcgtc gacgagatcg gcacgctcga gctgcagtgt ggctcgctgt
     6901 tcaccggcta ttggggcggt ggcaccggca accacgacct gcgcagcgac ggttggttca
     6961 cgactcgcga tcgcttcatg atcgatcgcg agggcttcta tcaccactgc ggccgcgtcg
     7021 acgatctgtt caaggtcggc ggcaagtggg tgtcgccatc cgaagtcgag cgcgcgctga
     7081 ctgcgcacga aggcgtgtgg gagtgcgcag tgatcggagc cgatgacgat gagggtctga
     7141 tcaagccact cgcgttcgtg gtcacgaacg tcggctggga gggcggtccc aagctcgagg
     7201 ccgagctgcg cgagtacgtg aagcagacgc tcgcgccgta caagtacccg cggtggatcg
     7261 agtttatcga gtcgctcccg cgtggcccgg gcggcaagct gctgcgctac aagctgcgcc
     7321 cgcagcgtcg ccgccgtcgc gccgagaccg gttctcacga gctgccgtga gcgacgccga
     7381 cccggtcggc ggtgcgctcg cgatcgcggc cgaacggctc ggcgagcacg cacgggctgt
     7441 gctgtcacgt ctcggcgggc agctcggcca cgacgcgcgc gtctctcacg agcagctggc
     7501 caccagcgac ggcgcggagg gacaacggaa gcgggcgctg atcaaggttg ctacgcgatc
     7561 gccggtaccc gcgtcgctgc gcaccacgca tccgacgtgg atcgaggcgg tggtcgccga
     7621 actgggcgag cgtgcgcgca cggcagtcgc cgcgcccagc atgaatgccg tcgacgtgtg
     7681 gctcgcgcgc tgggcaacgg ccgcgctgcc accgacgctg tacgattcct tgctgccacc
     7741 cgttgccgca acgaacgacg ccagcgtgat cgtcgcgtgg ctcggtcgca tcggcgcgga
     7801 tcaaatggcg ttcgcgctcg gcgagcaggc gaaggcggtg cctgcgctcg cgaccgcgat
     7861 cgcgcgcatc agtaagccgc ctcgcgcagg cgagctcggc cccaagcgcg ccgcgatcga
     7921 gcgttgtcgc gatgtctcgc tcgacgacga cttttcgttc gtcatcgtcg cgtgtcgagc
     7981 gctggcgcca catctcgcgg cgaatcagct cgctcgcctg caactgatcc tgcgcttgcc
     8041 acgaccgatc ggaatcgttg tcgcgcgcga gctggcggca cacgccgcga catcattcga
     8101 tcaatgcccg agctggtctg cgttcgacgc agactggtgc ctgtgagcgc gcgggcccgc
     8161 ggattgcaaa ccggcgagcc atgccaacga tgcgagagaa agcaaaagca cgaagcgaga
     8221 agatgtccgg caaagcgcgc gatgcgaagg cctcatccag tagccggatg aagtcggcca
     8281 aggagcgcgc gagcgccggg atgaaaaacg tcggtcgcaa gatcgaagac gcgggccagc
     8341 gcgcgaagtc caaggcgaag tcgcgccgtc ggcgctaggt cctcgcgtac tgcagagtac
     8401 ggctcgatcg aacgattcag cgcccgtgag gcctcgtcca cgcaggacgt atacgaggtg
     8461 atacgctgga tccatgaagg cgggccgcgt gatcgacggg gacgagcacg atgcgcggac
     8521 gctcgcgcaa cagctcgtcg acgacgcgcg cgccgaagcc gacaagctga ccgaagcggc
     8581 gcgcatcgag gcggcgcagc tgcgcaacga cgtgcaagcg ctcgcctcga gcatccttgc
     8641 cgatgcacgc gcagaggccg agcgagtctc ggcggccgcg caagccgacg ccgcgcgcct
     8701 gcgcgacgac gcgaaggtgc tcgcgaacca gctcgtgcaa gagctgcgag gcgagaccgc
     8761 gccgtcggtg gcggtgccgg cggagaccac gggccgcgtc gatgaagtcg tcggtctcat
     8821 cgtccgcgcg acgctgccgg gcgtgtcgct cggcgaggtc gtcaagatcg atcgccgcgg
     8881 gcgcgagccg ctcgtggctg aagtcgtcgg ctttcgcggt gagcaagccg tgctgcttcc
     8941 actcggcgat ctcgcaggtg tcgcgccggc gagcgcggtg tggcgtaccg gcgaggcgct
     9001 cgcgatccgc tgcggtgatg atctgctcgg ccgcgtgctc gatggtgtcg gccaaccgat
     9061 cgacggtggt gcaccgcttt ccggcgagcg ttggccggtc gaccgtgccg caccatctgc
     9121 cctcgcgcgg ccaccgatcc gtgcgccgca ggcgaccggg gtgcgtgcga tcgatacgat
     9181 gctcacgctt ggccgtggcc agcgcgtcgg gttgttcgcg gcggccggcg tcggcaagtc
     9241 gacgctgctc ggtcagatcg cgcgcggtac cagtgctgac gtgatcgtgc tctgcctcgt
     9301 cggcgagcgc ggccgcgagc tcggcgagct actcggtgac gagctggcac ccgcacgtgg
     9361 ccgcaccgtg gtggtgtgtg ccacgagtga cgcgccacca ctggtccgct tgcgtgcggt
     9421 gcacgcggca actgcagtgg ccgagtggtt ccgggataag cgcggcgcat cggtgctgct
     9481 gctggtcgat tcgttgacgc gcgtcgcgcg ggcacaacgc gaggtcgggc tgtcggccgg
     9541 cgagccacct gcgcgtcacg gttatccgcc gagcgtcttc gcgttgttgc cgcgactcgt
     9601 cgagcgggca ggagcgacgc gcgacggcgc gatcaccgcc atctacaccg ttctcgttgc
     9661 cggcaacgac atggacgagc cgatcgccga tgaggtgcgt ggcctggtgg atggccacat
     9721 cgttctcgat cgcaggctcg cgcaacgtgg acactttccg ccgatcgacg tcgtcgcgag
     9781 cgtgtcgcgt ctcatgccga agctcgttga cgcacaacac tctgctgctg ctgcgcgcgt
     9841 gcgcgagcga ctcgcgacct atgaagagca ccgagatctg atcacgctcg gggcgtatca
     9901 gacgggtcgc gatcgaaagg tagatgatgc ggtcaccgcc caacccgcca tcgaaatgct
     9961 tctccgccag cgccgcgacg agtcagcgga ctgggacaag gcgctcgcgc acctgctcac
    10021 gcttggctag cgatgctgtg ggcgtaatct tcccccaagt tttcggggta cccctagctg
    10081 gcactagctg acgccttccc tccccacgac cgggacttgt tgccccgatc gctaggcgat
    10141 cgttgcgatt ccaacgcatc acgtcacgtc aacattgcga gccggcccac aatcgaagac
    10201 catgcatcgc tgagttgacg cggacttcca tagaagccga tcgctacccc acactggttt
    10261 acatcttgct gacaccgatg gcgccatccc tatgcagacc cgccgtcgta gctcgaaaat
    10321 caccaaccca atgacaccta ttggaccggt gccgtttgtc ggtaggcaaa gcgagctggc
    10381 cgagctcaag cagcaccttt cattggtgtc gcttgtctcg ctgcacggcg cgcttggctc
    10441 tggcaagacg cgcctggtca cggagctcgc accgacactc ggtgcgccgt gcaccgtgat
    10501 cgattgccat ccgggagacc gggcatcggc actgcgagca cgcgcagaac gcgcactccg
    10561 ttgtgtgccg ggcacgcttg cgcaaacgct cgcgcaacag tcgcgagtac ttgttatcga
    10621 cgatatccag cacctgcgca tcgatgaggc tacgagcctg ctcgcgccgc tgctgctcgg
    10681 accgacggcg ctcggtcgca tcgttgttgt cggccgcgat ccgctgtcgg ctgccgtcgg
    10741 cacttcgatc gcagagctcg agctgcatgg cctcgacgtc gaagcagccg gtgcgctgtg
    10801 gacgtcgctc gaagaacagt ttggcgctgc agagggattc gatgtcgcgt tcggccgcac
    10861 gcgtggcgtg ccgctcgcac tgcgccgcga gttcgcgcgc gctcggttcg gcgcgaacgc
    10921 atgggatatc ccgtcactcg acgagccgtc gcggatcgcg atcgaagtgc tggcggtgct
    10981 ccgtcagccg gtggcgcctg cggccgtcgg tgcgctcgcg ccccaggtcg atatcgaagc
    11041 cgcgctgacc agcctcgtcg cgcatcagct cgtcgatgcg gtcggtgatg gtcgcgtcgt
    11101 cctccatgaa gtcgtgcgcg tcgatacgct cgcgcagatc gcgcccgagc agcgcaagaa
    11161 gctgtcggcc gccgcggcgg agctcgtcgc gtcgacggct gcggtctcga agggcccgcg
    11221 tctcgcgtgg caagccggtg atgacggtgc gttcggtgcc atggatcaga tcacgcgcgt
    11281 gcgcgaagtc gtgtggcact ggctcgccgc cggtgagcgc gagaagggcg ccgacgttct
    11341 gctcgcgcac cgcgagctcg ccgcccgttc gggtggtgcg ggcgaattcg aagcgttgct
    11401 cgacgcgctc ggcgatcgcc gccgccgtcc cggcacgcac agcgagatcg cggacgcagg
    11461 tccactcgag ccggagcgcg cgcgcaagct cgcggcgctg cgcatcgaga tcgctgtacg
    11521 cggtggtcgc ttcaccgaag cattcgagcg cgcgaccgct gcgccgggcg ccgtgtcgcc
    11581 gttgctccat gccgagctca cgctcgcgtc gggcgacgct gccggtgcac gccgcgcact
    11641 gcaaggtttg attggctccc aggatcagag cgagcgcgct cgcgccatcg ccggcctcgc
    11701 cgagctcgag ctgcttgccg gcaagccgga ttcggccgcc gcacagctcg gctcgatcgg
    11761 tgacgtctcg aacgcggatc cgctcgcgcg tgcccgcgtt cacctcgccg ccgcgcgcgc
    11821 cgacgaatac gccggccgcg tcgctgcgat gcgctcggcg ctgtcgcgtg cacacggggc
    11881 gtgcaagagc gtccctgcct gcctcgaagc gagcgagctg tgcgcgatcg tcgatgcacg
    11941 ccgcgcggtc ggtctcgccc gcgaagggcg cctcgtcgag gccgccaccg cgctcgacgc
    12001 cgccgacacc gccgctcgcg atctcgacgc ggtcgccgtt gctgacgaag tgctcgctgc
    12061 ccgtgcgctc gtcgcgcgtc gtcgcggtga cagtgggcaa gcggcatcgc tgctccacga
    12121 tctcgtgcga gcgcgtcgcg aacgcggcga cgagctaggt gccctgcgcg ccgagctcga
    12181 tctcgccgag ctcgaaatcc tccgcggcca gcccgcgctc gctgcagagc ttgcgtcggc
    12241 cgcgctggct tccagtgtcc gtcgcgagct cggccatctc gccgcccgtg gtggcgcggt
    12301 cgtcggcgcg atcgatctca tggagcttcg cctcgacgcg gcgctgcctc gcctcgaaga
    12361 gctgcacggc cagccggcgc tcgatgcgtc gacgtcggcg caggtcgcgg tgctgctcgc
    12421 cacggcgcgc gcgctgtccg gccaacgcgc cggagcgatc gagctcgcgc agaacgccgg
    12481 cgccgaatcg cgtgacgagc tggatcgccg tctcgcagcc gcggaagtcg cgctcgcgac
    12541 cggcgacgtt ggccacgcgc tcgagctggc ccgcgatact gcggtgctct ccgagcgcgc
    12601 gcatcgcacg atcgagcttg cctccgcgct cgtgatcgtc gcccgtctcg agctcgcccg
    12661 cggcgatcgc ggcaacgccc gcgcagcggc aactcgcgcg gcgcgtgaag ccgccgccgc
    12721 cggcctcatc cgcgtccgca tccacgcgct cctcgccctc tcggcgctcg cacgtgacga
    12781 tgacgacgcg gcgtccgcgg tcgcctacgc gcgcgacgcc tccgagctcg cgctcacggc
    12841 gggcctgccc gtcgagcggc tcgtcgcgca tgcggccctc gatgccatct ccggccacga
    12901 agcggtcgcc gatccgtcgg cgccgagcgc ggccacgatg gcaccgagcg cgatcgaagg
    12961 cgcggcccgc ctgctcaccg acctcggcct caccgcccag cgcccgttcc gcgtgatcga
    13021 cgccgaaggc gtgccgagcg atgtcgccga cgccaacccc gagatcctgc ggctgccggg
    13081 tcgcgcgctc gccgtcgacg gcgtccgcga ggtgatctgg cgccatggcc aggagctcgc
    13141 cgacctccgt cgccgctcgc tgctcaagcg cctgctgttc ctgttcgcgt cggcaccggg
    13201 caaggtgttc tcgaaggaag cgatcgttca ggcggtgtgg aacgtcgagt accacccgct
    13261 ccgccacgac gccgccctgt tcacgaacat catgcgtatc cggcgccttt tgggcgagga
    13321 tgggtcggag atcatccgcg tgaccgaaga cggctaccgt tttgtcccgc cgcgtgattt
    13381 cctattcgtc atcccgcggt gaaagcaaaa gcgaaacgga agacagggcg tagtacccgt
    13441 aaggcgacgt cgacgaagcg cgcgcggccc aaaccgcgtg cgacgaagaa ggcggccaag
    13501 caggcgaaga agcccaaggc acgcaaggca ggagcctcgc gttgggtgaa gttcgtcgag
    13561 aagcacggca tcgtgctcgc gtcggcgaag gggccggtgc caaacgtcgc cgaggcggtg
    13621 gcaggcgagc cgatcgtcgg tagctggtgg gcgcacgcga agggcaaggc gatctttgcg
    13681 gcgctgtcgg agatcgatga ttcgacggac gtgcgctgct tcaagctcgt cgacggcaag
    13741 gtcacgttcg cgcaccgcaa ggtgtggccg gcgctggtgc gcctgggcaa ggaaggcgtg
    13801 atttcggcgg agcagctggc ggcggtgcag caagagcaca tgccgacggg cgagcaccgg
    13861 aacatcgtga cgccgttccc cgactgggtc gacgatgcga cggcggcggc ggcagagaag
    13921 ctggcggcgg acaaagcgcg cgcgcagctc gcgtggatcg acgcgagtaa ctgaccgctt
    13981 cggcggcggg acgggagcag gaacgggaac gggaacagga acgggaacgg gaagaggaac
    14041 aggaacggga acgggaacag gatcgggaac ggtaagtggc tctgatccgc gcgattggga
    14101 accttgggat cccgaagata accagcgcgc aatgctcgac acgaagcgcg aattagcccg
    14161 ttggcctcgt cggtgcgcgc tcgccctgcg cgccgttgcg cgcacgcgtc gcgccaagcg
    14221 ctgaagcgct gcacgtagcg cgagccgccg ccgcgaagac gcgcgctgcg cagagcgcgg
    14281 ccactggcga gggcgcttga tccgttgtgc ccctgggggc gcgatttcag ttcacgtcgc
    14341 cgaggtacat gggcgtcttc gagcgcgcga cgagtagcgg cttgagcgcg gcagggtaca
    14401 ggcgtgccga agcgagcgtg gcgagcggca gccaggtcgc gccgtgctgc ctcggatcgg
    14461 ggatggcgcc gctctgtggc acgtagtcgt cgggcacgcg gcacgcgaac atgatctcga
    14521 cctggtgcgc gtcatcggtc gccgcgaatt cgtgattgcg tccgatgtaa tcgcgaacga
    14581 acaggagctc accgacctcg atgtcagcgg cgatttcttc gcggcattcg cggcgaagtg
    14641 tgtcgtgaag cggttcgaag cgttcttggc cgccgccggg aagtaagtag tagtcgcctt
    14701 ggtcgtcgcg gtgcacggtg acgagcagct gctcgtcgcg aatgatcacg gcctttgcgg
    14761 aaatcctgac cacgcgggga gactaccgcg tgcaacagtg tgcgcgcacg gaccgcgcga
    14821 gatcacgggc ggcggcgctg acggcgtcgc gctgcaggcg aatgcgatcg gcggcggggc
    14881 cacgcgcggc agagcgcgac gtcgacgacc agcggatctt gccgtgcgca tcggagagca
    14941 tcgcgcgaac ctcggcgcgg acctcgagct cgcgacctac cgtggcgacg ccgagacgta
    15001 cgagggatac gtcgagcgtg tagcttgcgg tcgcagtggt acgcgcgagt gcctgtgcga
    15061 gctcaccgcg cagcgtgcgg agatgcgccg gctcgctcgc gagctcggcg gcgcaagttg
    15121 cgcgaacttc gatgacagga gctgcatccg cgatcgcaat tgcgaagacg agagaggctg
    15181 tgacgaggcg cgtaaccatg gcgccgggta cagcaaggcg ggtgcctgag cggcggcgcg
    15241 cgcgcgtgct actgcgtgcg cgagagcgcg atcagtttac gagcttcggc ggatccggtt
    15301 tgtcttcgtc tacgtcggca aacggatctt cgccgggcca cggcacgtac ggcggaacaa
    15361 tgatcgggcg caggcgcgcg acgtcttcgt cctcgcggcg ctcctggcgt tcccactcgc
    15421 cgaggcgacg acggttgatg cggcgcttgc ggagccaggc gagcgcgagc agcagcgcgc
    15481 agaacaccca cagcgcgagg ccgagcagac cgatcggcgc catcatgtag cgcttcgaga
    15541 ggtcttcgcg ccattcgtcg aagagctggc cgatcggttt gccgaatgcg cgcgtggcgg
    15601 cgacgtcgat gtccgtgccg tggccgacca tggtaaggaa gcggcggaat ggccagcgat
    15661 cgccgtcgtc gtccttgtct tcccagcgac cacgacgcga gagatagccg acgaagtcgt
    15721 aggactcggc gtaggcgcgg tgtgccgggc tttcttcggc cgggaacgag acgtcgagct
    15781 gatcgatcgg gatgacgcca ccgaaccagg tcatgccggc gagcgtttcg gtgcggtccc
    15841 acgaccactc agcggagtgt tggtacgcga agccttcgtg cagccagcgc ggcgcgcgcg
    15901 gaccgagcgc actgccgagc gcgatgtgcg cgagctcgtg gctgagcgtg ccggccggat
    15961 cgacgagctg tgcgccgcga cgcgtggcga ccgagatgat gccgaggtcg ggataggcga
    16021 caccgatcgc gtagggtggg gcgccgcggc cttcgggcgc gacggacgac agcgattctg
    16081 cgtcgcgcac gacctggatg cggatgcggc gtggcgtcgg caggtcgacg agatcggcgc
    16141 tgatgcgagc gagctcggct tctgcactgt cggcgagctc gcgcgcggtc gcttcgagac
    16201 cgggctcgca gctgacctcg acgcggccgg tgccgggcaa ccgctcgagc ggcacggcat
    16261 cggcgcggcc accgaaccct gtgacgctgg cgagcgcgag cgcgacgacg aacgcgaggc
    16321 gcgtcatgga tatttcagct ccacgcgctg ggtgtcgtcc gcttggttgt gggcgtagat
    16381 gacgatcgag ccggcgacga tcgcgcctgc gatggtcgcg tagacccacc agcgcgtcgg
    16441 ttcttcttct tcgcccttct tgagatggcc gcgcgctttg cgctcttcgg gtgtttcggt
    16501 gagcagtggt tgatctgggt cgggcgcgcg atcgacccat gattgcgtcc gatcgacaac
    16561 gagcgcgacg agcgccggcg cttcgtcgag cttgcgcgaa ccatcgtctc cgccgaggcg
    16621 gcggactggt tcgccttcgc ccggatggcc ccacgcttcg acggtgtcac cgtggcgaac
    16681 gagcgcgatg cgggcgtcga cttgaccgag agcccacccg atctctttcg gcttgggcac
    16741 cttgccgtgc cagctcgcga cgcgcatcgc gagactcgac cacttgctcg attggtcttg
    16801 catggggatc gtgacgagcg gctgcttgcg gatgaccgtg ccggtgaccg tgccgcgctt
    16861 gccgccggca gccgcggcca tgatgtgcgg gccggtcgcc atcacgagct tgagcggcga
    16921 cttgccgacc gggcggaaat cgagaaagac gtcggcgccg tcgacgtccg ctttgatctc
    16981 gacctccatc acctcgcggt tcgagagcgc gtcgatgtct ggatactttg cgaggagctt
    17041 cgcatcgacg tcgccagcgg ctgcgctcga tccgccgagc gtgcggatcg ccgtggcggc
    17101 agcgaatgcg gcggatgcgt cgttcgtgcg atcggcgcag agcaagacgt acgtccacgc
    17161 acgcgcgagc tcggggaccg ggagaccggc ggcctggcgc gccgcaccga tcgagatcgc
    17221 ggtttgcgcg gctgcgatcg tttcggggca ggcgagtgcg ccgaacttgt ctttggcctc
    17281 ggcgagtgcg gccgcgagcg cgaccgtgtc gcgatcaccg ttgataccgg cgagcgcatc
    17341 ttcgacgccg tcgccgatga cgggttcgag gccgcccgcg acgatcgcag cggcaagctt
    17401 ctggcgcgtg ccgtcgtcag gaccgaggtc gatgaccgca acgcgttcgc gagcgctcgc
    17461 aactcctccg actaggagga ggcaggcagc gactcgaaga acttgttgag gccccatacg
    17521 tcacgtgcgc gaaggccgtc gaattgtaag ccggttgcct tggcgtcgga ccaacgaacc
    17581 gtcgcgccga cctcgatcgg atgctcgatc gtcgggatcg agaa
//
LOCUS       DASUFX010000641         6754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1491011, whole genome shotgun sequence.
ACCESSION   DASUFX010000641 DASUFX010000000
VERSION     DASUFX010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491011"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFV99_25905"
     CDS             complement(<1..1142)
                     /locus_tag="VFV99_25905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062845.1"
                     /translation="MENLDIDASVLQKIAMIERVSPEVARDIRDGLDDSIVEYARRMY
                     QLDRSPPSASVAFYRGKLAAKLASRAAELGVEVSLQRVLDSFAEFPIIQTAPHCQLYL
                     DPMAFWAYVVGWLGAFETGCQLIPVFNTATVTLQSAASSGPAWLSTATGALNITTSPS
                     SRLASTSVCAAGTAVAFDTAKLAALHGRAAPGEQRVIAMLERSIAASPRGFAHATDRT
                     NVNLLRSLSRARISPVMFSESLICDLMADLLADRDRLVWGLLFEPARNARLHAAFERA
                     STQLYGHFWSQATSHFWGIRDRKIRRLELCDGWLVEADRGDQDGLRVRFECDSLIEGL
                     RTELLVPNLFWSFALVTLLPRLSAIGGTRQIGYLNGFADVLRRALDL"
     gene            complement(1159..1878)
                     /locus_tag="VFV99_25910"
     CDS             complement(1159..1878)
                     /locus_tag="VFV99_25910"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5062846.1"
                     /translation="MITQRPVLGILGGMGPLSSAEFLCTLYECAADGRAEQDMPLALL
                     LSDSTFPPRTADSREESWAALRDRVEAGVQRLIAWGCDEIVLCCFTAHHVVPQLSPTS
                     AGRLRSLLDATVAELLAATRPCLVLCSTATRMLGLLETHPSWPAVRALVRFPDDADQE
                     RVNAMIKEVKSRGASAALVSELDLLVARYCVENAVFACTELHMLNRAFEKQRLATAYL
                     RRDPLYSLARAVCARTTPRQS"
     gene            complement(1875..3116)
                     /locus_tag="VFV99_25915"
     CDS             complement(1875..3116)
                     /locus_tag="VFV99_25915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5062847.1"
                     /translation="MLYDLTGALRRLRAVRREAWAIIGGLFVVGVGNYMVIPFFALYC
                     TKQLGLSAVEVGVLLTMRLWTQRGCALLGGMLADRFTPRRVMIAGMLCRFVGFLGIAA
                     SGQFYPLAIFTALTGFGSALYTPAGKAALIGLSAGGDKLLILALRNTAYNVGGAVGPV
                     VGLVGAFISYRWTLVGGGCLFVVAAIAIALLVTDRHAGHVARPMALRGARSLLADVRV
                     LVLCIALLVFFTFYVQLELTLPLFANHAFSDSAVALLFIVNAAVTILLQLPLSGWIDR
                     LRITSALAVGFGCTGLGLLLIAVPLGVPSFVLGMIVFSLGEILVEPKIDSELGGIVPG
                     SLLGTAFGLASLACSIGGAVGHELGAVGLPMAARLGGESWFFVALGIAGVIAAYAISM
                     TVRAVSANRFVHIPETTRSAA"
     gene            complement(3101..4327)
                     /locus_tag="VFV99_25920"
     CDS             complement(3101..4327)
                     /locus_tag="VFV99_25920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5062848.1"
                     /translation="MQALSSNDVGDVASSPANGEDTRASWFETFPAFTQGFLTWLSAK
                     PHVGQSASTHSPIGYLATSAATIAAGIGLFVVGASLDSWALRILAVAFTTAGLRKLQV
                     VVFHHCSHGTVFRGRLANTVLGESISAIVLIKSFAAYRRDHIRHHSSKHLLTAEDETV
                     QFLVSFARLEPGLSRRQLWWRMVLNFANPLFHLRWLSDRVISCFFVDGGPWRIVRVAY
                     WLALLSIVAWAGAWHGFVLGWLLPLTVCYNVSATLRLAAEHRWPTNSGVRSRAKDFVC
                     ETTVAVFLGAPLPRVVRWRRLYAVPLWLFHMVGHLLARVLVLVGDTPCHDYHHRRPSS
                     RAWPNAIFARQADLERGCPGYPQNYGEVWGLFRAIDEGFARLSTVDPHTLALASRRLP
                     ALSSRSRRKQVPCSTI"
     gene            complement(4578..4931)
                     /locus_tag="VFV99_25925"
     CDS             complement(4578..4931)
                     /locus_tag="VFV99_25925"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062849.1"
                     /translation="MEYSANLADAADLQRLLMSFHEALARIGVEIEDCKSRAYRCDVY
                     RVGGGARTRGFVHVTLAVLDHRPPEAQREAGELLLGILQRSVVSDLDCDFTVEVRPMR
                     TDGYFKVRAVAGPVA"
     gene            5438..>6754
                     /locus_tag="VFV99_25930"
     CDS             5438..>6754
                     /locus_tag="VFV99_25930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062850.1"
                     /translation="MITRRLGAGAMGTVYEARDPDLSRNVALKILRETGQHHSLRFLR
                     EAQTLAQLQHPNVVVIHDVGVTGDAVFIAMELVEGENLREYLAGNALSWSRVLAIYMQ
                     AGRGLAAAHEKSIVHRDFKPANVLIDRQGRVRVTDFGLARLVAEDTADADAAPSASFT
                     GAATAETVDNSADAHAATVDNSADVAHAATIDSSGDAAHAAGKGAGLLDSPLTYDGAL
                     LGTPRYMAPEQHARRRATARSDQFAFCVCVWEAVFGAHPFPHGQNSDTKPTRSERSAP
                     GWLVRALETGLAFDPARRHPTMDALLDALERAPRIRRRMWTAAGALAVVIAASLITLG
                     VRSTSDPCGGVAPLEAWTTEARVSTRNAFLATGLPFAGQIHDGVARRLDDYATRWHAM
                     KVDTCRADKSSSAAGAALSERRNLCLDRRQDEVGAMVAALRVITRDG"
BASE COUNT         1225 a         2275 c         2274 g          980 t
ORIGIN      
        1 aggtcgagcg cgcgacgcag cacgtccgcg aacccgttca gatagccgat ctgacgcgta
       61 ccgccgatcg ccgacagccg cggcagcagc gtgaccagcg caaacgacca gaacaggttc
      121 gggaccagaa gctcggtacg caagccctcg atcagcgagt cgcactcgaa gcggacgcga
      181 agcccgtcct gatcgccgcg gtcggcctcg acgagccatc cgtcgcacag ctccagcctc
      241 cggatcttgc gatcgcggat gccccagaag tggctggtcg cctgcgacca gaaatgaccg
      301 tagagctgcg tgctcgcacg ctcgaacgcc gcatgtagcc gagcattgcg cgcgggttcg
      361 aagagcaacc cccagacgag gcgatcgcgg tccgcgagca gatcggccat caggtcgcag
      421 atcagcgact ccgaaaacat cacgggagag atgcgcgcgc gcgacaggct gcggagcaga
      481 ttcacgttcg tccgatcggt cgcgtgcgcg aacccgcgcg gcgaggccgc gatgctgcgc
      541 tcgagcatcg cgatgacgcg ttgttcgccg ggggcggctc gcccatgcag cgccgcgagc
      601 ttggcggtgt cgaaggcgac ggccgtgccg gccgcacaca cgctcgtgct cgccagccgc
      661 gagctcggcg aggtcgtgat gttgagcgcg ccggtcgcgg tgctcaacca ggccggcccg
      721 ctcgaggccg cgctctgcag cgtcaccgtc gccgtgttga agaccgggat cagctggcag
      781 ccggtctcga acgcgccgag ccaacccacg acgtaggccc agaacgccat cggatcgaga
      841 tacagctgac agtgcggcgc cgtctggatg atcggaaact cggcgaacga gtcgagcacg
      901 cgctgtagcg acacctcgac gccgagctcc gcggcgcggc tcgcgagctt cgccgcaagc
      961 ttgccgcgat agaacgctac cgacgcgctt ggtggcgagc ggtcgagctg atacatccgt
     1021 cgcgcgtact cgacgatgct gtcgtcgagg ccatcgcgga tgtcgcgcgc gacctccggc
     1081 gacacccgct cgatcattgc gatcttctgc agcacacttg cgtcgatatc gaggttctcc
     1141 atgacgtcct cgtggcgatc agctctgccg cggcgtcgtg cgcgcgcata cggcgcgtgc
     1201 aagcgagtag agaggatcgc ggcgcaggta cgccgtcgcg agccgctgct tctcgaacgc
     1261 gcgattcagc atgtgcagct ccgtgcaggc gaacaccgcg ttctcgacgc agtagcgcgc
     1321 gacgagcaag tcgagctcgc tcacgagcgc cgcggaggcg ccgcgcgact tgacctcctt
     1381 gatcatggcg ttgacgcgct cctggtccgc gtcgtccgga aatctgacga gcgcgcgcac
     1441 ggcgggccag ctcgggtgcg tctcgagcaa gccgagcatc cgcgtcgccg tgctgcacag
     1501 aaccaggcac ggccgggtgg cagccaggag ctcggccact gtcgcgtcga ggagcgagcg
     1561 cagccgaccg gcgctcgtcg gcgacagctg ggggacgacg tgatgtgcgg tgaagcagca
     1621 cagcacgatc tcgtcgcagc cccatgcgat caggcgctgc actccggcct cgacgcggtc
     1681 gcggagcgcg gcccagctct cttctctcga gtccgcggtg cgcggcggga acgtcgaatc
     1741 cgacagcagc agcgccagcg gcatatcctg ttccgcacga ccgtcagcgg cgcattcgta
     1801 gagcgtgcac agaaactccg ccgaggacag cggccccatg ccgccgagga ttccgagaac
     1861 gggacgctgc gtgatcatgc tgcactccgt gtggtctccg ggatgtgcac gaagcggttc
     1921 gcggagaccg cgcggaccgt catcgagatc gcgtaggcgg cgatgacgcc ggcgatcccc
     1981 agtgcgacaa agaaccacga ctcaccgccg agtcgcgccg ccatcggcag gccgaccgcg
     2041 ccgagctcgt ggccgacggc gccgccgatc gagcacgcca ggctcgcgag cccgaaagcg
     2101 gtgccgagca gggagccagg cacgatccca ccgagctcgc tgtcgatctt gggctcgacc
     2161 aggatctcgc cgagactgaa cacgatcata cccagcacga acgacggcac gccgagcggg
     2221 accgcgatca gcagcaatcc aagcccggtg cacccgaagc cgacggcgag cgcgctcgtg
     2281 atgcggagcc ggtcgatcca gcccgacaac ggcagctgga gcaagatcgt gaccgccgcg
     2341 ttcacgatga acaacagcgc caccgcggag tcggagaacg cgtggttcgc gaacagcggc
     2401 agcgtcagct cgagctggac gtagaacgtg aagaatacga gcagcgcgat gcagagcacg
     2461 agcacgcgga cgtccgcgag cagcgaccgc gccccccgca gcgccatcgg ccgcgcgacg
     2521 tgaccggcgt ggcgatcggt caccagcagc gcgatcgcga tcgcagccac gacgaacagg
     2581 caccccccgc cgacgagcgt ccaccggtag gagatgaacg cgccgacgag tccgacgacc
     2641 ggtccgactg caccgccgac gttgtacgcc gtgttgcgca gcgcgaggat caacaacttg
     2701 tcgccgccgg cgctcaggcc gatcagcgcg gccttaccgg cgggcgtgta cagcgccgag
     2761 ccgaacccgg tcaacgccgt gaagatcgcc agcgggtaga actgtccgct cgcggcgatg
     2821 ccgaggaacc cgacgaagcg gcacagcatt ccggcgatca tcacgcgccg aggcgtgaag
     2881 cgatcggcga gcatgccacc gaggagcgcg cagccccgct gggtccacag ccgcatcgtc
     2941 agcaacacgc cgacctccac cgcgctcaac ccgagttgct tcgtgcagta cagcgcgaag
     3001 aacgggatga ccatgtagtt gccgacgccg accacgaaca gcccgccgat gatggcccac
     3061 gcctctctcc ggaccgcgcg aaggcgacgg agcgcgcccg tcagatcgta gagcatggca
     3121 cctgcttcct gcgcgacctc gacgacagcg ccggcagccg acgcgacgcg agcgcgagcg
     3181 tgtgcgggtc gaccgtgctc agccgcgcaa agccctcatc gatcgcgcgg aacaagcccc
     3241 agacctcgcc gtagttctgc gggtagcccg gacagccgcg ctcgaggtcc gcctggcgcg
     3301 cgaagatcgc gttgggccac gcgcgcgagg acggccgccg gtgatgataa tcgtggcacg
     3361 gcgtgtctcc gacgagcacg agcacgcgtg cgagcaggtg gccgaccatg tggaacagcc
     3421 agagcggaac cgcatagagc ctccgccacc tgaccacgcg cggcagcggc gcgccgagga
     3481 acaccgccac ggtcgtctcg cagacgaagt ccttcgcgcg cgatcgcacg ccactgttcg
     3541 ttggccaccg atgctcggca gcgagtcgca gcgtggcgct cacgttgtag cagaccgtca
     3601 gcggcaacag ccaaccaagc acgaacccgt gccaggcccc ggcccacgcg acgatcgaca
     3661 gcagcgccaa ccagtacgcc acgcggacga tgcgccacgg cccgccgtcg acgaagaagc
     3721 acgagatcac gcggtcgctg agccagcgca gatgaaacag tgggttcgcg aagttcagaa
     3781 ccattcgcca ccacaactgg cgccggctca gcccgggctc gagtcgcgcg aagctgacga
     3841 ggaactgaac ggtctcgtcc tcggcggtca gcaggtgctt gctgctgtga tgccggatgt
     3901 gatcacgcct gtacgccgcg aatgacttga tgaggacgat cgcggagatc gattccccga
     3961 gcaccgtatt cgcgaggcga ccgcggaata ccgtgccatg cgagcagtga tgaaacacga
     4021 cgacctgcag cttgcgcagc ccggccgtgg tgaacgcgac cgcgaggatg cgcagagccc
     4081 acgagtcgag gctcgcgccg acaacgaaca gcccgatccc agcggcgatc gtcgcggcgg
     4141 aggtggcgag gtaaccgatc ggagaatggg tcgaagccga ctgcccgacg tgcggcttgg
     4201 cagataacca ggtcaagaac ccttgcgtga atgcagggaa cgtctcgaac cacgaggcgc
     4261 gcgtgtcctc accattcgcc ggcgacgacg cgacgtcgcc cacatcatta ctcgacaacg
     4321 cctgcatggc actccgttac gcaacgcggt gcccggtgat gttgtcgccg cgggcacgcg
     4381 gttcgcgcac acttagcaac gcgcgcgcca agctgaccgc tcacgaatca gcgaaagaaa
     4441 cgggtccgta cgcgagcgtt tcgccgtgtt cggttcgtgt ggctgttcct ggtgttccga
     4501 tccgcggcgc gccgtcgcga tccgtgattc ggatgttccg ctcggcggta ccggtgtgtt
     4561 ccggccgctc gacggcgtca ggcaaccgga ccggcgaccg cgcgcacttt gaagtagccg
     4621 tccgtccgca tcgggcggac ctcgacggtg aagtcacagt cgaggtcgct cacgacgctg
     4681 cgctggagga tcccgagcag cagctccccg gcctcgcgct gagcctctgg cggacggtga
     4741 tcgagcaccg cgagcgtgac atggacgaac ccgcgcgtgc gggcacctcc gcccacgcga
     4801 tagacgtcac atcggtacgc gcgactcttg cagtcctcga tctcgacacc gatccgcgcg
     4861 agcgcctcgt ggaaggacat gagcaggcgc tgcagatcgg cagcgtcggc gaggttggcc
     4921 gaatattcca cggtgatgtg cggcatgggc gcgtggcatc gtcgctgatc ggctgtggtc
     4981 gcgctaccgg cgtgactcgt tctgacggga tccgcttcac acgtcgatcg cgtggggcat
     5041 ggcgaagtcc acgtaagcat cgtgctctct ctgttcacgg cgcatcgagt ttgttcacga
     5101 gcacgtcgag aagcttcgca atgttctcca gcgacatcac cgttccgcaa actgtggtcg
     5161 gaaatccacg acgttgcatg agttgcggag cgattggccg cgcaacctcg cgacaagatt
     5221 gagcgcctct ttcatcgcac tctagaggtc aggggttcga tccccctcgg ctccaccagg
     5281 aaacagaacc gccgcgtaga gatacgcggc gtttttgttt tcggtcgaag tgttcgcaag
     5341 ggtagtttct tcgcaagtga gcggagtgcc gttacgtttg ccgtcgggaa gcaccgacgg
     5401 ggatgcgctg gcacctgaga gccgggtggg gaggttcgtg atcacccgac ggctcggcgc
     5461 cggcgccatg ggcaccgtgt acgaggcgcg cgatccggac ctgagccgga atgtggcgct
     5521 gaagatcctg cgcgagaccg ggcaacacca ttcgctgcgg ttcttgcgcg aagcacagac
     5581 gctcgcgcag ctgcagcatc ccaacgtggt cgtcattcac gacgtcgggg tgacggggga
     5641 cgcagtcttc atcgcgatgg agctcgtcga aggtgaaaac ctgcgcgagt acctcgccgg
     5701 caacgctctg tcctggagtc gtgtgctcgc catctacatg caggcgggga gagggctcgc
     5761 ggccgcgcac gagaaatcga tcgtgcaccg tgacttcaag cccgcgaacg tgctgatcga
     5821 tcgtcagggt cgcgtgcgcg tcaccgactt cggcctcgcg cgtctcgttg cggaggacac
     5881 cgcagacgcc gatgcggccc cgagtgcgtc gttcaccggg gcagcgactg cggagactgt
     5941 cgataactcg gccgacgcgc acgccgcgac tgtcgacaac tcggccgacg tcgcgcacgc
     6001 cgcaactatc gacagctcag gcgacgccgc gcacgccgcg ggcaagggcg ccgggttgct
     6061 tgactcgccg ctcacgtatg acggcgcgtt gctcgggact ccgcgataca tggcaccgga
     6121 gcagcacgcg cgtcgacgtg cgaccgcgcg gagcgatcag tttgcgttct gcgtatgtgt
     6181 ctgggaggcg gtatttggcg cgcacccgtt tccgcacgga caaaattccg acacgaagcc
     6241 gacgaggtcc gagcgctcgg cgcccgggtg gctagtgcgc gcgctcgaaa cgggccttgc
     6301 attcgatccg gcgcggcgtc atccgacgat ggatgcgctg ctcgatgcgc tcgagcgtgc
     6361 cccccggatt cgccgtcgga tgtggacagc cgcgggcgcg ctcgccgtcg tgatcgccgc
     6421 atccttgatc acgctcggtg tgaggtcgac gtcggatcca tgtggcggcg tggcgccgct
     6481 cgaggcctgg acgaccgaag cgcgggtatc gacgcgcaac gcgttcctcg cgacggggct
     6541 gccgtttgcc ggccagatcc acgacggcgt cgcacgtcgc ctcgacgatt acgctacccg
     6601 gtggcacgcg atgaaggtgg acacctgccg cgccgacaag tcgtcgtccg cagcgggcgc
     6661 agcgctctcc gagcggcgca acctgtgcct cgatcggcga caagacgaag tgggggcaat
     6721 ggtcgccgcg ctgcgcgtga tcacacgcga cggc
//
LOCUS       DASUFX010000642        22606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1353048, whole genome shotgun sequence.
ACCESSION   DASUFX010000642 DASUFX010000000
VERSION     DASUFX010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353048"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFV99_25935"
     CDS             complement(<1..621)
                     /locus_tag="VFV99_25935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062851.1"
                     /translation="MVAPAKSASVKRLTGSKVGKYTLKELLGQGGFGEVYVGEQKGGP
                     NVAVKILDAAHAKDDDAIERFKREGETAKRLDHPNIVRVLDVGSSRAKHYLVMELVAG
                     GSLERLMRRDGVAEKVLPALVASAEALAYAHGQGVVHRDVKPANILLTKAGRAKVTDF
                     GLARTVDHSSMTTEGRLLGTASYMSPEQVKGERASAASDVYAMGVMI"
     gene            complement(678..1763)
                     /locus_tag="VFV99_25940"
     CDS             complement(678..1763)
                     /locus_tag="VFV99_25940"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5062852.1"
                     /translation="MNRAAELLAELVAIPTQQAGPDGKAGDERRLCDHLAPLLRARGA
                     DEVIVADAKRTDGSPGAYVFARWGTPRRIINAHVDTVPANAGWSRDPWKPHIENGRLY
                     GLGSADTKGAIAATLVALDAVKPTNAGVLFSGDEEHGSRVMHAFLQSKYAKDVKQAIV
                     CEPTARTAGIRHRGVIGQTATLEGEGGHSSKADYMPKPLLQLARLAVAINGLGLARLH
                     DGPAGMTGICLNIAALHGGVAFNVIPQRGQLEWSLRPYPGFDRAKWDEDIAAAMHAVD
                     PKIVLAGSVDHAPFQCDKLADAVKPFVEKIGGLDFWTEAALYEERGIDAIVVGPGDIG
                     HAHAADEYVTLEDLDWAVELYRSLLAG"
     gene            complement(1828..2460)
                     /locus_tag="VFV99_25945"
     CDS             complement(1828..2460)
                     /locus_tag="VFV99_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5062853.1"
                     /translation="MSTLVQLRRHVAQLRECAACPDMIGPVVTGKPVMSPVMLIGQAP
                     GVKEGPAGKPFAWTAGKTMFGWFATLGLDEEEFRSRVYMAAVCRCFPGKAPGGTGDRV
                     PSPDEVERCSKHLHAEIKILRPRLIIPVGKLAIAQLYPDIDKLTEVIGEKRSGELAGH
                     TFDVIALPHPSGASTWHRTEPGKTLLAQALARIGKHEAWRALTGAEAATA"
     gene            2585..3175
                     /locus_tag="VFV99_25950"
     CDS             2585..3175
                     /locus_tag="VFV99_25950"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX5062854.1"
                     /translation="MNTLEHEPLIKRVLGTNYLQLGLAKLLIEEDPQKGATVKVTVNE
                     GDTPIEVEGKGVGVVDALYSGLLGRYAREYQSLKTIELASFAVAADVETKKAQAGVDA
                     VGRVTLDVRNSEGRHFTFSHASRSVMTSTACAVIACVQYFVNAERAFLTLHNARRDAL
                     ARGREDLVARYTAEMAEVVEATSYADVIANIRKELA"
     gene            3201..6080
                     /locus_tag="VFV99_25955"
     CDS             3201..6080
                     /locus_tag="VFV99_25955"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062855.1"
                     /translation="MQPGTVVAGKYRVERIIGRGGMGLVVEATHLGLGHRVALKFLAE
                     GMASDQTALARFRREGQAAAALKSEHICRVFDVGVDGKTPFIVMEMLNGTDLARLSKV
                     RALDVPTAALYVRQACLGLAEAHAAKIIHRDLKPGNLFITRRADGSALIKVLDFGVAK
                     APEESDIKLTGTSNVVGSPGFMSPEQFRSSKTVDGRTDVWSLGVILYKLVSGRLPFKA
                     DSFAEFALAITRDPVPPLPEASPEFEAVIGKCLEKDPAKRYPDVLALAEALAPFAAQA
                     RGSAPTIPVDAPASDEVMSGVGSLPDSATHTLLGTPQRLPAQPAKPQVTAPAKPAGVA
                     AAKATMLGVSQAGVKPIVQSPDPVKLPLPAPPPTVAPGVSPLMKPTAAETAVPSPGTM
                     PGTVAPRFVPVGELPPGTVVGEYKIDAKIGEGGMGIVYSATHPLIGKRAAIKVISAEL
                     GTDPVLVQRFVQEARSVNQIGHPNIVDVFAFGKLPDNRNYFVMEYLQGESLRARLTRT
                     FMSTSDAIQILDEIAGALEAAHEKGIVHRDLKPDNVYLASVRGNFIIVKLLDFGIAKL
                     VTEGGIAKTSTGEMMGTPGYLSPEQARGKNVDYRTDVYALGCMMYEMITGRVPFQGES
                     PMDIVMMHITTPPEKPSTYKPDIPPLLEQVILEMLDKDPQKRPSLGHLRNVFAELVAS
                     GLVLLEPGSASTFRSDLMRRRGDTENRTPRGTGSNARAQTPTPAPPVAVPTGQPNQHT
                     LGRRRNASPSDAPTQIAFTPGPNTITPANEPKTAALPAQPAPRKSRTGLIIAIVLVAA
                     AVPAVTIALGLMKQHDSAPADAAAHVVATASDGAVAAVVADAPAADAAVAAVTVDAAV
                     AARDVIIRINVPSAKVEIDGAPVLDDKGVVRVQLTDGPHRIAASASGRTPIDKTVDVS
                     ASANAFSLVLERAKSNTKQHPAGQGSGSGQGSAVQKDYTVDPFKE"
     gene            complement(6117..6977)
                     /locus_tag="VFV99_25960"
     CDS             complement(6117..6977)
                     /locus_tag="VFV99_25960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062856.1"
                     /translation="MTKNLVLAMIVASATSALAQPAPPTPPPQGTPATADKEAEAKAH
                     YDKGISHYNLGEFDPAITEFKAAYAISSKPGLLFNVAQSYRLKKDYEQALYFYQTYLR
                     LKPDAPNRQDVEDRMKEMQDAIEEQKKIGEKKPIGTMTPEGGTTTTTTTAPNGTTTTT
                     TTTAGPTTTDDGGVKAQSLLTAGYVTGGAGAALLITGVIFGSMARSAEKDLNNLSSDH
                     GTWTPEQQDKYDTGKRNNTIAIVSFIAGGAALATGATLFVLGTMKKSKTTVAINPTAH
                     GTTFAVGWSF"
     gene            complement(6990..8795)
                     /locus_tag="VFV99_25965"
     CDS             complement(6990..8795)
                     /locus_tag="VFV99_25965"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062857.1"
                     /translation="MTACPDDGWSLTVEMDATYAPPTGGMAAVAAPASAAMAVAHAPV
                     EEAPPTTDLIPGTVVGEYKVDKKIGEGGMGAVYSATHPMIGKRAAIKVISAALGTDAS
                     AVGRFVQEARSVNQIGHPNIVDVFAFGELPDGRNYFVMEYLQGESLADRLQRAEMPLG
                     EAIEILDQVADALEAAHEKQIVHRDLKPDNVYLASVRGGRTMVKLLDFGIAKLSTPDG
                     PGSGGGIAKTRTGMMMGTPGYLSPEQARGKNVDYRTDIYALGCMVFEIVCGRLPFISD
                     NAMDIILMHMTTPPVRASSVWPEIPAPLDDLIARMLEKEPSARPSLPEVRAVFAELVT
                     SGLVTLNNGRGIAFGSNISSRVNTPAGGTPRPGTMNLAVGTKPATVAPSTHPPGTQPP
                     MQVAEAPKKKTGLIVGIAAAVVLLGGAGAFFALKGGGGGEKPKEAPVATTEPAKPPEP
                     ATTPEPAKTPEPAKAPEPVKVEAPKPEPGTINIVISVDKAQVSVDGNPVEVKGKTAKA
                     TVETEGSHLVQVTAAGREPFEQTVDTKAGGSVQIVAKLERSKSSSTKKATVGTKTNTT
                     QTKTTPTGTGTGSAATTTTTTTTKTTDKNETINPF"
     gene            complement(8862..13532)
                     /locus_tag="VFV99_25970"
     CDS             complement(8862..13532)
                     /locus_tag="VFV99_25970"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEX5062858.1"
                     /translation="MCGPNGECPPGLDCYGGVCRADNPGIDASNTFPLTISLGGNSMG
                     TVTSNPAGINCGTDCTESFTSGTMVTLTATPTTGSLFVGWSGACSGTGACTITISTAV
                     TVTANFALDNSLVVTLGGNGSGLVTSNPAGINCGTNCSYQFPNNTMVTLTAFAGTGST
                     FDGWTGAGCSGTGTCTVTTDMATMVTATFSLTQLSLTVVNAGNGQGTVTSNPTGISCG
                     VDCTDAYNYNTMVTLTAAPSTGSTFTGWSGGGCTGTGTCTVTVTAATTVTATFTLIQH
                     SLTVQKSGTGGGTVTSTPAGINCGTTCTQMFDYGTMVTLSAAPATGSTFAGWSGGGCA
                     GTSSCTVTMTMANTVTAIFTLSNYNLTVSKAGNGSGTVTSTPAGISCGTDCTESLTFG
                     TMVMLTAAPATGSTFTGWSGACTGASTTCMVTIGAATDVTATFALNQYLVTVMKSGTG
                     NGTISGVGFMCGADCTENVDYGTIVTLNANPSAGSTFDGWTGGGCSGTGSCQTTITAA
                     LTVTAQFTLSQYSLSVMKAGTGAGTVTSTMPSGGITCGADCTELYDYGTMVTLSAAPQ
                     AGSSLTSWSGGGCTGSAMTCTVPVTAATTVTATFTLSTPTLTVATNGGSGTGNVTASP
                     PGINCGNGGSDCTEPYPYGTMVTLSAVPSGTSFFVGWSAASCPGNGSCTVTMDAAKTV
                     TATFDLGSQSLSVTKLGNGSGTVTSNPAGISCGATCSFNFTNMTMVTLTATAATGSTF
                     TGWSGGGCSGTSTCTVTIGAAVNISATFQLNVHMLTVMKAGNGATGSTVTQTSPPPPQ
                     QQINCGGTCNANFDYGTAVTLSATPAANAVFTGWSGGGCSGTGTCVTTLTANTTVTAN
                     FSLVQRSLSVRPIGNGLGTISSSPAGINCGADCDELYNDGQVVMLTATPDPGYEFTGW
                     AGDGAACGTMTTCSVTMNADKGVYADFEPQQYLLSVTKNNNNAGDVFSTTDGGIDCGT
                     KSCSALYDYKTFVSLKASERSGFTFTGWSGDCTGNGSCDVTMDQAKNVTANFVVTTWN
                     LAVTKQGNGAANGTVTSTPGGISCGATCSSTYNDGTQVTLNATATTGTNFTSWSGAPG
                     CSSNPSCVVTMNQAYNIIANFQLQTFSLNLNKGSSTGTGTVTSSPGIINCGPTCTSAS
                     QSFNYNTMVTLTATAAYGSTWGGWSGSVPANCTPAPPAQPPTTCTVTMTAAQTVTATF
                     NAVPANRVFVTNATYALNTIGGTSGADTICQNIANGFTPPLGGAGSTWVALLSSSSSD
                     AATRIGSASGWVRTDGRPFANTKADLLNGRWFTPARFNQAGADLGDVEYVTGTTTNGV
                     YSGQGDCTNYSTTTGNVTKGEAQATSVSGYNFGTVGCSAMTMHLLCVQTNRQAVVTVA
                     APTGTYRRAFMRLWASGGGIADADAKCQMDANNHVGGALPGTYKALLATTSATAISRF
                     NTSTSLGTWYRLDNIALVPTPTALGTATYFDTPPAMFIDGSYQSNWANWAGAPNFTTV
                     SAAGGGDNCTNYTETSAASMHARTGNANMSRVSGASPSFLGPNNPSYKDCGFTSISIL
                     CLQE"
     gene            13775..14578
                     /locus_tag="VFV99_25975"
     CDS             13775..14578
                     /locus_tag="VFV99_25975"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5062859.1"
                     /translation="MIVPFLVCGRQSGPALRVATFNIEDFPKNRRQIDGAFAEMAALG
                     AGIIAVQEIGEPGLFAHEAHRLLGERWGFAHVDTRPIGETRRGHHIGVLFDGARWQRI
                     GMRVHDATRLEGGRHKPTLEVRLRSGDLVVHVLVVHLKSGSDGRDIRARQFAALAGIV
                     REVQRAGEPIVLAGDFNATELGDRDDLDRLARDTGLVWASEPLACSAFWARDDGCPRS
                     RLDHMLTWKKPQSIRAAGACATEGCDWQASCPVYTQHVSDHCPVVVTFE"
     gene            14624..15058
                     /locus_tag="VFV99_25980"
     CDS             14624..15058
                     /locus_tag="VFV99_25980"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5062860.1"
                     /translation="MRFAVGLVCVAACATRQAPPPTNVEHAQPAKVRPAKVRTAKAAN
                     SNAPRTHAELTPDVVLATIRSRYIGGVERCYRRHLKNDATARGRVLVSFTVDPKGRAR
                     DGEAHGITERVDDCIAAQVARWQFPKPGQESRFALGLELGVD"
     gene            complement(15275..18076)
                     /locus_tag="VFV99_25985"
     CDS             complement(15275..18076)
                     /locus_tag="VFV99_25985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062861.1"
                     /translation="MRDLVDELLHAKVKAKLFGGEHAPKLGRLVLVDRIGAGAMGTVF
                     AAYDPRLERKVAVKVLRSSEEAARVLGEARALAKLAHPNVVTIYDADELDGLVYIVME
                     LAPGVSLRTWIGAHRDWRDIVRVMREAGAGIAAAHAAGLVHRDIKPDNILVGDDRTRV
                     VDFGLAAPRADTEGESAGTPFYMAPEVLDGGAATEASDQFSFGVTLYEALYGRRPHGP
                     LAPTASGEAATIPASMATKTHRDLIANLREVATQASTARPPSSSVPAWLHAIVARALA
                     PRPAGRFASMSELVTALGRDRRRSRTIVLGATALAMSGILGAAIYRTQSRGESCTGGP
                     ARSETVWNDTQRTKVRDALGKTRWAPQTVAALDARAEQWQLSYRRVCEATRHGEQSDA
                     LLDLRMRCLDRVLDHFGALTGALAGSALEPSARVAAPGAIDELPSIEDCERLSDPAEL
                     ALPTDRKQREQVTAVEHDLARASAAFALGRYNEARGIVGEADGKLAGITAPRVRAESL
                     LLSSSIEARIGDGAVARSKLDEALVAAADAKAPELELDVWSRRLRNELFAGDPAKVIE
                     QAPFARAAAKRANRQGADIDGVVAQALRNAGQLGEARELLDRALASTDPLRENQRSLL
                     EMNRGSIELAVGNSAGALTLFQRAYDRVLSALGDNHPELAIYIDKLAAAKRSRGKLRE
                     ALQLHDRSIELRIAAFGTEDRAVATGLLYRAQTKLEAGDIEGARRDLDDARAIRQKTF
                     GATSPRVGEIVAAQGELASAAGNQERALILYDEAIKLDPRLELAARRFAAGGTVDLDA
                     IAPLGPGELLSIERAGALAARIALLQNANRADEARALAATLRARYKPGEDPALASALA
                     SAQLAAGDRTAAADILAKTAAALGNEPTRTALHVFTQLAHASDTQAQAAARAAIALYQ
                     AMPALDRAEHDEMWAISRR"
     gene            18099..18674
                     /locus_tag="VFV99_25990"
     CDS             18099..18674
                     /locus_tag="VFV99_25990"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5062862.1"
                     /translation="MRSDGELLTAWRAGDAAAGEELVARHWTSLSRFCRSKIGDDGAD
                     VIAQVFLACVEGRDRIAGDNVKAYLFAVARKRLAEHFRQTARRADLDLDVSSVADLRT
                     GPATHVARRQRSELLREALTRIPLDDQIAIELAYFEGLAMKEIADVLEIAENTVRSRL
                     SRAREKLRAVLATLGSADEAALAESQLDGSS"
     gene            18801..19382
                     /locus_tag="VFV99_25995"
     CDS             18801..19382
                     /locus_tag="VFV99_25995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062863.1"
                     /translation="MRVLLIAIALLVPARAVADDDEVRHDERPTAWLFRATPGFGYSW
                     DRDVMCAVQGRMMFGGWFVGKVVTPSLVVGGALRMDVNALPDDGCVTDDDGLRMTIGM
                     VLGPEIGWYPFDAGLHVFAGGGFATFDHDSMYAAYGVGGTLAVGYDWAFRKEGATGAF
                     RFGLAAQVTAVRMSHGHDMTMPAGVMTIGTEWR"
     gene            complement(19379..20812)
                     /locus_tag="VFV99_26000"
     CDS             complement(19379..20812)
                     /locus_tag="VFV99_26000"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5062864.1"
                     /translation="MHLSDLDATAQAELVRRGDASPRELVDAAIARIEKHNPALGAVI
                     IPLFEQARATADDAPHGPFRGVPILIKDILATVGGVLYTAGLKPLREANYTSPVDSYL
                     VSQLRKAGFVIVGKTNTSEFGIVPSAEPPAWPPARNPYDTTRSTGGSSGGSAAAVSSG
                     MVPVAHANDGVGSIRIPASCCGLFGLKPSRGRVSFAPNYGDVNNGLVNEHVVARSVRD
                     SAAILDLLAGVQPGDPYTAPPQLGPYAAEVSVPPGKLKIGFATRRITTEGTFVDSHPD
                     CIAAVQHTAKLLESLGHHVEHAEIAATSDPGWVPNFLAAWAVGVTHDLDSASEKLGRT
                     IRMHEIEPLTWGLAELGRLVSAASYVKAWDWLRAASRRIATFFERYDLWLTPTVAEPP
                     PPLGTFKSPPDDILAGIFRAGDFAPFTLLFNTTGQPACSVPLYRNAANLPIGVQLVAA
                     YGREDLLFRVAAQLEAAQPFDHAATRR"
     gene            20847..21602
                     /locus_tag="VFV99_26005"
     CDS             20847..21602
                     /locus_tag="VFV99_26005"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5062865.1"
                     /translation="MQHLAGKLALVTGGGRGIGAACAHGLAAAGAKVIVCGRSQPDLE
                     RVAKEIGGTAIVADLADRAATDRMLLELPGRIDVLVNNAGVAESAALDRTNDELWDRI
                     LEIDATAPFRVIRALVPAMVKAGWGRVINIASNAGVSGYGYSSAYCAAKHAMVGFTRA
                     LAIDLARTGVTINALCPGWVHTKMSDEAVARIAAKTGRSADEARASLEAMSPQRRMIE
                     PEEVAHATVMLCAEASRGIHGQTIVIDGGAILK"
     gene            21599..22003
                     /locus_tag="VFV99_26010"
     CDS             21599..22003
                     /locus_tag="VFV99_26010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5062866.1"
                     /translation="MSTEGVVVPGWPAPKGYSNGRVGRGPVLHVAGQIGWTPDGTFEA
                     KDLVGQFAVALANVIEVVHAAHGKVTDIAEMTVYVTDIAAYRAARKQLGPIWKERMQS
                     HFPAMALVAVSALVEPEALVEIQAVAYLEHPE"
     gene            22000..>22606
                     /locus_tag="VFV99_26015"
     CDS             22000..>22606
                     /locus_tag="VFV99_26015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5062867.1"
                     /translation="MIPTPKSFALELSRGVLTITLDRPKRLNALTFEVYQELAETFEK
                     LDAYEEVRAIVITGRGRAFCSGGDQDDIIKHLLGRDTPALLAFTRTTGRLIAAIRRCR
                     RPVIAAINGVAVGAGAVIACAADLRIAASAAKFGFIFPPVGLSGADMGITYLLPRIVG
                     LGHASELLFFGDIIDAETAHRIGLVNRVVPDGAAALAMAREW"
BASE COUNT         3520 a         7349 c         7789 g         3948 t
ORIGIN      
        1 gatcatcacg cccatcgcgt acacgtccga cgccgcgctg gcgcgctcgc ccttgacctg
       61 ctcgggcgac atgtacgacg cggtcccgag cagacggccc tccgtcgtca tcgacgagtg
      121 atcgacggtg cgcgcgagcc cgaagtcggt gaccttcgcg cggcccgcct tcgtcagcag
      181 gatgttcgcc ggcttgacgt cgcggtgcac gacgccctgc ccgtgcgcgt acgcgagcgc
      241 ctcggcggac gcgacgagcg cgggcagcac cttctcggcg accccgtcgc gccgcatcag
      301 gcgctcgagc gagccgccgg cgacgagctc catcacgagg tagtgcttcg cccgcgacga
      361 gccgacgtcg agcacgcgca cgatgttcgg atggtcgagc cgcttggcgg tctcgccctc
      421 gcgcttgaag cgctcgatcg cgtcgtcgtc cttggcatgc gctgcgtcga ggattttcac
      481 cgcgacgttc gggccgccct tttgttcgcc gacgtaaacc tcgccgaagc cgccctggcc
      541 gagcagctcc ttgagcgtgt acttgcccac cttcgagcca gtcagccgtt tgacggaggc
      601 ggacttcgcg ggagcgacca cggcaacgtc atacacccaa gcggcgacgc gtgggcggga
      661 gcgccgctaa cgagccgcta accagcgagc aacgaacgat agagctcgac cgcccagtcg
      721 agatcttcga gcgtgacgta ttcgtcggca gcgtgcgcgt gaccgatgtc gccagggccg
      781 acaacgatcg cgtcgatgcc gcgttcttcg tagagcgccg cttcggtcca gaagtcgagc
      841 ccgccgatct tttcgacaaa cggtttcacc gcgtcggcga gcttgtcgca ttgaaacggc
      901 gcgtgatcga ccgaacccgc gagcacgatc ttcggatcga ccgcgtgcat cgctgcggcg
      961 atgtcctcgt cccacttcgc gcgatcgaag ccgggatacg ggcgcagcga ccactcgagc
     1021 tgaccgcgct gcgggatcac gttgaacgcg acgccgccgt gaagcgcggc gatgttgaga
     1081 cagatgccgg tcatgcccgc cgggccgtcg tgcaggcgcg cgagcccgag cccgttgatc
     1141 gcgacggcaa gccgcgcgag ctggagcagc ggctttggca tgtagtccgc cttcgacgag
     1201 tgaccgcctt cgccctcgag cgttgcggtc tggccgatca cgccgcgatg ccggatgcca
     1261 gccgtgcgcg ccgtcggttc gcacacgatc gcttgcttga cgtccttggc atacttcgac
     1321 tgaaggaacg cgtgcatcac gcggctaccg tgctcttcgt cgcccgagaa cagcacgccc
     1381 gcatttgtcg gcttcaccgc gtcgagcgcg acgagtgtcg ccgcgatcgc gcccttggta
     1441 tcggcggagc cgaggccgta cagcctgccg ttctcgatat gcggtttcca cggatcgcgc
     1501 gaccagcctg cgttcgcggg gacggtgtcg acgtgcgcgt tgatgatccg ccgcggcgtg
     1561 ccccaacgcg cgaatacgta ggcaccgggg cttccatcgg tgcgcttcgc gtcggcgaca
     1621 atcacttcgt ctgcgccgcg cgcacgtaac agtggagcaa gatgatcgca gagccggcgc
     1681 tcgtcgccgg ccttgccatc gggtccggct tgttgcgtcg ggatcgcgac gagctcggcg
     1741 agcagttcag cggcgcggtt cacgcgcgca gcctatcact cacgcctgca cgacggcggc
     1801 tcaactacgc agtcgcggct tcggcggcta cgcagtcgcg gcttcggcgc cggtgagcgc
     1861 gcgccacgct tcgtgcttgc cgatcctcgc gagcgcttgt gcgagcaacg tcttgcccgg
     1921 ctcggtgcga tgccacgtcg atgcacccga cggatgcggc aacgcgatca cgtcgaacgt
     1981 gtgaccggcg agctcgccgc tgcgcttctc tccgatcacc tcggtgagct tgtcgatgtc
     2041 ggggtagagt tgtgcgatcg cgagcttgcc gacaggaatg atgaggcgcg ggcgcaggat
     2101 cttgatctcg gcatgcaggt gcttgctgca gcgttcgacc tcgtcaggcg acggtacgcg
     2161 gtcaccggta ccaccaggtg ccttgccggg aaaacaacga cacaccgcag ccatgtaaac
     2221 gcgactacga aattcttctt cgtcgagacc gagggtggca aaccacccga acatggtttt
     2281 ccctgcagtc cacgcgaacg gtttaccggc aggaccttcc ttcacacctg gcgcttgccc
     2341 gatcaacatc acaggcgaca tcacaggttt gccggtcacg accggaccga tcatgtcagg
     2401 acatgcggcg cattctctga gttgcgcgac gtgtcggcgg agctggacta gcgtggacat
     2461 cgagcgcgac cactatggcg tacgaccctg acgctggcac gcgcgggagt gggtacaagt
     2521 gcctggagat gccactgacc tagatcacgg gcagggttgc ggttcacacg gtaagatcgt
     2581 cgacgtgaat actctcgaac acgagcctct gatcaagagg gtcctcggta cgaactatct
     2641 gcaactcggc ctggcgaagt tgctcatcga agaggatccg cagaagggcg cgaccgttaa
     2701 ggtgaccgtg aacgagggcg atacgccgat cgaggtagag ggcaagggcg ttggcgtggt
     2761 cgacgcgctc tacagcggat tacttggtcg gtacgcgcgc gagtaccaat cgctcaagac
     2821 gatcgagctt gccagcttcg cggtggcggc agatgtcgag accaagaagg cgcaagccgg
     2881 tgtggacgcc gttggtcgcg tgactctcga cgtgcgcaac tccgagggcc ggcacttcac
     2941 gttcagccac gcatcgcgct cggtgatgac gtcgaccgcg tgtgccgtga ttgcgtgcgt
     3001 acagtacttc gtcaatgccg aacgcgcgtt cttgacgctg cacaacgcgc gacgcgatgc
     3061 acttgcccgc ggtcgcgaag atctcgtcgc gcggtacacg gccgagatgg cggaggtcgt
     3121 cgaggcgacg agctacgccg acgtgatcgc caacatccgc aaagagttgg cttagcgatc
     3181 ggtgatacga tggcccgggt atgcagccgg gcacggtcgt cgcggggaaa taccgcgtcg
     3241 aacgcattat cggtcgcggc ggcatggggc tcgtcgtcga agcgacgcat ctcgggctcg
     3301 gacaccgcgt cgcgctgaag ttcctcgctg aagggatggc gagcgaccag accgcgctcg
     3361 cacggtttcg gcgcgaaggg caggccgcgg cggcactcaa gagcgagcac atctgccgcg
     3421 tgttcgacgt cggggtcgat ggcaagacgc cgttcatcgt catggagatg ctcaacggca
     3481 ccgacctcgc gcgactttcg aaggtgcgtg cgctcgacgt cccgaccgcg gcgctctacg
     3541 tccgccaggc gtgcctgggc ttggccgagg cgcatgccgc gaagatcatc caccgcgacc
     3601 tcaagcccgg gaacttgttc atcacgaggc gcgcggacgg cagcgcgctg atcaaggtcc
     3661 tcgacttcgg cgtcgcgaag gcgcccgagg agagcgacat caagctgacc ggcacgtcga
     3721 acgtcgtcgg ctcgccgggc ttcatgtcgc ccgagcaatt tcgctcgagc aagaccgtcg
     3781 acggcaggac cgacgtgtgg tcgctcggcg tgatcctcta caagctggtg tcgggccggc
     3841 tgccgttcaa ggcagacagc tttgccgagt tcgcgctcgc gatcacgcgc gatccggtgc
     3901 cgccgctgcc cgaggcgtcg cccgagttcg aggccgtgat cgggaagtgc ctcgagaagg
     3961 atccggcgaa gcgctatccc gacgtgctcg cgctcgccga ggcactcgcg ccatttgcgg
     4021 cgcaggcgcg tggctcggcg ccgacgattc cggtcgacgc accggcgagc gacgaggtga
     4081 tgtcgggtgt cggaagcttg cccgacagcg cgacgcacac gctgctcggc acgccgcagc
     4141 gcttgccggc ccagcccgcg aaaccgcagg tgaccgcgcc ggccaaaccg gcgggtgttg
     4201 ccgcagcgaa ggcgacgatg ctcggcgtgt cgcaagctgg cgtgaagccg atcgtgcagt
     4261 cgccggatcc cgtgaagctg ccgctgccag cgccaccgcc gacggtcgcg ccgggtgtat
     4321 cgccgctgat gaaaccgacg gcagccgaga ccgcggtgcc gagtcctggc acgatgccgg
     4381 gaactgtcgc gccgcgattc gtaccggtcg gcgagctgcc gccgggaacc gtcgtcggcg
     4441 agtacaagat cgacgcgaag atcggcgaag gcggcatggg aatcgtctac tcggcgacgc
     4501 atccgctgat cggcaagcgt gccgcgatca aggtgatcag cgccgagctc ggcaccgacc
     4561 ccgtgctcgt ccagcgcttc gtgcaggagg cgcgctcggt caatcagatc ggccatccga
     4621 acatcgtcga cgtgttcgcg ttcggcaagc tgcccgacaa ccgcaactac ttcgtcatgg
     4681 agtatctgca gggcgagagc ctgcgcgcac gactgacgcg cactttcatg tcgacctcgg
     4741 atgcgatcca gatcctcgac gagatcgcag gtgcgctcga ggccgcgcac gagaagggca
     4801 tcgttcaccg cgatctcaag cccgacaacg tctacctggc gagcgtgcgc gggaacttca
     4861 tcatcgtgaa gctgctcgac ttcggcatcg cgaagctcgt caccgagggc ggcatcgcga
     4921 agaccagcac cggcgagatg atgggcacgc ccggctatct gtcgccggag caggcgcgcg
     4981 gcaagaacgt cgactaccgc acggacgtct acgcgctcgg ctgcatgatg tacgagatga
     5041 tcacgggccg cgtgccgttc cagggcgaga gcccgatgga catcgtgatg atgcacatca
     5101 cgacgccgcc ggagaagccg agcacctaca agccagacat tccgccgctg ctcgagcagg
     5161 tgatcctgga gatgctcgac aaggatccgc agaagcgacc gtccctcggg cacctgcgca
     5221 acgtgttcgc cgagctcgtc gcgagcgggc tcgtgctgct cgagcccggc agcgcgtcga
     5281 cgtttcgctc cgatctcatg cgccggcgcg gcgacaccga gaatcgcacg ccgcgcggca
     5341 ccggctcgaa tgcacgcgcg cagacgccga cgcctgcgcc gccggttgca gtgccgaccg
     5401 ggcaacccaa ccagcacacg ctcggccggc gcaggaacgc ctcgcccagc gatgcgccga
     5461 cgcagatcgc attcacgccg ggaccgaaca cgatcactcc ggccaacgag ccgaagacgg
     5521 ccgcgctacc ggcgcaacct gcaccgcgca aatcgcgaac aggcctgatc atcgcgatcg
     5581 tgctggtcgc ggcggcggtg ccggcggtga cgatcgcgct cggcttgatg aagcagcacg
     5641 acagtgcgcc ggccgatgcc gcggcgcacg tggtggccac ggcgagtgat ggcgcggtcg
     5701 cggcggtggt tgccgatgcg ccggccgctg atgcggctgt tgccgctgtc accgtcgatg
     5761 cagccgtcgc cgcgcgcgac gtcatcatcc gcatcaacgt cccgagcgca aaggtcgaga
     5821 tcgacggcgc gcccgtgctc gatgacaagg gcgtcgtccg cgttcagctg accgatggcc
     5881 cgcatcgcat cgccgcgagc gcgtcggggc ggacgccgat cgacaagacg gtggacgttt
     5941 cggcatcggc gaatgcattc tcgctcgtgc tcgagcgcgc gaaatcgaac acgaagcagc
     6001 acccagccgg gcagggctcc ggctcgggcc aaggctcggc ggtgcagaag gactacaccg
     6061 tcgatccgtt caaggagtag aaccccgcgg gaatctccgc gtgtggttct gagtcttcag
     6121 aacgaccagc cgacggcgaa cgtcgtgccg tgcgcggtcg ggttgatggc gacggtcgtc
     6181 ttgctcttct tcatcgtgcc gagcacgaac agcgtcgcgc cggtcgcgag agcagcacca
     6241 ccggcgatga acgacacgat cgcgatcgtg ttgttccgct tgccagtgtc gtacttgtcc
     6301 tgctgctccg gcgtccacgt gccgtgatcg ctgctgaggt tgttgagatc tttctcggcg
     6361 gagcgcgcca tgctgccgaa gatcacgccg gtgatcagga gcgccgcgcc ggcgccgccc
     6421 gtcacataac cggcggtgag cagtgactgc gctttgactc cgccatcgtc cgtcgtggtc
     6481 ggccccgcgg tggtcgtcgt ggtggtcgtc gtaccgttcg gcgccgtcgt ggtcgtcgtc
     6541 gtcgtgccgc cctcgggcgt catcgtaccg atcggcttct tctcgccgat cttcttctgc
     6601 tcctcgatcg cgtcctgcat ctccttcatg cggtcctcga cgtcttgccg gttcggcgcg
     6661 tcgggcttga gacgcaggta cgtctgatag aagtacaacg cctgctcgta gtccttcttg
     6721 aggcggtacg actgggcgac gttgaacaac aggcccggct tcgacgagat cgcgtacgcg
     6781 gccttgaact cggtgatcgc cgggtcgaac tcgccgaggt tgtagtgcga gatgcccttg
     6841 tcgtagtggg ctttcgcttc cgcttccttg tcagcagtcg ccggcgtccc ttgcggtggc
     6901 ggcgtgggcg gcgcgggttg tgcgagcgcg ctcgtcgccg aggcgacgat catggcgagt
     6961 acgaggttct tcgtcattca gcgcttcctc tagaacggat tgatcgtttc gttcttgtcg
     7021 gtggtcttgg tcgtcgtcgt ggtcgtcgtg gtcgcagcgg aaccagtgcc agtgcccgtc
     7081 ggcgtggtct tggtctgcgt cgtgtttgtc ttcgtgccga ccgtcgcctt cttcgtcgag
     7141 ctcgacttcg agcgctcgag cttggcgacg atctgcaccg atccaccggc cttcgtgtcg
     7201 accgtctgct cgaacggctc gcgaccggcc gcggtcacct gtacgaggtg actgccttcg
     7261 gtctccacgg tcgccttcgc agtcttgcct ttgacctcga cgggattgcc gtcgacgctc
     7321 acctgcgcct tgtcgacgct gatcacgatg ttgatcgtcc cgggctcggg cttcggcgct
     7381 tcgaccttta ccggctccgg cgccttcgcg ggctcgggcg tcttcgctgg ctctggtgtc
     7441 gtggccggct ccggcggctt ggcaggctcc gtcgtcgcga ctggcgcttc cttcggcttc
     7501 tcgccaccgc cgccaccctt caacgcgaag aacgcgccgg caccaccgag caacaccacg
     7561 gcggccgcga tgccgacgat caagccggtc ttcttcttcg gcgcctccgc cacctgcatc
     7621 ggcggctgcg tgcctggcgg atgcgtgctc ggcgcaaccg tcgccggctt cgtgccgacc
     7681 gcgagattca tcgtgcccgg acgcggcgtg ccaccggccg gcgtattcac gcggctgctg
     7741 atgttcgagc cgaatgcgat gccacgtccg ttgttgagtg tgacgagacc actcgtcacg
     7801 agctcggcga acacggcgcg cacttcgggc agcgacggac gcgccgacgg ctccttctcg
     7861 agcatgcgtg cgatcagatc gtcgagcggc gcgggaatct cgggccacac cgagctcgca
     7921 cgcaccggcg gcgtggtcat gtgcatcagg atgatgtcca tcgcgttgtc agagatgaac
     7981 ggcagccgac cacacacgat ctcgaacacc atgcagccga gcgcgtagat atccgtgcgg
     8041 tagtcgacgt tcttgccgcg cgcttgctcc ggcgacagat agccgggcgt acccatcatc
     8101 atgcccgtgc gcgtcttcgc gatgccgccg ccgctgcccg ggccgtcggg cgtcgagagc
     8161 ttggcgatac cgaagtcgag cagcttcacc atcgtgcggc cgccgcgaac gctcgcgagg
     8221 tacacgttgt ccggcttcag atcgcggtga acgatctgct tttcgtgcgc ggcttcgagc
     8281 gcgtcggcaa cctgatcgag gatctcgatc gcttcgccga gcggcatctc tgcgcgctgc
     8341 agacgatccg cgaggctctc gccttgcaga tactccatca cgaagtagtt gcggccgtcg
     8401 ggcagctcgc cgaacgcgaa tacgtcgacg atgttcgggt gaccgatctg gttgaccgag
     8461 cgcgcttcct gcacgaaacg gccaaccgcc gacgcatcgg tgccgagcgc tgcgctgatc
     8521 actttgatcg cggcacgctt gccgatcatc gggtgcgtcg ccgagtagac ggcacccatg
     8581 ccgccttcac cgatcttctt gtcgaccttg tactcgccca cgacggtgcc gggaatcagg
     8641 tcggtcgttg gcggagcctc ttcgacaggc gcatgcgcga cggccatcgc ggcgctggcc
     8701 ggagccgcga cggctgccat cccaccggtc ggcggcgcat acgtcgcgtc catctccacc
     8761 gtgagcgacc acccgtcgtc cgggcacgcc gtcacgttat ctggatactg tttgttgcac
     8821 ttacgacaac gcctcatgtt ccctctcagg aagccccagc ttcattcttg cagacacaag
     8881 atgctgatgc tcgtgaatcc gcagtccttg tagctcgggt tgttcggacc taggaagctc
     8941 ggtgacgcgc cgctgacacg cgacatgttc gcgttgcccg tgcgagcgtg catcgacgcc
     9001 gccgatgtct ccgtgtagtt ggtgcagtta tcaccgccgc ccgccgcgga caccgtagtg
     9061 aagttcggcg cacccgccca gttcgcccag ttggactggt acgagccatc gataaacatg
     9121 gcgggcggcg tatcgaagta cgtggccgtt cccaacgcgg ttggcgtcgg cacgagcgct
     9181 atgttgtcca ggcgatacca ggtgccgagt gacgtgctcg tgttgaagcg cgagatcgca
     9241 gtagcactcg ttgtcgcgag cagcgccttg tacgtgcccg gcagcgcacc gccgacgtgg
     9301 ttgttcgcgt ccatctgaca cttcgcgtca gcatcggcga tgccgccgcc actcgcccac
     9361 agccgcatga acgcgcggcg gtacgtaccg gtcggcgccg cgacggtcac gacggcctgc
     9421 cggttggtct gcacgcaaag caggtgcatc gtcatcgccg agcagccgac cgtaccgaag
     9481 ttgtatccgc tcacgctcgt cgcttgcgcc tcgcccttcg tgacgttgcc tgtcgtggtc
     9541 gagtagttgg tgcaatcgcc ctggccggag tagacaccgt tcgtcgtcgt accggtgacg
     9601 tactcgacgt ccccgaggtc tgcgccggcc tggttgaaac gcgccggcgt gaaccagcgg
     9661 ccattgagca agtcggcctt cgtgttcgca aacggtcgac cgtcggtgcg cacccagccc
     9721 gatgcgctgc caatgcgagt ggcggcgtca gacgatgacg acgacaacaa cgcgacccac
     9781 gtcgaaccag cgccgccgag tggcggcgtg aacccgttcg cgatgttctg acagatcgtg
     9841 tcggcacctg acgtgccgcc gatcgtattc agcgcatacg tcgcgttcgt gacgaatacc
     9901 cggttcgcag ggaccgcgtt gaaggtcgcg gtcacggtct gcgcggcggt catcgtgacg
     9961 gtgcaggtcg tgggtggctg ggcgggcggc gccggcgtgc agttggccgg cacgctgccg
    10021 gaccagccgc cccacgtcga gccgtaggcc gccgtcgcgg tcagcgtgac catcgtgttg
    10081 tagttgaagc tctgcgacgc actcgtgcat gtcgggccac agttgatgat gcccgggctc
    10141 gaggtcacgg tgccggtgcc ggtgctgctt cccttgttca ggttcagcga gaacgtctgg
    10201 agctggaagt tcgcgatgat gttgtaggcc tggttcatcg tgaccacgca gctcgggtta
    10261 ctcgagcaac ccggggcgcc agaccaactc gtgaagttgg tgccggtggt cgccgttgcg
    10321 ttcaacgtga cctgggtgcc gtcgttgtag gtcgacgagc aggtggcgcc gcagctaatg
    10381 ccgcccggcg tcgaggtgac cgtgccgttc gcagctccgt tgccttgctt ggtaacggcg
    10441 aggttccacg tcgtgacgac aaagttcgct gtgacgttct tcgcctgatc catcgtgacg
    10501 tcgcagctac cgttaccggt gcagtctccc gaccagccgg tgaacgtgaa gcccgagcgt
    10561 tccgacgcct tgagcgagac gaacgtcttg tagtcgtaca gcgcgctgca cgacttggtg
    10621 ccgcaatcga tgccgccatc cgtcgtcgag aagacatcgc cagcgttgtt attgttcttc
    10681 gtgaccgaca acaagtattg ctgcggctcg aagtcggcgt acacgccctt gtcggcgttc
    10741 atcgtcaccg agcacgtcgt catcgtgccg catgcggcgc cgtcacccgc ccaaccggtg
    10801 aactcgtatc cggggtccgg cgtcgctgtg agcatcacca cctgaccgtc gttgtagagc
    10861 tcgtcgcagt cggcgccgca gttgatgccc gccgggctcg acgagatggt accgagaccg
    10921 ttgccgatcg gacgcaccga caggctgcgc tgcacgagcg agaagttcgc ggtcaccgtg
    10981 gtgttcgcgg tgagcgtcgt gacgcacgtg cccgtacccg agcagccgcc gccggaccag
    11041 ccggtgaaga ccgcgttggc agcgggcgtg gcgctgagcg tgaccgccgt gccgtagtca
    11101 aagttggcgt tgcatgttcc accacagttg atttgctgct gcggtggcgg cggtgacgtc
    11161 tgcgtcacgg tgctgccggt cgcgccgtta cccgccttca tcaccgtgag catatggacg
    11221 ttcagctgga acgtcgcgga tatgttgacg gccgcaccga tcgtgaccgt gcacgtgctg
    11281 gtgccgctac agccgccacc cgaccagccg gtaaacgtgg agcccgtcgc cgccgtcgcg
    11341 gtgagcgtga ccatcgtcat gttggtgaag ttgaagctgc aggtcgcgcc gcagctgatg
    11401 cccgccgggt tcgacgtcac ggtgccggag ccattgccga gcttcgtgac cgatagcgac
    11461 tgcgagccga gatcaaacgt ggcggtcacg gtcttcgcag catccatcgt caccgtgcac
    11521 gagccgtttc cgggacagct ggccgccgac cagccgacga agaacgaggt cccagaaggc
    11581 accgcgctca gcgtgaccat cgtgccatag gggtacggtt cggtgcagtc gctgccgccg
    11641 ttaccgcagt tgatgccggg cgggctcgcg gtgacgttac cggtgccgct accgccgttc
    11701 gtcgcaacgg tcaacgtcgg tgtcgatagc gtgaacgtgg cggtcacggt ggtcgctgct
    11761 gttaccggca ccgtgcacgt catcgccgaa ccggtgcatc cgccacccga ccagctcgtg
    11821 agcgacgagc cggcttgggg ggctgcgctg agcgtgacca tcgttccgta gtcgtagagc
    11881 tcggtgcagt cggcgccgca ggtgatgccg cccgaaggca tcgtcgacgt gaccgtgcca
    11941 gcgccggtgc cggccttcat cacgctcaac gagtactggc tgagcgtgaa ctgcgcagtc
    12001 accgtgagcg ctgcggtgat cgttgtctgg cacgagccgg tgccgctgca gccgccgccc
    12061 gtccagccgt cgaacgtcga gccggcagac gggttcgcgt tgagcgtgac gatcgtgccg
    12121 tagtcgacgt tctcggtgca gtcggcgccg cacatgaagc ctacgcccga gatcgttccg
    12181 tttccggtgc cgctcttcat caccgtgacc aggtattggt tgagcgcgaa cgtcgcggtg
    12241 acgtcggtcg ctgcgccgat cgtgaccatg cacgtcgtgc ttgcgcccgt acatgcaccc
    12301 gaccagcccg tgaacgtcga gccggtcgcc ggcgcggcgg tcaacatgac catggtgccg
    12361 aacgtgagtg actcggtgca gtcagtgccg cagctgatcc ccgcgggggt cgacgtcacc
    12421 gtgccgctgc cattgccggc tttcgacacc gtgaggttgt agttgctcag cgtgaatatc
    12481 gcggtgacgg tgttcgccat cgtcatcgtc acggtgcacg aggacgtgcc cgcgcacccg
    12541 ccgcccgacc agccggcgaa cgtcgagccc gtagcgggcg cggcactcag cgtgaccatc
    12601 gtgccgtagt cgaacatctg cgtgcacgtg gtgccgcagt tgatgccggc cggcgtcgac
    12661 gtcactgtgc cgccgccggt gccggacttc tgcacggtca agctgtgctg gatgagtgtg
    12721 aacgtcgcgg tgaccgtcgt cgccgcagtt accgtcacgg tgcaggtgcc cgtgccggtg
    12781 cagccgccgc cgctccagcc cgtgaatgtc gagccggtcg acggtgccgc ggtgagcgtg
    12841 accatcgtgt tgtagttgta cgcatcggtg cagtcgacgc cgcacgagat gccggtcggg
    12901 ttcgacgtga cggtgccctg accgttgcct gcgttcacga cggtgagcga aagctgcgtg
    12961 agtgagaacg tcgcggtcac catcgtcgcc atgtcggtcg tgacggtgca cgtgccggtg
    13021 cccgagcagc ccgcgccggt ccagccgtcg aacgtcgagc cggtgccggc aaacgcggtc
    13081 agcgtgacca tcgtgttgtt cgggaactga taactgcagt tggtgccgca gttgatgcca
    13141 gcggggttcg acgtgacaag gccgctgccg ttgccgccga gcgtgacgac gagcgagtta
    13201 tcgagcgcga agtttgcggt gacggtgacc gcggtcgaga tcgtgatcgt gcaggcgccg
    13261 gtgccgctgc aggcgccgct ccagcccacg aatagcgagc cggtcgtcgg cgtcgcggtc
    13321 agggtcacca tcgtgccgga cgtgaagctc tccgtgcagt cggtgccgca gttgatgccg
    13381 gcggggttcg acgtcaccgt gcccatcgag ttgccgccaa gcgagatcgt cagcgggaac
    13441 gtgttcgatg catcgatgcc ggggttatca gcgcggcaga caccgccgta acaatcgagg
    13501 cccggtgggc actcgccgtt cggtccgcac atgatttgtc cgtcttgaaa ttgcggcgag
    13561 aagcacgcag cgacgaacac caacgataga agccaccagc aacgcagcac caaatggttc
    13621 atgtttcgtt tctgctcgcg atccgcttcc ctaagcgtcg cgatgccccc gcaagtcgaa
    13681 gcactctaac actgcgcaga tgtagggaac taggtgtagg ggcgtggagg tagacttggc
    13741 gcacatgcgg cggtggatcg cagcggccgt tctgatgatc gttccgttcc tcgtctgcgg
    13801 gcgtcagtca gggccagcgc ttcgcgtcgc cacgttcaac atcgaggatt tcccaaagaa
    13861 ccgacgccag attgacggcg cgttcgccga gatggccgcg ctgggcgctg gaattatcgc
    13921 ggtacaggag atcggtgagc ctggactgtt tgctcacgag gcgcaccggc tgttgggtga
    13981 acggtggggt ttcgctcacg tcgacacccg gcccatcggc gagacgcggc gcgggcacca
    14041 catcggtgtg ctgttcgacg gcgcgcgctg gcagcggatc ggcatgcgcg ttcacgatgc
    14101 gacgcgcttg gaaggcggtc gccacaaacc aacgctcgag gtccggctgc gcagtggtga
    14161 tctggtcgtg cacgtgttgg tcgtgcatct gaagtcgggc agcgatgggc gcgacattcg
    14221 cgcgcggcag ttcgctgcac ttgccgggat cgtgcgcgag gtacagcgcg caggtgaacc
    14281 gatcgtgcta gccggcgact tcaacgcaac cgagcttggc gatcgcgacg atctcgatcg
    14341 actcgcacgc gacaccgggc tcgtgtgggc gagcgagccg cttgcgtgca gcgcgttttg
    14401 ggcgcgcgat gatgggtgtc cgcgctcacg tctcgatcac atgctgacgt ggaagaagcc
    14461 gcaaagcatt cgcgcagcag gagcgtgcgc gacggaaggc tgcgattggc aagcaagctg
    14521 ccccgtatac acgcagcacg tctcagatca ttgtccggtt gtcgttacat tcgaatgaga
    14581 ttcgaaactt gcacgtgcaa ccgatccggg ggacggtgcg cgcatgaggt ttgccgtcgg
    14641 gctcgtgtgc gtcgctgcgt gcgcgacgcg gcaagcgcca ccgccgacga acgtcgagca
    14701 cgcccaaccg gcgaaggtcc ggcctgcgaa ggtgcggacg gcgaaggcgg cgaattcgaa
    14761 tgcgccgcgc acgcatgccg agctcacgcc ggacgtcgtg ctcgcgacga ttcgcagccg
    14821 ctacatcggc ggtgtcgagc gctgctatcg ccgtcatctc aaaaatgatg cgaccgcgcg
    14881 cggccgcgtg ctcgtgtcgt tcacggtcga tccgaaggga cgcgcgcgcg atggcgaggc
    14941 acacggcatc accgaacgcg tcgacgactg catcgcggcg caagtcgcgc ggtggcagtt
    15001 cccgaagccg gggcaggagt cgcgcttcgc gctcggcctc gagctcggcg tagactagag
    15061 ctctgagcgc gtcgtccaca acgggcgtct ttatctgccg gcacgtctat gacgggctgc
    15121 ccgtgttgct cgtgtcgcgc gtcgacggcg actggcagct gctgtgcgga cctcgatgcg
    15181 gatccgacgc tgcgcgagat tctcgacgtg ggtgcgtcgg ccagcgccgc ccgcctagct
    15241 aaggctcgcg cctcgcgctc gttcgtattc gatcttagcg gcgcgagatc gcccacatct
    15301 cgtcgtgctc ggcgcgatcg agtgcgggca tcgcttgata cagcgcgatc gcggcgcgcg
    15361 cggcggcttg cgcttgcgtg tcgctcgcgt gcgcgagctg cgtgaacacg tgaagcgcgg
    15421 tgcgcgtcgg ttcgttgccg agtgcggcgg cggtcttcgc gaggatgtcg gcagccgcgg
    15481 tgcgatcacc ggcggcgagc tgggcactgg cgagcgcaga cgcgagcgca ggatcctcgc
    15541 cgggtttgta gcgagcgcgt agcgtcgcag cgagtgcgcg cgcttcgtcg gcgcggttgg
    15601 cgttttgcag cagtgcgatg cgtgcggcga gtgcacccgc gcgttcgatc gacagtagct
    15661 cgcccgggcc gagcggcgcg atcgcatcga ggtcgacggt gccgccggcg gcgaagcggc
    15721 gcgcagcaag ctcgaggcgc ggatcgagct tgatggcctc gtcgtacagg atgagcgcgc
    15781 gttcctgatt gccagccgcc gatgcgagct cgccctgggc cgcgacgatt tcgccgacgc
    15841 gcgggctcgt cgcgccgaac gtcttctggc ggatcgccct cgcgtcgtcg agatcacggc
    15901 gtgcaccctc gatgtcgccg gcctcgagct tggtttgcgc gcggtagagc aggccggtcg
    15961 cgaccgcgcg gtcttcggtg ccgaacgccg cgatgcgaag ctcgatgctg cgatcgtgga
    16021 gctgcagcgc ttcgcggagc ttgccgcgcg agcgcttcgc ggccgcgagc ttgtcgatgt
    16081 agatcgcgag ctcggggtgg ttgtcgccga gcgcggacag cacgcgatcg taggcgcgct
    16141 ggaacaacgt gagcgcgcca gccgagttgc cgacggcaag ctcgatcgag ccgcggttca
    16201 tttcgagtag cgagcgctgg ttttcgcgca gtgggtcggt gctcgcgagc gcacgatcga
    16261 ggagctcacg cgcttcaccg agctggcccg cgttgcgcag cgcttgcgcg acgacgccgt
    16321 cgatgtcggc gccttgccgg tttgctcgct tggcggcggc acgcgcgaac ggcgcttgct
    16381 cgatcacctt cgccgggtcg ccggcgaaca gctcgttgcg aagccggcgc gaccacacgt
    16441 cgagctcgag ctcgggcgcc ttcgcgtcgg ccgcagcgac gagcgcctcg tcgagcttgc
    16501 tgcgcgcgac cgcgccgtcg ccgatccggg cttcgatcga tgacgacagc aagagggact
    16561 cggctcgcac gcgcggcgcg gtaatgccgg cgagcttgcc atcggcctcg ccgacgatgc
    16621 cgcgcgcctc gttgtagcgg ccgagcgcga atgctgccga ggcgcgggcg aggtcgtgct
    16681 cgaccgcggt gacttgctcg cgctgcttgc gatcggtcgg cagcgcgagc tcggccggat
    16741 cggagagtcg ctcgcagtcc tcgatgctcg gtagctcgtc gatcgcgccg ggggcggcga
    16801 ctcgtgcgct cggctcgagc gcagatccgg cgagcgcacc ggtgagcgcg ccgaagtgat
    16861 cgagcacgcg atcgaggcag cgcattcgga gatcgagcag cgcgtcggat tgctcgccgt
    16921 gtctggtcgc ttcgcagacg cgacggtagc tgagctgcca ttgctcggcg cgcgcgtcga
    16981 gagcagcgac ggtttgcggc gcccaccgcg ttttgccgag cgcatcgcgg accttcgtcc
    17041 gctgcgtgtc gttccacacc gtctccgagc gcgcaggacc gccggtgcac gactcgccgc
    17101 gcgactgggt gcgatagatc gcggcgccga ggatgccgct catcgcgagt gctgttgcac
    17161 cgagcacgat cgtgcgcgag cgccggcgat cgcggccaag cgcggtgacg agctcgctca
    17221 tcgatgcgaa gcgtccggcg ggcctcgggg cgagtgcgcg cgcgacgatc gcgtgtagcc
    17281 atgcgggcac cgacgagctc ggcggtcgcg cagtcgacgc ctgcgtcgca acctcgcgta
    17341 ggttcgcgat cagatcgcga tgcgttttcg tcgccatcga tgccggaatc gtcgcggctt
    17401 cgccggaggc ggtgggcgcg agcggcccgt gtgggcggcg gccgtagagc gcctcgtaga
    17461 gcgtgacgcc gaagctgaac tggtcggaag cttcggtcgc ggcaccgccg tcgagcactt
    17521 cgggtgccat gtagaacggc gtgccggcgc tctcgccctc ggtgtcggcg cgtggtgctg
    17581 cgaggccgaa gtcgacaacg cgtgtgcggt cgtcgccgac caggatgttg tccggtttga
    17641 tgtcgcgatg gacgagaccg gcggcgtgcg cggcggcgat gcccgcgccg gcttcgcgca
    17701 tgacccggac gatgtcgcgc cagtccctat gcgcgccgat ccacgtacgc agtgacaccc
    17761 cgggcgccag ctccatcacg atgtagacga ggccgtcgag ctcgtcggcg tcgtagatcg
    17821 tgacgacgtt cgggtgcgcg agcttcgcga gtgcgcgcgc ttcgccgagc acgcgtgcgg
    17881 cttcctcgct gctgcgcagt actttcaccg cgaccttgcg ctcgaggcgc ggatcgtacg
    17941 cagcgaatac cgtgcccatc gcgcctgcac cgatgcgatc gacgagcacg aggcggccga
    18001 gcttcggcgc gtgctcgcca ccgaacaact tcgccttgac cttggcgtgc aagagctcgt
    18061 cgaccaggtc gcgcaacccg tgcatggtat acgaagccgt gcgctcggac ggcgagctcc
    18121 tcacagcatg gcgggcgggc gatgccgcag ccggcgaaga gctcgtcgcg cggcactgga
    18181 cgagcttgtc gcgattctgt cgctcgaaga tcggcgacga cggcgccgac gtgatcgctc
    18241 aagtgtttct tgcatgcgtc gagggccgcg atcgcatcgc gggcgacaac gtgaaggcgt
    18301 acttgtttgc ggtcgcgcgc aagcggctcg ccgagcactt ccgccagacc gcgcggcggg
    18361 ccgacctcga tctcgacgtg tcgtcggtcg ccgatctgcg taccggcccg gcgacacacg
    18421 tcgcgcgccg ccagcgcagt gagctactcc gcgaggcgct cacgcggatt ccgctcgacg
    18481 atcagatcgc gatcgagctc gcatacttcg agggcctcgc gatgaaggag atcgcggacg
    18541 tgctcgagat cgccgagaac acggtgcgca gccggctgtc gcgcgcgaga gaaaagctgc
    18601 gcgcggtgct cgcgacgctc gggtccgccg acgaagcagc gctcgccgaa tcgcagctcg
    18661 acggcagttc gtgatcggtt gcacgtttcg cgactgcgcg gcgcctggtt tgcgacatcg
    18721 gtgtcagagg aggcgcggca tcagcagcaa gtgagcagcc gttacgtgcg attcgtgcgc
    18781 gcgttctgca tgctgacctc gtgagggtgc tgctgatcgc gatcgcgttg ctcgtgccgg
    18841 cgcgcgcggt ggccgacgac gacgaagttc gtcacgacga acgcccgacc gcgtggttgt
    18901 tccgggcgac cccgggcttt ggctactcgt gggatcgcga cgtgatgtgc gcggtccagg
    18961 gcaggatgat gttcggcggc tggttcgtcg ggaaggtcgt gacgccgagc ttggttgttg
    19021 gcggtgcgct gaggatggac gtgaacgcgc tccctgacga tggctgcgtc acggacgacg
    19081 atggcctgcg catgacgatc gggatggtgc tgggccccga gatcggctgg tatccgttcg
    19141 atgccggcct gcacgtgttt gcaggcggcg gctttgcgac gttcgatcac gactcgatgt
    19201 acgcggcata tggcgtcggc ggcacgctcg cggttggcta cgactgggcg tttcgcaagg
    19261 agggcgcgac cggcgcgttt cggttcgggc tcgcggcaca ggttaccgct gtgcgcatgt
    19321 cacacggcca cgacatgacg atgccggcag gtgtgatgac gatcggcacc gagtggcgct
    19381 agcgacgcgt agccgcatga tcgaacggct gcgcggcttc gagctgcgcc gcgacgcgga
    19441 acagcaggtc ctcgcgcccg tacgccgcga cgagctggac gccgatcggt aggttggccg
    19501 cgttgcgata cagcggcacc gagcacgcgg gctggccggt cgtgttgaac aagagcgtga
    19561 acggtgcgaa gtcaccagcg cgaaagatgc cggcgaggat gtcgtccggt ggcgacttga
    19621 acgtgccgag cggtggcggt ggttcggcga ccgtcggtgt cagccataga tcgtagcgct
    19681 cgaagaacgt cgcgatgcgg cgcgacgccg cgcgcaacca atcccacgct ttgacgtacg
    19741 acgccgcgct gacgagtcgg ccgagctcgg cgaggcccca ggtcagcggc tcgatctcgt
    19801 gcatccggat cgtgcggccg agcttctcgg acgcggagtc gagatcgtgc gtcacgccga
    19861 cggcccacgc cgcgaggaag ttcggcaccc agccgggatc gctcgtcgct gcgatctcgg
    19921 cgtgctcgac gtgatggccg agcgactcga gcagcttcgc ggtgtgctgg accgcggcga
    19981 tgcagtcggg atgcgagtcg acgaacgtgc cctcggtcgt gatccggcgc gtggcgaagc
    20041 cgatcttgag cttgccgggc gggaccgaga cctcggcggc gtacgggccg agctgtggtg
    20101 gcgcggtgta cggatcgccc ggttgtacgc cggcgagtag atcgaggatc gccgccgagt
    20161 cgcgcaccga ccgcgcaacg acgtgttcgt tgacgagccc gttgttgacg tcgccgtagt
    20221 tcggcgcgaa tgacacacgc ccgcggctcg gcttgagccc gaacaggccg cagcagctcg
    20281 cggggatgcg gatcgagccg acgccgtcgt tcgcgtgcgc gaccgggacc atgccgctcg
    20341 aaaccgctgc cgccgagccg ccgctcgagc cgcccgtcga ccgcgtcgtg tcgtaggggt
    20401 tgcgtgcagg tggccatgcg ggtggctcgg ccgagggcac gatgccgaac tcgctcgtgt
    20461 tcgtcttgcc gacgatcacg aagccggctt tgcgtagctg actgacgagg tagctgtcga
    20521 ccggtgaggt gtagttcgcc tcgcgaagcg gcttgaggcc ggcggtgtag agcacgccac
    20581 cgacggtcgc gaggatgtcc ttgatgagaa tcggcacgcc gcgaaacggc ccatgcggtg
    20641 cgtcatcggc cgttgcgcgc gcctgctcga acagcgggat gatcaccgcg ccgagtgctg
    20701 gattgtgctt ttcaatacgg gcgatcgcgg catcgaccag ctcacgcggt gatgcatcgc
    20761 cacggcggac cagctccgcc tgcgcggtgg catcgaggtc ggacaggtgc atgaggacag
    20821 gagggtaacc gtgatatgcc cgggccatgc aacacctcgc cggaaagctc gcgctcgtga
    20881 cgggcggagg acgcggcatc ggcgccgctt gtgcgcatgg gctggcggcc gctggcgcca
    20941 aggtgatcgt gtgcggccgc tcgcagccgg acctcgagcg cgtggccaag gagattggcg
    21001 gtaccgcgat cgtggccgac ctcgcggatc gcgccgcgac cgaccgcatg ctcctcgagc
    21061 ttcccggccg catcgacgtg ctcgtcaata acgcgggcgt cgccgagagt gcggcgctcg
    21121 atcgcacgaa cgacgagctg tgggaccgca tcctcgagat cgatgcgacg gcgccgttcc
    21181 gcgtgatccg cgcgctcgtg cccgcgatgg tgaaggccgg gtggggacgc gtcatcaaca
    21241 tcgcatcgaa cgccggcgtg tccggctacg gctactcgtc ggcctactgc gccgcgaaac
    21301 acgcgatggt cgggttcacc cgtgcgctcg cgatcgatct cgctcgcacc ggcgtcacga
    21361 tcaacgcgct gtgcccgggc tgggtgcata cgaaaatgtc cgacgaggcg gtcgcccgca
    21421 tcgcggccaa gacgggccgg agcgccgacg aagcgcgcgc gagcctcgaa gcgatgagcc
    21481 cgcaacgccg catgatcgag ccagaagagg tcgcgcatgc cacggtgatg ctgtgcgccg
    21541 aggcgtcgcg cggcattcac ggccagacga tcgtcatcga cggaggagcg attctaaaat
    21601 gagcaccgaa ggcgtcgtcg tcccgggctg gcccgcaccg aagggttact cgaatggtcg
    21661 cgtggggcgc ggtccggtgc ttcacgtcgc tggccagatc ggctggacgc cagacggtac
    21721 gttcgaggcg aaagatctcg tcggccaatt cgcagtcgcg ctcgccaacg tgatcgaggt
    21781 cgtgcacgcg gcgcacggca aggtcaccga catcgccgag atgacggtat acgtgaccga
    21841 catcgcggcg tatcgcgcgg cacgcaagca gctcggtccg atctggaaag agcgcatgca
    21901 gagtcacttc ccggcgatgg cgctcgtcgc tgtctccgcg ctcgtcgagc ccgaagcgct
    21961 cgtcgagatc caagctgtcg cgtacctgga gcacccagaa tgattccgac accgaagtca
    22021 tttgctctcg agctgtccag aggcgtcctc acgatcacgc tcgatcgacc gaagcgcctc
    22081 aatgcgctca cgttcgaggt ctaccaggag ctcgccgaga cgttcgagaa gctcgacgcc
    22141 tacgaggagg tccgcgcgat cgtgatcacc ggccgcggtc gcgcgttctg ctcgggcggc
    22201 gatcaggacg acatcatcaa gcacctgctc ggccgcgaca cgccggcgct gctcgcgttc
    22261 acgcgcacga ccgggcggct gatcgccgcg atccgccgct gccggcggcc ggtgatcgcc
    22321 gcgatcaacg gcgtcgccgt cggtgcgggt gcggtgatcg cgtgcgcggc ggatctgagg
    22381 atcgcggcgt cggcggcgaa gttcggcttc atcttcccgc cggtcgggct gtcgggcgcc
    22441 gacatgggga tcacgtatct cctgccgcgc atcgtcgggc tcggccacgc gtcggagctg
    22501 ctgttcttcg gcgacatcat cgacgccgag acggcgcacc ggatcggcct cgtcaaccgc
    22561 gtggtccccg acggcgccgc cgcgctcgcg atggcgcgcg agtggg
//
LOCUS       DASUFX010000643        20446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_937432,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000643 DASUFX010000000
VERSION     DASUFX010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20446
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937432"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFV99_26020"
     CDS             complement(<1..1232)
                     /locus_tag="VFV99_26020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5062868.1"
                     /translation="MRRTASVLATTFALATTASAHPKHPAGDPSEPTGGEVQPPDVPG
                     TQPRPAAPQTQPAPVQGLNEAQLEALANRAAEETIVVEDEAPAESASSIHLDHQKLGQ
                     RSRTQMSDVLRQVPGLMVSQHAGGGKSDQYFIRGFDADHGTDIAVFADGVPVNLTSHG
                     HGQGYADTHWLIPETIDAVDMHKGPYAARYGDFYTAGALELKTLDSIKGSALWLAGSS
                     PLASTRGFSSFDRRMVGMASPSLRRNPDDKTLLAIEIGEADGAFVNPQDFQRAIAMTK
                     WQGKVGPGRLKLETNWYTGRWNQSGQIPQSLVDQGKLDRFGAVDPSEGGRASRGSVAL
                     GYDMPIGAHGKLLFASYGVGNKLDLFSNFTLYARDMEHGDQIEQTDARLLYGLDAKYE
                     HTIHGSAFDAFVTSGVQLR"
     gene            complement(1291..2139)
                     /locus_tag="VFV99_26025"
     CDS             complement(1291..2139)
                     /locus_tag="VFV99_26025"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX5062869.1"
                     /translation="MKRGLLGAALVAMVCCGPKSGAKNPHPELTTEAVLSELAKAREE
                     LTSFTGQSTMDYWLSGQRMKGDVLVMGKTGKFVRFAALSPAGGSTIAEMACDGTNFVY
                     VDYQNNCALTGPCDARSVAMFFGMALEPDDFVHLALGTPPTLGGNPTGKLTYDGKQKV
                     ELTSGEGTQKITIDTTPAHWNVLDSSLVGTDGKQRWSVANTDFKKVEGSGERAVPGKT
                     HFKSPVQSQDLLVEWQEMKVNVNPPTANFQIQVPQGLATCGQQPAKSPAPAPAKPAPA
                     PAKPKQ"
     gene            complement(2215..2676)
                     /locus_tag="VFV99_26030"
     CDS             complement(2215..2676)
                     /locus_tag="VFV99_26030"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5062870.1"
                     /translation="MRPALLVILAACQSAASSSPAPSPAPVQKVAPESAPPAQSEALT
                     AQVARGKQLFADRCSDCHGDAGQGTDDGPTLVGATALPKQPRPGAKRTVVFRTAADVF
                     VFASRNMPADDRASLTADEYLAICAFALTANGVTLERPLDQTGAQAIVLHP"
     gene            complement(2680..3303)
                     /locus_tag="VFV99_26035"
     CDS             complement(2680..3303)
                     /locus_tag="VFV99_26035"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5062871.1"
                     /translation="MTAVPKLEELTDQQLVERIRGGAREDFEILVRRHNQRLYRAARA
                     IMKSDDEAEDIVQQAWLEVFRNLTQFRGDAAFTTWATRIAVNAAITHARKRPIIAEVP
                     ETIADAKPDEDVERAELGKVLEECLELLPQGNREVMVLRDVLELDTAETATLLGLSEE
                     AVRVRLHRARAAVAAELTERMISKVYSFAGERCARITRNVMAAITLQ"
     gene            complement(3334..3672)
                     /locus_tag="VFV99_26040"
     CDS             complement(3334..3672)
                     /locus_tag="VFV99_26040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062872.1"
                     /translation="MRDLLQAAKTNNRDLVFELLTPATRARLDAEAKRATDLVGAATR
                     YTAKDLISIGASDNVAAPTDITVIEQRGDHAIVEIVSAAGRARLQLFKIGGRWRVDLP
                     QYGAPSQPSL"
     gene            3959..5098
                     /locus_tag="VFV99_26045"
     CDS             3959..5098
                     /locus_tag="VFV99_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062873.1"
                     /translation="MSLRRVAHVLGAVGLGLALQACVVRETRPVNAGYGYGYGYQARA
                     TVTVGAPNPYYVSSMPPQPLYESMTPSPGYGYAWLDGYWHWNGYEWVWISGRWERQQE
                     GYVYIQPYYDYSGGSYVYTPGYWTQRDRVPSNWQVRDHRDGRPPSYTPPPGWHSNPPP
                     TVGGNGGGVRPPPGGGGGTVVTPPPVGGGVISNPPPPGGGVRPPPPGNGNWIPPGTPP
                     GQVSPPPGGRPTPPPPGTGVTPTPPRPDRPPPSTGVTPPPRPDRPPPGSVTQPPRPER
                     PPVQPPVGHPPRPDGQLHPPPSDNQGPPGPPPRPIRPTPQPPSAAPAPRPVQPSGPPS
                     AAPSRPSAPAAPATPARPSRPSAPPPNSSTPPPAAGPGSPPPRRH"
     gene            complement(5153..6232)
                     /locus_tag="VFV99_26050"
     CDS             complement(5153..6232)
                     /locus_tag="VFV99_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5062874.1"
                     /translation="MDRNAFHKLLAFGIERGVSDIHFEVGYPPHYRLHGELLGAIKVP
                     PLTASDTESIARMILEDRSITVDFTRRFAEIDVSYSLAQRGRFRASIFRQRGAVGIVM
                     RLIPIQVLSIEQLNLPPVLAEIADARRGLVLITGATGNGKTTTMAAMLRYINETRHAH
                     IVTIEDPIEFIHEPQKCMIIQREVGADTESFRDAMTAAMRQDPDVILVGEIRDRETAS
                     TALKAAETGHLVLSAIHTPDAVATIQRYVGLFETDAQDVTRDRLGDCLQAVVSLRLLA
                     SKDGRRRLPAVEILRVTRTIRECVRSGRLGDIPDLIRKGRDLYSMQLFDQHLIDLVNG
                     GVVSMETAIYASSNPEEFERALRVD"
     gene            complement(6270..8972)
                     /locus_tag="VFV99_26055"
     CDS             complement(6270..8972)
                     /locus_tag="VFV99_26055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5062875.1"
                     /translation="MICVDDDQNNLNALGRVLRARCTVVLATSGEQALELIEQHADVA
                     AVLADLHMPGLPGAELLARVAQIRPHCRRAVVTGFPESEELINAINAGHLHYVITKPW
                     KLTDLLQVVDQLIHTFKLERDNNRLLDELRSVNTQLREHERILEAQLSERGREISAAT
                     EQLAQMGNQLDALTLRDPLTSLYTHRAFQERLREEVARANRYSQPMSLLIADIDQFAQ
                     INYDLGYQVGDEILRKIALIIQEDESPDRVRTSDIVARYSGEEFVLLLPETAKPGALT
                     KAARLRDAVALATMPGNRKLSLSIGVACLPEDAADPEGLLTAAEAALRGAKRGGPGRI
                     HFFSSGDAGPAAAARSRPFGRVREAEVDRFRPYQERMHEVTSILTRDRSLSCLMVDLS
                     RLHRVELDLGVQHHSEIYDHAAAVLDRLRGAVLDPADLICRSGDGDGYLIILAPRGAH
                     RIDLEQLAQSVERAVEEALAPMVSEVLREAPRITVGHARVLGNSLIRPERLTARLVND
                     ASENARNLGERKAHRDRTNLQDVILGEGLASVYQPIVDLGTGDIFAFEALTRGPRGTG
                     FESPATLFAIADEVDLTVELDRACFKGALRNAMTLEPVHRLFVNLLPMSFYDSAFIEV
                     EVGNLLSAAGLTPANIVFEITERLAIENFASFKRALATYTAMGFGVAIDDVGTRHSNL
                     ETVMSLRPHFIKISDVLVRGIARSTVKREMLRSLRHIAETIDAVMIAEGIEHVDDVVA
                     LRDLGLRYGQGYYMARPGPPFPTLPDYVRAELRSVATNSPMPAKISSRAHVDEDDEDD
                     DRDLPVPTAAGSKSAIPRNVFGRPEDEITSDFAIPGMERPRPRASEPPNENTPQHSAA
                     ATTPVPMWQPLSDDEATGEALLSSLKRQENSSEGEPGPSGPGLN"
     gene            complement(9112..10203)
                     /locus_tag="VFV99_26060"
     CDS             complement(9112..10203)
                     /locus_tag="VFV99_26060"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5062876.1"
                     /translation="MRIGVLVLALVAFAPRAFAHQTSVKYIDLAIHDTTVSVELRCAA
                     TDVTEPMGLPADAKPTVEEALAVAGVVPYVQHWLVLTGCTASAPRANRVDDKFIAVTW
                     TATCPRVDAVEVDLGALFALDRRQEAIIQLAKPGSETIQTIVRAEQGKVTLRAGQSPS
                     LLAWIRTGMDHIYDGVDHICFVLALLLVVMLTRSDGREWRIRPFITTLRSTAVVITAF
                     TIAHSISLIAAALGYVELPSRFVESMIALSIAYTAAEDIVRPDVRWRYGLTFGFGLIH
                     GLGFAATLAELLPPRDVVVPLLCFNVGVEIGQLSIVLVALPVFYGVARVAGGERYRHT
                     WLPLLAGIIFLLGLTMLVERILSVKILPM"
     gene            complement(10219..11112)
                     /locus_tag="VFV99_26065"
     CDS             complement(10219..11112)
                     /locus_tag="VFV99_26065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062877.1"
                     /translation="MRWLACLLVACSAPQPPAASPAQPHAVPSNAKPEVREGDRKHVV
                     DSHVHLAFYPVADKLAQHGVDAVVDLASPEWALGKKYPLLVIQSGPMLTRPRGYPLDS
                     WGADGYGIGCGKSACVKQAIDRLVKQGARVIKIALDDKGLDPKLAQVAVSYAHSLNLK
                     VAVHALTDASARVAADIHADVLAHTPVEPLSGDTVAAWKREAQTGLPAVLSTLAAFGG
                     SDDAIGNLQRLRAAGVTVLYGTDLGNVRVDGPSQEEIALLKRAGLDDQAIETAMTTAP
                     WDFWGFSGLVAVPAGKTTDSR"
     gene            complement(11144..11710)
                     /locus_tag="VFV99_26070"
     CDS             complement(11144..11710)
                     /locus_tag="VFV99_26070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5062878.1"
                     /translation="MTGRAAFAVLATAAACGANLGPGGGDDNMQSPDAKVIRDAAIDA
                     PPDARPCMGGTMAQVAPDGSCLVFVATPTTYVNAKAACTAMTAHLAYLKSAALDTFAE
                     QFIGTTNTWIGATDKAVEMTFVWDDGTPLSFTNWGMDEPNNGGTGATYQEDCVIIAGM
                     RAEKQWDDRPCDASEVPTSGNFAYLCQY"
     gene            11749..13182
                     /gene="glmU"
                     /locus_tag="VFV99_26075"
     CDS             11749..13182
                     /gene="glmU"
                     /locus_tag="VFV99_26075"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5062879.1"
                     /translation="MSRQAPLVFIMAAGLGTRMKSDTAKVLHKIAGRPMLHWVVGAAR
                     AAGAERVVAILGHQHETVKAALDASFGAGSVEIALQPEQRGTGHAVQCALPAVTNEPD
                     DRIVVILTGDAPLLVSERIAELVRACAESKSGLALLSTRPDRDMPYGRLVRDQSGVLE
                     RIVEHADATPDQRQIRDTNAGFYAIQLGHLRKDLASLRADNAKGELYLTDLIAAAYKR
                     GGATSIDAPFTEVSGINDRVDLSTVEAEARRRINERWMRQGVTMVDPRATYIDADVGP
                     LGKDVWLGPGVVLRGKTKIGDNVRIDAGAVITDVTVGDDTYIKAYSIVAETVMGERVE
                     VGPFSHCRPGTRIDEDARIGNFVETKKTHVMAGAKANHLAYLGDASIGQKSNIGAGTI
                     TCNYDGVSKHKTTIEAGAFIGSDSQLVAPVTVGRDAYVGSGSTITKDVPRGALALSRV
                     KQTNIEGWADRFREAQAKRKKSSDDGH"
     gene            13201..14529
                     /locus_tag="VFV99_26080"
     CDS             13201..14529
                     /locus_tag="VFV99_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062880.1"
                     /translation="MYCSIDKIDLAARIEGRPVAVQTDHRAYAEIEAEPELSVLYAMA
                     RVLNARTHLFESGHTDAVVHYAVNHEPPALLREAIGAVGGTLERGKRLEVLGAANEML
                     ASELADRSFAALANRVKSRVGSRDVAIALRMLEDQTFTDPPSRDDEPAYWQRVLELAA
                     LAGELLRARYPGHWVQTDRALVPFGFQVQSGAAVMFPTNRAQRVIEDGADESLFKLLL
                     AAEETVQRPTDAMTGRLMPSLRSRSDIELDEVVWRPIVDDQGPLDLPVVVCGIDGEST
                     FGMIRREAMTKTVDAAFEEALMNLGDEEVQCEEIEIAGLTVIMVSGNFYAAEKILDRE
                     FMKSLHEELDSELLAASTPARGCLLVTANYHDNASLARFAAIAHARHEDGGGRAISPA
                     VLLVEKGRVAGYVRGPNVPKERPETVTARAETVPSTPKRVGLWRRLLGRK"
     gene            14526..15782
                     /locus_tag="VFV99_26085"
     CDS             14526..15782
                     /locus_tag="VFV99_26085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062881.1"
                     /translation="MNAELNRQDAGNAKPKKKIFWAWRVSRLGGLSLLLLACSGKAKV
                     PKRDDAAVHASTSDAGTADADKAVFPELAQLPNVEPVRVIALPARPDVPRFNVGGPVL
                     AGDVAVVSSSQFGFIAVDFRGGHIAWTKPAGAHVAPPQLIDGNVVLIGDCINPPEVKD
                     DETLLACMRVVTPTGSDEAYVAVRGKQKVVAAFAGSAGEQHVDATDKGVLWRRGEQAV
                     VIDLVTGVATPAPATEPPLVVTYKEKTWRIRRTEDGVITAEGKPPWHTDRAYGTLLGG
                     VYIPEQSPFVRVASPTRHRGQPEILLFDMDATGSLNSQVSMSPAPGIGLIGHAIDRVG
                     DVALAVRLDTSLERDYIAGYAANALLMWTYPLPRMPRADPVGIAIAHDAVVVFHDGDT
                     FTVLPELSAPPTAPGAVREPSENATP"
     gene            15857..16789
                     /locus_tag="VFV99_26090"
     CDS             15857..16789
                     /locus_tag="VFV99_26090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062882.1"
                     /translation="MSKLLSTVLTATCALSLAATGCIKQDAPPDEIARAIPTAEQVRI
                     KLPNSGKTLGDLANYYVVTRDVTRTLNGGTAWVLVLIHSIVQYPVTSTNGNVYTWGPW
                     SDALAPAEYKLDVTANADGTFDYQLSGRNKTVSGSQFEVVIDGHADPRPGDLQGNGDF
                     QLDFDAGRRVNPVDSGDAKGKVTVQYDLAARHLDMLAQAPDANGNPAMFEYAYNETLD
                     GGGDMVFNATADAGGTALPENIVIRSRWQGDGAGRGDARLAGGDLGTAQAIASECWST
                     LFRRVYYTDNATWQPTEGDVAQCAYGTADLPPLD"
     gene            16890..17243
                     /locus_tag="VFV99_26095"
     CDS             16890..17243
                     /locus_tag="VFV99_26095"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5062883.1"
                     /translation="MATMPKDAQDLFDNLVPEGLKLYPDKARELNAIYCFKITGDGGG
                     EWTVDCTADPPSCTRGDSGKAQCTVEVASDDFKTMLSDPNAGMQLYFQGKLRVSGDPM
                     LAMKLQQLFEIARPK"
     gene            17276..18451
                     /locus_tag="VFV99_26100"
     CDS             17276..18451
                     /locus_tag="VFV99_26100"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5062884.1"
                     /translation="MTGHRASALADALRGIRVLDLSRLLPGPYLTMVLADMGADVVKV
                     EDPKLGDYLRAFPPGKGGMSGRFLAINRGKRSLALDLKAPAARDAFLKMVEKADVVVE
                     SFRPGVMDKLGIGYAALAARNPKIIVCSISGFGQTGPYVERAGHDLNYIALAGVLAMT
                     GPQDSAPQMPGVQIADLAGGALWGATAILGALVGRDRTGKGAHLDISMTEGALALLAA
                     EFGNLFCGATPSRGTEMLNGAAACYGVYRTKDDRYVSVGALEPKFWIALNQAIGRAPN
                     VAEIVDMKLQAKTREDLAAIFATKTLAEWTAFFAERDCLVEPVLEPHEVVDHPLHRAR
                     DVFFEIDGGEGVGPIKQVRTPIGTPAHATPPPRLGQQSQAVLREYGFSDEQIAALTT"
     gene            18557..19858
                     /locus_tag="VFV99_26105"
     CDS             18557..19858
                     /locus_tag="VFV99_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062885.1"
                     /translation="MKHILLAGLLALFLVACGDKKSDGPSCTATGTACANGAADCCTG
                     YCEELTGNCARAPGTCGADGTDCSVGPDCCSLHCDDNFKCSGDACVSDNQDCGGDNDC
                     CSGICTSGKCAPLNPGCKTSGNTCAGHSECCSGFCDNGTCSISPSFCTQNGDVCVNDG
                     ECCGGTCMKPDGATLGTCAVVPSNGATGCDTLGIVCAQGASYDPATPLPTCGGSCCSR
                     ACFPYGPSGVLICQPPSGCRPTGELCYEDADCCGGPGQPDNNPSNVMCRKEAGYAVGR
                     CDNGNSCSPAGAICRLQSVECNANANCCAGNVEQFDTCHQDSLGIPRCGVGVGIDCTN
                     PADHAGMPCASSADCCGLPCVGNPDTGFVCGSTCVTQGGMCTTTADCCAGLPCNVPAG
                     ETVGMCGMPQGCADYGQACTTTADCCNMLPCNNGKCEGIIL"
     gene            complement(20040..>20446)
                     /locus_tag="VFV99_26110"
     CDS             complement(20040..>20446)
                     /locus_tag="VFV99_26110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062886.1"
                     /translation="YVRLLVSFQLPTLPTGAAVERVLLTGSQDSVSGSPYTKLGVVKA
                     QHVTFMTFDSAAFGGAPLVTAGDFSANATVEQKSLDVTGSVADDYTNAGPRAGRSQYR
                     LEFPTGTNNDGVRDMVYFSRLGFSLTLTYHVE"
BASE COUNT         3455 a         6757 c         6773 g         3461 t
ORIGIN      
        1 cgcagctgca cgccgctcgt cacgaacgcg tcgaacgcgc ttccgtggat cgtgtgctcg
       61 tacttcgcgt cgagtccgta gagcaggcgg gcgtcggtct gctcgatctg atcgccgtgc
      121 tccatgtcgc gcgcgtacag cgtgaaattc gagaacaggt cgagcttgtt gccgacgccg
      181 tagctcgcga acaagagctt gccgtgcgcg ccgatcggca tgtcgtagcc gagcgcgacg
      241 ctgccacgcg acgcgcgtcc gccttcgctc ggatcgaccg ccccgaagcg atcgagcttg
      301 ccctggtcga cgagcgactg cggaatctgg cccgactgat tccagcgccc cgtgtaccag
      361 ttcgtctcga gcttgaggcg gcccggaccg accttgccct gccacttcgt catcgcgatc
      421 gcgcgctgga agtcctgcgg attcacgaac gcgccgtctg cctcgccgat ctcgatcgcg
      481 agcagcgtct tgtcgtcggg atttcgccgc aggcttgggc tcgccatgcc gaccatgcgg
      541 cgatcgaacg acgaaaaccc acgcgtgctc gcgagcggtg agctgccggc gagccacagg
      601 gccgagcctt tgatcgaatc gagtgtcttc agctcgagcg cgccggccgt gtagaagtcg
      661 ccgtagcgcg cggcgtacgg gcccttgtgc atgtcgacgg cgtcgatcgt ctcggggatc
      721 aaccagtgcg tatctgcgta gccctggccg tgaccgtgcg aggtcaggtt gaccggcacg
      781 ccgtcggcaa acactgcgat gtcggtgccg tgatcggcgt cgaagccgcg aatgaaatat
      841 tgatccgact taccgccacc cgcgtgctga gagaccatga ggcctggcac ctggcgaagc
      901 acgtcggaca tctgcgtgcg cgagcgttgc ccgagcttct ggtgatcgag atggatcgac
      961 gacgccgact cggcgggcgc ttcgtcttcg accacgatcg tttcttcggc cgcacggttc
     1021 gcgagtgcct cgagctgcgc ctcgttcaag ccctgcactg gcgcgggctg ggtttgcggc
     1081 gcagccggac gcggttgggt gccgggaaca tcgggcggct gcacctcgcc gccggtcggc
     1141 tcggatggat cgccggccgg atgcttggga tgcgcgcttg ctgttgtcgc caacgcgaac
     1201 gtcgtcgcta gcacgctggc agtgcgcctc atcctcatga ccggatgtac gagacgctac
     1261 gcccggtgga tcgagtcgcg caactcggcg ctactgcttt ggcttcgcgg gagctggagc
     1321 gggcttcgcg ggagctggag caggagattt cgcaggctgc tggccacacg tcgccaaccc
     1381 ctgcgggacc tgaatttgaa aattcgccgt cggtgggttc acgttcacct tcatctcctg
     1441 ccactcgaca agcagatcct gactctgcac gggcgacttg aagtgcgtct tgcccggcac
     1501 cgcgcgctcg ccgctgcctt cgaccttctt gaagtcggtg ttcgcgacgc tccaccgctg
     1561 cttgccgtcg gtgccgacca gcgagctgtc gagcacgttc cagtgcgccg gggtcgtatc
     1621 gatcgtgatc ttctgcgtgc cctcgcccga cgtcagctcg accttctgct tgccatcgta
     1681 ggtcagcttg ccggtcgggt tgccaccgag cgtcggcggc gtgccgagcg cgagatgcac
     1741 gaagtcatcc ggctcgagcg ccatgccgaa gaacatcgcg accgaccgcg catcgcacgg
     1801 tccagtgagc gcgcagttgt tctggtagtc gacgtagacg aagttcgtgc cgtcgcacgc
     1861 catctccgcg atcgtgctgc cgcccgccgg cgacagcgcg gcgaagcgga cgaacttgcc
     1921 ggtcttgccc atcacgagca cgtcgccctt catgcgctgc cccgagagcc agtaatccat
     1981 cgtgctctgg ccggtgaacg aggtcagctc ctcgcgcgcc ttcgcgagct cgctgaggac
     2041 cgcttccgtc gtgagctcgg gatgcggatt cttcgctccg ctcttcgggc cgcaacacac
     2101 catcgcgacc aacgcagcac caagcaagcc tcgcttcatg ccgcggtgat agcagagcta
     2161 cgccccccat tcacatcacc gcatgacgtt cctcacgcgc agcggcattc accgctacgg
     2221 gtgtaagacg atggcctgcg ctcccgtttg atcgagcggg cgctcgagcg tcacgccatt
     2281 cgcggtgagc gcgaacgcgc agatcgcgag gtactcgtcg gcggtcagtg acgcgcgatc
     2341 gtctgcgggc atgttgcgcg aggcaaacac gaacacgtcg gccgcggtac gaaacaccac
     2401 cgttcgcttg gcgccggggc gcggctgctt cggcagcgcg gtcgcgccga cgagcgtagg
     2461 gccgtcatcg gtgccctgcc cggcatcgcc gtggcagtcg gagcacctgt ccgcgaacag
     2521 ctgcttgccg cgtgcgacct gcgcggtaag cgcctcgctc tgcgcgggtg gcgccgattc
     2581 gggcgccacc ttctgcaccg gcgctggtga cggcgctggt gacgacgaag cagccgattg
     2641 gcaggccgcc aagatcacga gcagagccgg tcgcatctcc tattggagtg tgatcgcggc
     2701 catgacgtta cgcgtgatgc gcgcgcagcg ctcacccgca aacgagtaga ccttgctgat
     2761 catacgttcc gtcagctcgg cggcgaccgc agcccgcgcg cgatgcagac gcacgcgcac
     2821 cgcctcttcg ctgaggccga gcagcgtggc cgtctccgcg gtgtcgagct cgagcacgtc
     2881 gcgcagcacc atcacctcgc ggttgccctg cggcagcagc tcgagacact cctcgagcac
     2941 cttgccgagc tcggcgcgct cgacgtcttc gtcgggcttc gcgtccgcga tcgtctcggg
     3001 cacctcggcg atgatgggcc gcttgcgcgc gtgcgtgatc gccgcgttga ccgcgatgcg
     3061 cgtcgcccac gtcgtgaacg cggcgtcgcc gcggaactgg gtcaggttcc gaaacacctc
     3121 gagccacgcc tgctgaacga tgtcttctgc ttcgtcgtcg ctcttcatga tcgcgcgcgc
     3181 cgcgcgatac aggcgctggt tgtgacggcg tacgagaatt tcgaagtcct cgcgtgcgcc
     3241 gccgcggatg cgctccacca attgctgatc ggtgagctcc tcgagcttgg gtaccgcggt
     3301 catgatcaat agagagcgta gcccgggatg ccgttacagg ctcggttggc tcggcgcgcc
     3361 gtattgcggc aggtccacgc gccaacggcc gccgatcttg aacagctgca agcgcgcgcg
     3421 tcccgcggcc gacacgatct cgacgatcgc gtgatcgccg cgttgctcga tcaccgtgat
     3481 gtcggtcggt gcggccacat tgtcgctcgc accgatcgag atcagatcct tcgcggtgta
     3541 gcgcgtcgct gcaccgacga gatcggtcgc gcgcttcgct tctgcgtcga gccgcgcgcg
     3601 tgtcgccggt gtaaggagct cgaacacgag atcgcgattg ttcgtcttgg cggcctgtag
     3661 caaatcacgc acggtcattt ccggcccagg ttgtgtgacc tggcagctac gcccagcgac
     3721 cgcagccgcg agtgccatgg cagcgagagc actcgcaact gctgcaatcg ttgaccttct
     3781 tgtgcctaat gcgagtgcca ccgtacgccc cggcattgcg gttgcactct ccgacgctcg
     3841 cggtggttgc ccgtgtagtc ggtttccatc gaggttctgt gtacttgtgg tcaactatct
     3901 cgtcaactag gatgaatcgt tggattggat tgccgtccaa tcacttagga ggctcgacat
     3961 gagtttgcga cgcgttgcac acgttcttgg cgctgttggc ctcggcctgg cgctgcaagc
     4021 ctgtgtcgtt cgcgagacgc ggcccgtgaa tgccggttac gggtacggat acggttatca
     4081 ggctcgcgcc accgtcacgg tcggcgcacc gaatccctat tacgtctcga gcatgcctcc
     4141 gcagccgctc tacgagtcga tgacgccgtc gccgggctac ggctacgcgt ggctcgatgg
     4201 ctactggcac tggaacggat acgagtgggt ctggatcagc ggccgctggg aacgccagca
     4261 agaaggctac gtctacatcc agccgtacta cgactactcg ggcggctcgt acgtgtacac
     4321 gcccggctac tggacgcagc gcgatcgcgt gccgtcgaac tggcaggttc gcgatcatcg
     4381 cgacggccgt ccgccgagct acacgccgcc acccggctgg cactcgaacc cgccgccgac
     4441 ggtgggcggt aatggtggcg gtgttcgccc gccgcctggc ggcggaggtg gcaccgtcgt
     4501 gacgccgccg cccgtcggtg gtggcgtgat cagcaacccg cctcctccag gtggtggtgt
     4561 tcgtccgccg cctccgggca acggcaactg gatcccgccc ggaacgcctc ccggccaggt
     4621 ctcgccgccg cccggtggtc gtccgacgcc gccgccgccg ggcaccggcg tgacgccgac
     4681 gccgccgcgc cccgatcgtc cgccgccgag caccggcgtg acgccgccgc cgcgaccgga
     4741 tcgtccgcca cccggcagcg tcacccagcc gccgcgcccg gagcgtccgc cggtgcagcc
     4801 cccggttggc catccgccgc gtccggacgg tcagctgcat ccgccgccga gcgacaacca
     4861 gggtcctccg ggcccgccgc cgcgcccgat tcgcccgacg ccgcaaccgc cgtcggcggc
     4921 acccgcgccg cggccggtcc agccgtcagg cccaccgagc gcggcaccct cgcgtccgtc
     4981 ggcaccggcg gcgccggcca caccggcgcg cccgtcgcgt ccgagcgcac caccgccgaa
     5041 cagcagcacg ccaccgccag cagccggccc gggctcaccg ccgccgcgtc gccactaagc
     5101 agcgcgcgag agcgtgaacg aacgaagcgg cgccatccgg cggacggcgc catcagtcga
     5161 cgcgcagcgc gcgctcgaac tcttcgggat tgctgctcgc gtaaatcgcg gtctccatcg
     5221 agacgacgcc gccgttcacg agatcgatca gatgttgatc gaagagctgc atcgagtaga
     5281 ggtcgcgacc cttgcggatc agatccggaa tgtcgccgag gcgtcccgag cgcacgcact
     5341 cgcggatcgt gcgcgtgacg cgcaggattt cgaccgcagg cagccggcgg cgaccgtctt
     5401 tgctcgcgag gaggcggagc gacacgacgg cttggaggca gtcaccgagc cgatcgcggg
     5461 tcacgtcctg tgcgtcggtc tcgaacaagc cgacgtagcg ctggatcgtc gcgacggcat
     5521 cgggcgtgtg gatcgcggac agcacgaggt ggccggtctc ggcggccttg agcgcggtgg
     5581 aggcggtctc gcgatcgcgg atctcgccga cgagaatgac gtcgggatcc tggcgcatcg
     5641 ctgccgtcat cgcgtcgcgg aacgactcgg tgtcagcgcc gacctcgcgc tggatgatca
     5701 tgcacttctg cggctcgtgg atgaactcga tcgggtcctc gatcgtgacg atgtgtgcgt
     5761 gccgcgtctc gttgatgtag cgcagcatcg cggccatcgt cgtcgtcttg ccgttaccgg
     5821 tcgcgcccgt gatgagcacg aggccacgcc gggcgtcggc gatctcggcg aggaccggcg
     5881 gcagattgag ctgctcgatc gacagcacct ggatcgggat caagcgcatc acgatgccga
     5941 ccgcgccacg ctggcggaag atcgacgcac ggaagcggcc gcgttgcgcg agcgagtacg
     6001 agacatcgat ctcggcaaac cggcgggtga agtcgaccgt gatgctgcgg tcctcgagga
     6061 tcatccgggc gatcgactcg gtatcggagg cagtcagcgg cgggactttg atggcgccga
     6121 gcagctcacc atggaggcgg tagtgcggcg gatagccgac ctcgaagtga atgtccgaaa
     6181 cgccgcgctc gatgccgaat gccaggagct tgtgaaacgc gttcctgtcc ataagcgaac
     6241 gaccagcggt acaacccgcg aatcggcgct cagtttagac caggaccgct cggtcctggc
     6301 tcgccctcgg acgaattttc ttggcgtttg aggctggata gcagcgcctc gccggtcgcc
     6361 tcgtcatcgc tgagcggctg ccacatcgga accggcgtcg tagccgccgc tgaatgctgc
     6421 ggggtgttct cgttcggtgg ctcggatgcg cgcggacgtg gacgctccat gcccgggatc
     6481 gcaaagtcgc tggtgatctc gtcttcaggg cggccgaaga cgttgcgcgg gatcgcgctc
     6541 ttgctaccgg ccgcggtcgg gaccggcagg tcgcgatcat catcctcgtc gtcttcgtcg
     6601 acatgggccc gcgacgaaat ctttgcgggc atcggcgaat tggttgcaac gctccgcagc
     6661 tcggcccgca cgtaatcggg aagcgtgggg aacggcggcc ccggccttgc catgtaatag
     6721 ccctgaccgt agcgcaggcc gagatcgcgc agggcgacca cgtcatctac gtgctcgatg
     6781 ccttcggcaa tcatcacggc gtcgatcgtc tcggcaatgt gccggagcga gcgcagcatc
     6841 tcgcgcttga cggtcgaccg cgcgataccg cgcacgagga cgtccgagat cttgatgaag
     6901 tgcgggcgca gcgacatcac ggtctcgagg ttcgagtggc gcgtaccgac gtcgtcgatc
     6961 gcgacgccga agcccatcgc ggtgtacgtc gcgagcgcgc gcttgaacga cgcgaagttc
     7021 tcgatcgcga gccgctcggt gatctcgaag acgatgttcg cgggcgtgag gccggcggcc
     7081 gagagcaagt tgccgacctc gacctcgatg aacgccgagt cgtagaacga catcggcaac
     7141 aggttcacga acaggcgatg caccggctcg agcgtcatcg cgttacggag tgcgcccttg
     7201 aagcacgcgc ggtcgagctc gacggtgaga tcgacctcgt cggcaatcgc gaacagcgtc
     7261 gcgggcgact cgaagccggt gccgcgcgga ccacgcgtca gcgcctcgaa cgcgaagata
     7321 tcgccggtgc cgaggtcgac gatcggctgg tacacgctcg cgaggccttc gccgaggatg
     7381 acgtcctgca ggttcgtccg atcgcggtgc gccttgcgct cgccgaggtt gcgcgcgttt
     7441 tcgctcgcgt cgttgacgag acgcgcggtg aggcgttcgg ggcgaatcag cgagttgccg
     7501 agcacgcgcg cgtgaccgac cgtgatgcgc ggcgcctcac gaagcacctc gctcaccatc
     7561 ggcgcgagcg cttcctcgac cgcgcgctcg acggactgcg cgagctgctc gaggtcgatg
     7621 cggtgcgcgc cacgcggcgc gagaatgatc aagtagccgt cgccgtcgcc ggagcggcag
     7681 atcaggtcgg ccggatcgag gacggcaccg cgcaggcgat cgagcaccgc ggcggcgtgg
     7741 tcgtagatct cgctgtggtg ctggacaccg aggtcgagct cgacgcgatg gagacgggat
     7801 aggtcgacca tcaagcacga cagcgaacga tcccgcgtga ggatcgacgt cacctcgtgc
     7861 atgcgctcct ggtacggccg gaagcgatcg acctcggcct cgcgcacgcg accgaacggg
     7921 cgactgcgcg ctgccgccgc cggaccggca tcgcccgacg agaagaagtg aatgcggccg
     7981 ggaccaccgc gcttggcgcc gcgcagtgcg gcttcggcgg cggtgagcag gccttcggga
     8041 tcggcggcgt cctcgggcaa gcacgcgacg ccgatcgaca gcgagagctt gcgattgccc
     8101 ggcatcgtcg cgagcgcgac ggcatcgcgc agacgcgctg ccttcgtcag tgcgcccggc
     8161 tttgcggtct cgggcagcag cagcacgaat tcttcgccgg agtagcgggc gacgatgtcg
     8221 gaggtgcgca cgcggtcggg cgattcgtct tcctggatga tgagcgcgat cttgcgcagg
     8281 atctcgtcgc cgacctggta gccgaggtcg tagttgatct gcgcgaactg atcgatgtcg
     8341 gcgatcaaga gcgacatcgg ctggctgtag cgattcgcgc gcgcgacctc ttcgcgcaac
     8401 cgctcctgga acgcgcgatg cgtgtacagg ctcgtcagcg gatcgcgcag cgtcagcgcg
     8461 tcgagctgat tgcccatctg cgcgagctgc tcggtcgccg cggagatttc acggccgcgt
     8521 tcggagagct gtgcttcgag gatgcgctcg tgctcgcgca gctgcgtgtt caccgagcgg
     8581 agctcgtcga gcaggcggtt gttgtcgcgc tcgagcttga acgtgtgaat gagctggtcg
     8641 acgacctgca gcaggtcggt caacttccac ggcttggtga tcacgtagtg gaggtgaccc
     8701 gcgttgatcg cgttgatcag ctcctccgac tccgggaatc cggtgaccac tgcacgccga
     8761 cagtggggac gaatctgcgc gacgcgcgcc aggagctccg cgccgggaag tccaggcatg
     8821 tggagatctg cgagcaccgc ggcgacatcg gcgtgctgct cgatgagctc gagcgcctgt
     8881 tcgccactcg tcgcaagcac gaccgtacag cgcgcacgaa gcacgcgtcc caaggcattc
     8941 agattgttct ggtcgtcgtc gacgcagatc acaaccggtt tgcctggttc ttccgatacc
     9001 ggctctggag cctcggggac tgccaatgtg ccttccagta taaacagggt ctgatgcgcc
     9061 cggaaccttc cggtgcgcct gtgggttatc gaagtacggc gctacatgcg cctacattgg
     9121 caggatcttc acgctgagga tcctctcgac gagcatcgta agcccgagca ggaaaatgat
     9181 tcctgcgagc agcggcaacc aagtgtggcg gtagcgctcg cctcctgcga cgcgtgcgac
     9241 accgtaaaag acgggtagcg cgacgaggac gatcgacagc tggccgatct cgacgccgac
     9301 gttgaaacag agtagcggca ccacgacatc acgcggtggc aacagctcgg cgagggtcgc
     9361 cgcaaagcca agaccgtgga tcaaaccgaa gccgaacgtg agcccatacc gccagcgcac
     9421 gtcggggcgc acgatgtctt ctgccgccgt gtacgcgatc gacagcgcga tcatgctctc
     9481 gacgaaccga ctcggtagct cgacgtaacc gagcgccgcg gcgatcagcg agatcgagtg
     9541 cgcgatcgtg aacgcggtga tcacgacggc cgtcgaacgc agcgtggtga tgaatgggcg
     9601 gatccgccac tcgcgaccgt cgctccgcgt cagcatcacg acgagcagca gcgcgagcac
     9661 gaagcagatg tgatcgacgc cgtcgtagat gtgatccatg ccggtgcgga tccatgcgag
     9721 caacgacggt gactgcccgg cgcgcaacgt gaccttcccc tgctcggcgc gcacgatcgt
     9781 ctggatcgtt tcgctgcccg gcttcgcgag ctggatgatc gcttcctggc ggcgatcgag
     9841 cgcgaacagc gcgccgagat cgacctcgac cgcgtcgacg cgcgggcacg ttgccgtcca
     9901 cgtcaccgcg atgaacttgt cgtcgacacg attcgcgcgc ggcgcgctcg ccgtgcagcc
     9961 agtgagcacg agccagtgct ggacgtacgg cacgacgcca gccactgcga gcgcctcctc
    10021 gacggtcggc ttcgcatcgg cgggcaggcc catcggctcg gtgacgtcgg tcgccgcgca
    10081 acgcagctcg acactcacgg tcgtgtcgtg aatcgcgagg tcgatgtact tgacgctcgt
    10141 ctggtgcgcg aacgcgcgcg gcgcgaacgc cacgagcgcg agcacgagaa caccgatccg
    10201 catccgcacg cggcggtttt accgcgagtc cgtggtcttg ccggccggga cggccaccag
    10261 tccgctgaat ccccagaagt cccacggcgc cgtcgtcatc gccgtctcga tcgcctggtc
    10321 gtctaagccc gcacgcttaa gaagtgcgat ttcttcctgg ctcggaccat cgacgcgcac
    10381 gtttccgagg tccgtgccat agagcaccgt tacaccggcc gcgcggagtc gctgcaaatt
    10441 gccgatcgca tcgtcactgc cgccgaaggc cgccaacgtc gacagcacgg ccgggagccc
    10501 ggtctgcgcc tcacgcttcc acgcggcgac cgtgtcaccg ctgagcggct cgacgggcgt
    10561 gtgcgcgagc acgtcggcgt ggatatcggc cgccacgcgc gcgcttgcat cggtcagcgc
    10621 atgcacggcg accttcaggt tcagcgagtg cgcatagctg acggcgacct gcgcgagctt
    10681 cggatcgagg cctttgtcat cgagcgcgat cttgatgacc cgcgcgcctt gtttcaccag
    10741 gcgatcgatc gcctgtttca cacacgccga cttgccgcag ccaatgccat agccgtcggc
    10801 gccccacgaa tcgagcgggt agccacgcgg ccgcgtcagc atcgggcccg actggatcac
    10861 gagcagcggg tacttcttcc cgagcgccca ctccggcgac gcgaggtcga caaccgcatc
    10921 gacaccgtgc tgtgcgagct tgtcggcgac cggatagaac gcgaggtgta cgtgcgagtc
    10981 gacgacgtgc tttcgatcgc cctcgcgaac ctcgggcttg gcgttcgacg gcacggcatg
    11041 cggttgcgcc ggtgacgcgg ccggtggctg cggcgcactg cacgcgacga gcagacacgc
    11101 gagccaacgc acgcatgaga tacgcgcgag cccgaacatc ggatcagtat tggcagaggt
    11161 atgcaaagtt gccgctcgtc gggacttcgc tcgcatcgca cggtcgatca tcccactgct
    11221 tctcggcgcg catgcccgcg atgatcacgc aatcctcttg ataggtcgca ccagtgccgc
    11281 cgttgtttgg ctcgtccatg ccccagttcg tgaacgacag cggcgtgccg tcatcccaca
    11341 cgaacgtcat ctcgaccgcc ttgtcggtcg cgccgatcca cgtgttcgtc gtgccgatga
    11401 actgctcggc gaacgtgtcg agggccgcgg acttcaggta ggcgaggtgt gccgtcatcg
    11461 ccgtgcacgc ggctttcgcg ttgacgtacg tcgtcggcgt cgccacgaac acgaggcacg
    11521 aaccatctgg cgcgacttgc gccatcgtgc cgcccataca cgggcgcgcg tcgggtggcg
    11581 cgtcgatcgc tgcgtcgcga atgacctttg cgtcgggcga ctgcatgttg tcgtcgccac
    11641 cgcccggtcc gagattcgcc ccacaggccg ccgccgtcgc aagaacagca aatgcggcgc
    11701 ggcccgtcat ctactttatt gtgcccgatc gtgtaggttt cgggacacat gagtcgtcaa
    11761 gcacccctcg tgttcatcat ggcggctggc ctcggcacgc ggatgaagag cgatacggcc
    11821 aaggttctcc acaagattgc tggtcgaccg atgctgcact gggtggtggg agcggcgcgc
    11881 gcagctggcg ccgagcgagt ggtcgcgatc ctcggccatc aacacgaaac cgtgaaggcg
    11941 gcgctggatg cgagctttgg cgccggcagc gtcgagatcg cactgcagcc cgagcaacgt
    12001 ggcaccggtc acgccgtgca gtgcgcgttg cccgcggtga cgaacgagcc cgacgatcgc
    12061 atcgtcgtga tcctgaccgg tgacgcaccg ctgctcgtca gcgaacgcat cgccgagctc
    12121 gtgcgtgcgt gcgcggaatc gaagtctggc ctcgcgctgc tctcgacgcg gcccgatcgc
    12181 gacatgccgt acggccgcct cgtgcgcgac caaagcggcg tgctcgagcg catcgtcgag
    12241 catgccgacg ccacgccgga tcagcgtcag atccgagaca cgaacgcggg cttctatgcg
    12301 atccagctcg gccacctgcg caaagatctc gcgtcgctcc gtgcggacaa cgcgaagggc
    12361 gagctctacc tgacggactt gatcgccgcc gcgtacaagc gcggtggagc gacgtcgatc
    12421 gacgcgccgt ttaccgaagt ctcgggcatc aacgatcgcg tcgacctgtc gacggtcgaa
    12481 gcagaggcgc gccgccggat caacgagagg tggatgcggc agggcgtgac gatggtcgat
    12541 ccgcgcgcca cctacatcga cgccgacgtc gggccgctcg gcaaggacgt ctggctcggg
    12601 cccggcgtgg tgctgcgcgg caagaccaag atcggcgaca acgttcgcat cgacgcgggc
    12661 gccgtcatca cggatgtcac cgtcggggac gatacgtaca tcaaggcata ttcgatcgtg
    12721 gcggagacgg tcatgggcga acgcgtcgag gtcggtccgt tcagccactg tcggccaggt
    12781 acgcgaatcg acgaagacgc gcgcatcggt aacttcgtcg agaccaaaaa gacacacgtg
    12841 atggccggtg cgaaggccaa ccacctcgcc tatctgggtg atgcctcgat cggccagaag
    12901 tcgaacatcg gtgccggcac gatcacctgc aattacgacg gcgtgagcaa gcacaagacc
    12961 acgatcgaag cgggcgcatt catcggttcg gatagccagc tcgttgcgcc ggtcacagtc
    13021 gggcgagacg cgtacgtcgg ctccggatcg acgatcacca aggacgtccc ccgtggggcg
    13081 ctcgcgctca gccgcgtcaa acagacaaat atcgaaggct gggcggatcg gttccgcgaa
    13141 gcccaggcca aacgcaagaa gtcctccgac gacggccact gagcatgcta tttcacagcc
    13201 gtgtactgct cgatcgacaa gatcgacctc gcggcacgca tcgagggtag accggtcgcc
    13261 gtgcagaccg atcaccgcgc gtacgcggag atcgaggccg agcccgagct gtcggtgttg
    13321 tacgcgatgg cgcgcgtgct caacgcgcgc acgcaccttt tcgaatccgg tcacaccgat
    13381 gctgtcgttc actacgcggt gaatcacgaa ccacccgcgc tgctgcgcga agcgatcggc
    13441 gcggtcggcg gcacgctcga gcgcggcaag cgactcgagg tcctcggcgc tgcgaacgaa
    13501 atgctcgcga gcgagctcgc cgatcgtagc ttcgccgcgc tcgcgaatcg cgtgaagtcg
    13561 cgcgtcggca gccgcgatgt agcgatcgcg ctgcgcatgc tcgaagatca aacgttcacc
    13621 gatccgccga gccgcgacga cgagccagcg tactggcagc gcgtgctcga gctggccgca
    13681 ctcgccggcg agctattgcg cgcgaggtac ccggggcact gggtgcagac cgatcgcgcg
    13741 ctcgtgccgt tcggcttcca ggtgcagagc ggtgccgccg tgatgttccc gacgaaccgc
    13801 gcgcagcggg tgatcgaaga cggcgccgac gagagcctgt tcaagctgct gctcgccgcc
    13861 gaggaaacgg tacagcgccc gaccgatgcg atgaccggcc ggctgatgcc gagcctgcgc
    13921 agccgctcgg atatcgagct cgacgaggtc gtgtggcgcc cgatcgtcga cgatcaggga
    13981 ccgctcgact taccagttgt cgtgtgtggc atcgacggcg agagcacgtt cggcatgatc
    14041 cgccgcgagg cgatgacgaa gaccgtcgat gccgcgttcg aagaagcgct gatgaacctc
    14101 ggcgacgaag aggtgcagtg cgaagagatc gagatcgccg gcctcaccgt gatcatggtg
    14161 tcgggcaact tctacgcggc ggagaagatc ctcgatcgcg agttcatgaa gtcgttgcac
    14221 gaggagctcg actcggagct gctcgcggca tcgacgcccg cgcgcggctg cctgctcgtc
    14281 accgcgaact atcacgacaa tgcatcgctc gcgcggttcg ctgcgatcgc gcatgcacgt
    14341 cacgaagatg gcggcggtcg cgcgatctcg ccggccgtgc tgctcgtcga gaagggccgc
    14401 gtcgctggtt acgtgcgcgg ccccaatgtc cccaaagaac gccccgagac tgttactgcg
    14461 cgagcagaga ccgttccgtc gacaccgaag cgtgtaggct tgtggcgtcg tctgctcggc
    14521 cgcaagtgaa cgcagaattg aaccgccaag acgccgggaa cgccaagccg aagaaaaaga
    14581 tcttctgggc ttggcgtgtg agtcgccttg gcggtttatc tctcttgctc cttgcctgca
    14641 gcggcaaggc gaaagtgcct aagcgcgacg atgcagcggt tcatgcgagc accagcgacg
    14701 ccggaactgc ggatgccgac aaggcggtgt ttccggagct cgcgcagttg ccgaacgtcg
    14761 aacccgttcg agtgatcgcg ttgcccgcgc gtcctgatgt gccgcggttc aacgtgggcg
    14821 gtcccgtgct ggcgggcgac gtcgcggtcg tgagctcgtc gcagttcggc ttcatcgctg
    14881 tcgactttcg cggtggccac atcgcgtgga cgaagcccgc cggcgcacac gtcgcgccac
    14941 cgcagctgat cgacggcaac gtcgtgttga tcggcgactg catcaatccg cctgaagtca
    15001 aagatgacga gacgctgctc gcgtgcatgc gcgtcgtcac gccgaccggc tccgacgaag
    15061 cgtatgtcgc cgtacgcggt aaacagaaag tggtcgctgc gttcgcgggc agcgcaggtg
    15121 agcagcacgt cgacgccacg gataaaggag tgctgtggcg gcgcggcgag caggccgtcg
    15181 tcatcgatct cgtgacgggt gtcgcgacgc ccgcgccggc gaccgagcct ccgctggtcg
    15241 tcacctataa ggagaagacg tggcggatcc gtcgcaccga ggacggtgtg atcaccgcgg
    15301 aaggcaaacc gccgtggcac accgatcgcg cgtacggcac gttgctcggc ggcgtgtaca
    15361 ttccggagca gtcaccgttc gtgcgtgtcg cgagcccgac gcgccatcgc ggccagcccg
    15421 agatcctgtt gttcgacatg gacgcgaccg gttcgctcaa cagtcaggtc tcgatgagcc
    15481 cggcgcctgg cattggcctc atcggtcatg cgatcgacag agttggcgac gtcgcgctcg
    15541 cagtcaggct cgatacctca ctcgagcgcg attacatcgc gggctacgcg gcgaacgcgc
    15601 tgctcatgtg gacgtatccg ttgccgcgca tgccgcgcgc cgatccggtg ggtattgcga
    15661 tcgcgcacga tgcagtcgtc gtgttccacg acggcgacac gttcacagtt ttgcccgaat
    15721 tgtcggcgcc tcctacggcg ccaggcgcgg tccgcgagcc gtcggaaaac gctacaccgt
    15781 aagtccgcga gcccattcgc aagtcagctg gtacgccgcc tgctcttagg tcccatcgac
    15841 caacgaggag cctacgatgt ccaagctgct ttccaccgtg ctgactgcca cctgtgctct
    15901 gtcgctagcg gccaccggct gcatcaagca agacgcaccg cccgatgaaa tcgcgcgcgc
    15961 gattccgact gccgaacaag ttcgcatcaa gctgccgaac agcggcaaga cgctcggcga
    16021 tctcgcgaac tactacgtcg tcacccgcga cgtcacgcgc acgctcaacg gcggcaccgc
    16081 gtgggtgctg gtcctgatcc actcgatcgt tcagtacccg gtcacgtcga ccaacggcaa
    16141 cgtgtacacg tggggaccgt ggagcgacgc gctcgctccc gccgagtaca agctcgatgt
    16201 caccgcgaac gccgacggca cgttcgacta tcaactgtcg ggacgtaaca agacggtttc
    16261 aggatcgcag ttcgaagtcg tgatcgacgg tcacgccgac ccgcgccccg gcgacctcca
    16321 gggcaacggc gacttccagc tcgacttcga tgctggccgc cgcgtgaacc cggtcgactc
    16381 gggcgacgcg aagggcaagg tcacggtcca gtacgacctg gccgcgcgtc acctcgacat
    16441 gctcgcgcag gcgcccgacg cgaacggcaa cccggcgatg ttcgagtacg cgtacaacga
    16501 gacgctcgac ggcggcggtg acatggtgtt caacgcgacc gccgatgccg gtggtacggc
    16561 gcttcccgag aacatcgtga tccgctcgcg ctggcagggt gatggcgccg gccgtggcga
    16621 cgcccgcctc gccggtggcg acctcggcac cgcgcaggcg attgcctcgg agtgctggag
    16681 cacgctgttc cgtcgcgtgt actacaccga caacgcgacc tggcagccga ccgagggtga
    16741 cgtcgcgcag tgcgcgtacg gcacagcgga cctcccgccg ctcgactgag gcagaaactg
    16801 agtcctgctc ttacgtcctc cctccagccc acagcccttg tgggctttcg tcgtttcggg
    16861 gtaacaccct cggccggagg attttcacga tggcaacgat gcccaaggac gctcaggatc
    16921 tgttcgacaa cctcgtcccg gagggcttga agctgtaccc ggacaaagct cgcgagctca
    16981 acgcgatcta ctgcttcaag attacgggcg acggcggcgg cgagtggacg gtggactgca
    17041 ccgcggatcc gccgagctgc acgcgcggcg attcgggcaa ggcccagtgc acggttgaag
    17101 tcgcaagcga cgacttcaag acgatgctca gcgaccccaa cgcgggcatg cagctctact
    17161 tccagggcaa gctccgcgtg tcgggcgatc cgatgctcgc gatgaagctg cagcagctgt
    17221 tcgagatcgc gcgtccgaag tgaattaagc gcgcgagtca aggcatgatg tatccatgac
    17281 tggacatcgt gcctcggcac ttgccgacgc attgcgcggg attcgagttc tcgatctctc
    17341 gcgcctgctg ccgggtccgt atttgacgat ggtgctcgcc gacatggggg ccgacgtcgt
    17401 caaggtcgaa gacccgaagc tcggtgacta cctgcgcgcg ttcccgccgg gcaaaggcgg
    17461 aatgtccgga cgcttcctcg cgatcaatcg cggcaagcgc tcgctcgcgc tcgatctcaa
    17521 ggcgcccgcc gcgcgcgacg cgttcttgaa gatggtcgag aaggccgacg tcgtcgtcga
    17581 gagctttcgc ccgggcgtga tggacaagct cggtatcggc tacgccgcgc tcgccgcacg
    17641 caatccgaag atcatcgtgt gctcgatcag cggcttcggc cagaccgggc cgtacgtcga
    17701 gcgcgccggt cacgacctca attacatcgc gctcgccggc gtgctcgcga tgaccggtcc
    17761 acaggacagc gcgccgcaaa tgcccggcgt ccagatcgcc gatctcgcgg gtggcgcgct
    17821 gtggggtgcg accgcgatcc tcggcgcact cgtcggccgc gatcgcactg gcaagggcgc
    17881 gcacctcgac atctcgatga ccgagggcgc actcgcgcta ctcgccgccg aattcggcaa
    17941 tctgttctgc ggcgcgacgc catcacgcgg caccgagatg ttgaatggcg ctgccgcgtg
    18001 ctacggcgtg taccgcacga aggacgaccg gtacgtgtcg gtcggtgcgc tcgagccgaa
    18061 attctggatc gcgctgaacc aggcgatcgg ccgcgcgccg aacgtcgccg agatcgtcga
    18121 catgaagctg caggcgaaga cgcgcgagga tctcgcagcg atctttgcga cgaagacgct
    18181 cgccgagtgg actgcattct tcgcggagcg cgattgtctc gtcgagcccg tgctcgagcc
    18241 gcacgaagtc gtcgaccatc cgctgcaccg cgcgcgcgac gtgttcttcg agatcgatgg
    18301 tggcgaaggc gtcggaccga tcaagcaggt gcgcacaccc atcggcacac cggcgcacgc
    18361 gacgccacct ccgcgcctgg ggcaacagtc ccaggctgtg ctccgcgaat acggatttag
    18421 cgacgagcaa atcgctgcac tgacgacata gatcaccaaa aattttgcgg tgagagatgt
    18481 aggggcgaga tcattgcgct tcgcacgcga ctcgcgcgcg cgattacccc actatcgatt
    18541 ttgggagact ctatttatga agcacatcct gctcgctgga ttgctcgcgt tgtttctcgt
    18601 cgcgtgcggc gacaagaaat ccgacggacc gagctgcacc gcaaccggaa ccgcgtgcgc
    18661 gaacggcgca gctgactgct gcaccgggta ctgcgaggag ctgaccggca attgcgcgcg
    18721 cgcccctggc acgtgcggcg ccgacggcac cgactgctcc gtcggtcccg actgctgctc
    18781 gcttcactgc gacgacaact tcaagtgctc cggcgacgcg tgcgtctccg ataaccagga
    18841 ttgcggcggc gacaacgact gttgctccgg catctgtacg agcggcaagt gcgccccgct
    18901 gaacccgggc tgcaagacgt cgggtaacac ctgcgccggc cacagcgagt gttgctcggg
    18961 gttctgcgac aacggcacgt gcagcatctc gccctcgttc tgcacccaga acggcgacgt
    19021 ctgcgtcaac gacggcgagt gctgcggcgg tacgtgtatg aagccagacg gcgcgacgct
    19081 cggcacgtgc gcagtcgttc cttcgaatgg cgcgacgggg tgtgacacgc tcggcatcgt
    19141 ctgcgctcaa ggcgcttcgt acgacccggc aactccgctg ccgacgtgcg gtggcagttg
    19201 ctgtagccgc gcgtgcttcc cgtatggccc gagcggcgtc ttgatctgtc agccgccgag
    19261 cggttgccgg ccgaccggcg agctctgcta cgaggacgcg gactgctgcg gcggtccggg
    19321 ccagccagac aacaatccgt cgaacgtgat gtgtcgaaag gaggcgggct acgccgtcgg
    19381 gcgctgcgac aacggtaact cgtgctcgcc ggccggtgcg atctgccgcc tgcagagcgt
    19441 cgagtgcaac gcgaacgcga actgctgcgc gggtaacgtg gagcagttcg atacttgcca
    19501 tcaagacagc ctcggtatcc cgcgctgtgg cgtcggcgtg ggcatcgact gtaccaatcc
    19561 agcggaccac gccggcatgc cgtgtgcgtc gagcgcggac tgctgcggcc tgccgtgcgt
    19621 cggcaacccc gatactggct tcgtgtgcgg ctcgacgtgc gtgacccagg gcggcatgtg
    19681 caccacgacc gcggactgct gcgcgggtct gccgtgcaac gtccccgcgg gcgagacggt
    19741 cggtatgtgc ggtatgccgc aaggctgcgc cgactacggc caggcgtgca cgacgacggc
    19801 ggactgctgc aacatgctgc cgtgtaacaa cggcaagtgt gagggcatca tcctctagtc
    19861 ggtacgaccg ctacgtcggc caagcggctc ctcgatgcag gagccgcttt ttttgccctc
    19921 agtgcggcga gtgcttttcg gcgcggccga gcggctagct acggccaggc gtgcacgacg
    19981 acggcggacc ggcgcaacat gctgccgtgt aacaacggca aaaaggcatc atcctatgct
    20041 cactctacgt gataagtgag tgtgagcgaa aagccgagcc gcgagaaata caccatgtcc
    20101 cgcacgccgt cgttgttcgt gcccgtcgga aattcgaggc ggtactgcga gcggcctgcg
    20161 cgtgggcctg cgttcgtgta gtcgtctgcc acgctgcccg tcacgtcgag cgacttctgc
    20221 tcgaccgttg cattcgcaga gaaatcgccc gcggtgacca atggagcacc gccaaatgcg
    20281 gcgctgtcga acgtcatgaa tgtcacgtgc tgagccttta cgacaccaag tttcgtgtac
    20341 ggcgaacctg agaccgagtc ctgtgaaccg gtaaggagaa cgcgttcgac cgcggcgccg
    20401 gtcggaagcg tgggcaactg gaagctcacc aacagtcgca cgtacg
//
LOCUS       DASUFX010000644         9815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1908952, whole genome shotgun sequence.
ACCESSION   DASUFX010000644 DASUFX010000000
VERSION     DASUFX010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908952"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFV99_26115"
     CDS             <1..607
                     /locus_tag="VFV99_26115"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5062887.1"
                     /translation="ITGPRLHHVRDAGSQTSLSILFRAVPELDPAYVALVALLRVLDD
                     GMSTRLHYTLADQKGLAYSIHAAIEPLADAALFEITGATANAKVPSLVRELLVLLDGL
                     RKGQVSADELAKARVRYRYETLASIDDAAAMAGWFGGTALYYRPPALSERLAQIEKVS
                     VDDVVQVAEQVLAPHHLALAAVGSLSRARLGELRQTVSDWR"
     gene            653..1345
                     /locus_tag="VFV99_26120"
     CDS             653..1345
                     /locus_tag="VFV99_26120"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX5062888.1"
                     /translation="MGGIGDSYRNIVVLTGAGISQAAGLPTYRGPGGLWNDPAKIALS
                     NVEALNTRRTDACAMFWQLYSSAREVKPTAAHRALAAFEAKLPAGANFLLITQNVDGL
                     HQAAGSTRVCEFHGSLARWRCFDCGAEQTLPTEPTHCGQPMRPCAVLFGEEIPAEAEH
                     AAKRALRDCDLFVAIGTSGTVVPAASFVRWAAYAGARRVLLNLEVDDDARAEFSECHT
                     GAADELVPRWFG"
     gene            1471..2580
                     /locus_tag="VFV99_26125"
     CDS             1471..2580
                     /locus_tag="VFV99_26125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062889.1"
                     /translation="MRAPSAAEPYISASCDVTPGIFPSDGSTNVPRNAHAWVFGGTSG
                     DYHLRHGKQDVVLKGTVHDPFRIVEVPLGALEPYTTYTLAYADQVLTTFTTGASNDVE
                     PPAAPDLTWAHLSSQNGLPVDLRFEPPGDTAIMKLTVVTDGRAVSMFAPAASLQHFGA
                     ECAGASLHVNEYVCFDLAAIDLAGNRSAAARRCTTVTQVYYRHRGDDFRDRSYDPNRT
                     HWGLVIFMLVFSLVSAVNVVLFRTRTARKAFAASSLIPIAAELVRRLALLQRRNAAIV
                     FGACIVIVAISCGTGGLNAGAVLGAAPIALAVLACGDLWRTHRVLRRLHNEPDHATMH
                     DGNYYLFTADGEWIAVFPSQMELVRKAAVPTAKVR"
     gene            2695..3237
                     /locus_tag="VFV99_26130"
     CDS             2695..3237
                     /locus_tag="VFV99_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062890.1"
                     /translation="MKRSLEPQAHLEASTATQLGGNVAQGADWHGAAVAFALMGLCGF
                     AFSITRGNAVRRTFATSQWMEDADIDVMLNVAKTQATRMAMFVGVCATALALFSSLPI
                     ELEARVILSVSPAMLMTIGVVALVRLQRLAAIDPDGIRVTAHGHYLFVARDKQLVGWV
                     GASPELLARASALPTAKLHR"
     gene            3257..4222
                     /locus_tag="VFV99_26135"
     CDS             3257..4222
                     /locus_tag="VFV99_26135"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5062891.1"
                     /translation="MRRSTALLLLCGCRLNFDLVPGDDVPGDGSGGAACSRTVQVVPP
                     SGAADGVLATGGTDTAGSCGGEGIGDLLFGIDVPSGAQLMAATDGPDSAPNTVLYVRT
                     DCDDPATEIVCNRDSGIAGFSAARIEGLSAGRYYVFFDGDAGETGTVDGTIQLLLPDG
                     AACTGDATRDRCGSESHCDAVTKRCVPSACTVAEMFTTAGTYNRTVTTMPGLPSLHGA
                     TRSDCTSGGDNGVRAPEAVYRVQLAATATLRVSTASTQTDFDTLVYVRDGCTGAEMGC
                     DDDSGSSVEALTSDLTLVNLPAGDYYIFVDGFSTNTGSADLTITF"
     gene            complement(4219..5082)
                     /locus_tag="VFV99_26140"
     CDS             complement(4219..5082)
                     /locus_tag="VFV99_26140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062892.1"
                     /translation="MRRLWGTFPLILVFGCASAGDDPYGTNSTDPPVEKDPYWASGSP
                     TNEGASTHLFIVNRAITILGKHTSLPQASKAFARLNNSTCRTRWQLGLDDADHKVSYN
                     NWYTWRSHFYDPSTGTNYTGQTSPVAYNEALKHLQTAKTKLAANDVNNGCYELGLALH
                     YATDMTQPMHAANFAATDRPLDMHSHVEDYAVEIQNGYVASDWNTAPTGTVNTALSNL
                     AWAANSKWPGLWNALGNAYIAKCNDDMDDEYFDTTSCWKGDAGVNAAIGAALRDAQID
                     TAKFLYAAAIP"
     gene            complement(5216..6451)
                     /locus_tag="VFV99_26145"
     CDS             complement(5216..6451)
                     /locus_tag="VFV99_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5062893.1"
                     /translation="MLFAELGLIQPIAQAVASEGYEKPTPIQARAIPHVLAGRDLLGL
                     AQTGTGKTAAFALPILQRLAHIAPGRGHRHIRCLVLTPTRELASQIGESFSTYGRHLP
                     LRTTVIFGGVGEAAQKQALKQGIDILVATPGRLLDLSGQGFVDLRQLEVFVLDEADRM
                     LDMGFIHDVKRVIAMLPSRRQTLFFSATMPPEAQDLADRLLIKPETVAVTPPSTTAEK
                     VEQEVFFVEKGDKRALLVDVLRDSAMRRVLVFSRTKHGANRIAEHLSKASIGAEAIHG
                     NKSQNARERALANFKAGRIRVLVATDIAARGIDIDDITHVVNFDVPEVPETYVHRIGR
                     TARAGATGMALTFVEVEERADWKNILKLTRQDIPVVEGHPYESRVPFAVTPPSQGPTR
                     APRPHNPQGHRRGRNRRRR"
     gene            complement(6500..7633)
                     /locus_tag="VFV99_26150"
     CDS             complement(6500..7633)
                     /locus_tag="VFV99_26150"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5062894.1"
                     /translation="MFDRQRTVTTGITTAAVATVALVLWLKPWATEALVSEQPKPPPI
                     AQSGHPVDIVFAVDTTGSMGGLLDGAKRTVWSIANQVKSIDPNADLRVGLVAYRDLGD
                     DYVTKDFSLTDDLDAMYVELSSYQAAGGGDVPENVDAALYDAVHKMKWRTGAKKMIFL
                     VGDAPPATRGEVPTYEQTAQQAAKMQIKINAIRCGQDGDTARAWQQVAMLGNGEFSTI
                     QQDGGVQQIATPYDDRMAALAGEIDSTTVVYGDAAVHRGYDAKIAAAEAAPAAAKADR
                     GVYYAKKGATSTKPTEDAVAGVATGAMNVDELEEGMLPTEMRNKSKAELKAELEARAK
                     KREVAQKEMADLAKQRDEYLEKNAKDKNGFDGVVKKTLEAQLK"
     gene            complement(7707..8186)
                     /locus_tag="VFV99_26155"
     CDS             complement(7707..8186)
                     /locus_tag="VFV99_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062895.1"
                     /translation="MRIALVAAVLLFARADAQNVGWDLRIPERVELVPGASGTLPITI
                     NVDRGRTISKDAGLTIDLAPEGGVSVKKRRLGRGDAVDPEADAPRFAVPLRADAAGDF
                     AVKVHVRFWLCGSKVCRPIEARRSVAVAVTTPTPPPAPPVDAGIDAPRPDAGRRKHK"
     gene            complement(8183..9490)
                     /locus_tag="VFV99_26160"
     CDS             complement(8183..9490)
                     /locus_tag="VFV99_26160"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5062896.1"
                     /translation="MAVKNYVLDTNVLLHDARAVYAFADNNVVIPIYVLEEVDTFKKD
                     QSELGRNARQVARILDEIGHKGSLQHPQPLPSAGTVRVALSKNPPKNPSYDSRSMDQR
                     ILELALEVRDTDPKTPTIMVTKDVNMRVRGDALGLQTADFEPERISIDELYPGNRELL
                     VPSGVIDEFYANSAVVVDAPGLHANEFLTLKDEGGKSALTRWDKGVGKAVPVKKLREG
                     VWGIKPRNREQHFALDLLLNDDIKLVTLVGKAGTGKTLLAIAAGLQKVTEEQVFSKML
                     VSRPIFPLGRDIGYLPGDIEEKLNPWMQPIYDNLELLLGLNKTDKKDGRSYAELVDLG
                     FVEIEPLTYIRGRSLPNVYMIVDEAQNLTPHEVKTIITRAGEGTKIILTGDPYQIDHP
                     YLDSSNNGLTTVAERFKNEAIAGHVILTKGERSPLAELATQIL"
     gene            complement(9503..>9815)
                     /locus_tag="VFV99_26165"
     CDS             complement(9503..>9815)
                     /locus_tag="VFV99_26165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062897.1"
                     /translation="LGGLGIASLVTGVALGISAKNKYNAEFDNGHCMQDGGVSVCNDD
                     GLRNQSDAITRANIGTGFAIGGAVLATAGAIVFLTAPRDTVVIPTASAGAGGLAVVGR
                     F"
BASE COUNT         1540 a         3249 c         3227 g         1799 t
ORIGIN      
        1 aatcacgggc ccgcgcctac accacgtgcg cgatgccggc tcacaaacct cgctctccat
       61 cctgttccgc gcggtacccg agctcgatcc cgcgtacgtc gcactcgtcg ccctgctccg
      121 cgtgctcgac gacggcatgt cgacgcgcct gcactacacg ctcgccgacc aaaaaggcct
      181 cgcctactcg atccacgccg cgatcgagcc gctcgccgac gccgcgctgt tcgagatcac
      241 gggcgcgacg gcgaacgcaa aagtgccgtc gctcgtgcgc gagctgctcg tgctactcga
      301 cggcttacgc aagggtcagg tttccgccga cgagctcgcg aaggcgcgcg tccgctatcg
      361 ctacgagacg ctcgcgtcga tcgacgacgc cgccgcgatg gccggctggt tcggcggcac
      421 cgcgctctac taccggccgc ccgcgctgtc agaacgcctc gcgcagatcg agaaggtctc
      481 cgtcgacgac gtcgtgcagg tcgccgagca agtgctcgcg ccgcaccacc tcgcgctcgc
      541 tgcggtcggg tcgctgtcgc gtgcgcgcct cggcgagctc cgccagaccg tctccgactg
      601 gcgctaaccc cgtcgactgg cgccagactt attgtctgct atacacgaag ccatgggtgg
      661 catcggggac tcgtatcgca acatcgtcgt gctgaccggc gccggcatct cgcaggccgc
      721 gggtttgccg acgtatcgcg gcccgggcgg cctgtggaac gacccggcga agatcgcgct
      781 gtcgaacgtc gaggcactga acacgcggcg caccgacgcg tgcgcgatgt tctggcagct
      841 ctactcctcg gcacgtgagg tcaagccgac cgccgcacac cgcgcactcg ccgcgttcga
      901 agcaaagctg cccgcgggcg caaacttcct gctcatcacg cagaacgtcg acggtcttca
      961 tcaagccgcc ggctcgacgc gcgtctgcga atttcacggc agcctcgcgc gctggcgctg
     1021 cttcgactgc ggcgcggagc aaacgctgcc gaccgagccg acgcattgcg gtcaaccgat
     1081 gcgcccgtgc gcggtgctgt tcggcgaaga aatcccggcc gaggccgagc acgcggcgaa
     1141 gcgtgcgttg cgcgattgtg atctcttcgt cgccatcggc acgtcgggca ctgtcgtacc
     1201 cgcggcgtcg ttcgtgcgct gggccgcata cgccggcgcc cgtcgtgtgc tgctcaatct
     1261 cgaagtcgac gacgacgcgc gggccgagtt ctccgagtgc cacaccggcg ccgccgacga
     1321 gctcgtgccg cgctggttcg gctagcgact gcgtacgaat tcgcgtcgca acccaagccg
     1381 cttcgggcaa gatccgtcca acgcaccatg cgcgcactca ttgtcgtctt attgctcgcc
     1441 acgactgcga gcacagcgag tgctttcaag atgcgcgcgc ccagcgcggc ggagccgtac
     1501 atctcggcgt catgcgacgt gacgccggga atctttccga gcgacggatc gacgaacgtg
     1561 ccgcgcaacg cgcacgcgtg ggtgttcggc ggtacctccg gcgactacca ccttcgtcac
     1621 ggcaagcagg acgtggtgct caaaggcacg gtccacgatc cgtttcgcat cgtcgaggtg
     1681 ccactcggcg cgctcgagcc ctacacgacg tacacgctcg cgtacgcgga ccaggtactg
     1741 accacgttca cgaccggcgc gagcaacgat gtcgagccgc ccgcagcgcc ggacctcacg
     1801 tgggcgcacc tctcgtcgca gaacgggctg ccggtcgatc tgagattcga gccaccaggc
     1861 gacaccgcga tcatgaagct caccgtggtc accgacgggc gcgcagtctc gatgttcgca
     1921 ccagcggcct cgctacagca cttcggtgcc gagtgcgcag gcgcctcact ccacgtgaac
     1981 gagtacgttt gcttcgacct cgctgcgatc gatctcgcag gcaaccgtag cgcggcggca
     2041 cgtcgctgca ccaccgtcac gcaggtgtac taccggcacc gcggcgatga cttccgcgac
     2101 cgctcctatg atcccaacag gacccactgg gggcttgtca tcttcatgct cgtattcagc
     2161 ctggtctcgg ccgtcaacgt ggtcttgttc cgcacgcgaa ccgcaaggaa ggcgttcgcg
     2221 gccagctcgc tcataccgat cgcggccgag ctcgtgcgcc gcctcgcgct cttgcaacgc
     2281 cggaacgcgg cgatcgtgtt cggagcgtgc atcgtgatcg tggcgatttc ctgcggaacc
     2341 ggcgggctca atgcaggggc tgtgttaggt gccgcaccga tcgcgttggc agtgctggcg
     2401 tgcggagacc tgtggcgaac gcaccgcgtg ctccgccgcc tccacaacga gcctgatcac
     2461 gcgacgatgc acgacggcaa ctattacctc ttcacagcgg atggcgagtg gatcgccgtc
     2521 ttcccgtcgc agatggagct cgtgcgaaaa gctgcagtgc ccacggcgaa agttcggtag
     2581 catgagtaca tgatgggccg tgctgtcatc gtgctctgtc tgctggtggg tgtagcgaac
     2641 gcgtgtccga tcagattcga ctcaccggaa caagagcgcg ccttcctcga gcgcatgaag
     2701 cgttcgctgg aaccacaggc tcacctcgag gcgtcgaccg cgacgcagct cggtggcaac
     2761 gtcgcgcaag gcgcagactg gcacggtgcc gcggtcgcgt tcgcgttgat gggcctgtgt
     2821 ggcttcgcat tctcgatcac gcgcggcaat gccgtgcgcc gcaccttcgc gacgagccag
     2881 tggatggagg acgccgacat cgacgtgatg ctgaacgtcg ccaagactca ggcgacccgc
     2941 atggcgatgt tcgtcggcgt gtgtgcgacc gcgctcgcgc tgttctcgtc gctaccgatc
     3001 gagctcgagg cgcgcgtgat cctctcggtc tcgcccgcga tgctgatgac gatcggcgtg
     3061 gtcgcgctcg ttcggctgca gcggctggcg gcgatcgatc ccgacggcat ccgcgtgacc
     3121 gcgcacggtc actacttgtt cgtcgcgcgt gacaagcagc tcgtcggctg ggtcggagcg
     3181 tcacccgagc tgctcgctcg tgcgagcgcg ctaccgaccg cgaagctgca ccgctagcgc
     3241 gtgtacacta aagaacatgc gccggtcgac cgcgttgctg ctgctgtgcg gctgccggct
     3301 gaactttgac ctcgtaccgg gcgacgatgt acccggcgac ggctccggcg gagcggcgtg
     3361 ttcacgcacc gtgcaggtcg tgccgccgtc gggcgctgcc gatggggtgc tcgccaccgg
     3421 cggcaccgac actgcgggct cgtgtggcgg tgaggggatt ggcgatctgt tgtttggcat
     3481 cgacgtgccg agcggcgcgc aactcatggc tgcgaccgat gggccggact cggccccgaa
     3541 caccgtgctg tacgtgcgaa ccgattgcga cgatcccgcg accgagatcg tgtgcaatcg
     3601 cgacagcggc atcgccggtt tctctgcggc acgcatcgag ggcctcagcg caggccgcta
     3661 ctacgtgttc ttcgacggcg acgccggcga gactggcacc gtcgacggca cgatccagtt
     3721 gctgttgccg gacggcgccg cgtgcaccgg tgacgcgaca cgcgatcgct gcggctcgga
     3781 gtcgcattgc gatgcggtca caaaacgctg cgtgccgtcc gcatgcacgg tcgcggagat
     3841 gttcacgacc gccggcacgt acaaccgcac cgtgaccacg atgcctgggt tgccgagctt
     3901 gcatggcgcc acccgaagtg actgcaccag cggcggcgac aacggcgttc gcgcaccgga
     3961 ggctgtgtat cgtgtccagc tcgccgcgac cgcgactctg cgcgtatcga cggcatcgac
     4021 gcagacggat ttcgacacgc tcgtctacgt gcgtgacggc tgcaccggtg ccgagatggg
     4081 ttgcgacgac gacagcggca gctcggtcga ggccctgacg tcggacctga cgctcgtcaa
     4141 cctgccggcc ggtgactact acatcttcgt cgatggcttc tcgacgaaca ccgggtccgc
     4201 ggatctcacg atcacgttct aaggaatggc ggccgcgtag aggaacttcg cggtgtcgat
     4261 ctgggcgtcg cgcagcgctg caccgatcgc ggcattgacg ccagcgtcgc ctttccagca
     4321 cgacgtcgtg tcgaagtact cgtcgtccat gtcgtcgttg cacttcgcga tgtacgcgtt
     4381 gccgagcgcg ttccacaggc ccggccattt gctgtttgcg gcccacgcaa ggttggacag
     4441 cgcggtgttg acggtgccag tcggcgcggt gttccagtcg gacgcgacgt agccgttctg
     4501 gatctcgacc gcgtaatcct cgacgtgcga gtgcatgtcg agcggccgat cggtcgcggc
     4561 gaagttcgca gcgtgcatgg gctgcgtcat gtcggtcgcg tagtgcagcg cgaggccgag
     4621 ctcgtagcag ccgttgttga cgtcgttcgc ggcgagcttg gtcttcgcgg tctgcagatg
     4681 cttgagtgct tcgttgtatg ccaccgggct ggtctggccg gtgtagttcg tcccggtgga
     4741 cggatcgtag aagtgcgatc gccacgtgta ccagttgttg tacgagacct tgtgatcggc
     4801 atcgtcgagg ccgagctgcc agcgcgtgcg gcacgtcgag ttgttcagcc gtgcgaacgc
     4861 cttcgacgcc tgcggcagcg acgtgtgctt gccgaggatc gtgatcgcgc gattcacgat
     4921 gaacaggtgc gtgctcgcgc cttcgttcgt cggactaccg gacgcccagt acggatcctt
     4981 ttcgacaggc ggatcggtgc tgttcgtgcc gtacggatca tcgcctgcgc tcgcgcagcc
     5041 gaaaactaga atgagcggga acgtccccca cagtcgcctc atgtggcatc ggtatcaccg
     5101 ttgcgtgacg ggcccgcgat gaggttgctt tcatcacctc gctcgcacgc ggattgattg
     5161 cgcacaacag tgcgcatccg cgatcccgcg caacgcgtgc gcgcgaaagg agctgttatc
     5221 gccgacgacg attgcggccg cggcgatggc cctgcggatt atgcgggcgg ggcgcacgcg
     5281 tcgggccttg gctcggcggc gtcactgcaa aaggcacgcg tgattcgtag gggtggccct
     5341 cgacgaccgg aatgtcctgg cgcgtgagct tgaggatgtt cttccagtcc gcgcgttcct
     5401 cgacttcgac aaatgtcagc gccatgccgg tggcaccggc gcgcgcggta cggccgatgc
     5461 gatgcacgta cgtctcgggg acctcgggaa cgtcgaaatt cacgacgtgc gtgatgtcgt
     5521 cgatgtcgat gccacgcgct gcgatgtccg tcgcaaccag cacgcgaatg cggccggctt
     5581 tgaaatttgc gagagcccgc tcacgtgcgt tctgcgattt gttgccgtgg atcgcttcgg
     5641 caccgatgct cgccttcgac aggtgctcgg cgatgcggtt cgcgccgtgc ttggtgcgcg
     5701 agaacacgag cacgcggcgc atcgcggagt cgcgcagcac gtcgacgagc agcgcgcgct
     5761 tgtcgccctt ctcgacgaag aacacttcct gctcgacctt ctcggccgtg gtcgacggcg
     5821 gcgtgaccgc gaccgtctcc ggcttgatga gcaggcgatc ggctaggtct tgcgcttcgg
     5881 gcggcatcgt cgccgagaag aacagcgtct ggcggcgcga cggcagcatg gcgatcacgc
     5941 gcttcacgtc atggatgaaa cccatgtcga gcatgcgatc agcttcgtcg agcacgaaca
     6001 cttcgagctg gcgcagatcg acgaagcctt ggcccgacag atcgagcagg cgcccgggcg
     6061 tcgcgacgag gatgtcgatg ccctgcttga gcgcctgctt ttgcgcggcc tcgccgacgc
     6121 caccgaagat cacggtcgtg cgcagcggca ggtgcctgcc gtacgtcgag aagctctcgc
     6181 cgatctgcga cgcgagctcg cgcgtcggcg tcagcacgag gcagcgaatg tggcggtggc
     6241 cgcgccccgg cgcgatgtgc gccaggcgct gcaggatagg cagcgcgaac gccgcggtct
     6301 tgccagtgcc ggtctgcgcg agcccgagta ggtcgcggcc ggcgagcacg tggggaatcg
     6361 cgcgggcttg aatcggtgtc ggtttctcgt agccctcaga cgcgaccgct tgtgcgatcg
     6421 gctggatgag cccgagctct gcaaacaaca tagacggggc gatatagcag tgcccggccc
     6481 cgatggatag aaaagatagc tacttgagct gggcctcgag ggtcttcttg accacgccgt
     6541 cgaacccgtt cttgtccttc gcgtttttct ccaggtactc gtcgcgctgc ttggccaagt
     6601 cggccatttc cttctgggcg acctcacgct tcttcgcacg ggcctcgagc tcggccttga
     6661 gctctgcctt cgacttgttg cgcatctcgg tgggcagcat gccctcctcg agctcgtcga
     6721 cgttcatcgc gccggtcgcg acacctgcga cagcgtcctc ggtcggcttc gtcgaggtcg
     6781 cacccttctt cgcgtagtag acgccacggt cggccttcgc cgctgccggc gcagcttccg
     6841 cggccgcgat cttcgcatcg tagccgcggt gcactgccgc gtcgccgtag acgacggtcg
     6901 tcgagtcgat ctcgcccgcg agcgccgcca tgcgatcgtc gtacggcgtc gcgatctgct
     6961 gcacgccacc gtcctgctgg atcgtcgaga actcgccgtt gccgagcatc gcgacctgct
     7021 gccacgcgcg agcggtgtca ccgtcctggc cgcagcgaat cgcgttgatc ttgatctgca
     7081 tcttcgccgc ctgctgagcg gtctgctcgt aggtcggcac ttcgccgcgc gtcgccggcg
     7141 gtgcatcacc gacgaggaag atcatcttct tcgcaccggt gcgccacttc atcttgtgaa
     7201 ccgcatcgta gagcgcggca tcgacgttct ccggcacgtc gccgccgccc gcggcctggt
     7261 agctcgacag ctcgacgtac atcgcgtcga gatcgtcggt cagcgagaag tccttcgtga
     7321 cgtagtcgtc gccgaggtcg cgatacgcga cgagaccgac gcggagatcc gcgttcggat
     7381 cgatcgactt cacctgattg gcgatcgacc acacggtgcg cttcgcgcca tcgagcaaac
     7441 cgcccatcga gccggtcgta tcgaccgcga acacgatgtc gaccggatga ccgctctgtg
     7501 cgatcggcgg cggcttcggt tgctcggaga cgagggcctc ggtcgcccat ggcttgagcc
     7561 agaggacgag ggcgacggtg gcgacggcgg cggtggtgat tccggtggtt acggtgcgct
     7621 gacggtcgaa catgcctgct ttcgacgcac cacccggcac tcggatctac gcgaaataag
     7681 gacccaaggc ttcgattcct acggtgtcac ttgtgcttac gccggcccgc gtcgggccgc
     7741 ggggcgtcaa tgccggcatc gacgggcgga gccggtggcg gcgttggtgt ggtaaccgcg
     7801 accgccacgc tacgccgcgc ttcgatcggc cggcacactt tgctgccgca cagccagaac
     7861 cgaacgtgca ccttgaccgc gaaatcgccg gccgcatccg cgcgcaatgg gaccgcgaac
     7921 cgcggcgcat cggcttccgg atcaaccgca tcgccccgac caagccgccg cttcttcacg
     7981 ctgacgccgc cctcgggggc aagatcgatc gtaaggccgg cgtccttcga gatcgtgcga
     8041 ccgcgatcga cattgatcgt gatcggcagc gtgccgctcg caccgggcac gagctcgacg
     8101 cgctcgggga tgcgcaggtc ccaaccgacg ttctgcgcgt ccgcacgcgc gaacagcaga
     8161 acggcagcga cgagcgcgat cctcacagga tctgagtcgc gagctccgcg agcggcgagc
     8221 gctcgccctt ggtcaggatc acgtggcctg cgatcgcctc gttcttgaag cgctcggcaa
     8281 cggtagtgag accgttgttc gacgagtcga ggtacgggtg atcgatctga tacgggtcgc
     8341 cggtcaggat gatcttcgtg ccttcgccag cgcgcgtgat gatcgtcttc acctcgtgcg
     8401 gcgtgaggtt ctgcgcctcg tcgacgatca tgtacacgtt cggaagcgag cggccgcgga
     8461 tgtacgtgag cggctcgatc tcgacgaagc cgaggtcgac gagctccgcg tagctccggc
     8521 cgtctttctt gtcggtcttg ttgaggccga ggagcaactc gaggttgtcg tagatcggct
     8581 gcatccacgg gttcagcttc tcttcgatgt cgcccggcag atagccgatg tcgcgaccga
     8641 gcgggaagat cgggcgcgag acgagcatct tcgagaacac ctgctcctcg gtcaccttct
     8701 gaagaccggc ggcgatcgcg agcagcgtct taccggtacc cgccttgccg acgagcgtga
     8761 cgagcttgat gtcgtcgttg agcaagagat cgagtgcgaa gtgctgctcg cggttacgcg
     8821 gcttgatgcc ccacacgccc tcgcgcagct tcttgaccgg caccgccttg ccgacgccct
     8881 tgtcccagcg ggtgagcgcg gacttgccgc cctcgtcctt gagcgtgagg aactcgttcg
     8941 cgtggaggcc gggcgcgtcg acgacgactg cgctgttcgc gtagaactcg tcgatcacgc
     9001 cggacggcac gagcaactcg cggttgccgg gatacagctc gtcgatcgag atccgctcgg
     9061 gctcgaaatc cgcggtctgc aggccgagcg cgtcgccgcg gacgcgcatg ttgacgtcct
     9121 tggtgaccat gatggtcggc gtcttcggat cggtgtcgcg aacctcgagt gcaagctcga
     9181 ggatgcgctg atccatcgag cgcgagtcgt agctcgggtt cttcggcgga ttcttcgaca
     9241 gcgcgacgcg caccgttccg gcgctcggca gcggttgcgg atgttgcagc gagcccttat
     9301 ggccgatttc gtcgaggatg cgtgcgacct ggcgagcgtt gcgaccgagc tccgactgat
     9361 ccttcttgaa cgtgtcgact tcttcgagca cgtagatcgg gatcacgacg ttgttgtccg
     9421 caaacgcgta gacggcacgc gcgtcgtgaa gcaggacgtt cgtgtcgagg acgtagttct
     9481 tgaccgccac gtcgcgacag tctcagaacc gcccgacgac ggcaaggcca ccggcccccg
     9541 cgctcgcagt cgggatcacg acggtgtcgc gcggtgccgt gagaaatacg atggcgcccg
     9601 ccgtggccag gaccgcaccg ccgatggcaa agccggtccc gatgttcgcg cgcgtgatgg
     9661 catcgctctg gtttctcagg ccgtcatcgt tgcagaccga cacaccaccg tcctgcatgc
     9721 agtgtccgtt gtcgaattct gcgttgtatt tgttcttcgc actgatgccg agcgccacgc
     9781 cggtgaccag gctggcgatg ccgagcccgc ccagc
//
LOCUS       DASUFX010000645        25811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1633255, whole genome shotgun sequence.
ACCESSION   DASUFX010000645 DASUFX010000000
VERSION     DASUFX010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633255"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /locus_tag="VFV99_26170"
     CDS             <1..1918
                     /locus_tag="VFV99_26170"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5062898.1"
                     /translation="ALRRIAWLGVVVLMFVLVLVAGGNASVIKAATSYTGLGAPIARG
                     VRKVFDRDHDGFSRVLGGGDCNDSDPKIHPGAPEIPDNGIDENCVGGDATAKQPPHDV
                     GFAPVPAAVPRDANIVLITIDTARADHFGMYGYKRPTSPTLDAIGAQGTVFEAGWAHA
                     PSTRYSMPAILTGRLPLDVYYNYAIEGWPGLSPKATTLCEALAPLGFATGAITNYEYF
                     DRYRGMDQGCAEYDNENARLHNRVTGSGPEQTKGSSSREQTDKAIAFVDRHANQKWFL
                     WVHYYDLHYSYEPHPGTSFGFDREALYDGEIRFTDSHIARLVEALRQRGLYDKTIIVV
                     TGDHGEGFGEHNVLMHGYHLYSPQTKVPFMIRVPGLPARRSTTPAGHVDIMPTLVNLA
                     GGQPNPDMEGTSLVPALAGADQPRTLFQQLSYENNHEMRGGVDAHCHVIYNVSPETSW
                     EVYRVDRDPLETEDVSGDSDQCADTRRAFERWYDAGQVPPGAAEALLPTRPAMAKPLD
                     ADLGDSVRLLGVDVPAQAKPGDAVNVTWTFEARGKVPADWKLFVHVKGPGNIFINGDH
                     KPTRPFEWWTAGQFIQYSTTITLPRFAQPGAYTIWVGMFRGNQRATVRAPHARVDNNA
                     VEAATFQVAPQVVP"
     gene            1927..3957
                     /locus_tag="VFV99_26175"
     CDS             1927..3957
                     /locus_tag="VFV99_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062899.1"
                     /translation="MRRSLEITGWALLALPALYQILLLCSAIGGRVAYPYDLEWMEGG
                     MLHHALRFQTGHGIYSEPSVEFIPYLYTPLYPALLAILGEIFGLTYTLGRVISVLSLV
                     GIAIVGAISICSPRNRNAAMLPAVVGTVLALGLFAAVYPFVEGWYDLVRADALFLFLV
                     TAGLVAAMYWTDQGEGWRGHGRVAAVGAILAFAFFTKQTGIFYVLLGGAIVLILRWRR
                     LAAYIAAAGVVGLGGSGLMNLSSGGWFWTYIRKIHSAHDFNYDRFWVSFGNILWHFRA
                     LTIVIIVTLIVVGYTWLRRPAEGSRRELPRQTRPFLLWTSAFVVSTIVGAIGWGTEFA
                     HFNAYMPAFLHGALAAGAAVPALAACAGILWGARQHSQLVVHGAAGLAALLLAVTCLH
                     WTWQPKRFKPTDADVAAGDRLIKRIKALDGEVWMPSHPWYLVLAGKQPHVHRMGVKDV
                     TTRQNRRIVGLDDALEKHRFSSIVFDNRDLFLELPRIRQFYRPALKLPADERPRLFTG
                     AMIVPDSIWVPALEVKPPAGARVVFDFEMTSWSGWTKAGSAWGPGPVTESLPGQDLVI
                     GATGQRFADSMHGGDVATGRLTSPTFPLDGNRLTLQVGGGTDATKLRIELWVEGQIVA
                     SSGAPSLGGDVLREVSWDISAQRGKFATLVLVDDATTADGHIVIDDVWIWDR"
     gene            4040..5608
                     /locus_tag="VFV99_26180"
     CDS             4040..5608
                     /locus_tag="VFV99_26180"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5062900.1"
                     /translation="MRRPISYRAGIALGAMCTLMFVAVAAVAAALFGIPNPAYRLFEL
                     VSGALPGVVVTSAIDIVVKTVHSAGATSTAAAAKEIERAMAIALATGLGGVLGAVVVW
                     FVRRGKHALLTSLVVGTVAGCVYVVIAGISAIMIVVSLAGFVAWGGVLGVSIRAMAEH
                     AGVSAERRQVLVVIGVATAATCAVAFGLVRLLGRGGARISRMLRAEDEAALTSGPAAS
                     PSPESLTARFSPVDGTRAEITDNAHFYRIDINLKPPSIDGATWTLPIEGLVGKPRVLT
                     LDELRRRPAVSQIITLECISNPVGGDLIGTSRWTGIRLADLLAELEVASTARALSFEA
                     ADGFHETCSLDDARDPRALLVYAMNGEPLTEVHGYPLRLYLPDRHGMKLPKWITRMEL
                     VADPRPGYWVERGWSAAAIPHTTSVIDAVTATGDGAERMMSIGGIAYAGARGIQRVEV
                     QIDDGAWQDAQLRTPALSPLTWVQWRLDVPYRKGKHTIRVRATDGTGALQDTVVRPPH
                     PDGATGIYTKRVDV"
     gene            complement(5605..6936)
                     /locus_tag="VFV99_26185"
     CDS             complement(5605..6936)
                     /locus_tag="VFV99_26185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062901.1"
                     /translation="MRLYAALFAIGLVVYGALAGSRIAKQSQAPQFVFQADAWLHGQA
                     HVDPPLVDNDWAKLETVELADGRIVEGKRMISKPVFRTLDGEQVPANQVRGVRGTTWY
                     MSFPPLPTLLMMPSALISGRDGNDVIPTLLVAALILPLTLLLLRRLAEAGFSQRTVRE
                     DLWLVGTLAFGSVMFFSAVQGKVWYTAHVVGVVLALLYAWASIEAKRPIIAGLALGAA
                     ALTRTPMAFMFPLFMLEAWRMAQGDRRAFVRTLVKFAIPVAAFAIAGMIYNYVRFGSP
                     TEFGHTYLEVRQAPQIEQWGLMSYHYLSRNLTVGFTLLPELPGRAPWIQIGGHGLALW
                     FTTPVLLYLLWPREKPALHRALWITVACVALPSLLYQNSGWVQFGYRFSLDYMVFLIM
                     LLAIGARPLTRLAKGLMIAGVVINLFGAWTFDREWKYYRVGGDAYDVIIRH"
     gene            7028..7822
                     /locus_tag="VFV99_26190"
     CDS             7028..7822
                     /locus_tag="VFV99_26190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062902.1"
                     /translation="MSTRPSPIAAVPALLLVVVALWETCATPRDAAEVPGDKAWAKAA
                     DVVRAGYQPGDLIVFAPDWIDPVGRLHLGDLIPVEMAARMDAARYGRIWELSIRGAHA
                     ADTAGLTATSEQTVAGITVRLFSRTPATVVSDVRDALASVKVEGAARPTLELTEVGFA
                     PHRCIQIVPPPKGTVRITFPQLALGSELVGYAGLADIFTRRSPRGPGKLAVEIGGKVV
                     ARIAPGVDDGWVRFAAPTTPGPADVTFIASADDPNRLICFAAEARR"
     gene            7819..9480
                     /locus_tag="VFV99_26195"
     CDS             7819..9480
                     /locus_tag="VFV99_26195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5062903.1"
                     /translation="MKINWKWRPSKQRIVGWVLALFTVLLVTTNQQDIGIARDETVYF
                     GAGTRYAEWWLGLVTFKHGIAKKSITDTFGGAPGGGTNSEHPPLIKTAMGLSHWLLYD
                     KLHVTDELTAYRFPGALFAGLLVLLVYSMTLALWGFPEAVIAALCTLLLPRALFHAGL
                     ACFDAPIMTLWFATIYAYWRCLDERKWPWQVGVVFGLALATKHNAVLLPFALGLHYVV
                     IAWRAGKWRGLVYHKPRVVLSLALLGPLTLIVLWPWLWFDTFAHIKQWLSFHTSHVHY
                     NFEYLGTNWNAPRFPWHVALVTTLFTVPVATLAAACAGAGVLVVRWRRRENADHERAP
                     ALLLALSAAASIGPFFLGSTPIFGAEKHWMPALPTLCIAAGVGAAWAARQAAEYVRAL
                     RPVGEVLARRVALGIVGGAIVLACATETVAAQPYALTWYNALAGEAPGGADLGMNRQF
                     WGVAARGVLPFIANQFPTRVYSHDASPAWGWYHRLGLLRGFPDAGQEGVGVERSNLAL
                     VIHEKHFNRHDYMIWKSYGTVRPVFVLRSEGVPIVTVYKRPVPRP"
     gene            9496..11268
                     /locus_tag="VFV99_26200"
     CDS             9496..11268
                     /locus_tag="VFV99_26200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062904.1"
                     /translation="MSDPVDPLTPTQAATESGEPATLGTAPVERVSVRDIPTYELGEV
                     IGRGGMGEVIAARDRTFGREVALKRMRADSPSPEHVERFLREARIQARLDHPAIAPVH
                     ELGYDSDGLPYFTMKRVGGATLAALLEAKTEKPQRLLRALVDVCHAAELAHSRRIVHR
                     DLKPSNIMLGDYGEVYVLDWGVARVMTGDRAQTSPSTDDVPSLDGVTKAGALLGTPGY
                     MAPEQVRGEAVGPAADVYSLGSMLFEILAGEPLHPRNDAIASTMTNVSRSPSQRNPAR
                     AVAPELDTICAAALATDPNLRPSAGAIATAIQDYLDGDRDVERRRELAGKLASSARAA
                     LEKGDSAHAMRSAGRALAFDPTSSEATAIVTQLVLAGPSTTETPDEVDAALEKEEQRL
                     MRQRSKRAVLPYGAIFSTLPLVPFLHVQSYPMLAAVYVAFALMVGITWLNSRVGVPIL
                     LTLTGHAITALLFSRLAGPFIITPIMLCAILLSATSIPWLNRRPLAILVWTCGTVMLP
                     FVLEWAGVLAPSWAMTSDGILSTGNVFVQSQMPASLGIAGNVAAICLVAFYARGIGRD
                     RREAQRRLFLQAWHLRHLLPKPAE"
     gene            11297..12187
                     /locus_tag="VFV99_26205"
     CDS             11297..12187
                     /locus_tag="VFV99_26205"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX5062905.1"
                     /translation="MPRLNSLTGFVFILILLAGFGLAYASYGTLANPRSTVIILLALI
                     VAGFVARAICIAAAWQRVVVLRLGKFRALRGPGLFGIIPIIDSIPYTIDIRVITSSFK
                     AENTLTRDTVPVNVDAVLFWKVIDPQRAALAVADYESAIRWAAQTALRDVIGKTMLAD
                     MLEGRGKISLELQEMIDSRTEPWGVNVIAVELRDVLIPSGLQDAMSMQAQAERERQAR
                     VILGDSERQIAEKFQEAARSYANDPTALHLRAMNMLYEGLKQNATIVLVPSTAVETMQ
                     LGNVAGITALAELGKQPTSH"
     gene            complement(12192..12488)
                     /locus_tag="VFV99_26210"
     CDS             complement(12192..12488)
                     /locus_tag="VFV99_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062906.1"
                     /translation="MTATERITMVVLNEDGSRDKFTLSSWVGIPRKHELMWIGDDPYM
                     IIEVEYAVSEGFFGARSIDAAGVYVRRLTKDEQDAVARRLANPVRGKDEQPFRP"
     gene            complement(12485..13225)
                     /gene="dapB"
                     /locus_tag="VFV99_26215"
     CDS             complement(12485..13225)
                     /gene="dapB"
                     /locus_tag="VFV99_26215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5062907.1"
                     /translation="MIDVCVLGPSGKMGRSVIEAAKAREDVRVVSTVDLGEDVAAGLA
                     KAQVYVDFTSPTGTRAAAKAALAKKTAAVIGTTGLGESEERAIAELAKVAPVVVAANF
                     SLGVNLVLGLVKQAAKVLGPDWDAEIVETHHKMKRDAPSGTALAIARAIAAGHGVDYD
                     KVKRHTRDGDVGPRPRGEIGVSTVRGGDIIGEHVATFFGAAERVEIAHRATSRTIFAA
                     GALTAAAWVVGKAPGRYDMLDVLGFAGE"
     gene            complement(13222..14109)
                     /gene="dapA"
                     /locus_tag="VFV99_26220"
     CDS             complement(13222..14109)
                     /gene="dapA"
                     /locus_tag="VFV99_26220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5062908.1"
                     /translation="MAKLPTIEGCLTALVTPFKNGKVDFEGLAKLVDWQIENGVDGIV
                     SVGTTGESATLDVDEHVAVITATVKAAKGRVPVIAGAGGNATSEALTLTKASEEAGAD
                     ALLHVTPYYNRPNQEGLFRHFEAIAKATKLPIILYNVPSRTACDMQCETVMRLADFDN
                     VIGIKDATGNLVRGSELIAKVGDRIAVLSGDDGTAFPLYACGGRGVISVVSNVAPREM
                     SEMWDAAKAGDWDRARKIHHKLRVLNNMLFAEPSPAPTKAAMSLLGRCTLDVRLPLVN
                     ATPKLIEDLRTEMKTQGLL"
     gene            14247..15848
                     /locus_tag="VFV99_26225"
     CDS             14247..15848
                     /locus_tag="VFV99_26225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062909.1"
                     /translation="MSQVLSEGSVLGGRYAIEARLGDGGMGTVYRARHQKFGRAFAIK
                     VLHAGLMQNPKVLKRFEREAEMAGRLRHTNVASVVDVGVTDDGVHYIAMEYASGVSLT
                     HLLADGPMAESRMLDIVKQLCRGLQHAHEAGLIHRDFKPDNVIVEPVAGGGEVARIVD
                     WGVSILREQAGDPTSDERLTTKGIVVGTPHYMAPEQARGAAMDHRVDLFALGLICYEL
                     LTGLLPFDGSGVDIARANMQTPTPPIAERAPGVRVDPVLEAIVRTLLAKNREDRPASG
                     SAALELFELYERDRASCALLLGVEIPVEDRRTGAIEELDDAEVADDAEDAPVGRAHSP
                     SQNQNGTRDAESPHDPERDRETDELASDNQWRRLAAVAAIFAIALAIVLYLGLRTTPR
                     ATALTIATADAREEVAVLETQRALPVADVPIVDVPGPAPTDEIALIPTKPPVKKAPPS
                     TGSGSGNAKPAVEPPSAAAVAKLYGAVGRELSALEAKKGMDATIELWPRYRWIRINEW
                     ITTPERRAHIATQLERLRTDIKAAG"
     gene            complement(15903..16670)
                     /locus_tag="VFV99_26230"
     CDS             complement(15903..16670)
                     /locus_tag="VFV99_26230"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5062910.1"
                     /translation="MGLVRESDAPVRREWFSARLFTVVIGAVAVIWVATVVVGGWKAS
                     HRRVVADRTIVAVAVAHRQITPDTIEWGLKLTTHGTDRMAALRELRAASEATRAYLTS
                     HEIKAAEMTIYPAETEEDIQTITHHRADGSEDSEDVPHGFNATQRIAMHSGDIARFLR
                     AFRAATLAAELGTAEIEEPTCTLSRLDDIQNELAVAVRRAVRAKAEADVAAMGGARLG
                     KLLNSDGESFNVGSTSLPACEQGTDASSTLHATYLLD"
     gene            complement(16730..17209)
                     /locus_tag="VFV99_26235"
     CDS             complement(16730..17209)
                     /locus_tag="VFV99_26235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5062911.1"
                     /translation="MMDSPSSEMSGGMRALTITQVGDLPPRPHAIVNVGDAMWKVVEA
                     MKTKGRGAVCVEEDGALVGIFTERDLVSRLDHDDALWSHVVVKDVMTPHPTVVRPYQS
                     VAEAMHLLVQGRRRHLPIVDDKGTIQGLLSIRDILGYVASKFPEEMMNLPPTPDHES"
     gene            complement(17206..17691)
                     /locus_tag="VFV99_26240"
     CDS             complement(17206..17691)
                     /locus_tag="VFV99_26240"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5062912.1"
                     /translation="MRRLQKTIEEIGWTKSVPRFAKTDMVGRAFDAMSRESHDCVLIV
                     DGDQLAGIFTSRDFLNRVAALRKDPQKLALGDVMTPSPRTLRPRDGVAFAINWMAIEG
                     FRNVPIVDDNSAILGVLTVWDVMRHLDAVFDEIEATPRPTPPKSDISEVINMVDLGGG
                     G"
     gene            complement(17724..19469)
                     /locus_tag="VFV99_26245"
     CDS             complement(17724..19469)
                     /locus_tag="VFV99_26245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062913.1"
                     /translation="MKLRGRFTLTLALAALVPISVAAVVTTQVIASRERGRYKADRDQ
                     AMQTLEREVSRLETGVRDTAVSLAAPDHPLVAEMLIDYAKGNGELDAAAQRKLRERSG
                     PFMHGLSLELLTITGPDDVVQVAPHYRAKIGDVDKSVRELAAAHAGSTFYAIESTMTG
                     GARAKENVLVAESAQLSRGEGYTVALAVGRRVSEDLLKSLRSDHVDARVVSADGKDEL
                     VAPGRRDWAVLAGHNMIPRTLYGPDGKAVAIVQVAFSNGDLDKILRDITLTSALLALG
                     ALGAVVLIGLFVARRTARDLDLLVEGSLAASRGDLDHRVPVRSKDEVGAVASAFNFMM
                     EDLKNSKEKLVIAERIAAWQDIARRLAHEIKNPLTPIQMAMDTLRKSWKKQHPSFDEI
                     MEESTRTVLEEADRLKKIVAEFSDFARMPKPELSRVDLNEIIRSALSLYQGAVKVESR
                     LASDLPEIDGDKGQLTQVLLNLVENGRDAIGNREDGRITVTTKRGEANDRATLIVEDN
                     GPGIPGDLKDKIFAPYFTTKQSKGGTGLGLAIVHRIVSDHGGRITLSDVPERGAKFTI
                     ELPLRSGTALLASRI"
     gene            complement(19466..20989)
                     /locus_tag="VFV99_26250"
     CDS             complement(19466..20989)
                     /locus_tag="VFV99_26250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5062914.1"
                     /translation="MKRPRVIDRRGSVRPGHAALLLVLAVALDAQGEGRPRYGGNVEA
                     TLLGAPASLDPLAAKSHAEATVVGLVFDTLYRVGANGVALPHLAAAQPVLDEKQTTVR
                     IAIRRGIRMHDGTELTAQDVAASLDRARQQARWLFAPVLAVKSDGDAVELSLRAPVPE
                     LTTLLAMPQLAITKAGRPPGDRPIGTGPYSVDSFDRAKKKIVLRAFDDHFAGRPYVDQ
                     LTLAWYDTPDGEARRFETGKAQISARGAAAFAGGQPTFRTDDVEGPPALLVFVGFGRA
                     HAEVTGDRAFRRALDLALSRGGLTTISSGERVVPTRSPVPVEAGGAALDPAGKAGDLP
                     AARAQLAVAAKRVAALASSQLGKLRLEILVEDTRPDDRQIAERVSLALDKLGIQAVIT
                     SLAASTLRERVAKGQCDLWIGQTAAPVTAQLVWWSAAFASGGDDWSVSALGGGAIDPG
                     VAGKEFATRLPIVPLMFRAVRMWHPTNLRGIAFDASGRPCYADVFYYGAATPTKGRP"
     gene            complement(20986..22017)
                     /locus_tag="VFV99_26255"
     CDS             complement(20986..22017)
                     /locus_tag="VFV99_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062915.1"
                     /translation="MKRLAAAALALAIAAPARVNAEAEAPRPPAGPDGWLHDLVRDVR
                     AKIDAAIVARPPKLVPPKKVDIHWKLAKLGSLDLGAPLIAMTGADLDGDGKGELYAVT
                     TRDVIALGVRNKRLEEIGRVAFAGEAAVPMPRDVVGSVSVETGAIVASVSSFAKSLHV
                     WWNDKTLVGSPGKPGFAMCPGEVVQLVPGRNYFGDATNQHYGARCRSGLVEPDGHPIR
                     VRAQLTLANHLEIAVERCAAANLGCQPARKHDYGAAGTAFDIADVDRDGNPELIYTGM
                     GAPGDPDLIKVVTLGDDEKRHAKLRKSFTAGGVAGVAVSDLDGNGVQDVAFALRIVGA
                     TRVDLWRIE"
     gene            complement(22014..24449)
                     /locus_tag="VFV99_26260"
     CDS             complement(22014..24449)
                     /locus_tag="VFV99_26260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5062916.1"
                     /translation="MPAKRSATKPSKPGKPGKQPKAPRKKWVTIVKWSAIAMLAVTAL
                     LAATVAFTFWMYGRDPKLPRIEKLADYKPKQVTTIVDKNDQRIGEIFTQRRTYVPYEK
                     IPPIVVDAFVAAEDNNYWEHEGVDYFGMVRAFFSNLRAGKTKQGASTITQQVVKNFLL
                     TPERSFKRKIQEIILARRLEKALSKQEIMALYLNEIYFGGRRYGVQEASRFYFGKDVS
                     QLNPGEAAVLASLPKEPETFYKALKQEKNPGRVKDRQIYVLNQLVKLGKLQQADAKKW
                     IDEPIKVVKDPFPFMKSAPEWVDLVKGDLKNQGIDTDTAGAVVRTTLDPSMQQAAQKA
                     LQAGLRAVDARHKVGRAKRNVGADKIELELAKLAKRLPSAGPKAKEVYEAIVMSVHDD
                     DHEIAVDLGKWPAAIALDTPEDERFNKDDKGNSLKPSERFKVGDVVDVMLASAVKKKV
                     DDDDDEDVVDVKVKHAQHRVAFAPGPQGAVVIMEVKTRKVRALVGGYSSKVGGFNRAI
                     DAHRQPGSSFKPYVYTTAIDSGRYTPATKVNDAPEVFDLWKPKNYESGKFEGPVLLRH
                     ALKKSINTVAIRVTFDMKPENIVTMAHKLGIVSELPKEMSLALGSGEVTPLEHVNAIA
                     TIAAGGIAMAPRFVEAINGKAIAQPQGTQVLRPEVAYVVTNMMQSVVKEGTATKANVL
                     KIPIAGKTGTSNEARDTWFVGFTPDYIVGVWIGYDDNRPMGGKETGGTTAVPVYVDVM
                     KSMQQPAKEFARPAHVVEKVIDRETGLLAPEGAPKSTTLSEVFVEGTEPTEVAPKPGE
                     VTEGSSVTGEYGD"
     gene            complement(24616..25257)
                     /locus_tag="VFV99_26265"
     CDS             complement(24616..25257)
                     /locus_tag="VFV99_26265"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5062917.1"
                     /translation="MAVCSALESLLTPDRIARIDAVIDARLASVAAAVEDTYDPHNAA
                     ATLRTCEALGLQELYVIEPGARFSAAKGVTRGADRWLDLRRFADAGAAIGDLKARNFR
                     VYATLPDAKISVEDVPVDSPLAIVFGNEHDGVSPAAIAACDGAITVPMFGFTESYNLS
                     VTVGLCMSRLAARRRAYIGALGDLDPERRARLRARWFALRIRGAVGVVDRKLA"
     gene            25326..>25811
                     /locus_tag="VFV99_26270"
     CDS             25326..>25811
                     /locus_tag="VFV99_26270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5062918.1"
                     /translation="MFVSEGVGRFVGDRYHIVRELGRGGMGVVYLGRDMRRDMDVAIK
                     FRGITHHDATLWLKREFRSVASLRHPNLVELYELVAHEQSCYFTMEYLPGVDPRNWVV
                     KPKYAPAELAALTDQPTRDLVLPEARTEMSLPPAGTADTISPAVPDVDFGRVRAVLAQ
                     LA"
BASE COUNT         4046 a         8871 c         8498 g         4396 t
ORIGIN      
        1 ggccctgcgc cgcatcgcgt ggctcggcgt cgtggtcctg atgttcgtgc tcgtgctggt
       61 agccggcggc aacgcgtcgg tgatcaaggc agcgacctcg tacaccgggc tcggcgcgcc
      121 gatcgcacgc ggcgtgcgca aggtcttcga tcgcgatcac gacggcttct cgcgcgtgct
      181 cggcggcggc gattgcaacg acagcgatcc gaagatccat cccggtgcgc ccgagattcc
      241 cgacaacggg atcgacgaga actgcgtcgg tggcgacgcg actgccaagc aaccgccaca
      301 cgacgtcggc ttcgccccgg tgcccgccgc cgtgccgcgc gacgcgaaca tcgtgctcat
      361 cacgatcgac acggcgcgcg ctgaccactt cggcatgtac ggctacaagc ggccaacgag
      421 cccgacgctc gacgcgatcg gcgcgcaggg caccgtgttc gaggccggct gggcgcacgc
      481 gccgtcgacg cgctactcga tgccggcgat cctgaccggg cggctgccgc tcgacgtcta
      541 ctacaactat gcgatcgaag gctggcccgg cctgtcgccg aaagcgacga cgctctgcga
      601 agcgctcgcg ccgctcggct ttgcgaccgg agcgatcacg aactacgagt acttcgatcg
      661 ctaccggggc atggaccaag gctgcgccga gtacgataac gagaacgcgc ggctgcacaa
      721 ccgagtgacg ggttcggggc cggagcagac gaagggaagc tcgtcgcgcg agcagaccga
      781 caaggcgatc gcgttcgtcg atcgccacgc gaaccagaaa tggttcttgt gggttcacta
      841 ttacgatttg cattacagct acgagccgca cccagggact tcgttcgggt tcgatcgcga
      901 agcactctac gacggcgaga ttcgcttcac ggattcgcac atcgcgcggc tcgtcgaggc
      961 actgcgccag cgcggccttt acgacaagac gatcatcgtg gtcaccggcg atcacggtga
     1021 aggcttcggc gaacacaacg tgctgatgca cggttatcac ctgtactcgc cgcagacgaa
     1081 ggtgccgttc atgatccgcg tccccggctt gcccgcgcgc cgctcgacga cgccggccgg
     1141 tcacgtcgac atcatgccga cgctcgtgaa cctcgccggc ggccaaccga accccgacat
     1201 ggagggtacg tcgctcgtgc ccgcgctcgc cggcgccgat cagccgcgca cgctgttcca
     1261 gcagctgtcg tacgagaaca atcacgagat gcgcggtggc gtcgacgcgc actgccacgt
     1321 catctacaac gtcagcccgg agacgagctg ggaggtttat cgcgtcgacc gcgatccgct
     1381 cgagaccgag gatgtctccg gtgacagcga ccaatgtgcc gacacgcgcc gcgcgttcga
     1441 gcgctggtac gacgcgggac aagtgccacc cggcgccgcg gaggcgctgc tgcccacgcg
     1501 ccccgcaatg gccaagccac tcgatgccga tctcggcgac agcgtgcgcc tgctcggcgt
     1561 cgacgtgccc gcccaagcga agcccggcga cgcggtcaac gtgacgtgga cgttcgaggc
     1621 acgcggcaag gtccctgccg actggaagct ctttgtccat gtcaaagggc cggggaacat
     1681 tttcatcaat ggcgatcaca agccgacgcg gccgttcgag tggtggaccg ctggccagtt
     1741 cattcagtat tcgacgacaa tcacactgcc ccgcttcgca cagccgggcg cgtacaccat
     1801 atgggtcggc atgttccgcg gtaaccagcg tgcaaccgtt cgcgcgccac acgcccgtgt
     1861 cgacaacaac gcggtcgaag cggccacgtt ccaggtggcg ccccaggtgg tgccgtgacc
     1921 ccgggcgtgc gccgctcgct cgagatcacg ggctgggcgc tgctcgccct gcccgcgctc
     1981 tatcagatcc tgctcttgtg ctccgcgatc ggcgggcgcg tcgcgtatcc gtatgacctc
     2041 gagtggatgg agggcggcat gctccaccac gcgctccgct tccagaccgg tcacggcatc
     2101 tactcggagc cgtccgtcga gttcatcccg tatctctaca cgccgctcta cccggccctg
     2161 ctcgcgatcc tcggcgagat cttcgggctc acgtacacgc tcggtcgcgt gatctcggtg
     2221 ctgtcgctgg tcggcatcgc gatcgtcggc gcaatctcga tctgctcgcc gcgcaaccgc
     2281 aacgctgcga tgttgccggc cgtcgtcggc acggtgctcg cgctcggttt gttcgccgcc
     2341 gtctatccat tcgtcgaggg ctggtacgac ctcgtgcgcg ccgacgcgct gttcttgttc
     2401 ctcgtcaccg ccggcctcgt cgccgcgatg tactggacgg atcaaggcga gggctggcgc
     2461 ggtcacggac gcgtcgccgc ggtcggcgcg atcctcgcgt tcgcattctt cacgaaacag
     2521 accggcatct tctacgtgct gctcggtggc gcgatcgtgt tgatcctgcg ttggcgccgg
     2581 ctcgccgcgt acatcgctgc ggcgggcgtc gtcggcctcg gcggctccgg cctgatgaac
     2641 ctgtcgagtg gcggctggtt ctggacgtac atccgcaaga ttcactccgc acacgacttc
     2701 aactacgacc gcttctgggt gtcgttcggc aacatcctgt ggcacttccg cgcgctcacg
     2761 atcgtgatca tcgtgacgct gatcgtcgtg gggtacacgt ggctgcgtcg gccggccgaa
     2821 ggctcgcgcc gcgagctacc gcggcagacg cggccgttct tgctctggac gtccgcattc
     2881 gtcgtatcga cgatcgtcgg cgccatcggc tggggcaccg agttcgcgca cttcaacgcg
     2941 tacatgccgg cgttcctgca cggcgcgctc gccgctggtg cagccgtgcc cgcgctcgcg
     3001 gcgtgcgccg gtatcctgtg gggcgcgcgc cagcactcgc aactcgtcgt ccacggtgca
     3061 gcgggtctcg ccgcgctgtt gctcgcggtc acgtgcctgc actggacgtg gcaaccgaaa
     3121 cgcttcaagc caacggacgc cgatgtcgcc gcgggcgatc gcctgatcaa gcggatcaag
     3181 gcgctcgacg gcgaggtgtg gatgccgtcg cacccctggt acctcgtgct cgccggcaag
     3241 cagccgcacg tccaccgcat gggcgtcaaa gacgtgacca cgcggcagaa ccgtcgcatc
     3301 gtcggcctcg acgacgccct cgagaaacat cgcttcagct cgatcgtgtt cgacaaccgc
     3361 gatctgttcc tcgagctacc gcggatccgc cagttctacc ggccggcgct gaagctgccc
     3421 gccgacgagc gaccgcggct gttcaccggc gcgatgatcg tgccggattc gatctgggtg
     3481 ccggcgctcg aggtcaagcc accggccggc gcacgcgtcg tgttcgactt cgagatgacg
     3541 tcgtggtcgg gctggacgaa agcaggctcg gcgtggggcc caggtccggt caccgagtcg
     3601 ctgccgggcc aggatctcgt gatcggtgca accggccagc gcttcgccga cagcatgcat
     3661 ggcggtgacg tcgcgaccgg acgactcacc tcgccgacgt tcccactcga cggcaatcgg
     3721 ctcacgctgc aggtcggtgg tggcaccgac gcgacgaagc tgcggatcga gctctgggtc
     3781 gagggtcaga tcgtcgcgtc gagtggggcg ccatcgctcg gtggtgacgt actgcgcgag
     3841 gtgagctggg acatcagtgc gcaacgcggg aagttcgcga cgctcgtgct cgtcgacgac
     3901 gcgacgaccg ccgacggtca catcgtcatc gacgacgtgt ggatctggga tcgctagcga
     3961 taccgcgtcg cgcgtagcgc gctagcgcga caatcccggc gagcgccgcc tggttcttgc
     4021 atcgctgccg aggatgctga tgcggcggcc gatctcgtat cgcgctggta tcgcgcttgg
     4081 cgcgatgtgc acgctcatgt tcgttgcggt cgcggccgtg gccgcggcgc tgttcggcat
     4141 cccaaacccg gcgtaccggc tgttcgagct cgtatcgggt gcgctgcccg gcgtggtcgt
     4201 cacgtcggcg atcgacatcg tcgtcaagac ggtgcacagc gcgggcgcga cctcgacagc
     4261 agcggccgcg aaggagatcg agcgcgcgat ggcgatcgcg ctcgcgacgg gtctcggcgg
     4321 cgtgctcggc gccgtcgtgg tgtggttcgt tcgccgtggc aagcacgcgc tgctgaccag
     4381 ccttgttgtc ggcacggtcg cgggctgcgt gtacgtcgtg atcgccggca tcagcgcgat
     4441 catgatcgtg gtctcgctcg ctggcttcgt cgcgtggggc ggcgtgctcg gcgtctcgat
     4501 ccgcgcgatg gcggagcacg cgggcgtgag cgcggagcgt cgccaggttc tcgtcgtgat
     4561 cggcgtcgcg accgccgcga cgtgtgccgt cgcgttcggc ctcgtcaggc tgctcggccg
     4621 tggcggcgct cggatcagcc gcatgctgcg ggcggaagac gaagccgcgc tgacctcggg
     4681 ccccgcggcg tcgccgtcgc ccgagtcgct gaccgcgcgc ttttcgcccg tcgacggcac
     4741 ccgcgcggag atcaccgaca acgcgcactt ctatcgcatc gacatcaacc tcaagccgcc
     4801 gtcgatcgat ggcgcgacgt ggacgctgcc gatcgaaggt ctcgtcggca agccgcgcgt
     4861 gctcacgctc gacgagttac gcaggcggcc ggcggtgagc cagatcatca cgctcgagtg
     4921 catctcgaat ccggtcggcg gtgatctgat cggcacgagc cggtggaccg gcatacggct
     4981 cgccgacctg ctcgcagagc tcgaggtcgc gtcgacggcg cgcgcgctgt cgttcgaggc
     5041 agcggatggc tttcacgaga cgtgcagcct cgacgacgct cgcgatccgc gcgcgctcct
     5101 cgtgtacgcg atgaatggcg agccgctgac cgaggtgcac ggctatccgc tccgcctcta
     5161 cctgccagac cgccacggca tgaagctacc gaagtggatc acccgcatgg agctcgtcgc
     5221 cgacccgcgg ccgggctact gggtcgagcg cgggtggagc gcggccgcga ttccgcacac
     5281 gacatccgtg atcgatgcgg tgacggcgac cggcgacggg gccgagcgca tgatgtcgat
     5341 cggcggcatc gcatacgccg gtgcgcgcgg catccagcgc gtcgaggtgc agatcgacga
     5401 cggtgcgtgg caggacgcgc agctacggac gcccgcactg agcccgctga cgtgggtgca
     5461 gtggcggctc gacgtgccgt atcgcaaggg caagcacacg attcgtgtcc gcgcgaccga
     5521 cggcaccggc gcgttgcaag acaccgtcgt gcgtccgccg catcccgacg gtgcgaccgg
     5581 catctacacg aagcgcgtcg acgtctagtg gcggatgatc acgtcgtagg cgtcgccgcc
     5641 gacgcggtag tacttccact cgcgatcgaa cgtccacgcg ccgaacaggt tgatcacgac
     5701 gcccgcgatc atcaggccct tcgcgagtcg ggttagcggc cgcgcgccga tcgcgagcag
     5761 catgatcaag aacaccatgt agtcgaggct gaaccggtag ccgaactgga cccagccgct
     5821 gttctgatag agcagcgacg gcagcgcgac acacgcgacg gtgatccaca gcgcgcggtg
     5881 aagcgccggc ttctcgcgcg gccacagcag gtagagcaag accggcgtcg tgaaccacag
     5941 cgcgagcccg tggccgccga tctgaatcca cggcgcgcgg ccgggaagct cgggaagcag
     6001 cgtgaagccg accgtgaggt tgcgcgacag atagtggtag ctcatcaggc cccactgctc
     6061 gatctgcggc gcctggcgga cctcgaggta cgtgtggccg aactccgtcg gcgagccgaa
     6121 gcggacgtag ttgtagatca tgcccgcgat cgcaaaggcg gcgaccggaa tcgcgaactt
     6181 cacgagcgtg cgcacgaacg cgcggcgatc gccctgggcc atgcgccacg cttcgagcat
     6241 gaacagcggg aacatgaagg ccatcggcgt gcgggtgagc gcggctgcac cgagcgcgag
     6301 gcctgcgatg atcgggcgct tcgcctcgat cgacgcccac gcgtagagca gcgcgagcac
     6361 gacaccgacg acgtgcgcgg tgtaccagac cttgccctgg accgcggaga agaacatgac
     6421 gctgccgaac gcgagcgtgc cgacgagcca gagatcctcg cgcaccgtgc gttgcgagaa
     6481 gcccgcctcg gcgagccggc gcagcagcag cagcgtgagc ggcaggatca atgcggcgac
     6541 gagcagcgtc gggatcacgt cgttgccatc gcggcccgag atcagcgccg acggcatcat
     6601 caggagcgtc ggcagcggcg ggaacgacat gtaccaggtg gtcccgcgga cgccgcgcac
     6661 ctggttcgcc ggcacctgct cgccgtcgag cgtgcggaac accggcttcg agatcatgcg
     6721 tttgccctcg acgatgcggc cgtcggcgag ctcgaccgtc tcgagcttgg cccagtcgtt
     6781 atcgacgagc ggcggatcga cgtgcgcctg cccgtgcagc cacgcatccg cctggaacac
     6841 gaactgcggc gcctgcgatt gcttcgcaat acggctgccc gcgagcgcgc cgtagacgac
     6901 gagcccgatc gcgaacagcg ctgcatacag ccgcacgggc tgacattacg ttgccgccgg
     6961 ccccgtgtca tagatccggg cagaccgccc actgggccgg tacgcgcggc gtggtagttt
     7021 gtcggggttg tcgacgcgac cgtcgccgat cgccgcggtc cccgcgctgc tgctggtcgt
     7081 ggttgcgttg tgggagacct gcgcgacccc gcgcgacgct gcggaggtcc cgggcgacaa
     7141 ggcgtgggcc aaggcggccg acgtcgtccg cgccggctac cagcccggcg acctgatcgt
     7201 gttcgcaccc gactggatcg atccggtggg ccgcctccac ctcggcgatt tgatcccggt
     7261 ggagatggcg gcgcgcatgg atgccgcgcg ctacggccgg atctgggagc tgtcgatccg
     7321 tggtgcacac gcggcagaca ccgcgggcct caccgcgacc tccgagcaga ccgtggctgg
     7381 catcaccgtg cggctgttca gccgcacgcc ggcgactgtg gtctcggacg tgcgtgacgc
     7441 actggcgagc gtgaaggtcg agggggccgc gcggccaacg ctcgagctga ccgaggtcgg
     7501 tttcgcgccg catcgctgca tccagatcgt cccgccgccg aaaggtacag tgcggatcac
     7561 gttcccgcag ctggcgctcg gcagcgagct cgtcggctac gccggcctcg ccgacatctt
     7621 cacccgccgc tcgccgcgcg gtccgggcaa gctcgccgtc gagatcggcg ggaaggtcgt
     7681 cgcgcgcatc gcgcctggcg tcgacgacgg ctgggtgcgg ttcgccgcgc cgacgacgcc
     7741 cggccccgcc gacgtcacgt tcatcgcgag cgcagacgat ccgaatcgat tgatctgctt
     7801 tgccgcggag gcgcggcgat gaagatcaac tggaagtggc ggccgagcaa gcagcgcatc
     7861 gtcggctggg tgctcgcgct gttcacggtg ctgctcgtca ccacgaacca gcaggacatc
     7921 ggcatcgcgc gcgacgaaac ggtctacttc ggcgccggca cgcgctacgc cgagtggtgg
     7981 ctcggcctcg tcacgttcaa gcacggcatc gcgaagaaat cgatcaccga cacgttcggt
     8041 ggcgcgccgg gcggcggcac gaactccgag catccgccgc tgatcaagac cgcgatgggg
     8101 ctgtcgcact ggctgctcta cgacaagctg cacgtcaccg acgagctcac ggcgtaccgc
     8161 ttccccggtg cgctgttcgc cgggttgctc gtgctgctcg tgtattcgat gacgctcgcg
     8221 ctgtggggct ttccggaagc cgtgatcgcc gcgctgtgca cgctgctgtt gccgcgcgcg
     8281 ctgttccacg ccgggctcgc gtgcttcgac gcgccgatca tgacgctgtg gttcgcgacg
     8341 atctatgcgt actggcgctg cctcgacgag cgcaagtggc cgtggcaggt cggcgtggtg
     8401 tttggcctcg cgctcgcgac gaagcacaac gcggtgctgc tgccgttcgc gctcggtctg
     8461 cactacgtcg tgatcgcgtg gcgcgcgggt aagtggcgcg gcctcgtcta tcacaagccg
     8521 cgcgtcgtgc tgtcgctcgc gctgctcggc ccgctcacgc tgatcgtgct gtggccgtgg
     8581 ctgtggttcg acacgttcgc gcacatcaag cagtggctgt cgtttcacac gagccacgtg
     8641 cactacaact tcgagtacct cggcaccaac tggaacgcgc cgcggttccc gtggcatgtc
     8701 gcgctcgtca cgacgctgtt cacggtgccg gtcgcgacgc tcgccgccgc atgcgcggga
     8761 gcgggcgtgc tcgtcgtgcg ctggcgacgg cgcgagaacg cggatcacga gcgcgcgccc
     8821 gcactgttgc tcgcgctgtc cgcggcggca tcgatcggcc cgttcttcct cggctcgacg
     8881 ccgatcttcg gcgccgagaa gcactggatg ccagcgctac cgacgctgtg cattgcggcc
     8941 ggcgtcggcg ccgcatgggc cgcgcggcaa gccgccgagt acgtgcgcgc gttgcgtccg
     9001 gtcggcgaag tgctcgcgcg ccgcgtggcg ctcggcatcg tcggcggcgc gatcgtgctc
     9061 gcatgcgcga cggagaccgt cgccgcgcag ccgtatgcgc tgacctggta caacgcgctc
     9121 gccggcgaag caccgggagg cgcggacctc ggcatgaacc ggcagttctg gggcgttgct
     9181 gcgcgcggcg tgctgccgtt catcgcgaac caattcccga cgcgcgtgta ctcgcacgac
     9241 gcaagtcctg cgtggggttg gtaccaccgc ctcggcttgc ttcggggctt tcccgacgcc
     9301 gggcaagaag gcgtcggcgt cgagcgctcc aacctcgcac tcgtcatcca cgagaagcac
     9361 ttcaaccgac acgactacat gatctggaaa tcgtacggca cggtgcgccc ggtgttcgtg
     9421 ctgcgcagcg agggcgtgcc gatcgtgacc gtctacaaac gacctgtccc gcgtccgtga
     9481 tcgataatgg gggttatgag cgaccccgtc gatccgctga cgcccacgca agctgcgacc
     9541 gagagcggcg agccggcgac gctcggtacc gcgccggtcg aacgcgtttc ggtgcgcgac
     9601 attccgacct acgagctcgg cgaagtgatc ggccgtggcg gcatgggcga ggtgatcgcc
     9661 gcgcgcgacc gcacgttcgg ccgcgaggtc gcgctcaagc gcatgcgtgc cgacagcccg
     9721 tcgcccgagc acgtcgagcg atttctgcgc gaagcacgga tccaggcgcg cctcgatcac
     9781 ccggcgatcg cgccggtcca cgagctcggc tacgacagcg acggcctgcc ctacttcacg
     9841 atgaagcgcg tcggcggcgc aacgctcgcg gccttgctcg aagccaagac cgagaaaccg
     9901 cagcgcctgt tgcgcgcgct cgtcgatgtt tgccatgcag ccgagctcgc gcactcgcgc
     9961 cgcatcgtcc atcgcgacct caagccatcg aacatcatgc tcggcgacta cggcgaggtg
    10021 tacgtcctcg actggggcgt cgcgcgcgtg atgaccggcg accgcgcgca gacgtcaccc
    10081 agcaccgacg acgtgccgtc gctcgacggc gtcacgaagg ccggtgcact gctcggcacg
    10141 cctggctata tggcgcccga gcaagttcgc ggcgaggccg tcggcccggc ggcggacgtc
    10201 tattcgctcg gctcgatgct gttcgagatt cttgccggcg agccgctcca tccgcgcaac
    10261 gacgcgatcg cgagcacgat gacgaacgtc agccgctcgc cgtcgcagcg caatccagcg
    10321 cgcgcggtgg cgcccgagct cgacacgatc tgtgccgccg cgctcgcgac cgatccgaat
    10381 ctgcgaccga gcgcaggtgc gatcgcgacc gcgatccagg actatctcga cggcgatcgt
    10441 gacgtcgagc gccgtcgcga gctggccggc aagctcgcga gctcggcacg cgccgcgctc
    10501 gagaagggcg actctgcaca cgcgatgcgc agcgccggcc gcgcgctcgc gttcgatccg
    10561 acctcgagtg aagcgaccgc gatcgtcacg cagctcgtgc tcgcgggccc gagcacgacc
    10621 gagacgcccg acgaagtaga cgccgcgctc gagaaagagg agcagcggct gatgcgccag
    10681 cgcagcaagc gcgcggtgtt gccgtacggc gcgatcttct cgaccctgcc gctcgtgccg
    10741 ttcctccacg tccagagcta cccgatgctc gccgcggtgt acgtcgcctt cgcgctgatg
    10801 gtgggaatca cgtggctgaa ctcgcgggtc ggcgtaccga tcctgctgac gctcaccggt
    10861 cacgcgatca cggcgctgtt gttctcgcga ctggccggcc cgttcatcat cacgccgatc
    10921 atgctgtgcg cgatcttgct gtcggcgacc tcgattccgt ggctcaaccg acgaccactc
    10981 gcgatcttgg tatggacatg cggcaccgtg atgctgccgt ttgtcctcga gtgggcgggc
    11041 gtgctcgcgc cgagctgggc aatgacgtcg gacggcatcc tctcgacggg caacgtgttc
    11101 gtgcagtcgc agatgccggc gtcgctcggc atcgcgggca atgtcgccgc aatctgcttg
    11161 gtcgcgttct acgcgcgcgg cattggccgc gaccggcgcg aggcacagcg ccggctgttc
    11221 ctccaggcgt ggcatctacg ccatttgctg ccgaagccgg ccgaatagcg gcatcagcga
    11281 tgcacgtcca cggcgtatgc ccagactgaa ttcgctgacc ggcttcgtgt tcattttgat
    11341 cctgctggcc ggtttcgggc ttgcctacgc gtcatacggc acgcttgcga acccgcggag
    11401 caccgtcatc atcctgctcg cgctgatcgt cgcaggcttc gtggcacgtg cgatctgcat
    11461 cgctgcggcg tggcagcgcg tcgtggtgct acgcctcgga aagtttcgcg cactgcgcgg
    11521 cccggggctg ttcggcatca ttccgatcat cgactcgatc ccctacacga tcgacatccg
    11581 cgtcatcacg tcgtcgttca aggccgagaa cacgctgaca cgtgacacgg tcccggtgaa
    11641 cgtcgacgcg gtgttgttct ggaaggtgat cgacccgcag cgggccgcgc tcgcggtcgc
    11701 cgactacgag agcgcgattc gctgggcggc acagacggcg ctgcgcgacg tgatcggcaa
    11761 gacgatgctc gccgacatgc tcgagggccg cggcaagatc tcgctcgagc tgcaggagat
    11821 gatcgactcg cgcaccgaac cgtggggtgt caacgtgatc gccgtcgagc tgcgcgacgt
    11881 gctcatcccg tcggggctgc aggatgcgat gtcgatgcaa gcgcaggccg agcgcgagcg
    11941 acaggcacgc gtcattctcg gcgactccga gcgccagatc gcggagaagt tccaggaagc
    12001 cgcgcgcagc tacgcgaacg atccgaccgc gctgcatctg cgcgcgatga acatgctgta
    12061 cgagggcctc aagcagaacg cgacgatcgt gctggtgccg agcaccgccg tcgagacgat
    12121 gcaactgggt aacgtggctg gcatcaccgc gctcgccgag ctgggcaagc agccgacgag
    12181 ccactaacgg gctacggccg gaacggctgc tcgtctttac cgcggactgg attcgcgagc
    12241 cggcgcgcaa ccgcgtcctg ctcgtctttc gtcaaccggc gcacgtagac gccagcggcg
    12301 tcgatgctgc gcgcgccgaa gaagccctcc gacaccgcgt actcgacctc gatgatcatg
    12361 taggggtcat cgccgatcca catcagctcg tgcttgcggg gaatcccgac ccacgagctg
    12421 agcgtgaact tgtcgcgcga tccgtcctcg ttgaggacga ccatcgtgat gcgctcggta
    12481 gcggtcattc gcccgcgaac cctagcacgt cgagcatgtc gtaacgaccc ggagctttgc
    12541 cgacgaccca cgcggccgcc gtgagcgcgc ccgcggcgaa aatcgtccgc gacgtcgcac
    12601 gatgcgcgat ttccacgcgc tccgcggcgc cgaagaacgt cgcgacgtgc tcgccgatga
    12661 tgtcgccgcc gcgcaccgtc gacacgccga tctcgccgcg cggccgcggc ccgacgtcgc
    12721 cgtcgcgggt gtggcgcttg accttgtcgt agtcgacgcc gtgacccgcg gcgatcgcac
    12781 gtgcaatcgc gagtgcggtg ccagacggcg cgtcgcgctt catcttgtga tgcgtctcga
    12841 cgatctcggc gtcccagtcg ggcccgagca ccttcgccgc ttgcttgacg aggccgagca
    12901 cgaggttgac gccaagcgag aagttcgccg cgacgaccac cggcgcgacc ttcgcgagct
    12961 ctgcgatcgc acgttcctcg gactcgccga ggccggtcgt gccgatcacc gcggccgtct
    13021 tcttcgcgag tgctgccttt gccgcagcac gtgtgcccgt cggcgacgtg aagtcgacgt
    13081 acacctgcgc cttcgcgagg ccggccgcca catcctcacc gagatcgacc gtcgagacca
    13141 cgcgcacatc ctcgcgcgcc tttgcggcct cgatcaccga gcggcccatc ttgcccgatg
    13201 gaccgagcac gcagacatcg atcacagcag gccctgcgtc ttcatctcgg tgcgtaggtc
    13261 ttcgatcagc ttcggcgtcg cgttgacgag cggcagccgc acgtcgagcg tgcaacgacc
    13321 gagcagcgac atcgccgcct tcgtcggtgc ggggctcggc tccgcgaaca gcatgttgtt
    13381 gaggacgcgc agcttgtggt ggatcttgcg cgcgcgatcc caatcgccgg ccttcgccgc
    13441 gtcccacatc tcgctcatct cgcgcggcgc gacgttcgac acgaccgaga tcacgccgcg
    13501 gccgccgcac gcgtagagcg ggaacgctgt gccgtcgtcg cccgacagca cggcgatccg
    13561 atcgccgacc ttcgcgatca gctcgctgcc gcgcacgagg ttgccggtcg catccttgat
    13621 accgatgacg ttgtcgaagt cagcgagacg catgaccgtc tcgcactgca tgtcgcacgc
    13681 ggtccgcgac ggcacgttgt agagaatgat cggcagcttc gtcgccttcg cgatcgcttc
    13741 gaagtggcgg aacaggcctt cctggttcgg ccggttgtag tacggcgtga cgtgcagcag
    13801 cgcatcggcg ccggcctcct ccgaggcttt cgtcagcgtg agcgcttcgc tcgtcgcgtt
    13861 gccgccggcg ccagcgatca ccggcacgcg gcccttcgcc gctttgacag tcgccgtgat
    13921 caccgcgacg tgctcgtcga cgtcgagcgt cgccgactcg ccggtggtgc cgaccgacac
    13981 gatgccgtcg acgccgttct cgatctgcca gtcgacgagt ttggcgaggc cctcgaagtc
    14041 gactttgccg ttcttgaacg gcgtcacgag cgcagtgaga caaccctcga tcgtcggaag
    14101 cttcgccata ggagacctca tacatctaac gccaagccac gggaagcaat tccgcgggca
    14161 tagacgcctc ggtcattccc gcatggaaag ggctgcgtta gggcgattca ctcgcaaatc
    14221 gcaaagatgc gatgctgctc ggtggggtgt cacaggttct ttctgaaggc agcgtcctcg
    14281 gcggccggta cgcgatcgaa gcccggctcg gcgacggcgg catgggcacg gtctatcgcg
    14341 cgcgccacca gaagttcggc cgcgcgttcg cgatcaaggt cctgcacgcg ggcctcatgc
    14401 agaaccccaa ggtgctgaag cgcttcgagc gcgaagccga gatggcgggt cggctccgcc
    14461 acacgaacgt ggcgtcggtc gttgacgtcg gtgtcaccga cgacggcgtg cactacatcg
    14521 cgatggaata cgcgtcgggc gtctcgctca cacacctgct cgccgacggt ccaatggccg
    14581 agtcgcgcat gctcgacatc gtcaagcagc tctgtcgcgg cctgcagcac gcgcacgagg
    14641 ccggcctcat ccatcgcgac ttcaagcccg acaacgtgat cgtcgagccc gtcgccggtg
    14701 gcggcgaggt cgcgcgcatt gtcgactggg gcgtctcgat cctgcgcgag caggcgggtg
    14761 atccgacgag cgacgagcgg ctcaccacga aaggcatcgt cgtcggcacg ccgcactaca
    14821 tggcgcccga gcaagcgcgc ggcgccgcga tggaccatcg cgtcgatctc ttcgcgctcg
    14881 gcctcatctg ctacgagctg ctcaccggcc tgttgccgtt cgacgggtcg ggcgtcgaca
    14941 tcgcgcgcgc gaacatgcag acgccgacgc caccgatcgc ggagcgtgcg cccggcgtgc
    15001 gcgtcgatcc cgtgctcgaa gcgatcgtgc gcacgctgct cgcgaagaac cgcgaggacc
    15061 gcccggcgag tggcagcgct gcgctcgagc tgttcgagct gtacgagcgc gaccgcgcaa
    15121 gttgtgcgct gctgctcggc gtcgagatcc cggtcgagga ccggcgcacg ggtgcgatcg
    15181 aagagctcga tgacgccgag gtcgcagacg atgccgagga cgcccccgtt gggcgcgccc
    15241 acagcccgag ccaaaaccag aacggcacgc gtgacgcgga gtctccacac gatcccgagc
    15301 gcgatcgcga gaccgacgag ctcgcatccg acaatcagtg gcgccgtctc gccgcggtcg
    15361 ctgcgatctt cgcaatcgcg ctcgccatcg tgctctacct cggtctgcgc acgaccccgc
    15421 gtgccacggc cctcacgatc gcgacagccg acgcgcgcga ggaggtcgcc gttctcgaaa
    15481 cccagcgcgc actgcccgtc gccgacgtgc cgatcgtcga cgtgcccggc ccagcgccca
    15541 ccgacgagat cgcgctcatc cccacgaagc cgccggtcaa gaaggcacct ccgagcaccg
    15601 gaagcggcag tggcaatgcg aagccggcgg tcgagccccc gagcgcggcc gcggtcgcca
    15661 agctctacgg cgccgtcggc cgcgagctct ctgcactcga ggcgaagaag ggcatggacg
    15721 cgacgatcga gctatggccg cgctaccggt ggatccgcat caacgagtgg atcacgacgc
    15781 cggagcgccg cgcgcacatc gcgacgcagc tcgagcgcct gcgcacagac atcaaggcag
    15841 cgggataaag gggtctgacc ggtctgaccc cttttctgca gctcgtcggc gagccggccg
    15901 cctcagtcga gcaggtaggt cgcgtgcaac gtcgagctcg cgtccgtgcc ctgttcgcac
    15961 gccggcagcg acgtgctccc gacgttgaag ctctccccgt cggaattgag gagcttgccg
    16021 agccgtgcgc cccccatcgc cgcgacatcc gcttcggcct ttgcgcgtac cgcgcgacgt
    16081 acagccacgg cgagctcgtt ctggatgtcg tcgagtcggc tcagcgtaca cgtcggttcc
    16141 tcgatctccg ccgtgccgag ctcggctgcc agcgttgccg cgcggaatgc gcggaggaag
    16201 cgcgcgatgt ctcccgagtg catcgcgatc ctctgcgtcg cgttgaatcc gtgcggcacg
    16261 tcttcgctat cttcgctgcc atcggcacgg tgatgcgtga tcgtctggat gtcctcttcg
    16321 gtctcggccg gatagatcgt catctcggct gccttgatct cgtggctcgt cagatacgca
    16381 cgggtcgctt ccgacgccgc gcgcagctcg cgcagggcgg ccatgcgatc cgtcccgtgc
    16441 gtggtgagct tgagccccca ctcgatggtg tcgggcgtga tctgccggtg cgcgaccgcc
    16501 actgccacga tggtgcggtc agccaccacg cgccggtgcg acgctttcca cccaccgacg
    16561 accactgtcg cgacccagat gaccgcgacc gctccgatga cgaccgtgaa taagcgcgca
    16621 ctgaaccatt cgcgacgcac cggagcgtcg ctctctctga ctagacccat gggctcctcc
    16681 tgattcggtg agacaccgag ccacggagcc ggttgctagc aatcgcgcgc tacgactcgt
    16741 ggtcgggcgt cggcggaaga ttcatcatct cctcggggaa cttgctcgct acgtagccga
    16801 ggatgtcgcg aatcgacagc aggccctgga tggtgccctt gtcgtcgacg atcggtaggt
    16861 ggcggcggcg gccctgcacg agcaggtgca tcgcttcggc gaccgactga tacgggcgca
    16921 cgaccgtcgg atgcggagtc atgacgtcct tgacgacaac gtgtgaccac agtgcatcgt
    16981 cgtgatcgag gcggctgacg aggtcgcgct cggtgaagat gccgacgagc gcgccgtctt
    17041 cttcgacgca caccgcgccg cggcccttcg tcttcatcgc ttcgacgact ttccacatcg
    17101 cgtcgccgac gttgacgatc gcgtgcgggc gcggcggcag atcgccgacc tgcgtgatcg
    17161 tgagcgcgcg catgccaccg ctcatctcgg agctcggtga atccatcatc cgccacctcc
    17221 gaggtcgacc atgttgatga cttcggagat gtcgctcttc ggcggcgtcg gccgcggcgt
    17281 cgcttcgatc tcgtcgaaca cggcatcgag gtgacgcatc acgtcccaca ccgtgagcac
    17341 gccgaggatc gcgctgttgt catcgacgat cggcacgttg cggaagcctt cgatcgccat
    17401 ccagttgatc gcgaacgcga cgccatcgcg cggccgcagc gtgcgcgggc tcggcgtcat
    17461 cacgtcgccg agtgcgagct tctgcggatc cttgcggagc gcggcgacgc gattgaggaa
    17521 gtcgcggctc gtgaagatgc cggcgagctg gtcgccgtcg acgatgagga cgcagtcatg
    17581 cgactcgcga ctcatcgcgt cgaacgcgcg gccgaccatg tcggttttcg cgaaccgcgg
    17641 gacgctcttc gtccagccta tctcctcgat ggtcttttgg agtcgacgca tacaccctcc
    17701 ctcccctcca ccataaccgc atttcagatg cgcgaggcga gcaaagccgt accgctgcgc
    17761 aggggcagct cgatggtgaa tttcgcacca cgttcaggaa cgtcggaaag cgtaatcctg
    17821 cccccgtgat cggagacgat gcggtgcacg atcgcgagac cgagtccggt cccacctttg
    17881 gactgcttcg tcgtgaagta gggcgcgaaa attttatctt ttaggtcgcc ggggatgcct
    17941 ggaccgttgt cctcgacgat cagcgtggcg cgatcatttg cctcaccgcg cttggttgtc
    18001 acagtgatgc ggccgtcctc tctgttgcct attgcatcac ggccattttc gacgaggttc
    18061 agcagaacct gggtcagctg gcccttatcc ccgtcgatct cggggagatc gctcgccagc
    18121 cgggactcga ccttgaccgc gccttggtac agcgagagcg ccgagcgaat gatctcgttg
    18181 aggtcgacgc gcgacagctc gggtttcggc atgcgcgcga agtcggagaa ctcggcgacg
    18241 atcttcttga ggcgatcggc ctcctcgagc acggtgcgcg tcgactcttc catgatctcg
    18301 tcgaacgacg ggtgctgctt cttccagctc ttgcgcaacg tgtccatcgc catctggatc
    18361 ggcgtgagcg gattcttgat ctcgtgcgcg agccggcgcg cgatgtcctg ccaggccgcg
    18421 atgcgctcgg cgatcacgag cttctctttg gagtttttga gatcctccat catgaagttg
    18481 aacgcggacg caacggcgcc gacctcgtcc ttgctgcgca ctggcacgcg gtgatcgaga
    18541 tcgccacgcg atgccgcgag tgaaccttcg acgagcagat cgagatcacg agccgtgcgg
    18601 cgcgcgacga acaggccgat aagcacgacg gcaccgagtg cgccgagtgc gagcagcgct
    18661 gacgtgagtg tgatgtcgcg taggatctta tcgaggtcgc cgttggaaaa tgcgacctgg
    18721 acgatggcga ccgccttgcc gtccggtccg tacagcgtcc gcggaatcat gttgtgaccg
    18781 gcgagtacgg cccaatcgcg acgaccaggc gcgacgagct cgtccttacc gtcggcgctg
    18841 acgacgcgcg catccacatg gtccgagcgg agcgacttca acaagtcctc gctcacccgg
    18901 cgcccgacag cgagtgcaac cgtgtagccc tcaccgcggg acagctgcgc ggactcggca
    18961 accagcacgt tctcctttgc gcgagcgccc ccggtcatcg tcgactcgat cgcgtaaaac
    19021 gtcgagcccg catgcgccgc ggcgagctcg cgcacgctct tgtcgacgtc gccaatcttt
    19081 gcgcgataat gcggcgcgac ctgcacgacg tcatcgggac ccgtgatcgt gagcagctcg
    19141 agcgacaagc cgtgcatgaa cggaccggag cgctcgcgca gcttgcgttg agcggccgcg
    19201 tcgagctcgc cgtttccctt cgcgtagtcg atcagcatct cggcgacgag cggatggtcg
    19261 ggcgcggcga gcgagactgc agtatcgcgt acgcccgtct ccaaccgcga gacttcgcgc
    19321 tcgagagtct gcatcgcttg gtcgcgatcg gccttgtagc gaccgcgctc gcgtgacgcg
    19381 atcacttgcg tcgtcaccac cgctgcgacc gagatcggca cgagcgcggc aagggcgagc
    19441 gtcaacgtga accggccgcg cagcttcatg gccggccttt tgtcggcgtc gccgcgccgt
    19501 agtagaagac gtccgcgtag cagggtcggc cgctcgcatc gaacgcgatg ccgcgcaagt
    19561 tcgtcgggtg ccacatccgc accgcgcgga acattagcgg cacgatcggc agccgcgtcg
    19621 cgaactcctt gccggctacg cccggatcga tcgctccacc accgagcgcg ctcaccgacc
    19681 agtcgtcgcc gccggacgcg aacgctgcgc tccaccacac gagctgcgcg gtcaccggtg
    19741 cggcggtctg gccgatccac agatcgcact gccctttcgc gacgcgttcg cgcagcgtgc
    19801 tcgccgcgag cgatgtgatc acggcctgga tgccgagctt gtcgagcgcg agcgacacgc
    19861 gctccgcgat ctggcggtca tctgggcggg tgtcctcgac gaggatctcg agccgcagct
    19921 tgccgagctg actgctcgcg agcgctgcta cacgcttcgc cgcgacggcg agctgggcgc
    19981 gcgcggccgg caagtcgccc gctttgcccg cgggatcgag cgcggcgcca cccgcttcga
    20041 ccggcaccgg cgagcgtgtc ggcaccacgc gttcgcccga gctgatcgtg gtgagcccac
    20101 cgcgcgacag tgcgagatcg agcgctcgac ggaacgcacg atcgccggtg acctccgcgt
    20161 gggcacgacc gaacccgacg aacacgagca gtgccggcgg accttcgacg tcgtcggtgc
    20221 gaaacgtcgg ctggccgcct gcgaatgccg ccgcaccgcg cgctgaaatc tgcgccttgc
    20281 cggtttcgaa gcgacgcgct tcgccgtcgg gcgtgtcgta ccaggcgagc gtgagctgat
    20341 cgacgtacgg ccggcccgcg aagtgatcgt cgaacgcgcg caacacgatc ttcttcttcg
    20401 cgcgatcgaa actatcgacg ctgtacggcc cggtgccaat cggacgatcg cccggcggcc
    20461 taccagcctt cgtgatcgcg agctgcggca tcgcgagcag cgtcgtcagc tcggggaccg
    20521 gtgcgcgcag cgagagctcg accgcgtccc cgtcgctctt caccgcgagc acgggtgcga
    20581 acagccagcg tgcttgctgg cgcgcgcgat cgagcgatgc cgcgacatct tgcgcggtga
    20641 gctcggtgcc atcgtgcatg cgaataccgc ggcgaatcgc gatgcgcacg gtcgtctgct
    20701 tctcgtcgag gaccggctgc gctgcggcga gatgcggcag ggcaacgcca tttgcaccga
    20761 cccgatacag cgtgtcgaac acgaggccga ccacggtcgc ctcggcatgg ctcttcgcgg
    20821 cgagcggatc gagtgacgcc ggtgcgccga gcagcgtcgc ctcgacatta ccgccgtagc
    20881 gcggacggcc ctcgccttgc gcgtcgagcg cgaccgcgag cacgagcagc agcgcggcgt
    20941 gccccggccg caccgagcca cgccgatcga tgacacgggg acgcttcact cgatcctcca
    21001 cagatcgacg cgtgtcgcac cgacaatgcg cagggcgaac gcgacatcct gcacgccgtt
    21061 gccgtcgaga tcgctcaccg cgacaccggc gacgccgccg gctgtgaacg atttgcgaag
    21121 ctttgcgtgg cgcttctcgt cgtcgccaag cgtcacgacc ttgatcagat ccggatcgcc
    21181 cggcgcgccc atgcccgtgt agatcagctc ggggttgccg tcgcgatcga cgtccgcgat
    21241 atcaaacgcg gtgccagctg ccccgtagtc gtgcttgcga gcgggctgac agccgaggtt
    21301 cgccgccgcg cagcgctcga ccgcgatttc gaggtggttc gcgagcgtga gctgcgcgcg
    21361 cacgcgaatc ggatgaccat ccggctcgac gagaccgctg cgacagcgcg cgccgtagtg
    21421 ttggttcgtc gcgtcgccga agtaattgcg gcccggcacg agctgcacga cttcgcccgg
    21481 gcacatcgcg aaacctggct tccccggcga tccgacgagc gtcttgtcgt tccaccacac
    21541 gtgcaggctc ttcgcgaagc tggagacact cgcgacgatc gcgccggtct cgaccgacac
    21601 actgccgacg acatcgcgcg gcatcggcac cgccgcttcg cccgcaaacg ccacgcgccc
    21661 gatctcttcg agccgcttgt tgcgcacgcc gagcgcgatc acgtcgcgcg tcgtcaccgc
    21721 atacagctcg cctttgccat caccgtcgag atcggcgccg gtcatcgcga tgagcggcgc
    21781 gccgagatcg agcgagccga gcttcgcgag cttccagtgg atgtcgacct tcttcggcgg
    21841 tacgagcttc ggcggccgcg cgacgatcgc agcgtcgatc ttcgcgcgca cgtcgcgcac
    21901 gagatcgtgg agccagccgt caggcccggc cggcggtcgc ggcgcttcag cctctgcgtt
    21961 cacgcgagcc ggcgcagcga tcgcaagagc cagtgcagcg gcggcgagcc gcttcaatcg
    22021 ccgtactctc ccgttaccga gctgccctcg gtcacctcgc ccggcttcgg cgcgacctcg
    22081 gtcggctccg tgccctcgac gaacacctcg ctgagcgtcg tgctcttcgg cgcgccttcg
    22141 ggcgcgagca gaccggtctc gcgatcgatc accttctcga cgacgtgcgc cggccgcgcg
    22201 aactccttcg ccggctgctg catcgacttc atgacgtcga cgtagaccgg caccgctgtc
    22261 gtaccgccgg tctccttgcc gcccatcggc cgattgtcgt cgtagccgat ccacacgccg
    22321 acgatgtagt cgggcgtgaa gccgacgaac cacgtgtcac gcgcctcgtt cgacgtaccg
    22381 gtcttacccg cgatcggaat cttgagcacg ttcgccttcg tggccgtgcc ttccttcacc
    22441 acggactgca tcatgttcgt cacgacgtac gcgacctcgg gacgcagcac ctgcgtgccc
    22501 tgcggctgag cgatcgcctt gccgttgatc gcctcgacga atcgcggcgc catcgcgatg
    22561 ccgcccgctg cgatcgtcgc gatcgcgttg acgtgctcga gcggcgtcac ctcgcccgag
    22621 ccaagcgcga gcgacatctc cttcggcagc tcgctcacga tgccgagctt gtgcgccatc
    22681 gtcacgatgt tctcgggctt catgtcgaac gtcacccgga tcgcgaccgt gttgatcgac
    22741 ttcttgagcg cgtgccgcag gagcaccggg ccttcgaact tgcccgactc gtagttcttc
    22801 ggcttccaga ggtcgaacac ctcgggcgcg tcgttcacct tcgtcgcggg cgtgtagcgg
    22861 ccgctgtcga tcgcggtcgt gtagacgtac ggcttgaagc tcgagcccgg ctggcgatgc
    22921 gcgtcgatcg cgcggttgaa gccaccgacc ttcgacgagt agccgccgac gagcgcgcgc
    22981 accttgcgcg tcttcacttc catgatcacg accgcgccct gcgggcccgg cgcgaacgcg
    23041 acgcggtgct gtgcgtgctt gaccttgacg tcgacgacgt cctcgtcgtc atcgtcatca
    23101 accttctttt tcacagcgct cgccagcatc acgtcgacga catcgccaac cttgaaccgc
    23161 tcgctcggct tgagcgagtt gcccttgtcg tccttgttga agcgctcgtc ttccggcgta
    23221 tcgagcgcga tcgcggccgg ccacttgccg aggtcgacgg cgatctcgtg gtcgtcgtcg
    23281 tgcacggaca tcacgatcgc ttcgtaaact tccttcgcct tcgggcccgc gctcggcagc
    23341 cgcttcgcga gcttcgcgag ctcgagctcg atcttgtcgg cgccgacgtt gcgcttcgca
    23401 cggccgacct tgtgccgcgc atcgacagcg cgcagaccgg cctgcagcgc cttctgcgcc
    23461 gcttgctgca tgctcggatc gagcgtcgtg cgaacgaccg cacctgcggt atcggtgtcg
    23521 atgccctggt tcttgagatc acccttcacg agatcgaccc actccggcgc gctcttcatg
    23581 aacgggaacg gatccttgac gaccttgatc ggctcgtcga tccacttctt cgcatccgcc
    23641 tgctgcagct tgccgagctt gacgagctgg ttgagcacgt agatctggcg atccttcacg
    23701 cgccccgggt tcttctcctg cttgagcgcc ttgtagaacg tctcgggttc cttcggcagg
    23761 ctggcgagca ccgcggcctc gcccggattg agctgcgaga catccttgcc gaagtagaac
    23821 cgcgacgctt cctgcacgcc gtagcgccgg ccgccgaaat agatctcgtt gagatagagc
    23881 gccatgatct cctgcttcga cagcgccttc tcgaggcgac gcgcgaggat gatctcctgg
    23941 atcttgcgct tgaatgaccg ctccggcgtg agcaggaagt tcttcacgac ctgctgcgtg
    24001 atcgtcgacg cgccttgctt ggtcttgccg gcacgcaggt tcgagaaaaa cgcgcgcacc
    24061 atgccgaagt aatcgacgcc ttcgtgctcc cagtagttgt tgtcctcggc ggcgacaaac
    24121 gcgtcgacga cgatcggcgg gatcttctcg tacggcacgt aggtgcggcg ctgcgtgaaa
    24181 atctcgccga tgcgctgatc gttcttgtcg acgatcgtcg tgacctgctt cggcttgtag
    24241 tcggcgagct tctcgatgcg cggcagcttc ggatcgcggc cgtacatcca gaacgtgaac
    24301 gcgacggtcg cggcgagcag cgcggtgacc gcgagcattg cgatcgccga ccatttgacg
    24361 atcgtgaccc acttcttgcg tggcgccttc ggctgcttgc ccggcttgcc gggcttgctc
    24421 ggtttggtgg ccgaccgctt cgcaggcacg gtcgcttgcg gggattgtga ggcccacatt
    24481 tttagcgtgg cacccgggtg cggcatgacg gccgaagagt gcaactcggg taccggtccc
    24541 aaggtggcat acgcatggat aatcccagca gtgcggctgg tttgccacac ggtgtgtgtc
    24601 gggtagccca catgatcatg cgagcttgcg atcaactacg ccgacggcgc cacgaattcg
    24661 cagggcgaac caccgtgctc tgagcctggc tcgacgctcg ggatcgagat cgcccagcgc
    24721 tcctatataa gcccgccggc gcgccgcgag ccggctcatg cacaaaccga ccgtgacaga
    24781 caggttgtag ctctcggtga aaccgaacat cggcaccgtg atcgcgccat cgcacgccgc
    24841 gatcgccgca ggtgagactc cgtcatgctc gttgccgaat acgatcgcca gcggcgagtc
    24901 gaccggcacg tcctcgaccg agatcttggc gtcgggcagc gtcgcgtaga cacgaaagtt
    24961 gcgtgccttc agatccccga tcgcggcacc cgcgtcggca aaccgacgga ggtcgagcca
    25021 gcggtccgcg ccccgggtga cacctttcgc ggcggagaat ctcgcaccgg gctcgatcac
    25081 gtacaactcc tggaggccga gcgcctcgca ggtccgcagc gtcgccgcgg cattatgcgg
    25141 gtcgtaggta tcctcgacgg cggcagcgac cgaggcgaga cgcgcatcaa taaccgcatc
    25201 gatccgagcg atgcggtcgg gcgtcagcag tgattcgagc gcgctgcaca cggccatggg
    25261 cccgtatcgg tcgatcaact tctcgatcgc atctttcact gagctgcatg ttaccgttcg
    25321 cccgaatgtt tgtgtccgag ggcgtcggtc gcttcgtcgg cgatcgctac cacatagttc
    25381 gcgagctggg gcggggaggc atgggcgtcg tctacctcgg tcgcgacatg cgccgcgaca
    25441 tggatgttgc gatcaagttc cgcggcatca cgcaccacga tgcgacgctg tggctcaagc
    25501 gcgagttccg ctcggtcgca tcgctccgcc acccgaacct ggtcgagctg tacgaattag
    25561 tcgcgcacga gcagtcctgc tacttcacga tggagtacct gcctggcgtc gacccgcgga
    25621 attgggtcgt gaagccgaag tacgcaccgg ccgagctcgc cgcgctcacc gaccagccga
    25681 cgcgcgatct cgtgctgccg gaagcgcgca ccgagatgtc gctgccaccc gcggggactg
    25741 ccgacacgat ctcgcccgcg gtccccgacg tcgacttcgg tcgcgtacgc gccgtgctcg
    25801 cgcagctcgc c
//
LOCUS       DASUFX010000646         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1215306, whole genome shotgun sequence.
ACCESSION   DASUFX010000646 DASUFX010000000
VERSION     DASUFX010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215306"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFV99_26275"
     CDS             <1..192
                     /locus_tag="VFV99_26275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062919.1"
                     /translation="EGISELHKAQDLEPLRSRAYVAEARWLASKGRTDEAIAKAREAT
                     IVEPTDQEASSLLNSLVGK"
     gene            189..890
                     /gene="nth"
                     /locus_tag="VFV99_26280"
     CDS             189..890
                     /gene="nth"
                     /locus_tag="VFV99_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5062920.1"
                     /translation="MTAVAATTDSLAPRDRRARATKIHSILDELFPGPAIPLDHGDPF
                     QLLVATILSAQCTDARVNMVTPALFKRAPDARTMAKMTAAQILPLIRTCGLAPGKAKS
                     LAAMSRALVADHGGDVPGDFDALENLAGVGHKTASVVMAQAFNVPAFPVDTHIHRLAA
                     RWQLSRARTVEETERDLKAVFPREAWNVLHLQIIYFGRTYCPARGHAPETCPICSWAM
                     SKARAAREARAQRRS"
     gene            966..1850
                     /locus_tag="VFV99_26285"
     CDS             966..1850
                     /locus_tag="VFV99_26285"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5062921.1"
                     /translation="MRRPLLVAVVVLSGCWKLQGIPTNHQLEATFPASVQTTDLPGQF
                     KVATFNIHMEPGHKVATALLADRALRDVDVIALEEVPRENGSTCSSACAMGHELGMYS
                     VFAPAHSKDGDDFGVAILSRVPITSARVIELPWNDVHFNAGRRIALVATVQHEGRPIT
                     VYAVHLENRLTASDRKRQMKPVLVDASQQRTPVIIAGDFNTNPFTWISHWIPLPTGAH
                     QQHALESFVRSYGFDTPVAGSGATHRYIGMKLDAIYTRGFDTKHFAVAHAKDVSDHMA
                     LWAIVAPTPQPPTVAAAK"
     gene            2009..2668
                     /locus_tag="VFV99_26290"
     CDS             2009..2668
                     /locus_tag="VFV99_26290"
                     /inference="COORDINATES: protein motif:HMM:NF027619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5062922.1"
                     /translation="MTTTAKTEAARRGAVDKAEAHGEPAGRASHRAIVTDKREAIMTA
                     ALELFVERGFFGTAVPEIADKAGVGAGTIYRYFESKEALVNALYRQEKQRFAERVIHD
                     FPTTTIARELFRTMWMRMATFAVENPKPFIFLELHHHASYLDAESRALEARMLDLFTN
                     VVVASQARGELKKGAPRLLMGMVMGAFVGVIRSCLEIDQPLADADWKLAEQCIWEAIR
                     A"
BASE COUNT          513 a          996 c          892 g          479 t
ORIGIN      
        1 gaaggcatca gcgagctgca caaggcgcag gatctcgagc cgctgcgctc gcgcgcgtac
       61 gtcgccgagg cgcgctggct ggcgtcgaag ggacgcaccg acgaggcgat cgcgaaggca
      121 cgcgaagcga cgatcgtcga gccgactgac caagaggcga gcagtctgct aaacagtctc
      181 gtcggcaaat gactgcggtg gccgccacga ctgactcgct cgcgccgcgc gatcgccgcg
      241 cgcgcgccac gaagatccat tcgatcctcg acgagctgtt ccccggtccc gcgatcccgc
      301 tcgatcacgg cgatccgttc cagctgctcg tcgcgacgat cctgtcggcg caatgcaccg
      361 atgcacgcgt caacatggtg acgcccgcgc tgttcaagcg cgcacccgac gcgcgcacga
      421 tggcgaagat gacggccgcc cagatcctgc cgttgatccg cacgtgcggg ctcgcgccag
      481 gcaaagcgaa gagcctcgcc gcgatgagcc gtgcgctcgt cgccgatcac ggtggtgacg
      541 tgccgggtga cttcgacgcg ctcgagaatc tcgcaggcgt cgggcacaaa acggcaagcg
      601 tcgtcatggc gcaggcgttc aacgtgcctg catttccggt cgacacccac atccatcgcc
      661 tcgccgctcg ctggcagctc tcgcgtgcgc gcaccgtcga ggaaaccgag cgcgatctca
      721 aggccgtgtt cccgcgggag gcgtggaacg tgttgcatct ccagatcatc tacttcggcc
      781 gtacgtactg ccccgcgcgc ggccacgcgc ccgagacgtg tccgatctgc agctgggcga
      841 tgtcgaaggc gcgcgccgca cgtgaggccc gcgcgcaacg ccggtcgtga cgcgctgagt
      901 ccggcaatct tcggacccat ttgggtcacg gcgaacgtcc aacgggaatg ctacgtctga
      961 tcgagatgcg tcgtccgctc ctcgtcgcgg tggtcgttct atcgggttgc tggaagctgc
     1021 aaggcattcc gaccaaccac cagctcgaag caacgttccc ggcgagcgtg cagacaaccg
     1081 acctgcccgg gcagttcaag gtcgctacgt tcaacattca catggagccg gggcacaagg
     1141 tcgccacggc cctgctcgct gatcgcgcgc tgcgcgatgt cgacgtgatc gcgctcgaag
     1201 aagtgccgcg cgagaacggc tcgacctgca gctcggcgtg cgcgatgggt cacgagctcg
     1261 gcatgtactc ggtgttcgca ccggcccact cgaaggacgg cgacgatttc ggcgttgcga
     1321 ttctgtcgcg cgtgccgatc acgtcggcgc gcgtcatcga gctgccgtgg aacgacgtcc
     1381 acttcaatgc cggcaggcgc atcgcgctcg tcgcgaccgt gcagcatgaa ggccggccga
     1441 tcacggtcta cgccgtgcac ctcgagaacc ggctcaccgc gagcgatcgc aagaggcaga
     1501 tgaagccggt gctcgtggac gcgagccaac aacgcacgcc agtcatcatc gcgggcgact
     1561 tcaatacgaa cccgttcacc tggatcagcc attggatccc gctgccgacc ggcgcacatc
     1621 agcagcacgc gctcgaatcg ttcgtgcgct cgtacggctt cgacacgccg gtcgccggca
     1681 gcggcgcgac tcaccgttac atcggcatga agctcgacgc gatctacacg cgcggcttcg
     1741 acaccaagca cttcgccgtc gcgcacgcca aggacgtctc cgatcacatg gcgctgtggg
     1801 cgatcgtcgc gccgaccccg cagccgccga ccgtcgcggc cgcgaaatag gggtctgacc
     1861 ggtctgaccc ctttttccgc gcctcgcttc agaaaggggt ctgaccggtc tgaccccttt
     1921 tatttgcgcc gacggtacaa gcacttgcga aataactccg agaaactgct tgcgttggcc
     1981 gcgcggaatg aatattcatt ctctcgcgat gacgacgaca gccaagaccg aagcggcccg
     2041 ccgaggcgcc gtcgacaagg ccgaagcgca tggcgagccg gccggccgcg ccagccatcg
     2101 cgcgatcgtc accgataagc gcgaggcgat catgaccgcg gccctcgagc tgttcgtcga
     2161 gcgcggcttc ttcggcacgg cggtccccga gatcgccgac aaggccggcg tcggcgctgg
     2221 caccatttat cgctacttcg agtcgaagga agcgctcgtc aacgcgctct accgccagga
     2281 gaagcagcgg ttcgccgagc gcgtgatcca cgattttccg acgacgacga tcgctcgcga
     2341 gctgttccgc accatgtgga tgcggatggc gacgttcgcc gtcgagaacc ccaagccatt
     2401 catcttcctc gagctgcacc atcacgcgtc gtatctcgat gccgagagcc gcgcgctcga
     2461 agcacgcatg ctcgacctgt tcacgaatgt cgtcgtcgcc tcccaggcgc gcggcgagct
     2521 caagaagggc gcacctcgcc tcctcatggg gatggtcatg ggtgcgttcg tcggtgtgat
     2581 ccgcagctgc ctcgagatcg accagccgct cgcggacgcc gactggaagc tcgctgagca
     2641 gtgcatctgg gaagcgatcc gcgcgtaacc ctcgtcctcc gcggcttagc ccgctttttt
     2701 atttgtcttt agtcggaatg aacgttcatt ccaagaaccc tcaggagacc tcccatgtcg
     2761 aactcgtcca agaccgttct catcactggc gccaccgcgg gcatcggccg catgaccgcg
     2821 ctccacctgg ccaagctcgg ccatcacgtc atcgccaccg gtcgcaagcc ggccgagctc
//
LOCUS       DASUFX010000647         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_102756,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000647 DASUFX010000000
VERSION     DASUFX010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102756"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFV99_26295"
     CDS             <1..1015
                     /locus_tag="VFV99_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062923.1"
                     /translation="PVQQGYTAADRARVLWAGLPTDSGPMKLAGDTYAVHYFRYVFGF
                     DMAGEIVWAYSHPRVELVASDHTGNAIIAVSTNGDLVVLDPKTGAVRQRKNLGLGQQV
                     VGATFDADGWSPPQGDAEKVETVVALVSIARDRDARFDRVKELAVSALAKLPGGEVTK
                     ELLSVLGDKRAPQKLKDTVVEMLAQRKDPAGLPVLTDQLAIHTDYIAKTEAESLGAVA
                     KAIASLAGTKLDAKEVTPALAALQGHLDDPATQTPDLIMVIRAMAAIGGGAERPVLAS
                     HLLLYHADDEVGADAAWQTAIVTALDVHGGPGEHELLRQVAADPRTKQGLAAAIKDAL
                     VND"
     gene            1448..2026
                     /locus_tag="VFV99_26300"
     CDS             1448..2026
                     /locus_tag="VFV99_26300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062924.1"
                     /translation="MARNTIAIFIFALGFALGCKGETVVKENPDTLKDLDVCKKTLAE
                     KEKLAQALQDENARLMRGSGASEIVVAIEGNLFTVRPGKPGEARPIDDKVAAAQSKEF
                     YDLVAKSRGAIQKCYEQVLKKDTNLQARTVTLTVSATFSNAGAYQNSSFHATQDLGDT
                     FDNCMKTIAQKWQLPQNAQTMTFKAQVSLTPS"
     gene            2023..>3278
                     /locus_tag="VFV99_26305"
     CDS             2023..>3278
                     /locus_tag="VFV99_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062925.1"
                     /translation="MKLKDAEKRLKNEPNNLGLRVQVAGLMREAGRSIEAVELYRSVA
                     LAYRDQGRTQQAIAVCKSILEIAPEDSACQGLLAMLQAKAIMPAAPPARPSRPSRPPA
                     NASRPPANEPVKRPIVADPVKPPPQSVVMRGSPMPPAPEVPSLRSPTSAPATRSPTSP
                     PGTKPIAVGQRPLTPTRPPSRGMEAAEQPTRDRPPTRPQVPMPAKPAGDAARTQPMPL
                     KPPAQHDQRVSDPGRRSSFDATPLPPPMPYHQADRTSQPTKVSMRDLDSDATRVDPNP
                     GLAQAARRISGLISELEEGVPNEMDLAAELETRQRPRIASDELNKIAAPPPTTTPIDR
                     LDEIIDDLTTPPPADYDTLATDRRRALTPHPRGSDDAITTPPERTSTPSNPTITSTSE
                     RNLPRVGPPQRTSSRPPAVVSIPPKT"
BASE COUNT          594 a         1109 c         1084 g          491 t
ORIGIN      
        1 tccggtccag cagggctaca cggcggccga tcgcgcacgc gtgctctggg cggggctgcc
       61 caccgactcc ggtccgatga agctcgcggg cgacacgtac gcggtgcact acttccggta
      121 cgtcttcggc ttcgacatgg cgggcgagat cgtgtgggcg tactcgcatc cgcgtgtcga
      181 gctcgttgcg agcgatcaca ccggcaacgc gatcatcgcg gtgtcgacga acggtgacct
      241 cgtcgtcctc gatccgaaga ccggcgcggt gcgccagcgc aagaacctcg gcctcggtca
      301 gcaagtcgtc ggcgcgacgt tcgacgctga cggttggtca ccgccgcaag gtgatgcaga
      361 gaaggtcgag accgtcgtcg cgctggtctc gatcgcgcgc gatcgcgatg cacgcttcga
      421 tcgcgtgaag gagctcgccg tgtccgcgct cgcgaagcta cccggtggcg aagtgacgaa
      481 ggagctgctc tcggtgctcg gcgacaagcg cgcaccgcag aagctcaagg acaccgtcgt
      541 cgagatgctc gcgcagcgca aggaccccgc cgggttgccg gtgctcacgg atcaactcgc
      601 gattcacacc gactacatcg cgaagacgga ggccgagtcg ctcggtgccg tcgcgaaggc
      661 gatcgcgagc cttgccggca ccaagctcga cgccaaggag gtcacgccgg cgctggccgc
      721 gctccagggg catctcgacg accccgcgac acagacgccc gatctgatca tggtgatccg
      781 cgcgatggcc gcgatcggcg ggggcgccga acggccggtg ctcgcgtcgc atttgctgct
      841 gtatcacgcg gacgacgagg tgggcgcgga cgccgcctgg cagaccgcca tcgtgaccgc
      901 gctcgacgta cacggtgggc ctggcgagca cgaattgctg cgtcaggttg ccgctgatcc
      961 gcgtaccaag cagggcctgg cagctgcgat caaggacgcg ctggtcaacg actaagcacc
     1021 caaggtacgc tcctggttcg gtggccacgc cacgtcgttt caagatcgtc gtctgccggg
     1081 ggcctgagtg cggcgatcga cgtgggtcgg cggcgctcca cgaggtgttc cgatctgcgc
     1141 tcgaggcaca cggcgcgcgc gatcgcgcag atctgatgtg gcagagctgc tttggtcgct
     1201 gcacgcaggg gcccaacgtc ctggtgcgcg agctcgtcgc ggaacaagcg ccatcgttgg
     1261 gtagcgggtt tgccacgctg ccgggacctc ggggcgcgac agcgctgtat aatcggatgg
     1321 atccggaacg agtagagcgc gtgattgttc aacacgtgat tggtggccag atcgttcgcg
     1381 agctcatcga gcggcctggc atcctccact caacgcccgt aggatctagg acgaaagaca
     1441 gttagccatg gcgcgcaata cgatcgcgat cttcatcttt gccctcggct ttgctctcgg
     1501 ttgcaaaggc gaaaccgtcg tcaaagagaa tcccgacacg ctcaaggatc tcgacgtttg
     1561 caagaagacg ctggccgaga aggagaagct cgcgcaggcg ctgcaggacg agaatgctcg
     1621 cttgatgcgc ggctcggggg cgtccgagat cgtcgtcgcc atcgaaggca atctgttcac
     1681 cgtgcgcccc ggcaagcccg gcgaggcgcg cccgatcgac gacaaggtcg cggctgcaca
     1741 gtcgaaagag ttctatgatc tcgtcgcgaa atcgcgtggc gcgatccaga agtgctacga
     1801 gcaggtgctc aagaaggaca cgaacctgca agctcgcacg gtcacgctga ccgtgtcggc
     1861 tacgttctcg aatgccggtg cctatcagaa ctcatcattt catgcgaccc aggacctggg
     1921 tgacacgttc gacaactgca tgaagacgat cgcgcagaag tggcagctgc cgcagaacgc
     1981 gcaaacgatg acgtttaagg cccaggtgtc cctgacaccg tcgtgaagct gaaagacgcc
     2041 gagaaacggc tcaagaacga gccgaacaat ctcgggcttc gcgtccaggt cgctggcctc
     2101 atgcgcgaag ccgggcgcag catcgaagcg gtcgagcttt atcgctcggt cgcgctcgcg
     2161 tatcgcgatc aaggtcgcac gcagcaggcg attgcggtct gcaagagcat cctcgagatc
     2221 gcgcccgaag actccgcttg tcagggcttg ctcgcgatgc tccaggcgaa agccatcatg
     2281 cctgcggcgc cgccggcccg tccgtcacgt ccatcgcgac cgcccgcaaa cgcgtcgcga
     2341 ccacccgcaa acgagccggt aaagcggccc atcgttgctg accccgtaaa gccaccgccg
     2401 cagtcggtcg tgatgcgcgg ctcgccgatg ccgccggcgc ccgaggtgcc gtcgctgcgc
     2461 tcgccgacgt cggcgcctgc gacgcgctcg ccgacgtcgc cgccgggtac gaagccgatt
     2521 gcagtcggcc agcgtccgct gacgccgacg cgtccaccgt cgcgcggcat ggaagcggcc
     2581 gagcagccga cccgcgatcg gccgccgacg aggccccagg ttccgatgcc ggccaagccc
     2641 gccggtgatg cggcgcggac gcagccgatg ccgctcaagc cgccggcgca acacgaccaa
     2701 cgcgtgtcgg atccggggcg tcgctcgtcg ttcgatgcga cgccgctgcc gccgccgatg
     2761 ccgtatcacc aggccgatcg gacgtcgcaa ccgacgaagg tctcgatgcg cgatctcgat
     2821 tccgacgcca cgcgggtgga tccgaatccc ggcctcgcac aggccgcgcg tcggatctcc
     2881 ggcctgatca gcgaattgga agagggcgtg ccgaacgaga tggatctggc agccgagctc
     2941 gagacgcgcc agcgcccgcg gatcgcatcc gacgagctca acaagatcgc ggcaccgccg
     3001 ccgacgacga cgccgatcga tcggctcgac gagatcatcg acgacctgac gacgccaccg
     3061 ccggccgact acgacacgct cgctaccgat cgccggcgcg cgctcacgcc gcatcctcgc
     3121 ggcagcgacg acgcgatcac gacgcccccc gagcgcacga gcacgccgag caatccgacg
     3181 atcacgtcga cgtcggagcg gaacctgccg cgcgtcgggc cgccgcagcg cacgtcgtca
     3241 cgtccgccag cggtcgtgtc gatcccgccg aagacgca
//
LOCUS       DASUFX010000648         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_658685,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000648 DASUFX010000000
VERSION     DASUFX010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658685"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFV99_26310"
     CDS             <1..825
                     /locus_tag="VFV99_26310"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5062926.1"
                     /translation="AKQSPPAVQMQKAPASPSKSRAPLFALIVLLLGGLGAGGYFAYM
                     KMNEKKPEVAANGTGKTGSNGSAAVKTATPDAAVAVAKVEADAAVAVPTAAPDAAQVA
                     EAKIDAAVVATATPDAAVEKPTEVVKPTGASDKLQISSKPAGARVFIDGADQGVTPLK
                     LAGSADRHTMVLLEPGHDLYIGEVDGHGVFDIALKPITPTGGNAGIKVIKCKAKERYY
                     VYVDGAPTGMTCPTERIDTVVGAHTVEVYDIVTDSRRKFDIFVKDERLSARVRIDD"
     gene            complement(876..1256)
                     /locus_tag="VFV99_26315"
     CDS             complement(876..1256)
                     /locus_tag="VFV99_26315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062927.1"
                     /translation="MRPLQLLIAALLGASIVLAILLATITRECELVALAPMPMPVQTI
                     DTDCYSCYSGSGPSAWELAGLVDLADDERIVAVDRKPTTPSKQFMLVELIDAAYGRMR
                     SHGQAMEFDIAGPRGQRRVELVLH"
     gene            1315..2469
                     /locus_tag="VFV99_26320"
     CDS             1315..2469
                     /locus_tag="VFV99_26320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062928.1"
                     /translation="MRWIALVLVVAACSKGRPDDCAIVRDKPESAMAELSSRYPNNPV
                     KVAETIEKCVAPSGEECDRIAKIVTAIPTLAPQLSLPKPFDYAEVCRHSPPELRRCLL
                     PSYSLGHTLECQTEMKKTLTQLEIKPKNPEHPEGDTYPDDCAGFVSIYITKDGTWLAT
                     GRDDKARCFAPRKPAAPSATGELDADWLELQLRKLKATDCPPSSAELASAPDVAYRDV
                     IATMDVSIKAGVPDVGLSEPDKLSVPLATANPKGAPSDCPPAVLHLADNAPGDHSSPT
                     RDHAMPPDNQGPPSTNTKDALASAPVLVITKDALSLSAGSTKTELGSIADASKGTGNF
                     VALTKALPPTKNGLLILQADASTPIAVINRVVSTAKAAGYTNVMFAVKKK"
     gene            complement(2472..2774)
                     /locus_tag="VFV99_26325"
     CDS             complement(2472..2774)
                     /locus_tag="VFV99_26325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062929.1"
                     /translation="MEPWLIGAIAGGTAALAVMVTARRGNKTALEILPLLRDRGPLTI
                     PEIMEQLGLVGFSAQGKVVMALDSLIRSGNVEERPVPPGTPQLEKIKVRKYAVRAS"
     gene            complement(3132..4466)
                     /locus_tag="VFV99_26330"
     CDS             complement(3132..4466)
                     /locus_tag="VFV99_26330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5062930.1"
                     /translation="MDDAPSYSRQNAGGIFVVIKGPDRGEAVRLTSEPVSFGSGPQCS
                     LVLTDKTVSRKHLEASLVGDEVVMADCGSTNGTFIQGSRFERISIGFGAEVKLGRTVL
                     KFLPDEEIVEPEPSAQAAFGTIVGGDTKMRQMFTLLEDVAATNATVLIEGETGTGKEL
                     IAEEIHSHSPRRDGPFVVFDCGSVPRELIESMLFGHVKGSFTGAITDRRGAFAEAHGG
                     TIFLDEIGEMALDLQPSLLRVLDKRAVRKVGSNTYEKIDVRVVAATNRDLRAEVAKKT
                     FREDLYYRLAVIRVSVPPLRERGTDIPLLIQHFVNSFGQGLSVAPEDMARLVRHSWPG
                     NVRELRNVIERACLLARGSSINLEDALVSEAAPSLGIRTDLPFKEAKGQLVERFEQEY
                     IEDLMRRHKMNLSAAAREAQIDRKHLRELIRKYGLDPRHQGGEGGGVDEDLD"
     gene            4646..>4834
                     /locus_tag="VFV99_26335"
     CDS             4646..>4834
                     /locus_tag="VFV99_26335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062931.1"
                     /translation="MIKPITSQGVNPDRSARVDAFRRPVSGTERRRPCCAGCIGICCI
                     GICCIGICCIGICCIGICC"
BASE COUNT          873 a         1618 c         1531 g          812 t
ORIGIN      
        1 gcgaagcaat cgccgccagc ggtgcagatg caaaaggcac cggcgtcgcc gagcaagtcg
       61 cgcgccccgc tgttcgcgct catcgtgctc ttgctcggtg gcctcggcgc gggcggctac
      121 ttcgcgtaca tgaagatgaa cgagaagaag ccggaggtcg ccgcgaacgg aaccggcaag
      181 accggcagca acgggtcggc cgccgtgaag acggcgacgc ccgacgcagc cgtcgcagtc
      241 gcgaaggtcg aagccgatgc ggcggtcgca gtgccgacgg ccgcgcccga cgctgcacag
      301 gtcgccgagg cgaagatcga tgccgcggtc gttgcaacgg cgacgccgga tgcggccgtc
      361 gagaagccga ccgaggtcgt caagccgacc ggcgcgtcgg acaagttgca aatctcgtcg
      421 aagcccgccg gtgcgcgcgt gttcatcgac ggtgccgatc aaggcgtcac gccactcaag
      481 ctcgccggca gcgcggatcg ccacacgatg gtgctactcg agcccggcca cgatctctac
      541 atcggcgaag tcgacggcca cggcgtgttc gacatcgcgc taaagccgat cacgccgacc
      601 ggcggcaatg ccggcatcaa ggtgatcaag tgcaaggcga aggagcgcta ctacgtctac
      661 gtcgacggcg cgccgaccgg catgacctgc cccaccgagc gtatcgacac cgtcgtcggt
      721 gcgcacaccg tcgaggtcta cgacatcgtc acggactcgc gccgcaagtt cgacatcttt
      781 gttaaagacg agcgcctctc cgcgcgcgtg cgcatcgacg actgatcggg cgtcggacct
      841 cggactacag gcttcgggtc agaatccgat ccggatcagt ggagcacgag ctcgacgcgg
      901 cgctgaccgc gcggccccgc gatgtcgaat tccatcgctt gcccgtggct gcgcatgcgg
      961 ccgtacgctg catcgatcag ctcgacgagc atgaactgct tgctgggtgt cgtcggtttg
     1021 cgatcgacgg cgacgatgcg ctcgtcatcg gcgaggtcca ccaagcctgc aagctcccac
     1081 gcactcgggc cgctgcccga gtagcacgag taacagtctg tatcgatggt ctgcaccggc
     1141 atcggcatcg gcgcgagggc gaccagctcg cactcgcgcg ttatcgtcgc gagcaagatc
     1201 gccagcacga tggacgcgcc gagtaacgcc gcgatcagca gttgcagcgg tcgcatccca
     1261 tgaacgtgtc acacattcat ccgcgtgcac gcgatcattc gttacagtcg cgtgatgcgt
     1321 tggatagcgt tggtgctcgt cgtcgctgcg tgcagcaaag gtcggccgga tgattgcgcg
     1381 atcgtgcgcg acaagccgga gagtgcgatg gcggagctca gctcgcgcta tccgaacaat
     1441 ccggtgaagg tcgccgagac gatcgagaag tgcgtcgcac cgagcggcga ggagtgcgat
     1501 cggatcgcga agatcgtgac cgcgatcccg acactggcgc cccagctctc gctgccgaaa
     1561 ccgttcgact acgcggaggt ctgtcgccac tcaccgccgg aattacgacg ctgcttgctg
     1621 ccgagctatt cgctcggaca cacgctcgag tgccagaccg agatgaagaa gacgctgacg
     1681 caactagaga tcaagccgaa gaacccagag catccggaag gcgacacgta tcccgacgac
     1741 tgcgctggct tcgtgtcgat ctacatcacg aaggacggca cgtggctcgc gaccggccgc
     1801 gacgacaaag cacgttgctt cgcgccgcgc aaaccggccg cgccttctgc gaccggcgag
     1861 ctcgacgccg actggctcga gctgcagctg cgcaagttga aggcgaccga ctgcccgcca
     1921 tcgtcggcgg agctagcgag cgcgcccgat gtcgcgtatc gcgatgtgat cgcgacgatg
     1981 gatgtctcga tcaaagctgg cgtgcctgac gttggcctgt cggagccgga caagctctcg
     2041 gtgccactcg cgactgcgaa cccgaagggc gctcccagcg actgcccacc tgccgttctc
     2101 catctcgccg acaatgcgcc gggagatcac agctcgccta cgcgcgacca tgccatgccg
     2161 cccgataacc agggcccgcc gtcaacaaac accaaggatg cgctcgcgag tgcgccggta
     2221 ctcgtcatca cgaaagatgc gctgagcctg agcgccggca gcacgaagac ggagctcggg
     2281 tcgatcgccg acgcaagcaa aggcaccggc aacttcgtcg cgctgaccaa ggcgctgccg
     2341 ccgacgaaga acggcttgct catcctgcag gccgacgcga gcacgcccat cgcggtcatc
     2401 aaccgcgtgg tctcgaccgc caaggcagcc ggctacacca acgtcatgtt tgcggtgaag
     2461 aagaagtaac gtcagctcgc gcgcaccgcg tacttgcgga ccttgatctt ctcgagctgc
     2521 ggtgtgcccg gcggcaccgg acgctcctcg acgttgcccg aacgaatcaa cgagtcgagc
     2581 gccatcacga ctttgccctg tgccgagaac cccacgaggc cgagctgttc catgatctcc
     2641 ggaatcgtca gcggtccgcg gtcgcgcaag agcggcagga tttcgagagc cgtcttgttg
     2701 cctcgacgcg cggtcaccat cactgcgagt gcggcggtac cacccgcgat tgcgccgatg
     2761 agccagggtt ccatggccgt agtgcaatag cagggttggt gccgcaactc gcgcccatgg
     2821 tttcgccacc ttagccagcc gtgcaccagg tgcactgcag gtgcagcgtg gcccgaccct
     2881 gcctgtcgcg cctacgtacc ggggttcgtt ttcggcccga ccttacgtac cggggttagt
     2941 ttttcgtccg acctgcggcc agcgcgtccg acagccagcc gaccttacgt accggggtta
     3001 gtttcgcccc gacctttacg taccggggtt agcttttcgg cccgacctta cgtaccgggg
     3061 ttagtttttc gtccgacctg cggccggtgc gtccgacagc cagccagcgc gcgtccgaac
     3121 cgcggccagt gctagtccag atcttcgtcg acgccaccgc cctcgccacc ttgatggcgc
     3181 gggtcgaggc cgtattttcg gatcagctcg cgcaggtgct tccgatcgat ctgcgcttcg
     3241 cgcgccgcgg ccgacaagtt catcttgtgc cggcgcatca agtcttcgat gtactcctgc
     3301 tcgaagcgct cgacgagctg acccttggct tccttgaacg gcaggtcggt gcggatgccg
     3361 aggctcggcg ccgcttcgct gaccagcgcg tcttcgagat tgatcgacga gccgcgcgcg
     3421 agcagacacg cgcgttcgat cacgttgcga agctcgcgca cgttgcccgg ccagctgtgg
     3481 cgaacgaggc gcgccatatc ctcgggcgcg accgagagac cctggccaaa tgaattcacg
     3541 aagtgctgga tgagcagcgg aatgtcagtg ccgcgctcgc gcaacggtgg cacgctcacg
     3601 cggatcaccg cgaggcggta gtagaggtct tcgcggaatg ttttcttcgc gacttcggcg
     3661 cgcagatcgc ggttcgtcgc agcgacgacg cgcacgtcga tcttctcgta cgtgttgctg
     3721 ccgaccttac gcaccgcgcg cttgtcgagc acgcgcaaca gcgacggttg cagatcgagc
     3781 gccatctcgc cgatctcgtc gaggaagatc gtgccgccgt gcgcctcggc gaatgcgccg
     3841 cgccgatcgg tgatcgcgcc ggtgaagctg cccttcacgt gaccgaacag catcgactcg
     3901 atcagctcgc gcggaacgct accgcagtcg aacacgacga acgggccatc gcggcgcggc
     3961 gagtggctgt ggatctcttc ggcgatcagc tccttgccgg tgccggtctc gccttcgatc
     4021 agcacggtcg cgttcgtcgc ggcgacgtcc tcgagcagcg tgaacatctg ccgcatcttg
     4081 gtgtcgccac cgacgatcgt gccgaacgca gcctgcgcac tcggttcggg ctcgacgatc
     4141 tcctcgtcgg gcaagaactt gagcacggtc cggccgagct tcacttcggc gccgaagccg
     4201 atcgagatgc gctcgaaccg cgagccctgg atgaacgtgc cgttcgtcga gccgcaatcg
     4261 gccatcacga cctcgtcgcc gaccagcgat gcttcgaggt gcttgcgcga caccgtcttg
     4321 tcggtgagca cgagactgca ttgcgggccc gagccgaacg agaccggctc gctggtcaac
     4381 cggaccgctt cgccgcgatc gggacccttg atgacaacga agatcccgcc cgcgttttgc
     4441 cgcgagtacg aaggagcgtc gtccaacagc gcggtgacgt tagaacggtc gcgagacatc
     4501 cggcagccag cacatagtag tcctacgcgg ctcctggtgt ctagaccccc acccctagag
     4561 atctagtaag gatcgcgcct actttccggc cggcaagctc ggaccggaca tcgcgacgat
     4621 caaagtaacg acgattgcga cgacgatgat caagccgatc acgagccagg gagtgaaccc
     4681 ggatcgctcc gcgcgggtcg acgcctttcg ccggccggtg tctggaactg aacgccgccg
     4741 cccctgctgc gcgggctgca tcggcatctg ctgcatcggc atctgctgca tcggcatctg
     4801 ctgcatcggc atctgctgca tcggcatctg ctgc
//
LOCUS       DASUFX010000649         5992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1771379, whole genome shotgun sequence.
ACCESSION   DASUFX010000649 DASUFX010000000
VERSION     DASUFX010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771379"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV99_26340"
     CDS             <1..225
                     /locus_tag="VFV99_26340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062932.1"
                     /translation="VLSKQTDGVILVAHADKTLRDELKRSAKQIKAVDGKIFGVIVNA
                     IEPDQRSGYYYSYYGYTYSEKSPEPSSTAS"
     gene            225..950
                     /locus_tag="VFV99_26345"
     CDS             225..950
                     /locus_tag="VFV99_26345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5062933.1"
                     /translation="MVTATLKTIAKRARSQVERYIYRGDAVNCIVCGYRARSWLRGQT
                     YGRCPNCRCASRTRVLWRYLERTYTSPQQLKVLHFAPEAPLRARLTAWGAASYTTCDY
                     NRSDVDCRVDIQKLRFADGAYDLVLCSHVLEHIPDHGAAMRELHRICSPNGHVLIQVP
                     LDRAMPTREDLTEMPVSERKRLFGEIDHLRYYGSDFAELLKRAGFSRVTTHEPVREIV
                     ITERIEYGFNPEEALFVCQRSAL"
     gene            complement(939..1739)
                     /locus_tag="VFV99_26350"
     CDS             complement(939..1739)
                     /locus_tag="VFV99_26350"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5062934.1"
                     /translation="MSSPWATRLDVATAAVRAAGAALMQLRGTIRGEEAAGGQLKTST
                     DLAAEGWVCGFLEGTFPGELFLAEERFERVGTPWPGASSYWAVDALDGTRSYVDGYDG
                     FCVQVAYIDGGQPRVGVIGEPVTGAVYVAASGAGAWRLDDAGARELRASSAVALVPGL
                     RFVDSTTPSGPVGAIFQQVKGRFVECGSVGLKICRVAEDAADVYAKQFNYKLWDVAPG
                     EVLLREVGAVLTTYSGETIDYAGARTHFPNLVVARPDLATAIIGELQR"
     gene            complement(1747..2640)
                     /locus_tag="VFV99_26355"
     CDS             complement(1747..2640)
                     /locus_tag="VFV99_26355"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5062935.1"
                     /translation="MLVTGATGFLGRRVVARLVERGIEVTAHGNSRAFRDLGEVRLDK
                     RFDAVESMQGDLADYDIATDLLGSWRWNAVVNLAGPVTSGNEDLRTGIAVVTAHERIA
                     LHVKRFAEDARIVHASSMTVYGVPEQVDVDEYHPTRPVHLYGLAKLVAECILLVDPAT
                     DAWVLRLPGLFSEDRASGALFHFCRAARAGEPIRVTTATPTAWNVLHVDDAADAIVRA
                     LTATGKPRTAINISYDEPVDLIAVAKLIAAHAGAGSTVEAAVAHPPFRMNATRAKQSL
                     GWKPPTLRERLTKLYADYAAT"
     gene            complement(2646..3632)
                     /locus_tag="VFV99_26360"
     CDS             complement(2646..3632)
                     /locus_tag="VFV99_26360"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5062936.1"
                     /translation="MPTPIKLFFTGGGGAGTIEAVKVLKALGYEVVTADATPTSAGFS
                     WADRSYVIPFGADDAFVPALREIVRRERPDFIIPLVDEEIPKVHRFVREEVPTLKIVG
                     PTLEFSELTLDKWTMAQALTRHGLSVARSWLASDAAAATYPAIVKPCQGRGSRGLAFL
                     DGPGDLRAYLAAAAQPADRYIVQERLHGREFTTSVVVGLDGTLFSIVPKEAADKRGIT
                     QVGITRSVPAIDELCRGITKALDPRGPYNVQLIVGDDGVPCVIEINPRYSTTMALTLA
                     SGVNEVDVVLRHARGEDPGQLTFTPDLMMLRYTAQIYVKESEWRPIDLRAKP"
     gene            3688..4326
                     /locus_tag="VFV99_26365"
     CDS             3688..4326
                     /locus_tag="VFV99_26365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5062937.1"
                     /translation="MPRGPATFYNRFAKRWFDIAASATALVALGPLIGAVSLAVLRKH
                     GRPILFRQQRTGVNGEPFEILKFRTMTDERDASGNLLPDKMRLTALGKWLRETSIDEL
                     PELLNVLRGEMSLVGPRPLLHRYMRHYNERQHRRHEARPGITGWVAVNGRNTTTWEQR
                     FEMDVYYVENLGPLLDMKILLRTIVTVLSREGVDPGFADQMPEFGTPAPATN"
     gene            complement(4328..5476)
                     /locus_tag="VFV99_26370"
     CDS             complement(4328..5476)
                     /locus_tag="VFV99_26370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5062938.1"
                     /translation="MTVPVTANVLLRGQLAYLSEHGFEITVISSPGPELERVAEREQV
                     DVVGVPIAREIDPAQDAISLARVTRALGQLKPDIVNASTAKGGLIGIVAAAALRVPVR
                     IYQLRGLRLETERGFKRSVLGMTERTAAACAHEVVCNSESLRAAYVAARYAPASKCVV
                     LGAGSSNGVDVERFARARWTDQAQTLRRELAIPNNANVIGFIGRPVADKGIAELLAAF
                     ERVRVRAPNTHLVIVGAGFAGDIVDPAFAAQLRGDNVHLVARVDEPAPYYAMMDVLAF
                     PSHREGFPNVPLEAAAAGVPTVGARATGITDAVRDGNTGLLVDVGDVGGLTHALLRYL
                     LEPKLRDAHARAARHRAVDYFARDVVWAGWRDEYWRLLRVRGLPDEHA"
     gene            complement(5503..>5992)
                     /locus_tag="VFV99_26375"
     CDS             complement(5503..>5992)
                     /locus_tag="VFV99_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062939.1"
                     /translation="IFEASKLPYVRGMCPPGWNAPPALLTAMNELGFDFVASSRDIKT
                     EISAAATTNMSGIKGASLIYPSLVGGTNLVHFATNFQATSQWERAKAIIEAGGLLTVK
                     AHIIKNALGYIALDGVDGVYCNFLDLFFERLHREYGDSLWWTTMGEIAKRLRAQPAEK
                     AS"
BASE COUNT         1103 a         1987 c         1878 g         1024 t
ORIGIN      
        1 gtgctgtcga agcagaccga tggcgtgatt ctcgttgcgc acgctgacaa gacgctgcgt
       61 gacgagctca agcgctcggc aaaacagatc aaggctgtcg acggcaagat cttcggcgtt
      121 atcgtaaacg cgatcgagcc cgatcagcgc agtggctact actactcata ttacggctac
      181 acgtactccg agaagtctcc cgaaccctcg tcgactgcgt cgtgatggtc accgcgactc
      241 tcaagacgat cgcgaagcgc gctcgcagcc aagtcgagcg ctacatatat cgtggcgacg
      301 cggtgaactg catcgtgtgc ggctaccgag cgcgctcgtg gcttcgcgga caaacgtacg
      361 ggcggtgccc gaactgtcgc tgcgcgtcac gaacgcgcgt gctctggcgg tatctcgagc
      421 gtacctatac gtcaccacag cagctcaaag ttctgcactt tgcgccggag gcgccgctgc
      481 gcgctcggct gaccgcgtgg ggtgccgcgt catacacgac ctgtgactac aaccggtctg
      541 atgtcgactg tcgcgtcgac atccagaagt tgcgattcgc agacggtgcc tatgatctcg
      601 tgctgtgctc gcacgtcctc gagcacattc cggatcatgg cgctgcaatg cgcgagctcc
      661 accggatctg ctcgccaaac gggcacgtcc tgatccaggt gccgctcgac cgcgcaatgc
      721 cgactcgcga agatctgacg gaaatgcccg tctcggagcg caagcgcctc ttcggcgaga
      781 tcgatcacct ccgctactac ggctcggact tcgcggagct actgaagcgt gcagggttct
      841 ctcgggtcac gacgcacgag cccgtgcgcg agatcgtgat caccgagcgc atcgagtacg
      901 gcttcaatcc cgaagaagca ctgttcgtct gccagcgctc agcgttgtag ctcgccaatg
      961 atcgccgtag ccaggtcggg acgcgcgacg accaggtttg gaaagtgcgt gcgcgcaccg
     1021 gcgtagtcga tcgtctcgcc actgtacgtc gtgaggaccg caccgacctc gcgcaacagc
     1081 acttcgccgg gcgcgacatc ccacagcttg tagttgaatt gcttcgcata cacgtcggcc
     1141 gcatcttcgg caacgcgaca gatcttgagc ccgacgctgc cacactcgac gaagcgtcct
     1201 tttacctgct gaaagatcgc accgaccggg ccgctcggcg tcgtgctgtc gacgaaccgc
     1261 aagccgggga cgagcgccac cgccgaggag gcccgcagct cacgcgcacc cgcatcgtcg
     1321 aggcgccagg cacccgcccc gctcgccgca acgtagactg cgccggtcac cggctcgccg
     1381 atgacaccaa cgcgaggctg accaccatcg atgtaagcga cttgcacgca gaacccgtcg
     1441 tagccatcga cgtagctgcg cgtgccgtcg agcgcatcga ctgcccaata cgacgaagca
     1501 ccaggccacg gtgtaccgac gcgctcgaac cgttcttctg cgaggaagag ctcaccgggg
     1561 aacgtgccct cgagaaagcc gcacacccaa ccttcagcgg cgaggtcggt gctcgtcttc
     1621 agctgaccac cggcagcttc ctcgccgcga atcgtgccgc gtagctgcat gagcgctgcg
     1681 cctgccgcgc gtacggcagc ggtcgcgacg tcgagccgcg tcgcccatgg cgagctcact
     1741 agtcgatcag gtagccgcat aatccgcgta gagtttcgtc agccgctcgc gcagcgttgg
     1801 tggtttccag cccaacgatt gcttcgcacg cgtggcgttc atccgaaacg gagggtgcgc
     1861 caccgccgct tcgacggtcg agcccgcacc agcgtgggcg gcgatcagct tcgcgaccgc
     1921 gatgagatct acaggctcgt catacgagat gttgatcgca gtgcgaggct tgccggtcgc
     1981 ggtcaacgcg cggacgatcg catcggccgc atcgtcgacg tgcagcacat tccacgcggt
     2041 cggcgtcgcg gtcgtcacac gaatcggctc gcctgcgcgt gctgctcgac agaagtgaaa
     2101 cagcgccccg ctcgcccgat cctccgagaa cagccccggc agccgcagaa cccacgcatc
     2161 cgtcgccggg tcgacgagca ggatacactc ggcgacgagc ttggcgagcc cgtacagatg
     2221 tacgggccgc gtcggatggt actcgtcgac gtcgacctgt tcgggaacgc cgtacaccgt
     2281 catcgacgag gcatgcacga tccgcgcgtc ttcagcgaat cgcttgacgt gcagtgcgat
     2341 gcgttcatga gctgtaacga cggcgatccc agtgcgcaga tcctcgttgc cgctggtgac
     2401 agggccggcg aggttgacga ctgcattcca gcgccacgag ccgagcaggt cggtcgcgat
     2461 gtcgtagtcg gcgagatcgc cctgcatcga ttcgacagcg tcgaaccgct tgtcgagccg
     2521 aacctcgccg aggtcgcgaa atgcgcggct gttgccgtgc gccgtgacct cgatgccgcg
     2581 ctcgacgagc cgtgcgacga cgcgtcggcc gagaaaaccg gtcgcaccgg tgacgagcac
     2641 gcgcttcaag gtttcgcccg cagatcgatc ggccgccact cactctcctt gacatagatc
     2701 tgcgccgtgt agcgcaacat catcagatcc ggcgtgaacg tcagctgtcc cggatcttcg
     2761 ccgcgggcgt gacggagcac gacatcgacc tcgttgaccc cggatgcgag cgtgagtgcc
     2821 atcgtggtcg agtagcgtgg attgatctcg atgacgcacg gcacgccatc atcgccgacg
     2881 attagctgga cgttgtacgg accacgggga tcgagtgctt tcgtgattcc acgacacagc
     2941 tcgtcgatcg ccggaacact gcgcgtgatg ccgacctgcg tgatcccgcg cttatcagcc
     3001 gcttccttcg gaacgattga aaataacgtg ccatcgagac cgacgacgac actcgtcgtg
     3061 aactcgcgcc cgtgcaggcg ttcctgcacg atgtagcgat ccgcgggttg tgcggcggct
     3121 gcgaggtacg cccgcagatc gccgggaccg tcgaggaatg cgagaccccg gctgccgcgt
     3181 ccttggcacg gcttgacgat cgccgggtag gttgccgcag cggcatcgct ggcgagccac
     3241 gagcgcgcca ctgacaagcc gtgccgcgtg agggcctgcg ccatcgtcca cttgtcgagc
     3301 gtgagctcgg agaactcgag cgtcggtccg acgatcttca gggttggcac ctcctcgcgc
     3361 acgaagcgat gcaccttcgg gatctcctcg tcgacgagcg ggatgatgaa gtcgggacgc
     3421 tcgcggcgaa cgatctcgcg cagcgcgggc acgaacgcgt cgtcggcgcc gaacggaatc
     3481 acgtacgagc ggtctgccca cgagaacccg gccgaggtcg gcgtggcatc ggcggtcacc
     3541 acctcgtagc caagggcctt gagcaccttg actgcttcga tcgtccccgc gccaccgccg
     3601 ccggtgaaga agagcttaat gggtgtgggc atgcggcctt cgtcctcgtt gccgtcgtcg
     3661 ggatgcgact gtaccttagc ggtagcgatg ccgcgcggtc cggccacctt ctacaaccga
     3721 ttcgcaaagc gatggttcga catcgcggcg tcggcgactg cgctagtggc cctcggcccg
     3781 ctcataggcg ccgtgtcact cgcggtgtta cgcaagcacg gccggccgat cctgttccgg
     3841 cagcagcgca ccggcgtgaa cggagaaccg ttcgagatct tgaagttccg caccatgaca
     3901 gacgagcggg acgcgagcgg taacctactg cctgacaaga tgcggctcac cgccctcggc
     3961 aagtggctgc gcgagaccag catcgacgag ctgcccgagc tgttgaacgt gctgcgcggc
     4021 gagatgagcc tcgtcggacc gcgaccactg cttcaccgct acatgcggca ctacaacgaa
     4081 cgccagcatc gccgccacga agcgcggccc gggatcacgg gttgggttgc ggtcaacggc
     4141 cgcaatacga cgacctggga gcagaggttc gagatggacg tctattacgt cgagaacctc
     4201 ggccctttgc tcgacatgaa gatcctactg cgcacgatcg tgacggtgct cagccgcgaa
     4261 ggcgtcgatc ctggttttgc cgatcaaatg cccgagttcg gcacgccggc accggcgacg
     4321 aactgattca tgcgtgctcg tccggaagcc cgcgcacccg tagcaagcgc caatactcgt
     4381 cgcgccagcc tgcccacacg acatcgcgag cgaagtagtc gacggcacga tgacgtgccg
     4441 cgcgcgcgtg tgcatcgcgc agcttcggtt cgagcaggta acgtaggagc gcatgggtga
     4501 gacctccgac gtcgccgacg tcgacgagaa gaccagtatt gccgtctcgc accgcatccg
     4561 tgatgccggt cgcccgtgcg ccgactgtcg gtacgccagc ggcggccgct tcgagcggca
     4621 cgttcggaaa accttcgcga tgcgatggga acgcgagcac gtccatcatc gcgtagtacg
     4681 gcgcgggttc atcgacacga gcaactagat gcacgttgtc gccgcgcaac tgtgcagcaa
     4741 atgccggatc gacgatatcg ccggcaaacc cggcgccgac gatcacgagg tgggtgttcg
     4801 gcgcgcggac gcgaacacgc tcgaacgcgg caagcagctc agcgatgccc ttgtctgcga
     4861 ccggccggcc gatgaagccg atcacatttg cattgttcgg gatcgcgagt tcgcgacgca
     4921 gcgtttgcgc ctggtcggtc caacgcgcgc gagcaaaccg ctcgacatcg acgccgttcg
     4981 acgaacccgc gccaagcacg acacacttgc tcgccggtgc gtagcgagct gcgacgtagg
     5041 ccgctcgcag actctcactg ttgcacacga cctcgtgtgc gcacgcggcc gccgtacgct
     5101 cggtcatccc gagaaccgac cgcttgaacc cgcgctcggt ctcgagccgc aggccgcgca
     5161 actggtagat gcgcacgggt acgcgtagcg ctgccgcagc cacgattccg atcagcccgc
     5221 ccttcgcagt gctcgcattg acgatgtccg gctttagttg gccgagcgcg cgcgtgacgc
     5281 gcgcgagcga gatcgcatcc tgtgcgggat cgatctcgcg tgcgatcggt acaccgacga
     5341 catcgacctg ctcgcgctcg gcaacgcgct cgagctctgg gcccggcgag ctgatgaccg
     5401 tgatttcgaa tccgtgctcg ctgagatacg cgagctgacc gcgcaacagc acattcgccg
     5461 tcaccgggac ggtcacgacg aacgcgagac gcggtcgccg gctcaggagg ctttctccgc
     5521 tggctgcgcg cggagacgct tcgcgatctc gcccatcgtc gtccaccaca gcgaatcgcc
     5581 gtattcgcga tgcagtcgct cgaagaatag atcgagaaaa ttgcagtaca cgccgtcgac
     5641 accgtcgagc gcgatatagc caagcgcgtt ctttatgatg tgcgctttga cggtgagcag
     5701 accgccggct tcgatgattg ccttcgcgcg ctcccactgc gacgtcgcct ggaagtttgt
     5761 cgcaaaatgc acgagattcg tcccgccgac gagcgacgga tagatcaacg acgccccctt
     5821 gatgccgctc atgtttgtcg tcgcggcagc gctgatctcg gtcttgatgt cgcgcgaaga
     5881 ggcgacgaaa tcgaacccga gctcgttcat tgccgtgagc aacgcggggg gcgcgttcca
     5941 cccgggggga cacatcccgc gcacgtacgg cagcttcgac gcctcgaaga tg
//
LOCUS       DASUFX010000650        10448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1076393, whole genome shotgun sequence.
ACCESSION   DASUFX010000650 DASUFX010000000
VERSION     DASUFX010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076393"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFV99_26380"
     CDS             complement(<1..1227)
                     /locus_tag="VFV99_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062940.1"
                     /translation="MARTFDTPIQHLDECVALVHQLVERQINAHWEAGILPRVKDQFS
                     GTFVSGGEVVALLGGAVPSPEAQQKLVDLDAAISAREERIEAKLEEARRQDQTLPFDV
                     LRRAFALTPTEQRALWILIALEVNAPLRQLMRYLVNEASRVYADVGLLQLLVYSTDAT
                     RELMLHELSPESRLFRYRLVEFLGSRRQQEDAPFLLRQVKVHPRVIELVHGIVRLDRE
                     VADIAELLHSLPPHDGLVLPDGIKHETVQLVRNALEASNGGTVSQAIVLSGSEGSGRK
                     SLVYGAARAAGCSVVRIASDRLPADPLELQRTVRALLREAALFHAMPMFERIDVLAGD
                     VELGRVDRSAVLDRELASFLGPVAGTCTRRDTRPLALSRGLVIVDVPMPTEAARAQLW
                     ERALGMDARGFDVDRAA"
     gene            1283..1981
                     /locus_tag="VFV99_26385"
     CDS             1283..1981
                     /locus_tag="VFV99_26385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062941.1"
                     /translation="MELVGLTSELRDCLLAGTGKPPLDVALELVGRAGAAGLAILGAM
                     SIDRRLAPHQRMTVLLAISVLLALTPETPPAEALDAVEDEETAATAIRSGYGAALTGV
                     LAAAPRGGSLDATRRLLGRRLVAAGERGPHVAQLLLDAGDRVRAAQLAADDLAQHLAT
                     HAEPTLETWARLRILLDWSSDDALLDAVLQQLATQHPALLARLEPLLVRIPDAEPAAV
                     QRVRSTLVWLLNSR"
     gene            complement(1968..2510)
                     /locus_tag="VFV99_26390"
     CDS             complement(1968..2510)
                     /locus_tag="VFV99_26390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062942.1"
                     /translation="MMQEARSISEARLFIEREPCPACGHRGFGHPSKLVENGERIAAV
                     ASGYCPSCGTERTFELLLGDERVPDGAWGSAARSTLIDAGEWLAIADDFGARVPAEIE
                     TLDRGARDVARADLAKGIAAVEEVLKFFPPGSDELPRDAFFTKRGLELYLAQPARYRR
                     DRLDVLLDAWRELAESFIEN"
     gene            complement(2507..3112)
                     /locus_tag="VFV99_26395"
     CDS             complement(2507..3112)
                     /locus_tag="VFV99_26395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062943.1"
                     /translation="MAAAAVVLVAACGSQNGAQKKPARDCDSYAKKVADLSDPAPGKR
                     DGLVAHSVERCEAGRIPDAELTCVAGAKNRAELIECTTGAPPMDSSPPPPAAKGTNKV
                     TLLTAVNGEGFHPLDLALNEDQRTWFEGLKKDIAACATDEVYDPPKQYVVVVTFSSEA
                     PAISLGGLPAPLSFCVKQVLQRRQPNSVGVGPAEFYIDVGR"
     gene            3280..4140
                     /locus_tag="VFV99_26400"
     CDS             3280..4140
                     /locus_tag="VFV99_26400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062944.1"
                     /translation="MATLGSAYRLDLTALFDRLSALAPELAKTAIVTVPRTFDRLAIS
                     TNITRGNDGIDRVRVLAGVCDPAQPARLATLFGAETKDLVVEVESMPHDPQAFALSVT
                     GYGRRSLASDLAHLGSFGVDVDSLGELASSLIGADRLIGLADRADTTGARAWTLHVAH
                     HNGDDAQHNATRAQVGTVAEKLGISAPQRNLVAGLHDALTNGRDSYSWLRVRPQMPTQ
                     LGVLWPTVRWEDVVNIATTLYPTSASGTRLGELAGAFDVDAASGVELELTTTDPPDMR
                     VLVTVAKGAA"
     gene            4143..>10448
                     /locus_tag="VFV99_26405"
     CDS             4143..>10448
                     /locus_tag="VFV99_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062945.1"
                     /translation="MTKEFSPDSSQAKKPDAKATPGVTPPAPTNNLDGEKAATFLRLM
                     GNITKDWKAVLGSLATDTETPPNPPTSKAFQADAYRLLARRDKTGETGLDAVRQLLAL
                     TQTRMPYAQDLERTESIVGADTARRTAIHECLQDLQAGGDIKYNTDRLVVTYMAQSQL
                     GRDWWQVPSQDVNEWLGPFAAYLTDTGTLWKHCMEDGIATAPSLAAKRASQLKPLLNL
                     LKLGMLTLPEAGKTATPAAPAAAAGPDDAQKKAWDEEAAKLKGEIEPHLPPDGATGED
                     AKLKNLDRKQRLDLILKITAASLEVKDRLSSDVVFMRRVALLGETTSHGGVKETQQSL
                     FRALDPVEALADQVSGTTVAKSGMLVEHGGVHAAGDKDKHFTAIRDFLASHGDKDPAK
                     AKAQEPIRLRVMTHTKIQAACRMFSEPQQQDLWKLATRGTLEPQLSDKLFPAVKAGNT
                     KEVAKVLLTMSADDRASMAGLKDDLLFRNLIASDKMREQVEIDGITVRPYDLCLLMWG
                     LRPGLSKDPGQTGPEEACDPNLKPLNFDDRVNLDQKLYKPTIKKLKDDLSAADSWFAS
                     GWRTDDDDVASHLDQFATQCASKDFLELIRRGGTPPGQELMTKYNAETGKSLHSLIKS
                     GCGDKARRAAERVLNISIGSESVGLVGGVIHRAGDGKENAQMKLEQALRETPTALGGR
                     SIQQVVHSAAADMRRELYEGLCWNGADLEDVYEIWDNLAKAVTPDLLKQVKEKTGETV
                     FTIELFQNAYQEIDGPLINALNKRLSDSDGNKAKARMGLTDAGAASRRKDAQGADTPV
                     STEAAARQDAEQKFGSQAKELFGALSALDFKARQEQFLSAKTKLIGFLTTQAPTAGAP
                     PAQLKITTEAVIGEKAADAKAGAGLGTVQTPDDYYRLKFGITPKNHAVQIVRSFKFAK
                     DDAESTDREQQLTPKEVAEWLGVDAGMIDSPQLPPVGSKVVIDDQNKNLVRQGFTEAT
                     ATDNANEMWKLLHDGGEVRLIEATLYGPYNDEEKRLIRMAFRQLSGGIDWQFYVQQAL
                     YQYEHAGQKSDYHHGMRVGAEGTMAGQGIAGSNKINVTADKGELDVLVALGTEGEVDI
                     KAELNNAISHKEWNQVLRIVDRADDGQCRAILADAKLMGRLESYLDAYSWDRVHRVLT
                     GQDGLGVRLESRSHGNKDEFFEGTLGFGGTDESGMKDDIKEYVHRLTNKFQAEEVKKA
                     KADNKVPNQDLILMTVARRVREACASLAADPEINAIIERELSGYDLSSTRGQLLSGTG
                     EDSNVAAVTADEHDAEKVLAEIRKMQKPERERRLKDPEYMARLSQLIHGKDLQQAISL
                     LQSTADEAQAAQEGTEAKSHDNLAEIVKLSAPTGSHDVDDLLAKLVELSPEEHARLLA
                     DPKLVAQVTAVFHNTSKEKREIAERVLGFRKEQADKLLGPGVTNPDGATNPQGRTVSP
                     EERQRLAFLQENAVARIQQGATVSWNQLLGQLVEVYKMDFHPKLIAPQPAGQPAGQPA
                     GQQAPAQTLDEPAKFDPNEATKITLEQQLRMGILDRIRENASVLERADSPLKKERVRL
                     AVLGLSDPSSFRVLEQWGAFDDDEAGVEDTLAKASDDHLIDWWTSIRLEKPNGDDSLA
                     TVYDRYRKAHDKVEAQRKLDPNAPKDMPADVRGLRDAFMNYVVDISGDFEDLILNFAG
                     GVFSDDSKTKKDKKRSKVKQRDNEDYQKWRGIIRSRIPTLPATKIAKKIHADDRPGDV
                     ELITNPNRKARTDFEFSEDEYTRRLGTEGSGARVAGGKRATVTDTLTRYGQELATAES
                     NKDGGVGIITKEEGEGLAKTHGDFELAMKEFREARAKIASIAGAIVGLVAGAIVTIIT
                     GGAGAGLAWMLVAGALSGAAGAAGTALTKEMIQGSEFDFSHEGLTSIAQGAITGMIMA
                     GTTRIAGNIMKSLTGPATMKAQATALQHVLNGTKPDLWEKVVTAAQGLGFATGEGVLS
                     EGLNIGIEAGIAPLDPSLWVHGWDEGVIRARHKIRDHISAAPQRLFNAGVMSALTHGV
                     GALRGKGAEGGTPVQPPHSDLEKKAVKGSLEWHVARMKDNVKAYMKVDEVMVMAFSSF
                     VSQKISQAVFDKPIDWNNPQEQIFIEFL"
BASE COUNT         1980 a         3521 c         3449 g         1498 t
ORIGIN      
        1 ggccgcgcga tcgacgtcga agccacgtgc gtccatcccg agcgcgcgtt cccatagttg
       61 cgcacgtgcg gcttcggtcg gcatcggtac gtcgacgatg acgagcccac gtgacagcgc
      121 gagcggccgc gtgtcacggc gcgtacacgt gccggcgacc gggccgagaa agctcgcgag
      181 ctcgcggtcg agcaccgcac tgcgatcgac gcggccgagt tcgacatcgc cggccagcac
      241 gtcgatccgt tcgaacatcg gcatcgcgtg gaacagcgcc gcctcgcgaa gcagcgcgcg
      301 caccgtgcgc tgcagctcga gcggatctgc cggtaggcga tcggatgcga tgcgcaccac
      361 gctgcagccc gccgcacgcg ccgcgccata gacgagcgac ttgcgaccgg atccttccga
      421 gccgctcagc acgatagctt gcgagacggt tccgccgttc gatgcttcga gtgcattgcg
      481 cacgagctgg accgtctcgt gcttgatgcc atcgggcagc acgagcccgt cgtgcggcgg
      541 caagctgtgc agtagctcgg cgatgtcggc aacctcgcga tcgagccgca cgatgccgtg
      601 caccagctcg atcacacgcg ggtgcacctt cacctgccgg agcagaaagg gcgcgtcctc
      661 ttgctggcgg cggctgccga gaaactcgac gagccgatag cgaaacaacc ggctctcggg
      721 cgacagctcg tgtagcatca gctcgcgcgt cgcatccgtc gagtagacga gcaactgcag
      781 caggccgacg tcggcgtaaa cgcgcgacgc ttcattgacg aggtagcgca tgagctgccg
      841 cagcggcgcg ttgacctcga gcgcgatcag gatccacaac gcgcgctgct cggtcggcgt
      901 cagcgcgaac gcgcggcgta gcacgtcgaa cggcagcgtc tgatcttgcc gccgcgcttc
      961 ctcgagcttc gcttcgatcc gttcctcgcg cgcgctgatc gcagcgtcga ggtcgacgag
     1021 cttctgttgc gcctcgggcg acggcaccgc gccaccgagc agcgcgacca cttcgccgcc
     1081 ggatacgaat gtgccggaga actgatcctt gacccgcggc aggatcccgg cctcccagtg
     1141 cgcgttgatc tggcgttcga cgagctgatg aaccagcgca acgcattcgt cgaggtgctg
     1201 gatcggtgta tcgaaggtgc gagccactcg cgccaagctt accgtatgga ccgagccgca
     1261 atcgtcttac aatggcggct cggtggagct tgttgggctg acctcggagc tgcgcgactg
     1321 tctgctcgcc ggaacgggaa aaccaccact cgacgtggcg ctcgagctcg tcggccgtgc
     1381 cggcgcagcc gggctcgcga tcctcggcgc gatgtcgatc gatcgccgac tcgctccgca
     1441 ccagcgcatg accgtgctgc tcgcgatctc ggtcctcctc gcgctgacgc cagagacgcc
     1501 gcccgccgag gcgctcgacg ccgtcgagga cgaggagact gccgcgaccg cgatccgttc
     1561 cggatacggc gcagcgctga ccggcgtgct cgcggcggcc ccgcgtggtg gctcgctcga
     1621 tgcgacgcgg cggttactcg gccgcaggct cgtcgcggct ggcgaacgcg gaccgcatgt
     1681 cgcgcagctg ttgctcgacg ccggtgaccg cgtgcgcgcg gcgcaacttg cagccgacga
     1741 tctcgcgcag cacctcgcga cgcacgccga gccgacgctc gagacgtggg cgcggctgcg
     1801 gatcttgctc gactggagca gcgacgacgc gcttctcgac gccgtgttgc aacagctcgc
     1861 gacgcagcat cccgctctgc tcgcccggct cgagccgttg ctcgtgcgca ttcccgatgc
     1921 cgagccggct gcggtgcagc gggtgcgttc gacgctcgtg tggctactca attctcgatg
     1981 aacgactcgg cgagctcgcg ccacgcgtcg agcaacacgt cgaggcgatc gcggcggtaa
     2041 cgcgcgggct gcgcgaggta cagctcgagc ccgcgcttgg tgaagaacgc atcacgcggc
     2101 agctcgtcgc ttcctggcgg gaagaacttc agcacttcct cgacggcggc gatccccttc
     2161 gcgagatccg cgcgtgcgac atcgcgggcc ccgcgatcga gcgtttcgat ctcggcggga
     2221 acgcgcgcgc cgaagtcatc ggcgatcgcg agccactcgc cggcgtcgat tagcgtcgag
     2281 cgagccgcac tgccccacgc gccatcgggc acgcgctcgt cgccgagcag cagctcgaac
     2341 gtgcgctcgg tgccgcagct cgggcagtat ccgctcgcga ccgcagcgat ccgctcgccg
     2401 ttctcgacga gcttgctcgg atgaccgaaa ccgcgatgcc cgcacgctgg gcatggctcg
     2461 cgctcgatga agaggcgtgc ttcgctgatg ctgcgcgctt cctgcatcac cgaccgacgt
     2521 cgatgtagaa ctcggcggga ccgacgccga ccgaattcgg ctggcggcgc tgcagcacct
     2581 gtttcacgca gaacgacagc ggtgctggca gtccgccgag cgagatcgcg ggagcttcgg
     2641 agctgaacgt cacgacgacg acgtactgct tcggcggatc gtagacctca tcggtcgcgc
     2701 acgcggcaat gtctttcttg agaccctcga accacgtccg ctgatcttcg ttgagcgcga
     2761 gatcgagcgg atgaaatccc tccccgttta ccgcggtgag cagcgtgacc ttgttcgtgc
     2821 ccttggcggc cggcggtggc ggtgatgaat ccatgggtgg cgctcctgtc gtgcactcga
     2881 tcagctcggc gcgattctta gcgccggcga cacaggtcag ctccgcatcg gggatccgcc
     2941 ccgcctcgca acgctcgacg ctgtgcgcga ccaacccgtc ccgcttgccg ggggccggat
     3001 cgctcagatc tgcgaccttt ttcgcgtagc tatcgcagtc acgggccggc ttcttctgcg
     3061 cgccgttctg cgagccgcac gcagcgacca agaccaccgc cgccgcggcc atccgcaaga
     3121 ccatcgagcg ctagcctaca cgaggggatt cagttgttaa accggtacgg cgccagagaa
     3181 aactggcgca ccgatcgagg gaaggctggc gtgcgcttcg cgtaggatgg tggcgtgcac
     3241 gggctcgagc tcgataactc catctctgtc ttcggtcggg tggcgaccct cggtagtgcg
     3301 tatcgtctcg atctcacagc gcttttcgac cgactttcgg cgctcgcgcc cgagctcgcg
     3361 aagaccgcga tcgtaaccgt cccgcgcacg ttcgatcgcc tcgcgatcag cacgaacatc
     3421 acgcgcggta acgacggcat cgatcgcgtg cgcgtgctgg caggcgtctg cgatcccgca
     3481 caaccggcgc gcctcgccac gctgttcggc gccgagacga aagatctcgt cgtcgaggtc
     3541 gaatcgatgc cgcacgatcc gcaggcgttc gcgctcagcg tcaccggcta cggcaggcgc
     3601 agcctcgcga gcgacctcgc gcatctcggc tcgttcggcg tcgacgtcga tagcctcggc
     3661 gagctcgcca gctcgctaat cggcgccgat cggttgatcg gcctcgccga tcgcgccgac
     3721 acgacgggcg cacgcgcgtg gacgctccac gtcgctcacc acaacggcga cgatgcgcag
     3781 cacaacgcaa cgcgcgcgca ggtcggaacg gtcgcggaga agctcggcat cagcgcgccg
     3841 caacgcaacc tcgtcgccgg actgcacgac gcgctcacga acggccgcga cagctacagc
     3901 tggttgcgcg tccgcccaca gatgccgacg cagctcggtg tgctgtggcc aacggttcgc
     3961 tgggaggacg tggtgaacat cgcgacgacg ctgtatccga cgagtgcgag cggtacgcgg
     4021 ctcggcgagc tcgccggcgc attcgacgtg gacgcagcgt ccggcgtcga gctcgagctc
     4081 acgacgaccg atccgcccga catgcgcgtg ctcgtgactg tggcgaaagg cgctgcgtag
     4141 ctatgaccaa agagtttagc cccgactctt cacaggcgaa aaaacctgac gccaaagcga
     4201 ccccgggtgt cacgccacct gcgccgacga acaacctcga cggagagaag gccgccacgt
     4261 tcctccgcct gatgggcaac atcaccaagg actggaaggc cgtcctcggc agcctcgcca
     4321 ccgataccga aacgccgccg aatcctccga cgtcgaaagc gttccaggcc gacgcgtacc
     4381 gcctcctcgc tcgtcgcgac aagaccggcg agaccggact cgacgcggtc aggcagctgc
     4441 tcgcgctcac gcagacacgc atgccgtatg cccaagacct cgagcgcacc gagtcgatcg
     4501 tcggcgccga caccgcgcgc cgcaccgcga tccacgagtg cctgcaagac ctccaggcag
     4561 gcggcgacat caagtacaac accgaccgcc tcgtcgtcac gtacatggcc caatcgcagc
     4621 tgggccgcga ctggtggcaa gtgccgtcgc aagacgtcaa cgagtggctc ggtccgttcg
     4681 cggcatacct gaccgatacc ggcacgctgt ggaagcactg catggaggac ggtatcgcta
     4741 cggcgccgtc gctcgcggcg aagcgtgcat cgcagctcaa gccgctgctc aacttgctca
     4801 agctcggcat gctcacgctg cccgaggccg gcaagaccgc aacgccggcg gcgcctgccg
     4861 cagcggccgg ccccgacgac gcgcagaaga aagcgtggga tgaggaagcc gcgaagctca
     4921 agggcgagat cgagccgcac cttccgcccg atggcgcgac cggcgaggac gcgaagctca
     4981 agaacctcga tcgcaagcag cgcttggatc tgatcctgaa aatcaccgcg gcgtcgctcg
     5041 aggtgaaaga tcggctgagc tcggacgtgg tgttcatgcg ccgcgtcgcg ctgctcggcg
     5101 agacgacgag ccacggcggc gtgaaggaaa cgcagcaatc gctgttccgc gcgctcgatc
     5161 cggtcgaggc actcgccgat caagtcagcg gaacgacggt tgcgaagtcc ggcatgctcg
     5221 tcgagcacgg cggcgtgcat gccgcgggcg acaaggacaa gcacttcacc gcgatccgcg
     5281 acttcctcgc gagccatggt gacaaagacc cggcgaaggc caaggcgcaa gagccgatcc
     5341 gtctgcgcgt gatgacgcac acgaagatcc aggcggcgtg ccgcatgttc tccgagccgc
     5401 agcaacagga tctctggaag ctcgcgacgc gcggcacgct cgagcctcag ctgtccgaca
     5461 agctgttccc ggcggtgaag gccggcaaca cgaaggaggt cgcgaaggtc ctgctgacga
     5521 tgagcgccga cgatcgcgcc tcgatggccg gcctcaagga cgacctcttg ttccgcaacc
     5581 tgatcgcgag cgacaagatg cgcgagcagg tcgagatcga cggcatcacg gtgcgtccgt
     5641 acgacctgtg cttgcttatg tggggactgc gccccggcct cagcaaggat ccgggtcaga
     5701 ccggtcccga agaggcctgc gatccgaacc tcaagccgct caacttcgac gaccgcgtca
     5761 acctcgacca gaagctctac aagccgacga tcaagaagct caaggacgac ctctcagccg
     5821 cggattcgtg gttcgcgtcg ggctggcgca ccgacgacga tgacgtcgcg agccacctcg
     5881 atcagttcgc gacccagtgc gcgtcgaaag acttcctcga gctgatccgg cggggcggca
     5941 cgccgcccgg ccaggagctg atgacgaagt acaacgccga gaccggcaag tcgctccact
     6001 cactgatcaa gagcggctgt ggtgacaagg cgcgccgcgc ggctgagcgc gttctcaaca
     6061 tttcgatcgg tagcgagagc gtcggcctcg tcggtggcgt cattcatcgc gccggtgacg
     6121 gaaaagaaaa tgcgcagatg aagctcgagc aggcgctgcg cgaaacgccg accgcgctgg
     6181 gcggccgatc gatccagcag gtcgtgcact cggccgcggc ggacatgcgc cgcgagctct
     6241 acgaaggtct gtgctggaac ggcgccgacc tcgaggacgt ctacgagatc tgggacaacc
     6301 tcgcgaaggc ggtcacgccg gacctgctga agcaggtcaa ggagaagacc ggcgagacgg
     6361 tcttcacgat cgagctgttc cagaacgcgt accaggagat cgatggcccg ctgatcaacg
     6421 cgctcaacaa gcggctcagc gactccgacg gcaacaaggc gaaggcgcgc atgggtctga
     6481 ccgacgccgg tgccgcgagc cgccgcaaag acgcgcaggg tgcagacacg ccggtcagca
     6541 ccgaagcagc cgcgcgccag gacgccgagc agaagttcgg cagccaggcg aaggagctgt
     6601 tcggcgcgct gtcggcgctc gacttcaagg cgcgccagga acagttcctc tccgctaaga
     6661 cgaagctgat cggctttctc acgacgcagg ccccgacggc tggcgcgccg cctgcccagc
     6721 tcaagatcac gaccgaggcg gtgatcggcg agaaggcagc cgatgcgaaa gcgggcgccg
     6781 gtctcggcac cgtgcagacg cccgacgact actaccggct gaagttcggc atcacgccga
     6841 agaaccatgc ggtgcagatc gtgcgctcgt tcaagttcgc caaagacgac gccgagtcga
     6901 ccgatcgcga acaacagctc acgcccaagg aagtcgccga gtggctcggc gtcgacgcgg
     6961 gcatgatcga cagcccgcag ctgccgcccg tcggctcgaa ggttgtcatc gacgaccaga
     7021 acaagaacct cgtccgccag ggcttcaccg aggccaccgc gaccgacaac gcgaacgaga
     7081 tgtggaagct gctccacgac ggcggcgagg ttcggctcat cgaagcgacg ctgtacggac
     7141 cgtacaacga cgaagagaag cggctcatcc gcatggcgtt ccgccagctg tcgggcggca
     7201 tcgactggca gttctacgtc cagcaggcgc tctaccagta cgagcacgcc ggccagaaga
     7261 gcgactacca ccacggcatg cgcgtcggcg ccgaaggcac gatggccggt caaggcatcg
     7321 ccggttcgaa caagatcaac gtaaccgcgg acaagggcga gctcgacgtg ctggtcgcgc
     7381 tgggcaccga gggcgaagtc gacatcaagg ccgagctgaa caacgcgatc tcgcacaagg
     7441 agtggaacca ggtcctgcgc atcgtcgatc gcgcggacga cgggcagtgc cgcgcgatcc
     7501 tcgccgacgc gaagctgatg ggacggctcg agtcgtacct cgacgcgtac tcgtgggatc
     7561 gcgtgcaccg cgtgctcacc ggccaggacg gcctcggcgt gcgcctcgag tcgcgctcgc
     7621 acggcaacaa ggacgagttc ttcgagggca cgctcggatt cggcggcacc gacgagtccg
     7681 gcatgaagga cgacatcaag gagtacgtcc accgcctgac gaacaagttc caggccgaag
     7741 aggtaaagaa ggcaaaggcc gacaacaagg tcccgaacca ggacctgatc ctgatgacgg
     7801 tcgcgcgccg cgtgcgcgag gcatgcgcgt cgctcgccgc cgatccggag atcaacgcga
     7861 tcatcgagcg cgagctgtcg ggctacgatc tgtcgtcgac gcgcggtcag ctgctgagcg
     7921 gcaccggcga ggactcgaac gtcgccgcgg tgaccgctga cgagcacgat gcggagaagg
     7981 tactcgccga gatccgcaag atgcagaagc cggagcgcga gcgtcgcctc aaggaccccg
     8041 agtacatggc gcggctcagc cagctgattc acggcaagga tctccagcag gcgatttcgc
     8101 tgctgcagtc gaccgccgac gaggcgcaag ccgcacagga aggtaccgag gcgaagtcgc
     8161 acgacaacct tgccgagatc gtgaagctgt cggcgccgac cggctcgcac gatgtcgacg
     8221 acctgctcgc gaagctcgtc gagctcagcc ccgaagagca cgcccgcctg ctcgccgacc
     8281 cgaagctggt cgcgcaagtc accgcggtgt tccacaacac gagcaaggag aagcgcgaga
     8341 tcgccgagcg cgtgctcggc ttccgcaagg aacaggcgga caagctgctc ggcccgggtg
     8401 tcacgaaccc cgacggcgcc acgaatccgc agggccgtac ggtcagcccc gaggagcgcc
     8461 agcgcctcgc gttcctccag gagaacgcgg tcgcgcgcat ccagcaaggc gcgacggtca
     8521 gctggaacca gctgctcggc cagctcgtgg aggtctacaa gatggacttc cacccgaagc
     8581 tgatcgcgcc gcagccggcg ggtcagcccg cgggtcagcc ggcgggtcag caagcgccgg
     8641 cgcagacgct cgacgagccg gcgaagttcg atccgaacga ggccacgaag atcacgctcg
     8701 agcagcaact gcgcatgggc atcctcgatc gcattcgcga gaacgcctcc gtgctagagc
     8761 gtgccgacag tccgctcaag aaggagcgcg tgcggctcgc ggtgctcggc ctctccgatc
     8821 cgtcgtcgtt ccgcgtgctc gagcagtggg gcgcgttcga cgacgacgaa gcaggcgtcg
     8881 aggacacgct ggcgaaggca tccgacgatc acctgatcga ttggtggacc tcgatccggc
     8941 tcgagaagcc gaacggcgat gattcgctcg cgacggtgta cgaccgctat cgcaaagcgc
     9001 acgacaaggt cgaagcgcaa cgcaaactcg atccgaacgc gccgaaggac atgccggcgg
     9061 acgtcagagg tctgcgcgac gcgttcatga actacgtcgt cgacatctcc ggcgacttcg
     9121 aggacctcat cctcaacttc gccggcggcg tgttcagcga cgactcgaag acgaagaagg
     9181 acaagaagcg cagcaaggtc aagcagcgcg acaacgagga ctaccagaaa tggcgcggca
     9241 tcatccgcag ccgcattccg acgctgcccg cgacgaagat cgcgaagaag atccacgccg
     9301 acgatcggcc gggcgacgtc gagctgatca cgaatccgaa ccgcaaggcg cgcaccgact
     9361 tcgagttcag cgaggacgag tacacccgcc gcctcggtac cgagggctcc ggcgcacgcg
     9421 tcgccggcgg caagcgcgcg accgtcaccg acacgctgac ccgttatggt caggagctcg
     9481 cgaccgccga gagcaacaaa gacggcggcg tcggcatcat cacgaaggaa gaaggcgaag
     9541 gcctcgcgaa gacgcacggc gacttcgagc tcgcgatgaa ggagttccgc gaggcacgcg
     9601 caaagatcgc gagcatcgcg ggcgcgatcg tcggcctggt cgcgggtgcg atcgtcacga
     9661 tcatcaccgg cggcgccggt gcaggcctcg cgtggatgct cgtcgccggc gcgctatccg
     9721 gtgcggccgg tgcggccggt accgcactca cgaaggagat gatccagggc tcggagttcg
     9781 acttcagtca cgagggtctg accagcatcg cgcagggcgc gatcaccggc atgatcatgg
     9841 cgggcacgac ccgcatcgcc ggcaacatca tgaagtcgct gaccgggccc gcgacgatga
     9901 aggcgcaggc cacggctctc cagcacgtcc tcaacgggac gaagcccgac ctctgggaga
     9961 aggttgtcac cgccgcgcag ggtctcggct tcgcgaccgg cgaaggcgtg ctctccgaag
    10021 gtctcaacat cggcatcgaa gccggcatcg caccgctcga tccatcgctg tgggtacacg
    10081 gctgggacga aggcgtgatc cgcgcgcgcc acaagatccg cgatcacatc agcgccgcgc
    10141 cgcagcgctt gttcaacgcg ggcgtgatga gcgcgctcac gcacggcgtc ggcgcgttgc
    10201 gcggcaaggg tgccgagggc ggtacgccgg tgcagccgcc gcacagtgac ctcgagaaga
    10261 aagccgtgaa gggcagcctc gagtggcacg tcgcgcgcat gaaggacaac gtgaaggcct
    10321 acatgaaggt cgacgaggtc atggtcatgg ccttctcgag cttcgtgtcg cagaagattt
    10381 cccaggcggt gttcgacaag ccgatcgact ggaacaatcc gcaggaacag atcttcatcg
    10441 agttcctg
//
LOCUS       DASUFX010000651         6761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_938388,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000651 DASUFX010000000
VERSION     DASUFX010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938388"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="VFV99_26410"
     CDS             <1..1725
                     /locus_tag="VFV99_26410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5062946.1"
                     /translation="RPRDAQKSVGALVDQALSLVDDLSADELDKARIAAEAGFVRQLE
                     TAQGRARTLGWNASLTGDPRFAHVYLDRIRAVRRHDISATVRRYFRPENATVAAILPK
                     QSARVHATFARSAEKKVRSALRVAAAPPTVAEKRVALGNGMTIVVRRDPSVPIVAMRA
                     VWRGGQRVEDDDHAGASTLLARLITRGCGKLDATALADRVDRLGGSLGGVSGRNSFGV
                     AAEWLAKSWHSGFDLLADCILQPTLAAAELAREKRALLDDQIAQGDNPTQVAFRLFSE
                     TLYGAHPYARDVLGTQAAIDALDRAALAAFYRQRYPVSQLTLSIVGDVDIDDVVARVT
                     KRFGASKSVKAPLPVISPPRFDGRDDTEREVYRFLDRAQAHLVIGYPGATVDAKDRFA
                     LEVLVAVLGGQSGRLFEELRDKQGLVYRVSAHSVEGVDPGFVAVYLSCAPDKLDAAVT
                     SVRKELDRVRTSGITAEELERAKSYLIGSHQIAMQRRSAVANAIAYHEAYGLGWRTWA
                     AYDDAIRAVTVDDVAAAATAYLRSDRAITATVRPPVRTPGAAKRSKLPVDKKPASAAK
                     SRRPRPSS"
     gene            1736..2404
                     /locus_tag="VFV99_26415"
     CDS             1736..2404
                     /locus_tag="VFV99_26415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5062947.1"
                     /translation="MKRGQLTTTGHGDLEASFVLIFPLLLAYEIGVLFAGRVNGADVV
                     TRAVYAIAPSRAAYLLIYACVAVLFLLWIRREGRYRTLRLEIAAPVILEAAIYALTLG
                     ALITVVLDRLLGLSLDGNSVVAALGAGVHEELVFRLGLFAGFVALLEGLERRFAITLA
                     LVTSSLLFAAAHHLGAHGEPFTMHAFAFRALAGAAFGAIFWFRSFAHAVYAHVLYDLV
                     VAAS"
     gene            complement(2418..3341)
                     /locus_tag="VFV99_26420"
     CDS             complement(2418..3341)
                     /locus_tag="VFV99_26420"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX5062948.1"
                     /translation="MADKLAYTAGHFELLIDGAKQTTYLKSLDGGFWGHSAVDEAIGT
                     ENKRIKHSAVADVEPISVEIGMAGSTSVMKWIQDSWDKNYQQRSGQINHANFNMETVF
                     EHEFSDALITETTFPALDGASKDAAYLKVKFQPKAVATRKVFKSQKLSPIGGLKQKQW
                     TCSAFRLNIDGIDDAIYTNKIESFTIKQTVKKLFTGMHRDIELCPTKIEFPNISATIS
                     LEKADGILAWYDKYVVKGKKDPAAQKGGSLEFLTPNRDKTLFRVTFAAMGLLKAAIVQ
                     STANSDQIKRVKFDMYVGEMKLDGPGALGLE"
     gene            3490..5130
                     /locus_tag="VFV99_26425"
     CDS             3490..5130
                     /locus_tag="VFV99_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062949.1"
                     /translation="MRISALILLVVLGTVSVAHAEKVKTNQSTKLFAGPGEQKKVLLK
                     VKSGQNMTLLSQDGRWLKVRVQGRTGYVPRTKVDMADDGEIVRNTRRRPFVDGRSRRR
                     GFGGEAGPDDRVGADALGEGQEDSGSSSDEGDDEEEDTPKKPAKKTAAKKVVEDEEED
                     EAPKKPVAKKAAAKKVVEDDEEDEPKKSSGDEEDEEDSPKEGSDEEDEGDEVVEDEAD
                     KRPTARVSKRVSVYEEADGDSEELFVARPTDTLFPLESKGKWTQVENAEGDLGWVETD
                     YLDMDGGGGGMKKRQIDVTARMGLMFIQQGMRTQGSTNLKVPDNYNINTSSVTLSLGG
                     AYTRPFKKKYILGGEATYDYSNTLFGGVFYDPDGKAGPMPGVNTKLTLHNINIRGMAG
                     IDLKKKSGMAFFGRLGYRYQSYLVAGYSDPAKNPGKIPQEIMKAPTLGVAMIMPTLTP
                     KIGLRVSLDTILFGASLVQTKGLEDGTSPTLKAVTFGTAVTYRWKKNMDLQATYDLRY
                     AGIDFGPPGTQSMRGHMGTNVSRTDFLHMVTFGIAKPF"
     gene            complement(5102..6022)
                     /locus_tag="VFV99_26430"
     CDS             complement(5102..6022)
                     /locus_tag="VFV99_26430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX5062950.1"
                     /translation="MSAIEVPTWAWALLGILLLVFITIDLLAHRGDHAESRKSALIWT
                     CFWVAIALGFNLFVYFQFGRTAGEQFLAAYLLEKSLSVDNLFLFLVIFGELRIPRTEQ
                     RRVLTFGILGALITRCVFIVLGAAAIARWHEVTYVFGALLLITSLKLLRTPDETGEPP
                     KALLWLERHLPWTRKLHGHKFLAREDGRLVATPLLIALVAIEVTDVIFAVDSIPAAFA
                     VSNDTFILYSSNVFAVLGLRALYIVLASALHGLRYLRFGLSAVLAFAGIKMLVVSWIK
                     VPPLVSVCVIASFIGIAIIASLRTASRSRT"
     gene            complement(6098..6361)
                     /locus_tag="VFV99_26435"
     CDS             complement(6098..6361)
                     /locus_tag="VFV99_26435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062951.1"
                     /translation="MVVRILAALVTLGLLVSAADARELSAPPTAAALVDAVDDTEDVA
                     LALPPTLTPEEFHVVNDFALPEAPAGHQPCVYVFRPPRAYAFN"
     gene            complement(6400..>6761)
                     /locus_tag="VFV99_26440"
     CDS             complement(6400..>6761)
                     /locus_tag="VFV99_26440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5062952.1"
                     /translation="IMIGVDEAPRERYAGGWARLRVRDNGVGISEEDRQRIFDPFAHP
                     KPVKHHTSAWPDSAGLGLYIARGLIELHGGLITVDSQPDVFTEFTVLLPFKQPRTRPA
                     SSDPPTTTQTPPAQTSS"
BASE COUNT         1217 a         2155 c         2252 g         1137 t
ORIGIN      
        1 cggccgcgcg acgcacagaa gagcgtaggc gcgctcgtcg accaggcact gtcgctcgtc
       61 gacgatctgt cggccgacga gctcgacaaa gcgcgtatcg ccgccgaggc cggcttcgtg
      121 cgtcagctcg agacggcgca gggcagggca cgcacgctcg gctggaacgc gagcctgacc
      181 ggtgatccgc ggttcgcgca cgtgtatctc gatcgcatcc gcgccgtgcg ccgccacgat
      241 atctcggcga ccgtgcgccg ctacttccgc cccgagaacg cgaccgtcgc ggcgattctg
      301 ccgaagcaat cggcgcgcgt gcacgcgacg ttcgcgcgca gtgccgagaa gaaggtccgc
      361 tccgcgttgc gcgtcgcagc ggcaccgccg accgtcgccg agaagcgcgt cgcgctgggc
      421 aatggcatga cgatcgtcgt gcgccgcgat ccgtcggtcc cgatcgtcgc gatgcgtgcg
      481 gtgtggcgcg gcggccagcg cgtcgaagac gacgatcacg ccggtgcgtc gacgttgctc
      541 gcgcgcttga tcacgcgcgg ctgcggcaag ctcgatgcga cggcgctcgc cgaccgcgtc
      601 gatcgtctcg gcggctcgct cggtggcgta tccggccgca acagcttcgg cgttgccgcg
      661 gagtggctcg cgaagtcgtg gcattcgggg ttcgatttgc tcgccgattg catcctgcag
      721 ccgactctcg cggcagccga gctcgcccgc gagaagcgcg cgttgctcga cgatcagatc
      781 gcgcagggcg acaacccgac gcaggtcgcg ttccggctgt tcagcgagac gctgtacggc
      841 gcgcacccgt acgcgcgcga cgtgctcggc acgcaagccg cgatcgacgc gctcgatcgc
      901 gccgcactcg ccgcgttcta tcggcagcgt tatccggtct cgcagctcac gctatcgatc
      961 gtcggcgacg tcgacatcga cgatgtcgtc gcgcgcgtca cgaagcgctt cggcgcgagc
     1021 aagtcggtca aagcgccgct gccggtgatc tcgccgccgc gcttcgacgg ccgcgacgac
     1081 accgagcgcg aggtatatcg cttcctcgat cgcgcgcagg cgcacctcgt gatcgggtat
     1141 ccgggcgcga ccgtcgacgc caaggatcgg ttcgcgctcg aagtgctggt tgccgtgctc
     1201 ggcggccaga gcggcaggct gttcgaagag ctgcgcgaca agcaagggct cgtctatcgc
     1261 gtgtcggcgc actcggtcga aggcgtcgac cccggcttcg tcgccgtgta tctgtcgtgc
     1321 gcgccggaca agctcgacgc ggcggtcacc agcgtgcgca aagagctcga tcgcgtccgc
     1381 acgagcggca tcacggccga ggagctcgag cgcgcgaaga gctacctgat cggtagccac
     1441 cagatcgcga tgcagcgccg ctcggcggtc gcgaacgcga tcgcgtatca cgaggcgtac
     1501 ggcctcggtt ggcgcacctg ggctgcctac gacgacgcga tccgcgcggt aactgtcgac
     1561 gatgtcgccg ccgctgcaac ggcgtatctg cgcagcgacc gcgcgatcac cgcgacggtg
     1621 cggccgccgg tccggacgcc gggcgcggcg aagcggtcga agcttcccgt cgacaagaag
     1681 ccggcgtccg ctgcaaagtc gcgccgtccg cgcccatcgt cgtagagttt ggcgcgtgaa
     1741 gcgcgggcag ctcacgacga cgggccacgg cgacctcgag gccagcttcg tcctgatctt
     1801 tccgctgctg cttgcctacg agatcggcgt gctgttcgcc ggccgcgtca acggcgccga
     1861 cgtcgtcacc cgtgccgtct atgcaatcgc gccgtcgcgc gcggcgtatc tgttgatcta
     1921 cgcgtgcgtc gccgtgttgt tcctgctgtg gatccggcgc gagggtcgct accgcacgct
     1981 gcgtctcgag atcgcggcgc cggtgattct cgaagctgcg atctatgcgc tcacgctcgg
     2041 cgcgctgatc acggtcgtgc tcgatcgcct gctcggcctc tcgctcgacg gcaactcggt
     2101 cgtcgccgcg ctcggtgccg gcgtgcacga agagctcgta ttccggctcg gcctgttcgc
     2161 aggcttcgtc gcgctgctcg aaggtctcga acgccggttc gcgatcacgc tcgcgctcgt
     2221 cacctcgtcg ctgctattcg cagccgcaca tcacctcggc gcccacggcg agccgttcac
     2281 gatgcacgcg ttcgcgtttc gcgcgctcgc aggcgcagca ttcggcgcga tcttctggtt
     2341 ccgatcgttc gcgcacgcgg tctacgcgca cgtgctctac gatctcgtgg tcgcggcgtc
     2401 gtagcccgga aggtcgatca ctcgaggccg agcgcgcccg ggccgtcgag cttcatctcg
     2461 ccgacgtaca tgtcgaactt gacgcgcttg atctgatccg agttcgccgt gctctgcacg
     2521 atcgccgcct tgagcaggcc catggccgcg aacgtgacgc ggaacagcgt cttgtcgcgg
     2581 ttcggcgtca ggaattcgag cgagccaccc ttctgcgcgg ccgggtcctt cttacccttc
     2641 acgacgtact tgtcgtacca agcaagaatg ccgtcggcct tctcgagcga gatcgtcgcg
     2701 ctgatgttcg ggaactcgat cttcgtcggg cacagctcga tgtcgcgatg catgccggtg
     2761 aacagtttct tcacggtctg cttgatcgtg aacgactcga tcttgttcgt gtagatcgcg
     2821 tcgtcgatgc cgtcgatgtt caaacggaac gccgagcagg tccattgctt ctgcttgagg
     2881 ccgccgatcg ggctgagctt ctggctcttg aagaccttcc tggtggcgac cgccttcggc
     2941 tggaatttca ccttgaggta cgccgcatcc ttcgacgcac cgtcgagcgc gggaaacgtg
     3001 gtctcggtga tcagcgcgtc ggagaactcg tgctcgaaca cggtctccat gttgaagttc
     3061 gcgtgattga tctggccgct acgctgctgg tagttcttgt cccacgagtc ctggatccac
     3121 ttcatcacgc tcgtcgagcc ggccatgccg atctcgacgg agatgggctc gacgtcggcg
     3181 accgccgagt gcttgatgcg cttgttctcg gtgccgatgg cctcgtcgac cgcgctgtga
     3241 ccccagaagc cgccatccag cgacttcagg taggtcgtct gcttggcacc atcgatcaag
     3301 agctcgaaat gcccagcggt atacgcgagt ttgtcagcca ttacgtgagc ctcagcttaa
     3361 cgcgaacctt ttacgattac tggcgcctgg atccccccga cggaactggt gaccccagca
     3421 actggctacg aaaacggcgt aatttcgcgt cattggatcc ggcatgcggc ttgtagaata
     3481 atgaggccca tgcggatcag tgcactgatc ctgctcgtcg tcctcggcac cgtcagtgtc
     3541 gcgcatgccg agaaggtgaa gacgaaccag tcgacgaagc tgttcgccgg tcccggtgag
     3601 cagaagaagg tcctactcaa ggtgaagtcg ggccagaaca tgaccctcct ctcgcaggat
     3661 ggtcgctggc tcaaggtccg cgtccagggt cggaccggct acgtgccgcg gacgaaggtc
     3721 gacatggccg atgatggcga gatcgtgcgc aacacgcgcc gtcgtccgtt cgtcgatggt
     3781 cgtagccgtc gccgcggttt cggcggtgaa gcaggccccg acgatcgcgt cggcgcagat
     3841 gcgctcggcg aaggccaaga ggacagcggc tcgagcagcg acgagggcga cgacgaggaa
     3901 gaagacacgc cgaagaaacc ggcgaagaag accgctgcga agaaggtcgt cgaggacgag
     3961 gaggaagacg aagcgcccaa gaaacccgtc gcaaaaaagg ccgcggcgaa gaaggtcgtc
     4021 gaggacgacg aagaagacga gccgaagaag tcgagcggcg acgaagagga cgaagaggac
     4081 tcgccgaagg agggcagcga cgaagaagac gagggcgacg aagtcgtcga ggatgaggcc
     4141 gacaagcgcc cgaccgcgcg cgtctcgaag cgcgtctcgg tctacgaaga agccgacggc
     4201 gattccgagg agctgtttgt cgcgcggccg accgacacgc tgtttccgct cgagagcaag
     4261 ggtaagtgga cgcaggtcga gaacgccgag ggcgatctcg gctgggtcga gaccgactac
     4321 ctcgacatgg atggcggtgg cggcggcatg aagaagcggc agatcgatgt caccgcgcgc
     4381 atgggcctga tgttcatcca gcagggcatg cgtacgcagg gcagtacgaa cttgaaggtg
     4441 cccgacaact acaacatcaa cacgtcgtcg gtgacgctat cgctcggcgg cgcgtacacg
     4501 cggccgttca agaagaagta catcctcggc ggcgaagcga cgtacgacta ctcgaacacg
     4561 ctgttcggcg gcgtgttcta cgatcccgac ggcaaggccg gcccgatgcc tggcgtcaac
     4621 acgaagctga cgctgcacaa catcaatatc cgcggcatgg ccggcatcga tctcaagaag
     4681 aagagcggca tggcgttctt cggtcgactc ggctaccgct accagagcta tctcgtcgcc
     4741 ggctactcgg atcccgcgaa gaacccgggc aagattccgc aagagatcat gaaggcgccg
     4801 acgctcggcg tcgcgatgat catgccgacg ctcaccccga agatcgggct gcgcgtctcg
     4861 ctcgacacga tcctgttcgg cgcgagcctc gtgcagacga agggcctcga ggacggcacc
     4921 tcgccgacgc tcaaggcggt cacgttcggc accgccgtca cgtaccggtg gaaaaagaac
     4981 atggacctgc aggcgacgta cgatctccgt tacgccggga tcgacttcgg gccaccagga
     5041 acgcagtcga tgcgcggtca catgggtacg aacgtgtcgc gcaccgactt cctccacatg
     5101 gtcacgttcg ggatcgcgaa gccgttctga ggctcgcgat gatcgcgatc ccgatgaagg
     5161 acgcgatcac gcagacactc acgagcggcg gcaccttgat ccagctcacg accagcatct
     5221 tgatgccggc gaacgcgagc accgcggaca acccgaaccg caggtaacgc aggccgtgga
     5281 gtgcgctcgc gagcacgatg tacagcgcgc gcaacccgag cactgcgaac acgttcgagc
     5341 tgtacagaat gaacgtgtcg ttggagaccg cgaatgccgc cgggatcgag tcgaccgcga
     5401 agatcacgtc cgtgacttcg atcgcgacga gcgcgatcaa cagcggcgtg gcgacgagcc
     5461 tgccgtcttc gcgcgcgagg aacttgtgcc cgtgcagctt gcgggtccac ggcaagtgac
     5521 gctcgagcca gagcagcgcc ttcggcggct cgccggtctc gtccggcgtg cgcagtagct
     5581 tcagcgacgt gatgagaagc agcgcgccga acacgtacgt gacctcgtgc cagcgtgcga
     5641 tcgccgcggc gccgagcacg atgaacacgc accgcgtgat caacgcgccg aggatgccga
     5701 acgtcaggac gcgtcgttgt tcggtgcgcg ggatgcgcag ctcgccgaag atgacaagga
     5761 acaaaaatag gttgtcgacc gacaggctct tctcgagcag gtacgccgcg aggaactgct
     5821 cacccgccgt tcttccgaac tggaaataga cgaacaggtt gaagccgagc gcgatcgcga
     5881 cccagaagca ggtccagatc agcgcgctct tgcgcgattc ggcatgatcg ccgcgatgcg
     5941 cgagcagatc gatcgtgatg aagacgagca gcagaatgcc cagcagggcc catgcccagg
     6001 tgggcacctc gatggcagac attgagactc cacgtgcgac agacgttgac gttcgatgca
     6061 ctaggtcgaa cgcaaacgga gggcctacgt ccgacgatca gttgaacgcg taggcacgcg
     6121 gaggtctgaa tacgtacacg cagggctggt ggcccgcggg cgcctcgggg agcgcgaagt
     6181 cattgactac gtggaattcc tcgggcgtca gcgtaggcgg caacgcgagc gcgacatcct
     6241 cggtgtcatc gactgcgtcg acgagcgcag cggctgtcgg tggggcgctc agctcgcgtg
     6301 cgtccgcggc actgacgagc agtccgaggg ttacaagcgc ggcgaggatg cgcacgacca
     6361 cgtagccact atatgtcggg cgatgccgct gtcgagaggc tacgagctcg tttgagcagg
     6421 cggggtttgc gtcgtcgtcg gtggatccga cgaggccggc cgcgttcttg gctgcttgaa
     6481 cggcaacagg acggtgaatt ccgtaaacac gtcgggctgc gaatcgacgg taatcagccc
     6541 accgtgcagc tcgatgaggc cgcgcgcgat gtagaggccg aggccagcag agtcgggcca
     6601 ggcggaggtg tggtgcttga ccggtttggg gtgggcgaac gggtcgaaga tgcgctggcg
     6661 atcttcttcc gaaatgccga cgccattatc tcgtacgcgc aaccgcgccc agccgcccgc
     6721 gtagcgctcg cgtggtgctt cgtcgacgcc gatcatgatc t
//
LOCUS       DASUFX010000652         4931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_798685,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000652 DASUFX010000000
VERSION     DASUFX010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798685"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFV99_26445"
     CDS             <1..1204
                     /locus_tag="VFV99_26445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5062953.1"
                     /translation="VEGHYRLAQRLEQRADRNAAVVELRAVLEQEPDHLDARLDLART
                     LRRLGKLEEAVAQTRSAFDRAGQPMDIAEELYWLLCEADDRQGAIDLLTLLDDDRSDA
                     DALATVAQFQRQLGRMAEARTVASHIRALDPDASTIALAETDLAEGLVEHAIEQALTV
                     PEKSKRFLQARRVVIEGLIKAKHPDRALAIVEPLRKARPDDLELAFAEAFAHAEAGDA
                     AKARELANQLKGDAMVVAFLRARILDHIGDDAGALAILEPALRANPDHVGGLNLAGYI
                     LADLPDKDVGKIGSMTRGRKERLDAAERYLAHARDLQPGDPSILDSWGWMLYQRGRTR
                     EAVRVLDRATRFSPREPEILVHLAAAWAADGAPRTAAEVLDRAEKLAPSPTVKRRIDA
                     LRAGLAIR"
     gene            1209..1517
                     /locus_tag="VFV99_26450"
     CDS             1209..1517
                     /locus_tag="VFV99_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062954.1"
                     /translation="MKRAWVFALVFAAACGKGPSDDDCKKFLDHLVDLEFKKAGAAAT
                     TDAMKQEIAKQKNTVTEAKSSEFLDVCKKKMSKARVQCALAATELDGDNGVGKCDETK
                     "
     gene            complement(1877..2902)
                     /locus_tag="VFV99_26455"
     CDS             complement(1877..2902)
                     /locus_tag="VFV99_26455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5062955.1"
                     /translation="MPQLPRSVDVAIVGGGFAGMATAWWLSRTGVHDVLVLEREAELG
                     RYASGRSAGLGRQLAEDDLMTGFTVTGAAYLRKHFSHCWTESGGILTFDKAELARGYA
                     ARADRMGIETKRLERNEVLAHWPQLNLVDIAAGLHVPSDGIIDIGKLLASYAENMRIS
                     FETSVERIIATPKGATLLTNIGSIVARVIVDATGAWAGRTTGDPLLDSYKRHVYVLEA
                     AAPQGSAPFVWHLGGSELYVRAIDGQIMVSPCDATKVEPGDQQPDDAGEALMRTRFAG
                     SPLGTVGIARAWACQRTFPERLAMKLMRDEKRPWLVWAAGLGGHGATASAAVGETVAG
                     LVIAALG"
     gene            2946..3194
                     /locus_tag="VFV99_26460"
     CDS             2946..3194
                     /locus_tag="VFV99_26460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062956.1"
                     /translation="MPRLIRLDTGDEIGTIDDKQLAFLVSQLEEEHDDDKDYFIDRDT
                     LELLSDNGADPELLALLEKGLAGDDEMDIAWDGDAKTD"
     gene            3380..>4931
                     /locus_tag="VFV99_26465"
     CDS             3380..>4931
                     /locus_tag="VFV99_26465"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5062957.1"
                     /translation="MKRLSRILALAALAAPAVGCLDDDPFDGEEVKSEDGKADASALG
                     VFLDATFNGKLVTDSSWNDRQTVQDQLLYTVGQLNGYTAVGRVDKAEITNIQKTTVNG
                     KTQITYTAKLPVIWKKSQGVPDKIDLKLPLDISYTGQDAFAEKYKHDCVDWGAHDVDA
                     GSMFYYFRPRASGCNIAAADVHAVSASVALSPAQTTGKFPEYNKIWEDNTLNVVAIFG
                     KYEDGATTAADAGIDGYNEFIGAMKSELSSRNLTTIPATVPTNPGVAAPDIEFNATLP
                     DGKKIKVVALLTDNVNTGLQNPTFRARYESLSTRADFITYNGHAGLGSNIRALASAGK
                     WVAGQYVVVFMNGCDTFAYIDGSLSSAHKAVNPDDTTGFKYIDIVNNGMPAFFANMAE
                     SSLAMFRGFLSYDDPKTYEQIFTKIDSSQLVMVTGEQDNTFTPGGGGTPTAWAGLNEN
                     ATLARNAKKTFTTPTLAAGKYQFAMTGSGDADLYVKVGSAPSMSSYDCRPYKTGSNET
                     CEVTLAQPA"
BASE COUNT          904 a         1713 c         1558 g          756 t
ORIGIN      
        1 cgtcgagggc cattatcgtc tcgcgcagcg actcgaacag cgcgccgatc gcaacgcagc
       61 cgtcgtcgag ctccgcgcgg tgctcgagca ggagcccgat catctcgacg cgcgtctcga
      121 tctcgcgcgc acgctgcgcc gcctgggcaa gctcgaggaa gcggtcgcgc agacacgcag
      181 tgcgttcgat cgggcgggcc agccgatgga catcgccgag gagctgtact ggctgctctg
      241 cgaagccgac gatcgccagg gcgcgatcga tctgctcacg ctgctcgacg acgatcgcag
      301 cgatgccgac gcgctcgcga ccgtggcgca gtttcagcgc cagctcggcc ggatggctga
      361 agcacgcacc gtcgcgtcgc acatccgcgc gctcgacccc gatgcgtcga cgatcgcgct
      421 cgccgaaacc gatctcgccg agggcctcgt cgagcacgcg atcgagcagg cgctcacggt
      481 gcccgagaag tcgaagcgct ttctgcaagc gcgacgtgtc gttatcgaag gcctgatcaa
      541 ggcgaagcac cccgaccgcg cactggcgat cgtcgagccg ctgcgcaagg cgcgtcccga
      601 cgacctcgag ctcgcattcg ccgaagcgtt cgcccacgcc gaggcgggtg acgccgcaaa
      661 ggcgcgcgag ctcgcaaacc agctcaaggg tgacgcgatg gtcgtcgcgt ttctgcgcgc
      721 gcgcatcctc gatcacatcg gcgacgatgc cggcgcgctc gcgatcctcg agcccgcgct
      781 acgggccaac cccgatcacg tcggcgggct caaccttgct ggctacatcc tcgccgatct
      841 tcccgacaag gatgtcggca agatcggatc gatgacacga ggccgcaaag agcgcctcga
      901 cgccgccgag cgctacctcg cccatgcgcg cgatctgcaa cccggcgatc cgtcgatcct
      961 cgatagctgg ggctggatgc tctatcagcg cggtcggacc cgcgaagccg tgcgcgtgct
     1021 cgatcgcgca acgcgattct cgccccgtga accggagatc ctcgtacatc tggcggcggc
     1081 gtgggctgcg gatggcgcac cgcgtacggc cgctgaggtg ctcgatcgcg ccgaaaagct
     1141 ggccccatcc ccgacggtga aacgccggat cgatgccctt cgcgccgggc ttgcgatacg
     1201 atgaccccat gaagcgcgcc tgggtgttcg ctctcgtatt cgcggctgca tgtggcaaag
     1261 gcccctcgga cgacgactgc aagaagttcc tcgatcacct cgtcgatctc gagttcaaga
     1321 aggcaggcgc tgccgcgacg accgacgcga tgaagcaaga gatcgcgaag cagaagaaca
     1381 cggtcaccga ggccaagagc tccgagttcc tcgacgtatg caagaagaag atgtcgaagg
     1441 cgcgcgtgca gtgcgcgctc gccgcgaccg agctcgacgg cgacaacggc gtcggcaagt
     1501 gcgacgaaac gaagtagcgt cgacgacagc gtgatcgcca ccgtgatcgt ggccgtgctc
     1561 gtgaacggga ccgccaccgt ggacgtgatc gacgccgtcg acgacggtca tcgcgtctcg
     1621 acccgcgaac gccccaggta gtcgcgtcgc ttgggaggtg gtcccgcgaa cgccccaggt
     1681 agtcgcgtcg cttgggaggc ggccccacgc cagcgcagga tcgactgggc tgctgatcga
     1741 cggctcggat ctcggcgcca gtcgatcacg gtgtcggcaa cgatcgcggt tgagttcacg
     1801 gtcacgagca cggccacgtc cacggggccg gccacgtcca cgtccacgat cacgttcagg
     1861 gcaacgaacg agcgcgctaa cccagtgccg cgatcacgag gcccgcgacg gtctcgccga
     1921 ctgccgccga cgccgtggcg ccgtgaccgc cgagaccggc ggcccacacg agccacggcc
     1981 gcttctcgtc gcgcatcagc ttcatcgcga gccgctcggg gaacgtgcgc tggcacgccc
     2041 acgcgcgcgc gattcccacg gtaccgagcg gtgagcctgc gaagcgcgta cgcatgagtg
     2101 cctcgccggc gtcgtcgggc tgctggtcac ccggctcgac ctttgtcgcg tcgcacggcg
     2161 acaccatgat ctggccgtcg atggcgcgca cgtacagctc gctgccgccg agatgccaca
     2221 cgaacggggc actcccctgc ggcgcggccg cttcgagcac gtagacgtgg cgcttgtacg
     2281 aatcgagcag cggatcaccg gtcgtgcggc ccgcccatgc gccggtggcg tcgacgatga
     2341 cgcgcgcgac gatcgaaccg atgttcgtca gcagcgtggc gcccttcggc gtcgcgatga
     2401 tgcgctcgac gctggtctcg aagctgatcc gcatgttctc ggcgtacgac gcgagcagct
     2461 tgccgatgtc gatgatgccg tcgctcggca cgtgcaggcc tgccgcgata tcgacgaggt
     2521 tcagctgcgg ccagtgcgcg agcacctcgt tgcgttcgag ccgcttcgtt tcgatgccca
     2581 tgcgatcggc acgcgccgcg tagccccgcg cgagctcggc cttgtcgaac gtgagaatgc
     2641 cgccgctctc ggtccagcag tgcgagaagt gtttgcgcag atatgccgcg ccggtcaccg
     2701 tgaagccggt cattagatcg tcttcagcga gctggcgccc gagtcccgcg ctacgtccac
     2761 ttgcgtagcg gccgagctct gcctcccgtt cgagcaccag cacgtcgtgc acgccggtcc
     2821 gcgatagcca ccaggcggtc gccattccgg caaaaccgcc gccgacgatc gcgacgtcta
     2881 cgctgcgtgg cagctgaggc accggagcct tgtagcacac gagcaaagtc aggcagactc
     2941 cccctatgcc acgtctgatt cgcctcgata ccggagacga gatcggaacc atcgacgaca
     3001 agcagttggc gtttctggtc tcgcagctcg aagaagagca cgacgatgac aaggactact
     3061 tcatcgatcg cgacacgctc gagctgctct ccgacaacgg tgccgatccg gagctacttg
     3121 ccctgctcga aaaaggcctc gccggcgacg acgagatgga tatcgcctgg gacggcgacg
     3181 cgaagaccga ttaaagggga ctgaccggac tgaccccttt tcgcgctgcc accggttgcc
     3241 gcgtgaggca ccggtttagc ggctgatcgg gcagccggtc gcacccgaaa ctggctactc
     3301 gcgttgcgcc gcgacgctcc tctctggcca tgattccaga gacttagctt cggcactggc
     3361 cttgctgatg ggcggggcca tgaagcgcct gagccggatc ctcgccctcg ccgccctcgc
     3421 ggcccccgca gttggttgcc tcgatgacga cccgttcgac ggggaagagg tgaagtcgga
     3481 ggatggcaag gccgacgcca gcgccctcgg cgtgttcctc gatgccacgt tcaacggcaa
     3541 gctcgtcacc gactcgtcgt ggaacgaccg ccaaacggtc caggaccagc tgctctacac
     3601 ggtcggccag ctcaacggct acacggccgt cggtcgcgtc gacaaggccg agatcaccaa
     3661 catccagaag accacggtca acggcaagac gcagatcacg tacaccgcga agctgccggt
     3721 catctggaag aagagccagg gcgttcccga caagatcgat ctcaagctgc cgctcgacat
     3781 ctcgtacacc ggccaagacg ccttcgccga gaagtacaag cacgactgcg tcgactgggg
     3841 cgcgcacgat gtcgacgccg gctcgatgtt ctactacttc cgtccgcgcg cgtcgggctg
     3901 caacatcgcg gctgccgacg ttcacgcggt gagcgcgtcg gtcgcgctct cgccggcgca
     3961 gaccaccggc aaattccccg agtacaacaa gatctgggaa gacaacacgc tcaacgtcgt
     4021 cgcgatcttc ggcaagtacg aagacggcgc caccaccgcc gcagacgccg gcatcgacgg
     4081 ctacaacgag ttcatcggcg cgatgaagtc cgagctcagc agccgcaacc tcacgaccat
     4141 tcccgcgacg gtcccgacga acccgggcgt tgccgcgccg gacatcgagt tcaacgcgac
     4201 gctgcccgat ggcaagaaga tcaaggtcgt cgctctgctc accgacaacg taaacaccgg
     4261 cctccagaac ccgacgttcc gcgcgcgcta cgagtcgctg tcgacccgcg ctgacttcat
     4321 cacgtacaac ggccacgccg gtctcggcag caacatccgc gcgctcgcct cggcgggcaa
     4381 gtgggtcgcc ggccagtacg tcgtcgtctt catgaacggc tgcgacacgt tcgcgtacat
     4441 cgacggctcg ctctcgtcgg cgcacaaagc tgtcaacccg gacgacacga ccggcttcaa
     4501 gtacatcgac atcgtcaaca acggcatgcc ggcgttcttc gcgaacatgg ccgagtcgtc
     4561 gctcgcgatg ttccgcggct tcctctcgta cgatgacccg aagacctacg agcagatctt
     4621 cacgaagatc gacagctcgc agctcgtgat ggtcaccggc gagcaggaca acacgttcac
     4681 cccgggtggc ggcggcacgc caaccgcgtg ggccggtctc aacgagaacg cgacgctcgc
     4741 gcgcaacgcg aagaagacgt tcacgacgcc gaccctcgcc gctggcaagt accagttcgc
     4801 gatgaccggc tcgggcgacg ccgatctcta cgtcaaagtc ggctcggcgc cgtcgatgtc
     4861 gtcgtacgac tgccgcccgt acaagacggg cagcaacgag acctgcgaag tgacgctcgc
     4921 gcagccggct c
//
LOCUS       DASUFX010000653         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2049508, whole genome shotgun sequence.
ACCESSION   DASUFX010000653 DASUFX010000000
VERSION     DASUFX010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049508"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..827)
                     /locus_tag="VFV99_26470"
     CDS             complement(27..827)
                     /locus_tag="VFV99_26470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062958.1"
                     /translation="MSRTLTACEARVKSTGPTSSSPMRRGLAGTVVLVSLVAAVVAAL
                     ASPITRGPAFHAYADQRELFGIQHAGDVLSNLAFLAVGLWAMRRAVTPAQQAAASAVS
                     LIAMGSAMYHFAPADITLALDWLGIGFTLGFVGAAVLGDRLGAVAEQRALFLTPILTL
                     ATIITWLVTGGTHGGTMTPYVAWQALGIVVPPLVALIAPGKIPRVPLLGAVALFALAR
                     LCAAHDAGLLDAIGVSGHSLKHVAAAGAAALALYAITSPASRAALSSS"
     gene            956..1522
                     /locus_tag="VFV99_26475"
     CDS             956..1522
                     /locus_tag="VFV99_26475"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5062959.1"
                     /translation="MDIDPSLLSADKIRPLSRLEYERMVQLGFFDVGERIELLEGVLV
                     KMSPQDWQHAAIVERLGKLLGRAIDEALAIRMGLPFAAGAYSEPEPDLAVVVDDPTLR
                     EHPSKVLLLIEVANTSLEYDRTAKLAVYAKARVPEYWVIDMKTMSVEVYTKPVRGLYR
                     RPQVLRDGDVLRPTRLPGIEIAISDLPR"
     gene            1701..>2393
                     /locus_tag="VFV99_26480"
     CDS             1701..>2393
                     /locus_tag="VFV99_26480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062960.1"
                     /translation="MRPITRETIVDRRQSQLRWSAVFGGTVLAVGLWILLQTLGMGIG
                     LAAVHTDDVGNLKGVGIGTGIWSIIAPLIALFIGAYVAGRLSASRDRKVGAVHGSVMW
                     GLAIAIGLWAMLGLVSSLASGVARVGGAAATAGSSIAAGAVKAGTSADGEGAMSALGI
                     DTNDLVAPINRRLEQEGKPAITAQQLNATVKAVAQRGLREGKLDRAALVDEVARNTAL
                     SREDAEDVANQIE"
BASE COUNT          435 a          765 c          810 g          383 t
ORIGIN      
        1 cgagaagggc gacgtcgacg gtgaggtcaa gaagatgaaa gagcagctct cgaagctggt
       61 gaagtgatcg cgtaaagcgc gagcgcggcg gcaccggccg cggcgacatg cttcaagctg
      121 tggcccgaga cgccgatcgc gtcgagtagg cctgcatcgt gcgcggcgca tagccgcgcg
      181 agcgcgaaca acgcgacggc accgagcagc ggcacgcgcg gaatcttgcc cggtgcgatc
      241 agcgcgacga gcggcggcac cacgattccg agcgcctgcc acgcgacgta gggcgtcatc
      301 gtgccgccat gcgttccgcc ggtcacgagc cacgtgatga tcgtggcgag cgtcagtatg
      361 ggtgtgagaa acaacgcgcg ctgctcggcc accgcgccga ggcgatcgcc gagcaccgca
      421 gcgccgacga agccgagcgt gaagccgatg cccagccagt cgagcgcgag cgtgatgtcg
      481 gccggcgcga agtgatacat cgccgagccc atcgcgatca ggctcacggc gctcgcggct
      541 gcttgctgcg ctggcgtcac cgcgcgtcgc atcgcccaca gtccgacggc gaggaacgcg
      601 aggttcgaca gcacgtcgcc cgcatgctgg attccgaaaa gctcgcgctg atcggcatag
      661 gcgtgaaacg cgggcccgcg cgtgatcgga gatgcgagcg ctgcgacgac tgccgcgacg
      721 agtgagacga gcacgacagt gccagcaaga ccccgccgca tcgggctgct actcgtcggg
      781 ccagtcgact tcacccgggc ctcgcatgcg gtgagtgtac gggacatggc cgcagatcgg
      841 acggcagcgg acggcacgcg gccaaaccgg acacgcgcga tcgtgatctt cgtcggttgc
      901 gcaggcacgc acatggcaat gcggcgcgac gcatggaggc gctatgctct catcaatgga
      961 catcgacccg tcgctcttgt cggccgacaa gatccgaccg ctttctcgac tcgagtacga
     1021 gcggatggtg cagctcgggt tcttcgacgt gggcgagcga atcgagttgc tcgaaggcgt
     1081 gctcgtcaag atgagcccgc aagactggca gcacgcggca atcgtcgagc ggcttggcaa
     1141 gctcctcgga cgcgcgatcg acgaggcgtt ggcgattcgc atgggactgc cctttgctgc
     1201 cggtgcgtac tcggaacccg agccggatct tgcagtcgtc gtcgatgacc caacgttgcg
     1261 cgagcaccca tccaaggtgc tgctgctgat cgaggtcgcc aatacatcgt tggaatacga
     1321 ccgaacagca aagctcgccg tctacgcaaa agcgcgtgtg cccgagtact gggtaatcga
     1381 catgaagacg atgagcgtcg aggtctacac gaaacccgta cgcgggctct acaggcgccc
     1441 tcaagtactc cgtgatggcg acgtgttgcg tccgactcgg ctgccgggca tcgagatcgc
     1501 aatctcggat ctgccgcgct agtcgcgccg ggcactcaca cgtgcgctcg cgcgcgtcga
     1561 tccggccgct gatcagtcgt ggcaccacgt gtgcggtccg taggcgcccg cattctgccg
     1621 gtgcgtcccc gctcggtagc tcgtttcgct acggtggcct ctgtagacga gcggtctatg
     1681 ccctgcatgg ttgtgttgcc atgagaccaa tcactcgaga gacgatcgtc gatcgtcgac
     1741 aatctcagct gcgctggagt gcggtgttcg gcggcacggt actggccgtc ggcctttgga
     1801 tcctcctcca gactcttggc atgggcatcg gactcgctgc cgtccacacc gacgatgtgg
     1861 ggaacctcaa aggtgtcggc atcggcaccg gaatctggtc catcatcgca ccgttgatcg
     1921 cgctgttcat cggcgcgtac gtcgccggcc gcctatcggc atcgcgtgat cgcaaagtcg
     1981 gcgcggtcca cggcagcgtg atgtggggcc tggcgatcgc gatcgggctg tgggcgatgc
     2041 tcgggctcgt gtcatcgctc gcaagcggcg tggcgcgcgt cggaggtgca gccgcgaccg
     2101 caggctcgtc gatcgccgct ggtgccgtga aggccggcac gagcgccgat ggcgaaggcg
     2161 cgatgtcggc actcggcatc gacacgaacg atctcgtcgc accgatcaac cgtcgtctcg
     2221 agcaagaggg caagcctgcg atcaccgcgc agcaactcaa cgcgacggtg aaagcggtcg
     2281 cccagcgcgg cctgcgcgaa ggcaagctcg atcgtgcagc tctcgtcgac gaggtcgcac
     2341 gcaataccgc gctgtcccgc gaagacgccg aagacgtcgc gaaccagatc gaa
//
LOCUS       DASUFX010000654        14119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1633954, whole genome shotgun sequence.
ACCESSION   DASUFX010000654 DASUFX010000000
VERSION     DASUFX010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633954"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFV99_26485"
     CDS             <1..1041
                     /locus_tag="VFV99_26485"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062961.1"
                     /translation="LDIPSRNERQRAESWASSLERYQIALSDPSELAARYRVGPGIID
                     RVCAEVARRPDRPTQPSKWVSELDDAVRQHLENRLGKTATRVTRLATWADVVLPEDIV
                     DSLLELTSRVRHRKTVFEQWGFDRSITTARGITALFQGSPGTGKTMVAGVIARDLGLE
                     VYRVDVSRITSKWIGETEKNLGSLFDAAEDGQVMLLFDEADSLFAKRTEVKTSVDRYA
                     NMEVNYLLQRLDSFEGIAILTTNFGNAIDPAFKRRLTYRVTFPFPDEEMREQLWKSLI
                     PAQVPVQGKLDFAALSQRFRLSGGYIRNAALRAAFLAAEEGVALSHEHLERAIKMEFR
                     EIGKLAETGTLE"
     gene            1132..1845
                     /locus_tag="VFV99_26490"
     CDS             1132..1845
                     /locus_tag="VFV99_26490"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX5062962.1"
                     /translation="MDVDRARETIAAEKPRRLRLDELLRDLGADDTVPPEPASAKAAA
                     ETLADGSAATPVRPEGVTVGELVDRAAEAGFGFLIGVLALIAIPFFGLSTPFGLAIAL
                     IGAQLAIGKQRPWLPKSARRRHLSMAMLDRVLRLFSKRLRWLAYSTRRRYEVVITPKW
                     IGLAITLLGLGLALPLPIPGSNMIFLIPIMIYSIALLERDGIWTVIGHVGTLIDIGLL
                     FAFGATVVAVLERAWHWIT"
     gene            complement(1856..2353)
                     /locus_tag="VFV99_26495"
     CDS             complement(1856..2353)
                     /locus_tag="VFV99_26495"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5062963.1"
                     /translation="MALSVLPLDLTRWDESARDCLVLPVFRDDRPLRGAAGLADWRLC
                     GRLSRLLKANRATGDAGETMLLPPGRRLRFKRVLWFGLGDAKGYTDDRLRKDLTWIAE
                     VVNKAGATDWAVQVPGRASGLIGARRAVEVILEDDALAGQPITLLEDPAGQKDIAELL
                     RSQRT"
     gene            complement(2353..2784)
                     /gene="nusB"
                     /locus_tag="VFV99_26500"
     CDS             complement(2353..2784)
                     /gene="nusB"
                     /locus_tag="VFV99_26500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5062964.1"
                     /translation="MSGARRTGRAYALQLLYARDGDPATDLSGAAVSWASEFELEIDP
                     AAQTFANDLVAAAVSRAAEVDELIASASKNWRIDRMSRVDRNILRLGACELLAFRDVP
                     VKVVINEAVELAKRFGTAESSAFVNGVLDRIAGAVGRSAED"
     gene            complement(2781..3275)
                     /gene="ribE"
                     /locus_tag="VFV99_26505"
     CDS             complement(2781..3275)
                     /gene="ribE"
                     /locus_tag="VFV99_26505"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5062965.1"
                     /translation="MPTTIEGSLSAAGLRFAIVASRFNGLIVEPLVAGALDAIVRSGG
                     DADNVTFVRCPGAWELPQVVRRVVERGGFDAIIALGAVIRGSTPHFDYVAGEAARGIA
                     GAAATTNIPISFGVLTTDTIEQALERAGTKAGNKGFDAALAAIELANLFRSLAGGGRA
                     KKGK"
     gene            complement(3299..4465)
                     /gene="ribB"
                     /locus_tag="VFV99_26510"
     CDS             complement(3299..4465)
                     /gene="ribB"
                     /locus_tag="VFV99_26510"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5062966.1"
                     /translation="MTSIDRVQRALEDIRAGKMVILVDDEDRENEGDLVFAAELVTPE
                     HINFMATHARGLICIAMADALIDKLELPMMVRNNEASLGTAFTVSIEARHGVSTGISA
                     RDRATTIRAAIADDAGPHSVVSPGHVFPLRARKGGVLVRTGQTEGSVDLARLAGLKPA
                     GVICEIMREDGEMARRAELEQFAAKHGLLLLSVADMIAYRLQRERIVRCQGVGTLRPG
                     ALGPGEPFTAHYYTTDVEDTEYLALVRGDVASVSAAGGTVLVRVAAMDPLADPFDLRP
                     DLVSALKAIDEAGTGVFLYVVNRSRTKLGASFERLVLQRETGRTSVVSMNQTSEALRD
                     FGLGAQVLADVGCHKIRLMMWSDRKIPGLEGFGIDVVERVPIPPPTSAKAGKTA"
     gene            complement(4462..5046)
                     /locus_tag="VFV99_26515"
     CDS             complement(4462..5046)
                     /locus_tag="VFV99_26515"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5062967.1"
                     /translation="MVEDVGTVARADRRSDALVLAIRPTQIPLTELVVGESVCHDGAC
                     LTVTDVGRDSFSVLAGAETLARTTLGNLRVGKRVNLERSLKVGDRLGGHWVTGHIDGT
                     GELAARRDLGSNLVLVVRAVPALLKYIVEKGSIAIAGVSLTVNAVDTETFSVAIIPHT
                     RDMTTLGDLAIGDRVNLETDILAKHVEKLLGGSR"
     gene            complement(5222..6331)
                     /gene="ribD"
                     /locus_tag="VFV99_26520"
     CDS             complement(5222..6331)
                     /gene="ribD"
                     /locus_tag="VFV99_26520"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5062968.1"
                     /translation="MTEKERDAAFMARALALAEKWRGRTAPNPIVGCVIVNARGEVIA
                     EGAHKGPGHKHGEIAALAKLDYKAPGATLYVNLEPCTHEGRTPPCMPVVRDSGVARVV
                     IGTEDPIPGHGGGIEALRRAGIAVSRAMVAECDAANRPFLTWAVHKRPAFTLKAAITL
                     DGKIATVKGHSKWITGEAARADVHRMRDTHDAVLVGVGTVLADDPWLTARLPRARDPI
                     RIVLDGKLRTPASAHLLPKRGGPRTIIATSADAPAAKEKVLVKAGAEVWRFATRRNGQ
                     VPLDKLARALGEENITSVLVEGGGEVHASFLSYELADELVLYVAPKVVGGPARSWVGG
                     KGIAMLHSAHQLEFVGEPIQLGADLKLTAVRKNGV"
     gene            complement(6324..6857)
                     /gene="nrdR"
                     /locus_tag="VFV99_26525"
     CDS             complement(6324..6857)
                     /gene="nrdR"
                     /locus_tag="VFV99_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5062969.1"
                     /translation="MRCPFCATDEDKVIETRVSKDGSEIRRRRECEGCKRRFTTYERI
                     EESMPAVIKSDGRREPFDRNKIEHGIRAAVAKRPVSRDQVAALALEVEREVGELGVSE
                     INARAVGERVLPKLKALDQVAFVRFASIYRDFRDLDGFVKEVDALRGEDTGPHSTPVV
                     ESEERTDEHPPVAASGD"
     gene            complement(6857..7306)
                     /gene="rpiB"
                     /locus_tag="VFV99_26530"
     CDS             complement(6857..7306)
                     /gene="rpiB"
                     /locus_tag="VFV99_26530"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5062970.1"
                     /translation="MKWYTGSDHAGFRLKQLMIAVLKELGDEVVDVGTNSEESVDYPT
                     FGQEVGKRVVADPGSFGLVICGTGIGISIAANKVKGVRAALVHDDFTAAASRAHNDAN
                     VLALGARVIGPGVAESAIRMFRSTQFVGGRHGRRVEMIEKDVPPAKP"
     gene            complement(7309..8562)
                     /gene="fabF"
                     /locus_tag="VFV99_26535"
     CDS             complement(7309..8562)
                     /gene="fabF"
                     /locus_tag="VFV99_26535"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5062971.1"
                     /translation="MRRRVVVTGIGLVTPIGSDLESTWSSLLAGKSGAGPITQFDCSD
                     YATKFACEVKGWDATKWFDKRELKHVDRFLQFGIAAAMMAVDDAGLGQKVPAGQEHRW
                     GAYIGAGLGGVKTIESTYAATQQKGPRHGFSPYFVTDIIINEAPGMVSIRTGATGANI
                     SHVSACSTGAHSIGEAVRAIRHGYADGMIAGGTEATISILGVGGFNAMRAMSTRNDAP
                     EKASRPFDKDRDGFVIGEGAGILVLEDLDSAKKRGARIYCEVTGYGATSDAHHIAAPA
                     PEHRGAQACFRVTLEDAKLDPTQIMYINAHGTSTELNDKNETFATKVVFGDHAKKLAM
                     SSTKSMTGHTLGAAGGIEAGITALAVARGVLPPTMNYETPDPDCDLDYIPNQAREQRV
                     DHAMSNSFGFGGTNAALIMSRYKGD"
     gene            complement(8594..8836)
                     /locus_tag="VFV99_26540"
     CDS             complement(8594..8836)
                     /locus_tag="VFV99_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5062972.1"
                     /translation="MSPDEIESKVKEIICNQLEVSPEQLRPEASFIDDLKADSLAVVE
                     LVLAFEQEFKITIPEEDTEQIKTVKDATNYIKTHAK"
     gene            complement(8883..9647)
                     /locus_tag="VFV99_26545"
     CDS             complement(8883..9647)
                     /locus_tag="VFV99_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEX5062973.1"
                     /translation="MAIVDKALQGRVALVTGASRGIGRAIAIALGKRGAKVIINYASR
                     EDAAREAASAVEVVGGQAAIAGFDVANAAAVTDAVKAIGKDHGSLDILVNNAGVAING
                     LLMRFSDDAWAKSINTNLGGAFHCTRAASMLLLKAKDAGRIINITSVVGEMGNGGQVA
                     YSASKAGLIGLTMSTARELASRGVTCNAVAPGFIETDMTAEHLPEAQRAKLMEQIPLG
                     RIGRAEEVADCVAFLAGPEATYVTGQVIRVNGGMLM"
     gene            complement(9657..10580)
                     /gene="fabD"
                     /locus_tag="VFV99_26550"
     CDS             complement(9657..10580)
                     /gene="fabD"
                     /locus_tag="VFV99_26550"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5062974.1"
                     /translation="MNTALLFPGQGSQRVGMGRDLALAHEVARRTYEEADEVLGFSLS
                     KLCFEGPEDDLTLTKHTQPAILTTSIAVFRALQEKGLQFDVVAGHSLGEWSALVAAGA
                     MQLRDAVRLTHLRGTFMQEAVPVGEGAMAALIGLDLAATRAVCEKASQPGSIVEPANL
                     NGGGQIVISGHTAAVERAMADAKTAGAKLAKKLPVSAPFHCSLMKPAAERLAAELANV
                     TISAPSVPVVANVTAEPTKDAGRIKDLLIQQVTAPVRWEESMQTIAKLGVVRGYELGS
                     GKVLGGLVKKIAPTIEVTTIGEPHEVAAFTA"
     gene            10632..11429
                     /locus_tag="VFV99_26555"
     CDS             10632..11429
                     /locus_tag="VFV99_26555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062975.1"
                     /translation="MRSALLLIAIAACGGSKPPPPAPPPARPAPAAQPAQPAPAPVAE
                     APPEKPAPPPEKPAPPPVAETKPLPPRKSLYERLRDTDGKVPGLPGFAIKRKVDKTYC
                     GGIKIITTRSKSVAKDDEPLAAVYKLEFPKGLSFDPDPKNDTKRKASLKKVDELITQL
                     TLVGGDARKFYEQKFTSDNALATKAVAAARLAQVYQRLASTLARAEIPLDVRTGDFVH
                     DKIEAFCDSLLDKAEPIAALAEQAATACAEKAKSVDEGWWNEVCVAP"
     gene            11450..11707
                     /locus_tag="VFV99_26560"
     CDS             11450..11707
                     /locus_tag="VFV99_26560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062976.1"
                     /translation="MRYVLLVLALAACSKSKCEKYADWEWKCGNYPADQKEITLKLAQ
                     GMCVAGENGEKGAERFAKEAECALKAKDCAEYKACGDKIEP"
     gene            complement(11704..12429)
                     /locus_tag="VFV99_26565"
     CDS             complement(11704..12429)
                     /locus_tag="VFV99_26565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062977.1"
                     /translation="MTESSNEVADQRAIEVELELARFLGFLYVVAVLLSCWLYPTAAR
                     GVKTLNEWFQKSAQRERHCDLLEQRTHTLRVEMEDDRPEVRLLAERRFRSTRSKSCVD
                     DESSSWGAHDCVLAESDGAYDACMVQQATRLEYDIAHRRRANTVAEARTFALARDEIC
                     RMNQIIADVAARERKLAAEMGYRWDGFDSGVFAAQELRVCVDEPVTTMLVERCWYAQD
                     YDCLVRLAQEVRDAIPDIPYSGP"
     gene            13524..>14119
                     /locus_tag="VFV99_26570"
     CDS             13524..>14119
                     /locus_tag="VFV99_26570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062978.1"
                     /translation="MESSGKRKRTRGPNKQTPWSRRPDAPVSVLRLALDTSDPLQRSR
                     VEQMFETAYTLKRALQRNARDRCRAYWAAPHERTKDPAAVRERLGLSRDALEDAAFAH
                     LDAAPHLRRFVTKALAMHIADAVWTGAERHLFRDGRGARQGAPRVGRWYDFTRLPGRA
                     RSHTKARKWETFRLHGSLDGHRAAYTNRDGDFVQPRRL"
BASE COUNT         2405 a         4816 c         4462 g         2436 t
ORIGIN      
        1 ctcgacattc cgtcgcgcaa cgagcgccag cgcgccgagt cgtgggcgag ctcgctcgag
       61 cgctaccaga tcgcactctc cgatccgagc gagctcgcag cacgctatcg cgtcggtccc
      121 ggcatcatcg atcgcgtgtg cgccgaggtc gcgcgccgtc ctgatcggcc gacccaaccg
      181 tccaaatggg tgagcgagct cgacgacgcc gtccgccagc atctcgagaa tcggctcggc
      241 aagaccgcga cccgcgtgac ccgcctcgcg acatgggccg atgtcgtgct ccccgaagac
      301 atcgtcgact cgctgctcga gctgacgagc cgcgtgcgcc atcgcaagac cgtgttcgag
      361 cagtggggct tcgatcggtc gatcacgacc gcacgcggca tcaccgcgct gttccaaggc
      421 agccccggta ccggcaagac gatggtcgcc ggtgtgatcg cgcgcgacct cggcctcgag
      481 gtgtaccgcg tcgacgtctc gcgcatcacg tcgaagtgga tcggcgaaac cgagaagaac
      541 ctcggctcgc tgttcgacgc cgccgaggac ggccaggtga tgctgctgtt cgacgaggcc
      601 gactcgttgt tcgcgaagcg caccgaggtg aagacgagcg tcgatcgcta cgcgaacatg
      661 gaggtcaact atctcctgca gcgactcgat agcttcgagg gcatcgcgat cctgacgacg
      721 aacttcggta acgcgatcga ccctgcgttc aaacgccgcc ttacctatcg cgtcacgttc
      781 ccgttccccg acgaggaaat gcgcgagcag ctatggaagt cgctcattcc ggcgcaggtg
      841 ccggtgcaag gcaagctcga cttcgcggcg ctctcgcaac gcttccggct atccggcggc
      901 tacatccgca acgcggcgct ccgtgctgcg ttcctcgcgg ccgaagaagg cgtcgcgctt
      961 tcgcacgagc acctcgagcg cgcgatcaag atggagttcc gcgagatcgg taagctcgcc
     1021 gagactggca cgctcgagtg agagccccct ccgcgtgatt gcgtggcctc cccgaccggg
     1081 ctggctccgc gatgtctcga catttcggac gtagtggcta ctgtttcttg gatggatgtc
     1141 gatagagcgc gagagacgat cgcggccgag aagccgcggc gcttgcgcct cgacgagctg
     1201 ctccgcgacc tgggcgccga cgacacagtg ccaccagagc ctgcgagcgc gaaggcggcg
     1261 gctgagacat tggccgacgg cagcgcggcg acgccggtgc ggcccgaggg agtgaccgtc
     1321 ggcgagctgg tcgatcgcgc cgccgaggcc ggcttcggct tcttgattgg tgtgctcgcg
     1381 ctgatcgcga tcccgttctt cggactttcg acgccgtttg gcctcgcgat cgcactgatc
     1441 ggcgcgcaac tcgcgatcgg caagcaacga ccatggttgc cgaagagcgc acgacgccgc
     1501 catctctcga tggcgatgct cgatcgcgtg ctgcgactgt tctcgaagcg gctgcgctgg
     1561 ctcgcctact cgacgaggcg ccggtacgag gtcgtcatca cgccgaagtg gattggcctc
     1621 gcgatcacgc tgctcggact cgggctcgcg ttgccgctac cgattcccgg ctcgaacatg
     1681 atcttcctca tcccgatcat gatctattcg atcgcgctgc tcgaacgcga tgggatctgg
     1741 acggtcatcg gtcacgtcgg cacgttgatc gacatcggac tgctgttcgc gttcggcgcg
     1801 acggttgtcg ccgtgctcga gcgcgcctgg cactggatca cgtaggtgcg ctggcttagg
     1861 tgcgctgact gcggagcagc tcggcgatgt ccttctgacc cgccggatct tcgagcaacg
     1921 tgatcggctg ccctgcgagt gcgtcgtcct cgaggatcac ctcgactgca cgccgtgcac
     1981 cgatcaagcc cgacgcacgt ccgggcacct gcacggccca gtcggtcgcg ccggccttgt
     2041 tgacgacctc ggcaatccac gtgaggtctt tgcgcagccg gtcgtcggtg tagcccttcg
     2101 cgtcgccgag cccgaaccac agcacgcgct tgaagcggag ccggcggccc ggtggcagca
     2161 gcatcgtctc gccggcgtcg ccggttgcgc gattggcctt gagcagtcgc gagagccggc
     2221 cgcacagccg ccagtcagcg agcccggccg cgccgcgcag cggacgatcg tcgcggaaca
     2281 cgggaagcac gaggcagtcg cgcgcgctct cgtcccatcg cgtgaggtcg aggggcaaga
     2341 ccgagagcgc catcagtcct ccgccgagcg gccaactgca ccggcgatgc gatcgagaac
     2401 tccgttgacg aacgccgacg actccgcggt gccaaaccgc ttcgcgagct cgacggcttc
     2461 gttgatcacg actttcactg gcacgtcgcg gaacgcgagc agctcgcacg cgccgaggcg
     2521 caggatgttg cgatcgacgc gcgacattcg atcgatgcgc cagttcttcg aggccgacgc
     2581 gatcagctcg tcgacttctg cggcgcgcga gactgccgcg gcgacgaggt cgttcgcgaa
     2641 cgtctgtgct gcaggatcga tctcgagctc gaactcgctc gcccagctca ccgccgcgcc
     2701 gctgagatcc gtggccggat cgccatcgcg ggcgtagagc agttgcagcg catacgcgcg
     2761 gccggtgcgg cgggctccgc tcatttcccc ttcttagcgc gaccgccgcc cgcaagcgac
     2821 cggaagagat tcgcgagctc gatcgcggcg agtgccgcat cgaagccctt gttgcctgcc
     2881 ttcgtgcccg cacgctcgag tgcttgctcg atcgtgtccg tcgtcagcac gccgaagctg
     2941 attggaatgt tggtagtcgc ggctgctccc gcaatcccgc gcgccgcttc gcctgcaacg
     3001 tagtcgaagt gaggcgtcga gccacggatc acggcgccga gcgcgatgat cgcgtcgaat
     3061 ccgccacgct ccacgacgcg gcgaactacc tgcggcagct cccacgcgcc cggacagcgc
     3121 acgaacgtga cgttgtcggc gtcaccgccg gagcgcacga tcgcatcgag ggcacccgcg
     3181 acgagcggct cgacgatgag gccgttgaaa cgactggcga caatcgcgaa gcgcaagcct
     3241 gctgcggaca gagaaccttc gatggtggtc ggcactgcgc cccaggatat atcaggaact
     3301 aggcagtctt gccggctttg gcactggtgg gcggcggaat gggcacgcgc tcgaccacat
     3361 caataccgaa gccttcgagt cccggaatct tgcggtcgct ccacatcatt aagcggatct
     3421 tgtggcaacc gacgtcggcg agcacctggg cgcccaaacc gaagtcacgg agcgcctcgc
     3481 tagtctgatt catgctgacg acgctcgttc tgccggtttc gcgctggagc acgagccgct
     3541 cgaatgacgc cccgagcttc gtgcggctgc ggttcacgac gtacaggaac acgccggtgc
     3601 cggcttcatc gatcgccttg agcgccgaca ccaggtcggg acgcaggtcg aacggatcgg
     3661 cgagcggatc catagcggca acgcgcacga gcacggtgcc acccgctgcc gagacgctgg
     3721 cgacatcgcc gcgcacgagc gcgaggtact cggtgtcctc gacatcggtc gtgtagtagt
     3781 gcgccgtgaa cggctcgccc gggccgagcg cgcccggccg cagtgtaccg acgccctggc
     3841 aacgcacgat gcgctcgcgc tgcaagcgat acgcgatcat gtcggcgaca ctgagcagca
     3901 gcaggccatg cttcgccgcg aactgttcga gctcggcgcg gcgcgccatc tcgccgtctt
     3961 cgcgcatgat ctcgcagatg acgccagcgg gcttgagacc ggcgagccgc gccaggtcga
     4021 cggagccttc ggtctggccg gtacgcacga gcacgccgcc cttacgcgcg cgcagcggaa
     4081 acacgtggcc cggcgacacg acgctgtgcg gaccggcgtc gtcggcgatc gcggcgcgaa
     4141 tcgtggtcgc gcgatcgcgt gcagagatgc cagtcgacac gccgtgccgc gcttcgatcg
     4201 acaccgtgaa cgcggtgccg agcgatgcct cgttgttgcg caccatcatc ggcagctcga
     4261 gcttgtcgat cagcgcatcc gccatcgcga tgcagatcag gccacgcgca tgcgtcgcca
     4321 tgaagttgat gtgctcgggc gtgacgagct ccgcggcaaa caccaggtcg ccctcgttct
     4381 cgcgatcttc gtcgtcgacg aggatcacca tcttgccggc acgaatgtct tccagcgcgc
     4441 gctggacgcg gtcgatcgat gtcatcgcga gcctcctagc aacttctcga cgtgctttgc
     4501 caagatatcg gtctctagat tcacgcgatc gccgatcgcg agatcaccca gggtcgtcat
     4561 gtcgcgcgtg tgcggaatga tcgcgacgga gaacgtctcg gtatcgaccg cgttcaccgt
     4621 cagcgacacg cccgcaatcg cgatcgagcc cttctccacg atgtacttga gcagcgctgg
     4681 cacggcgcgc acgacgagca ccaggttcga gccgaggtcg cggcgcgcgg cgagctcacc
     4741 ggtgccgtcg atgtggccag tcacccagtg accgccgagt cgatcgccga ccttgagcga
     4801 gcgctcgaga ttgacgcgct tgccgacgcg cagattgccg agcgtggtgc gcgcgagcgt
     4861 ctcggcgccg gcgagcacgc tgaacgagtc gcggccgacg tcggtgaccg tcaggcacgc
     4921 gccgtcatgg cacaccgatt caccgaccac gagctcggtc aggggaatct gcgtagggcg
     4981 aatggcgagg acgagcgcgt cgctgcgccg atcagcgcgt gcaacggtac ctacgtcttc
     5041 gaccaatccc gtgaacatag ctgctggggt ctaccactat ccgcctgacg acggaaccgg
     5101 gctgttcccc ggtgaaagcc ctacgatgtt gcgcgagcgg gcctcgtttc gtcacgaaat
     5161 ggggtcagct cccattttct ggttgggaca gacgacccga cgcggaaaag ggtcagaccg
     5221 gtcagacccc attcttgcga acggctgtca gcttcagatc cgcgcccagc tgtatcggtt
     5281 ccccgacgaa ctcgagctga tgcgccgagt gcaacatcgc gatgcccttg ccgcccaccc
     5341 agctgcgcgc cgggccaccc acgaccttcg gggcgacgta gagcaccagc tcgtcggcga
     5401 gctcgtacga aaggaagctc gcgtggacct cgccgccacc ctcgacgagc accgacgtga
     5461 tgttctcctc gccgagcgca cgcgcgagct tgtcgagagg cacctggcca ttgcgccgcg
     5521 tcgcaaaccg ccagacctcg gcgcccgcct tcacgagcac cttctccttg gcagcgggcg
     5581 catccgcgct ggtcgcgatg atcgtccgcg gaccgccgcg cttcggcagc aagtgcgcac
     5641 tcgccggcgt gcgcagcttg ccatcgagga cgatgcggat cggatcgcgc gcccgcggca
     5701 ggcgagcggt gagccacgga tcgtctgcga gcacggtgcc gacaccgacg agcaccgcgt
     5761 cgtgcgtgtc gcgcatgcga tgcacatccg cgcgcgccgc ctcgccggtg atccacttcg
     5821 agtggccttt gaccgtcgcg attttgccgt cgagcgtgat cgcggctttc agcgtgaacg
     5881 ctggccgctt gtgcaccgcc cacgtcagaa acggccgatt cgcggcatcg cactcggcca
     5941 ccatggcgcg cgacaccgcg atgccagcgc ggcgcagcgc ttcgatgccg ccaccgtgcc
     6001 cgggaattgg gtcctcggtg ccgatcacga cgcgcgcgac accagagtcg cgcacgacgg
     6061 gcatgcatgg cggggtgcgc ccctcgtgcg tgcacggctc gaggttcacg tagagcgtcg
     6121 cgcccggcgc tttgtaatcg agcttcgcga gcgcggcgat ctcgccgtgt ttgtgtccgg
     6181 gccctttgtg cgcaccttcg gcgatcactt caccgcgcgc gttcacgatg acgcagccga
     6241 cgatcggatt gggcgcggtg cgccctcgcc acttctcggc gagcgcgagt gcgcgcgcca
     6301 tgaaggcggc gtcgcgttcc ttctcagtca ccgctggcag cgaccggcgg gtgctcgtcg
     6361 gtcctctcct cggactcgac gaccggcgtg ctgtgcggtc cggtatcttc gccgcgcagc
     6421 gcgtctactt ccttcacgaa gccgtcgaga tcgcggaagt cgcgatagat cgatgcgaac
     6481 cgcacgaacg cgacttgatc gagcgccttg agcttcggca gcacgcgctc accgaccgcg
     6541 cgcgcgttga tctcactgac gccgagctcg ccgacctcgc gctcgacctc gagtgccagc
     6601 gctgcgacct gatcgcgcga caccggccgc ttcgcgaccg cagcgcggat gccgtgctcg
     6661 atcttgttgc ggtcgaacgg ttcgcgccgg ccatcgcttt tgatcacggc tggcatcgat
     6721 tcttcgatgc gctcgtacgt cgtgaagcgg cgcttgcagc cctcgcactc gcgccgacga
     6781 cgaatttcac tgccatcttt cgagacgcgc gtctcgatga ccttgtcttc gtcggtggcg
     6841 cagaaggggc agcgcatcag ggcttcgccg gtggtacgtc cttttcgatc atctcgacgc
     6901 gacgcccatg gcgcccgccg acaaactgcg tcgaccggaa catgcggatc gccgattcgg
     6961 cgacgccagg tccgatcacg cgcgcgccga gtgcgagcac gttcgcatcg ttgtgcgcac
     7021 gcgatgccgc agcggtgaag tcgtcatgga cgagtgcagc gcgcacgccc ttcaccttgt
     7081 tcgccgcgat gctgatgccg atgccggtac cgcagatgac gagcccgaac gagccgggat
     7141 ccgccaccac gcgcttaccg acttcctggc cgaacgtcgg gtagtcgacc gactcctcgc
     7201 tgttcgtgcc aacgtcgacg acttcgtcgc cgagctcctt gagcaccgcg atcatcaact
     7261 gcttcagtcg aaagcccgcg tggtcagagc ccgtgtacca cttcatcctt agtcgccttt
     7321 gtagcggctc atgatcagag ccgcgttcgt gccgccaaag ccgaagctat tcgacatcgc
     7381 gtgatcgacg cgctgctcgc gcgcctggtt cgggatgtag tcgaggtcgc aatcgggatc
     7441 gggcgtctcg tagttcatgg tcggcggcag cacgccgcgc gcaaccgcga gcgccgtgat
     7501 acctgcctcg atgccacctg cagctccgag cgtgtgaccg gtcatcgatt tcgtcgacga
     7561 catcgcgagc ttcttcgcgt gatcgccgaa cacgaccttt gtcgcgaacg tctcgttctt
     7621 gtcgttgagc tcggtcgagg tgccgtgcgc gttgatgtac atgatctgcg tcgggtcgag
     7681 ctttgcgtcc tcgagcgtga cgcggaagca tgcctgcgca ccgcgatgtt ctggtgccgg
     7741 cgccgcgatg tgatgtgcat cgctcgtcgc gccgtaaccc gtcacttcgc agtagatgcg
     7801 cgcgccgcgc ttcttcgcgc tgtcgagatc ctcgagcacg agaatgccgg cgccttcgcc
     7861 gatcacgaag ccatcgcgat ccttgtcgaa cggacggctc gccttctcgg gcgcgtcgtt
     7921 gcgcgtcgac atcgcgcgca tcgcattgaa gccgccaaca cccaagatcg agatcgtcgc
     7981 ctcggtgccg ccagcgatca tgccgtcggc gtagccgtgg cgaatcgcgc ggaccgcttc
     8041 accgatcgag tgcgcgccgg tcgagcacgc cgacacgtgc gagatgttcg cgccggtcgc
     8101 accggtgcga atcgacacca tgcccggcgc ttcgttgatg atgatgtcgg tgacgaagta
     8161 cggcgagaac ccgtggcggg gtcccttctg ctgcgtcgcc gcgtacgtcg actcgatcgt
     8221 cttcacgccg ccgaggcccg caccgatgta cgcgccccag cgatgctctt gcccggccgg
     8281 caccttctga ccgagccccg cgtcgtccac cgccatcatc gctgcggcga taccgaactg
     8341 caggaaccga tcgacgtgct tgagctcgcg cttgtcgaac cacttggtgg cgtcccagcc
     8401 cttcacttcg cacgcgaact tcgtcgcgta atcggagcaa tcgaattgcg tgatcggtcc
     8461 tgctccgctc tttccagcga gcagcgacga ccacgtcgac tctagatcgc ttccgatcgg
     8521 agtgacgaga ccgatgccgg tgacgacgac acgacgacgc attcgcatct cctaccgcgg
     8581 ccggcgccgc gtgtcacttc gcgtgggtct tgatgtagtt cgtagcgtcc ttaacggtct
     8641 tgatctgctc ggtatcttcc tcaggaatcg tgatcttgaa ttcctgctcg aacgcgagca
     8701 ccagctcgac aactgcgagc gaatccgcct tcaaatcgtc gatgaacgac gcctcgggac
     8761 gaagctgctc gggggacacc tcgagctgat tgcagatgat ttccttgacc ttgctctcga
     8821 tctcgtcagg cgacatggcg gcgttgatag tccattcccg cggccacatc aacagcccga
     8881 catcacatca acatcccgcc gttcacccgg atcacctgcc ccgttacgta ggtggcctct
     8941 ggtccggcca ggaacgcgac gcagtccgcc acctcttcgg cacgcccgat gcgcccgagt
     9001 gggatctgct ccatcaattt ggcgcgctgc gcctcgggta ggtgctcggc ggtcatatcc
     9061 gtctcgatga acccgggtgc taccgcgtta cacgtaacgc cgcgcgaagc caattcgcgc
     9121 gcggtcgaca tcgtgaggcc gatgagaccg gccttgctgg ccgaatacgc gacctgaccg
     9181 ccattcccca tctcccccac cacgctggtg atgttgatga tccggccggc gtccttcgcc
     9241 ttcagcagca acattgacgc agcgcgtgtg cagtggaacg cgccgcccag gttcgtgttg
     9301 atgctcttcg cccacgcgtc atccgagaac cgcatcaaca ggccgttgat cgccacgccc
     9361 gcgttgttca cgaggatgtc gaggctgccg tggtccttgc caatcgcctt caccgcatcg
     9421 gtcacggcgg ctgcgttcgc gacgtcgaag ccggcgatcg ccgcctgccc gcccaccact
     9481 tcgacagctg acgctgcctc gcgcgcagca tcttcgcgcg acgcgtaatt gatgatcacc
     9541 ttcgcgccgc gcttgccgag cgcgatcgcg atcgcacgcc cgatgccgcg tgacgcaccg
     9601 gtcacaagtg caactcttcc ctgcaacgct ttatcgacga tcgccatggc ttctccttac
     9661 gccgtaaacg cggcgacctc gtgcggctcg ccgatggtgg tgacttcgat cgtaggcgcg
     9721 atcttcttga cgagaccgcc gagtactttg cccgagccaa gctcgtagcc gcggacgacg
     9781 ccgagcttcg cgatcgtctg catcgattct tcccagcgca ccggcgccgt cacctgctgg
     9841 atcaacagat ccttgatgcg ccctgcgtcc ttcgtcggtt ccgctgtcac gttcgcgacg
     9901 accggcaccg acggcgcgct gatcgtgacg ttcgcgagct ccgccgcaag tcgctccgcg
     9961 gccggcttca tcagcgaaca atggaagggt gcgctcaccg gcagcttctt cgcgagcttc
    10021 gcgcccgctg tcttcgcatc cgccatcgcg cgctcgactg cagccgtgtg accgctgatc
    10081 acgatctgac caccgccgtt caagttcgcg ggctcgacga tcgagccggg ctgcgacgcc
    10141 ttctcgcaca ccgcgcgcgt cgccgcgaga tcgagaccga tcagcgcggc catcgcgccc
    10201 tcaccgacgg gcaccgcttc ctgcatgaac gtaccgcgca ggtgggtcag ccgcacggca
    10261 tcgcgcagct gcatcgcgcc cgccgcgacg agcgcgctcc actcgccgag cgagtgaccg
    10321 gcgaccacgt cgaactgcag acccttctcc tgcagtgcgc ggaacactgc gatcgacgtc
    10381 gtcaggatcg cgggctgcgt gtgcttggtg agcgtgaggt cgtcttctgg cccttcgaag
    10441 cacagcttcg acagcgagaa gccgagcacc tcgtcagctt cttcatacgt gcggcgtgca
    10501 acctcatgag cgagcgcgag gtcacgtccc ataccgacac gctgcgagcc ctgcccggga
    10561 aacaacaatg ccgtgttcat ggcgggggtt tcgcacggcc ggcgcaacct cacaagtagt
    10621 agggtccctg catgcgtagc gcgcttctcc tgattgcaat tgcagcctgc ggtggaagca
    10681 aaccgccgcc gccagcgcca ccacctgcgc ggcccgcacc ggctgcacaa cctgcgcagc
    10741 cagcgcccgc gcccgtcgcg gaagcgccgc cggagaaacc agctccgccg ccggagaagc
    10801 cagctccgcc gcccgtggcg gaaacgaagc cgcttccacc gaggaagtca ctctacgagc
    10861 ggctccgtga cacggatggc aaggtgcctg gtctgccggg cttcgcgatc aagcgcaaag
    10921 tcgacaagac gtactgcggt ggcatcaaga tcatcacgac gcgcagcaag tccgtcgcca
    10981 aggacgacga gccgctcgcg gctgtctaca agctcgagtt tccgaagggc ctctccttcg
    11041 atccagatcc gaagaacgac acgaagcgca aagcgtcgct gaaaaaggtc gacgagctca
    11101 tcacgcagct cacgctcgtc ggcggcgacg cccgcaagtt ctacgaacag aagttcacgt
    11161 ccgacaacgc gctcgcaacg aaagccgttg ctgcggcgcg actcgcgcag gtctatcaac
    11221 gcctcgcgtc gacgctcgcg cgcgccgaaa tcccgctcga tgtccgaacc ggcgatttcg
    11281 tgcacgacaa gatcgaagcg ttctgcgact cgctgctcga caaagcggaa ccgatcgcgg
    11341 cactcgccga acaagcagca acggcctgcg cggagaaagc aaagtccgtc gacgagggct
    11401 ggtggaacga ggtctgtgta gcgccgtgaa cccgtagtag gttcgccaca tgcgatacgt
    11461 tctcctcgtg ttggcgttgg ctgcgtgcag caagtcgaag tgcgagaagt acgcggactg
    11521 ggaatggaag tgcgggaact atcccgccga ccagaaagag atcacgctca agctcgcgca
    11581 gggcatgtgc gtcgcgggcg agaatggcga gaaaggcgcg gagcgcttcg cgaaggaagc
    11641 cgaatgcgcg ctcaaggcga aagactgcgc cgagtacaaa gcctgcggcg acaagatcga
    11701 gccctaaggt cccgagtacg ggatgtcagg aatagcgtct ctgacctcct gcgcgagccg
    11761 cacgaggcag tcgtaatctt gggcgtacca gcaccgctcc acgagcatcg tggtcaccgg
    11821 ctcgtcgaca cagacgcgca actcctgggc cgcaaataca cccgaatcga acccatccca
    11881 tcggtacccc atctccgccg cgagcttgcg ttctcgtgcc gccacgtccg cgatgatctg
    11941 gttcatcctg cagatctcgt ctcgagcaag cgcgaacgtg cgtgcctcgg cgacagtgtt
    12001 ggcacgacga cggtgcgcaa tgtcgtattc gagtcgcgtg gcctgttgaa ccatgcaggc
    12061 atcgtaagcg ccatcggact cagcaaggac acaatcgtga gcaccccagc tgctgctttc
    12121 gtcgtccacg cacgatttgc tgcgcgtcga gcggaagcgc cgctcagcaa gcagtcgcac
    12181 ttcaggacga tcgtcttcca tctcaacgcg cagggtgtgc gttcgctgtt caaggagatc
    12241 acaatgccgt tcgcgctgcg cgctcttctg aaaccactcg ttcagagtct tcacgccgcg
    12301 cgcagctgtc gggtagagcc agcaggacag caagacagca acgacgtaca gaaagccgag
    12361 gaaccgcgcg agctcgagct cgacctcgat cgcacgctgg tcggctacct cgttacttga
    12421 ttcggtcatg ggcgccgcta tcgatcggaa cttgccgtgg ttgcgcgcca acctgaggtg
    12481 ccggcttgtc accctcgggc tgcagcgtgc cgcggctaac tgcgcgaccg cccacgcgcc
    12541 gcgcgcggca ccgcatctgc actacgagcg aaccgtgaca acggaagact cgctcgcggc
    12601 ccgtggctcg gcaacgccat ggaggctttg cgcctgctag tgcaggcctg acggttcggc
    12661 cgcggcgctg cttgatcgcc catcggaacg gcttcccgat cggtgacagc agcgccgcgg
    12721 ctcggaacca aggggctcga tgtcgtccgc taagggttgt cccgcaaatc gagctgaaga
    12781 ttgtgaaggt aggcgacagc gcgaagtgtg tcgtgcaggt gctggccacg acgtcgatga
    12841 tctcgcagga cgcgccagac cttgagccgg acgatcgcat gttcgacgcg tgcgcgccgc
    12901 cggcgatgcg accgccacgc gcggtcacgc acgatgcgac gaacttcgaa tctgggtatc
    12961 acgagctcgg gtacgccgcg atagccgcca tctgccacag cacccgaccg tgtgccttgc
    13021 acagtcgttc catcaccgag cctcggtagt gcatcggatc gttgcgattg cctggaccac
    13081 ccgttgagat cgcgacgact cggagatcgc gacggcgtac gagcagctgc gcgttgcacg
    13141 accagcgata gttcttcgat cgcgccgcgc aggtgtggtc tcgcgtcgga atcagcgtgc
    13201 catcgacaat ccacgaccag cgtcgatcgg cgcgaactcg gtcacctaga gaggagagcc
    13261 ggtgcgtgat cgtggcgacg atccgatgcg cactggactt cgagatccca aacaccgccg
    13321 cgagctcgcg gatcgtgagc ttggtgcgta gcgcggcaca cgctatgaga atgcgatcgc
    13381 gaagcgagcg cgaccacggc cgaccgcgcc ggcactccgc cggcatgcgg tgcagtaggg
    13441 ccgcgcgtcg acgcgtactg atcgaagaga gcacgcgcat acgtgggatc gtcattgtca
    13501 gaagcgtggc gtacgatcga ttcgtggagt cgtcgggaaa gcggaagcgg acgcgtggtc
    13561 cgaacaagca gacgccgtgg tcacggcgtc ccgatgcccc ggtgtcggtc ctgcggctcg
    13621 cgctcgatac cagcgatccg ctccagcgct cgcgcgtcga gcagatgttc gaaactgcct
    13681 atacgctcaa gcgagcgctc cagcgcaatg cccgtgaccg ctgtcgcgcg tattgggccg
    13741 caccgcacga gcgtacgaag gatcccgcgg cggtccgcga gcgactgggc ctgtcacggg
    13801 acgcgctcga ggatgccgcc tttgcacatc tcgatgccgc gccacacctg cggcggttcg
    13861 tgaccaaggc gctcgcgatg cacatcgccg atgcggtgtg gacgggcgcc gagcgccacc
    13921 tgttccgaga tggccgtggg gcgcggcagg gcgcaccgcg cgtcggtcgt tggtacgact
    13981 tcacgcggct acccggccgc gcgcgctcac acacaaaagc ccgcaagtgg gagacgtttc
    14041 gcctccacgg ctcgctggac ggccatcgcg ccgcgtacac caaccgcgac ggcgacttcg
    14101 tccagccacg ccggctcca
//
LOCUS       DASUFX010000655        16164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_244394,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000655 DASUFX010000000
VERSION     DASUFX010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244394"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFV99_26575"
     CDS             <1..460
                     /locus_tag="VFV99_26575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5062979.1"
                     /translation="VWRMACAQGESHVADLVQDSLLRVLQALPRFNLAGPARISTWIL
                     TITTRVVLNDRRRVTPRDAKLEPSAYIDPATAAADVELGAAIAKAVLALPEQQRIAFV
                     LREYHDLDYGEIAEMLDVDVGTIKSRLSRARAAVRDTLTRTLPDLFRGVR"
     gene            601..1302
                     /locus_tag="VFV99_26580"
     CDS             601..1302
                     /locus_tag="VFV99_26580"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5062980.1"
                     /translation="MKWLFAALAIANIALAAIVVVLALRPPQERTVTIQRAMGGPLDA
                     QTNEILRRLERDQRELEGKLAEVQQLRTLVAQLSERVRQCDETDKRDRTVPKQRREPP
                     EPAEVTDTGTCDEVSCVLTNYEGACCAKLRTGKVPPLAPSPASQLPEMLDRQNISNAI
                     AAVRTRVSACALKSPAKGKVKVSVRVDASGRVTSVAVVSTPDPVLGACVASAIQSAKF
                     APTQQGGSFSYPFIF"
     gene            complement(1310..1957)
                     /locus_tag="VFV99_26585"
     CDS             complement(1310..1957)
                     /locus_tag="VFV99_26585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062981.1"
                     /translation="MATLTDYFVQYGDVIGYAVVGVIALIALGVIQLFRRRGDLKRAR
                     TAVRLVTYSICEPRQGPVAISGTYRETQSESWIECRGQRVSLEGGVDVVAGTRAQWRN
                     GTRTYRLRSGDEVYAIGVMTRLQGSNWKIVPSPEETGVQLYAATPRPAPAPLWPWRAP
                     LILAITGGIAFFGLYKTGEMLVDKPVCGERMQLQIAAAMPLVREDALAKLRQCPP"
     gene            complement(1970..3100)
                     /locus_tag="VFV99_26590"
     CDS             complement(1970..3100)
                     /locus_tag="VFV99_26590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062982.1"
                     /translation="MLVVEDDTDLRDALCETLADSGHVVLTAVDGQEGLRQLRATRPD
                     VVVLDLMMPGLDGWQFRLAQRKDPVLATIPVVAISGSSNPAAAAVDAELFLRKPIDGN
                     MLSKAIEQVIAERDKRLAPVKAAEVDRLATLGTLAAGLAHEINNPLTYVLIRLTQIVR
                     MLPAVDRTSDNPRVHELQELARGALEGAERIRAIMSAIRTFSRIDDIAMKATDVCVPL
                     ESALKLIANELGHRARVVKQYEEHPVVMANEGRLGQVFLNLLTNAMHAVPDGAPEQNE
                     VRVVTSTDSAGHLVVEITDTGSGMPPHVLERVFDPFFSTKPVGQGTGLGLSISRNIIA
                     QHGGTIGVTSEPDHGTTFRITLPALVPAGVSQKVQGPTRTPT"
     gene            complement(3160..4455)
                     /locus_tag="VFV99_26595"
     CDS             complement(3160..4455)
                     /locus_tag="VFV99_26595"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5062983.1"
                     /translation="MSRILVIDDDGTARSMATTTLRGHGHDVVAVDSSQAIGLLTETA
                     FDLVVTATTSDASELRDRVAAPVELVRLRPPVTERDLVVIVDQHEQRATTHETFAHAR
                     RALTEDLDPALPIIGRSPAIELVLARLSAIAVTNAPVLLTGESGTGKELLAHLIHERS
                     SRRNEALVIVNCAAFPETLIEAELFGHERGAFTGAMQKRDGKFKAAQGGTLFLDEING
                     LSLAAQAKLLRVLQDGRIQPLGTNAEIVVDVRLISATNRELSAMVADGSFRGDLYYRI
                     KVLDLDVPPLRERTGDLPLLVEHFLQKHAQPGRRPTLSPRAWAMVSTYSFPGNVRELE
                     HAIQRAIVLAGGCEIDLEHLPREIVPTLKAIPPSREREIKPLADALHAFEREYLRHAL
                     EATQGNKTQAARLLGISRKHLWEKLRQLAALESNHRHGA"
     gene            4691..5032
                     /locus_tag="VFV99_26600"
     CDS             4691..5032
                     /locus_tag="VFV99_26600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062984.1"
                     /translation="MRVLVIDDDDSLRAMISELLIEIGYELVGFASLDEVVDNASFRI
                     GRDDLILIDLDARRAAELRAPLTSAFPYTVAITVGDGDYEAIPRVQLRKRARRARASQ
                     RARATRATGAN"
     gene            5247..6875
                     /locus_tag="VFV99_26605"
     CDS             5247..6875
                     /locus_tag="VFV99_26605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5062985.1"
                     /translation="MSRDFICVSGDLGIDTLEELLLERDLSGVPVIDHERRLIGYATM
                     TDIMREHHDRPDPAPAERTEWGGHTESAPKTVAEVMTPVALELQEQSTVAIAIELMTS
                     RGVHRVPVVSNEGVLVGMITADAILRSFAKRATKVHVDEHTAEADRVVSLGFMTSGLA
                     HQVNNALTPMRLSLGRLTSFELSRRPLSPERMHRVELLQDVREGLERIERIVRALKVF
                     SHADEKESHPVDVCDALETALAVANHEIRHRAKLVRDVNPMPLVDARAEDLRQVFINL
                     LINAVHAIPEGEAHVHEIRVTTWTNARGRAAVEIKDSGTGIPVEALPRIFEPFFTTHP
                     GRALGLGLAVTRDLVASFGGEIEVDSVVGRGTAIRITLPPSEKTAQTSIAPHDERGRE
                     AEARTRLRILIVDDDRPVAAAIALELGEHDVIVAESGREALEILRRDKDFDVILCDLM
                     MPEVTGMDVYESLRLIAPALVGRVVLMTGGAFTHRAGEFLSTVNAPVLEKPFESGQLQ
                     AIVNAIERQHELAEAPILAADYAVTTPVIGGREN"
     gene            6878..7105
                     /locus_tag="VFV99_26610"
     CDS             6878..7105
                     /locus_tag="VFV99_26610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062986.1"
                     /translation="MAEQHEDPERLPDGVPHIVVGLTAACAVVLAIVLVLAWSHERIS
                     VIVLAAVVTPVLVSKLVQKSERERDHVHPSR"
     gene            7394..7825
                     /locus_tag="VFV99_26615"
     CDS             7394..7825
                     /locus_tag="VFV99_26615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5062987.1"
                     /translation="MLHNRSAPHARVVPVLTVDNVRAAVTWYATVFGFVEHVQIGEGH
                     RAQLGLPGGGELIVAEVRPGRRTPSAGRSHQVMLKVENAAAVVALARENGATIVGEPH
                     DWEFGERQATIDDVFGHQWVLTQTLVDTAPEQWGGKTVTPR"
     gene            complement(7812..9152)
                     /locus_tag="VFV99_26620"
     CDS             complement(7812..9152)
                     /locus_tag="VFV99_26620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5062988.1"
                     /translation="MQAGDGTLIEASRRGDRDAFAQIIERYQRAVYAVAFSGVRDRAL
                     ADDVAQDTFVIAWRRLAELRDEQRLAAWLCGIARNRARDMRRQAHRETVGDVDDVTHA
                     TTPYDQMTEAESERAVAAALALVPDVYREPLVLYYYEERSIADVARSLGISAATTNKR
                     LSRGRRYLAERVATVERGLARRGPRPGFAASVLALVGVTASASHVDASPAVKGSTMHK
                     LAIAAAVTATLGGAAALVLTTTHSDAHAEPHAAAAHAALDNEHAAKGATAAHSHDGSL
                     CDVAKRVQAHAPSLAPLIQSGSMAESNDCAAVGRHLAELQADTTHGPNAQPDEESFET
                     CGAHYAANCESEHWSVDQRNCTLAAGDLINAHLCAGQVPQQAPTSVPANLSCAVLGPQ
                     IAATIQAAGMHEDVTDLPQQVEAACDVGSWSMELRTCFAAASSIDTLKGCFSGE"
     gene            complement(9234..10316)
                     /locus_tag="VFV99_26625"
     CDS             complement(9234..10316)
                     /locus_tag="VFV99_26625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062989.1"
                     /translation="MRLWAIACGLWLVACGSTSSGLDDDDGSGGGGNPRNDAAIDPNG
                     GDGGGGGGGGGGGSGDGGGGGGGSGSDAATTLQPLGNGGPGIEILRTDTTHLYWARND
                     SSAIAIQRMPLAGGAIETLYTAPGWVLGLQVVGGVVYVAVTRDALNNYVGSIVSFPVT
                     GGTPTTVATPMGVRGLVVDGGWIYYGDTLASTTGEARILKMPTTGGNPHFIARARANA
                     TPYEMSIDAGILYFTDSLGVVQVEVQTKQITDLVTNMIAPQMTIDDQYVYFVGCTGTC
                     GYATAYRVPRAGGAVEPLGMTPHRLRSGIDAIDDSLQWGSYVLSTTGGPETTLLDSQD
                     PFAVVATPPAFYFANKDTGDIYRVAR"
     gene            complement(10418..11272)
                     /locus_tag="VFV99_26630"
     CDS             complement(10418..11272)
                     /locus_tag="VFV99_26630"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX5062990.1"
                     /translation="MSENRRELESNMHLKLSKGSTYGDYLHLDELLAAQRPVTAVHDE
                     MLFIIQHHTSELWIKLLLHELDLAVKLVRSDRLVETFKIFARVAHVQRMLFEQWAVLE
                     TMTPSEYLQFRDALGVASGFQSYQYRALEFVLGNKDANTLKPHAHDPHIHAELAARLA
                     APSLYDEFLRWLARKGYAIPQDRIERDFTQPYTRSAAVLEVFRTIYAEPERHYDAYDM
                     CEKLVDVEERFQLWRFRHMTTVKRIIGFAQGTGGSSGVGFLKQALELTFYPELWDVRT
                     HLAAPTRR"
     gene            complement(11435..12352)
                     /locus_tag="VFV99_26635"
     CDS             complement(11435..12352)
                     /locus_tag="VFV99_26635"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5062991.1"
                     /translation="MSRAGPNLHHIDWDDLRTALSLGRAGSIRKAARELGVSHSTVLR
                     RLHSLETAVGVQLFVDKGEGYEATPAGQDVFETATTLEETVTNLERRVSGQDLRLTGP
                     VRVTVPDPFAPLLFPIFAELAKAQPGIDVTVALGTGFVDLAHRAADVAIRVAADPPPE
                     LVGHRVCKAGVGIYGSESYLRGRNTKDLEALDWVGWEAGSEMAFARWIEKNVPRARVA
                     VRVTTGWGLREGIDAGAGVSIIPCALGEVMPAWRRVRLMNEASAPLWVLTHRDLRTTT
                     RVRVLRDFIVNALRAKRALIEGTRASRRK"
     gene            12398..12898
                     /locus_tag="VFV99_26640"
     CDS             12398..12898
                     /locus_tag="VFV99_26640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5062992.1"
                     /translation="MQTHSIANARTSTEVMVLGPGQGEDVWFTDNLLTIKARPATGTQ
                     FGVIESTLPEGSHTPFHRHDHEDEAFYVLEGRMKIYVDGRAPIEVGPGCYVHTPRGAA
                     HGFVTLTPVRMLVLCGVEGFVEMAREAGMPAPRHELPPAGPPDIARLEAACARNHITL
                     LGPLPG"
     gene            complement(12899..13291)
                     /locus_tag="VFV99_26645"
     CDS             complement(12899..13291)
                     /locus_tag="VFV99_26645"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5062993.1"
                     /translation="MDGIMRFPTAVKHEPAIDAWLRAQRDDLRPFVETWFARMRQCGS
                     DVRELMHDGMPTACVGEAAFGYVNAFRDHVNVGFFFGALLDDPARLLEGTGKRGRHVK
                     LRPGQPIDAAALAKLVDTAYADIRARLR"
     gene            13351..14103
                     /locus_tag="VFV99_26650"
     CDS             13351..14103
                     /locus_tag="VFV99_26650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062994.1"
                     /translation="MTRFAAMSWLTSAVTVVAALGTAVLRAIGSESWRPALAVTLAVF
                     GVRVAVMAWGASRRELPWSRLLLPGIIWLEGAGLATSSAATLWQVRLGTAVMLELGFV
                     VVAIREVRRVSASNEPVDDRLARAFGALLPPGAARFVAFELVIIGSALRFVIGGWRRA
                     VPAGFTYHRESVLRLFLQFLPILGVADLLLVELVLLPHAATWLRLVVHGLAIYGLVWL
                     VGFYASLRGRPHQLANGRLILHRGMLRRSPCP"
     gene            14100..14351
                     /locus_tag="VFV99_26655"
     CDS             14100..14351
                     /locus_tag="VFV99_26655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5062995.1"
                     /translation="MTDIASVEELPVFADDWKKRAYRKGAIRIDVPGPTVLELRLRTS
                     ARPFGVFGNGAASTRVLVSVDEPASFIAAVSGTAEPAHD"
     gene            14421..>16164
                     /locus_tag="VFV99_26660"
     CDS             14421..>16164
                     /locus_tag="VFV99_26660"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX5062996.1"
                     /translation="MKLPRPSAQLTSTLTELSAILRQPAVWTPHARLVGRKLPMIVKG
                     LRMIGKTDKTEALSIGSLIEQNAKLRPNAAAILYEDRRYSARDVHEMSNRWANWLAAR
                     GVGKGDVISVLLENRPEALLIIGGIVKLGAIAAVINTKQRGRTLQHSLAVAKARLHLV
                     GEELWDSFVEVRDAIGASDNVAWVREQTSARVPAGAIDAAAAVANASLTTPAALADVR
                     LGDPAFYIYTSGTTGLPKASIMSHNRWTKAAGAFGMSALAVTEADVIYLALPLYHNNA
                     LTVAWASAVSGGAALAIRRKFSVSNFWEDIRKFRATAFIYIGELCRYLLNAEPRPDDR
                     KHSVRAICGNGLRPDIWKAFKTRFGIDEVYEFYAASEGNIAFVNLLNLDATVGLCPAP
                     YALVKYDIDRDEPLRGPDGHMVRVGRGEIGLLIGEVTERYAFDGYTDKAASEKKLFRD
                     VFRKGDVWFNSGDLLRDQGFRHAQFIDRVGDTFRWKGENVSTNEVAEALNTFHQVAES
                     TVYGVQVPGTDGRCGMAALVLRCAVNDFDLRGFTRHVESQLPAYARPLFLRVRPELEV
                     TGTFKQVKSDLRRQG"
BASE COUNT         2760 a         5251 c         5286 g         2867 t
ORIGIN      
        1 cgtctggcgg atggcgtgcg cgcaaggcga gagtcacgtc gccgacctcg tccaggactc
       61 gctactccgt gtgttgcaag cgctgccgcg tttcaacctc gccgggccag cgcggatttc
      121 gacgtggatc ctcacgatca cgacgcgtgt cgtgctcaac gatcgacggc gggtcacacc
      181 ccgtgatgcg aagctcgaac cgtcggcata catcgatccg gcgacggctg ccgcggacgt
      241 cgagctcggc gccgcgatcg cgaaggccgt tctcgcgctc cccgagcagc agcgcatcgc
      301 gttcgtactg cgcgagtacc acgatctcga ctacggcgag attgcagaaa tgctcgatgt
      361 cgatgtcggc acgatcaagt ctcggctctc gcgcgcgcgt gctgcggttc gcgacacgct
      421 gacgcgcaca ctccccgatc tgttcagagg tgtccgatga atcacgacga cgatgcgggc
      481 gaagacgccc tcgcacgaat caatctcgag gcctggcgcg ttccgccacc gtccgctgca
      541 gatcggccgg cgattctctt gcgcgcattg acgccagcag ccgcgccgaa gcgtacgcgc
      601 atgaagtggt tgttcgctgc gctggcgatc gcgaacatcg cgctcgcggc gatcgtcgtc
      661 gtcctcgcgt tgcgaccacc ccaggagcgc accgtcacga tccagcgtgc gatgggaggg
      721 ccgctcgacg cgcagaccaa cgaaatcctg cgccggctcg agcgcgatca gcgtgagctc
      781 gagggcaagc tcgccgaggt ccagcaactg cgcacgctgg tggcacagct ttccgagcga
      841 gtccgccagt gcgacgagac cgacaagcgc gatcgcaccg taccaaagca gcgccgtgag
      901 ccgcccgagc ccgccgaggt caccgatacc ggcacgtgcg acgaggtctc gtgcgtcctc
      961 acgaactacg aaggcgcgtg ttgcgcgaaa cttcgcaccg ggaaagttcc accactcgcg
     1021 ccatcaccgg cgagccaact gccggagatg ctcgaccgcc agaacatctc caacgccatc
     1081 gccgctgtgc gcacacgcgt ctcggcttgc gcgctgaagt cacctgcgaa gggcaaggtg
     1141 aaggtttcgg ttcgcgtcga cgcgtcgggg cgcgtcacta gcgtggcggt cgtgagcacg
     1201 ccggacccgg tgctcggcgc gtgtgtcgcc tcggcgatcc agagcgcgaa gttcgcgccg
     1261 acccagcagg gcggcagctt ctcgtacccg ttcatcttct gaacgtcgac tacggcggac
     1321 actgccgcag cttcgcgagt gcgtcctcgc gtacgagcgg catcgcagca gcgatctgca
     1381 gctgcattcg ttcgccgcac accggtttgt cgacgagcat ctcgccggtc ttgtacagac
     1441 cgaagaacgc gatgccaccg gtgatcgcaa ggatcagcgg cgcacgccat ggccacagtg
     1501 gtgcgggcgc aggccgcggc gtcgcggcgt agagctgaac tccggtttct tccgggctcg
     1561 gcacgatctt ccagttcgat ccttggaggc gcgtcatcac gccgatcgcg tacacctcat
     1621 ctccgctgcg cagtcgatac gtgcgcgtgc cgtttcgcca ctgggcgcgc gtgccagcta
     1681 cgacgtcgac accaccttcg agcgacacgc gttggcctct gcactcgatc cagctttcgc
     1741 tctgcgtctc gcgatacgtg ccagagatcg cgaccggtcc ttggcgcggc tcgcagatcg
     1801 aatacgtcac gagccgcacc gccgtacgcg cgcgcttcag atcaccgcgc cgccggaaca
     1861 gctgaatcac accgagcgcg atcaacgcga tcacgccgac gaccgcatag ccgatcacgt
     1921 caccgtactg gacgaagtaa tcggtcagcg tcgccatagg cgcagtctct catgtgggtg
     1981 tgcgtgtggg cccttgcact ttctggctga cacctgcagg taccagcgct ggcagcgtga
     2041 tccggaatgt cgtgccgtga tcgggttcgc tggtcacccc gatagttccg ccgtgttgag
     2101 cgatgatgtt tcgacttatc gagagtccga gcccggtccc ctgaccgacc ggtttcgtcg
     2161 agaagaacgg gtcgaacaca cgctcgagta cgtgcggagg catgccgctg ccggtatcgg
     2221 tgatctcgac gacgaggtga ccggcggaat ccgtactggt gacaaccctg acctcgttct
     2281 gctccggtgc gccgtcgggc actgcgtgca tcgcgttggt cagcaggttg aggaagacct
     2341 gcccgagccg accttcattc gccatgacaa caggatgttc ctcgtactgc ttcacgacac
     2401 gggcgcggtg ccctagctcg ttcgcgatga gcttcagtgc cgattcgagc ggtacgcaaa
     2461 cgtcggtcgc tttcatcgcg atgtcatcga tacgcgagaa cgtccggatc gcgctcatga
     2521 tggcgcgaat gcgctcggca ccttcgagcg cgccgcgtgc gagctcttgc agctcgtgga
     2581 cgcgcgggtt gtcgctcgtg cgatcgacag ccgggagcat gcgtacgatc tgggtcaggc
     2641 ggatcagcac ataggtcagc ggattgttga tctcgtgcgc gagccccgcg gcaagcgtgc
     2701 cgagcgtcgc gagtcgatct acttcggctg ccttgaccgg tgcgagtcgc ttgtcccgct
     2761 cggcgatcac ctgctcgatc gccttgctca acatgtttcc atcgatcggc ttgcgcagaa
     2821 agagctcggc atcgactgcg gcggcggccg gattggagct gccggaaatc gccacgaccg
     2881 ggatcgtcgc gagcaccgga tccttgcgtt gcgcgagacg aaactgccag ccatcgagac
     2941 ctggcatcat gaggtccagc acgacaacgt ccgggcgagt tgcccgtaac tggcgcaggc
     3001 cttcctgacc gtcgacggcc gtcagcacca cgtgtcccga gtcggcgagc gtttcgcaca
     3061 gcgcatcgcg gaggtcggtg tcgtcctcga cgaccaacac ccggagcggt gattcggcgg
     3121 ggcgtgcgct agcgctcgct tgcatgcgtg cgctagcgat caagcgccgt gccggtgatt
     3181 gctttcgaga gcggcgagct ggcgcagctt ctcccacaaa tgcttccgcg agatcccaag
     3241 tagacgcgca gcctgcgtct tgttgccctg cgtcgcctcg agcgcgtgac gcaggtactc
     3301 gcgttcgaat gcgtgtagcg cgtcggcgag cggtttgatc tcacgttcgc gtgacggcgg
     3361 gatggccttg agggtcggaa caatctcccg tggcagatgc tcgagatcga tctcgcaacc
     3421 gccggcgagg acgatcgcac gttggatcgc gtgctcgagc tcgcgaacgt tgccggggaa
     3481 cgagtaggtc gacaccatcg cccaggcgcg cggcgagagc gtggggcgcc gacctggctg
     3541 cgcgtgcttt tgcagaaagt gctcgacgag caacgggaga tcgccggtgc gctcgcgaag
     3601 tggaggcacg tcgagatcga ggactttgat ccgatagtag agatcaccgc ggaacgatcc
     3661 atcggcgacc atcgccgaga gctcgcgatt cgtagccgag atcagccgaa cgtcgacaac
     3721 gatctcggca ttcgtgccga gcggctgaat ccggccgtcc tgcagcacgc gcagcagctt
     3781 ggcctgcgcc gcgagcgata ggccattgat ctcgtcgagg aacagcgttc cgccctgcgc
     3841 ggccttgaac ttgccgtcgc gcttctgcat cgctccggtg aacgcgccgc gctcgtgacc
     3901 gaatagctcc gcttcgatca gggtctcggg aaatgcggcg cagtttacga tcacgagagc
     3961 ttcgttgcgg cgcgagctcc gctcgtggat gagatgcgcg agcaactctt taccggtccc
     4021 gctctcaccg gtgaggagta cgggtgcgtt ggttactgcg atcgcggata gccgcgcgag
     4081 cacgagctcg atggccggag agcggccgat gatcggtagc gcgggatcga ggtcctcggt
     4141 aagcgcgcgc cgcgcgtgtg cgaacgtctc gtgggtcgtg gcacgttgct cgtgctggtc
     4201 gacgatcacg acgagatcgc gttcggtgac cgggggccgc aggcgcacca gctccaccgg
     4261 cgccgcaaca cggtcgcgca gctcgctcgc atctgatgtg gtggccgtga caacgaggtc
     4321 gaacgcggtt tccgttagga ggccgatcgc ctgcgacgag tcgacggcga ccacgtcatg
     4381 gccgtggccg cgcagcgtgg tggtcgccat gctgcgtgcc gtgccatcgt cgtcgataac
     4441 gaggatccgg gacattcgcc gctctgtttt caaggctagt cccggaattc actctgatca
     4501 ccctcgctgt aacgtggaac aacggttact ggagcggacc acgcagatcg ctaccaaatg
     4561 gtgacggtgt tcctttggat cgtgtcggtt gggtaacgcc gaccgctcgg ctcgattaca
     4621 cacatcgcag cacaagaacg gacgtgatgc gcgtgcgcgc gtggcacgca cgatgaacgc
     4681 cgcgcccgtc atgagggtcc tcgtgatcga tgatgacgac agcctacgcg cgatgatcag
     4741 cgagctgctg atcgagatcg gatacgagct tgtaggcttc gcgagtctcg acgaggttgt
     4801 ggacaatgcg agctttcgca tcggccgcga cgatctgatc ttgatcgatc tcgatgcgcg
     4861 acgagccgcc gagctccgcg cgccgctgac gagcgcattc ccatacacag tcgccatcac
     4921 cgtcggagac ggagactacg aagcgattcc tcgcgtgcag cttcgaaagc gtgcgaggcg
     4981 tgcgcgcgcg tcacagcgag cgagagcaac acgcgcgacc ggcgcaaatt agtcgcagcg
     5041 gtgaccgtcg cgcgtccgca cgcgaagtgg cacagacgtt gagttacgtc gcttcgcagt
     5101 caacggtcac gcgcagcgag ggcgcgtggc acctggaggt agcgatgaag tcactgcggc
     5161 gccaccgaca gtcttctccg cggcacgatg ccttggcttc gtcgagcggc acagccgagc
     5221 gcctggatcg gccaatccgc gacgtgatga gccgagactt catctgtgtc tctggcgacc
     5281 tcggcatcga cactcttgaa gagctgttgc tcgagcgtga tctgtcgggc gtgccggtca
     5341 tcgatcacga gcgccgcttg atcggctacg cgacgatgac cgacatcatg cgcgaacatc
     5401 acgatcgtcc ggatccggcg cctgcagagc gcacggaatg gggagggcac accgaatcgg
     5461 caccgaagac cgtcgccgag gtgatgacgc cggtcgcact cgagctacag gagcagagca
     5521 ccgttgctat cgcgatcgag ctgatgacgt cgcgcggggt gcatcgcgtg ccggttgtat
     5581 ccaatgaagg cgtattggtc ggcatgatca cggccgacgc gatcctgcgt tcgttcgcaa
     5641 agcgggccac caaggtgcat gtcgacgagc acacggccga ggccgatcgt gtcgtctcgc
     5701 ttggattcat gacgagtggc ctcgcccacc aggtcaacaa cgcgctcact ccgatgcgtc
     5761 tcagcctcgg ccgactcacg agcttcgagc tctcgcggcg gccgttatcc ccggagcgca
     5821 tgcatcgcgt cgagcttctt caggatgtcc gcgagggact cgagcgcatc gagcggatcg
     5881 ttcgtgcgct aaaggtgttc tcgcacgcgg atgagaagga gagtcatccg gtggatgtct
     5941 gcgacgcgct cgagaccgcg ctcgccgtcg cgaatcacga gatccggcat cgcgcgaagc
     6001 tcgtgcgcga cgtcaacccg atgccgctcg tcgacgcgcg tgccgaggac ttgcgccagg
     6061 tcttcatcaa cctgttgatc aacgcagttc acgcgatccc ggaaggcgag gcgcatgttc
     6121 acgagattcg cgtcacgacg tggacgaacg cgcgaggtcg cgccgcggtc gaaatcaagg
     6181 acagcggcac cgggattccg gtcgaggctc tgcctcgcat cttcgagccg ttcttcacga
     6241 cgcatcccgg tcgcgcactg ggcctcggcc tcgctgtcac gcgggacctc gtggcgtcgt
     6301 tcggtggcga gatcgaagtc gacagcgtcg tcggtagagg caccgcgatc cggatcacgc
     6361 tgccgccgag tgaaaagacc gctcagacat cgatcgcacc gcacgacgag cgtggccgcg
     6421 aggccgaggc cagaacgcgc ctgcggatcc tgatcgtcga cgacgatcgc ccggttgcgg
     6481 ctgcgatcgc gctcgagctt ggtgagcacg atgtcatcgt tgccgaaagc ggacgcgagg
     6541 cactcgagat tctccgccgc gacaaagact tcgacgtgat cctctgcgac ttgatgatgc
     6601 cggaagtcac cggcatggat gtctacgagt cgctgcggct gattgcacct gcactggttg
     6661 gccgcgtcgt gctcatgacg ggcggcgcgt tcacgcatcg agctggcgaa ttcttgtcga
     6721 cggtcaatgc gccggtactc gagaagccgt tcgagtcagg tcagctgcaa gcgatcgtca
     6781 atgcgatcga gcggcagcac gagctggccg aagcgccgat cctcgcggcg gactacgcgg
     6841 tgacaacgcc ggtgataggc ggaagggaga actagcgatg gccgagcaac acgaagatcc
     6901 cgaacgactt cccgatggcg taccgcacat cgttgtcggt ctcaccgccg cgtgtgccgt
     6961 cgtgctcgcg atcgtgctcg tgctggcttg gtcacacgaa cggatttcgg tcatcgtgct
     7021 ggcggcggtc gtcaccccgg tgctcgtgtc gaagctcgtc cagaaatccg aacgagaacg
     7081 cgatcacgtc cacccttcac gatgacggag gccgcccatg catcctcatc tttccactcg
     7141 gacgagatac acacgtcagg tcaagcgcga cgtcggcggt aacgcggcga ttcgcgcgcc
     7201 gcctcgacga ctgctctcgt tcaagctgag tacgtgcgcg tcatgcggcg catcgacgac
     7261 acgtccgttg ttttgcgtag actgtgagga ccgcgcgcgt ccacaccaag acgacgaccc
     7321 ttacgacgtt ctcggtggtg aaggcgagga ttaggatggc gacgcgcgcg gccctcacgc
     7381 tacgctctta tcgatgcttc acaatcgatc tgcgccgcac gcgcgtgtcg tgccggtgct
     7441 taccgtcgac aacgttcgtg cggcggtcac gtggtatgcg acggtgttcg gcttcgtcga
     7501 gcacgtgcag attggagaag gacatcgcgc tcaactcgga ctcccgggag gcggcgagct
     7561 gatcgttgcg gaagtgcgcc cgggccgccg aaccccgagc gcgggtcgat cgcaccaggt
     7621 catgctgaag gtcgaaaatg cggcggccgt cgtggcgctt gctcgcgaga acggagcgac
     7681 catcgtcggc gagccgcacg actgggagtt cggcgagcgc caggcgacga tcgacgacgt
     7741 attcggccat cagtgggtac tgacgcagac gctcgtcgac accgcgcccg agcagtgggg
     7801 cggcaagacc gtcactcccc gctgaaacac cccttcagcg tatcgatcga gctcgccgct
     7861 gcgaagcagg tccggagctc catcgaccac gagccgacgt cgcatgcagc ttcgacctgc
     7921 tgcggaagat ccgtgacgtc ttcgtgcatg ccggcggcct gaatcgtcgc cgcgatctgc
     7981 ggaccgagca ctgcgcacga caggttcgcc ggcaccgagg tcggcgcctg ctggggcacc
     8041 tgccccgcgc acagatgcgc gttgatcaga tcgccagcgg cgagcgtgca gttgcgctga
     8101 tcgaccgacc agtgctcgga ctcgcaattc gccgcgtagt gcgcgccaca cgtctcgaag
     8161 ctctcctcgt cgggctgtgc gttcggacca tgcgtcgtgt cggcttgcag ctcggccagg
     8221 tggcgcccga ctgccgcgca gtcgttgctc tccgccatcg agccgctctg gatcagcggc
     8281 gcgagtgacg gagcatgcgc ctgcacgcgt ttggcgacgt cgcagagcga tccgtcgtgg
     8341 ctgtgcgcgg cagttgcgcc cttcgcagcg tgctcgttgt cgagcgcggc gtgcgcggcg
     8401 gcggcatgcg gttcagcatg cgcgtcgctg tgcgtcgtgg tgaggacaag cgcagcagcg
     8461 cctccaagcg tagcggttac agcagcagca atagcaagtt tgtgcatcgt cgatcctttc
     8521 acagcgggtg aggcatcgac atgcgaagca gatgcggtga cgccgacgag cgcaagtacc
     8581 gacgcggcga atccgggccg cgggccgcgc cgcgcgagtc cacgctcgac cgtcgcgacg
     8641 cgttcggcga gatagcgccg accgcgcgac aaccgtttgt tcgtggttgc ggcgctgatt
     8701 ccgagcgagc gcgcgacgtc agcgatcgag cgttcctcgt agtaatagag gacgagcggc
     8761 tcgcgataga cgtcgggcac cagcgcgagt gccgctgcaa cggcgcgctc cgactccgcc
     8821 tcggtcatct gatcgtacgg cgtcgtcgcg tgggtcacgt catcgacgtc accgacggtc
     8881 tcgcgatgcg cttgccggcg catgtcgcgc gcgcggttgc gggcaatccc gcacaaccac
     8941 gctgcgagac gttgctcgtc gcgaagctcg gcaaggcgcc gccacgcgat cacgaacgtg
     9001 tcttgcgcga cgtcgtcggc aagcgctcga tcgcgcacgc ccgaaaacgc caccgcatac
     9061 accgcgcgct ggtagcgttc gatgatctga gcgaaggcgt cgcgatctcc gcgccggctc
     9121 gcctcgatca gcgtcccgtc acctgcttgc atgtcgtctt gtagtggatg tcggggccga
     9181 tttcggaccg aaaatccgca agcagaagac ctcaatgatt cagagcgcgt acgtcagcgc
     9241 gccacgcggt agatgtcgcc ggtgtccttg ttggcaaagt agaacgccgg tggcgttgct
     9301 acgaccgcaa atggatcctg cgaatcgagc agcgtcgtct cgggaccgcc ggtggtcgac
     9361 aagacatacg agccccactg caggctgtca tcgatcgcgt cgatgcccga gcgcaagcgg
     9421 tgcggagtca tccccaatgg ctcgacggcg ccgccggcac gagggacgcg atacgccgtc
     9481 gcgtacccgc acgtcccggt gcagccgacg aagtagacgt actgatcgtc gatggtcatc
     9541 tgcggcgcga tcatgttggt cacgagatcc gtgatctgtt tggtctgcac ttcgacttgt
     9601 acgacgccta gcgaatccgt gaagtagagg atgccggcat cgatgctcat ctcgtagggc
     9661 gtcgcgttcg cgcgtgcccg agcgatgaag tgcgggttgc cacccgtcgt cggcatcttc
     9721 aagatgcgcg cttcgccggt agtactagcg agcgtgtcgc cgtagtagat ccaaccgcca
     9781 tcgacaacca gtccgcgcac acccatcggc gtcgccacgg tcgtcggcgt accgccggtc
     9841 actggaaagc tcacgatcga tccgacatag ttgttcagcg cgtctcgagt aacggcgacg
     9901 tagacgaccc cgccgacgac ctggagtccg aggacccagc cgggcgccgt atagagcgtt
     9961 tcgatcgcac cgcctgcgag cggcatgcgt tggatcgcaa tggcggagct gtcattgcgc
    10021 gcccagtaga gatgcgtcgt gtcggtccgc aggatctcga tgccagggcc gccgttcccg
    10081 agcggctgca gcgtggtcgc cgcatcggat cctgagccac cgccgccgcc accgccgtca
    10141 ccactacctc ctcctcctcc tcctcctcct cctcctccgt ccccaccgtt cggatcgatc
    10201 gccgcatcgt tacgcggatt gcctccgccg ccggagccgt cgtcatcgtc gagtcccgac
    10261 gaggtcgaac cgcacgcgac gagccacagt ccacacgcga tagcccacaa ccgcatatcg
    10321 accatgttcc ccgattgcga cgttggttgc gagcacgatc gtcgcgccaa cggttagtgc
    10381 aacgaacggc tgcacttgcg caggcaacac gaagtcgtta gcgccgcgtc ggcgccgcga
    10441 ggtgcgtgcg cacgtcccac agctccgggt agaacgtgag ctcgagcgcc tgcttgagga
    10501 agccgacgcc ggagctgccg ccggtaccct gcgcaaaacc gatgatgcgc ttgaccgtcg
    10561 tcatgtgccg gaagcgccac agctggaacc gctcctccac gtcgacgagc ttctcgcaca
    10621 tatcgtacgc atcgtagtgg cgctcgggct ctgcatagat ggtgcggaac acctcgagca
    10681 ccgccgcgct ccgcgtgtac ggctgcgtga agtcacgctc gatccgatct tgcggaatcg
    10741 cgtagccctt gcgcgcgagc cagcgcagaa actcgtcgta gagcgacggc gcggcgagcc
    10801 gcgcagcgag ctcggcgtga atgtgcggat cgtgcgcgtg cggcttgagc gtgttcgcgt
    10861 ccttgttgcc gagcacgaac tcgagagcgc ggtattggta gctctgaaaa ccgctcgcca
    10921 cgccgagcgc atcgcggaac tggaggtact cgctcggcgt catcgtctcg agcacggccc
    10981 actgttcgaa cagcatccgc tgcacgtgag cgacgcgcgc gaagatcttg aacgtctcga
    11041 cgagccgatc ggagcggacg agcttcacgg cgagatcgag ctcgtgaagc agcagcttga
    11101 tccacagctc gctcgtgtga tgctggatga tgaacagcat ctcgtcgtga accgccgtga
    11161 ccggacgctg cgcggcgagg agctcgtcga gatgcagata gtcgccgtag gtgctgccct
    11221 tcgacagctt gaggtgcatg ttgctctcga gctcgcggcg gttctcgctc atggcgggaa
    11281 tctactccag actcgaggtt ctgcccgccc atcgaaacgc ggtctcgacc tcacgggcgg
    11341 actaaccacc cgagtatgtt gcgcaagatg cgcgtagtaa ttgtcctgtg tctggtggct
    11401 tgtggcgaag ctgcccgacg ctgggctgta acagtcactt gcgccgggac gctcgcgtgc
    11461 cttcgatcaa tgcgcgcttg gcgcgcagcg cgttcacgat gaagtcccgc agcacgcgca
    11521 cacgcgtcgt cgtgcgaaga tcgcgatgcg tgagtaccca gagcggtgca gacgcttcgt
    11581 tcatcagccg cacgcgccgc caggcgggca tcacttcgcc gagcgcgcac gggatgatac
    11641 tgacgccagc gccagcatcg atgccttcac gcagacccca gcccgtggtc acgcgcaccg
    11701 cgacacgagc ccgcggtacg ttcttctcga tccaccgtgc gaacgccatc tcggagcctg
    11761 cctcccagcc gacccagtcg agcgcttcga gatctttcgt gttgcgccca cgcagatacg
    11821 actcggagcc gtagatgccg acgccagcct tgcacacgcg atggccgacg agctcgggcg
    11881 gcggatcagc ggcgacgcgg atcgcgacgt cggccgcgcg atgcgcgaga tcgacgaagc
    11941 cggtaccgag tgcgaccgtg acgtcgatac cgggctgcgc ctttgcgagc tccgcaaaga
    12001 tcgggaacaa cagcggcgcg aacggatcgg ggacggtgac acgcaccggg ccggtcaacc
    12061 gcagatcctg tccgctcaca cgacgctcga gattcgtcac cgtttcttcg agagtcgtcg
    12121 cggtttcgaa cacgtcctgt ccggcgggcg tggcttcgta gccttcaccc ttgtcgacga
    12181 acagctgcac gccaacggcg gtctcgagcg agtgcagacg ccggagcacc gtcgagtgac
    12241 tcacgccgag ctcgcgtgcc gctttgcgga tcgaaccggc gcgaccgagc gacagcgcgg
    12301 tgcggagatc gtcccaatcg atgtggtgca gattcggacc agccctactc atttttgcag
    12361 tctatcggtc cgcaagcggt ccgggtacca aggaggcatg caaacgcact cgatcgctaa
    12421 cgcccgcacg tcgaccgagg tgatggtcct cggacctggt cagggcgagg acgtctggtt
    12481 cacggacaac ctgctcacga tcaaggcgcg tcccgcgact ggcacccagt tcggcgtcat
    12541 cgagagcacg ctgccggaag gctcgcatac gccgtttcac cgccacgacc acgaggacga
    12601 agcgttctac gtgctcgaag gccggatgaa gatctacgtc gatggccgcg cgcccatcga
    12661 agtcggtccg ggttgttacg tgcacacgcc acgcggtgcg gcgcacggat tcgtgacgct
    12721 gacgccggtg cgcatgctcg tgctgtgcgg cgtcgaaggc ttcgtcgaga tggcgcgcga
    12781 agccggcatg ccggcgcctc gccacgagct gccgcccgcg gggccgcccg atatcgcacg
    12841 gctcgaggcg gcgtgcgcac gaaatcacat cacgttgctc ggcccgctgc cggggtgatc
    12901 accggagtcg cgcgcggata tcggcgtacg cggtgtcgac gagcttggcg agcgccgcag
    12961 catcgattgg ctgacccggc cgcagcttca cgtgccgccc gcgcttaccg gttccttcga
    13021 gcaggcgagc cggatcgtcg agcagcgcgc cgaagaagaa gccgacgttc acgtgatctc
    13081 ggaacgcgtt gacgtagccg aatgccgcct cgccgacaca cgcggtcggc attccgtcgt
    13141 gcatgagctc gcgcacgtcg ctgccgcact gccgcatgcg cgcgaaccag gtctcgacga
    13201 acggccgcag gtcatcgcgt tgcgcacgta accaggcatc gattgctggc tcgtgtttca
    13261 ccgccgtcgg gaagcgcatg atgccgtcca cgccgtgatg atactgcacg tggcgcgaga
    13321 cgattgcggc ggcgcgcgca tccaagcgtg gtgacgcgat tcgctgcgat gtcgtggctc
    13381 accagcgcgg tgacggttgt cgccgcactc gggacggcgg tcctccgcgc gatcggtagc
    13441 gagtcgtggc ggccggcgct cgcggtgact ctcgcggtct tcggcgtgcg cgtggctgtg
    13501 atggcatggg gtgcgtcgcg ccgcgagctg ccatggtcgc gactgctgtt gccgggaatc
    13561 atctggctcg agggcgcggg cctggcgacg agctcggcgg ccacgctatg gcaggtgcgg
    13621 ctcgggactg cggtcatgct cgagctcgga ttcgtggtgg tggcgattcg cgaggttcgc
    13681 cgcgtctcgg cgagcaacga gccggtggac gaccgacttg cacgcgcgtt cggtgcactg
    13741 ctgccgccgg gcgcagcgcg cttcgtcgcg ttcgagctcg tcatcattgg atcagcgctg
    13801 cgcttcgtga tcggcggttg gcgccgcgcg gtacccgcgg gcttcaccta tcaccgcgag
    13861 agcgtgctgc gattgttcct gcagttcctg ccgatcctcg gagtcgcaga tctactcctc
    13921 gtcgagctcg tgctccttcc gcacgcggcg acgtggctcc ggctcgtcgt gcacgggctg
    13981 gcgatctacg gcctggtctg gttggtcggc ttctacgcct cgctgcgcgg ccggccgcac
    14041 cagctcgcga atggccggct catccttcat cgcgggatgc tgagacggtc gccgtgcccg
    14101 tgaccgacat cgcatccgtc gaggaattgc ccgtgttcgc agacgattgg aagaagcgcg
    14161 cgtatcgcaa aggggcaatc cggatcgacg tgccgggccc gaccgtcctc gagcttcgtc
    14221 tgcgtacgtc tgcccggccg ttcggtgtgt tcggcaacgg cgccgcgagc acacgtgtgc
    14281 tcgtgtcggt cgacgagccg gcgtcattta tcgctgcagt ctcgggtacc gcggaacccg
    14341 cacacgacta gggtgtattc cggtgcgttc gtgcggctcc aggcgttgat cgcgccgcgt
    14401 acatcgcgtg tgatcgagtg atgaagcttc cgcggccctc agcgcagttg accagcacgt
    14461 tgaccgagct ctccgccatc ttgcgccagc cggccgtgtg gactccgcac gcgcggctcg
    14521 tcggcaggaa gctgccgatg atcgtcaagg gcctgcgcat gatcggcaag accgacaaga
    14581 ccgaggcgct gtcgatcggc tcgttgatcg aacagaacgc gaagcttcgt ccgaatgccg
    14641 cggcgattct ctatgaagac cgtcgctatt cagcgcgcga cgttcacgag atgagcaatc
    14701 gctgggcgaa ctggctcgcg gcgcgtggcg tcggcaaagg cgatgtcatc tcggtgctgc
    14761 tcgagaatcg tcccgaggca ttgctgatca tcggcggcat cgtcaagctc ggcgcgatcg
    14821 ctgcggtgat caacacgaag cagcgcggcc ggacgctgca acacagcctc gcggtggcga
    14881 aggcgcggtt gcatctcgtc ggcgaggagc tgtgggattc cttcgtcgag gtccgtgacg
    14941 cgatcggcgc gagtgacaac gtcgcatggg tgcgcgagca aaccagtgcg cgcgttcccg
    15001 ctggcgcgat cgacgcggcc gcagccgttg ctaacgcgtc gctcacgacg cctgccgcgc
    15061 tcgccgacgt acgtctcggc gacccagcgt tctacatcta tacgtcgggt acgacgggcc
    15121 tgccgaaggc atcgatcatg tcgcacaacc gctggaccaa agcggccggc gcattcggca
    15181 tgtcggcgct cgcggtcacc gaagccgacg tcatctacct cgcgctgccg ctctatcaca
    15241 acaacgcgct caccgtggcg tgggcgtcgg cggtctcggg cggcgctgcg ctggcgatcc
    15301 gccgcaagtt cagcgtcagc aatttctggg aggacatccg caagttccgc gcgaccgcgt
    15361 tcatctatat cggcgagctg tgccgctact tgctcaacgc ggaaccgcgg cccgacgatc
    15421 gcaaacattc ggtccgcgcg atctgtggca acggtctgcg ccccgacatc tggaaggcgt
    15481 tcaagacgcg cttcggcatc gacgaggtct acgagttcta cgccgcatcc gaaggaaaca
    15541 tcgcgttcgt caatctactg aacctcgacg cgacggtcgg tctgtgccct gcgccgtacg
    15601 cactcgtcaa atacgacatc gatcgcgacg aaccgctgcg cggtcccgac ggtcacatgg
    15661 tgcgcgtcgg tcgcggcgag atcggtctac tgatcggcga ggtcaccgag cgctacgcgt
    15721 tcgacggcta caccgacaag gccgcgagcg agaagaagct gttccgcgac gtcttccgca
    15781 aaggcgacgt ctggttcaac tcgggcgatc tgcttcgcga ccaaggcttt cgccacgcgc
    15841 agttcatcga ccgcgtcggt gacacgttcc ggtggaaggg cgagaacgtc tcgacgaacg
    15901 aagtcgccga ggctctcaac acgttccacc aggtcgccga gtcgaccgtg tacggcgtcc
    15961 aggttcccgg taccgacggc aggtgtggca tggcagcgct cgtccttcgc tgtgccgtca
    16021 acgacttcga cttgcgcgga ttcacgcgtc acgtcgagtc gcagctaccc gcgtatgcac
    16081 gtccgctgtt tctccgagtt cgccccgagc tcgaagtgac cggtacgttc aagcaagtga
    16141 agagcgatct gcgtcgccag ggtt
//
LOCUS       DASUFX010000656         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_659559,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000656 DASUFX010000000
VERSION     DASUFX010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659559"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /gene="metH"
                     /locus_tag="VFV99_26665"
     CDS             complement(<1..2186)
                     /gene="metH"
                     /locus_tag="VFV99_26665"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5062997.1"
                     /translation="MSAVDDLRELLERRIVVLDGAMGTMVQGLGLADEAVWRGDRFHN
                     HPVSVKGCNDLLALTKPELIEEIHLQFLRAGADIVETDTFTATSIALADYGLEGVVHD
                     LNFAAAQAARRACARATKEDGKPRWVAGSIGPTTKSASLSPDVNDPGARSVTFRELVA
                     TFGEQARALIEGGVDLLLTETHIDTLNCKAALYAFEELFAQGVRRVPVIASVTIPDKS
                     GRTLSGQTVEAFFNSISHANLFAVSINCALGADDMRPHVAELARIAGVRVACYPNAGL
                     PNEFGEYDDTPEHMAKVLGGFARDGLLNIVGGCCGTRPEHIKAIRAAVDGVKTRTIAK
                     PPHHMRLSGLEPVTITPETNFVVIGERTNVTGSAVFRRLIKEDKYDEAVAVARQQVEG
                     GANIIDVCMDEGMLDGVAAMKRFLNLIASEPDIAKLPVMVDSSKFSVIEAGLECLQGK
                     GVVNSISLKEGEAEFVRQATIVRKFGAAVVVMAFDETGQATTVEHRVTIAKRAYKILT
                     EQVGFPPEDIIFDPNILTIGTGMEEHDDYAVGFIEATRRIKTELPGTKVSGGVSNLSF
                     SFRTSPRVREAIHSVFLYHAIKAGLDMAIVNAGQLAVYEQLDPQLREAVEDLVLNRRP
                     DATERMLALAATFGGAAEKKEDDLAWRKQPLKDRLAHALVNGITDFIDPDVAEALTVY
                     AKPLDIIEGPLMDGMNIVGELFGSGKMVLPQVVKSARVMKKAVAIL"
     gene            2309..>3059
                     /locus_tag="VFV99_26670"
     CDS             2309..>3059
                     /locus_tag="VFV99_26670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5062998.1"
                     /translation="MEFTGRQLGRYHLLRLLGFGGMGEVYLASSRGAAGFEKLVTVKI
                     LRKQVSTNPERVKDLLREAFIGVRLDHENIVQVLDLGEHEGTYFLVMEYVRGFSLREV
                     IAFHSEQRETLPIRPIAHAIRCMADALDYVHRVRGARRKHIGLIHRDVSPSNILLGAE
                     GRIKLSDFGVAGMAQDAARAGTVIGKPRYIPPEARFGECATQGWDIYALGVVLHSALA
                     ADRDIERGTTQIGERLKPLLELRPDCPPGLVG"
BASE COUNT          505 a         1004 c          993 g          557 t
ORIGIN      
        1 tcgaggatcg cgacggcctt cttcatcacg cgggcagact tcacgacctg cgggaggacc
       61 atcttgccgg agccgaacag ctcgccgacg atgttcatgc cgtccatcag tgggccctcg
      121 atgatatcga gcggcttcgc atagacggtc agcgcctcgg cgacatccgg atcgatgaag
      181 tcggtgatgc cgttgacgag cgcgtgcgcg agccggtcct tgagcggctg cttgcgccac
      241 gcgagatcgt cttccttctt ctcggcggcg ccaccgaagg tcgcggcaag cgcgagcatc
      301 cgctccgtcg catccggccg gcgatttagc accagatcct cgaccgcttc gcgcagctgc
      361 ggatcgagct gctcgtacac cgcgagctga ccggcgttga cgatcgccat gtcgaggccc
      421 gctttgatcg cgtgatagag gaacaccgag tgaatcgcct cgcgcacgcg cggcgacgtg
      481 cggaacgaga acgacaggtt cgagacgcca cccgagacct tcgtgccggg cagctcggtc
      541 ttgatgcgac gcgtggcctc gatgaagccg acggcgtagt cgtcgtgctc ctccatgcca
      601 gtgccgatcg tcaggatgtt cggatcgaag atgatgtctt cgggcgggaa gccgacttgc
      661 tcggtgagga tcttgtaggc gcgcttcgcg atcgtgacgc gatgctcgac cgtcgtcgct
      721 tggccggtct cgtcgaacgc catcacgacc accgctgcgc cgaacttgcg aacgatcgtc
      781 gcctggcgca cgaactcggc ttcgccttcc ttgagcgaga tcgagttgac gacacccttg
      841 ccctgcaggc attccaggcc ggcctcgatc accgagaact tcgacgagtc gaccatcacc
      901 ggcagcttcg cgatgtcggg ctcgctggcg atcaggttca agaaccgctt catcgccgcg
      961 acgccgtcga gcatgccttc gtccatgcag acgtcgatga tgttcgcgcc gccttcgact
     1021 tgctgccgcg cgactgcaac cgcttcgtcg tacttgtctt ctttgatcag gcggcggaac
     1081 accgccgagc ctgtcacgtt cgtgcgctcg ccgatcacga cgaagttcgt ctccggcgtg
     1141 atggtcaccg gctcgaggcc cgacagccgc atgtggtgtg gaggcttcgc gatcgttcgc
     1201 gtcttgacgc cgtcgaccgc ggcgcggatc gccttgatgt gctcggggcg cgttccgcag
     1261 caaccgccga cgatgttgag cagaccgtcg cgcgcgaacc cgccgagcac cttcgccatg
     1321 tgctccggcg tgtcgtcgta ctcgccgaac tcgttcggca ggccggcgtt cggatagcag
     1381 gcgacgcgaa caccggcgat gcgcgcgagc tcggcgacgt gcgggcgcat gtcatcggcg
     1441 ccgagcgcgc agttgatgct caccgcgaac aggttcgcgt ggctgatcga gttgaagaac
     1501 gcctcgaccg tctggcccga caacgtgcgg ccggacttgt ccggaatcgt caccgacgcg
     1561 atcaccggca cgcggcggac gccttgtgcg aacagctctt cgaacgcgta gagcgccgcc
     1621 ttgcagttga gcgtgtcgat gtgggtttcg gtgagcagca ggtcgacgcc accttcgatc
     1681 agcgcgcgcg cctgctcgcc gaacgtcgcg acgagctcgc ggaacgtgac ggaccgcgca
     1741 cccggatcgt tgacgtcggg agatagggac gcggacttcg tggtcggccc gatcgagcca
     1801 gcgacccagc gcggcttacc gtcttccttg gtcgcgcgag cgcacgcgcg gcgagcggcc
     1861 tgtgccgccg caaaattgag gtcgtgcacg acgccttcga ggccgtaatc ggcgagcgcg
     1921 atcgacgtcg cggtgaacgt gtcggtctcg acgatgtcgg cgccagcccg tagaaactgc
     1981 agatgaattt cttcgatcag ctcgggcttc gtgagcgcga ggagatcgtt acagcctttt
     2041 accgagacgg gatggttatg gaagcggtcg ccgcgccaca cggcttcatc ggcgagcccg
     2101 agcccctgga ccatggtgcc catcgcaccg tcgagaacga cgatgcggcg ttcgagcaac
     2161 tcgcgcaggt cgtcgacggc agacatccgc ccctgattac cactgtttcg tgaactcacc
     2221 taggggcggc cacacgtcgt cacgcaaaca ctgcagcgga tctacgcagg aaatcgtcaa
     2281 ttccgcgatg gtagactgag gctctagggt ggagttcact ggtcgtcagc tcggccgcta
     2341 ccacttactg cggctcctcg gattcggcgg aatgggcgag gtctatctcg ccagctcccg
     2401 cggtgccgcg gggttcgaga agctcgttac cgtcaagatc ctgcgcaagc aggtcagcac
     2461 gaaccccgag cgcgtgaagg acctcttgcg tgaggcgttc atcggcgtgc gcctcgatca
     2521 cgagaacatc gtgcaggtgc tcgatctcgg cgagcacgaa ggtacgtact tcctcgtcat
     2581 ggagtacgtg cgcggcttct cgctgcgtga ggtgatcgcg tttcactccg agcagcgcga
     2641 gacgctgccg atccgtccga tcgctcacgc aatccgctgc atggcggatg cactcgacta
     2701 cgtccaccgc gtgcgcggcg cgcgccgcaa gcacatcggc ctcattcatc gcgacgtcag
     2761 cccgtcgaat atcctgctcg gcgccgaggg tcgcatcaag ctgtcggact tcggcgtcgc
     2821 cggtatggcg caggacgcag cgcgcgccgg caccgtgatc ggcaagccgc gctacatccc
     2881 acccgaggcg cgctttggtg aatgtgcgac gcagggctgg gacatctacg cgcttggtgt
     2941 cgtgctgcac agcgcgctcg ccgccgatcg cgacatcgag cggggcacca cccagatcgg
     3001 tgagcggctg aagcctctgc tcgagctgcg gcccgattgt ccgcccggcc tcgtcgggc
//
LOCUS       DASUFX010000657         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1077203, whole genome shotgun sequence.
ACCESSION   DASUFX010000657 DASUFX010000000
VERSION     DASUFX010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077203"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1306)
                     /locus_tag="VFV99_26675"
     CDS             complement(377..1306)
                     /locus_tag="VFV99_26675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5062999.1"
                     /translation="MGLTTIVAGVDLSVPSEQALERAAALAQHNGATLVMVHAQADDA
                     PIEHADNAVLEQLGEVSAAVRAEEAKRLAEKLQQLQARGVSVELMSRTGAPGEVISEI
                     AKERSADLIVIGTHGHTGLQRFLLGSVATAVLRHSPCDVLVCRGVSGPSPFLRPLVAT
                     DFSPAAARALRNAAMLSGVNVPIEIVHAWQLPAGSWGATLLGQARFPWSTVRDAVLAS
                     AQAQADKLLAGNADIGHPVHLQLVQGPPQSVITNAAEQGAHDLIAIGTHGHRGFRRLL
                     LGSVAESVIRHAPCSVLVAHGEHAGETHKFKNA"
     gene            complement(1297..>2141)
                     /locus_tag="VFV99_26680"
     CDS             complement(1297..>2141)
                     /locus_tag="VFV99_26680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063000.1"
                     /translation="QSKALEEYEAEMRREEVEREAKTKEKLAKLDQLFGQKPAQMGTL
                     LEGIRLGSGAGSFQPEDVRQRIENATRDGFMLVSFDADAKQLNAVEVSLYGDYETGDV
                     CEKLDDKLTAAWGHPTNDAWLDTATHQRATLDKDDCKLRFDMYVEPADWVAQLPLAAV
                     GSSAEKLSQQLGGSGSVESDDDRLYWNVPGLRFGKGDTKIEAYAVNGKIVGFKVTASS
                     DFDSTLAVRDAISAKLKKQPKSEDSEYESSFHLYQWKTRVPVSLETSDSDRFTVMVGK
                     MPWD"
BASE COUNT          333 a          735 c          692 g          381 t
ORIGIN      
        1 gtgcggacgc tataccgcta cctgtcgtag gcctaacgtc agcgaagctc gacgcgtcga
       61 cgacggccga cgcgcagctg agcgagcgcg ttcccagatg gaacgccaag ctgccaaggt
      121 cgccaagaac gccaaggaaa aatctatccg gacaggataa cgagctgaga agcggtcacc
      181 gatccgggac gcccggtgcg cggttgatcc gccagccatg tgcccggtct gaaccgctct
      241 ccttgaacgt ttcttacctt ggcgttcttg gcgttcttgg caccttggcg ttgatccgga
      301 cggcgggttt ccgacgctcg accaaggacg gagcccgagc gacgcagcga accaacccgt
      361 cagattaggc gaagtactag gcgttcttga acttgtgcgt ctcgccggcg tgctcgccgt
      421 gcgcgacgag caccgagcac ggcgcgtgac ggatcacgct ctccgcgacg ctgccgagca
      481 gcaggcggcg gaagccgcga tgcccgtgcg tgccgatcgc gatcagatcg tgcgcgcctt
      541 gctccgcggc attcgtgatc accgattgcg gcgggccctg cacgagctgg agatggaccg
      601 gatggccgat gtcggcgttg ccagccagca gcttgtcggc ttgcgcctgc gcgctcgcga
      661 gcacggcgtc gcggaccgtc gaccacggga accgcgcctg gccgagtagc gtcgcgcccc
      721 acgacccggc gggcagctgc cacgcatgca cgatctcgat cggcacgttc acgccggaca
      781 acatcgccgc attgcgcagc gcgcgcgctg ccgcaggcga gaagtccgtc gcgaccagcg
      841 gccgcaagaa cgggctcggc cccgagacgc cgcggcagac gagcacatcg cacggcgaat
      901 gccgcagcac tgccgttgcg acgctgccga gcagaaaccg ctgcaggccg gtgtggccgt
      961 gcgtgccgat cacgatcagg tccgcgctgc gctccttcgc gatctccgag atgacctcgc
     1021 ccggcgcgcc ggtacggctc atcagctcga cggagacgcc acgcgcctgc agctgctgca
     1081 gcttctcggc gagtcgtttc gcttcctcgg cgcgaaccgc cgcagacact tcaccgagct
     1141 gctcgagcac cgcattgtcg gcgtgctcga tcggtgcgtc gtcggcttgc gcgtgcacca
     1201 tcacgagcgt cgcgccgttg tgttgcgcga gcgctgctgc gcgctcgaga gcttgctcgc
     1261 tggggaccga gaggtcgacg ccagcgacga tggttgtcag tcccacggca tcttcccgac
     1321 catgaccgtg aaccgatcgc tgtcggacgt ttcgagactc accggcacgc gcgtcttcca
     1381 ctgatagagg tggaagctcg actcgtactc gctgtcctca gacttcggct gcttcttcag
     1441 cttggccgag atcgcatcgc gaaccgcaag cgtggagtcg aagtcgctcg acgcggtcac
     1501 cttgaagccg acgatcttgc cgttcacggc gtacgcctcg atcttcgtgt cgcccttgcc
     1561 gaagcgcagg ccgggcacgt tccagtacag gcgatcatca tcggactcca cgctgccgct
     1621 gccaccgagc tgctgcgaca gcttctcggc ggaggaaccg actgccgcga gcgggagctg
     1681 tgcgacccag tctgccggct cgacgtacat gtcgaagcgg agcttgcagt cgtccttgtc
     1741 gagcgtcgcg cgctggtgcg tcgcggtgtc gagccatgcg tcgttcgtgg gatgccccca
     1801 cgcggcggtg agcttgtcgt cgagcttctc gcagacgtcg ccggtctcgt agtcgccgta
     1861 gagcgagacc tcgaccgcgt tgagctgctt cgcgtccgcg tcgaagctca cgagcatgaa
     1921 gccatcgcgc gttgcgttct cgatgcgttg gcgcacgtcc tcgggctgga agctgcccgc
     1981 tcccgagcca aggcgaatgc cctcgagcag cgtgcccatc tgcgcgggct tctggccgaa
     2041 cagctggtcg agcttcgcga gcttctcctt cgtcttcgcc tcgcgctcaa cctcttcgcg
     2101 gcgcatctcg gcctcgtact cttcgagcgc cttgctctgc t
//
LOCUS       DASUFX010000658         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1910340, whole genome shotgun sequence.
ACCESSION   DASUFX010000658 DASUFX010000000
VERSION     DASUFX010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910340"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VFV99_26685"
     CDS             complement(<1..1335)
                     /locus_tag="VFV99_26685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063001.1"
                     /translation="MENGYADKTTQLALRAEAAQSNRHYLLVFERDLSTRVFQLPANG
                     TVIIGRGDDTALQLRDSSVSRRHARITQVDGRAQLADLESQNGTLINHDRVNEPQPLV
                     SGDQISLGGVTLVYHSSVTREVSPRSDDKARTISLGGRNVVVADPAMARLYELVERLA
                     VSELPVLVCGETGAGKDVVASALHQLSHRNGQPLISLNCAAIQENLVESELFGYERGA
                     FSGAVTAKPGLLETANGGTVFLDEIGDLPLAVQAKLLRVLETKRVLRLGDVRERAIDI
                     RLIAATHRDLGDDVVSGRFRRDLFFRLSGATLWIPPLRDRPRELPLLADMFLRDACRQ
                     QRRAAMQLSDATRAQLLAMPWPGNVRELKHLMEYLAAALHDNVIEPHHLEQPARLERM
                     STEIEVPQAPVKFRPLADEIRELEQSRITAALSAAAGNQRVAAQLLAMPLRTF"
     gene            complement(1516..1743)
                     /locus_tag="VFV99_26690"
     CDS             complement(1516..1743)
                     /locus_tag="VFV99_26690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063002.1"
                     /translation="MQTAIIELSKRAAPKRSPFGEFLVKHAVLDRFQLFRVLQMQDRI
                     PGTPLGACAVALGYAPRDQIEKLHVQYARSA"
     gene            complement(1789..2343)
                     /locus_tag="VFV99_26695"
     CDS             complement(1789..2343)
                     /locus_tag="VFV99_26695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063003.1"
                     /translation="MAGLARALLIAIIAMPSAASHAANDVAPAKQPQLGCEVLDHGIY
                     VPVTHRTRYSDVGSVTGERFEISEVRFVRHTKIIEPTLVQRFCIRYRLTGIASPSARI
                     TWRVVYPTPVRDMSSWQYSFRSSPTNGELVGHLLYDFVIASELVEGRWRFEVLVDGHA
                     ACSFGFVVQQPRTASIPAVLRSAT"
     gene            complement(2374..2601)
                     /locus_tag="VFV99_26700"
     CDS             complement(2374..2601)
                     /locus_tag="VFV99_26700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063004.1"
                     /translation="MLSVTSLLLHSEDVPETARLALANALQGPTEQRCENLFQAATIL
                     HREVGLDCGDARELVGLDDCGCDEARQELIS"
     gene            2711..3052
                     /locus_tag="VFV99_26705"
     CDS             2711..3052
                     /locus_tag="VFV99_26705"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5063005.1"
                     /translation="MPNADCQCRAFQAAVDVLGRPWNAFLLTMLQEGPLRFSEIAARP
                     NGPGDKVLAARLKDLEARGLIDRIVDAGPPIKVSYALTKQGRAFGDVSAAIQRWGRDL
                     KAPAKATRRAR"
     gene            complement(3049..3660)
                     /locus_tag="VFV99_26710"
     CDS             complement(3049..3660)
                     /locus_tag="VFV99_26710"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063006.1"
                     /translation="MDHEFWRARWNEGRTAFHEGKANAFLARHGEWLAQCHRILVPLC
                     GKTEDLAYLASRGHEVIGVELVEDAVRQFFAEHAATPEISERDGLEIYRAGVITIIAG
                     DFFATTRELVGTIDGIYDRAAIVALPPDIRSRYIAHLRAITPMAKRELLVSIDYPPGF
                     AEGPPFSVDGDEVRRWFPSAQLVDEGNDQRGRAERCYTIALAR"
     gene            3765..4670
                     /locus_tag="VFV99_26715"
     CDS             3765..4670
                     /locus_tag="VFV99_26715"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HEX5063007.1"
                     /translation="MSITYSKSIIAAIVLAVAATGNAQPTKDETQARSQCCVQKTSSV
                     QQSQVEARPTPPRAANPPKIDPQAIAALEKMGCFLRKQQNFTVSADAQTDYVIQNDLL
                     VTLPKHAELRVKRPNGLRIDVTSDRKQRQFFYDGKTFTMYGEKVGYYTSVPAPPTIHE
                     LANMLEDRYGLQLPLVDLFRWGTAQSPVKEITAAVRVGTERIDGVMTDHYAFRQKGVD
                     WQIWIEQGNRPVPHRLILTTTDDPARPAYALDMQWTLNTLNDWPVFTFVAPPDAVRIP
                     LSERTIQTVARAEQAKKRSSEPTKQ"
     gene            4717..>5166
                     /locus_tag="VFV99_26720"
     CDS             4717..>5166
                     /locus_tag="VFV99_26720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063008.1"
                     /translation="MLALGLVVIGAGLRIDDADARGGRGGGGRAGGMSRGGGGGFSRG
                     GGGMSRGGGARTSLSSSPRGGASRGSYGGGASRGSYGGGASRGSYGGASRGSYAGGGT
                     ASRGGAGAYGSPGYQRGGGAAAQRGAAGAGATRPSGGMAQGGTARPQA"
BASE COUNT          931 a         1628 c         1744 g          863 t
ORIGIN      
        1 gaacgtgcgc agcggcatcg cgagaagctg cgcagcgacg cgctggttgc cggccgccgc
       61 agacagcgct gcggtgatgc gcgactgctc gagctcacgg atctcgtcgg cgagcggacg
      121 gaacttcacg ggtgcttgcg gtacctcgat ctcggtcgac atccgttcga ggcgcgcagg
      181 ttgctcgagg tgatgcggct cgatcacgtt gtcgtggagc gcggcagcga gatactccat
      241 cagatgcttg agctcgcgca cgttgccggg ccatggcatc gcgagcagtt gcgcgcgcgt
      301 cgcgtcggat agctgcatcg cggcgcggcg ctgctggcgg catgcgtcgc gcaagaacat
      361 gtcggcgagc agcggcagct cgcgcggccg atcgcgtagc ggtgggatcc aaagcgtcgc
      421 gccgctgagc cggaagaaca gatcgcggcg gaatcggccg ctcacgacgt cgtcgccgag
      481 atcgcgatgc gtcgcggcga tcagtcggat gtcgatcgcg cgttcgcgga catcgccgag
      541 gcgcagcacc cgcttcgtct cgaggacgcg cagcagcttc gcctggaccg cgagcggaag
      601 gtcgccgatc tcgtcgagga acacggtgcc gccgttcgcg gtctcgagca accccggctt
      661 ggctgtgacc gcaccggaga atgcgccacg ctcgtaaccg aacagctcgc tctcgaccag
      721 gttctcctgg atcgcagcgc aattgagact gatgagcggc tggccgttgc gatgcgatag
      781 ctgatggagt gccgatgcaa cgacgtcctt gccggcaccc gtctcgccgc acacgagcac
      841 cggcagctcg gagaccgcga gacgttcgac gagctcgtag agccgggcca tcgccggatc
      901 ggcgacgacg acattgcgtc cgccgagcga gatggtgcgc gccttgtcat cgctgcgcgg
      961 cgacacttcg cgggtgacgc tcgagtgata gacgagcgtg acgccgccga gcgagatctg
     1021 gtcgcccgag acgagcggct gcggctcgtt cacgcgatcg tggttgatca gcgtgccgtt
     1081 ctggctttcc agatcggcga gctgcgcgcg accgtcgacc tgggtgatgc gtgcgtggcg
     1141 gcgtgagacc gagctgtcgc gcagctgaag agccgtgtcg tcgccgcggc cgataatcac
     1201 cgtgccgttg gcgggcaact ggaacacccg cgtcgagagg tctcgctcga acacgagcaa
     1261 gtagtgccga ttgctctgcg cagcttctgc tctaagtgcc agctgcgttg ttttgtctgc
     1321 gtatccgttc tccacgacct accacctaca gagaaggaac gaaccttccc acgcagccgg
     1381 cgcgatccgc aagctgaagt tttcgtccat gagatcgcgt atgcggcgac agcaccgcag
     1441 tcagcggttg tcagtgcagc cgttgcgcgg ctgatcgcgg ctcacgtgcg acgaattcgt
     1501 cgcgaatgcg gagcgctacg cactgcgcgc gtactgaacg tgcagcttct cgatctgatc
     1561 acgcggtgcg taaccaagcg ccacggcgca agcacccaac ggggtacccg gaatccggtc
     1621 ctgcatctgc agcacgcgaa acagctggaa gcgatcgagg acggcatgct tgacgaggaa
     1681 ttcgccgaac ggcgagcgct tcggtgctgc gcgcttggac agctcgatga tcgcggtctg
     1741 catctctaag gcagagcgag cgaagcaaat tcggtcgcag aaaattcgct aagtcgcaga
     1801 tctcaggacg gcggggatgc tggccgtccg cggctgctgg accacgaagc caaacgaaca
     1861 ggccgcatgg ccatcgacga gcacctcgaa ccgccagcgt ccctcgacaa gctccgatgc
     1921 gatcacgaag tcgtagagca agtggccgac gagctcgccg ttcgtgggcg acgagcgaaa
     1981 gctgtattgc cagctgctca tgtcgcgcac cggcgtcgga tagacgacgc gccacgtgat
     2041 gcgcgccgac ggcgacgcaa tgccggtgag ccggtagcga atgcagaagc gttggacgag
     2101 cgtcggttcg atgatcttcg tgtggcggac gaagcgcacc tcgctgatct cgaagcgctc
     2161 gccggtgacg ctaccgacat cgctgtagcg ggtgcggtgc gtgacgggca cgtagatccc
     2221 gtgatcgagg acctcgcagc cgagctgcgg ttgcttcgcg ggtgccacgt cgttcgcggc
     2281 atgcgacgcg gcggacggca ttgcgatgat cgcgatcaac agcgcccgcg cgagtcccgc
     2341 catgcgatcg agcgtactac gcggcgccgg cgatcagctg atcaattctt ggcgcgcctc
     2401 gtcgcagccg cagtcgtcca gcccgacgag ctcgcgggca tcgccgcagt cgaggccgac
     2461 ctcacggtgg aggatggtcg ccgcctggaa gaggttctcg cagcgctgct cggtgggccc
     2521 ttgcagcgca ttggcgagcg ccaaccgcgc agtttcgggg acatcctcgc tgtgcagtaa
     2581 caggctcgtt acgctcaaca tggactcaaa tgtgaggcac ggctttccat tgtcaaggca
     2641 ctttcttttg gaaagtgata gcgagaactt cccactttcc ttttgaaagt gccgttacta
     2701 tgttggcgac atgccgaacg cagactgtca atgccgcgcc ttccaggccg ccgtcgacgt
     2761 gctcggccga ccctggaatg cgttcctgct gacaatgttg caagaaggcc cgctgcggtt
     2821 cagcgagatc gcggcgcggc cgaatggtcc cggtgacaag gtgctcgccg ctcgcctcaa
     2881 ggatctcgag gcgcgcggcc tgatcgatcg catcgtcgat gctgggccac cgatcaaggt
     2941 cagttatgcg ttgacgaagc aaggtcgcgc attcggtgac gtgtcggcag cgatccaacg
     3001 ctggggacgc gatttgaagg cgccggcgaa ggcgacacgg cgcgcccgct aacgcgccag
     3061 cgcgatcgtg tagcagcgct cggcgcgacc gcgctggtcg ttgccctcgt cgacgagctg
     3121 cgccgacggg aaccagcggc gaacctcgtc gccgtcgacg gagaacggtg ggccttcggc
     3181 aaagcccggc gggtagtcga tcgagacgag aagctcgcgc ttcgccatcg gcgtgatcgc
     3241 gcgcaggtgg gcgatgtagc ggctccggat gtcgggtggt agcgcgacga tcgcagcgcg
     3301 gtcgtagatg ccgtcgatcg tgccgacgag ctcgcgtgtc gtcgcgaaga aatcgcccgc
     3361 gatgatcgtg atgacgcccg cgcgatagat ctcgagtccg tcgcgctcgc tgatctccgg
     3421 cgttgctgcg tgctccgcaa agaactgccg caccgcatct tcgacgagct cgacgccgat
     3481 cacctcgtga ccgcggctcg cgagatacgc gaggtcttcc gtcttgccgc acagcggaac
     3541 cagaatgcgg tggcattgcg cgagccactc gccatgccga gccaggaacg cgttcgcctt
     3601 gccttcatgg aatgcggtgc ggccctcgtt ccatctcgca cgccagaact cgtgatccat
     3661 gcgcgcactg tacccgatgg gcgaacgcac ccgctttccg tcggatcacg tacgcgatgc
     3721 acccgcgcat agctcgtgca tcgtgtttgt cacggagaac accgatgagc attacctaca
     3781 gcaagtcgat catcgccgcg atcgtcctcg cagtggctgc taccggcaac gcgcaaccga
     3841 caaaggatga gacgcaggcg cgatcgcagt gctgcgtcca aaagacgtcg tcggtacagc
     3901 agagccaggt ggaagcgagg ccaacgccgc cccgtgccgc gaacccgcca aagatcgatc
     3961 cacaagcgat cgccgcgctc gagaagatgg gctgctttct gcgaaagcaa cagaacttca
     4021 cggtcagcgc cgacgcgcag accgactatg tgatccagaa cgatctattg gtgacactgc
     4081 cgaagcacgc cgagttgcgt gtgaagcgtc ccaacggctt gcgcatcgac gtgacgtccg
     4141 atcgcaagca gcggcagttc ttctacgacg gcaagacgtt cacgatgtac ggcgagaagg
     4201 ttggctacta cacgagcgtt ccggcgccgc cgacgatcca cgagctcgcg aacatgctcg
     4261 aggatcgtta tggcctgcag ctgccgctgg tggatctgtt ccgctggggc actgcccagt
     4321 cgccagtgaa ggagatcacc gcggcggtgc gtgtcggcac cgagcggatc gacggtgtca
     4381 tgaccgatca ctacgcgttc cgtcaaaagg gcgtcgactg gcagatctgg atcgagcagg
     4441 gcaatcggcc ggtgccgcac aggctcatcc tgacgaccac cgacgaccct gcgcgccccg
     4501 cgtacgcgct cgacatgcag tggacgctga acacgttgaa tgactggccg gtgttcacat
     4561 tcgtcgcgcc gcccgatgcg gttcggattc cgctgagcga acgcacgatt caaaccgttg
     4621 cacgcgccga gcaagccaag aagcgctcga gcgaacctac caagcaataa ccaacgagga
     4681 ccgacaatgt ttggcacacg ttttcaacga tcaagtttgc tggcgctcgg tctcgtcgtg
     4741 atcggcgccg gcctacgtat cgacgacgcc gacgcacgcg gtggacgcgg cggtggtgga
     4801 cgcgccggcg gcatgagtcg cggcggcggc ggtggcttca gccgcggtgg cggcggcatg
     4861 agtcgcggcg gcggcgctcg cacgagtctg agtagcagcc ctcgcggcgg cgcgagtcgc
     4921 ggcagctacg gcggaggtgc gagtcgcggc agctacggcg gcggtgcgag tcgcggcagc
     4981 tacggcggcg caagtcgcgg tagttacgca ggcggcggaa ccgcgagccg cggcggtgct
     5041 ggtgcctatg gcagccctgg gtatcaacgc ggtggtggcg ctgcggccca gcgcggtgct
     5101 gccggtgctg gcgcaactcg tccatcaggc ggcatggcgc agggcggtac cgcacgcccg
     5161 caagca
//
LOCUS       DASUFX010000659         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1077346, whole genome shotgun sequence.
ACCESSION   DASUFX010000659 DASUFX010000000
VERSION     DASUFX010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077346"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1479)
                     /locus_tag="VFV99_26725"
     CDS             complement(178..1479)
                     /locus_tag="VFV99_26725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063009.1"
                     /translation="MLAFECGRPRAGSVRYRLSDLTMVNLGRGSERRAEKAGTELTIK
                     VPDKWMSSKHARIEPSFGRWVLVDTESKNGSIVDGHTTKRAVLTDGSLIELGHTLFFF
                     FERMPIDADAVAMLEMTPTEAEPGLVTLVPGWQAELNRIRQIAGSEIPMLIEGESGVG
                     KEVIARAIHALSGRKGAFVPVNCGALPENLVESELFGYKKGAFSGAQQDHPGLVRAAD
                     GGTLFLDEIGDLPASSQAALLRVLQEKEVMPVGGTRPVAIDLRVVAATHRDLDEMVAE
                     QTFRHDLFARLAGFRILVPPLNERRGDLGLLIGALHGRLFPAEHPGFEIDAARLLLRY
                     PWPLNVRELEQALAAAQVLAGTEVVRAEHLPDTVRSGRPPGAPRPVVLSETDQKVRDQ
                     VVAALREHQGNVSAVARALDKDRKQIQRWIKRFGLDPSSYR"
     gene            1641..2132
                     /locus_tag="VFV99_26730"
     CDS             1641..2132
                     /locus_tag="VFV99_26730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063010.1"
                     /translation="MGDLVFLNLGPRRGMRKGTSALAWYEKGCALEASDPDGAVASYV
                     KALAGRPDFADAHNNLGRLLHDRGDLVAAEGHYRLALCTSRYMALYWFNLGVVLEDLG
                     RSAEAIDAYERALAIDTQLADAHFNIARQIERAARLANDEIMMYRAVRHLKQYRQLSR
                     ASG"
     gene            complement(2139..>2584)
                     /locus_tag="VFV99_26735"
     CDS             complement(2139..>2584)
                     /locus_tag="VFV99_26735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063011.1"
                     /translation="SITHVDQSQRALDFAATHHVVDRAKHHFVTADIFTWLPALAADE
                     QFDVVIVDPPAMTSRKTQVPSVLAAYRKLYRAAESHVRPGGMLVSACCTSRVERGVFR
                     KTVRDALPGRFSLERDLPPEPDHPVGFAQADYLKILLWRAAPSPI"
BASE COUNT          429 a          851 c          884 g          420 t
ORIGIN      
        1 agaatcacgc gcgcgcattc acgcgtgcgg cgtcgcgcga cagtcgcagc gacgccgcat
       61 gtcgcgggat cggcagcgtt gcgcgatccg atcgcgtgcg tcgcgcagga tggcaacgat
      121 ctgcctgttg gcaagtgcca ccgtctgcgc gagcgagatg gccgcgctcg cgacgcccta
      181 gcggtagctc gagggatcga gaccgaagcg tttgatccag cgctggatct gtttgcgatc
      241 tttgtcgagc gcgcgagcga cggcggacac gttgccctga tgctcgcgca gcgcggcgac
      301 gacttgatcg cgcaccttct gatcggtttc cgacagtacg accggacgcg gtgcacccgg
      361 tggtcgaccc gagcggacgg tgtcgggcag atgctcggcg cgcacgacct cggtaccggc
      421 gagtacctgc gcagcggcga gcgcttgctc gagctcgcgc acgttgagcg gccacggata
      481 gcggagcagc aggcgcgccg catcgatctc gaacccggga tgctcggcgg gaaacagtcg
      541 cccgtgcagt gcgccgatca agaggccgag gtcgccgcgg cgctcgttga gcggcggcac
      601 caggatccgg aacccggcga ggcgcgcgaa cagatcgtga cggaacgtct gctccgcgac
      661 catctcgtcg agatcgcgat gcgtcgccgc gacgacgcgc agatcgatcg cgaccggacg
      721 cgtgccgccg accggcatca cttctttttc ctgcagcacg cggagcagcg ctgcttgcga
      781 gctcgcgggc aaatcgccga tctcgtcgag gaagagcgtg ccgccatcgg ccgcgcggac
      841 gagaccggga tggtcctgct gtgcgccgga gaacgcgccc ttcttgtagc cgaacagctc
      901 gctctcgacg aggttctcgg gcagcgcgcc gcagttgacc ggcacgaacg cgcccttgcg
      961 tcccgatagc gcgtggatcg cgcgcgcgat cacttccttg ccaacgccgc tctcgccttc
     1021 gatcagcatc ggaatctcgc tgcctgcgat ctggcgaatg cgattgagct cggcttgcca
     1081 acctggaacg agggtgacga gaccgggctc ggcttcggtc ggcgtcatct cgagcatcgc
     1141 tactgcatct gcatcgatcg gcatgcgctc gaagaagaag aacagcgtgt ggccgagctc
     1201 gatgagcgag ccgtcggtga gcaccgcgcg cttcgtcgtg tggccgtcga cgatcgaacc
     1261 gttcttcgat tcggtgtcga ccagcaccca gcggccgaag ctcggctcga tgcgcgcgtg
     1321 cttggacgac atccacttgt cgggcacctt gatcgtcagc tcggtgccgg ctttttcggc
     1381 gcggcgctcg gagccacgcc cgaggttgac catggtcaga tcgctgagcc gatagcgcac
     1441 cgagcccgcg cggggccggc cgcattcgaa tgcgagcacg aggacaggtt cgggacgcgg
     1501 accaacccgg accccttttc catccgcatg gatactcagc gtcgtcccgt gcgtgtcacc
     1561 cactctgggg gccatcctaa cagaggtgtc gcacccctgg ggatcgggct ttgtcagacc
     1621 cggtcgatag ggtggcagcc gtgggcgatc tcgtcttcct gaacctcggc ccccgccgcg
     1681 ggatgcggaa gggcaccagt gcactcgcct ggtacgaaaa aggctgcgcg ctcgaagcca
     1741 gcgacccgga cggtgcggtg gcgtcatatg tcaaagcgct cgccggccgc cccgacttcg
     1801 cggacgcgca caacaacctc ggccgtctgc tccacgatcg cggcgacctc gtcgcggcag
     1861 agggacacta tcggctggcg ctgtgcacga gccggtacat ggcgctctac tggttcaacc
     1921 tcggggtcgt gctcgaagat ctcggccgca gcgccgaggc gatcgatgcg tacgagcgtg
     1981 cgctcgcgat cgatacccag ctcgcggacg cgcacttcaa tatcgcccgg cagatcgagc
     2041 gcgcggcgcg gctcgcgaac gacgagatca tgatgtaccg ggcggttcgc cacctgaagc
     2101 agtaccgcca gctgtcccgc gcctcgggct agatcgggct agatcgggct tggcgccgcg
     2161 cgccagagca agatcttcaa gtagtcggcc tgcgcgaagc cgaccggatg atcgggctcg
     2221 ggcggtagat cgcgttcgag cgagaaccga ccgggaagcg cgtcgcgcac ggtcttgcga
     2281 aacacgccgc gctcgacgcg actcgtgcag catgcgctga cgagcatgcc gcccgggcgc
     2341 acgtgcgact cggcggcgcg gtacagcttg cgatacgcgg caagtacgct cggtacctgg
     2401 gtcttgcggc tcgtcatcgc cggcggatcg acgatcacga catcgaactg ctcatccgcg
     2461 gcgagcgccg gcagccacgt gaagatgtcg gcggtgacga agtgatgctt cgcgcgatcg
     2521 acgacgtgat gcgtcgccgc gaaatcgagc gcgcgctgcg attgatcgac gtgcgtgatc
     2581 gatg
//
LOCUS       DASUFX010000660         4346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1077349, whole genome shotgun sequence.
ACCESSION   DASUFX010000660 DASUFX010000000
VERSION     DASUFX010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077349"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..236
                     /locus_tag="VFV99_26740"
     CDS             39..236
                     /locus_tag="VFV99_26740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063012.1"
                     /translation="MRRPLYLELCKSELWEGVRCTLHAGHQGSHQRPATDTSCAISWR
                     SSTFPPPLPVKRASKFRMITH"
     gene            complement(233..598)
                     /locus_tag="VFV99_26745"
     CDS             complement(233..598)
                     /locus_tag="VFV99_26745"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5063013.1"
                     /translation="MPRYEGSCHCGALRFAVELADDERDVLDCNCSICTKKGFLHLIV
                     DESRFTLLAGTPAVYTFGTHTAKHMFCATCGMQPFYRPRSHPNAWDVNVRCLDIPLTH
                     WRIRPFDGANWEDNVDSIR"
     gene            640..1713
                     /locus_tag="VFV99_26750"
     CDS             640..1713
                     /locus_tag="VFV99_26750"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEX5063014.1"
                     /translation="MSDSAELSAALEAALVRELRRLYEQLNERWFNERLNRPVLALAD
                     TTRRLGQWTRATRMLELSRTLVFARPWREVIGVLEHEMAHQYVDEVLGVLDETPHGPT
                     FQRVCAERGIDARAAGMPMESQTDVDSPGDRALDKIRKLLALAGSDNQNEAEIAMRRA
                     HELMLRHNIEQAATERHYEVRHIGTPSLRANAVEADIVGLLTEFFFVEVIRVGTYLPH
                     EGRQAHIYEVMGTHANVEMAVHVFQFLLATADRLWRENRGDARVRNGRDRLSYQSGVI
                     RGFREKLLAERKTLKGTGLVWVGDGALHDFYRARYPRIRTVKRSVRLSRAHDAGREAG
                     RNVILHKPVSDTSTRAKQRLLRG"
     gene            1828..2343
                     /locus_tag="VFV99_26755"
     CDS             1828..2343
                     /locus_tag="VFV99_26755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063015.1"
                     /translation="MKLANLLPLLLLAACTDNATKQESVQIFAGATTAMTSAQSRAVD
                     QARGNHLNAPADLTLDYSGPCTLGGTVALSGDYTGDSAVDDRAAFDLKATFNGCHELT
                     GTLDGDLQWTSVATGDSFTATMTGGLNWKGNDGEASCDFDLSLSVGTTGVSYDGHLCG
                     YDVKTEIVLGH"
     gene            complement(2344..2868)
                     /locus_tag="VFV99_26760"
     CDS             complement(2344..2868)
                     /locus_tag="VFV99_26760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063016.1"
                     /translation="MRPVWVLMALAACGSSMPAGGDAGGDGNDANLDDANDICSGQCK
                     NTSVALMFQTNRTLDSAYYGTTFDATTLRIEAYRKANPGCPTTGMANPDYKLILGEVN
                     PATPDSNMSTANIVDLKGDLLGGPLGLAATGKQITLVAAVDGSFIAVDVMLTFSSGTA
                     MGHIYATHCATLDM"
     gene            2892..3785
                     /locus_tag="VFV99_26765"
     CDS             2892..3785
                     /locus_tag="VFV99_26765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063017.1"
                     /translation="MKALILVVVALAAVACGSKKQDKPAEKPVETKTCPAGQVDKDGT
                     CTVIVTQQQVAAVAQQQSRIDELAKLLDQVDTLAAPVDVLNGLRDIDAWKALVASNDK
                     AKLADDLVAQLQNGVKTLRTFKASLGEVQTRVGNLKGELDKLMTQTGAAQKFADVQAQ
                     VSAQIKATVEPFAAQVTDTIQNAIVPLSAKLDEVSAVVDIACGTVKLKGGDKAKALCK
                     DAKDAFAKATTFITDFKSKPAALYAELSTTLQSQLEQLVDKETKLALDTAQAKVNDAL
                     KLPAAGSAAPAGVAAGSGSAQ"
     gene            complement(3786..>4346)
                     /locus_tag="VFV99_26770"
     CDS             complement(3786..>4346)
                     /locus_tag="VFV99_26770"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5063018.1"
                     /translation="KKRCVWATREEFWDYHDHEWGVPVHDDQKHYEFLILEGAQAGLS
                     WSTILNKRDGYRRVFAGFDPAKVARFTPARIEKALLDPGIVRNRQKVTSAVNNARHFL
                     DLVEREGSFDKFIWSFVGGKQIVNRWAHTKQIPATSKESDALAKELKKRGFSFVGSTV
                     MYAHMQATGLINDHLVDCFRYKAVTR"
BASE COUNT          808 a         1346 c         1447 g          745 t
ORIGIN      
        1 ggcgcggtcc ccgaattacg gagtgaccgc caatgccgat gcgtagacct ctctacttgg
       61 agctgtgcaa gagcgagctc tgggaaggcg tgcgttgcac gttgcacgcg ggccatcagg
      121 gcagccatca gcgaccagca accgacacga gctgcgcgat cagctggcgt tcgtcgacgt
      181 ttccgccgcc gctgccggtc aagcgcgcgt cgaagttccg gatgatcacc cactagcgga
      241 tcgaatcgac gttgtcttcc cagttggcgc cgtcgaatgg acgaatgcgc cagtgagtga
      301 gcggaatgtc gaggcagcgc acattgacgt cccacgcatt gggatgcgaa cgcgggcgat
      361 aaaacggctg catgccgcac gtcgcgcaga acatgtgttt cgcggtgtgc gtgccgaacg
      421 tgtagacggc gggcgtgccg gcgagaagcg tgaagcgaga ctcgtcgacg atcaggtgca
      481 agaagccctt cttcgtgcag atcgagcaat tgcaatcgag cacgtcgcgt tcgtcgtcgg
      541 cgagctcgac ggcaaaccgg agcgcgccgc agtggcagct accttcatag cgaggcacga
      601 ctggagaatg tcagggcgtt cgggcatcgt catcctccga tgtctgattc ggccgagctg
      661 tcagcggccc tcgaggccgc gctggtccgc gagctgcgcc gactttacga gcagctcaac
      721 gagcgctggt tcaacgagcg gctgaaccgt ccggtgctcg cgctcgccga cacgacgcgg
      781 cgactcggcc agtggacgcg tgcaacgcgc atgctcgagc tgtcgcgtac gctcgtgttc
      841 gcgcggccgt ggcgcgaggt gatcggtgtg ctcgagcacg agatggcgca ccagtatgtc
      901 gacgaggtgc tcggcgtcct ggacgagacg ccgcacgggc cgacgtttca gcgcgtgtgc
      961 gccgagcgcg gcatcgatgc gcgagccgct ggcatgccga tggagtcgca gaccgatgtc
     1021 gactcgccag gggatcgtgc gctcgacaag atccgcaagc tgctcgcgct tgccggtagc
     1081 gacaaccaga acgaggccga gatcgcgatg cgccgcgcgc acgagctgat gctgcgccac
     1141 aatatcgagc aggcggccac cgagcggcac tacgaggtcc gccacatcgg cacgccgagc
     1201 ctacgtgcga acgccgtcga ggctgacatc gtcggcctgc tcaccgagtt ctttttcgtc
     1261 gaagtgattc gcgtcggcac gtatctgccg catgaaggcc ggcaggcgca catctacgaa
     1321 gtgatgggga cgcacgcgaa cgtcgagatg gcggtgcacg tgtttcaatt cttgctcgcg
     1381 accgccgacc ggttgtggcg cgagaaccgt ggcgacgcgc gcgtgcgcaa tggccgcgat
     1441 cggctctcgt atcaatcagg cgtgatccgc ggctttcgcg agaagctgct cgccgagcgc
     1501 aagacgctca aaggcaccgg gctcgtgtgg gtcggcgacg gcgcgttgca cgacttctat
     1561 cgcgcgcggt atccgcgcat ccggaccgtg aagcgcagcg tgaggctcag tcgcgcgcac
     1621 gatgcgggcc gcgaggcggg tcgcaatgtg atcttgcaca agccggtgag tgacacgagc
     1681 acgcgcgcta agcagcgatt gctgcgcggt tagtgtgcat ctgaatcacg gtcggcagcg
     1741 tgatgccagc ctttgctagc ccgcgtcggg accggtgtcg gcgaggctag ggcaggattt
     1801 ccgtcgctta gatctaagga gataacaatg aagctagcca acctacttcc gctgctcttg
     1861 ctcgccgcct gcaccgacaa cgcgacgaag caggagtccg tgcagatctt cgctggtgcg
     1921 accaccgcga tgacctcggc gcagtccagg gcggtcgatc aggcccgcgg caatcacctc
     1981 aacgcgcccg ccgatctgac gctcgattac tcgggcccgt gcacgctcgg cggcacggtc
     2041 gcgctcagcg gcgactatac gggtgacagc gcggtcgacg atcgcgcggc attcgatctg
     2101 aaggcgacgt tcaacggttg ccacgagctg accggcacgc tcgacggcga tctgcagtgg
     2161 acctcggtcg cgaccggcga cagcttcacc gcgacgatga ccggtggctt gaactggaag
     2221 ggcaacgacg gcgaagcgtc gtgcgacttc gatctgtcgc tgtccgtcgg cacgaccggc
     2281 gtgagctacg acggccacct ctgtggctac gacgtgaaga ccgagatcgt tctcggccac
     2341 taactacatg tcgagcgtcg cgcagtgggt cgcatagatg tgacccattg cggttccgct
     2401 cgagaacgtc agcatcacgt cgaccgcgat gaagctgccg tcgacggcgg caacgagcgt
     2461 gatctgcttg ccggtcgcgg caagcccgag tggaccgccg agcaggtcgc ccttgaggtc
     2521 gacgatgttc gcggtcgaca tgttcgagtc cggcgtcgcc ggattgacct cgccgaggat
     2581 gagcttgtag tcgggattcg ccatcccggt tgtcgggcaa ccggggttcg ccttgcggta
     2641 ggcctcgatc cgcagcgtcg tcgcatcgaa cgtcgtgccg tagtaggcgc tatcgagcgt
     2701 ccggttcgtc tggaacatca gcgcgacgct ggtgttcttg cattggccgc tgcagatgtc
     2761 gttcgcgtcg tcgaggttcg cgtcatttcc atcaccgccg gcatcaccgc ccgcgggcat
     2821 ggatgatccg catgccgcga gcgccatcaa gacccatacc ggacgcaccc gaacatgata
     2881 accctgcccg tatgaaagca ctcattcttg tcgtggtggc ccttgctgcc gttgcgtgtg
     2941 gcagcaagaa gcaggacaag cctgcagaaa aaccggtcga aacgaagacg tgtcccgccg
     3001 gccaggtcga caaggatggc acctgcacgg tgatcgtcac acagcagcag gtggccgcgg
     3061 tcgcacagca acagtcgagg atcgacgagc tcgcgaagct gctcgatcaa gtcgacacgc
     3121 tcgccgcacc ggtcgacgtg ctgaacggcc tgcgcgacat cgatgcatgg aaggcgctcg
     3181 tcgcgtcgaa cgacaaggca aagctcgccg acgatctcgt cgcgcagcta cagaacgggg
     3241 tgaagacact gcgcacgttc aaggcgagcc tcggcgaggt gcagacgcgc gtcggcaatc
     3301 tcaagggtga gctcgacaag ctgatgacgc agaccggcgc ggcgcagaag tttgcggacg
     3361 tgcaggcgca ggtctcggcg cagatcaaag cgaccgtcga gccgtttgcc gcgcaggtca
     3421 ccgatacgat ccagaacgcg atcgtaccgc tcagcgcgaa gctcgacgag gtatcggcgg
     3481 ttgtcgacat cgcgtgcggc acggtgaagc tcaagggcgg cgacaaggcg aaggcgctgt
     3541 gcaaggacgc gaaggatgcg ttcgccaagg cgacgacgtt catcaccgac ttcaagagca
     3601 agcccgctgc gctctacgcc gagctctcga cgacgctgca gagccagctc gagcagctcg
     3661 tcgacaagga gacgaagctc gcgctcgaca ccgcgcaggc gaaggtcaac gacgcattga
     3721 agctgccggc agcgggctcg gcggcgcctg cgggcgtcgc cgcgggctcc ggttccgcgc
     3781 agtagctagc gggtgacggc cttgtagcga aagcagtcga cgaggtgatc gttgatgagg
     3841 ccggtcgcct gcatgtgcgc gtacatcacc gtcgagccaa cgaacgagaa gccacgcttc
     3901 ttgagctcct tcgcgagcgc gtcggactct ttcgacgttg caggaatctg cttggtgtgg
     3961 gcccagcgat tcacgatctg cttaccgccg acgaagctcc agatgaactt gtcgaagctg
     4021 ccttcgcgct cgacgaggtc gaggaagtga cgcgcgttgt tcaccgctga cgtgaccttc
     4081 tggcggtttc gcacgatgcc gggatcgagc agagccttct cgatgcgcgc cggcgtgaag
     4141 cgcgcgacct tcgccggatc gaatcccgcg aacacgcggc gatagccgtc acgcttgttg
     4201 aggatcgtcg accacgacag gccggcttgt gcgccttcga ggatcagaaa ctcgtagtgc
     4261 ttctgatcgt cgtgcaccgg tacgccccat tcgtggtcgt ggtagtccca gaattcttcg
     4321 cgagtcgccc agacacaacg tttctt
//
LOCUS       DASUFX010000661        10110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_103949,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000661 DASUFX010000000
VERSION     DASUFX010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103949"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFV99_26775"
     CDS             complement(<1..736)
                     /locus_tag="VFV99_26775"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063019.1"
                     /translation="MRAIWLVILTACSLNVDYTGTNYQCNPDGSCPPDFECQDMVCVP
                     TDPVPPACSQDVSSGAAHACAVRNDGTVWCWGANSSGQLGDGTVTDSDVPVQVASVMK
                     AVKVGAGDQSSCALTEAGEVFCWGSNGSGQLGDGTNTDNRTPVQVRDVSGAKQLTVGA
                     AHACVLKSDNTIACWGSNQDGQIGDGSTTARNSPTAAMIGAARSVTAGSDITCAVLMD
                     GTPQCWGENNDGELGVGDANPHPMPTT"
     gene            complement(736..1779)
                     /locus_tag="VFV99_26780"
     CDS             complement(736..1779)
                     /locus_tag="VFV99_26780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063020.1"
                     /translation="MRSLWGQVAIAAALLTTLARDARADALADARKAVEGSDYMAAQP
                     ALETALKAGTAGPAELAEIYKLTGIVEAALGNAGPSEKAFAKWLALDPKGALPEGTSP
                     KITRPFDAAHAQANKSGPLEAKAETQDTPPAVTLKVANDPQKMIVAAKVYFIVDGKSE
                     QTLSADGRNEITIELGLGKRIDLRIHGLDRYGNRVVELGSKDVPIVITSSGKETPVGS
                     DRVGHAKPKTVEPEKPRPWYLQWWLWGGATVAVTGVGGYFAYRTYDNMQTIEHLHATS
                     LDHPWSDEQSVERSARRNLLVTNIAAGTAGAFAIVTTILFLTRPNPESESAMRATLTP
                     THGGAAVVLGGHF"
     gene            complement(1790..3847)
                     /locus_tag="VFV99_26785"
     CDS             complement(1790..3847)
                     /locus_tag="VFV99_26785"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063021.1"
                     /translation="MEAASTASEARRFGKYTLVAKLATGGMAEIFLARLHGDGGFEKL
                     VCIKRILPHLAKDPQFVAMFLDEARVAARISHPNVCQVFELGECEGQYYIAMEYLEGV
                     PLSLFRRRDFYPGLPDPRLVAGFGVQACEGLHHAHQLKRTDGELLGVVHRDISPQNLF
                     ATTDGIVKVLDFGIAKVQDASVRTSTGAVKGTYAYMAPEQLRNEKLDRRVDVWALGVV
                     LWETLAQQYLFRRDTEFLTFEAITKLPIPDLHEVRPDVPPLLADTIARALSRNPDDRF
                     ASARALGEALGQAVGPLGGIAQSATISDEVSRSFEATLAEQRMLLRIAREGGQFDLER
                     SSSLVAHGTSMSATPVSNIHRRVEEAKAEVAQFVAASQAIDSLYEDEGMKSRPHRFGT
                     DGAFTPAPSRRSGGQDAVPDESGIIVKHASEPMRAQSVSGPYAVAPAEWRYDETAPPV
                     AVPPRMSGAVAMQAPRGSRLPWMIATVAIASIAAAAAIFYMEVREQKSATPAQAQPEH
                     TEAVVMNATAPTPPPAAPVVAEVSQDAAAALPSADENKVTPPSDVEAKKADTKRDKSN
                     KTARVEKKVDKVGEVKKADKKPDKIVEKKQDKVEKTVEKTPEPVKAVAQVGPPGTITI
                     DSAPVYAVIYIDGKKYGETPVVNIKLAAGKHSVRAVSPSGSTQNLTINIESGKSSVRR
                     IGW"
     gene            complement(3864..6470)
                     /locus_tag="VFV99_26790"
     CDS             complement(3864..6470)
                     /locus_tag="VFV99_26790"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX5063022.1"
                     /translation="MRRLGLFLVLTSAAAYADTLPAEVRAHATRRTGSISINGKLDEA
                     AWASAPRQGGFTQRFPKDGAKASHDTQFAVLYDDEAIYVGVWASDPEPGKIRRTLTRR
                     DVDALADAIAIGFDSYHDKRTAYVFQINAAGVQRDMMLFDDMAQDDTWDAVWTGDVAI
                     TPEGWTAEVRIPLSQLRFAGGETHEWGLQVVRMIARNQEQSAWSPWPRTGNQIVSRFG
                     IVEGIEVNKSARRLELLPYATGGVETQPIDAGDPLNPETSALGNAGVDVKFGLGPAFT
                     LSATINPDFGQVEADPSQVNLSANELFFAEKRPFFLEGVDLFKLPIGSGDNLIEGAFY
                     SRRIGAAPAEPDMDYAFIDAPTSTTIYSAAKVTGKTNSGWSVGVLDAVTGQENATIVD
                     DAGTKMNPVVAPLTNYAVGRVKRDLHGGRTTIGASATAVNRSLDGTPLVDINHDQAYT
                     AGAQFTHRWGDNAWELDVHGVGSYVHGTEAAIANTQQLNRHLFQRPDASNVMLDPTRT
                     SLSGLGMSWFVGRSGETKHVRWAQGGDLRTPGLELNDAGFQMYSDRVDPWLWGEYRED
                     DPGDTVLNWRAQSILFSSHDMEPRLLTYGVDFNASAQLANYWFVRNGWHVEAGGWDTT
                     ALRGGDALRTDTRLASTAGISTDSRKDVRGEFWVNGNRNFAADAMQVNTGGAVYVQAR
                     SNIDIWTALDWTQRTDPMQYIDQIEDDAGRTHYVFGTIAQTTVSVTTRVNWTFSPKLS
                     LQVYAQPFVAAGNYSELKDVDNPHAKRFSDRFHMLSGNDYTEMDGTVTVSHNGNYSFD
                     RPDFNIGELRSTVVLRWEYRPGSAVFAIWSHGRSNSVDDGAFDLAKDVTDLGRAPGEH
                     IVMVKANYWIGL"
     gene            complement(6585..7673)
                     /locus_tag="VFV99_26795"
     CDS             complement(6585..7673)
                     /locus_tag="VFV99_26795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5063023.1"
                     /translation="MPKHRGRDLRLVSRCRVEFDRPSGPVEAETEDLSARGMFIRTDA
                     LLPVGEETEIRLTLPDSTFLAMRARVAHMLTPVAARALGRHPGMGFELIGVDTPSRIK
                     LRAHVDNVRVETTNPGLTTTTSAIIVEPTGPLRERMVRCLEGAGFKIIAVASMPEALD
                     ACTQWRPDVIISAAVLDHGMSGVDLAYAMSEHTVLADVPLVLTGDEGDLARLEAFRAG
                     IRDYIPRPFLDEELVIRVHRVVAPVAPIANPGLRGNLVDIGLGTLLSLFEFERKSGIL
                     LLLRDGDIARLFVSEGKVLKVEASTGNGAPKQRLMALLDWHAGQFEFSPCSIGGRDEL
                     NTSITQLLLEHARVSDEQMPERTSKVRR"
     gene            7825..8886
                     /locus_tag="VFV99_26800"
     CDS             7825..8886
                     /locus_tag="VFV99_26800"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5063024.1"
                     /translation="MTVAYESVTSVGAAVLDRLANISGARGAIDLRQRLAALGRWVRA
                     DLADFEAELATIPRGARVVQQAAHHLLDLRGKHLRPLCVALASRFGEGFTPRARGLAV
                     AVELVHTATLLHDDVVDVAERRRGQPAAAIVYGNAASIFAGDWLLVAALRRISATGVD
                     GVLEKMLEVIDEMIMAESLQLERRGRVVADRDEYFRIVEGKTAALFRWAMLAGARCAG
                     MAAEHEAVLEKYGVHLGVAFQAVDDELDFAGLEATGKDPLADLREGKMTYPLIVALER
                     ESSLRARLEAVLGEDVPSPLELTAIAAAVRATGALQATRELAEQHVQLALETLAALPA
                     GTARESLETVALASLERTS"
     gene            8883..9686
                     /gene="ubiE"
                     /locus_tag="VFV99_26805"
     CDS             8883..9686
                     /gene="ubiE"
                     /locus_tag="VFV99_26805"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX5063025.1"
                     /translation="MSQLAHQPESLPTLAPQQLPTLGSGEMFDRIAARYDFVNRVLSL
                     GLDQRWRRRVVRSLGLETRKRFGRKDVRGQWFGERPRVLDVATGTGDLAIEIARACPG
                     ASVIGLDPSGGMLGVAKQKLAKRGLSDRVELVIGDAQHLPQKNCEIDAATIAFGIRNV
                     PDRGQALRELARVVRPGGRIAVLELGEPRRGLLAAAARLHCHHIVPKLGGWLSGSREY
                     EYLQRSVAAFPPAEEFADLMRASGLDVIEVVPLTFGVCTLYLATPAEER"
     gene            9688..>10110
                     /locus_tag="VFV99_26810"
     CDS             9688..>10110
                     /locus_tag="VFV99_26810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063026.1"
                     /translation="MKAVPDMFMERIGDVAHAFALRDADDDLDAGLAIARRQAGWTFI
                     ALPAPVVAAPGLVAAWRSTPVVAWSSSALTIVGVGTAHELRGRGGARWREVISGAARV
                     EHTTLVDRSGTGSLARPRYLGGLAFAPGAADGAPWQGFG"
BASE COUNT         1599 a         3373 c         3308 g         1830 t
ORIGIN      
        1 ccgtcgtcgg catcggatgc ggattcgcat cgccgactcc gagctcgccg tcgttgtttt
       61 cgccccagca ctgcggtgtg ccgtccatca acaccgcgca cgtgatgtcg gagcccgcgg
      121 tgaccgagcg tgccgcgccg atcatcgctg cggtcggcga gttgcgcgca gtcgtgctgc
      181 catcgccgat ctggccgtcc tggttgctgc cccagcacgc aatggtgttg tcgctcttga
      241 gcacgcacgc atgcgcggcg ccgaccgtga gctgcttcgc gccgctcacg tcgcgcacct
      301 gcaccggcgt gcggttgtcg gtgttcgtgc catcgccgag ctgccccgaa ccgttgctgc
      361 cccagcagaa cacttcgccc gcttcggtga gcgcgcagct cgactggtcg ccggcgccga
      421 ccttgaccgc cttcatcacg ctcgcgacct gcaccggcac gtcgctatcg gtcacggtgc
      481 cgtcgccgag ttgaccggag ctattcgcgc cccagcacca gaccgtgccg tcgttgcgca
      541 ctgcgcacgc atgagcggca ccggacgaga cgtcctgcga gcacgccggc gggacaggat
      601 cggtcggcac gcacaccatg tcctggcact cgaagtcggg cggacacgag ccatcgggat
      661 tgcattgata gttcgtgccc gtgtagtcga cgttcaggga gcacgcagtg agaatgacca
      721 gccagatcgc tcgcatcaga agtgccctcc tagaacaacg gcagcaccac catgtgtcgg
      781 cgtgagcgtc gcgcgcattg cggattcaga ttccgggttc ggtcgcgtca ggaacaagat
      841 cgtcgtgacg atcgcgaatg cacctgcggt tccggcggca atgttcgtaa cgagtaggtt
      901 acggcgcgcc gagcgctcga cgctctgctc gtcgctccac ggatgatcga ggctcgtcgc
      961 gtggaggtgc tcgatcgtct gcatgttgtc gtacgtgcgg tacgcgaagt aaccgccgac
     1021 gccagtgacc gcaacggtcg cgccgcccca cagccaccac tgcaggtacc acgggcgtgg
     1081 tttctctggc tcgacggtct tgggtttcgc gtgtccgacg cgatcggatc cgaccggcgt
     1141 ttccttgccc gagctcgtga tcacgatcgg cacgtccttc gagccgagct cgacgacgcg
     1201 attgccgtag cggtcgaggc catgaatgcg gagatcgatt cgcttgccga ggccgagctc
     1261 gatcgtgatc tcgttcctgc cgtcggcgga tagtgtttgc tccgacttcc cgtcgacgat
     1321 gaagtagacc ttcgccgcca cgatcatttt ctgcggatcg tttgcgacct tcagcgtcac
     1381 ggccggcggc gtgtcttgcg tctcggcctt cgcctcgagc ggtccgctct tgttggcttg
     1441 cgcgtgcgct gcatcgaacg gtcgcgtgat cttcggcgac gtgccctcgg gcagcgcgcc
     1501 tttcggatcg agcgcgagcc acttcgcgaa cgccttttcc gacggtcccg cgttaccgag
     1561 cgcagcttcg acgatgcccg ttagcttgta gatttcggcg agctcggcag ggcccgcggt
     1621 gcccgctttg agcgcggtct cgagcgccgg ctgcgcggcc atgtagtccg agccttcgac
     1681 ggccttgcgt gcgtcggcga gtgcgtccgc acgcgcgtct cgcgcgagtg tggtcagcag
     1741 cgcggccgcg atcgcgacct gcccccacag ggacctcatt cgcgagagct taccacccga
     1801 tgcgtcgaac cgatgatttt cccgactcga tattgatcgt gaggttctgc gtcgatccag
     1861 acggcgaaac tgcgcgaacg ctgtgtttac ctgccgcgag cttgatgttc acgacgggcg
     1921 tttcgccgta cttctttccg tcgatgtaaa tcaccgcgta caccggtgcc gaatcaatcg
     1981 tgatcgtgcc tggtgggcca acttgcgcca ccgccttcac cggttctggc gttttctcga
     2041 ctgtcttctc gaccttgtcc tgcttcttct cgacgatctt gtcgggcttc ttgtcggcct
     2101 ttttgacctc gccgaccttg tcgaccttct tctcgacgcg cgccgtcttg ttggacttgt
     2161 cacgtttcgt gtcggccttc ttcgcttcga cgtcggacgg tggtgtgact ttgttctcgt
     2221 cggcgctcgg tagggcggca gctgcatcct gcgaaacctc ggccaccacg ggggccgcag
     2281 gcggcggtgt gggggccgtc gcgttcatca cgaccgcttc ggtgtgctcg ggctgtgcct
     2341 gcgcgggcgt cgcggatttc tgctcgcgca cctccatgta aaagatcgcc gcggctgctg
     2401 cgatggatgc gatcgcaacc gtcgcgatca tccacggcag gcgcgagcca cgcggcgcct
     2461 gcatcgccac ggcgcccgac atacgtggcg gcaccgcgac gggtggcgcg gtctcgtcat
     2521 agcgccactc ggccggcgca accgcgtatg gacccgacac gctctgcgct cgcatcggct
     2581 ccgatgcgtg cttcacgatg atgccggact cgtcgggaac ggcgtcctgt ccgccggagc
     2641 gccggctcgg tgcgggcgta aacgctccgt cggttccaaa ccgatgcggg cgcgatttca
     2701 tgccctcgtc ttcgtacagc gagtcgatcg cttgcgatgc cgcgacgaac tgcgcgactt
     2761 ccgcttttgc ctcctcgacc cggcgatgga tgttcgacac cggcgtcgcc gacatcgacg
     2821 tgccgtgcgc gacgagcgag ctcgatcgtt cgagatcgaa ctggccaccc tcgcgcgcga
     2881 tgcggagcaa catacgttgc tcggcaagcg tggcctcgaa cgagcgcgag acctcgtcgc
     2941 tgatcgttgc cgactgcgcg ataccgccga gcgggccgac ggcctgaccg agcgcttcac
     3001 cgagcgcgcg cgcactggcg aaccgatcgt ccgggttgcg cgacagcgct cgcgcgatcg
     3061 tgtctgccaa cagcggcggc acgtcggggc gtacctcgtg cagatccgga atcggcagct
     3121 tcgtgatcgc ttcgaacgtc aagaactcgg tgtcgcgcct gaacagatac tgctgcgcga
     3181 gcgtctccca caacacgacg ccgagcgccc agacgtcgac gcggcgatcg agcttctcgt
     3241 tgcgcagctg ctcgggcgcc atgtacgcgt acgtgccttt gaccgcgccc gtgctcgtgc
     3301 gcaccgatgc gtcctgcacc ttcgcgatgc cgaaatcgag caccttcacg atgccgtcgg
     3361 tcgtcgcgaa cagattctgc ggcgagatat cgcgatggac gacgccgagc agctcgccat
     3421 cggtgcgctt cagctgatgc gcgtgatgaa ggccttcgca cgcttgcacg ccgaagccag
     3481 cgacgaggcg cggatccgga aggcccgggt agaagtctct gcgacggaac agcgagagcg
     3541 ggacgccctc gaggtactcc atcgcgatgt agtactggcc ctcgcattcg ccgagctcga
     3601 acacctggca cacgttcggg tgcgaaatgc gcgcggcgac gcgtgcctcg tcgaggaaca
     3661 tcgcgacgaa ctgcggatcc tttgcaaggt gcgggaggat ccgcttgatg caaaccagct
     3721 tctcgaaccc gccgtcacca tggaggcgag cgagaaagat ctcggccatg ccgccggtcg
     3781 ccaacttcgc gacaagcgtg tacttcccga agcgtcgcgc ttcggaggcc gtgctggccg
     3841 cctccatccc ggcgagtata gcgctacagg ccgatccagt agttggcctt gaccatcacg
     3901 atgtgctcgc cgggcgcacg tcctagatct gtaacgtcct tcgcgagatc gaacgcgccg
     3961 tcgtcgacgg aattgctccg gccatggctc caaattgcga agacagctga ccctgggcgg
     4021 tactcccacc tcagcaccac cgtcgaccgg agctcaccga tgttgaaatc aggacgatcg
     4081 aacgagtagt taccgttgtg cgacacggtc acggtgccgt ccatctcggt gtagtcgttg
     4141 ccggagagca tgtggaagcg atcggagaac cgcttcgcgt gtggattgtc gacgtccttg
     4201 agctcgctgt aattacctgc cgcaacaaac ggttgcgcat acacctggag cgagagcttc
     4261 ggtgagaacg tccagttcac acgggtcgtc accgaaaccg tggtctgcgc gatggtgccg
     4321 aacacgtaat gcgtgcggcc cgcgtcgtct tcgatctggt cgatgtactg catcggatcg
     4381 gtgcgctggg tccaatcgag agcggtccag atatcgatgt tcgaccgcgc ctgcacgtag
     4441 acggcaccgc cggtgttcac ctgcatcgcg tcggccgcga agttccggtt accgttgacc
     4501 cagaactcgc cacgcacgtc cttgcgcgag tccgtggaga tgccagcggt cgatgctagg
     4561 cgcgtgtctg tgcgcagcgc gtcgccgcca cgcaacgcgg tcgtgtccca gccgccagcc
     4621 tcgacgtgcc agccgttgcg aacgaaccaa tagttcgcga gctgcgcgga ggcgttgaag
     4681 tcgacgccat acgtgagcag gcgtggctcc atgtcgtgcg acgagaacaa gatgctctgc
     4741 gcacgccagt tgagcacggt gtcgcccgga tcatcttcgc ggtactcgcc ccacagccat
     4801 ggatcgacgc gatccgagta catctggaag ccggcgtcgt tgagctcgag ccccggcgtt
     4861 cgcagatcac cgccctgtgc ccagcgcaca tgcttcgtct cgcccgaacg accgacgaac
     4921 cacgacatgc cgaggcctga caggctcgtg cgcgtcggat cgagcatgac gttgctggca
     4981 tcgggccgct ggaacaggtg gcggttgagc tgctgggtat ttgcgatcgc tgcctcggtg
     5041 ccgtgcacgt agctgccgac gccgtgaacg tcgagctccc acgcattgtc gccccagcga
     5101 tgggtgaact gggcgcccgc ggtgtacgcc tgatcgtgat tgatatcgac gagcggagtg
     5161 ccgtcgagcg agcgattcac cgcggtcgcc gacgcaccga tcgtcgttct gccgccgtgt
     5221 agatcgcgct tcacgcgacc gaccgcgtag ttcgtgagcg gcgcgacgac cgggttcatc
     5281 ttcgtgccag cgtcgtcgac gatcgtcgcg ttctcctggc cggtgacggc gtcgagcacg
     5341 ccgaccgacc aaccgctgtt cgtcttaccg gtaaccttcg cggcgctgta gatcgtcgtc
     5401 gacgtcggcg cgtcgatgaa cgcgtagtcc atatcgggtt ccgccggcgc agcaccgatg
     5461 cggcggctat agaacgcgcc ctcgatcaag ttgtcgccgc tgccgattgg cagcttgaag
     5521 agatcgacgc cttcgaggaa gaacggccgc ttctcggcga agaacagctc gttcgcggac
     5581 aggttgacct gcgacggatc tgcttcgacc tggccgaaat cgggattgat cgttgccgat
     5641 aacgtgaacg ccggaccgag gccgaacttc acgtcgacgc cagcgttgcc gagtgctgaa
     5701 gtctcggggt tgagcggatc gcccgcgtcg atcggctggg tctccacgcc gccggtcgcg
     5761 tacggcaaga gctcgagccg gcgggccgac ttgttgacct cgatcccctc gacgatgccg
     5821 aaccgactga cgatctggtt gccggtgcgc ggccacggcg accacgcgct ctgctcttgg
     5881 ttgcgcgcga tcatccgcac gacctgcaga ccccattcgt gcgtctcgcc accggcgaaa
     5941 cgtagctggc tgagcgggat gcggacctcg gccgtccaac cttcgggcgt gatcgcgaca
     6001 tcgccggtcc acacggcgtc ccaggtgtcg tcctgtgcca tgtcatcgaa cagcatcatg
     6061 tcgcgctgta cgccggcggc gttgatctga aagacgtacg cggtgcgctt gtcgtgatac
     6121 gagtcgaatc cgatcgcgat cgcatcagcg agcgcatcga cgtcgcgacg cgtgagcgtg
     6181 cggcggatct tgcccggctc gggatcgctc gcccacacgc cgacgtagat cgcctcgtcg
     6241 tcgtagagca cggcgaactg cgtgtcgtgc gatgccttgg cgccgtcctt cggaaagcgc
     6301 tgcgtgaatc cgccctgccg cggcgcggat gcccatgcag cctcgtcgag cttgccgttg
     6361 atcgagatcg agcccgtgcg tcgggtcgcg tgcgcgcgca cctcggccgg cagcgtatcg
     6421 gcatacgcag ccgcgctcgt gaggacgagg aacaagccaa gacggcgcat gcgtctgaga
     6481 tgcgtggcga gcacgaacgg tggactcgcg gggcaacccg atgttgcatc aggagaattc
     6541 cgccgaatag tgcgacagcg ctgcgacagc acaaatcgtc gcgcttagcg gcgcaccttg
     6601 gacgttctct caggcatttg ctcgtcagag acgcgggcat gctccagcag taattgcgtg
     6661 atgctggtat tgagctcgtc ccgccctccg atcgagcacg gcgagaattc gaactgacca
     6721 gcgtgccagt cgagaagtgc catcagccgc tgcttgggcg cgccgtttcc ggtactcgcc
     6781 tcgaccttga gcaccttgcc ctccgacacg aacaggcgcg cgatatcgcc gtcgcgtagc
     6841 agcaacagga tgccgctctt gcgctcgaac tcgaacagcg agagcaacgt gccgagcccg
     6901 atgtcgacga ggttgccgcg caacccggga ttcgcgatcg gcgcgactgg cgccacgacg
     6961 cgatgcacgc gaatcacgag ctcctcgtcg agaaatggcc gcggaatgta gtcgcgaatg
     7021 ccggcgcgga atgcctcgag ccgcgcgaga tcgccctcgt cgccggtgag caccagcggc
     7081 acatcagcga gcacggtgtg ctccgacatc gcgtaggcga gatcgacgcc gctcataccg
     7141 tggtcgagca ccgcggcgct gatgatgacg tcgggacgcc actgcgtgca cgcgtcgagc
     7201 gcttcgggca tcgacgcgac cgcgatgatc ttgaagcccg cgccttccag gcaccgcacc
     7261 atgcgttcgc gaagagggcc ggtgggctcg acgatgatcg cggacgtcgt cgtcgtgagg
     7321 cccgggttcg tggtctcgac ccgcacgttg tcgacgtgcg cgcgcagctt gatccgcgat
     7381 ggtgtgtcga caccgatcag ctcgaagccc atccccggat gacgaccgag tgcacgcgcc
     7441 gccaccggcg tcagcatgtg cgcgacgcgc gcgcgcatcg cgaggaacgt cgagtcgggg
     7501 agcgtgagcc ggatctctgt ctcctcgccg accggaagca acgcgtcggt ccggatgaac
     7561 atgccgcgcg cggagagatc ttcagtctcg gcttcgacag ggccgctcgg tcggtcgaat
     7621 tcgactcggc agcgactgac cagtcgcagg tcgcgccccc gatgtttcgg caccgaaacc
     7681 taggataccg gtcaggcttt tggttgtccc gtttacggga gggaccgacg aagcggtcgg
     7741 tttcaacaag ttggcgctcc cgcttgcaga atccggaagt ttcaagaatc tttgaaacgt
     7801 cttgaagcat cggagaagca gcccatgacg gtcgcttacg aatcagtcac gtcggtcggg
     7861 gctgcggtcc tcgatcgact cgcaaacatc agcggtgcac gcggtgcaat cgatctgcgc
     7921 cagcgtcttg ctgctctcgg acgctgggtc cgcgcggatc tcgccgactt cgaggcggag
     7981 ctggcgacca tcccgcgcgg cgcacgcgtc gtccagcaag cagcacatca cctgctcgat
     8041 ctgcgcggca agcacctgcg gccgctgtgc gtcgcgctcg cgagccggtt cggcgaaggc
     8101 ttcacgccac gcgcgcgcgg cctcgcggtt gctgtcgagc tcgtccacac cgcgacgctg
     8161 ttgcacgacg atgtcgtcga cgtcgcggag cggcggcgcg gtcaaccggc cgcagcaatc
     8221 gtctacggca acgcagcttc gatcttcgcg ggcgactggc tgctcgtcgc agcgcttcgg
     8281 cgcatcagcg cgacgggcgt cgacggcgtg ctcgagaaga tgctcgaggt catcgacgag
     8341 atgatcatgg cggagtccct gcagctcgaa cgccggggcc gcgtcgtcgc cgatcgcgat
     8401 gagtacttcc gcatcgtcga gggcaagacc gccgcactgt tccgctgggc gatgctcgcg
     8461 ggcgcgcggt gcgccggcat ggcggccgag cacgaagccg tgctcgagaa atacggcgtg
     8521 cacctcggcg tcgcattcca ggctgtcgac gacgagctcg acttcgccgg cctcgaagca
     8581 accggcaagg atccactcgc cgatctgcgc gaaggcaaga tgacctatcc gctgatcgtg
     8641 gcgctcgagc gggaatcgtc gctgcgtgcg cggctcgaag ctgttctcgg tgaagacgtt
     8701 ccgagcccac tcgagctaac ggcgatcgcc gcggctgtgc gagcaacggg cgcgctgcaa
     8761 gcaacgcgcg agctcgccga acagcacgta cagctcgcac tcgagacgtt ggcagcgctg
     8821 cccgccggca ccgcacgcga atcgctcgag accgtcgcac tcgcttcgct ggagaggacg
     8881 tcatgagcca gcttgcccac caaccagagt cattgccgac gctcgccccg cagcagctcc
     8941 ccacgctcgg cagtggcgag atgttcgatc ggatcgcggc acgctacgac ttcgtcaatc
     9001 gcgtgctgtc gctcggtctc gaccagcgct ggcggcgacg cgtagtccgc tcgctcgggc
     9061 tcgagacgcg caagcggttc ggccgcaagg acgtgcgcgg ccagtggttc ggcgagcgcc
     9121 cgcgcgtgct cgacgtcgca accggcaccg gcgatctcgc gatcgaaatc gcgcgtgcgt
     9181 gccccggcgc gtccgtgatc ggcctcgacc cgtccggcgg catgctcggt gtcgcgaagc
     9241 agaagctcgc gaagcgcggc ctctccgatc gcgtggagct cgtgatcggc gacgcgcagc
     9301 accttccgca aaagaactgc gaaatcgatg cagcgacgat cgcgttcggt attcgcaacg
     9361 tgcccgaccg cggccaggcg ctgcgcgagc tcgcgcgcgt ggtgcgaccg ggcggccgga
     9421 ttgcagtgct cgagctcggc gaaccgcggc ggggcttgct tgcagccgct gcacgcctcc
     9481 actgccacca catcgtgccc aagctcggtg gctggctcag cggctcgcgc gagtacgagt
     9541 acctgcagcg ctcggtcgcc gcgttcccgc cggccgagga gttcgccgat ctgatgcgcg
     9601 cctccgggct cgacgtgatc gaggtcgtgc cgctcacgtt cggcgtctgc acgctgtatc
     9661 tcgcgacccc tgccgaggag cgctgacatg aaggcggtac ccgacatgtt catggagcga
     9721 atcggcgacg tcgcgcatgc cttcgcgctg cgcgacgccg acgacgacct cgatgcgggc
     9781 ctcgcaatcg cacgccggca agcaggctgg acgttcatcg cgctgccggc gccggtcgtc
     9841 gccgcgcctg gactcgtcgc tgcgtggcgg tcgacgccgg tcgtcgcgtg gtcgtcgtct
     9901 gcgctgacga tcgtcggcgt cggcaccgcg cacgagctgc gcggccgcgg cggtgcgcgc
     9961 tggcgcgaag tgatcagcgg tgctgcgcgc gtcgagcaca cgacgctcgt cgatcgcagc
    10021 ggcaccggtt cgctcgcgcg cccgcgctac ctcggtggcc tggcgttcgc gcccggcgcc
    10081 gccgacggtg cgccgtggca gggcttcggc
//
LOCUS       DASUFX010000662        11023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_245116,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000662 DASUFX010000000
VERSION     DASUFX010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245116"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFV99_26815"
     CDS             complement(<1..944)
                     /locus_tag="VFV99_26815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX5063027.1"
                     /translation="MRSPTSTVPASVLVVDDVPAQRLALREILSHADYRIEEAGSGQE
                     ALMKLSDSEFAVLLIDAVMPDMNGFKLAEKIRGEYERANVPILFVTAQPIDIDLVFRG
                     YRAGAVDYLVKPLDPEVVRAKVSVFVELYRQRKLIEQSLREKEILLREIHHRVKNNLQ
                     IVSSLLNLQSQRQPAEIQSAFLDSTARIRSIALVHENLYQMGNFATINEDAYLRALVT
                     SLQQTYGSTVEIEIEDHGVELDMDTAILCGLIVNELVSNALKHAFPDAQEGKIIVSLA
                     ELQSGNIVLEVRDNGVGLPREQRDASLGQTMGFQIVKSL"
     gene            complement(1023..1787)
                     /locus_tag="VFV99_26820"
     CDS             complement(1023..1787)
                     /locus_tag="VFV99_26820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063028.1"
                     /translation="MAVQPSIIRLYKVAGLVALTAILVGLLGFLIVNIFYFFDNSWVR
                     PVVLSPTHQKVVEASTQLADARLRASQLDTEKLEIEANLSELERSVAMDDKFLADVGT
                     AVDAPKTPDQWMVRREVDRAKLDKANQEGRKAPLEARLQSLGLRIKEQEAVVHRLEQS
                     PYLRAVNGKVVLAFVPYSNLRHIKAGTKLYGCSWGLVMCSRVGKIKATIDGEVQDIHP
                     HDQSVQRGVMVEIELSTPSAEGNSVLFAGGKPLWLF"
     gene            complement(1863..3155)
                     /locus_tag="VFV99_26825"
     CDS             complement(1863..3155)
                     /locus_tag="VFV99_26825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5063029.1"
                     /translation="MLALVVFINRYIAGVLIRVVRGKKWDETRDDFEPSVTVVIPMFN
                     EGEAIKETLASLLASEYPREKLEIVVVDDCSTDNSFELAREIARGSGGQLRVIRNRVN
                     LGKRRSINRAVAASDREIIVSVDSDVVVDPNAIKELTRRFTDDRIAAVGGWVDVRNKQ
                     ENWLTRMQTIKYWYGYFFLKNLEWGFRRIMCLSGCLTAYRRHVLVELEPVLEQRSILG
                     VSIKYGEDRFLTRQIIKAGYLTTMTHAARCRTFVPANLPEYFSQQLRWRRSNIVDYSG
                     AVSHVWKLNPFLAIHFFAQFALLTAYPITVIRALSSGWFFEALQIHLYVVAVFGLYYR
                     FRVRKLPASERVGTLAFLPIALLMPITYALMTPLAMFTLDSGSWETRGHEAETEAGTD
                     AIPESITSTGQHVLVPALAGERARSSRGTGSHAQLPAA"
     gene            3504..4538
                     /locus_tag="VFV99_26830"
     CDS             3504..4538
                     /locus_tag="VFV99_26830"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEX5063030.1"
                     /translation="MAGPEATKTPSHDPDGAVTAPPAASGSPWIDLAPAKILSSQSGD
                     DDPHDHRLATLLTLGGLHAGFWGWAYIAWYRNHPEKDKHEFGADGLFGVNTYAGGADK
                     LGHAWATMVLARGGTALLDAGGWKRTHAALISATMSELLFLGVEIKDYYYYEFSPGDF
                     AFNTIGALTAVALDLTPRLDELIDFRVEYWPSKQFRYNFTNPDSPCAKRTPGQPSCSR
                     LNIAEDYSGETYLLALHLGAFHPLRDMKYGTWSRFVDVVMGFESRNYKPPPTLLMDTE
                     AMVPRHQTLFLGVTFNAQGLIDYLLPKQSKLRKVGHGVLEVITPPWTRIVPSKIEGTR
                     STTDPNMGGA"
     gene            4628..5464
                     /locus_tag="VFV99_26835"
     CDS             4628..5464
                     /locus_tag="VFV99_26835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063031.1"
                     /translation="MRAVLVAALALAACHTFDPIREVPHDHREVATTTEAISLILAEN
                     PQPLVFAIGEYHQTRNAISSTSPLARFTQEIIGMLEPYADHLVVEAWLDANCWGKTNE
                     QVIAATQRSETTQMEVLRLMHRGQRLHLQTHTLPMTCIEYDAVIDASGHVDFLLLLEL
                     ITDKLTETTRSLLAKDRAVIVYGGALHNDLYPRWPLESLSYAKPLAHELGGKVLEIDL
                     VVPEIVAGMEMIRQEPWFPLLGRASPKRVLIWQRGPASYVVILPAQSEEVAKVAKLVD
                     PM"
     gene            5474..7078
                     /locus_tag="VFV99_26840"
     CDS             5474..7078
                     /locus_tag="VFV99_26840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063032.1"
                     /translation="MRAWYIAIFVAVFGLYLLSSSREPAWGDARPMWEVADRLVQHGA
                     IDIKTRWPDDMPPGSGGKTYGIAAIGTVLVHVPGVALAAVSHSLAPRHDTLLRPIFTH
                     LAPAALGALACLLFFGLLRDLGRSARTASLCTAILACATTVWIYARMPYGEIIQVACF
                     LGLFRYTLRVIAEPSKRNALWLGLWAGYLFNTKYVYATAIAGAALLILWMLRKQRADL
                     KRVVLWASATAGPLLVLGLVYNYLRFGSITHTGYEPYLSWYFGGSVFDGAWGMLASAN
                     KSALLYSPPLLLALLGWPRAIRENKTLGIALAVLVVPTFIVYCTYRSWSGDYAWGPRY
                     FVWTVPVLLVGMAWFVDNITSRWKLSFIAAVIAIGIVVQLLGAALYWDHFIRIAIDAK
                     NQWLGNPNRSGAYIPEKGRGHCDSCFEDTYEILWTPAFQPIKGHLWLLESLARGDADG
                     TEAQVDAPWRRYTSLPINLNASYPRVRVDWWGLLWLEDATNTWLVGVILLMLFAGTTA
                     FGIWMWIRLHRSGIPGSDAEGSAEST"
     gene            7050..7922
                     /locus_tag="VFV99_26845"
     CDS             7050..7922
                     /locus_tag="VFV99_26845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063033.1"
                     /translation="MLKAVPNPPDEPETRAPSDEDVLRWALAYLRHAPLSLTLREINR
                     LVALETVGKHDGPVLDVGCGDGFWWTMREDNREIYGIDISASEIAQAQKRIHAQLSDV
                     SRETPFRGTKFGEVIGNCSLEHVPDIDAALSNLRASAADNARLLMFVPTPGWAYQGRM
                     QSLLLKFSPRLAMAFSGALNGFFQHWHLYDHKVWRRLLEQHGWHVRAVYGLGSARSEF
                     LFRAFMPPAFLQFVAKSITGFYPAKLYRFLPDAAIAPLAKLVRWAVSDPIVPVDSPHA
                     YEYLIVAEAAPVER"
     gene            7937..8575
                     /locus_tag="VFV99_26850"
     CDS             7937..8575
                     /locus_tag="VFV99_26850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063034.1"
                     /translation="MNVQPDYFANHERARKFPWTLYHAPLERDLARFLERVAADHPSG
                     DILVVGCGLLHEIDAAPPGMRYTVADIDPRAVDAVLARKDPRIVSGRVVAPEHPIALG
                     KRFAGIYAKEVIEHIHAWPTWLAGLRRALVPGGRLWLSTPNYGEPWLPTLESTVLEMV
                     ARRDGFSRRHIHPTRFSRRSLARGLSAAGFVDVHAHVMPTRLAITAWAQAPG"
     gene            complement(8605..9369)
                     /locus_tag="VFV99_26855"
     CDS             complement(8605..9369)
                     /locus_tag="VFV99_26855"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX5063035.1"
                     /translation="MGATWIALIAFYVVFASIVEEAPHTFRYRGTAELTKVEEITYDG
                     KTRYIANYDYTGANGARHSNRSMSDERPPLGPHAYGYDEDGGSTELVGMRPAPDDPGL
                     GTLLLTSLGFLLAAFELHSAIRMIRRLRRSHTVHADVKSATRTDSGHKRGHGVTKLVL
                     EYEDRDGARHEVSLARDDSPVLEDAKSLHLLVEPDDPQRFLTRFDLPPNSMTGAGKLV
                     GHWTAWFVVVLPAVAILLFAAAVYFVAAGPMPVFKI"
     gene            complement(9439..10035)
                     /locus_tag="VFV99_26860"
     CDS             complement(9439..10035)
                     /locus_tag="VFV99_26860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063036.1"
                     /translation="MAGARRTLRAVFDRGQMQFGWLWVAGVIWVAVWVLPDGRWNRTF
                     DGHAIATVERIESDGADPPDLHIFFVFHANGREYHNYGIDEGAPRDLVAGRELDVAYV
                     SSDPTYAELRVPGVRTRFSSATFELAILGLFAGAGVVIMLFDLVTGIRERRRPVESDA
                     TEIAERTRERIWYLLVLPAAAFCGAVTLIVLYLTPSTP"
     gene            complement(10035..10847)
                     /locus_tag="VFV99_26865"
     CDS             complement(10035..10847)
                     /locus_tag="VFV99_26865"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX5063037.1"
                     /translation="MKTVMIISDATGETAERMVRAATLQFSEPVQIRLFSRVRLESEL
                     EQILEKAAELKALVVFTVVNQEERDLISKLVEKHSIEIVDLMGALISKLALYLGAPPA
                     GVPGLLHMITDDYFRRIEAVEFAVKNDDGAEPRNLPKADLVLVGISRTSKTPLSTYLA
                     QRGLKVANVPLVLGVDPPEELSQVDDRKVFGLIVQPDMLMRIRQARLSHLGMPQDSSY
                     GQRAHIDSEIAYSREIFRKHANWPVIDVTNRAIEETAADILRIYTERNAMPS"
BASE COUNT         1887 a         3599 c         3541 g         1996 t
ORIGIN      
        1 aggctcttca cgatctgaaa ccccattgtc tgccccagcg atgcatcgcg ttgctcgcgc
       61 ggtaacccga ccccgttgtc acgcacctcg agcacgatgt taccgctctg gagctcggca
      121 aggctgacga tgatcttgcc ctcttgcgcg tcggggaacg cgtgcttgag tgcgttcgac
      181 accagctcgt tgacgatcag cccgcacagg atcgcggtat ccatgtcgag ctcgacgcca
      241 tgatcttcga tctcgatctc gacggtcgaa ccataggtct gctgcaagct ggtcacgagc
      301 gcgcgcaggt aggcgtcctc gttgatcgtg gcgaagtttc ccatctgata aaggttctcg
      361 tggacgagcg cgatcgaacg gatgcgcgct gtactgtcga gaaacgccga ctggatctcg
      421 gcaggctgcc gctggctctg taggttcaac aggctcgaca cgatctgcag gttgttcttc
      481 acgcgatggt gaatctcgcg aagcaggatc tctttctcgc gcagcgactg ttcgatcagc
      541 ttgcgctggc gatacagctc gacgaacacg ctgaccttcg cgcgcacgac ctccggatcg
      601 agcggcttca ccaggtagtc cacggcgccg gcgcgatagc cgcgaaacac gagatcgatg
      661 tcgatcggct gcgccgtcac gaacaggatc ggcacgttcg cgcgctcgta ttcgcctcga
      721 atcttctcgg cgagcttgaa gccgttcatg tcgggcatga cggcgtcgat gagcagtact
      781 gcgaactcgg agtccgacag cttcatcaga gcttcttgcc ccgaccccgc ttcctcgatc
      841 cgatagtcgg cgtgcgacag aatctctcgc agcgcgagtc gctgcgcagg cacgtcgtcg
      901 acgacaagca cgctagccgg aacggtgctg gtcggagaac gcatgggctg ggccctcgac
      961 caagcgagcg tgcaagccgc gtacgcgacc gctcgccatg tcgcagcact acgtagcgct
     1021 actcagaaca gccagagcgg cttgccgcct gcgaacagca cgctgttgcc ttcagccgac
     1081 ggcgtcgaca gctcgatctc gaccatcaca ccgcgctgca cgctctgatc gtgcgggtgg
     1141 atgtcctgca cctcgccgtc gatcgtcgct ttgatcttgc cgacgcgcga gcacatcacg
     1201 agaccccacg agcaaccgta gagcttggtg cccgccttga tgtggcggag gttcgagtac
     1261 ggcacgaacg cgagcacgac cttgccattg accgcacgca gatacggcga ctgctcgagg
     1321 cgatgcacga ccgcctcctg ctctttgatg cgcaggccga ggctctgcag gcgcgcttcg
     1381 agcggcgcct tgcgaccttc ctggttcgcc ttgtcgagct tcgcgcggtc gacctcgcgc
     1441 cggaccatcc attgatcggg cgtcttcggt gcgtctacgg ccgtaccgac gtcggcgagg
     1501 aacttgtcgt ccatcgcgac cgaacgctcg agctcgctga ggttcgcttc gatctctagc
     1561 ttctcggtgt cgagctggct cgcgcgcagg cgcgcgtcgg cgagctgggt cgatgcctcg
     1621 accaccttct ggtgcgtcgg cgacaacacg accgggcgca cccaggagtt atcgaagaag
     1681 tagaaaatgt tgacgatgag gaagccgagg aggccgacca agatcgctgt cagagcaacc
     1741 aggcccgcca ccttgtagag ccggatgatc gacggctgaa ccgccaccca aaatcgaggg
     1801 gtcttccgag cttccatggc gcccatatgc ggtgaggctt actccaagaa tcaggccgct
     1861 gtctacgcag caggaagttg ggcatgggag cccgtaccgc ggctactccg cgcacgctcg
     1921 ccggcaagtg ctggcaccaa cacgtgttgt cccgtgctgg tgatgctctc cgggatcgca
     1981 tccgtgccgg cctctgtctc cgcctcgtgg ccacgcgtct cccagcttcc cgagtcgagc
     2041 gtgaacatcg cgagcggcgt catcagtgcg taggtgatcg gcatgagcag cgcgatcggt
     2101 aggaacgcga gcgtaccgac gcgctccgac gccggcagct tgcggacgcg gaagcgatag
     2161 tacaggccga acaccgcgac gacgtacagg tggatctgca gcgcctcgaa gaaccagccc
     2221 gagctcagcg cccggatcac ggtgatcgga tacgcggtga gcagcgcgaa ctgcgcgaag
     2281 aagtgaatcg caaggaacgg gttcagcttc cagacgtggc tgaccgcgcc cgagtagtcg
     2341 acgatgttcg atcggcgcca gcgcaactgc tgcgagaagt actcgggcag gttcgcgggc
     2401 acgaacgtgc ggcagcgcgc cgcatgcgtc atcgtcgtga gatagccggc cttgatgatc
     2461 tggcgggtga ggaagcgatc ctcgccgtac ttgatcgaga cgccgaggat cgagcgctgc
     2521 tcgagcaccg gctcgagctc gacgagcacg tggcggcggt acgcggtgag gcagcccgac
     2581 aggcacatga tgcgcctaaa gccccattcg aggttcttca ggaagaagta cccgtaccag
     2641 tacttgatgg tctgcatccg cgtcagccag ttctcttgct tgttgcggac gtcgacccaa
     2701 ccaccgaccg ctgcaatgcg gtcgtcagtg aaccggcggg tgagctcttt gatcgcgttc
     2761 gggtcgacca ccacgtcgga gtcgaccgag acgatgatct cgcggtccga ggcggcaacc
     2821 gcgcggttga tcgagcggcg cttaccgagg ttcacccggt tccggatgac ccggagctga
     2881 ccacccgagc cacgggcgat ttcgcgagca agctcgaagc tgttgtccgt cgagcagtcg
     2941 tcgaccacga cgatctcgag cttctcgcgg gggtattcgg aagcgagcag gctggcgagc
     3001 gtctctttga tcgcctcgcc ttcgttgaac atcggaatga cgaccgtgac gctcggctcg
     3061 aaatcgtcgc gggtctcgtc ccacttcttg ccgcgcacga cgcggatcaa gacgccggcg
     3121 atgtaccggt tgatgaagac cacgagcgcc agcatcttca ccagcagcca aaggacgtgg
     3181 gtcgtcatcc tcggcccccg aatgcgaggc gcacgccaat cacggaatga caggtgaagc
     3241 gagtattcac ggtgaagcta gaaggaaagg ccgtgaatca aatagtcacg gctaagatca
     3301 aggagatgcg agcaaactat ctggtctagc gtcgcaactc aagttggttg acgcatgttg
     3361 ccacatcagg cgtgaccgat acacctgaac atcagtttgc tgagggtggc gcgcttgttt
     3421 ttgcacaacg tcggtgggat aggttgccgg tgcgcccggg cttcatcgcc ctgataattg
     3481 tcacgtggtt gcgtagcgtc gcagtggctg gaccagaagc cactaagacg ccgtcccatg
     3541 atccagatgg cgcagtcacg gcgcctcccg ctgcgagcgg ctcgccatgg atcgatcttg
     3601 ctcccgcaaa aatactctcg tcacaatctg gtgacgatga tccgcacgat catcggctcg
     3661 cgacgctact cacgctcggc gggctccacg caggcttctg gggctgggcg tatatcgctt
     3721 ggtatcgcaa ccatcccgag aaggacaaac atgagttcgg tgccgacgga ttgttcggcg
     3781 tcaacaccta cgcgggaggt gccgacaagc tcggtcacgc gtgggcgacg atggtcctcg
     3841 cgcgcggcgg cactgcgcta ctcgacgcag gaggctggaa gcgcacgcac gcggcgctga
     3901 tctccgcgac gatgtccgag ctgctgtttc tcggcgtcga gatcaaggac tactactact
     3961 acgagttctc gccgggcgac ttcgcgttca acacgatcgg tgcgctgacc gccgtcgcgc
     4021 tcgatctgac gccgcgtctc gacgagctca tcgactttcg cgtcgagtac tggccgagca
     4081 agcaattccg atacaacttc accaaccccg actcgccgtg cgcgaagcgt acgccgggcc
     4141 agccgagctg ttcgcgcttg aacatcgccg aggactactc cggcgagacg tacctgctcg
     4201 cgctccacct cggagcgttc cacccgctgc gcgatatgaa gtacggcacg tggtcgcggt
     4261 tcgtcgacgt cgtcatgggc ttcgagagcc gcaactacaa gccaccgccg acgctcctca
     4321 tggacacgga ggccatggtt ccgcgccacc agaccctgtt cctcggcgtc acgttcaacg
     4381 cgcagggcct gatcgactac ctgttgccga agcagtcgaa gctgcgcaag gtcgggcatg
     4441 gtgtgctcga agtgatcacg ccgccctgga cccgcatcgt gccgtcgaag atcgagggta
     4501 cgcggtcgac gaccgacccc aacatgggcg gcgcatagtc aggcttcggg ctacgggctt
     4561 cgggcttcag gttcggagct cgagctgact gctcgcaacc gatgcgtggc tcgcgtgtct
     4621 cgtcgacatg cgcgcggttc tcgtggcggc cctcgccttg gccgcgtgtc atacgttcga
     4681 cccgatccgc gaggtacctc acgatcaccg cgaggtcgcg acgacaaccg aggcgatctc
     4741 gctgatcctc gccgagaacc cacagccgct cgtgttcgcg atcggtgagt accaccagac
     4801 gcgtaacgcg atctcatcga cgtcgccgct cgcgcggttc acgcaggaga tcatcggcat
     4861 gctcgagccg tacgccgatc acctcgtcgt cgaggcgtgg ctcgacgcga attgctgggg
     4921 caaaacgaac gagcaggtga tcgcggcgac ccagcgcagc gagacgacgc agatggaggt
     4981 gctgcggctc atgcaccgcg gccagcgctt gcacctgcag acgcacacgc tgccgatgac
     5041 gtgcatcgag tacgacgcgg tcatcgatgc gagcggtcac gtcgacttct tgctgctgct
     5101 cgagctgatc accgacaagc tcaccgagac gacgcgcagc ctgctcgcga aggaccgcgc
     5161 agtgatcgtc tacggcggcg cgctccacaa tgacctctac ccacggtggc cgctcgagtc
     5221 gctgtcgtat gccaagccgc tcgcccacga gctcggtggc aaggtgctcg agatcgacct
     5281 cgtcgtcccc gagatcgtcg caggcatgga gatgatccgg caggagccgt ggttcccgct
     5341 gctcggccgc gcctccccca agcgcgtgct gatctggcag cgcggcccgg caagctacgt
     5401 cgtgatcctg cccgcccaga gcgaagaggt cgcgaaggtg gcgaagctcg tcgatccgat
     5461 gtaactcagg gagatgcggg cctggtacat cgcgatcttc gtcgcggtgt ttggcttgta
     5521 tctactgtcg tcgagccgcg agccggcgtg gggcgatgcg cggccgatgt gggaagtcgc
     5581 cgatcggctc gtgcagcacg gcgcgatcga catcaagacg cgctggcccg acgacatgcc
     5641 gccgggctcg ggcggcaaga cctacggcat cgccgcgatc ggcaccgtac tcgttcacgt
     5701 gccgggcgtc gcgctcgcgg cggtctcgca ctcgctggcg ccgcgccacg acacgctgct
     5761 gcggccgatc ttcacgcacc tcgcgcccgc cgcgctcggc gcactcgcgt gcctgctgtt
     5821 cttcggcttg ctgcgcgacc tcggccgcag cgcacgcacg gcgagcctgt gcaccgcgat
     5881 cctcgcgtgt gcgacgacgg tctggatcta cgcgcgcatg ccgtacggcg agatcatcca
     5941 ggtcgcgtgc ttccttggtc tgttccgcta cacgctgcgt gtcattgccg aaccgagcaa
     6001 acgcaatgcg ctctggctcg gcctgtgggc cggctacctc ttcaatacga agtacgtcta
     6061 cgcgaccgcg atcgccggtg cggcgctgtt gatcctgtgg atgctgcgca agcagcgcgc
     6121 cgatctcaaa cgcgtcgtgc tgtgggcgag cgcgaccgcc gggccgctgc tcgtgctcgg
     6181 cctcgtctac aactatctgc gcttcggctc gatcacgcac accggctacg agccgtatct
     6241 gtcgtggtac ttcggcggca gcgtcttcga cggcgcgtgg ggcatgctcg cgtcggcgaa
     6301 caagagtgcg ctgctctact cgccgccgct cttgctcgca ctgctcggct ggccgcgcgc
     6361 gattcgcgag aacaagacgc tcggcatcgc gctcgcggtg ctcgtcgtgc cgacgttcat
     6421 cgtttactgc acctatcgct cgtggagcgg cgactatgcg tggggtccgc gctacttcgt
     6481 gtggaccgtg ccggtgttgc tcgtcggcat ggcgtggttc gtcgacaaca tcacgtcgcg
     6541 ctggaaactg tcgttcatcg ccgcggtgat cgcgatcggt atcgtcgtcc agctgctcgg
     6601 cgcggcgctg tactgggatc acttcattcg catcgcgatc gacgcgaaga accagtggct
     6661 cggcaatccg aaccgctccg gcgcctacat cccggagaag ggccgcggtc actgcgactc
     6721 gtgcttcgag gacacctacg agatcctctg gacccccgcg ttccagccga tcaagggcca
     6781 cctgtggttg ctcgaatcgc tcgcgcgcgg cgatgccgat ggcaccgaag cacaagtcga
     6841 tgcgccctgg cgccgctaca cctcgctgcc gatcaatctc aacgcgagct acccgcgggt
     6901 tcgcgtcgat tggtgggggc tgctctggct cgaggacgcc acaaatacct ggctcgtcgg
     6961 cgtgatcctg ctgatgttat tcgccggaac gacggcgttc gggatctgga tgtggattcg
     7021 cctccaccga tcaggtatcc ctggctccga tgctgaaggc agtgccgaat ccacctgacg
     7081 agcccgagac gcgcgcgccg agcgacgagg atgttctgcg ctgggcgctc gcctatctgc
     7141 ggcacgctcc gctgtccctc acgctgcgcg agatcaaccg actcgtcgcg ctcgagaccg
     7201 ttggcaagca cgacggtcca gtgctcgacg tcggctgcgg cgacggtttc tggtggacga
     7261 tgcgcgagga caatcgcgag atctacggca tcgacatctc ggcctccgag atcgcgcagg
     7321 cgcagaagcg catccacgcg cagctgtcgg acgtctcgcg cgagacgccg ttccgcggca
     7381 ccaagttcgg cgaggtgatc ggcaactgct cgctcgagca cgtgcccgac atagatgccg
     7441 cgctgtccaa cctccgcgcg tcagccgccg acaacgcgcg cctgctcatg ttcgtgccga
     7501 cgcccggctg ggcctaccaa ggccgcatgc agtcgctgct actcaaattc tcgccgcgtc
     7561 tcgcgatggc attctcgggt gcgctcaacg gcttcttcca gcactggcac ctctacgatc
     7621 acaaggtgtg gcgccgcttg ctcgagcagc atggctggca cgtgcgcgcc gtctatggcc
     7681 tcggctcggc acgctcggag ttcttgttcc gcgcgttcat gccgcccgct ttcttacagt
     7741 tcgtggcgaa gagcatcacc ggcttctatc cggccaagct ctatcgcttc ctgcccgatg
     7801 ccgcgatcgc accgctcgcg aagctcgtgc gctgggccgt ctccgatccg atcgtgccgg
     7861 tcgactcgcc gcacgcgtac gagtacctga tcgtcgccga agccgccccg gtcgagcgct
     7921 gaccatggtc gcggcgatga acgtgcagcc cgattacttc gcgaaccacg agcgtgcgcg
     7981 caagttcccg tggacgctct accacgcacc gctcgagcgt gacctcgcac gctttctcga
     8041 gcgcgtcgca gccgatcatc cgagcggcga tattctcgtc gtcggctgcg gcctgctcca
     8101 cgagatcgat gccgcaccgc cgggcatgcg ctacacggtc gccgatatcg atccgcgcgc
     8161 ggtcgatgcc gtgctcgcgc gcaaagatcc tcgcatcgtg tccggccgcg tcgttgctcc
     8221 cgagcatccg atcgcactcg gcaagcgctt cgccggcatt tacgcgaagg aagtgatcga
     8281 gcacattcac gcgtggccga cgtggctcgc cggcctgcgc cgcgcactcg tgccgggtgg
     8341 acggctgtgg ctgtcgacgc cgaactacgg cgagccgtgg ctgccgacgc tcgagtcgac
     8401 ggtgctcgag atggttgcgc gccgcgacgg cttctcgcgg cgccacattc atccgacgcg
     8461 gttctcgcgg cgttcactcg cccgcggcct cagcgcggct ggtttcgtcg acgtccacgc
     8521 gcacgtcatg ccgacgcgcc tcgcgatcac cgcgtgggcg caagcgccgg gctagcgcat
     8581 cacgcgcttg ccgagcggtc tgtttcagat cttgaacacg ggcatcgggc cggctgccac
     8641 gaaatagacc gctgcggcaa acaacaggat cgcgacagcc gggaggacca cgacgaacca
     8701 agcggtccag tggccgacga gcttgccggc gccggtcatc gagttgggcg ggaggtcgaa
     8761 tcgggtcaga aatcgctgag ggtcgtctgg ctcgacgagc aggtgcagcg acttggcgtc
     8821 ctccagcact ggcgagtcgt cgcgtgcgag tgacacttcg tgccgcgcgc cgtcgcggtc
     8881 ctcgtactcg aggacgagct tcgtgacccc gtgcccccgt ttgtgaccgc tgtccgtccg
     8941 cgttgccgac ttcacgtccg cgtgcacggt atgcgagcga cgcagccggc gaatcatgcg
     9001 gatcgcgctg tgaagctcga atgccgcaag caggaaaccg agcgaggtga gcagtagtgt
     9061 cccgagccct ggatcatccg gtgcggggcg catccccacg agctccgtcg atccgccgtc
     9121 ctcgtcgtaa ccgtacgcgt gagggccgag tggcggtcgc tcgtcgctca tcgaacggtt
     9181 gctatggcgc gcgccgttgg cgcccgtgta gtcgtagttg gcgatgtaac gagtcttgcc
     9241 gtcgtacgtg atctcctcga ccttcgtgag ctcggcagta cctcgataac ggaacgtgtg
     9301 cggcgcctcc tcgacgatgg acgcgaagac gacgtaaaac gcgatcagcg cgatccaggt
     9361 cgctcccatc ctggtcgaac ggccaccgaa taggaggcgg gccgccagcg atcgaggcaa
     9421 agccgccacg cgtcgatgct aaggcgtcga cggggtgagg tacagcacga tcagcgtcac
     9481 ggcgccgcaa aatgctgcgg ccggtaacac gagtaggtac cagatccgct cgcgcgtccg
     9541 ctccgcgatc tcggtcgcgt cggactcgac gggacggcgg cgttcgcgga tgccggtaac
     9601 caggtcgaac agcatgatca cgacgccagc acccgcgaac aagccgagga tggcgagctc
     9661 gaacgtcgca gacgagaagc gcgttcgtac gccgggtacg cgcagctcgg cgtaggttgg
     9721 atcgctcgat acgtaggcga cgtcgagctc gcggcccgcg acgagatcgc gcggcgcgcc
     9781 ttcgtcgatg ccgtagttgt ggtactcgcg gccgttcgcg tggaagacga agaagatgtg
     9841 gaggtcgggc gggtcggcgc cgtcggactc gatgcgctcg acagtggcga tcgcgtggcc
     9901 gtcgaacgtg cggttccagc ggccatcggg gaggacccag acggcaaccc agatcacgcc
     9961 tgcgacccac aaccagccga actgcatctg gccacggtcg aatactgccc gcaacgtgcg
    10021 gcgggcgccc gccatcagct cggcatcgcg ttgcgctcgg tatagatgcg caggatgtcg
    10081 gccgcggtct cctcgatcgc gcggttcgtg acgtcgatga ccggccagtt cgcgtgcttg
    10141 cggaagatct cgcgcgagta ggcgatctcg ctgtcgatgt gcgcgcgctg gccgtagctc
    10201 gaatcctgcg gcatgccgag gtgcgagagg cgggcttggc ggatgcgcat cagcatgtcg
    10261 ggctggacga tcaggccgaa gactttgcgg tcgtcgactt gtgagagctc ctcgggagga
    10321 tcgacgccga gcaccagcgg cacgttcgcg accttgaggc cacgttgcgc gaggtacgtc
    10381 gagagcggcg tcttcgaggt acgcgagatg ccaacgagga cgagatcggc cttcggcagg
    10441 ttccgcggct cggccccgtc gtcgttcttg accgcgaact cgacggcctc gatgcggcgg
    10501 aagtagtcgt ccgtgatcat gtggagcagg ccggggacgc cggccggcgg agcgccgagg
    10561 tagagcgcga gcttggagat caacgcgccc atgaggtcga cgatctcgat gctgtgcttc
    10621 tcgaccagtt tcgaaatcag gtcgcgctct tcctgattga cgacggtgaa tacgacgagc
    10681 gccttgagct cggccgcctt ctcgaggatc tgttcgagct cgctctcgag gcggacgcgg
    10741 gagaacaggc gaatctggac cggctcgctg aactgcagcg ttgcggcgcg caccatccgc
    10801 tcggcggttt cgccggtggc gtcgctgatg atcatcaccg tcttcatctg gcggcctcta
    10861 tatcacgaga ctctgaacag ctgcgcgaac cgctcgatct ctcctgcgtc gagccacacg
    10921 ccgtgctggc tgcagtaatc gaatcggagc tcttcgcgaa acccgacctt catctcacgt
    10981 tgacaaattg gacatagagc cgcgccccag ggccagttcg tgg
//
LOCUS       DASUFX010000663         7670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_940094,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000663 DASUFX010000000
VERSION     DASUFX010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940094"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VFV99_26870"
     CDS             complement(<1..1324)
                     /locus_tag="VFV99_26870"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5063038.1"
                     /translation="MRRLWIGFALVLILSFAVLGWVGGRIYQEAPPLPDRVVTTDGRT
                     VFDRTDIQVGQDVWRSLGGMELGSIWGHGSYVAPDWTADWLHRELRLVLDEAALRATQ
                     VPYVALPAPQQAALREMLRREYRTNTYDPATKQITVSADRGRAIDTLVAYYAQLFSEG
                     RTAYALPAHTIENPARARALSAFFFWSAWAAATNRPGDTITYTSNWPHEPLIANEPTP
                     DTIVWTGVSIIVLLAGIGGLGIWYAVRKREEGPPELPETDPLLGTAPTPSQRATLKYF
                     WVVCALIVLQIVVGVITAHYAVEGAGFYGIPLAKVLPYAVSRTWHVQLGIFWIATAWL
                     AAGLAIAPAVGEEPRWPRLLVNVLFCALLVVVAGSLVGELLTVNGKLGDHGVWWFGHT
                     GYEYIDLGRAFQIALLIGLFLWVFLVARALRPALKRKDAQRPLLLLLLI"
     gene            complement(1442..1876)
                     /locus_tag="VFV99_26875"
     CDS             complement(1442..1876)
                     /locus_tag="VFV99_26875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063039.1"
                     /translation="MTQLIVVAIGAALFALWLASVIRSWRGSLRAKRRAARAGAGEDA
                     AALLLADAGFTIVERQARVVWAPLVDGEPLAMELRADYLVERSGELLVAEVKTGEEAP
                     SLETAATRRQLLEYHVAFAADGVLLVCPERGTIHRVVFPHRL"
     gene            1919..2434
                     /locus_tag="VFV99_26880"
     CDS             1919..2434
                     /locus_tag="VFV99_26880"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX5063040.1"
                     /translation="MRVELVYGDITTLDPAVDVIVNAANETLLGGGGVDGAIHRAAGP
                     ELRWECAKLDGCDTGDAKVTKAYRLPAKHIIHTVGPVWEGGTIGEAALLASCYRKSLQ
                     LAAGLGATSIAFPAISTGVYGYPLRDAAEISIKTILAHPFAQPERIVLCTFDPETTEA
                     TREMLAALTAY"
     gene            2444..3010
                     /locus_tag="VFV99_26885"
     CDS             2444..3010
                     /locus_tag="VFV99_26885"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX5063041.1"
                     /translation="MQIEILQGDITKLDVDAIVNAANSSLLGGGGVDGAIHRAAGPDL
                     LEECILIRNRDGGCPTGDAKITKGYRLPAKHVIHTVGPVWNGGSRGEPELLASCYRKS
                     LLLAAEAGAKSIAFPAISTGIYGYPFRPAAEIAVRTIRAHTGELPERVILCTFDEHAT
                     EITHEVLAASDSASAPAPTEQADEGEAE"
     gene            complement(2957..4168)
                     /locus_tag="VFV99_26890"
     CDS             complement(2957..4168)
                     /locus_tag="VFV99_26890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063042.1"
                     /translation="MRLVAGLLLVTACASEPTLGEDRAELTAAVRHERYGLIRDSAAE
                     MGLYNAALMAGIAISETNLAHCQSEATYACQGPASPSCNGGPIIAGAADGPCADMQGG
                     LGMFQFDAGTYAQTVATYGDSILTIEGNTAQAVNFVVERSQTSIAGIDDWLGAVGWMN
                     GVPMQAGDPKTEEWASFLACRYNGCCTTSATCQSRAAGYRDNAIMAFNEMGADFWRTS
                     DRCAAIPQGGVIDQRSECYLAAGDPRYWHRETGGYDDDREWTGTTSNAAAANFALWIV
                     KTSRASRFSVEVNLDGGTFGTSKQAKYVIVHAGATDEVVVDQTSASGWVSLGEFDFAG
                     TGDEHIMLADNTGEAGATDTTLLFDAVRVLSPDDPGDGGGCCDASGRGGPTALLGFAF
                     VGLLGRRRRAC"
     gene            4252..5376
                     /locus_tag="VFV99_26895"
     CDS             4252..5376
                     /locus_tag="VFV99_26895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063043.1"
                     /translation="MVPLRFLLVILVALPGCPYCDFDTPPLDTDVTREKALDEAFHLD
                     PRWLGGDAAYTIDLGDDTSLWLFGDSFIATSPNFTRSESTMVRNSVALMGGRDLATAS
                     MQFAWRDGAPPTSFFPEDADHWFWPNGGVRVPNGPLLVFLSELRATPGEGLGFAGVGM
                     RALRVADPSGSPLDWTLEPTTVHSPAFAPTMNVSCSTVVDDKYLIAVFTGDGNHDGML
                     ARWPLTAIANGDDLGNPEWWTGTIWLRESFLTKLPKVVIPDGGSECSLHYDTIGSAGW
                     VYTWSRGFGDTTIAMRTAWELTGPWSDPFDIFTPPESKVDNAFVYAAKAHPALIGPNG
                     GLIITFADNSFTFADLFDAAKAATLYWPHVVEVLIYQIVC"
     gene            complement(5373..6254)
                     /locus_tag="VFV99_26900"
     CDS             complement(5373..6254)
                     /locus_tag="VFV99_26900"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX5063044.1"
                     /translation="MKRLLLLATLLAPSLAHADDEVAPTTDAPAAATTDAPAAEPPVA
                     PAKPKRKRFYVRAGVAHIAPLEQSREMELSDVDGPASLALMNGPIAGSGASVSSATIP
                     GIIVGYVLPVGNDKLSVETILGLPFTVTFHATGTLATDSLAPTALGLPTGVPPLGSDL
                     GTAKAVPIVATLVYQVAQLGKVTPYVGAGPSIMFATNEKVTNMQLTAVAEPQMSIAPA
                     PGLVLQAGIDAMITKRIYARLDVKFIAFMLARATVEHVQVKTPDLPLFDNVEVGTAKM
                     SMWLNPLVLQAGIGTDF"
     gene            complement(6251..>7670)
                     /locus_tag="VFV99_26905"
     CDS             complement(6251..>7670)
                     /locus_tag="VFV99_26905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5063045.1"
                     /translation="DTVAGVVATTIESHGAAVVVRPLAPLAYSKIYRVVFNDVADVAG
                     NKLTMNNLSFVTPTLLSTNVSPTVSAVHPGVACALTGATANSPGRCAGGASSDDLYKP
                     FTLGADEPIAVEFTAPIRRNQVVRGTACNQGDVRIEKVDMAGTCTGVVPGTLLTHDRS
                     LSFIPDQPWTPGERYRLTLISGGNDTCDAGELCGTSTAANFDPLAGANGGDGGGPALA
                     VTYVGAEKSGGTFLMTSPFPFTDVNGSGVRDTSEQLRDDNSAAMRITGTGGSVNSASF
                     DDPDCVPSTPEKENCMYLVGSMPAVMGDVTTTCPLPGGESAPSCVPVTLSAQAMYGTS
                     ISMTANLPVVGGLSTPTGVQVMRIREPAGGPVMGYIIDRSGTPTMIVALDLYMDAPDM
                     SLPLGATHDLHSKKLSVILEGPLEFLPDGRIAISLANRADLAITVNISPPLIGDGSIN
                     LLVPKGMMKLKLVSPPERGVSL"
BASE COUNT         1289 a         2643 c         2498 g         1240 t
ORIGIN      
        1 agatcaacag gagcagcagc agcggtcgtt gcgcgtcctt tcgcttcagc gccggacgca
       61 gcgcccgcgc gacgaggaac acccagagga acaatccaat caacagcgcg atctgaaatg
      121 cccgaccgag gtcgatgtac tcgtagccgg tatgaccgaa ccaccacacg ccgtgatcgc
      181 cgagcttgcc gttcacggtg agtagctcgc cgacgagcga gccggcgacg acgacgagca
      241 gcgcgcagaa cagcacgttc acgagcaacc tcggccagcg cggctcctct ccgaccgccg
      301 gcgcgatcgc gagcccggcc gcgagccacg cggtggcgat ccagaagatg ccgagctgga
      361 cgtgccacgt gcgcgagacg gcgtacggca gcaccttcgc gagcggaatg ccgtagaagc
      421 cggcaccctc cacggcgtag tgcgcggtga tcacgccgac cacgatctgc aagacgatca
      481 acgcgcacac cacccagaag tacttgagcg tcgcgcgctg cgatggcgtc ggggccgtcc
      541 cgagcaacgg atcggtctcc ggcagctccg gtggtccttc ctcgcgcttt cgcaccgcgt
      601 accagatgcc gaggccgccg atgccggcga gcagcacgat gatgctgacg ccggtccaca
      661 cgatcgtgtc aggcgtgggt tcgttggcga tcagcggttc gtgcggccag ttgctcgtgt
      721 acgtgatcgt gtcgcccggc cgattcgttg ccgccgccca ggcgctccag aagaagaacg
      781 cggacagcgc ccgtgcgcgc gcgggattct cgatcgtgtg cgcgggcagc gcgtacgctg
      841 tgcgcccctc ggaaaacagc tgcgcgtagt aggccacgag tgtatcgatc gcgcggccgc
      901 gatccgccga caccgtgatc tgctttgtcg ccgggtcgta cgtgttggtg cggtactcgc
      961 ggcgcagcat ctcgcgcaat gcggcctgct gcggcgccgg cagcgcgacg tacgggacct
     1021 gcgtcgcgcg cagcgccgcc tcgtcgagca cgaggcgcag ctcgcgatgt aaccagtcgg
     1081 ccgtccagtc cggcgcgacg tacgagccgt ggccccagat cgagccgagc tccatgccgc
     1141 cgagcgagcg ccacacgtcc tggcccacct ggatatcggt gcgatcgaac accgtgcgcc
     1201 catcggtcgt cacgacgcga tcgggcaatg gcggcgcctc ctggtagatc cgcccgccga
     1261 cccagccgag cacagcaaac gacagaatca gaacgagagc gaatccgatc caaagacgcc
     1321 tcatggcgag ggatcgatgc agcgaacgtt cccgaggccg cgttgcggtt cgcgcagttc
     1381 gcgcctgcgc agcgtgcgtc gacgcgcagc atccctgtag cgcgcccgct ccgaagactt
     1441 gctacaggcg atgggggaac acgacgcgat ggatcgtccc gcgctcggga cacacgagca
     1501 gcacgccgtc cgccgcgaac gcgacgtgat actcgagcag ctggcggcgc gtcgccgcgg
     1561 tctcgagaga tggcgcttcc tcgccggtct tgacctcggc gacgagcagc tcaccgctgc
     1621 gctcgacgag ataatcggca cggagctcca tcgcgagcgg ctcaccgtcg acgagcggcg
     1681 cccatacgac gcgtgcctgc cgctcgacga tcgtgaaccc ggcgtcggcg agcagcagtg
     1741 ccgcggcatc ctcgccagcg cctgcacgtg ccgcccgtcg cttcgcacgc agcgagccgc
     1801 gccaactccg gatcacgctg gcgagccaca gcgcgaatag tgcggcaccg atcgcgacaa
     1861 cgattaactg cgtcacgcat cgatcgtcgc agcgcgatct gacatgttga gcttatatat
     1921 gagagtcgag ctcgtttacg gcgacatcac gacgctcgat ccggctgtag atgtgatcgt
     1981 caatgccgcg aacgagacgc tgctcggcgg cggtggcgtc gacggtgcga tccatcgcgc
     2041 cgccggcccc gagctgcgct gggagtgcgc gaagctcgac ggctgcgata ccggcgatgc
     2101 gaaggtcacg aaggcgtatc gcttgcccgc gaagcacatc atccacaccg tcggtccggt
     2161 gtgggaaggc ggcacgatcg gcgaagccgc actgctcgcg agctgctatc gcaagtcact
     2221 ccagctcgcg gccggcctcg gcgcgacgtc gatcgcattt cccgcgatct cgaccggcgt
     2281 ctacggctat ccgctgcgcg atgccgccga gatctcgatc aagacgatcc tcgcgcaccc
     2341 gttcgcgcag cccgagcgta tcgtgctgtg cacgttcgac cccgaaacca cggaggcgac
     2401 gcgcgagatg ctcgccgcgc tgactgccta ctaacttcta ctgatgcaga tcgagatcct
     2461 gcaaggcgac atcacgaagc tcgacgtcga cgcgatcgtc aacgcggcga actcgtcgtt
     2521 gctcggcggc ggcggcgtcg acggcgcgat ccatcgcgcg gcgggccccg acttgctcga
     2581 ggagtgcatc ctgatccgca atcgtgacgg gggctgcccg accggcgacg ccaagatcac
     2641 gaagggctat cgattgccgg cgaagcatgt gatccacacc gtcggtccgg tctggaatgg
     2701 cggcagccgc ggcgagcccg agctgctcgc gagctgctac cgcaagtcgt tgctactcgc
     2761 tgccgaggct ggcgcgaagt cgatcgcatt cccagcgatc tcgaccggca tctacggcta
     2821 tccctttcgc cccgccgccg agatcgcggt gcgcacgatc cgcgcgcaca ccggcgagct
     2881 tcccgagcgc gtcatcctgt gcacgttcga cgagcacgcg accgagatca cgcacgaggt
     2941 actcgccgct agcgactcag caagcgcgcc ggcgccgacc gagcaagccg acgaaggcga
     3001 agccgagtaa cgccgtcggt ccaccgcggc cactcgcatc gcagcagcca ccgccatcgc
     3061 caggatcgtc gggtgacagc acgcgcaccg catcgaacag gagcgtcgtg tcggtggccc
     3121 ccgcctcgcc ggtattatcg gcgagcatga tgtgctcgtc tccggtgcca gcgaagtcaa
     3181 actcgccgag cgagacccac ccactcgcgc tcgtctgatc gacaacgacc tcgtcggtcg
     3241 cgccggcgtg aacgatcacg tacttcgcct gcttcgacgt gccgaacgtg ccgccgtcca
     3301 ggttgacctc gacggagaat cgcgacgcgc ggctcgtctt gacgatccag agcgcgaagt
     3361 tcgcagcggc ggcgttgctc gtcgtgcccg tccactcgcg atcgtcgtcg tagccgccgg
     3421 tctcgcggtg ccagtagcgc ggatcgccgg cggcgagata gcactcgctg cgctgatcga
     3481 tcacgccgcc ctgcggaatc gcggcgcagc gatccgacgt acgccagaaa tcggcgccca
     3541 tctcgttgaa cgccatgatc gcgttgtcgc gatagcccgc agcgcgcgac tgacacgtcg
     3601 cactcgtcgt gcagcagccg ttgtagcgac acgcgagaaa gctcgcccac tcttccgtct
     3661 tcggatcgcc cgcctgcatc ggcacgccgt tcatccagcc aaccgcgccg agccagtcat
     3721 cgatgccagc gatgctcgtc tgcgagcgct cgacgacgaa gttcaccgcc tgcgccgtgt
     3781 tgccttcgat cgtcaggatg ctgtcgccat acgtcgcgac cgtctgcgcg tacgtgccgg
     3841 cgtcgaactg aaacataccg aggccgccct gcatgtccgc acacggtcca tccgccgcgc
     3901 ccgcgatgat cggcccgccg ttgcacgacg gcgacgccgg tccctgacac gcatacgtcg
     3961 cttcgctctg gcaatgggcg aggttcgtct cgctgatcgc gatacccgcc atcagcgccg
     4021 cgttgtagag gcccatctcg gccgccgagt cgcggatcag cccgtaccgc tcgtggcgca
     4081 ccgccgctgt gagctccgca cggtcctcgc cgagcgtcgg ttccgacgca cacgcggtaa
     4141 caagcagtaa accggcgacg agcctcacgc gacacccatg ctaaccacag cgcgcctcac
     4201 cctgccaggt ggtttgaggc gcacacctgc cggctcgcgt gtctactcag catggtgccg
     4261 ctgcggtttc tcctcgtgat cctcgtcgca ttgccgggtt gcccgtactg cgacttcgat
     4321 acaccaccac tcgacacaga tgtcacgcgc gagaaagcgc tcgacgaggc gttccatctc
     4381 gatccgcgct ggctcggcgg cgatgccgcc tacacgatcg atctcggcga cgacacctcg
     4441 ctgtggctgt tcggcgattc gttcatcgca acgtcgccga acttcacgcg cagcgagtcg
     4501 acgatggtgc gcaactcggt cgcgctgatg ggtggccgcg acctcgcaac cgcgtcgatg
     4561 caattcgcct ggcgcgacgg tgcaccgccg acgtcgttct tccccgaaga tgccgaccac
     4621 tggttctggc cgaacggcgg cgtgcgggtg ccgaacggcc cgctgctcgt gttcctctcc
     4681 gagctgcgcg cgacgccggg cgaaggtctc ggctttgccg gcgtcggtat gcgcgcactg
     4741 cgcgtcgcgg atccatccgg ctcgccgctc gactggacac tcgagccgac aaccgtgcac
     4801 tcgcccgcgt tcgcaccgac gatgaacgtc tcgtgctcga ccgtcgtcga cgacaagtat
     4861 ctcattgcgg tgttcaccgg cgacggcaat cacgacggga tgctcgcgcg ctggccactc
     4921 accgcgatcg cgaatggtga cgacctcggc aaccccgagt ggtggaccgg cacgatctgg
     4981 ctgcgggaga gctttctgac caagctgccg aaggtcgtga ttcccgacgg tggcagcgag
     5041 tgctcgttgc actacgacac catcggcagc gcaggttggg tgtacacgtg gagccgcggc
     5101 ttcggcgaca ccacgatcgc gatgcgcacg gcatgggagc tcaccggacc gtggtcggat
     5161 ccgttcgaca tcttcacgcc gccggagtcg aaggtggaca acgcgttcgt ctatgcggcg
     5221 aaagcgcatc cagcgctgat cggtccgaac ggcggattga tcattacgtt cgccgacaac
     5281 agcttcacgt tcgccgatct gttcgacgcc gcgaaggcgg ccacgctgta ctggccgcac
     5341 gtcgtcgagg tgctgatcta ccagatcgtc tgttagaagt cggtgccgat accggcctgc
     5401 agcacgagcg ggttgagcca catgctcatc ttcgcggtgc cgacctcgac gttgtcgaac
     5461 agcggtagat ccggcgtctt cacctgcacg tgctcgacgg tggcgcgcgc gagcatgaac
     5521 gcgatgaact tgacgtcgag gcgcgcgtag atccgcttcg tgatcatcgc gtcgatgccg
     5581 gcttgcagca cgaggcccgg cgccggcgcg atcgacatct gcggctcggc cactgcggtc
     5641 agctgcatgt tcgtcacctt ctcgttggtg gcgaacatga tcgacggacc tgcgccgaca
     5701 tacggcgtga ccttgccgag ctgcgcgacc tgatagacga gcgtcgcgac gatcggcacc
     5761 gctttcgccg tgccgagatc cgaaccgagc ggcggtacac cggtcggcaa cccgagcgcg
     5821 gtcggcgcga gcgaatcggt cgcgagcgtg ccggtcgcgt ggaacgtcac cgtgaacggc
     5881 aagccgagga tcgtctcgac cgacagcttg tcgttgccga ccggcaacac gtagccaacg
     5941 atgatgccgg gaatcgtcgc cgagctgacc gacgcgccag agcccgcgat cgggccgttc
     6001 atcagcgcga ggctcgcggg accgtcgacg tccgacagct ccatctcgcg cgactgttcg
     6061 agcggcgcga tatgcgcgac accggcgcga acgtagaagc gcttgcgctt cggcttggcc
     6121 ggcgcgacgg gcggctcggc agccggcgca tccgtcgtgg cagccgccgg cgcatccgtc
     6181 gtgggcgcga cctcgtcgtc ggcgtgcgcg agcgacggtg cgaggagcgt ggcgagtaac
     6241 agtagacgtt tcatagcgac actccgcgct ccgggggcga gaccagcttg agtttcatca
     6301 tgcctttggg cacgagcagg ttgatgctgc cgtcgccgat cagcggcggg ctgatgttga
     6361 cggtgattgc gaggtctgcg cggttcgcga gcgagatcgc gatgcgtccg tcaggcaaga
     6421 actcgagcgg tccctcgagg atcacactca gcttcttcga gtgcaggtcg tgggtcgcac
     6481 cgagcggcag gctcatgtcg ggcgcgtcca tgtagaggtc gagcgcgacg atcatcgtcg
     6541 gcgtaccgct acgatcgatg atgtagccca tcaccggccc ccccgcgggc tcgcggatgc
     6601 gcatcacctg gacgccggtc ggagtcgaga gaccgccgac cacgggaagg ttcgcagtca
     6661 tcgagatcga cgtgccgtac atcgcctgcg ccgacagcgt caccggcacg cacgacggcg
     6721 cgctctcccc gccgggcagc gggcacgtcg tcgtcacgtc gcccatcacc gccggcatcg
     6781 agccgacgag atacatgcag ttctccttct ccggcgttga cggtacacag tcgggatcgt
     6841 cgaacgacgc gctgttcacg ctgccgcccg tgccggtgat ccgcatcgct gcgctgttgt
     6901 cgtcgcgcag ctgctcgctg gtgtcgcgga caccggagcc attgacgtcg gtgaagggga
     6961 acgggctcgt catcaggaac gtgcccccgc tcttctctgc accgacgtac gtgaccgcga
     7021 gcgcaggacc gccaccgtcg ccgccgttgg caccggcgag cggatcgaag tttgcggcgg
     7081 tcgacgtgcc gcacagctca ccggcatcgc aggtgtcgtt gccgcccgag atgagcgtga
     7141 ggcggtagcg ctcgcccggc gtccacggtt gatcggggat aaacgacagc gagcgatcgt
     7201 gcgtcagcaa cgtgcctggc acgacgccgg tgcacgtacc tgccatatcg accttttcga
     7261 tgcgcacgtc gccttggttg cacgcggtgc cgcgcacgac ctgattgcgg cggatcggcg
     7321 ccgtgaactc gaccgcgatc ggctcgtccg cgccgagcgt gaacggcttg tagagatcat
     7381 cgctgctcgc accgccggcg catcggcccg ggctgttcgc ggtcgcaccg gtcagcgcgc
     7441 atgcgacgcc gggatgcacg gccgagaccg tcgggctgac gttcgtcgac aacagcgtcg
     7501 gcgtcacgaa gctgaggttg ttcatcgtca gcttgtttcc agcgacgtcc gcgacgtcgt
     7561 tgaacacgac gcgatagatc ttcgagtacg cgagcggcgc gagcggccgc acgacgacgg
     7621 ccgcaccatg gctctcgatc gtggtcgcga cgacaccggc cacggtgtcc
//
LOCUS       DASUFX010000664         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1773442, whole genome shotgun sequence.
ACCESSION   DASUFX010000664 DASUFX010000000
VERSION     DASUFX010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773442"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1136)
                     /locus_tag="VFV99_26910"
     CDS             complement(195..1136)
                     /locus_tag="VFV99_26910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063046.1"
                     /translation="MGNGLIGSTVLGRYQIEKELGKGSMGTVYRGIDLQRQREVAVKV
                     MRDDLVHERKLLDRFKREARVAAKMQHPNLMRVLDAGETRDGRPVMVLELARGTTLTT
                     LLEVSPAPRRVVRLVEQILLGLEHAHAAGLIHRDLKPDNVMVELGPDGTEILRIVDFG
                     IAILRMRDGGIEGGRLTETGQIVGTPLYMAPELAKEEPYDHRVDLFALGVIMYEMLAG
                     TVPFHGKTPVDIQLAYISKDPPPISTYVADVDPLHEAFTRKLMARKVAQRFASASEAL
                     RVLRLLETDPRYALLCMGIADTETASGVIGLRDPRRR"
     gene            1365..1562
                     /locus_tag="VFV99_26915"
     CDS             1365..1562
                     /locus_tag="VFV99_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX5063047.1"
                     /translation="MAEGTIKRLTDKGFGFIANDSGKDLFFHMSAVEGVRFEDLREGQ
                     RVSYNEGMGPKGPRAENVKPV"
     gene            1680..1925
                     /locus_tag="VFV99_26920"
     CDS             1680..1925
                     /locus_tag="VFV99_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063048.1"
                     /translation="MVQSQSAGSLLVRPGVPFNAVKVFSATMFADRDQLGEKVTRWVA
                     DHSHVQVTEMVVTQSSDASFHCIAITVFYRDPSRSQA"
     gene            2078..2773
                     /locus_tag="VFV99_26925"
     CDS             2078..2773
                     /locus_tag="VFV99_26925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063049.1"
                     /translation="MADELGMSQEALQQALAESRAGVLVPAEERTFVDQVFGTRIVEA
                     RRFVPGDINALRTAVDQFLEEQGFQLKRNLGHAQVWEAGRDFWTRLRRAFRSGAYRLP
                     RDVEIETRVTEVPGGPHPVLVALRVDASRARTARVGTATASVIAGAAGIAAGIALLPM
                     PTEILAIAGGGLAGVGGTWLSRSSFRDERERLTVALERFLDFLEHEPLSASQPASDPI
                     KRIVDFLSGDWWR"
     gene            2915..3736
                     /locus_tag="VFV99_26930"
     CDS             2915..3736
                     /locus_tag="VFV99_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063050.1"
                     /translation="MLILLAPLAACSHATSDIGGDLDAGADDGSDNGSADVDAPAPVG
                     PDLTCLSQPQPMPPTIAVDPMVLAGRVVEFTTSGVTPVDAADVVLFVAGQPTVLARTH
                     SASNGAFATGAVTTNGHAVHAYLKATKPNYRTAFFYPPLPFTANSTTLVVPTLSDAVF
                     ATAKTLLGATQDDRHNGALLVAVLDCDGQPLAGATLSVRRGNSSVGHAYDLGTVVPAD
                     AGVFIVFDVPDGKVNVSASYQGAQLPEHDVMVRGSDPDCSPPRGTLTSTIVQPNI"
     gene            complement(3742..>4351)
                     /locus_tag="VFV99_26935"
     CDS             complement(3742..>4351)
                     /locus_tag="VFV99_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063051.1"
                     /translation="YELGCLLHREIGDQRASFSPTIQIPVARGHKDDPLRLRRRIVSA
                     TTSVRFVDGVPEPLTAFEGRVRGVFAREAAATGLVSRLLGAARAVPMPVAWKRKSISA
                     KRPRWLETFADVIGGRALLSRIALDTSIPVMCAASSPARHASERDPIGGCVITLVDNG
                     EHAAITIAGSGFAGTPATASAFLDRLLDAAAQQPPIDHVQRR"
BASE COUNT          713 a         1451 c         1421 g          766 t
ORIGIN      
        1 cgaggcgccg atcgatgccg gcggcacggg cgacttcagc tacggcgtcg caaccggcga
       61 cttcaacagc gacggcaagc ccgacttcgc tacggcgaac gcgctctcca acgatgtcgc
      121 ggtcaagatg agcaccgcgc actgagcgct ggcggcattg ggctctcacc cgaaatactc
      181 ccgagatgag ggagtcagcg ccgtcgcggg tcgcgcaggc cgatgacgcc acttgccgtc
      241 tcggtgtcgg cgattcccat gcacagcagg gcgtagcgcg gatcggtctc gagcagccgc
      301 agcacgcgca gcgcctcgct tgccgacgcg aaccgctgcg cgacctttcg tgccatcagc
      361 ttgcgcgtga acgcctcgtg caatggatcg acatcggcga catacgtcga gatcggcggc
      421 ggatctttcg agatgtacgc gagctgaata tcgaccggcg tcttgccgtg gaacggcacc
      481 gtgccggcga gcatctcgta catgatcacg ccgagcgcga acagatcgac gcggtgatcg
      541 tagggctctt ccttcgcgag ctccggtgcc atgtacagcg gcgtcccgac gatctggccg
      601 gtctcggtga ggcggccacc ctcgatgccg ccgtcgcgca tgcgcaggat cgcgatgccg
      661 aagtcgacga tgcgcaggat ctcggttcca tcaggaccga gctcgaccat gacgttgtcg
      721 ggcttgaggt cccgatgtat cagtccggcg gcgtgcgcgt gctcgagacc gagcaagatt
      781 tgctcgacga ggcgcaccac gcgccgcgga gctggactga cctcgagtag cgtcgtcagc
      841 gtcgtgccgc gtgcgagctc gagcaccatc accggccggc cgtcgcgggt ctcgccggca
      901 tcgaggaccc gcatcaggtt cgggtgctgc atcttcgcgg cgacgcgcgc ttcgcgtttg
      961 aaccggtcga gcagcttgcg ttcgtggacg aggtcatcgc gcatgacctt gacggcgacc
     1021 tcgcgctggc gctggagatc gatgccgcga tacacggtgc ccatcgagcc cttaccgagc
     1081 tctttctcga tctgataccg accgaggacg gtgctcccga tcaggccatt ccccacgcta
     1141 gatcgagggt atcacgacga cgtttgcgct cgccggaggg caggcctcgg ggtgcgcggc
     1201 accggatcac tgggcgtttg tcggccatca gtcgccctgc tacccgcctg gctactgcgc
     1261 caagtccgcg cactggcgta agacttggcc cgcgttcgcg gttgccatgt cggttgtcgc
     1321 gctaccgcat cgacaatcgg ccgcaactcg tgacaagtag cggaatggcc gaaggtacga
     1381 tcaagcgtct gaccgacaag ggttttggat tcatcgcgaa tgactcgggg aaggatctgt
     1441 tcttccacat gtccgccgtc gagggcgtcc gcttcgaaga cctccgtgag ggacagaggg
     1501 tctcctacaa cgagggcatg ggccccaagg gcccgcgcgc cgagaacgtc aagccggtct
     1561 gaggactagc gccggcttgt cgaggggacg ccgttgattt tttagcaacg gcgcgcagct
     1621 aggcgtttgc ctgagctgac agggctgggt tccggataag acggtgctac gttcgcgcga
     1681 tggtgcagtc gcagtccgcg ggtagcctcc tcgttcgccc gggtgtcccg ttcaatgccg
     1741 tgaaagtgtt ctccgcgacg atgttcgcgg atcgcgatca gctcggcgag aaggtcaccc
     1801 gctgggtcgc cgatcactcg cacgtccagg ttaccgagat ggtcgtgacg caatcgagcg
     1861 acgcctcgtt tcattgcatc gcaatcacgg tcttctaccg cgatccgtca cgatcccagg
     1921 cgtagcgacg ccgcgtctgc cgggtagaat cggctgcgcc atgcccgagc agctgacgcg
     1981 caagcaggtc gagctgatcc tgcgccgtac cgcggagctc gagcagaagc gtgacgagac
     2041 cgcgctcgat gtcctcacgc cgcaagacct cgagcgcgtg gccgacgagc tcggcatgtc
     2101 gcaggaagcg ctccagcagg cgcttgcgga atcccgcgcg ggcgtgctcg ttccggccga
     2161 ggaacgtacg ttcgtcgatc aggttttcgg cacccgtatc gtcgaggcgc gccggttcgt
     2221 cccgggcgac atcaatgccc tgcgcaccgc ggtcgaccag ttcctggaag agcaaggctt
     2281 ccagctcaag cgcaacctcg gccatgccca ggtctgggag gcaggtcgcg atttctggac
     2341 gcggctgcgt cgcgccttcc gctccggcgc gtaccgcctg ccgcgcgatg tcgagattga
     2401 aacgcgcgtc accgaagtac caggtggccc gcacccggtc ctggttgcgc ttcgggtcga
     2461 tgccagccgc gcacgcaccg cgcgggtagg taccgccaca gcctcggtta tcgcgggtgc
     2521 cgcgggcatc gctgccggca tcgcgttgct gccaatgccg acggagatcc tcgcgatcgc
     2581 gggcggcggc ctcgctggtg tcggcgggac gtggctctcg cgttcgagct ttcgcgacga
     2641 gcgtgaacgg ttgacggtcg cgctcgaacg cttcctcgac ttcctcgagc acgaaccact
     2701 ctcagcgagt cagccggcaa gcgatccgat caaacggatc gtcgactttc tctccggcga
     2761 ctggtggcgc tgatctccgt tcgcgttacg aacgcgactc gatcgctctt cgacgcaact
     2821 cgtcgacgga agtcggtcag aactagccag taatccgata tggattgctt tcgtcgcgcg
     2881 cgttagtcga cgcgcgatga tgaaacagct ctccttgctt atcttgctcg cgccgctcgc
     2941 ggcgtgctcc cacgccacca gcgatatagg tggcgatctt gacgctggcg ccgacgatgg
     3001 cagcgacaac ggcagcgccg atgtcgatgc gcccgcgccg gtcggccctg atctcacctg
     3061 cctctcgcag ccgcagccga tgccgccgac gattgccgtc gatccgatgg tgctcgctgg
     3121 ccgtgtcgtc gagttcacga cctcaggcgt gacacccgtc gacgcagcgg acgtcgtgtt
     3181 gtttgtcgcc ggtcaaccga ccgtgctcgc tcgcacgcac agcgcttcga acggcgcgtt
     3241 cgcgaccggt gccgtcacca ccaacggtca cgcggttcac gcgtacctca aggcgacgaa
     3301 gccgaactac cgcacggcgt tcttctatcc gccgctgccg ttcacggcaa actcgaccac
     3361 gctcgtcgtg ccgacgctct ccgacgcagt attcgcgacg gccaagacgc tgctcggcgc
     3421 gacgcaggat gatcggcaca acggcgcgct cctcgtcgcc gtgctcgact gcgatggtca
     3481 accgctcgcc ggcgcgacgc tcagcgttcg ccgcgggaat tcgtcggtgg gccacgcgta
     3541 cgatctcggc actgtcgttc cggcggacgc cggcgtgttc atcgtgttcg acgtgcccga
     3601 cggcaaggtc aacgtgtccg cgagctatca aggcgcgcaa ctgccggaac acgacgtgat
     3661 ggtccgcgga tcggaccctg actgctcgcc tccgcgcggc acgttgacgt cgaccatcgt
     3721 ccagccgaac atttgaggcg attagcggcg ctgcacgtga tcgataggag gttgctgtgc
     3781 ggcagcgtcc agcagtcgat cgaggaacgc agacgcagtc gccggcgtgc ccgcgaatcc
     3841 tgatcctgcg atcgtgatcg cggcgtgctc gccgttgtcg acgagcgtga tcacgcaacc
     3901 accgatcgga tcgcgctcgc tcgcgtggcg cgctggcgac gatgcggcgc acatcacggg
     3961 gatcgacgtg tcgagcgcga tgcgagacaa cagcgcgcga ccgccgatca cgtcggcgaa
     4021 cgtttcgagc cagcgcgggc gcttggcgct gatgctcttg cgcttccacg cgacaggcat
     4081 cggcaccgcg cgcgcggctc cgagcaatcg cgacacgagg cccgtcgcgg ccgcttcacg
     4141 tgcgaacaca ccgcgcacgc gtccttcgaa tgcggtcaac ggttccggca cgccatcgac
     4201 gaagcgcacg ctcgttgtcg ccgagacgat gcggcgacgc agccgcaacg gatcgtcctt
     4261 gtggccgcgt gcgacgggaa tctggatcgt tggcgagaag ctcgcgcgct ggtcgccgat
     4321 ctcgcgatgc agcaagcagc cgagctcgta c
//
LOCUS       DASUFX010000665        10462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_940388,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000665 DASUFX010000000
VERSION     DASUFX010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940388"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFV99_26940"
     CDS             complement(<1..717)
                     /locus_tag="VFV99_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063052.1"
                     /translation="MNCPAPARLAAAATGEDLGVLDHARGCLACRAALIEQGEVIRAA
                     RMLKHPSLAADRRAALAAEVMARADVADAQRPWRGERVMAIAASVVAAAAVVTIVLAS
                     GGPRPQIPAAPQIAADEEPAVHTSRPTHLDVVAAPLRANVVGTGAELSRALHGEQDVV
                     TLREGEVSVDATDREPVTIVAGDTRVVIASSRAKVVARRGVIVTAQVFAGTAQVTSGG
                     RQQVIDAGDVWMHAPEPKPAV"
     gene            complement(714..1289)
                     /locus_tag="VFV99_26945"
     CDS             complement(714..1289)
                     /locus_tag="VFV99_26945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5063053.1"
                     /translation="MKKGQLIQLRPQVRVDEMSDVGLLAACAAADRTARALLFERHVD
                     SVHRFVSRMRASDADIVDDLVQATFMRAFQSAARFHGTSARSWLYGIAANVVREHARK
                     EIRRKRAMSVVAEELPRTAQARDPHLLARLPDAIAALPHELREAFVIVDLEGEKGCDA
                     AAALGIPEGTLWRRLFHARTALRKAIDGGRS"
     gene            complement(1286..2209)
                     /locus_tag="VFV99_26950"
     CDS             complement(1286..2209)
                     /locus_tag="VFV99_26950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063054.1"
                     /translation="MTRLVALAIVLAAAPAAADPCDVRIVHAPDAVRAEVAKWLLDEA
                     TCGGPLEIRIVPTTEGLYVFARDINGRVRERVVPDAQSAGVLIASWAASDADAPKPAM
                     SVELSYSPPKAALAPPRDALVRRYTPARASQQRTLAMQTFFGSTIVGMRGDYAVWRKG
                     PWLASATMSIAHDDYIDWENRGHDKLDFIDTKLMASVAAIGETDRVRITWGVSAGVVA
                     SYVRGTSYFDDDPMQPSYAYGKLDVLGVSPALEATATIGVRLNREWMVEGGALATVFT
                     QEYETPTTMGHTTIEARGAKLLFLAGVRRSL"
     gene            complement(2206..3186)
                     /locus_tag="VFV99_26955"
     CDS             complement(2206..3186)
                     /locus_tag="VFV99_26955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063055.1"
                     /translation="MKHALVLAIVLAASSASAQPAARGSCEVTFVRVPETVRPIVERW
                     VQAEPKCNTKLEIRIVPTAGGLYLLARDPSGRIRERVVPDAQSAGVLVASWIAADSLS
                     PYEVRKPPTPEVAPVPPEAMLGSDGQLLAPGEGAAPGAAGPVITHAVAAEKAPGKPRW
                     LSLGPLIGLTGPGGGLRGEWDAKMYGSWVFGAAASTSRVGMAYLDGYGYDTIDMFDTK
                     ALAYAGLVGEGGHWHMRGTAGVGVVYTRAHLDMTASGPQSAEGVFPTAELQLMVGRDF
                     GDHWAIDIGPILSIYAQEMQLESSGAGYYSYMTANRDVAMTMFGALRYRL"
     gene            complement(3183..4094)
                     /locus_tag="VFV99_26960"
     CDS             complement(3183..4094)
                     /locus_tag="VFV99_26960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063056.1"
                     /translation="MTRAIVFASLLAATAAGAQPITRPTACGVTIAYASADVRIVVDA
                     FVAQETTCNATLEVRIVPTDGGLYLFARDERGRVRERVVPDAQAAAVLVASWMADDTR
                     PPVPPPIAVFDHHVPPATVTVDPFDRVPSAEHESHGWLFGLGPTLAGAGAGVRAEIDV
                     ASWGAWSIGVAASLVDNEATLYITQQADQSMDVIDSTGLVYVTRRTDLGDWYLRPALG
                     VGFTYTQMTTRMDYSYDPFQPWPPGIEYQADGTVATVDAQLLLGRRLGDHWQLDAGPG
                     VTVFADGPGLLQRSPQRSLFAALRYRL"
     gene            complement(4091..5044)
                     /locus_tag="VFV99_26965"
     CDS             complement(4091..5044)
                     /locus_tag="VFV99_26965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063057.1"
                     /translation="MRSVIYAVVLLVASSATAQPVTEPKACQVTIARAPDDVRAVVET
                     WVQSEPQCSVALEVRIVPTEGGLYLLAQDEHGRIRERIVPDAQTAGVLVASWIADDNA
                     PPVVLPPPAPVIPVAQPAAAIETNESVTPPGLAPVSLTATASRPVRRSKWLTAAVMLP
                     IGEGAGQGVRADIDVFRRGRWYAGLAASLGAGEMPLMSSSGTGFIHTKDYKGAIYMAR
                     PSQFGRWELRPTVGVGVMHTQGLAYDGSSFYSLDGTFTTLEASVLLSRELGNSWAIYG
                     GPLATVINQRYEVSVSYQDFPVSLSRGNIDLVMLTGVRHRL"
     gene            5266..6843
                     /locus_tag="VFV99_26970"
     CDS             5266..6843
                     /locus_tag="VFV99_26970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063058.1"
                     /translation="MIRAPWSTGSSEEEARAYLQARLTVLYKLMFWCFVILIGFLWVT
                     YWRFHDIKPDLQIWVNTAFVTGMAIEGFIWRGLLVRKPRMSFEALNALDTFFVVAANT
                     VIAGCALLSYDQRQAAYTCLIYSCFAVLTRALVLPSTGRRTAIVSALGAIPMAIGAII
                     LPFIKDWPPGKDVPGPGFVLGFLQIEVVTILLAAAGSEIIYGLRQKVSAAQQLGQYTL
                     GRKIGEGGMGVVYLAHHDLLRRPTAVKLLHPDRVGHDSMQRFEREVRHMSQLTHPNTV
                     AVFDYGRSFDGAFYYAMEYLAGIDLHNLVVKHGKQPAERVRQIMIQVCGALQEAHDRG
                     IVHRDIKPANIILCERGAMPDVAKVVDFGLVKEFTANTGASTQVVVGTPAFVAPEAVT
                     DPNAVGPAADLYALGCVGYFLLTGRKVFDAKTAVDLCIQHVTKQPVPPSQIGSTHVPR
                     QLEEILMTCLAKQPSGRPASANDLADRLRAAGSFDDWDDARASTWWRDFKPPQDLDTT
                     AIPTTTITVDVGRRKVA"
     gene            6840..8456
                     /locus_tag="VFV99_26975"
     CDS             6840..8456
                     /locus_tag="VFV99_26975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063059.1"
                     /translation="MNAGTSGRVSRQMVRAMWSGDASEEEARAFLQTRIAFLFKLMFW
                     SMVALLAFLAALYAIDEANAPARRYEVYLISSIGLGGMAFIWRVWLIQPKPLSSTMMY
                     RLDLVYSLGIGTAFGAAAYLQNDLRASGYLSVVYSTLTVFARALIVPSSGKRTAVASS
                     ITMLPMTAAALALAIERAQDVPRDVFFIGYMLFCIVPIVMSSYGSQIIYGLRQKEAAA
                     KQLGAYTLDRKIGEGGMGVVHLAHHVMLRRPTAIKQLLPDRVGPDNLARFEREVQHMS
                     QLTHPNTVAIYDYGRSHDGIFYYAMEYLGGCVDLQRLVTKHGPQPSGRVAQILAQVCG
                     ALHEAHTNQIIHRDIKPANIILCERGGMPDVAKVVDYGLVKEFAAETGSSTQVVLGTP
                     AYLSPEAVTDPRSIGPGADIYALGAVGYFLLTGQKVFDGKTAVEVCIAHVTKAPRPPS
                     EVAKQPIAAPLEAIILKCLAKKPEDRYASVIELRSALLAVPAIDWSVAQARQWWGEFR
                     AVEQSVSDASDGATLTLDLEHHRGAFPVSS"
     gene            8564..8737
                     /locus_tag="VFV99_26980"
     CDS             8564..8737
                     /locus_tag="VFV99_26980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063060.1"
                     /translation="METVVLVVLAVVVALVAIDAIARLDERKQPRLPPSSSGRDETMI
                     STQKIIVDLTKRR"
     gene            complement(8709..9572)
                     /locus_tag="VFV99_26985"
     CDS             complement(8709..9572)
                     /locus_tag="VFV99_26985"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5063061.1"
                     /translation="MGCLCCGTGAEVGSLCRTCAQEVSPCEGLIPDHIRSNVEATEAV
                     GWVVDGFGAAHPLSAKTGIGRSHEGELVILASSVSREHAELRRTDAGWHIRDLGSRNG
                     TFVDGTRAQGRATLGERTLVKIGDVALWFLAEAEHDPIAPPSMATASAGGGLVRFNIT
                     PGAVELCLVGTTDLGAGGALLSRSNGGDGWTERSLAPLEFQLLRALCTRAVEEADSPS
                     AVRGCVQTKQLARELPFQSKYANEENVRQVVRRLRQELGGIGCESVLAVAPGRGYYLS
                     CPVSVASSGQR"
     gene            complement(9624..>10462)
                     /locus_tag="VFV99_26990"
     CDS             complement(9624..>10462)
                     /locus_tag="VFV99_26990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063062.1"
                     /translation="IAKAVELTSPLLEQRNHQLTLDIPRELGIEADATRMTQVFANLM
                     TNAAKYTQPRGHISITALRVNETIAVTVSDDGPGIAPELLPNIFDAFVQGQSTLERAE
                     GGLGLGLALVRSLTELHGGTASAASDGHGRGARFTVQLPAYQVVTTPAMPQPSVAPGA
                     RLLLVDDNADAARVLAELLREHGYDVAVAHDAPAALLLADEFRPVIALLDIGLPVMDG
                     YELARHLRTRMPTPLRLVAVTGYGDHADTARSREAGFDVHLGKPVDFDKLVSVLGSLS
                     PS"
BASE COUNT         1741 a         3613 c         3367 g         1741 t
ORIGIN      
        1 gaccgccggc ttcggttcgg gtgcatgcat ccacacgtcg cccgcatcga tcacttgctg
       61 gcggccgccg ctcgtgacct gcgcggtgcc ggcgaatacc tgtgcggtca cgatgacgcc
      121 gcggcgcgcg acgaccttcg cacgcgagct cgcgatcacg acccgcgtat cgccagcgac
      181 gatcgtcacc ggctcgcgat cggtcgcatc gaccgacact tcgccttcgc gcagtgtgac
      241 gacgtcctgt tcgccgtgca gcgcgcgcga tagctcggca cccgtgccga cgacgttcgc
      301 acgcaacggc gcagccacca catcgagatg cgtcgggcgc gatgtatgga cggctggttc
      361 ctcgtcggcc gcgatctgcg gcgccgctgg gatctgcgga cgcggtccgc ccgatgcgag
      421 cacgatcgtc acgacggctg ccgccgcgac gacgctcgca gcgatcgcca tcacgcgctc
      481 accgcgccac ggccgctgcg catcggccac atctgctcgc gccatcacct cggccgcgag
      541 cgccgcacgc cgatccgcag cgagcgatgg gtgcttcagc atccgcgcgg ccctgatgac
      601 ctcaccttgc tcgatcagcg ccgcccggca tgcgaggcag ccgcgtgcat gatcgagcac
      661 accgagatct tcgccggtgg cggcggcggc gagccgtgcg ggcgcggggc aattcacgag
      721 cgacctccgt cgatcgcttt acgcagtgcc gtgcgcgcgt ggaacagccg gcgccacagc
      781 gtgccctcag gaatgccgag tgccgccgca gcatcacagc ccttctcgcc ctcgagatcg
      841 acgatgacga acgcttcgcg cagctcgtgc ggcaatgctg cgatcgcgtc gggcagccgc
      901 gccagcaagt gcggatcgcg cgcttgtgcc gtgcgtggca gctcctcggc cacgacgctc
      961 atcgcgcgct tgcgccggat ctccttgcgc gcgtgttcgc ggacgacatt cgcggcgatg
     1021 ccgtacagcc atgaccgcgc gctcgtgcca tgaaaccgtg ccgcgctctg aaacgcgcgc
     1081 atgaacgttg cctgcacgag atcgtcgacg atatcggcgt cgctcgcgcg catgcgcgac
     1141 acgaaacgat ggaccgagtc gacgtggcgc tcgaacagaa gcgcccgcgc cgtgcgatca
     1201 gcggcggcgc acgcagcgag gaggcccacg tcggacatct cgtcgacgcg cacctgcggg
     1261 cgcagctgga tcagctgccc cttcttcaca gcgaacgcct cacgccggca aggaacagca
     1321 gtttggcacc acgcgcttcg atcgtggtgt gccccatggt cgtcggcgtc tcgtactcct
     1381 gcgtgaacac cgtcgcgagc gcgccgccct cgaccatcca ctctcgattg aggcgcacac
     1441 cgatcgtggc agtcgcttcc agcgcgggcg agacgccgag cacgtcgagc ttgccgtagg
     1501 catacgacgg ctgcatcgga tcgtcgtcga agtagctcgt gccccgcacg tacgacgcga
     1561 caacaccagc gctcacgccc caggtgatgc gcacgcggtc ggtctcgccg atcgcggcaa
     1621 ccgacgccat cagcttcgta tcgatgaaat cgagcttgtc gtggccgcgg ttctcccagt
     1681 cgatgtagtc gtcgtgcgcg atcgacatcg tcgccgacgc cagccacggt cccttgcgcc
     1741 acacggcgta gtcgccgcgc ataccgacga tcgtcgagcc gaagaacgtc tgcatcgcga
     1801 gcgtccgctg ctggctcgcc cgcgccggcg tgtaccggcg aacgagcgca tcacgtggtg
     1861 gtgcgagcgc agctttcggc ggcgaatagc tgagctcgac actcattgcg ggtttcggcg
     1921 catcggcatc cgatgccgcc cagctcgcga tcaacacgcc tgcgctctgc gcatccggaa
     1981 ccacgcgttc gcgcacgcga ccgttgatgt cgcgcgcgaa cacatagagg ccttccgtgg
     2041 tcggcacgat gcggatctcg agcggcccgc cgcacgtcgc ctcgtcgagg agccacttcg
     2101 cgacttccgc gcgcaccgca tccggcgcgt gcacgatccg gacgtcgcac ggatctgccg
     2161 cagccggtgc ggccgctagc acgatcgcca gtgcgacgag tctcgtcaca gccgatacct
     2221 caacgcaccg aacatcgtca tcgcgacgtc gcggttcgcc gtcatgtacg agtagtagcc
     2281 agcgccgctg ctctcgagct gcatctcctg cgcgtagatc gacaggatcg gtccaatgtc
     2341 gatcgcccag tggtccccga aatcgcgacc gaccatcagt tgcagctcgg cggtcgggaa
     2401 cacgccctcc gcgctctgcg gaccgctcgc cgtcatgtcg agatgcgctc tcgtgtagac
     2461 gacgcccacc ccggcagtgc cgcgcatgtg ccagtgcccg ccttcaccga cgagcccggc
     2521 gtacgcgagc gccttggtat cgaacatgtc gatcgtgtcg tagccgtagc cgtccaggta
     2581 cgccatgccg acgcgcgacg tcgaggccgc ggcgccgaac acccacgaac cgtacatctt
     2641 ggcgtcccac tcaccgcgca acccgccacc gggtcccgtc aatccgatga gcggacccag
     2701 cgagagccag cgcggcttcc cgggcgcttt ctcggccgcc accgcatgcg tgatgaccgg
     2761 acccgccgca ccgggcgccg cgccttctcc gggcgcgagc agctggccat cactgccgag
     2821 catcgcctcc ggcgggaccg gcgcgacctc gggcgtcggc ggcttgcgca cttcgtacgg
     2881 cgagagcgaa tccgctgcga tccagctcgc gacgagcacg cctgcgctct gcgcatcggg
     2941 cacgacgcgt tcgcggatgc ggcctgacgg atcgcgcgcg agcagataca acccacccgc
     3001 agttggcacg atgcggatct cgagcttcgt gttgcacttc ggctccgctt gcacccagcg
     3061 ctcgacgatc gggcgcacgg tctccggaac gcggacgaac gtcacctcgc agctaccgcg
     3121 tgccgcgggt tgtgcggaag ccgacgatgc ggccagcacg atcgcgagga caagcgcgtg
     3181 tttcatagcc ggtacctcag tgccgcgaac agcgagcgtt gcggcgagcg ttgcaacaga
     3241 cctgggccat ccgcgaatac cgtcacgccc ggcccggcat cgagctgcca gtggtcgccg
     3301 agtcggcggc cgagcagcag ttgtgcgtcg acggtcgcga ccgtgccgtc cgcttgatac
     3361 tcgatccccg gtggccacgg ttggaacgga tcgtaggagt agtccatccg cgtcgtcatc
     3421 tgcgtgtacg tgaagccgac gccgagcgcg ggtcgcaggt accagtcacc gaggtccgtc
     3481 ctgcgcgtga catagacgag accggtcgag tcgatgacgt ccatcgactg gtccgcttgc
     3541 tgagtaatgt agagcgtcgc ctcgttgtcg acgagcgatg cagcgacacc gatgctccac
     3601 gcgccccagc tcgcaacgtc gatctcggcg cgcacaccgg cgccggcacc tgcgagtgtc
     3661 gggccgaggc cgaacagcca accgtgtgac tcgtgctccg cgctcggcac gcgatcgaac
     3721 ggatcgaccg tcaccgttgc cggcggcaca tggtgatcga agacggcgat cggtggcggc
     3781 accggtggcc tagtgtcatc cgccatccag ctcgcgacca gaaccgcagc cgcctgggcg
     3841 tcggggacga cgcgctcgcg cacccgaccg cgctcgtcac gcgcgaacaa gtacaacccg
     3901 ccgtcggtcg gcacgatgcg aacctcgagc gtcgcgttgc acgtggtttc ctgcgcgacg
     3961 aacgcatcga cgacgattcg cacgtcagcg gacgcatacg cgatcgtcac gccgcaggcg
     4021 gtcggccgcg tgatcggctg ggcacccgcc gccgtcgctg cgagcagcga agcgaaaacg
     4081 atcgcgcgcg tcatagtcgg tgacgaactc cagtgagcat tacgaggtcg atgttgccgc
     4141 gcgacagcga gaccggaaaa tcctggtagc tgacgctgac ctcgtaccgc tggttgatca
     4201 ccgtcgcgag cggaccaccg tagatcgccc agctgttgcc gagctcgcga ctcagcagca
     4261 ccgacgcttc gagtgtcgtg aacgttccgt cgagcgagta gaacgacgag ccgtcgtacg
     4321 cgagcccctg cgtgtgcatg acgccaacgc ctactgtcgg ccgcagctcc cagcgcccga
     4381 actgcgaggg ccgcgccatg tagatcgcgc ccttgtagtc cttcgtgtgg atgaagcccg
     4441 tgccagatga cgacatcagc ggcatctcgc ctgcgccgag tgacgcagcc aggccggcgt
     4501 accaacgtcc gcgccgaaac acgtcgatgt cggcgcgcac accttggcct gcaccttcgc
     4561 cgatcggcag catcactgcg gcggtcagcc atttgctgcg gcgaacgggc cgcgaagcgg
     4621 tcgccgtgag cgacaccggc gcgagaccgg gcggggtcac cgattcattc gtctcgatcg
     4681 ccgcggccgg ctgcgccacc ggaatgacgg gcgccggtgg cgggaggacg accggcggcg
     4741 cgttgtcgtc ggcgatccag ctcgcgacca gcacgcccgc ggtctgggca tcgggcacga
     4801 tgcgttcgcg gatccggccg tgctcgtctt gcgcaagcaa gtacaggcca ccttcggtcg
     4861 gcacgatgcg cacttcgagt gcgacgctgc actgcggttc cgattggacc cacgtctcga
     4921 ccaccgcgcg cacgtcatcg ggcgcgcgcg cgatcgtgac ctgacacgct ttgggctcgg
     4981 tcaccggttg tgctgtcgcc gacgacgcga ccagcagaac gactgcataa atgacgctgc
     5041 gcatgaccct ccctgcggcc gtcggtatca cgactgttgg tggccgcaca caccagtttt
     5101 catctcggtc ttggcgcaat tttgcagacg ttttttgctc ccggcgctgg cagctgctgg
     5161 ccggatctcc cttgttaccc gcgtgaaatc cacgtgatcg ttcgccacct ctggcagaat
     5221 cgcaggatcg tggtgaacgt ggcggaacca ggccgctctc gccgaatgat ccgcgccccc
     5281 tggagcactg gctccagcga ggaggaggcg cgcgcctacc tccaggcccg gctgacggtc
     5341 ctctacaagc tgatgttctg gtgcttcgtc atcctcatcg gcttcctgtg ggtgacgtac
     5401 tggcgcttcc acgacatcaa gcccgacctg cagatctggg tgaacacggc gttcgtcacc
     5461 ggcatggcga tcgagggctt catctggcgc ggcctgctcg tgcgcaagcc gcggatgtcg
     5521 ttcgaagcgc tcaacgcgct cgacacgttc ttcgtggtcg ccgcaaatac cgtgatcgcc
     5581 ggctgcgcgc tcctgtcgta cgaccagcgg caagccgcat acacgtgcct gatctactcg
     5641 tgcttcgccg tgctcacgcg cgcgttggtc ctaccgagca ccggccggcg caccgcgatt
     5701 gtcagcgcgc tgggcgcgat cccgatggcg atcggcgcca tcatcctgcc gttcatcaag
     5761 gactggccgc cgggcaagga cgttcccggc cccgggttcg tgctcggctt tctgcagatc
     5821 gaggtcgtga cgatcctgct tgccgctgcc ggctccgaga tcatctacgg cctgcgacag
     5881 aaggtcagtg ccgcgcagca gctcggccag tacacgctcg gccgcaagat cggcgaaggc
     5941 ggcatgggcg tcgtctacct cgcgcaccac gacctattgc gccgaccgac cgccgtgaag
     6001 ctcttgcacc ccgatcgcgt cggccacgac agcatgcagc ggttcgagcg cgaagtgcgg
     6061 cacatgagtc agctcacaca tccgaacacc gtcgccgtgt tcgattacgg ccgcagcttc
     6121 gacggcgcgt tctattatgc aatggagtat ctcgctggca tcgatctcca caacctcgtc
     6181 gtcaaacacg gcaagcaacc ggctgaacgc gttcggcaga tcatgatcca ggtgtgcggt
     6241 gcgctacagg aagcgcacga ccgcggcatt gtccatcgtg acatcaagcc cgcgaacatc
     6301 atcttgtgcg agcgcggcgc gatgcccgac gtcgcgaagg tcgtcgattt tggcctcgtc
     6361 aaagagttca cggccaacac cggcgcgtcg acgcaggttg tcgtcgggac cccggcattc
     6421 gtcgcacccg aggccgtgac cgatccgaac gccgtcggcc cggcagcgga tctctatgcg
     6481 ctcggctgcg tcggctactt cttgctcacc ggccgcaagg tgttcgatgc gaagaccgcc
     6541 gtcgatctgt gtatccagca cgtgacgaag cagccggtgc cgccgtcgca gatcggcagc
     6601 acgcacgtgc cgcgtcagct cgaagagatc ctgatgacgt gcctcgcgaa acagccgagt
     6661 ggacgaccgg cgagcgcgaa cgatcttgcc gaccgcctgc gtgctgctgg ctcgttcgat
     6721 gattgggacg acgcgcgcgc cagcacgtgg tggcgcgact tcaagcctcc acaggacctc
     6781 gataccaccg cgatcccgac gacgacgatc accgtcgacg tcggtcgccg aaaggtcgca
     6841 tgaacgcggg cacctcggga cgcgtctcgc ggcagatggt ccgcgcgatg tggagcggcg
     6901 atgcgagcga ggaagaagcg cgcgcattcc tccagacgcg catcgcgttt ctgttcaagc
     6961 tcatgttctg gagcatggtc gcgctgctcg cgttcctcgc ggcgctctac gcgatcgacg
     7021 aggcaaacgc gcccgcacgc cgctacgaag tgtatctgat ctcgtcgatc ggcctcggcg
     7081 ggatggcgtt catctggcgc gtgtggctga tccagcccaa gccgctatcg agcacgatga
     7141 tgtaccggct cgacctcgtg tactcgctcg gcatcggcac tgccttcggt gcggccgcgt
     7201 atctccagaa cgacctgcgt gcgtcggggt acctgtccgt cgtctacagc acgctcacgg
     7261 tgttcgcgcg cgcgctgatc gtgccgagca gcggcaagcg tacggcagtg gcgagctcga
     7321 tcacgatgct gccgatgacc gccgctgcac tcgcgctcgc gatcgagcgc gcgcaggatg
     7381 tgccccgtga tgtgttcttc atcggctaca tgctgttctg catcgtgccg atcgtgatgt
     7441 cgtcgtacgg ctcgcagatc atctatggcc tgcgtcagaa agaggcagcc gcgaaacagc
     7501 tcggcgcgta cacgctggat cgcaagatcg gcgaaggcgg catgggtgtc gtgcatctcg
     7561 cgcatcacgt gatgctgcgc cgcccgaccg cgatcaagca gctgcttccc gatcgcgtcg
     7621 gcccggacaa cctcgcgcgc ttcgagcgcg aggtgcagca catgagtcag ctcacacacc
     7681 cgaacacggt cgcgatctac gactacggcc gcagccacga cggcatcttc tactacgcga
     7741 tggagtacct cggcggctgc gtcgatctgc agcgactcgt caccaagcac ggtccccagc
     7801 cgagcggccg cgtcgcgcag atcctcgcgc aggtctgcgg cgctttgcac gaggcgcaca
     7861 cgaaccagat cattcatcgc gacatcaagc cggcgaacat catcctgtgc gaacgcggcg
     7921 gtatgcccga cgttgcgaag gtcgtcgact acggcctcgt gaaggagttc gcagccgaga
     7981 ccggttcgtc gacgcaggtc gtgcttggca cgccggccta cctgtcccct gaagcggtca
     8041 ccgatccgag gtcgatcggt ccgggcgccg acatctacgc gctcggcgcc gtgggctact
     8101 tcctgctgac cggccagaag gtgttcgacg gcaagaccgc ggtcgaggtc tgcatcgcgc
     8161 acgtgacaaa ggcgcccagg ccgccatccg aggtcgcgaa gcaaccgatt gctgcgcccc
     8221 tcgaggcgat catcctcaag tgtctcgcca agaagcccga ggaccgctac gcgagcgtga
     8281 tcgagctacg cagcgcgttg ctcgcggtgc cagcgattga ctggagcgtc gcgcaggcgc
     8341 gccagtggtg gggtgagttt cgtgcggtcg agcagtcggt cagcgacgcg tctgacggcg
     8401 ccaccctcac attggatctg gaacatcatc gcggcgcctt cccggtttcc tcgtgaaaag
     8461 cgcgtgcatc cggacggtgg atctcggaca acgcgagtga tttcgcacgt gagaatcgcg
     8521 tgaataggtc ggaccactgg ccttgatgag ctacaccgta ggcatggaaa cggtggtcct
     8581 cgtggttcta gcggtcgtgg tcgcgctggt cgcgatcgac gccatcgcgc gactcgacga
     8641 gcgcaaacag ccgcgcctgc cgccgtcatc atctggccgc gacgagacca tgatctcgac
     8701 gcagaagatt atcgttgacc tgacgaagcg acgctgaccg gacacgacag gtagtaaccg
     8761 cgtcccggcg cgaccgcgag cacgctctcg cagccaatgc cgccgagctc ctgacggagc
     8821 cgtcgcacga cttggcggac gttctcttcg ttcgcgtact tcgactggaa cggcagctca
     8881 cgcgcgagtt gcttcgtctg cacgcaaccg cgaactgccg acggcgagtc cgcttcttcg
     8941 accgcgcgtg tgcacagcgc gcggagcagc tggaactcga gcggtgcgag gctacgctcg
     9001 gtccagccgt cccctccgtt gctacgcgag agcagcgcgc cgccggcgcc caggtcggtc
     9061 gttccgacga ggcagagctc gacggcaccc ggtgtgatgt tgaagcgaac cagaccaccg
     9121 ccagcgcttg cggtcgccat cgatggtggc gcgatcgggt cgtgctctgc ttcggcgagg
     9181 aaccacaatg cgacgtcgcc gatcttcacg agcgtgcgct ccccgagcgt ggccctgccc
     9241 tgcgcgcgcg tgccgtcgac gaacgttccg ttacggctgc cgagatcgcg aatgtgccag
     9301 cccgcatcgg tgcggcgcag ctcggcatgc tcgcgcgata ccgagctcgc aaggatcacg
     9361 agctcgcctt catggctgcg tccaatgccg gtcttggcag atagcgggtg cgcagcaccg
     9421 aagccgtcga caacccagcc gactgcttcg gtcgcctcga cgttcgagcg gatgtggtcc
     9481 gggatcaggc cctcgcacgg cgacacttct tgcgcgcacg ttcgacagag cgagccgact
     9541 tcggccccag taccacaaca cagacagccc atggtcagga ccatcgaacc acctcttgcg
     9601 tccaggcaca aggccctgac cggttagctc ggcgatagcg atcccagaac cgatacgagc
     9661 ttgtcgaagt cgaccggctt gccgaggtgc acgtcgaagc cagcttcacg gctacgcgca
     9721 gtatccgcgt ggtcgccgta gccggtcacg gcaaccaacc gaagtggtgt cggcatgcgg
     9781 gtgcgcaaat ggcgcgcaag ctcgtagccg tccatgacgg ggaggccgat gtcgagcagt
     9841 gcaatgacag ggcggaattc gtcggcgagg aggagcgcag ccggtgcgtc gtgcgcgacg
     9901 gcgacatcgt agccatgttc gcgcaacaac tcggccaaca cgcgagccgc gtccgcgttg
     9961 tcgtcgacca gcaaaagccg cgcgcccggc gcaaccgatg gctgcggcat ggcgggggtg
    10021 gtgacgacct gataggccgg aagctgcacg gtgaaccgcg caccgcggcc gtgtccatcg
    10081 ctcgctgcac tggctgtgcc gccgtgcagt tcggtgaggc tccgtacgag tgcgaggcca
    10141 aggccaagcc caccctccgc gcgctcgagc gtgctctgac cttgcacgaa cgcgtcgaag
    10201 atgttcggca gcagctcggg cgcgatgccc gggccgtcgt cgctgaccgt gacggcgatc
    10261 gtctcgttca cgcgcagcgc cgtgatcgag atgtggccgc gtggctgcgt gtacttcgcc
    10321 gcgttcgtca tcagattcgc gaagacctgc gtcatgcgtg tcgcgtctgc ttcgatcccg
    10381 agctcgcgcg ggatgtcgag cgtcagctga tgattgcgct gctcgagcag cgggctcgtc
    10441 agctcgaccg cttttgcgat ca
//
LOCUS       DASUFX010000666         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_661754,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000666 DASUFX010000000
VERSION     DASUFX010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661754"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFV99_26995"
     CDS             complement(<1..454)
                     /locus_tag="VFV99_26995"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5063063.1"
                     /translation="MAKGVAARIDANERLGNCNIENISVGGVFIRTQTGMPIGMPVRV
                     DLTKRGVEPLQLSGRVVSVVSETEAKRQDISPGVGIEFDPMPLATEKQLHALLRELGL
                     KDLADPIEPRDLYATTSPDTAQVTSNVQGLVAMLTDALQKVKDRDDEIA"
     gene            complement(500..1006)
                     /locus_tag="VFV99_27000"
     CDS             complement(500..1006)
                     /locus_tag="VFV99_27000"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5063064.1"
                     /translation="MQPQTEAHKKPQTLRVESSAFGANGSIPAQFTADGEDIAPPLTW
                     TTPPAGTKSIAIIVDDPDAPNPAAPQRTWVHWVVTGIPATTTSLESDKSLPAGAAVGT
                     NDFGKRAWGGPNPPIGRHRYFFKLYALDIDLATPGITKLELLAAMKGHILAQGELIGT
                     YAKPHARH"
     gene            1069..>2553
                     /locus_tag="VFV99_27005"
     CDS             1069..>2553
                     /locus_tag="VFV99_27005"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5063065.1"
                     /translation="MKIACIGGGPAGLYLGILVKRSAPEHDVVVYERNRPADTFGFGV
                     VFSDATLGHLAAADPETHAQITAQFARWDDIEIHVGGEVLRSTGHGFCGLERKTLLQI
                     LQARAKQLGVRIEFEHEIKSLSEVSADVIVACDGVASWVRDALAAELQPKVEVRPNKF
                     VWLGCTVPYQAFTFIFKQTPFGLFRVHAYRYQQTGSTFIVECREDTWRAAGLDTADED
                     RTLAILGDVFAAELGGHKLIKNRSIWRNFPTVRLGKWHAGNVVLMGDAVHTAHFSIGS
                     GTKLAMEDAIVLRDQLLATRDVPAALAAYEAKRRPEVEALQAAAQASLEWFEGTERYM
                     NMAPVQFTYSLMTRSLRVSHASVAKRDPHLARGVEQLLAGKVGITPGKAGITPALGDH
                     RETPPDQRLPPPTALPCALGDRRATNRIALSPIAAPAPRAADNKAVPIAEGLAGDDEL
                     VALGAAAAMGAGLVVSPPILVTVAVAQRDADGSLTRGGDTPRINT"
BASE COUNT          414 a          865 c          823 g          451 t
ORIGIN      
        1 tcgctatctc gtcgtcgcga tccttcacct tctgcaacgc gtcggtgagc atcgcgacca
       61 ggccctgcac gttcgatgtc acctgcgcgg tatctggact ggtcgtcgca tagagatcgc
      121 gcggctcgat tggatcagcg agatccttga ggccgagctc gcgcaacagc gcgtgcagct
      181 gcttctcggt cgcgagcggc atcgggtcga attcgatgcc aacgcccggc gagatgtcct
      241 ggcgcttggc ctcggtttca ctcacgacgc tgaccacgcg gccactcagc tgcagcggtt
      301 cgacgccacg cttcgtgagg tcgacgcgca ccggcatacc gatcggcatc ccggtctggg
      361 tacgaatgaa cacgccgccg accgagatgt tctcgatgtt gcagttgccg agtcgttcgt
      421 tggcgtcgat ccgcgccgcg acacccttcg ccacgactct gcgatgccgg cgatgtttca
      481 cacctccaca ttaacaagat cagtggcgcg cgtgcggctt cgcgtaggtg ccgatcagct
      541 caccctgcgc gagaatgtgc cctttcatcg cagcgagtag ctcgagcttc gtgatgccgg
      601 gcgtggcgag atcgatgtcg agtgcataga gcttgaagaa gtagcgatgg cgaccgatcg
      661 gcgggtttgg tccgccccag gcgcgcttgc caaagtcgtt cgtgcccacg gcggcacccg
      721 cgggaagtga tttgtcgctc tccagcgacg tcgtggtggc cgggatgccc gtcacgaccc
      781 agtgcaccca cgtacgttgc ggcgccgccg gatttggcgc atccggatcg tcgacgatga
      841 tcgcgatgct cttggtgccg gcaggcggcg tggtccacgt cagcggcggc gcaatatcct
      901 cgccgtcggc ggtgaactgc gcgggaatcg agccattcgc cccgaacgcc gacgactcga
      961 cgcgcagcgt ctgcggcttc ttgtgagctt cggtttgtgg ctgcatagcg aaccctactc
     1021 cccacctctc gacgcggcga tgtcgaattc ttctgatagt gttcgcacgt gaagatcgcc
     1081 tgcatcggcg gcggcccggc cgggctctat ctcggaattc tcgtcaagcg cagcgcgccc
     1141 gagcacgacg ttgtcgtgta cgagcgcaac cggccggcag acacgttcgg attcggcgtc
     1201 gtgttcagcg atgccacgct cggccacctc gcagccgcgg atccagaaac ccacgctcag
     1261 atcacggcgc agttcgcgcg ctgggacgat atcgagatcc acgtcggtgg cgaagtgctc
     1321 cgctcgaccg gccacggctt ctgcggactc gagcgcaaga cgctgctcca gatcctgcag
     1381 gcgcgagcga agcagctcgg tgtgcgcatc gagttcgagc acgagatcaa gtcgctttct
     1441 gaagtgagcg ctgacgtcat cgtcgcgtgc gatggcgtcg cgagctgggt gcgcgatgcg
     1501 cttgccgcgg agctacagcc gaaggtcgag gtccggccga acaagtttgt ctggctcggc
     1561 tgcaccgttc cttatcaggc gttcacgttc atcttcaaac agacgccgtt cgggctgttt
     1621 cgcgtccacg cctatcgcta tcagcagacc ggctcgacat tcatcgtcga gtgccgcgag
     1681 gacacgtggc gcgctgctgg cctcgacaca gccgacgaag atcgcacgct cgcgatcctc
     1741 ggcgatgtgt ttgccgccga gctcggcggt cacaagctga tcaagaatcg ctcgatctgg
     1801 cgcaacttcc cgaccgtgcg cctgggcaag tggcacgccg gcaacgtcgt gctgatgggc
     1861 gatgccgttc acaccgcgca cttctcgatc ggctcgggca cgaagctcgc gatggaagac
     1921 gcgatcgtgc tgcgcgatca gctgctcgcc acgcgcgacg tgccggcagc gcttgccgcc
     1981 tacgaagcga agcgccgccc ggaggtcgaa gcgctacaag ctgcagctca ggcgagcctc
     2041 gagtggttcg aaggcaccga gcgctacatg aacatggcgc cggtccagtt cacgtactcg
     2101 ctgatgacgc gcagcctgcg cgtctctcac gcgtcggtcg ccaagcgcga tccgcacctc
     2161 gcgcgcggcg tcgagcagct gctcgcgggc aaggtcggca tcacgcccgg caaggcgggc
     2221 atcacgcctg ccctcggcga tcaccgcgag acgccgcccg accagcgcct gccgccgccg
     2281 actgcactgc cgtgcgcgct cggcgatcgc cgcgcaacga atcgcatcgc gctctcgccg
     2341 attgcggccc ccgccccgcg cgcggcggac aacaaggcag tgccgatcgc cgaaggtctc
     2401 gccggcgacg acgagctggt cgcgctcggt gcagccgctg cgatgggcgc cgggctcgtc
     2461 gtcagcccgc caattctggt gaccgtcgcc gtcgcgcagc gcgacgccga cggatcattg
     2521 acacggggcg gcgacacgcc gcgcatcaac acc
//
LOCUS       DASUFX010000667         8231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_105868,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000667 DASUFX010000000
VERSION     DASUFX010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105868"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFV99_27010"
     CDS             <1..265
                     /locus_tag="VFV99_27010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063066.1"
                     /translation="PKMCVATYRLGRVYFAREEWEKAAESFQTVSEDPSCGSQEASYY
                     LMKTRMQQGLVSEAKSARTSCLKISPNSCIATKCRADGGGLGP"
     gene            330..1667
                     /locus_tag="VFV99_27015"
     CDS             330..1667
                     /locus_tag="VFV99_27015"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5063067.1"
                     /translation="MSLATWLRAGRDRRKLTIDDVAKITKIQPRILEKLESGQLEGLP
                     AEVFVKGFVRSFAKCVGLDETEALDKYSAAQAAPNRRDNSDSGSFAIAKALVETMQRP
                     IDVETVIEAKRVAQGTPVEPAPVVTAPIEAAPVEAAPIEAAPIEAAVIEPAPVSVAAP
                     AIEASVAPVIEAQVVAAAVASEQPQPTKKKRSRKNSKQRAKHKKQVQEELAAVAAAVA
                     ADVAAVVDNDAVETSVFEKQPIDTGPVASIFASGDGESLEASSIEAKTTEPLSTASEP
                     EPTDGVVESAPLAEGSNPTITIPEHASGLGLDIPAAAALDATSGETTQATWQPTMPPI
                     ATPSMPWRRPNLPTQTASRAPVVPSLLIDDADPDSADREREDRIAKEPNRLSFLPPIL
                     LDREDRSARQGGLTLAVIILLIAATLTLSYLMRRPSPSGDGVTMLDGASQLIS"
     gene            1678..2463
                     /gene="recO"
                     /locus_tag="VFV99_27020"
     CDS             1678..2463
                     /gene="recO"
                     /locus_tag="VFV99_27020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5063068.1"
                     /translation="MAEGELGVVLKTTPLRESDLLVTLYTETHGRVAAVARGARKSQR
                     RFAGALQLLVLGRYQLGRRPRGELWALDAAEVEREWTQLASDVFAVAHASYVAELVGA
                     LLPPEQPEPHVLDVVVALWTSLAEGGPSPAALRAVELELLDVAGHRPALEACAACGEV
                     DLATGAVFDPTRGGAICKRCAATSRGANIRPIDAATLGYLQAVAAAGSPSAARVLDAD
                     PRVDPRDRAAGRDAMIAMVQSLVGKPLKSLEYIAKLGAAGRRP"
     gene            2476..3585
                     /locus_tag="VFV99_27025"
     CDS             2476..3585
                     /locus_tag="VFV99_27025"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5063069.1"
                     /translation="MGNRRLALIVGALLCSSSSTFAQPAEGEIEMEGDPEPAPTSPDQ
                     NAQPPSDQPPPIVKDPKVAKKWQQAGDQLMKKGDYLTGKGKPDAKTMYENAVTAYQHA
                     IEASDDASLNYQLALAEDKAGMTPDALKHLKVVLAAQGIKPAITKNAQAKLDELSMKV
                     GVVTLTIAPDGTQVSIEGKQVGEAPMTEPLVLMPGVHKVSLTAVGFQPKDVELKIEAG
                     SESERKIALEPVPVVNKQQVEEPEPIPVAAPKGPDKRPLYIGGGIGAGFILVATVTGI
                     VAITKHGTYTDDNATPAERADAQSSGKTFAHVTDFCIVGAVAAGAFTAYWYQYKYRPQ
                     ARALSERQQPQTEAKVDVVPWVQPEAGGLSVAGRF"
     gene            3597..4439
                     /locus_tag="VFV99_27030"
     CDS             3597..4439
                     /locus_tag="VFV99_27030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063070.1"
                     /translation="MATGYPPGMPPMGGPPPMGGPPGMGGPPGMGGPPGMGGPPGMGG
                     PPGIGGPPMGPPGMPPMGGPQMGMHRPMRRGTPKIVPIIMSAGLAVGVFCGLLFGVGK
                     KSEAVASTPSKGTNVKPPAEEPKPDPGGAPAGLGATSTKPVPPAQHTAPAAGSAAVAA
                     APAGAGSAAPAAKPAPTVTEEKKNKLTVTLKPEAAMTGAKLFIDGKEVEGMTVDIPVD
                     KKSVKVEVKSPGYRSVEKKIDITGETMSVEFETVKRPASTPGVKPPKRPDRPPSGGGG
                     LIDI"
     gene            complement(4436..4729)
                     /locus_tag="VFV99_27035"
     CDS             complement(4436..4729)
                     /locus_tag="VFV99_27035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063071.1"
                     /translation="MPTLLITIKPAAAAEVATIQIDGTPLLGTRLELESPARVHVSVT
                     AIGFTRFAKDILVDKDMTLEVDLTKRAHKRNRTLAVSLGMGAIGVLAWLVRRR"
     gene            complement(4744..5595)
                     /locus_tag="VFV99_27040"
     CDS             complement(4744..5595)
                     /locus_tag="VFV99_27040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5063072.1"
                     /translation="MNRRPRLLTIIAVHIALIALVAVVLYPVMLVCKKAFEPGRNFAL
                     SASPIPQDVTVDHFKDLFGARGGHGELLFLRHALNSLIVALSTTVVGVVLSCTAAYAL
                     SRFRFPGRKAGLTTFLVVQMFPATLLLLPLYVILDKLGLLNSLVGLVLVYSTTAIPFC
                     VWTLKGYFDTLPRELEEAARIDGASTWMIFRRIILPLARPGIAVTALFSFMTAWNEFI
                     MASTFMTDESKYTLPVLIQSSVGQFSADWGLFAAGAVVTSIPVMVAFYVLQKYLVGGL
                     TAGAVKG"
     gene            complement(5592..7211)
                     /locus_tag="VFV99_27045"
     CDS             complement(5592..7211)
                     /locus_tag="VFV99_27045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5063073.1"
                     /translation="MIGESSYTRAPVLQRQLRDFAVVGALALLVATVAFVAVRNTELA
                     ARQTDIAQRAALELSEGKAPLPTVHGALVTPSTPSKKHPYLRHRQIREPDSAWRHLGG
                     MSTDPTHADDKLFYDAATRFEQLHQSELTEMMRDGSGRALAVRSVCTGKQCVLAIAVT
                     SPASPARYPWLVIIGVLGLGAAFAAAGALIGKGAARAGMFAGIGVLAVPTTLWHAWIA
                     TTAILLLAGGVALAQRAQLTDRLATGLARHRGALTFLTPAALAMLVLVAAPFVIGVLI
                     GFYDHHAGTWTFVGLDNFAEILSGGGRPLDDALNFWFILGVTVLWTVCNITLHVTIGV
                     SLALVMSRKWIFGRGIFRMLLILPWAIPNYITALMWKGMFDGQYGAINSLLHAVGLDG
                     VDWFTRWAPSFAANVATNTWLGFPFMMVVALGALETIPRELYEAASVDGASAWQRFRH
                     ITLPHLRPALGPAVALGSIWTFNMFNVIYLVSAGKPGNATNILVTDAYRWAFERGERY
                     GMAAAYGTVIFLILLLWTVFGTRIVRRKGDE"
     gene            complement(7208..>8231)
                     /locus_tag="VFV99_27050"
     CDS             complement(7208..>8231)
                     /locus_tag="VFV99_27050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5063074.1"
                     /translation="PLTLVAVPHDSFADKLTSAIPRGNGPDLFIFADDRIGAWAESHT
                     IEPIEFWVDDARADRFSPAAMKSLTYDGSLWGLPLATKSLVLYVRTDLVSTPPTTTDE
                     LIALAPMMRKQQGFAVAYANADLYGHAPWLHGFGGKVIDDSGELAIATPEAAAAMAFA
                     RKLVAEGVAPADAQAPMVASLFNAGKAATVLSGPWFIADIGKDVPWQVVTLPVISETG
                     KPAAPFLGAESVLMSAYAHDKTAAFAVMDALTSDDAAITRARVARQVVANPAAYTDAE
                     IARDPVLPVFRAQLEHTVPMPKVPAMRMVWTPYQNALGEVLAGRSDPSTQLENVRRDV
                     EGYLRR"
BASE COUNT         1532 a         2612 c         2883 g         1204 t
ORIGIN      
        1 gccgaagatg tgcgttgcaa cttatcgcct cggcagggtt tacttcgccc gcgaggagtg
       61 ggaaaaagcc gcagaatctt ttcagacggt gtccgaagac ccgtcctgcg ggtcccagga
      121 agccagttac tacctgatga agactcgaat gcagcaaggg ttggtcagcg aagcaaagag
      181 cgcgcgtacg tcgtgtctca agatctcgcc gaacagctgc atcgcaacga agtgccgcgc
      241 cgacggcggt ggcctggggc cttaagccaa gatcagagac agagatggat caacgtactc
      301 gagctgaggg tggaagcgtc gacgcacgga tgagcctcgc gacgtggctt cgcgcaggac
      361 gtgatcgtcg gaagctcacg atcgacgacg tcgcgaagat caccaagatc cagccgcgca
      421 ttctcgaaaa gctcgagagc ggccagctcg aaggcctgcc ggccgaagtg tttgtcaaag
      481 gcttcgtgcg cagcttcgcg aagtgcgtcg gtctcgacga gaccgaagcg ctcgacaagt
      541 acagcgctgc gcaagccgcg ccgaatcgcc gtgacaactc ggactcggga tcgttcgcga
      601 tcgcgaaggc gctcgtcgag acgatgcagc gtccgatcga cgtcgagacc gtgatcgaag
      661 cgaagcgcgt cgcgcagggc acgccggtcg agccggcgcc cgtggtcacc gcgccgatcg
      721 aagctgcgcc ggttgaagcc gcgccgatcg aagccgcgcc gatcgaagcc gcggtgatcg
      781 agcctgcgcc ggtgagcgtg gctgcgcccg cgatcgaagc ttcggtcgcg ccggtcatcg
      841 aggcgcaggt cgtcgcagcc gcggtcgcaa gcgagcagcc gcagccgacg aagaagaagc
      901 gttcgcgcaa gaacagcaag cagcgcgcga agcacaagaa gcaggtccag gaagagctcg
      961 cggcagtcgc tgcggcggtc gcggcagatg ttgccgccgt cgtcgacaac gacgccgtcg
     1021 agaccagcgt gttcgagaag cagccgatcg acacgggccc ggtcgcgtcg atctttgcct
     1081 ctggcgatgg cgagtcgctc gaggcgagct cgatcgaagc gaagacgacc gagccgctgt
     1141 cgacagcgag cgagccagag ccgaccgacg gtgtcgtcga gagcgcgccg cttgcggagg
     1201 gcagcaaccc gacgatcacg atcccggaac acgcgagcgg gctcggtctc gatatcccgg
     1261 cggcggcggc gctcgacgcg acgagcggcg agacgacgca ggcgacctgg cagccgacga
     1321 tgccgccgat cgcgacgccg agcatgccgt ggcgccgtcc gaaccttccg acgcagacgg
     1381 cgtctcgcgc gcccgtcgtg ccgtcgctgt tgatcgatga tgccgatccc gacagcgcgg
     1441 atcgcgagcg cgaggaccgg atcgcgaagg agccgaaccg gctctcgttc ttgccgccga
     1501 tcctgctcga tcgcgaggat cgctcggcgc gccaaggcgg cctgacgctc gcggtgatca
     1561 tcctgctcat cgcagcgacg ctgacgctca gctacctgat gcggcggccg agcccgagcg
     1621 gcgatggcgt gacgatgctc gatggcgcgt cgcagctgat cagctagata ttcggcgatg
     1681 gctgaaggcg agctcggcgt cgtcctcaag acgacaccgc tgcgagaatc cgatctgctc
     1741 gtcacgctct atacggagac gcacggtcgc gttgccgcgg tcgcgcgcgg cgcacgcaag
     1801 tcgcagcggc ggtttgccgg cgcgctgcag ctgctcgtgc tcggtcgtta ccagctcggt
     1861 cgccggccac gcggtgagct gtgggcactc gatgctgccg aggtcgagcg cgagtggacg
     1921 cagctcgcca gcgacgtgtt cgcggtcgcg catgcgagct atgtcgccga gctggtcggc
     1981 gcgctgctac cgcccgagca gccagagccg cacgtgctcg acgtggtcgt cgcgctgtgg
     2041 acgagcctcg cggaaggtgg accgtcgccg gcggcgttgc gagcggtcga gctcgagcta
     2101 ctcgatgttg cgggccaccg gccggcgctc gaggcgtgcg cggcgtgtgg cgaggtcgac
     2161 ctcgcgaccg gcgcggtgtt cgacccgaca cgcggcggtg cgatctgtaa gcggtgtgct
     2221 gcgacgagcc gcggggcgaa tatccgcccg atcgacgcgg ctacgcttgg ctacctacag
     2281 gcggtggcgg cggcagggtc gccgtcggcg gcgcgggttc tcgacgccga tcctcgcgtc
     2341 gatcctcgcg atcgggcggc cggccgcgac gcgatgatcg cgatggtcca gtcgctcgtc
     2401 ggcaaaccac tcaagtcgct cgagtacatc gcgaagctcg gcgccgccgg ccgtcgaccg
     2461 tagtatcttc gccgcatggg taaccggcgg ctcgcgctga tcgtgggcgc attgctttgc
     2521 tccagcagtt cgacgttcgc acagcccgca gagggcgaga tcgagatgga gggcgatccg
     2581 gaaccggcgc caacatctcc cgatcaaaac gcgcagccac cgagcgatca gccgccaccg
     2641 atcgtcaaag atccgaaggt cgcgaagaag tggcagcaag ccggcgacca gctgatgaag
     2701 aagggcgact acctgaccgg caagggcaag cccgatgcga agacaatgta cgagaacgcg
     2761 gtcaccgcgt atcaacacgc gatcgaagcg agcgacgacg cgagcctgaa ctatcagctc
     2821 gcactcgctg aagacaaagc gggcatgacg cccgatgcgc tcaagcatct gaaggtcgtg
     2881 ttagcggcgc agggcatcaa gccggcgatc acgaagaacg cgcaggcaaa gctcgacgag
     2941 ctctcgatga aggtcggcgt cgtcacgctg acgatcgcgc cggatggcac gcaggtgtcg
     3001 atcgagggca agcaggtcgg tgaagcgccg atgaccgagc cactcgtgct gatgcccggc
     3061 gtgcacaagg tgtcgctcac cgccgttggc tttcaaccga aggacgtcga gctgaagatc
     3121 gaggcgggct ccgagagcga gcgcaagatc gcgctcgagc cggttccggt cgtcaacaag
     3181 cagcaggtgg aagagcccga gccgattccg gttgcggcgc cgaagggccc ggacaagcgt
     3241 ccgctctaca tcggcggcgg catcggcgca ggcttcatcc ttgtcgcaac cgtcaccggc
     3301 atcgtcgcga tcacgaagca cggcacgtac accgacgaca acgcgacgcc ggccgaacgc
     3361 gcagacgcgc agtcgagcgg caagacgttc gcccatgtca ccgacttctg catcgtcggc
     3421 gccgtcgcgg cgggtgcgtt caccgcctac tggtaccagt acaagtatcg gccgcaagct
     3481 cgcgcgctta gcgagcggca acagccgcaa acggaagcca aggtcgacgt ggttccttgg
     3541 gtccaacccg aagccggcgg cctctcggtc gcaggtcgtt tctaagcgag gtcagcatgg
     3601 ctaccggata tccgccagga atgccgccga tgggaggccc gccgccgatg ggaggtccgc
     3661 ccggaatggg cggcccaccg ggaatgggtg gtccgccggg aatgggtggc ccgccgggaa
     3721 tgggtggccc gccgggaatc ggtggcccgc cgatgggtcc gccaggaatg ccgccgatgg
     3781 gtggcccgca gatgggcatg caccgaccga tgcgtcgcgg tacgccgaag atcgtcccga
     3841 tcatcatgtc ggcaggactc gcggtcggcg tgttctgcgg cctcttgttc ggcgtcggca
     3901 agaagagcga agcggtcgca tcgacgccgt cgaagggcac caacgtgaag ccacctgccg
     3961 aggagccgaa gccggatccg ggcggtgcac cagcgggtct cggcgcgaca tcgacgaagc
     4021 cggttccgcc ggcgcagcac acggcgcctg cagcgggttc cgcggcagtt gccgcagcgc
     4081 cagcaggtgc aggctcggcg gcaccggctg cgaagccagc gccgacggtt accgaagaaa
     4141 agaagaacaa gctgacggtc acgctcaagc cggaagcggc gatgacgggc gcgaagctct
     4201 tcatcgatgg caaggaagtc gaaggcatga ccgtcgacat tccggtcgac aagaagtcgg
     4261 tgaaggtcga ggtcaagtcg ccgggctatc gcagcgtcga gaagaagatc gacatcaccg
     4321 gcgagacgat gagcgtcgag ttcgagaccg tgaagcgccc cgcatcgacg cctggcgtga
     4381 agccgccgaa gcgtccggat cgcccgccga gtggtggtgg cggtctgatc gatatctagc
     4441 ggcggcgcac gagccacgcg agcacgccga tcgcgcccat gccgagtgac acggcgaggg
     4501 tgcgattgcg tttgtgcgcg cgcttcgtca gatcgacctc gagcgtcatg tccttgtcga
     4561 cgaggatgtc cttcgcgaag cgcgtgaaac cgatcgcggt gaccgagacg tgaacgcgcg
     4621 cagggctctc gagctcgagg cgtgtgccga gcagcggagt gccgtcgatc tggatcgtcg
     4681 cgacctcagc tgcagcggca ggcttgatcg tgatcaagag cgtcggcacg cccttagtat
     4741 acgtcagcct ttgacggcgc ctgcagtcag gccgccgacc aggtatttct gcaacacata
     4801 gaacgcgacc atcaccggga tgctggtgac gacggcaccg gccgcaaaca gcccccagtc
     4861 ggccgagaac tgaccgacgc tcgattggat caggaccggg agcgtgtact tcgactcgtc
     4921 ggtcatgaac gtcgacgcca tgatgaactc gttccacgcg gtcatgaacg agaacagcgc
     4981 ggtgaccgcg atgccggggc gggcgagcgg caggatgatg cggcggaaga tcatccacgt
     5041 cgatgcgcca tcgatacgtg cggcttcttc gagctcgcgc ggcagcgtgt cgaagtagcc
     5101 cttgagcgtc cacacgcaga atgggatcgc cgtcgtcgag tagacgagca cgaggccgac
     5161 gagcgagttg agcaggccga gcttgtcgag gatgacgtag agcggcagca gcagcagcgt
     5221 cgccgggaac atctggacga cgaggaacgt ggtgagaccc gctttgcggc cggggaagcg
     5281 gaagcgcgac agcgcgtatg cggcggtgca cgacagcacg acgccgacga cggtggtcga
     5341 caacgcgacg atcagcgagt tgagcgcgtg gcggaggaac aagagctcgc cgtggccacc
     5401 gcgcgcgccg aacagatcct tgaagtgatc gacggtgacg tcttgcggga tcggcgatgc
     5461 agagagcgcg aagttgcggc cgggctcgaa tgccttcttg cacacgagca tcaccggata
     5521 cagcacgacg gcgacgagcg cgatcagcgc gatgtggacc gcgatgatcg tgagcaggcg
     5581 agggcggcga ttcattcgtc ccccttgcgt cgcacgatgc gcgtgccgaa cacggtccac
     5641 aacaagagga tgagaaagat gaccgtgccg taggcggcag ccatgccgta gcgctcgccg
     5701 cgctcgaacg cccagcggta ggcgtcggtg acgaggatgt tcgtcgcgtt ccccggctta
     5761 cctgcagaca ccaggtagat gacgttgaac atgttgaacg tccagatcga gccgagcgcg
     5821 acggcgggcc cgagtgcggg acgtaggtgc ggcaacgtga tgtggcggaa gcgctgccag
     5881 gcgctcgcac cgtcgacgga tgcagcttcg tagagctcgc gcggaattgt ctcgagtgcg
     5941 ccgagtgcga cgaccatcat gaacgggaag ccgagccacg tgttcgtcgc gacgttcgcc
     6001 gcgaacgacg gcgcccagcg cgtgaaccaa tcgacgccgt cgagaccgac cgcgtgcagc
     6061 agcgagttga tcgcgccgta ctggccgtcg aacatgccct tccacatcag cgcggtgatg
     6121 tagttcggga tcgcccacgg caggatcagc agcatccgga agatgccgcg gccgaagatc
     6181 cacttgcgcg acatcacgag cgcgaggctc acgccaatcg tgacgtgtag cgtgatgttg
     6241 caaacggtcc acaggaccgt cacgccgagg atgaaccaga agttgagcgc gtcgtcgagc
     6301 gggcgaccac ctcccgacag gatctcggcg aagttgtcga gaccgacaaa cgtccatgtg
     6361 ccggcgtggt gatcgtagaa gccgatgagc acgccgatca cgaacggcgc ggcgaccagc
     6421 acgagcatcg cgagcgcggc aggcgtgagg aatgtcagcg cgccgcgatg gcgagcgagg
     6481 cccgtggcca gccgatcggt gagctgcgcg cgttgtgcga gcgcgacgcc gccggcgagc
     6541 agcaggatcg cggtcgtcgc gatccacgca tgccacagcg tcgtcggaac ggcgagcacg
     6601 ccgatgccgg cgaacatgcc cgcgcgcgcg gcgcccttgc cgatcaacgc acccgcggcg
     6661 gcgaacgcag caccgaggcc gagcacgccg atgatgacaa gccatggata gcgcgccggg
     6721 cttgccggcg acgtcaccgc gattgcgagc acgcattgct tgccggtgca caccgaacgc
     6781 accgcgagtg cgcgaccact gccatcgcgc atcatctcgg tcagctcgct ctggtgcagc
     6841 tgttcgaagc gcgtcgcggc gtcgtagaac agcttgtcgt cggcgtgcgt ggggtccgtc
     6901 gacatgccgc cgaggtggcg ccacgcgctg tcgggctcgc ggatttgccg gtgacgcagg
     6961 tagggatgct tcttggacgg cgtggacggc gtcacgaggg cgccatgcac cgtcggcagc
     7021 ggcgccttgc cttcgctcag ctcgagagcg gcgcgctgtg cgatgtcggt ctgccgtgcg
     7081 gcgagctcgg tgttgcgcac cgcaacaaac gcgaccgtgg cgactagcaa cgcgagcgcg
     7141 ccgaccaccg caaagtcgcg aagctggcgc tgtagcaccg gcgcgcgcgt gtatgacgac
     7201 tccccgatca tcgcctcaga tagccttcga catcgcggcg aacgttttcg agctgcgtac
     7261 tcggatcact gcgaccggcg agtacttcac cgagcgcgtt ctggtacggc gtccacacca
     7321 tgcgcatcgc cggcaccttc ggcatcggca ccgtgtgctc gagctgggcg cgaaacaccg
     7381 gcagcaccgg atcgcgcgcg atctcggcgt cggtatacgc agcgggattc gcgacgacct
     7441 ggcgagcgac gcgagcgcgc gtgatcgcag cgtcgtcgct ggtgagcgca tccatcacgg
     7501 caaacgccgc ggtcttgtcg tgcgcgtacg cactcatcag gacgctttcg gcgccgagga
     7561 atggtgcagc cggtttgccg gtttccgaga tcacgggcag cgtgacgact tgccacggca
     7621 cgtccttgcc gatgtcggca atgaaccacg gaccggacag cacggtcgct gctttgcccg
     7681 cgttgaacag gctcgcgacc atcggtgcct gcgcatcggc aggtgcgacg ccctcggcga
     7741 cgagcttgcg cgcgaacgcc atcgccgccg cggcctccgg cgtcgcgatc gcgagctcgc
     7801 cgctatcgtc gatcaccttg ccgccgaagc cgtggagcca cggcgcgtga ccgtagagat
     7861 cggcattcgc gtacgcgacg gcgaagcctt gctgcttgcg catcatcggc gcgagcgcga
     7921 tcagctcgtc ggtcgtggtc gggggcgtcg agaccagatc ggtacgcacg tagagcacga
     7981 gcgacttcgt cgcgagcggc aggccccaca gcgagccgtc gtaggtgagc gacttcatcg
     8041 cggcggggct gaaccgatcg gcgcgcgcgt catcgaccca gaactcgatc ggctcgatcg
     8101 tgtgcgactc cgcccacgca ccgatgcggt cgtcggcgaa gatgaacaga tcgggaccgt
     8161 tgccgcgcgg gatcgcgctc gtcagcttgt cggcgaacga gtcgtgcggc accgcgacga
     8221 gcgtcagtgg c
//
LOCUS       DASUFX010000668         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1495473, whole genome shotgun sequence.
ACCESSION   DASUFX010000668 DASUFX010000000
VERSION     DASUFX010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495473"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VFV99_27055"
     CDS             complement(<1..1650)
                     /locus_tag="VFV99_27055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5063075.1"
                     /translation="MQWLANVCVRRPIFASVLIAVFVVVGIVGYKSLGVDKFPKVDFP
                     MVMIVTPYPGASPGAVETDVSQKIEEAVNTVNGLDTLTSVSTEGVSLVLAQFQLEIDP
                     KDAADDVSEHIATVLRDLPPGVRPEVRRADPDAAPVIVLSVKGPKGMSTRDLTRFADK
                     SVKQRIERLKGVGQIVILGGQDRQINVNLDSIRLAATGVSALEVQRAIAGANVNVPGG
                     QFEMGPKNSTLRIEGRAIEAKQIGDIVVRQQGDHAVRVRDVADLEDAEEEAETAALRN
                     GVPAIALSVRKQTGTNTVAVVDAVTNAVQELKKQLPPDYSVDVVRDNSIQIRTSASTV
                     LEHLVVGAILAALVVLLFLGSLRSTVIAAIAIPVSIVSTFGLMMIAGFTLNMMTLLAL
                     ALAVGIVIDDAIVVLENIYRFIDEKRMKPFPAAIHATKEIGLAVLATTLSLMAVFLPV
                     AFMTGIVGRFLFSFGITMAFAVGVSVIVSFTIVPMLAARVLPPATGEHHRSWFQRVVD
                     AAYSPVERAYMAILRFAMRQRWLIVLACLAALATVPALGKKA"
     gene            complement(1657..>2352)
                     /locus_tag="VFV99_27060"
     CDS             complement(1657..>2352)
                     /locus_tag="VFV99_27060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5063076.1"
                     /translation="ERTKSLLDKGAITKSEYDRQMTQCTSAQEQVAAVEARQQMMSKS
                     VTDGLVRAPFDGVISEKMVSPGEWVAPGKPLFTLVDDDPLKVELSISEIAVPLIKKDQ
                     HVELFSVAKGEKSVGATITRLGAEIGRTRSLVVEAVIDKDSGLVPGMFVEGHIKIGEV
                     PRIIAPKDAVVKRGKTWRAFVVKKGEVEERVVQLGPSPAEGQVSISQGLVKGDKIVTK
                     VPDDLVDGRAVVE"
BASE COUNT          411 a          789 c          756 g          396 t
ORIGIN      
        1 ggccttcttg ccgagcgcgg gcacggtcgc gagcgcggcc aagcaggcca gcacgatcag
       61 ccagcgctgc cgcatcgcaa agcgcaggat cgccatgtaa gcgcgctcga ccggggagta
      121 ggccgcatcg acgacgcgct gaaaccagct gcgatgatgc tcgccagtgg ccggcggcag
      181 cacccgggcc gcgagcatcg ggacgatcgt gaagctgacg atcaccgaca cgccgaccgc
      241 gaacgccatg gtgatgccga agctgaacaa gaagcggccg acgatcccgg tcatgaacgc
      301 gaccggcaag aacaccgcca tcagcgacag cgtggtcgcg agcacggcga ggccgatttc
      361 cttcgtcgca tgaatggcgg cggggaacgg cttcatccgc ttctcgtcga tgaagcgata
      421 gatgttttcg agcacgacga tcgcatcgtc gatcacgatg ccgaccgcga gcgcgagcgc
      481 gagcagcgtc atcatgttga gcgtgaagcc ggcgatcatc atcagaccga acgtcgagac
      541 gatcgagacc ggaatcgcga tcgcggcgat caccgtcgag cgcaggctgc cgaggaacag
      601 cagcacgacc agcgcagcga ggatcgcgcc gacgacgagg tgctcgagga ccgtcgacgc
      661 cgacgtacgg atctggatcg agttgtcgcg cacgacgtcg accgagtaat cgggcggcag
      721 ctgcttcttg agctcctgga ccgcgttcgt caccgcgtcg accacggcga cggtgttcgt
      781 gcctgtctgc ttacggacgg acagcgcgat cgccggcacg ccattgcgga gcgcggccgt
      841 ttcggcctct tcctcggcgt cctcgaggtc tgccacgtcg cgaacgcgga ccgcgtggtc
      901 gccctgctgg cggacgacga tgtcgccgat ctgcttcgcc tcgatcgcgc gcccttcgat
      961 gcgcagcgtc gagttcttcg gccccatctc gaactgaccg ccgggaacgt tgacgtttgc
     1021 gccggcgatc gcgcgctgca cttcgagcgc ggagacgccg gtcgcagcga ggcggatcga
     1081 atcgaggttg acgttgatct gacggtcctg gccgccgagg atcacgatct gaccgacgcc
     1141 cttgaggcgc tcgatgcgct gcttgaccga cttgtcggca aaccgcgtga ggtcacgcgt
     1201 cgacatgccc ttcggtcctt tgaccgacag cacgatgacc ggtgcggcgt caggatccgc
     1261 gcggcgcact tcggggcgca cgccgggcgg caggtcgcgc aacaccgtcg cgatgtgctc
     1321 gctgacgtcg tcggcggcat ccttcgggtc gatctcgagc tgaaactgcg cgagcacgag
     1381 cgacacgccc tcggtcgaga ccgaggtcaa cgtgtcgagg ccgttcaccg tgttgaccgc
     1441 ttcttcgatc ttctggctga cgtcggtctc gacggcaccg ggcgacgcgc ccgggtacgg
     1501 cgtcacgatc atcaccatcg gaaagtcgac cttggggaac ttgtcgacgc cgaggctctt
     1561 gtagccgacg atgccgacga cgacgaacac cgcgatcaac acgctcgcga agatcggtcg
     1621 cctgacgcag acgtttgcta accactgcac ggcgctttac tccaccaccg cgcgtccatc
     1681 gacgaggtcg tcggggacct tggtgacgat cttgtcgccc ttcaccagac cctgagaaat
     1741 cgacacctgg ccttcggccg gcgacggtcc gagctgcacg acgcgctcct cgacctcgcc
     1801 cttcttgacc acgaatgcgc gccaggtctt gccgcgcttc acgacggcat ccttgggcgc
     1861 gatgatgcgc ggcacctcgc caatcttgat gtggccctcg acgaacatgc ccggcacgag
     1921 gccgctgtcc ttgtcgatga ccgcctcgac gacgagcgac cgcgtgcggc cgatttcagc
     1981 gccgagccgc gtgatcgtgg cgccgacgga cttctcgcct ttcgcgacgc tgaacagctc
     2041 gacgtgctgg tctttcttga tcagcggcac cgcgatctcg gagatcgaga gctcgacctt
     2101 cagcggatcg tcgtcgacga gcgtgaacag cggcttgcca ggggcaaccc actcgccggg
     2161 cgacaccatc ttctcggaga tcacgccgtc aaacggcgca cgcacgagac catcggtgac
     2221 cgacttcgac atcatctgct ggcgcgcttc gacagcggcg acctgctcct gcgccgaggt
     2281 gcactgagtc atctggcggt cgtattcgga cttcgtgatc gcacccttgt cgagcagcga
     2341 cttcgtgcgc tc
//
LOCUS       DASUFX010000669         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1775016, whole genome shotgun sequence.
ACCESSION   DASUFX010000669 DASUFX010000000
VERSION     DASUFX010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775016"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFV99_27065"
     CDS             <1..387
                     /locus_tag="VFV99_27065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063077.1"
                     /translation="VLVAIPKSGGAARTIAKGFVRATALAADDTNAYVYTEKDGTLRK
                     VELASGATSVLATQLGNADDLVSEGEWLYTVSWPSKLVRIAKDGSRTDVLADDLKSPT
                     HIALDPAFVYVVSRDQNKIVRLRKAR"
     gene            476..1156
                     /locus_tag="VFV99_27070"
     CDS             476..1156
                     /locus_tag="VFV99_27070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5063078.1"
                     /translation="MSLLDEHKAERRARIGKVARQLVVERGYAGLTMRELAQKARVAV
                     PTLYNLFGSKDAILVAELAESARVLAASLPPIGDSFFARAMVGFDAGTRAIEESPEFY
                     RECMNMFLTSPETAEMRRRVEDAHIAVMTANLRAAKAAGQLADWATPEIVARHMFAQY
                     MATLIAWGVGEIDLPTFRIAALSGVCHLLAGAARGPFAAEVEAKLRTLPTQYTHSPPD
                     KKATRRST"
     gene            1153..2250
                     /locus_tag="VFV99_27075"
     CDS             1153..2250
                     /locus_tag="VFV99_27075"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5063079.1"
                     /translation="MMIDDVELSAPKPGHNLPPRAFTSSALFEREQHAIFAKSWVHVA
                     DVIDLPGPGSYVAGLIGRTPVLLVRDRHTGELRGFLNACRHRGAQLLDGKGTCEKQIK
                     CPYHAWSYGTDGKLLGVPYRDEFSCDVSQMGLVPIRVGSVGPLVFACLDADAPPLADW
                     VATLPAALAAAGVDNWALAFELRYEIKANWKLFVENANDGYHIQFVHDVLTDLLQADS
                     GVTVLEPHSAYSYANVDPKYVPPGFEAADAKIRFGCIFPNLIPVLSPQDLTYMRVDPI
                     SHDRLALFVRSYDNAEFAHLRDFRKAAFQRTTDQDIAVVERTHRGLNAIGLPAGVHAS
                     RLEERIGHFERMWADAMTDEPAPKRHLAIAP"
     gene            2295..2699
                     /locus_tag="VFV99_27080"
     CDS             2295..2699
                     /locus_tag="VFV99_27080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5063080.1"
                     /translation="MAHGATLTRLLRARDLLHADSERGPTLDELAGAAGLSRAHLARQ
                     FAQTFGVPPHQYLVQLRLDRAKRALASGESVTDVCYQVGFESLGTFSASFRRRTGMSP
                     REWQRAARPFVQSRGVPVLYIPGCFLIHAEHV"
     gene            2737..3144
                     /locus_tag="VFV99_27085"
     CDS             2737..3144
                     /locus_tag="VFV99_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5063081.1"
                     /translation="MITKQSHATIYVTDQDRAKAFYTEKLGFEVRDDARMGPFRWLTV
                     GPKTQPDLNIILYGIQPSQFMSEENAALLKKLVESGAIGGGVLECDDIRKTYEQLKAK
                     GVEFKGEPKEQPYGIEAVFKDDSGNYWSLGQKR"
BASE COUNT          576 a         1089 c         1032 g          550 t
ORIGIN      
        1 gtgctcgtcg cgattccgaa gagcggcggc gctgcgcgga cgatcgcgaa gggcttcgtg
       61 cgtgcgaccg cgctcgcggc cgacgacacc aacgcatacg tctacaccga gaaagatgga
      121 acgctacgca aggtcgagct cgcgagcggc gcgacgagcg tgctcgcaac ccagctcggc
      181 aacgcggacg atctcgtcag cgaaggcgag tggctctaca cggtgtcgtg gccgagcaag
      241 ctcgttcgca ttgccaagga tggctcgcgc actgatgtgc ttgccgacga cctcaagtcg
      301 ccgacccata tcgcgctcga tccggcgttc gtctacgtcg tcagccgcga ccagaacaag
      361 atcgtccggc tgcgaaaagc gcgctaacaa ccggtcgtca ttggcggaat catgacgctg
      421 caggaaatct gcttgccaaa aatgaaacgc gttataagat ttaaacacgt ttcaaatgag
      481 tctcctggac gagcacaaag ccgaacgtcg cgcccgcatc ggcaaggtcg cgcgccagct
      541 cgtcgtcgag cgcggctacg ccggcctcac gatgcgcgag ctcgcccaga aggcacgggt
      601 cgcggtgccg acgctctaca acctgttcgg tagcaaggac gcgatcctgg tcgccgagct
      661 cgccgagtcc gcccgcgtgc tcgcggcatc gctgccgccg atcggcgaca gcttctttgc
      721 gcgcgcgatg gtcggcttcg atgcgggcac gcgcgcgatc gaggagtcgc ccgagttcta
      781 ccgcgagtgc atgaacatgt tcctgacctc gcccgagact gccgagatgc gccgccgcgt
      841 cgaggacgcg cacatcgcgg tgatgacggc caacctgcgc gcggcgaagg cggccgggca
      901 gctcgccgac tgggcgacgc ccgagatcgt cgcgcgccac atgttcgcgc agtacatggc
      961 cacgttgatc gcgtggggtg tcggcgaaat cgacctgccg acattccgta tcgcggcact
     1021 gtccggcgtt tgtcacctgc ttgccggcgc cgcgcgcgga ccgttcgcgg ccgaggtcga
     1081 agccaagctc cgcacgctgc ccacgcagta cacgcattcc ccgcctgaca agaaggcgac
     1141 gaggaggtcc acatgatgat cgatgacgtc gagctctctg cacctaaacc cggccacaat
     1201 ctgccgccgc gcgcattcac gtccagcgcg ctgttcgagc gcgagcagca cgcgatcttc
     1261 gcgaagagct gggtccacgt tgctgacgtg atcgatttgc ccgggccggg cagctatgtc
     1321 gcgggactga tcggccgcac gccggttcta ctcgtgcgcg atcgccacac gggcgagctg
     1381 cgcgggttct tgaacgcgtg tcgtcaccgt ggcgcgcagc tgctcgacgg caagggcacc
     1441 tgcgagaagc agatcaagtg cccgtaccac gcgtggagct acggcaccga cggcaagctg
     1501 ctcggtgtgc cgtatcgcga cgagttctcg tgcgacgttt cgcagatggg tctcgtgccg
     1561 attcgcgtcg gcagcgtcgg gccgctcgtg ttcgcctgcc tcgacgctga tgcaccgccg
     1621 ctcgccgact gggttgcgac gctacccgcc gcgctcgccg ccgcgggcgt cgacaactgg
     1681 gcgctcgcgt tcgagctgcg ctacgagatc aaggccaact ggaagctgtt cgtcgagaac
     1741 gcgaacgatg gctatcacat ccagttcgtc cacgacgtac tcaccgatct gctgcaagcc
     1801 gactccggtg tcaccgtgct cgagccgcac agcgcgtact cgtacgcgaa cgtcgatccg
     1861 aagtacgtgc cacccggctt cgaagcggcg gacgcgaaga ttcgcttcgg ctgcatcttc
     1921 ccgaacctga tcccggtgct gtcgccgcag gacctgacgt acatgcgcgt cgatccgatc
     1981 tcgcacgatc gactcgcgct gttcgtgcgc tcgtatgaca acgccgagtt cgcgcacctg
     2041 cgcgacttcc gcaaggccgc gttccagcgc acgaccgatc aagacatcgc ggtcgtcgag
     2101 cgcacgcatc gtggcctcaa cgcgatcggc ctgccggcag gtgtccacgc atcacggctc
     2161 gaagagcgca tcggtcactt cgaacgcatg tgggccgatg cgatgaccga cgagcccgca
     2221 ccgaagcgcc acctggcgat cgctccctga agttcgcgcg tgaccgccgc cgcggttcgg
     2281 gctatctctg gtccatggcc cacggcgcga cgctcacgcg actgcttcgc gcgcgcgatc
     2341 tgcttcacgc ggattccgag cgcgggccga cgctcgacga gctcgccggt gccgctggcc
     2401 tctcgcgcgc ccacctcgcg cgccagttcg cgcagacgtt cggcgtgccg ccgcaccaat
     2461 atctcgtcca gctgcgcctc gatcgcgcga agcgtgcgct cgcgtcgggc gagagcgtca
     2521 ccgacgtctg ctaccaggtc gggttcgaga gcctcggcac gttctcggcg agctttcgcc
     2581 gtcgcacggg catgtcgcca cgggagtggc agcgcgcggc gcggccgttc gtgcaaagcc
     2641 gcggcgttcc agtgctctac atccccggct gctttctgat tcacgccgag cacgtttgaa
     2701 gaagttcgcg gcgcgggcct gcgctacgaa cgcttcatga tcaccaagca atcgcacgcc
     2761 actatctacg tcaccgacca agatcgcgcg aaggcgttct ataccgagaa gctcggcttc
     2821 gaagttcgcg acgacgcccg catggggcca ttccgctggc tgaccgttgg cccgaagacg
     2881 cagccagacc tcaacatcat tctatatggc atccagccga gtcagttcat gagcgaggag
     2941 aacgcggcgt tgctgaagaa gctcgtcgaa tccggcgcga tcggcggcgg cgtcctcgag
     3001 tgcgacgaca ttcgcaagac gtacgagcag ctcaaggcga agggcgtcga gttcaagggt
     3061 gagccgaagg agcagccgta cgggatcgag gccgtgttca aggacgactc gggcaactac
     3121 tggagcctcg gccagaagcg ttagttcttg tcgaccggac gctagttctt gttcggatcg
     3181 agaaacgcct gcttgccctt gtagtcgagc acgagcacga actgattgaa cacgaggctg
     3241 ccgatca
//
LOCUS       DASUFX010000670         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_385177,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000670 DASUFX010000000
VERSION     DASUFX010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385177"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /locus_tag="VFV99_27090"
     CDS             <1..2267
                     /locus_tag="VFV99_27090"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX5063082.1"
                     /translation="NSGGAGTTWCRELEHSTQVGVTHGTLKIRDDSAVPASTQIALDA
                     ARNEYEPFQIVINGGAAGISDVQVTKTALVGDAGTIDATQIRLFQIGRYNVRWASSAE
                     GASGYWPDPLIPDVDAYDNEPRNAFVHFNIAANQTRAVWVDVRVPADQPAGTYQGVFM
                     VSGTNLAPTPINVRLRVRNFTLPSTATLKTAFGLNGDAVTRGHLGGQWCSDFPAPQVN
                     ELLGKYARAALDHRITLMNPTCGDPAASFAQFDMYQGTLLDGTAATELAGARSQFFRS
                     SRGDAAGMTALRSHFDVHGWGNTLVDYSCDEPPQACDLANWPARAQRAHDAGIPNLVT
                     TSYEYLQQHGWLDLVDILVPVAETMNETSRAQFDEFLSRGPTKQLWWYQSCDSHGCGG
                     CDAGMSAFNQYNGLPSYVIDSDARQNRAMEWLSFIFDVEGELYWETAFNLQAGTDWDS
                     GTSYCGFGGSGDGVMFYPGKPTDPRVGGTTQIPLESVRMKLIREGMEDYEYLVLYAQR
                     FGRDAAVAKAKQVFPDVFGSKQHPAELLYQVRRELADGIEGVAETPVDVPHAPELVDL
                     AGDLHEFAASPPIAVAAGAANAEFRLLWDENNLYIAATVTDADLRTQGSGRDGPLYNS
                     DSVEVMLDPQLTQTPMATSAVMQVIASVAGDLYDARGAGSAGDATFDIAGLDARAVIH
                     GTLDDAVGDTGYQIIIAIPWQSPVAADQMLGVDLALDNLSASSLTFDDWAGVRPFAQP
                     RYWHQVRLASDMCR"
     gene            2370..>2730
                     /locus_tag="VFV99_27095"
     CDS             2370..>2730
                     /locus_tag="VFV99_27095"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5063083.1"
                     /translation="MRRIFLWLPLCLAACDHTVDDQKPIGGHTDSEAEVQRYVRRAYL
                     DLTGRPPSDADLQATTVRLRDAGNTAAARASFVDERIASDDYAKLWVEELENGIFGGN
                     TLSQQYAFVCSIVRGQDR"
BASE COUNT          485 a          902 c          889 g          454 t
ORIGIN      
        1 gaaacagcgg cggcgcggga acgacgtggt gcagggagct ggagcattcg acgcaggtcg
       61 gtgtcacgca cggcacgctg aagattcgtg acgattcggc ggtgccggcg agcacacaga
      121 tcgcgctcga tgctgcgcgc aacgagtacg agccgtttca gatcgtgatc aacggcggcg
      181 ctgccggtat ttccgatgtt caggtgacca agacggcgct cgttggcgac gccggcacga
      241 tcgacgcgac acagatccgg ctattccaga tcgggcgcta taacgtgcgc tgggcgtcga
      301 gcgccgaggg cgcgagcggc tactggcccg atccattgat ccccgatgtc gacgcgtacg
      361 acaacgagcc gcgcaacgcg ttcgtgcact tcaacatcgc cgcgaatcag acgcgcgcgg
      421 tgtgggtcga cgttcgcgtg ccggccgatc agcccgcggg cacgtatcaa ggcgtgttca
      481 tggtgagcgg cacgaacctc gcgccgacgc cgatcaacgt gcgcctgcgc gtgcgcaact
      541 tcacgctgcc gtcgactgcg acgctgaaga ctgcgttcgg cctcaacggc gacgcggtca
      601 cgcgcggcca cctcggcggc cagtggtgca gcgacttccc ggccccgcag gtcaacgagc
      661 tgctcggcaa gtacgcgcgc gctgcgctcg atcatcggat cacgctgatg aatccgacgt
      721 gcggcgatcc ggccgcgagc ttcgcgcagt tcgacatgta tcaaggcacg ctgctcgacg
      781 gcacggcagc caccgagctc gccggtgcgc gctcgcagtt cttcaggagc tcgcgcggcg
      841 acgccgcggg catgaccgcg ctcaggtcac acttcgacgt ccacggctgg ggcaacacgc
      901 tcgtcgatta ctcgtgcgac gagccgccgc aagcgtgtga cctcgcaaac tggccagcac
      961 gcgctcagcg cgcacacgac gccggcattc cgaacctcgt cacgacgtcg tacgagtacc
     1021 tccagcagca cggctggctc gatctcgtcg acatcctcgt gccggtcgcc gagacaatga
     1081 acgagacgag ccgcgcacag ttcgacgagt ttctgtcacg cggtcccacg aagcagctgt
     1141 ggtggtacca gtcgtgtgac tcgcacggct gcggcggctg cgatgccggg atgagcgcgt
     1201 tcaatcagta caacggcctg ccgtcgtacg tgatcgactc cgacgcgcga cagaaccgcg
     1261 cgatggagtg gctgtcgttc atcttcgacg tcgagggcga gctctactgg gagaccgcgt
     1321 tcaacttgca ggccggcacc gattgggatt ccggcacctc gtactgtggc ttcggcggca
     1381 gcggcgacgg cgtcatgttc tatccgggca agccgaccga tccgcgcgtc ggtggcacca
     1441 cgcagatccc actcgagtcg gtgcgcatga agctgatccg cgaaggcatg gaggattacg
     1501 agtacctcgt gctgtacgcg cagaggttcg gccgcgacgc cgcggttgcg aaggcgaagc
     1561 aggtgttccc ggacgtcttc gggagcaaac agcatcccgc cgagctgctc tatcaagtgc
     1621 gccgcgagct cgcggatggc atcgaaggcg tcgccgaaac gccggtcgac gttccgcacg
     1681 ctcccgagct cgtcgatctc gcgggcgacc tccacgagtt cgccgcatcg cccccgatcg
     1741 cagtcgcagc cggcgccgcg aacgccgagt tccgcctgct atgggacgag aacaacctct
     1801 acatcgccgc gaccgtcacc gacgccgacc tgcgcacgca gggcagcggc cgcgatggcc
     1861 cgctctataa ctccgacagc gtcgaggtga tgctcgatcc gcagctcacg cagaccccga
     1921 tggcaacgag cgccgtcatg caagtgatcg cgagcgtcgc tggcgatctc tacgacgcgc
     1981 gtggcgccgg ctcggccggt gacgcgacat tcgacatcgc cggcctcgat gcgcgcgcgg
     2041 tcatccacgg cacgctcgac gatgcggtcg gtgataccgg ctatcagatc atcatcgcga
     2101 tcccgtggca gtcaccggtc gcggcggatc aaatgctcgg cgtcgatctc gcactcgaca
     2161 atttgagcgc gagcagcctt acgttcgacg actgggcagg cgtgcggccg ttcgcgcagc
     2221 cgcgttactg gcatcaggtc cgactcgcat ccgacatgtg ccgctgatgg attactcggc
     2281 ggtgtaggaa caagccacac gctaactctc cggaaggttt tgcgcgacgc ctggtcttgg
     2341 cgttgcagaa tgtttccgtc tggagggata tgcgtcgcat tttcctatgg ctaccgctgt
     2401 gcctcgcggc ttgcgatcac acggtcgatg atcaaaagcc gatcggtggt cacaccgata
     2461 gcgaggccga ggttcagcgc tacgttcgcc gcgcgtacct cgacttgacc ggccgaccac
     2521 cgagtgatgc cgacctgcaa gcgaccaccg tgcgcttgcg cgatgccggc aacacagccg
     2581 cagcgcgcgc gtcgttcgtc gacgaacgga tcgcgagcga tgactacgcc aagttgtggg
     2641 tcgaagagct cgagaacggg atcttcggcg gcaacacgct gagtcagcag tacgcgttcg
     2701 tctgctcgat cgtgcgcggc caggaccgca
//
LOCUS       DASUFX010000671         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1080088, whole genome shotgun sequence.
ACCESSION   DASUFX010000671 DASUFX010000000
VERSION     DASUFX010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080088"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1883
                     /locus_tag="VFV99_27100"
     CDS             249..1883
                     /locus_tag="VFV99_27100"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5063084.1"
                     /translation="MKPSVLVVSLGVCVSGSAFAGGLILPGSGAVSTSRAGTGVASTE
                     GGEAISLNPAGMAKSKGTTITVGAAIINYAMSFQRNGTYDTYTDPNLDPTPYEGQDYP
                     IVENNPKPPLGIGPYQPIPVIAVTTDLGGRVPGLTVGAGLYAPNAYPFRNMNNVNGQP
                     FFVPKSNGGYSFPTNLDAPPPPTRYDIIEQEAAIILPSVAASYRIMPDLDVGARFSIG
                     WSEVKSTVAVWGEPANYNEYIKKDGLFTLDAKDSFVTAWALGAAYRPAPTIELGAQYT
                     GQIDMRAEGEAVNENGPQVTLNMQPIMVLPSIGSRCNAGGTPEKLKGCVEFAVPMTAT
                     VGGRYKFLGEGGKLNGDLELNVNWQHWGAERTQDYRVVVDAQVTTASMPDNGIDLKDN
                     IVRHGLKDTYGVLLGGSWNFPAGNNTVTARGGVGYETGAAKKGWERADFDGAARAIFT
                     VGGSYKLPRVSIDLGFGYIYEGTRTDSRTCNPVLTAPPYEGCGPNGEQQPIEDRQGPG
                     PINPIVNADQQLENPVNEGTYKSHYLLFMLGMSTWF"
     gene            1893..2165
                     /locus_tag="VFV99_27105"
     CDS             1893..2165
                     /locus_tag="VFV99_27105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063085.1"
                     /translation="MSKALIKRGSGGGDPASGIVPAVASAIIPGVGQLINGETDKAIG
                     VFVTATVCGASLVLSLPLIGTVAAGVGAVTWLYGVADGYFTGKKKG"
     gene            complement(2162..3016)
                     /locus_tag="VFV99_27110"
     CDS             complement(2162..3016)
                     /locus_tag="VFV99_27110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063086.1"
                     /translation="MDRPEVTFTTRSAWYSRGSTVIGTSRLTLPVDVTAYISRAGAAH
                     GYWRNAPPLPSYFGYCDVPALMPILIGAHTRRAITQHDAHALEADGDEPRGPIELHIR
                     AGDVETSTKLDHDDEQRVIDDQRAIHHALAADYAEVLASWQRAADELGGSVATPWPPV
                     LTVPRGFGTTTILLTWPTTEQSAASASIELIADARKAKLWTLEREPLATPNTIAIADR
                     PFLLIGEIPLAMDHLDRLVRRAEIMSITVRRHITVRIAGHEPTPDVFDALLDLLGELC
                     GTPSEPYR"
     gene            3067..3888
                     /locus_tag="VFV99_27115"
     CDS             3067..3888
                     /locus_tag="VFV99_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5063087.1"
                     /translation="MDEQFDQFKGTKTYIASEELRHAVNVAVALGRPLLVRGEPGTGK
                     TLLAENLSASLGLPLIRWHVKSTTKAKDGLYVYDTVARLHDSRFGGDVRDIAKYIKLG
                     PLGEALASPSRVVLLIDEIDKADIEFPNDLLLELDAMRFRIDETGREVVAAERPVVII
                     TSNNEKELPDAFLRRCVFHYIQFPDRELMAEIVRVHHPDITDRVLDNALEMFFSLRST
                     PKLRKKPSTSELIDWICALKKAGVDLSKVGHGIPFLGTLLKTESDVELVAKRAKA"
     gene            3889..5112
                     /locus_tag="VFV99_27120"
     CDS             3889..5112
                     /locus_tag="VFV99_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5063088.1"
                     /translation="MLIDFLFELRRRKIPVSTHEWMALMEAMALGLHESSLDGFYHLC
                     RTLCVKDIAFYDQYDEAFLAYFKDVHTDALKLTEQLASWLMDPKMLEGLTEEQRKALQ
                     ELDLEKLRELFEKRLKEQKERHDGGNRWIGTGGTSPFGTRGMNPNGMRVGGGGGRSAM
                     AVADQRLFKEYRRDVVLDVRQIDVALRGLRRLGREGAHEELDLDETVDETCKNAGELE
                     IIFRPPRRNRVKVLLLMDVGGSMDPHSELMSRLFTAASRTGRFAKFRSYYFHNCVYNS
                     VYEDAQFRKGVPVADLLSHSDRDEKLVMVGDALMHPAELLDPGGSMYLYSQTRASGIE
                     WLRRLAHHFRSSAWLNPEPDRFWSGTTIEVIASVLPMFPLTLDGLSHAVRYLVRGGEK
                     PRVGDPSKWLKAMGS"
     gene            complement(5148..5678)
                     /locus_tag="VFV99_27125"
     CDS             complement(5148..5678)
                     /locus_tag="VFV99_27125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063089.1"
                     /translation="MRLLLRVVMPACLLVAGCKDKQQTPAPATAPKPAPTQPAQKPSL
                     PTTETPATSAAPTAPAEQPAPPGKPITVAEATAALPTITSPQLIALKQTSNQEQVHGT
                     WCIDGTSADDVAKQVGHLLAQAKYTGISIRGDAKKAGVSGDREGVRFSMVVSASSAAV
                     CAAPAHYFASATIFKI"
     gene            complement(5678..7630)
                     /locus_tag="VFV99_27130"
     CDS             complement(5678..7630)
                     /locus_tag="VFV99_27130"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEX5063090.1"
                     /translation="MADPNTRQGKRTPVTLKIKFKSETLEQFIERYAVDVSQGGIFIR
                     TKEPLAVGTQMKFEFQLRDASPLIAGEGTVVWTRENDPSRPAIAPGMGVRFDRLADGS
                     QTVLERILAEKAKQAPQRSGNEGPTKPPLFTDTPTRVAPAPVQDALGVGSNRRPKSDS
                     EQTPLPPPRPFHSDADDFDEKAFEEATKVRSLDELAAQTAGLVGAEASDDAPTNTAIS
                     MPPDELAARRQAGRPDSLGEPAAPPAAASGANVDRESVPNLPEPPKATRSKPPLEAPS
                     EDQPAPRTRLGVQPAKAQTRAASAAPEAVGPEPSIPHAPSRPSAAPIIILLLVLVAGA
                     AGAVWWFVLRPEATENVAQKDPGSNGSAAVATNPANGSDGSNGSAGSAEQLAQNGSDG
                     SAGSAAVEAPKGPIVDTVIASSVSKGAIVAIADTDQKGPAPFTAKLEKDKVYKAIVSA
                     PGFTTQELDVKGGQDKLTAKLVAKKHVLSVTTDPPGADIYIDSVNTQKITPSDIELTA
                     AQAAKPRVRVSLRRPGYRPIDQVVDAAMFKDNDAQMTASLNAKLTVMQRPTGGGSGGN
                     TGGNSSTTNTGSGTTSGSGSGSAATPPTGGGTGSAATPPTGGGTGSTTPPSGGGGNGT
                     GSAAPTTPGIPPATPGIPQSTPGVPE"
     gene            7751..>8004
                     /locus_tag="VFV99_27135"
     CDS             7751..>8004
                     /locus_tag="VFV99_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5063091.1"
                     /translation="MSTPIRRVLVANRGEIAVRVMRTCRERGLETVAVYSDADRLAPH
                     VQIADAAVHIGPPPSAQSYLVIEKIIAACKQTGADAVHPG"
BASE COUNT         1277 a         2585 c         2753 g         1389 t
ORIGIN      
        1 ggggcacttc gaagcgaacg gacacccgtc tataggcgaa ggggatctcg aggtgcacgg
       61 cacattcgtt gcgcgcccgt gcagccgtat tcacgactga agcgagccag tcggcgcgta
      121 ctccacgcac aggatgcgca gaagcgaggt ggcgcactgc aaccggcgta agtgaaccct
      181 gaccacttca ctagccacac gtaatcgctg gaagaagcgg cgctcactgg gataccgttc
      241 gcttccatat gaagcctagc gtcctggtgg tctcgctcgg agtctgcgtt tcgggttccg
      301 cattcgccgg cggattgatc cttcccggtt caggtgcagt ctcgacctcg cgcgccggca
      361 ctggcgtcgc gtcgacggaa ggcggcgagg caatctcgct gaaccccgcc ggcatggcga
      421 agtcgaaggg cacgacgatc accgtcggtg ccgcgatcat caactacgcg atgtcgttcc
      481 agcgcaacgg cacgtacgac acgtacacgg atccgaatct cgatccgacg ccgtacgaag
      541 gccaggacta tccgatcgtc gagaacaatc cgaagccgcc gctcggcatc gggccgtatc
      601 agccgattcc ggtgatcgcg gtgacgacgg atctcggcgg acgcgtgccg ggcttgacgg
      661 tcggtgcggg tctctacgcg ccgaacgcgt atccgttccg caacatgaac aacgtcaacg
      721 gccagccgtt cttcgtgccg aagagcaacg gcggctatag cttcccgacg aacctcgacg
      781 cgccgccgcc gccaactcgc tacgacatca tcgagcagga agccgcgatc atcttgccga
      841 gcgtcgcggc gtcgtatcgc atcatgccgg acctcgacgt cggtgcacgc ttctcgatcg
      901 gctggtcgga agtgaagtcg accgtcgcgg tgtggggcga gccggcgaac tacaacgagt
      961 acatcaagaa ggatggcctg ttcacgctcg acgcgaagga cagcttcgtc accgcgtggg
     1021 cactcggtgc cgcgtatcgg ccggcgccga cgatcgagct cggcgcgcag tacaccgggc
     1081 agatcgacat gcgggccgaa ggcgaagcgg tcaacgagaa cggcccgcag gtcacgctca
     1141 acatgcagcc gatcatggtg ctgccgagca tcggcagccg ctgcaatgcg ggcggtacgc
     1201 cggagaagct caaggggtgt gtcgagttcg cggtaccgat gaccgcgacg gtcggcggcc
     1261 gttacaagtt cctcggtgag gggggcaaac tgaacggcga cctcgagctc aacgtcaact
     1321 ggcagcactg gggcgccgag cgcacgcagg actatcgcgt tgtcgtcgac gcgcaggtca
     1381 cgacggcgag catgccggac aacggcatcg acctgaagga caacatcgtt cgccacggcc
     1441 tcaaggacac ctatggcgtg ctgcttggcg gcagctggaa cttcccggcc ggcaacaaca
     1501 ccgtgaccgc gcgtggtggc gtcggctacg agacgggcgc ggcgaagaag ggctgggagc
     1561 gcgccgattt cgatggcgca gcgcgcgcga tctttaccgt cggtggctcg tacaagctgc
     1621 cgcgcgtgtc gatcgatctc ggcttcggct acatctacga aggcacgcgg accgattcgc
     1681 gtacgtgcaa cccggtcctg actgcgccgc cgtacgaggg ctgtggcccc aacggcgagc
     1741 agcagcccat cgaggatcgc cagggtcccg gcccgatcaa cccgatcgtc aacgccgacc
     1801 aacagctgga gaatccggtc aacgagggta cgtacaagag tcactacttg ctgttcatgc
     1861 tgggtatgtc aacgtggttc tgaggtgagc tgatgagcaa ggcgctgatc aaacgcggaa
     1921 gtggtggtgg cgatccggcg tcgggaatcg tgccggcagt cgcgagcgcg atcattcccg
     1981 gtgtcggcca gctcatcaac ggcgagaccg acaaggcgat cggcgtgttt gtcaccgcga
     2041 ccgtgtgcgg tgcgagcctc gtgttgtcgt tgccgctgat cggcacggtc gcggcgggcg
     2101 tcggtgcagt gacgtggctg tacggcgtcg ccgacggcta cttcaccggc aagaaaaagg
     2161 gctagcgata cggttcgctc ggcgtgccgc agagctcgcc gagcagatcg agtagcgcat
     2221 cgaagacatc gggcgtcggc tcgtggccgg cgatgcgcac cgtgatgtga cggcgcaccg
     2281 tgatcgacat gatctcggcg cggcgcacga ggcggtcgag gtgatccatc gcgagtggga
     2341 tctcgccgat gagcaggaac gggcgatcgg cgatcgcgat cgtgttcggc gtcgcgagcg
     2401 gctcgcgctc gagggtccag agcttcgcct tgcgcgcatc ggcgatgagc tcgatcgatg
     2461 cggatgcggc gctctgctcg gtggtcggcc aggtcagtag gatcgtcgtc gtgccgaagc
     2521 cgcgcggcac ggtgagcacc ggtggccacg gcgttgcgac gctgccgccg agctcgtctg
     2581 cggcgcgctg ccagctcgcg aggacctcgg cgtagtcggc agcgagcgcg tgatggatcg
     2641 cgcgttgatc gtcgatcacg cgctgttcgt catcgtggtc gagcttcgtg ctggtctcga
     2701 cgtcgcccgc gcggatgtgg agctcgatcg ggccgcgcgg ttcgtcgccg tcggcttcga
     2761 gtgcgtgcgc gtcgtgttgc gtgatcgcgc gtcgcgtgtg cgcgccgatc aggatcggca
     2821 tcagtgcggg cacgtcgcag taaccgaagt acgacggcag gggaggggcg ttgcgccagt
     2881 agccatgcgc ggcgcctgcg cgcgagatgt aggccgtgac atcgaccggt aacgtgagcc
     2941 ggctcgtgcc gatcaccgtc gagccccgcg agtaccaggc ggatctcgtt gtgaacgtga
     3001 cttcgggccg gtccacctcg aaagcatagc cagacgagcc gggctgggat gctatggtcg
     3061 gcggaaatgg acgagcagtt cgatcagttc aaggggacca agacgtatat cgcctccgag
     3121 gagctgcgcc acgccgtgaa cgtggctgtg gcgctcggtc gcccgctgct ggtccgcggc
     3181 gagcccggca ccggcaagac gctgctcgcc gagaacctct ccgcctcgct cggcctgccg
     3241 ctcatccgct ggcacgtgaa gtcgacgacg aaggcgaagg acggcctcta cgtctacgac
     3301 acggtcgcgc gcctgcacga ctcgcggttc ggcggcgacg tccgcgacat cgcgaagtac
     3361 atcaagcttg gcccgctcgg cgaggcgctc gcgtcgccgt cgcgcgtcgt gttgctgatc
     3421 gacgagatcg acaaggccga catcgagttc ccgaacgacc tgttgctcga gctcgacgcg
     3481 atgcggttcc gcatcgacga gaccggccgc gaggtggtcg ccgcggagcg cccggtcgtc
     3541 atcatcacgt cgaacaacga gaaggagctg cccgacgcgt tcttgcgccg ctgcgtgttc
     3601 cactacatcc agtttccgga tcgcgagctg atggcggaga tcgtgcgcgt gcaccatccc
     3661 gacatcaccg atcgcgtgct cgacaacgcg ctcgagatgt tcttctcgct gcgctcgacg
     3721 ccgaagctgc gcaagaagcc gtcgacgtcg gagctgatcg actggatctg cgcgctgaag
     3781 aaggccggcg tcgatctgtc gaaggtcggc cacggcattc cgttcctcgg cacgctgctc
     3841 aagaccgaga gcgacgtgga gctcgtcgca aagcgagcga aagcgtagat gctgatcgac
     3901 ttcctcttcg agcttcgccg ccggaagatt ccggtgtcga cgcacgagtg gatggcgctc
     3961 atggaggcga tggcgctcgg tctgcacgag tcctcgctcg acggcttcta tcacctgtgt
     4021 cgcacgctct gcgtgaagga catcgcgttc tacgatcagt acgacgaggc attcctcgcg
     4081 tacttcaagg acgtccatac cgacgcgctc aagctcaccg agcagctcgc gtcgtggctc
     4141 atggatccga agatgctcga gggactcacc gaggagcagc gcaaggcgct ccaggagctc
     4201 gatctcgaga agctgcgcga gctgttcgag aagcggctga aggaacagaa ggaacgccac
     4261 gacggtggca atcgctggat cggtaccggc ggcacgtctc cgttcggcac gcgcgggatg
     4321 aatccgaacg gcatgcgcgt cggcggcggt ggcggtcgca gcgcgatggc agtcgccgat
     4381 cagcggctgt tcaaggagta tcgtcgcgac gtcgtgctcg acgtccgcca gatcgacgtc
     4441 gcgctgcgcg gtctgcgccg gctcggtcgc gaaggcgcgc acgaggagct cgatctcgac
     4501 gagaccgtcg acgagacctg caagaacgcg ggcgagctcg agatcatctt ccggccgccg
     4561 cgccgcaacc gcgtcaaggt gctgctgctc atggacgtcg gcggctcgat ggatccgcac
     4621 agcgagctga tgagccggct gttcaccgca gcgtcgcgca ctgggcggtt cgcgaagttc
     4681 cgcagctact acttccacaa ctgcgtctac aactcggtct acgaggacgc gcagttccgc
     4741 aaaggcgtgc ccgtcgcgga cctcttgtcg cacagcgatc gcgacgagaa gctcgtcatg
     4801 gtcggcgatg cgctgatgca tccggcggag ctgctcgatc cgggcggctc gatgtacctg
     4861 tactcgcaga cgcgcgcgtc gggcatcgag tggctgcgcc ggctcgcgca tcacttccgc
     4921 agctctgcgt ggctgaaccc cgagccggac cggttctggt cgggcacgac gatcgaagtg
     4981 atcgcgagcg tgctgccgat gtttccgctc acgctcgacg ggctctcgca cgccgtgcgc
     5041 tatctcgtgc gcggcggtga gaagccgcgc gtcggtgacc cgagcaagtg gctcaaggcg
     5101 atgggcagct gacaggcgtc ggacctcggg cttcggtcag atccagatca gatcttgaag
     5161 atggtcgcgc tcgcgaagta gtgcgcgggc gcggcgcaca cggcagcgga cgacgccgac
     5221 acgaccatcg agaaccgcac gccttcgcga tcgcccgaca cgccggcctt cttcgcgtcg
     5281 ccgcggatcg agatgccggt gtacttcgcc tgcgcgagca ggtgaccgac ttgcttggcg
     5341 acatcatcgg cgctcgtgcc gtcgatgcac cacgtgccgt gcacctgctc ctgattcgac
     5401 gtctgcttga gcgcgatgag ctgcggcgac gtgatcgtgg gcagcgcggc ggtggcctcg
     5461 gcgaccgtga ttggcttacc gggaggagcc ggctgttcgg cgggcgcggt cggtgcggcc
     5521 gatgtggccg gcgtttcggt ggtcggcagg cttggcttct gcgccggttg cgtaggcgca
     5581 ggcttcggtg ccgtggcggg cgcaggcgtc tgctgtttgt ccttgcaccc ggcgaccagc
     5641 aggcacgctg gcatcacgac tcgcaacaac agacgcatta ctctgggacg ccgggagtcg
     5701 attgcgggat gcctggcgtc gccggcggaa taccaggagt cgtcggcgca gccgagcccg
     5761 tgccgttgcc gccgccaccg gaaggcggcg tggtcgagcc ggtgccgccg cccgtcggag
     5821 gtgtcgctgc cgagccggtg ccgccgcccg tcggaggtgt cgcggccgag cccgagccgc
     5881 taccactcgt cgtgccgctg cccgtgttcg tcgtgctgct gttgccgccg gtgttgccgc
     5941 cgctaccacc gccggtcggc ctctgcatga cggtgagctt cgcattcagg gacgcggtca
     6001 tctgcgcgtc gttgtccttg aacatcgccg cgtcgacgac ctgatcgatc ggccgatagc
     6061 ccggacggcg cagcgagacg cgtacgcgtg gcttggccgc ttgcgccgcg gtgagctcga
     6121 tgtccgacgg cgtgatcttc tgcgtgttga cgctgtcgat gtagatgtcg gcgcccggcg
     6181 gatcggtcgt gacgctgagg acgtgcttct tcgcgacgag cttcgcggtg agcttgtcct
     6241 ggccaccttt gacgtcgagc tcctgggtcg taaagcccgg cgcgctgacg atcgccttgt
     6301 agaccttgtc cttctcgagc ttcgcggtga acggggccgg gcccttctgg tcggtatccg
     6361 cgatcgcgac gatcgcgccc ttcgagacgc tcgatgcgat cacggtgtcg acgatcggcc
     6421 ccttcggcgc ctcgaccgct gcgctgcccg cgctaccgtc gctgccgttc tgcgcgagct
     6481 gctctgcgct gccggcgctg ccgttgctgc catcgctgcc attggccgga ttcgtcgcga
     6541 ccgcggcgct gccgttgcta cccggatcct tctgcgcgac gttctcggtc gcctcgggac
     6601 gcagcacgaa ccaccacacc gcgcctgccg cgcccgcgac gagcacgagc agcaggatga
     6661 tgatcggagc ggcgctcggc cgactcggag cgtgcggaat cgacggctcg ggccccaccg
     6721 cttcgggtgc ggcagaggcg gcgcgcgtct gcgctttcgc tggctgaacc ccgagccgcg
     6781 tgcgcggagc gggttggtct tcgctcggcg cctcgagcgg cggcttggag cgcgtcgcct
     6841 tcggcggctc gggaaggttt ggcaccgact cgcgatcgac gttcgccccc gacgcggccg
     6901 ccggcggagc agctggctcg ccgagcgaat cgggacggcc ggcctgccgg cgcgccgcga
     6961 gctcgtcggg cggcatcgag atcgcggtgt tcgtcggcgc gtcgtcgctg gcttcggcgc
     7021 cgacaagtcc cgccgtctgc gcggctagct cgtcgagcga gcggaccttc gtcgcttctt
     7081 cgaatgcctt ctcgtcgaag tcatccgcgt cggagtggaa cggacgtggc ggcggaagcg
     7141 gcgtctgctc ggaatccgac ttcgggcggc ggttgctacc gaccccgagt gcgtcttgca
     7201 ccggagccgg cgcaacgcgc gtgggtgtat cggtgaacag cggcggcttg gtcgggcctt
     7261 cgttgccgct gcgctgcggt gcctgcttcg ccttctcggc gagaatgcgc tcgagaacgg
     7321 tctgactgcc gtcggcgaga cgatcgaacc gcacgcccat gcccggtgcg attgccgggc
     7381 ggctcggatc gttctcgcgc gtccacacca ccgtgccttc gccggcgatc agtggagacg
     7441 cgtcacgaag ctgaaactcg aacttcatct gtgtaccaac ggccagtggc tccttggtcc
     7501 ggatgaagat gccgccctgg ctcacatcga ccgcgtagcg ctcgatgaat tgctcgaggg
     7561 tttcgctctt gaacttgatc ttgagcgtaa cgggcgtgcg cttaccctga cgggtgtttg
     7621 gatcggccaa gacgttctcc cgaggcgcgt tcgcagacag aggtcagcgc aactcgccga
     7681 ttcgcctcga ttctatgact ggtcgcccaa cgatgcaacg cgtcgccttg cagtagtaga
     7741 gtgcgcggcg atgtccaccc cgatccgtcg tgttctcgtc gccaatcgtg gcgaaatcgc
     7801 tgtccgggtc atgcgtacgt gtagggaacg cggactcgag accgtcgccg tctacagcga
     7861 cgccgaccgc ctcgcgcccc acgtccagat cgcggacgcc gcggtccaca tcggcccgcc
     7921 gccctccgcg cagagctacc tggtgatcga aaagatcatc gccgcctgca aacagaccgg
     7981 cgccgacgcg gtccatcccg ggta
//
LOCUS       DASUFX010000672         5601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_385404,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000672 DASUFX010000000
VERSION     DASUFX010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385404"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFV99_27140"
     CDS             complement(<1..779)
                     /locus_tag="VFV99_27140"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX5063092.1"
                     /translation="MIERWFGVRAAGSSLHVEVLGGATTFMTMAYILFVNGLVLSTAR
                     LPADQVLTVTALVAGVTTIAMGLVAKYPFALAPGMGINAAVASSLVATQGLTAPEAMG
                     VIVAEGLAITLLVVTGVRERVLDAIPDSLKRAIGAGIGLFLAVIGLTNGGVVVPSKGT
                     LLALGNLHSSHTIVFLVGLAIAFALTVRKVRGALLIAIAAATALAFALGDVHAPAKVV
                     ALPDFSLVGQFSFGFVAKLGALGAVIAVFSLMLSDFFDTMGT"
     gene            895..1917
                     /locus_tag="VFV99_27145"
     CDS             895..1917
                     /locus_tag="VFV99_27145"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX5063093.1"
                     /translation="MSVQLRSLRGELTAAGVFRTRELRSWLELAAMMSVLAMCLIGIA
                     LLGWIGALFLVPVAAVLCTSISMLGHEGSHRSFSASPARNAILCYLTFPLFGGLGALY
                     WRNKHDRLHHGHPNVEGMDPDIRPFPFSSSRGDHERCGRKTRWFQRHFQRRLFWPMSL
                     LMATGMRRSSVLYLIRYPKKREAGWWAEVLCMTAHYAGWVVVPSLIWGPLAGILVYVS
                     IWGLVGVCLALVFAPAHMGLPIVADQNHDWLHQLETTRNLQLPRVISFFFIGLDYQVE
                     HHLFPKIAHANLPQAAAITQAWCKRHGVTYQTMPYLDALGDAVRFMADAWTREASTAS
                     AMRMAS"
     gene            1982..3130
                     /locus_tag="VFV99_27150"
     CDS             1982..3130
                     /locus_tag="VFV99_27150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063094.1"
                     /translation="MELARGTIAERPWAVTLAMFARRCASGQLTLLAGDKRYSIAYDR
                     GSIVAARSPLIADSAARVALTSHIATPTHIRELERQQALIPGRDDVDLLAAIARLSPG
                     HINRLRADTIIRAAARTFAIDAGEYIFEDTACLPGRGVDVDVRAIIYHGIHMHLSDQR
                     LAADLRRLGGALFVLEDQASDELHKFGFCEGEWPLLAALREGASLPELEARHREIDPR
                     TMYSTVYALVACGTARVVAPSRLGTPPQFLRTKSNPELAAEAAERAERALKNEQLETA
                     VLELKTACELVPNNVDYNALLGWALFCASHDKHSAAVDAKKALERAVHRSDRPQVARF
                     YLGRIERMLGRDKEALRHFQAVLLEEPHNTDASAEVRVLQARMRQRAN"
     gene            complement(3123..4304)
                     /gene="cfa"
                     /locus_tag="VFV99_27155"
     CDS             complement(3123..4304)
                     /gene="cfa"
                     /locus_tag="VFV99_27155"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEX5063095.1"
                     /translation="METHSPAKSLIERFIPITLPTRSVEAESFVRELFGLAGIEIGGH
                     APGDIRVHDPRFYERVLRDASVGFGEAYMEEWWETDALDVTIDKIMRANLKQKITGSW
                     RMRALTVKAVMLNLQAKARSGASVEAHYDIGNDLYTRMLDQRMVYTCAYWDKAKTLQE
                     AQEHKLDLVCKKLGLEPGMKVLDLGCGWGGLASWAAEKYGCTVLGVTLSKDQVSLGNQ
                     LWKHLPVELRLSDYRDVKGTYDRVVSIGMMEHVGPKNHRTMMETIDRCLKPEGVALVH
                     TIANNRSLRHGTPFIEKYIFPNAVAPSLAQIGRAMEGLFVCEDLHNIGPDYDPTLMAW
                     WDNFDRTYSEISHRYDRKFYLMWKFYLLAAAGASRSRDGQLYQVVMTKTGRKQPYCRI
                     S"
     gene            4414..>5601
                     /locus_tag="VFV99_27160"
     CDS             4414..>5601
                     /locus_tag="VFV99_27160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX5063096.1"
                     /translation="MIKDLWYKNAIVYCVHVGAFMDSNGDGSGDFEGLMRRLDYLAGL
                     GVTCIWLMPFQESPQRDDGYDISDYYSVDDRYGSLGDFVDFTHEAKQRGIRVIIDLVV
                     NHTSDQHKWFQEARRDKHSRYRDYYIWSEKKPPEAEHGMVFPGVQKTTWTKDAVAGQY
                     YFHRFYDFQPDLNTHNPQVQEEIRKIMGFWLELGVSGFRMDAIPFVIQTKGADKKHTE
                     KYEMLHDMRDFLQWRTGDAIILAEANVTPDHNLNYFGKDGDRLQMMFNFWVNQRMFFA
                     LAGHDARPLRKALEQTKNRPDAAQWAMFLRNHDELDLGRLSDEERDIVFREMGAEKTM
                     QLYDRGIRRRLAPMLNGDRRRIELAYSVMFSLPGTPIMRYGDELGMGDNMRLKERNAV
                     RTPM"
BASE COUNT          994 a         1809 c         1792 g         1006 t
ORIGIN      
        1 gtgcccatcg tgtcgaagaa gtcggagagc atcagcgaga acactgcgat cacggcaccg
       61 agcgcaccga gcttcgcgac gaagccgaac gagaactgac cgacgaggga gaagtcgggc
      121 agggcgacga ccttcgctgg cgcgtggaca tcgccgagcg cgaatgcgag tgcggtcgct
      181 gccgcgatcg cgatgagcag tgcgccccgt accttgcgca cggtgagtgc gaacgcgatc
      241 gcgagaccga cgagaaatac gatcgtatgc gacgagtgca ggttgccgag cgcgagcaat
      301 gtgcccttgc tcggcacgac gacgccgccg ttggtaagcc cgatcaccgc gaggaacagg
      361 ccgatccccg caccgattgc gcgtttgagc gaatccggga tggcgtcgag cacgcgctcg
      421 cgcacgccgg tgacaacgag cagcgtgatc gcgagtcctt cggcgacgat gacgcccatc
      481 gcctcgggcg cggtcagacc ctgcgtcgct acgaggctgc tcgcaacggc tgcgttgatg
      541 cccatacccg gcgccagcgc gaatggatac ttcgcgacga ggcccatcgc gatcgtcgtg
      601 acgccggcga cgagtgcggt gacagtcagt acctggtccg ctggcagcct tgcggtgctg
      661 aggacgaggc cgttgacgaa caggatgtac gccatcgtca tgaacgtcgt tgccccgccc
      721 agaacctcga cgtgcagcga cgagcccgcg gcgcgcacgc caaaccaccg ctcgatcatg
      781 cggcgagact acccgtgatt tgtcatgggt ccggccaatc cctgacgatc atgtgtcatc
      841 gattcatgcc ctaattactc gcatttgctc acgtcatcgg cgatggttcg cgcgatgtct
      901 gttcagttga ggtcgctacg gggtgagctg acggctgctg gtgtgttccg cacccgcgag
      961 ctgcggagct ggctcgagct agccgcgatg atgagcgtgc tcgcgatgtg cctcatcggc
     1021 atcgcgctcc tcggttggat cggcgcgctg ttcttggttc cggtcgctgc cgtgctgtgc
     1081 acgtcgatct cgatgctcgg ccacgaggga agccatcgca gtttctcggc gtcgccggcg
     1141 cgcaacgcga tcctctgcta tctgacgttc ccgctgttcg gtggtctggg tgcgctgtac
     1201 tggcgcaaca agcacgatcg gctgcaccac ggtcatccga acgtcgaggg catggacccg
     1261 gacattcggc cgtttccgtt ctcgtcgtcg cgcggtgatc acgagcgttg cggccggaag
     1321 acacgttggt tccagcgtca cttccagcgc cggctgttct ggccgatgtc gctgctgatg
     1381 gcgaccggca tgcggcggtc ctcggtcctc tatctcatcc gctatccgaa gaagcgcgag
     1441 gcgggttggt gggcggaggt tctgtgcatg accgcgcact acgccggctg ggtggtcgtc
     1501 ccatcgctga tctggggacc gcttgcgggc atcctcgtct acgtttcgat ctgggggctc
     1561 gtcggcgtgt gtctcgcgct cgtgttcgca cccgcacaca tgggcctgcc gatcgtcgcc
     1621 gaccagaacc atgactggct ccaccagctc gagacgacgc ggaatttgca gctgccgcgc
     1681 gtcatctcgt tcttcttcat cggcctcgac taccaggtcg agcatcactt gttcccgaag
     1741 atcgcgcacg cgaacctgcc gcaagccgct gccatcacgc aggcttggtg caaacgccac
     1801 ggcgtcacgt accagaccat gccctacctc gacgcactcg gtgacgcggt tcggttcatg
     1861 gcggacgcgt ggacgcgcga agcatcgacg gcaagcgcga tgcgcatggc cagctgagtc
     1921 agcgaactca cgatcttcat gatcgttgat accggaccgt agttgccggc agatcattag
     1981 tgtggagctc gcgcgcggca cgatcgccga acgaccctgg gccgtcacgc tcgccatgtt
     2041 cgcgcgccgc tgcgcgagcg ggcagctgac cctgctggcc ggcgataagc gctactcgat
     2101 cgcatacgat cgcggctcga tcgtggcggc gcggtcaccg ctgatcgcag atagcgcggc
     2161 acgcgtcgcg ctgacgagcc acatcgcgac gccgacacac atccgcgagc tcgaacgcca
     2221 gcaggcgttg atcccggggc gcgacgatgt cgatctgctc gccgcgatcg cgcgactgtc
     2281 gccagggcac atcaaccgcc tgcgcgccga cacgatcatt cgcgcggcgg cgcgcacgtt
     2341 cgcgatcgat gcgggtgagt acatcttcga agacactgcg tgtttgcccg ggcgcggtgt
     2401 cgatgtcgat gtgcgcgcga tcatctatca cggcatccac atgcatctgt cggatcagcg
     2461 gctcgctgcc gacctgcgcc gacttggcgg tgcgctgttc gtgctcgaag atcaggcgtc
     2521 ggacgagctg cacaagtttg gcttctgcga gggcgaatgg ccgctgctcg ccgcgctacg
     2581 cgaaggtgcg agcctgccgg agctcgaggc gcgtcatcgg gagatcgatc cacgcacgat
     2641 gtactcgacg gtctatgcgc tcgtcgcgtg cggaacggcc cgcgtcgtcg cgccgagccg
     2701 gctcggcacg ccgccccagt tcttgcgaac gaagtcgaac cccgagcttg cggccgaagc
     2761 ggccgaacgt gccgaacgcg cgctcaagaa cgagcagctc gagacggcgg tgctcgagct
     2821 caagaccgca tgcgagctcg tgccgaacaa tgtcgattac aacgcgctac tcggctgggc
     2881 actgttctgc gcgtcgcacg acaaacacag tgccgccgtc gacgccaaga aggcgctcga
     2941 acgcgcggta catcgctcgg atcgcccgca ggtcgcgcgc ttctacctcg gccgcatcga
     3001 gcgcatgctc ggtcgcgata aggaggcgtt gcgccacttc caggctgtgc tgctcgaaga
     3061 gccgcacaac accgacgcct cggcggaggt tcgcgtgctg caggcacgca tgcgccagcg
     3121 cgctaactga tgcggcaata cggctgcttg cggccggtct tcgtcatcac gacctggtag
     3181 agctggccat cgcgcgaccg cgacgcaccg gcagccgcca gcaagtagaa cttccacatc
     3241 aagtagaact tgcgatcgta gcggtgcgag atctccgagt acgtgcggtc gaagttgtcc
     3301 caccacgcca tcagcgtcgg gtcgtagtcc gggccgatgt tgtgcaggtc ctcgcacacg
     3361 aacaggcctt ccatcgcgcg accgatctgc gcgagtgacg gcgcgaccgc gttcgggaag
     3421 atgtacttct cgatgaacgg cgtgccgtgg cgcaagctgc ggttgttcgc gatcgtgtgg
     3481 acgagcgcga cgccttccgg cttcaggcag cgatcgatcg tctccatcat cgtgcggtga
     3541 ttcttcgggc cgacgtgctc catcatgccg atcgacacga cgcggtcgta cgtgcccttc
     3601 acgtcgcgat aatcggagag ccgcagctcg acgggcaggt gcttccacag ctgattgccg
     3661 agcgagacct ggtccttcga gagcgtcacg ccgagcacgg tgcagccgta cttctcggcc
     3721 gcccagctcg caagaccacc ccagccgcag ccgaggtcga gcaccttcat gcccggctcg
     3781 agaccgagct tcttgcagac gagatcgagc ttgtgctcct gcgcttcctg gagcgtcttc
     3841 gctttgtccc agtacgcgca ggtgtagacc atgcgttggt cgagcatgcg cgtgtagaga
     3901 tcgttgccga tgtcgtaatg cgcctcgacc gacgcacccg agcgcgcctt cgcctgcagg
     3961 ttcagcatca ccgccttcac ggtcagcgcg cgcatgcgcc agctgccggt gatcttctgc
     4021 ttgaggttcg cgcgcatgat cttgtcgatc gtgacgtcga gcgcgtcggt ctcccaccac
     4081 tcctccatgt acgcctcgcc gaagccgacc gacgcgtcgc gcagcacgcg ctcgtagaag
     4141 cgcgggtcgt ggacgcggat gtcgccgggc gcatggccac cgatctcgat gccggcaagc
     4201 ccgaacagct cgcgaacgaa cgattcggcc tcgaccgagc gggtgggaag cgtgatcggg
     4261 atgaaccgct cgatcaatga cttggcgggg ctatgcgttt ccatccggga gggagtcgca
     4321 cggacaccgg ggccgttcaa gcgtttagtc gccctgactg tggcgagcaa gcacagttgc
     4381 tcgcgcccgg gctccggaca ataccgcaga taggtgatca aggacctctg gtacaagaac
     4441 gcgatcgtct actgcgttca cgtcggtgcg ttcatggatt cgaacggcga cggcagcggc
     4501 gacttcgagg gcttgatgcg tcgactcgac tacctcgcgg gtctcggcgt gacgtgcatc
     4561 tggctgatgc cgttccagga atcaccgcag cgcgacgacg gctacgacat cagcgactac
     4621 tactcggtcg atgatcgcta cggctcgctc ggcgacttcg tcgatttcac gcacgaggcg
     4681 aagcagcgcg gcatccgggt gatcatcgat ctcgtcgtca atcacacgtc cgatcagcac
     4741 aagtggtttc aagaggcgcg gcgtgacaag cactcgcgct atcgcgatta ctacatctgg
     4801 tcggagaaga agccgccgga ggccgagcac ggcatggtgt ttcccggtgt gcagaagacg
     4861 acgtggacga aggatgccgt cgccggccag tactacttcc atcgcttcta cgactttcag
     4921 cccgacctca acacgcacaa cccacaggtc caggaggaga ttcgcaagat catgggtttc
     4981 tggctcgagc tcggcgtctc gggctttcgc atggacgcca tcccattcgt gatccagacg
     5041 aaaggcgccg acaagaagca caccgagaag tacgagatgc tgcacgacat gcgcgacttt
     5101 ctgcagtggc gcaccggcga cgcgatcatc ctcgccgaag cgaacgtcac gcccgatcac
     5161 aacctgaact acttcggcaa agacggcgat cggctgcaga tgatgttcaa cttctgggtc
     5221 aaccagcgca tgttctttgc gctcgctggt cacgacgcac ggccgttgcg caaggcgctc
     5281 gagcagacga agaatcgccc cgacgccgcg cagtgggcga tgttcttgcg caatcacgac
     5341 gagctcgacc tcggccggct ctccgacgag gaacgcgaca tcgtgtttcg cgaaatgggc
     5401 gcggagaaga cgatgcagct ctacgaccgc ggcattcgcc gccggcttgc accgatgctc
     5461 aacggtgatc gccggcggat cgagctcgca tactcggtga tgttctcgct gccgggcacg
     5521 ccgatcatgc gctacggcga cgagctcgga atgggcgaca acatgcgact caaggagcgc
     5581 aatgcggtgc gcacgccgat g
//
LOCUS       DASUFX010000673         7346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_525677,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000673 DASUFX010000000
VERSION     DASUFX010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525677"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFV99_27165"
     CDS             <1..347
                     /locus_tag="VFV99_27165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063097.1"
                     /translation="PYRWLALTEIQLGDCPSALPNIEGFVSRVDTTDPRLPEMTRWRE
                     FCSRSQPKPPPQPPAPPPPKSPSLHERWWFWPVVGTAAVALTGGIVYAATRSDEPAML
                     PAIRCDSTGCHR"
     gene            344..1015
                     /locus_tag="VFV99_27170"
     CDS             344..1015
                     /locus_tag="VFV99_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063098.1"
                     /translation="MKRLALLLLASCGDAEPCDGVTGRCIAVHVDSEIVERIDQLELD
                     VLYGDRHGTVTTSDGVVDLPVTTAITLPGTGKLTVGVVAAGKLSGMVLGTGAAETTVA
                     SAAHVELAIKLAKPVACIAGSFYCGGDKVAGDPDVLYQCNGGGVPLARGTCINGCSIM
                     PADNDECAPGDLPCVEPGLYCGGDKVAGDPQTLYRCMSGVGVKVMTCADGCIIEPSPN
                     DDHCR"
     gene            complement(1012..1908)
                     /locus_tag="VFV99_27175"
     CDS             complement(1012..1908)
                     /locus_tag="VFV99_27175"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5063099.1"
                     /translation="MTRLVLVGLFFGACTADVEVLEDLDPAGTNGDEQWSDEGPLEQD
                     VASDVDDVEVTIPEDEITTIAAGRPLFQLPFPCGQVWAGQTRTNHSPQRSVDFNRLND
                     LGDSVVAAAGGTVTRVANEGNVSYGRWIEIAHGAGWRTRYAHLSSQLVHVGQVVHKGQ
                     LIGKVGSTGGSTGPHLHFEERLNGAAVSADFNGRGALYFGTKNYTSQNSCGGGGGNSV
                     VGRVNTAGAPLTKRAGTSTSTAAVGSVADGATVTITCQKRGQSITGTYGTSTLWDHIS
                     GGYIADAYVSTGSDGQVAPTCP"
     gene            2062..2583
                     /locus_tag="VFV99_27180"
     CDS             2062..2583
                     /locus_tag="VFV99_27180"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX5063100.1"
                     /translation="MAVIFIDGNDLRAAEVRTIVPNIDLVVEALQTGFATSAPEPEHK
                     ARDKVLAHKVAAACFAEAVDLTTMDGKSLRLELDSENENRFCKWWRETPAQMQLRIAV
                     RRAGSSDISVVSATCQGRIADKPAGPLLLGWDRLFVPDGEDATLAQLTAAGEVTEFRR
                     AAYADVARTLGLT"
     gene            2583..3500
                     /locus_tag="VFV99_27185"
     CDS             2583..3500
                     /locus_tag="VFV99_27185"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5063101.1"
                     /translation="MGRFDERATQPSRGPSDMFKWKVLRRSDPRPDDFAALDAIRPGI
                     RDGGAQALASHEPVAVWIGHATWALRLGGKLIVTDPVFSKSLSGVVPRLVPPGVALDA
                     LPPVDIVLVTHDHRDHMDLPSLAKLGDAPLYIVPVGNGKRIKHPNVVELDWWQTHDVG
                     DLAITLVPARHWSMREPWNKNDTLWGGFVVRAPEGVAYHSGDTAFGEHFAEIAERVGA
                     IDWAMLPIGGYNPRWFMEPQHIDPIEATRGFAALGARHLLAMHWGTFRLTDEAVGEPP
                     HRLRTYWREQGLPDDRLWILDVGEARSLR"
     gene            3616..5205
                     /locus_tag="VFV99_27190"
     CDS             3616..5205
                     /locus_tag="VFV99_27190"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5063102.1"
                     /translation="MAISGGRSKAFRKLQRLIGIADEADRTGMPSKEAVELVREHERA
                     AFSRRKFLGGAAAAGALALTSRKAFAAPNKPNPDVGIVGAGLAGLQCAYTLSTYGVTA
                     SLYEASSRVGGRQKSLRGFFPGQVAELGGELVDTGHKMMLGWINKLNLTREDLRDPAD
                     DFWYIDGQLVPEATVVDQYRAFVASMRADLQACSGAPTVDGFNAADVALDRTSLHDYL
                     VSRGAGNIARKAIEAAYVGEYGRECTEQSCFNFLLYIHADKRSKFTPFGVFSDERFHV
                     VEGQDRIASGIAAALPRQPEFGLTLVRVAKTSGGRIELTFKKGNTTVVKTHDHAVLAI
                     PFTVLRNVQLDASLQLPAWKTQAINQLGYGDNAKLMVGFKGPFWLDVGCNGTAYANLP
                     NLQGTWETNPSLATTEHAIMTDYTGGVLGKNMSSNVQNETAKFLADLEKIFPGATAKA
                     MKSGNSYKAVLEHWPSNPLALGSYTCYLPGQFTTIAGNESKPIGNLMFAGEHANSFYV
                     WQGFQEGALTSGYDTAIELLA"
     gene            complement(5206..5871)
                     /locus_tag="VFV99_27195"
     CDS             complement(5206..5871)
                     /locus_tag="VFV99_27195"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX5063103.1"
                     /translation="MWRWVLVGLCACRIGFDELPASDASDTVVTTVTFGERPTSQHKN
                     VTRDATVLGRMGNANFGDLEDLSISEYMPPDIEHSLLRFDLSSIAPATRVVAARLTLA
                     RVDYGDEMPGQVDVLLVGESWTEGNGMSGSGVSWNTRDGTIAWTQPGGTTTMLLTQLV
                     PPATTFTIELDPSIVQNWIDVAANNDGVAIRVPNSSVHYHFHARNSAMAARRPELAID
                     LAE"
     gene            complement(5882..6583)
                     /locus_tag="VFV99_27200"
     CDS             complement(5882..6583)
                     /locus_tag="VFV99_27200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063104.1"
                     /translation="MTAAYQRGDVDEASRQGVLAGPAVVEQALHSKARATQLAGIAAA
                     PYVEARPELLPALADVASGADRRTAIPAAQAARQIARELATEDLPDDIGEDDVMTWRA
                     LFDQLAHSESRFIEVRVLALDTVTSLAQTVTPSSIGFDLATALHDRDPAFRVAAVQLV
                     PRPTPAALRTPLADTVKDDADDKVALAAAQALCGDARDPALAALGARGLARVKKLVAG
                     RPPKLVRDAMRCLKR"
     gene            6699..>7346
                     /gene="lexA"
                     /locus_tag="VFV99_27205"
     CDS             6699..>7346
                     /gene="lexA"
                     /locus_tag="VFV99_27205"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5063105.1"
                     /translation="MAEALTQRQREILDFISASIVERGFPPTLREIGEHFNIRSTNGV
                     NDHLKALEKKGHLRREDLKSRAMRPVLPDGSGEIIPMQRRAMGTGDVQIVSESDDMAE
                     IPILGRVAAGQPILAVENATDTVKVDRVLVGNHREVFGLRIVGESMIEDGIFDGDYVF
                     VKKTPSARTGDIVVAMIEGEATVKRYYPEGDRIRFQPANQNMSPIIVRRADFKSVD"
BASE COUNT         1234 a         2448 c         2410 g         1254 t
ORIGIN      
        1 atccctaccg ctggctcgcg ctcaccgaga tccagctcgg cgactgcccc tctgcactgc
       61 cgaacatcga ggggttcgtc tcgcgagtgg acacgaccga tccacgcctg cccgagatga
      121 cgcgctggcg cgagttctgc agccgctcgc agccgaagcc gccgccgcaa ccaccagcac
      181 cgccgccgcc gaaatcgccg tcgctccacg agcgctggtg gttctggccc gtcgtcggta
      241 cagcggcggt cgcgctcacc ggcggcatcg tctatgcagc gacacgcagc gacgaaccag
      301 cgatgttgcc ggccattcgt tgcgatagca ccgggtgtca tcgatgaagc gcctcgcgtt
      361 gctcctgctc gcgagttgcg gcgatgccga accgtgcgac ggcgtcacgg ggcgctgtat
      421 tgcggtccat gtcgattccg agatcgtcga gcgcatcgat caactcgagc tcgatgtgct
      481 ctacggcgat cgccacggca cggtgacgac gagcgacggc gtcgtcgatc ttccagtcac
      541 gactgctatc acgctgcccg gcaccggcaa gctgaccgtc ggcgtcgtcg ctgccggcaa
      601 gctctcgggc atggtgctcg gcaccggcgc ggcggagacg acggtcgcat cggccgcgca
      661 tgtcgagctc gcgatcaagc tcgcgaagcc cgtcgcatgc atcgcgggct cgttttattg
      721 cggcggcgac aaggtcgcgg gcgatcccga cgtgctctac caatgcaacg gcggcggtgt
      781 gccgctcgcg cgtggcacct gcatcaacgg ctgctcgatc atgccggcgg acaacgacga
      841 gtgcgcgccc ggcgacctgc cgtgcgtcga acccggcctc tactgcggcg gcgacaaggt
      901 cgcgggtgat ccgcaaacct tgtatcgctg catgagtggc gtcggcgtca aggtcatgac
      961 ctgcgccgac ggctgcatca tcgagccgtc gccgaacgac gaccattgtc gttagggaca
     1021 ggttggtgcg acctggccgt ccgagcccgt cgacacgtac gcgtccgcga tgtagccgcc
     1081 gctgatgtga tcccacagcg tcgacgtgcc gtacgtgccg gtgatcgact ggccacgctt
     1141 ctgacacgtg atcgtgaccg tcgcgccgtc ggcgacggaa ccgaccgcag ccgtgctcgt
     1201 gctcgtgccc gcacgcttcg tcagcggcgc gcccgcagtg ttcacccgac cgacgacgct
     1261 gttgccgccg ccgccgccac acgagttctg gctcgtgtag ttcttcgtgc cgaagtagag
     1321 cgcgccgcgg ccgttgaagt ctgcgctcac ggcggcgccg ttgagccgct cctcgaagtg
     1381 caggtgcggt cccgtcgagc caccagtgct gccgaccttg ccgatcagct ggcccttgtg
     1441 cacgacctgg ccgacgtgga cgagctgcga cgagagatgc gcgtacctcg tgcgccaacc
     1501 agcgccgtgc gcgatctcga tccagcgccc gtagctcacg ttgccttcgt tcgcgacgcg
     1561 cgtgaccgtg ccgcctgcag ccgcgacgac ggagtcgccg aggtcgttca gacgattgaa
     1621 atccaccgag cgttgcgggc tgtgattcgt gcgcgtttga cctgcccaca cctggccgca
     1681 cgggaacggc agctggaaca gcgggcggcc cgcggcgatc gtcgtgatct cgtcttcggg
     1741 aatcgtcacc tcgacgtcat cgacatcgga tgcaacgtct tgctcgagcg gtccttcatc
     1801 ggaccactgc tcgtcgccat tggtgccagc gggatcgagg tcctcgagca cctccacatc
     1861 agcggtgcag gcaccgaaga acagtccgac gagcaccagg cgagtcattc gagtcgacat
     1921 cattcgcatt tctcctttgc gtcacctcgc tttcagcaag caccgcgcca cgcagtaact
     1981 gcggacaacc actggctacc ggcgcagccg ctgttgcgcg cggtgttaca ggtgcagacg
     2041 agagtctgct aggttcgcgc cgtggctgtg atcttcatcg acggcaacga cctgcgcgct
     2101 gcggaggttc gcacgatcgt ccccaacatc gatctcgtcg tcgaagcgct ccagaccggc
     2161 ttcgcgacca gcgcacccga gcccgaacac aaggcgcgcg acaaggtgct cgcgcataag
     2221 gttgcagcgg cgtgctttgc cgaagccgtc gatctcacca cgatggacgg caagagcttg
     2281 cggctcgagc tcgactccga gaacgagaac cggttctgca agtggtggcg cgaaacgccg
     2341 gcgcagatgc agctccgcat cgcggtgcgc cgcgcgggct cgtccgacat ctcggtcgtg
     2401 tccgcgacct gtcagggccg catcgccgac aaaccggcag gtccgctgct gctcggctgg
     2461 gatcggttgt tcgttcccga cggcgaagac gcgacgctcg cgcagctcac ggccgctggc
     2521 gaggtcacgg agtttcgccg ggccgcgtat gccgatgtcg cgcgtacgct cggactgacg
     2581 taatgggccg cttcgacgag cgcgcgacgc agccatcgcg cggcccttcc gacatgttca
     2641 agtggaaggt gctgcgccgt agcgatccgc gacccgacga cttcgccgcg ctcgatgcga
     2701 ttcgtcccgg cattcgcgac ggcggcgcac aggcgctcgc gagccacgag ccagtcgccg
     2761 tatggatcgg ccacgcgacg tgggcgctgc gtctcggcgg caagttgatc gtcaccgatc
     2821 cggtgttctc gaagtcgctg tccggtgtcg tgcctcggct cgtgccccct ggtgtcgcgc
     2881 tcgacgcgtt accgcccgtc gacatcgtgc tcgtcacgca cgatcaccgc gaccacatgg
     2941 atctaccgtc gctcgccaag ctcggcgacg cgccgctcta catcgtgccg gttggtaacg
     3001 gcaaacggat caagcatccg aacgtcgtcg agcttgactg gtggcagacg cacgacgtcg
     3061 gcgacctcgc gatcacgctc gtgcctgcac gtcactggtc gatgcgcgag ccgtggaaca
     3121 agaacgatac gctgtggggc ggctttgtcg tgcgcgcacc cgaaggcgtc gcgtatcact
     3181 cgggcgacac cgcgtttggc gagcacttcg ccgagatcgc cgagcgcgtc ggtgcgatcg
     3241 actgggcgat gctgccgatc ggcggctaca acccgcgctg gttcatggag ccgcagcaca
     3301 tcgatccgat cgaagctacg cgtggctttg ctgcgctcgg cgcgcgccac ttgctcgcga
     3361 tgcactgggg aacatttcgg ctgaccgatg aagcggtggg cgagccaccc caccgccttc
     3421 gcacctactg gcgcgagcaa ggcttgcccg acgatcggct gtggattctc gatgtaggcg
     3481 aagcgcgaag tttgcgctga ctaccgcagc ttccggttgc gccaactcga cccgccagag
     3541 aaccccaaaa aatacccggc tgcggtgatc gagctgacag tccttacaag catgcttcgt
     3601 gcagagtggc ggaccatggc tatttcaggt ggtcgtagta aggcgttccg caagcttcaa
     3661 cgtctgatcg gcatcgcaga cgaggctgat cgaacgggca tgccgtcgaa ggaagccgtc
     3721 gagctcgtgc gggagcacga gcgggctgca ttctctcgcc gtaagttcct cggcggtgcg
     3781 gccgcggccg gtgcactcgc actgacgtcg cggaaggcat tcgcggctcc gaacaagccg
     3841 aacccggatg tcggcatcgt cggcgccggc ctcgccggtc tgcagtgcgc gtacacgctg
     3901 agcacctacg gcgtgaccgc gtcgctctac gaagcgtcgt cgcgcgtcgg cggccgccag
     3961 aagtcgctgc gcggcttctt ccccgggcaa gtcgccgagc tcggcggcga gctggtcgac
     4021 accggccaca agatgatgct cggctggatc aacaagctca atctgacgcg tgaagaccta
     4081 cgcgaccccg cggatgactt ctggtacatc gacggccagc tcgttcctga agcgacggtc
     4141 gtcgaccagt accgcgcgtt cgtcgcgtcg atgcgcgccg acctgcaagc gtgctccggc
     4201 gcgcctaccg tggatgggtt caatgcggct gatgtcgcgc tcgatcgcac gagcctgcac
     4261 gactatctcg tctcgcgcgg cgccggcaac atcgcgcgca aggcgatcga agccgcgtac
     4321 gtcggcgagt acggccgcga gtgcaccgag cagagctgct tcaacttctt gctctacatc
     4381 catgccgaca agcgctcgaa gttcacgccg tttggtgtgt tctccgacga gcgcttccac
     4441 gtcgtcgagg gccaggaccg catcgcgagt ggcattgctg ccgcgctgcc gcgccagccc
     4501 gaattcgggc tcacgctcgt tcgcgtcgcg aagacgtcgg gcggccgcat cgagctgacg
     4561 ttcaagaagg gcaacacgac ggtcgtcaag acgcacgacc acgcggtgct cgcgattccg
     4621 ttcaccgtgt tgcgcaacgt ccagctcgac gcgagcttgc agctgccggc gtggaagacg
     4681 caggcgatca accagctcgg ctacggcgac aacgccaagc tgatggtcgg cttcaaggga
     4741 ccgttctggc tcgacgtcgg ttgcaacggc accgcctacg ccaacctgcc gaacctgcaa
     4801 ggtacgtggg agacgaaccc gtcgctcgcg acgaccgagc acgcgatcat gaccgactac
     4861 accggcggtg tgctcggcaa gaacatgtcg tcgaacgtgc agaacgagac cgcgaagttc
     4921 ctcgccgacc tggagaagat cttcccgggt gcgaccgcga aggcgatgaa gagcggcaac
     4981 agctacaagg ccgtgctcga gcattggccg tcgaacccgc tcgcgctcgg cagctacact
     5041 tgttacctgc ctggtcagtt cacgacgatc gcgggcaacg agagcaagcc gatcggaaat
     5101 ctgatgttcg ccggcgagca cgcgaactcg ttctacgtgt ggcagggctt ccaggaaggc
     5161 gcgctgacga gcggctacga caccgcgatc gagctgctcg cgtagttact ccgcgagatc
     5221 gatcgcgagc tcggggcgtc gcgctgccat cgcggagttt cgcgcgtgga agtggtagtg
     5281 cacgctcgag ttgggcactc tgatcgcgac gccgtcgttg ttcgccgcga catcgatcca
     5341 gttctggacg atcgagggat cgagctcgat cgtgaacgtc gttgccggcg gcacgagctg
     5401 cgtcagcaac atcgtcgtcg tgcctcccgg ttgggtccac gcgatcgtgc cgtcgcgcgt
     5461 gttccacgac acgccggagc ctgacatccc gttgccttcg gtccagctct cgccgacgag
     5521 cagcacgtcg acctggcccg gcatctcgtc gccataatcg acgcgtgcga gcgtgagccg
     5581 cgccgcgacg acgcgcgtcg ctggtgcgat cgacgatagg tcgaagcgca gcaaactgtg
     5641 ctcgatgtcg ggcggcatgt actccgagat cgagaggtcc tcgaggtcgc cgaagttcgc
     5701 gttgcccatc cgcccgagca ccgtcgcgtc gcgcgtgacg ttcttgtgct gcgacgtcgg
     5761 tcgctcgcca aacgtgaccg tcgtgaccac ggtgtcggaa gcgtcgctcg cgggaagctc
     5821 gtcaaagccg atgcgacacg cgcacagccc gacgagtacc caccgccaca cgcggtcgat
     5881 gttaccgctt caagcagcgc atcgcatcgc ggacgagctt cggcggtctg ccggcgacga
     5941 gtttcttcac gcgcgcgagc ccgcgggcac ccagcgcggc cagcgccgga tcgcgagcgt
     6001 cgccgcagag tgcctgcgcc gcggcgagtg ccaccttgtc gtcggcgtcg tctttgacgg
     6061 tgtccgcgag cggcgtgcgc aacgcagcgg gtgtcggtcg cggcaccagc tgcaccgctg
     6121 cgacgcgaaa cgccggatcg cgatcgtgga gcgcagtggc gagatcgaag ccgatgctcg
     6181 acggcgtgac ggtctgtgcg agcgacgtga cggtgtcgag cgcgagcacg cggacttcga
     6241 tgaagcgcga ctcgctgtgc gcgagctgat cgaacagcgc gcgccacgtc atcacgtcgt
     6301 cctcgccgat gtcgtcgggc aggtcctcgg tcgcgagctc gcgcgcgatc tgacgtgcgg
     6361 cctgcgcggc tggaatcgcc gtgcgccggt cggcgccgct cgcgacgtcg gcgagtgctg
     6421 gcaggagctc tggccgcgcc tcgacgtacg gcgcagcggc aatgccggcg agctgggtcg
     6481 cgcgcgcctt ggagtgcagc gcctgctcga cgacggcagg cccggcgagc accccttgcc
     6541 ggctcgcttc gtcgacgtcg ccgcgctggt aggcggcggt catcgtcgcg atccccgcga
     6601 ccgcaacacc gaaaaatcct agcgaaaaaa ttgacatggt gattgtgcgt tcagtaccgt
     6661 caatgcgttc agtaccacgt cagattcacc ggggtgccat ggccgaagcg ctgactcaaa
     6721 gacaacggga aatcctcgat tttatttccg cttcgatcgt ggagcgcggc tttccgccga
     6781 cgctacgaga gatcggagag cacttcaaca ttcgctcgac gaacggcgtc aacgaccatc
     6841 tcaaggcgct cgagaagaag ggccaccttc gtcgcgagga cctgaagtcg cgcgcgatgc
     6901 gtcccgtgct gcccgacggc agcggcgaga tcatccccat gcagcggcgg gcgatgggta
     6961 cgggcgacgt gcagatcgtg tcggagagcg acgacatggc cgagattccg attctcggtc
     7021 gggtcgccgc gggtcagccg atcctcgcgg tcgaaaacgc gaccgacacg gtcaaggtcg
     7081 atcgcgtgct ggtcggcaac caccgcgagg tgtttggact ccggatcgtg ggcgagtcga
     7141 tgatcgagga tggcatcttc gatggcgact acgtcttcgt gaagaagacg ccctccgcgc
     7201 gtaccggtga catcgtagtg gcgatgatcg agggtgaggc caccgtgaag cgctactacc
     7261 cggaaggtga ccgaattcgg ttccagccgg cgaaccagaa catgagcccg atcatcgtcc
     7321 ggcgtgccga cttcaagtcc gtcgac
//
LOCUS       DASUFX010000674         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1358565, whole genome shotgun sequence.
ACCESSION   DASUFX010000674 DASUFX010000000
VERSION     DASUFX010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358565"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFV99_27210"
     CDS             <1..587
                     /locus_tag="VFV99_27210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063106.1"
                     /translation="GGGGGGGGGGGGSGGGGDGSGSGSGSGSGGGGSTDTSYVSTFLH
                     GLGVKYCDEAFTCKASFPTDAGVTFEQEFGTTAMACYMDSDAYYDAAGVAAAITGGTI
                     TWNPSDAMACLAGITFSTCADFWMNGGTEPAACDTALVGHIADGGACTVTFECSNLQS
                     YCDETTKKCTVDTGGMRTVPTPSVALPTPEAILR"
     gene            complement(926..1666)
                     /locus_tag="VFV99_27215"
     CDS             complement(926..1666)
                     /locus_tag="VFV99_27215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX5063107.1"
                     /translation="MISVRTLSKRHGDRLILDKVSAEVTKGETIAIVGPSGGGKSTLL
                     RCLNYLESFDGGEVTIAGVKLVPGMGTRQRELLRALRVKVGMVFQQFNLFPHLSALDN
                     ITLAPRVVRKLDASAAEKRGHELLTRVGLGDRAGAFPHELSGGQQQRVAIARALAQDP
                     EVLLFDEPTSALDPEMRDEVMSVIKDLAAGGMTMLVVTHEMQFAHDIATRVWVIDRGT
                     IAEDGPPSRVVDNPQSSVAREFFGRLKR"
     gene            complement(1641..>2399)
                     /locus_tag="VFV99_27220"
     CDS             complement(1641..>2399)
                     /locus_tag="VFV99_27220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5063108.1"
                     /translation="RKDGELEQILRKAKLWDQRQTEPPPATAETAAKKRQFDGEMFWQ
                     FVDAALVTLKLSLLAFFLAVPIGLLLAVARVYGNAVTSALARIYIEVFRGTPVLLQLF
                     VLYYGLAPYYSLGPVQAAVLGLGLNYGAYEAEVYRGALLAIPRGQTEAAKALGMSPIL
                     TLRHVLLPQALRLALPPMTNDFVSLLKDSSLVSVITVIELTKRMTIAAVDMRGWLIPG
                     LACAGLYLALSFPLSELARRLERRLARDQRPHAL"
BASE COUNT          405 a          773 c          825 g          396 t
ORIGIN      
        1 gcggtggcgg tggtggcggt ggcggcggag gaggaggcag tggtggcggt ggcgatggca
       61 gcggcagcgg cagcggtagt ggctccggcg gcggtggcag caccgatacg agctacgtat
      121 cgacgttcct ccacggcctc ggcgtgaagt actgcgacga agcattcacg tgcaaagcga
      181 gcttcccgac cgacgcaggc gtcacgttcg agcaagagtt cggaacgacc gcgatggcgt
      241 gctacatgga ctccgacgcg tactacgacg ctgcgggcgt cgcggcggcg atcaccggcg
      301 gcacgatcac gtggaatccg agcgacgcga tggcgtgcct cgccggcatc acgttcagca
      361 cgtgcgctga cttctggatg aacggcggca ccgagcctgc ggcatgtgac acggcgctcg
      421 tcggtcacat cgccgacggt ggagcgtgca ctgtcacgtt cgaatgctcg aacctacagt
      481 cgtactgcga cgagacgacg aagaagtgca ccgtcgacac cggcggcatg cgtacggtac
      541 cgacgccgtc ggtcgcgctc cctacgccgg aggcgatcct ccggtagcgc gcgtccgtcg
      601 agcgaaccct cgtatcggtt tttgacggcg gtaccgatgc cggatgcgcc gcagcggcaa
      661 ctgcgggtga cgcaggtgtc aaccgcggat gactgtggcc cccaccgcgt gaacggtggg
      721 gaccggatac atgggcggca cgtgtgtttt ttgcgcatcc accattcacg tgccgcccga
      781 acaagccgtc tccagcttgt ccattcttgt cgaacgcttg gtccctagtg cagcgcatgt
      841 gccacgcgca acgtcgcggc tgcattgcga gcacgagcgc gagcgtgatc ctcgcgggtt
      901 acgaagcgca gatcatgggt gacggctagc gtttcagccg gccgaaaaac tcccgcgcga
      961 ccgagctctg cggattgtcg acgacccgcg atggtggccc gtcttcggcg atcgtgccgc
     1021 gatcgatcac ccagacgcgc gtcgcgatgt cgtgcgcgaa ctgcatctcg tgggtgacga
     1081 cgagcatcgt catgccgccc gccgcgagat ctttgatcac gctcatcacc tcgtcgcgca
     1141 tttcgggatc gagtgcactc gtcggctcgt cgaacaggag gacctcggga tcttgtgcga
     1201 gagcgcgcgc gatcgcgacg cgttgctgct gccctccgga tagctcgtgc ggaaacgctc
     1261 ccgcgcgatc gccgagaccg acgcgcgtga gcagctcgtg cccgcgcttc tcggctgcgc
     1321 ttgcatcgag cttgcgcacg acgcgaggcg cgagcgtgat gttgtcgagc gcggacaggt
     1381 gcgggaacag gttgaactgc tggaacacca tgccgacctt cacgcgcagt gcgcggagca
     1441 gctcgcgctg gcgcgtcccc atgcccggca cgagcttgac gcccgcgatc gtgacttcgc
     1501 cgccgtcgaa gctctcgagg taattgaggc accggagcag cgtcgacttg ccgccacccg
     1561 acggcccgac gatcgcgatc gtctcgccct tcgtgacctc ggcgctgacc ttgtcgagga
     1621 tcagccggtc gccgtgacgc ttagagagcg tgcggacgct gatcacgcgc cagcctcctc
     1681 tcgagtcgtc gcgccagctc ggatagcggg aagctgagtg cgaggtagag gcccgcgcac
     1741 gcaaggccgg gaatcagcca gccgcgcatg tcgacggcag cgatcgtcat ccgcttcgtc
     1801 agctcgatga ccgtgatcac gctgacgagc gatgaatcct tgagcagcga gacgaagtca
     1861 ttcgtcatcg gcggcaacgc gagccgtagc gcttgcggca acagcacgtg gcgcagcgtc
     1921 aggatcgggc tcatgccgag cgccttcgcc gcttcggtct ggccgcgtgg aatcgcgagc
     1981 agcgcgccgc gatagacctc ggcttcgtac gcgccgtagt tgaggccgag accgagcacc
     2041 gccgcttgga ccggaccgag gctgtagtac ggcgcgaggc cgtagtagag gacgaacagc
     2101 tgcagcagca ccggggtgcc gcggaacacc tcgatgtaga tgcgcgcgag cgcgctcgtc
     2161 acggcgttgc cgtagacgcg cgcaaccgcg agtagaaggc cgatcggcac cgcgaggaag
     2221 aacgcgagta gcgacagctt cagcgtgacg agggccgcat cgacgaactg ccagaacatc
     2281 tcgccgtcga actggcgctt cttcgcggcc gtctcggcgg tcgccggcgg cggctcggtc
     2341 tggcgctggt cccacagctt tgccttgcgc aggatctgtt cgagctcgcc gtccttgcg
//
LOCUS       DASUFX010000675         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_107451,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000675 DASUFX010000000
VERSION     DASUFX010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107451"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFV99_27225"
     CDS             complement(<1..230)
                     /locus_tag="VFV99_27225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063109.1"
                     /translation="MRSRRAWIYVLSVLALLVPAGGIAYLGAFSFRDERGAVTAQTER
                     QDQTADAIARRVSIAIDAAFDAIERAVAAKQTP"
     gene            371..1075
                     /locus_tag="VFV99_27230"
     CDS             371..1075
                     /locus_tag="VFV99_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX5063110.1"
                     /translation="MAKALIIVPTYNEAENVRGIAEGLLEALPPADVLFVDDNSPDGT
                     GALIDEMAASNPRVHVLHRAGKLGLGTAYIEGFGWGLSREYEYLFEMDADGSHDPKYL
                     PQMLALAEDGADVVVGSRNIPGGGTVNWGIGRKILSKGGSFYARTILGIDVRDVTAGF
                     ICWRRHVLESLDLTGIDSNGYSFQIEMKYRAAQKGFRIVETPIVFVDRRVGQSKMSRA
                     IVAEALLKVWALRFRR"
     gene            1168..1788
                     /locus_tag="VFV99_27235"
     CDS             1168..1788
                     /locus_tag="VFV99_27235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5063111.1"
                     /translation="MNILRLRIKSADEWRELAPSENVTEPIFVPTTDELKSGEQVIVE
                     ISSNLLPNKVLIRGSVQSWRPALPRMRVRAGATVQLDVDEQVKLTFLHDVLSGKRTDV
                     PRRRHHRLPVQFACRYRVANSPSFVESAISEIGVGGALLTTSSPLPLDTELTLEVTPP
                     GAAAPIAIAGRVSYHAPSGGSGLRFVSRDGDGDRRLRELIRRLRAS"
     gene            1785..2300
                     /locus_tag="VFV99_27240"
     CDS             1785..2300
                     /locus_tag="VFV99_27240"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5063112.1"
                     /translation="MTDKKRPPRRFVPRALEMRSAADAVKAALAFHGISDRVRAERVL
                     TEWFELVGQKIASRTRPYGVTDRVLVIEVASSAWLHELNLLKAQILAGLLDRLGEPKL
                     FDELKFRLAGSSGGRPSLIRPKPRPAAKPQPAPTPATGAAREQIAREVEKIDDDELRE
                     LIARVRIQNDR"
     gene            complement(2308..3936)
                     /locus_tag="VFV99_27245"
     CDS             complement(2308..3936)
                     /locus_tag="VFV99_27245"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5063113.1"
                     /translation="MKGLSAGVRVGILFILLAVGAYVVWKNLGSTEEKSGQQFFVRMR
                     DASNLPRGSKVVVAGLKKGQVTKLEVDGRYAKIYFNLSEAIPVWSSAIVYKKASSLLG
                     ENYLEIDPGEEIRQAPDGTTVKYTRLGPLCPDYDNRTNQAKQDACREIKMRVEAVTPD
                     QLIARIEASTQNVDKVLESVRDLSEDVRHIVNGPMARVANRIDGLVQKEADTVQSIIE
                     RADRTLAAVEGLTKDVRNITSSADPKIAKMLDDLQAASAEAKVLVQTAKQELQQTGDS
                     VRGKLDKLDGVIDSTNSIAKKIDEDKGTLGRLVNDPAIADNVESITDDAKGFLGTLFG
                     LKAYVGLRSEYNFESQLLRSYVSVELHTRPDKFYLIEVVKEPRGNYPEVSLTFDPTID
                     ANNWIKKTVIKDEFRFTFQFAKRFNWLTLRYGIKESTGGVGADADGRWYDKNLRMSLD
                     VFDATWDNLPRVRLTAAMELFRHLYILGGIDDALNQPQQLTIVTGTSQVPVEFEEYHY
                     GRDYFLGGMLRFNDEDLAALLTVGGSAIAGAGKN"
     gene            complement(3933..4763)
                     /locus_tag="VFV99_27250"
     CDS             complement(3933..4763)
                     /locus_tag="VFV99_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5063114.1"
                     /translation="MSDAALASEIIGLSPPDQADPRWHIRVRQLTKSFGTNLVLDKVD
                     LDLERHKVNVILGASGAGKSVLIKHFMCLLRPDSGHLWVDGTDVFSLNTKELGTFRRK
                     FGLVFQFAALFDSLTVEENCAFPLREHTNKSKSEIRDIVADRLGSLGLAGTQRKYPGE
                     LSGGMRKRVGLARALVLEPEILMYDEPTTGLDPLATRNVDEMILEAGERYKVTSVVIS
                     HDMASVFRIADRIAILHNKRILEAGPAEVIAASRDPYVYEFLRASGVSAIGKLAGGHA
                     "
     gene            complement(4760..>5210)
                     /locus_tag="VFV99_27255"
     CDS             complement(4760..>5210)
                     /locus_tag="VFV99_27255"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5063115.1"
                     /translation="ELGTMRITEQIDALESMAVNPHQYLILPRLSAGIIVTPILALIF
                     FVVAMGGAYIVAVLVEGVPQGPWIANLREIVEVRDVAQGCIKAIFFGFMIAMIGCYQG
                     YHASGGGRGVGIGTARAVVIASVATLVMDYFLTDILFHLMPSSRGEW"
BASE COUNT          900 a         1720 c         1653 g          937 t
ORIGIN      
        1 ggcgtctgct tcgcggcgac tgcgcgctcg atcgcgtcga atgccgcgtc gatcgctatc
       61 gagacccggc gcgcgatcgc gtccgcagtc tgatcctggc gctcggtctg ggcggtcacc
      121 gcgccgcgtt catcgcgaaa cgagaacgcg ccgagatagg cgatgccgcc ggccggcacg
      181 agcagcgcca gcacggacaa gacatagatc caggcgcgcc gggatctcac cccagtcagc
      241 atagcgcggg acccccaatg cgccgaaatc gggttcggtc ctggcattcc acggggacac
      301 accgcgccgc gcgcgatatc cccggccgcc gctttccgat ccggccctcc cccgctatcg
      361 tccgccgccg atggccaaag cgctcatcat cgtgcctacg tacaacgagg ccgaaaacgt
      421 gcgcggcatc gccgagggcc tgctcgaggc gctccccccg gcggatgtcc tgtttgtcga
      481 cgacaactca ccggacggca ccggggccct gatcgacgag atggctgcca gcaatccacg
      541 cgtgcacgtc ctgcatcgcg ccggcaagct cggcctcggc accgcctaca tcgagggatt
      601 cggctggggc ctgtcccgcg agtacgaata cctcttcgaa atggatgccg acggcagcca
      661 tgatccgaag tacctgcctc agatgctcgc cctcgccgag gacggcgccg acgtcgtggt
      721 cggctcgcgc aatatcccgg gcggcggcac ggtcaactgg ggcatcggcc gcaagatcct
      781 ctccaagggc ggcagcttct acgcgcgcac gatcctcgga atcgacgtcc gcgacgtcac
      841 cgccggcttc atttgctggc ggaggcacgt gctggagtca ttagacctca ccgggatcga
      901 cagcaacggt tacagcttcc agatcgagat gaagtaccgc gccgcgcaga agggcttccg
      961 catcgtcgag acccccatcg ttttcgtcga tcggcgggtc ggccagtcga agatgtcgcg
     1021 cgccatcgtc gcagaggccc tgctgaaggt ctgggcattg cgttttcggc gctaatcggc
     1081 gctatcgcct agaaaaatgc cgatcgcgtt gcgccctgtc gagtggcgca actaccccat
     1141 cctttgctac ggtcgtcgag gctcgagatg aacatcctgc ggcttcgcat caagtcggcc
     1201 gatgaatggc gcgagctcgc tcccagcgag aacgtgaccg agccgatctt cgttcccacg
     1261 accgacgagc tgaagtctgg cgagcaggtc atcgtcgaga tctcatcgaa tctcctcccc
     1321 aacaaagtgc tgatccgcgg ctcggtgcag tcgtggcgtc ccgcgttgcc acgcatgcgc
     1381 gtgcgcgccg gtgccaccgt gcagctcgat gtcgacgagc aggtgaagct gacgttcttg
     1441 cacgacgtgc tgtccggcaa acggaccgac gtgccgcgtc gccgtcatca ccgcttgccg
     1501 gtgcagttcg cgtgtcgcta tcgcgttgcg aactcgccga gcttcgtcga gtcggcgatc
     1561 tccgagatcg gcgtcggtgg cgcgttgctc accacgagct caccgttgcc gctcgacacc
     1621 gagctcacgc tcgaagtcac gcctcctggt gcggccgcgc cgatcgcgat cgcgggtcgc
     1681 gtctcgtatc acgcgccgtc gggtggctcg ggcctgcgct tcgtcagccg cgatggcgat
     1741 ggcgatcgcc gcctgcgcga gctgattcgc cgcctccgcg cgtcgtgacc gacaagaagc
     1801 gaccgccgcg tcgcttcgtg ccgcgcgcac tcgagatgcg cagcgccgcg gatgcagtca
     1861 aagctgcgct cgcgtttcac ggcatcagcg atcgcgtgcg tgccgagcgc gtgctcacgg
     1921 agtggttcga gctcgtcggt cagaagatcg cgagccgcac gcggccctat ggcgtgaccg
     1981 atcgcgtgct cgtgatcgag gtcgcgagct cggcgtggct ccacgagctc aacttgctca
     2041 aggcgcagat cctcgccggc ctgctcgacc gcctcggcga gccgaagctg ttcgacgagc
     2101 tcaagttcag gctcgccggc agcagcggcg gacggccgtc actgatccga ccgaagccgc
     2161 ggccggccgc caagccgcag cccgcgccga caccggcgac cggcgcagca cgtgaacaga
     2221 tcgcccgcga agtcgagaag atcgacgacg acgagctgcg cgagctgatc gcgcgcgtac
     2281 gaatccagaa cgaccggtag ctcgcggcta gttcttgcca gcgccggcga tcgccgagcc
     2341 accaaccgtg agtagcgcgg cgaggtcttc gtcgttgaag cgcagcatgc cgccgaggaa
     2401 gtaatcgcgg ccgtagtgat actcctcgaa ctcgacgggc acctgcgagg tgccggtgac
     2461 gatcgtcagc tgttgcggtt ggttcagcgc gtcgtcgata ccgccgagga tgtagaggtg
     2521 gcggaacagc tccatcgcgg ccgtgagcct cacgcgcggt aggttgtccc aggtcgcgtc
     2581 gaacacgtcg agcgacatgc ggaggttctt gtcgtaccag cgcccgtcgg catcggcacc
     2641 gacgccaccc gtcgattcct tgatgccgta acgcagcgtc agccagttga agcgcttcgc
     2701 gaactggaac gtgaaccgga actcgtcctt gatcacggtc ttcttgatcc agttgttcgc
     2761 atcgatcgtc ggatcgaacg tcaggctgac ctcgggatag ttgccgcgcg gctccttgac
     2821 gacctcgatc aagtagaact tgtcgggacg cgtgtgcagc tcgacgctca cgtacgagcg
     2881 caacagctgc gactcgaagt tgtactcgct gcgcagaccg acgtacgcct tgaggccgaa
     2941 cagcgtaccg aggaagccct tcgcgtcgtc ggtgatcgac tcgacgttgt ccgcgatcgc
     3001 cggatcgttg acgaggcggc cgagcgtgcc cttgtcttca tcgatcttct tcgcgatcga
     3061 attcgtcgaa tcgatcacgc cgtcgagctt gtcgagcttg ccgcgcaccg aatcgccggt
     3121 ctgctgcagc tcctgcttcg cggtctgcac gagcaccttc gcctcggccg atgcggcctg
     3181 cagatcgtcg agcatcttcg cgatcttcgg atccgcgctc gacgtgatgt tgcgaacgtc
     3241 cttggtcagg ccctcgacgg cggcgagcgt gcgatcggcg cgctcgatga tcgactgcac
     3301 ggtgtcggct tccttctgca cgaggccgtc gatccggttc gcgacgcgcg ccatcggacc
     3361 gttgacgatg tggcgcacgt cttcggatag atcgcgcacg ctctcgagca ccttgtcgac
     3421 gttctgcgtc gatgcctcga tgcgcgcgat cagctgatca ggcgtgaccg cctcgacgcg
     3481 catcttgatc tcgcggcagg cgtcctgctt cgcttggttc gtgcggttgt cgtagtccgg
     3541 gcacagcggg ccgagtcgcg tgtacttcac agtcgtgccg tccggcgcct ggcgaatctc
     3601 ttcgcccgga tcgatctcga ggtagttctc gccgagcagc gagctcgcct tcttgtagac
     3661 gatcgcgctc gaccacaccg gaatcgcttc gctgaggttg aagtagatct tcgcgtagcg
     3721 gccatcgacc tcgagcttgg tgacctgccc cttcttgaga ccggcgacga ccaccttgct
     3781 gccgcgcggc aggttcgacg cgtcgcgcat gcgcacgaag aactgctggc cgctcttctc
     3841 ttccgtgctg cccaggttct tccacacgac gtaggcgcct acggcgagca ggatgaacag
     3901 gatgccgact cgtacgccgg cggagaggcc tttcatgcgt ggcccccggc gagcttacca
     3961 atcgccgaga cgcctgacgc acgcaggaac tcgtagacgt acgggtcgcg gctcgccgcg
     4021 atcacctcgg ccggaccagc ctcgaggatc cgcttgttgt gcagaatggc gatgcggtcc
     4081 gcgatccgga ataccgacgc catgtcgtgc gaaatgacca ccgacgtaac tttatatcgt
     4141 tcgccggctt cgagaatcat ttcgtcgacg ttgcgggtcg caagcgggtc caaacccgtc
     4201 gtcggctcgt catacataag tatttcaggc tcgagcacga gtgcgcgtgc cagaccaacg
     4261 cgcttgcgca taccacccga gagctcacct ggatacttgc gctgcgtacc tgcgagcccg
     4321 agcgagccga gccgatcggc gacgatgtcg cggatctcac tttttgattt gttggtgtgc
     4381 tcacgaagtg gaaaggcaca gttctcctcg acggtgagcg aatcgaacag cgccgcgaac
     4441 tgaaacacga gaccgaactt gcgacgaaac gtgccgagct ctttcgtgtt gaggctaaac
     4501 acgtcggtgc cgtcgaccca gagatgacca ctgtcgggcc gcaagaggca catgaaatgt
     4561 ttgatcaaga cactcttgcc ggcgccggac gcgccgagga tcacgttgac cttgtgccgc
     4621 tcgagatcga gatcgacctt gtcgagcacg aggttcgtgc cgaacgactt cgtgagctga
     4681 cggacgcgga tgtgccagcg cgggtcggcc tgatcgggcg gcgacaggcc gatgatctcg
     4741 gacgcgagcg cggcgtcgct caccactcac ctcgcgatga cggcatgagg tggaacagga
     4801 tgtcggtcag gaagtagtcc atgacgagcg tcgcgaccga cgcgatcacg accgcgcgcg
     4861 cggtgccaat gccaacgccg cgaccaccac cgctcgcgtg atagccctgg tagcaaccga
     4921 tcatcgcgat catgaagccg aagaagatgg ccttgatgca accctgcgcg acgtcgcgga
     4981 cttcgacgat ctcgcgcagg ttcgcgatcc acggaccttg cggaacgccc tcgacgagca
     5041 ccgccacgat gtacgcgccg cccatcgcaa cgacgaagaa gatgagcgcg aggatcggcg
     5101 tgacgatgat gccggcgctg aggcgcggca ggatcagata ttgatgcggg ttgacggcca
     5161 tcgactcgag cgcgtcgatc tgctcggtga tgcgcatcgt gccgagctcg
//
LOCUS       DASUFX010000676        11602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_107643,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000676 DASUFX010000000
VERSION     DASUFX010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107643"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="era"
                     /locus_tag="VFV99_27260"
     CDS             <1..459
                     /gene="era"
                     /locus_tag="VFV99_27260"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5063116.1"
                     /translation="VEVVPISAATGDNVDALERVIAQRLPAGPPLFPQGMVTDRSPQF
                     IAQEIIREQLYHQLGKELPYACAVQVETWTERKGELVIGGVIVVERESQKPIVVGKAG
                     SRIKELGIAARTALAAALGKPVHLSLFVKVREDWSQTEKDLRELGYGGDK"
     gene            456..1886
                     /gene="der"
                     /locus_tag="VFV99_27265"
     CDS             456..1886
                     /gene="der"
                     /locus_tag="VFV99_27265"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5063117.1"
                     /translation="MIAPLLAVIGRPNVGKSTLFNRLVGGRPALVHDTPHLTRDRRYG
                     ETDYFGKELRLVDTGGLDPEAATEVIGAGIHRQAMRAIDEADALLLVVDGRAGVSPID
                     HKLAKELRATSKPVFLAVNKIDHPTTDHLVHEFYELGLGNPFAISAAHGRGIDALLEA
                     ITDAVVKTPVADVGPPDDEEDDLYDVLAEGDEAAEETDEMAAKRAARDAEPLRVAFVG
                     RPNAGKSSLTNRLLGEERSLVHDVAGTTTDPVDAKFTIGGREYVLVDTAGIRRKARID
                     EEIEKISVSMAIGQIERADVAVLVIDAGIGPSEQDARIAGKIEEAGRALVIALNKSDL
                     VQGEEKKSALRDLTKDTFHFLDWAPVVNLSAQRGDGVDRLLDVVDQVAKEHKKRIPTA
                     QLNRFFADVIEEMPPPLHKGYAVRIHYITQGATRPPTFILMANKPELAPSYKRFIANR
                     LRKAYGFKGTPIRIFVKARKDRHDTD"
     gene            complement(1887..2213)
                     /locus_tag="VFV99_27270"
     CDS             complement(1887..2213)
                     /locus_tag="VFV99_27270"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5063118.1"
                     /translation="MALVAMVLVAAAPPPTPKLLETGKQVYDVNCAACHGATGEGNGP
                     VAFAVKPPPRNLVKDPFKAGDGVEQIFKTVSTGLPNTKMVGYPTIKEADRWALAYYVR
                     AFRIKK"
     gene            complement(2228..2797)
                     /locus_tag="VFV99_27275"
     CDS             complement(2228..2797)
                     /locus_tag="VFV99_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063119.1"
                     /translation="MALTLRPTQLLPVIVCALLAPYGEARAEGERALSVGLSWATFSV
                     PGVAEGNMEPPNVTPTVGGALTGIYEQSISTDFSLRGELAGGLFYGGQKMDQAALSYA
                     GLIDVGAVLRFDVLKYVPYAFGGIGGMFTTGGPIGDNASLVLAVGGGLDVLTSRERSW
                     GVEARLASFGGDVTMFTFGLRGTIRWGYF"
     gene            2832..3596
                     /locus_tag="VFV99_27280"
     CDS             2832..3596
                     /locus_tag="VFV99_27280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063120.1"
                     /translation="MADKDNKNPTKPAGTSAKPGFDPKPVQVGGESLVERLIPHMKQI
                     VIGILVIAVALTVVFTVRYFKERGRQKDTAKLEHVLDVASRQVRPPGVEPDPKAKEPT
                     FANNKERAIAVLAAAQKEGSKATEGAFKASMFVQADQLDDAIAEYKKHTNGKTLDAVL
                     AREGLGIALEMKAEKEKDAAKREQGLKDALAAFQAMQPDEKGPRYAYALYHQGRILAM
                     LGKTAEAKTQFEKAKEVGKDTKLPDLIDERLASLGA"
     gene            3593..4750
                     /locus_tag="VFV99_27285"
     CDS             3593..4750
                     /locus_tag="VFV99_27285"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5063121.1"
                     /translation="MKQYLALALVLVVSACVRVPPNRADAMSAEPREPLGEDRLSFKW
                     KFETADRRTEVTPQEFAAAAVYADTLYIGSAHGWFYALRASNGQVRWRRLVGSVVTAP
                     LVDRGMLYVGTNDGFLVALDAQTGAEKWRYQSRGPIGQTPAAAGDLIVFANEADQVVA
                     VDAISGKFKWQYKSETPEEYTLRGHAGVTIDGDLIYTGFSNGTLVALRRDSGSVAWST
                     SLKADADRFMDVDATPIVLEGTLYASSSSGGVYALDKATGLVKWRVPFYDASMPSATG
                     NVGGIASDGKAIYVSVADLGTYAIDLNGNILWRVGARGGGEPAEPVVFADMVVYSLAS
                     AGLFIAERKTGKTLEWFDPGDGVSAQPTITADGRLFMMSNRGILYAFDLEY"
     gene            complement(4747..5676)
                     /locus_tag="VFV99_27290"
     CDS             complement(4747..5676)
                     /locus_tag="VFV99_27290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063122.1"
                     /translation="MRTLALILLLAACDAGEKPAPPAPSPTPKKIPTAQSVPDAKYLP
                     ADSDLVVKLDLAALRRAKLWTTYGRQVSNLIVPGFSQCDYDPLAAARSIVIGIPLESQ
                     RAVYVVRGTDRNQAIGCLRTSAAVKLDGDFFTVTKANGNDYVFTFVDDTTMVMQAWQG
                     ATKQTLQRALAADVPTGDPAFAMMMARIAGAAPIAIASRPGSKTIEEKAATSGIRPQE
                     MYGTLTLTDGLHITYATVFKTKDEATQFANLMDAQLEQTKTYWEATAQAQGNTTTMSV
                     AMTEAQVTSMVNLLEPMLNTPPVPPAHTPEPID"
     gene            complement(5695..6855)
                     /locus_tag="VFV99_27295"
     CDS             complement(5695..6855)
                     /locus_tag="VFV99_27295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063123.1"
                     /translation="MRVWLLVAALAACSSESSDVIGPFSGANRKFIVDRIEVPVESTD
                     VTAYADDLDGDGADDNQFGNVTSVLATTNDLSTHMTDMIASGAITSIFMIYADNFTDD
                     DHVGVLYLGSADDSPNSAFAGGRFEAGGFSSNRTRDTRAPGRSRVHLPIYVNADPLAL
                     QLEGMEVDLTPDGGDGYDGFVRGGIAEDLARQAAFEGLVQMAETEPERHIVFLRGIDK
                     NHDDVITREEVDDSVIALLVTADIALFDGDHYGPHPSTVHDSLSVGFKIHLKLCPGGI
                     CTPLDPPVNLCRDRWQDGGETDIDCGGPCQPCAAAKHCNLPTDCQSNACDSGSCRAPS
                     CTDGVRDGYESDIDCGGSCPKCAAGLVCAADSDCANNNCDNGVASLGTCAGP"
     gene            complement(6869..7627)
                     /locus_tag="VFV99_27300"
     CDS             complement(6869..7627)
                     /locus_tag="VFV99_27300"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5063124.1"
                     /translation="MPAVLSRADEHARELRDLEDKLGRCIAAMTMHYQPIVHAATRSR
                     FGYEALLRSTDKSLPHPGAILDAAERLEKIPTLGRAVRAQVARAVAESPAERGVVFVN
                     LHLLDLFDKQLSSAFAPLTKVSQRVVLEITERTSLEGQADVRYRVQELRELGFKIAID
                     DLGGGHARMGTFSPLDFDYVKLDMSLVRDMDKHPMKQRLVKSIVELCKEHDTKVIGEG
                     VETEAEAKVLIDLGCDLLQGYLIARPALPFADPL"
     gene            complement(7841..8311)
                     /locus_tag="VFV99_27305"
     CDS             complement(7841..8311)
                     /locus_tag="VFV99_27305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063125.1"
                     /translation="MATAAYAVSPQTPISSEVSTTLSVSAGAAFEIFADAVEIPRWLP
                     LVQGARVLAMQPDGRPSRVAFTRKLERGSLGYTLEYRYDPSTYTITWSTPKTSNVVLT
                     GEARFVPLSTRACLMLYQLVLDLPIVDDLITTELDGHPASLVVAEFREHLRRLC"
     gene            complement(8448..9236)
                     /locus_tag="VFV99_27310"
     CDS             complement(8448..9236)
                     /locus_tag="VFV99_27310"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5063126.1"
                     /translation="MPEGDSIKRIAMQLAPLVGQTLERVTTQGIERDLAGRTITEVHP
                     HGKHLMIDLDDGTQLRAHLGMNGRVRRYSREDGEALIARMSPGRLSLVLVTASLVVMW
                     ISAPTIEIAARRAPMRGLAVASLGPDVLADGFDPQLAASRAHVHSARTIADVLLDQRV
                     AAGIGNIWKTESLFACGIDPRTPIGALTIDAIARVYASARELMLANLAPGGPRNRFAI
                     YSRTNHPCPRCSTLIMAYQLGDPPRWTWSCPHCQPRGGVRGVSS"
     gene            complement(9229..>11602)
                     /locus_tag="VFV99_27315"
     CDS             complement(9229..>11602)
                     /locus_tag="VFV99_27315"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5063127.1"
                     /translation="DVEHVLVEELARSSVFATHFRENAARALLLPRRRPGQRTPLWAQ
                     RLRAQQLMGVALRFPAFPITLETYREVLRDVFDLPALVDILKQVRSRAIRIEPAATET
                     ASPFARSLVFDYVAAFLYEGDAPLAERKAQALTLDRNLLRELIGGGELRDLLDPDVLN
                     EVELELQQLVEGRKARNADEIHDLLRRLGDLEIHEINARFEPEELNAKEPRTPRTPSE
                     EAGSETTLLGGLGALGSLAFILDQLIATGRAVRVRIAGRDRYIAAEDAARYRDGLGIN
                     LPRGIPAALLERAVAPLESLLARFARTHAPFVAEQPAARWGMPAAQVEPVLALMEARG
                     QLVRGELRPGGMGLDSCDPEVLRQLRRRTLAKLRAQVAPVGTSAYAAFLPRWHGLDQP
                     RRGLLALRDAIARLEGVALSFTELESRILPARILDYHPRMLDELGAAGELTWVGAGAL
                     GTKDGRVMLLRRDRARELAPEPTEIANHSPVHDAIIEHLRKAGASFLVAIEQAAGGNR
                     AAVTAALWDLVWAGIITNDTFAPLRSLAAPNTRRTSAHASFGGRWSLVESLGPPPTAT
                     ARAHAQATALLERWGIASRNGARADELPGGFATVADVLRAMEDAGTVRRGYFVEHLEG
                     AQFAWPGAIDRLREAPRGQAQRVDVLAAVDPANAWGSALPWPELRDPDARPARRTGAT
                     AVLVDGALALWLEPKARRIATAAGLPAETIELACAVGLPRVAAKQRRRELLIETIDGE
                     AAGSSPLARSLVAAGARIDYRGLVVRGTVTQVLPDPEPEDEATEDEDVDA"
BASE COUNT         2026 a         3823 c         3878 g         1875 t
ORIGIN      
        1 gtcgaggtcg tgccgatttc cgcggcgacc ggcgacaacg tcgatgcgct cgagcgcgtg
       61 atcgcgcagc gcctgcccgc aggaccgccg ctgttcccgc agggcatggt gaccgatcgg
      121 tcgccgcagt tcatcgcgca ggagatcatc cgcgagcagc tctatcacca gctcggcaag
      181 gagctgccgt acgcgtgcgc ggtgcaggtc gagacctgga ccgagcgcaa gggcgagctc
      241 gtgatcggcg gcgtgatcgt cgtcgagcgc gagtcgcaaa agccgatcgt cgtcggcaag
      301 gcgggctcgc gtatcaagga gctcggcatc gcggcacgga ccgcgctcgc tgccgcgctg
      361 ggcaaacccg tgcacttgtc gctgttcgtc aaggtgcgcg aggactggag tcagaccgag
      421 aaggatctgc gcgagctcgg ctacgggggc gacaagtgat cgcgccgctg cttgcggtga
      481 tcggccggcc gaacgtcggc aagtcgacgc tgttcaatcg gctcgtcggt ggccgtcccg
      541 cgctcgtcca cgatacgccg cacctcactc gcgatcgccg ctatggcgag accgactact
      601 tcggcaagga gctgcggctc gtcgacaccg gcggtctcga tccggaggct gcgaccgaag
      661 tgatcggcgc cggcattcac cgccaggcga tgcgcgcgat cgacgaagcg gacgcactgc
      721 tgctcgtcgt cgatggccgc gccggcgtct cgccgatcga tcacaagctc gcgaaggagc
      781 tccgcgcgac cagcaaaccg gtcttcctcg cggtcaacaa gatcgatcac ccgacgaccg
      841 accacctcgt ccacgagttc tacgagctcg gcctcggcaa cccgttcgcg atctcggccg
      901 cacacggccg cggcatcgac gcgttgctcg aagcgatcac cgacgccgtc gtgaagacgc
      961 cggtcgccga cgtcgggccg cccgatgatg aagaggacga tctctatgac gtcctcgcgg
     1021 aaggcgacga agcagcggaa gagaccgacg agatggcagc gaagcgcgcc gcgcgcgatg
     1081 ccgagccgct gcgcgtcgcg ttcgtcggcc gccccaacgc aggcaagtcg tcactgacca
     1141 accgcttgct cggcgaggag cgctcgctcg ttcacgatgt agctggcacc accacggatc
     1201 cggtcgacgc gaagttcacg atcggcggcc gcgagtacgt gctcgtcgat accgcaggca
     1261 tccggcgcaa agcacgcatc gacgaggaga tcgaaaagat ctcggtctcg atggcgatcg
     1321 gccagatcga gcgcgcggat gtcgccgtgc tcgtgatcga cgctggcatc gggccctcgg
     1381 agcaggacgc gcggatcgcc ggcaagatcg aggaagctgg ccgcgcgctc gtgatcgccc
     1441 tcaacaagag cgacctcgtg caaggcgagg aaaagaagtc tgcgctccgc gatctcacga
     1501 aggacacgtt tcactttctc gactgggcgc ccgtcgtgaa cctgtccgca cagcgcggcg
     1561 atggtgtcga tcggctgctc gacgtcgtcg atcaggtcgc gaaggagcac aagaagcgga
     1621 tcccgaccgc gcagctgaac cgcttcttcg ccgacgtgat cgaggagatg ccgccgccgc
     1681 tccacaaggg ctacgcggtc cgcatccact acatcacgca aggcgcaacc cgcccgccga
     1741 cgttcatctt gatggcgaac aagcccgagc tcgcgccgag ctacaagcgt ttcatcgcga
     1801 accgcttgcg caaggcgtac ggcttcaagg gcacgccgat ccgaatcttc gtgaaggccc
     1861 gcaaagaccg tcacgacacc gactagttac ttcttgatcc ggaacgcgcg cacgtaatac
     1921 gcgagcgccc agcggtctgc ctctttgatc gtcggatagc cgaccatctt cgtgttcggc
     1981 agccccgtcg acacggtctt gaagatctgc tcgacgccgt cgcccgcctt gaacggatcc
     2041 ttcaccagat tgcgcggcgg cggcttcacc gcaaacgcga ctggaccgtt gccctcgcca
     2101 gtcgcgccgt gacaggcggc gcagttgacg tcgtagacct gcttgccggt ctcgagcagc
     2161 ttcggcgtcg gcggtggcgc ggcggcaacc agcaccatgg caacgagcgc cacagcgacg
     2221 aatgctctca gaagtacccc cagcggatcg ttccgcgcag accgaacgtg aacatcgtga
     2281 cgtcgccgcc gaacgaggcc agccgcgcct cgactcccca cgatcgctcg cgactcgtca
     2341 gcacgtcgag cccaccgccg accgcgagca cgagactcgc gttgtcaccg atcggcccgc
     2401 cggtcgtgaa catcccaccg atcccgccga acgcgtacgg cacgtacttg agcacgtcga
     2461 agcgcagcac cgcgccgacg tcgatcaggc ccgcgtacga cagcgcggcc tggtccatct
     2521 tctggccgcc gtagaacaga ccgccggcta gctcgccgcg cagcgagaag tccgtgctga
     2581 tcgactgctc gtagatgcca gtcagggcgc cgccgaccgt aggcgtcacg ttggggggct
     2641 ccatgtttcc ctcggcgaca ccggggactg aaaacgtggc ccaggacagg ccgacggaca
     2701 gcgcgcgctc gccctctgcc cgggcctcac cgtagggcgc caggagcgca cagacgatca
     2761 cgggcaacag ttgggtcggt cgcagggtca gagccaccaa acccctgaat tgtgctacat
     2821 cgccaggcgc cgtggccgac aaggacaaca aaaaccccac caagcccgca gggacctccg
     2881 ccaagcccgg tttcgatccg aagccggtcc aggtcggcgg cgagtcgctg gtcgagcggc
     2941 ttatcccgca catgaagcaa atcgtgatcg ggatcctggt catcgctgtg gcgctgaccg
     3001 tggtcttcac ggttcgctac ttcaaagagc gcgggcgcca gaaggacact gccaagctcg
     3061 agcacgtgct cgacgtggcg tcacgtcagg tgcgcccgcc gggagtcgag ccggatccca
     3121 aggccaaaga gccgacgttc gcgaacaaca aggagcgcgc gatcgcggtc ctcgccgcag
     3181 cccaaaaaga gggcagcaag gcgacggagg gcgcattcaa ggcgtcgatg ttcgtccagg
     3241 ccgatcagct cgacgacgcg attgccgagt acaagaagca cacgaacggc aagacgctcg
     3301 acgccgtgct cgcgcgcgaa ggcctcggca tcgccctcga gatgaaggcg gaaaaagaga
     3361 aggacgcggc caagcgggag cagggcctca aggacgcgct agccgcgttc caggcgatgc
     3421 aacccgacga gaagggcccg cgctacgcgt atgcgctcta ccaccagggc cgcattctcg
     3481 cgatgctcgg caagaccgcc gaagcgaaga cacagttcga gaaagccaaa gaagtcggca
     3541 aggacaccaa gctccccgac ctgatcgacg aacgtctcgc gagcctcggc gcatgaaaca
     3601 gtatctagct ctcgccctcg tcctcgttgt gtccgcgtgc gtgcgtgtgc cgcccaatcg
     3661 cgcggatgcg atgagcgcag agccgagaga accgctcggc gaggatcggc tctcgttcaa
     3721 atggaagttc gagaccgctg atcgccgcac cgaggtcacg ccgcaagagt tcgcggccgc
     3781 cgccgtctac gcggacaccc tgtacatcgg ctcggcgcac ggctggtttt atgcgctgcg
     3841 cgcgtcgaac ggtcaggtgc gctggcgccg ccttgttggc tcggtcgtga ccgcgccgct
     3901 cgtcgatcgc ggcatgctct acgtcggcac caacgacggc ttcctcgtcg cgctcgacgc
     3961 ccagaccggc gccgagaagt ggcgctatca gagccgcggc ccgatcggcc agacaccggc
     4021 agcggccggt gatttgatcg tgttcgcgaa cgaggccgat caggttgtcg ccgtcgacgc
     4081 gatcagcggc aagttcaagt ggcagtacaa gagcgagacg cccgaggaat acacgctgcg
     4141 cggccacgcc ggcgtgacga tcgacggcga cctcatctac accggcttct cgaacggcac
     4201 gctcgtcgcg cttcgtcgcg atagcggctc ggttgcatgg tcgacgtcgc tcaaggccga
     4261 tgccgatcgc ttcatggacg tcgatgcgac gcccatcgtg ctggaaggca cgctgtacgc
     4321 gtcgtcgtcg tcgggcggtg tctacgcgct cgacaaggca accggcctcg tgaagtggcg
     4381 cgtgccgttc tacgacgcgt cgatgccgag cgcgaccggc aacgtcggcg gcatcgcgag
     4441 cgacggcaag gcgatctacg tctcggtcgc cgacctcggc acgtacgcga tcgatctcaa
     4501 cggcaacatc ctgtggcgcg tgggcgcgcg tggcggcggc gagccggccg aaccggtcgt
     4561 gttcgccgac atggtcgtgt actcgcttgc gagcgcgggc ctgttcatcg ccgagcgcaa
     4621 gaccggcaag acgctcgagt ggttcgatcc gggcgacggc gtctcggcgc agccgacgat
     4681 caccgccgac ggccggctgt tcatgatgtc gaaccgcggc atcctgtacg cgttcgacct
     4741 cgagtactaa tcgatcggct cgggcgtgtg cgccggcgga acgggtggcg tgttcagcat
     4801 cggctcgagc aagttgacca tcgaggtgac ctgcgcttcc gtcatcgcga cggacatcgt
     4861 cgtcgtgttg ccctgcgcct gcgcggtcgc ctcccagtac gtcttggtct gctcgagctg
     4921 ggcgtccatc aggtttgcga actgagtcgc ctcgtccttc gtcttgaaca cggtggcgta
     4981 ggtgatgtgc aggccgtcgg tcagcgtcag cgtgccgtac atctcctgcg ggcggatgcc
     5041 gctcgtcgcc gccttctctt cgatcgtctt gctgccgggc cgcgacgcga tcgcgatcgg
     5101 cgcggcgccc gcaatccgcg ccatcatcat cgcgaacgcc ggatcgccgg tgggcacatc
     5161 cgcagctagc gcgcgctgca gcgtttgctt cgtcgcccct tgccaggctt gcatcaccat
     5221 cgtcgtgtcg tcgacgaacg tgaacacgta gtcgttgcca ttcgccttcg tcaccgtgaa
     5281 gaagtcacca tcgagcttca cggcggcgct cgtgcgcagg catccaatcg cttgattgcg
     5341 atcggtgcca cgcacgacgt agaccgcgcg ctgcgactcg agcggaatgc cgatcacgat
     5401 cgatcgcgca gcggcgagcg gatcgtaatc gcactgcgag aagccgggca cgatcaggtt
     5461 cgagacctgg cggccgtagg tcgtccacaa cttcgcgcgg cgcagtgccg caaggtcgag
     5521 ctttacgacg agatccgagt cggccggcaa gtacttcgcg tccggcacac tctgggcggt
     5581 gggaattttc ttcggcgtcg gcgacggcgc gggcggcgct ggcttttcgc cggcatcgca
     5641 cgcagcaagc agcaggatca acgcgagggt gcgcacgcca ggttgtgaca cgggctatgg
     5701 gccggcgcag gttccaaggc tcgcaacacc gttatcgcag ttgttattcg cgcaatcgga
     5761 gtccgctgca cacacgagtc cggccgcgca cttggggcac gaaccgccgc agtcgatgtc
     5821 ggactcgtag ccgtcgcgaa caccgtcggt gcagctcggc gcgcgacacg atcccgagtc
     5881 gcaagcgttc gactggcagt ccgtcgggag gttgcagtgt ttggcagcag cgcacggctg
     5941 acacggaccg ccacagtcga tgtccgtctc gccgccatcc tgccagcgat ctcggcacag
     6001 gttcaccggc gggtccaatg gcgtgcagat gccgccggga catagcttca ggtgaatctt
     6061 gaagccgacc gacagcgaat cgtgaacggt cgacggatgc ggcccgtagt gatcgccgtc
     6121 gaacagcgcg atgtcggcag tgacgagcag cgcgatcacc gaatcgtcga cttcctcgcg
     6181 cgtgatcacg tcgtcgtgat tcttgtcgat accgcgcagg aacacgatat ggcgttcggg
     6241 ctcggtctcc gccatctgca cgagaccctc gaacgcggct tgccgcgcga gatcttcagc
     6301 aataccgccg cgcacgaagc cgtcgtagcc gtcgccgccg tcgggcgtga gatcgacctc
     6361 catgccttcg agctgcagcg cgagcggatc ggcgttgacg tagatcggca ggtgaacacg
     6421 cgacctgccg ggtgcgcggg tgtcgcgcgt acggttcgac gaaaaccccc ctgcctcgaa
     6481 gcgaccgccg gcgaatgccg aattcgggct gtcgtcggcg gaaccgaggt acaacacgcc
     6541 gacgtggtcg tcgtcggtga agttgtcggc gtagatcatg aagatcgacg tgatcgcacc
     6601 ggacgcgatc atgtcggtca tgtgcgtcga tagatcgttc gtggttgcaa gcaccgaggt
     6661 gacgttgccg aactggttgt cgtccgcgcc atcgccatcg aggtcatcgg cgtaggccgt
     6721 cacgtcggtg ctctcgactg gcacctcaat ccgatcgacg atgaacttgc ggttagcgcc
     6781 ggagaacggt ccgatgacgt cgctcgattc agacgagcac gcagccagcg cagcgacgag
     6841 aagccaaacc ctcaccagct cggttgtatc agagcggatc ggcgaacggc agcgctggtc
     6901 tcgcgatcag gtagccctgg agcaggtcgc agccgagatc gatcaacacc ttggcctcgg
     6961 cctcggtctc gacgccttcg ccgatcacct tggtgtcgtg ctctttgcac agctcgacga
     7021 tcgacttcac gaggcgctgc ttcatcgggt gcttgtccat gtcgcgcacg agcgacatgt
     7081 cgagcttcac gtaatcgaag tcgagcggcg agaacgtgcc catgcgcgcg tgaccgccac
     7141 cgagatcgtc gatcgcgatc ttgaagccga gctcgcgcag ctcctgcacg cgatagcgga
     7201 catcggcttg gccttcgagc gacgtgcgct cggtgatctc gagcaccacg cgctgcgaaa
     7261 ctttcgtgag cggcgcgaac gccgacgaca gctgcttgtc gaacagatcg agcaggtgca
     7321 ggttcacgaa cacgacgccg cgctccgcgg gcgactcggc gaccgcgcgc gcgacttgtg
     7381 cgcgcactgc gcggccgagt gtcgggatct tttcgagacg ttcggcggcg tcgaggatcg
     7441 cgccgggatg cgggagcgac ttgtcggtcg atcgcagcag cgcttcgtag ccgaaacgcg
     7501 aacgcgtcgc ggcatgcacg atcggttgat agtgcatcgt catcgccgcg atgcagcgcc
     7561 cgagcttgtc ttcgagatct cgcagctccc gcgcgtgctc gtcggctctg ctcaagaccg
     7621 ctggcaccgc cggctgcgta ccgactgcaa actcgttgag gtcgtcgttt ggagtcggtg
     7681 gtcgcgtgcg caagctcgct ggaagcggtg gtacatctct gggagtagga cgccccgacg
     7741 acacgatttc tcatcgtata caggccggct tgcagaagcc aatgccgtca attgttgcac
     7801 ccagggctaa ctaccccacc gcataccgaa caatgtaggc ctagcagagg cgacggaggt
     7861 gttcgcggaa ctcggcgacg acgagcgagg ccgggtggcc gtcgagctcg gtcgtgatca
     7921 aatcatcgac gatcggcaga tcgagaacga gctgatagag catcaggcac gcgcgcgtcg
     7981 acagcggaac gaaccgcgcc tcgccggtca ggacgacgtt cgaggtcttc ggcgtcgacc
     8041 acgtgatggt gtaggtgctg gggtcgtagc gatactcgag cgtgtagccg agcgacccgc
     8101 gctcgagctt gcgcgtgaat gccacgcggc tcggccgacc gtcgggctgc atcgcgagga
     8161 cgcgcgcgcc ctggaccagc ggcaaccagc gcggaatctc gaccgcgtcg gcgaagatct
     8221 cgaaggcggc tccggcgctg acgctaagcg tcgtcgagac ctcactggag atgggggtct
     8281 gcggactgac tgcgtatgcg gcggtggcca tgggcgataa ctccttcgtg cccggtcgca
     8341 tgagcaaccc ggataccagc ggcaagcgac ggggttccgc gtagttgaat agcgttctgc
     8401 cccgtcgcag ctgaccactc gaatagccgc ggcgcgatcg gtttccgcta actcgagacc
     8461 ccacgaacac ccccacgcgg ttggcagtgc ggacacgacc acgtccaacg cggtgggtca
     8521 ccgagctggt acgccatgat gagcgtgctg cagcgcgggc agggatggtt cgtgcggctg
     8581 tagatcgcaa acctgttgcg cgggccaccc ggcgccaagt tcgcgagcat cagctcacgc
     8641 gccgatgcat agacacgtgc gatcgcgtcg atcgtgagtg cgccgatcgg cgtgcgcgga
     8701 tcgatgccgc atgcgaacag cgactcggtc ttccagatgt tgccgatgcc ggccgcgacg
     8761 cgttgatcga gcaggacgtc ggcgatcgtc cgcgcagaat gcacgtgcgc gcgtgacgcg
     8821 gcgagctgtg gatcgaagcc atcggcgagg acatccgggc ccagcgacgc caccgcaagc
     8881 ccacgcatcg gcgcgcgccg cgctgcaatt tcgatcgtcg gcgcgctgat ccacatcacg
     8941 acaaggctcg cggtgacgag cacgagcgac agccggcccg gtgacattcg cgcgatcaat
     9001 gcttcgccgt cttcgcgcga atagcggcgc acgcgaccat tcatgccgag atgcgcgcgc
     9061 agctgcgtgc catcgtcgag atcgatcatc aggtgcttgc cgtgcggatg cacctccgtg
     9121 atcgtgcggc cggcgagatc gcgctcgatg ccttgcgtcg tcacccgctc gagcgtctgg
     9181 ccgacgagcg gcgcgagctg catcgcgatg cgcttgatgc tgtcgccttc aggcatcgac
     9241 gtcttcgtct tcggtcgctt cgtcttcagg ctctggatcc ggaagaacct gcgtgaccgt
     9301 gccgcgcacg acgaggccgc gataatcgat gcgcgcgccc gcggcaacga ggctgcgtgc
     9361 gagtggtgag ctgccagcgg cttcgccgtc gatcgtttcg atcaatagct cgcgacggcg
     9421 ttgcttggcg gcgacgcgcg gcaagccaac cgcgcacgcg agctcgatcg tctcggcggg
     9481 aagcccggcg gcggtcgcga tgcgccgtgc cttcggctcg agccacagcg cgagcgcgcc
     9541 atcgacgagc accgcggtgg cgccggtgcg gcgtgccggc cgcgcatctg gatcgcgcag
     9601 ctcgggccag ggcagggcgc tgccccacgc gttggccgga tcaaccgcag cgagcacatc
     9661 gacgcgctga gcctggccgc gtggcgcttc acgcaagcga tcgatcgcac ccggccatgc
     9721 gaactgcgca ccttcgaggt gctcgacgaa gtagccgcgg cgcacggtgc cggcgtcttc
     9781 catcgcgcgc agcacgtcag ccaccgtcgc gaagccgcct ggcagctcgt cggcgcgcgc
     9841 accgttacgg ctcgcgatgc cccagcgctc gagcagcgcg gttgcttgcg cgtgtgcgcg
     9901 agcggttgcg gtcggcggtg gaccgagcga ttcgacgagc gaccaccggc caccgaagct
     9961 cgcgtgcgcg ctcgtgcgtc gtgtattcgg tgcggcgagc gagcgcagcg gcgcgaacgt
    10021 gtcgttcgtg atgatgccgg cccacacgag atcccacagt gcggcggtca cggcggcgcg
    10081 gttgccgccc gcggcttgct cgatcgcgac aaggaacgat gcacctgctt tgcgcaggtg
    10141 ctcgatgatc gcgtcgtgca ccggtgagtg gttcgcgatc tcggtcggtt cgggcgcgag
    10201 ctcgcgtgcg cggtcgcggc ggagcagcat cacgcggccg tcttttgtgc cgagcgcacc
    10261 ggcgccgacc caggtcagct cgcccgcggc accgagctcg tcgagcatgc gcgggtgata
    10321 atcgaggatg cgcgcgggca ggatgcggga ctcgagctcg gtgaacgaca gcgcgacacc
    10381 ttcgagccgc gcgatcgcat cgcgcagcgc gagcagacca cggcgcggct gatcgagccc
    10441 atgccagcgc ggcaagaacg cggcgtatgc gctggtaccg accggcgcga cctgcgcgcg
    10501 cagcttcgcg agcgtgcggc ggcgcagctg gcgcagtact tcggggtcgc agctatcgag
    10561 gcccatgcct cccggccgca attcgccgcg cacgagctgg ccgcgtgctt ccatgagcgc
    10621 cagcactggc tctacctgtg cagcaggcat gccccacctg gcggcgggct gctcggcgac
    10681 gaatggcgcg tgcgtccgcg caaagcgagc gagaagggac tcgagcggcg cgacggcacg
    10741 ctccaggagt gcggccggaa tccccctcgg caaattgata ccgagaccgt cacgatatcg
    10801 cgcagcatct tcagccgcga tgtatcggtc gcgacctgcg atgcggacgc gtacggcacg
    10861 gcctgtcgcg atgagctgat ccagaataaa cgccaaggag ccaagagcgc caaggccgcc
    10921 aaggagagtg gtttcggaac ccgcttcttc gcttggcgtt cttggcgtcc ttggttcctt
    10981 ggcgttcaat tcttcgggtt cgaaccgcgc gttgatctcg tgaatttcga ggtcgccgag
    11041 gcgacgtagc aggtcgtgaa tctcgtccgc attgcgtgct ttgcgacctt caacgagttg
    11101 ttggagctcg agctcgactt cgttaagcac atcgggatcg agcaagtcgc gcagctcgcc
    11161 accgccgatc agctcgcgga gcaagttgcg gtcgagcgtg agcgcctgtg ctttgcgttc
    11221 ggcgagcggc gcgtcgcctt cgtagagaaa cgccgcgacg taatcgaaca cgagcgagcg
    11281 cgcgaatggc gacgcggtct cggtggccgc gggctcgata cggatcgcgc gcgagcggac
    11341 ctgtttcaag atgtcgacca gcgccggtag atcgaacaca tcacgcaaga cctcgcgata
    11401 ggtctcgagc gtgatcggga acgcggggaa ccggagcgcg acgcccatca gttgttgcgc
    11461 gcggaggcgc tgcgcccaca gcggcgtgcg ctggccgggg cgccggcgcg gcaggagcag
    11521 cgcgcgcgcg gcgttctcgc ggaagtgcgt cgcgaacacg ctcgagcgcg cgagctcctc
    11581 gaccagcacg tgctcgacgt cg
//
LOCUS       DASUFX010000677         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_663865,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000677 DASUFX010000000
VERSION     DASUFX010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663865"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFV99_27320"
     CDS             complement(<1..905)
                     /locus_tag="VFV99_27320"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5063128.1"
                     /translation="MKRALLVMLAACSAAPRTTQPVRHAPTDTSPGLVVLVIVDQFPE
                     WSLERKRTELTHGFARILGEGDWRVGRHPSAATITAPGHTLLGTGRTPDASGIIANEW
                     WNRELNKKQKASEDENGQTSSKWLRVPGLGDAVAAAHTGAKAVAVALKNRAARLPLGH
                     AGTAIYYDAKTASFASNGNPTPWLATYNREHPVGPRLVPWVPLDPVRLEKLSGVPDAQ
                     KGEVGEKGFGPTFPHDPNTTKNAADAAYAMPLGNDLVLETALAAIDNEKLGDDRTVDL
                     LIVSLSSHDYIAHGWGHESWETWDS"
     gene            963..1790
                     /locus_tag="VFV99_27325"
     CDS             963..1790
                     /locus_tag="VFV99_27325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063129.1"
                     /translation="MARVAQLGGSPRALLLFALPIATLACASGHGSTTADGATPDSTH
                     PVDAAPADVTHVTADASVDATTPDAGCAISNGAVPALDGNNDIAKYTAAQQLAPGAMI
                     GSDAAALAWDRENLYVTVSSIAFGSAYEPLHVYVETGMTLGTAAAAHGKEYSGLTPAL
                     PFTPTQVIAVRRISNAGTGGYDGVFVPTDQWQARTIDLDSATYAASTQLSVRVPWSAL
                     GGCPTQMRLALHVVHGVAGNEWKDLVPSTHTPWVMPGGGYYEIDLTGAAAVASFTLR"
     gene            complement(1782..2255)
                     /gene="dps"
                     /locus_tag="VFV99_27330"
                     /gene_synonym="pexB"
     CDS             complement(1782..2255)
                     /gene="dps"
                     /locus_tag="VFV99_27330"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEX5063130.1"
                     /translation="MYKTPSQLPENSRIALIATLNDRLADGLDLHSQVKVAHWNVRGP
                     QFPSLHPLFEQFAIQLAAHNDAIAERAVTLGGLASGTTRYVAKHSRLGDYPQGITRDI
                     EHVALLADRIDTYLGGVRESRTIAERLGDTDTVDLLTGVVTEFEKNAWFLRATLA"
     gene            complement(2328..2957)
                     /locus_tag="VFV99_27335"
     CDS             complement(2328..2957)
                     /locus_tag="VFV99_27335"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5063131.1"
                     /translation="MIVACTNCKSRFEAAGYRDVLCPTCGSIAQQATVRPCPRCELPL
                     AARTVSDLVIDECTGCQGLFLDEVAIRLVLDDEDQSRAEALLDSLPRAAHRALPTAGA
                     RMYIKCPTCSTLMNRRLFATGSGVVVDVCKAHGTYFDAGELPAIIDFVRAGGLEIAAK
                     KDAKRKAERDERDRREAERVHDMPGGMREGTRRSDKGSALVDLLFALFG"
     gene            complement(2979..3239)
                     /locus_tag="VFV99_27340"
     CDS             complement(2979..3239)
                     /locus_tag="VFV99_27340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063132.1"
                     /translation="MSPTGAGIVLDVCKEHGAFFAAGDVARLIAFVCREARQRAAELA
                     EDPYAAQRVVPEEPPRYDNRWGAYEPSSPTEIARSILRAIGL"
     gene            complement(3251..3562)
                     /locus_tag="VFV99_27345"
     CDS             complement(3251..3562)
                     /locus_tag="VFV99_27345"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5063133.1"
                     /translation="MHVRCGACSHRFDADGYRDLRCPACQMLVSPLPGQCPSCAEPLT
                     SRELAGVAYEECDRCEGTFLTHAAIDALLTAAPKQSLCTARASLGRAFDRKRAVQCAR
                     M"
     gene            complement(3562..>4193)
                     /locus_tag="VFV99_27350"
     CDS             complement(3562..>4193)
                     /locus_tag="VFV99_27350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063134.1"
                     /translation="PRTAVNGKPLLNTYKEWLEGPYMRRGIQCQHCHMPNREHQWLGV
                     HDAATFRQGIELDARTIRKGGAVTVIATLTNIGAGHYLPTTTTPAAWLKLELFDASGV
                     AVAGANAAQRIGRDLVYDKQWVEKSDTRIPPGESLKLVRAWQGGRTAQAAYARVTVEV
                     HPDDYYERFYAARLKVKLPDATRKQYEVALQRAIGSHYLAEQRDVPIMK"
BASE COUNT          639 a         1412 c         1441 g          701 t
ORIGIN      
        1 tcgctatccc acgtttccca tgattcgtgg ccccagccgt gcgcgatata gtcgtgcgaa
       61 gacaggctga cgatcagtag atcgaccgtg cgatcgtcgc cgagcttctc gttgtcgatc
      121 gcggcgagcg cggtctcgag cacgagatcg ttgccgagcg gcatcgcata cgcggcgtcg
      181 gcagcgttct tcgtcgtgtt cggatcgtgc ggaaacgtcg gcccgaatcc cttctcgccg
      241 acttcgccct tctgcgcgtc aggcacaccg ctcagcttct cgagccggac cggatcgagc
      301 ggcacccacg gcacgagccg cggaccgacc ggatgctcgc ggttgtacgt cgcaagccac
      361 ggcgtcgggt tgccgttgct cgcgaagctg gcggtcttcg cgtcgtagta gatcgccgtg
      421 cccgcgtgac cgagcggcaa ccgggccgcg cgattcttca gcgcgaccgc aaccgccttc
      481 gcaccggtgt gcgccgcagc gaccgcatcg ccgagtcccg gcacgcgcag ccacttgctg
      541 ctcgtctggc cgttctcgtc ctcggacgcc ttctgcttct tgttgagctc gcggttccac
      601 cactcgttcg cgatgatgcc gctcgcgtcg ggcgtgcgac cggtgccgag cagcgtgtga
      661 ccgggcgcgg tgatcgtcgc cgccgacgga tggcggccga cgcgccagtc gccctcgccg
      721 aggatgcgcg cgaagccgtg cgtgagctcg gtgcgcttgc gctcgaggct ccactccggg
      781 aactgatcga cgatcacgag cacgacgaga cccgggctcg tgtcggtcgg cgcgtgacgc
      841 acgggctggg tggttcgcgg cgcggcagag caggcggcca gcatcacgag gagagcgcgc
      901 ttcacgggcg gtcacgatac tcgctttgcg tggcacgagc gctgctcagg agtgacgact
      961 gtatggctcg cgtcgcgcaa ctcggcggat cgccacgtgc cctcctgttg ttcgcgttgc
     1021 cgatcgcaac gctggcgtgc gcgagtggtc acggctccac gacagccgat ggcgcgaccc
     1081 cagattcgac gcacccggtc gacgcggcac ccgctgacgt cacgcatgtc acggccgatg
     1141 cttcggtcga tgcgacgacc ccggatgcag gctgcgcgat ctcgaacggc gcggtgccgg
     1201 cgctcgacgg caacaacgac atcgcgaagt acacagcggc gcagcagctc gcgccgggcg
     1261 cgatgatcgg ctccgacgcg gcggcactcg cgtgggaccg cgagaacctc tacgtcacgg
     1321 tcagctcgat cgcgttcggc tccgcttacg aaccgctcca cgtgtacgtc gagaccggca
     1381 tgacgctcgg caccgcagcc gcggcacacg gcaaggagta cagcggcctc acgccagcgc
     1441 tgccgttcac gccgacgcag gtgatcgcgg tgcgccgcat cagcaacgcg ggcaccggtg
     1501 gctacgacgg cgtgttcgtg ccgaccgatc agtggcaggc gcgcacgatc gatctcgaca
     1561 gcgcgacgta cgccgcgagc acgcagctgt cggtgcgcgt gccgtggtca gcgctcggcg
     1621 gctgcccgac gcagatgcgc ctcgcgctcc acgtcgtgca cggcgtcgcg ggcaacgagt
     1681 ggaaggacct cgtgccgtcg acccacacgc cgtgggtgat gcccggcggc ggctactacg
     1741 agatcgatct caccggcgcg gcggcggtcg cgagcttcac gctacgctag cgtcgcgcgc
     1801 aagaaccacg cgttcttctc gaactcggtg acgacgccgg tcagcagatc gaccgtgtcg
     1861 gtgtcgccga gccgctccgc gatcgttcgc gattcgcgca ccccgccgag gtaggtgtcg
     1921 atgcgatcgg cgagcagcgc gacgtgctcg atgtcgcgcg tgatgccttg cgggtaatcg
     1981 ccgagccgcg agtgcttcgc gacatagcgc gtggtgccgc tcgcgaggcc gccgagggtg
     2041 accgcgcgtt cggcgatcgc gtcgttgtgt gcggcgagct gaatcgcgaa ctgctcgaac
     2101 agcggatgca gtgacgggaa ctgcgggccg cgcacgttcc agtgcgcgac tttcacctgc
     2161 gagtggaggt cgagaccgtc ggcaagccgg tcattgagcg tcgcgatcag cgcgatgcgg
     2221 ctgttctcgg gcagctgact aggcgtcttg tacatgcgga caacctaagc gcagcccggt
     2281 gattcggata gctgctgaat gcgaatgact ctgataggcc aggcctatca gccaaacagc
     2341 gcgaacagca ggtcgacaag cgcgctgcct ttatcgctgc ggcgcgtgcc ctcgcgcatg
     2401 ccgccgggca tgtcgtggac gcgctcggcc tcgcggcggt cacgctcgtc gcgctcggct
     2461 ttgcgcttgg catccttctt cgcggcaatc tcgaggccgc cggcgcggac gaagtcgatg
     2521 atcgcgggca gctcaccagc gtcgaagtac gtgccgtgcg ctttgcagac gtcgacgacg
     2581 acgccggagc cggtcgcgaa cagccggcga ttcatgagcg tgctgcaggt cgggcacttg
     2641 atgtacatgc gcgcgccggc ggttggcagc gcgcggtggg cagcgcgggg cagcgagtcg
     2701 agcagagcct cggcgcgcga ttggtcctcg tcatcgagca cgaggcgaat cgcgacctcg
     2761 tcgagaaaca ggccctggca gccagtgcac tcgtcgatga cgagatcgct gaccgtgcgt
     2821 gcggcgagtg gcagctcgca gcgcggacac ggccgcaccg tcgcttgctg cgcgatcgag
     2881 ccgcacgttg gacacagcac gtcgcgatag ccggccgcct cgaaccggct cttgcagttg
     2941 gtgcacgcga cgatcatcga tctcattctg acacgacgtc acagtccgat cgcgcgcagg
     3001 atcgagcgcg caatctcggt cggcgacgac ggttcgtatg ctccccagcg gttgtcgtag
     3061 cgcggcggct cctccggcac gacgcgttgc gcggcgtacg ggtcttcggc cagctcggcg
     3121 gcgcgctggc gggcctcgcg acagacgaac gcgatcagcc gcgcgacatc gccggccgcg
     3181 aagaatgcgc cgtgctcctt gcagacgtcg agcacgatgc cagccccggt cggagacaag
     3241 cgtcgagcca tcacatccgt gcacactgga cagcgcgctt gcggtcgaat gctcggccga
     3301 ggctcgcgcg ggcggtgcag agcgactgct tcggcgctgc ggtgagcaat gcgtcgatcg
     3361 cggcatgcgt caggaacgtg ccctcgcagc ggtcgcactc ttcatacgcg actccagcaa
     3421 gctcgcgcga cgtcagcggc tccgcacagc tcgggcactg accgggcagc ggcgacacga
     3481 gcatctggca tgccgggcac cgcagatcgc gataaccatc cgcatcgaag cggtgactac
     3541 atgccccgca cctgacgtgc atcacttcat gatgggcacg tcgcgttgct ccgcaaggta
     3601 gtgcgagccg attgcccgct gtagtgccac ctcgtactgc ttgcgcgttg cgtctggcag
     3661 cttcaccttg aggcgcgcgg cgtagaagcg ctcgtagtaa tcgtcggggt gcacctcgac
     3721 ggtgacgcgc gcgtacgcgg cttgtgcggt tcgcccgcct tgccacgcgc gcacgagctt
     3781 cagtgactcg ccaggcggaa tgcgcgtgtc gctcttctcg acccactgct tgtcatagac
     3841 gaggtcgcgg ccgatccgct gcgcggcgtt cgcacctgcg accgcgacgc cgctcgcgtc
     3901 gaacagctcg agcttcagcc acgcggcggg tgtggtcgtc gtcggcaggt aatgcccggc
     3961 gccaatgttg gtcagcgtcg cgatcaccgt gaccgcgcca cccttgcgga tcgtgcgcgc
     4021 gtcgagctcg atgccctggc ggaacgtggc cgcatcgtga acgccgagcc attggtgctc
     4081 gcgattcggc atgtggcagt gctggcattg gatgccgcgc cgcatgtacg gaccttcgag
     4141 ccactcctta taggtgttga gaagcggctt gccgttgacc gcggtgcgcg gcg
//
LOCUS       DASUFX010000678         7977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2054550, whole genome shotgun sequence.
ACCESSION   DASUFX010000678 DASUFX010000000
VERSION     DASUFX010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054550"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFV99_27355"
     CDS             <1..186
                     /locus_tag="VFV99_27355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063135.1"
                     /translation="NGCWTGYCIPYAQCDQLPACSDLNEMDCIGRTDCSPTYEGHNCT
                     CNATSCTCQSWTFDSCK"
     gene            complement(193..417)
                     /locus_tag="VFV99_27360"
     CDS             complement(193..417)
                     /locus_tag="VFV99_27360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063136.1"
                     /translation="MRALPEGAFLPQGGGPPDGHRCAGEALIQFVMPAFLGWFTKRYD
                     VAWSAQDVTPGGSGLGPLPKSGVRVTITPR"
     gene            complement(437..784)
                     /locus_tag="VFV99_27365"
     CDS             complement(437..784)
                     /locus_tag="VFV99_27365"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5063137.1"
                     /translation="MRQEPPGSMGAPFLGEALAFLKDPFTFTLTRTRQHGNVWKTRIL
                     GDTVVFFAGPKAFSFFMNEDNFTRDNGSPKFLQEILHRDAVPFIDGARHKTRKRLLLT
                     AFTHDALDSYLPG"
     gene            complement(821..1648)
                     /locus_tag="VFV99_27370"
     CDS             complement(821..1648)
                     /locus_tag="VFV99_27370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Synerg-CTERM sorting domain-containing protein"
                     /protein_id="HEX5063138.1"
                     /translation="MKIIIVLLLLAAPLVAHAQTFPADTAYRPLRCGNAVMTDGFGDD
                     NANRDERDVVGDVAAPAGLRASDGTYLYLRMRLEDDPAPGGAVRTSSWGMEFDLDNDL
                     TDYEVLVLVEGLSGAAGNVQVFANTQTTMPNSPADPADTPPAQTYMFASNARTVAAAG
                     SNFGGNADFFLDFAVPWSALTPLGLDRDTPVRVWVASSTLPDALNGDFACHAGGGGTP
                     PLDGGTASDPTTGDPNDPGANAGTGKLEGGGGCSAGAGAGWLVLLAVPLVWRKRRSV"
     gene            complement(1645..3078)
                     /locus_tag="VFV99_27375"
     CDS             complement(1645..3078)
                     /locus_tag="VFV99_27375"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063139.1"
                     /translation="MRGLAATLLVAMCGTAAGQGLDAERFRPATGAEGGFVNEHPAVP
                     FHLGWSLGLFLNYADDQVVERDDVTGAILSTPVHRALTADVVGSIGLFSRLELGLGLP
                     IHLIYDGDPYGGLAAGPGVGDLYFVPKVAIIRSGSLDKHVLLGFALPITFPTGNDEEF
                     RGAGGFTVAPELLFAAHLNQLGLGLDVGYRWRAEHPQLPYGDSITLGPWVSYNFTDEL
                     SLRAEMFAEKHVNTEVDGADFPIEVLGGVEYRMGGLALYGGASFGLTDGIGDPDFRII
                     GGVRYRKNHPDRQGFKDSDGDGVLDKDDKAPYEPEDEDGYQDDDGKPEPDNDGDGVLD
                     GDDECPELKGEKDRDGCPSHTYVKIEDGQMIIIGKIQFETGSAHVHKRSGPLLDQIAQ
                     ALDAHPQIKKVRIEGNTDNVGPPPLNQKLSEDRAASVKDELSKRGVDGDRLVTKGNGE
                     TRPIAPNKSAGGRQKNRRVEFFIQESK"
     gene            complement(3215..4246)
                     /locus_tag="VFV99_27380"
     CDS             complement(3215..4246)
                     /locus_tag="VFV99_27380"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5063140.1"
                     /translation="MTMTAMVLREHGGPDVLHLEQLPVPEPGPGEVRVRIRAVALNHL
                     DIWVRKGGPAFKLEYPHRLGSDIAGTIDALGPGAKGDVGANVVVQPGLSCGKCAACLG
                     GHDNLCRFYKILGENTQGGYAEYIVVPQVNIAPYPERLTFPQAAASILPFLTAWQMVV
                     HKARVQPGDTVLVQGAGSGIGVAAIQIAKLHGARVITTASTDDKLERARALGADITID
                     YTKTDFIAECKKLTDKRGVDAVIEHVGGDVFVGSIKAVRNGGRVVTCGATAGFHPAID
                     LRHIFFRQVEVLGSTMGSKADLLAVLAHVAAGRLNPVVHEVLPLARAADAHRLLEDRK
                     AFGKVVLEP"
     gene            4275..4979
                     /gene="gloB"
                     /locus_tag="VFV99_27385"
     CDS             4275..4979
                     /gene="gloB"
                     /locus_tag="VFV99_27385"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX5063141.1"
                     /translation="MRVAAIPCLSDNYAYLVIDGTQAAIVDPSEAPPVLAAIERLGVT
                     PAAIWLTHHHHDHVGGIEGIVAKYPNVEVVGHTSDKARIPKVTKLVNEGDVVTLGSLK
                     AHIIHNPGHTLGAISYWIKDGDGAVFTGDTLFGAGCGRIFEGTPPMMHESLHKLAALP
                     PTTHVYFGHEYTAANLKFAVYVEPNNTAVAARLETVTTPSTPSTIASERETNPFLLAK
                     SVDEFAARREAKNSFK"
     gene            complement(4972..5898)
                     /locus_tag="VFV99_27390"
     CDS             complement(4972..5898)
                     /locus_tag="VFV99_27390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5063142.1"
                     /translation="MVVSDPLPSWHDRGAKQAIVEFVTRVTRDRHADFVPPQERIAVF
                     DHDGTMWVEQPMYTQLAFAIDRVKALAPAYPEWHAQQPFKAALEDNLAALAATGERGA
                     AELVAATHANDTTDEFTEIVIGWLMNARHPTLRRLYTELVYQPMVDMLAYLRANGFTT
                     FIVSGGGVELIRPWTQRVYGVPPEQVIGSRVKLHYEYRGNRPVLYRLPEIEPVDDRAA
                     KPAAIYEAIGRRPILAFGNSDADFEMLDWTTAGDGPRLGVLVHHTDAAREFAYDRDTH
                     FGRLSRAVDAAPDRNWVVVDMARDWREMFAAT"
     gene            complement(5900..7444)
                     /locus_tag="VFV99_27395"
     CDS             complement(5900..7444)
                     /locus_tag="VFV99_27395"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5063143.1"
                     /translation="MANSNAAKKPNILVIFGDDIGIWNLSCYSSGMMGYQTPNIDRIA
                     REGMRFTDLYGEQSCTAGRASFMTGQSVYRTGLSKVGMPGAPVGLHAEDVTIAELLKP
                     LGYATGQFGKNHFGDRNEYLPTVHGFDEFFGNLYHLNAEEDPEDPDYPTNPEFRAKFL
                     PRGVIKSWATDKDDTTTDKRWGKVGKQKIEDTGPLNRKRMETCDDEFVAAAQDFIQRQ
                     TKANKPWFVWLNTTHMHFRTHPKPSSVGQAGRSQSPYHDTMIDHDKQIGSMLDQLDKL
                     GIASETIVLYTTDNGPHMNSWPDGAMTPFRSEKNTNWEGAFRVPGVLRWPGKIAAGSI
                     SNEIVSHHDWLPTFLAAAGEPHIMEKLRSGYSAVGKTFKNHIDGYNLLGYLTGKDKTS
                     SRPGFIYFSDDGDLVALRYGNWKAVFLEQRRRGTLEVWGEPFTKLRIPKLFNLRTDPF
                     ERADVTSNTYWDWYLSKAYMIMAAQSIVTKFLETFKEFPPRQKAASFTIDQAMQAMEA
                     SMTEGR"
     gene            complement(7594..>7977)
                     /locus_tag="VFV99_27400"
     CDS             complement(7594..>7977)
                     /locus_tag="VFV99_27400"
                     /inference="COORDINATES: protein motif:HMM:NF044447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063144.1"
                     /translation="SLLQFRAAHPALHPEKWIEPTQVSWRDAAGQVVSGGYMDDATKP
                     ALAWRLDGPSFGDQTLYIVYNHSAQVVRVTLPAPPSGLAWYRAGDTSAFMEAQNNFHA
                     PGEEYRMNQSLYDLGARAVAIFVAK"
BASE COUNT         1354 a         2639 c         2582 g         1402 t
ORIGIN      
        1 aacggctgct ggaccggcta ctgcattccg tacgcgcaat gcgaccagct gcccgcgtgc
       61 agcgatctga acgagatgga ctgcatcggg cgcaccgatt gctcgccgac gtacgagggc
      121 cacaactgca cgtgcaacgc cacgagctgc acgtgccaga gctggacgtt cgacagctgc
      181 aagtgagtcg agtcagcgcg gcgtgatcgt cacgcgcacg ccgctcttcg gcagcgggcc
      241 gaggccactg ccgcccggcg tcacgtcctg cgcagaccac gccacgtcgt agcgcttcgt
      301 gaaccagccg aggaacgccg gcatcacgaa ctggatcagc gcttcgccag cgcagcgatg
      361 gccgtcgggt gggccgccac cttggggcaa gaacgcaccc tcgggcagcg cgcgcacggc
      421 gtcgtcgccg agccggtcat cctggcaagt agctgtcgag cgcgtcgtgc gtgaacgcgg
      481 tcagcagcag gcgcttgcgt gtcttgtggc gagctccgtc gatgaacggc accgcgtcgc
      541 gatgcaggat ctcctgcagg aacttcggcg agccattgtc gcgcgtgaag ttgtcctcgt
      601 tcatgaagaa cgagaacgcc ttcggaccgg cgaagaacac gacggtgtcg ccgaggatcc
      661 gcgtcttcca cacgttgccg tgctgcctcg tgcgcgtcag cgtgaacgtg aacggatcct
      721 tgagaaacgc gagcgcttcg ccgaggaacg gcgcacccat cgacccggga ggctcttgac
      781 gcatgggcct gtcatcgtac gagagagccg gcgccgcaga tcacaccgag cggcgttttc
      841 gccacacgag cggcacggcc aggagcacga gccagcccgc accggcaccg gccgaacagc
      901 cgccaccacc ttcgagcttg ccggtccccg cgttcgcacc ggggtcgttt ggatcaccgg
      961 tcgtcggatc ggacgcagta ccaccatcga gcggcggcgt gccgccacca cccgcgtgac
     1021 atgcgaagtc gccgttgagt gcgtcgggca gcgtcgagct cgcgacccac acgcgcaccg
     1081 gcgtgtcgcg atcgaggccg agcggcgtga gcgcgctcca cggcaccgcg aagtcgagga
     1141 agaagtcagc gttgccgccg aagttgctcc ccgccgcagc gacggtgcgc gcgttgctcg
     1201 cgaacatgta cgtctgcgcg ggcggcgtat ccgccggatc cgctggactg ttcggcatcg
     1261 tggtctgcgt gttcgcaaat acctgcacat tgccggctgc gcccgagagg ccctcgacga
     1321 gcacgagcac ctcgtagtcg gtcagatcgt tatcgagatc gaattccatg ccccacgacg
     1381 aagtgcgcac cgcgccgccg ggagctggat cgtcctcgag ccgcatccgc agatagagat
     1441 acgtcccgtc cgatgcgcgc aggcccgcgg gcgcggcgac atcaccgacg acgtcgcgct
     1501 cgtcgcgatt cgcgttgtcg tcgccgaacc cgtcggtcat caccgcgttg ccacaacgca
     1561 gcgggcgata ggcggtgtcc gcaggaaacg tttgcgcatg cgccacgagc ggcgccgcga
     1621 ggagtagcag cacgatgatg atcttcattt gctctcctgg atgaagaatt cgacgcggcg
     1681 gttcttctgc cggccaccgg cggacttgtt cggtgcaatc ggccgggtct caccattgcc
     1741 cttcgtgacg aggcggtcgc cgtcgacgcc acgcttcgag agctcgtcct tgaccgacgc
     1801 cgcgcggtct tcggacagct tctggttgag cggtggcggg ccgacgttgt ccgtgttccc
     1861 ctcgatgcgc accttcttga tctgcggatg cgcatcgagc gcctgcgcga tctgatcgag
     1921 cagcggtccg ctgcgcttgt ggacgtgcgc cgatccggtc tcgaactgga tcttgccgat
     1981 gatgatcatc tggccatcct cgatcttcac gtaggtgtgc gagggacaac catcgcgatc
     2041 cttctcgccc ttgagctcgg gacactcgtc gtcgccgtcg agcacgccgt cgccgtcgtt
     2101 atcgggctcg ggcttgccgt cgtcgtcctg atagccgtcc tcgtcctcgg gctcgtacgg
     2161 cgccttgtcg tccttgtcga gcacgccgtc gccgtcgctg tccttgaagc cctggcgatc
     2221 gggatggttc ttgcgatacc gcacgccgcc gatgatgcgg aagtccggat cgccgatgcc
     2281 gtcggtgagg ccgaacgagg caccgccgta cagcgcgaga ccacccattc gatactcgac
     2341 accgccgagc acttcgatcg ggaagtcggc gccgtcgacc tcggtgttga cgtgcttttc
     2401 ggcgaacatc tcggcgcgta gcgagagctc gtcggtgaag ttgtacgaga cccacgggcc
     2461 gagcgtgatc gaatcgccgt aggggagctg cggatgctcc gcgcgccagc ggtagccgac
     2521 gtcgagcccg agcccgagct gattcaggtg agcagcgaac aacagctcgg gggcgacagt
     2581 gaaaccaccc gcgccgcgga attcctcgtc gttgccggtc gggaacgtga tcggcaacgc
     2641 gaacccgagg agcacgtgct tgtcgagcga gccgctgcgg atgatcgcga ccttcggcac
     2701 gaagtacagg tcgccgacgc cgggacccgc ggcgagcccg ccatacggat cgccgtcgta
     2761 gatgaggtgg atcggcaggc cgaggccgag ctcgaggcgg ctgaagaggc cgatcgatcc
     2821 gacgacatcg gcggtcagcg cgcgatggac cggcgtcgac agaatcgcgc cggtcacgtc
     2881 atcgcgctcg acgacctgat cgtcggcgta gttgaggaac agccctaggc tccagccaag
     2941 gtgaaacggc acggctgggt gctcgttgac aaagccacct tccgcgccgg tggctggtct
     3001 aaatcgttcg gcatcgagcc cctgccccgc cgcggtccca cacatggcca ctagcagcgt
     3061 cgctgcaagt ccccgcatcg gacgatcgta acgtcactcg cgcgcgagat gccaccgcgc
     3121 gaccgcacgg aaactgcgtg cgagcgcaca ccacatcagt aagcgcgaaa acatggatct
     3181 cgcgcgatga tgatctcgct cgctgccctc gtcgtcaggg ctcgaggacg accttgccaa
     3241 atgccttgcg gtcttcgagg agacgatgtg cgtccgccgc gcgcgcgagc ggcaacacct
     3301 cgtgcacgac cggattcaac ctgccggccg cgacatgcgc gagcaccgcg agcagatcgg
     3361 ccttgctgcc catcgtcgag ccgagcacct cgacctggcg aaagaaaatg tggcgaagat
     3421 cgatcgccgg gtgaaagccc gcggtggcgc cgcacgtcac gacgcgaccg ccgttcctca
     3481 cggccttgat gctgccgacg aacacgtcgc cgccgacgtg ctcgatcacc gcgtcgacgc
     3541 cgcgcttatc cgtcagcttc ttgcactccg cgatgaagtc ggtcttcgtg tagtcgatcg
     3601 tgatgtccgc gccgagcgca cgagcgcgct cgagcttgtc gtcggtgctc gcggtcgtga
     3661 ttacccgcgc gccatggagc ttcgcgatct ggatcgcggc aacgccgatg cccgagccgg
     3721 cgccttgcac gagcacggtg tcgcctggct ggacgcgggc cttgtgcacg accatctgcc
     3781 acgcggtgag aaacggcagg atcgacgccg ccgcctgcgg gaacgtcagc cgctcgggat
     3841 acggcgcgat gttgacctgc gggacgacga tgtactcggc gtagccaccc tgcgtgttct
     3901 cgccgaggat cttgtagaag cggcacaggt tgtcgtggcc gccgaggcac gcggcgcact
     3961 tgccgcacga caggccgggc tgaacgacga cgttcgctcc gacatcacct ttcgcgcccg
     4021 ggccaagcgc gtcgatcgtg ccggcgatgt cgctgccgag gcgatgcgga tattcgagct
     4081 tgaacgcagg tccgcctttg cggacccaga tgtcgagatg attgagcgcg acggcgcgaa
     4141 tccgcacgcg cacctcacct ggaccgggct ccggaaccgg gagttgttcg aggtggagca
     4201 catcgggccc gccgtgctcg cgcaacacca tcgcggtcat cgtcatgggc ggggatataa
     4261 cgtagattcc tcgcatgcgc gtcgccgcga tcccctgtct cagtgacaac tacgcctacc
     4321 ttgtcatcga cggcacgcag gccgcgattg tcgatccgag tgaagcaccg ccagtgctcg
     4381 cagcgatcga acgcctgggc gtcacgccgg cggcgatctg gctgacgcat catcatcacg
     4441 atcacgtcgg cggcatcgaa ggcatcgtcg cgaagtatcc gaacgtcgag gttgtcggcc
     4501 acaccagcga caaggcacgc atccccaagg tgacgaagct cgtcaacgaa ggcgatgtcg
     4561 tcacgctcgg ctcgctgaag gcgcacatca tccacaatcc gggtcacacg ctcggcgcga
     4621 tcagctactg gatcaaggac ggcgatggcg cggtattcac cggcgatacg ctgttcggcg
     4681 ccggttgtgg ccgcatcttc gagggcacgc cgccgatgat gcacgagtcg ctgcacaagc
     4741 tcgccgcgct accaccgacg acgcacgtct acttcggtca cgagtacacc gctgcgaacc
     4801 tcaagttcgc cgtttacgtc gagccgaata acacggcggt cgccgcgcgg ctcgagacgg
     4861 tcacgacgcc atcgacgccg tcgacgatcg cgagcgagcg cgaaacgaat ccgttcctgc
     4921 tcgcgaagtc cgtcgacgag ttcgcggcgc gccgcgaagc gaagaactcg ttcaagtagc
     4981 cgcaaacatt tcgcgccaat cgcgcgccat atcgacgacg acccagttgc ggtcgggtgc
     5041 cgcatcgacc gcacgcgaca gccggccgaa gtgcgtgtcg cggtcgtacg cgaattcgcg
     5101 cgcggcgtcg gtgtgatgca cgagcacgcc gaggcgcgga ccgtcgcccg ccgtcgtcca
     5161 gtcgagcatc tcgaaatcgg cgtcggagtt gccgaacgcg aggatcgggc gccggccgat
     5221 cgcctcgtag atcgcggccg gtttggcggc acgatcgtcg acgggctcga tctcaggaag
     5281 ccgatacagc acggggcgat tgccgcggta ctcgtagtgc agcttgacgc ggctaccgat
     5341 cacctgctcg ggcggcacgc catacacgcg ttgcgtccac ggccgtatga gctccacgcc
     5401 gccgccggac acgatgaacg tggtgaagcc gttcgcgcgc agataagcga gcatgtccac
     5461 catcggctga tacacgagct cggtgtacag ccgccgcaac gtcggatggc gtgcgttcat
     5521 cagccagccg atcacgatct cggtgaactc gtcggtcgta tcgttcgcgt gcgttgcagc
     5581 gactagctcc gccgcaccgc gctcgccggt tgccgccaac gctgcgaggt tgtcctcgag
     5641 cgccgccttg aatggctgct gcgcgtgcca ctcgggatac gccggcgcca gtgccttcac
     5701 gcgatcgatc gcgaacgcga gctgcgtgta catcggctgc tcgacccaca tcgtcccgtc
     5761 gtggtcgaac acggcgatgc gctcctgcgg tgggacgaag tcggcatgcc gatcgcgggt
     5821 gacccgcgtg acaaattcga cgatggcttg cttcgcgccg cggtcatgcc acgacggcaa
     5881 cggatccgag acgaccacgt cagcgtcctt ccgtcatcga ggcttccatc gcctgcatcg
     5941 cctgatcgat cgtgaacgac gcggccttct ggcgcggtgg gaactctttg aacgtctcga
     6001 ggaacttcgt gacgatcgac tgtgccgcca tgatcatgta cgccttactg aggtaccagt
     6061 cccagtaggt gttcgacgtg acgtccgcgc gctcgaacgg atctgtgcgc aggttgaaca
     6121 gcttcgggat gcgcagcttc gtgaacggtt cgccccagac ttcgagcgtg ccccggcggc
     6181 gctgctcgag gaacacggct ttccagttac cgtagcgcag cgcgacgaga tcaccgtcgt
     6241 cgctgaagta gatgaagccc ggccgggacg atgtcttgtc cttgcccgtc agatagccga
     6301 gcaggttata gccgtcgata tggttcttga acgtcttgcc caccgcgctg tagccgctgc
     6361 gtagcttctc catgatgtgc ggctcgcccg ccgcggcgag gaacgtcggc agccagtcgt
     6421 gatggctcac gatctcgttc gagatcgagc ccgccgcgat cttgcccggc cagcgcaaca
     6481 cgcctggcac gcggaacgcg ccttcccagt tcgtgttctt ctcgctgcgg aacggcgtca
     6541 tcgcaccgtc gggccagctg ttcatgtgcg gcccgttatc ggtcgtgtag agcacgatcg
     6601 tctcgctcgc gatgccgagc ttgtcgagct ggtcgagcat cgagccaatc tgcttgtcgt
     6661 gatcgatcat cgtgtcgtga tacggcgact gactgcgacc cgcctggccg acgctgctcg
     6721 gcttgggatg cgtgcggaaa tgcatatgcg tcgtgttgag ccagacgaac cacggtttat
     6781 tcgccttggt ctggcgctgg atgaagtcct gcgccgcggc aacgaattcg tcgtcgcacg
     6841 tctccatccg cttgcggttg agcggcccgg tatcctcgat cttctgcttg ccgaccttgc
     6901 cccagcgctt gtcggtcgtg gtgtcgtcct tgtcggtcgc ccacgatttg atcacgccgc
     6961 gcgggaggaa cttcgcgcgg aactcggggt tcgtcgggta atcggggtct tcgggatcct
     7021 cctcggcgtt caggtgatag aggttgccga agaactcgtc gaagccgtgc acggtcggca
     7081 agtactcgtt gcggtcaccg aagtgattct tgccgaactg gccggtcgcg tagccgagcg
     7141 gcttgagcag ctcggcaatc gtcacgtcct cggcgtgcaa accgaccggc gcgccaggca
     7201 tgccgacctt cgacaggccc gtgcgataca ccgactgccc ggtcatgaac gatgcgcgtc
     7261 ccgcggtgca gctctgctcg ccgtacagat cggtgaaccg catgccctcg cgagcgatgc
     7321 gatcgatgtt cggcgtctga tagcccatca tgccgctcga gtaacaggac aggttccaga
     7381 taccgatgtc gtcaccgaag atgacgagga tgttgggctt cttcgctgcg ttcgagttcg
     7441 ccattgccgc tgcctccagc cggcacgcga agcaagcggc gttcctcgcg cagctgtgtc
     7501 cgatcctgta gtcaccggcc acgtattgcg cagccgcgca tcgcagtggc gtgctttacg
     7561 tagcgcgaac gtgcgcacat gcgccgatgc gcatcacttc gcgacgaaga tcgcgacggc
     7621 cctcgcgccg agatcgtaga gcgactggtt catccggtac tcctcgccgg gagcatggaa
     7681 gttgttttgc gcctccatga acgcggacgt atcgccggcg cgataccaag cgagcccgct
     7741 cggcggcgcc ggcaacgtca cgcgaacgac ctgcgcgctg tggttgtaga cgatgtacag
     7801 cgtctgatcg ccgaacgatg ggccatcgag gcgccacgcg agcgccggct tcgtcgcatc
     7861 gtccatgtag ccgccgctga cgacctggcc agccgcatcg cgccacgaca cctgcgtcgg
     7921 ctcgatccat ttctcggggt gcagtgcggg atgcgcggcg cggaactgta acagaga
//
LOCUS       DASUFX010000679         5231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2054600, whole genome shotgun sequence.
ACCESSION   DASUFX010000679 DASUFX010000000
VERSION     DASUFX010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054600"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFV99_27405"
     CDS             <1..324
                     /locus_tag="VFV99_27405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5063145.1"
                     /translation="VDIAKNLKPSARNELEITDVNKEYLRRGKLRACVLSRGNAWLDT
                     GTHDSLLEAANFVSVVEKRQGLKVSCIEEIAYRLGYIDAAAVRSIAASLKGSEYARYL
                     LDMLA"
     gene            321..878
                     /gene="rfbC"
                     /locus_tag="VFV99_27410"
     CDS             321..878
                     /gene="rfbC"
                     /locus_tag="VFV99_27410"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX5063146.1"
                     /translation="MKTLPTTLPGVVLLELDVYGDHRGRFMETFRRDRYREIGVALDL
                     EFVQDNFSSSAKGILRGLHHQLLHPQGKLVLCTRGEIWDVCVDVRRGSPTFGQWFGCT
                     LSADNHRQIWIPPGFAHGFVVVSEQADVTYKVTAVYAPADEKSIRWNDPDLAIEWPLH
                     DEPTLSARDKGAPLLRDSELPVYTP"
     gene            875..1729
                     /gene="rfbD"
                     /locus_tag="VFV99_27415"
     CDS             875..1729
                     /gene="rfbD"
                     /locus_tag="VFV99_27415"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5063147.1"
                     /translation="MKILVTGGKGQLGRAVARRGAALGHVIAAPDIDELDISDEQQVH
                     RALRDFAPAIVINAAAYTAVDKAESERGRAYAINRDGAGTVARVCAAARTRLLHVSTD
                     YVFDGTATRPYREDDPLAPLGVYGESKAEGEHAVHEIGGTIVRTSWLFEGKGPSFVHA
                     ILRLAKERSVLRVVADQRGCPTWADDLADALFVLAERRTLDAVYHFCNAGETTWHAFA
                     VAIVDEARKYRTLACERIDPITTAEYPTAAKRPAYSVLDTSRIRALGIEPPSWQSGLA
                     AVVEQELS"
     gene            1731..2765
                     /gene="rfbB"
                     /locus_tag="VFV99_27420"
     CDS             1731..2765
                     /gene="rfbB"
                     /locus_tag="VFV99_27420"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX5063148.1"
                     /translation="MRRIVVTGGAGFIGFNFVRELLAAEPQTEVVVLDKLTYAGNPRS
                     LAELSGQSRFKFVRGDIGDKTLVRELLGSIKPAAIVHFAAESHVDRSIDGPEDFIQTN
                     VVGTFHMLEETRRFLGENPDPAFRFLHVSTDEVFGALGPTGSFLETTPYDPRSPYSAS
                     KAASDHLVNAYVHTYKFPAITTNCSNNYGPYQFPEKLIPLTIANALAGKRLPVYGKGE
                     NVRDWIYVTDHCEAIRIVLERGRIGECYLVGSRNEQKNLDVVHTICDVLDELHPAGKP
                     HRSLIEFVTDRPGHDFRYSIDPTKIESKLGWRASVNFAVGIRKTIEWYLAHREWTDAI
                     RSGTYREGIS"
     gene            2762..3463
                     /locus_tag="VFV99_27425"
     CDS             2762..3463
                     /locus_tag="VFV99_27425"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5063149.1"
                     /translation="MIGPLRGVVRRVLGQLPESVAESASFELRSKLGRSRARRLTIPT
                     GEKVYINVGSGGDSFSGFIGIDFFGDPTAYGADLRYPLLVDDDTVDGIFTEHTLEHLT
                     YDEDARLLQECFRILKPGGRIRVVLPDVSLFAKHYAAGDTAWFAQWEELVLAPRGRKM
                     VSPMTAISFVTQEFGHRSAWDMTTVERFLTAAGFTDVIACRYGSGADPILVKDNSSPD
                     RTFLSLYAEAVKPVR"
     gene            3509..4381
                     /locus_tag="VFV99_27430"
     CDS             3509..4381
                     /locus_tag="VFV99_27430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063150.1"
                     /translation="MKMYDALRLAAGWVLPPNVERALTRSAERVFTRSLAANKALRNR
                     HADKQRCFVIGSGPSLKTQDLSRLANEYTIAANSFYKHPDAGKVGLNYLCIGDPHFMS
                     AEPRALDWHRTIEKALPSTTMVLNRVAQPIVEEHGLYPNQEVFYVEGSMVPATKASEC
                     ALDFTRRLNVGTSTGTLVMIPLAIYLGFKEIFLLGFDANWLENLEKSYHFYDSHEHFP
                     EFDSVAADTRGFSYEGELDTVSREFKAHRLLAERSRQLGVKIWNATAGGRVDTYPRVR
                     YEDVIARSRTASSM"
     gene            4441..>5231
                     /locus_tag="VFV99_27435"
     CDS             4441..>5231
                     /locus_tag="VFV99_27435"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX5063151.1"
                     /translation="MGANAGWLLADKALRFPVALGVSIAVARYLGPERQGVLGYAIAF
                     AGLFSVIAGLGLDGITVRELLATPDERDRVLGTSFLLKLLGGIGALVLAIAVIAIVRP
                     GDTTMLVYVAIIAAATLFQAFDVTDYYFQSQVQSKYTVFAQAPVFLALSLIKIVLIAL
                     NAPLTAFVWISFADLAFASLSVALVFRARVEPLTRWRFAKERGKAMLRDSWPLALSAA
                     VGMVYLRIDQVMLGQMLGDEEVGVYSVAVRLAEIVMMASMVIYSST"
BASE COUNT          908 a         1678 c         1660 g          985 t
ORIGIN      
        1 gtcgatatcg ccaagaacct gaagccgtcc gcgcgcaacg agctcgagat caccgatgtc
       61 aacaaggagt acctgcgccg cggcaagcta cgcgcgtgcg tgctctcccg cggcaacgcg
      121 tggctcgaca ccggcaccca cgactcgcta ctcgaagccg cgaacttcgt ctcggtcgtc
      181 gagaagcggc agggcctcaa ggtcagctgc atcgaggaga tcgcgtatcg gctcggctac
      241 atcgatgcgg cagccgtgcg ctcgatcgcg gcctcgctca agggcagcga gtacgcgcgc
      301 tatctgctcg acatgctcgc atgaagacgc tgccgaccac gttgcctggc gtcgtgctgc
      361 tcgagctcga cgtgtacggt gatcatcgcg gtcggttcat ggaaacgttc cgtcgcgatc
      421 gctatcgcga gatcggcgtc gcactcgatc tcgagttcgt tcaggacaac ttctcgtcgt
      481 cggcaaaggg aatcctgcgc ggattgcacc atcagctgct tcatccgcaa ggcaagctcg
      541 tgctctgcac gcgtggcgag atctgggatg tgtgcgtcga cgtccggcgg ggctcgccga
      601 cgtttggtca atggtttggt tgcacgctct cggccgacaa ccaccgtcaa atctggatcc
      661 cgcccggctt cgcacacggt tttgtcgtgg tctcggaaca ggccgacgtc acgtacaagg
      721 tcacggctgt ctatgcaccg gcggacgaga agtcgatccg ctggaacgat cctgatctcg
      781 cgatcgagtg gccgcttcac gacgaaccga cgctgtccgc gcgtgacaag ggcgcaccgc
      841 tcctgcgtga ctccgagctt cctgtctaca cgccatgaag atcctcgtca caggtggcaa
      901 aggccagctc ggtcgtgcag tcgcacgccg tggtgccgcg ctcggacacg ttatcgctgc
      961 gccggatatc gacgagctcg acatcagtga cgagcagcaa gtccaccgcg cgctgcgcga
     1021 cttcgcaccg gccatcgtga tcaacgcggc ggcatatacc gctgtcgaca aggccgagag
     1081 cgaacgcggg cgcgcctatg cgatcaaccg cgatggcgct gggaccgttg cacgcgtctg
     1141 cgcggcggcg cgcacgcgct tgctccatgt ctcgaccgac tacgtgttcg atggcaccgc
     1201 gacgcgcccg tatcgcgagg acgatccgct agcgccactc ggtgtctacg gcgagagcaa
     1261 ggccgagggt gaacatgccg tgcacgaaat cggcggcacc atcgttcgta cctcgtggct
     1321 attcgagggc aagggaccga gcttcgtcca cgcgatcttg cggcttgcga aggaacgctc
     1381 ggtgctgcgc gtcgtcgccg accagcgcgg ctgcccgaca tgggcggacg acctcgcgga
     1441 tgcgctgttc gtgcttgccg aacgacgcac gctcgacgcc gtgtaccact tctgcaacgc
     1501 gggcgagact acgtggcacg cgttcgccgt cgcgatcgtc gacgaggcgc gcaagtaccg
     1561 cacgctagca tgcgagcgca tcgatcccat cacgaccgcc gagtacccga cggcggccaa
     1621 gcgccccgcg tactccgtgc tcgatacatc gcgcattcgc gccctcggaa tcgagccgcc
     1681 gtcgtggcag tctgggctcg ccgctgtcgt cgaacaggag ctgagctgat atgcgtcgta
     1741 tcgtcgttac tggtggcgcc ggtttcatcg gcttcaactt cgtgcgcgag ctgctcgctg
     1801 ccgagccgca gaccgaagtt gtggtgctcg acaagctcac gtatgccggg aatccacggt
     1861 cgctcgcgga actgagcggc caatcgcggt tcaagttcgt gcgcggcgac attggcgaca
     1921 agaccctcgt gcgcgagctc ctcggttcta taaagccggc cgcgatcgtc cacttcgcgg
     1981 cggagagtca cgtcgaccgt tcgatcgatg gtcccgaaga tttcatccag acgaacgtcg
     2041 tcggcacgtt ccacatgctc gaggagacgc gccgctttct cggcgagaat ccggatcccg
     2101 cattccgctt tcttcacgtc tcgaccgacg aggtattcgg cgcgctcggt ccgacgggct
     2161 cgttcttgga gaccacgccg tacgatccgc ggtcgccgta ctcggcatcg aaggcggcga
     2221 gcgatcacct cgtcaacgca tacgttcaca cgtacaaatt cccggcgatc accacgaact
     2281 gctcgaacaa ctacggtccg taccagttcc ccgagaagct gatcccgctg acgatcgcga
     2341 acgcgctcgc cggcaagcgc ctgcccgtct acggcaaggg cgagaacgtg cgcgattgga
     2401 tctacgtgac ggatcactgc gaggccatac gcatcgtgct cgagcgtggc cggatcggcg
     2461 agtgctatct cgtcggctcg cgcaacgagc agaagaacct cgacgtcgtg catacgatct
     2521 gcgacgtgct cgacgagctc catccggcgg ggaagccgca tcgctcgctg atcgagttcg
     2581 ttaccgaccg ccccggccat gacttccgct actcgatcga tccgacgaag atcgagagca
     2641 agcttggttg gcgcgcctcg gtgaactttg cggtcggcat ccgcaagacg atcgagtggt
     2701 atctcgcgca tcgcgagtgg accgatgcca tccgctcggg cacgtaccgc gagggcatct
     2761 cgtgatcggt cctcttcgcg gcgtggttcg acgcgtgctc ggccagcttc cagagtcggt
     2821 cgctgaaagc gcatcgttcg agctgcgatc caagctcggc cgcagccgcg cccgccggct
     2881 caccattccc accggcgaga aggtctacat caacgtcggc agcggcggcg attcgttctc
     2941 cggcttcatc gggatcgact tctttggcga cccgaccgcg tacggcgccg acctgcgcta
     3001 cccgttgctc gtcgacgacg ataccgtcga tggcatcttc accgagcaca cgctcgagca
     3061 cctgacctac gacgaagatg ctcggctgct gcaggagtgc tttcgcatcc tcaagccggg
     3121 aggtcgcatt cgcgtcgtgc ttccggacgt atcgctgttc gcaaagcact acgcagccgg
     3181 cgacacggcg tggttcgcgc agtgggaaga actagtgctc gcgccgcgcg gccgcaagat
     3241 ggtctcgccg atgaccgcga tcagcttcgt gacccaggaa ttcggccacc ggtcggcgtg
     3301 ggacatgacg accgtcgagc gctttctgac ggcggcgggc ttcaccgatg tcatcgcgtg
     3361 ccgctacggc agcggagccg acccgatact ggtgaaggac aattcctcgc cggatcgcac
     3421 gttcttgtcg ttgtatgccg aggctgtcaa gccggtccgg tagcgcttct tgacagtccc
     3481 cgcagtcgcg cgttctagtg agcggcacgt gaagatgtac gacgccttgc gacttgcggc
     3541 ggggtgggtg ttgccaccca atgtcgagcg tgcgctcacg cgttcggccg agcgcgtgtt
     3601 caccaggtcg ctagcggcga acaaagcgct gcggaaccgg cacgccgaca agcaacgctg
     3661 cttcgtgatc ggcagcggac cgtcgctgaa gacgcaagat ctgtcacggc tcgcgaacga
     3721 atatacgatc gctgcgaaca gcttctacaa gcaccccgat gctgggaagg tcggcctcaa
     3781 ctacctgtgc atcggcgacc cgcacttcat gagtgcggag ccgcgcgcgc tcgactggca
     3841 ccgcacgatc gaaaaggcgt tgccgagcac gacgatggtg ctcaatcgag tcgcgcagcc
     3901 gatcgtcgaa gagcacggct tgtatcccaa tcaggaagtg ttctacgtcg agggatcgat
     3961 ggtgcccgcg acgaaggcga gcgagtgcgc actcgatttc acccggcggc tcaatgtcgg
     4021 aacgtcgacg ggaacactcg tgatgatccc gctcgcgatc tatctcggct tcaaggagat
     4081 cttcctgctc gggttcgatg cgaactggct cgagaatttg gagaagagct atcacttcta
     4141 cgattcgcac gagcactttc ccgagttcga ctcggtagcc gcggatacgc gcggcttttc
     4201 gtacgagggt gagctcgata ccgtgagccg agagttcaag gcgcaccggc tgctcgccga
     4261 gcgctcacgg caactcggcg tgaagatctg gaacgcgact gccggtggcc gcgtcgacac
     4321 gtatccgcgg gttcgctacg aggacgtgat cgcacgttcg aggaccgcga gctcgatgtg
     4381 aagtacgcga cacgcgcgta ctggcgccag tggctgagcg atcggccaaa tctgcacaag
     4441 atgggagcca acgccggctg gctcctcgcg gacaaggcgc tgcgatttcc ggtcgcgctt
     4501 ggcgtcagca tcgcggtcgc gcgttatctc gggccagagc gccaaggcgt gctcggctac
     4561 gcaattgcat ttgccgggct gttctcggtg atcgccgggc tcggcctcga cggcatcacg
     4621 gtgcgcgagc tgctcgcgac acccgacgaa cgcgatcgcg tgctcggcac gtcgttcctg
     4681 ctcaagctgc tcggcggcat cggcgcgctc gtgctcgcga ttgctgttat cgcgattgtc
     4741 cgtccgggcg atacgacgat gctggtctac gtcgcgatca ttgccgcggc gacgctgttc
     4801 caggcattcg acgtcaccga ctactacttc cagtcacagg tgcagtctaa gtacacggtg
     4861 ttcgcgcagg caccggtgtt cctggcgctc tcgctgatca agatcgtact gatcgcgttg
     4921 aatgcgccgt tgacggcgtt cgtttggatt tcgtttgcgg acctcgcgtt cgcgtcactg
     4981 tcggtggcgc ttgtcttccg cgcgcgtgtc gaaccgctca cgcgctggcg gttcgcgaag
     5041 gagcgcggca aggcgatgct gcgcgacagc tggccgctcg cgctgtcggc ggccgtgggc
     5101 atggtgtacc tgcgaatcga tcaggtgatg ctcggccaga tgctcggcga cgaggaggtc
     5161 ggcgtgtact cggttgcggt tcgcctcgcc gagattgtga tgatggcatc gatggtcatc
     5221 tactcgtcga c
//
LOCUS       DASUFX010000680        12176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1776881, whole genome shotgun sequence.
ACCESSION   DASUFX010000680 DASUFX010000000
VERSION     DASUFX010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776881"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFV99_27440"
     CDS             <1..1130
                     /locus_tag="VFV99_27440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063152.1"
                     /translation="TSPITDSNYSIELYDGVALGNTAVVGMGGATVALAIGTAGTLFN
                     PSPPAVKPTTDTDTWSWDYHLDYLNGSLSTDYDNNGFTSEESGTSVFTGGLGLRIGEW
                     AGAITATQQYAPVADSAVMVGTNMVPLQASVLRLQIALARWVSRLDTAIGISAQSAIF
                     ELKPDCAGAGCEALFDVNGGGLELGATWAPRRQSLRIGAALATPIAGGNVSGCDPMDC
                     NGFILPEKIVTPWRLAAGGAYRFSASDWNQLVGGYFRDERSVTVAADVIVTGSSDNAF
                     GLERFGQHELQRSGRHASFGVRGGVEYEWLPGRLRLRSGSYWEPGRFVGVGGRLHVTF
                     GIEVRVFQFWFWGTYRRGRISLTADAAERYRNGGLSVGFWH"
     gene            complement(1150..2274)
                     /locus_tag="VFV99_27445"
     CDS             complement(1150..2274)
                     /locus_tag="VFV99_27445"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5063153.1"
                     /translation="MEPTTTLRLADKLRAQARAGNCATAEDAAPFTVSALRYRDAARC
                     EREQALFGGPRIVAVSASLVSGACLPVDLPGRAALVVRGTDGVVRAFANACRHRGTRL
                     VDKPCAAKALTCPYHAWTYDLTGKLVHVPHADSFPGIDLDTRALAPLPVTERDGLVWL
                     GASDTDDLDCYLGPLGADVAALGLNQHVLWERSTTTRRCNWKLVVEAFLDGYHIRVLH
                     RDSVYRFFLDAASLAEPVGPHIRAVTGRRALLEAPSDLASAGDLRQLATPSLLLFPST
                     VFVEHPDFLSIMTVHPLAPDVTAWEHLMLVPAARAHETEHWNKSWQLIEEGVFQREDL
                     WVCEQAQRSIDAGAVDELLFGSLESPARWFHAAIDAAMRA"
     gene            2309..2752
                     /locus_tag="VFV99_27450"
     CDS             2309..2752
                     /locus_tag="VFV99_27450"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5063154.1"
                     /translation="MQDDRLRSSLANAVVRLFRQVNRVHNRLFADHDVSAEQAHILLI
                     LGVYGPMTIGRLQKQLSLSSATLTGAIDRLEAQELVRRVPSPRDGRAFVIESRLPDRK
                     RAQLEAIVEKGDQRCFSALNAGERKELLRLLEKCAAQLEETAAAK"
     gene            complement(2697..4355)
                     /locus_tag="VFV99_27455"
     CDS             complement(2697..4355)
                     /locus_tag="VFV99_27455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5063155.1"
                     /translation="MRRLLIVVMVTVAPGVAMAQTWQDATANCIGTTAEWTNKVEVAD
                     VDGDGKVDLLLANGGDYSSPGNPPNNEPTRIFKNTWGGTTNCTEISATALGGFTGMSR
                     MIKAADIDGDGDLDLLTGGAYQTQLKLYKRDAASWTDATAQLPQQLTSIGDAEFGDVD
                     NDGDLDIVLADWGGTNPGTNTGGRTRLYLNNAGTFTEATATQMPDLLVKWSWDIELAD
                     VDNDWDLDVLVSCKRCTTSYVFKNDGTGHFTDDPNALPHFTNNYEFEPMDIDKDGDLD
                     LITINDGPQVTEHIFVNRGDGTFADETSMRLTGTANPANADDNVAVWLDYDDDGDPDL
                     LIGTLGADRLLSNDGSGKFTLVANATPNDTNATLGLAVADFDGDGRIDILQGQGEVAF
                     PDKVQLASMAVKVDTTPPIVRVATSLSGNILTARVNDLVGPSHVHDFQKVVVVVGSTE
                     IPMQWYGEMLWRAAIPTEAMSATYQVCATDRQGNKGCATNAPTGDADAGVGDDGGTKP
                     PGGGGGGCCEASGDQRGALVLIALVAITWRRRSPRVARRTSRAA"
     gene            4537..6015
                     /locus_tag="VFV99_27460"
     CDS             4537..6015
                     /locus_tag="VFV99_27460"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5063156.1"
                     /translation="MGGASAAAIAGPYFMRRAHAQFGAFPGGAEAATLPEGLRAKRVL
                     EVFLYGGLSQWETLYFVRNYGTPTDATYPNQQYYTYASSNATMLPRCGTASFDTVRDF
                     GQDALGANVEIGPFADRLWGRQDVVDRMRIVVQKHTLEPHEAAVPQALTGRPVGQPTA
                     AGLGAHVQRARLDGGVTPDRASPYSYVFATGGISSDNVAAAAAPGVHPGAARPLLIKT
                     DNATTFTSLLGRSTVGGARQNHDALISAYTNQYNARLTWPNGTRVRSARTDDFEVAFN
                     NAKRVDAISAVIAQDLFNTQGGTSCQTTKSRDIPLIGLSAAARLLTHPTEPASYVCVS
                     DTGLYEASGGGGYDTHTDNARDTATNFDNMLKSLLGIINAPGETDPKKISLDDTLIIL
                     NTEFGRTPRAQGTTGRNHHPYGYVTAFIGGPTTKGVSGAIGPNGMADMYATPAQNRIA
                     ALLALGIWPFSQEAFAVSDVPGATGEVDAALKAMQMCLGRTA"
     gene            6012..8072
                     /locus_tag="VFV99_27465"
     CDS             6012..8072
                     /locus_tag="VFV99_27465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063157.1"
                     /translation="MKRLAVIAIVLVACGTDHRKPDDGDDTQDVPYTECGGDPASFVR
                     QALVSLDGHRPRSQAEVDVYVDLYNAVKTAGGDPKETVSRAIMERPEFADRWIDTFMD
                     AMHVQRIDVQNEASCWDKAVRTTVDAGLATAIRMQAATTSGDGKGAWTMADLARSAMV
                     LDDPTPLYRAQIFSMVAHPIPAANVPAKEAELARRADFGETFDSGFLHRDVVCLACHN
                     SERSVTDSDDPVTDRHWPVPGMPEKAVYGTSAGIEMDRAHAAFRVDGFVGGNSHPWGW
                     DADCGGFSTTVGDDIAGVDGKLASLTGKRITVYGLEQALARGFDKLRGQLPPIGMDGA
                     IADPDAALAWLVTLKITEDVWKRATGTSLTIANYFPRNQASSDLLYSLATKFTQSGYS
                     VKALLVAIVQSDYYNRQPPELGCGKEPYTYPAVFDPWVTSDADPERRFNGPGDAVTAL
                     DARTLVTATNAALEWPAPPAASRFPDYGDGCENDTCSQMQSSCNQFGGCCNSYKAACM
                     MGGVMPSIEVPFERGVGMFLRNSERGFRGLDFQARLVWEDRTGACARPTWASSDFIDK
                     IVAAGAADTTATVGDVAAAIKDRLIGESAIVDAQEIAAITGLLGAAPEMPASNVTVAA
                     ARQFCGALLGSPQFLLQGIAGRGGDRPKLTPTDVGYDAICADVATHVAGKTVSCTGGK
                     LTVQ"
     gene            8182..8895
                     /locus_tag="VFV99_27470"
     CDS             8182..8895
                     /locus_tag="VFV99_27470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5063158.1"
                     /translation="MACENKSKPKDTGEAAQKPAGNETPTPVNKEPELPKQPSPDPTP
                     AADVRPPVAADLAEYTKDIPGTGTKLMAKIETSMGTFNCELYGDKAPMTVANFVGLAT
                     GKKPWLSPSGMQKDKPFFDGLTFHRVIPGFMIQGGDPRGDGTGGPGYKFNDEIWQGQH
                     IEPGVLAMANAGVRGGEGTNGSQFFIMEGARPDLDTHHTVFGKCKELDLVKKIENVPS
                     TDDKPNTPVTINKITFSKA"
     gene            8939..11182
                     /locus_tag="VFV99_27475"
     CDS             8939..11182
                     /locus_tag="VFV99_27475"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063159.1"
                     /translation="MVASARFLASFLAASLITGCGAARPARPMQLQRVILYQNGIGYF
                     ERAGHVQGETLRLSFSRGELDDVLKTLTVIDRLGAGVATVDVPTLDDKATSIGLGVRM
                     SAGRVHDVKVSYAVPTPTWKAAYRVVLDDKTKAQSLIQGWAMVNNASQEDWRDVQLTL
                     ATGAPMSFVHDLHTPEYVKRPDIHGNLVAPIVTGPIDSEKVGAGDSDSDGIPDVDDVC
                     PDSPEDRDSFEDTDGCPDADNDRDRVNDAYDKCPNEPETWNGFEDDDGCPDRGRVVVT
                     SSAIEVLEQIYFAKTSDGIKKESGEILDAIAATLRGNPDIVKVEVGGHASDDEGEAWA
                     LSSRRASVVRQALIQRGVEGKRLVVVPYGSTRPLDPKAADKNRRVDFLIAQRQEEMHA
                     MPTRRAPTRIDTQTAAASVHTATKPAEVAGTVRYELTEPVSIRKGSSSMVSILNKSVS
                     GEDVYLFRPDPNAPGSARHPFRAVRLVNDSGYMLEPGPIAIFARGTFVGDSMIGRLNV
                     GETAWIPYALDGATQVTAATDEAERPIKIVSIQRGVLTVENAGVRVTKYSIAAGADPA
                     KQIYLRHDKAYGFAAKDLPPGTQDRGDSYLIPLPLQAKKTSVLSIEERQPRRHTMHIL
                     DAGATEIGLYVEGSHLAPAIEAKLEEAIELRKEMGKIEEGLDGLRERIVDLSERADEI
                     RENIRALDKVRGADPLRKKLVASLTAVTTEADALARKLGGESEALATARNKLQDALRE
                     INLDEKT"
     gene            complement(11179..>12176)
                     /locus_tag="VFV99_27480"
     CDS             complement(11179..>12176)
                     /locus_tag="VFV99_27480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063160.1"
                     /translation="IEAGGIQNVLESERKAVYRRADGSVAGTQTMKTGQMHDQSTNPN
                     DKPGGPATPYVDRDNSKTGGSKGKGGPWYTDSVTLEPTDQFKDNVLLVKDKPSQTLNL
                     KVGDAVLDEIQGHEKFVTTAAVQRGGETAPLVSEQWSIPWSMKLDKEHKGEGAEVTHT
                     ASPDQHASVTDGKLPVNEKEWWSFADEAQALAAPRHMLLQSLKISKVKDPASYGFAMS
                     AIKKLNWSFEAQVNCASKYSWIEKDQMELSVITNKTSVTKAMSIGKGESHALSFRFLD
                     VYDPDSFNWDRPMIFMLTGEGQVLTFHCYAPFTEVTNANSGKDSGADHYSMKIFG"
BASE COUNT         2105 a         4073 c         4006 g         1992 t
ORIGIN      
        1 caacctctcc gatcactgac agcaattatt cgatcgagct atacgacggg gtcgcgctcg
       61 gcaacaccgc ggtcgtcggc atgggcggcg cgacggtcgc gctcgcgatc ggcacggcgg
      121 gcacgctgtt caacccgtcc ccgccggcgg tcaagccaac gacggatacc gacacgtgga
      181 gctgggacta tcacctggac tacctgaacg gctcgctctc gaccgactac gacaacaacg
      241 gattcacgag cgaagaaagc ggcacctccg ttttcacggg aggcctcggc ctgcgcattg
      301 gcgagtgggc cggcgcgatc actgccaccc agcagtacgc accggtcgca gactccgccg
      361 tcatggtcgg aacgaacatg gtgccgctgc aagcgtccgt gctgcgcctc cagatcgcac
      421 tcgcacgctg ggtcagccgg ctcgacaccg cgatcgggat ctcggcgcag agcgcgatct
      481 tcgagctcaa gccggattgt gcgggcgccg gttgcgaggc gctgttcgac gtcaacggcg
      541 gcggcctcga gctcggcgcg acctgggcgc cgcgccgtca gtcgctccgg atcggcgctg
      601 cgctcgcgac accgatcgcg ggaggcaacg tgagcggctg cgatccgatg gactgtaacg
      661 gcttcatcct gcccgagaag atcgtgacgc cctggcgact cgcggccggc ggcgcctacc
      721 ggttctcggc gtcggactgg aaccagctcg tcggcggcta cttccgcgac gagcgcagcg
      781 tcaccgtcgc ggcggacgtc atcgttaccg gctcgtcgga caacgcgttc ggtctcgaac
      841 gatttggcca gcacgagctg cagcgctcgg ggcgtcacgc gtcattcggc gtgcgcggtg
      901 gcgtcgagta cgaatggctg cccggccggc tgcgtctgcg cagcggcagc tactgggagc
      961 ccgggcgctt cgtcggcgtc ggtggccgtc tgcatgtcac gttcggcatc gaagtgcgcg
     1021 tgttccagtt ctggttctgg ggcacgtacc gccgcggccg gatctcgctg accgctgacg
     1081 cggccgagcg ctaccgcaac ggcggcctgt cggtcggctt ctggcactga ccgcgcgctg
     1141 gcgcacgaac taagcgcgca tcgcggcgtc gatcgcggcg tggaaccagc gcgccggcga
     1201 ttcgagcgaa ccgaatagca gctcgtcgac cgcgcccgca tcgatgctgc gctgcgcttg
     1261 ctcgcagacc cacaaatcct cccgctgaaa cacgccctct tcgatcagct gccagctctt
     1321 gttccagtgt tcggtctcgt gtgcgcgcgc tgccggcacg agcatcaggt gctcccacgc
     1381 ggtgacatcc ggcgcgagcg gatggaccgt catgatcgac aggaagtcgg gatgctcgac
     1441 gaacacggtc gacggaaaca gcagcaggct cggcgtcgcg agctgacgca gatcgcccgc
     1501 gctcgcgaga tcactcggcg cttcgagtaa cgcgcgacgg cccgtgaccg cgcggatgtg
     1561 cgggccgact ggctcggcga gcgacgcggc atcgaggaag aagcgataga ccgagtcgcg
     1621 gtgcaagacg cgaatgtgat agccgtcgag gaacgcttcg accacgagct tccagttgca
     1681 gcggcgcgtc gtcgtcgagc gttcccatag gacgtgctgg ttgagtccaa gcgctgcgac
     1741 gtcagcaccg agcggcccga gatagcaatc gaggtcgtcg gtgtccgaag cgccgagcca
     1801 cacgaggcca tcgcgctccg tcacgggcaa cggcgcgagc gcgcgcgtgt cgaggtcgat
     1861 gccgggaaac gaatcggcgt gcggcacgtg caccagcttg ccggtaaggt cgtacgtcca
     1921 cgcgtgatac ggacacgtca gcgccttcgc cgcgcatggc ttgtcgacga gccgcgtgcc
     1981 gcgatgccgg catgcattcg cgaacgcgcg cacgactccg tcagtgccgc gaacgaccag
     2041 cgcagctcgg cctggcagat caactggcaa gcacgcgccg gacacaagcg atgctgacac
     2101 cgcaacgatc cgcggcccac cgaagagcgc ctgctctcgc tcgcagcgcg cggcatcgcg
     2161 atagcgcaac gcggacactg tgaacggcgc agcatcctca gcggtcgcgc agttaccggc
     2221 ccgagcctga gcacgcagct tatctgccaa gcggagggtc gtcgtcggtt ccattgcctt
     2281 tattttgtat acgagataat tatctcgtat gcaagacgat cgtctacgca gctcgcttgc
     2341 caacgcggtc gtacggttgt tccggcaggt caatcgcgta cacaaccggc tgttcgcgga
     2401 ccatgacgtc tccgcggagc aggcgcacat cctgctcatc ctcggcgtct atggcccgat
     2461 gacgatcggt cggctgcaga agcagctctc gctgtcgagc gcgacgctca cgggcgcgat
     2521 cgatcggctc gaagcgcagg agcttgtccg ccgcgtgccg tcgccgcgcg atggccgcgc
     2581 gttcgtgatc gaatcgaggc tgcccgaccg caagcgcgca cagctcgagg cgatcgtcga
     2641 gaaaggcgat cagcgctgct tctcggcgct gaacgcgggc gagcgcaagg agctgctcag
     2701 gctgctcgag aagtgcgccg cgcaactcga ggagaccgcc gccgccaagt gatcgcgacg
     2761 agcgcgatca gcacgagcgc gccacgctga tcgccggacg cctcgcagca accgccacca
     2821 ccgccgcctg gcggcttcgt gccgccgtca tcaccgacgc ccgcatcggc atcgccggtc
     2881 ggtgcgttcg tcgcgcaacc tttgttgcct tgacgatcgg tcgcacagac ttgatacgtc
     2941 gcgctcatcg cctcggttgg aattgccgcg cgccacagca tctcgccgta ccactgcatc
     3001 gggatttccg tcgagccgac gacgaccacg accttctgga agtcatgcac gtgcgacggt
     3061 ccgacaagat cgttgacgcg tgcggtgagg atgttgccgc tcagcgacgt cgcgacgcgg
     3121 acgatcggcg gcgtcgtatc gaccttcacc gccatcgatg cgagctgcac cttgtcgggg
     3181 aacgcgacct cgccctgccc ttgcaggatg tcgatgcgac catcgccgtc gaagtcggcg
     3241 acggcgagac cgagtgtcgc gttcgtgtcg ttcggcgtcg cgttcgcgac gagcgtgaac
     3301 ttgccgctgc cgtcgttcga tagcaggcgg tccgcgccga gcgtgccgat caggagatcg
     3361 ggatcgccgt cgtcgtcgta gtcgagccac accgcgacgt tgtcgtccgc gttcgcggga
     3421 ttcgcggtgc cggtgaggcg catcgaggtt tcgtcggcaa acgtgccatc gccgcgattg
     3481 acgaagatgt gctcggtgac ctgcggaccg tcgttgatcg tgatgagatc gaggtcaccg
     3541 tctttgtcga tgtccatcgg ctcgaattcg tagttgttgg tgaagtgcgg cagcgcgttt
     3601 ggatcgtcgg tgaagtgacc ggtgccgtcg ttcttgaaca cgtagctcgt cgtgcagcgc
     3661 ttgcacgaaa cgagcacgtc gagatcccag tcgttatcga cgtcggcgag ctcgatgtcc
     3721 cacgaccact tcacgagtag atcgggcatc tgagtcgccg tcgcttcggt gaacgtgcct
     3781 gcgttgttga gatacaggcg cgtgcgaccg ccggtgttcg tgcccggatt cgtgccgccc
     3841 cagtcggcga gcacgatgtc gaggtcacca tcgttatcga catcaccaaa ttcggcgtcg
     3901 ccgatcgacg tcagctgctg cggcagctgt gctgtcgcat ccgtccagct ggcagcgtcg
     3961 cgcttgtaga gtttgagttg cgtctggtac gcgccgccgg tcagcagatc gagatcgccg
     4021 tcaccgtcga tgtcggccgc cttgatcatg cgtgacatcc cggtaaaccc gccgagcgcg
     4081 gttgcggaaa tctcggtgca gttcgtcgtg ccgccccagg tgttcttgaa gatgcgcgtc
     4141 ggctcgttgt tcggcgggtt gcctggactc gagtaatcac cgccgtttgc gagcaggaga
     4201 tcgaccttgc cgtcaccgtc gacatcggcg acctcgacct tgttcgtcca ctcggcagtc
     4261 gtgcctatgc agtttgcggt cgcgtcttgc caggtctgag ccatcgccac gccgggggca
     4321 accgtcacca tcaccacgat caggagtctg cgcatcgccg ggacaacatc acggctttgc
     4381 gctcgctgcc agtaacaagt ggctgcgcca gttgcgcatc tgggtatgcg cccccgacgc
     4441 ctcgatatgc gagcaggctt gagcttttgc acgtgacctc agggcatggt cggtgcaacg
     4501 gtacagccca tgcgacgccg aacgtttctt ggcctcgtgg ggggagcgag tgcggcagcg
     4561 attgccggtc cgtacttcat gcgccgagcg cacgcacaat ttggtgcgtt ccccggaggt
     4621 gcggaagcgg ccacgctgcc cgagggcctg cgcgcaaagc gcgtgctcga agtcttcctc
     4681 tacggcggtc tctcgcaatg ggagacgctc tacttcgttc gcaactacgg cacgccgact
     4741 gacgcgacgt atccgaacca gcagtactac acgtacgcgt ccagcaacgc gacgatgctt
     4801 cctcgttgcg gcacggccag cttcgacacc gtgcgcgact tcggtcagga cgcactcggc
     4861 gcgaacgtcg aaatcggtcc gttcgccgat cgcctctggg ggcgccagga cgtcgtcgat
     4921 cgcatgcgca tcgtcgtgca gaagcacacg ctcgagccgc acgaggccgc ggtgccgcaa
     4981 gcactcaccg gtcgtcccgt tggccagccg accgcagcgg gtctcggtgc gcacgtgcaa
     5041 cgcgcacgac tcgacggcgg cgtcacgccg gatcgcgcat cgccctactc ctacgtgttc
     5101 gcgaccggcg gcatctcgag cgataacgtc gcggcagcgg cggcgcctgg cgtgcatccc
     5161 ggcgctgcgc gaccactgct gatcaaaacc gacaacgcga ccacgttcac gagcctgctc
     5221 ggtcgctcga ccgtcggcgg cgcgcgccaa aaccacgacg cgctgatctc cgcgtacacg
     5281 aaccagtaca acgctcgcct cacgtggccg aacggcacgc gcgtacgcag cgcgcgcacc
     5341 gacgacttcg aggtcgcgtt caacaacgcg aagcgtgtcg acgcgatcag cgcagtgatc
     5401 gcgcaggatc tgttcaacac gcagggtggc acgtcgtgcc agacgacgaa gtcgcgcgac
     5461 atcccgctca tcggcttgtc ggcggccgcg cgcctgctca cgcatcccac cgagccggcg
     5521 tcgtatgtgt gcgtcagcga cactggcctc tacgaagcgt cgggtggcgg cggttacgac
     5581 acgcacaccg acaacgcgcg tgacaccgcg acgaacttcg acaacatgct caagtcgttg
     5641 ctcggcatca tcaacgcgcc aggcgagacc gacccgaaga agatctcgct cgacgacacg
     5701 ctgatcatcc tcaacaccga gttcggccgc acgccccgtg cgcaaggcac gaccggccgc
     5761 aaccatcatc cctacggcta cgtcaccgcg ttcatcggtg gcccgacgac gaagggcgtc
     5821 tccggcgcga tcggaccgaa cggcatggcg gacatgtacg cgacgccggc gcagaaccgg
     5881 atcgcggcgc tcctcgcact cggcatctgg ccgttctcgc aagaagcgtt cgccgtctcc
     5941 gacgtaccgg gcgcgacagg tgaagtcgat gctgcgctca aggccatgca gatgtgcctg
     6001 gggaggacgg catgaagcgc ctcgctgtca tcgcgatcgt cctcgtcgcg tgcggcaccg
     6061 atcaccgcaa gcccgacgac ggcgacgaca cccaggacgt gccgtacacc gagtgcggcg
     6121 gcgatccggc gtcgttcgtg cgccaggcgc tcgtgtcgct cgacggtcac cggccgcgca
     6181 gccaagccga ggtcgacgtc tatgtcgatc tgtacaacgc ggtgaagacc gcaggcggcg
     6241 atccgaagga gacggtctcg cgcgcgatca tggagcgccc cgagttcgcc gatcgctgga
     6301 tcgacacgtt catggacgcg atgcacgtcc agcgcatcga cgttcagaac gaagcaagct
     6361 gctgggacaa ggccgtgcgc accaccgtcg atgcgggcct cgcaaccgcg atccgcatgc
     6421 aggccgcgac gacgagcggc gatggcaagg gcgcgtggac gatggccgac ctcgcgcgct
     6481 cggcgatggt gctcgacgat ccgacaccgc tgtatcgcgc gcagatcttc tcgatggtcg
     6541 cgcatccgat tcccgccgcg aacgtgccgg cgaaggaagc cgagctcgcc cgccgcgctg
     6601 atttcggcga gacgttcgac tcgggcttct tgcatcgcga cgtcgtctgc ctcgcctgtc
     6661 acaacagcga gcgctcggtc accgacagcg acgatccagt gacggatcgc cactggccgg
     6721 tgcccggtat gccggagaag gccgtatacg gcacctcggc gggcatcgag atggaccgcg
     6781 cgcacgctgc gttccgcgtc gatggcttcg tcggcggtaa cagtcatccg tggggttggg
     6841 acgccgattg cggcgggttc tcgacgacgg ttggcgacga catcgccggc gtcgacggca
     6901 agctcgcgag cctcaccggc aagcgcatca ccgtgtacgg cctcgagcag gcactcgcgc
     6961 gcggcttcga caagctgcgc ggtcagctgc cgccgatcgg catggacggc gcgatcgcgg
     7021 atccggacgc cgcgctcgcg tggctcgtca cgctgaagat caccgaggac gtctggaagc
     7081 gcgcgaccgg cacctcgctg acgatcgcga actacttccc gcgcaaccag gcctcgagcg
     7141 acctcctcta cagcctcgcc acgaagttca cgcagagtgg ttactcggtg aaggcgttgc
     7201 tcgtcgcgat cgtgcagagc gactactaca atcgccaacc gccggagctc ggctgcggca
     7261 aggaaccgta cacgtatccc gctgtgttcg atccgtgggt cacgtccgac gccgaccccg
     7321 agcgccgctt caacggcccc ggtgacgcgg tcactgcgct cgacgcacgc acgctcgtca
     7381 ccgcgacgaa cgctgcgctc gagtggccgg caccgcccgc cgcgtcgcgg ttccccgatt
     7441 acggcgacgg ctgcgagaac gacacgtgct cgcagatgca gagctcttgc aaccagttcg
     7501 gcgggtgctg caactcgtac aaggccgcct gcatgatggg cggcgtgatg ccgtcgatcg
     7561 aagtgccgtt cgagcgcggc gtcggcatgt tcctgcgcaa tagcgagcgc ggcttccgcg
     7621 gcctcgattt ccaggctcgc ctggtctggg aagaccgcac cggtgcgtgc gcgcgcccga
     7681 cgtgggcgag ctcggacttc atcgacaaga tcgtcgccgc cggcgcagcc gacacgacag
     7741 ccaccgtcgg tgacgtcgcg gcagcgatca aggatcgcct cattggcgag tccgcgatcg
     7801 tcgacgctca ggagatcgcc gcgatcaccg gcctgctcgg cgccgcgcca gagatgccgg
     7861 caagcaatgt caccgtggcg gccgcccgcc agttctgcgg cgcgctgctc ggttcgccgc
     7921 agttcctact gcaaggcatc gcgggccgcg gcggcgaccg cccgaagctc acaccgacgg
     7981 acgtcggcta cgacgcgatt tgcgccgacg tcgcgaccca tgtcgccggc aagaccgtgt
     8041 cgtgcacggg cggcaagctc acggtccagt agtagaaatc accagcggtt gcaggcgcgt
     8101 ggcgtttgcg ccatgttcgc ggcagctatg catctccatc gtttcggtgc tgcctgtttc
     8161 gctctcctga tcacctccac catggcttgc gagaacaaga gcaagcccaa agacaccggc
     8221 gaagccgcgc aaaagccggc cggtaacgag acgccgacgc cggtcaacaa ggagcccgag
     8281 ctgccgaagc agccgagccc cgatccaacg ccggccgccg acgtgcgccc gccggttgcc
     8341 gcggatctcg cggagtacac gaaagacatc ccgggcaccg gcacgaagct gatggccaag
     8401 atcgagacgt cgatgggcac gttcaactgc gagctctacg gcgacaaggc gccgatgacc
     8461 gtcgcgaact tcgtcggtct cgcgaccggc aagaagccgt ggctgagccc gagcgggatg
     8521 cagaaggaca agccgttctt cgacggcctc acgtttcacc gcgtgatccc gggcttcatg
     8581 atccaaggcg gcgatccgcg gggcgatggt accggcgggc cgggctacaa gttcaacgac
     8641 gagatctggc aaggccagca catcgagccc ggcgtgctcg cgatggcgaa cgccggcgtg
     8701 cgcggcggcg aaggcacgaa cggtagccag ttcttcatca tggaaggcgc gcgtccagat
     8761 ctcgacacgc accacaccgt gttcggcaag tgcaaggagc tcgacctggt caagaagatc
     8821 gagaacgtgc ccagcaccga cgacaagccg aacacgccgg tcacgatcaa caagatcacg
     8881 ttctcgaagg cgtaatcggc gacgtaaccg ctgcgcgtcc ggcacgcact gttccggcat
     8941 ggtcgccagc gcccggtttc tcgcctcgtt cctcgcggcg tcgctcatca ccggctgtgg
     9001 cgcggctcgc cccgcccggc cgatgcagct gcagcgagtg attctgtacc agaacggcat
     9061 cggctacttc gagcgcgccg gtcacgtgca gggcgagacg ctgcggctgt cgttctcgcg
     9121 cggcgagctc gacgacgtgc tgaagacgct gacggtgatc gaccggctcg gtgccggcgt
     9181 cgcgaccgtc gacgtaccga cgctcgacga caaagcgacg tcgatcggcc tcggcgtccg
     9241 catgagcgcg ggccgcgtgc atgacgtgaa ggtcagctat gcggtgccga caccgacgtg
     9301 gaaggccgcg tatcgcgtcg tgctcgacga caagacgaag gcgcaaagcc tgatccaggg
     9361 ctgggcgatg gtgaacaacg cgtcgcagga agactggcgc gacgtccagc tcacactcgc
     9421 aaccggcgcg ccgatgtcgt tcgtgcatga cctgcacaca cccgagtacg tgaagcggcc
     9481 cgacatccac ggcaacctcg tcgcgccgat cgtgaccgga ccgatcgaca gcgagaaggt
     9541 cggcgccggt gatagcgact ccgacggcat tcctgacgtc gacgacgtgt gccccgacag
     9601 ccccgaggat cgcgactcgt tcgaggacac cgatggctgc cccgacgccg acaacgatcg
     9661 cgatcgcgtg aacgacgcct acgacaagtg cccgaacgag ccggagacgt ggaacggctt
     9721 cgaggatgac gacggctgtc cggatcgcgg ccgcgtcgtg gtgacgagct cggcgatcga
     9781 agtgctcgag cagatctact tcgcgaagac gagcgatggc atcaagaagg agtcgggtga
     9841 gattctcgac gcgatcgcgg cgacgctgcg cggcaatccc gacatcgtca aggtcgaggt
     9901 cggcggccac gcgagcgacg acgaaggcga agcgtgggcg ctgtcgtcgc ggcgcgcgtc
     9961 ggttgtgcgg caggcgctga tccagcgcgg cgtcgagggc aagcggctcg tggtcgtgcc
    10021 gtacggttcg acacggccgc tcgatcccaa ggcggcggac aagaaccgcc gggtcgactt
    10081 cctgatcgca cagcgccagg aggaaatgca cgcgatgccg acgcggcgcg cgccgacgcg
    10141 catcgatacc cagacagcgg cggcgagcgt ccacacggcg accaagccgg ccgaggtcgc
    10201 gggcaccgtg cgctacgagc tgaccgagcc ggtgtcgatc cgcaagggct cgtcgtcgat
    10261 ggtgtcgatc ctgaacaagt cggtcagcgg cgaggacgtg tacttgttcc ggcccgatcc
    10321 gaatgcaccc ggcagcgcgc ggcatccgtt ccgcgcggtg cgcctcgtca acgactcggg
    10381 ctacatgctc gaacccggtc cgatcgcgat cttcgcgcgc ggcacgttcg tcggcgacag
    10441 catgatcggg cggctcaacg tcggcgagac ggcgtggatt ccgtacgcgc tcgacggcgc
    10501 gacgcaggtg accgccgcga ccgacgaggc cgagcggccg atcaagatcg tgtcgatcca
    10561 gcgtggcgtg ctcaccgtcg agaacgcggg cgtgcgcgtc accaagtatt cgatcgcggc
    10621 gggtgccgat ccggcgaagc agatctatct gcgccacgac aaggcatacg ggttcgcggc
    10681 gaaggacctg ccgccgggca cgcaggatcg cggcgactcg tatctcattc cgctaccgct
    10741 gcaggcgaag aaaacgagcg tgctgtcgat cgaagagcgc cagccgcgcc gccacacgat
    10801 gcacatcctc gatgcaggcg cgaccgagat cgggctctac gtcgagggct cgcacctggc
    10861 accggcgatc gaagcgaagc tcgaggaagc gatcgagctg cgcaaggaga tgggcaagat
    10921 cgaagaaggg ctcgacggtc tgcgcgagcg catcgtcgac ctctcggagc gcgccgacga
    10981 gatccgcgag aacatccgcg cgctcgacaa ggtgcgcggt gccgatccgc tgcgcaagaa
    11041 gctcgtcgcg agcctgaccg cggtgacgac cgaagcagat gcgctcgcgc gcaagctcgg
    11101 cggtgagagc gaagcgctcg cgacggcgcg caacaagctg caggacgcgc tgcgcgagat
    11161 caacctcgac gagaagacct aaccgaagat cttcatcgag tagtggtcgg cgcccgagtc
    11221 cttgcccgag ttcgcgttcg tgacttccgt gaacggcgcg tagcagtgga acgtgagcac
    11281 ctgaccttcg ccggtcagca tgaagatcat cggccgatcc cagttgaacg agtcggggtc
    11341 gtagacatcg aggaaccgga agctgagcgc gtgcgactcg cccttgccga tgctcatcgc
    11401 cttcgtgacg ctcgtcttgt tcgtgatgac cgagagctcc atctggtcct tctcgatcca
    11461 gctgtacttc gacgcgcagt tgacctgcgc ctcgaagctc cagttgagct tcttgatcgc
    11521 gctcatcgca aagccgtacg acgccgggtc cttcaccttc gagatcttca gcgactgcag
    11581 cagcatgtgt cgcggcgccg cgagcgcctg cgcttcgtcc gcgaacgacc accactcctt
    11641 ctcgttgacc ggcaacttgc cgtcggtgac cgacgcgtgt tgatcgggcg acgccgtgtg
    11701 cgtgacctcg gcgccctccc ctttgtgttc cttgtcgagc ttcatgctcc acgggatcga
    11761 ccactgctcg gagacgagcg gcgcggtttc gccaccgcgc tgcacggccg ccgtggtcac
    11821 gaacttttcg tggccctgga tctcgtcgag cacggcgtcg ccgaccttga ggttcagcgt
    11881 ctgcgacggc ttgtccttga cgagcaggac gttgtccttg aactgatcgg tcggctcgag
    11941 cgtgacgctg tcggtgtacc agggaccgcc tttgcccttg ctgccgcccg tcttcgagtt
    12001 gtcgcgatcg acgtacggcg tcgctgggcc acccggctta tcgttcgggt tcgtcgactg
    12061 gtcgtgcatc tggccggtct tcatcgtctg cgtgccggcg accgagccgt cggcacggcg
    12121 atagaccgcc ttccgctccg attcgagaac gttctggatg ccgcctgctt cgatgt
//
LOCUS       DASUFX010000681         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_803772,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000681 DASUFX010000000
VERSION     DASUFX010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803772"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFV99_27485"
     CDS             <1..499
                     /locus_tag="VFV99_27485"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5063161.1"
                     /translation="LPYFFATPVLAVDTQRKWLYVAYARGSRDAAWDLVIAASKDSGA
                     TWKRSTLAGDGCALHMVPTLALDAATGTLHIAYYDSEGAPGRFAHATCGPGVTKCKLL
                     GAISSVTAPVLSLARSTTKSVGDYESLLVDNKRKILHAVWAQSVDEAGKPVTRIFHAA
                     AKLKK"
     gene            524..2356
                     /locus_tag="VFV99_27490"
     CDS             524..2356
                     /locus_tag="VFV99_27490"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX5063162.1"
                     /translation="MSSGMDSGHGTDPHDSLPGVIEKPAGATEFSIRAVIAGVVVAAI
                     MGASYPYIVLKLGFGPNVSVVAAFFGYLFLGILFRNFTRWENNIVQTAGTSASQTAFM
                     CVILAAFDLMVQGHPGTFTFRPAWYESFLWLMFASILGILMAVPLRKHYVVDEKLTYA
                     DGVAAAETIIVLDARGKDAKRAAIALFAATVASALVWLLTTKYGARLFHVAPTAEREG
                     AIVDTVFATMGGLTAATAGAGFSVSLLSIGSGMIVGNRVNISMAIGATIAWIIGPEFL
                     VPDVIAHPVRRDLLLWMMWPGTGMLIAAGLTALALKWRTLKRTFSTLSGDAIKGGDVP
                     LKLIGIGVGLASIGLVVVQYVFFDLPIWLTIIAILLSAPLMLVGLRVLGETNWGPISQ
                     LTNMMQAIFAALVPKNLIANLGASGTTGTIAVQSEAIMQDYKAGHIIGSTPRFLTYSQ
                     LIAAPIGALAVALVYPLLRDTYGIGAMAVPGQTPLSSPISVRIVGFADVLSGGVDALP
                     DYALKFMVIGAVLGIVITLGETYIKGGYKWLPSATGLGIGMMVPGTVVFTMVLGGIVM
                     SIWNRTNKASADKLAMPLASGLIAGEAVMAIVIPVLIALHAISP"
BASE COUNT          386 a          804 c          775 g          434 t
ORIGIN      
        1 gttgccgtac ttcttcgcga caccagtcct cgccgtcgac acccagcgca agtggctcta
       61 tgtcgcgtac gcgcgaggca gccgtgacgc ggcgtgggac ctcgtgatcg cggcgtcgaa
      121 ggatagcggc gcgacctgga agcgctccac actcgccggc gacggctgcg cgctgcacat
      181 ggtgccaacg ctcgcgctcg acgccgcgac cggcacgtta cacatcgcgt actacgatag
      241 cgagggtgcg cccggccgct ttgcgcacgc gacctgcggc cccggcgtca cgaagtgcaa
      301 gctgctcggg gcgatcagca gcgtgacggc gccggtgctg tcgctggcac gcagtacgac
      361 aaaatcggtc ggcgactacg aaagtctcct cgtcgataac aagcgcaaaa tcctgcacgc
      421 cgtctgggcg cagagcgtcg acgaggccgg caagcccgtg acgcgcatct ttcatgctgc
      481 ggcgaagctg aagaagtagc cgctcgctgg taggttccga ccgatgagct ccggcatgga
      541 cagcggccac ggcaccgacc cgcacgacag tctccccggc gtgatcgaaa agccggcagg
      601 tgccaccgag ttctcgatcc gcgcggtcat cgctggtgtc gtcgtcgccg cgatcatggg
      661 tgcgtcgtac ccgtacatcg tgttgaagct cggctttggg cccaacgtgt cggtcgtcgc
      721 cgcgttcttc ggctacctgt tcctcggcat cctgttccgc aacttcacgc gctgggagaa
      781 caacatcgtc cagaccgcgg gcacgtcggc atcgcagacc gcattcatgt gcgtgatcct
      841 cgcggcgttc gatctgatgg tgcaggggca ccccggaacc ttcacgtttc ggcccgcctg
      901 gtacgagtcg ttcctctggc tgatgttcgc cagcatcctc ggcatcttga tggcggtgcc
      961 gctgcgcaag cactacgtcg tcgacgagaa gctgacgtac gccgacggtg tcgctgcggc
     1021 cgagacgatc atcgtgctcg acgcgcgcgg caaggacgcg aagcgtgcag ccatcgcact
     1081 gttcgcggcg acggtcgctt cggcgctcgt gtggctgctc accacgaagt acggcgcgcg
     1141 cctgttccac gttgcgccga ctgcggagcg cgagggcgcg atcgtcgaca ccgtgtttgc
     1201 gacgatgggc ggcctcaccg cagcgaccgc cggcgccggc ttctcggtca gcttgctcag
     1261 catcggttcg ggcatgatcg tcggcaaccg ggtcaacatc agcatggcga tcggcgcgac
     1321 catcgcgtgg atcatcggcc cggagttcct cgtccccgac gtcatcgcgc acccggtgcg
     1381 gcgcgacctg ctgctgtgga tgatgtggcc cggcaccggc atgctcatcg ccgccggcct
     1441 caccgcgctc gcgctcaagt ggcgcacgct caaacgcacg ttcagtacgc tgtccggcga
     1501 cgcgatcaag ggcggcgacg taccgctcaa gctgatcggc atcggcgtcg gcctcgcgtc
     1561 gattggcctc gtggtcgtgc agtacgtgtt cttcgatctg ccgatctggc tcacgatcat
     1621 cgcgatcctg ttgtcggcgc cgctgatgct cgtcggcctc cgcgtgctcg gcgagacgaa
     1681 ctggggaccg atcagccagc tcacgaacat gatgcaggcg atcttcgctg cactcgtgcc
     1741 gaagaacctg atcgcgaacc tcggcgcatc cggcacgacc ggcacgatcg cggtgcagtc
     1801 cgaagcgatc atgcaggact acaaagccgg tcacatcatc ggctcgacgc cgcgcttcct
     1861 cacgtactcg cagctgatcg ccgcaccgat cggcgcgctc gcggtcgcgc tcgtctatcc
     1921 actgctgcgc gatacctacg gcatcggtgc aatggccgtc cccggccaga cgccgctgtc
     1981 gtcgccgatc tcggtgcgca tcgtcggctt cgcggatgtg ctgtcgggtg gcgtcgacgc
     2041 gctgcccgac tacgcgctca agttcatggt catcggcgcc gtgctcggca tcgtgatcac
     2101 gctcggcgag acgtacatca agggcggcta caagtggctg ccgtcggcga ccggtctcgg
     2161 catcggcatg atggtgcccg gcacggtggt gttcacgatg gtgctcggcg gcatcgtgat
     2221 gagcatctgg aacagaacga acaaagcaag cgcggacaag ctcgcgatgc cgctcgcatc
     2281 cggactgatc gcaggtgaag cggtgatggc gatcgtgatc cccgtgctga tcgcgctcca
     2341 cgcaatctca ccgtgatacg ctggtctcaa tgaccgttca tctgcagctc ggtggggga
//
LOCUS       DASUFX010000682         8524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1081859, whole genome shotgun sequence.
ACCESSION   DASUFX010000682 DASUFX010000000
VERSION     DASUFX010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081859"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFV99_27495"
     CDS             <1..622
                     /locus_tag="VFV99_27495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063163.1"
                     /translation="ESNRSVFLSAITDEQLDGRARVQAITELVAADDKLEKDARAAIV
                     RATRSADCRVAAGAAQFLVRNGDKKFAPAKPHTGKPDAMMRALCVLASFEAGQRADEP
                     SFLLGYVPKKGLELVTTTYDEYNDVDTDGDGDPRTEKTAVQVPRNEVTLPETEDMIRA
                     FAHCKGTTCRSADREFRFVFKGADLLLTRLEVAERPPCNTPAITNP"
     gene            669..965
                     /locus_tag="VFV99_27500"
     CDS             669..965
                     /locus_tag="VFV99_27500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063164.1"
                     /translation="MLRWLGIILAIALVSTSADAQVFKPKSKKAAAAKKESSAAPEKK
                     SAKKQPRTASTKKRVSKKKAAASDRASSDEETDEPESKGAGKDYVKIWDDDDIE"
     gene            1188..1973
                     /locus_tag="VFV99_27505"
     CDS             1188..1973
                     /locus_tag="VFV99_27505"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX5063165.1"
                     /translation="MIADACGRALRRTLPALIALFILAAIGGTAWAGYRFVTTSSRFA
                     ITTIEVNGVEQLTADQIRALSAIRIGDNVFATNLDAVTHEVRENPWIASAEAHRVLPH
                     TIVIDVREHVAAAVVQLGGLYLVDADGHPFKRAELGSEDGAGLPIITGLDRETYLANP
                     AGTAAQIRSAMAALVKWRGVEDRPSVGEVHLDPHGALTLVTYEHAIAIQLGDMDAALD
                     QRMQTFDATWAELTSAERARTRAIHLDARADQVTVAFAPTDHT"
     gene            1980..3209
                     /gene="ftsA"
                     /locus_tag="VFV99_27510"
     CDS             1980..3209
                     /gene="ftsA"
                     /locus_tag="VFV99_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5063166.1"
                     /translation="MAKPKNSEIIVGLDIGTTKIACIAGEVTEDGVDIIGIGTAPSKG
                     LRRGYVVNIDATVSSIQQAVDEAENMAGCEISTVYAAISGAHVRGLNSHGIVAVKDKE
                     VRDADIARVIDAAKAVAIPMDREVLHVLPQQYVVDDQDGIRDPLGMAGVRLEAKVHIV
                     TTAVASAQNVIKCANRCNLQVADIVLESLASAQAVLEDDEKELGVALIDIGGGTCDLM
                     VYCDGAIVHTSVLPLGGGHITNDIATVLRTPLDSAEKIKRKYGCAWHGAVEDGETMEV
                     PSVGGRGPRVLPRIKLVEVIEPRVEEIFEHVKKELMRSGYFDSLAAGVVLTGGATAMD
                     GVCEIAEQVLMLPTRRGTPTKIGGLVDVVRSPSYSTGVGLVMFGADQGRTVQLPSRSE
                     DRPGLFKRAWGRLAEMF"
     gene            4010..5428
                     /gene="ftsZ"
                     /locus_tag="VFV99_27515"
     CDS             4010..5428
                     /gene="ftsZ"
                     /locus_tag="VFV99_27515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5063167.1"
                     /translation="MALIEFDDVSHAKILVIGVGGGGGNAVNTMISGNLDGVEFVVAN
                     TDMQALEANMAPHKIQLGNALTKGLGAGANPEIGRRSAEESMQNIADLISGADMVFVT
                     AGMGGGTGTGAAPVIAQIARDCGALTVGVVTKPFGFEGKKRSRQAVEGIERLNAAVDT
                     LIVIPNNRLLALVGQNTSMVEAFRKADSVLLNAVQGISDLMTVPGLINVDFADVRTIM
                     AGMGRALMGTGIGSGKKRATEAAETAISSPLLEDVSIEGATGILINITGGPDLTLHEV
                     NEASSLIQQAAHEDANIIFGSVIDPNLSDEVRITVIATGFDRGAKTEVIIEPSRSAPN
                     TVARKPSQTMLPYDVSSQITKEYPPVPVAPRMPAKVQAQIIDEPDVQIEWATPDLNEM
                     SEIERALSDYSEPVPEGEPSQRMATGSGPATPSDGNPLVHPDRKRAQSRPSMKAVLAG
                     EIDTESELDVPTFIRRHNTSHG"
     gene            5503..6081
                     /locus_tag="VFV99_27520"
     CDS             5503..6081
                     /locus_tag="VFV99_27520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063168.1"
                     /translation="MLTESAARWMLVLHTIVGVAAVGAATHLVLWLRKYVRGQSGRRR
                     AVLRFAWLVLALQLSAFIAGNVMYPTYKVEVRAAYLENAPAITASEQAKQRELGKLAA
                     REHAEASEPAATVEIVKRAAQAARWFDVKEHWIALGLFVSAALILVLSLWDPKGEGRV
                     IAPIVMSLAVIVAGTVWFGAIVGVLTASWRAV"
     gene            6104..6436
                     /locus_tag="VFV99_27525"
     CDS             6104..6436
                     /locus_tag="VFV99_27525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063169.1"
                     /translation="MRILLALTVFAFACGGTKPKTESSMVNEGSDQAPTCCCKTIPQV
                     GEKEIVPNYNMQGRMECSAANGECVDDVQCNKTGEGGTEVPASTGTGGPGEPPPPPVL
                     EPSTSSTP"
     gene            complement(6701..7432)
                     /locus_tag="VFV99_27530"
     CDS             complement(6701..7432)
                     /locus_tag="VFV99_27530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063170.1"
                     /translation="MRNFRRFRIARWLLLCLTFTGGGVAWADEATHKADFYIEDPYAP
                     HHTTGSEARIGSAVGFLYHEPVDVTAVGGNAAIGYRFGRFTIEAEYTYLAFQVKGPDD
                     TPLGAGQRLSALGRLDVIRLGPRIVGQNSLLSIYVEGGAGTAWNHWYKPAYDEASRLV
                     PDDTRRPEGLVGFGIALDHRLQEPIGFPHRVGWFLGWRVAMSPHQQMVGSICRGESCK
                     PVEMMDEDRMIDRSMLFQSSLAFTF"
     gene            complement(7464..8282)
                     /locus_tag="VFV99_27535"
     CDS             complement(7464..8282)
                     /locus_tag="VFV99_27535"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5063171.1"
                     /translation="MTISGTTRIAAVLGWPVDHTRSPTMLNAAFSADQIDAVMVPIGV
                     PPDGLATVVAALRAMKALGASVTIPHKLAVAQLCDELSPEAKAIGAVNCLQFGDQLVG
                     HNTDCDGFLDAVIAAGCELRGKRVILLGAGGAARAVAYGVRSARAIEVVARKPAEVSW
                     AQAWPWTEENLRDCFARADLVVDCTSAGMDPEADAAFTDALPLDALPPGAWVSTLVYH
                     RRTNLLERASSLGHSTLDGRAMLVHQGARAFAIWTGTPAPVAVMARALDASLAR"
     gene            complement(8279..>8524)
                     /locus_tag="VFV99_27540"
     CDS             complement(8279..>8524)
                     /locus_tag="VFV99_27540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063172.1"
                     /translation="FLVLLPYTDLKGSANLGRRVIAAVAEGDPVVAGGRSFPPRVVGA
                     VAGGAPGQALSFAKLMKDATRALDQARRDGAELAVQP"
BASE COUNT         1578 a         2821 c         2797 g         1328 t
ORIGIN      
        1 cgagagcaac cgctccgtgt tcttgtccgc gatcaccgac gagcaactcg acggccgcgc
       61 gcgcgtgcaa gcgatcaccg agctcgtcgc tgccgatgac aagctcgaga aagatgcgcg
      121 cgcggcgatc gtgcgtgcga cgcgctctgc cgattgtcgg gtcgctgcgg gtgcggcgca
      181 gtttctcgtg cgcaacggcg acaagaagtt cgcgcccgcg aaaccgcata cgggcaaacc
      241 cgacgcgatg atgcgcgcgc tgtgcgtgct cgcgagcttc gaagctggtc aacgcgcgga
      301 cgagccgtcg ttcttgctcg gctacgtgcc gaagaaaggc ctcgagctcg tgacgacgac
      361 gtacgacgag tacaacgacg tcgatacgga tggcgatggt gacccgcgca ccgagaagac
      421 cgcggtgcag gtaccgcgca acgaagtcac gcttcccgaa accgaggaca tgatccgcgc
      481 gttcgcccac tgcaagggca ccacgtgccg ctccgcggac cgcgagtttc gctttgtctt
      541 caagggtgcc gacctgctgc tgacccggct cgaggtcgcc gaacgacctc cgtgcaatac
      601 gcctgcgatt acgaatcctt gaccgcggaa actagatctc ccccgtggct gtggcacgat
      661 aggcggcaat gctgcgctgg ctcgggatca tcctagcgat tgccctggtc agcacgtctg
      721 ccgacgcgca ggtcttcaag ccgaagtcga agaaggctgc ggcagcgaag aaagagtcgt
      781 cggccgcgcc cgaaaaaaag tcggcgaaga agcagccgcg caccgcgtcg accaagaagc
      841 gcgtgagcaa gaaaaaagca gctgcgagtg atcgcgcgag ctcggatgaa gagacggacg
      901 aaccggagtc aaaaggcgcc ggcaaggact acgtcaagat ctgggacgac gacgacatcg
      961 agtaagcgca tgtgcgcatg acgcaaagtg atccggagcc ttccaaaaaa ttgctccgcc
     1021 gctagccgca tgcgtatttg cgaagcatgg atcgatcccg acggtcgggt gcgaaggaga
     1081 cgcgcccgca gccgccagcc gcctcgcgtt tgacggtcaa agcccgtcca aaccggcgta
     1141 aggctgcgcc gcgcggatcg ctctggtcgc ggctgcccaa gccgatcatg atcgcggatg
     1201 cctgcggacg cgcgctgcgc cgcacgctgc ccgcgctgat cgctctgttc atcctcgccg
     1261 cgatcggcgg cacggcgtgg gccggctatc gcttcgtcac gacgtcgtcg cggttcgcga
     1321 tcacgacgat cgaggtgaac ggtgtggagc agctgaccgc ggatcagatc cgcgcgctgt
     1381 cggcgatccg catcggcgac aacgtgttcg cgacgaacct cgacgccgtc acgcacgagg
     1441 tgcgcgagaa cccgtggatc gcgagcgccg aggcccaccg cgtgctgccc cacacgatcg
     1501 tgatcgacgt tcgcgagcac gtcgcagccg cggtcgttca gctcggcggc ctgtacctcg
     1561 tcgacgctga cggccatccg ttcaagcgcg ccgagctcgg ttccgaggat ggcgccggtc
     1621 tgccgatcat cacgggtctc gatcgcgaga cctacctcgc gaatcccgcg ggcaccgccg
     1681 cgcagatccg cagcgcgatg gcagcgctcg tcaagtggcg tggcgtcgag gaccggccgt
     1741 cggtcggcga ggttcacctc gacccccacg gcgcgctcac gctcgtcacc tacgagcacg
     1801 cgatcgcgat ccagctcggc gacatggacg ccgcactcga ccaacgcatg cagaccttcg
     1861 acgcgacctg ggcggagctc acctccgccg agcgtgcgcg cacgcgcgcg attcacctcg
     1921 acgcgcgcgc tgaccaagtc accgtggcct tcgcgccaac cgaccacacg taagggacca
     1981 tggcaaagcc caagaacagc gagatcatcg tcggcctcga catcggcacc accaagatcg
     2041 cctgcatcgc aggtgaggtg accgaggacg gcgtcgacat catcggcatc ggcaccgctc
     2101 cgtcgaaggg attgcggcgc ggttacgtcg tcaacatcga cgcgacggtg tcctcgatcc
     2161 aacaagcggt cgatgaagcc gagaacatgg ctggctgcga gatctcgacg gtgtatgccg
     2221 cgatctccgg tgcgcatgtc cgcgggctca actcgcatgg catcgtcgcg gtcaaagaca
     2281 aagaagttcg cgacgcagac atcgcgcgcg tgatcgatgc ggcgaaggcc gtcgcgatcc
     2341 cgatggaccg cgaggtgctc cacgtgctgc cgcagcagta cgtcgtcgac gatcaggatg
     2401 gcatccgcga tccgctcggc atggcaggcg tgcggctcga agcgaaggtg cacatcgtga
     2461 cgacggcggt cgcgtcggca cagaacgtca tcaagtgcgc gaaccgctgt aacctgcagg
     2521 tcgccgacat cgtgctcgag tcgctcgcgt cggcgcaggc cgtgctcgag gacgacgaga
     2581 aagagctcgg cgtcgcgctg atcgacatcg gcggcggcac ctgcgacctc atggtctatt
     2641 gcgacggcgc gatcgtgcac acgtcggtgc tgccgctcgg cggcggtcac atcacgaacg
     2701 acatcgcgac ggtgctccgc acgccgctcg attcggccga gaagatcaag cgcaagtacg
     2761 gctgcgcatg gcacggcgcc gtcgaggacg gcgagacgat ggaagtgccg tccgtcggtg
     2821 gccggggccc acgcgtgctg ccgcgtatca agctcgtcga agtcatcgag ccgcgcgtcg
     2881 aagagatctt cgagcacgtc aagaaggagc tgatgcgtag cggctacttc gacagcctcg
     2941 ccgcaggcgt cgtgctcacc ggcggcgcga ccgcgatgga cggcgtgtgc gagatcgccg
     3001 agcaagtgct gatgttgccg acgcggcgcg gaaccccgac gaagatcggt ggccttgtcg
     3061 acgtcgtgcg ctcgccgtcg tactcgacgg gcgtcgggct cgtgatgttc ggcgccgacc
     3121 agggccgcac cgtgcagcta ccgtcgcgca gcgaagatcg ccccggcttg ttcaagcgcg
     3181 cgtggggccg tctcgccgag atgttctgag caacgagcgc tcgctgttta cagcaacgag
     3241 ggctcgctgt ttacagcaac gagggctcgc tgtttagagc gacgagcacc cgcgaggccc
     3301 ttggtcatcc ctgaagagga aaaaatcgcc gccaccggcg cgcctttgat gatgagggct
     3361 ggagcgcgcc ggcagcggca aagcgattcg tgcggtcagt cagccggctt gtagtcgtga
     3421 cgctgcgaca cgagttgctt tgagctcaaa gcatccgtgg ccgccgcgag ctcgatgccg
     3481 ccggacgccg tcacgctaac tagtctcgtc atggcggagg acctatcacg cgaaggtccg
     3541 atggttcaac cattttgtag tctatggata tcgttgcgaa atgtccgact gccggacacc
     3601 tctagaggcg cgaaatcaca gaccaagtga tcgcccctca gctcccggat ccggaccggg
     3661 gttagttaac tgctgcggcg ccgggccgga aaactaaccc cggtacgttg cagggtcgca
     3721 acgacctcgt cgggtcgtgc gctggccggc gctcggacac gctcggacac gctcggacac
     3781 gctcggacgc gtcggacgcg tcggacgcat cggacgtacg cagacgtgcg cagcgatcgc
     3841 gcagacctgc gcagcgatcg cgcagacgtg cgcagcgatc gtgcagcgat ccatgtgcga
     3901 tccacgctca ccaaaaatcc agcgcatctg cgcgcgaaga gcaaacaaaa agttgcccct
     3961 gatgatcgcg gcctttcata tgggcctacc aggggagcac aaaccaacca tggcactcat
     4021 cgaattcgac gacgtcagtc acgcaaagat cctagtcatc ggtgtcggcg gtggcggcgg
     4081 caacgcggtc aacaccatga tcagcggcaa cctcgacggc gtcgagttcg tcgtggccaa
     4141 cacggacatg caggctctcg aagccaacat ggctccgcac aagatccagc tcggcaacgc
     4201 gctgaccaag ggtcttggcg ccggcgcgaa cccggagatc gggcggcgct ccgctgaaga
     4261 gagcatgcag aacattgccg atctgatctc gggcgccgac atggtgttcg tgacggcagg
     4321 catgggcggc ggcacgggta ctggcgctgc gccggtcatc gcgcaaatcg cgcgtgactg
     4381 cggtgcactt accgtcggtg tcgtcacgaa gccgttcggc ttcgagggca agaagcgctc
     4441 gcggcaggcg gtcgaaggca tcgagcgcct caatgcggcc gtcgatacgc tgatcgtgat
     4501 cccgaacaac cggctgctcg cgctcgtcgg ccagaacacg tcgatggtcg aggcgttccg
     4561 caaggccgac tcggtgctcc tcaacgcggt ccagggcatc agcgacctga tgacggtccc
     4621 gggcctcatc aacgtcgact tcgccgacgt ccgcacgatc atggcaggca tgggccgcgc
     4681 gctcatgggc accggcatcg gctcgggcaa gaagcgcgcg accgaggcgg ccgagacagc
     4741 gatcagctcg ccgctcctcg aggacgtctc gatcgaaggc gcgaccggca tcctgatcaa
     4801 catcaccggc ggtcccgacc tcacgctgca cgaggtcaac gaggcgtcgt cgctgatcca
     4861 gcaggccgcg cacgaagacg cgaacatcat cttcggctcg gtcatcgatc cgaacctcag
     4921 cgacgaagtc cgcatcacgg tgatcgcgac cggcttcgat cgcggcgcga agaccgaagt
     4981 catcatcgag ccgagccgca gcgcgccgaa cacggttgcg cgcaagccgt cgcagacgat
     5041 gctgccgtac gacgtgtcga gccagatcac gaaggagtac ccgccggtcc ccgtcgctcc
     5101 gcgcatgccg gcgaaggtgc aggcgcagat catcgacgag cccgacgtcc agatcgaatg
     5161 ggcgacgccc gacctgaacg agatgagcga gatcgagcgc gcactctccg actacagcga
     5221 gccggtcccc gagggtgagc cctcgcagag gatggccacg ggctcgggcc cggcgacgcc
     5281 ctccgacggt aatccgctcg tccatcccga tcgcaagcgc gcgcagtcgc gcccgagcat
     5341 gaaggcggtg ctggccggcg agatcgacac cgagagcgag ctggatgtgc cgacgttcat
     5401 ccgccgccac aacacgagcc acggttagta gttagcctga tccttcccta ggagcagaag
     5461 gcccgcttcg ggccttttgt attttcggag tactcttcgc gcgtgctcac cgaatcggcc
     5521 gcgcgctgga tgctcgtgct ccacacgatc gtcggcgtcg ccgcggtcgg cgcagcgaca
     5581 cacctcgtgc tgtggcttcg caagtacgtg cgcgggcaga gcggccgccg gcgcgcggtg
     5641 ctgcgctttg cgtggctagt cctcgcgctg cagctgtcgg cgttcatcgc gggcaatgtc
     5701 atgtacccga cctacaaggt cgaggttcgc gcggcctacc tcgagaacgc gcccgcgatc
     5761 accgcgagcg aacaggcgaa gcagcgcgag ctcggcaagc tggccgcccg cgagcacgcc
     5821 gaagcgagcg aaccggcagc gaccgtcgag atcgtcaaac gcgcggcgca ggcagcgcgc
     5881 tggttcgacg tcaaagagca ttggatcgcg ctcggtctgt tcgtgtcagc cgcgctgatc
     5941 ctcgtacttt ctctgtggga tccgaagggg gaaggccgcg tgatcgctcc gatcgtgatg
     6001 agcctggccg tgatcgtcgc gggcaccgtc tggttcggcg caatcgtcgg cgtgctcacc
     6061 gcgtcgtggc gcgcggtcta gcggtcgtgt tacgaccttg cgcatgcgga tcttgcttgc
     6121 attgacggtg ttcgcgtttg cctgcggcgg cacgaagccg aagaccgaga gctcgatggt
     6181 caacgaaggt tcggaccaag cgccgacctg ctgctgcaag accatcccgc aggtcggtga
     6241 gaaggaaatc gtcccgaact acaacatgca aggacgcatg gagtgctcgg ccgcgaatgg
     6301 cgagtgcgtg gacgacgtcc agtgcaacaa gaccggcgaa ggcgggacgg aagttccggc
     6361 gagcaccggt actggtggtc ctggcgagcc tccgccgccg cccgtgctcg agccgtcgac
     6421 gtcctcgacg ccatgatcgc gtaagcgtaa gcggcaaacg ccgcgaccgg gtcccgcgaa
     6481 cccgcagcgg tcgtcgccct gcgattgcgc cggccccgat gaagtgctgt gaaacgaacg
     6541 atcggtcgca ccgtgctgga aagcgcgatc gtcgattcga cgaacgtccc gttcaaccga
     6601 cgtccgattc ggttgacgcc ccaatccggg aggcgcactc gccccctcgc tccgatcgga
     6661 tcggaacgca gagccgctta cgcttacgct tgcgcctacg tcagaacgta aacgccaggc
     6721 tcgactggaa gagcatcgag cgatcgatca ttcgatcctc gtccatcatc tcgaccggct
     6781 tgcatgactc gccgcgacag atcgagccga ccatctgctg gtgcggcgac atcgcgaccc
     6841 gccagccgag gaaccaaccg acgcgatgcg ggaagccgat cggctcctgc agccggtgat
     6901 cgagcgctat cccgaagccg accaggcctt cgggacgccg cgtgtcatcc ggaacgagac
     6961 gactcgcttc gtcgtaggcg ggcttgtacc agtggttcca cgcggtgcca gcgccaccct
     7021 ctacgtagat cgacagcagc gagttctggc cgacgatccg cgggccgagg cggatgacgt
     7081 cgaggcggcc gagcgccgag agcctctggc cggcgccgag cggcgtgtcg tcgggacctt
     7141 tgacctgaaa cgccaggtac gtgtactcgg cttcgatcgt gaagcggccg aatcgatagc
     7201 cgattgcggc gttaccgccg acggccgtga cgtcgaccgg ctcgtggtag aggaagccga
     7261 ccgcgctgcc aatccttgcc tctgacccgg tcgtgtgatg gggcgcgtag ggatcctcga
     7321 tgtagaagtc cgccttgtgc gtggcctcgt cggcccatgc gacacctccc cctgtgaatg
     7381 tcaggcacag gagcagccag cgagcgatcc ggaaccgacg gaagttcctc atctgcacct
     7441 cttctcgtcg aagagcgttg cagtcagcgc gccagtgaag cgtcgagggc gcgggccatc
     7501 acggcgacgg gggccggcgt cccagtccag atcgcaaacg cgcgtgcgcc ctggtggacg
     7561 agcatcgcgc gaccgtcgag cgtggaatga ccgaggctgc tcgcgcgttc gagcaggttc
     7621 gtccgccggt gatagaccag cgtcgagacc cacgctcccg gcggcaacgc gtcgagcggc
     7681 agcgcatcgg tgaacgctgc gtcggcctcc ggatccatcc ccgccgacgt gcagtcgacg
     7741 acgagatctg cacgtgcgaa acagtcacgc aggttctctt ctgtccaggg ccacgcttgc
     7801 gcccagctga cctcggcggg cttacgtgca actacttcga tcgctctcgc gctgcgaacg
     7861 ccatacgcga cagcgcgcgc cgcaccgccg gcaccgagca ggatgacgcg cttgccgcgc
     7921 agctcgcagc cagctgcgat cacagcgtcg agaaatccgt cgcagtcggt attgtggccg
     7981 actagctgat cgccgaactg caggcagttg accgcgccga tcgccttcgc ttcgggcgat
     8041 agctcgtcac acagctgcgc gaccgcgagc ttgtgcggga tcgtcacgct cgcgccgagc
     8101 gctttcatcg cacggagcgc ggcgacgacc gtcgcgagtc cgtcaggcgg cacgccgatc
     8161 ggcaccatca ccgcatcgat ctgatcggca gagaacgctg cgttcagcat cgtcgggctg
     8221 cgcgtgtgat cgaccggcca cccgagcacc gcagcgattc gtgtcgtgcc gctgatcgtc
     8281 atggctgcac cgccagctcg gcgccgtcgc ggcgcgcttg gtcgagcgca cgcgtcgcat
     8341 ccttcatcag cttcgcaaag ctgagtgctt ggcccggcgc accgccggcc accgcaccga
     8401 cgacgcgcgg cggaaacgac cgaccgccag cgaccactgg atcgccctcg gcgacggctg
     8461 cgatcacacg ccggccgaga ttggccgagc ctttgagatc tgtgtacggc agaagcacga
     8521 gaaa
//
LOCUS       DASUFX010000683        15563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_943790,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000683 DASUFX010000000
VERSION     DASUFX010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943790"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1627
                     /locus_tag="VFV99_27545"
     CDS             113..1627
                     /locus_tag="VFV99_27545"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HEX5063173.1"
                     /translation="MGVVFDAGTPEQAEQGGPIFAVARTPKGHRLRFGTPGPDLGKVR
                     VGDFVWVSSDPRVTRAGEKAAEAGRLPLGRIPIEIEVEGAVGTKLVVRASSARGRCNV
                     TSAADLAAARGAGITRELLLDKLGGLGGTPFHLGALDTSRLAAGLHLPVSELKDLRRS
                     IVMQLDAALARVEREVTADSVIESVRARITTTPPALDRLPVVVPLCRDDAQLDAVLDA
                     GAKEVELDWMELVGLARAVERARSRGARVSVATVRVQKPGEEKIDAHLARLEPDAVLV
                     RSWGSLAYFEDLRRTHVGTDRDAHVGSNRDQHGPNAPVLHGDFSLNVTSSITAGWVLG
                     HGLATITAAHDLDREQLLALLDAAPRGRVAVTVHHHIPTFHTEHCVYAHLLSTGRDYR
                     TCGRPCEQHKVALRDRVGLVHPVIVDVGCRNTVFNAQAQSAATLVPELLARGVQRFRV
                     ELVRESAEEARRVYTAYTQLVAGAITPADVVRVAAVHEQFGVTRGTMRTLTVLR"
     gene            1889..2749
                     /locus_tag="VFV99_27550"
     CDS             1889..2749
                     /locus_tag="VFV99_27550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063174.1"
                     /translation="MARRPSPPLMPGKPMQDANFPAPISVGIAAALATSTMMKTAVTA
                     SLLLVASTAFADTTTTTADTLVVVTPNAPVVVTQGAAPAPQPVYAAPGAAEMPPPPPA
                     YPAAPATANGAPQNEDWNNVSHINGIPVKVGERQDYLYKPKKFNISTNPFGYFFGYYD
                     IAGSMALGQNLAASVAITGWDESYSTGYQLSATLPLYFRKTFSGPFLEGGLLIRETKH
                     DYYYDCYDCSSGSTMDTDSWVGPQLLFGWHWTFDSGLNVAFAAGVAKQMGNTNQDTDG
                     NAYFRVGYAF"
     gene            complement(2836..3597)
                     /locus_tag="VFV99_27555"
     CDS             complement(2836..3597)
                     /locus_tag="VFV99_27555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063175.1"
                     /translation="MTEREIESLKFFADIESQTMYYFLEVAKLQVARDPELLTFLTMW
                     NYEEYFHSHAITRLMEECGVKLESAMDRSTEVRANARFKAKFEDFGQTLIAKAAPNHF
                     VSLWMFWGALQECLTMQAYEEVIRQTKNPVLAEMFRRIAKQERRHFAYYFGQARKRLE
                     GKPSHQRFCRFIAKHFYGPVGSGVKTDAQSAEIVARLFPNDRIIEVMSYIERRMAQLP
                     GMAGLDACTRWAAKIQPILPAELRATSAAVESIAA"
     gene            complement(3762..7361)
                     /locus_tag="VFV99_27560"
     CDS             complement(3762..7361)
                     /locus_tag="VFV99_27560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063176.1"
                     /translation="MEVADAAPVLGAGRFEVRGTLGAGGAGVVYRAFDRQLQREVALK
                     LLRSASGRDLYRFKQEFRALADIVHPNLVTLHELHAAEGDWYFTMELVEGVSFLDWVR
                     PSTTSGPTRTRQDILATLVHEVRLRGALVQLIDALIALHKANKLHRDLKPSNVLVTPQ
                     GRVALLDFGLVANLAEDNPEKLAVGTPVYMSPEQASDQPLTEASDWYSLGAMIYEALT
                     GRRPFEGESEQVMTRKQTELPLDPKQVAPATPPDLARLCMLLLQPQSSARPNGFAILG
                     MLGATPSPRTRDMVRTSVPATFVGRTRELDELRRALGDARRRGVAMMVKAKSGMGKTA
                     CVRKFLRGLGESVFVLEGRCFEREQVPFKMLDGIVDMLTNVIVALPPHEIEGLAPKEL
                     GSLVRLFPVMKRVKRFGELAPESPPPADPAELRRRGFQALRSLLGKLAKIRPVVMFVD
                     DAHWGDYDSSVFLNELIHSGEPPVLTILAHRPEDYFGVVAKLKHPQIGVRRGDVREIE
                     IKALDEGDAFTLVGQLAQDPARVDQIVRSGAGNPLILSELAQAPQLAANAKVDDLVRA
                     RVGRLTQDAQAMLAVSSVAAKPIPIDIAAHAAGIVSGHDAAAQLSAERLATLRRVNGQ
                     MILQPAHDHVRVAVLASLDIESKAGWNEALARAYEDIQGEAALDTQAVVEHWLAAGHP
                     ANAAHHAVSAATRAEEALAFRRAAELYEIALTYGPWDAAGQRDLLRAKAHALACAGQL
                     DEAATVYGHAAQILPDEESLECDRLRIEALLRRGRLDDALPAAEKILKQIGVRSPFGQ
                     SRTKLATQWMQQKLRSLDFVERDPSSMSRASLLHVDVLYSIVSGLAFADPALGRALQS
                     ELIRAAFEAGEPVRACLALAQEVCYAASSGSRNANAVAAVGARLRALAAQVDHPHITG
                     LTDTAIGIAAYMSGEWRDARTHLEAGLAVLRDHGAGVRWEIDIGETYWLATLYYLGEW
                     RDLVRQSQVLLRDAIERGDIVAQLGIRTGRCNLAWLIAGRLEEAHAQLQAAHASLPTG
                     FHLPHVLWVQAACNVDLYAGNIADAAKRLDETWPEIDRIGALRSQHLRVELLLLRARI
                     LLAQKKRTSEVARIADELTREGATWANGIGHVLRAATQRTVDDAIVSCEQAEEDFTTT
                     GMVGWLYVARLRRGQLEGGLGGAARAEAARDFLKDLGAAFPDRIANLLVPWHG"
     gene            complement(7374..8078)
                     /locus_tag="VFV99_27565"
     CDS             complement(7374..8078)
                     /locus_tag="VFV99_27565"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HEX5063177.1"
                     /translation="MTTLVYIGPTLPADEVRARLPSATVLPPVAVGDILKASRQRGVK
                     RIAIVDGYFERMAAVWHKEILVALERGIAVYGAASMGALRAAELAPWGMIGVGSIYRD
                     YARGVLVADDEVAVAHLPAEYGYRAVSDALVNIRYGLRAAAKAKVIPPALVEGLVDLA
                     RLRFYRERTWTQLVTDAGAAGMRVDSLTAWPKPDRKRKDARLLLRRLGTDRMVRPARI
                     RVPRTWALRQLESLML"
     gene            complement(8075..9358)
                     /locus_tag="VFV99_27570"
     CDS             complement(8075..9358)
                     /locus_tag="VFV99_27570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX5063178.1"
                     /translation="MLRFYDTVLDAPTKLRDGTHRAASLDATWKRFARVAPAAGITRI
                     ADITGLDTLGIPVFAAIRPMGLSLATQQGKGISPEAARVSALMESLETYSAEHIEREA
                     VRDSYRALSKRTNVVDVRRLPRALPAALPAERPGDKRRPADKRRRGDKRRRVAPTRRH
                     AMRRGGRLDLDARWRWVEGFDLTAGEPILVPREAVTLDTTFTKPPTFDISSNGLASGN
                     VLVEAIVHGLCEVLERDAESAWRRAGGDRRLVLDTITDPACRALIDRITATGARVFIW
                     DLAAESGIAAIGAAIMEDPNEPAWRTLGFYQGFGAHLLPEIAIARALTEAAQTRLTYI
                     AGARDDFFPFDYARATDPELLADLWQRLAAPCDEPVIFDDLPRRETRGLGEDLEALVG
                     LCEQVIVVDLTHRKLRVPVVKVIVPGRATDVEALG"
     gene            9387..9881
                     /locus_tag="VFV99_27575"
     CDS             9387..9881
                     /locus_tag="VFV99_27575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063179.1"
                     /translation="MRVIALCLALATVSACSTGGQYVSGGRGEGDLDRGETNGRMFDF
                     VSNMPEGDDWQIRVRDSSLWAAYGDGEKVKELGTANLTTKETDKLWKLVDAVEIPDRK
                     KGKRDEDEGYVQLRLREPGEEQHDIFEIYVSRADAADDEEVLSLAEYLQKLIEKHFKK
                     KPEF"
     gene            9881..10333
                     /locus_tag="VFV99_27580"
     CDS             9881..10333
                     /locus_tag="VFV99_27580"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HEX5063180.1"
                     /translation="MATTHGILISLSSKRLALIESEPDTLEDVVEARHEDAIPGLLDL
                     KTYWETLDMIMSGRGREEILGDALLARTGKNLEVDNAFEQAKLLSPQRVAEVSAKLGE
                     LTTATLKERAQQLGKTKDMDQLLALFKQVVALYADAAKAKQSMLAILI"
     gene            complement(10334..11626)
                     /locus_tag="VFV99_27585"
     CDS             complement(10334..11626)
                     /locus_tag="VFV99_27585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063181.1"
                     /translation="MARLSSAEVFTPTSFPFHTYVERPELHNERLLQEWLRSSTQIAS
                     VSGPSKAGKTVLIQRVVGEDNLCTISGAQVRTADQLWERVLDWYGEPHSTTATNADAT
                     TDTSAHEKSVTVGVPGTGAAGKHLSTHAQMGTETTSATVNRRGLAQVVQEIAHSPYTL
                     LLDDFHYIPPAVQADVAQQLKDASSRGVRMCVASVPVRADNLVRALPELRGRVLSVDL
                     DYWKQKDLLQIPKLGLPLLNLEVDEASLQMFAREAAGSPQLMQTICLWMCNHLGVRET
                     LDQPRSVKLDELGRKEILFLTSCTADFRSLVRAMISGPRARPGERRSYVHQDGRIADV
                     YLSIMRAVAMDPPRLSIDYADLQQRLEVLCKGASPEGSSLVNACARLGQIASGFPSLT
                     GPPFEWDEQAQMILMPDPYLLFYLRWSVALEREADALM"
     gene            11643..12242
                     /locus_tag="VFV99_27590"
     CDS             11643..12242
                     /locus_tag="VFV99_27590"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5063182.1"
                     /translation="MIELYGAPASSAGRTHLLLEEIGVPYNYHRVNLRDPATKAEFLK
                     MNPSGRVPFLLDGDVVLQESIAINFYLAEKYAPQLWSTNAATRAQIYMWSLWAITNLQ
                     PEVMRVARHTMFIPADARSAYEVETGKSNTQSLLDELERGLTGSYLVGGTLTVADINV
                     ASVVNLVGPFQAGTAGAKTKAWLDGLKARPAWQNVAKGG"
     gene            12385..13461
                     /locus_tag="VFV99_27595"
     CDS             12385..13461
                     /locus_tag="VFV99_27595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063183.1"
                     /translation="MERLWRSLEAHAQSSYFLSWAFIENWLAALPEDERPALAVIFDG
                     GQPTGAFFLAQRRLRRNLVMTSNALYFNATGAPKLDELAIEHNGMLAMPGSSRSLASV
                     LALLPGDWDELYLPALDRYAFDDLGGASSPLAEHYKVRIEREETAPFVDLDAVRAVEG
                     GYDALLPASTRTHIQRSRALLGNIDLEVAVDERQALDIYGELLRLHARRWAARGLRGA
                     FADPWFERFHRQLILNRLLYGDIQLMRIKAGSTTLGCLYNFVHRGRVVFYQSGLATLE
                     DTHIKPGYICHATAIEYNALAGHAFYDLLGGQAKYKENLATGLNRRVWLRVQRPLARF
                     SLEDGVKRWYELLVGDRDLALRPV"
     gene            complement(13458..14315)
                     /locus_tag="VFV99_27600"
     CDS             complement(13458..14315)
                     /locus_tag="VFV99_27600"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5063184.1"
                     /translation="MARASDRIAENVDGDFFVDSSCIDCDLCRQLAPATFARTRRHAQ
                     SFVQHQPADASEEHRALMALVTCPTSSIGTVRKHDSKAAARAFPELVDGDVYFCGWAA
                     ESSYGAASWLIKRPEGNVLIDSPRAASTLMDRLDELGGVRTMFLTHRDDVADHQKFHD
                     RFGCERVVHRADSVRGAEQVIDGVAPVRLADDLLVVPVPGHTRGSCVLIYRETYAFTG
                     DHVWATDEQNGLEAGEDVCWYSWPELKRSMARLAEHRFTWVLPGHGRRFHAANPETMR
                     AELLRLAAS"
     gene            complement(14362..15162)
                     /locus_tag="VFV99_27605"
     CDS             complement(14362..15162)
                     /locus_tag="VFV99_27605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063185.1"
                     /translation="MSERNPQAKQMADESMVRNLTAQADAIWPQEEPIIRGHGSPRDI
                     LDVGCGTGEISSRLATLFPTAQVTGVDLIEPHLALARSRYAAFGDRLRFQQADAFELP
                     FEARSFDLVVCRHMLQAVPTPERVIAEMVRVTRPGGRVHLLVEDYDMIHASGTGDVSS
                     FWHVAPRVFGGATGTDLHIGRNAIGHLRRLAVDDIHTHYAIVDTERVPRATFATIFEA
                     WRDGYANDIAGYLGWSPADVRAQFDATIECVRTGYAVWFVPIVTARVR"
     gene            15188..>15563
                     /locus_tag="VFV99_27610"
     CDS             15188..>15563
                     /locus_tag="VFV99_27610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063186.1"
                     /translation="MRAAVGAACAVLIGGCLIVRADEDIIEPSCPTTSPQLLAEASGP
                     FVVADAIYFIGANGVLARMPINGGVVSELTTEHIGANVIASDATDLYWTTGETIIRKP
                     LDGGAAYEIASGYVNVTKILVED"
BASE COUNT         2615 a         5215 c         5121 g         2612 t
ORIGIN      
        1 ggtccgatcg ccagacgctg cggtccaatc gccagacact gcggtccgat cgccagacac
       61 ggcagtccgg tcgccagaca agatggtccg tctcgcggac gttcgagcgg gcatgggcgt
      121 tgtgttcgat gcgggaaccc ccgaacaagc cgaacaaggc gggccgatct tcgccgtcgc
      181 acggacgccg aagggccatc gcctgcgctt cggcacgccg ggcccagatc tcgggaaggt
      241 gcgcgtcggc gatttcgtgt gggtcagctc cgatccaaga gtgacgcgcg ccggcgagaa
      301 ggcggcggaa gccggtcgac tgccgctcgg tcggatcccg atcgagatcg aagtcgaagg
      361 cgcggtgggc acgaagctcg tggttcgcgc gtcctcagcg cgcggacgtt gcaacgtgac
      421 gagtgccgcg gaccttgccg ccgcgcgcgg tgcaggcatc acgcgcgagc ttctgctcga
      481 caagctcggt ggactcggcg gcacgccgtt tcatctcggc gcgctcgaca cgagtcggct
      541 cgccgccggc ctgcatctgc cggtatcgga gctcaaagac ctgcgccggt cgatcgtgat
      601 gcagctcgat gccgcgctcg cgcgtgtcga acgagaggtc actgcggact cggtgatcga
      661 atcagtgcgt gcgcgaatca cgacaacgcc acctgccctc gatcggcttc cggtggttgt
      721 cccgctctgt cgtgatgacg cgcagctcga cgccgtgctc gatgctggcg caaaggaagt
      781 cgagctcgac tggatggagc tggtcgggct cgcgcgtgca gtagagcgag cgcgatcgcg
      841 tggcgcgcgt gtctcggtcg cgactgtgcg cgtgcagaag ccgggtgaag agaagatcga
      901 cgcgcatctc gcgcggctgg agcccgatgc ggtgctcgtc cgcagctggg gctcgctcgc
      961 gtacttcgag gatctgcgtc gtacgcacgt gggtacggac cgcgatgccc acgtgggttc
     1021 gaatcgcgat cagcacgggc cgaacgcgcc cgtgctgcac ggcgacttct cgctcaacgt
     1081 cacgagctcg atcacggccg gctgggtcct tggccatggc ctcgcgacga tcactgccgc
     1141 ccacgatctc gatcgcgagc agctgctcgc gctcctcgac gcagcaccgc gcggccgcgt
     1201 cgccgtcacc gtgcaccacc acattccgac gttccacacc gagcactgtg tctacgcgca
     1261 tctgctctcg acgggccgcg actaccgcac ctgcggtcgg ccgtgcgagc agcacaaggt
     1321 cgcgttgcgc gatcgcgtgg ggctcgtgca tccggtgatc gtcgatgtcg gttgccgcaa
     1381 caccgtcttc aacgcgcagg cgcagagcgc cgcgacgctg gtgcccgagc tactcgcgcg
     1441 cggcgtgcag cggttccgcg tcgagctcgt gcgcgagagc gccgaagaag cgcgccgggt
     1501 ctacacggcg tacacgcagc tcgtcgccgg cgcgatcacg ccggccgatg tcgttcgcgt
     1561 cgccgccgtg cacgagcagt tcggcgttac acgaggcacg atgcgaacgc tgaccgtgct
     1621 gcgttagtcg gtcgtcgtcc gcggatacgg ccacagccgc atgcgcaccc catccacgag
     1681 ccacgtgtgc tcgtactacg acggcagttg gcgccactgg tgcagcagct gtgttcagat
     1741 gttgcaggcg atgccgaccg acgctgcgcg ccatctggcg tgcgccccga catcgttgtc
     1801 agagccacgg aaatctgagg gatcgcggca atttcgcgga tgacctaatc cgcgacaggc
     1861 gtgtcaggaa tctcgcggtt tttgtcaaat ggcccgtcgg ccttcaccac cgctgatgcc
     1921 tggcaaaccg atgcaggacg cgaacttccc cgcaccgatc tcggttggca tcgcggctgc
     1981 tctagcgacc tccaccatga tgaagacggc cgtcaccgcc tcgctcctcc ttgtcgcctc
     2041 gactgcgttt gcggacacca ccaccacgac tgccgacacg ctcgtcgttg tcacgcccaa
     2101 cgcccccgtg gtcgtgacgc aaggcgcggc gcctgctccg cagccggtct atgcagcacc
     2161 gggtgctgcc gagatgccgc cgccgcctcc ggcctacccg gccgcgccgg cgaccgcgaa
     2221 cggcgcgccg caaaacgaag actggaacaa cgtctcgcac atcaacggca tcccggtgaa
     2281 ggtcggcgag cgccaggact acctgtacaa gccgaagaag ttcaacatct cgacgaaccc
     2341 gttcggctac ttcttcggtt actacgacat cgccggcagc atggcgctcg gccagaacct
     2401 cgcggcgtct gtcgcgatca ctggctggga cgagtcgtac tcgaccggtt atcagctcag
     2461 cgcgacgctg ccgctgtact tccgcaagac gttctcgggt ccgttcctcg agggtggtct
     2521 actgatccgc gagacgaagc acgactacta ctacgactgc tatgactgct cgtcgggctc
     2581 gacgatggac accgactcgt gggtgggtcc gcagctgctg ttcggctggc actggacgtt
     2641 cgactcgggc ctgaacgtcg cgtttgcggc gggtgttgcg aagcagatgg gcaacaccaa
     2701 ccaggacacc gatggcaacg cgtacttccg cgttggttac gcgttctaaa ccacgcgcac
     2761 ggtgacggcc ggccgcgaat cgcgaggagc gacgcggccg gtttgccgtt tcggctacag
     2821 caacgcgcgg ttcggttagg ccgcgatgct ctcgaccgcc gcggacgtcg cgcgcagctc
     2881 ggccggcagt atcggctgga tcttcgctgc ccagcgcgtg caggcatcga ggcccgccat
     2941 gcccggcagc tgcgccatgc gccgctcgat gtagctcatc acttcgatga tgcggtcgtt
     3001 cgggaacagg cgcgcaacga tctcggcgct ctgcgcgtcc gtcttcacgc cactgccgac
     3061 cggcccgtag aagtgcttcg cgatgaagcg acagaagcgc tgatggctcg gcttaccctc
     3121 gagccgcttg cgcgcttgac cgaagtagta cgcgaagtga cggcgctctt gctttgcaat
     3181 gcggcgaaac atctccgcga gcaccgggtt cttcgtctga cgaatgacct cttcgtacgc
     3241 ctgcatcgtc aggcactctt gcagcgcgcc ccagaacatc cacagcgaga cgaagtgatt
     3301 cggcgcagcc ttcgcaatca gcgtctgccc gaagtcttcg aacttcgctt tgaaccgtgc
     3361 gttcgcccga acctcagtac tgcgatccat cgcgctctcg agcttcacgc cgcactcttc
     3421 catgaggcgc gtgatcgcgt gcgagtggaa atactcctcg tagttccaca tcgtgaggaa
     3481 cgtgagtagc tcggggtcgc gcgcgacttg cagcttcgcg acctcgagga agtaatacat
     3541 ggtttgactt tcgatatcgg cgaagaactt gaggctctcg atctcccgct ccgtcaaccc
     3601 atgcttgcgc gccagccccc agtctagatc gtcgatctca accctgtcgg ataccgcgat
     3661 gtgctgagcg atgtctgaca cgtttccctc ttgctccctc tacgaaccac cgaccgttcc
     3721 ggatcagcgt tcatgcagcc taatgcaaaa tgcccggacg atcatccgtg ccagggcacg
     3781 agaagattgg cgattcggtc agggaacgcc gcacccaggt ccttcaggaa atctcgcgcg
     3841 gcttccgcgc gcgccgcgcc gccaagtccg ccttccaatt ggccgcgccg cagccgggca
     3901 acgtacagcc atccgaccat tccggtcgtc gtgaagtcct cttcagcctg ctcgcagctg
     3961 acgatcgcgt catcgaccgt gcgttgggtc gccgcacgca agacgtggcc aatgccgttg
     4021 gcccacgtgg cgccctcgcg cgtcagctcg tctgcgatcc gcgcgacttc ggaggtgcgt
     4081 ttcttctgcg cgagcaggat gcgcgcgcga agcaagagca gctcgacgcg caggtgttgg
     4141 ctgcgtagcg cgccgatgcg atcgatctcc ggccaggtct cgtcgaggcg cttcgccgca
     4201 tcggcgatgt tgccggcgta gagatcgacg ttgcacgccg cctgcaccca aagcacgtgc
     4261 ggcagatgga agcccgttgg caacgaggcg tgtgccgcct ggagctgcgc atgagcctcc
     4321 tcgagtcggc ccgcgatgag ccacgcgagg ttgcagcggc cagtccgaat gccgagctga
     4381 gcgacgatgt cgccgcgctc gatcgcgtcg cgcagcagca cctggctctg gcggaccagg
     4441 tcgcgccact cgccgaggta atagagcgtc gcgagccagt acgtctcgcc gatgtcgatc
     4501 tcccagcgca cgccggcacc gtgatcgcgc agcacggcga ggccggcctc gagatgcgtg
     4561 cgggcgtcgc gccactcgcc actcatgtac gcggcgatgc cgatcgcggt gtcggtcagg
     4621 cccgtgatgt gcggatgatc gacctgcgcc gcgagcgcac gcagccgcgc accgacggcg
     4681 gcgaccgcat tggcgttgcg actgccactg ctcgccgcgt agcagacctc ttgcgcgagc
     4741 gcgaggcacg cgcggaccgg ctcaccggcc tcgaacgctg cgcgtatcag ctccgattgc
     4801 aacgcgcggc caagcgccgg gtcggcgaat gcgagaccgg agacaatcga atacagcacg
     4861 tcgacgtgca gcagcgaggc acgtgacatc gacgacggat cgcgctcgac gaaatcgagg
     4921 ctgcgcagct tctgttgcat ccactgcgtc gcgagcttgg tgcgcgattg accgaacgga
     4981 ctgcgcacgc cgatctgctt caggatcttc tcggcggccg ggagcgcatc gtcgagtcgg
     5041 ccgcggcgca gcagtgcctc gatgcgcagg cgatcgcact cgagcgattc ctcgtcgggc
     5101 aggatttgcg cggcgtgacc ataaacggtt gccgcttcgt cgagctggcc ggcgcaggcg
     5161 agcgcatgtg ccttcgcgcg gagcagatcg cgctggccgg ccgcgtccca cggtccgtag
     5221 gtcagcgcga tctcgtagag ctcggcggcg cggcggaacg cgagtgcttc ctcggcgcgg
     5281 gttgccgcgg agaccgcgtg atgcgcagcg tttgcaggat gacccgctgc gagccagtgt
     5341 tcgacgacgg cctgggtgtc gagcgccgct tcgccctgaa tgtcctcgta ggctcgcgcg
     5401 agcgcttcgt tccagccggc cttcgattcg atgtcgaggc tcgcgagcac cgcgacgcgg
     5461 acgtgatcgt gggcgggctg caggatcatc tgcccgttca cccggcgcag cgtcgcgagg
     5521 cgctccgccg acagctgcgc ggcagcatcg tggccgctga cgatgcccgc tgcgtgcgca
     5581 gcgatgtcga tcggaatcgg cttggccgcg acactcgaca cggcgagcat tgcctgcgcg
     5641 tcctgcgtga gccggccgac acgcgcgcgc acgagatcgt cgaccttcgc gttcgccgcg
     5701 agctgcggcg cctgggcgag ctcgctcagg atcagcggat tgccggcgcc cgagcgcacg
     5761 atctgatcga cgcgcgcggg atcctgggcg agctggccga cgagcgtgaa tgcgtctcct
     5821 tcgtcgagcg ctttgatctc gatctcgcgg acgtcgccgc gccgcacgcc gatctgcgga
     5881 tgcttcagct tggcgacgac accgaagtag tcctcggggc gatgcgcgag gatcgtgagc
     5941 accggcggct cgccggaatg aatcagctcg ttcaagaaca ccgagctgtc gtaatcgccc
     6001 cagtgcgcgt cgtcgacgaa catgaccacg ggccggatct tcgccagctt gccgagcagc
     6061 gagcgcagcg cctgaaagcc gcggcgacgc agctccgccg ggtcggcagg cggcggcgat
     6121 tccggcgcga gctcgccgaa tcgcttcacg cgcttcatca ccggaaacaa gcggacgagc
     6181 gagccaagct ccttcggcgc gagcccttcg atctcgtgcg gcggcagcgc gacgatcacg
     6241 ttcgtcagca tgtcgacgat gccgtcgagc atcttgaacg gcacttgctc gcgctcgaag
     6301 cagcggccct cgagcacgaa cacgctctcg ccgagcccgc gcaggaactt gcggacgcat
     6361 gcggtcttgc ccatgcccga cttggctttg accatcatcg cgacgccgcg ccggcgtgca
     6421 tcaccgagcg cacgccgcag ctcgtcgagc tcacgcgtgc gaccgacgaa cgttgccgga
     6481 accgacgtgc gcaccatgtc gcgcgtgcgt ggcgacggcg tcgcgccgag catgccgaga
     6541 atcgcgaagc cgttcggtcg cgcgctcgat tgcggctgca gcagcagcat gcagagccgc
     6601 gcgagatccg gcggcgtcgc cggcgcgacc tgctttggat cgagcggcag ctcggtctgc
     6661 ttgcgcgtca tcacctgctc ggactcgcct tcgaacggcc gccggcccgt cagtgcctcg
     6721 tagatcatcg cgccgagcga gtaccagtcg ctcgcttcgg tcagcggctg gtccgacgcc
     6781 tgctcgggag acatgtagac cggcgtgccg accgcgagct tctccgggtt gtcttcggcg
     6841 aggttcgcga cgaggccgaa gtcgagcaac gcgacgcggc cctgcggcgt gaccagcacg
     6901 ttcgacggct tcagatcgcg atgcagcttg ttcgccttgt gcagcgcgat cagcgcgtcg
     6961 atcagctgga cgagcgcccc gcgcaggcgg acctcgtgga ccagcgtcgc gaggatgtcc
     7021 tgccgcgtcc gcgtcgggcc gctcgtcgtc gacggtcgaa cccagtcgag aaacgacacg
     7081 ccctcgacga gctccatcgt gaagtaccag tcaccctcgg cggcatgcag ctcgtgcaac
     7141 gtgacgaggt tcgggtggac gatgtcggcg agcgcgcgga actcctgctt gaaccgatac
     7201 aggtcgcggc ccgacgccga ccgcagcagc ttgagcgcga cctcgcgctg cagctggcga
     7261 tcgaacgcgc gatacacgac gcccgcgcca ccagcgccga gcgtgccacg cacctcgaag
     7321 cggccggcgc ctaggactgg cgcagcgtcg gcgacttcca tcgtctaccc agtctacagc
     7381 atcagcgatt cgagctggcg taacgcccag gtacgaggca cgcgaatccg cgcgggtcgc
     7441 accatccgat cggttccgag cctacgcagg agcaaccgcg cgtcctttcg tttgcggtcc
     7501 ggtttgggcc acgcggtgag gctgtcgacg cgcatgccag cggcgccagc gtcggtgacg
     7561 agctgcgtcc aggtccgttc gcgatagaac cgcaagcgcg cgagatcaac gagtccttcg
     7621 acgagcgcag gcggaatcac cttcgccttt gccgcagcgc ggaggccgta gcggatgttg
     7681 accagcgcgt cggagacggc ccggtacccg tactcggccg gcaggtgcgc caccgcgacc
     7741 tcgtcgtcgg caacgagcac accgcgcgcg tagtcgcgat agatcgagcc gacgccgatc
     7801 atgccccacg gcgcgagctc ggcggcgcgc aacgcgccca tgctcgctgc accgtagacc
     7861 gcgatgccac gctcgagcgc gacgaggatc tccttgtgcc agaccgctgc catgcgctcg
     7921 aaatagccgt cgacgatcgc gatgcgcttc acgccgcgct gtcgcgacgc cttcaagatg
     7981 tcgccgaccg cgacgggagg cagcacggtc gcactcggca aacgcgcgcg gacctcgtcg
     8041 gcgggcagcg tcggtccgat gtagacgagc gtcgtcaccc gagcgcctcc acgtcggtgg
     8101 cacgaccggg cacgatgact ttgacgaccg gcacgcgcag cttgcggtgc gtgagatcga
     8161 cgacgatcac ctgctcgcag agcccgacga gcgcctcgag gtcctcgcca agcccgcgcg
     8221 tctcacggcg cggcaggtcg tcgaatatga cgggctcgtc gcacggtgcc gcaagccgct
     8281 gccacaggtc ggcgagcagc tcggggtcgg tcgcgcgcgc gtagtcgaac ggaaagaagt
     8341 catcgcgtgc accggcgatg tacgtcagcc gggtttgcgc cgcctcggta agtgcgcgag
     8401 cgatcgcgat ctcgggaagc agatgcgcgc cgaagccctg gtagaagccg agcgtgcgcc
     8461 aggcgggctc gttcggatcc tccatgatcg ctgcaccgat cgccgcgatg ccggactccg
     8521 ccgcgagatc ccagatgaac acgcgtgcgc cggtcgcggt gatccgatcg atcagcgcgc
     8581 ggcacgcggg gtcggtgatc gtatcgagca cgaggcgacg gtcgccgcct gccctgcgcc
     8641 atgcgctctc ggcatcgcgc tcgagcactt cgcacaagcc gtgcacgatg gcttcgacga
     8701 gcacgttgcc cgacgcgagc ccgttcgacg agatatcgaa cgtcggcggc ttggtaaatg
     8761 tcgtatcgag cgtgaccgcc tcgcgtggaa cgaggatcgg ctcgcccgcc gtcaggtcga
     8821 atccctcgac ccacctccag cgcgcatcga gatccaagcg ccctccccgt cgcatcgcat
     8881 gtcgtctggt cggcgcgaca cggcggcgct tgtcgccgcg gcggcgcttg tcggcggggc
     8941 ggcgcttgtc gccggggcgc tccgccggca gcgccgctgg caatgcgcgc ggtaggcgtc
     9001 gcacgtcgac aacgttcgtg cgcttgctca gcgctcgata ggaatctcgc acggcctcgc
     9061 gctcgatgtg ctccgcgctg taggtctcca ggctctccat caatgccgag acccgcgccg
     9121 cctccggcga tatccctttg ccctgctgcg tcgccagcga tagccccatc ggccggatcg
     9181 ccgcgaacac cgggatcccg agcgtatcga ggccggtgat gtccgcgatc cgggtgatcc
     9241 ccgcggcggg cgcaacccgc gcaaatcgct tccaggtcgc gtccaggctc gcggcgcggt
     9301 gggtgccgtc acgaagcttc gtcggcgcgt cgagcaccgt gtcgtagaag cgaagcatcg
     9361 gcgtggccgg atgctataac ccaggcgtgc gggtcatcgc gctctgtttg gcgctcgcga
     9421 cagttagtgc gtgctcgact ggtggccagt acgtctccgg tggccgcggc gagggcgacc
     9481 tcgaccgtgg tgaaacgaac ggccggatgt tcgatttcgt gtcgaatatg ccagagggcg
     9541 acgactggca gattcgcgtt cgcgacagct cgctctgggc cgcctacgga gatggcgaga
     9601 aggtcaaaga gctcggcacc gcgaacctga ctacgaagga aaccgacaag ctgtggaagc
     9661 tcgtcgatgc agtcgagatt cccgaccgca agaaaggcaa gcgcgacgaa gacgagggct
     9721 acgtccagct ccgcttgcgc gagccgggcg aggagcagca cgacatcttc gagatctatg
     9781 tttcccgggc cgatgcagcg gacgacgagg aagtgctatc gctagcggag tacctgcaga
     9841 agctgataga gaagcacttc aagaagaaac cggaattctg atggcgacca cgcacggcat
     9901 cctgatctcg ctgtcctcga aacggctcgc gttgatcgag tccgagcccg acacgctcga
     9961 agacgtcgtc gaagcgcgcc acgaggacgc aattcccggc ctgctcgatc tcaagaccta
    10021 ctgggaaacg ctcgacatga tcatgtcggg acgcggccgc gaggagatcc tcggcgacgc
    10081 actactcgcg cgcaccggca agaacctcga ggtcgacaac gcgttcgagc aggcaaagct
    10141 gctctcgccg cagcgggtcg ctgaggtctc cgcgaagctc ggcgagctca cgacggcgac
    10201 gctcaaggag cgcgcccagc agctcggcaa gacgaaagac atggatcagc tgctcgcgct
    10261 gttcaagcag gtcgtcgcgc tctacgccga cgccgcgaag gcgaagcaat cgatgctcgc
    10321 gatcctcatc tgatcacatc agcgcgtcgg cctcgcgctc gagcgcgacc gaccagcgca
    10381 gatagaacag caggtacgga tccggcatca ggatcatctg cgcctgctcg tcccactcga
    10441 acggcgggcc agtcagcgac ggaaatccgc tcgcgatctg gccgagccgc gcacatgcgt
    10501 tgacgaggct cgaaccctcg ggcgatgccc ctttgcagag gacctcgagc cgctgctgca
    10561 gatcggcgta gtcgatcgac agccgcggtg gatccatcgc gaccgcgcgc atgatcgaga
    10621 gatagacgtc ggcgatccgc ccgtcttgat gcacgtacga gcggcgctcc cccggccggg
    10681 cccgcggtcc tgagatcatc gcgcgcacga gcgagcggaa atcggccgtg cacgacgtca
    10741 gaaataggat ctccttgcgc cccagctcgt cgagcttcac cgaccgcggc tggtcgagcg
    10801 tctcgcggac gccgaggtgg ttgcacatcc acaggcagat cgtctgcatc agctgcggtg
    10861 agccagcggc ctcgcgcgca aacatctgca gcgatgcctc gtcgacctcg aggttgagaa
    10921 gtggcagccc gagcttcggg atctggagca gatccttctg cttccagtaa tcgagatcga
    10981 ccgatagcac gcggccgcgc aactcgggta acgcgcggac gaggttgtcc gcacgcaccg
    11041 gcaccgacgc gacgcacatg cgcacgccac ggctcgatgc gtccttgagt tgctgggcaa
    11101 cgtcggcctg caccgcgggc gggatgtagt ggaagtcgtc gagcaggagc gtgtacggcg
    11161 agtgcgcgat ttcctgcacg acctgcgcta agccgcggcg gttcacggtc gccgacgtcg
    11221 tctcggtgcc catctgcgcg tgcgtcgaca ggtgcttgcc agccgcgccg gtgcccggca
    11281 caccgacggt gacgctcttc tcgtgcgcgc tcgtatcggt cgtcgcgtcg gcgttcgtcg
    11341 cggtcgtcga gtgcggctcg ccgtaccagt cgagcacgcg ctcccagagc tgatcggccg
    11401 tacgcacctg tgcaccgctt atcgtgcaca agttgtcttc gccgacgaca cgctggatca
    11461 ataccgtctt gccggccttc gacggacccg ataccgaggc gatctgcgta gaagaccgca
    11521 gccattcttg cagcagccgt tcgttgtgca attcggggcg ctcgacgtac gtgtgaaacg
    11581 gaaacgaggt cggtgtgaac acctcggcag aggacagccg cgccatggca ggatgatgcc
    11641 ccatgatcga gctctatggt gccccggcgt cgagcgcagg tcgcacgcat ctcctactcg
    11701 aggagatcgg cgtgccgtac aactaccacc gagtcaacct tcgcgatccg gcgacgaagg
    11761 ccgagtttct gaagatgaac ccgtcgggcc gcgttccgtt cttgctcgac ggtgacgtgg
    11821 tgctgcagga atcgatcgcg atcaacttct acctcgcgga gaagtatgca ccgcagctgt
    11881 ggtcgaccaa cgccgccacg cgcgcgcaga tctatatgtg gtcgctgtgg gcgatcacga
    11941 acttgcaacc cgaggtgatg cgcgtcgcgc gacacacgat gttcatcccg gccgacgcgc
    12001 gcagcgcgta cgaagtcgag accggcaagt cgaacacgca gtcgctgcta gacgagctcg
    12061 agcgtgggct cacgggcagc tatctcgtcg gcggcacgct cacggtcgcc gacatcaacg
    12121 tggcgtcggt cgtgaacctc gtcggtccgt tccaggccgg cacggccggc gcgaagacga
    12181 aagcgtggct cgacggcctc aaggcacggc cggcctggca gaacgtcgcg aaaggcggtt
    12241 gatcgcggtt ccgtgcggcg tggcagcggc gcacgcaggc gcacccgatc gcgcacaact
    12301 gcctgacggc acatgccttg ctgattgctt cggggcatga gcgatagccg cggcctcgag
    12361 ttggtcgatg cgcgtgacgg acacgtggag cgtctttggc gctcgctcga agcgcacgcg
    12421 caatcctcct attttctctc atgggcgttt atcgagaact ggctcgccgc gttgcctgaa
    12481 gacgagcgcc ctgccctcgc cgtgatcttc gatggcggtc agcccacggg cgcgttcttc
    12541 ctcgcgcagc gacgactccg ccgcaatctg gtgatgacga gcaatgcgct gtacttcaat
    12601 gcgaccggtg cgcccaagct cgacgagctt gcgatcgagc acaacggaat gctcgcgatg
    12661 ccaggctcta gccgctcact cgcgagtgtg ctcgcgctac tgccaggcga ttgggacgag
    12721 ctgtatctgc cggcgctcga tcgctacgcg ttcgacgatc ttggcggtgc ctcgtcaccg
    12781 ctcgccgaac actacaaggt acggatcgag cgcgaggaga ccgcgccgtt tgtcgatctc
    12841 gatgcggtac gcgcggtcga gggcggttac gacgcgttgt tgccggcaag cacgcgcacg
    12901 catatccagc gctctcgcgc gctcctcggt aacatcgacc tcgaggtcgc cgtggacgag
    12961 cgccaggcgc tcgacatcta cggcgagctg ttgcggctcc acgcgcgacg ctgggcagcg
    13021 cgcgggttgc gcggcgcgtt tgccgatccg tggttcgagc ggtttcaccg ccagctgatc
    13081 ctcaatcgcc tgctttacgg cgacatccag ctgatgcgaa tcaaggccgg ctcgacgact
    13141 ctcggctgcc tctacaactt cgtgcatcgc ggccgcgtcg tgttctacca gtctggtctc
    13201 gcaacgctcg aggacacgca catcaagccg ggctacatct gtcacgcgac tgcgatcgaa
    13261 tacaacgcgc tcgccggtca cgcgttctac gacctgctcg gcgggcaagc gaagtacaag
    13321 gagaacctcg cgacggggct caatcgtcgc gtgtggctac gcgtgcaacg gccgctcgcc
    13381 cggttctcgc tcgaggatgg cgtgaagcgc tggtacgagc tgctcgtcgg cgatcgcgat
    13441 ctcgcgctgc gccccgtcta actcgctgcg agtcgcagga gctcggcgcg catcgtctcg
    13501 gggttggccg cgtgaaagcg ccggccgtgt ccgggtagca cccacgtgaa tcggtgctcg
    13561 gcgagccgcg ccatcgatcg cttgagctcc ggccacgagt accagcacac gtcttcgccg
    13621 gcttcgaggc cattctgttc gtcggtcgcc cacacgtggt cgccggtgaa tgcgtacgtc
    13681 tcgcgataga tgaggacgca gctgccacgc gtatgtcccg gtacggggac aaccagcaga
    13741 tcatcggcga gccggactgg cgcgacgccg tcgatcacct gctcggcgcc gcgcaccgaa
    13801 tccgcacgat ggaccacgcg ctcgcagccg aatcggtcat gaaatttctg gtgatcagcg
    13861 acgtcgtcgc gatgcgtgag gaacatcgtc cgcacaccgc cgagctcgtc gagccggtcc
    13921 atgagcgtgc ttgccgcgcg cggagagtcg atcagcacgt tgccctcggg gcgcttgatc
    13981 agccacgacg ccgcaccata cgagctctcg gcggcccagc cgcagaagta gacgtcgccg
    14041 tcgacgagct cggggaacgc tcgcgctgct gccttgctgt cgtgcttgcg caccgtgccg
    14101 atcgagctcg tggggcacgt gacaagcgcc atgagcgcgc gatgctcctc gctggcatcg
    14161 gcgggctggt gctgcacgaa cgactgcgcg tggcgccgcg tgcgcgcgaa cgtggcgggc
    14221 gcgagctgac ggcatagatc gcaatcgatg cacgagctgt cgacgaagaa gtcgccgtcg
    14281 acattctcgg cgatccgatc gctggcgcgg gccatcacga tagcttgacg cgatttcggc
    14341 gcaacgccta ccgcttgaag ctcagcgtac gcgggcggtc acgatgggta cgaaccacac
    14401 ggcgtacccg gtgcgcacgc actcgatcgt cgcgtcgaat tgcgctcgga cgtcggccgg
    14461 tgaccagccg aggtatccag cgatgtcgtt cgcatagcca tctcgccacg cctcgaagat
    14521 cgtggcaaac gtcgcgcgcg ggactcgctc ggtatcgacg atcgcgtagt gggtgtgaat
    14581 gtcgtcgacg gcaagtcgcc gcagatggcc gatcgcattg cggccgatgt gcagatcggt
    14641 gccggtcgca ccaccgaaca cccgcggtgc gacatgccag aacgacgaca cgtcaccggt
    14701 gcccgaggcg tggatcatgt cgtagtcctc gacgagcaga tggacgcgcc cgccaggccg
    14761 tgtgacgcgc accatctctg cgatcacccg ctcgggcgtc ggtaccgctt gcaacatgtg
    14821 gcggcagacg acgaggtcga agctgcgcgc ttcgaacggc agctcgaatg cgtccgcttg
    14881 ctggaagcgc agccggtcac cgaatgccgc atagcgagac cgcgcgagtg cgaggtgtgg
    14941 ctcgatcaga tcgacaccgg tgacctgcgc cgtcgggaaa agtgtggcaa gtcgcgagct
    15001 gatttcgccg gtgccacagc cgacgtcgag gatgtctcgc ggactgccat ggccgcggat
    15061 gatcggctct tcttgaggcc agatcgcatc ggcctgggcc gtgaggttgc ggaccatcga
    15121 ctcgtctgcc atctgcttcg cttgcggatt gcgctcgctc atcgcgccaa tagcttactc
    15181 ttggagcatg cgagcggccg tcggcgcagc ctgtgctgta ttgatcggcg gctgtctgat
    15241 cgtgcgtgcg gatgaggaca tcatcgagcc gtcctgtccc accacgtcac cacaactgct
    15301 tgccgaagcc agcggcccgt tcgtggttgc ggatgcgatc tatttcatcg gcgccaacgg
    15361 tgtcctcgcg cgcatgccga tcaatggcgg cgtggtttcg gagctcacga ccgaacacat
    15421 aggcgcaaat gtgattgcca gtgacgcgac ggatctgtac tggaccaccg gcgagacgat
    15481 catccgcaag ccgctcgacg gcggcgccgc ttacgagatc gcgagcggct acgtcaacgt
    15541 gaccaagatc ctcgtcgagg aca
//
LOCUS       DASUFX010000684         8972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1777315, whole genome shotgun sequence.
ACCESSION   DASUFX010000684 DASUFX010000000
VERSION     DASUFX010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777315"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFV99_27615"
     CDS             <1..807
                     /locus_tag="VFV99_27615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063187.1"
                     /translation="VALVEPADGKALVELEQRLGAKITPYVVPELRALYYLEKHFGTP
                     RRARFSRSARPGTDPGELIGDDRRKTQPAGGIAMPPKLTLEPRRRRPSQAPMTQTVPT
                     TLSYGAACERIDTASNREQIADTFIDYARGRCDVLVVFLVRDGNALGWRGYVAPPNQA
                     TKPIEEISLPLGGASALQSAHDAGQTYVGQPPSPAKPIEGTLWTALGTEPKPIEVLVV
                     PILVKQRAVNLIYTHTLGGTPPQSLITELQDLAARAQTSYMRLIRQTRGS"
     gene            complement(1362..1814)
                     /locus_tag="VFV99_27620"
     CDS             complement(1362..1814)
                     /locus_tag="VFV99_27620"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5063188.1"
                     /translation="MGEKRRNTRTFEVERTTGSHEILDRLFVYGTLRQGQTARSLVAN
                     QITKCEKASTTGSIYAFPMGHPGYVEGDGKGRVEGEVIWLTELAATFGLLDAYEGQDF
                     VRVIRQVHLEDSNENVWAWVYTLSDPSAVKFGTLIQHGDWVRYWTEQG"
     gene            complement(1857..2864)
                     /locus_tag="VFV99_27625"
     CDS             complement(1857..2864)
                     /locus_tag="VFV99_27625"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5063189.1"
                     /translation="MSQVLQHVIEAIGPASQANAEAVRARIAQANAPVLEQLAARLAG
                     AQHTPKPRSAKRMIVVVAGDHGAGDPGIAMGADHPTVVAARAIADGSAALCQLARSSS
                     AVNGGAMPGSSIPPGGTPIVLVDAGSREPSHMPDIAIQLGRGPSLDILREPAMTVVDA
                     ALGLDAGIALSITLSEQSPDVLALGAIGVGSDVASAAILAAVTGQPPGALRDDVAEAA
                     GIRGMALRGAGALEILATFGGSDTAVLAGLILGAASMNVPVILDSYATGAAALIAAQL
                     ARPVTGYMIAAHRGSFTQPAILDHLGLSPAFDVGLGHGEGAGAAMVLPLLDQVTALAQ
                     R"
     gene            complement(2861..3919)
                     /locus_tag="VFV99_27630"
     CDS             complement(2861..3919)
                     /locus_tag="VFV99_27630"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5063190.1"
                     /translation="MSGFVSGRGARLTPKRLAVVLGTCAIVCALVCVLAPLLGVIMTP
                     GGRRLQLLGLDAFDVGSAEHRLFMVRLPRVLGAMLVGAALSAAGCAMQALLRNPLAEP
                     YTLGISSGSSLAAVLAIRLGVEGAFGGAGVGVSALLGAMATLVLIERLGRMGRHLPPA
                     TIVLAGVTISMFCSAASMLIQYTSDFSDVSHMLAWMIGALDSVRLGSVEYAAVPIGVG
                     LVALILYGRELNALAAGPDVAAALGVAVARTEVTIFGLASLLVGVAIAIAGPIGFVGL
                     IVPHTLRALLGPDHRLLVPASVFGGATLLVVCDTLARTLTAPAHLPTGAVTAVLGGPF
                     FIAILVQRKRYAALWGRV"
     gene            complement(4009..4773)
                     /locus_tag="VFV99_27635"
     CDS             complement(4009..4773)
                     /locus_tag="VFV99_27635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5063191.1"
                     /translation="MWLQLAQVDVALGGRTVVHGVSASVAQGEMVVIVGPNGAGKTTL
                     LRALAGFVAPSAGTVRVMGHDPARVARRVIAREMAYLPQQYELAFPFLVEEIVLLGRY
                     ANQRGLGLPSEADMAAAHAAMKTCDVEALATRRFDELSGGEARRVILAQAVCQGAKTL
                     LLDEPTAGLDPAHARAVFEMLSGHCNAGGAAVVVTHDINLALRHGGTMWLVANGMLAA
                     NGAPAEVLASAAAHKAFELAIHVGELPSGARFAVPS"
     gene            4828..5922
                     /locus_tag="VFV99_27640"
     CDS             4828..5922
                     /locus_tag="VFV99_27640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5063192.1"
                     /translation="MRRRNDRVVMNLPVEVITSGKKVRAVSQDLSPMGMFIRLSPPLP
                     PGTVIQIVISPNGHRQVITGQVTHALNDIEARTLGRFPGNGVMFREAMRPSEQAFLDA
                     VSRLLESHAAARPHADLRILVADPQTRILERLSTALGSAGFLVATATNGIEAIGACLS
                     KTPDVALIERDMPVVDGLHVLQEMGRHGPLASVPVMMMSANATDLARLQAFQLGAADF
                     IPKPFTVLEVILRARRWARVSQHDTERVVLRGTLAEINLPTLLQMFEQEKKTGQLSVT
                     SDQLVAWIDFASGQIVRARSSEVHGSARDIVLKVLDWKHGFFELTAGAPTPGTVEIDA
                     TVTHLLLEHARLHDEAAREGSHGNGRADES"
     gene            complement(5977..6453)
                     /locus_tag="VFV99_27645"
     CDS             complement(5977..6453)
                     /locus_tag="VFV99_27645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEX5063193.1"
                     /translation="MEFTFFFTEASPFSQWHRCRFAVDGVTFNCAEQFMMHGKAVLFG
                     DAAIAAEILAADHPRTHKALGRKVKDFDGAVWERERMRIIKDGNRAKFTQNPELLAKL
                     LATKGTLMVEASPFDRIWGIGLGANDPRAKDPKQWKGKNLLGYVLTELRDELLATS"
     gene            complement(6539..7363)
                     /locus_tag="VFV99_27650"
     CDS             complement(6539..7363)
                     /locus_tag="VFV99_27650"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX5063194.1"
                     /translation="MRCALIILAVLLGCGSKREPAPSAQSRIVSLMPSATEIVAALGA
                     TKELVGVDEYSDYPDEVKALPKVGSFLAPNLEVIVQLTPTLVIVDDIHGQVAGSLHDH
                     GLATADCKMHALPDVKDCLRTVGAKLGRAKQADAVVEEIEHELDAAAAARPAKHPRVL
                     VIIDRESGGLGNLVAAGPGSWVDELLAVVGGNNVLAASGVRYPKISLEEVISGDPEVI
                     IDLSYDKNEARKAAAWKQVDVTAVRDNRVRLMDHAFLRGPSPRVKQALAALAEAIK"
     gene            7399..>8972
                     /locus_tag="VFV99_27655"
     CDS             7399..>8972
                     /locus_tag="VFV99_27655"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063195.1"
                     /translation="MPETDLKGTVLDGRYQVIEPIAEGAMGSVWRGERVKLGRAVAIK
                     IMHQELPDELASRQRFEREAKLMALLEHPNCVSVIDFGVHEDKPFLVMDLVRGTSLIE
                     ILDKQGHLDVAKSSDIIRQVLSGLAHAHERGIVHRDIKPANIMITEKAGLGQQVRILD
                     FGLARMQEATGTKLTTGIVVGTPNYMAPEQCKGGDIDARTDLYACGVVLFEMLTGRKP
                     FIADDPIQVVRKHLLEKAPTMASLTNTDFGELEAVVAKALAKSPNDRFASAVDMAKAV
                     ELASSNATKTSAAALYSRAVPTGGPAIGNQQATMSGWNVPQDGQSAAPPQSYPPPPEG
                     YPPPPQGYPPPPQGYPPPPQGYPPPAQGYPPPPQGYPPPPQGYPPPPVAAPPSVAAAG
                     AAQPVAPLPSVGAAGAAGAAGAAPSPIASSAPGAAVVAPPPVGAPRMPTPAAGARAVP
                     PPRGATPAAGARAVAPPPRGATPAAGSPAAPPAGATPAAGSPAGATPAAGARAVPPPR
                     VPTPAAGAPVAPGDVH"
BASE COUNT         1508 a         3114 c         2979 g         1371 t
ORIGIN      
        1 gtcgcgctcg tcgagcccgc cgacggcaag gcgctggtcg agctcgaaca gcggctcggc
       61 gcgaagatca cgccgtacgt ggtcccggag ctacgcgcgc tctactacct cgagaagcac
      121 ttcggcacgc cgcgccgcgc gcggttcagt cgcagcgcgc gtcccggcac cgatccgggc
      181 gagctgatcg gtgacgatcg ccgcaagaca cagcccgcgg gtggcatcgc gatgccgccg
      241 aagctcacgc tcgagccgcg ccggcgtcgc ccctcgcaag cgccgatgac gcagaccgtg
      301 ccgaccacgc tgtcgtacgg tgcggcgtgc gagcgcatcg acaccgcgtc gaaccgcgag
      361 caaattgccg acacgttcat cgactacgcg cgcggccggt gcgacgtgct cgtcgtcttc
      421 ctcgttcgcg atggcaatgc gctcggctgg cgtggctacg tggcgccgcc gaaccaggcg
      481 acgaagccga tcgaggagat ctcgctcccg ctgggtggcg cgtccgcgtt gcagtcggcg
      541 cacgacgctg gacagacgta cgtcggacag ccgccatcgc cggccaagcc gatcgaaggc
      601 acgctgtgga ccgcgctggg caccgagccg aagccgatcg aggtgctcgt cgtgccgatc
      661 ctcgtgaagc agcgcgccgt gaatttgatc tacacgcaca cgctcggtgg cacgccgccg
      721 cagtcgctga tcaccgagct gcaggacctc gcggcccgcg cgcagacttc gtacatgcga
      781 ctgattcgac agactcgcgg gtcctgacag acgtgcgcga attgtgatcg cgcagtgcgt
      841 gatcgctggc gcgatcggct ctgccggacg tgatctcacc gcacactgag gcccgatcgc
      901 tctgggacaa tgtacgtgct cccgcgtatg tccacgcatc ggaggggtct gttagtcaag
      961 gggtaagggt gcttcaaaac aggggtcaat caacggagat tcttcaacaa gttaccccaa
     1021 actgatcccc tgctcaggaa cttgattttg gctgccaaag agggaaagtc ctctcgtggc
     1081 gcaacgtttg tccctagcaa gtaacaaggt cgggcccggt acgatcgcag cagctctcgc
     1141 cgccctctca atggcggttc tcgctcactt cttcatctga ttagccagct tcgggggaag
     1201 cctgctgaca tcggattgag gagtctggtg cgtcttgcga cgtgccgggg ttcgttttcg
     1261 cggccgtcat gtgacgtacc ggggttcgct ttcgctgcct cgcgacgtac cggggttagt
     1321 tttcgcggcc agattggccg gccggcagtt agctcctgcc actaaccctg ttcggtccaa
     1381 taccgaaccc agtcgccgtg ctggatcaag gtcccgaact tcacggccga gggatcggac
     1441 agcgtgtaga cccacgccca gacgttctcg ttgctgtcct cgaggtggac ctggcgaatt
     1501 acccggacga agtcttggcc ttcgtaggcg tcgaggaggc cgaacgtcgc ggcgagctcg
     1561 gtcagccaga tcacctcgcc ctcgacccga cctttgccgt cgccctcgac gtagccaggg
     1621 tggcccatcg ggaacgcgta gatcgatccc gtcgtcgacg ctttctcgca cttcgtgatc
     1681 tggttggcga cgagcgagcg cgcggtctgg ccctggcgca gcgtcccgta cacgaacaag
     1741 cgatcgagga tctcgtgtga gcccgtcgtg cgttcgactt cgaacgtccg cgtgtttctc
     1801 ctcttctcgc ccatgccgtt tgtctaacgc cgagtcgcga gcggtgccag cgtatcctaa
     1861 cgctgtgcga gtgccgtcac ctgatcgagt agcgggagca ccatcgcagc tcccgcgccc
     1921 tcgccgtgac cgaggccgac gtcgaacgcc ggtgacaggc cgaggtgatc gagaatcgct
     1981 ggttgcgtga agctcccgcg atgagccgcg atcatgtagc cggtaaccgg tcgcgcgagc
     2041 tgcgcagcga tcagggccgc ggcgccggtc gcgtagctat cgagaatcac gggcacgttc
     2101 atcgacgctg cgccgaggat cagtccggcg agcacggcgg tgtccgagcc accaaacgtc
     2161 gccaggatct ccagcgcgcc cgcgccacgc agcgccatgc cacgaatgcc ggcagcttct
     2221 gcgacgtcgt cgcgcaatgc gccgggcggc tggcccgtca ctgcagcgag gatcgcggcg
     2281 gatgcgacgt cgctgccgac cccgatcgcg ccgagcgcga gcacgtccgg tgattgctcg
     2341 gacagcgtga tcgacagcgc gatgccggca tcgaggccca gcgccgcgtc gacgaccgtc
     2401 atcgcgggct cgcgcaggat gtcgaggctc ggtccgcgtc cgagctgaat ggcaatgtcc
     2461 ggcatgtggc tcggctcgcg cgagcccgca tcgaccagca cgatcggcgt accgccaggc
     2521 ggaatgctgg aacccggcat cgcgccgccg ttcaccgcgc tcgacgatcg cgcgagctgg
     2581 cagagcgccg cgctgccatc ggcgatcgca cgcgccgcaa ccacggtcgg atgatcggcg
     2641 cccatcgcga tgcctggatc gcccgcgcca tgatccccgg cgaccaccac gatcatccgc
     2701 ttcgcagaac gcggcttcgg cgtgtgctgc gcacctgcga gccgagctgc gagctgctcg
     2761 agcaccggcg cgttcgcctg cgcgatacgc gcgcgcacgg cctcggcgtt cgcctgcgaa
     2821 gccggcccga tcgcctcgat cacgtgctgc agcacctggc tcatacgcgt ccccagagcg
     2881 cagcgtagcg cttgcgctgg acgaggatcg caatgaagaa cggcccaccg agcaccgcgg
     2941 tgaccgcacc ggtcggcaag tgtgcgggcg cggtcagcgt gcgcgcgagc gtgtcgcaga
     3001 cgacgagcag cgtcgcgccg ccgaacaccg acgccggtac gagcaaccgg tgatcggggc
     3061 cgagcagcgc gcgcagcgtg tgcggcacga tgaggccgac gaaaccgatc ggtcctgcaa
     3121 tcgcgatcgc gacgccgacc aagagtgatg cgagcccgaa gatcgtcact tcggtgcgcg
     3181 cgacggcaac gccgagcgct gccgcgacgt cgggtccagc cgccagagcg ttgagctcgc
     3241 ggccgtacag gatgagcgcg acgagcccga cgccgatcgg cacagccgcg tactcgaccg
     3301 acccgagccg cacgctgtcg agcgcgccga tcatccacgc gagcatgtgg ctgacgtcgg
     3361 agaaatcgct cgtgtactgg atcagcatcg acgccgccga gcagaacatc gagatcgtga
     3421 cgccggcgag cacgatcgtc gcgggcggca aatgacggcc catgcggccg agtcgctcga
     3481 tcaatacgag cgtcgccatc gcgccgagca gcgcactgac gccaaccccg gcaccgccga
     3541 atgcgccctc gaccccgagc cggatcgcga gcaccgcggc gagcgagctg cccgacgaga
     3601 tgccgagcgt gtacggctcg gcgagtgggt tgcgcagcag cgcctgcatc gcgcagcccg
     3661 cggccgacag cgcggcaccg acgagcatcg cgccgagcac gcggggtagc cgcaccatga
     3721 acaaccggtg ctccgccgac ccgacgtcga acgcatcgag cccgagcagc tgcaggcgac
     3781 ggccgcccgg cgtcatgatc acgccgagca gcggcgcgag cacgcagacc agcgcgcaca
     3841 cgatggcgca ggtcccgagc accacggcga gccgcttggg cgtgaggcgc gcgccgcgtc
     3901 cgctgacgaa gccgctcatg acggcaccgc acttcgcacg ccgccgctca tgacgacacc
     3961 gcaattcgcc cgccgctcat gaccacaccg caattcgcgc gccgccgctc atgacggcac
     4021 cgcaaatcgc gcgccgctcg gcaattcacc gacgtggatc gcgagctcga acgccttgtg
     4081 cgccgccgcc gatgcgagga cctctgccgg cgcaccgttg gcggcgagca tgccattggc
     4141 gacgagccac atcgtgccgc cgtgacgaag cgcgagattg atgtcgtggg tgacgacgac
     4201 cgccgcgccg cccgcgttgc aatggccgct cagcatctcg aatacggcgc gggcgtgtgc
     4261 cggatcgagt ccggcggtcg gctcgtcgag cagcagcgtc ttcgcgccct ggcagaccgc
     4321 ctgcgcgagg atcacccggc gtgcttcgcc gcccgatagc tcgtcgaagc gccgcgtcgc
     4381 cagtgcctcg acgtcgcacg tcttcatcgc cgcgtgggcc gcggccatgt ctgcctccga
     4441 cggcaacccg agcccgcgtt ggttcgcata gcggccgagc agcacgattt cctcgacgag
     4501 aaacggaaac gcgagctcgt actgctgggg cagatacgcc atctcgcgcg cgatcacccg
     4561 acgggcaacc cgcgctggat cgtggcccat cacccgcacc gtacctgcgc tcggcgcgac
     4621 aaacccggcg agcgcacgga gcagcgtcgt cttgccagcg ccgtttggcc cgacgatgac
     4681 caccatctcg ccctgcgcaa cgctcgcact cacaccatgc acgacagtgc gaccgccgag
     4741 cgcgacatct acctgggcga gttggagcca cacggagcgc ttttaccaag tagcaacgtc
     4801 tgcggaaacg cggtagccaa gagactcgtg cgtcgccgta acgatcgcgt cgtcatgaac
     4861 ctgccggtcg aggtgatcac cagcgggaag aaagtgcgcg cggtctcgca ggacctgtcg
     4921 ccgatgggca tgtttatccg cctgtcgccg ccgctgcccc caggcacggt gatccagatc
     4981 gtcatttcac cgaacgggca ccgtcaggtg attaccggcc aggtcacgca tgcgctcaac
     5041 gatatcgagg cgcgcacgct gggtcggttc ccaggcaacg gcgtcatgtt ccgcgaggcg
     5101 atgcgaccga gcgaacaagc gttcctcgat gcggtcagcc ggctgctcga gagccacgcg
     5161 gcggcgcggc cgcacgccga cctacgcatc ctcgtcgccg atccacagac ccgcatcctc
     5221 gagcggttgt cgaccgcgct cggtagcgcc ggctttctcg tcgcgaccgc gaccaacggc
     5281 atcgaagcga tcggcgcgtg tctgtcgaag acgcccgacg tcgcattgat cgagcgcgac
     5341 atgccggtcg tcgacggact tcacgtgctc caggagatgg gccgccacgg tccgctggcg
     5401 agcgtgccgg tgatgatgat gtcggcgaac gcgaccgacc tcgcgcgcct gcaggcgttc
     5461 cagctcggtg ccgccgattt cattcccaaa ccgttcacgg tcctcgaggt gatcctgcgt
     5521 gcgcgacgct gggcacgcgt gagtcagcac gataccgagc gcgtcgtgct gcgcggcacg
     5581 ctcgccgaga tcaacttgcc gacgctgctc cagatgttcg agcaggagaa gaagaccggc
     5641 cagctgtcgg tcacgagcga ccagctcgtc gcgtggatcg atttcgcgag cggtcagatc
     5701 gtgcgagcgc gctcgtcgga ggttcacggc tccgcgcgcg acatcgtgct caaggtgctc
     5761 gactggaagc acggcttctt cgagctcacc gcgggcgcgc cgacgccggg cacggtcgag
     5821 atcgacgcca cggtcacgca tctgttgctc gagcacgcgc ggctacacga cgaagcagct
     5881 cgcgagggct cgcacggcaa cggacgggcg gacgagagct agctcgcggc gagtagctcg
     5941 cgagggctcg cacggcaacg gacgcgcgga cgagagctag ctcgttgcga gtagctcgtc
     6001 tcgcagctcg gtcagcacgt agccgagcag gttcttgccc ttccactgct tcggatcctt
     6061 cgcgcgcgga tcgttggcgc caaggccgat gccccagatc cgatcgaacg ggctcgcttc
     6121 gaccatgagc gtgcccttcg tcgccagcag tttcgcgagc agctccgggt tctgggtgaa
     6181 cttggcgcga ttgccgtcct tgatgatgcg catgcgttcg cgctcccaca cggcgccgtc
     6241 gaagtccttc accttgcgac cgagcgcctt gtgcgtgcgc ggatgatcgg ccgcgaggat
     6301 ctcggctgcg atcgcggcat cgccgaacag cacggccttg ccatgcatca tgaactgctc
     6361 ggcgcagttg aacgtcacgc catccactgc gaagcgacag cggtgccact gcgagaacgg
     6421 cgacgcctcg gtgaagaaga acgtgaattc catggtgtaa agactacacg aacacgctgg
     6481 cgcggcaaca ccgacggaga tcccggtccg gagcgccgca gaatcgttgc tcgatacgct
     6541 acttgatggc ctcggccagg gcggccagcg cctgcttcac ccgcggcgat ggtccgcgca
     6601 agaacgcgtg gtccatcagg cgcacgcggt tgtcgcgcac ggccgtcacg tcgacttgct
     6661 tccacgccgc ggccttgcgt gcctcgttct tgtcgtatga cagatcgatg atcacctcgg
     6721 ggtcaccgct gatcacttcc tccagcgaga tcttcggata gcgcacgcct gacgcagcga
     6781 gcacgttgtt cccgccgacg accgcgagca gctcgtcgac ccagctgccc gggccagccg
     6841 cgacgagatt gccgagcccg cctgattcac gatcgatgat gacgagcacg cgcggatgtt
     6901 ttgcggggcg cgcggccgct gcggcatcga gctcgtgctc gatctcctcg acgacggcgt
     6961 ccgcttgttt cgcgcgtccg agcttggcac cgacggtgcg caggcagtcc ttgacgtcgg
     7021 gtagcgcgtg catcttgcag tcggccgtgg cgagcccgtg gtcgtgcagc gagcccgcga
     7081 cctggccgtg gatgtcgtcg acgatgacca gcgtcggcgt gagctggacg atgacctcga
     7141 ggttcggcgc gaggaacgag ccgaccttgg gtaacgcctt cacttcatcg ggatagtccg
     7201 agtactcgtc gaccccgacg agctccttgg tcgcgccgag cgctgcgacg atctcggtcg
     7261 ccgatggcat gagtgacacg atgcgcgact gcgccgatgg tgccggttcg cgtttgctcc
     7321 cacagcccag gagaacggca aggatgatca gggcgcagcg catatgctgt cggtagcaca
     7381 ggctggtatc gtcgtgccgt gcctgagacc gacctcaaag ggaccgtgct cgacggtcgc
     7441 taccaggtca tcgagccgat cgccgaaggc gcgatgggca gcgtttggcg cggcgagcgg
     7501 gtgaagctcg gccgcgcggt cgcgatcaag atcatgcacc aggagctccc agacgagctc
     7561 gcgagccgcc agcgcttcga gcgcgaagcc aagctcatgg cgctgctcga gcacccgaat
     7621 tgcgtgtcgg tgatcgattt cggcgtccac gaggacaagc cgtttctcgt gatggacctc
     7681 gtgcgcggca cgagcctgat cgagatcctc gacaagcagg gccacctcga cgtcgcgaag
     7741 agcagcgaca tcattcgcca ggtgctgtcc gggctcgcgc acgcgcacga gcgcggcatc
     7801 gttcaccgcg acatcaagcc tgcgaacatc atgatcacgg agaaggcggg gctcggccag
     7861 caggtccgca ttttggactt cggcctcgcg cggatgcagg aagcgacggg cacgaagctg
     7921 acgacgggca tcgtcgtcgg tacgccgaac tacatggcgc ccgagcaatg caaaggcggc
     7981 gatatcgacg cgcgcaccga tctctacgcg tgcggcgtgg tgctgttcga gatgctcacc
     8041 gggcggaagc cgttcatcgc ggacgatccg atccaggtcg ttcgcaagca cctgctcgag
     8101 aaggcgccga cgatggcgtc gctcaccaac accgatttcg gcgagctcga ggccgtggtc
     8161 gccaaggcgc tcgcaaagtc gccgaatgat cggttcgcgt cggccgtcga catggcgaag
     8221 gccgtcgaat tggcgtcgag caacgcgacg aagacgagcg ccgcagcgct gtattcgcgc
     8281 gcggtgccga cgggcgggcc ggcgatcgga aatcagcaag cgacgatgtc gggctggaac
     8341 gtgccgcagg acgggcagtc cgcggcgcca ccgcaaagct atccgccgcc accggaaggc
     8401 tacccgccac caccgcaagg ctatccgccg ccaccgcaag gctacccacc gccaccgcaa
     8461 ggctacccgc cgccagcgca aggctacccg cctccgccgc aaggttatcc gcctccgccg
     8521 caaggctatc cacctccgcc ggtcgctgcg ccgccatcgg tcgctgcggc cggtgcggca
     8581 cagcccgtcg cgccattgcc ttcggtcggt gcggccggtg cggccggtgc ggccggtgcg
     8641 gctccgtcgc ccatcgcatc atcggcgcca ggcgctgcag tcgttgcacc gccgcccgtc
     8701 ggtgcgccgc gcatgccgac gccagctgcc ggagcacgag ccgttccgcc gccgcgcgga
     8761 gcgacgccgg ctgccggagc acgagcggtc gctccgccgc cacgcggggc gacgccggcc
     8821 gccggttcgc cagctgcgcc gccagcagga gcgacgccgg ccgcgggttc gccagcagga
     8881 gcgacgccgg ccgcaggagc acgagccgtt ccgccgccgc gcgtgccgac tccagccgcc
     8941 ggagcgccgg tcgcgccggg cgacgtgcac ag
//
LOCUS       DASUFX010000685         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_387449,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000685 DASUFX010000000
VERSION     DASUFX010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387449"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFV99_27660"
     CDS             <1..436
                     /locus_tag="VFV99_27660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063196.1"
                     /translation="GAAAINVPASGWLLICTALLATFLGLGKRAHELAWAERTGQAHE
                     TRASLAGYNIPVVRGAMFVIGLVTCIAYLGYTVDKHTIAYFHTDELPYSSPFVLLGIL
                     RFLSLALWNPSEESPTEAMLKDPWFLLNLVAAAATVLYIIYR"
     gene            complement(451..2217)
                     /locus_tag="VFV99_27665"
     CDS             complement(451..2217)
                     /locus_tag="VFV99_27665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5063197.1"
                     /translation="MIGGRRRRLRPEVERQLEQIAFDRLRIFVPIVIGGILLALFMSA
                     PIGIPLPASVLFINGAMLTFAITLYVALRRRMIPSRFVHVVAAMVWLVTPINTLSSYV
                     ITDNATLVLPLMIEMATAALMVDTVWTLLVTVPVVAIGVPLSLVAGDAPIYPTGVVGL
                     WVIALIMQIWVRRWTIAAETHRLDVENTAKQLALELDERKRAQAESEKLRDQFVHAQR
                     LDAVGTLAAGLAHDMNNILGGILAFAEVLYAEAKDKHVRADLARIRQEAERGAALTRS
                     LLAFSRRGAYRRRPILLHSVLDDMMPLLERTLGKSITVTRTDGPPTIIDGDPAQLGQV
                     LLNLCLNAKDALSETVTITTDEVEVSAGQVDGLAPGRYAKLVIKDTGTGMDAETRKRA
                     FEPFFTTKPVGKGTGLGLAMVFGAVQAHGGSITLDSAVGSGTTITIYIPASEASPALP
                     ASDAKSRPRNRGVVLVVDDEPVVRTATARLVEQLGLTAVTAADGEQAVEVYKQRQKEV
                     VLVLLDMMMPKMDGPTTYRALRKLGRTPVLLVSGYAADLAAQDLLDTGADGFLEKPYT
                     AEQLGAEIDRILGRAEPAQQQA"
     gene            complement(2214..3527)
                     /locus_tag="VFV99_27670"
     CDS             complement(2214..3527)
                     /locus_tag="VFV99_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5063198.1"
                     /translation="MDLFAQSARKRQVGQPLAERMRPRSLDELVGQAHLVGPGRILSN
                     LAPGRAIPSLILWGPPGSGKTTLARLLGETLKAELTSLSAVEAGVKEVREAIAKAGER
                     RDQFGARTLLFIDEIHRFSKTQQDALLPHVEAGTVTLIGATTENPSFGVVAALLSRCR
                     IVRLEALADDELAGLVTRAIADRERGLGPMNLTIDPEVTAQVVAAAAGDARRLYSVLE
                     VAASLAGDNGAITAQHVAEAAQGKSLLYDKAGDEHYGVVSAFIKSMRGSDPDAAVYWM
                     TRMLEAGEDPRFVFRRMVIFAAEDVGNADPQALVVATAALQAFELVGLPEGVLPLTQA
                     VTYLALAPKSNRALTTYANARKLVKEHGALPVPKQLRPGSNAAGRAQGHGEGYKYPHD
                     FEGAYVPEDYLPDELVGVTIYEPGDSGFECELAARLEALREKRRK"
     gene            3667..>3941
                     /locus_tag="VFV99_27675"
     CDS             3667..>3941
                     /locus_tag="VFV99_27675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063199.1"
                     /translation="MGPAICLVMLLETVFQYRTLLGKCDLGCGLDWDEIDAVQRIENA
                     FAADRKDGRKFRRQVVELVGIMRGDMINDRVDVIEIGPGGMVCRDAP"
BASE COUNT          632 a         1311 c         1307 g          691 t
ORIGIN      
        1 cggcgccgcg gcgatcaacg tgccggcgtc aggctggttg ctcatctgca ccgcgctgct
       61 cgcgacattc ctcggcctcg gcaagcgcgc acacgagcta gcgtgggccg agcgcacggg
      121 ccaggcgcac gagacccgcg cgtcgctcgc cggctacaac attccggtcg tccgcggcgc
      181 gatgttcgtg atcggcctcg tcacctgcat cgcgtacctc ggctacaccg tcgacaagca
      241 cacgatcgcg tacttccata ccgacgagct gccctactcc tcgccgttcg tgttgctcgg
      301 catcctgcgc ttcctgtcgc tcgcgctctg gaacccgagc gaggagtcgc cgaccgaagc
      361 gatgctgaag gatccgtggt tccttctgaa cctggtcgct gccgccgcga ccgtcctcta
      421 catcatttac cgctgattgc ggctacggct tcacgcctgc tgctgcgccg gctcggcgcg
      481 gccgaggatg cgatcgatct cggcgcctag ctgttcggcc gtgtacggct tctcgaggaa
      541 gccgtcggcg ccggtgtcca gcaggtcctg cgcggcgaga tcggcggcgt agcccgacac
      601 gagcagtacc ggcgtgcggc cgagcttgcg cagcgcgcgg taggtggtcg gcccgtccat
      661 cttcggcatc atcatgtcga gcagcacgag cacgacttcc ttctggcgct gcttgtagac
      721 ctcgaccgcc tgctcgccgt cggcggcggt caccgcggtg agaccgagct gctcgacgag
      781 gcgcgcggtc gcggtgcgca cgacgggctc gtcatcgacg acgagcacga caccgcggtt
      841 gcgcggccgc gacttggcat cggatgccgg cagcgccggg ctcgcctcgc tcgccgggat
      901 gtagatcgtg atcgtcgtgc cgctgccgac cgcgctgtcg agcgtgatcg agccaccgtg
      961 cgcctggacg gcgccgaaca ccatcgcgag gccgagcccc gtgcccttgc cgaccggttt
     1021 cgtcgtgaag aacggctcga acgcgcgctt cctggtctcg gcatccatgc cggtgccggt
     1081 atccttgatc acgagcttcg catagcggcc gggcgccagg ccgtcgactt gcccggcgga
     1141 gacttcgacc tcgtcggtcg tgatcgtgac cgtctcgctc agcgcgtcct tcgcgttgag
     1201 gcacaggttg agcagcacct gcccgagctg cgccggatcg ccatcgatga tcgtcggcgg
     1261 accatccgta cgcgtgaccg tgatgctctt gccgagcgtg cgctcgagca gaggcatcat
     1321 gtcatcgagc accgagtgca gcaggatcgg gcggcggcgg tacgcaccac ggcgcgagaa
     1381 cgcgagcagg ctgcgcgtga gtgcggcgcc gcgctcggct tcctggcgaa tgcgtgcgag
     1441 gtcggcgcgc acgtgtttgt ccttggcctc ggcgtagagg acctcggcga acgcgagaat
     1501 gccgccgagg atgttgttca tgtcgtgtgc gaggccagcg gccagcgtgc cgacggcgtc
     1561 caggcgttgc gcgtggacga actgatcgcg gagcttctcg ctctcggctt gcgcgcgttt
     1621 gcgttcgtcg agctcgagcg cgagctgctt cgcggtgttc tcgacatcga gccggtgggt
     1681 ctcggcggcg atcgtccagc ggcgcaccca gatctgcatg atgagcgcga tcacccagag
     1741 gccgacgaca ccggtcggat agatcggcgc gtcgcccgcg acgagcgaga gcggtacgcc
     1801 gatcgcgacg accgggacgg tgaccagcag cgtccagacg gtgtcgacca tcagcgcggc
     1861 ggtcgccatc tcgatcatga gcggcaacac gagcgtcgcg ttgtcggtga tgacgtagga
     1921 cgacaacgtg ttgatcggcg tgaccagcca gaccatcgcc gcgacgacgt ggacgaaccg
     1981 gctcgggatc atccggcggc gcagcgcgac gtacagcgtg atcgcgaacg tcaacatcgc
     2041 gccgttgatg aagagcaccg atgcgggcag cgggatgcca atcggcgcgc tcatgaacag
     2101 cgcgagcaag atgccgccga tgacgatcgg cacgaagatg cggaggcggt cgaacgcgat
     2161 ctgttcgagc tggcgttcta cttcaggtcg taaacgccgc cgccgccccc cgatcatttg
     2221 cgccgctttt cgcgaagtgc ttcgagtctg gcggcaagct cgcactcgaa cccggagtca
     2281 cccggctcgt agatcgtcac gcccaccaac tcgtccggca gataatcctc cggcacgtac
     2341 gccccttcga agtcgtgagg atatttgtaa ccctcgccgt gcccttgcgc gcgtccggcg
     2401 gcgttggaac cgggccgcaa ttgtttcggg accggcaacg caccgtgctc tttgacgagc
     2461 ttccgcgcgt tggcgtaggt cgtgagcgcg cggttcgact tcggcgcgag cgcgagatag
     2521 gtcaccgctt gggtcagcgg cagtacacct tcgggtaacc cgacgagctc gaacgcctgt
     2581 agcgcggcgg tcgcgacgac gagcgcctgc gggtccgcgt tgccgacatc ttcggcggca
     2641 aagatcacca tcctgcgaaa cacgaagcgc gggtcttcgc cggcctccag catgcgcgtc
     2701 atccaataga ccgcagcgtc tggatccgag ccccgcatgc tcttgatgaa tgcagagacg
     2761 acgccatagt gctcgtcgcc cgccttgtcg tagagcaggc tcttaccttg ggcggcttcc
     2821 gcgacatgtt gcgcagtgat cgcgccgttg tcacccgcga gcgacgcagc aacttcgagc
     2881 accgagtaga ggcgccgcgc atcaccagcg gcggcggcca ccacttgcgc tgtgacctcg
     2941 ggatcgatcg tgaggttcat cgggccgagt ccgcgctcgc ggtcggcgat cgcgcgcgtg
     3001 acgagacctg ccagctcgtc atcggcaagc gcttcgagcc gcacgatgcg gcagcgcgac
     3061 aacagcgcgg ccacgacgcc gaagctcggg ttctccgtgg tcgcgccgat cagcgtgacg
     3121 gtgccggctt cgacatgcgg gagcagcgcg tcctgctgcg tcttcgaaaa ccgatggatc
     3181 tcatcaatga acagcagcgt gcgcgcgccg aattgatcgc ggcgctcacc ggctttggcg
     3241 atcgcttcgc ggacttcctt gacgccagct tcgacggccg aaagcgaggt tagctctgcc
     3301 ttcaacgtct cgccgagcag gcgggcgagc gtggtcttgc cgctgccggg cggtccccac
     3361 aggatcagtg acggaatggc gcgccccggg gcgaggttgg acagaatgcg gcccggaccg
     3421 accagatgcg cttgcccgac gagctcgtcg agcgagcgcg gtcgcatccg ctcggccaat
     3481 ggctggccga cctgacgctt acgtgcgctt tgcgcgaaaa ggtccatagt actggccgat
     3541 ttatacacac atgtggccct gcgaccacgc gcccacaacc tggggacact acacccggca
     3601 aatcgaccgt ccggctaggc gcgacgatcc acgatttcga ttccttaacc ctaccgtacc
     3661 cgtggcatgg gccctgctat ttgcctggtc atgctgctcg agaccgtctt tcagtatcgc
     3721 accctgctag ggaagtgcga tctgggctgc ggtctcgatt gggacgagat cgatgccgtc
     3781 caacgcatcg agaatgcgtt cgcagccgac cgcaaggacg gccgtaagtt ccgtcgtcag
     3841 gtcgtcgagc tcgtcggcat catgcgcggc gacatgatca atgaccgcgt cgatgtgatc
     3901 gagatcgggc caggcggcat ggtgtgtcgt gacgcaccct t
//
LOCUS       DASUFX010000686         7350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_387451,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000686 DASUFX010000000
VERSION     DASUFX010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387451"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1561)
                     /locus_tag="VFV99_27680"
     CDS             complement(266..1561)
                     /locus_tag="VFV99_27680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063200.1"
                     /translation="MLSRLVPIALVAWFAVGCEKPTHENIEKWAATQKGPAKLKAAFL
                     NDGLDADLSAHAAAVMVKTGRDNEVKAELDHMNAQRRTAVIGKLAPRLWEIARIEGEM
                     LRPAPQQTQAKDALVVIRKYADPAVRTQIDGYLIDWYAVPSYEGRANSGAFLGAMVMR
                     TIGAPAAKKMMAVVNGIIASPGQETSKKRIGDELMLAVAVTGDPEAVKYILDIARMDR
                     GDKSLSTRAMGALYRAYVNPEGLFDVVEPGPALTPNLDELAKVAQDDRMPGAAANSAV
                     ALIRSVGPPQCLPPLLAMIPHPHADPMFRYMGPDHALKCGGAKAIKDVVRAMPNQPYE
                     KESLAGSVVLGISKLEPRDQVLASLRDLLGDQSLIARWVAVETLAAMKSVEDAPKIAS
                     VKGGGEKLVGFWGDGSGKPEPTLGQRAKELSTQLGKPSK"
     gene            1596..3065
                     /locus_tag="VFV99_27685"
     CDS             1596..3065
                     /locus_tag="VFV99_27685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063201.1"
                     /translation="MSRVTCLAVLCALHASAFASPGGDPTQGRAVFTGAATANPTSIE
                     INPAALGLGSRNEMYFAATGTLDHLSIDLSRFDINTGTFSPGEPVSGNLLSPGGMVAG
                     VWHSGVNGRVTLGVAVSTSPAEGWLEDKNALRYFTLGGSYRTLAPSIASSFKLSGRVH
                     LGIGLSVHTSFMRLKFARDAALAAGRDPVLGIGSDCGGSPCGIGNPDATQRYTVDVRS
                     DYVALDNVVGTLGVVVRLAKETWLGISYHTPPGLSVQTELSGTMDVVKAPRDGGGTVT
                     GGATVYLSQPASIDAELTSRLPANLDLHVGLRWEDLFRLQAYDARGYGSKFPSAGIPE
                     QQLRVRGFYDQSWPARFRTMGAWAGIEQTERDFPLNVGARIGFETASLPDDRTSPMTI
                     APMSATVDTGLQYRITRDRDVIPQVYLLVTYGLQYFPRVHVTDSAFDPRDAIACYDSN
                     HDYSTNACEAVRNGYAIDTGVGDYHRIQQAARIAVRLTW"
     gene            3070..4206
                     /locus_tag="VFV99_27690"
     CDS             3070..4206
                     /locus_tag="VFV99_27690"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5063202.1"
                     /translation="MNAPPIVVYGATGLIGGRVCAALDAVDVPFIAAGRRRAALEKVS
                     TLVGATDWRVAELEHDALVAAFNGAAVVVNCAGPLAEVAEPVLLATLAAGAHYVDLGG
                     DQLAIHALFERHDAAARKAGRVVVPGCGLNCAIGDWASAWAAMNVCGVHDEGDIVRHA
                     PATRLAEDKPLDEIAVSYIFDDLVLSPSSQKAVFGNLHSRGLVWHRDRWEHVAQAAER
                     RKVNAGPAMGGQRDAVSFPGGDVITVPRHVAARSVQTFVSTTRSTAATAALRLLARAM
                     PLVPKRATELLAPYQPHEEDYARTRFAVIAQARRGFAAAQVVVSGDDQYRASAGIAAW
                     VARTLVSRGAGPIGMRAPSELFRPEPALRQVGETIGLSIEPSFG"
     gene            4249..5553
                     /locus_tag="VFV99_27695"
     CDS             4249..5553
                     /locus_tag="VFV99_27695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5063203.1"
                     /translation="MSSWQLEAEAPTGRGVTVRRYRMGNGLGLIAAIDRRAPIVALQT
                     WYRVGSRHERPGATGMAHLFEHLMFGQTEKLPPGEFDRLVERTGGESNAATWVDWTYY
                     RLSLPARDLELGIKLESERMQHLVLEPTPVEAERDVVTNERRERVEDDVDGWLDEQLM
                     AHAFTEHPYRWPTIGWMEDIRALALPDIRAFYRTWYAPNNATIVCVGDFDDEKLRELI
                     AQHYGHIEPAQLPDVARIVEPEQTKERIVRAPKPIATDRLLVGYKAPGQDDPDWAALE
                     IVSTLLAGCPSARLHRKLVIEKEAASSVDAQLTPFRDPSLLRLAVTTARGHNADEILE
                     TIDHEIAALVETPPSAKEVEKAKALAETDFWSSLVDVDGKAEAFGHYETALGDFRKLT
                     VIAERLTQVTADDVARVVRTYLMPMKRTIVIAEPESDEGDEE"
     gene            5550..6839
                     /locus_tag="VFV99_27700"
     CDS             5550..6839
                     /locus_tag="VFV99_27700"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5063204.1"
                     /translation="MSSPVVIVEASPDTPLVWFDVAIRGGAAADPVGVEGLHRHAALL
                     ARRGAGSRDRHALDDTLDSLGAAVDISVSRDAVTLSGLSLSRHLDAVIDLCADILAEP
                     RLSEDEHARLLRETPQVLDEIRDDDSALATRWFDWVCSPGHPYGRTSLGTESSLEKIS
                     RPAATELWKREVVSNNMVIGLAGDIDDASATRIVQRLTERIPTGAKAPYKEDRSDETG
                     AKRGRRVILVNKSDRTQAQLRIGHLALRYGHPDTAALALAEAIFGGMFSSRLMQEIRV
                     KRGWSYGAGCALRRSRLPHWFEIWTAAAIDVAGPAVGLILELLADYAANGPTDDEVDF
                     ARSYLIGAMPFHVATARQRMQLAVRDAVFELPAGYTADLPKALGQLTADDVRAACKRH
                     LRPEDAVTVAVTTAENAQQALAAAGAGPMTIVDHDEY"
     gene            complement(6863..>7350)
                     /locus_tag="VFV99_27705"
     CDS             complement(6863..>7350)
                     /locus_tag="VFV99_27705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063205.1"
                     /translation="DEEITVEDIFALAAQHRTTFDTETRRSYYVIFVSGHFADQNGVQ
                     YGVLGISIGNTITMFKDVIKTTNIVLAPNVVRYVEQSTLVHELAHSIGLVDNGIPMVA
                     PHKDAPHGAHCNDQNCVMFWQNDGSQDAARFAQQKVLTGSSLLFDDQCLADVDAITGG
                     P"
BASE COUNT         1215 a         2458 c         2433 g         1244 t
ORIGIN      
        1 ggcggcgcgc cgggcgactg gtagttcttc gaccgcgcgt agtaccgcat cgcctcgttg
       61 acgaggtagt cgatagagca atcgaagtcg ttcgccattt gctcgaacgt ctcccaaagc
      121 acgtcgcggc aataaaagtg ccgcatcgtc ttcttgctct gatccattcg cgggttactc
      181 ctagacagtc cgcgccaacg ccagctccct ctcccgaggc gacttctacc ttactacatc
      241 gtcccgaaag acgcgaggaa gcgggctatt tggacggctt gccgagctgg gtcgacagct
      301 cttttgcgcg ctgccccaaa gtgggctccg gcttacccga accgtccccc caaaacccga
      361 cgagcttctc cccgcctccc ttcacggacg cgatcttggg cgcatcctcg acggatttca
      421 tggcggcgag ggtctcgacc gcgacccagc gggcgatcag gctctggtcg cccaggagat
      481 cgcgcaagct tgcgaggact tggtcgcggg gctcgagctt ggaaataccc aggaccacgg
      541 agccggcgag actctccttc tcataaggct ggttgggcat ggcgcggaca acgtccttga
      601 tcgcctttgc gccaccgcac ttgagcgcgt ggtccggccc catgtaccgg aacatcgggt
      661 ctgcatgtgg gtgtggaatc atcgccaaaa gcggcggcaa gcactgcggc ggaccgactg
      721 aacggatgag cgccactgcc gagttggcgg ccgcgccggg catgcggtcg tcctgggcga
      781 ccttggcgag ctcgtcgagg ttcggggtca gcgccggccc cggctcgacc acatcaaaca
      841 agccctccgg gttcacgtag gcacggtaga gcgcgcccat cgcacgggtc gatagcgact
      901 tgtcgccgcg atccatgcgc gcgatgtcga ggatgtattt gacggcttcc ggatcgcccg
      961 tgaccgcgac ggcgagcatg agctcgtcgc cgatgcgctt cttcgacgtc tcctggccgg
     1021 gcgacgcgat gatgccgttc acgacggcca tcatcttctt cgcggccggc gctccgatcg
     1081 tgcgcatgac catcgcaccg agaaacgcgc ccgagttcgc gcgtccctcg tacgacggca
     1141 ccgcgtacca atcgatcaga tagccgtcga tctgcgtacg cacggctggg tcggcgtact
     1201 tgcgaattac tacgagtgcg tccttcgcct gcgtctgttg cggcgccggc ctcagcatct
     1261 caccttcgat gcgtgcgatc tcccacagcc gcggcgcgag cttgccgatc accgcagtgc
     1321 gccgctgcgc attcatgtga tcgagctccg ccttcacctc gttgtcgcgg ccggttttca
     1381 ccatgaccgc ggcggcgtgc gccgacagat cagcgtcgag gccgtcgttg agaaaggctg
     1441 ccttcagctt ggccgggccc ttctgcgtgg cggcccattt ttcgatgttc tcgtgagtgg
     1501 gcttctcgca ccccaccgca aaccacgcga cgagagcgat cggtacaagg cgcgacaaca
     1561 tggcggcagc ttatcccggt gatacgtttc gcgcgatgtc tcgcgtgacc tgcctggcgg
     1621 tgctgtgcgc cctgcacgcg agtgcgttcg cgtcaccggg cggcgatccg acgcaaggtc
     1681 gcgccgtgtt caccggcgcg gcgaccgcga atccgacgtc gatcgagatc aatccggcgg
     1741 cgctcggcct cggtagtcgg aacgagatgt acttcgcggc caccggcaca ctcgatcacc
     1801 tctcgatcga tctgagcagg ttcgacatca acaccggaac gttctcgccg ggtgagccgg
     1861 tcagcggcaa tctgctgtcg cccggtggga tggtcgccgg cgtctggcac agcggcgtca
     1921 acggccgcgt cacgctcggc gtcgcggtgt cgacgtcgcc ggcagagggc tggctcgagg
     1981 acaagaacgc gctgcgctac ttcacgctcg gcggctcgta ccgcacgtta gcgccgtcga
     2041 tcgcgagcag cttcaagctg tcgggccgcg tgcacctcgg catcggcctg tcggtccaca
     2101 cctcgttcat gcgtctgaaa ttcgcgcgcg atgcggcgct cgccgctggc cgcgatccgg
     2161 tgctcggtat cggcagcgac tgcggcggct cgccgtgtgg catcgggaat cctgacgcaa
     2221 cccagcgcta taccgtcgat gtccgctcgg actacgtcgc gctcgacaac gtcgtcggca
     2281 cgctcggcgt cgtcgtccgg ctcgcgaaag agacctggct cggcatcagc tatcacacgc
     2341 cacccgggct gtcggtgcag accgagctca gcggcacgat ggacgtcgtc aaggcgccgc
     2401 gcgacggcgg aggtacggtc accggcggcg cgacggtcta cttgtcgcag cccgcgagca
     2461 tcgacgcaga gctcacgtcg cgacttcccg cgaatctcga tctccacgtc gggctgcgct
     2521 gggaagattt gttccggctc caggcatacg acgcgcgggg ctacggttcg aaatttccga
     2581 gcgcggggat tcccgaacag cagctgcgag tccgcgggtt ttacgaccaa tcgtggcccg
     2641 cgcgcttccg cacgatgggg gcgtgggcgg gcatcgagca aaccgagcgc gatttcccac
     2701 tgaacgttgg cgctcgcatc ggcttcgaga ctgcgtcgct gccggatgac aggacgtcgc
     2761 cgatgacgat cgcgccgatg tcggccacgg tcgacacggg actgcaatac cgcatcaccc
     2821 gcgatcgcga tgtgataccg caggtctatc tgctcgtaac gtacgggctg cagtacttcc
     2881 cgcgcgtgca cgtcaccgac agcgcgttcg atccacgcga cgcgattgcc tgctacgact
     2941 cgaaccacga ctattcgacc aacgcctgcg aagcagtccg taacggctat gccatcgaca
     3001 ccggcgtcgg tgattaccac cggatccagc aagcagcgcg gatcgcagtt cgtctgacct
     3061 ggtgacctca tgaacgcgcc gcccatcgtg gtgtacggag caaccggcct gatcggcggg
     3121 cgcgtgtgcg cagcgctcga cgccgtggac gtaccgttca tcgcggcagg tcggagacgc
     3181 gccgcgctcg agaaagtgtc gacgctcgtc ggcgccaccg actggcgcgt ggccgagctc
     3241 gagcacgacg cgctcgtcgc cgcgttcaac ggtgccgccg tcgtcgtcaa ctgcgcgggt
     3301 ccgctcgccg aggtcgcaga gcccgtgctg ctcgcgacgc tcgccgcggg cgctcattat
     3361 gtcgacctcg gcggcgatca gctcgcgatc catgcgttgt tcgagcgtca cgacgcagcc
     3421 gcgcgcaaag ccgggcgcgt cgtcgtgccg ggatgcgggc tcaactgcgc gatcggcgac
     3481 tgggcgagcg cgtgggccgc gatgaacgta tgcggcgtgc acgacgaagg cgacatcgtg
     3541 cggcatgcgc ccgcaacgcg gctcgcggaa gacaagccgc tcgacgagat cgcggtcagc
     3601 tacatcttcg acgatctcgt gctgtcgccg agcagccaga aggcggtgtt cggcaatctc
     3661 cacagccgcg gtctcgtgtg gcaccgcgat cgttgggagc acgtcgcgca ggcggcggag
     3721 cggcgcaagg tcaatgcggg tcccgcgatg ggaggccagc gcgacgcggt gtcgttcccc
     3781 ggcggcgacg tcatcacggt gccgcgtcac gtcgctgcgc gctcggtgca gacgtttgta
     3841 tcgacgacac gcagcacggc cgcgactgcg gcgttgcgtt tgctcgcgcg cgcgatgcca
     3901 ctggtgccga agcgcgcgac cgaattgctc gcaccgtatc agccgcacga ggaggattac
     3961 gcgcgtacgc ggtttgccgt gatcgcgcaa gcgagacgcg gcttcgctgc ggcgcaggtc
     4021 gtcgtcagcg gcgacgatca gtatcgtgcg agtgctggta tcgctgcatg ggtcgcacgc
     4081 acgctcgtgt cgcgcggcgc aggaccgatc ggcatgcgtg caccgagcga gctgttccgc
     4141 ccagagccgg cgctgcgcca agtcggtgaa acgatcgggc tcagcatcga gccgagtttc
     4201 ggctgacgtc cgcaccggct gctgacgtgg tatcaacgat caatgtcgat gtcgtcgtgg
     4261 caactcgagg ccgaagctcc gaccggccgc ggtgtcacgg ttcgtcgtta tcgcatgggc
     4321 aacggcctcg gcttgatcgc tgcgatcgat cgtcgcgcgc cgatcgtcgc gctgcaaacc
     4381 tggtatcgcg tcggctcgcg ccacgagcga ccgggcgcga ccggcatggc gcacctgttc
     4441 gagcatctga tgttcggcca gaccgagaag ctgccacccg gcgagttcga tcggctcgtc
     4501 gagcgcaccg gcggcgagtc gaacgccgcg acctgggtcg actggacgta ctaccggttg
     4561 tccctgcccg cgcgcgatct cgagctcggc atcaagctcg aatccgagcg catgcagcac
     4621 ctcgtgctcg aaccgacgcc ggtcgaagcc gagcgcgacg tcgtgacgaa cgaacgccgc
     4681 gagcgcgtcg aagatgacgt cgacggctgg ctcgacgagc agctgatggc gcatgcgttc
     4741 accgagcatc cgtatcgttg gccgacgatc ggctggatgg aagacatccg cgcgctcgcg
     4801 ctgcccgata tccgcgcgtt ctatcgcacg tggtacgcgc cgaacaacgc gacgatcgtg
     4861 tgcgtcggcg acttcgacga cgaaaagctg cgcgagctaa tcgcgcagca ctacggccac
     4921 atcgagccgg cgcaactgcc agacgtggcg cgaatcgtcg agcccgagca aaccaaggag
     4981 cggatcgtgc gtgcgccgaa gccgatcgcg accgaccggt tgctcgtcgg ctacaaggcg
     5041 cccggccagg atgacccgga ctgggccgcg ctcgagatcg tgtcgacgct gctcgccggc
     5101 tgtccgtcgg cgcggctgca ccgcaagctc gtgatcgaga aggaagccgc atcgtcggtc
     5161 gatgcgcagc tgacgccgtt tcgcgatccg tcgctgctcc gcctcgcggt cacgactgcg
     5221 cgcggccaca acgccgacga gatcctcgag acgatcgatc acgagatcgc agcgctcgtc
     5281 gagacgccac cgagtgcaaa ggaagtcgag aaggccaagg cgctcgcgga aacggatttc
     5341 tggagctcgc tcgtcgacgt cgacggcaag gccgaggcgt tcgggcacta cgagactgcg
     5401 ctcggcgact tccgcaagct caccgtgatc gccgagcggc tcacgcaagt caccgccgac
     5461 gatgtcgcgc gtgtcgtgcg cacgtacctg atgccgatga agcgcacgat cgtgatcgcc
     5521 gagcccgaga gcgacgaagg ggacgaggag tgagcagtcc cgttgttatc gtcgaggcgt
     5581 cacccgatac gccgctcgtc tggttcgatg tcgcgattcg cggcggcgcg gcagcagacc
     5641 ccgttggcgt cgagggcctg catcgccacg cagcattgct cgcacgccgt ggtgccggct
     5701 cgcgcgaccg ccacgcgctc gacgacacgc tcgattcgct cggcgccgcc gtcgatatct
     5761 cggtgagccg cgatgcggtc acgctgtcgg gcctgtcact gtcacgacac ctcgatgccg
     5821 tgatcgatct ctgcgccgac atcctcgcgg aaccgcggct gtcggaagac gagcacgcgc
     5881 gtctgttgcg cgaaacgccg caggtgctcg acgagatccg cgacgacgat agcgcgctcg
     5941 cgacgcgctg gtttgactgg gtgtgctcgc ccggtcaccc gtatggccgc acgtcgctcg
     6001 gcaccgagag ctcgctcgaa aagatctcgc ggcccgcggc gaccgagttg tggaagcgcg
     6061 aggtcgtctc gaacaacatg gtgatcggtc tcgccggcga catcgacgac gcgagcgcca
     6121 cacggatcgt acagcgactc accgagcgca tcccgacggg cgcgaaagct ccgtacaagg
     6181 aagaccgcag cgacgagacc ggggcgaaac gcggtcgtcg cgtgatcctc gtcaacaaat
     6241 cggatcgcac gcaggcgcaa ctgcgcatcg gtcatctcgc gctgcgctat ggccatcccg
     6301 acaccgccgc gctcgcactc gccgaggcga tattcggcgg catgttcagc tcgcggctga
     6361 tgcaggagat tcgcgtcaag cgcggctgga gctacggtgc cggctgcgcg ctgcgccgct
     6421 ctcgcctgcc ccactggttc gagatctgga cggccgcagc gatcgacgtc gccggcccgg
     6481 cggtcggcct catactcgag ctgctcgccg actacgcggc gaacggcccg accgacgacg
     6541 aagtcgattt cgcgcgcagc tatctgatcg gcgcgatgcc gtttcacgtc gcgaccgcgc
     6601 gccagcgcat gcagctcgcg gtgcgcgatg cggtgttcga gctgcccgct ggctacaccg
     6661 cggatctgcc gaaggcgctc ggccagctga ccgccgacga cgttcgcgcc gcgtgcaagc
     6721 gccacctgcg gcccgaagac gcggtgaccg tcgcggtgac gacggccgag aatgcgcagc
     6781 aggcactcgc cgctgccggc gccggtccga tgacgatcgt cgatcacgac gaatactgat
     6841 gcagcgacgg ctccgagcag gtctacggac cgccagtgat cgcgtcgaca tcggcgaggc
     6901 actggtcgtc gaatagcagg gacgagccgg tgagcacttt ctgctgcgcg aaccgcgcgg
     6961 catcctgcga gccgtcgttc tgccagaaca tgacgcagtt ctgatcgttg cagtgtgcgc
     7021 catgcggtgc gtccttgtgt ggcgcgacca tcggaatgcc gttatcgacg agcccgatcg
     7081 agtgcgcgag ctcgtggacc agcgtcgact gctcgacata gcggacgacg ttcggcgcga
     7141 gcacgatgtt cgtcgtcttg atcacgtcct tgaacatcgt gatcgtgttg ccgatcgaga
     7201 tgccgagcac gccgtactgc acaccattct gatcggcgaa gtggccgctg acgaagatca
     7261 cgtaatacga gcgccgggtt tcggtatcga acgtcgtgcg gtgctgcgcg gcgagcgcga
     7321 agatgtcctc gacggtgatc tcttcgtcgg
//
LOCUS       DASUFX010000687         5644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1359854, whole genome shotgun sequence.
ACCESSION   DASUFX010000687 DASUFX010000000
VERSION     DASUFX010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359854"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VFV99_27710"
     CDS             <1..1774
                     /locus_tag="VFV99_27710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX5063206.1"
                     /translation="GKRVVVSTGTRALQDQIARHDIPLLRTILARPFSAVVLKGVANY
                     VCRRRLAEAMSRGANESLTALLEWSESSETGDRAEVEWLAESAPLWTDVTTTPDARIG
                     PRCPYFERCFVTQARRLAEKAELILVNHHLYFADRALRAAHPGARVLPEHDAVIFDEA
                     HQLEDVATEHFGARVSTFSLAQLVRDAHLSLARMPLWTGRAADDTIAAVERSGLSLFA
                     MIRSALITENESRMPLPASLFDQPERQTAWFKLDNALEDLARVAEAEAEPPPDGEETE
                     DAGARATLNGLARRAREMRDALAVIAEQRQRSHVYWGEVRPSGTLLTASPIEVGDLVR
                     RHIVSAGPTPIFTSATLAAAGDFSYQRARLGLDEPEACSELLVPSPFDYAKQAMLYVP
                     RDLPPPADDSFSVTAAQRTLELLEITNGRAFLLFTSHRALREAATRLASAPYPRLVQG
                     DAPRATLIDRFRATPGAILLGTSSFWEGIDVPGDALSLVVIDKLPFSPHTDPLIAARM
                     QACAEANQDPFQTIQLPAAAIALKQGFGRLIRRRDDRGIVAILDSRIVTRTYGRVFIE
                     TLPSGLPRTSALEQVKRWWQTPPS"
     gene            1795..2343
                     /gene="plsY"
                     /locus_tag="VFV99_27715"
     CDS             1795..2343
                     /gene="plsY"
                     /locus_tag="VFV99_27715"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5063207.1"
                     /translation="MILAGFFAGSVPFGVIVAKQRGVDIRNKGSGNIGATNVGRVLGV
                     KDGLLVLVLDSMKGWLITFAASRFDGDPWVVVATGFAAVLGHCFSPWLGFKGGKGVAT
                     ALGVFIVVSPALALVAVSVFLVVAGRTRIPALGSLGAVTSATIYAFVTSAPTPVELLA
                     LATSLLLVYTHRVNLARLYHED"
     gene            complement(2328..2837)
                     /gene="folK"
                     /locus_tag="VFV99_27720"
     CDS             complement(2328..2837)
                     /gene="folK"
                     /locus_tag="VFV99_27720"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5063208.1"
                     /translation="MLADDALVIGLGGNVGGEPAIIERFVRAREAFAQLGDVKSAALY
                     RTAPIGPAQASFLNSAIRVRWRGAIASEVMATVLEIERLLGRDRRGEVRWGPRTIDLD
                     VLVWGSRVILTPELEVPHPRLGERRFALQPLVDLVGAETILPGSHDTLGALVQRVAAQ
                     ELELLQSSW"
     gene            complement(2840..3454)
                     /locus_tag="VFV99_27725"
     CDS             complement(2840..3454)
                     /locus_tag="VFV99_27725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5063209.1"
                     /translation="MLRPSKIIGIGQNYRAHAAEMGKGIPEEPLMFLKPPTAIIADGM
                     PIHRPPGYERVDYEGELGVVIGKRAHRVTREQALEYVKGYVCVNDVTVRDLQKKDGQW
                     TRAKGFDTFCPMGPRIVPGLDPTNLRITTRVNGVVKQDSSTSDLIFDVATLISFCSQH
                     MTLEEGDVISTGTPSGVGPLQPGDVVEVEIEGIGVLTNPVIARP"
     gene            3578..>5644
                     /locus_tag="VFV99_27730"
     CDS             3578..>5644
                     /locus_tag="VFV99_27730"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5063210.1"
                     /translation="MPRAKPFPDSEEEEKTTIESQWEDEASTTVEQGDVADKIRSLVE
                     PRREITSTGAGPVDEPTVDDQHAQMSAITPVRDVARLAITQGNDAGTEIEIRPGKAYT
                     IGRAIDNDVVLTDIAVSRKHFDLRFEDAAWVIVDRGSGNGTVVNGNLEDNPFMLANGD
                     VIEIGNTVFRFDQPNGASRPQPTVDLDADDEEMSTVAGKPMRGDRDVEPMPAPVAARP
                     PRPKTLPPPTPLRPSGSTQPPPLGFPPPGPAPLAQPASTMPMAQMANRPPLGSPNLPG
                     VAPMAPAMLGDQRGFPNLPNPSPLGSQQLPTTIPGQGAPPPRQQQMQMYPYPQATEIP
                     PHSVHAQMLLIQTQNRRGDHSTAHVPPMSFDQLPQQRYSQPQLTKKAKVVLIGVGLAV
                     FAAVMTVAIVKSNSGKPQASAATTGSNDNVKLESKTEAKVAAKTTPVEETPEPAKPEP
                     AKTAVTTTPATTPAAPTAKAEPAKTSPPPPTAIAKTEPVKTPPPPPVKTEPVKTEPVK
                     TEPVKVVKTEPKKTEAKKTEPKTTKRPEKRTEARRETQVAVATPPEPKKTGGNPDAAR
                     SEAERLYRAKKFSDASTYLANQAKKFDEDIAKDMRRTSEYYAKLGRAFAQGTAPATRA
                     VDAFESLRSAQNYDRNLSNAFDAEIQTKLAQVAPKAALSYVAAKNYPAARSAMLFAKQ
                     FPSATES"
BASE COUNT          995 a         1931 c         1857 g          861 t
ORIGIN      
        1 gggcaagcgc gtcgtggtgt cgacggggac gcgcgcactg caggatcaga tcgcgcgcca
       61 cgacattccg ctcctccgca cgatcttggc gcgaccgttc tcggcggtcg tgctcaaggg
      121 cgtggcgaac tacgtgtgtc ggcggcggct cgccgaggca atgtcgcgcg gcgcgaacga
      181 atcgctgaca gcgctcctgg aatggagtga gagctcggag accggcgatc gtgccgaggt
      241 cgagtggctc gccgaatcgg cgccgctctg gaccgacgtg acgacaacac cggatgcgcg
      301 catcgggccg cgctgcccgt acttcgagcg ctgcttcgtg acgcaagcgc gccggctcgc
      361 cgagaaggcc gagctgatcc tcgtgaacca tcacctgtac ttcgcggacc gcgcgctgcg
      421 tgccgcgcat ccgggcgcgc gcgtgctgcc ggagcacgat gcggtgatct tcgacgaggc
      481 acaccagctc gaagacgttg cgaccgagca cttcggcgca cgcgtgtcga cgttctcgct
      541 ggcgcagctc gtgcgcgacg cgcatctgtc gctcgcgcga atgccgctgt ggaccgggcg
      601 tgccgccgac gacacgatcg ccgcggtcga gcgctctggt ctctcgttgt tcgcgatgat
      661 ccgcagcgcg ctgatcaccg agaacgagtc gcgcatgccg ttgccagcga gcttgttcga
      721 tcagccggag cgccagaccg catggttcaa gctcgacaat gccctcgagg atctcgcccg
      781 ggtcgcggag gccgaggccg agccaccgcc cgatggcgaa gagaccgagg acgcaggtgc
      841 gcgtgcgacc ctcaacggct tggcgcggcg agcgcgcgag atgcgcgatg cgctcgcggt
      901 gatcgcggag cagcgccagc gcagccatgt ctactggggc gaggtgaggc cgagcggcac
      961 gctgctgacc gcatcgccga tcgaggtggg cgacctcgtg cgcaggcata tcgtgagcgc
     1021 aggaccgacg ccgatcttca cgagcgcgac gctggccgcc gcgggtgact tcagctacca
     1081 gcgcgcgcgg ctcggtctcg acgagccaga ggcgtgtagc gagctgctcg taccgtcgcc
     1141 gttcgactat gcgaagcagg cgatgctcta cgtgccacgc gacctgccgc cgcccgccga
     1201 cgactcgttc tcggtgacag ctgcgcagcg cacactcgag ctcctcgaga tcacgaacgg
     1261 ccgcgcgttc ctgttgttca cgtcgcatcg cgcgctacgc gaagccgcga cccgactcgc
     1321 cagcgcgccg tatccgcggc tcgtgcaagg cgatgcgccg cgcgcgacgt tgatcgatcg
     1381 cttccgcgca acacccggcg ccatcctctt gggcacgagc tcgttctggg agggcatcga
     1441 tgtccccggc gatgcgctca gcctcgtggt catcgacaag ctcccatttt ccccacacac
     1501 cgatccattg attgcggcgc gcatgcaggc gtgcgccgag gcgaaccagg atccgttcca
     1561 gaccatccag ctgcccgccg cagcgattgc gctcaagcag ggcttcggcc gcctcattcg
     1621 ccgccgcgat gaccgcggga ttgtcgcgat tctggattcg aggatcgtga cgcgcaccta
     1681 cggccgcgtg tttatcgaga ccttgccgag tgggctccca cgcacatccg cgctcgagca
     1741 agttaagcgt tggtggcaga cgccaccgag ctagacttgg ctgtggtcgt cttgatgatt
     1801 ctcgccggct tctttgcggg atcggtcccg ttcggcgtga tcgtcgcgaa gcagcgcgga
     1861 gtcgacatcc gcaacaaagg cagtggcaac atcggcgcca ccaacgtcgg ccgcgtgctc
     1921 ggcgtcaagg acggcttgct cgtgctggtc ctcgactcga tgaagggctg gctcatcacg
     1981 ttcgccgctt cgcggttcga tggcgatccg tgggtcgtgg tcgcgaccgg atttgccgcg
     2041 gtcctcggtc actgcttctc gccgtggctc ggcttcaagg gtggcaaggg cgtcgccacc
     2101 gcactgggcg tgttcatcgt cgtgtcgccc gcactcgcgc tggtcgcggt gtccgtgttc
     2161 ctcgtcgtcg ccggccgcac gcggattcca gcgctcggct cgctcggcgc ggtcacctcg
     2221 gcgacgatct acgcattcgt gacgagcgca ccgacgccgg tcgagctact cgcgctcgcg
     2281 acgtcgctgc tgcttgtcta cacgcatcgc gtgaacctgg cgcggctcta ccacgaggac
     2341 tgaagcagct cgagctcctg ggcagcgacg cgctgcacga gcgcgccgag cgtgtcgtgc
     2401 gagccgggaa gaatcgtctc cgcaccgacg agatcgacga gcggctgcag cgcgaaccgt
     2461 cgttcgccga gacgcggatg cggcacctcg agctcgggtg taagtatcac gcgcgagccc
     2521 cacacgagca cgtcgagatc gatcgtgcgc ggtccccagc gtacctcgcc gcgccgatcg
     2581 cgcccgagca gccgctcgat ctcgagcacg gtcgccatca cctcgctcgc gatcgcacct
     2641 cgccagcgca cgcggatcgc cgagttcaga aacgacgcct gcgcgggtcc gatcggtgcg
     2701 gtgcgataga gcgccgcgct cttgacgtcg ccgagctgcg cgaacgcttc gcgtgcacga
     2761 acgaagcgct cgatgatcgc aggctcgccg ccgacgttgc cgccgagccc gatcacgagc
     2821 gcgtcgtcag cgagcatcgt tacggccgag cgatcaccgg attggtcaac acgccgatgc
     2881 cctcgatctc gacctcgacg acgtcaccgg gctgaagcgg accgacgccg ctcggcgtac
     2941 cggtcgagat cacgtcgcct tcttcgagcg tcatgtgctg actgcagaac gagatcagcg
     3001 tcgcgacatc gaagatcaga tcgctggtcg acgagtcttg tttgacgacg ccgttgacgc
     3061 gcgtcgtgat gcgcaggttc gtcggatcga gaccggggac gatgcgtggc cccatggggc
     3121 aaaaggtgtc gaaccctttc gcgcgcgtcc actgcccgtc ttttttctgt agatcgcgca
     3181 cggtcacgtc gttcacgcag acgtacccct tcacgtattc gagcgcctgc tcccgcgtga
     3241 cgcggtgcgc gcgcttgccg atcacgacgc cgagctcgcc ctcgtaatcg acgcgttcgt
     3301 acccaggcgg gcgatgaatc ggcatgccat cggcgatgat tgccgtcggt ggcttcaaga
     3361 acatgagcgg ttcctcgggg atccctttgc ccatctccgc agcgtgtgcg cggtagttct
     3421 ggccgatgcc gatgatcttg ctggggcgaa gcatggcggg aaaggtagga cacctgagcc
     3481 gccgtgtcat tgatcgatac cgttgcgccc cgtacaattt acggcgatgg gccgggctcc
     3541 aaatcctgga gggccacaac gtgtaaccgc ccacgttgtg ccgagggcga agcctttccc
     3601 cgacagcgaa gaagaagaga aaacgaccat cgagtcgcag tgggaagacg aagcgagcac
     3661 gacggtcgag cagggtgatg tcgcggacaa gattcgatcg ctcgtcgaac cgcgccgcga
     3721 gatcacgagc accggcgccg gaccggtcga tgaaccgacg gtagacgatc agcacgcgca
     3781 gatgtctgcg atcacgccgg ttcgcgatgt cgcgcgcctg gcgatcacgc aaggtaacga
     3841 cgccggcacc gagatcgaga ttcgtccggg caaggcgtac acgatcggcc gcgcgatcga
     3901 caacgacgtc gtgctcaccg acatcgcggt ctcgcgcaag cacttcgatc tgcgcttcga
     3961 agatgccgcg tgggtgatcg tcgatcgcgg ctcgggcaac ggcaccgtcg tcaacggcaa
     4021 cctcgaagac aacccgttca tgctcgcgaa cggagacgtg atcgaaatcg gcaacaccgt
     4081 gttccgcttc gatcagccaa acggtgcctc gcgcccgcag cccaccgtcg atctcgacgc
     4141 ggacgacgaa gagatgtcga cggtcgcggg caagccgatg cgcggtgacc gcgatgtcga
     4201 gccgatgccg gcgcccgttg cagcgcgccc gccgcgcccg aagacgttgc cgccaccgac
     4261 gccactgcga ccgtcgggct cgacgcaacc gccaccgctc ggctttccgc cgccaggccc
     4321 ggcgccgctc gcgcaacctg cgtcgacgat gccgatggcg cagatggcga atcgtccgcc
     4381 gctcggcagt ccgaacctcc ccggcgtcgc gccgatggcg ccggcaatgc tcggcgatca
     4441 gcggggcttc ccgaacctgc ccaatccttc gccgctcggc tcgcagcagc tgccgacgac
     4501 gattcctggc caaggcgcgc cgcctccgcg ccagcagcag atgcagatgt atccgtatcc
     4561 gcaggcgacg gagatcccgc cgcactcggt gcatgcacag atgctgctga tccagacgca
     4621 gaatcgccgc ggcgatcact cgacggcaca cgtgccgccg atgtcgttcg atcagctgcc
     4681 gcagcagcgt tactcgcagc ctcagctcac gaagaaggcc aaggtcgtgc tcatcggcgt
     4741 cggcctcgcg gtgttcgccg cggtgatgac cgtcgcgatc gtgaagtcga acagcggcaa
     4801 gccacaggcg agcgcggcga cgactggctc caacgacaac gtcaagctcg agagcaagac
     4861 cgaagcaaag gtcgcggcca agacgacgcc ggtcgaagag acgcccgagc ctgcgaagcc
     4921 ggagccggcc aagacggccg tgaccacgac gcccgcgacg acgcccgcag cgccgactgc
     4981 gaaggcggag cccgccaaga cgtccccgcc accgccgacg gcgatcgcga agaccgagcc
     5041 ggtcaagacg ccgccgccgc cgcccgtgaa gaccgaaccg gtcaagaccg agcccgtcaa
     5101 gaccgagccc gtcaaggtcg tgaagacgga gccgaagaag acggaagcca agaagaccga
     5161 acccaagacg acgaagcgtc ccgagaagcg caccgaagcg cgccgcgaga cgcaggtcgc
     5221 cgtcgcgacg ccgcccgagc cgaagaagac cggtggcaac ccggacgccg cgcgctccga
     5281 ggcagagcgt ctgtaccgcg cgaagaagtt cagcgacgca tcgacgtacc tggcgaacca
     5341 ggcaaagaag ttcgacgagg atattgcgaa ggacatgcgg cggacgtccg agtactacgc
     5401 gaagctcggg cgcgcgttcg cacaaggcac cgcgccggcg acacgcgccg tcgacgcgtt
     5461 cgagtcgctg cgttcggctc agaactacga tcgcaacctc agcaacgcgt tcgatgccga
     5521 gatccagacc aagctcgcgc aggtcgcgcc caaggccgcg ctgtcgtacg tcgccgcgaa
     5581 gaactatccg gcagcacgca gcgcgatgct gttcgcaaag cagttcccga gcgcgacgga
     5641 gagc
//
LOCUS       DASUFX010000688        10128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1915183, whole genome shotgun sequence.
ACCESSION   DASUFX010000688 DASUFX010000000
VERSION     DASUFX010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915183"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFV99_27735"
     CDS             <1..361
                     /locus_tag="VFV99_27735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063211.1"
                     /translation="LRRLDDDRWAVSPINDLYRRALTRNGRLAHVVETNAPEVILASE
                     RHELHTAILHLFDGEIALRTICGGNEGFVEALLELDRHTPGNEMTGKLYRAEAVLFGL
                     AFTRDSVPNDATREAAA"
     gene            358..999
                     /locus_tag="VFV99_27740"
     CDS             358..999
                     /locus_tag="VFV99_27740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063212.1"
                     /translation="MNPYEIIEAWHSRVHGALADALAYLATLEAAPVSDGILSEWVDV
                     LVAMKFDDTDIPLIVHIIDKSDPHLQEAGVRLARAAFRTARGTEALEPPLARLVSQPI
                     DPWVLEALVDLLADTRAELTTVYQALAQIHGQPSRREWGIMRNRAIQPWRRLVQVYLQ
                     HAIQVAHPRERELAFLPVLERELGTRGWDVTQRHILTSMGFDPDEHLRLLRGR"
     gene            complement(996..1394)
                     /locus_tag="VFV99_27745"
     CDS             complement(996..1394)
                     /locus_tag="VFV99_27745"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5063213.1"
                     /translation="MRCPNCKSVDSFRPWEGTMKLRGVEFLARGEKCRECGEIVFDSS
                     EVRRQERVVAAGLVERGIRTGRDFQYVRKVAGLKATELAELLDVRPETVSRWERDEVD
                     IPRPIAFTLGELFERPRILRDRLEAFAHRR"
     gene            complement(1402..1650)
                     /locus_tag="VFV99_27750"
     CDS             complement(1402..1650)
                     /locus_tag="VFV99_27750"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX5063214.1"
                     /translation="MLARIKELVAKGHVRYTFHARMRMDERNASESDVCSALLSASAA
                     DYNAAKDNYRVSGGADQDGDPMTVVVSVEADVVVITIF"
     gene            complement(1749..3482)
                     /gene="recN"
                     /locus_tag="VFV99_27755"
     CDS             complement(1749..3482)
                     /gene="recN"
                     /locus_tag="VFV99_27755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5063215.1"
                     /translation="MLRHLRATNFAILSDVEIELGLGMNVLTGETGAGKSLIVEAVNL
                     LRGGRASADIPRTGTDEAVVEATVEVPSDLAARVADVLDGAGLPNEGSEVFIRRVIQR
                     GGRSRCYVNGALTTAARLGELGALLIDLSGQHQHQGLVDPGRHLEILDLFSGGAAEVA
                     AMQTAWAELKRCDEAVLALGGDEREREARIDYVRYQLEELDAANLQTGEDQSIEIERA
                     RLASVDQLQGAARTAEELLYGGDDSARDRVAAAAREVERAVRTDPTLDGVVRQLGEIE
                     TLIDDAADQLRGYADRLEGDPERLAYLDERLALIRRLTRKHGATLDEVIAKAAELRGE
                     LAALTGRDEKLAEAQAARKQAETVAVAAAAALTKTRTKAARKLEKEVNAALADLGMAS
                     AKLSVVIEPRAAAAGERAGLGPTGADRIELMLAANQGEDRRPLTKVASGGELSRIMLA
                     LKLSLRSADEVATYVFDEVDTGIGGSTAQVVGAQIRSVADHRQVLCVTHLPQIAAYAD
                     HHFHVEKTELAGRTETHVRKLTGTARKDELARMLGGQATSKAKAHAAELLAEAARTDS
                     RSDAKKPRARA"
     gene            complement(3482..4357)
                     /locus_tag="VFV99_27760"
     CDS             complement(3482..4357)
                     /locus_tag="VFV99_27760"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX5063216.1"
                     /translation="MRVGFILKPDKTEAGVLLEELVPWLRDQGHVPVVPTEDQIAPAG
                     TEIVPEEELGDAVDLAVVLGGDGTMLRASRLVADQGKPVLGVNLGQLGFLAMFSPAEA
                     KTALAAALAGKLPRAERMRLQVTFTRAGTSPDSGVVKFALNDAVLHQGAMARLIEVNA
                     YCDGQLIAAYRADGLIIATPTGSTAYSMAAGGPIVVPAQAAMTLTPICAHALTNRPLV
                     LSATSTIRLELGVDARGVILTVDGQWAHSFLPGDVVEMTAAKRPLVMFVSQQSFFDVM
                     RDKLHWGVRLDKGAK"
     gene            4476..4925
                     /gene="rplM"
                     /locus_tag="VFV99_27765"
     CDS             4476..4925
                     /gene="rplM"
                     /locus_tag="VFV99_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5063217.1"
                     /translation="MMTQKSFWLTKEAGEAQREWYVVDATGQTLGRLASKIAMVLRGK
                     HKATYTPNVDMGDFVIVLNAGGVRLTGTKPEKKLYQHHTLYPGGIRTRIAKDVLADDP
                     ERAIREAVWGMMPKGPLGRRIIKKLKIYKGSEHEHTAQKPRVLEIKN"
     gene            4938..5333
                     /gene="rpsI"
                     /locus_tag="VFV99_27770"
     CDS             4938..5333
                     /gene="rpsI"
                     /locus_tag="VFV99_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5063218.1"
                     /translation="MTTAQKTYATGRRKTAVARVWLTSGEGKFTINDRSDEDYFSRPT
                     ARMVIRQALEEVALLDKYDVHATVVGGGIASQAGAIRHGITRALMKTDSDLRPKLKAA
                     GYVTRDPRKKERKKYGQKAARARFQFSKR"
     gene            5574..8864
                     /locus_tag="VFV99_27775"
     CDS             5574..8864
                     /locus_tag="VFV99_27775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063219.1"
                     /translation="MGGSATATGAKLGRYQIVKHLASGGMAEVLLARATGIEGFTRHV
                     VIKRIRPGQAKDQTFVQMFLNEARIAAQLHHANIVQVHDIGQEGNEYFFAMEYVHGED
                     VRRLLTNLAKHRQKLPFEHVVTIITNAAIALHHAHDQRGPDRKPLGIVHCDVTPANIL
                     VGYDGNVKVVDFGIAKAAAKTEETQAGTLKGKASYMSPEQCTGSKVDRRSDVFALGIV
                     LYEVVTTRRLFKAENDFLTMSAIVAGKIPSAKQYRPDLPDALEAILLKALALKVEDRY
                     QTADQLREALEQFAANAGLRTSTSALAAYMKQQFGERPEPWLVDDDEPEMELVVDFDG
                     SQSALVSPPQESLHELAIPTDVEALNSSPIVKARTKEISKTPATTPAPPKRQMPLAPP
                     GPLPKQAKLPTAQIPTLGVPPASDKSRPFRIDTGPVPAPVPRKKPITVQIPVVNQAAG
                     VAPKRVESPGGLPGVATKKSDGVPQAADASDSAATFVSGAVDNVDGAWDVSQAFVAAT
                     SKAAGVNALPVDGKASDPKGEAKPALDTKAATKPADAKRESKPADTKASDAKVENKPK
                     AKPAAGTPPPAMPPRKTPPIPFANVGGAQPVPSVKSKPSDAVPGAAKPTDTQPTRVAP
                     KPGDSQPIESTAIDSRSAPPLAAKPTDTQPTRVAPKPSDSQPLDSTAIDSRPALPLAP
                     ADSQPIDATAIDSRPAGARPVTPSPVAPAFQTGGTPTPTPPTPTPTPTPLPPVFAPTD
                     TTPPPPVFAHATTPLPPKLPPAPEPKTWSLAELASMKGEKPVDKNDASTTSKFDVGSL
                     STSRTDASNVPVPYAPTAHAASPADAIAAADAAADKVKRPGTDGRDSAPSLPPPPAAR
                     VKLPGVAQTGEAAPGTLASKFAAPNVFTPGRTKTPSTVPDTWAGGTSTGKALSPLRRL
                     LRSPIGLALVAGALVTVVVVIVFVTRGSKPTPAKATPPSEPAHVTLPAETQPAETQPA
                     ETQPNETQPTETQTPRVDQAATKPVTKPATKPTTKPATNQITKPVATKPTTVTKPTTV
                     TKPATTKPATTKPTTTKPNTQITKPATTTKPTTTKPATKPATKPATKPTKPPAKKWDP
                     NTLFPSRK"
     gene            8869..9582
                     /locus_tag="VFV99_27780"
     CDS             8869..9582
                     /locus_tag="VFV99_27780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063220.1"
                     /translation="MKHSLIVALIALPLVAFAGPKEKKEAQQHIEKATSLHKAGKYAE
                     ALTELQAAYALDAQPDLLYAIGQIYVKLDKCDEAISFYEKFIATQPPADAGNSANEAI
                     ETCKAKQPPPPPPPEPTPPPQPPPPPPQPESKPFYTDKIGTALVGGGVVAIVVGGLLY
                     SSARSTLDDADAAMTYAAHQELVDDAHLKRNISVGVGIVGLAAIGVGVWHYMQYQSEQ
                     SVAVTPTTSGGMVTWMGRF"
     gene            9582..>10128
                     /locus_tag="VFV99_27785"
     CDS             9582..>10128
                     /locus_tag="VFV99_27785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX5063221.1"
                     /translation="MRAVFSLFALVALAGSSAGCLRTTEFKCTSDADCSASGAVCEST
                     SYCSFTDTDCAMGRRYGELSGPYSNQCVGETSMNDGGIDGPDGGTGNCPATYAALPGA
                     GGHVYKLTNNSQQWSTQRDRCANEGAYLAIPDDANELMAITTAAAAAKTWIGISDTAM
                     EMTYVTVKMTPATFLPWASGEP"
BASE COUNT         1847 a         3538 c         3233 g         1510 t
ORIGIN      
        1 cctgcgccgg ctcgacgatg atcgctgggc ggtgtcgccg atcaacgatc tctatcgtcg
       61 cgcgctcacg cgcaacggcc ggctggcgca cgtcgtcgag accaacgcac ccgaggtgat
      121 cctcgcgagc gagcgccacg agctccacac ggcgatcctc cacttgttcg acggcgaaat
      181 cgccttgcga acgatctgtg gcggcaacga aggctttgtc gaggcgctac tcgagctcga
      241 ccgccacacg cccggcaacg agatgaccgg caagctgtat cgcgccgaag cggtgctgtt
      301 cgggctcgcg ttcacacgcg acagcgttcc gaacgacgcc acgcgcgagg cagccgcgtg
      361 aacccgtacg agatcatcga ggcgtggcac tcgcgcgtcc acggcgcact cgccgatgcc
      421 ctcgcctacc tcgccactct cgaggcagcg ccggtctctg acggcatcct gagcgaatgg
      481 gtcgacgtcc tcgtcgcgat gaagttcgac gacacggaca tcccgctcat cgtgcacatc
      541 atcgacaagt ccgatccgca cctgcaggaa gcgggcgtac gcctcgcgcg tgccgcattc
      601 cgcaccgcgc gcggaaccga agcgctcgag ccaccactcg cgcgcctcgt gtcgcagccg
      661 atcgatccgt gggtgctcga agcgctcgtc gatttactcg cggacacgcg cgccgagctg
      721 acgaccgtct atcaagccct cgcgcagatt cacggacagc cgtcgcgccg cgaatggggc
      781 atcatgcgca accgcgccat ccagccgtgg cgccgtctcg ttcaggtcta tctccagcac
      841 gcgatccagg tcgcgcatcc gcgcgagcgc gagcttgcct tcctcccggt gctagagcgc
      901 gagctcggca cgcgcggctg ggacgtcacg cagcggcaca tcctgacgag catgggcttc
      961 gatcccgacg agcacctgcg cctattacgc ggtcgctagc gacggtgtgc gaatgcctcg
     1021 agcctgtcgc gcaggatgcg tggacgctcg aagagctcac ctagcgtgaa tgcgatcggg
     1081 cgcggaatgt cgacctcgtc gcgctcccac cgcgagacgg tctcgggcct gacatcgagc
     1141 agctcggcta gctcagtcgc cttcaggcca gcgactttac gcacgtactg gaagtccctg
     1201 cccgtgcgaa taccgcgctc caccaagccc gcggccacaa ctcgctcctg acggcgaacc
     1261 tcgctgctgt cgaacacgat ctcgccgcac tcgcggcact tctcaccgcg cgcgagaaac
     1321 tcgacgccgc gcagcttcat cgtgccttcc cagggacgga acgaatcgac actcttgcag
     1381 ttggggcagc gcatgggcat ctcagaagat cgtgatgacg acgacatcgg cttcgaccga
     1441 gacaacgacc gtcatcggat cgccgtcctg atcggcgccg ccggacacgc ggtagttgtc
     1501 cttggctgcg ttgtagtcgg cagcgctcgc agacagaagt gcggagcaca cgtccgattc
     1561 ggacgcattg cgttcgtcca tccgcatccg cgcgtggaac gtgtagcgga cgtggccctt
     1621 cgcgaccagt tccttgatcc gcgcgagcac gtcatgtctc caggttagct ctactagaca
     1681 ttatgtcaag cgtcctcgtc ggacgatact cggacgtcca actcgtccgt ttctctcgtt
     1741 ttcgcgactc aggcgcgagc gcgtggcttc ttcgcgtcgc tgcgcgagtc ggtacgcgcg
     1801 gcttcggcaa gcagctcggc tgcgtgcgcc ttcgccttcg acgtcgcctg gccaccgagc
     1861 atccgcgcca gctcgtcctt gcgcgccgtg ccggtgagct tgcgcacgtg cgtctcggtg
     1921 cggcccgcca gctccgtctt ctcgacgtgg aagtgatgat cggcgtacgc cgcgatctgg
     1981 ggcaagtggg tcacgcagag cacctggcga tgatcggcga ccgagcgaat ctgcgcgccg
     2041 acgacctgcg ccgtcgagcc gccgatgccg gtgtcgacct cgtcgaacac gtacgtcgcg
     2101 acctcgtcgg cgcttcgcag cgacagcttg agcgcgagca tgatgcgcga gagctcgccg
     2161 cccgacgcga ccttcgtgag cggccggcga tcctcgccct ggttcgcagc caacatgagc
     2221 tcgatgcggt cggctcccgt cggacccagg ccagcgcgct cgcccgccgc ggcagcgcgc
     2281 ggctcgatca cgaccgatag cttcgccgac gccatgccga ggtcggcgag cgcggcgttg
     2341 acttccttct cgagcttgcg cgctgccttc gtgcgcgtct tcgtcagcgc tgccgcagca
     2401 gcgacggcaa ccgtctccgc ttgcttgcgc gcggcctgtg cctcggcgag cttctcgtcg
     2461 cggccggtca gtgcagcgag ctcgccgcgc agctcggctg ccttcgcgat cacctcgtcg
     2521 agcgtcgcgc cgtgcttgcg cgtgagccgg cggatcaacg cgagccgttc gtcgagatac
     2581 gcgagtcgct cgggatcacc ctcgaggcgg tcggcgtagc cgcgcagttg atcggcggca
     2641 tcgtcgatca aggtctcgat ctcgccgagc tggcgcacga caccgtcgag cgtcggatcg
     2701 gtgcgcacgg cacgttcgac ctcgcgtgca gcggccgcga cgcgatcgcg cgcgctgtcg
     2761 tcgccgccat acaagagctc ctccgccgtg cgcgccgcgc cttgcagctg atcgacactc
     2821 gcgagccggg cgcgctcgat ctcgatcgac tgatcttcgc cggtttgtag atttgccgcg
     2881 tcgagctctt cgagctggta gcgcacgtaa tcgatgcgcg cctcgcgttc gcgctcgtcg
     2941 ccaccgagcg cgagcaccgc ctcgtcgcag cgcttgagct cggcccacgc ggtttgcatc
     3001 gcggcgactt cggctgcgcc gccggaaaat agatcgagga tctcaaggtg gcgtcccgga
     3061 tcgacgaggc cctggtgctg atgctgaccc gacagatcga tcagcagtgc accgagctcg
     3121 ccgagccgtg ccgcggtggt cagcgcgccg ttgacgtagc agcggctgcg cccgccccgc
     3181 tggatgacgc ggcggataaa cacctcgctg ccttcgttcg gcaggcccgc accgtcgagc
     3241 acgtcggcaa cgcgcgccgc gagatcgctc ggcacttcga ccgtcgcctc gacgactgct
     3301 tcgtcggtgc ctgtgcgcgg aatgtcggcc gacgcgcgcc cgccgcgcag caagttcacc
     3361 gcttcgacga taagcgactt gcccgcgccc gtctcgccgg tcagcacgtt catgccgagg
     3421 ccgagctcga tctcgacgtc ggacaggatc gcgaaattcg tcgcgcgcag gtggcgaagc
     3481 attacttggc tcccttatcg agccgcacgc cccagtgcag cttgtcgcgc atgacgtcga
     3541 agaagctctg ctgcgagacg aacatgacga gcggtcgctt cgccgcggtc atctcgacga
     3601 catcgccggg caagaacgag tgtgcccact ggccgtcgac ggtgaggatg acgccgcgtg
     3661 catcgacacc tagctcgagc cggatcgtcg aagtggcgct gagcacgagc ggccggttcg
     3721 tcagcgcgtg cgcgcaaatc ggcgtgagtg tcatcgccgc ctgcgcgggc accacgatcg
     3781 gtccgcccgc ggccatgctg tacgcggtcg atccggtcgg cgtcgcgatg atgagaccgt
     3841 cggcgcgata cgccgcgatc aactgaccgt cgcagtacgc gttgacctcg atgaggcgcg
     3901 ccatcgcgcc ctggtgcagc accgcgtcgt tgagcgcgaa cttcacgacc ccggaatcgg
     3961 gcgacgtgcc ggcgcgcgtg aaggtgacct ggagccgcat gcgctcggcg cgcggcagct
     4021 tgccggcgag cgccgcggcg agcgcggttt tcgcttcggc gggcgaaaac atcgccaaaa
     4081 acccgagctg gccgaggttg accccgagca ccggttttcc ctgatcggcg acgagccggg
     4141 acgcccgcaa catcgtgcca tccccgccga ggacgaccgc caggtcgacc gcgtcgccca
     4201 gctcctcctc ggggacgatt tcggtccccg cgggagcgat ctggtcctcc gtcgggacaa
     4261 ccggtacatg tccttgatct ctcagccaag gcaccagttc ttcgagcaag accccggcct
     4321 cggtcttgtc tggcttcaga atgaacccga cgcgcacaac ctggtggtac cacagccgaa
     4381 tgacactgaa cgaatcaaca ggtcacgcgt tcgcgagtct tgacacagct ttcgcaacct
     4441 tgtagacgat ccgctccttt aggacggcac tgcaaatgat gacgcaaaag agcttctggc
     4501 tcaccaaaga ggctggcgag gcgcagcgcg agtggtacgt ggtcgatgcg actggtcaga
     4561 cgcttggtcg gctggcgagc aagatcgcca tggtcctgcg cggcaagcac aaggcgacgt
     4621 acacgccgaa tgtcgacatg ggcgatttcg tgatcgtgct caacgcgggc ggcgttcgcc
     4681 tgacgggcac gaagcccgag aagaagctct atcaacacca cacgctttac ccgggtggta
     4741 ttcgcacgcg catcgcgaag gacgtcctcg cggatgaccc cgagcgcgcg attcgcgaag
     4801 cggtgtgggg catgatgccg aagggcccgc ttggccgtcg catcatcaag aagctcaaga
     4861 tctacaaggg cagcgagcac gagcacacgg cacagaagcc gcgcgttctc gagattaaga
     4921 actaggaccg gtagcaaatg acgacagcac agaagacgta cgccacgggt cgccgcaaga
     4981 cggcagtcgc tcgcgtgtgg ctcacctcgg gcgagggcaa gttcacgatc aacgatcgca
     5041 gcgacgagga ttacttctcg cgcccgaccg cgcgcatggt cattcgccag gcgctcgaag
     5101 aggtcgcgct gctcgacaag tacgacgtcc acgcgacggt cgtcggtggt ggcatcgcct
     5161 cgcaggcggg cgcgatccgc catggcatca cgcgcgcgct gatgaagacc gacagcgacc
     5221 tccgcccgaa gctcaaggcg gcgggctacg tcacgcgcga cccgcgcaag aaggagcgta
     5281 agaagtacgg ccagaaggcc gcgcgcgctc gcttccagtt cagcaagcgc tgatcacatc
     5341 ctggcacgct gctgcttctg taacagaccc tcgcggttgc gagggtcttc gcattttcgg
     5401 ataccgcgcc cccaccccgc gtcggcgctt gtcgatctcg ccagcgccca cgtaggcgcg
     5461 ttgaccgcac gcgccacgcg atttctcgac gaaattcgac ggcacaccta cattggacct
     5521 ccgacgagcg gagcttgacg taactgcctg tgaggcgtca cgcttgttcg agcgtggggg
     5581 gttctgccac cgctacgggc gcgaagctcg gccgctatca gatcgtcaaa catctggcga
     5641 gcggcggcat ggcggaggtg ttgctcgcgc gtgcgaccgg catcgaaggg ttcacgcgtc
     5701 acgtcgtgat caagcggatc cgcccgggtc aggcgaagga ccagacgttc gtccagatgt
     5761 tcctgaacga agcgcgaatc gcagcgcagc tgcaccacgc caacatcgtt caagtccacg
     5821 acatcggcca ggaaggcaac gagtacttct tcgcgatgga gtacgtgcac ggcgaggacg
     5881 tccgccgcct gctcaccaac ctcgcgaagc atcgccagaa gctcccgttc gagcacgtcg
     5941 tcacgatcat cacgaacgcc gcgatcgcgc tgcatcacgc acacgatcag cgcggccccg
     6001 atcgcaaacc gctcggcatc gtgcattgcg acgtcacccc cgcgaacatc ctcgtcggct
     6061 acgacggcaa cgtgaaggtg gtcgacttcg gcatcgcaaa ggcggcggcg aagaccgagg
     6121 agacgcaggc cggcacgctc aaaggcaaag cgtcgtacat gtcgcccgag caatgcacgg
     6181 gcagcaaggt cgatcgccgc agcgacgtgt tcgcgctcgg catcgtgctc tacgaggtcg
     6241 tgacaacgcg ccgcttgttc aaagcggaga acgacttctt gacgatgagc gcgatcgtcg
     6301 ccggcaagat tccgtcggcg aagcagtacc gccctgacct gccggatgcg ctcgaggcga
     6361 tcctcctcaa ggcgctcgcg ctcaaggtcg aggaccgcta ccagaccgcg gaccagttgc
     6421 gcgaggcgct cgagcagttc gcagcaaacg ccgggcttcg cacgtcgacg agcgcgctcg
     6481 cggcgtacat gaagcaacaa ttcggcgagc gccccgagcc atggctcgtc gatgacgacg
     6541 aaccggagat ggagctcgtc gtcgacttcg atggttcgca gagcgcgctc gtgtcgccgc
     6601 cgcaggagtc gctgcacgag ctcgcgattc cgacggacgt cgaagcgctc aacagctcgc
     6661 cgatcgtgaa ggcgcgcacg aaggagattt cgaagacgcc cgcgacgacg ccagcgccgc
     6721 cgaagcgcca gatgccactc gcgccgccgg gaccactacc aaagcaagcg aagctgccga
     6781 ccgcgcagat tccgacgctc ggagtgccgc cggcctcgga caagtcgcga ccgttccgca
     6841 tcgacaccgg tccggtgccc gcgccagtgc cgcgcaagaa accgatcacg gtgcagattc
     6901 cggtggtgaa ccaagccgcc ggtgtggcgc ccaagcgcgt cgagtctccc ggcggtctac
     6961 ccggcgtcgc gacgaagaag tcagacggcg ttccgcaagc cgctgacgcg agcgattcgg
     7021 ccgcaacgtt cgtgagcggc gcggtggaca atgtcgatgg cgcgtgggac gtgagccagg
     7081 cgttcgtcgc cgcgacgtcg aaggcggccg gcgtcaacgc gttgcctgtc gacggcaagg
     7141 cgagcgatcc gaagggtgag gcgaagcctg ctctcgacac caaagccgcg accaagccgg
     7201 ccgatgcaaa gcgcgagagc aaaccagccg acaccaaggc gagcgacgcc aaggtcgaga
     7261 acaagccgaa ggcgaagcca gcagccggta cgccaccacc ggccatgccg ccgcgcaaga
     7321 cgccacccat cccgttcgcg aacgtgggcg gcgcgcagcc cgtgccgagc gtcaagagca
     7381 agccgagcga cgccgtgccg ggtgcagcca agcccacgga cacgcagccg acgagggtcg
     7441 cgcccaagcc gggtgactcg cagccgatcg aatcgaccgc gatcgattct cgatctgcgc
     7501 cgcccctcgc ggccaagccc accgacacgc agccgacgag ggttgcgccc aagccgagcg
     7561 actcgcagcc gctcgattcg acggcgatcg attcgcgccc tgcgctgcca ctcgcgcccg
     7621 ctgactcgca gccgatcgac gcgacggcga tcgattcgcg gcctgctggc gcgcggcccg
     7681 tcacgccgtc gccggtagca cctgcgttcc agacgggcgg aacgccgaca ccgacgccgc
     7741 cgacgccgac gccgacgccg acgccgctgc cgcccgtctt cgcgccgacc gacaccacgc
     7801 cgccgccgcc cgtgttcgcc cacgcgacaa ccccgctgcc gccgaagttg ccgcccgcgc
     7861 cggagcccaa gacgtggtcg ctcgccgagc tagcgtcgat gaagggcgag aagcccgtcg
     7921 acaagaacga cgccagcacg acctcgaagt tcgacgtcgg cagcctgtcg acgtcgcgca
     7981 ccgacgcgag caacgtgccg gtgccgtatg cgccgaccgc gcatgcagca tcgccggccg
     8041 acgcgatcgc cgctgccgat gccgccgccg acaaggtgaa gcgccccggc accgacggac
     8101 gtgacagcgc gccttcgctg ccgccgccgc ccgctgcgcg cgtgaagctg ccgggcgtcg
     8161 cgcaaaccgg cgaggccgcg ccggggaccc tcgcctccaa gttcgcagcg ccgaatgtgt
     8221 tcacgccggg tcgcacgaag acgccgtcca cggtgcccga tacatgggct ggcggcacat
     8281 cgacgggcaa ggctctgtct ccgctgcggc gcctcctgcg atcgccgatc ggtctcgcgc
     8341 tcgtcgccgg cgcgctcgtc acggtggtcg tcgtgatcgt gttcgttacc cgtggctcga
     8401 agccgactcc tgcgaaggca actccgccga gcgagcctgc gcacgtgacc ctacctgccg
     8461 agacgcagcc ggccgagacg caaccggccg agacgcaacc gaacgaaacg cagccgaccg
     8521 aaacgcagac gccgcgggtc gatcaagccg cgacgaaacc ggtgacgaaa ccggccacga
     8581 agcccacgac caagcctgcg acgaaccaga tcacgaagcc ggtcgcgacg aagccaacga
     8641 ccgtgacgaa gccaacgacc gtgacgaagc cggcgacgac gaagccggcg acgacgaagc
     8701 cgacaacgac gaagccgaac acgcagatca ccaaaccggc gacgacgacc aaaccgacga
     8761 caacgaaacc ggcgacgaag ccggcgacca aaccggcgac gaaaccgacg aagcctcctg
     8821 ccaagaaatg ggatccgaat accctcttcc cgtcgcgaaa gtagagccat gaagcattcg
     8881 cttatcgttg cgctgatcgc gctgcccctc gttgcgttcg cgggcccgaa ggaaaagaag
     8941 gaagcccagc aacacatcga gaaagcgacc tcgctacaca aggcaggcaa gtacgccgaa
     9001 gcgctcaccg agctgcaggc cgcgtacgcg ctcgatgcgc agcccgatct gctctacgcg
     9061 atcggtcaga tctacgtgaa gctcgacaag tgcgacgagg cgatctcgtt ctacgaaaag
     9121 ttcatcgcga cccagccgcc cgccgacgcg gggaacagcg cgaacgaagc gatcgaaacc
     9181 tgcaaggcca agcagccgcc accgccgccg ccgcccgagc ccacgccgcc accgcagccg
     9241 ccgccaccgc caccacaacc cgagtcgaag ccgttctaca cggacaagat cggcaccgcg
     9301 ctcgtcggcg gtggcgtggt tgccatcgtg gtcggcgggc tgttgtactc gagtgcgcgc
     9361 agcacgctcg acgacgccga tgctgcgatg acctacgccg cccatcagga gctcgtcgat
     9421 gacgcgcacc tgaaacgcaa catcagcgtc ggggtcggca tcgtcggcct tgccgcgatc
     9481 ggcgtcggcg tctggcacta catgcaatat cagtccgaac aatccgttgc cgtgaccccc
     9541 acgaccagcg gcggcatggt tacctggatg ggacggtttt gatgcgcgcg gtcttctctc
     9601 tcttcgctct tgtcgcattg gccggcagca gcgctggctg cctgcgcacc accgagttca
     9661 agtgcacgag cgacgcagat tgcagtgcga gcggcgcggt gtgcgagagc acgagctact
     9721 gcagcttcac cgataccgac tgcgcgatgg gccgtcgcta tggcgagctc tcgggcccgt
     9781 actcgaatca gtgtgtcggc gagacgtcga tgaacgacgg cggtatcgac ggtcctgatg
     9841 gcggcaccgg gaactgcccg gccacgtacg ccgccctgcc cggtgcgggc ggccacgtct
     9901 ataagctcac gaataactcg cagcagtggt cgacccagcg cgaccgctgc gccaatgagg
     9961 gcgcctacct ggcgattccc gacgacgcga acgagctgat ggcgatcacg accgccgcgg
    10021 ccgccgccaa aacgtggatc ggcatcagcg ataccgcgat ggagatgacg tacgtgacgg
    10081 tgaagatgac cccagcgacg ttcctgccct gggcgagcgg tgaacccg
//
LOCUS       DASUFX010000689         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1915617, whole genome shotgun sequence.
ACCESSION   DASUFX010000689 DASUFX010000000
VERSION     DASUFX010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915617"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..2458)
                     /locus_tag="VFV99_27790"
     CDS             complement(182..2458)
                     /locus_tag="VFV99_27790"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX5063222.1"
                     /translation="MGRKEDALDYHSRGRKGKLEVVPTKPLVSQIDLSNAYTPGVAEP
                     CLEIAADEEKSWEYTARGNLVAVISNGTAVLGLGNIGAAAGKPVMEGKACLFKKFADI
                     DVFDLEVAEKDVDKFCDLVAALEPTFGGINLEDIAAPACFEIEEKLRKRMRIPVFHDD
                     QHGTAIISGAGLINAAQLAGKKLEEIKLVVSGAGAAAIACVEFFCSLGLKRENAVLVD
                     SKGVVHKDRTDLNRQKAKFAIPDDGRRTLADAMLGADMFMGVSVAGAVKPEYLRGMAE
                     RPIIFALANPDPEIPYPDAIAARPDALVATGRSDFPNQVNNVLGFPYIFRGALDCRAT
                     TISEEMKLAAARALAELTREPVPDTVVMAYGGKTLKMGPDYFIPKPFDPRVLWWVAPA
                     VARAAMDSGVARLKFDIGEYREKLMSKSSNAAYSIMRTIGREARRDPKRIAFPHAANP
                     RVLRAVQQIAEEGIAKPVLLGRPAEIEQMCNEMSLDMMSRGVEIIDPRTEKTQSYVDR
                     LWQLRQRKGITRDAAYALVQKSDYYAALMVDRGDADGIVTGLRLNYPETVRPLLEIFG
                     LAPGSKVAVGMYMMVLKNSVKFFADAVFNIDPDADTLADITLQTADAVQSFGVTPRIA
                     MISYSNFGAVEHPGTRKIKQAIDIVRRRRPDLEIDGEMQPEIALDNERRNNYYPFSRL
                     SRSANTLVFPYLDPANAAYQTLKVLGNASAVGPILLGLSKPCAALPNEVTVEDIVNMT
                     AYVVLNAQQNRAREALRA"
     gene            2501..>2875
                     /locus_tag="VFV99_27795"
     CDS             2501..>2875
                     /locus_tag="VFV99_27795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063223.1"
                     /translation="MGWLSDLLRGGDGELGWDDLIRRTVEEVAKLRTYGQRGETVFPE
                     DVIVRITVPEGSTAVLQGFIDKPELDREVGAALANKCDVAVDALPSREYIVSVADRAS
                     VTASEGLAKSWRLVIAGGDLDGQ"
BASE COUNT          483 a          898 c          967 g          527 t
ORIGIN      
        1 tgatagaagt tcgagatgaa cgtgccggtg tagaccccga cctcgatcgg cagcggctcc
       61 tcgctcgccg gcgtcggttc ggcgctggtt aatgccgcct ctcctgcgtc tgcatgcgca
      121 gacgtgaacg accccaggac gagaagaaca gcgacgtggg tgctacgcaa tcacgaatat
      181 tttacgcccg cagcgcttct cttgcgcggt tttgctgcgc gttgaggacc acataagcgg
      241 tcatgttcac gatgtcctcg accgtgactt cgttcggcag ggcggcgcag ggcttcgata
      301 agccgagcag gatcggtccg accgccgacg cattgccgag caccttgagc gtctggtagg
      361 cggcgttcgc cggatcgagg tacgggaaca cgagggtgtt cgcggagcgc gacaggcgcg
      421 agaacgggta gtagttgttg cgccgctcgt tgtcgagcgc gatctccggc tgcatctcgc
      481 cgtcgatctc gaggtccgga cgccggcgcc gcacgatgtc gatcgcctgc ttgatcttgc
      541 gcgtaccggg gtgctcgacc gcgccgaagt tcgagtacga gatcatggcg atgcgcggcg
      601 tcacgccaaa gctctgcacc gcatcggcgg tctgcagcgt gatgtcggcg agtgtgtccg
      661 cgtccggatc gatgttgaac accgcgtccg cgaagaactt cacgctgttc ttcaacacca
      721 tcatgtacat gccaactgcg accttgctgc cgggtgcgag accaaagatc tcgagcaacg
      781 gccgcacggt ctcgggatag ttgaggcgca ggccggtgac gatgccgtcc gcatcgccgc
      841 gatcgaccat cagcgcggcg tagtagtcgc tcttctggac gagcgcgtac gcagcgtccc
      901 gggtgatgcc cttgcgctgg cgcagctgcc acagccgatc gacgtacgac tgcgtcttct
      961 cggtgcgcgg atcgatgatc tcgacgccgc gcgacatcat gtcgagcgac atctcgttgc
     1021 acatctgctc gatctcggcg ggccggccga gcagcaccgg ctttgcgatg ccctcctcgg
     1081 cgatctgctg cactgcgcgg agcacgcgcg ggttcgcagc gtgcggaaac gcgatccgct
     1141 tcgggtcgcg acgcgcttcg cggccgatcg tgcgcatgat cgagtacgcc gcgttgctgc
     1201 tcttcgacat cagcttctcg cggtactcgc cgatgtcgaa cttgaggcgg gcgacacccg
     1261 aatccatcgc tgcgcgggcg accgcgggcg cgacccacca cagcacgcgc ggatcgaacg
     1321 gcttcggaat gaagtagtcg gggcccatct tcagcgtctt gccgccgtag gccatgacga
     1381 cggtgtcggg cactggctcg cgcgtcagct cggcgagggc gcgggcagcg gcgagcttca
     1441 tctcctcgga gatcgtcgtc gcgcgacagt cgagcgcgcc gcggaagatg tacgggaagc
     1501 cgagcacgtt gttgacctgg ttcggaaaat ccgagcggcc ggtcgcgacg agtgcgtcgg
     1561 ggcgcgcggc gatcgcgtcg ggatacggaa tttccggatc gggattcgcg agcgcgaaga
     1621 tgatcgggcg ctcggccatg ccgcgtaggt actcgggctt cacggcgccg gcgaccgaca
     1681 cgcccatgaa catgtcggcg ccgagcatcg cgtcggcgag cgtgcggcgg ccgtcgtcgg
     1741 gaatcgcgaa cttcgccttc tgacgattga gatcggtgcg gtccttgtgg acgacgccct
     1801 tgctgtcgac gagcacggcg ttctcacgct tgagaccgag gctgcagaag aactcgacgc
     1861 acgcgatcgc ggcggcgccg gcgcccgaaa caacgagctt gatctcctcg agcttcttac
     1921 cggcaagctg cgcggcgttg atcaggccgg cacccgagat gatcgcggtg ccgtgctgat
     1981 cgtcgtggaa caccgggatc cgcatgcgct tacgcagctt ctcctcgatc tcgaagcacg
     2041 cgggcgcggc gatatcctcc aggttgatgc caccgaacgt tggctcgagg gccgcgacga
     2101 ggtcacagaa cttgtcgacg tccttctcgg cgacttcgag atcgaacacg tcgatatcgg
     2161 caaacttctt gaagaggcag gccttgccct ccatcacggg cttgccggcg gcggccccga
     2221 tgttgcccaa gcccagcacg gcggtgccgt tcgagatcac ggcgaccagg ttgccgcgcg
     2281 cggtgtactc ccagctcttc tcttcgtcgg ctgcgatctc gaggcacggc tcggcaacgc
     2341 cgggcgtgta cgcgttggac agatcgatct ggctaaccaa cggcttggtc gggacaactt
     2401 cgagctttcc tttgcgccct cgcgagtgat agtcgagtgc gtcttccttg cggcccattt
     2461 gggggaacgt agcaaaaatc caagctacga tatggctcgc atgggctggc tctcggacct
     2521 gctgcgcgga ggcgacggcg agcttggctg ggacgatctg attcgacgga ccgtcgaaga
     2581 ggtcgcaaaa ctccgtacct acgggcaacg cggcgagacc gtgttcccgg aggacgtgat
     2641 cgtacggatc accgtccctg aaggcagtac agcggtgtta cagggattca tcgacaagcc
     2701 ggagctcgat cgggaggtcg gggctgcgct cgcgaacaag tgcgatgtcg cggtcgatgc
     2761 gctgccgagc cgcgagtaca tcgtgtccgt cgccgatcgc gcttcggtca cggcaagcga
     2821 gggcctcgcg aagagctggc gactcgtgat cgccggcggt gacctcgatg gccag
//
LOCUS       DASUFX010000690        19802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_944866,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000690 DASUFX010000000
VERSION     DASUFX010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944866"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..736)
                     /locus_tag="VFV99_27800"
     CDS             complement(137..736)
                     /locus_tag="VFV99_27800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063224.1"
                     /translation="MLVLLLVAGLSSTARADDDEPPITFHKGQFGLSARLGLGVRGIA
                     TYENTVYCGKLDAQAENGFASVCTGRLPIALDLEGSYGVAQSIELTLDLRVGIESDFG
                     SNQSTDGPHPLQLSPGARFFFSEAKHTKLFVQPGIVFDFADYSQRPGNDFGVRGIEGF
                     WIDLHRSYGFYFWIAETVEFSRWLSFAFEGGIGFQGRYP"
     gene            781..1575
                     /locus_tag="VFV99_27805"
     CDS             781..1575
                     /locus_tag="VFV99_27805"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5063225.1"
                     /translation="MLTRRQLLGGAAAGLALALSRRAHAIGPGSKFRIGHLQLGPGQS
                     WNPRPSALKKLGWEIEKRTSIDVDDPQIVTPTSDTLHETPFLYLTGEKAIEVPSNAGV
                     DALRRFCTFGGFLLIDSAEGSTDGAYAGSVRKLISAIFPPPAKGLEIIPADHVVYKSF
                     YLLDKPVGRLAIAPAMEGVIRDGRLVCAYIPNDLGGAWARDDFGNYDFPCEPGGEKQR
                     ELSFRLGVNLVMYALCLDYKSDQVHVPFIMKRRRWKPDDGASSPTK"
     gene            1572..4067
                     /locus_tag="VFV99_27810"
     CDS             1572..4067
                     /locus_tag="VFV99_27810"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5063226.1"
                     /translation="MNIVLFSMSWRIAIAIATVVLALAALIAAKQGRGRASTVLGVGA
                     LAGLGALAGEGLVALTVRIAAPAATDFNEYRWVFLSPYGRWGLYVGAIVVGGIVALSW
                     RSSRGASAWRRAILIGLRGGAAIVAMVVFLEPAVELRQVAREPNRIAVLIDDSKSMSL
                     AENPAGPTRIERVRRLIANSQSTLASWEREHKVDYYTFAETVSATSPAALAADKAQGH
                     ATLIRKALEFVRGRYEGRDLAGIVLISDGSATGSFDEDSGDGAVRDFLRSLDTRVHTV
                     WAAREGLRDVAVARVMADEFAFVRTVVRIEAVIRTTGLGARRVPVTLSTDGQPLREKQ
                     IDLPAGDHEVTVSFEVTPPRVGRYVYEISVPVAPGEAVTTNNTRSFVVRVIRDKIRVL
                     QVAGSPSWDVRALRAMLKSNPNVDLISFFILRTQDDISLVPNDEMSLIPFPTRELFEQ
                     ELPSFDVIVLQNFEYLPYGIGDYLENIRSYVEGGGGLAMLGGAQSFTSGGYYGTPVAA
                     ALPVDLYGPFDSGPILDTQKFSPVLTTAGQQHPVTSLRYSANDNLAAWKDLPPLEGVN
                     LVAGAKADATVLATHPKLKTKGGKPMPVIVAGDYGKGRSLVFTTDTLWKYGFVAASRP
                     GDNGRQYTKLWENSIRWLIQDPDLRNLHVDSDAVEYVPNAPVRVTVRLLGRDYQPLPK
                     GEVALVVKRGANPATAEQVQQTKLTVGDDGTAVYELGGLTPGVYRVQGSAKIAGRDVD
                     ASDIFLVREGGTELDRPVGDRDMLEDIAAATGGSALGPVDELPSHLKMDPPRIVRVDK
                     RTDIELWSRPGLLFLIVGLLGLEWLLRQRSGYL"
     gene            4082..5293
                     /locus_tag="VFV99_27815"
     CDS             4082..5293
                     /locus_tag="VFV99_27815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063227.1"
                     /translation="MLQLDSIVTDLMFQSTAAIDQQTLIDGLGGEDVLQTKQLLDVDR
                     LDPRAEMELSPSMPFRRRGERRLMIIDDQATTPRTDPQNDPLIKVDLRPYKESLIASC
                     QTLPVRLGRIFALGSWGDAVIVARSVRDLRGICLLPYALDPQVDIDGKRRQTPLSQKE
                     FEAKLMAYEKRLEELDDQQILARLSGVSFERRGDVVVVDVLEADGTWDQRKSMLLEQQ
                     LAAVDTFSMIPGAPSSTAPKAAAPAKSNGEPAKAKPAAAAPKPEPKPEPVKPKGPPLA
                     AKEIDGKVVLVFPAERFDLDVAAALGKRDWDTVVHSSDNLSGAVRDKIHREGASFVAP
                     LEFLSEVFVEGKPLTKQQFEKDARAVDGARALDVHFPRFGEVVLLELPGKGRFVTSLT
                     DRAERVVALVK"
     gene            5311..6450
                     /locus_tag="VFV99_27820"
     CDS             5311..6450
                     /locus_tag="VFV99_27820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063228.1"
                     /translation="MSLVAALATGVGASAADDEFAGTAIVFVRGSALIKSDGKGRGET
                     EIATLADKAQVRALRTDAVGKILLADIGGTWSWMPLDGSTKALTQLPCEAGPAQLSED
                     GRYVFCRKGTGSLVVNLVTGKLTSLPVPTVGARLLGSPGVGGNTRPLVWADKGAIWSA
                     IAPKLDKPRKVVPEAPLRNLMVAPDGTHAIGTYQGDVYESPRGAKKPAEVLMTFALDG
                     QGARRKAIQNGVPVDWSFDSKWVLVQDGASACIMLVAGGQYKCWRGFTAVSISPDGRY
                     ALVLGNRSSDSDKKADKKADKKSKKKSDKKKKDTDQDAIGEAAEGDTGDEPVPTDDVA
                     VPPPSGPRSLYRAQLEGAYTTSPALVARVVEGAAVWIPTPTTAAP"
     gene            complement(6447..7004)
                     /locus_tag="VFV99_27825"
     CDS             complement(6447..7004)
                     /locus_tag="VFV99_27825"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX5063229.1"
                     /translation="MAACGHGGWAPGNYEKYPPPPHEVVLGVGDIIDINVWEQKDLST
                     EVTVRPDGNITIPLVGDVQARGKTPTQLRDDIKKSLGNYLKLSAGNEVVVIVKTYNSY
                     AFTVNGEVSRPGLYMNPKYLRVADAIALAGGPTRFAKRTDVRVLRTDIKGNQLSIPID
                     FDLVASGKRPDMNIWVLAEDVIYVP"
     gene            complement(7090..8181)
                     /locus_tag="VFV99_27830"
     CDS             complement(7090..8181)
                     /locus_tag="VFV99_27830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5063230.1"
                     /translation="MTIAVLFDQLIQAGASDLHLSPGYPVMFRLRGKLVSASERTLTP
                     EQIEALLMPMLTDIQRTQFVTTGDLDFAHQHDRRARFRANFMRKTTGMGAVFRAIPTK
                     ILTLDDLGVPPGVRKLAELRNGLVLVTGPTGSGKSTTLAAMLDHINRTRSGHILTIED
                     PVEFVHEPRSCMITHKEVGEHVPSFVEAIRSAGRENADVILVGELRGAETMKAALQLA
                     SFGILIFATVHTNSASSTIDRFVNAFPAEQQPQIRGLLADSLAGIVAQQLLPRADKGG
                     RIAAHEILMGNLALSSLIRESKTQQIPSLIQQGLSEGMQAMDSALEKLVSAGIVSAQD
                     ALDKALDKDAFARIPIVARQLAPEQRADL"
     gene            complement(8178..9803)
                     /locus_tag="VFV99_27835"
     CDS             complement(8178..9803)
                     /locus_tag="VFV99_27835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5063231.1"
                     /translation="MSAKLDQLLGYLDRADVTEIIVGIGRPIALKTGQGYMQLTSLPV
                     SELQFATLVGGTALDKIHKTAGAQPIDCDVGRRRVRVELVRRPEGSALRIQAAPAAAP
                     APAVEPVAVPMVKFATPQAAVAEPADDDEGVELGAPVHDSEPVQATAPAQPEAQPDPV
                     ETAEDLGEDAIFDEQIDSRGIERYVRAARQKGASDLHIVAGRPAMVRLMGELRPLDPT
                     PVPADIVDRMLLPLLGPRRRVRLRTVGYVDLAFDVQGAGRLRGNVSRLQGGLRGAFRL
                     ARVTPPTLEELDLPRELAKVTAHHQGLVVIAGPNGQGKTTTMTALVDLINSTKAHHII
                     TIEDPVELEHPRKQSVISQREVGRHTKSFAAALKASLREDPDVIVIGELRDRETVEIA
                     LTAAETGHLVLATMSTPSAAKTIDRLIDMFPPDEQTQARASIAGALKAIVAQRLLPST
                     AGGVVPAIELVTGVLPLAVLIRDDKLFQLPNLMQRGRAFGMIRFDDSLLELLRAGKIT
                     EDTLRQYSPTAANQAKQPAQPAAQPMGRGGRRE"
     gene            complement(9806..10339)
                     /locus_tag="VFV99_27840"
     CDS             complement(9806..10339)
                     /locus_tag="VFV99_27840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063232.1"
                     /translation="MKRSSERGSAMLVAMILMAALLAGAAVLVSLQLGSNRNTELTKT
                     GISALYCAEAGLTATRQTVAQNSDTWGTYLGLPKTLSTSEPAWLANISHDVDGDTSND
                     PDWRVTLEDNNDEIPASGENPNADVDSRIYIVSQCLRYPDMPREVRELITFTTVTHCY
                     SAQKGGCGGNGNMNSAP"
     gene            complement(10336..11325)
                     /locus_tag="VFV99_27845"
     CDS             complement(10336..11325)
                     /locus_tag="VFV99_27845"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX5063233.1"
                     /translation="MNREAQQIARAQRGFTLVELMVSLVIFSFAIAGVLSVAVAMTRA
                     HREQRRVVQTENAVRAPLDFIVDAIRQASPGVTTGTIKDAHDCTFSSGAISFVDSEIG
                     PDELTVVYASGGIVSTTHSAFTSTSTSITIPAAHLSQFAIGDYVLVTDTTQGTLARVS
                     GVTSTALSIDTITCTTAFPAGGYPQGSILLRAQRARFTVGAVDGIPTLLMYPNGSSSY
                     EPLAEGIEDMQIAVGVDSNSDGALTPASPFLTGGTTSDEWAGNVATDTLPAGTIRAVT
                     VVLVSRDTAPLTGTASFYQPGDALNHPGSTTADRYRRRMLSTTVEIRNLAGSP"
     gene            complement(11322..11726)
                     /locus_tag="VFV99_27850"
     CDS             complement(11322..11726)
                     /locus_tag="VFV99_27850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5063234.1"
                     /translation="MAPDRVTRRSEQSGFTLIEVMVAMLLTALTVIGVLGLYRVESRA
                     SSFSRRETEAAVLAQDKLEELRTQAAPTAASGPTQDPAIVDQLTGATIFTRTYTIATT
                     SDPLVFKIIVTVQWDEDGVPRTVTVTGQRGST"
     gene            complement(11708..15559)
                     /locus_tag="VFV99_27855"
     CDS             complement(11708..15559)
                     /locus_tag="VFV99_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063235.1"
                     /translation="MLGLVVRSHEARADFAQCSAPLSACTVTSSGCCTASFNMTSGTH
                     HPLIIAMDRCHQPIGSGTSGPPGVSPPRKEWSISSISRTGSTVTVVTTANHGAGNGDY
                     VKIVGTGNSALNGTFVVTNGCSNCTTLTYSTTSSGNISAITTGKALTFNMGNGWCGDA
                     PGSATRIGGNAADDGMFKTYGLVYRLMQRAIPVYWLVNPGKAIVALNSGQNAGGQTYL
                     ANDVDLWVLTGDLTDPPTSADPLIACGSGCTQPVHRLTSATLVPKTDSYQYKQFPVRG
                     GTFLIAAEDRANFDDFWLRQGAYSSLDATKYNWSNSGIDLYELDPSAKLVYQDYTSGD
                     GTSSNRFTTVNGAPLAVKIDYEPPRIACVGCNNNVAQGWLGTAGIQDPASPASCASGE
                     FSPSDATYCVLNDTDVAQGTLVSGGFGWLWLFGYNDNSPCGNSAEKAVFDKVRDFMTS
                     LPAIRNAGHGVFLDDSIKVAEGCPNKQLLGKPSSGDGLDIQNNGNTEPFIMRYPSNLF
                     MQSGDLVPASANGTVQGWKYYKNGSPNVGYQTPFQVSTSSLRRLMTADTTGTNNTACV
                     LHESTTDCDSFTPSAATGDLLDVATYARFKDQLNNGLAYYLAGNQLNNSGSYAELRML
                     LNSLLALPDETFTSSPTIVEVSRSGPIVATDEDSTVTVFQGTYEHFDPIPNIPKATTS
                     AGLARFTFPYALGHLRQIPVASYAACSAAGCDTGNGSRTAISSLSGVVFDAADHIPTV
                     NTAGCATNFNGSCRTVFTTLQSGRLPPHVMFTTANYNATTASAPTDLGNTLASNFTTA
                     EKQTFISRILAGRKDASNNWIPKLGGIDRSTVAVIEPSTIAGSAGRPTMAYVGATDGM
                     MHAFCVTTGGGCDEVGRELWAYIPRTVLPDLRQSTAHIDGSPNVIDAYGDFDNNGSNA
                     WHTILIFHTGTGRMTANNVVPAVYAIDVTAPNNPRVLWEYTVTAVGSRSTYELGVGLN
                     LTAGTVTISGVKKTVAYVQTQNGGTGGAASVLTAIDVETGAKIWQKGDLYPSPRAGTH
                     EGAPSAGIPGGAVGLDTTGTGRINKVVYGTLYGDVFVRDAATGANQNGTVSGVDTPLM
                     RLATDYHPIGAPPAILAKNGTQYAAFATGGYADSQLTLWRGENESPVPTQMVFAVTTS
                     YSGTSSLTDAETDLTKLPIKFSLGAGEAGFSQLTVVGNQLFVTTDTTNINDYAFGTSS
                     TPTGHAYNYDFGAATPSLGTTLIIASGAASLFNNGTTMITGSGRYGEKLTTDALGTSG
                     AAVDPMATSNQLRRALWLRTE"
     gene            complement(15579..16127)
                     /locus_tag="VFV99_27860"
     CDS             complement(15579..16127)
                     /locus_tag="VFV99_27860"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5063236.1"
                     /translation="MQSPTRSTQRGFTLIELMVVVAIIAVLAGLMFGVAPRPYDANAE
                     VIASQINSTFIYAHTRAVSTRKVHRVVFDTDTSTDEQYVEVDVAPSTGMSYSGTGWLL
                     LEVKRVPKNTIIWSAEDSAVTSTGATPAQDTNLPGYYVDFKPDGSATASTIYITDRNS
                     TVKQYRVLVYHVTGSSYARESW"
     gene            complement(16127..16648)
                     /locus_tag="VFV99_27865"
     CDS             complement(16127..16648)
                     /locus_tag="VFV99_27865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5063237.1"
                     /translation="MHQRATQRGFTILELMIAVAIIAILAVVVIPSFIKESKRGKSRS
                     EVDPMFAELSTREEQYKVENSAYLAMAACPPTASSTGTDMTTPTCVTTATLPWTLLRV
                     QAPQSKLTCSYVIARGNAGDQPTADGAWPGTWAGAPPSAPALSWYFITADCPDTDYIF
                     KSWDTKIKAADGH"
     gene            complement(16707..18365)
                     /locus_tag="VFV99_27870"
     CDS             complement(16707..18365)
                     /locus_tag="VFV99_27870"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5063238.1"
                     /translation="MKRALVPAFLVLAACGSTPKTTTPAPVPPSPTPTPAVTPKPPEN
                     TMPAVSQTAQPQELAFPDEDFRRTQPAPSASRDFKLPPVKPFTLKNGIKVYLVEQHTL
                     PIVSMDLNFDGGSMTDPNGKDGLAGLCMSLMTEGTQKLDKIQYSEALADTASNISTYA
                     SDDSQGIQLASLTKHVDATFALFADTILAPGMRDSDFDRLKKRRIESVRQSRTNPTSI
                     PFRVSGPVLYGADHPLGAVITEGGLESITLDDCKKQVSTWMKPKNARLFVVGDLTEAQ
                     VRETFEKSPLGSWTGSAPKVAALPAAKTMKGKIFFVHVPNAAQASVSLMWFGPKRNAP
                     DYFANSMLGAVLGGGFTSRINMNLREDKGYSYGARGGFSYPLKGLGTVNVTAGVQADS
                     AYQTLLEIDREVKELAAGKTPVTAEELKREKENAELAMPARFSTAQSALGQYRSLVYY
                     GLPLDYYNSYVAKVRKVTEKEVKASATKYLQTKGAVYVVVGNGDEKMIVDKGPKGAPP
                     KDRKAPYEKDGKQLTLREALAELAKTSVGAGGLVELDVDGKVVK"
     gene            complement(18362..19693)
                     /locus_tag="VFV99_27875"
     CDS             complement(18362..19693)
                     /locus_tag="VFV99_27875"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5063239.1"
                     /translation="MRPLLVVAAVLAVSAPAFADDPKLEFEKYKLPNGLEVILAPDNS
                     VPLVAVNVWYHVGSGNEVVGKSGFAHLFEHMMFQGSKNVGSDKHFEILKKIGSSNVNG
                     TTNTDRTNYFEVIPSNQLEVGLWLESDRMGHLLELVDRAALDNQIDVVRNERRQNYDN
                     VAYRKALFALYAAMYPEGHPYRYLTIGKHEDLTAASLDDVKNFFKTWYVPSNATLAIV
                     GDFDVAATKKLVEKWFGGFPTSKKPTWVNVPAPAQKAATKNVDDSFAKQRQVTWAWQS
                     PANFGDGDAELDIVANALTAEGRGRLYKSLVYDKQLATSVRAGQNSMQFSGQFSVTVT
                     LRTGADLEAVQKIVFDELERVTKENLSDKEISRFVAATEAAVIYRLEDVNSRANTLQS
                     YNHFLGDPGKLTWDLDRYRKTTPDKIRAAAAKYIVPANAVTIITNPTGGAK"
BASE COUNT         3249 a         6381 c         6620 g         3552 t
ORIGIN      
        1 ccgttgccgc agccggcatc ccacgcgagg tcgcggtgcg cgcagcgttc ggcgagcgcc
       61 acagcgagcg ccttcgggta ccgcgggcga taggtcgcgt actgagacgc gatggtcgag
      121 aaatgatcag cgaagctcag gggtaccggc cttggaaacc aatgccaccc tcgaaggcga
      181 aggacagcca acgcgagaac tcgacggttt cagcgatcca gaagtagaag ccgtacgagc
      241 ggtggaggtc gatccagaac ccttcgatgc cgcgcacgcc gaagtcgttg ccaggacgct
      301 gcgagtaatc ggcgaagtcg aacacgatgc cgggctggac gaacaacttc gtgtgttttg
      361 cttcgctgaa gaagaagcga gcgccgggcg agagctggag cgggtgggga ccgtcggtgc
      421 tctggttact cccgaagtcg ctctcgatgc cgacgcgcaa atcgagcgtc agctcgatcg
      481 actgcgccac gccgtacgaa ccctcgagat cgagggcgat cggcaacctg cccgtacaaa
      541 ccgacgcgaa cccgttttct gcctgggcgt cgagcttgcc gcagtagacg gtgttctcgt
      601 aggtcgcgat gccgcggacc ccgagcccca ggcgggccga gaggccaaac tggcccttgt
      661 ggaacgtgat cggcggctcg tcgtcgtcgg cccgggcggt cgacgacaaa ccggctacca
      721 gtagcagcac cagcatcccc cgcattgcag cgacgctatc acgaggctag ggttaccggg
      781 atgctcaccc ggaggcagct actcggtggt gccgccgcag gtttggcctt agcgctgagc
      841 cggcgagcgc acgcgatcgg cccgggttcg aagttccgga tcggccacct ccagctcggg
      901 cccgggcagt cgtggaaccc gcggccgagc gccctcaaga agctgggctg ggagatcgag
      961 aagcgcacgt cgatcgacgt cgacgatcca cagatcgtca cgccgacgag cgacacgctg
     1021 cacgagacac cgtttctcta tctgaccggc gagaaggcga tcgaggtgcc gagcaacgcc
     1081 ggcgtcgatg cgctgcgccg cttctgcacg ttcggcggct tcctgctcat cgacagcgcc
     1141 gagggctcga ccgacggcgc gtacgccggc tcggtgcgca agctgatctc ggcgatcttc
     1201 ccgccgcctg cgaagggcct cgagatcatc cccgccgatc acgtcgtcta caaatcgttc
     1261 tacctgctcg acaagccggt cggccggctc gcgatcgcac ccgcgatgga aggcgtgatc
     1321 cgcgatggtc gcctcgtctg cgcgtacatc ccgaacgacc tcggcggcgc gtgggcacgc
     1381 gacgacttcg gcaactacga cttcccgtgc gagccgggcg gcgagaagca gcgcgagctg
     1441 tcgttccgcc tcggcgtgaa tctcgtgatg tacgcgctct gcctcgacta caagagcgac
     1501 caggttcacg tgccgttcat catgaaacgt cgccggtgga aacccgacga cggcgcgagc
     1561 agcccgacca aatgaacatc gtcttgttct cgatgagctg gcggatcgcg atcgcgatcg
     1621 cgacggtcgt gctcgcgctc gcggcgctga tcgcggcgaa acaagggcgt ggacgggcga
     1681 gcacggtgct cggcgtcggc gcactggcgg ggctcggtgc gctcgcaggc gaaggtctcg
     1741 tcgcgctgac ggtgcgcatc gcagcgcctg ccgcgaccga tttcaacgag taccgctggg
     1801 tgttcttgtc tccgtacggc cggtggggcc tctatgtcgg cgcgatcgtc gtcggtggca
     1861 tcgtcgcgtt gtcgtggcga tcgagccgcg gtgcatcggc gtggcggcgc gcgatcctga
     1921 tcggcctgcg tggcggtgcg gcgatcgtag cgatggtcgt gttcctcgag ccggcggtcg
     1981 agctgcgcca ggtcgcgcgc gagccgaacc ggatcgcggt gctgatcgac gacagcaagt
     2041 cgatgtcgct cgccgagaat cccgccgggc cgacgcgcat cgagcgcgta cgccgcctga
     2101 tcgcgaattc gcaatcgaca ctcgcgtcgt gggagcgcga gcacaaggtc gactactaca
     2161 cgttcgcgga gacggtcagc gcgacgagcc cagccgcgct cgccgccgac aaggcgcagg
     2221 gccacgcgac actgatccgc aaagcgctgg agttcgtgcg cggccgttac gaagggcgcg
     2281 atctcgccgg catcgtgctg atctccgacg gctccgcgac cggcagcttc gacgaggatt
     2341 ccggcgacgg cgcggtacgc gacttcttgc gctcgctcga tacgcgcgtc cacacggtgt
     2401 gggcggcgcg cgagggcctg cgtgatgtcg cggtcgcgcg cgtgatggcc gacgagttcg
     2461 cattcgtccg cacggtcgtg cgcatcgaag cggtgattcg cacgaccggc ctcggcgcgc
     2521 gtcgcgtacc ggtgacgcta tcgacggacg gccagccgct gcgcgagaag cagatcgatc
     2581 tgccggcggg cgatcacgag gtcaccgtct cgttcgaggt cacgccgccg cgcgtcggcc
     2641 gttacgtcta tgagatcagc gtgccggtcg cgccgggcga ggcggtgacg acgaacaaca
     2701 cgcgcagctt cgtcgtccgc gtcatccgcg acaagattcg cgtgctccag gtcgccggct
     2761 caccgtcgtg ggacgtgcgt gcgttgcgcg cgatgctgaa gagcaacccg aacgtcgatt
     2821 tgatcagctt cttcatcctg cgcacgcaag acgatatctc gctcgtgccg aacgacgaga
     2881 tgtcgctgat tccgttcccg acgcgcgagc tattcgagca ggagctgccg tcgtttgacg
     2941 tgatcgtgct ccagaacttc gagtacctgc cctacggaat cggcgactac ctcgagaaca
     3001 tccgcagcta cgtcgaaggt ggcggcggac tcgcgatgct cggcggcgcg cagtcgttca
     3061 cgagcggcgg ctactacggc acgcccgtgg cggcggcgtt gcccgtcgat ctctacggtc
     3121 cgttcgattc gggtccgatc ctcgacacgc aaaagttctc gccggtgctc accacggcag
     3181 gtcagcaaca tccggtgacg tcgctgcgct actccgcgaa cgacaatctc gccgcgtgga
     3241 aggacctgcc gccgctcgag ggcgtcaatc tcgtcgccgg cgcgaaggcc gacgcgaccg
     3301 tgctcgcgac gcatccgaag ctgaagacga agggcggcaa gccgatgccg gtgatcgtcg
     3361 ccggtgatta cggcaagggc cgctcgctcg tgttcacgac cgacacgcta tggaagtacg
     3421 gcttcgtcgc ggcatcgcgg cctggcgata acggccgcca gtacacgaag ctgtgggaga
     3481 actcgatccg ctggctgatc caggatcccg acctgcgcaa cctgcacgtc gatagcgatg
     3541 cggtcgaata cgtacccaac gcccccgtgc gggtgaccgt ccgcttgctc ggtcgcgatt
     3601 atcagccgct gcccaagggc gaggtcgcgc tcgtcgtgaa gcgtggcgcg aatccggcga
     3661 cggccgagca ggtgcagcag acgaagctca cggtcggcga cgacggcacc gcggtctacg
     3721 agctcggcgg cctcacgccc ggcgtttacc gcgtgcaagg cagtgccaag atcgcgggcc
     3781 gcgatgtcga tgcgagcgac attttcctcg tccgcgaagg cggtaccgag ctcgatcgcc
     3841 cggtcggcga ccgcgacatg ctcgaggaca tcgcagcggc gacgggcggc tcggcgctgg
     3901 gcccggtcga cgagctcccg tcacatctga agatggatcc gccgagaatc gtgcgcgtcg
     3961 acaagcgcac cgatatcgag ctgtggagcc gccccggatt gctttttctt atcgtagggt
     4021 tgctcgggct ggagtggctc ttgcgccagc gcagcggcta tctgtagata gaggcaccca
     4081 gatgctccag ctcgacagca tcgtcaccga cctgatgttc caatccacgg cggcgatcga
     4141 tcagcaaacg ctgatcgatg gcctcggggg cgaggacgtt ctccagacga agcagctgct
     4201 cgacgtcgat cgactcgatc cacgcgccga gatggagctc tcgccatcga tgccgttccg
     4261 ccgccgcggc gagcgccggc tgatgatcat cgacgatcaa gcgacgacgc cgcgcacgga
     4321 tccgcaaaac gatccgctga tcaaagtcga tctgcgtcca tataaggagt cgctgatcgc
     4381 gagctgtcag acgctcccgg tccgactcgg ccgcatcttc gcgctcggca gctggggcga
     4441 cgccgtgatc gtcgcgcgca gcgtgcgtga cctccgcggc atctgccttc tgccgtacgc
     4501 gctcgatccg caggtcgaca tcgacggcaa gcgccgccag acgccgctgt cgcagaaaga
     4561 attcgaggcg aagctgatgg cgtacgagaa gcgcctcgaa gagctcgacg atcagcagat
     4621 cctcgcgcga ctctcgggcg tctcgttcga gcggcgcggt gacgtcgttg tcgtcgacgt
     4681 gctcgaagcg gatggcacct gggatcagcg caagtcgatg ctgctcgagc agcagctcgc
     4741 tgccgtcgac acgttctcga tgatccccgg cgcgccatcg tcgacggcgc cgaaggcagc
     4801 ggctccggcg aaatccaacg gcgagcccgc gaaggcaaag cccgcagccg ccgctcccaa
     4861 gcccgagccc aagcccgagc cggtgaagcc gaagggcccg ccgctcgctg ccaaggagat
     4921 cgacggcaag gtcgtgctcg tgttcccagc cgagcgcttc gatctggacg tcgcggctgc
     4981 actcggcaag cgcgactggg acaccgtcgt gcacagctcc gacaacctgt ccggcgcagt
     5041 ccgcgacaag atccaccgcg aaggtgcgtc gttcgtcgcg ccgctcgaat tcctctccga
     5101 ggtgttcgtc gagggcaagc cgctcacgaa gcagcagttc gagaaagatg cacgcgccgt
     5161 cgacggcgca cgcgcactcg acgttcactt cccgcgcttt ggcgaggtcg tgctgctcga
     5221 gctgccgggc aagggccgct tcgtcacctc cctcacggat cgcgcagagc gcgtggtcgc
     5281 actggtgaag tgatgcgtgt ggcgttggcg atgtcgttgg tcgccgcgct ggctacaggt
     5341 gtcggtgcct ctgccgccga cgacgaattc gctggcaccg cgatcgtgtt cgtgcgcggc
     5401 agcgcgctga tcaagtccga cggcaagggc cgcggcgaga ccgagatcgc gacgctggcc
     5461 gacaaagccc aagtccgcgc actgcgcacc gacgcggtcg gcaagatcct gctcgccgac
     5521 atcggcggca cgtggtcgtg gatgccgctc gacggctcga cgaaggcgct gacccagctc
     5581 ccgtgcgagg ccgggcccgc tcagctctcc gaggacggcc gctacgtgtt ctgccgcaag
     5641 ggcaccggct cgctcgtcgt caatctcgtc accggcaagc taacgtcgct cccggtgccg
     5701 acggtcggcg cgcgtctgct cgggtcgcca ggtgtgggcg gcaacacgcg cccgctcgtc
     5761 tgggccgaca agggcgcgat ctggagcgcg atcgcaccga agctcgacaa gccgcggaag
     5821 gtggtccccg aggcgccgct gcgcaatctc atggtggcgc ccgacggcac gcacgcgatc
     5881 ggcacctacc agggcgatgt ctacgagagc ccgcgtggcg cgaagaaacc cgccgaggtg
     5941 ctgatgacgt tcgcgctcga cggccagggc gcgcgtcgca aggcgatcca gaacggcgtg
     6001 cccgtagact ggtcgttcga cagcaagtgg gtgctcgtcc aggacggcgc gagcgcatgc
     6061 atcatgctgg tcgcgggtgg ccagtacaaa tgctggcgcg gtttcaccgc ggtctcgatc
     6121 tcgcccgacg gtcgttacgc gctggtcctc ggcaatcgct cgagtgacag cgacaagaag
     6181 gccgataaga aggccgacaa gaagtcgaag aagaagagcg acaagaagaa gaaggacacc
     6241 gatcaggacg cgatcggcga agccgccgag ggcgacaccg gtgacgagcc ggtcccgacc
     6301 gacgacgtcg cggtgccgcc gccgtccggc ccgcgctcgc tgtaccgcgc gcagctcgag
     6361 ggcgcgtata cgacctcacc agcgctcgtc gcgcgtgttg tcgaaggcgc tgccgtctgg
     6421 attccgacgc cgactacggc cgcgccctaa ggcacgtaga tcacgtcttc ggcgagcacc
     6481 cagatgttca tgtctggacg cttgcccgac gcgacgagat cgaagtcgat cggaatcgac
     6541 agctggttgc ctttgatatc ggtgcgcagc acccgaacgt ccgtgcgctt cgcgaagcgc
     6601 gtcggtccac ccgcgagcgc gatcgcatcg gcgacacgca ggtacttcgg gttcatgtag
     6661 aggccgggcc gcgagacctc gccgttcacc gtgaacgcgt agctgttgta cgtcttcacg
     6721 atcacgacca cctcgttacc ggcactcagc ttgaggtagt tgccgagcga tttcttgatg
     6781 tcatcgcgca gctgggtcgg ggtcttgccc cgggcctgca cgtcaccgac gagcgggatc
     6841 gtgatgttgc cgtcgggacg aaccgtcact tccgtcgaga gatccttctg ctcccagacg
     6901 ttgatgtcga tgatgtcgcc gacgccgagc acgacctcgt gcggtggtgg cggatacttc
     6961 tcgtagttcc cgggcgccca gccgccatgt ccgcacgcgg ccacaatcag taaacacaaa
     7021 gtaaccgaac gcatcgcttc gactcttcag cactgccgcg gaccacgcaa gtgtgcgctg
     7081 gcgcccggtc tacagatcag cgcgctgttc tggcgcgagt tggcgcgcaa caatcgggat
     7141 gcgcgcgaac gcatccttgt cgagcgcttt gtcgagtgcg tcctgagcgc tgacaattcc
     7201 tgcgctcacg agcttttcga gcgcgctgtc cattgcctgc ataccttcgg acaagccttg
     7261 ctggatgagg ctcgggatct gctgggtctt ggattcgcga atcagtgagg acagcgcgag
     7321 gttgcccatc aggatctcgt gcgcggcgat ccggccgcct ttgtccgcgc gcggcagtag
     7381 ttgctgcgcg acgatgccgg cgagcgagtc ggcgagcaga ccgcggatct gcggctgttg
     7441 ctcggcggga aatgcattga cgaaccgatc gatcgtcgac gaggcggagt tcgtatggac
     7501 ggtcgcgaag atcaagatac cgaagctcgc gagctgcagc gcggccttca tggtctcggc
     7561 accacggagc tcgccgacga ggatcacgtc ggcgttctcg cggcccgcgc tccggatcgc
     7621 ctcgacgaag ctcggcacgt gctcgccgac ttccttgtgc gtgatcatgc aggagcgcgg
     7681 ctcgtggacg aactcgaccg gatcctcgat cgtgaggatg tgtccggagc gggtgcggtt
     7741 gatgtgatcg agcatcgcgg cgagcgtcgt cgacttaccg ctgccggtcg gccccgtcac
     7801 gaggacgagc ccgttgcgca gctcggcgag cttgcggacg ccaggcggaa cgccgaggtc
     7861 gtcgagcgtc aggatcttcg tcggaatcgc gcgaaacacc gcgcccattc cggtcgtctt
     7921 gcgcatgaag ttcgcgcgaa accgcgcgcg gcgatcgtgt tggtgagcga agtcgagatc
     7981 gcccgtcgtc acgaactgcg tgcgctggat gtcggtcagc atcggcatca gcagcgcttc
     8041 gatctgctcc ggggtcagcg tgcgctcgct ggccgaaacg agcttgccgc gcagtcggaa
     8101 catcaccgga tagcccggcg acaggtgcag gtcggatgca cctgcctgga tgagctgatc
     8161 gaagagcaca gcgatcgtca ttcgcgacgc cctcctcggc ccatcggctg cgccgccggt
     8221 tgcgcaggct gcttggcctg gttcgccgcc gtcggactgt attggcgcag cgtgtcttcg
     8281 gtgatcttgc ccgcacgcaa cagctcgagt agcgagtcgt cgaagcggat catgccgaac
     8341 gcgcggccgc gctgcatgag gttcggcagc tggaacagct tgtcgtcgcg gatcagcacg
     8401 gccagcggca acacgccggt cacgagctct atcgcgggca cgacgccgcc agcggtggag
     8461 ggtagaagcc gttgcgcgac gatcgccttc aacgcaccgg cgatcgatgc gcgtgcttgc
     8521 gtctgctcgt cgggcgggaa catgtcgatc aagcgatcga tcgtcttggc cgccgatggc
     8581 gtgctcatcg tcgcgagcac gaggtggccg gtctccgccg cggtgagcgc gatctcgacg
     8641 gtctcgcgat cgcgcagctc gccgatcacg atgacgtcgg ggtcctcgcg cagcgatgct
     8701 ttgagcgcgg cggcgaagct cttcgtgtga cggccgacct cgcgctgcga gatcacggac
     8761 tgcttgcgcg ggtgctcgag ctcgaccgga tcttcgatcg tgatgatgtg gtgcgccttc
     8821 gtcgagttga tgagatcgac gagcgcggtc attgtcgtcg tcttgccctg cccgttcggg
     8881 cccgcgatca cgacgagccc ttgatgatgc gctgtgacct tcgcgagctc gcgcggcagg
     8941 tcgagctcct cgagcgtcgg cggcgtcaca cgagcgagcc gaaacgcgcc gcgcagaccg
     9001 ccctgcaacc gcgagacgtt accgcgaagc cgacctgcac cttggacgtc gaacgcgagg
     9061 tcgacgtagc cgaccgtgcg cagccggacg cgacgacgcg gtccgagtag cggtaacagc
     9121 atccgatcga cgatgtcggc cggcaccgga gtcggatcga gcggacgcag ctcgcccatc
     9181 agacgcacca tggccgggcg tcccgcgacg atatgcagat cgctcgcgcc cttttgccgc
     9241 gcggcccgga cgtaccgctc gatccccctc gaatcgattt gctcgtcgaa gattgcgtcc
     9301 tcgccgagat cttcggccgt ctcgacgggg tcgggctgcg cctcgggttg cgcaggcgcg
     9361 gtggcctgta ccggctcgct gtcgtgcacc ggagcgccga gctcgacgcc ctcgtcgtcg
     9421 tccgcgggtt cggcgacagc cgcctggggc gtcgcgaact tcaccatcgg cacggcgaca
     9481 ggctctacag ccggcgctgg tgcggcagca ggcgcggcct ggatgcgcag cgcgctgccc
     9541 tcgggtcgcc tgacgagctc gacgcgaaca cgcctgcgac cgacatcgca atcgatcggc
     9601 tgcgcgcccg cggtcttgtg gatcttgtcg agcgccgtgc cgccgaccag cgtcgcgaac
     9661 tgcagctcgc tgaccggcag cgatgtcagc tgcatgtagc cctgcccggt cttgagcgcg
     9721 atcggacggc cgatcccgac gatgatctcg gtgacgtcgg cgcgatcgag gtagccgaga
     9781 agctgatcga gttttgcgct caagactagg gagctgagtt catgttgccg ttgccaccgc
     9841 acccgccctt ttgcgcgctg tagcagtgcg tgaccgtcgt gaaggtgatc agctcgcgca
     9901 cttcgcgcgg catgtcggga taccggaggc actgcgaaac gatatagatg cggctgtcga
     9961 catccgcgtt cgggttttca cccgacgctg gaatctcgtc gttgttgtcc tcgagggtga
    10021 cgcgccaatc gggatcatta ctggtgtccc cgtcgacgtc atggctgatg ttcgcgagcc
    10081 acgccggctc cgaagtcgac agcgttttgg gcagcccgag gtacgtaccc cacgtgtccg
    10141 agttctgcgc gaccgtctgg cgcgtcgcgg tcaggcccgc ttcggcgcag tacagcgcgc
    10201 tgatgccggt cttcgtgagc tcggtgttgc ggttcgagcc aagctgcaac gagacgagca
    10261 cggcggcacc cgcgagcaag gctgccatca ggatcatcgc gaccaacatc gcgctaccgc
    10321 gttcggagct tctcttcatg gtgaccccgc taggttgcga atctcgaccg tggtactgag
    10381 catgcggcga cgatacctat cggccgtggt cgaacctgga tggttcaacg cgtcgccggg
    10441 ctgatagaag ctggcggtgc ccgtgagggg cgcagtatcg cgcgagacga gcacgaccgt
    10501 caccgcacgg atcgtgccgg caggcagtgt atcggtggcc acgttgccgg cccactcgtc
    10561 gctggtggtg ccaccggtca aaaacggcga ggcgggcgtg agcgcgccgt cgctgttcga
    10621 gtcgacgcca accgcgatct gcatgtcctc gatgccctcg gcaagcggct cgtacgaact
    10681 cgagccgttc gggtacatga gcagcgtcgg gatgccgtcg acggcgccga cggtgaacct
    10741 cgcgcgttgg gcacgcaaga ggatcgagcc ctgcgggtag ccaccggccg gaaaggctgt
    10801 cgtacaggtg atcgtgtcga tggaaagcgc cgtggaggtg actccactca cccgagcgag
    10861 cgtgccttgg gtcgtatcgg tgacgaggac gtagtcgccg atggcgaact ggctgaggtg
    10921 cgccgcaggg atcgtgatgc tcgtcgacgt cgacgtgaac gcggagtgcg tcgtcgacac
    10981 gatgccgccg gaggcataga cgacggtcag ctcgtcaggc ccgatctcgc tgtcgacaaa
    11041 cgagatcgcc cccgagctga acgtgcagtc gtgcgcgtcc ttgatcgtgc cggtcgtgac
    11101 gcccggactc gcctggcgga tcgcgtcgac gatgaagtcg agtggcgcac ggaccgcgtt
    11161 ctcggtctgg accacgcggc gctgctcgcg gtgcgcgcgc gtcatcgcga cggcgaccga
    11221 cagcacgccg gcgatcgcga acgagaagat cacgagcgac accatcagct cgacgagcgt
    11281 gaagccgcgt tgcgcgcgag cgatttgttg cgcctcgcgg ttcacgtgct gcctcgctga
    11341 ccggtgacgg tcacagtgcg cggcacgcca tcctcgtccc actgcaccgt gacgatgatc
    11401 ttgaacacca ggggatccga cgtcgtcgcg atcgtatagg tgcgggtgaa aatcgtcgct
    11461 cccgtgagct ggtcgacgat tgctgggtcc tgcgtcggcc ctgatgcggc ggtcggcgct
    11521 gcctgcgtgc gcagctcttc gagcttgtcc tgcgccagca cggcggcctc ggtctcgcgg
    11581 cgcgagaagc tcgacgcgcg gctctcgacg cgatacaggc cgagcacgcc gatgaccgtg
    11641 agtgcggtca acagcatcgc caccatgacc tcgatcaggg tgaagccgga ctgctctgaa
    11701 cggcgggtta ctcggtccgg agccatagtg ctcgcctgag ttggttggag gttgccatcg
    11761 ggtcgaccgc ggcgccgctc gtgccgagcg catcggtcgt cagcttctcg ccgtaacggc
    11821 cgctgccggt gatcatcgtg gtgccattgt tgaacagcga tgccgcacca ctcgcgatga
    11881 tgagcgtcgt gccaagcgac ggcgtcgccg cgccaaagtc gtagttgtac gcgtgccccg
    11941 tcggcgtgct gctcgtaccg aacgcgtagt cgttgatgtt tgtcgtgtcg gtcgtcacga
    12001 acagctggtt accgacgacg gtcagctgcg agaagccggc ctcgccggca cccaggctga
    12061 acttgatcgg tagcttcgta aggtcggtct ccgcatcggt cagcgacgac gtgccggaat
    12121 aggacgtcgt caccgcaaac accatctgcg tcggcaccgg cgattcgttc tcgccgcgcc
    12181 acagcgtgag ctgcgagtcg gcgtagccgc cggtcgcgaa cgcggcgtac tgggtgccgt
    12241 tcttcgccag gatcgccggc ggtgcgccga tcgggtggta gtccgtggcg aggcgcatga
    12301 gcggcgtgtc cacacccgag accgtaccgt tctggttcgc accggtagcg gcgtcgcgca
    12361 cgaagacgtc gccgtacagc gtgccgtata cgaccttgtt gatgcgaccc gtgccggtcg
    12421 tgtcgaggcc caccgcgccg ccggggatgc cggcagacgg tgcaccctcg tgcgtgccgg
    12481 cgcgcgggct tggatagagg tcacccttct gccagatctt cgcgccggtc tcgacgtcga
    12541 tcgcggtgag cacgctcgcc gcgccgcccg tgccgccgtt ttgcgtctgc acataggcaa
    12601 cggtcttctt gacgccgctg atcgtcacgg tgcccgccgt gagattgagg ccgacgccga
    12661 gctcgtacgt gctgcggctg ccaaccgccg tcaccgtgta ctcccagagc acgcgcgggt
    12721 tgttcggcgc cgtcacgtcg atcgcataga ccgccggcac gacgttgttc gcggtcatgc
    12781 ggccggtacc agtgtggaag atcaagatcg tgtgccaggc gttgctgcca ttgttatcga
    12841 agtcgccgta cgcatcgatc acgttcggcg agccgtcgat gtgcgctgtg ctctgacgca
    12901 gatccggcag cacggtgcgc gggatgtacg cccacagctc gcggccaact tcgtcgcagc
    12961 cgccgccggt cgtcacgcag aacgcgtgca tcatgccgtc ggtcgcaccg acatacgcca
    13021 tcgtcgggcg gcccgcgctg ccggcaatcg tgctcggctc gatcacggca actgtcgagc
    13081 gatcgatgcc gccgagcttg gggatccagt tgttgctggc gtccttacgg ccggcaagga
    13141 tgcgcgagat gaacgtctgc ttctcggccg tcgtgaagtt cgacgcgagc gtgttgccga
    13201 gatcggtcgg cgcgctcgcc gtcgtcgcat tgtagttggc cgtcgtgaac atcacgtgcg
    13261 gcggcaggcg gcccgactgc agcgtggtga acacggtgcg gcagctgccg ttgaagttcg
    13321 tcgcgcagcc ggccgtattg accgtcggga tgtggtcggc cgcgtcgaac acgacgcctg
    13381 agaggctgct gatcgccgtg cgcgagccgt tgccggtgtc gcagcccgcg gcactgcacg
    13441 ctgcatagct cgcgaccgga atctggcgca ggtggccgag cgcgtacggg aacgtgaagc
    13501 gcgcgaggcc tgccgacgtt gtcgccttcg gaatgttcgg gatcgggtcg aagtgctcgt
    13561 acgtgccctg gaacacggta accgtgctgt cttcgtcggt cgcgacgatc ggaccgctgc
    13621 gcgagacctc gacgatggtc ggcgagctcg tgaacgtctc gtcggggagc gcgagcaacg
    13681 agttgagcag catgcgcagc tcggcgtagc taccgctgtt gttgagctgg ttgccggcca
    13741 ggtagtacgc gaggccgttg ttgagctgat ctttgaagcg cgcatacgtg gcgacgtcga
    13801 gcaggtcgcc ggtcgccgcg ctcggcgtga acgagtcgca gtccgtcgtc gattcgtgga
    13861 ggacgcacgc agtgttgttc gtgccggtcg tatcggccgt catcaggcgg cgcagcgacg
    13921 acgtgctcac ctggaacggg gtctggtagc cgacgttcgg cgaaccgttc ttgtagtact
    13981 tccagccctg caccgtaccg ttggcgctgg ccggaacgag gtcgcccgac tgcatgaaca
    14041 ggttgctcgg atagcgcatg atgaacggct cggtgttgcc gttgttctga atgtcgaggc
    14101 catcgccgga gctcggcttg ccgagcagct gcttgttcgg gcagccctcg gcaaccttga
    14161 tcgagtcgtc gaggaacacg ccgtggccgg cgttgcggat cgcagggagc gacgtcatga
    14221 agtcgcggac cttgtcgaac actgccttct cggccgagtt accgcacggg ctgttgtcgt
    14281 tgtaaccgaa cagccacagc cagccgaagc cgccggagac cagcgtgccc tgtgcgacgt
    14341 cggtgtcgtt gagcacgcag tacgtcgcgt ccgacggcga gaactcgccg ctcgcgcagc
    14401 tcgccgggct cgccggatcc tgaatgccag cggtgcctag ccagccctgc gcgacgttgt
    14461 tgttacagcc gacgcacgcg atgcgcggcg gctcgtagtc gatcttcacc gcgagcggcg
    14521 caccattgac cgtcgtgaac cgattcgagc tcgtgccgtc gcccgaggtg taatcctggt
    14581 agacgagctt cgccgacgga tcgagctcat agaggtcgat gccgctgttc gaccagttgt
    14641 acttcgtcgc gtcgagtgac gagtacgcgc cctggcgcag ccagaaatca tcgaagttcg
    14701 cgcgatcctc ggccgcgatc agaaaggtgc cgcctcgcac aggaaactgc ttgtactgat
    14761 agctgtccgt cttcggcacc agcgtcgcgc tcgtgaggcg gtgcacgggt tgtgtgcaac
    14821 ccgagccgca cgcgatcagc gggtcagcag aggtcggggg gtcggtgagg tcaccggtca
    14881 agacccacaa gtcgacatcg ttcgcgaggt acgtctgccc gcccgcgttc tgaccactgt
    14941 tgagtgcgac gatggcttta cccgggttga cgagccagta caccggaatc gcgcgctgca
    15001 tcaggcgata gacgaggccg tacgtcttga acatgccgtc gtcggccgcg ttgccgccga
    15061 tgcgcgtcgc cgatccgggc gcatccccgc accagccgtt gcccatgttg aacgtgagcg
    15121 ccttgccggt cgtgatcgcg gaaatgttgc cgctgctcgt cgtcgagtag gtgagagtcg
    15181 tgcagttgct gcagccgttc gtgacgacaa acgtgccgtt gagcgccgag ttgcccgtgc
    15241 ccacgatctt gacgtagtcg ccattgccgg cgccgtggtt tgcggtcgtc acgacggtga
    15301 ccgtcgaacc ggtccgcgag atcgacgaga tcgaccactc cttgcgcggc gggctcacgc
    15361 ccggcggacc gctcgtgccc gagccgatcg gctgatgaca gcggtccatc gcgatgatca
    15421 gcggatgatg cgtgcccgac gtcatgttga acgacgccgt gcagcaaccg ctgctcgtca
    15481 ccgtacaggc cgacagcggt gccgagcatt gggcgaagtc tgcgcgggcc tcgtgcgaac
    15541 gcacgacgag accgagcatg acgatgaggg ctaagcggct accaactttc gcgcgcatat
    15601 gagctccccg tgacgtggta gaccaaaact cgatattgct tcaccgtgct gttgcggtcg
    15661 gtgatgtaga tcgtcgacgc cgtcgccgaa ccatccggct tgaaatcgac gtagtacccc
    15721 ggcaagttgg tgtcttgcgc gggcgtcgcg ccggtcgatg tgacggcgct gtcttcggcg
    15781 ctccagatga tcgtgttctt cggcacgcgc ttgacctcga gcagcagcca gccggtgccc
    15841 gagtagctca tgcccgtcga aggcgcgacg tcgacctcga cgtactgctc gtcggtcgac
    15901 gtgtcggtgt cgaacacgac gcgatggact ttgcgcgtcg acaccgcgcg cgtgtgcgca
    15961 tagatgaacg tcgagttgat ctggctcgcg atcacttccg cgttcgcatc gtacggacgc
    16021 ggcgcaacac cgaacatcaa gccggcgagc accgcgatga tcgcgacgac gaccatcagc
    16081 tcgatgagcg tgaatccgcg ttgcgtgctg cgcgtcggtg attgcatcag tgaccgtccg
    16141 ccgctttgat cttggtgtcc cagctcttga agatgtaatc ggtgtcgggg cagtccgccg
    16201 tgatgaaata ccaagacagg gccggcgccg acggcggtgc acctgcccag gtgcctggcc
    16261 acgcgccgtc agccgtcggc tgatcgcccg cgttacctct tgcgatgaca tagctgcagg
    16321 tgagcttcga ctgcggcgcc tgcacgcgca gcagcgtcca cggcagcgtc gcggtcgtca
    16381 cgcacgtcgg cgtcgtcatg tccgtgcccg tcgagctcgc agtcggcgga cacgccgcca
    16441 tcgcgaggta cgcgctgttt tccaccttgt actgctcttc gcgcgtgctc agctccgcga
    16501 acatcggatc gacttcgctg cgcgacttgc cgcgcttcga ctccttgatg aacgacggaa
    16561 tcacgaccac cgcgaggatc gcgatgatcg cgacagcgat catgagctcg aggatcgtaa
    16621 agccacgttg cgtagctcgc tggtgcaccg aaccatgcta gcacgcagcc cctcgagacc
    16681 ggctctatct gtgggtcaac actgagttac ttgaccacct tgccgtcgac gtccagctcg
    16741 accaacccac ccgcgcccac actggttttc gcgagctcgg cgagcgcctc gcgcagcgtg
    16801 agttgcttgc cgtccttctc gtaaggcgct ttgcggtctt tgggcggagc gcccttcgga
    16861 cccttgtcga caatcatctt ttcgtcgcca ttaccgacga caacatagac cgcgcccttg
    16921 gtctggaggt acttcgtcgc cgacgccttg acctccttct cggtgacctt gcgcaccttc
    16981 gcgacgtacg agttgtagta gtcgagtggg agcccgtaat acacgaggct ccggtattgc
    17041 ccgagtgcag attgtgccgt cgagaagcgg gccggcatcg cgagctcggc gttctccttc
    17101 tcgcgcttca gctcctcggc ggtgaccggc gtcttgccag ccgccagctc cttcacctcg
    17161 cgatcgatct cgagcagcgt ctggtacgcc gaatccgctt gcacgccagc ggtcacgttg
    17221 accgtaccga ggcccttcag cggatacgag aacccgccgc gcgcaccgta cgagtagccc
    17281 ttgtcctcgc gcaggttcat gttgatgcgg ctcgtgaacc cgccgccgag cacggcgccg
    17341 agcatcgagt tcgcgaagta gtcgggtgcg ttgcgcttcg gtccaaacca catgagcgag
    17401 accgacgcct gcgccgcgtt cggaacgtgc acgaagaaga tcttgccctt catcgtcttc
    17461 gcggccggaa gcgctgcgac cttcggcgcg ctgcccgtcc acgaaccgag cggcgacttc
    17521 tcgaacgtct cgcgaacctg cgcttcggtg agatcaccga cgacgaacag gcgcgcgttc
    17581 ttcggcttca tccacgtcga gacttgcttc ttgcagtcgt cgagcgtgat cgactcgaga
    17641 ccgccctcgg tgatcacggc gccgagcgga tgatccgcgc cgtagagcac cggcccgctg
    17701 acgcggaacg ggatcgacgt cgggttcgtg cgcgactgcc gcaccgactc gatgcgccgc
    17761 ttcttcaagc gatcgaaatc cgagtcgcgc atgccgggcg ccaggatcgt gtcggcgaac
    17821 aacgcgaacg tggcgtcgac gtgcttcgtc aggctcgcga gctgaatgcc ctgcgagtca
    17881 tccgatgcat acgtgctgat gttcgacgcg gtgtcggcga gcgcttccga gtactggatc
    17941 ttgtcgagct tctgcgtgcc ctcggtcatg agtgacatgc agagaccggc gagaccgtcc
    18001 ttgccgttcg gatcggtcat gctgccgccg tcgaagttga ggtccatcga cacgatcggc
    18061 agcgtgtgct gctcgacgag gtagaccttg atgccgttct tcagcgtgaa cggcttgacc
    18121 ggcggtagct tgaagtcacg cgacgcgctc ggcgccggtt gcgtacgacg aaaatcctcg
    18181 tcggggaacg cgagctcttg cggttgagca gtttgcgaaa cagcaggcat cgtgttctcc
    18241 ggaggcttcg gtgtgaccgc gggggtcggc gttggcgacg gcggcaccgg agccggtgtc
    18301 gtcgtcttcg gagtcgagcc acaagcggcg agaacgagga acgcaggtac aagtgcgcgc
    18361 ttcacttggc acctcccgtc gggttcgtga tgatggtgac ggcgttcgcc ggcacgatgt
    18421 acttcgcagc cgccgcgcgg atcttgtccg gcgtggtctt gcggtagcga tcgaggtccc
    18481 aggtcagctt gcccggatcg ccgaggaagt ggttgtagct ctgcaacgtg ttcgcgcgcg
    18541 aattgacgtc ttcgagccgg tagatcaccg cggcctccgt cgctgcgaca aagcgcgaga
    18601 tctccttgtc ggagaggttc tccttcgtca cgcgctcgag ctcgtcgaac acgatcttct
    18661 gcaccgcctc gagatcggcc ccggtgcgca acgtgaccgt gacgctgaac tgaccggaga
    18721 actgcatgct gttctgaccg gcgcgaaccg atgtcgcgag ctgcttgtca tagacgagcg
    18781 acttgtagag tcggccgcgg ccctccgcgg tgagcgcgtt cgcgacgatg tcgagctcgg
    18841 catcgccgtc gccgaagttc gcgggtgatt gccacgccca cgtgacctgg cgctgcttcg
    18901 cgaagctgtc gtcgacgttc ttcgtcgctg ccttctgcgc gggcgctggc acgttcaccc
    18961 aggtcggctt cttgctggtc gggaagccgc cgaaccactt ctcgacgagt ttcttggtcg
    19021 cggcgacgtc gaagtcaccg acgattgcga gcgtcgcgtt cgacggtacg taccaggtct
    19081 tgaagaaatt cttcacgtcg tcgagtgacg ccgcggtgag atcctcgtgc ttgccgatcg
    19141 tcagatagcg atacggatgg ccttcgggat acatcgccgc atagagcgcg aacagcgcct
    19201 tgcgataggc gacgttgtcg tagttctggc ggcgctcgtt gcgcacgaca tcgatctggt
    19261 tgtcgagcgc agcgcgatcg accagctcga gcaagtgccc catgcggtcg ctctcgagcc
    19321 acagcccgac ttcgagttgg ttcgacggga tcacctcgaa gtagttcgtg cgatccgtgt
    19381 tggtcgtgcc gttgacgttg ctcgatccga tcttcttcag gatctcgaag tgcttgtcgg
    19441 agccgacgtt cttggagccc tgaaacatca tgtgctcgaa caggtgcgcg aagccgctct
    19501 tgccgacgac ttcgttgccg ctgccgacgt ggtaccagac gttgaccgcg acgagcggca
    19561 cgctgttatc cggcgcgagg atcacctcga gcccgttcgg cagcttgtac ttctcgaatt
    19621 ccagcttggg atcgtccgca aacgcgggtg cgctgaccgc gagcacggca gcgacgacga
    19681 gcaatggcct catgggccgg gaccctagcc tagagctcga gcggctgcac gccgaggact
    19741 acgtgatcct tcgagacagc gtctagccat aggagcaacc tgcgcgtctc ggtcgccgac
    19801 ac
//
LOCUS       DASUFX010000691        10343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1083116, whole genome shotgun sequence.
ACCESSION   DASUFX010000691 DASUFX010000000
VERSION     DASUFX010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083116"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFV99_27880"
     CDS             <1..643
                     /locus_tag="VFV99_27880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063240.1"
                     /translation="SLGHFLDELDRKVGKGRWAMILTSDHGGSPLPETLNGGRFTFEQ
                     IQRAANQAASLELGAGDWIAYVGEPQVYLSQAALAQDPKSLKAAMKKILFALRAFPGI
                     AIADRTDAVTGDCDKRDGDDRALCLTLDRERSGEFFFMPAKGWIMEEEDERLATAHGS
                     LNDYDRNVPVVLLPFDRKPHDPVAAPGPELSLAEIAPLIASWLGVQSPTQLPR"
     gene            complement(665..970)
                     /locus_tag="VFV99_27885"
     CDS             complement(665..970)
                     /locus_tag="VFV99_27885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063241.1"
                     /translation="MDDEARRRLIAEATVQNAREEAKRARVNMPIGLALVAFGVTLST
                     LAAVVPLGLIGTVVLAGIGFVFIPGGLAVIVRAASTIARANRALREHQLPAARTLKS"
     gene            complement(1012..2520)
                     /locus_tag="VFV99_27890"
     CDS             complement(1012..2520)
                     /locus_tag="VFV99_27890"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HEX5063242.1"
                     /translation="MVASLDLDPTIGALLGETTTEIDVARKNRVFCNRNLRMDSIEMI
                     GFDMDYTLALYHQEKLEQLSIALTLSKLCEKHGYPESIRELTYDPRWAIRGLMVDRLN
                     GNVFKMDRHAYVGRCYHGFRELDHDQRRAAYRNEKINLSSDRFEWIDTLFGLPEAVMY
                     TTLVDWADRQTGTVDYDKLFGDIRTAIDEAHRDDTLKSVIKADLPAFIVKDPLLGETL
                     HKFRSSGKKLFLLTNSLWDYTSVVMSYLLDGERKAYPSWRNYFDIVIVGGAKPAFFNE
                     VRPFIQIDPATGEQITNGEIKHLSRDKVYQAGNVIAFEQMTGSRGDQVLYIGDHIYGD
                     ILRLRKGHMWRTAMVLQELEREITVSERLEGQIRDLDLLDRRHRNLESEIDYQTLRLK
                     KIQRLLDDPTTSAALRARLEEERKNARTNIDSLRDRANLMTEEVDSLESRIDRAYNSH
                     WGSCLREGNENSRFGEQVNDYADLYTSRVSNFGPYSPLRYFRAPRRPMPHEM"
     gene            2560..3114
                     /gene="hpt"
                     /locus_tag="VFV99_27895"
     CDS             2560..3114
                     /gene="hpt"
                     /locus_tag="VFV99_27895"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5063243.1"
                     /translation="MPSPPSWNREHEPFAELISAEAIAARVSELGAQITAEYRGRADV
                     VVIGVLKGSVIFLSDLVRHIALPIVVDFIGISSYGDATVSSGVVRITQDLSRPIEGKH
                     VIVVEDIVDTGHTVHYLLENLGTRRPASIKLASLLHKPERQERAVTIDYLGFTIPNKF
                     VVGYGLDVGQHYRNLPFIGYVTNA"
     gene            3111..4109
                     /locus_tag="VFV99_27900"
     CDS             3111..4109
                     /locus_tag="VFV99_27900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5063244.1"
                     /translation="MKPEQLERLPAVLFEIGKLIGSDLDPGILLSRISELICQLVNAK
                     ACSVMLLDASRKRLLARAAYGLRTERMHTLSFAIGEGVAGWVVERGEPALIQDVSKDP
                     RFVVLPANQTPIASMLCVPLLSRGERVGVVTATAEHTDAFDASDLDLVRFIATTIALD
                     IENVRLHRVAVTDPLTGIYNREFLQQRLPAEIEATIDRDRPLSIALVDVDHFKAVNDQ
                     YGHGTGDVVLAEVARRLRSAIRGGDVLVRYGGEEFIAVLPKADAGRAWEVGERMRQRV
                     CERAFDVGDGLALLLRISVGIAQWRMGEDMADLIERADIALYGAKQRGRNRVEVAP"
     gene            4205..5383
                     /locus_tag="VFV99_27905"
     CDS             4205..5383
                     /locus_tag="VFV99_27905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063245.1"
                     /translation="MQPRPAAPGEVSPAALEPPAASVEVSPAAFAPAVPTADPPSGDM
                     RPLDTELQPPGSPTPNDIATSGEYVATPIPPAAGDIALAGGPIPDEPILPTSLDDNAL
                     RDAVGAPRPAPRPKRAKTERLDDDGLPKPGGRKTLVIAAVAAVVGLGIAALVLLGRVS
                     SARYVIACSTTQVTAEQGRSFPPWGSRPLTGPEWKPIALPANAECKPRETDDIGELGR
                     WYLDVLVDRASTTLTARDLLDSIQPNKPNPLDFAAEQLNQALLLSRSPDRRDQRKEVE
                     RLLGDVQYWRASVRLRDASAALVDASRQFDAAAAQRPRHVTDAAEWATFLRRLAEELR
                     AGPAGVQAVVTPGPQPGPQPGEHVTAPMGTALPVEPEGSGSEPVETPDAGLPTGGVLL
                     "
     gene            5444..6016
                     /locus_tag="VFV99_27910"
     CDS             5444..6016
                     /locus_tag="VFV99_27910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5063246.1"
                     /translation="MARNIAMLVGSLRKDSFTRKVSNALIELAPPSLQIEHVAIGTLH
                     LYNQDLEAEPPPEWLAFRDRIRPMDGVIFATPEYNRSIPGGLKNAIDVGSRPYGKSVF
                     AGKPAAVISVSPGQIGAFGANHHLRQSCVFLDMPVMQQPEAYISHVDKLLDENGKVIE
                     KSRGFLKQFITAYAAWVELFATATQQRSAA"
     gene            6039..7028
                     /locus_tag="VFV99_27915"
     CDS             6039..7028
                     /locus_tag="VFV99_27915"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5063247.1"
                     /translation="MRLLVIGVVFLACNHGSKEKPKPQTQTERAATEAQGSAATDPWA
                     AEPKSNPDDPPTFTERHKLADAACPTVKGPYFYKIEKGGKVSYILGTRHLGVSLDKFP
                     KPVHDAISSAKLAVFEVAPGDDSELPEQKVEVRDVLGPELWTHYQELVGKQTADALEN
                     TQPSVAVVSMMAMYEDISATLDLEIEHKVQDAKIPTQGLETAEFQDRLLNKVLDVRML
                     RAAVQQTKDRKELEKESRDDLTEYCAGTDDKPGMDDDQRKDMLAAGYTQAELDKIDEE
                     MVYARNADWIPKLEKILAKGDVFIAVGADHLSGPKGVVALLQKRGYTLKRITQ"
     gene            complement(7055..7396)
                     /locus_tag="VFV99_27920"
     CDS             complement(7055..7396)
                     /locus_tag="VFV99_27920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEX5063248.1"
                     /translation="MTNLACPICSLEDVLSRADRYECATCGHEWPREAPVEVAEVEVA
                     RVVKDANGNVLADGDSVTLIKDLKLRGSSEVLKQGTKVKNIRLVDGDHEIDCKIDGSA
                     MALKACFVKKA"
     gene            7489..8859
                     /locus_tag="VFV99_27925"
     CDS             7489..8859
                     /locus_tag="VFV99_27925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5063249.1"
                     /translation="MRIRFDKGTLVIDRVESGLEHVQLLGGRLDQELVAWRLPAEKLS
                     ELRGRLSAGHVRFTDTIEPTALEPRWSLPELRWYQREAVTAWRDAGDRGVVVLPTGAG
                     KTLVALAAIAELGVSTLIVVPTRLLLDQWGRALEAAWPHPVGRLGDGDYRVAPITVAT
                     YASAVTWAPHIGDRFGLVVVDEAHHVGAWCPSEILEMLVAPSRLGLTATPSQAAALPR
                     HIGPLVYDLTIEDLRGEALADYTIETVDIELTPDERSRYSLHRSEFAKFYAQLMRIDP
                     GTSWRDFMSVARRSERGRDALSAWRASRALLAYPDAKRRAVRDLLARHATERTLVFTA
                     DNTTAYAIARELLVPPITCDIGRVERKRMIDRFRAGETTVLVSAQVLDEGFDLPEAEV
                     AIVVGGTGSTRRHVQRIGRLLRPRPGKRALVYELVVQRSAEVSQMTRRRRGLTHDDAP
                     TMEAMP"
     gene            8856..9965
                     /locus_tag="VFV99_27930"
     CDS             8856..9965
                     /locus_tag="VFV99_27930"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX5063250.1"
                     /translation="MSTLTVLDETDFDWIGEALDVVVAATGQPWRVALEHLDDARRSE
                     QSRAPTRFAAVVNAIQRLLGGRARNANAARTARTLVLGKPTLTAAEREARIAYAAGEL
                     EITCAAVETMLWSDLPRERPVELPRGRPAERDVAAHANLSLVQRAMMRAQTVELRVRG
                     DAGPLIHTAAARGLLTTVSRGDDGNTMLDIVGPLMLFHRTGVYGRALASLMPLLAECE
                     GFELSLLSRSREQLYPVDIASPVLLPPVPANMQVPDTRLLRLLKELKRAAHDIVVTLR
                     PHPISAGRSLVCPDLGLERDGRVTFVELVGFWTSEFLVKKLELYGDAGINDVVFCVDE
                     ARSVANETLPDGLPIVRYGRRMADTAAELVARVMS"
     gene            complement(9971..>10343)
                     /locus_tag="VFV99_27935"
     CDS             complement(9971..>10343)
                     /locus_tag="VFV99_27935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5063251.1"
                     /translation="RNRNRSHPGDRANLTRTPRLRGWRASPSTRSGKRLVPIIERSDR
                     ELGDQVRRAASSIVLNLAEGQRSAKGNKHKHYSIAHGSANEVKAALDVACAWGWIGEA
                     HEPQRILDRLLAILWRLTHPR"
BASE COUNT         1755 a         3523 c         3318 g         1747 t
ORIGIN      
        1 gagcctcggg cattttctcg acgagctcga ccgcaaggtc ggcaagggcc gctgggcgat
       61 gatcctgacc agcgaccacg gcgggagccc gttgccggag acactgaacg gcggccgctt
      121 tacgttcgag cagatccagc gcgccgcgaa ccaggccgcg tcgctcgagc tcggcgcggg
      181 cgattggatt gcctatgtcg gtgagccgca ggtgtatctc tcgcaggcag ctctcgcgca
      241 ggatcccaag tcgctcaagg ctgcgatgaa gaagatcctg ttcgcgctgc gcgcattccc
      301 gggcatcgcg atcgccgatc gcaccgatgc cgtgaccggt gattgcgaca agcgcgacgg
      361 cgacgatcgc gcgctgtgcc tgacgctcga tcgcgagcga tcgggcgagt tcttcttcat
      421 gcccgcgaag ggctggatca tggaagaaga agacgagcgc ctcgcaaccg cgcacggctc
      481 gctcaacgac tacgaccgca acgtgccggt cgtgctgctg ccgttcgatc gcaagccgca
      541 tgacccggtc gccgcgcccg gccccgagct gtcgcttgcc gagatcgcgc cgctgatcgc
      601 gagctggctc ggcgtgcagt cgccgacgca gctgccgcgt tagttgtaca agtagtgcct
      661 gacgctagct cttcagcgta cgcgccgccg gcagctggtg ctcgcggagc gcgcggttcg
      721 cacgcgcgat cgtcgaagcc gcccgcacga tcacggcaag cccacccgga atgaacacga
      781 agccaatgcc ggcgagaacc accgtgccga tgagcccgag cggcacgacg gccgctagcg
      841 tcgagagcgt gacgccgaat gcgacgagcg cgaggccgat cggcatgttc acgcgcgcac
      901 gcttggcctc ttcgcgcgcg ttctgtaccg tcgcctccgc aatcaatcgc cgccgcgctt
      961 cgtcgtccac gccgcgactc tacctcaatg ccgtcgccgc gctacagcgc gctacatctc
     1021 gtgcggcatc ggccggcgcg gcgcccggaa gtaacgtagc ggagagtacg gaccgaagtt
     1081 cgagacgcgc gacgtataga ggtccgcgta gtcgttgacc tgctcgccga agcgcgagtt
     1141 ctcgttgcct tcgcgcaggc agctgcccca gtgcgagttg tacgcgcgat cgatgcgcga
     1201 ctcgagcgaa tcgacttcct cggtcatcaa gtttgcgcgg tcgcgcagcg agtcgatgtt
     1261 cgtgcgcgca ttcttgcgct cttcttcgag gcgcgcgcgc agcgctgctg acgtcgtcgg
     1321 atcgtcgagc aggcgctgga tcttcttgag ccgcagcgtc tggtagtcga tttccgactc
     1381 caggttacgg tgtcggcgat cgagcagatc gagatcgcga atttgacctt cgagtcgttc
     1441 gctcaccgtg atttcgcgct cgagctcctg cagcaccatc gcggtgcgcc acatgtgtcc
     1501 tttgcgcagg cgcaggatgt cgccgtagat gtggtcgccg atgtagagca cctggtcgcc
     1561 gcggctgccg gtcatctgct cgaacgcgat cacgttgccg gcttgataga ccttgtcgcg
     1621 cgacaggtgc ttgatctcgc cgttcgtgat ctgctcgccg gtcgccggat cgatctggat
     1681 gaacgggcgc acctcgttga agaacgccgg ctttgcaccg ccgacgatca cgatgtcgaa
     1741 gtagttgcgc cacgaggggt acgccttgcg ctcgccgtcg agcaggtagc tcatcacgac
     1801 gctcgtgtag tcccagagcg agttggtgag caggaacagc ttcttgcccg agctgcggaa
     1861 tttatggagc gtctcgccga gcagcggatc cttgacgatg aacgccggca ggtccgcctt
     1921 gatcacgctc ttcagcgtgt cgtcgcggtg ggcttcgtca atggcggtcc ggatatcccc
     1981 gaacagcttg tcgtagtcga cggtgccggt ctggcgatcc gcccagtcga cgagcgtcgt
     2041 gtacatgacg gcttcgggca gaccgaacaa cgtgtcgatc cactcgaacc gatcgctcga
     2101 caggttgatc ttctcgttgc ggtacgccgc gcgccgctgg tcgtgatcga gctcgcggaa
     2161 gccgtggtag cagcgaccga cgtacgcgtg gcggtccatc ttgaacacgt tgccgttcaa
     2221 gcgatcgacc atcaaaccac ggatcgccca ccgcgggtcg taggtgagct ccctgatcga
     2281 ctccgggtag ccgtgctttt cgcagagctt ggacagggtc agggcgatgg agagctgctc
     2341 gagcttctcc tggtgataga gcgcgagcgt gtagtccatg tcgaacccga tcatctcgat
     2401 cgagtccatc cggaggttcc gattacagaa cacgcggttc ttgcgggcga cgtcgatttc
     2461 ggtggtcgtc tcgccgagaa gtgcgccgat cgtcggatcg agatcgaggc ttgcgaccat
     2521 tcgacattat tgtagcgcgg ttgcgataca ttcggggata tgccctcgcc accctcctgg
     2581 aaccgtgaac acgagccctt tgccgagctg atttcggctg aggcgattgc cgcccgcgtc
     2641 agcgagctcg gggcgcaaat cacggccgag taccgcgggc gtgccgatgt cgtcgtcatc
     2701 ggcgtgctga aaggctccgt tatcttccta tccgacctgg tccggcatat cgcgctgccg
     2761 atcgtcgtcg atttcatcgg catctcgtcg tacggggacg cgaccgtgtc atcgggcgtg
     2821 gtccgaatca ctcaggactt gtcgcggccg atcgagggca aacacgtcat cgtcgtcgag
     2881 gacattgtcg acacgggaca taccgtgcac tacctactgg aaaatctcgg cacccgccgc
     2941 ccggcgagca tcaagctcgc ttcgctactt cataagcccg aacggcaaga gcgtgcggtt
     3001 accatcgact atctggggtt cacgattccc aacaagttcg tcgtcggcta tgggctcgac
     3061 gtcggacagc actaccgcaa cctgcccttc attggttacg tgacgaacgc atgaaaccgg
     3121 agcagctcga acggctccca gcggtcctct tcgagatcgg gaagctgatc ggatcggatc
     3181 tcgatccggg aattctattg tcgcgcatct ccgagctcat ctgccagctc gtcaacgcga
     3241 aggcgtgctc cgtcatgctg ctcgacgcga gccgcaagcg cctgctcgcg cgcgccgcct
     3301 acggcctgcg caccgagcgc atgcacacgc tgtcgttcgc gatcggcgaa ggcgtcgcgg
     3361 gctgggtcgt cgagcgcggc gagcccgcac tgatccagga cgtctccaag gacccgcggt
     3421 tcgtggtgct gccggcgaac cagacgccga tcgcgtcgat gctgtgtgtc ccgctgctct
     3481 cgcgcggcga gcgcgttggc gtcgtcaccg cgacggccga gcacaccgac gcattcgacg
     3541 cgagcgacct cgacctcgtg cgcttcatcg cgacgacgat cgcgctcgac atcgagaacg
     3601 tgcgactgca ccgcgtcgcg gtcaccgatc cgctgaccgg aatttacaac cgcgagttcc
     3661 tccagcagcg actgccggcg gaaatcgaag ccacgatcga tcgcgaccgc ccgttgtcga
     3721 tcgcgctcgt cgacgtcgac cacttcaagg ccgtcaacga ccagtacggc cacggcaccg
     3781 gcgacgtcgt gctcgccgag gtcgcgcgcc ggctgcgcag tgcgatccgc ggcggcgatg
     3841 tgctcgtgcg ctatggcggc gaagagttca tcgcggtgtt gccgaaggcc gatgcggggc
     3901 gcgcgtggga ggtcggcgag cgcatgcgtc agcgcgtgtg cgagcgcgcg ttcgatgtcg
     3961 gcgacggact cgcgctcttg ttgcgcatct cggtcggcat cgcgcagtgg cggatgggcg
     4021 aggacatggc cgatctgatc gagcgcgcgg acatcgcgct gtacggcgcc aagcagcgcg
     4081 gccgcaatcg cgtcgaggtt gccccgtgac cgacgacgac cggcctccgc gctccggtcg
     4141 tcgacggccc ccgcaggaac cgacagcgcc atcgttaccg acaccattcg agccacccga
     4201 tttggtgcaa cccagaccgg ccgcgccggg cgaggtttcg cccgctgcat tagaaccgcc
     4261 ggccgcgtcg gtcgaggttt cgcccgctgc gttcgcgccc gcagtgccga ccgccgatcc
     4321 gccgagcggc gacatgcggc cgctcgatac cgagctccaa ccgcccggct cgccgacgcc
     4381 aaacgatatc gcgacgtccg gcgagtacgt cgcgacgccg attccaccgg ctgctggcga
     4441 tatcgcgctc gcgggcggcc cgatacccga cgaaccgatc ttgccgacgt cgctcgacga
     4501 caacgcgttg cgcgatgccg tcggcgcacc gcggccggcg cctcggccga agcgtgcgaa
     4561 gaccgagcgc ctcgacgacg atgggctgcc aaagcccggc ggtcgcaaga cgctcgtgat
     4621 cgcggcggtc gcggcggtcg tcggcctcgg catcgccgcg ctcgtgctgc tcggccgcgt
     4681 gagctcggcg cgctacgtca tcgcgtgctc gacgacgcag gtcaccgccg agcaaggccg
     4741 tagctttccg ccgtggggct ctcggccgct caccgggccg gagtggaagc cgatcgcgtt
     4801 gcccgcgaac gccgagtgca agccgcgcga gaccgatgac atcggcgagc ttggccgctg
     4861 gtacctcgat gtcctcgtcg atcgcgcgag cacgacgctc accgcgcgcg atctgctcga
     4921 cagcatccag ccgaacaaac ccaacccgct cgacttcgcc gccgagcaat tgaaccaggc
     4981 gctgctgctg tcgcgttcgc ccgatcgccg cgatcagcgc aaggaagtcg agcggctgct
     5041 cggcgatgtc cagtactggc gcgcatcggt gcggctgcgc gatgcgtcgg cggcgctcgt
     5101 cgatgcgtcg cgccagttcg atgcagcggc agcacagcgg cctcggcacg tcaccgacgc
     5161 cgcggaatgg gcaacgttcc tacgccggct cgccgaggag ttgcgtgcgg ggccggccgg
     5221 tgtgcaggcc gtggtcacgc cgggcccaca gccgggtccg cagcccggcg agcacgtgac
     5281 cgcgccgatg gggaccgcac ttcccgtcga acccgaaggc agtggcagcg agccggtcga
     5341 gacgccggac gcggggctgc cgaccggcgg cgtgctgctg tagctgactc gcgcgtagtc
     5401 gcgagttggc cacgcgtctg cactcctccg agtcggagga accatggcac gcaacatcgc
     5461 aatgctcgtc gggagtcttc ggaaggactc cttcactcgc aaggtcagca acgcgctgat
     5521 cgagctcgca ccgccgtcgt tgcagatcga gcatgtcgcg atcggcacgc tgcatctcta
     5581 caatcaagac ctcgaggcgg agccgccgcc ggagtggctc gccttccgtg atcgcatccg
     5641 gccgatggac ggcgtcatct tcgcgacgcc cgagtacaac cgctcgattc ccggcggcct
     5701 caagaacgcg atcgacgtcg gctcgcgtcc gtacggcaag agcgtgttcg ccggcaagcc
     5761 agcggcggtg atcagcgtgt cgcccggcca gatcggtgct ttcggggcca accatcactt
     5821 gcggcagtcg tgcgtgttcc tcgacatgcc cgtgatgcag cagcccgagg cgtacatttc
     5881 gcatgtggac aagcttctcg acgagaacgg caaggtcatc gagaaatccc gcgggttctt
     5941 gaagcagttc atcaccgcgt acgccgcctg ggtcgagctg ttcgcgacgg caacccagca
     6001 gcgctcggcg gcctgattct gcgctaccgt actgagagat gcggctcctc gtcatcggcg
     6061 tggtgttcct cgcctgtaac cacggttcga aggaaaagcc gaagccgcag acgcagaccg
     6121 agcgcgcggc gaccgaagcg caaggcagcg ccgcgaccga tccatgggca gccgaaccga
     6181 aatcgaaccc ggacgatccg ccgacgttca ccgagcgaca caagctcgcc gacgccgcat
     6241 gcccgacggt caaagggccc tatttctaca agatcgagaa gggcggtaag gtctcgtaca
     6301 tcctcggcac gcgtcatctc ggcgtgtcgc tcgacaagtt tcccaaaccg gttcacgacg
     6361 cgatcagctc ggcgaagctc gccgtgttcg aggtcgcgcc cggcgacgat tccgagctgc
     6421 ccgagcagaa ggtcgaagta cgcgacgtgc tcggtcccga gctgtggacg cactaccagg
     6481 agctcgtcgg caagcagacc gcagacgcgc ttgaaaacac ccagccaagc gtcgcggtcg
     6541 tatcgatgat ggcgatgtac gaggacatca gcgcgacgct cgacctcgaa atcgagcaca
     6601 aggtgcaaga cgcgaagatc ccgacgcagg gcctcgagac agcggagttc caggatcggc
     6661 tgctgaataa agtgctcgac gtccgcatgt tgcgtgcggc ggtccagcaa accaaagatc
     6721 gcaaagagct cgagaaggaa agccgcgacg atctcaccga gtactgcgcg ggcaccgacg
     6781 acaaacccgg catggacgac gaccagcgca aggacatgct cgcggccggt tacacgcagg
     6841 ccgagctcga caagatcgac gaggaaatgg tctacgcgcg caacgccgac tggatcccga
     6901 agctcgaaaa gatcctcgcg aagggcgacg tcttcatcgc ggtcggcgcc gatcacctga
     6961 gcggccccaa gggcgtcgtc gcattgctcc agaagcgtgg ctacacgctc aagcgcatca
     7021 cgcagtagcg tgcggagcgc tgacagacgg cgaatcaggc cttcttcacg aaacaagcct
     7081 tgagcgccat cgcgctgccg tcgatcttgc agtcgatctc gtggtcacca tcgaccaagc
     7141 gaatgttctt gaccttggtg ccttgcttca gcacttccga cgagccgcga agcttcagat
     7201 ccttgatcag ggtcacgctg tcgccgtcgg cgagcacgtt tccattggcg tccttcacga
     7261 cgcgcgcaac ttcaacttct gcgacctcga ctggcgcctc gcggggccac tcgtggccac
     7321 acgtcgcgca ctcgtagcga tcggcgcggc tcaacacgtc ctctagcgaa cagatgggac
     7381 aagcgaggtt cgtcatcgtg ccgcgcatcc tacgagcacc agtcgctcgg cgccagcccg
     7441 gcgtctcctc accgcggagc gggcacgtga cttgctgtgc gccactgcat gcgtattcga
     7501 ttcgacaaag gaaccctcgt gatcgaccgc gtggagagcg gcctcgaaca cgtgcaattg
     7561 ctaggcggta ggctcgatca agagctcgtc gcgtggcgtc tcccagcaga gaagctgtcc
     7621 gagctgcgtg gccgactttc ggccggacac gtccggttca cggacacgat cgagccgacc
     7681 gcgctcgagc cacgctggtc gttgcccgag ctccgctggt accaacgcga agcggtcact
     7741 gcctggcgtg acgcgggtga tcgcggtgtc gtcgtgctgc cgaccggcgc gggcaaaaca
     7801 ctcgtcgcgc tcgcagcgat cgccgagctc ggtgtcagca cgctgatcgt cgtgccgact
     7861 cgcctgctcc tcgatcaatg ggggcgcgcg ctcgaggccg cgtggccgca tccggtcggc
     7921 cgcctcggcg acggcgatta ccgcgtcgca ccgatcacgg tcgccaccta cgcaagtgcc
     7981 gtcacgtggg caccgcacat cggcgatcga ttcggcctcg tcgtcgtcga cgaagcgcat
     8041 cacgtcggtg cgtggtgccc gtccgagatc ctcgagatgc tcgtcgcgcc atcgcggctc
     8101 ggcctcaccg caacaccctc gcaggccgcc gcccttcccc gccacatcgg tccgctcgtc
     8161 tacgacctca cgatcgaaga cctgcgcggc gaagcgctcg ccgactacac gatcgaaacc
     8221 gtcgacatcg agctcacgcc cgacgaacgt tcgcgctact cgctccaccg cagcgagttc
     8281 gccaagttct acgcgcagct catgcggatc gatccaggca cgtcgtggcg cgacttcatg
     8341 agcgtcgcgc ggcgcagtga acgcggccgc gatgcactgt ctgcatggcg tgcgtcgcgc
     8401 gcgctgctcg cgtaccccga cgccaaacgt cgcgccgttc gcgacctgct cgcccgccac
     8461 gcaaccgaac gcacgctcgt cttcaccgcc gacaacacga cggcctacgc gatcgcacgc
     8521 gagctgctcg tgccgccgat cacgtgcgac atcggccgtg tcgagcgcaa gcgcatgatc
     8581 gatcgcttcc gcgcaggcga aacaacggta ctcgtgtccg cccaggtcct cgacgagggg
     8641 ttcgatcttc ccgaggccga agtcgcgatc gtcgtcggtg gcaccggcag cacgcggcgc
     8701 cacgtccagc gcatcggtcg cctgctccga ccccgtcccg gcaagcgggc gctcgtctac
     8761 gagctcgtcg tccagcgcag tgccgaggtg tcccagatga cgcgccgccg ccgcggtctc
     8821 acccacgatg acgctccaac gatggaggca atgccatgag cacgctcact gttctcgacg
     8881 aaaccgattt cgactggatt ggcgaagcgc tcgacgttgt cgtcgccgcc accggccagc
     8941 cgtggcgcgt cgcgctcgaa cacctcgacg acgcgcgccg ctccgaacaa tcacgcgcgc
     9001 ccacccgctt tgccgccgtc gtcaacgcga tccagcgcct gctcggcggc cgtgctcgca
     9061 acgcgaacgc cgcacgcacc gcgcgcactc tggtgctcgg caagccaacg ctgaccgccg
     9121 ccgaacgcga agcgcgcatc gcatacgcgg ccggcgagct cgagatcacg tgcgccgcgg
     9181 tcgaaacgat gctgtggtcc gacttgccgc gcgagcgccc ggtcgagctg ccgcgcggtc
     9241 ggcccgccga gcgcgatgtc gccgcgcacg cgaacctcag tctcgtccag cgcgcgatga
     9301 tgcgtgcgca gaccgtcgag cttcgcgtcc gaggcgatgc cggcccgctc attcacaccg
     9361 ccgctgcacg cggcctgctc actaccgtct cgcgcggcga cgacggcaac acgatgctcg
     9421 acatcgtcgg tccgctgatg ctgttccatc gcacgggcgt ctacgggcgc gcgctcgcga
     9481 gcctgatgcc gctgcttgcc gagtgcgaag gcttcgagct gtcgctactg tcgcgcagtc
     9541 gcgagcaact ctaccccgtc gacatcgcat caccggtgtt gttgccgccg gtgcccgcga
     9601 acatgcaggt gcccgacaca cgcctgctgc gactgctcaa ggagctgaag cgcgccgcac
     9661 atgacatcgt cgtcacgctg cggccgcatc cgatcagcgc tgggaggtcg ctcgtatgtc
     9721 cagatctcgg cctcgagcgc gacggccgcg tgaccttcgt ggaactcgtc gggttctgga
     9781 ccagcgagtt cctggtgaag aagctcgagc tttatggcga cgccgggatc aacgacgtcg
     9841 tgttctgcgt cgacgaagca cgcagcgtcg caaacgagac gctgccagac gggctaccga
     9901 tcgtgcgcta cggacgccgc atggctgaca cagcagcgga gctggtggcc cgagtcatga
     9961 gctaacagca tcatcgtgga tgcgttaggc gccagaggat cgcaagcaga cgatcgagga
    10021 tcctttgcgg ctcgtgtgct tcgccgatcc agccccaagc gcaagccacg tcgagcgcgg
    10081 ctttgacctc gttcgcgctg ccgtgcgcga tcgagtagtg cttgtgcttg ttgcccttcg
    10141 ccgatcgctg gccttcggca agattcaaca caatgctgct cgcagcgcgc cgcacctgat
    10201 caccgagctc gcgatcactg cgttcgatga tcggcacgag ccttttgccg gagcgagtgc
    10261 tcggcgaggc ccgccagcca cggaggcgag gcgttcgcgt caggttcgcc cgatccccgg
    10321 ggtggctgcg gttgcggttc ctg
//
LOCUS       DASUFX010000692         8229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_388378,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000692 DASUFX010000000
VERSION     DASUFX010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388378"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2916
                     /gene="purL"
                     /locus_tag="VFV99_27940"
                     /gene_synonym="purI"
     CDS             <1..2916
                     /gene="purL"
                     /locus_tag="VFV99_27940"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX5063252.1"
                     /translation="EGATGRGAKPKAGLVGFTVSDLRIPDALEPWERDEWIGSPARIA
                     TALDIMTEGPLGGAAFNNEFGRPAILGYFRTFELGDRGYHKPVMLAGGVGTVRAQHVQ
                     KAKVPAGAALVVLGGPAFLIGLGGGAASSLAQGASAEDLDFASVQRDNAEIQRRCQEV
                     IDRCWALGDKNPILSIHDVGAGGLSNAMPELVHDAGLGAKLELRKIPTGEPDLSPLEL
                     WSNEAQERYVMAIGQERLAEFAALCARERAPWAQLGIATPDGRLTLADERGGPPPVDL
                     PLEVVLGKPPRMTRRAEPLPSPRRALDLAGASVADALERVLGLPTVADKTFLISIGDR
                     TVGGMVSRDPMIGRFQVPVADCALTTAGFDTYAGEVMALGERPPVALLDAAAASRLAI
                     AEAITNLAGAPIGTAPSALGRIKLSCNWMAAAGHPGEDARLYAGVKAAAETAVALGIA
                     IPVGKDSMSMRTQWDGKAVVSPVTLVVTAFGPVEDVRKNITPELRGTGELLLVDLGAG
                     QGRLGASCLAQVFGQLGDVPPDLDDPTKLRGFFATMQSLVADGKLLAYHDRADGGAIV
                     TALEMAFASGLGLELDLTALHADAFRALFAEELGAIIEVTREHVDAVRAAFAKHNIGV
                     HAIGRAVAGEQIRVSHGGALVLDASRSELRARWSHVTHQIARRRDDSACADEEHATRI
                     DAANPGIATKLTFDPAQDIAAPFIAKGARPRVAILREQGVNGQIEMAAAFTRAGFDAV
                     DIHMTDLIEGRADLSDCRGAIACGGFSFGDVLGAGRGWATTFRYNARAQSAIRRFADR
                     HDTFVLGVCNGCQMVADLAPDLVRSTKDVWPRFVKNRSERFEARLVTLRIEPSPSLFF
                     KDMVGSRIPVASAHGEGRAEFASPAQLELLESRSLVSARFVDGHGDTTELYPANPNGS
                     PNGIAAITTSDGRITVIMPHPERVFRTVQMSWHPPEWGRCDDSPWMRMFRNARVWVG"
     gene            complement(2913..4298)
                     /locus_tag="VFV99_27945"
     CDS             complement(2913..4298)
                     /locus_tag="VFV99_27945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063253.1"
                     /translation="MKLLYLACLLPLAGCGDDDCCKVDPDASTGGDGSNSDGRTEVAR
                     VPVTVNRDIDILFVIDDSPSMLDKQTNLKNNFPNFINVLNTVEGGLPNVHLGVVSTDL
                     GTKGADDASAGPGIGSGPGSCSGNGKAGNLQTNGSTLVVGSFISDTKNTDGTRTTNYS
                     GSLASAFSAIASLGAAGCGFEQPIEAAKRALNNNPANAGFLRPTAGLAIVMLTDEDDC
                     SISHSTLLSSDTATLGPLQSFRCTRFGVTCDNGGANSDAMNVMGSKTACHSNEQSAYL
                     KDIGSYATFFQGLKANPNAIVMAAIAAPPTPFDVELRAPPGGGTMIPALAHSCTYQGA
                     AGPEVGDPAVRVVEFLNHFPRHTFGTVCQADLSGPLIQTAQVLNGLVGSPCVYETLAM
                     PLDCVVTEEIGASKTTLPQCNNGSSSTNKPCWELLEDPASCAGGSHLKLTIERSQAPD
                     PSAVVVMRCKV"
     gene            complement(4321..5673)
                     /locus_tag="VFV99_27950"
     CDS             complement(4321..5673)
                     /locus_tag="VFV99_27950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063254.1"
                     /translation="MRFAVVLTVVLALFAACADDDSGSLADAGVTREQVALVPATARR
                     DLDLLFVTDDSPGGLELQTSLKAAFPAFLGELTLREQLPNLHIGVASTDIGAQGAEDT
                     QPGASIGSGPGSCSGVGKAGALQTNSTTLLNGTFIVDMLASDGSRTTNYTGTLTNVFN
                     AIISLGASGCGFEQPIEAMRRALDNNPANAGFQRPATPLAVITLTDEDDCSFAHSTLL
                     GGATTVLGPLQSFRCTRFGVLCDTGGATTDEMYVLGSKTRCHWNNDSAYLTRRDRYEQ
                     FIAAVNGDARNVFYGAIAGGPVSELVVEARTPPGGGSAVPALQHVCLYTGMYGPQAAD
                     PAVRIQELTEVVPRGRFEPVCDVDLTVSSVAMAREIRGLLGDPCLTRDIASPADCVVA
                     DQTLSGETPIPPCSTQRTTDCYQLVDDATCTTVSHLRVDVTRSSPASADTMVSVRCRI
                     "
     gene            complement(5714..6187)
                     /locus_tag="VFV99_27955"
     CDS             complement(5714..6187)
                     /locus_tag="VFV99_27955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063255.1"
                     /translation="MADAVETPPQISRRQIARSRRRRDDFWIGSSALLAALAALPLAQ
                     SSWHGPEVASTLAVAAVALLAGQRWAVAVIFIAELLLLPTIWPRAFFGGVELWPGRIA
                     ALASLIMIVPGLLSGRRAAAAMVLVTGWRRTRLTCRRVQYAFAVLGVIAALLPLF"
     gene            6429..6821
                     /locus_tag="VFV99_27960"
     CDS             6429..6821
                     /locus_tag="VFV99_27960"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5063256.1"
                     /translation="MSPAVVLLIKLGIRLVVFTGVFWLAAKKNDKVIFEKKWATPLVA
                     LTFAVLNTALYWVLSKLVNLATLGAVSFLMPLVINGLLLGITVRVFQSKKHWFRIDGW
                     LAMMWMAIALTAAHGLLWLGVDYIPKHI"
     gene            complement(6823..7191)
                     /locus_tag="VFV99_27965"
     CDS             complement(6823..7191)
                     /locus_tag="VFV99_27965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063257.1"
                     /translation="MRRLLLIAFVGCGDGSSSTVDAPRTDPAVCKANLESSIDRTCTM
                     ASDCVLVASADCCGTIMIAVHAGTQGMFPTVEQTYEACLACPPLGCNHADQAEDGSVP
                     QSGQAIVATCTANRCVSVVQ"
     gene            complement(7193..8113)
                     /locus_tag="VFV99_27970"
     CDS             complement(7193..8113)
                     /locus_tag="VFV99_27970"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5063258.1"
                     /translation="MPRLLWRDAQGIEGSLDLTSAEIKVGRAMDCAIRTDDAMVSRHH
                     SRIFWGGGGYVLEDLSSANGVYFQEQRVQSHLFKHGDAVRCGSLWLRYVDTAQLGGSG
                     PQSSPQPMMQHPGMGATASAGPTGHVAAINPSAAMPAQQPGAAMAALGVSAPQTNESD
                     VEIRVLRRRVEQLQTELRIYRSQKFKEDNVRRMEDLENDLSMMEIERDNLKMRLDKVE
                     SSLKVEAGSVKVKRALEIAAMTAEAAASLNDLLSSLRIEVMAAEGEFEQFAHNIPRSS
                     FELIRQSLKDAATHCDEAREMLRKLREVAD"
BASE COUNT         1345 a         2710 c         2752 g         1422 t
ORIGIN      
        1 gagggtgcga cgggccgcgg cgcgaagccg aaggcaggtc ttgtcggctt cacggtctcc
       61 gacttgcgta tccccgacgc gctcgagccg tgggagcgcg atgaatggat cggctcgccg
      121 gcgcgcatcg cgaccgcgct cgacataatg accgagggcc cgctcggcgg cgccgcgttc
      181 aacaacgagt tcggtcgccc cgcgatcctc ggctacttcc gcacgttcga gctcggcgac
      241 cgcggctatc acaagccggt gatgcttgcc ggtggcgtcg gcacggtgcg cgcgcagcac
      301 gtgcagaagg cgaaggtgcc ggcaggcgcc gcactggtcg tgctcggtgg tccggcattc
      361 ttgatcggcc tcggtggcgg cgctgcgagc tcgctcgcgc agggcgcatc tgccgaggat
      421 ctcgactttg cgtcggtgca gcgcgacaac gcggagatcc agcggcgctg ccaagaagtg
      481 atcgatcgct gctgggcgct cggcgacaag aacccgatcc tgtcgatcca cgacgtcggc
      541 gcgggcggtc tgtcgaacgc gatgcccgag ctggtgcacg acgccggcct gggcgcgaag
      601 ctcgagctgc gcaagattcc aaccggcgag cccgacctgt cgccgctcga gctgtggtcg
      661 aacgaagcgc aggagcgcta cgtgatggcg atcgggcaag agcgactcgc cgagttcgca
      721 gcgttatgcg ctcgcgagcg cgcaccgtgg gcgcagctcg gtatcgcaac gcccgacggc
      781 cggttgacgc tcgccgacga gcgcggtggc ccgccgccgg tcgatctccc actcgaggtg
      841 gtgctcggca agccgccacg catgacgcgt cgtgcggagc cgctgccgtc gccgcgccgt
      901 gcgctcgatc tcgccggtgc cagcgttgcc gacgcgctcg agcgcgtgct cggcttgccg
      961 acggtcgcgg acaagacgtt cttgatctcg atcggtgatc gcaccgtcgg tggcatggtc
     1021 agccgcgatc cgatgatcgg ccgctttcag gtgccggtcg cggattgcgc gctcaccacc
     1081 gcgggcttcg atacgtacgc gggcgaggtg atggcgcttg gcgagcggcc gcccgtcgcg
     1141 ttgctcgatg ccgcggccgc gtcgcgtctc gcgatcgcag aagcgatcac gaatctcgcc
     1201 ggtgcaccga tcggcaccgc gccgagcgcg ctcggccgca tcaagctctc gtgcaactgg
     1261 atggccgccg ccggtcaccc cggcgaggac gcgcggctat acgccggtgt gaaggcagcc
     1321 gccgagaccg cggtcgcgct cggcattgcg attcccgtcg gcaaggactc gatgtcgatg
     1381 cgcacgcagt gggacggcaa ggcagtcgtc tcgccggtca cgctcgtcgt caccgcgttc
     1441 ggtcccgtcg aggacgtgcg caagaacatc actcccgagc tacgcggtac cggcgagctg
     1501 ttgctcgtcg atctgggcgc ggggcagggc aggctcggtg cgagctgtct cgcccaggtg
     1561 ttcggtcagc tcggcgatgt gccgcccgac ctcgacgatc cgacaaagct gcgtgggttt
     1621 ttcgcgacga tgcaatcgct cgtcgcggac ggcaagttgc tcgcgtatca cgaccgcgcc
     1681 gatggcggtg cgatcgtcac cgcgctcgag atggcgttcg ccagtggcct tggcctcgag
     1741 ctcgacctga cggcgctcca tgccgatgcg ttccgcgcgc tgttcgccga ggagctcggc
     1801 gcgatcatcg aagttacgcg cgagcatgtc gatgccgtgc gggccgcgtt cgcgaagcac
     1861 aacatcggtg tccacgcgat cggccgcgcg gtcgccggtg agcagatccg cgtctcgcac
     1921 ggcggagcac tggtgctcga tgcgtcgcgc agcgagctcc gcgcgcgctg gtcgcacgtc
     1981 acgcaccaga tcgcccgccg gcgcgacgac agtgcgtgcg ccgacgaaga gcacgccacg
     2041 cgcatcgatg ccgcgaaccc cggcatcgcg accaagctca cattcgatcc cgcgcaggac
     2101 atcgccgcac cgttcatcgc gaagggtgct cgcccgcgcg tcgcgatcct gcgcgagcag
     2161 ggcgtcaacg gccagatcga gatggcggct gcgttcacgc gcgcgggctt cgatgccgtc
     2221 gatatccaca tgacggatct gatcgaaggc cgcgccgacc tcagcgactg tcgcggcgcg
     2281 atcgcgtgcg gcggcttctc gttcggcgac gtgctcggtg ccggtcgtgg ctgggcgacg
     2341 acgttccgtt acaacgcgcg cgcgcagtcc gcgatccgcc ggttcgccga tcgccacgac
     2401 acgttcgtgc tcggcgtctg caacggttgc cagatggtcg cggacctcgc gcccgacctc
     2461 gtcagatcga cgaaggacgt gtggccgcgc ttcgtgaaga accgcagcga gcggttcgag
     2521 gcgcgtctcg tcacgctgcg catcgagccg tcgccgtcgt tgttcttcaa ggacatggtt
     2581 ggctcgcgca ttcccgtcgc gagcgcgcac ggtgaaggtc gcgccgaatt cgcgagcccc
     2641 gcacagctcg agctactcga gtcacgctcg ctggtcagcg cgcgcttcgt cgatggccac
     2701 ggcgacacga cagagctgta cccggcgaac ccgaacggct cgccgaatgg catcgcggcg
     2761 atcaccacgt ccgacggccg catcaccgtg atcatgccgc atcccgagcg cgtgttccgc
     2821 accgtgcaga tgtcctggca cccacccgag tgggggcggt gtgacgacag cccgtggatg
     2881 cggatgttcc gtaacgcgcg cgtctgggtc ggctagacct tgcagcgcat cacgacgacc
     2941 gcgctcggat cgggcgcctg gctgcgctcg atggtgagct tcaagtgtga gcccccggcg
     3001 cacgaggccg gatcctcgag cagctcccag catggcttgt tcgtcgagct cgagccgttg
     3061 ttgcactgag gcaatgtcgt cttcgaggcg ccgatctcct cggtgacgac gcaatcaagt
     3121 ggcatggcga gcgtctcgta gacgcatggc gatccgacga gcccgttgag cacttgcgcg
     3181 gtttggatca acggccccga taggtctgcc tggcacaccg tcccgaacgt atggcgcggg
     3241 aagtgattca ggaactcgac cacgcgtacg gcggggtcgc cgacctcagg cccagcagcg
     3301 ccctggtagg tgcaggaatg ggcgagcgcg ggaatcatcg tgccgccacc cggcggcgcg
     3361 cgaagctcga cgtcaaatgg cgtgggcggg gcggcgatcg cggccatcac gatcgcgttc
     3421 ggattcgcct tgaggccctg gaagaacgtc gcgtagctgc cgatgtcctt caggtacgcc
     3481 gactgctcgt tcgagtgaca tgccgtcttc gagcccatca cgttcatcgc gtcagagttc
     3541 gcgccgccgt tatcgcaggt aacgccgaag cgggtgcagc ggaacgactg cagtggtccg
     3601 agcgtcgccg tgtcggagct gagcagcgtc gagtgcgata tcgaacagtc gtcctcgtca
     3661 gtcaacatga cgatcgcgag gcctgcagtc gggcggagga agccggcgtt cgccgggttg
     3721 ttgttgagcg cgcgcttcgc ggcttcgatc ggttgctcga atccgcagcc ggcggcgccg
     3781 agcgacgcga tcgccgagaa cgcgcttgcg agcgagccgc tgtagttcgt cgtccgcgtg
     3841 ccgtcggtgt tcttggtgtc actgatgaac gagccgacga cgagcgtgga accgttcgtc
     3901 tggaggttgc ccgccttgcc gttccccgag cacgagccgg gtcccgagcc gatgcctggg
     3961 cctgcactcg cgtcgtcggc gcccttcgtg ccgaggtcgg tcgatacgac accgaggtgg
     4021 acgttcggca gtccgccttc gaccgtgttg agcacgttga tgaagttcgg gaagttgttc
     4081 ttgaggttcg tctgcttgtc gagcatcgac ggcgagtcgt cgatgacgaa caggatgtcg
     4141 atgtcgcgat tgaccgtcac cggcacgcgc gcgacctcgg tgcggccgtc gctgttgctg
     4201 ccatcgccgc cggtcgatgc gtctggatcg accttgcagc agtcgtcgtc tccacagccg
     4261 gcgagcggca gcaggcaagc taggtacaag agcttcatca ggcctccaag gaggatcgta
     4321 ctagatccga caacgaaccg agaccatcgt gtcggcagaa gccggcgatg atcgagtgac
     4381 atccacacgc aggtgggaca ccgtcgtgca cgttgcatcg tcgacgagct ggtagcagtc
     4441 ggtcgttcgt tgcgtagagc agggtgggat cggtgtttca cccgaaaggg tctggtcggc
     4501 gacgacgcag tccgcgggcg acgcgatgtc gcgcgtgaga cacgggtcgc cgagcaggcc
     4561 ccggatctcg cgagccatgg cgacactcga cacagtcaga tccacgtcgc acaccggttc
     4621 gaagcgcccg cgtggcacca cctcggtcag ctcttggatg cggacggcag gatctgcggc
     4681 ctgcgggccg tacattccgg tgtagaggca cacgtgctgc agcgccggaa ccgcacttcc
     4741 gccgcctggc ggcgttcgtg cctccacgac aagctcgctg acgggtccgc ctgcgatcgc
     4801 gccatagaac acgttacgtg cgtcgccatt caccgctgcg atgaactgtt cgtatcgatc
     4861 gcggcgcgtc aggtatgccg agtcgttgtt ccagtggcag cgcgtcttcg agccgagcac
     4921 gtacatctcg tcggtcgttg cgccgccggt atcgcagagc acgccgaacc gcgtgcagcg
     4981 gaacgattgc agtggcccga gcacggtcgt ggcaccgccg agcagcgtcg aatgtgcgaa
     5041 cgagcaatcg tcttcgtcgg tgagcgtgat gaccgcgagc ggtgtcgccg gacgttgaaa
     5101 cccggcgttc gccggattgt tgtcgagggc gcggcgcatc gcttcgatcg gttgctcgaa
     5161 gccgcagccc gacgcgccga gcgagataat cgcgttgaag acgttggtca acgtgcccgt
     5221 gtagttcgtc gttcgcgacc cgtcgctcgc gagcatgtct acgatgaacg tgccgttcaa
     5281 cagcgtcgtg ctgttcgttt gcagcgcacc cgccttccca acaccagagc aggagcccgg
     5341 gcccgagccg atgctcgcgc ccggctgcgt atcctcggcg ccttgcgcac cgatgtccgt
     5401 cgacgcgacg ccgatgtgga gattcggtag ttgctcgcgc agcgtgagct cgccgagaaa
     5461 tgccgggaac gcggccttca ggctcgtctg gagctcgagc ccgcccggag aatcgtcggt
     5521 gacaaacaag aggtcgaggt cacggcgtgc ggtcgcgggc acgagcgcca cttgctcgcg
     5581 cgtcacacct gcgtcagcca gcgagccact gtcgtcgtcg gcacaggcgg cgaacagcgc
     5641 gaggacgacc gtgaggacga cggcaaaccg cacgtagcct tagtgtcggc aacgcggcgg
     5701 atccgtcaag gaatcagaac agcggaagca gtgcggcgat cacgccgagc accgcgaacg
     5761 cgtactgcac gcgtcgacat gtcagccgcg tgcgccgcca gccggtgacg agcaccatcg
     5821 cagcagcagc gcggcggccc gacaagagtc cgggcacgat catgatgagt gatgcgagtg
     5881 ccgcgatacg gccgggccac agctcgacgc cgccgaagaa cgcacgcggc cagatcgtcg
     5941 gcaagagcag cagctccgcg atgaagatca ccgcaacggc ccagcgctga ccggcgagca
     6001 acgcgactgc agcaaccgcg agcgtcgatg cgacctcggg cccgtgccag ctcgattgcg
     6061 cgagcggcaa tgcggcaagc gctgcgagta gcgccgacga gccaatccag aagtcatccc
     6121 tccgcctacg agagcgggcg atctgacgtc ggctgatctg gggtggcgtt tctactgcat
     6181 ccgccatagt ggaaatgtaa cgcatctctg acagatgtcc actgattgtc actggatgag
     6241 tacaattgta ctcgtccaag tcttcaagat tgctccgtaa aactctcgga accaacctga
     6301 taccaacacg cccgttaccg acctacgtca tcacaactcg cgcgcatccg ctactccgga
     6361 caaggcggga aaatggggtc agaccccatt tcgcagcgcg gctttgtcgc acatgacatg
     6421 ctcgacgcat gtctccggcc gtcgtgcttc tgatcaagct gggtattcgg ctcgtcgtct
     6481 tcacgggcgt gttctggctc gctgccaaga agaacgacaa ggtcatcttc gagaagaagt
     6541 gggcgacgcc gctcgtcgcg ctcacgttcg cggtgctcaa caccgcgctc tactgggtgc
     6601 tgtcgaagct cgtcaacctc gccacgctcg gcgcggtcag cttcctgatg ccgctcgtga
     6661 tcaacggcct gttactcggc atcaccgtgc gcgtgttcca gagcaagaag cactggttcc
     6721 gcatcgatgg ctggctcgcg atgatgtgga tggcgatcgc gctgaccgcc gcgcacggcc
     6781 tgttgtggct gggcgtcgac tacatcccga agcacatctg attcactgca cgacgctgac
     6841 gcagcgattc gccgtgcacg tcgcgacgat cgcctggccc gactgcggca cgctgccgtc
     6901 ctcggcctga tcggcgtgat tgcacccgag cggagggcag gcgaggcatg cctcgtacgt
     6961 ctgctcgacg gtgggaaaca tcccctgggt gcccgcatgc accgcgatca tgatcgtgcc
     7021 gcagcaatcc gccgacgcga cgagcacgca gtcgctcgcc atcgtgcagg tgcgatcgat
     7081 cgacgactcg aggttcgctt tgcacaccgc cggatccgtt cgcggcgcgt cgaccgtcga
     7141 cgatgagcca tcgccgcaac cgacgaatgc gatcagcagc aggcgccgca tgctagtccg
     7201 ccacttcgcg gagcttgcgc agcatctcgc gcgcctcgtc gcagtgcgtg gcggcatcct
     7261 tcagcgactg gcgaatcagc tcgaagctcg aacgggggat gttgtgcgcg aattgctcga
     7321 actcgccctc ggcggccatc acttcgatgc gcaggctcga cagcaggtcg ttgagcgacg
     7381 ctgccgcctc ggcggtcatc gccgcgatct cgagggcgcg cttcaccttg acactgccgg
     7441 cctcgacctt gagcgacgac tcgaccttgt cgaggcgcat cttcaagttg tcgcgctcga
     7501 tctccatcat ggacagatcg ttttcgagat cctccatgcg gcggacgttg tcttctttga
     7561 acttctggct gcgatagatg cgcagctcgg tctgcagctg ctcgacgcga cggcggagca
     7621 cgcgaatctc gacgtccgat tcgttcgtct gcggcgccga gacgccgagc gctgccatcg
     7681 cggctcccgg ctgctgcgct ggcatcgccg cgctcggatt gatcgcggcc acgtggccgg
     7741 tcggacccgc ggaggcggtc gcgcccatcc ctggatgctg catcatcggt tgcggactgc
     7801 tctgtggtcc ggagccgccc agctgcgcgg tatcgacgta gcggagccag agactgccgc
     7861 agcggaccgc gtcgccgtgc ttgaacaggt ggctctgcac ccgttgctcc tggaagtaga
     7921 cgccgttcgc gctcgataga tcctcgagca cgtagccgcc gcctccccaa aagattctgg
     7981 agtgatggcg cgagaccatc gcgtcatccg tgcggatggc gcagtccatc gcacgcccaa
     8041 ccttgatctc tgccgaggtc aggtcgagcg agccttcgat gccctgcgca tcgcgccaga
     8101 gaagtcgtgg cacgccccga aaaggtacgt gaacccgcga agctgtacaa gttctgctgg
     8161 tccttgggat cggcgccgcc gagcaatgtt ttgctcccag ctatcagccc cgcatggtaa
     8221 gtgagcgcc
//
LOCUS       DASUFX010000693         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1916079, whole genome shotgun sequence.
ACCESSION   DASUFX010000693 DASUFX010000000
VERSION     DASUFX010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916079"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFV99_27975"
     CDS             <1..861
                     /locus_tag="VFV99_27975"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5063259.1"
                     /translation="RLIDGPLCCRVEYPASPKDNVDNIAGSHRVASYHIGYAVAVPTA
                     GIVIIGDEILSGKFVEENAAFLIGELRVLGVDLRRITFVPDDLEDIATTVVATSGRFD
                     HVFTSGGVGPTHDDVTMAAIAQGFGTTVTRHPDLEARVRAYWKDKLADANLRLADIPA
                     GAELVYGKDQIWPVVCYRNVYIMPGVPTLFRRKFVDIRDRFRAEPVTAARIYVDLDEG
                     ELAPYLDAIVARYPAVRVGSYPRFSEKDFKVLVTLEGSDGTQVSGAFEALALSVGPKV
                     VRREEPASVG"
     gene            complement(866..2197)
                     /gene="hisS"
                     /locus_tag="VFV99_27980"
     CDS             complement(866..2197)
                     /gene="hisS"
                     /locus_tag="VFV99_27980"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5063260.1"
                     /translation="MSVQPRTIRGVLELLPAQQIAHQRLLDTIRANFERFGFVPIETP
                     VFELTDILLTKSGGETEKQVYFVQSTGALGQGDKPELALRFDLTVPLARYVAEHEHEL
                     NFPFRRYQMQRVYRGERAQKGRFREFYQCDIDVIGKDTLPLGYDAEVPAVIYSIFSQL
                     NFGAFTIYINNRKLLRGLLESLGIAVEKHAAVLNQVDRLGKQERAELEAKVAELTSPE
                     IAKQVFDILIANSLDALRAFEPKNANFREGLHELEDVYTNTIALGVPATALKINLSIA
                     RGLDYYTGTVYETFLNDHPNLGSICSGGRYADLASYYTKSKLPGVGISIGVTRLVSQL
                     LELGIIKADVKTTAHALVLNVEPVLGPEYRAIATELRAAGINTEVYGSDDKLGKQMKY
                     ADRSGVPVAILYGSRERDAGQVKLKILRENREIDVPRDALVASIKALLASG"
BASE COUNT          394 a          776 c          757 g          426 t
ORIGIN      
        1 cgcttgatcg acgggccgct gtgctgccgg gtcgaatacc cggcctcccc gaaggacaat
       61 gtcgacaaca tcgccggcag ccatcgggtc gcgtcttacc acatcggcta tgctgtcgcg
      121 gtgcccactg ccgggatcgt gatcatcgga gacgagatcc taagtggcaa attcgtcgag
      181 gaaaacgcgg cttttctgat cggtgaatta cgggtcctcg gcgtcgacct ccgccggatc
      241 acgttcgttc cggatgacct cgaagacatc gcgacaaccg tcgtcgcgac cagcggccgc
      301 ttcgaccacg tgttcacctc cggcggcgtc gggcccacgc acgacgacgt cacgatggct
      361 gcgatcgccc agggcttcgg caccaccgtg acgcgccatc cggatctcga ggcccgggtc
      421 cgcgcctact ggaaggacaa gctcgccgac gccaacctgc gcctcgccga catcccggcc
      481 ggcgccgagc tcgtctacgg caaggaccag atctggccgg tcgtctgcta ccgcaacgtc
      541 tacatcatgc ccggcgtccc gacgctgttc cggcgcaagt tcgtcgacat tcgcgatcgc
      601 ttccgcgccg agccggtgac cgccgcgcgg atctacgtcg acctcgacga gggcgagctc
      661 gcgccgtacc tcgacgccat cgtcgcgcgg tacccggcgg tccgcgtcgg cagctacccg
      721 cggttctccg agaaagactt caaggtgctc gtcacgctcg aaggtagcga cggcacccag
      781 gtcagcggcg cgttcgaagc actcgcgctc tcggttggcc cgaaggtcgt gcgccgcgaa
      841 gagcccgcca gcgtcggata ggttgctagc ccgacgcgag caacgccttg atgctcgcga
      901 cgagggcgtc acgcggaaca tcgatctcgc ggttctcgcg caagatcttc agcttcacct
      961 ggccggcgtc gcgctcgcga gagccgtaca ggatcgcgac cggcacaccc gagcgatcgg
     1021 cgtacttcat ctgcttgccg agcttgtcgt cgctgccgta gacctcggtg ttgatgccgg
     1081 cggcgcgcag ctccgtcgcg atcgcacggt actcggggcc gagcaccggc tcgacgttga
     1141 gcacgagcgc gtgcgcggtc gtcttgacgt cggccttgat gatgccgagc tcgagcagct
     1201 gcgagaccag tcgggtcacg ccgatcgaga tgccgacgcc gggcagcttg ctcttcgtgt
     1261 aatagctcgc gagatccgcg tagcgaccgc ccgagcagat cgagccgagg ttcgggtggt
     1321 cgttcaagaa cgtctcgtag acggtgcccg tgtagtagtc gagaccgcgc gcgatcgaca
     1381 gattgatctt gagcgcagtc gccggaacgc ccagcgcgat cgtgttcgtg tagacgtcct
     1441 cgagctcgtg caggccttcg cggaagttcg cgttcttcgg ttcgaacgcg cgcagcgcat
     1501 cgagcgagtt cgcgatcaag atgtcgaaca cctgcttcgc gatctcgggg ctggtgagct
     1561 cggcgacctt cgcttcgagc tcggcacgct cctgtttgcc gagtcgatcg acctgattca
     1621 gcacggcagc gtgcttctcg acggcaatac cgagcgattc gaggagaccg cgcagcagct
     1681 tgcggttatt tatgtaaatc gtgaaggcgc cgaagtttag ctggctgaag atgctgtaaa
     1741 tgaccgccgg cacttcggcg tcgtatccga gcggcagcgt gtccttgccg atcacgtcga
     1801 tgtcgcactg atagaactcg cggaagcggc ccttctgcgc gcgctcgccg cgatagacgc
     1861 gctgcatctg atagcgccgg aacgggaagt tcagctcgtg ctcgtgctcg gcgacatagc
     1921 gcgcgagtgg cacggtcaga tcgaagcgga gcgccaattc gggcttgtcg ccctggccga
     1981 gcgcgccggt cgactgcacg aagtacacct gcttctcggt ctcgccgccc gacttggtca
     2041 gcaagatgtc cgtcagctcg aagaccgggg tctcgatcgg cacgaagccg aaccgctcga
     2101 agttcgcgcg gatcgtatcg agcaggcgct gatgcgcgat ctgctgcgcg ggcaacagct
     2161 cgaggacgcc tctgatggta cggggctgga cggacatcga aggcggcttt ataccgcctg
     2221 aatgttcgat tgtcgccatc ggccgctacg cctgcccgac cccgtcgtcg cggagctggc
     2281 tgccgcctat ggcgaaccgc accgcgcgta ccacaacgcg gctcacatcg ccgaggtgct
     2341 cggctggttc gat
//
LOCUS       DASUFX010000694        14817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2056269, whole genome shotgun sequence.
ACCESSION   DASUFX010000694 DASUFX010000000
VERSION     DASUFX010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14817
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056269"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFV99_27985"
     CDS             <1..1245
                     /locus_tag="VFV99_27985"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX5063261.1"
                     /translation="VESVAGPLGERVQVVRDLMTLLRELGVIEPAREAAPQRGINIVV
                     AVSGAIAASHAPILVSALQRRGHVVELALTETAQRFVSVETLAALLQREPHTSMWPRA
                     AHVPVPHIALARWADLVVVYPASATTVARIANGDFSDLVSAIALTTRGAVMIAPSMNI
                     DMLEAPAVQENLDRLRENDFIIVHGVPSQEVADAPGVRPTIGGAAPPASEVAALIDAL
                     RNAGYIRRRDAWQAAYEQGLVPWASDSCDSDIAAALVEHAPPPRRLLDVGCGLGQVAR
                     HAADAGYRVVATDISDAALATARDTAGTRDIVWLRDDIGATALVGPFDVIVDRATLHT
                     LSRPRAYAWARAMRKLTARGAILIIKVHREGIANVTHGWSVAALSALLPDFEVTAERA
                     ADLPGITNEKPIASTLVVLRRR"
     gene            complement(1242..2999)
                     /locus_tag="VFV99_27990"
     CDS             complement(1242..2999)
                     /locus_tag="VFV99_27990"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5063262.1"
                     /translation="MKRLPYATAVLALIWAIPACTEAEVEDGENDAFPSGKADGGIDE
                     GTPEAVGVLRLVNDPTETAASLKSGAGVTSRVANNIVKHRDGADATAGTADDDTFDTL
                     AELDAIPYVGPATLNALLERARDRGYVSSIKLDVMFSPLPAANSHNTRIAQLIRTAQH
                     NIDIAIYSYSDAGIAAALKDRADHGVKVRFVFDTAAEDRKLTDPTARANSKSGRIEAQ
                     GIDVRWVNKTMHHKFVLIDGPRDVRENVASAKLVMGSANWSAGAATDFDENTLFIEGS
                     PELMAAFQHEFDTLWLGSRDFVGPAQSQGLSTVKILPTEVPDDAGIEPLFTSHNFTAG
                     GPDGTTWRVDKAKKELTDAWNAAIGRATTSIHVATTHMRLRPFVEALLAKKQANPNVD
                     IKIYLDQQEYISPSGDTQQKADLDACLAAATTEVQRLNCLYNDFLFSKMLVDAGITLR
                     FKSYAYRWDYTYAFQMHSKYMVVDGKELLTGSFNASMNSEQATFESGLHVFGTQFAGL
                     VGKYEQNFASIWDTGRTELAGLRAKIMTDATFPIVFPSMALTYQEFGDLRALIRANCA
                     TVDSDEFRNNPAAHKSCAR"
     gene            3160..3630
                     /locus_tag="VFV99_27995"
     CDS             3160..3630
                     /locus_tag="VFV99_27995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063263.1"
                     /translation="MRSLVGISLACSLFAFGCASDERPEDMLPSCHEPIELTATVANT
                     TDPLIKRSCFDAAVCAARDGFGVLDDIDPNVPGPQYACAVSDLAECTPYQGPPANAPC
                     WRVAIDTTCMTTQEHLALEVVYAVAPLLGTALSGSCMGECNVLHQIGRCAPVDP"
     gene            complement(3627..4247)
                     /locus_tag="VFV99_28000"
     CDS             complement(3627..4247)
                     /locus_tag="VFV99_28000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063264.1"
                     /translation="MRSTHLLVPFLSLLPTIAFAQEAAPPPPPPAALPPPPPVHRERE
                     GLELALGLQGGKIFCEGQMGQCDGFTEAGGGNITASWFFSPTLGIALDFWAMSHSDNG
                     FTFTHYVNTVALRWRPLPIVTLTGGIGAAHASLSLDNSSLAVNSDDAFAIMGAASIDV
                     IRGRRWALALEARFGNGFYGDDDNDGMADVVGRNVGAGAHFAVFGF"
     gene            4369..5355
                     /locus_tag="VFV99_28005"
     CDS             4369..5355
                     /locus_tag="VFV99_28005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5063265.1"
                     /translation="MSRLAITGVSGLLGGNLAAEAIAAGHHVIATKRAGTKIKHLADL
                     PIEWREADLSSRDALSKAFAGADAVFHCAAAVGVMREVTREMREANVTGTVNVIDACV
                     AAGVKRLVHTSSVVAIGLSTNGVPCDETATWNFDKEGLLDAYAITKHEAEQVVRAALD
                     RLDAVIVNPTYMFGPRDARPSSGGMIIKVVQRKVPGWTDGYNNFVDVRDVARGMLAAW
                     QKGRSGQRYILGGHDMTYRVCFETIAKVAGVKPPRFRVPIPIAKVFGRVGDLIEARGK
                     EPVVNSTQIRYAYTDKFRFRSQKAANELGYTYGPLETAIADALGWFRANGMA"
     gene            5513..6370
                     /locus_tag="VFV99_28010"
     CDS             5513..6370
                     /locus_tag="VFV99_28010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063266.1"
                     /translation="MDALCELPGIDVCATATDAKTALGIVDRSHVDVIVTTNDLRGAS
                     LVTLIDDARRRGVSDIIVALTARATLIPGMVDYWKDLGARYVCDRLSAVIRVTSSIVT
                     EREHAAERRRALAHQLELAVALGHSPSLRSYSSLHAGITPVRSAPPTRMLQEVSPAAL
                     LEEALGRFGKILSPDTELILEIGDDIPRVRCTVGDIERIALHLVLDARDAIPFGGKIW
                     LFVEREGVRHVRIEVMESSGRPRTPGVGVDVTRAIVKRNAGQLHIVDHEGGTMSLQVL
                     LPAVVVAPS"
     gene            complement(6367..7488)
                     /locus_tag="VFV99_28015"
     CDS             complement(6367..7488)
                     /locus_tag="VFV99_28015"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5063267.1"
                     /translation="MSEDARVRLEPLRTELGQIDRDILALVARRQALAQRIGQIKRDA
                     GIPTRDYRQERDVVERARAAAVEHGLSPTMGEELILALIRGSLTIQEKDTVAASGEGT
                     GRRALVIGGSGHMGRWFVRYLAAQGFTVESADPSDPPVRHENITYHRDWRTLTLDHEL
                     IVIAAPMPATAEILEEMAKAPPKGVVFDVGSLKSPLRNGLAKLRAAGGQVTSVHPMFG
                     PDTELLSGRHVIFIDLGVPAANAAAKALFEPTMATLVEMDLESHDRLIAYVLGLSHAL
                     NIAFFTALAESGEAAPKLANLSSTTFDAQLGVAEKVAAENPDLYFEIQTLNDYGTESL
                     AALLYAVERLRSVVRANDLDGFRALMTRGKSYLTARERR"
     gene            complement(7514..8116)
                     /locus_tag="VFV99_28020"
     CDS             complement(7514..8116)
                     /locus_tag="VFV99_28020"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX5063268.1"
                     /translation="MLARLNEETRVHHADADSDIDRYLFRAHVTVDDYRTYLARIYGF
                     LVPLEASLATSPELDGLLDVRERAKAPLVVHDLMALGMTLDEVNQLRQCGSVPAFRGA
                     ASALGWMYVIERPLLASAVLRSHLATYLRAEMAYAASYLSCYAGQVGTKWRELGEAMD
                     RLTATSAEAGVRIVLAAHEAFRALNRYRLHELVPAIRIAV"
     gene            complement(8278..9297)
                     /locus_tag="VFV99_28025"
     CDS             complement(8278..9297)
                     /locus_tag="VFV99_28025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063269.1"
                     /translation="MLSRLDRLAYHSQHLSFLINATVMEEATRLITRRPRPTIDNAVW
                     KCLQHRRSELHRRDLKNVDAGHYPRELLFDFPVRRFIREFPRLVRDAPRVVRRMRAKD
                     YKDIPEVDKERFPPYYRRTFHWQTDGYFSEHSATVYELGVELLFRGTADVMRRQIIPP
                     ISELVKASGGADKVRVLDVGCGTGRTLHQLAIAHPTMRLHGCDMSPAYIKMARTRLEH
                     IQELTLAVENGEALPWADATFDVVTSTYMFHELPRNARRNVVREMLRVVRPGGLVVIE
                     DSAQLSESPELEPVLEGFPAEFHEPFYSDYLGDDLGALLDEVGFDVQRTEPHLVAKVV
                     VGTRA"
     gene            complement(9298..10692)
                     /locus_tag="VFV99_28030"
     CDS             complement(9298..10692)
                     /locus_tag="VFV99_28030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5063270.1"
                     /translation="MRTALAGLLVLGLAATAHANGRDPYTSTINFQRGNDQHIIAGMT
                     FGAVTSSDGGATWNWMCERAIGYGGQYDPDYVYSSTGAIFATTFDGLKVMRNEGSLGH
                     GCGFTSTPPGMTFVSKTEGGPDGAIYYAAADMADAKIYKSTDDGMTFPTSANPGQPGD
                     WWSSLAVAPSNAQRVYVTGYRLDGMNPKVFLLYTSTNGGTSYTAMNMTGITPVSANST
                     IEVVGISPTTDTTLYVKVTFENGSSGDSLYRSTNAGQSWTKILSKNSNFGLAFVVRKD
                     GTTCVAGTRELGSWSSTNCSTAATPTWTLLTNAPHIGCLYNDSAGNVWACTQNYASPQ
                     LNLPSDGFGIMKTNDLTNWTGVLKYQDIKAPVACPGGTVQEDQCVQKYMDQQSAWCCL
                     VPQLGITSTAIDCNGALACFGNTVDGAPDAGTNLHPPDPTPCCGTSDARSSALLSLFV
                     TAVLLLRRRRPKEP"
     gene            complement(10634..11995)
                     /locus_tag="VFV99_28035"
     CDS             complement(10634..11995)
                     /locus_tag="VFV99_28035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5063271.1"
                     /translation="MVRVLLLIAVVALGASDAAAHGRDPIALHIEFRHDHPQDVLAGT
                     TIGLLTSHDGGATWRWTCEEAVHYQDPFDPDYAYAADGTIFAQTFTGLGIDRQTCGFS
                     PTNLGKLVISSVTTTGTGVVYAASSDATDHSIYKSIDNGVSFAPVSAPGQLGDWWRSL
                     EVAKSDSERVYLTGYRFIGTTKQFLFFVSRDGGATFDPIDTASFATTDSSMIDVVHIG
                     ANPDLVYARVTFSREPDNGDVIYRSTDAGATWGKLFTNGDPYGVSFLARSTGELVVAT
                     RLSGAWHSFDDGATWQPLPNPPHISSLVETPSGDVWAGTQNYLGIQPGGRPPIPSDGY
                     AIMKSSDLVTWTPVMRMQDLAGVACREGTDVFVQCAGTDRGLGTAWCCLVSTLGITAS
                     EIDCSGPRSCGAVAGDITVPPPDGCCQSGSQPSLLVVLLLCGYAYRPRRAACLRARGH
                     RSR"
     gene            complement(12057..12992)
                     /locus_tag="VFV99_28040"
     CDS             complement(12057..12992)
                     /locus_tag="VFV99_28040"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX5063272.1"
                     /translation="MTSQNIPVVDLGDWHEGGATRQRFVTTVGESLADIGFFAVRNHG
                     VPDALMTKSYDVARTFFHLPAATKARYNDPAKRGQRGFTGMGKEHAVNAEAADLKEFW
                     QLGRPGWTHPLYGDNVAVGEVPELAPTFVDLYQRLDGLGQVLLTACAAYLGEPADRFA
                     AMAEDSDTIVRVLFYPPVGKHVPPGAVRSAAHEDINLITLLSGATAEGLELLRRDGSW
                     MPVHTGFDTIVVDSGDMLQNVTNGLYKSTTHRVVNPRDSSSERFSIPCFIHARLDVDL
                     SPLPSCIARTGGVAKYPSITAGAYLEQRLREIGLA"
     gene            complement(12989..13246)
                     /locus_tag="VFV99_28045"
     CDS             complement(12989..13246)
                     /locus_tag="VFV99_28045"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5063273.1"
                     /translation="MAWFVYLVRCADDSLYCGVTNDLDSRVAAHGTPRGAKYTRTRGP
                     VRLVYARKCHGKRAAMRQEYRIKQLTRQAKLALVAAYTATR"
     gene            complement(13246..>14817)
                     /locus_tag="VFV99_28050"
     CDS             complement(13246..>14817)
                     /locus_tag="VFV99_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5063274.1"
                     /translation="YIRQLVTARAKAAMPDADPKTRDEVIARFVNHFHEVFEQIDRGV
                     KDLFDEAVADGEDVEKFVYAKLKLRSYEIFQSFDRDNQNELLATIDELIYADGTVHPA
                     EKKFREEIEALLDVNVVPLSDRDIEVVPTKQVEIAPEAAAPPKPKMDDHPFFQRFEHH
                     YAGDPERIRPQIQADLDLLHQFSAKLAEQRAKGNGKLTGKQSVTEFTGQAPFLDGHVW
                     VHPAQPNRRYELTVLGDLHGCYSCLKAALLQADFFAKLEAWKLDQTKPEPKLILLGDY
                     IDRGMFSYNGVLRTVMQLYLAAPEHVFPIRGNHEYYIEYRGRIYGGVKPAEAINTLIG
                     LVPGEVFQEYMKMFEELPNMLFFEDLMFVHAGIPRDADIKAKLTDMAALNDPDLRFQM
                     LWSDPSTADYIPDDLQAQNARFPFGKKQFEAFMARLGCSMMVRGHEKIDAGFKTMYGD
                     VASLLSLFSAGGAENNDLPQDSSYRTVTPMAATIVVEGGSASVTPWAIDYKRFNDPKR
                     NRFFAAPPEIEHKVG"
BASE COUNT         2498 a         4835 c         4925 g         2559 t
ORIGIN      
        1 gtcgagtcgg tcgctgggcc gctcggcgag cgcgtgcagg tggtccggga tctgatgacg
       61 ctgctccgcg agctcggcgt gatcgagcct gcgcgcgaag cggcgccaca gcggggcatc
      121 aacatcgtcg tcgcggtgag cggcgcgatc gcagcgagcc acgcgccgat tctcgtcagc
      181 gcgctgcagc gccgcggcca cgtcgtcgag ctcgcgctca ccgagaccgc gcagcgcttc
      241 gtcagcgtcg agacgctggc ggcgttgctc caacgcgagc cgcacacgtc gatgtggccg
      301 cgcgcagcgc acgtgccggt tccgcatatc gcgctcgcgc gctgggcgga cctcgtcgtc
      361 gtctaccccg cgagtgcgac gaccgtcgcg cgcatcgcaa atggcgactt ctccgatctc
      421 gtgtcggcga tcgcgctgac cacccgcggc gcggtgatga tcgcaccgtc gatgaacatc
      481 gacatgctcg aagcgccggc ggtgcaggag aacctcgaca ggctgcgcga gaacgatttc
      541 atcatcgtgc acggcgtgcc gtcgcaagaa gtcgccgacg caccgggcgt acgcccgacg
      601 atcggtggcg ccgcaccacc ggcaagcgaa gtcgccgcgc tgatcgatgc gctgcgcaat
      661 gccggctaca tccgccgccg cgatgcctgg caagccgcgt acgagcaagg gctcgtcccg
      721 tgggcgagcg acagctgcga tagtgacatc gcggcagcgc tcgtcgagca cgcgccgcca
      781 ccgcgccgcc tcctcgatgt cggctgcggg ctcggccagg ttgcgcgcca cgccgccgac
      841 gccggttacc gcgtcgtcgc caccgacatc tccgacgccg cgctcgcaac cgcgcgcgac
      901 acggccggaa cacgcgacat cgtgtggttg cgcgatgaca tcggcgcgac cgcgctcgtc
      961 ggaccattcg acgtgatcgt cgatcgcgcg acgttgcaca cgctgtcacg cccgcgcgcg
     1021 tacgcgtggg cacgggcgat gcgcaagctc actgcacgcg gcgcgatctt gatcatcaag
     1081 gtccaccgcg agggcatcgc aaacgtcacg cacggctggt ccgtcgcggc gctatccgcg
     1141 ctgctgcccg acttcgaggt cacggcagag cgcgccgcgg acctgcccgg catcacgaac
     1201 gagaagccga tcgcgtcgac gctcgtcgtg ctgcgccgcc gttagcgcgc gcaggacttg
     1261 tgggccgccg ggttgttgcg gaactcgtcg ctgtcgacgg tcgcgcagtt cgcgcgaatc
     1321 agcgcgcgca ggtcgccaaa ctcctgatac gtgagcgcca tcgacgggaa cacgatcggg
     1381 aacgtcgcgt cggtcatgat cttcgcgcgc aggcccgcaa gctcggtgcg gccggtgtcc
     1441 cagatcgacg cgaagttctg ctcgtacttt ccgacgaggc cggcgaactg cgtaccgaac
     1501 acgtgcagcc cgctctcgaa cgtcgcctgc tcgctgttca tcgacgcgtt gaagctaccg
     1561 gtgagcagct ccttgccgtc gacgaccatg tacttcgagt gcatctggaa cgcgtacgtg
     1621 tagtcccagc gatacgcgta gctcttgaag cgcagcgtga taccagcgtc gacgagcatc
     1681 ttggaaaaca agaagtcgtt gtagaggcag ttgagacgct gcacctcggt cgtcgccgcg
     1741 gcgagacagg cgtcgagatc cgccttctgc tgcgtgtcgc ccgacggcga gatgtactcc
     1801 tgctggtcga ggtagatctt gatgtcgaca ttcgggttcg cttgcttctt cgcgagcaac
     1861 gcctcgacga acgggcgcag gcgcatgtgc gtcgtcgcga cgtggatcga tgtcgtcgcg
     1921 cggccgatcg ccgcgttcca tgcgtcggtc agctccttct tcgccttgtc gacgcgccac
     1981 gtggtgccgt ccgggccgcc ggcggtgaag ttgtgcgacg tgaacagtgg ctcgatgccg
     2041 gcgtcatccg gcacctcggt cggcaggatc ttcaccgtcg acagcccctg cgactgcgcc
     2101 gggcccacga agtcgcgcga gccgagccac agcgtatcga attcgtgttg gaacgccgcc
     2161 atcagctccg ggctgccttc gatgaacagc gtgttctcgt cgaagtcggt cgccgcgccg
     2221 gccgaccagt tcgcgctgcc catcacgagc ttcgcgctcg ccacgttctc gcgcacgtcg
     2281 cgcgggccgt cgatcagcac gaacttgtgg tgcatcgtct tgttgaccca gcgcacgtcg
     2341 atgccctgcg cttcgatgcg gccactcttg ctgttcgcgc gcgcggtcgg atccgtcagc
     2401 ttgcgatcct ccgcagcggt atcgaacacg aaccgaacct tcacgccgtg gtctgcgcga
     2461 tccttcagcg cggccgcgat gccggcatcc gagtagctgt agatcgcgat gtcgatgttg
     2521 tgctgcgcgg tgcggatcag ctgcgcgatg cgcgtgttgt gtgaattcgc cgcgggcagc
     2581 ggcgagaaca tgacgtcgag cttgatgctg ctgacgtagc ccctgtcgcg cgcgcgctcg
     2641 agcaacgcgt tgagcgtcgc cgggccgacg tacggaatcg cgtcgagctc ggcgagcgtg
     2701 tcaaacgtgt cgtcgtccgc ggtgcccgcc gtcgcatcgg caccgtcgcg atgcttgacg
     2761 atgttgttcg cgacccggct cgtcacaccg gcgccgctct tcagcgacgc ggcggtctcg
     2821 gtcggatcgt tcacgaggcg cagcacgcca accgcttcgg gcgtcccttc gtcgatgcca
     2881 ccgtcggcct tgccgctcgg gaacgcatca ttttcgccgt cctcgacctc ggcctcggta
     2941 caggctggaa tcgcccagat cagagccaag actgcggtcg cataagggag gcgcttcatg
     3001 ggcgccggac atgagcaaag ccaaggccgc ttcacgtccc ccggcgaaag cactaatacc
     3061 gcgagatcat ggagtgttcg atcgccatcc ggtggcgtgt ccggctgccg gccggctgtc
     3121 cgctggccgg ccaagtagcc atgggagtag cctcggtgcg tgcggagcct cgtgggcatc
     3181 agcctcgcgt gcagtctgtt cgcctttggc tgcgcgtcgg acgagcggcc ggaagacatg
     3241 ctcccgtcct gccacgaacc gatcgagctg actgcgacgg tcgccaacac gaccgacccg
     3301 ctgatcaaac gcagctgctt cgacgccgcg gtgtgcgctg cgcgcgatgg cttcggcgtg
     3361 ctcgacgata tcgatccgaa cgtcccaggc ccgcagtacg cctgcgccgt gagcgacctc
     3421 gccgagtgca cgccctatca gggtccaccg gcaaacgcgc catgctggcg cgtcgcgatc
     3481 gacacgacat gcatgacgac gcaagagcat ctcgcgctcg aagtcgtcta cgccgtcgcg
     3541 ccgctgctcg gcaccgcgct cagcggatcg tgcatgggcg agtgcaacgt cctgcaccag
     3601 atcggtcgct gcgcgccggt cgatccctag aagccgaaga ccgcgaagtg cgcgccggcg
     3661 ccaacattgc ggccgacgac gtcggccatg ccgtcgttgt cgtcgtcgcc gtagaagccg
     3721 ttgccgaaac gcgcctcgag cgcgagggcc cagcgccggc cgcggatcac gtcgatcgac
     3781 gccgcgccca tgatcgcgaa cgcatcgtcg ctgttcaccg cgaggctcga gttgtcgagg
     3841 ctcagcgacg cgtgtgcggc accgatgccg ccggtgagcg tcacgatcgg taacgggcgc
     3901 cagcgcagtg cgacggtgtt cacgtagtgc gtgaacgtga agccgttgtc gctgtgtgac
     3961 atcgcccaga agtcgagcgc gatgccgagc gtcggcgaga agaaccagct cgcggtgatg
     4021 ttgccgccgc cggcttccgt gaaaccgtcg cactgaccca tctggccttc gcagaagatc
     4081 ttgccgccct gcagaccgag cgcgagctcg agaccttcac gctcgcggtg cacaggaggc
     4141 ggaggaggca gcgccgccgg tggagggggt ggcggcgccg cctcctgcgc aaatgcgatg
     4201 gtggggagaa gcgacagaaa tggcacgagc aagtgagtcg aacgcataca agtccttgac
     4261 cgcgacggcg gctggcaatg gtttcagcaa gctgcctgcc agcggatctg tggcgaaatc
     4321 cggcgagggt gccgcgaaag ccgacgaagc ggcataagct gccgcgtcat gagtcgcctc
     4381 gcgattacag gtgtgagcgg attactcggt ggaaatctgg cagccgaagc gatcgctgca
     4441 gggcatcacg tgatcgcgac gaagcgtgcg gggacgaaga tcaagcatct cgccgatctg
     4501 ccgatcgaat ggcgcgaagc cgatctatcg tcgcgcgatg cgctctcgaa agcattcgcc
     4561 ggcgccgacg ccgtgtttca ttgcgcggcg gcggtcggcg tgatgcgcga ggtcacgcgc
     4621 gagatgcgcg aggcaaacgt gactggcacg gtgaacgtga tcgacgcgtg cgttgccgcc
     4681 ggcgtgaagc ggctcgtgca cacgtcgagt gtcgtcgcga tcggcctgtc gacgaacggc
     4741 gtgccatgcg acgagaccgc gacgtggaac ttcgacaaag agggtctgct cgacgcgtac
     4801 gcgatcacga agcacgaggc cgagcaagtc gtgcgcgccg cgctcgatcg attggacgcc
     4861 gtcatcgtga acccgacata catgttcggc ccgcgcgacg cgcgcccgtc gtcgggcggc
     4921 atgatcatca aggtcgtgca gcgcaaagtg ccggggtgga ccgacgggta caacaacttc
     4981 gtcgacgtgc gtgacgtcgc gcgcggcatg ctcgcggcgt ggcagaaggg ccgaagtggc
     5041 cagcgctaca tcctgggcgg ccatgacatg acgtatcgcg tgtgcttcga gacgatcgcg
     5101 aaggtcgcgg gcgtcaagcc gccgcggttt cgcgtaccga ttccgatcgc gaaggtgttc
     5161 ggtcgcgtcg gcgatttgat cgaagcgcgc ggcaaggagc cggtcgtcaa ctcgacgcag
     5221 atccgttacg cgtacaccga caagttccgc ttccggtcgc agaaggcggc gaacgagctc
     5281 ggctacacgt acggcccgct cgagaccgct atcgccgacg cactcggctg gtttcgcgcc
     5341 aatggcatgg cctgattcgc gcgcgtaggc ggattcctac gcgacgttcc gcgcaccgcc
     5401 tacaacgaga tccacctgcg actgacgtcg ccgcgcagcc gcgccgcgtc tactgagcat
     5461 gatgatcagg gtgatggttg ttgctgccga agatgagcat cgggatgaga tcgtggacgc
     5521 gctttgcgag ctgccgggca tcgatgtctg cgcgacggcc acggacgcga agactgcgct
     5581 tggcatcgtc gaccgctccc acgtcgacgt catcgtcacg acgaacgatc tgcgtggcgc
     5641 ctccctcgtc acgctgatcg acgacgcgcg gcggcgtggc gtgagcgaca tcatcgtcgc
     5701 gctcaccgcg cgcgccacgc tgatccccgg aatggtcgac tactggaaag acctcggtgc
     5761 ccggtacgtg tgtgaccgac tgtcggcggt gatcagagtg acgtcgagca tcgtcaccga
     5821 acgcgagcac gcggccgaac gccgccgcgc actcgctcac cagctcgagc tggcggtcgc
     5881 cctcggtcac tcgccatcgc tgcgcagcta ctcgtcgctg cacgccggca ttacgcccgt
     5941 gcgcagcgca ccgccgaccc ggatgctcca ggaagtgtcg cccgcggcat tgctcgagga
     6001 agcgctcggc cgattcggca agatcctgtc gcccgacacc gagctgatcc tcgagatcgg
     6061 tgacgacatc ccgcgcgtgc gctgtacggt cggcgacatc gaacggatcg cgttgcatct
     6121 cgtgctcgac gcgcgcgacg cgatcccgtt cggcggcaag atctggctgt tcgtcgagcg
     6181 cgagggcgtg cggcacgttc gcatcgaggt catggagagc agcggtcggc cgcgcacacc
     6241 cggtgtcggc gtcgacgtca cgcgcgcgat cgtcaagcgt aacgcaggcc agctgcacat
     6301 cgtcgatcac gaaggcggta cgatgtcgct gcaggtcttg ctgcccgcgg tcgtggtcgc
     6361 gccgagctag cgccgctcgc gggctgtgag ataagacttg ccgcgcgtca tcagcgcgcg
     6421 gaagccatcg aggtcattcg cccgtacgac ggagcgtagc cgctcgacgg cgtacagcag
     6481 cgcagcgagc gattcggtgc cgtagtcgtt gagcgtctgg atctcgaaat agaggtcggg
     6541 gttttctgcc gcgaccttct cggcaacgcc gagctgcgcg tcgaacgtcg tcgacgacag
     6601 attcgcgagc ttcggcgcgg cttcgccact ctcggcgagc gcggtgaaga acgcgatgtt
     6661 gagcgcgtgc gacaggccga gcacgtacgc gatcaggcgg tcgtgactct cgagatccat
     6721 ttcgacgagc gtcgccatcg tcggctcgaa tagcgcctta gcggctgcat tcgcggccgg
     6781 cacaccgagg tcgatgaaga tgacgtggcg gccgctgagc agctcggtgt caggaccaaa
     6841 catcggatgg acgctcgtca cctgcccgcc cgccgcgcga agtttcgcga ggccattgcg
     6901 cagcggactc ttcagcgagc cgacatcgaa cacgacgccc ttcggcggcg ccttcgccat
     6961 ctcttcgagg atctcggcgg tcgcgggcat cggcgcggcg atcacgatca gctcgtgatc
     7021 gagcgtgagc gtgcgccagt cgcggtggta cgtgatgttc tcgtgacgca ccggcgggtc
     7081 gctcggatcc gcgctctcga ccgtgaagcc ttgcgcggcg aggtagcgca cgaaccagcg
     7141 tcccatgtga ccggagccgc cgatcacgag tgcgcggcga ccggtgccct cgccactcgc
     7201 tgcgacggtg tccttctcct gaatcgtgag cgagccgcgg atgagcgcga ggatcagctc
     7261 ctcgcccatc gtaggcgaca gaccatgctc gaccgcggcc gcgcgtgcgc gctcgacgac
     7321 atcgcgctcc tgtcgatagt cgcgggtcgg aatgccggcg tcgcgcttga tctggccgat
     7381 ccgctgcgcg agcgcttgcc gcctcgcgac gagcgcgagg atgtcgcgat cgatttgacc
     7441 gagctcggta cgaaggggct ctagtctgac ccgtgcgtct tcggacatgg gtcgctaagg
     7501 tacacccaac gggtcagact gctatgcgga tcgcgggcac gagctcgtgc aaccgatagc
     7561 gattcagcgc gcggaatgcc tcgtgggcgg ccagtacgat gcgcacgcca gcctccgccg
     7621 acgttgcagt gaggcgatcc atcgcctcgc cgagctcgcg ccacttggtg ccgacctggc
     7681 cggcatagca gctgagatac gacgccgcgt aggccatctc ggcgcgcaga tacgtcgcga
     7741 ggtggctacg caacaccgcg ctcgcgagca gcggccgttc gatcacgtac atccaaccga
     7801 gcgcgctcgc tgctccacga aacgccggca cgctgccgca ctgacggagc tggttgacct
     7861 cgtcgagcgt catgccgaga gccatcagat cgtgaacgac aagtggtgcc ttcgcgcgct
     7921 ctcgaacgtc gagcaggcca tcaagctccg gggacgtcgc gagcgaggcc tcgagcggga
     7981 ccaagaagcc gtagatccgg gccaggtagg tccggtagtc gtcgaccgtg acgtgcgccc
     8041 ggaacaggta ccgatcgatg tcactgtcgg catcagcgtg atggactcgc gtctcctcgt
     8101 tcaggcgagc cagcattcgc aggtcgtcca tcgcgcggcc tgtactgcaa aacacggacg
     8161 actcgccatg tgacgcaaat ccgcacggtt aacgcgtacg caaacgcgcg tacgagcgca
     8221 tcgagcggtt tcgccacgtg gcgtcggcgc tcagcttccc ggttccttac gggatcttta
     8281 cgcccgcgtc ccgacgacca ctttggcgac gaggtggggc tcggtgcgct gaacgtcgaa
     8341 gccgacctcg tcgagcagcg cgccgagatc gtcgccgagg taatccgaat agaacggctc
     8401 gtggaattcg gccgggaagc cctccaggac cggctcgagc tccggactct cggacagctg
     8461 cgccgagtct tcgatcacga cgagaccgcc cggccgcacc acgcgcagca tctcgcgcac
     8521 gacgttgcga cgcgcgttgc gcggcagctc gtggaacatg tacgtgctcg tcacgacgtc
     8581 gaacgtcgcg tcggcccacg gcaatgcttc gccgttttcg acagcgagcg tcagctcctg
     8641 gatgtgctcg agccgcgtgc gcgccatctt gatgtatgcc ggcgacatat cgcagccgtg
     8701 caggcgcatc gtcggatgcg cgatcgcgag ctggtgcagc gtgcgccccg tgccgcagcc
     8761 gacgtcgagc acgcgaacct tgtcggcgcc gccgctcgcc ttcacgagct cgctgatcgg
     8821 cggaatgatc tggcgccgca tcacgtctgc ggtgccgcgg aatagcagct cgacgccgag
     8881 ctcgtagacg gtcgccgaat gctcgctgaa gtagccgtcc gtttgccagt ggaacgtacg
     8941 ccggtagtac ggcgggaatc gctccttgtc gacttcggga atgtccttgt agtccttcgc
     9001 gcgcatccgg cgcacgacgc gaggcgcatc acgcaccagg cgcgggaact cgcgaatgaa
     9061 ccgccgcacc ggaaagtcga acaacagctc gcgcggatag tgaccggcgt cgacgttctt
     9121 cagatcgcgg cgatgcagct cgctgcgccg atgctgcagg cacttccaga ccgcgttgtc
     9181 gatcgtcggc cgaggcctgc gcgtgatgag cctcgtcgcc tcctccatca cggtcgcatt
     9241 gatcaagaac gacaaatgct gcgagtgata tgcgaggcgg tcgaggcgcg acagcatcta
     9301 gggctccttg ggtctcctgc gacggagtag caacaccgcc gttacaaaca agcttagcag
     9361 tgcggacgat cgcgcatcac tggttccgca acacggcgtg ggatcgggcg gatgaaggtt
     9421 cgttcccgca tcgggtgcgc catcgacggt attgccaaaa cacgcaagtg cgccattgca
     9481 gtcgatcgcc gtcgacgtga tcccgagctg cggcaccagg cagcaccacg ccgactgctg
     9541 atccatgtac ttctgaacgc actgatcttc ctgcaccgta ccgccggggc acgcgaccgg
     9601 tgccttgatg tcctgatact tgagcacgcc ggtccagttc gtcagatcgt tcgtcttcat
     9661 gatgccgaag ccgtccgacg gcaggttgag ctgcgggctc gcgtagttct gcgtgcacgc
     9721 ccagacgttg ccggcactgt cgttgtagag gcagccgatg tgcggcgcgt ttgtcagcag
     9781 cgtccaggtc ggcgtcgccg ccgtcgaaca gttcgtcgac gaccacgagc cgagctcgcg
     9841 cgtgccggcg acgcacgtcg tgccgtcctt ccgcacgacg aacgcgagac cgaagttcga
     9901 attcttcgac aggatcttcg tccagctctg gccggcgttc gtcgagcgat agagcgagtc
     9961 accgctggaa ccgttttcaa acgtcacctt gacgtacagc gtggtgtccg tcgtcggcga
    10021 gatgccgacc acttcgatcg tggagttcgc cgacaccggc gtgatgccgg tcatgttcat
    10081 cgcggtgtag ctcgtgccgc cgttggtgct cgtgtagagc aagaacacct tcgggttcat
    10141 gccgtcgagt cgatagccgg tgacgtagac gcgttgcgcg ttcgacggcg cgaccgcgag
    10201 cgacgaccac cagtcgccgg gctggccggg gttcgccgag gtcgggaacg tcatgccgtc
    10261 gtcggtcgac ttgtagattt tcgcgtcggc catatccgcg gccgcgtagt agatcgcgcc
    10321 gtctgggcca ccctcggtct tcgacacgaa cgtcatgccg ggcggcgtcg acgtgaagcc
    10381 gcagccgtgc ccgagcgagc cctcgttgcg catgactttc aggccgtcga acgtggtcgc
    10441 gaagatcgcg ccggtcgacg agtacacgta gtcgggatcg tattgaccgc cgtagccgat
    10501 cgcgcgctcg cacatccaat tccacgtcgc gccaccgtcc gacgacgtca ctgcgccgaa
    10561 cgtcatgccg gcgatgatgt gctggtcgtt gccgcgctgg aagttgatgg tcgacgtgta
    10621 cgggtcgcga ccgttagcgt gagcggtggc cgcgagccct aagacaagca gcccggcgag
    10681 ggcggtacgc atatccgcag agtaacagga ctactagcaa gctcggctgc gaaccgctct
    10741 gacaacagcc gtcaggggga ggcactgtga tgtcacccgc aacggcgccg caactacgcg
    10801 gaccggaaca gtcgatttcg gacgctgtga tcccgagcgt cgacaccaaa cagcaccacg
    10861 cggtgccgag gccgcgatcc gttcccgcac attgcacgaa cacatcggtg ccctctcgac
    10921 acgcgacgcc agcgagatcc tgcatgcgca tcaccggtgt ccacgtcacg aggtcgctcg
    10981 atttcatgat cgcgtagccg tcgctaggaa tcggcggccg cccacccggt tggatgccga
    11041 gatagttctg cgtgccggcc cagacgtcgc cactgggagt ctcgacgagc gagctgatgt
    11101 gcggcgggtt cggcagtggt tgccacgtcg cgccgtcgtc gaacgagtgc cacgcaccac
    11161 tgagccgcgt cgcgacgacg agctcgccgg tcgagcgcgc gaggaacgag acgccatatg
    11221 gatcgccgtt cgtgaacagc ttgccccacg tcgcgccggc atcggtcgag cgatagatca
    11281 cgtcgccgtt gtcgggttct cgcgagaacg tgacgcgcgc gtagacgagg tcgggattcg
    11341 cgccgatgtg gacgacatcg atcatcgagc tgtcggtggt cgcgaaactc gccgtgtcga
    11401 tcggatcgaa cgtcgcccca ccgtcgcgac tgacgaaaaa caagaactgc ttcgtggtgc
    11461 cgatgaaacg atagccggtg agatacacgc gctcggaatc actcttcgcc acctcgagcg
    11521 agcgccacca atcgccgagc tggcctggtg cgctgaccgg cgcgaacgag acgccgttgt
    11581 cgatcgactt gtagatcgag tggtcggtcg cgtctgacga ggcggcatac acgacgccgg
    11641 ttccggttgt cgtcaccgac gagatcacga gcttgccgag gttcgtcggc gaaaagccgc
    11701 aggtctggcg atcgatgccg agcccggtga acgtctgcgc gaagatggtg ccgtccgcgg
    11761 catacgcata gtcgggatcg aacggatcct ggtaatgcac tgcttcttcg cacgtccacc
    11821 gccaggtcgc gccaccgtcg tgcgacgtga gtaagccgat cgtcgtgccg gccagcacgt
    11881 cttgcggatg atcgtgacga aactcgatgt ggagcgcgat cggatcgcga ccatgggctg
    11941 ccgcatcgct cgcgccgagg gcgacgaccg cgatcagcag caggacgcga accatgcgcg
    12001 cagactctac tgcgagccgg gcgccgtcca agtgcgcctg ctcacacgcg cttgcctcat
    12061 gcgaggccga tttctcgcaa tcgctgctcg aggtaggcgc cggcggtgat gctcgggtac
    12121 ttcgccacgc cgccggtgcg cgcgatgcag ctgggcagcg gcgacagatc gacgtcgagc
    12181 cgcgcgtgga tgaagcacgg gatcgagaac cgctcgctcg acgaatcgcg cgggtttacc
    12241 acccgatgcg tcgtgctctt gtagagcccg ttcgtcacgt tctgcagcat gtcgccggag
    12301 tcgacgacga tcgtgtcgaa cccggtgtgc accggcatcc acgagccatc gcggcgcaag
    12361 agctcgaggc cttcggccgt cgcgcccgag agcagcgtga tcaagttgat gtcctcgtgc
    12421 gcggcggagc gcacggcgcc gggcgggacg tgcttgccga cgggcggata gaacaacacg
    12481 cgcacgatcg tgtcgctgtc ctccgccatc gcggcgaagc gatcagccgg ctcgccgaga
    12541 tatgcggcgc acgcggtgag cagcacctga ccgagaccat cgaggcgctg atacaagtcg
    12601 acgaacgtcg gcgcgagctc gggcacctcg ccgaccgcga cgttgtcgcc atagagcgga
    12661 tgcgtccagc ccgggcgacc gagctgccag aactccttga ggtcggcggc ctcggcgttg
    12721 accgcgtgct ccttgcccat gccggtgaaa ccgcgctggc cgcgctttgc cggatcgttg
    12781 tagcgggcct tcgtcgcagc gggcagatga aagaacgtgc gcgcgacgtc gtacgacttc
    12841 gtcatcagcg catcggggac gccgtggttg cgcactgcga agaagccgat gtcggcgagc
    12901 gattcaccga cggtggtgac gaaccgctgg cgtgttgcgc cgccctcgtg ccaatcccct
    12961 agatcaacaa ccgggatgtt ctgcgaggtc atcgcgtcgc agtatacgcg gccacgagcg
    13021 cgagcttcgc ttgacgcgtc agttgcttga tccggtattc ctggcgcatc gccgcacgtt
    13081 tgccatggca cttgcgggcg tagacgagac gcacgggtcc gcgcgtccgc gtgtacttcg
    13141 cgccgcgtgg agtgccatgg gccgcaacac ggctgtcgag atcgttcgtg acgccgcagt
    13201 acagcgagtc gtcagcgcag cgaaccaggt agacgaacca agccactagc caactttgtg
    13261 ctcgatctcg ggcggcgccg caaagaatcg gttgcgcttg ggatcgttga accgcttgta
    13321 gtcgatcgcc catggggtca ccgacgcgct gccgccctcg acgacgatcg tcgccgccat
    13381 cggcgtcacg gtgcgatagc tcgagtcttg cgggaggtcg ttgttctcgg cgccaccggc
    13441 ggagaacagc gacaacagcg acgcgacgtc gccgtacatc gtcttgaagc cggcgtcgat
    13501 cttctcgtgg ccgcgcacca tcatcgagca gccgagccgc gccatgaacg cttcgaactg
    13561 cttcttgccg aacggaaacc gcgcgttctg tgcctgcaga tcgtcgggaa tgtagtcggc
    13621 cgtcgacgga tcgctccaca gcatctggaa gcggaggtcc ggatcattga gcgcggccat
    13681 gtccgtcagc ttcgccttga tgtcggcgtc acgcggaatg ccagcgtgca cgaacatgag
    13741 gtcctcgaag aacagcatgt tcggcagctc ttcgaacatc ttcatgtact cctggaacac
    13801 ctcgccaggc acgagaccga tcagcgtgtt gatcgcttcg gccggcttca cgccgccgta
    13861 aatgcggccg cggtactcga tgtagtactc gtggttgccg cggatcggga acacgtgctc
    13921 gggcgccgcg agatacagct gcatcaccgt gcgcagcacg ccgttgtagc tgaacatgcc
    13981 gcgatcgatg taatcgccga gcaggatcag cttcggttcg ggtttggtct gatcgagctt
    14041 ccacgcttcg agcttcgcga agaagtcggc ctggagcagc gcggccttga gacagctgta
    14101 acagccgtgc aggtcgccga gaacggtcag ctcgtagcgg cggttcggct gcgcggggtg
    14161 gacccacaca tggccgtcga ggaacggtgc ctggccggta aactcggtga cggactgctt
    14221 gccggtgagc ttgccgttcc ccttcgcgcg ctgctcggcg agcttcgcgc tgaactgatg
    14281 gagcagatcg aggtcggcct ggatctgagg ccggatgcgc tcggggtcgc cagcatagtg
    14341 atgctcgaag cgctggaaga acggatggtc gtccatcttc ggtttcggcg gcgcggccgc
    14401 ctcgggagca atctcgacct gcttggtcgg gacgacctcg atgtcgcggt ccgacagcgg
    14461 cacgacgttg acgtcgagca gcgcttcgat ctcctcgcgg aacttcttct ccgccgggtg
    14521 gacggtgccg tcggcgtaga tgagctcgtc gatggtcgcc aggagctcgt tctggttatc
    14581 gcggtcgaag ctctgaaaga tctcgtagct gcggagcttg agcttcgcgt agacgaactt
    14641 ctcgacgtcc tcgccgtcgg cgaccgcctc gtcgaacagg tccttgacgc cgcgatcgat
    14701 ctgctcgaac acctcgtgga agtggttgac gaagcgcgcg atcacctcgt cgcgcgtctt
    14761 cggatcggca tcgggcatcg cggccttggc gcgcgccgtc accagctgcc ggatata
//
LOCUS       DASUFX010000695         8492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1640855, whole genome shotgun sequence.
ACCESSION   DASUFX010000695 DASUFX010000000
VERSION     DASUFX010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640855"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="dinB"
                     /locus_tag="VFV99_28055"
     CDS             <1..1051
                     /gene="dinB"
                     /locus_tag="VFV99_28055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5063275.1"
                     /translation="YEARKFGIHSAMPMAEAMRRCPHAIVVRHDMERYAEASRLFFAI
                     LGDFSPDVEGLSLDEAFLDVTGSERLLGDGRTIAEAIKKRVRAELSLVASVGVAPIKL
                     AAKIASDIDKPDGLRVVTPEGLLSFLHSLPVTRLWGVGGATREILASMGLSTIGDVAR
                     YPEAALVARLGAASGQHLAALARGDDPRPVISEHEQVTIGHQETFDEDIDDKGELGVI
                     LLDQADRVAVRLRDDQLRARAVVLIVKYDDFRQITRRTTLDAATSDGGVLARAAIELL
                     SKVQIEDRKGMRVRLCGVSAQSLEPRDAPRQLGFDEAAREKGERLGDTLDKVTAKFGK
                     SAIKRAILVTRKDEE"
     gene            1101..2744
                     /locus_tag="VFV99_28060"
     CDS             1101..2744
                     /locus_tag="VFV99_28060"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5063276.1"
                     /translation="MWKALLALACLSSLLGTAHAEEPIATYAIVVGSNTGGPGQTDLR
                     YAEDDARKVGALLVELGGYKSDQVDVVVHPTPEQLRAHINALADKLNADLAAGRPSRV
                     FFYYSGHARATALDLGSDELLLVELRQRLFQLPAKLTVVVLDACQSGAFSRVKGAQPA
                     ADFSFNSRQQLDATGVAVLASSSGSELSQESEQLKGSYFTHHLLVGMRGASDTNKDGL
                     VTVDEAYRYAYNQTLLATAETAVGGQHVSFEADLKGHGEVPLSYPRAATSSIELPATL
                     EGKTLVEDKRAHSVVAETYKAKGAAVRIAVAPGDYTVMVRQGTTLSRCEVSGGGVVDL
                     GKCSKEELVTTTTKGGGFTRPYRVEIQLVLGAERKDGYTDTLEAFGYKAKFLGATTGL
                     YVSGLRRLTKYFWVGGIVSDEATDEWTRDTDGLYPLRFKWAMTTLAAAGRAETAFAED
                     GFFSTIKVYSQLGLGLGIGRTTLHDQDDMTTKDSYLGPALSLGAGFLFGGDDHNYILK
                     GTAFSLGYQFEYAPVIENLIGDTHAVGGHRLSAALSYSF"
     gene            2752..3918
                     /locus_tag="VFV99_28065"
     CDS             2752..3918
                     /locus_tag="VFV99_28065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063277.1"
                     /translation="MRKLPTLLLFGALATQLGNTNCGQALRDPGYDLWCSDESGGSYY
                     LCAWKVERGDIKRVPTWHEGDSGVELVGTDAAIAQLSPVDSADGQCRDVDGKSECTSP
                     DNVCLEFSLLANIDDNAVVDLNMDIFNDGTIENTQRLPIGKWQTLSYKVVIGQPFAGI
                     RFELAKSGTGTAQLANIGAHLADNCDGLPVIEPGPAPLGAPCADASDCVSGICANGES
                     PVPFPGVAGFLQPVKVCVGCSPEQNCSGGTICGVGDATSPVRAQETVCVVPAAKQLGE
                     SCFWFNECASNYCTGGVCSTCKDNTQCGGGEQCGEGWDTVFSAWVCNPNGGARQSGEP
                     CVSHADCASGVCNGTERMQCEDGRECVTAAQCPFKEGLKNGPCTTVGIQGGSCQ"
     gene            3902..4390
                     /locus_tag="VFV99_28070"
     CDS             3902..4390
                     /locus_tag="VFV99_28070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5063278.1"
                     /translation="MEAVSDPQAAYRAYGPALVRKAERVLRSREDAVDVVHTLFADLI
                     PRWSKDVDLPYLYRAVTNRCLNVLRDRGTRARLLEREQQVAAPVGRVRLDDEVVGVGL
                     IAKLADRLDEGHLEVLVARFVDDMTQEEIAEHLGVSRKTIGKRLDRIRDEVIALRTEA
                     AG"
     gene            4390..5010
                     /locus_tag="VFV99_28075"
     CDS             4390..5010
                     /locus_tag="VFV99_28075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063279.1"
                     /translation="MATIACIGEPISWLRLERYALAGATEPTIRAHVEACAACRQCLD
                     EIQRDVVALPPLHVSVTKRRRWWTLALPAFGALAAAAILLLVLRPREPKQADNIVRIK
                     GVGEVIVDVVRERGGVVREDVRSYQAGDRWKVVVTCPPTHEVSLAVAVREDGAEHADR
                     PLAPAKILCGNRIYVPGAFELTGGKPHRVCVRIEDQADACLTLAPE"
     gene            complement(5020..5562)
                     /locus_tag="VFV99_28080"
     CDS             complement(5020..5562)
                     /locus_tag="VFV99_28080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063280.1"
                     /translation="MHLVEPEEVGPIEGDDQVVTPPRPPHRRVSVSLLFTLSVLIGLV
                     VTIYLVLPARHNVLVTEAIERHREPGAWDLEAPTMPELRAWTIGVVGKDVPLPADVTA
                     VIGARRVEVLNRDGALMRLKLGSDEISYIVQKGRGIAPQHNEKHDDLHATAWRCGKYN
                     CVAVGPQASSSTWLAAFKKK"
     gene            complement(5535..5873)
                     /locus_tag="VFV99_28085"
     CDS             complement(5535..5873)
                     /locus_tag="VFV99_28085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063281.1"
                     /translation="MRFCPWYPLADAAAHAPAAEGVLQVRIAAGLLDYPTGKSAMVHY
                     EYAQNVRAAATDLAEQHAGEALLCRHLEIAPGDAVDLAAFHAKVREEFVRRFGTSPSF
                     SPCISSNPKK"
     gene            5896..6801
                     /locus_tag="VFV99_28090"
     CDS             5896..6801
                     /locus_tag="VFV99_28090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5063282.1"
                     /translation="MEQIETRQRIERVEFGRDLALADRLVHGDSLEVAESLPTATLDA
                     IYIDPPFGTGTVRRGRGLRYTDRSDDPEAFVAWLMPFLEQSKRTLVEHGSLFVHLDYR
                     AVHYVKVALDQLFGRDRFINEIVWCYAVGGKSRRGFGRKHDTILWYARSADWAFYPDA
                     VRVPRRGGSHMRLVDGIQEKTDRKTGRVYRYPVHAGKVPEDWWTDIETLNHSDRERTG
                     WPSQKPERLLERLICAVTKPGERIADWFSGSGTTAAVAQRLGRRFLVVDREADAVAVC
                     ANRLLGQGRALASRGTPPPPLEILR"
     gene            6858..8201
                     /locus_tag="VFV99_28095"
     CDS             6858..8201
                     /locus_tag="VFV99_28095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5063283.1"
                     /translation="MTAEAADEATRVTLEAWLVTDVGVVRDHNEDSAFMEPSQGFFIV
                     ADGMGGHAAGEVASAMAVETVRTTLEAAKPEIEAFKQGPTDAGRRGLVQLLQSAVLQA
                     HQSVFQRGQAETDKQGMGTTLDVVLIAGGEAFVAHVGDSRTYLVREGRSSQITTDHTV
                     AEVLVIEGKLTIEEAQVSPLRTILVNAIGVSADVGVEMAHVTLKKGDRLLLCSDGLHD
                     YFPVEDEIAQRMSAEKPGEALKEMVELAKTRGGHDNITGVAVHVIDLIEAAVPKGLSE
                     DSTQPVDIQGNPFAADEPTQTAANGGNTGTPPETPTEAPPGTPTGGNFRRTEPMRAIN
                     EVGTLPTLPPPNKRKDDVKKGDKKDAAKKTDVSTDVAADDAQLDKKGKSAKDETPKPA
                     DKKPDETPKPADKPDGDPKAADPKSDAKSDESGDAVPVEAVGTADTGRVTAPKKD"
     gene            complement(8277..>8492)
                     /locus_tag="VFV99_28100"
     CDS             complement(8277..>8492)
                     /locus_tag="VFV99_28100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063284.1"
                     /translation="FVQKGKQEVFGCSDAGRVPLRLLKRNRSDANRAFERTRRGDVLV
                     VDARAGEHERVEIEADTTVERREPAGK"
BASE COUNT         1490 a         2836 c         2844 g         1322 t
ORIGIN      
        1 gtacgaggcg cgcaaattcg gcatccattc cgcaatgccg atggccgagg cgatgcggcg
       61 ctgtccgcac gcgatcgtcg tacgccacga catggagcgc tacgccgaag catcgcgcct
      121 gttcttcgcg atcctgggcg acttttcgcc ggacgtcgaa ggcctgtcgc tcgacgaagc
      181 gttcctcgat gtcaccggca gcgagcggct gctcggtgat ggccgcacga tcgccgaagc
      241 gatcaagaag cgcgtgcgcg cggagctctc actcgtcgcg tcggtcggcg tcgcgccgat
      301 caagcttgcc gcgaagatcg catccgacat cgacaagccc gatggcctcc gtgtcgtcac
      361 gcccgagggc cttctctcgt ttctgcactc gttgccggtc acgcggctat ggggcgtcgg
      421 tggcgcgacg cgcgagatcc tcgcaagcat gggtctatcg acgatcggcg acgtcgcgcg
      481 ctatcccgag gctgcgctcg tcgcccggct cggcgcggcc agtggccagc acctcgccgc
      541 actcgcgcgc ggtgacgatc cgcgtcccgt gatctcggag cacgagcaag tcaccatcgg
      601 ccatcaagag acgttcgacg aagacatcga cgacaagggc gagctcggcg tcatcttgct
      661 cgaccaggcc gatcgcgtcg cggttcgcct gcgcgacgac cagctgcgcg cccgcgccgt
      721 cgtcctcatc gtcaagtacg acgactttcg ccagatcacc cgccgcacca cgctcgatgc
      781 ggcgaccagc gatggcggcg tgctcgcccg cgccgcgatc gagctgctct cgaaggtgca
      841 gatcgaagat cgcaaaggca tgcgggtgcg gctgtgcggg gtctcggcac agagtctcga
      901 gccgcgcgat gcgccgcgcc agctcggctt cgacgaggcc gcgcgagaga aaggtgagcg
      961 gctcggcgac acgctcgaca aggtgacggc gaagttcggc aagagtgcga ttaagcgggc
     1021 gattctcgtc actcgcaaag acgaagagtg attttggggt tcccaaaggc cggaactggc
     1081 gtgtcattag ggcaaagacc atgtggaaag cgctgcttgc cctcgcgtgt ctgtcgtcgc
     1141 tgctcggcac ggcacacgcc gaggaaccga tcgcgaccta cgcgatcgtc gttggctcga
     1201 acaccggcgg accgggacaa accgatctgc gttatgcgga agacgacgcg cgcaaagtcg
     1261 gcgcgctgct cgtcgagctc ggcggctaca agtccgacca ggtcgacgtc gtcgtccacc
     1321 cgacgcccga gcagttgcgc gcgcacatca acgcgcttgc cgacaagctc aacgccgacc
     1381 tcgcggccgg ccgcccgagt cgcgtgttct tctattacag tggtcacgct cgcgcgaccg
     1441 cgctcgacct cggttccgac gagctgctgc tcgtcgagct gcgccagcga ttgttccagc
     1501 tgcccgcgaa gctgacggtc gtcgtactcg atgcctgcca gagcggtgcg ttctcgcgcg
     1561 tcaaaggcgc gcagcccgcc gccgacttct cgttcaactc gcgccagcag ctcgacgcga
     1621 ccggtgtcgc cgtgctcgcg tcgtcctcgg gcagcgagct gtcgcaggag agcgagcagc
     1681 tcaagggctc gtacttcacg catcacctac tcgtcggcat gcgcggtgcg agcgacacga
     1741 acaaggacgg gctcgtcacc gtcgacgagg cgtatcgcta cgcgtacaac cagacgctgc
     1801 tcgccaccgc ggagaccgcg gtcggcggcc agcacgtgtc gttcgaggcc gatctcaaag
     1861 gtcacggcga agtgccgctc tcgtatccgc gtgcagcgac gagcagcatc gaattgccgg
     1921 ccacactgga aggcaagacg ctcgtcgaag acaagcgcgc gcactcggtc gtcgccgaga
     1981 catacaaggc gaagggtgcg gcggtgcgga tcgcggtcgc gcctggcgat tacaccgtga
     2041 tggtgcggca aggcacgacg ctgtcgcgct gcgaggtcag cggcggtggc gtcgtcgacc
     2101 tcggcaagtg ctcgaaggag gagctcgtca ccacgacgac gaaaggcggc ggtttcacgc
     2161 ggccgtatcg cgtcgagatc cagctcgtgc tcggcgccga gcgcaaggac ggctacaccg
     2221 acacgctgga agcgttcggc tacaaggcga agtttctcgg cgcgacgacc ggcctgtacg
     2281 tcagcggtct gcgccggctc acgaagtact tctgggtcgg cggcatcgtc agcgatgaag
     2341 cgaccgacga gtggacgcgc gataccgacg gcctctatcc gctccgcttc aagtgggcga
     2401 tgacgacgct ggccgccgcc ggccgcgccg agaccgcgtt cgccgaagac ggcttcttct
     2461 cgaccatcaa ggtgtacagc cagctcgggc tcgggctcgg catcggccgc acgacgctgc
     2521 acgaccagga cgacatgacg acgaaggaca gctacctcgg gccggcactg tcgctcggcg
     2581 cgggcttcct gttcggcggc gacgaccaca actacatcct caagggcacc gcgttctcgc
     2641 tcggctatca gttcgagtac gcccccgtga tcgaaaacct gatcggcgac acgcacgcgg
     2701 tgggtggcca ccgcctgagc gccgccctct cgtacagctt ctaaggctcc catgcgcaaa
     2761 ctacccaccc tccttttgtt cggcgcgctc gcgacgcagc tcggcaatac gaactgcggc
     2821 caggcgctgc gcgatccggg ctacgacctg tggtgcagcg acgagagtgg cggaagctac
     2881 tacctgtgcg cgtggaaggt cgagcgcggt gatatcaagc gcgtgccgac gtggcacgaa
     2941 ggcgactccg gcgtcgagct cgtcggcacc gacgccgcga tcgcgcagct ttcgcccgtc
     3001 gactcggccg acggccagtg ccgcgacgtc gacggcaagt cggagtgcac gtcgcccgac
     3061 aacgtctgcc tcgagttctc gctgctcgcg aacatcgacg acaacgcggt cgtcgatctc
     3121 aacatggaca tcttcaacga cggcacgatc gagaacacgc agcggctgcc gatcggcaag
     3181 tggcagacgc tcagctacaa ggtcgtgatt ggccagccgt tcgccggcat ccgcttcgag
     3241 ctcgcgaaga gcggcaccgg cacggcgcag ctcgcaaaca tcggcgcgca cctcgcggac
     3301 aactgcgacg gcttgccggt gatcgagccc ggcccggcgc cgctcggcgc gccgtgcgcc
     3361 gatgcgagtg actgtgtcag cggcatctgc gcgaacggcg aatcgccggt gccgttcccc
     3421 ggcgtcgccg gttttctgca gccggtcaag gtgtgcgttg gctgcagccc cgagcagaac
     3481 tgctccggcg gcacgatctg cggcgtcggc gatgcgacgt cgccggtgcg cgcgcaagag
     3541 actgtctgcg tggtgcccgc cgcgaagcag ctcggcgagt cgtgcttctg gttcaacgag
     3601 tgcgcgagca actactgcac cggcggcgtc tgctcgacgt gcaaggacaa cacgcagtgt
     3661 ggcggcggcg agcaatgcgg tgaaggctgg gacaccgtgt tctcggcctg ggtgtgcaac
     3721 ccgaatggtg gcgctcgcca atcgggagaa ccgtgcgtga gtcatgccga ctgcgcgagc
     3781 ggggtctgca atggcaccga gcgcatgcaa tgcgaagacg gccgcgaatg cgtgaccgcc
     3841 gcgcaatgcc cgttcaagga gggcctcaag aacgggccct gcactacagt cggcatccaa
     3901 ggtggaagct gtcagtgatc cccaggctgc gtatcgcgcc tacggtcctg cgttagtacg
     3961 caaggccgag cgtgtgctgc gcagtcgcga ggacgctgtc gatgtcgtgc atacgttgtt
     4021 cgccgatctg attccgcgct ggagcaaaga cgtggacctg ccctacctct accgcgccgt
     4081 caccaaccgt tgcctcaacg tccttaggga ccgcggcacg cgggcgcgct tgctcgagcg
     4141 cgagcagcag gtggcagcgc cggtcgggcg cgtgcggctc gacgacgagg ttgtcggcgt
     4201 gggactgatc gcgaagctcg ccgatcgcct cgatgaaggt catctcgagg tcctcgtcgc
     4261 gcgctttgtc gacgacatga cgcaagaaga gatcgccgag cacctcggcg tgtcgcgcaa
     4321 gacgatcggc aagcggctcg atcgcattcg cgacgaggtg atcgccctgc gcacggaggc
     4381 ggccggctga tggcaacgat cgcctgcatc ggtgagccga tctcgtggct gcgcctcgag
     4441 cggtacgcgc tcgcgggcgc gaccgagccg acgatccgcg ctcacgtcga agcatgtgct
     4501 gcatgtcgtc aatgcctcga cgagatccag cgcgatgtcg tcgcgctccc gccacttcac
     4561 gtgtccgtca cgaagcgacg ccgctggtgg acgctcgcat tgcccgcctt cggcgcgctc
     4621 gcagcggccg cgatcctctt gctcgtgctg cgcccgcgcg agccgaagca ggcggacaac
     4681 atcgtccgca tcaagggtgt cggcgaagtg atcgtcgatg tcgtgcgcga gcgcggcggc
     4741 gtcgtgcgcg aggacgttcg cagctaccaa gcgggcgatc gctggaaggt cgtcgtcacc
     4801 tgcccaccaa cccatgaagt ctcgcttgcc gtcgcggttc gcgaggacgg tgccgagcac
     4861 gcggatcgtc cgctcgcccc ggcgaagatt ttgtgcggca atcgcatcta cgtgcccggc
     4921 gcgttcgagc tcaccggcgg caagccgcat cgcgtgtgcg tgcgcatcga agaccaggcg
     4981 gacgcctgcc tgacgctcgc accggaataa cgcgcaggtc tatttcttct tgaacgcggc
     5041 gagccacgtc gacgacgacg cctgcggacc gaccgcgacg cagttgtact tgccgcaccg
     5101 ccacgcggtc gcgtgcaggt cgtcgtgctt ttcgttgtgc tgcggcgcga tgccgcgacc
     5161 cttctgaacg atgtaggaga tctcgtcgct gccgagcttg agccgcatca gcgcgccatc
     5221 gcggttgagc acttccacgc gacgcgcgcc gatcacggcg gtcacgtcgg ctggcaacgg
     5281 cacgtccttg ccgaccacac cgatcgtcca tgcccgcagc tctggcatgg tcggcgcttc
     5341 gagatcccac gcaccaggct cgcgatggcg ttcgatcgct tcggtgacca gcacgttgtg
     5401 ccgcgcgggc agcaccaggt agatcgtcac gaccagcccg atcagcaccg acagcgtgaa
     5461 cagcagcgag accgagaccc gccgatgggg cggccgcggc ggcgtgacca cctggtcatc
     5521 gccttcgatc gggcctactt cttcgggttc gacgagatgc atggcgaaaa gctcggtgac
     5581 gtaccgaagc gccgaacaaa ctcctcacgt accttggcat ggaacgcggc gaggtcgaca
     5641 gcgtcacccg gcgcgatctc caggtggcgg cacaacaggg cctcgccggc atgctgctcg
     5701 gccaggtccg tggcggcggc gcgcacgttt tgggcatact cgtaatgcac catcgcgctc
     5761 ttgcccgtcg ggtaatcgag taggcccgcg gcgatccgca cctggagcac gccctcggcg
     5821 gccggtgcat gggcagcggc gtcggcgagc gggtaccacg gacagaatct cacaggggta
     5881 aggtaccgtt gccgcgtgga gcagatcgag accaggcaac gcatcgagcg cgtcgaattc
     5941 gggcgcgatc tagcgcttgc cgaccgtctc gtgcacggcg attcgctcga ggtcgccgaa
     6001 agcctgccca ccgccacgct cgacgcgatt tacatcgatc ccccgttcgg cactggcacg
     6061 gtccgccgcg gccgcggcct gcgctacacc gatcgcagcg acgatcccga ggcgttcgtc
     6121 gcgtggctga tgccgttcct cgagcagagc aagcggacgc tcgtcgagca cggctcgttg
     6181 ttcgtccacc tcgactaccg cgcggttcac tacgtcaagg tcgccctcga ccagctattc
     6241 ggacgcgatc ggtttatcaa cgagatcgtg tggtgctacg cggtcggcgg caagagccgg
     6301 cgcgggttcg ggcgcaagca cgacacgatc ttgtggtacg cgcgcagcgc ggactgggcc
     6361 ttttatcccg atgcggtccg ggtgccccgc cgcggcggct cgcacatgcg gctcgtcgat
     6421 ggcatccagg agaagaccga tcgcaagacc ggccgcgtct ataggtatcc ggtccacgcc
     6481 gggaaggtcc cggaagactg gtggaccgac atcgagacgc tcaatcactc cgaccgcgag
     6541 cgcaccggct ggccgtcgca gaagcccgag cggctgctcg agcgcctgat ctgcgccgtc
     6601 accaagccag gcgaacgaat tgcggactgg ttctcggggt cgggcacgac ggcggcggtc
     6661 gcccagcgcc ttggaaggcg atttctcgtc gtcgatcgcg aggccgacgc ggtcgcggta
     6721 tgcgcgaatc gactcctcgg gcaagggcgt gcgctggcgt cccgcgggac accgccacca
     6781 ccgttagaaa ttcttcgcta gccgcggcgc ttgcgacagg ctaaatcccg ggcgaagatt
     6841 cagcagttag gcaagtgatg acggccgaag cagcagacga ggccacgcgc gtgacgctcg
     6901 aagcgtggct cgtcacggac gtcggagtcg ttcgtgacca caacgaagac tcggcgttca
     6961 tggaaccgtc gcagggtttc ttcattgtcg ccgatggtat gggcggtcac gctgcaggcg
     7021 aagtcgcgtc ggccatggcg gtcgagaccg tgcgcacgac gctcgaagcc gcgaagcccg
     7081 agatcgaagc gttcaagcag ggcccgaccg atgccggtcg ccgcggcctc gtgcagctgt
     7141 tgcagagcgc cgttctgcaa gctcatcaat ccgtgttcca gcgcggccag gccgagaccg
     7201 acaagcaggg catgggaacc acgctcgatg tcgtgctgat cgccggcggc gaagcattcg
     7261 tcgcccacgt tggcgacagc cgcacgtacc tcgtgcgcga aggccggtcg tcgcagatca
     7321 cgactgacca caccgtcgcc gaggtgttgg tgatcgaggg caagttgacg atcgaagagg
     7381 cgcaggtgtc gccactccgc acgatcctgg tcaacgcgat cggcgtatcg gccgacgtcg
     7441 gcgtcgagat ggcgcacgtc acgctcaaga agggcgatcg gctgctgctc tgcagcgacg
     7501 gcctgcacga ctacttcccg gtcgaggacg agatcgcgca acgcatgtcg gcggagaagc
     7561 cgggcgaagc gctcaaggag atggtcgagc tcgcgaagac gcgcggcggc cacgacaaca
     7621 tcaccggcgt cgccgtgcac gtgatcgatc tgatcgaagc tgcggtgccg aaggggctca
     7681 gcgaagactc gacccaaccg gtcgacatcc agggcaaccc gtttgcggcc gacgaaccga
     7741 cgcagaccgc cgcgaacggt ggcaacaccg gaacacctcc cgagacgccg accgaggcgc
     7801 cgcccggaac gccgaccggg gggaacttcc gccggaccga gccgatgcgc gcgatcaacg
     7861 aggtcggcac gctgccgacg ctgccgccgc cgaacaagcg caaggacgac gtcaagaagg
     7921 gcgacaagaa ggacgcggcc aagaaaaccg acgtctcgac cgatgtcgcc gcagacgacg
     7981 cgcagctcga caagaagggc aagagcgcga aggacgagac gccgaaaccc gccgacaaga
     8041 aaccggacga gacgccgaag cccgccgaca aaccggacgg cgatccgaag gcggccgacc
     8101 ccaagagcga cgccaagagc gatgagagtg gcgacgccgt gcccgtcgag gccgtgggta
     8161 cggccgatac cggccgcgtc accgcaccga agaaggactg atcaggcttc gggcttcggg
     8221 ctaccgactt cgggtcggag cggatctcgg gtcggagtgg atctcgtcgg agtcggctat
     8281 tttcccgcgg gctcgcggcg ctcgacggtc gtgtctgctt cgatctcgac gcgctcgtgc
     8341 tcgcctgcgc gggcgtcgac gacgagcacg tcgccgcgcc gcgtgcgttc gaacgcgcga
     8401 tttgcatccg accgattgcg cttgagcagc cgcagcggca cgcgtcccgc atcactacag
     8461 ccgaacactt cttgcttgcc cttctgcacg aa
//
LOCUS       DASUFX010000696        10845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1361263, whole genome shotgun sequence.
ACCESSION   DASUFX010000696 DASUFX010000000
VERSION     DASUFX010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361263"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFV99_28105"
     CDS             complement(<1..571)
                     /locus_tag="VFV99_28105"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX5063285.1"
                     /translation="MGIFSRIKNGISSKANAALDKAIDPEKELEMAILELEEGRKKAL
                     QELVTYKATAKNLDNDIEKYKTKAAEWEKRAMMAVRAGDDEAAKTALREKKNAETELA
                     KIERDKHEAASYAIQLNKSRKEFEVKLQMLRLRKGTLATQLAAARSGNGDAFGNDGEV
                     WEKFARAEERIDAEAIETEVDAAIRGEEAA"
     gene            complement(588..2012)
                     /locus_tag="VFV99_28110"
     CDS             complement(588..2012)
                     /locus_tag="VFV99_28110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5063286.1"
                     /translation="MIGTIVCVDDQAEVRRLLGDVFRAKGGNVVSFDDGEDAIAWLGS
                     NEADLVILDLDLGAGRRTGIEICKAIRARLPELPIIILTGHGTIDDAVAAVKAGAVDF
                     ITKDPYLEDKLEISVEKVERIIKHALDRKRLEQENKQLRATNERLRKAAGPRWQIVGD
                     SQAMRTVMTKIEKVAPVPRPVLVLGPRGTGKELVARAIHMLSPRASEPYITINCAAVA
                     ESLLESELFGHEEGAFTGATKQKEGKFELADGGTLFLDEIGNMSLEFQAKILRVLEYQ
                     RFERVAGSESIQVNVRVIAATNADLKQAMANGTFRADLYDRLAFEVLTLPPLAQRLED
                     VAVLATYFLMRFRAEVAGITVREISGDAFDRLAQYDYPGNVRELKNVVERAVYMAQGE
                     VLRAGDIDLALPPEAKQAPVGGTDFQDDPKLALPDRVDAFEAWLCKDALERTRYKQKE
                     AAALLGLTYDQFRQRYRKYALGKE"
     gene            complement(2009..2470)
                     /locus_tag="VFV99_28115"
     CDS             complement(2009..2470)
                     /locus_tag="VFV99_28115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063287.1"
                     /translation="MDERRARMTRVIDGSDPVAGSLVRDYLNGAPALAARLEESGIPV
                     VVFVSNDRWDDSPDYFKQMQRGLDAAIAKHGRAVYVARTTAGYLLKAARRLADRRVFP
                     PLACAHGVFVGYIAVGEGGLVFFDATGKDWGVRPPSTLFEAAISGMMAPYR"
     gene            complement(2446..4314)
                     /locus_tag="VFV99_28120"
     CDS             complement(2446..4314)
                     /locus_tag="VFV99_28120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063288.1"
                     /translation="MSPLLELGDRYWALGLPAAAKSALARALATSDDVTPALRLTQIA
                     LAQGDAKSARTYATEAAKREPGPATKILLARAQLVGGEIAAARMSLSTALDSPKLKPW
                     DRARAHLELSRAADSQGDAQGAAAQAGAAFEAALTAAQKEVAELGLVEEIAAAVVAHD
                     RGADAMTSLDTVRGRPGYQLCLALVLAARQGAGDVNVTDTMVDDALAAAERELAEAGH
                     DDSALKVRRLERRARKVANIERAELIAEIESVAHTSAGAELAALDRARLHFLRASLCE
                     DDPQTRDRAEEAYRKGLTFQPGHTAAACRLALLVLDRGDQAGALAEIERALRIDASHG
                     MAWRNAARMLDAQSPSLGVVVGRLLDAANPGAGSAAAGVAPRLVTATAEVARHDVLAG
                     VYAHGHRVKNLLGIIGARTRSARKLTTEGDLHDRLRDLEKDVTALYEEWAQYLRSMQA
                     PTPTVELVPLPPLLHEVVIAAQARTQQVSIALEHTAALPDVRGDRMLLREALLNVVSN
                     AAEACATSRGEVAVRVRAIAGPAASAAPILEIVVADSGPGIPRAHLGRLFVPGFTTKE
                     TGSGVGLAIAERVVSAHHGRITVDSEEGKGTTITITLPTDKAGLAVLPRWTSAERA"
     gene            complement(4583..5281)
                     /locus_tag="VFV99_28125"
     CDS             complement(4583..5281)
                     /locus_tag="VFV99_28125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063289.1"
                     /translation="MSDVPSATVVQDGKLGGDVPKRDGGGGKPREPYKRKLSNYLLDK
                     RLQLRYILLVTVLSGVISGSLGYLIYQQRHAASESIERDLAALTQDDKSLKDFQDQVA
                     AGMAAEDRALIYKMVGVGVGLVIILSFYLVLMTHKVAGPLYKISMYFDRMKEGRLGNV
                     TALRRGDMLQDFFGNFREMHEAVRTRLQSDVITMESAAVALRAKATADGRQGLVDALD
                     ALDKHITARKKQLL"
     gene            complement(5274..6473)
                     /locus_tag="VFV99_28130"
     CDS             complement(5274..6473)
                     /locus_tag="VFV99_28130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063290.1"
                     /translation="MAGAKPQYKRSWKNLLLNKRYQLRFTLFMVGVSAVLMTGLGVWV
                     MKEANEATTVAMARVRGEACPKIPEVIEVPGTTDEPAVPMNIEDAPPAAPEAPAEPPT
                     DEKAAPVKDEKAAPAKDDKARDKAPAADEAAEDKPAEPKEAKPELSKVDPKHVAANNG
                     QADLLAVKALWCTDAECKPETAAPLLIKVKKCDDYVKDKLANAEAVDALRKTSIPVVK
                     CEGGGEPYSVADAEPERHATVQLEETSMTITPTLPTDFADRIVAHHTCEMRQAGSINA
                     LEQGRLRILWVLIGTGLLLMLGLALYGIKMTHKVAGPLFKVSLYFAKMRDGRLDKVYN
                     LRKGDQLVDFYEHFKSAHAGVVTMEKDDLARIKATIEAADAAGLGDHASIAELRTLLA
                     RKEKSLE"
     gene            complement(6473..7348)
                     /locus_tag="VFV99_28135"
     CDS             complement(6473..7348)
                     /locus_tag="VFV99_28135"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5063291.1"
                     /translation="MLSKIASRLIRFAAPLVVIAMLFVSSVARADSVDTLIKQLSDSS
                     DKVRLAAALNIAKAGDERGILPLAKTLLNDSDKNVRGAAAVGLGKLIKSNPNTKYKSL
                     ATANLKTAAANDASDFVKTQASNALITIGSGGGSTTTQPSGGGGIYVNVGPMSSKTTS
                     SKNDKLRALMVKTAQKTLTKVASNMSQTWPSGTPSKADLAKKGVSGFYVDGTLNTLDI
                     KTSGGGATISCKVSMLLADFPDKSVFGFLNGGASVTASSSKTDQDLASEDCVTAVIEN
                     LITSKIVPTIKSKVP"
     gene            complement(7412..7936)
                     /locus_tag="VFV99_28140"
     CDS             complement(7412..7936)
                     /locus_tag="VFV99_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5063292.1"
                     /translation="MAATIRAYAGKRPELGPSVFLAETCAVIGDVVIGEESSIWYGTV
                     IRGDVMPIRIGARTSIQDNSVIHVTSEFSGTIIGNDCTVGHNAIIHACTVEDECLIGM
                     GAILLDGAVIGRGSLVGAGALVTPGTVIPPGSLVVGSPAKVKRPINDKEREQITYGAH
                     HYVELTRRYLAGEL"
     gene            complement(7952..8812)
                     /locus_tag="VFV99_28145"
     CDS             complement(7952..8812)
                     /locus_tag="VFV99_28145"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5063293.1"
                     /translation="MRWLGLFAVVGFASIANAAPSSNPAFLGISMEDHGVGCQVKGVT
                     AGSAAQDAGLREWDLVLAIDGVRTPNCTSLRTQIIANTPGHIVKLDVRRGTEKIALQA
                     PLSTRAEVLHRRLVGHEMESTNVVDADDSKHSYDLADSRGKVTVIGWFMLERCAGCSA
                     VFDRIADGLTSRLKGSETAPFVLAVSPQPNLSNTVAQTTQAKLTALPPVRKSFGFTTA
                     VPLAIASEETFTDLAIDDPERIHFMVVDCRGVVRFVAPIAPGSDDIDAAIDEVLAAAE
                     QAEHSRTTRR"
     gene            8923..9339
                     /locus_tag="VFV99_28150"
     CDS             8923..9339
                     /locus_tag="VFV99_28150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5063294.1"
                     /translation="MKRRLPDLAHVLDDVRPMSSVPVKAIEKRIHTRFDKAFLVVIGS
                     ELYGDSFAIARNISAGGLLVEMSYAPPLGTIVTVHFQYMREGDVLDEIVARAEVKHHH
                     YLNFTGGNDAASARAIGLRFIEFLDPGDRVEAERIH"
     gene            9402..>10845
                     /locus_tag="VFV99_28155"
     CDS             9402..>10845
                     /locus_tag="VFV99_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063295.1"
                     /translation="MRAALLLAMIAATSVAIAAPKAKKPATAKPGSGSGSAVDATKPE
                     PPPPPPPKPVDPKVQAIVDRVAKGDKKAIDELNAIAVTNYEDLANFLGRPHTATIEER
                     RAVLAGINAQVPDEKGHFVAPQRKSAKEEQADDNLDWLAELQKVDPATPGLGEVLADD
                     ASIRALAASKEIRAGYILFDAAFGAETMIYRDEVGRYLRKMHPYSIPALFRESARRDY
                     DRKRYSNYQLERLDRQEPGKALAAAAGDENLRIAILDVWGQTMYREAVHAVWTQVNAD
                     SLRTRTKARETWKQYTEGKAPPPAPKKKIQLAGGKTTKEEKALYLTSRQLADNELRKY
                     SNELLGTDYPIVEPVMDGSDEKPGYRPPKEVKIKLSEVTQQLWDYYDKQRAEVDAKQW
                     AEADAKSKAGDLATATMLLDRLLAANPDHTNKAQMANVYFTYAKELEKKAKWGEASAA
                     YSKAHGVAPQGPNAVDALAAYHYTLGKALEA"
BASE COUNT         1734 a         3681 c         3478 g         1952 t
ORIGIN      
        1 ccgccgcctc ctcgccgcgt atcgccgcgt caacctcggt ctcgatcgcc tcggcgtcga
       61 tccgctcctc ggcgcgcgcg aacttctccc acacctcgcc gtcgttgccg aacgcgtcgc
      121 cgttgccgga ccgcgccgcc gcgagctggg tcgcgagcgt gcccttgcgg agccgcagca
      181 tctggagctt gacctcgaac tccttgcggc tcttgttgag ctggatcgcg tagctcgcgg
      241 cctcgtgctt gtcgcgctcg atctttgcga gctcggtctc ggcgttcttc ttctcgcgca
      301 gcgcggtctt cgcagcctcg tcgtcgcccg cgcgcaccgc catcatcgcg cgcttctccc
      361 actcggccgc cttcgtcttg tacttctcga tgtcgttgtc gaggttcttc gcagtcgcct
      421 tgtacgtgac gagctcctgc agcgccttct tgcgaccctc ttcgagctcc aggatcgcca
      481 tctcgagctc tttctccgga tcaattgcct tgtcgagcgc ggcgttcgcc ttgctcgaga
      541 tgccgttctt gatccgcgaa aagatgccca tggccggagt ttagcgccta ttccttgccg
      601 agcgcgtatt tgcggtagcg ctgacggaac tgatcgtacg tgaggcccaa gagcgcagcg
      661 gcctccttct gcttgtagcg cgtacgttcg agtgcgtcct tgcacagcca cgcctcgaac
      721 gcgtcgacgc gatcgggaag cgccagcttc ggatcgtcct ggaagtccgt gccgccgacc
      781 ggtgcttgct tcgcctcggg cggtaacgcg aggtcgatgt ccccggccct gagcacctcg
      841 ccttgcgcca tgtacaccgc gcgctcgacg acgttcttca gctcgcgcac gttgccggga
      901 tagtcgtact gcgcgagccg atcgaacgca tcgccgctga tctcgcggac cgtgatgccg
      961 gcgacctcgg cgcgaaagcg catcaggaag tatgtcgcga gcacggcgac gtcctccaag
     1021 cgctgcgcga gcggcggcag ggtgagcact tcgaacgcca gccggtcgta tagatcggca
     1081 cggaaggtgc cgttggccat cgcttgcttg aggtcggcgt tcgtcgctgc gatcacgcgc
     1141 acgttgacct ggatcgactc gctgcccgcg acgcgctcga accgctggta ctcgagcacg
     1201 cgcaggatct tggcctggaa ctcgagcgac atgttcccga tctcgtcgag gaatagcgtg
     1261 ccgccgtcgg cgagctcgaa cttgccttcc ttctgcttcg tcgcgccggt gaacgcaccc
     1321 tcttcgtgac cgaacagctc gctctcgagg aggctctcgg cgaccgcggc gcagttgatc
     1381 gtgatgtacg gctcgctcgc gcgcggtgac agcatgtgga tcgcgcgcgc gaccagctcc
     1441 ttgccggtgc cgcgcggtcc gagcacgagc acgggacgcg gcacgggcgc gaccttctcg
     1501 atcttcgtca tcaccgtgcg catcgcctgc gagtcgccga cgatctgcca gcgcggcccg
     1561 gccgctttgc gcagccgctc gttggtcgcg cgcagctgct tgttctcttg ctcgagtcgc
     1621 ttgcggtcga gcgcgtgctt gatgatgcgc tcgactttct cgacggagat ttcgagcttg
     1681 tcctcgaggt acggatcctt ggtgataaag tcgacggcgc ccgccttcac cgccgcgacc
     1741 gcgtcgtcga tcgtgccgtg accggtgagg atgatgatcg gtagctccgg caaccgcgcg
     1801 cggatcgcct tgcagatttc gatgccagtg cggcggccag cgccgagatc gagatcgagg
     1861 atgacgaggt cggcctcgtt cgagcccagc cacgcaatcg cgtcctcgcc atcatcgaag
     1921 ctgacgacgt tgccgccttt cgcgcgaaac acgtcgccga gcagccggcg cacttcggcc
     1981 tggtcatcga cgcaaacgat cgtcccgatc atcgatacgg cgccatcatc ccgctgatcg
     2041 cggcctcgaa caacgtcgac ggcggccgaa caccccagtc cttacccgtg gcgtcgaaga
     2101 acacgagacc tccctcgccg accgcgatgt agccgacgaa tacgccgtgc gcacacgcga
     2161 gcggcggaaa cacgcggcga tccgccagcc ggcgtgccgc tttgagcaag tagcccgcgg
     2221 tcgtgcgcgc gacgtagacc gcacgcccgt gcttcgcgat cgccgcatcg agaccgcgct
     2281 gcatctgctt gaagtagtcc ggcgagtcgt cccaccgatc attcgatacg aacaccacga
     2341 ccgggatgcc ggactcctcg agccgcgccg cgagcgccgg tgctccattg aggtagtcgc
     2401 ggacgaggct gccggcgacg gggtcgctgc cgtcgatcac ccgcgtcatg cgcgctcggc
     2461 gctcgtccat cgcggcagca cggcgagccc tgccttgtcc gtcggcaacg tgatcgtgat
     2521 ggtcgtgccc ttaccttctt ccgagtcgac ggtgatccgg ccgtggtgcg ccgacacgac
     2581 gcgctcggca atcgcgagcc cgacgccgga gccggtttcc ttcgtcgtga agcccggcac
     2641 gaacaagcgg ccgaggtgtg cgcgcggaat gcccggaccg ctatccgcga cgacgatctc
     2701 gaggatcggc gctgcactcg cggccggccc cgcgatcgcg cgcacgcgaa ccgcgacctc
     2761 gccccgcgac gtcgcgcagg cttccgcggc attcgacacc acgttgagca gcgcctcgcg
     2821 cagcagcatg cgatcgccgc gcacgtccgg cagtgccgcc gtgtgctcga gcgcgatcga
     2881 cacttgctgg gtacgcgcct gcgctgcgat cacgacttcg tggagtagcg gcggcagtgg
     2941 cacgagctcg acggttggcg tcggtgcctg catcgagcgc aggtactgag cccattcctc
     3001 gtaaagtgcg gtgacgtcct tttcgagatc gcgcagccga tcgtggaggt cgccctcggt
     3061 cgttagcttg cgtgcgctgc gggtgcgcgc accgatgatg ccgagcaagt tcttcacccg
     3121 gtgaccgtgc gcgtagacgc cggcgagcac gtcgtgacgc gcgacctccg ccgtcgcggt
     3181 gacgagccgc ggcgcaacac cggcagccgc cgagcccgcg cccggattcg ccgcgtcgag
     3241 cagcctgccg acgacgacac cgaggctcgg cgattgcgcg tcgagcatgc gcgcggcgtt
     3301 gcgccacgcc atgccgtgac tggcgtcgat gcgcagcgcg cgttcgatct cggcgagcgc
     3361 gccggcctga tcgccgcgat cgagcacgag cagcgcgagc cgacaggcag ccgccgtgtg
     3421 gcccggctgg aacgtgagcc ctttgcgata cgcctcctcg gcgcgatcgc gcgtctgggg
     3481 atcgtcttcg cacaagctcg cgcgcaggaa gtggagccgc gcgcgatcga gagctgctag
     3541 ctcggcaccc gcgctcgtgt gcgcgacgct ttcgatctcg gcgatcagct cggcgcgctc
     3601 gatgttcgcg accttgcgcg cgcgccgctc gagccggcgc accttgagcg ccgagtcatc
     3661 gtggccggcc tccgcgagct cgcgctcggc agctgcgagt gcatcgtcga ccatcgtgtc
     3721 ggtgacgttg acatcgcccg ctccctgccg cgccgcgagc acgagcgcga gacacagctg
     3781 atagccaggc cgaccgcgca cggtatcgag gctcgtcatc gcgtcggcgc cgcgatcgtg
     3841 tgcgaccaca gccgcggcga tttcctccac gaggccgagc tccgcgacct ctttttgcgc
     3901 ggcggtcaac gccgcttcga acgcggctcc cgcctgcgcg gccgcgccct gcgcgtcgcc
     3961 ttgcgaatcc gcggcccgcg atagctcgag gtgcgcgcga gctcgatccc atggtttcag
     4021 cttcggcgag tcgagcgcgg tcgacaggct catgcgcgcc gctgcgatct cgccgccgac
     4081 gagctgtgcc cgcgcgagca ggatcttcgt cgctgggccg ggctcacgct tcgcggcctc
     4141 cgtcgcgtac gtgcgcgcgc tcttcgcgtc gccctgcgcc agcgcgatct gggtcagccg
     4201 cagcgcgggc gtgacgtcat cactcgtcgc gagcgcacgc gcaagcgcac tcttcgccgc
     4261 ggctggcagt ccgagcgccc agtatcggtc gccgagctcg agcagtgggc tcacgcagcg
     4321 atggtatccc atccctcgta ggcgtaagcg taagcgcaag cgatcagggt gcgtcgcctg
     4381 cggcgcgccg agctacagcg tcgccacagc agcatgctcc gatcgggaga gccgtgcgga
     4441 tcgaacatcg ccggtcatcg caacgcgagc agaacatgga tccaccgcac gcgtcgcagc
     4501 tcctgagacg ctcgctgcca gtcgccttcg gaatgacgca gaacggggcg gttgccgctt
     4561 acgcttacgc cgacttacgt aattatagca actgcttctt gcgtgcggtg atgtgcttgt
     4621 cgagcgcgtc gagcgcatcg acaagacctt gtcggccgtc ggccgtcgcc ttcgcgcgca
     4681 gcgcgactgc agcactctcc atcgtgatca cgtcggactg caggcgtgtt cgaaccgctt
     4741 cgtgcatttc tctgaagttg ccgaagaagt cttgcagcat gtcgccgcgt cgtaaggccg
     4801 tcacgttgcc gagccggccc tccttcatcc gatcgaagta catcgagatc ttgtagagag
     4861 ggcccgccac cttgtgcgtc atcagcacga ggtagaacga caagatgatc acgaggccga
     4921 cgccgacgcc gaccatcttg tagatcagcg cgcggtcctc ggcggccatg cccgcagcga
     4981 cctgatcctg gaagtccttg aggctcttgt cgtcctgcgt gagcgccgcg aggtcgcgct
     5041 cgatcgactc cgacgccgcg tgccgctgtt gatagatcag gtagccgagc gagccgctga
     5101 tcacgcccga caggacagtg acgagcagga tgtagcggag ctggagccgc ttatcgagca
     5161 ggtagttcga cagcttgcgc ttgtacggct cgcgcggctt gccaccgccg ccatcgcgct
     5221 tcggcacgtc tccgccgagc ttgccgtcct ggaccaccgt ggcggagggt acgtcactca
     5281 agggacttct ccttgcgcgc cagcagcgtg cgcagctccg cgatcgacgc gtgatcgccg
     5341 agaccggccg cgtcagccgc ttcgatggtg gccttgatgc gcgcgaggtc gtctttctcc
     5401 atcgtcacga cgcccgcgtg cgcgctcttg aagtgttcgt agaagtcgac gagctggtcg
     5461 cctttgcgca ggttgtagac cttgtcgagg cggccatcgc gcatcttcgc gaagtagagg
     5521 ctgaccttga acagcgggcc ggccaccttg tgcgtcatct tgatgccgta gagcgcgaga
     5581 ccgagcatga gcaagaggcc ggtgccgatc agcacccaga ggatgcgcag ccggccctgc
     5641 tcgagcgcgt tgatcgaccc cgcctgccgc atctcgcacg tgtggtgcgc gacgatgcga
     5701 tcggcgaagt cggtcggcag cgtcggcgtg atcgtcatcg acgtctcttc gagctgcacc
     5761 gtcgcgtgac gctccggctc ggcgtcggcc acgctgtacg gctcgccacc gccctcgcac
     5821 ttgacgaccg ggatcgacgt cttgcgcaac gcatcgacgg cttcggcgtt cgcgagcttg
     5881 tctttgacgt agtcgtcgca cttcttgacc ttgatcaaga gcggcgctgc cgtctcgggc
     5941 ttgcactcgg cgtcggtgca ccacagcgcc ttgaccgcga gcagatccgc ctgcccgttg
     6001 ttcgcggcga cgtgcttcgg atcgaccttc gacagctcgg gcttcgcttc tttcggctcg
     6061 gccggtttgt cctcggcggc ttcgtcggct gcaggcgctt tgtccctcgc cttgtcgtcc
     6121 ttcgcgggcg cagccttctc gtcctttaca ggcgcggcct tctcgtccgt cggcggctcg
     6181 gccggcgctt cgggcgcagc gggcggcgcg tcttcgatgt tcatcggcac cgccggctcg
     6241 tcggtcgtgc ccggtacctc gatcacttca gggatcttgg ggcacgcttc gccgcgcacg
     6301 cgggccatcg cgaccgtcgt cgcttcgttg gcctctttca tcacccagac gccgaggccc
     6361 gtcatcagca cggccgacac gccgaccatg aacagcgtaa agcgcagctg atacctcttg
     6421 ttgagcaaga ggttcttcca gctgcgcttg tactgtggtt tggcgccggc catcacggaa
     6481 ccttggattt gatcgtcggc acgatcttag acgtgatcaa gttctcgatc acggcggtga
     6541 cacaatcctc gcttgcgagg tcttgatcgg ttttcgacga gctcgctgta acagacgcac
     6601 cgccgttgag gaagccgaac acgctcttgt ccggaaagtc cgcgagcaac atgctgactt
     6661 tacacgagat ggtcgcgccg ccaccggagg ttttgatgtc gagagtgtta agcgtgccgt
     6721 cgacgtagaa gccggagacc cccttcttcg cgaggtcggc cttgctgggg gtcccgctgg
     6781 gccacgtctg gctcatgttg ctcgccacct tcgtcagcgt cttttgcgcc gtcttgacca
     6841 tcaacgcccg cagcttgtcg ttcttactgc tggtcgtctt gctcgacatc gggccgacgt
     6901 tcacgtagat gccgccgccg ccgctcggct gcgtggtcgt gctcccgccg ccgctgccga
     6961 tggtgatcag cgcgttgctc gcctgggtct tcacgaagtc gctcgcgtcg ttcgcggccg
     7021 ccgtcttgag gttcgcggtc gcgaggctct tgtacttggt gttcgggttc gacttgatga
     7081 gcttgccgag accgaccgcc gcggcgccgc ggacgttctt gtcgctgtcg ttgagcagcg
     7141 tcttcgcgag cggcaggatg ccgcgctcgt ctcccgcctt cgcgatgttc agtgcagccg
     7201 cgaggcgaac cttgtccgac gagtccgaca gctgcttgat cagggtgtcg accgagtcag
     7261 cgcgcgcgac actcgagacg aacagcatcg cgatcaccac gagcggtgcc gcgaatcgga
     7321 tcaaacgaga ggcgatcttc gagagcatta cgtcttcgtg ttcccctgag acgtacgacc
     7381 agcccgctac ttcaatccag agtaacgact tttacagctc gcctgcaagg tagcgacgcg
     7441 tcagctctac atagtggtgg gcgccgtacg ttatttgctc ccgttccttg tcgttgatcg
     7501 gccgcttcac cttcgcgggt gagccgacta cgagactgcc gggtgggata actgttcccg
     7561 gtgtgaccag cgcgcctgca ccgacgaggc tgccgcgacc gatcaccgca ccgtcgagca
     7621 agatcgctcc catcccgatc aggcattcgt cttcgaccgt gcacgcgtga atgatcgcgt
     7681 tgtggccgac ggtgcaatcg ttgccgatga tcgtgccgga gaactcgctc gtcacgtgaa
     7741 tgacgctgtt gtcctggatc gacgtgcgtg cgccgatccg gatcggcatc acatcgccac
     7801 gaatgacggt gccgtaccag atcgacgatt cctccccgat cacgacgtcg ccgatcaccg
     7861 cgcaggtttc agcgagaaat acgctagggc cgagctcggg gcgtttgcct gcgtatgcgc
     7921 gaatcgtggc ggccatgccg cggacgataa cctaacgacg cgtcgtgcga gagtgctcgg
     7981 cctgctcggc cgccgcgaga acctcgtcga tggcggcatc gatgtcatcc gagcctggcg
     8041 cgatcggcgc gacgaagcgc acgacaccgc ggcagtcgac gaccatgaaa tggatgcgct
     8101 cgggatcgtc gatcgcgaga tcggtgaacg tctcctcgct ggcgatggcg agcggcaccg
     8161 ccgtcgtgaa cccgaaggat ttgcgcacgg gcggcagtgc ggtgagcttg gcttgcgtcg
     8221 tctgcgcgac ggtgttcgag aggtttggct gcggcgacac cgcgagcacg aacggtgcgg
     8281 tctcgctgcc cttgaggcgg ctcgtcaggc cgtcggcgat gcgatcgaac acggcgctgc
     8341 aacctgcaca gcgctcgagc atgaaccagc cgatcacggt gaccttgccg cgcgagtcgg
     8401 cgaggtcgta gctgtgcttg ctgtcgtcgg cgtcgacgac attggtcgat tccatctcgt
     8461 gaccgacgag ccggcgatgc agcacttcgg cgcgcgtcga cagcggcgcc tgcagggcga
     8521 tcttctcggt gccgcgtcgg acatcgagct tcacgatgtg accgggcgtg ttcgcgatga
     8581 tctgcgtgcg cagcgacgtg cagttcggcg tgcgcacgcc gtcgatcgcg agcaccagat
     8641 cccactcgcg gagcccggca tcctgggcgg cgcttccggc ggtcacgccc ttcacctgac
     8701 agccgacccc gtgatcctcc atgctgatgc cgaggaacgc ggggttactc gacggtgccg
     8761 cgttcgcgat ggacgcgaat ccgacgacgg cgaagagccc gagccagcgc acgtgtggag
     8821 cctgcctgaa cgacgtacga agggccagag gtttcgtgtg ttcccatgta ggacaaggtg
     8881 agatttttga ctccatgacc ggggcgctcg atcattattt cggtgaagcg ccgcctgccc
     8941 gatctcgccc atgtcctcga cgacgttcgt ccgatgtcct cggtgccggt caaggccatc
     9001 gagaagcgca tccatacccg cttcgataag gcgttcctcg tcgtgatcgg cagcgagctg
     9061 tacggcgact cgttcgcgat cgcgcgcaac atctccgcgg gcgggttgct cgtcgagatg
     9121 agctacgcgc cgccgctcgg caccatcgtc acggtgcact ttcaatacat gcgcgaaggc
     9181 gacgtgctcg acgaaatcgt cgcacgcgcc gaggtcaagc atcaccacta cctcaacttc
     9241 acaggcggaa atgacgccgc gagcgcgcgc gccatcggcc tgcgtttcat cgaatttctg
     9301 gatccgggag atcgcgtcga ggccgagcga attcattagt cagcgtacgc gttgtctccg
     9361 ctaacttgcg aagtcggcat cgaacggcta agtccaggac tgtgcgagcc gcgcttctcc
     9421 tcgcgatgat cgcggcaacg tctgtcgcga tcgccgcccc caaggcaaag aagcccgcga
     9481 ccgcgaaacc gggcagcggc agcggcagcg ctgttgacgc cacgaagccc gagccacctc
     9541 ctccgccgcc gcccaagccg gtcgatccga aggtgcaggc gatcgtcgat cgcgtcgcca
     9601 agggcgacaa gaaggcgatc gacgagctga acgcgatcgc cgtaacaaat tacgaggacc
     9661 tcgcaaactt cctcggccgg ccacacacgg cgaccatcga agagcgccgc gcagtgctcg
     9721 cgggaatcaa cgcgcaggtg cccgacgaga aggggcactt cgtcgcgccg cagcgcaaga
     9781 gcgccaaaga agaacaagcc gacgacaacc tcgattggct tgccgagctg cagaaagtcg
     9841 atccggccac acccggcctc ggcgaagtgc tcgccgatga cgcctcgatc cgcgcgctcg
     9901 ccgcgtcgaa ggagatccgc gccggctaca tcctgttcga cgccgcgttc ggcgccgaga
     9961 cgatgatcta tcgcgacgag gtcggccgct acctgcgcaa gatgcacccc tactcgattc
    10021 cggcgctgtt tcgtgaatcg gcgcgccgcg actacgaccg caagcgctac tcgaactacc
    10081 agctcgagcg cctcgatcgg caggagccgg gcaaggccct tgcggcggcc gccggcgacg
    10141 agaacctgcg gatcgcgatc ctcgacgtct ggggccagac catgtatcgc gaggccgtcc
    10201 acgcggtgtg gacgcaggtg aacgcagact cgctgcgcac gcgcacgaaa gcgcgcgaga
    10261 cctggaagca gtacaccgaa ggcaaggcac cgccgcccgc gccgaagaag aagatccagc
    10321 tcgccggcgg caagacgacg aaagaagaga aggcgctgta cctgacgtcg cgccagctcg
    10381 ccgataacga gctgcgcaag tactccaacg agctgctcgg caccgactat ccgatcgtcg
    10441 agccggtgat ggacggcagc gacgagaaac ccggctaccg accgccgaaa gaagtgaaga
    10501 tcaagctctc cgaggtcacg cagcagctct gggactacta cgacaagcag cgcgccgagg
    10561 tcgacgcgaa gcaatgggcc gaggccgacg cgaagtcgaa ggccggcgac ctcgcgaccg
    10621 cgacgatgct gctcgaccgc ctgctcgccg ccaatcccga tcacacgaac aaggcgcaga
    10681 tggcgaacgt gtacttcacg tacgccaagg agctcgagaa gaaggcgaag tggggcgaag
    10741 cgtccgcggc ctactcgaag gcgcacggcg tcgcgccgca gggaccgaac gcggtcgacg
    10801 cactcgccgc atatcactac acgctcggca aggcgctcga agcac
//
LOCUS       DASUFX010000697         6015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_805665,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000697 DASUFX010000000
VERSION     DASUFX010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805665"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFV99_28160"
     CDS             <1..963
                     /locus_tag="VFV99_28160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063296.1"
                     /translation="RGKQRVVLVPEGVTQLKLAGRKVGAKELAPLEAKTITWSTVEPH
                     GFRETKAQNGATSDFYSNVSTDAKTFGQWLGYDHIDYFEHVFAEHGDPIITAEITPSE
                     AGAVQVPKLGTLRFKVDVELADGSHVASPGAEATDTFGILANVHRVSVRASDDFLGWL
                     GAYLLVPEVFGSAGPGNNHQTERFVGADCADVMVGAMRRMGKKLAYTNVAGLPAYTTT
                     VGAATELDDHGMPATPIDGIKPGDLIRIDYGGVLRHHTPRDWDHVAALWEDRSDPKGP
                     LKGKADGKLDGFDLVIHVGHPRLKIEPLSEQAPATIDVLRWRAR"
     gene            988..1917
                     /locus_tag="VFV99_28165"
     CDS             988..1917
                     /locus_tag="VFV99_28165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063297.1"
                     /translation="MDASRASLPDVIAHLRAAAGPTVEVDVTDPDLGRGTYAGESVAH
                     AGVVYVHRPFRVWVDLAERLGFRLLTPRPLAPPLVRLVFERLDRAARLRPDTGDATEK
                     YGANSEFARISKLEDPGFVIDMADALARVAPDPNARVLDLGVNTGDELALLMTLSPAL
                     RTATLVGIDHSASAIARARERFAGAPNVQLHEADLSTLGALDLGRFDLVLSIGTLQSP
                     GIDDREVVRQIVQNHLAPTGAVIIGVPNCRYLDGEVEYGTRMKNFTQPELGLLVKDVA
                     FYRKYLQQHHRQVYVTGKHYILVTGVADQDANT"
     gene            complement(1899..2438)
                     /locus_tag="VFV99_28170"
     CDS             complement(1899..2438)
                     /locus_tag="VFV99_28170"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5063298.1"
                     /translation="MSCATPLTDEQLVAYWADDLDEAALAQVEEHLFACDECLANATH
                     VQQVAQAFRGLPPVISHAELAALRARGLTIVDNDFIAERRTPVTFERHVDFMIHHLTG
                     LDLTAAERVEVIVRDESGKVMFEEPIAPFDPQSGEILIACQRHFAAFPPDVVFDVRVH
                     RAAAPPALSTFCIPHVFAS"
     gene            complement(2435..2947)
                     /locus_tag="VFV99_28175"
     CDS             complement(2435..2947)
                     /locus_tag="VFV99_28175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5063299.1"
                     /translation="MRRVAAAGRDHAAEAELCRRFAPRVRLYGLKHLRDEDRARELVQ
                     VVLVAMIEAMRAGRVEDPERFDRFVLGMARNLASRARHAETRAVPTDVAELDVESVLP
                     RHDELDIEALLHCLQRLETRARTVLHLSFYREKSADEIAAVLETSVGNVRVVRHRAVA
                     QLRDCLGAAS"
     gene            complement(3009..3746)
                     /locus_tag="VFV99_28180"
     CDS             complement(3009..3746)
                     /locus_tag="VFV99_28180"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063300.1"
                     /translation="MTVTNSQSGTNVHEIAPSIYRISTPVPPNPGLPGGFTFNQFLIV
                     DDAPLLFHTGLRKMFPLVRQAVEHVLGDVAKLRYVGFSHFESDECGSLNDWLAAAPQS
                     QAVCSMIGAMVSVEDYAIRAPRPLADGEELSLGTRKVRWLDAPNLPHNWECGYMFEST
                     SRTLLCGDLFTHAGADVAPITEQEVLGPSEAMRAAMGGVAIEANTRAILEKLARTEPT
                     TLALMHGSSYRGNGAALLKGLADALGV"
     gene            complement(3805..5835)
                     /locus_tag="VFV99_28185"
     CDS             complement(3805..5835)
                     /locus_tag="VFV99_28185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5063301.1"
                     /translation="MLRVVLAAAVLMLTLGRASAQSSLAALPKDPEAEKQFRADVAAV
                     SAEAAAAYDEGNAARDADRVDDALAAYRRAAELASNVDHPHRRVCGVLFAKAQLDDAV
                     VECEKALSLVPSSPYDKGALAKALMRRNRAEDVGRYTDLAREAAEAKPNDVTLNIIWC
                     IAGLQSKNDVGFARCSKHLLAIDPNGVQANFFAVGGALAERDFDEAHGYLEKAKQAGL
                     DQYTYDGMNKQIEEAEQKQQEPIIDVDKYLEPGLLTFCLWGLVMLVLFGVGNALSRAT
                     LRAVDRNDSLDATGTPRERRLHKIYKAVLGLAGLYFYLSLPLMIVAVIAAGFFALYVF
                     EEIGAIPVYVIVLVVVMIFGTIIAVVRSLFLRREPDPRSERIVLTAYPKLHAMLDEVA
                     AAIGTRPVDVVHLVPGTDIAVMERTGLLRTILRRPSERRLILGVAQFDSMTQLQLRAV
                     LAHEYGHFRNEDTAGGGFAIAVRRSLFSVVERMAKSGANTIYNPVWWFLRGFWRMYLG
                     ISQGASRLQEVLADRWSIRAYGNAAFVAGYRHVVARTVEFDGDLGAKVNEVLEKDWSL
                     VNLYDWDPEAKTSPDQLAIAVDKAMNREPSPYDSHPSPRQRIDWASRHDVPREPQADD
                     AAPVWDLFPDPEKIERMMTTIVRENVQRVSGRIISDKDDAEEPEAESADSAS"
BASE COUNT         1045 a         2011 c         1954 g         1005 t
ORIGIN      
        1 cgcggcaagc agcgcgtggt cctcgtaccc gaaggagtca cgcagctgaa gctcgccggt
       61 cgcaaggtcg gcgcaaagga gctcgcgccg ctcgaggcca agacgatcac gtggtcgacg
      121 gtcgagccgc acggcttccg cgaaaccaaa gcgcagaacg gtgcgacgag cgacttctac
      181 agcaacgtct cgaccgacgc gaagacgttc ggccagtggc tcggctacga ccacatcgat
      241 tacttcgagc acgtgttcgc cgagcacggc gatccgatca tcaccgccga aatcacacca
      301 agcgaagccg gggcggtgca ggtgcccaag ctcggcacgc tgcgattcaa ggtcgacgtc
      361 gagctcgcgg atggctcgca cgtcgcgagt cccggcgccg aagcgaccga tacgttcggc
      421 atcctggcga acgtgcatcg cgtgtcggtg cgcgcgagcg acgactttct cggctggctc
      481 ggcgcctatc tcctcgtgcc cgaggtattc ggctcggcgg ggcctggcaa caatcaccag
      541 accgagcgct tcgtcggggc cgactgtgca gacgtgatgg tcggcgcgat gcggcgcatg
      601 ggaaagaagc tcgcgtatac gaacgtcgcc gggctacccg cgtacacgac gaccgtaggc
      661 gccgcgacgg agctcgacga tcacgggatg ccggcgacgc cgatcgatgg catcaaaccg
      721 ggtgacctca tccgcatcga ctacggcggc gtgctgcggc atcacacgcc acgcgattgg
      781 gatcacgtcg cagcgctgtg ggaagaccgc agcgatccga agggtccgct caaaggcaaa
      841 gccgacggca agctcgacgg cttcgatctc gtgatccacg tcggtcatcc gcgcctgaag
      901 atcgagccgc tctcggagca agcgccagcg acgatcgacg tgctgcgctg gcgagcgcgc
      961 tagccgtatt catggcaccg tccgtgcgtg gacgcttcgc gcgcatcgct gcccgacgtg
     1021 atcgcgcatc tgcgcgcggc ggctgggcct accgtcgaag tcgatgtcac cgatccagac
     1081 ctcggtcgcg gcacgtacgc cggcgagtcg gtcgcgcatg cgggtgtcgt ttacgtccat
     1141 cgaccgttcc gcgtctgggt cgacctcgcc gagcgtctcg gcttccgcct gctcaccccg
     1201 cgcccgctcg ctccgccgct cgttcgtctc gtgttcgagc gtctcgatcg tgctgctcgc
     1261 ctccgccctg acactggcga cgccaccgag aagtacggcg cgaactcgga gttcgcgcgg
     1321 atctcgaagc tcgaggatcc agggttcgtg atcgacatgg ccgacgcgtt ggcgcgcgtc
     1381 gcgccagatc ccaacgcacg cgtgctagac cttggcgtca acaccggcga cgagctcgcg
     1441 ctgttgatga cgctatcgcc cgcgctgcga acagcgacgc tggtcggcat cgatcacagc
     1501 gcctcggcga tcgcgcgagc gcgtgaacgt ttcgccggtg cgccgaacgt gcagcttcac
     1561 gaggctgacc tctcgacgct cggcgcactc gacctgggtc gcttcgatct cgtgctgtcg
     1621 atcggcaccc tgcagagccc cggcatcgac gaccgcgagg tggttcgcca gatcgtgcag
     1681 aaccatctcg ctcccaccgg tgcggtgatc atcggcgttc cgaactgccg ctacctcgac
     1741 ggcgaggtcg agtacggcac gcgcatgaag aacttcacgc aacccgagct cggcctactc
     1801 gtcaaggacg tcgcgttcta tcggaagtat ctccagcagc atcaccgcca ggtctacgtc
     1861 accggcaagc actacatcct cgtcaccggc gttgcggatc aggacgcgaa cacgtgagga
     1921 atgcagaagg tcgagagcgc gggcggcgct gcagcgcgat ggacgcgcac gtcgaacacg
     1981 acatcgggcg ggaacgcggc gaagtgacgc tggcacgcga tcagaatctc gccgctctgc
     2041 ggatcgaacg gcgcgatcgg ctcttcgaac atcaccttgc cgctttcatc gcggacgatc
     2101 acctcgacgc gctcggcggc cgtcaagtcg aggccggtga ggtgatggat catgaagtcg
     2161 acgtggcgct cgaacgtgac cggcgtgcgg cgctcggcga tgaagtcgtt gtcgacgatc
     2221 gtcaggccgc gtgcgcgcaa cgcagcaagc tccgcgtgcg agatcaccgg tggcaggcca
     2281 cggaacgcct gcgcgacctg ttgaacgtgc gtggcattcg cgaggcactc gtcgcacgca
     2341 aacaggtgtt cttcgacctg cgcgagcgcg gcctcgtcga gatcgtcggc ccagtaggcg
     2401 acgagctgct cgtcagtcag cggcgtcgcg caactcatga cgccgctccg aggcaatcgc
     2461 gcagctgcgc gaccgcgcga tgacgaacaa cgcgcacgtt gccgacgctc gtttcgagga
     2521 cggctgcgat ctcgtcggcc gatttctcgc gatagaacga cagatgcagc acggtgcgcg
     2581 cgcgcgtctc gagccgctgc agacagtgca gcaatgcttc gatgtcgagc tcgtcgtgcc
     2641 gcggcaacac ggactcgaca tcgagctcgg cgacgtcggt tggcaccgca cgcgtctcgg
     2701 cgtgacgcgc acgcgacgcg aggttgcgcg ccatgccgag cacgaaccga tcgaagcgct
     2761 cgggatcctc gacgcgaccc gcgcgcatcg cctcgatcat cgccacgagc accacctgga
     2821 cgagctcgcg tgcgcgatct tcgtcgcgta gatgcttgag gccatacagc cgcacgcgtg
     2881 gcgcgaatcg ccggcacagc tcggcctcgg ccgcatgatc gcgacccgcg gccgcaacac
     2941 gccgcacgag gtcagcatgg gtctccagcg tcaggtcggg cggcactcgt gcgcaaaata
     3001 gcacgcacct agacgccgag tgcgtcggcg agccctttga gcagcgcggc gccgttgccg
     3061 cggtacgagc tgccgtgcat cagtgcgagc gtcgtcggct cggtacgcgc gagcttctcg
     3121 aggatcgcgc gggtgttcgc ttcgatcgca acgccgccca tcgccgcgcg cattgcctcg
     3181 gacgggccga ggacttcttg ctcggtgatc ggcgcgacgt cggcgcccgc gtgcgtgaac
     3241 aggtcaccgc acaagagcgt gcggctcgtc gactcgaaca tgtagccgca ctcccagttg
     3301 tgcgggaggt tcggcgcgtc gagccagcgc accttgcgcg tgccgagtga cagctcttcg
     3361 ccgtcggcga gcggccgcgg cgcacggatc gcgtagtcct cgaccgacac catcgcgccg
     3421 atcatgctgc acaccgcctg cgactgcggc gccgcggcga gccagtcgtt gagcgagccg
     3481 cactcgtcag actcgaagtg cgagaacccg acgtaacgga gcttcgcgac gtcgccgagc
     3541 acgtgctcga ccgcttggcg cacgagcgga aacatcttgc ggaggccggt gtggaacagc
     3601 agcggcgcgt cgtcgacgat taagaactgg ttgaacgtga agccgcccgg cagcccgggg
     3661 ttcggcggca ccggcgtgct gatgcgatag atgctcggtg cgatctcgtg gacgttggtg
     3721 ccggactgcg aattcgtgac ggtcatcgtg gggctctcct ttcctcgatg tgtcgcgagc
     3781 ggcccaagcg ttacaaagaa atcgtcagga cgcgctatct gccgactcgg cctcgggttc
     3841 ttcggcgtcg tccttgtccg agatgatcct gcccgacacc cgctgaacgt tctcgcggac
     3901 gatcgtggtc atcatgcgct cgatcttctc ggggtccggg aacaggtccc agaccggcgc
     3961 ggcgtcatcg gcctgcggct cgcgcggaac atcgtggcgc gacgcccaat cgatgcgctg
     4021 ccgcggtgac ggatggctgt cgtagggcga cggctcccga ttcatcgcct tatcgacggc
     4081 aatcgcgagc tggtcgggag aagtcttggc ctcgggatcc cagtcgtaga gattcacgag
     4141 cgaccaatcc ttctcgagca cttcgttgac ctttgcgccc aggtcgccgt cgaactcgac
     4201 cgtgcgcgcg acgacgtggc gataacctgc gacgaatgcc gcgttgccgt acgcgcgaat
     4261 cgaccaccga tcagcgagga cttcctgcag ccgcgacgcc ccctgcgaga tgccgaggta
     4321 catgcgccag aatccgcgca agaaccacca gaccgggttg tagatcgtgt tcgcgccgga
     4381 cttcgccatt cgctcgacga cgctgaacag cgagcggcgc actgcgatcg cgaagccgcc
     4441 gccggcggtg tcctcgttgc ggaaatgtcc gtactcgtgc gcgagcaccg cccgcagttg
     4501 cagctgcgtc atgctgtcga actgcgcgac gccgaggatc agccggcgct cgctgggccg
     4561 acgcaagatc gtacgcaaca gtccggtgcg ctccatcacc gcgatatcgg tgcccggtac
     4621 gaggtgaacg acatcgaccg gacgcgtgcc gatcgcagcg gcaacttcgt cgagcattgc
     4681 gtgcagcttt ggatacgccg tcagcacgat ccgctcgctc cgcggatcgg gctcgcgtcg
     4741 caggaacaag ctgcgcacga cggcgatgat cgttccgaag atcatgacga cgaccaacac
     4801 gatcacgtag accggaatcg ccccgatctc ctcgaagaca taaagcgcga agaacccggc
     4861 cgcgatgacc gcgacgatca tgagcggcag cgacaggtag aagtacaagc ccgcaagccc
     4921 gaggacggcc ttgtagatct tgtggagccg gcgttcgcgt ggcgtgccgg tcgcatcgag
     4981 cgagtcgttg cggtcgacgg cgcgcagcgt ggcccgcgag agcgcattgc cgacaccgaa
     5041 cagcacgagc atgacgaggc cccacaagca gaacgtcagc aggcccggct cgagatactt
     5101 gtcgacatcg atgatcggtt cctgctgctt ctgctccgct tcctcgatct gcttgttcat
     5161 cccgtcgtag gtgtactgat cgagccccgc ctgcttcgcc ttctcgaggt acccgtgtgc
     5221 ctcgtcgaag tcacgctcgg ccaacgcgcc gcccacggca aagaagttcg cctgcacacc
     5281 gttcggatcg atcgcgagca ggtgtttgct gcatcgcgcg aagccgacgt cgttcttgct
     5341 ctgcaggccg gcgatacacc agatgatgtt cagcgtgacg tcgttcggct tggcctccgc
     5401 tgcctcgcgt gcaaggtccg tgtagcgccc gacgtcctcg gcgcgattgc gtctcatcaa
     5461 agccttcgcg agtgcgccct tgtcgtacgg cgagctcggc acgagcgaga gcgctttttc
     5521 gcattcgaca actgcgtcgt cgagctgagc cttcgcgaag agcacaccgc agaccctgcg
     5581 atgcgggtgg tccacgtttg acgcgagctc ggcggcgcga cgatacgccg caagtgcatc
     5641 gtcgacacgg tccgcatcac gtgccgcgtt gccttcgtcg tacgcggcag cggcctcggc
     5701 acttaccgcc gcaacgtccg cgcgaaactg cttctctgcc tcggggtcct tcggcaatgc
     5761 cgcgagcgaa gactgggctg atgcgcgacc aagcgtcagc atcagcacag cggcggcgag
     5821 gacgacgcgc aacatccgct cagtctatca agcctgctcg tgagctgacg ccgcaaactg
     5881 ctgcggcgtc gcgatgacgg cggcgctcag ctcccactcg ggatcatgat gttgcgcatg
     5941 tcggcatcga gcgtcgaatt gatgaattcg atcgtcttgc cgcgcaggcg cgtcttcgtc
     6001 gccatgaacg ccgag
//
LOCUS       DASUFX010000698         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1916892, whole genome shotgun sequence.
ACCESSION   DASUFX010000698 DASUFX010000000
VERSION     DASUFX010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916892"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFV99_28190"
     CDS             complement(<1..1247)
                     /locus_tag="VFV99_28190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5063302.1"
                     /translation="MTLDVKRYPILVVDDEQDNLDAFRFNFRKTFDILTATSGPEALT
                     ILENKDVAVVVTDQRMPKMTGVELLREVRERQPESVGIILTAFTDVDVLIEAINLGQV
                     YRYITKPWDAKEVRGVLQYAIERFHLQRENKKLAAQLAEYTGYLNQQLHGEFDFGNII
                     GDSAALREVLSKVEQVAPTSSTVLLRGETGTGKELVAHAIHINSPREEKPFVRVNCAS
                     LAPGVLESELFGHEKGSFTGAVERRRGRFELADGGTLFLDEVGDLPMEVQIKLLRTLQ
                     ERELERVGGNETIKVDVRVVSATNRNLEKMIEEGEFREDLYYRLNVFPINLPPLRDRL
                     EDLPILATHFIAKFARQMGVPNAGAAPDGLQKLREYNWPGNVRELENIVERAMILARG
                     APLGASHLDFGRRAVPTTAANQSG"
     gene            complement(1303..2304)
                     /locus_tag="VFV99_28195"
     CDS             complement(1303..2304)
                     /locus_tag="VFV99_28195"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5063303.1"
                     /translation="MAPERPSDIPKTWVIVLVLAAGIVMAPFVGWIVLAIWLSGFAHG
                     LHDRITRRLHGRVKLAALLTCVVLTLVLVPIGIVLTLLVMDAIALITQLAQSDRAHTL
                     LVSLVEPENGAAPSKSIGELVLSQGDRAIAFAKTILSSAAQIVIGLVILVAGIYSILV
                     DGKRWYAWAVENAPIGSHALRRMAAAFVETGRGLAFGVLGAGLLQALVATAAYLVLDV
                     PQALALGLLTLLFSIVPAIGTAIVWVPVATGLALTGRVGAGIGLAIYGVVVIGTIDNL
                     ARPWLARRGKLQLPTFVVLVSMFGAVELFGGWGVVFGPLLVRLAKEALELRHEAMPA"
BASE COUNT          437 a          822 c          770 g          403 t
ORIGIN      
        1 cccgactgat tcgccgcggt ggttggcacc gcgcgacgac cgaagtcgag gtgcgaggca
       61 ccgagcggcg cgccacgcgc gaggatcatc gcgcgctcga cgatgttctc gagctcgcgg
      121 acgttgccgg gccagttgta ctcgcgcagc ttctggagac cgtccggtgc cgcgccggcg
      181 ttgggcacac ccatctggcg cgcaaacttc gcaatgaagt gggtcgcgag gatcggcagg
      241 tcctcgaggc gatcgcgcag cggcggcaga ttgatcggga agacgttgag ccggtagtag
      301 aggtcctcgc ggaactcgcc ctcctcgatc atcttctcga ggttgcggtt cgtcgccgac
      361 acgacgcgga cgtcgacctt gatcgtctcg ttgccaccga cgcgctcgag ctcgcgctcc
      421 tgcagcgtgc gcagcagctt gatctggact tccatcggca ggtcgccgac ctcgtcgaga
      481 aacagtgtgc cgccgtcggc gagctcgaag cgaccgcgcc gccgctcgac cgcgccggtg
      541 aagctgccct tctcgtgacc gaacagctcg ctctcgagca cgcccggcgc gagcgacgcg
      601 cagttgacgc gaacgaacgg cttctcttcg cgcggcgagt tgatgtggat cgcgtgcgcg
      661 acgagctcct tgccggtacc ggtctcgccg cgcagcaaca ccgtcgagct cgtcggcgcg
      721 acttgctcga ccttcgacag cacttcgcgc agcgctgcgc tgtcgccgat gatgttgccg
      781 aagtcgaatt cgccgtgcag ctgctggttc aggtagccgg tgtactcggc gagctgcgcc
      841 gcgagcttct tgttctcgcg ctgcaggtgg aagcgctcga tcgcgtactg cagcacgccg
      901 cggacttcct tcgcatccca cggcttcgtg atgtagcgat agacctggcc gaggttgatc
      961 gcctcgatca gcacgtcgac gtcggtgaac gccgtgagga tgatgccgac cgactcgggc
     1021 tgccgctcgc gtacctcgcg gagcagctcc acaccggtca tcttcggcat gcgctggtcg
     1081 gtgacgacga ccgcgacgtc cttgttctcg aggatcgtca gcgcctcggg gccgctggtc
     1141 gccgtcagga tgtcgaacgt cttccgaaag ttgaatcgga aggcatcgag gttgtcctgc
     1201 tcgtcgtcga cgacgagaat cggatagcgc ttgacgtcga gggtcaccgc actcaacggg
     1261 tatcacacac cggcttcgcc ggaaaggata cgcgggaaag gttcacgccg gcatcgcctc
     1321 gtgacgcagc tcgagtgctt cttttgccag ccgcacgagg agtgggccaa agaccacgcc
     1381 ccagcctccg aaaagctcga ctgcgccgaa catggagacc agcacgacga acgtcggcaa
     1441 ctgcagtttg ccgcgccttg ctagccacgg ccgcgcgagg ttgtcgatcg taccgatcac
     1501 gacaacgccg tagattgcca ggccgatgcc ggcccctacg cgtccggtca acgcgaggcc
     1561 agttgcgacc ggcacccaca cgatcgccgt gccgatggcc ggcacgatcg agaagagcag
     1621 cgtgagcagg ccgagcgcca gcgcctgcgg gacgtccagc acgagatacg ccgccgtagc
     1681 gacgagtgct tgcaatagac ccgcaccgag gacgccgaac gcgaggccgc gcccggtctc
     1741 gacgaacgca gccgccatcc ggcgcagcgc gtgcgaaccg atcggtgcgt tctcgactgc
     1801 ccacgcgtac cagcgcttgc cgtcgacgag gatcgagtag atcccggcga ccagaatcac
     1861 gagcccgatc acgatctgcg ccgccgagct caagatcgtt ttcgcgaacg cgatcgcgcg
     1921 atcgccttgc gacaagacga gctcgccaat gctcttactc ggcgccgccc cgttctcggg
     1981 ctcgaccagg gaaacgagca gcgtgtgcgc gcgatcgctc tgtgcgagct gcgtgatcaa
     2041 cgcgatcgca tccatcacga gcagcgtcag gacgatgccg atcggaacca gcacgagcgt
     2101 gagcacgacg caggtcagca acgcagccag cttcacgcgt ccgtgaagcc gccgcgtgat
     2161 ccggtcgtgc agcccgtgtg cgaatccgct cagccagatc gcgagcacga tccagcccac
     2221 aaacggagcc atcacaatgc cggccgcgag aaccagcacg atcacccagg ttttcgggat
     2281 gtcacttggt cgctcgggcg ccacaccccc ttccaaaagc agcaatcgga ccagttccca
     2341 cccacatgta ggaaaatttg gaccgggccc cggaaccctc tagcattttc ggtcaatggc
     2401 ttcgtctcgg cgtccgcgca ggtattccca gg
//
LOCUS       DASUFX010000699         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_805757,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000699 DASUFX010000000
VERSION     DASUFX010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805757"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFV99_28200"
     CDS             complement(<1..264)
                     /locus_tag="VFV99_28200"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX5063304.1"
                     /translation="MTATLTYSGVSIEIANVLVDTGAASTIINADLAADAGVYVSPTD
                     TLRRLRGVGGHEHVFTRRVDRFAIGDRGLDNFGIRCTDHVLAST"
     gene            248..703
                     /locus_tag="VFV99_28205"
     CDS             248..703
                     /locus_tag="VFV99_28205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063305.1"
                     /translation="MSVAVTNARSCRNVSCTKPPNPQPQTLLELELRLTRVHEAELTV
                     RMRAAKLAVSLHRGSTVRADLDDPNTIEDAVVVRAVRFERFNDEPLVRMCRADLAPPH
                     AMTLAGCCDTGQFLAVAAEAAGRLRGSSSSVGHQDTFAAQAPLQEAEIV"
     gene            complement(770..1318)
                     /locus_tag="VFV99_28210"
     CDS             complement(770..1318)
                     /locus_tag="VFV99_28210"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX5063306.1"
                     /translation="MIDRRPGRVVVRVRGKGARALFEGEVGGHRWQRIPETEKRGRVH
                     SSTITVAVLDEAVATELVIPESDLEWFACRSPGKGGQNVQKTDSAVQVKHLPTGLFVR
                     SHEQRSYHQNKAVALARLRERLLAAQRRADTDARAAERRAQVGSGMRGDKRRTIRVQD
                     DQVIDHETGRTWRYRDYERGNW"
     gene            1612..2067
                     /locus_tag="VFV99_28215"
     CDS             1612..2067
                     /locus_tag="VFV99_28215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5063307.1"
                     /translation="MSVATKPPPKDWPRMSASVFYSEAAKAIDWLCNAFGFEVQLRVE
                     GEAGRIEHAELVLAGALITTGDVPRKDREWRRTPKEAGGCTISLCFYIDDVDAHCERA
                     RKAGAVIAQEPKTTDYGDDYWVDRSYGCFDPEGHHWWFMQRIKDNPRKS"
     gene            2064..2408
                     /locus_tag="VFV99_28220"
     CDS             2064..2408
                     /locus_tag="VFV99_28220"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5063308.1"
                     /translation="MTPNLDATFAALADPTRRGVIELLRREPRRASDLADALDASRPA
                     MSRHLRVLRASGLVETTEDDTDDADARERIYQLRKAPFEQLREWIEQTERFWGVQLDA
                     FKAHVERTRGKR"
     gene            2405..2899
                     /locus_tag="VFV99_28225"
     CDS             2405..2899
                     /locus_tag="VFV99_28225"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5063309.1"
                     /translation="MIEGDRATVTVLVDVEPAVAFEVFTAEIDQWWRHGVAYRAAGRN
                     PGKVMFECKLGGRLLEQYDGPAGPAIYEAGRITAWEPPARLAFEWRGGNFAPGEITLV
                     EVTFTRAESGRTQVVLVHSGFAALRPDHPVRHGEPPAQFIGRVGQWWGKLLTSLREHA
                     SDRT"
     gene            2914..>3591
                     /locus_tag="VFV99_28230"
     CDS             2914..>3591
                     /locus_tag="VFV99_28230"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5063310.1"
                     /translation="MGSAATNVTAAAAITSINPATGEVLGEVPVMTAEQVRAAVETAR
                     AAQREWARVPVEARCRRVAKFADVLMARADEVIEVLVREGGKTRQEALGMELVVVADL
                     VRYFAKHAPEMLAPEPIALHLMKHRASYLHFTPRGIVGVIAPWNFPFSIPVGETMMAL
                     IAGNGVVLKPSEVTPLIALKARELYLAADLPPELFQIVTGRGPTGAALIDSGIDYCVF
                     TGSVATGK"
BASE COUNT          613 a         1192 c         1178 g          608 t
ORIGIN      
        1 tgtcgacgca aggacgtggt ccgtgcaccg gataccgaag ttgtccagac cgcgatcacc
       61 gatcgcgaaa cgatcgacgc gtctcgtgaa cacgtgttcg tgtccaccga cgccacgaag
      121 gcggcggagc gtgtccgttg ggctgacgta gacgccggca tcagccgcca ggtcggcgtt
      181 gatgatcgtg ctcgcggcgc ccgtatcgac cagcacgttc gcgatctcga tcgacacgcc
      241 cgaatacgtg agcgtcgccg tcacgaacgc gaggtcatgc cgaaacgtca gttgcacgaa
      301 gcccccgaat ccccaaccac agacgctcct cgaactcgag ctccggctta ctcgtgtgca
      361 cgaagcagaa ctcacggtgc ggatgcgcgc ggcgaagctc gccgtatcgc ttcatcgcgg
      421 ctcgaccgtc cgagcagacc tcgatgatcc caacacgatc gaagacgcgg tggtcgttcg
      481 cgctgtgcgc ttcgagcgct tcaacgacga gccactggtc aggatgtgcc gcgcggatct
      541 cgctccaccg catgcgatga cgctagccgg ctgctgtgac accggccagt tcttggccgt
      601 cgccgcggag gcggcgggta gattgcgcgg ttcctcgtcg agcgttgggc accaagatac
      661 gtttgctgcc caagcgccgc tgcaagaggc cgagattgtt tgaattttgg tggaggcggc
      721 gggagtcgaa cccgccgccg aagaagctgt aggcggtggg ttcgatcgat taccagttgc
      781 cgcgctcgta atcgcggtag cgccacgtcc gcccggtctc gtgatcgatg acctggtcat
      841 cttgcacgcg aatcgttcgg cgcttgtcgc cgcgcatccc actgccgacc tgcgcacgcc
      901 gttcggcggc gcgcgcatcc gtgtcggccc gtcgctgtgc cgcgagcagc cgctcgcgca
      961 gccgcgccaa cgccaccgcc ttgttctggt ggtagctgcg ctgctcgtga ctacgcacga
     1021 ataggcccgt cggcagatgc ttcacctgca cggcgctgtc ggtcttctgc acgttctggc
     1081 cgcctttgcc cggcgagcga cacgcgaacc actccagatc gctctcgggg atcacgagct
     1141 cggtagcgac ggcttcgtcg aggaccgcga ccgtgatcgt gctcgagtgt acgcggccgc
     1201 gcttttccgt ctcgggtatg cgttgccacc tgtggccccc gacttcgccc tcgaacagtg
     1261 cacgcgcacc cttgccgcgt acgcgcacga cgacgcgccc cggccggcga tcgatcacct
     1321 cgaggctaaa gacacctccg gcctccgagc ttgccgtaga tggcgagctg ctcttcaacc
     1381 agtagctttg cgtccgcacc accttcggca gcgcggatct cgacgattac caatgacgta
     1441 tccatgactc tctccttgct caaaacacaa aacgattccg ttccgaacga cccaaacgtc
     1501 gtcggcggct acgtctcgtg gagcgggagc agaaacatca caaccttctc ttagcacgct
     1561 ctaacgcaca tggctcttga aaatggttaa cgaacgagtt aatcaatgtg catgagcgtc
     1621 gcaaccaagc cacctcccaa ggattggccc cggatgtccg cgtcggtgtt ctacagcgag
     1681 gctgccaagg ccatcgattg gttgtgcaac gccttcggct tcgaagttca gctcagggtc
     1741 gaaggagaag ccggtcgcat cgagcacgcc gagctcgtgc ttgctggcgc cttgatcacg
     1801 acgggcgacg taccgcgcaa ggatcgcgag tggcgccgga caccgaagga ggcgggcggc
     1861 tgtacgatct cgctctgctt ctatatcgat gacgtcgacg cgcattgcga gcgcgctcgc
     1921 aaggccggcg cggtgatcgc ccaggaaccg aagacgactg actacggcga cgattactgg
     1981 gtcgaccgca gctacggctg cttcgatccc gagggccacc actggtggtt catgcaacgc
     2041 atcaaggaca acccacgcaa gtcgtgacgc cgaacctcga cgccacgttt gccgcgctcg
     2101 cggatcccac acgccgtggg gtgatcgagc tgttgcgtcg cgagccgcgc cgcgcgagcg
     2161 atcttgcgga cgcgctcgac gcgagccgac cggcgatgag ccgccatctg cgcgtgttgc
     2221 gcgcgagcgg cctcgtcgaa accaccgaag atgacaccga cgacgccgac gcgcgcgaac
     2281 gcatctatca gctacgcaag gcgccgttcg aacagctgcg cgagtggatc gagcagaccg
     2341 agcggttctg gggcgtccag ctcgacgcat tcaaggcgca tgtcgaacgc acgcgaggca
     2401 agcgatgatc gaaggcgatc gcgcgaccgt gaccgtgctc gtcgacgtcg agccggccgt
     2461 cgcgttcgag gtgttcaccg cggagatcga tcagtggtgg cggcatggcg tcgcgtatcg
     2521 cgccgcgggt cgcaacccgg gcaaggtgat gttcgagtgc aagctcggcg ggcgcctgct
     2581 cgagcagtac gatggcccgg ccggccccgc catttacgag gccgggcgga tcacggcgtg
     2641 ggagccgccg gcgcgactcg cgttcgaatg gcgcggcggg aacttcgcgc cgggcgagat
     2701 cacgctggtc gaggtgacgt tcactcgcgc ggagagcggg cgcacgcagg tggtgctcgt
     2761 ccactctggc ttcgccgcgc tgcgcccgga tcatccggtg cgtcacggcg agccgcctgc
     2821 acagttcatc ggcagggtcg gccagtggtg gggcaagctg ctcacgtcac tgcgcgagca
     2881 cgccagcgat cgcacgtagc gtacaatcgc ggcatgggct ccgccgcgac gaacgtcacc
     2941 gctgccgccg cgatcacgag catcaacccg gcgaccggcg aggtgctcgg cgaggtgccg
     3001 gtgatgaccg ccgagcaggt gcgcgctgcc gtcgagactg cacgcgccgc gcagcgcgag
     3061 tgggcgaggg tgccggtcga ggcgaggtgc cggcgcgtgg cgaagttcgc cgacgtgcta
     3121 atggcccgcg ccgacgaagt gatcgaggtg ctcgtacgcg agggcggcaa gacgcgccag
     3181 gaagcgctcg gcatggagct cgtcgtcgtc gccgatctcg tgcgctactt cgcgaagcat
     3241 gcgcccgaga tgctcgcgcc cgagccgatc gcgttgcatc tgatgaagca ccgcgcgagc
     3301 tacttgcact tcacgccgcg cggcatcgtc ggcgtgatcg cgccgtggaa cttcccgttc
     3361 tcgattccgg tcggcgagac gatgatggcg ctgatcgccg gtaacggcgt cgtgctcaag
     3421 ccatccgagg tcacgccgct gatcgcgctc aaggcgcgcg agctctatct cgccgccgac
     3481 ctgccgcccg agttgttcca gatcgtgacc ggacgtggcc cgaccggcgc cgcgctgatc
     3541 gacagcggca tcgattactg cgtgttcacc ggttcggtcg cgaccggcaa g
//
LOCUS       DASUFX010000700        27377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1916960, whole genome shotgun sequence.
ACCESSION   DASUFX010000700 DASUFX010000000
VERSION     DASUFX010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916960"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFV99_28235"
     CDS             complement(<1..317)
                     /locus_tag="VFV99_28235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063311.1"
                     /translation="MRPAVIPKLSELPTERERRNAILDSSHAEPGPENRPTSKKARKA
                     EAAAATAAAVIGVLFSNHANVTLGGAATIDENELFEDTKKHKPAQGEAGGDEEKETDP
                     PPEP"
     gene            complement(456..995)
                     /locus_tag="VFV99_28240"
     CDS             complement(456..995)
                     /locus_tag="VFV99_28240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063312.1"
                     /translation="MSDEFYLHEDEWGMIELLPRENYAERQKMVDAAAAHAEAHRAPG
                     GAGWTAMYVAPEAKTPISERGITLTALAEALGPGWKRSARVTSGYSTYVEEATASYAF
                     RPPQRHHNWNVIYGNVQGELVTSLCVTHCEPPIMPELHRLGTTFRLMLCDLWTDVVID
                     LADPVALARYVSPDDSDDE"
     gene            1026..2648
                     /locus_tag="VFV99_28245"
     CDS             1026..2648
                     /locus_tag="VFV99_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5063313.1"
                     /translation="MAPVKKPETPQEYKTMSGIPLKVTYKPEDLSSRGWTYEEKLGDA
                     GQYPFTRGPHQTMYRGKPWTMRMFAGFGTPEDTNQRFKFLLANGQTGLSTAFDMPTLM
                     GYDPDHPRSLGEVGREGVSVATLDDMMRLFGDIPLDKVSTSMTINAPAAVLLALYVAV
                     AEKQGVSPDKLRGTLQNDMLKEFIAQKEWISPIRSHMRIIRDMLVWCTRTMPQWNTIS
                     VSGYHIREAGATGVQELAFTIADGIGYCQLGIEGGLDIDEFGPRLSYFWDVHNDFFEE
                     IAKFRAGRRMWAKIMRERFGAKNPKSWLLRTHAQTAGVSLMAQQPLNNVVRTTVQALA
                     AVLGGTQSLHTNSYDETYALPTEDAAELALRTQQLIYEETGVANVVDPLAGSYFIETL
                     TDQVEAAAWEYINKIDAMGGIVKAVEEGYPQREIARAAFEFQREVDSGRRTIVGVNKY
                     TTPGEGDHIPTLKIELEIEHSQIEKVKNFRGQRDEAVVQRALAAVKAALKDDKQNVMP
                     PILEAVKVNATLGEICDLFRSELGEYRDPAYL"
     gene            complement(2645..3277)
                     /locus_tag="VFV99_28250"
     CDS             complement(2645..3277)
                     /locus_tag="VFV99_28250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063314.1"
                     /translation="MRALLVFLFACRTVEPSPLIDGSTPDTPFDVDSNDKSAACASTF
                     GTALTNAFGRLDGTILAVVMPGNNRCAMPNSTHVVLQVTTDGAAYRMVINVLSTSPDP
                     HVWLDDINAPLAGDPWAEGWHPGVQLDYVSTLGVGRSSFTEANQLEAIKRLDDAFVIG
                     AHVSVFATSSGGASAHLVHRNVTNADGAIVIDPESASPRYLLSAFPEQQF"
     gene            complement(3318..6281)
                     /locus_tag="VFV99_28255"
     CDS             complement(3318..6281)
                     /locus_tag="VFV99_28255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063315.1"
                     /translation="MMRKLGLLCLFLAMPACTDESTEDPSELGGNKLPASSDPHTLFE
                     SLQTRPVALSPNGKMLFAANTPDNRLEIYSVGSGGLVSKGSIAVGLEPVAVAARNDSE
                     VWVVNHVSDSVSVVSVSSSGVATVTRTLLVGDEPRDIVFAGTNRDRAFITTAHRGQNT
                     GDAYDLQTPGVGRADVWVFDANNLGSAPGGNRLTKLTLFADTPRALAVSGDGRTVYAA
                     AFLSGNQTTSVSEYAVATVYPNGMPGPQTVTVNGVTYPQPRTGLIVKFVNGHWVDAYG
                     TVFDAFVKIKLPDNDVFAIDATANPPVATRTYAHVGTTLFNMAVNPQSGKVYVTNTEA
                     HNDVRFEGHTPGFSSVVGHIADTRITVIDPTSGAVSPRDLNPHVDYTTDGSASERALS
                     VAFAQDIAVSANGSTIYTVAQGSSKLAIYRTADIESGVASPTLTSQVTLSGGGPTGVA
                     LDEARGRAYVLTRFDNSIKIVDLAMRKEIGTVPMFNPEPESVVDGRKYLYDANLTSGH
                     GVAACASCHIGGDKDDLAWDLGNPGGGPLPIPTLGNVFALPPAAIIQLLPYTEPEFAA
                     NNPLKGPMTTQSLRGMDNHGAMHWRGDRNGAVQQSGAPFLDGNGQPITSAQPNAGLFD
                     EFKAFTSFNVAFPGLVGRADQLSEHDMGEFATFILQVMYPPNPVRNLDNSLTAEQQQG
                     RDFYFNHAGTQELPSDRFHNCNGCHTLDLAGNSGSSPHPGFFGTDGRLSFEFETQIFK
                     VPHLRNMYTKIGMFGSSPDSIQPGTIVLQGPNVPVVDQVRGFGFQHDGSLGQLEHFFT
                     GLVFIRATENILLAGGIVVPPNPFGIPFVDVNKLNQGQVVFDGDGGFALRKAIVAFMI
                     AFDSNFAPVVGQQITVTKTNGSTAGARLDLLEARANAGECDLVGKSTSTAGPNLGALY
                     KAGSWQLASVFVPPVSDATLRSRIAQGIIPPVTFTCVPKGEGQRLAIDRDGDGYADWD
                     EVAAHKDPADSSSHP"
     gene            complement(6526..7608)
                     /locus_tag="VFV99_28260"
     CDS             complement(6526..7608)
                     /locus_tag="VFV99_28260"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063316.1"
                     /translation="MGDPQAPFAKVLEVLACHGVLDGDRIAPDVVLISIGDHFDYDFR
                     DPVGAAQDGLRLLRWLASHPSHQVTLLFGNHDASRVMELAHLADERFEAARLLARSID
                     DTEHTAGEAAAEQRTRDEYVPAFPELPPPGVLGRDYASFTTEQRALVQELLLAERFHL
                     ACVGELPDGRTALITHAGVTSRELGMLGKGGAPPAEIAAALEAQLHAAVDAVRGDWQS
                     GANTPLSLAPLHVSGGNGEEGGGLLYHRPSNPERPDADKAWALSAARPRRFDPRTLPV
                     GITQIAGHSGHAKCVHELGPWVTESAKTRKHGGIRTLRVRGNDVVYDMGIAPRADTVA
                     DLILIDGELRRVPATEVALLQLARVL"
     gene            complement(7655..8674)
                     /locus_tag="VFV99_28265"
     CDS             complement(7655..8674)
                     /locus_tag="VFV99_28265"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5063317.1"
                     /translation="MGRFRASTMFNLVIFAIGAVAFGFVVAHLGWTAIRNVIVSTGVW
                     FAVIAAIDLVSALCDAFAIYGFLRPKQRVSYWRVYAAQLSGMAINRLTPGNSLGEPVK
                     VTMLVRTVPVDLAVSAVVMFNLTTMYVGIAAIVLGVPLTALLLDLPRQVALLVWVGLA
                     VLVAFAIIVALFVRRGVVGTLIDGLAAFSIISPARAERWRAKVATIDARLRSIMTARD
                     SGIGRGLAGVLGSRVLNWVGTIVVLYAADIPMTAPLVVASLSVGILITWISNIIPLGL
                     GIADGTNYVLYGLLGAAPVAGLLFTLLNRLRTVVLALLGLTTMVITNTLHRRTAGVSA
                     ASATD"
     gene            8815..9171
                     /locus_tag="VFV99_28270"
     CDS             8815..9171
                     /locus_tag="VFV99_28270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063318.1"
                     /translation="MKRRPPHPLEHHPSFRAVIAAAPPVAELARELSDDGRELVGALA
                     IVCGDLAQGFASPPGSRARNEAHHRAWTTVREIDRAVTAARIHRRAPARLVAKAQRAI
                     DRADVMIAALPGVLPG"
     gene            9258..10196
                     /locus_tag="VFV99_28275"
     CDS             9258..10196
                     /locus_tag="VFV99_28275"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX5063319.1"
                     /translation="MRIHHAAVLLFAAVLATGCKKRGTGSGGGGGGGGGWLIGSSGLM
                     VNINDDALVGEYDLGSEANLNAIACRYQAEAWVVGDDATVLYTNDGGTSWVSQTVPTT
                     AHLRTLATQDDGPVFLGGDGTFLVTSDNGLTWTQLGDGVTSFRSMSAAQAGTGVLALA
                     DDGGLWSYDGGTLTHRSTIAGARAVHQTPDGSIVMTAGSGIARSLDGGVTWRPLTVDP
                     SIVFDDLRVNEDGTAVAVGANGAIANVALNGTVSLQYVGDSTLHTIHIHDDEMGYAGG
                     DNGQVLITEDSGLTWRIGPNVGRTVRGVDGIGFNHR"
     gene            10349..11995
                     /locus_tag="VFV99_28280"
     CDS             10349..11995
                     /locus_tag="VFV99_28280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5063320.1"
                     /translation="MVVAAGSAHAAHAQPVEPEGGASADAAPAPTTSQPSPLAIDQLT
                     LSDDLIAAVGLRPIGDRRVEARVSTGNDATHQAALRATDTILGAGLELTGSALRSDVL
                     GDQSEAAARIAHTSDHHVAGLHATYTADHVGFGRQSFLTDMRTATYGAAWTESRAPGR
                     VTLELYGEQQTLRDTRQVGTLELDGSLLGAHVGFTSRSVQAFGLDHEFSGDIDVMQAN
                     GVASETDGDPDEMHMTMLPRTRHGRHRFLSAYIHDTVRVIESLDVHGGFVFEHWRWLT
                     NIASIYGPDLGESMDQDAAVISSLMIGPKVGALYRVTPNVAVTANAYRTLRTPTWQQL
                     MRPVQNGEVVTVADDQLHPETVTGAQLGPTVVTPKLEARVVGFWNEVAAPIADIDVGP
                     AARETTNLGHAREIGVDASASWRIAKSVLAGAGYTYTNARITDAASHPALAGNVRAQT
                     PRHRATAALTYDRPTILTVTGTVRFADQRFANAGNTIALAPVAVVDAMAARKLTHGLA
                     GFVSVENIFDRRFVWNQAGVDTIGAPRLVQVGVRLDSARW"
     gene            complement(12319..13785)
                     /locus_tag="VFV99_28285"
     CDS             complement(12319..13785)
                     /locus_tag="VFV99_28285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5063321.1"
                     /translation="MRATISLCVLLAAATATAAPNAAPFDKSGITDWTKAPAAAKEPA
                     FKPPVAKRSKLKNGMSLMLVENHALPIVSMVLIAPGAGAAADPDSKAGLASFTADLLD
                     EGAGGLSAIAIAEEQDRLGAGIGAGVDVDFAQVSVSTLTKTLDPTIDLLTKILTQPAF
                     EQKEFDRVKGDRSTSLELRRDRPREVAGLMLNAALYGLSSAYGHPMTGLRETFKDLSV
                     ADVQTFYKEHWNPAVMTLIIVGDFDAKALKTKLDATLGAWKPAGVKKPAKVAVKPEKI
                     AKRLLLVDRKDAAQSDVRIGLVGIDRKDKRYFQFEVLSSTLGGGFTSRLVQRLREQMG
                     ITYGARSGEDYRLSPGPFVISTAIVTPQTGAGLAEIFKMLDNLATVDVPAEELEKSKQ
                     NLIRALPSMFDTNASTAGAFADIAIFGLPDNWYASYAANVRKVTAKDVKTVAKTVIPS
                     KSMVISIVGDLSKIKPDVDKLDLGEAEMHDLYGMPIKQ"
     gene            complement(13785..15116)
                     /locus_tag="VFV99_28290"
     CDS             complement(13785..15116)
                     /locus_tag="VFV99_28290"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5063322.1"
                     /translation="MRRLTLLFLVALAVPAYADEPKITYQKFDLANGMRVYVIEDHKA
                     PTAYGVTWFRVGSKDEVANRTGFAHLFEHLMFKGSAHLPDGLMDKLLEAAGGWSNAFT
                     SSDMTVYQNVAASNFLEQMLWIDADRLAGLLDTFDKPKLDNQRDVVLNERRQSYENQP
                     YGMAEIILHENLWPKEHGYHWSTIGYPADLKAASVPDVADFFKKYYLPNNATMVIAGD
                     VKFDNVKKLVEKYFSWIPKGTDPQRPQYKTPPPITKEIVVDTTDDVQVPRVYLSWRGP
                     AHYTQDEPALVMASAILADGKSSRLYKRLVYDEKIAQDVRAYFEGETLGGEFRIVATA
                     KPGVDPKRLVKEISEEVAKLSTTAPEAKELERAQNSHEAGFLNGLEPTLQRAILLANY
                     DVFTNDPDYFAKDLARYRAVTPDQIKGVSAKYLKPTARVQLTIRPGKKVVK"
     gene            15250..16356
                     /locus_tag="VFV99_28295"
     CDS             15250..16356
                     /locus_tag="VFV99_28295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063323.1"
                     /translation="MRSGISDSTVERFHAFLGEEDQGRARAIGALDAFFAAASAALLL
                     LDRDLHCVQANERLSRLTRLPLEEHRDRPVRELVPVALESKLREILQSGEPLDKEPIE
                     LDGRSLLGTFFPVFDANHNISGLGGILIDYTEHKRLEAELRSAIEIRERVLAVVSHDL
                     RNPLGTIELAVSTLPESARDDREAARRIEIIERATKLMETLIGDLLDMATIQTGTLAL
                     HFADENADAVVREAIELHHPLAEEAHLRLVDETHLEGVRVRCDKARVLQVLGNLIGNA
                     IKFCKRGDAITVRGYADGRSLVLEVVDTGPGIPAADIPHLFEQYWSTARGRQRGTGLG
                     LFISHAIVAAHGGELTVASTLGVGTSFRIVLPLG"
     gene            16460..17035
                     /locus_tag="VFV99_28300"
     CDS             16460..17035
                     /locus_tag="VFV99_28300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX5063324.1"
                     /translation="MERPSQTLVRLELETHAYHRDADQPWLDLLDPFLTRLAYAEQLG
                     RMYCFECPVESALAYTPHVTSLIGHRARSRLLAHDLFALDYSQARLSPRLIAPFPTVA
                     SALGWLYVLERSARLYDLVVRNVAARLPGAPTEYVADPAAPARWTALGEVLDRVARTA
                     PIADQIVLAAHDAFRSLLDWYVGEQALRRGA"
     gene            17125..18756
                     /locus_tag="VFV99_28305"
     CDS             17125..18756
                     /locus_tag="VFV99_28305"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5063325.1"
                     /translation="MQAAVLAAAPDRDLLVSAQTGSGKTVAFGLALAPRLLGDAERFE
                     KAGAPRALIVTPTRELAQQVHRELQWLYGETGARLTTCVGGMDPRRETHALQHGVHIV
                     VGTPGRLCDHLERGALVLARIDAVVLDEADEMLDMGFREDLERLLDAAPTERRTLMFS
                     ATIPKPIATLAKRYQKGALRIAATAEGDRHRDIEYHAMPIIPRERDLAVVNLLRYHDA
                     PGALVFCATRDGVTHLAANLSERGFSVAALSGELSQRERNQALQALRDGRARVCVATD
                     VAARGLDLPDLGLVIHADLPQNAQVLVHRSGRTGRAGKHGLAILIVPDNARRYVERML
                     ATAHLDAKWRLPPSADDIRVRDQDQLVLELRALADEPSEDDRRVAQTLLTERSAEALV
                     AALVRSRRDARPAPEELTMPPSMRPKRPEPQRPQRVDRRKEQVPFEGPERRGAKPQRP
                     QPERPRGNGERRGDAVWFTINVGRSKNADPKWLIPLLCRRGNVTKRSIGKIQILARET
                     RVEIAADTAEQFAEAVRRPDPKDPRIHIERVDAEI"
     gene            complement(18753..19928)
                     /locus_tag="VFV99_28310"
     CDS             complement(18753..19928)
                     /locus_tag="VFV99_28310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063326.1"
                     /translation="MADMPKPPLVLYVDDERANRIVFEQSLKSEFTIKTVEDAKSALE
                     ALDREDVAVLVSDIRMPEVDGLELLRRAKERHPNTIRMVITAYSDVDPILRAINEGLV
                     ARYIVKPWDREELVQVLRWATELWNFGKDSAQLLRRLLETERLASLGGLTALYVHDLK
                     TPIMVVNNLLDELHAVADAVPTLHAAIEASDIDDRLKARLLQRVDDTPEIVQDAKQAS
                     TLIKDMIGGLSDYIRNKGPQETPVIDPTPVIQFTVNMFQRITVENRTAQIGYRGSKSL
                     PHVRISAVDLTQVLVNLVNNATQAVHARGEPNRHVALEARTQGDMLELQVRDDGIGMS
                     PEVLRKIGTPWFSTRPEGTGLGIANCQRLIGRAGGRMRIESEPGVGTTVTILLPTAA"
     gene            complement(20022..22148)
                     /locus_tag="VFV99_28315"
     CDS             complement(20022..22148)
                     /locus_tag="VFV99_28315"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5063327.1"
                     /translation="MWESRKRLRTVGAPLRVLVVDDDDIQLRALQRALRDDRSIELLV
                     ASNAIDGLLIIGNALPDLVIMDVYMPGLDGVETCRRIKANPATRDIDVILASVAMTPE
                     LELVAKAAGAERAVAKPYDLKELARPPQPSVAPPEPVAPSTRGADLLVSMLASAGVDV
                     VFGLPGGAISPVHDALLDNDIRVITTKHEAGAMFAAAGYAHTTGKLGVVAVTSGPGAL
                     NAMTGLASAWCDSLPVLLLVGDVPRGAQGRGVLQDSSSHGLQIVEMSRHISKMAAEIA
                     SPSALPHVLRRAIATALSGRKGPVVLTLPIDIMTANVTTPRSGGSVTMTNLVAAETID
                     EVAELLIHAERPLILAGSGVRGGGAPTRLRAVAERYSCPVATTPKGKGVFPETHPLSL
                     GVLGLGGHRSSRRYLDNNVDVVLAIGTSLGDVSTDGFAPALQASRALIHVDIDARQLG
                     KSYAPTHAIVASASELLGGLADRREEPKVIERPRTNGVERYHISSSTRLDRIAPQDAI
                     VELQRMLPRDTIYTVDSGEHFLYAVQYLETRDPDAFVVMTGLGSMGQSIGAAIGAQLA
                     HPGRSVAAICGDGCFAMNAFEIATAVQEQLPIRVFVFNDERLGMVENGHQNVYGRRPR
                     YSTGPLDICSIARGLGAVTMRVDGKHQFDVRLLSAPGPVVIDVRIDPDIMMPKVDRVA
                     VMATKQKAPPPAPAEPSAWPRLRVVN"
     gene            22339..24078
                     /locus_tag="VFV99_28320"
     CDS             22339..24078
                     /locus_tag="VFV99_28320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5063328.1"
                     /translation="MGVSFAVHERDRIGLIGANGAGKSTLLKMMVHGASGTSDPTLEP
                     DDGMITWRRDLVLEYVAQEPQLALDETVATALTREEVPDHEMHTVAAALQLPPLTSRV
                     GQLSIGERRRVALAHALLGKPDVLALDEPTNHLDARTIEWLETRLGAWPGALIVVTHD
                     RYFLDRVATRIVEIDRGKAYAYEGDYEDFLLAQADRLAIEAEAEYQRASFVRREIDWI
                     RRSPEARRTKAKARIDRFDAAVAAAPALEDKRPATARLTLPSGPRLGSSIVELKNVSR
                     TLGGKVLFKDLSLVMKPGDRIGIVGPNGAGKTTLIKTILGLEPPDSGAVVLGVNTKPA
                     YFEQGRSELRDELTVLEEVGDGYDWVELPDGKIHVRSFLRMLAFPENIADTKIGQLSG
                     GERNRVQLARLLRRGGNLLVLDEPTNDLDLPTLGALEEGLLSFGGCALIVSHDRWFLD
                     RVATAILAFEGNGNVTLWEGSYTFYAERRAASAKPQRQDKPDKDKPKPTRAAPPRKLT
                     FKERHELAGMEAAITAAETKVTELEATLSDPSVFKDRPTEVQTLVGDLDAARAEVERL
                     FARWQELEAIPKE"
     gene            complement(24087..24452)
                     /locus_tag="VFV99_28325"
     CDS             complement(24087..24452)
                     /locus_tag="VFV99_28325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5063329.1"
                     /translation="MRQRKILIVDDQPELARAIRRMLADHDVTAVASAREVLDRIEGG
                     ERFDVILCDLMMPEISGMELHARLQTTAPEQVDRMVFMTGGAFTSQAKSFFEQVANPT
                     IEKPFDRVALFAVIDELIR"
     gene            complement(24573..25130)
                     /locus_tag="VFV99_28330"
     CDS             complement(24573..25130)
                     /locus_tag="VFV99_28330"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5063330.1"
                     /translation="MQPFVCEGEEVMQHNMTKHEHWIAKVCDQLNKLIQLDFDAAGTY
                     REAILHIDDPAARSDLHAFLGDHERHVMELSQIVRDLGGTPIEAHRDLKGGILEGMTR
                     LRSRGTLGALRAMRMNEKLTNRTYDRASEIYMPPIGQAIVVENLTDERRHLAAIEAHI
                     DRLSGKHFYDIDRVDREATEGPFVR"
     gene            25262..>27377
                     /locus_tag="VFV99_28335"
     CDS             25262..>27377
                     /locus_tag="VFV99_28335"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5063331.1"
                     /translation="MQRRTFLLSAASSAVLLSFKSKLSFAKGGEKMPSEPLLAPWTGP
                     YGGVPPFNKIQVKDFKPALKKGMDLSRADIKAIAESKDAATFENTIAALEDSGRAFSR
                     CGSMFGTFTSTMNSKPMQTVEKEMAPVIAAFSDEITQNEALFARIKTVYDGRAKAKLT
                     PEQNRLVEVTYQNFARKGASLGAKEKARLKEINQKLATLFTSFSQNVLADEEGQEIVL
                     DKEADLAGLPESLRASMKTAAEGKKLKGKWLISNTRSSAEPFLTYSSRRDLREKVFRM
                     WSSRGESADHNNLPVMTEILALRGERAKLLGFKTHAHWIIDDNMAKTPDAAMALMMKV
                     WKAATARVKEEVADMQKVADAEKAGHKIEAWDYRYYAEKVRKAKYDLDQDEVKAYLQL
                     DKLRDAMFWAAGQLYGLEFKPIKGVPVYHKDVTTYEVVRGGKHVGLWYFDPYARAGKR
                     SGAWMSEYRTQEGFKTAVAPIVSNNSNFVKGKAGTPVLISWDDATTMFHEFGHALHGL
                     NSEVNYPTLAGTNTKRDFVEFPSQLNEHWLTTPEVLNKFCVHYQTGKPMPPELVAKIE
                     KAKNFNQGFKTVEYLAAAIYDLRIHMTPTDKPIDPAKFEQTTMKEIGCPTEIVMRHRP
                     PAFLHIFSDDAYSAGYYVYIWADTLTADAAEAFVEAKSFYDKATAKKLHDNIMSVGNS
                     IPPDVAFRQFRGRDVDTDALMRD"
BASE COUNT         4807 a         9059 c         8952 g         4559 t
ORIGIN      
        1 ggctccggcg gcgggtctgt ttccttttcc tcgtcgccgc ccgcctcgcc ctgcgccggc
       61 ttgtgcttct tcgtgtcctc gaacagctcg ttctcgtcga tcgtcgccgc gccgccgagc
      121 gtgacgttcg cgtgattcga gaacaatacg ccgatcaccg ccgcggccgt cgccgccgct
      181 gcctccgcct tgcgcgcctt cttcgacgtc gggcgattct ccgggccggg ctcggcgtgc
      241 gagctgtcga ggatcgcgtt acggcgctcg cgctcggtcg gtaattcaga cagcttggga
      301 atcacggctg gtcgcatcgg accagcgcac gcagcacaaa ggacgaagac ggcggctcgc
      361 acgggccacg cgccgcagca tttctcgtgc ccggattcgc gccgcgaatt ctcatgcgtt
      421 ggcggcgcac ccggcgtggt gctcgcgcca cacagttact cgtcgtcact gtcgtcgggc
      481 gagacgtacc gtgcgagcgc aaccggatcg gcgagatcga tcacgacgtc tgtccacaga
      541 tcgcacagca tgagccggaa cgtcgtgccg agccgatgca gctccggcat gatcggcggc
      601 tcgcagtgcg tgacgcacag gctcgtcacc agctcgccct gcacgttgcc gtaaatgacg
      661 ttccagttgt ggtggcgctg gggcgggcga aatgcgtacg acgccgtggc ttcttcgacg
      721 tacgtgctgt agccgctggt cacgcgcgcg ctgcgcttcc agccgggccc gagtgcctcc
      781 gcgagcgcgg tgagcgtgat gccgcgctcc gaaatcggtg tcttcgcttc aggcgcgacg
      841 tacatcgcgg tccagccagc gccgccgggc gcgcggtgtg cctcggcgtg cgcagcggct
      901 gcgtcgacca tcttctgacg ctccgcgtag ttctcccgag gtagcagctc gatcattccc
      961 cactcatctt cgtggagata gaactcgtcg ctcatctgag gtggatccca tggtagatga
     1021 acgccatggc cccggtgaag aagcccgaga ccccgcagga gtacaagacc atgagcggta
     1081 ttccgctcaa ggtcacctac aaacccgaag acctctcatc gcgcggctgg acgtacgaag
     1141 agaagctcgg cgacgcgggc cagtatccgt tcacgcgcgg accgcaccag acgatgtacc
     1201 gcggcaagcc gtggacgatg cgcatgttcg ccggcttcgg tacgcccgaa gacacgaacc
     1261 agcgcttcaa gttcttgctc gcgaacggcc agaccggtct gtcgactgcg ttcgacatgc
     1321 cgacgctgat gggctacgac cccgatcacc cgcgctcgct cggcgaagtc ggtcgcgaag
     1381 gtgtgtcggt cgcgacgctc gacgacatga tgcggctgtt cggtgacatc ccgctcgaca
     1441 aggtgtcgac gtcgatgacg atcaacgcgc cggcggccgt gctgctcgcg ctctacgtcg
     1501 cggtcgccga gaagcaaggc gtgtcgcccg acaagctgcg cggcacgctg cagaacgaca
     1561 tgctcaaaga gttcatcgcg cagaaggagt ggatcagccc gatccgcagc cacatgcgaa
     1621 tcatccgcga catgctcgtg tggtgcacgc ggacgatgcc gcagtggaac acgatctcgg
     1681 tcagcggcta tcacatccgc gaagccggtg cgaccggcgt gcaggagctc gcgttcacga
     1741 tcgccgacgg catcggttac tgccagctcg gcatcgaggg cggactcgac atcgacgagt
     1801 tcggcccgcg cctctcctac ttctgggacg tccacaacga cttcttcgag gagatcgcga
     1861 agttccgcgc cggccgccgc atgtgggcca agatcatgcg cgagcgattc ggcgcgaaga
     1921 atccgaagtc gtggctgctc cgcacgcacg cgcaaaccgc aggcgtgtcg ctgatggcgc
     1981 agcagccgct caacaacgtc gtgcgcacca cggtgcaagc gctcgccgcc gtgctcggtg
     2041 gcacgcagtc gctccacacg aacagctacg acgagacgta tgcgctgccg accgaagatg
     2101 ctgccgagct cgcgctccgc acgcagcagc tgatttacga agagaccggc gtcgcgaacg
     2161 tcgtcgatcc gctcgccggc agctacttca tcgaaacgct caccgatcag gtcgaagcgg
     2221 ccgcgtggga gtacatcaac aagatcgacg cgatgggcgg catcgtgaag gccgtcgaag
     2281 aaggctaccc gcagcgcgag atcgcgcgcg ccgcgttcga gttccagcgc gaggtcgaca
     2341 gtggccgccg cacgatcgtc ggcgtcaaca agtacacgac gccgggcgaa ggcgatcaca
     2401 tcccgacgct caagatcgag ctcgagatcg agcacagtca gatcgagaag gtaaagaatt
     2461 tccgcggtca gcgcgacgag gcggtcgtcc agcgcgcgct cgcggcggtc aaggccgcgc
     2521 tcaaggacga caagcagaac gtgatgccgc cgatcctcga ggccgtgaag gtcaacgcca
     2581 cgctcggcga gatctgcgat ctgttccgca gcgagctcgg cgagtaccgc gacccggcgt
     2641 acctctagaa ttgctgctcg gggaacgcgc tcagcaagta gcggggcgac gccgactcgg
     2701 gatcgatgac gatcgcgccg tcggcgttcg tgacgttgcg atgaactagg tgtgcgctcg
     2761 cgccgccgga gctggtcgcg aacaccgaca cgtgcgcacc gatcacgaac gcgtcgtcga
     2821 gccgcttgat cgcctcgagc tggttcgcct cggtgaagct gctcctgccg acgccgagcg
     2881 tgctgacgta gtcgagctgg acgcccggat gccaaccctc ggcccacgga tcgccggcga
     2941 gcggcgcgtt gatgtcgtcg agccacacgt gcggatcggg cgacgtcgac aacacgttga
     3001 tgaccatgcg gtacgcagcg ccgtccgtcg tcacctgcag cacgacgtgc gtcgagttcg
     3061 gcatcgcaca gcggttgttg ccgggcatca cgacggcgag gatggtgccg tccagccggc
     3121 cgaacgcgtt tgtcagtgcg gtgccaaacg tcgaggcaca cgccgcgctc ttgtcgttgc
     3181 tatcaacgtc aaaaggcgtg tcgggcgtcg agccgtcaat gaggggcgac ggctcgacgg
     3241 tccgacacgc aaacagaaac acaagcagcg cgcgcacgtg ggggagttgg acacagtgcg
     3301 cgcgctagag caaccagtta cgggtgactg ctcgaatcgg ccgggtcctt gtgggccgcg
     3361 acctcgtccc agtccgcgta gccgtcgccg tcgcggtcga tcgcgagacg ctggccctca
     3421 cccttcggta cgcacgtgaa cgtgaccggc gggatgatgc cctgcgcgat acgcgaccgc
     3481 agcgtcgcat ccgacaccgg cggcacgaac accgacgcga gctgccagct gccggccttg
     3541 tacagcgcgc ccaggttcgg gcccgcggtg ctcgtcgact tgccgacgag gtcgcactcg
     3601 cccgcgttcg cacgcgcctc gagcagatcg aggcgtgcgc ccgccgttga accgttcgtc
     3661 ttcgtcaccg tgatctgctg accgacgacc ggagcgaagt tcgaatcgaa cgcgatcatg
     3721 aacgcgacga tcgccttgcg cagcgcgaag ccaccgtcac cgtcgaacac cacctggccc
     3781 tggttgagct tgttgacgtc gacgaacggg atgccgaacg ggttcggcgg cacgacgatt
     3841 ccgcccgcga gcaggatgtt ctcggtggcg cggatgaaca cgaggccggt gaagaagtgc
     3901 tcgagctggc cgagcgagcc gtcgtgctgg aagccgaagc cgcgcacctg gtcgacgacc
     3961 ggaacgttcg ggccctggag cacgatcgtg cccggctgga tcgaatccgg cgagctgccg
     4021 aacatgccga tcttcgtgta catgttgcgg aggtgcggga ccttgaagat ttgggtctcg
     4081 aactcgaacg acagccgacc gtcggtaccg aagaagcccg ggtgtgggct cgagccgctg
     4141 ttaccggcga ggtcaagcgt gtggcagccg ttgcagttgt ggaagcgatc cgagggtagt
     4201 tcctgcgtgc ccgcgtggtt gaagtagaag tcgcggccct gctgctgctc cgccgtcagc
     4261 gagttgtcga ggttgcggac cgggttcggc gggtacatca cctgcaggat gaacgtcgcg
     4321 aactcgccca tgtcgtgttc gctgagctga tcggcgcgac cgacgaggcc cgggaacgcg
     4381 acgttgaacg aggtgaacgc cttgaactcg tcgaagaggc ccgcgttcgg ctgcgccgac
     4441 gtgatcggct ggccgttgcc atcgaggaac ggcgcaccgc tctgctggac ggcgccgtta
     4501 cggtcaccgc gccagtgcat cgcgccatgg ttgtccatgc cgcggaggct ctgcgtcgtc
     4561 atcgggccct tgagcgggtt gttggccgcg aactccggtt cggtgtatgg caggagctgg
     4621 atgatcgccg ccggcgggag cgcgaacacg ttgccgagcg tcgggatcgg cagcgggccg
     4681 ccaccaggat taccgaggtc ccacgcgagg tcatccttgt cgccgccgat gtggcaactc
     4741 gcgcaggcgg ccacgccgtg ccccgacgtc aggtttgcgt cgtagaggta cttgcggccg
     4801 tcgaccacgc tctcgggctc gggattgaac atcgggacgg tcccgatctc cttgcgcatt
     4861 gcgaggtcga cgatcttgat gctgttgtcg aagcgcgtga gcacgtacgc gcgaccgcgg
     4921 gcctcgtcga gcgcgacgcc cgtcgggccg ccgccgctca gcgtgacctg gctggtcagg
     4981 gtcgggctgg cgacacccga ttcgatatcc gcggtgcgat agatcgcgag cttcgacgag
     5041 ccctgcgcga cagtgtagat cgtcgagccg tttgccgaca ccgcgatgtc ctgggcgaag
     5101 gcaacgctga gtgccctctc cgacgccgag ccgtcggtcg tgtaatccac gtgcgggttg
     5161 aggtcacgcg gtgataccgc gccgctcgtc ggatcgatga ccgtgatccg ggtgtcggca
     5221 atatgaccaa ccacgctgga gaagcccggc gtgtggcctt cgaagcggac gtcgttgtgc
     5281 gcctcggtgt tcgtgacgta gaccttgccg ctctgcgggt tgaccgccat gttgaacagc
     5341 gtcgtaccga cgtgcgcgta cgtccgcgtc gcgaccggcg gattcgcggt cgcatcgatc
     5401 gcgaatacgt cgttgtcggg cagcttgatc ttgacgaacg cgtcgaacac cgtgccgtac
     5461 gcgtcgaccc agtggccgtt cacgaacttc acgatcagac cggtgcgcgg ctgcggatag
     5521 gtcacaccat tgacggtgac cgtctgcgga cccggcatgc cgttcggata tacggtggcg
     5581 accgcgtact cggagaccga ggtcgtctgg ttgcccgaga ggaacgcggc ggcatagacc
     5641 gtgcggccgt cgccagacac cgcgagcgcg cgcggcgtat cggcaaacag cgtcagcttc
     5701 gtgagccggt tgccgcctgg cgcgctgccg agattgttcg catcgaacac ccagacgtcg
     5761 gcgcgaccga cgcccggggt ctgcagatcg tacgcgtcgc cggtgttctg gccgcggtgc
     5821 gcggtcgtga tgaacgcgcg atcgcgattg gtacccgcga acacgatgtc acgcggctcg
     5881 tcgccgacga gcagggtccg cgtaacggtt gcgacgccgc tgctgctgac gctgacgacg
     5941 ctgaccgagt cggacacgtg gttcacgacc cacacttcgc tatcgttgcg tgcggcgacg
     6001 gcgacaggct cgaggccaac cgcgatcgag cccttcgaga cgagaccgcc gctaccgacg
     6061 ctgtagatct cgagtcggtt gtcgggcgtg ttcgccgcga acagcatctt tccattcggc
     6121 gacaacgcga cggggcgtgt ctggagactc tcgaacaacg tatgcgggtc cgagctcgct
     6181 ggcagtttgt taccgccgag ctccgatggg tcctccgttg actcgtcggt gcatgcgggc
     6241 atcgccaaaa acagacaaag caggcccaac ttcctcatca tgctttcagc gttacgggaa
     6301 attactttga actcaaagca gtatgtttgg tggcgtcgct cgcagttgct acgcgatgtg
     6361 accgcaacag aaagctgcgt aaggacccag gcagaaaggt ctgtggcgtc gctcgcattc
     6421 gctacggcgc gtgaccgcaa aatgccccaa aacggcgaga ttccggctca gccgctagca
     6481 ccagttagtg ccacgcaact acgtgttagc gttccgaatc gcacttcaga gcacacgtgc
     6541 gagctgcagc aatgcgactt ctgtcgccgg cacgcggcgc agctcgccgt cgatcagaat
     6601 caggtctgca accgtgtcgg cgcgcggcgc gatgcccatg tcgtaaacga catcgttgcc
     6661 gcgcacgcgc agcgtgcgaa tgccgccgtg cttgcgcgtc ttcgccgatt cggtgaccca
     6721 cgggccgagc tcgtgcacgc acttcgcgtg gccgctgtga cctgcgatct gcgtgatgcc
     6781 gacgggcagc gtgcgcggat cgaagcggcg cggccgcgcg gccgacagtg cccacgcctt
     6841 atcggcgtca ggacgctccg ggttcgaagg ccggtgatac aggagccccc cgccctcctc
     6901 gccgttgccg ccgctcacgt gcaacggcgc gagcgacagc ggcgtattcg cgccgctctg
     6961 ccagtcgccg cgcaccgcat cgactgccgc gtgcaactgg gcctcgagtg ccgctgcgat
     7021 ctcggccggc ggtgcgccgc ccttgccgag catgccgagc tcgcgcgacg tgacgccagc
     7081 gtgcgtgatc agcgccgtgc gcccgtcggg aagctcgccg acgcacgcga ggtgaaagcg
     7141 ctcggcgagc aagagctcct gcacgagcgc gcgctgttcg gtcgtgaacg atgcgtagtc
     7201 gcggccgagc acgcccggcg gcggtagctc ggggaacgcg ggaacgtact cgtcgcgtgt
     7261 gcgttgctcg gcagccgctt cgcctgcggt gtgctcggtg tcgtcgatcg atcgcgcgag
     7321 caagcgcgcg gcctcgaatc gctcgtcggc gaggtgcgcg agctccatca cgcgcgacgc
     7381 gtcgtgatta ccgaacagca gcgttacctg atgactcggg tggctcgcga gccagcgcaa
     7441 cagccggagc ccatcctggg ccgcgccgac cggatcccgg aagtcgtaat cgaagtgatc
     7501 gccgatcgag atgagaacca cgtccggcgc gatgcggtcg ccgtccagca cgccgtggca
     7561 cgcgaggacc tcgagcacct ttgcgaacgg tgcttggggg tctcccatta cgaacgtcct
     7621 cggcatagcg gccggcacta tatcctcgtc gcgctcagtc cgtcgccgag gcggcgctca
     7681 cgccggcagt tctgcgatgc agcgtatttg tgatgaccat cgtcgtcagg ccgagcagcg
     7741 cgagcacgac cgtgcgcagg cgattgagca gggtgaacaa caggcccgcg acgggtgcgg
     7801 caccgaggag gccatacagc acgtaattcg tcccatccgc gataccgagt ccgagaggga
     7861 tgatgttcga aatccacgtg atcagaatgc caacactcag cgacgcgacc acgagcggcg
     7921 cggtcatcgg aatgtccgcc gcatacagca cgacaatcgt cccgacccag ttcagcacgc
     7981 gggagccaag tacgccggcg agcccgcggc cgatgcccga gtcgcgcgcc gtcatgatgc
     8041 ttcgcagccg cgcatcgatc gtcgcgacct tcgcgcgcca gcgctcagcg cgtgccggcg
     8101 agatgatcga gaacgcagcg aggccatcga tcaacgtgcc gaccacgccc cgtcgcacga
     8161 acaacgcgac gatgatcgca aacgcgacga gcaccgcgag gccgacccac acgagcaatg
     8221 ccacctgccg cggcaaatcg agcaacagtg ccgtcagcgg tacgccgagc acgatcgccg
     8281 caatgccgac gtacatcgtc gtcaggttga acatcacgac cgcggacacg gcgaggtcca
     8341 ccggcacggt gcgcacgagc atcgtcacct tcaccggctc gccgagcgaa ttgcccggtg
     8401 tcagtcggtt gatcgccatg ccgctgagct gcgccgcgta gacgcgccaa tacgacacgc
     8461 gctgctttgg ccgcaagaag ccgtaaatcg cgaacgcatc gcacagcgcc gagacgaggt
     8521 cgatcgcggc gatcaccgcg aaccatacgc cggtcgacac gatcacgttg cgaatcgcgg
     8581 tccagccgag atgagcgacc acgaagccga acgccaccgc gccgatcgcg aagatcacga
     8641 ggttgaacat cgtgctcgcg cggaaccggc ccacgcccga gcttaccagc cacgccaatg
     8701 gccgtcgtcc gtacgcccgg tccgggccgt ggccggacga accggacagc gacgcgcaaa
     8761 cgcgcagagt gattgcacgc gggcttggcc tgcgccgtga agcatgcagc acgcatgaaa
     8821 cgacgtcctc ctcatccgct cgaacatcat ccttccttcc gcgccgtgat cgcggcagcg
     8881 ccaccggtcg ccgagctcgc acgcgagctg tccgacgacg ggcgcgagct ggtcggtgca
     8941 ctcgcgatcg tgtgcggcga tctcgcgcaa ggattcgcgt ctcctccggg gagccgtgcc
     9001 cgcaacgagg cccaccaccg cgcctggacc acggtccgcg agatcgatcg tgcggtcacc
     9061 gccgcgcgga ttcaccggcg cgcaccggca cgtctcgttg ccaaggcgca gcgcgccatc
     9121 gaccgcgccg acgtgatgat cgcagcgctt ccgggcgtac ttcctggttg aaaacacagc
     9181 gttccagatg gcgatgcgac atttaggatc aaccctcctc tagggggcat aggatcccaa
     9241 cgagaggaca gacgttcatg cgcatccatc acgcagctgt gttgttgttc gcggccgtat
     9301 tggccaccgg ttgtaagaaa cgaggcacgg gcagtggcgg tggcggcggc ggcggcggtg
     9361 gctggttgat cggtagctct ggtctgatgg tcaacatcaa cgacgatgcg ctcgtcggcg
     9421 agtacgacct cggctccgaa gcgaacctga acgcgattgc gtgccggtat caagcggaag
     9481 cctgggtcgt cggcgacgac gcgaccgtcc tttatacgaa cgacggcggc acgtcgtggg
     9541 tctcgcagac cgtgccgacg acggcgcatc tgcgcacgct cgcaacgcaa gacgatggtc
     9601 ccgtgttcct tggcggcgac ggtacgttcc tcgtgacgag cgataacggg ttgacgtgga
     9661 cgcagctcgg cgatggcgtt acgtcgttcc gctcgatgtc ggccgcgcag gccggcaccg
     9721 gcgtgctcgc gctcgcggac gacggcgggc tgtggtcgta tgacggtggc acgctcacgc
     9781 atcgcagcac gatcgctggc gcgcgcgccg tgcatcagac gcccgacggt tcgatcgtga
     9841 tgaccgcagg ctcgggcatc gcgcggtcgc tcgacggtgg cgtgacgtgg cgcccgctca
     9901 cggtcgatcc gtcgatcgtg ttcgacgact tgcgcgtcaa cgaagatggc actgccgtcg
     9961 ccgtcggcgc caatggtgcg atcgcgaacg tcgcgctcaa cggtacggtc tcgctgcaat
    10021 acgtcggcga ctcgacgctg cacacgatcc acatccacga cgacgagatg ggttacgccg
    10081 gcggcgacaa cggccaggtc ttgatcacgg aggactcggg tctcacgtgg cggattggcc
    10141 cgaacgtcgg acgcacggtg cgcggcgtcg acggcatagg cttcaaccat cgctaggcga
    10201 tcgctctcgg cttagtcgcg gcgcacgcgg ccggccgaca cgctgtcgcg acggcgcacg
    10261 ccactaggcg agccaccgac ggtggcgcgg ggactgcaac ccagcgcctt ctgtgatcgt
    10321 tcgagatcga agtctgtacg gaatgatcat ggtggttgcg gcgggctctg cacacgcggc
    10381 gcacgcgcag ccggtggaac cggaaggcgg tgcaagcgcc gacgccgcgc cagcaccgac
    10441 gacgtcgcaa ccatcgccac ttgcgatcga tcagctcacc ctgagcgacg atctcatcgc
    10501 ggcggttggc ctgcggccga tcggagatcg acgcgtcgaa gcgcgggttt cgaccggcaa
    10561 cgacgcgacg caccaggcgg cactgcgcgc caccgacacg attctcggcg ccggcctcga
    10621 gctcaccggc agcgcattgc gcagcgacgt cctgggtgac caaagcgagg ccgccgcgcg
    10681 catcgcgcat acgagcgatc accacgtcgc cggactgcac gcgacgtaca ccgccgacca
    10741 cgtagggttt ggccgccaat cgttcctcac cgacatgcgg accgctacgt atggcgcggc
    10801 gtggacggag tcacgagcgc cgggccgcgt cacgctcgag ctgtacggcg aacagcagac
    10861 gctgcgtgat acccggcagg tcggcacgct cgagctcgac ggcagcttgc tcggcgcaca
    10921 cgtcggcttc acctcgcggt ccgtccaggc atttggcctc gatcacgagt tctccggcga
    10981 catcgacgtc atgcaggcga acggtgtggc gagcgagacc gacggtgatc ccgacgagat
    11041 gcacatgacg atgttgccgc gcacgcggca tggccggcat cggttcttga gcgcgtacat
    11101 ccacgacacc gtgcgggtaa tcgaatcact cgacgtgcac ggcggcttcg tgttcgagca
    11161 ctggcgctgg ctcacgaaca tcgcgtcgat ctacgggccc gatctgggcg agtcgatgga
    11221 tcaagacgct gccgtgatca gcagcttgat gatcggaccg aaggtcggcg cgctctatcg
    11281 cgtgacgccg aacgtggcgg tcaccgcaaa tgcgtatcgc acgctccgca ctccgacatg
    11341 gcagcagctg atgcggccgg tgcagaacgg cgaggtcgtg acggttgccg acgatcagct
    11401 tcaccccgag acagtgaccg gcgcccagct cggaccgacg gttgtcaccc cgaagctcga
    11461 ggcgcgagtc gtggggttct ggaacgaggt cgcggcaccg atcgccgaca tcgacgtcgg
    11521 tcccgcagca cgcgagacga cgaacctcgg ccacgcccgc gagatcggcg tcgacgcgag
    11581 tgcgagctgg cgcatcgcga agtcggtgct cgccggggcc ggctacacgt atacgaacgc
    11641 gcgcatcacc gacgctgcga gccatccggc gcttgcgggc aacgtgcgcg cgcagacgcc
    11701 gcgccaccgc gcgactgccg cactcaccta cgaccggcca acgatcctca cggtgaccgg
    11761 cacggtgcgg ttcgcggatc agcggttcgc caacgccggt aacacgatcg ccctcgcacc
    11821 ggtcgccgtc gtcgacgcga tggcagcgcg caagctgacg cacggcctcg ccggcttcgt
    11881 cagcgtcgaa aacatcttcg accgccgctt cgtctggaac caggctggcg tcgatacgat
    11941 cggcgcgccg cgcctggtcc aagtcggcgt gcgcctcgac agcgcacgct ggtaaccggc
    12001 tccgccaatg gtactcacgt agtgcttcgt gcacgtgctc gtgctcgtgc acgttctgcc
    12061 aaccaccccg acgttctcca acatcgtccg caagaagggc cgcctatcgc ggaagccgtc
    12121 gcaacgacaa gcgaccccgc tcggcacagc cgagacgcgg ctggtcttgc gaggcgctcg
    12181 acggcggtac cccgatcgga caaccgagac gcgcctgctt tgccaggcgc tcgagtgcga
    12241 gcacgtcgcg cgggtaatcg tgcacgtgca cgtgcacgat gcacgtggca cgtgtacgtt
    12301 gatccaccag gatcgcgcct actgcttgat cggcatgccg tagagatcgt gcatctcggc
    12361 ttcgccgagg tcgagcttgt cgacgtccgg cttgatcttc gacaggtcgc caacgatcga
    12421 gatgaccatg ctcttggacg ggatcaccgt cttcgcgacg gtcttcacgt ccttcgccgt
    12481 caccttgcgc acgttcgcgg catagctcgc gtaccagttg tccggcagcc cgaagatcgc
    12541 gatgtcggcg aacgcgcccg cggtggacgc gttcgtgtcg aacatcgacg gcagcgcgcg
    12601 gatcaagttc tgcttcgatt tctcgagctc ctccgccggc acgtcgaccg tcgcgaggtt
    12661 gtcgagcatc ttgaagatct cggcgaggcc cgcgccagtc tgcggcgtga cgatcgcggt
    12721 cgagatcacg aatggacccg gcgacaggcg gtagtcctcg cccgagcgcg cgccatacgt
    12781 gatgcccatc tgctcgcgca gccgctgcac gagacggctc gtgaagccac cgccgagcgt
    12841 gctcgacagc acctcgaact ggaagtagcg cttgtccttg cgatcgatac cgacgaggcc
    12901 gatgcgcacg tccgactgcg ccgcgtcctt gcgatcgacg agcaagaggc gcttcgcgat
    12961 cttctcgggc ttcacggcca ccttcgccgg cttcttcaca ccggccggct tccacgcgcc
    13021 gagcgtcgcg tcgagctttg tcttgagcgc cttcgcgtcg aagtcaccga cgatgatcag
    13081 cgtcatcacg gccgggttcc agtgctcttt gtagaacgtc tggacgtcgg cgaccgacag
    13141 gtccttgaac gtctcgcgca ggcccgtcat cggatggccg tacgcactgc tgagaccgta
    13201 cagtgccgcg ttgagcatca atccggcgac ttcgcgcggg cggtcgcgac gcagctccag
    13261 tgacgtgctg cgatcgccct tcacgcgatc gaactccttc tgttcgaacg ccggctgcgt
    13321 caggatcttc gtgagcagat cgatcgtcgg atcgagcgtc ttcgtcagcg tcgacaccga
    13381 gacctgtgcg aagtcgacat cgacgcctgc gccgatgccc gcaccgagtc ggtcttgctc
    13441 ctcggcgatc gcgatcgcgg acaggccgcc tgcgccttca tcgagaaggt ccgcggtgaa
    13501 cgacgcgagg ccggccttgg agtcgggatc cgcggcagcg ccggcgccgg gcgcgatgag
    13561 caccatcgac acgatcggca gcgcgtggtt ctcgaccagc atcagcgaca tgccgttctt
    13621 cagcttgctg cgcttggcaa ccggcggctt gaacgcgggt tccttggcag ccgcaggcgc
    13681 cttcgtccag tcggtgatcc ccgacttgtc gaacggcgct gcattgggag ctgcagtcgc
    13741 cgtcgcagcc gcgagcaata cacaaagaga aatcgtggcg cgcatcactt caccaccttc
    13801 ttacccgggc gaatcgtcag ctggacgcgc gccgttggct tgagatattt cgcagacacg
    13861 cccttgatct gatccggcgt gaccgcgcga tagcgcgcga ggtccttcgc gaagtagtcc
    13921 gggtcgttcg tgaacacgtc gtagtttgcg agcaggatcg cgcgctgcag cgtcggctcg
    13981 aggccgttca agaagcccgc ctcgtgcgaa ttctgcgcgc gctcgagctc cttcgcctcg
    14041 ggtgcggtcg tcgacagctt cgcgacctct tcgctgatct ccttgacgag gcgcttcgga
    14101 tcgacgcccg gcttcgcggt cgcgacgatg cggaactcgc cgccgagcgt ctcgccctcg
    14161 aagtatgcgc ggacgtcttg cgcgatcttc tcgtcgtaca cgagccgctt gtagagccgg
    14221 ctcgacttac cgtcggcgag gatcgcagac gccatcacga gcgccggctc atcttgcgtg
    14281 tagtgcgccg ggccgcgcca ggacaagtac acgcgcggca cctgcacgtc gtcggtcgta
    14341 tcgacaacga tctccttcgt gatcggcggt ggcgtcttgt actgcgggcg ctgcggatcg
    14401 gtgcccttgg ggatccacga gaagtacttc tcgacgagct tcttgacgtt gtcgaacttc
    14461 acgtcgcccg cgatcaccat cgtcgcgttg ttcggcaggt agtacttctt gaagaaatcg
    14521 gcgacgtcag gaaccgacgc ggctttgagg tccgccggat aaccgatcgt cgaccagtga
    14581 tagccgtgct ccttcggcca caggttctcg tggaggatga tctccgccat gccgtacggc
    14641 tggttctcgt agctctggcg gcgttcgttg agtacgacgt cgcgctggtt gtcgagcttc
    14701 ggcttgtcga acgtgtcgag caggcccgcc aggcgatcgg catcgatcca cagcatctgc
    14761 tcgaggaagt tcgacgccgc gacgttctga tacacggtca tgtcgctcga cgtgaacgcg
    14821 ttcgaccagc caccggcggc ctcgagcagc ttgtccatca ggccgtcagg tagatgcgcc
    14881 gagcctttga acatcaggtg ctcgaacaga tgcgcgaagc ccgtacggtt cgcgacctcg
    14941 tccttgctgc ctacgcggaa ccacgtcaca ccgtacgcgg tgggagcctt gtgatcctcg
    15001 atgacataca cacgcatgcc gttggcgagg tcgaatttct gatacgtgat tttgggttcg
    15061 tccgcgtacg cgggcaccgc cagcgcgacg aggaacagga gcgtgagccg tctcatggcg
    15121 cggacgctaa cacacgcgcg gcgccgcact atgtgcttag gtgcctcgtt cccaaacggc
    15181 cgtcgacgac gggggagcct gattccgggc acgtggccgc ggcgcacaga ctgcatgccg
    15241 cgatctgcta tgcgctccgg tatctccgat tcgaccgtcg agcgttttca cgcgttcctc
    15301 ggcgaggaag accaaggacg ggcgcgagcg atcggcgccc tcgacgcgtt ctttgctgca
    15361 gcgtcagctg cgctgctgct cctcgaccgt gacctccatt gtgtgcaggc gaacgagcgg
    15421 ctctcccggc tcactcggct accgctcgaa gaacaccgcg accggccggt ccgcgagctc
    15481 gtgccggtag cgctggagag caaactgcgc gagatcctgc agtcggggga accactcgac
    15541 aaagagccga tcgagctcga tggacgctcg ctgctcggca cgttctttcc ggtattcgac
    15601 gccaaccaca acatcagtgg gctcggcggc atcctgatcg actacaccga gcacaagcgg
    15661 ctcgaagccg agctccgctc cgcgatcgag attcgtgagc gtgtgctcgc ggtcgtgtcg
    15721 catgatctac gcaacccact cgggaccatc gagcttgcgg tgtcgacgct gcccgagtca
    15781 gcccgtgacg atcgcgaagc cgcacgccgc atcgagatca tcgagcgtgc gacaaagctc
    15841 atggagacac tgatcggcga cttgctcgac atggcgacga tccagactgg cacgctcgct
    15901 cttcatttcg ctgacgagaa tgccgatgcg gtcgtgcgcg aagccatcga gctgcaccat
    15961 ccgctcgcgg aagaagcaca cctgaggctg gtcgacgaga cgcaccttga gggtgttcgc
    16021 gtacgctgcg acaaagcgcg cgtgctgcag gtgctcggca atctgatcgg caacgcgatc
    16081 aagttctgca aacgcggaga tgcgatcacg gtacgcggct acgccgacgg gcgatcgctc
    16141 gtgctcgagg tcgtcgatac cggtcccggt atccctgcag ccgacattcc gcacctgttc
    16201 gagcagtact ggtccacagc acgcggccgg caacgcggca ccggtctcgg gctcttcatc
    16261 tcgcacgcga tcgtcgcagc gcacggcggc gagctcaccg tcgcgagcac tctcggcgtc
    16321 ggtaccagct ttcgcattgt cctacctctc ggctgagctg cccggccgca acgtgcgcgc
    16381 gcgctcgaaa tgctcgagcc aactgtgcat catccgtcac tcacgtcgac gcatggcgat
    16441 tgcacagtta ccgacgacaa tggaaaggcc gtcccagacg ctcgtgcgac tcgagctgga
    16501 gacccacgcc taccatcgcg acgccgacca gccctggctc gacctgctcg acccgttcct
    16561 gacgcgcctc gcgtacgccg agcagctcgg ccgcatgtac tgcttcgaat gccccgtcga
    16621 atccgcactc gcgtacacgc cccacgtgac gtcactgatc ggccaccgcg cccggtctcg
    16681 gctgctcgcg cacgacctgt tcgcgctcga ctactcgcaa gcccgcctct cgccacgcct
    16741 gatcgcgccg tttccgacgg tcgccagcgc gctgggctgg ctctacgtcc tcgaacgtag
    16801 cgcccggctc tatgacctcg tggtgcgcaa cgtcgccgcg cgcctacccg gcgcaccgac
    16861 cgaatacgtc gcagaccccg cagcgcctgc gcgatggacc gcgctcggcg aggtcctcga
    16921 ccgcgtcgcg cgaaccgcgc ccatcgccga tcagatcgtc ctcgcggcac acgacgcgtt
    16981 tcgcagcttg ctggactggt atgtcggtga acaggcattg cgtcgcggcg cctgaccaac
    17041 taggttcccc gcctgctgtt ctcagatgca catcctagcc tccgccgcgc gattgctgcg
    17101 aaaggctatt ccgaaccaac cccggtgcaa gccgctgtac tcgcagcggc acctgatcgc
    17161 gacctcttgg tctctgcgca aactggttcc ggcaagacgg tcgcgttcgg gcttgccctc
    17221 gcgccgcgct tgctcggcga tgcggagcgg ttcgaaaaag ccggcgcacc gcgtgcgctg
    17281 atcgtcacgc cgacacgcga gctcgcgcaa caggtgcacc gcgagctgca gtggctctac
    17341 ggtgagactg gcgcgcggct caccacgtgc gtcggcggca tggatccgcg acgcgagacg
    17401 catgcgctcc agcatggcgt ccacatcgtc gtcggaacgc ccggccggct ctgcgatcac
    17461 ctcgaacgtg gcgcactcgt gctcgcgcgc atcgacgcgg tcgtgctcga cgaagccgac
    17521 gagatgctcg atatgggttt ccgcgaggac ctcgagcgct tgctcgatgc tgcgccgacc
    17581 gagcgccgta cgctcatgtt ctcggcaacg attccgaaac caatcgcgac gctcgcgaag
    17641 cgctaccaga agggcgcgct gcgcatcgcc gccaccgccg aaggcgatcg ccaccgcgac
    17701 atcgagtacc acgcgatgcc gatcatcccg cgcgagcgcg atctcgcagt cgtcaacctg
    17761 ctccgctacc acgacgcgcc cggcgcactc gtgttctgcg cgacgcgcga cggcgtcacc
    17821 cacctcgcgg cgaatctcag cgagcgcggc ttctcggtgg cagcgctgtc gggcgagctg
    17881 agccagcgcg agcgcaacca agcgctgcag gcgctgcgcg atggccgtgc ccgcgtgtgc
    17941 gtcgcgaccg atgtcgccgc ccgcggcctc gaccttcccg acctcggcct cgtgatccac
    18001 gccgatctgc cacagaacgc gcaggtgctc gtccatcgca gcggccgcac cggacgcgcc
    18061 ggcaagcacg gcctcgcgat cctcatcgtg cccgacaatg cgcgtcgcta cgtcgagcgc
    18121 atgctcgcca ccgcgcacct cgacgcgaag tggcgcctgc cgccgtccgc cgacgatatt
    18181 cgggtccgcg accaagatca gctcgtgctc gagctccgcg cactcgccga cgaaccgagc
    18241 gaggatgatc gccgcgtcgc gcagacactg ctgaccgagc gcagcgcaga ggccctcgtt
    18301 gccgcgctcg tgcggtcgcg tcgcgacgca cgcccggcgc ccgaggagct gacgatgccg
    18361 ccgtcgatgc gcccgaagcg gccggagccg cagcgcccgc agcgggtcga ccgtcgcaag
    18421 gagcaggtcc cattcgaggg ccccgagcgc cgcggcgcga agccacagcg tccacagccc
    18481 gaacggccgc gcggaaacgg cgagcggcgc ggcgacgctg tgtggttcac gatcaatgtc
    18541 gggcgcagca agaacgccga tccgaagtgg ctcatcccgc tgctctgccg gcgcggcaac
    18601 gtcaccaagc gctcgatcgg caagatccag atcctcgcac gcgaaacccg ggtcgaaatc
    18661 gccgccgata ccgccgagca attcgccgag gcggtgcgcc gtcccgatcc caaggatccc
    18721 cggattcaca tcgagcgcgt cgacgcagag atctaagccg cggtcggcag caggatcgtg
    18781 accgtggtac cgacgccagg ctcgctctcg attcgcatgc ggccgccggc gcgaccgatg
    18841 aggcgctgac agttcgcgat gccgaggccg gtaccctccg gacgtgtcga gaaccacggc
    18901 gttccgatct tgcgcagcac ctcgggtgac atgccgatgc catcgtcacg cacctgcagc
    18961 tcgagcatgt caccttgcgt gcgcgcctcg agcgcgacat ggcgattcgg ctcgccgcgc
    19021 gcatgtaccg cttgggtcgc gttgttgacg agattgacga gcacctgcgt gaggtcgaca
    19081 gcagagatgc ggacgtgcgg cagtgacttc gagccgcgat agccgatctg cgccgtgcga
    19141 ttctcgacgg tgatccgctg gaacatgttg acggtgaact ggatcaccgg cgtcggatcg
    19201 atcaccggcg tctcttgcgg gcccttgtta cggatgtagt ccgagagccc gccgatcatg
    19261 tccttgatca gtgtcgaggc ttgcttcgcg tcctgcacga tctctggcgt gtcatcgact
    19321 ctctggagca accgggcctt cagacggtcg tcgatatccg acgcctcgat tgccgcatgc
    19381 agcgtcggta cggcatctgc caccgcgtgc agctcgtcga gcaggttgtt gaccaccatg
    19441 atcggcgtct tcaggtcgtg aacgtagagg gccgtcaagc cgccgagcga tgcgagtcgt
    19501 tcggtctcga gaagccggcg tagcagctgt gccgagtcct tgccgaagtt ccacagctcg
    19561 gtcgcccatc gcaggacttg gaccagctct tcgcggtccc acggcttgac gatgtaacga
    19621 gcgacaagtc cctcgttgat cgcgcgcaag atcggatcga cgtccgagta cgcggtgatc
    19681 accatgcgga tcgtgttcgg atggcgttcc ttggccctcc gcaacagctc gaggccatcg
    19741 acctcgggca tccggatgtc gctgaccaga acggccacgt cttcgcgatc gagagcctcg
    19801 agcgcggact ttgcgtcctc gaccgtcttg atcgtgaatt ccgacttcag tgactgctcg
    19861 aagacgatgc ggttcgcgcg ttcgtcatcg acatacagca cgagcggtgg tttgggcatg
    19921 tccgccatgg accgcgccaa aatcataccg cgtcggccga actagccgca tcgattcggc
    19981 ctttagttcg agcgcgaaaa cgctgcgccg gccggttcag gtcagttcac gacacgcaga
    20041 cggggccagg cgctcggctc ggccggtgcc ggcggcggcg ccttctgctt cgtggccatg
    20101 accgcgaccc ggtcgacctt cggcatcatg atatccggat cgatccggac gtcgatgaca
    20161 accgggcccg gcgccgaaag caagcgcacg tcgaactgat gcttgccgtc gacgcgcatc
    20221 gtgaccgcgc cgaggccgcg cgcgatcgag cagatgtcga gcggaccggt cgagtaccgg
    20281 gggcgccggc cgtagacgtt ctggtggccg ttctcgacca tcccgagccg ttcgtcgttg
    20341 aacacgaaca cgcggatcgg caattgctcc tgcacggccg tcgcgatctc gaacgcgttc
    20401 atcgcgaaac agccgtcacc gcagatcgcc gcgacactgc ggccggggtg agcgagctgc
    20461 gcgccgatcg ccgcgccgat cgattggccc atcgaaccga ggccggtcat cacgacaaac
    20521 gcgtcgggat cgcgggtctc gaggtactgg accgcgtaga ggaagtgctc gccggaatca
    20581 acggtataga tcgtgtcgcg cggcaacatg cgctgcagct cgacgatcgc gtcctgcggt
    20641 gcgatgcgat cgagccgcgt cgacgacgag atgtgatagc gctcgacgcc attcgttctc
    20701 ggacgctcga tcaccttggg ctcttcgcgg cgatcggcga ggccaccgag cagctcggac
    20761 gcggacgcga cgatcgcgtg agtcggcgcg tagctcttgc cgagctggcg cgcgtcgatg
    20821 tcgacgtgga tcagcgcgcg ggatgcttgg agcgcaggcg cgaagccgtc ggtggatacg
    20881 tcgccgaggc tggtaccaat cgcgagcacg acgtcgacat tgttgtcgag gtagcgacgc
    20941 gaggagcggt ggccgcccag gccgagcaca ccgagcgaga gcggatgcgt ttcagggaac
    21001 acgcccttgc ctttcggcgt ggtggcgacc gggcacgagt agcgctcggc gaccgcacgc
    21061 aggcgggttg gcgcgccgcc accgcggacg ccgctaccag cgaggatgag cgggcgctcg
    21121 gcgtggatca gaagctcggc gacctcgtcg atcgtttcgg cggcgaccag gttcgtcatc
    21181 gtgaccgagc cgcccgaccg tggcgtggtc acgttcgcgg tcatgatgtc gatcgggagc
    21241 gtgagcacga ccggaccttt gcggccggac agcgctgtcg cgattgcgcg gcggaggaca
    21301 tggggcagcg cggagggtga cgcgatctcg gcagccatct tggagatgtg gcgcgacatc
    21361 tcgacgatct ggaggccatg ggacgacgag tcctgaagca cgccgcggcc ctgggcgccg
    21421 cgcggtacgt cgccgacgag cagcagcacg ggcaggctgt cgcaccatgc cgatgcgaga
    21481 ccggtcatcg cgttgagcgc gccgggaccc gaggtcaccg cgaccacgcc gagcttgccg
    21541 gtcgtgtgcg catagcccgc ggcagcgaac atcgcgcccg cctcgtgctt ggtcgtgatg
    21601 acgcggatgt cgttgtcgag aagcgcgtcg tggaccggcg agatagcgcc gccgggcagg
    21661 ccaaacacga cgtcgacgcc ggccgaggcc aacatggaaa cgagcaggtc ggcaccgcgc
    21721 gtggacggcg cgaccggttc aggcggtgcc acagacggtt gcggcggacg ggccagctcc
    21781 ttgagatcgt acggcttggc gactgcgcgc tcggcgccgg ccgccttggc aacgagctcg
    21841 agctcgggcg tcatcgcgac gctggcaagg atcacgtcga tgtcgcgcgt cgcgggattc
    21901 gccttgatac gccggcaggt ctcgacaccg tcgagcccgg gcatgtacac gtccatgatc
    21961 acgagatccg gcagtgcatt cccgatgatc aggaggccgt cgatcgcgtt gctcgcgacg
    22021 agtagctcga tcgagcggtc gtcacgcagg gcacgctgga gcgctcgaag ctggatgtca
    22081 tcgtcgtcga cgacgagcac gcggagcggg gcgccgacag tacgaagacg ctttcgggac
    22141 tcccacatcg gcacgcctta taccgtgacg gcggattttc gaaacgagat tgttagagag
    22201 ccccgatggc aggttggcgc gctcctcgct ttcgtgtagc aaccagggcg ctagtaaccg
    22261 caaggagtat cgttggttgt ggctgcggtc gtcaccgtac aggaccttca gaaacgattc
    22321 ggaaacagac gcgtgctcat gggcgtctcg tttgcggttc acgagcgcga ccgcattggc
    22381 ctgatcggcg caaacggtgc aggcaaatcg acactgctga agatgatggt ccacggcgcg
    22441 agtggcacct ccgaccccac gctcgagccc gatgacggaa tgatcacatg gcgacgtgac
    22501 ctcgtcctcg agtacgtcgc tcaggagccg cagctagcgc ttgatgaaac ggtcgcgacc
    22561 gcgctcacgc gcgaggaggt gcccgaccac gaaatgcata cagtggcagc ggcactgcaa
    22621 ttacccccgc taacctcgcg cgtcggccag ttatcgatcg gcgagcgccg gcgtgtcgca
    22681 ttggcccatg cgctgctcgg caagcccgac gtgctcgcgc tcgatgaacc gaccaaccat
    22741 ctcgatgcgc gcacgatcga atggctggag acgcggctcg gcgcgtggcc gggcgcactc
    22801 atcgtcgtga cacacgatcg ttactttctc gatcgcgtgg cgacccgcat tgtcgagatc
    22861 gatcgcggca aggcatacgc gtacgaaggc gactacgaag acttcttgct cgcgcaggcc
    22921 gaccgactcg cgatcgaggc ggaggccgaa tatcaacgcg cgtcattcgt gcgccgcgag
    22981 atcgattgga tccggcgtag cccggaggca cggcggacga aggcaaaggc gcgcatcgat
    23041 cggtttgacg ctgcggtcgc ggccgcaccg gcactcgagg acaagcgccc cgcgacggcg
    23101 cgactcacgc ttccgtcggg accgcggctg ggcagctcga tcgtcgagct gaagaacgta
    23161 tcgcgcacgc tcggcggtaa ggtcctgttc aaagatctct cgctcgtgat gaagcctggc
    23221 gatcggatcg gcatcgtcgg tccgaacggc gccgggaaga cgacgctcat caagacgatc
    23281 ctcgggctcg agccgccgga tagcggcgcg gtcgtgctcg gcgtcaacac gaagccggcg
    23341 tacttcgagc aaggtcgcag cgagctccgc gacgagctca cggtcctcga agaggttggc
    23401 gacggttacg actgggtcga gctgcccgac ggcaagatcc acgtgcggag cttcctccgc
    23461 atgctcgcgt tccccgagaa catcgccgac acgaagatcg ggcagctctc gggcggcgag
    23521 cgcaatcgcg tccagctcgc gcgcttgctg cggcgcggcg gcaacttgct cgtgctcgac
    23581 gagccgacga acgatctcga tctgccgacg ctcggcgcgc tcgaagaagg gctcttgtcg
    23641 ttcggaggct gcgcgttgat cgtgtcgcac gatcgctggt ttctcgatcg cgtcgcgact
    23701 gcgatcctcg cgttcgaggg caacggcaac gtcacgttgt gggaaggcag ctacacgttc
    23761 tacgcggagc gccgagccgc gagcgcgaag ccgcagcgac aggacaagcc cgacaaggac
    23821 aagccgaagc caacgcgcgc cgcgccgccg cgcaagctca cgttcaagga gaggcacgag
    23881 ctcgccggga tggaagctgc gatcaccgca gcggagacga aggtcaccga gctcgaggcg
    23941 acgctgtcgg atccgagcgt gttcaaggat cgaccgaccg aggtgcaaac actcgtcggc
    24001 gacctcgacg cggcgcgagc ggaggtcgag cgcctgttcg cgcgctggca ggagctcgaa
    24061 gcgattccga aggaatgagc taggagctag cgaatgagct cgtcgatgac cgcgaacagt
    24121 gcaacgcggt cgaacggctt ctcgatcgtc gggttcgcga cctgctcgaa gaacgacttc
    24181 gcctgactcg tgaacgcgcc gccggtcatg aagaccatcc tgtccacctg ctccggcgca
    24241 gtggtctgga gtcgcgcgtg tagctccatg ccgctgatct ccggcatcat cagatcgcag
    24301 agaatgacgt cgaagcgttc gccgccttcg attctgtcga ggacctcgcg agcgctggcc
    24361 accgcggtga cgtcgtggtc ggcgagcatg cgacgaatcg ctcgcgcgag ctctggctgg
    24421 tcgtcgacga tcaggatctt tcgttgacgc accacctcag tcacacgaag tgccccctgc
    24481 acgtgtgatc aaactctatc atcacgagcg ggtgctgggg tcaccgctgg accggttcgc
    24541 acgtccgcgc attccgcaca ttagattgtc ggctagcgca cgaacggacc ttcggttgct
    24601 tctcgatcca cgcgatcgat atcgtagaag tgcttgccac tgagccggtc gatgtgcgcc
    24661 tcgatcgcag cgaggtggcg gcgctcgtca gtcagattct cgacgacgat cgcttgacca
    24721 atcggcggca tgtagatctc cgacgcgcga tcgtaggtcc ggttcgtcaa cttctcgttc
    24781 atgcgcattg cgcgcaaggc gccgagggtg ccgcggctgc gtaggcgcgt catgccttcg
    24841 aggatgcccc ccttgagatc gcggtgcgcc tcgatcggcg tcccgcccag gtcgcgaacg
    24901 atttgtgaca actccatcac gtgccgctcg tggtcaccga ggaacgcgtg gaggtcagac
    24961 cgcgcagccg gatcgtcgat gtggaggatc gcctcgcggt aggtcccggc cgcatcgaaa
    25021 tcgagctgga tgagcttgtt cagctggtcg cacacctttg cgatccaatg ctcgtgcttg
    25081 gtcatgttgt gctgcatgac ttcttctcct tcgcagacga agggctgcat cgcaggtgcc
    25141 cgctacgcta cgagaacagc tgaagaaaac tcatgcggct gtctcgtgcg accggtgcga
    25201 cgggtgcgac ccctgaatgc ccgtgaacgt cgcgcgaaga cccgcgctat gttccgccgg
    25261 tatgcagcga cgtaccttct tgttgtccgc ggcttcgtcc gcggttctgc tctcgttcaa
    25321 aagcaagctc tcgttcgcca agggaggcga aaagatgccc tctgaaccgc tactcgcacc
    25381 ctggactggc ccgtacggcg gtgttccgcc gttcaacaag atccaggtca aggacttcaa
    25441 gcccgcgctc aagaagggca tggacctctc gcgcgctgat atcaaggcga tcgcggagag
    25501 caaggacgcg gcgacgttcg agaacacgat cgcggcgctc gaggactcgg ggcgcgcgtt
    25561 ttcgcgctgc ggctcgatgt tcggcacgtt cacgtcgacg atgaactcga agccgatgca
    25621 gacggtcgag aaggagatgg cgccggtgat cgccgcgttc tccgacgaga tcacgcagaa
    25681 cgaagcgctg ttcgcgcgca tcaagacggt ctacgacggc cgcgccaagg ccaagctcac
    25741 gcccgagcag aaccggctcg tcgaggtcac gtaccaaaac tttgcgcgca agggcgcatc
    25801 gctgggcgcg aaggagaagg cgcggctcaa ggagatcaac cagaagctcg cgacgttgtt
    25861 cacctcgttc tcccagaacg tgctcgccga cgaagaaggc caggagatcg tgctcgacaa
    25921 ggaagccgac ctcgccgggc tgccggagtc gctgcgcgcg tcgatgaaga ccgccgccga
    25981 gggcaagaag ctgaagggca agtggctcat cagcaacacg cgctcgtcgg ccgagccgtt
    26041 cctcacgtac tcgtcgcgcc gcgatctacg cgagaaggtg ttccgtatgt ggtcgagccg
    26101 cggcgagtcg gccgatcaca acaacctgcc cgtgatgacc gagatcctcg cgctgcgcgg
    26161 cgagcgcgcg aagctgctcg gcttcaagac gcacgcgcac tggatcatcg acgacaacat
    26221 ggccaagacg ccggacgccg cgatggcgtt gatgatgaag gtgtggaagg cggcgaccgc
    26281 gcgcgtgaag gaagaagtcg ccgacatgca gaaggtcgcc gacgccgaga aggccggcca
    26341 caagatcgag gcgtgggact accgctacta cgcggagaag gtgcgcaagg caaagtacga
    26401 cctcgatcag gacgaggtga aggcctacct ccagctcgac aagctgcgcg acgcgatgtt
    26461 ctgggccgcg ggccagctct acggcctcga gttcaagccg atcaaaggcg tgcccgtcta
    26521 tcacaaggac gtcacgacgt atgaagttgt gcgcggcggc aagcacgtcg gcctttggta
    26581 cttcgatccg tacgcgcgcg ccggcaaacg ctcgggcgcg tggatgagcg agtaccgcac
    26641 gcaggaaggc ttcaagaccg cggtcgcgcc gatcgtctcg aacaactcga atttcgtgaa
    26701 gggcaaggcc ggcacgccgg tgctcatctc gtgggacgac gcgacgacga tgttccacga
    26761 gttcggccac gcgctgcatg gcctcaactc ggaagtgaac tatccgacgc tcgccggcac
    26821 gaacacgaag cgcgacttcg tcgagttccc gagccagctc aacgagcact ggctcacgac
    26881 gccggaggtc ctcaacaagt tctgcgtcca ctaccagacc ggcaagccga tgccgccgga
    26941 gctggtcgcc aagatcgaaa aggcgaagaa cttcaaccag ggcttcaaga cggtcgagta
    27001 cctggccgcg gcgatctacg acctccgcat ccacatgacg ccgaccgaca agccgatcga
    27061 tccggcgaag ttcgagcaga cgacgatgaa ggagatcggc tgcccgaccg agatcgtgat
    27121 gcgtcaccgc ccgcccgcgt tcctccacat cttctcggac gacgcctact cggccggcta
    27181 ctacgtgtac atctgggccg acacgctcac cgcggacgcc gcggaagcgt tcgtggaagc
    27241 gaagagcttc tacgacaagg cgaccgcgaa gaagctgcac gacaacatca tgagcgtcgg
    27301 caactcgatt ccgcccgacg tcgcgttccg ccagttccgc ggccgcgacg tcgataccga
    27361 cgcgctgatg cgcgatc
//
LOCUS       DASUFX010000701         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2057006, whole genome shotgun sequence.
ACCESSION   DASUFX010000701 DASUFX010000000
VERSION     DASUFX010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057006"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFV99_28340"
     CDS             complement(<1..592)
                     /locus_tag="VFV99_28340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063332.1"
                     /translation="MTRASALDAAARSDTAVGSSDSSRRAPTEDRRISDSNQAWLGKI
                     VDGRYRVLEVIGRGGMGVVYRVEHLRMGKVAAMKVLHRDLAGDPDVIGRFEREAAAIS
                     KLFHPHTVQVFDFGTAGEALYLIMEFVRGIDLAQMIQRDGPMPWSRAAPLLAQVCGAL
                     QEAHELGIVHRDLKPENVLITRTAGGRDYAKVLDFGL"
     gene            complement(723..1772)
                     /locus_tag="VFV99_28345"
     CDS             complement(723..1772)
                     /locus_tag="VFV99_28345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063333.1"
                     /translation="MEKGRRPSRSVTGFGLRTSDFGGAPLVLARSLHILVAGALVACG
                     SSDPAPSAPRIGNVLAAVLAAADHTRTPWRCAALDTPALADAPLTTGDRHWQLGGHML
                     RRTDNDDTVAIAVIADAADASPVTIAALARLRAELEKASPDLVLTLGGMGSTQAELEA
                     TLGTLGDHAPWPVVALPGDLEPMSAHVAALTNLRKRAINVLDGRLVRWIEQPGITIGT
                     LPGAGARERLVASDDGCQWRAEDVAKLYTELTEKPGIRVAATSEAPRTTSDGEPAGEL
                     GLVPPQPIELALSAPVAPAASPPRTGSRDGARIVLSPGTADGVRRLPDPHTPNAGLLV
                     VRAGTWTWRPLAAAK"
     gene            complement(1739..2632)
                     /locus_tag="VFV99_28350"
     CDS             complement(1739..2632)
                     /locus_tag="VFV99_28350"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5063334.1"
                     /translation="MRALLLLVCVALSATAFAQGNSVAYRAKQDDTLDVIAAEYYGDR
                     AQAKLIVAENKLKQKRVQPGQRLKIPVTREIVTDKGDTFESLAQTHLGDARRAVVLAD
                     FNERDVSDTPAIGTTISIPIQITHTAQGTETLASIATAYWNDPKQAEVLRRYNFLDKP
                     SIEKGESVIVPMIKVRVRKPAPLDTDAKERRDEQHKVATEAERVLPLARTAWLAGEFK
                     VIRETLEPLVAKADYLETPEAIEVGLLLGKAQLAFKDDKHATESFTHVLNRKPSHVLS
                     AYAESPKVIDAWKKAGGQVAP"
     gene            complement(2629..3975)
                     /locus_tag="VFV99_28355"
     CDS             complement(2629..3975)
                     /locus_tag="VFV99_28355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063335.1"
                     /translation="MLERIGNCRIVEEVASGGMAVVYRAVQDPLGRTVAIKALKTSAA
                     AEENVVTRFEREAKSLANLQHENIIHVYDFHRERGAMFIVMEYVQGIDLYDLLEKCGR
                     LPYDVAAIIAMQVARALDYVHYRGIVHRDIKPANIMIARTGGVKVMDFGIARDQSFGD
                     LTEAGTGIGTPAYMSPEQVLGDKLDARSDIFSLGVVLYQMVTGKKPFVEDERKSAMHK
                     IRLEKHESARSVNPEVPRELNNIIDRCLEKQPRDRWRSAQHMVMALERFLSRHVEMNH
                     HARLVLFMRAQNVITELEAEEYLNPAALGAGAGALQQPNLQARNVVRAGFVAHGIVAG
                     VLLLFLGLIHVAPVSATPQSEALLAKERKRGYLRIDAHPWARVFVDDKEVGVTPIGEP
                     FELREGSHKIRFEHDWYEPVERTITIVADTKDNAQSLTCDFEATKAKLKPGKTVPQ"
     gene            4417..>4834
                     /locus_tag="VFV99_28360"
     rRNA            4417..>4834
                     /locus_tag="VFV99_28360"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          810 a         1527 c         1601 g          896 t
ORIGIN      
        1 cgagcccgaa gtcgagcacc ttcgcgtagt cgcgaccgcc ggccgtgcga gtgatcagca
       61 cgttctccgg tttcagatcg cgatgcacga tgccgagctc gtgcgcctcc tgcagcgcgc
      121 cacacacctg tgcgagcagc ggcgctgcgc gcgaccacgg cattggcccg tcgcgctgga
      181 tcatctgcgc gagatcgata ccgcgcacga actccatgat caggtacagc gcctcaccgg
      241 cggtaccgaa gtcgaacacc tgcacggtgt gcggatggaa cagcttcgag atcgcggcgg
      301 cttcgcgctc gaagcggccg atcacgtcgg gatcgccggc gagatcgcga tgcaacacct
      361 tcatcgcggc gaccttgccc atgcgcagat gctcgacccg atacacgacg cccatgccgc
      421 cgcggccgat cacttcgagc acgcgataac ggccgtcgac gatcttgccg agccacgcct
      481 gattcgaatc ggagattcga cgatcctcgg tcggcgcacg ccgcgacgag tcgctcgaac
      541 cgaccgcggt atccgaacgc gcggccgcat cgagtgcact ggcgcgcgtc atgtccgcgc
      601 cgcactcgcg gcaatagtgg ctcggctccg caaacgaagt gcgacagcgc gggcacacga
      661 atttttgcgc cggtgtcgag gacacgcagg accatgttac cgctcgccct ggcgggtgcc
      721 cattacttcg cggcagcgag aggccgccac gtccacgtgc ctgcccggac gacgaggagg
      781 cctgcgtttg gcgtgtgcgg gtcaggcaac ctacgcacgc catccgctgt ccccggagac
      841 agcacaattc gcgcaccgtc gcggcttccc gttcgtgggg gcgacgcagc gggggcaacg
      901 ggtgcgctca gcgccagctc gatcggctgc ggcggcacga ggccgagctc gcccgctggc
      961 tcgccgtcgc tggtcgtccg cggcgcttcg ctggtcgccg ccacgcgaat gcccggtttt
     1021 tcggtgagct cggtgtagag cttcgccacg tcctcggcgc gccactggca accgtcgtcg
     1081 ctcgcgacca gtcgctcacg tgcgcccgca ccgggcaacg taccgatcgt tatgcccggt
     1141 tgttcgatcc agcgcaccaa ccggccgtcg agcacgttga tcgcgcgctt gcggagattt
     1201 gtgagcgccg cgacgtgcgc gctcatcggc tcgaggtcgc cgggcaacgc gacgaccggc
     1261 cacggcgcgt ggtcgccgag cgtaccgagc gtggcttcga gctcggcctg cgtcgacccc
     1321 atgccgccga gcgtgagtac gagatcgggg gatgcctttt cgagctccgc gcgcaggcga
     1381 gcaagcgcgg cgatcgtgac cggcgaggcg tcggcggcgt cggcgatcac ggcgatcgcg
     1441 acggtgtcgt cgttgtcggt gcgacgcagc atgtggccgc cgagctgcca gtgccggtcg
     1501 ccggtggtca gcggcgcgtc ggcaagtgcc ggggtgtcga gcgcagcgca ccgccatggt
     1561 gtacgcgtgt gatcggcggc cgcgagcacg gctgcgagca cgtttccgat ccgcggcgcg
     1621 ctcggtgccg gatcggagct accgcacgcg accagcgcgc cggcgacgag gatgtgcagc
     1681 gaacgcgcga gaaccagcgg tgcgcccccg aagtccgaag tccgaagtcc gaagcccgtt
     1741 acggagcgac ttggccgccg gcctttttcc acgcgtcgat cactttcggc gattcggcgt
     1801 acgcgctcag cacgtgcgac ggtttgcgat tgagcacgtg cgtgaacgac tcggtggcgt
     1861 gcttgtcgtc tttgaacgcg agctgcgcct tgccgagcag caggccgacc tcgatcgcct
     1921 cgggcgtctc gagatagtca gccttcgcga cgagtggctc cagcgtttct cggatcacct
     1981 tgaactcacc ggcgagccac gcggtgcgcg cgagcggaag cacgcgctcc gcctcggtcg
     2041 cgaccttgtg ttgttcgtcg cggcgttcct tcgcgtcggt atcgaggggc gccggcttgc
     2101 gcacgcgcac cttgatcatc ggcacgatca ccgattcgcc cttctcgatg ctcggcttgt
     2161 cgaggaagtt gtagcggcgc agcacctcgg cttgcttcgg gtcgttccag tacgctgtcg
     2221 cgatcgacgc gagcgtctcg gtgccttgcg cggtgtgcgt gatctggatc gggatcgaga
     2281 tcgtcgtgcc gatcgcgggg gtgtcgctga cgtcgcgctc gttgaaatcg gcgagtacga
     2341 cggcgcggcg cgcatcgccg aggtgggttt gcgcgagcga ctcgaacgtg tcgcctttgt
     2401 cggtgacgat ctcgcgcgtc accggaatct tcaggcgctg gccgggctgc acgcgcttct
     2461 gcttcagctt gttctcggca acgatcagct tcgcctgtgc gcgatcgccg tagtactcgg
     2521 cggcgattac atcgagcgtg tcgtcctgct tcgcgcgata cgcgacgctg ttgccctgcg
     2581 cgaacgcggt ggcgctcagc gcaacgcaga cgagcagcag cagcgctctc attgcggcac
     2641 cgtcttgccg ggtttgagct tcgcctttgt cgcttcgaag tcgcaggtga gcgactgcgc
     2701 gttgtccttc gtatcggcga cgatcgtgat cgtgcgctcg acgggctcgt accaatcgtg
     2761 ctcgaaccga atcttgtgcg agccctcgcg gagctcgaac ggttcgccga tcggcgtgac
     2821 accgacttct ttgtcgtcga cgaacacgcg tgcccacgga tgcgcgtcga tgcgcagata
     2881 cccgcgcttg cgctccttcg cgagcagcgc ttcggactgc ggtgtcgcgc tcaccggcgc
     2941 gacgtgaatc agcccgagga agagcagcag cacgccggcg acgatgccgt gcgcgacgaa
     3001 gccggcacgc accacgttgc gcgcctgcag attcggctgc tgcagcgcgc ctgcgccagc
     3061 gccgagtgcg gccgggttca aatactcctc ggcctcgagc tcggtgatca cgttctgcgc
     3121 gcgcatgaac agcacgagcc gcgcgtggtg attcatctcg acgtgacgcg agaggaagcg
     3181 ctcgagcgcc atcaccatgt gctgcgcgct gcgccagcga tcgcgcggct gcttctcgag
     3241 gcagcgatcg atgatgttgt tgagctcgcg cggaacctcg ggattcacgg agcgtgcgct
     3301 ctcgtgtttc tcgaggcgga tcttgtgcat cgcgctcttg cgttcgtcct cgacgaacgg
     3361 cttcttgccg gtgaccatct ggtacagcac gacgccgagc gagaagatgt cgctgcgcgc
     3421 gtcgagcttg tcgccgagaa cttgctcggg gctcatgtac gcaggcgtgc caatgccggt
     3481 gcctgcttcg gtgagatcgc cgaagctctg atcgcgtgcg atgccgaagt ccatcacctt
     3541 cacgccaccg gtgcgcgcga tcatgatgtt cgcgggcttg atgtcgcggt gcacgatgcc
     3601 gcggtagtgc acgtagtcga gcgcgcgtgc gacctgcatc gcgatgatcg ccgcgacgtc
     3661 atatggaagg cgaccgcact tctcgaggag gtcgtacagg tcgatgccct gcacgtactc
     3721 catgacgatg aacatcgcgc cgcgctcgcg atggaagtcg tagacgtgga tgatgttttc
     3781 gtgctggagg ttcgcgagac tcttcgcttc gcgctcgaag cgggtgacta cgttttcctc
     3841 cgcagcggcg ctggtcttca gcgccttgat cgccacggtg cgtccaaggg gatcctggac
     3901 agcgcggtaa accactgcca ttccaccgga agccacctct tcgacgatgc ggcagttgcc
     3961 aatgcgttcg agcatggtaa ctctggccct gaggttacga cggtttggcg ttgtaaccac
     4021 cccggtttcc ccccaattgt cgtgacctcg cgtcagacaa aacgggaatc gaggttggag
     4081 agggatcggg cggaaaaccg agatcaattt gatctccaaa ttcgccgcga tgtcgaatgt
     4141 ttaggtggtc ggtgacaggg ttggcggaaa aatattcgat cgatgttgac gactcaaaac
     4201 tgatcccgta taagcagcat ccctcgacgg caccacgcct tcgagaccga cgagaaggca
     4261 cagctttccg tcgggatcgg tctctgaaaa ctggatagca agtctgttac ggaagattgt
     4321 gagtttctgt ctgagataga aaccattcca cgtcggacga agccttcccc ccggatgtgc
     4381 ttctatcagc cgacgtagag cgggattcaa aacttttcta tggagagttt gatcctggct
     4441 cagagcgaac gttagcggcg ggcctaacac atgcaagtcg agcgagaaaa gggcttcggc
     4501 cctgagtaaa gcggcggacg ggtgagtaac acgtaggtaa tcttcccttg agcgggggac
     4561 aacgagccga aaggctcgct aataccgcat acgtttccag aggccccggt ctttggaagg
     4621 aaagctaggc cgcaaggtcc ggcactcgag gaggagcctg cgtcgcatca gctagttggt
     4681 ggggtaacgg cccaccaagg cgaagacgcg tagctggtct gagaggatga tcagccacac
     4741 tgggactgag acacggccca gactcctacg ggaggcagca gtggggaata ttggacaatg
     4801 ggcgcaagcc tgatccagcc acgccgcgtg agtg
//
LOCUS       DASUFX010000702         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_945966,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000702 DASUFX010000000
VERSION     DASUFX010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945966"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFV99_28365"
     CDS             <1..432
                     /locus_tag="VFV99_28365"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5063336.1"
                     /translation="DGNTTATDGCSATCNIEPGFSCTGTPSVCTPNCGDGVVAGSETC
                     DDGNTAAGDGCSASCALEPGWSCQMTTCDEVCGDGMIVGAEACDDGGTVDGDGCSATC
                     TVEMPPGSPDSGCCSAGGNPTGSFALTLFTLGIVLRRRRRA"
     gene            474..1010
                     /locus_tag="VFV99_28370"
     CDS             474..1010
                     /locus_tag="VFV99_28370"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX5063337.1"
                     /translation="MRPIYTIGHSARTYDELVAALTAWHVTTLVDIRHFTRSRANPQF
                     NAASLRRRLPRAGIAYVTLPALGGRRGRAKDVDPDRNAGWTHAAFKNYADYAETSAFA
                     KALASLLARAKNETCAIMCAEAVWWRCHRRIVADYLITRRVPVFHIFTPTKAVRAART
                     PFAKLDRRTRTLRYPATK"
     gene            1341..1832
                     /locus_tag="VFV99_28375"
     CDS             1341..1832
                     /locus_tag="VFV99_28375"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5063338.1"
                     /translation="MENETVSLPDHSQVLVFPPAIPATGFLLGVVLNWLVPLGSVVDG
                     PLRTTLRVAGGAVFLLGIAGFAWMIVTMKRARTPIHNARTPTTLVHSGPFRFTRNPMY
                     LFGSIWYAGLALALVELWSLALLPLTVITLHYAVVLREEAYLLRRFGDAYRDYQARVP
                     RYW"
     gene            complement(1882..>2738)
                     /locus_tag="VFV99_28380"
     CDS             complement(1882..>2738)
                     /locus_tag="VFV99_28380"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5063339.1"
                     /translation="QLEPRIRQLSRELLAGRTEIELVGELATPLPTLVIAEMIGIPAA
                     DRERFVRWADAINGLSYAAVGGLAAQEMIAAYYRVRDEAEPYVAALVDERRREPREDL
                     ITRLALAEIGGERLTAAELFGFFQLLLAGGTETTTNLIANAILCAAQYGVPRDLPLFV
                     EEVMRYRSPAQIMFRQTKQAVELHGVTIPRDAFVLVALGAANRDPAAFPDPNRFDPAR
                     DPNPHVGFGHGIHHCIGAALARLEAHVVLEELFALGTPVLASSEPWTPRRALHVLGPA
                     ALPVVFRG"
BASE COUNT          445 a          913 c          902 g          478 t
ORIGIN      
        1 gacggcaaca cgacggccac cgatggctgc agcgcaacct gcaacatcga gcctggattc
       61 agctgcacgg gtacgccgag tgtctgtacg ccgaactgcg gagacggcgt cgtcgcgggc
      121 agcgagacgt gcgatgacgg caacaccgcc gccggcgacg gttgcagtgc cagctgcgcg
      181 ctcgagccag gctggagttg ccagatgacc acgtgcgacg aggtctgcgg cgacggcatg
      241 atcgttggag ccgaggcgtg cgacgacggc ggaaccgtcg atggtgatgg ctgcagtgcg
      301 acctgcacgg tcgagatgcc tccgggctcc cccgattcag gttgctgctc ggccggcggc
      361 aatccaacag gttcgtttgc actgacgctg ttcacgttgg gcatcgtatt gcgtcgccgt
      421 cggcgcgcgt gatagcggtc aactgatgcg tcgctcgctg agagcgtctt gcgatgcggc
      481 cgatctacac gattgggcac tccgcccgga cctacgatga gctcgtcgcc gcgttaaccg
      541 cgtggcacgt caccacgctg gtcgatatcc ggcatttcac gcgctcgcgc gcgaacccgc
      601 agttcaatgc tgcatcactg cgacgcaggc tacctcgcgc tgggatcgcg tacgtcacgt
      661 tgcccgcact cggtggacga cgcgggcgcg cgaaggatgt cgatcccgat cgcaacgcag
      721 ggtggacgca cgcggcgttc aagaactacg cggactacgc cgagacttcg gcgttcgcga
      781 aggcccttgc ctctctgctc gctcgcgcca agaacgagac ctgcgcgatc atgtgcgccg
      841 aggcggtgtg gtggcgatgc caccggcgca tcgtcgcgga ctatctgatc acgcgacgcg
      901 tccctgtgtt ccatatcttc acgccgacga aggccgtgcg cgctgcgcgg actccgttcg
      961 cgaagctcga tcggcgaacg cgcacgcttc gctatcccgc gacgaagtag ggcatttccc
     1021 aagttcgccg ctggttgccc cgaaaacgaa acaagcggca ttgctgccgc tcgatcggtt
     1081 tctcggggtt cgtgcgcgag gggggactcg aacccccaca cggttaagta ctagcactga
     1141 actagggcac ttcggaaggc gctctcggtg gatgacgcga gccgcacgat ctgggctatc
     1201 gcgagtccag aggtgcgcca gatgctccat acgttcgcga ggtggccgcc ggatcgctac
     1261 cgggcatggc ttctcgacac gctcgtaagc gcgctgctcg aaccaatgtc gacacaacgc
     1321 aaaagacgaa ggagatgagc atggagaacg aaaccgtttc gcttcccgat cactcacagg
     1381 tgctcgtctt tccgcctgcg atccccgcca ccggcttctt gctcggcgtc gttttgaact
     1441 ggctcgttcc gcttggttcg gtggtcgatg gtccgctgcg aaccacgctg cgcgttgccg
     1501 gtggcgcagt gttcctactc ggcatcgccg ggttcgcgtg gatgatcgtg acgatgaagc
     1561 gagcccgcac gccaattcac aacgcgcgta ctccgacgac gcttgtccat tctggtccct
     1621 ttcgctttac acggaacccg atgtacctgt tcggttccat ctggtatgcg ggcctcgcgc
     1681 tcgcgctcgt cgaactttgg tcgctcgcac tgctgccgct caccgtgatc acgttgcact
     1741 acgccgtggt gctgcgggaa gaggcgtacc tcttacgtcg cttcggcgac gcctatcgcg
     1801 actaccaggc tcgcgttcct cggtactggt gaacccgggc agccagcctc ctcggcagcc
     1861 agcctcctcg atgtctcgaa ctcagcctct gaagacgacc ggcagcgcgg ccggtccgag
     1921 cacatggagc gcgcggcgcg gcgtccatgg ctcgctgctc gcgagcaccg gcgtgccgag
     1981 cgcgaacagc tcctcgagca cgacgtgcgc ctcgagccgc gcgagcgccg cgccgatgca
     2041 gtggtggatg ccgtggccga agccaacgtg cggattcgga tcgcgcgcgg ggtcgaagcg
     2101 gttcgggtcg ggaaacgccg ccggatcgcg gttcgcggct ccgagggcaa cgagcacgaa
     2161 cgcatcgcgc gggatcgtga cgccgtgcag ctcgaccgcc tgcttcgtct gtcggaacat
     2221 gatctgcgcc ggcgagcggt agcgcatgac ctcctcgacg aacaacggca ggtcgcgcgg
     2281 gacgccgtac tgcgcagcac acaggatcgc gttcgcgatc agatttgtcg tcgtctcggt
     2341 tccgcccgcg agcaacaact ggaagaagcc gaacagctcc gcggccgtga gccgctcgcc
     2401 gccgatctcc gcgagcgcga ggcgcgtgat tagatcctca cgcggctcgc gcctccgctc
     2461 gtcgacgagt gccgcgacgt acggctcggc ctcgtcgcgc acgcggtagt acgccgcgat
     2521 catctcctgc gcggccagcc cgccgacggc ggcgtagctg agcccgttga tggcatcggc
     2581 ccagcgcacg aagcgctcgc gatcggcggc cgggatgccg atcatctctg cgatcacgag
     2641 ggtcggcagc ggcgtcgcga gctcgccgac gagctcgatc tcggtgcgcc cggcgagcag
     2701 ctcgcgcgag agctgccgga tccgcggctc gagctgcg
//
LOCUS       DASUFX010000703         7559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1780010, whole genome shotgun sequence.
ACCESSION   DASUFX010000703 DASUFX010000000
VERSION     DASUFX010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780010"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFV99_28385"
     CDS             <1..203
                     /locus_tag="VFV99_28385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063340.1"
                     /translation="FDNNRTSTVTWGQGNGEMCTFLAWTDSEYTWTGGVLTSNTQPTI
                     TDHGDYVEYAYPCSPVGKLVEN"
     gene            complement(200..1336)
                     /locus_tag="VFV99_28390"
     CDS             complement(200..1336)
                     /locus_tag="VFV99_28390"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEX5063341.1"
                     /translation="MRQSIFLGFVLAAAACGGGDSEEQILNPVRDSYNTWIKVEPPGV
                     VCGNNTPYKFFVNYSDKSDNLLVVLEPGGACWDYDSCTGANGIRGAANVNGLPDDHYK
                     LAPFISPFLNRDRPEVSTGDWNFVYIPYCTGDVHTGNNVATYADEAGTASVEFHHDGH
                     HATEEITTWLDENFTHVPKLFVTGCSAGGVGSVVNYHFLRNGIKAAEKGYMLDDSGPV
                     FPSTGYSGPMHMKIREAWNVDSIGTDLPAGFTFEDMGTINTALADEFPNDRLATTYFR
                     RDMDFSLYSYERFYNFPPKDEILRMWDADTQLFTKLYDTRKNLHYFIPYWRNVNDSHC
                     TTVLSFAGSDIEAQNLTLEGWVKDLLADKPIKSAIEDPVPGEDP"
     gene            complement(1434..3161)
                     /locus_tag="VFV99_28395"
     CDS             complement(1434..3161)
                     /locus_tag="VFV99_28395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063342.1"
                     /translation="MGDAPAVEREDDDDDAGTAAPTAHALPAHAHARRAAALSTPPPT
                     ASAGSTPGPQVSQQIAMATLREEELDTARHMIRVGRGLGLAAAMTLPFVGGSQLLRIA
                     LAATILLAVIIGLLVEHRLDRPGQSVEKVLLKLAVSVGPAVYMSALYFGVLSAVQLFP
                     ALILYFFARRESFKSALALFLANAIVQAAMAIALIAGIVEDPGLVSAQLPPIVVAIGH
                     ILLQVGFLAAFLLGRGSYKASREAIAKMQNAMMLAAQREALLHEARQDLDRALAIDAA
                     GRFTDHTFGGYRLGHVIGRGGMGEVYEAFHIDSGENAAVKLLAHRELGNPASRERFVR
                     EVATVQKLSSPHVVRVLTASDQRDEIPYLVMERLRGEDLAHHLRSGKMPPSTLLEMLH
                     QVGSAVEEAWAKGIVHRDLKPQNLYLADATGRPTWKVLDFGVAALDEHSGTLTQGKVV
                     GTPAYMAPEQARGERVDHRADVYALCAIAYRWLTGRPVVGGKDLHNALYQTVHVMPQQ
                     PSTLAELHPDVDAVLAIGLCKEPSERWQTVGELRVALEVAIAGQLDPRARRRAADVLG
                     KHPWGAVRG"
     gene            complement(3212..4096)
                     /gene="sucD"
                     /locus_tag="VFV99_28400"
     CDS             complement(3212..4096)
                     /gene="sucD"
                     /locus_tag="VFV99_28400"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5063343.1"
                     /translation="MSVLVGHNTRLVVQGMSGSAGAFHAKQMVEYGTNVVAGVTPGKG
                     GAKVEGLEKVPMFDTVAESVAQRGANTSVIYVPPPFAADSILEAAAAGIELIIAITEG
                     IPARDMVKVKYVLDRDYPGVVLVGPNCPGVITPGQCKVGIMPGYIHKPGGVGVVSRSG
                     TLTYEVVGQLTKLGLGQSTAIGIGGDPVKGTDFIQALSWFAEDPGTSSIFMIGEIGGD
                     SEERAAQFIKENVKKPVAAFIGGQTAPPGKRMGHAGAIISGGKGAAADKIAALKAANI
                     AVAATPAEMGTTLAKVLG"
     gene            complement(4096..5277)
                     /gene="sucC"
                     /locus_tag="VFV99_28405"
     CDS             complement(4096..5277)
                     /gene="sucC"
                     /locus_tag="VFV99_28405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5063344.1"
                     /translation="MKIHEYQGKQLFQKYGVPVPIGIPAMSAEEAIAAIPKVQQQAGT
                     DVVVVKAQIHAGGRGKGGGVKVAKTRADAEAAVKQIFGMQLKTPQTGPEGKKVQRLLV
                     EQGLAIEHEYYLGMLLDRGTGRVTVMASTEGGMDIEEVAAKHPEKILKQAIDPATGWQ
                     DWQSRTLAKQLGMTGKLSNELGKFLKALTRCYEEMDCSLLEINPLISTKDGKVLALDA
                     KINFDDNALYRHPELEEWRDLNEEDPQEIEAKKWDLSYISLDGNIGCLVNGAGLAMST
                     MDIIKFYGGQPANFLDVGGGATAEKVTAAFKIITSDPEVKAIFVNIFGGIMKCDTIAA
                     GVIAAVKETGLKMPLIVRLEGTNVELGKKMLAESGLNITSAADMADGAQKAVAAAGGK
                     K"
     gene            complement(5296..>7559)
                     /locus_tag="VFV99_28410"
     CDS             complement(5296..>7559)
                     /locus_tag="VFV99_28410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063345.1"
                     /translation="PSEVRAVLTRGLDPAASARWSSMEDLLDALERAGTQPARRKRLI
                     VTLVASALLVAIGVGVFMMRAGDRSKTGCDDPDRQFVEAWSPAVRSALARKTTPANVE
                     RIGEQLDRYRDRWVESYAKACRQRPAKIARQRIACLEGIRDQAAAITTMLGDVDAKVL
                     NVFEPRDALATVASCETASPLAPPGVPRDQPRRDKILHLIGRGFALRGVPAPQLGAAI
                     DALLAEAKPLDWPPLAPLVLVPAGSQYVRLGETTLARATYQRALASLPRDPELRDVRL
                     EGSAYLGLLDTSLAELENPHAAAPPGMKDPKTREAPLHGELTVRITKATNAAGGDPLL
                     LGARSLLAALAYAHAAQWNRYPTGYDEALRLITEARKQFDEIGDVQRSAFAAATEAEV
                     YLMRGDDRALDDALFAARRGLDALEAAKLPRLPQLDDVRAKVAFARRDYKELYRIQQA
                     NNATEPKLASAQLIKGRVVGENAGAAIVVAWQGSLTGHAKRLVMNAHEMTGDLVRVES
                     DGTFMIRANPDWMIMAETKGARSAPQLVGDKPPALKLEPTVTVSGQVESLNLFDVQVF
                     ARYTTAHDHAWELRAPVDKDGSFDLGGLPPGPHVYGTHGPAGTGERTIIAGANPKQIT
                     WSYGQAIEIIVRAKTHFDDGARAWVIRGEHAPESAAQLEALLASAPDVATSKLYPIGA
                     DNTDAGRDTYRTGDRHAVITGNSDGVRYTVCARPTGDSKPTCIPFPFEKTVGIPYDDG
                     RYGAGVSPILLEL"
BASE COUNT         1197 a         2485 c         2498 g         1379 t
ORIGIN      
        1 tcttcgacaa caaccgcacg agcaccgtga cgtgggggca gggcaacggc gagatgtgca
       61 cgttcctcgc gtggaccgac tccgagtaca cgtggaccgg cggcgtgctg acgagcaaca
      121 cgcagccgac gatcaccgac cacggtgatt acgtcgagta cgcgtatccg tgctcgcccg
      181 tcggcaagct cgtcgagaac tagggatctt cgcccggaac cgggtcttcg atcgcgctct
      241 tgatcggctt gtccgcgagc agatccttca cccaaccctc gagcgtcagg ttctgcgctt
      301 cgatgtcgga gccggcgaac gacagcaccg tcgtgcagtg gctgtcgttg acgttgcgcc
      361 agtacggaat gaagtagtgg aggttcttgc gcgtgtcgta gagcttcgtg aacagctgcg
      421 tgtccgcgtc ccacatccgt aggatttcgt ccttcggcgg gaagttgtag aagcgctcgt
      481 acgagtacag cgagaaatcc atgtcgcggc ggaagtacgt cgtcgcgagc cggtcgttcg
      541 ggaactcgtc cgcgagcgcg gtgttgatcg tgcccatatc ttcaaacgtg aagccggccg
      601 ggaggtcggt gccgatcgag tcgacgttcc acgcctcgcg aatcttcatg tgcatcgggc
      661 ccgagtagcc cgtcgacggg aacaccgggc ccgaatcgtc gagcatgtag cccttctcgg
      721 cggctttgat gccattgcgc aggaagtgat agttcacgac cgagccgacg ccgccggccg
      781 agcagccggt gacgaacagc ttcggcacgt gcgtgaagtt ctcgtcgagc cacgtcgtga
      841 tttcttccgt cgcgtggtgg ccgtcgtgat ggaactcgac gcttgctgta ccggcttcgt
      901 cagcgtacgt cgcgacgttg tttccggtgt gcacgtcgcc cgtgcagtac gggatgtaaa
      961 cgaagttcca gtcgccggtc gacacttccg gacgatcgcg attcagaaac ggcgagatga
     1021 acggagcgag cttgtagtga tcgtcgggaa gaccgttgac gttcgccgcg ccgcggatgc
     1081 cgttcgcgcc ggtgcagctg tcgtagtccc agcacgcgcc gccgggctcg agcacgacga
     1141 gcaggttgtc gctcttgtcg ctgtagttga cgaagaactt gtacggcgtg ttgttgccgc
     1201 acacgacgcc cggtggttcg accttgatcc acgtgttgta cgaatcgcgg accgggttga
     1261 ggatttgctc ttcactatca ccgccgccgc atgccgctgc cgcgagcacg aaccccagga
     1321 agatcgactg cctcatggac gctgaaacta cacgagggcc gattggccag accagaacta
     1381 tctcgtagtg aggcgaatcc acaaactcgt acctgcggaa tgcagcacgg gttttagccc
     1441 cggaccgcgc cccacgggtg cttgccgagg acgtcggcgg cgcggcggcg agcacgtgga
     1501 tcgagctggc cggcgatcgc gacttcgagc gcaactcgca gctccccaac ggtttgccag
     1561 cgctcactcg gctccttgca tagaccgatc gcgagcaccg cgtcgacatc aggatggagc
     1621 tcggcgagcg tgctcggctg ctgcggcatc acgtgcacgg tctggtagag cgcgttgtgg
     1681 agatccttgc cgccgacgac tgggcggcca gtgagccagc gatacgcgat cgcgcacagc
     1741 gcgtagacgt cggcgcgatg atcgacgcgc tcgccgcgcg cttgctcggg cgccatgtac
     1801 gccggcgtgc cgacgacctt gccttgcgtc agcgtgcccg agtgctcgtc gagcgcggcg
     1861 acgccgaagt cgagcacctt ccaggtcggt cgtcccgtcg cgtcggcgag gtacaggttc
     1921 tgtggcttga ggtcgcgatg cacgatgccc ttcgcccacg cttcttcgac tgccgagccg
     1981 acctggtgga gcatctcgag cagtgtcgac ggcggcatct tgccgctgcg tagatggtgc
     2041 gcgaggtctt cgccgcgcaa gcgctccatc accaggtacg gaatctcgtc gcgctgatcg
     2101 ctcgcggtga gcacgcgcac gacgtgcggt gacgacagct tctgcacggt cgcgacttcg
     2161 cgcacgaacc gctcgcggct cgccgggttg ccgagctcgc gatgcgcgag cagcttcacc
     2221 gccgcgttct cgccggagtc gatgtgaaac gcctcgtaga cctcgcccat gccgccgcgt
     2281 ccgatcacgt ggccgaggcg ataaccgccg aacgtgtgat cggtgaagcg accggctgcg
     2341 tcgattgcga gtgcgcgatc gagatcttgc cgcgcttcgt gcagcagcgc ttcgcgttgt
     2401 gcagcgagca tcatcgcgtt ctgcatcttc gcgatcgcct cgcgggaagc cttgtacgag
     2461 ccacggccga gcaggaacgc ggcgaggaac ccgacctgga gcagaatgtg cccgatcgcg
     2521 accacgatcg gcggtagctg cgcgctgaca agccctggat cttcgacgat gccggcgatg
     2581 agcgcgatcg ccatcgccgc ttgcacgatc gcgttcgcga ggaacaacgc gagcgccgac
     2641 ttgaatgact cacgccgcgc gaagaagtag agaatgagcg ccgggaacag ctgcaccgca
     2701 gacagcacgc cgaagtacag cgcggacatg tagacggccg gaccgaccga gaccgcgagc
     2761 ttgagcagca ccttctcgac cgactggccc ggccgatcga ggcggtgctc gacgagcagc
     2821 ccgatgatga ccgccagcag gatcgtcgcc gcgagtgcga tacgcagcag ctgcgagccg
     2881 ccgacgaacg gcagcgtcat cgcggccgca agcccgaggc cgcgcccgac ccggatcatg
     2941 tggcgcgccg tatcgagctc ttcttcgcgc agcgtcgcca tcgcgatctg ctgcgagacc
     3001 tgtgggccgg gtgtcgaccc cgcactcgcc gttggtggcg gcgtactcag cgcggcggcg
     3061 cgacgcgcgt gggcatgcgc gggcagagca tgagcggtcg gtgcggcggt acccgcatcg
     3121 tcgtcatcgt cctcccgttc gaccgccggc gcgtcgccca cgccaccata ctagcgcgag
     3181 cgggccgaga gccgaggctt gcgaccggcg ctcagccgag aactttggcg agcgtcgtac
     3241 ccatctcggc cggcgtcgcc gcgacggcga tgtttgccgc cttgagtgcg gcaatcttgt
     3301 cggctgctgc gcccttaccg cccgagatga tggcgccggc gtgacccatg cgcttacccg
     3361 gaggcgcggt ctggccgccg atgaacgcgg ctaccggctt cttcacgttc tctttgatga
     3421 actgggctgc gcgttcttcg gagtcgccgc cgatctcacc gatcatgaag atcgacgacg
     3481 tgcccgggtc ctcggcgaac cacgacaacg cctggatgaa gtcggtgccc ttcacggggt
     3541 caccaccgat gccaatcgcg gtcgactgac cgagaccgag cttcgtcagc tggccgacca
     3601 cctcgtacgt gagcgtgccc gagcgcgaca cgacaccgac gccacccggc ttgtggatgt
     3661 agcccggcat gatgccgacc ttgcactgac ccggcgtgat gacgcccggg cagttcgggc
     3721 cgacgaggac gacgcccggg tagtcacgat cgaggacgta cttcaccttg accatgtcgc
     3781 gcgccgggat gccttccgtg atcgcgatga tcagctcgat gccagcggcg gccgcttcga
     3841 ggatcgagtc ggcggcgaac ggcggcggta cgtagatgac gctcgtgttc gcaccgcgct
     3901 gcgcgaccga ttcggcgaca gtgtcgaaca tcggcacctt ctcgaggcct tcaaccttcg
     3961 cgccgccttt tccgggcgtg acgccggcga cgacgttcgt gccgtattcg accatctgct
     4021 tggcatggaa cgcaccggcc gagccgctca tgccctgaac gaccagacgc gtgttgtgtc
     4081 cgacgagaac gctcattact tcttgcctcc ggccgccgcg acggctttct gcgcgccatc
     4141 agccatgtcg gccgcggacg tgatgttaag cccgctctcc gcgagcatct tcttgccgag
     4201 ctcgacgttc gtgccttcaa ggcgaacgat gagcggcatc ttcaggccag tctccttgac
     4261 cgctgcgatc acgcctgccg cgatcgtgtc gcacttcatg atgccgccga agatgttgac
     4321 gaagatcgcc ttcacctcgg ggtcactggt aatgatcttg aatgcggcgg tgaccttctc
     4381 cgcggtcgcg ccgccgccga cgtcgaggaa gttcgccggc tgaccgccat agaacttgat
     4441 gatgtccatc gtcgacatcg caagcccggc gccgttgacg aggcagccga tgttgccgtc
     4501 gagcgagatg tagctgaggt cccacttctt cgcttcgatc tcctgcggat cctcttcgtt
     4561 gagatcgcgc cactcctcga gctcgggatg gcggtacagc gcgttgtcgt cgaagttgat
     4621 ctttgcgtcg agcgcgagga ccttgccgtc cttcgtcgag atcagcgggt tgatctcgag
     4681 gagcgagcaa tccatctcct cgtagcaacg cgtgagcgcc ttgaggaact taccgagctc
     4741 gttggagagc ttgccggtca tgccgagctg cttcgcgagc gtgcgcgact gccagtcctg
     4801 ccagccggtc gccggatcga tcgcttgctt gaggatcttc tccggatgct tcgcggcgac
     4861 ttcttcgatg tccatgccgc cttcggtcga cgccatcacg gtcacgcgac cggtcccgcg
     4921 atcgagcagc atgccgaggt agtactcgtg ctcgatcgcg agcccttgct cgacgagcag
     4981 ccgctgcacc ttcttgccct cggggccggt ctgcggcgtc ttcagctgca tgccgaagat
     5041 ctgtttcacg gcggcttcgg catcggcgcg cgtcttcgcg accttcacgc caccgccctt
     5101 gccgcgacca ccggcgtgga tctgcgcttt caccacgacg acgtcggtgc cggcttgctg
     5161 ctgcaccttc ggaatcgcag caatcgcttc ttccgcgctc atcgccggaa tgccgatcgg
     5221 aaccggcacg ccgtacttct ggaacagctg cttgccctgg tactcgtgaa tcttcacgag
     5281 cgccggttta tcacatcaaa gctctaacag gatcggactg acgcccgctc catagcgacc
     5341 gtcgtcgtag gggataccga cggtcttctc gaacgggaac ggaatgcacg tcggtttgct
     5401 gtcgcctgtt ggccgcgcac acacggtgta gcggactccg tcggagttcc cggtaattac
     5461 tgcgtggcga tcgccggtgc gataggtgtc gcggcccgcg tcggtgttgt cggcgccgat
     5521 cgggtacagc ttgctggttg caacatcggg cgcgcttgcg aggagcgcct cgagttgcgc
     5581 cgctgattcg ggggcgtgct cgccgcgaat cacccacgcg cgtgcgccgt cgtcgaagtg
     5641 ggtcttcgcc ctgacgataa tctcgatcgc ttggccgtac gaccacgtga tctgcttggg
     5701 gtttgcgccc gcgatgatcg tgcgctcacc ggtgccggct gggccgtgcg tgccatagac
     5761 atgcggaccg ggcggtagac cgccgaggtc gaacgagccg tccttgtcga ccggcgcccg
     5821 cagctcccac gcgtgatcgt gcgcagtcgt gtaccgggcg aatacttgga cgtcaaacaa
     5881 gttcaggctc tcgacctgac ccgacacggt cacggttggc tcgagcttca acgccggcgg
     5941 cttgtccccg accagctgcg gcgccgatcg cgcacctttg gtctcggcca tgatcatcca
     6001 gtcgggattc gcgcggatca tgaacgtacc gtcgctctcg acgcggacca ggtcaccggt
     6061 catctcgtgc gcgttcatca cgagccgctt cgcgtggccg gtcagcgaac cctgccacgc
     6121 gacgacgatc gcagctccgg cattctcgcc gacaacgcgg cccttgatga gctgtgccga
     6181 cgcgagcttc ggttcggtcg cgttgttcgc ctgctggatc cggtagagct ctttgtagtc
     6241 gcggcgcgcg aacgcgacct tcgcacgcac gtcgtcgagc tggggcagcc gcggcagctt
     6301 cgcggcttcg agggcgtcga ggccgcggcg cgcggcgaac agtgcgtcgt cgagtgcgcg
     6361 atcgtcgccg cgcatcagat agacctcggc ctcggtcgca gcggcgaacg ccgagcgctg
     6421 gacgtcgccg atctcgtcga actgcttgcg cgcttcggtg atgaggcgca gcgcctcgtc
     6481 gtagccagtc ggatagcgat tccattgcgc cgcgtgtgca taggcgaggg ccgcgagcag
     6541 cgagcgcgcg ccgaggagga gtgggtcgcc accggcggca ttcgtggcct tcgtgattcg
     6601 cacggtcagc tcgccgtgca gcggcgcttc ccgcgtcttg ggatccttca tcccgggcgg
     6661 tgcagccgcg tgtggattct cgagctcggc gagcgacgtg tcgagcaagc caagatacgc
     6721 cgagccttcg agccgcacgt cgcgtagctc ggggtcgcgc ggcaggctcg cgagtgcgcg
     6781 ctggtaggtc gcgcgtgcga gcgtcgtctc gccgaggcgc acgtattggc tccccgccgg
     6841 aaccagcacg agtggcgcga gcggcggcca gtcgagtggc ttcgcttcgg cgagcagcgc
     6901 gtcgatggca gcaccgagct gtggagccgg cacgccgcgc agcgcgaagc cacggccgat
     6961 gaggtgcagg atcttgtcgc ggcgaggctg atcgcgtgga acaccgggcg gcgcgagcgg
     7021 cgacgcggtt tcgcagctcg cgacggtagc cagcgcgtcg cgcggctcga agacgttcag
     7081 caccttggcg tcgacgtcgc cgagcatcgt cgtgatcgca gccgcctggt cgcgaatacc
     7141 ttcgagacac gcgatgcgtt ggcgcgcgat cttcgccggg cgctggcggc acgcctttgc
     7201 gtagctctcg acccagcggt cgcggtagcg gtcgagctgc tcgccgatgc gctcgacgtt
     7261 cgccggggtc gtcttgcgcg ccagcgcgct gcgaaccgca ggcgaccacg cctcgacgaa
     7321 ctgacgatcc ggatcgtcgc aaccagtctt cgagcggtca ccggcgcgca tcatgaacac
     7381 accgacgccg atcgcgacga ggagcgcgct cgcgacgagg gtgacgatca gccgcttgcg
     7441 tcgtgccggt tgtgtgcccg cgcgctcgag cgcgtcgagc agatcctcca tcgacgacca
     7501 gcgcgcactc gcggccggat ctaatccgcg cgtgagcacg gcgcgcactt cgctcggca
//
LOCUS       DASUFX010000704         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2057767, whole genome shotgun sequence.
ACCESSION   DASUFX010000704 DASUFX010000000
VERSION     DASUFX010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057767"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFV99_28415"
     CDS             complement(<1..951)
                     /locus_tag="VFV99_28415"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5063346.1"
                     /translation="MKGLGLQLPRATVGVSRWRRFRAAVDWPLILTIVALCAIGLLNL
                     YSATRGVRHQAKFEAQVQWMIVGAIGFVIMTVIDYRTLVRLAWGGLGIAIIMLIVARL
                     VGSTGDETHGVTYRWIHLPGVRLQPSELVKVMVILTLARVFADSNESSYTPRLLAIRL
                     GVFLIPIVLIAVQPDLGTAVLLTLLVLTVGYFAMPNVWPMVYITTAGLLAIPILWEKM
                     HDYQKNRILAFVDCSVAPSKLCWHTQQSVYAVGSGKIWGKGFLGGTQNQFNFVPEHWT
                     DFPYSVWAEEWGFAGSVALLAIFGFLLLWIINVALSARDRV"
     gene            complement(948..>2777)
                     /gene="mrdA"
                     /locus_tag="VFV99_28420"
     CDS             complement(948..>2777)
                     /gene="mrdA"
                     /locus_tag="VFV99_28420"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5063347.1"
                     /translation="ERYLPSVRGKILDTHGTALADNRPAFNIYATPSKLTDDIKAELA
                     RMLGLSDDEIAKIDERLALGKKRDPKEGILILEDQGRDRAALVEQARSRLPGVEVHHE
                     PYRYYPQGDLAAHLVGYMTQMTAEEADRLTAQGYDTSDLVGRYGLESAWENYLRGKRG
                     VEHYAVDARGQRLDNKTVADLIHGEGIVEPVPGANLILTIDSDLQRLAEKSVSHVAAA
                     AVVVVEVKTGRVRALVSKPSFDPNVMTGHLTHAEYTLLMQDPRKPFIDKTLRATYPPG
                     SIFKFVTTFAALEDGQAAEDESMFCTGEYELAGTRFRCHGTHEKVDLLKAIQKSCDVY
                     FWNLAQRIGLDRIAEVATSFGLGTPTNLGINGDSGGRIPTKAWYEKNGRYKIGFATNA
                     AIGQGDVEVTPMQMAMAYASLANNGSVYVPQVVERVESYDGRTIVSYDPKVARTVKVP
                     PDAMDTWKKGMWKVNNEPGGTAYDHGISQVVKVMGKTGTAEVKKHHKDSQNDKDLERW
                     NPNASHAWFAGWAPAEDPELVIVVMVEHGGAGGTIAWPIAERILDTYYFRKQKGDPKA
                     TPPPPIVPKKMPRDPRNDQEETRTRIPTRAMLKKQGERRKVEP"
BASE COUNT          432 a          912 c          932 g          501 t
ORIGIN      
        1 gacgcgatcg cgcgcggaca gcgccacgtt gatgatccac agcagcagga agccgaagat
       61 cgcgagcagc gcgaccgagc cggcgaagcc ccattcctcg gcccacaccg agtacgggaa
      121 gtcggtccag tgctcgggca cgaagttgaa ctggttctgc gtgccgccga ggaagccctt
      181 gccccagatc ttgccggaac cgaccgcgta gaccgactgc tgggtgtgcc agcacagctt
      241 gctcggcgcg acgctgcagt cgacgaacgc gaggatccgg ttcttctggt agtcgtgcat
      301 cttctcccag aggatcggaa tcgcgagcag gccggcggtc gtgatgtaga ccatcggcca
      361 cacgttcggc atcgcgaagt agccgacggt gagcacgagc agcgtgagca gaacggcggt
      421 gccgaggtcg ggctgcaccg cgatgagcac gatcggaatc aggaacacgc cgagtcggat
      481 cgcgagcagg cgcggcgtgt agctcgactc gttgctgtcg gcgaacacgc gcgcgagcgt
      541 gaggatgacc atcaccttca cgagctctga tggctgcagg cgcacgcccg gtaagtggat
      601 ccagcgatag gtgacgccgt gggtctcgtc gcctgtcgag ccgacgaggc gcgcgacgat
      661 cagcatgatg atcgcgatac cgagcccgcc ccacgcgagg cgaaccagcg tgcggtagtc
      721 gatcaccgtc atgatgacga agccgatcgc accgacgatc atccactgga cctgcgcctc
      781 gaacttggcc tgatggcgca cgccgcgggt cgcggagtac aggttgagaa ggccgatcgc
      841 gcacagcgcg acgatcgtga ggatcagcgg ccaatcgacc gcggcgcgga agcgacgcca
      901 gcgcgagacg ccgacggtgg cgcgcggcag ctgcaagccg aggcccttca cggctccacc
      961 ttcctgcgct cgccctgctt cttgagcatc gcgcgcgtcg gaatgcgcgt gcgtgtctcc
     1021 tcctgatcgt tgcgaggatc gcgcggcatc ttcttcggca cgatcggcgg cggcggtgtc
     1081 gccttcggat cgcccttctg cttgcggaag tagtacgtgt cgaggatgcg ctcggcgatc
     1141 ggccacgcga tcgtgccgcc cgcgccgcca tgctcgacca tcacgacgat cacgagctcg
     1201 ggatcttcag cgggcgccca gcccgcgaac cacgcgtgcg acgcgttcgg gttccagcgc
     1261 tcgaggtcct tgtcgttctg ggagtccttg tggtgcttct tgacctcggc ggtgccggtc
     1321 ttgcccatca ccttcacgac ctggctgatg ccgtggtcgt acgcggtgcc gccgggctcg
     1381 ttgttcacct tccacatgcc cttcttccag gtgtccatcg cgtcgggcgg caccttcacc
     1441 gtgcgcgcga ccttggggtc gtacgacacg atcgtgcggc cgtcgtacga ctcgactcgc
     1501 tcgacgacct gcggcacgta gaccgagcca ttgttcgcga gcgacgcgta ggccatcgcc
     1561 atctgcatcg gcgtgacctc gacgtcgccc tgcccgatcg ccgcgttcgt cgcgaagccg
     1621 atcttgtagc ggccgttctt ctcgtaccag gccttcgtcg gaatgcggcc gccggaatcg
     1681 ccgttgatgc cgaggttcgt cggtgtcccg agaccgaacg acgtcgcgac ctcggcgatg
     1741 cgatcgaggc cgatgcgctg cgcgaggttc cagaagtaca cgtcgcacga cttctggatc
     1801 gccttgagca agtcgacctt ctcgtgcgtg ccgtggcagc ggaaccgcgt acccgcgagc
     1861 tcgtactcgc cggtgcagaa catcgattcg tcctcggcgg cctggccgtc ttcgagcgcg
     1921 gcaaacgtcg tgacgaactt gaagatcgag cccggcggat acgtcgcgcg cagcgtcttg
     1981 tcgatgaacg gcttgcgcgg gtcctgcatc agcagcgtgt actcggcgtg cgtcaggtgg
     2041 ccggtcatca cgttcggatc gaacgacggc ttgctgacga gcgcgcgcac gcgcccggtc
     2101 ttcacttcga cgaccacgac tgccgctgcc gcaacgtgac tgaccgactt ctcggcgagc
     2161 cgctgcaggt cgctgtcgat cgtgaggatc aagttggcgc ccggcaccgg ctcgacgatg
     2221 ccttcgccgt ggatcaggtc ggcgacggtc ttgttgtcga ggcgttggcc gcgcgcgtcg
     2281 acggcgtaat gctcgacgcc gcgcttgccg cgcaggtagt tctcccacgc gctctcgagg
     2341 ccgtagcgac cgacgagatc gctcgtgtcg tagccctgcg cggtgagccg atcggcctcc
     2401 tccgcggtca tctgcgtcat gtagccgacg aggtgcgccg cgagatcgcc ctgcgggtag
     2461 tagcggtacg gctcgtgatg cacctcgaca ccgggcagtc gcgagcgcgc ttgctcgacg
     2521 agcgcggccc gatcgcgtcc ctgatcttcg aggatcagga tgccttcctt cgggtcgcgc
     2581 ttcttgccga gcgcgagccg ctcgtcgatc tttgcaatct cgtcgtcgga cagaccgagc
     2641 atgcgcgcga gctcggcctt gatgtcatcg gtgagcttcg agggcgtcgc gtagatgttg
     2701 aacgcaggcc ggttgtcggc gagcgcggtg ccgtgcgtgt cgaggatctt gccgcgcacg
     2761 ctcggcaggt agcgttc
//
LOCUS       DASUFX010000705         7393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1780425, whole genome shotgun sequence.
ACCESSION   DASUFX010000705 DASUFX010000000
VERSION     DASUFX010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780425"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="VFV99_28425"
     CDS             <1..1596
                     /locus_tag="VFV99_28425"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX5063348.1"
                     /translation="VGSATYVAANGELPFKLVPDGTYRFVDLSDGDGNFATIDYGEPG
                     DTPTLYVGQQLTLADLQIRGGEVGPPRDETIRSTRLACPNCNGPIELHSPDILRAACG
                     YCSTLLDVNAGALAVIGKVRKASPRIPLGTKAKFSEGEMTVIGYVERLAQVEGEWYPF
                     EEYLLYEPAVGFRWLVNSDGHWSYVQPVAVGAVEYQGEGVKYDGVKFRLFQGAQLRVD
                     TVLGEFYWKVEAGEEVWGEDFIAPPAMLSKETSNQEENWSLSTYLKPRELSAALGDKP
                     EFQFGGGTGVAPNQPDPAGAAATPLTLAFLALLVLGIVFAAIAPASEKYTSVAQIPAG
                     TFTPPPETEPATDVNPPNVFFSEPFELEGGKNVELGFRASLSNDWAYVVASLVNVASG
                     DVVTVDASMEYYSGVDDGESWSEGKEYDTEVVGPVTAGQYVLRLEMQHGATSGEVPLT
                     VRVRQGVFRGKYLYWSIFVLGIPFLILGIYSYSLERKRWSNSTQGTSSMPKTPLVIVV
                     GGIVLLLTGIVAIIKAIAESSSD"
     gene            1628..1831
                     /locus_tag="VFV99_28430"
     CDS             1628..1831
                     /locus_tag="VFV99_28430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063349.1"
                     /translation="MPRPKRSLLTAFALGFSVLVVGGYTALAITQRELGNEDRDAIPA
                     SVRSSPGGYRSYSFWHVGYHGGK"
     gene            1831..2052
                     /locus_tag="VFV99_28435"
     CDS             1831..2052
                     /locus_tag="VFV99_28435"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX5063350.1"
                     /translation="MKLDNLGEAAASSAIFAGIGLVVFGLAFWIMNKLAPFSIKKEIE
                     EDQNTALAIIMAGVIIGVSLIISSAIAGG"
     gene            2045..3574
                     /locus_tag="VFV99_28440"
     CDS             2045..3574
                     /locus_tag="VFV99_28440"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX5063351.1"
                     /translation="MADSAPPIRATALYLNVFVVAICGLVYELLAGTMGSYLLGDSVT
                     QFSLVIGLYLSAMGVGAWLSRRLDTELAKRFLEIELAVAVVGGASAPVLFIAFGSVVH
                     FQVVLLAFVFAIGVLVGLELPLLMRLLEGQVAFKDIVSRVLTFDYIGALAAALMFPLV
                     LVPHLGLVRSSLVMGSANAAVALWGTWLLAPRLGKRAIVSVRVRAILVLLGMLVALAF
                     ADVFTSWAEEEMFADPIVFSETTPYQRIVVTQNRAGFQLFLNGNLQFSSADEYRYHEA
                     LVHPAMASADAPPKKVLVLGGGDGLALREILAHPSVEQVTLVDLDPAMTGLSKKIPAL
                     AVLNRASFSDKRVTVINDDASVWIDEHPGLYDVIIVDFPDPNTFALGKLYTKSFYRRL
                     LAHLSPGGAVSIQATSPLFARKSYWCIVDTVQAAGFVVKPYQVTVPSFGVWGYVLARR
                     VPFEPPTDVPNVALRYLNASALRAMFEMPGDMTAVPVEINQLDNQALVRYYEREWRKY
                     E"
     gene            3556..5154
                     /locus_tag="VFV99_28445"
     CDS             3556..5154
                     /locus_tag="VFV99_28445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5063352.1"
                     /translation="MAEVRMTSTRRDFIAMMLGAPLAMACRGARPQPLPPGTLVDTGM
                     ARAHAAVRDGTVPTVTAWRQQHVVIVGGGVAGLGAAWELRRRGIRDVLVLELDDVVGG
                     TARGGSSSVTPYPWGAHYIVAPQRDQVDLTALLREMNAIEDVAADGTPIVDEQLRCRE
                     PEERVWYLGRWWDGLYLEAGANADDRKQLARFNAEIDRWSAWRDGQGRRAFTIPRSLG
                     SDDATVRALDQQSFAAWLDAHGLTSPRLRWLCDYACRDDYALTAAQTSAWAGIFYFAA
                     RQSAPGVDAQPVVTWPDGNGALVRHLATGATIERGVAVVDIDAAADGVRIMAVGPQGP
                     VGIRADAVIVAAPAFVANRIVRSRRGQRAGDYGAWAVANVHLRARPIERGRGAPPAWD
                     NVFRDSPSLGYVSATHQRGRDRGATVWTWYYPFTDEDSTSVRKKLAGAGYAEWADTVL
                     ADLARAHPDVRDHVDHIEVAFWGHGMVRPRIGSVWSDSLAARARPIGRIHFAHTDLSG
                     LAIFEEAFDHGLRAAREVAEVLRA"
     gene            5151..6212
                     /locus_tag="VFV99_28450"
     CDS             5151..6212
                     /locus_tag="VFV99_28450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063353.1"
                     /translation="MNPSSKWLFSAPLDLAVFGGTALVALVLVALAPALGIHNDTPEW
                     SWIMGVLLVDVAHVWSTTFVVYLDPVEWKRRPLLYALTPLAAFAIGVALYAWGPGPFW
                     RAIAYLAVYHFIRQQYGWVMMYRGRNGERDQLGRHLDGATIYLATIYPLVWWHVHLPR
                     GFAWMRTGDFLTGLPPWIADATGLVYLALLAIYVGRALAQLVRRQSVSWGKHLVVATT
                     AACWYVGIVATNTDYAFTITNVLIHGVPYMALVFIYARNAAREPGATSGANARLLRGA
                     RGIVIFLSTLWLIAYVEEMVWDRALWHDREWLFGSGIDVGTGALILAPLLAVPQLTHY
                     FLDGFLWRRRANPRLGRLL"
     gene            6227..>7393
                     /locus_tag="VFV99_28455"
     CDS             6227..>7393
                     /locus_tag="VFV99_28455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063354.1"
                     /translation="MGRSLLAFALVMAMVSVGAAQPPPPAADNEQAQFDAAFDAMVRG
                     DFGAASEGFKLLSVSALTPERRASSTELARLADELIARGGKLSFSTAPPGLPAAAPVA
                     PVTAPPNAVVAVSEDDAKDGGRASFVITTTMASFYSGFVLVDLLDTGDDARVSTGVVM
                     GATAAGVLGSVYGTKDRDMTGGMADAWGLGLFAGVGNALLLSEPLGLFDGTNASEHVN
                     LFVLGAGWGVATAGLLVADKIRPTRGQVTVVETFGLMGVSSTLLSLAILQPDNLDGDS
                     ALTVTAAGLDGGLAAGAIFGDKLDWSHSRARLVELSAFLGGLAGVGTSVMLLSDSGSD
                     DTFRAAAGITLAGLWGGFALGTHLTRNMQPDYRFRRGSTAMIAPTSIRNAPGLAL"
BASE COUNT         1108 a         2480 c         2460 g         1345 t
ORIGIN      
        1 gtcggtagcg cgacctatgt cgccgcgaac ggcgagctgc cattcaagct cgtgcccgac
       61 ggcacgtatc ggttcgtcga tctctcggac ggcgacggca acttcgcgac gatcgactac
      121 ggggagcccg gcgacacccc aacgttgtat gtcggccagc agctgacgct cgccgatctt
      181 cagatcagag gcggcgaggt cggtccgccg cgcgacgaga caatccgctc gacgcggctc
      241 gcgtgcccga actgcaacgg tccgatcgag ctgcactcgc ccgacattct gcgtgcggcg
      301 tgcgggtact gcagcacgtt gctcgacgtc aacgcgggcg cgctcgccgt catcggcaag
      361 gtgcgcaagg cgtcaccgcg gattccgctc ggtacgaaag cgaagttctc cgaaggcgag
      421 atgaccgtca tcggttacgt cgagcggctc gcccaggtcg aaggcgagtg gtacccgttc
      481 gaggagtact tgctgtacga gcctgcggtc ggatttcgct ggctcgtcaa ttccgatggc
      541 cactggagct acgtgcagcc agtcgcggtt ggcgccgtcg agtaccaggg tgagggcgtc
      601 aagtacgatg gcgtgaagtt ccggctgttc cagggcgcac agctgcgggt cgacaccgtg
      661 ctcggcgaat tctactggaa ggtcgaggcc ggcgaggaag tgtggggcga agacttcatc
      721 gcgccaccgg cgatgctgtc gaaggaaacg tcaaaccagg aagagaactg gtcgctgtcg
      781 acgtacctga agccgcgcga gctgtccgct gcgctcggcg acaagcccga gtttcagttc
      841 ggcggcggca ctggtgtcgc cccgaaccag cccgatcctg cgggcgctgc agcgacgccg
      901 ctcacgctcg cgttcctcgc attgctcgta ctcggcatcg tcttcgcggc gatcgcgccg
      961 gcgagcgaga agtacacgag cgtcgcgcag attccggccg gcacgttcac gccgccgccg
     1021 gagaccgagc ccgccacgga tgtgaacccg ccgaacgtat tcttctccga gccgttcgag
     1081 ctcgaaggcg gcaagaacgt cgagctcggc tttcgcgcca gcctgagcaa cgactgggcg
     1141 tacgtcgtcg cgtcactggt caacgtcgcg agcggcgacg tcgtcacggt cgacgcgagc
     1201 atggagtact actccggcgt cgacgacggc gaatcgtggt cggagggtaa ggagtacgat
     1261 accgaggtcg tcgggccggt gacggcaggc cagtacgtgt tgcgactcga gatgcagcac
     1321 ggcgcaacgt cgggcgaagt gccactgacc gttcgtgtgc ggcaaggcgt gttccgcggc
     1381 aagtacctct actggtcgat cttcgtgctc gggattccgt tcctcatcct cggcatctat
     1441 tcgtactcgc tcgagcggaa gcgctggtcg aacagcacgc agggcacgtc gagcatgccg
     1501 aagacaccgc tcgtgatcgt cgtcgggggc atcgtgctgt tgctcaccgg catcgtcgcc
     1561 atcatcaaag ccatcgcgga gtcatccagt gactgaagac gcaaaacgtc cacctgatcg
     1621 gccggtcgtg ccgcgcccga agcggtcgct gttgaccgcg ttcgcgctcg gcttctcggt
     1681 gctcgtcgtc ggtggctaca ccgcgctcgc gattacacag cgtgagctcg gcaatgaaga
     1741 tcgcgacgcg attccggcga gcgtgcggtc atcgccgggc ggctaccgct cctacagctt
     1801 ttggcacgtc ggttaccacg gagggaagtg atgaagctcg acaacctggg cgaagcagct
     1861 gcctcgtcag ccattttcgc aggcatcggc ctggtcgtat tcggcctcgc gttctggatc
     1921 atgaacaagc tggcgccgtt ctcgatcaag aaagagatcg aggaggatca gaacactgcg
     1981 ctggctatca tcatggccgg agtgatcatc ggtgtcagcc tcatcatctc ctctgccatc
     2041 gccggtggct gactcggcgc cgccgatccg cgcgaccgcg ttgtatctca acgtgttcgt
     2101 cgtcgcgatc tgcggccttg tctacgagct gctcgccggc acgatgggca gctatctgct
     2161 cggcgattcg gtcacccagt tctcgctcgt catcggtttg tatctgtcgg cgatgggcgt
     2221 tggcgcatgg ctatcgcgcc ggctcgacac cgagctcgcg aaacgctttc tcgagatcga
     2281 gctcgcggtc gcagtggtcg gcggcgcctc ggcgccggtg ttgttcatcg ccttcggctc
     2341 ggtcgttcac ttccaggtcg tgctgctcgc attcgtgttc gcgatcggcg tgctcgtcgg
     2401 tctcgaactg ccgctgttga tgcgactgct cgaagggcag gtcgcgttca aagacattgt
     2461 ctcgcgcgtg ctgacgttcg attacatcgg cgcgctcgcc gccgcgttga tgttcccgct
     2521 cgtgctcgtg ccccacctcg ggctcgtgcg gtcgtcgctc gtgatgggca gtgcgaacgc
     2581 agcggtcgcg ctatggggca cgtggctgct cgcgccgcgc ctcggcaagc gcgcgatcgt
     2641 cagcgtgcgc gtgcgcgcga tcctcgtgct gctcggcatg ctcgtcgcac ttgcgtttgc
     2701 cgacgtgttc acgtcatggg cggaggagga gatgttcgcc gacccgatcg tgttcagcga
     2761 gaccacgccg taccagcgga tcgttgtcac gcaaaaccgt gccggctttc agctgttcct
     2821 caacggcaac ctccagttct cgtcggcgga cgagtaccgg tatcacgaag cgctcgttca
     2881 tcccgccatg gcatccgccg acgcgccgcc gaagaaagtg ctcgtgctcg gtggcggcga
     2941 cggcctcgcg ttgcgcgaga tcctcgcgca cccatcggtc gagcaggtca cgctcgtcga
     3001 tctcgatccc gcgatgaccg gactgtcgaa gaagatcccc gcgctcgccg tgctcaaccg
     3061 cgcgtcgttc tccgacaagc gcgtcaccgt catcaacgat gacgcctcgg tgtggatcga
     3121 cgagcaccct gggctctacg acgtgatcat cgtcgacttc ccggacccga acacgttcgc
     3181 gctcggcaag ctctacacga agtcgttcta taggcgctta ctcgcgcatc tgtcgccggg
     3241 cggcgcggtc agcatccaag caacgtcgcc gctgttcgcg cgcaagtcgt actggtgcat
     3301 cgtcgacacg gtgcaggcgg caggcttcgt ggtgaagccg taccaggtca ccgtgccgtc
     3361 attcggcgtc tggggctacg tgctcgcgcg gcgcgtgccg ttcgaaccgc cgaccgacgt
     3421 gccaaacgtc gcgctccgct acctcaacgc gagcgcgctc agagcgatgt tcgagatgcc
     3481 gggcgacatg accgcggtgc cagtcgagat caaccagctc gataaccaag cgctcgttcg
     3541 ttactacgag cgcgaatggc ggaagtacga atgacctcca cgcggcgcga cttcatcgcg
     3601 atgatgctgg gcgcgccgct cgcgatggcg tgtcgtggtg cacggccgca accgctgccg
     3661 cccgggacgc tcgtcgacac cggaatggcg cgtgctcacg cagcagttcg cgacggaacg
     3721 gtgccaacag tcactgcgtg gcgccaacag cacgtcgtga tcgtcggtgg cggcgtcgcc
     3781 ggcctcggtg cggcgtggga gctgcggcga cgcggcattc gcgacgtgct cgtgctcgag
     3841 ctcgacgatg tcgttggtgg caccgcgcgc ggcggtagct cgagcgtgac gccgtatcca
     3901 tggggtgcgc actacatcgt cgcgccgcag cgcgaccagg tcgatctcac cgcgctgctc
     3961 cgcgagatga atgcgatcga ggacgtcgcc gccgatggca cgccgatcgt cgacgagcag
     4021 ctgcgctgcc gcgagcccga ggaacgcgtc tggtatctcg gccgctggtg ggacgggctc
     4081 tacctcgagg ccggtgcgaa cgcggacgat cgcaagcagc tcgcgcgctt caatgccgag
     4141 atcgaccgct ggtccgcgtg gcgcgacggg cagggcaggc gcgcattcac gattccgcgt
     4201 tcacttggca gcgacgacgc gacggtgcgc gcactcgatc aacaatcgtt cgccgcatgg
     4261 ctcgatgccc acggcctcac gtcgccccgg ttgcgctggc tatgcgatta cgcgtgccgt
     4321 gatgactacg cgctgaccgc ggcgcagacg agtgcatggg ccggcatctt ctatttcgct
     4381 gcccgccaga gcgcgcctgg cgtcgacgcg caaccggtcg tcacgtggcc cgacggcaac
     4441 ggcgcgctcg tgcgtcacct cgcgactgga gcgacgatcg agcgtggcgt cgctgtcgtc
     4501 gacatcgatg ccgcggccga cggcgtacgc atcatggcag ttggcccaca ggggccggtc
     4561 gggatccgcg ccgatgccgt gatcgtcgcg gcgccggcgt ttgtcgcgaa tcgcatcgtg
     4621 cgtagcaggc gcggccagcg agccggcgac tatggcgcat gggcggtggc gaacgttcat
     4681 ttgcgcgcgc gcccgatcga gcggggtcgc ggagcgccgc ctgcgtggga caacgtgttc
     4741 cgcgacagtc cgagcctcgg ctacgtgagc gcgacgcacc agcgcggccg cgatcgcggc
     4801 gcgaccgtgt ggacctggta ctacccgttc accgacgagg acagcacctc ggtgcgcaag
     4861 aagctcgccg gcgcaggcta cgccgagtgg gccgacaccg tgctcgccga tctcgcacgc
     4921 gcgcatcccg acgtccgcga tcacgtcgat cacatcgaag tcgcgttctg ggggcacggc
     4981 atggtgcgcc cgcgcatcgg ctcggtgtgg agcgattcgc ttgcggctcg tgcacggccg
     5041 atcggccgca ttcatttcgc gcacaccgat ctatccggcc ttgcaatctt cgaggaggcg
     5101 ttcgatcacg ggctgcgcgc ggcgcgcgaa gtcgcggagg tcctgcgcgc atgaatccga
     5161 gcagcaagtg gctgttctcg gcgccgctcg atctcgccgt gttcggcggc accgcgctcg
     5221 tcgcgctcgt gctcgtggcg ctcgcgcccg cgctcggcat ccacaacgac acgcccgagt
     5281 ggtcatggat catgggcgtg ctgcttgtcg acgtcgcgca cgtctggtcg acgacgttcg
     5341 tcgtgtacct cgatcccgtc gagtggaagc gccggccgct gctctacgcg ctcacgccgc
     5401 tcgccgcgtt cgcgatcggc gtcgcgctct acgcgtgggg gccaggtccg ttctggcgcg
     5461 cgatcgcgta tctcgcggtc tatcacttca ttcgccagca gtacggctgg gtgatgatgt
     5521 accgcggccg caacggcgag cgcgatcagc tcggccgcca cctcgacggc gccacgatct
     5581 acctcgcgac gatctatccg ctcgtctggt ggcacgtgca tctaccgcgg ggcttcgcgt
     5641 ggatgcgcac cggcgatttc ctgaccggcc tgccgccgtg gatcgcggac gcgaccggcc
     5701 tcgtctacct cgcgctgctc gcgatctacg tcggtcgcgc gctcgcacag ctcgtgcgcc
     5761 gccagtcggt ctcgtggggc aagcacctcg tcgtcgccac gaccgccgcc tgctggtacg
     5821 tcggtatcgt agccacgaac accgactacg cgttcacgat cacgaacgtg ctgatccatg
     5881 gcgtgccgta catggcgctg gtgttcatct atgcgcgcaa cgccgcacgc gaaccgggcg
     5941 cgaccagcgg cgcgaatgcg cgactgcttc gcggcgctcg cggcatcgtg atctttctct
     6001 cgacgctctg gctgattgcc tacgtcgagg agatggtgtg ggaccgagca ctgtggcacg
     6061 accgcgagtg gctattcggc tccgggatcg acgtcggcac cggcgcgctc atcctcgcac
     6121 cgctcctcgc agtgccacag cttacccact atttcctcga cggtttcctg tggcgccgcc
     6181 gtgcgaaccc gcgcctgggc cggttgcttt gacatagact ggagccatgg ggcgtagcct
     6241 tcttgcgttc gcgttggtga tggcaatggt ttcggtcggc gccgcgcagc caccaccgcc
     6301 agccgctgac aacgagcaag cgcagttcga cgctgccttc gacgcgatgg tgcgcggaga
     6361 cttcggggcc gcgtcggagg gcttcaagct gctctcggtc agtgctctca ccccggagcg
     6421 ccgtgcatcg tccaccgagc tcgctcgcct agccgacgag ctgatcgcac gcggcggtaa
     6481 gctgtcgttc agcacggcgc cgcccggcct tcctgctgcc gcaccggttg ccccggtcac
     6541 tgcgccgccg aatgcggtcg tcgcggtctc cgaagacgac gcgaaagacg gtggtcgcgc
     6601 gagcttcgtc atcacgacga cgatggcctc gttctacagc ggcttcgtgc tcgtcgatct
     6661 gctcgacacc ggcgacgacg cccgcgtcag caccggtgtc gtgatgggcg caaccgccgc
     6721 cggcgtgctc ggctccgtct atggcacgaa ggaccgtgac atgaccggtg gcatggcgga
     6781 cgcgtggggg ctcggtctgt tcgccggcgt cggcaacgcg ctgctgctgt cggagccact
     6841 cggcctgttc gacggcacga acgcgagcga gcatgtgaac ctgttcgtgc tcggggccgg
     6901 ttggggcgtc gcaacagccg gcttgctcgt cgccgacaag atccggccga cgcgcggcca
     6961 ggtcaccgtc gtcgagacat tcggcttgat gggggtctcg tcgacgctgc tgtcgctcgc
     7021 gatcctccag cccgacaacc tcgacggcga cagtgccctc actgttacgg cggccggtct
     7081 cgatggcggt ctcgccgccg gcgcgatctt cggcgacaag ctcgattggt cgcactcgcg
     7141 tgcgcgcctc gtcgagctga gcgcgttcct cggcgggctc gccggcgtcg gcacgtcagt
     7201 catgctgctt tctgatagcg gcagtgacga cacctttcga gccgccgcgg gcatcacgct
     7261 cgccggcttg tggggcggct tcgcgctcgg cacgcacctg acccgcaaca tgcagccgga
     7321 ctaccgcttc cgccgcggct cgaccgcgat gatcgcgccg acgtcgatcc gcaacgcgcc
     7381 cggccttgca ctg
//
LOCUS       DASUFX010000706         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_390515,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000706 DASUFX010000000
VERSION     DASUFX010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390515"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFV99_28460"
     CDS             complement(<1..492)
                     /locus_tag="VFV99_28460"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5063355.1"
                     /translation="MSRSELGEAALNGDLERLQQLIAQGALLDGSDGELYNDDTPGCV
                     RGAPYYRPLGEAARMNHPECVRALLAAGAGLEEQNYTGETALIQAVRQDAIDCVKILL
                     DAGADVHHVACLGTALAATQPGYYPNAIEIARLLIERGASPTTTASYARSALERWKDQ
                     IPTR"
     gene            complement(517..1353)
                     /gene="fdhD"
                     /locus_tag="VFV99_28465"
     CDS             complement(517..1353)
                     /gene="fdhD"
                     /locus_tag="VFV99_28465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5063356.1"
                     /translation="MIGCETGSDCDSTPIARALYNLTVTTTRDIYVERIDATVRKSDT
                     DRVVVEAPLQIRARGVPVATVMRTPGHDLELVRGLLHAESVAAGALAQVDEDAIDVDV
                     DATAFAGRGLLASAACGVCGRVAIADLELRAQSVTADTSIDSGVLAKLPDTLRAAQDV
                     FGDTGGLHAAGLFSTSGALIASREDVGRHNAVDKLVGWALAETTRGKLVDPSKHVLFL
                     SGRAGYELVQKAIMFGVPIVASVSAPSSLAIELAERFNVALAGFVRGSRCNIYSHGWR
                     IP"
     gene            complement(1322..1945)
                     /locus_tag="VFV99_28470"
     CDS             complement(1322..1945)
                     /locus_tag="VFV99_28470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063357.1"
                     /translation="MKRDMAFVLDVGWNNAEDAQRSRRAHFQAPEPRREVRRYADLDR
                     TVGARLNEVGHRTGLVAEQRLLDELDSFEVREQAVPPIPHRFELAVAPRRECSQDFDR
                     DCRCAPTAHDANEHQYALLREGVRWVPESTTASTLRRAVSDVAPLVQRELKRKIRRKP
                     LAVSLHVLIELFRRDPIKPGEVTVENDLFTPDAVQSQRDRLRDWIRL"
     gene            2049..3347
                     /locus_tag="VFV99_28475"
     CDS             2049..3347
                     /locus_tag="VFV99_28475"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5063358.1"
                     /translation="MKDRVFRDPVHGLIEFKGADRPIADLLDTLAMQRLRRIKQMGFA
                     WLVYPGAEHSRFGHALGAFHIAQRVTRRLDLDPRVALHVKVAALLHDVGHGPFSHAWE
                     HVFAGHDHERWGARIATEHAELRAVLEQLEPGLPETLKSFWGKGYKPHFARKLVSSQL
                     DVDRLDYLLRDGHYSGAGYATYDLDWIIHAIQIAPVRSGGDDPSDLVVDYRRGMYAVE
                     QYLFARSYMYAQVYHHKTVRAAEWMFIKTLERFAELARAGAEPVGLAIAGSMARGGGV
                     GVDEYLRLHDVSLTTAMDRWAGIDGNPPADDPVLRDLATRLVTRRLFKTFDLGDDRPA
                     AERLMPSAIEVAEKRFGAHARFYVHLDTARHAAYLASDDEELHVIDHPKYGAVTLSRL
                     LEDMPLGQPTATIRLVCAPELVDDLRPLVDHVLGRHRGRR"
BASE COUNT          586 a         1199 c         1209 g          614 t
ORIGIN      
        1 gcgcgtcgga atctgatcct tccagcgctc gagtgcactg cgcgcgtacg atgccgtcgt
       61 cgtcggcgag gcgccgcgct cgatcagcag tctggcgatc tcgatcgcgt tcgggtagta
      121 gcccggctgc gtcgcggcga gtgccgtgcc gaggcacgcg acgtgatgca cgtcagcgcc
      181 agcatcgagc aggatcttga cgcagtcgat cgcgtcctga cgcaccgcct ggatcagagc
      241 ggtctcgccg gtgtagttct gctcctcgag gcccgcgcca gccgcgagca atgcgcgtac
      301 gcactcgggg tggttcatgc gtgctgcttc gccgagcggc cggtagtacg gcgcgccgcg
      361 cacgcagccg ggcgtgtcgt cgttgtagag ctcgccatcg ctgccgtcga gcaacgcgcc
      421 ttgtgcgatc aactgctgca gccgctccag gtcaccgttg agcgctgctt ccccgagctc
      481 ggaacgtgac atgcggcgat tgtagcgaac cagcgctcag ggaatgcgcc agccgtgact
      541 atagatgttg caacgagatc cgcgcacgaa gccggcgagc gcgacgttga accgctcggc
      601 gagctcgatc gcgagcgacg acggtgcgga tacgctcgcg acgatcggca cgccaaacat
      661 gatggccttc tggacaagct cgtagccggc gcggccgctg aggaacagca cgtgcttgct
      721 cggatcgacg agcttgccgc gcgtggtctc ggcgagcgcc cagccgacga gcttgtcgac
      781 cgcgttgtgg cgcccgacat cctcgcgcga cgcgatcagc gcgccgctcg tcgaaaacag
      841 acccgcggca tgaaggccac cggtatcgcc aaacacgtcc tgcgccgcgc gcagcgtgtc
      901 gggcagcttc gcgagcacgc cgctgtcgat cgaggtgtct gcggtgaccg actgcgcgcg
      961 caactctaga tccgcgatcg cgacgcggcc gcacacgccg cacgccgcac tcgcgagcag
     1021 cccgcgcccc gcaaacgcgg tcgcgtcgac gtcgacatcg atcgcgtcct cgtcgacctg
     1081 cgcgagcgcg cccgcggcaa cggactctgc gtgcaggaga ccgcgcacga gctcgaggtc
     1141 gtgccccggc gtacgcatca ccgtcgcgac cggcacgccg cgcgcgcgaa tctgcagcgg
     1201 tgcctcgacg acgacgcgat cggtgtccga ttttcggaca gtcgcatcga tgcgttcgac
     1261 atagatatcg cgggtcgttg tcacggtgag gttgtatagt gcgcgagcga tgggcgtcga
     1321 gtcacaatcg gatccagtct cgcaaccgat cacgctgcga ttgcaccgcg tccggggtga
     1381 acaggtcatt ctcgacggtg acctcgcccg gctttatggg atcccgacga aacagctcaa
     1441 tcagaacgtg aagcgacaca gcgagcggtt tccgccggat tttgcgtttc agctcacgct
     1501 ggacgagtgg cgctacgtca gagacggcgc gccgaagcgt cgaggccgtg gtggactcgg
     1561 gcacccaccg tacgccttca cggagcaggg cgtactgatg ctcgttggca tcatgtgcgg
     1621 taggcgcgca acggcaatct cgatcgaaat cctgcgagca ttcgcggcga ggcgcgacgg
     1681 cgagctcgaa tcggtgtggg attgggggaa cggcctgttc acggacctcg aacgaatcga
     1741 gctcgtcgag aaggcgttgt tcggcgacga gcccggtacg gtgacctact tcattcaatc
     1801 gggcaccaac ggtccgatca agatcggcgt aacgacgaac ttctcgtcgc ggttccggag
     1861 cctggaaatg agctcgccta ctccgttgcg cgtcctcggc gttgttccaa ccgacatcga
     1921 ggacgaatgc catgtcgcgc ttcacgaatg gcgtctgcac ggtgaatggt tcaaacccga
     1981 cgcggaggtt cttacgttta ttcggcgcag gctccccatg aggcgaagtg tcaattagga
     2041 tgccgcccgt gaaagaccgc gtgttccgcg atccagtgca tggactcatc gagttcaaag
     2101 gcgcggatcg gccgatcgcg gacctgctcg acacgctcgc aatgcagcgc ctgcgccgca
     2161 tcaagcagat gggcttcgcg tggctcgtgt atccgggcgc cgagcactcg cgcttcggtc
     2221 atgcgctcgg cgcgtttcac atcgcccagc gcgtgacgcg ccggctcgat ctcgatccgc
     2281 gtgtcgcgct acacgtgaaa gtcgcggcgc tcttgcacga cgtcggccac ggcccgttct
     2341 cgcatgcgtg ggagcacgtg ttcgccggcc acgatcacga gcgctggggc gctcgcattg
     2401 cgaccgagca cgccgagctg cgcgcggtgc tcgaacagct cgagccgggc ttgcccgaga
     2461 cgttgaaatc gttctggggc aagggctaca agccgcactt cgcgcgcaag ctggtgtcgt
     2521 cgcagctcga cgtcgaccgg ctcgattatt tgctccgcga tggtcattac tccggcgcgg
     2581 gctacgcaac gtacgacctc gactggatca tccacgcgat ccagatcgcg cccgtgcgca
     2641 gcggcggcga cgatccgtcg gacctcgtcg tcgactaccg gcgcggcatg tacgccgtcg
     2701 agcagtacct gttcgcgcgt tcgtacatgt acgcgcaggt ctatcaccac aagaccgtgc
     2761 gcgcggcgga gtggatgttc atcaagacgc tcgagcgctt cgccgagctc gcccgcgcag
     2821 gtgccgagcc ggtcgggctc gcgatcgccg gctcgatggc ccgcggcggc ggcgtcggcg
     2881 tcgacgagta tctgcggctg cacgacgtgt ccttgacgac ggcgatggat cgctgggccg
     2941 gcatcgacgg caatccgccc gccgatgatc cggttctgcg cgatctggcg acgcggctcg
     3001 tgacgcggcg gttgttcaag acgttcgacc tcggcgatga ccgcccggcg gcggagcgac
     3061 tgatgccctc ggcgatcgaa gttgccgaga agcgcttcgg cgcgcacgcg cggttctacg
     3121 tccacctcga caccgcgcgg cacgcggcgt atctcgcgtc cgacgacgaa gagctgcacg
     3181 tcatcgatca cccgaagtac ggcgcggtga cactgtcgcg cttgctcgag gacatgccac
     3241 tcggtcaacc gaccgcgacg atccggctcg tgtgtgcgcc cgagctcgtc gacgatctgc
     3301 ggccactcgt cgatcacgtg ctcggccggc atcgcggccg gcgctagcgg gcgccaagcg
     3361 gacgcgagcg gcggtcggac caacgcgacg cgtgacgcca gtcgcttcgg cggcggtggc
     3421 gtgtaaccga aacgtaggaa acgctgctca gcggcgagcg catagtccgc gccgtgaccc
     3481 ggggtgtctc gctcgttttc gcgtgtggat cgctgtgcgc gtgcagcgct ccgtcggcgg
     3541 ccatcctcga gcccgacgct gccgttgtca tcgagcccga tgcgcccgcg ccggtcgatc
     3601 acgcgccg
//
LOCUS       DASUFX010000707         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_947295,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000707 DASUFX010000000
VERSION     DASUFX010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947295"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VFV99_28480"
     CDS             <1..1270
                     /locus_tag="VFV99_28480"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEX5063359.1"
                     /translation="TSSADAPADAEIDAAIDAPPDAPPDACVANAELCNAHDDDCDGH
                     VDEGYSTGLPCDGADADSCAEGFVVCSPMGGTMCSDMTGNNVELCNGLDDDCKNGVDD
                     AFPVGQPCSVGLGACARSGQLMCNSTQTGTVCNASAGAPSAETCGNAIDEDCNGADAV
                     CPGNDSAAGAFDISAGGTFTVDLTAAHDDNWAASTATLDCGDQGGRDAFYQFTLPAEE
                     VVYYDSFGSNFDTVIRIYAGACTSLGAVKACADDACSTTRSQGALDLLAGTYCLVLDQ
                     FSSTTTAGSASLTFKRGGRSGVGIATASGSQTGTTTGKTNVSIAGCEANSAQPDVGYF
                     FLSCPSHTYTVSASTCSGTAFDSVIYLRSGAATTGDVACSDDESGCGTSGFQSRFTGA
                     TVTGANINWLIVDGFGQTGNGNYTLTYTVQ"
     gene            1294..2151
                     /locus_tag="VFV99_28485"
     CDS             1294..2151
                     /locus_tag="VFV99_28485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063360.1"
                     /translation="MRLGKYTLVAPLGRGGMGGVWEIKDDAGNAYALKSPAGGMNPAA
                     EATKRFAREANALRMLDHPNLVAAVDVFVEAGYLFLVMEYVRGRTLTKVMQAEGPIQA
                     RRALVIARQLLDGVGHAHAQGLVHRDLKPDNIMLVDMGGWERAKIVDFGLVKLIGDAE
                     AAMGGGKLTSTGLVFGTPAYMAPEQALGKLVDGRADLYAAATILFEMLTGRVPFDHAD
                     PMMQMKMQVKAPPPRLDMLSKGAAWCTPQLSALIDAALAKDPQHRFPNAQVMMSALED
                     AFVSLDHIP"
     gene            complement(2152..>2405)
                     /locus_tag="VFV99_28490"
     CDS             complement(2152..>2405)
                     /locus_tag="VFV99_28490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063361.1"
                     /translation="TPTFAPPVAGPPLDHVEVHYPPNLTGKSGRDFEKVVDKIYKNDF
                     DAALRELDKFEDKYGQREETIDLRDQLNRVPRQPERDDE"
BASE COUNT          422 a          761 c          830 g          392 t
ORIGIN      
        1 cacgtcatca gcggatgcgc ccgccgacgc cgagatcgac gcagcgatcg atgcaccacc
       61 ggacgcgccg cctgatgcat gcgtcgcgaa cgcagagctg tgcaatgcgc acgacgacga
      121 ctgcgacggt cacgtcgatg agggctacag cacgggtttg ccgtgcgatg gcgccgatgc
      181 cgactcgtgt gcggaaggct tcgtcgtgtg cagcccgatg ggcggcacga tgtgcagcga
      241 catgacgggc aacaacgtcg agctgtgcaa cggtctcgac gacgactgca agaacggcgt
      301 cgacgacgcg tttcccgtcg gtcaaccatg ctcggtcggc ctcggcgcgt gcgcgcgctc
      361 ggggcagctg atgtgcaaca gcacgcagac cggcaccgtc tgcaatgcga gcgcaggcgc
      421 gccgagtgcg gagacgtgcg gcaacgcgat cgacgaagac tgcaacggtg ccgatgcggt
      481 gtgcccgggc aacgacagtg ccgcgggcgc gttcgacatc tcggccggcg gcacgttcac
      541 ggtcgatctc acggcggcgc acgacgacaa ctgggcggcg tcgaccgcga cgctcgactg
      601 cggcgatcag ggcgggcgcg acgcgttcta tcagttcacg ctgccggcgg aggaagtcgt
      661 ctactacgac tcgttcggct cgaacttcga cacggtgatc cgcatctacg cgggtgcatg
      721 cacgtcgctc ggtgcggtca aggcgtgcgc cgacgacgcc tgctcgacga cgcgctcgca
      781 aggcgcgctc gacctgctcg ccggtacgta ttgcctcgtg ctcgaccagt tctcgtcgac
      841 gacaaccgca ggatccgcat cgctcacgtt caagcgcggc ggtcgcagcg gcgtcgggat
      901 cgccaccgcg agcggctcgc agaccggtac gacgaccggc aagacgaacg tctcgatcgc
      961 cgggtgcgag gcgaactccg cgcagcccga cgtcggctac ttcttcctct cgtgcccgag
     1021 ccacacgtac accgtgtcgg cgagcacgtg cagcggcacc gcgttcgaca gcgtgatcta
     1081 tttacggagc ggtgcggcaa cgaccggcga cgtcgcatgt agcgatgacg agagcggctg
     1141 tggcacgagc ggcttccagt cgcggttcac gggagcgacg gtcacaggcg ctaacatcaa
     1201 ttggctgatc gttgatggct tcggccagac cggaaacggc aattacacgc taacgtatac
     1261 cgtgcagtga gcgcaccgca gttcgagccg gggatgaggc tcgggaagta cacgctcgtc
     1321 gcaccgttgg ggcgcggcgg catgggcggc gtgtgggaaa tcaaagacga cgcgggcaac
     1381 gcgtacgcgc tgaagtcgcc ggcgggcggg atgaacccgg cggccgaagc gacgaagcga
     1441 ttcgcgcgcg aagcgaacgc gctgcgcatg ctcgatcatc ccaacctcgt ggcggccgtc
     1501 gacgtgttcg tcgaggctgg ctacctgttc ctcgtcatgg agtacgtgcg cgggcgaacg
     1561 ctcacgaaag tgatgcaggc cgaggggccg atacaggcgc gccgcgcgct cgtgatcgct
     1621 cgccagctgc tcgacggcgt cggccacgct cacgcgcagg ggctcgtgca tcgtgacctc
     1681 aagcccgaca acatcatgct cgtcgacatg ggtgggtggg agcgcgcgaa gatcgtcgac
     1741 ttcggtctcg tgaagctgat cggcgacgcc gaggcagcga tgggcggcgg caaactgacg
     1801 tcgacagggc tcgtgttcgg cacgcccgcg tacatggcgc cggaacaagc gctcggcaag
     1861 ctcgtcgacg gccgcgccga tctctacgcc gccgcgacga tcctgttcga gatgctcacc
     1921 gggcgcgtgc cattcgatca cgccgatccg atgatgcaga tgaagatgca ggtgaaggcg
     1981 ccgccgccgc gcctcgatat gctgtcgaag ggcgcagcgt ggtgcacgcc tcagctctcg
     2041 gcgctgatcg atgccgcgct cgcaaaggat ccgcagcatc ggtttccgaa cgcgcaggtg
     2101 atgatgtcgg cgctcgagga cgcgttcgtg tcgctcgatc acattccgtg atcactcgtc
     2161 gtcgcgctcg ggctgacgcg ggacgcgatt gagctgatcg cgcagatcga tcgtctcctc
     2221 gcgctggccg tacttgtctt cgaacttgtc gagctcgcga agagccgcat cgaaatcgtt
     2281 cttgtaaatc ttgtcgacga ccttctcgaa gtcgcgaccc gacttgccgg tgagattcgg
     2341 cgggtagtgc acttcgacat gatcgagtgg tgggcctgcg accggaggtg cgaacgtggg
     2401 tgtcg
//
LOCUS       DASUFX010000708         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1918435, whole genome shotgun sequence.
ACCESSION   DASUFX010000708 DASUFX010000000
VERSION     DASUFX010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918435"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>2255)
                     /locus_tag="VFV99_28495"
     CDS             complement(177..>2255)
                     /locus_tag="VFV99_28495"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5063362.1"
                     /translation="PAARTVDVVDKQFGIEAPDPYRWMEGTDNAEYTEWLRTQGEYTA
                     KQLAAIPGRDKLHARLRELGLGVTAVFDVQLGGKRIFHKLFPANAQLAKLAVRDPDGK
                     TRVLVDPEAFGGETVALNAYSASPDGAYVAYVISKGGGERGVLHIMDAATGKDLPDQI
                     ERIWGEGAAAWLPDSKRFFYTQLAVPQPGVDPMINQTVRFHVVGKPVDADATIVGRDA
                     NASWKLAPEEWPFIATPSGSKWAVLYVGGARNELRIGVAKISELDLSGAGKTPWRAVG
                     DYNDVLEWGIPHGDRLYLQTAKDAPNGRVISVLLAKPDLKQARVEIAEDPNAKLSGMY
                     PARDALYVLHQTNGLARVSRWAWQGKASPLALPYEGWTPDLASDMTKDGITFQIEGWL
                     RLGTYYAYDPAKKQLTPTGLASSSANADVSQIVAEEVEATSADGTKVPLSILHRKDVT
                     LDGSHPALLYGYAGYGASETPGFSASRVVWLERGGVYATCHGRGGGEKGRRWQNDGSR
                     EKKLNGVRDFIACAEYLVDKKFTSPAKLAALGISMGGVLVGRALTERPDLFGAIQLSV
                     GIVNPLRILAAENGANQKGELGDPTTEAGYRSIFDMDPYQHVKPNTAYPAVIFTIGLN
                     DHRVAPWMTGKMASRLRTATTSKRPILIRVDANAGHGVGSTREQGFAERADAWSFLLQ
                     ALGDPDFAPR"
BASE COUNT          366 a          745 c          738 g          406 t
ORIGIN      
        1 tgttttggat cggtctggag ttgcagcttg taagacgtaa agtcttccgc gaccgccggc
       61 cctgctacca ccaacaacgc aactgtcagc accactctcg agaacaacgg agtgagcacg
      121 atgccatcgt cgcacgaccg ccgggcgaac gcgatggacg gtgcgcgtag aagcgactaa
      181 cgcggggcga agtcgggatc gccgagcgcc tgcagcaaga agctccatgc gtcggcacgc
      241 tcggcgaagc cttgctcgcg cgtcgaaccg acgccgtgtc cagcgttggc gtcgacgcgg
      301 atcaagatcg gccgcttgct cgttgtcgcg gtgcgcaggc gcgatgccat cttgcccgtc
      361 atccacggcg cgacgcggtg atcgttgaga ccgatcgtga agatgaccgc cgggtacgcc
      421 gtgttgggtt tgacgtgctg gtacggatcc atgtcgaaga tcgatcgata gccagcctcg
      481 gtggtcggat caccgagctc gcccttctgg ttcgcgccgt tttcggccgc gaggatgcgg
      541 agcgggttga cgatgccgac cgagagctgg atcgcgccga acagatccgg ccgctcggtc
      601 agcgcgcgac cgacgaggac gccgcccatg ctgatgccga gtgcggcgag cttggcgggc
      661 gacgtgaatt tcttgtcgac gaggtactcg gcgcacgcga tgaagtcccg cacaccgttg
      721 agcttcttct cgcgcgagcc atcgttctgc cagcgccggc ctttctcacc accaccccgg
      781 ccgtggcagg tcgcgtacac gccaccgcgt tcgagccaca cgacgcgact tgccgagaag
      841 cccggcgttt ccgaggcacc gtaaccggcg tagccgtaga gcagtgccgg atgcgagcca
      901 tcgagcgtga cgtccttgcg atgcaggatc gagagcggca ccttcgtgcc gtcggcgctc
      961 gtcgcttcga cttcctcggc gacaatttgc gagacgtccg cgttcgccga tgacgacgcg
     1021 agtcccgtcg gcgtgagttg tttcttcgcc ggatcgtacg cgtagtacgt gccgagccgc
     1081 agccagcctt cgatctggaa cgtgatgccg tccttcgtca tgtccgacgc gaggtcgggc
     1141 gtccagcctt cgtatgggag cgcgagcggc gacgctttgc cttgccacgc ccagcgcgac
     1201 acgcgcgcta gcccgttcgt ctggtggagc acgtagagcg cgtcgcgtgc gggatacatg
     1261 cccgacagct tcgcgttcgg atcttctgcg atctcgacgc gcgcttgctt caagtcgggc
     1321 ttggcgagca ggaccgagat gacgcggccg ttcggtgcgt ccttcgcggt ctgcagatac
     1381 aggcgatcac cgtgcgggat tccccactcg agcacgtcgt tgtaatcgcc gaccgcgcgc
     1441 cacggcgtct tgcccgcgcc ggagagatcg agctcgctga tcttcgcgac accgatccgc
     1501 agctcgttac gcgcaccacc gacgtagagg accgcccatt tcgatcccga cggcgtcgcg
     1561 atgaacggcc actcctcggg cgccagcttc cacgatgcat tcgcgtcgcg gccgacgatg
     1621 gtcgcgtcgg cgtcgacagg cttaccgacc acgtggaagc gcaccgtctg gttgatcatc
     1681 ggatcgacgc cgggctgcgg caccgcaagc tgcgtgtaga agaagcgctt gctatcgggc
     1741 aaccaggccg ctgcgccttc gccccagatg cgctcgatct gatcggggag gtccttaccg
     1801 gtcgccgcat ccatgatgtg gagcacgccg cgctcgccgc cacccttgga gatcacgtag
     1861 gcgacgtagg cgccatccgg cgacgccgag tacgcattga gcgcgacggt ctcgccgccg
     1921 aatgcttcgg gatcgacgag cacgcgcgtc ttgccgtccg gatcgcggac cgcaagcttt
     1981 gcgagctgcg cgttcgcggg gaacagcttg tgaaagatcc gcttgccgcc aagctggacg
     2041 tcgaacaccg cggtgacgcc gaggccgagc tcgcgtagcc gcgcgtgaag cttgtcgcga
     2101 ccaggaatcg ccgcgagctg cttcgcggtg tactcgcctt gcgtgcgaag ccactcggtg
     2161 tactcggcgt tgtccgtgcc ttccatccaa cgatacggat ccggcgcctc gatcccgaac
     2221 tgtttgtcga cgacatcgac ggtccgcgcg gccgg
//
LOCUS       DASUFX010000709         9510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_253766,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000709 DASUFX010000000
VERSION     DASUFX010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253766"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2363
                     /locus_tag="VFV99_28500"
     CDS             <1..2363
                     /locus_tag="VFV99_28500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063363.1"
                     /translation="LARWRSEDAADRALLADVGAAARRWNESGRPADLLWRGQALAEL
                     TRLTKRSTALTELERAFATDAASAQQRARRIRRGIVAGVMIVLAAAASVMAYLSVVAN
                     ENRAEAERNATAAKQSAALADERLTASLIAQGRRELNDNRAMPALAYFAAAMKRGADT
                     AGLRAMVSIASRGWKDTLVTSHAAPFSSVTGSPNGWIAAGDQVGTVHYWSDTGVSLGE
                     VKTEVGPIATVERQPDDTLVVIGNTGVVLLGTKHEVLRRVQTEKSWYARFGPGPDELT
                     AVGDGKLRIYGFDGKVRREIALDTTASGVEPVFAPSMSHALVNVGDDIAKLDLVTMRQ
                     TIIVKNGWGPPGLSRTLSTYAYVDKDHRVHVIDLDGKPIKTLDVKSRTYGIVFSQDGD
                     RIGVVGDAYMTILDAKGERLGEYPIARDQSLFLLRGDHAWITGNQGIVRHYSDGELVS
                     SIPAFAVEVQASAVARDAVALIGSDASLAIIRANSAQIVEDKEVCDKPAYASNGIATG
                     YECAGHVKLYLGRSYIAEYPVSEMQLAVAYDEASHRSAVSGGAGVHIFDRDAKVIAKT
                     AREGRAAFEDGDHVLVAEAKKALWRWTISTDTWQELMPIGDIYALAVLPGKILLGQQD
                     GKLLVIENGHETHRADVGTQIGDIVASPDKRWLAVSLATGAVAIVDAQSWQVTRMLAA
                     ADNYGMAATFDGTGDLLIRSSRNALTIWDRASGEELLFGFDLLQDLSNGRFLPDGRIE
                     IDRRSPGLLDIPRDTRPIAQLVRDIDCKVPLKVVGSRIEPQIPSCP"
     gene            2401..3729
                     /locus_tag="VFV99_28505"
     CDS             2401..3729
                     /locus_tag="VFV99_28505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063364.1"
                     /translation="MIDDDVGATTEQIDRQAPFGDRVLLVIDGASLQVVPLPGTGTLT
                     IGRSQKCDVAIDSGSVSRHHANLLVSQDVNIEDVGSSNGTFVDGQRLDANKHVRIGIG
                     IPFLIGGVTVMVQTRAGARPAPASKTSKLAALEQSAARIAVGKLAVLILGETGVGKER
                     FAERLHEMSPRRPAPFVRINCAAISEPLLEAELFGNEGTNKPGLIEHADGGTAFLNDV
                     SELPQSLQVKLLRVLEDNQVRRIGSANPRTVDVRFIAATNKDMQAEVDAGRFRRDLYF
                     RLAGATFTIPPLRDRKDEVLPLAEQFVSSAAGPLGRAFALSEEARTWLGQHDWPGNIR
                     ELRNACERAVLLATGSVIEKSHLTIDEPKRPAQRFRSPTTPPPGTLTSDMPTQVRATV
                     AELEKQRILEALDKCAGNQTRAAELLGISRRTLINRLDEYGIARPRKRDE"
     gene            3738..4331
                     /locus_tag="VFV99_28510"
     CDS             3738..4331
                     /locus_tag="VFV99_28510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063365.1"
                     /translation="MSVELELTCPACLHHAGVLLVDESLYVKCPGCDVLLGLGPTKLL
                     MPLNGFEPPQRQLTRRVDKPKRTKVRLRRAGDALRAGIRFGIFGILTPFAKKAELSPA
                     GYTMRTWRGGGSELPLGDVVGVIVLQMFLGWLIGDKVENRAQLVWISTLVYRRSDDVM
                     YRPMYMHSREDAEYLASALNAHLAELKSAAPHPYRGA"
     gene            4358..4807
                     /locus_tag="VFV99_28515"
     CDS             4358..4807
                     /locus_tag="VFV99_28515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063366.1"
                     /translation="MRAITPAGALALVVALSQTLLVACAGDPDYVVQPGVIEFYDEQG
                     PIVVPSTASVGSTFTVEVTTFGGGCESFVNTAVAHVADGAIVTPYDRSHIPGENEACT
                     DILHTFHHEAALSFDTAGSKIVRFHGRRHRGNVDEDILLPYTIVVNE"
     gene            4843..6009
                     /locus_tag="VFV99_28520"
     CDS             4843..6009
                     /locus_tag="VFV99_28520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063367.1"
                     /translation="MKSVALPVGATQFVAGGGRDVDTAKLTLDFAAGEHVQITDQNGD
                     EIGIAIADPENARLRVMAMLADGFPKIDGALIGWRVERALRWRQQLGLVGPTNAYRVV
                     HGAGDGLPGFTADVLGSTAVVYAYGEGLKAVGKQVAEAVVGFAKLDGAVVKVRARGGA
                     SEVTQEIVGKAPAESSVVTEHDVPFEVHPLGGLNHGLFTDMREQRRGLYRFAAGKRVL
                     NLFSYTGALSVTCARAGAASVTSVDTSQGVQAWARGNFGRSGLNDDKKWKFETGDAVR
                     FLARAAKDKERYDLVIIDPPTFSTARGAPWTLDRDYPKLIAQAAAVIPTDGVLWLAAN
                     THELGSLAKLALKGLREAGKSGAIVEQGGLPPEYPTVAAQPHDRYLQICVLRLG"
     gene            complement(5995..6564)
                     /locus_tag="VFV99_28525"
     CDS             complement(5995..6564)
                     /locus_tag="VFV99_28525"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX5063368.1"
                     /translation="MNPIFEAALEIEAICRAANFKFCFIGGLAVQRWGQPRMTADVDL
                     TVVTGFGGEDRYVDRLLAKLSGRIPDAREFALRHRTLLVAASNGIHADIALGAMPFEE
                     RAAERASPFAISESASVTTCSAEDLIVHKAFAGRDKDWLDIEGIVIRQGDHLDPQLIW
                     AELLPLLELREDTGTTETRLRRLLELAKA"
     gene            complement(6866..8341)
                     /locus_tag="VFV99_28530"
     CDS             complement(6866..8341)
                     /locus_tag="VFV99_28530"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5063369.1"
                     /translation="MRWKSPVVAALLGAGSLAHAGTYHVATSGSDTNPGTAAAPFRTI
                     QKAADVVAAGDSVTIHAGTYTGFVVTKHGTQTAPITFSGSGTVNIDGASTTDRDAVHI
                     EGASYITVEGLTVTGATRAGISALDSDHITVRKNKVDSNAKWGVFSSFTDDFVVENNE
                     LSRSGTQHGVYASNSADHPVIRGNYIWGNAMCGVHMNGDINMGGDGVITGAIVENNII
                     KDNGRLGGSGINGDGVTNALIRNNLLDGNHASGISLYQIDGGAPSTGNQVINNTVRMA
                     SNARWALNIQDGSAGNTLRNNILLNPTAGRGAIDICAACLTGLVSDHNAFVNTLMIDG
                     ATMNLATWQTRTAGDAASFAATDADLFTSATDLTLRAGSKAIDKGMATGAPPTDLAGT
                     SRPQGTGVDIGAYEHCDGACVGGGSGSGSGDGSGSGDGSGSGDGSGSSTDGGGDGSDY
                     SGEGGVSDRNGGCNVVGGGAGACGSALVLAAAAMLVRRRRR"
     gene            8483..9247
                     /locus_tag="VFV99_28535"
     CDS             8483..9247
                     /locus_tag="VFV99_28535"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5063370.1"
                     /translation="MCGRYTITRQEGLIEEMEAVLDGKVEANEWWKPRFNVAPTQDAP
                     VVTLKDGVRTIEMMRWGLVPFWAAKPGAAKPPLMINARIESVESKRMFRDLLERKRCL
                     VPADGFYEWKKISSKQKQPMFIHPGDHRFFAFAGLWARAKLPTGELHSFTIITGPPND
                     LVKPIHDRMPVVLDRSVWSAWLDPSLDAEGARALLTVPPVADWQADPVSKWVNVADHD
                     DPQCVAPVAEETAEPDEKKPKAKKKKSSGGQGSLFD"
     gene            complement(9244..>9510)
                     /locus_tag="VFV99_28540"
     CDS             complement(9244..>9510)
                     /locus_tag="VFV99_28540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063371.1"
                     /translation="RVTLERVWSLHQAQHADLVVRALDDLGIPCHVSSANLRTVLAFF
                     GPFAPIDVYVAPEHMPAARTRIRELIEPASAAEPATASEPAKAS"
BASE COUNT         1651 a         3193 c         3142 g         1524 t
ORIGIN      
        1 gactcgcgcg ctggcgcagc gaggatgctg ccgatcgcgc gctactcgct gacgtcggcg
       61 ccgccgcccg ccgctggaac gagtccggcc ggcccgccga cctgctgtgg cgtggccaag
      121 cgctcgccga gctcacgcgg ctcacgaagc gctcgacggc actcaccgag ctcgagcgcg
      181 catttgcgac cgacgccgca agcgcgcagc aacgcgctcg tcgcatccgt cgcggcatcg
      241 tcgcgggcgt gatgatcgtg ctcgccgccg ctgcgagcgt gatggcgtac ctcagcgtcg
      301 tcgcgaacga gaaccgtgcc gaggccgagc gcaacgcaac cgccgcgaag caatccgccg
      361 cgctcgccga cgagcggctc accgctagcc tgatcgcgca gggccgccgc gagctcaacg
      421 acaatcgcgc gatgcccgcg ctcgcctact tcgcagcggc gatgaaacgc ggcgccgaca
      481 cggccggcct gcgcgcgatg gtgtcgatcg cgagccgcgg ctggaaagac acgctcgtca
      541 catcgcacgc cgcgccgttc tcgtcggtga ccggctcacc gaacggctgg atcgctgccg
      601 gcgatcaggt cggcaccgtg cactactggt cggacaccgg cgtgtcgctc ggtgaggtga
      661 aaaccgaggt cggcccgatc gcgaccgtgg agcgccagcc cgacgacacg ctggtcgtca
      721 tcggcaacac cggcgtcgtt ctgctcggca cgaagcacga ggtgctgcgg cgcgtgcaaa
      781 ccgagaagtc gtggtacgcg cgcttcggac caggtcccga cgagctgacc gcggtcggcg
      841 acggcaagct tcgcatctac ggattcgacg gcaaggtccg ccgcgagatc gcgctcgaca
      901 cgaccgccag cggtgtcgag ccggtgttcg caccgtcgat gtcgcacgca ctcgtcaacg
      961 tcggcgacga catcgcgaag ctcgatctcg tgacgatgag acaaacgatc atcgtcaaga
     1021 acggctgggg gcctcccggc ttgtcgcgca cgctctcgac ctacgcgtac gtggacaagg
     1081 accaccgcgt ccacgtcatc gatctcgacg gcaagccgat caagacgctc gacgtcaagt
     1141 cacgcacgta cggcatcgtg ttctcgcagg atggcgatcg catcggtgtc gtcggcgacg
     1201 cctacatgac gatcttggac gcgaagggcg agcgtctcgg cgaatatccc atcgcgcgcg
     1261 accaatcgct gttcctgttg cgcggcgacc acgcctggat caccggcaac cagggcatcg
     1321 tccgtcacta tagcgacggc gagctcgtgt cgtcgatccc tgcgttcgcg gtcgaggtcc
     1381 aagcatctgc ggtcgcgcgt gacgcagtcg cgctgatcgg cagcgacgcg agcctcgcga
     1441 tcatccgcgc gaattcagcg cagatcgtcg aggacaaaga agtctgcgac aagccagcgt
     1501 acgcatcgaa cggcatcgcg accggctacg agtgcgccgg gcacgtgaag ctctatctcg
     1561 gccgcagcta catcgcagag tacccggtga gcgagatgca gctcgcggtc gcctacgacg
     1621 aggcgtcgca tcgcagcgca gtcagcggcg gcgccggcgt gcacatcttc gatcgcgacg
     1681 cgaaggtgat cgcgaagacg gcgcgcgagg gccgcgctgc gttcgaggac ggcgatcacg
     1741 ttctcgtcgc cgaggccaag aaggcactgt ggcggtggac gatctcgacc gacacctggc
     1801 aagagctgat gccgatcggc gatatctacg cgctcgcggt cctgcccggc aagatcctgc
     1861 tcggccagca agacggcaag ctgctcgtga tcgagaacgg ccacgagacc caccgcgccg
     1921 atgtcggcac gcagatcggc gacatcgtcg cctcgcccga caagcgttgg ctcgccgtca
     1981 gcctcgcgac cggcgctgtc gcgatcgtcg atgcccagtc ctggcaggtc acccgcatgc
     2041 tcgccgcggc tgacaactac ggcatggcgg cgacgttcga tggcaccggt gacctgctca
     2101 tccgctccag ccgcaacgcg ctcacgatct gggaccgtgc aagcggcgag gagctgcttt
     2161 tcgggttcga tctcctccag gacctgagca acgggcgctt cctgcccgat ggccgcatcg
     2221 agatcgaccg ccggagcccc ggtctcctcg atattcctcg tgatacccgc cccattgcgc
     2281 agctcgtcag agacatcgac tgcaaggtcc cgctcaaagt tgtaggaagc cggatcgaac
     2341 cgcagatacc gagctgtcca tgagttgcaa ctcgggagca tccggccgta cgctagcctc
     2401 gtgatcgacg acgacgttgg cgcaactacc gaacaaatcg atcgtcaggc cccgttcggt
     2461 gatcgcgtgt tgctcgtcat cgacggcgcc tcgctgcagg tcgtacccct gcccggcacc
     2521 ggcacgctga cgatcggccg ctcccagaag tgcgacgtgg ccatcgactc cggctccgtc
     2581 tcgcgtcacc acgcgaacct cctggtcagc caggacgtca acatcgagga cgtcggcagc
     2641 tcgaacggca cgttcgtcga tggccagcgc ctcgacgcga acaagcacgt tcgtatcggc
     2701 atcggcatcc cgttcctcat cggcggcgtc accgtcatgg tgcaaacgcg cgccggcgcc
     2761 cggcccgccc ccgcttcgaa aaccagcaag ctcgcggccc tcgagcaatc ggccgctcgc
     2821 atcgcagtcg gcaagctcgc cgtcctcatc ctcggcgaga ccggtgtcgg caaagaacgc
     2881 ttcgccgagc gcctgcacga gatgtcaccg cgccggcccg caccgttcgt ccgcatcaac
     2941 tgcgccgcga tcagcgaacc cctcctcgaa gccgagctgt tcggcaacga aggcacgaac
     3001 aagcccggcc tgatcgagca cgccgacggc ggcaccgcgt tcctcaacga cgtgtcggag
     3061 ctgccgcagt cgctgcaagt gaagctgctg cgcgtcctcg aggacaacca ggtccgccgc
     3121 atcggctcgg cgaacccgcg cacggtcgac gtccgcttca tcgcggccac caacaaggac
     3181 atgcaggccg aggtcgacgc cggccgcttc cgccgcgacc tctacttccg cctcgcgggc
     3241 gccacgttca cgatcccgcc gctgcgcgat cgcaaagacg aggtcctgcc cctcgccgag
     3301 cagttcgtct cgtctgccgc cggcccgctc ggccgcgcat tcgcgctctc cgaagaagcg
     3361 cgcacgtggc tcggccagca cgactggccc ggcaacatcc gcgagctgcg caacgcatgc
     3421 gagcgcgccg tgctcctggc cacaggctcg gtgatcgaaa aatcgcatct cacgatcgac
     3481 gagccgaagc ggccggcgca gcggttccgc tcgccgacga cgccgccgcc cggcacgctc
     3541 acgagcgaca tgccgacgca ggtccgcgcg accgtcgccg agctcgagaa gcaacgcatc
     3601 ctcgaagcgc tcgacaagtg cgccggtaac cagacccgcg ccgccgagct gctcggcatc
     3661 tcgcgccgca cgctgatcaa ccgactcgac gagtacggta tcgcgcgccc gcgcaagcgc
     3721 gacgaatagc acgcgcagtg tcggtcgagc tcgagctgac gtgccccgcg tgcctgcacc
     3781 acgcgggcgt gctgctcgtc gacgaatcgc tatatgtcaa atgccctggc tgcgacgtcc
     3841 tgctcggcct cggcccgacg aagctgctga tgccgctgaa cggcttcgaa ccgccgcagc
     3901 gtcagctcac gcggcgggtc gacaagccga agcgcaccaa ggtccggctg cgccgtgccg
     3961 gtgacgcgct ccgtgccggg attcgcttcg ggatcttcgg gatcctgacg ccgttcgcga
     4021 agaaggccga gctctcgccc gctggctaca cgatgcggac gtggcgcggg ggcggctcgg
     4081 agctgccgct cggtgacgtt gtcggcgtga tcgtgttgca gatgtttctc ggctggctaa
     4141 tcggcgacaa ggtggagaac cgcgcgcagc tcgtgtggat ctcgacgctc gtgtatcgcc
     4201 gcagcgatga cgtcatgtac aggccgatgt acatgcactc gcgcgaagac gccgagtacc
     4261 ttgcctcggc gctcaacgcg cacctcgccg agctgaagag cgcggcgccg catccttatc
     4321 gcggtgcgtg aacgctttgg tcgtgtacgc tgtaggaatg agagcgatca ctccggccgg
     4381 tgcgttggcg ctcgtcgtgg cgctatcgca gacgcttctt gttgcctgcg caggcgatcc
     4441 cgactacgtc gtgcagccag gcgtgatcga attctatgac gagcaagggc ctatcgtcgt
     4501 accgagcaca gcgtcggtgg gcagtacgtt cacggtcgaa gtgacgacgt tcggtggtgg
     4561 ctgcgagtcg ttcgtgaata ctgcagtggc tcacgtcgcc gacggcgcca ttgtcacgcc
     4621 gtacgatcgc tcccacattc cgggcgagaa cgaggcatgc acggacattc tgcacacctt
     4681 ccaccacgag gcagcgctgt ctttcgatac cgcaggctcg aagatcgttc gcttccacgg
     4741 tcgacgccac agagggaacg tcgacgagga catcctgctt ccttacacga tcgtcgtgaa
     4801 cgaataggtc accggacgac gccactccga taagctcgcc cagtgaaatc cgtcgccctg
     4861 cccgtaggcg caacacagtt cgtggctggt ggtggacgtg atgtcgacac cgcaaagctg
     4921 acgctcgact tcgcggccgg cgagcacgtg cagatcaccg atcagaacgg cgacgagatc
     4981 gggatcgcga tcgccgatcc ggagaacgcg cgcctgcgcg tgatggccat gctcgccgac
     5041 ggctttccaa agatcgatgg tgcgctgatc ggctggcgcg tcgaacgcgc attgcgctgg
     5101 cggcagcagc tcggcctggt gggcccgacg aacgcgtatc gggtcgtgca tggcgcgggc
     5161 gatggcttgc cagggttcac ggccgacgtg ctgggctcga cggcggtcgt gtacgcgtat
     5221 ggcgagggcc tcaaggccgt cggcaagcag gtcgccgaag cggtcgttgg cttcgcgaag
     5281 ctcgacggcg cggtggtgaa agtccgcgcg cgcggcggtg cgagcgaagt cacgcaagag
     5341 atcgtcggca aagcgccagc cgaatcatcg gtcgtaaccg agcacgatgt gccgttcgag
     5401 gtacatccgc tcggcggcct caatcacggg ttgttcaccg acatgcgcga gcaacggcgc
     5461 gggctgtatc gcttcgcggc gggcaagcgc gtgctgaacc tgttcagcta caccggtgcg
     5521 ctgagcgtga cgtgcgcgcg tgctggcgcg gcgagcgtga cgagcgtcga cacgtcgcaa
     5581 ggcgtgcagg cgtgggcgcg cggcaacttc gggcgctcgg gcttgaacga cgacaagaag
     5641 tggaagttcg agaccggcga tgcggtgcgc ttcctggcgc gcgcggcgaa ggacaaggag
     5701 cgctacgacc tcgtgatcat cgatccgccg acgttctcga cggcgcgcgg cgcaccgtgg
     5761 acgctcgatc gcgattatcc gaagctgatc gcgcaggccg cagcggtgat tccgaccgat
     5821 ggcgtactgt ggctcgcagc gaacacgcac gagctcggct cgctcgcgaa gctcgcgctc
     5881 aagggattgc gcgaggccgg caagagcggc gcgattgtcg agcaaggcgg cttgccgccc
     5941 gagtacccga cggtagcggc tcagccgcac gatcggtatc tgcagatttg cgtcctacgc
     6001 cttggctagt tcgagcaggc gtcgcaggcg agtttcagtc gtccccgtgt cctcgcggag
     6061 ctcgagcagc ggcagtagct cggcccagat cagctgcgga tcgaggtggt cgccctggcg
     6121 aatgacgatc ccctcgatgt ccaaccagtc cttgtcgcga ccagcgaatg ccttgtgcac
     6181 gatcagatcc tcggcagagc acgtggtgac gctcgctgac tccgagatcg cgaagggcga
     6241 cgcacgttcg gccgcgcgct cctcgaacgg cattgccccg agtgcgatgt cggcatggat
     6301 tccattactc gcagcgacca gaagcgtgcg gtgacggagt gcgaactccc gagcatcagg
     6361 aatgcggccg gaaagcttcg cgagcagcct gtcgacgtac cgatcctcac caccgaaccc
     6421 ggtgacgacc gtgaggtcga cgtcggcggt catacggggc tgcccccacc gctggaccgc
     6481 taggccaccg atgaaacaga acttgaagtt cgcggcgcga cagatcgcct cgatctcgag
     6541 agccgcttcg aagatcgggt tcaccggcgc ccgcgcgcga acaagcgttg ttgctcgacc
     6601 aggcctgacg ttaaacggcg cgctggcgcg atcgcatcaa ccggcgtagc ggcgagcaag
     6661 gcgtcggcca agcgaagcgc ctcggcgggc tcgagccggc gaagttcgtc tgcttcgatc
     6721 gccgcgaccg cgtcggcgcc tgcatcgcgt tggcgaagcc aggcacgagc gaaggacgga
     6781 agcgtggaca tcgatcaaac cttagcagac tcggcggtac gatcggtatt ggccgatttg
     6841 cgtgggacgc gtaggccaac tggcgttagc gacgacgccg gcgcacgagc atggccgccg
     6901 cggcgagcac caacgcgctg ccgcacgcgc cagcgccccc accgacgacg ttgcaaccgc
     6961 cattgcggtc gctgacgccg ccttcgccgc tgtagtcgga gccatcgccg ccgccgtcag
     7021 tgctcgagcc gctgccatca ccgctgccgc tgccatcgcc gctgccgctg ccgtcgccgc
     7081 tgccactgcc actgccgcca ccgacacacg caccatcaca gtgctcatac gcgccgatat
     7141 caaccccggt gccctgcggg cgcgacgtgc cagcaagatc ggttggcggt gcgccggtcg
     7201 ccatgccttt gtcgatcgcc ttcgagccgg cgcgcagcgt cagatcggtc gcgctcgtga
     7261 acaggtccgc atcggtcgca gcaaacgacg ccgcatcgcc cgccgtacgc gtctgccacg
     7321 tcgcgaggtt catcgtcgcg ccgtcgatca tgagcgtatt gacgaacgcg ttgtgatcgg
     7381 agaccaggcc cgtgaggcac gcggcgcaga tatcgatcgc accgcgaccg gccgtcgggt
     7441 tgagcaggat gttgttgcgg agcgtgttgc ccgccgagcc gtcctggatg ttgagcgccc
     7501 agcgcgcatt gctcgccatg cgcacggtgt tattgatgac ctggttaccg gtcgatggag
     7561 cgccaccgtc gatctggtac agcgagatac ccgacgcgtg attgccgtcg agcaggttgt
     7621 tgcggatcag ggcgttggtg acaccgtccc cgttgatgcc ggagccaccg aggcgaccgt
     7681 tgtccttgat gatgttgttc tcgacgatcg caccggtgat gacgccgtca ccacccatgt
     7741 tgatgtcgcc gttcatgtgg acgccgcaca tcgcgttgcc ccagatgtaa ttgccgcgga
     7801 tgaccgggtg gtcggcgctg ttcgacgcgt agacgccgtg ctgcgtgccc gagcgcgaca
     7861 gctcgttgtt ctcaacgacg aagtcatcgg tgaacgacga gaacacgccc cacttcgcgt
     7921 tgctgtcgac cttgttcttg cgcaccgtga tgtgatcact gtcgagcgcg ctgatgcccg
     7981 cgcgcgtcgc accggtcacc gtgaggcctt cgacggtgat gtacgacgcg ccttcgatgt
     8041 gaacggcgtc gcggtccgtg gtcgatgcgc cgtcgatgtt gacggtgccg ctgccgctga
     8101 acgtgatcgg cgcggtctgg gttccgtgct tggtgacgac gaagccggtg tacgtgccgg
     8161 cgtggatcgt cacgctatcg cccgcggcaa cgacgtcggc cgccttctgg atcgtgcgaa
     8221 acggcgccgc cgccgtgcct gggttggtgt cgctgcctga ggtcgcgacg tgataggtgc
     8281 ccgcgtgggc gagagagcca gcgccgagaa gcgcggccac gacaggagac ttccacctca
     8341 tgagccgcgc accgtagcaa gcagttgccg cgcgtctctg tggttgttct cgcacggtat
     8401 cccatcgaag aaatgccgag ccgcatctac gcgagttacg cgacagcgca tcgaacaagg
     8461 gctggtagag tttctgagcg ccatgtgcgg ccgctacacc atcacgcgtc aggaaggcct
     8521 catcgaggag atggaggccg tgctcgacgg caaggtggaa gcgaacgagt ggtggaagcc
     8581 gcggtttaac gtcgcgccga cacaagacgc gccggtcgtg acgctgaaag acggcgtgcg
     8641 cacgatcgag atgatgcgct ggggcttggt gccgttctgg gccgcgaaac ccggcgccgc
     8701 gaagccgccg ctcatgatca acgcgcgtat cgagagcgtc gagagcaagc gcatgttccg
     8761 cgacctgctc gagcgcaagc gctgcctcgt gcccgcggat ggcttctacg agtggaagaa
     8821 gatcagcagc aagcagaagc agccgatgtt catccatcct ggcgatcatc ggttcttcgc
     8881 gttcgcggga ctgtgggcgc gcgcgaaact accgaccggc gagctgcaca gcttcacgat
     8941 catcaccggg ccgccgaacg acctcgtcaa accgatccac gatcgaatgc cggtcgttct
     9001 cgatcgcagc gtgtggagcg cgtggctcga tccgtcactc gacgccgaag gcgcacgggc
     9061 gctcctgacg gtgccgcccg tcgcggattg gcaggcggac ccggtgtcga agtgggtcaa
     9121 cgtcgccgac cacgacgatc cgcagtgcgt cgcgccggtt gccgaagaga cggccgagcc
     9181 cgatgagaag aaaccgaagg caaagaagaa gaagtcgtcg ggcggccagg gctcgctgtt
     9241 cgattagctc gccttcgcag gctcgctcgc ggtcgccggc tcggccgcgc tcgccggctc
     9301 gatgagctcg cggatccgcg tacgcgcggc gggcatgtgc tcgggtgcga cgtagacatc
     9361 gatcggcgcg aacggaccga agaacgcgag cacggtgcgc agattcgccg acgacacgtg
     9421 gcacgggatg ccgaggtcat cgagcgcgcg cacgacgaga tccgcgtgct gcgcctgatg
     9481 caacgaccac acgcgttcga gcgtgacgcg
//
LOCUS       DASUFX010000710         8536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1225161, whole genome shotgun sequence.
ACCESSION   DASUFX010000710 DASUFX010000000
VERSION     DASUFX010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225161"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFV99_28545"
     CDS             <1..357
                     /locus_tag="VFV99_28545"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX5063372.1"
                     /translation="DPRDIELLLAGIKLTREIAATSPLKEMLGAEIVPGPHAASDDDL
                     RAHIRATCNTIFHPTGTCKMGKDPMAVVDHELRVHGLRGLRVADASIMPRITGGNTNA
                     PTIMIAEKCAELALAQ"
     gene            complement(358..669)
                     /locus_tag="VFV99_28550"
     CDS             complement(358..669)
                     /locus_tag="VFV99_28550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063373.1"
                     /translation="MGKPDDRKSRTTREFDSARLAALTKEQLEEGDEELQQSVAPEAL
                     PAPLIGRAATIDDPMTTGLLAEVARRSQTADFDEELIKEVLSKIDSGDTNHPHTRKRS
                     R"
     gene            896..2041
                     /locus_tag="VFV99_28555"
     CDS             896..2041
                     /locus_tag="VFV99_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5063374.1"
                     /translation="MDRSLFTEEHETFRKAFRQFCEREIVPNQARWREQGQVDREVWR
                     KAGAQGFLCTWMEEKYGGAGADFLCATIIMEELARVYESGFAMGLHSDIIVPYIHTFG
                     NEEQKQKWLPGCASGEIVTALGMTEPGTGSDLAALATTAVKDGNDYVINGSKTFISNG
                     QLCDLVVVAAKTDPNPENAHRGISMFVVEANRKGFQKGKRLHKMGMASQDTSELFFED
                     CRVPATNMLGAEGAGFMMMMTKLQQERLAVAIGAVAGAEQVLADTITYTKERKAFGKP
                     ISKFQNTQFKLVECLAKVEVGRAYVDKVIAGHIKGEHLVKECSMAKFWTTDMAQEVID
                     TCLQFFGGYGYMLEYPVTRAFMDARVQRIYAGTNEIMKIIVAKQLGL"
     gene            2083..2397
                     /locus_tag="VFV99_28560"
     CDS             2083..2397
                     /locus_tag="VFV99_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5063375.1"
                     /translation="MKAACASACQPAAELDPAVDEELATLAKAFAHPVRVQIVRILAA
                     TEGCVNELVEQLPLAQSTVSQHLKVLKDAGLVRGTVEGPRTCYCIDPAGLRRLRILVA
                     GL"
     gene            2411..3073
                     /locus_tag="VFV99_28565"
     CDS             2411..3073
                     /locus_tag="VFV99_28565"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX5063376.1"
                     /translation="MARRYAAEALGTALLLEAVVGSGIMAEALSGGNVGVALLANAIA
                     TGGALYVLILGFGPISGAHFNPLVSLIEAAFKRLPRAQLPGYLLAQFAGAFAGVLLAH
                     AMFNLELVQHSHHVRSTHGEIISEVVAAFGLWLVIATSSRADPSKAPAAVALYIVAAY
                     WFTASTSFANPAVTVARAFTNTFAGIRIDDVAPFIGAQLVGGGLAAALTAWFFSRTTE
                     ST"
     gene            3135..4025
                     /locus_tag="VFV99_28570"
     CDS             3135..4025
                     /locus_tag="VFV99_28570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063377.1"
                     /translation="MSDKPGVLFLCVANSARSQLAEGLARARFGERLRIQSAGSQPTQ
                     VNPFAIETLAEVQLDITSHASKLVDSVDPDGVDLVITLCAEEVCPAFLRPVRRLHWPI
                     PDPARKTPLPDAEMRTRFRAARHAIAARLDGLEAALALPPRTMIMPASESDRDELSAL
                     LTAANLPLDGLDDAFPHGFALARIDGALVGVAGVEHHGDHDGLLRSVAVDAPYRGKHV
                     GEALVADRLAWAKSQLGCAAAPRFATISLLTTEADQYFERLGFQRIARDELPAALARS
                     TQLQISRCSSAVAMIRRVSS"
     gene            4078..4974
                     /gene="arsM"
                     /locus_tag="VFV99_28575"
     CDS             4078..4974
                     /gene="arsM"
                     /locus_tag="VFV99_28575"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX5063378.1"
                     /translation="MKTDDQIRSDVREHYGAIARSAQACCEPTAKTPQAGCCAPTATG
                     FERGEASRVLGYSAADMRAVPETANLGLGCGNPQAIAELRAGEVVVDLGAGGGFDAFL
                     AARAVGQTGRVIGVDMSADMVALARTNAAKHAAANVEFRLGEIEHMPVPDATADVIMS
                     NCVINLAPDKRAVYAEALRVLKPGGRLAISDVVALAPMPQHLADDVAARCGCIAGAAL
                     ASELPQLLAEVGFTDVRVDIDPASADVIASWAPGEGLERLVASARIRASKPGGPANKS
                     AGPAGADTGTKAGACCEPGCCS"
     gene            4947..5534
                     /locus_tag="VFV99_28580"
     CDS             4947..5534
                     /locus_tag="VFV99_28580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5063379.1"
                     /translation="MLRAGVLLVIATSAQAVAWDALRGELYRFVARRVPGPDADDIVQ
                     ESLLRIHRGLATVREDGALVGWMYQVTRNALADHLRASRPTDELEDEDLVEPIDPDDS
                     AFASLTRCVAPFVGMLPAHYREAVELVELRGVSQVQAAAQLGIPVSTMKSRVQRGRAQ
                     LRELLDACCAIDLDARGHVVEVTPRCRCAAAPATT"
     gene            5594..6181
                     /locus_tag="VFV99_28585"
     CDS             5594..6181
                     /locus_tag="VFV99_28585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063380.1"
                     /translation="MRMVIGVLALVAMSPLAAAQPPGLTDTLPAPVDHGRDTSRVTFG
                     AGIGDFASYPMSIDDPGLALFVSKPLWLGNRYRFFQWVAEANALVGLGYTTMHGYAAV
                     GPQFGWNFYFGSVFGLEFRFGVDGLLQAGTRTVAGLGMGGSGAYVFRFWDDDRKRVKL
                     MMQMHAGGYFADDPGNDLGANAGAFALGLAYEKPI"
     gene            complement(6230..6484)
                     /locus_tag="VFV99_28590"
     CDS             complement(6230..6484)
                     /locus_tag="VFV99_28590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063381.1"
                     /translation="MVYITHVRFDGEGTGPEHIIMLRWRQAMGDTNVDDMIRWIESGG
                     VAKVSVLHAGGSREEQVKVVRDPGKRPYLFAPSFAELPRF"
     gene            6597..7493
                     /locus_tag="VFV99_28595"
     CDS             6597..7493
                     /locus_tag="VFV99_28595"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063382.1"
                     /translation="MKTAVIDIGTNTLLLLIVDEKLQRVVDLCRFGRLGKGLDASGRL
                     ANEAIAKSLDICREYRSVMTEHGITKPHIIGTQALREAANARDFVVPAEDILGGSIEI
                     IVGPREAELAYLSVARTFPELGDSCFVVVDVGGGSTELITANDGGVVNAVSVPIGAVR
                     LTERYLLSDPPAVGEGLRLDDKIDRTLAPLGLPSGVPVIGTAGTATTMAAASLGLTTY
                     DPDKVTGHKMSADEVLQLRQRLFAATTAQRREMPGVEPQRADVIAAGVAIYHRVMHRI
                     GAPTLITCDRGIRWGLAYELAK"
     gene            7539..>8536
                     /locus_tag="VFV99_28600"
     CDS             7539..>8536
                     /locus_tag="VFV99_28600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5063383.1"
                     /translation="MRRILLAVGALVVIAVGLIWWLHGRGDHAPSKSKPTQVAGKHST
                     APRPPAKPATVSGRVTRKSDGAGVAGAVVSLAGAEFGAEFSTVKKPTLVAITDDKGAW
                     TAKDVPPGDYIIAATAKGLLPSARDKLTVASGEQRTGIDFALEAGGVAVRGTVSDVLG
                     GPIASARVTARRQELSLNRDAEFATLTGADGTYELTLPAAEYRMAAAHDDYTRATKSI
                     ELEDKPVTADFTLIPGGVVRGQVIARDTGKPVPHAIVTVEPAHRSLQSNEVSAVSDDE
                     GNFVVRGLSSGGVKIEARARGYSSTQPTTVQVGIGEDVDGVQVLVDGAFSISGRVV"
BASE COUNT         1390 a         2791 c         2900 g         1455 t
ORIGIN      
        1 gatccgcgcg atatcgagct gctgcttgct ggcatcaagc tgacgcgcga gatcgcggcg
       61 acgtcaccac tcaaggagat gctcggcgcc gagatagttc ccgggccgca cgccgcgagc
      121 gacgacgatc tgcgcgcgca catccgcgcg acgtgcaaca cgatctttca cccgaccggc
      181 acctgcaaga tgggcaagga cccgatggcg gtcgtcgatc acgagctacg cgtgcacggc
      241 ctgcgtggcc tacgcgttgc cgacgcgtcg atcatgccgc gtatcacggg cggtaatacg
      301 aacgcaccga cgatcatgat cgccgagaag tgcgcggagc tcgcgctcgc tcaatagtca
      361 gcgcgagcgc ttgcgggtgt gggggtggtt tgtgtcgccg ctgtcgatct tgctcaagac
      421 ttctttgatc agctcttcgt cgaagtccgc ggtctgcgag cggcgggcga cctcggccag
      481 aaggccggtc gtcatcgggt cgtcgatggt ggcggcgcgg ccaatgagcg gggccgggag
      541 agcctcgggt gccacggatt gctgcagctc ttcatctccc tcttcgagtt gctccttcgt
      601 tagggcggct aaccgagccg agtcgaattc tcgtgtagta cgagacttcc tgtcgtcagg
      661 cttacccatc tcgctaaagg taatgcacgc cgacatgtag agggaatctc aaaatgcgat
      721 ctgactgtgc gaccaaagag cacatcgaga ctcgcgcgat ctcgctcgcg ctccggatcc
      781 ataaccggat gcggtccggt tcaccgcgct gcgcttccta gccgccgaaa ggctctccta
      841 catcccgctc gcccgggcgc ggcgggacat ctcgcgtctg tgttacgaac ggcgaatgga
      901 ccgcagtctg ttcaccgaag agcacgagac cttccgcaaa gcgtttcgcc agttctgcga
      961 gcgcgagatc gttccgaacc aagcgcgctg gcgcgagcaa ggccaggtcg atcgcgaggt
     1021 ctggcgcaag gccggcgcgc agggcttcct ctgcacgtgg atggaagaga agtacggcgg
     1081 cgcgggcgcc gacttcctct gcgcgacgat catcatggag gagctcgcgc gcgtctacga
     1141 gagcggcttc gcgatgggcc tgcactccga catcatcgtt ccgtacatcc atacattcgg
     1201 taacgaggag cagaagcaga agtggctgcc cggctgcgcg tcgggcgaaa tcgtcaccgc
     1261 gctcggcatg accgagccgg gcactggttc cgacctcgcc gcgctcgcca cgaccgcggt
     1321 caaggacggc aacgactacg tcatcaacgg cagcaagacg ttcatctcga acggtcagct
     1381 ctgcgatctc gtcgttgtcg ccgcgaagac cgacccgaat cccgagaacg cgcatcgcgg
     1441 catctcgatg ttcgtcgtcg aagcgaatcg caagggcttc cagaagggca agcgcctcca
     1501 caagatgggc atggcgtcgc aggacacgag cgagctgttc ttcgaggact gccgcgtgcc
     1561 ggcgacaaac atgctcggcg ccgagggtgc cggcttcatg atgatgatga cgaagctgca
     1621 gcaggagcgc ctcgccgtcg cgattggtgc ggttgccggt gccgagcaag tgctcgccga
     1681 cacgatcacg tacacgaaag agcgcaaggc gttcggcaag ccgatcagca agttccagaa
     1741 cacgcagttc aagctcgtcg agtgcctcgc gaaggtcgag gtcggccgcg cgtacgtcga
     1801 caaggtcatc gccggccaca tcaaaggcga gcacctcgtg aaggagtgct cgatggcgaa
     1861 gttctggacc accgacatgg cccaggaagt catcgacacc tgcctgcagt tcttcggcgg
     1921 ctacggctac atgctggagt acccggtcac ccgcgcattc atggacgcgc gcgtgcagcg
     1981 catctacgcc ggcacgaacg agatcatgaa gatcatcgtc gcgaagcagc tcggcctgta
     2041 gcgcttgccg ccagcgccgc gggctgctat cgtaaagtta cgatgaaggc cgcatgcgcg
     2101 tcagcctgtc agcctgctgc cgagctcgat ccggcggtcg acgaggagct cgcgaccctc
     2161 gccaaggcgt tcgcacaccc ggtgcgcgtg cagatcgtcc gcattctcgc ggcgaccgag
     2221 ggttgcgtca acgagctcgt cgagcaactg ccgctcgcac agtcgacagt gtcgcagcat
     2281 ctcaaggtcc tcaaagacgc cggcctcgtt cgcggcacgg tcgagggacc gcgcacgtgc
     2341 tactgcatcg accctgcggg cctgcgccgg ctgcgcatct tggtggcggg gttgtaatcg
     2401 tgaaatgacg atggctcgcc gatacgctgc ggaagccttg ggcaccgcgc tgttgctcga
     2461 ggcggtcgtc ggatccggaa tcatggcgga ggcgctgagc ggcgggaacg tcggcgtcgc
     2521 gttgctcgcg aacgcgatcg cgaccggcgg cgcactctac gtgctgatcc tcgggttcgg
     2581 accgatctcc ggcgcgcact tcaatccgct cgtgtcgctg atcgaggccg cgttcaagcg
     2641 gctaccgcgc gcgcagctgc ccggctatct cctcgcgcaa ttcgcgggcg cattcgccgg
     2701 cgtgttgctg gcacacgcga tgttcaatct cgagctcgtg cagcactcgc accacgtgcg
     2761 atcgacgcac ggtgagatca tcagcgaggt cgttgctgcg ttcgggctct ggctcgtgat
     2821 cgcgacgtcg agccgggcag acccgagcaa ggcgccggcg gcagtcgcgc tatacatcgt
     2881 cgccgcctac tggttcacgg cgtcgacatc gttcgcgaat cccgcggtca ccgtcgcgcg
     2941 tgcgttcacc aacacgtttg ccgggatccg gatcgatgac gtcgcgccat tcatcggcgc
     3001 ccagctcgtc ggcggtgggc tcgcggccgc gcttaccgcg tggttttttt cgcgcacgac
     3061 ggagtcgacg tgaacgcgtt gctccgcaca gcgggcgatc tgcgatctga ctgcatgtcg
     3121 tcaggagccg gggcgtgagc gacaagccgg gcgtgttgtt tctgtgcgtc gcgaactcgg
     3181 cgcggagcca gctcgcggaa gggctcgcgc gggcgcgctt cggcgagcgg ttgcgaatcc
     3241 agagcgcggg ctcgcaacca acgcaggtca atccgttcgc gatcgagacc ttggccgagg
     3301 tgcagctcga catcacgtcg cacgcgagca agctcgtcga ttcggtcgat ccagacggcg
     3361 tcgatctcgt catcacgctg tgcgccgagg aagtctgccc cgcattctta cgacccgtgc
     3421 gccgactgca ctggccgatt ccggatccag cgagaaagac gccgctgccc gacgcggaga
     3481 tgcgcacgcg gtttcgcgcc gcgcgtcacg cgattgccgc gcgacttgac gggctcgagg
     3541 ccgcgctcgc cttgccgccg cgcacgatga tcatgccggc gagcgagagc gatcgtgacg
     3601 agctctctgc cttactcacg gcagcgaacc tgccgctcga tgggctcgac gacgcgttcc
     3661 cgcacggctt cgcgctcgcg cggatcgacg gcgcgctcgt cggtgttgcc ggcgtcgaac
     3721 atcacggcga tcacgacggc ctattgcgct cggtcgccgt cgacgccccg tatcgtggca
     3781 agcatgtcgg cgaggcgctc gtggccgaca ggctcgcgtg ggcgaagtct cagctcggct
     3841 gtgctgccgc cccgcggttt gcgacgatct cgctacttac gaccgaggcc gatcagtact
     3901 tcgaacggct cgggtttcaa cgcatcgcgc gcgacgagct accagccgcg ctcgcgaggt
     3961 caacgcaact gcagatctcg cgctgctcgt ccgcggtcgc gatgatccgg cgggtgtcgt
     4021 catgacgatt tcctgcgtcc atccggggcg acgcccgtct tcctggtcgg aggcaacatg
     4081 aagaccgacg atcagattcg ttccgacgtt cgcgagcact acggcgccat cgcacgtagc
     4141 gcgcaggctt gctgcgagcc gaccgcgaag acaccccagg ctggctgctg cgcgccgact
     4201 gcgaccggct tcgagcgcgg cgaggcgtcg cgcgtgctcg gctattccgc ggctgatatg
     4261 cgcgcggtgc cagagactgc aaaccttggt ctcggctgcg gcaatccgca agcgatcgcc
     4321 gagctgcgtg ccggcgaggt cgtcgtcgat ctcggcgccg gcggcggctt cgatgcattc
     4381 ctcgccgcgc gtgcggtcgg gcagaccggc cgcgtgatcg gcgtggatat gagcgccgac
     4441 atggtcgcac tcgctcgcac gaatgccgcg aagcacgcgg cagccaacgt cgagtttcgg
     4501 ctcggcgaga tcgagcacat gccggtgccc gacgcgaccg cagacgtcat catgtcgaac
     4561 tgcgtgatca acctcgcgcc ggacaagcgc gcggtctacg ccgaggcgct gcgcgtgctc
     4621 aagccaggcg gccggctcgc gatcagcgac gtcgtcgcgc tcgccccgat gccgcagcat
     4681 ctcgccgacg atgtcgccgc gcgctgtggg tgcattgccg gcgccgcgct ggccagcgag
     4741 ctgccccagc tgctcgccga ggttggcttc accgacgttc gcgtcgacat cgatccggcg
     4801 agcgcggacg tcatcgcgtc gtgggcaccg ggcgagggtc tcgagaggct cgtcgcatcg
     4861 gcacggattc gcgcgagcaa gccaggcggg cccgcgaaca agtcggccgg tcccgcgggc
     4921 gcggataccg gaaccaaggc cggcgcttgt tgcgagccgg ggtgctgctc gtgatcgcga
     4981 cgtcggcgca ggcggttgca tgggacgcgc tccgcggcga gctgtatcgg ttcgtcgcgc
     5041 gacgcgtgcc tggcccggac gccgacgaca tcgtgcagga atcgcttctg cgaatccacc
     5101 gcgggctggc tacggtgcgc gaggacggcg cgctcgtcgg ctggatgtat caggtcacgc
     5161 gcaatgcgct cgccgatcat ttgcgcgcgt cgcggccgac cgacgagctc gaggacgagg
     5221 atctcgtcga gccgatcgat cccgatgaca gcgcgttcgc gtcgctcact cggtgcgtgg
     5281 caccgttcgt cggcatgctg ccggcgcact atcgcgaggc ggtcgagctc gtggagctgc
     5341 gcggcgtctc gcaggtacag gcagccgccc aactcggcat tccggtctcg acgatgaagt
     5401 cgcgcgttca gcggggcagg gcccagctgc gcgagctgct cgacgcatgc tgcgcgatcg
     5461 atctcgacgc gcgtggccac gtggtcgagg tcacaccgcg atgccggtgc gccgcagcgc
     5521 ctgccacgac ctgatcgcga atagtttctg acttcgcgga tcggcgtcgg cgcagccgtg
     5581 gtatcggtcg cctatgcgga tggtgatcgg tgtgctggcg ctcgtggcga tgtcgccgct
     5641 cgcagctgcg caaccgccgg ggctgacgga cacgttgccc gcgccggtgg atcacggacg
     5701 cgatacgagc cgcgtgacgt ttggcgctgg catcggcgac ttcgcgagct atccgatgtc
     5761 gatcgatgat ccagggctcg cgctcttcgt gagcaaaccg ctctggctcg gcaatcgcta
     5821 tcggttcttc cagtgggtgg ccgaggcgaa cgcactcgtc gggctcggct acaccacgat
     5881 gcacggctac gcggccgtcg gtccgcagtt cggctggaac ttctacttcg gctccgtgtt
     5941 cgggctcgag tttcggttcg gcgtcgacgg cctgttgcag gccggcacgc gcacggtcgc
     6001 cgggctcggc atgggcggca gcggcgccta cgtgttccgg ttctgggacg acgaccgcaa
     6061 gcgcgtgaag ctgatgatgc agatgcacgc cggcggctac ttcgccgacg atcctggcaa
     6121 tgacctcggc gcgaatgcgg gagcgttcgc gctcggcctg gcgtacgaaa agccgatcta
     6181 gaccgtcgcg ctgcgtgagc ggcggtacgg gtgttcagct ggccggacct tagaagcggg
     6241 gcagctcggc gaagctaggt gcgaacaggt acggacgttt gcccggatca cgcaccacct
     6301 tcacctgttc ctcgcggcta cccccggcgt gcagaacaga taccttggcg acgccgccgc
     6361 tctcgatcca cctgatcatg tcgtcgacat tggtatcgcc catcgcctgc cgccaacgca
     6421 gcatgatgat gtgctcgggt ccggtgcctt cgccatcgaa gcgcacgtgg gtgatgtaga
     6481 ccatggcggg atgctctcag cagggactga catcgaagaa acgcgtgagc tagctcgctt
     6541 catccaggcg caggtcggcc gtcgcagagg gcgatcttcg cggtacgatt aggggcgtga
     6601 agaccgcggt catcgacatc ggtaccaaca cgctcttgct tctcatcgtc gacgagaagc
     6661 tgcagcgggt ggtcgacctc tgtcgcttcg gtcggctcgg taagggcctc gacgctagcg
     6721 gccggctcgc caacgaggcg atcgccaaga gtctcgacat ctgccgcgaa taccgctcgg
     6781 tgatgaccga gcacggcatc acgaagccgc acatcatcgg cacgcaggcg ctgcgcgaag
     6841 cggcgaacgc gcgtgacttc gttgtcccgg ccgaggacat cctcggcggg tcgatcgaga
     6901 tcatcgtcgg cccgcgcgaa gccgagctcg cgtacctctc ggtcgcgcgc acgtttcccg
     6961 agctcggcga ttcgtgcttc gtggttgtcg atgtcggcgg cggctcgacc gagctgatca
     7021 cggccaacga tggcggcgtc gtgaacgcgg tcagcgttcc gatcggcgcg gtgcggctga
     7081 ccgagcgtta tctgctgtcc gatccgcctg cggtcggtga ggggctgcgg ctcgacgaca
     7141 agatcgatcg cacgctcgcg ccactcgggt tgccctcggg cgtgccggtg atcggtaccg
     7201 cgggaaccgc gacgacgatg gcggccgcct cgctcggcct cacgacctac gatccggaca
     7261 aggtcaccgg ccacaagatg tcggccgacg aggtcctcca gctgcgccag cgcctgtttg
     7321 cagcaacgac ggcgcaacgc cgcgagatgc cgggcgtcga gccgcagcgt gcggacgtga
     7381 tcgcggcagg cgtcgcgatc tatcaccgcg tgatgcatcg gatcggcgcg ccgacgctga
     7441 tcacttgcga ccgtggcatt cgctggggcc tcgcctacga gctcgctaaa taacaattgt
     7501 gcaaccctgt gcaccgcgct cgggtctagg atgcgcgcgt gcgtcggatt ctgcttgcag
     7561 ttggtgccct cgtcgtcatc gcggtcggtc tgatctggtg gttacacgga cgcggcgatc
     7621 atgcgccgtc gaaatcgaaa cccacgcagg tcgctggtaa gcacagcacc gcgccgcgcc
     7681 ctcctgcgaa accggcgacg gtatcgggcc gggtgacgcg caaatcggac ggagcgggcg
     7741 ttgccggcgc ggtcgtctcg ctcgccggag cggagttcgg tgcggagttc tcgacggtga
     7801 agaagccgac gctcgtcgcg atcaccgacg acaaaggtgc gtggaccgcg aaagacgtgc
     7861 cgccgggcga ttacatcatc gcggcgaccg cgaagggtct gttgccgtcg gcgcgcgaca
     7921 agctgaccgt cgcctcgggc gagcaacgca cggggatcga cttcgcgctc gaggcaggcg
     7981 gcgtcgcggt gcgtggcacg gtgagtgacg tgctcggcgg gccgatcgcg agcgcacgcg
     8041 tgaccgcacg gcgacaggag ctctcgctca accgcgatgc cgaattcgcg acgctcaccg
     8101 gcgccgatgg cacgtacgag ctcacgctgc ctgcggccga gtaccgcatg gccgccgcac
     8161 acgacgacta cacgcgcgcc acgaagagca tcgagctcga agacaagccg gtgaccgcgg
     8221 acttcacgct gattccaggt ggcgtcgttc gcggccaggt gatcgcgcgc gataccggta
     8281 agccggtgcc gcatgcaatc gtcaccgtcg aacccgcgca ccgcagcctg cagtcgaacg
     8341 aggtaagcgc ggtatccgac gacgagggca acttcgtcgt gcgtggcctg tcgtcgggcg
     8401 gcgtgaagat cgaggcacgc gcgcggggct actcgtcgac gcagccaacg acggtccagg
     8461 tcgggatcgg cgaggacgtc gatggcgtgc aggttctcgt cgatggtgcg ttctcgatca
     8521 gcggccgcgt cgtgaa
//
LOCUS       DASUFX010000711         6683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_808559,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000711 DASUFX010000000
VERSION     DASUFX010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808559"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2599
                     /locus_tag="VFV99_28605"
     CDS             <1..2599
                     /locus_tag="VFV99_28605"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5063384.1"
                     /translation="QLAFAPDRHLVTDLAAFTSARRVELPTLEVHGETDAEKLRVDVD
                     PLRVLRQELDHARTFGDTEHVTPLLTALKKWGALGMRVAIASDSQSRTDRLLGVLTAR
                     DVAVRTAAAGEQPMAQPGVTLVPGTPAHSFASTKDGVALVTTSDIFGERVHHPSTKKK
                     RAKDALLGGVSDWSQLAPGDYLVHQKHGVGRYHGLKKIAVGTVSILQTTAVGAPTPKA
                     LEIDALHLEYDGGTLYLPVYRLGEVQRYVGAEGHAPKIDKLGGVTWEATQSKVKRHVR
                     ALAEELLQIYAQRASLPGHRFPPADESFRELEATFPFDETPDQQEAIDAVLGDMEAAR
                     AMDRLVCGDVGYGKTEVAMRAIFRAVQGGKQACVLAPTTVLVEQHFRTMTERFKGFGL
                     EIGRLSRFQARGEQVETVKKLTDGKLDVVVGTHRLLSPDVRFKDLGLLVIDEEQRFGV
                     AHKERIKKTKTQVDVLTLTATPIPRTLHLAMAGMRDLSIIATPPADRRAVRTFVSRVD
                     DATIKEAIERELARGGQIFWITPTIGQMGVREVDHRLPNDARPQRRTKPRDDDRTIED
                     WAEYIRGLVPAARVGIGHGSLSADALEKVMVQFVQGELDVLVATTIVESGLDIPRANT
                     MFVSRADAFGLAQLYQLRGRIGRSKERAYCYLLVPEPEHITDEARRRLEALQRFTELG
                     AGFQIASQDLEIRGGGELLGAKQSGSIAAVGFDQYVKMLESAVAELRGEPIHSEVDPE
                     LTVEMPGFIPDDYVPDPGQRLELYKRLSAVVDDDELKSVMTEIADRYGPVPGDAILLG
                     ELMGIKAMARKLGVLALEVSSTRVAIALPDDSPLHAKIPSSWRRLPDRRYAANPPARA
                     ALLVLLQRA"
     gene            2609..2896
                     /locus_tag="VFV99_28610"
     CDS             2609..2896
                     /locus_tag="VFV99_28610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063385.1"
                     /translation="MGRPKLDTYWFVFALASAALASVGLYKLATQREPQLGSPDYYSA
                     HVDQYDPYMWLFFAVLAGIMAAQLWAKKVSRIKVPDDIRRAEKRKRKQLQQ"
     gene            3063..4607
                     /locus_tag="VFV99_28615"
     CDS             3063..4607
                     /locus_tag="VFV99_28615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063386.1"
                     /translation="MKAASLVLAVLLVGCGHHSLNADSGLGSDDSMSGDDANGSDDGN
                     TGSDGGPGSDGGPGSDGGPGSDGGNTDGNTGGDGGNNNDGGGSGDGNTGPGACGLTVC
                     AKAFSSGTDGAALAVAVDSANNVYIAGLAESDLVFGTHTIFQLGARDAFVVSFAADGT
                     YRWAKRWGNGTKLAGAYGIAVDASNNIYIVGGMTGAVDFGGGLVGTQGMFVASFDTNG
                     NYRWARGDGGGSGLLAVAVADGKVFGIGRFLNSVTFSPTTTLTSAGAEDVVVAAFDTA
                     TGGFAWARSVGTAATDIEGGIVARTGVLAFTMSSGVIGTGERLVSWTTAGADRWSTPL
                     PAALDLRGLSVDAAGTLFVSGLHTGGIDVVPNAPYVNGDNEYVHAYTDGGSTGTSAWS
                     WTLSESPAHTVTAIPLTPSALDPAHDLVLAGTYEGTLYTGSTTLTSGNRTIFLAKYST
                     TGTPLAARRLDDASTAANEYVHGVAIATTGTTYVVGVYYGAPNFGDGALPFASQGGVM
                     ILRVAP"
     gene            4656..5732
                     /locus_tag="VFV99_28620"
     CDS             4656..5732
                     /locus_tag="VFV99_28620"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5063387.1"
                     /translation="MQKSGYFLVFAAALALGACHKKSDEDKAAREARAQGGPSAPPQS
                     AEELKTPLAKIDDVTITLGEFQERINRQSPYIRARYTSLEQKKEFLESLVRFEVLAKE
                     AYRRGLDKDPEVVRTMKQVMIQKLMRDEFDAKVTANDIPDADMKAYYDANLTEYVKPE
                     EVRVSAIILKNKAQAERVFLEAKGEAGKTNKGFRDLVMKYSSDEETKLRGGDLRYLEM
                     QSKDAPPAVIKAAFALVNTGDVSPVVDAGNGTFYVLKQTGRRKSITKSFDDAKPQIRN
                     KLFREKRLQEQKNFIDNLRAKAKIEINEPNLAKVRIDTSQKTGGDDGHGHDLAAPPPG
                     PPPGHDLQPAPPAQVPAGAGQPVP"
     gene            5751..>6683
                     /locus_tag="VFV99_28625"
     CDS             5751..>6683
                     /locus_tag="VFV99_28625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5063388.1"
                     /translation="MRKLLVLIVTLAIPAVAAAQKPASPNAGKKVGLERVVAVVNDSI
                     ILQSELEARLVPVRAEAEQIQDPTERRRRLAKLATQVLDEMVNDELIVQAAEAAKIEV
                     DTSEVQAALDEIKQQNNLDDAGLAQALASQGYTVQSYRQDLRRQLLRLRAVNQLVAPK
                     VQVTEDDVRSRYAELQRRSNAVSAVYLNHILIKLPEHPTEQQIAAAKDKAAQAIQMVN
                     SGTPFAEVAKQMSDDPNVELGWFQRGSINADWEQVVFSMQKGETRGPVTGPQGLHVFN
                     VSDVQSAALKPYPEMKDQLQRELRRRELDKQTAAW"
BASE COUNT         1220 a         2267 c         2194 g         1002 t
ORIGIN      
        1 gcagctcgcg ttcgcgcccg accgtcacct cgtcaccgat ctcgccgcct tcacgtcagc
       61 tcgccgcgtc gagctgccga cgctcgaagt ccacggtgag accgacgccg aaaagctgcg
      121 cgtcgacgtc gatccgctgc gtgtgttgcg ccaggagctc gatcacgcgc gcacgttcgg
      181 cgataccgag cacgtcacgc cgctgctcac cgcgctgaag aagtggggcg cgctcggcat
      241 gcgcgtcgcg atcgcgagcg attcgcagag ccgcaccgat cgcttgctcg gtgtgctgac
      301 cgcgcgcgac gtcgccgtgc gcaccgcggc ggccggtgag cagccgatgg cgcaacccgg
      361 cgtgacgctc gtccccggca cgcccgcgca ctcgtttgcg tcgacgaagg acggcgtcgc
      421 gctcgtcacg acgagcgaca tcttcggcga gcgcgttcac cacccgtcga cgaaaaagaa
      481 gcgcgcgaag gatgcgctgc tcggcggcgt ctcggactgg tcgcagctcg cgcccggcga
      541 ttacctcgtg caccaaaagc acggcgtcgg ccgctatcac gggctcaaga agatcgcggt
      601 cggcaccgtg tcgatcctgc aaacgacggc ggtcggcgca ccgacgccca aagctctcga
      661 gatcgacgca ctccacctcg aatacgacgg tggcacgctg tacctaccgg tctaccgact
      721 cggcgaagtg cagcgctacg tcggtgcgga aggccatgcg ccgaagatcg acaagctcgg
      781 tggcgtcacc tgggaggcga cgcagagcaa ggtcaagcgt cacgtccgcg cgctcgccga
      841 ggagctgctc cagatctacg cgcaacgcgc atcgctgccg ggccatcgct tcccgccggc
      901 cgacgaatcg ttccgcgagc tcgaagcgac gttcccgttc gacgagacgc ccgatcagca
      961 agaagcgatc gacgccgtgc tcggcgacat ggaagcggcg cgtgcgatgg atcgcctggt
     1021 ctgcggcgac gtcggctacg gcaagaccga ggtcgcgatg cgcgcgatct tccgcgccgt
     1081 gcaaggcggc aagcaggcgt gcgtgctcgc gccgacgacg gtccttgtcg agcagcactt
     1141 ccgcacgatg accgagcgct tcaaaggctt cgggctcgag atcggccggc tgtcgcgctt
     1201 ccaggcacgc ggcgaacagg tcgagaccgt caagaagctc accgacggca agctcgacgt
     1261 cgtcgtcggc acgcaccgcc tgctctcgcc cgacgttcgc ttcaaggatc tcgggttgct
     1321 cgtgatcgac gaggagcaac gattcggcgt cgcgcacaag gaacgcatca agaagacgaa
     1381 gacgcaggtc gacgtcctca cgctgaccgc aacaccgatc ccccgaacgc tccatctcgc
     1441 gatggccggc atgcgcgacc tctcgatcat cgcgacgccg ccggccgatc gccgggccgt
     1501 gcgcacgttc gtctcgcgcg tcgacgacgc gacgatcaag gaagcgatcg agcgcgagct
     1561 cgcgcgcggc ggtcagatct tctggatcac accgacgatc ggtcagatgg gcgtccgcga
     1621 ggtcgaccac cggctgccga acgacgcccg cccgcagcgc cgcacgaaac cacgcgacga
     1681 cgaccgcacg atcgaggact gggccgagta catccgcggc ctggtgccgg ccgcacgcgt
     1741 cggcatcggt cacggctcgc tctcggcgga cgcactcgaa aaagtcatgg tgcagttcgt
     1801 ccagggcgag ctcgacgtgc tcgtcgcgac gacgatcgtc gagagtggcc tcgacatccc
     1861 gcgtgcgaac acgatgttcg tctcgcgcgc cgacgcattc ggcctcgcgc agctctatca
     1921 gctccgtggc cgcatcggcc gctccaaaga gcgtgcgtac tgctacctgc tcgtgcccga
     1981 gcccgagcac atcaccgacg aagcgcgccg ccggctcgaa gcgctgcagc ggttcaccga
     2041 gctcggcgct ggcttccaga tcgcgtcgca ggatctcgag atccgcggtg gcggcgagct
     2101 gctcggtgcg aagcaatcgg gctcgatcgc cgcggtcggc ttcgaccagt acgtgaagat
     2161 gctcgagtcc gccgtcgccg agctgcgcgg tgagccgatc cacagtgagg tcgatcccga
     2221 gctcaccgtc gagatgccgg gcttcatccc ggacgattac gtgcccgacc ccggccagcg
     2281 tctcgagctc tacaagcggc tgtcggccgt cgtcgacgac gatgagctca agagcgtgat
     2341 gaccgaaatc gcggaccgct atggaccggt acccggcgac gcgatcctgc tcggcgagct
     2401 gatgggcatc aaggcgatgg cgcgcaagct cggcgtgctc gcgctcgagg tgtcgtcgac
     2461 ccgtgtcgcg atcgcgttgc ccgacgactc gccgctgcat gccaagattc cgtcgagctg
     2521 gcgccgcctg cccgaccgtc gctacgcggc gaacccgccc gcgcgcgccg cattgctcgt
     2581 gttgctgcag cgcgcgtaaa ctcggcacgt ggggcggccg aagctcgata cgtactggtt
     2641 cgtgttcgcg ctcgcctcgg ctgcgctggc ctcggtcggg ctctacaagc tcgcgactca
     2701 gcgagagccg caactagggt cgcccgacta ctactcggcc cacgtcgacc agtacgaccc
     2761 gtacatgtgg ctgttcttcg cggtgctcgc cggcatcatg gccgcacagc tctgggccaa
     2821 gaaggtcagc cgcatcaagg ttcccgacga catccggcgt gccgagaagc gcaagcgcaa
     2881 gcagctccag cagtgatcgt gcagcgcgag caaggtgccg accaggtgca tgcaccgggt
     2941 gcaggccggc ggctgcgcca ggagtgttcg tgcgttagcg cgccgcgcgg ccggcatggc
     3001 cattgctgtg cacaaagacg ttactcgtgc ccctccgcgt tcgtgataga tggctgctcg
     3061 ccatgaaggc cgcctcgctc gtgcttgctg tgctgctggt cggttgcggc catcacagcc
     3121 tcaacgccga tagcggcctc ggtagcgacg acagcatgag cggggatgac gccaacggca
     3181 gcgacgacgg caataccggc agcgatggcg gtcccggcag cgatggcggt ccgggcagcg
     3241 atggcggtcc cggcagcgat ggcggtaaca ccgacggcaa caccggcggc gatggtggga
     3301 acaataatga tggcggcggc agcggcgacg gcaacaccgg tcccggcgcc tgcggcctca
     3361 cagtctgtgc gaaggcgttc tcgagcggca ccgacggcgc ggcgctcgcg gtcgcggtcg
     3421 acagcgcgaa caacgtgtac atcgccgggc tggccgagag cgatctggtg ttcggcacgc
     3481 acacgatctt ccaactcggt gcgcgtgatg cgttcgtcgt gtcgttcgcg gccgacggta
     3541 cgtaccggtg ggcgaagcgg tggggcaacg gcacgaagct cgccggcgcg tacggcatcg
     3601 ccgtcgatgc gagcaacaat atctacatcg tcggcggcat gaccggcgcg gtcgatttcg
     3661 gcggcggcct cgtcggcacg cagggcatgt tcgtcgcgag cttcgacacg aacggcaact
     3721 atcgctgggc gcgtggcgac ggcggcggca gcggcctgct cgcggtcgcg gtcgcggacg
     3781 gcaaagtgtt cggcatcggt cgtttcctca attcggtgac gttctcgccg acgacgacgc
     3841 tgacgagcgc gggcgcagaa gatgtcgtcg tcgccgcatt cgatacggcg acgggcgggt
     3901 tcgcctgggc gcgcagcgtc ggcaccgcgg cgaccgacat cgagggcggc atcgtcgcgc
     3961 gcaccggcgt gctcgcgttc acgatgtcga gcggcgtcat cggtaccggc gagcggctcg
     4021 tgagctggac gaccgctggt gcagatcgct ggagcacgcc gctgcccgct gcactcgatc
     4081 tacgcggact gtccgtcgat gcagccggca cgctgttcgt cagcggcctg cacaccggcg
     4141 gcatcgacgt cgtgccaaac gcgccctacg tcaacggcga caacgagtac gtgcacgcgt
     4201 acaccgacgg cggctcgacg ggcacgagcg cgtggtcgtg gacgctgtcg gaatcgccgg
     4261 cgcacactgt gaccgcgatc ccgctcacac catcagcgct cgaccccgcg cacgatctcg
     4321 tgctcgccgg cacctacgag ggcacgctct acacgggcag cacgacgctg acgagcggca
     4381 accgcaccat cttcctcgcg aagtactcga cgaccggcac gccgctcgcc gctcgccgtt
     4441 tggacgatgc gagtacggcc gcgaacgagt acgtgcacgg cgtggcgatc gctaccaccg
     4501 gaacgaccta cgtggtcggc gtgtattatg gggcaccgaa ctttggcgac ggcgcactgc
     4561 cgtttgcgtc gcagggcggc gtgatgattc tgcgcgtcgc cccgtgagtt tggcttgttg
     4621 gggcctagcg ctcgtgctac cgaatcaagg taagcatgca aaagagcggg tactttcttg
     4681 tcttcgcagc ggcgctcgca ctgggtgcct gccacaagaa gtccgacgaa gacaaagccg
     4741 cccgcgaggc gcgtgcgcaa ggcgggccga gtgcgccgcc acagtccgct gaggagctga
     4801 agacgccgct cgcgaagatc gatgacgtca cgatcacgct cggcgaattc caggagcgca
     4861 tcaaccgcca gtcgccgtac atccgcgccc gctacacgtc gctcgaacag aagaaggagt
     4921 tcctcgagtc gctcgtacgc ttcgaggtgc tcgcgaagga agcgtatcgc cgcggcctcg
     4981 acaaagatcc cgaagtcgtc cgcacgatga agcaggtgat gatccagaag ctgatgcgcg
     5041 acgagttcga cgcgaaggtc accgccaacg acatccccga cgccgacatg aaggcgtact
     5101 acgacgcgaa cctcaccgag tacgtcaagc ccgaggaggt tcgcgtgtcc gcgatcatcc
     5161 tcaagaacaa ggcgcaggcc gagcgcgtgt tcctcgaggc gaagggcgag gccggcaaga
     5221 cgaacaaggg cttccgcgac ctcgtcatga agtactcgag cgacgaggag acgaagctgc
     5281 gtggcggcga cctgcgttac ctcgagatgc aatcgaagga cgcaccgcct gccgtgatca
     5341 aggctgcgtt cgcactcgtc aacacgggcg acgtctcacc ggtcgtcgat gccggcaacg
     5401 gcacgttcta cgtcctcaag caaaccggcc gccgcaagtc gatcacgaag agcttcgacg
     5461 acgccaaacc gcagattcgc aacaagctct tccgcgaaaa gcgactccag gagcagaaga
     5521 acttcatcga taacctgcgg gccaaggcca agatcgagat caacgagccc aacctggcca
     5581 aggtccgcat cgatacctcc cagaaaaccg gaggggatga cggccacgga catgacctgg
     5641 cggctccccc tcctggccct ccgccaggac atgatctaca accggctccc ccggcccaag
     5701 tgcccgcagg cgctgggcaa cccgtcccct agtttcgagg tcctacccct gtgcggaaac
     5761 ttctggtcct gatcgtaacg ctggcaatcc ccgcggtcgc tgccgcgcaa aagccggcgt
     5821 cgccgaacgc gggcaagaag gtcgggctcg agcgtgtggt cgccgtcgtc aacgactcga
     5881 tcattctgca gagcgagctc gaagcccgcc tcgtcccggt gcgtgccgaa gccgagcaaa
     5941 tccaggatcc gacggagcgc cgccgccgcc tcgccaagct ggcgacgcag gtgctcgacg
     6001 agatggtcaa cgacgagctg atcgtgcagg ccgcggaggc cgcgaagatc gaggtcgaca
     6061 cctccgaagt ccaggctgct ctcgacgaga tcaagcagca gaacaacctc gacgatgctg
     6121 gcctcgcgca ggcgctcgcg tcgcagggct acacggtgca gagctaccgt caggatctgc
     6181 ggcgccagct gcttcgcctc cgtgcggtca accagcttgt cgctcccaag gtccaggtca
     6241 ccgaggacga cgtgcgcagc cgctatgccg agctgcaacg gcgctcgaac gcggtcagcg
     6301 cggtgtacct caaccacatc ctgatcaagc tgccggagca tccgaccgag cagcagatcg
     6361 ctgccgcgaa ggacaaagct gcgcaggcga tccagatggt gaactccggc acgccgttcg
     6421 ctgaagtcgc gaagcagatg tccgacgatc cgaacgtcga gctcggctgg ttccagcgcg
     6481 gcagcatcaa tgccgactgg gaacaagtcg tgttcagcat gcaaaaaggt gagacccgcg
     6541 gcccggtcac cggcccgcaa ggcctccacg tcttcaacgt gagtgacgtg caatcggctg
     6601 cgctcaagcc gtaccccgag atgaaggacc agctgcaacg cgaattgcgg cgacgcgagc
     6661 tcgacaagca gaccgctgct tgg
//
LOCUS       DASUFX010000712         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1086919, whole genome shotgun sequence.
ACCESSION   DASUFX010000712 DASUFX010000000
VERSION     DASUFX010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086919"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV99_28630"
     CDS             <1..189
                     /locus_tag="VFV99_28630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063389.1"
                     /translation="QGFPKEGLCVRAEADCLGGCTHVERAWCVEARCLADQQSCDKVA
                     SLITAQYGTPKKACRTIE"
     gene            complement(221..1201)
                     /locus_tag="VFV99_28635"
     CDS             complement(221..1201)
                     /locus_tag="VFV99_28635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063390.1"
                     /translation="MRWWVIALVLGLATPVFAQPWSQGVSEQKKAAAQKKLEEGNALF
                     LKRNFKEAFDAYQAALTSWNHPAIRFNVVRCLIQLDRPVEAYDELTKALEFGKDPFEE
                     AIYNEALNYQKLLATQIAEIEISCSQQGAKVTLDGQPLLTCPGKEKRRVAPGQHGVVG
                     TKEGFLTKPMDIVVVGGKTEAVDVKLMPLDKAAKVVHRWPGWIPWVVFGGGLAVAGVG
                     GLIQINANDLMDDYDRTIARDCANVGCDLTDAKYDDLNNKRDKAEFRNKIAISVMTVG
                     AVGAIAGGVMLVMNRGRTVYDNSAETRGPVTRLDFVPHDDGGVLTLSGGF"
     gene            complement(1224..2279)
                     /locus_tag="VFV99_28640"
     CDS             complement(1224..2279)
                     /locus_tag="VFV99_28640"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5063391.1"
                     /translation="MRSLAALCLAAMACGGGGAPKEPEKPKAAPVDERKAEKDAKGLI
                     TEIVQSVEHAKTDGLMALLAEPLVVFGPRRTDASQSRSDALVALRATFDAMGKDAKPS
                     VKSDGLSVVASPGGNSAWVVDVMDVEGKPMSAMMVLSNEDDFWVVVAAGIAHTPSMKE
                     VRAELKKDAIVPPGMPGFAKVSDSAEGAVDRFKKGLADPTVWAEDLAKRSDAVVVGPS
                     EGDITRGKKDIEKLWKKRAKVNLRHTSAGEVTAMTTRDGELAWVTAPVVRFADDDEPL
                     PLRLFSVFEKTDGKWTMIALQESLALDEPGIGANFKKVTPPSVKEEEPPPKPKEEPKK
                     KTTTKKKKKKKSKKSDD"
     gene            complement(2282..3388)
                     /locus_tag="VFV99_28645"
     CDS             complement(2282..3388)
                     /locus_tag="VFV99_28645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063392.1"
                     /translation="MGAIDNFLRRFGYAKLDRYGLIRTADDRVISMRPAVLDDGLGAK
                     IVGWTDDDLAAMELEHWGAPKRATAKPIAAPASLHKLPPAPPPLAARTIAPAAPAAPI
                     APIAAPVSRPLPGVAPAPAAVIAPTPKPASAPAPAPVVAAAEEPGEDEWEWEIAMARA
                     RAVETEVARVDLVRASAVVASAIKGKTNPGFPINGKTQTAKPEPEPLPPWPETEPFNE
                     AWVEENDVAPQVMSVMQKKLAVARSTPARGAAAVKPPPGFAPEKTVQQPSPFANEKTR
                     PLIEPAMRSTVIPVPQLPTVADPKQVRPPAYTPYQPKGRTARGTGTGKVNEQTVRTNA
                     APANDDRTSPYITMPDEVKPSGFAHTKRVAAKQR"
     gene            complement(3613..>3851)
                     /locus_tag="VFV99_28650"
     CDS             complement(3613..>3851)
                     /locus_tag="VFV99_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5063393.1"
                     /translation="VARDKLRAAADAIITDLIACAPISVASAKSAIERGYGRPLVEAL
                     EIERECYERTLFSEDRDEGLAAFAEGRPPQYKGR"
BASE COUNT          571 a         1334 c         1218 g          728 t
ORIGIN      
        1 caaggcttcc cgaaggaggg gctttgcgtc cgagctgaag ctgattgcct cggcgggtgc
       61 actcacgtcg agagagcctg gtgtgtggag gctcgctgtc tcgccgatca gcagagctgc
      121 gacaaggtcg cttcgctgat cacggcacaa tacggcacgc ccaaaaaagc atgccgaacc
      181 atcgagtgac tgcgccaggc gtgcaggctg aatccgtagc tcagaagccg ccgctcagcg
      241 tcagcacgcc gccgtcatcg tgcggcacga aatcgagccg cgtcaccggg ccgcgcgtct
      301 cggcgctgtt gtcgtagacc gtccggccgc ggttcatcac gagcatcacg ccaccggcga
      361 tcgcgccgac cgcaccaacg gtcatcacgc tgatcgcgat cttattgcgg aactcggcct
      421 tgtcgcgctt gttgttgagg tcgtcgtact tcgcatcggt caaatcgcag ccgacattcg
      481 cgcagtcgcg cgcgatcgtg cggtcgtaat cgtccatcag gtcgttcgcg ttgatctgga
      541 tcaagccgcc gacaccggcg accgccaagc caccgccgaa cacgacccac ggaatccagc
      601 ccggccagcg gtggacgacc ttcgcggcct tgtcgagtgg catcagcttg acgtcgacgg
      661 cttccgtctt gccgccgacg accacgatgt ccatcggctt cgtcaggaag ccttccttcg
      721 tgccgacgac gccgtgctgg ccgggcgcca cgcggcgctt ctctttgcct gggcacgtca
      781 gcagcggctg accgtcgagc gtgaccttcg cgccctgttg cgagcaactg atctcgatct
      841 cggcgatctg ggtcgcgagc agcttctggt agttcagcgc ttcgttgtag atcgcctcct
      901 cgaacggatc cttgccgaac tcgagcgcct tcgtcagctc gtcataggcc tcgactggac
      961 gatcgagctg gatcaggcag cgcacgacgt tgaagcgaat cgccgggtgg ttccacgacg
     1021 tgagcgcggc ctgataggcg tcgaacgctt ccttgaagtt gcgcttcaag aacagcgcat
     1081 tgccctcttc gagcttcttc tgggccgcgg ccttcttctg ctccgagaca ccttgcgacc
     1141 acggctgcgc gaacacaggc gtagccaacc ccaggacgag cgcgatcacc caccagcgca
     1201 tgcaaccagt atcgcacgca ctctcagtcg tcggacttct tcgacttctt ctttttcttc
     1261 ttcttcgtcg tcgtcttctt cttcggctct tctttgggct tcggcggcgg ctcttcttcc
     1321 ttgaccgacg gcggcgtcac cttcttgaag ttggccccga tgccgggctc gtcgagggcc
     1381 aacgactcct gcagcgcgat catcgtccac ttgccgtcgg tcttctcgaa gacgctgaac
     1441 agacggagcg gcaacggctc gtcgtcgtcg gcaaaccgga ccaccggcgc ggtcacccac
     1501 gcgagctcac cgtcgcgcgt cgtcatcgcc gtgacctcgc ccgcactggt gtggcgcagg
     1561 ttgaccttcg cgcgcttctt ccacagcttc tcgatgtcct tcttgccgcg cgtgatgtca
     1621 ccttcggaag gaccgacgac gactgcgtcg gagcgcttcg cgagatcctc ggcccacacc
     1681 gtcggatcgg cgaggccctt cttgaagcga tcgaccgcgc cttccgcgct gtcggagacc
     1741 ttcgcgaagc ccggcatgcc aggcggcacg atcgcgtcct tcttcagctc ggcacggact
     1801 tccttcatcg atggcgtgtg cgcgatgccc gcagcgacga cgacccagaa gtcgtcctcg
     1861 ttcgacagca ccatcatcgc cgacatcggc ttgccctcga cgtccatcac gtcgacgacc
     1921 cacgccgagt ttccgcccgg cgacgcgacg acgctgaggc cgtcgctctt cacggacggc
     1981 ttcgcgtcct tgcccatcgc atcgaacgtc gcgcgcaatg cgacgagcgc gtcgctgcgt
     2041 gactgcgaag cgtcggtgcg gcgcggaccg aacacgacga gcggctcggc gagcagtgcc
     2101 atgaggccgt cggtcttggc gtgctcgacg gactgcacga tctcggtgat gagacccttc
     2161 gcgtccttct cggccttgcg ctcgtcgaca ggcgcggcct tcggcttctc gggttctttg
     2221 ggggcgccac caccgccgca tgccattgcg gcgaggcaga gcgctgcaag cgagcgcatg
     2281 gttaccgttg tttggcggcg actcgcttcg tgtgtgcgaa tccgctgggc ttcacttcgt
     2341 cgggcatcgt gatgtacggc gacgtccggt cgtcgttcgc gggcgccgcg ttcgtgcgca
     2401 ccgtctgctc gttgaccttg cccgtgcccg tcccgcgcgc ggtgcgcccc ttcggctgat
     2461 acggcgtgta cgccggcggc cgcacctgtt tcggatcggc gacggtcggc agctgcggca
     2521 ccggaatcac ggtcgaccgc atcgccggct cgatcagcgg ccgcgtcttc tcgttcgcga
     2581 acggcgacgg ctgctgcacg gtcttctcgg gtgcgaatcc cggtggcggc ttcaccgccg
     2641 ccgccccacg cgctggcgtg ctgcgcgcga ccgcgagctt cttctgcatc acgctcatca
     2701 cctgcggcgc gacgtcgttc tcctcgaccc acgcttcgtt gaacggctcg gtctccggcc
     2761 acggcggcag cggctcgggc tcgggcttgg ccgtctgcgt cttgccgttg atcgggaagc
     2821 cagggttcgt cttgcctttg atcgccgacg caaccaccgc cgacgctctc accagatcta
     2881 cgcgcgccac ttcggtttcc accgcgcggg cgcgtgccat tgcgatctcc cattcccact
     2941 catcctcccc cggctcctct gcagcggcaa ctactggggc aggtgctgga gcggacgcag
     3001 gttttggcgt gggcgcgatg acggccgcgg gcgcgggtgc aacgcctgga agcgggcgcg
     3061 agacgggtgc agcaatgggt gcgatcggcg ctgcgggtgc ggcgggtgca atcgtgcgcg
     3121 cggcgagcgg cgggggtgcc ggagggagct tgtgcaaact cgcaggagcg gcaattggct
     3181 tagccgtggc tcgcttcgga gccccccagt gctcgagctc catcgccgcg aggtcgtcgt
     3241 cggtccagcc gacgatcttg gcgccgaggc cgtcgtcgag gacggccgga cgcatggaga
     3301 tcacccggtc atcggcggtc cggatcagcc cgtaccgatc cagcttcgcg tacccgaagc
     3361 ggcgaaggaa gttgtcgatg gcacccatgc gacgcctcaa ttgcagcgtg taggccagct
     3421 gacggcggcg atcgcatcgc gcaactccgc agaccctgga tgccactccc gacgcgcggg
     3481 acgaaggacc ccacgacgag gacccgcgcg gggtctcgtc tcccaccctg tgaactcagc
     3541 gggttgggcg agaaattctt gcaaccaacg cgaggctcac acccaccgct agcgactatt
     3601 catcccgatt gctcagcggc ccttgtactg cggcgggcgg ccttccgcga acgcggcgag
     3661 gccttcgtcg cggtcttcgg agaacagcgt gcgctcgtaa cactcgcgct cgatctcgag
     3721 cgcttcgaca agcggtcgac cgtagccgcg ctcgatcgcg ctcttcgcag atgccacgga
     3781 aatcggcgcg cacgcgatca agtcggtgat gatcgcgtcg gccgccgcgc gcagcttgtc
     3841 gcgcgccacg a
//
LOCUS       DASUFX010000713        17117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1364497, whole genome shotgun sequence.
ACCESSION   DASUFX010000713 DASUFX010000000
VERSION     DASUFX010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17117
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364497"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..677
                     /locus_tag="VFV99_28655"
     CDS             168..677
                     /locus_tag="VFV99_28655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063394.1"
                     /translation="MSPTDDFGFSIDLTVRNEWKNIDLLRTSVQNCFIAVFADLDGCH
                     AIAMVTGELLENALKYGDWSNADRAMFRLRVHGREGNIEVSVQNPLKQSDQHAASLQT
                     SLEWLNSFPKPEAAYRARMLQIAQEDSDAAPSKLGLVRIAFEGNCRISAKVDKGAVTV
                     TAVLMREDA"
     gene            complement(674..2572)
                     /locus_tag="VFV99_28660"
     CDS             complement(674..2572)
                     /locus_tag="VFV99_28660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063395.1"
                     /translation="MRRLALVVLVAACTESPQTNTVAFELDAPLANETFWDLPFPSDL
                     RLTSDGRPDLAGFPNQRNLPVVNDLLSVARERRGFPVMPIAWFRFTDGAPVHTLDTLA
                     DTALLVDIDRESPERGTQYPVVAQSLADDSYGSGLVAIAPRPGTVLRASTQYAFVLTR
                     AFAPGVEVPRAFATLAAGKTPDGARAAAAAKLYAPLFEALSADDMLVATVFTTGDEVA
                     LLHARSEAVRAAHTATIGTITRDATNYPGFCSFSATVTLPQFQVGTAPFNSDGRFELD
                     GDTPRQQGELTVPLRIVLPTTQMPQAGWPLWQFFHGSGGASFDLVDDGPSTTPDGDPT
                     PGEGPGAVVARRGIASAAAALPLNPERQPGSGNYDYLNLNNLAAFPYTFQQGVFEQRL
                     LLDALLDVQLTNCDGTPAHFDGNAVVAGGHSMGGMYTNMVSAVEPRIGAVTAFGAGGF
                     WNLMILDTAIIPGSRALLGSVLGVDSEQLTFVHPSLALIELGWEIADPINAMARLSRR
                     PLPGFPARHVYQPIGLDDKFFRNEIFDAAALAYGNQEAGEMVWPGTQDALATDHMQGL
                     LAYPVRANRTTTSVVVQYRDGGILDAHYIHRQLDEVKYQYGCFLASYLRDGVPTVYAP
                     AAISAPCP"
     gene            complement(2681..3460)
                     /locus_tag="VFV99_28665"
     CDS             complement(2681..3460)
                     /locus_tag="VFV99_28665"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX5063396.1"
                     /translation="MCELLGMECNVPTDIVFSFSGLATRGGKAGPHSDGWGLALYDGN
                     VARMFLEPTPAANSPLARFVREHPIKTLLAIAHVRRKTRGAIGLANTHPFSRELWGRH
                     FVFAHNGTVKKAKHLKLGRFKPIGETDSEHAFCALLGALEHEFKDFPRSQADYAEAVA
                     RHAGKIGKDGTFNMLLGDGKQLFARCSTKLHYIVRKAPFKQATLADEDVTVDFAAVTT
                     PNDRVAVIATMPLTRDEVWTAGVPNTLWQFRGGKLVKTLPS"
     gene            complement(3589..4071)
                     /locus_tag="VFV99_28670"
     CDS             complement(3589..4071)
                     /locus_tag="VFV99_28670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063397.1"
                     /translation="MRLGTTRNLLLMLAITACAHSQTAGGGGAGGGQTGGNSAPVPAQ
                     VRKTIDAILGPTANVTSEQEGGVTTYEAAVQTKLEIEVGGAGELQRTEVALPVAALPT
                     AVTAALASKGTIGEAEVVVSPTGVAFEVEIGDDEIVIDATGKILEQHHEVDYKQDDHD
                     "
     gene            4132..5250
                     /locus_tag="VFV99_28675"
     CDS             4132..5250
                     /locus_tag="VFV99_28675"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HEX5063398.1"
                     /translation="MIRALGVFVVLLALASPARAQEDAEKPAPAKVDTKKAQKADTKK
                     ADAKKADAKKKTDAKVGAKADVKDDEPLADDAAAEGSDADVPLTPEEIEASLPPHVKG
                     PQLVDLGSNIEVNLPEGMILLERAEARKMLEKSGDFTDNVLGIVGKVDANWMIVIEFD
                     DIGYVSDKDADQLDANELFKQFQDGNIQQNARRKTLGVPELFLDGWSEMPQYHAVQHH
                     LVWGLKGHSTDGPVINFFTRILGRAGYMSVNLIDSPDAIEQSKKDTAGVLSGIHFKTG
                     YTYEDYREGDKNSGIGLKALVLGGAGIAVVKAAKGGFIIALLLALKKGFIVVIAAIGG
                     FFKWLFGRKKKDDGLDVASMPPSDPPPSDPPPPSDPTV"
     gene            complement(5331..6644)
                     /locus_tag="VFV99_28680"
     CDS             complement(5331..6644)
                     /locus_tag="VFV99_28680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063399.1"
                     /translation="MWPRWCWLGLLGLVSACSGETGVLAVTLTEAPGSTLLDSVQQLR
                     MTLTNPRQVTTADRTAHGFEISLELPASGEDTAILVEGFDAGGALVANGASPAFPTGA
                     LNGRIVIYMAPPLSVGASPAVLSPARSEVAAAPLTYGAIFAGGRLETGPVSDAISIYN
                     AFDHSVVVGLPMPAPRTALALATGGNNAAYMFGGSDDADAPTADLWRFDTNARPNGAY
                     LDYGAKDAFARTGQSAVPLANEQFLITGAPPAQFSGLNGEMIARDEIAALPAAGVSLI
                     ANDGKPTSIFAGPSGVVRFREGTFTTLSIPEAARPDANVVALPGGKVLVVCGTTDAVR
                     IDAASGDAETLPNIPSVAKTGCAAAATSRFLIIAGGTAAGTIDGTVEIFDAATLAPIT
                     TAPLTVPRTNAVAIALPNGQILIASGLTPSNAPVSTLELFTPPVN"
     gene            complement(6824..7105)
                     /locus_tag="VFV99_28685"
     CDS             complement(6824..7105)
                     /locus_tag="VFV99_28685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5063400.1"
                     /translation="MNLKEHDGAVTFDVLVQPRASRAKVGPLHDGRLKVAVTSPPVDG
                     EANAAVIELLAKTLGVSRGAVEVIAGASSRRKTIKVRGVARAAIEELAK"
     gene            complement(7102..7929)
                     /gene="proC"
                     /locus_tag="VFV99_28690"
     CDS             complement(7102..7929)
                     /gene="proC"
                     /locus_tag="VFV99_28690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5063401.1"
                     /translation="MSILAAHTIGFIGAGNMAEAMIRGLVRGGHVTADRIAASAPRQE
                     RLDELRKAYGIDVTTHNREVVQRCGLLVFSVKPQILDKVMREVGDQVKPGTLVVSIAA
                     GVDTETIEESLPDGVRVVRAMPNTPALVGAGATAVAAGKHASTTDLATARALFDAVGI
                     TVELDESHLDAVTGLSGSGPAYIFLILEALADAGVKVGLSRRNAQRLAAQTVMGSAKL
                     LLETDEHPGRLKDMVTSPGGTAIAGLHTLEEGGLRTTLINAVETATKRARELGRHGK"
     gene            complement(7946..9193)
                     /locus_tag="VFV99_28695"
     CDS             complement(7946..9193)
                     /locus_tag="VFV99_28695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5063402.1"
                     /translation="MIPGNADDEDDSRADASGEIRLPATLEATGPVPARAEIAIVGGG
                     VMGLAIAYYLARRGLHDVVVLERGYLAEGASGRNGGGVRQQWSTEINIRLMQESVELC
                     RRFAVDLGVNVWFRQGGYLFLARSAKEVARLEKNIALQNRCGVPTKMLDPSAAQEIVP
                     ELDLTGIVGASYNSTDGILFPWPFLWGYARQAAALGVRIFTQTAVTQLAQASEGGYVI
                     ETSRGTVRARRVINATGAWSPQLARMVGVEVPTYPIRHEICSSEPLKPFLRPMVSELS
                     SGLYCSQSMRGEIVGGVTVPGHGSTYAMGSTLEFLATYSRRLVRLMPILGDIKILRQW
                     AGPYDQSPDGNPIVGPAPNHPDFFLACGFVGHGFMMAPIVGKLYAEWLTGGAKHEIFE
                     RYTLGRFSDGSAAAHEKEDFNIG"
     gene            complement(9270..9572)
                     /locus_tag="VFV99_28700"
     CDS             complement(9270..9572)
                     /locus_tag="VFV99_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5063403.1"
                     /translation="MAGKVVLCRCEDVTLADLEHCVSRGYLDIEEVKRYTGFGTGPCQ
                     GKECLAPMATQLARLTGKKPEEIRPFTSRPPLAPTPIKILARSPDAHRFDDDKTDP"
     gene            complement(9575..10879)
                     /locus_tag="VFV99_28705"
     CDS             complement(9575..10879)
                     /locus_tag="VFV99_28705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5063404.1"
                     /translation="MARRLPALDAARGDAVEVRIADQRVRAFAQESIAAAALASGTRV
                     LSRSLKYHRPRTFFCLEGHCSGCLVRLDGVPNLRACQSPCTPGSEVEGQNAFPSAEVD
                     LLGAVDFLFSRGLDHHTLMTSSSVLNKLANKVVRQLSGLGKLPDKPATELPEVVERNV
                     DVCVIGGGPAGLAAATTCAKAGKRTLLVDDQLRPGGSLRADPRTGREQAEATWAAAVN
                     AGVEVLARSTAIGFFPEDDRGVLAIASPERLFRAHARAWIWATGGYAVNLPFHENDRP
                     GVIAARAVGRLLVDHGILGGDKVCIVEVPEVASDAAALASALTAAGAEVTRVGLADML
                     GVRGRSWVSGVETKHGRVDCDLVAVAAIPSPASEGARQQKCNVTLDPEAGGFRVVVDA
                     HGKTSTDGVWACGDVTGYQGPQVAAADGARVGANVVAALGSE"
     gene            11182..12789
                     /locus_tag="VFV99_28710"
     CDS             11182..12789
                     /locus_tag="VFV99_28710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063405.1"
                     /translation="MPPAQAGAGGNPQPAKTMFGYAAPVVPNRPGQPPAPAAPQQPPR
                     PAAPQQGGFQPPQQQGFQPPQQQGFGAPQGQPAGPSGQNPYGQPGGQNPYGQPAGGQN
                     PYGQPAGGQNPYGQPAGGQNPYGQPAGQNPYGQPAGGQFGGQPGGSGFGQPAGQQPGY
                     GQQPGFGQQPGYPQAANPYAAPQQDLPGPLDDMARKIPTSAPGTIFGFPVAKLRDATI
                     QRKILFLAGVALVASIVVPLMTSPVLVFPFSSGVPKWDFLIWPIISGGAYLLVAAAPP
                     DIRAKVPPAVLQWIPFGVSFAGIFMVGGGGGGGGAGGFAGVHDTLHQLGYAVLVFGLL
                     ARIAQPQDQIARIIIAVGAGMMVIGWFHMFDYAFHFSGVPALLIIYVLLDFIITTLGV
                     ACILFVVPPQKLPPALQAIDAFGPLIAAVLILWLPAQVILFGLAGLVHLHMGIGAILI
                     SAHMLIRIVAYFGVLMMASPAAYEEAKAMFMKKPGGGAPPPSGGGYPPPGGGYPPPGG
                     GYPPPGGGGYPPPQGGGGYPQQGGGWQ"
     gene            12883..13923
                     /locus_tag="VFV99_28715"
     CDS             12883..13923
                     /locus_tag="VFV99_28715"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5063406.1"
                     /translation="MRERGLEFKVGLLILLSSAILVAFIFVLGNFSLRSGFNIYVDYD
                     YIGSLQPGAPVKVSGIKVGKVSDIEFRGGKIDAKLPGPNQRVQVRLTVWIEDRVRDSI
                     RSDAEFFINTAGVLGEMYLEIVPGRDWDKPPIVAGAVIHGLPAVHDPPRTDLVVARLY
                     EVLDGVAGVLHDDRDAIKNLLANSANAVKEANDLLHNNRERIGELIGNGADLAKEAKV
                     TLSKVNNGLGDGRQIGTLVADADQTLKAAQNTMSSLTPSAQALMTDATRVTKIITEER
                     IDKAINAADKAATAAGKAGGLIDNVNGMVTDLRAGKGTAGALLARDDVYSDLRELIRD
                     LKRNPWKFFWKE"
     gene            13994..15061
                     /locus_tag="VFV99_28720"
     CDS             13994..15061
                     /locus_tag="VFV99_28720"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5063407.1"
                     /translation="MSLLAQDERLTRRVGAVTLVLIALSIVFFVFVYDQIEWGKHIRL
                     RIYFHTTGGLREGADFVVAGKAVGKIEAITFVPRGTKPKLLGDEEGVAVKVSIDADDA
                     EHIMYGGDVFVTSRGALSGRYLEIGPAPEPGTLLPDGTVAGPLRGLRAGDEIVGRDPP
                     TLDRVLQRTWDNLTTAAKFAADIRPEMNALTAEVDNLRATLDQIAPDVKLRDDVSALI
                     DEARQTKVALGGDAGIDRISAMLDHLDTTVTQARSTITKLRTSANQLSAGLDSLKTKL
                     GTRGAEAIDKVEAAIDRVRAAIDKVDPLLAQVEALQQSFARGDGSLLKLMHDPEFPED
                     AKELGKILKRQPWKIINRPPK"
     gene            complement(15162..16157)
                     /locus_tag="VFV99_28725"
     CDS             complement(15162..16157)
                     /locus_tag="VFV99_28725"
                     /inference="COORDINATES: protein motif:HMM:NF019840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEX5063408.1"
                     /translation="MRLWVLAFVLVGCAGNLHIDPYAPASGGVIEAGGALEAGAVPDV
                     RCAGAPATGPAQGFRSWRQKLVAKLARPDHRGYDLISATDEPQVLAGKLAYGVIDKSL
                     AHEDVELFACIAGQWQGVGRTRSDGNGRFALALAPAQRLPVGLRDIYASVVADRSGVR
                     FLAYVAPRDSQLVISDVDGTLTASENLFVKAVVLGRNIKPQPGAAAALRAVAERGYQV
                     VYVTARPDRYTDATRQWLGNNGFPRGPVRLADAWFVMPGSETIAYKQHTLKKLGFPIA
                     AGVGNRKSDVAAYAAVGVPADRIFIKLPEYKGELGTALTAGAAVGFGAYSALPVP"
     gene            complement(16242..17003)
                     /locus_tag="VFV99_28730"
     CDS             complement(16242..17003)
                     /locus_tag="VFV99_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5063409.1"
                     /translation="MTAYIEFKGVMKAFGGKPVLKGMDLAVNKGEVMFIIGTSGVGKS
                     VTIKHLIGLLRIDAGEIWFDGSRVDGIPERNLEPLRRRIGMVFQSSTLFDSMTLAENV
                     ALPLRKHKGMRHHAALVEAKKRLAQVYMDEYAERHPAELSDGMRKRAAIARTLALEPD
                     VVLFDEPTTGLDPVSARRVDRLIRELATSLGVTAIVVSHDPPSIFGIADRVAFIYQGR
                     VHVVATPAELRASADPVVQQFINGQSSGPMETPGF"
BASE COUNT         2934 a         5603 c         5774 g         2806 t
ORIGIN      
        1 ttcaactcgt cgacgatcac ggcgctgatc aagctgattc aggtgtgccg caagtccaac
       61 gtgaagctcg tcatggtcta cgaccagagc ctcaagtggc agcggctctc ctttgatgct
      121 cttcgcgtct tcgagaagaa cgatgatctg tttcacctga ggttcgcatg agtccaactg
      181 acgactttgg gttttccatc gatctgaccg ttcgtaacga gtggaagaac atcgatctac
      241 tcagaacgtc ggtacagaac tgtttcatcg cggtctttgc ggacctcgac ggctgccacg
      301 cgatcgcgat ggtgaccggc gagctgctcg agaacgcgct caagtacggc gattggtcga
      361 atgcagaccg cgcgatgttc cgactccgcg tccacgggcg cgaaggcaac atcgaggtca
      421 gcgtgcagaa cccgctgaag cagagcgatc agcacgcggc gagcctgcag acctcgctcg
      481 agtggctgaa cagcttcccg aagcccgagg cggcgtaccg cgcgcgcatg ctgcagatcg
      541 cgcaggaaga cagcgacgcc gcgccgagca agctcggcct cgtgcgcatc gcgttcgaag
      601 gcaactgtcg gatcagtgcg aaggtcgata agggcgcggt caccgtgacc gccgtcctga
      661 tgcgcgaaga cgcttaaggg cagggcgcgc tgatggcggc cggcgcatac accgtcggca
      721 cgccgtcgcg cagatagctc gccaggaagc agccgtactg gtacttcacc tcgtcgagct
      781 ggcgatggat atagtgggca tcgaggatgc cgccgtcgcg gtactgcacg acgaccgacg
      841 tcgtggtgcg gttcgcgcgc actggatacg cgagcaggcc ctgcatgtga tcggtcgcga
      901 gcgcgtcctg cgtgcctggc cacaccatct cgccggcttc ctggttgccg taggcgagtg
      961 cggccgcgtc gaagatctcg ttgcggaaga atttgtcgtc gagcccgatc ggctgataga
     1021 cgtgccgcgc tgggaagccg ggcaacgggc ggcgcgacag ccgcgccatc gcgttgatcg
     1081 gatcggcgat ctcccagccg agctcgatga gtgcgagcga cggatgcacg aacgtgagct
     1141 gctcgctgtc cacgccgagc acgctaccga gtagcgcgcg cgagcccggg atgatcgcgg
     1201 tgtcgaggat catcaggttc cagaagccac cggcaccgaa cgcagtcacc gcgccgatgc
     1261 gcggctcgac ggcgctcacc atgttcgtgt acatgccacc catcgagtgg ccgccggcga
     1321 cgaccgcgtt gccgtcgaag tgcgccggcg tgccgtcgca gttcgtcagc tggacgtcga
     1381 gcagtgcgtc gagaagcagc cgctgctcga acacgccttg ctggaacgtg tacgggaacg
     1441 cggcgaggtt gttgaggttg agatagtcgt agttgccgga gccaggctgg cgctcggggt
     1501 tgagcggcag tgccgccgcg gcactcgcga tgccgcgccg tgcgacgacc gcgccaggac
     1561 cttcgcccgg cgtcggatcg ccgtcgggcg tcgtgctcgg accgtcgtcg acgagatcga
     1621 acgatgcgcc acccgagccg tggaagaact gccagagcgg ccagccggcc tgtggcatct
     1681 gcgtggttgg cagcacgatc cgaagcggca ccgtcagctc gccttgctgg cgtggcgtgt
     1741 cgccatcgag ctcgaaccga ccgtcgctgt tgaacggcgc cgtgccgacc tgaaactgcg
     1801 gcagcgtcac cgtcgccgag aacgaacaga agccaggata attcgtcgcg tcacgcgtga
     1861 tcgttccaat cgtcgcggtg tgcgcagcac gcacggcttc ggagcgcgca tgaagcaacg
     1921 cgacctcgtc gccggtcgtg aacaccgtcg cgacgagcat gtcgtcggcg gacagcgctt
     1981 cgaacagcgg cgcgtagagc ttcgcggcag cggcggcgcg cgcaccgtcg ggcgtcttcc
     2041 ccgcggcgag cgtcgcgaat gcacggggta cttcgacgcc gggtgcgaac gcgcgggtga
     2101 gcacgaacgc gtactgggtg ctagcgcgca gcacggtgcc ggggcgcggt gcgatcgcga
     2161 cgagcccgct gccgtagctg tcgtcggcga gcgattgcgc gacgaccggg tactgcgtgc
     2221 cgcgctcggg cgattcgcga tcgatgtcga cgagcagcgc cgtatcggcg agcgtgtcga
     2281 gcgtgtgcac cggcgcgccg tcggtaaacc ggaaccacgc gatcggcatc accggaaacc
     2341 cgcggcgctc gcgcgcgacc gacaacagat cgttcacgac gggcaggttg cgctggttcg
     2401 gaaacccggc gaggtcaggc cggccatcgc tggtcagccg caggtccgac ggaaacggca
     2461 gatcccagaa cgtctcgttc gcgagtggcg cgtccagctc gaacgcgacc gtgttcgtct
     2521 gtggcgattc ggtgcaggca gcgacgagta cgaccaaggc caggcgacgc atgcctgttg
     2581 gtactgggat ggcggcgtgt gcccgtacaa atagatccgg ccatcgggac ggcggcgtgc
     2641 gtccatacaa atagattcgg ccatcgcgtt ggctactcgc ctagctaggc agcgtcttca
     2701 cgagcttgcc accgcgaaac tgccacagcg tgttcggcac gcctgcggtc cacacctcgt
     2761 cgcgggtcag cggcatcgtc gcgatcaccg cgacgcgatc gttgggcgtc gtgactgcgg
     2821 cgaagtcgac ggtgacgtcc tcgtcggcaa gcgtcgcctg cttgaacggc gccttgcgca
     2881 cgatgtagtg cagcttggtc gagcagcgcg cgaacagctg cttgccgtca ccgagcagca
     2941 tgttgaacgt gccgtcctta ccgattttgc cggcgtggcg agcgaccgct tcggcgtagt
     3001 ccgcctgcga gcgcgggaag tccttgaact cgtgctcgag cgcgccgagc aatgcgcaga
     3061 acgcatgctc gctgtcggtc tcgccgatcg gcttgaagcg gccgagcttg aggtgtttgg
     3121 ccttcttcac ggtgccgttg tgtgcgaaca cgaaatggcg gccccagagc tcgcgagaga
     3181 acggatgcgt gttcgcgagg ccgatcgcgc cgcgcgtctt gcgtcgcaca tgggcgatcg
     3241 cgagcagcgt cttgatcgga tgctcgcgca cgaatctcgc gagcggcgag ttcgcggccg
     3301 gtgttggctc gagaaacatg cgcgcgacgt tgccgtcgta cagcgcgagc ccccagccat
     3361 cgctgtgcgg accggccttg ccgccgcggg tcgcgaggcc agagaagctg aacacgatgt
     3421 cggtcgggac gttgcactcc atcccgagca gctcgcacat ggcggcagat tatcactgcc
     3481 gagagatcgc gaaccgtacg ctgccgtcgg attgattcgc agccgtgatc gtcagcgcca
     3541 tgtgctcggc ccggctcttg gcgaccttcg ccggtcgcgt ggcgcgcatc agtcgtggtc
     3601 gtcctgcttg taatcgacct cgtgatgctg ctcgaggatc ttgccggtcg catcgatcac
     3661 gatctcgtcg tcgccgatct cgacctcgaa cgcgacgccg gtcgggctga cgacgacctc
     3721 ggcttcaccg atcgtgccct tgctggcgag tgctgcggtc acggcggtcg gcagcgcggc
     3781 gaccgggagt gcgacctcgg tgcgctggag ctcgccggca cctccgacct cgatctcgag
     3841 cttggtctgg accgcggctt cgtacgtcgt cacgccgcct tcctgttcgc tcgtgacgtt
     3901 cgcagtcggg ccgaggatcg cgtcgatggt tttgcgcact tgcgcgggta cgggcgcaga
     3961 gttgccgccg gtttgtccac cgccagcgcc gccaccgccg gcggtctgcg agtgagcgca
     4021 cgcggtgatc gcgagcatca agaggaggtt acgcgtcgtg ccgagtctca tgcgtgcaga
     4081 atacagatcg cagatgaatg gcacgttaaa tcgtgctctt atcgtcgccc tatgatccga
     4141 gccctgggag tgtttgtggt gctgctcgcg cttgcttcgc cggcacgtgc acaggaggac
     4201 gccgagaaac ctgcgccggc gaaggtggac acgaagaagg cgcaaaaagc cgacacgaag
     4261 aaggccgacg cgaagaaggc cgacgcgaag aagaagaccg acgcgaaggt cggtgcgaaa
     4321 gcggacgtga aggacgacga gccgcttgcc gatgacgctg cagccgaagg ctcggatgcc
     4381 gatgtgccgc tgacgcccga agagatcgag gcgtcgctac cgccgcacgt caaaggtcca
     4441 cagctcgtcg acctcggcag caacatcgag gtcaacctgc ctgaaggcat gatcctgctc
     4501 gagcgcgctg aagcgcggaa gatgctcgag aagagcggtg acttcacgga caacgtgctc
     4561 ggcatcgtcg gcaaggtcga cgcgaactgg atgatcgtga tcgagttcga tgacatcggt
     4621 tacgtcagcg acaaggacgc ggaccagctc gacgcgaacg agctgttcaa gcagttccag
     4681 gatggcaata tccagcagaa cgcgcggcgc aagacgctcg gcgttcccga gctgttcctc
     4741 gacggctgga gcgagatgcc gcagtaccac gccgtgcagc atcacctcgt gtggggcctc
     4801 aaaggccaca gcacggacgg tccggtcatc aacttcttca cgcgcattct cggccgcgcg
     4861 ggctacatgt ccgtgaacct gatcgatagc cccgatgcga tcgagcagtc gaagaaggac
     4921 accgcgggcg tgctgagcgg catccacttc aagaccggct acacgtacga ggattaccgc
     4981 gaaggcgaca agaacagcgg catcggcctc aaggcgcttg ttcttggcgg tgcgggcatc
     5041 gcggtcgtca aggcagcgaa gggcgggttc atcatcgcgc ttctgctcgc actgaagaag
     5101 ggcttcatcg tcgtgatcgc ggcgatcggt ggcttcttca aatggctgtt cggcaggaag
     5161 aagaaggatg acgggctcga cgtcgcgtcg atgccgccgt ccgatccgcc gccgtccgat
     5221 ccgccgccgc cgtccgatcc gaccgtctaa cgccgaccgt ccgtctgtcc ggcactgcac
     5281 gagtcgtgca gaaaatagtc ggactggtcg cgctggtcgg gctggtcgga ctaattaacg
     5341 ggaggcgtga agagctcgag cgtcgagacc ggcgcgttgc tcggcgtgag cccactcgcg
     5401 attaggatct gaccgttcgg cagcgcgatc gcgacggcgt tcgtgcgcgg aacggtgagc
     5461 ggagcggtcg tgatcggtgc gagcgtcgcc gcgtcgaaga tctcgaccgt gccgtcgatc
     5521 gtgcctgccg ccgtgccacc cgcgatgatc aagaagcgcg acgttgcagc agcggcgcag
     5581 ccggtcttcg cgaccgacgg gatgttcggc agcgtttcgg cgtcccccga tgcggcgtcg
     5641 atccgcacgg cgtcggtggt gccacagacc acgagcacct tgccgccggg caacgcgacg
     5701 acgttggcgt cgggacgcgc agcttcggga atcgataacg tcgtgaacgt gccctcgcga
     5761 aaccgcacga cgccgctcgg gcccgcgaag atcgaagttg gcttgccgtc gttcgcgatg
     5821 agcgagacgc ccgccgcggg gagcgccgcg atttcgtcgc gcgcgatcat ctcgccgttg
     5881 aggcccgaga actgggcggg aggcgcgccc gtgatcaaga actgttcgtt ggcgagcggg
     5941 actgctgatt gcccggtgcg tgcgaacgca tccttcgcgc cgtagtcgag gtatgcgccg
     6001 ttcggtcgtg cgttcgtatc gaagcgccag agatcggcgg tgggcgcgtc tgcgtcgtcg
     6061 ctgccgccga acatgtacgc cgcgttgttg ccgccggtgg cgagcgcgag tgcggtgcgc
     6121 ggcgccggca tcggcagtcc gacgacgacc gagtgatcga acgcgttgta aatgctgatc
     6181 gcatcgctga ccgggccggt ttcgaggcgg ccgccagcga agatcgcacc gtaggtgagc
     6241 ggcgcggccg cgacctccga gcgagcaggc gagaggaccg ccggcgaggc gccaacgctg
     6301 agtggcggcg ccatgtagat cacgatacgg ccgttgagtg cgccggttgg aaacgccggc
     6361 gacgcgccgt tcgcgacaag cgcgccaccc gcatcgaatc cttcgacgag gatcgcggtg
     6421 tcttcgccgg aggccggcag ctcgagcgaa atctcaaagc cgtgcgcggt acggtcggcg
     6481 gtggtgactt gccgcggatt ggtgagcgtc atccgcagct gctgcaccga atcgagcagc
     6541 gtcgagcccg gcgcttccgt cagcgtcacc gcgagcacgc cggtttcacc ggagcacgct
     6601 gatacgagcc cgagcaaacc aagccagcac catctcggcc acatgcaact cgattgcata
     6661 tcgtccgacg cgcgctgcat atcgtccgac gcgcgctgca tatcgtccga cgcgcgctgc
     6721 atatcgtccg actcgcgctg catatcgtcc gactcgcgct gcatatcgtc cgacgcgcgc
     6781 tgcatatcgt ccgacgcgcg ctgtgatgac ccgtgctgcg cgatcacttc gcgagctcct
     6841 cgatggccgc gcgcgcgacg ccacgcacct tgatcgtctt gcgtcgcgac gacgcgccgg
     6901 cgatcacctc gaccgcgccg cgcgagacgc cgagtgtctt cgcgagcagc tcgatcaccg
     6961 cggcgttcgc ttcgccgtcg accggtggcg aggtcaccgc gaccttgagc cggccgtcgt
     7021 gcagcgggcc gaccttagcg cgcgacgcac gcggctggac gagcacgtcg aacgtcacgg
     7081 cgccgtcgtg ctctttcaga ttcatttccc gtgtcggccg agctcgcgcg cgcgcttcgt
     7141 cgcggtctcg acggcgttga tcaacgtggt gcgcagcccg ccttcttcga gcgtgtgcag
     7201 gcccgcgatt gcggtgccgc cgggcgacgt gaccatgtcc ttgaggcggc ccggatgctc
     7261 gtccgtttcg agcaacagct tcgccgagcc catcacggtc tgtgccgcga gccgctgcgc
     7321 gttgcggcgc gacaggccaa ctttcacgcc cgcgtccgcg agcgcttcga gaatcagaaa
     7381 gatgtatgcg gggccggaac ccgacagtcc ggtcacggca tcgaggtggc tctcgtcgag
     7441 ctcgacggtg atgccgaccg cgtcgaacag tgcgcgggcg gtcgcgagat cggtcgtgct
     7501 cgcgtgcttg cccgcggcaa ctgcagtcgc gcccgcaccg acgagtgccg gcgtgttcgg
     7561 catcgcgcgc acgacgcgca cgccatcggg cagcgactct tcgatcgtct cggtatcgac
     7621 gcctgctgcg atcgacacga cgagcgtgcc gggcttcacc tggtcgccga cctcgcgcat
     7681 gaccttgtcg agaatctgcg gtttcaccga gaacacgagc aggccgcagc gctgcacgac
     7741 ctcgcggttg tgcgtggtga cgtcgatgcc gtacgccttg cggagctcgt cgaggcgctc
     7801 ctggcgcgga gcggatgcgg cgatgcgatc tgcagtgacg tgaccgccgc gcacgaggcc
     7861 gcggatcatc gcctccgcca tgttgccggc gccgatgaat ccgatggtgt gggccgcgag
     7921 gatcgacacg cctcgggtgt agcacctatc cgatgttgaa atcttccttc tcgtgcgcag
     7981 ccgccgatcc gtcgctgaat cgccccagtg tgtagcgttc aaaaatctcg tgcttcgcgc
     8041 cgccggtcag ccactcggcg tagagcttgc cgacgatcgg agccatcatg aagccgtggc
     8101 cgacgaagcc acacgcgagg aaaaagtcag gatggttcgg agcggggccg acgatcgggt
     8161 tgccgtccgg cgattggtcg tacggacccg cccactggcg caggatcttg atgtcgccga
     8221 ggatcggcat caggcgtacg agccggcgcg agtaggtggc gaggaactcg agcgtcgacc
     8281 ccatcgcata cgtcgagccg tggccgggca ccgtcacgcc gccgacgatc tcgccgcgca
     8341 tcgactggct gcagtacagc ccgctcgaca gctccgacac catcgggcgc aagaacggct
     8401 tgagcggctc cgacgagcag atttcgtgac ggatcggata cgtcggcacc tcgacgccga
     8461 ccatccgcgc gagctgcggc gaccacgcac cagtcgcgtt gatcacgcgg cgtgcgcgca
     8521 ccgtaccgcg cgacgtctcg atcacgtagc cgccttcact cgcctgcgcg agctgcgtga
     8581 ctgcggtctg cgtgaagatg cgcacgccga gcgcggctgc ttgccgcgcg tagccccaca
     8641 agaacggcca cggaaacaag atgccgtcgg tcgagttgta agacgcgccg acgatgccgg
     8701 tgagatcgag ctcgggcacg atctcctgcg cggcgctcgg gtcgagcatc ttcgtcggga
     8761 cgccgcaacg attttgcagc gcgatgtttt tctcgagtcg cgcgacttct ttcgcgctgc
     8821 gcgcgaggaa caagtagccg ccttgccgga accacacgtt gacgccgagg tcgacggcga
     8881 agcgccggca cagctccacc gactcctgca tcagccggat gttgatctcg gtcgaccatt
     8941 gctggcgcac gccaccgccg ttgcgacccg atgcgccctc ggcgaggtag ccgcgttcga
     9001 gcacgacgac atcgtggagg ccgcggcgcg cgaggtaata cgcgattgcg aggcccatca
     9061 cgccgccgcc gacgatcgcg atctcggcgc gcgcgggcac cgggcccgtc gcttcgagcg
     9121 tcgcgggcag acgaatctcg ccgcttgcat cagcgcggct gtcgtcttcg tcgtctgcgt
     9181 tgccggggat catggatcgg tcttgtcgtc gtcgaagcga tgcgcgggga tcgtggaggt
     9241 cttgtcgtcg tcgatgcgat gcgcggggat catggatcgg tcttgtcgtc gtcgaagcga
     9301 tgcgcgtcgg gcgagcgcgc gaggatcttg atcggcgttg gtgcgagcgg cggccgcgac
     9361 gtgaacggcc gaatttcctc cggcttcttg cccgtcagcc gcgcgagctg cgtcgccatc
     9421 ggcgcgaggc attccttgcc ctggcacggt ccggtgccga agccggtgta gcgcttgacc
     9481 tcctcgatat cgaggtagcc gcgcgacacg cagtgctcga ggtcggcgag cgtgacgtct
     9541 tcacagcgac agagaacgac tttgccggcc atcgctactc gctacccagc gccgcgacga
     9601 cattcgcacc gacgcgggcg ccatccgcgg cagcaacctg tggcccttga tacccggtga
     9661 cgtctccgca agcccacaca ccgtcggtcg acgtcttgcc atgcgcgtcg acgacgacgc
     9721 gaaatcctcc ggcttcggga tcgagcgtga cattgcactt ctgttgtcgt gcgccttccg
     9781 acgccggcga cggaatcgcg gcgaccgcga cgagatcaca atcgacgcgc ccgtgcttcg
     9841 tctcgacgcc gctcacccag ctgcgaccgc gcacgccaag catgtcggca agcccgacgc
     9901 gcgtgacttc ggcacccgcg gcagtgagcg cacttgcgag cgccgctgca tcgctcgcga
     9961 cttccggcac ctcgacgatg cagaccttgt cgccgccgag gatgccgtga tcgacgagca
    10021 agcgaccgac cgcgcgcgca gcgatcacgc cggggcgatc gttctcgtgg aacggcaggt
    10081 tcaccgcgta gccaccggtc gcccagatcc atgcgcgcgc gtgcgcgcgg aacagacgct
    10141 ccggagaggc gatcgcgagc acgccgcgat cgtcctcggg aaagaaaccg atcgcggtcg
    10201 aacgtgcgag cacttccacg cccgcgttca cggctgcggc ccatgtggct tccgcttgct
    10261 cgcgaccggt acgcggatcg gcgcgcaggc tgccacccgg tcgcagctga tcgtcgacga
    10321 gcaacgtgcg cttgccggcc ttcgcgcacg tcgtcgcggc tgcaaggccc gcgggaccgc
    10381 cgccgatcac gcagacgtcg acgttacgct caacaacttc gggcagctcg gtcgcaggtt
    10441 tgtcgggcaa cttgccgagc cccgacagct gacgcacgac cttgttcgcg agcttgttga
    10501 gcaccgacga gctggtcatc agcgtgtggt gatcgagccc gcgcgagaac aagaagtcga
    10561 ccgcgccaag cagatcgact tcggccgatg gaaatgcgtt ctggccttcg acctcggaac
    10621 ccggcgtgca cggcgactga cacgcgcgca ggttcggcac gccatcgagc cgcaccaggc
    10681 agccgctgca atgaccttcg aggcagaaga acgtgcgcgg acggtgatac ttcagcgacc
    10741 gcgacagcac gcgggtaccg ctcgcgagcg ccgcggctgc gatactttct tgcgcgaacg
    10801 cgcggacacg ctggtcggcg atgcgcactt caacggcatc gcctcgtgca gcatcgagag
    10861 ctggaagccg gcgcgccacc gcacactgat agcgcatgcc gggccgtctc tgcgacgatc
    10921 gcccacccca aaaatctgta gcgatttggt ggcaaagcgc cgcgactaaa cagcgcttga
    10981 cgtcggagtt tggccgagga cgtgcaacgg tgatcggccc ccaattcgac ggcggttcgt
    11041 tgcgttcacg gcaacgtgcg ggttaccgtc tgcaggcgaa aggacagggg aaaccgaatg
    11101 gcgactccaa ctaggccgtg cccgtattgc ggcgcggcag tgctgatgaa cgctcccgcg
    11161 tgcggaacgt gcggtcgacc gatgccgcct gctcaagcag gtgccggtgg taatccacaa
    11221 ccggcgaaga cgatgttcgg atacgcggca ccggtcgttc cgaaccgccc gggtcagccg
    11281 ccggcaccag cagcgccgca gcaaccgccc cgtcctgcag caccgcagca aggtggtttt
    11341 cagccgccgc agcagcaggg attccagccg ccgcaacagc agggcttcgg agctccgcaa
    11401 ggtcaaccgg cgggcccctc cgggcagaac ccgtacggcc aaccgggcgg acagaatccg
    11461 tatggccaac cggctggcgg acagaacccg tacggccaac cggcgggcgg acagaaccca
    11521 tacggccaac cggctggcgg acagaaccca tacggccaac cggccggaca gaatccgtac
    11581 ggtcagccgg caggcggaca gttcggtgga cagcccggtg gctctggctt tggtcagccc
    11641 gctggtcagc agccgggcta cggacagcag cccggattcg gacagcagcc tggctatccg
    11701 caggcagcga atccgtacgc agcgccgcag caggacctgc ccggaccgct cgacgacatg
    11761 gcgcggaaga tcccgacgtc tgcgcccgga acgatcttcg gcttcccggt cgcgaagctt
    11821 cgcgacgcga ccatccagcg caagatcctg ttcctcgctg gcgtcgcgct cgtcgcgtcg
    11881 atcgtcgtgc cgctgatgac gagcccggtg ctcgtgttcc cgttctcgag cggcgtgccg
    11941 aagtgggact tcttgatctg gccgatcatc tccggtggcg cgtacctgct cgtcgcagca
    12001 gcgccgcctg acattcgcgc aaaggtgccg cccgcggtcc tgcaatggat accgttcggt
    12061 gtgtcattcg ccggcatctt catggtcggc ggaggtggtg gcggcggtgg cgctggcggc
    12121 ttcgctggtg tgcacgacac gctgcaccag ctcgggtacg ccgtcctcgt gttcggtctg
    12181 ctcgcgcgca tcgcgcagcc gcaagatcag atcgcgcgca tcatcatcgc ggtcggcgcc
    12241 ggcatgatgg tgatcggctg gttccacatg ttcgactacg ccttccactt ctcgggcgta
    12301 ccggcactgc tgatcatcta tgtcctgctc gacttcatca tcacgacgct cggtgtggcc
    12361 tgcatcctgt tcgtcgtgcc gccgcaaaag ttgccgcctg cattacaggc gatagacgcg
    12421 ttcggaccgc tgatcgcagc ggtgctgatc ctgtggctgc ccgcgcaggt gatcctgttc
    12481 ggtctcgccg gcctcgtgca cctgcacatg ggcatcggtg cgatcctgat cagcgctcac
    12541 atgctgattc gcatcgtggc gtacttcggc gtcctgatga tggcgtcgcc ggccgcgtac
    12601 gaagaggcga aggcgatgtt catgaagaag ccgggtggtg gtgcgccgcc gccgagtggt
    12661 ggcggttatc cgcccccggg tggtggctac ccgccgccgg gcggaggcta tccgccgccg
    12721 ggtggtggtg gttacccgcc gccgcagggc ggtggtggct acccgcagca gggcggcggc
    12781 tggcaataag ctactgactc ccggtcaaaa ctgaccgcac tgtgacgagg cccgttgctg
    12841 caaagcaacg ggccgattcg ttttcggatc tatgctctcg ccatgcgtga acgcggcctg
    12901 gagttcaaag tcggcctgct gatcttgttg tcgagcgcga tcttggtcgc gttcatcttc
    12961 gtgctcggca acttctcgtt gcgctccggg ttcaacatct acgtcgacta cgactacatc
    13021 ggcagcctgc agcccggcgc gccggtcaag gtctcgggca tcaaggtcgg caaggtctcc
    13081 gatatcgagt tccgcggcgg caagatcgac gcgaagctgc cgggaccgaa ccagcgcgtg
    13141 caggtgcggc tcaccgtgtg gatcgaagac cgcgtgcgcg actcgattcg cagcgatgcc
    13201 gagttcttca tcaacacggc tggcgtgctc ggcgagatgt acctcgagat cgtgccaggt
    13261 cgcgattggg acaagccgcc gatcgtggca ggcgcagtga ttcacggtct gccggccgtt
    13321 cacgatccgc cgcgcaccga cctcgtcgtc gcgcgtctct acgaggtgct cgatggcgtt
    13381 gccggcgtgc tccacgacga tcgcgacgcg atcaagaatc tgctcgcgaa cagcgcgaac
    13441 gcggtgaagg aagcaaacga cctgctccac aacaaccgcg agcgcatcgg tgagctcatc
    13501 ggcaacggcg ccgatctcgc gaaggaagcg aaggtcacgc tatcgaaggt gaacaatggc
    13561 ctcggcgatg gccgtcagat cggcacgctg gtcgccgacg ccgaccagac gctcaaggcc
    13621 gcgcagaaca cgatgtcgtc gctgacgccg tcggcgcagg cgctgatgac cgacgcgacg
    13681 cgcgttacga agatcatcac cgaggagcgc atcgacaagg cgatcaacgc cgcggacaag
    13741 gcggcgaccg cggcgggcaa ggccggcggc ctgatcgaca acgtcaacgg catggtcacc
    13801 gacctgcgtg caggcaaggg aaccgccggc gcgttgctcg cgcgcgatga cgtctacagc
    13861 gatctgcgcg agctgatccg cgacctcaag cggaatccgt ggaagttctt ctggaaggaa
    13921 tgatcagccc gatgttccag aggacgatct ggatgcgctg gccgaagtcc gaagccagag
    13981 ggccgaagcc cgcatgagcc ttctcgcgca agacgaacgc ctcacgcgtc gagtgggcgc
    14041 ggtgacgctc gtgctgatcg cgctctcgat cgtatttttc gtgttcgtct acgatcaaat
    14101 cgagtggggc aagcacatcc ggctgcgcat ctactttcat acgaccggcg gtctgcgcga
    14161 gggcgcggat ttcgtcgtcg cgggcaaggc ggtcggcaag atcgaggcga tcacgttcgt
    14221 gccgcgcggc acgaagccga agctgctcgg cgatgaagag ggcgtcgctg tgaaggtctc
    14281 gatcgatgcc gacgacgccg agcacatcat gtacggcggc gacgtgttcg tgacgtcgcg
    14341 cggcgcgctg tcaggtcgct acctcgagat cgggccggcg cccgagcccg gcacgctgtt
    14401 gcccgacggc accgtcgcgg gaccgctgcg cggactgcgt gccggcgacg agatcgtcgg
    14461 ccgtgatccg ccgacgctcg atcgcgtgct gcaacgcacc tgggacaatc tgacgacggc
    14521 cgcgaagttc gccgccgata ttcgtcccga gatgaacgcg ctcaccgccg aggtcgacaa
    14581 cctgcgcgcg acgctcgatc agatcgcgcc ggacgtgaag ctgcgcgacg acgtcagcgc
    14641 gttgatcgac gaagcgcggc agacgaaggt ggcgcttggc ggcgacgccg gcatcgaccg
    14701 catcagcgcg atgctcgacc acctcgatac gaccgtcacg caagcgcgca gcacgatcac
    14761 gaagctgcgt acgagcgcga accagctgag cgccggcctc gactcgctga agacgaagct
    14821 cggcacgcgc ggcgccgaag cgatcgacaa ggtcgaggcg gcgatcgatc gcgtgcgcgc
    14881 ggcgatcgac aaggtcgatc cgctgcttgc ccaggtcgag gcgctgcagc agagcttcgc
    14941 gcgcggcgat ggctcgctgc tcaagctgat gcacgacccc gagttccccg aggacgccaa
    15001 ggagctcggc aagatcttga agcgtcagcc gtggaagatc atcaaccggc cgccgaagtg
    15061 atcaaccggc cgccgaagtg atcaaccggc cgcagaaggg atcaaccggc cgcagaagtg
    15121 atcaaccggc cgcagaagtg atcaaccggc cgccaaaggg atcacggaac tggcagggcg
    15181 ctatacgcgc cgaagccgac cgcagcgcca gccgtgagtg cggtaccgag ctcgcccttg
    15241 tactcgggca gcttgatgaa gatgcgatcg gccggcacac cgaccgctgc gtacgcggcg
    15301 acatcactct tgcgattgcc gacgccagcc gcgatcggaa agccgagctt cttcagcgtg
    15361 tgctgcttgt acgcgatcgt ctcgctgccc ggcatgacga accacgcgtc agcgagccgc
    15421 accgggccgc gcggaaagcc gttgttgccg agccattgcc tcgtcgcatc cgtgtagcga
    15481 tccgggcgcg cggtcacgta gacgacctga tagccccgct cggcaaccgc acgcagcgca
    15541 gccgcggcgc ccggctgtgg cttgatgttg cgaccgagca cgaccgcttt gacgaacagg
    15601 ttctccgacg cggtcagcgt gccgtcgaca tccgagatta cgagctgcga gtcgcgcggc
    15661 gcgacatacg cgaggaaccg cacgccgcta cgatcggcga cgaccgatgc atagatatcg
    15721 cgcagaccga ccggtaagcg ctgcgcgggt gcgagcgcga gtgcgaaccg gccgttgccg
    15781 tcgctgcgcg tacggcccac accttgccac tggccggcga tgcacgcgaa cagctcgacg
    15841 tcctcgtgcg cgagcgactt gtcgatcacg ccgtaggcca gcttgcccgc gaggacctgg
    15901 ggctcgtcgg tcgccgagat caggtcgtaa ccgcggtgat cggggcgcgc gagcttcgcg
    15961 acgagcttct gccgccacga ccggaagcct tgcgcgggac cggtcgccgg tgcgccagca
    16021 cagcgaacgt cgggcaccgc gccagcttcg agcgcgccgc cagcttcgat cacgccgccg
    16081 gatgccggcg catacggatc gatgtgcaga ttccccgcgc agccgacgag cacgaacgcg
    16141 agcacccaca gccgcatcgt gcgtactttg accgcgcgcg gcggttgcgc aacaggggga
    16201 ttctctcagt tgccgaagtc tgccgacctg ggtgcgaccg gtcagaagcc cggcgtctcc
    16261 atcggaccgg acgactggcc gttgatgaac tgctggacga ccggatcggc ggacgcgcgg
    16321 agctcggcgg gcgtggcgac cacgtgcacg cgaccctggt agatgaacgc gacgcggtcc
    16381 gcgatgccga agatcgacgg cgggtcgtgc gagaccacga tcgcggtgac gccgagcgaa
    16441 gtcgccagct cgcgaatcag gcggtcgacg cggcgcgcac tcaccgggtc gaggccggtc
    16501 gtcggctcgt cgaacagcac gacgtccggt tccagcgcga gcgtgcgggc gatcgcggcg
    16561 cgcttacgca tgccgtcgga gagctcggcc ggatggcgct cggcgtactc gtccatgtag
    16621 acctgcgcga gccgcttctt cgcctcgacg agcgcggcgt ggtggcgcat gcctttgtgc
    16681 ttgcgcaagg gcagggcgac gttctcggcc agtgtcatcg agtcgaacag ggtcgagctc
    16741 tgaaacacca tgccgatgcg cctgcgcagc ggctcgaggt tgcgctcggg gatgccatcg
    16801 acgcgcgaac cgtcgaacca gatttcgccg gcatcgatgc gaagcaggcc gatcagatgc
    16861 ttgatcgtga ccgacttgcc gacgcccgac gtgccgatga tgaacatcac ctcgcccttg
    16921 ttcaccgcga ggtccatgcc cttcagtacc ggcttgccgc cgaacgcctt catcacgccc
    16981 ttgaactcga tgtaggcggt cacaggtcgg ccccccagag gaacagcgcg gccgccgaga
    17041 tgatgaagtc gagcacgatg acggcgaagc tcgagccgat caccgcagcc gtcgtcgccc
    17101 agccgacgcc ttcgctg
//
LOCUS       DASUFX010000714         9238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_669906,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000714 DASUFX010000000
VERSION     DASUFX010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669906"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..680
                     /locus_tag="VFV99_28735"
     CDS             162..680
                     /locus_tag="VFV99_28735"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX5063410.1"
                     /translation="MTKRRIALISTGGTIEKTYDELSGVLANHVSVLDVMLASLELRG
                     VEVQRVALMNKDSLDMSADDHTLIAETARKLATETSGVVVVHGTDRLAASGDRLVELV
                     GTPATPVVFTGAMRPYELRSTDGLQNLTEALLAVQLLAPGVYVAMHNQVLQFPGVVKD
                     KRAGTFVKEPPR"
     gene            677..1786
                     /locus_tag="VFV99_28740"
     CDS             677..1786
                     /locus_tag="VFV99_28740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063411.1"
                     /translation="MSFDIAHALSGLLEALNNAHVGFVVFRAQNGVAEKVYANQTLVS
                     SLGYSVDEWLATPLWHVIPEDKKTSVMQIFERLIFDAPIPPAIEVSLMHKDGHEVQAE
                     VAAGRVVAGDGARLLVFVVRDMHRQTQLSLLEADRIALVGALAAGFAHETNNPLTSVL
                     LNLRSLRKQMLNNLTDVPQANALRCLDDITTGAERIASNVRALQTLATRSATGKIDLA
                     AVVSAALRLAAPTLEPRAHVIRQIFPVPPVAGEESRIGQAVLAMLLFSSSGFDGELSS
                     ASNRIVVAVEEREGNAVVEVSDNGRDLTGEEALHAFDPFFRSPVRGAGVGVGLGVARS
                     VAITLGGEVTLAQRNGGGAVITMRLPATPDKPPVL"
     gene            complement(1791..2228)
                     /locus_tag="VFV99_28745"
     CDS             complement(1791..2228)
                     /locus_tag="VFV99_28745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5063412.1"
                     /translation="MSLVDRLEQAKKSGDFQALFDIIPYSRFLGLSARLDGGELITTM
                     KYADHLIGNPGLPALHGGTLGALLESSAIFELLWRAETIVLPKTITVTVDYLRSGQPL
                     DTHARSTVTRHGRRVANVRVEAWQTDRAAPVATAHAIFLVMRD"
     gene            complement(2225..2683)
                     /locus_tag="VFV99_28750"
     CDS             complement(2225..2683)
                     /locus_tag="VFV99_28750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5063413.1"
                     /translation="MSEDDKRSERRLQVSRGFVDAIPHNRALGFKVLDIKRAEAVFLL
                     PYDPKLIGNPDTGVLHGGAITALIDACCGAAVFAALTEFSAIATLDLRIDYLRGGDPG
                     KDITCRATCYRLTRNVAFTRAVAYHDDENDAIATSMGTFMLGTKVPGAKK"
     gene            2707..3162
                     /locus_tag="VFV99_28755"
     CDS             2707..3162
                     /locus_tag="VFV99_28755"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5063414.1"
                     /translation="MSRAPIPTWYFAMVVVRRGHRFLLTQEQKYGSTWSIPGGRVEPG
                     ESLVAAAVREVHEETGVPVRIDGILRIEHAASDTTARVRVLFAGSPIDDTPPKTVADA
                     ESLGAAWLTIDEIRQKPLRGAELRAVLESVEAGRQIFPLDLLGQRELSV"
     gene            3164..4234
                     /gene="rsgA"
                     /locus_tag="VFV99_28760"
     CDS             3164..4234
                     /gene="rsgA"
                     /locus_tag="VFV99_28760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5063415.1"
                     /translation="MLQQLGWELYWDDLRRSVDPDCALTPVRIIAEHRGAYHASDSNA
                     VAWVELTGRAFHRAHDKRELPTVGDWVLVERWADAVAGNGAGVIRHVLPRKSFLVRKA
                     AGEATVPQPVAANVDTGIVMTSANKDLSPKRLDRYLTLLRDGGITPVLVLSKVDLVDD
                     PEPMLAELRAVAPSLQTFGVSTVSGAGIDTIRTLGANRTTVLLGSSGVGKSSLLNAVL
                     GEQAQLTRPIRDDERGVHTTTRRELFVTEQGLWIDTPGMRELAQWIDDTDDTAFEDIA
                     ELGEQCKFRDCQHRAEPGCAVRAAVEAGALPADRLASFHKLADERATGAHKQHAARRL
                     AETRKAKAKRYAPRPGKPEDDR"
     gene            4302..5282
                     /locus_tag="VFV99_28765"
     CDS             4302..5282
                     /locus_tag="VFV99_28765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063416.1"
                     /translation="MAIALLAACGDDDSGGGDIPISGLEGAVINAYCNIYVSCGLLDD
                     AATCKQIYTDIEIDPSLVAAVEAGKVIYHPDKARECINGLSSTCDRNTFNRNDNAEAC
                     DQTFEGTVGANGQCAMNEECISQQCDVPSCPDACCQGTCFGDMPPTRPHIGESCGTTG
                     SCVASYCNTTTLICEPYLADGAACTSASQCLNGSCTNQMCVGYPGTGEPCGGTASTLC
                     ANLGDVCNTTSMTCVAYALGGDSCTADRDCSPIYSCGTAGTCQLKPRFGEACVPQTSN
                     DCIDRSYCDPTTNLCTAPKADGGSCTTDRECASNNCDNTTTTCSTLPICI"
     gene            5371..5583
                     /locus_tag="VFV99_28770"
     CDS             5371..5583
                     /locus_tag="VFV99_28770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063417.1"
                     /translation="MSAEDGMRGDSTTILHYDGTQWTRMPVPVGLTVPTDGTLSDVLG
                     RTSDDVFAVGGRLLLHYDGMHWSPMT"
     gene            5713..7329
                     /locus_tag="VFV99_28775"
     CDS             5713..7329
                     /locus_tag="VFV99_28775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5063418.1"
                     /translation="MIAFSSVTKQYGGQILFVDASFQINPGEKVGLVGPNGAGKSTVF
                     RLIVGEEAPDDGNVEKPRKLTLGYFRQDVGDLRGRSILAETCAGAGEVAKLADELAQL
                     SAKLENPGDDLDDVVMRFGEVQARYQDLGGYELEARAQEILHGLGFAQEQLQNDVSTL
                     SGGWKMRVALAQILLARPELLLLDEPTNYLDLESILWLETFLRDYAGTVVMTCHDKEI
                     MNRVVTKIVEIDGGALRTYGGNYDFYEKAREIEAARREAEYARQQAMLAKESRFIERF
                     KTHVAKAAQVQSRLKKLDKIEKIAEPRRFIEKSFEFRAPARSGDDVVRMESLKKAYGT
                     RVVHNGLTLTVRRKERWAVMGENGAGKSTLLKMIAGALAPDDGQAVVGASVSMGYYAQ
                     HVMDLLTSDRTVLEELQEHAPNANQGTLRSLAGAFGFHDDDVFKPISVLSGGEKARVA
                     LAKLLYDAPNLLVLDEPTNHLDITTKRALVNALRDYEGTLIFVSHDRQFLSALATRVL
                     ELSPGNARVYGGNYDEYVASTGREAPGMRA"
     gene            7650..8069
                     /locus_tag="VFV99_28780"
     CDS             7650..8069
                     /locus_tag="VFV99_28780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063419.1"
                     /translation="MNNVGGTPGGMRTFLLGIVMVCVGGYLLMNQVSVHGGYWRFDYF
                     GGYGRSFGITLLPLLFGIGILFFDGRSFAGRFLTGAGALFILAGIIANLDINFRQTSL
                     FNTLVMLILLVGGIGLVVRSVLPMERKSRRDRGGDED"
     gene            8168..8785
                     /locus_tag="VFV99_28785"
     CDS             8168..8785
                     /locus_tag="VFV99_28785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063420.1"
                     /translation="MLMLLAASPTVVRAAGSVASCPNEHCDIPECTALAWIPPDALID
                     TPKIAARVSVAMCAAGKRMSALTLKDDAASRVALNKATAPTFAVLDDLASTGTPDWQI
                     IANALRGQLYVAMTVRLRNSIPPINQAMGPQYAALMARHDRVEQEAQPWLASADDSFR
                     QARQVASVHPELGNNPVVSTAERNSAASLVAARAPAPARPSACCG"
     gene            8857..>9238
                     /locus_tag="VFV99_28790"
     CDS             8857..>9238
                     /locus_tag="VFV99_28790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063421.1"
                     /translation="MGGNDEGGQPPPKRTQFGIAVQTPPGGSPTVRPSAPVMPRPSAP
                     VAMPQPPATSPKTQFGTGPQGPAPARVTPQPIRVPMATTSQAKPVAAAPSQARPVARP
                     VATPVGATSATGYVSDNGVSQQQPS"
BASE COUNT         1586 a         3080 c         3047 g         1525 t
ORIGIN      
        1 gacctcgatc aacgcagcag cgtcacgcag tcgccgacgg tgcccacgag atccgagaaa
       61 ccgcctcgtg ctggaaactt cttactcgcg cttttggtac tgatcgtcgc cggtggggcc
      121 gcagccgtgg tttacttcgc gctgccgtac ttcacctgac aatgacgaag cgacgtatcg
      181 cgttgatctc gaccggtggc acgatcgaga agacctacga cgagctctct ggcgtgctcg
      241 cgaaccacgt ctccgtgctc gacgtcatgc tcgcgtcgct cgagttgcgt ggcgtcgagg
      301 tccagcgcgt cgcgctgatg aacaaagaca gcctcgacat gagcgccgac gatcacacct
      361 tgatcgcgga gaccgcgcgc aagctcgcca ccgagacgtc gggtgtggtc gtcgtccacg
      421 gcaccgatcg gctggcggcg agcggcgatc gtctcgtcga gctggtcggc acgccagcga
      481 cgccggtcgt gttcaccggc gcgatgcgcc cctacgagct gcgctcgacc gatgggctgc
      541 agaacctgac cgaggcacta ctcgcggtac agctgcttgc gcccggcgtc tacgtcgcaa
      601 tgcacaacca ggtgctgcag tttcctggtg tcgtcaaaga caagcgcgcg ggtacgttcg
      661 tcaaggagcc gccgcggtga gcttcgacat cgcgcacgcg ctctcgggct tgctcgaggc
      721 gctcaacaat gcgcacgtcg gcttcgtcgt cttccgcgcg cagaacggcg tcgccgagaa
      781 ggtgtacgcg aaccagacgc tggtatcgag cctcggctac agcgtcgatg aatggctcgc
      841 gactccgctc tggcatgtga tccccgagga caagaagacg tcggtcatgc agatcttcga
      901 gcgcctcatc ttcgatgcgc cgattccgcc cgcgatcgaa gtgagcctga tgcacaagga
      961 cggtcacgaa gtgcaggccg aggtggcagc gggccgcgtc gtggctggcg atggcgcgcg
     1021 gctgctcgtg tttgtcgtgc gcgacatgca ccggcagaca cagctgtcgc tgctcgaagc
     1081 cgaccgcatc gcgctcgtcg gcgcgctcgc cgcggggttc gctcacgaga cgaacaatcc
     1141 gctgacgtcg gtgctgctca atctgcgctc gctgcgcaag cagatgctga acaacttgac
     1201 cgacgtgccg caagcgaacg cgctgcgctg cctcgacgac atcacgacgg gcgccgaacg
     1261 catcgcgagc aacgtgcgcg cgctgcaaac gctcgcgacg cgcagcgcga ccggcaagat
     1321 cgatctcgca gcggtcgtct cggctgcgct gcgactcgcc gcgccgacgc tcgaaccgcg
     1381 cgcacacgtg atccgccaga tctttccggt cccaccggtt gccggcgaag agtcgcgcat
     1441 cggtcaggca gtgctcgcga tgctgctgtt ctcgagctct ggcttcgacg gcgagctgtc
     1501 atcggcgagc aatcgcatcg tcgtcgcggt ggaagagcgc gagggcaacg ccgtcgtcga
     1561 ggtcagcgac aacggccgcg acctcaccgg tgaagaggcg ctccatgcgt tcgatccgtt
     1621 ctttcgctcg cccgtgcgcg gtgccggcgt cggcgtcggt ctcggcgtcg cacgctcggt
     1681 tgcgatcacg ctcggcggcg aagtcacgct cgcgcagcgc aatggcggtg gcgcggtgat
     1741 cacgatgcgc ctgccggcga cacccgacaa gccgcccgtc ctctaggcgc tcagtcgcgc
     1801 atgacgagga agatcgcgtg cgcggtcgcg accggtgcgg cgcgatcggt ttgccacgcc
     1861 tcgacgcgca cgttcgcgac ccgccggccg tggcgggtga cggtgctgcg cgcgtgggtg
     1921 tcgagtggct ggccggaccg taggtaatcg acggtcacgg tgattgtctt gggcagcacg
     1981 atcgtctccg cgcgccagag cagctcgaag atcgccgacg attcgagcag cgcgccgagc
     2041 gtgccgccgt gcagcgcggg caatcctgga tttccgatca gatgatcggc gtacttcatc
     2101 gtcgtgatca gctcgccgcc gtccaggcgt gccgacaggc cgaggaaccg cgagtacgga
     2161 atgatgtcga acagcgcctg gaagtcgccc gacttcttcg cttgctcgag tcggtcgacg
     2221 aggctcattt cttcgctccc gggaccttgg tgcccagcat gaacgtgccc atcgatgtcg
     2281 cgatcgcatc gttctcgtca tcgtggtagg cgaccgcgcg tgtgaacgcg acgttgcggg
     2341 tcagccggta gcacgtcgcg cggcaggtga tgtccttgcc cggatcgcca ccgcgcagat
     2401 agtcgatgcg cagatcgagt gtcgcgatcg cggagaactc ggtgagcgca gcgaacacgg
     2461 cagcgccaca gcacgcatcg atgagcgccg tgatcgcacc gccgtgcagc acgccggtgt
     2521 cagggttgcc gatcagcttc ggatcgtacg gcagcaggaa cacggcttcc gcgcgcttga
     2581 tgtcgaggac cttgaacccg agcgccctgt tatgcggaat cgcgtcgaca aacccgcgcg
     2641 aaacctggag gcggcgttcg ctccgcttgt catcctccga catgcggcca cattataggg
     2701 tccccgatga gccgcgcacc gattccgacc tggtacttcg cgatggtcgt ggtgcgccgt
     2761 ggccaccgat tcctcctcac gcaggagcag aaatacggct cgacgtggtc gatcccggga
     2821 gggcgggtcg aaccgggtga atcgcttgtc gccgccgccg tacgtgaagt ccacgaggaa
     2881 accggcgtcc ccgtgcgcat cgacggcatc ctgcggatcg agcacgccgc gtcggacacc
     2941 acggcgcgtg tgcgcgtgct gttcgcgggt tcgccgatcg atgacacgcc gccgaagacg
     3001 gtcgccgatg ccgagtcgct cggcgcggcg tggctgacga tcgacgagat ccgccagaag
     3061 cccctgcgcg gcgccgagct gcgcgccgtg ctcgagagcg tggaggctgg ccgtcagatc
     3121 ttcccgctcg acctgctcgg tcaacgcgag ctgtcggtct gacatgctcc agcagctggg
     3181 gtgggagctc tactgggacg acctgcggcg cagtgtcgat cccgactgcg cgctgacgcc
     3241 agtgcgcatc atcgcggagc atcgcggcgc ctatcacgcg agcgactcga acgcggtcgc
     3301 gtgggtcgag ctgaccggcc gcgcgtttca tcgtgcgcac gacaagcgcg agcttccgac
     3361 cgtcggcgac tgggtgctcg tcgagcggtg ggcggatgca gtcgccggca atggcgctgg
     3421 cgtgatccgc catgtgctgc cgcgcaagag cttcctcgtg cgcaaagcgg ccggcgaagc
     3481 gaccgtgccc caaccggtcg ccgcgaacgt cgacaccggc atcgtgatga cgtcggcgaa
     3541 caaggatctg tcgccgaagc gcctcgatcg ctacctcacg ctactccgcg acggcggcat
     3601 cacgccggtg ctcgtgctgt ccaaggtcga tctcgtcgac gatcccgagc cgatgctcgc
     3661 cgagctgcgc gcggtcgcgc cgtcgctgca gacgttcggc gtgtcgacgg tgtcaggtgc
     3721 cggcatcgac acgattcgca cgctcggcgc gaaccgcacg accgtgctgc tcggcagctc
     3781 cggtgtcggc aagtcgtcgc tgctcaacgc ggtcctcggc gagcaggcgc agctcacgcg
     3841 gccgatccgc gacgacgaac gcggcgttca cacgaccaca cgccgcgagc tgttcgtcac
     3901 cgagcagggc ctgtggatcg acacgcccgg tatgcgcgag ctcgcgcaat ggatcgacga
     3961 caccgacgac accgcgttcg aagacatcgc ggagctcggc gaacagtgca agtttcgcga
     4021 ttgccagcat cgcgccgagc cgggctgtgc agtccgggcc gcggtggaag cgggcgcgtt
     4081 gcccgccgat cggctcgcga gctttcacaa gctcgccgac gaacgtgcga caggagcgca
     4141 caagcaacac gccgcccgcc ggcttgccga aacccggaaa gcgaaggcaa agcgctacgc
     4201 gccgcgacct ggcaaacccg aggacgaccg ctgattgttt ctcgcgttcc cccacgcgat
     4261 gggcgaggat ctttggtgga ggggatatgc gcagactcgc gttggctatc gctttgctcg
     4321 cggcatgtgg ggacgacgac agtggcggcg gtgacattcc gatcagcggc ctcgaaggcg
     4381 cggtcatcaa cgcgtactgc aacatctacg tgagctgcgg cttgctcgac gacgcggcga
     4441 cctgcaagca gatctacacc gacatcgaga tcgatccgag cctggttgcc gcggtggaag
     4501 ccggcaaggt catctatcac cccgacaagg cgcgcgagtg catcaacggc ctgtcgagca
     4561 cctgcgatcg caacacgttc aaccgcaacg acaacgccga agcgtgtgac cagacattcg
     4621 agggcacggt cggcgcgaac ggccagtgcg cgatgaacga agagtgcatc tcgcaacagt
     4681 gcgacgtgcc gtcgtgcccg gacgcctgct gccagggcac gtgcttcggc gacatgccgc
     4741 caacgcgccc gcacatcggc gaatcgtgcg ggaccacagg gagctgcgtc gcgagttact
     4801 gcaacacgac gacgctgatc tgcgagccct atctcgccga cggcgccgcc tgcacgagcg
     4861 cgagccaatg cctcaacggc tcgtgcacga accagatgtg cgtcgggtat ccgggcacgg
     4921 gtgaaccgtg cggtggcacc gcgtcgacgc tgtgcgcgaa cctcggagac gtgtgcaaca
     4981 cgacgtcgat gacgtgcgtg gcgtacgcgc tcggcggtga ttcgtgcacg gccgaccgcg
     5041 actgctcgcc gatctactcg tgcggcaccg ccggcacgtg ccagttgaag ccgcgattcg
     5101 gcgaggcatg cgtgccgcag acgagtaacg actgcatcga tcggagctac tgcgatccga
     5161 cgacgaactt gtgcactgca ccgaaggcag acggcggctc gtgcaccacc gatcgcgagt
     5221 gtgcgagcaa caattgcgac aacaccacga ccacgtgctc gaccctgccg atctgcatct
     5281 gagttatcgt agttgggtga gggtcgcgct cgtcgcgttg ttgttggtcg ggtgtctgaa
     5341 gaccgacgct gtcgagtgca gcttcgggac gtgtccgccg aagacggcat gcgaggagac
     5401 tcgacgacga tcctccacta cgacggcacg cagtggacgc gcatgccggt cccggtcggg
     5461 ctcacggtac cgaccgacgg cacactgagc gatgtgctcg gcaggacgtc cgacgacgtg
     5521 ttcgcggtcg gtggccgcct gctgctgcac tacgacggca tgcactggtc gcccatgacg
     5581 tgaccgcgct cggacggcgg agccgccctc ggtatcgtca atgaccacct atacatcttg
     5641 ggagacgtcg gaatctcggg acgtctcgtc ggaagattac tgccctgacg gctgtgctat
     5701 acgaacgcgc ccgtgatcgc gttctcctcg gtgaccaagc agtacggcgg acagatcctg
     5761 ttcgtcgacg cctcgtttca gatcaacccg ggtgagaagg tcggcctcgt cggccccaac
     5821 ggcgccggca agagcaccgt gttccggctg atcgtcggcg aggaagcccc agacgacggc
     5881 aacgtcgaga agccgcgcaa gctgacgctc ggctacttcc gtcaggacgt cggtgatctc
     5941 cgcggtcgct cgatcctcgc cgagacctgc gccggtgccg gcgaggtcgc aaagctcgcc
     6001 gacgagctcg cgcagctatc ggccaagctc gagaacccag gcgatgatct cgacgacgtc
     6061 gtgatgcggt tcggtgaggt ccaggcccgc taccaggacc tcggcggcta cgagctcgaa
     6121 gcgcgcgcgc aggagatcct gcacggtctc ggattcgcgc aggagcagct gcagaacgac
     6181 gtcagcacgc tgtcgggcgg ctggaagatg cgcgtcgcgc tcgcgcagat cctgctcgca
     6241 cgtcccgagc tcctgctgct cgacgagcca acgaactacc tcgacctcga gtcgatcctc
     6301 tggctcgaga cgttcttgcg cgactacgcc ggcacggtcg tgatgacgtg ccacgacaaa
     6361 gagatcatga accgcgtcgt caccaagatc gtcgagatcg acggtggcgc gctgcgcacc
     6421 tacggcggca actacgactt ctacgagaag gcgcgcgaga tcgaggcggc gcggcgcgag
     6481 gccgagtacg cacgccagca agcgatgctc gcgaaggaga gccgcttcat cgagcgtttc
     6541 aagacgcacg tcgcgaaggc ggcgcaggtc cagagccggc tcaagaagct cgacaagatc
     6601 gaaaagatcg ccgaaccgcg acgcttcatc gagaagagct tcgagttccg cgcaccagcg
     6661 cgctctggcg acgacgtcgt gcgaatggag agtctcaaga aggcctacgg cacgcgcgtc
     6721 gtccacaacg ggctcacgct gaccgtgcgg cgcaaggagc gctgggccgt gatgggcgag
     6781 aacggtgccg gcaagtcgac gctgctcaaa atgatcgccg gtgcgctcgc gcccgacgat
     6841 ggccaggccg tggtcggtgc gtcggtgtcg atgggctact acgcgcagca cgtgatggat
     6901 ctgctcacca gcgatcgcac cgtgctcgag gagttgcagg agcacgcgcc gaacgcgaac
     6961 cagggcacgc tgcgcagcct cgccggtgcg ttcggcttcc acgacgacga cgtgttcaag
     7021 ccgatctccg tgctctcggg tggcgagaag gcgcgcgtcg cgctcgccaa gctgctctac
     7081 gacgcgccga acttgctcgt gctcgacgag ccgacgaacc acctcgacat cacgacgaag
     7141 cgcgcgctcg tcaacgcact gcgcgactac gaaggcacgc tgatcttcgt ctcgcacgac
     7201 cgccagttct tgagcgcgct cgccacgcgc gtgctcgagc tgtcgccggg taacgcgcgc
     7261 gtctacggcg gcaactacga cgagtacgtc gcttcgaccg gccgcgaagc gcccggcatg
     7321 cgcgcctaag aacgcgcccg ccaacacgag cacgtgcacg tgctcgggcg gcaaagagcc
     7381 gaccgtctcg gcgtttgctc gtggacctag cgtccgtggt cgcaccagtc tctcgggtcg
     7441 ctcgtacttg ttcatcgccg atcccgcgac gctcctgctt cgccgacgac ggcggcctat
     7501 gaccagaggc atcggcgcaa cgctcgtctc cgtgcacgtg cacgtgcacg tgacacgtgc
     7561 acgttcggga gccgggtggc cgcgcctagc acgcacgtgc cacttgcgca cgggttcaac
     7621 cggacgcatg ttcaacatca cacatggaca tgaacaacgt cgggggcacg ccgggtggga
     7681 tgcgcacctt cttgctcggc atcgtcatgg tctgcgtcgg cggatatctg ctgatgaacc
     7741 aggtctcggt ccacggtggg tactggcgct ttgactactt cggcggctac gggcgctcgt
     7801 tcggcatcac gctgctgccg ctgctgttcg gcatcggcat cctgttcttc gacggccgct
     7861 cgttcgcggg ccgtttcctc accggcgccg gcgcactgtt catcctcgcc ggaatcatcg
     7921 caaacctcga catcaacttc cgccaaacgt cgctgttcaa cacactcgtg atgctgatcc
     7981 tgctcgtcgg cggtatcgga ctcgtcgtcc gctccgtgtt gccgatggag cgcaagtctc
     8041 gccgtgaccg cggcggcgac gaggactgac gcgtcagcga ttcgcgcgtg agctgcgggc
     8101 acgagcgatg caccaccagc gtgcgttgct tattggagaa gctcacatgc gtttacgagt
     8161 ccctctgttg ctgatgcttt tggccgcctc gcctaccgta gtgcgcgcgg ctggttcggt
     8221 tgcgtcgtgt ccgaacgagc actgcgatat ccccgagtgc actgcgctcg cgtggatccc
     8281 acccgatgcg ctcatcgaca cgccgaagat cgcagcgcgc gtctcggttg cgatgtgtgc
     8341 cgccggcaag cgcatgagtg cgctcacgtt gaaagacgat gctgcgtcgc gtgttgcact
     8401 caacaaggcc actgcgccta ccttcgcggt gctggatgat cttgcgagta cgggtacgcc
     8461 ggactggcag atcatcgcga acgcgttgcg cggtcaactg tacgtcgcga tgaccgtgcg
     8521 gttgcgtaac tcgattccgc cgatcaacca agcgatgggt ccccagtacg cagcgctgat
     8581 ggcccgccac gaccgcgtcg aacaggaagc ccaaccctgg ctggccagcg cggacgattc
     8641 cttccggcag gcgcggcaag tcgcgtcagt gcatcccgaa ttgggaaaca accctgtggt
     8701 ctcaaccgca gagcgcaata gcgcggcttc cctcgtggcc gcccgcgctc cggcgccagc
     8761 gcgtccgtcg gcctgctgcg gataacgcgc ggcgcctgcg gaatcgtcgt acacgcggcg
     8821 ccggattcga cggaagtcgc atgttattct tgctccgtgg gcggcaacga cgagggagga
     8881 cagcctccgc cgaagcgcac gcaattcggc atcgcagtcc agactcctcc gggtggctct
     8941 ccaaccgtgc gtccgtcggc gcccgtgatg ccgcgaccgt cggcaccggt agcaatgccg
     9001 cagccgccgg cgacgtctcc gaagacgcag ttcggtaccg gaccgcaagg cccggcgcct
     9061 gcccgcgtca cgccgcaacc gatacgcgtg ccgatggcga cgacgtcgca agccaagccg
     9121 gttgccgcgg ccccgtcgca ggccaggccg gttgccaggc cggttgccac gccggtcggc
     9181 gcgacgtcgg cgaccggata cgtcagcgac aacggcgtct cgcaacagca accgagcg
//
LOCUS       DASUFX010000715        13627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_113273,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000715 DASUFX010000000
VERSION     DASUFX010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13627
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113273"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFV99_28795"
     CDS             complement(<1..215)
                     /locus_tag="VFV99_28795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5063422.1"
                     /translation="MARRPASPIALFAEREGPEFRQERSRRSYEALLTAAEELFAVHG
                     YDAIGTPEIAQKAGVSVGTFYRYFEDK"
     gene            297..1331
                     /locus_tag="VFV99_28800"
     CDS             297..1331
                     /locus_tag="VFV99_28800"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5063423.1"
                     /translation="MLQAAEKLAGPSIETLEAILDVTYTWGYQETRQKLRDLYDKAVR
                     AQWISDDVLPWDASVDLEKPMGPEQLMPLFGSDIYNKMTERERKNVNIETFSWTLSQF
                     MHGEQGALLATAQLVDSVKDLDSKLYAASQVVDEARHVDVYNRYVHSKIGFSYPVNPH
                     LKTLLDMILKDSRWDMKFLGMQIMVEGLALAAFGMMRNNTSEPLLRDLTAYVMGDEAR
                     HVAFGVLSLRDYYREQPDSVKKEREDFVYEAARLMRDRFLFQEVWEKQGLPAKECMDI
                     ALHNQGQVMFRQMLFAKIVPAIKKMDLLSDRQRQRFAELGILQFENWADPFADSEHGP
                     SGATSARFQT"
     gene            1347..1745
                     /locus_tag="VFV99_28805"
     CDS             1347..1745
                     /locus_tag="VFV99_28805"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5063424.1"
                     /translation="MLRALAISLCFVAALGCDKGKKKEAPAPKQEAPVTAGTTSADGV
                     RRIDVEANQKGYNPERIAGKPGEKLMLVFKRTYDAECISQLKTPDGTVVDLPINKPVE
                     VAVTVPQTGELTFACGMDMFHGTIVASPNG"
     gene            complement(1879..5079)
                     /locus_tag="VFV99_28810"
     CDS             complement(1879..5079)
                     /locus_tag="VFV99_28810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063425.1"
                     /translation="MSVDAAAEEQFGPYLVYERLGVGGMATVHRALEQGAEGFERMVA
                     LKRLLPHLAEDASFIKSFVREAKLASILNHVNIVQIYELGRVGTAYFISMEYIDGRDL
                     RRVLRHARRVTGPPPINITVGLMAQLCDALDYAHNKVDEEGHPLGLVHRDVSPSNLLI
                     TSGGHLKIIDFGIAKAQSSQLRTQTGRVKGKLAYMAPEAVSGSRDLDSRSDIWACGVI
                     LHELLTARPLFASKNEYQTLLKVQRGDIMPPSTFNQACPAELDTIVFRALARDPDQRY
                     ATAAELREALIAVKRQYALQTGYRDIASWLDWAFGLEPPQSFSNNPDSSGVSDQSSPR
                     QHQRQKTPRPARITEEEEAVEVVWGGGNVESEDGPVVLEDVPDVSGKHQRPRRDTETE
                     SPEVDDIPTPLPSHGTMPSIYRGDGVPQSTRGSDQMKAQRSAPHTIPRSLTGPHPRPR
                     GATSPGVAATTVRQTGQVPRLDAPQLGDRIAAQRARDSAAPENTPRPVADSGPTTNPA
                     AHRLRTEPEVPTNPDSRRLRTDQEGMTPEDLFGVAAAEALERVSQEMDAMSTGTGLRA
                     QTDEDETTLEPSVTPVVAPPTVPVVRFSKQYSTPPASVPVVPSSAGLGELTRAQPSTK
                     PGIAPVRAVTAPPAGPRPIANVRIGETIAARGLKPKRTWLLVLGGIALAGGVATAVAV
                     LGHDKSTTSTADKPTPPPTHETGKVNFVLEPTDAEIKIGGNDVHSGSPWSSELPAGVH
                     QVEIHRSGYKSWLTSIELSANETQTLRVVLEPLTNNVASADASLTITTTPAGLEVVID
                     GHVQSQRTPFKGKLPVGAHKIAVRQNGVDVWQQNLTAEASSDYEFNPSFTADKQRERA
                     QRSPHATSNTATADAAIKGDHQDPSPQWIHPGKEPAPSGAPTPVPAVVAPADKTPTTP
                     ITTPAHEAHAPATITAPVPSPAPAPTKPAPAPTAPPAAPKQNVAAPVTPAPAPAAPPK
                     TAVAPVIVPPNAVKKLSGDVPSIERFKNVELPAALAAKVCIDETGKVSSATILSKVQP
                     RVANDLGETLRTWRYAPHKVNGQAVPACFVVSFKAK"
     gene            complement(5171..5413)
                     /locus_tag="VFV99_28815"
     CDS             complement(5171..5413)
                     /locus_tag="VFV99_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX5063426.1"
                     /translation="MKADDIIAKIRAALPDAEVELQDLTGTADHWKATIVSAAFAGKS
                     LLARHRLVMAALADEMKGPIHALTLDVKTPDEIAAR"
     gene            complement(5437..6627)
                     /gene="kbl"
                     /locus_tag="VFV99_28820"
     CDS             complement(5437..6627)
                     /gene="kbl"
                     /locus_tag="VFV99_28820"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX5063427.1"
                     /translation="MYESAKSVFAATLAEIESAGLWKHERLITSPQAAHITTNGKEVI
                     NFCANNYLGLSSHPRVIAAARKALDDRGYGLSSVRFICGTQDRHKELEAAISKFLRTE
                     DTILYSSCFDANGGLFETVLGEEDAIISDALNHASIIDGIRLCKADRHRYEHDNLDDL
                     EAKLKATQDKRLRMIATDGVFSMDGDIARLDAICDLADKYNALVMVDDSHATGFVGKT
                     GRGSAELRGVMDRIDVYTSTLGKALGGASGGFTSGRKEIIDLLRNRSRPYLFSNTLAP
                     PIVAGSIEALALLTESTALRDRLEANTRKFRAGMMAAGLAIREGTHPITPVMLGDARL
                     AGDMAQRLLGHGIYVIGFSYPVVPKGQARIRVQLSAAHSDEDIDRAITAFTNVGRELG
                     VAKA"
     gene            complement(6639..7307)
                     /locus_tag="VFV99_28825"
     CDS             complement(6639..7307)
                     /locus_tag="VFV99_28825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5063428.1"
                     /translation="MGETVTVTAEDGHTFSAYVAYPEGVRRGSLIVVQEIFGVNRHIR
                     SVVDRFAQDGYVTIAPAMFDRFERDVDMGYTADTVAHGRALKAKITTEMAMTDLAAAF
                     DIVEPHGDVGVVGYCWGGFVAWLAAVYLPGITCAVPYYGGGILEHQDLVPRSPVMAHF
                     GDLDPILPIGTVREFIAKHPAHQMFIYAADHGFNCDDRFTYNQAAADLARERTLAFFR
                     QHVG"
     gene            complement(7307..8281)
                     /locus_tag="VFV99_28830"
     CDS             complement(7307..8281)
                     /locus_tag="VFV99_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5063429.1"
                     /translation="MKILLTGAGGQIGHDLIGALVAQGHEVISTDLAPRPPSHAHASG
                     VDWQRLDVTDAAAVQHTFTEIRPDLVFHLAAILSASGEANPRLAYDVNQTGTWNVLEA
                     SRRAKVKRLIFTSSIAVFGPPPSGALPDPTPDDVALHPTTMYGVTKVSGELLCAYYRS
                     RYDIDCRGVRFPGLISAAMPGGGSSDYALFMYVDAVRKGAYEAFARADTRIPLMYMPD
                     GVRALLELAFADRSKLTRTMYNIAAFSPRADEIAKSVLGVIPDAKFTYAPDPVRQGIL
                     DSWPKAIDDSAARRDWGWQHKYDLDGMTEDLIPRVRRMLDLGAVLTHK"
     gene            8598..9569
                     /locus_tag="VFV99_28835"
     CDS             8598..9569
                     /locus_tag="VFV99_28835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063430.1"
                     /translation="MTDPLIGATVADYVIEALIGEGAMGRVYRAHHRTFTNQRYAVKV
                     LLGDLAASSTMRQRFEREAQNASRLDHPNVVNVLDYGCSGPGMYYLVMDLVEGCSLTT
                     LLQSGPMSADRVIRIAHQLCEGLEHAHSRGVIHRDFKCDNILITGEPGREVARIADFG
                     LAMSTRHQVRLTTTGVACTPAYAAPEQLRGDAIDHRVDLYGLGVTMFEMLSGGYLPFE
                     GDLDATIAAKLAHEAPSIVTRAPNVPPGLVTLVGRLLAHDPGDRPRSTRAVIRALETS
                     LTLPRPPIRTIDMRPLSKRQLRARSLTIRGAVLALAIAGLAVAHALY"
     gene            complement(9570..10583)
                     /locus_tag="VFV99_28840"
     CDS             complement(9570..10583)
                     /locus_tag="VFV99_28840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063431.1"
                     /translation="MRSLGVLALIALAACSGSDDSSSGDVYEFGPFTVAAQQEDTTHC
                     VQITLHNTETVYVNTVELTTGPGFHHSNWFFVPEHIFAGPDGTFTCDERNFDIAAAAA
                     VGGVFFAQSTQSMHEVQAFPPGMAIPLPPKTKLVAQIHLLNRSDEPLTLTPKIALTTL
                     AEADVTKRLAGISMEYHPLSLPPRKQSRFTVDCDLGSLEDPSRDRTLDFNIYYTLAHY
                     HQYGTKMQLEAVKPDNTATTIYTTSNSIGDALGGVLTPPFDITGNSRLRLTCEYYNNT
                     DTMIWYGNGDGEMCVFLAFSDSPWLWGGGVLDETPDPQSGVDQGGVMSFTRQCQLFTR
                     EAY"
     gene            complement(10633..11328)
                     /locus_tag="VFV99_28845"
     CDS             complement(10633..11328)
                     /locus_tag="VFV99_28845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5063432.1"
                     /translation="MTGATERVREEWRARIAAEYTSAAITQQLVLWLIQAGAPPDLID
                     AGLAIVADELVHSRMSHEVYTLAGGTEPPAIDRTQLGISRGNEMLELDILRAAVRVFC
                     LGETVAVPLFKHLRERCTQPSARAALDRILRDEVRHRDFGWDLLDWLCASPLGDRVPE
                     LVSQQLPAMFAELEQSYGTGNPTVAGDSGEMTEAERAWGLAPPREYAAILAQTIEKDY
                     RPRFAARGVVIAM"
     gene            complement(11325..11972)
                     /locus_tag="VFV99_28850"
     CDS             complement(11325..11972)
                     /locus_tag="VFV99_28850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063433.1"
                     /translation="MAARSPRRSFASPFVVTLAAAPACYVQSTPPPQGPPPEPPPVQT
                     AEPQPPPPGPVVANPPHPRPASEPATPPQKPPVVIANPPPPTTQGQPPPPQKPPVVIA
                     NPPPPTTSQPKPPVVVVANPPPPQSQEAKWTVYKAKDGCMAAIKVECPPKATCNPPPP
                     FKYACPDNVSLDKPVTVVTHDGTSCFVEYEMPACPKGVACNPPRPRPVACPTPKR"
     gene            12031..>13627
                     /locus_tag="VFV99_28855"
     CDS             12031..>13627
                     /locus_tag="VFV99_28855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063434.1"
                     /translation="MPITARIALASLLVGLVGCRENSCIDGECPLPCESQIYAGCADG
                     PARYAGRLGDAPVQLRLRYGNGGANDIMLSNGYVTAVFSALDEPNDLAPTGGNLIDFG
                     TPGGIDDTTLTYQLAGILPDDAFAYQTIDFHSDTELASVTVRGTLAGRPDVKVVTHYV
                     LRACESYLRVRSELFNGSADKQAFFIADAMHWGKRRIVPFAPVNGQGYAQPKLKLLEL
                     SDQWSPFDFAAGVDPSNDGPSYAAMSCSTKPLYGVNDLEISALGTPMQYVEPGDSMAL
                     ERLLFVGGFGQGGAPVIDNVLVERAQRFGAPTQSVTGRIVAGGLPFGGDIRRASIVIR
                     QGDRALTSVIPKEDGTFRATVEATGDVTTEVWSFGRKVVEKTGTALGDIEVDEPATVQ
                     LTVETITNGNRRDPAWAIVAFAPDDEPTREASTGTFHTRLKECAPWLGPPDGSSPACN
                     HVIVAPQGTDVEVPAGNYVVIASAGPEYGVDIEHVHLQPGEITPVDIDIEKLDLVPPG
                     WLSADLHVHGRASFDSGIPDEDRV"
BASE COUNT         2173 a         4328 c         4733 g         2393 t
ORIGIN      
        1 tgcttgtcct cgaagtagcg atagaacgtg ccgaccgaga cgccggcttt ctgggcgatc
       61 tcgggcgtgc cgatcgcgtc gtaaccatgc acggcgaaca actcttcggc ggccgtgagt
      121 agcgcctcgt agctacgtcg cgaccgttct tggcgaaact ccggaccctc ccgttcggcg
      181 aacagggcga taggggatgc ggggcggcgc gccatgcctg ttcagaatgc ccttgaccgc
      241 aggcccctgg ctagaacaaa attgaaacaa gtttcacttt tcgtgaggga gtgccgatgc
      301 tgcaagctgc cgagaagcta gcgggtccgt cgatcgaaac gctcgaggcg atcctcgacg
      361 tcacgtacac ctggggctac caggagaccc gccagaagct ccgcgacctc tacgacaagg
      421 cggtgcgcgc gcagtggatc tccgacgacg ttctgccctg ggacgcgagc gtcgatctcg
      481 aaaagccgat gggccccgag cagctgatgc cgctgttcgg ttccgacatc tacaacaaga
      541 tgacggaacg cgagcgcaag aacgtcaaca tcgagacgtt ctcgtggacg ctctcgcagt
      601 tcatgcacgg cgagcagggc gcgctcctcg cgaccgcgca gctcgtcgac tcggtcaaag
      661 acctcgacag caagctctac gcggcgtcgc aggtcgtcga cgaagcgcgc cacgtcgatg
      721 tctacaaccg ctacgtgcac tcgaagatcg ggttctccta tccggtcaac ccgcacctca
      781 agacactgct cgacatgatc ctcaaggaca gccgctggga catgaagttc ctcggcatgc
      841 agatcatggt cgagggtctt gcgctcgccg cgttcggcat gatgcgcaac aacacgtccg
      901 agccgctgct ccgcgatctc accgcgtacg tgatgggcga tgaagcgcgc cacgtcgcgt
      961 tcggcgtgct ctcgctgcgc gactactaca gagagcagcc tgactcggtg aagaaggagc
     1021 gcgaggattt cgtctacgaa gccgcgcgtc tgatgcgcga tcgcttcctg ttccaggaag
     1081 tctgggagaa gcagggcctg ccggcgaagg agtgcatgga catcgcgctc cacaaccagg
     1141 gccaggtcat gttccgccag atgctgttcg cgaagatcgt gccggccatc aagaagatgg
     1201 acctcttgag cgaccggcag cgccagcgct tcgccgagct cggcattctc cagttcgaga
     1261 actgggcgga tccgttcgcc gacagcgagc acggcccgag cggcgcaacc tccgcgcggt
     1321 tccaaactta gtgctacaac cagcgcatgc tgcgtgcgct ggccatctcg ctgtgtttcg
     1381 tcgctgccct cggatgtgac aagggcaaga agaaggaagc ccccgcgccg aagcaggaag
     1441 cgccggtcac cgcgggcacc acgtccgcgg acggcgtccg tcgcatcgac gtcgaggcga
     1501 accagaaggg ctacaacccc gagcgcatcg cgggcaagcc gggcgagaag ctgatgctcg
     1561 tgttcaagcg cacgtacgac gccgagtgca tctcgcagct gaagacgccg gatggcacgg
     1621 tcgtcgacct gccgatcaac aagcccgtcg aggtcgcggt caccgtgccg cagacgggcg
     1681 agctaacgtt cgcgtgcggc atggacatgt tccacggcac gatcgtcgcg tcgccgaacg
     1741 gctgaccgcc tcggcaacga cagcgagctt gcccgtgcgt ggggctggat cgaagaggca
     1801 cgcgagccgt gcgaaatcct cgatcgctta ctcgcaatcc tgtggcgtct cacgcatccg
     1861 cgctgatggc gtcgccgctc acttcgcctt gaagctgacg acgaagcacg ccggcacggc
     1921 ctgaccgttg accttgtgcg gtgcgtagcg ccaggtgcgc agcgtctcgc cgagatcgtt
     1981 ggcgacacgg ggctgcacct tcgacaggat cgtggcgctc gacaccttgc cggtctcgtc
     2041 gatgcacacc ttcgcggcga gcgccgcggg tagctcgacg ttcttgaacc gctcgatgct
     2101 cggcacgtcg ccggagagct tcttgacggc attgggcggc acgatcaccg gcgcaacggc
     2161 ggtcttcggc ggcgcggcag gcgcgggtgc gggcgtgacc ggcgcggcga cgttctgctt
     2221 cggcgcggcg ggtggtgctg ttggcgctgg tgctggcttc gtgggcgcgg gtgcaggcga
     2281 gggcaccggg gccgtgatcg ttgcaggtgc gtgcgcctcg tgcgcgggcg tcgtgatcgg
     2341 tgtggtcggc gtcttgtcgg cgggcgcgac gacagcggga acaggcgtcg gtgcgccgga
     2401 tggcgctggc tctttaccgg ggtggatcca ctgtggcgac gggtcttggt gatcaccttt
     2461 gatggcggcg tccgcggtcg cggtgtttga cgtcgcgtgc ggcgagcgtt gcgcgcgctc
     2521 gcgctgcttg tcggccgtga acgacggatt gaactcgtag tcgctgctcg cttctgcggt
     2581 gaggttctgc tgccagacgt cgacgccatt ctggcgtacg gcgatcttgt gcgcgccgac
     2641 ggggagcttg cccttgaacg gcgtgcgctg cgattgcacg tggccatcga tcacgacttc
     2701 gagaccagcg ggggtggtcg tgatcgtgag cgatgcatcg gcgctcgcga cgttgttcgt
     2761 gagcggctcg agtacgacgc gcagcgtttg ggtttcgttt gcggagagct cgatcgacgt
     2821 gagccacgat ttgtagccgg agcgatggat ctcgacctga tgaacgccgg ccggtagctc
     2881 gctcgaccac ggcgaaccgg agtggacgtc gttgccgccg atcttgatct cggcgtcggt
     2941 cggttcgaga acgaagttga ccttgccggt ttcgtgcgtg ggaggcggcg tcggcttatc
     3001 ggccgtgctc gttgtcgact tgtcgtgacc gagcaccgcg acggcggtcg caacgccgcc
     3061 ggcgagcgcg attccgccga gcacgagcag ccaggtgcgc ttgggcttga ggccgcgcgc
     3121 ggcgatcgtc tcgccgatgc gcacgttcgc gatcggccgc ggcccagcgg gcggggcagt
     3181 gacggcgcgc accggtgcga tgccgggctt cgtcgacggc tgcgcgcgcg tcaactcgcc
     3241 gagaccggcc gagctgggaa cgaccggaac actcgcgggc ggcgtcgagt attgcttcga
     3301 gaagcggacg accggcacgg ttggcggcgc gacgactggc gtcaccgacg gctcgagcgt
     3361 tgtttcgtcc tcgtcggttt gtgcccgtag gccggtgcct gtcgacatcg cgtccatctc
     3421 ttgcgagacg cgctcgaggg cttcggcagc cgcgacgccg aacaggtctt ctggcgtcat
     3481 gccttcctga tcggtgcgga ggcggcgcga gtcgggattc gttggcactt cgggctcggt
     3541 gcgcaggcgg tgtgcagcag gattcgtcgt cggcccactg tcggcgaccg ggcgcggcgt
     3601 gttttccggc gccgcgctat cacgcgcgcg ttgtgcggcg atgcgatcgc caagctgagg
     3661 cgcatcgagg cgcggcactt gtcccgtctg ccttaccgtc gtcgcagcga cgccgggcga
     3721 cgtcgcgccg cgcgggcgcg gatgggggcc ggtaagcgag cgcggaatcg tgtgcggcgc
     3781 ggagcgttgc gccttcatct ggtcgctgcc gcgcgtgctc tgcggcactc cgtcgccgcg
     3841 gtaaatcgac ggcatcgtgc cgtgactcgg cagcggcgtc ggaatgtcgt cgacctcggg
     3901 gctttcggtt tcggtatcgc gccgcgggcg ctgatgcttg ccggagacgt cgggcacgtc
     3961 ttcgagcacg acgggaccgt cctcgctctc gacgttgccg cctccccaca ccacctcgac
     4021 ggcttcttct tcttcggtga tgcgtgccgg gcgtggcgtc ttctggcgct ggtgttggcg
     4081 cggcgagctc tgatcgctga cgcccgacga gtcggggttg ttcgagaagc tctgcggcgg
     4141 ctcgagaccg aatgcccagt cgagccagct cgcgatgtcg cgatagccgg tctgcagtgc
     4201 gtactgacgt ttgacggcga tcaacgcttc gcggagctcg gcggcagttg cgtagcgttg
     4261 gtccggatca cgcgcgagcg cgcggaacac gatcgtgtcg agctcggcgg gacacgcttg
     4321 gttgaacgtc gacggcggca tgatgtcgcc gcgctgcacc ttgagcagcg tctggtattc
     4381 gttcttgctc gcgaacaacg ggcgcgcggt gagcagctcg tgcaggatca cgccacacgc
     4441 ccagatgtcg gagcgcgaat ccagatcgcg actgcccgac acggcttcgg gcgccatgta
     4501 cgcgagcttg cctttgacgc ggcccgtctg cgtgcgcagc tgcgacgact gcgccttcgc
     4561 gataccgaag tcgatgatct tgagatggcc accgctcgtg atgagcaagt tcgacggcga
     4621 gacgtcacga tggacgagcc cgagcggatg cccttcctcg tcgactttgt tgtgggcgta
     4681 gtcgagcgcg tcgcacagct gcgccatcaa accgacggtg atgttgatcg gcggtggccc
     4741 ggtgactcgg cgcgcgtgac gaagaacccg acggagatcg cggccgtcga tgtactccat
     4801 cgagatgaag tacgcagtgc cgacgcgacc gagctcgtag atctggacga tgttgacgtg
     4861 gttcaggatc gacgcgagct tcgcttcgcg cacgaacgac ttgatgaagc tcgcgtcctc
     4921 cgcgaggtga ggcagtagcc gtttgagcgc gaccatgcgc tcgaatccct cggcgccttg
     4981 ttcgagcgcg cgatgcacgg tcgccatacc accaacgccg agccgctcgt acacgaggta
     5041 cgggccgaac tgctcttctg cagcggcatc gaccgacatc tatcccagtg ttgtgagagt
     5101 cgcacaaatt gcgcatgcac cgcatcggga aaagccgcga tttcccggcg cattcggcgc
     5161 gcgctatcgc tcagcgcgct gcgatctcgt ccggtgtttt gacgtctagc gtcagcgcgt
     5221 ggatcggacc cttcatctcg tccgcaaggg ccgccatcac caggcgatga cgcgcgagca
     5281 gcgacttacc cgcgaaagcc gcggatacga tcgtggcctt ccagtgatcg gcggtacccg
     5341 tcaggtcctg gagctcgacc tcggcgtcgg gtagcgcggc gcggatcttc gcgatgatgt
     5401 cgtcggcttt cattggtttg gcgcgttcgg ttccgctcag gctttcgcga cgccgagctc
     5461 gcggccgacg ttagtgaacg ccgtaatggc gcgatcgatg tcttcatcgc tgtgtgccgc
     5521 cgaaagctgc acgcgaatgc gggcttggcc tttgggtacg acagggtagg agaacccgat
     5581 gacgtagatg ccgtggccga ggaggcgctg cgccatgtca cccgccaggc gggcatcgcc
     5641 gagcatgacg ggcgtgatcg ggtgcgtgcc ttcgcgtatg gcgaggccgg ccgccatcat
     5701 ccctgcgcgg aatttgcggg tgttcgcctc gaggcggtcg cgcagcgcgg tcgactcggt
     5761 gagcagcgcg agcgcttcga tcgagcccgc gacgatcggc ggcgcgagcg tgttggagaa
     5821 caggtagggc cgcgaccggt tgcggagcag gtcgatgatc tctttgcgtc ccgacgtgaa
     5881 gccgcccgac gcaccgccga gcgctttgcc gagcgtcgac gtgtagacgt cgatccgatc
     5941 catgacgccg cgcagctcgg cgctgccgcg tccggtcttg ccgacgaagc ccgtcgcgtg
     6001 actgtcgtcg accatgacga gcgcgttgta cttgtcggcg agatcgcaga tcgcgtcgag
     6061 ccgcgcgatg tcgccgtcca tcgagaacac gccgtcggtc gcgatcatgc gcaggcgctt
     6121 gtcctgcgtc gctttgagct tcgcttcgag atcgtcgagg ttgtcgtgct cgtagcgatg
     6181 acgatcggct ttgcacaggc gaatgccatc gatgatcgac gcgtggttga gtgcgtcgga
     6241 gatgatcgcg tcttcttcgc cgagcacggt ctcgaacaga ccgccgttcg cgtcgaagca
     6301 cgacgagtag aggatcgtgt cctcggtgcg gaggaacttc gagatcgccg cttcgagctc
     6361 cttgtggcga tcttgcgtac cgcagatgaa gcgcacgctc gacagaccgt aaccgcgatc
     6421 gtcgagcgct ttgcgcgccg ccgcgatcac gcgcgggtgt gatgacagac cgaggtagtt
     6481 gttcgcgcag aagttgatca cttccttgcc gttcgtcgtg atgtgcgcgg cctgtggcga
     6541 cgtgatcaga cgttcgtgct tccacagccc ggcgctctcg atctcggcga gcgtcgcggc
     6601 gaagacagat ttcgcgctct cgtacatggc gccggagatt acccgacgtg ctggcgaaag
     6661 aaagcgagcg tgcgctcacg tgcaaggtcg gcagcggcct ggttgtacgt gaagcggtcg
     6721 tcgcagttga acccgtgatc tgccgcgtag atgaacatct ggtgggcggg gtgcttggcg
     6781 ataaactcgc ggacggtgcc gatcggcagg atcgggtcca ggtcgccaaa gtgcgccatc
     6841 accgggctgc ggggcacgag atcctggtgc tcgaggatgc cgccgccgta gtaggggacg
     6901 gcgcacgtga tgcccggcag gtacaccgcg gcgagccacg cgacgaagcc gccccagcag
     6961 tagccgacga cgccaacgtc gccgtgcggc tcgacgatat cgaatgccgc cgcgaggtcg
     7021 gtcatcgcca tctcggtcgt gatcttggcc ttgagcgcgc ggccgtgcgc gacggtgtcg
     7081 gccgtgtagc ccatgtccac gtcgcgctcg aaccgatcga acatcgccgg cgcgatcgtc
     7141 acgtagccgt cctgtgcaaa ccgatccacg acactgcgga tgtgacggtt cacgccgaag
     7201 atttcctgca cgacgatgag tgagccgcgt cgcacgccct cgggatacgc gacgtacgcc
     7261 gagaacgtgt gaccgtcttc ggcggtgact gtgacggtct cgcccatcac ttgtgggtca
     7321 gcactgcgcc gaggtcgagc atgcggcgga cgcgagggat gaggtcctcg gtcatgccgt
     7381 cgaggtcgta cttgtgctgc cagccccagt cgcggcgcgc ggcggagtcg tcgatcgcct
     7441 tcggccagct gtcgaggatg ccctggcgca ccggatcggg cgcgtatgtg aacttcgcgt
     7501 cgggaatcac accgagcacg ctcttcgcga tctcgtcggc gcggggcgag aacgcagcga
     7561 tgttgtacat cgtgcgcgtg agcttgctgc gatcggcgaa cgcgagctcg agcaacgcgc
     7621 gcacaccgtc gggcatgtac atgagtggga tgcgcgtgtc ggcacgggcg aacgcctcgt
     7681 acgcaccttt gcgcacggca tcgacgtaca tgaacagcgc gtagtcactc gagccgccgc
     7741 ccggcatcgc cgccgagatc aggccgggga agcgaacgcc gcggcaatcg atgtcgtaac
     7801 ggctgcggta gtacgcgcag agcagctcgc cggagacttt cgtcacgccg tacatcgtcg
     7861 tcggatgcag cgcgacgtcg tcgggagtcg gatcgggcag cgcgccgctc ggcggggggc
     7921 cgaacacggc gatgctcgac gtgaagatca gccgcttcac cttcgcgcgc cgcgacgctt
     7981 cgagcacgtt ccacgtaccg gtctgattga cgtcgtacgc gaggcgcggg ttcgcttcgc
     8041 cactcgccga caagatcgcg gcgagatgga acacgagatc ggggcggatc tcggtgaacg
     8101 tgtgctgcac agcggcagca tcggtgacat cgagccgctg ccagtcgaca cccgacgcgt
     8161 gcgcgtggct cggcgggcgc ggcgcgaggt cggtcgagat gacctcatgg ccttgagcca
     8221 cgagggcgcc gatcaagtcg tgaccgatct ggccgcctgc gccggtgagg aggatcttca
     8281 tgtgcggcgc atcttatcca tcggcgggag tgcttcaagg gcagctccgt gtcatcgcgc
     8341 cgtgcgcggt ggtcctcggc gcgcacagga atcaagcacg ctgtagcgcc ggggcacgaa
     8401 cggcgggtct agaccggcat tggctcgccg gttttcggag cacgaacgtt gctaagccct
     8461 cgaacgtggc aatcgagcgg ctcgctagtg ctacggacca aaccaccggt tccgtacggt
     8521 cctgtagcac gtgcctcgcg gtattccgca ccgacttcga acggtgccct actgacggcg
     8581 gcgagctcat gctggcgatg accgacccgc tgatcggtgc caccgtcgcc gactacgtca
     8641 tcgaggcatt gatcggcgag ggcgcgatgg ggcgcgtgta tcgcgcacat catcggacgt
     8701 tcacgaacca gcgctacgcg gtcaaggtgt tgctcggcga cctcgcggcg agctcgacga
     8761 tgcggcagcg cttcgagcgc gaagcacaga acgcgagccg gctcgatcat ccgaacgtcg
     8821 tcaacgtgct cgattacggc tgcagcggac ccggcatgta ttacctcgtg atggatctcg
     8881 tcgagggctg ctcgctcacc acgctcttgc agagcgggcc gatgtccgcc gatcgcgtga
     8941 ttcgcatcgc gcaccagctg tgcgagggcc tcgagcacgc gcatagccgt ggcgtcatcc
     9001 atcgtgactt caagtgcgac aacatcttga tcaccggaga acccggccgc gaggtcgcgc
     9061 ggattgccga cttcgggctc gcgatgtcga cgcgccacca ggtgcggctg acgacgaccg
     9121 gcgttgcatg cacacccgcg tacgcggcgc ccgagcagct gcgtggcgac gcgatcgatc
     9181 atcgcgtcga tctctacggg ctcggcgtga cgatgttcga gatgctgtcg ggcggctact
     9241 tgccgttcga gggtgacctc gacgcgacaa tcgcggcgaa gctcgcgcac gaagcgccat
     9301 cgatcgtcac gcgcgcaccg aacgtgccgc ccgggctcgt cacgctcgtc ggccgactgc
     9361 tcgctcacga tccaggtgat cggccacgct cgacgcgcgc ggtgatccgc gcactcgaga
     9421 cgtcgctgac gttgccgcgg ccaccgattc gcacgatcga catgcgcccg ctgtcgaagc
     9481 ggcagctgcg cgcacgctcg ctcacgattc gcggcgcggt actcgcgctt gcgatcgccg
     9541 gactcgctgt ggctcacgcg ctgtactaat tagtacgctt cgcgcgtgaa cagctggcac
     9601 tggcgggtga agctcatcac gccgccttga tcgacgccgg actgcggatc gggcgtctcg
     9661 tcgagcacgc cgccgcccca cagccacggc gaatcggaga acgcgaggaa cacgcacatc
     9721 tcgccgtcgc cgttgccgta ccagatcatc gtgtcggtgt tgttgtagta ctcgcacgtc
     9781 aggcgcaggc gcgagttgcc ggtgatgtcg aacggtggcg tcagcacgcc gccgagtgcg
     9841 tcgccgatcg agttgctcgt cgtgtagatc gtcgtcgcgg tgttgtcggg cttcaccgcc
     9901 tcgagttgca tcttcgtgcc gtactgatgg tagtgcgcga gcgtgtagta gatgttgaag
     9961 tcgagcgtgc ggtcgcggct cggatcttcg agcgagccga gatcgcaatc gacggtgaac
    10021 cgcgactgct tgcgcggcgg caagctgagc gggtggtact ccatcgagat gcccgcgagc
    10081 cgcttcgtga cgtcggcttc ggcgagcgtc gtgagcgcga tcttcggcgt gagcgtcagc
    10141 ggctcgtcgg agcggttgag caggtgaatc tgcgcgacca gcttcgtctt cggcggcagc
    10201 ggaatcgcca tgccgggcgg gaacgcctgg acctcgtgca tcgactgcgt cgactgcgcg
    10261 aagaacacac cgccgaccgc ggccgccgcg gcgatatcga agttgcgctc gtcgcacgtg
    10321 aacgtgccgt cggggccggc gaagatgtgc tcggggacga agaaccagtt cgagtgatgg
    10381 aagcccgggc cggtcgtcag ctcgacggtg ttgacgtaga ccgtctcggt gttgtgcagc
    10441 gtgatctgca cgcagtgggt cgtgtcctct tgctgcgcgg cgaccgtgaa cgggccgaac
    10501 tcgtagacgt cgccggacga gctgtcatcc gaacccgaac atgcagctag cgcgatcagc
    10561 gcgaggactc ccaggctccg catggcgacc ctcataccgc tggttggagg gtcgagccaa
    10621 tccccaaaaa ggtcacattg cgatgacaac gccccgtgca gcgaaccgcg gccggtaatc
    10681 tttctcgata gtttgcgcga ggatcgcggc gtactcgcgc ggcggtgcga gaccccacgc
    10741 gcgctcggct tcggtcatct ctccggagtc accggcgacg gtcggattgc cggtgccgta
    10801 gctctgctcg agctcggcga acatcgccgg cagctgctgg gacacgagct cggggacccg
    10861 atcgccgagc ggcgacgcgc ataaccaatc gagcagatcc cagccgaagt cgcgatgacg
    10921 aacttcgtcg cgcaggatgc gatcgagcgc cgcgcgcgcc gacggctggg tgcagcgctc
    10981 gcgcagatgc ttgaacagcg ggaccgcgac ggtctcgccg agacagaaca cgcgcaccgc
    11041 ggcgcgcagg atgtcgagct cgagcatctc gttgccgcgc gagatcccga gctgcgtgcg
    11101 atcgatcgcg ggtggctcgg ttccgccggc cagcgtgtag acctcgtggc tcatccgcga
    11161 atgcacgagc tcgtcggcga cgatcgcgag tcccgcatcg atcagatccg gcggcgcgcc
    11221 agcctggatc agccacagca cgagctgctg cgtgatcgca gcggacgtgt actcggcggc
    11281 gatccgcgcg cgccactcct cgcgaacgcg ctcggtcgcg ccggtcatcg cttcggcgtg
    11341 gggcaggcga cggggcgcgg ccgcggcgga ttgcaggcga cacccttcgg gcacgcgggc
    11401 atctcgtact cgacgaagca gctcgtgccg tcatgcgtga cgacggtgac cggcttgtcg
    11461 agcgacacgt tgtcggggca cgcgtacttg aacggcggcg gcggattgca cgtcgccttc
    11521 ggcgggcatt cgaccttgat cgccgccatg cagccgtcct tcgccttgta gacggtccac
    11581 ttcgcttcct gcgattgcgg cggtggcggg ttcgcgacca cgacgacggg cggcttcggt
    11641 tggctcgtcg tcggtggtgg tgggttcgca atcacaaccg gcggcttttg cggtggcggc
    11701 ggttggcctt gcgtcgtcgg tggcggcgga ttcgcgatca ccacgggtgg cttctgcgga
    11761 ggcgtcgcgg gctcgctggc cgggcgcgga tgcggcgggt tcgcgacgac ggggcccggc
    11821 ggtggcggct gcggctccgc ggtttgcacc ggcggcggct ccggcggcgg gccttgcgga
    11881 ggtggagtcg attgcacgta gcaggcgggt gccgcggcga gcgtgacgac aaacggtgac
    11941 gcaaacgaac gacgaggcga acgcgctgcc atgcgcggac gttatgcctc gatgcccgtg
    12001 cagcgcaacc gttctgatac cttcgcagtg atgccgatta cggcacgaat cgcgctcgcg
    12061 tcattgctcg tggggctcgt aggctgccgc gagaacagct gcatcgacgg tgaatgtccg
    12121 ctgccgtgcg agagtcaaat ctacgccggc tgcgccgacg gtcctgcgcg ttacgccggt
    12181 cggctcggtg acgcgccggt gcagctgcgg ctgcgctacg gcaacggcgg cgcgaacgac
    12241 atcatgctgt cgaacggcta tgtcacggcc gtgttctcgg cgctcgacga gccgaacgat
    12301 ctcgcgccga ccggcggcaa cctgatcgac ttcggcacgc ccggcggcat cgacgatacg
    12361 acgctgacct accagctcgc cggcatcctt cccgacgacg cgttcgcgta tcagacgatc
    12421 gatttccact cggacaccga gctcgcgagc gtgacggtgc gcggcacgct tgccggccgg
    12481 cccgacgtca aagtcgtcac ccactacgtg ctgcgcgcgt gcgagtcgta cctgcgcgtg
    12541 cgctccgagc tgttcaacgg cagcgcggac aagcaagcgt tcttcatcgc cgacgcgatg
    12601 cactggggca agcgccgcat cgtgccgttc gcgccggtga acgggcaggg ctacgcgcag
    12661 ccaaagctga agctgctcga gctgtcggat cagtggtcgc cgttcgactt cgcggccggc
    12721 gtcgatccat cgaacgatgg cccgagctac gcggcgatgt cgtgcagcac gaagccgctg
    12781 tacggcgtga acgatctcga gatctccgcg ctcggcacgc cgatgcaata tgtcgagccc
    12841 ggcgactcga tggcgctcga gcggctgttg ttcgtcggcg gcttcggtca ggggggcgca
    12901 ccggtcatcg acaacgtgct cgtcgagcgc gcgcagcggt tcggggcgcc cacgcagtcg
    12961 gtcacgggac gcatcgttgc aggtggactg ccgttcggtg gcgacatccg ccgcgcgtcg
    13021 atcgtgatcc ggcaaggcga tcgcgcgctg acgtcggtca ttccgaagga ggacggcacg
    13081 ttccgcgcga ccgtcgaggc gaccggcgac gtcaccaccg aggtgtggtc gttcggtcgc
    13141 aaggtcgtcg agaaaacggg cacggcgctc ggcgacatcg aggtcgacga gccggcgacc
    13201 gtacagctca ccgtcgagac gatcacgaac ggaaatcggc gcgatccggc atgggcgatc
    13261 gtcgcgtttg cgcccgacga cgagccgacg cgcgaagcga gcaccggcac gttccacacg
    13321 cggctcaagg aatgcgcacc gtggctcggc ccaccggacg gttcgtcgcc ggcatgcaat
    13381 cacgtcatcg tcgcgccgca gggcaccgac gtcgaagtcc cggcgggcaa ctacgtcgtg
    13441 atcgcgagcg cgggccccga gtacggcgtc gatatcgagc acgtgcacct gcagccgggc
    13501 gagatcacgc cggtcgacat cgatatcgag aagctcgacc tcgtgccgcc aggctggctc
    13561 tctgccgacc tgcacgtgca cggtcgcgcg agcttcgact cgggcattcc ggacgaagat
    13621 cgcgtgc
//
LOCUS       DASUFX010000716        10504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1644593, whole genome shotgun sequence.
ACCESSION   DASUFX010000716 DASUFX010000000
VERSION     DASUFX010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644593"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFV99_28860"
     CDS             <1..398
                     /locus_tag="VFV99_28860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063435.1"
                     /translation="FTSTNPNKALGEPFSVLFSGALPPAASVNSFVDIIPPQQLIAIG
                     AMAGDGSINAEVLAEVVIKGDLNGDEIKSQPFFYPVTVCNDCVVNIVGACPVMGTIRG
                     GNACNPFQDGVVDCCTETSGSVSCPARAM"
     gene            449..817
                     /locus_tag="VFV99_28865"
     CDS             449..817
                     /locus_tag="VFV99_28865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063436.1"
                     /translation="MRATLFAETLRKVVDNVDGALAAVVMGLDGIPVETYVRQTDRVD
                     VNTVGMEFSFIIGQVRKAGDSLQVGGLEELSVKAQRLVLVCRMISPQYFVAIAMAPEG
                     NFGKARFLARLATPALVAQL"
     gene            814..1428
                     /gene="aroQ"
                     /locus_tag="VFV99_28870"
     CDS             814..1428
                     /gene="aroQ"
                     /locus_tag="VFV99_28870"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5063437.1"
                     /translation="MSPAARRAGGRRGRTPARRAPGSRARKPLHQDTPKQQAAPRTSG
                     PIRIALLHGPNLNLLGTRAPEIYGATTLNDIEAEMMKRAEARGAELRSAQTNFEGELV
                     ALIQLARNWADAIVINPGAYTHTSIAVRDAIEAVGLPTVEVHLSNIHAREEFRAVSIT
                     AARCIGQISGFGANSYYLGLDAALSHVEGSRRSHGEAGKRQKRT"
     gene            1457..1936
                     /gene="accB"
                     /locus_tag="VFV99_28875"
     CDS             1457..1936
                     /gene="accB"
                     /locus_tag="VFV99_28875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5063438.1"
                     /translation="MARGLAEIVAVQNLSELIVDTKETTITVRRGGAMIAHGVGMPMQ
                     MPMSMPMPMAAAAPAPQALAAPSAAPAAGAPVAVEDKAHTVTSPFVGTFYRKPNPDSP
                     AYVALNDKVEKGAVLCIVEAMKLMNEIEADVTGTVIGILVEDGAPVEYGQPLFKIAV"
     gene            1939..3309
                     /gene="accC"
                     /locus_tag="VFV99_28880"
     CDS             1939..3309
                     /gene="accC"
                     /locus_tag="VFV99_28880"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5063439.1"
                     /translation="MFKKVLIANRGEIALRVIRACREMGLKTVAVHSTADTDALHVKF
                     ADEAVCIGPPPSKASYLNVPMIISAAEVTGADAVHPGYGFLSENADFADICKKCKLTW
                     IGPPPEAMRLMGDKIRAREAMGKAGVPMLPGTSGINDEITLYEAAERIGFPIILKASA
                     GGGGRGMKIVFEREKLIEAWAMGRTEAQAAFGNPEMFMERYCQRPRHIEIQVVGDKHG
                     HYTHLGERECSIQRRHQKVIEEAPSAALPEETRQKIQQAAVKAISAIGYHNVGTMEFL
                     LDTDNSFYFMEMNTRLQVEHPVTELVHGVDLVREQIRIAAGEPLSESVMPLATGEARK
                     PRGHAIELRVNAEDPNTFAPWPGKITALHMPGGLGVRVDTHVYAGYVVPPNYDSLLAK
                     VIVHDVDRAAAIRRARRCLDEMVIEGPRTNLPFLRRIVNHPEFIRGDFDTGFVARMLA
                     ERASAA"
     gene            3514..6579
                     /locus_tag="VFV99_28885"
     CDS             3514..6579
                     /locus_tag="VFV99_28885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063440.1"
                     /translation="MDAARKFVDKGQIDKAVKEYLRIVQEDPKDVRVWLKIGDLYAKK
                     GSKQDATETYLKVARFYHEQGFFLKAVAVYKQILKLDPRLVDVILKLAELYRQLGLMS
                     DAMQHYESVAAHFHREGNTKEALDTVKKLVDLDPENIATRIKLAELYSKEGLVEEAVL
                     QFNMACEQLRRQGRQDDFIKVAERLLWHKPDNHQLNRELAGLYLRRNDPRRALQKLQQ
                     CFKADPRDAETLGLLALAFQALDQKAKTVSVLKELARIHVENKARDKAGEVYRKILEI
                     VPNDPDALQYLGPQAAAVAPQPPAPTPPPIPAPPTRNTPPPPPLRSGTPIPAAVRQAT
                     AGAKFNITTDLPALDVPPSSRMTGAMPLVDEQSLSGVDFALPEYDDADFSADMEPAPD
                     PRRASAAGERHAEEISKILAETDVYVKYGLHQKAVDHLRRVFSLDSENVEAHERLKDI
                     FISQGREQEAEVELLKLAELIAPSDPDRAEAYLQELLAMNGTHTGAFELARKFQLRVA
                     RMSSVSAEVEYSGGGVAEVDPELDDLDLSSAPPSAMGIARNQMAHGSPAPARPPRPPK
                     GFDDGSFDVELDSQVDAVNEYSSQAAHSTRQVPADDMNALAQQAGYDDEDSAVLVDRP
                     PAGGWNEQQYPPTTYEGAQAYGHSLDDAVDRQLDDEALGHDIDQGVAAELGTRESVSD
                     MPFDPHDAREFDRGVSSAKDGTDAVYANGYAETELANPSATASYDDVSDPLSVSGSYD
                     PYGTDNQQPSYEATVLGGPIVDDVDTPPVGGRAESLVEDDLEEVNFYSDQGMYPEAID
                     SLRGLFDRYPNHPLLMAKLRELESLEQGIEPAIHTPPAGQHSGADVSTGTDALDLDEI
                     EEVSADDMIEEDGEPRPAGKRKPSVMLEKPVDESDADTHYDLGLAYKEMGLHDEAIKA
                     FEKVLRSPGREVQCRVMIGMCHRETNNPNEAIHEFKQGLHANPSDRERLSLYYEIGVT
                     YEAMGDLGEALYYYEGVLKRDPNFADSAQRADAIRIRGTRPSQPADDEI"
     gene            7015..7647
                     /locus_tag="VFV99_28890"
     CDS             7015..7647
                     /locus_tag="VFV99_28890"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063441.1"
                     /translation="MIVDVAAEDEELLERYYRELYLPEFAAQREPLEAWQSALAGDQP
                     YQMFVRVVVERGEILGGISYELYPQSGCGLVTYMVIAPAARRSGWGKRLLFEATKELI
                     ARGALAVLGEVNDPRGTGEWDRLERFQRWGCRVLDARYVQPALGPGLVRDRNLVLIAL
                     PPIRESIAGDVVRTFIAELHEVTERCSPDDEMRTVLDEIPERVRLVELLR"
     gene            7676..9073
                     /gene="fumC"
                     /locus_tag="VFV99_28895"
     CDS             7676..9073
                     /gene="fumC"
                     /locus_tag="VFV99_28895"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5063442.1"
                     /translation="MGTRTESDSFGPIEVDDEHYWGAQTQRSLEHFAIGEEKMPIAVV
                     HALAQIKKAAALVNRDLGLLSDELTKLIVQAAEEVIEGELDDEFPLSVWQTGSGTQTN
                     MNVNEVIAGRANELSGGKRGGKKPVHPNDHVNLSQSSNDAFPTAMHLAAVATVEELLL
                     PAVKQLRGTLDVKAHAFADIIKIGRTHLQDATPLTLGQEMSGWVAQLDAGLAAIRAAL
                     PAVHELALGGTAVGTGLNTHPEYANRVAQAIALSTGRPFVTAPNKFQALASHDALLNL
                     HGAIKTLAASLFKIANDIRWLASGPRSGIGEISIPENEPGSSIMPGKVNPTQCEAMTM
                     VCSQVFGNDVAVTVGGASGNFELNVFKPLIAHAVLQSIRLLGDACESFDEHLARGIEP
                     NRAEITRKVGNSLMLVTALAPHIGYDNAAKVAKKAHKDGTTLKEAAIELGLVSAEDFD
                     RWVKAEAMIGPAARE"
     gene            complement(9033..10277)
                     /locus_tag="VFV99_28900"
     CDS             complement(9033..10277)
                     /locus_tag="VFV99_28900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063443.1"
                     /translation="MAKVDADIEVQRFGFSNEGQGRRLVFPRAQTHKIEGRAGQTTYG
                     AADCTFEGLEGRLDTLRWQAESASVGSAWLRNDAGTYDLQVERAELPHGLRLVRAADA
                     NIELLAPHVTFSELRLTVKGPFGRKAPAAQPAPAPQPSSPVQRQEKLRFLDSLSGRIY
                     TTLKVQLDLPVLGVRTLDQQLRVPIQEGSLDFRALEDSLDWLEGTFLDLKFEDQRLKV
                     QWKVPIFGSGHDLITWALDTDAATLASFGRVPVRSLADFRLPSSNKPPTESEIGRKRG
                     VLKAFALDKIEVALSLLAPRHLDVGAGMIMFGGEDQPGMVDLKVSGAIHDRTAGALKG
                     AIGSVDTTIKDLKIGPMVLSADRLHFDGVEQLEVTFDGFRPTAVTVVVHRVTATNLSI
                     KIGEPRKRPPTLEPPVRSSPPP"
BASE COUNT         1986 a         3406 c         3403 g         1709 t
ORIGIN      
        1 cgttcacgag cacgaatccc aacaaggcgc tcggcgaacc gttcagcgtg ttgttctcgg
       61 gtgcgcttcc cccggccgcg tcggtgaact cgttcgtcga catcattcca ccgcagcagc
      121 tgatcgcgat cggcgcgatg gccggcgatg gttcgatcaa tgcggaagtc ctcgccgagg
      181 tcgtgatcaa gggcgacctc aacggcgacg agatcaagtc gcagccattc ttctatccgg
      241 tcaccgtctg caacgattgc gtcgtcaaca tcgtcggcgc gtgcccggtg atgggcacga
      301 tccgcggcgg caacgcgtgc aacccgttcc aggacggtgt cgtcgactgc tgtactgaaa
      361 cgtcgggctc ggtctcgtgc ccggcacgcg cgatgtaatc ctggccggtt gactggcgcc
      421 gctcggtact ttcgcagtac cttgatcatt gagggccacc ttgttcgctg aaacgctcag
      481 gaaagttgtc gacaatgtcg atggcgcgct cgcggcggtt gtcatggggc tcgacggcat
      541 ccccgtcgaa acctacgttc ggcaaaccga tcgcgtggac gtgaacaccg tcggcatgga
      601 gttctcgttc atcatcgggc aggtacgcaa agcaggcgat agcttgcagg tcggcggcct
      661 cgaggagctg tccgtgaagg cgcagcgtct cgtgctcgtc tgccgcatga tttcgccaca
      721 gtacttcgtc gcgattgcga tggcgcccga aggcaacttc ggcaaagcgc gcttcttggc
      781 gcggctcgcc acgcctgcgc tcgtcgccca gctgtgagcc cggccgctcg ccgcgccggg
      841 ggccgcagag ggcgaacgcc tgccaggcgc gcgcccggtt cgcgtgcgcg caagccactc
      901 catcaggaca cgccgaaaca acaagcggct ccacggacga gcgggccgat ccgcatcgcg
      961 ctgctgcatg ggcccaacct caacttgctc ggcacacgtg ctcccgagat ctacggggcc
     1021 acgaccctca atgacatcga agccgagatg atgaagcgcg ccgaggcgcg cggagcggag
     1081 ctgcgatctg cgcagacgaa tttcgaaggc gagctggtcg cgctgattca actggcgcgc
     1141 aactgggccg acgcgatcgt gatcaatccg ggcgcataca cccatacgtc gatcgccgtt
     1201 cgcgatgcga tcgaagcggt aggtctgcct accgtcgagg ttcacttatc gaatattcac
     1261 gcgcgtgaag aatttcgcgc cgtgtcgatt actgcagcgc gttgtatcgg tcagatcagc
     1321 ggattcggcg caaatagcta ctaccttgga ctggacgccg ccctttctca tgtggaaggt
     1381 tccaggcgct ctcatggcga agcagggaaa aggcagaagc gaacctaacg gcgcggaaga
     1441 acacgagctc gtcacgatgg ctcgcggtct cgccgagatc gtggcggtgc agaacctctc
     1501 ggagctgatc gtcgacacca aggagacgac gatcaccgtc cggcgcggcg gcgcgatgat
     1561 cgctcacggc gtgggcatgc cgatgcagat gcccatgtcg atgcccatgc cgatggctgc
     1621 agcagctccc gctccgcagg cgctggcggc accgagcgcg gctcccgcgg caggtgcacc
     1681 cgttgccgtc gaggacaagg cgcacactgt cacgtcgccg ttcgtcggca cgttctatcg
     1741 caagccgaat cccgattcgc cggcgtacgt cgcgctgaac gacaaagtcg agaagggtgc
     1801 cgtgctctgc atcgtcgagg cgatgaagtt gatgaacgag atcgaagccg atgtcaccgg
     1861 cacggtcatc ggaatcctcg tcgaggacgg tgcgcccgtc gaatacggac aaccgctgtt
     1921 caagatcgcg gtgtagcgat gttcaagaag gttctgatcg cgaaccgagg agaaatcgcg
     1981 ctacgcgtga tccgcgcgtg tcgcgagatg ggcctcaaga cggtcgcggt gcactcgacg
     2041 gccgataccg acgcgctgca cgtgaagttc gccgacgaag cggtgtgcat cggtccgccg
     2101 ccgtcgaagg cgagctactt gaacgtgccg atgatcatct cggcggccga ggtcactggc
     2161 gccgacgccg tgcatcccgg ttatggcttt ctctccgaga acgccgactt cgccgacatc
     2221 tgcaagaagt gcaagctgac gtggatcggt ccgccgcccg aggcgatgcg cctgatgggc
     2281 gacaagatcc gcgcgcgcga agcgatgggc aaggccggcg tgccgatgct gcccggtacg
     2341 tcgggcatca acgacgagat cacgctgtac gaagccgccg agcgcatcgg ctttccgatc
     2401 attctcaagg cgagtgccgg cggcggtggt cgcggcatga agatcgtctt cgagcgcgag
     2461 aagctgatcg aggcgtgggc gatgggacgc accgaagcgc aagccgcgtt cggcaatccc
     2521 gagatgttca tggagcgcta ctgccagcgg ccgcgccaca tcgagatcca ggtcgtcggc
     2581 gacaagcatg gccactacac gcatctcggc gagcgcgagt gctcgatcca gcgccgtcac
     2641 cagaaggtga tcgaggaagc gccgagcgcg gcactccccg aggagacgcg ccagaagatc
     2701 cagcaagcag cggtcaaggc gatctcggcg atcggctacc acaacgtcgg cacgatggaa
     2761 ttcctcctcg ataccgacaa cagcttctac ttcatggaaa tgaacactcg cttgcaggtc
     2821 gagcacccgg tcaccgagct ggtgcacggc gtcgacctcg tgcgcgagca gatcaggatc
     2881 gcggcgggcg agccgctgtc ggagtcggtg atgccgctcg cgacgggcga ggcgcgcaag
     2941 ccgcgcggtc atgcgatcga gttgcgcgtg aacgccgagg acccgaatac gtttgcgcca
     3001 tggccgggca agatcacggc gcttcacatg ccgggtggcc tcggtgtgcg tgtagatacg
     3061 cacgtgtacg cgggctatgt ggttccgccg aactacgact cgctcttagc gaaggtgatc
     3121 gtgcacgacg tcgaccgagc cgccgcgatt cgtcgcgcac ggcgctgcct cgacgagatg
     3181 gttatcgaag gaccgcgcac caacctcccg ttcttgcgcc ggatcgtgaa tcacccggag
     3241 ttcatccgag gcgatttcga caccggcttc gtcgcgcgca tgcttgccga gcgagcgtca
     3301 gctgcctgac cggaatctca gctgagaact ggtgatccag tgtgcgcagg tccgacgggg
     3361 taacgcccgg gattccttcg cccttttcct tgaccgcact gccagcggag ccgtactctt
     3421 tcaaagatgc gcggggcata ctgccgtgtg cctaacgtga tgaatttcac ccggaggctg
     3481 taatcgaccg tggccttcaa caaagaaaag gtcatggatg cggcccggaa gtttgtcgat
     3541 aaggggcaaa tcgacaaagc ggtgaaggag tacctgcgga tcgtccaaga agatccgaag
     3601 gacgtccgcg tctggctgaa gatcggcgac ctctacgcga agaagggctc gaagcaagac
     3661 gcgacggaga cgtatctcaa agtcgcgcgc ttctatcacg agcaaggttt cttcctcaaa
     3721 gccgtcgccg tctacaagca gatcctcaag ctcgatccgc gtctcgtcga cgtgatcctg
     3781 aagctcgccg agctctatcg ccagctcggg ttgatgagcg atgcgatgca gcactacgag
     3841 tccgtcgctg cgcacttcca tcgcgaaggc aacacgaagg aagcgctcga caccgtcaag
     3901 aagctcgtcg atctcgatcc cgaaaacatc gcgacgcgca tcaagctcgc cgagctctac
     3961 tcgaaggaag gcctcgtcga ggaagcggtg cttcagttca acatggcgtg cgagcagctg
     4021 cgccgccaag gtcgtcaaga cgacttcatc aaggtcgcgg agcgcttgct ctggcacaag
     4081 ccagacaacc atcagctcaa ccgcgagctc gccgggctct acttgcgccg caacgatccg
     4141 cgtcgcgcgc tgcaaaagct ccagcagtgc ttcaaagcgg atccacgcga cgctgaaacg
     4201 ctcggcctcc tcgcgctcgc attccaagca ctcgaccaga aagcaaagac cgtctcggtg
     4261 ctcaaggagc tcgcgcgcat tcacgtcgag aacaaagcgc gcgacaaggc gggcgaggtc
     4321 tatcgcaaga tcctcgagat cgtgccgaac gatccggacg cgctgcagta cctcggtcct
     4381 caggccgccg ccgtcgcgcc gcagccgccg gctccgacgc caccgccgat tcccgcgccg
     4441 ccaacgcgca acacaccacc tcctccgccg ctccgcagcg gtacgccgat tcccgccgcg
     4501 gttcgccagg caaccgccgg cgcgaagttc aacatcacga ccgatctgcc cgcgctagac
     4561 gtcccgccgt cgtcgcgcat gaccggtgcg atgcctctcg tcgacgagca gagcctttcg
     4621 ggtgtcgact tcgcgctgcc cgaatacgac gacgccgatt tctcggcgga catggaacca
     4681 gcgccggatc cgcgtcgcgc gtctgccgcc ggcgagcgtc acgccgagga gatctcgaag
     4741 atcctcgccg agaccgacgt ctacgtgaag tacggcttgc atcagaaggc cgtcgatcac
     4801 ctgcgtcgcg tgttctcgct cgattccgaa aacgtcgaag cgcacgagcg cctcaaggac
     4861 atcttcatct cgcaaggccg cgagcaagaa gccgaggtcg agctgctcaa gctcgccgag
     4921 ctgatcgcgc cgagcgatcc cgatcgcgcc gaggcgtacc tgcaagagct cctcgcgatg
     4981 aacggcacgc acaccggtgc gttcgagctc gcacgcaagt tccagctgcg cgtcgcgcgc
     5041 atgtcgagcg tctccgccga ggtcgagtac tcgggtggcg gcgtcgccga ggtcgatccc
     5101 gagctcgatg atctcgatct ctcgagcgcg ccaccgtcgg cgatgggcat cgcgcgcaac
     5161 cagatggcac atggcagccc ggcgccggcg cgtccaccgc gtccgccgaa gggcttcgat
     5221 gatggcagct tcgacgtcga gctcgactcg caggtcgacg ccgtcaacga gtactcgtcg
     5281 caggccgcgc actcgacgcg tcaggttcct gccgacgaca tgaacgcgct cgcgcagcaa
     5341 gctggctacg acgacgaaga cagcgccgtg cttgtcgatc gtccgcccgc cggtggctgg
     5401 aacgagcagc agtatccacc gacgacgtac gaaggtgcgc aggcctacgg ccactcgctc
     5461 gacgacgccg tcgatcgcca gctcgacgac gaagcactcg gacacgacat cgaccagggc
     5521 gtcgcagccg agctcggtac gcgcgagagc gtgtccgaca tgccgttcga tccgcacgac
     5581 gcgcgcgaat tcgatcgcgg cgtcagcagc gcaaaagacg gcaccgacgc ggtctacgcg
     5641 aacggctacg ccgagacgga gctcgcgaac ccgagcgcga ctgcgtcgta cgacgacgtc
     5701 agcgacccgc tgtccgtgtc gggcagctac gatccctacg gcaccgacaa ccagcagccg
     5761 agctacgaag cgacggtgct tggtgggccg atcgtcgacg acgtcgacac gccgcccgtc
     5821 ggtggccgcg ccgaatcgct cgtcgaggac gacctcgaag aggtcaactt ctactccgat
     5881 cagggcatgt accccgaggc gatcgactcg ctgcgcggcc tgttcgatcg ctatccgaat
     5941 cacccgctgc tgatggcgaa gctgcgcgag ctcgagtcgc tcgagcaggg catcgagccc
     6001 gcgattcaca cgccgcccgc cggtcagcat tcgggagccg acgtgagcac cggtaccgac
     6061 gcactcgatc tcgacgagat cgaggaggtg tcggccgacg acatgatcga ggaggacggc
     6121 gagccgcgcc cggctggcaa gcgcaagccg tccgtcatgc tcgagaagcc cgtcgacgag
     6181 agcgacgccg acacgcacta cgacctcggt ctcgcctaca aggaaatggg cctgcacgac
     6241 gaggcgatca aggcgttcga gaaggtgctg cgctcgcccg gtcgcgaagt ccagtgccgc
     6301 gtgatgatcg gcatgtgtca tcgcgagacg aacaacccga acgaagcgat ccacgagttc
     6361 aagcagggcc tgcacgcgaa cccgagcgat cgcgagcgcc tgagcctcta ctacgagatc
     6421 ggtgtcacct acgaagcaat gggtgacctc ggcgaggcgc tctactacta cgagggcgtg
     6481 ctcaagcgcg acccgaactt cgcggactcc gcgcagcgcg ccgacgcgat ccgtatccgc
     6541 ggcacgcgtc cgagccagcc tgccgacgac gagatctagt ccgcgttggg gctcggccaa
     6601 ggccctgcgg cctcaaccta agcgccgcgc tgaccgagcg caacgcacga tcgcgcccgg
     6661 gcggctcacc gtcagctgct cgccccagcc ccagatggaa cgccaagctg ccaaggccgc
     6721 caagaacgcc aagaagaaga tctacaggga tcggacaaca ctccggctgt gagggatcga
     6781 gggcgcggtt ctggctggtc gcttgctcgt gacgcggtcc agaaatcgga ccgtggagtc
     6841 gaccagcaag ggaatggccg cccttgacga ttcttacctt ggcgttcttg gcgcgcttgg
     6901 catcttggcg ttcgatcttg gtcccggctt tcggcggcct gacgcatggg tagctgaccg
     6961 aactggacga tcaacgcacg cgccgccgcg tgcgctccgg accatgatgt cggcgtgatc
     7021 gtcgacgtcg cggccgagga cgaggagcta ctcgagcggt actaccgcga gctctacctg
     7081 cccgagttcg cagcacagcg cgagccgctc gaagcgtggc agagcgcgct ggccggcgac
     7141 cagccgtacc agatgttcgt ccgcgtggtc gtcgagcgcg gcgagatcct cggtggcatc
     7201 agctacgagc tgtatccgca gagcgggtgc ggcctcgtca cgtacatggt gatcgcgcct
     7261 gctgcgcggc gcagcggctg gggcaagcgc ctcctgttcg aggccaccaa ggagctgatc
     7321 gcgcgtggtg cgctggcggt gctcggcgaa gtgaatgacc cgcgcggcac cggcgaatgg
     7381 gaccgactcg agcggtttca acgctggggc tgtcgcgtgc tcgacgcgcg ctacgtacag
     7441 cccgcactcg ggcctgggct cgtacgcgat cgcaatctag tgctcatcgc gttaccaccg
     7501 attcgcgagt cgattgccgg tgatgtcgtg cgcacgttca tcgccgagct gcacgaggtg
     7561 accgagcgct gctcgcccga cgacgagatg cgcacggttc tcgacgagat tcccgagcgt
     7621 gttcggctcg tcgagctgct gcgctagacc gcgtcgttcg tggtaagccc aaggcatggg
     7681 tacgcggacg gagagtgaca gcttcgggcc gatcgaggtc gacgacgagc attactgggg
     7741 cgcgcagacc cagcgctcgc tcgagcactt cgcgatcggt gaggagaaga tgccgatcgc
     7801 ggtcgtgcac gcgctcgcgc agatcaagaa ggcggcggcg ctcgtgaatc gcgacctggg
     7861 cctgttatcg gacgagctca caaagctgat cgtgcaggcg gccgaggagg tgatcgaggg
     7921 tgagctcgac gacgagtttc cactttcggt gtggcagacc ggcagcggca cgcagacgaa
     7981 catgaacgtc aacgaagtga tcgcgggccg cgcgaacgag ctatcgggtg gcaagcgcgg
     8041 cggcaagaag ccggtgcatc cgaacgatca cgtcaacctg tcgcagtcat cgaacgacgc
     8101 gtttccgacg gcgatgcatc tggcggcggt tgccaccgtc gaggaattgc tgttgcctgc
     8161 agtcaagcag ctgcgcggca cgctcgacgt caaggcgcac gcattcgccg acatcatcaa
     8221 gatcggccgc acgcacctgc aggacgcgac gccgctgacg ctcggccagg aaatgtcggg
     8281 ctgggtcgcg cagctcgacg ccgggcttgc cgcgattcgc gcagcgctgc cagccgtgca
     8341 cgagctcgcg ctcggcggta cggcagtcgg caccgggctc aacacgcacc ccgagtatgc
     8401 gaaccgcgtc gcgcaggcga tcgcgctcag tacgggcagg ccgtttgtca ccgcgccgaa
     8461 caagttccag gcactcgcgt cacacgatgc actgctgaac ctgcatggtg cgatcaagac
     8521 gctcgcggcg agcttgttca agatcgcaaa cgacatccgc tggctcgcga gcggaccgcg
     8581 ttccggcatc ggcgagatct cgatcccgga gaacgagccg ggcagctcga tcatgccggg
     8641 caaggtcaat ccgacgcagt gcgaagcgat gacgatggtc tgttcgcagg tcttcggtaa
     8701 cgacgtcgcg gtcaccgtcg gtggtgcgag cggcaacttc gagctcaacg tgttcaagcc
     8761 gctgatcgcg cacgcggtct tgcaatcgat ccgcctgctc ggcgatgcgt gcgagtcatt
     8821 cgacgagcat ctcgcacgcg gtatcgagcc gaatcgcgcc gaaatcacgc gcaaggtcgg
     8881 taacagcttg atgctggtga ccgcgctcgc gccgcatatc ggctacgaca acgcggccaa
     8941 ggtcgcgaag aaggcacaca aggacggcac cacgctgaag gaagccgcga tcgagctcgg
     9001 cctcgtcagc gccgaggact tcgatcgctg ggtcaaggcg gaggcgatga tcggaccggc
     9061 ggctcgagag taggcggcct cttgcgcggc tcgccgatct tgatcgagag gttggtcgct
     9121 gtcacgcgat gaacgacgac ggtcaccgcg gtcggccgga agccgtcgaa cgtgacttcg
     9181 agctgctcga cgccgtcgaa gtgcagtcga tcggccgata gcaccatcgg cccgatcttg
     9241 agatctttga tcgtggtgtc gacgctgccg atcgcgccct tgagcgcacc cgcggtgcga
     9301 tcgtggatgg cgcccgagac cttcagatcg accatgccgg gctgatcttc gccgccgaac
     9361 atgatcatgc cggcgccgac gtcgaggtga cgcggcgcga gtaacgacaa cgcgacttcg
     9421 atcttgtcga gcgcgaacgc cttgagcacg ccgcgcttgc gcccgatctc cgactccgtc
     9481 ggcggcttgt tgctgctcgg caggcggaag tcggcaagcg agcgcaccgg cacacggccg
     9541 aacgacgcga gcgtggcagc gtcggtgtcg agcgcccacg tgatcagatc gtggccggaa
     9601 ccgaagatcg gcaccttcca ctgcaccttg agtcgctgat cctcgaactt gagatcgagg
     9661 aacgtgcctt cgagccagtc gaggctgtct tcgagcgcgc gaaagtcgag gctgccttct
     9721 tgaatcggca cgcgcagctg ctgatcgagc gtgcgcacgc cgagcaccgg cagatcgagc
     9781 tgcaccttga gcgtcgtgta gatgcggccc gaaagactgt cgaggaaccg cagcttctct
     9841 tgccgctgaa ccggcgagct cggttgcggc gcgggtgcgg gttgtgccgc gggcgccttg
     9901 cgaccgaacg ggcctttgac ggtcagccgc agctcggaga acgtgacgtg gggcgcgagc
     9961 agctcgatgt tcgcatccgc cgcacgcacg agccgaaggc catgcgggag ctcggcgcgc
    10021 tcgacctgca gatcgtaggt gccggcgtcg ttgcgaagcc acgcgctgcc gaccgacgcc
    10081 gactccgctt gccagcgcag cgtatcgagg cggccttcga ggccctcgaa cgtgcagtcg
    10141 gccgcgccat acgtggtttg cccggcgcgc ccttcgatct tgtgggtctg cgcgcgcgga
    10201 aagaccaatc gccgtccttg cccttcattc gaaaaaccga agcgctggac ttcaatgtcg
    10261 gcgtcgacct tggccataca acgtcggcac tttagcgcga cttagccgca ctcccgaggc
    10321 gccaccgatc caatcgcaca cgtcggtcgt agagcccaca gcaacaacca ggggagggcc
    10381 agatggccaa tcgagtcctg gtgacatcgc tggcagcgcc gctcgcgctg acgggatgcc
    10441 actcgtctga tgagctacct actgatccgc cgaagaccgt cgaaaaaatc gcatcggagg
    10501 gctt
//
LOCUS       DASUFX010000717         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_113713,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000717 DASUFX010000000
VERSION     DASUFX010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113713"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFV99_28905"
     CDS             complement(<1..533)
                     /locus_tag="VFV99_28905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063444.1"
                     /translation="MSEAFPERGLALHMPATPSPCADPCAVVDVNDGGASYAAALADA
                     LRKKRGTELPDEIAEAQESLERRIRAHLAKGRVAVTLTDNRYTMISVRRLAKERRYEV
                     RLHHMFADADPVITRALARYIAENDPEASRVLGDFIDQNSGHVRGRARRQPTQVIFTA
                     GEHHDLRQIYDDLNARY"
     gene            808..2610
                     /gene="aspS"
                     /locus_tag="VFV99_28910"
     CDS             808..2610
                     /gene="aspS"
                     /locus_tag="VFV99_28910"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5063445.1"
                     /translation="MSEFLATHTRTHSCGALRADDVGKHVVLTGWVKTYRDHGNCVFI
                     DLRDRDGITQLVFDPSFDKAAHEVARDLRSEWCVGIVGEVRSRGGNVNDKLPTGAIEV
                     WVKALEVFSKAETPPFPIEDQIDTNDQVRLKYRYLDLRRPKLQRNMIMRSAITRVTRD
                     YLGKHGFLEIETPFMVKYTPGGARNFLVPSRLNPGSFYALAESPQIFKQLLMVAGYER
                     YFQIVRCFRDEDLRLDRQPEFTQIDLEMSFVNEQILQTTMEGLITRLWKEVLGVEIKL
                     PMRRMPYSEAMDKYGVDKPDLRCDLVLCDVTEAAKNCGFRVFESVVGNGGIVKCLRVP
                     GPPDKLPRSILDGLNDFAKSFNAKAAWVRIQEGHQWQGLKGFSDEARAKINHIAGAEV
                     GDTLIFVADKAKVANATLGAIRIHYIGEKLGLIRKGEWQFMWLTDPPLFEIEDGGAAG
                     PVLAAAHHPFTSPRVQDEGLLETAPEKVLARAYDLVLNGVEVGGGSIRIHRSELQDRA
                     FKALGISEEARREKFGFLLDAFKYGPPPHGGIAFGLDRLAMLMTGAESLRDVIAFPKT
                     QKGSDLMTDCPTGVAKKQLDELFIQLKPDLPVKT"
     gene            complement(2667..>3161)
                     /locus_tag="VFV99_28915"
     CDS             complement(2667..>3161)
                     /locus_tag="VFV99_28915"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5063446.1"
                     /translation="RLIDSVIQHTAPLNPGNSGGPLLDTSGRVIGVNTAIIARSQSIG
                     FAIAVETAGWVLSQLLAHGKVRRAWLGVGAARRPLDRRLAYHHGLGASAVEIQSVEPN
                     SPAAKTGLVDGDLLVRFADIAIETVDQLHKSLRTWQAGTPVKLGVIRRGVRIDLEITP
                     AWSA"
BASE COUNT          566 a         1014 c         1033 g          548 t
ORIGIN      
        1 aagtaccggg cgttgaggtc gtcgtagatc tgccggagat cgtggtgctc gcccgctgtg
       61 aagatgactt gggtcggctg gcggcgcgcg cggccacgca cgtgacccga gttctgatcg
      121 atgaagtcac cgagcacgcg cgacgcttcc ggatcgttct ccgcgatgta acgcgcgagc
      181 gcccgtgtga tcaccgggtc cgcgtccgcg aacatgtgat gcaggcggac ctcatagcgc
      241 cgctctttcg cgagccggcg taccgaaatc atcgtgtacc gattatcggt cagtgtgacg
      301 gcgactcggc ccttcgcgag gtgagcgcga atcctgcgct cgagactctc ctgcgcttcg
      361 gcgatttcgt ccggcagctc ggtgccgcgt ttcttgcgaa gagcatctgc gagtgccgcc
      421 gcgtagctgg cgccgccgtc gttgacatca acaacagcac acggatcggc gcacgggctt
      481 ggagtggcgg gcatatgcaa cgcgagccca cgttcgggga acgcttcact caactggata
      541 actcctctta tttaagcaga catcgaacgt tgggcatacg acggcctttc gggcgcaggc
      601 cccccattac gggaggagcc ttatgggcgc ccgattcgct cgctaacccc gccgcctacg
      661 acgagaaaaa tgctatcgcg ccggtttgcg atggatcaaa ttcctgactg atcgagggat
      721 cggatctgat cgcaactgtt gggaaaaacg catgtaggcg gggatcgcac cgatctgggc
      781 gaaaccgggt agagtcccct ccttcctatg agtgagttcc tcgccacgca cacccgtacg
      841 catagctgcg gcgcactccg cgcagatgac gtcggtaagc acgtcgtgct gaccggctgg
      901 gtcaagacct atcgcgacca cggcaattgc gtgttcatcg accttcgcga tcgcgacggc
      961 atcactcagc tcgtgttcga tccgtcgttc gacaaggccg cgcacgaggt cgcgcgtgat
     1021 cttcgcagcg agtggtgcgt cggaatcgtc ggcgaggttc gctcgcgcgg cggcaacgtc
     1081 aacgacaagc tgccgaccgg cgcgatcgaa gtgtgggtca aagcgctcga ggtgttctcg
     1141 aaggccgaga cgccgccgtt tccgatcgag gatcagatcg acaccaacga tcaggtgcgc
     1201 ctcaagtacc gctacctcga tctccggcgg ccgaagcttc agcgcaacat gatcatgcgc
     1261 tcggcgatca cgcgcgtgac gcgcgactac ctcggcaagc acggcttcct cgagatcgag
     1321 acgccgttca tggtgaagta cacgccgggc ggcgcacgta acttcctcgt gccgtcgcgc
     1381 ctcaacccgg gctcgttcta cgcgctcgcc gagtcgccgc agatcttcaa gcagctgctg
     1441 atggtcgcgg gctacgagcg ctacttccag atcgtgcgct gcttccgcga cgaggacctg
     1501 cgtctcgatc gccagcccga gttcacgcag atcgacctcg agatgtcgtt cgtcaacgag
     1561 cagatcctgc agaccacgat ggagggcctg atcacccgcc tgtggaagga agtgctcggc
     1621 gtcgagatca aactgccgat gcggcgcatg ccgtactcgg aggcgatgga caagtacggc
     1681 gtcgacaagc cggatctgcg ctgcgacctc gtgctgtgcg acgtcaccga ggcggcgaag
     1741 aactgcggct tccgcgtgtt cgagtcggtc gtcggaaacg gcggcatcgt caagtgcctg
     1801 cgcgtgcccg gtccgcccga caagctgccg cgctcgatcc tcgacggcct caacgacttc
     1861 gcgaaatcgt tcaacgcgaa ggcggcatgg gtgcgcatcc aggaaggcca ccagtggcag
     1921 ggcctcaagg gcttcagcga cgaggcgcgc gcgaagatca accacatcgc cggcgcggag
     1981 gtcggcgaca cgctgatctt cgtcgcggac aaggcgaagg tcgcgaacgc gacgctgggc
     2041 gcgattcgca tccactacat cggcgagaag ctcggtctca tccgcaaggg cgagtggcag
     2101 ttcatgtggc tcacggatcc gccgctgttc gagatcgagg acgggggtgc agcgggtcct
     2161 gtgctcgcgg ctgcgcacca cccgttcacg tcgccgcgcg tgcaggacga agggctgctc
     2221 gagaccgcac ccgagaaggt gctcgcgcgc gcctatgacc tcgtgctcaa cggcgtcgag
     2281 gtcggcggtg gctcgattcg tatccatcgc agcgagctgc aggaccgcgc gttcaaggcg
     2341 ctcggcatca gcgaggaagc gcggcgcgag aagttcggct tcttgctcga cgcgttcaag
     2401 tacggaccgc cgccgcacgg tggcatcgcg ttcggtctcg atcgcctggc aatgctgatg
     2461 acgggcgcgg agtcgctgcg cgatgtgatc gcgttcccga aaacgcaaaa gggcagcgat
     2521 ctgatgaccg actgcccgac cggcgtcgcg aagaagcagc tcgacgagct gttcatccag
     2581 ctcaagcccg acctgcccgt gaagacctag cgagctgcgg ctacgcgttc caccagctca
     2641 cctgctcgag cacgcgcgac ggtagctcac gccgaccagg cgggcgtgat ctccaggtcg
     2701 attcgcacgc cacgtcgaat cacgccgagc ttcaccggtg tgcccgcctg ccacgtgcgc
     2761 agactcttgt gcaactgatc gacggtctcg atcgcgatgt cggcgaacct cacgagcaag
     2821 tcaccgtcga cgaggccggt cttcgcggcc gggctgttcg gctcgaccga ttggatctcg
     2881 accgcggatg cgccgagccc gtggtggtac gcgagcctgc gatcgagtgg acgtcgcgcc
     2941 gcgccgacgc cgagccacgc gcggcgaacc ttgccgtgcg cgagtaactg cgagagcacc
     3001 cagcccgcag tctcgacggc gatcgcaaag ccgatgctct gcgagcgcgc gatgatcgcg
     3061 gtgttgacgc cgatgacgcg cccactcgtg tcgagcagcg gtccgccgga attgccggga
     3121 ttgagcggcg cggtgtgctg gatgacgctg tcgatcaggc g
//
LOCUS       DASUFX010000718        24122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_255394,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000718 DASUFX010000000
VERSION     DASUFX010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255394"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VFV99_28920"
     CDS             complement(<1..1980)
                     /locus_tag="VFV99_28920"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063447.1"
                     /translation="MGAEPYTDVATHVRDELRRVWLRLEYQIRLGWRKESGLAGEDVI
                     GPEDMGRLFSIARGAPQVTGDSGADQVLEQWLAAHETTEARIRATIEARTRSPLVDLI
                     RTFNLTARQWSSLVFALMPEVDPNLVQGYRYLARDPNVRGLDGRLLAQFVYDTPQTRS
                     MMARDLSASSPLLRYRLLDLSGGTGQSDSLMFRRIRAATRLVYLLDGGALELDPELAE
                     VAELRFGPVSGAFPAVTVDRATSAMRSNEVVLAVQGQRGVGKKLLLQLAAAHWNQRLL
                     LIDAKRLSQLQLAAQQTMVRSLVRELMLLDALPAFIDVDDAAVVDADRDAMPAFFDMF
                     LDEWSGPVAMTINRERMPRIHQRPLVHLTIEVPPLQVRAAMWQTAVPSLSAQDAEGLA
                     GRFAIPGGIVVAAAQAAEAGRMPESGPPSAVDLDQAVAAQLHQRISRLGKKLPTPFDL
                     DDLVVDDDTRAALVEIAAAARERRKIRDQFKLRGAQGISVLFSGHPGVGKTMSGTVLA
                     KRLGLDIYEIDLSQVVSKWLGETEKNLSDVFDAAEPGHVVLLFNEADSLFGKRTSDVK
                     SSNDRYANLETNYLLQRLERFNGLTILTTNLTGAIDQAFKRRFTYDVYFTFPSPDMRA
                     ELWRRTLPERARSADIDFDALAEKFELTGGFIKVAC"
     gene            complement(1993..2649)
                     /locus_tag="VFV99_28925"
     CDS             complement(1993..2649)
                     /locus_tag="VFV99_28925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063448.1"
                     /translation="MRVALLALLIAASCGRQPPPRVSTGSGSASESNGSNGGTNMNGA
                     VTVTLVGQKAGRPPTEHLLVDVQLKNDDQTPRWVLIPSVLPVDSGGGVDKLEQLTVNA
                     GATTLAVGRFLGRAGTYALKLGPGARVTLRKLDVSWWREDPTKQKEAAFDIRLANDVK
                     LGDQTMATWFDKDPAINGAADVDMEAAKHTRSFKSPTGDEVEVLTVQAMAVPVKLAAP
                     "
     gene            complement(2668..3861)
                     /locus_tag="VFV99_28930"
     CDS             complement(2668..3861)
                     /locus_tag="VFV99_28930"
                     /inference="COORDINATES: protein motif:HMM:NF039793.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063449.1"
                     /translation="MAREHDTRRAQPADAGTTDAAHSETTSGTTFTGGHAPAVDTTAS
                     TASASASDRAFAAQPQAGGPEIPATKEAMGGVVVKDMQKANVEPGSGGQNDVNHGIHY
                     WYNYQRQCENAGQPELWQDKYRSGHTQAQAFVNPHESGRWMDWELKKGHSASKAIKEW
                     LAGATVAECLSSVIAMEIDALRAAIGDKKFDKMFGSADPKEDKAIPAQQRMHVKAGIS
                     GTPVANFMKSTEVADEAYKNGGQFGSVEPAKLDKELVPGQWYYFYNHPKYLLKHPGGA
                     WQGENALFMGKNDAGERLWSGLGATATEDAMVDEMVRAYNAGRNDYDERIMKEQGSMD
                     ASGKFTDPLYDPKSGKFPDKVTKKEILESQPYSLGGTERKGGFMATAGMELDEKRVQK
                     TRDQE"
     gene            complement(3938..5674)
                     /locus_tag="VFV99_28935"
     CDS             complement(3938..5674)
                     /locus_tag="VFV99_28935"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5063450.1"
                     /translation="MGQVVRLVCALLIALLGTAHAEPPQQQKIALTAKPSVVRVWGAY
                     VADYEIAGQKIPNCCAVGGSGTGFFITADGYIATNAHVVSDVADGEVVAKGKLYQQML
                     SEVRRALGPEMARMSDRQIRDLLSEIKLVNLETRAYVVLPSGEKLDYEVKQYGKPGTG
                     RDAAIIKVKIDNAPALPIGDSTRSQVADQIVVIGYPGVADLRELLDEKSQLQATVTEG
                     TVSSLKHATSGEQILQISAPITHGNSGGPALDMKGNVIGLATFGTDVQGFNFLVASAT
                     LQEFVKKEKLDPKPSKTLAEWQKGLEHYWADEYTAAIENFRDVETLFPMHSEAGTLIR
                     QSNEAKKEGKEKKPSGGNGGLIAGLVIGGLVVVGGGGLMLAKRGKKAAPSPQQAMPGA
                     PHMPPGAPHMPPPGAPHMQPPHVQQGYPPPPQFAPPQQQMYGRVQPQNAPPMMPVPQQ
                     PIAKTVAISGPNQSGGIAPTAFGSLSIGSLWCTRGSLQGQRFALTATGIIIGRQPGVA
                     HVLVNDHRASGKHVWIGIDQGKLVAIDQGTTNGTYINDVMRGRITRAELRDGDVVIVS
                     EPDCLSLQIRLG"
     gene            5706..6890
                     /locus_tag="VFV99_28940"
     CDS             5706..6890
                     /locus_tag="VFV99_28940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063451.1"
                     /translation="MGFGISRHARSRKGRGLFRKAPDPKEVGDRLGRLARRMLRDAVV
                     RSGAKGDRGEYRYIVELKMHPVAPAGTLSVQPDGEIVLKVETSTLGPGYHAHVIDKVT
                     PILEELDYVWTDTFDPAATQAAMCAWLADELRGNDKVTIGVPDTRRFRVDAPVLTALG
                     ARDAAWRDAVLADPAKAADAFAWWQTGPGRDVLARALITMSLEVPWREPLDKGERELM
                     TEVDEDLRAARKADPALPLPYPEWKELLMHLGIEDEDVNEKVGDRTSTLGYRRHDIEI
                     ELSGGWLVVIPGAFAGHWEDDGAKYWATDGDRMIEFSSLTANDETDSAKLLAVAPEKY
                     EVVERLVDGNHHGRAETFEENDVPILVGLMCNAPHVGILTCKGGTREWALATWRSLQQ
                     KN"
     gene            complement(6971..9145)
                     /locus_tag="VFV99_28945"
     CDS             complement(6971..9145)
                     /locus_tag="VFV99_28945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5063452.1"
                     /translation="MGHLRLLVVLGLVACKPPYLHGGGDDGTGPDAPADDSTPLRSCT
                     SSITYHGDAGVTAVDIVGAWDWSARTALADPDHDGTFTGELELAPGIWPYKLVVGYDG
                     GGVDWVLDPQNPYRAYDGGVENSALRVADCAPPLLEVISHDTNTTRVRVSRGKSAAAI
                     TDIAVTLQGAPIDFTRSGGELTIATPGLAAGKYTLRITAKDAAGASAEPVLVPFWIEA
                     DPFDWRDALIYMVMTDRFRDGDPTNNPAPSAGADPGAEYKGGDLRGVASAIEDGTFDA
                     LGVRALWLSPFVANTTRVEYENGHGVTAFHGYWPVKARAIDPRLGSEADLDALVAAAH
                     KHGIRVLMDFVINHVHEQHEYYAAHPDWFRTGCECGTPGCDWTEHRLDCSFHPYMPDV
                     NWQNKDAGEQMIADAEWWLERFDLDGLRVDAVKHVEDLAISNLSTRIHERFETGGTEY
                     YLLGETAMGWGGDDINNSLNDYATIARYIGPFALSGQFDFVLYHATTTNVFADEARGM
                     LHLDYWTKASLDHYPADAVMSTYVGSHDTERLVSLADLGSGSTIVHHKWASDGLPAMP
                     TTDLPYDRAAFALQWLLTTPGAPLLYYGDEYGEHGGADPDNRHMWAAPSARTPRQRAM
                     FDKVARAGKLRQTLEPLRRGAYVQLNSTDDVLAFARVTASEAAIIVANHAATARTLTV
                     HSAPIGNGTLTDRLDPGSRTVDVSNGSFTITMPPRSVSVLTH"
     gene            complement(9205..11136)
                     /locus_tag="VFV99_28950"
     CDS             complement(9205..11136)
                     /locus_tag="VFV99_28950"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5063453.1"
                     /translation="MRRLAALAVLAFLAGQAIADDGDDDAFDKPTAIRITHGGATARI
                     QARFAFDVDGPGVELNAHTFAIPRNGVVTAATVTVDGTRHALRLENAEAADKAFEALT
                     LKPGTGSDRSWAFVIDGGNDQVTLDLMAPHNAHVVLDLALEVGTCFVKDARFVELPES
                     WWQRVPAKQRTAIPTDGVGEICDDSGDETSHWIGVASRDLMKQSPGEHRIGTIAGRLP
                     LDSTHFARVEIDVASVLTDVPADLHTAIVIDHSRSLSTDELEAQRATALAYVRGAPMS
                     HVQVIGYARTAEPLLPSWMVASHAAPQIDRAIRALPPRNGSNVDAALGEAVAWLSRVQ
                     GTKRIVLFSDERLAERFGESAALRALVPDGVLVHVVHPTTGGSITRFDDEESVLATLA
                     KATEGIAVFAGVDDGATPDATMLLRPISIDHLAVSATGWKTVDHFADMHECGDSLAEG
                     MTCTWWGEGDGAAGPITIAGLLWNKPIKRVVRADPTQARTLARALSVMHVLDVDLQEQ
                     VDQVALAINSVWSLFGEWGGDGGYEDIGGLGTFGFGRIGTTSHDTGIGTSIGHLLQRL
                     DLSAQLKQAITSCNPGTARGGIDVETTLEEIVAVEVTVTPANPTLHDCLVEAVWNTTL
                     GIGNAPQSARTHVAFGNKR"
     gene            11192..11890
                     /locus_tag="VFV99_28955"
     CDS             11192..11890
                     /locus_tag="VFV99_28955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063454.1"
                     /translation="MGNKFGMSPFDILAAFGPVLVLGPVVLWMLLFGQLLIYPIARWK
                     AQREAVMDPQLGIKFALHFFALLAFQIVLFALATALYTLFSKAEDKGELYRMAFGFLI
                     PGGGVLAAHYVLLKRTNQDQFPGVRRLFLGYNLLVTGLIGFVMLLVAFQVFFRRGPAG
                     NDGRFAIASVLVYVGAWVACGLHFGKLVLGDSAAPPSNVIPPSVPTHAAAQQSGPVLP
                     SLSAGTFPPIDPKK"
     gene            complement(11850..12881)
                     /locus_tag="VFV99_28960"
     CDS             complement(11850..12881)
                     /locus_tag="VFV99_28960"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5063455.1"
                     /translation="MSYKLRLTLITIGVLATLTLAHKKPWRGSATASAAELQVGFLPV
                     TCHLTCPVTDFATKTTKTGTRFLSQRFTEFPTVVEALKAKKIQATFLLAPLAMVLREQ
                     GVPIHIVYLGHRDGSTVIVPKNSTAKSIRDLKGKTFARPSRFSNQYLVITKLMEDEGM
                     QPGDINFVDLPPPDMPSALAAGAIDAYFIGEPHAAKAELDGTGRVLYYAKDIWPHFVS
                     CVLVVRDELINEHPEIVRDLVRGIAESGEWAEQHRLEAAKVAAPYFRQDQKLLEFVLT
                     QPPDRVSYRQLTPSDEDMTQIMKYAVKAGILKKPLDVSDIVDRRFIPKEIHPAAITSS
                     DRSAETSPH"
     gene            complement(12878..13561)
                     /locus_tag="VFV99_28965"
     CDS             complement(12878..13561)
                     /locus_tag="VFV99_28965"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5063456.1"
                     /translation="MSVVLELNGVGFDYGALSVIDNLDLTVSKGELVVCVGPSGCGKS
                     TLLSLLGGHLQPTRGTIVRKGASRTIYQDGGLLPWLTVRQNIALGNPAKVDEWLRITR
                     LADFADAYAHQLSGGMRQRVEISRALAAETDILLMDEPFSALDYQTRRRMRMELVTTL
                     AERPRTVVFVTHDIEEAVQIGDRVIVLGTRPANVRKTLELPPGRSRLPAAPGVAEAEQ
                     AIMEELGLS"
     gene            complement(13558..14340)
                     /locus_tag="VFV99_28970"
     CDS             complement(13558..14340)
                     /locus_tag="VFV99_28970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5063457.1"
                     /translation="MRVPAKGRSYISNIGFPIITGLVILAAWGAASAADMFPTGTVPS
                     PEAVGHAFRAELASGRLLDDVIASLYRVAWGFLTAVVAAVPLGLVIGRHAAPRAALMP
                     WVNFFRSLSPIAWIPFAIIWFGVGDPPAIFIIFLATFFQVVLATAAASASVPKVYYRV
                     ADDIGLRGMQVLFHITLPAIMPQLVIALRVAIGVAWMVVVAAEMIAVRSGLGFLIVDA
                     RNGLRMDLVVCGMITIGAIGIGLDLAFSRLAKIPSLRWGFER"
     gene            14414..15064
                     /locus_tag="VFV99_28975"
     CDS             14414..15064
                     /locus_tag="VFV99_28975"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5063458.1"
                     /translation="MKKVAVLGSGQVGDALSNGFLAHGYAVMRATREPKKLESWKTSA
                     KGEAAIGSYDEAAKWADTIVLAVKGLIADQVLDQCGLANLAGKTIIDTTNPIGSEPPD
                     NGVIRYFTNANESLMERLQKKVPQANFVKAFNSVGNSLMVNPKLDTQPTMFICGNNAG
                     AKQEVTELLTKFGWETADMGGVEGARPIESLCILWCIPGFIRKEWMHAFKLVKARG"
     gene            complement(15079..15870)
                     /locus_tag="VFV99_28980"
     CDS             complement(15079..15870)
                     /locus_tag="VFV99_28980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063459.1"
                     /translation="MTVAGVLDVSADDSTDSGPGGDYMTSGGAPSNTNGAGGGGAGFK
                     TNGGAGGDSNTNGGANNGGAIVDAAAAMGLLPGASGGGSTSSRGGYGGGGVTLVSCRG
                     TVSVTGIIDAGGGGGAGGNSGSSPYGGAGGGAGGNVVLQGRMVTVTGQVFANGGAGGS
                     GKPFTTVNSVGVAGTDGMATTTCALGGTATDASPGGDGGCAQTSPTNGIKFRLTPAPP
                     SAPSGGGGGGSVGYFRAFTPAGVSPTLTPAAASPTFEAPGTVDTH"
     gene            complement(16512..17894)
                     /locus_tag="VFV99_28985"
     CDS             complement(16512..17894)
                     /locus_tag="VFV99_28985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063460.1"
                     /translation="MRLKSFALVLGLVAVVGCGDDPDQSLNGVFPSSGFIGRKVRVEV
                     SADNVAFEDGKVSLDFGSGVTVSAVTVSSPTALFADITIADTAPLGTHDVVVHNGNDT
                     LTMKEAFKLESPVKVDFKGTLAQGSVVTFTATNLDLANLYDATCGASIFGICLLYTNM
                     QVNTPAGVTAVVDTVEPFRVSGTLFVDVDAMAGDISFVSGPADVADQQVTSAMGTPTD
                     FPARAPVALAAGTATTTTVMAAFDSHLYSFDAAATSVSRYSVSPSDPNATPGVYILPA
                     SGHFADAVAASSKPNVIVETAGKYFTVYTDASGSSGYSYAIRVNPQTLVAAAEADTAG
                     ANDTFAAAQNLATNSTILLTNASISDDLDEDWIKFTVPANSTTKKVHVVTAGDPMTDT
                     FVEIWKGDPTVTANKLGVGTDSGYHEDVLTTSTVGAATTIWVHIQWSPDFIDTYSTAH
                     SQYVAAIWLE"
     gene            18360..19061
                     /locus_tag="VFV99_28990"
     CDS             18360..19061
                     /locus_tag="VFV99_28990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063461.1"
                     /translation="MKGLLSAIVMMVPVVAFADEPAPNTGTPEATAAPTPSGQLPPAE
                     SDKDPAPPVPTPNIPGLNGDIVEQAGVGGTVGYGRAGVLELGGAAGLTLAQDIRAVNF
                     SPSIGWFLVDNLELSAILDVTNLKAGGESATLWSALAEPSFHLPINRSLFGFVGMGVG
                     AAYVSQLGTGLAVAPRIGMDVMVGRSGVLRPSLAYEYTTHDAMGSVDANGNTDVTLVA
                     ISSALRFNIGYSTMW"
     gene            complement(19130..19765)
                     /locus_tag="VFV99_28995"
     CDS             complement(19130..19765)
                     /locus_tag="VFV99_28995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063462.1"
                     /translation="MVKLAARTLVALGLVALTPVAAAHPPPPDDYVESEPAPVVDTDP
                     AFNMFGFRMSAGALPLDGGRTTALSIGLGVEHPVFKKTRVFGEYEWLWLTWMDDRALT
                     SMAPRPERHGTGHRASLGLRRELLGKNLGRSVRVFIDGELGATFALVDDNMRGVSFVP
                     GGLGGLRFGYDIYSRSDDSPSRTFEIELLARAVAIEGGVGGMMGLGMAWGN"
     gene            complement(19759..20355)
                     /locus_tag="VFV99_29000"
     CDS             complement(19759..20355)
                     /locus_tag="VFV99_29000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063463.1"
                     /translation="MTIVALAGVARADDDDTDEDGAFMPVSKRSIALAFGGHGTRIGG
                     LSEGGVGPTLELALGSGRWQYFTEGGFATSNVSTSTANPGTSVSGRMLHAGGGLRWLA
                     RQFRPDTSGGVELFLVSRLGYERFYLDDGTRLGRPELGFGFGLQGRLYKKPRLAFRLD
                     FRALFTPTDTDSTLAVCRGRCMTESANTGFSTGIAIVW"
     gene            complement(20428..21228)
                     /locus_tag="VFV99_29005"
     CDS             complement(20428..21228)
                     /locus_tag="VFV99_29005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5063464.1"
                     /translation="MALSFLYGTAWKEDDTERCVRDALAAGFRGIDTANQRKHYFEAG
                     VGRAIAASGIPRQDLFIQTKFTFIDGQDSRLPYDPDAPIATQVAQSFAKSLEHLGVTY
                     LDSYVLHGPSQTHGLGDDDHEAWRAMEKLVGERGVRVLGASNMTAQQIGELYNFARIK
                     PAFIQNRCYAKTGWDVHSRNLCKQHNITYQGFSLLTANRQVLASPKVVAIAQRHGKTV
                     AQIVFRFAQQIGMLPLTGTTDPAHMREDLACSTITLTTDELAVIESAG"
     gene            complement(21238..21813)
                     /locus_tag="VFV99_29010"
     CDS             complement(21238..21813)
                     /locus_tag="VFV99_29010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063465.1"
                     /translation="MIGIPLALAVFGYGEWATHKYLLHGAGRDRRSPFAFHYHDHHQA
                     VRRNGGYDPAYEGPVWSTPTQAREAVGLAIVALAHAPLFPIAPFYTSTIWYCLHRYRR
                     VHRRAHLDPAWGRDHLQWHYEHHMGDQDKNFGVVWSWFDILAKTREIYVGTDKERAAH
                     TKHAVRAETAKSGAALRAQRRNPLRRLLRSL"
     gene            complement(21934..23730)
                     /gene="ltrA"
                     /locus_tag="VFV99_29015"
     CDS             complement(21934..23730)
                     /gene="ltrA"
                     /locus_tag="VFV99_29015"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX5063466.1"
                     /translation="MSEAERREPDPRREPRGRRQARGPQQQVKAAASTHLQSERAGCE
                     PAGRAAHVTAKATPTERDHNRADGLGGVWVAARVQGSSRNAREPSAQPPSRRGASYKP
                     KAKSSTAQRKSEGIIVPPAPATKAVQHNAAGGKGPWGDGRVDRRGTRKGMDAVRPNSP
                     GGRRPDDDVRRLQGRLRDVAKQQPGRKFHALYDRIYRSDVLYEAWNQVRRNQGAAGVD
                     AQTIAAIEQSGVERFLEAIQAELREHEYQPQAVLRRYIPKADGKQRPLGIPTVRDRVV
                     QAATKLILEPIFEVGFKDCSYGFRPGRSTTQALEKIRVTVNHGFNHVLDADIRDFFGN
                     ISHETLLSLVGRKISDRRVLKLVRQWLEARVMEDGLEKTMLSGTPQGGVISPLLSNIY
                     LDYLDGVWSKRCADLGVLVRYCDDFVVLCKTEKDCREAERRVRKLLRELKLELHPDKT
                     RRVDLGWGKQGIDFLGCHLRKRFSGPQWEKRRKRVYFLQRWPSTRSMKRIRRRVKGMT
                     SKSRCHVDPRDVIGELNPVLRGWGQYFRTGNAADKFSEIDKYVAWRLKRMRIRRKGRH
                     LKPGESHRWNASYFYTLGLHKLSGTVAYPEAA"
BASE COUNT         3992 a         7927 c         7888 g         4315 t
ORIGIN      
        1 gcaggcgact ttgatgaagc cgccggtcag ctcgaacttc tcggcgagcg cgtcgaagtc
       61 aatatccgct gaccgtgcgc gttccggcaa cgtgcgccgc cacagctctg cgcgcatgtc
      121 gggactcggg aacgtgaagt agacgtcgta cgtgaagcgg cgcttgaacg cctgatcgat
      181 cgcgccggtc aggttcgtcg tcaggatcgt gagcccgttg aatcgctcga gccgctgcag
      241 caggtagttc gtctcgagat tcgcgtagcg gtcgttgctg ctcttcacgt cggaggtgcg
      301 cttgccgaac aaggagtccg cctcgttgaa cagcagcacg acgtgcccgg gctcggcggc
      361 gtcgaacacg tcggataggt tcttctcggt ctcgccgagc cacttcgaca cgacctgcga
      421 cagatcgatc tcgtagatat cgaggccgag ccgcttcgcg agcacggtgc ccgacatcgt
      481 cttgccgacg ccgggatgac cggagaacag caccgagatg ccctgcgcac cgcgcagctt
      541 gaactgatcg cggatcttgc ggcgctcgcg tgcggctgcc gcgatctcga cgagtgcggc
      601 acgagtgtcg tcgtcgacaa cgagatcatc gagatcgaac ggcgtcggca gcttcttacc
      661 gaggcgcgag atccgctggt gcagctgcgc cgcgaccgcc tgatcgagat ccaccgcgga
      721 cggcggaccg gactcgggca tgcgtcccgc ctcggccgcc tgtgcggcag cgacaacgat
      781 accgcctgga atcgcgaacc gaccggcgag cccctcggca tcctgcgccg acagcgacgg
      841 cacggccgtc tgccacatcg ccgcgcgtac ctgcagcggt ggcacctcga tcgtgaggtg
      901 cacgagcggt cgctgatgaa tgcgcggcat gcgctcgcga ttgatcgtca tcgcgaccgg
      961 accgctccac tcgtcgagga acatgtcgaa gaacgccggc atcgcatcgc gatcggcgtc
     1021 gacgacggcc gcatcgtcga catcgatgaa cgccggcaac gcgtcgagca gcatcagctc
     1081 gcgtacgagc gagcgcacca tcgtctgctg cgccgcgagc tgcagctgcg agagccgctt
     1141 tgcatcgatc agcagcaagc gctgattcca gtgcgccgct gcgagctgga gcagcagctt
     1201 cttgccgacg ccgcgttgcc cctgcactgc gagcacgacc tcgttcgatc gcatcgcgct
     1261 cgtcgcgcgg tcgaccgtca cggcaggaaa cgcgccgctc accggaccaa agcggagctc
     1321 ggcgacttcc gcgagctcgg gatcgagctc gagcgcacca ccgtcgagga gatagacgag
     1381 gcgcgtcgca gcgcgaatgc gccggaacat cagcgaatcg ctctggccgg tgccgcccga
     1441 aagatcgagt agccggtagc gcaacagcgg cgagctcgcc gatagatcgc gcgccatcat
     1501 cgaccgcgtc tgcggcgtgt cgtagacgaa ctgcgcgagc aagcggccat cgagcccccg
     1561 cacgttcgga tcacgcgcga ggtagcggta tccctgcacg agattcggat cgacctcggg
     1621 catcagcgca aacacgagcg acgaccactg ccgcgccgtc aggttgaacg tgcggatcaa
     1681 atcgacgagc ggcgagcgcg tgcgcgcttc gatcgtcgcg cggatccgcg cctcggtcgt
     1741 ctcgtgcgcg gcgagccact gctcgagaac ttggtcggcg ccgctgtcac ctgtcacctg
     1801 cggtgcaccg cgcgcgatcg agaacagccg gcccatgtct tcgggtccga tcacatcttc
     1861 gccggcgagc ccgctctctt tgcgccagcc gagccggatc tggtactcga gccgcagcca
     1921 tacgcgacgc agctcgtcgc gtacgtgtgt cgcgacgtcg gtatacggct cggcccccat
     1981 tacccgacta tctcacggcg cggcgagctt caccggcacc gccatcgcct ggaccgtcag
     2041 aacttctacc tcgtccccgg tcggcgactt gaagctgcgg gtgtgcttcg ccgcttccat
     2101 atcgacgtcc gcggcgccgt tgatcgccgg atccttgtcg aaccacgtcg ccatcgtctg
     2161 gtcgccgagc ttgacgtcgt tcgcgagccg gatgtcgaac gcggcttcct tctgcttcgt
     2221 cggatcttcg cgccaccacg acacgtcgag cttgcgcagc gtcacgcgtg cacccgggcc
     2281 gagcttgagc gcgtacgtcc cggcgcggcc gaggaagcga ccgaccgcaa gcgtcgtcgc
     2341 gccggcattc acggttagct gctcgagctt gtcgacgcca cctccgctat cgaccggaag
     2401 caccgatgga atcagcaccc accgcggcgt ctggtcgtcg ttcttgagct gaacatcgac
     2461 gagcaggtgc tcggtcggcg gacggcctgc cttttgacca acgagcgtga cagtcaccgc
     2521 gccgttcatg ttcgttcctc cgttcgaccc attcgactcg cttgccgagc ccgagcccgt
     2581 gctcacgcgc ggcggtggtt ggcgcccaca actcgctgcg atcagcagtg ccaggagcgc
     2641 gactctcacg cgatgaagat agcgcgatta ctcttgatcg cgcgttttct ggacccgctt
     2701 ctcgtcgagc tccataccgg cggtggccat gaagccacct ttgcgctcgg taccaccgag
     2761 gctgtacggc tgtgactcga ggatttcctt cttcgtgacc ttgtcgggga acttgcccga
     2821 cttcggatcg tagagcgggt cggtgaactt gccggacgcg tccatcgagc cctgttcctt
     2881 catgatgcgc tcgtcgtagt cgttgcgacc tgcgttgtac gcacgcacca tctcgtcgac
     2941 catcgcgtct tccgtcgcgg tcgcgccgag accggaccac agccgctcgc ccgcgtcgtt
     3001 cttgcccatg aagagcgcgt tctcgccttg ccacgcgcca cccggatgct tcagcagata
     3061 cttcgggtga ttgtagaagt agtaccactg gccagggacg agctctttat cgagcttcgc
     3121 gggctcgacg ctgccgaact ggccgccgtt cttgtacgcc tcgtcggcga cctcggtcga
     3181 cttcatgaag ttcgcgaccg gcgtgccaga aatgccagcc ttgacgtgca tgcgctgctg
     3241 cgcgggaatc gccttgtcct ctttcggatc ggcggagccg aacatcttgt cgaacttctt
     3301 gtcgccgatc gcggcgcgca gcgcatcgat ctccatcgcg atcaccgacg acaaacactc
     3361 ggcgactgtc gcgcccgcga gccactcctt gatcgccttc gacgcgctgt ggcctttctt
     3421 cagctcccag tccatccagc gacccgattc gtgcgggttg acgaacgcct gcgcttgcgt
     3481 gtggccggag cgatacttgt cttgccagag ctcgggctgg cccgcgttct cgcactgccg
     3541 ctggtagttg taccagtagt gaatgccgtg attgacgtcg ttctggccgc cggagccggg
     3601 ctcgacgttg gccttctgca tgtccttgac gacgacgcca cccatcgctt ctttcgtcgc
     3661 cggaatctcg ggaccgcctg cctgcggctg cgcagcgaac gcgcgatccg atgccgacgc
     3721 gctcgcggtc gatgccgtcg tgtcgaccgc aggagcatga ccaccggtga acgtcgtgcc
     3781 ggacgtcgtt tcgctgtgtg ccgcgtcggt cgtgccggcg tcagcaggtt gcgcgcggcg
     3841 ggtatcgtgt tcgcgggcca tcggaatctc cggcggttag gtcatgcctt ttacgaggta
     3901 ggacacggga tccttccgtg gcaaatcgcc tgtgatttca accaagtcga atctgcaggc
     3961 tcagacaatc gggctcgctg acgatgacga cgtcgccatc gcggagctcg gcgcgtgtga
     4021 tccgcccgcg catgacgtcg ttgatatagg tgccgttcgt cgtgccctga tcgatcgcga
     4081 cgagcttgcc ttgatcgatc ccgatccaca cgtgcttgcc cgacgcgcgg tgatcattga
     4141 cgagcacgtg cgcgacaccc ggctggcgac cgatgatgat gccggtcgcc gtgagcgcga
     4201 agcgctgacc ttgcagcgag ccgcgcgtgc accacaggct gccgatgctg agcgaaccga
     4261 acgcagtcgg tgcgatgccg cccgattgat tcggcccact gattgcgacg gtctttgcga
     4321 ttggttgctg cggcaccggc atcatcggcg gcgcgttctg cggctgcacg cgtccgtaca
     4381 tctgctgttg cggcggcgcg aactgcggcg gcggcggata gccttgctgc acgtgaggcg
     4441 gctgcatatg tggcgcgccg ggtggcggca tgtgcggcgc cccgggcggc atatgcggtg
     4501 cgccaggcat cgcttgctgc ggtgacggtg ctgctttctt gccgcgcttc gcgagcatga
     4561 ggccgccgcc accgacgaca acgaggccgc cgatcacgag accggcgatc agaccgccgt
     4621 tgcctcccga tggcttcttc tccttgcctt ccttcttcgc ctcattcgat tgccggatca
     4681 gcgtgccggc ttcgctgtgc atcgggaaca gcgtctcgac gtcgcggaag ttctcgatcg
     4741 cggcggtgta ctcgtcggcc cagtagtgct ccagaccctt ctgccactcg gcgagcgtct
     4801 tgctcggctt cggatcgagc ttctctttct tgacgaattc ctgcagcgtc gcgctcgcga
     4861 cgaggaagtt gaaaccttgc acgtcggtgc cgaacgtcgc gagcccgatg acgttgccct
     4921 tcatgtcgag tgcggggccg cccgaattgc cgtgcgtgat cggcgcgctg atctggagga
     4981 tctgctcgcc actcgtcgcg tgcttcagcg acgacaccgt gccttcggtg acggtcgcct
     5041 gcagctgcga tttctcgtcg agtagctcgc gcaggtcggc gacgccggga tagccgatca
     5101 ccacgatctg atcggcgacc tgcgagcgcg tcgagtcgcc gatcggcagc gcaggcgcgt
     5161 tgtcgatctt caccttgatg atcgcggcat cgcgcccggt gccgggtttg ccgtactgct
     5221 tgacctcgta gtcgagcttc tcgccgctcg gcagcacgac gtacgcgcgc gtctcgaggt
     5281 tgacgagctt gatctcgctg agcagatcgc gaatctggcg gtcgctcatg cgcgccatct
     5341 cgggcccgag cgcgcgtcgc acctcgctca acatctgttg atagagcttg cccttcgcga
     5401 cgacctcgcc gtctgcgacg tcggaaacga cgtgcgcgtt ggtcgcgatg taaccgtccg
     5461 cggtgatgaa gaagcccgtg ccgctaccac cgaccgcaca gcagttcggg atcttctggc
     5521 cagcgatctc gtagtcggca acgtacgcgc cccacacgcg aacgaccgat ggcttggcgg
     5581 tcagcgcgat cttctgttgc tgaggcggtt ccgcgtgcgc ggtgccaagc agagcgatca
     5641 gcaatgcgca cacgaggcgc acgacctgtc ccatcgagcc gccatcgtac cgtatcctcg
     5701 gcagcgtggg cttcggcatc agccgtcacg cacgtagtcg taagggtcgc ggactgtttc
     5761 ggaaggcgcc cgaccccaag gaggtcggcg accggctcgg caggcttgca cgccggatgc
     5821 tgcgcgacgc cgtcgtgcgt tcgggagcga agggcgaccg cggcgagtac cgttacattg
     5881 tcgagctgaa gatgcatccg gtggcgccgg ccgggacgct gagtgtgcag cccgacggtg
     5941 aaatcgtgct gaaggtggaa acgtcgacgc tcggccccgg ctaccacgcg cacgtgatcg
     6001 acaaggtcac gccgatcctc gaggagctcg actacgtgtg gacggacacg ttcgacccgg
     6061 cggcgacgca ggccgcgatg tgcgcatggc ttgcagacga gctgcgcggc aacgacaagg
     6121 tcacgatcgg cgtacccgac acgcgacgat ttcgcgtcga cgcaccggtg ctgaccgcgc
     6181 tcggcgcgcg cgatgcggcg tggcgcgatg cggtgctggc cgatcccgcg aaggcggccg
     6241 atgcgttcgc gtggtggcaa accgggccag gacgcgacgt gctcgcgcgt gcattgatca
     6301 cgatgtcgct cgaggtgccg tggcgcgagc cgctcgacaa gggcgagcgt gagctgatga
     6361 cggaggtcga cgaggatctg cgcgcagcgc gcaaggcgga tccggcgctg ccactgccgt
     6421 acccggagtg gaaagagctg ctgatgcacc tcggtatcga ggacgaggac gttaacgaga
     6481 aggtcggcga tcgcacgtcg acgctcggct accgccgtca cgacatcgag atcgagctgt
     6541 cgggtggctg gctcgtcgtg attcccggcg cgttcgccgg ccactgggaa gacgacggcg
     6601 cgaagtactg ggcgaccgat ggcgatcgga tgatcgagtt ctcgtcgcta accgcgaacg
     6661 acgaaactga ctcggccaag ctgctcgcgg tggcacctga aaagtacgaa gtcgtcgagc
     6721 gactcgtcga cggaaaccac cacggtcgcg cagagacctt cgaggaaaac gatgtaccga
     6781 ttctcgtcgg cctcatgtgc aatgccccgc acgtgggaat cctcacgtgc aagggcggca
     6841 ctcgagaatg ggcgctcgca acctggcgca gccttcagca gaagaattaa ccgccacgac
     6901 gccgtcagac gccagccccg aaaaaatctc tcggatcttg gcgtgcgccg gcgtcgtggc
     6961 ggtcgatctc ttagtgcgtt agcaccgaca cgctgcgcgg cggcatcgtg atcgtgaacg
     7021 agccgtttga tacatcgacg gtccggctac ctgggtcgag gcgatccgtc agcgtgccgt
     7081 tcccgatggg agcgctatgt acggtcaacg tgcgcgcggt ggcggcgtgg ttcgccacga
     7141 tgatggccgc ttcgctggcg gtgacccgcg cgaacgcgag cacgtcgtcc gtcgaattca
     7201 gctgcacgta ggcaccgcgg cgcagcggct cgagcgtctg gcgcagcttg ccggcgcgcg
     7261 cgaccttgtc gaacatcgcg cgttgtcgcg gcgtgcgcgc cgatggagcg gcccacatgt
     7321 ggcggttgtc cggatcggcg ccgccgtgct cgccgtattc atcgccgtag tagaggagtg
     7381 gcgcgccagg cgtcgtcagc agccactgca acgcgaacgc agcgcggtcg tacgggagat
     7441 ctgtcgtcgg cattgcgggc aggccgtcgc tcgcccactt gtgatggacg atcgtgctgc
     7501 ccgagccgag atccgcgagc gagacgagcc gctcggtgtc gtgcgagccg acgtacgtcg
     7561 acatcacggc gtcggcggga tagtgatcga ggctcgcctt cgtccagtaa tcgaggtgca
     7621 gcatgccgcg cgcctcgtcg gcgaacacgt tggtcgtggt cgcgtgatag agcacgaagt
     7681 cgaactggcc cgagagcgcg aacggcccga tgtagcgcgc gatcgtcgcg tagtcgttga
     7741 gcgagttgtt gatgtcgtcg ccgccccagc ccatcgcggt ctcgccgagc aggtagtact
     7801 ccgtgccgcc ggtctcgaag cgctcgtgga tgcgcgtcga gagattgctg atggcgagat
     7861 cctcgacgtg cttgaccgcg tcgacacgca gcccgtcgag atcgaagcgc tcgagccacc
     7921 actcggcatc ggcgatcatc tgctcgccag catccttgtt ttgccagttg acgtcgggca
     7981 tgtagggatg gaacgagcag tcgaggcggt gctcggtcca gtcgcagccg ggcgtgccgc
     8041 attcgcagcc ggtgcggaac cagtcgggat gcgcagcgta atactcgtgc tgctcgtgga
     8101 cgtgattgat cacgaagtcc atcagcacgc gaatgccgtg tttgtgtgcg gcggcgacga
     8161 gcgcatcgag atccgcttcg ctgccgaggc gcggatcgat cgcgcgcgct ttgaccggcc
     8221 agtagccgtg gaacgcggtg acaccgtggc cgttctcgta ctcgacgcgc gtcgtgtttg
     8281 cgacgaacgg cgacagccat agcgcgcgca cgccgagcgc atcgaacgtg ccgtcttcga
     8341 tcgccgacgc gacgccgcgc agatcgccgc ccttgtattc ggcgcccgga tcggcgcccg
     8401 ccgagggcgc gggattgttc gtcggatcgc cgtcgcgaaa ccgatcggtc atcaccatgt
     8461 agatcagcgc gtcgcgccaa tcgaacggat cggcctcgat ccagaacggc acgagcacgg
     8521 gctcggcgct cgcaccggcg gcatccttcg cggtgatacg cagcgtgtac ttgccggcgg
     8581 cgagacccgg tgtcgcgatc gtcagctcgc caccgctgcg ggtgaagtcg atcggcgcgc
     8641 cttgcagcgt caccgcgatg tcggtgatcg cggctgcgct cttgccgcgc gacacgcgca
     8701 cgcgcgtcgt gttggtgtcg tgcgagatca cttcgagtag cggcggcgcg caatcggcga
     8761 cgcgcagcgc ggagttctcg acgccaccgt catacgcgcg atacgggttc tgcggatcga
     8821 gcacccagtc gaccccgccg ccgtcatagc cgacaacgag cttgtacggc cagatgccgg
     8881 gagcgagctc gagctcgccg gtgaacgtgc cgtcgtggtc gggatctgcg agcgccgtcc
     8941 gcgccgacca gtcccaggcg ccgacgatgt ccacggccgt gacgccggca tcgccgtggt
     9001 aggtgatcga gctcgtgcac gagcgcagcg gggtgctgtc gtcggccggc gcatccggac
     9061 cggtgccgtc gtcaccaccg ccgtgcaggt agggcggttt gcacgcgacc agccccagca
     9121 ccacgagcag acgcagatga cccatacacg cgcgcgaagc aaggcgaacg ccacgctaag
     9181 ccgctggaag atcgtgggcg cggactagcg cttgttgccg aacgcaacgt gggttcgcgc
     9241 gctctgcggg gcgttgccga tgccgagcgt cgtgttccag accgcttcga ccaggcagtc
     9301 gtgcagtgtc gggtttgccg gtgtcaccgt gacctcgacg gcgacgatct cttcgagcgt
     9361 ggtctcgacg tcgatgccgc cgcgcgccgt gcccggattg cagctggtga tggcctgctt
     9421 cagttgcgcg ctcaggtcga ggcgctgaag tagatggccg atgctggttc cgatgccggt
     9481 gtcgtgactc gtcgtgccga tgcggccgaa gccgaacgtg ccaagcccac cgatgtcctc
     9541 gtagccaccg tcgccgcccc actctccgaa cagcgaccag accgagttga tcgccagcgc
     9601 gacctgatcg acctgctcct gtaggtctac atcgaggacg tgcatcaccg agagagcgcg
     9661 cgcgagcgta cgtgcttgcg tcgggtcggc acgcaccacg cgcttgatcg gcttgttcca
     9721 gagcaggccg gcgatcgtga tcggtccggc ggcgccgtcg ccttcccccc accacgtgca
     9781 cgtcatgccc tcggcaagcg aatcgccgca ctcgtgcatg tcggcgaagt ggtcgacggt
     9841 cttccagccg gtcgcggaca cagcaagatg atcgatcgag atcgggcgca gcagcatcgt
     9901 cgcgtcaggc gtggcgccgt cgtcgacgcc cgcgaatacg gcgatgccct cggtcgcttt
     9961 cgcgagcgtg gcgagcaccg actcctcgtc gtcgaagcgc gtgatgcttc cgcccgtcgt
    10021 cggatgcacg acatgcacga ggacgccgtc cgggacgagc gcgcgcaacg cggcggactc
    10081 gccgaaccgc tcggcgagcc gttcgtcgct gaacagcacg atccgtttgg taccctgcac
    10141 gcgcgatagc cacgcgaccg cctcgccgag agcggcgtcg acgttcgagc cattacgcgg
    10201 tggcagcgca cgaatcgcgc gatcgatctg cggcgccgcg tgcgacgcga ccatccagct
    10261 cggcaacagc ggctcggcgg tgcgcgcgta gccgatcacc tgcacgtggc tcatcggtgc
    10321 accgcgcacg tacgcgagtg ctgtcgcgcg ttgtgcctcg agctcgtcgg tgctgagcga
    10381 gcgcgagtga tcgatcacga tcgcggtgtg cagatcggca ggcacgtcgg tgagcacgct
    10441 cgccacgtcg atctcgacgc gcgcgaagtg cgtgctgtcg agcggaagcc ggcccgcgat
    10501 ggtgccgatc cggtgttcgc ctggggactg cttcatgagg tcgcgactgg caacgccgat
    10561 ccagtgcgat gtctcgtcgc cgctgtcatc acagatctcg ccgacgccat ccgtcggaat
    10621 cgccgttcgt tgcttcgccg gcacgcgttg ccaccagctc tccggcagct cgacgaagcg
    10681 cgcatccttg acgaagcagg tgccgacctc gagcgccaga tcgagcacga cgtgcgcgtt
    10741 gtgtggcgcc atcagatcga gcgtcacctg gtcgttgccg ccgtcgatca cgaatgccca
    10801 cgagcggtcg ctgccggtgc ccggtttgag cgtcagcgct tcgaatgcct tgtcggcggc
    10861 ctcggcattt tcgagacgca gcgcgtgacg cgtaccatcg accgtgacgg ttgccgccgt
    10921 caccacaccg ttgcgtggaa tcgcaaacgt atgcgcgttg agctcgacgc cgggcccgtc
    10981 gacgtcgaac gcgaagcggg cctggatgcg cgcggtcgcg ccgccgtggg tgatgcggat
    11041 cgcggtcggt ttgtcgaagg cgtcgtcgtc accgtcgtca gcaatggcct gccccgccag
    11101 gaaggcgagc acggcgagag ccgcgagacg gcgcatgtcc ctgtgacacg ggcagggcgc
    11161 tgccagttcc atcaatgtta gggttcgccc aatgggcaac aaattcggga tgtcgccgtt
    11221 cgacatactt gcggccttcg gtcccgtgct ggtcctcggg ccggtcgtgc tctggatgtt
    11281 gttgttcggg cagctgctca tctacccgat cgcgcgctgg aaggcgcagc gtgaagcggt
    11341 catggatccg cagctcggca tcaagttcgc gctgcatttt ttcgcgctac tcgcatttca
    11401 gatcgtgctg ttcgcgctgg cgaccgcgct ctacacgctg ttcagcaagg ccgaggacaa
    11461 gggcgagctg tatcgcatgg cgttcggctt cttgatccca ggcggcggcg tgctcgccgc
    11521 gcactacgtt ctgctcaagc gcacgaacca agatcaattc cccggcgtgc gccggttgtt
    11581 ccttggctac aacctgctcg tcactgggtt gatcggcttc gtgatgctgc tcgtcgcgtt
    11641 ccaggtgttc ttcaggagag gaccggccgg caacgacggg cggttcgcga tcgcgagcgt
    11701 gctcgtctat gtcggcgcgt gggtcgcgtg cggcctccat ttcggcaagc tcgtgctcgg
    11761 cgattcggct gcaccgccgt cgaacgtgat cccgccgtcg gtgccgacgc atgcggccgc
    11821 gcaacagagc ggaccggtgc tgccgtcgct cagtgcgggg acgtttccgc cgatcgatcc
    11881 gaagaagtga tcgcggccgg gtggatctct ttggggatga agcggcggtc gacgatgtcg
    11941 ctgacgtcga gcggcttctt cagaatgccg gccttcaccg cgtacttcat gatctgcgtc
    12001 atgtcctcgt cgctcggcgt cagctggcga tagctcacgc gatcgggcgg ttgcgtcagc
    12061 acgaactcga gtagcttctg atcctggcgg aagtacggcg ccgcgacctt cgcggcctcg
    12121 agccgatgtt gctccgccca ctcgccgctc tccgcgatgc cgcgcacgag gtcgcgcacg
    12181 atctcgggat gttcgttgat cagctcgtcg cgcacgacga ggacgcacga tacgaagtgc
    12241 ggccagatgt ccttcgcgta gtagaggacg cggccggtgc cgtcgagctc ggccttggcg
    12301 gcgtgcggct cgccgatgaa gtaggcgtcg atcgcgccag cggcgagcgc cgacggcatg
    12361 tcgggtggcg gcaaatcgac gaagttgatg tcgccgggct gcatgccctc gtcttccatc
    12421 agcttcgtga tcaccaggta ctggttcgag aaccggctcg gccgcgcgaa cgtcttgcct
    12481 ttgagatcgc ggatcgattt cgcggtcgag ttcttcggaa cgatcacggt cgagccatcg
    12541 cgatggccga ggtacacgat atggatcggc acgccttgct cgcgcagcac cattgcgagc
    12601 ggtgcgagca ggaacgtcgc ctggatcttc ttcgccttga gcgcctcgac caccgtcgga
    12661 aactcggtga accgctgcga caagaatcgc gtgccggtct tcgtcgtctt cgtcgcgaag
    12721 tcggtgacgg ggcaggtgag gtgacacgtc accggcaaga agccgacttg caattcggca
    12781 gcgctcgcgg tcgccgagcc gcgccacggt ttcttgtgcg cgagcgtcag tgtcgcgagc
    12841 actccgatgg tgatcaacgt gaggcgcagc ttgtaactca tgacagtccg agctcctcca
    12901 tgatcgcctg ttctgcctcg gcaacgccag gcgcagcagg cagtcgcgag cggccgggcg
    12961 gtagctcgag cgtcttgcgc acgtttgccg ggcgcgtccc gagcacgatg acacggtcgc
    13021 cgatctgcac ggcctcttcg atatcgtgcg tcacgaacac gaccgtgcgc ggtcgttcgg
    13081 cgagcgtcgt gaccagctcc atgcgcatcc gccgccgcgt ctggtagtcg agcgcggaga
    13141 acggctcgtc catcaataga atgtcggtct cggcagcgag tgcgcgcgaa atctcgacgc
    13201 gctgcctcat gccgcctgac agctggtgtg catacgcgtc agcgaagtcg gcaagccgcg
    13261 tgatcctcag ccactcgtcg accttcgctg gattgccgag cgcgatgttc tggcgcacgg
    13321 tcagccacgg caagaggccg ccgtcttgat agatcgtgcg gctcgcgcct ttgcgcacga
    13381 tcgtgccgcg cgtcggctgc aggtgaccgc cgagcagcga cagcagcgtc gacttgccgc
    13441 agcccgaggg gccgacgcac acgacgagct cgcccttgct caccgtgaga tccaggttgt
    13501 cgatcaccga cagggcgccg tagtcgaagc cgacgccgtt caattcgagg acgacactca
    13561 tcgctcgaac ccccagcgca agctcggaat cttcgcgagt cgcgagaacg cgaggtcgag
    13621 gccaattccg atcgcgccga tcgtgatcat gccgcacacg acgagatcca ttcgcagacc
    13681 gttgcgtgcg tcgacgatca ggaagccaag accgctgcgc accgcgatca tctcggcagc
    13741 gaccaccacc atccacgcga cgccgatcgc gacgcgcagc gcgatcacga gctgcggcat
    13801 gatcgcgggc agcgtgatgt gaaacagcac ctgcatgccg cgcaggccga tgtcgtcggc
    13861 aacgcggtag tagaccttcg gcaccgatgc cgatgcagct gcggtcgcga gcacgacctg
    13921 gaagaacgtc gcgaggaaga tgatgaagat cgccggtgga tcgccgacac cgaaccagat
    13981 gatcgcgaac gggatccacg cgatcggcga cagcgaacgg aagaagttta cccacggcat
    14041 cagcgcagca cgcggcgccg catgtctgcc gatcacgagg ccgagcggga cagctgccac
    14101 gacagcggtc aagaaacccc acgcgacgcg atacagcgaa gcgatcacgt cgtcgagcaa
    14161 ccggccggac gcgagctcgg cgcgaaacgc gtgaccgacc gcttccggcg acggcacggt
    14221 tcccgtcgga aacatgtccg ctgccgacgc agcgccccac gccgccagga tcaccaaccc
    14281 cgtgatgatc ggaaatccga tattcgaaat gtaactacga cccttagcag ggactctcac
    14341 cgcgcacgca tcgtgacaca tccggaggac gcgaggttgc gttgtcgcga aggacgcggc
    14401 taccgtcgcc ggcatgaaga aagtcgccgt cctcggatcg ggtcaagtgg gtgatgcgtt
    14461 gtcgaacggc ttcctcgcgc acggctacgc ggtcatgcgc gcgacacgcg agcccaagaa
    14521 gctggaaagc tggaagacca gcgcaaaggg cgaagcggcg atcggcagct acgacgaggc
    14581 tgcgaaatgg gccgacacga tcgtgctcgc ggtcaaaggg ctcatcgccg accaagtgct
    14641 cgaccagtgc ggcctcgcga acctcgccgg caagacgatc atcgacacga cgaacccgat
    14701 cggcagcgaa ccgcccgaca acggcgtgat ccgctacttc acgaacgcga acgaatcact
    14761 gatggagcgc ctgcagaaga aggtgccgca ggcgaacttc gtcaaagcgt tcaactcggt
    14821 cggcaactcg ctcatggtga acccgaagct cgacacgcag ccgacgatgt tcatctgcgg
    14881 caacaacgcc ggcgcgaagc aggaggtcac cgagctcctg acgaagttcg gctgggagac
    14941 ggccgacatg ggcggcgtcg aaggcgcgcg accgatcgag tcgctgtgca tcctgtggtg
    15001 cattcccggc ttcatccgca aagagtggat gcacgcgttc aagctcgtca aagcgcgcgg
    15061 gtagttgttc gatgcggctt agtgtgtgtc gaccgttcct ggcgcttcga aggtcggtga
    15121 cgcagctgct ggtgtcagcg tcggcgaaac gcccgccggc gtgaatgctc ggaagtagcc
    15181 aacactaccg ccgccaccac caccggaggg tgcagagggt ggtgctggag taaggcgaaa
    15241 cttgattcca ttcgtcgggc ttgtctgagc gcagccgcca tcgccaccgg gagaggcgtc
    15301 ggtcgctgtt ccacctaacg cgcacgtcgt cgtcgccatg ccgtcggtgc cagctacgcc
    15361 gacagagttc actgtggtga atggttttcc gctgccgccg gcaccgccgt ttgcaaacac
    15421 ttgaccggtc acggtcacca tgcggccttg caacacgacg ttgccgccag cgccgccgcc
    15481 ggcaccgccg taggggctgc tgccagaatt gccaccggcg cctccaccac caccggcgtc
    15541 aatgattccc gtcacgctga cagttccgcg gcacgaaacg agtgtcacgc cgccgccgcc
    15601 gtaaccgcct cgactcgaag tgctgccgcc accgcttgcg cccggaagaa gtcccatcgc
    15661 ggcggccgca tcgacgattg cgccaccgtt gttagcaccg ccattggtgt tcgaatcgcc
    15721 gccagcgccg ccgttggtct tgaagcctgc gccgcctcca ccggctccgt tcgtgttcga
    15781 cggagcacct cctgatgtca tgtaatcacc accgggtccc gagtcggtgc tgtcatctgc
    15841 gctgacgtcg agaacgccag cgaccgtcaa ggatgtgtcc gtaacgatcg cgagtgcgaa
    15901 tccgccagtg actcgaaacg tcacgctcgg ttcgagctcg aacgagctgt agcgaagcag
    15961 gcagatcggt ggacccgctg actgtgtaac catcgctgca cacgagttgg tcgtctccac
    16021 tgtgctcgtg ctgatggtgc gtgcgcccgt cgcggctccc tcacacgcgt ccggtacgta
    16081 gcgtggcgtg atcgttcctg gcttgcacgt ttgggtgacg gggctgcaaa agccagtggc
    16141 gcactcgtcg tccgacatgc aaggacgaca tgcgtatgac tgtgggtcgc aaacgcgatc
    16201 accacattgc gacgagtcga gacattcggt gcagacaccc gtgggcgcgc agtgcggcgt
    16261 tggatcgctg cagtcgcttt ctgcttggca cgacgtcggt gcgacgcatt cgttgttcac
    16321 gcacgtttcc ggtgcgtcgc acgtcgtacc ggctggcaag cctttcgagt cgcaatccgc
    16381 ggccgtcgta cagcacacgg caggatttcg cttcgtgcac gccgtcgaca acaaaaacaa
    16441 aaggagcagc aatctcatgc tgctcccgat gatacggcaa cgcgactgct aggcgctgca
    16501 actatttgtt gctactcgag ccagatcgcc gcgacgtact ggctatgagc cgtcgagtac
    16561 gtgtcgatga agtcgggcga ccactggatg tgcacccaga tcgtcgtcgc cgcgccgacg
    16621 gttgacgtcg tcagcacgtc ctcgtgatag cccgagtcgg tgcccacgcc gagcttattt
    16681 gcagtgaccg tggggtcgcc cttccagatc tcgacgaagg tgtcggtcat cggatcgcca
    16741 gctgtcacga cgtgaacctt cttggtcgtg ctgttcgccg gcaccgtgaa cttgatccaa
    16801 tcctcgtcga ggtcgtccga gatgctcgcg ttggtgagca ggatcgtcga gttcgtcgcc
    16861 aggttctgcg ccgccgcaaa cgtgtcgttc gcaccggcag tatcggcttc cgccgccgcc
    16921 accagcgtct gcgggttgac gcggatcgcg tagctgtagc ccgacgagcc gctcgcgtcg
    16981 gtgtagaccg tgaagtactt gcccgcggtc tccacgatga cgttcggctt cgacgaggcc
    17041 gcgacggcgt ctgcgaaatg gccgctcgcc ggcaggatgt aaacaccagg cgtcgcgttc
    17101 gggtcgctcg ggctcaccga gtagcgcgac accgacgtcg cggccgcatc gaacgagtag
    17161 aggtgcgaat cgaacgctgc catcaccgtc gtggtggtcg ccgtgcccgc cgcgagcgca
    17221 accggcgcgc gagccgggaa gtcggtcggc gtgcccatcg ccgaggtcac ctgctggtcg
    17281 gcgacgtccg ccggaccgct gacgaagctg atgtcaccgg ccattgcatc gacatcgacg
    17341 aacagcgtgc cgctgacgcg gaacggctcg accgtatcga cgaccgcggt cacgccggcg
    17401 ggcgtgttga cctgcatgtt cgtatagagg aggcagatgc cgaagatcga tgcgccacac
    17461 gtcgcatcgt agaggttcgc gagatcgagg ttggtcgccg tgaacgtgac gaccgagccc
    17521 tgcgcgagcg tgcccttgaa gtcgaccttg accggcgact cgagcttgaa cgcttccttc
    17581 atcgtgagcg tgtcgttgcc gttgtgcacg acgacgtcat gggtaccgag cggtgcagtg
    17641 tcagcgatcg tgatgtcggc gaacagcgcg gtcgggctcg acaccgtgac ggccgagacg
    17701 gtcacgcccg agccgaagtc gagcgacacc ttgccgtctt cgaacgcgac gttatcggcg
    17761 ctcacttcga cccgcacctt acggccgatg aagcccgacg acggaaacac gccattgagc
    17821 gattgatccg gatcgtctcc gcagccaacg acagcgacca acccgagtac caacgcaaag
    17881 ctcttcaaac gcatatgcac cttctccgtt gaggcggcgc aaactagtcg agggctcgtc
    17941 ggtactcaat acaaattcgc agtcacagcc gtgacggcac gcacggcgca aaacgcgcca
    18001 aaacccgtca aaaaaaaaga gccgccggca cgacgccggc ggccgactca cgcccatgcg
    18061 gcaagctgcg tgagtccgaa gaatgcttgg tgagcagtca ttgcacgcag ggcgccagct
    18121 gcgcgcgcat gggtgccgag gtacaggcgc acgtcagcag cgcacgagac cgtgcgggcg
    18181 gatcacggga aactacggcg catccgcatc gcggaaacga atttgcggga tcggcgcgta
    18241 cttgaacgct ggtacatcgc ttgctgattc cctcggcaag cggcaagcgg caacgctcct
    18301 ccgccgcctg cgcaacaaca cccgcagatt tgcggagagg gagttcgttt atgcgtttta
    18361 tgaaaggtct actcagtgca attgtcatga tggttccggt cgtcgcgttt gccgacgagc
    18421 cggcgccgaa caccggtacg ccggaagcga ccgcggctcc gactccgtcg ggccagctgc
    18481 cgccggcaga atcggataag gacccggcac cgccggttcc gacgccgaac attccgggcc
    18541 tcaacggcga catcgtcgag caggcgggtg tcggtggcac ggtcggttat ggccgcgccg
    18601 gcgtgctcga gctcggcggc gctgccggtc tgacgctcgc gcaggacatc cgtgcggtga
    18661 acttctcgcc gtcgatcggt tggttcctcg tcgataacct cgagctgtcg gcgatcctcg
    18721 acgtgacgaa cctgaaggcc ggcggcgagt cggcgacgct gtggtcggcg ctcgcggagc
    18781 cgtcgttcca cttgccgatc aaccgttcgt tgttcggctt cgtcggtatg ggcgtcggtg
    18841 ctgcgtacgt gagccagctc ggtaccggtc tcgcggttgc gccgcgcatc ggtatggacg
    18901 tcatggtcgg gcgctcgggc gtgctgcgcc cgtcgctggc ctacgagtac accacgcacg
    18961 acgcgatggg ctcggtcgac gcgaacggca acaccgacgt gacgctggtc gcgatctcga
    19021 gcgcgctgcg cttcaacatc ggctactcga cgatgtggtg agcgctcgct agctagactg
    19081 ttggagcacc gccgggaaac cggcggtttt cttttgccct cagcttggct cagttgcccc
    19141 acgccatgcc gagacccatc atgccgccga cgccgccttc gatcgccacg gcgcgcgcga
    19201 gtagctcgat ctcgaacgtg cgcgacggcg aatcgtcgct gcggctgtag atgtcgtagc
    19261 cgaagcgcag gccaccgagc ccgccgggca cgaacgacac accacgcatg ttgtcgtcga
    19321 cgagcgcgaa cgtagcgccg agctcgccat cgatgaatac ccgcacgctc cggccgagat
    19381 tcttgccgag caattcacgg cgcagaccga gcgaagcacg gtgccccgtg ccgtgacgct
    19441 cgggcctcgg cgccatgctc gtgagtgcgc ggtcgtccat ccacgtcagc cacagccact
    19501 cgtattcgcc gaacacgcgc gtcttcttga acaccggatg ctcgacgccg agcccgatcg
    19561 atagcgccgt cgtgcggccg ccatcgagcg gcagcgcacc cgcgctcatg cgaaaaccga
    19621 acatgttgaa cgccggatcg gtgtcgacga ccggtgccgg ctcgctctcc acgtagtcat
    19681 cgggtggcgg tggatgcgcg gccgcgaccg gtgtcagcgc gacgaggccg agcgcgacga
    19741 gcgtacgagc ggcgagcttc accatacgat cgcgatcccc gtcgagaagc cagtattcgc
    19801 gctctccgtc atgcagcgac cgcgacacac cgcgagcgtg ctgtcggtgt cggtcggcgt
    19861 gaacagcgcg cggaagtcga ggcggaatgc gagccgcggc ttcttgtaca gccgaccttg
    19921 caggccgaag ccaaacccga gctcggggcg gccgagtcgc gtgccgtcgt cgagatagaa
    19981 gcgctcgtag ccgagccgtg acacgaggaa cagctcgaca ccgccactcg tgtcaggacg
    20041 aaactggcgc gcgagccagc gcaggccgcc gcccgcgtgc agcatgcggc cgctcacgct
    20101 ggtgccgggg ttcgcggtcg atgtcgaaac gttcgaggtc gcgaagccgc cttcggtgaa
    20161 gtactgccag cgcccgctgc cgagcgcgag ctcgagcgtg gggccgacgc ctccttcgga
    20221 gaggccgccg atgcgcgtgc cgtggccgcc aaacgccagc gcgatcgatc gcttcgacac
    20281 cggcatgaac gcgccgtcct catcggtgtc gtcatcgtcg gcacgcgcga cgccggccag
    20341 cgcgacgatc gtcaagacgc aagctaagac ccgcacggtg aggggtgcaa acgcacgaca
    20401 ccggcccgcg atctaatctt gggcgggtta gccggcggat tcgatcacgg ccagctcgtc
    20461 cgtggtcagc gtgatggtgc tgcaggcgag gtcctcgcgc atgtgcgcgg gatcagtggt
    20521 gccggtcagc ggcagcatcc cgatctgctg ggcgaaccgg aacacgatct gcgcgaccgt
    20581 cttgccgtgg cgctgggcga tcgcgacgac cttggggctc gcgagtacct ggcggttcgc
    20641 agtcagcagc gagaagccct ggtacgtgat gttgtgctgc ttgcacaggt tccgcgagtg
    20701 cacgtcccaa ccggtcttcg cgtagcagcg gttctgtatg aacgccggct tgatgcgcgc
    20761 gaagttgtac agctcgccga tctgctgcgc ggtcatgttg ctcgcaccga gcacacgcac
    20821 gccgcgctct ccgacgagct tctccatcgc gcgccacgct tcgtgatcat cgtcgccgag
    20881 accatgcgtc tgcgatgggc catggagcac gtagctgtcg aggtacgtga cgccgagatg
    20941 ctcgagcgac ttcgcgaacg actgcgcgac ctgggtcgcg attggcgcgt ctggatcgta
    21001 ggggaggcgg ctgtcttgcc cgtcgatgaa cgtgaacttg gtctggatga acaagtcctg
    21061 gcgcgggatg ccgcttgccg cgatcgcgcg accgacgccg gcctcgaagt agtgcttgcg
    21121 ctgattcgcg gtgtcgatgc cgcgaaagcc cgccgcgagc gcatcacgca cgcagcgctc
    21181 ggtgtcgtcc tctttccatg cagtgccgta gaggaaactc agcgccatgc gcgtttctta
    21241 caacgaacgc agcagtcggc gtagcgggtt gcgacgctga gcgcgcagcg cagctccgga
    21301 ctttgcggtc tcggcgcgta ccgcatgttt cgtatgcgcc gcgcgctcct tgtcagtacc
    21361 gacatagatc tcgcgggtct tggcgaggat gtcgaaccac gaccacacga cgccgaagtt
    21421 cttgtcctga tcgcccatgt ggtgctcgta atgccattgc aggtgatcgc gtccccacgc
    21481 gggatcgaga tgcgcgcggc gatgtacgcg ccgatagcgg tgcaggcagt accagatcgt
    21541 cgacgtgtag aacggcgcga tcggaaacag cggcgcgtgt gcgagcgcga cgatcgcgag
    21601 gccgactgct tcgcgtgctt gcgtcggggt gctccacacc gggccttcgt acgcggggtc
    21661 gtagccgccg ttgcggcgca ccgcctggtg gtgatcgtgg tagtggaacg cgaacgggct
    21721 ccggcgatcg cggcccgcgc cgtgcaagag gtacttgtgc gtggcccact cgccgtagcc
    21781 gaacaccgcg agcgccagcg ggatcccgat cattggtaga aacctagctt tcccccacca
    21841 agcggtgggt ttgaacaagg acccctttca aaccgtgcgt gcagatttcc tgcacacggc
    21901 ttacgggcgg tcgtggttgg acgtgatgag gcattacgca gcctccgggt acgcgacggt
    21961 gccactgagc ttgtgcaatc cgagggtgta gaagtaggac gcgttccagc gatgagactc
    22021 gccaggtttc aagtggcgtc ccttgcgcct gattctcatg cgcttgagcc gccacgcgac
    22081 gtacttgtcg atctcactga acttgtccgc agcgttgccg gtgcggaagt actgccccca
    22141 gccgcggagc acggggttga gctcgccgat cacatcgcga gggtccacgt ggcaccgtga
    22201 cttcgaggtc atgcccttca cgcgccgccg gatccgcttc atgctcctcg tcgatggcca
    22261 gcgctggagg aagtagacgc gcttgcgtcg cttctcccat tgcggccccg agaagcgctt
    22321 ccgcagatgg cagccgagga agtcgatccc ttgcttgccc cagcccagat cgacccgcct
    22381 cgtcttgtcc gggtgtagct ctagcttcaa ctcgcggagg agcttcctca cgcgccgctc
    22441 ggcttctcga cagtccttct ccgtcttgca gagcacgacg aagtcgtcgc agtagcgtac
    22501 cagcacgccg aggtcggcgc accgcttgct ccacacaccg tcgagatagt cgaggtagat
    22561 gttcgacagc agcggcgaga tgactccccc ctgcggcgtg ccgctgagca tcgtcttctc
    22621 gagcccgtct tccatcaccc gtgcttcgag ccactgtctg accagcttca ggactcttcg
    22681 gtccgagatc ttgcggccca ccagcgacaa caacgtctcg tgagagatgt tgccgaaaaa
    22741 gtcgcggatg tccgcgtcca gcacgtggtt gaacccgtgg ttcacggtca cgcggatctt
    22801 ctccagcgcc tgcgtcgtcg agcggccggg ccgaaacccg tacgagcagt ccttgaaccc
    22861 gacctcgaag atcggctcga ggatcagctt cgtcgccgcc tgcacgacgc gatcgcgcac
    22921 cgtcggaatc ccgagcggcc gctgctttcc atcggccttc ggaatgtacc ggcgcaggac
    22981 cgcctgcggc tggtactcgt gctcgcgcaa ctcggcctgg atcgcctcga ggaagcgctc
    23041 cactccgctc tgctcgatcg ccgcgatcgt ctgggcgtcg acgcccgcgg ccccctggtt
    23101 ccgcctcacc tggttccacg cctcgtacag gacgtcactc ctgtagatgc ggtcgtacag
    23161 cgcgtggaac tttcgacccg gctgttgctt ggccacatcc cgcagccgac cttggaggcg
    23221 tcgcacgtcg tcgtcgggtc ttcgcccgcc gggggagttg ggacgcaccg cgtccatgcc
    23281 cttgcgcgta cctcttcgat cgactcgacc atcaccccag ggacccttcc ctccggccgc
    23341 gttgtgctgc acggccttcg tcgctggtgc gggcggtact atgatcccct cggacttccg
    23401 ctgcgcggtg ctcgacttcg ccttgggctt atacgaggca cctctccgtg acggcggctg
    23461 cgcagacggc tctctcgcgt tccgtgacga tccttgcaca cgtgctgcga cccatacccc
    23521 gccgaggccg tcggcgcggt tgtggtcccg ctccgtcggt gttgccttcg ccgtgacatg
    23581 agcggctcgg ccggccggct cacaaccggc gcgctcggat tgtaaatgtg tcgaggctgc
    23641 agccttcact tgctgttgcg gcccgcgtgc ttgcctcctg ccgcgcggct ctcgccgcgg
    23701 gtcaggctct cgacgctccg cttcggacac ggaggtctcc cctcgtgccg ggagcctgct
    23761 actcggcgcc tcacggctta ccgagacggg actctcaccc gctggatcct cacagcgtga
    23821 cgacgggttc gtcctcacct cgtgagggcg gcctccgtcg tcgtcgcgac gcaccatgcg
    23881 gccgttgtag gcggggctct ggcagattga ccagatccgc gtagctgttg agtcaacact
    23941 cttcaacagc tacatgagcg gccacccgag cgcgcggcgc tcgacgaggc ccatgtaaca
    24001 cgtgatcagg tgcccacgta gtcgcgggct cggaaccacc ggagcatcga gacgtgggag
    24061 agcccgaggc ggatggcgcc gccgctcatc gagccggcct gatgtttcgc agcgagccgg
    24121 ag
//
LOCUS       DASUFX010000719         8774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_393108,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000719 DASUFX010000000
VERSION     DASUFX010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393108"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..592)
                     /locus_tag="VFV99_29020"
     CDS             complement(170..592)
                     /locus_tag="VFV99_29020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063467.1"
                     /translation="MIPYDDLVAALSAWRARQGLPVSTLGGGAPGSGPHATPGSGPHG
                     QPGSGPNRGAPPAPPSGRHGAVPPPLEMEADSGMHEVEAEMLEEQYDNEGNDFAMAFG
                     SGDGGDSTAIGGAPERPTDPNDFNGGGRGGRGGRGEDW"
     gene            636..1400
                     /gene="pgeF"
                     /locus_tag="VFV99_29025"
     CDS             636..1400
                     /gene="pgeF"
                     /locus_tag="VFV99_29025"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX5063468.1"
                     /translation="MASAIKLHTSHVLPREGFVHGFPERSGGVSVGLRASLNLGKRWG
                     DDEANVEENRRLLARFAGYDPEQLQATRHVHGTAVWKVGEPAPDPAEFDGLVTDRPGP
                     VLGAFAADCIPILFADPVAKVIGAAHAGWRGTVGGVAGNVITRMVELGAKPADIRVAL
                     GPSIGPECFEVGPEVVEQFRAAFGDVPGMVVRGPKKEHIDLRVASRTVLERAGVVPEH
                     IDDKPPCTKCEADRFFSYRRDGKDGGVHMAFIAMRA"
     gene            complement(1507..2793)
                     /locus_tag="VFV99_29030"
     CDS             complement(1507..2793)
                     /locus_tag="VFV99_29030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063469.1"
                     /translation="MLTEGKSAIDGSTKSLPSGAAFGFATSTLEQARLAKPLSRLLAS
                     KDRKLALDGDEDGFDVDAIIDELNNTLFTDANYLGDGIYRVPPDLVCTDTIYDDTTNT
                     TQEVIDPECAANLAKADLRIRVADDDGLRFYIQLDANHDEPLGILLRHDEIALTVNLD
                     DATDAMIALAQAFGEEAPNADLRGQVTGSIKIHGHAHAGASLSFDRALSIKFADNGVG
                     LDSDGAVRFASAAGEIVAIDLDGNAPKADLDVGLGLTTVHIPGDSGDPSTDVTLGGAT
                     VNATLQNNTLTLDNLSLGNQTTSVAMGGQQAITIDLNPNDGRKLNATVTLDPTTGSET
                     LAVSPRLDLQTSTNHTVLGDEPEVYDVTRVLLDGSLRGSEDGEQVEVVSGSFSITTTP
                     SQYGFSATAGQCVFATEMYDETTFQSWTQYSVGTCL"
     gene            complement(3062..4603)
                     /locus_tag="VFV99_29035"
     CDS             complement(3062..4603)
                     /locus_tag="VFV99_29035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5063470.1"
                     /translation="MRLRAVLFTLLLGSGVAHADLLTRALDDAAAQNAGSSAPSVENN
                     LLSWAGPVRATSLPDLLQVAVRLAPSLQTAKLDIEIAEARIQQSWVRHDWVLTSQLAG
                     SWSDSGVIAGVAIGSSKRLGLTADLTRMIPTGGTVGLHVGTAYDKTETSALTSKTWTD
                     DIGITLTQPILRNAGGFMFNANEQRARISRDVAVLGRRAAALSTVQEIVAAYWDLVLA
                     QRQVAITEQSLTLARERLRITEIGSAGGKIAKAEIPAVQQIIATREEEVLGGELVVLD
                     RSIALRRAVGMEITAGELGLRVATELETQDKGYTVGPMLERAYAASPELAQLAKQEAG
                     STIEIEVNDNGLLPRLDLALQFGPTGTDTTFSGAGKNLVKFDALSAQGTLTFEQSLHR
                     DDVKGRSRELRAGREKLRVNAADLKLQIAQTMAQAIAQIELAKRRVVLSQRAIDLAIE
                     NIRIETDRFNLGKSTNFDVLNRLEEQRQAELRKAQALIDWHKAETVVQALTGDLLPAF
                     GITVE"
     gene            4731..5372
                     /locus_tag="VFV99_29040"
     CDS             4731..5372
                     /locus_tag="VFV99_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063471.1"
                     /translation="MAATVLRTYCFLDALQPQQATFIGKTARGFLPVPYQASLWVEIA
                     PGIAINQVTDAALKATSVQPAVQVVERAYGLLEVHAHDKGEVLSAGATILKHLGVEEN
                     QRLKPRVLTNQIIRGVEAYQTQIINRSSAGMMVLPGQSLFIFECEPAGYAVLAANEAE
                     KAAHVYLVNVTPYGAFGRLYMAGPEAEIDAAAAAATAALNAVTGKEPEKFVDR"
     gene            5401..5697
                     /locus_tag="VFV99_29045"
     CDS             5401..5697
                     /locus_tag="VFV99_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX5063472.1"
                     /translation="MADALGMIEVKGFVGMVEAADAMVKAAKVELVGYEKTGGGYVTA
                     VVRGDVAAVKAATEAGQRAAERVGEVVSVHVIPRPHANVDAVLPLGRSGNGPTK"
     gene            5700..5999
                     /locus_tag="VFV99_29050"
     CDS             5700..5999
                     /locus_tag="VFV99_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX5063473.1"
                     /translation="MVLGKVIGTLVASRKEPTLEGLKLLVVRACDVDGSFASATGALV
                     VAVDAVGAGIGEVVLYASGSSARQTQVTQNRPVDATIMAIVDEVAVDGERRYVKD"
     gene            6003..7454
                     /locus_tag="VFV99_29055"
     CDS             6003..7454
                     /locus_tag="VFV99_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5063474.1"
                     /translation="MAIGGIDERKIEEIVAKVLERLGGSITNAPARGAPIEPAKTKAN
                     IPRGTNGVYQDADQAVKAARKAFEQNERTPIATRAKMVENMRRAVLANNDALSRYAVE
                     ESGLGRYEDKLSKNKLVAEKTPGCEILRPVAYTGDDGLMLTERAPYGVMLGITPTTNP
                     TETILNNAIGMVAAGNAVVFNTHPSAAQTCNWCVHLLNEAIQAAGGPRDVITAVERPT
                     IESANLLMKHPLVRIVVVTGGPAVVKQAMNSGKKAICAGPGNPPAVVDESANLEKAAD
                     AIVRGASLDNNIVCIAEKEVIAVDSIADGLIAQLKRKPVLWLDQRQVKELEKVVLEGD
                     HVNKNWIGKDAALIAKQIGIGGHGHDLRLIMCEVDEQHPFVQHELLMPVIPLFRVRDS
                     ADAIAAALRVEHGFHHTATMHSTNIDHMSAMARVCNCSIFVKNDASYAGLALGGEGYT
                     SFTIASPTGEGLTTAVHFTRERRCTLKEAFRFV"
     gene            7451..8026
                     /locus_tag="VFV99_29060"
     CDS             7451..8026
                     /locus_tag="VFV99_29060"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX5063475.1"
                     /translation="MTATRGPALGVLEVSTIARGVVTADAGLKRAPAVLLHSRAVSGG
                     KHLVFFEGGVAEVEEAMGAGRLIAGDQLLDRVELPAADFQVWLMLGAPIEPLDWSADA
                     GAEAVAIIETKTVCAAIAAADAACKVADVLVRDTRFAVDLAGKAYFTLTGTLDAIEAA
                     AIAATSAANDRLVGLEVIAQPAPDLRGRLFR"
     gene            8110..>8774
                     /locus_tag="VFV99_29065"
     CDS             8110..>8774
                     /locus_tag="VFV99_29065"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX5063476.1"
                     /translation="MSREDKSRLNNMLAGRPGPAGAAATPGKGSNTENLEAGKPLDPS
                     TRSVFESSLSTDLSDVRVHDGEAANAQAGDQDALAYAQGNDIFFAAGQYDPNNDEKRG
                     LLAHEVAHVAQQKGAPADRNPNSTRAGDAAERDADQAAARMVHGAPATVSSTPQQIAK
                     KGPNDKKPTKIPLAQGVTAKFTKIQAGGKPTAAPDGKGGTITFTSPEVEFRGQFSVKS
                     DKV"
BASE COUNT         1453 a         2856 c         3023 g         1442 t
ORIGIN      
        1 gtcgacgcgg ccaacggctg caccgcttgg ctgcatcgct tcgacggcaa tgccgacgag
       61 ctcgagcgcg tgcggttcgt gactcatgca gcgctcgtat cacagctgaa gcaggaactc
      121 tggcttctgc ggcctgaagt ccgaagcccg aggtccgaag cccgacggac taccagtctt
      181 cgccgcgacc gccgcgaccg ccgcgaccgc cgccattgaa atcgttcgga tcggtcgggc
      241 gctccggcgc gccaccgatc gctgtcgagt cgccaccgtc gccactgccg aacgccatcg
      301 cgaagtcgtt gccttcgttg tcgtactgct cttcgagcat ctcggcctcg acctcgtgca
      361 taccgctgtc ggcttccatc tcgagcggcg gcggcaccgc accgtgcctg cccgacggcg
      421 gcgccggcgg tgcaccacga ttcggccccg atcccggctg gccatgcggt cccgagccgg
      481 gcgtcgcgtg cgggccggag cccggcgcac ccccgcccaa cgtgctgacc ggcagacctt
      541 ggcgcgcgcg ccacgcagac aatgcagcaa cgagatcgtc gtacgggatc atggtccacc
      601 gacgatacca cgggccttgt gctaagagct tcgcggtggc ctccgcgatc aagctccaca
      661 catcgcacgt cctcccacgc gagggattcg tacacgggtt cccggagcgc agcggcggcg
      721 tgtcggtcgg gctgcgcgcg tcgctgaacc tgggcaagcg gtggggcgac gacgaggcga
      781 acgtcgagga gaaccggcgg ctgctcgcgc gcttcgccgg ttacgatccg gagcagctgc
      841 aggcgacgcg gcatgtccac ggcaccgcgg tctggaaggt cggcgagccg gcgcccgatc
      901 ccgcagagtt cgacggcctc gtcaccgatc gccccgggcc cgtgctcggc gcgttcgcgg
      961 ccgattgcat tccgatcctg ttcgccgatc cagtcgcgaa ggtgatcggt gcggcgcacg
     1021 cgggctggcg cggcacggtc ggtggcgtcg ccggcaacgt gatcacgcgc atggtcgagc
     1081 tcggcgcgaa gccggccgac attcgcgtcg cgctcggtcc gtcgatcggt cccgagtgct
     1141 tcgaggtcgg ccccgaggtc gtcgagcagt tccgcgctgc gttcggcgac gtgcccggca
     1201 tggtcgtgcg gggcccgaag aaggagcaca tcgacctgcg cgtcgcgtcg cgcacggtcc
     1261 tcgagcgcgc cggcgttgtt cccgagcaca tcgacgacaa gccgccatgt acgaagtgcg
     1321 aagccgaccg gttcttcagc tatcgccgcg acggcaagga cggcggcgtg cacatggcgt
     1381 tcatcgcgat gcgagcgtga cccaggcgac gacgagcgcc gccacgatca gtctcacgcc
     1441 ggcgcggcgc gcgaccgcgc gatgatgatc tttagtcgtg atcgccaccg cgcagtcttt
     1501 tcgcgtctac aggcaggtgc cgaccgagta ctgcgtccac gactggaacg tggtctcgtc
     1561 gtacatctcg gtcgcgaaca cgcactgacc cgcggtcgcc gagaagccgt actgcgacgg
     1621 cgtcgtcgtg atcgagaagc tgcccgacac cacttcgacc tgctcgccgt cctccgagcc
     1681 acgcagcgag ccgtcgagca gcacgcgcgt gacgtcgtag acctcgggct cgtcaccgag
     1741 caccgtgtgg ttcgtcgacg tctggaggtc gaggcgcggc gagaccgcga gcgtctcgct
     1801 gccggtcgtc ggatcgagcg tcaccgtcgc gttcagcttg cggccatcgt tcgggttgag
     1861 atcgatcgtg atcgcttgct gcccgcccat cgcgacgctc gtggtctggt taccgagcga
     1921 gaggttgtcg agcgtgagcg tattgttctg cagcgtcgcg ttcaccgtcg caccgccgag
     1981 cgtgacgtcc gtcgacggat cgcccgagtc gcccggaatg tgcaccgtgg tgaggccaag
     2041 gccgacgtcg aggtctgctt tcggcgcgtt gccatcgaga tcgatcgcga cgatctcgcc
     2101 cgccgccgac gcgaagcgca ccgcgccgtc gctgtcgagg ccgacgccgt tgtccgcgaa
     2161 cttgatcgag agcgcgcgat cgaacgacag cgacgcgccc gcgtgcgcgt ggccgtggat
     2221 cttgatcgag ccggtgacct ggccgcggag gtctgcattc ggcgcttctt cgccgaacgc
     2281 ctgcgcgagc gcgatcatcg cgtcggtcgc gtcgtcgagg ttaacggtga gcgcgatctc
     2341 gtcgtgacgc agcaggatgc cgagcggctc gtcgtggttc gcgtcgagct ggatgtagaa
     2401 gcggaggccg tcgtcgtcgg cgacgcggat ccgcaggtcg gccttcgcga ggttcgccgc
     2461 gcactcgggg tcgatcactt cctgggtcgt gttcgtcgtg tcgtcgtaga tcgtgtctgt
     2521 gcacacgagg tcgggcggca cccgatagat gccgtcgccg aggtaattcg catcggtgaa
     2581 cagcgtgttg ttgagctcgt cgatgatcgc gtcgacatcg aagccgtctt cgtcaccgtc
     2641 gagcgcgagc ttgcggtcct tcgacgcgag caggcgcgag agcggcttgg cgaggcgtgc
     2701 ttgctcgagc gtgctcgtcg cgaatccgaa tgcggcgccg cttggcagcg atttggtact
     2761 gccgtcgatc gcggacttgc cttccgtgag cacgtagcgg aggtcttgcg cgattcgtgc
     2821 acgaacgtcg ctcggcggcg gaggatcatc actgcaggcg atcagagctg ttgaaagtag
     2881 agacaaggaa acaagggctg tgcgcatgaa ggtcgagaca cttcaatggc gatgccagtc
     2941 acccacacat cgcgaaaatc gtcttcgcgc gcgttcatgt ctgtcgattg ccagagcctg
     3001 ccgcgagcgt gtcaacggtc ggccacacgt cagtgttgtc tgacgtcaga tcactggcgg
     3061 gttattcgac agtgatacca aacgcaggga gcaggtcacc ggtcagtgct tgcacgacgg
     3121 tttccgcttt gtgccaatcg atcagcgcct gcgccttgcg cagctcggcc tgccgctgtt
     3181 cttcgaggcg gttcagcacg tcgaagttcg tcgacttgcc gaggttgaag cggtcggtct
     3241 cgatccggat gttctcgatc gcgagatcga tcgcacgctg gctgagcacg acgcggcgct
     3301 ttgcgagctc gatttgcgcg atcgcctgcg ccatcgtctg ggcgatctgc agcttgaggt
     3361 ccgcggcgtt gacgcgaagc ttctcgcggc cggcgcgcag ctcgcgcgag cgacctttga
     3421 cgtcgtcgcg gtggaggctc tgctcgaacg tcagcgtgcc ctgcgcggac agcgcatcga
     3481 acttgacgag gttcttcccg gcgccgctaa acgtggtgtc ggttcccgtc gggccgaact
     3541 gcagcgcgag gtcgaggcgg ggcaacaggc cattgtcgtt gacctcgatc tcgatcgtcg
     3601 agccggcttc ctgcttcgcg agctgggcga gctcggggct cgcggcatac gcacgctcga
     3661 gcatcggccc gacggtgtag cccttgtcct gggtctcgag ctcggtcgcg acgcgcaggc
     3721 cgagctcgcc ggccgtgatc tccatgccga cggcgcgacg cagcgcgatc gagcggtcga
     3781 gcacgacgag ctcgccgccg agcacctctt cttcgcgggt cgcgatgatc tgctggaccg
     3841 cgggaatctc ggccttcgcg atcttgccgc cggccgatcc gatctcggtg atacgcaagc
     3901 gttcgcgcgc gagcgtgagg ctctgctcgg tgatcgcgac ctggcgctgc gcgagcacga
     3961 ggtcccagta cgcggcaacg atctcctgca cggttgacag cgcggcggcg cggcggccga
     4021 gcacggcgac gtcgcgcgag atgcgcgcgc gctgctcgtt cgcgttgaac atgaagccgc
     4081 ccgcgttgcg caggatcggc tgggtcagcg tgatgccgat gtcatcggtc cacgtcttgg
     4141 acgtcagtgc gctcgtctcg gtcttgtcgt aggcggtacc gacgtggagg ccgacggtgc
     4201 cgccggtcgg gatcatgcgg gtcaggtccg cggtcaggcc gagccgcttg ctgctgccga
     4261 tcgcgacgcc ggcgatcacg cccgaatcgc tccacgagcc cgccagctgg ctggtcagga
     4321 cccagtcgtg gcggacccag ctctgctgga tccgcgcctc ggcgatctcg atatcgagct
     4381 tggccgtctg cagtgagggg gcgagccgca ccgccacctg gagcaggtcg ggcagcgagg
     4441 tcgcccggac cggtccagcc caggacagga ggttgttctc gacgctcggc gccgagctgc
     4501 cagcattttg tgctgcggcg tcgtcgagcg cacgggtcag cagatccgcg tgcgcgacac
     4561 ccgaaccgag cagcagcgtg aagagtactg cgcgtagtcg catcgaatcg ggcgcatcat
     4621 ggcatgcacg aaatcgctgc gcatcgtgcg accgcatgcg acgcctgcga cgtttccttg
     4681 ccggtgcgta gatttacaaa aagggcccgt gttagcgtgc gcctctaggg atggcagcca
     4741 cggtacttcg tacgtactgt ttcctcgacg cgctccagcc gcagcaggcg acgttcatcg
     4801 gcaagaccgc gcgcggattc ttgccggtgc cgtatcaggc ctcgctgtgg gtcgagatcg
     4861 cgcccggtat cgcgatcaac caagtcaccg acgcggccct caaggcgacg agcgtgcagc
     4921 cggccgtcca ggtcgtcgag cgcgcctacg gcctgctcga ggtccacgcc cacgacaagg
     4981 gagaagttct ctcagccggg gcgacgatcc tcaagcacct cggggtcgaa gagaaccagc
     5041 gcctcaagcc gcgcgtcctg acgaaccaga tcatccgcgg cgtcgaggcc tatcagacgc
     5101 agatcatcaa ccgcagctcg gcgggcatga tggtgctgcc cggccagtcg ctgttcatct
     5161 tcgagtgcga gccggccggc tacgccgtgc ttgctgcgaa cgaggccgag aaggccgccc
     5221 acgtctacct ggtcaacgtc acgccgtacg gcgcgttcgg ccgcctctac atggcgggac
     5281 cagaagcaga gatcgatgct gctgccgccg cggcgaccgc ggcgctcaac gctgttaccg
     5341 gcaaggaacc cgagaagttc gtcgatcggt agatcgaaac cgctcgcgta ggagaacgag
     5401 atggctgatg cgcttggaat gatcgaagta aaaggtttcg tcggcatggt tgaagccgcc
     5461 gatgcgatgg tcaaggccgc gaaggtcgag ctcgtcggtt acgagaagac tggtggcggt
     5521 tacgtgaccg ccgtcgtgcg cggtgacgtt gctgccgtca aggcggcgac cgaggccggc
     5581 cagcgcgctg cagagcgcgt cggcgaggtc gtctcggtcc acgtcatccc gcgcccgcac
     5641 gccaacgtcg acgcggtgct gccgctcggc cgctccggta acggcccgac gaagtaggca
     5701 tggttctcgg caaggtcatc ggcacgctcg tcgcgagtcg caaagagcca acgctcgaag
     5761 ggctcaagct gctcgtcgtg cgcgcgtgcg acgtcgacgg atcattcgct tcggcgaccg
     5821 gcgcgctcgt ggtcgcggtg gatgccgtcg gcgctggtat cggcgaggtg gtgctttacg
     5881 cttctggctc ctcggcgcgc caaacacaag tgacgcagaa ccgtcccgtc gacgcgacga
     5941 tcatggcgat cgtcgacgag gtcgcggtcg acggcgagcg ccgctacgtg aaggactagc
     6001 ccgtggcgat cggcggcatc gacgaacgca agatcgaaga gatcgtcgcc aaggtgctcg
     6061 agcgcctcgg cggcagcatt acgaacgcgc ccgcgcgcgg cgcaccgatc gagccggcaa
     6121 agacgaaggc gaacattccg cgcggcacga acggcgtcta ccaggatgct gatcaggcgg
     6181 tgaaggcggc gcgcaaggcg ttcgaacaga acgagcgcac gccgatcgcg acgcgcgcaa
     6241 agatggtcga gaacatgcgg cgcgcggtgc tggcgaacaa cgacgcattg tcgcgctacg
     6301 cggtggaaga gagtggcctc ggtcgctacg aggacaagct ctcgaagaac aagctggtcg
     6361 ccgagaagac gcccggctgc gagattctgc gcccggtcgc gtacacgggc gacgacggcc
     6421 tgatgctcac cgagcgtgcg ccgtatggcg tgatgctcgg catcactccg acgacgaatc
     6481 cgaccgagac gatcctcaac aacgcgatcg gcatggtcgc ggccggcaac gcggtcgtgt
     6541 tcaacacgca tccgtcggcg gcgcaaacct gtaactggtg cgtgcacctg ctcaacgagg
     6601 cgattcaagc cgcgggcggt ccacgcgacg tgatcaccgc cgtcgagcga ccgacgatcg
     6661 agagcgcgaa tctcttgatg aagcatccgc tcgtgcgcat cgtcgtcgtg acgggcgggc
     6721 cggccgtcgt caagcaggcg atgaactcgg gcaagaaggc gatctgcgcg ggcccgggca
     6781 atccgccggc cgtcgtcgac gagagcgcga acctcgagaa ggccgccgac gcgatcgtgc
     6841 gcggtgcgtc actcgacaac aacatcgtct gcatcgccga gaaggaagtc atcgcggtcg
     6901 attcgatcgc cgatggcctg atcgcgcagc tcaagcgcaa gccggtgctg tggctcgatc
     6961 agcgccaggt caaagagctg gaaaaagtcg tgctcgaagg cgatcacgtc aacaagaact
     7021 ggatcggcaa agacgccgcg ctgatcgcga agcagatcgg catcggcggt cacggtcacg
     7081 acctgcgctt gatcatgtgc gaggtcgacg agcagcaccc attcgtccag cacgagctgc
     7141 tgatgccggt catcccgctg ttccgcgtgc gcgactcggc ggatgcgatc gcagctgcgt
     7201 tgcgcgtcga gcacggcttt catcacaccg cgacgatgca ctcgacgaac atcgaccata
     7261 tgtcggcgat ggcgcgcgtg tgcaactgct ccatcttcgt caagaacgac gcgtcgtacg
     7321 cgggcctcgc gctcggcggc gaaggctaca cgtcgttcac gatcgcgagt ccgacgggcg
     7381 aggggctcac caccgccgtt cacttcacgc gcgagcggcg ctgcacgctg aaagaggcgt
     7441 tcaggttcgt gtgacggcga cgcgtggtcc cgcgctcggc gtgctcgagg tgtcgacgat
     7501 cgcgcgcggc gtggtgaccg ccgacgccgg gctcaagcgc gcgcccgcgg tgctgctgca
     7561 ctcgcgcgcg gtgtcgggcg gcaagcatct cgtgttcttc gagggcggcg tcgccgaagt
     7621 cgaagaagcg atgggcgcgg gcaggctgat cgcgggcgac cagctgctcg atcgcgtcga
     7681 gttgccggcg gcagacttcc aggtgtggct gatgctcggc gcgccgatcg agccgctcga
     7741 ttggtccgcg gatgcgggtg ccgaggcggt cgcgatcatc gagaccaaga cggtgtgcgc
     7801 ggcgatcgcg gcggccgatg cggcgtgcaa ggtcgccgac gtgctcgtgc gcgatacgcg
     7861 gttcgcggtc gatctcgccg gcaaggcgta cttcacgttg accggcacgc tcgacgcgat
     7921 cgaagccgct gcgatcgcgg cgacgagcgc ggcgaacgat cggctcgtcg gcctcgaggt
     7981 catcgcgcag ccggcgcccg atcttcgcgg tcggttgttc cgctgaccag cgtaacgtcc
     8041 cgaattcgct ggcccgttca tgacggctga ccaggcaggt ttcgcctggg ttcgccgtaa
     8101 gataggagcg tgtcgcggga agacaaatct cggctgaaca acatgctcgc gggccgtccg
     8161 gggccagccg gtgctgccgc gacgccgggt aaggggtcga acaccgagaa cctcgaagcc
     8221 ggcaagccgc tcgatccgag cacgcgctcg gtgttcgaat cgtcactgag cacggacctg
     8281 tctgatgtcc gggtccacga tggcgaagcg gcgaacgcgc aggcgggcga tcaggatgcg
     8341 ctcgcgtacg cgcaaggcaa cgacatcttc ttcgcggccg gccaatacga tccgaacaac
     8401 gacgagaagc gtggcttgct cgcgcacgaa gtcgcgcacg tcgcccagca gaagggcgcg
     8461 ccggccgacc gcaatccgaa ctcgacgcgt gcgggcgatg ccgccgagcg cgacgccgat
     8521 caagccgccg cgcgcatggt tcacggcgca ccggcgacag tcagctcgac gccgcagcag
     8581 atcgcgaaga agggcccgaa cgacaagaag ccgacgaaga ttccgctcgc gcaaggcgtg
     8641 accgcgaagt tcacgaagat ccaggccggc ggcaaaccga ccgccgcgcc cgacggcaaa
     8701 ggcgggacga tcacgttcac atcaccggag gtcgagttcc gcggccagtt ctcggtgaag
     8761 tccgacaagg tcga
//
LOCUS       DASUFX010000720        25711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_670802,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000720 DASUFX010000000
VERSION     DASUFX010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25711
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670802"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFV99_29070"
     CDS             complement(<1..1051)
                     /locus_tag="VFV99_29070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5063477.1"
                     /translation="MSKNRVLAGIAAGKPQTGPRTVHVDVTNACNAACITCWDHSPLL
                     VEQRSAAWKKRRIDRAVFDELVTDLATLGSVKAMILSGMGDPLVHPDIYAMIERVKRE
                     GWHLTVMTNLIAADIERLADLGVDQFLVGVHGATPVAYSAFHPGWDEREFFTLCKYLR
                     RLGSTTSNVRHVQVINRDTAPEFVGMVRFGHQFRAERVNFKLASLAGGTESCRITDEQ
                     RRWLAAEAIPQAHALATQLGVTTNLDLFETQLAAGVEDEQATVPIADVGCFMGYVYTR
                     ITASLDVLYCCNTEVKVGSLADARLSELWRGDAWQALRDRLRRGDYFAGCERCGKFEQ
                     NVEWSKRVKSELGDAV"
     gene            complement(1048..2649)
                     /locus_tag="VFV99_29075"
     CDS             complement(1048..2649)
                     /locus_tag="VFV99_29075"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5063478.1"
                     /translation="MKRLRIVSYAINGRGLGHLVRQLSILRWVRRYGALLGVNTECWV
                     LTTSEADTLARREGIPAFKLPSKAMMRDAAIDPTRWLAVGRQWVMNAIAGLSPDVLVV
                     DTFPGGSFGELLPVLETVPTRVLVARAVKDEIAADDGYRALLPLYQKAIVPDERGVGP
                     ILLREREELMPRAEARSALGVSCDRAVYVTLGGGGDEAAARVLPVLTDKLVARGWHVV
                     VGAGPLYIGPERRGPHITWMDRYVPLELMQGVDAAVSAGGYNSVTELMFAGVPTVFLP
                     QPRIADDQDGRAKAAAAAGAARLANSIDEVPDLLESPGDPAAARALVPHNGARAAAAE
                     ILSTVLPAADIAMAQSVVTPELWARAQRLGGDKALEAVSVLVGDAPSAQKERDALLAE
                     LVAEGALISAPARRRMPAAVRVEKFLAAVDQAQAPIDTAIAIVRGLKRKFPAAGLDAL
                     VDACVVLFPAWARFADWMGAVSLVNALPLQRGVPVGEIAAAIVIWLERETDLFDALRR
                     FAHLEGNGARSLGDVLRALPNEVEA"
     gene            complement(2646..3965)
                     /locus_tag="VFV99_29080"
     CDS             complement(2646..3965)
                     /locus_tag="VFV99_29080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063479.1"
                     /translation="MSVSGQQVADHYKDQASTLAGLIAQHKRLLDTFKARDQELSNQL
                     LEARRELASVYLKTLDDTNLEFVAQLTGFQGFLRRDPRVAREQERKVLQSSIGKLEAD
                     ERYQKRDVLAGPDGTLTQELNQNKETLAPLQAECDRFESLQDFAELIAVGYDTPQFQE
                     KWWHATYWKHWAAGDKICRELGMKDFGDDVIPAYNKVAEPRNFMRGEVGRLDKEITAI
                     HELVQQHDQMADRLARLDEIYLTEAQEFLGEHLMNADAALLEQWAQDKPEILRGVQMG
                     VRKIAGITAKRSILVDMAQSGVPQTIQQLEQRQYKAYSKFNKFSRPKYAYSSFSNDMV
                     NDGFAEKSQNMRTQIDKLERRVDTLMANQNYAGFDLRNDPYLWWWYFMESAPPRYAPH
                     YYEYYQRNPSFNVVTDPDFVDMAPDRSEDVARAFVASDAETGGGILS"
     gene            complement(4002..5303)
                     /locus_tag="VFV99_29085"
     CDS             complement(4002..5303)
                     /locus_tag="VFV99_29085"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5063480.1"
                     /translation="MSTSIERIITARTRDLGGFAVGRVLPTVGRRFVGPFVFFDHMIR
                     AEVGELAVRPHPHVNLGTVTYLFEGEVMHRDSLGSKQLITPGAINWMSAGHGIVHSER
                     TPPGAEVRPHHGIQLWVGLPREKEESAPSFKHYPAPTLPIVDETGMKARVLIGNAFGT
                     ASPVETFWPMFYVDVALEPGGRVALPAGYRERAVYVVDGALLADGQRIEPHKMALFAA
                     NADPVLEADGPARLLLLGGEPLDGPRYIWWNFVSSSPDRIIEAAHAWREDKFPKIAED
                     NVEFIPAPDDDPRFALSYHVPSDDELRRVLIHAKTIAMVGASNNPQKPSHRIFKQLLN
                     AGYHMIPVNPNEAEVLGQPAVKQLSDIKEPVDIVDVFRKSEDAPPIAEDAVKLGAKAL
                     WLQLGIASEEAALRALKGGLTVVMDTCIGATHHRLQIPPKS"
     gene            complement(5300..6172)
                     /locus_tag="VFV99_29090"
     CDS             complement(5300..6172)
                     /locus_tag="VFV99_29090"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5063481.1"
                     /translation="MNRNVKQLVTAHRQREGAGFVVRRPVPTMGLELVDPFLLIDELG
                     PVTYGPGEAVGAPDHPHRGFETVSYILEGAVEHEDSAGHRGVINAGDVQWMTAGGGII
                     HSEMPPRELLEKGGRVHGFQIWVNLPRKLKLTTPRYQELPAAKIPEAKTADALARAKI
                     IAGAALGVNAAIETHTPIVFQDWTLEPGADVTIDISKDQQVLAYVFEGAIEIEGREVR
                     DGQMAILGDGDAVRLRGGAQRGRLLLLAGVPHREPVARYGPFVMTSERELIEAVHDYQ
                     SGRMGEITRTARVG"
     gene            complement(6253..6768)
                     /locus_tag="VFV99_29095"
     CDS             complement(6253..6768)
                     /locus_tag="VFV99_29095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5063482.1"
                     /translation="MAKAKAKAKPAAKKSASKKPAKAKPAGRSLSKSKAAAKTAAKKP
                     AKKTAAAPVGPYLRSLRTCIYQVHDLPLAKEFYSQALGKDPYFDEPYYVGFDVDGHEL
                     GLDPDTSKRPPGGSGVIGYWRVEDINAAWQHLTTIGARSIELPHSVGVDLQVSIVSDP
                     FGNYIGLIQGA"
     gene            complement(6777..7937)
                     /locus_tag="VFV99_29100"
     CDS             complement(6777..7937)
                     /locus_tag="VFV99_29100"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX5063483.1"
                     /translation="MTKSEGFGTRAIHAGQEPDPVTGAVMTPIYYTSTYAQTAPGEHK
                     GYEYSRTHNRTRYALEANLASLEGGVGGLCFASGLAATATLLDAFDAGTHVIACDDLY
                     GGTFRLFDKVFRRLGFEFTYVDPLGGAAAVEAAIKPNTKLVWIETPTNPMLKLVDIAA
                     VAVVCKAKGLMLAVDNTFMTPYFQRPLELGADVVAHSMTKYLNGHSDVVGGALIFKDQ
                     ALRDRVAFLQNAVGAVISPMDSFMVLRGTKTLHVRMDRHEQNARQLAAWLDKHPQVER
                     VIYPGLESHPQHALAKKQQRGFGGMISFVLEGNLDHARRFLSTCKLFTLAESLGGVES
                     LIEHPAIMTHASVPPENRKKLGIVDGFIRLSVGIEDLADLQADLDRAFAAAK"
     gene            complement(7934..8419)
                     /locus_tag="VFV99_29105"
     CDS             complement(7934..8419)
                     /locus_tag="VFV99_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5063484.1"
                     /translation="MNPAEVATALLIERTLQDSPAYRKIDDSLYVIKQGSAYVMISVV
                     PWGDNKAMVRCTSQLVKGVNVDGPLAKQLLELNAHLRFGAFAWEPGEQTVLFTHTILG
                     GTTLDPEELMATLRDVALIADEYDDKLMKKYGGQRMIDLLEEAAMERIMEKDPKGFQF
                     E"
     gene            8461..10167
                     /locus_tag="VFV99_29110"
     CDS             8461..10167
                     /locus_tag="VFV99_29110"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5063485.1"
                     /translation="MRRVALALLITASTAARAQTPYPVPVPPEELPPSPPPAPPPDPR
                     PTPVPLGPDNKPIEQQPQQPAPQQPQQPPQQQPTQQQPQPQQPQPQPPQPQPPQPQPP
                     QQPQPQPTPPPPDLKIEQKLEPNETLPTAADLTASLTIKKIVVEDNTKTTSDTVEVLA
                     GIETGDSWNASMVDVVKTRLVSSGLFKDVEVYWTRIPEGVVVHILAKDKHSWVIAPAF
                     YNQPTNVGGGIGFGENNLFGQNQKLLLYGQIATGDSFFIGAWVIPSIGGTRFYSQLDT
                     YLKSGRNIEYTAPDSWLVEKDEIRPIRESRIHYLNFGVKLGIELWRGVKVDTRLRAAS
                     VSYQDVHLSDGATMADIKPGPDPMVVPAPGMEGTDVSNEWSFTIDRRANWYGVQEGRK
                     YTVSYEHATPSLGSDFRYWLASFSAFRAYKVLDRHNLILKGAVNYGHNLPFQQEFQTG
                     GTSMRGWLNNEFRGDFRTLANLEYSVPMFTIHGFSVRGLAFWDSAYTTFINTTDNETD
                     GVRNYLPNSEVRGLDGFKNSVGVGTRFYLRQIVLPLLGLDFGYGLEARDVQIYLAIGL
                     TD"
     gene            10238..11776
                     /locus_tag="VFV99_29115"
     CDS             10238..11776
                     /locus_tag="VFV99_29115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5063486.1"
                     /translation="MLLHGDVFTPTGHKSAVAVRGGDIVAYDDAEKWIGGKTRVLDLA
                     GKSVIVGLTDAHCHLYGLGVDMENVSVRALGSEAEVVKVISDAAKTRPASEWLIGRGW
                     DQNRWAGQQFPTKATLDAAVSDRPVLLRRIDGHAIWVNSRALAEAAITKATKDPAGGK
                     IVRDANGEPTGVLIDNATDLVDAKVPAATADVRERRILAAAKVATELGFTAVHEMGIE
                     DATADVYRKLAAANQLPLRVYAYLAGDAAHADELRKRPPAPATGRFVMRGVKYFVDGA
                     LGSRGARLYEEYDDDKANLGLWVTEPKQLAAAVDASVAGGWQTAIHAIGDAGIGAVLD
                     AYLAAQAKHPGDHRLRVEHTQVIAMKDVPRMVQAHAVASMQPTHATSDMPWAEARVGK
                     QRIKGAYAWRTMLNEKIPLAFGSDFPVEEVNPLLGLDSAVNRGNWYPEQKLTLDEAVA
                     AFTTGAAYAVHEEAQAGRRADLTVFDAKLTPTDLPKRNVLMTMVGGEIVFERQGALAE
                     ATGR"
     gene            11776..13683
                     /locus_tag="VFV99_29120"
     CDS             11776..13683
                     /locus_tag="VFV99_29120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063487.1"
                     /translation="MRFCPFCSAENADESAVCSACGRRLPPLPPRRAGSKGPPTGVQL
                     PPRASSPPPPRKPTTLPPPIPSAGVPTETAFRDSAPALPPPPPSFEDQATEVAAELRN
                     AIDPGPAAQRREAPPIEAPTAGAPARRKSDSKAPPLPPTMARSGGSGAIGTPTPTPVR
                     GPGSGSLPASVAERAGSHHDTTYERSKSPASPLERSKSPSQPPPFDRSKSPSQPPPFD
                     RAKTPSRPPLPRPGSSGFGGGETMVSPPPSFETEPAASAYPLQVAANRLRASEIEPPP
                     TRIQRSDATDRPFTPQKVNAVPEIPEPGLVNAARYAYQFALARWQRRGAIKTLGIEIK
                     QDTEALDQVLGALGRAARGARVEGRVFSAENAAISAAEERVGSLQKESGDVEGRKAEE
                     NSKFVDVERERNTKLVEAERQVEEAQRELQHLEAQRRSLRDKRKELDRRQKAYLKNAD
                     DHDRQAGAAPLGDQRQELRRAAEGHRKEAAALEPDKQDIDRRITALERPISEATAKLD
                     AAKAELDASKRSLNDVREGHTHRLAELDAEQKRKAREVALAEAEIQRRLVTLGTLVNL
                     NRIEDPQFAELYQRIDRLRGAITARTTEIEKLTAEREAYDRGTLVRGVATIGGAFVAL
                     IALIVILRAVL"
     gene            13702..14799
                     /locus_tag="VFV99_29125"
     CDS             13702..14799
                     /locus_tag="VFV99_29125"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5063488.1"
                     /translation="MPKVLVSNNSELLRHFTAAPFQRLGLQLLVAETATEATAMFMKE
                     EPPLVVLDAETEGFSIAKKCKAHNPGTRVILVAGKRLNGDQMREVSASGCDELLIAPM
                     TADELHDVIAIQLGEPRPGTEAFAVTVQLGGKKVDATVSNLSIDGVRVVVLEPVSEGQ
                     AVEVTITPDGESPIAIKGTAVWAQPRDSKTVVGVAFNELDAKAKTMLAKLTQWQVVKD
                     GERTRVVLRGDFTEATRFDELLPAMVGRIVFDMAQVTYMNSLGVRAWCEFLREARIQG
                     YEFHACSIPFVLQASMVRDVIGRGTVTSFFAPFHCISCDHQEERLLQSAAILASNLEP
                     PVFKCPNCGGALEFDDLPERYFAFLADNAAE"
     gene            14866..16362
                     /locus_tag="VFV99_29130"
     CDS             14866..16362
                     /locus_tag="VFV99_29130"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HEX5063489.1"
                     /translation="MRKLFVRSLVIAFVAFALLFGGEFLLEWRRAGFPVWGAQSWVGF
                     NNAKLVDMLSPMARAYNNVLAMLIATIGLAIPLTANMHTPKLIEMFLKDRVNRWVLSF
                     IALGAAHVLWVEYMIGPQFAPTWAITVAIWLAIAGWVVLLPYFFYVVRFVDPSRLLVR
                     LRDGTTFVVGKVADREFDPYEAQLDVSTRINQLGTIIIKSLDRNDRDVAAEGTWAIKR
                     LLDDYDKHKSRMPKEWFKVDRADFVGLSDEALEMLSENRTWFEMKCLQQIEHGFLRAL
                     HGANDTVSTFSDATRVIACRADAHHDEQALRLCIRFFNNYLREAIKARNLRAVYDVFH
                     QYRRLGRDLVDRPELLREIGNHFAYYAVMARTYGMLFAPQLALFDLGYVTRRAYERAS
                     PAAPDLLKTVLALPHRTGNDLHTMAVKAKLILGGFFVENRLEAEADLVRKNLNDVDSV
                     SVERAEAELLAAERSFFEVTDRQLNLEWVPPERREPLKQFCNSLHNGA"
     gene            16412..16693
                     /locus_tag="VFV99_29135"
     CDS             16412..16693
                     /locus_tag="VFV99_29135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063490.1"
                     /translation="MRVVEVVIAAVLASACAPHTQYAASALSDSNSEHMGICDVVEQC
                     SVGGRGSNLGLAIAVGSAVAGVLTLALVRHIALDFRSPQRSVTAAPKRN"
     gene            complement(16690..17124)
                     /locus_tag="VFV99_29140"
     CDS             complement(16690..17124)
                     /locus_tag="VFV99_29140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063491.1"
                     /translation="MSEADEDIDDEIVAKVSDYVDGKLTAAERAEVAKKIETDPVWKQ
                     THDDMIENEKFMSGMQKARPVPAPASFTNDVTDTIHKRSAGRFFGRKTFGDRVPFNAI
                     LIVAIIALATVGYFMWASQTGSLKVEKKPTTNEPTAKPPVKL"
     gene            complement(17117..17707)
                     /locus_tag="VFV99_29145"
     CDS             complement(17117..17707)
                     /locus_tag="VFV99_29145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5063492.1"
                     /translation="MSDLDPALLRRLLDRDERAFRHLVEQHRDKVFNITFRMLGNRAE
                     AEDVAQEVFISVFKSIEQYREEAKFSTWLYRIAVNHCKNRIKYLARRHNRDQEEIDES
                     THGPGINGSIGAPLPSSPHKALESAQAEKLMREAIASLDEDQRIVVVLRDVEDMSIEE
                     ICAITELPDGTVKSRLHRARLVLRKKLQRHLGDQHE"
     gene            18047..18499
                     /locus_tag="VFV99_29150"
     CDS             18047..18499
                     /locus_tag="VFV99_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5063493.1"
                     /translation="MNTRLYVGNLSFNTTADGVRTAFQQFGNVSDVHLVTDRETGRSR
                     GFAFVTMGTTEEAAKAIEGMDGKTLDGRPLRVNEAEQRQARGGGGGGGGFRGGGGGGR
                     GGYGGGRGGDDYGGGGGGGGGDWGGGGDDRGGRGGRGGRGRGGGGDRW"
     gene            complement(18649..19560)
                     /locus_tag="VFV99_29155"
     CDS             complement(18649..19560)
                     /locus_tag="VFV99_29155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063494.1"
                     /translation="MRLVRTSLIMVISLMGSAYAEGDALVDNLGPREIAVGEAMRGAA
                     TGASAIGLNPAGLPLNRELVFEGGYGYRLSDSASLVGVSACDSTGAYPGCFYYQYSGS
                     SPELGGMAMSRRTHLGGMSLAYPVTPRVFIGSSVKYFNFDSDLASEMKASGFNWDLGA
                     TLRISELVNIGAAGYNLWGADSPEFPRAVGGGVLARPMPLLALSFDARWKLEDNDHTA
                     RYGGGAELFLRPGDGQAGFPIRAGVLHDNGMDATYLSAGLGYATMKLAVDVAARYAVS
                     GAEATMFIASLRFFGPRQAAPALDVAE"
     gene            19633..20142
                     /locus_tag="VFV99_29160"
     CDS             19633..20142
                     /locus_tag="VFV99_29160"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5063495.1"
                     /translation="MKDTIDALELLKEQHDEVEDLITQIEDSDDPDDKEELFQKLADR
                     IAAHSTIEEKLFYPSVMSEETQELLLESTEEHLAVKRVLADMLDMDADDERFDAKLTV
                     LKESIRHHARDEEEGMLFPILRRRLSAETLAGLGNELLAFFEKLLEREPRMNVPAETN
                     AAAPLELGM"
     gene            complement(20149..20673)
                     /locus_tag="VFV99_29165"
     CDS             complement(20149..20673)
                     /locus_tag="VFV99_29165"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX5063496.1"
                     /translation="MSVEQLFGEGRDLSALQMSSRAFVLFFLMLVLVRVAGMRAFGRH
                     SSFDTIIVITLGAVLSRVVVGVSPAIPTVAASIVFVVLHRLVAIVTATVPALERLFKG
                     RQKVLYRGGILELAAMRRAGISHADLEEAVRTKANRLKLPDVLEIHLESSGDLSVVDD
                     VIGEARRVRRATAA"
     gene            20733..21977
                     /gene="egtB"
                     /locus_tag="VFV99_29170"
     CDS             20733..21977
                     /gene="egtB"
                     /locus_tag="VFV99_29170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX5063497.1"
                     /translation="MSASSSLSTRYRSVRQRSLSVCAPLSAEDAQVQSMPDVSPAKWH
                     LAHTTWFFERFVLARVGDSPLDPAFEYLFNSYYDSVGAHVDRARRGHLTRPALPVVHA
                     YRYAIDTRIEEVLADKPDVETLARIELGLHHEQQHQELMLTDLKHVLGMQPLRPAYRP
                     DLARPANATVEMRHWIEIDGGVVEVGAKGAGFAYDCERPRHCVLLSSYRLASRPLCNA
                     EVLAFIADGGYRDSRFWLADGFALARSENWRAPLYWEQRDDVWLHYTLAGLREVDPAE
                     TACHLSYYEADAIARWAGARLPTEAEWEHAAARVELTGNFADDDALHPSTASGDGLRQ
                     LFGDVWEWTQSAFAPYPGFRPLDGALGEYNGKFMANQLVLRGGSCLTPRDHMRASYRN
                     FFAPSARWQMTGVRLALDGEQS"
     gene            21974..22921
                     /gene="egtD"
                     /locus_tag="VFV99_29175"
     CDS             21974..22921
                     /gene="egtD"
                     /locus_tag="VFV99_29175"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX5063498.1"
                     /translation="MNIAARAPIPQDDILTGLLSRPKRLPCRLLYDRRGAELFDRITC
                     LDEYYPTRVELRLLDECLPKIAANVGVAARVIEPGSGAGIKTKRLLRALDQAASYVGI
                     DVSRESLAYTAEVLSREHPRLDIHTIEGDFTRPFALPTPRRPVGRTLVFFPGSTIGNF
                     EPHEAVMFLSGLLRGVGANSRLLVGADGTREREVLEHAYNDYEGVTAEFNKNVLAHLN
                     RTRGTAFDLDAFDHRAIWSERLSRIEMHLIAKRPQHVILDGTAIVLAPGESIITEYSY
                     KHTLTAMRSILLAAGWRVSDVYTGKEQPMRLWLCEPAQE"
     gene            22933..23493
                     /locus_tag="VFV99_29180"
     CDS             22933..23493
                     /locus_tag="VFV99_29180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063499.1"
                     /translation="MAKSDSPLVQAATQFDEELAIYSRLGELFLKTPLTSLKQLERAN
                     QILGEIADCEQRLSDAGKRLIEALTGARQTQEGLSQSVVAHAPTVQARNTKLRELMAA
                     MAALASDVQGVNALVLQKNGDQQQDAAKPDPGDVSGKVLELSERAEALATSCREAEFA
                     ELAEQAHSLHQRLKAIGTKLQKAAGN"
     gene            23620..23985
                     /locus_tag="VFV99_29185"
     CDS             23620..23985
                     /locus_tag="VFV99_29185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063500.1"
                     /translation="MNSLKLSELSIDVVETPESTFQCTWRGKSYERNAADTLSAWLDA
                     LLATAVAKGGAIEMHFEDIEHFNASTITVLVRHIQACRAQKVKLTMVYNGALNWQSLS
                     FDALRIFVMRDDLFTLRPA"
     gene            complement(24355..>25711)
                     /locus_tag="VFV99_29190"
     CDS             complement(24355..>25711)
                     /locus_tag="VFV99_29190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063501.1"
                     /translation="DLTWGPSWVNWRAGNGWIGWSPAGYDNDAYVPEDSWRWLHAPDL
                     FAVDINRRFVTNNYKVYLSTSQPILRYNKFKKFQWVAGPSEDWFRRYNVYFRKDRSDP
                     SELGRFEAQRKAEADRRARERKKEFDERRKREEQVRKQLEEQQRRQKQEEQRLADEQK
                     RQAKERERQEKERQRLEEQRKKFEEQQARARNDAERKRLEDERRKIEEQRKKLDDERR
                     RAAEAEAKRMQDEQRRRDEEQRKAKEAQERQRAEQQKRFEEQQRKQKEEEAKRAAELE
                     KRRQEDAKRLEEQKRKAEQEQRKRDEEAKRKAEEDARKKAAEDQRRQQEEKKRADQEA
                     KRLEQEKRKQEEEQRRQQEQDAKKAEQEKRKQEEEQRKQDEARKRQEEQDRKKAEQDA
                     KRQQDEQRKQDEARKKQDEENRKKAEADEKKKQDEQKKQEGDKKQQDDKKKGNDKGGK
                     K"
BASE COUNT         4300 a         8590 c         8264 g         4557 t
ORIGIN      
        1 agaccgcgtc gccgagctcg ctcttcacgc gcttgctcca ctcgacgttc tgctcgaact
       61 tgccgcagcg ctcgcaaccc gcgaagtagt cgccgcgccg cagccggtct cgcagtgcct
      121 gccatgcatc gccgcgccac aactcggaca gccgcgcgtc ggcgagcgag ccgaccttga
      181 cctcggtgtt gcagcaatag agcacgtcga gcgacgccgt gatccgcgtg tacacgtagc
      241 ccatgaagca gccgacgtcg gcgatcggga cggtcgcttg ctcgtcctcg acgccagccg
      301 cgagctgggt ctcgaacaga tcgaggttcg tcgtgacgcc gagctgggtc gcgagcgcgt
      361 gcgcctgtgg gatcgcctcg gccgctagcc agcgccgttg ctcgtcggtg atccggcagc
      421 tctcggtgcc gcccgcgagg ctcgcgagct tgaagttgac gcgctcggcg cggaactgat
      481 gcccgaaccg caccatgccg acgaactcgg gcgcggtatc gcggttgatc acctggacgt
      541 gacgcacgtt gctcgtcgtc gagccgagcc ggcggaggta cttgcacagc gtgaagaact
      601 cgcgctcgtc ccagcccgga tgaaacgcgg agtacgcgac cggcgtcgcg ccgtggacac
      661 cgacgaggaa ctgatcgacg ccaagatccg cgagtcgctc tatgtcggcc gcgatcaggt
      721 tcgtcatcac cgtgaggtgc cagccttcgc gcttgacgcg ctcgatcatc gcgtagatgt
      781 ctgggtggac gagcgggtcc cccatccccg acaggatcat tgccttgacc gagccgagcg
      841 tggcgagatc ggtgacgagc tcgtcgaata cggcgcggtc gatgcggcgc ttcttccacg
      901 cggccgagcg ctgctcgacg agcaggggcg agtgatccca gcacgtgatg caggcggcgt
      961 tgcacgcgtt cgtgacgtcg acgtgcacgg tgcgcgggcc ggtctgcggc ttgcccgctg
     1021 cgatccccgc gaggacccgg ttcttgctca cgcctcgacc tcgttgggta gcgcgcgcag
     1081 cacgtcgccg agcgaccgcg cgccgttgcc ttcgagatgc gcgaacctgc gcagcgcgtc
     1141 gaacagatcg gtctcgcgct cgagccagat gacaatggcg gccgcgatct cgccgacggg
     1201 cacgccgcgc tgcagcggca atgcgttgac gagcgacacc gcgcccatcc aatcggcgaa
     1261 ccgcgcccat gcggggaaca gcacgacgca cgcgtcgacg agcgcgtcga ggccagccgc
     1321 cgggaacttg cgcttgaggc cgcgcacgat cgcgattgcg gtatcgatcg gcgcttgcgc
     1381 ctggtcgacc gcggcgagga acttctcgac gcgcacggcc gccggcatcc tgcgccgcgc
     1441 gggtgccgag atcagcgcgc cttccgccac gagctcggcg agcaatgcat cgcgctcctt
     1501 ctgggcactc ggcgcatcac cgacgagcac actcaccgct tcgagtgctt tgtcaccgcc
     1561 gagccgctgc gcgcgtgccc acagctccgg cgtgaccacg ctctgcgcca tcgcgatgtc
     1621 ggcggcaggt agcacggtcg acaggatctc cgcggccgct gcacgcgccc cgttgtgcgg
     1681 tacgagcgcg cgagccgctg ccggatcgcc cggcgattcg agcaggtcgg gcacctcgtc
     1741 gatcgaattc gcgagccgcg ccgcaccggc agccgcggcc gccttcgcgc ggccatcttg
     1801 atcgtcggcg atccggggtt gcggcaggaa caccgtcggc acgccggcga acatcagctc
     1861 ggtcacgctg ttgtagccgc cggcggatac ggccgcatcg acgccttgca tcagctcgag
     1921 cggcacgtag cgatccatcc acgtgatgtg cggaccgcgc cgctcgggtc cgatgtagag
     1981 cggaccggca ccgaccacga cgtgccagcc ccgcgcgacg agcttgtcgg tcagcacggg
     2041 aagtacgcgt gccgctgctt cgtcaccgcc gccaccgagc gtcacgtaga ccgcgcggtc
     2101 gcacgacaca ccgagcgcgc tgcgcgcctc cgcacgcggc atcagctctt cgcgctcgcg
     2161 gagcaggatc ggcccgacac cgcgctcgtc gggcacgatc gccttctgat acagcggtaa
     2221 caatgcgcga tagccgtcgt cggccgcaat ctcgtccttg accgcacgcg cgaccagcac
     2281 gcgggtcggc acggtctcga gcacgggcaa gagctcgccg aagctgccgc ccgggaacgt
     2341 atcgacgacg agcacgtcgg gcgaaaggcc cgcgatcgcg ttcatcaccc actggcgccc
     2401 gacggcgagc cagcgcgtcg gatcgatcgc ggcgtcgcgc atcattgcct tcgacggcag
     2461 cttgaacgcc gggatgcctt cgcgtcgtgc aagcgtgtcc gcctccgacg tcgtgagcac
     2521 ccagcactcg gtgttgacgc caagcagcgc gccgtagcga cgcacccagc gcaggatcga
     2581 gagctggcgc acgagatggc cgagcccgcg gccgttgatc gcgtagctga cgatacgcag
     2641 tcgcttcacg acagaatccc tcctccggtc tcggcatcac tcgcgacgaa tgcccgcgcg
     2701 acgtcttcgc tgcgatccgg cgccatgtcg acgaagtccg gatcggtgac gacgttgaag
     2761 ctcggattgc gctggtaata ctcgtaatag tggggcgcgt agcgcggcgg cgccgactcc
     2821 atgaagtacc accaccacag atacggatcg ttgcgcagat cgaagccagc gtagttctgg
     2881 ttcgccatca acgtgtcgac gcggcgctcg agcttgtcga tctgcgtccg catgttctgc
     2941 gacttctcgg cgaagccatc gttgaccatg tcgttcgaga agctcgagta cgcgtacttc
     3001 ggtcgcgaga acttgttgaa cttcgaatac gccttgtact ggcgttgctc gagctgctgg
     3061 atcgtttgcg gcacgcccga ctgcgccata tcgacgagga tcgagcgctt tgcggtgatg
     3121 ccggcgatct tgcgcacgcc catctgcacg ccgcgcagga tctccggctt gtcctgcgcc
     3181 cactgctcga gcagcgccgc gtccgcgttc atcagatgct cgccaaggaa ctcctgcgcc
     3241 tcggtcaggt aaatctcgtc gagccgcgcc agccgatccg ccatctgatc gtgctgctgc
     3301 acgagctcgt ggatcgcggt gatctccttg tcgagccgac cgacctcgcc gcgcatgaag
     3361 tttcgcggct cggcgacctt gttgtaggca ggaatgacgt cgtcgccgaa atccttcatg
     3421 ccgagctcgc ggcagatctt gtcgcccgcc gcccagtgct tccagtaggt cgcgtgccac
     3481 cacttctcct ggaactgcgg cgtgtcatag ccgaccgcga tcagctcggc gaagtcttgc
     3541 agcgactcga accggtcgca ctccgcctgc aacggcgcga gcgtctcctt gttctgattg
     3601 agctcctgcg tgagcgtgcc gtcgggaccg gcgagcacgt cccgcttctg gtagcgctcg
     3661 tcggcttcga gcttgccgat gctcgactgc agcaccttgc gctcctgctc gcgcgcgacc
     3721 cgcggatcgc ggcgcaagaa gccctgaaag cctgtgagct gcgcgacgaa ttcgaggttc
     3781 gtgtcgtcga gcgtcttcaa gtagacactc gcgagctcgc gacgcgcctc gagcagctga
     3841 ttgctcagct cctgatcgcg cgccttgaac gtatcgagca gccgcttgtg ctgagcgatg
     3901 agcccagcca gcgtgctcgc ctggtccttg taatgatcgg ctacctgttg ccccgaaacc
     3961 gacattcgcc gcgtagtttc gccacgcgcg gcatcgctcg tctacgactt gggcgggatt
     4021 tgcagccgat gatgcgtcgc gccgatgcag gtatccatca cgaccgtgag cccgcccttg
     4081 agcgcgcgaa gagccgcctc ctcgctcgcg atgccgagct gcaaccacag cgccttcgcg
     4141 ccaagcttga ccgcgtcctc ggcaatcggc ggcgcgtcct ccgacttgcg gaacacgtcg
     4201 acgatgtcga ccggctcctt gatatcggag agctgcttca ccgcgggctg accgagcacc
     4261 tcggcctcgt tcggattcac cggaatcatg tgatagccgg cgttcagtag ctgcttgaag
     4321 atgcggtggg acggcttctg cgggttgttc gacgcgccga ccatcgcgat cgtcttcgca
     4381 tggatcaata cgcggcgcag ctcgtcgtcg ctcggcacgt gatagctgag cgcgaaacgg
     4441 ggatcgtcgt cgggcgcggg aatgaactcc acgttgtcct cggcaatctt tgggaacttg
     4501 tcctcgcgcc acgcgtgcgc ggcctcgatg atccgatccg gcgagctcga cacgaagttc
     4561 caccagatgt accgaggacc gtcgagcggc tcgccgccga gcagtaatag ccgcgccgga
     4621 ccgtcggctt cgagcaccgg atctgcgttc gccgcgaaca gcgccatctt gtgcggctcg
     4681 atgcgctggc catcggcgag cagtgcgccg tcgacgacgt agaccgcgcg ctcgcgatac
     4741 cccgccggca acgcgacccg gccgcctggc tcgagcgcga cgtcgacgta gaacatcggc
     4801 caaaacgtct cgaccggtga cgccgttccg aacgcattgc cgatcagcac gcgcgccttc
     4861 atgccggtct cgtcgacaat cggcagcgtt ggcgcaggat agtgcttgaa cgacggcgcg
     4921 ctctcttctt tctcgcgcgg caagccgacc cacagctgga tgccgtggtg tggccgcacc
     4981 tccgcacccg gcggcgtgcg ctcgctgtgc acgatgccgt ggccagcgct catccagttg
     5041 atcgcgccgg gtgtgatcag ctgcttcgag ccgaggctgt cgcgatgcat gacctcgcct
     5101 tcgaacaggt acgtgaccgt gccgaggttg acgtgcggat gcggacgtac cgccagctcg
     5161 ccgacctcgg cgcggatcat gtgatcgaag aacacgaacg ggccgacgaa gcgccggccg
     5221 acggtcggca gcacgcggcc gacggcaaag ccgcccaggt cgcgcgtgcg tgccgtaatg
     5281 atgcgttcga tagaagtgct catcccactc tcgcagtgcg cgtgatctca cccatccgcc
     5341 cgctctgata gtcgtggacc gcttcaatga gctcgcgctc ggaggtcatg acaaatggac
     5401 cgtagcgcgc caccggctcg cgatgcggga ctcccgccag caatagcaag cgtccacgct
     5461 gtgcgccgcc gcgcagccgc actgcatcgc cgtccccgag gatcgccatc tgcccgtcgc
     5521 ggacctcgcg gccctcgatc tcgatcgcgc cctcgaacac gtacgcgagc acctgctggt
     5581 ccttgctgat gtcgatcgtg acgtcagcgc ccggctccag cgtccagtcc tggaacacga
     5641 tcggcgtgtg cgtctcgatg gccgcgttca cgccgagcgc tgcacccgcg atgatctttg
     5701 cgcgtgcgag cgcgtcggca gtcttcgcct ccgggatctt cgcggccggt agctcttgat
     5761 agcgcggcgt cgtcagcttg agcttgcgcg gcaggttgac ccagatctgg aagccgtgga
     5821 cgcggccacc cttctcgagc agctcgcgtg gcggcatctc ggaatggatg attccgccac
     5881 cggccgtcat ccattgcacg tcaccggcgt tgatcacgcc gcgatgacct gcagaatcct
     5941 cgtgctcgac cgcaccttcg aggatgtacg agaccgtctc gaacccgcga tgcggatgat
     6001 cgggagcccc gaccgcttcg ccgggtccgt acgtcaccgg acctagctcg tcgatgagga
     6061 gaaatggatc gaccagctcg agccccatcg tggggaccgg ccggcgcacg acaaatccgg
     6121 cgccctcgcg ctggcgatgc gccgtcacca attgtttcac gttgcggttc atgggcagaa
     6181 cttggttact gccggtaacc gggtcaagac aggcacaacc gtgtgcctac cgggcgggcg
     6241 agacgctctg tctcacgcgc cctggatcag gccaatgtaa tttccgaacg ggtccgagac
     6301 gatcgagacc tggaggtcga caccgacgga atgcggcaat tcgatcgagc gcgcgccgat
     6361 cgtggtcagg tgctgccagg ccgcgttgat gtcctcgacg cgccagtagc cgatcacgcc
     6421 gctgccgccc ggcggtcgct tcgacgtgtc gggatcgagc ccgagctcgt ggccgtcgac
     6481 gtcgaagccg acgtagtacg gctcgtcgaa atacggatcc ttgccgagcg cctggctgta
     6541 gaactccttc gcaagcggca ggtcgtgaac ctgatagatg cacgtgcgga ggctgcgcag
     6601 atacggcccg accggcgccg cggcggtctt cttggccggc ttcttcgcgg cagtcttcgc
     6661 agcggccttg gacttcgaca acgaacgccc tgcgggcttg gccttcgcgg gctttttcga
     6721 cgcggacttc ttcgctgcgg gctttgcttt cgcttttgcc ttcgccatga acgtcctcac
     6781 ttcgctgcgg caaacgcgcg gtcgagatcg gcctgcaaat cggcgagatc ctcgataccg
     6841 accgacagcc gaataaagcc gtcgacgatg ccgagcttct tgcgattctc gggcggcacg
     6901 ctcgcgtgcg tcatgatcgc cgggtgctcg atcagtgact cgacgccgcc gagcgactcg
     6961 gcgagcgtga acagcttgca cgtcgacaag aagcggcgcg cgtgatcgag gttgccctcg
     7021 agcacgaagc tgatcatgcc gccgaagcca cgctgctgct tcttcgcgag cgcgtgctgc
     7081 ggatgcgact cgaggccggg atagatcacg cgctcgacct gcggatgctt gtcgagccac
     7141 gcggcgagct gccgcgcgtt ctgctcgtga cgatccatgc gcacgtgcag cgtcttggtg
     7201 ccgcggagca ccatgaagct gtccatcggc gagatcaccg cgccgactgc gttctgcaag
     7261 aacgccacgc gatcgcgcag cgcttggtcc ttgaagatca gcgcgccgcc gacgacatcg
     7321 gagtgaccgt tcaggtactt cgtcatcgag tgcgcgacga catccgcgcc gagctcgagc
     7381 ggccgctgaa agtacggcgt catgaacgtg ttgtcgacgg cgagcatgag gcccttcgcc
     7441 ttgcacacca cggcgacggc cgcgatgtcg acgagcttga gcatcgggtt cgtcggcgtc
     7501 tcgatccaga cgagcttcgt gtttggcttg attgccgcct cgaccgccgc ggcgccgccg
     7561 agcggatcga cgtacgtgaa ttcgaagccg agccggcgga acaccttgtc gaacagccgg
     7621 aacgtgccgc cgtagagatc gtcgcacgcg atcacgtggg tgccggcgtc gaacgcgtcg
     7681 agcagcgttg cggttgccgc gagacctgac gcgaagcaca gtccgccaac gccaccttcg
     7741 agcgacgcga gattcgcttc gagcgcgtag cgcgtgcggt tatgcgtgcg cgagtactcg
     7801 tagcccttgt gctcgccggg cgcggtctgc gcgtaggtgg acgtgtaata gatcggcgtc
     7861 atcactgcgc cagtcaccgg atcgggttct tgtccggcgt ggatcgcgcg tgtgccgaag
     7921 ccttcgctct tcgtcattcg aattggaacc ctttcggatc cttctccatg atccgctcca
     7981 tcgccgcctc ttcgagaaga tcgatcattc gctgaccgcc gtacttcttc atcagcttgt
     8041 cgtcgtactc gtccgcgatg agcgcgacat cgcgcaacgt tgccatcagc tcttcgggat
     8101 cgagtgtcgt gccaccgagg atcgtgtggg tgaacagcac ggtctgctcg cccggctccc
     8161 aggcgaacgc gccgaagcgg aggtgggcat tgagctcgag cagctgcttc gcgagtggtc
     8221 cgtccacatt gacgcccttc acgagctgtg acgtgcaccg caccatcgcc ttgttgtcgc
     8281 cccacggcac cacgctgatc atcacgtagg ccgaaccctg cttgatgacg taaagagagt
     8341 catcgatctt ccggtaggcc gggctgtcct ggagtgtgcg ctcgatcaaa agcgcggtgg
     8401 caacttcagc ggggttcatg aagcgttgtt aacacgggac gccgtgcgat actcggagac
     8461 gtgcgtcggg tcgcgctcgc gttattgatc accgccagca cagctgcgcg cgcacagacg
     8521 ccgtatccgg tgccggtgcc gcccgaggag ctcccgccgt caccgccgcc agcgccaccg
     8581 ccggatccac ggccgacacc ggtgccgctc gggcccgaca acaagccgat cgaacagcag
     8641 ccgcagcaac cggcgccgca gcaaccgcaa cagccgcctc agcagcagcc aactcagcaa
     8701 caaccgcagc cgcagcagcc acagccgcag ccgccgcagc cgcagccgcc gcagccgcag
     8761 ccgccacagc aaccgcagcc gcaaccgacg ccgccgccgc ccgatctgaa gatcgagcag
     8821 aagctcgagc cgaacgagac cctaccgacg gccgcggacc tcactgcatc cctcacgatc
     8881 aagaagatcg tcgtcgagga caacaccaag accacgtccg atacggtcga ggtgctcgcg
     8941 ggcatcgaga ccggcgacag ctggaacgcc agcatggtcg acgtcgtcaa gacgcgcctg
     9001 gtgtcgagcg gcctgttcaa ggacgtcgag gtgtactgga cgcgcatccc ggaaggtgtg
     9061 gtggtccaca tcctcgcgaa ggacaagcac tcgtgggtga tcgcgccggc gttctacaac
     9121 cagccgacga acgtcggcgg cggcattggg tttggcgaga acaacctgtt cggccagaac
     9181 cagaagctac tgctctacgg tcagatcgcg accggtgact cgttcttcat cggtgcgtgg
     9241 gtcattccgt cgatcggcgg cacacgcttc tactcgcagc tcgatacgta tctgaagagc
     9301 ggccgcaaca tcgagtacac cgcgcccgac agctggctcg tcgagaagga cgagatccgc
     9361 ccgatccgcg agtcgcgcat tcactacctg aacttcggcg tgaagctcgg catcgagctg
     9421 tggcgcggcg tcaaggtcga cacccgcctg cgcgcggcct ccgtcagtta ccaggatgtc
     9481 cacctctccg acggcgcgac gatggccgac atcaagccgg gccccgatcc gatggtcgtg
     9541 cccgctccag gcatggaagg caccgacgtc tcgaacgagt ggtccttcac gatcgatcgc
     9601 cgcgcgaact ggtacggcgt gcaggagggc aggaagtaca cggtgtcgta cgagcacgcg
     9661 acgccatcgc tcggctcgga cttccgctac tggctcgcga gcttcagcgc atttcgcgcg
     9721 tacaaggtgc tcgaccgcca caacttgatc ctcaaaggag ccgtcaacta cggccacaat
     9781 ttgccgttcc agcaggagtt ccagaccggc ggcacgtcga tgcgtggctg gctgaacaac
     9841 gagttccgcg gcgacttccg aacgctcgcg aacctcgagt actcggtgcc gatgttcacg
     9901 atccacgggt tctcggtgcg gggcctcgcg ttttgggact cggcctacac gacgttcatc
     9961 aacaccacgg acaacgagac ggacggcgtc cgcaactatc tgccgaattc agaggtgcgc
    10021 ggactcgacg gattcaagaa ttcagtgggc gtcggtacgc gtttctacct gcgacagatc
    10081 gtgctgccac tgctgggcct cgattttggg tatggtctcg aagcccgtga cgtccaaatc
    10141 tatctggcga tcggccttac tgattagcgc agcggttggg tgcacgagcg gcccgcgcgc
    10201 gactgcgccc gccgcgactg cagacgttgc ggatgtcgtg ctgctgcacg gcgacgtgtt
    10261 caccccgacc ggccacaagt ctgcggttgc ggttcgcggc ggagacatcg tcgcgtacga
    10321 cgacgcggag aagtggatcg gcggcaagac gcgcgtcctc gacctcgccg gcaagagcgt
    10381 gatcgtcggg ctcaccgacg cgcactgcca tctctacggg ctcggcgtcg acatggagaa
    10441 cgtgtcggtg cgcgcgctcg gcagcgaggc agaggtggtc aaggtgatct ccgacgccgc
    10501 gaagacgcgg cctgcgagcg agtggttgat cggccgcggc tgggatcaga atcgctgggc
    10561 cggtcagcaa tttccgacca aggcgacgct cgatgctgca gtcagcgatc gcccggtgtt
    10621 gctccgtcgc atcgatggtc acgcaatctg ggtgaacagc cgtgcgctcg ccgaggcagc
    10681 catcacgaag gcgacgaagg atccggccgg cggcaagatc gtgcgcgacg cgaacggcga
    10741 accgaccggc gtgctcatcg acaacgcgac cgatctcgtc gatgcgaaag tgccggctgc
    10801 aaccgccgac gtgcgcgagc gccgcattct cgccgccgcg aaggtggcca ccgagctcgg
    10861 ctttaccgcg gtccacgaga tgggcatcga ggacgcgacc gccgacgtct atcgcaagct
    10921 cgccgcggca aaccagctgc cgctccgcgt ctacgcatat ctcgccggcg acgccgcgca
    10981 tgccgacgag ctgcgcaagc gtccgcccgc gccagcgacc ggccggttcg tgatgcgcgg
    11041 cgtgaagtac tttgtcgacg gtgcgctcgg ctcgcgcggt gcgcggctct acgaggagta
    11101 cgacgatgac aaggccaacc tcggtctgtg ggtcaccgag ccgaagcagc tcgccgctgc
    11161 cgtcgacgcg tcggtcgcgg gtggctggca gacggcgatc cacgcgatcg gcgacgccgg
    11221 cattggcgcc gtgctcgatg cgtatctcgc cgcgcaggcg aagcatcccg gcgaccatcg
    11281 gctgcgcgtc gagcacacgc aggtgatcgc gatgaaggac gtgccgcgca tggtgcaagc
    11341 gcacgccgtc gcatcgatgc aaccgacgca cgcgacgagc gacatgccgt gggcagaagc
    11401 gcgcgtcggt aagcagcgca tcaagggcgc gtacgcgtgg cgcacgatgc tgaacgagaa
    11461 gattccgctc gcgttcggat ccgactttcc cgtcgaggaa gtcaatccgt tgctcggcct
    11521 cgactcggcg gtcaatcgcg gcaactggta cccggagcaa aagctgacgc tcgacgaagc
    11581 agtcgccgcg ttcacgaccg gcgctgccta cgcggttcat gaagaagcgc aagccgggcg
    11641 ccgcgccgac ctcaccgtct tcgacgcgaa gctgacgccg acggatctgc cgaagcgcaa
    11701 cgtgctgatg acgatggtcg gtggtgagat cgtgttcgaa cggcaaggcg cactcgcgga
    11761 ggccacgggg cgctgatgcg gttctgtccg ttctgcagcg ccgagaacgc cgacgagtcg
    11821 gcggtgtgct cggcgtgcgg ccggcgattg ccgccgctgc cgcctcggcg tgcgggcagc
    11881 aagggaccac cgacgggcgt gcagttgccg ccgcgtgcga gctcgccgcc gccgccgcgc
    11941 aagccgacga cgctgccgcc gccgattccg tccgctggtg tgccgaccga gacggcgttc
    12001 cgcgacagcg cgcccgcatt gccgccgcca ccaccgtcgt tcgaggatca agcgaccgag
    12061 gtcgcggccg agctgcgcaa cgcgatcgat ccggggcccg cggcgcagcg tcgtgaagcg
    12121 ccgccgatcg aggcgcccac cgcgggcgca ccggcgcgcc gcaagagcga ttcgaaggcg
    12181 ccaccgctcc cgccgaccat ggcgcgttct ggtggttccg gtgcgatcgg tacgccaacg
    12241 ccgacgccgg tgagaggtcc gggctccgga tcgctgccag cgtcggtcgc cgagcgcgcc
    12301 ggctcacacc acgacaccac gtacgaacgc tcgaagtcgc cggcatctcc gctcgagcgt
    12361 tcgaagtcgc cgtcgcaacc gccgcccttc gatcgctcga agtcgccatc gcagccgccg
    12421 ccgttcgatc gcgcgaagac gccgtcgcga ccgccgctcc cgcgtccggg atcgagtgga
    12481 ttcgggggcg gagagaccat ggtctcgcct ccgccgtcgt tcgagaccga gcccgccgca
    12541 tcggcgtatc cgctgcaagt cgcagcgaat cggctgcgtg catccgagat cgagccaccg
    12601 ccgactcgca tccaacgctc cgatgcgacg gaccggccgt tcacgccgca gaaggtcaac
    12661 gcggtgcccg agattcccga gcccggcctc gtcaacgcgg cgcggtatgc gtatcagttc
    12721 gcgctcgcgc gctggcaacg gcgcggcgcg atcaagaccc tcgggatcga aatcaagcaa
    12781 gacaccgagg cactcgacca ggtcctcggt gcgctcggtc gtgcggcacg cggtgcccgc
    12841 gtcgaagggc gcgtgttctc ggccgagaat gccgcgatct cggcggcgga ggagcgcgtc
    12901 ggatcgctgc aaaaagaatc cggcgacgtc gagggccgca aggccgagga gaactccaag
    12961 ttcgtcgacg tcgaacgcga acgaaacacg aagctcgtcg aggccgagcg tcaggtcgag
    13021 gaagcgcagc gcgagctcca gcacctcgaa gcccagcgcc ggagcctgcg cgacaagcgt
    13081 aaggagctcg accgccgcca gaaggcgtac ctcaagaacg ccgacgatca tgatcgtcag
    13141 gcgggagcgg caccgctcgg cgaccagcgc caggagttac gccgcgcggc cgagggccat
    13201 cgcaaggagg ccgcggcact cgagcccgac aaacaggaca ttgatcgaag gatcactgcc
    13261 ctcgagcggc cgatcagcga ggccaccgcg aagctcgacg ccgccaaggc cgagctggac
    13321 gcctccaagc gcagcctgaa cgatgtccgc gaaggtcaca cccaccgact ggcggagctc
    13381 gatgccgagc aaaagcgaaa ggcccgcgaa gtcgccctcg ccgaagccga aatccagcgc
    13441 cggctcgtca cgctcggcac gctcgtgaat ttgaaccgca tcgaggaccc tcagttcgcc
    13501 gagctgtacc agcgtatcga tcggctccgc ggcgctatca cggcccgcac gaccgagatc
    13561 gagaaactga ccgctgagcg cgaagcgtac gaccggggca cactcgtgcg tggagtcgcg
    13621 acgatcgggg gtgcatttgt tgcactcatt gccctgatcg tcatcctgcg cgcggtcctg
    13681 tagtacgctt aattctcacc cgtgccgaag gttctcgttt cgaacaacag cgagttgttg
    13741 cggcacttca ccgcagcgcc gttccaacgg ctcggcttgc agttgctcgt ggcagagacc
    13801 gcgaccgaag cgacggcgat gttcatgaaa gaagagccgc cgctcgtcgt gctcgatgcc
    13861 gagacggaag gcttttcgat cgcgaagaag tgcaaggctc acaatccggg cacgcgcgtg
    13921 attctggttg cgggcaagcg tctcaacggc gatcagatgc gcgaggtctc cgcgagcggt
    13981 tgcgacgagc tgttgatcgc gccgatgacg gcggacgagc tccacgacgt catcgcgatc
    14041 caactcggcg agccgcgccc cggcaccgaa gcgttcgcgg tcaccgttca gctcggcggc
    14101 aagaaggtcg acgcgaccgt ctcgaacctc tccatcgatg gcgtgcgcgt cgtcgttctc
    14161 gagccagtga gcgaaggaca ggccgtcgaa gtcacgatca cgcccgatgg cgagtcaccg
    14221 atcgcgatca agggcaccgc cgtgtgggcg caaccgcgcg acagcaagac cgtcgttggc
    14281 gtcgccttca acgagctcga cgccaaagcg aagacgatgc tcgcgaagct cacgcagtgg
    14341 caggtcgtca aagacggcga gcgcacgcgc gtcgtgttgc gcggcgactt caccgaggcg
    14401 acgcgcttcg acgagcttct cccggcgatg gtcggccgca tcgtgttcga catggcgcag
    14461 gtcacgtaca tgaactcgct cggcgttcgc gcgtggtgcg aatttctgcg cgaggcccgc
    14521 attcagggtt acgagttcca cgcatgctcg atcccgttcg tgctgcaggc ctcgatggtg
    14581 cgtgacgtga tcggtcgcgg cacggtgacc tcgttcttcg cgccgttcca ctgcatcagc
    14641 tgcgatcacc aggaagagcg cttgctgcag tcggcggcga tcctcgcctc caacctcgaa
    14701 ccgcccgtgt tcaagtgccc gaactgcggc ggcgcgctcg agttcgacga cttgcccgag
    14761 cgctacttcg cgttcctcgc agacaacgcg gccgagtagc gcgcttctat atgtgcccgt
    14821 cggcgcgttc gcgccgggcg gtaggaacgc ggtacgctag gcgtcatgcg caagctgttc
    14881 gtgcggtcgc tggtgatcgc attcgtggca tttgcgctgc tgttcggtgg cgagtttctg
    14941 ctcgagtggc ggcgtgctgg gttccccgtc tggggtgcgc agagctgggt tggcttcaac
    15001 aacgccaagc tcgtcgacat gctcagcccg atggcgcgcg cctacaacaa cgtgctcgcg
    15061 atgctgatcg cgaccatcgg tctcgcgatc ccgctcacgg cgaacatgca cacgccgaag
    15121 ctgatcgaga tgttcttgaa ggaccgcgtc aaccgctggg tcctgtcgtt catcgcgctc
    15181 ggcgccgcgc acgtgctgtg ggtcgagtac atgatcggtc cgcagttcgc gccgacgtgg
    15241 gcgatcaccg ttgcgatctg gctcgcgatc gccggttggg tcgtcctgtt gccttacttc
    15301 ttctacgtcg ttcggttcgt cgatccgtcg cgcttgctcg tccgtctgcg tgacggcacg
    15361 acgttcgtcg tggggaaggt cgccgatcgc gagttcgacc cctacgaggc acagctggat
    15421 gtctcgacgc gcataaacca gctcggcacc atcatcatca agtcgctcga tcggaacgat
    15481 cgcgacgttg cggccgaagg cacgtgggcg atcaagcgtc tgctcgacga ctacgacaag
    15541 cacaagtcgc gcatgccgaa ggagtggttc aaggtcgacc gcgccgactt cgtcggtctc
    15601 tccgacgagg ctctcgagat gttgagcgag aaccgcacgt ggttcgagat gaagtgcttg
    15661 caacagatcg agcacggctt cttgcgcgcg ctgcacggcg cgaacgatac ggtgtcgacg
    15721 ttctccgacg cgacgcgcgt gatcgcctgc cgcgccgatg cgcatcatga cgagcaagcg
    15781 ctgcgcctgt gcatccgctt cttcaacaac tacctgcgcg aagcgatcaa ggctcgcaac
    15841 ctacgcgcgg tgtacgacgt gtttcatcag taccgccgcc tcggtcgcga tcttgtcgat
    15901 cgccccgagc tgctacgcga gatcggcaac cacttcgcgt actacgcggt gatggcgcgc
    15961 acgtacggca tgttgttcgc gccgcagctc gcgctgttcg atctcggcta cgtcacgcgt
    16021 cgcgcctacg aacgcgcgtc gccggcggcg cccgacttgc tcaagaccgt gctcgcgctg
    16081 ccgcatcgca ccggcaacga tctgcacacg atggcagtga aggcgaagtt gatcctcggt
    16141 gggttcttcg tcgaaaaccg gctcgaggcc gaagccgatc tcgtgcgcaa gaacttgaac
    16201 gacgtcgact cggtcagcgt cgagcgcgcg gaagcggagc tactcgccgc cgagcgctcg
    16261 ttcttcgagg tcaccgatcg ccagctgaac ctcgagtggg taccgccgga gcgccgcgaa
    16321 ccactcaagc agttctgcaa ttcgctccac aacggcgcct aacgcactct acgcagctcg
    16381 cgtggcacga cgcatgtagc cgccctgggc catgcgcgtt gtcgaagttg tcatcgcagc
    16441 ggtgctggcg agcgcttgtg cgccgcatac gcagtacgcc gcgagcgcct tgagcgattc
    16501 caactcggag cacatgggca tctgcgatgt agtcgagcaa tgctccgtag gtggacgagg
    16561 ctcgaacctg gggctcgcca ttgccgtcgg tagtgcggtt gccggcgtac ttaccctcgc
    16621 gctcgtgcgc cacatcgccc tggattttcg ttcgccgcag cggagtgtga cggcggcgcc
    16681 gaagcgtaac tagagcttca caggcggctt cgccgtaggt tcgttcgtcg tcggtttctt
    16741 ctcgaccttg agcgagcccg tttgcgacgc ccacatgaag tagccgaccg tagcgagcgc
    16801 gatgatcgcg acgatcagga tcgcgttgaa cgggacgcga tcgccaaacg tcttgcgacc
    16861 gaagaagcga cccgctgaac gcttgtggat cgtatcggtg acatcgttcg tgaacgatgc
    16921 cggcgcgggt actggccgcg ccttctgcat ccccgacatg aacttctcgt tctcgatcat
    16981 gtcgtcgtgc gtctgcttcc aaactggatc ggtctcgatc ttcttcgcga cctcggcacg
    17041 ctccgccgca gtgagcttgc catcgacgta gtcggaaacc ttcgccacga tttcgtcgtc
    17101 gatatcttcg tctgcttcac tcatgttggt cacccaggtg gcgttgcagc ttcttgcgca
    17161 gcacgagccg cgcgcggtgg aggcgtgact tcaccgtacc gtcgggtagc tcggtgatcg
    17221 cgcagatttc ctcgatcgac atgtcctcga cgtcgcgtag gacaacgacg atgcgctgat
    17281 cttcgtcgag gcttgcgatc gcttctcgca tcagcttctc ggcttgtgcg ctctcgagcg
    17341 ccttgtgcgg cgacgatggc agcggcgcac cgatcgagcc gttgatgccg gggccgtgcg
    17401 tgctctcgtc gatctcttcc tgatcacgat tgtggcggcg cgcgaggtac ttgatccggt
    17461 tcttgcagtg gttgacggcg atccgataga gccacgtcga gaacttcgct tcctcgcgat
    17521 actgctcgat cgacttgaac accgagatga agacttcctg ggccacgtcc tccgcttcgg
    17581 cccggttgcc gagcatgcgg aacgtgatgt tgaagacctt gtcccggtgc tgttccacga
    17641 ggtgccggaa cgcgcgctcg tcgcgatcca gtagccgtcg gagcagcgcc ggatcgaggt
    17701 cagacatggg ccgcagtgag cataaccgtg tgaatcaata gacaccctag gcctgactct
    17761 ggtttcgctc ccggtacccg accggttggc cggcgcccat ttcggacctg gtgccatttc
    17821 gagcggtttt ggcgctcccg gagccgcgcg ctttctcaat agcttttcgt tcaaatgggc
    17881 tgtaacttgc ccattgccac ggttcgtcgg tctgcaggct gctcgcatgt tcgcgtttgg
    17941 ctaggtcccg gtgctcagtg ggcaagattc cgaaacccgc tttcgagcac aaacggcccc
    18001 tcgtggggcg ggtctagcta ctcatagggc cagttcgagc aagctgatga acactcgtct
    18061 gtacgttgga aacctttcgt tcaacacgac cgccgacggc gtccgcactg ccttccagca
    18121 gttcggcaac gtcagtgacg tccacctcgt gaccgatcgc gaaacgggcc gcagccgcgg
    18181 cttcgcgttc gtcacgatgg gcaccaccga agaagcggcc aaggcgatcg agggcatgga
    18241 tggcaagacc ctcgatggtc gcccgcttcg cgtcaacgaa gccgagcagc gccaggcgcg
    18301 cggcggcggc ggcggcggcg gtggcttccg cggcggcggc ggcggtggcc gtggcggcta
    18361 cggcggcggc cgtggcggcg atgactacgg cggcggtggc ggcggtggcg gcggcgactg
    18421 gggcggcggc ggtgatgacc gcggcggccg tggtggtcgc ggcggccgtg gccgcggcgg
    18481 cggcggcgat cgctggtaat cagcgtctgc tcagaggaaa gccggtgtgc gctgcgagcg
    18541 ctgccggctt ttccgttttc ggcggcgctt cggagcacgc cttgcagcag cgtagctacg
    18601 agatgtggct actgattcaa cgcgtgatcg ccggcatcaa gcgcgcgctc attcggcgac
    18661 atcgagcgcc ggagcggctt gccgcggacc gaagaaacgc aagctcgcga tgaacatcgt
    18721 cgcttcggcg ccgctcaccg cgtagcgcgc ggcaacgtcg accgcgagct tcatcgtcgc
    18781 atagccgaga cccgccgaca gataggtcgc gtccatcccg ttgtcgtgca gcacaccggc
    18841 gcggatcggg aagccagcct ggccgtcgcc cgggcgcagg aacagctccg cgccaccgcc
    18901 gtagcgcgcg gtgtgatcgt tatcctcgag cttccagcgc gcgtcgaagc tgagcgcgag
    18961 cagcggcatc ggacgcgcga ggacgccgcc gccgacagcg cgcgggaact cgggcgaatc
    19021 ggcgccccac aggttgtagc cagcagcgcc gatgttcacg agctcgctga tgcgtagcgt
    19081 cgcgccgaga tcccagttga agccgctcgc cttcatctcg ctcgcgaggt cggagtcgaa
    19141 gttgaagtac ttgaccgacg agccgatgaa cacgcgcggc gtgaccggat acgcgagcga
    19201 catgccgccg agatgcgtgc gacgcgacat cgccatgcca ccgagctcgg ggctcgagcc
    19261 ggagtactgg tagtaaaagc agccgggata cgcgccggtc gaatcgcacg ccgacacgcc
    19321 gaccagcgat gccgaatcgg acagccggta gccatagccg ccttcgaaca cgagctcgcg
    19381 attgagtggc agcccggccg gattgagacc gatggccgaa gcgcccgtgg cggcgccgcg
    19441 catcgcctcg ccgaccgcga tctcgcgtgg gccgaggttg tcgaccagtg catcgccttc
    19501 ggcgtacgcg ctgcccatca gcgagatgac catgatcagg gacgtccgga ccaaccgcat
    19561 gtcccgagtc ttggctcggt tcaggcaaat gcgcaagaaa cccgtggcat acgcagtgca
    19621 caaagccaca ccatgaagga caccatcgat gccctagaat tgctgaagga acagcacgac
    19681 gaagtcgaag acttgattac ccaaatcgag gactccgacg accccgatga caaggaggag
    19741 ctgttccaga agcttgcgga ccggatcgcc gctcactcga cgatcgaaga gaagctcttc
    19801 tatcccagcg tgatgagcga ggagacccag gaactgcttc tcgagtccac ggaggagcat
    19861 ctggccgtca agcgcgtcct cgccgacatg ctcgacatgg acgccgacga tgaacgcttc
    19921 gatgcgaagc tgacggtgct caaggagagc atccgccacc acgcgcgcga cgaggaagag
    19981 ggaatgctgt tcccgatcct gcgccggcgt ctgagcgccg agacgctcgc tggtctcggt
    20041 aacgagttgc tggcgttctt cgagaagttg ctcgagcgcg agccgcgcat gaacgtgccg
    20101 gccgagacga acgccgcggc gccgctcgaa ctgggtatgt agcgcacact aggcagcggt
    20161 tgcgcgccgc acgcgacgcg cttcgccgat gacatcatcg accacgctga ggtcgccgct
    20221 cgactcgaga tggatctcga gcacgtcggg cagtttcagc cgattggcct tcgtgcgcac
    20281 ggcctcttcg aggtcggcgt ggctgatgcc ggcgcggcgc atcgcagcga gctcgaggat
    20341 gccgccgcga tacagcacct tctgcctccc tttgaacaag cgctcgagcg cgggcaccgt
    20401 agccgtcacg atcgcgacga ggcgatgcag cacgacgaac acgatcgacg cagcgaccgt
    20461 cggaatggcg ggcgacacac cgacgacgac gcgtgagagc accgcgccga gtgtgatcac
    20521 gatgatcgtg tcgaacgatg aatgccgccc gaatgcgcgc atgcctgcga cgcgcacgag
    20581 caccagcatc aagaagaaca gaacgaacgc acgcgacgac atctgcagcg cgctgaggtc
    20641 gcggccttcg ccgaacaatt gctctacgct catacgctcc ggacgtgcat gcgatgttcc
    20701 ggtggtacgc gcgctgcatc ggctaagccc cgatgtcggc gagctcgagt ctctcgaccc
    20761 ggtatcgcag cgtgcgacag cgctcgctga gcgtgtgcgc gccgctgagc gccgaggacg
    20821 cgcaagtaca gtcgatgccg gacgtcagtc ccgcgaagtg gcacctcgcg cacacgacgt
    20881 ggttcttcga gaggttcgtg ctcgcgcgcg tcggcgattc gccgctcgat ccggcgttcg
    20941 agtatctgtt caacagctac tacgacagcg tcggcgcgca tgtcgatcgc gcgcggcgag
    21001 gccacctgac acgtcctgca ttgccagtcg ttcacgcgta tcgctacgcg atcgacaccc
    21061 gcatcgaaga ggtgctcgcc gacaaacccg acgtagaaac gctcgctcgc atcgagctcg
    21121 gtctgcatca cgagcagcag caccaagagc tgatgctgac cgatctcaag cacgtgctcg
    21181 gcatgcaacc gctgcggccc gcgtaccgcc ccgatctcgc gcgtcccgca aacgccaccg
    21241 tcgagatgcg gcattggatc gagatcgatg gtggcgtggt cgaggtcggc gcgaagggcg
    21301 ccggcttcgc ctacgactgc gagcggccgc ggcactgcgt gctgttgtcg tcgtaccggc
    21361 tcgcgagtcg gccgctgtgc aacgccgagg tcctggcgtt catcgccgat ggtggctatc
    21421 gggactcacg gttctggctc gccgatggct tcgcgctcgc gcgcagcgag aactggcggg
    21481 caccgctcta ctgggaacaa cgtgatgacg tatggctgca ctacacgctc gcaggtttgc
    21541 gcgaagtcga tccggctgag acggcgtgcc acctcagcta ctacgaagcc gacgcgatcg
    21601 cgcgctgggc cggtgcgcgc ctaccgaccg aagccgaatg ggaacacgca gccgcgcgcg
    21661 tcgagcttac cggcaacttt gccgacgacg atgcgctgca tccgagcaca gcgtctggcg
    21721 acggcctgcg ccagttgttc ggcgacgtct gggaatggac gcagagcgca ttcgcgccgt
    21781 acccgggctt tcgtccactc gacggcgcgc tcggcgaata caacgggaag ttcatggcga
    21841 accagctcgt gctgcgtggt ggttcgtgtc tgacgccgcg cgatcacatg cgcgcgagct
    21901 accgcaattt ctttgcgcca tcggcgcgct ggcaaatgac cggcgtgcga ttggcgctcg
    21961 atggagagca gtcatgaaca tcgccgcacg tgcaccgatc ccccaagacg atatcctcac
    22021 cggcctgctc tcgcggccga agcgactgcc gtgccgcttg ctctacgacc ggcgcggtgc
    22081 cgagctgttc gatcggatca cgtgcctcga cgagtattat ccgacgcgcg tcgagctgcg
    22141 cttgcttgac gagtgcttgc cgaagatcgc ggccaacgtt ggtgtcgccg cgcgcgtgat
    22201 cgagccgggc tcgggtgcgg gcatcaagac caagcgtctg ctccgcgcgc tcgatcaagc
    22261 ggcgagctac gtcggcatcg atgtttcgcg cgaatcactc gcgtacaccg cggaggtgct
    22321 ctcccgcgaa catcctcgcc tcgacatcca cacgatcgag ggcgacttca cgcggccgtt
    22381 cgcgctgcca acgccccgcc gtccggtggg gcgcacactc gtgttctttc ctggctcgac
    22441 gattggcaac ttcgagccgc acgaagcagt gatgttcctg tcgggactgc tccgcggcgt
    22501 aggcgcgaac agccggctgc tcgtcggtgc cgacggcacg cgcgaacgcg aggtcctcga
    22561 gcacgcgtac aacgactacg aaggcgtcac tgccgagttc aacaagaacg tgctcgcgca
    22621 tctcaaccgg acacgcggca cagcgttcga tctcgacgcg ttcgaccatc gcgcgatctg
    22681 gagcgagcgg ctctcgcgca tcgagatgca tctcatcgcg aagcgcccgc agcacgtcat
    22741 cctcgacggc accgcgatcg tgctcgcgcc cggcgagtcg atcatcaccg agtactcgta
    22801 caagcacacg ctgaccgcga tgcgcagcat cctgctcgcc gccggctggc gcgtgagtga
    22861 cgtctacacg ggcaaagagc aaccgatgcg gctgtggcta tgcgagcccg cccaggagta
    22921 gagttcggcg ccatggccaa gtcagattcg ccgctcgtcc aggccgccac gcagttcgac
    22981 gaggagctcg cgatctactc gcgcctcggc gagctgtttc tcaagacgcc gctcacatcg
    23041 ctcaagcagc tcgagcgcgc gaatcagatc ctcggtgaga tcgcggactg cgagcaacgc
    23101 ttgtccgacg cgggcaagcg cctgatcgaa gcgctgaccg gcgcacgcca gacgcaggaa
    23161 ggactctcgc agagtgtcgt agcgcacgcg ccgaccgtgc aagcgcgcaa cacgaagctg
    23221 cgcgagttga tggcggcgat ggctgcactc gcgtccgacg tgcagggcgt gaatgcgctc
    23281 gtgctgcaaa agaacggcga ccagcagcaa gacgcggcga agccggatcc aggcgacgta
    23341 tccggaaagg tgctcgagct gtcggagcgc gccgaggcgc tcgcaacttc gtgccgcgag
    23401 gcagagtttg ccgagctcgc cgagcaagcg cactcgctgc atcagcgatt gaaggcgatc
    23461 gggacgaagc tgcaaaaggc cgccggcaac tagcgcgttc ccccgaagac gtgggcccga
    23521 ttgcttggag ccaaacgatt gtggtcgaca tgcatcgcca tccaacgcga tgatctatct
    23581 ctgcttgtct tccgctatcg cggcgtgtcg tgggggccca tgaacagcct gaaactatcc
    23641 gagctcagca tcgacgtcgt cgagaccccc gagtcgacgt ttcaatgcac ctggcgcggc
    23701 aagagctacg agcgcaacgc ggccgacacg ttatcggcgt ggctcgacgc actgctcgca
    23761 accgctgtcg ccaaaggcgg tgcgatcgag atgcacttcg aggacatcga gcacttcaac
    23821 gcgtcgacga tcacggtact cgttcgccac atccaggcgt gccgggcgca gaaggtcaag
    23881 ctgacgatgg tctacaacgg cgcgctcaat tggcagtcac tgtcgttcga cgcgcttcgc
    23941 atcttcgtga tgcgtgacga tctcttcacg ttgcggccag cgtgacggca ttcacagcgc
    24001 atcggtcggg cccactggac cgcaatcgat cgactggtgc atcgaggttg atgtgctgcc
    24061 ggcgaacccg ccggcctcga accgtggctg ggcatgcgtc gctggcgcga tcgcaccgac
    24121 ggccgtggtg acgaacacgt acgttgtacg tctcagcgtc tgcatcgtcc tgcctcgcga
    24181 ccatagttcg gatcgccggc aattactgtg gcgaggatcg ctctcggttg cagctgcagc
    24241 tgcgatctct gccgaaatca aacgcgggcg agagctctcg gcatgactcg cgccgaaaac
    24301 gaaacagccc gccgagcgcg cgggctgtga gtagtcagat ggctcgacgc gcggctactt
    24361 cttgccgccc ttgtcgttgc ccttcttctt gtcgtcttgc tgcttcttgt cgccttcctg
    24421 cttcttctgc tcgtcctgct tcttcttctc gtcggcttcg gccttcttgc ggttttcttc
    24481 gtcctgcttc ttgcgcgcct cgtcctgctt gcgctgctcg tcttgctggc gcttcgcgtc
    24541 ttgctcggcc ttcttgcggt cttgctcctc ttgacgcttg cgcgcttcgt cctgcttgcg
    24601 ctgctcctct tcctgcttgc gcttctcctg ctcggccttc ttcgcgtcct gctcctgctg
    24661 gcggcgctgc tcctcttcct gcttgcgctt ctcttgctcg aggcgcttcg cttcttgatc
    24721 ggcgcgcttc ttctcttctt gctgacggcg ctgatcctcg gccgccttct tgcgtgcgtc
    24781 ctcttccgcc ttgcgcttcg cttcctcgtc gcgcttgcgt tgctcttgtt cggccttgcg
    24841 cttctgctct tcgaggcgct tcgcgtcttc ctggcggcgc ttctcgagct cggcggctcg
    24901 cttcgcttcc tcttccttct gcttgcgctg ctgctcctcg aaccgcttct gctgttcggc
    24961 gcgctggcgc tcctgcgctt cttttgcttt gcgctgttcc tcgtcccggc ggcgctgctc
    25021 gtcctgcatg cgcttcgcct cggcctcggc cgcgcgacga cgctcgtcgt ctagcttctt
    25081 gcgctgctct tcgatcttgc ggcgctcgtc ctcgaggcgc ttgcgctcgg cgtcgttacg
    25141 cgcgcgcgct tgctgctctt cgaacttctt gcgctgctct tccaggcgct ggcgctcctt
    25201 ctcctggcgc tcgcgctcct tggcctgccg cttctgctcg tcggcgaggc gttgctcttc
    25261 ttgtttttgg cggcgctgct gctcttcgag ttgcttgcgc acctgctctt cgcgcttgcg
    25321 gcgctcgtcg aactccttct tgcgctcgcg cgcgcggcgg tcggcttcgg ccttgcgttg
    25381 cgcttcgaag cgaccgagct ccgacggatc ggagcggtct ttgcggaagt agacgttgta
    25441 gcggcggaac cagtcttcgc tcgggcccgc gacccactgg aacttcttga acttgttgta
    25501 gcgaaggatc ggctggctcg tggacaggta gaccttgtag ttgttggtca cgaagcggcg
    25561 attgatgtcg accgcgaaca gatctggcgc gtgcagccag cgccacgaat cctcgggcac
    25621 gtaggcatcg ttgtcgtaac cggccggcga ccagccgatc cagccgttgc ccgcgcgcca
    25681 gttcacccaa gacggacccc acgtgagatc g
//
LOCUS       DASUFX010000721        11414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_393379,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000721 DASUFX010000000
VERSION     DASUFX010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393379"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1000
                     /locus_tag="VFV99_29195"
     CDS             143..1000
                     /locus_tag="VFV99_29195"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5063502.1"
                     /translation="MQSRAFGSVQVPVLGIGTWKMEYDERAAAIAAIRRAVELGMTHV
                     DTAELYGSGGVETLVAKALDGLRDRVFLVSKVLPHNASYAGTLEACEKSLRRLRTDVL
                     DCYLLHWRGDEPLAETFRAFEKLREQGKIRAWGVSNFDHDDLAEALSIAGEGRIACNQ
                     VAYHLGNRTIEHHVIPFCEQHRIAVVAYSPFGSGLFHSTPKLDAIAQRVGATPHQLVL
                     AFLTRRPPRVTDPSGPARPERSVLAIPKSANVTHVEELAHTVSLDDATIREIDALYPL
                     RPWRGLHTL"
     gene            997..2175
                     /locus_tag="VFV99_29200"
     CDS             997..2175
                     /locus_tag="VFV99_29200"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5063503.1"
                     /translation="MSDREFDLVLFGATGFTGKLVADYISKQKPKRWAIAGRNQAKLD
                     ALGFDAPSVIVDAMDPNACAAVAKRTRVVCTTVGPYSKYGSALVAACANAGTHYCDLT
                     GEVHWMREMIDANHERARKSGARIVHTCGFDSIPSDVGTWATQQEFIKRFGRPASKVT
                     AFYGEQSGTLSGGTAASGFAFAEAMSDPAVRRVLSNPFALDPDPDGPHPKTDDRMVGW
                     DPHLKMFFVPFFMAATNSPVVRRGHALAGYPWGKDFVYREVMSTPGNPRGAVMAATII
                     AGLAGLSAMMKRPRLRAMLQKRAPQPGEGPDAERRARGHWKVRFFAEDGNDHLLYIAA
                     DPAGDPGYASTAKMLGESALCLAYDRLATAGGCLTPSVAMNGQLLERLRSAGIAFHPG
                     "
     gene            2208..3668
                     /locus_tag="VFV99_29205"
     CDS             2208..3668
                     /locus_tag="VFV99_29205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5063504.1"
                     /translation="MAYRILAWFLRVVTRVFFRQIEVVGLEHVPKTGPVLFAGNHPNS
                     LIDPILIITTCGRKVHFAAKDALFKGRLMRAVLRGLGAVPIKRRDDHDGKPRAKTDPS
                     APPVDNDAAFEAMFAVLGEGGTIGIFPEGLSHDEAQLSKLKTGAARLALGGAQRTPLP
                     IQIVPCGLTFIHPKRFRSRVLVQFGPPLTVDATRPNHADEVKAVTGEIETALRRLTVN
                     APDWDTVRALDVVRRLYQPQEISIEDRVELARRFNAYYGAVAADPRVVALMSRVRAYQ
                     TKLDELGITDRELARDLSKLEVGARVLRHFVLVAFWLPLTVPGAPLHLPPLAFARVAS
                     AKLTPRKDVIATTKLLIGLLLVLAAYAATVAVLWWKVGYWLALWAIFLLPLSGYATLR
                     VLDRLRLVRRGLGVLFRRLRFRREVHALRRERDGLVDEVIATVNAVKPKDLDLKFPLD
                     HPDRLEESWRSRREADLDAEFDKDAAEAAAEDAEPR"
     gene            3665..4303
                     /locus_tag="VFV99_29210"
     CDS             3665..4303
                     /locus_tag="VFV99_29210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5063505.1"
                     /translation="MKPLVVLLHGLARGHGSMAKLGGYLRRHGFETWSHTYPSRQHSI
                     AYLASALADQLVDVAGERPLHAVTHSMGGIVVRHLRDPRLQWARIVMLAPPNQGSRLA
                     AGLVRNPLFKWFYGPAATELADASAWPDPPAPFAVIAGTRNLALSNVTSWTVGRRFPA
                     GVRNDGTVAVDETRLPGMAAFAEVDATHTWIMNSPEAWRLTSAYLRNGRFGP"
     gene            4347..4973
                     /locus_tag="VFV99_29215"
     CDS             4347..4973
                     /locus_tag="VFV99_29215"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX5063506.1"
                     /translation="MFGRFLPRETSFFDFFEQHAGLTIEGTKEFLSMVTTGANVATKC
                     RRISDIEHETDTITHRCVEALHKTFITPIDRDSIHRLITRMDDIMDFVEAAAERIELY
                     EITTMTADVRDLADVLHRSCLQVEQAIHGLRTLKTPQQTLKLCIDINRLENEADAILR
                     RSVARLFKDEKDPITVIKWKEIYESLENASDRCEDVANIIEGVILEHS"
     gene            4977..5987
                     /locus_tag="VFV99_29220"
     CDS             4977..5987
                     /locus_tag="VFV99_29220"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5063507.1"
                     /translation="MDATTIVYLTVAVALVFDIINGFHDAANSIATVVSTRVLTPRLA
                     VLWATLFNFVALFVFHQGVANTIAKIITIEASDHAFVWVVMSGLVGAIVWNLLTWWWG
                     LPSSSSHALIGGLSGAGVAYGGTDLLNTDKLYPTLQFIILAPLIGLVLGGLIMFIVYW
                     LFRGMRPRKVDRVFRVGQLFSAAAYSVGHGGNDAQKTIGVIWAVMIAGGLLPKEGAHV
                     PYWVIVASYGAMAVGTALGGQRIIKTMGMGITSLKPVGGFCANLAGAITLFGATHLKI
                     PVSTTHTITGAIVGVGSVTKVRGIRWGLATRIVWAWIFTIPLAGLVGAAMLLAAQALH
                     AW"
     gene            6029..6634
                     /locus_tag="VFV99_29225"
     CDS             6029..6634
                     /locus_tag="VFV99_29225"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003300.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX5063508.1"
                     /translation="MAKLYFRYGTMDSAKSMNLLAVAHNYRKQGKKVLLVKPRLDTRF
                     GATKISSRCGLEADADLMVEEDTVLDPRDFADLDCVLVDEAQFLAPSIIEDLRRITVD
                     PGVPVICYGLRTDFQTKLFPGAQRLMELADRIEEVKVTCQYCAKKAICNLRFVNGTPT
                     VVGPQIQLGADEHYAPMCWAHYDEATKHITAEQSGAVLRPS"
     gene            complement(6656..8539)
                     /locus_tag="VFV99_29230"
     CDS             complement(6656..8539)
                     /locus_tag="VFV99_29230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063509.1"
                     /translation="MEIVGLEPRRSDLARGILIARSTRPLDAAAQKALWAALSAAGVT
                     EKADEDADSEPTRHWHAAPWLPPTSHEVDPQRVALEATAEPFSIDAVWLVKVETGVDG
                     IAWLELASVEGTSDEDEPEASEPAEVVDAVFDDAEEVLYAAPDAPPPEATVDDDDSDE
                     DDDEGLGELEVVTLESHWRNGPPPFENSVPFPIERYPEMIDDYDWESFGIAFKLAGTA
                     SLGEESVINAFFALWLSAYQDERSEEFEPFQRADVIHDRKHRSALMWVERFAVPATAS
                     DQVHFLLWIVARLHDILPIAWARFEPVDDAVKAQAQGGEGIDPFVLAGNPFADRFQRQ
                     GEEAALQWAVGQTSWSRREIAGMLVEVALDHDPDNPATAVIAERLLRRAQSFDAGSDA
                     AGYLTIVLVRQGRLADAISLATQARSRDVRLLVIGEIAEHAPNDLAPALDLLDEATLD
                     ETEPEELADLTASVARHAPNQLAGLLAKLPDDVSLVPHLYNASFSVDRPLALSILRRV
                     LTLPEPPRGAGEARTALVMAWNNACIHAHALGDYKLAVELADGGQRYAPENPYIYHSA
                     ACAYAAIGQVDNALVQVQRAIEHEYEHTEKMETDADLSPLHSDPRFPALFNEWRTRRA
                     DLN"
     gene            complement(8572..9957)
                     /locus_tag="VFV99_29235"
     CDS             complement(8572..9957)
                     /locus_tag="VFV99_29235"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX5063510.1"
                     /translation="MHISVFDIFKIGIGPSSSHTVGPMRAARRFAERLEIDGVLAQVE
                     GVKIELFGSLGFTGKGHGSDKAIILGLEGEDPATVDVDAVARRVAVVEQSKRVKLLGK
                     HEVDLDPATSLVFHRREKLPLHANGMRFSALGPAGANAPLAERIYYSVGGGFVVDHAG
                     APADGSTPADQVQVPYPFNSGEELLRHAGEHGMAISSLMLENEKALLPETEIRRRVLE
                     ISIAMDQCVERGCKREGILPGGLKVKRRAAALFRKLKSDPRGDRDPLVIMDWVNLFAL
                     AVNEENAAGGRVVTAPTNGAAGIIPAVLTYYRRFIPGADDEGAVRFLLTAAAIGALYK
                     KNASISGAEVGCQGEVGVACSMAAGALAEVMGGTPAQVENAAEIGMEHNLGLTCDPIG
                     GLVQVPCIERNAMGSVKAINAARLALQGDGTHKVSLDKVIKTMWRTGADMSSKYKETA
                     RGGLAVNIIEC"
     gene            complement(9989..11215)
                     /gene="serA"
                     /locus_tag="VFV99_29240"
     CDS             complement(9989..11215)
                     /gene="serA"
                     /locus_tag="VFV99_29240"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5063511.1"
                     /translation="MAETSFPKSEIKILLLENIHPVAKEEFAAEGFQVETVKGALSEA
                     ELVDKIRDVHVLGIRSKTQVTPRALDEARRLLTVGAFCIGTNQINLGHANRRGTPVFN
                     APFSNTRSVAELIIAEIVMLSRHLGDRVREVHAGQWKKVAVGSHEVRGKTIGIIGYGH
                     IGSQVGVLAESFGMRVMFYDIAAKLPMGNNRSLPKLEDVLAESDFVTLHVPETPQTKM
                     MMGHEQIARMKKGACLLNASRGTVVDIDALAAALKSGHLGGAAVDVYPEEPETNSDGF
                     ASPLRGLGNVILTPHIGGSTEEAQEAIGKEVSAALIKFINTGTTAGAVNFPPVELPIT
                     RGAHRILNIHRNVPGVLRDINKIVSDLNANIRAQLLATDPDIGYLIMDLDQDVSQDVR
                     KAVAGLETSIKTRILF"
BASE COUNT         1925 a         3791 c         3661 g         2037 t
ORIGIN      
        1 cgtcgtgcag caatgccgcg accgccgcgc gctttcgccc gtccttgtcc ggagcagtcc
       61 tcgcccgctc cgccagtttg aggcgagtta ccaggcgctc gatgaacggc gacacactca
      121 agcatagcgt aggatgcgcg cgatgcagtc gcgtgcgttc ggatccgtgc aggtcccggt
      181 gctcggcatc ggcacctgga agatggagta cgacgagcgc gcggctgcga tcgcggcgat
      241 ccggcgcgcg gtcgagctgg gcatgaccca cgtcgacacc gccgagctct acgggagtgg
      301 aggcgtcgag acgctcgtcg ccaaggcgct cgatggtctg cgcgaccgcg tgttcctcgt
      361 gtccaaggtg ctgccgcaca acgcgagcta tgccggcacg ctcgaggcct gcgagaagag
      421 cttgcgcaga ctacgtacgg atgttctcga ctgctacctg ttgcactggc gcggcgacga
      481 gccgctcgcc gagacgttcc gtgcattcga gaaactgcgc gagcagggca agatccgcgc
      541 gtggggcgtg tcgaacttcg atcacgacga tctcgccgag gcgctgtcga tcgccggcga
      601 aggtcggatc gcgtgtaacc aggtcgcgta tcacctgggt aaccgcacga tcgagcacca
      661 cgtgattccg ttctgcgagc agcatcgcat cgccgtcgtc gcgtactcgc cgttcggtag
      721 cgggttgttt cacagcacgc cgaagctcga cgcgatcgct cagcgtgtcg gcgcgacgcc
      781 gcaccagctc gtgctcgcgt tcctcacgcg ccggccaccc cgtgtcacag atccgagcgg
      841 tccggcccgg ccggagcgct cggtgctcgc gattcccaag tcagcgaacg tcacccacgt
      901 cgaggagctc gcgcacaccg tttccctcga cgacgcgacg atccgtgaga tcgatgcgct
      961 gtacccgctc cgaccttggc gaggacttca cacgttatga gtgatcgcga attcgatctc
     1021 gtgttgttcg gagcgactgg cttcaccggc aagctcgtcg cggattacat ctcaaagcag
     1081 aagcccaagc gctgggcgat cgccgggcgc aatcaggcca agctcgacgc gctcggcttc
     1141 gacgcaccga gtgtgatcgt cgacgcgatg gatcccaacg catgcgccgc cgtcgcgaag
     1201 cgcacgcggg tcgtgtgcac gaccgtcggc ccgtactcga agtacggcag cgcgctcgtt
     1261 gccgcctgcg ccaacgccgg tacgcactac tgcgatctca ccggcgaagt gcactggatg
     1321 cgcgagatga tcgatgcgaa tcacgagcgc gctcgaaaga gcggtgctcg catcgttcac
     1381 acgtgcggct tcgactcgat cccgagcgac gtcggcacgt gggcgacaca gcaggagttc
     1441 atcaagcgat tcggccggcc ggcgtcgaag gtcaccgcgt tttacggcga gcagagcgga
     1501 acgctgtcgg gcggcaccgc tgcaagcggc ttcgcgttcg ccgaggcgat gtccgatccg
     1561 gccgtgcgcc gcgtgctcag caatccgttc gcgctcgatc ccgatcccga cggaccgcac
     1621 ccgaagaccg acgaccgcat ggtcggctgg gatccgcacc tcaagatgtt cttcgtgccg
     1681 ttcttcatgg ccgcgacaaa ctcccccgtc gtgcgccgcg gccacgcgct cgccggctat
     1741 ccgtggggca aggacttcgt ctatcgcgaa gtgatgagca cgcccggcaa tcctcgcgga
     1801 gccgtgatgg cggcgacgat catcgccggg ctcgccggcc tgtccgcgat gatgaagcgg
     1861 ccgcgcctgc gcgcgatgct gcagaagcgc gccccgcaac cgggcgaggg ccccgacgcc
     1921 gagcgccgcg cgcgcggtca ctggaaggtg cggttcttcg cggaggacgg caacgatcac
     1981 ttgctctaca tcgccgccga tccagcgggc gatccaggct acgcgtcgac cgcgaagatg
     2041 ctcggcgagt cggcgctctg cctcgcgtac gatcgactcg caaccgcagg cggctgtctc
     2101 acgccgtcgg tcgcgatgaa cggacagctg ctcgagcggc tgcgcagcgc cggcatcgcg
     2161 ttccacccgg gctgatcttc acacgcacgc gctgatacga tagatgcgtg gcgtaccgca
     2221 tcctcgcctg gttcttgcgc gtcgtcacac gcgtgttctt ccgccagatc gaggtggtcg
     2281 gactcgagca cgtgccgaag accggaccgg tgctgttcgc cggcaatcat cccaactcgc
     2341 tgatcgatcc gatcctcatc atcacgacgt gcggccgcaa ggtgcacttc gccgcgaagg
     2401 acgcactgtt caagggccgc ttgatgcgcg cggtgctgcg cggcctcggc gcggttccga
     2461 tcaagcggcg cgacgatcac gacggcaaac cgcgcgcgaa gacggatccg agcgctccgc
     2521 ccgtcgacaa cgacgccgcg ttcgaggcga tgttcgccgt gctcggcgag ggcggcacga
     2581 tcggcatttt tcccgaaggc ctctcgcacg acgaagcgca gctctcgaag ctcaagaccg
     2641 gcgccgcgcg actcgcactc ggcggtgcgc aacgcacgcc actgccgatc cagatcgtgc
     2701 cgtgcgggct cacgttcatc catccgaaac gcttccgcag ccgcgtgctc gtgcagttcg
     2761 ggccgccgct caccgtcgac gcgacgcggc caaaccacgc agacgaggtc aaagcagtga
     2821 ccggcgagat cgagactgcg ctgcgccggc tcaccgtcaa cgcacccgac tgggacaccg
     2881 tgcgtgcact cgatgtcgtg cgccggctct atcagccgca ggaaatctcg atcgaagatc
     2941 gcgtcgagct cgcgcgccgc ttcaatgcgt attacggagc agtggccgcc gacccgcgcg
     3001 tcgtcgcgct gatgtcacgc gtgcgcgcgt atcaaacgaa gctcgacgag ctcggcatca
     3061 ccgatcgcga gctcgcgcgt gatctgtcga agctcgaggt cggcgcacgc gtgctgcgtc
     3121 acttcgtgct cgtcgcgttc tggttgccac tcaccgtgcc cggcgcgccg ctgcacctcc
     3181 caccactcgc attcgcacgc gtcgcgagcg cgaagctcac accgcgcaag gacgtgatcg
     3241 cgacgacgaa gctgctgatc ggcctcttgc tcgtgctcgc cgcgtatgcg gccactgtcg
     3301 cggtgttgtg gtggaaggtc ggctactggt tggcactctg ggcgatcttc ctgctcccgc
     3361 tctccggcta cgcgacactg cgcgtactcg atcgactgcg cctcgtgcgg cgcggcctcg
     3421 gcgtgctgtt ccgtcggttg cgctttcgcc gcgaggtgca tgcactgcgg cgcgaacgcg
     3481 atggtctcgt cgacgaagtg atcgcgacgg tcaacgctgt caagccgaag gacctcgatc
     3541 tcaagtttcc tctcgatcat ccagaccggc tcgaggagtc gtggcgatcg cgtcgcgaag
     3601 ccgacctcga tgccgagttc gacaaagacg ctgcggaagc cgcggccgaa gatgccgaac
     3661 cgcgatgaag ccactcgtcg ttctgttgca cggtctcgca cgcggccatg gctcgatggc
     3721 caagctcggt ggctatctgc gcaggcatgg cttcgagacc tggagccaca cgtatccgtc
     3781 gcgtcagcac tcgatcgcgt acctcgcaag cgcgctcgcc gatcaactcg tcgacgtcgc
     3841 cggcgaacgg ccgctccatg cagtcacgca ttcgatgggc ggcatcgtcg tgcgccattt
     3901 gcgcgacccg cggctgcagt gggctcgcat cgtgatgctc gcgccaccaa accagggcag
     3961 tcggctcgcc gccggcctcg tccgcaaccc gctgttcaag tggttttacg gaccggcagc
     4021 gaccgagctc gccgatgcgt ccgcgtggcc agacccaccc gcaccatttg cagtcattgc
     4081 cggcacgcgc aatctcgcgc tgtctaacgt gacgagctgg acggtgggcc gccgctttcc
     4141 cgccggcgtg cgaaatgacg gcacggtagc ggtcgacgaa acgcggctgc cagggatggc
     4201 tgcgttcgcg gaggtcgacg cgacgcacac gtggatcatg aattctcctg aggcatggcg
     4261 cctaaccagc gcttacctga ggaatggccg gttcggcccg tgacctaaag atcccgcaag
     4321 tgctatatcg cggcgcagga ggctgcatgt ttggtcgctt tctgccgcgc gagaccagct
     4381 tcttcgattt tttcgagcag cacgcaggcc tgacgatcga aggaaccaag gagttcctgt
     4441 cgatggtcac gacgggtgcg aacgtcgcga cgaaatgccg gcggatctcg gatatcgagc
     4501 acgagaccga cacgatcacg caccgctgcg tcgaggcgct gcacaagacg ttcatcacgc
     4561 cgatcgaccg cgattcgatc caccgcctga tcacgcggat ggacgacatc atggatttcg
     4621 tcgaggctgc cgccgagcgc atcgagctct acgagatcac gaccatgact gctgacgtcc
     4681 gcgatctcgc cgacgtactg catcgctcct gcctccaggt cgagcaggcg atccatggtc
     4741 tgcgcacgct caagacacca cagcagacgc tgaagctgtg catcgatatc aatcgcctcg
     4801 agaacgaggc cgacgcgatc ctgcgccgct cggtagcacg gctgttcaag gacgaaaaag
     4861 atccgatcac cgtcatcaag tggaaggaaa tctacgaaag cctcgagaac gcctccgacc
     4921 gctgtgaaga cgtggcgaac atcatcgagg gcgtgatcct cgagcattcg tagaccatgg
     4981 acgcgacgac aatcgtctac ttaacggtcg cggtcgcgct ggtcttcgac atcatcaatg
     5041 gctttcacga cgcggcgaac tcgatcgcca ccgtcgtttc gacacgcgtt ctcacacctc
     5101 gactcgccgt gctgtgggcg acgctgttca acttcgtcgc gctgttcgtg ttccaccaag
     5161 gtgtcgcaaa cacgatcgcg aagatcatca cgatcgaagc atcggaccac gcgttcgtat
     5221 gggtcgtgat gtcgggactc gtcggcgcga tcgtgtggaa cctgctgacg tggtggtggg
     5281 gcttgccatc atcgagctcg cacgcgttga tcggcggcct ctctggcgcc ggcgtcgcgt
     5341 acggcggcac cgacctgctc aacaccgaca agctgtatcc gacgctccag ttcatcatcc
     5401 tcgcgccgct gatcggtctc gtgctcggcg gcctgatcat gttcatcgtg tactggctgt
     5461 tccgcggaat gcgaccgcga aaggtcgacc gcgtgttccg cgtcggtcag ttgttctccg
     5521 ccgccgcgta ctcggttggg catggcggca acgacgcgca gaagacgatc ggtgtgatct
     5581 gggcggtgat gatcgccggc ggtctgttgc cgaaggaggg cgcccacgtt ccctactggg
     5641 tgatcgtggc gtcgtacggc gcgatggcgg tcggcacggc attgggcggc cagcgcatca
     5701 tcaagacgat gggcatgggc atcacgtcgc tcaagccggt cggcggtttc tgcgcgaacc
     5761 tggcgggcgc gatcacgctg ttcggcgcga cgcacctcaa gattccggtg tcgacgacgc
     5821 acacgatcac cggcgcgatc gtcggtgtcg gatcggtgac caaggtgcgc ggcatccggt
     5881 ggggactggc cacgcggatc gtgtgggcgt ggattttcac gattccgctc gccggtctcg
     5941 tcggagcagc catgctgctc gccgcgcagg ccctgcacgc ctggtagcgc gcggctcgtt
     6001 gtcagggggc ggatttaggg tccctgccgt ggcgaagctc tacttccgtt acggcacgat
     6061 ggactcggcg aagtcgatga acttgctcgc ggtggcgcat aactaccgca agcaaggcaa
     6121 gaaggtgctg ctggtcaagc cgcgcctcga cacccggttc ggagcgacga agatttcgtc
     6181 gcgttgtggc ctcgaagcgg atgcagatct gatggtcgaa gaggacacgg ttctcgatcc
     6241 gcgtgacttc gcggatctgg actgtgtgct cgtcgacgaa gcgcagttcc tcgctccgag
     6301 catcatcgag gacctgcgcc gcatcacggt agatccgggc gtgcccgtga tctgctacgg
     6361 cctgcgcacc gattttcaga cgaagctgtt tcccggtgcg cagcgtctga tggagctcgc
     6421 cgaccgtatc gaagaggtga aggtcacctg tcagtactgc gcgaagaagg cgatctgtaa
     6481 cctccgcttc gtcaatggga cgccgaccgt cgtcggtccc cagatccagc tcggcgcgga
     6541 cgagcattac gcgccgatgt gctgggcaca ctacgacgaa gcgacgaagc acatcaccgc
     6601 ggagcagtcc ggcgcggtgc tcaggccctc gtagtacgta gctctagttt gcttatcagt
     6661 tcagatcggc gcggcgcgtg cgccactcgt tgaacagcgc gggaaaccgc gggtccgagt
     6721 ggagcggcga gaggtccgcg tcggtttcca tcttctcggt gtgctcgtat tcgtgctcga
     6781 tcgcgcgctg gacctgaacg agcgcgttgt cgacctgacc gatggccgca taggcgcagg
     6841 ccgccgagtg atagatgtac ggattctcgg gggcgtagcg ctgaccaccg tcggcgagct
     6901 cgaccgctag cttgtagtcg ccgagtgcgt gcgcgtgaat acacgcgttg ttccaagcca
     6961 tcacgagtgc cgtgcgcgct tcgccagcac cgcgcggtgg ctccggaagc gtgagcacgc
     7021 ggcgcagaat cgagagcgcg agtggccgat cgaccgagaa gctcgcgttg tagagatgtg
     7081 gaacgagcga gacgtcgtcg ggaagcttcg cgagcaagcc cgcgagctgg ttcggcgcgt
     7141 ggcgtgcgac ggatgcggtg aggtccgcga gctcctccgg ctcggtttca tcgagtgtcg
     7201 cctcgtcgag cagatcgagc gcgggcgcga ggtcattcgg tgcgtgctcc gcgatctcgc
     7261 cgatcacgag caggcgcacg tcgcggctac gcgcttgagt cgcgagcgaa atggcatcgg
     7321 cgagcctgcc ttggcgcacg agcacgatcg tgaggtagcc cgctgcatcc gagccggcat
     7381 cgaatgactg cgcgcgacga agcagccgct cggcgatcac ggcggtcgct ggattgtcgg
     7441 gatcgtgatc gagcgcgacc tcgacgagca tgccggcgat ttcgcggcga ctccacgagg
     7501 tctggccgac cgcccactgc agcgcggcct cttcgccctg gcgctggaac cgatcggcaa
     7561 acggattgcc ggcgagcacg aacggatcga tgccttcgcc accctgcgcc tgcgccttga
     7621 ccgcatcgtc gaccggctcg aagcgtgccc acgcgatcgg caggatgtcg tgcaggcgcg
     7681 cgacgatcca caataggaag tgcacctgat ccgatgcggt cgcgggcacc gcaaaccgct
     7741 cgacccacat cagtgcggag cgatgcttgc gatcgtgaat gacgtcggcg cgctggaacg
     7801 gctcgaattc ctcggagcgc tcgtcctgat acgccgatag ccagagcgca aagaacgcgt
     7861 tgatcaccga ctcctcgccg agcgacgccg tgccagcgag cttgaacgcg atgccaaagc
     7921 tctcccagtc gtagtcgtcg atcatttcgg gatagcgctc gatcggaaac ggcacgctgt
     7981 tctcgaacgg cggcggacca ttgcgccagt ggctctcgag cgtcacgacc tcgagctcgc
     8041 cgagcccttc gtcgtcgtct tcgtcgctgt cgtcgtcgtc gaccgtcgcc tcgggcggcg
     8101 gtgcatccgg tgcggcgtac agcacttctt ccgcgtcgtc gaataccgca tcgacgactt
     8161 ctgccggctc gctcgcctcg ggttcgtctt cgtcgctcgt cccctcgacg ctggccagct
     8221 cgagccacgc gatgccatcg acgccggtct cgactttgac cagccacacc gcgtcgatcg
     8281 aaaacggctc ggcggtggct tccagcgcaa cgcgctgtgg atcgacctcg tgggacgtcg
     8341 gcggcagcca cggtgcggcg tgccagtggc gcgtcggctc gctgtcggcg tcctcgtcgg
     8401 ctttctcggt cacgcccgca gcggacagtg cagcccacaa cgccttctgc gctgcggcat
     8461 cgagcgggcg cgtgctgcgc gcgatcagga tgcctcgcgc caggtcgctc cgccgcggct
     8521 cgagcccgac gatctccact tcgtccagcc tatctcaggc gacgagcgcg atcagcactc
     8581 gatgatgttt actgcgagac caccgcgcgc ggtctccttg tacttgctgc tcatgtcggc
     8641 gccggtgcgc cacatcgttt tgatgacctt gtcgagcgac accttgtgcg tgccgtcgcc
     8701 ctggagcgca agccgcgcgg cgttgatcgc tttgaccgag cccattgcat tgcgctcgat
     8761 gcatggcacc tggacgagcc cgccaatcgg gtcacaggtc agaccgagat tgtgctccat
     8821 gccaatttcc gccgcgttct cgacctgtgc gggcgttccg cccatcactt cggcgagcgc
     8881 gccggctgcc atcgagcacg cgaccccaac ttcgccctgg caacccacct ccgcgccgct
     8941 gatcgatgcg ttcttcttgt acagcgcacc gattgcggcc gccgtaagta gaaaacgcac
     9001 tgcgccttca tcatcggcgc ccggaatgaa gcgccggtag tacgtgagca cggcaggaat
     9061 gatgcccgct gcgccgttcg tcggtgccgt cacgacacgg ccgcccgcgg cgttctcctc
     9121 gttgaccgcg agggcgaaca aattcaccca gtccatgatg acgagtggat cgcgatcgcc
     9181 gcgcggatcg ctcttgagct tgcgaaacaa tgccgccgcg cgccgcttca ccttgagacc
     9241 accgggcaag atgccctcgc gcttgcagcc gcgctcgacg cattgatcca tcgcgatcga
     9301 gatctcgagc acgcgccggc ggatctccgt ctccggcagg agcgccttct cgttctcgag
     9361 catcagcgac gaaatcgcca tgccgtgctc acccgcatga cgcagcagct cttcgccgga
     9421 attgaacgga tacggcacct ggacctggtc ggccggtgtc gagccgtcgg caggtgcgcc
     9481 ggcgtgatcg acgacgaagc caccaccgac cgagtaatag atgcgctcgg cgagcggtgc
     9541 gttcgcgccc gccggcccga gtgccgagaa ccgcatgccg ttcgcgtgca gcggcagctt
     9601 ctcgcggcgg tgaaacacga ggctcgttgc cggatcgaga tcgacttcgt gcttgccgag
     9661 cagcttcacg cgcttgctct gctcgacgac ggcaacgcgc cgcgcgaccg cgtcgacatc
     9721 gacagtcgcc gggtcttcgc cttcgagccc caagatgatc gccttgtcgc tgccgtggcc
     9781 tttgccggta aatccgaggc tgccgaacaa ctcgatcttc acgccttcga cctgcgctag
     9841 cacgccgtcg atctcgagcc gctcggcgaa ccggcgcgcg gcgcgcatcg gcccgaccgt
     9901 gtgcgagctc gacggcccga tgccgatctt gaagatgtcg aagacggaga tgtgcacggc
     9961 tcaggcggag tatggcctga aaccgctgct agaacagaat cctagtcttg atactggttt
    10021 ccagcccggc cacggccttc cgcacatcct gcgagacgtc ctgatcgagg tccatgatca
    10081 ggtagccaat gtccgggtcg gtcgcgagca actgggcgcg gatgttcgcg ttgagatcgc
    10141 tgacgatctt gttgatgtcg cgaagcacgc cgggaacgtt gcggtgaatg ttcaagatcc
    10201 ggtgcgcgcc cctcgtgatc gggagctcga ccggcgggaa gttgaccgcg ccagcggtcg
    10261 tgccggtgtt gatgaacttg atcagcgcgg ccgacacttc cttgccgatc gcttcctgcg
    10321 cctcttccgt cgagccgccg atgtgcggcg tcaaaatcac gttgccaagc ccgcgcagcg
    10381 gtgacgcgaa gccatcgctg ttcgtctccg gctcctcggg atacacgtcg accgccgcac
    10441 caccgagatg cccgctcttc agcgccgccg cgagcgcatc gatgtcgacc accgtgccgc
    10501 ggctcgcgtt gagcagacac gcgcccttct tcatgcgcgc gatctgctcg tggcccatca
    10561 tcatcttcgt ctgcggcgtc tccggaacgt gcagcgtgac gaagtcactc tcggccagca
    10621 cgtcctcgag cttcggcagc gaccggttgt tgcccatcgg cagcttcgca gcgatgtcat
    10681 agaacatcac gcgcatgccg aacgattcgg cgagcacacc gacctggctg ccgatgtggc
    10741 cgtagccgat gatgccgatc gtcttgccgc gcacctcgtg gctgccgacc gcgaccttct
    10801 tccattgtcc cgcatgcact tcgcgcacgc gatcgccgag gtgacgcgac agcatcacga
    10861 tctcggcgat gatcagctcg gcgaccgagc gcgtgttcga gaacggcgcg ttgaacacgg
    10921 gagtgccgcg gcgattcgcg tggccgagat tgatctgatt cgtgccgatg cagaacgcac
    10981 ccaccgtgag caaacggcgc gcctcgtcga gcgccctcgg cgtgacctgg gtcttgctcc
    11041 ggatgccgag gacgtggacg tctctgatct tgtcgacgag ctccgcctcc gagagcgcgc
    11101 ccttgacggt ctcgacttga aagccctccg ccgcgaactc ctccttggcg acgggatgga
    11161 tgttctcgag gaggaggatc ttgatctcgg acttcggaaa cgacgtctcg gccatgcgag
    11221 ggggaattta gcaggataga gtcccgcgca tggccaaagc gaagaccgca gccccgaaga
    11281 aagcggccgc gccgcgtaag aagaaggtga aggccgctgc aggctcggtg ggccttactg
    11341 ccgacgaagt cgcgtcaggt tcaccgccgg ccgaggtcac gaagctcgag aaacagatcg
    11401 aagctgcgga cggc
//
LOCUS       DASUFX010000722         7944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1921441, whole genome shotgun sequence.
ACCESSION   DASUFX010000722 DASUFX010000000
VERSION     DASUFX010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921441"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1035)
                     /locus_tag="VFV99_29245"
     CDS             complement(91..1035)
                     /locus_tag="VFV99_29245"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEX5063512.1"
                     /translation="MMRTLLLSCCLLAGCIVGDEREPETGTLAATWGPYDPQPGHPTQ
                     ADRDAFVAQISQYAEEGEQRYGTPAADIIAMACNESGFGWTRIALNANNLFGYKWTSY
                     AMAGGRGFWTLEDQPADDPGNQYIDFADRRDAVLYVAQRLATSTRYKPTTDQYSADIA
                     NGMDVKSAADRWIYGIASAGYNPYEHYPVTTIKFMNNYRTPSLTRSPTYDLYRYSAEA
                     APAAWVSIDAPAANAYVAGNVTLTSHASASTTSVQFASRAKGASDWYSLGAATTSPFM
                     RTWATSGYVPNGQYELRAQAYRGTTLVATGVITVTVAN"
     gene            1251..2864
                     /locus_tag="VFV99_29250"
     CDS             1251..2864
                     /locus_tag="VFV99_29250"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX5063513.1"
                     /translation="MIAACGGAGGNPGGGGDDDDDMDAMLVDGSTPDAMRPLSTPVMP
                     LGGDDGFYPRAIALGDGTIIAAVVAPQPSGRLGGTIRESIDGGQTFEVVGRVDGQFSI
                     GGLCCATLYELPAPLGALPAGTLLWSASVGGDNPNAPMSIPVYASTDRGRNWSFLSNV
                     VIASKPRSQGGLWEPEFAQLADGSLICHYSDETDSAHSQKLVARRTTDGVTWSASRDI
                     VSLAPFAARPGMAVVRKSPAGPYVMTFEICGTDGCATHLRMSADGWNWGNANDVGLRP
                     ETLDGKHFRHAPTLAFSPTPGANGRFYLIGQMLYDRNGVVTGGNGAVILANTEGGYGR
                     WYEVPAPVPVTNAYDNFCPNYSSAILPVDGGLAVLEIASKYDGNRCRPYFARGPLRGT
                     GDATGLADGASYRMTALVSGFCVDVINGLTTAGANIRQWDCNGANAQRFRIAMQPDGT
                     AQLVNVGSGMCATAVGNPNDPGANVEQQPCTGASRWKLANVGMGYYVAAHAGTGSCLD
                     VAGGSGTMGANIQQWTCNDLSPQIWRFDP"
     gene            complement(2861..3310)
                     /locus_tag="VFV99_29255"
     CDS             complement(2861..3310)
                     /locus_tag="VFV99_29255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063514.1"
                     /translation="MALVVVVAACASRSVSPTSIATATPAPTTTAAPAKKKVTVTTET
                     HVDILDPIKFLPGSATIEVTSIPMLDAVARTLAGNPSIKLVAVQAFGADTVVRFQAQL
                     ATTRAQTIVDELVARGVEAKRLVAEGNAQPPAGYPNAPIFLILERTP"
     gene            complement(3350..4525)
                     /locus_tag="VFV99_29260"
     CDS             complement(3350..4525)
                     /locus_tag="VFV99_29260"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063515.1"
                     /translation="MRGFACAALVLVACGRLRFDPISGADGDGGIGDGSGSAGPDAPL
                     AGFSHVVAAHNQTCALYRDNKLYCWGNSPSGEVGVGVFGQVLVPTPYILPAGHVDDFS
                     TGGNRSCAIVDGTVYCAGNMESPAQSTPKPYPTPFPATRIGAGDNFVCALSAGAVYCW
                     GDNDLAQLGVGDTGVHASWQRLAFTGDPTIVALEVGDKHACAIDTTNTVWCWGDNNHG
                     SLGTGSTSPAVVAIPTKTLATIRTLPQIAGWHACSLEAGIVRCWGEGDNGELGDGNTN
                     NSATPVTIPTLSGVTVLSTGGGVNEFDASCVSVAGDVKCWGLGMRLGAGNTSPSSVPV
                     TVNGLPGPAVELALGNEHACARLVDGDLWCWGRGVHGQLGDGLQTTSANPVLVVRPP"
     gene            complement(4567..4989)
                     /locus_tag="VFV99_29265"
     CDS             complement(4567..4989)
                     /locus_tag="VFV99_29265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063516.1"
                     /translation="MVPAPKSDAVADLIARANELADGQRQAAIELLTKARKTYPDDAR
                     LPYHLALLYLDKMWWADGLKQARAAIALDPKLRSDPDLIKLVLKGFNTTKNYDWTLAK
                     FLREDVGDAAKPYMEDTAKNHPNPIVRQRATAELRRYK"
     gene            4999..5970
                     /locus_tag="VFV99_29270"
     CDS             4999..5970
                     /locus_tag="VFV99_29270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063517.1"
                     /translation="MLLRQHAFEAPPTLQQVAPTQTFAPELEAFVARALAKQPDDRYR
                     SAADMNAALDAVVQVLAQPARVSAPDVVATPRTVTDQRLLETEPVRGSRRRPLLIGGV
                     AAALATVVVIVVALRGGSKPTPGELAVKATTLAQTGSNQAAVDLLERELVGRATAEQA
                     PAYLALGHARFKLGRTQQALAAYERALRAEPSAGGDPELRANLLKVLDGNGSLASVVA
                     LDLLATLTPPASDAIAQYAATGKVDDARHRALMIAERDGITVDRVQSWLLDLERAPSC
                     EDRKATIELLARSSDRRALAGLKRARMLKCVEREATDAMARIEATAK"
     gene            6238..6687
                     /locus_tag="VFV99_29275"
     CDS             6238..6687
                     /locus_tag="VFV99_29275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063518.1"
                     /translation="MCDAATPPKELANLVEVFRLALAVHSATEAHVLDLLLTRVGGPR
                     TLSMIVTHTRIEHSAQRVAAERLLQIRPASLAWYEHAIALRDLVLDHASRSDRARWTF
                     NDHLPAELHEALATEYARERMRVLGCTSPLDLAQRRDHRVPFNRADA"
     gene            6762..>7944
                     /locus_tag="VFV99_29280"
     CDS             6762..>7944
                     /locus_tag="VFV99_29280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063519.1"
                     /translation="MAIDDEWAGSERPDPSGLVGKMAGRYRIVSEVATDGRRTLYEAE
                     NGDGERVTIGVVRADGTPATERLRNGEKLRSLKQPGLVSVLDVGKLDGGDLWVANERA
                     VGATLRTMITGTLLEQRRALAIIRQVLEALAAVHAAGAIHGGITPESIHVAVEVALDG
                     RVDRVKLTDFGLATLAGSAKVGDGRYAAPETALGKVDGRADLYAVGAVLFELLTGHPP
                     FFANDAEALRRLHAYGPLQTLAQRAPGVTFVPALEDVVARALAKKREARYQTADEMIE
                     ALDRAIEAVEAAAPPPPEPDGGRRRKPNDSLLVLAKDLMPASQATNDVVIPVNVDRRV
                     PELPLSLRAKKRARSALSPFRARFDKLSRRQKQIVAAAAAVVVLIVIIVIALSGGGKQ
                     SH"
BASE COUNT         1296 a         2610 c         2692 g         1346 t
ORIGIN      
        1 ccaggcgtca taaggtagtg aaggccatga gcgattcatg gtcgttagcg ctgcagattt
       61 tctgccaagc ggcgcgtgcg cagatttctg tcagttcgcc accgtcacgg tgatcacgcc
      121 ggtcgcgacc agcgtcgtgc cgcggtacgc ctgcgcccgc agctcgtact gaccgttcgg
      181 aacgtaacca ctcgttgccc acgtgcgcat gaacggagac gtcgtcgcag cgccgagcga
      241 gtaccaatcg ctcgcaccct tcgcgcgcga cgcgaactgc accgatgtcg tcgacgcgct
      301 cgcgtgcgat gtcagcgtga cgttgccagc gacgtacgcg ttcgccgcgg gcgcatcgat
      361 cgagacccac gcggctggcg ccgcttccgc cgagtaccga tagaggtcgt aggtcggcga
      421 acgggtgaga ctgggtgtgc ggtagttatt catgaacttg atcgtcgtga ccgggtagtg
      481 ctcgtacggg ttgtagcccg cggatgcgat gccatagatc cagcgatcgg cggcggactt
      541 cacgtccatg ccgttcgcga tatcggcgct gtattgatcg gtcgtcggct tgtaacgcgt
      601 ggacgtcgcg agcctctgcg cgacgtagag caccgcgtcg cgacggtcag cgaagtcgat
      661 gtactggttg cccggatcgt cggcgggttg atcctcgagc gtccagaacc cgcggccacc
      721 cgccattgcg tacgacgtcc acttgtagcc gaacaggttg ttcgcgttga gcgcgatgcg
      781 cgtccagcca aaaccacttt cgttgcacgc catcgcaatg atgtcggcgg caggcgtgcc
      841 gtaacgctgc tcgccttcct ccgcgtactg actgatctgg gcgacgaacg catcgcgatc
      901 ggcctgcgtc ggatgaccgg gctgcggatc gtacggcccc cacgtcgcgg caagagttcc
      961 ggtctcgggt tcgcgctcgt caccgacgat gcagcccgcg agcagacaac acgacagcag
     1021 cagcgtacgc atcatccgcc gagcgtagtc gcgcagctcg tgcgtccgtg agtgacgacc
     1081 actggcggaa actgtcactg cgaacgaacg aatccgtgtg caactaacag tgctcagacc
     1141 ggagacatca cccatcgaaa ccgccaaagt cgggcacgcc actggcgcac tgcctggcac
     1201 atcgaatgcg taacgctatc gcgatggtac gagttggatt cgttttgtcc gtgatagcgg
     1261 catgcggcgg agcgggaggc aatcccggcg gcggcggcga cgacgacgac gacatggacg
     1321 cgatgctcgt cgatggcagt acgcccgacg cgatgcgtcc actgtcgacg ccggtgatgc
     1381 cgctcggagg tgacgacggc ttctatccac gcgcgatcgc gctcggcgac ggcacgatca
     1441 tcgcggcggt ggtcgcgccg cagccgagtg gccgactcgg cggcacgatt cgcgagagca
     1501 tcgacggcgg tcagacgttc gaggtcgtcg ggcgcgtcga tggtcaattc tcgatcggcg
     1561 gtctgtgctg cgcgacgctc tatgaattgc cggcgccgct cggtgcgttg ccggccggca
     1621 cgctgctgtg gtctgcatcg gtcggcggcg acaacccgaa cgcgccgatg tcgattccgg
     1681 tctacgcgag caccgatcgc ggtcgcaact ggagcttcct gtcgaatgtc gtgatcgcga
     1741 gcaagccgcg gtcgcaaggc ggcttgtggg agcccgaatt cgcccagctc gcggacggct
     1801 cgctgatctg tcactactcc gacgaaaccg acagcgcgca cagccagaag ctcgtcgcgc
     1861 gtcgcactac cgatggcgtc acgtggtcgg catcgcgcga catcgtctcg ctcgcaccgt
     1921 tcgcggcgcg gccgggcatg gcggttgtac gcaagtcgcc cgctggtccg tacgtgatga
     1981 cgttcgagat ctgcggcacc gacggctgcg cgacccacct gcgcatgtcg gccgacggtt
     2041 ggaactgggg caatgcgaat gatgtcggcc tgcgccccga gacgctcgac ggcaaacact
     2101 tccggcacgc accgacgctc gcgttcagtc cgacgccggg cgcgaacggt cgcttctatc
     2161 tgatcggcca gatgctctac gaccgcaacg gcgtcgtgac cggcggcaac ggtgccgtca
     2221 tcctcgcgaa caccgaaggt ggctacggtc gctggtacga ggtcccggca cccgtgcccg
     2281 tgacgaacgc gtacgacaac ttctgcccga actacagctc ggcgatcttg cccgtcgatg
     2341 gcgggctcgc tgttctcgag atcgcatcga agtacgacgg taatcgctgt cgcccgtact
     2401 tcgcgcgcgg accgttgcgc ggcaccggcg acgcgaccgg tctcgccgac ggcgcgagct
     2461 atcgcatgac ggcgctggtc agcggcttct gcgtcgacgt gatcaacggc ctgacgacgg
     2521 ccggcgcaaa cattcgccag tgggactgca atggcgccaa cgcgcaacgc ttccgtatcg
     2581 cgatgcaacc cgatggcacc gcgcagctcg tcaacgtcgg cagcggcatg tgcgcgacgg
     2641 ctgtcggcaa cccgaatgac ccgggcgcga atgtcgagca gcagccgtgc acgggcgcga
     2701 gcaggtggaa gctcgcgaat gtcggcatgg gctactacgt cgcggcgcac gccggaacgg
     2761 gctcgtgtct cgacgtcgcc ggtggttcgg gcacgatggg cgcgaacatc cagcagtgga
     2821 cgtgcaacga tctctcgcct cagatctggc gcttcgatcc ttagggcgtg cgctcgagga
     2881 tcaaaaagat cggcgcgttc gggtaacccg caggcggctg cgcattgcct tcggcgacga
     2941 gacgcttggc ctcgactccg cgtgcgacga gctcgtcgac gatcgtctgc gcgcgcgtcg
     3001 ttgcaagttg cgcctgaaat cgtacgaccg tgtcggcacc gaacgcctgc acggcgacga
     3061 gcttgatgct cgggttgcct gccagcgtgc gtgcgaccgc gtcgagcatc gggatcgacg
     3121 tcacctcgat cgtcgcggag ccgggcagga acttgatcgg atcgagaatg tcgacgtgcg
     3181 tctcggtcgt gaccgtgacc ttcttcttcg caggcgcggc ggttgtcgtc ggcgccggcg
     3241 tcgcggtcgc gatcgacgtt ggcgacacgc tacgactcgc gcacgcggcc accacgacga
     3301 caagtgccat ccaccgcacg catcgatcgt agcaggtgat cttcgcgatt cacggcggtc
     3361 tcacgaccag caccgggttc gcgctcgtgg tctgcaagcc gtcgccgagc tggccgtgca
     3421 cgccgcggcc ccagcaccac agatctccgt cgacaagtcg ggcacatgca tgctcgttgc
     3481 cgagcgcgag ctccacggcc ggacctggca ggccgttgac cgtgaccggc accgacgagg
     3541 ggctcgtgtt gccagcaccg agtcgcatgc cgaggcccca gcacttcacg tcgccggcga
     3601 ccgagacgca gctcgcgtcg aattcgttca cgccgccgcc agtggacagc acggtgacgc
     3661 cggagagtgt cgggatcgtc accggcgttg cagagttgtt cgtgttgccg tcaccgagct
     3721 cgccgttgtc gccttcgccc cagcacctga cgatgcccgc ttcgagcgag cacgcgtgcc
     3781 agccggcgat ctgcggcagc gtgcggatgg tggcaagcgt cttggttggg atcgcgacga
     3841 ccgcgggtga cgtcgagccg gtgccgaggc tgccatggtt attgtcgccc cagcaccaga
     3901 cggtattggt cgtatcgatc gcgcacgcgt gtttgtcacc gacttcgagc gcgacgatcg
     3961 tcggatcgcc cgtgaacgcg agccgctgcc agctcgcgtg cacacccgtg tcgccaacgc
     4021 cgagctgcgc gaggtcattg tcgccccagc agtagacggc gccggcggag agcgcgcaga
     4081 cgaagttgtc tccggcgccg atgcgggtgg cgggaaacgg cgtcgggtag ggcttcggcg
     4141 tcgactgtgc gggcgactcc atgttgccgg cgcagtacac ggtgccgtcg acgatcgcgc
     4201 acgagcggtt gccgccggtc gagaaatcgt cgacgtggcc ggcgggcagg atgtatggcg
     4261 tggggacgag gacctgaccg aagacgccga cgccgacttc accactcggg ctgttgcccc
     4321 agcagtagag cttgttgtcg cgatacagcg cgcaggtctg attgtgcgcg gcgacgacgt
     4381 gcgagaagcc ggcgagcggt gcatcggggc cggccgagcc ggaaccatcg ccaatgccgc
     4441 cgtcaccgtc ggcgccgctg atcggatcga aacggagccg gccgcacgcg accagcacca
     4501 gcgccgcaca tgcaaaccct cgcatcaccc cgattcaatc tacacgatcg tcgcgtgcag
     4561 ctgacgttac ttgtagcgac gcagctcggc ggtcgcgcgc tggcgcacga tcggattcgg
     4621 gtggttcttc gccgtgtcct ccatgtacgg cttggcggca tccccgacgt cctcgcggag
     4681 gaacttcgcg agggtccaat cgtagttctt cgtcgtgttg aagcccttga gcacgagctt
     4741 gatgagatcc ggatcgctgc gcagcttcgg atcgagggcg atcgccgcgc gcgcctgctt
     4801 gaggccgtcg gcccaccaca tcttgtcgag atagagcagc gcgaggtggt acggcaggcg
     4861 ggcatcgtcg ggatacgtct tccgcgcctt ggtcagcagc tcgatcgcag cctggcgctg
     4921 gccatcggcg agctcgttcg cgcgcgcgat caggtcggcg accgcgtcac ttttcggcgc
     4981 cgggaccaag atcctgcggt gctgttgcgg cagcatgcgt tcgaggcgcc gccgacgcta
     5041 cagcaggttg cgcctacgca gacgttcgcc cccgagctcg aagcattcgt ggcgcgtgct
     5101 ctcgcgaagc aaccggacga tcgctatcgc tcggccgcag acatgaacgc tgcgctcgat
     5161 gcggtcgtgc aggtactcgc gcagccggcg cgggtgtctg cgccggatgt tgtcgcaacg
     5221 ccgcgaaccg tcaccgatca gcgactactg gagaccgagc ccgtgcgcgg cagtcgtcgc
     5281 cgcccgctgc taatcggtgg cgtcgccgcg gcgctcgcga cggtggtcgt catcgtcgtc
     5341 gccctgcgcg gcggttcgaa gcccaccccc ggcgagctcg ccgtgaaagc gacgacactc
     5401 gcgcagactg gcagcaatca ggcagcggtc gacctgctgg agcgcgagct cgtcggacgc
     5461 gcgaccgccg agcaagctcc cgcgtatctc gcgctcggcc acgcacgctt caagctcggc
     5521 cgcacgcagc aagcactcgc cgcgtacgaa cgagcgcttc gggctgagcc gtcggccggc
     5581 ggggatccgg agctgcgcgc caacctcttg aaggtgctcg acggcaacgg ctcgctcgcg
     5641 agcgtcgtcg cgctcgacct gctggcaacg ctgaccccgc cggcgagcga tgcgattgcg
     5701 cagtacgcgg cgaccggcaa ggtcgatgac gcgcggcatc gtgcgctgat gatcgccgag
     5761 cgggatggca tcacggtcga ccgcgtccag agctggctgt tggatctcga gcgcgcaccg
     5821 tcgtgcgaag atcgcaaggc gactatcgaa ttgcttgccc gctcgtcgga ccggcgtgcc
     5881 ctcgccgggc tcaagcgcgc ccggatgctg aagtgcgtcg agcgcgaggc gaccgacgcg
     5941 atggcccgga tcgaagcaac cgccaagtag tgatctttgg cgcaaccgat tactgcgccc
     6001 cgaagctggg cgattccgcc acgtgcgggt tttgcacgcg tggcaaaagc gcgcggtttg
     6061 tcgtcgagaa acacgttccc aagtcctcga tctcggtccc ggcgtggccg cgttcgtagt
     6121 cgacgctaca aacgggcctt cggcactgta gcagcatgtc tcgctacccc aatgggcctt
     6181 gatttgtgcg gatgggtgcg acgggaccac gacgatctcg accgcgcact gagttgtatg
     6241 tgtgacgcgg cgacacctcc caaggagctg gcgaacctcg tcgaggtatt tcgcctggcg
     6301 cttgccgtcc actcggcgac cgaggctcat gtcctcgacc tcttgctgac gcgcgtcggc
     6361 gggccgcgca cgctcagcat gatcgtgacg cacacgcgca tcgagcactc agcacaacgc
     6421 gtggctgcgg agcgcttgct ccagatccgg ccggcgagcc tcgcgtggta cgagcacgca
     6481 attgcgctgc gtgatctcgt gctcgatcat gcgagccgct cggaccgcgc gcggtggacg
     6541 ttcaacgacc atctgccggc ggagctccac gaagcgctcg ctaccgagta cgcccgcgaa
     6601 cgaatgcgtg tgctcggttg cacctcgccg ctggatcttg ctcagcgtcg cgaccatcgc
     6661 gtcccgttca atcgcgctga cgcctagtca ttgcgcagtt aggcgcgtag gcgtactccc
     6721 acatcgtcgg gccccggagg tcttgtaaga tcgttggtcg aatggcgatc gacgacgagt
     6781 gggcggggtc tgagcggcct gatccgtcgg ggctggtcgg caagatggcc ggccggtacc
     6841 ggatcgttag cgaggtcgcg accgacggtc ggcgcacgct gtacgaagcc gagaacggcg
     6901 acggtgaacg cgtgacgatc ggcgtcgtcc gtgcggatgg cacgcccgcg acggagcggc
     6961 ttcgcaatgg cgagaagctg cggtcattga aacagcctgg gctcgtcagc gtgctcgacg
     7021 tcggcaagct cgacggcggc gacctctggg tcgcgaacga acgcgcggtc ggcgccacgt
     7081 tgcgcacgat gatcacgggc acgctactcg agcagcgccg cgcgctcgcc atcatccgcc
     7141 aagtgctcga ggcgctcgcc gccgtgcatg cggccggcgc gattcacggc ggcatcacgc
     7201 ccgagagcat ccacgttgcc gtcgaggttg cactcgacgg tcgcgtcgac cgcgtcaagc
     7261 tgacggactt cggcctcgcg acgctcgcgg gctcggcgaa ggtcggcgat ggacgctacg
     7321 ctgcgccgga gaccgcgctc gggaaagtcg atggccgcgc agatctgtac gcggtcggcg
     7381 ccgtattgtt cgagctgctt accggccatc cgccgttctt tgcgaacgac gccgaggcac
     7441 tgcgccgatt gcacgcgtac ggaccgctgc agacgctcgc gcaacgtgcg cctggcgtga
     7501 cgttcgtgcc tgcgctcgaa gacgtcgtcg cgagggcgct cgcgaagaag cgtgaggctc
     7561 gctatcaaac ggctgacgag atgatcgaag cgctcgatcg cgcgatcgag gcagtcgagg
     7621 cagcggcacc gccaccgccc gaaccggacg gtggacgccg gcgcaaaccg aacgactcgc
     7681 tgctggtgct cgcgaaggac ctgatgcctg cgtcgcaagc gacgaacgat gtcgtcatcc
     7741 cggtcaacgt cgatcgaagg gttcccgagt tgccgttgtc gttgcgcgcg aagaagcggg
     7801 cgcgcagcgc gctctcgccg ttccgcgcgc gcttcgacaa gctgtcgcgg cggcagaaac
     7861 agatcgttgc ggccgctgcg gcagttgtcg tcttgatcgt gatcatcgtc atcgcgctgt
     7921 caggtggcgg caagcagtcg cacg
//
LOCUS       DASUFX010000723        12290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1645917, whole genome shotgun sequence.
ACCESSION   DASUFX010000723 DASUFX010000000
VERSION     DASUFX010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645917"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFV99_29285"
     CDS             complement(<1..243)
                     /locus_tag="VFV99_29285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063520.1"
                     /translation="MAISIDAPENRVSDGVICLAPFELADVVTVMKWDADPTVQHWYD
                     WPLTPPWNDPDTYEARRAHAERVVRDQPAAWDAGEQF"
     gene            complement(324..1619)
                     /locus_tag="VFV99_29290"
     CDS             complement(324..1619)
                     /locus_tag="VFV99_29290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5063521.1"
                     /translation="MRKTVFAILLVVAAACDQGKSKLDEMPAAKTTTGSGATTSKGGD
                     AIDIDSKDILNRKEATDVVQVKHVLIGWKDLDEVYQGRLDPRAAKRTNADAAKLAKEI
                     ADKLRKDPDSIDALIKEHSEDPGSLSGEPYEVKADAPFVPEFKALAVHLKEKEVGIVK
                     TKFGYHVMERVPPPPPDPLESADILKREPPEKGTVHVQHVLIGWKDAPAAKSGRGDPR
                     AKDRTKAEADKMAQDILARAQKGEDMTKLMKDFSEDPGSKDNGRAYPVSPDAPMVEPF
                     KNLSLRLKEGEVGLVKSPFGWHIIKRIPPPPPDPLESADILKREPQTQKAKVKHILLG
                     WSEVNAGGDERAKKRTRPQLEKLVKDTVAKLQKGSKIEPLMAELSEDPGSAKSGEGYD
                     VTPDAGLVEPFKNLSLRLKVGETGVVKTDFGIHIIQRVE"
     gene            complement(1738..3063)
                     /gene="eat"
                     /locus_tag="VFV99_29295"
     CDS             complement(1738..3063)
                     /gene="eat"
                     /locus_tag="VFV99_29295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HEX5063522.1"
                     /translation="MTQLQKTLGPVLIWGLGVGYVISGMYFGWNLGLPEGGPYGMLVA
                     TGVVTILYVTFVLGYAELACALPKAGGVFVYTSRAFGPHLGFIGGVAQLVEYMLAPPA
                     IAFAIGSYINQTIPSIPIPVIAVAAYVVFTAINIWGMKLSVTFELVLTVVAVIELCVF
                     GSVALPSFSWDNFSADALPHGWGGAFAALPFAMWFYLAIEGIANVAEEAKDVQRDLPR
                     GFLWAMATLVALTAVTLFGAVGAAGWQAVVYPDPANHAVTTDSPLPMAIGHIVSRESP
                     LFVVLTGIGLIGLIASFHGILIAASRAILEFGRARYAPAIVGEINARTKTPVVALLVN
                     LGVGMIALATGKTSDIILIAVFGALTLYSLSSIAVLRLRMREPELPRPYRTPGYPFTP
                     WIALALSLLCIVAMIVRYPTLALVYAAILVGAWVLFLVFVPADRRSSIV"
     gene            3157..5598
                     /locus_tag="VFV99_29300"
     CDS             3157..5598
                     /locus_tag="VFV99_29300"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5063523.1"
                     /translation="MTPPALPSRARVVIVGGGIIGCSVAYHLAHMGWKDVVLLERDRL
                     TSGTTWHAAGLIVTFGSLSETSTEMRKYTRDLYAKLEAETGQDTGFKPVGFIEVAADK
                     DRLEEYRRVSAFNRICGVDVHEISPGQVRELFPIAKTDDILAGFYVKEDGRVNPVDAT
                     MALAKGARMQGAKLIENVPVTGFLTKRGAVTGVRTAYGDIEAEFVVNCAGMWARQIAR
                     EVGVNIPLQAAEHYYLITERMKQISPDWPVLEDPGSYGYFREEVGGLMIGLFEPKCAP
                     WNVGGIPGDFSFGEIQPDWERMGPYVEKAMARVPISMETGVKKFFCGPESFTPDLAPI
                     VGEAPELENFFVCAGLNSIGIITGGGMGRLMAHWIINGRPDQDISAIDIARLHGYQSN
                     PEYRRLRTVESLGMVYQCHYPFRNLETARGAKKSPFYDRLAARGAYFRDVSGWEGADW
                     YGCDDNTLTFKRPPWFPQWQREHTAAREGVIVMDMSFMSKFLVEGRDAGRLLNWISAN
                     NVDGQTGQITYTQWLDEAGKLQGDLTVTKLADEKYWVVASDTVHRHTQWWMKRHFGDE
                     HAFVADMTAAYAQLNVQGPRSRELMQLVTTTDMSNDAFPFRAAKEIDIGFARVLCIRI
                     TYLGELGYELYIPTEQALHVYDRLVEAGEKVGLVHAGLKTLASCRMEKGYRDMGHDID
                     NTDTVLEAGLGFAVDLKKPGGFLGKDAIVAQKAAGPLKKRLLQILLKDPEPLMFHAEI
                     VKRNGKPVGYIRAASYGHTLGGAVGLAMIEAPEPIDQKWIDAGAWEVEIANQTYPAVA
                     SLKPLYDPENKKVKL"
     gene            5608..6750
                     /locus_tag="VFV99_29305"
     CDS             5608..6750
                     /locus_tag="VFV99_29305"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063524.1"
                     /translation="MVARGPGDVGCAHCTLIGADRPCPVCTHLVCERCASDWTTCSEP
                     SGRVIRLGLTARVRDVDPSGRLALVSHWRQPLRVFDLRQLRWVRDIRLPRGAYLWNRT
                     RPPRLTSDGRFIHAVWGAGTGYERTDTLVFKGMRFDKLGSNNTELVPSAEVPDHGTAV
                     SPTRDVFYYVTDTQRVALIDGGRAVSVEPLPRKVVHSVYFDAERELLACGGWKEIVLD
                     HTVDGHLSRVTRIETRNTGDVHWLALAGGWLVAAIWVSGTSAIEIRELSDNLAIGPVR
                     HRHVTTRLRAAALSRDGRYFAFGDYNGLVVHELATDRTTVFTEHSDEINFVSFSRDDH
                     TLISADTDNRVVMRPRTEAGYAKPVIAIDVPSEGVDLPRFQPRAVP"
     gene            6957..7901
                     /locus_tag="VFV99_29310"
     CDS             6957..7901
                     /locus_tag="VFV99_29310"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063525.1"
                     /translation="MMRRYTLALLLAFGCGGTNPMGGDDVQPPMDGNGSNGGDADTFQ
                     PPAGYTKLIGRTWTLAAGQHDIYKCVRIHVPTDTYITSIIAQAPPGTHHTVLSIASSL
                     GGSAGPDGEYDCEVRTLGLNMLYASGVGTSPLDFPTDVGVKIAAGTQIHLNLHLYNAS
                     DDTLSGDSGIWVKSQPTAPPMLAEMQFAGPLDLTIPKMSTNYPITSTCTINNSYQLFA
                     LWPHMHKHAVHQKIELLRGTAQPVTLHDMPYQFGEQKYWPQSPEIQLQPQDKIRVTCS
                     YDNPTNADINWGDSSDEEMCFGGMYRYPSQGSILFCQN"
     gene            7984..9693
                     /locus_tag="VFV99_29315"
     CDS             7984..9693
                     /locus_tag="VFV99_29315"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063526.1"
                     /translation="MLGGVLAAVALVSASGCFKGEFANCKVTCATSADCPGSLECNPQ
                     GVCSPGGDMCSAMPDADLSKPQTLTVSTMGDGAVTSDPAGIDCGATCMHDFPPLSSVT
                     LTAAPAGTSVFDGWTGDCAGQAETCSLTMDGPKSATAKFATHGALRFVKQISFSGQDF
                     VGSELAVDSNGDVIATGSIDDQGAPGFYTVKYGKDDGHIIWEKKTIVSGGFFGIGGLT
                     TDSSGNAYMCTRFQGQGAATIGPSTVTGDLFGNVLVVKYAAADGNIEWAKSWGGSAQE
                     ECAGIVASGTDIFVTGYTSSNPSNFDSQVLAGSVNNGFVVRAVQSNGQAQQAILLGAN
                     FQIYDIAENQGQIAVVGSFTASNYITGSCNFTSTATGTDGFIMNMSSNLVCTWNKTFG
                     SLTANQNTASFAVADVPGGGWAVTGYFEGSVNFAGTGSAVGNQGGRDAFIARYAGNGT
                     HIYSFGYGAAGNDYGRGVDVTPTGEVVFGGEFQNTVTFGATMLTTTNQDVFMTRLSAG
                     NTPTHQWAAKVGGTASEQVQGLKVDAQGTVSVLAYWTGMTDIGGMQLTAADYDAFVGS
                     FVR"
     gene            complement(9703..10515)
                     /locus_tag="VFV99_29320"
     CDS             complement(9703..10515)
                     /locus_tag="VFV99_29320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063527.1"
                     /translation="MKRFVAITFVMLWTAVASAQQKPDVSALETSGNKHFELAEYDAA
                     ISDFKEAFRISDEPGYLYNIAQAYRLKRDCREAATFYKTYLRRVPKAPNAAKVRERIT
                     EMEKCAETQPATPPPVTTTPTTTTPTTTTPTTTTAQPEPLEKPEDEERPETPPAANPR
                     GWMKWAGIAAIGVGALGGGLAIKFALDGKSANDDLAKLCETSCTGADAKALESDGNAA
                     NRNAVIASVAGGAFLATGVVFLVLSRSGATQQPEVSLQLTRGGAAAGYAWRF"
     gene            complement(10517..12010)
                     /locus_tag="VFV99_29325"
     CDS             complement(10517..12010)
                     /locus_tag="VFV99_29325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063528.1"
                     /translation="MTALLRRLRRDVRAEVTEVPARMGTFCYSRRPRMWQEGQTDPAL
                     APGTMVGEYRVEGIVGEGGMGQVYGAIHPVIGKRAAIKVLLPEICRNQQMVERFVMEA
                     RAVNQIGHPNIVDVFAFGVLPDGRQYMVMEWLKGVSLSDRLKRGVPTIPETCDVLYAI
                     AAALDAAHAAGIIHRDLKPHNVFLVDVRGAKPLVKLLDFGLVKHNDDNDPRANKTQAG
                     ALLGTPAYMSPEQALGRSIDTRSDNYALGVLAFEMLTGRLPFIEDTAMALLVAHMQAP
                     PSLPSSVAPGIPGELDRLVLGLLAKDPNARPTLTQVMQVLAYYRDAQHPSQIPTHPRG
                     IATVIAPQAVPPPHALSTMGQSSGQMLTERHAPKKRTGLYALAALGVVGAAGAAIAVV
                     LSKKPADKPEPAAQPPVVATPADAATVAPPPTPDAATAVVAAPADAAVEVAATPDAAT
                     STVAVDTPKPTPPKKKPPKRPKPDPGTTPPPGGDDDDGLIRVTPKKN"
     gene            12044..>12290
                     /locus_tag="VFV99_29330"
     CDS             12044..>12290
                     /locus_tag="VFV99_29330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063529.1"
                     /translation="MTLTRVGLLVALAACGKDPATAGDDESTPDAGEITPTGYTRLIG
                     RTWELPAGANIYKCARVTVPADMYITTIIVKKPLGTHH"
BASE COUNT         2137 a         4005 c         4010 g         2138 t
ORIGIN      
        1 gaattgttcg cctgcatccc atgccgcagg ctggtcccgc acgacccgtt cggcgtgagc
       61 gcgacgcgcc tcataggtgt ctggatcgtt ccacggcggc gtgagcggcc aatcatacca
      121 gtgctgcacc gtcggatccg catcccattt catgaccgtc actacgtctg cgagctcgaa
      181 gggagccaga cagatgacac catcgcttac ccggttctcc ggcgcgtcga tggagatggc
      241 catgggttgc cagtttaggg gcgaaaacgc gaaaacggac cgccttgcgg gcgatccgtt
      301 tcacgcttcg aagcgactgc gcgttactcg acgcgctgga tgatgtgaat gccgaaatcg
      361 gtcttcacga cgcccgtctc gccaaccttc aggcgcagcg acaggttctt gaacggctcg
      421 acgaggcccg cgtcgggcgt cacgtcgtaa ccttcaccac tcttcgccga gcccggatcc
      481 tccgagagct ccgccatcag cggctcgatc ttcgagccct tctgcagctt ggcgaccgtg
      541 tccttgacga gcttctcgag ctgcggacgc gtgcgcttct tcgcgcgctc gtcgccgccc
      601 gcgttcactt cggaccagcc gaggaggatg tgcttcacct tggccttctg cgtttgcggc
      661 tcgcgcttga ggatgtctgc cgactccagc ggatccggcg gcggcggcgg gatgcgcttg
      721 atgatgtgcc agccaaacgg cgacttcacg agaccaactt cgccttcctt caggcggagc
      781 gagaggttct tgaacggctc gaccatcggc gcgtcggggc tgaccggata ggcacggccg
      841 ttgtccttgc tgcccggatc ctcgctgaag tctttcatca gcttcgtcat gtcctcgccc
      901 ttctgcgcgc gggccaggat gtcttgcgcc atcttgtcgg cttcggcctt cgtgcgatct
      961 ttcgcgcgcg gatcaccgcg gcccgacttc gcagccggcg cgtccttcca accgatgagg
     1021 acgtgctgga catggacggt gcccttctcg ggcggctcgc gcttcaggat gtccgctgac
     1081 tcgagcggat caggcggcgg cggcggcacg cgctccatca cgtggtagcc gaacttcgtc
     1141 ttgacgatgc cgacttcctt ctctttgagg tgaacggcga gtgccttgaa ctcgggaacg
     1201 aacggcgcgt ctgccttgac ctcgtacggt tcgccggaga gcgagcccgg atcctcggag
     1261 tgctccttga tgagtgcgtc gatcgagtcc ggatccttgc gcaacttgtc cgcgatctct
     1321 ttggcgagct tggcggcgtc ggcgttcgtg cgttttgcgg cgcgcggatc gagccggcct
     1381 tgatagacct cgtcgagatc cttccagccg atcaggacgt gcttgacctg aacgacgtcg
     1441 gtcgcttcct tgcggttgag gatgtccttc gagtcgatgt cgatcgcgtc gccgcccttc
     1501 gacgtcgtgg caccgctgcc ggtggtcgtc ttggcagcag gcatctcatc gagcttgctc
     1561 ttgccctggt cgcaggcggc tgcgaccacg agtaaaatcg cgaataccgt tttacgcata
     1621 cggcgcgcat cctagctcgc cggatccgtc tgcgctcgct tcatgttcga agatcggcct
     1681 aggcggggtt gtttccgaca gaggttcccg aggccccggt ccgtctccgg ctgggtatca
     1741 cacgatggat gagcggcgat ccgccggcac gaacaccagg aacaataccc acgcaccgac
     1801 gagaatcgcg gcgtagacga gcgcgagcgt cgggtagcgc acgatcatcg cgacgatgca
     1861 gagcagcgac agcgcgagtg cgatccacgg cgtgaacgga tagcccggcg tgcggtacgg
     1921 gcgcggcagc tcaggctcgc gcatgcgcag tcggagcacc gcgatcgacg acagcgagta
     1981 cagcgtcagc gcgccgaaca ccgcgatcaa gatgatgtcg ctcgtcttgc cggtcgcgag
     2041 cgcgatcatg ccgacgccga gattcacgag cagcgcgacc acaggtgtct tcgtgcgcgc
     2101 gttgatctcg ccgacgatcg ccggcgcgta gcgggcgcgg ccgaactcga ggatcgcgcg
     2161 actcgccgcg atcaagatgc cgtggaagct ggcgatgaga ccgatcaggc cgatgccggt
     2221 cagcacgaca aacagcggac tctcgcgcga cacgatgtga ccgatcgcca tcggcaacgg
     2281 cgagtcggtg gtcaccgcgt ggttcgccgg atcggggtag acgactgcct gccagcccgc
     2341 ggcaccgacg gcgccgaata gcgtgactgc agtcagcgcg acgagcgtgg ccatcgccca
     2401 caagaatccg cgcggcaaat cgcgctgcac gtccttcgct tcctccgcga cgttcgcgat
     2461 gccttcgatc gcgaggtaga accacatcgc aaacggcagc gctgcgaatg caccgcccca
     2521 gccgtgcggc agcgcgtcgg cggagaagtt gtcccacgag aagctcggca gcgcgacgga
     2581 gccgaacacg cacagctcga tcacggcgac gacggtgagc acgagctcga acgtcaccga
     2641 cagcttcatg ccccagatgt tgatcgcggt gaacacgaca tacgcagcga ccgcgatcac
     2701 cggaatcggg atcgatggaa tggtctggtt gatgtagctg ccgatcgcga acgcgatcgc
     2761 cggcggcgcg agcatgtact cgacgagctg cgcaacgccg ccgatgaagc cgaggtgcgg
     2821 cccgaatgca cgtgatgtgt aaacgaacac gccgcccgcc ttcggcagcg cgcaggccag
     2881 ctcggcatag ccgagcacga acgtcacgta gaggatggtg actactccag tggccaccaa
     2941 cattccgtac ggcccaccct cgggcagccc gaggttccag ccgaagtaca tgccggagat
     3001 gacatagccg acgccgagtc cccagatgag aaccggaccg agcgtcttct gcagctgcgt
     3061 catgacgcgt tagctccgtg cgattttcgc ggctgcgtgg tcatcggccg ttagctctcg
     3121 actgcaagtc aacagtgtat atgctccgcg ccgtccatga ctccacctgc cctgcctagc
     3181 cgcgcacgcg tcgttatcgt cggtggcggc atcatcggtt gctcggtcgc gtatcacctc
     3241 gcccacatgg gttggaagga tgtcgtgctc ctcgagcgtg accgattgac gtcgggaacg
     3301 acatggcacg cagcgggatt gatcgtcact ttcggatcac tgtcggagac gtcgaccgag
     3361 atgcgcaagt acacgcgcga tctctacgcg aagctcgaag ccgagaccgg ccaggacacc
     3421 ggcttcaagc cagttggctt catcgaggtc gctgccgaca aggatcgcct cgaagaatat
     3481 cggcgcgtct ccgcgttcaa tcggatctgc ggcgtcgacg ttcacgagat ttcgcccggc
     3541 caggttcgcg agctgtttcc gatcgcgaag accgacgaca tcctcgccgg cttctacgtc
     3601 aaagaagacg gccgcgtgaa tccggtcgac gcgaccatgg cgctcgcaaa gggcgcgcgc
     3661 atgcagggcg cgaagctgat cgaaaacgtg ccggtcaccg gcttcctcac gaagcgtggc
     3721 gccgtcaccg gcgtgcgcac cgcgtacggc gacatcgagg ccgagttcgt cgtcaactgc
     3781 gccggcatgt gggcgcgcca gatcgcgcgc gaggtcggcg tcaacattcc gctgcaggcg
     3841 gccgagcact actacctgat caccgagcgg atgaagcaaa tctcgccgga ctggccggtg
     3901 ctcgaggatc cgggctcgta tggttacttc cgcgaggaag tcggcggctt gatgatcggc
     3961 ctgttcgagc cgaagtgcgc gccgtggaac gtcggcggca ttccgggtga cttctcgttc
     4021 ggcgagatcc agccggactg ggagcgcatg ggcccgtacg tcgagaaggc gatggcacgc
     4081 gtaccgatct ccatggagac cggcgtgaag aaattcttct gcggtccgga aagcttcacg
     4141 cccgacctcg caccgatcgt cggcgaggcg cccgagctcg agaatttctt cgtctgcgcg
     4201 ggtctcaact cgatcggcat catcaccggc ggcggtatgg gccgcttgat ggcgcactgg
     4261 atcatcaacg gtcgccccga tcaggacatc agcgcgatcg acatcgcgcg cctgcatggc
     4321 taccaatcga atcccgagta ccgccggctg cgcaccgtcg agtcgctcgg catggtctac
     4381 cagtgccact acccgttccg gaatctcgag accgcacgcg gcgcgaagaa gtcaccgttc
     4441 tacgatcggc tcgcggcgcg cggtgcgtac ttccgcgacg tcagcggctg ggaaggcgcc
     4501 gactggtacg gctgcgatga caacacgctc acgttcaaga ggccgccgtg gttcccgcag
     4561 tggcaacgcg agcacactgc cgcacgcgaa ggcgtgatcg tgatggacat gtcgttcatg
     4621 tcgaagttcc tcgtcgaggg acgcgacgcc ggccgcctgc tcaactggat cagcgcgaac
     4681 aacgtcgatg gccagaccgg ccagatcacg tacacgcagt ggctcgacga agccggcaag
     4741 ctgcaaggcg acctcacggt cacgaagctc gcggacgaaa agtactgggt cgtcgccagc
     4801 gataccgtgc accgccacac gcagtggtgg atgaagcggc acttcggcga cgagcatgca
     4861 ttcgtcgccg acatgaccgc ggcatacgca cagctcaacg tgcagggacc gcgctcgcgc
     4921 gagctgatgc agctcgtgac gacgaccgac atgagcaacg atgcgttccc gttccgcgcc
     4981 gcgaaggaga tcgatatcgg cttcgcgcgc gtgctgtgca tccgcatcac gtacctcggc
     5041 gagctcggct acgagctgta catccccacc gagcaagcgc tgcacgtcta cgatcggctc
     5101 gtcgaggccg gcgaaaaggt cggcctcgtg cacgcgggcc tcaagacgct cgcgtcgtgt
     5161 cgcatggaga agggctaccg cgacatgggc cacgacatcg acaacaccga cacggtgctc
     5221 gaggcgggcc tcggcttcgc ggtcgatctg aagaagccag gcggcttcct cggcaaggac
     5281 gcgatcgtcg cgcagaaggc ggcaggtccg ctgaagaagc ggctgttgca gatcctgctc
     5341 aaggatccgg agccgctgat gttccatgcc gagatcgtga agcggaatgg caagccggtc
     5401 ggctacatcc gcgcggcgtc gtacggccac acgctcggcg gtgccgtcgg tctcgcgatg
     5461 atcgaagcgc ccgagccgat cgatcagaag tggatcgatg ccggcgcgtg ggaggtcgag
     5521 attgcaaacc agacctaccc cgcggtcgcg tcactgaagc cgctctacga tccggagaac
     5581 aagaaggtaa agctgtaagc tcggcgcgtg gtcgcacgtg ggccaggcga tgtcggatgc
     5641 gcgcactgca cgttgatcgg cgcggatcga ccgtgcccgg tctgcacgca cctcgtctgc
     5701 gagaggtgcg cgagcgactg gacaacgtgc agcgagccgt cgggtcgcgt catccggctc
     5761 ggcttgaccg cgcgtgtgcg cgacgtcgat ccgtcgggcc gactcgcact cgtctcgcac
     5821 tggcgccagc cgctgcgcgt gttcgacttg cgtcagctgc gctgggtccg agatatccgg
     5881 ttgcctcgcg gcgcatacct gtggaaccgc acccggccgc cacgccttac gagcgatgga
     5941 cgtttcatcc acgcggtatg gggtgcaggt accggctacg aacgaaccga cacgctcgtg
     6001 ttcaagggca tgcgattcga caagctgggc agcaataaca cggagctcgt gccctctgcc
     6061 gaggttcccg atcacggcac ggcggtgtcg ccgacgcgcg acgtcttcta ttacgtgacc
     6121 gacacgcagc gggtcgcgct gatcgacggc ggacgcgcgg tttccgtcga gccgttgccg
     6181 cgcaaggtcg tccactcggt gtacttcgat gcggaacgcg agctgcttgc gtgcggcggt
     6241 tggaaagaga tcgtcctcga tcacacggtc gatggtcacc tgagtcgcgt cacgcgtatc
     6301 gagaccagga acaccggcga cgtgcactgg ctcgcgctgg cgggcggctg gctcgtcgcc
     6361 gcgatctggg tcagcggcac gagcgcgatc gagatccgcg agctttctga caacctcgcg
     6421 atcggtccgg tccggcatcg ccacgtgacg acgcggctgc gtgcagcagc tctgtcgcgc
     6481 gacggccgtt acttcgcgtt tggcgactac aacggtctcg tcgtccacga gctcgcgacc
     6541 gatcgcacga cggtgttcac cgagcactcc gacgagatca atttcgtcag cttctcgcgc
     6601 gacgatcaca cgctgatctc ggccgacacc gacaatcgcg tcgtcatgcg cccgcggaca
     6661 gaggccggct acgcgaagcc ggtgatcgcg atcgatgtcc ccagcgaagg cgtcgatcta
     6721 ccccgcttcc agccccgcgc ggtcccctga aagccgagta gtccggcttg cagaaacgcg
     6781 tgcttgtcgc cgtttttcgt ggctggcgcg gagttagccg catctggcaa acgcgagccg
     6841 gcagcatctg gggtctcgcg gtgatgcagg ggtgtcgcaa gcatgtaatg agacacccgc
     6901 cgcagacagc gcacttttcg gtcgcacggc ggtctaaatc gacgatgatg cccaccatga
     6961 tgaggcggta cacgctggcg ttgctattgg ctttcggctg cggtggtacg aacccgatgg
     7021 gcggcgacga cgtccagccg ccgatggatg ggaacggcag caacggcggc gacgccgaca
     7081 cgttccagcc accggctggc tacacgaagc tgatcggccg cacgtggacg ctggcagcgg
     7141 gacagcacga catctacaag tgcgtgcgca tccacgtccc gacggacacg tacatcacga
     7201 gcatcatcgc gcaggcgcct cccggcacgc accacaccgt gctctcgatc gcgagcagct
     7261 tgggcgggtc ggctggccca gatggcgagt atgactgcga agtgcggacg ctcggcctca
     7321 acatgctgta cgcctccggc gtcggcacgt cgccgctcga cttccccacg gacgtcggcg
     7381 tcaagatcgc cgcgggcacg cagatccacc tgaacctcca cctctacaac gcatccgacg
     7441 acacgctcag tggcgactct ggaatctggg tgaagtcgca gccgacggcg ccgccgatgc
     7501 tcgccgagat gcagttcgct ggtccgctcg acttgaccat tccaaagatg tcgaccaact
     7561 atccgatcac gagtacgtgc acgatcaaca actcgtacca gctgttcgca ctgtggccac
     7621 acatgcacaa acatgccgtt caccagaaga tcgagctgct ccgtggcacg gcgcaaccgg
     7681 tcacgctgca cgacatgccg tatcagttcg gcgagcagaa gtactggccg cagagcccgg
     7741 aaatccaact gcagcctcag gacaagatcc gcgtgacatg cagctacgac aaccctacga
     7801 atgccgacat caactggggt gatagctccg acgaagaaat gtgtttcggc ggcatgtacc
     7861 gctatccgtc ccagggcagc atcttgttct gccagaactg atcgcacgag cgtgatctgg
     7921 agcgtggcgc gcggacgcgt caccggggta tcgtcgctaa cgatgcgacg tacaactcgt
     7981 tgggtgctgg gcggcgtcct ggcagcggtg gcgctcgtta gcgcgagcgg ctgcttcaaa
     8041 ggcgaattcg caaactgcaa agtgacctgc gcgaccagcg ccgactgccc cggcagcctc
     8101 gagtgcaatc cgcagggcgt ctgctcgcca ggcggcgaca tgtgctcggc aatgcccgac
     8161 gcggatctgt cgaagccgca gacgttgacg gtgtcgacga tgggggacgg cgcggtgacc
     8221 tccgaccccg ccggcatcga ttgcggcgcg acctgcatgc acgacttccc gccgctctcg
     8281 tcggtgacgc tcactgcggc gccggcgggc acgtcggtgt tcgacggctg gaccggcgac
     8341 tgcgcgggcc aggccgagac gtgctcgctg acaatggacg ggccgaagtc ggcgaccgcg
     8401 aagttcgcga cgcacggcgc gctgcgcttc gtgaagcaga tcagcttctc cggtcaggat
     8461 ttcgtcggta gcgagctcgc ggtcgattcc aacggcgacg tgatcgccac cggctcgatc
     8521 gacgatcagg gcgcgcccgg cttctacacc gtgaagtacg gcaaagacga cggtcacatc
     8581 atctgggaga agaagacgat cgtctcgggc ggcttcttcg gcatcggtgg tctcacgacc
     8641 gatagcagtg gcaacgcgta catgtgcacg cggttccagg gccagggcgc ggcgacgatc
     8701 ggcccgtcga ccgtcacggg tgacctgttt ggcaacgtgc tcgtcgtgaa gtacgccgct
     8761 gccgacggca acatcgagtg ggcgaagtca tggggcggct cggcccagga agagtgcgcc
     8821 ggcatcgtgg cgtcgggcac ggacatcttc gtgaccggct acacgtcgag caatccgtcg
     8881 aacttcgaca gccaggtgct cgcgggctcg gtcaacaacg gcttcgtcgt gcgcgcggtg
     8941 cagtcgaacg gccaggctca acaggcgatc ctgctcggcg cgaacttcca gatctacgac
     9001 attgccgaga accaaggaca gatcgcggtc gtcggttcgt tcacggctag caactacatc
     9061 accggcagct gcaacttcac gtcgacggcg accggcaccg acgggttcat catgaacatg
     9121 tcgagcaacc tcgtgtgcac gtggaacaag acgttcggca gcctgaccgc gaaccaaaac
     9181 acggcgagct ttgcagtcgc cgatgtaccg ggaggcggct gggctgttac tggctacttc
     9241 gagggctcgg tgaacttcgc cggcaccggt tcggccgtcg gtaaccaagg cggccgtgat
     9301 gcgttcatcg cgcgctatgc gggaaacggc acccacatct attcgttcgg ctacggcgcc
     9361 gccggcaacg actatgggcg cggcgtcgac gtcacaccga ccggcgaggt ggtgttcggt
     9421 ggcgagtttc agaacacggt caccttcggc gcgaccatgc tcacgacgac aaaccaggac
     9481 gtcttcatga cgcgcctgtc cgcgggcaat acgccgaccc atcagtgggc ggccaaggtc
     9541 ggcggcacgg cgagcgaaca ggtgcagggg ctcaaggtcg atgcgcaggg cacggtgagc
     9601 gtgctcgcct actggaccgg catgaccgac atcggtggca tgcagctcac ggctgctgac
     9661 tacgacgcgt tcgtcggctc gttcgtgcgc tgagctgatc gttcagaagc gccaggcgta
     9721 gccggccgca gcgccgccgc gcgtcagctg cagcgacact tccggctgct gcgtcgcgcc
     9781 gctgcgcgat agcacgagga acacgacgcc ggtcgcgagg aacgcgccgc cggcgacgct
     9841 cgcgatcacc gcgttgcggt ttgccgcgtt gccgtcactc tcgagcgcct tcgcgtcagc
     9901 gccggtgcac gacgtctcgc acagcttcgc gagatcgtcg ttcgccgatt tgccgtcgag
     9961 cgcgaacttg atcgcgagac cgccgccgag cgcgccaacg ccaatcgccg cgatgcccgc
    10021 ccacttcatc cagccgcgcg ggttcgccgc cggcggcgtc tcgggccgtt cctcgtcttc
    10081 cggcttctcg agcggctcgg gctgtgccgt tgttgtcgtc ggcgtcgtcg tggtcggcgt
    10141 tgtcgtggtc ggcgtcgtcg tcaccggcgg tggtgtggcc ggctgggtct cggcgcactt
    10201 ttccatctcg gtgatgcgct cgcggacctt cgccgcgttc ggcgccttcg gcacgcggcg
    10261 cagatacgtc ttgtagaacg tcgccgcctc gcggcagtcg cgcttgaggc gatacgcctg
    10321 cgcgatgttg tagaggtagc ccggctcgtc agagatccgg aacgcttctt tgaagtcgga
    10381 aatcgccgcg tcgtactccg cgagctcgaa gtgcttgttg ccgctcgtct cgagcgccga
    10441 cacgtctggt ttctgctgcg ccgacgcgac cgccgtccac agcatgacaa aggtgattgc
    10501 aacgaagcgc ttcatgtcag ttcttctttg gtgtcacgcg aatgagccca tcatcgtcgt
    10561 ctccgccagg cggaggcgtc gtgccgggat cgggtttggg cctcttcggc ggcttcttct
    10621 tcggcggcgt tggcttcggc gtgtcgaccg cgaccgtcga cgttgcagca tcgggcgtcg
    10681 ccgcaacttc gaccgctgca tcggccggcg cggcaacaac cgccgtcgca gcatcgggcg
    10741 ttggcggcgg cgcgaccgtc gcggcatcgg caggcgtcgc gaccacgggc ggctgcgcgg
    10801 ccggttctgg cttgtccgcg ggcttcttgc tcagcacgac cgcgatcgcc gcgcccgcag
    10861 cgccgacgac gccgagtgct gcgagcgcgt agagccccgt gcgcttcttc ggcgcatggc
    10921 gctcggtcag catctggccg ctcgactgcc ccatcgtcga cagcgcgtgc ggcggtggca
    10981 ccgcttgcgg cgcgataacg gtcgcgatgc cacgcgggtg cgtcgggatc tgcgacggat
    11041 gctgcgcgtc gcggtagtac gcgagcacct gcatgacctg cgtgagcgtc ggccgtgcgt
    11101 tcggatcctt cgcgagcagc ccgaggacca atcgatcgag ctcgcccgga atccccggcg
    11161 cgaccgacga cggcagcgac ggcggcgctt gcatatgcgc gacgagcagc gccatcgcgg
    11221 tgtcctcgat gaacggcagc cgaccggtca gcatctcgaa cgcgagtacg ccgagcgcgt
    11281 agttgtcgct acgcgtatcg atcgagcgac cgagcgcctg ctccggtgac atgtacgcgg
    11341 gcgtgccgag caacgcgccg gcctgcgtct tgttcgctcg gggatcgttg tcgtcgttgt
    11401 gcttgacgag gccgaagtcg aggagcttga cgagaggctt tgcgccgcgc acgtcgacga
    11461 ggaacacgtt gtgcggcttg aggtcgcgat gaatgatgcc cgctgcgtgc gcggcgtcga
    11521 gcgcggccgc gatcgcgtag aggacgtcgc aggtctcggg aatcgtcggc acgccacgct
    11581 tgagccgatc cgagaggctc acgcccttga gccactccat caccatgtac tggcgaccgt
    11641 cgggcaacac gccgaacgcg aacacgtcga cgatgttcgg gtgaccgatc tgattcactg
    11701 cgcgcgcttc catcacgaag cgctcgacca tctgctggtt gcggcagatc tcgggaagca
    11761 ggaccttgat cgcggcgcgc ttgccgatca ccggatggat cgcgccgtag acctggccca
    11821 tcccgccttc accgacgatg ccttcgaccc ggtactcgcc gaccatcgtt ccgggcgcaa
    11881 gcgccggatc ggtttgccct tcctgccaca tcctgggccg tcgagagtag caaaacgtgc
    11941 ccatgcgtgc aggtacttcc gtgacctcgg cgcgcacgtc gcgacgtaac cgccgcagaa
    12001 gcgcggtcac gacgggaacg cgtcttgctc gaatgcctga gcgatgaccc tcacacgtgt
    12061 cggcttgctg gttgcgctcg cagcatgtgg caaggacccg gcgacggccg gcgacgatga
    12121 atcaacgccg gatgcgggtg agatcacacc gaccggctac acgcgtttga tcggccgcac
    12181 gtgggagcta ccggctggcg cgaacatcta caagtgcgcc cgcgtgaccg tgcctgcgga
    12241 catgtacatc acgacgatca tcgtgaagaa accgctcggc acgcatcaca
//
LOCUS       DASUFX010000724         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_950504,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000724 DASUFX010000000
VERSION     DASUFX010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950504"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFV99_29335"
     CDS             complement(<1..215)
                     /locus_tag="VFV99_29335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5063530.1"
                     /translation="MSADRAFDDNSAVWLLGAPADTPAPPLARDLDVDVAIIGGGFTG
                     VATAYRLSHRFPQLGIALLEAKQLANGA"
     gene            complement(212..1645)
                     /locus_tag="VFV99_29340"
     CDS             complement(212..1645)
                     /locus_tag="VFV99_29340"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5063531.1"
                     /translation="MTGREQFIDGAWVDAIGGGRRDLIDPATEDVITNLPYGGADDAH
                     RAIDAAHRALPDWRARTSYDRGEILVRAAELMRQRAQLYGEVTVREAGKPLAEAMGEW
                     KVAAALFEYSAEEGKRVTGYQIPARVPTKRMMVLKEPIGVVGVITAWNFPAYNPARAI
                     AAALAAGCTVVCRPAELTPLSLMTVAATLEEAGLPAGVLNVVHGEPHPIGQAMLDRPE
                     LKKLSFTGSVAVGKLLMDGASRTMTRLSLELGGNAPVLVLPDVDVEAVARGAVGARFR
                     NAGQVCISPQRFFVARDQLKTFEEVVVEETRKLPVGSGLDPKTRIGPLISGRHRDRVE
                     GLIANATEQGATLRTGGKRPAGRGYFLEPAVLGNVAPTMPAFAEEVFGPLFAMTSFDQ
                     LDDAITAANSTRYGLAAYVFTNDLGAAMKAYERLDFGMVGINEWAPQAAEAPFSGRKD
                     SGIGHESGREGVYDNLETKLVSFGNVK"
     gene            complement(1642..2985)
                     /locus_tag="VFV99_29345"
     CDS             complement(1642..2985)
                     /locus_tag="VFV99_29345"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5063532.1"
                     /translation="MTAIQRVTEIPGPKSREMLARRERAVTKGLAKSTDVVIDRAEGA
                     RVVDVDGNVLIDLAGGIGMTLAGHCPPPVVAAVEAQARRLLHPCALVATYEPYVALCE
                     LLNEVTPGDFAKKTLLANSGAEAVENAVKIARAHTKRAGVLCFEGGYHGRTLLTLSLT
                     SKYGLFKQGFGPFAPEIYRIAAPEPYRRPRGMSEEAYVDMCITQLERALIAQIDPAAL
                     AAIIIEPVQGEAGFIPMPPRYLRRIRELCTQHGIVMIADEVQTGFGRTGTLFAIEQAG
                     VVPDVITMAKALGAGLPISATTGRADIMDAPHLGGVGGTYGGSPIACAGAIQAVQMIR
                     QPEFLAHARAIGDLLRTELTAMQARSKLIGDVRGLGPMMLIELVTDHEAKTPAAPNAL
                     AVIKRSVANGVLLIRAGLYSNCIRFMPPLTISEAELREALAVVGEALAYVEEHGP"
     gene            complement(2982..3602)
                     /locus_tag="VFV99_29350"
     CDS             complement(2982..3602)
                     /locus_tag="VFV99_29350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063533.1"
                     /translation="MRHTGPEHALQLEELQRIVFPTLADEQRFKAPHYRKHVELFPQG
                     QLVVVDGARVVGMTSTIRYDFDFAHPDHTFADIIQGGWLTSHQPDGKWLYGADIGTHP
                     EYRRRGVARALYATRQVLVRELGLSGQMTVGMPIGYGPLKQTMTAQAYYDELVAGRRD
                     DPTISAQRKIGFELRGLIPGYIDDPACDHYGVVLVLPAAKEVSFPS"
     gene            complement(3632..>4260)
                     /locus_tag="VFV99_29355"
     CDS             complement(3632..>4260)
                     /locus_tag="VFV99_29355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5063534.1"
                     /translation="NIIGGSHLAVEREKLYNIASLFRRDGSVEKQYKLHISPSEAKWW
                     GVSAGDEVRVFDTDRGKIAILIGEDVAFPELARIAAGKGAMLLFVPFNMDIRSGYMRI
                     RLCAAARCIENGMYAVLAGPIGNLPFVQGADIHYGEACVLTPCDLPFARDGVSDEATP
                     NVETMLLNELDIEVLRRSRLTGSLRTWLDRRTDLYAVRYTEGGKRSEV"
BASE COUNT          686 a         1495 c         1375 g          704 t
ORIGIN      
        1 gcgccgttcg cgagctgctt cgcttcgagc agcgcgatgc cgagctgggg aaaccggtgg
       61 ctcaaccgat atgccgtcgc cacaccggtg aagccaccgc cgatgattgc gacatcgaca
      121 tcgagatcgc gagccagcgg cggtgccggc gtgtcggcgg gcgcgccgag cagccagacg
      181 gcagagttgt cgtcgaacgc ccggtccgcg ctcacttcac gttgccgaag ctgaccagct
      241 tggtctcgag gttgtcgtag acgccttcgc ggccggactc gtgaccgatg ccgctgtcct
      301 tgcggccgga gaatggcgcc tcggcggcct gcggtgccca ctcgttgatg ccgaccatgc
      361 caaagtcgag acgctcgtat gccttcatcg cggcaccgag gtcgttcgtg aacacgtacg
      421 ccgcgaggcc gtagcgcgtc gagttcgccg cggtgatcgc gtcgtcgagt tgatcgaacg
      481 aggtcatcgc gaacagcgga ccgaacactt cttcggcgaa cgccggcatc gtcggcgcga
      541 cgttgccgag cacggccggc tcgaggaagt aaccgcgacc ggccggccgc ttgccgccgg
      601 tgcgcagcgt ggcgccctgc tccgtcgcat tcgcgatcag gccttcgacg cggtcgcggt
      661 ggcggccgct gatcagcggg ccgatccgcg tcttggggtc gagtccggag ccgaccggca
      721 acttgcgcgt ctcctcgacg acgacttctt cgaacgtctt cagctgatcg cgcgcgacga
      781 agaaccgctg cggcgagatg caaacctggc ccgcgttgcg gaaacgcgcg ccgaccgcgc
      841 cacgcgcgac cgcctcgacg tcgacatccg gtagtacaag cacgggcgca ttgccgccaa
      901 gctcgagcga gaggcgcgtc atcgtgcgcg atgcgccgtc catcagcagc ttgccgacgg
      961 ccaccgaacc cgtgaagctg agcttcttca gctccggccg atcgagcatc gcctggccga
     1021 tcggatgcgg ctcgccgtgc acgacgttga gcacaccggc tgggagcccc gcctcttcga
     1081 gggtcgctgc gaccgtcatc agcgacagcg gcgtcagctc ggccggacgg cacacaaccg
     1141 tgcagccggc ggcgagcgcc gctgcaatcg cgcgcgcggg gttgtaggcg ggaaagttcc
     1201 acgccgtgat cacaccgacg acgccgatcg gctccttgag caccatcatc cgcttcgtcg
     1261 gcacgcgcgc cgggatttgg tagccggtga cgcgcttgcc ctcctcggcg gagtactcga
     1321 acaatgcggc cgcaaccttc cattcgccca tggcctcggc gagcggcttg cccgcctcgc
     1381 gcaccgtgac ctcgccatac aactgcgcgc gttgccgcat gagctcggcc gcacggacga
     1441 gaatctcgcc gcgatcgtag gaggtgcgtg cgcgccagtc cggaagcgcc cggtgcgcgg
     1501 cgtcgatcgc gcggtgggcg tcgtcggcgc cgccgtacgg caggttcgtg atgacgtcct
     1561 cggtcgccgg gtcgatcaga tcacggcgcc cgccgccgat cgcgtctacc cacgcgccat
     1621 cgatgaattg ttctctgccc gtcatggtcc gtgctcctcg acgtatgcga gcgcttcgcc
     1681 aacgacggcg agcgcctcgc gcaactcggc ctcgctgatc gtgagcggtg gcatgaagcg
     1741 gatgcagttc gagtacaagc cggcgcggat cagcagcacg ccgttcgcga ccgagcgctt
     1801 gatcaccgcg agcgcattcg gcgccgccgg tgtcttcgcc tcgtgatcgg tgacgagctc
     1861 gatcagcatc atcggtccga gcccacgtac gtcgccgatc agcttcgagc gcgcttgcat
     1921 cgcggtcagc tccgtccgca gcaggtcgcc gatcgcacgc gcgtgcgcca gaaactcggg
     1981 ctgccggatc atctgcaccg cttggatcgc gcccgcacac gcgatcgggc tgccgccgta
     2041 cgtgccgccg acgccgccga ggtgcggcgc gtccatgatg tcggcacggc cagtcgtcgc
     2101 cgaaatcggc aacccagcac cgagtgcctt cgccatcgtg atgacgtcgg gcaccacacc
     2161 tgcctgttcg atcgcgaaca acgtgccggt gcggccgaat ccggtctgca cctcgtcggc
     2221 aatcatcacg atgccatgct gcgtgcacag ctcgcggatc cgccgcagat agcgtggcgg
     2281 catcggaatg aagcccgcct cgccttgcac cggctcgatg atgatcgccg cgagcgccgc
     2341 cggatcgatc tgtgcgatca gcgcccgctc gagctgcgtg atgcacatgt cgacgtacgc
     2401 ctcttccgac atgccgcgcg gccgccgata cggctccggt gccgcaattc gataaatctc
     2461 cggcgcgaac ggaccgaagc cctgcttgaa cagaccgtac ttgctggtca ggctgagcgt
     2521 cagcagcgtg cggccgtgat agccgccctc gaagcacagc acgccggcgc gcttcgtatg
     2581 cgcccgcgcg atcttcaccg cgttctcgac ggcttcggcg ccgctgttcg cgagcaatgt
     2641 cttcttcgcg aagtcgcccg gcgtgacctc gttcaacagc tcgcacagcg cgacatacgg
     2701 ctcgtacgtc gcgaccagcg cgcacggatg caacagccgg cgcgcctgcg cttcgacggc
     2761 cgccacgacc ggcggcggac aatgacccgc gagcgtcatc ccgatgccgc cggcgagatc
     2821 gatgagcacg ttgccgtcga catcgacaac gcgcgcgccc tctgcgcgat cgatcacgac
     2881 atcggtcgac ttcgcgagcc ctttggtcac cgcgcgttcg cggcgcgcca gcatctcgcg
     2941 gctcttcgga cctggaatct cggtgactcg ttggatggct gtcatgatgg aaagctcacc
     3001 tctttcgctg cgggcaacac gagcacgacg ccgtagtggt cgcacgctgg gtcatcgata
     3061 tagcctggga tcaagccgcg cagctcgaac ccgatcttgc gctgcgcgct gatcgtcgga
     3121 tcgtcgcggc ggccggcgac aagctcgtcg taatacgcct gcgccgtcat cgtctgcttg
     3181 agtggcccgt agccgatcgg catgccgacc gtcatctgac cggataggcc gagctcgcgc
     3241 acgagcacct gccgcgtcgc atacaacgcg cgcgcgacgc cgcgacgccg atactcggga
     3301 tgcgtgccga tatcggcgcc atacagccat ttcccgtcgg gctggtgcga cgtcagccaa
     3361 ccgccttgga tgatgtcggc gaacgtgtga tcgggatgcg cgaagtcgaa gtcgtagcgg
     3421 atcgtcgacg tcatgccgac cacgcgcgcc ccatccacca cgacgagctg cccctgcgga
     3481 aacagctcga cgtgcttccg gtagtgcggc gccttgaacc gctgctcgtc ggcgagcgtc
     3541 ggaaacacga tgcgctgcag ttcctcgagc tgcagggcgt gctcggggcc ggtgtgcctc
     3601 acgacgaggc catgcccgaa ccgccgctcg ctcacacttc gctccgctta ccgccctcgg
     3661 tgtagcgcac cgcgtacaga tcggtccgcc gatcgagcca cgtgcgcaag ctgccggtca
     3721 gacgactacg ccgcagcact tcgatgtcga gctcgttgag caacatcgtc tcgacgttcg
     3781 gcgtcgcttc gtccgacacg ccgtcgcgcg cgaacggaag gtcacacggc gtcagcacgc
     3841 acgcttcgcc gtagtgaatg tcggcgcctt gcacgaacgg caggttgccg ataggcccgg
     3901 cgagcaccgc gtacatgccg ttctcgatgc accgcgcggc cgcacacagc cgaatccgca
     3961 tgtacccgga gcggatgtcc atgttgaacg ggacgaacag cagcatcgcg cctttgccgg
     4021 cggcgatgcg cgcgagctcg ggaaacgcga catcctcgcc gatcaagatc gcgatcttgc
     4081 cgcgatcggt gtcgaacacg cgcacctcgt cgccggccga gacgccccac cacttggcct
     4141 ccgacggcga gatgtggagc ttgtactgct tctcgaccga accgtcgcgc cggaacagcg
     4201 aggcgatgtt gtagagcttc tcccgctcga ccgcgaggtg gctgccgccg atgatgttga
//
LOCUS       DASUFX010000725         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_810742,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000725 DASUFX010000000
VERSION     DASUFX010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810742"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1304)
                     /locus_tag="VFV99_29360"
     CDS             complement(120..1304)
                     /locus_tag="VFV99_29360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063535.1"
                     /translation="MAALRREFDGRPTLLIAAVVGIVGAIGVAIGIAIEPERAFAAYL
                     AAWCAVATVAIGGLALLVIGYAANSRWPAAVRRLTEAIAGGLLPVAILAIPLFLGAEW
                     VWPWVAPDGRYLHAVEVKQTYLSTPFFVFRTELYFVAFIIPAELLIRWSRRRDARPEP
                     PAPLRTDALHRERVLSSAVLPIVGLAITFAAFDWLMSLQPAWWSSGFGLYVLTGALTA
                     GLAMVAVLAWRGVAERCMPLTPGHFHAMGRLLHAFVILWAYIAYFQVMLIQIANKPNE
                     AQFYVDRGTDGWRFVTALLVVLGFALPFPLLFPRRLKRRAGYVGGIAVMLLVGHYLDM
                     WWLVIPRVSAMPIPSWTDLAALCAIGGLVTAVCTWRMRGVPLLPVGDPYLATGLAYET
                     TT"
     gene            complement(1292..1810)
                     /locus_tag="VFV99_29365"
     CDS             complement(1292..1810)
                     /locus_tag="VFV99_29365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5063536.1"
                     /translation="MTRALLALLAIAGCDWSLHRMQAPDKCTVHGTTTLLPNDSCNLE
                     PPAGIVAMDTPAPPPPITRALLERGHDRFDRMCAACHGLRADGDSPVARAMTLRRPPS
                     LIDPRVAAFTDERILTVITTGYGLMPEYATSLAPPDRYAVLHYLRALQQRDVALDQLS
                     PSLQAEARRWLH"
     gene            complement(1807..2337)
                     /locus_tag="VFV99_29370"
     CDS             complement(1807..2337)
                     /locus_tag="VFV99_29370"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX5063537.1"
                     /translation="MRRGIAAEYLTKESLLAAIRAMRARGLVQLEAYTPVPFREVDAA
                     LGARRSPLAIAAGIGGLAGAVGAYFLQWLLVAYLYPIDLGSRPPHMPLPFTIITVEMG
                     FLVGALTVFVAFVIVSRLFKLWDPIFDVPGFESATRAGFWLAVGADDPAWDRDAITHA
                     LQAIRPTQLHGFGGIG"
     gene            complement(2334..3626)
                     /gene="nrfD"
                     /locus_tag="VFV99_29375"
     CDS             complement(2334..3626)
                     /gene="nrfD"
                     /locus_tag="VFV99_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5063538.1"
                     /translation="MTELERVHDDLFTRVRARPGLHWWSAFALCGAGTGMLGVLIAYT
                     VATGIGVWGTNIPTGWAFAIINFVFWIGIGHAGTFISAILYLLEQNHWRNPISRLTET
                     MTVFAVVQAALFPILHLGRPWFAYWLLPYPDVMGVWPQVKSALPWDAAAVTTYLTVSV
                     VFWYVGMIPDLASLRDHAPSLRARKLYGLLAIGWRGDSRSWRHWKVTYLMLASIATPL
                     VVSVHSVVSSDFAIGLTPGWHSTLQPPYFVGGAIFSGFAMVLVLAIPIRVLYGIEHVI
                     TDRILDNCAKLMLVTGWIVTYGYIVEHFGAYYSGEPAERSLMFGHHPGSLAFWTMLSC
                     NAIIPQLLWWRRVRRAKLVLFVISVLVLVGLWFERYVIIVQGLMRDQLPSAWGSYTPT
                     WVDLGILVGTFGFFGLLFLVFLRLMPFVPVSEIKEEVE"
     gene            complement(3623..>4714)
                     /locus_tag="VFV99_29380"
     CDS             complement(3623..>4714)
                     /locus_tag="VFV99_29380"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5063539.1"
                     /translation="DRLGVADGSRLELTTTAGQLALPVIVVPGAADGSITVELGYGQH
                     VPQLPIADQIGGDGYALRTDDRLLLHGAVRVLAGKTVLPRTQMTFAEHGREIAPAATL
                     AAFAAQPNFVAHLRGEQASLLPPQPRDGTQWGMSIDTSICTGCSACMVACQAENNIAT
                     VGPRDVARGRHMNWIRIDRYVREDGVVVNEPMPCQHCEYAPCEYACPVNATTHSPDGL
                     NEQVYNRCIGTRFCSNNCPYKVRRFNWFAYEQTSTRALQYNPDVTVRSRGVMEKCTYC
                     VQRIRRAEHRAIVEHREVRAGEVVTACQQACPTGAILFGELHERGTAFAATRTDARSF
                     NALHELGTRPRTQYLAKIRNPRDQTRGRK"
BASE COUNT          875 a         1528 c         1642 g          669 t
ORIGIN      
        1 aaacgccgcg agtgaccaga cgacgacggc acacgtcgcg atcacgagcg cggtgatcgc
       61 gagcgtcaac ccgatcgcgc gaccggggac attctcgggc tgttctggga tctcgacagt
      121 catgtcgttg tctcgtaggc aaggcccgtg gcgaggtacg gatcgccgac cggcaggaga
      181 ggaacgccgc gcatgcgcca cgtgcacacc gcggtcacga gcccgccgat cgcgcacagc
      241 gccgcgaggt cggtccacga cgggatcggc atcgcgctga cgcgcgggat cacgagccac
      301 cacatgtcga ggtaatgccc gacgagcagc atcacggcga tacctccgac gtaaccggcg
      361 cgtcgcttga gccggcgcgg gaacagcagc gggaacggca acgcgaagcc gaggacgacg
      421 agaagcgccg tcacgaatcg ccagccgtcg gtgccgcggt cgacatagaa ctgcgcctcg
      481 ttcggcttgt tcgcgatctg gatcaacatc acctggaagt aggcgatgta cgcccacagg
      541 atcacgaacg cgtgaagcag ccgacccatc gcgtggaagt ggcccggggt gagtggcatg
      601 cagcgctccg cgacgccgcg ccacgcgagc acagcgacca tcgcgagccc cgccgtcagc
      661 gcgcccgtga gcacgtagag accgaatccc gacgaccacc acgccggctg cagcgacatc
      721 agccagtcga atgcggcgaa cgtgatcgcg agaccgacga tcggcagcac cgcggatgac
      781 agcacgcgct cgcggtgaag cgcgtcagtg cggagcgggg cgggcggttc cgggcgcgcg
      841 tcgcgccgtc gcgaccagcg aatcagcagc tcggcgggga tgatgaacgc aacgaagtac
      901 aactcggtgc ggaacacgaa gaatggggtg gataggtacg tttgcttcac ctcgacggcg
      961 tgcaggtagc ggccatcggg cgctacccac ggccacaccc actcggcgcc gaggaacaat
     1021 gggatcgcga ggatcgcgac ggggaggagg ccaccggcaa tcgcctcggt aagcctgcgc
     1081 actgccgccg gccaccgcga gtttgccgcg tagccgatca cgagtagcgc gagaccgccg
     1141 atcgcgacgg tggcgaccgc gcaccacgcc gcaagatacg cggcaaacgc gcgctcgggc
     1201 tcgatcgcga tcccgatcgc gacgccgatc gcaccgacga tgccgaccac cgctgcgatc
     1261 agcaacgtcg gccgcccgtc gaattcgcgc ctcagtgcag ccatcgccgc gcctccgctt
     1321 gcagagaagg tgagagctgg tcgagcgcaa cgtcgcgctg ctgcagcgct cgcaggtagt
     1381 gcagcaccgc gtagcgatcg ggtggcgcaa gcgatgtggc gtactcgggc atcagcccgt
     1441 agccagtcgt gatcacagtg aggatgcgct cgtcggtgaa cgctgcgacc cgcggatcga
     1501 tcagcgatgg cggccggcgc agcgtcatcg cgcgtgcgac gggcgagtcg ccgtcggcac
     1561 ggaggccgtg gcaggcggcg cacatgcggt cgaagcgatc gtgcccgcgc tcgagcagcg
     1621 cgcgcgtgat tggcggtggt ggcgccggtg tgtccatcgc gacgataccg gcaggcggct
     1681 cgaggttgca gctgtcgttc ggcagcagcg tggtcgtgcc gtgcacggtg cacttgtcgg
     1741 gcgcttgcat gcggtgcagc gaccagtcgc agcccgcgat cgcgagtaga gcgagcaacg
     1801 cgcgcgtcat ccgatgcccc cgaagccgtg gagttgtgtg ggccggatcg cctgcagcgc
     1861 gtgcgtgatc gcgtcgcgat cccacgcggg atcgtcggca ccgacggcaa gccagaaccc
     1921 ggcgcgcgtc gctgactcga agcccgggac gtcgaagatc ggatcccaca gtttgaacag
     1981 ccgcgacacg atgacgaacg cgacgaacac tgtcagtgcg ccgacgagaa agcccatctc
     2041 gaccgtgatg atcgtgaacg gcagcggcat gtgcggcggc cgcgagccga ggtcgatcgg
     2101 atagaggtag gcgacgagga gccactgcag gaaatacgcg ccgacggctc cggcaagtcc
     2161 accaatgccg gccgcgatcg cgagcgggga ccgcctcgct ccgagtgcgg catcgacttc
     2221 gcgaaatggc accggcgtgt acgcctcgag ctgtacgagc ccccgcgcgc gcatcgctcg
     2281 aatcgcggcg agtagcgatt ccttcgtcag atactcggca gcaattccgc gcctcactcg
     2341 acctcctcct tgatctcgct gacgggaacg aacggcatca accgcaagaa cacgaggaac
     2401 aagaggccga agaagccgaa cgtgccgacc aagatgccga gatcgaccca cgtcggtgtg
     2461 taacttcccc acgcagaggg cagctgatcg cgcatcagcc cttgcacgat gatcacgtag
     2521 cgctcgaacc acaagccgac gagcacgagc accgagatga cgaacagcac cagcttggcg
     2581 cggcgcacgc ggcgccacca caacagctgc gggatgatcg cgttgcagct gagcatcgtc
     2641 cagaacgcga ggctgccggg atggtggccg aacatcagcg agcgctcggc gggctcgccg
     2701 ctgtagtacg cgccgaagtg ctcgacgatg tagccgtagg tgacgatcca accggtgacg
     2761 agcatcagct tcgcgcagtt gtcgaggatg cgatccgtga tcacgtgctc gatgccgtag
     2821 agcacccgga tcgggatcgc gagcacgagc accatcgcga agccggagaa gatcgcgccg
     2881 ccgacgaagt agggcggttg cagcgtcgag tgccagccgg gcgtgaggcc gatcgcgaag
     2941 tcggatgaca cgacgctgtg caccgagacc acgagcggtg tcgcgatgga tgcgagcatc
     3001 aggtacgtca ccttccagtg gcgccacgag cgtgaatcgc cgcgccagcc gatcgcgagc
     3061 agaccgtaca gcttgcgtgc gcgcagcgac ggcgcgtgat cgcgcagcga cgccagatcc
     3121 ggaatcatgc cgacgtacca gaagacgacc gagacggtga gataggtcgt gactgcggcg
     3181 gcgtcccatg gcaacgccga tttgacctgc ggccacacac ccatcacgtc ggggtagggc
     3241 aggagccagt acgcgaacca tggccggccg aggtggagaa tcgggaacag cgccgcctgg
     3301 acgaccgcga acacggtcat tgtctcggtg agccggctga tcgggtttcg ccagtggttc
     3361 tgctcgagca gatagaggat ggcggagatg aacgtgccgg cgtgaccgat tccgatccag
     3421 aagacgaagt tgatgatcgc gaacgcccac ccggtcggga tgttcgtacc ccagacgccg
     3481 atacccgtcg cgacggtgta ggcgatcagt acgccgagca ttccggtccc cgcgccacac
     3541 agcgcgaacg ccgaccacca gtgcaggccg ggccgcgcgc gaacgcgggt gaacagatcg
     3601 tcgtggacgc gctcgagctc ggtcatttgc gccctcgtgt ctggtcacgt ggattccgga
     3661 tcttcgccag gtactgcgtg cgcggccgcg tcccgagctc gtgcagtgca ttgaagctgc
     3721 gtgcgtcggt gcgcgtggcg gcgaacgcgg tgccgcgctc gtggagctcg ccgaacagga
     3781 tcgcaccggt cgggcacgct tgctggcatg cggtcacgac ctcgccggcg cggacctcgc
     3841 gatgctcgac gatcgcgcgg tgttcggcgc ggcggatgcg ctgcacgcag tacgtgcact
     3901 tctccatcac gccgcgcgag cgcaccgtga catccgggtt gtattgcagc gcgcgcgtgg
     3961 aggtctgctc gtacgcgaac cagttgaagc ggcggacctt gtagggacag ttattcgagc
     4021 agaaccgcgt gccgatgcag cggttgtaga cctgctcgtt gagaccgtcg gggctatgcg
     4081 tcgtcgcgtt gaccgggcac gcgtattcgc acggcgcgta ttcgcagtgc tggcacggca
     4141 tcggctcgtt gacgacgacg ccgtcctcgc gcacgtagcg atcgatgcgg atccagttca
     4201 tgtggcggcc gcgggcgaca tcgcgtggac cgaccgtcgc gatgttgttc tcggcctgac
     4261 acgcgaccat gcatgcgctg cagccggtgc agatcgaggt atcgatcgac ataccccact
     4321 gcgtgccgtc gcgcggttgc ggcggcagta gcgacgcctg ctcgccgcgc agatgagcaa
     4381 cgaagttggg ttgcgcagcg aacgcggcga gcgttgccgc tggcgcgatc tcgcggccgt
     4441 gctcggcgaa cgtcatctgc gtgcgcggca ggaccgtctt gccggcgagc acgcgcacgg
     4501 cgccatggag cagcaggcga tcgtcggtgc gcagcgcgta gccgtcgccg ccgatctggt
     4561 cggcgatcgg cagctgcggg acgtgttggc catagccgag ctcgacggtg atcgaaccat
     4621 ccgcagcgcc gggcaccacg atcaccggca acgcgagctg cccggcggtc gtcgtcagct
     4681 cgagccggct gccatcggcg acaccgagcc ggtc
//
LOCUS       DASUFX010000726         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1784132, whole genome shotgun sequence.
ACCESSION   DASUFX010000726 DASUFX010000000
VERSION     DASUFX010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784132"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..758
                     /locus_tag="VFV99_29385"
     CDS             63..758
                     /locus_tag="VFV99_29385"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5063540.1"
                     /translation="MHKVDAKMEAGERPDQSIVQRMGQLVGRSLKAGIFKDGAGLHRS
                     ATRARITFNGGKPSITRGPYAGGNELTARFALISTTGIEKAIELATQLGEAAGQREIE
                     IGPVVEGWDLNGSPRPADAPHRFLLLVKADAAFEAGAPQPAAVNTLLARWKRDGVLQS
                     DISMKSSKTSVRFKAVSGKRTWLDGPFTESKELIAGFSVIEVDSLDDAKKFTEEYAAI
                     LGDNEVDIREVAV"
     gene            908..2401
                     /locus_tag="VFV99_29390"
     CDS             908..2401
                     /locus_tag="VFV99_29390"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEX5063541.1"
                     /translation="MKHHAFQLVAAGIVLASGAASASPRSRMGEVDYRADGSVRVGTT
                     VFSTREAFHRSTLFRDSGARCGTPEHEPVMELISQSDCTLNTTHINQDYNDSRTLVIP
                     VVFHVIKKTDGTTGNISAALLQSQVDILNEDFNAMPSTNGSMGTNIKLQFVLAKLDPA
                     GNPTTGIDYVTNNTYFNDPGSGTNAMKRALKWDPTRFLNIYTNDAGGGGTLGYATFPQ
                     EEAGGQEDGVVLNWVYVGRNAPGGAPYNLGRTATHEVGHYLGLFHTFQSGCGTTTAPY
                     TSGDLIGDTAREAQPNYGCSPVSSGCGGNSPIENYMDYSEDSCMTKFTVEQANRIRCS
                     IINYRWINTPPAAGFTVTTSGYDATFTNTSMDAQTAAAMLKYKWTFGDGATSTDQNPV
                     HTYATDGTYNVTLEVLDPGSGTNSKTQSVMIMGAATTPDAGVGSGSDQPDGGNGGGGG
                     GGGGGGGNDQPGGMSGGCCDAPGSGTSALLCGIPVVLVVVRRRRTRR"
     gene            2437..>2749
                     /locus_tag="VFV99_29395"
     CDS             2437..>2749
                     /locus_tag="VFV99_29395"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5063542.1"
                     /translation="MTGSYWQRDGGWCDRWIAAPLPAATDFVVLGGGFAGLMTAIRLR
                     EQNPTATVAVLEAERVGYGASGRNAGFLSPLAAPVWLLGAERSAEQAWGAARINGEVH
                     AI"
BASE COUNT          522 a          888 c          904 g          435 t
ORIGIN      
        1 gatctagccc gcgctcgatc gtcgtctctt caggaggtca gcaaattatg cgtttcatgg
       61 tcatgcacaa agtcgatgcg aagatggagg ctggcgagcg gcccgaccaa tccatcgtcc
      121 aacggatggg gcagctcgtc gggcgctcgc tcaaagccgg catcttcaaa gatggcgccg
      181 ggctacatcg gagcgcgacg cgcgcgcgta tcacgttcaa cggcggcaag ccgtcgatca
      241 cgcgcggccc ctacgccggt ggcaacgagc tgaccgcgcg atttgcactg atctcgacga
      301 ccggcatcga gaaagcgatc gagctcgcaa cgcagctcgg cgaggccgct ggtcaacgcg
      361 agatcgagat cggtcccgtc gtcgagggtt gggatctcaa tggcagcccg cgccccgccg
      421 atgcgccaca ccggttcttg ctactcgtca aagccgacgc cgcgttcgaa gccggtgcgc
      481 cgcagcccgc cgcggtcaac acgttgctcg cgcgttggaa acgcgacggc gtgctgcaat
      541 cggacatctc gatgaagtcg agcaagacct ccgtgcgctt caaagcagtc agcggcaagc
      601 gcacgtggct cgatggaccg ttcaccgaat cgaaggagct gatcgcaggc ttctccgtca
      661 tcgaggtgga ctcgctcgac gacgcgaaga agtttaccga ggagtacgca gcgatcctcg
      721 gcgacaacga ggtcgacatt cgcgaagtcg cggtctgagc acgaaatacc ggcgctggtg
      781 tctgcgtgct gacatcagcg atgcgcgtga tgcgcacgat gcgatgcgcg cagtacgacg
      841 cgactgccag gagctttgag cacacctggt gcgaggttcg ccgcgcgcgc tcataggatt
      901 gtcggcgatg aagcaccacg catttcagct cgtggcggcg ggtatcgtgc tcgcatcggg
      961 cgcggcctcc gcttctcctc gctcgcgcat gggcgaagtc gattaccgcg ccgacggttc
     1021 ggttcgcgtc ggaacgacgg tattcagcac gcgcgaggca tttcatcgct cgacgctgtt
     1081 tcgcgacagc ggcgcgcgct gcggtacgcc cgagcacgag cccgtgatgg agctgatctc
     1141 gcagagcgac tgcacgctca acacgacgca catcaaccag gactacaacg acagccgcac
     1201 gctcgtgatc ccggtcgtct tccacgtcat caagaagacg gacggcacga ccggcaacat
     1261 ctcggcggcg ctgctccaga gccaggtcga tatcctgaac gaagacttca acgcgatgcc
     1321 cagcacgaac ggctcgatgg gcacgaacat caagctgcag ttcgtgctcg cgaagctcga
     1381 tccggcgggc aacccgacga ccggcatcga ctacgtcacg aacaacacgt acttcaacga
     1441 tccgggcagc ggcacgaacg cgatgaagcg cgcgctcaag tgggatccga cgcgcttcct
     1501 caacatctat acgaacgacg cgggcggcgg cggcacgctc ggctacgcga cgttcccgca
     1561 agaagaagcg ggcggacaag aagacggcgt cgtcctcaac tgggtctacg tcggtcgcaa
     1621 tgcgcccggc ggcgcgccgt acaacctcgg tcgcacggcg acgcacgagg tcggccacta
     1681 cctcggcctg ttccacacgt tccagagcgg ctgcggcacc acgaccgcgc cgtacacgag
     1741 tggcgatctg atcggcgaca ccgcgcgcga agcgcaaccg aactacggat gcagcccggt
     1801 gtcctcgggt tgcggcggca acagcccgat cgagaactac atggactaca gcgaagactc
     1861 gtgcatgacg aagttcaccg tcgagcaggc caatcgcatc cgctgctcga tcatcaacta
     1921 tcgctggatc aacacgccgc ctgccgccgg gttcaccgtc acgacgagcg gttacgacgc
     1981 gacgttcacg aacacgagca tggacgcgca gaccgcggcc gcgatgctca aatacaagtg
     2041 gacgttcggc gatggcgcaa cgtccaccga tcagaacccg gtgcacacgt acgcgaccga
     2101 cggcacgtac aacgtcacgc tcgaggtcct cgatcccggc agcggcacga attcgaagac
     2161 ccagtcggtg atgatcatgg gtgctgcgac gacgccggat gccggcgttg gcagcggcag
     2221 tgaccagcca gacggcggaa atggcggcgg tggcggtggt ggcggtggtg gtggtggcaa
     2281 cgaccagccg ggtggcatgt ccggtggttg ctgcgatgcg ccgggtagcg gtacgtctgc
     2341 cctgctctgc ggcattccgg tcgtgctcgt ggtcgtgcgt cgtcgtcgca cgcgccgcta
     2401 gcgcaaccgc gccgctgcgt cggttacgct cgcctgatga cggggagcta ctggcagcgc
     2461 gatggcggct ggtgcgatcg gtggatcgcc gccccgctgc ctgccgcgac cgactttgtc
     2521 gtgctcggcg gtggattcgc cggtctgatg accgcgatcc ggctgcgcga gcaaaatccg
     2581 acggcgacgg ttgcggtact cgaggccgag cgcgtcggct acggcgcgag cggacgcaat
     2641 gccgggtttc tgtcgccgct cgccgcgcca gtatggctgc tcggtgccga gcgctcggcg
     2701 gaacaagcgt ggggcgccgc gcgcatcaat ggcgaagtac acgcgatcg
//
LOCUS       DASUFX010000727         8238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_951112,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000727 DASUFX010000000
VERSION     DASUFX010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951112"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /gene="uvrA"
                     /locus_tag="VFV99_29400"
     CDS             <1..1466
                     /gene="uvrA"
                     /locus_tag="VFV99_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5063543.1"
                     /translation="LSGGEAQRIRLASQLGSELSGVLYVLDEPSIGLHQRDNERLIRT
                     LHRLRDLGNTVLVVEHDEATIEAADWVVDFGPGAGRHGGHVVAEGTPEDIKQVKESIT
                     GRFLAGTETIEVPESRREPSSWVKLTGAREHNLKNVTVKIPLGVMVAVTGVSGAGKSS
                     LINATLYPALNRMLHRSLDRVGPYDELTGLGQVDKCIVIDQQPIGRTPRSNPATYTKA
                     FDLIRELFAQVPQARTYGYQAGRFSFNVSAKQGGGRCEACEGAGVREVEMHFLPNVFV
                     TCEVCKGRRYNDATLRVKYKDKDIAQILDTPVEEAYEVFKHHKQLGRIMQTMIDVGLG
                     YLSLGQPATTLSGGEAQRVKLARELARVQTGRTLYLLDEPTTGLHFGDVKKLLEVLNR
                     LVDNGNTVLVIEHNLDVIKTADWIIDLGPEGGAAGGEIIAAGTPEDVAQIARSYTGQH
                     VKPLLERGKRGRSSSANGGGNGRSSRRERRGGLEASP"
     gene            1598..3601
                     /locus_tag="VFV99_29405"
     CDS             1598..3601
                     /locus_tag="VFV99_29405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5063544.1"
                     /translation="MVVALEPELDGDARKVLSQLPYAVVVVDRDWRITLANAEAHRLL
                     GQQGTTLWDLCPELEATPFGTAFRYAMTDRAELISESALPAAGWLQARAKPFDDGLLI
                     TLRPVHPETSTDLQAKQALLLGEVGMALTGASTLREMLQRCADAMVRRLDAALARIWT
                     LDDAYRELVLEASSGVAEPDTLERVPIGRSKIGKIVERGAPHLTNDFQHDTRGTNREW
                     AARERITAFAGYPLRVDGNVVGVLTVYSRHKLGHDTTGGLATIADSIALGIERKGSDD
                     ARQSAERELRAQADRLELINEIGKNLTSELELAPLVQRVTNLAARLAHATFGAFFFDY
                     DAAHDQFTKFAVSGAPRDAIAIPLRPTQVGPMTLGKLPTASFMAMPVIARGGKLVGAF
                     AFGHEQAGMFNEATERLLAGVAAQAAIAMDNAQLFASARELISQLEKTNAELDQFAYV
                     ASHDLKAPLRGIANLAQWIEDDLGDKMDEEGRYHMGLLRGRVSRLENLIEGILAYSRV
                     GRDEEAAVDIDIATFVREIWELISAQPTSKLTVGELPRLVTQKVPLQQVLMNLMSNAV
                     KYNAGKVHIEVGARKHGDAWAIYVKDDGVGIPPEFQQRIWGLFQTLEPRDKVESTGIG
                     LAVVRKIVEARGGRAWVESEAGTGATFWFTWPADCSTRKKRHG"
     gene            3594..4010
                     /locus_tag="VFV99_29410"
     CDS             3594..4010
                     /locus_tag="VFV99_29410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5063545.1"
                     /translation="MDDRVINILLVDDDDVDVMTVKRAFAKNNISNPLFVAHNGLEAL
                     ELLRKEDIPARRRLILLDVNMPKMNGIEFLREVRSDPKLQQASVVMMTTSNEERDRIE
                     AFKLNVAGYLLKPVTFQQFAEVMSTINKYWALMEMP"
     gene            complement(4051..4701)
                     /locus_tag="VFV99_29415"
     CDS             complement(4051..4701)
                     /locus_tag="VFV99_29415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063546.1"
                     /translation="MMRALFSTLLLVSLTATAIAQAPGQPYPQPQPQPYPQPQPYPQQ
                     PQPYGPQYGYAQRPVMQYQLTVDEQFLLQRGFISDGETFGGGLAQLFFGFGIGQAIQG
                     RWSQDGWIFTLGEGASSVFFVYGMTKFLTECFEAPDCHENDGVGMMVAGMLGVTVFRI
                     WGTIDAFAVPSSHNRKVRELRMRLGLPVPMYARVAPYVSPVKGDQGGGVAGVSFRF"
     gene            complement(4720..5889)
                     /gene="epmA"
                     /locus_tag="VFV99_29420"
     CDS             complement(4720..5889)
                     /gene="epmA"
                     /locus_tag="VFV99_29420"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX5063547.1"
                     /translation="MGTRGRVIAIDGDAAIVRAELFDLRITNPAKDWRPGDLVDVDRP
                     AVLRTHPGGDYPAPGSEVMRLPRARVDKLRARATALAAVRSGFASRGFLEVETPLLVP
                     SPGLEIHLEAVRAGDGYLITSPEYQMKRLLAAGFERIYQVCKCFRANERGAHHASEFT
                     MIEWYRTFAGIDSIVDDTEQLVSAVVRSITGVPVANVSGRDIDVRPPWRRMTVRAAMS
                     EYAGVAVDGAEPAGDLVRAVRAAGIEVADALAWDDAFFAAFLARVEPAIAALDHALIL
                     EDWPAPLAALARRKPDDPKTALRFEAYVGGIELANAFDELTDSDEQRSRFIEDQRVRR
                     ERGKPVYPIDERLLAALHEGLPPSAGIALGFDRLVMIATGAESIEEVLTFTADEL"
     gene            complement(5889..6455)
                     /gene="efp"
                     /locus_tag="VFV99_29425"
     CDS             complement(5889..6455)
                     /gene="efp"
                     /locus_tag="VFV99_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5063548.1"
                     /translation="MYDTSDIRKGLKVLMDGNPYTVVEFQFVKPGKGAAFTRTKFKNL
                     LTGAVIERNIRSGEKLEPANVETRDMQFLYKEANDLVFMDQTNYEQVTISKDLIGDAH
                     DLMKDNLPCTVVFFNERPVDITLPTFVFLEVTGGEPGVAGNTAAGNVTKSATVETGAE
                     VQVPLFIRVGDTIKIDTRTHEYVERVTK"
     gene            6563..7045
                     /locus_tag="VFV99_29430"
     CDS             6563..7045
                     /locus_tag="VFV99_29430"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5063549.1"
                     /translation="MRLGREEFLASAAPAALVRHKKDFEDAPHSGVHTLTIDQEIEET
                     LPHGRESPTVMSNLELFPLIKKPGASFPDRITIGRTVNNDIVIPDSSISRLHAYLRRD
                     ANGWVLADAGSKNGSWLEGKTLAARKELALRSRAELRLGDVDLTFYLAADLFTALGGA
                     "
     gene            7042..7389
                     /locus_tag="VFV99_29435"
     CDS             7042..7389
                     /locus_tag="VFV99_29435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063550.1"
                     /translation="MSEWDQRQLCPDGACVGVIGDDGTCKVCGRAAPNWGDERNRGLV
                     DENDHSDESQPPPESVTKSDKPLLYFDVDKTDEGDAKPDADWDQRKLCPDDACIGVIG
                     ADGTCKVCGRSAA"
     gene            7386..8036
                     /locus_tag="VFV99_29440"
     CDS             7386..8036
                     /locus_tag="VFV99_29440"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5063551.1"
                     /translation="MTRATIVGAGGLGGPLALSLGAAGIDLTIIDPDIVEESNLHRQI
                     QFAIADVGKPKAPLLAAAVVARGGRARGYQTRWTADDADDISGDSDLIVDGSDDPDTK
                     FAVADWAVARGKPYVIAAALRYGGNVLVGAPGSSCYRCLFEEPVAAATCAAAGVLGPV
                     VGAIGGLAGALAIGLANSDRSHAGSIFVFDDLRASQEPRIVRFGQRRGCSTCVRAA"
     gene            8046..>8238
                     /locus_tag="VFV99_29445"
     CDS             8046..>8238
                     /locus_tag="VFV99_29445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063552.1"
                     /translation="MPMIRIPTALRTLTGGNADIQVSAGTVRDALVELDRRHPGVAAK
                     VLDAAGAVKPFIRIYVRAED"
BASE COUNT         1477 a         2715 c         2734 g         1312 t
ORIGIN      
        1 ccctgtcggg cggcgaggca cagcgcatcc ggctcgcgtc gcagctcggc agcgagctgt
       61 cgggcgtgct ctacgtcctc gacgagccgt cgatcggtct gcaccagcgc gacaacgagc
      121 ggttgatccg cacgctgcat cgcctgcgcg atctcggcaa caccgtgctc gtcgtcgagc
      181 acgacgaggc gacgatcgaa gcggcggact gggtcgtcga cttcggcccg ggcgcgggcc
      241 gacacggcgg tcacgtcgtc gccgaaggca cgccggagga catcaagcag gtcaaggaat
      301 cgatcaccgg tcgcttcctc gccggtaccg aaacgatcga ggtgccggag agccgccgcg
      361 agccgtcgag ctgggtgaag ctgaccggcg cacgcgagca caacctcaag aacgtcacgg
      421 tgaagattcc gctcggcgtg atggtcgcgg tcacgggcgt gtcgggcgcg ggcaagtcgt
      481 cgctgatcaa cgcgacgctg tacccggcgc tcaaccgaat gctgcaccgc tcgctcgatc
      541 gcgtcggtcc gtacgacgag ctcaccgggc tcggccaggt cgacaagtgc atcgtgatcg
      601 atcagcagcc gatcggccgc acgccgcgca gcaacccggc gacgtacacg aaggcgttcg
      661 atctgatccg cgagctgttc gcgcaggtgc cgcaggcgcg cacgtacggc tatcaggccg
      721 gccgcttctc gttcaacgtg agcgcgaagc aaggcggcgg tcgctgcgag gcgtgtgaag
      781 gcgccggtgt ccgtgaagtc gagatgcact tcctgccgaa cgtgttcgtc acgtgcgagg
      841 tctgcaaagg ccgtcgctac aacgacgcca cgctgcgcgt gaagtacaag gacaaggaca
      901 tcgcgcagat tctcgacacg cccgtcgagg aagcgtacga ggtgttcaag catcacaagc
      961 agctcggccg catcatgcag acgatgatcg acgtcggtct cggctacctg tcgctcggtc
     1021 agccggcgac gacgctctcg ggcggtgagg cgcagcgtgt gaagctcgcg cgtgagctcg
     1081 cccgtgtgca gaccggccgc acgctgtact tgctcgacga gccgaccacc ggccttcact
     1141 tcggcgacgt caagaagctc ctcgaagtgc tgaaccgcct cgtcgacaac gggaacacgg
     1201 tgctcgtgat cgagcacaac ctcgacgtca tcaagacggc ggactggatc atcgatctcg
     1261 gtcctgaagg tggcgctgca ggtggcgaga tcatcgctgc gggtacgcca gaagacgtcg
     1321 cacagatcgc gcgcagctac accggccagc acgtgaagcc gctactcgag cgcggcaagc
     1381 gcggtcgatc gtcgtcggca aatggcggcg gcaatggtcg ctcgtcgcgc cgcgagcgtc
     1441 gcggtgggct cgaagcgtcg ccgtgaatac ggacacgcac cgcgccggtt cgcacgtcga
     1501 gcgctgacgc acgctcccgc cccgtaaggt gcgcgcctta gggcgtctta acgttagctc
     1561 gacatggacg catgattgca gatgcgtatc gtccgccgtg gtcgttgcgc tagagccaga
     1621 gctcgacggc gacgcacgca aagtgctgtc gcaactgccg tatgcagtcg tcgtcgtcga
     1681 tcgcgactgg cgaatcaccc tggcgaacgc cgaagcgcat cggctactcg ggcagcaagg
     1741 aaccacgctg tgggacctgt gtcccgagct cgaagcgacg ccgttcggga ccgcatttcg
     1801 ttatgcgatg acggatcgcg cggaattgat cagcgagagc gcactgcctg ctgccggctg
     1861 gctgcaggcg cgtgccaagc cgttcgacga cggcctgctg atcacgctgc gcccggtcca
     1921 ccccgagaca tctaccgacc tgcaggcaaa gcaagcgttg ctcctcggcg aggtcggtat
     1981 ggcgctgacc ggcgcgagca cgttgcgcga gatgttgcag cggtgcgccg acgcgatggt
     2041 gcgtcgcctc gacgcggcgc ttgcgcggat ctggacactc gatgacgcgt accgcgagtt
     2101 ggtgctcgaa gcgtcgtctg gcgtggccga gcccgacacg ctcgagcgcg tgccgatcgg
     2161 ccgctcgaag atcggcaaga tcgtcgaacg tggcgcgccg cacctgacga acgacttcca
     2221 gcacgacaca cgcgggacaa accgcgagtg ggcggcgcgt gagcgcatca cggcattcgc
     2281 cggctatccg ctgcgcgtcg acggcaacgt cgtcggcgtg cttacggtgt acagccgcca
     2341 caagctcggc cacgacacga ccggcgggct tgcgacaatc gccgactcga tcgcgctcgg
     2401 catcgagcgc aagggctccg atgacgcgcg ccagtccgcc gaacgcgagc tccgtgcgca
     2461 agcggaccgg ctcgagctga tcaacgagat cggcaagaat ctaacctccg agctcgagct
     2521 cgcaccactc gtccagcgcg tgacaaacct cgcggcgcgg ctcgcacacg cgacattcgg
     2581 cgcgttcttc ttcgactacg acgccgcgca cgatcagttc acgaaattcg ccgtcagcgg
     2641 cgcgccacgc gatgcgatcg cgatcccgct ccggccgaca caggtcggcc cgatgacact
     2701 cggaaagcta ccgaccgcaa gcttcatggc gatgccagtg atcgcgcgcg gcggcaagct
     2761 cgtcggcgcg ttcgcgttcg gccacgagca ggcgggcatg ttcaacgaag cgaccgagcg
     2821 gttgctcgcg ggtgtcgcgg cgcaggcggc gatcgcgatg gataatgccc agctgttcgc
     2881 gtccgcgcgc gagctaatca gccagctcga aaagacgaac gcagagctcg atcagttcgc
     2941 gtatgtcgcc agtcacgacc tcaaggcacc gctgcgcggc atcgcgaacc tggcccagtg
     3001 gatcgaggat gacctcggcg acaagatgga cgaggaaggc cgctatcaca tggggctgtt
     3061 gcgcggtcgc gtgtcccggc tcgagaactt gatcgaaggc attctcgcgt acagccgcgt
     3121 cggccgcgac gaggaagcgg cggtcgacat cgacatcgcg acgttcgtgc gggagatctg
     3181 ggagctgatc tcggcgcagc cgacgtcgaa actcacggtc ggcgaattgc cgcgcctcgt
     3241 gacccagaag gtcccgctgc agcaggtgct gatgaacctg atgtccaacg cggtgaagta
     3301 caacgccggc aaggtccaca tcgaggtcgg tgcacgcaag cacggcgacg cttgggcgat
     3361 ctacgtcaaa gacgacggcg tcggcatccc gcccgaattc cagcagcgga tctggggcct
     3421 gttccaaacg ctagagccgc gcgacaaggt cgagagcacc ggcatcggtc tcgcggtggt
     3481 tcgcaagatc gtcgaggcgc gcggtggccg cgcctgggtc gagtccgaag ctggcacggg
     3541 cgcgacattc tggttcacgt ggccagcaga ctgcagtacg aggaagaaac gtcatggatg
     3601 atcgcgtgat caacatcttg ctcgtcgacg acgacgacgt cgacgtgatg accgtcaagc
     3661 gcgcgttcgc gaagaacaat atctcgaacc ccctattcgt cgcccacaac ggactcgaag
     3721 cactcgagct gctgcgcaag gaggatattc cggcacggcg ccggctgatc ctgctcgacg
     3781 tcaacatgcc gaagatgaac ggcatcgagt ttctccgcga ggtgcgctcc gatccgaagc
     3841 tgcagcaggc gagcgtcgtg atgatgacga cgtcgaacga ggagcgcgat cgcatcgaag
     3901 cgttcaagct caatgtcgcc ggttacttgc tgaagcccgt cacgttccag caattcgcgg
     3961 aagtgatgtc gacgatcaac aagtactggg cgctgatgga aatgccataa ctcccagctg
     4021 tgcttcggcg atgatgcgcc gccacgagcg ctagaagcgg aacgacactc cggcgacgcc
     4081 gccgccctga tcgcctttca cgggcgatac gtacggcgcc acgcgcgcgt acatcgggac
     4141 cggcaggccg agccgcatgc gcagctccct caccttgcgg ttgtgcgacg acggtactgc
     4201 gaacgcatcg atcgtgcccc agatccggaa cacggtgacg ccgagcatgc cggcgaccat
     4261 catcccgaca ccgtcgttct cgtgacagtc gggcgcctcg aaacactcgg tgaggaactt
     4321 cgtcatgccg tagacaaaga aaacactcga ggcgccttcg ccgagcgtga agatccaacc
     4381 gtcctgcgac caccggccct ggattgcctg gccgatgccg aagccgaaga acagctgagc
     4441 tagcccgccg ccaaaggttt caccgtcgga gatgaagccg cgctggagca agaactgctc
     4501 gtcgaccgtg agctggtact gcatcacggg ccgctgcgcg tagccgtact gcggcccgta
     4561 gggctgcggc tgctgcgggt acggctgcgg ttgaggatag ggttgcggct gcggttgcgg
     4621 atacggctgc cccggcgcct gcgcgatcgc agtggcggtc aacgagacga ggagcaacgt
     4681 cgagaacagt gcgcgcatca cgacccccga gcgtcgatac tacagctcgt cggcggtgaa
     4741 cgtcagcact tcctcgatcg actccgcgcc tgtggcgatc atcacgagac gatcgaatcc
     4801 gagtgcgata ccggcagaag gtggcagacc ctcgtgcagg gcggcgagca gcctctcgtc
     4861 gatcggatac accggcttgc cgcgctcccg tcgcacgcgt tgatcctcga tgaaccgcga
     4921 gcgctgctcg tcggaatcgg taagctcgtc gaacgcgttc gcaagctcga tgccgccgac
     4981 gtacgcctcg aaccgcagcg cggtcttcgg atcgtcgggc ttgcgacgcg cgagtgcggc
     5041 gagcggcgcc ggccagtcct cgaggatcag tgcgtgatcg agcgccgcga tcgcaggctc
     5101 gacccgcgcg aggaacgccg caaagaacgc gtcgtcccac gcgagcgcgt ccgcgacctc
     5161 gatgccggct gcacgcaccg cgcgcacgag atcgccggca ggctcggcgc catcgaccgc
     5221 aacacccgcg tactcgctca tcgccgcgcg cacggtcatg cgtcgccacg gcggacgcac
     5281 gtcgatatcg cggccgctga cgttggcgac cggcacgcca gtgatcgagc gaacgaccgc
     5341 cgagacgagt tgctcggtgt cgtcaacgat cgagtcgatg cccgcgaacg tgcggtacca
     5401 ctcgatcatc gtgaactcgc tcgcgtggtg cgcgccgcgc tcgttggcgc ggaagcactt
     5461 gcacacctgg tagatccgct cgaagccagc tgcgagcagc cgcttcatct gatactcggg
     5521 cgacgtgatc agatagccgt cgccggcgcg cacggcttcg aggtggatct cgaggcccgg
     5581 gctcggcacg agtagcggcg tctcgacttc gaggaacccg cgcgacgcaa acccggagcg
     5641 aaccgcggcc agcgccgtcg cacgcgcacg cagcttgtcg acgcgcgcac gtggtaaccg
     5701 catcacctcg ctgcccggcg ctggatagtc gccaccggga tgcgtccgca gcaccgcggg
     5761 acgatcgacg tcgacgagat cgccgggccg ccaatccttg gcgggattcg tgatccggag
     5821 atcgaacagc tcggcgcgca cgatcgcggc gtcgccgtcg atcgcgatga cgcggccgcg
     5881 cgtccccact acttggtcac gcgctcgacg tactcgtgcg tgcgcgtatc gatcttgatc
     5941 gtgtcaccga cacggatgaa cagcggcacc tggacttcgg cgccggtctc gaccgtcgcg
     6001 gacttggtca cgttgccggc cgccgtgttt ccagcgacgc cgggctcgcc gcccgtgacc
     6061 tcgaggaaca cgaacgtcgg cagcgtgatg tcgactggac gctcgttgaa gaacaccacc
     6121 gtgcacggca ggttgtcctt catcagatcg tgcgcgtcgc cgatcaggtc cttcgagatc
     6181 gtaacctgct cgtagttcgt ctgatccatg aagaccagat cgttggcctc tttgtagagg
     6241 aactgcatgt cccgggtctc gacgttcgcg ggctcgagct tctcgcccga ccggatgttc
     6301 cgctcgatca cggctcccgt cagcaagttc ttgaacttgg tacgagtaaa cgccgcgccc
     6361 ttgccgggct tgacgaactg gaactcgaca acggtgtagg ggttcccgtc catcaagacc
     6421 ttgaggccct tgcggatatc cgaggtatcg tacattgcgg gttcgtttac cccgtgccct
     6481 ctggggctgt caaacgcttg ccgctcggcc ctgcgtatgc gacatcctcg ccgtggatgc
     6541 tgcggcgcac tatcgccagc ttgtgcggtt gggacgtgag gagttcttgg cctcggcggc
     6601 acccgccgcc ctggtccgtc acaagaagga cttcgaggat gcgccccata gcggcgtgca
     6661 caccctgacg atcgaccagg agatcgagga gacgctcccg cacgggcgcg agtccccgac
     6721 cgtgatgtcg aacctcgagc tgtttccgct gatcaagaag ccgggggcct cgttcccgga
     6781 ccggatcacg atcggccgta ccgtgaacaa cgacatcgtg atcccggatt cgtcgatctc
     6841 gcggctgcac gcgtatctgc ggcgcgacgc aaacggctgg gtcctcgccg acgccggctc
     6901 gaagaacggc agctggctgg aaggcaagac gctggccgcg cgcaaggagc tcgccctgcg
     6961 gtcgcgggca gagctgcggc tcggcgatgt cgatctcacg ttctatctcg cagcggatct
     7021 gttcaccgca cttggtggcg catgagcgaa tgggatcaac ggcagctgtg cccggatggc
     7081 gcctgcgtcg gcgtgatcgg tgacgacggc acgtgcaagg tgtgcggccg tgccgcgccg
     7141 aactggggcg acgagcgcaa ccgcggcttg gtcgacgaga acgatcactc cgacgagtcg
     7201 caaccgccgc cggaatcggt gacgaagagc gacaagcccc tgctctactt cgacgtcgac
     7261 aagaccgacg agggcgatgc caagcccgac gccgactggg accagcgcaa gctgtgcccc
     7321 gacgacgcgt gcatcggcgt catcggcgcg gacggcacct gcaaggtgtg cgggcggagc
     7381 gcggcgtgac gcgtgcgacg atcgtcggtg cgggcgggct cggtggtcca ctcgcactgt
     7441 cgctcggcgc agccggcatc gacctcacga tcatcgatcc cgacatcgtc gaggaatcga
     7501 acctccatcg ccagatccag ttcgcgatcg ccgacgtcgg caagccaaag gcaccgctgc
     7561 tcgctgccgc ggtcgttgca cggggcggac gagcgcgtgg ttatcagacg cgctggaccg
     7621 ccgacgacgc cgacgacatc tccggcgaca gcgacttgat cgtcgacggc agcgacgatc
     7681 cggacaccaa gttcgcggtc gccgactggg cagtcgcgcg cggcaagccg tacgtgatcg
     7741 cggcagcgct gcgctacggc ggcaacgtgc tcgtcggggc gcccggcagc agctgctatc
     7801 gctgcttgtt cgaggaaccg gttgcagccg caacgtgtgc ggcggcgggc gtgctcggtc
     7861 cggtcgtcgg cgcgatcggt ggcctcgcgg gcgcgctcgc gatcggcctc gcgaacagcg
     7921 accgttcgca tgccggctcg atcttcgtgt tcgacgatct gcgcgcgtcg caagagccac
     7981 gcatcgtcag gttcggccag cggcgtggct gttccacgtg cgtgcgcgct gcctaggagt
     8041 caccgatgcc gatgatccga atcccgactg cactgcgaac gctcaccggc ggcaacgctg
     8101 acatccaggt cagcgcaggc acggtccgcg acgcgctcgt cgagctcgat cgccgtcacc
     8161 cgggtgtcgc cgccaaggtg ctcgacgcgg cgggcgccgt gaagccgttc attcgcatct
     8221 acgtccgggc cgaggata
//
LOCUS       DASUFX010000728         6027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_534886,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000728 DASUFX010000000
VERSION     DASUFX010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534886"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFV99_29450"
     CDS             complement(<1..555)
                     /locus_tag="VFV99_29450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063553.1"
                     /translation="MKLRFVSVALSAVACSASPPLPPARFVNANPVRVVDDRRNVAIP
                     PKSIGPETDLSEFNSAVVRPIVRPLELKPHRRALGVNSIDEVPDSTWFTNRIGVRELT
                     PDEVRQGPATIEGPEAYVPWTVVGSKREGVSQGVFIVDSRGERYLIKFDKKGVPELES
                     GGHVITNRLLWAAGYNVPEDSVAYI"
     gene            complement(552..1958)
                     /locus_tag="VFV99_29455"
     CDS             complement(552..1958)
                     /locus_tag="VFV99_29455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5063554.1"
                     /translation="MKHAISIAILLSVSSAQAQPADEPPTPVEQETEQPVAPIEYQPP
                     PGEESGRIDEPSDGDSAGRKVARTLLYIPRGIVALLLLPARGAILANDKWAIVPTARR
                     WLFNDDMTIGVYPLLFFESTQGVMLGAQFVAELTPNDYIKLFAGAGTASRLRTSATYQ
                     MTNRLDGRLDSQFDVEYDVRGQSRFYGFGNADQVDFEPVMPVDPFDEPVAFVSYYKDK
                     LARASAVGNVEVLRDFYVDGGASVARRDREDSSKGPPIQDVYMPSSLIAFDHYNALYT
                     EGQLRYDSRGPTSQWQPQMLWSAGTLAAVYGGITALDTGRNFFRYGFDLQQIFPIGGN
                     PRVLSARVHGEAITAENNEVPFNEVPSLGGRQWLRGYATDRFRDKIALAGSLEYQWDL
                     ARWLYASVYADVGHVYPAWDDVTLAGMRCGYGFSLEVQTHTSLVGRLSVSSSIDGDVW
                     FNLYLDPVSAIEPRVRRR"
     gene            complement(1955..3202)
                     /locus_tag="VFV99_29460"
     CDS             complement(1955..3202)
                     /locus_tag="VFV99_29460"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEX5063555.1"
                     /translation="MLLLLALFLLLTSFYVLKTAREGIVLASTTFRLQGDEVKTYASG
                     AMALLLVVLVPAYDMLANRLRRIALVNISIAFVVISLAGFYVAAHAGVSIGLAFYVWL
                     GVVSVFLIAQFWSFANDFYSEEQGKRVFAMIALGGSMGAIAGPRLAQIATTRDLMLLS
                     PILLFAYAMLINIVDRCDDHQRHERIVKEVDADAGGFALVLRDPYLALIAVLLLILSL
                     VNTTGDYILSNAVLERAMQIAPDPHATAERSELIKTFYGDFFFHVNLVAFALQAAFVS
                     RVIRKLGVGRALFVLPVIVLGAYGSVAMLGTFAVVRAAKLTEGSTDYSLHNTVRQMLF
                     LPVTRAAKYKAKAAIDTFFVRFGDTLSAVLVAVAIHALAFGRRALAIVNVALVAVWLV
                     ICVAIARRHQALTHRPLIAGELG"
     gene            complement(3331..3876)
                     /locus_tag="VFV99_29465"
     CDS             complement(3331..3876)
                     /locus_tag="VFV99_29465"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5063556.1"
                     /translation="MRRLALLALAGCALTSKSPPLDVHYYTPDVSPTTQAYALPSDTK
                     IRLGRVSAASHLRARIAYRVSPTQIEFYETRRWAESPEAYARRALEQVLAERGVLVTG
                     GKALQLDVEVVTFEEVRDPRPLARVGLRYTLIDNRVVIREGTVETEHTAGPSFDSFVV
                     AIGQALDDASLQIASESLTAN"
     gene            complement(3879..4706)
                     /locus_tag="VFV99_29470"
     CDS             complement(3879..4706)
                     /locus_tag="VFV99_29470"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5063557.1"
                     /translation="MKSTSRHVKLGLLALLAAAAVVSAMLALGLRKKPTDVYHTYFNE
                     SVQGLDEGAIVKFRGVRIGKVKSISVAPDRRLIDVGMAIDRSRAAELRLDKSALTLRA
                     RLVIFGITGVKLIDLDFADEHTPPAPELTFKPPARYIPSEMSLLGSLEDDVVTFAHRL
                     PMIADHAIAALDAVHHVTYDARSLLEETRDTVTAIGYVARGAARADLPKSIAKTLGTI
                     NGTLDELGQRTTDATGELESTIRDIGDAARQLRDFLDTLEREPDILIKGRYRPGRRR"
     gene            complement(4703..5428)
                     /locus_tag="VFV99_29475"
     CDS             complement(4703..5428)
                     /locus_tag="VFV99_29475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5063558.1"
                     /translation="MIEAHDLTIGWSEDEILLEHASFVVERGEIFAILGRSASGKSTL
                     LRVLIGLERPRAGEVLVLGRPPAFRAEAPEFGVLFQEGALFNSLTVGGNLALPLETWT
                     DLPRDAIAALGRAKLRMVGLGEISKLVPAQLSGGMKKRVALARSLALDPALVFLDEPS
                     SGLDPVTSAEIDLLIGRLARAFGLTVILVTHELGSIHAIADRCILLDRESKSILATGT
                     PSELAANPHPRVRHFFGRIAEDA"
     gene            complement(5437..>6027)
                     /locus_tag="VFV99_29480"
     CDS             complement(5437..>6027)
                     /locus_tag="VFV99_29480"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5063559.1"
                     /translation="GGFVSALHAMPELKARGANPYVADLVGIAITRELAPLMTAIIMA
                     GRSGAAYAAELGTMHVSDEIDALETMGISPIPYLVIPRIVALALVAPLLVLLADVAGV
                     LGGLTVGVYYLDLTVQGFFTEMQTMVVTSDIWTGLIKSVAFAVAIAIVGCQEGLAARG
                     AAAGVGRRTTATVVVSLFTVVVIDSLLTIFFRAVGV"
BASE COUNT         1160 a         1909 c         1945 g         1013 t
ORIGIN      
        1 aatgtacgcg accgaatctt cgggaacgtt gtagccggct gcccaaagca accggttcgt
       61 gatgacgtgg cctccgcttt cgagctccgg gacgcctttc ttgtcgaact tgatcaggta
      121 gcgctcgccg cgcgagtcga cgatgaagac gccttgcgac acaccttcgc gcttgctgcc
      181 gaccacggtc cacggcacgt aggcctcggg tccctcgatc gtggccggcc cttgacgcac
      241 ctcatcaggc gtcagctcgc gcacaccgat gcgattcgtg aaccacgtcg agtccggaac
      301 ctcgtcgatg gaattcacgc cgagcgcacg gcggtgcggc ttcagctcca acggacgaac
      361 gatcggtctc accacggcac tgttgaattc cgacagatcg gtctcgggcc cgatcgactt
      421 tggaggtatg gcgacattgc ggcgatcgtc gacgacgcgg actgggttcg cgttcacgaa
      481 gcgcgccggc ggcagtggtg gcgacgccga acacgcgaca gcggacagag cgacggagac
      541 aaatcggagc ttcaccggcg tctcacgcgc ggctcgatcg ccgagaccgg gtcgagatag
      601 aggttgaacc acacgtcacc gtcgatcgag ctcgagacac tgagccgacc caccaagctc
      661 gtatgggtct ggacctcgag agaaaacccg tagccacagc gcatgccggc gagcgtgacg
      721 tcgtcccatg cggggtagac gtggccgaca tcggcgtaga cgctcgcgta cagccaacgc
      781 gcgagatccc attgatactc gagcgaaccg gccagcgcga tcttgtcgcg aaaccgatcg
      841 gtcgcgtagc cacgcagcca ttgccgacca ccgagcgacg gaacttcgtt gaacggtact
      901 tcgttgttct cggcggtgat cgcttcgcca tgaacacgcg ccgacagcac gcgcgggttt
      961 ccgccgatgg ggaagatctg ctgcaggtcg aagccgtagc ggaagaaatt ccggcccgtg
     1021 tcgagcgcgg tgattccgcc gtagaccgcc gcaagcgtgc cggccgacca cagcatttgg
     1081 ggctgccact gggacgtcgg accgcgcgag tcgtagcgaa gctgtccctc ggtgtagagc
     1141 gcgttgtagt gatcgaacgc gatcagcgag ctcggcatgt acacgtcctg gatcggcggc
     1201 cccttcgagc tgtcttcgcg atcgcgtcgc gcgaccgacg cgccgccatc gacatagaag
     1261 tcgcgcagga cttcgacatt gcccaccgcc gatgcgcgcg cgagcttgtc cttgtagtac
     1321 gagacgaacg cgaccggctc gtcgaacggg tcgaccggca tcacgggctc gaagtcgacc
     1381 tggtctgcgt tgccaaagcc gtagaatcgg ctctgcccgc gtacgtcgta ctcgacgtcg
     1441 aactgcgaat cgaggcgacc gtcgaggcga ttcgtcatct ggtacgtcgc cgaggtgcga
     1501 agtcgcgatg ccgtacccgc gccggcgaat agcttgatgt agtcgttcgg cgtcagctcg
     1561 gccacgaatt gcgcgccgag catcacgccc tgcgtgctct cgaagaagag cagcggatac
     1621 acgccgatcg tcatgtcgtc gttgaacaac caccgccgcg cggtcggcac gatcgcccat
     1681 ttgtcgttgg ccaggatcgc gccgcgcgcc ggcaggagca gcagcgcgac aatgccgcgc
     1741 gggatgtaga gcagcgtgcg ggcgaccttg cgtcccgcgg agtcaccgtc ggagggctcg
     1801 tcgatacggc cgctctcctc accgggtggt ggctggtact cgatcggcgc gacgggctgt
     1861 tccgtctctt gttctacggg cgtcggtggc tcgtcggcag gctgtgcctg ggctgagctg
     1921 accgacaaca agatggcgat cgagatcgca tgtttcatcc cagctctcct gcgatcagcg
     1981 gccggtgcgt caacgcttgg tgacgcctcg cgatcgctac gcagatcacc agccacacgg
     2041 cgacgagcgc aacattcacg atggcgagtg cgcgccggcc gaacgccagc gcgtgaatcg
     2101 caacagccac caatacggcc gacaacgtgt cgccgaaccg cacgaagaac gtgtcgatgg
     2161 ctgccttcgc tttgtacttc gccgcccgtg tgacgggcag aaacaacatt tggcgcacgg
     2221 tgttgtgcag cgagtaatcc gtgctgcctt cagtgagctt ggcggcgcgc acgacggcga
     2281 acgtgccgag catcgcgacg gaaccgtagg caccgaggac gatgaccgga agcacgaaca
     2341 acgcgcggcc gacgccgagc ttgcggatca cgcgcgagac gaacgccgcc tgcagcgcga
     2401 atgcgaccag attcacatgg aagaagaagt cgccgtagaa cgtcttgatc agctcgctgc
     2461 gctctgcggt ggcgtgtgga tcgggcgcga tctgcatcgc gcgctcgagc accgcgttcg
     2521 agagaatgta gtcgccagtc gtgttcacga gactcaggat cagcagcagg accgcgatca
     2581 acgcgaggta cggatcgcga agcaccagcg cgaagccacc ggcatcggca tcgacttctt
     2641 tgacgatgcg ctcatggcgc tgatggtcgt cgcagcggtc gacgatgttg atcagcatcg
     2701 cgtaggcgaa cagcaggatc ggcgatagca gcatcaggtc tcgagtcgtc gcgatctgtg
     2761 cgaggcgggg accggcgatg gcgcccatcg agccgccaag tgcaatcatc gcgaacacgc
     2821 gcttgccctg ctcttccgaa tagaagtcgt tggcgaacga ccagaactgc gcgatcaaga
     2881 acacgctaac gacgccgagc cagacgtaga acgccagacc aatcgacacc ccagcgtgcg
     2941 cggcaacgta gaacccggcc agtgagatca cgacgaacgc gatcgagata ttcacgagcg
     3001 cgatccggcg gagccgattc gccagcatgt cgtacgcggg aaccagcacg acgagtagca
     3061 atgccatcgc gccgctcgcg tacgtcttga cctcgtcgcc ctgcaaccgg aatgtcgtgc
     3121 tcgcgagcac gattccttca cgcgcagtct tcagtacgta gaagctcgtc aggagtagaa
     3181 acagcgcgag tagcaggagc acggcgcacg cgtgctctcg ggcctgcacc gtaccgcgtg
     3241 gcagcacact ccttggtacg tggtgttgcc cgtctcgacg tcgcgcccgg cgcatcgatg
     3301 acacgcgact gcattggaca tgccgtgcgc ttagttcgcg gtgagagatt ctgaggcaat
     3361 ctgcaggctc gcatcatcca acgcttggcc gatcgcaacg acgaatgaat cgaaacttgg
     3421 acctgcggtg tgctcggttt cgaccgtgcc ttcacgtatg accacacggt tgtcgatcaa
     3481 cgtgtagcgc agaccgacgc gggcgagcgg tcgtgggtca cgaacttcct cgaacgtgac
     3541 aacctcgacg tcgagctgga gtgccttacc gccggtgacc agcacaccgc gttcggcgag
     3601 cacttgctcg agcgcacggc gtgcatacgc ttccggcgat tcggcccatc gccgagtttc
     3661 gtagaactcg atctgtgtcg gcgacacgcg atacgcaatc cgcgcgcgca agtgcgacgc
     3721 agcggacacg cgcccgagcc gaatcttcgt gtcggatggc agtgcgtagg cctgggtggt
     3781 cggcgagacg tcgggcgtgt agtagtggac gtcgagcggt ggtgacttcg atgtcaacgc
     3841 acatcccgcg agcgcgagca gcgcgagtcg gcgcacggtc atcgtcgcct ccccggccgg
     3901 taacgcccct tgatcaggat gtcgggctcg cgctctagag tgtcgaggaa gtcgcgcagc
     3961 tgacgcgctg catcgccgat gtcgcggatc gtgctttcga gctcgccggt cgcatccgtg
     4021 gtccgttggc cgagctcgtc gagcgtgcca ttgatcgtgc ccagcgtctt cgcgatcgac
     4081 ttgggtagat cggcccgcgc agctccacgc gcgacgtatc cgatcgcggt caccgtgtcg
     4141 cgtgtctcct ccagtagcga gcgcgcgtcg tacgtcacgt gatggactgc gtcgagtgcc
     4201 gcgatcgcgt gatcggcgat catcggcagc cggtgtgcga atgtcacgac atcgtcctcg
     4261 agagagccga gtagcgacat ctccgacgga atgtagcgag cgggaggttt gaacgtgagc
     4321 tccggtgccg gcggcgtatg ctcgtcggca aagtcgagat cgatcagctt cacaccggtg
     4381 attccgaaga tcaccagtcg agcgcgcagc gtgagtgcag acttgtcgag tcgcagttcg
     4441 gccgcgcgcg agcgatcgat cgccatgccg acatcgatca gccgtcgatc cggcgcgacg
     4501 ctgatcgact tcaccttccc gatgcgaaca ccgcggaact tgacgatcgc gccctcgtcg
     4561 agaccctgga ccgattcgtt gaagtatgtg tgatagacgt cggtcggctt cttgcgcaga
     4621 ccgagcgcga gcatcgcact gacgacagcg gccgccgcga gcaacgcgag caagccgagc
     4681 ttgacgtggc gcgacgtcga cttcatgcgt cctccgcgat gcgcccgaag aagtgccgca
     4741 cgcgaggatg tgggttggcc gcgagctcgc tcggcgtgcc ggtcgcgagg atgctcttgc
     4801 tctcgcggtc gagaaggatg cagcgatcgg cgatcgcgtg gatgctaccg agctcgtgtg
     4861 tgacgaggat gaccgtcagg ccaaatgcgc gagcgagccg acctatcaag aggtcgatct
     4921 cggcggatgt cacgggatcg agaccggaag aaggctcgtc gagaaacacg agcgcgggat
     4981 cgagtgccaa cgaacgcgcg agcgcgacgc gctttttcat tccgccggaa agctgcgccg
     5041 gtaccagctt cgagatctca ccgagaccaa ccatgcgcag cttcgcgcga cccagcgctg
     5101 cgatcgcatc acgtggcaga tcggtccacg tctcgagcgg cagggcgaga ttgccgccga
     5161 cggtaaggga gttgaacaac gcaccctctt ggaacagcac gccgaactcg ggagcctcgg
     5221 cgcggaatgc aggcggccgg ccgagcacga gtacttcacc ggcccgcggc cgttcgagcc
     5281 cgatcagcac gcgtagcagc gtcgacttac cgctcgcact gcggccgagg atcgcgaaga
     5341 tctcaccgcg ctcgacaacg aaacttgcgt gctctagcag aatctcgtcc tcgctccagc
     5401 cgatcgtcag atcgtgagct tcgatcatcg gctcgctcat acgcccaccg ctcggaaaaa
     5461 gatcgtgagc agcgagtcga tcacgacgac cgtgaacaga ctgacgacga ccgttgccgt
     5521 cgtgcgccga ccaacgccag cggctgcgcc gcgcgcggcg agaccttcct ggcagccaac
     5581 aatcgcgatc gcgaccgcga acgcgacact cttgatgagt ccggtccaga tgtcagacgt
     5641 cacgaccatc gtctgcatct cggtgaagaa gccttgcacc gtgaggtcga ggtagtagac
     5701 gccgacggtg agaccgccga gcacgcctgc cacgtcggca agtagcacga gcagtggcgc
     5761 gacaagcgcc aaagcgacga tacgtggaat caccagatac ggaatcggcg agatgcccat
     5821 cgtctcgagc gcatcgatct cgtcgctgac gtgcatcgtg ccgagctcgg cggcgtacgc
     5881 tgcacccgag cgaccggcca tgatgatcgc ggtcatcagc ggtgcgagct cgcgcgtgat
     5941 cgcgatgccg acgaggtcgg cgacataagg attcgcgcca cgagctttga gctccggcat
     6001 cgcgtgcagc gcgctcacga agccgcc
//
LOCUS       DASUFX010000729         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_672293,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000729 DASUFX010000000
VERSION     DASUFX010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672293"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2164
                     /gene="glgX"
                     /locus_tag="VFV99_29485"
     CDS             <1..2164
                     /gene="glgX"
                     /locus_tag="VFV99_29485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5063560.1"
                     /translation="TQPSPRATQQVDALRPTKRAKWPGKPYPLGAIFDGSGTNFAIYS
                     SVAERVQLCLFIEGAEESIDLDQGIGGVWHAYLPEIGPSTRYGFRVHGPWDPANGLRC
                     NPHKLLVDPYARALSEGLEWSPALRAYKDDDPFGEMSTEDSAPYTFRSVVAQPYFDWG
                     NDRRLEIPWNETVIYEAHVRGLTMRHPAVPPALRGTYAGVAHHAVIEHLKTLGVTALE
                     LLPIHAFVHDGFLLERGLRNYWGYNTIGFFAPHPEYASVRSANGAVAEFKSMVRELHR
                     AGIEVILDVVYNHTAEGNHMGPHLSMRGIDNTAYYRVVADQPGYYMDYTGTGNTLQMQ
                     HPHVLQLVLDSLRYWVEEMHVDGFRFDLAAALARGLHEVDRLGPFFDLIQQDPVVSRV
                     KLIAEPWDVGEGGYQVGNFPPLWSEWNGKYRDWVRDYWRGEPGSLPELGSRFTGSSDL
                     YQNSGRFPHASINFITAHDGFTLRDLVSYNEKHNEANQEDSGESHNRSWNCGVEGETD
                     DPAVNAMRRRQQRNFLTTLFLSQGVPMLCGGDEIGRTQHGNNNAYCQDNEISWFDWEH
                     EDHELLEFVRELSRLRRHHPVLRRRGWFQGHPIRPHAQGPALPDIAWLTPDGDEMADD
                     HWDSAPTASLQVFLNGAGITVPDDHGEPIVDDTFLVIFHAHHEDQAIKLPGKRWGAAW
                     TRVLDTERGFAADENERYVAESDVQVLARTLWVLRRVA"
     gene            2587..>3777
                     /locus_tag="VFV99_29490"
     CDS             2587..>3777
                     /locus_tag="VFV99_29490"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5063561.1"
                     /translation="MIEPRATYRLQLHAGFTFADAAAAVPYLAELGISHLYLSPILTA
                     AKGSTHGYDVLDPERISDELGGEEGFAKLVATAHAAGLGILLDIVPNHMSIAGKGNRW
                     WYDVLENGPSSYYAHFFDVDWTVGDDRVLLPVLGERYGRAIQNGLIRIVELAGGKFAV
                     RVHDLELPVAPRSLGTLVKRAADRCNHLELAYIGDALLALPSLHRRESEARRRRHRDK
                     IVLLGRLAALSEDLAVSVALADEVAAVNADPAALDALLEQQAYQLVHWSVSANQISYR
                     RFFDINTLVGLRNEEEDVFEASHRRVLELVAAGDIDGLRIDHVDGLRDPQGYLTRLHE
                     RAPGAWIIVEKILTADEQLPAGWPVDGTTGYDFLEKVTGLLVDPSGERVLTDTFERFT
                     DTVWD"
BASE COUNT          644 a         1265 c         1241 g          627 t
ORIGIN      
        1 tacccagcca tctcctcgcg ctacccagca ggtcgacgca ctgcggccga cgaagcgcgc
       61 caaatggccg ggcaagccgt atccactcgg cgcgatcttc gacggctcgg gtacgaactt
      121 cgcgatctac tcgagcgtcg cggagcgcgt gcagctgtgc ttgttcatcg agggcgccga
      181 ggagagcatc gatctcgatc agggcatcgg cggcgtgtgg cacgcatatc tgcccgagat
      241 cgggccgagc acgcgctatg gctttcgcgt ccacggtccg tgggatccag cgaacggcct
      301 gcgctgcaat ccgcacaagc tgctcgtcga tccatacgca cgggcgctca gcgaagggct
      361 ggaatggtcg ccggcgctgc gtgcgtacaa ggacgacgat ccgttcggcg agatgtcgac
      421 cgaggacagc gcgccgtaca cgtttcgctc cgtcgtcgcg cagccgtact tcgactgggg
      481 caacgatcgc cggctcgaga ttccatggaa cgagaccgtg atctacgagg cgcacgttcg
      541 cggcctcacg atgcgtcacc ctgccgtgcc accggcgctg cgcgggacat atgcgggcgt
      601 cgcgcaccac gcggtgatcg agcacctcaa gacgctcggc gtcaccgcac tcgagctgtt
      661 gccgatccac gcgttcgtcc atgacggctt cttgctcgaa cgcgggctgc gcaattactg
      721 gggctacaac acgatcggat tcttcgcgcc gcatcccgag tacgcgagcg tgcgcagcgc
      781 caacggcgcc gtcgccgagt tcaagagcat ggtgcgcgag ctccatcgcg cgggcatcga
      841 agtgatcctc gacgtggtct acaaccacac cgccgagggc aaccacatgg gcccgcatct
      901 ctcgatgcgc ggcatcgaca acaccgcgta ctaccgcgtc gtcgccgatc agcccggcta
      961 ctacatggac tacaccggca cgggcaacac gctgcagatg cagcacccgc acgtgctgca
     1021 gctcgtgctc gattcgctgc gctactgggt cgaggagatg cacgtcgacg gcttccgctt
     1081 cgatctcgcg gcggcgcttg cccgcggtct gcacgaggtc gatcgcctcg gtccgttctt
     1141 cgatctgatc cagcaggatc cggtcgtgtc gcgcgtgaag ctcatcgccg agccatggga
     1201 cgtcggtgag ggcggctatc aggtcggcaa ctttccgccg ctgtggtcgg agtggaacgg
     1261 caagtatcgc gattgggtac gcgactactg gcgcggcgag cccggatcgt tgcccgagct
     1321 cggctctcgg ttcaccggca gctcggacct ctaccagaac agcggccgct tcccgcacgc
     1381 gagcatcaac ttcatcaccg cgcacgacgg cttcacgctc cgcgatctcg tctcgtacaa
     1441 cgagaaacac aacgaggcga accaggaaga cagcggcgag tcgcacaacc gctcgtggaa
     1501 ctgtggcgtc gaaggcgaga ccgatgatcc cgcggtcaac gcgatgcgac gccgccagca
     1561 gcggaacttc ctgacgacgc tgttcctctc gcaaggcgtg ccgatgttgt gcggcggcga
     1621 cgagatcggt cgcacgcagc acggcaacaa caacgcgtac tgccaggaca acgagatctc
     1681 gtggttcgac tgggagcacg aagaccacga gctcctcgag ttcgtgcgcg agctgtcgcg
     1741 gctgcgccgc catcatccgg tgctgcgccg gcgcggttgg ttccagggcc acccgattcg
     1801 cccgcacgcg cagggcccgg cgttgccgga catcgcgtgg ctcacgccgg atggcgacga
     1861 gatggcggac gaccactggg attcggcgcc gaccgcgtcg ctgcaggtgt tcttgaacgg
     1921 tgccggtatc accgtgcctg acgatcacgg cgagcctatt gtcgacgaca cgttcctcgt
     1981 gatcttccac gcgcaccacg aggaccaggc gatcaagctg ccgggaaagc gctggggcgc
     2041 cgcgtggacg cgcgtgctcg acaccgagcg cgggttcgct gccgacgaga acgaacgtta
     2101 cgtcgccgag agcgatgtcc aggtgctcgc gcgaacgctg tgggtgctgc gcagggtggc
     2161 gtagatgagc gagccggttg gttctgttcc gggcgtgcac gtgtcacgtg cacgtgcacg
     2221 tgcacggcgt ccggctgctg gcacgcttcc cagggagtcg tcccctcagc gatgcaacct
     2281 cgcgagtagg ccgcggcgat cgtcgtcgac gaaggcgtgc gccgagctcc gagccggcga
     2341 tttccgccca gccgacgaag tctccgcgga cgagcacgag cgtccggctg ctggcaggct
     2401 tcccagcgag tcgtcccctc acctcgcgat caggccgcgg cgatcgtcgt cgacgaaggc
     2461 ttgcgccgag ctccgagccg gcgatttccg cccagccgac gaagtcaccg cggacgagca
     2521 cgagcacgtg cacgtgcacg tggcacgtgt acgtgtacga gcagaagctg gcgctcgggg
     2581 caccaaatga tcgagccgcg cgcgacgtat cggctgcagc tgcacgccgg attcacgttt
     2641 gccgacgcgg cagcggcagt gccatatctc gccgagctcg gcatcagcca tctctatctg
     2701 tcaccgatcc tgaccgccgc gaagggttcg acgcatggct acgacgtcct cgatccagag
     2761 cggatcagcg acgagctcgg cggtgaggag ggctttgcga agctcgtcgc cacggcgcat
     2821 gcagcgggcc tcggcatctt gctcgacatc gtgccgaatc acatgtcgat cgccggcaag
     2881 ggcaaccgct ggtggtacga cgtgctcgag aacggcccgt cgagctacta cgcgcacttc
     2941 ttcgatgtcg actggacggt cggcgatgat cgcgtgctgc tgccggtgct cggcgaacgc
     3001 tacgggcgtg cgatccagaa cggcctgatc cgcatcgtcg agctcgccgg cggcaagttc
     3061 gccgtgcgcg tacacgatct cgaattgccg gtcgcaccgc gctcgctcgg tacgctcgtc
     3121 aagcgcgcgg cggatcggtg caatcatctc gagctcgcgt atatcggcga cgcgctactc
     3181 gcgctgcctt cgctgcatcg ccgcgagagc gaggcacgcc gccgccgcca tcgcgacaag
     3241 atcgtgctac tcggccggct cgccgcactg agcgaagacc tcgcggtgtc cgtcgcgctc
     3301 gccgacgaag tcgcggcggt caatgccgat ccggccgcgc tcgacgcgct gctcgaacag
     3361 caggcgtatc agctcgtgca ctggagcgtg tcggcaaatc agatctcgta ccgtcggttc
     3421 ttcgatatca acacgctggt cgggcttcgc aacgaggaag aggatgtctt cgaggcgagt
     3481 catcgccgcg tgctcgagct cgtggctgcc ggtgacatcg atggtctccg catcgaccac
     3541 gtcgacggcc tgcgcgatcc gcagggctac ctgacgcgac tgcacgagcg cgcgccgggt
     3601 gcgtggatca tcgtcgagaa gatcctgacc gccgacgagc agctgcctgc aggctggccg
     3661 gtcgacggca cgaccggcta cgactttctc gagaaggtca ccggcctgct cgtcgatcca
     3721 agcggcgagc gggtgctcac cgatacgttc gagcggttca ccgacacggt ctgggac
//
LOCUS       DASUFX010000730         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_811721,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000730 DASUFX010000000
VERSION     DASUFX010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811721"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFV99_29495"
     CDS             <1..1184
                     /locus_tag="VFV99_29495"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5063562.1"
                     /translation="TSSPTQGRAPTPSDLPQYGRAPTGHPSTGRVPTQGGTNDQGPEA
                     PRTITQSSGVPREAGDRGSSGANAASATGTFRAQRDRSPSDGVRRPGSQTNSPLSSRT
                     RTGSSGLHTRPSSDSIARPRTSSSSQMAALNPPTQRPGGQASIRRKQSTAATMEIEAL
                     IQKKIPMLDQGVDYFTLFGLQIGASPEDVRSTYLMLARKLHPDRLAAIGIEDDERHAQ
                     RLMACINEAFAVLNDPIRRAEYMSILDRGGEAAVRAENQQADELAMKVMRAEEAFRQG
                     EMCMRREQIAQAIVHFKEAVELQPAEPEYQALLAWAHFAHATDKNDVATLTRKALMKA
                     AEMNLDSPTARFYLGRVERMLGREKEALYWFYEVLKLKPNHSEARSEARILEQRIKAK
                     R"
     gene            complement(1185..>2561)
                     /locus_tag="VFV99_29500"
     CDS             complement(1185..>2561)
                     /locus_tag="VFV99_29500"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5063563.1"
                     /translation="SREMIVDDATLDLPVVELTALLDNGQLTSVALTEAYLARIAKRA
                     PALNCFITVTPERARADAAKADARRAAGERGPVLGIPYGLKDLIDTAGIATTWGTRSM
                     LERVPKTDADVAARLAAAGGVLLGKLHCTELANSFGVENQEDAHNGACRNPWSLDRWA
                     GGSSGGSGAAVAAGLCGFAIGSETLGSIDCPSAFCGVVGLRPTYDLVSRAGVMTAAYT
                     LDKLGPLTRDARDARAVLPLIATQPIEAKQRRLRVGIADVIDPKLSDPALAPLFDKAR
                     SILREIADVQPVTLPEIPTMPATLTILLAEIRAAFDDFIRAGHIHALYDRQSWATKER
                     EYLALDLRAEDYIKATRVRTQAQQVYRTLFDRFDVLLTPGRPEVARKLVGYKGAESDA
                     PNSYTTLLVTGNLIGAPAITLPIGFSAESLPLSMHAIGSPYDDDAVLALAELYQAHTD
                     FHRKRPPS"
BASE COUNT          463 a          841 c          868 g          389 t
ORIGIN      
        1 cgaccagcag cccgacacag ggtcgcgcgc cgacgccgag cgatctgcca cagtatggac
       61 gagcgccgac gggacatccg tcgacggggc gcgtgccgac gcagggtggg acgaacgacc
      121 aagggcccga ggcgccgcgc acgatcacgc aatcgagtgg cgtgccgcgc gaggcgggtg
      181 atcgcggctc gtcgggggcg aacgccgcct cggcgaccgg cacgttccgc gcgcaacgcg
      241 atcgatcgcc atcagacggc gttcgccgac ctggttcgca gaccaactca ccgctgtcat
      301 cgcgcacgcg aaccggcagc agcggcctgc atactcgtcc atcgagcgac agcatcgcgc
      361 gaccgcgcac gagctcgtcc tcgcagatgg ccgcgctcaa cccgccgacg cagcgccccg
      421 gcggccaggc gtcgattcgc cgcaagcaga gcacggccgc gacgatggaa atcgaggcgc
      481 tgatccagaa gaagatcccg atgctcgatc agggcgtcga ttacttcacg ttgttcggct
      541 tgcagatcgg tgcgtcgccc gaggacgtgc gcagcaccta cttgatgctg gcgcgcaagc
      601 tgcatcccga tcgactcgca gcaatcggca tcgaggacga cgagcgacat gctcagcgcc
      661 tgatggcatg catcaacgaa gcgttcgcgg tgctcaacga tccgattcgc cgcgccgagt
      721 acatgtcgat cctcgatcgc ggtggtgaag cagcggtacg tgccgagaat cagcaagctg
      781 acgagcttgc gatgaaggtg atgcgcgccg aggaggcgtt ccgccagggc gagatgtgca
      841 tgcggcgcga gcagattgcg caagcgatcg tgcatttcaa agaggctgtc gagctgcagc
      901 cggccgagcc cgagtaccaa gcgctgctcg cgtgggcgca cttcgcgcac gcgacggata
      961 agaacgacgt tgcgaccctg acgcgcaagg cgctcatgaa ggctgccgaa atgaacctcg
     1021 actcgccgac cgcgcggttc tacctcggtc gcgtcgagcg catgctcggc cgcgagaagg
     1081 aagcgctgta ctggttctac gaggtactca agctcaagcc gaatcactcc gaagcgcgga
     1141 gtgaagcgcg catcctcgag caacggatca aagcaaaacg ctagctagct cggcgggcgc
     1201 ttgcggtgga aatccgtgtg cgcctgatac agctcggcga gtgcgagcac cgcgtcgtcg
     1261 tcatacggcg agccgatcgc gtgcatggag agcggcaagc tctccgcgct gaaaccgatc
     1321 ggcagcgtga tcgcaggcgc gccgatcaga ttgccggtca cgagcagcgt cgtgtagctg
     1381 ttcggtgcgt cggattcggc acccttgtag ccgacgagct tgcgcgcgac ttcggggcga
     1441 cccggggtga gcagcacgtc gaagcgatcg aacagcgtgc gatacacctg ctgggcttgc
     1501 gtgcggacgc gcgtggcctt gatgtagtcc tccgcacgca agtcgagcgc cagatactcg
     1561 cgttccttcg tcgcccacga ttggcggtcg tacagcgcat ggatgtggcc cgcacgaatg
     1621 aaatcgtcga aggcggcgcg gatctcggca agcaagatcg tgagcgtcgc cggcatcgtg
     1681 gggatctcgg gcagcgtcac cggctggacg tcggcgatct cgcgcaagat cgagcgcgcc
     1741 ttatcgaaga gcggcgcgag cgcaggatcg ctcagcttcg gatcgatcac gtctgcgatc
     1801 ccgacgcgca gtcggcgctg cttggcctcg atcggctgcg tcgcgatcag cggcaacacg
     1861 gcgcgggcat cgcgtgcgtc gcgcgtcagc ggcccgagct tgtcgagcgt gtatgccgcg
     1921 gtcatcacgc ctgcgcggct gacgagatca tacgtcgggc gcaagccgac gacgccgcag
     1981 aacgccgacg ggcagtcgat cgagcccagc gtttcactgc cgatcgcaaa accgcacaga
     2041 ccggcagcca ctgccgcgcc ggaaccgccc gacgagccac cagcccagcg gtcgaggctc
     2101 cacggattgc gacatgcacc attgtgcgcg tcttcttggt tctccacgcc gaacgagttc
     2161 gcgagctcgg tgcagtggag cttgccgagc agcacaccgc cggcggctgc gagccgcgcc
     2221 gcgacgtcgg catcggtttt cggcacgcgc tccagcatcg accgcgttcc ccacgtcgtc
     2281 gcgatgccgg cggtgtcgat cagatccttc aggccgtacg gaatgccgag cacggggccg
     2341 cgctcgccgg cggcgcgtcg cgcatccgcc ttcgccgcgt cagcacgcgc gcgctcgggt
     2401 gtcaccgtga tgaagcagtt gagcgcggga gcgcgcttcg cgatccgcgc gagatacgcc
     2461 tcggtcaatg cgaccgacgt cagctggccg ttatcgagca gtgcggtgag ctcgacgacc
     2521 ggaaggtcga gcgtcgcgtc gtcgacgatc atttcacggc t
//
LOCUS       DASUFX010000731         7473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1367436, whole genome shotgun sequence.
ACCESSION   DASUFX010000731 DASUFX010000000
VERSION     DASUFX010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367436"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFV99_29505"
     CDS             complement(<1..657)
                     /locus_tag="VFV99_29505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063564.1"
                     /translation="MPLRHVKSDSIITSAPKKAKQAVLPEARAIQSRNASQSTIKPAK
                     QQGAKQQPVDAPPVAAQPPQAIPPFMPFEGGASFDPLAIPLPPELAPAVYGWLRRLAL
                     QADLAGADRLLRDALADLTSALQVLIIYSSPDGLHSIGANDEMPKDMQPVLAVAKSRR
                     ALVGTHSALIPIATSTDTIAVIQLVRNARQPAFNMVDHVTMAAIARESASIMHHLVVE
                     H"
     gene            943..2574
                     /locus_tag="VFV99_29510"
     CDS             943..2574
                     /locus_tag="VFV99_29510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5063565.1"
                     /translation="MRANITVALFALLIGSTVDARPRPRIAQEQNDPDQKDPDKDDGD
                     GPEAQAVLPLKLDDLIEVAIRLAPDATRAKVDRVAAKNLAEGERRQQAWIVSSNGQYQ
                     RNALAEDVEAPPFSIVSQEKISASLGLGRNLPTGGNVSVEAGVARESDEYLIVTRLRD
                     TANAQAPAGTDPNGNPYDYLFRNTASLRATLRQPIARGFGPKVALAPITKADLNASAE
                     TVKAQLATEELVRDLVVGYWDLALKSFEVDVRAQALDFAQKQEQLTHEQIRAGSAPSN
                     AANAVTYEIAMRQDALLRAQLELENMSLTLRQKSGLELGRRDVALRPAERFEIGEDEF
                     DVDEILERSHAANRKLATIQLQKKAADLDVTVARDQMKPQVDFTFSGAIMGNGDDAAS
                     AVSGVGGADSYEVMVGLQVQFELSGAAGKARDAAIAKRKRLDIDREDAVRQIDVAVVN
                     SVHMVTSARTRVALADKAIIVAEENVRAERASFMVGRTTNFNVMQRQTELIDARLRRG
                     QAVADYHKAVAQLQFLSGILLDQYRVNVKPRGERR"
     gene            2689..3702
                     /locus_tag="VFV99_29515"
     CDS             2689..3702
                     /locus_tag="VFV99_29515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063566.1"
                     /translation="MRTTIKALLCSVLAAACAPIGERGDGLDNADAGVDGAGNGVCDQ
                     YQTKTMDLTINGSAGFNNLPQSCWKLNGKLTLTGPAVTSLAKLGDLREVRDLVIDDTD
                     LTKIDTKSIVEVTGDITVRYNDKLTDIANLAAKQTAKSILVEYNGELTNLGGLAKAEV
                     VTGGTIIRNNAKLTTVDLGRATRLEGGLLVADNNLVTKLDAHSLQSVGDFTFRNNAAL
                     ADLGSLSALRFVHGTFTLDNNDNLVSLANSITSGMGTIDINVIISNNAKLSELGGLAR
                     TQYIGGSVQATSNSALTFCEVREIDCCVNSGQVLASGNQTTTCNQTGYSWCNQELGYC
                     PYM"
     gene            3735..5405
                     /locus_tag="VFV99_29520"
     CDS             3735..5405
                     /locus_tag="VFV99_29520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063567.1"
                     /translation="MRSQLISLAFIAAALQAADADAKPRRVVVLEFDGPRRLADSGRS
                     AVMGVLGDQYNIVATKNWDTARARAAGRGPQQWRQASKQAGVDAVIEGWVQAEGRHHV
                     LTVAVREAATGRELDTLSVRIKDDGVTPDASQKLGVQLDDLLSWIDGDINADPTPTLP
                     DVRTMRPLLGARDPDRDRRRIDDEDDDADDRSSDRRRSKTRRDDRVGRDDRRDRDGRD
                     DRNGRDGRDEVRDDRRDDRRDARDDRRDDRDQRDDRDDRHDDRDSDRRASDEPKLKKV
                     ATAEETDQDTRDLVKLFGPESKEAEIVTEGKTTHKPKPTPRFLIGAGPYVSARGMAFD
                     YDPTAKGSPPEYPGQTLKGFAASAAVYPMPREKEDGKISGVGFSFDIAHSVLSTFTAM
                     DDTGYGDYTMEHSAWQAGIHYRYPYEFLAIDLDGSYGNFTHRIVDLPESIQIPDTSYS
                     YLGIGAHLDLQVTEGTTVGFGAHYLYLLSAGDISDEDWYGSGTAWGATLDGDVLIPIS
                     GALYVKGAVQYQRVKVDFEGSGMLSDAWGVWDVVDSSISGSASLGVKF"
     gene            5628..5960
                     /locus_tag="VFV99_29525"
     CDS             5628..5960
                     /locus_tag="VFV99_29525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063568.1"
                     /translation="MSNTKDPFVSIEENQLETVSGGAARVASGGSSSDSNDQLLAMLT
                     SIGDSIKDLARNNNSGGGDQMMQMMMMMMMMGGMGGGGGGAVAAPAPQPPTYVEVSSS
                     GRGSKKGW"
     gene            6141..6437
                     /locus_tag="VFV99_29530"
     CDS             6141..6437
                     /locus_tag="VFV99_29530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063569.1"
                     /translation="MYELHAIEAINIDQLVTATDRLLLRCTRTIQRPGSRSTIDFSRE
                     AAAALAASEARRAATAHLEETVYMREPRRRRDVLSAAVLIASAWLGVICACLLA"
     gene            6547..6861
                     /locus_tag="VFV99_29535"
     CDS             6547..6861
                     /locus_tag="VFV99_29535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063570.1"
                     /translation="MKTKHTFSSIDREKLTAVSGGAARVAASAKSSDLDAKLQLMLSQ
                     IGDSIKAVGQNNQSSNDMLMPMMMMMMMGGGGGGGGAAPAQVAAPPPPPTGTYVKVNV
                     RR"
     gene            6866..7093
                     /locus_tag="VFV99_29540"
     CDS             6866..7093
                     /locus_tag="VFV99_29540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063571.1"
                     /translation="MADEFATIDLNALDAVTGGRYTAGPEQIRPELIQAIGELAKAVS
                     GVGQGLVAAKQQKDGQQLQMISQLMQMRGGR"
     gene            complement(7090..>7473)
                     /locus_tag="VFV99_29545"
     CDS             complement(7090..>7473)
                     /locus_tag="VFV99_29545"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX5063572.1"
                     /translation="CKRRFTTYEKLGAPALKVQKRDGTVEPFEGDKLFVSLQRVASHR
                     GVRDDDLRRVARDIEATLVDSGRKSVSWNDIVQLALDRLKNIDGVAANRFAANYVDET
                     GVVRFDAEAPPADARQLGLPLSDED"
BASE COUNT         1410 a         2431 c         2448 g         1184 t
ORIGIN      
        1 atgctcgacg acgaggtgat gcatgatgct cgcgctctcg cgagcgattg ccgccatcgt
       61 gacgtggtcg accatgttga acgccggctg acgcgcgttg cggaccagct ggatcaccgc
      121 gatcgtatcg gtcgacgtcg caatcggaat cagtgcgcta tgcgtaccga cgagcgcgcg
      181 ccgcgacttc gcgacggcga gcaccggttg catatccttg ggcatctcgt cgttggcgcc
      241 gatcgagtgc aggccatccg ggctcgagta gatgatgagc acctgcagcg cgctcgtcag
      301 atctgcgagg gcgtcgcgca gcaggcgatc ggcgcccgcg aggtctgctt gcagcgcgag
      361 gcggcggagc cagccgtaga cggcgggcgc gagctcgggc ggcagcggaa tcgcgagcgg
      421 gtcgaacgat gcgccgcctt cgaacggcat gaacggcgga atcgcctgcg gtggctgtgc
      481 agccaccggc ggtgcgtcta cgggctgctg cttcgcaccc tgctgctttg cgggcttgat
      541 cgtgctctgc gatgcgttgc gcgattggat cgcgcgcgct tcgggcagca cagcctgctt
      601 cgccttcttc ggcgccgacg tgatgatcga gtccgacttg acgtggcgca gtggcaccac
      661 ggcctcgtca ctcggcaggt atgtcggcgc gtcggcagca ggaccccatc cgcgaggatg
      721 agtcgaggcc gagaccgaat ggtcgatgcg catggcgagc cttagagcaa ccgccttgcc
      781 acgcgtaact gggcgaagcg tggacaaaaa ccgatcgaat cccgggggtg gggatcgcga
      841 ttcgtaaccc gaggtaggcg agtccccggg tcgccgatac gctgtcaacg cgaagactca
      901 atgagatggc gatcggcatg gcagctgctc tagccggtgc ccatgcgcgc caacatcacc
      961 gttgcactat tcgcgcttct gatcggatcc actgtcgatg cgcgtccgcg gccacgcatc
     1021 gcgcaggagc agaacgatcc tgatcaaaaa gatccagaca aagacgatgg cgatggtccg
     1081 gaagcgcaag cggtgcttcc cctcaagctc gacgatctga tcgaagtcgc gatccgcctc
     1141 gctcccgatg cgacgcgcgc caaggtcgat cgcgtcgccg cgaagaacct cgcggaaggt
     1201 gagcgccgcc agcaggcatg gatcgtttcg tcgaacggcc agtaccagcg caacgcgctt
     1261 gccgaagatg tcgaggcgcc gccgttctcg atcgtgtcgc aagagaagat cagcgcctcc
     1321 ctcgggctgg gccgtaacct gccgaccggc ggcaatgttt ccgtcgaggc gggtgtcgcg
     1381 cgcgagagcg acgagtacct gatcgtcact cgcttgcgcg acaccgcgaa tgcgcaagcg
     1441 cccgcgggca cggacccgaa cggcaatccg tacgactact tgtttcgcaa caccgcttcg
     1501 ctgcgcgcga cgctccgtca gccgatcgcg cgcggcttcg gccccaaggt cgcgctcgcg
     1561 ccgatcacga aggcagacct caacgcgagc gcagagacgg tgaaggcaca gctcgcgacc
     1621 gaggagctcg tgcgcgatct cgtcgttggc tactgggacc tcgcactcaa gtcgttcgag
     1681 gtcgatgttc gcgcgcaagc actcgatttc gcgcagaagc aggagcagct gacgcacgag
     1741 cagatcaggg cgggctcggc gccgtcgaac gcggcgaacg ccgtcaccta cgagatcgcg
     1801 atgcgccaag acgcgctgct ccgcgcgcag ctcgagctcg agaacatgtc cttgaccctc
     1861 cgccagaaat cgggcctcga gctcggtcgc cgcgatgtcg cgttgcgccc cgccgaacgt
     1921 ttcgagatcg gtgaggacga gttcgacgtc gacgagatcc tcgagcgcag tcacgctgcg
     1981 aaccgcaagc tcgcgacgat ccagttgcag aagaaggccg cggacctcga cgtcaccgtc
     2041 gcgcgcgacc agatgaagcc gcaggtcgac ttcacgttct cgggcgcgat catgggtaac
     2101 ggcgatgacg ccgccagtgc ggtgagcggg gtcggcggtg ccgatagcta cgaggtgatg
     2161 gtcgggttgc aggtgcagtt cgagctgtcg ggggcggcgg gcaaggcacg tgacgccgcg
     2221 atcgcgaagc gcaaacgttt ggatatcgac cgcgaagacg ccgtgcggca gatcgatgtc
     2281 gccgtcgtca actccgtcca catggtgacg agtgcccgga cccgcgtcgc gctggcggac
     2341 aaggcgatca tcgtcgccga ggagaacgtg cgcgccgagc gcgcgagctt catggttggc
     2401 cgcacgacga atttcaacgt catgcagcgg cagaccgagc tcatcgacgc acggctacgc
     2461 cgcggccagg cggtcgccga ctatcacaaa gccgtcgcac agctgcagtt cctcagcggc
     2521 atcttgctcg accagtaccg cgtgaacgtg aaaccgcgtg gggagcggag gtgagccatg
     2581 catccctcgg ttgacaaggc gaacgcgtcc cccacctcga cgaaaagccc aagcatcgag
     2641 atcaattgcg cgtgcgaacc ggcacgcgcg ttgctgaagg agacaaccat gcgcaccacc
     2701 atcaaggctt tgctttgctc ggttctcgcc gcagcgtgcg cgccgatcgg cgagcgcggt
     2761 gacggcctgg ataacgccga tgccggcgtc gacggcgccg ggaacggcgt ctgcgaccag
     2821 taccagacca agacgatgga cctcaccatc aacggcagcg ccggcttcaa caacctgccg
     2881 cagagctgct ggaagctgaa cggcaagctc acgctcaccg gcccggcggt gacgagcctc
     2941 gccaagctcg gcgacctgcg cgaagtccgc gacctcgtca tcgacgatac agacctgacg
     3001 aagatcgaca ccaaatcgat cgtcgaggtc accggtgaca tcacggttcg ctacaacgac
     3061 aagctgaccg acatcgcgaa cctcgccgcg aagcagaccg cgaagtcgat tctcgtcgag
     3121 tacaacggcg agctcaccaa cctcggtggc ctggcgaagg ccgaggtggt caccggcggc
     3181 acgatcattc gcaacaacgc gaagctcacg acggtcgacc tcggccgcgc gacgcgcctc
     3241 gaaggcggct tgctcgtcgc cgacaacaac ctggttacga agctcgacgc gcactcgctc
     3301 cagtcggtcg gtgacttcac gttccgcaac aacgctgcgc tcgccgacct cggctcgctg
     3361 tcggcgctgc gcttcgtgca cggcacgttc acgctcgaca acaacgacaa cctcgtttcg
     3421 ctcgcgaatt cgatcacgag cggcatggga acgatcgaca tcaacgtgat catcagcaac
     3481 aacgcaaagc tcagcgagct cggcggtctt gcccgcacgc agtacatcgg cggctcggtc
     3541 caggcgacca gcaacagcgc gctgacgttc tgcgaggtcc gcgagatcga ttgctgcgtg
     3601 aactcgggcc aggtgctcgc gtcgggaaac cagaccacga cgtgcaacca gaccggttac
     3661 tcgtggtgca accaagagct tggttactgt ccgtatatgt gatgagggct cttcttccgt
     3721 tcaggagaat caccatgcgc tcgcaactca tctctctcgc attcatcgcc gccgcacttc
     3781 aggcggccga tgccgacgcc aaaccgcgcc gcgtcgtcgt actcgagttc gacggaccgc
     3841 gccgactcgc tgatagcgga cgctcggcgg tgatgggcgt gctcggcgat cagtacaaca
     3901 tcgtcgccac gaagaactgg gacacggcgc gtgcgcgcgc cgccggtcgt gggccgcagc
     3961 agtggcggca ggcctccaag caagcgggcg tcgatgcggt gatcgaaggc tgggtgcaag
     4021 ccgaagggcg ccaccacgtg ctcacggtcg ccgtgcgcga agcggcgacg ggccgcgagc
     4081 tcgacacgct gagcgtgcgc atcaaggacg acggcgtgac gccggacgcg agccagaagc
     4141 tcggcgtcca gctcgacgac ttgctgagct ggatcgacgg cgacatcaac gccgacccga
     4201 cgcccacgct accggacgtg cgcacgatgc ggccgctgct cggtgctcgc gatccagatc
     4261 gcgatcgccg ccgcatcgac gacgaagacg acgacgccga cgaccgcagc tcggaccgcc
     4321 ggcgcagcaa gacgcgccgt gacgaccgcg tgggccgcga cgatcgccgc gaccgcgatg
     4381 gtcgtgatga tcgcaatggt cgcgatggtc gcgatgaagt gcgcgacgac cgccgcgatg
     4441 atcgccggga tgcgcgtgat gatcgccgcg acgaccgtga tcaacgcgac gaccgcgacg
     4501 atcgtcatga cgatcgtgac agcgaccgcc gcgcgtccga tgagccgaag ctcaagaagg
     4561 tggcgacggc cgaggagacc gatcaggaca caagagatct cgtgaagctg ttcggccccg
     4621 agagcaagga agccgagatc gtcaccgagg gcaagacaac gcacaaaccc aaaccgacgc
     4681 cgcgcttcct catcggtgcg gggccgtacg tctcggcgcg tggcatggcg ttcgactacg
     4741 atccgaccgc gaagggtagt ccaccggagt atcccggtca gacgctgaag ggctttgcgg
     4801 cgagcgcggc ggtctacccg atgccgcgcg agaaagagga cggcaagatt tccggtgtcg
     4861 gcttctcgtt cgacatcgcg cactcggtgc tgtcgacgtt caccgcgatg gatgacaccg
     4921 gctacggcga ttacacgatg gagcactcgg cgtggcaggc cggcatccac tatcgctacc
     4981 cgtacgagtt cctcgcgatc gacctcgacg gcagctacgg caacttcacg catcgcatcg
     5041 tcgacctgcc cgagtcgatc cagatcccgg atacgtcgta ctcgtatctc ggcatcggcg
     5101 cgcacctcga cctgcaggtc accgagggca cgacggtcgg cttcggcgcg cactacctct
     5161 acttgctctc cgccggcgat atctccgacg aggattggta cggctcgggc acggcgtggg
     5221 gcgcgacgct cgatggcgac gtcctcattc cgatcagtgg cgcgctctac gtgaagggtg
     5281 cggttcagta tcaacgcgtg aaggtcgact tcgaaggctc cggcatgctc tccgacgcgt
     5341 ggggcgtatg ggatgtggtc gacagctcga tctcgggtag cgcgagcctc ggcgtgaagt
     5401 tctagcagcg aattctcgac gagaaatgcg gtcggcgaca cgccggccgt tttcgttttc
     5461 ggcggcgaga aatcgctccc agctcgctgt ctcccgcgag agacgatctg cggagtgatc
     5521 acccggagac tcacttcgta acctcctggg atcgcatttc gggagcactg gcacggccga
     5581 tgctctagct gaggttgcgc aaccaaccat ccaccaaggg aacaaccatg tcgaacacca
     5641 aggatccgtt cgtctcgatc gaagagaacc agctcgagac cgtctcgggc ggcgccgcgc
     5701 gcgtcgcctc gggcggcagc tcgagcgaca gcaacgacca gttgctcgcg atgctcacct
     5761 ccatcggtga ctccatcaag gacctcgccc gcaacaacaa cagcggcggc ggcgatcaaa
     5821 tgatgcagat gatgatgatg atgatgatga tgggcggcat gggtggaggc ggcggcggcg
     5881 cagttgcggc gcccgctccg caaccaccca cgtacgtcga agtgtcgtcg tcggggcgtg
     5941 gctccaaaaa gggctggtag ccgttacgcc acccgaacgg gtccagcggt ccgaagcagg
     6001 tgcttcgggc cgtttcgcgt tttcggacgc cgcctcgatg acgcgcgacg actaccacgg
     6061 acggcagcca tttccgacga gcacgtgcga gtccgcatgg ttagtcgcgg catgtcagct
     6121 gcacttcgaa ccggtcagcc atgtacgagc tccacgctat tgaagccatc aacatcgacc
     6181 aactcgtcac cgcgacggat cgcttgctgc tgcgctgtac gcgcacgatc cagcggcccg
     6241 gctcgcgaag cacgatcgac ttctcgcgcg aggcagcggc ggcgctggca gcgtcggagg
     6301 cgcggcgggc agcgaccgcg catctcgagg agaccgtcta catgcgcgag cctcgccgcc
     6361 gccgcgacgt gctgagcgcc gcggtgctca tcgcctccgc gtggttgggc gtgatttgcg
     6421 cttgtctact cgcgtaggcg cgccccgcga gctccgcgcc gcgagctccg cgaaatcaca
     6481 cagcgccgtc gctggcacgc ccgatgctct tcacctcgtc accaacatcc accaaggaac
     6541 tgcgcaatga agaccaagca cacctttagc tcgatcgatc gcgagaagct caccgccgtc
     6601 agtggtgggg cggcgcgcgt cgcggcgtcg gcgaagagca gcgacctcga cgcgaagttg
     6661 cagctcatgc tgagccagat cggcgattcg atcaaagcgg tcggccagaa caaccagagc
     6721 agcaacgaca tgctgatgcc gatgatgatg atgatgatga tgggcggtgg cggtggtggt
     6781 ggtggtgcgg cgcccgcgca agtggcggct cctcctccgc cgccgaccgg cacgtacgtc
     6841 aaagtcaacg tgcgccggtg atgtcatggc cgacgaattc gcgacgatcg atctgaacgc
     6901 gctcgacgct gtcacgggcg gtcgctacac cgcgggcccg gagcagatcc ggccggagct
     6961 gatccaagcg atcggcgagc tcgcgaaagc cgtcagcggc gttggtcagg gcttggtcgc
     7021 agccaagcag cagaaagacg gccagcagct gcagatgatc agccagctga tgcagatgcg
     7081 cggcggccgc taatcttcgt cactgagcgg gagccccagc tgcctggcat cggcaggtgg
     7141 ggcttctgcg tcgaagcgca cgacgccggt ctcgtcgacg tagttcgcgg cgaagcgatt
     7201 tgcggcgact ccgtcgatgt tcttcaagcg atcgagcgcg agctgcacga tgtcattcca
     7261 cgacaccgac ttgcggccgc tatcgacgag cgttgcttcg atgtcgcgtg cgacgcggcg
     7321 cagatcgtcg tcacgcacac cacggtgcga cgcgacccgc tgcagcgaca cgaacagctt
     7381 gtcgccctcg aacggctcga cggtgccatc gcgcttctgc accttgagcg cgggcgcgcc
     7441 gagcttctcg tacgtcgtga agcggcgctt gca
//
LOCUS       DASUFX010000732         8315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1784928, whole genome shotgun sequence.
ACCESSION   DASUFX010000732 DASUFX010000000
VERSION     DASUFX010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784928"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFV99_29550"
     CDS             complement(<1..792)
                     /locus_tag="VFV99_29550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5063573.1"
                     /translation="MRTTKYLFNTMLALCLASPIACSFGGSTDESALSNKHGGSNSGD
                     CTLTQGYWKNHPDAWPVVSLQLGTVTYTKVELIAILKTPVKGNGLVQLAHQLIAAKLN
                     IAAGASEVDVKASIQAADALIGSLVCPPKGEGELATSATSSLTGKLDAFNSGAVGPGH
                     CGDNDEGDDDHCDGGGHCGDDDGDTDDDDDHGGCDQPVCGNGDVETGEACDDGNTTNS
                     DGCSSTCAIEVCVCGDGVKSATEACDDGNTTNGDGCSSTCQAEPGW"
     gene            complement(922..2271)
                     /locus_tag="VFV99_29555"
     CDS             complement(922..2271)
                     /locus_tag="VFV99_29555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063574.1"
                     /translation="MKRALVLFVLLCALPAQAHVGSPDVFFSGEAGAYHLDVTVRMPI
                     VIPGVAEIEIRARDADVSGMTVVPLRLTGPGSDLPPTPDRADPSRADRQFFTASLWLM
                     EHGAMQVRIAVDGARGKGELAVPINAVAQRTLGMNRPLALLLLGLMLLLSLSVISIAA
                     AAVREGSLEPGIEPSPRARRKSKIAIGVAGALVGVVLWFGNAWWSSEAKDYERMILKE
                     WRIAPTRDGCTLHLPSLPAVMADHGHEMHLFVVRTPGLDRLAHLHPTRADDQTFVQTL
                     PSLPAGHYALFADVVFGGGFPMTAVAEIDLPDLTCPAPTGDDSAWDGTPSQTIRFERP
                     PQLRAGVAQSLQFAVTSPDGAPATDVEPYMGMAGHAAVLRRDLSVFAHLHPNGSVAMP
                     ALMLARTPHEMFAAGRTLPPHVAFPYGFPKPGDYRVIVQIKRSGRVETAAFDVSVAP"
     gene            complement(2268..3371)
                     /locus_tag="VFV99_29560"
     CDS             complement(2268..3371)
                     /locus_tag="VFV99_29560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063575.1"
                     /translation="MKVPWYVWTALASVSCILIGGYWDISWHMTVGRDSFWTPAHIAI
                     QLGGIIAGTAGGYLIIATTLGRTQLRDVSVRVWGFRGPLGAFVAVWGAAAMVISAPFD
                     NWWHAAYGLDVKILSPPHAVLTLGILAVATGGVLLIVAPLNRAAARTQLLEALLLVVA
                     GEVLVLGMTSILEHTFRSNLHRGDAYRAMAIVGPLLTLSFGRMSSHRWATTLIAGFYM
                     AFMMGMVWLFPLFPAEPKLGPVYQPITHFIPLEFPMLVIAPAFAFDLVRPRIESWPRW
                     RRAIVFGPLFVAVLLASEWPFADFLMSEHARNWFFGTHYLPYMMHPDWYEASYEFMPD
                     GDLVGGIVQAVIAACLTSYLGLVLGDAMRRAKR"
     gene            complement(3426..5342)
                     /locus_tag="VFV99_29565"
     CDS             complement(3426..5342)
                     /locus_tag="VFV99_29565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063576.1"
                     /translation="MGKSTRFAVCALAVALSAPRDVAAADHQFPAGSLIIPMDLSYQA
                     TGMFQAYGLIYQLMRQGVHVHWVIDPNKTWHAAPCNTASDLCTWDCGVEGSGVKCTYP
                     TASPDFAATTKVVWSDAGVARDTVVGMHRYRGGPFVVDAAQHDAALAIIDAWNDQSKW
                     AANPWAMRTVFHVVTVHEATAAFTGNSAREMIAAPTIAVFADGNEDIATSYLRAAGIP
                     QSNATEFPSGKCGASNCGPGTINPDMLTEEAIAGDLGTCDAPNNDHQNGALWKSDGTP
                     AYCQIMSMHWGVNEREKVQCAGGNCPATQAQCAAGTKFTFNGHEVVAEVRAFLAYSTH
                     FFAECQAVNAYENLVPNPAWPYLDDPGRDGHFLTTTGTPPACPNGTCANANYACVTNG
                     CGAGMNCCLPTPMTWQNMPGYEVATQPASAEVKVLRPDVPYNQIDGAHGTVGGSEPAY
                     NLSQYLGTMYKNNRQVTLLTGMNGPGNQDLWMSGYLDGCGDIILKPGQQRHAGCEGKI
                     SYLAGHKYDTTVPLTSASSSMGTRLFLNALFEAQCTTGGVGPGGTDTDGDGVDDSTDP
                     FPDDPMKCGDSDSDGCDDCSSGTFDPSNDCTGGGDDNGSPSGCCETGSDSRQLLLAAL
                     VGIALLRPRRRRQR"
     gene            5812..6123
                     /locus_tag="VFV99_29570"
     CDS             5812..6123
                     /locus_tag="VFV99_29570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063577.1"
                     /translation="MYEAISDADLVHVVGGCVCQPQQPTDPNAGGTTGAGAPPAGPTQ
                     PSGAPAPGAGGQPSSCDQLLQAITQLLNLFGGMNGAGAPGTAPGTAPGMAPKQPTAGA
                     Q"
     gene            complement(6127..7701)
                     /gene="ggt"
                     /locus_tag="VFV99_29575"
     CDS             complement(6127..7701)
                     /gene="ggt"
                     /locus_tag="VFV99_29575"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5063578.1"
                     /translation="MSTTGMVATSQRAATKVGVDVLRAGGNAVDAAIAAAAALAVTEP
                     VSTGLGGDCFALYYDAATKKLHALNGSGRAPAGLTRERFAERVHDNPLDANAVTVPGA
                     CAGWLDLLAAHGTWKRDRVLAPAIELAEHGYAVGDITAHYWQLNADRLGKLQRDISEL
                     TIEGRAPHSGETFRNPGMAKTLRTISNGGKDAFYRGEIAAAIEAVVQGAGGALTQADL
                     AAHTSTWDEPISTTYRNVRVWECPPNGQGITALLALNILENIELAGLDPLGADRMHIL
                     IEAMRLAFADTRWYVADPHVTHVPVAELLAKDYAKARAALIDRTRATADVKRGSPVAS
                     SDTVYFCVVDGDGNACSFINSNYMGLGTGLVPKGFGFSLQNRGANFSSDPTHPNVVAP
                     GKRPYHTIIPGMLTNLDGTLVGPFGVQGGFMQPQGHVQVVTAMIDDGVAPQAALDRAR
                     FSIEPVDGAQAILLEQDVPAATVAELERRGHKVKGGITGYERSAFGRGQIIRRDAGGH
                     LTGGSDNRADGVVEST"
     gene            7887..>8315
                     /locus_tag="VFV99_29580"
     CDS             7887..>8315
                     /locus_tag="VFV99_29580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063579.1"
                     /translation="MSQWNATSTKVSAIFVAGVAGVVLVAYATSDSSKPPPPRPHHVR
                     SHRSCPPLEPLPSDGSEEGHFYELDHLAKQELASHHLDAAAALAAELLELAPKYEQNW
                     NYGNAVHGGHTVLGQVALARGDVAEARRQLLASGATPGSPQ"
BASE COUNT         1422 a         2820 c         2691 g         1382 t
ORIGIN      
        1 ccatccgggc tcggcctggc acgtcgacga gcagccatcg ccgttcgtcg tgttgccgtc
       61 gtcgcacgcc tcggtcgccg acttgacgcc atcgccgcag acgcagacct cgatcgcgca
      121 ggtcgacgag cagccatcgc tgttcgtcgt gttgccgtca tcgcacgcct cgccggtctc
      181 gacgtcgccg ttaccgcaca ccggctgatc gcagccacca tggtcatcat cgtcgtcggt
      241 atcaccgtcg tcgtcgccgc agtgaccacc accatcgcag tggtcgtcat cgccctcgtc
      301 gttgtcgccg cagtgaccgg ggccaaccgc gccgctgttg aacgcatcga gcttgccggt
      361 gagcgacgac gtcgcgctgg tcgcgagctc gccctcgccc ttcggcggac acacgaggct
      421 gccgatcagc gcgtcggcgg cctggatcga cgccttcacg tcgacctccg acgccccggc
      481 cgcgatgttg agcttcgctg cgatcaactg atgcgcgagc tgaacgaggc cgttgccttt
      541 gaccggcgtc ttcaagatcg cgatcagctc gaccttcgtg tacgtgaccg tgccgagctg
      601 cagcgacacg acgggccacg cgtcggggtg attcttccag tagccttgtg tcagcgtgca
      661 gtcgccactg ttgctgccgc cgtgcttgtt cgacaacgcg gactcgtcgg tgctaccgcc
      721 aaacgagcac gcgatcggcg acgcgagaca cagcgccagc atcgtgttga ataggtattt
      781 ggtagtgcgc attctgagct cctcgggttt tcggagtcct cagttgccag tcgcgtacct
      841 aacaattgac ctcgcatttg tatgcacttg cacattcact gcgtgcgccg acgagacagc
      901 gtgaacgcac ggtcgcgcgg actacggagc gacagacaca tcaaacgcag cggtctcgac
      961 tcgaccacta cgtttgatct gcacgatcac gcgatagtcg ccgggcttcg ggaacccata
     1021 cggaaacgcg acgtgcggcg gcaacgtgcg accggccgcg aacatctcgt gcggggtgcg
     1081 cgcgagcatg agcgcaggca tcgcgaccga tccgttcgga tgcagatgcg cgaacacgga
     1141 tagatcgcgg cgcaacaccg ctgcgtggcc ggccatcccc atgtacggct cgacgtccgt
     1201 cgcgggcgcg ccgtcgggac ttgtcaccgc gaactgcagc gactgcgcca cgccagcacg
     1261 cagctgcggc ggtcgctcga agcgaatcgt ttgcgatggc gtgccgtccc aagcggaatc
     1321 gtcgccagtc ggcgcggggc acgtgagatc gggaagatcg atctcggcga ccgcggtcat
     1381 tggaaagccg ccgccgaaca cgacgtcggc gaacaacgcg tagtgcccgg ccggcagcga
     1441 cggcaacgtc tggacgaacg tctgatcgtc cgcgcgcgtc ggatgcaggt gcgccagccg
     1501 atcgaggccc ggcgtgcgca cgacgaacaa gtgcatctcg tgcccgtgat cggccatcac
     1561 cgccggcagg ctcggcagat gcagcgtgca gccgtcgcgc gtcggcgcga tccgccactc
     1621 cttcaggatc atccgctcgt agtccttggc ctccgagctc caccatgcgt tgccgaacca
     1681 cagcacgacg ccgacgagcg cgccggcgac tccgatcgcg atcttgctct tgcgtcgcgc
     1741 gcgtggcgac ggttcgatgc cgggttcgag gctgccttcg cgcacggccg ccgcggcgat
     1801 gctgatcacc gacagcgaca acagcagcat caggccgagc agcagtagcg cgagcggccg
     1861 gttcatgccg agcgtgcgct gcgcgactgc attgatcggc accgcgagct cgcccttacc
     1921 gcgcgcgccg tcgacggcga tccgcacctg catcgcgccg tgctccatga gccacaggct
     1981 tgccgtgaag aactggcgat cggccctcga gggatcggcg cgatctggtg tcggcggcag
     2041 gtcggagccc ggccccgtga gccgcaaggg cacgacggtc attccgctga cgtcggcatc
     2101 gcgcgcgcgg atctcgatct cggcgacgcc ggggatcacg atcggcatgc gtacggtgac
     2161 atccaggtga tacgcgccgg cttcgccgct gaaaaacaca tcggggctgc cgacgtgagc
     2221 ctgtgccggc aacgcgcaca acagcacgaa caggacgagc gcgcgcttca tcgcttcgcc
     2281 ctccgcatcg catcgccgag cacgagcccg agatagctcg tgaggcaggc cgcgatcacc
     2341 gcttgcacga tgccaccgac gaggtcgccg tcgggcatga actcgtagct cgcctcgtac
     2401 cagtcgggat gcatcatgta cggcaggtag tgcgtgccga agaaccagtt gcgcgcgtgc
     2461 tcgctcatca ggaagtcggc gaacggccac tccgaggcga gcaggaccgc gacgaacagc
     2521 ggaccgaaga cgatcgcgcg acgccagcgc ggccagctct cgattcgcgg ccgcacgaga
     2581 tcgaatgcga acgccggcgc gatcacgagc atcggaaact cgagcggaat gaagtgcgtg
     2641 atcggctggt agaccggccc gagcttcggc tccgccggaa acagcgggaa cagccacacc
     2701 atccccatca tgaacgccat gtagaagccg gcgatcagtg tcgtcgccca gcgatgactc
     2761 gacatccggc cgaacgacag cgtgagcagc gggccgacga tcgccatcgc gcgatacgcg
     2821 tcaccgcgat ggaggttcga gcgaaacgtg tgctcgagga tcgacgtcat gccgagcacg
     2881 agcacctcgc ctgccaccac gagcagcagc gcttcgagca gctgcgtgcg cgcggcagcc
     2941 cggttgagcg gcgcgacaat caacagcacg ccaccggtcg cgaccgcgag gatcccgagt
     3001 gtgagcaccg cgtgcggcgg gctgaggatc ttcacgtcga gcccgtaggc cgcgtgccac
     3061 caattatcga acggcgccga gatcaccatc gcggccgcgc cccacaccgc aacaaatgcg
     3121 ccgagcggtc cgcggaatcc ccatacgcgc accgacacat cacgtagctg tgtgcgaccg
     3181 agcgtcgttg cgatgatcag atagccgccc gcggtacccg cgatgatgcc gccgagctgg
     3241 atcgcgatgt gcgcgggcgt ccagaacgag tcgcggccca cggtcatgtg ccacgagatg
     3301 tcccaatagc cgccgatcag gatgcagctc accgaggcga gcgcggtcca cacgtaccag
     3361 ggcaccttca cgcctacgtg ctagcgcgag gtaccgcggg gcggcaagaa caagatcgtt
     3421 accgcctagc gctggcggcg acggcgcggg cgcagcagcg cgatgcccac gagtgccgcg
     3481 agcaagagct ggcggctgtc gctaccggtc tcgcagcagc cgctcggcga gccgttgtcg
     3541 tcgccgccgc cggtgcagtc gttcgacgga tcgaacgtgc cgctcgagca gtcgtcgcag
     3601 ccgtcgctgt cgctgtcgcc gcacttcatc ggatcatcgg ggaacggatc cgtgctgtcg
     3661 tcgacgccgt cgccgtcggt gtccgtgccg ccggggccaa cgccgccggt cgtgcactgc
     3721 gcttcgaaca gcgcgttcag gaacaggcgc gtgcccatac tcgacgaggc cgaggtcagc
     3781 ggcaccgtcg tgtcgtactt gtggccggcg aggtagctga tcttgccctc gcaaccggcg
     3841 tgccgttgtt ggccgggctt caggatgatg tcgccgcagc cgtcgagata gccgctcatc
     3901 catagatcct gattgccggg accgttcatg ccggtgagca gcgtgacctg gcggttgttc
     3961 ttgtacatcg tgccgaggta ctggctcagg ttgtacgcgg gctcgctgcc accgacggtg
     4021 ccgtgcgcgc catcgatctg gttgtacggc acgtcggggc gcagcacctt gacctcggcg
     4081 gatgccggct gggtcgcaac ttcgtagccg ggcatgttct gccacgtcat gggcgtcggc
     4141 aggcagcagt tcataccagc gccgcagccg ttcgtgacgc aggcgtagtt cgcgttcgcg
     4201 cacgtgccgt tcggacacgc aggcggcgta ccggtcgtcg tcaggaagtg accgtcgcgg
     4261 cccgggtcgt cgagatacgg ccaggccgga ttcggcacca ggttctcgta cgcgttcacc
     4321 gcctggcact cggcgaagaa gtgcgtcgag tacgcgagga acgcgcggac ctcggcgacg
     4381 acctcgtgac cattgaacgt gaacttcgtg cccgctgcgc actgagcttg agtcgccggg
     4441 cagttgccac cggcgcactg caccttctcg cgttcgttga cgccccagtg catcgacatg
     4501 atctggcagt acgccggtgt gccatcggac ttccacagcg cgccgttctg gtggtcattg
     4561 ttcggcgcgt cgcacgtgcc gaggtcaccg gcaatcgctt cctcggtcag catgtccggg
     4621 ttgatggtgc ccggtccgca gttgctggcg ccgcattttc ccgacgggaa ctcggtggcg
     4681 tttgactgcg gaatgcccgc ggcgcgcaga tagctcgtgg cgatgtcttc attgccgtcg
     4741 gcaaacaccg cgatcgtcgg cgctgcgatc atctcgcgcg ccgagttgcc cgtgaatgcg
     4801 gcggtcgctt cgtgaacagt cacgacatgg aacaccgtgc gcatcgccca cgggttcgca
     4861 gcccacttcg actgatcgtt ccacgcgtcg atgatcgcga gcgccgcatc gtgctgagcg
     4921 gcgtcgacga caaacgggcc gccgcgatac cgatgcatgc cgaccacggt gtcgcgtgca
     4981 acaccggcgt cgctccacac gaccttcgtg gtcgcggcga aatcgggact cgcggtcgga
     5041 tacgtgcact tcacgccgga gccttcgacg ccgcagtccc acgtgcacag atcgctcgcc
     5101 gtattacatg gcgccgcatg ccacgtcttg ttcggatcga tcacccagtg cacgtgcacg
     5161 ccctggcgca tgagctgata gatgagcccg tacgcctgga acatgccggt cgcctggtac
     5221 gacagatcca tcgggatgat caggctgccg gctggaaact gatgatccgc agcggcaaca
     5281 tctcgcggcg cgctcagcgc aactgcgagc gcacatacag caaagcgagt cgatttaccc
     5341 atcgactcat agtgccacta gcgccggaaa aagttgccgc tgtgccgcgc gcacatgtac
     5401 gacacgcgcg atctcagtaa gtgccgtagt tgttcgcgaa gctcagcgtg gcaccaagac
     5461 gcccacagcg agctgctctg cttcaataag catgagtctc cgttgactga ctgacgacat
     5521 acatcgcaac tcgcgttccc gacgacattc gtctcctcga ggaatcacat tgctgcccat
     5581 ggtcggcggc gcactgcgag caagcctgcg aacaaacacc gtgcgtctcc tcgcggcgac
     5641 ggcgacatgc gccaccgtgc tggctagtcg cgactccagg gctagcggtg gcgccggtcg
     5701 gcgcgctgcg cggcggctgc gacttacgcg agaaacgtcg acggcgggat acgtctgatc
     5761 gctggcacct gcactgcagt ctgtcgcttc gccaccaaca ggagaacagc gatgtacgaa
     5821 gcgatctcgg atgcggatct agttcacgtc gtcggcggct gcgtatgcca accgcagcaa
     5881 cccaccgatc cgaacgcagg cggcacgacc ggcgcaggcg ctccgccggc gggtccgaca
     5941 cagccgagcg gcgcgccggc tccgggtgcc ggcgggcagc cgagcagctg cgatcagctg
     6001 ctgcaagcga tcacacagct gctcaacctg ttcggcggaa tgaacggtgc cggagctccg
     6061 ggtaccgctc cgggtaccgc tccgggtatg gctccgaaac agccgaccgc gggcgcacag
     6121 tagtcgttag gtcgactcga cgacgccgtc ggcgcggtta tcgctgccgc ccgtgagatg
     6181 accgccggcg tcgcgccgaa tgatctggcc gcggccgaac gccgatcgct cgtaaccggt
     6241 aatgccgccc ttcaccttgt gaccgcggcg ctcgagctcg gcaaccgtcg ccgccggcac
     6301 atcttgttcg agcaggatcg cctgcgcacc gtcgactggc tcgatcgaga agcgcgcgcg
     6361 atcgagcgca gcttgcggag cgacgccgtc gtcgatcatc gcggtgacga cctgtacgtg
     6421 cccttgcggc tgcatgaagc cgccctgcac gccgaatgga ccgacgagtg tgccgtcgag
     6481 gttcgtgagc atgcccggga tgatcgtgtg atatggccgc ttgccgggtg cgacgacgtt
     6541 cgggtgcgtc ggatccgagg agaagttcgc gccgcggttc tgcagcgaga agccaaagcc
     6601 cttcggcacg agaccggtac caaggcccat gtagttgctg ttgatgaacg agcacgcgtt
     6661 gccgtcgccg tcgacgacgc agaagtagac ggtgtcgctg ctcgcaaccg gcgagccgcg
     6721 cttgacgtcc gccgtagccc gggtgcgatc gatcaatgcg gcgcgcgcct tcgcgtagtc
     6781 cttcgcgagc agctcggcga cagggacgtg cgtgacgtgt ggatccgcga cgtaccagcg
     6841 cgtatcggca aacgcgagac gcatcgcttc gatcaagatg tgcatgcgat ctgcgccaag
     6901 tggatcgagg ccggcgagct cgatgttctc gaggatattg agcgcgagca gcgcggtgat
     6961 gccctgcccg ttcggcgggc actcccatac gcgcacgttg cgatacgtcg tcgagatcgg
     7021 ctcgtcccac gtcgacgtgt gcgcggccag atctgcctgc gtcagcgcgc cgccggcacc
     7081 ctgcaccact gcttcgatcg ctgccgcgat ctcaccgcga tagaacgcat ccttgccgcc
     7141 atttgagatc gtgcgcagcg tcttcgccat gcccgggttg cgaaacgtct cgccactgtg
     7201 cggcgcgcgg ccctcgatcg tcagctcgga gatgtcgcgc tgcagcttgc cgagtcggtc
     7261 ggcgttgagc tgccagtagt gcgcggtgat gtcaccgacc gcgtagccat gctccgcgag
     7321 ctcgatcgcc ggtgcgagca cgcgatcacg cttccacgtg ccgtgcgcgg cgagcaggtc
     7381 gagccaaccg gcacacgcgc caggcacggt cacggcgttc gcatcgagtg gattatcgtg
     7441 gacgcgttca gcgaaccgct cgcgcgtgag cccagccggt gcgcggcccg atccattgag
     7501 cgcgtgcagc ttcttcgtcg ccgcgtcgta gtagagggcg aagcaatcgc cgccgagccc
     7561 cgtcgagacc ggctcggtga ccgcgagtgc cgccgccgcc gcgatcgcgg cgtcgaccgc
     7621 attgccgccc gcacgcagca catcgacgcc gactttcgtc gcagctcgct gactggtcgc
     7681 gaccatgccg gttgtgctca cgctctctct cctcaattga cccgcatgac cgcacgcgag
     7741 tgcccccgcc gctccaccgg tcatggcaag gaactcacga cgcgagatgc cacgcatagc
     7801 taggacttat cgcagattcc tttgcgatac gctcggccca agcgatcacg agtgaaacgg
     7861 cggcccgatt ggtgtttcat gtactcgtga gccagtggaa cgctacatcg acgaaagtca
     7921 gcgcgatctt cgtcgcgggc gtcgccggag tcgtgctcgt cgcgtacgcg acgtccgatt
     7981 cgagcaagcc cccgccgccc cggccccacc acgtccgcag ccatcgtagc tgcccgccgc
     8041 tcgagccgct tccttccgac ggttcggagg aagggcactt ctacgagctt gaccatctcg
     8101 ccaagcaaga gctcgcgtcg catcatctcg atgccgccgc cgcactcgcg gcggagctac
     8161 tcgagctcgc accgaagtac gagcagaact ggaactacgg caacgcagtc catggcggcc
     8221 acaccgtgct cggccaagtc gcgctcgcgc gcggtgacgt cgcggaagca cgtcggcaac
     8281 tgcttgcctc tggtgcaaca cctggatcgc cgcag
//
LOCUS       DASUFX010000733        10642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_951842,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000733 DASUFX010000000
VERSION     DASUFX010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951842"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFV99_29585"
     CDS             <1..362
                     /locus_tag="VFV99_29585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063580.1"
                     /translation="FAGSATVTLAGVIMYVHGATKVSDAEDELCDRGGYPFNPGCQPA
                     DSSLTQDEVDRLNAKGSRGETIAMVGVGTMGVGLVMSVIAGYKGFIAKPKQQERAVTI
                     APTVSKDGAGATLQVRW"
     gene            447..578
                     /locus_tag="VFV99_29590"
     CDS             447..578
                     /locus_tag="VFV99_29590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063581.1"
                     /translation="MELWRRDADGWQRNDVTSGLVQLEAIDCTLAIDDVYYDPLATD"
     gene            603..1706
                     /gene="tgt"
                     /locus_tag="VFV99_29595"
     CDS             603..1706
                     /gene="tgt"
                     /locus_tag="VFV99_29595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5063582.1"
                     /translation="MLFDLPTPGFSFELLATLGHARAGILHTPRGDIPTPVFMPVGTA
                     GTVKAMTADELRAEPLDARIILGNTYHLYLRPGLEVIGAIGGLHKFAAWDRPILTDSG
                     GFQVFSLAAINEIDDDGVTFRSHIDGSKHRLTPEVSMEIQRVLGSDIAMVFDQCPPGD
                     AEEAVHEVALARTTAWAKRCREFKRAHGHAQALFGIVQGGIDVPRRMRHMAEITALDF
                     DGHALGGLAVGENLADTYRILDEVAHLLPAERPRYLMGVGTPADLEKGIGAGVDMFDC
                     VMPTRNARNGYLFTAGQRVNIPNNANRLDQGPIDPECPCSTCRTHSRAYLRHLYLAKE
                     ILYARLATLHNLTFYARHVRRIRDRILAEGTRC"
     gene            1812..2099
                     /gene="yajC"
                     /locus_tag="VFV99_29600"
     CDS             1812..2099
                     /gene="yajC"
                     /locus_tag="VFV99_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5063583.1"
                     /translation="MQPIMMMLVMFGIFYFILIRPQVKRQREHQSMLQKLGKGDEVIT
                     RGGVIGKITGISDDGVVVLELQEKVRVRIPRAYIEGRWDGKVPESAAKNAA"
     gene            2127..4136
                     /gene="secD"
                     /locus_tag="VFV99_29605"
     CDS             2127..4136
                     /gene="secD"
                     /locus_tag="VFV99_29605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5063584.1"
                     /translation="MDRSLKWRTAALVIVSLLCVCVVLPSFIPRENFPRWFNKIFTSK
                     MSMGLDLQGGLHLVYSIDLDKAVEDRASELKRDVESRFADDNIKGIVKTPSTPVGAVT
                     VVLEDGTKKAEVKATIEKDYGDTITTIDCPKGESANAICFRVSSKFADTIKKAALTNA
                     VTTIRDRINEKGVAEPNVVEKGDEIIVELPGLDKEMIDETKGIIARTAKLEMKVVDDC
                     TVYTPSGCTAANSTHEGSPYMKKLYAHVGSDRKGVATDPEAVKLDIQADIDQWRPDEG
                     GASHTDYYLLAPDRTESMPIAWAKKHGCPIPTGVTTGNIDCNVTGRQIIERYLYGDKD
                     LGYPGLIAADASFKAPDDRQLGFELIDPLPNAKDKRVKWRTYYLERAVRLTGSAISNA
                     MGSYDPNTNRPIVLLDFNRYGGRIFGDVTAQIVGMKFATILDDKVKSAPIINGAIRGG
                     RASITMGGSDPTVQEQERDDLVAVLKTGSLPAPLREESASEVGPSLGADAIAKTRLSF
                     ALGIALVILIMVGVYKWSGMIAVFAVVFHIVLTLAVMALFGATLTLPGIAAIVLSIGM
                     EVDGNILIYERIREELLLGKSVRGAIDLGFSRAFSAILDGQLTTAAAGWVLLNYGSGP
                     IKGFAVMLLVGVFTTVSTNIWVTRIFFDWAVHRHQKKGTMYTLSI"
     gene            4144..5436
                     /gene="secF"
                     /locus_tag="VFV99_29610"
     CDS             4144..5436
                     /gene="secF"
                     /locus_tag="VFV99_29610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5063585.1"
                     /translation="MAEDKPHPHKFRYLLPPGNNFHFVRKFRYWLIASVILMAASIAA
                     LFINHSVRGQFMNWTIDFKGGTEIIFVFKDTETGKFIEPDSAKVRAALQKAGDHAVEI
                     SGISYKEETANGTEDVHGLIVRTPRFSALKQEQRDAATKDFDETFKDREIGKATWSGD
                     RLFVRSKKPIKHEEAAALFQKLGLELKPWSDKEQEQYTHADEGTGEFNAVYAIWGLDR
                     QYGKVLESALPHTQATAVNSYGVGAKAGAKLRDDAAKSIIYAIFLIMLYLAFRFDIRY
                     APGAAFATLHDAVMVIGVFAVTWTEVSLTSVAGLLTVIGYSVNDTVIVFDRIRENQAK
                     LKDKKIDRIIDISLNEMLVRTILTSATVFATTLIMNIFGTGLVKNFAFAMNIGVIVGT
                     YSSIFLATPIFMWISRKYYTGAPKRRRGLPANAAEPTP"
     gene            5681..7396
                     /gene="recJ"
                     /locus_tag="VFV99_29615"
     CDS             5681..7396
                     /gene="recJ"
                     /locus_tag="VFV99_29615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5063586.1"
                     /translation="MQLRVRCLDDRLVDRIAGALRVRPATARCLVARGVVEPSDAQGF
                     IDPRLAALRPPSGLAGLSLAVERIAHAVERGERIGVFGDYDVDGITTAALLTSFLRAA
                     GAQVECAVARRDAGYGFTQEAVADFVTRGCKVVVTGDCGTSDLGALAAASERGIEVIV
                     VDHHTVPSASVAHPSFSLVNPYRADSTFPFRGMASVGLGFYVAASVRTELRDRGWFRS
                     RPEPDVRELLDLVALGTIADLVPLAAENRILTALGLRRLQSRTRPGIAALLASAGVDV
                     DKEIDAHTIAWKLAPRLNAPGRLGAAEPSLSLLLSDADAAPERAQALEAANKQRRSIQ
                     DKVMDEALEMIGDADPGPALVIAGEGWPSGVVGIVAAKLVDRYQRPAFVIGIDRETGI
                     GRGSARTCAGINLYEALATAAPMLDRFGGHAAAAGFTIKREAVPGLVEALGGAVTRLA
                     EGSGPVVAGVDVDAEVRLGEVDERLAQELAGLGPFGQANPAPLLVTRGARVKEVRRVG
                     DGQHLKLTLEDDQNVVRSAIGFKLGEREVEHGARLDLAFVPTISTWQGRRSAELEVAD
                     LSVVS"
     gene            7456..7830
                     /locus_tag="VFV99_29620"
     CDS             7456..7830
                     /locus_tag="VFV99_29620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063587.1"
                     /translation="MSDSPKAQPADDEPVPKLPRGRGLKLSGPELFRIALTLVALIGV
                     IVLTRPCATAVSGFVTSFDKGSNEKAMPKPGNVDMPREEQYEHLKPGMTEAEIKAAME
                     RSKARNAGSAAGSGSASAAPNP"
     gene            7862..8200
                     /locus_tag="VFV99_29625"
     CDS             7862..8200
                     /locus_tag="VFV99_29625"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5063588.1"
                     /translation="MSDERRASPRHTAHLAGELESEQGKSAIAITRDVSAGGLAVFTR
                     LRLTVGTPVKLLVLWKDEQVILNGTVLREQPLEPGESTLWRTKVAIGIDPKDPQFAKI
                     VAALAEEPAK"
     gene            complement(8204..9043)
                     /locus_tag="VFV99_29630"
     CDS             complement(8204..9043)
                     /locus_tag="VFV99_29630"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5063589.1"
                     /translation="MSWQLRVGAVQMRSSDDLKANLATVADLSAKAADQGAQLVALPE
                     CFSFLGRGEGDKLKIAEALSAGGPVMTALRELAIKHKVYMLGGGTPERVPGDTKRTYN
                     TAVVINPGGHLIAHYRKIHLFDVDIPGGATLKESDATAPGDELVVVEIGGARVGMTIC
                     YDVRFPELYRALVKDKGAEVLMVPAAFTAHTGAAHWHLLLRARAIEDQAWVVAPAQWG
                     RHNEKRESYGHTLVVDPWGTLVDEIPTGDGVLMATLHGDTVSQRRAQMPVLSHAVLWK
                     PRG"
     gene            9222..9842
                     /gene="rimP"
                     /locus_tag="VFV99_29635"
     CDS             9222..9842
                     /gene="rimP"
                     /locus_tag="VFV99_29635"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5063590.1"
                     /translation="MSRNPLHQRLITIIEPVLQAAGFELVDLRFVLEQGGWVLRVQVD
                     VPLEQVTDLSEVPSDRVDLSDCEDISRELSAVLDVEDPIPQAYHLEVSSPGIDRPLRT
                     VAHFAHFAGSEAKIQLAVPLHLEGGERKNFKGVLKGVIDDKVEIVCDGQPFQLLIADI
                     EHAKLVPDWDAVMHGRSGAGGAGAANQKKPIKPGHRPSQKHQKQKH"
     gene            9851..>10642
                     /locus_tag="VFV99_29640"
     CDS             9851..>10642
                     /locus_tag="VFV99_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEX5063591.1"
                     /translation="MEAKLDMVLEQVGRDKNIDKSVLINALEQAILTAAKRTFGMNRE
                     MEAKFNTETGSVDLFLIVNVVEEDDAIEGREITVEQARSAGLEAELGDELLFQVFYRA
                     EDEDRAREQDEKFGDLIDLKNASKKFGRIAAQTAKQVIYQRVREAERDNVYNEFKDRK
                     GEIISGVVRRFERGALIVDVGKAEAVLPMREQVPRESYRVGDTIKAYCLDIDRNARGP
                     QVILSRTHKGLLEKLFEQEVPEIYEKIVRIESSAREPGARAKIAVS"
BASE COUNT         1905 a         3461 c         3499 g         1777 t
ORIGIN      
        1 tcttcgctgg gtccgcgacg gtgacgctcg ctggcgtgat catgtacgtg cacggcgcga
       61 ccaaagtctc tgacgccgag gacgagctgt gcgaccgcgg cggttatccg ttcaatcccg
      121 gctgtcagcc cgcagactcg agcctcacgc aggacgaggt cgaccggctc aacgcaaagg
      181 gttcgcgcgg cgagaccatc gcgatggtcg gcgtcggcac catgggcgtc ggcctcgtga
      241 tgagcgttat cgcaggctac aagggcttca tcgcgaagcc gaagcaacaa gagcgcgcgg
      301 tcacgatcgc gccgaccgtc tcgaaggacg gcgccggcgc cacgctgcaa gtgcgctggt
      361 agtccgggtc tagtccgacc gccggccgcg tgtgtccgac gccctcgctt cgcgagcttg
      421 tcttggtcgc gcacgatcgg cggtcggtgg agctgtggcg gcgcgacgcc gacggctggc
      481 aacgcaacga cgtgacgagc gggcttgtcc agctcgaggc catcgactgc acgcttgcga
      541 tcgacgacgt ctactacgat ccgctcgcga ccgactaacg ctggggtata acgcgcagcc
      601 tcgtgctgtt cgatctcccg acgcccggct tctcgttcga gctcctcgcg acgctcggcc
      661 atgcgcgcgc gggcatcttg cacacgccgc gcggcgacat cccgacgccg gtgttcatgc
      721 ccgtcggcac cgccggtacc gtcaaggcga tgaccgctga cgagctgcgc gccgagccgc
      781 tcgacgcgcg gatcatcctc ggcaacacgt accacctgta cctgcgcccg ggcctcgagg
      841 tgatcggcgc gatcggcggc ctccacaagt tcgccgcgtg ggatcggccg atcctcaccg
      901 actccggcgg cttccaggtg ttctcgctcg cggcgatcaa cgagatcgac gacgacggcg
      961 tcacgttccg ctcgcacatc gatggctcca agcaccgact cacgcccgag gtctcgatgg
     1021 agatccagcg cgtgctcggc agcgacatcg cgatggtgtt cgatcaatgt ccgccgggcg
     1081 acgcggaaga ggccgtgcac gaggtcgcgc ttgcgcgcac gacggcgtgg gcgaagcggt
     1141 gccgcgaatt caagcgcgcg cacgggcatg cgcaggcgct gttcggcatc gtgcaaggcg
     1201 gcatcgatgt accgcgccgc atgcggcaca tggccgagat cacggcgctc gacttcgacg
     1261 gtcacgcgct cggtgggctc gccgtcggcg agaacctcgc cgacacgtac cggatcctgg
     1321 acgaggtcgc gcacctcttg cctgcggagc ggccgcgcta cctgatgggc gtcggcactc
     1381 ccgcggacct cgagaagggc atcggcgcgg gcgtcgacat gttcgattgc gtcatgccga
     1441 cccgcaacgc gaggaacggc tacctgttca cggcgggcca gcgcgtgaac attccgaaca
     1501 acgcgaaccg cctcgaccag ggaccgatcg acccggagtg cccgtgctcg acctgtcgca
     1561 cccactcacg cgcgtacctg cgacatcttt acctagccaa agagattctc tatgcgcggc
     1621 tcgcaactct gcacaacctc acgttctacg cgcggcatgt aaggcggatt cgcgatcgga
     1681 tcctcgctga aggcacgcgc tgctaagaat cagccacgcc tgcccgaccg aggagttgaa
     1741 gcaggcttga cccggcacca tacggatggt accaaccccc ggattttccg aggttagcgc
     1801 ccatgcaagc tatgcagccg atcatgatga tgttggtgat gtttggaatc ttctacttca
     1861 tcctgatccg cccccaggtg aagcgtcaac gcgagcatca atcgatgctg cagaagctcg
     1921 gcaagggcga cgaagtgatc acgcgtggtg gcgtgatcgg caagatcacg ggcatctccg
     1981 acgacggcgt cgtggtgctc gagctgcagg agaaggtccg tgttcgtatc ccgcgcgcct
     2041 atatcgaagg ccgctgggac ggcaaggtcc ccgagtctgc cgcgaagaac gccgcctgat
     2101 tcttttcgtt ctcgcaaagg tgcttcatgg atcgctctct caagtggcgc acagccgcgt
     2161 tggtgatcgt cagcctgctg tgtgtctgcg tagtcctacc tagtttcatt ccgcgcgaga
     2221 acttcccgcg ctggttcaac aagatcttca cctcgaagat gagcatgggg ctcgacctgc
     2281 agggcggcct ccacctcgtc tactcgatcg acctcgacaa ggccgtcgag gatcgcgcga
     2341 gcgagctcaa gcgggacgtc gagtcgcgct tcgcggacga caacatcaag ggcatcgtca
     2401 agacgccgtc gacgccggtc ggtgcggtca cggtcgtgct cgaggacggc accaagaagg
     2461 ccgaggtcaa ggcgacgatc gagaaggact acggcgacac gatcacgacg atcgattgcc
     2521 cgaagggcga gagcgccaat gcgatctgct tccgcgtgtc gtcgaagttc gccgacacga
     2581 tcaagaaggc cgcgctgacg aacgccgtca cgacgattcg cgatcgcatc aacgaaaagg
     2641 gcgtcgccga gccgaacgtc gtcgaaaagg gtgacgagat catcgtcgag ctgcccggtc
     2701 tcgacaaaga gatgatcgac gagaccaagg gcatcatcgc ccgtaccgcg aagctcgaga
     2761 tgaaggtcgt cgacgactgc acggtctaca cgccatcggg ctgcacggcc gcgaactcga
     2821 cgcacgaggg ctcgccgtac atgaagaagc tgtacgcgca cgtcggcagc gatcgcaaag
     2881 gcgtcgcgac cgaccccgag gcggtcaagc tcgacatcca ggcggacatc gaccagtggc
     2941 gtccggatga gggcggcgcc tcgcacaccg actactacct gctcgcgcct gatcgtaccg
     3001 agagcatgcc gatcgcgtgg gcgaagaagc acggctgccc gattcctacg ggagtcacca
     3061 ccggcaacat cgactgcaac gtcaccggcc gccagatcat cgagcgctac ctgtacggcg
     3121 acaaagatct cggctatccg ggcctcattg cggcggacgc gagcttcaag gcgcccgacg
     3181 atcgccagct cggcttcgag ctgatcgatc cgctgccgaa cgcgaaggac aagcgcgtca
     3241 agtggcgcac ctactacctc gagcgcgccg tccgtctcac cggctcggcg atctcgaacg
     3301 cgatgggtag ctacgacccg aacacgaacc gtccgatcgt gttgctcgac ttcaaccgct
     3361 acggcggccg catcttcggc gatgtcacgg cgcagatcgt cggcatgaag ttcgcgacga
     3421 tcctcgacga caaggtcaag tccgcgccga tcatcaacgg cgcgattcgc ggtggccgcg
     3481 cctcgatcac gatgggcggc agcgacccga ccgtgcagga gcaggagcgc gatgacttgg
     3541 tggcggtgct caagaccggc tcgctgccgg cgccgctacg cgaggaatct gcgagcgagg
     3601 tcggtccgtc gctcggcgcc gatgcgatcg cgaagacgcg gctgtcgttc gcgctcggca
     3661 tcgcgctcgt catcctcatc atggtcggcg tctacaagtg gtcgggcatg atcgcggtgt
     3721 tcgcggtcgt cttccacatc gtgctgacgc tcgcggtcat ggcgctgttc ggtgcaacgc
     3781 tcacgttgcc tggcatcgct gcgatcgtgc tgtcgatcgg catggaggtc gacggcaaca
     3841 tcctcatcta cgagcgcata cgcgaagagc tgttacttgg aaaatcggtg cgcggcgcga
     3901 tcgacctcgg gttctcgcgc gcgttctcgg caatcctcga cggccagctc acgactgccg
     3961 ccgcaggctg ggtgcttctc aattacggct cgggtccgat caagggcttt gccgtgatgc
     4021 tcctcgtcgg cgtcttcacc accgtctcca ccaacatctg ggtcacgcgc atcttcttcg
     4081 attgggccgt tcaccggcat cagaagaagg gcacgatgta caccctgtcg atctgaggct
     4141 gccatggctg aagacaaacc ccatccgcac aagttccgct acctgcttcc gccgggcaat
     4201 aacttccact tcgtccggaa gttccgttac tggctgatcg cgtcggtcat cctgatggcc
     4261 gcgagcatcg cggcgctgtt catcaaccat tccgttcgcg gccagttcat gaactggacg
     4321 atcgacttca agggcggcac cgagatcatc ttcgtgttca aggacacgga gaccggcaag
     4381 ttcatcgagc ccgactccgc caaggtccgc gcggcgctcc agaaggccgg cgaccacgcg
     4441 gtcgagatct cgggcatctc gtacaaggaa gagaccgcga acggcaccga ggacgtgcac
     4501 ggcctcatcg tccgcacgcc gcgcttctcc gcgctcaagc aggagcaacg cgacgccgcg
     4561 acgaaggact tcgacgagac gttcaaggac cgcgagatcg gcaaggcgac gtggtcaggc
     4621 gaccgcctgt tcgtgcgctc gaagaagccg atcaagcacg aggaagcagc ggcgctgttc
     4681 cagaagcttg gcctcgagct caagccctgg tctgacaagg agcaggagca gtacacgcac
     4741 gccgacgagg gcaccggcga gttcaacgcg gtctatgcga tctggggcct cgatcgtcag
     4801 tacggcaagg tgctcgagag cgcgctgccg cacacgcagg cgaccgcggt gaatagctac
     4861 ggcgtcggcg cgaaagccgg cgcgaagcta cgcgacgacg ctgccaagtc gatcatctac
     4921 gccatcttcc tgatcatgct gtacctcgcg ttccggttcg acatccggta cgcgccaggc
     4981 gcagcgttcg cgacgctgca cgacgcggtc atggtcatcg gcgtgttcgc cgtgacctgg
     5041 accgaagtct cgctgacgtc ggtcgccggt ctcctcacgg tcatcggtta ctccgtaaac
     5101 gacaccgtga tcgtcttcga ccgaatacgc gagaatcagg cgaagctcaa agacaagaag
     5161 atcgatcgca tcatcgacat ctcgctcaac gagatgctcg ttcgcacgat cctcacgtcg
     5221 gcgacggtgt tcgcgaccac gctgatcatg aacatcttcg gcacgggcct cgtgaagaac
     5281 ttcgcgttcg cgatgaacat cggcgtgatc gtcggtacgt attcgtcgat cttcctcgcg
     5341 acgccgatct tcatgtggat ctcgcggaag tactacaccg gtgctcccaa gcgccgccgt
     5401 ggccttcccg cgaacgccgc cgaaccgacg ccgtaaccgc ggcctgagtg ggctatggcg
     5461 tcaggcgcgg atgcgttagc cgccacagca gagcccgtaa gcgatcgatc agctggcacc
     5521 tcgcgcgtcc gaggcgcggc cacgcgcgtc tcgcgatgcg gacaaatggt ccgcactgcg
     5581 gacgtagtgc gttcacccga tcccgggatc atctaattca cagggatcac tcggggatct
     5641 gctgggtttc ggtgtcagac ccgacgacta gcgtccgcgt gtgcagttgc gcgttcgctg
     5701 tctcgacgac cgactcgtcg atcgcattgc cggtgcgctg cgtgttcgcc cggcaactgc
     5761 tcgctgtctc gtcgcgcgcg gcgtcgtcga gccaagcgat gcgcaaggct tcatcgatcc
     5821 gcgcctcgcg gcactgcgcc cgccgtcggg tcttgcgggg ctatcgctcg ccgtcgagcg
     5881 catcgcgcat gcggtcgagc gtggcgagcg tatcggcgtg ttcggcgact acgacgtcga
     5941 cgggatcacg accgcagcgc tactgacgtc gtttctgcgc gcggcgggtg cgcaggtcga
     6001 atgcgccgtc gcgcgacgcg atgctggcta cggcttcacg caggaagcag tcgcggattt
     6061 cgtgacgcgt ggctgcaaag tggtcgtcac gggtgactgc ggcacgagcg acctcggtgc
     6121 actcgcggcc gcgagcgagc gcggtatcga agtgatcgtg gtcgatcacc atacggtccc
     6181 atcggcgtcg gtggcgcatc cttcgttctc gctcgtgaat ccgtaccgcg ccgattcgac
     6241 gttcccgttt cgcggcatgg cgtcggtggg gctcgggttt tacgtcgcag catcggtacg
     6301 cacggagctg cgcgatcgcg gctggttccg gtcgcggccg gagcccgacg tgcgcgagct
     6361 gctcgacctg gtcgcgctcg gcacgatcgc cgacctcgtg ccgctcgcgg cggagaatcg
     6421 gatcctgacc gcgctcggcc tgcggcgcct gcagagcagg acgcgccccg gcattgcagc
     6481 gctgctcgcg agtgcgggcg tcgacgtgga taaagagatc gacgcgcaca cgatcgcgtg
     6541 gaagctggcg ccgcggctca atgcacccgg acggctcggt gcggcggagc cgtcgctgtc
     6601 tttgctgctg tcggatgcgg acgccgcgcc ggagcgcgcg caggcactcg aggctgcgaa
     6661 caaacagcgc cgctcgatcc aggacaaggt gatggacgag gcgctcgaga tgatcggcga
     6721 cgctgatccg gggcctgcgc tcgtgatcgc gggcgaaggc tggccgtcgg gcgtcgtcgg
     6781 catcgtcgcc gcgaagctcg tcgatcgcta tcagcggccc gcgttcgtga tcggcatcga
     6841 tcgcgagacc ggcatcggtc gcggctcggc gcgcacgtgc gcgggcatca atctgtacga
     6901 agcgctcgcg accgcggcgc cgatgctcga tcggtttggc ggtcacgcgg cggcagcggg
     6961 cttcacgatc aagcgcgaag ctgtgcccgg gctcgtcgaa gcactcggcg gcgcagtcac
     7021 gcggctcgcg gaaggctcgg ggccggtcgt cgctggcgtc gacgtcgatg ccgaagtgcg
     7081 gctcggcgaa gtcgacgagc ggctcgcgca ggagctcgcg ggcctcgggc cgttcggcca
     7141 ggcgaatccg gcgccgctcc tcgtcacgcg cggcgcgcgg gtaaaagaag tgcggcgcgt
     7201 cggcgacggg cagcacctca agctcaccct cgaagatgac cagaacgtcg tgcgcagcgc
     7261 gatcggcttc aagctcggcg agcgcgaggt cgagcacggg gcacgcttgg atctcgcatt
     7321 cgtgccgacg atctcgacgt ggcagggtag gcggagcgct gagctcgagg tcgccgacct
     7381 gtcggtcgtg tcgtaggcgc gcccgcgcaa cgctcgcatc tggcacgtgt ctgttcttcg
     7441 aggtatccac tacccgtgag cgattcgccg aaagcgcagc cggccgatga cgagccggtg
     7501 ccgaagctgc cgcgtggccg tgggctcaag ctgtcgggcc ccgagctgtt ccggatcgcg
     7561 ctcacactcg tcgcgctgat cggcgtgatc gtgctgacgc gcccgtgcgc gaccgcggtg
     7621 tcgggcttcg tgacgagctt cgacaagggc agcaacgaaa aggcgatgcc caagcccggg
     7681 aatgtcgaca tgccgcgtga ggagcagtac gagcacctaa agcccggcat gacggaagcc
     7741 gagatcaaag ccgcgatgga gcgtagcaag gcgcgcaacg caggcagcgc tgccggtagc
     7801 gggagcgcgt ccgctgcgcc gaacccgtag cgacggttcg cgagcggtgt atgctggcac
     7861 cgtgagtgac gaacggcggg catctcctcg acacaccgcg catctcgcgg gggagctcga
     7921 gagcgaacaa ggcaagtcgg cgatcgcgat cacgcgcgat gtcagcgcgg gcggtctcgc
     7981 ggtgttcacg cggctgcggc tgaccgtcgg tacgcccgtg aagctgctcg tgctgtggaa
     8041 agacgagcag gtgatcttga acggcacggt gctgcgcgag cagcccctcg agccaggcga
     8101 gagcacgctg tggcggacga aggtcgcgat cggtatcgat ccgaaagatc cgcagttcgc
     8161 gaagatcgtc gctgcgctcg ccgaagaacc ggcgaagtag cgcttagccg cgtggcttcc
     8221 agagcacggc gtgcgagagc accggcatct gcgcgcgccg ctgcgagacg gtatcgccgt
     8281 gtagcgtcgc catcagcacg ccatcgccgg tcgggatctc gtccacgaga gtgccccacg
     8341 gatcgacgac cagcgtgtgg ccgtagctct cgcgcttctc gttgtggcgt ccccactgtg
     8401 cgggcgcgac gacccacgcc tgatcctcga tcgcgcgagc acgcaaaagc aggtgccagt
     8461 gcgccgcacc ggtgtgcgcg gtgaacgcgg ccggcaccat caggacctcg gcgcctttgt
     8521 ctttgacgag cgcgcgatag agctcgggga accggacgtc gtagcagatc gtcatgccga
     8581 ctcgagcgcc accgatctcg acgacgacca gctcgtcgcc cggtgcggtc gcgtccgact
     8641 ccttgagcgt cgcgccgccc gggatgtcga cgtcgaacag gtgaatcttg cggtagtgcg
     8701 cgatcaagtg accgccggga ttgatgacga ccgccgtgtt gtacgtgcgc ttcgtatcgc
     8761 ccggcacgcg ctcgggggtg ccgccgccga gcatgtagac cttgtgcttg atcgccagct
     8821 cgcgaagcgc ggtcatgacc ggcccgccgg cactgagcgc ctcggcgatc ttgagcttgt
     8881 cgccttcccc gcggccaagg aacgaaaagc actcgggaag tgcgacgagt tgcgcgccct
     8941 ggtcggcggc cttcgcactg aggtccgcga ccgtcgcgag gtttgccttg aggtcgtcgc
     9001 tcgacctcat ctgcacggcc ccgacccgta gctgccagct catgacccta gggtatgcgc
     9061 ttgccaaaac gccgcaagtt gctgcatcga caggctgccc ggatcggcaa gcctacgcaa
     9121 aggcacttgc tatgcggttt ggccgatggt acccatcccg cagcttcaat cgcttcgcgg
     9181 tagaggtggc cttccggcca cctttttttt cgccttaaac catgtcccgc aatccacttc
     9241 accagcgctt gatcacgatc atcgagcccg tcctccaggc ggcgggcttc gagctggtcg
     9301 acctgcgctt cgtgctggag cagggcggtt gggtgctccg cgtccaggtc gacgtgccgc
     9361 tcgagcaggt gacggatctt agcgaagttc cgagcgaccg cgtcgacctc tccgattgcg
     9421 aggacatctc gcgcgagctc tctgcggtac tcgacgtcga ggatccgatt ccgcaggcgt
     9481 atcacctcga agtcagctcg ccgggcatcg atcgtccgct gaggaccgtt gcgcacttcg
     9541 ctcacttcgc gggcagcgaa gcgaagatcc agctcgcggt cccgcttcat ctcgagggcg
     9601 gcgagcgcaa gaacttcaag ggcgtcttga agggcgtcat cgacgacaag gtcgagatcg
     9661 tctgcgacgg ccagccattc cagctgctga tcgccgacat cgagcacgcc aagctcgttc
     9721 ccgactggga cgccgtcatg cacggccgat caggagcggg cggggcaggg gccgccaacc
     9781 agaagaaacc gatcaagccg ggacaccggc ctagtcagaa acaccagaag cagaagcact
     9841 gaggaacgcc atggaagcca aactcgacat ggttctcgag caggtaggcc gagacaagaa
     9901 catcgataag agcgtgctca tcaacgcgct cgagcaagcc attctcacgg ccgccaagcg
     9961 cacctttggc atgaaccgcg agatggaagc gaagttcaac acggagacgg gctctgtcga
    10021 cctgttcctc atcgtcaatg tcgtcgagga agacgatgcg atcgaaggtc gcgagatcac
    10081 ggtcgagcaa gcgcgctcgg cgggtctcga ggcggagctc ggcgacgagc tcttgttcca
    10141 ggtgttctac cgcgccgagg acgaagaccg cgcgcgcgag caggacgaga agttcggcga
    10201 cctgatcgac ctcaagaacg cgagcaagaa gttcggccgc atcgcggcgc aaaccgcgaa
    10261 gcaggtcatc taccagcgcg tgcgcgaggc cgagcgcgac aacgtctaca acgagttcaa
    10321 ggatcgcaag ggcgagatca tcagcggcgt cgtgcgccgc ttcgagcgcg gcgcgctgat
    10381 cgtcgacgtc ggcaaggccg aggccgtgct gccgatgcgc gagcaagtgc cgcgcgagag
    10441 ctatcgcgtc ggcgacacga tcaaggcgta ctgcctcgac atcgatcgca acgcgcgcgg
    10501 cccgcaggtc atcctgtccc gcacgcacaa gggcttgctc gagaagctgt tcgagcaaga
    10561 ggtgccggaa atctacgaga agatcgtgcg catcgagtcg agcgcgcgcg agcccggtgc
    10621 acgcgcgaag atcgctgtgt cg
//
LOCUS       DASUFX010000734        11985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1922857, whole genome shotgun sequence.
ACCESSION   DASUFX010000734 DASUFX010000000
VERSION     DASUFX010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922857"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFV99_29645"
     CDS             <1..628
                     /locus_tag="VFV99_29645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063592.1"
                     /translation="IANCDRVLRDITKTGVRGFRAPGYDISPAMLDTLARLGYRYDSS
                     VFPAPGYYAAKAAVMTALEVLGRPSGAVLTNPRALAAPAEPYRPSMSAPWRRGQAPVV
                     ELPVAVTPYARVPAIGTTLLVAPPWVRAKLIDWMARRSFFNFELHGIDFADADKDGIP
                     GELVNHQPDLRISIDDKLARLDAVLDEIARRWEFATLADVSADVQRTE"
     gene            689..1150
                     /locus_tag="VFV99_29650"
     CDS             689..1150
                     /locus_tag="VFV99_29650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5063593.1"
                     /translation="MSSAKSDGGKEKGELIPYTLDNPFAFMRELFEQFDFAMPRVDIE
                     HQDDQLDIQIDLPGISPEDILITMDEYLLTLEGVRYERRRREEGGVMRGERTFGKFQR
                     VMPLPQRVDPYTAQATFENGVLAIKVCTIGSRPEARKVPISVVGREPEPTD"
     gene            1156..1704
                     /locus_tag="VFV99_29655"
     CDS             1156..1704
                     /locus_tag="VFV99_29655"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX5063594.1"
                     /translation="MTDQPTWRWWTIALRGLATVLFGALAIAIGLTGWLVLLFGAYAL
                     IDGVLAISIANAKVDKPRGVIMARGLVSIGAALVTLLWPGMSRFALAIVIALWAIAAG
                     VLEMATASRMRKELRHEWLTGFEGMLSVSFGWALLVATLAGVRALAVWVETYAFVLGA
                     MLLLTAFQMRSYVRQRPAIAAA"
     gene            complement(1688..2335)
                     /locus_tag="VFV99_29660"
     CDS             complement(1688..2335)
                     /locus_tag="VFV99_29660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEX5063595.1"
                     /translation="MPWIKHRLRDADVWAKVDAGGQLVTDRDGRVEIVYKPVDGAKTY
                     RAGARNLSPVAGEKPVELEAGERAPDKQQQASQASQPAADAIQVWTDGACSGNPGPAG
                     LGVVILDGKHRTELSEYLGEGTNNIAELTAILRGLETLKDKSRPVVVYSDSSYSIDIL
                     TKAWKAKKNVELIDELRAVCRTFADLRFVKVAGHAGVPLNERCDQLAREAITRRR"
     gene            2398..3432
                     /locus_tag="VFV99_29665"
     CDS             2398..3432
                     /locus_tag="VFV99_29665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063596.1"
                     /translation="MVRTRSVLIAVVALGLGAGACKKKEEAKGGAPADKTTEKGGEIT
                     KAAPSGGVIAGADDLSLLPADSEIVMGLNFAQLQQSALWKQFAPKLMEKAASGLAEFK
                     AACGFDPIEQIKSVSLGMKGVDGGKTPEGAVVIHGLDKGKSMACIDKAKAEAAKKGSD
                     ITVDGEVFTVKDKNGQLTAWTFVGGDTLLGVLGPSASKDTVLAAAKGGSALKTSQTFV
                     DMYSKINTKESLWLLINGNAPFMAKAASAGVKPKAVFGSVNVTDGLTVDLRIRLATPD
                     EATNLVKMMQGQTNNPQVKQMFDKFDVTADGADAKIAIAMSNQKLQQLIGMIGGMMGG
                     MMGGGGMGGP"
     gene            3452..4474
                     /locus_tag="VFV99_29670"
     CDS             3452..4474
                     /locus_tag="VFV99_29670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063597.1"
                     /translation="MKLATLVAAAALVIGGCGNKKSKALPEVSGLAAVPASATAVVVA
                     DVARVIDAPLVERAVDQLLLRDPVLRDRWQGLYNNCKLDARKLKHVILAIGPHEPQAG
                     TGPVIMVVTGQIAENELASCVRTIVGQGGGQLTAKEISGRTLYQAKDGNRTMFFAFGK
                     PDTVILGSNEAYVTEALGTGKKVTDNAALVGWMKLADQSAPVWAAGQVDERVRPGLVK
                     LTDGQVKEGPVAMVMSANPAKGLELSIGAVMASEADAKTLESFAKTQLGLVAMAAQAK
                     KLGRVVDKVTISAEKQVVRFKAPLTDEDVNLIISALDGGGITAQDSPPATPGSASTAG
                     AAGPQR"
     gene            4481..4924
                     /locus_tag="VFV99_29675"
     CDS             4481..4924
                     /locus_tag="VFV99_29675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063598.1"
                     /translation="MASKKTTSKKKIDTKKPAKKAAAPKKAAKEKTEAKPVGPRHPAG
                     RVAHHHNGKEALAKALAPMLAREDEDNSVIADRLKTASNAQLLRLQRVADAVKQKWGS
                     REALITALSTAEKKGKDKDYLAKLGTFSLPKLFDLATSAERRARA"
     gene            4946..6640
                     /locus_tag="VFV99_29680"
     CDS             4946..6640
                     /locus_tag="VFV99_29680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5063599.1"
                     /translation="MPARVLIVVLLLASRARAESVAPPPVTSGVQLVRADAQWRYQIF
                     TAPQLAPQIGALAISGLDVALGRAQSPIAVLGDEADAPVAWPYDVDAATVANGPLAPT
                     ASDARIAALFAVTPFTLTHEHQGLRVLEIRLRYHDGCAVWLNGSEVARRSLDGGTVAL
                     AARPHGPEWETFYVSVAPGLLRLGENVLAIEVHPSGRSNAPDLAADVVGRRELGLVRG
                     PIITDIGATSATISIETDANLDAALEWGTGQTLDHRLTSAPGRMHRFKLDGLPPRTAI
                     SYRVHAGASQSPQFAFHTTPPAGELIRIGIYGDVRGGHDTHRKLVEQMLGEGLDLVAV
                     TGDMVMRGTDNGDWQKFFAVTRELLATVRYLPAIGNHDLGWRRADPDVFAFPTAPAGR
                     PDATYWYSLDVADIHLVFLDSNAYERTEQEQWLDADLAAARARGARAIIALTHDGPYS
                     RGVHRGSQIARERYVPILAKYHVDLLVGGHDHLYQRGEAGGIHYIVSGGGGAGLYQPT
                     CGVGRKPKCAVDGMQKVASEHHYLVLAIDKETIEMCPRRPDGKLLEKCTRYGLWRP"
     gene            6668..7600
                     /locus_tag="VFV99_29685"
     CDS             6668..7600
                     /locus_tag="VFV99_29685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063600.1"
                     /translation="MRFVLVALAVVGCHHNPGTPGPSTPANKAAAPDYRTTADDELGF
                     LPVGTDIVIGMNVTQFRGTAMWNSLQPQIDGFRRELEQIVGDCSHPNPIDTIERGTVA
                     INLAPDGNPHGVFVVRGIDTTRVLDCMIQQMKKEGGTGMLDRGVAIMSMPGSRTQLAA
                     AVVGPSTLVMQTETTVTYDSMTKILTSGSPLRSSPVFMEFYKRREAGASFWGMANGNA
                     WFFEELAQMGMRPKSLDGTLIVTDRLAFSIRMTMASPADAAKVSAELDKIKGPAGGFV
                     QQFDTRIDDATLRIHVVMTEQQVRGLAGMLGGMP"
     gene            complement(7601..8743)
                     /locus_tag="VFV99_29690"
     CDS             complement(7601..8743)
                     /locus_tag="VFV99_29690"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5063601.1"
                     /translation="MPSKWTVEELAPGRTVEAIFWPNPAEGSTFKLRATHLDGRRAPK
                     VVLSNDDRITPGVPCLVRVLAVHKPERDDRGRIEVEFVARAPFRIEGMYLDPIVSKKL
                     QVLLQSGLNILLDGPQGCGKTVLARAIADSLGMVFVFFNCGAVVEASDFFVSIQVRAS
                     ASGAPVTDFIKTDVLVALEEAAEHPEHKYLVFLDELNRCQESARNALMPALDATRRVY
                     HPIENTFIPIPDNVQFIAAVNRGREFTGTFGIDAAQLDRFAPLQMDYPPPKAEVEILA
                     KRHPELSAANLEIIVGVADAIRSAADLPGSLSVRATDEACVYLEHPLFADDGKRALPE
                     ILKTSFCGRFPGRWDDVTTDAGAVWSIVRKTLATLKVPLPEDGGKE"
     gene            complement(8756..>11985)
                     /locus_tag="VFV99_29695"
     CDS             complement(8756..>11985)
                     /locus_tag="VFV99_29695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5063602.1"
                     /translation="GHISGVCAALSERSPGSSVAACNALIGCADPLQDVARTLDRFAE
                     PTPQFDADLDNAAVRWVRVKELPVLAHARLWRWEAHSFALLDWIDAVGGPYHALQVAE
                     ELPGRLARHTLWKAISEAMMLIRYRTPDRFKHLASITLGQYCAEAIDRDIGRHAARLL
                     VALVESKTIALVEIRDRMLDRIADADAEAREYLARLVRLDGIPDPPRTAQLPSSSLLE
                     QIRASTDLDALTRWCADPRPAIVQEAALALVVLGADGQARLAGLLAELELLAAPIPLL
                     ATIPLWDHAPALAAVRALSTKPDLPPAWQFHISLALAQRGEGDALLRAVAAVRAPPKG
                     WYFRREDWDALVKVGHVVAISIGLCDSPHHHAYQRAINTLLSLTRPTWTVQDGLTRFL
                     EVGDDRPLHLRVSVARFLAQHFGDPVGLPLLAEYIADERADDWLYTLELVPREHMAAV
                     AEMLVSAALVGGPLVCSEKRMWTIVQRMRDEGFLDHATQTDIDLRIFEQAALTATRRA
                     ASKYAVTASAGDARLRRVADVFAWGIRRGVELTGRLFRIHMTAKEKDLGHTKLDGNHI
                     FVSPLPMLRGETYGQDIVEGLVLHELGHHVYHRSEEAQALWKQAHQEGIGHFLNLIAD
                     EHLERNLRALDPEYGDRLKRLGAYAFQHAPQEMKVAVLFDALGASTARAFTNGELEVA
                     FDEESVRVRRGAILGELDRGGHPVARFSRALRMGLGNRAGDPLIEKALALCGKDLRGL
                     DMKGLYNLTNQLVELFGGKHQIAKVFGGPEGLIFGDREDDVFGAGVDDDILQREVERI
                     LDPRRSKRGPPQPGSRLQLNVSPDIKFNPINRIVRVRGDADVHRKLATDVSRHAHRLR
                     QFLDELGLRWVPQHARTQGRALDKTRLTPLVTRNDPRILIARNPQRRTDLFLGTLVDC
                     SSSMTAGQNIERAKRFAVLVSEAVRTLPGVEARFFGFTDSVIYDAGSANDCGVVGLHA
                     SGGNNDAAALYHAANVALNAKQRAKVLVMISDGLPTECSVTALRGLVTTLTRRRNIVC
                     AQVAVRALDEVCFPHYIVLDDNQPDIAVAKFGRMIGELARRSLSS"
BASE COUNT         2041 a         3829 c         4177 g         1938 t
ORIGIN      
        1 gatcgcgaac tgcgatcgcg tgttgcgcga catcacgaag actggtgtgc gtggctttcg
       61 cgcgcccggc tacgacatct cgccagcgat gctcgatacg ctcgcgcggc tcggctaccg
      121 ctacgactcg tcggtgtttc cggctcccgg ctactacgcg gcaaaggcgg cagtgatgac
      181 ggcgctcgag gtgctcggcc ggccgagcgg cgcggtgctg acgaatccgc gtgcgctcgc
      241 cgcgcccgcg gagccgtatc ggccgtcgat gtcggcgccg tggcggcgag gccaagcgcc
      301 ggtcgtcgag ctgccggtcg cggtgacacc atacgcacgc gtgccggcga tcggcaccac
      361 tctgctcgtc gcgccgccgt gggtgcgagc gaagctgatc gactggatgg cgcgccgctc
      421 gttcttcaac ttcgagcttc acggcatcga tttcgcggat gctgacaagg acggcatccc
      481 gggcgagctc gtgaaccatc agcctgatct ccgcatttcg atcgacgaca agctcgcgcg
      541 actcgatgcg gtgctcgacg agatcgcgcg gcgctgggaa tttgcgaccc tggcggacgt
      601 gtcggccgac gtccagcgca ccgaataacc cgcgcacgcg gaaactggtt cgcgcgatgc
      661 acgctccgcg ctgcagagga cccagatcat gagctcagca aaatccgatg gcgggaagga
      721 aaaaggcgag ctgattccct acacgctgga caatccgttc gcattcatgc gcgaattgtt
      781 cgaacagttc gacttcgcga tgccgcgggt cgacatcgag catcaggacg atcagctcga
      841 catccagatc gatctaccgg ggatttctcc cgaggacatc ttgatcacga tggacgagta
      901 cctgctcaca ctcgaaggcg tgcgctacga gcggcggcgg cgcgaggagg gtggcgtgat
      961 gcgtggcgag cgtacgttcg gcaagttcca gcgcgtgatg ccattgccac agcgcgtgga
     1021 tccgtacacg gcgcaggcga cgttcgaaaa cggcgtgctc gcgatcaagg tgtgcacgat
     1081 cggcagtcgt cccgaggctc gcaaggtccc gatcagcgtg gtcggccgcg aaccggaacc
     1141 gacggactag gtgtcgtgac cgaccaaccg acatggcgct ggtggacgat cgcgttgcgc
     1201 gggctcgcga cggtgttgtt cggcgcgttg gcgatcgcca ttggcctcac aggctggctc
     1261 gtgctcttgt ttggcgcgta cgcgctcatc gatggcgtgc tcgcgatttc gatcgcgaat
     1321 gccaaggtcg acaagccgcg cggcgtgatc atggcgcgcg gcctcgtgtc gatcggcgct
     1381 gcgctcgtca cgctgctgtg gcccggcatg tcacggttcg cactcgcgat cgtgatcgcg
     1441 ctgtgggcga tcgccgccgg cgtgctcgag atggcaaccg cgagccgcat gcgcaaggag
     1501 ctacggcacg agtggctgac cggcttcgag ggcatgctgt ctgtctcgtt cggatgggca
     1561 ctcctcgtcg cgacactcgc aggcgtgcgc gcgctcgccg tttgggtcga gacgtacgca
     1621 ttcgtgctcg gcgcgatgct gctcttgacc gcgttccaga tgcggtcgta cgtgcggcag
     1681 cggccggcta tcgccgccgc gtgatcgctt cgcgcgcgag ctgatcgcaa cgctcgttga
     1741 gcggcacacc agcgtgaccg gcgaccttga cgaagcgcag gtcggcgaac gtccggcaca
     1801 cggcgcgcag ctcgtcgatc agctcgacgt tcttcttcgc cttccacgcc ttcgtgagga
     1861 tgtcgatcga gtacgacgag tcggaataga cgacgaccgg ccgcgacttg tccttcaagg
     1921 tttcgagccc gcgcaggatc gcggtgagct cggcgatgtt gttcgtgcct tcgccgagat
     1981 actccgacag ctcggtgcga tgtttcccgt cgaggatgac gacgccaagc ccggcgggac
     2041 cgggattgcc gctgcacgcg ccgtcggtcc agacctggat cgcgtccgcg gcgggctgcg
     2101 acgcctgcga cgcttgctgc tgcttgtcgg gcgcacgctc acctgcctcg agctcgaccg
     2161 gtttttcacc ggctaccggc gacaaattgc gtgcgcctgc gcggtaagtc ttggcaccgt
     2221 cgacgggctt gtaaacgatc tccacacggc cgtcacgatc ggtgacaagt tggccgccag
     2281 cgtcgacctt cgcccacaca tcagcgtcgc gcagacggtg cttgatccat ggcatgggga
     2341 tgcgcccgtg ttataagccc agcgtccgat ctcggacgct atcaggagag agaaaacatg
     2401 gtccgcacgc gcagtgtcct catcgccgtc gtcgcactcg gcctcggcgc cggtgcttgc
     2461 aaaaagaaag aagaagccaa gggtggtgca cctgcggaca agaccaccga gaagggtggc
     2521 gagatcacga aggctgctcc gtcgggtggc gtgatcgcgg gcgcggatga tctctcgctg
     2581 cttcccgcgg actccgagat cgtcatgggc ctgaacttcg cgcagctcca gcagagcgcg
     2641 ctgtggaagc agttcgcgcc gaagctgatg gagaaggccg cgtcgggtct cgccgagttc
     2701 aaggcggcct gcggcttcga tccgatcgag cagatcaaga gcgtgtcgct cggcatgaag
     2761 ggcgtcgatg gcggcaagac ccccgagggc gcggtcgtca ttcacggcct cgacaagggc
     2821 aagtcgatgg cgtgcatcga caaggcgaag gccgaagccg cgaagaaggg ctcggacatc
     2881 acggtcgacg gcgaagtctt caccgtcaag gacaagaacg gtcagctcac cgcgtggacg
     2941 ttcgtcggtg gcgacacgct gctcggcgtg ctcggcccgt cggcgagcaa ggacacggtt
     3001 ctcgcggctg ccaagggcgg ctcggcgctg aagacgtcgc agacgttcgt ggacatgtac
     3061 tcgaagatca acacgaagga gtcgctgtgg ctcctcatca acggcaacgc gccgttcatg
     3121 gcgaaggccg catcggccgg cgtcaagccg aaggctgtgt tcgggtcggt caacgtgacg
     3181 gacggtctca ccgtcgatct acgtatccgc ttggcaaccc cggacgaggc gaccaacctc
     3241 gtcaagatga tgcagggcca gacgaacaac ccgcaggtca agcagatgtt cgataagttc
     3301 gacgtcacgg ctgatggcgc ggacgcgaag atcgcgatcg ccatgagcaa tcagaagctc
     3361 cagcagctga tcggcatgat cggcggcatg atgggcggca tgatgggtgg cggcggcatg
     3421 ggcggtccgt aaactagacc tgggtaggga catgaagcta gcgaccctcg tggcggctgc
     3481 ggccctcgtc attgggggct gcggcaacaa gaagtccaag gcactgcccg aggtctctgg
     3541 ccttgcggcg gtgccggcat cggcgacggc cgtggtcgtt gcggatgtcg cgcgcgtgat
     3601 cgatgcgccg cttgtcgagc gcgcagtcga tcagctgctg cttcgcgatc cggtgctccg
     3661 cgatcgctgg cagggcctgt acaacaactg caagctcgac gcgcggaagc tgaagcacgt
     3721 catcctcgcg atcggcccgc acgagccgca agcgggcacc ggtccggtga tcatggtcgt
     3781 cacgggccag atcgccgaga acgagctcgc gagctgcgtg cgaacgatcg tcgggcaggg
     3841 cggcggccag ctgaccgcga aagagatcag cggccgcacg ctctatcaag cgaaggacgg
     3901 caatcgcacg atgttcttcg cgttcggaaa gcccgacacc gtgatcctcg gctcgaacga
     3961 ggcgtacgtg accgaggcac tgggcaccgg taagaaggtc accgataacg ccgcgctggt
     4021 cggctggatg aagctcgcag atcagagcgc gccggtctgg gcggcgggtc aggtcgacga
     4081 acgagtgcgg ccgggcctcg tgaagctgac cgacggccag gtcaaagagg gaccggtcgc
     4141 gatggtgatg agcgcgaacc cggccaaagg actcgagctt tcgatcggcg cggtgatggc
     4201 gtcggaggcg gatgccaaga cactggaatc gtttgcgaag actcagctcg ggttggtcgc
     4261 gatggccgca caggccaaga agctcgggcg ggtcgtcgac aaggtcacga tttcggctga
     4321 gaaacaggta gttcggttca aggcgccgct gaccgacgag gacgtcaatc tgattatttc
     4381 ggcgcttgac ggcgggggta tcaccgcgca ggattccccg cccgcaaccc ccggcagtgc
     4441 aagtaccgcc ggagccgcgg gcccgcagag gtagatgatc atggcgagca agaagacgac
     4501 gtcgaagaag aagattgata cgaagaagcc cgcgaagaag gcggcggcac ccaagaaggc
     4561 ggcgaaggaa aagaccgagg cgaagccggt cggtccgcgt cacccggcgg gccgcgttgc
     4621 ccaccaccac aacggcaagg aagcgctcgc gaaggcgctc gcgccgatgc ttgctcgtga
     4681 agacgaagac aacagcgtga tcgcggaccg tctcaagacg gcgtcgaacg cgcagctcct
     4741 ccgcctgcag cgcgtcgccg acgcggtcaa gcagaagtgg ggcagccgcg aagcgctgat
     4801 caccgcgctc agcacggccg agaagaaggg caaggacaag gattacctcg ccaagctcgg
     4861 cacgttctcg ctgccgaagc tgttcgatct cgcgacctcg gcagagcgtc gcgcgcgcgc
     4921 gtagtcgctg cgctaccctc agcacgtgcc tgcgcgcgtg ctgatcgtcg tgctcttgct
     4981 cgcgtcccga gcacgggcag aatcagtcgc gccgccgccg gtcacgtctg gtgtgcagct
     5041 cgtccgcgcg gacgcgcaat ggcgctatca gatattcacg gcgccccagc tcgcgccgca
     5101 gatcggcgcg ctcgcgatca gcggcctcga tgtcgcactg gggcgcgcgc aatcgccgat
     5161 cgcagtgctc ggcgacgagg ccgatgcacc cgtcgcgtgg ccctacgacg tcgatgcggc
     5221 gacggtcgcg aacggcccgc ttgcgccgac cgccagtgat gcgcgcatcg cggcgctgtt
     5281 cgcggtgacc ccgttcacgc tcacgcacga gcaccaaggg ctgcgcgtgc tcgagatccg
     5341 gctgcgctac cacgatggct gcgcggtgtg gctcaacggc tccgaggtcg cgcggcgttc
     5401 gctcgacggc ggcaccgtgg cattggccgc gcggccgcac ggtcccgaat gggagacgtt
     5461 ttacgtgtcg gtcgcgccag gcctgctccg gctcggcgag aacgtgctcg cgatcgaggt
     5521 gcatccgtcg gggcgtagca acgcaccgga tcttgcggcg gatgtggtgg gccgccgcga
     5581 gctcggcctc gtgcgtggac cgatcatcac cgacatcggc gcgacgtccg cgacgatcag
     5641 catcgagacc gacgcgaacc tcgacgcagc gctcgagtgg ggcacggggc agacgctcga
     5701 tcatcggctg acgagcgcgc cggggcgcat gcatcgcttc aagctcgacg ggttgccgcc
     5761 gcgcaccgcg atcagctatc gcgtccacgc gggcgcgtcg caatcgccgc agttcgcgtt
     5821 tcacaccacg ccgcctgctg gtgagctgat ccggatcggc atctacggcg acgtgcgggg
     5881 cggtcacgac acgcacagaa agctcgtcga gcagatgctc ggcgaagggc tcgatctcgt
     5941 cgcggtcacc ggcgacatgg tgatgcgtgg caccgacaat ggcgactggc agaagttctt
     6001 cgcggtgacg cgcgagctac tcgcgaccgt gcgttatctg ccggcgatcg gcaatcacga
     6061 tctcggttgg cgccgcgcgg atccagatgt gttcgcgttc ccgacggcgc cggcgggtcg
     6121 gcccgacgcg acatactggt acagcctcga tgttgcggac atccacctcg tgttcctcga
     6181 ctcgaatgcc tacgaacgga cggagcagga gcagtggctc gacgccgatc tcgccgccgc
     6241 gcgtgctcgt ggcgcgcgtg cgatcatcgc gctgacgcac gacgggccgt actcgcgcgg
     6301 tgtgcatcgc ggcagccaga tcgcgcgcga gcgctacgtg ccgattctcg cgaagtatca
     6361 cgtcgacttg ctcgtcggcg ggcacgacca cctctatcag cgcggcgaag cgggcggcat
     6421 tcactacatc gtgagtggag gcggcggggc agggctgtat caaccgacgt gtggcgtcgg
     6481 ccgcaagccg aagtgcgccg tggacggcat gcagaaggtc gcgagcgagc atcactacct
     6541 cgtgctcgcg atcgacaagg agacgatcga gatgtgcccg cgccgccccg acggcaagtt
     6601 gctcgagaaa tgcacgcggt acggtttgtg gcggccgtga gctgagcgca gctgatacgc
     6661 tgccgagatg cgttttgtcc tcgtcgcgct cgcggttgtc gggtgccatc acaatccggg
     6721 cacgcccggt ccttcgacgc cagccaacaa ggctgcggcg cccgattacc gcacgacggc
     6781 cgacgacgag ctcggctttc tgcccgtcgg caccgacatc gtgatcggga tgaacgtcac
     6841 gcaatttcgc gggaccgcga tgtggaacag cttgcagccg cagatcgacg gtttccgccg
     6901 cgagctcgag cagatcgtcg gtgactgttc gcacccgaac ccgatcgaca cgatcgagcg
     6961 cggcacggtg gcgatcaacc tcgcaccgga cggcaatccg cacggcgtgt tcgtcgtccg
     7021 cggtatcgat acgacacgcg tgctcgactg catgatccag cagatgaaga aggagggcgg
     7081 taccgggatg ctcgatcgtg gcgtcgcgat catgtcgatg ccgggcagcc gcacgcagtt
     7141 ggcggcggcg gtcgtcgggc cgtcgacgct cgtcatgcag accgagacga ccgtgaccta
     7201 cgactcgatg acgaagatcc tcacgagcgg ctcgccgctg cgctcgtcgc cggtcttcat
     7261 ggagttctac aagcggcggg aggcaggtgc gtcgttctgg ggcatggcga acggcaacgc
     7321 gtggttcttc gaagagctcg cgcagatggg aatgcggccg aagtcgctgg acggcacgct
     7381 catcgtcacc gatcgcctcg cgttttcgat ccgcatgacg atggcgtcgc cggcggacgc
     7441 cgccaaggtc agcgccgagc tcgacaagat caaagggccg gccggcggct tcgtccagca
     7501 gttcgatacg cgcatcgacg acgcgacgct caggattcac gtcgtgatga ccgagcagca
     7561 ggtccgcggt ctcgcgggca tgctcggcgg gatgccctag ctactctttt ccaccgtcct
     7621 cgggcagcgg caccttcagt gtcgcgagcg tcttgcgcac gatgctccac accgcgccgg
     7681 catcggtggt gacatcgtcc cagcgcccgg ggaagcggcc gcagaacgat gtcttcaaga
     7741 tctcgggaag tgcgcgcttg ccgtcgtcgg cgaacagcgg gtgctcgagg tagacgcacg
     7801 cctcgtcggt cgcgcgcacg gacaggctgc ccggtagatc ggcggcgctg cggatcgcgt
     7861 cggcaacgcc gacgatgatc tcgaggttcg cggccgagag ctccgggtgg cgcttcgcga
     7921 ggatttcgac ctcggccttc ggcggcggat aatccatctg cagcggcgcg aagcgatcga
     7981 gctgcgcggc atcgatgccg aacgtgccgg tgaactcgcg gccgcgattg acggcggcga
     8041 tgaactgcac gttgtcggga atcggaatga acgtgttctc gatcgggtga tagacgcggc
     8101 gcgtggcgtc gagcgccggc atcagcgcgt tgcgcgcgct ttcctggcag cggttgagct
     8161 cgtcgaggaa cacgaggtac ttgtgctcgg gatgctcggc ggcctcctcg agcgcgacga
     8221 gcacgtcggt cttgatgaag tcggtgaccg gtgcgcctga tgccgacgcg cggacctgga
     8281 tgctgacgaa gaagtcgctc gcttcgacga ccgcgccgca gttgaagaac acgaacacca
     8341 taccgagact gtcggcgatc gcgcgcgcga gcacggtctt gccgcagccc tgcggaccgt
     8401 cgagaagaat gttgagcccg ctctgcagca gcacttgaag tttcttcgac acgatcggat
     8461 cgaggtacat gccctcgatg cggaatggtg cgcgcgcgac gaactcgacc tcgatgcggc
     8521 cgcgatcgtc acgctcgggc ttgtgcaccg cgaggacgcg aacgaggcac gggacgccgg
     8581 gcgtgatgcg gtcgtcgttc gacagcacga ccttcggagc gcggcggcca tcgaggtgcg
     8641 tcgcgcgcag cttgaacgtc gagccctcgg cagggttcgg ccagaagatc gcttcgaccg
     8701 tgcggccagg agcgagctct tcgactgtcc atttactcgg catcggatgt ccttcttacg
     8761 acgacaacga ccggcgcgcc agctcgccaa tcatgcgacc gaacttcgcg accgcgatgt
     8821 cgggctggtt gtcgtcgagc acgatgtagt gcgggaagca cacctcgtcg agcgcgcgta
     8881 ccgcgacctg cgcgcacacg atgttgcgtc ggcgcgtgag cgtggtgacg aggccgcgca
     8941 gcgccgtgac cgagcactcg gtcggcaggc cgtcgctgat catgacaagc accttcgcgc
     9001 gttgcttcgc gttgagcgcg acgttggctg cgtgatagag cgcggccgcg tcgttgttgc
     9061 caccgctcgc gtgcaggccg accacgccac aatcgttcgc gctgccggca tcgtagatca
     9121 cgctgtcggt gaagccgaag aagcgcgcct cgacgccggg cagggtgcgg acggcttccg
     9181 agacgagcac cgcgaagcgt ttcgcgcgtt cgatgttctg gccggcggtc atcgagctcg
     9241 agcaatcgac gagcgtgccg aggaagaggt cggtgcggcg ctgcgggttg cgcgcgatca
     9301 ggatgcgcgg atcgttgcga gtgacgagtg gggtgagccg ggtcttgtcg agtgcacgac
     9361 cctgcgtgcg ggcgtgctgc ggcacccagc gcaggccgag ctcgtcgagg aactggcgga
     9421 ggcgatgtgc gtggcgtgac acgtcggtcg cgagcttgcg atggacgtcc gcgtcgccgc
     9481 gcacgcgcac gatgcggttg atcggattga acttgatgtc ggggctgacg ttgagctgga
     9541 ggcggctgcc gggctgcggt gggccgcgct tggaacggcg gggatcgagg atgcgctcga
     9601 cctcgcgctg cagaatgtcg tcgtcgacac ccgcaccgaa tacgtcgtcc tcgcggtcgc
     9661 cgaagatgag gccttcgggg ccgccgaaca ccttcgcgat ctggtgcttg ccgccaaaca
     9721 gctcgacgag ctgattcgtc aagttgtaga ggcccttcat gtcgagcccg cgcagatcct
     9781 tgccgcacag cgcgagtgcc ttctcgatca acggatcgcc ggcgcggttg ccgaggccca
     9841 tgcgcagcgc gcgagagaac cgcgcaaccg gatggcctcc gcgatcgagc tcgccgagga
     9901 tcgcgccgcg gcgtacgcgc accgattcct cgtcgaacgc gacctcgagc tcgccattcg
     9961 tgaacgcgcg cgcggtcgac gcgccgagtg cgtcgaacag cacggccacc ttcatctcct
    10021 gcggcgcgtg ctggaacgcg taggcgccga ggcgcttcaa ccgatcgccg tactcggggt
    10081 cgagcgcgcg caggttgcgc tcgagatgct cgtcggcgat caggttcagg aaatggccga
    10141 tgccttcctg atgcgcctgc ttccacagcg cctgcgcttc ctcgctgcgg tgatacacgt
    10201 gatgaccgag ctcgtgcagc acgaggcctt cgacgatgtc ctggccatac gtctcgccgc
    10261 gcagcatcgg cagcggtgac acgaagatgt ggttgccgtc gagcttggtg tggccgaggt
    10321 ccttctcctt cgcggtcatg tggatgcgga acaaccggcc ggtgagctcg acgccgcgac
    10381 ggatgcccca cgcgaatacg tcggcgacgc ggcggaggcg ggcatcaccc gcgctcgccg
    10441 tcaccgcgta cttcgaagcg gcgcggcgcg tcgccgtgag cgcggcctgc tcgaagatgc
    10501 gcagatcgat gtcggtctgc gtcgcgtgat cgaggaagcc ctcgtcgcgc atgcgctgca
    10561 cgatcgtcca catccgcttc tccgagcaca cgagcgggcc gccgacgagc gcggctgaca
    10621 cgagcatctc cgcgaccgcc gccatatgct cgcggggcac gagctcgagc gtgtacagcc
    10681 agtcgtcggc acgctcgtcg gcgatgtact cggcgagcag cggcaggccg accggatcgc
    10741 caaagtgttg cgcgagaaag cgcgcgacgg agacgcgcag gtggagcggc cggtcatcgc
    10801 cgacttcgag gaagcgcgtg agaccgtcct gcacggtcca ggtcggacgc gtgagcgaga
    10861 gcagcgtgtt gatcgcgcgt tgataggcgt ggtggtgcgg cgagtcgcat aggccgatcg
    10921 aaatcgcgac gacgtgaccg accttgacaa gcgcgtccca atcctcgcgc ctgaagtacc
    10981 agcccttcgg cggcgcgcgc acggccgcga ctgcgcgcaa cagcgcgtcg ccttcaccgc
    11041 gctgggcgag cgcgagcgag atatggaact gccacgctgg cggcagatcg ggctttgtcg
    11101 acaacgcgcg taccgcggcc agcgcgggcg cgtgatccca gagcgggatc gtcgcgagca
    11161 atggaatcgg cgccgcgagc agctcgagct ccgcgagcag gccggcgagg cgcgcttgac
    11221 catcggcgcc gagcacgacg agggcgagtg ccgcctcctg cacgatcgcg ggccgcggat
    11281 cggcgcacca ccgcgtcagc gcatcgaggt ccgtcgacgc gcggatctgc tcgagcagcg
    11341 aggacgacgg cagctgtgcc gtgcgcggcg gatccgggat gccgtcgagc cggacgagac
    11401 gcgcaaggta ctcgcgcgct tcggcgtcgg cgtcggcgat gcggtcgagc atgcgatcgc
    11461 ggatctcgac gagcgcgatc gtcttgcttt cgacgagcgc gacgagcaac cgcgcagcgt
    11521 ggcggccgat gtcacggtcg atcgcctcgg cgcagtactg cccgagcgtg atcgatgcga
    11581 ggtgcttgaa gcgatccggc gtgcggtagc ggatcagcat catcgcctcg gaaatcgcct
    11641 tccacagcgt gtgccgcgcg agccgccccg gcagctcctc ggcgacctgc agcgcgtgat
    11701 acgggccgcc gaccgcgtcg atccaatcga gcagcgcgaa cgagtgcgct tcccaccgcc
    11761 acagccgcgc atgggcgagc accggcagct ccttgacgcg aacccaccgc acggccgcgt
    11821 tgtcgaggtc ggcgtcgaac tgcggcgtcg gctcggcaaa ccgatcgagc gtgcgcgcga
    11881 cgtcctgaag cggatccgcg cagccgatca gcgcgttgca cgctgcgacg ctcgagcccg
    11941 gcgagcgctc gctgagcgcg gcgcaaacgc cgctgatgtg gccgt
//
LOCUS       DASUFX010000735         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_535986,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000735 DASUFX010000000
VERSION     DASUFX010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535986"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFV99_29700"
     CDS             <1..1038
                     /locus_tag="VFV99_29700"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5063603.1"
                     /translation="ARAVASVDSLTQPAAPSRTRFYRDTIFRRVRSGAATAFFGGLSS
                     IARVHPMSKPERHGVEIENDLIYGPHGDWNTLDIYRPIHRPKPWPVVFYVHGGAFHLL
                     SKDTHWLMGLVFARFGYLVVNISYRLAPKHPYPAAIEDTCAAYRWLAANIHELGGDPN
                     RVAVAGESAGGNLITALTLAACQPRPEAWAREVYDTGLVPRATMPFCAILEVSRPERF
                     SQRKPNLPRWVDGMLRDAAASYLHGHSYEPSVETELANPVRVLEDAAGVTHESRFPRR
                     LPPFFAPVGTRDPLLDDTRRLEAALRTLEVPVEARYYPGGIHAFHALVWDPAARRCWR
                     DALAFLDRHMR"
     gene            1045..2418
                     /locus_tag="VFV99_29705"
     CDS             1045..2418
                     /locus_tag="VFV99_29705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5063604.1"
                     /translation="MTPPKRQLGLGITAAIVVANMIGTGVFTGTGFNANALHDPKTIL
                     LAWVIGGVVALCGAACYAELGTLMPKAGGEYVYLREAYHPALGSMSGWVSLTAGFSGA
                     IATSALLFSKYLGTLVGGIGSPVAQKTIAIALIAVITAMHSFDTKVGGRVQQIFTILK
                     VALIVVFIAAGFLVGDGSWSHFETQAGGFSNIATTAFGIQLMYISFAYSGWNAAAYIA
                     GEVDKPEKTLPRSLLLGTGAVMALYVLLNVIYFYALPSSVLAGPADTFAPVVEVGHAS
                     AVQLFGASAGNLITSLIALALVSAVSAMVMAGPRVYAAMAADRALPHQLAYYSKRGVP
                     VAAVAAQGVLATMFVIVGEIGELMRFVGFTLAIFAALAVGALFILRARGLRGAYRTFG
                     YPVTPIIFIAVSGWIAYAQIKNNPKESAVVALGLAAIALVYVLLPKPQPSPPEPQKLP
                     EARVVDE"
     gene            2431..3279
                     /locus_tag="VFV99_29710"
     CDS             2431..3279
                     /locus_tag="VFV99_29710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063605.1"
                     /translation="MRRHVVLLMLAATSGALATPAPQPVTWADWVGDWDGKLKWANCS
                     LDGEDSASFPLEALDGTVSLDLTPAAKQFPQLSLVEDGEGWAGQQNDVSVHVKRGKAD
                     KSGDKLELAIDLDSGCSVRGTLTRASVGIAACDRLAAWARIETHCTKLSRPPLENTAR
                     LARQKAEWRKARGDDRTKLSAQCTARSTKVEQELVDAGCAPHPDPAIGMHGAECQALR
                     GIAGRLNRCGNLPSDQRDAYAREVLVLLAAAQGADKASLPVVDGECKRERDKLFAIAQ
                     QVGCPP"
     gene            3358..4686
                     /locus_tag="VFV99_29715"
     CDS             3358..4686
                     /locus_tag="VFV99_29715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063606.1"
                     /translation="MTTTFCDPAQVVNRKSPGKLDDWMRALIAFACCVTFLGVAIGSH
                     LLFGQIDRAVERGWVPSGIVFLTAFSVLAAYLPPAKLSRSLRVAVLLPVVHASILALA
                     WPAWLVLSRFLNDHNSATELVTHFPIATAAGITLVAFIAFVVVVARRRSGEWLHGLTM
                     LALSELLLLGLWLPISCAICSGGRFYWTPTHPLVDDLLGRVLLTIVPPTVIALTFAAA
                     GVIQPARLVAARRRIVAVVGTLFVLAVATRLGASAREMVLYSNLLPMLLVALCVAIAS
                     LMLFAAAFAIRSLRAWRAFRRMRRTDGVVADDDKAPALGFEITSWLRGPRIVQRPFAV
                     TIAGATIPVSGAHLVAALPAATTQLNVGECYGVLRAGDRVTIAGHANASGDPFRTSAA
                     PLSGELFIAPADRGPSGFAAVALAMWRPCVAYLLIVVAVAIPALAALAAT"
     gene            4705..>5467
                     /locus_tag="VFV99_29720"
     CDS             4705..>5467
                     /locus_tag="VFV99_29720"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX5063607.1"
                     /translation="MTRFGIGAALMAAFAVTNAADAGPNLYVSAGEQPLVDDATQVVI
                     MRKGTRTVVSVMPSYHGPDDPFAMVFPVPAAVQASDIKVVDPLLFNAVDRLAAPRLVE
                     LWEQDPCRSPADDAKGRANKASATDADKAGNKSVSDPGVTVDARALAGAYQIVVLSAK
                     DAAGLDAWLKREKLQLPAGADALLESYIDRGMKLVVAKVDPKRVKLDGEHVVLPPLRV
                     HYDSEQLTLPIRVGLANSSGTQDLVINILSPRQRFA"
BASE COUNT          817 a         1784 c         1839 g         1027 t
ORIGIN      
        1 gcccgcgcag tcgcctcggt tgattcgctt acacagcctg ctgcgccgtc acggactcgc
       61 ttctatcgcg acaccatctt tcgccgcgtc cgctcgggtg cggcgactgc gtttttcggc
      121 gggctgtcgt cgatcgcgcg cgtgcatccg atgagcaagc cggagcgcca cggcgtcgag
      181 atcgagaacg acctcatcta tggcccgcac ggcgactgga acacgctcga catctatcgg
      241 ccgattcatc ggcccaagcc gtggcccgtc gtgttctacg tgcacggtgg tgcgtttcat
      301 ctgctgtcga aggacacgca ctggctgatg ggcctcgtgt tcgcccggtt tggctatctc
      361 gtcgtcaaca tcagctaccg gctcgcgccg aagcatccgt atccggcggc gatcgaggac
      421 acgtgcgccg cgtaccgctg gctcgcggcg aacattcacg agctcggcgg cgatccgaat
      481 cgcgtcgcag ttgccggcga gtcggcgggc ggcaacctga tcaccgcgct cacgcttgcc
      541 gcgtgtcagc cgcgtcccga ggcatgggcg cgcgaggtgt acgacaccgg gctcgtgccg
      601 cgggccacga tgccgttctg cgcgattctc gaagtgtctc ggcccgagag gttctcgcag
      661 cgcaaaccga acctgccgcg gtgggtggat ggaatgctgc gcgatgccgc cgcgtcgtac
      721 ctccatggtc attcctacga gccgagcgtc gagaccgagc tcgcgaatcc ggtgcgcgtg
      781 ctcgaggatg cggcaggcgt cacgcacgag agccgcttcc cgcgccggct gccgccattt
      841 ttcgcacccg tcggcacgcg cgatccgctg ctcgacgata cgcgccggct cgaagccgca
      901 ttacgcacgc tcgaggtgcc ggttgaagcg cgttactacc caggcgggat tcatgcgttt
      961 cacgctttgg tttgggaccc agctgcgcga cgctgttggc gcgatgcact cgcattcctc
     1021 gaccgtcaca tgcgctagga cctcgtgacg ccaccgaagc gacagctcgg gctcggcatc
     1081 accgcggcga tcgtcgtcgc gaacatgatc ggcaccggtg tcttcaccgg caccggcttc
     1141 aacgcgaacg cgctgcacga tccgaaaacg atcttgctcg cgtgggtgat cggcggcgtc
     1201 gtcgcgctgt gcggtgccgc ttgctacgcc gagctcggca cgctgatgcc gaaggccggc
     1261 ggcgagtatg tctacttgcg cgaggcgtat cacccggcgc tcgggtcgat gagcggctgg
     1321 gtatcgctga ccgccggctt ctccggcgcc atcgcgacca gcgcgctgct gttctcgaag
     1381 tatctcggga cgctcgtcgg cggcatcggt tctcccgtcg cacagaagac catcgcgatc
     1441 gccttgatcg cggtgatcac cgcgatgcac tcattcgaca ccaaggttgg cggccgcgta
     1501 cagcagatct tcacgatcct gaaggtggcg ctgatcgtcg tgttcatcgc cgcgggcttt
     1561 ctcgtcggcg acggcagctg gtctcacttc gagacccaag caggtggctt ttcgaacatc
     1621 gcgacgaccg cgtttgggat ccagctgatg tacatctcgt tcgcgtactc gggctggaac
     1681 gctgcggcgt acatcgcggg cgaagtcgat aagcccgaga agacgttgcc gcgctcgctg
     1741 ctgctcggca ccggcgcggt aatggctctc tacgtgctgc tcaacgtgat ctacttctac
     1801 gcgctgccgt cgagcgtgct cgccggcccg gccgacacgt tcgcgccggt cgtggaggtc
     1861 ggtcatgcgt cggcggtgca gctgttcggc gcgagtgcgg ggaatctcat cacgtcgctg
     1921 atcgcgctcg cactcgtctc cgccgtgagc gcgatggtga tggcgggccc gcgcgtctat
     1981 gccgcgatgg cagcggatcg tgcgctgccg caccagctcg cgtactactc gaagcgcggc
     2041 gtgcccgtgg ccgccgtcgc ggcgcagggc gtgctcgcga cgatgttcgt gatcgtgggt
     2101 gagatcggcg agctcatgcg gtttgtcggt ttcacgctcg cgatcttcgc ggcactcgcg
     2161 gtcggtgcgc tgttcatcct gcgcgcacgc ggattgcgcg gcgcataccg tacgttcggc
     2221 taccccgtga cgccgatcat cttcatcgca gtctcgggct ggatcgcgta tgcgcagatc
     2281 aagaacaatc cgaaagagag cgccgtcgtc gcactcgggc tggctgcgat cgcgctcgtg
     2341 tacgtgctcc tcccgaagcc gcagccttcg ccgccggagc cccagaaact cccggaagcg
     2401 cgcgtcgtcg acgagtgata gactgcaagc gtgcgccggc acgttgtact gctgatgcta
     2461 gctgcgacaa gcggagcgct cgcgacgccg gctccgcagc cggtgacctg ggcggactgg
     2521 gtcggcgact gggacggcaa gctcaagtgg gcgaactgct cactcgatgg tgaggacagc
     2581 gcgagctttc cactcgaagc gctcgacggc acggtgtcgc tcgatctcac gcccgctgcg
     2641 aagcagtttc cgcagctctc gctcgtcgag gacggcgagg ggtgggcagg gcagcagaac
     2701 gacgtcagcg tgcatgtgaa gcgcggcaag gcggataagt cgggggacaa gctcgagctc
     2761 gcgatcgatc tcgacagcgg atgttcagtg cgcggcaccc tgacgcgcgc gtcggtcggc
     2821 atcgcggcgt gcgatcgact cgccgcgtgg gcgcggatcg aaacgcattg cacgaagttg
     2881 tcgcgtccgc cactcgagaa tacggcgcga ctcgcgcgcc agaaggccga gtggcgcaag
     2941 gcgcgcggag atgacagaac caagctgtcg gcgcagtgca ctgcgcgcag cacgaaggtc
     3001 gagcaggagc tggtcgacgc gggctgtgcg ccacatcccg atcccgcgat cggtatgcac
     3061 ggtgcggagt gccaggccct gcgcggcatc gccggccggc tcaaccgttg cggcaatctg
     3121 ccgtcggatc aacgcgatgc gtacgcccgc gaagtactcg tgctgctcgc ggccgcgcaa
     3181 ggtgccgaca aggcgtcgct tcccgtcgtc gatggcgagt gcaaacgaga acgtgacaag
     3241 ttgtttgcga tcgcacagca ggtcggctgc ccgccgtaac gggcttcggg cctcgggctt
     3301 cggacttcgg ccagaatcct cgaagcaact tttgtcgtcg cgcgttgtca ttccactgtg
     3361 acgacgacgt tctgtgatcc ggcgcaggtg gtgaatcgga agagccccgg caagctcgac
     3421 gattggatgc gtgcgctgat cgcctttgca tgctgcgtga cgttcctcgg cgtcgcgatc
     3481 ggcagccatc tgttgttcgg ccagatcgat cgtgcggtcg agcgtggttg ggtaccgagc
     3541 ggaatcgtgt tcctcaccgc gttctccgtg ctcgcggcgt atctgccacc ggcgaaactg
     3601 tcacgctcgc tgcgggtcgc cgtgctgttg ccggtcgtgc acgcgagcat cctcgccctc
     3661 gcgtggcctg cgtggctcgt gctttcgcgg ttcttgaacg accacaattc ggcgacagag
     3721 ctcgtcacgc actttccgat cgcgacggct gcgggcatca cgctcgtcgc attcatcgcg
     3781 tttgttgtcg ttgtggcgcg ccgtcgcagc ggcgagtggc tgcacggtct cacaatgctt
     3841 gcgctcagcg agctgctgct gctcgggctg tggctgccga tctcgtgcgc gatctgttcc
     3901 ggcggccggt tctactggac gccgacgcac ccgctcgtcg acgatctgct cggtcgcgtg
     3961 ctgctcacta tcgtgccacc aacagtcatc gcgctgacgt tcgcggccgc cggtgtcatc
     4021 cagccggcgc gccttgtcgc cgcgcggcga cgcatcgtgg cggtcgtcgg cacgctgttc
     4081 gttctcgccg tcgcgacgcg gctcggtgcg agtgcgcgcg agatggtgct gtactcgaac
     4141 ctgttgccga tgctgctcgt agcgctgtgc gttgccatcg cgtcgctgat gctgttcgcc
     4201 gccgcgttcg caattcgtag cctgcgtgca tggcgagcat ttcgccggat gcggcgcacc
     4261 gatggcgtgg tcgcagatga cgacaaagca cctgcgctcg gtttcgagat cacgagctgg
     4321 ctgcgcggtc cgcgcatcgt gcagcggccg ttcgcggtca cgatcgcggg cgcgacgatt
     4381 ccggtcagcg gtgcgcatct tgtcgctgca ttgccagcgg caacgacgca gttgaacgtc
     4441 ggcgagtgct acggcgtgct gcgcgctggt gatcgcgtca cgatcgcggg ccacgccaac
     4501 gcaagtggcg atccgttccg cacgtcggcc gcgccgctct ccggcgagct attcatcgcg
     4561 cccgcggacc gcgggccgtc gggatttgcc gcagttgcac tcgcgatgtg gcggccgtgc
     4621 gtcgcgtatc ttttgattgt cgtcgcggta gcgattccgg cgcttgctgc gctcgccgcg
     4681 acctagatcg ggcatgctcg cgggatgacg cgattcggga tcggggcggc gctcatggcg
     4741 gcgtttgccg tcacgaatgc cgcagatgca ggccccaacc tctacgtctc ggccggcgag
     4801 caaccgctcg tcgacgacgc gacgcaggtc gtgatcatgc gcaaggggac gcgcacggtt
     4861 gtctcggtga tgccgagcta ccacgggcca gatgatccgt tcgcgatggt gtttccggtt
     4921 ccggccgccg tgcaggccag cgacatcaag gtcgtcgatc cgctgctgtt caacgccgtc
     4981 gatcggttgg cagcgccgcg cctcgtcgag ctatgggaac aagatccgtg tcgctcgccc
     5041 gcggacgatg cgaagggccg tgcaaataag gcgtcagcga ccgacgcgga caaggctggc
     5101 aacaagtcgg tcagcgaccc cggcgtcacg gtcgacgcga gagcgttagc gggcgcgtac
     5161 cagatcgtag tgctgtcagc gaaagatgcc gctggcctcg atgcgtggct caagcgcgag
     5221 aagctccagt tgcccgccgg cgccgacgcg ctgctcgagt catacatcga tcgcggcatg
     5281 aagctcgtcg tcgcgaaggt cgatccgaag agagtgaagc tcgacggcga gcatgtcgtg
     5341 ctgccgccgc tgcgcgtgca ctacgacagc gagcagctca cgctgccgat tcgcgtcggc
     5401 ctcgcaaaca gcagcggcac gcaggatctc gtcatcaaca ttttatcgcc gcgccagcgc
     5461 ttcgccg
//
LOCUS       DASUFX010000736         7969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1785645, whole genome shotgun sequence.
ACCESSION   DASUFX010000736 DASUFX010000000
VERSION     DASUFX010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785645"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFV99_29725"
     CDS             complement(<1..306)
                     /locus_tag="VFV99_29725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEX5063608.1"
                     /translation="MASHFLREIDEQPGALARLLAHRHDAVHEAAARIRAFDPAWVVI
                     AARGTSDNAARYGQYVFGAFNQLSVALAVPSLFTLYSAPPRLGRALTIGISQSGQSPD
                     "
     gene            418..1752
                     /locus_tag="VFV99_29730"
     CDS             418..1752
                     /locus_tag="VFV99_29730"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEX5063609.1"
                     /translation="MSISTRPLILGICLVAACSPPHNNGGDDDDATGDGGRPTDACPC
                     PDAGASAGCQGAGSDAAQPFGNHARAYHAGTILPSHSQEQLDDAVRDFYDHWKADYLR
                     AGCGTGRYYILSGGGHQTVSEAHGYGMLVMALMAGHDPQAKTIFDGLVHYYQDHPSEI
                     VPSLMSWAQGSSCQNVEGVNSATDGDLDIAYALLLADKQWSSGGAINYRALAGEIIDG
                     IRDGEVDTGTNYVQLGDWAHTDGEYGAATRSSDFMPAHYESFAAITGDTSWRQIRDRS
                     YAIMSTMQNQFAAQTGLLPDFIADAATMPRPVNPGFLEGATDGQYGYNACRDPWRIAT
                     DYVVNGDPRSKTIAQKMTTWIRQRTGNDPDAILPGYSLSGTGNGNYLDLAFVAPFGVA
                     AMVDGGNQSWLDSVWDTTVAHYGDGYYQDTIALLSLITMSGNWWAPEAAPCP"
     gene            1768..3555
                     /locus_tag="VFV99_29735"
     CDS             1768..3555
                     /locus_tag="VFV99_29735"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5063610.1"
                     /translation="MSWLSTLQNAYAIHNVIAHRPELPVYVIIDGADGLEWMDVTDPG
                     DRLKPFHFEIFFGKEAARDAHYRASLDEAARTGRPVCRQLFGFWDLFYPLADEPRSYL
                     YVGQFYRVAPDWESLSAQWRQLTRQEPTSANPDFVHFVRMALAQPILEDELLDAYQQF
                     LANYADYLTTREEGATTQEKIDRLNREALSRLWPIETWVDSAIDPDKFKLTPWRLDGK
                     LADWMKEGLGISRLPTTAMAIMPLDSRTSPLDWVQTLVRNARIQRACIAFAREQPETA
                     ATHLSDYGVSLITSARSSKNPARSRLELREHAQKFQAFVKERFGVRTAVGIGRTLAPG
                     APLHESHREAVLALHMCAHLERGVLFFDEHGARAEFRYTELQSASTSLATAFDRENAT
                     EIKLASDRYVQLVLRYSGERLEVARSQFLATLFQLWSGVLRKSSMQHEARDRFANDLT
                     RRLEEAPSLYQVIEAFKEALGRLSFVASKAWQGTNVMRLEATLQYLRENFADPLPLPV
                     VARKAGFSVPTFSRVFRRATGTSFLPYVRAIRVEHAKNLIVTTPMSIDQIAQACGFQS
                     QHHLIRSFKKATSLTPGAYRKARAAVYEE"
     gene            complement(3543..4445)
                     /locus_tag="VFV99_29740"
     CDS             complement(3543..4445)
                     /locus_tag="VFV99_29740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5063611.1"
                     /translation="MTMAMRIGIDLGGTKIEAAALDDKGTVRTRRRTATPRSYDELLC
                     VLAELVRTVESEVGTVRAVGIGTPGQPSPLTGLMANAENTPLQGQPFARDLETVLGRP
                     SRLANDAACFALSEACDGAGAGARVVVGVIAGTGIGGGIVVDGRLLGACEWGHSPLPW
                     AEPHEADASCTCGRHGCIEALLAGPGLARDHERTTKQRIDGATIAAQARAGDAAANAS
                     IERHADRMARALASIVNLVDPDVIVLGGGVSRIDALYELVPARLSRWVAMCPPRVRVV
                     RNVHGDASGVRGAAWLWPPTSHSS"
     gene            4599..6452
                     /locus_tag="VFV99_29745"
     CDS             4599..6452
                     /locus_tag="VFV99_29745"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX5063612.1"
                     /translation="MVMLIVTGSCSAPQSTATKTVVLGGAGHGGTPAPAMPGKNVPAI
                     KVDTVGYPTSWPKIVVFNVPPAGAVVKDAAGTRVLTIESSRVSERGVDAASQDPVWQV
                     DISDLEKPGTYTIEVGQHVSDPFTIAAHPYERALLAGLKSFYFQRTRTALEERYAVWD
                     GDSYTRAKPSHVHDDVGWDLETYPKKTKRWKMDAGWHDAGNYDMYVPSTAPTAQSLLL
                     AYEWAPAVFTDNQLTIPESGNGIPDILDEAKWGLSWVLSMQEPGGAFRHREAVMESSP
                     ELPADQDRTVRWVAGVSTAATAKAVAVLAMASRVYAEHDDTFAARCALAAKRGWAWML
                     EHPERVIADGKGATQPLWDDEPGNSDAGAKMIAAAEMWRTFRDKRALAMSRDLMTDEA
                     AKPNELLRGAWANVGRWAMWRLATDDKTPEDLRADARGRLLAAAALVREQVEKHDGYR
                     CASELSDYYWAHNSNLMEKTHVLAMASRLDPGEHWYMDAARDQLHWVLGRNPNGFSMV
                     TRVGKSPTRLYHMEWGNREPPPPGYLIGGPNAQEMGFLAPGAPAKALLWDNPQPLRSG
                     LPAHALWHWKQTDLWDSGFEEEESWNNGWWTVTEPDILYSAAFVLALVSVR"
     gene            6449..>7969
                     /locus_tag="VFV99_29750"
     CDS             6449..>7969
                     /locus_tag="VFV99_29750"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX5063613.1"
                     /translation="MSLYSIDDNWEMSSSAVGAWHPCKLPATVLGVLAERGVIADPFL
                     GTNLRELPGCGPPAQNFSNHPMPADSPFAVPWRFRRTFRVPADAGRYLRLRFDGINYR
                     AKVWLNGERLGEELVGAYRGFELDVTERVVRDGDNKLVVEVTPPTPCDLAITWVDWNP
                     SPPDKNMGIWRDVWLVDTGPVALRAPFVTSRIAADGTCAWLAIEGDLVNLSDEPQRAV
                     VHARIAELGRAVNQVVELAPREERAFKLSADDHDALVVALPRLWWPRFMGEPALYELE
                     LDVTTGSNGHSAVSDRDRFAFGIREVTSKLTERGHARFEVNGKPLLVRGAGWAPDLLL
                     RRDRARDFAQLQYLKDMNLNCVRFEGMLERAEVLEWCDREGILVIAGWCCCDCWEKWD
                     KWSEQNYRVAAESLRSQIRRVRRHPSMLTWWYGSDFPPPARVERAYLDVLAEERWPNA
                     WQSSAANKPTELTGPSGLKMEGPYEYVPPSYWLEDTARGGAFGFATEISPGVAIPPI"
BASE COUNT         1349 a         2609 c         2777 g         1234 t
ORIGIN      
        1 gtcgggtgat tgccccgact gcgagatgcc gatcgtcagc gcgcgtccca gccggggtgg
       61 ggcgctgtac agcgtgaaca acgacggcac cgccagcgcg acgctgagct ggttgaacgc
      121 gccgaacacg tactggccgt aacgcgcggc gttgtcgctc gtaccgcgcg ccgcgatcac
      181 gacccacgca ggatcgaacg cacggatgcg cgccgccgct tcatgcaccg cgtcgtgccg
      241 gtgtgccagg agtcgggcga gagccccggg ttgctcgtcg atctcgcgga gaaagtgact
      301 tgccacgacc tagctctgac agacgcaccc ccgggattgg aagcgccgag aaaaagtgtg
      361 catgggtctg acccgaatcg accgcggccg cgctctttac ctgcatagcg tcacgtcatg
      421 agcatctcca cgcgccctct gatcctggga atctgcctgg ttgcggcgtg ttcgccgccc
      481 cacaacaacg gcggggacga cgacgacgct accggcgatg gcggtagacc gaccgacgcg
      541 tgtccgtgtc cagatgcggg cgcttcggcg ggatgccagg gcgcgggctc ggacgcggcg
      601 caaccgtttg gcaaccacgc gcgcgcctat cacgccggca cgatcctgcc gtcgcacagc
      661 caggagcaac tcgacgacgc ggtccgcgat ttctacgatc actggaaggc ggactatctg
      721 cgtgcgggtt gcggcaccgg tcgctactac atcctgagcg gcggtggcca ccagaccgtg
      781 agcgaggcgc acggctacgg gatgctcgtg atggcgctca tggccggcca cgatccgcag
      841 gccaagacga tcttcgacgg cctcgttcac tactaccagg accatccgtc cgagatcgtg
      901 ccgtcactga tgtcgtgggc gcagggctcg tcgtgccaga acgtcgaggg cgtcaacagc
      961 gcgaccgacg gtgatctcga tatcgcgtac gcgctcctcc tcgccgacaa gcagtggagc
     1021 tcgggcggcg cgatcaacta ccgcgcgctc gcgggcgaga tcatcgacgg cattcgcgat
     1081 ggcgaggtcg acacgggcac gaactacgtc cagctcggcg actgggcgca tacggatggc
     1141 gagtacggcg ccgccacgcg ctcgagcgac ttcatgccgg cgcactacga gagcttcgcc
     1201 gcgatcaccg gcgacacgtc gtggcgacag atccgcgatc gcagctacgc gatcatgagc
     1261 accatgcaga accagttcgc ggcgcagacc gggctgcttc ccgatttcat tgccgacgca
     1321 gccaccatgc cgcgtccggt caacccgggc ttcctcgagg gcgccaccga cggacagtac
     1381 gggtacaacg cgtgccgcga tccgtggcgc atcgctaccg actacgtggt gaacggcgat
     1441 ccgcgcagca agaccatcgc gcagaagatg accacgtgga tccggcagcg cacgggcaac
     1501 gatccggacg cgatcctgcc cggctatagc ctcagcggca ccgggaatgg caattacctc
     1561 gacctcgcgt tcgtggcgcc gttcggcgtc gcggcgatgg tggacggtgg caatcagagt
     1621 tggctcgaca gtgtctggga cacgacggtt gcccactacg gcgacggtta ctaccaggac
     1681 acgatcgcgc tcttgtcgct catcaccatg agcggcaact ggtgggcacc ggaggctgca
     1741 ccctgtccct aggagatgga ggatgtcgtg tcgtggctga gcaccctgca gaacgcgtat
     1801 gcgatccaca acgtgatcgc ccaccgaccc gagttgccgg tctacgtcat catcgacggt
     1861 gcggatggcc tcgaatggat ggatgtcacc gaccccggtg atcggctcaa gccgttccac
     1921 ttcgagatct tcttcggcaa agaagccgcg cgcgacgctc actatcgcgc gtcgctcgac
     1981 gaagccgccc gtacggggcg tcccgtgtgc cgacagctgt tcggcttctg ggacctgttt
     2041 tacccgctgg ccgacgagcc gcgatcgtat ctgtatgtcg ggcagttcta tcgcgtggca
     2101 cccgactggg agagcttgtc ggcgcagtgg cggcagctca cgaggcagga gccgaccagc
     2161 gccaatcccg acttcgtgca cttcgtccgc atggcgctgg cgcagccgat cctcgaggac
     2221 gagctgctcg acgcgtacca acagttcctc gccaactacg ccgactacct caccacgcgc
     2281 gaagaaggcg caacgaccca ggagaagatc gatcgcctca accgcgaggc actctcgcgg
     2341 ctgtggccga tcgagacgtg ggtcgacagc gcgatcgatc ccgacaagtt caagctcacg
     2401 ccgtggcggc tcgacggcaa gctcgccgac tggatgaagg aggggctcgg catctcgcgt
     2461 ctgccgacga cggcgatggc gatcatgccg ctcgactcgc ggacctcgcc gctcgattgg
     2521 gtccagacac tggtgcgtaa cgcgcgcatc cagcgggcgt gcatcgcgtt cgcgcgcgag
     2581 cagcccgaga ccgccgcgac acacctctcc gactacggcg tgtcgctcat cacgtcggcg
     2641 cgctcgagca agaacccggc gcggtcgcgt ctcgagctgc gcgagcacgc gcagaagttc
     2701 caggcgttcg tgaaggagcg cttcggcgtg cgcacggcgg ttggcatcgg caggacactt
     2761 gctccgggtg cgccgctgca cgagtcgcac cgagaggctg tcctggcact gcacatgtgc
     2821 gcgcacctgg agcgcggcgt gctgttcttc gacgagcacg gcgcgcgcgc cgagtttcgc
     2881 tatacggagc tgcagagcgc atcgacgtcg ctggcgacgg cgttcgatcg cgagaacgcg
     2941 accgagatca aacttgcgag cgatcgctac gtgcagctgg tgctgcgcta ttcgggcgaa
     3001 cgcctcgagg tcgcgcgcag ccagttcttg gctaccttgt tccagctatg gagcggcgtg
     3061 ctgcgcaagt cctcgatgca gcacgaggct cgcgaccggt tcgccaacga cctcacgcgc
     3121 cggctcgagg aggcgccatc gctctatcaa gtgatcgagg cgttcaaaga agcgctcggg
     3181 cggctgagct tcgtcgcgtc gaaggcctgg cagggcacga acgtcatgcg cctcgaggcg
     3241 acgctacagt acttgcgcga gaacttcgcc gatccgctgc cgctgccggt ggtggcgcgc
     3301 aaggctggct tctcggtgcc gaccttttcg cgcgtgttca ggcgcgcgac ggggacatcg
     3361 ttcttgccgt acgtgcgcgc gatccgcgtc gagcacgcca agaacctgat cgtgacgacg
     3421 ccgatgagca tcgaccagat cgcgcaggcg tgcgggttcc agtcgcagca tcacctgatc
     3481 cgctcgttca agaaggcgac gagcctgaca ccgggcgcct atcgcaaggc gcgagcagcg
     3541 gtctacgaag aatgagacgt cggcggccag agccacgcgg cgccgcggac gccgctggca
     3601 tcgccgtgga cattgcgcac cacgcgcaca cgcggcgggc acatcgcgac ccagcgcgag
     3661 agacgcgccg gcacgagctc gtagagcgcg tcgatgcgcg acacaccacc gccgagcacg
     3721 atcacgtcgg gatcgacgag gttgacgatc gacgcgagcg cgcgcgccat gcgatcggcg
     3781 tgacgctcga tgctggcgtt cgctgcagca tcgcctgcac gcgcctgggc ggcgatggtg
     3841 gcgccgtcga tgcgctgctt ggtggtgcgt tcgtgatcac gggcgagacc cggtccggcg
     3901 agcagcgcct cgatgcagcc gtgacgacca cacgtgcacg acgcgtcggc ctcgtgcggc
     3961 tcggcccacg gcagtggcga gtgaccccac tcgcatgcac cgagcaagcg gccgtcgacg
     4021 acgatgccgc cgccgatacc ggtgccggcg atgacaccga cgacgacgcg cgcaccggca
     4081 cccgcgccgt cgcaggcctc ggagagcgcg aagcacgccg cgtcgttggc gaggcgcgac
     4141 ggccgcccga gaacggtttc gaggtcgcgc gcgaacggct gcccttgcag cggtgtgttc
     4201 tcggcgttcg ccatgaggcc ggtgagcggc gacggttgcc cgggcgtgcc gatgccgacg
     4261 gcgcgcacgg tgcccacctc gctctcgacg gtgcggacga gctcggcgag gacgcaaagg
     4321 agctcgtcgt aggagcgtgg tgtcgcggtg cggcggcggg tccgcaccgt gcccttgtca
     4381 tcgagagccg cagcctcgat cttggtgccg ccgagatcga tcccgattcg catcgccatc
     4441 gtcatgtgcg cagtatgcgg acggcgtgta cacagatcga gggcggcgcg aaagcggcca
     4501 ttgtctcgat aagatcgatc cgtacggcgg agcgcgcgat gcgcaacatc gttcgggtgc
     4561 acgacatcgg acgagctcgc tttaccgcgg tggtggccat ggtgatgttg atcgtcaccg
     4621 gcagctgctc cgcgccgcaa tccacggcga cgaagacggt cgtgctcggt ggagctgggc
     4681 atggcggcac tcccgcgccc gcgatgcccg gcaagaacgt tcctgcgatc aaggtcgaca
     4741 ccgtcggata tccgacgagc tggccgaaga tcgtggtctt caacgtgccg cccgccggcg
     4801 ctgtcgtgaa ggatgccgcc ggcacgcgcg tcctgacgat cgagtcgtcg cgcgtgagcg
     4861 agcgtggcgt cgatgccgca tcgcaggatc cggtgtggca ggtcgacatc agcgatctcg
     4921 agaagcccgg cacgtacacg atcgaggtcg gccagcatgt gagcgatccg ttcacgatcg
     4981 cggcgcatcc gtacgagcgc gcgctgctcg ccggcctcaa gagcttctac tttcagcgca
     5041 cgcggaccgc gctcgaggag aggtacgcgg tgtgggacgg cgacagctac acgcgcgcca
     5101 agccgtcgca cgtgcacgac gatgtcggct gggatctcga gacgtatccg aagaagacca
     5161 aacgctggaa gatggatgcc ggctggcacg acgcgggcaa ttacgacatg tacgtgccct
     5221 cgacggcgcc gacggcgcaa tcgctgttgc tcgcgtacga gtgggcgccg gccgtgttca
     5281 ccgacaatca gctaaccatc cccgagagcg gcaacggaat ccccgacatc ctcgacgagg
     5341 cgaagtgggg cctgtcctgg gtgctctcga tgcaggagcc gggcggcgcg tttcgtcacc
     5401 gcgaggcggt gatggagtcg tcgcccgagc tgccggctga tcaggaccgc acggtgcgct
     5461 gggtcgctgg cgtgagcacg gcggcgacgg cgaaggcagt ggcggtgctc gcgatggcgt
     5521 cacgcgtata cgccgagcac gacgacacgt tcgcggcgcg ctgcgcgttg gcggcgaagc
     5581 gcggctgggc gtggatgctc gagcatcccg agcgcgtgat cgccgacggc aagggcgcga
     5641 cgcagccgct ctgggacgac gagccgggca acagtgacgc cggcgcgaag atgatcgctg
     5701 cggccgagat gtggcgcacg ttccgcgaca agcgcgcgct cgcgatgtcg cgcgatctga
     5761 tgaccgacga ggcggccaag cccaacgagc tgttgcgcgg cgcatgggcg aacgtcgggc
     5821 ggtgggccat gtggcggctc gccaccgacg acaagactcc agaagacctg cgcgcggacg
     5881 cgcgcgggcg gctgctcgcg gcggcggcac tcgttcgcga gcaggtcgag aagcatgacg
     5941 gctaccgatg tgcatcggag ctgagtgact actactgggc ccacaactcg aacctgatgg
     6001 agaagacgca cgtgctcgcg atggcgagcc gcctcgatcc cggcgagcac tggtacatgg
     6061 acgccgcgcg cgatcagctg cactgggtgc tcggccgcaa ccccaacggc ttctcgatgg
     6121 tgactcgcgt cggcaagagc ccgacgcgcc tctatcacat ggagtggggc aaccgtgagc
     6181 cgccaccacc cgggtacttg atcggtggac ccaacgcaca ggagatgggc ttcctcgcgc
     6241 ccggtgcgcc tgcgaaggcg ctgctctggg acaacccaca accgctgcgc tcggggttgc
     6301 cggcgcacgc gctgtggcac tggaagcaga cggatctgtg ggacagcggc ttcgaagaag
     6361 aggagtcgtg gaacaacgga tggtggaccg tgaccgagcc cgacatcctg tacagcgcgg
     6421 cgttcgtgct cgcgctcgtg agcgtgcgat gagtctctac tcgatcgacg acaactggga
     6481 gatgagctcg tccgccgttg gagcgtggca tccgtgcaag ctgccagcga cggtgctcgg
     6541 tgtcctcgcc gaacgcgggg tgattgccga tccattcctc gggaccaacc tgcgcgagct
     6601 ccctggatgc gggccgcctg cgcagaactt ctcgaatcac ccgatgcccg cggacagtcc
     6661 gttcgcggtg ccgtggcgct tccggcgcac gttccgcgtg cccgcggatg caggtcgcta
     6721 tttgcgactg cgcttcgacg gcatcaacta ccgcgcaaag gtctggctga atggcgagcg
     6781 gctcggcgag gagcttgtcg gcgcgtatcg cgggttcgag ctcgatgtga ccgagcgggt
     6841 cgtgcgcgac ggcgacaaca agctcgtcgt cgaggtgacg ccgccgacgc cgtgcgatct
     6901 ggcgatcacg tgggtcgact ggaacccgtc gccgcccgac aagaacatgg gaatctggcg
     6961 cgacgtctgg ctcgtcgaca ccgggcccgt cgcgctgcgt gcgccgtttg taacgtcgcg
     7021 catcgcggcc gacggcacgt gtgcgtggct cgcgatcgag ggcgacctcg tcaacctgag
     7081 tgacgagccg cagcgcgcgg tcgtgcacgc gcggatcgcc gagctgggac gcgcagtgaa
     7141 tcaggtcgtc gagctcgcgc cgcgcgagga gcgtgcgttc aagctgagcg ccgatgacca
     7201 cgacgcgctc gtcgtcgcgc tgccgcgctt gtggtggccg cgcttcatgg gcgagccggc
     7261 gctgtacgag ctcgagctcg acgtgacgac cggctcgaac ggacacagcg cggtatcgga
     7321 ccgggaccgc ttcgcgtttg gcatccgcga ggtgacgagc aagctgaccg agcgcggcca
     7381 cgcgcgcttc gaggtcaacg gcaagccgct gctcgtgcgc ggtgctggct gggcgcccga
     7441 tctgctgctc cggcgtgacc gggcgcgaga cttcgcgcaa ctccagtacc tgaaggacat
     7501 gaatttgaac tgcgtgcgct tcgagggaat gctcgagcgc gcggaggtgc tggagtggtg
     7561 cgatcgcgag ggcatcctcg tcatcgccgg ctggtgctgc tgcgactgct gggagaagtg
     7621 ggacaagtgg agcgagcaga actaccgcgt ggcagcggag tcgctgcgta gccagatccg
     7681 ccgcgtccgg cgccacccgt cgatgctgac gtggtggtac ggcagcgact tcccgccgcc
     7741 ggcgcgcgtc gagcgcgcgt atctcgacgt gctcgccgag gagcggtggc cgaacgcgtg
     7801 gcagtcgagc gcggcgaata agccgaccga gctgacgggc ccgagtggac tcaagatgga
     7861 aggcccgtac gagtacgtgc cgcctagcta ctggctcgag gacaccgcgc gcggcggcgc
     7921 gttcgggttc gcgaccgaaa tttcgccggg cgttgccatc ccgccgatc
//
LOCUS       DASUFX010000737        24218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_536308,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000737 DASUFX010000000
VERSION     DASUFX010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24218
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536308"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFV99_29755"
     CDS             <1..1002
                     /locus_tag="VFV99_29755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063614.1"
                     /translation="GHTMLVFRTRGGEASDVPKLPSAVDGLATLSPALADRFATIARG
                     APSDVPVMIRGDTGTGKELVARAVHTLSGRKGPFVAVNCGALPANLVEAELFGHRKGA
                     FTGAGDERTGLVRSADGGTLFLDEIGELPAAAQAALLRVLQEGEVLPIGTDKPLKVNV
                     RLVTATLRDLEAAVTDGKFRSDLLGRLLGLSIALPPLCERREDLALLVQALLDRLAPA
                     RGLRLSPDVVRALYAHTWPRNIRELERALASACALTLERIEIEHLPDAVRASAPPPRL
                     AHAPAQLSDDERKLRDDVAASLLRHAGNVAAVARDLGKDRTQIRRWMRRFNLSRDDD"
     gene            complement(999..2075)
                     /locus_tag="VFV99_29760"
     CDS             complement(999..2075)
                     /locus_tag="VFV99_29760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063615.1"
                     /translation="MKPFRLVEQIGEGASGAIFVGEFGQLRRPAAFKILHPHLVGESA
                     ARRMMAEARALSLLDHPGIVKVFDVDMMQGRVCIVMERLVGEPLSQRLARERLSEERV
                     VLYARQLASALEAAHAVGIVHRDLKPENVFVVPDPDVPFGERVKIIDFGIAKCAEADE
                     DVRTATGIVLGTPTYMAPEQWDQVVDPRADIYSLGVMIYVMATGELPFSGTTDELLAE
                     HAYCAPPRAVDIGGVSSWLSSIIERCLAKRPSERFPTMAALATALRELEQAIASPTRP
                     TPRYAADDSQDEVTRAERRARAVKVRTTAQTLVQTGRRAKLRPITRKSGPPVDRLPRW
                     PVALAFAVLAAVCVNRLAACGLAS"
     gene            complement(2128..5004)
                     /locus_tag="VFV99_29765"
     CDS             complement(2128..5004)
                     /locus_tag="VFV99_29765"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5063616.1"
                     /translation="MLRLLVIFAFLGCTAKAPETRPPDETGPTNPPPPEEPPPPAAAK
                     YVRGSLTPIYQLAPRTEYGRFTESGVTMRDADFTSAQTTFVTAAQKMDEIGAQIAKER
                     ALPLLDLLPRAEDRQRAQQIPFRGNPSDVDIVIVNGRRKAYVPLGGDLMTPGNEVASV
                     DLDTKLVTRIKVGIRPQRIAVHPAGLIFVCNQYSNYISVIDPRTDQVLQGPQGTIEIK
                     TDFYCTDLAFVPRNVAAPDVDEQDLYVANGWRGSVLKYGLTVTRDGISDEPTDVRVTE
                     PAAPNPASQPAAEITGVGANPYRLSLSQDRRALYVANNRGGELARIDLATKTARRIAI
                     NAPVPDVVQANDILIVPTTTIDRGLPDRADPRPTQITANAVQVTGLDGKAHVAHPGAL
                     FDTTKAYNFEDLRNGMLTVDAQLNTGTQPTYFTDDISPEPNFTAQQKVLQGSLPQAVV
                     LNAARTRAFVAMSGSDCVQEFAVGSGLFRLTDAPSQLMRTSERPFALALDETARELLV
                     ATWGGETLEIFDTTTSTLKSRINLGYATIDYPATNIERGEYLFYNTAWSNNGRKSCAT
                     CHFDELLMDGVGFANGATAPTAYHKVPANFNLSTTSSYFWNGSFANGTYTSLAADAQT
                     RTNCELILFGLVEGIASDPNGRVGDPRNRVRSAADAQCRPDTSGNATLPNNFNTITNV
                     IAAQKLVREQVVRDATGLSGFAEVSRDVDFYAVAEMRLPPNPLTYLASRGELASTETA
                     KIEKGKQLFTSAGCANCHDPNNTRHPYTDGLEHGSGTAWTSQFVDTYATDPRIVDSIG
                     AIPQVMLEAISSSTVDKEINVHLDPIDYFAPFCFDVNSCLVFEDPLAVRGNNAAETER
                     LDVLVRINLANADRGFIPGNVRGQPKTNTPSLRGNWFQTNFLRHGHAHTLKEAILAPG
                     HPLLDDGEQGFAVNALGAFDVHGVTSKLTADDFDALYSFVLSIE"
     gene            complement(5004..7142)
                     /locus_tag="VFV99_29770"
     CDS             complement(5004..7142)
                     /locus_tag="VFV99_29770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063617.1"
                     /translation="MTHHLRSLFVVWLAAHAAACNGDINGDDPGATAPECQLDTKSEK
                     TPGYPFDVDTFGSEVMPVIGKSCAAAGCHAPPTGNGTFTVWAAAKPGDCDFAKTFNSV
                     VAKVDLVTPTNSRFIAVISGGSTTHPFTFQPTAPELATLRTFVETASARYAADGGGTT
                     PPPGPSPFDYKVYQSTIQPMLERCTGAACHTNGQGGFTLKAMPSASSADMEANFIAVT
                     SRANLIDPATSVIYTRATVQHGSGTSKTVDSAQAQALLAWIEDAKQNAGSNPNPTCAP
                     INKFNAGVFASEVLPILSGDLDLNAPGGQALGAGCRSTACHGIDRGPGAFSLPLAADT
                     ATLLQNYACFVNLASPAASEILACPLNAPGCRRSPHPGQDVLGGASDLNYQRLLAYIY
                     GANSVTSPLDFAFFARRVNPILNDPNAVEGGAQGRSCSDATSCHGISVAGQTPPNGSD
                     FGVISNASGLERLSFNFVSATSFVNFLNPRGSSLFMYPTNEIANTGGNPFATGIPHPG
                     GVDFAIDSFEAKTILQWAGGLRPDAQGFVRNWLVVGDFPATQIIDPTLIEESSVIPKI
                     FDRGGGAFNRGEWDGLFADATDVDLNTVFPRNATSGRVGYATAYVINTVPRSLRVQLT
                     VSTNNPIRLYVDGGLIAQNVDSGGTSAFFTLGAAGTENAKPVRIMIKILQRETDARFA
                     FTARLRDELGNVLSDATREVVFTLGPNGGI"
     gene            7359..8684
                     /locus_tag="VFV99_29775"
     CDS             7359..8684
                     /locus_tag="VFV99_29775"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063618.1"
                     /translation="MRTRRLNRDRVEIARFRLRGISGPDAPFVVDSETAAVTIGTAQA
                     ELVQLTDPCVSRHHCVIRVGDNGLICEDLGSTNGTFVAGRRITSAVIDPGDTIIVGET
                     VIAIDPLDGTISEPLSHNDHFGPVLGRSAAMRHLFAILERVAPSDATVLIEGETGTGK
                     SMLAEAIHAASPRAKMPFVVVDCASLPHHLLESELFGHERGAFTGAANDRIGMFESAN
                     GGTVLLDEIGELPLDLQPKLLRALERRAIRRIGATQVTPINIRIIAATNRDLRRAVNA
                     HRFRSDLWYRLNTVRLVLPPLRERREDIPSLVASLYRELLADPKAMPPAEVVRPMMRA
                     DWPGNVRELRSAVERSLLGATPSATGSMPDIEEGLPFRHAKNRASSEWERRYLSELML
                     ASGGNISAAARAGQMNRSHLSELLRRHGLVGIADDIAFEPDAEESSPGR"
     gene            complement(8681..12550)
                     /locus_tag="VFV99_29780"
     CDS             complement(8681..12550)
                     /locus_tag="VFV99_29780"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063619.1"
                     /translation="MRNLAARGGAEKLRAINTLRLTGKATMGGGDFSLEAAWGSITTR
                     SGKVRNEITLQGLTQVNAYDGKEGWSVRPFGGRRDAEKASEDQARSFAQDADLDGPLI
                     DWRQKGHKVEYLGTEDVDGTLAAKLRVTRKDGDIQYVFLDPESGLEIRITTVHKVRGT
                     EEIGETDFGGYQQVGGVWIPFSIESGSPGGSRGFRVQVERAEINVPVEDDIFKLPTKA
                     VTRVVVAGPPDKVAPTAAPPLKTGPAVVDAGTISGVGARNIGSAAMSGRIAAVAARNE
                     GGKTILFVGAASGGVWKSIDGGTTFRPVFDKQRVQSIGAITIDPSNPRTIWVGTGESW
                     TRNSVSIGDGIYKSTDEGETWTNLGLPDSERITRILVNPKNSQVVYACVPGKLWSDSN
                     DRGLYKTTDGGKKWSLVLRGANASTGCSSVTMDSKNPDSLIAGMWDFRRKGWTFRSGG
                     DGPDAASASGMFRSSDGGKTWTPINAAKAKGLPATPWGRVEVVMAPSDSKIVYALIES
                     KKSALYRSDDGGKTWDERDKSQMMVWRPFYFARLVVDPTNPNRLFKPDLNLVVSTDGG
                     KSFSYSGGGSHGDWHDVWVDPQNPKHVIGGDDGGLWLSFDGGTRWHKNNSLPVSQFYH
                     VSIDNKDPYQVYGGLQDNSSWVGDSAYGGGITNQRWENLCGGDGFWTIPDPTDPNAVY
                     CESQGGYINRIDRKTMVSRDIQPKAKYKEKLRFNWNTPMAVSPTQKGTIYLGSQFLFR
                     SKDRGNTWQRVSPDLTTNDKEKQKQEQSGGVTVDNSSAEMHTTIYSISESPKNAQVVW
                     VGTDDGNVQISRDGTKTWTNVVGNVPNLPPNSWVSWVEASRFDAGTAFATFDRHTFGD
                     MTPWVYKTTDFGKTWKRIVGPEQKVRGYAHVIKEDAVAKNLLFLGTELGLYISLDGGG
                     TWAEFKGGDFPAVAVRDLAVQPRDNDLVIATHGRGIWIIDDITPLRALTAPTLEKQTV
                     FLPQRPIQQRMQSSGGWVEGDATFVGANPPSGAVITYYLRSRHIYGPIKLEILDDKGK
                     VIDTVTPTKRRGINRVTWTMRVKGPRVPRAAQVAFSASQGPRVVPGTYTVRLTRGADV
                     LETKLAIGLDRRAPYDAASKKAQFVAVMKAHELFNDMSKLVDQIEALRGAVAERAKAL
                     PASDALAGKLRKLDDKLEEARKLVVATTEGGAITGEERIREHLDTLYGAMNGWEGKPT
                     PYQLERLDVLKKELGDVQATLKTVATKDAPALSDELKQKKLQPLPALSQLDMPETLDR
                     VALECLDSMGKQCDEGEDRAAREQERD"
     gene            complement(12718..13866)
                     /gene="mltG"
                     /locus_tag="VFV99_29785"
     CDS             complement(12718..13866)
                     /gene="mltG"
                     /locus_tag="VFV99_29785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5063620.1"
                     /translation="MSKRSFRVALAVVIGSVILVVGIAGWFVHRAFAYPDEAHSGSGK
                     EIEVEIKSGMSFPAVASLLASQHIIERPTWFRLYAMWEGDTTNIKPGKYMIKDNLTPA
                     QVLKIIITGVKEITTRVKLPEGKNMLEYFELIEKACPVGMKAPCPKITSAKELEQLAR
                     DKDFLAGHAIVGDTVEGYLFPDTYEFRVGEKPRVVLERMITRYQEVWQDVLAKHPKDA
                     ARIKDKMRWTDRDILTMASIVEKEAVEPTERPRIAQVFINRMTKPDFKPKRLETDPTI
                     RYGCLVPEKKSTACVAWNEPCAKQNLPPGCDRLHRAQLDDKDNPYNTYQLEGLPPGPI
                     ASPGRSSIEAVVSPDGSDYLYFVAKNDREHAFAKTFDEHKKNVEKYMK"
     gene            complement(13883..14305)
                     /gene="ruvX"
                     /locus_tag="VFV99_29790"
     CDS             complement(13883..14305)
                     /gene="ruvX"
                     /locus_tag="VFV99_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5063621.1"
                     /translation="MRVLGLDVGSKTIGVAITDEVGIAAHPLRVLERKGTDADVSAIQ
                     TLVAEHDIRQIVVGMPFELSGKVGHRAKRVQEFANALQAKLGDAVKLHEQDERFTTAE
                     AERVLLDADLSRQKRKQVIDQQAAALILQAWLDGQRPR"
     gene            14770..15060
                     /locus_tag="VFV99_29795"
     CDS             14770..15060
                     /locus_tag="VFV99_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX5063622.1"
                     /translation="MSDEPAAPPKLQVQIDDDISQGVYSNLVLLNHTENEFVLDFAFI
                     QPSNGRAKVRTRVISSPRHTKRLLLALQKNLERYEERFGRIELGADEEPIVH"
     gene            complement(15242..16186)
                     /locus_tag="VFV99_29800"
     CDS             complement(15242..16186)
                     /locus_tag="VFV99_29800"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX5063623.1"
                     /translation="MAIAARPPKIERGQTLGIVAPGGPVKRAVLERGLAQLGDAFKLR
                     VADSVLAPHPPGVPSYLSASDDVRAAELNAMLADRDVRAILLARGGYGLMRILPRLDV
                     EALRRDPKPIIGFSDATALLAWAYAAGVRGVHGPMVVQLDRLPAEHITHMIDLISVPR
                     APGVRPWQLASHGKGRYRGPLVAGNLTMYSLLVGTPWEVSVRGAIAVIEEVGERPYEL
                     DRYLTQLALTGELAQLAAVVVGDLTRCVDANPPTGVPDPEDAALQTVLERLRAAGTAA
                     AVGAPIGHGDRNEAVPFGADTILDLDAGTIEILDPAVD"
     gene            complement(16205..16891)
                     /locus_tag="VFV99_29805"
     CDS             complement(16205..16891)
                     /locus_tag="VFV99_29805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063624.1"
                     /translation="MSESFVDLSYRGLALGKRIKLTQVRATAGYLEMPTPMPVGTTIG
                     MSTDDGVLFEATVAEVREQVTGSDKPPGMVVKPKLDADAQRSWWKQRVTVDEKPLPKP
                     DAEGKVTVRSRRSTGDATPSELMDDGRNTAVMDAVDDSAGASGELAAQKEVTDPSLPR
                     ISAEIAAQNPIVDDGKRTMMMDAVDLAALGLDPAASSGSMPAASDEDSGATGNGGDKN
                     EKGKKKKKRR"
     gene            16921..17766
                     /locus_tag="VFV99_29810"
     CDS             16921..17766
                     /locus_tag="VFV99_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5063625.1"
                     /translation="MGLIDRLRQLRTNTPADDIDGRTKALVVHGNEFGFDSFGMSKAS
                     LRRAIIVARWLYRHYFRAEAFGIEHLPPSGRMIVVSNHSGQLPFDGLVIASACFLEPP
                     QPRMVRAMVEFFVPTVPFASYLFSRWGQITGTPENCRRLLSAEEAVLVFPEGARGISK
                     PFSKRYQLAEFGKGFMRLALETGSPIVPVAVIGAEEQAPAINVKPLARLLKTPAFPVV
                     PYPPFIAPVPLPVKYRLHFGEPMHFTGDADDDDEILDEKVKQVRNRIQSMIHVGLRER
                     EHVFW"
     gene            17753..18727
                     /locus_tag="VFV99_29815"
     CDS             17753..18727
                     /locus_tag="VFV99_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5063626.1"
                     /translation="MSSGEQQAITDRSIPRPKKVVITGISGRFGHIVARRLHHDLGWS
                     IVGLDRRPMSGRPKDIEHHQVDLRSKKARDMFRQGDVDALVHLGVMHDPRARPAELYS
                     WNITGTTKLLEYCSAYRVPKVVLLSSANVYGPRPDNPQFLTEEAPLLAAQRFPQMRDL
                     VEIDHLVSTFLWRARETETVILRPVHIVGPMHNAPSNYLRIDRPPTLLGFDPMVQLVH
                     AEDVAEAIAMALQPNRRGIYNITGPGEVPLSAVLKELGREPRSIPHPMARPILGLAFR
                     LGFSSFPVAELDFIRYVCMVDGRRAAAELGFRPRFGLRETIHAVDDPV"
     gene            19073..19891
                     /locus_tag="VFV99_29820"
     CDS             19073..19891
                     /locus_tag="VFV99_29820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063627.1"
                     /translation="MKNLSLGLMVAFTTTVLSGCALYFGEDNHNDDTWTYCGSDGYYT
                     CDGDNCQWVGSSCPDQGSGSAQPGGFDCRDNTDCAAGCYCANGTCEEAGFCTQDSDCG
                     TGYVCNEDRSSCEPGGQTCGADADCDAGSVCTEGSCTTTCVCANDAEAAAQGYAYCDE
                     LRITCMSGTDPAGTCGGEPTCNTLPPSCAPGDVPLLGADGCWNGQCEAASDCAVAPSC
                     SHIHDEATCLAQAGCAETVNGINCKKPDGTACMAGSANCTCDSYVFASCRSATP"
     gene            19970..20407
                     /locus_tag="VFV99_29825"
     CDS             19970..20407
                     /locus_tag="VFV99_29825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063628.1"
                     /translation="MGFEFAETMSGTIEWDAEPGKKHPFKFEIRAHAASTREHFANGR
                     AEIRGVISAPPKAASADAEGTIVIRPVGQKIIRYELSFIADDGKRYEMVGQKDIRWSR
                     PLETLTTLPAEILDENHRRVATCLTRFDYRNDLWSFLRSFRPA"
     gene            20441..23578
                     /gene="secA"
                     /locus_tag="VFV99_29830"
     CDS             20441..23578
                     /gene="secA"
                     /locus_tag="VFV99_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5063629.1"
                     /translation="MGLIQKIFGSKNQRELNKLQPNVNRINQLGDSYKAKSDAELRAV
                     TGELKQKLDNGASLDDVLCDAFAAVREASIRTTGMRHYDVQLVGGMVLHQGRIAEMRT
                     GEGKTLVATLPTYLNALEGKGVHVVTVNDYLAKRDAEWMGRIHGFLGLTTGVIVHGLD
                     DFERQRNYNMDITYGQNNEFGFDYLRDNMKTSPDRMVQRGLHYAVVDEVDSILIDEAR
                     TPLIISGSAEASGDLYVKVDRLIPRLKREVDYTVDEKAHSAMLTDDGVEKMEKLLGVD
                     NLFEATNIQLVHHVQQALRAHTLYKRNVNYLVEDSKVVIVDEHTGRKMPGRRWSDGLH
                     QAIEAKEGVEVEEENQTLATVTFQNYFRMYKKLAGMTGTADTEAQEFHQIYKLAVTVI
                     PTNKPIVRKDFPDLVYKNEAGKFRAVAEDIEDCYKRGQPVLVGTVSVEKSEVVANILK
                     AKGLPFNVLNAKQHQREASIVAQAGRKSAITIATNMAGRGTDILLGGNFEAMAKDALQ
                     EEKDKLAQEALAAKPGEGDVPEGDSPYRGSSQPDFDEEKRLKELTEEFKKQCEAEREE
                     VLAAGGLKIVGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLQDDLLRIFGLDRMTG
                     LMERLGLEEDVPIESPMVTRSIENAQKKVEGRNFDQRKNVLEYDDVMNQQRKTIYSLR
                     RQILEGRYYPEPTEDQQKEAFVPEPVTESGDWTQETIRPDVHLVLGKMITELKAKVAE
                     RDEKLARGEPVQDTRPGWRVLRAEIWRQFGTWLDLEKRYSKDDDLLEWAATEVAASQI
                     QQRERLYDLAHTEMANVIEHVLDPEMSEDEWDWNQLDEALTEAFDIPYELSHGTPDEA
                     AAQAWPEIETKLAEREKELTRPWLMYFLRHFYLEEIDQQWIEHLKTMDALREGIGLQG
                     YGQKDPKKEYKKAGFTLFTEMMERIQENVIKKVFHVQIKREEESIPALQAEKQRQMVE
                     RGVADKADEEVDAEVAAKAARRSGKARTSGDGPTTAPIKRERPKVGRNDPCPCGSGKK
                     YKKCHGKDEEASAEG"
     gene            23635..>24218
                     /locus_tag="VFV99_29835"
     CDS             23635..>24218
                     /locus_tag="VFV99_29835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063630.1"
                     /translation="MNNKLWILFLLGACGGGDGAGGLAVDVENPSAFCRAVAETGCST
                     MYACLTDAERMAKHLPMTEAECERQLESGCEDAVDSCNDNTHGYASDAAGACINEMQA
                     ATCNDAGEPWLDAPSCSNICAVTKGSFEVQWAFSPSYHSCSELGVTTVSVYSIAAGGR
                     TYVDTYDCYTGSGITDVLPVGTYSVHLELFNASN"
BASE COUNT         4142 a         8000 c         7798 g         4278 t
ORIGIN      
        1 ggccacacga tgctcgtgtt ccgcacgcgc ggcggggaag cgagcgacgt ccccaagctg
       61 ccgtccgcgg tcgatggact cgccacgctc tcgcctgccc tcgccgaccg attcgcgaca
      121 atcgcgcgtg gtgcaccgtc cgatgtgccc gtgatgatcc gcggcgacac cggcaccggc
      181 aaggagctgg tcgcccgcgc ggtccacacg ctctcgggcc gcaaaggtcc gttcgtcgcg
      241 gtgaactgcg gcgcgctacc ggcgaatctc gtcgaagccg agctattcgg ccaccgcaag
      301 ggcgcgttca ccggcgcggg tgacgagcgg accggcctcg tgcgcagcgc cgacggtggc
      361 acgttgttcc tcgacgagat cggcgagcta cctgccgccg cgcaagccgc gctgcttcgc
      421 gtgctgcaag aaggcgaagt gctgccgatc ggcaccgaca agccgctcaa ggtcaacgtt
      481 cgccttgtca cggcaaccct gcgcgacctc gaggcagcgg tcaccgacgg caagttccgc
      541 agcgatctgc tcggtcgctt gctcggcctc tcgatcgcct tgccgccgct gtgcgaacga
      601 cgagaggatc tcgcgctcct cgtccaggcg ctgctcgatc gcctcgcacc ggcgcgtggt
      661 cttcgcctgt cgccggacgt ggtgcgcgcg ctgtatgcgc acacgtggcc gcgcaacatt
      721 cgcgagctcg aacgcgcgct cgcatcggca tgcgcgctca cgctcgaacg gatcgagatc
      781 gagcatctgc ccgatgccgt acgtgcatcg gcaccaccgc cgcgcctcgc gcacgcgcct
      841 gcgcagctct ccgacgacga acgcaagctt cgcgatgacg tcgccgcgtc cctgctgcgt
      901 cacgccggca acgtcgccgc tgtcgcacgc gacctcggca aggaccgcac gcagatccgg
      961 cgctggatgc gccgcttcaa cctgagccgc gacgacgact agctcgctag tccgcacgcc
     1021 gctaagcggt tcacgcacac cgccgcgagg accgcgaatg cgagcgcgac cggccagcgc
     1081 ggcaatcgat caacaggggg gccggacttt ctggtgatcg gtcgcagctt cgcgcgccgc
     1141 ccggtctgta ccagcgtctg cgcggtggtc cgaaccttca ccgcccgggc tcgccgttcc
     1201 gcgcgcgtga cctcgtcttg ggaatcgtcg gccgcgtaac gaggcgtcgg gcgggtcgga
     1261 gatgcgatcg cctgctcgag ctcgcgcagc gcagtcgcga gcgccgccat ggtcgggaat
     1321 cgctcggacg gccgcttcgc gaggcagcgc tcgatgatcg acgacagcca gctcgacaca
     1381 ccgccgatgt cgactgcacg aggcggtgcg cagtatgcgt gctccgcgag caactcgtcg
     1441 gtcgtgccgg agaatgggag ctcgcccgtc gccatcacgt agatcatgac gccgagcgaa
     1501 tagatatccg cgcgcgggtc gacgacctga tcccactgct ccggcgccat gtacgtcggc
     1561 gtaccgagca cgatgccggt ggcggtgcgc acgtcctcgt cggcttcggc gcacttcgcg
     1621 atcccgaagt cgatgatctt gacgcgctcg ccaaacggaa cgtccggatc gggcacgaca
     1681 aacacgttct cgggcttgag gtcgcgatgc acgataccga ccgcgtgcgc agcctcgagt
     1741 gcagacgcga gctgacgcgc atagagcacg acgcgttcct cgctgagccg ttcgcgagcg
     1801 aggcgctgcg acagcggctc gccgaccagc cgctccatca cgatgcacac gcggccctgc
     1861 atcatgtcga catcgaacac tttgacgatg cctggatgat cgagcagcga cagtgcacgc
     1921 gcttcggcca tcatgcggcg cgcggcgctc tcaccgacga ggtgcgggtg caggatcttg
     1981 aacgctgccg gccggcgaag ttgcccgaac tcgccaacga agatggctcc gctggcccct
     2041 tcgccgatct gctcgacgag ccggaacggt ttcatgcttc aagcttgtcg gaaaccggag
     2101 ccggttttca cacaccgctc cttacaatca ctcgatcgac agcacgaacg agtacagcgc
     2161 gtcgaagtca tccgctgtca gcttcgacgt aacaccgtgg acatcgaacg ccccgagcgc
     2221 gttcacggcg aaaccttgtt ctccatcgtc gagcagtggg tgtccgggtg ccaggatcgc
     2281 ttccttgagc gtgtgggcat gcccgtgccg caggaagttc gtctggaacc agttgccccg
     2341 gagcgacggc gtgttcgtct tcggctggcc gcgaacgttg ccggggatga agccgcgatc
     2401 ggcgttcgcg aggttgatcc gcacgagcac gtcgaggcgt tccgtctcgg ccgcgttgtt
     2461 gccgcgcacc gcgagcggat cctcgaacac gaggcacgaa ttgacgtcga agcagaacgg
     2521 cgcgaagtaa tcgatcggat cgagatggac gttgatctcc ttgtcgaccg tcgagctcga
     2581 gatcgcctcg agcatcacct gcgggattgc accgatcgaa tcgacgatgc gcggatcggt
     2641 cgcatacgtg tccacgaact ggctcgtcca cgcggtgccg gagccgtgct cgaggccgtc
     2701 ggtgtacggg tggcgcgtgt tgttcggatc gtggcagttc gcgcagccag cgctcgtgaa
     2761 cagctgcttg cccttctcga tcttcgcggt ctcggtcgac gccagctcac cgcgcgacgc
     2821 gaggtacgtc agcggattcg gcggcaggcg catctccgcg accgcgtaga agtcgacgtc
     2881 gcgcgagacc tccgcgaaac ccgacagccc ggtcgcgtcg cgcaccacct gttcgcgcac
     2941 gagcttctgt gctgcgatga cgttcgtgat cgtgttgaag ttgttcggca gggtcgcgtt
     3001 gcccgacgtg tcgggccggc actgcgcatc ggccgcactg cgcacgcgat tgcgcggatc
     3061 gccgacgcgc ccattcggat cagaggcgat tccctcgacg aggccgaaca ggatcagctc
     3121 gcaattcgtt cgcgtctgcg cgtcggcggc aagcgacgtg tacgtgccgt tcgcgaacga
     3181 gccgttccag aagtacgagc tcgtagtcga caaattgaag ttcgcaggaa ccttgtgata
     3241 cgcggtcggc gccgtcgcac cgttcgcgaa gccgacgccg tccatcagta gctcgtcgaa
     3301 gtgacacgtc gcgcacgact tgcggccgtt gttcgaccac gcggtgttgt agaacaagta
     3361 ctcgccgcgc tcgatgttgg tcgccggata gtcgatcgtc gcgtagccga ggttgatgcg
     3421 cgatttgaga gtgctcgtcg tcgtgtcgaa gatctcgagc gtctctccgc cccaggtcgc
     3481 gacgagcagc tcgcgcgcgg tttcgtccag cgcgagcgcg aatgggcgct ccgacgtacg
     3541 catcagctgg ctcggtgcat cggtcagccg gaacaaaccg gagccgaccg caaattcctg
     3601 gacgcaatcg gagccggaca tcgcgacgaa cgcacgcgtg cgcgccgcgt tgagcacgac
     3661 cgcctgcggt agcgaacctt gcagcacctt ctgctgcgcc gtgaagttcg gctccggcga
     3721 gatgtcatcg gtgaagtacg tcggctgcgt gcccgtgttg agctgcgcgt cgacggtcag
     3781 catgccgttg cggaggtcct cgaagttgta ggccttggtc gtatcgaaca gtgcgcccgg
     3841 atgggcgacg tgcgctttgc cgtcgagacc ggtgacctgc actgcattcg ccgtgatctg
     3901 ggtcggccgc ggatccgcgc gatccgggag gccgcgatcg atcgtcgtcg tcgggacgat
     3961 caggatgtca ttcgcctgca cgacgtcggg caccggcgcg ttgatcgcga tccgccgcgc
     4021 cgtcttcgtc gcgagatcga tccgcgcgag ctcgccgccg cggttgttcg cgacgtacag
     4081 cgcacgccga tcctggctga gcgacagccg gtacggattc gcgccgaccc cggtgatctc
     4141 cgccgcaggt tggctcgccg ggttcggagc cgccggctcg gtgacacgca cgtcggtcgg
     4201 ctcgtccgag atgccgtcgc gcgtgaccgt cagtccgtac ttcagcacgc taccgcgcca
     4261 gccgttcgcg acgtagaggt cctgctcgtc gacatcgggc gccgcgacgt tgcgcggcac
     4321 gaacgcgagg tccgtgcagt agaagtcggt cttgatctcg atcgtcccct gcggaccttg
     4381 cagcacctga tcggtgcgcg ggtcgatgac cgagatgtag ttcgagtact ggttgcacac
     4441 gaagatcagg cccgccgggt gcaccgcgat gcgttgcggc cgaatgccga ccttgatgcg
     4501 cgtgacgagc ttcgtgtcga ggtcgaccga cgcgacctcg ttgcccggcg tcatcagatc
     4561 gccgccgagc ggcacatacg ccttgcgacg gccgttgacg atcacgatgt cgacgtcgga
     4621 tggattcccg cggaacggaa tctgttgcgc acgctggcgg tcctccgcgc gcggcaacag
     4681 gtccagcaac ggcagtgcgc gctccttcgc gatctgggcg ccgatctcgt ccatcttctg
     4741 ggccgcggtg acgaacgtcg tctgtgcgga cgtgaagtcg gcgtcgcgca tcgtcacgcc
     4801 gctctcggta aaccggccgt actcggtgcg cggtgcgagc tggtagatcg gcgttagcga
     4861 cccgcgcacg tactttgcgg ccgcgggtgg cggtggctcc tccggtggcg gaggattcgt
     4921 cggcccggtc tcgtcgggcg ggcgcgtttc gggtgccttc gcggtgcagc cgaggaacgc
     4981 gaagatcacg agtaagcgca gcattagatg cctccgttcg ggccgagggt gaatacaacc
     5041 tcgcgcgtcg catcgctgag cacgttgcca agctcgtcgc gcagtcgtgc agtaaaggcg
     5101 aaccgtgcgt cggtctcgcg ctgcaggatc ttgatcatga tccgtacggg ctttgcgttt
     5161 tctgtcccag cggcgccgag tgtgaagaat gcgctcgtgc caccgctatc gacgttctgc
     5221 gcgatgagtc cgccgtcgac gtagagccgg atcgggttat tcgtcgacac cgtgagctgg
     5281 acgcgcagcg agcgcggcac ggtgttgatc acgtatgccg tcgcgtaacc gacgcggccg
     5341 ctcgtcgcgt tgcgggggaa tacggtattg agatcgacgt ccgtcgcatc ggcgaacaag
     5401 ccgtcccatt cgccgcgatt gaacgcgccg ccgccgcggt cgaagatctt cgggatcacg
     5461 ctggactctt cgatcagcgt cgggtcgatg atctgcgtcg ccgggaagtc gccgacgaca
     5521 agccagttac gcacgaagcc ctgcgcatcg ggtcgcagcc ctccggccca ctgcagaatc
     5581 gttttcgcct cgaacgaatc gatcgcgaag tcgacaccgc cgggatgcgg gatgccggtt
     5641 gcgaacgggt tgcctccggt attcgcgatc tcgttcgtcg ggtacatgaa caacgagctg
     5701 ccgcgcggat tcaagaagtt cacgaagctc gtcgcggata caaagttgaa cgacaggcgc
     5761 tcgagacccg acgcgttcga gatgacaccg aagtcggagc cattcggcgg tgtctggccg
     5821 gcgaccgaga tgccgtggca gctcgtcgca tccgagcagc tgcgcccctg cgcgcctcct
     5881 tcgaccgcgt tcggatcgtt gaggattgga ttcacccggc gcgcgaagaa cgcgaagtcg
     5941 agcggtgacg tcaccgaatt cgcgccgtag atgtacgcga gcaaacgctg gtagttgagg
     6001 tcgctcgcgc cgccgagcac gtcttgaccg ggatgcggcg agcggcggca gcccggcgca
     6061 ttcagcgggc acgccaggat ctccgacgct gcgggcgacg cgaggttgac gaagcacgcg
     6121 tagttctgca gcagcgtcgc ggtgtctgcg gccagcggca ggctgaaggc gccgggaccg
     6181 cggtcgatgc cgtggcacgc cgtcgagcgg catcccgcgc cgagtgcctg gccacccggt
     6241 gcgttgagat cgagatcgcc cgacaggatc ggcagcacct cgctcgcgaa cacacccgcg
     6301 ttgaatttgt taatcggcgc acaggtcgga ttcgggttgc tgcctgcgtt ctgcttcgcg
     6361 tcctcgatcc atgcgaggag cgcctgtgct tgcgcgctgt caacggtctt cgaagtaccg
     6421 cttccgtgct ggaccgtcgc acgcgtgtag atcaccgacg tagcgggatc gatgaggttt
     6481 gcgcgcgagg tgaccgcgat gaagttcgcc tccatgtcgg cgctgctcgc cgacggcatc
     6541 gctttcaacg tgaacccgcc ctggccattc gtgtgacacg cggcgcccgt gcagcgctcg
     6601 agcatcggct ggatcgtcga ctggtacacc ttgtagtcga acgggctcgg gccggggggc
     6661 ggcgtggttc caccgccgtc agcggcgtag cgtgccgagg ccgtctcgac gaacgtgcgc
     6721 aaggtcgcca gctccggcgc cgtcggctgg aacgtgaacg gatgcgtcgt cgagccgccg
     6781 gagatcaccg cgatgaaacg gctattcgtc ggcgtgacga ggtcgacctt ggcgacgacg
     6841 ctgttgaacg tcttcgcgaa atcgcagtcg ccgggtttcg ccgcggccca caccgtgaag
     6901 gtgccgttgc cggtcggtgg agcgtggcag ccggccgccg cacaactctt gccgatgacc
     6961 ggcatcacct cgctgccaaa cgtgtcgacg tcgaatggat acccgggcgt tttctcgctc
     7021 ttggtgtcga gctggcactc gggtgcggtg gcgccgggat cgtcgccgtt gatgtcgccg
     7081 ttacacgcgg cggcgtgggc cgcgagccac acgacgaaca acgatcgcaa atgatgtgtc
     7141 atggaaatgg tgctccctct tgcgcagtgc ggacgctcac cacgtagcaa cgagcattcc
     7201 cggcgagtga ctcagcaaga tcatttgcag tcgtcgcagc gagcgcgatt cggtgtctgc
     7261 cggcaccaac aagacgcgga gacgttttcc ccacgcgatg atcggaggtg actactgccc
     7321 cagttacgtc gctgtatgcc gagagctacg gtcgaaacat gagaacgcgt cgactgaatc
     7381 gcgaccgtgt ggagatagcg aggttccgct tgcgcggcat atcgggcccc gatgcaccat
     7441 ttgtggtcga cagcgagacc gccgcggtca cgatcggcac ggcgcaagcc gagctcgtcc
     7501 agctgaccga tccgtgtgtg tcacgacacc attgtgtgat ccgcgtcggc gacaacggcc
     7561 tcatctgcga ggacctcgga agcacgaacg gcacgttcgt cgccggtcgc cgcatcacga
     7621 gtgccgtgat cgatccgggc gacacgatca tcgtcgggga gaccgtgatc gcaatcgacc
     7681 cactcgacgg cacgatcagc gaaccgctgt cgcacaatga tcacttcggt ccggtgctcg
     7741 gccgcagcgc ggcgatgcgc cacctgttcg cgatcctcga gcgcgtcgcg ccgtcggatg
     7801 caaccgtgct gatcgagggc gagaccggca cgggcaagag catgctcgcg gaagcgatcc
     7861 acgctgcgag cccgcgcgcg aagatgccgt ttgtcgtcgt cgactgtgca tcgctgccgc
     7921 accacctgct cgagtcggag ctattcggtc acgagcgcgg cgcgttcact ggcgccgcca
     7981 acgatcgcat cggcatgttc gaatccgcga acggcggcac cgtgctgctc gacgagatcg
     8041 gcgagctgcc actcgatctc caacccaagc tactgcgcgc gctcgagcgc cgggcgatcc
     8101 gccgcatcgg cgcaacccag gtgacgccga tcaacatccg catcatcgcg gcgacaaacc
     8161 gcgacctgcg ccgcgccgtc aacgcacacc gattccgctc tgatctgtgg taccggctca
     8221 acaccgtccg cctcgtgcta ccgccactgc gcgagcgccg cgaggacatc ccgagcctcg
     8281 tcgcgtcgct ctaccgcgag ctgctcgccg atccgaaggc gatgccgccg gcggaggtcg
     8341 tgcgaccgat gatgcgcgcc gattggccgg gcaacgtgcg cgagctgcgc tccgcggtcg
     8401 aacgctcgct gctcggtgcg acgccgtccg ctaccggttc gatgcccgac atcgaggagg
     8461 gcctgccgtt ccgccacgcg aagaaccgcg cgtcatcgga atgggagcgc cgctatctga
     8521 gcgagctgat gctcgccagt ggcggcaaca tctcggcggc cgcacgcgcg ggccagatga
     8581 accgcagcca tctcagcgag ctgctccgcc gccatggcct cgtcgggatt gccgacgaca
     8641 tcgcgttcga gcccgatgcc gaggagtcct cgccaggacg ctagtcgcgc tcttgctcgc
     8701 gcgccgccct gtcctcgcct tcgtcgcact gcttgcccat cgagtcgagg cattcgagcg
     8761 cgacgcgatc gagcgtctcg ggcatgtcga gttgcgacag cgcgggcagt ggctggagct
     8821 tcttttgctt gagctcgtcg ctgagcgccg gcgcgtcctt cgtcgcgacg gtcttcagcg
     8881 tcgcctgcac gtccccgagc tccttcttga gaacgtccag gcgctcgagc tggtacggcg
     8941 tcggcttgcc ttcccagccg ttcatcgcgc cgtacagcgt gtcgaggtgc tcgcggatgc
     9001 gttcctcgcc ggtgatcgcg ccgccttccg tggtggcgac gacgagcttg cgcgcctcct
     9061 cgagcttgtc atcgagcttg cgcagcttgc ccgcgagcgc gtcactggcc ggcagtgcct
     9121 tcgcacgctc ggcgaccgcg ccacgcagcg cctcgatctg atcgacgagc ttgctcatgt
     9181 cgttgaacag ctcgtgcgcc ttcatcaccg ccacgaactg cgccttcttg cttgccgcgt
     9241 cgtacggtgc acggcgatcg agaccgatcg cgagcttggt ctcgagcacg tccgctccgc
     9301 gcgtgagccg caccgtgtac gtgcccggca cgacgcgcgg gccttgcgac gcgctgaacg
     9361 cgacctgggc ggcacgcgga actcgcgggc ctttcacccg catcgtccac gtcacgcgat
     9421 tgatgccgcg ccgcttggtc ggcgtcacgg tgtcgatcac cttgcccttg tcgtcgagga
     9481 tctcgagctt gatcgggccg tagatgtggc gcgagcgcag gtagtacgtg atcaccgcgc
     9541 cgctcggcgg gttcgcgccg acgaacgtcg catcaccttc gacccaaccg ccactcgact
     9601 gcatgcgctg ctggatcggg cgctgcggca agaacacggt ctgcttctcg agcgtcggcg
     9661 cagtgagcgc gcgcagcggt gtgatgtcgt cgatgatcca gatgccgcgg ccgtgcgtcg
     9721 cgatcacgag atcgttgtcg cgcggttgca cggcgaggtc gcgcaccgcg acggcgggga
     9781 agtcgccgcc cttgaactct gcccacgtgc caccgccgtc gagggagatg tagagaccga
     9841 gctcggtgcc gaggaacagg aggttcttcg cgaccgcgtc ttccttgatc acgtgcgcgt
     9901 agccgcgaac cttttgctcc ggtccgacga tgcgcttcca cgtcttgccg aagtcggtcg
     9961 tcttgtagac ccacggcgtc atgtcgccga acgtgtggcg atcgaacgtc gcgaacgcgg
    10021 tgcccgcgtc gaaccggctc gcttcgaccc agctcaccca cgaattcggc ggcaggttcg
    10081 gcacgttgcc gacgacgttc gtccatgtct tcgtgccatc gcgcgagatc tggacgttgc
    10141 cgtcatcggt accgacccac acgacctgcg cgttcttcgg cgactcgctg atcgagtaga
    10201 tcgtcgtgtg catctcggcc gacgagttgt cgacggtgac gccacccgat tgctcctgct
    10261 tctgcttctc cttgtcgttc gtcgtcagat ccggcgacac gcgctgccac gtgttgccgc
    10321 gatccttcga gcggaacaaa aactgcgagc cgagatagat cgtgcccttc tgcgtcgggc
    10381 tgactgccat cggcgtgttc cagttgaacc ggagcttctc cttgtatttc gccttcggtt
    10441 ggatgtcgcg cgacaccatc gtcttgcgat cgatgcgatt gatgtaaccg ccctgcgact
    10501 cgcagtagac cgcgttcgga tccgtcgggt cgggaatcgt ccagaagccg tcaccgccgc
    10561 acaggttctc ccagcgctgg ttcgtgatgc cgccgccgta cgcgctgtcg ccgacccacg
    10621 agctgttgtc ctgcagcccg ccgtacacct ggtacggatc cttgttgtcg atgctcacgt
    10681 gatagaactg cgagactggc aggctgttgt tcttgtgcca gcgcgtgccg ccgtcgaacg
    10741 acagccagag gccgccgtcg tcgccaccga tcacgtgctt gggattctgc ggatcgaccc
    10801 agacgtcgtg ccagtcgccg tgcgacccgc cgcccgaata cgagaagctc ttgccaccgt
    10861 cggtcgagac gacgaggttc aggtccggct tgaacaggcg gttcgggttc gtcggatcga
    10921 cgacgagacg cgcgaagtag aacgggcgcc acaccatcat ctggctcttg tcgcgctcgt
    10981 cccacgtctt gccaccatcg tcggagcgat acagcgccga cttcttcgac tcgatcagcg
    11041 cgtagacgat cttggaatcc gacggggcca tgacgacctc gacgcggccc cacggcgttg
    11101 ccggcaggcc cttcgctttc gctgcattga tcggcgtcca cgtcttgccg ccgtcgctgg
    11161 agcggaacat gcccgatgcg ctcgctgcat ccgggccgtc gccgccggag cggaacgtcc
    11221 agcctttgcg acggaagtcc cacatgcccg cgatcagcga gtcgggattc ttgctgtcca
    11281 tcgtcacgct cgagcagccc gtcgacgcgt tcgcgccgcg gagcacgagc gaccacttct
    11341 tgccaccgtc ggtcgtcttg tagagaccgc gatcgttgct gtcgctccac agcttgcccg
    11401 gcacgcacgc gtatacgacc tggctgttct tcggattcac gaggatgcgc gtgatgcgct
    11461 cggagtccgg gagtcccagg tttgtccagg tctcgccttc gtcggtcgac ttgtagatgc
    11521 catcgccgat cgagaccgag ttacgcgtcc acgactcgcc ggtgccgacc cagatcgtgc
    11581 gcgggttcga cggatcgatc gtgatcgcgc cgatcgactg cacgcgctgc ttgtcgaaca
    11641 ccggccgaaa cgtcgtgccg ccgtcgatcg acttccacac gccacccgat gcggcgccga
    11701 cgaacaggat cgtcttgcca ccctcgttgc gcgccgcgac tgccgcgata cggccgctca
    11761 tcgcagccga gccgatgttg cgcgcaccaa cgcccgagat ggtgccggca tcgaccacgg
    11821 ccgggccggt cttcagcggc ggtgcggccg tcggcgccac cttgtcgggc ggaccggcaa
    11881 ccacgacgcg cgtcacggcc ttcgtcggca gcttgaagat gtcgtcctcg accgggacgt
    11941 tgatctcggc gcgctcgacc tgaacgcgga acccacgcga cccgcccggc gagccgctct
    12001 cgatcgagaa cggaatccac acgccgccga cctgctgata gccgccgaag tcggtctcac
    12061 cgatttcctc ggtgccgcga accttgtgca cggtcgtgat gcgaatctcg agaccggact
    12121 cgggatcgag aaacacgtac tggatgtcgc cgtccttgcg ggtcacgcgc agcttcgccg
    12181 cgagcgtgcc gtcgacgtcc tcggtgccga gatactcgac cttgtgtccc ttctgccgcc
    12241 agtcgatcag cgggccgtcg aggtcggcgt cctgcgcgaa cgagcgggcc tggtcctcag
    12301 atgctttctc ggcgtcgcgc cggccaccga agggccgcac gctccagccc tctttgccgt
    12361 cataggcgtt cacctgcgtg aggccctgca acgtgatctc gttgcggacc ttgccgctgc
    12421 gagtcgtgat cgatccccac gcggcttcga gcgaaaagtc gccgccgccc atcgtcgctt
    12481 tgccggtgag gcgaagcgtg ttgatcgctc gcagcttctc ggcgccaccc cgtgcagcga
    12541 ggttgcgcat gagaatgtcg tcgagcgcgt cggcgtgcgc gcggtgtggt gtcgaaatca
    12601 gaagcagagc aaacgcaatg agagctcggg tccgcatcgc ggcggagtat ccgcgaaaac
    12661 gcgcggtgtc gcagagtgtc gcgggcgttc tcgacgcccc cgcaacttct gccgaggtca
    12721 cttcatgtac ttctcgacgt tcttcttgtg ctcgtcgaac gtcttggcga acgcgtgctc
    12781 gcggtcgttc ttcgcgacga agtagagata atccgagcca tccggtgaca cgaccgcttc
    12841 gatcgagctg cggcccgggc tcgcgattgg ccccggcggc aggccttcga gctgatacgt
    12901 gttgtacggg ttgtccttgt cgtcgagctg cgcgcggtgc aggcgatcgc agcccggcgg
    12961 caggttctgt ttcgcgcacg gctcgttcca cgcgacgcac gcggtgctct tcttctccgg
    13021 caccaggcag ccgtagcgaa tcgtcggatc ggtctcgagc cgcttcggct tgaagtccgg
    13081 cttcgtcata cgattgatga agacctgcgc gatgcgcggc cgttcggtcg gctcgaccgc
    13141 ttccttctcg acgatcgacg ccatcgtcag gatgtcgcga tcggtccacc gcatcttgtc
    13201 tttgatccgc gccgcgtcct tcggatgctt cgctagcacg tcctgccaca cttcctggta
    13261 gcgcgtgatc atgcgctcca gcacgacgcg cggtttctcg ccgacgcgaa actcgtaggt
    13321 gtcgggaaac aggtaaccct cgacggtgtc gccgacgatc gcgtggcccg cgagaaaatc
    13381 tttgtcgcgc gcgagctgct cgagctcctt cgcgctcgtg atcttcggac acggcgcctt
    13441 catgccgacc gggcacgcct tctcgatcag ctcgaagtac tcgagcatgt tcttgccctc
    13501 gggcagcttc acgcgcgtcg tgatctcttt gacgcccgtg atgatgatct tgagcacctg
    13561 cgccggcgtc aggttgtcct tgatcatgta cttgccgggc ttgatgttcg tcgtgtcgcc
    13621 ttcccacatc gcgtagaggc gaaaccaggt tggccgctcg atgatgtgct gcgacgcgag
    13681 cagcgaagcg accgccggga agctcatgcc gctcttgatc tcgacttcga tctccttgcc
    13741 cgagccgctg tgcgcttcat cgggataggc gaacgcgcgg tgaacgaacc aacccgcgat
    13801 gccgaccacc aagatcaccg agccgatgac cacggcgagg gcgacgcgga acgagcgctt
    13861 cgacatcggg ggaatgacct tactagcgag ggcgctggcc gtcgagccac gcctggagaa
    13921 tgagcgcggc ggcttgctga tcgatcactt gcttgcgctt ttggcgtgac aagtcggcat
    13981 cgagcagaac gcgctcggcc tccgccgtgg tgaaacgctc atcctgttcg tgcaatttca
    14041 ccgcgtcgcc gagttttgcc tgcaaggcat tcgcaaattc ttggacgcgt tttgcgcggt
    14101 ggccgacctt gccgctgagc tcgaacggca tgccgaccac gatttgccgg atgtcgtgtt
    14161 cggcgacgag tgtttggatc gccgacacat cagcgtcggt tcccttacgc tcgagtacgc
    14221 gcagcggatg cgcggcaatg cccacctcgt cggtgatagc tacaccgatc gtcttgcttc
    14281 cgacgtcgag gccgagcacg cgcatcgtcg gaagtgtccg gaaaaataaa ggccctgcaa
    14341 gggggattgc agggcctggg cgcagatcaa agggctactt gttgcctcag gtggtggggg
    14401 agaccagcgg gatgaggggg gagggaacca ctagtcatcc accgagtcga aactttggac
    14461 acctttgtcg cgcgtgggat tcagtttggt tttcagaacc aggccgtcgc ttcggctatt
    14521 ccttggaacg ttccaagcgc cgtctggttg catggtttct tgagcatgtt tgatgccacc
    14581 gaaaaacgag atcgcgccga cgaggaatcg aggccttagc taggcccccg tgcccgatcg
    14641 gcaacaagtc ctgtcacccc tggttctcgg gctggccagt ctggtggcca ccctgacgaa
    14701 tttgcttcgt ggttgatcgc gattcaaaag cggtttcaac gtccgaccac gcgatacgtt
    14761 cgcgttctca tgtcggacga acctgctgcc ccacccaagc tccaagtaca gatcgacgat
    14821 gatatttcgc agggcgtcta ctcgaaccta gtgttgctca atcacacgga gaacgagttc
    14881 gtcctcgatt tcgcgttcat ccaaccgagc aacggtcgcg caaaagtgcg cacgcgcgtg
    14941 atctcgtcgc cgcgccacac gaaacgactg ttgcttgcac tgcaaaagaa tctcgaacgc
    15001 tacgaagagc gcttcggtcg tatcgagctc ggtgcagacg aagagccgat cgttcattga
    15061 tgcggcgcgc gctcccgacc gcacgctgcg gcgctcggtc tcactcgctg cggacatggg
    15121 gttgcgtagc gagcgcagtg tgatggcgcg caacttcgag tgagacgatc gcaaggcggc
    15181 aaacctcggc ccgtgcgccc atgcgcgcat cgcgataccg gaagtcgcga acgcgaagtg
    15241 atcagtcgac tgccggatcc aggatctcga tcgttccggc atcgagatcg aggatcgtgt
    15301 cggcgccgaa cggcaccgct tcgttgcgat cgccgtggcc gatcggtgca ccgaccgccg
    15361 cggccgttcc cgctgcgcgc aatcgctcga gcaccgtctg cagcgccgca tcttcgggat
    15421 ccggcacgcc ggtcggcgga ttcgcatcca cgcagcgcgt gagatcaccg acgacgactg
    15481 ccgcgagctg cgcgagctcg ccggtaagcg cgagctgcgt gagatagcga tcgagctcgt
    15541 acggccgttc gccgacctct tcgatcaccg cgatcgcgcc acgcaccgag acctcccacg
    15601 gcgtgccgac gagcagcgag tacatcgtga ggttgccggc gacgagcgga ccgcgatagc
    15661 gccccttgcc gtgactcgcg agctgccacg gccgcacgcc cggcgcacgc ggcacgctga
    15721 tcagatcgat catgtgcgtg atgtgctcgg caggcagccg atcgagctgc acgaccatcg
    15781 gcccgtgcac gccgcgcacg ccagcggcgt atgcccacgc caggagcgcg gtcgcgtcgg
    15841 agaaaccgat gatcggtttc ggatcgcgac gtagcgcctc gacatcgagc cgcggcagga
    15901 tgcgcatgag gccgtagccg ccgcgcgcga gcaagatcgc gcggacgtca cgatccgcga
    15961 gcatcgcgtt gagctcggca gcgcgcacgt cgtcgctggc cgagaggtaa ctcggcacgc
    16021 cgggcggatg cggcgcgagc acgctgtcgg cgacgcgcag cttgaatgca tcgccgagct
    16081 gggcgagccc gcgctcgagc acagcgcgct tcaccggccc gcctggcgcg acgatgccga
    16141 gcgtctgccc gcgttcgatc ttcggtgggc gcgcggcgat tgccattgcg ctcagggtaa
    16201 cgcgctaacg gcgctttttc ttcttcttgc ccttctcgtt cttgtcgccg ccgttgccgg
    16261 tggcaccgga atcctcgtcg gaggccgcgg gcatcgagcc gctgctcgcc gcaggatcca
    16321 aaccgagcgc ggcgagatcg acggcatcca tcatcatcgt gcgcttgccg tcatcgacga
    16381 tcggattctg cgcggcgatc tcggccgaga tccgcggcag cgacggatcg gtgacttcct
    16441 tctgcgcggc cagctcgccc gacgcgccgg cgctgtcgtc gaccgcatcc atcactgcgg
    16501 tgttgcggcc atcgtccatc agctcgctcg gagtggcgtc gccggtcgag cgccgcgatc
    16561 gcacggtcac cttgccctca gcatccggct tcggcagcgg cttctcgtcg accgtcacgc
    16621 gctgcttcca ccacgagcgc tgcgcgtcgg cgtcgagctt cggcttgacg accatgccgg
    16681 gcggcttgtc cgagccagtc acttgctcgc gcacttcggc aaccgttgct tcgaacagca
    16741 cgccatcatc ggtcgacatg ccgatcgtgg tcccgaccgg catcggcgtt ggcatctcga
    16801 gatagccagc ggtcgcccga acctgcgtca gcttgatgcg cttgccgaga gcgagccccc
    16861 gataactgag atccacgaag ctctcgctca tcgctgccat gttatcgcat gctccgcgcc
    16921 gtgggtttga tcgaccgcct aaggcaactt cggacgaaca cccccgccga tgacatcgac
    16981 gggcggacga aggcgctcgt cgtccacggc aacgagttcg ggttcgactc gttcggcatg
    17041 tccaaagcga gcctgcgccg cgcgatcatc gtggcgcgct ggctgtatcg ccattacttc
    17101 cgcgccgagg cgttcggcat cgagcacttg ccgccgtccg gccgcatgat cgtggtctcg
    17161 aatcactcgg gccagctccc gttcgatggc ctcgtgatcg cgagcgcgtg cttcctcgag
    17221 ccgccgcagc cgcgcatggt ccgcgcgatg gtcgagttct tcgtgccgac cgtgccgttt
    17281 gcgtcctatc tgttttcgcg ctggggacag atcacgggca cgcccgagaa ctgccgccgg
    17341 ctgctctccg ccgaagaagc ggtgctcgtg ttccccgaag gcgcgcgcgg catcagcaag
    17401 ccattttcga agcgctacca gctcgccgag ttcggcaaag gcttcatgcg actcgcgctc
    17461 gaaacgggct cgccgatcgt gcccgtcgcg gtgatcggcg ccgaggaaca agcgcccgcg
    17521 atcaacgtca agcccctcgc acgcctgctc aagacgccgg cgtttccggt cgtgccgtat
    17581 ccgccgttca tcgcgccggt accgctgccg gtgaagtacc gcctgcactt cggcgagccg
    17641 atgcacttca cgggcgatgc cgacgacgat gacgagatcc tcgacgagaa agtgaagcag
    17701 gtgcgcaacc ggatccagtc gatgatccac gtcggcctgc gcgagcgtga acatgtcttc
    17761 tggtgaacag caggcgatca ccgatcgctc gatcccgcgg ccgaagaagg tcgtgatcac
    17821 cggcatcagc ggccggttcg ggcacatcgt cgcgcgccgt ctccatcacg atctcggctg
    17881 gtcgatcgtc ggtctcgatc gccggccgat gtcggggcgc ccgaaagaca tcgagcacca
    17941 tcaggtcgat ctgcgcagca agaaggcgcg cgacatgttc cgccagggcg acgtcgatgc
    18001 gctcgtccac ctcggcgtca tgcacgatcc gcgtgcacgt cccgccgagc tgtactcgtg
    18061 gaacatcacc ggcacgacga agctgctcga gtactgctcc gcgtatcgcg tgccgaaggt
    18121 cgtgctgctc tcgagtgcga acgtctacgg accgcgtccc gacaatccgc agttcctcac
    18181 ggaagaagca cctctgcttg ccgcgcagcg gtttccgcag atgcgcgatc tcgtcgagat
    18241 cgatcacctc gtgtcgacgt ttctgtggcg cgcgcgtgag accgagaccg tgatcttgcg
    18301 gcccgtccac atcgtcggcc cgatgcacaa cgctccgtcg aattacttac gcatcgaccg
    18361 tccacccacg ctgctcggct tcgatccgat ggtccagctc gtacacgccg aggacgtggc
    18421 agaagcgatc gccatggcgc tgcagcccaa tcggcgcggc atctacaaca tcaccggtcc
    18481 gggggaagta ccgctgtctg cggtgctcaa agagctcggc cgggagccgc ggtcaatccc
    18541 gcatccgatg gcaagaccga tcctcggcct ggcgttccgg ctggggttct cgagcttccc
    18601 ggtcgccgag ctagacttca tccggtacgt ctgcatggtc gacggtcgcc gcgctgcggc
    18661 agagctgggg ttccggccgc gattcggcct ccgcgagacc atccacgcgg tcgacgatcc
    18721 ggtgtaacgg aacccccacc ctgggggcac caagtgccta ctcgtgccgt atcacctagc
    18781 cacaggtggt gtagccgggg gttcacacca tcaatccgat acgttgctcc cgatcccgcg
    18841 atgacaatcc ctgccaacgg caaccacgac agcgagtgtc gagtccctcc cccgtcggtg
    18901 gcgatttttc gggcattttt gtcaggcttc cggcggcaat tcccaacaga ccatcttggc
    18961 gtgtcggaac gttaatgcga ttgcgggcag ttcgatccct ttgtccgaat ggcacgagga
    19021 ctgcttcgtc ccccctcacc gcacgcccca ttcccccaag gaacgatccc ccatgaagaa
    19081 cctctcgctc ggtctaatgg tcgccttcac gaccacagtc ctgtccggct gcgccctcta
    19141 cttcggcgag gacaaccaca acgacgatac gtggacctac tgtggctccg acggctacta
    19201 cacctgcgac ggtgataact gccagtgggt gggctcgtcg tgtcccgacc aaggctcggg
    19261 cagtgcccag ccaggtggct tcgactgccg cgataacacg gactgcgccg ccggttgcta
    19321 ctgcgcaaac ggtacctgcg aagaggcagg cttctgcacg caggacagcg attgcggcac
    19381 ggggtacgtc tgcaacgagg atcggtcgtc gtgcgaaccg ggtggccaga cctgcggtgc
    19441 cgatgcggat tgcgatgcgg gaagcgtgtg cacggaaggc agctgcacca cgacgtgcgt
    19501 ctgcgccaac gatgcggaag cagctgcgca gggatacgcg tactgcgacg agcttcgcat
    19561 cacctgcatg agcggcacgg acccggctgg cacctgcggt ggcgagccga cctgcaacac
    19621 gctgccgcct tcgtgcgccc ccggcgatgt cccgctcctc ggcgcggacg gctgctggaa
    19681 cggccagtgc gaagcggcga gcgactgtgc ggtggccccg agctgctcgc acattcatga
    19741 cgaggcgacc tgcctcgcgc aggcgggctg tgcggagacg gtgaacggca tcaactgcaa
    19801 gaagcccgac ggcacggcct gcatggctgg ctcggcgaac tgcacgtgcg acagctacgt
    19861 cttcgcgagc tgccgctcgg cgacgccctg aagtagcgag acgttgacga cagcgtggcg
    19921 gcccctccgg gccgcttcgc attttcggcg caaagcgtat gctctgccca tgggcttcga
    19981 gttcgccgaa acgatgtccg gcacgatcga atgggatgcc gagccgggca agaagcaccc
    20041 gttcaagttc gagatccgcg cccatgctgc gtcgacgcgg gagcacttcg cgaacggtcg
    20101 cgccgagatt cgcggcgtga tttcggcgcc gccgaaggca gcctccgccg atgctgaggg
    20161 cacgatcgtg atccgccccg tcggtcagaa gatcatccgc tacgagcttt cgttcatcgc
    20221 cgatgacggc aagcgctacg agatggtcgg gcaaaaagac atccgctgga gccggccact
    20281 cgagaccttg acgaccctgc ccgccgaaat cctggatgag aaccaccgtc gggtggctac
    20341 ctgcctgact aggttcgact accggaacga tttgtggtcg ttcctgaggt cctttcgtcc
    20401 ggcttgacgc ggccagaagt ggtacacacc ccttctggtt atgggactga ttcagaagat
    20461 tttcggcagc aagaatcagc gcgagctcaa caagctccag cccaacgtca atcgcatcaa
    20521 tcagctcggc gactcgtaca aggcaaagag cgacgccgag ctccgcgcgg tgaccggcga
    20581 gctcaagcaa aagctcgata atggcgcgtc gttagacgat gtcctgtgcg atgcgttcgc
    20641 cgcggttcgc gaagcctcga tccgcacgac cggcatgcgt cactacgacg ttcagctggt
    20701 cggcggcatg gtcctccacc agggacgcat cgccgagatg cgaaccggcg aaggcaagac
    20761 gctcgtcgcg acactcccga cctacctgaa cgcgctcgaa ggcaagggcg tccatgtcgt
    20821 caccgtcaac gactacctcg ccaagcgcga cgccgaatgg atgggtcgca tccacggctt
    20881 cctcggcctc accacgggcg tgatcgtcca tggcctcgac gacttcgagc gccagcgcaa
    20941 ctacaacatg gacatcacgt acggccagaa caacgagttc ggcttcgact acctgcgcga
    21001 caacatgaag acgtcgcccg atcgcatggt gcagcgcggt ctgcactacg cggtggtcga
    21061 cgaggtcgac tcgattctga tcgacgaagc gcgtacgccg ctcatcattt ccggctccgc
    21121 cgaagcatcc ggcgacctct acgtgaaggt cgaccgcctg atcccgcgcc tcaagcgcga
    21181 ggtcgactac acggtcgacg agaaggcgca ctccgcgatg ctcaccgacg acggcgtcga
    21241 gaagatggag aagctgctcg gcgtcgacaa cctgttcgaa gcgacgaaca tccagcttgt
    21301 ccaccacgtc cagcaggcac tgcgcgcaca cacgctgtac aagcgcaacg tcaactacct
    21361 cgtcgaagac agcaaggtcg tcatcgtcga cgagcacacc ggccgtaaga tgccgggccg
    21421 ccgctggagc gatggtctgc accaggcgat cgaagcgaag gaaggcgtcg aggtcgaaga
    21481 agagaaccag acgctcgcga ccgtcacgtt ccagaactac ttccgcatgt acaagaagct
    21541 ggccggcatg accggtacgg ccgacaccga agcgcaagag ttccaccaga tctacaagct
    21601 cgcggtcacc gtcatcccga cgaacaagcc gatcgttcgc aaggacttcc ccgacctcgt
    21661 ctacaagaac gaggcaggca agttccgcgc ggtcgccgag gacatcgagg actgttacaa
    21721 gcgcggccag ccggtgctcg tcggtacggt ctcggtcgag aagtccgagg tcgtcgcgaa
    21781 catcctcaag gcgaaggggc tgccgttcaa cgttctgaac gcgaagcagc accaacgcga
    21841 agcctcgatc gtcgcgcaag cgggtcgcaa gagcgcgatc acgatcgcga cgaacatggc
    21901 cggtcgcggt accgacatct tgctcggcgg caacttcgaa gcgatggcga aggacgcgct
    21961 gcaagaagag aaggacaagc tcgcgcagga agcgctcgcc gcaaagccag gcgaaggtga
    22021 cgtgcccgag ggtgacagcc cgtatcgcgg ctcgagccag cctgatttcg acgaagagaa
    22081 gcggctcaag gagctgaccg aggaattcaa gaagcagtgc gaagcggagc gcgaagaagt
    22141 gctcgccgcg ggcgggctca agatcgtcgg caccgagcgc cacgagtcgc gccgcatcga
    22201 taaccagctg cgcggtcgcg ccggtcgcca gggcgatccg ggctcgtcgc ggttctatct
    22261 gtctctgcag gacgacctgc ttcgcatctt cggtctcgat cgcatgacgg gcctgatgga
    22321 gcggctgggt ctcgaagagg atgtgccgat cgagtcgccg atggtgacgc ggtcgatcga
    22381 gaacgcgcag aagaaggtcg aaggccgcaa cttcgatcag cgcaaaaacg tcctcgagta
    22441 cgacgacgtg atgaaccagc agcgcaagac gatctactcg ttgcgccgcc agatcctcga
    22501 gggtcgctac tacccggagc cgaccgagga ccaacagaaa gaggccttcg tgcccgagcc
    22561 ggtcaccgag agtggtgact ggacgcaaga gacgatccgc cccgacgttc acctcgtgct
    22621 cggcaagatg atcaccgagc taaaggcgaa ggtggccgaa cgcgacgaga agctcgcgcg
    22681 cggcgaaccg gtccaggaca cgcgccccgg ctggcgcgtg ctgcgcgccg agatctggcg
    22741 ccagttcggt acgtggctcg atctcgagaa gcgctactcg aaggacgacg acctgctcga
    22801 gtgggcggcg acggaagtcg cggcatcgca gatccagcag cgcgagcgtc tctacgatct
    22861 cgcgcacacc gagatggcga acgtgatcga gcacgtcctc gatccggaga tgagcgagga
    22921 cgagtgggac tggaaccagc tcgacgaagc gctcaccgag gcgttcgaca ttccgtacga
    22981 gctcagccat ggcacgcccg acgaagcggc ggcgcaggcg tggccggaga tcgagacgaa
    23041 gcttgcggag cgcgagaaag agctgacgcg cccgtggctc atgtacttcc tccgccactt
    23101 ctatctcgag gagatcgatc agcaatggat cgagcacctc aagacgatgg atgcgctgcg
    23161 cgaaggcatc ggcctgcagg gctacggcca gaaggatccg aagaaggaat acaagaaggc
    23221 cggcttcacg ctgttcaccg agatgatgga acgcatccag gagaacgtga tcaagaaggt
    23281 cttccacgtg cagatcaagc gcgaggaaga atcgattccg gcactgcagg ccgagaagca
    23341 acgccagatg gtcgagcgcg gcgttgccga caaggcggac gaggaagtcg acgccgaggt
    23401 ggccgcgaag gcggcgcggc gctccggcaa ggcccggacg tccggcgatg gcccgaccac
    23461 ggcaccgatc aagcgcgagc gcccgaaggt cggccgcaac gatccctgtc cgtgcggctc
    23521 gggcaagaag tacaagaagt gccacggtaa agacgaagaa gcgtccgccg agggataact
    23581 ccgcctccgc cgcactttcc atgcgacgtc gtcccgctcg tgggttagcg ggacatgaac
    23641 aacaagcttt ggatcctgtt cctgctcggc gcgtgtggtg gcggtgatgg cgccggtggc
    23701 ctggcggtcg acgtcgagaa cccgtcggcg ttctgccgcg cagtcgccga gaccggctgc
    23761 agcacgatgt acgcctgcct caccgatgcc gagcgcatgg cgaagcactt gccgatgacc
    23821 gaggccgagt gcgagcgcca gctcgaatcc gggtgcgaag atgcggtcga cagctgcaac
    23881 gacaacacgc acggctacgc gagcgacgcc gcaggcgcgt gtatcaacga gatgcaagcg
    23941 gcgacctgca acgacgcggg tgagccgtgg ctcgatgcgc cgtcgtgcag caacatctgc
    24001 gcggtcacga aaggctcgtt cgaggtgcag tgggcgttct cgccgtcgta ccactcgtgc
    24061 agcgagctcg gcgtcacgac cgtcagcgtg tactcgatcg cggcgggcgg ccgaacgtac
    24121 gtcgacacgt acgactgcta caccggatcc ggcatcacgg atgttctgcc ggtcggtacg
    24181 tactcggtgc acctcgagct gttcaacgcg agtaacca
//
LOCUS       DASUFX010000738         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_396362,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000738 DASUFX010000000
VERSION     DASUFX010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396362"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFV99_29840"
     CDS             <1..291
                     /locus_tag="VFV99_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX5063631.1"
                     /translation="SGQIALDPATGAMIEGDVEAQTRRVIANLAAVLASAGASFADVV
                     KTTIYLKSMGDFTTVNGVYGEHFVHEPPARATVEAAALPKGALVEIDAIVVF"
     gene            301..2253
                     /gene="acs"
                     /locus_tag="VFV99_29845"
     CDS             301..2253
                     /gene="acs"
                     /locus_tag="VFV99_29845"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5063632.1"
                     /translation="MSSSIESVLTENRSFAPSKEFAAASRVGSMADYEKLYRRADEDP
                     EGFWGEVAGELAWAKPWQRVLDWKLPDAKWFVGGRLNASASCLDRHVTTWRKNKAALI
                     WEGEPGDSRVLTYGQLHREVCKAANALIELGVSAGDFVAIYLPMIPEAAIAMLACARI
                     GAPHTVVFGGFSSEALADRIKDCKAKVVITSDGGWRRGAIVPLKENVDRAIEGTTVTQ
                     VLVVKRCANQVAMKTGRDVWWHDLVDSAPPRHEVDSFDSEHPLFSLYTSGTTGKPKGI
                     LHTTGGYLTGAYYTTKMVFDLRDDDIFWCTADIGWVTGHSYVVYGPLANGATVLMYEG
                     APNQPGPDRFWQIIAKYGVTVFYTAPTAIRAFMRWGDAWPAQHDLTSLRLLGSVGEPI
                     NPEAWMWYRKQIGGDRCPIVDTWWQTETGAIMISPLPGATPTKPGSATRPLPGIAADV
                     VLKDGTPCKANEGGFLVIKKPWPSMLRTIFGDHDRFVKTYFGEVQGCYFAGDGARKDA
                     DGYFWVMGRVDDVINVAGHRLSTMEVESALVSHPKVAEAAVVGRPDDLKGQAIVAFVT
                     PRQNFVADDALAKELREHVVGQIGALARPEEIRFAEALPKTRSGKIMRRLLREVASGS
                     KSRGDTTTLEDQGVLERLAQTFAQDD"
     gene            complement(2286..4052)
                     /locus_tag="VFV99_29850"
     CDS             complement(2286..4052)
                     /locus_tag="VFV99_29850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX5063633.1"
                     /translation="MKRALVLIVAMLLASPVAMAQPSKTRAAKVLAKPAKAVVAKAAD
                     KDKKKKAATTSRARVAFGKGGELRPAREVIGRREEPLTLEEDIAKQIEKLLRGPLRNG
                     ITGLYVADARSGEPLFAVNSDDPLNPASNVKMVSTATALELLGPTFRYTTRVLGPEPD
                     AAGVVHGNLYLLGTWDPTLAAGDIDDLAQKLTARGVKQLDGNVLSGSDPTRDGIFRAV
                     VPIAITAGEPGQPPTATTPAGFDLVTVDVTAKTEKRARKRPKLTYKQELTTDAAGHKR
                     IKLTIGGTIGKGGETMYALYTKERTAVAAHQLRAALRAYQVVVNGDISTIELGDFIGD
                     QVGSKGLPVELARHESQTLGDIVTRVNKWSINWLADRVVMTAAALSRRTTPSMDVAID
                     AMYAWMQRHANLSKDSVVIDTGSGLSYRTRITPHELVAIVRSAGGFAQDGDQAVAKAW
                     LGSLSIAGTDGTLRHRFRVPEIQGHIHAKTGTLSTAIALTGVLDLDPTRPLAFSIITN
                     SLRPLRKGYVRKAHEQLVALLAKYVVATAKPTSLPAGTPITFGKPTTSLPAINETAVT
                     VPDEIAEPQFDPELDVETSTSK"
     gene            complement(4049..4591)
                     /locus_tag="VFV99_29855"
     CDS             complement(4049..4591)
                     /locus_tag="VFV99_29855"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5063634.1"
                     /translation="MKGKSTGSRVKRAVLTPEDQSLFLEAMGGVTPLESRDRVNVPPP
                     PPSPVRVVELPPEVKLTVDGDAARYAARSAGVSHAQISELRSGKVRAEATLDLHGMTL
                     EPALSQLKTFLLSSRQMGRRCVLVVHGKGTHSEHGAPLREAVLNQLLGSLSGLVHAFA
                     SAAPSDGGEGATYVMLRGGR"
     gene            4723..>5517
                     /gene="glgP"
                     /locus_tag="VFV99_29860"
     CDS             4723..>5517
                     /gene="glgP"
                     /locus_tag="VFV99_29860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5063635.1"
                     /translation="MSSCPRVAYFCMEYGLDPAFPIYAGGLGILAGDHMKSVGDLKVP
                     LTGIGLFWDEGYTRQLIGKGGVVEDHYPKTPRDALRRLDVRIEVTVRGKKVPCRAWQV
                     DRYITSRLLLIEPERDEDRWITQRLYGGGVEDRLAQEILLGVGGVRLLQALGEKIDVY
                     HFNEGHAVFAGLELLRQDVFGGTSLPERLARLRPHVVFTTHTPVPAGNEVHDLDVMRK
                     MDADLGFTDKELELIGGDPFSMTVAGLRLSRLANGVAALHGETARDM"
BASE COUNT          902 a         1749 c         1911 g          955 t
ORIGIN      
        1 agcggtcaga tcgcgctcga tccggcgacc ggcgccatga tcgagggtga cgtcgaggcg
       61 caaacgcgcc gcgtgatcgc gaacctcgcc gcggtgctgg cgtcggcagg cgctagcttc
      121 gcggacgtcg tgaagacgac gatctacctg aagagcatgg gcgatttcac gaccgtgaat
      181 ggcgtctacg gcgagcactt cgtgcatgaa ccgcctgcgc gtgccacggt ggaagccgcc
      241 gcgttgccca agggcgcgct cgtcgagatc gacgcaatcg tagtcttcta ggaggcagtc
      301 atgtcgtcat cgatcgaatc ggttctgacc gagaaccgca gctttgcgcc ctcgaaagag
      361 tttgcggcgg cgtcgcgcgt cggcagcatg gccgactacg agaagctgta tcgccgcgcc
      421 gacgaagatc cggaagggtt ttggggcgag gtagcgggcg agctcgcgtg ggccaagccg
      481 tggcagcgcg tgctcgattg gaagctgccc gacgcgaagt ggttcgtcgg cggccggctc
      541 aacgcgagcg cgagctgcct cgacaggcac gtgacgacgt ggcgcaagaa caaagcggcg
      601 ctgatctggg agggcgagcc gggcgattcg cgcgtactga cgtacggcca gctgcaccgc
      661 gaggtgtgca aagcggcgaa cgcgctgatc gagctcggcg tgtcggcggg cgacttcgtc
      721 gcgatttatt tgccgatgat tcccgaggcc gcgatcgcga tgctcgcgtg cgcgcgtatc
      781 ggggcaccgc acacggtcgt gttcggcggg ttttcgtcgg aggcactcgc ggaccgcatc
      841 aaggactgca aggcgaaggt cgtcatcacg tccgacggcg gctggcggcg cggcgcgatc
      901 gtgccgctca aggagaacgt cgatcgggcg atcgaaggca ccacggtgac gcaggtgctc
      961 gtcgtgaagc gctgcgcgaa tcaggtcgcg atgaagaccg gccgcgacgt ctggtggcac
     1021 gacctcgtcg acagcgcgcc accgcgccac gaggtcgata gcttcgacag cgagcatccg
     1081 ctgttctcgc tctatacgag cggcacgacc ggcaagccga agggcatctt gcacacgacc
     1141 ggcggttacc tgaccggcgc gtattacacg acgaagatgg tgttcgatct gcgcgacgac
     1201 gacatcttct ggtgcacagc cgacatcggc tgggtgaccg ggcatagcta cgtcgtgtac
     1261 gggccgctcg cgaatggcgc gacggtactg atgtacgaag gcgcgccgaa tcagccagga
     1321 ccggatcggt tttggcagat catcgcgaag tacggcgtga cggtgttcta cacggcgccg
     1381 acggcgattc gcgcgttcat gcggtggggc gatgcgtggc ccgcgcagca tgatctgacg
     1441 tcgctgcgac tgctcggctc cgtgggcgag ccgatcaatc ccgaagcgtg gatgtggtac
     1501 cgcaagcaga tcggtggtga tcgctgcccg atcgtcgaca cgtggtggca gaccgagacc
     1561 ggcgcgatca tgatctcgcc gctgcccggc gcgacgccga cgaagcccgg ctcggcgaca
     1621 cggccgctgc ctggcatcgc tgcggatgtc gtgctcaagg acggcacgcc gtgcaaggcg
     1681 aacgaaggcg gcttcctggt gatcaagaag ccgtggccgt cgatgctgcg cacgatcttt
     1741 ggcgaccacg atcggttcgt gaagacgtac ttcggcgagg tgcagggctg ctacttcgcc
     1801 ggcgatggcg cgcgcaaaga cgcggacggc tatttctggg tgatgggccg cgtcgatgac
     1861 gtgatcaacg tggcggggca ccggctgtcg acgatggaag tcgagagcgc gctcgtctcg
     1921 catccgaagg tcgcggaagc agcggtcgtc ggcaggccgg atgatctcaa gggccaggcg
     1981 atcgtcgcgt tcgtgacgcc gcgccagaac ttcgtggcgg acgatgcgct cgcgaaggag
     2041 ctgcgcgagc acgtggtcgg tcagatcggc gcgctcgccc gccccgagga gatccggttt
     2101 gccgaagcgc tgccgaagac acgctcgggc aagatcatgc ggcgtctgct acgcgaggtc
     2161 gcgagcggat cgaagtcgcg cggcgacacg acgacgctcg aggatcaggg cgtgctcgag
     2221 cgtctcgcgc agacgttcgc gcaggacgac taacgctact tgctcgtcga ggtcccgacg
     2281 tcgagctact tgctcgtcga ggtctcgacg tcgagctcgg gatcgaattg cggctcggcg
     2341 atctcgtcgg gcaccgtcac cgcggtttcg ttgatcgcgg ggagcgaggt cgtgggcttg
     2401 ccgaacgtga tcggcgtgcc ggcgggcagg gacgtcggct tcgcggtcgc gacgacgtac
     2461 ttcgcgagca gggcaaccag ctgctcgtgt gccttgcgca cgtagccctt gcggagcggg
     2521 cgcaggctgt tcgtgatgat cgagaacgcg agcgggcgcg tcggatcgag gtcgagcacg
     2581 ccggtcagcg cgatcgcggt cgacagcgta ccggtcttgg cgtggatgtg gccttggatc
     2641 tcgggcacgc ggaagcggtg acggagcgtg ccgtccgtac cggcgatcga cagcgagccg
     2701 agccacgcct tcgcgacggc ctggtcgccg tcctgcgcga agccaccagc cgaacgcacg
     2761 atcgcgacca gctcatgtgg cgtgatccgc gtgcgatacg agaggccaga gccggtatcg
     2821 atcacgaccg agtccttcga caggttcgcg tgacgttgca tccacgcgta catcgcgtcg
     2881 atcgcgacgt ccatcgacgg cgtcgtgcgc cgcgacagcg cggccgcggt catgaccacg
     2941 cgatcggcga gccagttgat gctccacttg ttgacgcgcg tgacgatgtc accgagcgtc
     3001 tgcgattcgt gacgcgcgag ctcgaccggc aggcccttcg agccgacctg atcgccgatg
     3061 aagtcaccga gctcgatcgt gctgatgtcg ccgttgacga cgacctgata cgcacgcagc
     3121 gctgcgcgca gctggtgagc cgcgactgcc gtgcgctcct tggtgtagag cgcgtacatc
     3181 gtctcgccgc ccttgccgat ggtgccgccg atcgtgagct tgatgcgctt gtggccggcg
     3241 gcgtcggtgg tgagctcttg cttgtacgtc agcttggggc gcttgcgcgc gcgcttctcg
     3301 gtcttcgcgg tgacatcgac cgtcacgagg tcgaaacctg ccggcgtcgt cgcggtcggc
     3361 ggctggccgg gctcgcctgc ggtgatggcg atcggtacga ccgcgcggaa gatgccgtcg
     3421 cgcgtcggat cgctgcccga cagcacgttg ccatcgagct gcttcacacc gcgcgcggtg
     3481 agcttctgcg cgaggtcatc gatgtcaccg gccgcgagcg tcggatccca ggtgccgagc
     3541 agatacaggt tgccgtggac gacgccggcc gcatccggct cggggccgag cacgcgcgtc
     3601 gtgtagcgga acgtggggcc gagcagctcg agcgcggtcg cggtcgagac catctttacg
     3661 ttcgatgcag gattgagcgg atcgtccgag ttgaccgcga acagcggctc acccgagcga
     3721 gcgtcagcta cgtagagtcc ggtaatgcca ttacgtagtg gcccgcgtag tagtttttcg
     3781 atctgcttcg cgatgtcttc ttcgagcgtg aggggctctt cgcggcggcc gatcacttcg
     3841 cgggccgggc gcagctcgcc acccttgcca aacgcgaccc gcgcgcgcga cgtcgtcgcg
     3901 gccttcttct tcttgtcctt gtcggcggct ttcgcaacta ccgccttcgc cggctttgcg
     3961 agcaccttcg cggcgcgcgt cttcgacggc tgggccatcg ccacgggcga cgcgagcagc
     4021 atcgccacga tgaggacgag agcgcgtttc atcgcccacc tcgcagcatg acatacgtcg
     4081 cgccttcgcc accgtcgctc ggtgccgccg acgcgaacgc gtggacgaga ccggagagcg
     4141 agcccagcag ttgattcagt acggcctcgc gcagcggcgc accatgttcg gagtgcgtac
     4201 ctttaccgtg cacgacgagc acgcagcggc ggcccatctg gcgcgacgac aagaggaacg
     4261 tcttcagctg cgagagtgcc ggctccagcg tcatgccgtg cagatcgagc gtcgcttccg
     4321 cgcgcacctt gccgctacgc agctcggaga tctgcgcgtg cgagacgccg gcggagcgcg
     4381 ctgcgtaccg cgcagcatcg ccatcgacag tgagcttcac ttctggcggt aactccacca
     4441 cacgtacagg agacggcggt ggcggcggaa cgttcacccg gtcgcggctc tcgaggggcg
     4501 tcaccccacc catggcttcg agaaacagcg actgatcttc gggagtcagc accgcacgct
     4561 tcacgcgcga tccggtggac ttacccttca tcctcccatt gtgtactcgc ggctcggcga
     4621 ttccgccaga aatgttgtta aaggcaacac cgcgagcgag atcgcagctt tgctgcccgt
     4681 cggcttgcat gcgtagtacc tggcaggcca aggttccgct cgatgtcgtc ctgtccccgc
     4741 gttgcgtact tctgcatgga gtacggactc gatccagcct tcccaatcta tgccggcggc
     4801 cttggcatcc tggcaggcga ccacatgaag tcggtcggcg acctcaaggt gcccctaacg
     4861 ggcatcggtc tcttctggga tgagggctac acgcggcagc taatcggtaa gggcggcgtg
     4921 gtcgaggatc actatccgaa gacgccacgc gacgcgctgc gccgtctcga cgtccgcatc
     4981 gaggtcaccg tccgcggcaa gaaggtgccg tgtcgtgcct ggcaggtcga ccgctacatc
     5041 acctcgcggc tgctcttgat cgagcccgag cgtgacgaag atcgctggat cacccagcgg
     5101 ctctacggcg gcggcgtcga ggatcgcctc gcgcaagaga ttctcttggg cgtcggtggc
     5161 gtccgcctgc tgcaggcgct cggcgaaaaa atcgacgtct atcacttcaa cgaaggccac
     5221 gcggtctttg cgggcctcga gctgcttcgc caggatgtct tcggaggcac ctcgctgccg
     5281 gagcgactcg cgcgattgcg tccgcacgtc gtgttcacga cgcatacgcc ggtgcccgcc
     5341 ggcaacgagg tccacgacct cgacgtcatg cgcaagatgg acgccgacct cggcttcacc
     5401 gacaaggagc tcgagctgat cggcggcgat ccgttctcga tgacggtcgc aggcctgcgc
     5461 ctgtcgcgcc tcgcgaatgg cgtcgctgcg ctgcacggcg agactgcgcg cgacatg
//
LOCUS       DASUFX010000739         6957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2064074, whole genome shotgun sequence.
ACCESSION   DASUFX010000739 DASUFX010000000
VERSION     DASUFX010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064074"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFV99_29865"
     CDS             complement(<1..906)
                     /locus_tag="VFV99_29865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063636.1"
                     /translation="MSLWSRLERRLGDLASELVLDEYRDQLNQARQLLAHGDTSAAID
                     SLEALLSVKPDHGQALILLGEARLVSRNPQAAQDAFERALKVRGGDPAALVGMGTALV
                     ETAKYDAAVPVLSRAVAEAGGDRAILADAYRGLGVAWRRRGDLDKAIRELRKAVAEDG
                     DDMDARAALGEALVADGGPYDEATRHLERAVVGANPPALALYGLGRMALLDGSPAMAG
                     ERFARARSIAEQDPTVLGYQIRLDILGAQGDAALAEHDAMRAHGFYLEALQLDPRRAE
                     LHAKIAAAHRSIGNIEASLTSFDRAL"
     gene            complement(1030..1182)
                     /locus_tag="VFV99_29870"
     CDS             complement(1030..1182)
                     /locus_tag="VFV99_29870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063637.1"
                     /translation="MQLWVGSHAVVQLPQWTSLVITLAQTPSHTISPDWHIGSAGLSG
                     VTASIG"
     gene            1229..1726
                     /locus_tag="VFV99_29875"
     CDS             1229..1726
                     /locus_tag="VFV99_29875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063638.1"
                     /translation="MANPTAADGVYTNGTTNTSFYCAYATNTQYEFRVNSYVTTPAGG
                     YSVVRATDFNDANFVRAFIGIYNLDAGFKAADAFSPMNCCMSLLAGTRYSFGGIVPFT
                     GSGTSSYCNQPIAAGRFITFGLSGSYGFTLPSNYFSTNPVTEVTGTCTDNNNAAFYVK
                     RSTTN"
     gene            complement(1723..2739)
                     /locus_tag="VFV99_29880"
     CDS             complement(1723..2739)
                     /locus_tag="VFV99_29880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063639.1"
                     /translation="MQRVLSSVLVVALGACGTSSSTDDLGYGDGFGTPDNPVPADDSR
                     PYAVTSKVDFTVEAILPAQIELAVVTLRSFSENPARTLVTLADQYGVPAVKDLYDVIP
                     GVIKDHLEGWINDEIDKIKIDGKTLPQYAGEVAALAETALTQFALDSEMALKPGAATH
                     TLTGIDLSPAGINVKLPITGLAADILTQKPTIVVEQGGALSIGDQHFGLAYGEYAWQG
                     VNLASTQLFGGDVRMTLGKALNCQALAHTVASKCVLGVCVGHETQLDAICEGGLDAIV
                     NEVHDRIAAFRLDVFRFISGTAQLVDDNQDGVADRMVDGAWDAEMNLGMGLRKAPATF
                     SATR"
     gene            complement(2868..3869)
                     /locus_tag="VFV99_29885"
     CDS             complement(2868..3869)
                     /locus_tag="VFV99_29885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063640.1"
                     /translation="MRVLAAVLVATLAACAADEGPEMWPAGLGTPDNPLPEDDLTYVV
                     ASRIDFTGGGAMPAPVTDAVASMRAFSQNPAKTLLGLADQTQVQQLKSAIGTTLTNSL
                     EGWMNTEIDKARIASKTLRQYATDVVTITETSLTNFYLDSALSMTPAKTTHTLIDLNF
                     RPITVDVVVPMGGFVADKMTQNPTLAVAEGGAMILGDEKFGIAFGDHAWAGINTASTT
                     LYGSGIQTAITTGINCSTLAKNVAAKCSGTSCVGHESQLRAICDGGGAALYGQLRERV
                     VALKLDTIRFTAGTARLVDDHADGIADRIDMGTWQLEMDMGVGLQKLTATFMATAQR"
     gene            complement(3998..5776)
                     /locus_tag="VFV99_29890"
     CDS             complement(3998..5776)
                     /locus_tag="VFV99_29890"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX5063641.1"
                     /translation="MILLEHHKQAKREIIARFQQLADVAEGVGMTTLARDIRNTRIPK
                     LEAERFHLVVLGEFNHGKSTFVNALLGQDVLPTGITPTTASINHVVYADSPRAHVKLL
                     SGNEQGIDPSQLKDWVTVAGGHADDVAYVELGFPSDMLRNNVVLVDTPGVNDLNEQRA
                     EVTYGYVPRADAVVFLLDAGQALKDSEREFLRSRVLESARDRLIFVLGKMDMLSPDER
                     TAVVEYVKKGLAQITTDPVVFPLSARDWAKSKDPNSGMPQLEEYFARFLARDRAQVIL
                     DNAAADAARTASYLENNLGVRMRAYDLDINELEQRIAAVREQLDASKRKLDDLHVRIE
                     ADANSIKNNVELDLEAFAKTFVQALPAQIDSVDADDVKTYLPAFIEDKFKEWAELEGA
                     KLATMLEHLAEEVIAITNENVAAAAATLAERLGPNDTQIDITVDSFKYDVGIYAVGAL
                     GTTVMLFVNALAGGLLTLAAPILAIVLKSKIAGDIRVQAKEKVPAAVLKAAEAMKPHF
                     DKCIDDFAKRLSEFVSNAGNTLYRGISEILDRTMSERRERAGEIGDLRQATSAQIAQV
                     KATTAALRQLREGIWATEDQPIDTGN"
     gene            complement(5787..6674)
                     /locus_tag="VFV99_29895"
     CDS             complement(5787..6674)
                     /locus_tag="VFV99_29895"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5063642.1"
                     /translation="MSVGVDFDFARWIAARRGALEQSAREGAAYAFTSERKFRRNLML
                     ARPVTMALEATTRLWRDVARTELLGTAVKVSDSQYPRVYEAAKAAGAALRVKVPVIFA
                     APSKSFKVKVLGTEDAPHLIVNMELAESLDDSELVAAIAHELGHIQNGHILYTTALHY
                     LNNSAAFFVRWVVQPAVMTLQAWSRRAEVTCDRASLLAVRDLDKTLTSIVRIEMGLDK
                     GSAFNAEEYLRALPDIKKGIGRYAELFRSHPYVPKRVQALRLFAGSALYAQVTGQDPA
                     GKPTLADVDKQCAELISVF"
     gene            complement(6694..>6957)
                     /locus_tag="VFV99_29900"
     CDS             complement(6694..>6957)
                     /locus_tag="VFV99_29900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX5063643.1"
                     /translation="GAHVDLGLAMVIYAGTTIAGALSFLPGGLGVTEGAMSMALVKGA
                     AKLDPSTAVAATLLTRLATLWFAVVIGVAFLALARRRIASRSR"
BASE COUNT         1147 a         2290 c         2244 g         1276 t
ORIGIN      
        1 caacgcacgg tcgaagctcg tgagcgatgc ctcgatattg ccgatcgagc gatgtgctgc
       61 cgcgatcttg gcgtgcagct cggcgcgacg tggatcgagc tggagcgctt cgaggtagaa
      121 gccgtgcgcg cgcatcgcat cgtgctcggc gagcgccgca tcgccctgcg caccgaggat
      181 gtcgagccgg atctggtagc cgagcaccgt cggatcctgt tcggcgatcg aacgcgcgcg
      241 cgcgaatcgc tcacccgcca ttgccggcga gccgtcgagc agtgccatgc gtccgagccc
      301 gtacaacgca agcgccggtg ggttagcgcc gacgacagcg cgctcgaggt ggcgcgtggc
      361 ttcgtcgtac gggccgccgt cggcgacgag cgcttctccg agtgccgcgc gcgcgtccat
      421 gtcgtcgccg tcctcggcca cggctttgcg cagctcgcgg atcgctttat cgaggtcgcc
      481 tcgccgccgc cacgcgacac cgaggccgcg atacgcatct gcgaggatcg cgcggtcgcc
      541 gcccgcttct gctacggcac gagagagcac aggtacggcg gcgtcgtact tcgccgtctc
      601 gacgagcgcg gtgcccatgc ccaccagcgc cgccggatca ccgccgcgca cctttagcgc
      661 acgctcgaac gcgtcctggg cggcttgtgg gttgcgcgaa acgaggcgcg cctcgccgag
      721 caggatcaac gcctgcccgt gatccggttt caccgacagc agcgcttcga gtgagtcgat
      781 tgcggcgctc gtgtcaccat gcgcgagcag ctgccgggcc tggttcaact ggtcacggta
      841 ctcgtcgagg accagctcgc tggcgaggtc gccgagccgc ctttcgagtc gtgaccaaag
      901 tgacatgtgt gcagcctacc attcgatctc gaacccggtt tggccgtatg attgtggaat
      961 gcgaagcttt gtggtggctg tcgccgtact gacttgtgct tgtggtgacg tactcaagac
     1021 tcccgatgct caaccgatcg atgccgtgac gccggatagc ccggccgacc cgatgtgcca
     1081 gtcgggcgag atggtgtgtg acggcgtttg cgccaacgtg atgacgagcg acgtccactg
     1141 tggcaactgc acgacggcat gcgagccgac ccagagctgc atggcaggtt cgtgtgtcga
     1201 ccgtttcgcc gattgcaagg ccgtccgcat ggccaatccg accgcagccg acggcgtcta
     1261 tacgaacgga acgacgaaca cctcgttcta ctgtgcgtac gcgaccaaca cgcagtacga
     1321 gttccgcgtg aattcgtatg tgacgacgcc tgcgggcggc tactcggtag taagagccac
     1381 cgacttcaac gacgcgaact tcgtaagagc gttcatcggc atctacaacc tcgacgccgg
     1441 cttcaaggca gcggatgcgt tctcgccgat gaactgctgc atgagcctgc tcgccgggac
     1501 ccgctactcg ttcggcggca tcgtcccgtt caccggctcc ggcacgagct catactgcaa
     1561 ccagccgatc gcggccggca ggttcatcac gttcggtctc agcggctcct atggtttcac
     1621 gctgccgagc aactacttct cgacgaaccc ggtgaccgaa gtgaccggga cctgcaccga
     1681 taacaacaac gccgcgttct acgtgaagcg ctcgacgacg aactagcgag ttgcggagaa
     1741 cgtagccggc gccttgcgca ggcccatgcc gaggttcatc tcggcgtccc acgcgccgtc
     1801 gaccattcgg tcggcaacgc cgtcctgatt gtcatcgacg agctgcgcgg taccgctgat
     1861 gaagcggaac acgtcgagcc ggaacgcggc gatgcgatcg tggacctcgt tgacgatcgc
     1921 gtcgaggccg ccctcgcaga tcgcatcgag ttgggtctcg tgaccgacgc agacgccgag
     1981 cacgcacttc gacgcgaccg tatgcgcgag cgcctggcag ttcagcgctt tgccgagcgt
     2041 catccgcacg tcgccaccga acagctgcgt gctcgcgaga ttcacgccct gccacgcgta
     2101 ctcgccgtac gcgaggccga agtgctgatc accgatcgag agcgcgccac cttgttcgac
     2161 gacgatcgtc ggcttctgcg tcaggatgtc ggctgcgagg cctgtgatcg ggagcttcac
     2221 gttgatgccg gccggcgaga ggtcgatgcc ggtcagcgtg tgcgtcgcgg cgccgggctt
     2281 gagcgccatc tcgctgtcga gtgcgaactg cgtcagtgcg gtttcggcga gcgccgcgac
     2341 ttcgcccgcg tactgcggca gcgtcttgcc gtcgatcttg atcttgtcga tctcgtcgtt
     2401 gatccagccc tcgaggtgat ccttgatgac gccgggaatg acgtcataga ggtccttcac
     2461 cgcaggcacc ccgtattgat cggcgagtgt gaccagcgtg cgcgccgggt tctccgagaa
     2521 cgagcgcagc gtgacgaccg cgagctcgat ctgcgcgggc aagatcgcct cgaccgtgaa
     2581 gtcgaccttc gacgtcaccg cgtacggccg gctgtcgtcc gccggcaccg ggttgtccgg
     2641 agtgccgaac ccatccccgt agccgaggtc gtccgtcgac gacgacgttc cgcaggcacc
     2701 aagggcgacg accaggacac tggagaggac gcgctgcatg gcccagctcc ttcagcaagg
     2761 ccgcggccag gcgctaaccg cttggatcga cacaagacgt gcaacgtggc cccggagtgg
     2821 cgacccacca cgtgcccacg gtcctctgcg gcctctcgag tagccagtca gcgctgagcg
     2881 gtcgccatga acgtcgcggt cagcttctgc agcccgacgc ccatgtccat ttcgagctgc
     2941 caggtcccca tgtcgatgcg gtcggcaatg ccgtcggcgt ggtcgtcgac gagccgcgcg
     3001 gtcccggccg tgaagcggat cgtgtcgagc ttgagcgcca ccacgcgctc gcgcagctga
     3061 ccgtagagtg cagcgccgcc accgtcgcag atcgcgcgaa gctgactctc gtgaccgacg
     3121 cacgacgtgc cggagcactt cgcagcgacg ttcttcgcga gcgtcgaaca attgatgccg
     3181 gtcgtgatcg ccgtctgaat gccgctgccg tagagcgtcg tgctcgccgt gttgatgccg
     3241 gcccacgcat ggtcgccgaa cgcgatgccg aacttctcgt caccgagtat catcgcgccg
     3301 ccctcggcga ccgcgagcgt cgggttctgc gtcatcttgt cggcgacgaa gccccccatc
     3361 ggcacgacga catcgacggt gatcggccgg aagttgagat cgatcagcgt gtgcgtcgtc
     3421 ttcgccggtg tcatcgacag cgcgctatcg aggtagaagt tcgtcagcga cgtctcggtg
     3481 atcgtcacga catcggtcgc gtactggcgc agcgtcttgc tcgcaatccg cgccttgtcg
     3541 atctcggtgt tcatccaacc ttcgaggctg ttcgtcagcg tcgtaccgat cgcgctcttg
     3601 agctgctgca cctgcgtttg atcggcgagg ccgagcagcg tcttcgccgg attctgcgag
     3661 aacgcccgca tcgatgcgac cgcatcggtg accggagccg gcatcgcacc gccgcccgtg
     3721 aaatcgatcc gcgacgcgac gacgtacgtc agatcgtctt cgggcagcgg attgtcggga
     3781 gtgccgagac ccgcgggcca catttcaggc ccttcgtccg ctgcgcatgc tgcgagggtc
     3841 gcgacgagga cggcagccag cacgcgcatg gtcgcttctc ttcagcacga cccgcgccag
     3901 cgctaaccgc ctggatatac aacgcgagag caacgcaacg ccggtgagga tcctcaccag
     3961 ctggtgccca gcaccatcat cagttggcgc ttcagaatca gttgccagta tcgatgggct
     4021 gatcttcggt ggcccagatg ccttcgcgga gctggcgcaa cgccgccgtg gtcgctttga
     4081 cctgagcgat ctgcgcgctc gtggcctggc gcaaatcgcc gatctcgccg gcgcgctcgc
     4141 ggcgctcgct catcgtgcga tcgagaattt cgctgatgcc gcgatagagc gtgttgcccg
     4201 cgttcgacac gaactcggag agccgcttcg cgaagtcgtc gatgcacttg tcgaagtgcg
     4261 gcttcatcgc ttcggccgct ttgagcacgg ccgccggcac cttctccttc gcctgcaccc
     4321 gaatgtcgcc ggcgatcttc gacttcagca cgatggcgag aatcggcgcg gcgagcgtga
     4381 gtagaccacc ggcgagcgcg ttcacgaaca gcatcaccgt cgtgccgagc gcgccgacgg
     4441 cgtagatgcc gacgtcgtac ttgaacgaat cgaccgtgat gtcgatttgc gtatcgttcg
     4501 ggccgagccg ctcggcgagc gtggcggcag cggcggcgac attctcgttc gtgatcgcga
     4561 tgacctcttc ggcgaggtgc tcgagcatcg tcgcgagctt cgcgccttcg agctctgccc
     4621 actccttgaa cttgtcctcg atgaacgcgg gcaagtaggt cttcacatcg tcggcgtcga
     4681 ccgaatcgat ctgcgccggc agtgcctgca cgaacgtctt cgcgaacgct tcgagatcga
     4741 gctcgacatt gttcttgatc gagttcgcat cggcttcgat ccgcacgtgc agatcgtcga
     4801 gcttgcgctt gctcgcgtcg agttgctcgc gcaccgcggc gatccgctgc tcgagctcgt
     4861 tgatgtcgag atcgtaggcg cgcatccgca cgccgaggtt gttctcgaga tagctcgcgg
     4921 tgcgcgcggc atcggcagct gcattgtcga ggatcacctg cgcgcgatcg cgagccagaa
     4981 agcgcgcgaa gtactcctcc agctgcggca tgccgctgtt cggatccttc gacttcgccc
     5041 aatcgcgcgc cgacagtggg aacacgaccg gatctgtcgt gatctgcgcc aggcccttct
     5101 tcacgtactc gacgacggcc gtgcgctcgt cgggcgacag catgtccatc ttgccgagca
     5161 cgaagatcag ccgatcacgt gcgctctcga gcacgcgcga ccgcaagaac tcgcgctcgc
     5221 tgtccttcag cgcctgaccg gcatcgagca ggaacacgac cgcatcggca cgcggcacgt
     5281 agccgtacgt gacttcggcg cgctgctcgt tgagatcgtt gacgcccggg gtatcgacga
     5341 gaacgacgtt gttgcgcagc atgtcgctcg gaaagccgag ctcgacgtac gcgacgtcat
     5401 cggcgtggcc gcccgcgacg gtgacccagt ccttgagctg gctcggatcg atcccctgct
     5461 cgttgccgct caggagcttg acgtgcgctc gcggcgagtc cgcgtacacg acgtgattga
     5521 tcgatgcggt cgtcggcgtg atgccggtcg gcagcacgtc ctgtccgagc agcgcgttca
     5581 cgaacgtcga cttgccgtgg ttgaactcgc cgagcacgac gaggtggaag cgctccgcct
     5641 cgagcttcgg gattcgcgtg ttgcgaatgt cgcgtgcgag cgtggtcatg ccgacgcctt
     5701 ccgcgacgtc ggcgagctgt tggaagcgcg cgatgatctc gcgcttggct tgcttgtgat
     5761 gctcgagcaa aatcattcgt ctctccctag aacacgctga tcagctcggc gcattgcttg
     5821 tcaacatccg ccaatgtcgg cttgccggca ggatcttggc cggtgacctg cgcgtacagc
     5881 gcggagccgg cgaatagccg cagtgcctgc acgcgtttgg gcacgtacgg gtggctgcgg
     5941 aacagctcgg cgtagcgacc gatgcccttc ttgatatcgg gcaaggcgcg caggtactcc
     6001 tcggcgttga acgccgagcc tttgtcgaga cccatctcga tgcgcacgat cgacgtgagc
     6061 gtcttgtcga gatcgcggac cgcgagcagc gacgcgcgat cgcaggtgac ctcggcgcgg
     6121 cgcgaccacg cttgcagcgt catgaccgcg ggctgtacga cccagcgcac gaagaacgcg
     6181 gccgagttgt tgaggtagtg cagcgcggtc gtatagagga tgtggccgtt ctggatgtgg
     6241 ccgagctcgt gcgcgatcgc ggcaaccagc tcgctatcgt cgagcgattc ggcgagctcc
     6301 atgttgacga tcagatgcgg cgcgtcctcg gtgccgagca ccttcacttt gaacgacttg
     6361 ctcggggccg cgaagatcac cggcaccttc acgcgcagcg cagcgcccgc agccttcgcc
     6421 gcttcgtaca cgcgcgggta ctgtgaatcg ctgaccttca ccgcggtgcc gagcagctcg
     6481 gtgcgcgcga catcacgcca gagccgtgtc gtcgcttcga gtgccatcgt caccggccgc
     6541 gcgagcatga ggttgcgccg gaacttgcgc tcgctcgtga acgcgtatgc cgcgccctca
     6601 cgtgcactct gttccagcgc tccgcgacgt gcggcgatcc accgcgcgaa atcgaagtcg
     6661 acgcctaccg acatgccggc gttatagcat tcgctaccgg gagcggctgg cgatgcgccg
     6721 tcttgcgagc gcgaggaacg cgaccccgat gacgacggca aaccacagcg tggcgagccg
     6781 cgtgagcagc gtcgcggcga cagcggtcga tggatcgagc ttagcggcac ctttgacgag
     6841 tgccatgctc attgcaccct cggtgacacc tagcccaccg ggtaggaacg acagggcgcc
     6901 ggcgatcgtg gtgccggcat agatgaccat tgcgaggccg aggtcgacgt gcgcacc
//
LOCUS       DASUFX010000740        19159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_953185,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000740 DASUFX010000000
VERSION     DASUFX010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19159
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953185"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFV99_29905"
     CDS             complement(<1..579)
                     /locus_tag="VFV99_29905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063644.1"
                     /translation="MRFRVTYGKYSELTADVQQMAHGGILIRVTDAPNLAFETPVELE
                     LVLPDGTTLTTAGKVLQLLAGHGIAVSVSPQFADEARRHAGAGRDNPRGAPARHERVD
                     RTTAAPPPRVQPEEPSNAQKIQIALHGNRDERNAILRDRNRMLHAYVLKNPGLTVDDV
                     LAIAKNPQMGPELFKQIAERPEWLQRSQIAVAL"
     gene            complement(657..1022)
                     /locus_tag="VFV99_29910"
     CDS             complement(657..1022)
                     /locus_tag="VFV99_29910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063645.1"
                     /translation="MRTILALLLATSLAHADPPRYARKQTVVIEVKLSSRVKPTAPTQ
                     PPPTQPTVTATAIIEAEMKTQPIRREQEVILEKLVADTPDDDPDKPDYMFRLAEHYAK
                     QLRFWRLQAIAPTLPTRAR"
     gene            complement(1110..4214)
                     /locus_tag="VFV99_29915"
     CDS             complement(1110..4214)
                     /locus_tag="VFV99_29915"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5063646.1"
                     /translation="MMQITDVFIRRPVLAIAVNLVILVAGLRAIGSLNTRQYPRLDSS
                     TITITTVYVGASSELVRGFVTMPLERVIAAANGIDYIESQSVRGLSTIHVRLKLNFNA
                     AAALADINARVQQVRADLPPESQIPAIQIEPSEAAFAQMYLSFGSKLLAENQVTDYLI
                     RVVQPRLSAIAGVQRAEILGGRTFALRVWLKPDRMAALNISPSQVRAALAANNYLAAA
                     GQTKGQYIQVDITATTDLTTVDDFEKLVIRDRGGTLVRLRDIASVALGAEDYDIDVRF
                     RGEKAVFMGVWVLPTASSVEVIRRVRAEMARVQHDLPTGMEGTIAFDATQYIEDAMHE
                     VVHTLMDTIVIVMIVIFLFLGALRSVAIPVVAIPLSLVGGVFLMQVFGFTLNLLTLLA
                     IVLSVGLVVDDAIVMVENIERHVRSGKSSFQAALDGARELVGPIISITITLAAVYVPI
                     GFQGGLTGALFREFTFTLAGTVMISGIVALTLSPMMASRLIGGRKKSWFARKSDSAFE
                     ALRRGYTRALGATLRARPAVYAVWVALTLAVIPMYMFSQKELAPLEDQGVIFGSVDAP
                     ANSTIELVTTYTTEVNRIFKTTPEFAYSFQLSLPTGGFGGMLVKPWKDRARPIFPIEQ
                     EVNQKLTGVAGIRAPVFLPPALPSAGLFPIELVIASTADHAEIARYVDQLIVEATKSG
                     QFAFPPIPDVRIDEQNVRLVVDRDKVAAMGQSMQQIGSDLAALLGGNFVNRFDFAGRA
                     YKVIPQIERAARLTPDQLVQIHVSGPGNELVPLGAVATLHEGVQPRSLNRLQQLNAIK
                     IQGVPTRSVDGALQVLESAAAKILPPGYVVDYTGESRQLRTEGGKFLPVMGLALLLIF
                     LVLAAQFNSFRDPFVILAGSVPLAIFGALIFTFLKFSGPPGVHFRLTQGWTTTLNIYS
                     EVGLVTLAGLVSKNGILIVQFANQLQLQGKTKLDAIYEAARIRLRPVLMVMIATVAGH
                     FPLTLVSGPGAAARNSIGLVLVGGMTIGTIFTLFVLPSVYVLLARDHTKDRERLGLPK
                     LAPP"
     gene            complement(4211..5407)
                     /locus_tag="VFV99_29920"
     CDS             complement(4211..5407)
                     /locus_tag="VFV99_29920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5063647.1"
                     /translation="MTEQDTPVVRRRRIRWRRGIVVAIGLFIILGGLAGVKFWQISTL
                     IAVGKDMQAKGPPPETVASAVAKTESWPVQLQAVGTVAGVDSVSVSTEASGIVTKLRF
                     ESGDIVKKDQPLVELDSDVEQAQLKAAVARRDLAKIEAGRSRTLVEKGAVPQSELDSN
                     ETALAAAESEIKTIQAQIEHKVVRAPFAGRAGIRTVHVGQFLAAGTAVTTVESIAGLW
                     VDFTLPQEQLAHVKVGTPVRVALRGQPAFDGTVSAVDSTVDPATRSVKLRATLSDKQA
                     PLRSGMFVTVTVVLPTDTTVVVVPATAIVHAPYGDSVFLIEDKIWGSPGMTKTPDGKE
                     VKIARQQFVRLGVARGDFVAISEGVKAGQQVVSIGGFKLRNGSPIVIDNRVQPKFELA
                     PKPENR"
     gene            5585..6766
                     /locus_tag="VFV99_29925"
     CDS             5585..6766
                     /locus_tag="VFV99_29925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5063648.1"
                     /translation="MTDAELIEATRRGESEAFAILVERYQRMVEAIAYSTTRRAALVD
                     DITQDTFVTAWRTIDRLRDVTRLRAWLCSIARNVARNTRRRYRREAPLRDVELAQTPF
                     DDLSDRERDRDVAKALARLPVRYREPLVLFYYEHCTVKEVADALAVGEDAILQRLSRG
                     RRRLGEALATDVEIALERKPSRAVLAACVLALLPFRAASAAPVAAPAASAATTPRWRI
                     ALGVLAAHWRFPAAVLASAAALVVVVAATERSNAIAARSHAQSTPPPSPPEAKPADET
                     NHAKRTPPQLPDDEDDGRVWANISVASTDSAESCARGARGLVSFVLDRDAIRKHDDGQ
                     LYYEPSPEVERTAEAAAARAAESCRGIAKWPELYLRCEGTVFDIMDGNVTCYPYDPFN
                     S"
     gene            complement(6763..8526)
                     /locus_tag="VFV99_29930"
     CDS             complement(6763..8526)
                     /locus_tag="VFV99_29930"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEX5063649.1"
                     /translation="MRWTYGFIVLAFACGPSARDHGSDDTGGECTPAGAHRCTGRTYE
                     SCVDGAWTQEAECPNGCAEGLGCVDACTEAAATHSYMGCEYWAVDLDNAVEVLGSATQ
                     GLTCAQMYGIGKPTMQKVCAIGNGIAGLCDPPNDGCPMSYTCSQKMVCTLDAQHSPFS
                     IVVSNPQTTDVEVTVVAMNNTMVTKTVQAGQVLAIMPQQEGIADQSVDGTGKMQAAYK
                     ITSTLPIIAYQFNPLDNVDVFSNDASLLIPRTAFDSDYLAMSWPTLERRNGIPSQHDY
                     HGYLTVVAWENGTEIEVTPSADVQASATQPALTAGTPTLFTLGAFDTLNLEALGAGDL
                     TGTRVRATSGATVGLFVGHEAAGFGEDAPPDSHNTRGPCCADHLEEMALPTSTWGKSY
                     TIARSEMRTNENDVIRILARNANTTVTFMPEPTSTMSGDCSHLDAGQYCTVRIMHDTA
                     VEASEPVMVGHYLESAIWNGGGNGVPPTTVGNGDPSMSIAVPVEQYRTDYTILVPSSY
                     QQNFLSISTGATGTVTVDGSPLMLDAAVGFRAARTPVAAGQHTIKCPERCGVEVYGYS
                     DAVSYMFAGGLDLRPIVLSTH"
     gene            complement(8652..9209)
                     /locus_tag="VFV99_29935"
     CDS             complement(8652..9209)
                     /locus_tag="VFV99_29935"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5063650.1"
                     /translation="MEAIIVAATRVLEREGLERLTTTRIAEVAGVSVGSLYQYFPNRE
                     AIVGAIIDRQLDTMLRAFRELVTTFAGLALEQCVTSVLFGLLEVARLHEKLHAPLFEE
                     MSAARRSEQHQRALDAYVDIVAATLATRRDVTLACPRAAANLIVHAGDGVIRSLVLTT
                     DVATANVLVREAVRMIMRYLTPAPS"
     gene            complement(9348..11105)
                     /locus_tag="VFV99_29940"
     CDS             complement(9348..11105)
                     /locus_tag="VFV99_29940"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5063651.1"
                     /translation="MARLDPHSYCDDTQAQTESFALTARIDFATQVIAAEVVLTFRAP
                     ASGRLDLDTRDLAIERIQDASGNPLTFQLHEADPFLGARLAIELAAPTPAIRIRYRTS
                     PSASALQWLAPAQTSGGKQPFLFSQCQAIHARSVIPMQDTPRLRVRYTAELVVPRTLH
                     AVMAAADRGREEAGELATHRFEMPQPIPPYLFAFAVGDLVSRDLSPRCRVWAEPAVIE
                     AAAYEFAEVDGMMTAAEALFGPYDWERFDILTMPPSFPYGGMENPRLTFITPTIIAGD
                     RSLVSVVGHELAHSWTGNLVTNANAEHFWLNEGFTTYAERRIVEATEGADMAALQAAL
                     GRRELDESLERFAKRPELTKLRTHLDGVDPDDAYSLVPYEKGYLLLCAIEAVVGRDKF
                     ARWLRSYLDTFRFAAITTDDFIRHIDAGLPGALAAANAEAWLDGPGLPDWVPPFRSSR
                     LDAVEALTGSVPTPAQCAGWSPIEWALYLENVPRPASEQTCRALDEQHRLTASRNYDV
                     LVPWLTLALKSGYAPVLPRVEEVLGAVGRMKYLRPLFTALAADPRTRETATTIFAKVG
                     ASYHPIARQMVESILSGRA"
     gene            complement(11124..11855)
                     /locus_tag="VFV99_29945"
     CDS             complement(11124..11855)
                     /locus_tag="VFV99_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX5063652.1"
                     /translation="MRRTHNEDCYCILEDEALVLVADGMGGHAAGEVASALVTTEVAG
                     FYHQTHKRLPNPWPFECQPQLTELEKRLVCGFKVANLKVFEAANSESGRRGMGTTLVA
                     AALEGDRICISHVGDSRAYRIRDGEIVRLTRDHSLLEQFKEINPEMTREEEDRFPHKN
                     VITRALGLNDFVEVDVKTHDLLPGDIYLLCSDGLSGQLPDEEMLEMISGSTDLAHTVG
                     ELIERANEAGGNDNITAVLVRCARS"
     gene            complement(11937..13739)
                     /locus_tag="VFV99_29950"
     CDS             complement(11937..13739)
                     /locus_tag="VFV99_29950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063653.1"
                     /translation="MTTPRTSALSILAVYWLLLILGMAVCITIRSDAQIAAIWTGTVA
                     GTALGHALALLNTRNWFALLTVLTTAAIVGPSAPGELSASTLWLAFIPAACCAFWSLG
                     DRTTLVAFWFPAVIWMLSILDHARANGIPDDSGIVLLGVLAVLFLLFLRVRETRRVAL
                     WSAVPAPSITLAEPKPPVMLRERPGFRLARAGWALVASSLAFGATAWVAPRLWQSESL
                     AGESTQVAVEHAGPGLPCCPIVDDVETPRARVKEYFAIGRGHDESSSDPIEPPPQSCR
                     RCDVGGTEVAGGRPSLPVTGDLYPYNGPTTYSPNGYVVEGPRQHGTVGYAGTVTTGDP
                     WESDTTSGYLPQDWTPPTVTGRTYTPPVAEGSYVPPDSDGIPHPSIANTPSRAQAIEP
                     APVPQVEQPRYEPPPPPQVAQPPVAPAPAPVAPSPEPPAVQPPHAETATAAAPAPASA
                     TPRRPASTIIGPSLLHWVGVILAAAVLMQLAALALRPIRRLVTLRHLRKPYWEETVDQ
                     RVSNAWQLALVGLRDAGWRPDANESPRDLAQRVGIDGVERCAGILDRARHGIGIDAED
                     LSTMTSSAEAAYQDARSRLGPVARAATWLRWPLT"
     gene            complement(13736..15253)
                     /locus_tag="VFV99_29955"
     CDS             complement(13736..15253)
                     /locus_tag="VFV99_29955"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5063654.1"
                     /translation="MSARAWRIWALFGSSFLMALVTLSARDALVNSAGQILVGMGVTT
                     LGALIILALDARRSQCSVWERIDVLTSAGASMMVSGTVALLLSASVGWASFSIVGVLG
                     IGAVYLAVIWTALVASSDVPWRHATVTRTIVPDRAVEGEPLHEEVRLTNVPIAPGMRL
                     FITGRAMRHGAVSRYTVDSRASRSDVRLESELGPALRGEHRAPPLELWLGDIFGLTRS
                     PVIRRAEATFTVLPRPMQVANAQELLGQGGDAQTSVPAHAIPTEGSFKIREYVDGDDT
                     RRIHWLRSLQQNRLVVRLPDEIPPADPAVRIIIDNELAGTEALTCKAPTEMLDALVRV
                     WLGIGKALADRGTRVTLVAGVGTDTIKPVERVLVARASRSQAIQLGARVTWQEQLPLA
                     SLVDERGERQIVVSCRPRRLPREAKLSWVVVPEVAWTTLETPPGREPTFTYAFPAGSA
                     ENRASRRSAAQRDAHTRWHDLSVFSQVVCWADWPRFAGDHVARRDGDRAVLEVIQ"
     gene            complement(15253..16614)
                     /locus_tag="VFV99_29960"
     CDS             complement(15253..16614)
                     /locus_tag="VFV99_29960"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5063655.1"
                     /translation="MGRLVAGVLRATGSYTERDLQRANDALSALSRPPLGDHDFVSET
                     PEQLAPQIPEHLRAPLLQLAYELAGDEPIRRRLADAYAGLWQFEAAPPVATRNGGAAV
                     IRWLIGKLPHHHLGDSPEEIEMNENGPYRGEPAAPVVAPTERSPLRTRVERIRQQYLQ
                     VVAAVEQVIVGKRNVIERVLAAMAARGHVLLVDVPGVGKTQLCKAIAAAIENRFGRIQ
                     FTPDLLPMDITGANVVDMRDHQMRFRPGPVFTHILLADELNRATPKTQSALLEVMEER
                     CVTVDGVTHHLEEPFQVLATMNPIEHHGTYALPAAQIDRFMVMLEIGYPSPDDEVKVL
                     DYHLGVTPPLAAVKPVISRADFLVWRDTVPYIHVTPELKRTVVDYVSKLRRSGEDGHA
                     ISPRATIAWVRAAQARAMLAGREFVTIEDVLDVAPDVLRHRMWTDAATVRERLRAVVI
                     GAR"
     gene            complement(16672..17514)
                     /locus_tag="VFV99_29965"
     CDS             complement(16672..17514)
                     /locus_tag="VFV99_29965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5063656.1"
                     /translation="MRLVDYAKAHLVVVPIDAIGALIAAGSVTIDGRVGRIDSRVDAT
                     ATVEVEHAALKRILVPEPIPLAIVHEDDDLIVCDKPAGMHVHPLGLHRDGTLLGALLW
                     HCGARPDQPWGRWRPHPLHRLDRAASGLIAIGKRAAVHEQVRQRFAEHAIERRYRAVV
                     HGCVAGETGTIDAPLGRDPTCDYRRAVVPGGQRAVTHWQVLERERDRTWLELTLETGR
                     THQIRAHLASIGHPIVGDTLYATGRNAAAVISLHAALLRMRHPRTGKPVTWTSANKVQ
                     RLDV"
     gene            17683..19005
                     /locus_tag="VFV99_29970"
     CDS             17683..19005
                     /locus_tag="VFV99_29970"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5063657.1"
                     /translation="MLPPGPSSPVLYQIGRWVVQPLELLDDYHARYGDVFSLRWFGGI
                     KFAVVATPELIKQVVSADADVLLAGVGNATLLEPMLGKHSLLTLDGKEHLRQRRLLLP
                     AFHGERMHAFEAAMREITEASFAQWPLGQPFSLHPFMQSITLDVILRTVFGFAHSDDH
                     RELRARLVALLDVAANPWLLFPGMMGIDPLRLPWLRITKLKRALDDSLYRLIAARRRA
                     PGGTDVLAMMLEARDEQGKAMTDVEVRDELITLLLAGHETTATALAWTFDQLLANPQT
                     FDKLQAELAAGSDSYLDCVIRETLRVRPIVPLIGRHVAKPFQLGNWMIPPGHRIAPSI
                     YLSGQNPAVYPEPKRFQPERWIGVKPDPYTWLPFGGGVRRCIGMAFAQFEMRIVLQTV
                     VARARMHLADGPAKVVRRGITLAPSGGTRVVLDERIATRPAPRAVYQA"
BASE COUNT         3226 a         6367 c         6407 g         3159 t
ORIGIN      
        1 gagcgcgacc gcgatctgcg agcgctgaag ccactcgggg cgctccgcga tctgcttgaa
       61 cagctcgggg cccatctgcg ggttcttcgc gatcgcgagc acgtcgtcga cagtgaggcc
      121 cggattcttg agcacgtacg cgtgcagcat gcgattgcga tcgcgcagga tcgcattgcg
      181 ctcgtcgcgg ttgccgtgca acgcgatctg gatcttctgc gcgttgctcg gctcttcggg
      241 ttgtacgcgc gggggcggag ctgcggtcgt gcgatcgacg cgttcatgcc gcgccggcgc
      301 gccgcgcggg ttgtctctgc ccgcacctgc atggcgccgc gcctcgtcgg caaactgcgg
      361 cgaaacactg acggcgatgc cgtgccctgc aagcaactga agcaccttac ccgcggtcgt
      421 cagcgtggtg ccatcgggca ggacaagctc gagctcgacg ggcgtctcga atgcgaggtt
      481 gggagcgtcg gtcacgcgga tgaggattcc gccatgcgcc atctgctgca cgtcagcggt
      541 gagctcgctg tacttgccgt acgtcacgcg aaaccgcatc acgatcatct tagcgcgcct
      601 cgctcgatct gcagtccatt cagcttggaa tgccgccgca gtgagaagcg ctacagctaa
      661 cgcgcgcgcg tcggcagtgt cggggcgatc gcctgcaggc gccagaatcg cagctgcttc
      721 gcgtagtgct cggcgagccg gaacatgtag tcgggcttgt ccggatcgtc gtcgggcgtg
      781 tcggcgacga gcttctcgag gatcacttcc tgctcgcggc ggatcggctg cgtcttcatc
      841 tccgcctcga tgatcgcggt cgcggtgacc gtcggttgcg tcggcggcgg ttgcgtcggc
      901 gcggtcggtt tgactcgcga cgacagcttc acctcgatca cgacggtttg cttgcgcgcg
      961 tagcgcggtg gatctgcgtg agcgagtgac gtcgcgagca agagagcgag gatcgtgcgc
     1021 atgcctagac ggtagcgacc ctgtgcgatc aggtcgtcac cacgtcgcgt gcgcgcgtca
     1081 catctgcgtc gccggttgtc acgacgttgt cagggcggcg cgagcttcgg cagaccgagc
     1141 cgttcgcgat ccttggtgtg atcgcgcgcg agcagcacgt agaccgacgg cagcacgaac
     1201 aacgtgaaga tcgtgccgat cgtcatgccg ccgacgagca cgagaccgat cgagttacgc
     1261 gccgccgcgc cgggaccact gacgagtgtg agcgggaagt gacccgcgac ggtcgcgatc
     1321 atgaccatca ggaccggacg gagccggatc cgtgcggctt cgtagatcgc gtcgagcttg
     1381 gtcttacctt gcagctgcag ctgattcgcg aactggacga tcaggatgcc gttcttcgac
     1441 accagacctg caagcgtcac gaggccgact tcggagtaga tgttgagcgt cgtcgtccag
     1501 ccctgcgtga gccggaagtg cacgcccggt gggcccgaga acttgaggaa cgtgaagatg
     1561 agcgcgccga agatcgcgag cgggaccgag ccggcgagga tcacgaacgg atcgcggaac
     1621 gagttgaact gtgccgcgag caccaagaag atcaggagca gcgcgaggcc catcaccggc
     1681 aggaacttgc cgccctcggt acgtagctgg cgtgactcgc cggtgtagtc gacgacgtag
     1741 cccggcggca ggatcttggc ggccgccgat tcgagcacct ggagcgcgcc gtcaaccgag
     1801 cgcgtcggta cgccttggat cttgatcgcg ttgagctgct gcaagcgatt gagcgagcgc
     1861 ggctggacgc cttcgtgaag cgtggcaacg gcgccgagcg gtacgagctc gttgcccggg
     1921 cccgagacat ggatctgaac gagctggtcg ggcgtgaggc gcgcggcacg ctcgatctgc
     1981 gggatgacct tgtaggcgcg gcctgcgaag tcgaagcggt tgacgaagtt gccgccgaga
     2041 agcgccgcga ggtcgctgcc gatctgctgc atgctctggc ccatcgccgc gaccttgtcg
     2101 cgatcgacga cgagccgaac gttctgctcg tcgatccgga cgtctgggat cggcgggaac
     2161 gcgaactgac cgctcttggt cgcctcgaca atcagctgat cgacatagcg cgcgatctcg
     2221 gcatgatcgg ccgtcgatgc gatcacgagc tcgatcggga acaggcccgc actcggcagc
     2281 gcgggcggca gaaataccgg tgcgcgaatg ccggccacgc cggtcagctt ctggttgacc
     2341 tcttgctcga tcggaaagat cgggcgcgcg cgatccttcc atggtttgac gagcatgccg
     2401 ccgaaaccgc cggtcggcag actgagctgg aagctgtacg cgaactccgg cgtggtcttg
     2461 aagatgcgat tgacctcggt cgtgtaggtc gtaacgagct cgatggtcga gttcgccggc
     2521 gcgtcgacgg agccgaagat cacgccctga tcctcgagag gcgcgagctc cttttgcgag
     2581 aacatgtaca tcggaatgac cgcgagcgtg agcgcgaccc acaccgcgta gaccgccggg
     2641 cgagcgcgca gcgtcgcgcc gagcgcgcgc gtgtaaccac ggcgaagcgc ctcgaacgcc
     2701 gaatcggatt tccgggcgaa ccagcttttc ttgcgcccgc cgatcagccg cgacgccatc
     2761 atcggcgaga gcgtgagcgc gacgatgccg gaaatcatca ccgtgccggc gagcgtgaac
     2821 gtgaactcgc ggaacagcgc gccggtaagt ccgccctgaa agccgatcgg cacgtagacc
     2881 gccgcgagcg tgatcgtgat cgagatgatc gggccgacca gctcgcgcgc accgtcgagg
     2941 gccgcctgaa acgacgactt gccggagcgc acgtggcgct cgatgttctc gaccatgacg
     3001 atcgcgtcgt cgacgacgag accaaccgac agcacgatcg cgagcagcgt gaggaggttc
     3061 agcgtgaagc cgaacacctg catcaagaac acaccgccaa cgagcgagag cggaatcgcg
     3121 accaccggaa tcgcgacgct gcggagcgca ccgaggaaca agaaaatcac gatcatgacg
     3181 atcacgatcg tgtccatcag cgtgtggacg acctcgtgca tcgcgtcctc gatgtattga
     3241 gtcgcgtcga atgcaatcgt gccctccatc ccggtcggta ggtcatgctg gacgcgcgcc
     3301 atctcggcgc gaacgcgacg aatgacctcg acggacgacg cggtcggcag cacccacacg
     3361 cccatgaaca cggccttttc gccgcggaac cgcacgtcga tgtcgtagtc ctcggccccg
     3421 agcgcgacgc tcgcgatgtc acgcaggcga acgagcgtgc cgccgcgatc gcgaatgacg
     3481 agcttctcga aatcgtcgac ggtcgtcaga tcggttgtcg ccgtgatgtc gacctggatg
     3541 tactggccct tcgtctggcc ggcggcggcg aggtagttgt tcgcggcgag cgcggcacgc
     3601 acctgcgacg ggctgatgtt cagcgccgcc atgcggtcgg gcttgagcca cacgcgcagc
     3661 gcgaacgtgc ggccaccgag aatctcggca cgctgcacgc cggcgatcgc ggacaggcgc
     3721 ggctgcacga ctcggatcaa gtagtcggtg acctggttct ccgcgagcag cttcgagccg
     3781 aagctgaggt acatctgcgc gaacgccgct tccgacggct cgatctggat cgcgggaatc
     3841 tgtgactcgg gcggcaggtc ggcgcgcacc tgctgcacgc gcgcgttgat gtcagcaagc
     3901 gcagcagccg cgttgaagtt gagcttgagg cgcacgtgga tcgtcgacaa tccgcgcacg
     3961 ctctgcgact cgatgtagtc gatgccgttg gctgccgcga tcacccgttc gagcggcatc
     4021 gtcacgaagc cacggacgag ctccgagctc gcaccgacgt agaccgtggt gatggtgatc
     4081 gtcgagctgt cgagacgcgg atactggcgc gtgttgagcg aaccgatcgc acgcaggccg
     4141 gcgacgagga tcacgaggtt caccgcgatc gcgagtaccg gccggcggat gaagacgtcg
     4201 gtgatctgca tcatcgattc tcgggcttcg gcgcaagctc gaacttcggc tgcacgcggt
     4261 tgtcgatcac gatcggagag ccgttgcgaa gcttgaagcc gccgatgctg acgacctgtt
     4321 ggcctgcctt cacgccctcg ctgatcgcga cgaagtcacc gcgcgccaca ccaaggcgca
     4381 cgaactgctg gcgcgcgatc ttcacctcct taccgtcggg cgtcttcgtc attcccggcg
     4441 agccccagat cttgtcctcg atcaggaata ccgagtcccc gtacggtgcg tgaacgatcg
     4501 cagtcgcggg caccacgacg accgtcgtgt ccgtcggcag cacgaccgtg acggtcacga
     4561 acatgccgga ccgaagcggc gcttgcttgt cgctcagcgt cgcgcgcagc ttcacgcttc
     4621 gcgtggccgg atcgactgtc gagtcgaccg cgctgacggt gccgtcgaac gcgggttggc
     4681 cgcgcagcgc gacgcgcacc ggtgttccga ccttcacgtg cgcgagctgc tcctgcggta
     4741 gcgtgaaatc gacccacaga cccgcgatcg actcgactgt cgttaccgcg gtgcccgcag
     4801 cgagaaactg gccgacatga acggtgcgaa taccggctcg gccggcaaac ggtgcacgta
     4861 cgaccttgtg ctcgatctgc gcctggatgg tcttgatctc gctctccgca gcggcgaggg
     4921 cggtctcgtt gctgtcgagc tccgactgcg gaaccgcacc tttttcgacg agcgtacgcg
     4981 accgcccggc ttcgatcttc gcgagatcgc ggcgcgcgac cgccgccttg agctgcgcct
     5041 gctcgacgtc actgtcgagc tcgaccagcg gttggtcctt cttgacgatg tcgcccgatt
     5101 cgaagcgcag cttggtgacg atgcccgacg cctccgtcga caccgaaacg ctgtcaacgc
     5161 ccgccaccgt gccgaccgcc tggagctgga ccggccacga ttcggtcttc gcaactgcag
     5221 aggcgaccgt ctcgggcggc gggccttttg cctgcatgtc cttgccaacc gcgatcaacg
     5281 tcgagatctg ccagaacttc acgccggcca gaccgccgag gatgatgaac aggccaatcg
     5341 caaccacgat gccccggcgc caacggatgc ggcgtcggcg cacgactggc gtgtcctgct
     5401 cggtcacggt cacgaaactt cgtgcacgcc ggaagccagc cacgcacggc gtacatgcgc
     5461 gatttccagc acgactgcgc agttagaacg cgcagcgcgg cggcgcagcg tgcgcacacg
     5521 acgtactccg atccccgtac atttcttgtc aggttttggg cggctcgggt tactaccgcg
     5581 cgagatgacc gatgccgagc tgatcgaggc gacccgccgc ggcgaaagcg aggcgttcgc
     5641 tatcctggtc gagcgctacc agcgcatggt ggaggcaatc gcgtacagca cgactcggcg
     5701 ggccgcgctg gtcgatgaca tcacccagga cacgttcgtg acagcgtggc gaacgatcga
     5761 ccgactgcgt gacgtgacgc gactacgcgc gtggctgtgt tcgatcgccc gcaacgtcgc
     5821 gcgcaacacc cgccgccgct accgccgcga ggcgccgcta cgcgacgtcg agctcgcgca
     5881 gacgccgttc gatgatctga gcgatcgcga gcgcgaccgc gatgtcgcca aagcgctcgc
     5941 tcgactcccc gtgcggtacc gcgaaccgct cgtgctgttc tattacgagc actgcacggt
     6001 gaaagaagtc gccgatgcgc tcgcggtcgg cgaggacgcg atcctgcagc gcctttcgcg
     6061 cggccgccgg cgactgggcg aagcgctcgc cacggatgtc gagatcgcgc tcgagcgcaa
     6121 accatcgcgc gcggtgctcg cagcttgcgt gcttgcactc ttgccgttcc gcgccgcctc
     6181 cgccgcgccg gtcgcagcac ccgcagcatc ggcagcaacc acgccgcggt ggcggatcgc
     6241 gctcggcgtg cttgccgcgc actggcgctt tcccgccgcg gtgctcgcga gcgcagcggc
     6301 actcgtcgtg gtggtcgcag cgaccgagcg tagcaacgcg atcgcagcac gcagccacgc
     6361 gcagagcacg ccgccgccct ccccgcccga agcgaagccg gccgatgaaa ccaaccacgc
     6421 gaagcgaacg cctccccaac ttcccgacga cgaagacgac ggtcgcgtct gggcgaacat
     6481 ctcggttgcg agcactgatt cggcagagag ctgtgcgcgc ggtgcgcgag gcctggtctc
     6541 gttcgtgctc gaccgggatg cgattcgcaa acacgacgac ggtcagctct attacgagcc
     6601 gtcgcccgag gtagagcgca cggcggaggc cgctgccgcg cgtgccgccg agagctgccg
     6661 cggcatcgcg aagtggcccg agctgtactt gcggtgcgaa ggcaccgtgt tcgacatcat
     6721 ggacggcaac gtcacctgct atccgtacga tccgttcaac agctagtgcg tcgacaacac
     6781 gatcggtcgt agatcgagac cgccggcaaa catgtacgag acagcatcgg agtagccgta
     6841 aacctcgacg ccgcagcgct cggggcactt gatcgtgtgt tgaccggcag cgaccggcgt
     6901 gcgcgccgcg cggaagccca ccgctgcgtc gagcatcagc ggtgacccat cgacggtcac
     6961 cgtgccggtc gcgccggtcg aaatcgagag gaagttctgc tggtacgagc tcggcaccag
     7021 gatcgtgtag tcggtgcggt actgctcgac gggcacggcg atcgacatcg acggatcacc
     7081 attgccgacc gtcgtgggcg gcacgccgtt gccgccacca ttccagattg cagactccaa
     7141 gtaatggccg accatgaccg gctcgctcgc ttcgaccgcg gtgtcgtgca tgatgcgcac
     7201 ggtgcaatac tgccccgcgt cgaggtgcga gcaatcgcca ctcatcgtcg atgtcggctc
     7261 gggcatgaac gtcacggtgg tgttcgcatt gcgcgcgagg atgcggatca cgtcgttctc
     7321 gttcgtgcgc atctcgctgc gcgcgatcgt gtagctcttg ccccacgtcg acgtcggcaa
     7381 cgccatctcc tcgaggtgat cggcgcagca cgggccgcgc gtgttgtgcg agtcgggagg
     7441 cgcgtcctcg ccgaacccgg cggcttcgtg accgacgaac aggccaaccg tcgcgccgct
     7501 cgtcgcgcgg acacgcgtac cggtgaggtc gccggctccg agcgcttcga ggttcagcgt
     7561 gtcgaatgcc ccgagcgtga acagtgtcgg cgtgccagca gtgagcgcag gttgcgtcgc
     7621 gcttgcctgc acgtccgcgc tcggcgtgac ctcgatctcc gtgccgttct cccacgcgac
     7681 gacggtgaga tagccgtggt agtcgtgctg cgatggaatc ccgttgcggc gctcgagcgt
     7741 cggccagctc atggcgaggt agtcgctatc gaacgcggtg cgcgggatga gcagcgacgc
     7801 gtcgttcgag aagacgtcga cgttgtcgag tggattgaac tgatacgcga tgatcggcag
     7861 cgtcgacgtg atcttgtatg ccgcctgcat cttgccggtg ccgtcgaccg actgatcggc
     7921 gatgccctct tgctgcggca tgatcgcgag cacctggccc gcctgcaccg tcttcgtgac
     7981 catcgtgttg ttcatcgcga cgaccgtaac ctcgacgtcg gtggtctgcg ggttcgacac
     8041 gacgatcgag aacggcgagt gctgcgcgtc gagcgtgcac accattttct gcgagcacgt
     8101 atagctcatc gggcagccat cgttcggcgg atcgcacagg ccggcgatgc cattcccgat
     8161 cgcgcagacc ttctgcatcg tcggcttgcc gatgccatac atctgcgcgc acgtcaggcc
     8221 ctgggttgcc gagccgagca cctcgacggc gttatcgaga tcgacggccc agtattcgca
     8281 tcccatgtag ctatgggttg cggccgcttc ggtgcacgca tcgacacaac cgagaccctc
     8341 ggcgcagccg ttcgggcact cggcttcctg tgtccacgcg ccgtcgacgc acgattcgta
     8401 cgtgcgcccg gtgcagcgat gcgctccggc cggcgtgcac tcgcctccgg tgtcgtcgga
     8461 gccgtggtca cgcgcgctcg gtccgcatgc gaacgccaac acaatgaagc cgtaggtcca
     8521 gcgcatctcc cctccgttgc gccatccata gcgcgcgaac acaccgttcg tgaattcgca
     8581 tctgtgcgct gctcaactcg gcgaaccagc gctgctatcg cgccgtcaag ccgcagtgct
     8641 tcgcttctcg ctcaacttgg cgcgggcgtc aggtagcgca tgatcatgcg caccgcttct
     8701 ctgacgagca catttgccgt cgcgacgtcc gtcgtcaaca cgagcgatcg gatcacgcca
     8761 tcacctgcgt gaacgatcag gttcgccgcc gcacgtggac atgcgagcgt cacgtcgcgc
     8821 cgcgtcgcga gtgtcgccgc gacgatgtcg acgtaggcat cgagcgcgcg ctgatgctgc
     8881 tcgctccgac gcgccgccga catctcttcg aacagtggcg cgtgcagctt ctcgtgcagg
     8941 cgcgcgactt ccagtaagcc gaacaacacg ctcgtgacgc attgttcgag tgcgagcccc
     9001 gcaaacgtcg tgacgagctc gcgaaacgca cgcagcatcg tgtcgagctg gcgatcgatg
     9061 atcgcgccga cgatcgcctc gcgattcggg aagtactgat acagcgatcc gacgctgaca
     9121 ccggcgacct ccgcgatcct cgtcgtggta aggcgctcga gaccttcgcg ctcgaggacg
     9181 cgcgtggcgg cgacgatgat cgcttccatc gtgatgcgcg cccgttcttg gtgaggctcc
     9241 ttccggagat gaggtggacg gcgctgcatc gacgcgcgca acctagaacg ggtccggtgg
     9301 tccggccgct atccggcact cacgggtgct cgcacgaacc cgccacacta cgcacgacca
     9361 gacaggatcg actcgaccat ctggcgcgcg atcgggtgat agctggcccc gaccttcgca
     9421 aagatcgtcg tcgcagtttc acgcgtgcgc gggtcggcgg cgagcgccgt gaacagcggc
     9481 cgtagatact tcatacgacc gacggcgccg agcacttctt cgacccgcgg cagcaccggc
     9541 gcgtagccac tcttgagcgc gagcgtgagc cacggcacga gcacgtcgta gttgcgcgat
     9601 gcagtcaggc gatgctgttc gtcgagcgcg cggcaggttt gctcggacgc gggccgcggc
     9661 acgttctcga gatagagcgc ccattcgatc ggcgaccagc ccgcgcactg cgctggcgtc
     9721 ggcacgctgc cggtgagcgc ctcgactgca tcgagccgcg acgagcggaa tggcggcacc
     9781 cagtcgggca ggcccgggcc gtcgagccac gcctctgcgt tcgcggcggc gagcgcaccc
     9841 ggcaatcccg cgtcgatgtg gcggatgaag tcgtcggtcg tgatcgccgc gaaccgaaac
     9901 gtatcgaggt agctgcgcag ccagcgcgcg aacttgtcgc ggccgacgac tgcctcgatc
     9961 gcgcagagca gcaggtagcc tttctcgtac gggacgagcg agtacgcgtc gtcggggtcg
    10021 acgccgtcga ggtgcgtgcg cagcttcgtc agctccgggc gcttcgcgaa ccgctcgagg
    10081 gattcgtcga gctcgcggcg cccgagtgcc gcttgcagcg cggccatgtc cgcgccttcc
    10141 gtcgcctcga cgatgcggcg ctccgcgtac gtcgtgaagc cttcgttgag ccagaagtgc
    10201 tcggcgttcg cgttcgtgac gagattgccc gtccacgagt gcgcgagctc gtgaccgacg
    10261 accgacacca gcgaccgatc gcccgcgatg atcgtcggcg tgatgaacgt gaggcgtggg
    10321 ttctccatgc cgccataagg aaacgacggc ggcatcgtga ggatgtcgaa gcgctcccag
    10381 tcataggggc cgaacagcgc ttcggccgca gtcatcatcc cgtcgacctc ggcgaactcg
    10441 tacgcggcgg cctcgatgac ggcgggctcg gcccagacgc ggcagcgcgg cgacaagtcg
    10501 cgcgagacca ggtcaccgac agcgaacgcg aacaggtagg gcggaatcgg ctgcggcatc
    10561 tcgaaccgat gggtcgccag ctcgccggct tcctcgcggc cgcggtccgc cgctgccatc
    10621 acggcgtgga gcgtgcgcgg cacgaccagc tcggccgtgt agcgcacgcg cagccgcggc
    10681 gtgtcctgca tcggaatgac cgagcgcgca tgaatcgcct ggcactgcga gaacaagaat
    10741 ggctgcttgc cgccgctggt ctgtgccggg gcgagccact gcagcgcaga ggcggacggc
    10801 gacgtgcgat agcgaatacg gatcgcgggt gtcggcgctg caagctcgat cgcgaggcgc
    10861 gcgccgagaa acggatctgc ctcgtggagc tggaacgtga gcggattccc gctcgcatcc
    10921 tggatgcgct cgatcgcgag atcgcgagtg tcgagatcga ggcgcccgct cgccggcgca
    10981 cggaacgtga gcacgacctc cgccgcgatc acctgggtcg cgaaatcgat ccgcgcggtc
    11041 agcgcgaacg attcggtctg ggcctgcgtg tcatcgcaat acgaatgcgg atcgagccga
    11101 gccatcggcc ggagaataca tgcctaactg cgcgcgcacc tgaccagaac ggccgtgatg
    11161 ttgtcgttac caccggcttc gttggcacgc tcgatgagct cgccgacggt atgcgcgagg
    11221 tccgtgctgc ccgaaatcat ctcgagcatt tcctcgtcag ggagctggcc ggacaggccg
    11281 tcactgcaca acaagtagat gtcgcccggg agcaggtcgt gcgtcttgac gtcgacctcg
    11341 acgaaatcgt tgaggccgag cgcgcgcgtg atcacgttct tgtgcgggaa gcgatcctct
    11401 tcctcgcgcg tcatctcggg attgatttct ttgaactgct cgagcagtga gtggtcgcgc
    11461 gtgagccgca cgatctcgcc gtcgcggatg cggtatgcgc gcgaatcgcc gacgtgcgag
    11521 atgcagattc gatcgccttc gagcgcggcg gctaccagcg tcgtccccat accgcggcgg
    11581 cccgattcgc tgttcgccgc ttcgaacacc ttcaggttcg cgaccttgaa cccgcacacg
    11641 agccgcttct cgagctcggt cagctgcggc tggcactcga acggccacgg gtttggcagg
    11701 cgcttgtgcg tctggtgata gaagccggcg acctcggttg tcaccagcgc agacgcgacc
    11761 tcgcccgccg cgtgaccacc catgccatcg gcgacgagca cgagcgcttc atcttcgaga
    11821 atgcagtagc agtcctcgtt atgcgtacga cgcattccca ggtcggttat ggccgcatag
    11881 cgggtccgca tctccaccgt gcggagatca atttaccagc gtatttggcg gacgatctac
    11941 gttagcggcc agcgcagcca ggtagccgcc cgtgccacag gtcctagccg gctgcgcgca
    12001 tcctggtacg cagcttctgc agaggatgtc atcgtcgaca ggtcctcggc atcgatgccg
    12061 ataccgtggc gggcacggtc aagaataccc gcacaccgct cgacgccgtc gataccgacc
    12121 cgctgagcca ggtcgcgcgg cgactcgttc gcatcgggac gccaacccgc atcacgcaga
    12181 ccgaccagcg ccagttgcca cgcgttcgac acccgctgat cgacggtctc ttcccagtac
    12241 ggcttgcgca gatggcgcaa cgtgaccagg cgacggatcg ggcgtagcgc aagcgcagcg
    12301 agctgcatca gcaccgcggc tgccaggatc acgcccaccc agtgcagcag cgacggaccg
    12361 atgatcgtcg aggccggccg gcgcggcgtc gccgacgccg gtgcaggcgc ggcggcggtt
    12421 gcggtctcgg cgtgcggcgg ctgcactgcg ggcggctcgg gagatggcgc tacgggagcc
    12481 ggggctggcg caaccggcgg ctgcgcgacc tgcggcggcg gcggtggctc gtatcgcggc
    12541 tgctcgacct gcggtaccgg tgccggctcg atcgcttgcg cgcgactcgg cgtgttggcg
    12601 atcgaggggt gaggaatgcc atcgctgtca ggcggaacgt aggagccctc ggcgaccggc
    12661 ggcgtgtacg tacgtcccgt gacggtcggc ggagtccaat cctgggggag gtaccccgac
    12721 gtcgtgtccg actcccacgg atcacccgtc gtgaccgtgc cggcatagcc gacggtgccg
    12781 tgttggcgcg gcccctcgac gacgtacccg ttagggctgt acgtggtcgg gccgttgtac
    12841 ggatagagat cgccggtgac gggcagactc ggacgtccgc ccgcgacctc ggtgccgccg
    12901 acatcgcagc gccggcagct ctggggcggc ggctcgatcg ggtcactcga gctttcgtca
    12961 tgaccgcggc cgatcgcgaa gtactccttc acgcgcgcgc gcggcgtctc gacatcgtcg
    13021 acgatcggac agcagggcag cccggggccg gcgtgctcga ccgcgacctg agtcgactca
    13081 cccgcgagcg actccgactg ccagagccgt ggtgccaccc acgcggtggc gccgaacgcg
    13141 agtgagctcg cgacgagcgc ccagcccgcg cgcgccagcc ggaagcccgg gcgctcgcgg
    13201 agcatcaccg gcggctttgg ctcggccagc gtgatcgatg gcgccggcac cgccgaccat
    13261 aacgcgacgc gccgggtctc gcgcacacgc aagaacaaga ggaacaacac ggcgagcacg
    13321 ccgagcagca cgatgccgct atcgtcgggg atgccgttcg cgcgcgcgtg atcgaggatc
    13381 gatagcatcc agatcacggc cgggaaccag aacgcgacga gcgtcgtgcg atcgccaaga
    13441 ctccagaacg cgcagcaggc agcaggaatg aacgccagcc acagcgtcga cgccgacagc
    13501 tcgccaggcg ccgatggccc cacgattgcg gccgtcgtca aaacggtgag cagtgcgaac
    13561 cagttccgcg tgttgagcaa cgcgagtgcg tggccgagcg cggtgccagc caccgtgccg
    13621 gtccagatcg cggcgatctg tgcgtcgctg cggatcgtga tgcagaccgc cataccgagg
    13681 atcagcagta gccagtagac ggcgaggatc gagagcgccg aggtgcgtgg ggtcgtcatt
    13741 ggatcacctc cagcaccgcg cgatcaccat cgcggcgcgc gacgtggtcg ccggcgaacc
    13801 gcggccagtc tgcccagcac acgacctgac tgaataccga gaggtcgtgc catcgcgtat
    13861 gcgcgtcgcg ctgcgccgcc gaccggcgcg acgctcggtt ctcggcgctg cccgccggga
    13921 acgcgtacgt gaatgtcggc tcgcgaccag gcggcgtctc gagcgtggtc cacgcaactt
    13981 ccggaaccac gacccacgac agcttcgcct cgcgcggaag ccggcgcggt cgacacgaca
    14041 cgacgatctg gcgttcgccg cgttcgtcga cgagcgacgc gagcggcaac tgttcctgcc
    14101 acgtcactcg cgcgccgagc tggatcgcct gggagcgcga tgcacgcgcg acgagcacgc
    14161 gctcgaccgg cttgatcgta tcggtgccga caccggcgac cagcgtgacg cgcgtgccgc
    14221 gatcggcgag cgccttgccg atgccgagcc acacccgcac gagcgcgtcg agcatctctg
    14281 tcggcgcctt gcacgtgagc gcttcggtgc cggcgagctc gttgtcgatg atgatgcgca
    14341 ccgccggatc tgccggcgga atctcgtcgg gcaagcgaac gacgaggcgg ttctgttgca
    14401 gcgatcgaag ccagtggatg cggcgcgtgt cgtcgccgtc gacgtattcg cggatcttga
    14461 acgagccctc ggtcggaatc gcgtgcgcag gaactgacgt ctgggcgtcg ccaccctggc
    14521 cgagcagctc ctgcgcgttc gcgacctgca tcgggcgcgg cagcaccgtg aacgtcgcct
    14581 cggcacggcg aatcaccgga ctgcgagtga gcccgaagat gtcgccgagc cacaactcga
    14641 gcggcggcgc acgatgctcg ccgcgcagtg cgggaccgag ctcgctctcg agccgaacgt
    14701 ctgaacgcga ggcacgtgaa tcgacggtgt agcgcgagac cgcgccgtgt cgcatcgcac
    14761 ggccggtgat gaagaggcgc atgcccggcg cgatcggtac gttcgtgagc cgcacttcct
    14821 cgtgtagcgg ctcgccctcg acggcgcgat ccggaacgat cgtgcgcgtg accgtcgcgt
    14881 ggcgccacgg cacgtcactg ctcgcgacca aggcggtcca gatcaccgcg aggtacaccg
    14941 caccgatacc gagcacaccg acgatgctga agctcgccca gccgacgctg gcggagagca
    15001 gcaacgcgac ggtgccactc accatcatcg aagcgcccgc cgacgtcagc acgtcgatgc
    15061 gttcccagac cgagcactga ctgcgccgcg catcgagcgc gagaatgatc agcgcaccga
    15121 gcgtcgtgac gcccatgccg acgaggatct ggccggcgct gttgacgagc gcatcgcgcg
    15181 cgctgagcgt caccaacgcc atcaggaacg acgatccgaa cagcgcccag attcgccacg
    15241 cccgcgccga catcaccgtg ctccgatcac gacggcacgc aaccgttcgc gcacggttgc
    15301 agcgtcggtc cacatgcggt ggcggagcac gtcgggcgcg acatccagca cgtcctcgat
    15361 cgtcacgaac tcgcggcccg cgagcatcgc gcgcgcctgc gcggcacgta cccacgcgat
    15421 tgtcgcgcgc gggctgatcg catgaccgtc ttccccggaa cgacgcagct tcgagacgta
    15481 gtcgacgacg gtgcgcttga gctccggcgt cacgtggatg tacggcacgg tgtcgcgcca
    15541 cacgaggaaa tcagcgcgcg agatcaccgg cttcacggcg gcgagcggcg gcgtcacgcc
    15601 gaggtgatag tcgaggactt tgacttcgtc gtccggagac ggatagccga tctcgagcat
    15661 caccatgaag cgatcgatct gcgcggccgg caacgcgtac gtgccgtggt gctcgatcgg
    15721 gttcatcgtc gcgagcacct ggaacggctc ttcgaggtga tgcgtgactc catcgacggt
    15781 aacgcaacgc tcctccatca cctcgaggag cgctgactgc gtcttcggcg tcgcacgatt
    15841 gagctcgtcg gcgaggagga tgtgcgtgaa caccggtcct ggccggaacc gcatctggtg
    15901 gtcgcgcatg tcgacgacgt tggcgccggt gatgtccatc ggcagcagat cgggcgtgaa
    15961 ctggatgcga ccgaatcgat tctcgatcgc ggccgcgatc gccttgcaca gctgcgtctt
    16021 gccaacgccc gggacgtcga ccaggagcac gtggccgcgg gccgccatcg ctgcgagcac
    16081 gcgctcgatg acgttgcgct tgccgacgat cacctgctcc actgccgcga ccacctgcag
    16141 gtactgctgg cggatgcgct cgacgcgcgt gcgcagcggc gatcgttcgg tgggcgcaac
    16201 tacgggggct gcgggttcgc cgcggtacgg accgttctcg ttcatctcga tctcctcggg
    16261 tgaatcgccc aggtgatgat gcggaagctt gccgatgagc cagcggatca cggcggcacc
    16321 gccgttgcgc gtcgcgaccg gcggcgcggc ctcgaactgc cacagcccgg cgtaggcatc
    16381 tgcgagccgg cgccttatcg gctcgtcgcc ggccagctcg tacgcgagct gcaacagcgg
    16441 agcacgcagg tgctcgggaa tctgtggtgc gagctgttcg ggcgtctcgc tcacgaagtc
    16501 gtgatcgcca agcggcggtc gcgacagcgc cgacaaggcg tcgttcgcgc gctggaggtc
    16561 gcgctcggtg taactgccgg tcgcgcgcaa tacgcccgcg acgagtcgtc ccacggcgat
    16621 catgctggcc aaagcctgat atggcggccg gtcacatgcc atgagaaaca actaaacgtc
    16681 gagtcgttgc accttattcg cggacgtcca cgtgacgggt ttgccggtgc gcggatggcg
    16741 catccgcagc agcgccgcat ggagcgagat cactgccgcg gcattccggc cggttgcata
    16801 gagcgtgtcc cctacgatcg ggtgaccgat gctcgcgagg tgggcgcgga tctggtgggt
    16861 acggccggtt tcgagggtca gctcgagcca ggtccgatcg cgctcccgct cgaggacctg
    16921 ccagtgtgtc accgcgcgtt gcccacctgg cacgaccgcg cgccggtagt cgcaggtggg
    16981 gtcgcggcca agcggtgcgt cgatcgttcc ggtctcgccg gcaacgcaac catgcacgac
    17041 agcgcgatag cggcgctcga tcgcgtgctc cgcgaaccgc tgccgcacct gttcgtgaac
    17101 cgcagcgcgc ttgccgatcg cgatgagccc actcgccgca cgatcgagcc gatgtaacgg
    17161 atgcggtcgc caccgtcccc acggctgatc cggacgagca ccgcagtgcc agagcagcgc
    17221 gcccagcaac gtgccgtcgc ggtgcagccc gagcggatgc acgtgcatgc cggccggttt
    17281 gtcacacacg atcaggtcgt cgtcttcgtg aacgatcgcg agtggaatcg gttcgggaac
    17341 cagtatgcgt ttgagtgccg cgtgctcgac ctcgaccgtg gctgtcgcgt cgacgcgcga
    17401 atcgatgcga ccgacacgcc catcgatcgt cacggacccg gctgcgatca acgcaccgat
    17461 tgcgtcgatg ggaacgacga cgagatgtgc tttcgcgtaa tcgacgagcc tcacggattg
    17521 gacgccaatg gtatcgacaa ctagcgcgag cgacggtccc ggcgagcgat ttgcatcgtc
    17581 tctcgacgga ggcccatgta cttcatcatg cttgccacgg ccatcgccac ggtggtcatc
    17641 gcggtcctga ttacgaagcg ctcgtaggcg taagatacgc gggtgctacc gccaggaccg
    17701 agctcgccgg tgctctatca gatcgggcgc tgggtcgtgc agccgctcga gctgctggac
    17761 gactatcacg cgcgctacgg cgacgtgttc tcgttgcgct ggttcggcgg catcaagttc
    17821 gcggtcgtcg cgacgccgga gctgatcaag caagtggtct ccgcggatgc cgacgtcctg
    17881 cttgcgggtg ttggcaatgc gacgctactc gagccgatgc tcggcaaaca ctcgctgctg
    17941 acgctcgacg gcaaagagca cttgcgccag cgacggctgc tgttgcctgc attccacggc
    18001 gaacgcatgc acgcgttcga agccgcgatg cgcgagatca cggaggcctc gttcgcccag
    18061 tggccgcttg gccagccgtt ctcgctgcac ccgttcatgc agtcgatcac gctcgatgtg
    18121 atcttacgga cggtgttcgg ttttgcgcac tcggacgacc atcgcgagct gcgcgctcgg
    18181 ctcgtcgcgc tgctcgacgt cgcggcgaat ccctggctgc tgtttccggg catgatgggc
    18241 atcgatccgc tccgcctgcc gtggctgcgc atcacgaagc tcaagcgcgc actcgacgat
    18301 tcgctgtacc gcctcatcgc ggcacgtcgc cgagcgccgg gcggcaccga cgtcctcgcg
    18361 atgatgctcg aagcacgcga cgagcaaggc aaggcgatga ccgacgtcga ggttcgcgac
    18421 gagctgatca cgctactgct cgccggtcac gagacgactg cgaccgcgct cgcgtggacg
    18481 ttcgatcaac tgctcgcgaa tccgcagacg ttcgacaaac tacaggcgga gctcgccgcc
    18541 ggaagcgaca gctatctcga ctgcgtgatc cgcgagacgc tgcgcgtccg accgatcgtg
    18601 ccgctgatcg gccgccacgt cgcgaaaccg ttccagctcg gcaactggat gattccgccg
    18661 ggacatcgca tcgcaccgtc gatctatctc agcggccaga accccgcggt ctatcccgag
    18721 ccgaagcgct tccagccgga gcgctggatc ggcgtcaaac cggatccgta cacgtggcta
    18781 cccttcggcg gcggcgtccg tcgctgcatc ggcatggcgt tcgcgcagtt cgagatgcgc
    18841 atcgtgctgc agaccgtggt ggcgcgtgcg cgcatgcacc tcgcggacgg tcccgcaaag
    18901 gtggtccgtc gcgggatcac gctcgcgccg tcgggcggca ctcgcgtcgt gctcgatgaa
    18961 cgcatcgcga cgcgtcctgc gccgcgggcg gtctaccagg cgtagcggtt gcccttccag
    19021 ttcaagaact cgcccgaatc agcgagcgtc gcgcgatcga tctcgcgcag gatgccgcgc
    19081 accgaatcct cgacgcgcgt cggcgcgttg gcgccaccca tgtcggtctg cacccagccg
    19141 ggattgatca cgtaggacg
//
LOCUS       DASUFX010000741         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1923786, whole genome shotgun sequence.
ACCESSION   DASUFX010000741 DASUFX010000000
VERSION     DASUFX010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923786"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1189
                     /locus_tag="VFV99_29975"
     CDS             35..1189
                     /locus_tag="VFV99_29975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5063658.1"
                     /translation="MADLYGEGNERAAQSIADIVAGVDADVLHLHAFTRAASVRAARA
                     AKRTGARVVFTYHTPTASCVRGTLLRDGQEPCDGALIAQRCAACLLQSRGVPLVAREV
                     LSRAPATIGSALVSAHVAGRWVTGLRARELTAMRHDAFRQLLDESDAIVAVCGWVRSL
                     LLRAGAPPDRVVLSRQGTPLTPSPVQPRRARDAELRLAYVGRLHPTKGVHIVLGALAA
                     CPELRLTLDVYGIAQGDEGARYRDELVAITASDSRVRFQAPLASDRVVETLASYDATV
                     VPSQWLETGPLTVLESFAAGTPVIGSNLGGIAELVSHDLDGWLVRHADVEAWTLALRR
                     VATDRTLLNRLRAGVHAPRSTTDVARDMVAVYESIVSRRASGSPATAGVA"
     gene            1189..>2072
                     /locus_tag="VFV99_29980"
     CDS             1189..>2072
                     /locus_tag="VFV99_29980"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5063659.1"
                     /translation="MRVLYVQYTNPGAYPPLVRGSSLLAESGAEVLMLGTRVRDTDVL
                     DVKPAKGIDVRLTQQAPEGWRLKTHYGRYAAWVAREAAAWKPDWIYASDLLSAPIALA
                     LAALTRARIVYHEHDAPSTDRPNWIIKRYLDARRRLLHNADLVVTPNAERSARLAALA
                     GGREVLTVWNCPRRPDRRPPMAQPRDVLRVIYRGSVNSERLPLTAIEAVARADLPVTL
                     DIAGYETAGSRGYLARLGDHAGRLGTGNRVRVLGTISEDELGVICENCDIGLALMPIA
                     SADENMRTMAGASNKAFE"
BASE COUNT          320 a          683 c          708 g          361 t
ORIGIN      
        1 ggtttgtcga ttcgcgcttg gcgagccgga gaacgtggcc gacctgtacg gagaagggaa
       61 tgagcgcgcg gcgcagtcga tcgccgacat tgtcgccgga gtggatgccg acgttctgca
      121 cctccacgct ttcacacgcg cagcgtccgt gcgtgccgcg cgcgccgcga agcgaacagg
      181 ggcgcgcgta gtcttcacct atcacacgcc gacggcgagc tgcgtacgcg gcacgctcct
      241 tcgtgacggc caggagccgt gtgacggagc gctgatcgcg cagcggtgtg ccgcatgtct
      301 gctgcagagt cgcggcgtac cgctcgtggc tcgcgaagtg ctctcgcgag cgccggccac
      361 gatcggctct gctctggtgt cggcgcatgt ggccggccgc tgggtgaccg ggctcagagc
      421 gcgcgagctg acagccatgc gacacgacgc atttcggcaa ctgctcgacg agagcgacgc
      481 gatcgttgcc gtgtgcgggt gggtgaggtc attgctgctg cgagccggtg cgccgcccga
      541 tcgcgtggtc ctttcgcggc aaggaacgcc gctgacacca tcgcccgtgc agccgcggag
      601 ggctcgcgat gcggagcttc ggctcgccta cgttggccga ctgcatccca cgaagggtgt
      661 ccacatcgtg ctgggtgcgc tcgcggcctg tccggagctg cgcctaacgc tcgacgtgta
      721 cggaatcgcg cagggcgacg agggtgctcg gtaccgagac gagctcgtcg caatcactgc
      781 gtccgactcg cgcgttcgat ttcaagcacc gctcgcgtcg gatcgagtcg tggagaccct
      841 ggcgtcgtac gatgcgaccg tcgtcccctc gcagtggctc gagaccgggc cgctgaccgt
      901 gctcgagtcg tttgccgccg ggacgcccgt gatcgggtcc aacctcggcg gcatcgccga
      961 gttggtctcg cacgatctcg atggctggct cgtgcgccac gccgacgtcg aggcgtggac
     1021 gctcgctttg cgccgcgttg cgaccgatcg gacgctgttg aatcgactgc gtgcgggcgt
     1081 tcacgctccg cgatccacga ccgacgtggc ccgagacatg gttgccgtgt acgagtcgat
     1141 cgtgtcgcga cgcgcgagtg ggtcgccggc gactgccgga gtggcgtgat gcgcgtgctc
     1201 tacgtgcaat acacgaatcc tggcgcatat ccaccgctcg tgcgcggctc gagcctgctc
     1261 gcggaatcgg gcgccgaggt tttgatgctt ggcacgcggg tgcgcgacac cgacgtgctc
     1321 gacgtgaagc ccgcgaaagg catcgacgtt cgtctcaccc agcaggcgcc cgaaggttgg
     1381 cgactcaaga cgcattatgg tcgctacgcc gcctgggtcg cgagagaagc ggccgcctgg
     1441 aagcccgatt ggatctacgc ttccgatctg ctatcggccc ccatagcgct cgcgctggcc
     1501 gcgctcacac gtgcccggat cgtctaccac gagcacgacg cgccctcgac cgaccggccg
     1561 aactggatca tcaaacgcta tctcgacgct cgtcgccgcc tgctccataa cgccgatctc
     1621 gtcgtgacgc ctaacgccga gcgatcggcg cgactggccg cgctcgccgg tggccgggag
     1681 gtgctgacgg tctggaactg cccgcggaga cctgatcgac gaccgccgat ggcacagccg
     1741 cgcgacgtcc ttcgcgtcat ctatcgtggg tccgtgaact ccgagcggct gccgctcacc
     1801 gccatcgaag ccgttgcacg agccgattta ccggtgacac tggacatcgc cggctacgaa
     1861 acagccgggt cgcgtgggta tctcgcgagg ctcggcgatc acgccggtcg tctggggacc
     1921 ggaaatcggg tccgcgtcct cggcacgatt tctgaggacg aactgggcgt gatctgcgag
     1981 aattgcgata tcgggctggc gctcatgcct atagcgtcag ccgacgagaa catgcgtacc
     2041 atggcgggcg cctcgaacaa agcgttcgag ta
//
LOCUS       DASUFX010000742         5809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_259257,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000742 DASUFX010000000
VERSION     DASUFX010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259257"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1276)
                     /locus_tag="VFV99_29985"
     CDS             complement(206..1276)
                     /locus_tag="VFV99_29985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063660.1"
                     /translation="MKKALVILACLAAPAIAAPKKKKAPPKKPTPAEKAPPPEAEPAV
                     APAPAPSPVSSENKPWAQGVPQDVQDKAKALYEEGNTLFAQQAHAPALEKYKQAIAMW
                     EHPLIRFNMAVTEIRLERILEAADDLEKALAYGDKPFKPELYQQALDYQALVKGRVGF
                     IEATCDDTATRLLLDGKPWTDCPKTVKIRVLAGEHAIVGEKSGYLTASTKVVVAGGKT
                     SQEKVHLVSLDSANVVKYRWRRWIPWTMTGMGVAVGLGGVGTWFLGKRQMDQFEADFS
                     TQCQNGCEPGLTAPEHRSLLAQKNGAELKGQIGVVMMGVGGAVAVTGIVLAIMNRGEK
                     VMPNVEVAPTAGGGTAKVSWGF"
     gene            complement(1276..5067)
                     /locus_tag="VFV99_29990"
     CDS             complement(1276..5067)
                     /locus_tag="VFV99_29990"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063661.1"
                     /translation="MGESKSSAGEDPPTTVGSAEDNDLDLALDRLMTPGSAPAAAPPV
                     PEHTMAPQPKLRPPAPPMASPRGSQSLQRPPSEPGKPKPIVPTPANAPRPKSVTAPSG
                     STTPAPRVPTPPAMAKVPDKANVPRVPTPPAMAKVQAAPPRPPPLRAQTPPPGVAASA
                     TATARKIEPAPVTPPTEPSAASSSGNMPAASSGQWTAENLSPPTAMPSAKLRPPAAPA
                     ASLASAVPITHSAVPVEVAPTQSASNAIPEPIAHKIQSSPHGAEDLNVPASKRRTAPL
                     PADPLASNGADRAPASGEFPAARPRTAKPSQATSLLPADQQFPRPEPRSGGSGSKPRR
                     EPRQPIEIGESTQIDRFGLIREIARGGMGQVFLARDTKLGRKVAIKFLLHDDPNFAQR
                     FLIEARATARCTHENIVAIYEVGEYQGLPYMVLEYLEGKTLSQVLEAKPGPRQFAELM
                     LSVFRALERAHEHGIVHRDLKPSNIFVTDRGQVKVLDFGVARIFDPAGDDAERIAANS
                     SKDKRWESTTPEGERHTYVTFSGGGTMVGTLPYMSPEQWGADTVDLQSDIWACGIMFW
                     RALTGVHPAGTMNADKLRVRLTDLDTPLPSLGARDPSLPAELVAIVDRCLQKHKSERY
                     QTATQAIQDLQAFLAPKAERISPDVCPYRGLASFGENDAKYFFGRSNEIRTAVAQLEA
                     WPLLAVIGPSGVGKSSFVHAGLLPAMRATGGTWHVRVLRPGRQPLQSLASALDETIDT
                     GVAPADVIDQLGDSPGLFGEYLRKAAIRKKHKVMLVVDQLEELFTLSDNDEVRKTFLA
                     ALLAAADDATAPVRVVLSMRADFLDRLAGHKQFLSELSRGLFFLSAPDLDNLRETLVR
                     PAELAGYTFEDSWIVEDMMQAATSKGALPLLQFAATRLWDARDRTKRKLTIAAYNAMG
                     GVGGAFARHADEVAAAVPSQSQKLLRAMMTRLVTAEGTRAVIDHTELLELSSDRQEVE
                     RILDQLVRARLVLMHTEGSEGTTVEIVHEVLISEWPTLARWLEDSQAMRGFMQELRQA
                     TKQWQSKGKRSDLVWRGAQAQDALATTKRHLLDLSSQERDFLDAVRKQLASSRRKRVL
                     GLSFVFVVLGVILAGGAFFTIQLAKANSEANDALVEAKKAKAQVQAQLDEVKAAQERK
                     DKAEAEAKQAAEIAAAKAAEAQKANQQVAQSQEDLAAANLALRKKVEEAEKANATAQE
                     LARVAQENAKKAATAKEVAEKATAEAKAEKARTQQLLAAEKERVKQLEAEKSKISTGG
                     L"
     gene            complement(5169..>5809)
                     /locus_tag="VFV99_29995"
     CDS             complement(5169..>5809)
                     /locus_tag="VFV99_29995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5063662.1"
                     /translation="AAAMIYPRVKEHIEFDELVALGNAGLAEAATRYDPDRGASFSTF
                     AWYRVQGAIVDGLRKSSNLPRRVWAKLVALRAASEYLEHRAERDAGAAQRGAKPPERE
                     DALASVKSALSAIRTMYVTSLESMQDQGFDAADPDAKTAGERIDVTRLSAKLRNALAA
                     LPEKERALVTKHYWEGKNLLEAGAELGISKSWASRLHAQAVDRLRVLVDAET"
BASE COUNT          847 a         1915 c         1950 g         1097 t
ORIGIN      
        1 gacgaggatg ttcctcatcc cgtagaagct gaagcgctcg cagccttcgt tcgcgatgat
       61 gaacggaatc tgcgacggga ttttcgcttt gggcttctcg ctcacgcgcc gcgcatacta
      121 ccggatcgtg ccctgcgcgc tatgtgaaca agcgctaacc ggcagcgtcg cgcggcttac
      181 gtagacaagc gcgtcagaag cgctgctaga agccccagct gactttcgcg gtgccgccac
      241 cggcggtcgg cgcgacctcg acgttcggca tcaccttctc gccgcggttc atgatcgcga
      301 gcacgatgcc ggtcacggcg accgcgccac cgacgcccat catcacgacg ccgatctggc
      361 ccttgagctc ggcgccattc ttctgcgcga ggagcgagcg gtgctccggc gcggtgaggc
      421 cgggctcgca gccgttctgg cactgcgtcg agaagtcggc ttcgaactga tccatctggc
      481 gcttgccgag gaaccacgtg ccgacgccac cgaggcccac cgcgacgccc atgcccgtca
      541 tcgtccacgg aatccagcgc cgccagcgat atttgacgac gttggccgag tcgagcgaga
      601 cgaggtgcac tttctcctgc gacgtcttgc cgccggcgac gacgaccttc gtcgacgcgg
      661 tgagatagcc gctcttctcg ccgacgatcg catgctcgcc ggcgaggacg cggatcttca
      721 cggtcttcgg gcagtcggtc cacggcttgc catcgagcaa gaggcgcgtc gcggtgtcgt
      781 cacaggttgc ttcgatgaag ccgacgcggc ctttgacgag cgcctggtag tcgagcgcct
      841 gctgatagag ctcgggtttg aacggcttgt cgccgtaggc gagcgctttc tcgagatcgt
      901 cggcggcttc gaggatgcgc tcgaggcgaa tctcggtgac ggccatgttg aagcgaatca
      961 gcgggtgctc ccacatcgcg atcgcttgct tgtacttttc gagcgcgggc gcatgcgcct
     1021 gctgcgcgaa tagcgtgttg ccttcctcgt agagcgcctt cgccttgtcc tgaacatcct
     1081 gcgggacgcc ctgcgcccac ggcttgttct cgctactgac cggcgacggc gcgggcgctg
     1141 gcgcgacggc aggctctgct tctggtggcg gcgccttctc cgcgggcgtt ggcttcttgg
     1201 gcggagcttt cttcttcttc ggcgcggcga tggccggagc ggcgagacac gcgaggatga
     1261 cgagcgcttt cttcatcaca gtcctcccgt cgagatcttc gacttctcgg cctcgagctg
     1321 tttcacgcgt tccttctcgg cagcgagcag ctgctgcgtg cgcgccttct cggccttcgc
     1381 ttcggcggtc gccttctcgg cgacctcctt cgcagtggcc gctttcttcg cgttctcttg
     1441 ggcgacgcgc gcgagctctt gcgcggtcgc attcgccttc tcggcttcct cgaccttctt
     1501 gcgaagtgcg aggttggcgg cagcgaggtc ttcctgcgac tgggcgacct gctggttcgc
     1561 cttctgcgct tccgcggcct tcgccgctgc gatctcggct gcctgcttcg cctccgcttc
     1621 ggccttgtcc ttgcgctcct gcgccgcctt cacctcgtcg agctgcgcct gcacctgcgc
     1681 tttggctttc ttggcttcga cgagcgcgtc gttcgcttcc gagttcgcct tggcgagctg
     1741 gatcgtgaag aacgcaccgc cggcgaggat cacgccgagt acgacgaaca cgaacgacag
     1801 gccgagcacg cgcttgcggc ggctgctcgc gagctgtttg cgcacggcat cgaggaagtc
     1861 gcgctcctgg ctcgacagat cgagcaggtg gcgcttcgtc gtcgcgagcg catcctgcgc
     1921 ctgcgcgccg cgccacacga ggtcgctgcg cttgcccttc gactgccact gtttcgtcgc
     1981 ttgccgcagc tcctgcatga agccgcgcat cgcctggcta tcttcgagcc agcgtgcgag
     2041 cgtcggccac tcactgatca gcacctcgtg gacgatctcg acggtcgtgc cttcggagcc
     2101 ctcggtgtgc atcagcacga ggcgcgcacg cacgagctga tcgaggatgc gctcgacctc
     2161 ttggcgatcg ctcgacagct cgagcagctc ggtgtgatcg atgaccgcgc gcgtgccctc
     2221 cgcggtcacg agtcgcgtca tcatcgcgcg cagaagcttc tggctttgcg acggcaccgc
     2281 ggccgcgact tcatcggcgt gacgcgcgaa cgcgccaccg acgccgccca tcgcgttgta
     2341 cgcggcgatc gtcagcttgc gcttggtgcg atcgcgcgca tcccagaggc gtgtcgctgc
     2401 gaactggagt agtggcagcg cgcccttgct ggtcgccgcc tgcatcatgt cctcgacgat
     2461 ccacgaatcc tcgaacgtgt agcccgcgag ctcggccgga cggacgagcg tctcgcgcag
     2521 gttgtcgagg tcgggcgccg acaggaagaa caagccgcgc gacagctcgg agaggaattg
     2581 cttgtgaccg gcgaggcgat cgaggaagtc agcgcgcatc gacagcacga cgcgcaccgg
     2641 cgcggtcgca tcgtcggcgg cggcgagcag cgctgcgagg aacgtcttgc gcacctcgtc
     2701 gttgtcggaa agcgtgaaca gctcctcgag ctgatcgacg acgagcatca ccttgtgctt
     2761 cttgcggatc gccgccttgc gcaaatactc gccgaacaga ccgggtgaat cgccgagctg
     2821 atcgatcacg tcggccggtg cgacgccggt gtcgatcgtc tcgtcgagcg cgctcgcgag
     2881 gctctgcaac ggctgacggc caggacgcag cacgcggaca tgccacgtgc cgccagtcgc
     2941 gcgcatcgcg ggcaacaggc cagcgtgcac gaacgacgat ttaccaacac ccgacggacc
     3001 gatgacggcg agcagcggcc acgcctcgag ctgcgcgacc gccgtacgga tctcgttcga
     3061 gcggccgaag aagtacttcg catcgttttc gccgaacgac gcgagcccgc gatacgggca
     3121 gacgtcgggg ctgatgcgct cggccttcgg cgcgaggaac gcctgcaggt cctggatcgc
     3181 ctgcgtcgcg gtctggtagc gctccgattt gtgcttctgc aggcagcgat cgacgatcgc
     3241 gacgagctcg gcgggcagcg atggatcacg cgcgccgagg ctcggcagcg gtgtatcgag
     3301 atcggtgagc cgaacgcgca gcttgtcggc gttcatcgtg ccggccggat gcacgccggt
     3361 cagcgcgcgc cagaacatga tgccgcacgc ccagatgtcc gattggagat cgacggtgtc
     3421 ggcgccccat tgctcgggcg acatatacgg cagcgtgccg accatcgtgc cgccgccgga
     3481 gaacgtgacg tacgtgtgac gctcgccctc gggcgtggtc gattcccagc gcttgtcctt
     3541 gctcgaattc gcggcgattc gctcggcgtc gtcgcccgcg ggatcgaaga tgcgtgcgac
     3601 gccgaagtcg agcaccttca cctggccgcg atcggtgacg aagatgttgc ttggcttcag
     3661 atcgcggtga acgatgccgt gctcgtgtgc gcgctcgagc gcgcggaaca ccgacagcat
     3721 cagctcggcg aactggcgcg ggcccggctt cgcctcgagc acctgcgaga gcgtcttgcc
     3781 ctcgagatac tcgagcacca tgtacggcag gccttggtac tcgccgacct cgtagatcgc
     3841 gacgatgttc tcgtgcgtgc aacgcgccgt cgcgcgcgcc tcgatgagaa agcgctgcgc
     3901 gaagttcgga tcgtcgtgga gcaggaactt gatcgcgacc ttgcggccga gcttcgtgtc
     3961 gcgggcgagg aagacctggc ccatgccacc acgcgcgatt tcgcggatca ggccgaagcg
     4021 atcgatctgc gtgctctcac cgatttcgat cggctgtcgt ggctcgcgac gcggtttcga
     4081 cccagagcca ccactacgcg gctcgggcct ggggaactgt tgatccgcgg gcaacagcga
     4141 cgtcgcctgg ctcggcttcg ccgtgcgcgg gcgcgcggcg gggaattcgc ccgacgctgg
     4201 cgcgcgatcg gcgccgttcg acgcgagcgg atccgcgggt agcggcgctg tccgccgctt
     4261 gctcgccggg acgttcaggt cctcggctcc atgcggcgac gactggatct tgtgcgcgat
     4321 cggttccgga atcgcgttgc tcgcgctctg tgtcggcgcg acttcgaccg gaactgccga
     4381 gtgcgtgatc ggcaccgcgg aagcgagtga cgccgctggc gcagcaggcg gacgaagctt
     4441 cgcggagggc attgccgtcg gcggcgagag gttctctgcc gtccactggc cactcgatgc
     4501 cgcgggcatg ttgcccgacg acgatgctgc cgatggttcg gtcggcggcg ttacgggtgc
     4561 gggttcgatc ttgcgtgcag ttgcagtcgc cgaggcagcg acgcccggtg gtggcgtctg
     4621 tgcgcgaagt ggtggcggac gcggtggcgc cgcttgaact ttcgccatcg caggcggcgt
     4681 cggcacgcgc ggcacgttcg ccttgtcggg aacctttgcc atcgcaggtg gcgtcggcac
     4741 gcgcggtgcg ggcgtcgtgc tgcctgatgg cgcggtcacc gacttgggac gcggtgcatt
     4801 cgcgggcgtc ggcacgatcg gtttcggctt gcccggttcc gatggtggac gttgaagtga
     4861 ttgcgatcca cgtggtgatg ccatcggcgg tgcaggcggc cgcaacttcg gctgcggcgc
     4921 catcgtgtgt tccggaactg gaggcgccgc cgcgggcgcg gaacctggcg tcatcaggcg
     4981 atcgagcgcc aggtccagat cgttgtcttc ggcagacccg accgtagtcg gcggatcctc
     5041 tcccgctgag gacttagact ctcccacgcg tagccttcat tgtagatcga gcttcgcggt
     5101 gttagcgatg tgggaggcga caccacacac gcgagatcag ctgcatcgca gcttcgtggc
     5161 gtcgacggtc aagtctcggc gtcgacgagc acgcgcaacc tgtcgacagc ttgtgcgtgc
     5221 aaccggctcg cccacgactt ggagatgccc agctcggcac ccgcttcgag gaggtttttg
     5281 ccctcccagt agtgtttggt gacgagcgca cgctcttttt cgggtagcgc agcgagtgcg
     5341 tttcggagct ttgccgagag gcgggtgacg tcgatgcgct ctcccgcagt ctttgcgtcg
     5401 ggatcggcgg cgtcgaatcc ttggtcctgc atcgactcga gcgacgtcac gtacatcgtc
     5461 cggatcgccg acagcgcgct cttcacggac gcgagtgcat cttcccgttc gggaggcttc
     5521 gcgccacgct gcgccgcgcc cgcatcacgt tcagcgcgat gctccaagta ctcgctcgct
     5581 gcacgcagcg cgacgagctt ggcccagacg cggcgaggca ggttcgacga cttgcgcaag
     5641 ccatcgacga tcgcgccttg cacgcgatac cacgcgaacg tcgaaaacga tgcgccgcga
     5701 tccggatcgt agcgcgtggc agcttcagca agcccggcgt tcccgagcgc gacgagctcg
     5761 tcgaactcga tgtgctcttt gacgcgcgga tagatcatcg ccgcggcct
//
LOCUS       DASUFX010000743         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1924279, whole genome shotgun sequence.
ACCESSION   DASUFX010000743 DASUFX010000000
VERSION     DASUFX010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924279"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFV99_30000"
     CDS             complement(<1..818)
                     /locus_tag="VFV99_30000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063663.1"
                     /translation="MSPLLVPGETCAALVDVERSGVLVDGKDFYRAFYDACCKAERTI
                     LMAGWQFSCNTRLVRGDEVECCEYPHTLLDLLTTLCDERPDLHVYMLPWDSSPVFAFE
                     REPMQRLRLRLRGHDRIHWKMDHMHPAGASHHQKLFVVDRAIAFLGGMDVCKTRWDDR
                     SHEARNRTRDRRRRPYHDVSAYVTGEAVDVLRRWFTQRWEIATHSRLELPEQPRTMID
                     IRSSFDIDVPRVGFARTWPAMEKSDQPELRELLRLHQRAIGQAKHTIYIENQYFS"
     gene            complement(888..1592)
                     /locus_tag="VFV99_30005"
     CDS             complement(888..1592)
                     /locus_tag="VFV99_30005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5063664.1"
                     /translation="MRRLRVMTYNIHRGRGVDMKHDLGRIAAVIESYEPDVIALQEVD
                     VGCKRSGGIDQAHELASRLGMDMAFAPNLEWTNGERYGIATLTKLPIQSSRQLKLPQQ
                     YRSEPRSSLITVLEWREGHVVEMINTHLSILFKERPGQVAAIAAAMANEALIVAGDFN
                     MTPWSPAYRALRRDSFLHSATRFARTWPAPAPFMPLDHILYRGQVEVVRAEAWSGGPA
                     RTASDHLPVVVELQAA"
     gene            complement(1670..2158)
                     /locus_tag="VFV99_30010"
     CDS             complement(1670..2158)
                     /locus_tag="VFV99_30010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063665.1"
                     /translation="MRKIFAYSLLSAAIAVSGACKKSAEDKAEHNFEKARENVKEQTN
                     DVNKAEKDLRDEQKDVAKEQRDLAGQQRELGQAQQQLSVAREQYKASLTAELQQADAR
                     INELEAKGDEKSREKATRLRAKRSELAARMDKIGDQTDANWEEFKKDTDKKANDLKDD
                     LD"
     gene            2366..4477
                     /locus_tag="VFV99_30015"
     CDS             2366..4477
                     /locus_tag="VFV99_30015"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063666.1"
                     /translation="MIGQVIGNYRIVSELGRGGMGMVYRAEHVQLGRPAALKMLLPAL
                     SSDPGIVQRFFNEARAASAIDHPGIVEVYDFGTHTDGSAYIVMPLLKGESLESRLRNG
                     QVAPLEGASWVVQVLSALSAAHARGIVHRDLKPDNIFLVANELMPGGIQVKLLDFGIA
                     KLADDKAAGVKTQTGMMIGTPAYMSPEQCMGRSDLDHRTDIYSIGCILFHVVCGRPPF
                     LSEHGTGMIIAAQMRDAPPDPRSLNPNIPPALAAVILKCLEKDPAARYQTANDVKNAL
                     IGAGAQGTVSRAPVTSQDSYAATLAPSQSPSKPPSHASMSYDATVAPGPTTTRSGSAA
                     QMVAATQPPAKSSRGAYFAVGGVLLALAGIGVGVAMSRGDDDKSDKAPPVAAPEAAKP
                     AGTNPTTAKAEPVIPEAEKPAEKKPADTKPAELPACDEGMTRTIDTGDHCCWTGQAWS
                     NTKNKCIGAPTCPPSMRAKGEACIAAPTPTPTVPGSTAPASTAPAQIATAGHPTFKLD
                     AKTYAPGDPITIKFASALSSKPNNRAWVTWIDAGKPQTAYATWEYVADGATTAQLAAP
                     KQPGSYEMRLHTNYPTQSYNVVHTVPFTVDEAAASTKPATTKYRFHVNKKTATAGESI
                     ELTFGQPMIAAKGERFWVTIVKPGAADDSYGKYEYVPDNAHKMMFEMPGEPGDYELRL
                     HANYPTKSTNVVHRVKIHVDG"
     gene            4492..4893
                     /locus_tag="VFV99_30020"
     CDS             4492..4893
                     /locus_tag="VFV99_30020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5063667.1"
                     /translation="MQTVTTFVPRDADTCWRVFTDIPKLTQWVPGLRHAEIISKARGL
                     PSEVHFQFASSLAYTLVYMYDRTKREIHFEPKLGRREGVSGWARFEPFEDGTRFTYAL
                     EHGDGRGAAERELGDLQRLVDAFSTWLTTTT"
     gene            complement(4890..>5240)
                     /locus_tag="VFV99_30025"
     CDS             complement(4890..>5240)
                     /locus_tag="VFV99_30025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063668.1"
                     /translation="TACVAAMTTSRALGCPRHLQCVVQPDESAPEIARAHKAVAIPDV
                     RSVHVAEDRLTLADAPRKAEPDAMPWIWRVLREQIYTRLPRHDDERFTLVLSPVVVTS
                     PSDTIPGVGIAGNF"
BASE COUNT          886 a         1746 c         1724 g          884 t
ORIGIN      
        1 gagaagtact ggttctcgat gtagatggtg tgcttcgcct ggccgatcgc gcgctggtgg
       61 aggcgcaaga gctcgcggag ctccggctgg tcgctcttct ccatggccgg ccacgtgcgc
      121 gcgaagccga cgcgcgggac atcgatgtcg aacgacgagc ggatgtcgat catcgtgcgc
      181 ggctgctcgg gcagctcgag ccggctgtgc gtcgcgatct cccagcgctg ggtgaaccag
      241 cggcgcagga cgtcgaccgc ctcgccggtc acgtaggcgc tgacgtcgtg gtaggggcgg
      301 cggcgccggt cgcgcgttct gttacgcgcc tcgtgcgaac gatcgtccca gcgcgttttg
      361 cagacatcca tgccaccgag gaacgcgatc gcgcgatcga cgacaaacag cttctggtga
      421 tggctcgcac cggccgggtg catgtgatcc atcttccagt gaatgcgatc gtggccgcgc
      481 aagcggagcc gcagtcgctg catcggctcg cgctcgaacg cgaagaccgg cgagctgtcc
      541 cacggcagca tgtagacgtg gaggtcggga cgttcgtcgc acagcgtcgt gagcaggtcg
      601 agcagcgtgt gcggatactc gcagcactcc acctcgtcgc cgcgcacgag cctcgtgttg
      661 caggagaact gccagccggc catcaggatc gtgcgctccg ctttgcaaca cgcgtcgtaa
      721 aacgcgcggt agaaatcctt gccgtcgacg aggacccctg accgctcgac atcgacgagg
      781 gccgcgcagg tctcgccggg gacgagcagg gggctcatac ccgtacgtcg agcgaatcgc
      841 gtgccatgca aagcgcgccg cgttgtcgca acgcgcgtca gcgcgcgtca ggcagcttgc
      901 agctcgacga cgacgggcag gtgatcagaa gcagtacgag ccggcccgcc gctccaggct
      961 tccgcacgta cgacctcgac ttgaccgcgg tacaggatgt gatcgagcgg catgaacggc
     1021 gcgggtgctg gccacgtgcg ggcaaaccgc gtcgccgagt gcaggaacga atcgcgacga
     1081 agcgcgcgat acgcaggcga ccacggcgtc atgttgaagt cgccggcgac gatcaacgcc
     1141 tcgttcgcca tcgcagctgc gatcgctgcg acctgaccgg gccgttcctt gaacaggatc
     1201 gacaggtgtg tgttgatcat ctcgacgacg tggccctctc gccactcgag aacggtgatc
     1261 agcgacgagc gcggctcgct gcgatactgc tgcggcagct tgagctgacg cgacgattgg
     1321 atcggcagct tcgtcagcgt cgcgattccg tagcgctcgc cgttcgtcca ctcgaggttc
     1381 ggtgcgaacg ccatgtccat cccgagccgg ctcgcgagct cgtgcgcctg gtcgatgccg
     1441 ccactgcgct tgcagccgac gtcgacctcc tggagcgcga tgacgtccgg ctcgtatgac
     1501 tcgatcaccg ccgcgatgcg gccgagatcg tgcttcatgt cgacaccgcg tccgcgatgg
     1561 atgttgtagg tcatcacgcg caagcgccgc atctgcgcca acgctagcgc cgaaacgacg
     1621 accgagcact cgggggaatt ccgagtgctc ggtgtgttgc gagtgagaac tagtcgaggt
     1681 cgtccttcag atcgttggcc ttcttgtccg tgtccttctt gaactcttcc cagttggcgt
     1741 cggtctggtc gccgatcttg tccatgcgcg cagcgagctc gctacgcttc gcgcggagcc
     1801 gcgtcgcctt ctcacgcgac ttctcgtcgc ccttcgcctc gagctcgttg atgcgcgcgt
     1861 cggcttgctg cagctcggcc gtcagcgagg ccttgtactg ctcgcgcgcc accgacagtt
     1921 gctgttgcgc ctggccgagc tcgcgctgct gaccggcgag gtcgcgttgc tccttcgcga
     1981 cgtctttctg ctcgtcgcgg aggtccttct ccgccttgtt gacgtcgttc gtctgttcct
     2041 tgacgttctc gcgagccttt tcgaaattgt gctcggcttt atcttctgcg ctcttcttgc
     2101 aggcgccgct gactgcgatc gctgcagaga ggagcgagta cgcaaagatc ttacgcatcg
     2161 gaatctccct tcgtgttgca gctttgttgc tgcaacacag gtgcccgcag atcgtggcgg
     2221 aattgcgtcc gcgaaccggt gcgctttggt gcgaccacgc gcgcagccgt gcgcgacctg
     2281 tcagcagctg gttacgattg ccggttctcg tcgacgaacc cactggatgg gcatcgcgcc
     2341 gccgcgcttg ctatgctcat gcggcttgat cgggcaagtc atcggtaact accgaatcgt
     2401 ctccgagctc ggccggggcg ggatgggcat ggtgtatcgc gccgagcacg tgcagctggg
     2461 acgaccggcc gcgctcaaga tgttgttgcc ggcgctgtcg agcgatccgg gcatcgtgca
     2521 gcgcttcttc aacgaagcgc gcgcggcctc ggcgatcgat catcccggca tcgtcgaggt
     2581 ctacgacttc ggtacgcaca ccgacggcag cgcgtacatc gtgatgccac tgctcaaggg
     2641 cgagagtctc gagagccggc tccgcaacgg gcaggtcgca ccgctcgaag gcgcgtcgtg
     2701 ggtcgtccag gtgctgtccg cgctgtcagc cgcgcacgcg cgcgggatcg tccatcgcga
     2761 tctcaaaccc gacaacatct tcctcgtcgc gaacgagctg atgcccggcg gcattcaggt
     2821 caagctgctc gacttcggca tcgcgaagct cgccgacgac aaggccgcgg gcgtcaagac
     2881 gcagacgggc atgatgatcg gcacgccggc gtacatgagc cccgagcaat gcatggggcg
     2941 ctctgatctc gatcaccgca ccgacatcta ttcgatcggc tgcatcctgt ttcacgtcgt
     3001 gtgcggacgt ccgccgttcc tcagcgagca cggcaccggc atgatcatcg ccgcgcagat
     3061 gcgcgatgcg ccgcccgatc cgcgctcgct gaatcccaac attccgccgg cgctcgccgc
     3121 ggtgattctg aagtgcctcg aaaaggatcc ggcggcgcgc taccagaccg cgaatgacgt
     3181 caagaacgcg ctgatcggcg ccggtgcgca aggcacggtc tcgcgtgcgc ccgtcacctc
     3241 gcaggattcc tacgccgcga cgctcgcgcc atcgcaatcg ccgtccaaac cgccgtctca
     3301 cgcgtcgatg tcatacgacg cgacggttgc accggggccg acgacgacac gcagcggatc
     3361 ggccgcgcag atggtcgctg cgacgcagcc gcccgccaag tcgtcgcgtg gcgcctactt
     3421 cgcggtcggt ggcgtgttgc tcgcgcttgc cggcatcggt gtcggcgtcg cgatgagccg
     3481 cggcgacgac gacaagtccg acaaggcgcc gccggttgcc gcgcccgagg cggcgaagcc
     3541 cgcaggcacg aatccgacga cggcgaaggc cgagccggtg atccccgaag ccgagaagcc
     3601 cgccgagaag aaaccggccg acacgaagcc cgcggagctg ccggcgtgtg acgagggcat
     3661 gacgcggacg atcgacaccg gcgatcactg ttgctggacc ggccaggcgt ggtcgaacac
     3721 gaagaacaag tgcatcggcg caccgacgtg ccctccgagc atgcgcgcga aaggtgaggc
     3781 ctgcatcgca gcgcccacgc ccacacccac cgtgccgggt tcgaccgcgc ctgcgtcgac
     3841 cgcgcccgcg cagatcgcga cggccggcca cccgacgttc aagctcgacg cgaagaccta
     3901 cgcacctggc gatccgatca cgatcaagtt cgcgagcgcg ctgagcagca agccgaacaa
     3961 ccgcgcgtgg gtcacgtgga tcgacgcggg caagccgcag actgcctacg cgacgtggga
     4021 gtacgtcgcc gatggcgcga cgaccgcgca gcttgccgcg ccgaagcaac cgggcagcta
     4081 cgagatgcgc ctgcacacga actacccgac gcagagctac aacgtcgtgc acacggtgcc
     4141 gttcaccgtg gacgaagcgg cggcatcgac aaagccggcg accacgaagt accgcttcca
     4201 cgtcaacaag aagaccgcga ctgccggcga gagcatcgag ctcacgttcg ggcagccgat
     4261 gatcgctgcg aagggcgagc ggttctgggt cacgatcgtc aagcccggcg ccgccgacga
     4321 ctcgtacggc aagtacgagt acgtcccgga caatgcccac aagatgatgt tcgagatgcc
     4381 gggcgagccg ggcgactacg agctacgcct gcatgcgaac tacccgacga agtcgaccaa
     4441 cgtcgtgcac cgggtcaaga tccacgtcga cggttgagtt actcttcgcc aatgcagacg
     4501 gtgaccacgt tcgtgccgcg cgatgcggac acttgctggc gcgtcttcac ggacatcccg
     4561 aagctcacgc agtgggtgcc gggccttcgc cacgccgaga tcatttccaa ggcgcgtggc
     4621 ctgccgtccg aggttcactt ccagtttgca agctcgctcg cgtacacgct cgtctacatg
     4681 tacgaccgca cgaagcgcga gatccacttc gagccgaagc tcggccgtcg tgaaggcgtc
     4741 agcggctggg cgcgcttcga accgttcgaa gacggcacgc gcttcacgta cgcgctcgag
     4801 cacggtgacg gccgcggcgc cgcggaacgc gagctcggcg atctgcagcg cctcgtcgac
     4861 gcattctcga cgtggctgac gacgacgacc tagaagttgc cggcgatacc cacgcccggg
     4921 atcgtgtccg acgggctcgt cacgactacc ggcgagagca cgagtgtgaa gcgctcgtcg
     4981 tcgtggcgtg gtagccgcgt gtagatctgc tcgcgcagca cgcgccagat ccacggcatc
     5041 gcgtcgggct ccgccttgcg cggcgcgtcc gcgagcgtga ggcgatcctc tgcgacgtgg
     5101 acgctgcgca cgtcggggat cgcgacggct ttgtgggcgc gcgcgatctc gggcgcgctt
     5161 tcgtcgggtt gcacgacgca ctgcagatga cgcgggcagc cgagcgcgcg acttgtcgtc
     5221 atcgcggcaa cacaggcggt
//
LOCUS       DASUFX010000744         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1369717, whole genome shotgun sequence.
ACCESSION   DASUFX010000744 DASUFX010000000
VERSION     DASUFX010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369717"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFV99_30030"
     CDS             <1..678
                     /locus_tag="VFV99_30030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5063669.1"
                     /translation="RVDVSNAEEVDAAAKRAREAFGVISVIVNNAGVGGGGGPMWKLG
                     VNDWKWALDVNLWGVINGIRALLGPLIESGEEGHVVNTASIAGLTSTPFMGPYTATKH
                     AVVSLSECLAKELELTKANVGVSVLCPGFVKTRIHQSHRNRPGEQQKVDDNPLAQKFA
                     AVLQQLVESGQSPEKIADEVVRAIKEPRFYILTHQEMKPAVEHRMRQILDEAQPGIDP
                     MFKALFG"
     gene            779..1144
                     /locus_tag="VFV99_30035"
     CDS             779..1144
                     /locus_tag="VFV99_30035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063670.1"
                     /translation="MIELIRGAYHEGGWGMYPTTFLGLVLIAAAIRYLRNPERRRLHL
                     ARHLNVLVGLSGTLGFFTGVIKTFTNLPSDQLYIAFIGVGESLHNVTLALCMMIVARI
                     IVAFGAAKDTDSASDLVAP"
     gene            complement(1155..2789)
                     /locus_tag="VFV99_30040"
     CDS             complement(1155..2789)
                     /locus_tag="VFV99_30040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063671.1"
                     /translation="MRSSVCVSILLFASVSHAEPTRNIELSVFDPTPTTVGTNFQVQS
                     ADVGHNGDLVISSWLSYANNPLVLGTVQNDDPVVRHRTMVELGGAYAFGDRFEAGARM
                     PFYLQSGEPVVKQMPGEPATFGVPPASGGVLGDLTVHGRARLWRNEQWLVGGGLVVKV
                     PTATDGEFAGTDMPSARALMLLTFVSSTQLAIHVNAGAVVRKKVEFANILQGSGATWG
                     AAMEYRFARALFFSAEVFGEVIPGGKVDEMNAESLAATIEGLAGLRFQASDQVSLGIA
                     AGRGVTNGVGAPAFRGVFTLAFTPSGRPLPPLHKPVVEAPIDPNTNDADFDKIVDAQD
                     KCPQEREDKDGFQDEDGCPDPDNDNDGVLDEKDKCQGQVEDKDGFQDDDGCPDDDNDN
                     DGVPDAQDKCPSEPEKINGVDDDDGCPDKGESSVISTPDRLELLESVIFKDAAVDKTS
                     FSVLQQLATTLRARGDIIRLRVGVHVQPTKNPAKDAALSQKRANALRDWLEAHGIDHE
                     RIDPKGFGGTKPLVAPTQKGAAAINDRVELIILERK"
     gene            complement(2782..>3250)
                     /locus_tag="VFV99_30045"
     CDS             complement(2782..>3250)
                     /locus_tag="VFV99_30045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063672.1"
                     /translation="GNGKDRNVAMGPSLDFGNTGAGVPVSYTALKVPDEWLTVINQDD
                     ADFKIREIKFDKPEFKVQTLSGDDVSNMDLPVGSTKQFEVIFLPPEVGEYTANMSIYL
                     DQDPTAQRTIEVHGRALFVDAHGGGGFGCSTGRGTGWGVLLALGLLRRKRRRA"
BASE COUNT          542 a         1080 c         1029 g          599 t
ORIGIN      
        1 cgtgtcgatg tgtcgaacgc ggaagaagtc gatgccgccg cgaagcgtgc ccgcgaggcg
       61 ttcggtgtga tctcggtcat cgtcaacaac gccggcgtcg gtggcggtgg cggcccgatg
      121 tggaagctcg gcgtgaacga ctggaagtgg gcgctcgacg tcaacctgtg gggcgtgatc
      181 aacggcattc gcgcgctgct ggggccgctc atcgaaagcg gcgaagaagg tcacgtcgtc
      241 aacacggcgt cgatcgctgg cctgacctcg accccattca tgggtcccta cacggcgacc
      301 aagcacgcgg tcgtctcgct ctccgagtgt ctcgcgaagg agctggagct gacgaaagcg
      361 aacgtcggcg tctccgtgct gtgtcccggg ttcgtgaaga cgcgcatcca ccagtcgcat
      421 cgcaatcgcc ccggcgagca gcagaaggtc gatgacaatc cgctcgcgca gaagttcgcg
      481 gcggtgctgc agcagctcgt cgagtcgggg cagtcgcccg agaagatcgc cgacgaagtg
      541 gttcgcgcga tcaaagagcc gcgcttctac atcctcacac atcaagagat gaagcccgcg
      601 gtcgagcacc gcatgcgaca gatcctcgac gaggcgcagc ccggaatcga tcctatgttc
      661 aaggcgctgt tcggctgatc ggtgcgacac cgcaccggcg tcgcagtggt gtcgcgcgca
      721 agtagttgca actgttagct gctgactctg tccagctcac acaaccaaag gagctaccgt
      781 gatcgaactt attcgtggcg catatcatga gggcggctgg ggcatgtatc cgacgacgtt
      841 cctcggcctc gtgctcattg ccgcagcaat ccgctacctg cggaacccgg agcgtcgccg
      901 gcttcacctc gcccgccatc taaacgtcct cgtcggactg tcggggacgc tcggattttt
      961 cacgggcgtg atcaagacgt tcacgaatct gccgagcgat cagctctaca tcgcgttcat
     1021 cggcgtcggc gagtcgctgc acaacgtcac gcttgcgctc tgcatgatga tcgtcgcacg
     1081 catcatcgtc gcgttcggcg ccgcgaagga caccgactcg gcaagcgatc tcgtcgcgcc
     1141 gtaggactac gcgttcattt gcgctcgagg atgatcagct cgacgcggtc gttgatcgcc
     1201 gccgcgccct tttgggtcgg cgcgaccaac ggcttggtgc cgccgaagcc cttcggatcg
     1261 atgcgctcgt gatcgatccc gtgcgcctcg agccagtcgc gcagcgcgtt cgcgcgcttc
     1321 tgcgatagcg cggcgtcctt cgctggattc ttcgtcggct gaacgtgcac gccgacgcgg
     1381 aggcgaatga tgtcgccgcg cgcgcgcagc gtcgtcgcga gttgctggag cacgctgaag
     1441 ctcgtcttgt cgaccgccgc gtccttgaag atcaccgact cgagcagctc gaggcgatcc
     1501 ggcgtcgaga tcacgctcga ctcgcccttg tcgggacagc catcgtcgtc gtcgacgccg
     1561 ttgatctttt ccggctcgct cgggcacttg tcttgtgcgt ccggcacgcc gtcgttgtcg
     1621 ttgtcgtcgt ctgggcagcc gtcgtcgtcc tggaagccgt ccttgtcctc gacctggccc
     1681 tggcacttgt ccttctcgtc gaggactccg tcgttgtcgt tatcgggatc gggacagccg
     1741 tcctcgtcct ggaagccgtc cttgtcctcg cgctcctgcg gacacttgtc ttgcgcgtcg
     1801 acgatcttgt cgaagtcggc gtcgttcgtg ttcggatcga tgggcgcctc gaccacaggc
     1861 ttgtgcagcg gcggcagcgg acgcccgctc ggcgtgaacg cgagcgtgaa cacgccgcgg
     1921 aacgcaggcg cgccgacgcc attggtcacg ccgcgccctg ccgcgatacc gaggctcact
     1981 tgatccgagg cctgaaagcg caatccggcg aggccctcga tcgttgccgc caacgattcg
     2041 gcgttcatct cgtcgacctt gccgcccggg atgacctcgc cgaacacctc ggccgagaag
     2101 aacagcgcac gcgcgaaccg gtactccatg gccgcgcccc aggtcgcgcc gcttccttgc
     2161 aggatgttcg cgaactcgac cttcttgcgc acgacggcgc cggcgttgac gtgaatcgcc
     2221 agctgcgtcg acgacacgaa cgtcagcagc atcagcgcgc gcgccgacgg catgtcggtg
     2281 ccggcgaact caccatcggt tgcggtcgga accttgacga cgagcccgcc accaaccagc
     2341 cactgctcgt tgcgccaaag ccgcgcacgc ccgtgcaccg tcaaatcgcc gagtacgccg
     2401 ccactcgccg gcggtacgcc gaacgttgcg ggctcgccgg gcatttgctt cacgaccggt
     2461 tcaccgctct gcagatagaa cggcatccgc gcgccggcct cgaagcggtc gccaaacgcg
     2521 tacgcgccgc cgagctcgac catcgtgcga tggcgaacca ccggatcgtc gttctgcacg
     2581 gtgccgagca cgagcgggtt attcgcgtac gacagccagc tcgagatcac gagatcgccg
     2641 ttatggccga cgtcggcact ctgaacctgg aagttcgtgc cgacggtcgt tggcgtcgga
     2701 tcgaacacgg agagctcgat gttgcgcgtc ggctccgcgt ggctgacaga agcgaacaga
     2761 agaatcgaga cgcagacgga gctacgcacg ccgccgcttt ctgcgcagca gaccgagcgc
     2821 gaggaggacg ccccagcccg tgccgcggcc ggtcgagcag ccgaagccgc caccgccgtg
     2881 cgcgtcgacg aatagcgcgc ggccatgcac ctcgatggtg cgctgcgcgg tcggatcttg
     2941 atcgaggtag atcgacatgt tcgccgtgta ctcgccgacc tcaggcggca agaagatcac
     3001 ttcgaattgc ttcgtgctgc cgaccggcag gtccatgttg ctgacgtcat cgccactgag
     3061 cgtctgcacc ttgaactcgg gcttatcgaa cttgatctcg cgaatcttga agtcggcgtc
     3121 gtcctgattg atgacggtca gccactcgtc cggcaccttc agcgcggtat acgaaaccgg
     3181 cacgccggcg ccggtgttgc cgaagtcgag cgacggtccc atcgcgacgt tgcggtcctt
     3241 gccgttgccc
//
LOCUS       DASUFX010000745        16177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2065033, whole genome shotgun sequence.
ACCESSION   DASUFX010000745 DASUFX010000000
VERSION     DASUFX010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065033"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="mutS"
                     /locus_tag="VFV99_30050"
     CDS             <1..1307
                     /gene="mutS"
                     /locus_tag="VFV99_30050"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5063673.1"
                     /translation="PYVLKDGGVIREGYDATVDECRRLADGGKDEILAIEAREQKDSG
                     IANLKVRYNRVFGYYIEITKTHLAKVPAHYVRKQTIATGERYVTPELAELEKKVLTAE
                     ETLASREAELFKTEVMAVAEVAAKISAAGAALAILDACGSLADVAAKRGYCRPVVDDN
                     LVIDLVDARHPVIEALLPPGSFVPNDCRLDPAAEQLVLITGPNMAGKSTYMRQVAQIV
                     LLAQIGSFVPAKAATIGVCDRVFTRVGAADNLSRGDSTFMVEMRETSSILAKATKRSL
                     VVLDEVGRGTSTFDGVSIAWAVAEHLHDAIGARTLFATHYHELVALADSRPRVRNVSV
                     AVREHKNEIVFLRRVIAGGANKSYGIDVARLAGLPRNVVNRAREIMAKLEGGNQLGST
                     PQLSLLPTAPPSPALARIAAIDIDRMTPLEALQLLAELKSLL"
     gene            1475..3751
                     /locus_tag="VFV99_30055"
     CDS             1475..3751
                     /locus_tag="VFV99_30055"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063674.1"
                     /translation="MRNIKSIAIGAALFTSVFGGGCGNSDDSGPLGNVDSLVILQRPK
                     RNDMGDIFQYTSYVPTARLVKLSPPTADGTLTTLCCDKAGAEFADIDISSYDISFDAK
                     EIVFSGKLKGSDRYGLFRLTLESGAVTQIATDPGRDYVNPIYLPGDKVMFQTNLAVNN
                     MAMDPVANTPIDANPAQHVDEYERGTTLQMGTVGLDGTGEVLGPRNLSHRTFPTLTSD
                     GRVMFTQWDHLGPVNAGHLMFMNTDMTELREAFGKEGTGASNSTLKAREISPGRFVGI
                     ATSRDRTIQSGALIDIRLGMPSTKNGVLRADTAMSEANASYVMLTPDVPTDREPSANT
                     IGRYYDAFPLNAGEHPDLLVSWADGPVESSVLGSAGISANFGVYLYDTKTLQRHPILD
                     DPEMWDIFARPLMPRSAPAVTSSSIDNSLGGQAMVGAMNVYESSLTTFQPGSIYGVRI
                     MEGFSSEEGFPEDFGTTMFEGQANLGVARVASDGSWLATVPANVPLHLQAIDVFGMSR
                     ENEPVWFSARPGESRVCGGCHENRATTTVINPGITQAAAIGPTDAKGLVSRARRISTD
                     AELSSPTTAPDRLVGMAWNKAIQPVFDAKCVSCHDGTPSAANPTYQIVDPTTGTMVAQ
                     FTFNLTATPVSIDYGMGEVQEWPASYLSMAGPDMEAIEMGNLMVTGTLPCKTVGGQPT
                     LQCMEPQNSRDSYVIRLVNPTQLFPTPSTTRAFNTSPHSSVGYTELTSAEFYKLILAA
                     DMGVNYYARENNPMLNRY"
     gene            3779..4612
                     /locus_tag="VFV99_30060"
     CDS             3779..4612
                     /locus_tag="VFV99_30060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5063675.1"
                     /translation="MNISKKVAAVAFVLLCGAAPAFAGKGGSNAKIVAAVQSGSVDAI
                     TAEVERAEGLMCPECVGTVLNLLEDNRFAVREVAAWWFAKRPGAKDQLATQFKAELST
                     SGSIAVRNAADFLGRVRQFDALPQLRVAIKRTDISAEAKLAIVRAVGYMAHPDGNSVL
                     LTAMNDGDASVRAAAVTAWREILGQLDVKPLVSKLADSDASVRAAAAMVVGVYGEPSA
                     RTQLEQLVTTDTDSFVRRNAAWALGRIGDNASAQALQTATNDKSGIVRGYASAALAEL
                     K"
     gene            4687..7680
                     /locus_tag="VFV99_30065"
     CDS             4687..7680
                     /locus_tag="VFV99_30065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063676.1"
                     /translation="MDFRRGVLLGTILLAACGDSPPGRTFYQRNIEPILLQKCAGNTS
                     GCHSTNVGDPYKFAAGNFDVTSFENVQKRRDVLERFGPYQQPLLLIKAVAPATPNPND
                     PNKLSVQYGKDGLGNDVFLPLDVLHAGGSIIDVSSDAYFTLQTWLENGATENGLKPPN
                     PAQKGNGGCSETLPPSFNASMYTSNANFGKFKSDVQPVLKDKGCTSTNCHGAPQSDFY
                     ITCGDNDDQIAFNFSQAWSFVNEPVDDSQLLRVPLAVAAGGRGHTGGDQFPSTDDGDF
                     IKFHDWAAAVKKLPFADTPEKTFFETNVQPLLVQRGCTFQACHSPAATNDFKLRSGTQ
                     GFFSAIALQRNYELLKQEFLALEFPDARRGRAIAKALLPDDYRVADQVGGIAHRGGAV
                     LETPGAANGSDPEQCPAWSGNTPPANVTAFCVIQEWITRERFAMAGSLTPMGQNDPVE
                     LVYVDRPSGQGADRLQFDTFQGGADLKHVTATFGANYGKNLAISGTAASLLTNCNGLT
                     AGTADVSAPDVANDGDRVAFAGRNSASGQLSIYIVRLSTGACTQAVTGMGHNFDPAWS
                     PDGKYLVFASTRSGGTTKKRNLPQSDLYRVEINASDAAVGTPERMTFLSSSEISPQFM
                     REGRVTMTTEKYSALAGQPEVYQLSGRRLNWDLTDYHPLLAQRAQSLYVAPGDMMTMN
                     ASIGFGSATDIREGSDGSFLLILTDVGMDGKPVTPGGGGALAIFNRSIGPFEQGRDDD
                     GYLPSVHIIDGGNASGRAGSQGAYRNPVTLPDGQIMASFATTSSLAWKLVAVNPRTGA
                     QTTIETPAGGRAIVDAVLAYRFPARPLYLNRRQLVFGGTSTTDSAHALVHFPDAPMVF
                     TLLTGNLRRGRPVDAFREATELVVKGADGNVLGKAALQDDGSAKIRVPSGTPVFLSLA
                     NGDKTVVQMTEEHQFGPGESISLGIRETFTNAAGRTVRLFDAVCGGCHGSVTGEELDV
                     TVSADALTGASVSASQNASPTNIGP"
     gene            complement(7683..9167)
                     /locus_tag="VFV99_30070"
     CDS             complement(7683..9167)
                     /locus_tag="VFV99_30070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063677.1"
                     /translation="MADLANILVVSDLHFGEELLPGATSERRNAVELGARAFRDFLRY
                     HAVRRLGGRPWRLVIAGDLFDFMSVVVPSTRELPARSADERRFGLDRGTRAGVYRMTA
                     ICENHRELLADMARFAAAGHFIDIIVGNHDLELLEPEVVAELMRQLQAAGIDVDESRP
                     RLLTASGPTWASRIRVLPWFVYEPGVAWIEHGHVYDEGCSSEYNLAPTDKDGRLIPNA
                     DYAATRYLAMAVPEIDPHGIEEWSFWGYMRYGWDVGMRSFGKLWAAYARFSVSLLKAH
                     VAQRSYKGRELRRRTHRERLRQIAEATNVAPETLSAIDRLARTPMTMSFRRLGRMLML
                     DRFGTYVGAFLALFMLWVFAPILWALLGTALVVGGAVALQSWLGKHHVTSQLPMRAVP
                     QRIKKLVNAPVIVFGHTHDPRWQPLRSGGLYLNTGTWLPATRPGLRRSFTHALIQPRP
                     DALPLVELRQWRDGVSQPFDAGADIGAGVTSPGLSDAMDWTKAV"
     gene            9290..11449
                     /locus_tag="VFV99_30075"
     CDS             9290..11449
                     /locus_tag="VFV99_30075"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5063678.1"
                     /translation="MTQLLFILLLAASVAFFARTMWLFGRALATPTPEPRSRIDELPG
                     RLMDVGVYFFGQKKVAEEGPQHKTSKHHLIIFWGFLIITIATADTVVSSAIPGLSLRL
                     LPDFMYLPLYAVIDVFNVLVLLMILWAYTRRIFVKPRLIPMNLDAGLILGAIGSLMVT
                     HFLMHGYEFAATGNGTWWMPISNALGKALAPLSETSAHRGAVIGYWLHVLVLLTFLNY
                     LPYSKHIHLLGALPNIFTRNRSERRMDLPKLNLEDENQWGVGRYEQFSWKSLLDTYAC
                     TECARCTNYCPAYNTGKNLSPMQLVHDIRYEMLDRTVLRDQIADAEKNVANLESFGKE
                     HGDDDAHPSPDLVFARNQLTALKAELESMPKMTGGRVQEDTLWACTTCGACQEVCPVF
                     IEHPLKILQMRQNLVLEQEKTPGELQRTYRNIERQSNPWGIANDQRMDWAKDLQVPTI
                     EEVPDPEYILWVGCAGAFDSRIIKQTRAMVKVLNHAGVSYAVLGHSEACTGDPARRTG
                     NEMMFQMLAEQNVETLKQANAKKVVTSCPHCLHTLKHDYPQFGGNFEVVHHTQLIDHL
                     YKENRLRSESSPVGKITYHDSCYLGRWNREFDAPRNILTSVGVPNVVEMTRSKRHGFC
                     CGAGGGRMFMEEHEGQRVNFNRTDEVIEAGVEAVAVACPFCNIMLTDGMKQRNVEDKI
                     QVLDVAELVAQSLPDVPVGNLVRKKDRDARAAAEATE"
     gene            complement(11458..14253)
                     /locus_tag="VFV99_30080"
     CDS             complement(11458..14253)
                     /locus_tag="VFV99_30080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5063679.1"
                     /translation="MKKSAMVVAIAVVLVLVFVIGRKQCGSKETTSTAPKPAATKQHA
                     TGLPASERKVAVAPWLAQLDIKPRKVAGKVVYEGKPVPGAIVRLGLPAAFDVLQPVAE
                     VKTGADGSFDFGPQPGTRYNVSAEAPQRTAAQVWIEVGDPRAKPDQLLLVLGGCESRL
                     FGSVLDASGGPVVKAHLRAAGLAGTETDAKGEYNLCVVSNFSAVRVEADGYGTINVGL
                     RLFGELRYDFVLVPEAVLVGQVITDDKKPVAGARVLAIPDPSEGPHHIASGWAFTEDD
                     GRFRIPGLSPGHYRVSAQAEGLASGAPVDAMVSAGTSAQEIVIIVGARARVSGRVMMG
                     DRAIAGARVVASLGQLVSDVAHSQDDGSFVLQGVPFGQVSFGAFPYEVTAPKQLEVKS
                     AKVDNVTLEVAAMASLHGIVTRKGKPSPDAELQGPQVPDPVKTDANGAYRIDGLAPGD
                     VNFAAWSVGSKAFTDVHVKVAAGEDKQLDIELQYAGEVKGTVVDESGKPVPNVYVRLL
                     NDEGDVGESMTNVRGEFDAGSMSGGEYLPSVYPSPMAGQPFQPVTGDHLDKITVPKDG
                     VVTGIKLAVKAERFTISGNVVDDKGGPVADVHIEAIGSGKGGIDLPSIMSTGDGTFTI
                     RNLARGMYTLHAHASDGSEADVLSVEAGAENVVVKMIRPGAIEGTLVGFTKPPMIQML
                     TLTSNLFIGGNPIVEGARFWQTGVRPGKYTVQAQAGSELGGESVEIRSGETAKVTITA
                     RGMGKVEGRVLEFGTKKPLAGFLCNSNLSLGGQMSGGPPDPARSSPSDEKGHFAMGAP
                     VGTVRVFCFPQGGGGPLTVAGTDVEVTAAAVPNVEVYSVRRVSSSEGNAGFRLRPLVL
                     PLVVNFVAPKSAAETQGMKVGDRLMSIDGASVQGLLPSPASTLIGNHKPGTTVTIGID
                     RGGQATIFKLPVTASPD"
     gene            complement(14270..16039)
                     /locus_tag="VFV99_30085"
     CDS             complement(14270..16039)
                     /locus_tag="VFV99_30085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063680.1"
                     /translation="MLALDLEETQEPPTTDETPVPRRPSEPLQPGDRYLLGETIAWGG
                     MGEVITAFDADIGRDVAIKRMRAPHPTPRALARFLREARIQGRLDHPAIVPVHELAYD
                     DDGRPFFVMKKLSGTTLLDILTKEPARFSRHQLLRAFADVCLAIEFAHTRGVVHRDIK
                     PANIVLGDFGEVYVLDWGIAKVEGDRERLQSEESSEDAKAMMTSPGLAIGTRGYMAPE
                     QARGHSVDHRADIYSLGCVLFEILTGHMLNPRDKRGAGAWSWVDARPALCAAGVAPEL
                     VELCVYATSHDREVRLGSARVLAETVQQYVDGDRDVVLRTRIARTHLDAALAADDDEA
                     GRRVAMREAGQALALDPTLKEAAELVGRLLIEPPQVMPREVAAELADLDRKAMPRHHW
                     QLAMMHSSFLALIPILAAFGVRDLFYATAALVLGCAGLALTTIQVRTNRNLVIPEHVI
                     VVLEFALLARAFTPFLVAPGVVAVNVIAFAHHPRAHRARTFWLCTVAGIVAVLAVVAA
                     EMLGFTSPTMITEQGHLTLHSPIDGFAAFPTLPALCIQFVIFVMSTAIVGRGTARLAH
                     ASRERIHLQAWQVRQLVPDRETR"
BASE COUNT         2874 a         5417 c         5298 g         2588 t
ORIGIN      
        1 cgccctacgt gctgaaggac ggcggcgtga tccgcgaagg ctacgacgcg accgtcgacg
       61 agtgtcgccg gcttgccgac ggcggcaagg acgagatcct cgcgatcgaa gcgcgcgagc
      121 agaaggacag cggcatcgcg aacctcaagg tccgctacaa ccgcgtgttc ggttactaca
      181 tcgagatcac gaagacgcac ctggcgaagg tgccggcgca ttacgtccgc aagcagacga
      241 tcgcgaccgg cgagcgctac gtgacgcccg agctcgccga gctcgagaag aaggtgctga
      301 cggccgagga aacgctggcg tcgcgcgaag ccgagctgtt caagaccgag gtcatggcgg
      361 tcgccgaggt cgcggcgaag atttcggcgg cgggtgccgc gctcgcgatc ctcgatgcgt
      421 gcgggtcgct tgcggatgtc gcggcgaagc gcgggtattg ccggcccgtt gtcgacgaca
      481 acttggtcat cgacctcgtc gacgcacggc atccggtgat cgaggcgctg ctaccaccgg
      541 gcagcttcgt accgaatgac tgccggctcg acccagcagc cgagcagcta gtgctgatca
      601 cgggcccgaa catggcgggc aagtcgacgt acatgcggca ggtcgcgcag atcgtgttgc
      661 tcgcgcagat cgggtcgttc gtgcccgcga aggcggcgac gatcggcgtg tgcgaccgcg
      721 tgttcacgcg cgtcggtgcg gcggataacc tgtcacgcgg cgactcgacg ttcatggtcg
      781 agatgcgcga gacgtcgtcg atcctcgcga aggcgaccaa gcgctcgctc gtcgtgctcg
      841 acgaggtcgg ccgcggcacg tcgacgttcg atggcgtgtc gatcgcgtgg gcggtcgccg
      901 aacatctgca cgacgcgatc ggcgcgcgca cgctgttcgc gacgcactat cacgagctcg
      961 tcgcactcgc tgatagtcgg ccgcgcgttc gcaacgtctc ggtcgcggta cgcgagcaca
     1021 agaacgagat cgtgttcctc cgtcgcgtca tcgctggtgg cgcgaacaag agctatggca
     1081 tcgacgtcgc caggctcgcg ggtctgcccc ggaacgtcgt caatcgggcg cgcgagatca
     1141 tggcgaagct cgaaggcggc aatcagttgg gttcgacgcc gcagctctcg ctgttgccga
     1201 cggcaccgcc ctcgcctgca ctcgcgcgca tcgctgcgat cgacatcgat cgcatgacgc
     1261 cactcgaagc gctgcagctg ctggcagagc tcaagtcgct gctctagatc gcgcgcgttg
     1321 cgaatctaga tgtagttatc gaaacgacgt gtgtttgggg gccaagagcc acacgcggtc
     1381 gaaatagggg gcgctagccc cgacaactcc cgacgacaac cgcaagcatt ccaatcggtt
     1441 cagcttggcc tgcgctttgt acttagtggc cgccatgagg aacatcaaga gcatcgcgat
     1501 cggagccgcg ttgttcactt ccgtgttcgg cgggggatgc ggcaacagtg acgactcggg
     1561 gccgctcggc aacgtcgaca gcctcgtcat cctgcagcgc cccaagcgca acgacatggg
     1621 tgacatcttc caatacacct cgtacgtgcc gacggcgcgg cttgtaaagc tgtcgccgcc
     1681 gactgcggac ggtacgctga cgacgctctg ctgtgacaag gcaggcgccg agttcgcgga
     1741 catcgacatt tcgtcgtacg acatctcgtt cgacgcgaag gagatcgtgt tctcgggcaa
     1801 gctgaagggc agtgaccggt acggcttgtt ccggctgacg ctcgagagcg gcgcggtcac
     1861 gcagatcgcg accgaccccg gccgcgacta cgtcaacccg atctacctgc cgggtgacaa
     1921 ggtgatgttc cagacgaacc tcgccgttaa caacatggcg atggatccgg tggccaacac
     1981 gccgatcgac gccaacccgg cgcagcacgt cgacgagtac gagcgcggta ccacgctcca
     2041 gatgggcacc gtcggactcg acggcaccgg cgaggtgctt ggcccacgta acctctcgca
     2101 ccgtacgttc ccgacgctca ccagcgacgg gcgcgtgatg ttcacgcagt gggaccacct
     2161 cggtccggtc aacgctggcc acctcatgtt catgaacacg gacatgacgg agctgcgtga
     2221 agcattcggc aaggaaggca ccggcgcctc gaactcgacg ctcaaggcgc gcgagatcag
     2281 cccgggccgc ttcgtcggta tcgcgacgtc gcgtgaccgc acgatccagt cgggcgcgct
     2341 catcgacatc cgcctcggta tgccttcgac gaagaacggc gtgctccgcg ccgacacggc
     2401 gatgagcgag gcgaacgcga gctacgtcat gctgacgcct gacgtcccga ccgaccgcga
     2461 gccgagcgcc aacacgatcg gccgctacta cgacgcattc ccgctcaacg cgggtgagca
     2521 tcccgacctg ctggtctcgt gggccgacgg tccggtcgag tcgagcgtgc tcggctcggc
     2581 gggcatctcg gcgaacttcg gcgtctacct ctacgacacc aagacgctgc agcgtcaccc
     2641 gatcctcgac gacccggaga tgtgggacat cttcgcgcgt ccgctgatgc cgcgttcggc
     2701 gccggcggtg acgagctcgt cgatcgacaa cagcctcggt ggccaagcga tggtcggcgc
     2761 gatgaacgtg tacgagtcga gcctgaccac gttccagccg ggctcgatct acggcgtccg
     2821 catcatggaa ggcttctcgt cggaagaagg cttcccggag gacttcggca ccacgatgtt
     2881 tgaaggccag gccaacctcg gcgtcgctcg cgtggcgtcg gacggcagct ggctcgccac
     2941 cgtgccggcg aacgtgccgc tccacctgca agcgatcgac gtcttcggta tgtcgcgcga
     3001 gaacgagccc gtgtggttca gcgcgcgtcc cggtgagtcg cgcgtctgcg gtggttgcca
     3061 cgagaaccgc gccaccacga ccgtcatcaa ccccggtatc acgcaggccg ctgccatcgg
     3121 cccgaccgac gcgaagggcc ttgtctcgcg cgcccgccgc atctcgacgg atgccgagct
     3181 gtcgtcgcca accactgcgc cggaccgcct cgtcggtatg gcgtggaaca aagccatcca
     3241 gccggtcttc gacgcgaagt gcgtcagctg ccacgacggt acgccgagcg cggcgaaccc
     3301 gacctaccag atcgtcgacc cgacgaccgg cacgatggtc gcgcagttca cgttcaacct
     3361 gacggcgacg ccggtgtcga tcgactacgg catgggcgaa gtccaggagt ggcccgcgtc
     3421 gtacctgtcg atggcgggtc ccgacatgga agccatcgag atgggcaacc tgatggtgac
     3481 cggcacgctg ccgtgcaaga ccgtcggtgg ccagcccacg ctccagtgca tggagccgca
     3541 gaactcgcgt gactcgtacg tcatccgcct cgtcaacccg acccagctgt tcccgacccc
     3601 gagcacgacg cgtgcgttca acacgtcgcc gcactcgagc gtgggttaca cggagctgac
     3661 gtcggccgag ttctacaagc tgatcctcgc ggctgacatg ggcgtcaact actacgcccg
     3721 cgagaacaac ccgatgctca atcggtacta accccaaccg cggaaaagga gaaacaccat
     3781 gaacatctct aagaaagtcg ccgcggtcgc cttcgtcctg ctttgtggag cagcgccggc
     3841 gttcgcaggt aaaggtggca gcaacgcgaa aatcgttgcg gccgtgcaaa gtggctcggt
     3901 cgacgcgatc accgcggaag tcgagcgggc agaaggtctc atgtgccccg agtgcgtggg
     3961 caccgtgctg aacctcctcg aggacaaccg gttcgcggta cgtgaagtcg ccgcgtggtg
     4021 gttcgcgaag cggccgggcg cgaaggacca gctcgccacg cagttcaagg ccgagctctc
     4081 gacctcgggc tcgatcgctg tgcgcaacgc cgccgacttc ctcggccgcg tgcgccagtt
     4141 cgatgcgctg cctcagctgc gcgtcgcgat caagcgcacc gacatctccg cagaagcaaa
     4201 gctcgcgatc gttcgcgcag tcggttacat ggcgcaccct gacggcaaca gcgtgctcct
     4261 gaccgcgatg aacgacggcg acgcgagcgt gcgcgctgcg gcggtgacgg cgtggcgcga
     4321 gatcctcggc cagctcgacg tcaaaccact cgtcagcaag ctcgctgact ccgacgcaag
     4381 cgtgcgcgct gcggcggcga tggtggtcgg cgtctacggc gagccgagcg cgcgtacgca
     4441 gctcgagcaa ctcgtaacga cggacaccga ttcgttcgtg cgtcgcaacg cggcgtgggc
     4501 gctgggtcgc atcggcgaca acgcgtcggc gcaagcgctg cagaccgcaa cgaacgacaa
     4561 gtcgggcatc gtccgtggct acgcgtcggc ggcactcgcc gagctcaagt agtcgcaatc
     4621 ttcctctgtg atcctaagtg agccacgtcg cgcaacgacg tggctcattt gcgataggat
     4681 tcaattatgg actttcgaag gggcgtcctc ctcggaacca ttctgctcgc ggcgtgcggt
     4741 gactcgcccc ctgggcgaac gttctaccaa cgcaacatcg agccgatcct gctgcagaaa
     4801 tgcgcgggca acacgtcggg ctgccactcg acgaacgtcg gcgatccgta caagttcgcc
     4861 gccggcaact tcgacgtcac gtcgttcgag aacgtgcaga agcgacgtga cgtgctcgag
     4921 cgcttcgggc cgtatcaaca gccgctgctg ctgatcaagg ccgtcgcgcc ggcgacgccg
     4981 aacccgaacg atccgaacaa gctctccgtg cagtacggca aggacggcct cggtaacgac
     5041 gtgttcttgc cactcgacgt cctgcacgcc ggcggctcga tcatcgacgt gtcatccgac
     5101 gcgtacttca cgctgcagac gtggctcgag aacggcgcga ccgagaatgg tctcaagccg
     5161 ccgaacccgg cgcagaaagg aaacggcggc tgctcggaaa ccttgccgcc cagcttcaac
     5221 gcgtcgatgt acacgagcaa cgcgaacttc ggaaagttca agagcgacgt ccagccggtc
     5281 ctcaaggaca agggttgcac gtcgacgaac tgccacggcg cgccgcagtc ggacttctac
     5341 atcacgtgcg gcgacaacga cgaccagatc gccttcaact tcagccaggc gtggtcgttc
     5401 gtgaacgagc ccgtcgatga ctcgcagctg ctacgcgtac cgcttgcggt cgcagctggc
     5461 ggccgcggtc acaccggcgg ggatcagttc ccgagcaccg atgacggtga cttcatcaag
     5521 ttccacgatt gggcagccgc ggtgaagaag ctgccgtttg ccgacacgcc ggagaaaacg
     5581 ttcttcgaga ccaacgtgca accgctgctc gtccagcgcg gctgtacgtt ccaggcctgc
     5641 cacagcccgg ccgcgacgaa cgacttcaag ttgcgctcgg gtacgcaggg cttcttctcc
     5701 gcgatcgcgc tgcaacgcaa ctacgagctc ctcaagcagg agttcctcgc gctcgaattc
     5761 ccggacgcgc gccgcggtcg tgccatcgcg aaggcgctgc tccccgatga ctatcgcgtc
     5821 gccgatcaag tcggcggcat cgcgcaccgt ggcggcgccg tactcgaaac gcccggcgcc
     5881 gcgaacggct ccgatccgga acagtgtccc gcgtggagcg gcaacacgcc gcccgcgaac
     5941 gtcaccgcgt tctgcgttat ccaggaatgg atcacgcgcg agcgcttcgc gatggcgggc
     6001 tcactgacac cgatgggcca aaacgacccc gtcgagctcg tgtacgtcga caggccctcg
     6061 ggccaaggcg ccgaccgcct ccagttcgac acgttccaag gcggcgctga cctcaagcac
     6121 gtgaccgcga cgttcggcgc gaactacggc aagaacctcg cgatcagcgg caccgccgcg
     6181 tcgctgctca cgaactgcaa cggtctcacc gcgggcaccg ccgacgtctc ggcgcccgac
     6241 gtcgcgaacg acggcgatcg cgtcgcgttc gcaggccgca acagcgcgtc gggccagctc
     6301 tcgatctaca tcgtgcggct gtcgaccggc gcctgcaccc aggccgtcac gggtatgggc
     6361 cacaacttcg atccggcgtg gtcgcccgac ggcaagtacc tcgtgttcgc atcgacccgc
     6421 agcggcggca cgacgaagaa acgcaacctg ccgcagtcgg atctctatcg cgtcgagatc
     6481 aacgcgtcgg acgcggcggt cggcacgccc gaacgcatga ccttcctctc cagctcggag
     6541 atcagtccgc agttcatgcg cgaaggccgc gtgacgatga cgaccgagaa gtacagcgcg
     6601 ctcgccggtc agcccgaggt ctaccagctc agcggtcgcc gcttgaactg ggacctcacc
     6661 gactaccacc cgctgctcgc gcagcgcgcg cagtcactct acgtcgcgcc cggcgacatg
     6721 atgacgatga acgcgtcgat cggtttcggc tcggcgaccg acatccgcga aggttccgac
     6781 ggcagcttcc tgctcatcct caccgacgtc ggcatggacg gcaaacccgt cacgcccggc
     6841 ggcggtggtg cgctcgcgat cttcaaccgc agtatcggcc cgttcgagca aggccgtgac
     6901 gacgatggct acctgccctc cgtgcacatc atcgacggcg gcaacgcctc gggtcgcgcg
     6961 ggctcgcaag gcgcctatcg caatccggtc acgctgcccg acggtcagat catggcgtcg
     7021 ttcgcgacga cgtcgtcact cgcgtggaag ctcgtcgcgg tgaacccgcg caccggcgcg
     7081 caaaccacga tcgagacgcc cgccggtggt cgcgcgattg tcgacgcggt gctcgcctac
     7141 cgcttcccgg cgcgcccgct gtacctcaat cgccgccagc tcgtgttcgg cggcacgtcg
     7201 accaccgact ctgcacacgc gctcgtccac ttccccgacg cgccgatggt gttcacgctc
     7261 ctcaccggca acctgcgccg cggccggccg gtcgacgcat tccgcgaggc aaccgagctc
     7321 gtcgtcaagg gcgcggacgg taacgtgctc ggcaaagcag ctcttcagga cgacggctcg
     7381 gcaaagatcc gcgtgccctc gggaacgccc gtgttcctct cgctcgcgaa cggagacaag
     7441 accgtcgtac agatgaccga ggaacaccag ttcggccccg gcgagtccat ctcgctcggc
     7501 atccgcgaga cgttcacgaa cgccgccggc aggaccgtgc gcctgttcga cgccgtgtgc
     7561 ggcggctgcc acggatcggt gacaggcgag gagctcgacg tcacggtcag cgcagacgcg
     7621 ctcaccggcg cctcggtgtc ggcgagtcag aacgcgtcac cgacgaacat cggcccgtag
     7681 gactaaacgg ccttcgtcca gtccatcgcg tcggacaagc cgggtgacgt gacgcctgcg
     7741 ccgatgtcgg cccctgcgtc gaacggctgg ctcacgccgt cgcgccactg gcgcagctcg
     7801 acgagcggaa gcgcgtcggg ccgcggctgg atgagcgcgt gcgtgaagct tcggcgcaaa
     7861 ccagggcgcg tcgccggcag ccaggtgccg gtgttgagat agaggccgcc ggagcgcagc
     7921 ggttgccagc gcggatcgtg ggtgtggccg aacacgatca caggcgcgtt gacgagcttc
     7981 ttgatgcgct ggggaacggc gcgcatcggc agctgcgacg tcacgtgatg cttgccgagc
     8041 caggactgga gggcgactgc gccgccgacg acgagggcgg tgccgagcag cgcccacagg
     8101 atcggtgcga agacccagag catgaacagc gcgaggaacg cgccgacgta ggtgccgaag
     8161 cgatcgagca tcagcatgcg gccgagccgg cggaagctca tcgtcatcgg cgtgcgtgcg
     8221 aggcgatcga tcgcggagag ggtttcgggt gcgacgttgg tggcttcggc gatctggcgc
     8281 aggcgctcgc ggtgcgtgcg gcggcgcagc tcgcgacctt tgtacgagcg ctgcgcgacg
     8341 tgggctttga gtagcgacac cgagaagcgc gcgtaggcgg cccacagctt gccgaagctg
     8401 cgcatgccga cgtcccaacc gtagcgcatg tagccccaga acgaccattc ctcgatgccg
     8461 tgcggatcga tttcggggac cgccatcgcg aggtagcggg tggcggcgta gtccgcgttc
     8521 gggatgaggc ggccgtcctt gtcggtcggt gcgaggttgt actcggagga gcagccctcg
     8581 tcgtagacgt ggccatgctc gatccatgcg acgcccggct cgtacacgaa ccacggtagg
     8641 acgcgaatgc gcgaggccca ggtgggaccg ctggcggtga gtagccgcgg ccgcgattcg
     8701 tcgacgtcga tgccggccgc ctggagctgg cgcatcagct cggcgacgac ctcgggctcg
     8761 agcagctcga ggtcgtggtt gccgacgatg atgtcgatga agtggccggc ggccgcgaag
     8821 cgcgccatgt cggcgagcag ctcgcggtga ttctcgcaga tcgcggtcat gcgatagacg
     8881 ccggcacgcg tgccgcgatc gagaccgaag cggcgctcgt cggcggagcg cgcgggaagc
     8941 tcgcgcgtcg acggtacgac gacggacatg aagtcgaaga ggtcgcccgc gatcacgaga
     9001 cgccatgggc ggccgccgag gcgacgaacg gcgtggtagc ggagaaagtc gcggaacgca
     9061 cgcgcgccga gctcgacggc gttgcggcgc tcggatgtcg cgccagggag gagctcctcg
     9121 ccgaagtgaa ggtcgcttac gaccaggatg ttcgcgaggt cggccatgac gagactggtt
     9181 ctgacaccat gacgggtgga gctgcatcag acaagaatgc aggtcacgga cttaagatcc
     9241 cgtggtgcca tgacaatctg gcagtttgca tggtaagccc gaacccgtcg tgactcagct
     9301 cttgttcatt ctgctgctcg cggcgtcggt ggcgtttttc gctcgcacga tgtggctgtt
     9361 tggccgcgcg ctagcgactc caacgccgga accgcggtcg cgtatcgatg agcttcccgg
     9421 ccgtctgatg gacgtcggcg tgtacttctt tggccagaag aaggtggcgg aggaaggtcc
     9481 gcaacacaag acgagcaagc accacctgat catcttctgg gggttcttga tcatcacgat
     9541 cgcgacggcc gacaccgtcg tgtcgagtgc gattcctggg ctgtcgctcc ggctcttgcc
     9601 ggacttcatg tatctgccgc tctacgcggt catcgacgtg ttcaacgtgc tcgtgctgct
     9661 gatgatcttg tgggcctaca cgcgccgcat cttcgtcaag ccgcgcttga tcccgatgaa
     9721 cctcgacgcc ggcctcatcc tcggcgcgat cggctcgctg atggtgacgc acttcttgat
     9781 gcacggctac gagttcgccg cgaccggcaa cggcacgtgg tggatgccga tttcgaacgc
     9841 gctcgggaag gcgctcgcgc cgctctcgga gaccagcgcg catcgcggcg cggtgatcgg
     9901 ctactggctg cacgtgctcg tgctgctcac gttcttgaac tacctgccct actccaagca
     9961 catccacttg ctcggcgcgc tgccgaacat cttcacgcgc aaccgcagcg agcggcggat
    10021 ggacctgccg aagctgaacc tcgaggacga gaatcagtgg ggcgtcggtc gctacgagca
    10081 gttttcgtgg aaaagcctgc tcgacaccta cgcgtgcacc gagtgcgcgc gctgcacgaa
    10141 ctactgcccc gcgtacaaca ccggcaagaa cctgtcgccg atgcagctcg tgcacgacat
    10201 ccggtacgag atgctcgatc gcacggtgct gcgcgaccag atcgccgacg ccgagaagaa
    10261 cgtcgcgaac ctcgagtcgt tcggcaagga gcacggcgac gacgacgcgc acccgagccc
    10321 cgatctcgtg ttcgcgcgca atcagctgac cgcgctcaag gccgagctcg agagcatgcc
    10381 gaagatgacc ggcggccgcg tgcaggaaga cacgctgtgg gcgtgcacga cctgtggcgc
    10441 atgccaggaa gtctgcccgg tgttcatcga gcacccgctc aagattctgc agatgcgcca
    10501 gaacctggtg ctcgagcagg agaagacgcc cggcgagctg cagcgcacgt atcgcaacat
    10561 cgagcgccag agcaatccgt ggggcatcgc gaacgatcag cgcatggact gggcgaagga
    10621 cctccaggtt ccgaccatcg aggaggttcc cgaccccgag tacatcctct gggtcggctg
    10681 cgccggcgca ttcgacagcc gcatcatcaa gcagacgcgc gcgatggtga aggtgctcaa
    10741 ccacgcgggc gtgagctacg cggtgctcgg gcacagcgaa gcgtgcaccg gtgatccggc
    10801 gcgccgcacc ggcaacgaga tgatgttcca gatgctcgcc gagcagaacg tcgagacgct
    10861 gaagcaggcg aacgcgaaga aggtcgtcac gagctgcccg cactgcctgc acacgctgaa
    10921 gcacgactac ccgcaattcg gcggcaactt cgaggtcgtg caccacacgc agctgatcga
    10981 tcacctctac aaagagaatc ggctgcgcag tgagagctcg cccgtcggca agatcaccta
    11041 tcacgacagc tgctatctcg gccgctggaa ccgcgagttc gatgcgccgc gcaacatcct
    11101 tacgagcgtc ggcgttccga acgtagtcga gatgacgcgc agcaagcgcc acggcttctg
    11161 ttgcggcgcc ggtggcggcc ggatgttcat ggaagagcac gagggtcagc gcgtgaactt
    11221 caaccgcacc gacgaggtca tcgaggccgg cgtcgaggcg gtcgcagtcg cctgcccgtt
    11281 ctgcaacatc atgctcaccg acggcatgaa gcagcgaaac gtcgaggaca agatccaggt
    11341 cctcgatgtc gccgagctcg tcgcgcagtc gttgcccgac gtgccggttg gcaatctcgt
    11401 gcgcaagaaa gaccgcgacg cacgcgcggc cgccgaagcc accgagtgat gtaacggtca
    11461 gtctggcgac gcggtgaccg gcagcttgaa gatggtcgcc tggccgccgc gatcgatgcc
    11521 gatcgtcacc gtcgtgcccg gcttgtggtt gccgatcaac gtcgacgcgg gcgacggcaa
    11581 gaggccctgc accgacgcac catcgatcga catcagacga tcgccgacct tcatgccttg
    11641 tgtctcggcg gcgctcttcg gcgcgacgaa gttgacgaca agcggcagca cgagcgggcg
    11701 aagccggaag cccgcgttgc cctcagacga gctgacccgg cgcaccgagt agacctcgac
    11761 gttcggcacc gcggcggccg tgacctcgac gtcggtgcct gcgaccgtta acggaccgcc
    11821 gccgccctgc gggaagcaga acacgcgcac ggtgccgacc ggcgcgccca tcgcgaagtg
    11881 gcccttctcg tcggatggcg aggaccgtgc cggatcgggc ggaccaccgc tcatctgacc
    11941 gccgagcgac aagttcgagt tgcagagaaa cccggccagt ggtttcttcg tgccgaactc
    12001 gagcacgcga ccctcgacct tccccatgcc acgggccgtg atcgtgacct tcgcggtttc
    12061 gccggaacga atctcgacgg actcgccacc gagctcacta ccggcctggg cctgcaccgt
    12121 gtacttgccc gggcgcacgc cggtctgcca gaaccgcgcg ccctcgacga tcggatttcc
    12181 gccgatgaac aggttcgagg tcaacgtcag catctgaatc attggcggct tcgtgaagcc
    12241 gacgagcgtg ccctcgatcg cgccggggcg gatcatcttg acgacgacgt tctcggcgcc
    12301 cgcttcgaca ctgagcacat cggcctcgct gccgtcgctc gcatgcgcat ggagcgtgta
    12361 catgccacgc gcgaggtttc ggatcgtgaa cgtgccgtcg ccggtgctca tgatcgatgg
    12421 caggtcgatg ccgccctttc cgctgccgat tgcttcgatg tggacgtcgg cgaccggacc
    12481 acccttatca tcgacgacgt ttccgctgat cgtgaagcgc tctgctttca ccgcgagctt
    12541 gatgccggta acgacgccat ccttcggcac cgtgatcttg tcgaggtgat cgccggtgac
    12601 cggctggaac ggctggcccg ccatcgggga cggatacacg ctcggcaggt actcgccgcc
    12661 cgacatcgag ccggcatcga attcaccgcg cacgttcgtc atcgattcgc cgacgtcgcc
    12721 ctcgtcgttg agcaggcgga cgtagacgtt cggcactggc ttgccggact cgtcgacgac
    12781 cgtacccttg acctcgcctg catactgcag ctcgatatcg agctgcttgt cctcgcccgc
    12841 ggcgaccttc acgtggacgt cggtgaacgc cttcgacccg acgctccacg cggcgaagtt
    12901 cacgtcgccc ggcgcgaggc cgtcgattcg atacgcgccg ttggcgtcgg tcttcaccgg
    12961 gtcgggaacc tgcggccctt gcaactcggc gtcgggggac ggcttgccct ttcgcgtgac
    13021 gatgccgtgg agcgacgcca tcgccgcgac ctcgagcgtc acgttgtcga cctttgcaga
    13081 cttcacctcg agctgcttcg gcgcggtcac ctcgtacgga aacgcgccga agctgacctg
    13141 cccgaacggc acgccctgca acacgaagct gccgtcgtcc tgcgaatgcg cgacatcgct
    13201 gacgagctgg ccgagactcg ccacgacgcg tgctcccgca atcgctcggt cgcccatcat
    13261 cacgcggcca ctcacgcgcg cgcgggcccc aacgatgatg acgatctcct gcgccgacgt
    13321 gccggcgctc accatcgcgt cgacgggcgc gccggatgcg aggccttcgg cctgtgcact
    13381 gactcggtag tgtccgggcg acaggccggg aatgcggaag cggccgtcgt cctcggtgaa
    13441 cgcccagccg ctcgcgatgt gatgcgggcc ttcgctcgga tcgggaatcg cgagcacgcg
    13501 tgcgccggcg accggcttct tgtcatcggt gatcacttgg ccgacgagca cggcctccgg
    13561 cacgagcacg aagtcgtagc gcagctcgcc gaatagccgc agaccgacgt tgatcgtacc
    13621 gtagccgtcg gcctcgacac gcacagccga gaagttcgag acgacgcaga gattgtactc
    13681 gcccttcgca tcggtctcgg tgccggcgag ccccgccgcg cggagatgag ccttgacgac
    13741 gggcccaccg ctcgcgtcga gcacggagcc aaacagccgc gactcacacc cgccgagcac
    13801 gaggaggagc tggtcgggct tcgcgcgcgg atcgccgacc tcgatccaga cctgcgctgc
    13861 ggtgcgctgc ggcgcctcgg cgctgacgtt gtagcgtgtg cccggctgcg ggccgaagtc
    13921 gaacgagcca tcggcgccgg tcttcacctc ggcgaccggt tgcagtacgt cgaaggccgc
    13981 tgggagcccg agccgaacga tggcaccggg aaccggcttg ccctcgtaca cgaccttgcc
    14041 ggcgaccttg cgcggcttga tgtcgagctg cgcgagccac ggcgccacgg cgaccttgcg
    14101 ctcgctcgca ggcaggcctg tcgcgtgctg cttcgtcgcc gcgggctttg gcgctgtgct
    14161 cgtcgtctct ttactgccgc actgcttccg cccgatcacg aagaccagga cgagcaccac
    14221 ggcgatggcc accaccatcg cggacttctt cattgcaacc gagcatacgt caacgtgtct
    14281 cgcgatcggg aacaagctgc cggacctgcc acgcttgcag gtgaatccgt tcacgcgatg
    14341 cgtgcgcgag gcgcgcggtg ccgcgtccga cgatcgccgt cgacatcacg aagatcacga
    14401 actggataca caacgccggc agcgtcggga acgccgcgaa gccgtcgatc ggcgagtgga
    14461 gggtcaagtg accttgctcg gtgatcatcg tcggcgacgt gaacccgagc atttcagccg
    14521 caaccaccgc gagcacggca acgatgccgg cgaccgtaca cagccagaac gtgcgcgcgc
    14581 ggtgtgcgcg cggatgatgc gcgaacgcga tcacgttgac cgcgacgacg ccgggcgcga
    14641 cgaggaatgg tgtgaatgcg cgcgcgagca gtgcgaactc gaggacgacg atcacgtgct
    14701 cggggatcac gaggttgcga ttcgtgcgga cctggatcgt cgtgagcgcg aggcccgcac
    14761 agccgagcac cagcgccgcg gtcgcgtaga acagatcgcg cacgccgaac gcggccagga
    14821 tcggaatcag cgcgaggaac gacgagtgca tcatcgcgag ctgccaatga tggcgaggca
    14881 tcgccttgcg atcgagatct gccagctcgg ccgcgacctc gcgcggcatc acctgcggcg
    14941 gctcgatcaa cagccgaccg acgagctcgg ccgcttcctt gagcgtcgga tcgagtgcga
    15001 gcgcctgccc tgcttcgcgc attgcaacgc ggcgccccgc ttcgtcgtca tcggcagcga
    15061 gcgcagcgtc gaggtgcgtg cgtgcgatcc gcgtgcggag cacgacgtcg cgatcgccgt
    15121 cgacgtactg ctgcacggtc tcggcgagca cgcgcgcgga gccaaggcgc acctcgcgat
    15181 catgcgacgt cgcgtagacg catagctcga cgagctcggg cgcgacaccc gccgcgcaca
    15241 atgccggccg cgcatcgacc cacgaccacg caccggcgcc gcgcttgtcg cgcgggttca
    15301 gcatgtgacc ggtgaggatc tcgaacagca cgcagccgag cgaatagatg tcggcgcggt
    15361 gatcgacgct atgcccgcgc gcttgctcgg gcgccatgta gccgcgcgtg ccgatcgcca
    15421 aacccggcga cgtcatcatc gccttcgcgt cttcgctcga ctcctccgac tgcagacgct
    15481 cgcgatcgcc ttcgactttc gcgatgcccc agtcgagcac gtagacttca ccgaaatcgc
    15541 cgagcacgat gttcgccggc ttgatgtcgc gatgaacgac gccgcgggtg tgcgcgaatt
    15601 cgatcgcgag gcagacgtcg gcgaacgcgc gcagcagctg atgccgcgag aaccgcgccg
    15661 gctctttcgt gaggatgtcg agcagcgtcg tgccgctgag cttcttcatc acgaagaacg
    15721 ggcggccatc gtcgtcgtag gcgagctcgt ggaccgggac gatcgcgggg tgatcgaggc
    15781 gaccctgaat ccgagcttct cgcaggaagc gagctagcgc acgcggcgtc gggtgcggcg
    15841 cgcgcatgcg tttgatcgcg acgtcgcggc cgatatcggc gtcgaacgcg gtgatcacct
    15901 cgcccatccc gccccaggcg atcgtctcgc cgaggaggta gcggtcgcct ggctggagcg
    15961 gttccgacgg ccgacgcgga acgggtgtct cgtcggtggt cggcggctcc tgggtctctt
    16021 ccaggtcgag cgccagcacg cgggccacct atcgcaagcc gagtgccagc ggtttttgcg
    16081 atcaagtgcg ctttcttgcg caactgacct gggaccaact accccggagc tgagggatca
    16141 ggcgtaaccc gcgttatgat gctcacgatg aagggct
//
LOCUS       DASUFX010000746        15530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1508032, whole genome shotgun sequence.
ACCESSION   DASUFX010000746 DASUFX010000000
VERSION     DASUFX010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508032"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFV99_30090"
     CDS             <1..902
                     /locus_tag="VFV99_30090"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5063681.1"
                     /translation="LDAGAIVFPQPASEWAIENAGALIESGCAIVRLRNVVEVPFAGL
                     AVAARDRYFAQDARDRLRSLIEAHWTRTISNTDFARWAQTLIARVVGSEQARSSIVMP
                     PGGATPIGTLGAMSATFELADQVAAGIAPPPRRIVLPVGSTCTTAGLISGFALAHAIG
                     VWRWPVPIVHAVRVTPWPVTSRVMITRLAAFTLARIAQLGGPRVELGDARLVIDAREL
                     GAGYGRITKRGTQAMSRFAHDSAPRLDGVYSAKAAAALLRLHREGVGPLVFWSTKSTT
                     QLAPPSRPAIARAPRALARWFAS"
     gene            complement(932..2365)
                     /locus_tag="VFV99_30095"
     CDS             complement(932..2365)
                     /locus_tag="VFV99_30095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063682.1"
                     /translation="MDRQDIDALLIGALYGELTPADEARLAAHLDSHPTDRGALDDLK
                     SARNAVRESRIFELQVDPPQAVSALLLQEAHRRAPKRAPEREHEGNEGWFYRFSRMFL
                     AHPAMAAAAMLVLVVGVAGTLYMKKGSDQFVDKQAEPPALQEKADTVAVEAPAMDPAA
                     ANEGVGAADTGIAQGSGSSFGYQVQLADGKAEAKQQASDRAEQVAKLKDQQNALERQQ
                     ELAVAKNKRAASSSIIVDTPQREPKELDEPRAKVASKVGAKKGNVQGGEDAEIATDSF
                     DSNRGRGDTAATGSTVARAGAGGGGTVGGATGTGAPGGRVATTPPAAAGPARNAPTPA
                     PPPPPAATTAPAPAQVAQQQPKSPTATAKPTTPPAKVAKEAEKTESKPDSTAGKDSAL
                     IAWAKGQHSQAQSLAGKGDCAGAAKVAVLISNRAPEFYSQYVATDRALKKCQAYIAAE
                     RDADAERSGKARSQKRVNADQPAEAAH"
     gene            complement(2565..2882)
                     /locus_tag="VFV99_30100"
     CDS             complement(2565..2882)
                     /locus_tag="VFV99_30100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063683.1"
                     /translation="MPVLAKGSHAKKSLSDCTSFTQKEKGDDAFEMNVHNSCKAQVDC
                     SISWKVICSPDSQKRRAVHASSQKFTLLEGGEQTAEASAAVCGDEAWTIESVQWGCEP
                     AKD"
     gene            3071..3874
                     /locus_tag="VFV99_30105"
     CDS             3071..3874
                     /locus_tag="VFV99_30105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5063684.1"
                     /translation="MQSPLDVFRPGVLDGHVALVTGGGTGICRGIAAAYARFGADVCI
                     VSRKQDVLEKTAAELSAASGRQVLAVAADVRQPDDVARAVAQTVERFGKLDTLVNGAA
                     GNFLAPAAGLSPNGFRTVIDIDLVGTFHAARAAFEQLRASGRGLVLNISATLHYHGTP
                     LQIHASAAKAGVDAITKNLAVEWGQFGIRSCGIAPGPIGDTEGMKRLAPGDIAKKAMA
                     GIPAGRFGTIDEIAAAAVYLRSPAAAYVSGHVLVVDGGHCVSTPSLMGV"
     gene            3874..4533
                     /locus_tag="VFV99_30110"
     CDS             3874..4533
                     /locus_tag="VFV99_30110"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX5063685.1"
                     /translation="MIDLVAPARPVAMELVSGRGHYDRVLAAVLGAKVSVWIATANVK
                     ELMVEDHRARPGRRRSLRKANYVSVLSHLDELAARGVELRLLHAELPSRPFREELAQH
                     PKLVAGGLALRRCPRVHLKTVIVDGELLYLGSANWTGAGLGAKGSGRRNFELGIVTDD
                     AQLLDHIQALYEQIWTGGECATCKLQDECPGPLVQLGRAPATPPVPTKRVRKTRKKPR
                     R"
     gene            4578..5186
                     /locus_tag="VFV99_30115"
     CDS             4578..5186
                     /locus_tag="VFV99_30115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5063686.1"
                     /translation="MTVAAAYVEDVLLVDRCLTGEPAATRELFRRHRGRVHASLFRVL
                     GSNRDMDDLLQEAFIQVFQSLRGWRAEASLSTWIDRVSVRVAYRYLSQRGRRIQTDPM
                     IDDDNVPGGEEPGARRQLARDGIKRLYTVLDELGAASRLAFSLHEIDGRPLAEVAELV
                     GSSVTATKLRVWRARKRVLEAAASDPILSEFIEPGFLAKESA"
     gene            5183..6349
                     /locus_tag="VFV99_30120"
     CDS             5183..6349
                     /locus_tag="VFV99_30120"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX5063687.1"
                     /translation="MNLVGKVPVEPLDEERLTNIERKLVVQVSEMSQRPHRAPRRGFA
                     YAGFAMAVAIAGFVGWKLHRDPVVEPAKPEQLAMKAGALDLGDAQITGSDFDITRTAA
                     LTMVVMHPGKLDLHVEHKPGRVFVVQAGDVEIEDIGTRFTVDYDGKNVDVRVTEGEVK
                     VKRAGKELRVTAGNAWTLEIGPITIAQLTAQQAQTVGATNPQPQATAQNNVVAMAPTN
                     NAGSGSGVGSAQGSGASRPAKTPQSNAKRAIDNLPLEPLVATDLKDPKAAIAEYLKTV
                     RDMPEGEDKAELLQSIAVMQLRQKDLDGAAHSIKGILQRQGGDTPAYKQALWLDMRIK
                     CATSYDRDNNRDGKADRLFDDDCRRSAERYIVKFPTGAAAGVAQEVLNEISRGQ"
     gene            6447..7298
                     /locus_tag="VFV99_30125"
     CDS             6447..7298
                     /locus_tag="VFV99_30125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063688.1"
                     /translation="MSRRTVVLELAGEHQLARDLVAGGVFVPGEVLAIGEDCDLLLRG
                     EEGELALHATCVWVDREKGSGLQLMGCDAEMKQQIATLAVGTQLEDIATSEVERVDIT
                     LDDMTDDDTEESQYEADGPSAEELAAAELEAELAGDDEEDAERKLALNVNERLRGLTL
                     VQQLRVAQRGELSERIVLERMYGKNVWDALLRNQRLTGPEVARIARMGALPRPLIELI
                     VGNGAWLQIPEVRRALLSNPRLATDQISRVLRLLPKHELKLASVQTAYPFAVRDAAKR
                     MLREMSG"
     gene            7495..8214
                     /locus_tag="VFV99_30130"
     CDS             7495..8214
                     /locus_tag="VFV99_30130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063689.1"
                     /translation="MRGWSVLLLVCGCSFEHGISVDTRDADADASASALADAAPLPCS
                     TRFVWTADFTVDPTTLNNNAGPEPDWRLRDGGALPGALADGVWAIADSPPVALETQPK
                     TDFNRLTRATARLRNTSPAGSRGVVFGLNADYSPMTYMPLYLEVRLAADTLTQTATLY
                     GRDTTGEITLASFPDLATGLLDVMLDIDPAANQVQVRVGSASSTYLYMPIPREMNDDR
                     FATIAAYSDSEVDEVRVEVCM"
     gene            complement(8301..9113)
                     /locus_tag="VFV99_30135"
     CDS             complement(8301..9113)
                     /locus_tag="VFV99_30135"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063690.1"
                     /translation="MSQGSPLAVPMPWDLVSAAYSEEVRPTFEIFARDALRMIAPRRE
                     SRIVDVACGPGTLSVLAAQDGHTVDALDFSPEMINHLKPRIGPLPITAHLGDGQALPF
                     ADGSFDAGFSMFGLMFFPDRAKGFAELRRVLKPGGKAAVSSWLAMDKIPVFAAMMGAV
                     RETMWKAMGSPPPPDRPSPLTTAEECHDEMSASFANVEVHQMTTIQRERSADALWESM
                     TRTMAPVVLMKKSLGDKFAAVDAAARTAIRGVVGSGAVEVTLRAHLTVGTAR"
     gene            9185..9889
                     /gene="rph"
                     /locus_tag="VFV99_30140"
     CDS             9185..9889
                     /gene="rph"
                     /locus_tag="VFV99_30140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5063691.1"
                     /translation="MRPDGRRPDQLRPLEIITGFQKHAEGSALIKLGDTWVLCAASLE
                     QGVPPFLVGKNQGWLTAEYAMLPRSTHTRSKRDPGGRGKEIQRLIGRSLRAAVDLSAL
                     GERTINVDCDVLSADGGTRVSSVTGAWVACALAIKKLVAAGQLPDMRALKPPVAAVSV
                     GVVNGQVVLDLPYVEDSKADVDMNVVGDESGKLIEVQGTAEHNTFDRKQLDAMLDLAI
                     AGIKQLAAAQRQAVGL"
     gene            9886..10521
                     /gene="rdgB"
                     /locus_tag="VFV99_30145"
     CDS             9886..10521
                     /gene="rdgB"
                     /locus_tag="VFV99_30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5063692.1"
                     /translation="MTRPLVFATRNKGKLVELRDLLPGIDVLSVDEAAQRLGREIPDV
                     VEDADTFAGNASKKAREVSALTGLPALADDSGLEVDALGGAPGVYSARYAGDQHDDAA
                     NNNKLLAALAGVPPAKRTARFRAVLALADVNGPLGGEVLTADGTCEGVILDAPRGTGG
                     FGYDPLFFAPELGQTFAEAGVGPKSDLSHRARAMKAMKQRLLAYLQQAGQQ"
     gene            10573..11445
                     /locus_tag="VFV99_30150"
     CDS             10573..11445
                     /locus_tag="VFV99_30150"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HEX5063693.1"
                     /translation="MRHTASATIVLTSVLAVAATVGAQTRDDSAAVPARTKQRSTQKA
                     EQPPAIDPRAIDALRKMSGFLRTQQNYAVDTTGKQDYVLPSGEKVQLSSHGNLIVANP
                     NRLRASVVSDRKERQFFFDGKTFTMFSPRPNYYTSITLPTRTTNRELVEHLDANYAVE
                     LPLADVLRWSTVGASINDITRATYIGTEWINNAETDHYSFRQRGLDWQVWIERGNQPL
                     PRRLVLTTTDDPARPEYAVDMTWHLGTRPSDAVFAFTPPNDAMKIAMASREQLASREQ
                     ANKARRTSQPTKED"
     gene            11456..12013
                     /locus_tag="VFV99_30155"
     CDS             11456..12013
                     /locus_tag="VFV99_30155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063694.1"
                     /translation="MNTLKSTLLTLTVALIASGTHAGGADARGFGGGFHGGGFGGFHG
                     ARTSIGGFHAGAFRAGGVHGTYARGAGGFHANVNRNYDVNRNWNAGDYHGDYGYYHGG
                     HYYAGYGYHGYYGGYGYPVGGAVAAGVAAGATAAAIGSIAYSLPAGCGSVYVGGLGYY
                     SCGGVYYRPEYQGSQIAYVVVNNPG"
     gene            complement(12036..12443)
                     /locus_tag="VFV99_30160"
     CDS             complement(12036..12443)
                     /locus_tag="VFV99_30160"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5063695.1"
                     /translation="MSTRTENKRAVIELFARVSANDIDGAMALVADDVSWRVPGKPEL
                     LAMAGLYDKKRLRYLFDVMRSQLQQGLAMTVVHAIAEDDCVAAEVEGSGELKNGRSYR
                     QQYHFMIRVRDGKIATVHEYFDTLHAHDIWIRR"
     gene            complement(12488..12898)
                     /locus_tag="VFV99_30165"
     CDS             complement(12488..12898)
                     /locus_tag="VFV99_30165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063696.1"
                     /translation="MSSLRTAIDDADRQNRGRTRRIGGLAFNALQAAIHDLRDELVAC
                     AKAADPSVRASRYRMHVVIDGHISVGAVVEDVSVKRLVTSGPDQPPTEVATSAQACIE
                     RLLMSIELPRSDGPGDFETVLETDRRCVDSAAHR"
     gene            13231..13932
                     /locus_tag="VFV99_30170"
     CDS             13231..13932
                     /locus_tag="VFV99_30170"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5063697.1"
                     /translation="MKKQSGQCELDLRRRTWGGARAGAGRTKKPSKKDPAHRTRPVLR
                     AYHPVHVVMRVKKGVGRLRKGPAYRAIRRCLVKCLGNEAFRVCHISIQANHLHFLVEA
                     KDSSSLASGMQRLNILTARALNRELGRHGKLFEFRYHATQIKSPKQARHALAYVLNNW
                     RRHDEDESCERAQLATLDPYASGLSFDGWDSPRFAKPVDFTPLPVSEPRTWLLGVGWK
                     KHGLIDPHEVPGPLR"
     gene            14180..14725
                     /locus_tag="VFV99_30175"
     CDS             14180..14725
                     /locus_tag="VFV99_30175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063698.1"
                     /translation="MRVYKPMIVDGEQRFVANVIAIAKGLIAFGELLPERGMAPKFGT
                     PIHSLLIHLGDDQVLFELNDQGNTRYASNVERVELTPTTLRIVFVAGQGPYSGRVSLS
                     PRIEVFDRDTHQPQKLRSVLVEMKPTATQLARIRQRLGDHKLIRRTSASASASVARKK
                     SAPKASGARHRVQRSKRRPTR"
     gene            complement(14776..15303)
                     /locus_tag="VFV99_30180"
     CDS             complement(14776..15303)
                     /locus_tag="VFV99_30180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063699.1"
                     /translation="MSIDFNALFGIGSSYCGADWLRGALAAEPPGVREAVARFRHLWT
                     PEEWTAGERDGSTSIVGPGGFSLDIYTSTASLYHVIPWARFTRKDDERGILRRACFEI
                     ASLLDLPRALYLHELLPIGPVNNLDLQELETQLRASFGAPSSTFEALANAGEFSAGCW
                     YVDDFADLNTASSSR"
BASE COUNT         2724 a         5189 c         5036 g         2581 t
ORIGIN      
        1 ggctggacgc gggtgcgatc gtgtttccgc aaccggcgag cgagtgggcg atcgagaacg
       61 cgggcgcgct gatcgaatcc ggctgcgcga tcgtgcggct gcgcaacgtc gtcgaggtgc
      121 cattcgccgg ccttgcagtc gccgcacgtg atcgctactt cgcgcaggac gcacgcgatc
      181 ggctgcgctc gctgatcgaa gcgcactgga cgcgcacgat ttccaacacc gacttcgcgc
      241 gctgggcgca gacgttgatc gcgcgcgtcg tcggcagcga gcaagcgcgt agctcgatcg
      301 tgatgccgcc cggtggcgcg acaccgatcg gcacgctcgg cgcgatgtcc gctacattcg
      361 agctcgccga ccaagtcgcg gccggtatcg caccaccgcc gcggcgcatc gtgcttcccg
      421 tcggcagtac gtgcacgacg gctggtttga tctcgggctt cgcactcgcc cacgcgattg
      481 gcgtgtggcg ctggccggtg ccgatcgtcc acgccgtgcg cgtgacgccg tggccggtga
      541 cgtcgcgcgt gatgatcacg cgactcgccg cattcacgct cgcacgcatc gcgcagctcg
      601 gtggtccgcg cgtcgagctc ggcgacgctc gcctcgtgat cgatgcgcgc gagctcggcg
      661 ccggctacgg ccgaatcacg aagcgcggca cgcaggcgat gtcgcggttc gcacacgaca
      721 gcgcgccacg cctcgacggt gtgtactcgg caaaagcggc cgcggcgctg ttgcgtctgc
      781 atcgcgaagg cgtcggtccg ctcgtgttct ggtcgacgaa atcgacgacg cagctcgcgc
      841 cgccgtcacg gcccgcgatc gcacgagcac cgcgcgcact cgcgcgttgg ttcgcgagct
      901 gactgcgagt aaacgcgctg cccgcgaggg cctagtgtgc ggcttcggca ggctggtcgg
      961 cgttgacacg cttctgcgag cgcgccttgc cgctgcgctc ggcgtcggcg tcgcgctcgg
     1021 ccgcgatgta cgcctggcac ttcttgagcg cgcggtcggt cgcgacgtat tgcgaataga
     1081 actcgggcgc gcgattgctg atcagcacgg cgaccttggc cgcacccgca caatcgccct
     1141 tgcctgcgag ggactgcgcc tgactgtgct ggcccttcgc ccacgcgatc agcgcggagt
     1201 ccttgcccgc ggtcgagtcc ggcttcgatt cggtcttctc ggcttccttc gccaccttcg
     1261 caggaggcgt cgtcggcttt gccgtcgccg tcggcgactt cggttgctgc tgcgcgacct
     1321 gcgccggagc cggagccgtc gttgctgcag gaggaggagg aggcgcaggc gtcggcgcgt
     1381 tgcgcgcagg tcccgcggcc gcaggcggcg tcgtcgcgac gcgcccgcct ggcgcacccg
     1441 taccggttgc accaccgacg gtgccaccgc caccggcgcc agcgcgcgcg accgtcgagc
     1501 ccgtcgcggc ggtgtcaccg cgaccgcgat tgctgtcgaa gctgtcggtc gcaatctcgg
     1561 cgtcctcgcc gccctggacg ttgcccttct tcgctccgac cttcgaggca accttcgcgc
     1621 gcggctcgtc gagctctttc ggctcgcgtt gcggcgtgtc gacgatgatc gagctcgacg
     1681 cggcacgctt gttcttcgcg accgcgagct cttgctggcg ctcgagcgcg ttctgctgat
     1741 ctttgagctt cgcgacttgc tcggcgcgat cgctcgcttg ctgcttcgct tcagctttgc
     1801 cgtcggcgag ctggacctga tagccgaagc tcgagccgga gccttgcgcg atgccggtgt
     1861 cggcggcgcc aacaccttcg ttcgcggcgg cgggatccat cgcgggcgct tcgaccgcta
     1921 ccgtgtccgc cttttcctgc agcgctggcg gctcggcttg cttgtcgacg aactggtcgc
     1981 tgcccttctt catgtagagc gtgccggcga cgccgacgac gagcacgagc atcgctgccg
     2041 ctgccatcgc cggatgcgcg aggaacatgc gcgagaaccg gtagaaccag ccctcgttcc
     2101 cttcatgctc acgctcggga gcgcgcttcg gagcgcgtcg gtgcgcttct tggagcagca
     2161 gcgccgacac cgcctgcggt ggatcgacct ggagctcgaa gatgcggctt tcacgtaccg
     2221 cgttgcgggc gctcttgagg tcgtcgaggg cgccccggtc ggtcgggtgc gagtcgagat
     2281 gggccgccag gcgggcctcg tcggcgggcg ttagctcgcc gtagagggcg ccgatcagca
     2341 gcgcatcaat gtcctgacga tccatgacct tgtgtcctta tactacgtgt aaccaaaccg
     2401 aacgacgagc gaggctttcc gagcacttgg ctgttactac gtgccgtctc ggaccaccgt
     2461 atcgtcgctt gctgattaca aacgtaggag gagcgccatg gatctaacgt ggtgctgtaa
     2521 atgcactacg ccgcaggtcg caggggcaac ctggcggatt cgctctagtc cttcgcaggc
     2581 tcgcaacccc actgcacgga ctcgatcgtc catgcctcat cgccacagac cgcggcagac
     2641 gcctcagcgg tttgctcacc gccttcgagc aacgtgaact tctgagagct cgcgtgcacc
     2701 gcgcgacgct tctgcgagtc aggcgagcag atcaccttcc acgagatcga gcagtcgacc
     2761 tgcgccttgc agctgttgtg aacgttcatc tcgaacgcgt cgtcgccctt ctccttctgc
     2821 gtgaaggacg tgcagtccga tagcgacttc ttcgcgtgcg agcctttggc gagcacaggc
     2881 atagcgatca tcatggcgag ggcacaagct gcccatcgcg cccagcgggt gcgcatacga
     2941 cctccgaatg caggtatccc tgcgggtctt ccggcgcgct ctttgagagt cgcagcgccg
     3001 tcgtcgaagc aatagacaac gaggcccgcg tgcggtttcg aaaatctcgc agcgcgtgca
     3061 agatccgcga atgcaaagcc ctctcgacgt cttccgcccc ggcgttctcg acggtcacgt
     3121 ggcgctcgtc accggcggtg gtaccggcat ctgtcgtggc atcgccgccg cgtatgcgcg
     3181 cttcggcgca gacgtgtgca tcgtcagccg caaacaagac gtgctcgaaa agaccgccgc
     3241 cgagctatcg gcagcgagcg gccgccaggt actcgcggtt gccgccgacg tgcgtcagcc
     3301 cgacgacgtc gcgcgcgcgg tcgcgcagac cgtcgagcgg ttcggcaagc tcgacacgct
     3361 cgtcaacggc gcggccggca actttctcgc gcctgcagcc ggactgtcgc ccaatggctt
     3421 tcgcaccgtg atcgacatcg atctcgtcgg cacgtttcac gcggcgcgcg ctgcattcga
     3481 gcaactgcgc gcgagcggtc gcggcctcgt gctcaacatc agcgcgacgc tgcactatca
     3541 cggcacgccg ctgcagattc acgcctcggc ggcgaaagcc ggcgtcgatg cgatcacgaa
     3601 gaacctcgcc gtcgagtggg ggcagttcgg cattcgctcg tgcggcatcg cgcccggacc
     3661 gatcggcgac accgaaggca tgaaacgcct cgcgcctggc gacatcgcga agaaagcgat
     3721 ggccggcatt ccggcgggac gcttcggcac gatcgacgag atcgcggccg ccgccgtcta
     3781 tctgcgctca cctgccgccg cgtacgtcag tggtcacgtg ctggtcgtcg atggcggtca
     3841 ctgcgtttcg acaccctcgc tgatgggagt ttgatgatcg atctcgtcgc gcccgcacgt
     3901 ccggttgcca tggaactcgt gtcgggacgc ggccattacg atcgcgtgct cgcggccgtc
     3961 ctcggcgcga aggtcagcgt gtggatcgcg accgcgaacg tcaaagagct aatggtcgaa
     4021 gaccatcgcg caaggccggg ccgtcgccgc tcgctgcgca aagcgaatta cgtcagcgtg
     4081 ctgtcgcatc tcgacgagct tgccgcgcgt ggcgtcgagc tgcgcttgct ccatgccgag
     4141 ctgccgtcgc ggccgtttcg cgaagagctc gcgcagcatc cgaagctcgt cgccggcggg
     4201 ctcgcgctgc gccgttgtcc gcgcgtgcac ttgaagacgg tgatcgtcga tggcgagctg
     4261 ctctatctcg gcagcgcgaa ctggaccggc gccgggctcg gcgcgaaggg cagcggtcgg
     4321 cgaaacttcg agctcggcat cgtcaccgac gacgcgcagc tgctcgacca catccaggcg
     4381 ctgtacgagc aaatttggac tggcggcgag tgcgcgacgt gcaagcttca agacgagtgt
     4441 ccgggtccac tcgtccagct cggtcgtgcg ccggcaacgc cgccagtgcc gaccaaacgc
     4501 gtccgcaaaa cacgcaagaa accgcggcgt tgagcccacc tgtaaccggc agccctgccc
     4561 ggtgcacgat atagctggtg accgtggctg cggcgtacgt ggaagacgtc ctgctcgttg
     4621 accggtgttt gaccggcgag ccggcagcga cgcgtgagct gtttcggcgc caccgtggcc
     4681 gcgtgcacgc gagcttgttc cgcgtgctcg gcagcaatcg cgacatggac gatctactgc
     4741 aagaggcttt catccaggtg ttccagtcgc tgcgcggctg gcgcgccgaa gcgagcctgt
     4801 cgacgtggat cgatcgcgtg tcggtacgcg tcgcgtaccg ctatctgtcg cagcgcggcc
     4861 gccgcatcca gaccgatccg atgatcgatg acgacaacgt gcccggcggt gaagagccgg
     4921 gcgcacggcg gcagctcgcc cgcgacggca tcaagcgtct ctacacagtg ctcgacgagc
     4981 tcggtgcggc gtcgcgcctc gcgttctcgc tgcacgagat cgatggccgt ccgctcgctg
     5041 aggtcgcgga gctcgtcggc tcgtcggtga ccgcgacgaa gttgcgcgtg tggcgtgcac
     5101 gcaagcgcgt gctcgaagcc gcggctagcg atccgatcct cagcgaattc atcgagcctg
     5161 gtttcctcgc caaggagagc gcatgaacct cgtcggcaaa gtacccgtcg agccgctcga
     5221 cgaggaacga ctcaccaaca tcgagcgcaa gctcgtggtg caggtctccg aaatgtcgca
     5281 gcggccgcat cgcgcgccgc gccgcggatt cgcgtacgcc gggtttgcga tggccgtggc
     5341 aattgcgggc ttcgtcggct ggaagctgca ccgcgatccg gtcgtcgagc ccgcgaagcc
     5401 cgagcagctc gcgatgaagg ccggcgcgct cgatctcggc gacgcgcaga ttaccggcag
     5461 cgacttcgac atcacgcgca ccgcagcgct cacgatggtc gtgatgcacc cgggcaagct
     5521 cgatctgcac gtcgagcaca agccgggtcg tgtgttcgtc gtacaagcgg gcgacgtcga
     5581 gatcgaggac atcggcacgc ggttcactgt cgattacgac ggcaagaacg tcgacgttcg
     5641 cgtcaccgag ggtgaagtga aggtgaagcg cgcgggcaag gagctgcgcg tcaccgcggg
     5701 caacgcgtgg acgctcgaga tcgggccgat cacgatcgcg cagctcacgg cgcagcaagc
     5761 gcagaccgtc ggcgcgacga atccgcagcc gcaagcgacg gcgcaaaaca atgttgtcgc
     5821 catggcgccg actaacaatg cgggcagcgg aagtggtgtc ggtagtgccc agggaagtgg
     5881 cgcctcgcga ccggcgaaga cgccgcagtc caacgcgaag agagcgatcg acaacctccc
     5941 gctggagccg ctcgtcgcga ccgacctcaa ggatccgaaa gcggcaattg ccgagtatct
     6001 gaagacggtc cgggacatgc ccgaaggcga ggacaaagcc gagctcctcc agagcatcgc
     6061 ggtcatgcag ctgcgccaga aggacctcga tggggccgcg cactcgatca agggcatcct
     6121 ccagcgccaa ggtggcgata cccccgcgta taagcaggcg ctctggctcg acatgcggat
     6181 caagtgcgcg acgtcgtacg accgcgataa caaccgcgac ggcaaggccg accgcctgtt
     6241 cgacgacgat tgccgccgct cggccgagcg ctacatcgtc aagttcccga ccggcgccgc
     6301 tgcaggagtt gcgcaggaag tactgaacga aatctcgcgc ggacagtgac gttcactttc
     6361 gggaagcact acggaaacag cgccgacaat ttcgcgcgtg gggtagctcg ctgatttgat
     6421 cgcggcagta gcagtacact gccgccgtgt cgcggcgtac ggtggtcctc gagctcgcgg
     6481 gcgaacatca gctcgctcga gatctcgtgg ccggcggcgt gttcgtgccg ggagaagttc
     6541 tcgcgatcgg cgaggactgc gacctcctgt tgcgcggcga agagggcgag ctcgcgctgc
     6601 acgcgacgtg cgtgtgggtc gatcgcgaga aaggctcggg cttgcaactg atgggctgcg
     6661 acgccgagat gaagcaacag atcgcgacgc tcgcagtcgg cacgcagctc gaagacatcg
     6721 cgacgtccga agtcgagagg gtcgatatca cgctcgacga catgacggac gacgacaccg
     6781 aggagtcgca atacgaggcc gacggtccgt ccgccgaaga gctggcagct gccgagctcg
     6841 aagccgagct cgcgggcgac gatgaagaag atgccgagcg caagctcgcg ctcaacgtca
     6901 acgagcgact gcgcgggctc acgctcgtcc agcagctccg cgtcgcacag cgcggcgagc
     6961 tctcggagcg gatcgtcctc gagcgcatgt acggcaagaa cgtgtgggac gcgctgttgc
     7021 gcaaccagcg actcaccggc cccgaggtcg cgcgcatcgc ccgcatgggc gcactgcccc
     7081 gcccgctcat cgagctcatc gtcggcaacg gcgcgtggct gcagattccg gaagtccggc
     7141 gcgcgctctt gtcgaacccg cggctcgcaa ccgatcagat ttcgcgcgtc ttgcgcctgt
     7201 tgccgaaaca cgagctcaag ctcgccagtg ttcagacggc gtatccattc gccgtgcgcg
     7261 atgcggccaa gcgcatgctg cgcgagatga gcggctaatc agctgccgct gcccatgtga
     7321 cgtagcaagc aggctctccc gcccggcctg tgatgcaaac gcatcgtcaa tcacgattcc
     7381 ttcatcgcgt gtcaccaact ggtgctcgca ggcacgcgcg agcacgccga atcacgtcac
     7441 ggcatgttgg cgtctcgtcg ctcgagcacg cgtcgcgccg acgacaatgg agctatgcga
     7501 gggtggagcg tcctgcttct tgtttgcggc tgttcgttcg agcacggcat ctcggttgac
     7561 acgcgagacg cggacgcgga cgcgagcgcg tccgcgctcg ccgatgcagc gccgctgcca
     7621 tgcagcacgc gcttcgtctg gacggcggac ttcaccgtcg atccgacgac gctcaataac
     7681 aatgccggtc ctgaaccaga ctggcggttg cgcgacgggg gcgcgttacc aggagcgctt
     7741 gccgacggcg tgtgggcgat cgccgacagc ccgccagttg cgctggagac ccagccgaag
     7801 actgacttca atcgactcac gcgtgcgacg gcgcgcctgc ggaatacatc gccggctggt
     7861 tcgcgtggcg tcgtcttcgg tctcaacgcc gactactcgc cgatgacgta catgccgctc
     7921 tacctcgagg ttcggctcgc cgccgacaca ctgacgcaga ccgcaacgct ctacggcaga
     7981 gacactaccg gcgagatcac gctcgcgtcg tttccggatc tcgcaaccgg cttgctcgac
     8041 gtgatgctcg acatcgatcc cgcggcgaat caggtccagg tccgcgtcgg cagcgcgagc
     8101 tcgacgtatc tctacatgcc catcccacgc gagatgaacg acgaccggtt cgccacgatc
     8161 gccgcctaca gcgacagcga ggtcgacgag gtgcgcgtcg aagtctgcat gtgaagctgg
     8221 ccggcagcgg ccgtcatgac gaagttgatc gacgtatcga tccggcagtg ggactcggtc
     8281 acgtggcgct cacagctgac tcaacgcgca gtaccgaccg tgaggtgcgc gcgcagtgtc
     8341 acctcgacgg caccgctgcc gaccacgccg cggatcgcgg tacgcgcggc ggcatcgacg
     8401 gcggcaaact tgtcgccgag tgacttcttc atcaacacga caggcgccat cgtgcgcgtc
     8461 atcgactccc acagggcgtc ggccgaccgt tcgcgctgga tggtcgtcat ctggtggact
     8521 tcgacgttgg cgaacgacgc cgacatctcg tcgtggcact cctcggcggt ggtgagcggg
     8581 ctcggccggt cgggcggcgg cggcgagccc atcgctttcc acatcgtctc gcggaccgcg
     8641 cccatcatcg cggcgaatac tgggatctta tccatcgcga gccagctcga cacagccgcc
     8701 ttgccgccgg gcttcagcac gcgacgcagc tcggcaaacc cctttgcgcg atcgggaaag
     8761 aacatcagtc cgaacatcga gaagcccgcg tcgaacgagc cgtcggcaaa cggtagcgct
     8821 tggccgtcgc cgaggtgcgc agtgatcgga agcgggccga tgcgcggctt caggtgattg
     8881 atcatctccg gcgagaagtc gagcgcgtcg acggtgtggc cgtcctgcgc cgcaagcacc
     8941 gacaacgtgc ccggaccaca agcgacgtcg acaatgcgcg actcgcgccg tggcgcgatc
     9001 atgcgcaacg cgtcgcgcgc gaatatctcg aatgtgggcc tgacctcctc gctgtacgcg
     9061 gcggagacga gatcccacgg catcggcact gcgagcggcg agccttgcga catgcggcaa
     9121 cgttacagca aacgcatgcc ctagaccttc gctccagtgc cgatcgcggg gtagcctccg
     9181 cgcgatgcga cctgatggaa gacgtcccga tcaactgcgc ccgctcgaga tcatcacggg
     9241 ctttcagaaa catgccgaag gctccgcgct gatcaagctc ggcgacacct gggtgctatg
     9301 cgccgcgtcc ctcgagcaag gcgtgccgcc attcctcgtc ggcaagaacc agggctggct
     9361 cacggccgaa tacgcgatgc tgccgcgctc gacgcacaca cgcagcaagc gcgacccagg
     9421 cggccgcggc aaagaaatcc agcgcctgat cggtcgctcg ctccgggccg ctgtcgacct
     9481 gtccgcgctc ggcgaacgca cgatcaacgt cgactgcgac gtcctgtccg ccgacggcgg
     9541 cacccgcgtc tcgtcggtga ccggcgcctg ggtcgcctgc gcactcgcga tcaagaagct
     9601 cgttgccgcc ggccagctgc ccgacatgcg cgcgctcaag ccgcccgtcg ccgcggtctc
     9661 ggtcggcgtc gtcaacggtc aggtcgtgct cgacctgccg tacgtcgagg actccaaagc
     9721 agacgtcgac atgaacgtcg tcggtgacga gtcgggcaag ctgatcgaag tccagggcac
     9781 cgccgagcac aacacgttcg atcgcaagca actcgacgcg atgctcgacc tcgcgatcgc
     9841 aggcatcaaa cagctcgccg ccgcgcaacg tcaggcggtc ggcctgtgac gaggccgctc
     9901 gtcttcgcga cgcgcaacaa gggcaagctc gtcgagctac gcgacctgtt gcccggcatc
     9961 gatgtgctgt ccgtcgacga agccgcgcaa cgactcggcc gcgagatccc agacgtcgtc
    10021 gaggacgccg acacgttcgc cggtaatgcg agcaagaaag cgcgcgaggt ctccgcgctc
    10081 actggcctgc ccgcgctcgc cgacgacagc ggcctcgaag tcgacgcgct cggcggcgcc
    10141 cccggcgtct acagcgcacg ctacgctggc gatcagcacg acgacgccgc gaacaacaac
    10201 aagctgctcg ccgcactcgc cggtgttcca cctgccaagc gcaccgcgcg ctttcgcgcc
    10261 gtgctcgcac tcgcagacgt gaacggcccg ctcggcggcg aggtgctcac tgccgatggc
    10321 acgtgcgaag gcgtgatcct cgatgcgcca cgcggcacgg gcggcttcgg ctacgatccg
    10381 ctgttcttcg cgccggagct cggccaaacg ttcgccgagg cgggcgtcgg tcccaagagc
    10441 gacctctcgc atcgcgcgcg agcgatgaag gcgatgaagc agcgcctgct cgcgtatctg
    10501 cagcaggcgg gtcagcaata gcgaaacacg tcgcgcacgc ggtgtgcagt tggaagcctg
    10561 cggagcgttc caatgagaca cacggcaagc gcaacgatcg tactgacatc cgtcctcgca
    10621 gtcgccgcga cggtcggcgc gcaaacgcgc gacgactctg ctgcagtacc ggcaaggacg
    10681 aagcagagga gcacgcagaa agcagaacag ccacctgcga tcgatccgcg agcaatcgat
    10741 gcgctacgca agatgagcgg gttcctgcgc acgcagcaga actacgcagt cgacacgacg
    10801 gggaagcaag actacgtgct cccctccggc gagaaagttc agctgtcttc gcacggcaat
    10861 ctcatcgtgg cgaatccgaa ccgcctgcgc gccagtgtcg tgtcggaccg caaagagcgc
    10921 cagttcttct tcgacggcaa gacgttcacg atgttcagcc cgcggcccaa ctactacacg
    10981 agcatcacgc ttccaacgcg cacgaccaat cgcgagctcg tcgagcacct cgacgcgaac
    11041 tacgcggtcg agcttccatt ggccgatgtc ctgcgctgga gcacagtcgg cgcaagcatc
    11101 aacgacatca cgcgcgcgac gtacatcggt acggaatgga tcaacaacgc cgagaccgat
    11161 cactactcgt ttcgtcagcg tggtctcgac tggcaggtct ggatcgaacg cggcaaccag
    11221 cctctgccac gcagactcgt gctgacgaca actgatgatc cggcgcgtcc cgagtatgcg
    11281 gtcgacatga catggcacct cggcaccagg cctagcgacg cggtgttcgc attcacgccg
    11341 ccgaacgacg cgatgaagat cgcgatggcg tcacgggaac aactcgcgtc gagggagcaa
    11401 gcaaacaaag cgcggcgcac ttcgcagccg acgaaggagg actgacatgc agtccatgaa
    11461 cactctcaag tccaccttgc tgacgctcac cgtcgcgctg atcgccagcg gcactcacgc
    11521 cggcggcgca gatgcgcgag gcttcggtgg cgggttccac ggcggcggct tcggcggctt
    11581 ccatggtgcg cgcacgagta tcggcggttt ccacgccgga gcgttcaggg ccggcggagt
    11641 ccacggcacc tacgcgcgcg gcgccggcgg cttccacgcc aacgtgaacc gcaactacga
    11701 cgtgaaccgc aactggaacg ccggcgacta ccacggcgac tacggctact accatggtgg
    11761 ccactactac gcgggctacg gctaccacgg ctactacggc ggctacggtt accccgttgg
    11821 tggcgcggtg gcggcaggtg tcgccgcggg cgcgaccgcc gcagcgatcg gctcgatcgc
    11881 gtactcgttg cctgcaggct gcggcagcgt ctacgtcggc ggcttgggct actacagctg
    11941 cggcggcgtc tactaccgac ccgagtacca gggcagccag atcgcctacg tcgtcgtgaa
    12001 caacccgggc taggcggaaa tcagccgcgc acccatcagc ggcgaatcca gatgtcgtgc
    12061 gcgtgcaacg tgtcgaagta ctcgtgcacg gtcgcgatct tgccgtcgcg cacgcggatc
    12121 atgaagtgat actgctggcg gtacgatcgc ccgtttttca gctctccgga accctcgacc
    12181 tcagcggcga cgcagtcgtc ctcagcgatc gcgtgcacca cggtcatcgc aaggccctgc
    12241 tgcagctgcg acctcatgac atcgaagagg tagcgcaagc gtttcttgtc gtagaggccg
    12301 gccatcgcga ggagctcggg cttacctgga acgcgccagc tcacgtcatc tgcgacaagc
    12361 gccatcgcgc cgtcgatgtc gttggcgctc acacgggcaa acagctcgat cacggcacgc
    12421 ttgttctcgg tccgagtaga catggcgcgg cagcataacg tcgatggcgc gtgtgttcgt
    12481 cgctagctca gcgatgcgct gcggagtcga cgcaacggcg atcagtttct agaaccgtct
    12541 cgaaatcgcc tggtccgtca cttcggggga gctcgatgct catcaagagc ctttcgatgc
    12601 aggcctgcgc tgaggtcgcg acctccgttg gcggctgatc cggacctgag gtgaccagcc
    12661 tcttgacgct gacgtcctcg acgacggctc ccaccgaaat gtggccatcg atgacgacat
    12721 gcatccggta gcgagacgcg cgcaccgaag ggtcggcggc ctttgcgcac gcaacgagct
    12781 cgtcgcgcag atcgtgaatc gccgcttgca gcgcgttgaa tgcaagcccc ccgatgcggc
    12841 gcgtgcgtcc cctgttctgc ctgtctgcat cgtcgattgc ggtacgcagc gagctcattc
    12901 ccgtctgatc gagtcgacgg tagccgccat cggcgggatc tcgagtcgct tctcgcgacc
    12961 gccggtcgtc gcacgcggag gccgatgcca cgcgtggttg tggctccgtt gctggttgtg
    13021 acggcacggt tcgtgtcgag cttgaccagc aagcaccaag gacgaccgcc atgacaaccg
    13081 tggacgtgcg cgctgacatc gctcctgtgc ccccgtcatt tccggccgcg tgctccagct
    13141 tcctcgagaa tcccgccatc gacatcgaga ccacgattct acctcgccac cgtcgcacgt
    13201 cagaccgatt tgatatatgc cctaatcaag atgaagaagc agagcggtca atgcgagctc
    13261 gatcttcgtc ggcgcacatg gggcggtgcg cgggcggggg ctgggcgcac gaagaaaccg
    13321 agcaagaagg atccggcaca ccgaacgcgc cctgtccttc gtgcctatca cccggtccat
    13381 gtcgtgatgc gcgtgaagaa gggcgtgggt cgtcttcgca agggtcccgc atatcgtgcg
    13441 atccgccgct gcctcgtgaa atgtctcggc aacgaagcgt ttcgcgtctg ccacatctcg
    13501 atccaggcga accacctgca tttcctcgtc gaggcgaagg acagctcgtc gctcgcaagc
    13561 ggcatgcagc gtctcaacat cctgacagct cgcgcgctca accgcgagct cggccggcac
    13621 ggcaagctgt tcgagttccg ctatcacgcg acgcagatca agagtccgaa gcaggcgcgt
    13681 cacgcgctcg cgtacgtgct gaacaactgg cgtcgccacg acgaagacga gagctgcgaa
    13741 cgcgctcagc tcgcgacgct cgatccgtac gcgagtggcc tgtcgttcga tggctgggac
    13801 tcgccgcggt ttgcgaagcc cgtcgacttc acaccgttgc ccgtctccga accgcggacg
    13861 tggttactcg gggtcggctg gaagaagcac ggactgatcg atccgcacga ggtgccgggg
    13921 cctctgcgct gaggtcgatc gggctctcgc gcacggcgct ctaccgcgag aagttgcgtt
    13981 tgcccgcgct ttcgatgcac cgacgcgcca agacatcatg ctcgcgtacg agcaggacga
    14041 cacgcagtgg cgtgatgtca ccgagctggc ccggctgcgc ccgcacatgc ccctcgacga
    14101 agtgatcacg tagctgcagc accaagccgc ggaagtggct ggttgtcacg cgagatgagt
    14161 gcgtctaacg tgcgcgcgag tgcgggtcta caaaccgatg atcgtcgacg gcgagcagcg
    14221 attcgtcgca aatgtcatcg cgatcgcgaa gggactgatc gcatttggcg agctgctgcc
    14281 ggagcgcggc atggcgccca agttcggcac accaattcac tcgctgctga tccatctagg
    14341 tgatgaccag gtgctgttcg agctgaacga ccaaggcaac acgcgctacg cgagtaatgt
    14401 cgaacgcgtc gagctcacac cgacgacgtt gcggatcgtg ttcgtggccg gccagggtcc
    14461 gtactccggg cgcgtgtcgc tgtccccacg gatcgaagtc tttgatcgcg acacgcacca
    14521 gccgcagaaa ctgcgcagcg tgcttgtcga gatgaagcca acggccacgc agctagcgcg
    14581 gatcaggcag cgacttggcg accacaagct catcaggcgc acctcggctt cggcttcggc
    14641 ttcggtggct cgcaagaagt cagcgccaaa ggcgtcgggc gcgcgtcacc gcgttcaacg
    14701 gtcgaaacga cgcccgactc gctgagcgcg gtcgcagccg ctgtgcggtc caccggacgg
    14761 agcgtagtga actattcagc gcgaggagct cgcggtgttc aggtccgcaa agtcgtcgac
    14821 ataccagcag ccggcgctga actcgcctgc atttgctaag gcttcgaacg tagaggacgg
    14881 cgccccaaat gaagctcgca gctgtgtctc gagctcttga aggtcgaggt tgttgaccgg
    14941 cccgataggc aacagctcgt gcaaatagag ggcgcgcggc aaatcgagca gcgacgcgat
    15001 ctcgaagcag gcgcgtcgga ggattccgcg ctcatcatct ttccgtgtga accgcgccca
    15061 cgggatcacg tgatacagcg atgccgtgct cgtatagatg tccaaggaaa atccccccgg
    15121 gccgacgata ctcgtacttc cgtcgcgttc gccggctgtc cattcctccg gggtccatag
    15181 gtgccggaag cgcgctaccg cctcccggac tccaggcggc tcggctgcga gcgcaccgcg
    15241 tagccagtca gcgccgcagt agctgctccc aattccgaac agggcgttga agtcgatgct
    15301 catgacggcc tagagagcgt cttacatggc aacagcacat cgcgatgatc aggcgcccag
    15361 cacaaacgtt tgcgctgcag tcgccgtggc caatcagtgc gctgcggctg ggaagcggcg
    15421 taacaccgcg ccttaagagg tgttgcgaac ggacccgccg ctgcaacagc agcgggtcac
    15481 tacggggcgg acgacggggc tcgaacccgc gacatccagg accacaacct
//
LOCUS       DASUFX010000747        10679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1649948, whole genome shotgun sequence.
ACCESSION   DASUFX010000747 DASUFX010000000
VERSION     DASUFX010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649948"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1550)
                     /locus_tag="VFV99_30185"
     CDS             complement(141..1550)
                     /locus_tag="VFV99_30185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5063700.1"
                     /translation="MVAALFVLVTASGADAALTAVSNFGSNPGALNMYEYVPPNLPTG
                     RPLVVVLHGCTQTAANMENAGWNKLADQYQFAVLYPEQTSANNPVKCFNWAGEYGDTA
                     NLMRGMGENQSIISMIDYMHTTHGTDNAKTFIMGFSAGGAFVPVMLATWPERFAAAAV
                     MEGIAYKCATSVNGAYSCQSPGVTHTPQEWGDLVRGAYTFSGTRPRIQIWHGSSDTTV
                     VPANQTELQKQWTNVFGTDMTADETEMIGSAATRTAYKMGATIVVESYTVNGMSHAVA
                     VGNEGSTVCPATAGSYFEAKGICATLRAAKFFGLTGGGGGSGSGSGSGGTGAAPTIEI
                     VSPADGDQVTGMMVVVVAAGDDVAVTDVELKIDGTSVGMDMEAPYQFDWDASAAGAGM
                     HELEASATDGDGNVTTVKAMVKVPGPGGSGGGGDDDGSGPGDLPACSLNAGKSDGRAW
                     APIVFAVVIVVGYSRRRRK"
     gene            1711..3027
                     /locus_tag="VFV99_30190"
     CDS             1711..3027
                     /locus_tag="VFV99_30190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063701.1"
                     /translation="MVDTPRTKLQPRPVRLVHKLRVTVNAGVDAGATCAPDDGSRLAI
                     GTSEDNALVLTDAAVSRYHLELLRTPAGVEVIDLGSRNGTWVGGVRVSRAIVPAGTQL
                     RLGDTTLTVEDAGSTVAPPPPSDVPRSTDLVGESDAIREVARLVHRLARVDSSVLIQG
                     ETGTGKEVIARAIHEASPRRDHELVVVDCGSMPASLIASILFGHEKGAFTGADQRRAG
                     AFERAAGGTVLLDEIGELPLDVQPALLGVLERRAFTRVGGAQPIAVDVRVLAATHRDL
                     RAEVNAGRFRADLYYRLAVAKVLIPPLRERPEDIEPLVAHFVQRLTGVAELGPLASAL
                     DQLRAHPWSGNVRELRNVVEAALVMGELDLGTGAAHTAQPIVATNGAAITPYRDARAT
                     ALARFEADYLRTLIERAEGNASEAARIARMDRPYLLTLLRKHGLRS"
     gene            3215..3556
                     /locus_tag="VFV99_30195"
     CDS             3215..3556
                     /locus_tag="VFV99_30195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063702.1"
                     /translation="MTKLIGLKRLVLIMIPAMTIAMAPACTTMLGSDTSAASKMGADD
                     SDTDSDSDDGDSDCSSVDEDSDTDGDTDEPGDTDSDNDSDSDSDCGGGGGSGSGSGSG
                     SGSGSGSGGPV"
     gene            3566..4762
                     /locus_tag="VFV99_30200"
     CDS             3566..4762
                     /locus_tag="VFV99_30200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063703.1"
                     /translation="MLATLALIALGASLVPAHRAARSSPALLTALITTEAIGLVLVGV
                     AVVSARTWSSIAQEDGVIEWATFLAYVFAAGWLLLVVRKQSPSWWFKGATFLLAAFCI
                     VVAGEEISWGQRLFGFKPPDVFLERNFQQEMNLHNVLMDERGLGFKLESKDLVVVVVL
                     GFCAVWPWLVRRRRFTVFGPLAPPYALLPLALGVAAAELAYNVELTGEGAELVAGLIF
                     LASALVVAQPPPRVVMTWLLAPLVVGLLLSTILARVMFGSDEDGTRTAAAELALLSQD
                     VANGATDKLLKKNVHKRAFTSMHDGYVTLAGGTFYEGQAKRHDRRGYCLDPWNNPYWI
                     FYDKSSRTGAVYSFGPNRRRDIVVRDPKSDPGDDVIVRFRIPETAAAAPPEQPAQPDD
                     PTPPQP"
     gene            complement(4759..5310)
                     /gene="moaB"
                     /locus_tag="VFV99_30205"
     CDS             complement(4759..5310)
                     /gene="moaB"
                     /locus_tag="VFV99_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX5063704.1"
                     /translation="MTIVQHSRRMEREFIPVNVAVLTVSDTRTEANDTSGKYIADRLS
                     QEGHRIVARTIVKDVESAIREQMRAWIEGGEVEVIIATGGTGVTPRDVTPEALAPLIT
                     KHIPGFGELFRQLSFAEIGTATIQSRADAALCGQTYVFLLPGSTGGVKLGVDKILVPQ
                     LDHRLKPCNFVMLLPRIKNEKKS"
     gene            5421..5795
                     /locus_tag="VFV99_30210"
     CDS             5421..5795
                     /locus_tag="VFV99_30210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063705.1"
                     /translation="MATKLEATTSPTFTRTDRTGFVPGVANLALDVADRGQSTVIAVL
                     QDARGELRTLVENGIELAEKTAAQAFRFARTLTKRVDDAAAETLNNTERLLGNAVRSA
                     RETAKSATDTANTAISGVTASA"
     gene            complement(6026..8812)
                     /gene="leuS"
                     /locus_tag="VFV99_30215"
     CDS             complement(6026..8812)
                     /gene="leuS"
                     /locus_tag="VFV99_30215"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5063706.1"
                     /translation="MAGFDHKTIDQKWQQYWDKEQTFLTPTDRTKPKYYVLDMFPYPS
                     GDGLHVGHPKGYTATDVVARAKRMMGFNVLRVMGWDSFGLPAERRAERTGEHPSVITK
                     RNIATFKSQLKKLGLSYDWTRELATSEPDYYKWTQWIFEVLFKRGLAYRAEVPVNFCP
                     ALGTVLANEEVKDGVYIETGDPVEKRRMLQWMLKITEYADRLSEDLNGLDWPNGTHKA
                     QFDWIGKSIGANVVFKLVGSGEPITVFTTRPDTLFGGTWVVLAPEHALVDQITTPEQR
                     DAVAKYREETSKRSERDRTTEAADAPKTGVWTGAYVINPVNGKQIPLWIADYVLASYG
                     TGAVFACPAHDERDHAFAKKFGLPIIEVVKSPVDVQKEPYTGEGAHVNSDFLDGLNVE
                     DAKAKVIAWLASKGLGEASIRYKLRDWLFSRQRYWGEPFPVYIADDGTPKLVPTSDLP
                     VALPKIDEYKPAPDGQPPLARAKDWLRYRNPETGETGTRETNTMPQWAGSCWYYLRFL
                     SPHRDDVAWDPEEEKYWMPVDLYVGGNEHAVLHLLYARFWHKVLFDAGFVSTKEPFQR
                     LFHQGMIHKTSFRDQKGKYYTDQEVEERSQLESRSSLTGSYTDNKSAWLFVQETDGQV
                     IGNGRIGSVELKFAGPATTTIAGTTSTDYPFTLAFADGKPVVTIGKVKTEFFPRPMAM
                     YVKGQDVVLETKLDKMSKSRYNVVNPDDMAAEYGADAMRLYELFMGPLEDGVEWETNG
                     VAGTRRFLDRAWRLLVDPETDTLTTKVSEDAPTTNADLERALHAAIKKVTQSITDLRF
                     NTAISEMMVFVNEATKATAVPKAWFESFVKILSAFAPHVGEELWQRLGHRGSIAYQPW
                     PAFDESKLARDTMVIGVQVSGKLRGQIEVPSDATEQTILAAAKADDKVAAFIAGKPIK
                     REIYVKGRLVNLVV"
     gene            complement(8812..9435)
                     /locus_tag="VFV99_30220"
     CDS             complement(8812..9435)
                     /locus_tag="VFV99_30220"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5063707.1"
                     /translation="MRMILVGPPGAGKGTQAARLVEEFRIPHVSSGDMFRAEVKEGTE
                     LGKKLDHYMKAGQLVPDDVTIQIVLRRITQADCAKGFMLDGFPRTRPQAEALEAAMKK
                     AGVDLDAVVLIEVPDSLIVDRITGRRSDPKTGAIYHTKFNPPPAGLEVVQRKDDTVEA
                     VTTRLAKYHAETAPIIPYYAEMGILKRVDGVGEQDTVTERIKAVLND"
     gene            9501..9875
                     /locus_tag="VFV99_30225"
     CDS             9501..9875
                     /locus_tag="VFV99_30225"
                     /inference="COORDINATES: protein motif:HMM:NF026420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HEX5063708.1"
                     /translation="MKPVPRWFVAVCWVLAVLFAISVGLQVNDPDPVRWMLIYGLAGL
                     AIGLLPARRYLCLACFAIALFAAGWAAYLSQQVFGVIHFSDLWLKMSEKGGAVEVGRE
                     AGGLTIIAIGLVACGIFRSTRA"
     gene            complement(9856..10299)
                     /locus_tag="VFV99_30230"
     CDS             complement(9856..10299)
                     /locus_tag="VFV99_30230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063709.1"
                     /translation="MDLTWHVRTFAELSTVELYAIMALRQRVFVVEQNCPYLDADGYD
                     QVSRHLWAARGDTIAAYLRIVPAGTKFAELSIGRVITAPEARGSGLGRELMKRGIEAA
                     GAVPIRLGAQAHLEKFYGELGFARVGDVYDEDGIPHVDMLKPASI"
     gene            10324..>10679
                     /locus_tag="VFV99_30235"
     CDS             10324..>10679
                     /locus_tag="VFV99_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5063710.1"
                     /translation="MQSVCVFLSSSLGLAAHATAMQELAAEMVRRKLTLVYGGAHRGL
                     MGVLADAVLAHGGRAIGVIPRALVDLEVAHTGLTELHVVETMHQRKAMMFTLADAFLV
                     APGGLGTLEEAFETMTG"
BASE COUNT         1711 a         3568 c         3410 g         1990 t
ORIGIN      
        1 ccgattcggt cggcctcgcg attgcgggca gcttttagcc gtatcgggcc gcggtcgccg
       61 gcgacgctgt cgacagcgac cgcgagcacg aaacgaaatg cgacgccgcc atgagaggga
      121 ggctggcggc gccgcgagaa ctacttccgg cgccggcgcg agtagccgac gacgatcacg
      181 accgcgaaca cgatcggtgc ccacgcgcgg ccgtcgctct tgcccgcgtt gagactgcac
      241 gccggcagat cgccggggcc gctgccgtcg tcgtcgccac caccgccgct gccacccggg
      301 ccaggcacct tcaccatcgc cttgacggtc gtcacgttgc cgtcgccgtc ggtcgcgctc
      361 gcttcgagct cgtgcatacc ggcgcccgcc gcgctcgcgt cccagtcgaa ctgatacggc
      421 gcttccatgt ccatgccgac cgacgtgccg tcgatcttca gctcgacgtc cgtcaccgca
      481 acgtcatcgc ccgccgccac gaccaccacc atcataccgg tcacctgatc gccgtcggcc
      541 ggcgacacga tctcgatcgt cggtgccgcg ccggtgccgc cactgcccga gccactgccg
      601 ctaccgccgc cgccacctgt gagcccgaag aacttcgccg cgcgtagcgt cgcgcagatg
      661 cccttcgctt cgaagtacga gccggccgtt gccgggcaca ccgtcgagcc ttcgttgccg
      721 acggcaaccg cgtggctcat gccgttgacc gtgtacgact cgacgacgat cgtcgcgccc
      781 atcttgtatg cggtgcgcgt cgcggccgag ccgatcatct cggtctcgtc ggcggtcatg
      841 tcggtgccga acacgttcgt ccactgcttc tgcagctcgg tctggttcgc tggcacgacg
      901 gtcgtgtcac tcgagccgtg ccagatctgg atgcgcggcc gcgtgccgct gaacgtgtat
      961 gcaccgcgca cgaggtcgcc ccactcttgc ggcgtgtgcg tgacgcccgg gctctgacag
     1021 ctgtacgcgc cgttgacgct ggtcgcgcac ttgtacgcga tgccttccat gaccgccgcc
     1081 gctgcgaagc gctcgggcca cgtcgcgagc atcaccggta cgaacgcgcc gcccgccgag
     1141 aagcccatga tgaatgtctt cgcgttgtcg gtgccgtgcg tcgtgtgcat gtagtcgatc
     1201 atcgagatga tcgattggtt ctcgcccatg ccacgcatga ggttcgcggt gtcgccgtac
     1261 tcgcccgccc agttgaagca cttcaccggg ttgttcgccg acgtctgctc gggatagagc
     1321 accgcgaact ggtactgatc cgcgagcttg ttccagcccg cgttctccat gttcgcggcg
     1381 gtctgggtac agccgtgcag cacgacgacg agcggtctgc ccgtcggtaa gttgggcggc
     1441 acgtattcgt acatgttcag cgcgccggga ttggaaccga agttgctgac ggcggtaagt
     1501 gcggcgtcgg caccagacgc ggtcacgagc acgaagagcg cggcaaccat gaaacgaaac
     1561 atgcgaatcc tccgcgaccg cgctgtgcac gcgatgtgcc gcatgatcga tgaacgcaag
     1621 acggtgatca gacgcggcga cctgttggct gagtccaaca ggcaagattt ccgtcgtggg
     1681 cgcgagccat cggtgataac gatcgacgcg atggtcgata cgcctcgcac gaagctgcaa
     1741 ccgagacccg tgcgcctcgt gcacaagcta cgcgtcaccg tgaatgcagg cgtcgacgcc
     1801 ggcgcgacgt gcgcacccga tgatggctcg cgcctcgcga tcggtacgag cgaagacaac
     1861 gcgctcgtgc tcaccgatgc agcggtcagc cgctatcacc tcgagctctt gcgcacgccg
     1921 gctggtgtcg aagtgatcga tctcggcagc cgcaacggta cgtgggtcgg tggcgtgcgc
     1981 gtgtcgcgtg cgatcgttcc cgccggaacg cagctccgcc tcggcgacac gacgctcacg
     2041 gtagaagacg cgggctcgac cgtcgcgccg ccgccgccga gcgacgtgcc gcgctcgacc
     2101 gatctggttg gcgagagcga tgcgattcgc gaagtcgcgc gtctcgtgca tcggctcgcg
     2161 cgcgtcgatt catccgtgct catccagggc gagaccggaa ccggcaagga agtcatcgcg
     2221 cgtgcgattc acgaagcgag cccgcgccgc gatcacgagc tcgtggtcgt cgattgcgga
     2281 tcgatgccgg cctcactgat cgcatcgatc ctgttcggtc acgagaaggg cgcgttcacc
     2341 ggcgccgatc agcgccgcgc gggtgcgttc gagcgcgcgg ccggtggcac cgtgctgctc
     2401 gacgagatcg gcgagctgcc gctcgatgtc caaccggcgt tgctcggcgt gctcgagcgc
     2461 cgcgcattca cgcgcgtggg tggagcgcaa ccgatcgcgg tcgatgtccg cgtcctcgcc
     2521 gccacgcacc gcgatctccg cgccgaggtc aacgcgggtc ggttccgcgc ggatctgtat
     2581 taccgcttgg cagtagcgaa ggttctgatc ccgccgctgc gcgagcgtcc ggaagacatc
     2641 gagccgctcg tcgcgcactt cgtgcagcgg cttacaggcg tagccgagct cggtccactc
     2701 gcgagcgcgc tcgaccagct ccgcgcgcac ccgtggagcg gcaatgtccg tgaattgcgc
     2761 aacgtcgtcg aggccgcgct ggtgatgggc gagctcgatc tcggcaccgg cgccgcacac
     2821 accgcgcagc cgatcgtcgc gacgaacggc gctgcgatca cgccataccg cgatgcacgt
     2881 gcaaccgcgc tcgcgcgctt cgaggccgac tatctgcgca cgttgatcga gcgcgccgag
     2941 ggcaacgcgt cggaagctgc acgcatcgca cgcatggatc gtccgtatct gctcacgctg
     3001 ctgcggaagc acggactgcg cagctaacgg acaccgtcac cgactgcccc tgggagtttt
     3061 tactgagggg aacagtcccg tgtaccaagt ctgcgttcgt gtacgacggg ttcacacgag
     3121 ttgtcgctgc aacgtgcttg aaacacttcg acgctgattc gcaaagtgca ggcacgaccg
     3181 ctgcactcgc gctcgtcacc aaaggagccg cccgatgacg aagctaattg gtctcaagcg
     3241 tctggtcctg atcatgatcc ccgcgatgac aatcgcgatg gcccccgcat gcacgacgat
     3301 gctgggcagc gatacgtccg ccgcatcgaa gatgggcgcg gatgacagcg acaccgacag
     3361 cgacagcgac gacggcgata gcgattgctc gtcggtcgac gaggattcgg acaccgacgg
     3421 tgataccgac gagcccggcg ataccgacag cgacaacgac agcgatagcg atagcgactg
     3481 cggcggtggt ggcggaagcg gctcgggcag cggaagcggc tcgggcagcg gaagtggtag
     3541 cggcgggccg gtataatccg gctcgatgct ggcgacgctc gcattgatcg cgctgggtgc
     3601 ctcgctcgtg cccgcgcacc gtgcggcgcg ttcgtcgccg gctctgctca ccgcactgat
     3661 cacgaccgaa gccatcggcc tcgtgctcgt cggcgtcgcg gtcgtaagcg cgcgcacctg
     3721 gtcgtccatc gcgcaggagg acggcgtcat cgaatgggcg acattcctcg cgtatgtatt
     3781 cgccgcgggc tggctgctcc tcgtcgtccg caagcaatcg ccgtcgtggt ggttcaaggg
     3841 cgcgacgttc ttgctcgcgg cgttctgcat cgtcgtcgcc ggcgaagaga tttcgtgggg
     3901 acagcggctg ttcggcttca agccgcctga cgtgttcctc gagcgcaact ttcagcagga
     3961 gatgaacctg cacaacgtgc tgatggacga gcgtggtctc gggttcaagc tcgagtccaa
     4021 ggacctcgtc gtcgtcgtcg tcctcggctt ctgcgcggtg tggccgtggc tcgtgcgccg
     4081 gcgccgcttt accgtgttcg ggccgctcgc accgccgtac gcgctgttgc cacttgcgct
     4141 cggcgtggcg gccgccgagc tcgcgtacaa cgtcgagctg accggcgaag gcgcggagct
     4201 cgtcgctggc ctcatcttcc tcgcgtcggc gctcgtcgtc gcgcagccac caccacgcgt
     4261 cgtgatgacg tggctgctcg cgccgctcgt cgtcggcctc ttgctgtcga cgatcctcgc
     4321 gcgcgtgatg ttcggcagcg acgaagacgg tacgcgcacc gccgccgccg agctcgcgct
     4381 gctctcccaa gacgtcgcaa acggtgcgac cgacaagctg ctcaagaaga acgtgcacaa
     4441 gcgcgcgttc acgtcgatgc acgacggcta cgtgacactc gccggcggca cgttttacga
     4501 gggtcaagcg aagcgtcacg atcgacgcgg ctactgtttg gatccctgga acaacccgta
     4561 ctggatcttc tacgacaaga gctctcgcac cggcgccgtc tattcattcg gcccgaatcg
     4621 ccgccgcgac atcgtggtgc gcgatccgaa aagcgatcca ggcgacgacg tgatcgtgcg
     4681 cttcaggatt ccggagacgg ccgccgcagc accacccgag cagcctgcgc agccggacga
     4741 cccaacgccg ccgcagcctt agctcttctt ttcgttcttg atccgcggca gcagcatcac
     4801 gaagttgcac ggcttcagcc ggtgatcgag ctgcggcacc aagatcttgt cgacgccgag
     4861 cttcacccca ccggtcgagc ccggcagcaa gaacacgtac gtctgtccgc acagcgcggc
     4921 atcggctcga ctctggatcg tcgcggtccc gatctccgcg aatgacagct gacgaaacag
     4981 ctcgccaaag ccggggatat gtttcgtgat gagcggtgcc agcgcctcgg gcgtcacgtc
     5041 gcgtggagtc acgccggttc cgccggtcgc gatgatcacc tcgacctcac cgccctcgat
     5101 ccatgcgcgc atctgctccc gaatcgcgct ctcgacgtct ttgacgatcg ttcgcgcgac
     5161 gatccggtgg ccctcctgag acagccggtc agcaatgtac ttgcccgacg tgtcgttcgc
     5221 ctcggttcgc gtatccgaga cggtcagcac cgcaacgttg accggaatga actcgcgctc
     5281 catgcgtctg gagtgttgca cgatcgtcat tttcctgagt agtgaacatt gtttaacgca
     5341 acgcgttagt tgctgttagg atgctaggac tggcgcgatg acgcgatgcg tcttagtgat
     5401 gggtgcaagg agatacgcac atggcgacga aactcgaagc aaccaccagc ccgaccttca
     5461 cccgcaccga ccgcaccggc tttgtaccgg gcgtcgcgaa cctcgctctc gatgtggccg
     5521 accgcggcca gagcaccgtg atcgcggtcc tgcaggatgc gcgcggtgag ctccgcacgc
     5581 tcgtcgagaa tggcatcgag ctcgccgaga agacggcagc tcaggcgttc cgcttcgcgc
     5641 gcacgctcac caagcgcgtc gatgacgcgg cggcggagac gctgaacaac accgagcgcc
     5701 tgctcggcaa cgcggtccgc tcggcgcgtg agacggcgaa gtcggcgacc gataccgcca
     5761 acacggcgat ctcgggcgtc accgcttcgg cgtaattcgc gaggcgatcg aaacgatcgc
     5821 cgatcgcagc gtctgattga tcgattctgg cgaatccccc tccatcccta cgtctcggga
     5881 ttgatcgatc agacactggg ttcggcgatc aagcgcggcg ccttgtcggc gccctgtgat
     5941 ccaacgaggt gacgacgtcg atccccccgt cgacgaagcg tccctcgacg ttgatctcgc
     6001 gcttgatcgg cctgcccgtt cggcgctaga cgacgaggtt gaccaggcgt cccttgacgt
     6061 agatctcgcg cttgatcggc ttgcccgcga tgaacgcggc caccttgtcg tcggcctttg
     6121 ccgccgcgag gatcgtctgc tccgtcgcat ccgacggcac ttcgatctga ccgcgcagct
     6181 tgccggagac ctgcacgccg atgaccatcg tgtcgcgcgc gagcttgctc tcgtcgaacg
     6241 caggccacgg ctgatacgcg atcgagccgc gatggccgag gcgctgccac aattcctcgc
     6301 cgacgtgcgg cgcgaatgcc gacaggatct tcacgaagct ctcgaaccac gccttcggca
     6361 ccgccgtggc cttcgtcgct tcgttgacga agaccatcat ctcggagatc gccgtgttga
     6421 agcgcagatc ggtgatcgac tgcgtgacct tcttgatcgc cgcgtgcagc gcacgctcga
     6481 ggtcggcgtt cgtcgtcggt gcgtcttcgg agaccttcgt cgtcagcgtg tcggtctcgg
     6541 gatcgacgag cagacgccac gcgcgatcga ggaaccgtcg cgtacccgcg acgccgttcg
     6601 tctcccactc gacgccgtct tcgagcggtc ccatgaacag ctcgtagagg cgcatcgcgt
     6661 cggcgccgta ctcggccgcc atgtcgtcgg gattgacgac gttgtagcgg ctcttcgaca
     6721 tcttatcgag cttggtctcg agcacgacgt cctgcccttt gacgtacatc gccatcgggc
     6781 gagggaagaa ctcggtcttc accttgccga tcgtcacgac gggcttgccg tcggcgaacg
     6841 cgagcgtgaa cggatagtcg gtcgatgtcg tgcccgcgat ggtcgtcgtc gcgggtccgg
     6901 cgaacttgag ctccacgctg ccgatgcgcc cgttgccgat gacctggccg tcggtttcct
     6961 gaacgaacag ccacgcgctc ttgttgtccg tgtagctgcc ggtcagcgag gagcggctct
     7021 cgagttgcga ccgctcttcg acttcctggt ccgtgtagta cttgcccttc tgatcacgga
     7081 atgacgtctt gtgaatcatt ccctgatgaa acaggcgctg gaacggctcc ttcgtcgaga
     7141 cgaagccggc gtcgaagagc accttgtgcc agaaccgcgc gtagagcagg tggagcaccg
     7201 cgtgctcgtt gccgccgacg tagaggtcga ccggcatcca gtacttctct tcttccggat
     7261 cccacgcgac gtcatcgcga tgcggtgaga ggaagcgcag gtagtaccag cacgaacccg
     7321 cccactgcgg catcgtgttt gtctcgcgcg tgccggtctc gccggtctcg ggattcctgt
     7381 agcgcaacca gtccttcgcg cgcgcgagcg gcggctgacc atccggcgcc ggcttgtact
     7441 cgtcgatttt tggcaacgcg acgggcaggt cgctcgtcgg cacgagcttg ggcgtaccgt
     7501 cgtcggcgat gtacaccggg aacggttcac cccagtaccg ctgacgcgag aacagccagt
     7561 cgcgcagctt gtagcgaatg ctcgcctcac cgaggccctt actcgcgagc cacgcaatca
     7621 ctttcgcttt ggcgtcctcg acattcagac cgtcgaggaa atcgctgttc acatgcgcgc
     7681 cttcgccggt gtacggctcc ttctgcacgt cgaccggcga cttcacgacc tcgatgatcg
     7741 gcaggccgaa cttcttcgcg aacgcgtggt cgcgctcgtc gtgcgcgggg cacgcgaaca
     7801 cggcaccggt gccgtagctc gcgagcacgt agtccgcgat ccagagtggg atctgcttgc
     7861 cgttcaccgg attgatcacg tacgcgcccg tccacacgcc ggtcttcggc gcatcggcgg
     7921 cttccgtggt gcgatcgcgc tcgcttcgct tcgacgtctc ttcgcgatac ttcgcaacgg
     7981 cgtcgcgctg ctcgggcgtc gtgatctgat cgacgagcgc atgctcgggc gcgagcacga
     8041 cccacgtgcc accgaacagc gtgtccggac gcgtggtgaa caccgtgatc ggctcgccgc
     8101 tgccgacgag cttgaacacg acgttcgcgc cgatcgactt gccgatccaa tcgaattgcg
     8161 ccttgtgcgt tccgttcggc cagtcgagac cattcagatc ttcgctcagc cgatcggcgt
     8221 actcggtgat cttgagcatc cactgcagca ttcggcgctt ctcgacgggg tcgcccgtct
     8281 cgatgtacac gccgtctttg acttcctcgt tcgccagcac ggtgccaagc gccgggcaga
     8341 agttgaccgg cacctcggcg cgatacgcga ggccgcgctt gaagaggacc tcgaagatcc
     8401 actgcgtcca cttgtagtag tcgggctcgc tcgtcgcgag ctcgcgcgtc cagtcgtacg
     8461 agagaccgag cttcttgagc tggctcttga acgtggcgat gttgcgcttc gtgatcacgc
     8521 tcgggtgttc acccgtgcgc tcggcgcgtc gctcggcggg aaggccgaag ctgtcccagc
     8581 ccatcacgcg cagcacgttg aagcccatca tgcgcttcgc gcgcgcgacg acgtccgtcg
     8641 ccgtgtagcc cttcgggtgc ccgacgtgga ggccgtcacc cgacggatac gggaacatgt
     8701 cgagcacgta gtacttcggc ttggtgcgat ccgtgggcgt caggaacgtc tgttccttgt
     8761 cccagtactg ctgccacttc tgatcgatcg tcttgtggtc gaagcccgcc attagtcgtt
     8821 caacaccgct ttgatgcgtt ctgtaaccgt gtcctgttcg ccgacgccgt caacccgctt
     8881 caggatgccc atctcggcgt agtacggaat gatcggtgcg gtttcggcgt gatacttcgc
     8941 cagacgcgtg gtcaccgcct cgacggtatc gtccttgcgc tggacgactt cgaggccggc
     9001 cggcggggga ttgaacttcg tgtgatagat cgcgccggtc ttcggatcgc tgcgccgacc
     9061 cgtgatgcga tcgacgatca agctgtcggg gacttcgata agcacgaccg cgtcgagatc
     9121 gacgccggcc ttcttcatcg ccgcttccag cgcctcggct tgcggccggg tgcgaggaaa
     9181 accatcgagc atgaaacctt tggcgcaatc ggcctgcgtg attctacgta gaacgatctg
     9241 aatcgtgacg tcgtcgggca cgagctggcc ggccttcatg tagtggtcga gcttcttgcc
     9301 gagctcggtg ccttccttga cctcggcgcg aaacatgtca ccgctggaga cgtgagggat
     9361 ccgaaactct tcgaccagac gagccgcctg cgttccctta ccagctcccg gtggaccgac
     9421 gagaatcatg cgcatggggc gcatccaagc atgtgtaccg cacttctgca atgtggcgtc
     9481 cgctgcgtta tggtcagcgc atgaagccgg tcccgcggtg gtttgtcgcc gtctgttggg
     9541 tgctggcagt gctgttcgcg atctccgtcg gcttgcaggt aaacgaccct gatccggtcc
     9601 gctggatgct catttacggg ctcgccggtc ttgcgattgg cttgctcccg gcgcggcgct
     9661 acttgtgcct cgcgtgcttc gcgatcgcgc tgttcgccgc gggctgggcc gcgtacctga
     9721 gccagcaggt attcggcgtg attcacttca gcgatctgtg gctcaagatg agcgagaaag
     9781 gcggcgcggt cgaagtcggc cgcgaagccg gcggcctcac gatcatcgcg atcggcctcg
     9841 tcgcgtgcgg gatcttcaga tcgacgcggg cttgagcatg tcgacgtgcg ggatgccgtc
     9901 ttcgtcgtag acgtcgccca cccgcgcaaa gccgagctcg ccgtagaact tctcgaggtg
     9961 cgcctgcgcg ccgaggcgga tcggcaccgc gcccgcggct tcgatgccgc gcttcatcag
    10021 ctcgcgacca agcccgctac cacgcgcttc cggcgccgtg atcacgcggc cgatgctgag
    10081 ctcggcgaac ttcgtccccg ccggcacgat gcgcaggtac gccgcgatcg tgtcaccgcg
    10141 cgcagcccac agatgacgcg agacctggtc gtagccgtcg gcgtcgaggt acgggcagtt
    10201 ctgctcgacg acgaacaccc gttggcgcag cgccatgatc gcatagagct cgacggtgct
    10261 cagctccgcg aaagttcgca cgtgccaggt gaggtccacg ccgcatcata gtacggtcgc
    10321 atcgtgcagt cggtgtgcgt gttcctgtcg tcatcgctcg ggttggccgc gcatgccacc
    10381 gcgatgcagg agctcgccgc ggagatggtc cgacgcaagc tcacgctcgt gtatggcggc
    10441 gcgcatcgcg gcctgatggg cgtgctcgca gacgctgtgc tcgcgcacgg cgggcgcgcg
    10501 atcggcgtga ttccgcgcgc gctggtcgac ctcgaggtcg cgcacacggg cctgaccgag
    10561 ctgcacgtcg tggagaccat gcaccagcgc aaggcgatga tgttcacgct cgccgacgcg
    10621 tttctcgtgg cgcccggagg cctcggcacg ctcgaagagg cgttcgagac gatgaccgg
//
LOCUS       DASUFX010000748         9033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1370488, whole genome shotgun sequence.
ACCESSION   DASUFX010000748 DASUFX010000000
VERSION     DASUFX010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370488"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1139
                     /locus_tag="VFV99_30240"
     CDS             165..1139
                     /locus_tag="VFV99_30240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5063711.1"
                     /translation="MRALVVAALVLACGVARADTRFQLTAWNMTIKTADGSTQMTVFG
                     DDPNKLLPSCRCVAGVWQPAKSGESATHFKGTLKALSINYPRGKSKTLTVKVRKTRYL
                     MGANRDYRPALLAILRGSGQVIVREVTGGEWEVVGFAPQPLGDLFAAHPRWRVDAKKP
                     PKPTPANAIVNPDADATELAKLINDYRETLKLPRVPVSKALTKVARVHVHDLTDNKPT
                     SDKCNMHSWSSKGSWSACCYDKSKEAAKCMWAKPKEIAGFAANGYEIAASASGIAPQQ
                     ALEQWQHSPAHHEVMINKGIWTKPWGAIGVAIEGDYAVAWFSEEADKK"
     gene            1215..2075
                     /locus_tag="VFV99_30245"
     CDS             1215..2075
                     /locus_tag="VFV99_30245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063712.1"
                     /translation="MRLAIPTTEPFSFAQTLAFIERFPPCRDEYVMTENSLTAAISIG
                     GKPYAFTLHDGRAIEVDDATPPAVANELVTRAAQFIGATDDLEAFYAATRGDEPMQRV
                     VKLLHGLHHVRFLTLGEVAVYSVMMQRSPMTIAGTYKRRFTEAFGVPVTVDGHTLRAW
                     PSLDELVTLPLADVARALRHRPKAERIVEVVRGVHALGEKFLREAPYAKARDALIEIP
                     GVGPFAAGAILLRGLGRMDELPWSDRFVNEAKPLYGRRVSQAEIENRYGPSIGYWSFY
                     MMTGTPRLTA"
     gene            complement(2065..2721)
                     /locus_tag="VFV99_30250"
     CDS             complement(2065..2721)
                     /locus_tag="VFV99_30250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063713.1"
                     /translation="MFRRLPHHLLALVVVLPACVNHLPPAPTPAAVAPPMQPAPPSAP
                     GMGRLLVDVTDGPVRVQRVNMTSRPIDAGQGRTVYRFTEEPVTLCAVSPCATDVSTGN
                     LLLGFPVIGKPNSLEVELVHVGPEPSVYRRTLSVYTDHGSNVFGVLMTTFGGVGLATG
                     ASLLPIGLATETGGMTFAGGISLGVGTALLVIGIVSLRNHADTFRPGSSNHFPLAATP
                     "
     gene            2739..3668
                     /locus_tag="VFV99_30255"
     CDS             2739..3668
                     /locus_tag="VFV99_30255"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063714.1"
                     /translation="MRAWSLLLVGCVACGGRLPAPGQIGIPVKLTTRIDAHIDLRVRS
                     EAQVSVQGQGQAGQAEPPPPPPPPPPVQIVAVENAPVPEFFGISLEGAQDVVFVLDVS
                     GSMDEKAFGRLAQIHVTAAAANTPPAEPPPPPPDPNAPPPPPPDPNAPPPDPNAPAAA
                     APPAPEPPAPRKIDVAQSELIAALRQLPAGTRMNVLFFNDALDAYAPSFVVLDEATRT
                     TLITFVDDSEPMGSTALAPAMRTAFLMNAHRIVLLSDGLGNVGGNADAVMRDAREAMR
                     GGVRIDTIGLGAGQDARLLGALAKESGGLYQPM"
     gene            3681..4406
                     /locus_tag="VFV99_30260"
     CDS             3681..4406
                     /locus_tag="VFV99_30260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5063715.1"
                     /translation="MKQADHVTATIVRPSRESGAFAVRDLSPGGARLVGPLDLFEGEL
                     VQLTLELDEPLTLGAEVSHVDRQRKVVEVAFRGVTEAALARIEHSIAEMLERVQASAP
                     TVLIVHPAVDVSSALERDLARVRVAARVCVKLDELFAVLGDKTVRFIGVIVAGSFGNA
                     LGPVLQELEEKRGDLRRVILFGDQIEKIEHPAANRVDAVLRTPWRFKGLARGLGVPAE
                     DVVTTYDQLVALNMEIGVKKSDS"
     gene            complement(4408..4623)
                     /locus_tag="VFV99_30265"
     CDS             complement(4408..4623)
                     /locus_tag="VFV99_30265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063716.1"
                     /translation="MYPVRRSRKLLWFFVGLFVTTTLAAGAVVAYPALLDPLCDDYEW
                     FGNDAALVVREQAREAHTAIVDFVSNL"
     gene            5429..5992
                     /locus_tag="VFV99_30270"
     CDS             5429..5992
                     /locus_tag="VFV99_30270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5063717.1"
                     /translation="MKKGSTRERRRVRKVLLVDDMEMVLKVWTRQTRRQGKVPLSATN
                     RAEALAIARREKPDLAIVDLIIPNDSGIEIVKELKKLRPKPFVILVSGAMSAEAAMQG
                     VEAGADDCYDKDVTVTDLIERVESGVRPREWQQPTLKEMQWQYIRRVLTDHGGNITHA
                     AKVLGVVRQSLQRTISRLSPTGRPPVP"
     gene            6050..6796
                     /locus_tag="VFV99_30275"
     CDS             6050..6796
                     /locus_tag="VFV99_30275"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5063718.1"
                     /translation="MGVVDGAIVALFAMLAVLIAIEHVRPVRAFEEVRGWKLKCVGFM
                     PIVIGISASIPYLMASVIADVKLLPGDQLGIVGGTVIGIVFSELLVYWAHRLHHRVRF
                     LWRWIHQLHHSAERVDVFGAAYFHPFEIIEGTVIGVLVFNVVLGLTPEAAFLSGVWQA
                     FNGVFQHGNIRTPTWLGYFVQRPEAHAIHHERGIHDFNYANLPLWDIVFGTFRNPAAW
                     QGTAGFYSGASKETLRMLIGRDISTGATRT"
     gene            6793..7401
                     /locus_tag="VFV99_30280"
     CDS             6793..7401
                     /locus_tag="VFV99_30280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063719.1"
                     /translation="MSRPLRVMLYDRSCRGRGLALGGLSHAWSFGGLLYRALGRIDAW
                     HGAWSWSDGLDWFVEISRTRPIAEIQFWGHGEWGGLWIDEELLTAAALEPDHYLHARL
                     AAVKARLANDALWWFRSCDVFGTQIGHDFARAWTRYFNCRAAGHTHTIMFVQSGLHML
                     APNEEPTWSLDEGVVDGLAHASESSLRAPRTITALHNAIPPR"
     gene            7403..8071
                     /locus_tag="VFV99_30285"
     CDS             7403..8071
                     /locus_tag="VFV99_30285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5063720.1"
                     /translation="MEQPNPPTQLRKAYVPALGFHWLTALYDPLIRSWSAAARMRRAV
                     IDALDLRAGLRILELGSGPGRLAIQIKRGHGEVTIDAVDIDERMVASAKHNAAGAGVD
                     IEFREADMTCLAEHGTYDRVYSTMTFHHLMPDAKYDALAAARRALAPGGRFVIADFGR
                     PRDPLQWALFAWIQQPLDGFQNTRPHRDGRFERALRDTFSHVHSAEAWRTVAGTVELF
                     VCSA"
     gene            complement(8073..>9033)
                     /locus_tag="VFV99_30290"
     CDS             complement(8073..>9033)
                     /locus_tag="VFV99_30290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5063721.1"
                     /translation="IARWIGQHVHDCELAPATLALQADSRASDAALREFARAATIAGL
                     EHRLLDSRVRPGHVVLEMDAYTLHPYKLVRGLAEHATANDVMIRERARARKVEGLRAG
                     GARVHLDDGTTIEARSKVVVCTNAYTSSIDLEERVRALAVHSFMTATAPVAAELVRDG
                     DFTVEINTTQAYHRMHHGRIIFGGIDKLRAPEGDDFAVPAREQARLAQQMAASFPGVD
                     LAIEHRWSGRFHATATGLPIIRTAERPDNAIILNVGYGGTGVALSLVCARLAAAVASR
                     GQFMNADDPRLLALMHATRISVRDSVRALARIARGVAMPWLRA"
BASE COUNT         1580 a         2857 c         3091 g         1505 t
ORIGIN      
        1 ggcgccgcac acgcgcggcg cgatcgcgca gaacgatcga gcgacccgct acttattcct
       61 cacaccacgt cttgaacacg cacgcatcct acgacgcgtc ctgagcccgc agccggtttt
      121 tgccacttcg ggcattccga gatccgctcg ctatactcga tggcatgcgc gcgctggtgg
      181 tcgctgctct cgtgctcgcg tgtggtgtcg cgcgcgccga cacgaggttc cagctcaccg
      241 cgtggaacat gacgatcaag accgcggacg gctccacgca gatgaccgtg ttcggcgacg
      301 atccgaacaa gctgttgcca tcgtgtcgat gcgtcgctgg cgtctggcag cccgcgaaga
      361 gcggcgagag cgcgacgcac ttcaaaggca cgctcaaggc gctgtcgatc aattacccgc
      421 gcggcaagtc gaagacgctc acggtcaagg ttcgcaagac gcggtacttg atgggcgcga
      481 atcgcgacta ccgtcccgcg ctgctcgcga tcctgcgggg ctcggggcag gtgatcgtgc
      541 gcgaggtgac gggcggcgaa tgggaggtcg ttggcttcgc gccgcagccg ctcggcgatc
      601 tgttcgccgc gcatccgcgc tggcgcgtcg acgcgaagaa gccgccgaag ccgacgcccg
      661 cgaacgcgat cgtcaatccc gacgcggacg cgaccgagct cgcgaagctg atcaacgact
      721 atcgcgagac gttgaagctg ccgcgtgtcc cggtatcgaa ggcgctgacg aaggtcgcgc
      781 gcgtgcacgt gcatgacctg accgacaaca agccgacgag cgacaagtgc aacatgcaca
      841 gctggtcgtc gaagggctcg tggagcgcgt gctgctacga caagagcaag gaagcagcaa
      901 aatgcatgtg ggccaagccg aaggagatcg ccggcttcgc agcgaacggc tacgagatcg
      961 cggcgtcggc gagcggcatc gcgccccagc aggcgctcga gcaatggcag cacagcccgg
     1021 cgcaccacga agtcatgatc aacaaaggca tctggacgaa gccgtggggc gcgatcggcg
     1081 tcgcgatcga aggcgactac gcggtcgcct ggttcagcga agaagccgac aagaagtaag
     1141 ctcgaaccga gcgattgtcg ggtggattgt caaacctgac aataggctga caagcacgcc
     1201 tcgtatctag aagcatgcgg ctcgctattc cgacgacaga accgttctcg ttcgcgcaga
     1261 cgctcgcatt catcgagcgg tttccgccat gccgcgacga gtacgtgatg accgagaaca
     1321 gcctgacggc ggcgatctcg atcggtggca agccatacgc gttcacgctg cacgacggtc
     1381 gcgcgatcga ggtcgatgac gcgacgccgc ccgcggtggc gaacgagctc gtgacgcgcg
     1441 cggcccagtt catcggcgcg accgatgacc tcgaggcgtt ctacgcggcg acacgcggtg
     1501 acgaaccgat gcagcgagtc gtcaagctgt tgcacggcct ccatcacgtg cggtttctga
     1561 cgctcggcga ggtcgcggtt tattcggtga tgatgcagcg ctcgccaatg acgatcgcgg
     1621 ggacgtacaa gcggcgcttc accgaggcgt tcggcgtgcc ggtcaccgtg gatggccaca
     1681 cgttgcgtgc gtggccatcg ctcgacgagc tcgtcacgct gcctttggcc gacgtcgcgc
     1741 gcgcgttacg tcatcgcccg aaggcggagc gcatcgtcga ggtcgtccgc ggcgttcacg
     1801 cactcggcga gaagttcctg cgcgaggcac cgtacgcgaa ggcgcgcgac gcgctgatcg
     1861 agattcccgg cgtgggtccg ttcgcggccg gcgcgatctt gttgcgcggg ctcggccgga
     1921 tggacgagct gccgtggagt gatcggttcg tgaacgaggc gaagccattg tacgggcggc
     1981 gggtttcgca ggctgaaatc gagaaccgct acggcccgtc gatcggctac tggtcgttct
     2041 acatgatgac cggcacgccg cggctcacgg cgtagcagcg agcgggaagt gattggaaga
     2101 ccccgggcga aacgtgtcgg cgtgattgcg gagcgacacg atgccgatga cgagtagcgc
     2161 ggtaccgacg ccgagcgaga tgccgcccgc gaacgtcatg ccgccggtct cggtcgcgag
     2221 tccgatcggt agcagcgacg cgccggtcgc cagcccgacg ccgccaaacg tggtcatgag
     2281 gacgccgaac acgttcgagc cgtggtcggt gtacacgctc agcgtgcggc gatagacgga
     2341 aggctcgggc ccgacgtgca cgagctcgac ctcgagcgag ttcggttttc cgatcaccgg
     2401 gaagccgagc aggagattcc cggtcgatac gtcggtcgcg cacggcgata ccgcgcacaa
     2461 cgtcactggc tcttcggtga agcgatagac ggtgcggccc tggccggcat cgatcggacg
     2521 cgacgtcatg ttgacgcgct gaacgcgcac cggtccgtcg gtgacgtcga cgagcagtcg
     2581 ccccatcccc ggcgcagacg gtggcgccgg ttgcatcggc ggagcgacgg cggcgggcgt
     2641 cggtgcaggc ggcaggtggt tgacgcatgc gggtaggaca acgacgagcg cgagaagatg
     2701 atgcggcaag cgtcgaaaca caggtaccct cattgtacat gcgagcctgg tcgttgcttc
     2761 tggtcggttg cgttgcttgt ggcgggcggc tgcctgctcc aggtcagatc gggattccag
     2821 tcaagctgac cacgcgcatc gatgcacaca tcgatcttcg cgtgcgaagc gaggcacagg
     2881 tcagcgtcca aggacaaggt caagcgggtc aagcggagcc gccgccaccg ccaccaccgc
     2941 caccacccgt gcagatcgtg gcggtcgaga atgcgccggt ccccgagttc ttcggcatct
     3001 cgctcgaggg tgcgcaagat gtcgtgttcg tgctcgacgt ctcgggctcg atggacgaga
     3061 aagcgttcgg tcgtctcgcg cagattcacg tgaccgcggc ggctgcgaat acaccgcccg
     3121 ccgagccgcc tccgccaccg cccgatccga acgcgccgcc gccaccaccc ccggatccga
     3181 acgcaccgcc gcccgatccg aatgcgcccg cggccgctgc accgcccgct cctgaaccac
     3241 cagcgccgcg caagatcgat gtcgcgcagt cggagctcat cgccgcgctc cggcaattgc
     3301 ccgccggcac gcgcatgaac gtgctgttct tcaacgatgc gctcgatgca tacgcgccga
     3361 gcttcgtcgt gctcgacgaa gcgacccgca cgacgctgat cacgtttgtc gacgacagcg
     3421 aaccaatggg cagcaccgcg ctcgcaccgg ctatgcgcac tgcgttcttg atgaacgcac
     3481 accgcatcgt gctgctgtcg gatgggctcg gcaacgtcgg tggtaacgcc gacgcggtga
     3541 tgcgcgacgc gcgtgaagcg atgcgcggcg gcgtgcggat cgatacgatc gggctcgggg
     3601 caggccaaga tgcgcgattg ctgggagcgc tcgcgaagga gagcggcggc ctttatcagc
     3661 cgatgtagat gcgctcggat atgaagcaag ctgaccacgt caccgccacg atcgttcgcc
     3721 cttcgcgcga gagcggcgca tttgccgtcc gcgatttgtc gcccggtggt gcacgactcg
     3781 tcggtccgct cgacctgttc gagggcgagc tcgtccagct gacgctcgag ctcgacgagc
     3841 cgctcacgct cggggcagag gtgtcgcacg tcgatcgtca gcgcaaagtc gtcgaggtcg
     3901 cgtttcgtgg tgtcaccgaa gcagcgcttg cacggatcga gcactcgatc gcggagatgc
     3961 tcgaacgagt ccaggcatcc gcaccgacgg tgttgatcgt tcatcccgcg gtcgacgtat
     4021 cgagcgcact cgaacgcgat ctcgcgcgcg tacgggtggc ggcgcgcgta tgcgtcaagc
     4081 tcgacgagct atttgccgtg ctcggcgaca agacagttcg gttcatcggc gtgatcgttg
     4141 cgggctcgtt cggcaacgcg ctcggccccg tgctgcagga gctcgaggag aagcgtgggg
     4201 atctgcgacg cgtgatcctg tttggcgatc agatcgagaa gatcgagcat ccggctgcga
     4261 accgcgtcga tgccgtgttg cgcacgccgt ggcggttcaa gggcctcgca cgcgggctcg
     4321 gagttcccgc cgaggatgtc gtcacgacgt acgatcagct ggtcgcgctg aacatggaaa
     4381 tcggcgtgaa gaaatccgac tcgtgagcta caggttgctg acgaagtcga cgatggccgt
     4441 gtgcgcttcg cgcgcttgct cgcgaacgac gagcgcggcg tcgttaccga accactcgta
     4501 gtcgtcgcac agcggatcga gcagtgcggg atacgcgacg actgcacccg cggcgagcgt
     4561 ggtcgtcacg aacagaccga cgaagaacca cagcaacttt cgcgagcgac gtaccgggta
     4621 catcggacgg accgcacgac tcggattggc ctttcgcttg gcgatcgctg cgccgatcgg
     4681 cggctgcgac cacacaaacg gagatgctgt gggcgtgggc ggcggtgggg cgaccgattg
     4741 aggagggggt cgcggcggaa gcgttcgcgg tggcgcaggc gcaacagtgc gttgctgggt
     4801 cggcgacgca acggatcgtg gcgcaacgat gggcggcgca acgacgggag gcatcggcgc
     4861 aacgacggga ggcgccggcg tggccgaccg catcatgatg gtcggtgcag ccgagaccgc
     4921 cggcatctgg atggcgtgac gaggtgcggg cgggggctgc agcgtttgcg caggcccagc
     4981 gggcaacggc gtaactcggt gacgagtcgg agcttcgtcg aatggaagac ccgctaccgc
     5041 tgccggcgcc aatggcagcg gcgtaagcgc gcgtatcatt cgctgaaggg gtggtgcgtc
     5101 acgcggtagc tcgggtagct gcggtggccg gatcaccgac agcacgatca tcacgacgaa
     5161 aagggccgca gcggctgcga cgcagccagc aaggatgtag agggtgatca ggggcgccat
     5221 cgccggagca gccctagcgg ctggaccatt gccccatggt gcaaaaacgc cgccacggcg
     5281 aattgcagcg gcgttctcgg ccggcctacg atctacgcaa ggttggtggt atgcgcacaa
     5341 ttcggacctg gggaatgaac accactcgcg gcaaacaaac cgaccactac cctgcacggt
     5401 cgaagaatcg cgatacgatg gcgatctcgt gaagaaggga agtacacgtg aacgtcggcg
     5461 cgtccggaag gtcctccttg tcgacgacat ggagatggtc ctcaaggtct ggacgcgtca
     5521 aacccgtcgg caaggcaagg ttccgctgag cgcgaccaat cgcgccgagg cgctcgcaat
     5581 agcgcgtcga gaaaagccgg acctggcgat cgtcgatctg atcattccga acgattcggg
     5641 gatcgagatc gtcaaggagc tgaagaagct caggccgaag ccgttcgtca ttctcgtcag
     5701 tggcgcgatg tccgcggagg ctgcgatgca aggcgtcgag gccggtgcgg atgactgcta
     5761 cgacaaagac gtgacggtca ctgatctcat cgagcgcgtc gagagcggag tacgtccgcg
     5821 cgagtggcag cagccgacgc tcaaagagat gcagtggcag tacatccgtc gcgtgttgac
     5881 cgaccatggc ggcaacatca cgcatgcggc gaaggtgctc ggcgtggttc gtcagagtct
     5941 gcagcgcacg atttcgcggc ttagcccgac gggccgaccg ccagtgccgt agccgaccgg
     6001 cttgtgccgg tccgtttctg catcctggcg atatactggt cgtgccgtca tgggggtggt
     6061 cgacggagcc atcgtcgcgc tgttcgcgat gctcgcggtg ctgatcgcga tcgagcacgt
     6121 gcgtcccgtg cgcgcgttcg aagaggtgcg tggctggaag ctcaagtgcg tcgggttcat
     6181 gccgatcgtg atcggcatct cggcatcgat tccgtacctg atggccagcg tgatcgcgga
     6241 cgtgaagttg ttgcccggcg atcagctcgg catcgtcggc ggcacggtca tcgggatcgt
     6301 gttctcggag ctgctcgtct actgggcgca tcgcctccac caccgcgttc gctttctgtg
     6361 gcgctggatc catcagctgc accacagcgc cgagcgcgtc gacgtttttg gcgcggcgta
     6421 ctttcatccg ttcgagatca tcgagggcac cgtcatcggc gtgctcgtgt tcaacgtcgt
     6481 gctcgggctg acgccagagg ccgcgtttct ctcgggcgtg tggcaggcgt tcaacggcgt
     6541 cttccagcac ggcaacatca ggacgccgac gtggctcggc tacttcgtcc agcggcccga
     6601 agcgcacgcg atccatcacg agcgcggcat tcacgatttc aactacgcga acctgccgct
     6661 ctgggacatc gtgttcggta cgtttcgcaa tcccgccgcg tggcagggga cggccggttt
     6721 ctactcgggc gcgtcgaagg agacgctgcg catgttgatc ggtcgcgaca tctcgaccgg
     6781 agcgacccgc acgtgagccg cccgctgcgc gtcatgctct acgatcgctc gtgtcgcggg
     6841 cgcggcctcg cgctcggcgg tctcagtcat gcgtggtcgt tcggtgggct cttgtatcgc
     6901 gcgctcggtc gcatcgacgc gtggcatggc gcgtggtcgt ggagcgacgg gctcgattgg
     6961 ttcgtcgaga tatcgcggac gcggccgatc gcggagatcc agttctgggg ccacggcgag
     7021 tggggcgggc tgtggatcga cgaggaattg ctaaccgcgg cggcgctcga gcccgatcac
     7081 tatctgcacg cacggctcgc cgcggtgaag gcgcggctgg ccaacgacgc gctgtggtgg
     7141 tttcgcagct gcgacgtgtt cgggacgcag atcggccacg acttcgcgcg tgcgtggacg
     7201 cggtacttca actgccgcgc ggccggtcac acgcacacga tcatgttcgt gcagagcggc
     7261 ctccacatgc tcgcgccgaa cgaagagccg acgtggtcgc tcgacgaagg tgttgtcgat
     7321 ggactcgcgc acgcaagcga atcgagcctg cgcgcgccgc gcacgatcac agccctccat
     7381 aacgcgatcc ccccgaggtg atgtggagca accaaaccca cccacgcagc tccgcaaagc
     7441 ctacgttccg gcgctcggct ttcactggct cacggcgctc tacgatccac tgatccgcag
     7501 ctggagcgca gcggcgcgca tgcgtcgcgc ggtgatcgac gctctcgact tgcgtgccgg
     7561 tctgcgcatt ctagagctcg gctccggtcc gggcaggctc gcgatccaga tcaagcgtgg
     7621 acatggcgaa gtcacgatcg atgcagtcga catcgacgag cgcatggtcg cgagcgcaaa
     7681 gcacaacgca gcgggcgccg gcgtcgacat cgagtttcgc gaggcggaca tgacgtgtct
     7741 cgccgagcac ggcacgtacg atcgcgtgta ctcgacgatg acgtttcatc atctgatgcc
     7801 tgacgcgaaa tacgacgcac tcgctgctgc gcgtcgcgcg ctcgcgccgg gtggcaggtt
     7861 cgtgatcgca gattttggtc gcccgcgcga tccgctgcag tgggcattgt tcgcctggat
     7921 ccagcagccg ctcgacgggt tccagaacac ccggccgcac cgcgatggcc gcttcgaacg
     7981 cgcactgcgc gacacgttca gccacgttca ctcggcagaa gcatggcgca cggtcgccgg
     8041 cacggtcgag ctgttcgtgt gctcggcgtg agctaggcgc gcagccacgg catcgcgacg
     8101 ccgcgtgcaa ttcgcgcgag tgcgcgcacc gagtcgcgca ccgagatccg ggttgcgtgc
     8161 atcagcgcca agagccgcgg atcgtcggcg ttcatgaact ggccgcgcga tgccaccgcc
     8221 gctgcgagcc gcgcacacac gagcgagagc gcgacgccgg tcccgccgta accgacgttc
     8281 aagatgatcg cgttgtcggg ccgctccgca gtgcggatga ttggcaaacc ggtcgcagtt
     8341 gcatgaaaac gccccgacca cctgtgctcg atcgcgaggt cgacgcccgg gaagctcgcc
     8401 gccatctgct gcgccagtcg cgcctgctcg cgcgccggca cggcaaagtc gtcgccctcg
     8461 ggcgcgcgca gcttgtcgat gccgccgaag atgatccggc cgtggtgcat gcggtgatac
     8521 gcctgcgtcg tgttgatctc gacggtgaag tcgccatcgc gcacgagctc cgccgcgacg
     8581 ggcgccgttg ccgtcatgaa gctgtgcacg gcgagcgcgc gcacgcgttc ctcgagatcg
     8641 atcgacgacg tgtatgcgtt cgtgcagacc acgaccttgg acctcgcttc gatcgtggtc
     8701 ccgtcatcga gatgcacgcg tgcaccgcca gcgcgcagac cttcgacctt gcgtgcgcgg
     8761 gcgcgttcgc gaatcatgac gtcgttggct gtcgcgtgct cggccagccc acgcacgagc
     8821 ttgtagggat gcagcgtgta cgcatccatc tcgagaacca cgtggcccgg ccgcacgcgg
     8881 ctgtcgagca gccggtgttc gaggccggcg atcgtggcag cgcgcgcgaa ttcgcggagc
     8941 gcagcgtccg atgcgcgact gtcggcctgt agcgcgagcg tcgctggtgc gagctcgcaa
     9001 tcgtgaacgt gctgaccgat ccaccgcgcg atc
//
LOCUS       DASUFX010000749         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_538796,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000749 DASUFX010000000
VERSION     DASUFX010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538796"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFV99_30295"
     CDS             <1..1219
                     /locus_tag="VFV99_30295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5063722.1"
                     /translation="RPSCTRSTDMDFDPPEAVRPLLAKIERFIADVVMPAEAEVMEKG
                     FIAAAPLLAELRAKCKADGMWGPQLPKDIGGLGLDLVEHGLVSERLGRSPLGHYVFGA
                     QAPDAGNMEILHKYGSPMQKERWLEPLSRGDIRSCFSMTEPENPGSNPTIMSCHARKD
                     GDHYVIDGHKWFTTAADGAAFAIVMAVTNPEAPPHARASMIIVPTDSPGFERVRNIKI
                     MGDPGEGWGSHAEIWYRNVRVPVENRLGPEGAGFMIAQERLGPGRIHHCMRWIGICER
                     VFDTMCKHITRRKIDDEKTLATRQIAQAWVAEARAEIDASRLMVLNAAWTIERKGFQA
                     ARDQVSLIKFYVADVMLRLVDRAIQMHGALGITSDTVLAFYYVHERGARIYDGPDEVH
                     KMVVAKRILARYS"
     gene            1216..2268
                     /locus_tag="VFV99_30300"
     CDS             1216..2268
                     /locus_tag="VFV99_30300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5063723.1"
                     /translation="MIGEDQAKPVREGEELPLPALKAFLESEVGLLGPVRVEQFPGGH
                     SNLTYLVHHGDREYVLRRPPFGSKVKSAHDMGREVAVLSKLAPVYTRAPHVIAYCDSP
                     DVMGAPFYLMERRRGIILRKDLPPNLDHARIAHLCDALVDALVDLHAVDYRAAGLGDF
                     GKPAGYVERQVKGWSERYIGSQTDDIPAMTEVAAWLATHMPADGPPSLIHNDFKFDNV
                     IFDPSLDQIVGVLDWEMATIGDPLMDVGTSLSYWMQADDAAMFQSPIFGITMKPGMLR
                     RADIAARYFDRSGRQTDHLVFYFAFGLFKTAVILQQIYYRYAKGLTQDARFAPFIMVV
                     RGLADHARASIERMSL"
     gene            2334..>4225
                     /locus_tag="VFV99_30305"
     CDS             2334..>4225
                     /locus_tag="VFV99_30305"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5063724.1"
                     /translation="MRCRGALTCIFVAGCALAKPPPPAPPRHTELGLSVTSVTLANGL
                     RVVLVKDPHASEVQVTMRYRVGSADDLEHPGIAHLVEHLMFQQTLGSQTLFAHLEDNA
                     TFFNAVTTFDGTTYISRARPDFLDKLLSIEAVRLGFRCTTINDSAFEREREVVMQELT
                     LKSEAFDLWDSLLRAVYPANHPYAGARDTVKTVSEITRAEACAFADAYYAPSNAALVI
                     SGNLTNEQVETSLGKFLARIAKRVASSPAPVAAVTRLLPHVASAPVDSRVLLVTWPLP
                     LDPGDQIAVRALFSQAAIGVVNNAVKGHVRAIELGDVRAPVIGVAIEVMETESVQDVL
                     DRVQDVFVQLPNELESYMPNLAFDAIRQRTIYEHFAALEDGSERDSRLAVHALAGQNP
                     ADAVAAEQRSLRNLGRHEVGALTRRYLAFDDATLVTLEPTASKKTGHALDVRPAIHDM
                     GLRRSTPEVARAMTPDESIAVPSITATTRVLPNGLKVVLLPLTSVPTVDMRLVFGAGA
                     ADEPVDKRGAALVAGYGLSSDLRNLNDTINFYVAGGSIDADVTAERTSFEVKGVDMHL
                     DYLLAGLRRWVVDGRYSSSADLMVELVERARKRKNENGALIDAWQTAVYGENNPYARA
                     GIASYGT"
BASE COUNT          752 a         1426 c         1344 g          703 t
ORIGIN      
        1 ccgcccgagc tgcacacgga gtactgacat ggacttcgat ccacctgagg ccgttcgccc
       61 cttgctcgcg aagatcgagc gtttcatcgc cgatgtcgtg atgcccgccg aagccgaggt
      121 catggagaaa ggcttcatcg ccgcggcacc gctgctcgcc gagctgcgtg cgaagtgcaa
      181 agccgacggc atgtggggac cgcagctgcc gaaggacatc ggcggtctcg gcctcgatct
      241 cgtcgagcac ggcctcgtct ccgagcgcct cgggcgttcg ccgctcggcc actacgtgtt
      301 cggcgcgcaa gcaccggatg ccggcaacat ggagatcctg cacaagtacg gctcgccgat
      361 gcagaaggag cgctggctcg agccgctctc gcgtggcgac attcgctcgt gcttctcgat
      421 gaccgagccg gagaatccgg gctcgaaccc gacgatcatg tcgtgtcacg cgcgcaagga
      481 cggcgaccat tacgtgatcg acggccacaa gtggttcacg accgccgccg acggcgctgc
      541 gttcgcgatc gtgatggcgg tgacgaaccc ggaggcgcca ccgcatgcgc gcgcgtcgat
      601 gatcatcgtg ccgaccgatt cgcccggctt cgagcgcgtg cgcaacatca agatcatggg
      661 cgaccccggc gaaggctggg gcagccacgc cgagatctgg taccgcaacg tccgcgtacc
      721 cgtcgagaat cgcctcggcc ccgagggcgc gggcttcatg atcgcgcagg agcgcctcgg
      781 ccccggccgt atccatcact gcatgcgctg gatcggcatc tgcgagcgcg tgttcgacac
      841 gatgtgcaag cacatcacgc gccgcaagat cgacgacgag aagacactcg cgacgcgcca
      901 gatcgcgcaa gcgtgggtcg ccgaagcgcg tgccgagatc gatgcatcgc gcctgatggt
      961 gctcaacgcg gcgtggacga tcgagcgcaa gggctttcag gccgcgcgcg atcaagtgtc
     1021 gttgatcaag ttctacgtcg ccgacgtgat gctgcggctc gtcgaccgcg cgatccagat
     1081 gcacggcgca ctcggcatca cgagcgacac ggtgctcgcg ttctattacg tgcacgagcg
     1141 cggcgcgcgc atctacgacg gcccagatga ggtccacaag atggtggtcg cgaaacgcat
     1201 cctcgcgagg tactcgtgat cggcgaagat caagcgaagc ccgtccgcga gggcgaagag
     1261 ctgccgctgc ccgcactcaa agcgttcctc gagagcgaag tcgggttgct cggccccgtt
     1321 cgcgtcgagc aatttcccgg tggacactcg aacctgacct atctcgtgca tcacggcgat
     1381 cgcgagtacg tgctgcggcg gccgccgttc ggcagcaagg tgaaatcagc gcacgacatg
     1441 ggtcgcgagg ttgcggtgct gtcgaagctc gcgccggtct acacgcgcgc accgcacgtg
     1501 atcgcatact gcgactcgcc tgacgtgatg ggcgcgccgt tctatctgat ggagcgccgg
     1561 cgcggcatca tcctgcgcaa ggacctgccg ccgaacctcg accacgcgcg catcgcgcac
     1621 ctgtgcgatg cactcgtcga cgcgctcgtc gatctgcacg cggtcgatta ccgcgccgcc
     1681 ggcctcggtg acttcggcaa gcccgccggt tacgtcgagc gacaggtcaa aggctggagc
     1741 gagcgctaca tcggctcgca gaccgacgac atccccgcga tgaccgaggt tgctgcgtgg
     1801 ctcgctacgc acatgccggc ggatggcccg ccgtcgctga tccacaacga cttcaagttc
     1861 gacaacgtga tcttcgatcc gtcactcgat cagatcgtcg gcgtgctcga ctgggagatg
     1921 gcgacgatcg gcgacccgct gatggatgtc ggcacgagcc tgtcgtactg gatgcaggcc
     1981 gacgacgccg cgatgttcca gagcccgatc ttcggcatca cgatgaagcc aggcatgctg
     2041 cggcgcgctg acatcgccgc gcgctacttc gaccgctccg gccgccagac cgatcacctg
     2101 gtcttctact tcgcgttcgg cctgttcaag accgccgtga tcttgcagca gatctattac
     2161 cgctacgcga agggcctcac acaggacgca cgctttgcgc cgttcatcat ggtcgttcgc
     2221 ggactcgcgg accacgcgcg cgcgtcgatc gaacgcatgt cgctctagct cccaccgcag
     2281 taatttgcgg cggtgagccg acggcctcgc cgctctgggc gatactcgtc gacatgcgat
     2341 gccgcggagc tctcacctgc atcttcgtgg ctggttgcgc gcttgcgaag ccaccgccac
     2401 ccgcgccgcc acggcatacc gagctcgggc tgtcggtcac gtcggtgacg ctcgcgaatg
     2461 ggctacgcgt cgtgctggtc aaagacccgc acgcgagcga ggtccaggtg acgatgcgct
     2521 atcgggttgg ctccgccgac gatctcgagc atccgggcat cgcacacctt gtcgagcacc
     2581 tgatgttcca gcaaacgctc ggctcgcaga cgttgttcgc gcacctcgaa gacaacgcga
     2641 cgtttttcaa cgctgtcacg acgttcgacg gcacgacgta catctctcgc gcccggcctg
     2701 acttcctgga caaactgctg tcgatcgaag ccgtgcggct cggctttagg tgcacgacga
     2761 tcaatgactc cgcattcgag cgcgagcgtg aggtcgtgat gcaggagctc acgctgaaga
     2821 gcgaagcgtt cgatctgtgg gacagcctgc tgcgcgccgt ctatcccgcg aatcacccct
     2881 atgcaggtgc acgcgacacc gtgaaaacag tctccgagat cacgcgtgcc gaggcgtgcg
     2941 cgttcgccga tgcctattac gcgccgagca acgcagcact cgtgatctcg gggaatctca
     3001 cgaacgagca ggtggagaca tcgctcggca agttcctcgc tcggatcgcc aagcgcgtcg
     3061 cgtcgtcacc ggcgccggtc gccgcggtta cacggctgct tccacacgtc gcgtcggctc
     3121 ccgtcgacag cagagtgctg ctcgtcacct ggccgttgcc actcgatccg ggcgatcaga
     3181 tcgcagtgcg cgcactgttc agccaagcag caattggcgt ggtgaacaat gcggtcaaag
     3241 gccacgtccg ggcgatcgag ctcggagacg tgcgtgcgcc ggtgatcggc gtcgccatcg
     3301 aggtgatgga aacggaatcc gtgcaggacg tgctcgatcg cgtccaggac gtgttcgttc
     3361 agctcccgaa tgagctcgag agctacatgc ccaacctcgc gttcgacgcg atccggcaac
     3421 gcacgatcta cgagcacttc gccgcgctcg aggacggcag cgagcgcgac agccgcctcg
     3481 cggtccacgc gctcgccggc cagaatcccg ctgacgcagt cgccgcggaa cagcgctcgc
     3541 tgcgcaacct cggtcgccac gaggtaggcg cgctcacccg gcgctacctc gcgttcgatg
     3601 acgcgacact ggtcacgctc gagccgacgg caagcaagaa gacaggccac gcgctcgacg
     3661 tgcggccggc aatccacgac atgggcctgc gccggagtac acccgaggtc gcgcgcgcga
     3721 tgacgccgga cgagtcgatc gcggtgccat cgatcactgc cacgacacgc gtgctgccga
     3781 acgggctcaa ggtcgtgctg ctgccgctca cctcagtgcc gacggtcgac atgcgactcg
     3841 tcttcggcgc aggggccgcc gacgagcccg tcgacaagcg cggcgccgcg ctcgtcgctg
     3901 gctacggcct gtcctccgac ttgcgcaacc tcaatgacac gatcaacttc tacgttgccg
     3961 gtggttcgat cgatgccgac gtcacggccg agcgcacgtc gttcgaggtc aaaggcgtcg
     4021 acatgcatct cgattacctg ctcgccggac ttcgccgctg ggtcgtcgac ggccgctact
     4081 cgagcagcgc ggacctcatg gtcgagctcg tcgagcgcgc acgcaagcgc aagaacgaaa
     4141 atggcgcatt gatcgacgcg tggcagacgg cggtctacgg cgagaacaat ccatacgcgc
     4201 gcgccggcat cgcgagctac gggac
//
LOCUS       DASUFX010000750        13406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_539059,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000750 DASUFX010000000
VERSION     DASUFX010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539059"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFV99_30310"
     CDS             complement(<1..469)
                     /locus_tag="VFV99_30310"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX5063725.1"
                     /translation="MSFDVVVAADLDWGIGKANGLPWPKLQGDMAHFRRITSACSEGK
                     VNAIIMGRKTWESNEMGGKPLPKRLNVVVTRQNLAVPEGVVVAATLDGALMAARGDNI
                     ENVFVIGGAELIRHSIGHADLRWIYLTRIEGHFQCEVAIPDLDAAGFAKVAWDG"
     gene            500..1495
                     /locus_tag="VFV99_30315"
     CDS             500..1495
                     /locus_tag="VFV99_30315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063726.1"
                     /translation="MRSAVALLAVAACGGGSNNAPDASMTASDGESGGMTLSDQYPGD
                     VGLGGDPAVIWFEDFEEGSVAAVTARYNQAQGMARMQLVTDAAHGSALALTAGGSVSA
                     VDLYKQLPDHDDVFVRWYVKYDAAIPWHHSGMWFGGYNPAMSYPMPGAGLKPDGDDRF
                     SISIEPVWDVGSAAARFDSYNYWMNMRSWMAVPTNDGTSYYGNSFVHENGFTIDETKW
                     VCLEVHVHVNTAGTSATGGVLEVWKNDALVRRFDDAGPMGWWVRDKFCPANTNASECT
                     DYPATPNEILDLQLRSTTSYAVNAFWPQNYITDPTMGTLRFDQMVVATKRVGCLR"
     gene            1607..2197
                     /locus_tag="VFV99_30320"
     CDS             1607..2197
                     /locus_tag="VFV99_30320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063727.1"
                     /translation="MRILVWGLLLAVPQVASAEPRWSFGWRSAISSDLTPPPWLICGG
                     QWRWRVVPGIALGIDGDGGRGDRYETHDALRTAAERTMAKAGASVSKYLAHGGPWEVR
                     VFGGGGLAVELTHVKVLDLDWVQDRIGYRPQAFAGVGVERLWPRTWEGTKPGAAGVAL
                     ELRGMWLAGDAPMSAAPELSRFSVQLALELNAYTGQ"
     gene            2213..2992
                     /locus_tag="VFV99_30325"
     CDS             2213..2992
                     /locus_tag="VFV99_30325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5063728.1"
                     /translation="MTRIAVVTGAGRGLGRCIAERLIKRGYGVLVTDIDGAAAQATAA
                     ALGDGAWALQQDVRDPDSHRAVARAATARGDVELWINNAGVLAVGETWQMDEADIRRM
                     VDVNVLGVMWGCHAAIPVMQKGQIINVSSMSGLTPSPGLAVYGASKHAVVGYSLSLAG
                     ELRRAGKAIEVSVLCPDAIRGDMTNAVAHDQAAGLIFSAGTMLSLDEVADAALELVDE
                     PKLIRTMPRYRAALVHLLRPFPALGLPLLEQFAKRGRRRAR"
     gene            complement(3013..4341)
                     /locus_tag="VFV99_30330"
     CDS             complement(3013..4341)
                     /locus_tag="VFV99_30330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5063729.1"
                     /translation="MYKGFVRLRGVYIKLGQILSIMGSFLPREYVEELEGLQDDVPPT
                     SYKDIAETFKKSFKKMPTEAYATFNKEPIAAASLGQVHEATNAAGERLAVKILYPNVA
                     TIIRVDLRVLGWALRVYKNFVPLQQIERVHEQLSDMLNRETDLVNEAAMIEKMAKNFE
                     SDPDVLFPKVYPELSSHDVMTMSFMDGVKITKKSALSKLELDPYAVATKLIKVFYKQL
                     FIDRFFHADPHPGNFFVQRGPEGQPRIVVLDLGSATELRANLADGMFDILSGLMTRKD
                     DLVVKGIDEMGFMAADGNRALLERTTRKYFEKLLNLNITDFGKIDPAVAQQLADPDMK
                     REELRELMKSVAYPEGWFYVERASVIMFGLSATLAPKLNGIQVGFPYVMQFMMERNAA
                     RLAQGSTAVATVPKTKVEAKPSEKAVEKPDKSEKADKPEKPDAKDEVLLN"
     gene            4529..5434
                     /gene="hemF"
                     /locus_tag="VFV99_30335"
     CDS             4529..5434
                     /gene="hemF"
                     /locus_tag="VFV99_30335"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX5063730.1"
                     /translation="MSDLFDRAAKFFHELQGDICRDLADVDGAREFGADAWQRPGGGG
                     GVARVLEGGALFEKAGVNWSNVDGELPAELADNMPGTGRQFRASGVSLVLHPQSPMVP
                     TTHANFRCIQKGDTVWFGGGADLTPYYFFREDAVHFHKTLAEACDAHTPIGDYDRFKA
                     WCDEYFFLPHRGETRGVGGVFFDYLGSKGEHPVEDIFRFVQELGRAFTKAYIPIAQRR
                     QTAPFGETERTWQLRRRGRYVEFNLIYDRGTLFGLKTNGRIESILMSLPPLVRWDYDV
                     MAQPGSPEAELLAHLRATDWLGRAR"
     gene            5585..6007
                     /locus_tag="VFV99_30340"
     CDS             5585..6007
                     /locus_tag="VFV99_30340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063731.1"
                     /translation="MAKKQTRRSISVSRTTYERLKAFCESNNISMSQFVETRVGDFLG
                     QGEGTTTIIPTTPRPTAPVPAKPATSTPGASGPKSETSAPRPVVPAAAPVAQPVPMTS
                     AAARYAVEPPPPVIKRRTEEEQAAPKPAEKRPEQIFTF"
     gene            6393..7319
                     /locus_tag="VFV99_30345"
     CDS             6393..7319
                     /locus_tag="VFV99_30345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5063732.1"
                     /translation="MSKSLLFVFVLAFAIGGCAKKHDNAPTPSGSAGSSAGNGSAGSS
                     AGGSGASDSAGSGSGSAGSGAGSGSAAEIPPYTPAADVPAPIRDAVTAADRSDDDRKL
                     DAGRKPAEVLAFFKLAPGQKIGELFAGGGYTSEVMARIVGDKGTIYAQNTKEVLDRFA
                     RKPWSERATKPVMKHVVAVERPTDDPFPAEAKKLDAVITILNYHDFVWQKADRAKLNK
                     AVFAALKKGGVYGIVDHSAAPGSGTRDCETLHRIDEEVVKQEVQAAGFKLDAESDLLR
                     RPDDKRDWNSSPKAAGEKRGTSDRFTLRFVKP"
     gene            7355..8659
                     /locus_tag="VFV99_30350"
     CDS             7355..8659
                     /locus_tag="VFV99_30350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5063733.1"
                     /translation="MAVRAPIRIARALQHRNYRLFFFGQGISLIGTWLTRFATVWMVY
                     RLTHSGALLGLVAFFGQAPSSVIAPIAGVLIDRWDRHRVVIATQVAAMLQSAALAFFA
                     FTGLMTVWHLMVLGAVQAVINAFDMPARQSFLGQMIEDRADLPNAIALNSSMVNGARL
                     VGPAIAAVLVGLFGEAWCFAVDAVSYVAVIVSLLMMHVPKRALVARRGHVWQDMQDGW
                     HYVANAPLVRAVLLLLAVTSVLGGAYTTLLPMVAGSTLQGGSNTLGILMATAGCGALA
                     GALYLAGRSTVVGLGGAIGRATLALGAALIVLRFATNVWIAAPLLFVTGAAWMIQMAA
                     TNTIIQTIVDTDKLGRVMSLYAVAFFAGMPVGALLEGWLADHLGPMNTFLCAGLAVSI
                     AGVMYMRALPRLRLVSRPLYERLGLISAPDAPPSREQLPAAH"
     gene            complement(8607..9665)
                     /locus_tag="VFV99_30355"
     CDS             complement(8607..9665)
                     /locus_tag="VFV99_30355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5063734.1"
                     /translation="MLLLDESGTRLHVAAAPGLPATYNKMLEGASIGPRAGSCGTAAY
                     YGQPVHVEDILTDPLWVDYRDLAKLSGMRGCWSTPIFSADQRVLGTFALYHKQPGLPD
                     RVALELIERATHVASLVIERRLLDEEQRALAARIEAVREEERTGIARELHDELGQALT
                     ALKLDVAWIARNTHENGSMHARLDEMAQLVDTTLQSVRRISSELRPVILDELGLGATI
                     QWQADEFGARTNIELNVENALPEQPIDRTVSTAAFRIFQEALTNIARHAEATRVDVRL
                     RASEGNLIMEVADDGVGLPELVRRGSPQSLGLVGMRERARRLGGDCIVMRRQPRGTLV
                     VLSVPLGAAPEMAAHPVH"
     gene            9601..10242
                     /locus_tag="VFV99_30360"
     CDS             9601..10242
                     /locus_tag="VFV99_30360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063735.1"
                     /translation="MAGNPGAAATCSLVPLSSSSSTVAVVAGSSLEIDSAQSRGRGIC
                     RGEQIRFRVVRITDAPRGECDLHFAHDLGVEADQMLFLAQLGDIGDGPDQLADVATGF
                     DDRRHRHLADLLAGRIVGSFFPAHRASLGNRLPVLLHHHRSRWFRKDFVHTQTDDLVL
                     GQPGRLGERAVDVDEPEPVAFHPQVKDDVVDVRVDQRLDAVADVSAAACHAES"
     gene            10619..10972
                     /gene="queF"
                     /locus_tag="VFV99_30365"
     CDS             10619..10972
                     /gene="queF"
                     /locus_tag="VFV99_30365"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5063736.1"
                     /translation="MTTRPSKTLDTFPNPRSGRDYHIAFECPEFTCLCPMTGQPDFAT
                     IFIDYVPDALCVELKSLKLYLWSYRDEGAFHEAVTNQICDDLVKAIAPRSITVTGKFW
                     VRGGITTTVTVTHAK"
     gene            complement(10985..11119)
                     /locus_tag="VFV99_30370"
     CDS             complement(10985..11119)
                     /locus_tag="VFV99_30370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063737.1"
                     /translation="MAAITRIPNLHTQAGARWLAPFLLIVLATLGVAYVMVVYPYVTR
                     "
     gene            complement(11178..11426)
                     /locus_tag="VFV99_30375"
     CDS             complement(11178..11426)
                     /locus_tag="VFV99_30375"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5063738.1"
                     /translation="MSTNTSTDDEIRRCEVCNAEIPAARVKAIPGTWLCIECSEDVGG
                     DFVYTAKQENLGKAGSLKLNYGGVSVTKKRRTIRKPGE"
     gene            complement(11532..12761)
                     /locus_tag="VFV99_30380"
     CDS             complement(11532..12761)
                     /locus_tag="VFV99_30380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5063739.1"
                     /translation="MAGEIAHPLIVPLPRLVAPAALALRHLALARLRAMFASADARLD
                     LELPNGETVELGSRGLYARARVHDDRLFLRVLLRGELGSGESFVAGEWSSDDVVGVVR
                     AFLRATNARGVESPLTRLLQLPALLRHRRAANTHVGSQRNVHAHYDLGNAFYRLFLDD
                     DTLAYSCAIFPAASLADAQRAKFDRLCDWLALSPRDHLLEIGCGWGGMAIHAARTRGC
                     RVTAITVSREQYELASARVAAAGLADRVTIEYRDYRGVDPQRTFDKIVSIEMLEQVGF
                     EYLPGFFATCARVLAPGGTLAVQTITMPDDRFDAYRRSVDWMQTYIFPGTLIPSLGAI
                     RAACPPFAITRSDDIGDSYVPTLRAWRDRFVAALPAVRALGFDPPFIRTWLLYLAFSE
                     AAFAERTLGDHQLLLTR"
     gene            complement(12787..13194)
                     /locus_tag="VFV99_30385"
     CDS             complement(12787..13194)
                     /locus_tag="VFV99_30385"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5063740.1"
                     /translation="MRERAREDAAIETNEQEAEEVPEQPSRTRFVGGAIPPVQVAQEH
                     EDPNVIELKQKVQRLVTSKFGGDYKKAFDHYDGDQDGKMTKDEIKQMLSDAGVGNGLT
                     RGAWADGILKKLDMNHDSGVQWGEFESVFKATA"
     gene            complement(13204..>13406)
                     /locus_tag="VFV99_30390"
     CDS             complement(13204..>13406)
                     /locus_tag="VFV99_30390"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEX5063741.1"
                     /translation="RLFTARLTGDRAPLSDRALLSAALRYPLMSAQVIGLIHFEALKM
                     RLAGVPYHRPEHDHRPLVRPAR"
BASE COUNT         2180 a         4309 c         4518 g         2399 t
ORIGIN      
        1 cgccgtccca cgcgaccttc gcgaagcccg cggcgtcgag atccggaatc gcgacctcgc
       61 actggaagtg gccctcgatg cgggtcagat agatccagcg cagatcggcg tgaccgatcg
      121 agtggcgaat cagctcggcg ccgccgatca cgaacacgtt ctcgatgttg tccccgcgcg
      181 ccgccatcag cgcgccgtcg agcgtggcgg cgacgacgac gccttccggc accgcgaggt
      241 tctggcgcgt gacgaccacg ttgagtcgct tcggcagcgg cttgccaccc atctcgttcg
      301 actcccacgt cttgcgaccc atgatgatcg cgttgacctt gccttcgctg cacgcgctcg
      361 tgatgcggcg aaagtgcgcc atgtcacctt gcagcttggg ccatggcaat ccgttcgctt
      421 ttccaatgcc ccaatcgagg tcggcggcaa cgacaacgtc aaagctcacg cacgcaccat
      481 agcgtgagat agtcgtcgtg tgaggtcggc agtcgctctg cttgcggtcg cggcgtgcgg
      541 tggcggtagt aacaacgctc ccgacgcgag catgacagcg agcgacggcg agagcggcgg
      601 catgacgctg tcggatcagt atccgggcga tgtcgggctc ggtggcgatc cggcggtgat
      661 ctggttcgag gatttcgaag agggctcggt tgcggccgtg acagctcgct acaaccaggc
      721 gcagggcatg gcgcgcatgc agctcgtcac cgatgcggcg catggcagcg cgctcgcact
      781 cacggcgggt ggcagcgtgt cggctgtcga tctgtacaag cagctgcccg atcacgatga
      841 cgtgttcgtg cgctggtacg tgaagtacga cgccgcgatc ccgtggcatc actccggcat
      901 gtggttcggc gggtacaacc cggcgatgtc gtatccgatg ccgggcgcgg ggctgaagcc
      961 cgacggcgac gatcggttct cgatctcgat cgagccggtg tgggacgtcg gcagcgcagc
     1021 ggcgcggttc gactcttaca actactggat gaacatgcgc tcgtggatgg cggtgccgac
     1081 caacgacggc acgtcgtact acggcaactc gttcgttcac gagaacggct tcacgatcga
     1141 cgagacaaag tgggtatgtc tcgaggttca cgtgcacgtg aacaccgctg ggacatcggc
     1201 gacgggcggc gtgctcgagg tgtggaagaa cgacgcgctg gtccgccgtt tcgacgatgc
     1261 cggcccgatg ggctggtggg tgcgcgacaa gttctgcccg gcgaacacga atgcgagcga
     1321 gtgcaccgac tacccggcga cgcccaacga gatcctcgat ctgcagctgc gcagcacgac
     1381 gagctacgcg gtcaacgcgt tctggccgca gaactacatc accgacccga cgatgggcac
     1441 gctgcgcttc gatcagatgg tcgtcgcgac gaaacgcgtc ggctgtttgc gctaatcgtg
     1501 tagcgtacgc gcggcgacac cgtgtgaaga cgcggtcacg cttgcggcga aaccgcgcga
     1561 acgaacgcgc attcgtgcgg gcacgcgcgt tgctgatggc gggcgcatgc gcattctcgt
     1621 ctgggggctg ctgctcgcgg tgccacaggt tgccagcgcc gagccgcggt ggtcgttcgg
     1681 ctggcggtcg gcgatcagta gcgatctcac gccgccgccg tggctgatct gcggtgggca
     1741 gtggcggtgg cgcgtcgtgc caggcatcgc gctcggcatc gatggtgatg gtggtcgtgg
     1801 cgatcgctac gagacacacg acgcgctgcg caccgcggcg gagcgcacga tggcgaaggc
     1861 aggtgcgagc gtctcgaagt acctcgctca cggcggcccg tgggaggtgc gcgtgttcgg
     1921 cggtggtggc ctcgccgtcg agctcacgca cgtgaaggtg ctcgacctcg actgggtgca
     1981 ggaccgcatc gggtatcggc cgcaggcgtt cgccggcgtc ggcgtcgagc ggttgtggcc
     2041 gcggacatgg gaaggcacga aaccgggtgc agcgggcgtc gcgctcgagc tgcgcgggat
     2101 gtggctcgcg ggtgatgcgc cgatgagcgc ggctcccgag ctatcgcgct tcagcgtgca
     2161 actcgcgctc gagctcaacg cctacaccgg ccagtagcgt ataacgcgcg cgatgacgcg
     2221 catcgcagtg gtcactggag caggacgggg ccttggccgt tgcatcgccg agcgcttgat
     2281 caagcgcggc tacggcgtgc tcgtcaccga catcgatggt gccgctgcgc aggcaaccgc
     2341 cgccgcgctc ggtgacggtg cgtgggcgct ccagcaggac gtgcgcgatc cggactcgca
     2401 tcgcgcggtc gcgcgtgctg ccaccgcgcg cggcgacgtc gagctgtgga tcaacaacgc
     2461 gggcgtgctc gcggtcggcg agacctggca gatggacgag gcggacatcc ggcgcatggt
     2521 cgacgtgaac gtgctcggcg tgatgtgggg ctgccacgcc gcgatcccgg tgatgcagaa
     2581 gggccagatc atcaacgtct cgtcgatgtc ggggctcaca ccgtctcccg gcctcgcggt
     2641 ctatggcgcg tcgaagcacg cggtcgtcgg ttattcgctg tcgctcgccg gcgagctgcg
     2701 ccgtgctggc aaggcgatcg aggtcagcgt gctgtgcccc gatgcgatcc gcggcgacat
     2761 gaccaacgcg gtcgcgcacg atcaggcggc cgggctgatc ttctcggcag gcacgatgct
     2821 gtcgctcgac gaggtcgccg atgccgcgct cgagctcgtc gacgagccga agctgatccg
     2881 cacgatgccg cgctaccgtg ccgcgctcgt gcacctgttg cggccgttcc ccgcgctcgg
     2941 cctaccgctg ctcgaacaat tcgcgaagcg cggccgccga cgcgcgcggt aggctcggca
     3001 cgaatcggct cattagttga gcagcacttc gtctttggcg tcgggcttct cgggcttatc
     3061 ggccttctcg ctcttgtctg gcttttcgac ggccttctcg ctcggcttcg cctcgacctt
     3121 cgtcttcggc acggtcgcca ctgccgtcga accttgcgcc agtcgcgcgg cgttgcgctc
     3181 catcatgaac tgcatcacgt acgggaagcc gacctggatg ccgttgagct tcggcgccag
     3241 cgtcgcggac aggccgaaca tgatcacgct cgcgcgctcg acgtagaacc agccttcggg
     3301 atacgcgact gacttcatca gctcgcgtag ctcctcgcgc ttcatgtccg gatcagcgag
     3361 ctgctgggcg accgcaggat cgatcttgcc gaagtcggtg atgttgaggt tgagcagctt
     3421 ctcgaagtat ttgcgcgtcg tgcgctcgag cagcgcgcgg ttgccgtcgg ctgccatgaa
     3481 gcccatctcg tcgatgccct tcacgacgag gtcgtccttg cgcgtcatca gacccgacag
     3541 gatgtcgaac atgccgtcgg cgaggttcgc gcgcagctcg gtcgcgctgc cgagatcgag
     3601 cacgacgatc cgcggctgac cctcggggcc gcgctgcacg aagaagtttc cgggatgcgg
     3661 atcggcgtgg aagaagcgat cgatgaatag ctgcttgtag aagactttga tcagcttcgt
     3721 cgcgaccgcg tacggatcga gctcgagctt ggacagcgcg ctcttcttcg tgatcttgac
     3781 gccgtccatg aacgacatcg tcatcacgtc gtgcgagctg agctccgggt agaccttggg
     3841 gaacaagacg tccggatcgc tctcgaagtt cttcgccatc ttctcgatca tcgcggcctc
     3901 gttcacgagg tcggtctcgc gattgagcat gtcggagagc tgctcgtgca cgcgctcgat
     3961 ctgctgcagc gggacgaagt tcttgtagac gcgcaacgcc cagccgagca cgcggaggtc
     4021 gacccggatg atcgtcgcga cgttcgggta caggatcttg accgcgagcc gctcgccggc
     4081 tgcgttcgtc gcttcgtgca cctggccgag cgatgccgcc gcgatcggtt ccttgttgaa
     4141 cgtcgcgtag gcctcggtcg gcatcttctt gaacgatttc ttgaacgtct cggcgatgtc
     4201 cttgtacgag gtcggcggga cgtcgtcctg caggccttcg agctcctcga cgtactcgcg
     4261 gggcaagaac gagcccatga tcgacaagat ctggccgagc ttgatgtaca cgccgcgcag
     4321 gcggacgaag cccttgtaca tgcgccgcgc gttgcggcga ttgacccgct tccaatgggc
     4381 gctggtgcca taccgtgagg gccacaaccg cgcccacgac atctgccaca agtaactgag
     4441 gaagataatc gcgaacacgc gcgtcgcacg gattaagcga atcgcggccc acatctccta
     4501 gcgctctcga cgatggtagc atgcgcgcgt gtcggacctc ttcgatcgcg ccgcgaagtt
     4561 ctttcacgag cttcagggcg acatttgtcg cgatcttgcc gatgtcgacg gcgccagaga
     4621 atttggcgct gatgcatggc agcggccagg tggcggcggc ggtgtggcac gcgtgctcga
     4681 gggtggcgcg ttgttcgaga aggccggcgt caactggtcc aacgtcgacg gtgagcttcc
     4741 cgccgagctg gcagacaaca tgccaggcac gggccgccag tttcgcgcga gcggcgtatc
     4801 gctcgtgctc catccgcaat cgccgatggt gccgaccacg catgcgaatt ttcgctgcat
     4861 ccagaagggc gacacggtgt ggttcggcgg aggcgctgac ctgacgccgt actacttctt
     4921 tcgcgaggac gcggttcact ttcacaaaac gcttgcggag gcgtgcgacg cccacacgcc
     4981 gatcggtgat tacgaccgat tcaaggcgtg gtgtgacgaa tacttctttc tcccgcatcg
     5041 cggcgagacg cgcggcgtcg gcggcgtgtt cttcgactat ctcggcagca agggcgagca
     5101 tcccgtcgag gacatcttcc ggttcgtgca ggagctcggt cgtgcgttca cgaaggcgta
     5161 catcccgatc gcgcagcgac ggcaaaccgc gccgttcggc gaaaccgagc gcacgtggca
     5221 gctgcgccgg cgcggccgct acgtcgagtt caatctcatc tatgaccgcg gcacgctgtt
     5281 cggtctcaag accaacggcc gtatcgagtc gatcctgatg tcgctgcctc cgctcgtccg
     5341 ctgggactac gacgtgatgg cgcagccggg atcgcccgag gccgagctgc tcgcacatct
     5401 gcgcgcgacc gactggctgg gccgcgcccg gtaagcgcca gaaaaggggt cagaccccat
     5461 ttcttggcgc gcccgagatc cgtccgatgt cagacggctc tagtagggtc ctgcacgtcc
     5521 acgggagtac aaggctagta ggttcgtctt gacgaccccc tacctgtagc gtaaggaaat
     5581 tactatggcc aagaagcaga ctcggcgcag catcagtgtg agccgcacga cttacgagcg
     5641 actgaaggcg ttctgcgagt cgaacaacat ctcgatgagc cagttcgtcg agacccgcgt
     5701 cggggacttc ctcgggcagg gcgagggtac gacgacgatc atcccgacga cgccgcggcc
     5761 tacggcaccg gtgccggcga agcctgcgac ttcgactcct ggagcttcgg ggccgaagtc
     5821 ggagaccagc gcgccgcgtc cggtcgtgcc ggccgctgcc ccggtggcgc agccggttcc
     5881 gatgacgtcg gccgctgctc gctacgcggt cgagccgccc ccgccggtga tcaagcgccg
     5941 gaccgaggaa gagcaggccg cgccgaagcc ggccgagaag cgcccggagc agatcttcac
     6001 gttctagcgc ctcgctggtg gtcgtgcttc gcacgactcg cgccctgcgg tcgcttccgc
     6061 cgcttcgcgg cgcaccagct cgcgctagac ccgcgcgggc aacgcggctc cgccgcttcc
     6121 cgcggggttc tgatgtgcgc ttgcggtcgt ggttcgcacg actggcggct ttgccgcttc
     6181 cgccgcttcg cggcgcgcaa gcttcatcag acccgcgcgg gcaacgcggc tccggcgctt
     6241 cccgcggggt tctagcgcct cgctggtggt cgtgccttgc acgactcgcg gttccagtcg
     6301 tgttgggcgc ggctaatcac cgcggaacac atcgtcgctc gtacatcgct agtcgcgtgg
     6361 aatctccatc gactagcgtc ggttcagcga ccatgtctaa gtctctcctc ttcgttttcg
     6421 tcctcgcgtt cgccatcggc ggatgcgcca agaagcacga caacgcgccc acgccgagtg
     6481 gttccgccgg cagctccgct gggaacggtt cggcaggcag ctcagcgggc ggctcgggag
     6541 cgagcgattc ggcggggtcg ggcagcggtt cggcgggaag cggggcaggg agcggttcgg
     6601 cggcggagat tccgccgtac acgcctgccg ccgatgttcc ggcgccgatt cgcgacgcgg
     6661 tgacggcggc cgatcgcagc gacgatgatc gcaagctcga tgccggacgc aagccggccg
     6721 aggtgctcgc gtttttcaag ctcgcgcccg gacagaagat cggtgagctg tttgccggcg
     6781 gcggctacac gtccgaggtg atggcgcgca tcgtcggcga caaggggacg atctacgcgc
     6841 agaacaccaa agaggtcctc gacaggttcg cgcgcaagcc gtggagcgag cgcgcaacga
     6901 agccggtgat gaagcacgtc gtcgccgtcg agcgtccgac cgacgatccg tttcccgccg
     6961 aggcgaagaa gctcgacgcg gtgatcacga tcttgaacta tcacgacttc gtctggcaga
     7021 aggccgatcg cgcgaagctc aacaaggcgg tgttcgctgc gctcaagaag ggcggcgtgt
     7081 acggcatcgt cgatcacagc gcggcgcccg gatcgggcac ccgcgactgc gagacgctgc
     7141 atcgcatcga cgaggaagtc gtgaagcaag aagtgcaggc tgcgggcttc aagctcgacg
     7201 ccgagagcga tttgctgcgg cgtccggacg acaagcgcga ctggaacagc tcgccgaagg
     7261 cagccgggga gaagcgcgga acgagcgatc gcttcacgtt gcgtttcgtg aagccgtaag
     7321 ggcgttcggc gcatcgaacg ctagactgga gatcatggct gtacgcgcgc caattcggat
     7381 tgctcgagcg ctgcagcacc gcaactaccg gctgttcttc ttcggccaag gcatttcgct
     7441 gatcggaacc tggctgacgc ggtttgcgac cgtgtggatg gtctatcggc tgacgcactc
     7501 gggcgcgctg ctcggcctcg tcgcgttctt cggccaggcg ccgagctcgg tgatcgcgcc
     7561 gattgcggga gtgctgatcg atcgctggga tcgccatcgc gtcgtcatcg cgacgcaggt
     7621 cgccgcgatg ctgcagtccg cggcgctcgc gttcttcgcg ttcaccggcc tgatgaccgt
     7681 gtggcacttg atggtgctcg gcgccgtgca ggcagtgatc aacgcgttcg acatgcctgc
     7741 gcgccaatcg ttccttggcc agatgatcga agaccgcgcc gatctgccaa atgcgatcgc
     7801 gctgaactcg tcgatggtca acggcgcgcg gctcgtcggt ccggcaatcg ccgccgtgct
     7861 cgtcggtcta ttcggcgagg cctggtgctt cgccgtcgat gcggtgagct acgtcgccgt
     7921 catcgtgtcg ctgctgatga tgcacgtgcc caaacgcgcg ctcgtcgcac ggcgcggcca
     7981 cgtctggcag gacatgcagg acggttggca ttacgtcgca aacgcgccgc tcgtgcgcgc
     8041 cgtgctgctg ctgctcgcgg tgacgagcgt gctcggtggc gcgtacacga cgctgttgcc
     8101 gatggtcgcc ggctcgacgc tgcaaggtgg ctcgaacacg ctcggcatct tgatggcgac
     8161 cgccggatgc ggcgcgctcg ccggtgcgct ttacctcgcg ggccgctcga cggtagtggg
     8221 gctcggcggc gcgatcggcc gcgcgacact cgcgctcggc gccgcgctga tcgtgctgcg
     8281 gtttgcaacg aatgtctgga tcgcagcgcc gctcttgttc gtgaccggcg cagcatggat
     8341 gatccagatg gccgcgacga acacgatcat ccagacgatc gtcgacaccg acaagctcgg
     8401 ccgcgtgatg agcttgtacg cggtcgcgtt cttcgctggc atgccggtcg gcgcgctgct
     8461 cgagggctgg ctcgccgatc atctcgggcc gatgaacacg ttcttgtgcg ccggtctcgc
     8521 ggtgtcgatt gctggtgtca tgtacatgcg cgcgctgcct cggttgcgac tcgtctcgcg
     8581 accgctctac gagcgactgg gcctgatcag tgcaccggat gcgccgccat ctcgggagca
     8641 gctcccagcg gcacactgag cacgacgagc gtgcctcgcg gctggcgccg catcacgatg
     8701 caatcgccac cgagtctgcg cgcgcgttcg cgcatgccga cgaggccgag cgactgcggc
     8761 gagccgcgac gaaccagctc gggaagcccg acgccgtcgt cggcgacctc catgatcaga
     8821 tttccttcgc ttgcgcgcag ccgcacgtcg acgcgggttg cttcggcatg gcgcgcgata
     8881 ttcgtcagcg cttcctggaa aatccggaat gccgcggtcg atacggtgcg gtcgatcggc
     8941 tgctcgggca gcgcgttctc gacgttgagc tcgatgttcg tacgtgcacc gaactcgtcg
     9001 gcttgccatt ggatcgtcgc gccgaggccg agctcgtcga gaatgaccgg ccgcagctcc
     9061 gacgagatcc ggcgcacgga ctgcagtgtc gtgtcgacca gctgcgccat ctcgtcgagg
     9121 cgcgcgtgca tcgatccgtt ctcgtgcgtg ttgcgggcga tccacgcgac gtcgagcttg
     9181 agtgccgtca gcgcctgacc gagctcgtcg tggagctcgc gtgcgatgcc ggtgcgttcc
     9241 tcttcacgca cggcctcgat gcgggcggcg agcgcacgct gctcctcgtc gagcagacgg
     9301 cgctcgatca ccaggctcgc gacgtgtgtc gcgcgctcga tgagctcgag cgcgacgcga
     9361 tcgggaaggc cgggctgttt gtggtagagc gcgaacgtgc cgagcacgcg ctggtcggca
     9421 gagaagatcg gcgtcgacca gcacccgcgc atgccgctga gcttcgcgag gtcgcggtag
     9481 tcgacccaga gcggatcggt caggatgtct tccacgtgga ccggctggcc gtaataggcg
     9541 gcggttccgc acgagcccgc ccgcggaccg atcgacgcac cctcgagcat cttgttgtag
     9601 gtggccggca atccgggcgc ggcggcgacg tgcagtctcg tgccgctctc gtcgagtagc
     9661 agcaccgtcg cggtcgtcgc ggggagcagc ctcgagatcg actccgccca gtcgcgaggc
     9721 cgcgggattt gccgcggcga gcagatacgt ttccgggttg tccggatcac cgatgcgcca
     9781 cgcggtgagt gcgatctgca cttcgcgcat gacctcggcg tagaggctga tcagatgctg
     9841 tttctcgcgc agctcggtga catcggagac ggtcccgatc agctcgcgga tgtggccacc
     9901 gggttcgacg accggcgtca ccgacacctc gccgatcttc ttgccggtcg gatagtgggt
     9961 agcttcttcc cagcgcaccg tgcgtcgctc ggcaatcgcc tgccggtact tctccaccac
    10021 catcgttcgc gatggttcag gaaagacttc gtccacacac agaccgacga cttggttctc
    10081 ggacagcccg gtcgcctcgg tgaacgcgcg gttgacgtgg atgaaccgga accggtcgcc
    10141 ttccacccgc aggtgaaaga tgacgtcgtt gacgttcgtg tagaccagcg cttggacgcg
    10201 gtcgctgatg tgtccgccgc cgcctgccac gcagaaagct agcacccgaa aaataggacg
    10261 ggcccggctt tcgcctgggc ccgtttgctc aacgtaccag aactaccgca gtagtcctgg
    10321 gtccgggagg cttacgaacg aacctcctgt gagcgggtac agtgacgttt catgtctttc
    10381 ttcctcccct ggcatagagc cagagcctga cggcacggcc cgcctgcact attgcagcgg
    10441 ctcccggcat caggccttca taatgggcac tacggcccgt ctcgaggccc acagcacgcg
    10501 gtgtccaaat ggccatcgct cgtgtactcg gcggccagaa ccggccgcac tttcagtcta
    10561 ggccaagtcg ggatctagcg ccagcatccg gatggcttta cgtgctaaga ggcgcgacgt
    10621 gacgacgcga ccgagcaaaa cgctggacac attcccgaat ccgcgatccg gacgcgacta
    10681 tcacatcgcg ttcgagtgcc cggaattcac gtgtctttgc ccgatgaccg gccagcccga
    10741 tttcgccacg atcttcatcg actacgtgcc ggacgcactc tgtgtcgagc tcaagagcct
    10801 gaagctctac ttatggagct accgcgatga aggcgcgttt cacgaagctg taacgaatca
    10861 gatctgcgac gacctcgtga aggcaatcgc accgcgatcg atcacggtaa ccggcaaatt
    10921 ctgggtgcgc ggcggtatca ccacgacggt gaccgtcacg catgcgaagt gatcgcgagc
    10981 tgattcagcg cgtcacgtac ggatagacga ccatcacgta cgcgacgccg agcgtcgcga
    11041 gcacgatcag cagaaacggc gcgagccagc gggctccggc ctgggtgtgg agattcggaa
    11101 ttcgagtgat tgccgccatg cccgatcgga gtgcatcgct cgcgcccgct ccgtcggaaa
    11161 agctcgcatg acgcgcctca ctctcccggt ttgcgaatcg tgcggcgctt cttcgtgaca
    11221 ctcacgccgc cgtagttgag cttgaggctg cctgccttgc cgaggttctc ctgcttggcg
    11281 gtgtagacga agtcgccgcc gacgtcctcg ctgcactcga tgcacagcca ggtgccgggg
    11341 atcgctttca cacgtgcggc aggaatctcc gcgttacaga cctcgcagcg tcggatttcg
    11401 tcgtcagtgc tcgtgttcgt gctcatggcg tagctctccc agtgcaagcg ggttccggcg
    11461 cttgcggctg cattatgagg atcggccgca cgaagtcgag gacgacctgg aagcgcgaat
    11521 gattccgcgg ttcagcgcgt gagcaagagc tggtgatcgc ccagcgtgcg ctcggcaaac
    11581 gcggcctcgc tgaacgcgag gtagagcagc caggtgcgaa tgaacggtgg gtcgaagccg
    11641 agcgcgcgga ccgcgggcag cgcggcgacg aagcggtcgc gccacgcgcg cagcgtcggt
    11701 acgtagctgt caccgatgtc gtcgctgcgc gtgatcgcga acggtgggca tgcggcgcgg
    11761 atcgcaccga gcgacggaat cagcgtgccc gggaagatgt acgtctgcat ccagtcgacg
    11821 ctgcggcgat acgcgtcgaa ccgatcgtcg ggcatcgtga tcgtctgcac cgcaagcgtg
    11881 ccgccgggcg cgagcacacg cgcgcaggtc gcgaagaagc cgggcaggta ttcgaagccg
    11941 acctgctcca gcatctcgat cgaaacgatc ttgtcgaacg tgcgctgcgg atcgaccccg
    12001 cggtagtcgc gatattcgat cgtcacgcga tcggcgaggc ccgcagcggc gacacgagcg
    12061 ctcgcgagct cgtactgttc gcgagacacg gtgatcgccg tgacgcgaca cccgcgcgtg
    12121 cgcgccgcgt ggatcgccat gccgccccag ccgcagccga tctcgagcag gtgatcgcgc
    12181 ggcgacagcg cgagccagtc gcacagccga tcgaatttcg cgcgttgcgc atcggcgagc
    12241 gatgcggctg gaaagatcgc gcacgagtac gcgagcgtgt cgtcgtcgag gaacaggcga
    12301 tagaacgcgt tgccgagatc gtagtgcgcg tgcacgttgc gctggctgcc gacgtgcgtg
    12361 ttcgcggcgc ggcgatgacg cagcagcgcg ggtagctgca gcaggcgcgt gagtggagac
    12421 tcgaccccgc gcgcgttcgt cgcacgcagg aacgcgcgga cgacgccgac gacgtcgtcg
    12481 ctcgaccact caccggcgac gaaggattcg ccggagccga gctcgccgcg caggagcacg
    12541 cgcaggaaca ggcgatcatc gtggacgcgc gcgcgggcgt agaggccgcg cgaaccgagc
    12601 tcgacggtct cgccgttcgg caactcgaga tcgagccggg catcagcgct cgcgaacatg
    12661 gcgcgcagcc gggcgagtgc aagatggcgt agcgcgagtg ccgccggcgc gacgagtcgc
    12721 ggcagcggaa cgatcaatgg atgtgcgatc tcgccggcca cccggtgcgc acgttacccc
    12781 ttttcgctac gcagtggctt tgaacaccga ctcgaactcg ccccactgca cgccgctgtc
    12841 gtggttcatg tcgagcttct tcaagatgcc gtcggcccac gcgccacgcg tcagcccgtt
    12901 gccgacgcca gcgtctgaca gcatctgctt gatctcgtcc ttcgtcatct tgccgtcttg
    12961 gtcaccgtcg tagtgatcga acgccttctt gtagtcgccg ccgaacttgc tcgtgacgag
    13021 ccgttggacc ttctgcttca gctcgatgac gtttgggtct tcgtgttctt gcgcgacctg
    13081 cacgggcgga atcgcaccgc cgacgaagcg cgtgcgcgac ggctgctccg ggacttcctc
    13141 ggcctcttgc tcgttcgtct cgatcgccgc gtcttctcgg gcacgctcgc gcatggcgat
    13201 aggctagcgc gccggccgaa cgagcgggcg atgatcgtgc tcgggccggt ggtacggcac
    13261 accggcgagc cgcatcttca atgcctcgaa gtgaatcagc ccgatcacct gtgccgacat
    13321 caacggataa cgcagcgcgg ccgacagcag cgcgcggtcg gatagcggtg cgcggtcgcc
    13381 ggtgagccgt gcagtgaaca gccgct
//
LOCUS       DASUFX010000751        10489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1232224, whole genome shotgun sequence.
ACCESSION   DASUFX010000751 DASUFX010000000
VERSION     DASUFX010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232224"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFV99_30395"
     CDS             complement(<1..884)
                     /locus_tag="VFV99_30395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063742.1"
                     /translation="MAQIYLARQTGLGAFERHVVLKTILRERATDQRFVTMFLDEAKL
                     AATLNHQNIAQVYEVDQADGAYFMAMEYVHGENARAILETTLRRGWTIPLELAVMIIS
                     GAAAGLHHAHERKGKNGAPLNIVHRDVSPANIMVGYDGSVKVLDFGIAKAEERATKTV
                     GGTIKGKYGYMSPEQCKGKPIDRRSDIFALGICLYELTTLRRAFKGNDDFETMKRIVA
                     GDVMLPSVAVPGYPRELEAIVLTAMANDPSARFQSGQEMIEALDAFAVRAKLTGSNTA
                     MGRFMVQLFGSKKEPWVEA"
     gene            complement(1037..1894)
                     /locus_tag="VFV99_30400"
     CDS             complement(1037..1894)
                     /locus_tag="VFV99_30400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063743.1"
                     /translation="MRYALVTLLIALVASRADAGRGTASLKYLPDDTNVVVVADVARA
                     RNSPIFKKLFTIAREQNTTLDTLASAQPIEKQVDTIVAASTPNKDAVIVLEGRIDKLV
                     AEAKKQATKTDTHAGVTFWVTADGEFTVVDKKLVFASAGQISGVIDRAKDKKAKGPAA
                     VRTIMAATTPNTAVFGGATLDASMKEQFNKSLGAEPQWAAVSFAMAQKLTIDARLKFA
                     DETAAATAVKTVNDQLTPDRRGQLEGFVGKDFADSVTIDQQQSFARIAAALTADELDK
                     VISFLKMVL"
     gene            1968..2738
                     /locus_tag="VFV99_30405"
     CDS             1968..2738
                     /locus_tag="VFV99_30405"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX5063744.1"
                     /translation="MGTANWPIALSGAIPALVAMWFVDRMDRKRPEPVRTRRLVALAG
                     MLSVIPALILEIAVGNTLGERVGPAMTYQGATFKAFIVAAAIEEACKIAVVYWVVWRR
                     PEFDERMDGIVYASRAGLGFALVENIFYLLQQGTLGGQLQTWILRAVLAVPGHAMWTG
                     MIGAMAARRRFDGKGLGLIGGYLLAVSFHGAYDLCAFLQQPLHLEGYDEYAPGLMPAL
                     IGLTLLAFFVLRSQARMALKLDDADAALNAAHAARTTQ"
     gene            complement(2704..3168)
                     /locus_tag="VFV99_30410"
     CDS             complement(2704..3168)
                     /locus_tag="VFV99_30410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063745.1"
                     /translation="MDSRSRWFTVGLALAAATSFAIAVQTAWWGIAEVAIGPFGSRHC
                     FGGECREAGLAWTGGSELWMRSAVATRAAGYIAMFVLIMFAGAIAAKREPKIVARGCL
                     AAIVTAIATGAYFYVGYPGVGGASVAAGFLFYVGAIVAGVAAIVLFLRRARR"
     gene            3387..3980
                     /locus_tag="VFV99_30415"
     CDS             3387..3980
                     /locus_tag="VFV99_30415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5063746.1"
                     /translation="MDRVLAEAEEEIPDLTMLTTEPVERTESSGYGWREDPINKRAKF
                     HHGSDYRGKRGTPVLAAGKGTVIFAGRRGGYGRVVFIDHGGGVITRYAHLDRIDVKVG
                     DALLAGMQLGKLGSSGRTTGAHLHFEVRLDGRDVDPNTAMIVADIMRENPAAGRLAAF
                     ALAPELQEHSRDAESPDIRVPSRPERAGRTKRQQVLW"
     gene            4010..6475
                     /locus_tag="VFV99_30420"
     CDS             4010..6475
                     /locus_tag="VFV99_30420"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5063747.1"
                     /translation="MVARSPDLLARRYVAWLRRRASAVIAAHVVLLALSVYLIAFRLP
                     LFADFSYLLPQDVPAVKDLRKLEARVKSTDSVLVLVTAPTPAAREAATQEVIDGLAAL
                     HSPLIARISGDEIAIREFLQARRHLFVPLDELEQARDTLQKKLDDEKLHANPLFIELD
                     DKDPNQAAADQKQLDDLRKKRQDAEAKLARPTNVCADGLTARVDVSTSFRTTDAKSGA
                     ELLAQLTGVRAQVIAKHPGVKIGFTGSPITALSEHHSITNGIVLSSLVTAGLVALVLA
                     LFFRSATLLVLLVSTIAIATAAAFGSAAFTVGHLNAATAFLGAIIAGNGINYGILLIA
                     RYLEERRRADVDEALAHAIVGTLRPTAVASLGAAIAYGSLAATSFKGFADFAIIGATG
                     MMLCWVASYVLLPALMLEFGRDTKIFQGNPRIGSALVRLLGFKRSGVVIAVASVVFAL
                     ATVVVVRYVAADPFEYDIRNLRSEASDAIEARQWMKISDEAFGRGYSGRTYIAADRLD
                     QVPMIIDALRTLDTGKPEAQRTIGAIESVLTFVPQDQDKRIAVLDEIRSLIDGAFDAM
                     DDKERAELGELRPPEGLHPFTANELPPTLRDKLTEKDGRIGYLIAIRPAPLLDEWNGK
                     DLIRFASAVRELHLRDNETVTTSGSSVIFADIVESIERDGPLVTGVALLGLIVMVVLL
                     VGRNRRAVAVLVGTVGGSLLMIAVCALLDLKVNFLDFVALPITLGLGIDYAINVAHRH
                     DTEELPDPITTLKTSGSAVFVCSLTTMIGYGSLLVSANLAIRGFGTASLIGEVTTVMT
                     ALVVVPALLAVGRKTSVQSIRSR"
     gene            complement(6504..7391)
                     /locus_tag="VFV99_30425"
     CDS             complement(6504..7391)
                     /locus_tag="VFV99_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5063748.1"
                     /translation="MLPTLEQLRTIAVIGAGTMGHGIAQVAAQMGAQTRLYDALDGAA
                     RAGVDRIAKNLAKGVELGKVSAADRDATMARISTRDALAATVEGVDCVIEAIPERIEL
                     KRETFAVIDEAAPAHALLATNTSSLPISDIAEAVRDGSRLVGMHFFNPVHVMKLVEIV
                     RHGKASDAAVALAVALAEKMGKTPITVKDSPGFASSRLGIVIGLEAIRMVEQGVASAA
                     DIDTAMKLGYGHPMGPLELTDLVGLDVRLGIAEYLASAIGPSFDPPQLLRNKVAAGQL
                     GKKTGVGFYRWSADGKRVG"
     gene            7441..8568
                     /locus_tag="VFV99_30430"
     CDS             7441..8568
                     /locus_tag="VFV99_30430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063749.1"
                     /translation="MLARWTCLAAALTGCSFSHGVAVDPTIEDGHIDDIDAPPADARP
                     DTPPGATCYGTGLFVDCYEPGTQPTNSDTLSSGMIDTSNASNCTRTVPQSNGPEICLR
                     TAVAITVNGYVRFKGSRPLVLLATSSITITTAGTLDVSSFRNDVGAGGDTGPCANVGN
                     GSADLSPSTNAGAGGAGAGGFGTPGGTGGSSNAGGGGGGGTTALTMIRGGCAGGKGGT
                     SNFGAGGDGGHGGGGVYLIAGSSITVVGTIKANGSGGNGGIYKSGGGGAGSGGLVAFD
                     APTLTITGTVYANGGGGGEGGGDNRTGENGSDPSAYNQRAGGGNSRLSGGDGGSGSVF
                     ANSGAGGGTANNGAGGGGGGAGRIKVYQASVPTGKLSPPAN"
     gene            8596..10236
                     /locus_tag="VFV99_30435"
     CDS             8596..10236
                     /locus_tag="VFV99_30435"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5063750.1"
                     /translation="MQPAVDVDRLQRELESIFGPRRVSLRAVDLDTYARDMWPRLLIA
                     YRDGIPPPHRPHAVVWPESVREVVAAVRLARELRLPIVPYGGGSGVCGGAVPLYGGIT
                     IDTKRMQAVRAVQTDELVCDVEAGLSGERFERELERRGYTFGHFPSSIYCSTVGGWLA
                     CRAAGQLSTKYGKVEDRVAGLTVVTGRGDVIETDGPNRALRGPNWTQLILGSEGTLGI
                     ITSARLRVSPAPQLRVFRGFEVDDVQTGIEAIRRVLQKGLRPAVVRLYDELDTLINSF
                     GHEHSDESQPSRAPAPPISTGALPAYPTPERPKDDGLVDRLLGLAKGKGLRQNAMKAA
                     LGKSELVNRVFGTVAEKIARRGCRMIIGLEGARIRTEIEAALTFAELEAAGARDKGED
                     PGWAWLKSRYHVSYRMSPVFRDGAFVDTMEVASSWERLGDLYHSVRTAIGHHAVVMAH
                     FSHAYADGCSIYFTFAGHAPEAAAAERIYDAIWRDGLEATTRVGGTISHHHGVGLLKA
                     PFMAGEHREAMAIFEAAKASLDPDGIFNPGKMGLRSNR"
     gene            10233..>10489
                     /locus_tag="VFV99_30440"
     CDS             10233..>10489
                     /locus_tag="VFV99_30440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063751.1"
                     /translation="MSLYNELVTAVGTEYVEPLPELAQPPCFRVVPGSAAEVAEVIRR
                     AGASGAAIHPVGGGGRPSRVDKDPKGERSRVFIATHRLDQVL"
BASE COUNT         1728 a         3511 c         3456 g         1794 t
ORIGIN      
        1 gcctcgaccc acggctcctt cttgctaccg aacagctgga ccatgaagcg gcccatcgcc
       61 gtgttcgagc cggtgagctt cgcgcgcacc gcgaacgcgt cgagcgcctc gatcatctcc
      121 tggccgctct ggaaccgcgc gctgggatcg ttcgccatcg cggtgagcac gatcgcctcg
      181 agctcgcgcg ggtagcccgg caccgcgacc gacggcaaca tgacatcgcc ggcgacgatg
      241 cgtttcatcg tctcgaagtc gtcgttgcct ttgaacgcgc ggcgcaacgt cgtcagctcg
      301 tacagacaaa tgccgagcgc gaagatgtcg ctgcgtcgat cgatcggctt gcccttgcac
      361 tgctcgggcg acatgtagcc gtacttgcct ttgatcgtac cgccgaccgt cttcgtcgcg
      421 cgctcctcgg ctttcgcgat gccgaagtcg agcaccttca cgctgccgtc gtagccgacc
      481 atgatgttcg ccggcgagac gtcgcggtgc acgatgttga gtggcgcgcc gttcttgccc
      541 ttgcgctcgt gcgcgtggtg cagaccagcg gccgcaccgc tgatgatcat caccgcgagc
      601 tcgagtggaa tcgtccaacc gcgccgcagc gtggtctcga gaatcgcgcg tgcgttctcg
      661 ccgtggacgt actccatcgc catgaagtac gcgccgtccg cctgatcgac ctcgtacact
      721 tgcgcgatgt tctggtggtt gagcgtcgcc gcgagcttcg cctcgtcgag gaacatcgtg
      781 acgaagcgct gatcggtggc gcgctcgcgc aagatcgtct tcagcacgac gtggcgctcg
      841 aacgcaccga gaccggtctg ccgcgcgaga tagatctgcg ccataccgcc tgtggcgagg
      901 tgacttagca cctcgtagcg gccaactcgc gtagggatgc ctggagatgg ctccggcggc
      961 gccacacgtg aaggatcgaa cgcttggctc aacgtgtcaa gcgagcccgg gggtaacgac
     1021 ctccgtcggc gtcgccctaa aggaccatct tcaagaacga gatgaccttg tcgagctcgt
     1081 ccgcggtcag cgccgctgcg atccgggcaa acgactgctg ctggtcgatc gttacagaat
     1141 cagcgaaatc cttacccacg aacccctcga gctgcccccg gcggtcgggt gtgagctggt
     1201 cgttgacggt cttcaccgcg gttgccgccg cggtctcgtc cgcgaacttg aggcgcgcat
     1261 cgatggtgag tttctgggcc atcgcgaacg agacggcagc ccactgcggc tcggctccga
     1321 gcgacttatt gaactgttct ttcatcgagg catcgagggt cgccccgccg aacaccgccg
     1381 tgttcggggt agtcgcggcc atgatcgtcc ggacagcagc cggccctttg gccttcttgt
     1441 ccttcgcacg atcgatgacg ccgctgatct gacccgcgct cgcgaacacg agcttcttgt
     1501 cgaccaccgt gaattcgcca tccgcggtga cccagaacgt cacgcccgcg tgcgtgtctg
     1561 ttttcgtcgc ttgcttcttt gcctcggcga cgagcttgtc gatgcggcct tcgagcacga
     1621 tcaccgcgtc tttgttcggc gtgctggcgg cgacgatcgt atcgacctgc ttctcgatcg
     1681 gctgcgccga ggcgagcgtg tcgagcgtgg tgttctgctc gcgcgcgatc gtgaacagct
     1741 tcttgaagat cggtgaattg cgcgcccgcg ccacatccgc aacgacgacg acgttcgtgt
     1801 cgtcgggcag atacttgagc gatgcagtgc cgcgaccggc gtcggcacgc gaagcgacga
     1861 gcgcgatcaa gagcgtgacg agcgcgtaac gcatctctgt agtgtggatc agacggctgc
     1921 cccagacctg gcattcatcg gtcgaactag cggatactcc gtaggcggtg gggacggcaa
     1981 actggccgat cgcgttatct ggcgccattc cagcgctggt tgcgatgtgg ttcgtcgatc
     2041 gcatggatcg caagcgtccc gagcccgtgc gcacacgccg gctcgtcgcg ctcgcgggca
     2101 tgctgtcggt gatcccggca ctgattctcg agatcgcggt cggcaacacg ctcggcgaac
     2161 gggtcgggcc cgcgatgacg tatcagggcg cgacgttcaa agcgttcatc gtcgcggccg
     2221 cgatcgagga agcgtgcaag atcgccgtcg tctactgggt cgtgtggcgg cggcccgagt
     2281 tcgacgagcg gatggacggc atcgtctacg cgagtcgcgc cggcctcggc ttcgcgctcg
     2341 tcgagaacat cttctacctg ttgcagcagg gcacgctcgg cggccagctg cagacgtgga
     2401 tcctgcgcgc ggtactcgcg gtgcccggcc acgcgatgtg gaccggcatg atcggcgcga
     2461 tggctgcgcg ccgccggttc gacggcaagg gtctcggttt gatcggcggc tacctgctcg
     2521 cggtctcgtt ccacggcgcc tacgacctgt gcgcgttcct ccagcagccg ctgcacctcg
     2581 aaggctatga cgaatacgcg cccggactga tgcctgcgct catcggcctc acgctactcg
     2641 cgttcttcgt gctgcgctcg caagcgcgta tggcgctgaa gctcgacgat gcggacgcag
     2701 cgctcaacgc cgcgcacgcc gcaagaacaa cacaatagcg gcgacgccgg cgacgatcgc
     2761 gccgacgtag aacaagaagc cggccgcgac gctcgcgcca ccgacgccgg gatagccgac
     2821 atagaaatat gcgccggtag cgatcgcggt gacgatcgcc gcgagacagc cacgcgcgac
     2881 gatcttcggc tcgcgcttcg ctgcgatcgc gccggcgaac atgatcagca cgaacatcgc
     2941 gatgtaaccg gccgcgcgtg tcgcgacagc actgcgcatc cacagctcgc tgccaccggt
     3001 ccacgcaagt cctgcctcgc ggcattcgcc gccgaagcag tggcgcgaac cgaacgggcc
     3061 aatcgcaact tcagcgatcc cccaccatgc ggtctgcact gcaatcgcga acgaggtagc
     3121 ggctgcgagt gcgagaccta ccgtgaacca ccgagatcta ctgtccatgg aattcgcgcg
     3181 catattggat agctattgtt tgaggttcaa ccaatttcta gcacctgctc acagacgccg
     3241 atttagcgtg atgcctacat tgaccgccga tatgtaggca cacgaatgat cttcgtcggc
     3301 cgagatccat gcgcagtttg ctcgtactga ccatcctcgc tgctctatct ggcaccagca
     3361 ccgctgctcc gcaatcgctg gtccttgtgg accgcgtgct cgcagaggcc gaggaggaga
     3421 ttcctgatct cacgatgctg acgaccgagc cggtcgagcg caccgagagc tcgggatacg
     3481 gctggcgtga ggacccgatc aacaagcgcg cgaagttcca tcacggatcc gattaccgcg
     3541 gcaagcgcgg cacgcccgtt ctcgctgcgg gcaagggcac ggtgatcttc gcgggtcgcc
     3601 gcggtggcta cggccgcgtg gtgttcatcg atcacggcgg tggcgtcatc acgcgctacg
     3661 ctcacctcga ccgcatcgac gtcaaagttg gcgatgcgct gctcgcaggc atgcagctcg
     3721 gcaagctcgg ctcgagtggc cgcacgactg gcgcgcatct gcacttcgaa gtgcgactgg
     3781 atggccgcga cgtcgatccg aacaccgcga tgatcgtcgc cgacatcatg cgcgagaacc
     3841 cggcagccgg ccggctcgcc gcattcgcgc tggcgcccga gctgcaagag cattcgcgag
     3901 acgccgagag cccggacatt cgcgtaccct cgcgccccga acgtgctggc cgcacgaagc
     3961 gccagcaggt cttgtggtag caggcacttt ggcgagctag gctgcgcggg tggttgcccg
     4021 ctcgccggac ctgctcgcac gacgatacgt cgcgtggctg cgccggcgcg cttctgcggt
     4081 catcgccgcg cacgtggtcc tcctcgcgct atcggtctat ctgatcgcgt tccggctccc
     4141 gctgttcgcg gacttctcgt acttgttgcc gcaagacgtt ccggccgtga aggacctgcg
     4201 caagctcgaa gcgcgggtca agtcgaccga cagcgtgctc gtcctcgtca cggccccgac
     4261 gcctgccgcg cgcgaagcag cgacccagga agtgatcgac ggcctcgccg cgctgcactc
     4321 gccgctgatc gcccgcatca gcggtgacga gatcgcgatc cgcgagttcc tgcaggcgag
     4381 gcggcatctg ttcgtgccgc tcgacgagct cgaacaggcg cgcgacacac tgcagaagaa
     4441 gctcgacgac gagaagctgc acgcgaaccc gctgttcatc gagctcgacg acaaagatcc
     4501 gaatcaggcg gcggccgatc aaaagcagct cgatgatctg cgcaagaagc gccaggacgc
     4561 ggaagcgaag ctcgcgcgac cgacgaacgt ctgcgccgat ggactgactg cacgggtcga
     4621 tgtcagcacg tcgttccgta cgaccgacgc gaagtccggt gcagagctac tcgcccagct
     4681 caccggtgtg cgcgcgcagg tgatcgcgaa gcatccaggc gtgaagatcg gcttcaccgg
     4741 ctcgccgatc accgcgctgt cggagcatca ctcgatcacg aacggcatcg tcctatcgag
     4801 cctcgtcacc gcggggctcg tcgcgctcgt gctcgcgctg tttttccgca gcgcgacgtt
     4861 gctcgtgttg ctcgtcagca cgatcgcgat cgcgactgct gccgcgttcg gtagcgcagc
     4921 gttcacggtc ggccacttga acgcagcgac cgcattcctc ggcgcgatca tcgcgggcaa
     4981 cggcatcaac tacggcattc tgctcatcgc gcggtatctc gaagagcgcc ggcgcgccga
     5041 cgtcgacgag gcactcgcgc acgcgatcgt cggaacgctg cgaccgacag ccgtcgcgtc
     5101 cctcggcgcg gcgatcgcgt acggctcgct cgcggcgacg agcttcaagg gctttgccga
     5161 cttcgcgatc atcggcgcga ccggcatgat gctgtgctgg gttgcgtcgt acgtactgtt
     5221 gccggcgctg atgctcgagt tcggccgcga cacgaagatc ttccaaggca acccgcgcat
     5281 cggctccgca ctcgtgcggc tgctcggctt caagcgctcg ggcgtcgtga tcgcggtcgc
     5341 gtcggtggtg ttcgcgctcg cgaccgtcgt cgtcgtgcgc tacgtggcag cggacccgtt
     5401 cgaatacgac attcgcaacc tgcgctccga ggcgagcgac gcgatcgaag cgcgccagtg
     5461 gatgaaaatc tcggacgagg cgttcggccg cggctactcc gggcgcacgt acatcgcagc
     5521 ggaccggctc gatcaagtgc cgatgatcat cgacgcgctg cggacgctcg acaccggcaa
     5581 gccggaggca cagcggacga tcggcgcgat cgaatcggtg ctgacgtttg ttccacaaga
     5641 tcaagacaag cgcatcgcgg tgctcgacga gattcgctcc ctgatcgatg gcgccttcga
     5701 cgcgatggac gataaggagc gcgccgagct aggcgagttg cgtccacccg agggcctgca
     5761 cccgttcacc gcgaacgagc taccgccgac gctgcgcgac aagctcaccg agaaggacgg
     5821 tcgcatcggc tacctgatcg cgatccgccc cgccccgcta ctcgacgaat ggaacggcaa
     5881 ggacctgatc cggttcgcgt cggcggtgcg cgagctgcat ctgcgcgaca acgagaccgt
     5941 cacgacgtcg ggctcgagcg tgatcttcgc cgacatcgtc gagtcgatcg agcgcgacgg
     6001 tccgctcgtg accggcgtcg cgttgctcgg cctcatcgtc atggtcgtgt tgcttgtcgg
     6061 ccggaaccgc cgtgcggtcg cggtgctcgt cggtacggtc ggcgggtcgc tactcatgat
     6121 cgccgtgtgc gcgctgctcg atctcaaagt gaacttcctc gatttcgtcg cgctgccgat
     6181 cacgctcggc ctcggcatcg actacgcgat caacgtcgcg caccgccacg acaccgaaga
     6241 gctccccgat ccgatcacga cgctcaagac gagcggcagc gcggtgttcg tctgctcgct
     6301 gacgacgatg atcggctacg gctcgctgct cgtgagcgca aacctcgcga ttcgcggctt
     6361 tggcaccgct tcgttgatcg gcgaggtgac gaccgtcatg accgcgcttg tcgtcgttcc
     6421 ggctcttctc gcggtcggca ggaagaccag cgtacaatcc attcggtcgc gctgaccaac
     6481 gcgtcgccgg cgccaatctc gagctaaccg acgcgcttgc cgtctgcgct ccagcgatag
     6541 aagccgacgc cggtcttctt gccgagctgg ccggctgcga ccttgttccg caacagctga
     6601 ggcggatcga agctggggcc gatcgcgctc gcgaggtact cggcaatgcc gaggcggacg
     6661 tcgagaccga ccagatcggt cagctcgagc ggtcccatcg gatggccgta gccgagcttc
     6721 atcgcggtat cgatgtccgc cgccgacgcg acgccctgct ccaccatgcg gatcgcctcg
     6781 agcccgatca cgatcccgag tcggctcgac gcaaagcccg ggctgtcctt cacggtgatc
     6841 ggcgttttgc ccattttctc cgcgagcgcg accgcgagcg cgaccgccgc gtcgctcgct
     6901 ttgccgtgcc gcacgatctc gacgagcttc atcacatgga ccgggttgaa gaaatgcatg
     6961 ccgacgagcc ggctgccgtc gcgcactgcc tcggcgatat cgctgatcgg cagcgacgac
     7021 gtgttcgtgg ccaacagcgc atgcgcaggc gcagcttcgt cgatcacggc aaacgtctcg
     7081 cgcttgagct cgatgcgttc cggaattgct tcgatcacgc agtcgacgcc ttcgaccgtt
     7141 gcggcgagtg catcgcgggt cgagatgcga gccatcgtcg cgtcgcggtc cgccgcgctc
     7201 accttgccga gctcgacgcc cttcgcgagg ttcttcgcga tgcgatcgac gcccgcgcga
     7261 gccgcgccat ccagcgcgtc gtacaggcgt gtttgggcgc ccatttgcgc agccacttgc
     7321 gcgatgccgt gccccatggt gccggccccg atcactgcaa tcgttcgtag ttgttcgagc
     7381 gtgggaagca tcgcgagaga acatacgtca aagcggcccg aggttcgtta gactagggcc
     7441 gtgctcgcac ggtggacatg cctggcggcc gcgctgaccg ggtgcagttt cagccacggc
     7501 gtggccgtcg atccgacgat cgaggacggc catatcgacg acatcgatgc gccaccggcg
     7561 gatgcccgcc ccgatacgcc acctggcgcg acctgctatg gcaccggctt gttcgtcgat
     7621 tgctacgagc ccggcacgca gccgacgaac agcgacacgc tgtcgagcgg catgatcgac
     7681 acgagcaacg cgtcgaactg cacgcgcacg gtgccgcagt caaacggccc ggagatctgc
     7741 ctgcgcacgg ccgttgcgat cacggtcaat ggttacgtcc gattcaaagg ctcgcgaccg
     7801 ctcgtgctgc tcgcgaccag ttcgatcacg atcaccacgg caggtacgct cgacgtgtcg
     7861 tcgttccgca acgacgtcgg cgccggcggc gataccgggc cgtgcgcgaa tgtcgggaat
     7921 ggcagcgcgg atctgagccc gagcacgaac gcgggcgcag gcggcgcagg cgccggtggc
     7981 ttcgggacac cgggtggtac cggcggaagc agcaatgccg gcggcggtgg cggcggtggc
     8041 acgaccgcgc tcacgatgat ccgcggcggc tgtgccggtg gcaaaggcgg caccagcaac
     8101 ttcggcgcag gcggcgatgg tggccacggc ggcggcggcg tctatctgat cgcgggtagc
     8161 tcgatcacgg ttgtcggaac gatcaaagcg aacggcagcg gcggcaacgg cggcatttac
     8221 aagtctggcg gcggcggtgc gggctcgggc ggcctcgtcg cgttcgatgc accgacgctc
     8281 acgatcaccg gcaccgtcta cgcaaacggc ggaggcggcg gcgaaggcgg cggcgacaac
     8341 aggaccggcg agaacggctc tgacccgtcc gcgtacaacc agcgcgcggg cggcggcaac
     8401 tcacggctca gcggcggcga tggtggcagc ggctcggtgt tcgcgaactc gggagccggc
     8461 ggcggtaccg caaataacgg agcaggcggt ggtggcggcg gcgcaggccg catcaaggtc
     8521 taccaagcga gtgtgccgac cggaaaactc tcgccgccgg ctaactgaat cgcacacgtt
     8581 tggtaccttg cgaacgtgca accagctgtc gacgtcgatc gactccagcg cgagctcgaa
     8641 tcgatcttcg gcccgcgccg cgtgtcgctg cgtgcggtcg acctcgacac ctatgcgcgc
     8701 gacatgtggc cgcggttgct catcgcgtat cgcgacggca ttccgccgcc gcatcgtccg
     8761 cacgcggtcg tgtggcccga atcggtgcgc gaggtcgtcg cggccgtgcg actcgcgcgc
     8821 gagctgcggc tgccgatcgt accgtacggc ggcggctcgg gcgtgtgcgg cggtgcggtg
     8881 ccgctctacg gcggcatcac gatcgatacg aagcgcatgc aggccgtgcg cgcggtgcag
     8941 accgacgagc tggtgtgcga cgtcgaggcg ggcctctctg gcgaacgctt cgagcgcgag
     9001 ctcgagcgcc gcggttacac gttcggtcac tttccgtcgt cgatctattg ctcgaccgtc
     9061 ggtggttggc tcgcgtgccg cgcggccggc cagctctcga cgaagtatgg caaggtcgaa
     9121 gaccgcgtcg cgggtctgac cgtcgtgacc ggccgtggcg atgtgatcga gaccgatggt
     9181 ccgaaccgcg cgctgcgcgg accgaactgg acgcagctga tcctcggcag cgaaggcacg
     9241 ctcggcatca tcacgtcggc gcggctacgc gtgtcgcccg cgccgcagct gcgcgtgttt
     9301 cgcggcttcg aggtcgacga cgtccagacc ggcatcgagg cgatccgccg cgtgctgcag
     9361 aaaggcctgc gcccggcggt ggtgcggctc tacgacgagc tcgacacgct gatcaattca
     9421 ttcgggcacg agcacagcga cgagtcgcag ccgagccgcg caccggcgcc gccgatctcg
     9481 accggcgcac tgcccgcgta tcccacgccc gagcgcccga aggacgacgg cctcgtcgat
     9541 cgcttgctgg ggctcgcgaa gggcaaaggc ctgcgtcaga acgcgatgaa ggcggcgctc
     9601 ggcaagagcg agctcgtcaa tcgcgtgttc ggcacggtcg ccgagaagat cgcgcgccgc
     9661 ggctgccgca tgatcatcgg cctcgaaggc gcacgcattc gcaccgagat cgaagccgcg
     9721 ctgacgttcg ccgagctcga agcggctggc gcgcgcgaca agggcgagga cccgggctgg
     9781 gcgtggctca agagccgcta tcacgtgagc tatcggatgt cgccggtgtt ccgcgacggc
     9841 gcgttcgtcg acacgatgga agtcgcgtcg agctgggagc gcctcggcga tctctatcat
     9901 tcggtgcgca cggcgatcgg tcatcacgcg gtcgtgatgg cccacttctc tcacgcctac
     9961 gccgacggct gttcgatcta cttcacgttc gccggccacg cgcccgaggc ggcggctgct
    10021 gaacgcatct acgacgcgat ctggcgcgac ggcctcgaag cgacgacgcg cgtcggcggc
    10081 acgatcagtc accaccacgg cgtcggcctg ttgaaggcgc cgttcatggc cggcgagcat
    10141 cgcgaggcga tggcgatctt cgaagccgcg aaagcgagcc tcgatccgga cggcatcttc
    10201 aacccgggca agatgggcct gcggagcaac cgatgagcct ctacaacgag ctcgtcaccg
    10261 ccgtcggcac ggagtacgtc gagccgttgc ccgagctcgc gcagccgccg tgctttcgcg
    10321 tcgtgcccgg atcggcggcc gaagtcgccg aggtgatccg ccgtgcgggt gcgagcggcg
    10381 cagccataca tcccgtcggt ggcggcggcc ggccgtcgcg cgtcgacaaa gatcccaaag
    10441 gcgagcgctc gcgcgtattc atcgcgacgc accgcctcga tcaggtgct
//
LOCUS       DASUFX010000752        14180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1789141, whole genome shotgun sequence.
ACCESSION   DASUFX010000752 DASUFX010000000
VERSION     DASUFX010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789141"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFV99_30445"
     CDS             <1..529
                     /locus_tag="VFV99_30445"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063752.1"
                     /translation="EPTPDELAQHAAALSVGYNEPRNAELMGHTSAISPAEVIENYTE
                     TIAGGMRAFLLFSGGELAGDADLRGLRGGSGEFAFMIAMPSAQGKGLGTRFATMIHAF
                     GFRALGLSRIYASVVPHNTASRRVFEKLGYVVDNSAAARAYADEPDDVTLALDRATFE
                     RIAGSALADIRAGVR"
     gene            533..889
                     /locus_tag="VFV99_30450"
     CDS             533..889
                     /locus_tag="VFV99_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX5063753.1"
                     /translation="MATASQVLTGLVAFLHVYFLVLEMFLWATPFGAKTFKRPLDEMQ
                     QTKVLAANQGLYNGFLAAGLVWSFFATDGYEIRVFFLGCVVVAGLYGAVTASRSILFV
                     QALPAIAALVVTLLAH"
     gene            968..1264
                     /locus_tag="VFV99_30455"
     CDS             968..1264
                     /locus_tag="VFV99_30455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063754.1"
                     /translation="MARKAAKNVPGTERYRDERRTGGGPRMHEREWHEHDQPHEAERI
                     ANSEGFDAIPEVESGGDRAGMRRKQAQTPTSQGGERRKRSVPPSKGPKRGAKRI"
     gene            complement(1291..2055)
                     /locus_tag="VFV99_30460"
     CDS             complement(1291..2055)
                     /locus_tag="VFV99_30460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5063755.1"
                     /translation="MKVATWNVNGIRARSSQVAHWIDGEEPDVICLQEIKATVAQIPE
                     PLDRIAGYHAHWHGSGGYSGVALMLRTAKFPDPPEFVHPPFDLETRIVVAEVGSFVFA
                     SVYVPNGGKDFEAKMVFLRELAAWTGELEKAGKHLVLCGDFNVARSPIDVHPSLRKDM
                     VGQLKIERDLIDGLFSHGLVDVGRHVDPDNDRLFTWWAPWRNMRQRNIGWRLDYVAAH
                     HALVDGAVTCKSYREVGSSDHAPVIAHLRDEPLPSS"
     gene            complement(2074..3141)
                     /locus_tag="VFV99_30465"
     CDS             complement(2074..3141)
                     /locus_tag="VFV99_30465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063756.1"
                     /translation="MKHVYPLVLLLAGCTSGLTTDPQPVVSVLKQDGDCFALMTVDSP
                     ISPILGISGTCNYTATPKLFAGADFIEVVVDYGDVAFSGTTSAPRPDVVVTVDGVVSD
                     VPIEISDEYRIGGHAYFIATFYAPLESSSNVRISAGVNAGFQTVSPVVFSTIPPPVGF
                     TLLDCAQGSQCDLPGATGSAHVFISVPGQVSQLVSIHAVLGGITQPDPVPPARTQVVL
                     GHSEVTTAVPIPNAPDQTTWVLAAQLGDGIPTEVSATIRAPTLITKLSCGTACNLAPG
                     DVVGLDITAPTGIRPLEALVDTRINGVPQLVGAPVTLVQHADGTAVGALALTAPTTTG
                     SWQIDVTIAGYPAPAIVTSVQ"
     gene            complement(3138..5144)
                     /locus_tag="VFV99_30470"
     CDS             complement(3138..5144)
                     /locus_tag="VFV99_30470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063757.1"
                     /translation="MAVALPIALALPRESAAGEIPIITLGDSGPDQEIPTDRSFYIAG
                     RAGSSVVSAQAVVVRRGSSSLFGDDGPDCHDVIADLRIETTTATAADDEEGEDEADDS
                     SAMPRYDSGPHRAFEIFPNATGDSRRAPVLVSSAWQRGHDDERQFNMLVPYDRSFFSA
                     GYGYCLFVVTSEHSQELDHATIVDLFDDVASKIVTCGDKASCDEDALGDYEARVTKAL
                     FATDSARVPGRVSELADRLREAARVELGSATGIVEAIDRMGDRWHDKTTVMTPTVTVV
                     WAEVANDPFAHAVAALLARSGALLPQVRSQGASSSVALFTADGKLQVKAIQLLDDGRS
                     IRVAASKAPSGAQARVLTATTDTLAIADGLTLYDLIQLGNRRIRVDKEWVSLKDLGER
                     MANLGTASWSADDSAYLVTAGAHMKRLANFVDLTTTGATCTTKTFAVSEAEQSIDAIR
                     QHLGEWLVCQKVDAAAIETMREQLEELTHEEDSWRATKDKLVARSQRIVTLTTTEPTG
                     MRVSFASRTWFFSYVTPMIGWASVVQPDDSFGLFYFGAQIHLAPNPVDDPQWRDGITA
                     KDLRRAIALEVGIAPYQSSFGPEMRYGGSGSLPPLFFGAAVHVLPYTSVTFGGSVLDK
                     RSSTLLEEQPHTIFAPYIGLSLQLNLPDLVRDASHPTSDTTATR"
     gene            5449..6042
                     /locus_tag="VFV99_30475"
     CDS             5449..6042
                     /locus_tag="VFV99_30475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5063758.1"
                     /translation="MARRALRDKPSADRILTAAETLFANKGYGEVSLRQLIAAAGVST
                     TAFYARFDSKASVLDTLTERLFAELQLEAAMELRNVRDFATGIERGVDILCARFGPRK
                     ALVRLVVAESGSLAPMLSTRRKSYAMLAGFLAHYFKLLADRGRITVADPNALAWALVG
                     ALEIQIVRWAVWDELELDQLRDELLAVTRAILPKEKS"
     gene            6039..8249
                     /locus_tag="VFV99_30480"
     CDS             6039..8249
                     /locus_tag="VFV99_30480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063759.1"
                     /translation="MTRIDRLIATAVLAAAVPAAVVVVGCSSDPGTTVVMTTADEAPA
                     YGTTPFPTDALREGNHLATLRGLNALLDRHADIIAAHFAALDGFGVRPLVEIFVTGAL
                     DEASLPAHTAALADAAVVLDVDPDSPERGRVIAMDWHYDAERLVLQGAPTSGEVLREG
                     TRYAAFVTSAIKPLRRASAFDDLPSHGRWQTTAEALHTLDAMAGIDDDIVGITVFTTQ
                     HATAPLLAARTIVEQLPPSDLVFDDPAIIFNSTAALDKVIGVATRATDGPRVGLERWG
                     NDNPTGIAHDHVGVIGTGKMTIARFRSDDSKTDLPDDETFSDPPHVVAMDTIPVTFIL
                     PKAAMPPGGYPIVIYGHGLGASRDQLLSFAEPLTSRGYAIVGIDMAGHGSRFDPTDQL
                     ANMANQLTAFSGDAGTRDGFGDTTGLVTQFDFFEGFLGVSAVRDSIRQSALDLGRVAQ
                     MIRRTDLDLSALAGPGSANPKLDTRHIAYLGESFGTVVGSVFSAIEPNIDLYVLDVPG
                     GGILDHILTSSPEIAATAVPLIDSILGPRQRVDRFNPLIGLMQSVIDGADPLSYAPHV
                     LRDRFAINGTVLGPRSVVCLEVLGDQVLANVGTDSLARELGLDVLVPNLAPPDGLVPI
                     ASPAAGNRDGMTAVLVQYSPATHGANWSAEHGVLRYLPGFPFDDENQFPKLPAPVTIR
                     NPIYETHAQVAEILETYFAGESPRVRSTLAPVADFDDDGTPDATDPAPYDPAQR"
     gene            complement(8260..9003)
                     /locus_tag="VFV99_30485"
     CDS             complement(8260..9003)
                     /locus_tag="VFV99_30485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063760.1"
                     /translation="MGRGTREQANELRAVAPLVVTAPTLQLVGPCTLVTNGTQTIAFS
                     SAELLRKAGEPLAIALTLDCSKTLPVSSWSMGRVPHMGVIELGQPFPKDKKLDVEPLP
                     IGSLCATVDTRGAPSALVTIERATQGVSRRVISVHVDNVDGGGMSDDVLVRLASPDDA
                     ADSEADIDGAALFSWMPPDPVLGRKSEVLAVALASPYRTKTFKPRELAAIAELFGLDD
                     LGRALPWDGTRAEGSNELDQVAGEIRKGS"
     gene            9147..9665
                     /locus_tag="VFV99_30490"
     CDS             9147..9665
                     /locus_tag="VFV99_30490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5063761.1"
                     /translation="MVGVDLTEYSHIVIDHALDQAAHHRAPELHFVHVKESRRHDVNT
                     LGERLSAAVYPSLQVFNEYGKDWRARLHIRKGRPDEQITMLAADILADMIVIGDFGLH
                     RKGMPKRILTHAICPTLVVGMPKLLDTRQCGECAALREDSEGTRWFCDDHRVKQNAVS
                     PMTTWSNGSLMQ"
     gene            9699..11003
                     /locus_tag="VFV99_30495"
     CDS             9699..11003
                     /locus_tag="VFV99_30495"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5063762.1"
                     /translation="MPATAEEPHAPPPPRSLRRALVTTFRGLLRLYFRRIERVGEAPG
                     AETRGRMFVSNHHNALIDPVLVLTDAECEISPIAKSTLWSIPGLKYLLDSAGAVPLYR
                     RKDDPNKATSANDAVFAKIASHLGGGGNILIFPEGTSHSEPHLAPLRTGAARMLMAAG
                     AYGSPLTFQAVALEFDEKDRFRSRCLVLWGPVRKLADVGGSGEDRVRAITAQMDTDLR
                     ELLVEGDTHDERMLIARVAEMLANDAGDGSLSGWSTIGRQVELAGRSLREADPALVAH
                     VREKVDAYYAELARFGRVDAQLSATRAAVKKRPARWLRRAALAPLAVPGFALYAVPYL
                     IPRLVARTSDPDAVSTVKLATALVVYPLWAAGLVGLSLVLVPPPLSLVAAGLVLVSPF
                     AALRWLDAYWERTPEPTADELAQLARLRIAARTAIDEARSRL"
     gene            11045..11878
                     /locus_tag="VFV99_30500"
     CDS             11045..11878
                     /locus_tag="VFV99_30500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063763.1"
                     /translation="MRALLVLLALAAPASAAASSEDDWLTGLTDRVVDDLTAGKSLNV
                     LVHVPLCDNTIIACGNAKLGDGDTPSTNLYWSTTPGFGQWFQRKGSGWTRVSVETKDT
                     GDKDVLEKHIYRRTIATPAAWRKRGAPKQFTLEVEIRAWRGSAIDRALAAYANDVSGK
                     GSSAQLVAFVGHNRLMDLDTFAWPEPGKDVKGAIAIACDTAPYMKKQVSAATRVPLLM
                     TADLLFANAAPLEAAVLAFARGGGYAQIRKDAATAYAGVRKRPVEKVIGAFTNPGDRR
                     W"
     gene            complement(11879..12427)
                     /locus_tag="VFV99_30505"
     CDS             complement(11879..12427)
                     /locus_tag="VFV99_30505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063764.1"
                     /translation="MSYRQLPTTSRRIGFEIETPEQVEVIDHPGAGLVKCSERRDGKT
                     VGELEVAVFHAALVIDRDGILEEKAASAIESAREPGARVSPAMPIALPGASGYRAELE
                     VVRPAGVAKAPLPYVYVFAMAPHDLALDGGLIVTVRCASPEWPAADEILRSLRILSRG
                     GKPAANDADVRSGLPLIGSRDE"
     gene            complement(12507..>14180)
                     /locus_tag="VFV99_30510"
     CDS             complement(12507..>14180)
                     /locus_tag="VFV99_30510"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5063765.1"
                     /translation="MSVKLALAVAPVDKKNEEVVAKDVAKERPKADNKPAEKTADVSR
                     HMGPPAPPPMTVAPSPSSPSVGMAGGRMSRRQPLQVEQDPSMNTEAYSRIDDNPFLRV
                     DQKPLSTFSSDVDTASYANLRRFIRDGQLPPKDAVRIEEMINYFHYDLPAPQKGQPFS
                     ITTEVGPSPFNSKFQLVRIGLKAPEINDDKVPARNLVFLLDVSGSMMVANKLPLVKRA
                     MNLLVENMRDNDRIAIVVYAGASGLALPSTTGKEKDAIRQAIQALEAGGSTNGAAGIK
                     LAYEIANKNFIKGGINRVILATDGDFNVGTTSEGDLTRLIEEKREHGVFLSVLGFGMG
                     NLKDSTMEKLADRGNGNYAYIDTIEEARKVLVKEAGATLVTVAKDVKFQVEFNPQTVV
                     GYRLIGYENRLMRDEDFNDDKKDAGDIGAGHSVTALYEVVPAGVEVPGGKVDNLKYQG
                     KAQPTGGNADELMTVKIRYKAPDGNQSKLLSQAVKQSKVEMAKTSIDFRWAAAVAGFG
                     MMLREAKDRGNISWATVTQLAEGAKGQDAEGYRRQMIEMLKRAARLPKS"
BASE COUNT         2345 a         4856 c         4554 g         2425 t
ORIGIN      
        1 cgagcccacg cccgacgagc tcgcgcagca cgcggccgcg ctgtctgttg gctacaacga
       61 gccgcgcaat gccgagctga tgggacacac gtccgcgatc tcgccggcgg aagtgatcga
      121 gaactacacc gagacgatcg caggtggcat gcgcgcgttt ctgttgttct cgggtggcga
      181 gctcgccggc gacgccgact tacgcggcct gcgcggcggc agtggcgagt tcgcattcat
      241 gatcgcgatg ccgagcgcgc agggcaaagg tctcggcact cgcttcgcga cgatgatcca
      301 cgcgttcgga tttcgcgcgc tcggcctctc ccgcatctac gcgtccgtcg tgccgcacaa
      361 caccgcgtcg cggcgcgtgt tcgaaaagct cggctacgtc gtcgacaaca gcgcggccgc
      421 gcgcgcctac gccgacgagc ccgacgacgt gacgctggcc ctcgatcgcg ccacgttcga
      481 acggatcgcc ggatctgccc tcgccgatat ccgcgccgga gtacgctagg tcatggccac
      541 tgcgtcgcag gtgctgaccg ggctcgtcgc gttcctccac gtctacttcc tcgtgctcga
      601 gatgttcttg tgggcgacac cgttcggcgc caagacattc aagcgtccgc tcgacgagat
      661 gcagcagacg aaggtgctag cggcgaacca aggcctctac aacggctttc tcgccgccgg
      721 actcgtgtgg tcattcttcg cgaccgacgg ctacgagatc cgcgtgttct tcctcggctg
      781 cgtcgtcgtc gccgggctct acggcgcggt gaccgcatcg cgcagcatcc tgttcgtgca
      841 ggccttgccc gcgatcgcgg cgctcgtcgt cacgcttctc gcgcactgag cagatctccg
      901 gactccggac cgtacattct cgcaagggca cgctgcttgc ttctggtttc tgagcaggag
      961 aaccagcatg gctcgcaaag cagcgaagaa cgtgcccggg accgagcggt atcgcgacga
     1021 acgccgcacc ggcggcggtc cgcgcatgca cgagcgcgag tggcacgagc acgaccagcc
     1081 gcacgaggcc gaacgcattg ccaacagcga aggcttcgac gcgattccgg aggtcgagtc
     1141 gggcggcgat cgcgcgggca tgcggcgcaa gcaagcgcag actccaacct cgcaaggtgg
     1201 cgagcgccgc aagcgttcgg tgccaccgag caagggaccc aaacgcggag ccaaacggat
     1261 ctaggacgcg gatgctcgtc ggcggcgcgg ctagctcgac ggtaacggtt cgtcacgcaa
     1321 atgcgcgatg acgggcgcgt gatcgctcga gccgacctcg cgataggatt tacaggtcac
     1381 cgcgccgtcg acgagtgcgt gatgcgcagc gacgtaatcg agtcgccaac cgatgttgcg
     1441 ctggcgcatg ttgcgccatg gcgcccacca cgtgaacagc cgatcattgt ccggatcgac
     1501 atggcggccg acgtcgacga ggccgtgcga gaacagcccg tcgatcagat cgcgctcgat
     1561 cttcagctgg ccgaccatgt ccttgcgtag cgacgggtga acgtcgattg gcgagcgcgc
     1621 gacgttgaaa tcaccgcaca gcacgaggtg tttgccggcc ttctcgagct cgccggtcca
     1681 cgccgccagc tcgcgcaaaa acaccatctt cgcctcgaag tctttgccgc cgttcggcac
     1741 gtacaccgac gcgaacacga agctgccgac ctcagcgacg acgatgcgcg tctcgagatc
     1801 gaacggcgga tgcacgaact cgggcggatc cggaaacttc gcggtgcgca gcatcagcgc
     1861 gacgccggaa tagccaccgc taccgtgcca gtgtgcgtgg tagcccgcga tccggtcgag
     1921 cggctcgggg atctgcgcga cggtcgcctt gatctcctgc aggcagatca cgtccggctc
     1981 ctcgccgtcg atccagtgcg cgacctgcga ggatcgcgca cggattccgt tgacgttcca
     2041 ggtcgcgacc ttcacggcct cgcgtttagc acttcactgc acgctcgtca cgatcgctgg
     2101 tgccggatag ccggcgatcg tcacgtcgat ctgccagctg ccggtcgtcg tcggagcggt
     2161 gagcgcgagt gcgccgaccg cggtcccatc ggcgtgctgc acgagcgtga ccggcgcacc
     2221 gacgagctgc ggcacgccat tgatccgcgt gtcgacgagc gcttcgagcg gacgaatgcc
     2281 ggtcggcgcg gtgatgtcga gaccgaccac atcgcccggc gcgagattgc atgccgtccc
     2341 gcacgacagt tttgtgatga gcgtcggcgc acggatcgtc gccgagacct cggtcgggat
     2401 gccgtcgccg agctgcgcgg cgagcaccca ggtcgtctgg tcaggtgcgt tcgggatcgg
     2461 caccgcggtc gtcacctcgc tgtggccgag cacgacctgc gtgcgcgccg gcggcaccgg
     2521 atctggctgc gtgatgccgc cgagcaccgc gtggatcgac acgagttgcg agacctgtcc
     2581 gggcacgctg atgaacacgt gcgcgctgcc ggtcgcaccg gggaggtcgc attgcgagcc
     2641 ctgcgcgcag tcgagcagcg tgaaaccgac cggcggtgga atcgtcgaga acacgacggg
     2701 cgagaccgtc tggaagcccg cgttgacacc cgcgctgatg cgcacgttgc tcgacgactc
     2761 gagcggcgca tagaacgtgg cgatgaagta tgcgtggccg ccgatccgat actcgtcgga
     2821 gatctcgatc ggcacgtcgc tgacaacgcc gtcgacggtg acgacgacat ccggacgtgg
     2881 cgcggacgtc gtgccggaga acgcgacatc gccgtagtcg acgacgacct cgatgaagtc
     2941 agcgccggcg aacagcttcg gcgtcgcggt gtaattgcac gtgcccgaga tgccgaggat
     3001 cggcgagatc ggcgagtcca cggtcatcag cgcgaagcaa tcgccgtctt gcttgagcac
     3061 cgagacgacc ggttgcggat cggtggtgag gccgctcgtg cagccggcga gaagaagaac
     3121 gagtgggtag acgtgcttca tcgggttgcc gtggtgtcgg acgtgggatg cgacgcatcg
     3181 cgcaccagat ccggaaggtt gagttggagc gagaggccga tgtacggcgc gaagatcgtg
     3241 tgtggctgtt cctcgagcag cgtgctgctg cgtttgtcga gcaccgaacc gccgaacgtc
     3301 accgacgtgt atggcagcac gtgcaccgcg gcgccgaaaa acagcggcgg cagcgagccg
     3361 gatccgccgt agcgcatctc cggaccgaac gagctctgat acggcgcgat gccgacctcg
     3421 agcgcgatcg cacggcgcag atccttcgcc gtgattccgt ctcgccactg cggatcgtcg
     3481 accggattcg gcgcgaggtg aatctgcgcg ccgaagtaga acaagccgaa cgagtcgtcg
     3541 ggctgcacca cgctggccca gccgatcatc ggcgtcacgt agctgaagaa ccaggtgcgc
     3601 gacgcgaatg acacgcgcat gccggttggt tcggtcgtcg tcagcgtcac gatgcgttgc
     3661 gaccgcgcga ccagcttgtc ttttgtcgcg cgccagctgt cttcctcgtg ggtcagctcc
     3721 tcgagctgct cgcgcatcgt ctcgatcgcc gccgcgtcga ccttctggca cacgagccac
     3781 tcgccgaggt gctggcggat ggcatcgatc gattgctcgg cttcactgac cgcaaacgtc
     3841 ttcgtcgtgc aggtcgcgcc ggtcgtcgtc agatccacga agttcgcgag ccgcttcatg
     3901 tgcgcacctg ccgttaccag atacgcgctg tcgtctgcgc tccagctcgc ggtgccgagg
     3961 ttcgccatcc gctcgccgag atccttcagc gacacccact ccttgtcgac gcgaatgcga
     4021 cgattgccga gctggatcag atcgtagagc gtgaggccat ctgcgatcgc gagcgtgtcg
     4081 gtcgtcgcgg tcagcacccg cgcctgcgca ccgctcggcg ccttcgacgc ggcgacgcga
     4141 atcgagcggc cgtcgtcgag cagctggatc gctttgacct gcagcttgcc atcggcggtg
     4201 aatagcgcga ccgacgagct cgctccttgc gagcgcacct gtggcaacag cgcgcccgat
     4261 cgcgcgagca gcgccgcgac cgcgtgcgcg aatggatcat tcgcgacttc tgcccacacg
     4321 acggtcacgg tcggagtcat caccgtcgtc ttgtcgtgcc agcgatcgcc catgcgatcg
     4381 atcgcctcga cgatccccgt cgcgctgccg agctccacgc gcgccgcttc acgaagccga
     4441 tcggcgagct cgctcaccct gcccggcacc cgcgccgagt ccgtggcaaa cagcgccttc
     4501 gtgacgcgcg cctcgtagtc gccgagcgcg tcttcgtcgc acgaggcttt gtcgccgcac
     4561 gtaacgatct tcgacgcgac gtcgtcgaag agatcgacga tcgtcgcgtg gtcgagctcc
     4621 tgcgaatgct cgctcgtcac gacgaacagg cagtagccgt agcccgccga gaagaagctg
     4681 cggtcgtacg gcacgagcat attgaactgc cgctcgtcgt cgtggccgcg ctgccacgcc
     4741 gacgacacga gcaccggcgc gcggcggctg tcacccgtgg cattcggaaa gatctcgaac
     4801 gcgcgatgcg gcccactgtc gtagcgcggc atcgcggaag agtcgtcggc ttcatcctcg
     4861 ccttcttcgt catcggccgc cgtcgcggtc gtggtctcga tgcgcaggtc ggcgatcacg
     4921 tcatggcaat cgggaccgtc gtcaccgaac aggctcgacg aaccgcgccg caccacgacc
     4981 gcctgagcgc tgaccaccga cgagcccgcg cgcccggcga tgtagaacga gcggtcggtc
     5041 ggaatctcct gatcgggtcc cgagtcgccg agcgtgatga tcggaatctc gccggccgct
     5101 gactcgcgag gcagcgccag cgcgatcggc aacgcgactg ccatgcagat ggcgaggcgc
     5161 gaggtttcta cgcagtgcat ccgcttgctc tcccatcggg gacggcgcgg cgtctctgtg
     5221 cagtacatcc gcatgatccc ccatcgagaa tcgtcgccgc aggatcgggc caggagcaat
     5281 cgacgggacc tataaagcgc caaccgaacc tgaccagaaa cttggaatca cagcgggatt
     5341 ccggaataaa aaacgaacgt ttgtttttct attgatcgca gacgaccgga tcctctatct
     5401 ctgacgcatg gcccgccgcg cgcttcgcga caaccacgag gagcactgat ggcccgccgc
     5461 gcgcttcgcg acaagccgag tgccgaccgc atcctcaccg cggcggagac actgttcgcc
     5521 aacaagggct acggcgaggt gtcgttgcgc cagctgatcg cggcggcggg cgtgtcgacg
     5581 acggcgttct acgcgcgctt cgactcgaag gcctcggtgc tcgacacgtt gaccgagcga
     5641 ttgttcgcgg agctgcagct tgaagctgcg atggagctgc gcaacgtgcg cgacttcgcg
     5701 acgggtatcg agcgtggcgt cgatatcctg tgtgcgcgct tcggcccgcg caaagcactg
     5761 gtgcgtctcg tcgtcgcgga gtcgggctcg ctagcaccga tgctatctac acggcgcaag
     5821 tcgtacgcga tgcttgcggg ctttctcgcg cactacttca agctcctcgc cgaccgcggc
     5881 cgcatcaccg tcgccgatcc gaatgcactc gcgtgggcgc tcgtcggggc gctcgagatc
     5941 cagatcgttc gctgggcggt gtgggacgag ctcgagctcg accagctgcg cgacgagctg
     6001 ctcgccgtca cacgcgccat cctgccgaag gagaagtcat gacccgtatc gatcgtctga
     6061 tcgccaccgc cgtgctcgcc gccgcggtgc cggccgccgt ggtcgtcgtc ggctgctcct
     6121 ccgatcccgg cacgacggtc gtgatgacga ccgccgacga agcaccggcc tacggcacca
     6181 cgccgttccc gaccgatgcg ctgcgcgaag gcaatcacct cgccacgctg cgcggcctca
     6241 atgcgctcct cgatcgccac gccgacatca tcgctgcaca ctttgccgcg ctcgacgggt
     6301 ttggcgtgcg accgctcgtc gagatcttcg tgaccggtgc gctcgacgag gcgtcgcttc
     6361 ctgcgcacac cgcggcactc gccgatgcgg ccgtcgtcct cgacgtcgat ccggactcgc
     6421 cggagcgcgg ccgcgtgatc gccatggact ggcactacga tgccgagcgc ctcgtgttgc
     6481 aaggcgcgcc aacgagcggc gaggtgctac gtgaaggtac gcgctacgcc gcgttcgtga
     6541 cgagcgcgat caagccgcta cgccgcgcga gcgcattcga tgatctaccg tcgcacgggc
     6601 gctggcagac gaccgcagaa gcactgcaca ccctcgacgc gatggcaggc atcgatgacg
     6661 acatcgtcgg catcaccgtg ttcacgacgc agcatgcgac cgcgccgctg ctcgctgcac
     6721 gtacgatcgt cgagcaactg ccgccgtcgg acctcgtctt cgacgatccg gcgatcatct
     6781 tcaacagcac agctgcgctc gacaaggtga tcggcgtcgc gacgcgcgcc acggacggcc
     6841 cacgcgtcgg actcgagcgc tggggcaatg acaatccgac cggcatcgcg cacgaccacg
     6901 tcggcgtgat cggcaccggc aagatgacga tcgcgcggtt tcgcagcgac gattccaaga
     6961 ccgaccttcc cgacgacgaa acattctccg atccaccgca cgtggtcgcg atggacacga
     7021 ttccggtcac gttcatcctg ccaaaggccg cgatgccgcc cggcggctat ccgatcgtga
     7081 tctacggcca cgggctcggc gcgagtcgcg atcaattgct gtcgtttgcc gagccgctga
     7141 cctcgcgcgg ctacgcgatc gtcggcatcg acatggcggg ccacggctcg cgtttcgatc
     7201 cgaccgatca gctcgcgaac atggcgaatc agctgaccgc gttctccggc gacgccggaa
     7261 cgcgcgatgg cttcggcgac acgaccgggc tcgtcacgca attcgacttc ttcgagggct
     7321 ttctcggcgt gtccgcggtg cgcgattcga tccgccagtc cgcactcgat ctcggccgcg
     7381 tcgcgcaaat gatccggcgc accgatctcg atctctccgc acttgccggg ccgggcagcg
     7441 cgaacccgaa gctcgatacc cggcacatcg cgtatctcgg cgagtcgttc ggcaccgtcg
     7501 tcggcagcgt gttctcggca atcgagccga acatcgatct ctacgtcctc gacgtccccg
     7561 gcggcggcat cctcgatcac atcctgactt cgagccctga gatcgcggcg actgcagttc
     7621 cgctcatcga cagcatcctc ggtccacgcc agcgcgtcga tcgcttcaat ccgctgatcg
     7681 gcctcatgca gtcggtcatc gacggcgccg acccactctc gtacgcgcct cacgtactcc
     7741 gcgatcgctt cgcgatcaac ggtaccgtcc tcggtccgcg cagcgtcgtc tgcctcgaag
     7801 tgctcggcga tcaagtgctc gcgaacgtcg gcaccgattc actcgcgcgc gagctcggcc
     7861 tcgacgtgct cgtgccgaac ctcgcaccgc ccgacggtct cgtcccgatc gcctcgcccg
     7921 ccgccggcaa tcgcgacggc atgaccgctg tcctcgtgca gtactcgccg gcgacccacg
     7981 gcgcgaactg gagcgcggag cacggcgtgt tgcgctacct gccgggattc ccgttcgacg
     8041 acgagaatca gtttccgaag ctgcccgcgc cggtcacgat ccggaacccg atctacgaga
     8101 cgcacgcgca ggtcgccgag atcctcgaga catacttcgc cggtgagtca cctcgcgtgc
     8161 gcagcacgct cgcaccggtc gccgacttcg acgacgacgg cacgcccgac gccaccgacc
     8221 cggcgccgta cgatccggcg cagcgctagc tggcttgcgc taagagcctt tgcgaatctc
     8281 gccggcgacc tgatcgagct cgttgctgcc ttcggctctc gtgccgtccc atggcagcgc
     8341 gcgtccgaga tcgtcgagtc cgaacagctc ggcgatcgcc gcgagctcgc gcggcttgaa
     8401 cgtcttcgtc cgatacggtg acgcgagcgc aacggcgagc acttcgctct tgcgaccgag
     8461 caccggatcc ggcggcatcc acgagaacaa cgcggcgccg tcgatgtcgg cttcggaatc
     8521 ggcagcgtca tcggggctcg cgagtcgtac gagcacgtcg tcgctcatgc cgccgccatc
     8581 gacgttgtcg acgtgtaccg aaatcacgcg acgtgacacg ccttgcgttg cgcgctcgat
     8641 ggtaacgagc gccgatggtg caccacgagt atccaccgtc gcgcacagtg agccgatcgg
     8701 tagcggctcg acgtcgagct tcttgtcctt cgggaacggt tggccgagct cgatcacacc
     8761 catatgcggg acgcgcccca tcgaccacga cgacaccggg agcgttttcg aacagtcgag
     8821 cgtgagcgcg atcgcgagcg gttcacccgc cttgcgcaac agctccgcac tcgagaacgc
     8881 gatggtctgc gtgccgttcg tgaccagtgt gcacgggccg accagctgaa gcgtaggggc
     8941 cgttacgacg agtggcgcga ccgctcgaag ctcgttcgcc tgttcacgcg tcccacgacc
     9001 catcgaggcg agactggcac ggcaagaagg tcattctcaa ctggcaaaga cgtgcgcagc
     9061 gtgcgtgggc gcgcgcccga tgtacgggca tgcgaggtgc acggtaactg cccatgagcc
     9121 agggcaggac acgacgttat cgaatcgtgg ttggcgtcga tttgacggag tactcgcaca
     9181 tcgtgatcga ccacgctctt gatcaagctg cgcatcatcg cgcgcccgag cttcacttcg
     9241 tgcacgtgaa agagagccgc aggcacgacg tcaacacgct cggcgagcgg ctgtccgcgg
     9301 cggtgtatcc atcgctgcaa gtcttcaacg agtacggcaa ggattggcgc gcgcgcctgc
     9361 acatacgcaa ggggcgaccc gacgagcaaa tcacgatgct agcggccgat atcctcgccg
     9421 acatgatcgt gatcggtgac ttcggtctgc accgcaaggg catgccgaag cgcatcctga
     9481 cccacgcgat ctgcccgacg ctggtcgtcg ggatgccgaa gctgctcgac accaggcagt
     9541 gcggcgagtg tgccgcgctg cgcgaagaca gcgaaggcac acggtggttc tgcgacgatc
     9601 accgcgtcaa acagaacgcc gtatcgccga tgacgacgtg gagcaatggc agcttgatgc
     9661 aatgagcccg tcggtggcat cgtgggtcta tgcacgcgat gcccgccacg gcagaggagc
     9721 cacatgcacc gcctccgccg cgctcgctgc ggcgcgcgct cgtcacgacg ttccgcggct
     9781 tgttgcgcct ctacttccgg cgcatcgagc gagtcggcga ggcgccgggc gctgaaacgc
     9841 gagggcgcat gttcgtctcg aatcaccaca acgcgctgat cgatccggtg ctcgtgctca
     9901 ccgatgcgga atgcgagatt tcgccgatcg cgaagtcgac gctgtggtcg attcccgggc
     9961 tcaagtatct cctcgacagt gccggcgcgg tgccgctgta tcgccgcaag gacgatccga
    10021 acaaggcgac gtcggcgaac gacgcggtgt tcgcgaagat tgcttcgcac ctcggcggcg
    10081 gcggcaacat cctgatcttt cccgaaggca cgagccacag cgagccgcat ctcgcgccgc
    10141 tgcgcaccgg cgccgcgcgc atgttgatgg cagccggcgc atacggctca ccgctcacat
    10201 tccaggccgt cgcgctcgaa ttcgacgaga aggatcgctt tcgttcgcgc tgcctcgtgc
    10261 tctgggggcc cgtgcgcaag ctcgccgatg tcggcggcag cggagaagac cgcgtccgcg
    10321 cgatcaccgc gcagatggac accgatctgc gcgagctcct cgtcgagggc gacacgcacg
    10381 acgaacgcat gttgatcgca cgcgtcgccg agatgctcgc gaacgacgcc ggtgatggct
    10441 cgctgtcggg ctggagcacg atcggccgcc aggtcgagct tgccggccgc tcgcttcgcg
    10501 aagctgatcc ggcgctcgtc gcccacgtcc gcgagaaagt cgacgcgtac tacgccgagc
    10561 tcgcgcgctt cggccgcgtc gatgcgcagc tgtcggcgac ccgcgccgcc gtgaagaagc
    10621 gcccggcgcg gtggctgcgt cgcgccgcac tcgcaccgct cgccgttccc gggttcgcgc
    10681 tctacgcggt cccctacctc atcccgcgcc tggtcgcgcg cacgagcgat ccggacgccg
    10741 tctcgacggt gaagctcgcg accgcacttg ttgtctatcc cctgtgggct gcgggcctgg
    10801 tcgggctctc gctcgtgctc gtcccgcctc cgctctcgct cgtcgccgcg ggcctcgtgc
    10861 tcgtgtcacc gttcgcggcg ctgcgctggc tcgacgcgta ctgggagcgc acgcccgagc
    10921 cgactgccga cgaattggcg cagctcgccc gtctccgtat cgcagctcgc accgcgatcg
    10981 acgaagcgcg tagtcgtctc taacccgtct ggcacgtgga cacaccgcat gtcaccacgg
    11041 ttgcatgcgc gcattgctcg tcttgctagc gctcgctgcg ccggcctccg cagcagcttc
    11101 gtccgaagac gactggctca ccgggctcac ggatcgtgtc gtcgacgatc tcaccgccgg
    11161 caagtcgctc aatgtcctcg tccacgtccc gctctgcgac aacacgatca tcgcgtgcgg
    11221 taacgcgaag ctcggcgacg gcgatactcc atcgacgaac ctctactggt cgacgacgcc
    11281 cggcttcggc caatggttcc agcgcaaggg cagcggctgg acgcgggtgt cggtcgagac
    11341 gaaggacacc ggcgacaagg acgtgctcga gaagcacatc tatcggcgca cgatcgcgac
    11401 gccggccgca tggcgcaagc gcggcgcgcc gaagcagttc acgctcgagg tcgagattcg
    11461 cgcgtggcgt ggctcggcga tcgatcgcgc actcgccgcg tacgcgaacg acgtctcggg
    11521 caaaggctcg agcgcgcagc tcgtcgcgtt cgtcggtcac aatcgattga tggacctgga
    11581 cacgttcgcg tggcccgagc ccggcaagga cgtcaaaggt gcgatcgcga tcgcgtgcga
    11641 taccgcgccg tacatgaaga agcaggtgtc ggcggcgacg cgcgtcccgc tgctcatgac
    11701 cgccgatttg ctgttcgcga acgccgcgcc actcgaagca gccgtgctcg cattcgcgcg
    11761 cggcggcggc tacgcgcaga tccgcaagga cgccgcgact gcatacgccg gcgtgcgcaa
    11821 acgtcccgtc gagaaagtga tcggcgcgtt cacgaatccc ggcgatcgcc gctggtgatc
    11881 actcgtcgcg gctgccgatc aacggcaagc cggatcgcac gtcggcatcg ttcgcggcgg
    11941 gcttgccacc gcgcgacagg atcctgagcg agcgcaagat ctcgtcggcc gccggccact
    12001 cgggcgacgc gcaacgcacg gtcacgatca agccgccatc gagcgcgaga tcgtgcggcg
    12061 ccatcgcgaa cacgtagacg tacggtagcg gcgccttcgc gacgccggcc ggccgcacga
    12121 cttcgagctc cgcgcgatag ccgctcgcgc ccggcagtgc gattggcatc gcaggcgaca
    12181 cgcgagcacc cggctctcgc gcgctctcga tcgcgctcgc cgccttctct tccaggatgc
    12241 cgtcgcgatc gatcacgagc gcggcgtgaa acaccgcgac ttcgagctca ccgaccgtct
    12301 tgccatcgcg ccgctcgctg cacttcacga ggccggcgcc tggatggtcg atcacctcga
    12361 cctgttcggg cgtttcgatc tcgaagccga ttctccgaga cgtcgtcggg agctggcgat
    12421 aggacatggc ctattgcctt gagtttgttg cgggagcgcc gggagtggca agcaacaact
    12481 gcaggccacc gagattgctc gaagaactac gacttcggca gccgcgccgc gcgcttcagc
    12541 atctcgatca tctggcggcg atagccctcg gcgtcctggc ccttcgcgcc ctcggcgagc
    12601 tgcgtgacgg tcgcccacga gatgttgccg cgatccttcg cctcgcggag catcatgccg
    12661 aagcccgcaa cggccgcggc ccagcgaaag tcgatcgacg tcttcgccat ctcgaccttg
    12721 gactgcttca ccgcttgcga cagcagcttg ctctggttgc cgtccggcgc cttgtagcga
    12781 atcttcaccg tcatcagctc gtccgcgttg ccaccggtcg gctgcgcttt gccctggtac
    12841 ttgaggttgt cgaccttgcc gcccggcact tcgacgcccg ccggcacgac ctcgtagaga
    12901 gcggtgaccg agtgaccggc gccgatgtca ccggcgtctt tcttgtcgtc gttgaagtct
    12961 tcatcccgca tcaggcggtt ctcgtaaccg atgaggcgat agcccacgac ggtctgcggg
    13021 ttgaactcaa cctggaactt cacgtccttc gcgacggtga cgagcgtcgc gcccgcttcc
    13081 ttgacgagca ccttgcgcgc ttcttcgatc gtgtcgatgt acgcgtagtt gccgttgccg
    13141 cgatccgcga gcttctccat cgtcgagtcc ttcaagttgc ccatgccgaa gccgagcacg
    13201 ctgaggaaca cgccgtgctc gcgcttctct tcgatgagac gcgtcaggtc gccttcgctc
    13261 gtcgtgccga cgttgaagtc accgtcggtc gcgaggatca cgcggttgat gccgcccttg
    13321 atgaagttct tgttcgcgat ctcgtacgcg agcttgatgc ccgccgcgcc gttcgtcgag
    13381 ccaccggcct cgagcgcctg gattgcctga cggatcgcat ccttctcctt gccggtcgtc
    13441 gacggcagcg cgaggcccga cgcacccgcg tagacgacga tcgcgatgcg gtcgttgtcg
    13501 cgcatgttct cgacgagcag gttcatcgcg cgcttcacga gcggcagctt gttcgcgacc
    13561 atcatcgagc ccgacacgtc gagcaagaag acgaggttac gcgccggcac cttgtcgtcg
    13621 ttgatctcgg gcgccttgag accgatgcgc acgagctgga acttcgagtt gaacgggctc
    13681 gggccgacct cggtcgtgat cgaaaacggc tggcccttct gcggcgccgg caggtcgtag
    13741 tggaagtagt tgatcatctc ttcgatgcgc accgcgtcct tcggtggcag ctggccgtca
    13801 cgaatgaagc ggcgcaggtt cgcgtacgac gcggtgtcga cgtccgacga gaacgtcgaa
    13861 agcggcttct gatcgacgcg caggaacggg ttgtcgtcga tgcgcgagta cgcctcggtg
    13921 ttcatcgacg ggtcttgctc gacctgcagc ggctgccggc gcgacatccg accgcccgcc
    13981 ataccgaccg acggcgacga cggcgacggc gcaaccgtca tcggaggagg cgccggcggg
    14041 cccatgtggc gcgacacgtc ggcggtcttt tcggccggct tgttgtcagc cttcggccgt
    14101 tccttcgcga cgtccttcgc gacgacctct tcattcttct tgtcgacggg cgcgaccgcg
    14161 agcgcgagct tcacagacat
//
LOCUS       DASUFX010000753         6194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1926259, whole genome shotgun sequence.
ACCESSION   DASUFX010000753 DASUFX010000000
VERSION     DASUFX010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926259"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFV99_30515"
     CDS             <1..269
                     /locus_tag="VFV99_30515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063766.1"
                     /translation="RFEANFALLCPTSCSAGLDQTPTERQLAEQRDSAKLKGAVGVSM
                     MALGGAVIVGGVVWVVLNRPHRVLPKMEVTPTSGGASASVGWQF"
     gene            complement(266..1483)
                     /locus_tag="VFV99_30520"
     CDS             complement(266..1483)
                     /locus_tag="VFV99_30520"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5063767.1"
                     /translation="MQEVPASPIPRLVGARSLNTFGRAVLSATVLWELYDRTGSKLVL
                     AAVGLVQVIPVVLLFVPVGRLVDKSDRRTLTTACAALAGVVGVALAVASALAAPVAIY
                     LALLLGWGCITAVMAPASVSLIPLIIKREDLIRVNRISSSLQELAAITGPALAGLALN
                     VFVPAAVYSAIAVTGLASAALYRSLPKPRAFEPSAGAARRDWRVGLRFISRSPLLLSA
                     LTLDMFAVLFAGVTALLPVVASDILQVGPIGFGVLRAAQSAGAVAMAVIGGRMPPWQR
                     PGRALLVVVALFGACTIGFGLSTSFPLSVLLLFLCGALDNISVVIRLTLEQMVVPDEI
                     RGRVSAVHFVFIGMSNELGAAESGAAAWLIGTVPAIVAGGAVAMIVAGIVAVKWRELA
                     AMPPLSDLKPLDL"
     gene            1532..2320
                     /locus_tag="VFV99_30525"
     CDS             1532..2320
                     /locus_tag="VFV99_30525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063768.1"
                     /translation="MTDRAVHYAVRGLVAATLACAIAAELAARVVLSHGSGTYFGLLV
                     FLPLLAAPGALVWRFPQPKYLTLWALAGWLATLVWSIAGAPYQYERALPHWLLVSTPV
                     WMAVALVQFVAPLVAALAVRTDAIAPERALFALRLRRIVVLVFAIATIVVVTCFAFAG
                     SEGMSVAVYTGAVIGPGFAVQWFPNRLTAVMWSVLCLPFAGLGIWLWATFGTAEHWAV
                     HVVVAGLGTIYVLVVLALPIICIVTPDRSVTDSSARARYRSRNP"
     gene            complement(2274..3662)
                     /locus_tag="VFV99_30530"
     CDS             complement(2274..3662)
                     /locus_tag="VFV99_30530"
                     /inference="COORDINATES: protein motif:HMM:NF039689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6209 family protein"
                     /protein_id="HEX5063769.1"
                     /translation="MLLSVVLVGCVDAAEETTTAPAPLVGVDGSHDAADRNCNIVLRN
                     LERPWTGFTWETNGSSWVWAGTIEISDAAAAEGLVPRLLYQYGSDPTWHEVAATPAQQ
                     PGTPGFSRYTVRIDQDLPGPGMSGTALANARVQVVPMLRMPQGGRLFDHNRNPGDVDN
                     YVMTSPDFAVWGNDAVCAPPAGPQRARLVFDADWTEHREGVLAPGGEVTIVYAQTRLN
                     QCKQTQNGYELWDITAHVRFEPGGQLLGASVRAGTATMTVPSDARRAVVWFESTSVSG
                     CHYWDSNYGNNYVFDAATPPQWVGNMSSLMTRDTSGDICGGTPAQQGFAFDTWTRERA
                     AITNLCFQVYQPGMTDHDDPDLWQKLDVSVRWHGEGQPAVWQSRSINFDRRVGNDARY
                     AMSWRDLDPFRPYHCPDVATTTTADGMYVQARLEYIVVVNGYELRPVPGATFSGTFTD
                     YANDTWRAQNCP"
     gene            complement(3780..5102)
                     /locus_tag="VFV99_30535"
     CDS             complement(3780..5102)
                     /locus_tag="VFV99_30535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063770.1"
                     /translation="MPSPRYPKPGTVVLGKYRVEAMIGEGGMGAVVKAYHIDLEEPVA
                     IKVLLPEMLDRQEIVQRFLREAKAAVKLKGEHVARVLDVGRLDADVDGNFEGVPYIVM
                     EYLEGSDLNAIVKHYGPQHPTMAVDLMLQACEAIAEAHSIGIVHRDIKASNFFITMHD
                     GAPPTLKVLDFGIATTPKGTSELTDGGQVVGTPAYMAPEQMRSSRLADARSDVWSMGV
                     VLYEMLEGARPFRADAYSDLCLRVGMDPPEPMVQQAVPEGLRAVVMKCLEKTVERRYQ
                     SVAELAFDLMSFASDSVLARASVEQCARMLSRRTRPVDVSRMPDDVTPASNVPPRLTP
                     PAFPRLSAPAQSPISSSHSTPAESKGQVGAPSERKRHALVGVASFVVAGALTGVVALL
                     LTRGSDEPVVHDDTPSEIQMRAELTPPDAAPDATPDAATEAPFDAPPD"
     gene            complement(5139..>6194)
                     /locus_tag="VFV99_30540"
     CDS             complement(5139..>6194)
                     /locus_tag="VFV99_30540"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5063771.1"
                     /translation="YWIDVADFDGFRIDTVKHIDRPEVNRNVRGFWGEFADRMRKKAH
                     DLQKQNFFIFGEAFDGKDDLIGSYTWGGTDTAGSFGRLDSVFYFSQKYRGIDAVFANA
                     GPTKNLECLYNSRIGRNGTDAWCMQNGYPQGPDYQNTPHATPEQGGIGLAPTQVLVNF
                     LDNHDLPRFMFEKSDPAIQRVALMYLMTWDGIPCVYYGTEQGFAGGVDPKNREDMFRG
                     NEALGFAPWATDHEYYKLVHDLIQLRKDNVALRRGQVTPVYSTTVATGRDAGIFGFER
                     SVPGDQTALVVLNASPQMSESCASPGAGCMKTSLPAGSVLTDIMPGSDGATFTVKSDG
                     TVAVTVAARSGRVLVKK"
BASE COUNT         1022 a         2096 c         2039 g         1037 t
ORIGIN      
        1 atcgcttcga ggcgaacttc gcgctcttgt gcccgacgag ctgctcggcc ggcctcgacc
       61 agacgccgac cgaacgccag ctcgccgagc aacgcgatag cgcaaagcta aagggcgctg
      121 tcggtgtgtc gatgatggcg ctcggcggtg cggtcatcgt cggcggcgtc gtgtgggtcg
      181 tcctcaatcg gccgcatcgc gtgctgccga agatggaagt cacgccgacg agcggtggtg
      241 cgagcgcgtc ggtgggctgg cagttctaga ggtcgagtgg cttgaggtcc gagagcggtg
      301 gcatcgcggc cagctcgcgc cacttcaccg cgacgatacc ggcgacgatc atcgctacgg
      361 caccgccagc gacgatcgcg ggcaccgtgc cgatcagcca cgcagctgcg ccggactccg
      421 cggcaccgag ctcgttcgac atcccgatga acacgaagtg cacggccgag acgcgaccgc
      481 ggatctcgtc gggcacgacc atctgctcga gcgtgaggcg gatgacgacg ctgatgttgt
      541 cgagcgcgcc gcacagaaac agcaacagga ccgacagcgg gaacgacgtc gacaggccaa
      601 agccgatcgt gcacgcgccg aacagcgcga cgacgacgag caacgcgcga ccgggtcgct
      661 gccacggcgg catccgtccg ccgatgacag ccatcgcgac cgcgccggct gattgcgccg
      721 cgcgcaacac gccgaacccg atcgggccga cctgcaagat gtcgctcgcg accaccggaa
      781 gcagtgcggt gacgcccgcg aacagcacgg cgaacatgtc gagcgtgagc gccgacagca
      841 gcagcggcga gcgagagatg aagcgcaacc cgacgcgcca gtcgcggcgc gcggcacccg
      901 cgctcggctc gaatgcgcgc ggctttggca gtgaccgata cagtgccgcc gaagcgaggc
      961 ctgtcactgc aatcgcggag tagaccgctg caggaacgaa cacgttgagc gccaaaccgg
     1021 cgagtgccgg tccggtgatc gcggcgagct cttgcaagct cgagctgatc cggttcacgc
     1081 ggatcagatc ctcgcgtttg atgatgagcg gaatcagcga cacgctcgcc ggcgccatca
     1141 ccgcggtgat gcagccccag ccgagcaaga gcgcgagata gatcgcgacc ggtgccgcaa
     1201 gcgccgatgc gactgcgagc gcaacaccga ctacacccgc gagcgccgcg catgcggtgg
     1261 tcagcgtccg ccgatcgctc ttgtcgacga gtcggccgac cggcacgaac aacagaacga
     1321 ccgggatgac ctggaccaga ccgacagcgg ccaagaccag cttgctgccc gttcgatcat
     1381 agagctccca caggaccgtc gcgctaagca ccgcgcggcc gaaggtattt agcgagcgcg
     1441 ctccgaccag acgtgggatc gggctcgcgg ggacttcttg cactccgatg cgatgctacc
     1501 gctttgacct cggttgcggc atgcttctac ggtgacggat cgtgcggttc actatgcggt
     1561 gcgtgggctc gtcgcggcca cgctcgcatg cgcgatcgca gccgagctcg ctgcccgagt
     1621 cgtgctcagt cacggatccg gaacctactt cggactgctc gtgttcttgc cgctgctcgc
     1681 cgcacccggc gcgctcgtgt ggcggttccc gcagccgaag tacctcacgt tgtgggcgct
     1741 cgccggatgg ctcgcaacac tcgtgtggtc gatcgccggt gcgccgtatc agtacgagcg
     1801 cgcgttgccg cactggctct tggtgagtac gccggtgtgg atggcggtcg cgctcgtgca
     1861 gtttgtcgcg ccgctcgtcg cagcgcttgc agttcgcacg gacgcgatcg caccggagcg
     1921 tgcgctgttc gcgttgcgac ttcgccgcat cgtcgtgctc gtatttgcga tcgcgacgat
     1981 cgtggtcgtc acgtgcttcg cattcgccgg cagcgaaggc atgtcggtcg cggtctacac
     2041 cggtgccgtg atcggccctg gcttcgcggt gcaatggttt ccgaaccggc tcaccgccgt
     2101 gatgtggagc gtgctgtgcc tgccgttcgc cgggctcggc atctggctat gggccacgtt
     2161 cggcaccgcc gagcactggg cagtgcacgt cgtcgtcgcc ggactcggga cgatctacgt
     2221 gctcgtggtg ctcgcactgc cgatcatctg catcgtgacg ccggaccgct cggttacgga
     2281 cagttctgcg cgcgccaggt atcgttcgcg taatccgtga acgtgcccga gaacgtggcg
     2341 cctggcacgg gccgtagctc gtagccgttg acgacgacga tgtactcgag ccgcgcttgc
     2401 acgtacatgc catcggcggt cgtcgtcgtc gcgacgtcgg ggcagtgata cggacggaac
     2461 ggatcgagat cacgccacga catcgcgtag cgcgcatcgt tgccaacgcg ccgatcgaag
     2521 ttgatcgatc gcgactgcca gaccgcgggc tggccttcgc cgtgccagcg cacgctgacg
     2581 tcgagcttct gccacaggtc cggatcgtcg tgatcggtca tgccgggctg atagacttga
     2641 aagcacaagt tcgtgatcgc ggcgcgctcg cgcgtccacg tgtcgaacgc gaagccctgc
     2701 tgtgcaggcg tgccgccgca gatgtcgccg ctcgtgtcgc gcgtcatcag cgagctcatg
     2761 ttgccgaccc attgcggtgg cgtcgcggca tcgaagacgt agttgttgcc gtagttcgag
     2821 tcccagtagt gacaacccga gacgctcgtc gattcgaacc acacgaccgc gcgccgcgca
     2881 tcgctcggga ccgtcatcgt cgcagtgccc gcgcgcacgc tcgcaccgag cagctgacca
     2941 cccggctcga accgaacgtg cgcggtgatg tcccacagct cgtagccgtt ctgcgtctgc
     3001 ttgcactgat tcaagcgcgt ctgcgcgtag acgatcgtta cctcgccgcc cggcgcgagc
     3061 acgccttcgc gatgctcggt ccaatcggcg tcgaacacga gccgcgcgcg ctgcggaccg
     3121 gcaggcggcg cacacaccgc gtcgtttccc cagaccgcga agtcgggcga cgtcatgacg
     3181 tagttgtcga cgtcaccggg attgcgattg tgatcgaaca gtcggccgcc ttgcggcatg
     3241 cgcagcatcg gcaccacctg cacgcgcgcg ttcgcgagcg ccgtgcccga catgcccggg
     3301 cccggcagat cctgatcgat gcggaccgtg tagcgcgaga agcccggcgt tcccggctgc
     3361 tgcgccggtg ttgccgcgac ctcgtgccac gtcggatcgg aaccgtattg atagagcagc
     3421 ctcgggacga ggccctcggc agccgctgca tcggaaatct cgatcgtgcc ggcccacacc
     3481 cacgacgaac cgttcgtctc ccacgtgaag cccgtccacg gccgctcgag gttgcgcagc
     3541 acgatattgc aattgcgatc ggctgcatcg tggctgccgt cgacgccgac gagcggtgcg
     3601 ggagccgtcg tcgtctcctc cgcagcatcg acacaaccga cgagcacgac ggacaagagc
     3661 acggagcgaa gcatcgtgcc cgcgcgacgt gcggcgtacg tgccagatcc gtgtcgtgcg
     3721 gctgcgagta cttagccgcc gcgaggcccg gtcatgttgc gttcggcatc gcgccgtggc
     3781 tagtccggtg gcgcgtcgaa cggagcctcc gtcgcggcat ccggcgtggc atccggcgcg
     3841 gcatccggcg gcgtgagctc ggcgcgcatc tggatctcgc tcggcgtatc gtcgtgcacg
     3901 accggctcgt cggaaccgcg ggtgaggagc aacgcgacca caccggtgag cgcgcccgcg
     3961 accacgaagc tcgcgacgcc gaccagcgcg tgacgcttgc gctcgctcgg cgctccgacc
     4021 tgccctttgc tctccgccgg tgtcgagtgc gacgacgaaa tcggcgactg cgccggtgct
     4081 gacaaccgcg gaaacgcggg cggcgtgaga cgcggcggca cgttcgacgc cggcgtcaca
     4141 tcatccggca tgcgcgacac gtcgaccggg cgcgtgcgcc ggctcagcat gcgcgcgcac
     4201 tgttcgaccg acgcgcgcgc gagcaccgaa tccgatgcga acgacatcaa gtcgaacgcg
     4261 agctcggcga ccgactgata gcggcgctcg acggtcttct cgaggcactt catcacgaca
     4321 gcgcgcaggc cctccggcac cgcctgctgc accatcggct ccggcggatc cataccgacg
     4381 cgcaggcaga ggtccgagta cgcgtccgcg cggaacgggc gcgcaccctc gagcatctcg
     4441 tagagcacga cgcccatcga ccacacgtcg ctacgcgcat cggcgagtcg cgacgaccgc
     4501 atctgctccg gtgccatgta cgcaggcgtg ccgacgacct ggccgccatc cgtcagctcg
     4561 ctcgtgccct tcggcgtcgt cgcgatgccg aagtcgagca ccttgagcgt cggcggtgca
     4621 ccgtcgtgca tcgtgatgaa gaagttcgac gccttgatgt cgcgatggac gatgccgatc
     4681 gagtgcgcct ctgcgatcgc ttcgcacgcc tgcaacatca gatcgacggc catcgtcgga
     4741 tgctgcggac cgtagtgctt gacgatcgcg ttgaggtcgg agccctcgag gtactccatc
     4801 acgatgtacg gcacgccctc gaagttgccg tcgacatcgg catcgagccg gccgacatcg
     4861 agcacgcgcg cgacgtgctc gcccttgagc ttcaccgccg cttttgcctc gcgcagaaac
     4921 cgctgcacga tctcctggcg atcgagcatc tcgggcaaca gcactttgat cgcgacgggc
     4981 tcctcgagat cgatgtggta cgccttgacc acggcgccca tcccgccttc gccgatcatc
     5041 gcctcgacgc gatacttccc gagaacgacg gttcccggtt tcggatagcg cgggctcggc
     5101 acgcgctccc agtttagtcc aacgcggaag tggcagcgct acttcttgac cagaacacgt
     5161 cctgaccgag ccgcgacggt caccgcaacg gttccgtcac tcttcacagt gaacgtggcg
     5221 ccgtcgctcc ccggcatgat gtcggtcagg accgagcccg ctggcagcga cgtcttcatg
     5281 cagcccgcac cgggcgacgc gcagctctcg ctcatctgcg gactcgcgtt cagcacgacg
     5341 agcgccgtct gatcacccgg gacgctgcgc tcgaatccga agatgccggc atcgcgcccc
     5401 gtcgccaccg tcgtcgaata caccggtgtg acctggccgc ggcgcagcgc gacgttgtcc
     5461 ttgcgcagct ggatgaggtc gtggacgagc ttgtagtact cgtggtcggt ggcccacggc
     5521 gcgaacccga gtgcctcgtt gccgcggaac atgtcctcgc ggtttttcgg atcgacgccg
     5581 ccggcgaagc cctgctcggt gccgtagtag acgcacggga tgccgtccca ggtcatcagg
     5641 tacatgagcg cgacgcgctg gatcgccgga tcgctcttct cgaacatgaa ccgcggcaga
     5701 tcgtggttgt cgaggaagtt gacgagcacc tgcgtcggcg cgagaccgat gccgccctgc
     5761 tccggcgttg cgtgcggcgt gttctggtag tcggggccct gcggatagcc gttctgcatg
     5821 caccacgcgt cggtcccgtt gcggccgatg cgcgagttgt agaggcactc gagattcttc
     5881 gtcgggccgg cgttcgcgaa caccgcgtcg atgccgcggt acttctgcga gaagtagaac
     5941 accgagtcga gccgaccgaa cgagcccgcg gtgtcggtgc cgccccacgt gtagctgccg
     6001 atcagatcgt ccttgccatc gaacgcttca ccgaagatga agaagttttg cttctggaga
     6061 tcgtgcgctt tcttgcgcat gcgatcggcg aactcgcccc agaaaccgcg cacgttgcga
     6121 ttcacttcgg gacggtcgat gtgcttgacg gtgtcgatgc ggaagccgtc gaagtcggcg
     6181 acgtcgatcc agta
//
LOCUS       DASUFX010000754         8502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1651191, whole genome shotgun sequence.
ACCESSION   DASUFX010000754 DASUFX010000000
VERSION     DASUFX010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651191"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFV99_30545"
     CDS             complement(<1..273)
                     /locus_tag="VFV99_30545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063772.1"
                     /translation="MRHVAIFVVTLVLAGCPRSGGDTTPARPATPKEVVAAARATVEQ
                     WRQAYEVRSFDALAKLYAHDIDLVVIQDGVPIIGWSSVEIMLKDRLA"
     gene            347..478
                     /locus_tag="VFV99_30550"
     CDS             347..478
                     /locus_tag="VFV99_30550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063773.1"
                     /translation="MALEHLENDERQATTTILLTAIMSVIITGLIVAAVLSTRACSV"
     gene            489..1766
                     /gene="eno"
                     /locus_tag="VFV99_30555"
     CDS             489..1766
                     /gene="eno"
                     /locus_tag="VFV99_30555"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5063774.1"
                     /translation="MTEIVWLHAREVLDSRGNPTVEAEVGLDGGVLGRAIVPSGASTG
                     EHEALELRDGDAKRYLGKGVTKAVDHVRDVIAPTLIGMDASDQAAVDKALIQLDGTPM
                     KSNLGANAILSVSMAVARAASMAHELPLYRYLGGVGAVTLPVPLMNIINGGAHADNNL
                     DIQEFMIAPAGFDRFEDALRAGVEVFHNLKKLLSSRGKATNVGDEGGFAPSLDSGDEA
                     LQLVLEAIDKANYEPGKQIFLALDVAASELFDKKTGMYTWEGKQRKAADLINIYAGWA
                     EKYPLVSVEDGLAEDDWDGWKDLTDRLDGKVQLVGDDLFVTQTARLKRGIDNGIANSI
                     LVKVNQIGSLTETLDAVRTAQHAGYTAIISHRSGESEDSFIADLAVATNAGQIKTGSA
                     SRSDRIAKYNQLLRICDQLGDEARFAGAGVFKR"
     gene            1766..2101
                     /locus_tag="VFV99_30560"
     CDS             1766..2101
                     /locus_tag="VFV99_30560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063775.1"
                     /translation="MPPSGYSFKSNLTFAQIRARLAELGHGTWEERESDIYGSYIRGQ
                     AFGVWMRIYDGVGCTSELGTYDPKGFMLLDYARRALADISAAHDKRVREELLPALDVT
                     EWHEDEGND"
     gene            complement(2067..3035)
                     /locus_tag="VFV99_30565"
     CDS             complement(2067..3035)
                     /locus_tag="VFV99_30565"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5063776.1"
                     /translation="MNRIARAWRAWVALMDRREPATALALVRIGIALVLLVDYAWIAH
                     AGLIDPLWSRFPDGYATGFTGVADTLGLSAFGLWAIAMAAIACLLFGIATPIACVGFV
                     AVSIQQAALAPDSESGVDQLFRIVLLVLALSRCNARWSVDAWLLRKLGRPMSALVPAW
                     PRYLILFQLLWVYFSGGQNKSSHAWDPLGGFTALGNALMDPHNGRLSTDFISTIYPLT
                     RVATALTITFELTALFYLLWLYFAATAERPGRLRRFANRARLRWIWFSLFVLFEVGIA
                     VGLRLGSFPYGMLAMWPVLLLPDELERLAERIAQRLSRSPRPRATP"
     gene            complement(3032..3577)
                     /locus_tag="VFV99_30570"
     CDS             complement(3032..3577)
                     /locus_tag="VFV99_30570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063777.1"
                     /translation="MKRWPEIRAGLILLAIFFALVDGCPLPPADQTPDWEKGFVEPIR
                     DVQHVVLKPVAWIGRTLQVGQRWSLYQSPSTERYRLWVEGEDMQGRWQLLFRASDPEH
                     TDDAAFIDTARMRGSYDPTDRLPGQYAVLARYLTQRVLDRHPELVAARMRLEKVHITS
                     DGVVPSGQFIGQHVRLRNGPP"
     gene            complement(3596..3886)
                     /locus_tag="VFV99_30575"
     CDS             complement(3596..3886)
                     /locus_tag="VFV99_30575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063778.1"
                     /translation="MSADLAEERLMLVSLRSQARRSIAAGALLVVSLAPAMWLGAMTN
                     DVGLLAATIYAPFAFVWGARCLWSGISMYSVASFKLRRLHARDTGLPPARLV"
     gene            complement(3902..4654)
                     /locus_tag="VFV99_30580"
     CDS             complement(3902..4654)
                     /locus_tag="VFV99_30580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5063779.1"
                     /translation="MLTETEITREPSAVPPIATGSGPTQVGTRATGQAPDVFGNIFIG
                     IAGMIGAGKSTLAAELGKHLGIDCYYEPVADNEYLADFYRDTARYSFAMQVYLLNRRF
                     QQHQEIIWRGKSAVQDRTIYEDSIFAKMLAKTGLMDERDYRTYVELFRNMSNFMCKPS
                     VIIFLDVSPEASAERIRMRSRDVEAKIELGYLQALYEGYQEFIASISKVIPVIRVDYA
                     RFATAEEMAEVIKREYLDASFLRQVTRFDPAR"
     gene            4701..5267
                     /locus_tag="VFV99_30585"
     CDS             4701..5267
                     /locus_tag="VFV99_30585"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063780.1"
                     /translation="MRKVAIGLVLATLVSGCVVRTHGRVNPLASMLVTGIAVAAVAST
                     VQVSNPPPPTVEVGYYGYARPGYVWVNGYHRWNGNAWVWTNGYYQAERAGYYWVQPSW
                     ELRGDQYVWVDGYWAEPRAGYVYVDGYYDYRGSGYYWTPGRWEADRPGYTYVRGSWST
                     GGGRRTWNQGGWQQTSSAQGGVIVRDHR"
     gene            5360..5950
                     /locus_tag="VFV99_30590"
     CDS             5360..5950
                     /locus_tag="VFV99_30590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063781.1"
                     /translation="MKKLAILLVVAAACGKSDKKSSAPPSAPIDVAAVNALVPAELKD
                     KLVFEKRDITEERGKRSTVTYTMAAPKDWEQDMKMFASVKPKSEANLGFMTSLQVGSN
                     CDGTCEPKDWAKTSEKVNFAQFRDGGKIVKDDLQKTSHLMITEKGDSTYVVYAWWSDG
                     AKRYFSCTASLEQEVKAAADAFAKACQAVNMAGDLN"
     gene            complement(5947..6123)
                     /locus_tag="VFV99_30595"
     CDS             complement(5947..6123)
                     /locus_tag="VFV99_30595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063782.1"
                     /translation="MPTCRECNDEVDKLVSVKVAGKARKVCSDCADRLREEGEIAEAS
                     EGVIQGMMGYKGRR"
     gene            complement(6138..8339)
                     /locus_tag="VFV99_30600"
     CDS             complement(6138..8339)
                     /locus_tag="VFV99_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063783.1"
                     /translation="MTSSVDLGRCFSGVCLPAGCGNGEVEPEEKCDDGNTIGGDGCSA
                     DCVSREVCGDGYADKLGGEECDDANTRGRDGCTNHCTLERPAWHLHLASEPSPRVLVS
                     AAYDSLAGNFVAFGGASTANAQLDETWTLDENGWTLANFGAPLRRTAPALAYDPQRRR
                     VVMFGGSSSNLQSVLLNDTWEWNGATWDRRNSPSVPAGRSGTGLAWDGAQLILFGGSQ
                     VTNKLQDTWSWSGGAWTRLAPQHAPTARSGHGMVFDPKHDRIVLFGGDTATSAQNDTW
                     TWNGTDWTQLAPSTSPPLMRWLALAYDAKRGVVVLSGVDKNTNGNVVWELDGAQWVNK
                     PVTASPNITSDAWLGYDERIQRVVQFGGYKLGGPLTNEVWEWDGANWTLRPAPSLPPA
                     RHLCTLASDPLRGRVVLFGGQGSTMPLNDTWEWDGRRWQARLGASPTARSSAAIAFDG
                     AEVMLFGGVGSTLLGDTWHYNGARWMQVASTGPSARFAAGMAYDAKRARVVLFGGDNG
                     GATSGTNDTWVWSGTAWTQLTTTTTPPARKFTQLAYDTKRDVIVMFGGISAIGVPLTD
                     TWELDGTDWVEKTPPVHPEARQGFNLVYDRARERVMLFGGSLGDFSLWEWDGTEWTQP
                     ETTIVPGTSREACAAYDDARRAIIAFGGDDGSPTRQTATGSYRGDGEEVCRAGLDLDE
                     DGAAGCADDDCGTVCAPVCWDDPTCTTAPRCGDGTCSTLETAEAACTADCP"
BASE COUNT         1493 a         2858 c         2783 g         1368 t
ORIGIN      
        1 agcgagccgg tccttgagca tgatctcgac cgagctccag ccgatgatcg gcacgccgtc
       61 ctggatcacg acgagatcga tgtcgtgcgc gtacagcttc gcgagcgcgt cgaagctgcg
      121 cacctcgtag gcctggcgcc actgctcgac cgtcgcgcgc gcggcggcga cgacttcctt
      181 cggcgtcgct gggcgcgcag gcgtcgtgtc gccgccactc cgcgggcacc ctgccagcac
      241 gagcgtgacg acgaagatcg cgacgtgtct catgcgcgct acataacacg ctgatgtgcg
      301 ggagcgcccg cttggcgcgc ccccagatgg tgaccacgat ttagtcatgg cgctcgaaca
      361 tctagagaac gacgaacgtc aggcgacgac cacgattctg ctcacggcca tcatgtccgt
      421 gatcatcacg ggattgatcg tcgctgccgt gctttcgact cgcgcatgct cggtgtagtg
      481 tccgcggcat gaccgagatc gtttggctcc atgcgcgcga agttctggat tctcgtggca
      541 acccgaccgt cgaagccgag gtcggtctcg atggcggcgt gctcggccgc gcgatcgtcc
      601 cgtccggtgc atcgaccggc gagcacgaag cactggagct ccgcgacggc gatgccaagc
      661 gctatctcgg caaaggcgtc accaaggccg tcgatcacgt ccgcgacgtt atcgcgccga
      721 cgctgatcgg catggacgcc agcgaccaag ccgcggtcga caaggcgctg atccagctcg
      781 acggcacgcc gatgaaatcg aatctcggcg cgaacgcgat cctctcggtg tcgatggcgg
      841 tcgcgcgcgc ggcatcgatg gcgcacgagc tgccgctgta tcgctacctc ggtggcgtcg
      901 gcgccgtgac cctgccggtg ccgctgatga acatcatcaa cggcggcgcg cacgccgata
      961 acaacctcga catccaggag ttcatgatcg cgccggccgg cttcgaccgg ttcgaagacg
     1021 cgctgcgcgc tggcgtcgag gtgttccaca atctgaagaa gctgctgtcg tcgcgcggca
     1081 aggcgacgaa cgtcggtgac gagggcggct tcgcgccgag cctcgattcg ggcgacgaag
     1141 cgctgcagct cgtgctcgag gcgatcgaca aggcgaacta cgagcctggc aagcaaatct
     1201 tcctcgcgct cgacgtcgcc gcgagcgagc tgttcgacaa gaagaccggc atgtacacgt
     1261 gggaaggcaa gcagcgcaaa gcggccgacc tcatcaacat ctacgccggc tgggcggaga
     1321 agtacccgct cgtctccgtc gaggacggtc tcgccgagga cgactgggac ggctggaagg
     1381 acctgaccga tcgcctcgac ggcaaggtcc agctcgtcgg cgatgacctg ttcgtcaccc
     1441 agaccgcgcg cctcaagcgc ggcatcgaca acggcatcgc gaactcgatc ctcgtcaagg
     1501 tgaaccagat cggctcgctc accgagacgc tcgacgccgt gcgcaccgca cagcacgccg
     1561 gctacacggc gatcatctcg caccgctcgg gcgagagcga ggacagcttc atcgccgacc
     1621 tcgcagtcgc gacgaacgcg ggccagatca agaccggctc ggcgtcgcgc agcgatcgca
     1681 tcgcgaagta caaccagctg ctccgcatct gcgatcagct cggtgacgaa gcgcggttcg
     1741 cgggcgccgg cgtgttcaag cgctgatgcc accaagcggt tactcgttca agagcaacct
     1801 gacgttcgcg cagatccgcg cgcgcctcgc cgagctcggc cacggtacgt gggaagagcg
     1861 cgaaagcgac atctacggct cgtacattcg gggtcaggca ttcggcgtgt ggatgcggat
     1921 ctacgatggt gtcggctgca catctgagct cggcacgtac gacccgaagg gcttcatgct
     1981 gctcgactat gcgcgccggg cactcgctga catcagcgcg gcccacgaca aacgtgtccg
     2041 cgaggagctg ttgcccgcgc tcgatgtcac ggagtggcac gaggacgagg ggaacgactg
     2101 agccgctgcg cgatccgttc cgcgaggcgc tcgagctcgt cgggaagcag cagcaccggc
     2161 cacatcgcga gcatgccgta cgggaacgac ccgaggcgca ggccgactgc gatgccgacc
     2221 tcgaacagca cgaacagaga aaaccagatc cagcgcagcc gcgcgcgatt cgcgaaccgg
     2281 cgcaggcggc caggtcgctc ggccgtcgcc gcgaagtaga gccagagcaa gtagaacagc
     2341 gccgtcagct cgaacgtgat ggtcagtgcc gtcgcgacgc gggtcagcgg atagatcgtg
     2401 ctgatgaagt ccgtcgacag ccggccgttg tgcggatcca tgagcgcgtt accgagcgcg
     2461 gtgaagccgc cgagcggatc ccaggcatgg ctcgacttgt tctggccacc cgagaaatag
     2521 acccagagca gctggaacaa gatcagataa cgcggccacg ccggcacgag cgcggacatc
     2581 ggccgaccga gcttgcgcaa cagccacgca tcgacggacc accgcgcgtt gcagcgcgac
     2641 agcgcgagca cgagcagcac gatgcggaac agctgatcga cgccactctc gctgtcgggc
     2701 gcgagcgccg cctgctggat cgatacggcg acgaatccga cgcatgcgat cggagtcgcg
     2761 atgccgaaca agaggcacgc gatcgcagcc atcgcgatcg cccacaagcc gaacgcgctg
     2821 aggccgagcg tatcggccac gccggtgaag cccgtcgcgt agccgtcggg gaaccgcgac
     2881 cacaacggat cgatgaggcc tgcgtgcgcg atccacgcgt aatcgacgag cagcacgagc
     2941 gcaatgccga tgcgcacgag cgcgagtgcg gtcgccggct cgcgccgatc catcagcgca
     3001 acccacgctc gccatgcgcg tgcgatgcgg ttcatggcgg accgttccgc agccggacgt
     3061 gctgcccgat gaactggcca ctcggcacca cgccgtcact cgtgatgtgc actttctcga
     3121 gccgcatgcg cgcggcgacg agctcgggat gccgatcgag cacgcgctgc gtgaggtagc
     3181 gcgcgagcac cgcgtactgg cccggcaggc gatcggttgg atcgtaacta ccgcgcatgc
     3241 gcgccgtgtc gatgaatgcc gcgtcgtcgg tgtgctcggg gtccgaggcg cgaaacagca
     3301 gctgccagcg gccttgcatg tcctcgcctt cgacccacag ccggtagcgc tccgtgctcg
     3361 gcgactgata gagcgaccag cgctgaccga cttgcagcgt tcgcccgatc cacgcgaccg
     3421 gtttcagcac gacatgctgg acgtcgcgaa tcggctcgac gaagcccttc tcccagtcag
     3481 gcgtctggtc ggctggcggc agcgggcagc catcgacgag cgcgaagaag atcgcaagca
     3541 ggatcaggcc tgcacggatc tcgggccagc gcttcacgcg ctcagcatat cgcgctcaca
     3601 cgaggcgcgc gggcggcaac ccggtatcgc gtgcatgaag gcgccgcagc ttgaacgacg
     3661 ccaccgagta catcgagatg ccgctccaca ggcagcgcgc accccacacg aatgcaaacg
     3721 gtgcgtagat cgtcgccgcg agcagaccga cgtcgttcgt cattgcaccg agccacatcg
     3781 ccggcgcgag tgagaccacg agcagcgcac cggccgcgat cgaccgccgc gcctggctgc
     3841 gcaggctcac cagcatcagc cgctcttcgg ccagatcggc gctcacccgt cgattgtact
     3901 gctaccgcgc cggatcgaaa cgcgtgactt ggcggaggaa cgacgcgtcg aggtactcgc
     3961 gcttgatcac ctcggccatc tcctcggcag tcgcgaaccg cgcgtagtcg acgcggatca
     4021 ccggaatgac cttcgagatg ctcgcgatga attcctggta accctcgtag agcgcctgca
     4081 ggtagccgag ctcgatcttc gcttcgacgt cgcggctgcg catgcgaatg cgctcggccg
     4141 atgcctcggg cgacacgtcg aggaagatga tcacgctcgg cttgcacatg aagttgctca
     4201 tgttccggaa cagctcgacg tacgtgcggt agtcgcgctc gtccatcagg ccggtcttcg
     4261 cgagcatctt cgcgaagatc gagtcttcgt agatcgtgcg gtcctgtacc gcgctcttgc
     4321 cgcgccagat aatctcttgg tgctgctgga agcggcggtt caagagatag acctgcatcg
     4381 cgaacgagta gcgcgcggtg tcgcggtaga agtccgcgag gtactcgttg tcggcgaccg
     4441 gctcgtagta gcagtcgatg ccgagatgct tgccgagctc ggcggcgagc gtcgatttgc
     4501 ccgcgccgat catcccggcg atgccgatga agatgttgcc gaagacgtcc ggcgcctggc
     4561 ccgtggccct tgtccctacc tgtgtcgggc cggatccggt ggcaataggt ggcacagcgg
     4621 acggctcgcg ggtgatttcg gtctcggtca gcacgaacgg agcctgccaa acgcctatga
     4681 catcgtggca agctcgacga atgcggaagg tcgcgatcgg tctggttctc gctacgttgg
     4741 tgtcgggttg cgtcgtgcga acgcacggcc gggtgaatcc gctcgcgagc atgctggtca
     4801 ccggcatcgc ggtcgccgcg gtcgctagca ccgtgcaggt gtcgaacccg ccgccgccca
     4861 ccgtcgaggt cggctactac ggctacgcgc gtcccggcta cgtctgggtc aacggctatc
     4921 accgctggaa cggcaacgcg tgggtttgga cgaacggcta ctaccaggcc gagcgcgccg
     4981 gctactactg ggtgcagccg agctgggagc tgcgtggcga tcaatacgtg tgggtcgatg
     5041 gctactgggc cgagccgcgc gccggctacg tctatgtcga cggctattac gactatcgcg
     5101 gcagcggcta ctactggacg cccggccgct gggaagcgga tcgccccggc tacacctatg
     5161 tccgcggcag ctggtcgacc ggcggcggcc ggcgcacctg gaaccagggc ggctggcagc
     5221 agacgtcgtc ggcacagggc ggcgtgatcg ttcgcgacca tcgctagcgc caggtttggc
     5281 agcactgcca gtgttggcag cgaggacgac gcgcgaatgc ctagttgcgc gggcacgaga
     5341 tgtggaataa cggccaggca tgaagaaact ggcgatcctc ctcgttgtcg ccgccgcgtg
     5401 cggcaagtct gacaagaagt cctctgcccc gccgtcggct ccgatcgacg tcgcggccgt
     5461 caacgcgctc gttcccgccg agctcaaaga caagctcgtg ttcgagaagc gcgacatcac
     5521 cgaagagcgc ggcaagcgtt cgacggtgac gtacacgatg gccgcgccga aggattggga
     5581 gcaggacatg aagatgttcg cgagcgtcaa gccgaagagc gaagcgaacc tcggcttcat
     5641 gacgtcgctg caagtcggca gcaactgcga tggcacgtgc gagccgaagg actgggcgaa
     5701 gacgtcggag aaggtgaact tcgcgcagtt ccgcgacggc ggaaagatcg tgaaggacga
     5761 tctccagaag acgagccacc tcatgatcac cgagaagggc gactcgacgt acgtcgtgta
     5821 cgcgtggtgg tcggacggcg cgaagcggta cttctcgtgt accgcgtcgc tcgagcaaga
     5881 agtgaaggcc gccgccgacg cgttcgcgaa agcgtgtcag gcggtcaaca tggccggcga
     5941 cttgaactaa cgccggccct tgtagcccat cattccttgg atcacgccct cgctcgcttc
     6001 ggcgatctcg ccttcttcgc gtagccgatc ggcgcaatcc gagcagacct tgcgggcctt
     6061 gcccgcgacc ttcacgctca ccagcttgtc gacttcgtca ttgcactcgc ggcaggtcgg
     6121 catccgtcga tcttagatca cgggcagtcc gccgtgcacg ccgcttcggc tgtctcgagt
     6181 gtgctgcacg taccgtcgcc gcagcgcggt gcggtcgtgc acgtcggatc gtcccagcac
     6241 accggcgcac acacggtgcc gcagtcgtca tcggcgcagc cggcggcgcc atcctcgtcg
     6301 agatcgaggc ccgcgcggca gacctcttcg ccgtcgccgc ggtagctgcc ggtcgcggtc
     6361 tggcgcgtcg ggctgccgtc gtcgccgccg aacgcgatga tggcccggcg cgcgtcgtcg
     6421 tacgccgcgc acgcttcgcg cgacgtcccc ggcacgatcg tcgtctcggg ctgtgtccat
     6481 tcggtgccgt cccactccca gagtgagaag tcgccgagcg agccaccgaa caacatcaca
     6541 cgctcgcgtg cgcggtcgta gacgagattg aatccctggc gtgcctcggg atgcacgggt
     6601 ggtgtcttct cgacccagtc cgtgccgtcg agctcccacg tatcggtgag cggcaccccg
     6661 atcgctgaga tgccgccaaa catcacgatg acatcgcgct tcgtgtcgta cgcgagttgc
     6721 gtgaacttgc gtgcgggtgg cgtggtggtt gtcgtgagct gtgtccatgc cgtgccgctc
     6781 cacacccagg tatcgttggt tcccgacgtg gcgccgccgt tgtcaccgcc gaacaacacg
     6841 acacgtgcgc gcttcgcgtc gtacgccatg ccggcagcga agcgcgccga gggtcccgtg
     6901 ctcgcgacct gcatccaacg tgcaccgttg tagtgccagg tatcgccgag cagcgtcgaa
     6961 cccacgccgc cgaacagcat gacttccgcg ccatcgaatg cgatcgccgc cgagctcctc
     7021 gccgtcggcg acgcaccgag cctggcttgc cagcggcggc catcccactc ccaggtgtcg
     7081 ttgagtggca tggtactgcc ctgcccgcca aacagcacga cacggccgcg cagcggatcg
     7141 ctcgcgagcg tacagagatg acgggccggc ggcagcgacg gcgctgggcg cagcgtccag
     7201 ttggcgccat cccactccca cacctcgttc gtcagcggtc ccccgagctt gtagccacca
     7261 aactggacga cgcgctgaat gcgctcgtcg tatccgagcc acgcatcgct cgtgatattc
     7321 ggcgaggcgg tgacgggctt gtttacccac tgcgcgccgt cgagctccca cacgacgttg
     7381 ccgttggtgt tcttgtccac gcccgacagc acgaccacac cacgcttcgc gtcgtacgcg
     7441 agcgcgagcc agcgcatcag tggtggcgac gtactcggcg cgagctgcgt ccagtccgtg
     7501 ccattccatg tccacgtgtc gttctgcgcg ctcgtcgctg tgtcgccgcc gaacagcacg
     7561 atgcggtcgt gcttcggatc gaacaccatg ccgtggccgc tgcgcgcagt tggcgcgtgc
     7621 tgtggtgcga gccgcgtcca tgcgccgccg ctccacgacc aggtgtcttg caacttgttc
     7681 gtgacctgcg agccaccgaa caggatcagc tgcgcgccat cccacgcgag tccggtgcca
     7741 gaccgccccg ccggcaccga cggtgagttg cgacgatccc acgtcgcgcc gttccattcc
     7801 caggtgtcgt tgagcagaac gctctgcagg ttgctcgacg aaccgccgaa catcacgacg
     7861 cggcggcgct gcggatcgta cgcgagcgcc ggtgcggtcc ggcggagcgg cgcgccgaag
     7921 ttcgccagcg tccacccgtt ctcgtcgagc gtccaggtct cgtcgagctg cgcgttcgcg
     7981 gtgctcgcgc caccaaacgc gacgaagttt ccggccagcg aatcgtaggc ggcactcacg
     8041 agcacgcgcg ggctcggttc actcgcgagg tggagatgcc acgccgggcg ctcgagcgtg
     8101 cagtgattcg tgcagccatc gcggccgcgc gtgttcgcgt cgtcgcactc ttcgccgccc
     8161 agcttgtcgg catagccatc gccgcacact tcgcgcgaca cacagtctgc ggagcagccg
     8221 tcgccgccga tggtgttgcc gtcgtcgcac ttctcttcgg gttcgacctc accgttgccg
     8281 cagccggcgg gcaagcacac acccgagaag cagcgcccca agtccaccga ggacgtcatg
     8341 cacgagtcga agtccatctg gcccacgcat gcggtcagct gctccgggtc gacgcagctg
     8401 tgatgcgcga gatcgcaggc ggtgccctgc gggcacagcc gatcgccgca ctctacgaga
     8461 tcgtcatcga tgcacccgac cagcgccagg gcgagcagcc ac
//
LOCUS       DASUFX010000755         9748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_816048,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000755 DASUFX010000000
VERSION     DASUFX010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816048"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFV99_30605"
     CDS             <1..630
                     /locus_tag="VFV99_30605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063784.1"
                     /translation="GSDGGGGGSDGGGGGSDGGGGTDAGMIAVDAMVPPGVITGGPCV
                     SGAMGATAYRIRWTNSGGTATVSYEVNGLPDTSPDHTGAYGYQIGFTPSYVDPFLAQG
                     GLQLDGSDFVDIDLSTVGVSTITNATLSIYGRSFNTTASGSFNWQTITGTGSTATNSV
                     SNVAPYAWYSGDMTTELPPNRDNLKIRIKAGPNSGSLVVNKIELCLQAT"
     gene            752..2845
                     /locus_tag="VFV99_30610"
     CDS             752..2845
                     /locus_tag="VFV99_30610"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5063785.1"
                     /translation="MRARPLCVVVMFVACSNSDGPYDYANSKGAALYQEQCQVCHGET
                     GEGGLGPQLRDSKKSVGDLEGIISERMPANAPGQCTGECASELAQFIREGLTSSALAC
                     NAVPPSARRLRLLTRREYRATVKDLFGTGAPAMSCARETDCAYRDTCSSGQCEPTACD
                     AQTFVFDPQGRTYSHVHVAGDFNQWASTIAAGGLPLAYDAATGLWAGTFTVGTGRHLY
                     KLVLDEQQWISDPRAPASESDGLGGQNSVMELACSASVDPIANLPVESRKAGFPFDTD
                     SAAALVTAAHVDAYLAAAEKLADFAAPVDVKTIGRRLFRRPLTADELTRYQALATMDP
                     KTALHAMLVSPEFLYRSELGEKHGDHYRLTPYEIATALSFMFVGTTPSDELLDAAEHG
                     ELDNAAGLEKWARALLADPRARSQVGELVLQWTGAQNVLTADKRADLFPDFTDATRAA
                     LVSETRSFAEHVVFDGSGTFGELMTADYTIVDPIAAKFYGFAGTGRVSYTDGRRAGLL
                     GQASVLATTAHSDQTSPVLRGLLVRRNFLCQELPPPPAFAGGLPAVDPNATTRDRFEM
                     HSKNGACAGCHHYIDSVGAGLERFDPVGRWRDTENGKPIAAGGNMRDVERLGTGTDAE
                     YETLPELAQTIATSTAAQSCFVRQYLRFSRGVRETLAERCARVKLEAKLASAGGDIRE
                     LMVQSVLSPDFVERR"
     gene            2842..4188
                     /locus_tag="VFV99_30615"
     CDS             2842..4188
                     /locus_tag="VFV99_30615"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5063786.1"
                     /translation="MITRRRLLQLGGAAAISAPLWRHLRAGNPGMQAKRFIVFYFPDG
                     IPGESQNGEPSMWTPSGGETSFTLPDLLSPLTPYRQSCVFFRGLSMGPADSGSHPGGA
                     KKLLTATDGGNGISIDQRLGSTVGANDPFRTLYLGAQANVNNASGDKHISYVAPGLTT
                     PPDDDPVAAFARVFDGLGTTTGGGGSDDPKRARRLAVLDNARAELEELSTKLGTTEKT
                     KLDLHLDAVREVERRIEALGDSGGGGTTCGQPAIDASGLSAATLYAPEKFPQVLRAQI
                     DLMVQAMACGLTRVGVIQGSHHTSELIMSRFANTEMYDPGFDMRSHQASHYGAAHDPA
                     KREYRAFAQQVKWWVAQYAYLLQQLAARPEGDGTMLDYSLVLLCSEVADGNTHLHDDM
                     PFVLAGGGGGTIRTGRFMDVGYRRHGDLFVAIARAMGDNVNSFGDASSGPLPGLLA"
     gene            4221..7841
                     /locus_tag="VFV99_30620"
     CDS             4221..7841
                     /locus_tag="VFV99_30620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5063787.1"
                     /translation="MDAGTLVSARFRIVRCIGSGATGDVYEAVDRDRGGRTAIKALRL
                     QHPLAINRFKHEFRVLQDLEHPNLVSLGELIEENGRLFFTMELVDGNHFLNYVRPNGT
                     GFDETRLRHAFAELGTALEALHARGVVHRDLKPSNVLATDDDRIVLLDFGLALDLEDQ
                     GPAWSGPNIVGTPHYMSPEQAAGQPAGPASDWYSAGVMLYEALTGGPPHEGSVMKILT
                     DKQSVEPTPPAALDPAIPADLSGLCSALLRFHPAQRPTSATGIRQVRREPRRRSSKRI
                     TGTPTQGPLFVGRQAELMQLRAILDSDVVRDGAPAVVLIRGESGVGKTTLARAFLRSI
                     ENDALVVRGRCFERESVPYKAFDGVVDGLTRVLGRMEPAVAAAMLPLHAAALADVFPD
                     LKRVKAFADAPTSNAAAHRDLRDHVFGALRELLVRLGRRHTLALAIDDLQWADEDSLA
                     LLEEIVRQPDAPPLMLLATLRPRNAPGVADIEARIRAACPQIRTIELGPLEPAASQEL
                     AERLCALHDVRDRLDAKQLADEAAGHPLFLDELVRHAASGGTGGGLRLDDALWERAAA
                     LDPKARVVLEVVSLAGEPLHHDTIVRAADLAPEQLVRVLSLLRVANLVRTTGAGADDS
                     VEPYHDRVRAAIVGHLDADRRRELHHALAIALEASQRRGHEPMLAFHWQEAGRSDLAA
                     GHAAAAAAAAVDTLAFERAVRFYRLALELGHPDRGELLTRLGEALGLDGRGPEAADAY
                     LAAADTTTAVEVAIERRRLAAEQLLISGHIDRGIELISTVLATLGMGLPATPQRALAS
                     LVLRRGLLRARGLRFRERQEGSVAASELTRVDLCWSVAGGLAMVDTIRGADFQTRHLL
                     LALRAGEPTRVARALATEGGFHSVGGVRSRRRAAKLFAEARALLARHPSEYVASMLTG
                     SECIHAYQAGEWRRALELARKAELEIRNQKATATWEHDTVDFFTLYSLFYLGEVRELA
                     ILAPALLRLATGRGDLFGATNLRVGLVNAAWLVPGDVEAAREHLADATRQWTARWFHL
                     QHYHHMFGEAHVALYAGEAEAGRRALLARWPALKASQILRISLIREEDRHLRARMAIA
                     AAFEGAPDRDALLREAAANAKDVSRVSEPAVAPLGELALAAVDHMRGDDERAVTRLRA
                     CVTAFERAEMGTYAAAARAALGRTLGGDEGAALVRAAEDAMHAQSVADPARWFAMLAP
                     GF"
     gene            complement(7838..9358)
                     /locus_tag="VFV99_30625"
     CDS             complement(7838..9358)
                     /locus_tag="VFV99_30625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063788.1"
                     /translation="MVEGITVVGVIEGDGPVLAATTLEIEEARHERKHGNRFVLRLDD
                     ERRVTVAATGKLAVVPAKPRRGTWRDVASDPRAALFADVAPGDHVDVKLLGWVLVPGQ
                     RVVVRGTVADEELSGGGPREAAVAQPSVIDSKRVAIVTTDDAAAISLLDEEEAHERRE
                     QQAKRARQAAAEAAQRGKTPRPLQRFIIELIVFAIILVVTGLACWRGVAAATAGRNTS
                     GVALLSITSFVIAYYAIAMLLVCLVVRPPFEAARCGEAEGTISPPWNNIPLLGGFFLL
                     GGAIMTASVLISVAFDGLEVLAFPVALCALGSFFFLWWGSAKHRAHRQLARLLGRARV
                     LPDPVEGQFGLFVGRVAPGSGWVIDRSITWQRHKYTNQSTHGPNAGQTSRWTDGTPSS
                     SSDPSFTVHCGSTKFVVETANAQWGAPLSYEPGTMISTRARIANRDEILVLARLRNEN
                     GTLVARSTGPESLVLFGAPQHARATLRRLAWRWLLPARALAALILASIAAIVWLAS"
BASE COUNT         1568 a         3314 c         3296 g         1570 t
ORIGIN      
        1 ggcagtgatg ggggcggtgg tggcagcgac ggtggaggcg gcggcagcga tggcggcggt
       61 ggaaccgacg caggcatgat cgcggtcgac gcgatggtgc cgcccggtgt gatcaccggt
      121 gggccgtgtg tctcgggcgc gatgggcgcg accgcgtatc gcatccggtg gacgaacagc
      181 ggcggcaccg caacggtctc ctacgaggtg aacggcttgc cggacacgtc gcccgatcac
      241 accggtgcgt atggctatca gatcggcttc acgccgtcgt acgtcgatcc gttcctcgcg
      301 caaggcggct tgcagctcga cggcagcgac ttcgtcgata tcgatctctc gacggtcggc
      361 gtctcgacga tcacgaacgc gacgctgtcg atctacggcc gcagcttcaa cacgaccgcg
      421 agcggcagct tcaactggca gacgatcacc gggaccggct cgaccgcgac gaactccgtc
      481 agcaacgtcg caccgtacgc gtggtactcg ggcgacatga cgaccgagct gccgccgaac
      541 cgcgacaact tgaagatccg gatcaaggcc ggtccaaact ccggctcgct cgtcgtgaac
      601 aagatcgagc tctgcctgca ggcgacgtaa gcgctgccaa caatgcgccg cactggcgcg
      661 gcgcgcgttg ccaaccacaa cgtggaccgt tcacggatcg tgtgccgtcg acttgttaca
      721 cgggcatgtg cgttgcagcc gccgtcgctc gatgagagca cggccgctct gcgtggtggt
      781 catgttcgtc gcttgctcga acagcgacgg cccctacgac tacgcgaaca gcaaaggcgc
      841 agctctgtac caggagcagt gccaggtctg ccatggcgag accggcgagg gcgggctcgg
      901 cccgcagctg cgcgactcga agaaatccgt cggtgacctc gaaggcatca tcagcgagcg
      961 gatgccggcg aacgcgcccg gccagtgcac cggcgagtgc gccagcgagc tcgcgcagtt
     1021 cattcgcgaa ggcctgacgt cgagtgcact cgcgtgtaac gcggtgccgc cgtcggcgcg
     1081 gcgattgcgg cttttgaccc gtcgcgagta ccgcgcgacc gtgaaggacc tgttcggcac
     1141 tggcgctccg gcgatgtcat gcgcacgcga gaccgactgc gcgtaccgcg atacgtgctc
     1201 gtcgggccag tgcgagccga ccgcgtgcga cgcgcagacg ttcgtattcg atccgcaggg
     1261 ccgcacgtat tcacacgtgc atgtcgccgg tgacttcaac cagtgggcct cgacgatcgc
     1321 tgcgggcgga ctgccgctcg cgtacgacgc cgcgaccggc ttgtgggccg gaacgttcac
     1381 ggttggcacc ggccgtcatc tctacaagct cgtgctcgac gagcaacaat ggatcagcga
     1441 tccgcgcgcg ccggcgagcg agagcgatgg cctcggcggc cagaactcgg tgatggagct
     1501 tgcatgctcg gcgagtgtcg atccgatcgc gaatctaccg gtcgagagcc gcaaggcagg
     1561 ctttccgttc gacaccgaca gcgcggcggc actcgtcacc gcggcacacg tcgatgcgta
     1621 cctggctgcc gccgagaagc tcgcggactt tgcagcgccg gtcgacgtca agacgatcgg
     1681 gcgccggcta ttccgccggc cgctcaccgc cgacgagctg acgcgctatc aggcgctcgc
     1741 gacgatggat ccgaagacgg cactgcacgc gatgttggtg tcgccagaat tcttgtatcg
     1801 ctccgagctc ggcgagaagc acggcgacca ctatcgactc acgccgtacg agatcgccac
     1861 ggcgctgagc ttcatgttcg tcggcacgac gccgagcgac gagctgctcg atgcggccga
     1921 gcacggcgag ctcgacaatg cagcgggtct cgagaagtgg gcgcgtgcac tgctcgcgga
     1981 tccgcgcgcg cgttcgcaag tcggcgagct ggtgctgcag tggacgggcg cacagaacgt
     2041 gctcaccgcc gacaagcgcg ccgatctgtt ccccgacttc accgatgcaa cgcgtgccgc
     2101 actcgtcagc gagacccgct cgttcgccga gcacgtcgtg ttcgacggca gcggcacgtt
     2161 cggcgagctg atgaccgccg actacacgat cgtcgatccg atcgcggcga agttctacgg
     2221 cttcgccggc accggtcgcg tcagctacac cgacggtcgc cgcgccggtc tactcggtca
     2281 ggcgtcggtg ctcgcgacga cggcgcactc ggatcaaacc tcgccggtgc tgcgcggctt
     2341 gctcgtacgg cgcaacttcc tgtgtcagga gctgccgcca ccgccggcgt tcgcgggtgg
     2401 gttgcctgcc gtcgatccga acgcgacgac gcgcgatcgc ttcgagatgc actcgaagaa
     2461 cggcgcgtgc gccggctgcc atcactacat cgattcggtt ggcgccgggc tcgagcgctt
     2521 cgatcccgtc ggacgctggc gtgacaccga gaacggcaag ccgatcgccg ccggcggcaa
     2581 catgcgcgac gtcgagcgcc tcggcaccgg caccgatgcg gaatacgaga cgctaccgga
     2641 gctcgctcag acgatcgcga ccagcacggc cgcgcagagc tgcttcgtgc gccagtacct
     2701 gcggttctcg cgcggcgtac gcgagacgct cgccgaacgc tgcgcgcgcg taaagctcga
     2761 agcgaagctc gcatccgcgg gcggcgacat tcgcgagctg atggtgcaga gcgttctctc
     2821 tcctgacttc gtggagcgcc gatgatcacg cgccgtcgcc ttcttcagct cggcggcgct
     2881 gccgcgatct cggcaccgct gtggcgccac ttgcgcgcgg gaaatcccgg catgcaggcg
     2941 aagcgcttca tcgtgttcta tttccccgac ggcattcccg gcgaatcgca gaacggcgag
     3001 ccgagcatgt ggacgccaag cggcggcgag acatcgttca cgctgccgga tctgctgtcg
     3061 ccgctcaccc cgtatcgcca gtcgtgcgtg ttcttccgtg gcctgtcgat gggacccgcc
     3121 gatagcggca gtcacccggg tggagcgaag aagctgctca ccgccaccga tggcggcaat
     3181 ggcatctcga tcgatcagcg gctcggctcg acggtcggcg cgaacgatcc gttccgcacg
     3241 ctctatctcg gcgcgcaagc gaacgtgaac aacgcctcgg gtgacaagca catctcgtac
     3301 gtggcgcccg ggctgacgac acctcccgac gacgatccgg tcgcggcgtt cgcgcgcgtg
     3361 ttcgacggtc tcggcacgac gacgggcggc ggcggttcgg acgaccccaa acgcgcacgc
     3421 cgcctcgccg tgctcgacaa cgcgcgcgcc gagctcgagg agctctcgac caagctcggc
     3481 acgaccgaga agacgaagct cgacctccac ctcgatgccg tgcgcgaagt cgagcgccgg
     3541 atcgaagcac tcggcgactc cggcggtggc ggcacgacgt gtggccagcc cgcgatcgac
     3601 gcatcggggc tctccgcggc gacgctgtac gcgcccgaga aattcccgca ggtgctgcgc
     3661 gcgcagatcg atctgatggt gcaagcgatg gcgtgcggac tcacgcgtgt cggcgtgatc
     3721 cagggctcgc atcacacgag cgagctgatc atgagtcggt tcgcgaacac cgagatgtat
     3781 gacccgggct tcgacatgcg cagccatcag gcctcgcact acggcgccgc ccacgatccg
     3841 gcgaagcgcg agtaccgtgc attcgcgcag caggtgaagt ggtgggtcgc gcagtacgcg
     3901 tatctcttgc agcagctcgc cgcgcggccc gagggcgacg gcacgatgct cgattattcg
     3961 ctcgtgctgc tgtgctccga agtcgccgac ggcaacacgc acctacacga cgacatgccg
     4021 ttcgtgctgg cgggtggcgg tggcggcacg atccgcaccg gccggttcat ggacgtcggc
     4081 tatcgccgcc acggcgacct gttcgtcgcg atcgcgcgcg cgatgggcga caacgtgaac
     4141 agcttcggtg acgcctccag cggtcccttg cccggcctgc tcgcctgatc ttcggcggcg
     4201 tcgtgggtta acatcgtcgg gtggatgcgg ggaccctggt ctcggcgcga tttcgcatcg
     4261 ttcgctgtat cgggagcggg gcgacgggag acgtctacga agcggtcgat cgcgatcgcg
     4321 gcggtcgcac ggcgatcaag gcgctgaggc tccagcaccc gctcgcgatc aaccggttca
     4381 agcacgagtt tcgcgtgctc caggacctcg agcatccgaa cctcgtatcg ctgggcgagc
     4441 tgatcgagga gaacggccgg ctgttcttca cgatggagct cgtcgacggc aaccactttc
     4501 tcaactacgt gcggcccaac ggcaccggct tcgacgagac ccggttgcgc catgcgttcg
     4561 ccgagctcgg cacagcgctc gaggcgctac acgcacgcgg ggtcgtgcat cgcgatctga
     4621 agccgtcgaa cgtgctcgcc accgacgacg atcgcatcgt cctgctcgac ttcggcctcg
     4681 cgctcgacct cgaggaccaa ggccctgcgt ggtctggacc gaacattgtt ggcacgccgc
     4741 actacatgtc tcccgagcag gccgcggggc agccggccgg cccggcgtcg gattggtact
     4801 ccgcgggcgt gatgctgtac gaggcgctga cgggcgggcc accgcacgag ggttccgtca
     4861 tgaagatcct caccgacaag cagtcggtcg agcccacgcc gccggccgcg ctcgatccgg
     4921 cgattccggc cgatctgtcg gggctgtgct cggcgctgct gcggtttcac cccgcgcaac
     4981 gtccgacgag cgcgaccggc atccgccagg tacggcgaga accgcgccgg cgatcatcga
     5041 agcggatcac cggcactccg acgcagggcc cgttgtttgt cgggcgacaa gcggagctga
     5101 tgcagctgcg ggcgatcctc gatagcgatg tcgtgcgcga cggtgcgcca gcggtcgtac
     5161 tgatccgtgg cgagtcaggc gtcggcaaga cgacactcgc gcgcgcgttt ctgcgctcga
     5221 tcgagaacga tgcgctcgtg gtgcgcgggc gttgcttcga gcgcgagtcg gtgccgtaca
     5281 aggcgttcga cggtgttgtc gacggactga cgcgcgtgct cggccgcatg gaacctgcgg
     5341 tcgcagcggc gatgctgccg ctacatgcag cggcgctcgc cgacgtgttc cccgatctca
     5401 agcgcgtcaa ggcattcgcc gatgcaccaa cgagcaacgc ggccgcccat cgcgatctgc
     5461 gcgatcacgt cttcggcgca ctccgcgagc tgctggttcg gctcggccgc cgtcacacgc
     5521 tcgcgctcgc gatcgatgat ctccagtggg ccgacgagga cagcctcgca ctgctcgaag
     5581 agatcgtgcg tcagcccgac gcgccaccac tgatgttgct cgcgacgctg cggccacgca
     5641 atgcgcccgg tgtcgccgac atcgaagcgc gcatccgcgc ggcatgcccg cagatccgaa
     5701 cgatcgagct cggtccgctc gagcctgcag catcgcagga gctcgccgaa cggctgtgtg
     5761 cgctccacga cgtgcgcgat cggctcgacg cgaagcagct ggccgatgag gcggcaggac
     5821 acccgttgtt cctcgacgag ctcgtccgtc acgcagccag tggcggaacc ggcggcggct
     5881 tacgtctcga cgacgcgctg tgggaacgtg cggctgcgct cgacccgaaa gcgcgcgtgg
     5941 tactcgaggt ggtctcgctc gcgggcgagc cgctgcacca cgacacgatc gtgcgcgcgg
     6001 ccgacctcgc acccgagcag ctcgtccgcg tgttgtcgct gctgcgagtt gccaacttgg
     6061 tccggacgac gggcgccggg gccgacgaca gcgtcgagcc ctaccacgac cgtgtgcgcg
     6121 cggcgatcgt cggccacctc gatgccgacc ggcgtcgcga gcttcatcac gcgcttgcca
     6181 tcgcgctcga ggcgtcgcaa cgccgtggtc acgaaccgat gcttgcgttt cactggcagg
     6241 aggccggccg ctccgatctt gctgcggggc atgctgcggc cgcggccgct gccgccgtgg
     6301 acacgctcgc gttcgagcgt gcggtgcgct tctaccggct cgcactcgag ctcggtcatc
     6361 ccgatcgcgg cgagctgctg acccggctcg gcgaggcgct cggcctcgac ggacgcggcc
     6421 ccgaggccgc cgacgcctat ctcgccgctg ccgatacaac caccgcggtc gaggtcgcga
     6481 tcgagcggcg ccggctcgcg gctgagcagc tgctgatcag cggccacatc gatcgcggta
     6541 tcgagctgat ctcgaccgtg ctcgcaacgc tcggcatggg gctgccggcg acaccgcagc
     6601 gtgcgctcgc ctcgctcgtg ctgcgacgag gcctgctccg cgcgcgaggt ctgcgctttc
     6661 gcgagcgcca ggaaggttcg gtggcagcgt ccgaattgac gcgcgtcgat ctgtgctggt
     6721 cggtggccgg tgggttggcg atggtcgaca cgatccgcgg cgccgacttc cagacccgtc
     6781 acctgctact cgcattgcgt gccggcgagc caacgcgggt cgcgcgcgcg ctcgcgaccg
     6841 agggcggatt tcattcggtc ggcggtgtgc ggtcgcggcg gcgtgctgcg aagctgtttg
     6901 ccgaggcgcg ggcgttgctc gcgcgccatc cgtccgagta cgtggcgtcg atgctcaccg
     6961 gttcggaatg cattcacgcg tatcaggccg gcgaatggcg tcgtgcacta gagctcgcac
     7021 gcaaagccga gctcgagatt cgcaaccaga aggcgaccgc aacatgggag cacgacacgg
     7081 tcgatttctt cacgctctat tcgctgttct atctcggtga ggtgcgcgag ctcgcgatac
     7141 ttgcgcccgc gttactccga ctcgcgaccg gacgtggcga tctgttcggg gcgacgaacc
     7201 tgcgcgtcgg cctggtcaac gccgcgtggc tcgtgcccgg tgacgtcgag gccgcgcgcg
     7261 agcacctcgc cgacgcgact cgccagtgga cggcgcggtg gtttcacctg cagcactatc
     7321 accacatgtt cggcgaggca cacgtcgcgc tttacgcggg tgaggccgag gcgggtcggc
     7381 gcgcattgct cgcgcggtgg ccggcgctga aagcgtcgca gatcctacgg atctcgttga
     7441 tccgcgaaga agatcgtcac ttgcgtgcgc ggatggcgat cgcggcggcg ttcgaggggg
     7501 cgccggaccg agacgcgcta ttgcgcgagg ccgcggcgaa cgcgaaggac gtttcccgcg
     7561 tcagcgagcc ggcggtcgct ccactcggcg agctcgcact cgccgcggtc gatcacatgc
     7621 gcggcgacga cgagcgtgcg gtgacacggc tgcgcgcatg cgtgaccgcg ttcgagcgtg
     7681 ccgagatggg gacctacgcc gcagccgcac gcgctgcgct cggccgcacg ctcggcggcg
     7741 atgaaggtgc cgccctggtc cgcgcggcgg aggatgcgat gcacgcgcag tccgttgccg
     7801 atcctgcccg gtggtttgcg atgctcgcgc cgggcttcta actggcgagc cacacgatcg
     7861 ccgcaatcga cgcgaggatc agcgcggcga gagcgcgcgc gggcagcagc cagcgccagg
     7921 cgagccggcg aagcgtcgcg cgtgcatgct gcggtgctcc gaacaacacg agcgactccg
     7981 gcccggtact gcgtgcgacc agcgtgccgt tctcgttccg caagcgagcg aggacgagga
     8041 tctcgtccct gtttgcgatc cttgcacgtg tgctgatcat ggtgccgggc tcgtagctca
     8101 gcggcgcccc ccactgtgca ttggctgtct cgacgacaaa cttcgtgctt ccacagtgca
     8161 ccgtgaacga cggatccgac gacgagcttg gcgtgccgtc tgtccagcgg ctggtttgtc
     8221 cagcgttcgg tccatgcgtg ctctggttcg tgtacttgtg tcgttgccac gtgatcgagc
     8281 ggtcgatcac ccagcccgag ccgggcgcga cgcgcccgac gaatagcccg aactgtcctt
     8341 cgaccggatc tggcaacacg cgcgcacgcc cgagcagtcg agcgagttgg cgatgcgcac
     8401 gatgcttcgc cgaaccccac cacaagaaga agaagctgcc gagcgcgcat agcgcgacgg
     8461 gaaacgcgag cacctcgagc ccgtcgaatg caacggagat cagaaccgac gccgtcatga
     8521 tcgccccgcc caacaggaag aagccgccca ggagtgggat gttgttccag ggtgggctga
     8581 tcgtgccctc cgcttcgccg cagcgtgcgg cttcgaatgg cgggcgcacg accagacaga
     8641 cgagcagcat cgcgatcgcg taatacgcga tcacgaatga cgtgatcgag agcagcgcga
     8701 cgccggatgt gtttcggcca gctgtggccg cggcgacacc tcgccagcac gcgagcccgg
     8761 tcacgacgag aatgatcgca aagacgatca gctcgatgat gaaccgctgc agtgggcgag
     8821 gtgtctttcc gcgttgtgcc gcttcggccg cggcctgtcg cgcacgcttc gcctgttgct
     8881 cgcggcgctc gtgcgcctcc tcctcgtcga gcagactgat cgctgctgcg tcgtcagtgg
     8941 tcacgatcgc gacgcgcttg ctgtcgatca ccgacggctg cgcgaccgcc gcttcgcggg
     9001 gcccgccgcc cgacagctcc tcgtcggcca cggtgccacg caccaccacg cgctggccgg
     9061 gcacgagcac ccagccgagc agcttgacgt cgacgtgatc gccgggcgcg acatcggcga
     9121 acagcgctgc gcgcggatca gacgcaacgt cacgccaggt tcctcggcgt ggcttcgccg
     9181 gaacgacagc gagcttgccc gtggctgcca ccgtcacgcg tcgctcgtca tcgagacgca
     9241 gcacgaaccg gttcccatgc ttgcgctcat gccgcgcctc ttcgatctcg agcgtggtcg
     9301 ccgcgaggac cggcccgtcg ccttcgatca cacccacgac ggtgatccct tccaccacct
     9361 tcgttatcgc acgaacgctt ggaggtagcc gcgtctcggg tacgagctgg ttagcgaggt
     9421 gcagcaactt gcggcaggga tctcctcccg tgtgcgccgc ccatcgcgtt cctgagcgac
     9481 tcccgcgttc acgcgacaaa ccaagcgtag cgaggccaac gttcgcggcg ctctgcgctc
     9541 acaacgtcga caaagtcgca gcaagcacac attgccgagc gtgcgcgatt ttgcgtaccg
     9601 tggagaaccg gatcgcagga tgggaatctt ctcaaagctc ttcaaaggcg agaacgggga
     9661 agccgagcaa gggaccgcag atccgggtgc acctgcggac gctgcagctt cgccgtcggc
     9721 acccgaaacc cacgcacctc agctcgag
//
LOCUS       DASUFX010000756        11074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2066961, whole genome shotgun sequence.
ACCESSION   DASUFX010000756 DASUFX010000000
VERSION     DASUFX010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066961"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..703
                     /locus_tag="VFV99_30630"
     CDS             74..703
                     /locus_tag="VFV99_30630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5063789.1"
                     /translation="MTRNPHLSLGSVTDPIKVALLTDDPLLRAGLSSLLAQVGSIHVV
                     DRDAAEVALWDAGVDAEKTLSRLGELRSLPMPTVAVVGDQAHVAPALAAGARGVVLRD
                     QVGPGIHAALAAVRSGLTVMDAELAQSLVPAQPVPHSNGKGRGELTEREKQVVQLLSE
                     GLSNKLIADRLGISDHTAKFHVNGVMMKLGASTRTEAVVEAMRRGLIRL"
     gene            complement(618..1079)
                     /locus_tag="VFV99_30635"
     CDS             complement(618..1079)
                     /locus_tag="VFV99_30635"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX5063790.1"
                     /translation="MSEESDHLRELYRRIYQVVRKIPKGSVATYGQVAELAGIPGGGR
                     VAGAAMKTSKPTDRLPWQRVIGKAGKLRGRIAIHDPVGAAVQRKLLEDEGVTVGDSGL
                     VALDVYGVLADRPRKRTAGSKRSVTGGSIRGASPRRPLRSGSKRRASSSRR"
     gene            1113..1787
                     /gene="moaD"
                     /locus_tag="VFV99_30640"
     CDS             1113..1787
                     /gene="moaD"
                     /locus_tag="VFV99_30640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5063791.1"
                     /translation="MQIQVLYFAVFRERLGTSDEVLEIAAGATVGDAITALAAKHPPI
                     AQLRGRFRVAVNQDFVDDTHVLHDRDEVALIPPVAGGASEAKCRHVELSDRTLSLDRC
                     INAVRGEGMGGIVTFTGMVRLHSRGATIDHLEYEAYAPMAIKVMTQLCDEIEAEIPGT
                     RIAVEHRVGKLAIGDIAVVIAAAAPHRAEAFTACRAMIDRLKDRVPIWKKEISVDGAE
                     WVGLGP"
     gene            complement(1798..2355)
                     /locus_tag="VFV99_30645"
     CDS             complement(1798..2355)
                     /locus_tag="VFV99_30645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063792.1"
                     /translation="MKYWLLVFAPLLVAVAALLVAMRTCRQEPPWIDTHATQIHVGQW
                     VADIPEHWRDIHELRTPIPGAGVPAPGARTLLYEAIEGGVLGQIDVFPMPPVHDEEGC
                     QNLGNVLKDEGGSQGLVVDSVRAATFVGDRGCVLAIRIQDQSGLLLIRSHGVFGVAVR
                     CLGREASLDGTGACDKLIYGLRLEF"
     gene            complement(2381..2848)
                     /locus_tag="VFV99_30650"
     CDS             complement(2381..2848)
                     /locus_tag="VFV99_30650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063793.1"
                     /translation="MRRVAVLLVLALVACKSDQKSGEKPHHHEDRSDPATAAPALSLA
                     VTIDGTPATWGADAFAKVTKSAATNNGGESRDVWSLRELAHTLVGPSARVVSVTGESG
                     AKPIDAAAWADSTRTPILQMTRRGTLKFRWANAAGAWDSEAEMKDVTKLEVLH"
     gene            complement(2845..4275)
                     /gene="gspN"
                     /locus_tag="VFV99_30655"
     CDS             complement(2845..4275)
                     /gene="gspN"
                     /locus_tag="VFV99_30655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HEX5063794.1"
                     /translation="MAINLPKLPFKLPHLPFDRLGPRTRKILRYVGFSLFAIIVFVFA
                     LQLTFPYNRVKDKVIEAMSEKYDVTISSVERGWMPGRVYFHGFSIRTRQTKPDETINQ
                     FYIDTLEVDVGILALLGGNISVDFDAKIGDRKIGFGHLSGNLQIASFGKGSITASVEG
                     DSLPGDALPMRAALGLPMTGKLDFDINTHLPVEKSKLGKTSINWQKATGSLTLKCPNS
                     CTFGDGKTKLKPLLKNTRNQVMVGEGIDFGKVTMDTLLAKATMKGGKLVLDKFDTTSK
                     DGVLKIEYMMTLEKNFDESMVAGCLRFKGSDDLLRREPKTHAAISTTGAELRSDGLFH
                     IRLTDRFKDMKRLNQECGPGTNTNNGEDFSRSNRPNLTVQPEEAAKPSVPIPPPTPTP
                     TIEPPPATPPITQPGSAAPGSGAGSAPPGAAPIPTGSPGETRGGGDNRGERGSDRGSD
                     RGDHPPPSGPVGEDQRPTPPAQPPVE"
     gene            complement(4275..4871)
                     /locus_tag="VFV99_30660"
     CDS             complement(4275..4871)
                     /locus_tag="VFV99_30660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063795.1"
                     /translation="MAINDTARDFWARISPRERRLVILAAIALPITLAIWLGLSIRDG
                     LVDMEARNQRTRKALNVLADLRAKGETHSKVDDVVSMMGTEPLSLPTYLDNAAKKANF
                     TLKSTSPHQPLPKNGFVTYSVSASLDDLTIEQLKTFLMEVEMGSKFVAVTHIDLRRDF
                     RDKAKLDVNLEVSTYAKEAPAKTEGGSDAEGSAKKGGV"
     gene            complement(4879..6528)
                     /gene="pilM"
                     /locus_tag="VFV99_30665"
     CDS             complement(4879..6528)
                     /gene="pilM"
                     /locus_tag="VFV99_30665"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX5063796.1"
                     /translation="MATRTFAVDLGAWSVKLAIASPGIRGATLLSVHERMVPLSDEPL
                     EDRMRQALVAMIEELRLREETGYIGVYGDQVFTQVLEFGFKALRRSELDKAVGGELEG
                     VVPVDLEDMVYCFEQIPSAPPTGPMPQDAMRGRVAPPAEGMRVLSYAMRRDRAEHIIA
                     MGQAAGFQPRGLLACGGAAAKLVSHVPSLMKARMDGPVAVIDIGHERTDLVVVHQNKA
                     VFSRSIGRAGKQVTEKIMEHWRMPWPTAEQAKHSDGFIASNAEPATSEAWDRISRVLA
                     PELTLFGRDIRQTIAACRARTGFSPIAALIVGGGSRLRGMGSFLTEQLGIPTWRPTQD
                     DILALAGPRISQEAALSLPIDSAAMTVGMAYDAAGGRPQFDLRSGALAAKVDLSFLRA
                     KAVPLGVAVLAVAAFAAGSAYADLYRLRKSEKTLSTRLANETAELYGTSKSAADVLGS
                     SAAAGGPASPMPRMSAYDIMLEINAHLPPKDKITLDIDKLDIDEQKVDMSGSAKTGEE
                     IDLLVSELKKIPCFKEIQRGATETGEGGVKKFKLTIPTTCM"
     gene            complement(6531..7847)
                     /locus_tag="VFV99_30670"
     CDS             complement(6531..7847)
                     /locus_tag="VFV99_30670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063797.1"
                     /translation="MSDTKPNKQTNKRTKERGVALIAVLIAIAITIVISNEFGTATTT
                     DISAAANYRDNMRAHFLARSAQNIAELVVRIQGRLDNIKELRGQVQVTDFADQIAGVF
                     CGSPEEVRDLVGFSTSDMQGLGGEIGTCGFNGAFATEDDKINLNCANNPAAAAYIQSA
                     LQGLVYFQAYDPVFEEADAEGWRRTREEQVNSIIDYIDRDSTKGVPRAGNNGGTEDYG
                     YESLRDKYRAKNNYIDTLGEVKLARGVDDRFWSLFGQAFTVYGGCKVNIAAISNPQLI
                     AAILYLSAKNPNDPVIMDPRKLFALAGIVAKAKQFGETFAKLDDFVTFVKDPMAAVTM
                     LAGSGGMQGSAATNALSTGLMPGEKIGLELDPKKLQTIATSGPRRTYKVEAYGEIPRK
                     QTDNEGRPVYPAVRSTITGVWDTKVFPQNARKQPVPKGAWVFSRED"
     gene            complement(7844..8530)
                     /locus_tag="VFV99_30675"
     CDS             complement(7844..8530)
                     /locus_tag="VFV99_30675"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEX5063798.1"
                     /translation="MTRRSSQAGLTLIEVMIASAVLVMMMTLAWRTIRNTSDVRQSSS
                     TYQERNHELRMALGRAVADFEAAYLSKNEDPNASHPRTLMVAKAGSKVAQISFSTLGH
                     RVLWSDANESEQTVVTYMAHDSKEHPGQTDWVRAERRRPSNEVPEDEPAEFDVLCRNV
                     EKVEIQFWNWKNLEWQDTWDTTQADGQRGWLPSRVKITLTVKGADDQDIKFTTQARIV
                     MQEPLLFSPT"
     gene            complement(8539..9612)
                     /locus_tag="VFV99_30680"
     CDS             complement(8539..9612)
                     /locus_tag="VFV99_30680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5063799.1"
                     /translation="MKRGVPNSSSDPYTCTCTSTARLTNVCSQNGSGQNASRENALGK
                     SASSKSASSKNTSTLFTMAQGRRRRGRQVREQRTRIVHEHVHVTRARVRQCGFTLLEV
                     MIALALLGLGMVVLIKSAAGNIFSTEESHMMGIATDLARGKMYDIEETLLKDGFTDSS
                     QSQEDEKCFEDEGWPTICYSYKVEEPKMPSFEDMQASAQKQAEDAAKLAGSGSAALLG
                     SDAAAGGFENSMLGGLLGMMGGGKQDITSAQGGALLQGQWSMFQEILKVSIRKVTLTV
                     KWKVLGRDRDMKVVAFFTDAAAMDKVLNGMGSVDINDNPTTGGTGGTGGTGGTGATGG
                     TGGTGGRGGTGGTGTFGGRGGGK"
     gene            complement(9609..10361)
                     /locus_tag="VFV99_30685"
     CDS             complement(9609..10361)
                     /locus_tag="VFV99_30685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5063800.1"
                     /translation="MRNPRGMTILELMIVLAIIGAGMFLARSGFRMLTKADLVENSTE
                     LAAVLKRTGQLAVEKGEMHRVIIDTDKQIYAVEMCQGERTISRNEAVRPDEEATKRAL
                     EKGKERLKDVPADGMTSDPEAATKQALSIAGHHIADKMCSPVDDPSSDAEGKGWVRTL
                     KANKGVKFKEIWVQHRDESTSKGQVAIYFFPTGSAEKAVVEITDGSETFSVLVSGLTG
                     RVELRDGVLSDVNDHMMRNALGDKDKPREDER"
     gene            complement(10385..10798)
                     /locus_tag="VFV99_30690"
     CDS             complement(10385..10798)
                     /locus_tag="VFV99_30690"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5063801.1"
                     /translation="MKRKLGKVVRNSQRGMTLLEIMIVLAILALVMGLVVGPRVMKLF
                     GESKVDIAKLTVRKFADEAFPQWSRSHPDKGCPDKLEDLSEYMDKKDTKDPWGNQYKL
                     FCPPNLPAGAKGIAISSPGEDGKEGTGDDIKSWDL"
     gene            complement(10813..>11074)
                     /locus_tag="VFV99_30695"
     CDS             complement(10813..>11074)
                     /locus_tag="VFV99_30695"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5063802.1"
                     /translation="ALTLKKSGQFPATMTHMVAVGERAGALEDMLERVAATYEAEVDT
                     KLGRFTALLEPLMLVVMGGVVAFIVFSILQPIMDLGQLSGPK"
BASE COUNT         1738 a         3668 c         3530 g         2138 t
ORIGIN      
        1 gctcgtcacg agatcgacct agctaaccag gcagggttga cccggacgta ctgcgtggcc
       61 atgttgcgtc actatgaccc gcaatcctca cctctcactc ggctcggtaa cggacccgat
      121 caaggtcgcg ctgctgaccg atgaccctct gctccgagct gggctctctt ccctgctcgc
      181 gcaagtggga tcgatccatg tcgtggatcg tgacgcggct gaagtcgcgc tgtgggatgc
      241 cggggtcgac gcagagaaaa ctctgagccg ccttggcgag ctacgcagcc ttccgatgcc
      301 gaccgtcgcg gtcgttggtg atcaggccca tgttgctccg gcgcttgccg ctggtgcacg
      361 tggcgtcgtg cttcgcgatc aggtggggcc tggcattcat gccgcgctcg ccgctgtccg
      421 cagcggcctg accgtgatgg acgccgagct cgcgcagagt ctcgtgcccg cgcaacccgt
      481 gccgcattcg aacggcaagg gtcgcggcga gctcaccgag cgcgagaagc aagtcgtgca
      541 gctcctctcc gagggtctgt cgaacaagct gatcgcggac cgactcggca tcagcgatca
      601 cactgcgaag ttccacgtca acggcgtgat gatgaagctc ggcgcttcga cccggaccga
      661 agcggtcgtc gaggcgatgc gccgcggatt gatccgcctg taaccgagcg cttgctgccg
      721 gccgttcgct tacgtgggcg gtcggcgagc acgccgtaga cgtcgagtgc gacgaggccg
      781 ctgtcaccca cggtgacgcc ttcgtcctcc agcagcttgc gctgcaccgc tgcgccgact
      841 ggatcgtgaa tcgcgatccg gccgcgcagc tttcccgctt tgccgatcac acgctgccac
      901 ggcagccgat cggtcggctt gctcgtcttc atcgccgcgc cggcaacgcg cccgccgcct
      961 ggaatgccgg cgagctcggc aacctgaccg taggtcgcga cgcttccctt cgggatcttg
     1021 cgcacgacct gatagatgcg gcggtacagc tcgcgcaggt ggtcggactc ttcactcatc
     1081 gcgaggtcga gagtctatag tgcaagcgcg cgatgcagat ccaggttttg tacttcgcgg
     1141 tgtttcgcga acggcttggc acctcggacg aggtcctcga gatcgccgcc ggcgcgaccg
     1201 tcggtgacgc gatcaccgcg cttgccgcaa agcacccgcc gatcgcgcag ctgcgcggcc
     1261 gctttcgcgt cgcggtgaac caggattttg tcgacgacac gcatgttctg cacgaccgcg
     1321 acgaggtcgc gctgattcct cccgtggcgg gcggtgcgag cgaagcgaag tgtcggcacg
     1381 tcgagctcag cgatcgcacg ctgtcgctcg accgctgcat caacgcggtg cgcggcgaag
     1441 gcatgggcgg catcgtcacg ttcaccggca tggtgcgcct gcacagccgc ggcgcgacga
     1501 tcgatcacct cgagtacgag gcgtacgcgc cgatggcgat caaggtgatg acccagctgt
     1561 gcgacgagat cgaagccgag attccgggca cgcgcatcgc ggtcgagcac cgcgtcggca
     1621 agctcgcgat tggcgatatc gcggtcgtga tcgcggcggc tgccccgcat cgcgcggaag
     1681 cgttcaccgc gtgtcgcgcg atgatcgatc ggctgaaaga tcgcgtgccg atctggaaga
     1741 aggagatcag cgtcgacggc gcggagtggg tcggcctcgg accgtagcgc cagaatccta
     1801 gaactcgaga cgcaggccgt agatcaactt gtcgcaggcg cctgtaccgt cgaggcttgc
     1861 ctctctgcca agacaccgca ctgcaacacc gaacacaccg tgcgagcgga tcaacagcag
     1921 tccgctctgg tcttggatcc tgatcgcgag cacgcaaccg cgatcgccga caaacgtcgc
     1981 tgctcgcacc gagtcgacaa cgaggccctg gctgcctccc tcgtccttga gcacgttgcc
     2041 gagattttgg cagccctcct cgtcgtggac gggcggcatc gggaacacgt cgatctgacc
     2101 gagaacgccg ccctcgatcg cttcatagag caatgtccgc gcgccgggag ccggcacacc
     2161 ggcgccagga atcggcgtac gtagttcgtg gatgtcgcgc cagtgctccg ggatatcagc
     2221 tacccactgc ccgacatgga tctgcgtcgc atgcgtgtcg atccatggcg gctcttgccg
     2281 gcatgtccgc atcgcaacga gcagcgccgc gaccgcgacg agcaacggcg caaacacgag
     2341 cagccagtac ttcacgcccc gagcgtaacc cgccgctacg ctagtggaga acttcgagtt
     2401 tcgtcacgtc cttcatctcg gcttcgctgt cccaggcgcc ggcggcgttc gcccatcgga
     2461 acttcagcgt gccgcgccgg gtcatttgaa gaatcggcgt acgcgtgctg tcggcccagg
     2521 ccgccgcatc gatcggcttc gcgccgctct cgccggtgac cgagacgacg cgcgcgctcg
     2581 gaccgacgag cgtgtgcgcg agctcgcgta gcgaccacac gtcgcgcgac tcgccgccgt
     2641 tgttggtggc ggcactcttc gtgaccttcg caaatgcgtc ggcgccccac gtcgccggcg
     2701 tgccgtcgat cgtgaccgcg aggctcagtg cgggagccgc agtcgccggg tcgctgcgat
     2761 cttcgtgatg atgaggcttc tcgcccgact tctggtcgga cttacacgca accagcgcga
     2821 ggacgaggag aacggcgacg cgcctcattc aacgggcggt tgcgctggtg gtgtcggccg
     2881 ctggtcctca ccgacgggac ccgacggtgg aggatgatcg ccgcggtcac tgccgcgatc
     2941 gctgccgcgc tcgccgcgat tgtcaccgcc gccacgcgtc tcgccggggc tgccggtcgg
     3001 gattggcgca gcacctggcg gcgcagaacc cgccccgctg cccggcgcgg cggagcctgg
     3061 ttgcgtaatc ggcggagtcg ccggcggcgg ctcgatcgtc ggcgttggtg tcggcggtgg
     3121 gatcggaacc gacggcttcg cggcttcttc gggctgcacc gtcaggttgg gacggttgct
     3181 gcggctgaag tcttcgccgt tgttcgtgtt ggtgcccgga ccgcactcct ggttgaggcg
     3241 cttcatatcc ttgaagcgat cggtgaggcg gatgtggaac aggccgtcgc tgcgcagctc
     3301 ggcgcccgtc gtcgagatcg cggcgtgggt tttgggctcg cggcgcagca gatcgtccga
     3361 gcctttgaag cgcaggcagc ccgcgaccat cgactcgtcg aagttcttct cgagcgtcat
     3421 catgtactcg atcttgagga cgccatcctt cgaggtcgtg tcgaacttgt cgagcacgag
     3481 cttgccgccc ttcatcgtcg ccttcgcgag gagcgtgtcc atcgtgactt tgccgaagtc
     3541 gatgccctcg ccgaccatga cctgattgcg cgtgttcttg agcagtggct tgagcttcgt
     3601 cttgccgtcg ccgaacgtgc agctgttcgg gcacttgagc gtcaggctgc cggtggcctt
     3661 ctgccagttg atcgaggtct tgccgagctt cgatttctcg acgggcaagt gcgtgttgat
     3721 gtcgaagtcg agcttgcccg tcattggcaa gccgagtgcc gcgcgcatcg gcaacgcgtc
     3781 gccggggagg ctgtcgccct cgacgctggc ggtgatgctg cccttgccga agctcgcgat
     3841 ctggaggttg ccgctcaggt ggccgaagcc gatcttgcgg tcgccgatct tcgcgtcgaa
     3901 gtcgacggag atattgccgc cgagcagcgc gaggatgccg acatcgacct cgagcgtatc
     3961 gatgtagaac tggttgatcg tttcgtcggg cttcgtctgg cgcgtgcgga tcgagaagcc
     4021 gtggaagtaa acgcggcccg gcatccagcc gcgctcgacg ctgctaatcg tgacgtcgta
     4081 cttctccgac atcgcctcga tcactttgtc tttgacgcgg ttgtacggga acgtcagctg
     4141 cagcgcgaac acgaacacga tgatcgcgaa caacgagaag ccgacgtagc gcaggatctt
     4201 gcgcgtacgc ggcccgagcc ggtcgaacgg caggtgcggc agcttgaacg gcagcttcgg
     4261 taaattgatc gccatcagac gccgcccttc ttcgccgagc cctcggcatc actgccgccc
     4321 tcggtcttcg cgggcgcttc cttcgcgtag gtcgagacct cgaggttgac gtcgagcttc
     4381 gccttgtcgc ggaagtcgcg gcgcagatcg atgtgggtga cggcgacgaa cttgctgccc
     4441 atctcgactt ccatgaggaa cgtcttcagc tgctcgatcg tgagatcgtc gagcgaggcc
     4501 gagaccgaat acgtgacgaa gccgttcttg ggcagcggct ggtgcggcga cgtcgacttg
     4561 agcgtgaagt tcgccttctt cgccgcgtta tcgaggtagg tcggcaagct cagcggctcc
     4621 gtgcccatca tcgagacgac atcatcgacc ttcgaatggg tctcgccctt ggcgcgcagg
     4681 tcagcgagca cgttgagtgc cttgcgcgtc ctctggttgc gcgcctccat gtcgacgagg
     4741 ccgtcgcgaa tcgagaggcc gagccagatc gcgagcgtga tcggcagagc gatcgctgcg
     4801 aggatgacga gccggcgctc gcgcggtgag atgcgggccc aaaaatcacg tgctgtatcg
     4861 ttgatcgcca tgactacctc acatgcacgt ggttggaatg gtgagcttga acttcttcac
     4921 gccgccctcg cccgtctcgg tcgcgccgcg ctggatttct ttgaagcacg gaatcttctt
     4981 gagctcggac acgagcaagt cgatctcctc gcccgtcttc gctgacccac tcatatcgac
     5041 cttctgctcg tcgatatcga gcttgtcgat gtcgagcgtg atcttgtcct tcggcggtag
     5101 gtgcgcgttg atctcgagca tgatgtcgta cgctgacatc ctcggcatcg gcgatgccgg
     5161 gccaccggct gcagcagacg agcccagcac gtcggctgcg ctcttggacg tgccgtacag
     5221 ctcggcggtc tcgttcgcga gccgcgtcga gagtgtcttc tccgatttgc gcagccggta
     5281 gagatcggcg tatgcgctgc cagctgcgaa tgcagcgact gcgagcaccg cgacgccgag
     5341 tggcaccgcc ttggcgcgca agaacgacag gtcgaccttc gccgcgagcg cgcccgagcg
     5401 cagatcgaac tgcgggcgac cgccggcagc gtcgtacgcc atgccaacgg tcatcgccgc
     5461 cgaatcgatc ggcaggctga gcgccgcttc ctgcgagatg cgcgggccgg cgagcgcgag
     5521 gatgtcgtcc tgcgtcgggc gccacgtcgg aatgccgagc tgctcggtga ggaacgagcc
     5581 catgccgcgc aaccgcgagc caccgccgac gatgagcgcc gcgatcggcg agaagccggt
     5641 gcgtgcccgg caggcggcga tcgtctgccg gatatcgcgg ccgaacagcg tcagctcggg
     5701 tgccagcacg cgcgagatgc gatcccacgc ttccgacgtc gccggctccg cattcgacgc
     5761 gatgaagccg tcgctgtgct tcgcctgttc ggcggtcggc cacggcatcc gccagtgctc
     5821 catgatcttc tcggtcacct gcttaccggc gcgaccgatg ctgcggctga acaccgcctt
     5881 gttctggtgc acgacgacga ggtcggtgcg ctcgtgaccg atgtcgatca cggcgaccgg
     5941 accatccatg cgcgccttca tcagcgacgg cacgtgcgag acgagcttcg ccgctgcacc
     6001 accgcatgcg agcagaccgc gcggctggaa gccggccgct tgccccatcg cgatgatgtg
     6061 ctccgcgcga tcgcggcgca tcgcgtagct gagcacgcgc ataccctcgg ccggcggcgc
     6121 gacgcggcca cgcatcgcat cctgcggcat cggaccggtc ggcggcgccg acggaatctg
     6181 ctcgaagcag tacaccatgt cctcgagatc gaccggcacc acgccttcga gctcaccacc
     6241 gacggccttg tcgagctcgc tgcggcgcag cgccttgaag ccgaactcga gcacctgcgt
     6301 gaacacctgg tcgccgtaga cgccgatgta gccggtctct tcgcgcagcc gaagctcttc
     6361 gatcattgcg acgagcgcct ggcgcatgcg gtcttcgagc ggttcgtcgc tcagcggcac
     6421 catgcgctcg tgcacgctga gtagcgtcgc gccgcgaata cctggactgg cgatcgcgag
     6481 cttcacgctc cacgcgccta gatcgactgc aaacgtccga gtcgccatgg tcagtcttcc
     6541 ctcgagaaca cccacgcgcc tttcggcacg ggctgctttc tcgcattttg tgggaacacc
     6601 ttcgtgtccc acactccggt gatcgtcgaa cgaacggccg gatacaccgg cctaccctcg
     6661 ttatcggtct gcttgcgcgg gatctcgccg tacgcctcga ctttgtacgt gcggcgcgga
     6721 ccgctcgtcg cgatcgtctg aagcttcttg ggatcgagct cgaggccgat cttctcgccg
     6781 ggcatgagac cggtcgacag cgcgttcgtc gccgcgctgc cctgcatgcc gccgctgccg
     6841 gcgagcatcg tgacggccgc cattggatcc ttcacgaacg tgacgaagtc atcgagcttc
     6901 gcgaacgtct cgccgaactg cttcgccttc gcgacgatac cggcgagcgc gaacagcttg
     6961 cgcggatcca tgatcaccgg atcgttcggg ttcttcgccg acagatacag gatcgcagcg
     7021 atcagctgcg ggttcgagat cgcggcgatg ttgaccttgc agccgccgta caccgtgaac
     7081 gcctgcccga acaacgacca gaaccgatcg tcgacgccac gcgcgagctt cacctcgccc
     7141 agcgtgtcga tgtagttgtt cttcgcgcgg tacttgtccc tgaggctctc gtagccgtag
     7201 tcctcggtgc cgccgttgtt gccggccctc ggcacgccct tcgtcgagtc acggtcgatg
     7261 taatcgatga tcgagttgac ctgctcttcg cgcgtgcgcc gccagccttc cgcgtcggcc
     7321 tcctcgaaca ccggatcgta ggcctggaag tacacgagtc cttgcagcgc tgactggata
     7381 taggcagcgg cagccgggtt gttcgcgcag ttgaggttga tcttgtcgtc ctcggtcgcg
     7441 aacgcgccat tgaaaccgca tgtaccgatt tcgccgccga ggccctgcat gtccgacgtc
     7501 gagaaaccga cgaggtcgcg gacctcctcg ggcgagccgc agaacacgcc ggcgatctga
     7561 tcggcgaagt cggtgacctg gacctgaccg cgcagctcct tgatgttatc gaggcgaccc
     7621 tgaatgcgca cgacgagctc ggcgatgttc tgcgcggagc gcgcgaggaa gtgcgcgcgc
     7681 atgttgtcgc ggtagttcgc agccgcgctg atgtcggtgg tcgtggcggt gccgaactcg
     7741 ttcgagatca cgatcgtgat ggcaatcgcg atcagcactg cgatcaatgc aacgccgcgc
     7801 tccttcgtgc gcttgttggt ctgcttattt ggcttggtgt cactcatgtc ggggagaaca
     7861 gcagcggctc ctgcatgacg atgcgggcct gcgtcgtgaa cttgatgtct tggtcgtcgg
     7921 cgcctttgac cgtcagcgtg atcttcacgc ggctcggcaa ccacccgcgc tgaccgtcgg
     7981 cctgcgtcgt gtcccaggtg tcctgccact cgaggttctt ccagttccag aactggatct
     8041 cgaccttctc gacgttgcgg cagagcacgt cgaactcggc gggctcgtct tcgggcacct
     8101 cgttcgacgg ccggcggcgc tccgcgcgga cccagtcggt ctggccggga tgctccttgc
     8161 tgtcgtgagc catgtacgtg accacggtct gctcggactc gttcgcgtcc gaccacagca
     8221 cgcgatggcc tagcgtcgag aacgagatct gcgccacctt cgagcccgcc ttcgcgacca
     8281 tcagcgtgcg cggatggctc gcgttggggt cctcgttctt cgacaggtac gcggcctcga
     8341 aatcggcgac cgcgcggccg agcgccatgc gcagctcgtg gttgcgctcc tggtacgtgc
     8401 tcgatgactg ccgcacgtcc gacgtgttgc ggatcgtgcg ccacgcgagc gtcatcatca
     8461 tgacgagcac cgcgctcgcg atcatcacct cgatcagcgt gaggccggct tgtgacgacc
     8521 gccgcgtcat ggcgagcctc atttgccacc gccgcggccg ccgaaggtgc ccgtaccgcc
     8581 ggtgccaccc cgaccaccag tgccaccggt accgccggtg gcgccggtgc cacccgtacc
     8641 gccggtgccg cccgtgccgc ccgtcgtcgg attgtcattg atgtccaccg agcccatgcc
     8701 gttgagcacc ttgtccatcg cggcggcgtc ggtgaagaac gcgacgacct tcatgtcgcg
     8761 atcgcggccg agcaccttcc acttcacggt cagcgtgacc ttgcgaatgc tgaccttcag
     8821 gatctcctgg aacatcgacc actggccctg cagcagcgcg ccgccttgcg ccgaggtgat
     8881 gtcctgcttg ccgccgccca tcatgccgag cagtccgccg agcatgctgt tctcgaatcc
     8941 accggcggcc gcatcggagc cgagcagtgc tgcggagcca gagcccgcga gcttcgcggc
     9001 atcctctgcc tgcttttgcg ccgaggcctg catgtcctcg aagctcggca tcttcggctc
     9061 ttcgaccttg tacgagtaac agatggtcgg ccagccctcg tcctcgaagc acttctcgtc
     9121 ttcttgcgat tggctgctgt cggtgaagcc gtccttcagc agcgtctctt cgatgtcgta
     9181 catcttgccg cgcgccagat cggtcgcgat gcccatcatg tgggactctt cagtgctgaa
     9241 gatgttgccg gccgcgctct tgatcagcac gaccatgccg agcccgagca gcgcgagcgc
     9301 gatcatcacc tcgaggagtg tgaatccgca ttgccgtaca cgtgcacgtg tcacgtgcac
     9361 gtgctcgtgc acgatccggg tgcgctgctc gcggacctga cgtccgcggc ggcgtcgccc
     9421 ctgcgccatc gtgaaaagcg tcgacgtgtt cttgctcgac gcactcttgc tcgacgcact
     9481 cttgcccagc gcattttcgc gcgacgcatt ctggcccgag ccgttctggc tgcagacatt
     9541 cgtgagtcgc gccgtcgacg tgcacgtgca cgtgtacgga tcagacgagg aattcggtac
     9601 gccgcgcttc atcgctcgtc ctcgcgcggc ttgtccttgt caccgagcgc gttgcgcatc
     9661 atgtgatcgt tgacgtcgct caggacgcca tcgcgcagct cgacacgacc ggtgagaccc
     9721 gacacgagca cgctgaacgt ctcgctgccg tcggtgatct cgacgaccgc cttctccgcc
     9781 gagccggtcg ggaagaagta gatcgcgacc tggcccttcg acgtgctctc gtcgcgatgc
     9841 tggacccaga tctccttgaa cttgacgccc ttgttcgcct tcagcgtgcg gacccagcct
     9901 ttgccttcgg catccgagct cggatcatcg acgggcgagc acatcttgtc cgcgatgtga
     9961 tgacccgcga tcgacagcgc ctgcttcgtc gctgcttcgg ggtcactcgt catgccgtcg
    10021 gcaggaacgt ctttcagacg ctccttgccc ttctcgagtg cgcgcttggt cgcttcctcg
    10081 tcaggacgga cggcctcgtt gcgcgagatc gtccgttcgc cttggcacat ctcgacggcg
    10141 tagatctgct tgtcggtgtc gatgatgacc cggtgcatct cgcccttttc gacggcgagc
    10201 tgaccggttc gcttgagcac cgccgcgagc tcggtcgagt tctcgacgag gtcggccttc
    10261 gtcagcatgc gaaagcccga gcgagccaaa aacatccccg cgccgatgat cgccaagacg
    10321 atcatcagct cgagaatggt catgccgcgc gggttacgca tggtggcgtc tcaggtcgcg
    10381 ttgcttagag gtcccaggac ttgatgtcgt ccccggtgcc ttccttgccg tcctcgccgg
    10441 gcgacgagat cgcgatgccc ttggcgccgg cggggaggtt cggcgggcag aacagcttgt
    10501 actggttgcc ccacggatct ttcgtgtcct tcttgtccat gtactcgctg aggtcttcca
    10561 gcttgtcggg gcagcccttg tcaggatgcg accgcgacca ctgcgggaat gcctcgtcgg
    10621 caaacttgcg aaccgtgagc ttcgcaatgt cgaccttcga ttcaccgaag agcttcatga
    10681 cgcgcggacc gacaacgagg cccatgacga gcgccaagat ggcgagcacg atcatgatct
    10741 cgaggagcgt catgccgcgc tgcgagttcc taacgacctt tccgagtttc cgcttcatat
    10801 cttccttcac tttcacttcg ggcctgagag ctgaccaagg tccatgatgg gttgcaggat
    10861 cgaaaacacg atgaaggcga ccaccccgcc catgacgacg agcatcaggg gctcgagcag
    10921 cgcggtgaac cggccgagct tggtgtcgac ctcggcctcg taggtggcag ccacacgctc
    10981 gagcatgtcc tcgagcgcac cggcgcgctc gccgaccgcg accatgtggg tcatcgtggc
    11041 gggaaactgg ccggatttct tgagcgtcag ggcg
//
LOCUS       DASUFX010000757         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1789649, whole genome shotgun sequence.
ACCESSION   DASUFX010000757 DASUFX010000000
VERSION     DASUFX010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789649"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VFV99_30700"
     CDS             complement(<1..1044)
                     /locus_tag="VFV99_30700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063803.1"
                     /translation="MKRFVLCALVAACAAPAATPAPPVIAKRAPDVPMSEPSEYDIDR
                     VTRAAGEAIFTRTGLGDPYKTGIPYPIMLGLMRGYPKTFGADWAEASRKFGFVARAAD
                     PQSDDPDVRAGLPIGWHLTTDPITGVQLMVHNCALCHVERVKWGTGEATIVGLGNKRI
                     RVHAFDIAFATVAEQRGFTVDKLRRLAGEAARDNKIAWPDQYADAFVGAAFTGLRTRA
                     EERAELHTRVAHGLPGRVATVDAFIPALRKLAGKHIEYSPAVGWAKVPDVIGFRHRTT
                     LSWDGAGEGPMAVLLIEADLAEGARVEWFERHPFQGASLDAFLRTPSPRPTFPGKFDR
                     NLAERGKVLFEQHC"
     gene            1170..1955
                     /locus_tag="VFV99_30705"
     CDS             1170..1955
                     /locus_tag="VFV99_30705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063804.1"
                     /translation="MRVLLAFVALLAGCKKEDKAAPAPSSPEVAPQPVTVAVADAAPP
                     AEPARPAGDPARGTPCPTGSALREAVAKAFGIAKPEDVDTTCNEMRTPQPVWAIDANA
                     FTGDLFEGSLALLDTHDQHIITKLSTGDSDQGLAKATITVADLDGDGIDELMRSATVG
                     GHGFANETLHILIVRDNKLVEVGKLPVRSENTGAVAIGEVKSKEHYECQSTMKVIKGA
                     SGKQLIEIVGDKRKGKPPVEECPPVGRHVYAFDGKAVTETKAK"
     gene            complement(1956..>2385)
                     /locus_tag="VFV99_30710"
     CDS             complement(1956..>2385)
                     /locus_tag="VFV99_30710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063805.1"
                     /translation="NHLPLNKLPIANLLGIKKFLKGFFRTEDGRVMGYNSPVKNNALD
                     GRWHVAEKKFGYYEVLPVDPTARDNKYLHALLLDYGKGKNPPLEPSQVLRDYLVQVDP
                     NNPNLYLGKADAAIGPLRIPTTNFFILERFRVGLTDYVKR"
BASE COUNT          410 a          758 c          839 g          378 t
ORIGIN      
        1 gcagtgctgt tcgaacagca ccttgccgcg ctcggcgagg ttgcgatcga atttgccggg
       61 gaacgtcggc cgcggcgacg gcgtgcgcag gaatgcatcg agactcgcgc cctgaaacgg
      121 gtggcgctcg aaccactcga cgcgcgcgcc ctcggcgagg tccgcttcga tcaggaggac
      181 ggccattgga ccttcgccag cgccgtccca cgacagcgtc gtgcgatggc gaaagccgat
      241 cacgtcggga actttcgccc agcccacggc aggcgagtac tcgatgtgct tgccggcgag
      301 cttgcgcagc gcggggatga acgcgtcgac ggtcgcgacg cggccgggca ggccgtgggc
      361 gacgcgcgtg tggagctcgg cgcgttcctc agcacgcgtg cgaaggccgg tgaacgcggc
      421 gccgacgaac gcgtctgcgt attgatcggg ccatgcgatc ttgttgtcgc gtgccgcctc
      481 accggcgagc cggcgcagct tgtcgaccgt gaagccgcgc tgctcggcga ccgtcgcgaa
      541 cgcgatgtcg aacgcgtgga cgcggatgcg cttgttgccg agaccgacga tcgtcgcctc
      601 gccggttccc cacttcacgc gctcgacgtg gcacagcgcg cagttgtgga ccatgagctg
      661 cacgccggtg atcggatcgg tcgtcaggtg ccaaccgatc gggagcccgg cgcgcacatc
      721 cggatcgtcg ctctgcggat cggccgcgcg cgcgacgaag ccgaacttgc gcgatgcctc
      781 cgcccagtcg gcgccgaacg tcttcggata gccacgcatc agcccgagca tgatggggta
      841 cgggatcccc gtcttgtacg gatcgccgag cccggtgcgc gtgaagatcg cctcgccggc
      901 ggcgcgcgtg acgcgatcga tgtcgtactc ggagggctcg gacatcggca catcgggcgc
      961 gcgcttcgcg atcacgggcg gcgccggcgt ggcggcgggt gcggcgcagg cggccaccag
     1021 cgcacagaga acgaatcgct tcatagctcg tcggctccta ggtcgggcgt gccggttcat
     1081 ggctcggccg tcgtcgccgc aggatgcggt tgccacgacg agccggacga gcgccccgcg
     1141 cttcatgtcc gcatgttact cctggagtca tgagagtgtt gctcgcattc gtggcgctgc
     1201 tcgctgggtg caagaaggaa gacaaggcag caccagctcc gtcctcgccg gaagtggcgc
     1261 cgcagcctgt gacggtcgca gtcgccgacg cggcgccacc cgccgagccg gccaggcccg
     1321 ctggcgaccc ggcccggggc acgccgtgcc cgacggggag cgcgctccgc gaagccgtgg
     1381 cgaaggcatt cggcatcgcg aagcccgagg atgtcgacac cacgtgcaac gagatgagga
     1441 caccgcaacc ggtctgggcg atcgacgcga acgcgttcac cggcgatttg ttcgagggct
     1501 cgctcgcgct gctcgatacg cacgatcagc acatcatcac gaagctgagc accggcgatt
     1561 cagaccaggg cctcgcaaag gccacgatca ccgtcgccga tctcgatggc gacggcatcg
     1621 acgagctcat gcggtcggca accgtcggtg gccatggctt cgcgaacgag acgctgcata
     1681 tcctgatcgt gcgcgacaac aagctcgtcg aggtcggcaa gctgccggtg cgctccgaga
     1741 acaccggcgc ggtcgcaatc ggcgaggtga agtcgaagga gcactacgag tgccagtcga
     1801 cgatgaaggt gatcaagggc gccagtggca agcagctaat cgagatcgtc ggggacaagc
     1861 gaaagggcaa gccgccggtc gaggagtgcc cgccggtcgg ccgccacgtc tacgcgttcg
     1921 acggcaaagc cgtcaccgag acgaaagcga aatagttaac gcttcacgta gtcggtgagg
     1981 ccgacgcgaa agcgctcgag gatgaagaag ttcgtcgtcg gaatgcgcag cgggccgatc
     2041 gcggcgtctg ccttgccgag atacaggttc ggattgttcg gatcgacctg cacgagatag
     2101 tcgcgcagca cctgcgatgg ttcgagcggc gggttcttgc ccttgccgta gtcgagcagc
     2161 agcgcgtgca ggtacttgtt gtcgcgcgcg gtcggatcga ccggcaggac ttcgtagtag
     2221 ccgaacttct tctcggcaac gtgccagcgg ccgtcgaggg cgttgttctt gaccggcgag
     2281 ttgtagccca tcacgcggcc gtcctcggtg cggaagaagc ccttgaggaa cttcttgata
     2341 ccgaggaggt tcgcgatcgg caacttgttc agcggcaggt gattg
//
LOCUS       DASUFX010000758         7935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2067272, whole genome shotgun sequence.
ACCESSION   DASUFX010000758 DASUFX010000000
VERSION     DASUFX010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067272"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..2449)
                     /locus_tag="VFV99_30715"
     CDS             complement(206..2449)
                     /locus_tag="VFV99_30715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063806.1"
                     /translation="MQKRLLLATGVLLLGVQTAHAIPYETFIDIDDEADLQDLLAAGT
                     ITDDTYNELLDLLTRGVDLAKADRNELYTLPNLTYEDVDAIIAYRDLQKGRIADPAEL
                     VAAGALSEEKLLAISSFIVVRKPGEDPLTVHGWVRAMTRAEPHDFAPFQPTDPLVPPI
                     AIRARFTALRYLMAGFALTNTRLEVGDAVYDPNRGALLAEPRSNQVHLPKAYVKFENK
                     DVTAIAGTYRAGFGQRLVFDNSLYYTPNGLYLDDQLYYSADLASACQESAGELAVSPC
                     LGAAGSEYVSPDFAWRDGLLGIGVGAKHLEMTTGWLQAYAWASLNRKGIYQYELVDSG
                     KCTDPHDDDNPDCAAPTVYVKPDGDLLTPTPRHSFATLPNIFLEKVVGGNVGYFADRR
                     NSIGLTAYGAQETDLVDGIDLSVQEWSRMPYGGKFGATGANFSFGRDWLDLFGEAAFS
                     FDSIPKPAPALNSPANGGGGPAGLIRMTATRKKEELEIVARYFSTDFVNPFSRPISEP
                     DEFEGQRARDETGVRARYIRATKEFQLRALVDFWVNQSAPNNKFLGIEMPPPKLDTYV
                     RAMVKTAPELWVGLWERYQDKDLTRGGHDQCFEISNDTDENGEPIPCGGRQLTTFLRA
                     QYRPDRKVQIDAQLGHYLLDDNTQMELENKFRQDISAWVIGYYRPNKDWRLRGRIRYL
                     DEAIHDNTYLERSVAGQVEASIRARERDLVRVRVDGKWWLDKRMSTTERAPNPELSLW
                     LFYEAKL"
     gene            complement(2480..2968)
                     /locus_tag="VFV99_30720"
     CDS             complement(2480..2968)
                     /locus_tag="VFV99_30720"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5063807.1"
                     /translation="MHVTRAALLAGFSLGVASGCGGSPCGDTEGKVTRVIDGDTIELE
                     SGVKIRYLMVDTPETTGSVDDCYGPNALQYNTDLVLNKTVQLSFDEQCTDRYGRTLAY
                     VTVDGQEVNSLLVRNGFACVLYIPPNGQSRVEEFNALQLDAKTARRGLWSACNPLPPA
                     CT"
     gene            complement(2959..4719)
                     /locus_tag="VFV99_30725"
     CDS             complement(2959..4719)
                     /locus_tag="VFV99_30725"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5063808.1"
                     /translation="MRFACLAAALVAAFAACGPDSSSGPCKDDLLAGDLVITEVFADY
                     AAPTGGTGTDDGKEWFEVYNNSDRPLSLEGLTVVHSRPDGSKAQSHTMTDVTIAPGQY
                     FTLGNSTSDLVPAYVDYGYSADLGDFFNSDGGKLALKCGDKEIDSAQYELVKSGHSRQ
                     LSNLGPPDYTANDDLANWCEGHDTEFEPNNFGTPGQDNDCAPVISGACSDGGSMRPVV
                     SPAPGELVITEVMPSPSKVSDTAGEWFEAKALAAFDLNGLGLDRAGDTSNPTTISSPT
                     CIHVDTGDYVVFAKSVDSSMNGGVTGAVGTFGFSMVAGSATSPGDVQVLSGSTVIDAI
                     SWTKSTTGKALQLDPDLTDATSNDNSSNFCDATATYGLGDFGTPGMVNGQCTMLPPPG
                     MCDDGGTLRAIVKPAANALVISEIMPNPKAEPGQEWFEVTNVGTASFDLNGLGVGRVS
                     SASMPNVINSAACKPVAPGAYALLAHSADPAMNGGLPTPDATFSVALVNGNGDIQVSD
                     PTTCTGTPVTCTTVYDAVVYTTANGWPSTATADGVSAQLKPTNLTTTDNDSFANFCPA
                     LTDYGTAMNKGTPKAANACM"
     gene            complement(4734..5849)
                     /locus_tag="VFV99_30730"
     CDS             complement(4734..5849)
                     /locus_tag="VFV99_30730"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5063809.1"
                     /translation="MKRLAVIAALALVGCGGHSDRYTRKAAQKSLLKLETPGLVIGEF
                     RVTKITDGDTIRVDGLDSSLRLLGVDTEETFKSEGDRRAFEGGWPQYMAMKRGNSPRP
                     VKFATPLGEDAKHFAEKFFSGSDKVRLERDHPAEIRDRYQRYLAYVFVNRNGTWVNYN
                     IEAVRAGMGPYFPKYGQSRRFHKEFVEAQEEARKAKRGIWADGVMAYPDYDERLAWWN
                     ARGDFVAQFRTEGEGKANYVDITHWDATKQLEDLVGKEVNVLGTVGDVRIGEKGPTRV
                     TLSRQQFSDFPLVFFDRDVFVQSNIAEQKGEFVWITGVPTIYENPHNHKKVVQIVIDR
                     ASQVRVSQVPPGKPPAPAKPPGPAPTPPAIPTSSNTR"
     gene            complement(5846..6403)
                     /locus_tag="VFV99_30735"
     CDS             complement(5846..6403)
                     /locus_tag="VFV99_30735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063810.1"
                     /translation="MRVIGLTVVLAVGCVREPAPAECPPIERGDLIVTELRGEQSPED
                     SLGIWVELFNASGATVDLEGTKIRFRKKDGSSEVPIIVRRSLTVPGGGYVTLGLSYDD
                     DTKPAAIDYGFAMDFHQGFLPAAAVDVEACGTLIDRAVYDVLPDTGTYSLGVTPDVDA
                     NDNPANWCVNPTPEGTPRQANPACP"
     gene            6462..7250
                     /locus_tag="VFV99_30740"
     CDS             6462..7250
                     /locus_tag="VFV99_30740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063811.1"
                     /translation="MQPPVGGGYPPSTPAAAPKPADPARSAAIMLVIAAVLILIGTVS
                     KNWVSGGRGSRDIHIGPMGGEACMSGACVDIPTRGIDGDIEAIMVLALISGFASAAAA
                     GTFGGMALAGKTDKIPLPPRLAQMAFGLAAFSMTFFVIRMISEKGELSWAGFPAIGGV
                     ILAGVGLKKLTPFLAAKPSVPPGAQQQFANQQPYGNQSQPMQPYGQQSQPMQPYANQS
                     QPMAAPQQPPPGAAPIHNCPRCGTQLHWVAQYQRWFCPREQQYV"
     gene            7247..7402
                     /locus_tag="VFV99_30745"
     CDS             7247..7402
                     /locus_tag="VFV99_30745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063812.1"
                     /translation="MSVDKLRTLEELLVWCRAHRADLVDVVVQDEFTHDVIVREADDT
                     YLCFDTT"
     gene            7552..>7935
                     /locus_tag="VFV99_30750"
     CDS             7552..>7935
                     /locus_tag="VFV99_30750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063813.1"
                     /translation="MTLRSFGLCGLLLAACSEGGSSEMHTDARPIPDAHDQIDGSSQV
                     PYRHAIALDGVDDFALVDQFATTSASFEARVTWDEKNIYVGYSGPDLMTSTSDANTKW
                     LFVYLDTKPGGEAQSELYNTQRATFP"
BASE COUNT         1308 a         2541 c         2657 g         1429 t
ORIGIN      
        1 actcgtccgc gctgacgtcc ttctgcttga gcgtgtctcg cacgccgagt ccgaagggca
       61 ctgccgtcac caaggacatc acgatcaccg acgtcggaat gcgcatcggg gtgacgctat
      121 caccggggcg ccgcgagtca acggaatgct tcatgcgtga tcgagccgcg gccgcggccc
      181 gagtcgcacg aaaaaacgcg ggtgtttaga gcttggcttc gtagaacaac caaagactta
      241 gctcgggatt gggcgctcgc tcggtcgtgc tcatccgctt gtccagccac cacttgccat
      301 ccacgcggac ccggacgagg tcgcgctcgc gcgctcggat cgatgcctcg acctgcccgg
      361 cgaccgaccg ctcgagatac gtgttgtcgt ggattgcctc gtcgaggtag cgaatgcggc
      421 cgcgcaggcg ccagtctttg ttcggccggt agtagccgat cacccacgcc gagatgtcct
      481 gccggaactt gttctcgagc tccatctgcg tgttgtcgtc gagcaggtag tggccgagct
      541 gcgcgtcgat ctggaccttg cggtcgggac ggtattgcgc gcgcaagaac gtcgtgagct
      601 ggcggccacc gcacggaatc ggctcgccgt tctcgtcggt gtcgttcgag atctcgaagc
      661 actggtcgtg accgccgcgc gtgaggtcct tgtcctggta gcgctcccac aggccgaccc
      721 acagctcggg cgccgtcttg accatcgcgc gcacgtaggt gtcgagcttc ggcggaggca
      781 tctcgatgcc caggaacttg ttgttcgggg ccgactgatt cacccagaag tcgaccagcg
      841 cgcgcagctg gaattccttc gtcgcgcgga tgtagcgggc gcgcacgccg gtctcgtcgc
      901 gggcgcgctg gccttcgaac tcgtcgggct ccgagatcgg ccgcgagaac gggttgacga
      961 agtccgtcga gaaataccgc gcgacgatct cgagctcttc tttcttgcgc gtcgcggtca
     1021 tgcggatcag gccggccgga ccgccgccgc cgttcgccgg cgagttgagc gcgggcgccg
     1081 gcttcggtat cgagtcgaag ctgaacgccg cttcaccgaa cagatcgagc cagtcacggc
     1141 cgaacgagaa gttcgcgccc gtcgcaccga acttgccgcc gtacggcatg cgcgaccatt
     1201 cctgtacgga caggtcgatg ccgtcgacca gatcggtttc ctgcgcgccg tatgcggtga
     1261 ggccgatcga gttgcggcga tcggcgaagt agccgacgtt gccgccgaca accttctcga
     1321 gaaagatgtt cggcagcgtt gcgaacgagt ggcgcggcgt cggcgtcagc agatcgccgt
     1381 cgggtttcac gtacacggtt ggcgccgcgc agtcggggtt gtcatcgtcg tgcgggtccg
     1441 tgcacttgcc ggaatcgaca agctcgtact gatagatgcc cttgcgattg agcgacgccc
     1501 acgcgtacgc ctgcagccag ccggtcgtca tctcgagatg cttcgcacca acgccgatgc
     1561 cgagcagacc atcgcgccac gcgaagtcgg gcgagacata ctcggagccg gcggcaccga
     1621 ggcacggcga caccgcgagc tcgccggccg actcctggca ggccgacgcg agatccgccg
     1681 agtagtagag ctggtcgtcg aggtagagac cgttcggcgt gtagtagagc gagttgtcga
     1741 acacgagccg ctggccgaag ccggcgcggt acgttcccgc gatcgcggtg acgtccttgt
     1801 tctcgaactt cacgtacgcc ttcggcaggt gcacctggtt cgagcgcggc tcggcgagta
     1861 gcgcgccgcg gttcggatcg tagaccgcgt cgccaacctc gaggcgcgtg ttcgtcagcg
     1921 cgaagccggc catcagatag cgcagggcgg tgaaccgcgc gcggatcgcg atcggcggca
     1981 cgagcgggtc ggtcggctgg aacggcgcga agtcgtgcgg ttctgcgcgg gtcatcgcgc
     2041 ggacccagcc gtgcacggtc agcgggtctt cgccgggctt gcgcacgacg atgaacgacg
     2101 agatcgcgag cagcttctcc tcggagagcg cgcctgcggc aacgagctcc gcggggtcgg
     2161 cgatcctgcc cttctgcaga tcgcgatacg cgatgatcgc gtcgacatcc tcgtaggtca
     2221 ggttcggcag cgtgtagagc tcgttgcgat cggctttcgc gaggtcgaca ccgcgcgtga
     2281 gcagatcgag gagctcgttg tacgtgtcat cggtgatcgt gccggccgcg agcagatcct
     2341 ggaggtccgc ctcgtcgtcg atgtcgatga acgtctcgta gggaatggcg tgtgccgtct
     2401 gcacgccaag cagcagcacc ccggtcgcta gcaacagccg cttttgcact gcacccaaaa
     2461 cgctatcgca ttacggcgcc tacgtgcacg ccggtggcaa cggattgcac gcgctccaca
     2521 ggccgcgtcg cgccgtcttg gcgtcgagct gcagcgcgtt gaactcctcg acgcgggatt
     2581 gaccgttcgg tgggatgtac agcacgcacg cgaatccgtt ccgcacgagc agcgagttga
     2641 cttcctggcc gtcgacggtg acgtaggcga gcgtgcgccc ataccggtcg gtgcattgct
     2701 cgtcgaagct gagctgcacg gtcttgttga gcacgaggtc ggtgttgtac tgcagcgcgt
     2761 tcggtccgta gcaatcgtcg acagaaccgg ttgtctccgg tgtgtcgacc atcaggtagc
     2821 ggatcttcac gccgctctcg agctcgatcg tgtcgccgtc gatgacgcgc gtgaccttgc
     2881 cctcggtatc gccgcacggc gagccaccac atcccgacgc aaccccgagc gagaagcctg
     2941 caagcagcgc ggcgcgcgtc acatgcacgc gttggctgcc ttcggcgtgc ccttgttcat
     3001 cgccgtgccg tagtcggtga gcgccgggca gaagttcgcg aacgagtcgt tgtccgtcgt
     3061 cgtgagatta gtgggcttga gctgcgcgga cacaccgtcg gcagttgccg tcgacggcca
     3121 gccgtttgcc gtcgtgtaca cgaccgcgtc gtagaccgtc gtgcacgtga ctggtgtgcc
     3181 cgtgcacgtc gtcggatcgc tgacctggat gtcgccgttg ccgttgacga gcgcgacgct
     3241 gaacgtcgca tcgggcgtcg gcaggccacc gttcatcgcc gggtcggcac tgtgcgcgag
     3301 cagcgcgtat gcaccgggtg cgaccggctt gcacgccgcc gagttgatta cgtttggcat
     3361 cgacgcgctc gagacgcggc cgacgccgag gccgttgagg tcgaacgacg cggtgccgac
     3421 gttcgtgact tcgaaccact cctggccggg ctcggccttc gggttcggca tgatctcgga
     3481 gatcacgagc gcgttcgccg cgggcttcac gatcgcgcgc agcgtaccgc cgtcgtcgca
     3541 catgcccggt ggcggcagca tcgtgcactg gccgttgacc atgcccggcg taccgaagtc
     3601 gccgaggccg tacgtcgcgg tcgcgtcgca gaagttcgac gaattgtcgt tcgacgtcgc
     3661 atcggtgaga tcgggatcga gctgcagtgc cttgccggtc gtcgacttcg tccacgagat
     3721 cgcgtcgatc accgtcgatc cggacaggac ctggacgtcg ccgggcgagg tcgccgagcc
     3781 ggccaccatc gagaatccga acgtgccgac tgcaccggta acgccgccgt tcatgctcga
     3841 atcgaccgac ttcgcgaaca cgacgtagtc accggtgtcg acgtggatgc acgtcggcga
     3901 gctgatcgtc gtcggattcg acgtatcgcc tgcgcgatcg agaccgaggc cgttgagatc
     3961 gaacgctgcg agcgccttcg cctcgaacca ttcacccgcg gtgtccgaga ccttcgacgg
     4021 gctcggcatc acctcggtga tcacgagctc gcctggggcc ggcgatacga caggacgcat
     4081 cgagccgcca tcgctgcacg caccggagat gaccggcgcg caatcgttgt cctgacccgg
     4141 cgtgccgaag ttgttcggct cgaactcggt gtcgtggcct tcgcaccagt tcgcgaggtc
     4201 gtcgttcgcg gtgtagtcgg gcggaccgag gttcgacagc tggcgcgagt gaccgctctt
     4261 gacgagctcg tactgcgccg aatcgatctc cttgtcaccg cacttgagcg cgagcttgcc
     4321 gccgtccgaa ttgaagaagt cgccgaggtc ggccgagtag ccgtaatcga cgtacgcggg
     4381 cacgaggtcc gacgtcgagt tgccgagcgt gaagtactgg cccggcgcga tcgtcacgtc
     4441 ggtcatcgtg tgcgactgcg ctttcgaccc gtcgggacgg ctgtgcacga ccgtgaggcc
     4501 ctcgagcgag agcgggcgat cggagttgtt gtagacctcg aaccactcct tgccatcgtc
     4561 ggtgcccgtg ccaccggtcg gtgccgcgta gtcggcgaag acttcggtga tgacgaggtc
     4621 gccggcgagc aggtcgtctt tgcacgggcc gctggagcta tcagggccgc acgcggcgaa
     4681 cgctgcgacc agtgcagcag ctaggcaagc gaagcgcatc tgcatacgat aaatcatcgg
     4741 gtgttgctgc tggttggaat tgctggggga gtgggggccg ggccgggcgg tttagctggc
     4801 gccggtggtt tgcctggagg tacctgagag acccggacct gcgatgcgcg atcgatcacg
     4861 atctggacca cttttttatg gttgtgcggg ttctcgtaga tcgtcgggac accggtgatc
     4921 cagacgaact cgcctttctg ctcggcgata ttcgattgca cgaacacgtc acgatcgaaa
     4981 aacacgagcg gaaaatcgga gaactgttgg cgtgacagcg tcacacgcgt cgggcccttc
     5041 tcgccgatcc gcacgtcgcc gacggtgccg agcacgttga cctctttacc gacgaggtcc
     5101 tcgagctgct tcgtcgcgtc ccagtgcgtg atgtcgacgt agttcgcctt gccttcgccc
     5161 tcggtgcgga actgcgcgac gaagtcaccg cgcgcgttcc accacgcgag ccgctcgtcg
     5221 taatcgggat acgccatcac gccgtccgcc cagatgccgc gcttggcttt gcgtgcttcc
     5281 tcctgcgcct cgacgaattc cttgtggaag cggcgtgact ggccgtactt cgggaagtac
     5341 gggcccatgc ccgcgcgcac ggcctcgatg ttgtagttga cccacgtgcc gttgcggttg
     5401 acgaacacgt acgcgaggta gcgctgatag cgatcgcgga tctcggcagg gtgatcgcgc
     5461 tcgagccgca ccttgtcgga accggagaag aatttctcgg cgaagtgctt cgcgtcctcg
     5521 ccgagcggcg tcgcgaactt cacggggcgc ggtgagttgc cgcgcttcat cgccatgtac
     5581 tgcggccagc cgccttcgaa cgcgcggcga tcaccttcgc tcttgaacgt ctcctcggtg
     5641 tcgacgccga gcagacgcag cgagctgtcg agaccgtcga cgcgaatcgt gtcaccgtcg
     5701 gtgatcttgg tgacgcggaa ctcgccgatg acgaggcccg gcgtctcgag cttgagcagc
     5761 gacttctgcg cggccttgcg cgtgtaccga tcggaatgcc cgccacaccc gacgagcgcg
     5821 agagcggcga tgactgcgag acgcttcatg gacatgctgg gttcgcctgc ctcggagtgc
     5881 cttccggcgt cggattgacg caccagttgg cagggttgtc gttggcgtcg acgtcgggcg
     5941 tgacgccaag cgagtacgtg ccggtgtcgg gcaacacgtc gtagaccgcg cggtcgatca
     6001 gcgtgccgca cgcttcgacg tcgaccgcgg ccgccggcaa gaagccctgg tggaagtcca
     6061 tcgcgaagcc gtagtcgatc gcggccggct tggtgtcgtc gtcgtaggac aggccgagcg
     6121 tgacgtaacc accgccgggg acggtcagcg accggcgcac gatgatcggc acctcggagc
     6181 tgccgtcctt cttgcgaaac cggatcttgg tcccttcgag atcgacggtg gcgcccgacg
     6241 cgttgaacag ctcgacccag atgccgaggg aatcttccgg gctctgctcg ccgcggagct
     6301 cggtgacgat gaggtcgcct cgctcgatcg gcggacactc agcaggagca ggctcgcgga
     6361 cgcagcccac ggccaagacg acggtgagcc cgatcactcg cacccgagta ctgtaacgca
     6421 ggatatgctc gcaggtcatg acgaccggag cacctccgcc tatgcaacct cctgtgggcg
     6481 gtggttatcc gccgtcgacg ccggctgcag cgccaaagcc tgcggatcct gcaaggtctg
     6541 cggcgatcat gctcgtgatc gcggcggtgc tgatcctgat cggcacggtg tcgaagaact
     6601 gggtctcggg cggccgcggc tcgcgtgaca tccatatcgg cccgatgggc ggcgaggcgt
     6661 gcatgagtgg agcctgcgtc gacattccga cgcgcggcat cgacggtgac atcgaagcga
     6721 tcatggtgct cgcgctgatc agtggcttcg cgtcagctgc agcggcgggc accttcggcg
     6781 gtatggcgct cgccggcaag accgacaaga ttccgctgcc accgcgcctc gcgcagatgg
     6841 cgttcggcct cgccgcgttc tcgatgacgt tcttcgtgat ccggatgatc agcgagaagg
     6901 gcgagctcag ctgggcaggg tttcctgcga tcggcggcgt gatcctcgcc ggcgtgggcc
     6961 tcaagaagct gacgccgttc ctcgccgcga aaccctcagt gccgccgggc gcgcagcaac
     7021 agttcgcgaa ccaacagccg tacggcaacc aatcacaacc gatgcagcca tacggccagc
     7081 aatcgcaacc gatgcagcca tacgcgaacc agtcgcagcc gatggcagcg ccgcagcaac
     7141 cgccaccagg cgcagcaccc attcacaact gcccgcgctg cggcacgcag ctgcactggg
     7201 tcgcgcaata tcagcgctgg ttctgtccgc gcgagcagca gtacgtgtga gcgtcgacaa
     7261 gctccgcacg ctcgaggagc tgctcgtgtg gtgtcgcgca caccgcgcgg atctcgtcga
     7321 cgtcgtcgtc caggacgaat tcacgcacga cgtgatcgtt cgggaagctg acgacacgta
     7381 tctgtgcttc gacacgacct gactcggcgc catcaatggc gtctccgggt gggaccacag
     7441 accgaccgcc gacgagctac tcgacgcgcg ggtggcgcgc ggctggacgg cgacgccaac
     7501 cggaacagtc gacggccccg tcgtgctcgg gcatgctact tgtagattcc gatgacgctg
     7561 cgcagtttcg gtctgtgtgg gcttctgctt gctgcgtgca gcgaaggcgg cagcagcgag
     7621 atgcacaccg acgcgcggcc gattcccgat gcgcacgatc agatcgacgg gtcgtcgcag
     7681 gtgccctatc gccatgcgat cgcgctcgac ggcgtcgatg acttcgcgct cgtcgaccaa
     7741 ttcgcgacga cgtctgcgag cttcgaagcg cgcgtgacgt gggacgagaa gaacatctac
     7801 gtcggctact cggggcccga cctcatgacg tccacgagcg acgcgaacac gaagtggctg
     7861 ttcgtgtatc tcgacacgaa gccgggcggc gaagcgcaga gcgagctgta caacacgcag
     7921 cgcgcgacgt tcccg
//
LOCUS       DASUFX010000759        12480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_956850,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000759 DASUFX010000000
VERSION     DASUFX010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12480
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956850"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1016
                     /gene="fsa"
                     /locus_tag="VFV99_30755"
     CDS             372..1016
                     /gene="fsa"
                     /locus_tag="VFV99_30755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX5063814.1"
                     /translation="MKFFIDTADIKEIREAAAMGLVDGVTTNPSLVAKTGRKFRDVLL
                     EICDIVKGPVSAEVVTLTHDEMMKEARELAKLRGNIVVKIPLIPEGLKAVRTCSEEGI
                     KTNVTLCFSATQALLAAKAGASYISPFVGRLDDISTDGMQLIGEIIEIYDNYSFATEV
                     LVASVRHPMHVHQAARMGAHVATCPLSVLLQLTKHPLTDIGLAKFLDDWKKVPQ"
     gene            1013..1444
                     /locus_tag="VFV99_30760"
     CDS             1013..1444
                     /locus_tag="VFV99_30760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5063815.1"
                     /translation="MSDRRAFSVAIYARRGDRVLVIEHRRLKTWLPIGGELEPGETPL
                     EAAVRELREETGLVGMWRPLADALDGVPTGLIGYEEHMAGSKGLHMNFVFVCEIAAGD
                     DVKPNDEFGAWRWVNRAELDRLESPLNVRQFGYVALDARFA"
     gene            1503..2111
                     /locus_tag="VFV99_30765"
     CDS             1503..2111
                     /locus_tag="VFV99_30765"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063816.1"
                     /translation="MRIRQHVNPTDLFFETFRGQKPDLDPARELEVEIGCAEAQFLFE
                     RAEHDRSRHYVGLEIREDLVHWVNNKARVSGAPVQAVFCHAQLHTKLIFPRSAAQRVY
                     VNFPDPWFKRRHHVRRMVDSVLAEQIAYLLRPGGELFVQTDVWDIAIDAMAAFDGDDR
                     FLNVDDEWTFWKRGNPYGVRSWREQNAEETGLPIWRIWYRRL"
     gene            complement(2400..3038)
                     /locus_tag="VFV99_30770"
     CDS             complement(2400..3038)
                     /locus_tag="VFV99_30770"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX5063817.1"
                     /translation="MRKKSRLFAIAEALRARRTGVTAAELAQRFNVTLRTIYRDLEAL
                     QDAGMPIKADRGRGGGYALDKNYQLPPVNFTAREAALLVAMTRFVLEQRIIPFPKAIE
                     RAADKVRAALTTSAQRELLTLVGQLQFVGVPALPVPPAVREAIETAWFESRPLRIVYA
                     KSQWQMSPRIVRIKALVFERSITLLNCVDLEKNDDRQFRIDRIKQAQVLDHA"
     gene            3100..3561
                     /locus_tag="VFV99_30775"
     CDS             3100..3561
                     /locus_tag="VFV99_30775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5063818.1"
                     /translation="MTLDLTRKLPRPIGHHTITPGFSVPHAAKVIDFLQRAFGGETVD
                     RYDGPNGVVFHAEVKIGDSVVMLGDASKDHPALPGGFSLYVDTGEAVDTTYKRALAAG
                     AVSEMEPQNQFYGYRSATVRDVGGNKWTICAVVEDLTRAEIDKRMASMPKQ"
     gene            3630..4289
                     /locus_tag="VFV99_30780"
     CDS             3630..4289
                     /locus_tag="VFV99_30780"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5063819.1"
                     /translation="MKLHYGPLSSSSRRVRITAKLLGINLEEISVDLRDPAHRAELVK
                     LNPNSKVPVLVDGNFVLWESIAIMQYFCDHTPSQKLYPTDARERAEVNRWLSWGQAHW
                     APAIGGISWENVWKKLAIGGAPDPDQVKRQETFFNQFATVLDGHLANRLWLAGETLTL
                     ADIAVGTPLMMTKLARLPLRPYEHVQAWFARIGALPEWQATEPPQLAMLEAASETSRG
                     S"
     gene            4330..6405
                     /gene="uvrB"
                     /locus_tag="VFV99_30785"
     CDS             4330..6405
                     /gene="uvrB"
                     /locus_tag="VFV99_30785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5063820.1"
                     /translation="MANYRGKKARRSDESTPPPPLPARTASSFKLKAPFPPAGDQPKA
                     IRELTEGLKRGDEAQVLLGITGSGKTYSVANVIAATDRPTLIIAHNKILAAQLYGEFK
                     ELFPENAVHYFVSYYDYYQPEAYVPTTDTFIEKDSIVNEEIDRMRHAATFALLSRRDV
                     IIVASVSCIYGIGERETYASMTAELDVGTSIDRDSVLRRLVELQYERNDLDFHRGTFR
                     VRGDTVEVFPAYEADTAIRIEWWGDQIEAMSEIDPLRGGVKRKLERSVIFAASHYATP
                     GDTMRRACESIRQELKVRLEELSRDGKLLERQRLEQRTMYDLESIEQMGFCSGIENYS
                     RHLTGRNAGEPPPTLMDYFPDDYLLIVDESHQTVPQIGAMYNGDRSRKETLVEYGFRL
                     PSALDNRPLRFDEWRQRAKQTIYVSATPAEWELNEAKGIVVEQIIRPTGLLDPEVEVR
                     PVAHQVDDLLAEIRERTGQGDKVLVTTLTKRMAEDLTEYYREIGVKVRYLHSDVDTLE
                     RIQIIRDLRRGEFDVLVGINLLREGLDIPEVSLVGILDADKEGFLRSERSLIQTIGRA
                     ARNIRGRVLLYADKETDSIRDAVGETRRRRGVQELFNKEHGITPKSASRAIMDIQVAE
                     PIPKKGQRSMQLAAIDLKKIDDIDSLRAAIDKLRAEMRQAAADLEFEKAAALRDKARE
                     LEQLELQMR"
     gene            6425..7117
                     /gene="thiE"
                     /locus_tag="VFV99_30790"
     CDS             6425..7117
                     /gene="thiE"
                     /locus_tag="VFV99_30790"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5063821.1"
                     /translation="MHALHVVRASLRRGLYAVLDRDDEQLARTLVGAGAQVLQIRIKP
                     GAVDDIVRVARMARRVCNDAGAHLIVNDRIDIALAVGADGVHLGQTDIPLADACALVG
                     TRLAIGISTHNLDQVRAAVQLAPAYIAYGPVFPTTTKATPDPVQGIPALRAAVKAAGD
                     IPVVAIGGITPVNSRAVYGARAASICAIRAVNSAPDVAAAVRAFRGCSPWLKNTGTYG
                     ILRYGPASVHLE"
     gene            7159..7470
                     /locus_tag="VFV99_30795"
     CDS             7159..7470
                     /locus_tag="VFV99_30795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063822.1"
                     /translation="MTKLGIFASITTIIVSLGIATMTSSDAQAVTPCVTTEFKTELAK
                     SACEKGGQPAAKDAMKKFMKDAKIKSCNACHAKLAPKYELKKDALEQFTKAGGKALPA
                     K"
     gene            complement(7506..8315)
                     /locus_tag="VFV99_30800"
     CDS             complement(7506..8315)
                     /locus_tag="VFV99_30800"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063823.1"
                     /translation="MALKSLLAAVGLAALGGCFWTTTKSEGEALRRDVNALQSKVDSK
                     QQELDTQIKQLNSVLEDASKILKRNTADLGADVEKMSADLRQANGLMVGINTNINDLK
                     VAFDKYRKENDARLDSLEQRVAQIESGKPSANSSPDDLWKLGTQAFEAARYNDALDIY
                     KRLYQTFPTHARADDAAYFRGQAYTNLKDWDKAIAAYQTLVEKYPTSDLADDGLYFAA
                     LAAQQLKNCTEARTYLGLIAQKYSKSNVVKQSKDLDATIKKELKNKAKCSS"
     gene            complement(8306..9049)
                     /locus_tag="VFV99_30805"
     CDS             complement(8306..9049)
                     /locus_tag="VFV99_30805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063824.1"
                     /translation="MGKVSVAIATLAMVVLCACGSKPKQPGCKGDKDCKNAQVCVANK
                     CVECRDDSQCPKGKSCKANACVAKQQCINDEQCPLGQVCQAGSCKPCASDGECGPGGR
                     CEAGACQRPKKCTKNEDCADDEDCTNGLCLKTGAGSNIDAGCQLQTIYFAFDDSSIQP
                     SERDRLEQNNQCMNKNKTQSVFVFGHTDTSGTEEYNIALSERRAQSVADYLSRLGIDP
                     AKLQVVPKGETESTGLGDDKDRRVEFKWR"
     gene            9105..10484
                     /locus_tag="VFV99_30810"
     CDS             9105..10484
                     /locus_tag="VFV99_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5063825.1"
                     /translation="MARSTILVIDDEPNILTTVRRSLELEGYAVEVAGGGAAGLAKLV
                     EHDIDLVLLDVMMPGETGLEVLPKLRAAKPDVMVVMMSGNATIETAVQATKGGAFDFI
                     EKPLSGDKLLITVQNTLAYARLRYENQRLRGRAQTDFAMIGKGAAMRGIFDKVSKTAP
                     TTGRVLITGENGTGKELVARAIHEHSKRADGPFVKLNCAAIPSELIESELFGHEKGAF
                     TGATQQRRGKFELADGGTLFLDEVGDMNPNAQAKVLRVLQENELERVGGGETIKVDVR
                     VVAATNKDLQAEIAASRFREDLYYRLAVVPIELPPLRARREDIPTLVEHFLEQVCADN
                     DRRKKKVASGALTLLMQHDWPGNVRELKNVVERLAILTGDAEVITEADVGDALPRVKA
                     VKSEFARGTPFKDLVAAAERDIIVAALDANDHHVSNTARELQLERSHLYKKMRALGID
                     HRADTEEPD"
     gene            complement(10481..10681)
                     /locus_tag="VFV99_30815"
     CDS             complement(10481..10681)
                     /locus_tag="VFV99_30815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063826.1"
                     /translation="MGSAVAVAMTLDPSTGLASSSIRFAFWFAMIATLLAVALTGVLT
                     IAEYRAARPSLPKAVARSGPRR"
     gene            complement(11090..>12480)
                     /locus_tag="VFV99_30820"
     CDS             complement(11090..>12480)
                     /locus_tag="VFV99_30820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063827.1"
                     /translation="AAASVMLDSADFVVNAIYANNQFLSNDFEAVGLQLAPLSNGTWT
                     TAFREDCSTCNVIARRFDTNGAPVRTELGAGTTQFNVTTTLTTSGTIPAVASSGLTTL
                     IVWDYFDTVGTAQGVACRALNEQGAGTASQLSISTDAADVVTAAPLSNGNFAVTWQSF
                     ITSMQVVRSIIVKPDCTTLTPSPLTVSTVSGSFGARRSHVASNGAAILYAWVVDGDVR
                     VRQGANNGTLVGTEAVLVPKTATDEVDHVRISPWQTGFAVAVRWAASSGMGPGKIEVY
                     RVSATGALMGSPILITDKSGSDFASDKAFGIAQRSDGALMVVWHQCETGPGSCDVYGR
                     ILRPTGVPVGDPYVIATSTGSDQVNPSIIALDDKAFAAAWNDSSGLEPDPSGSAVRAR
                     ILYPVYDDARGVLGATCGASAPGAPDCKEGLACAMGSDNVQRCYQTCTPPSCPGGGTC
                     STVDGSTSACVF"
BASE COUNT         2229 a         4005 c         4091 g         2155 t
ORIGIN      
        1 aagtcgccgg tcgtcgcgtc gtgctcccac agcttcgtgc cggcgggcgg cttgcggtgc
       61 gtgagcgaga acggtgcgac aagcccgatc gacttgggcg gcgcgacctc tttccacttc
      121 gcgactacac ccgatttcac cggtcccgca aacagggcgg ttagctcgag atacgacagc
      181 tcgcgaaagt agcgatcgcg ctcgacgcgc gcaggaacat cgacggtacc gatgcgaatt
      241 gcgtcgatct ggtcagaggc ttcctcgtcg gaggattcct cgtcgttgtt gtcggttgcg
      301 tccgtcagag gggcctcgta tggtgccggc gcacgctacg ttgtcgtcgg cactggtgca
      361 aggaggagcc catgaagttc ttcatcgata ccgcagatat caaagaaatc cgtgaagccg
      421 cggcgatggg cctcgtcgat ggcgtcacga cgaacccgtc tctcgtcgcg aagaccggcc
      481 gcaagttccg cgatgtgctg ctcgagatct gcgacatcgt gaagggcccg gtttctgccg
      541 aggtcgtcac gctcacgcac gacgagatga tgaaagaagc gcgcgagctc gcgaagctgc
      601 gcggcaacat cgtcgtcaag attccgctga tccccgaagg gctcaaggcg gttcggacct
      661 gctccgaaga aggcatcaag acgaacgtca cgctgtgctt ctcggccacg caggcgctgc
      721 tcgcggcgaa ggccggcgcg tcgtacatct cgccgttcgt cggccgcctc gatgacatct
      781 cgaccgatgg catgcagctg atcggcgaga tcatcgagat ctacgacaac tacagcttcg
      841 cgaccgaagt gctcgtcgcg tcggtgcgcc atccgatgca cgtgcatcag gcggcgcgca
      901 tgggcgcgca cgtggcgaca tgtccactgt cggtgctgct ccagctcacg aagcatccgc
      961 tcacggacat cgggctcgcg aagttcctcg acgactggaa aaaagttccg cagtgagtga
     1021 ccggcgcgcg ttctcggtcg cgatctacgc gcggcgtggt gatcgcgtgc tcgtcatcga
     1081 gcatcgccgg ctgaagacgt ggctgccgat cggcggcgag ctcgagccag gcgagacccc
     1141 gctcgaagcc gcggtgcgcg agctgcgcga ggagaccggg ctggtcggca tgtggcggcc
     1201 gctcgcggac gcgctcgacg gcgtgccgac cgggctcatc ggctacgagg agcacatggc
     1261 cggaagcaaa ggcctgcaca tgaacttcgt gttcgtctgc gagatcgctg cgggcgacga
     1321 cgtgaagccg aacgacgagt tcggcgcctg gcgatgggtg aaccgggccg agctcgatcg
     1381 cctggagtcc ccgctcaacg tcaggcaatt cggctatgtg gcgctcgacg cgcggtttgc
     1441 ctagggctca cgaaatgtgg ctgtagcgtc gcctgggctg gtcctggtag acacggctgg
     1501 gtatgcggat ccggcagcac gtcaatccga cggatctgtt cttcgaaacg tttcgaggac
     1561 agaagccgga cctcgatcct gcgcgggagc tcgaggtcga gatcggatgc gccgaagcgc
     1621 agttcttgtt cgaacgtgcc gagcacgatc ggtcgcgcca ctacgtcggc ctcgagattc
     1681 gcgaagacct cgtgcactgg gtcaacaaca aggcacgcgt gagtggcgcc ccggtgcagg
     1741 cggtgttctg tcacgcgcag ctgcacacga agttgatctt tccgcgcagc gcggcgcagc
     1801 gcgtgtacgt gaactttcct gatccgtggt tcaaacgccg tcaccacgta cgccgcatgg
     1861 tcgacagcgt gcttgccgag cagatcgcgt atctgttgcg ccccggtggc gagctgttcg
     1921 tgcagaccga tgtctgggac atcgcgatcg atgcgatggc tgcattcgat ggcgatgatc
     1981 gcttcctcaa cgtcgacgac gaatggacgt tctggaagcg cggcaatccg tacggcgtgc
     2041 ggtcgtggcg cgaacagaat gccgaggaga ccggccttcc gatctggcgc atctggtatc
     2101 gccgcctgta ggagtgcgtg gcgcatagcg ccgatgagtc gcgtctgccg cttcgcggct
     2161 cccagaggaa cgcgaaggcc ccaagacgcc aagaacgcca gaagaaaggt ttcgggatcg
     2221 cgttctgctc gcacgaccgc gtctgcagac cgcgtcgaag accgaccttc tggtggcgtc
     2281 ctcggcggct tggcttcttg gtgttcgatc tggaacacgt tgtgccgcga cgaaggcgcc
     2341 attcggcccc gacggcacag gaccactgcg ctcggaatcg cgcgtttcgg actcgaggtt
     2401 cacgcgtggt cgagtacctg cgcttgcttg atgcgatcga tgcggaactg acgatcgtcg
     2461 ttcttctcga gatcgacgca attgagcagc gtgatcgagc gctcgaatac gagcgccttg
     2521 atccgcacga tgcgtgggct catctgccac tggctcttcg cgtagacgat gcgcagcggc
     2581 cgtgactcga accacgcggt ctcgatcgcc tcgcgcacgg ctggcggcac cgggagcgcg
     2641 ggcacgccca cgaactgcag ctggccgacg agtgtgagca gctcgcgctg tgcggacgtc
     2701 gtgagcgcgg cgcgcacctt gtcggccgcg cgctcgatcg ctttcggaaa cggaatgatt
     2761 cgctgctcga gcacgaagcg cgtcatcgcg accagcagcg ccgcctcgcg tgcagtgaag
     2821 ttgaccggcg ggagctgata gttcttgtcg agcgcgtagc caccgccgcg gccgcggtcg
     2881 gctttgatcg gcatgccggc gtcctgcaat gcttcgagat cccggtagat cgttcgcagc
     2941 gtcacgttga accgctgggc gagctccgcc gcggtcactc ccgtacgacg cgccctcagc
     3001 gcttcggcga tcgcgaacag cctgctcttc ttccgcatga aagctgacaa aaggctgtca
     3061 caaaggggcg ccaagctcca ggtcgaaagg aacaaaccaa tgactctcga tctgacccgc
     3121 aagcttcccc gtcccatcgg ccaccacacg atcacccccg gcttcagcgt cccgcatgcc
     3181 gccaaggtga tcgacttcct ccagcgcgca ttcggcggcg agactgtcga tcgctatgac
     3241 ggcccgaacg gtgtggtgtt ccacgccgag gtcaagatcg gtgactcggt cgtcatgctg
     3301 ggcgacgcga gcaaggatca cccggcgctg ccgggcggct tctcgctcta tgtcgatacc
     3361 ggcgaggcag tcgacaccac gtacaagcgt gcgctcgcag cgggcgccgt ttccgagatg
     3421 gaaccgcaga accagttcta cggctatcgc agcgcgacgg tgcgcgatgt cggcggcaac
     3481 aagtggacga tctgcgcggt cgtcgaagac ctgacgcgcg ccgagatcga caagcgcatg
     3541 gcgtcgatgc cgaagcaatg agcaacgaat gagcaaggac gaccgcattc atcgagcctg
     3601 gtggttgtgg ctcgcgcaag gagcaaacaa tgaaactgca ttacggaccc ctgtcgtcga
     3661 gctcgcgccg cgtacggatc acggcgaagc tcctcggaat caacctcgaa gaaatctccg
     3721 tcgatctgcg tgatcccgcg caccgtgctg agctcgtcaa gctcaacccg aacagcaagg
     3781 tgcctgttct cgtcgacggc aacttcgtgc tgtgggagtc gatcgcgatc atgcagtact
     3841 tctgcgatca cacgcccagc cagaagctgt acccgaccga cgcgcgcgag cgtgccgagg
     3901 tcaatcgctg gctgtcgtgg gggcaggcgc actgggcgcc cgcgatcggc ggcatcagct
     3961 gggagaacgt gtggaagaag ctggcgatcg gtggtgcgcc tgatcccgat caggtgaagc
     4021 gccaggagac gttcttcaac cagttcgcca cggtgctcga cggacatctg gcgaatcggc
     4081 tgtggctcgc gggcgagacg ctgacgctcg ccgacattgc cgtcggcacg ccgctgatga
     4141 tgacgaagct ggcgcggctg ccgcttcgcc cgtacgaaca cgtgcaagcg tggttcgctc
     4201 ggatcggtgc cctgcccgaa tggcaggcga ccgagccgcc gcagcttgcg atgctcgaag
     4261 cagcgtccga aacctcccgg ggcagctgag gcgtgggccg tgcgcgcgaa agacgctaga
     4321 gtccctgccg tggcgaacta ccgcggcaag aaggcccggc gcagcgacga gagcacacct
     4381 ccgccgccgc tacccgcgcg tacggcctcg tccttcaagc tgaaggcgcc atttccgccg
     4441 gccggcgatc agccgaaggc gatccgcgag ctgaccgaag gtctcaagcg cggcgatgaa
     4501 gcgcaggtgc tgctcggcat caccggctcc ggcaagacgt actcggtcgc aaacgtgatc
     4561 gcggcgaccg accggccgac gctgatcatc gcgcacaaca agatcctcgc ggcgcagctc
     4621 tacggcgagt tcaaggagct gtttcccgag aacgccgtcc actacttcgt cagctactac
     4681 gattactacc agcccgaagc gtacgttccg acgaccgaca cgttcatcga gaaggactcg
     4741 atcgtcaacg aagagatcga tcggatgcgt cacgcggcca cgtttgcgct gctgtcgcgc
     4801 cgcgacgtga tcatcgtggc gtcggtctcg tgcatctacg gtatcggcga gcgtgagacg
     4861 tacgcctcga tgacggccga gctcgatgtc ggcacgtcga tcgaccgcga ctcggtgctg
     4921 cgccgcctcg tcgagctgca gtacgagcgc aacgacctcg atttccatcg cggcacgttc
     4981 cgcgtgcgtg gcgacaccgt cgaggtgttc ccggcatacg aagccgacac ggcgattcgc
     5041 atcgagtggt ggggcgatca gatcgaagcg atgagcgaga tcgatccgct ccgcggcggt
     5101 gtgaagcgca agctcgaacg cagcgtgatc tttgcggcct cgcactacgc gacacccggc
     5161 gacacgatgc gccgcgcgtg cgagtcgatt cgccaggagc tcaaggtccg gctcgaagag
     5221 ctgtcccgcg acggcaagct gctcgaacgc cagcgcctcg agcaacgcac gatgtacgac
     5281 ctcgaatcga tcgagcagat gggcttctgc tcgggcatcg agaattactc gcgccacctc
     5341 acggggcgca acgccggcga gccaccgccg acgctgatgg attacttccc cgacgattac
     5401 ttgctgatcg tcgacgagtc gcaccagacg gttccgcaga tcggcgcgat gtacaacggc
     5461 gatcgctcgc gcaaggagac gctcgtcgag tacggcttcc ggctgccgag cgcgctcgac
     5521 aaccggccgc tgcgtttcga cgagtggcgg cagcgcgcga agcaaacgat ctacgtgtcg
     5581 gcgacgccgg ccgagtggga gctcaacgag gcgaagggca tcgtcgtcga gcagatcatc
     5641 cggccgaccg gcctgctcga tcccgaggtc gaagtgcgtc cggtcgcgca tcaggtcgac
     5701 gacctgctcg ccgagatccg cgagcgcacc ggccagggcg acaaggtgct ggtcaccacg
     5761 ctgacgaagc gcatggccga ggacctcacc gagtactacc gagagatcgg ggtcaaggtt
     5821 cgctacctgc actccgacgt cgacacgctc gagcgcatcc agatcattcg cgacctgcgg
     5881 cgcggtgagt tcgacgtgct cgtcggtatc aacctacttc gcgaaggtct cgatatcccc
     5941 gaggtgtcgc tcgtcggcat cctggacgct gacaaggaag gcttcttgcg cagcgagcgc
     6001 tcgctgatcc agacgatcgg ccgcgccgcg cgcaacatcc gcggccgcgt gctgctctac
     6061 gccgacaagg agaccgattc gattcgcgat gcggtcggcg agacccggcg ccggcgcggc
     6121 gtccaggagc tgttcaacaa ggagcacggc atcacgccga agtcggcgag tcgcgcgatc
     6181 atggacatcc aggtcgccga accgattccg aagaagggcc agcgctcgat gcagctcgcg
     6241 gcgatcgatc tgaagaagat cgacgatatc gactcgctgc gcgcggcgat cgacaagctg
     6301 cgtgccgaga tgcgccaagc cgctgcggac ctcgagttcg agaaagcggc tgcgttgcgc
     6361 gacaaggcac gcgagctcga gcaactcgag ttgcagatgc gctgaggcga tgagtaggga
     6421 cccgatgcat gccctgcatg tggttcgcgc gtcgctgcgg cgcggcctct acgccgtcct
     6481 cgatcgcgac gacgagcagc tcgcgcgcac gctggtcggc gcaggcgcgc aggtattaca
     6541 gattcgcatc aagccaggag ctgtcgacga catcgtgcgc gtcgcgcgga tggcgcgtcg
     6601 cgtgtgcaac gacgccggcg cgcacctgat cgtgaacgat cggatcgata tcgcgctcgc
     6661 ggtcggcgca gatggcgtgc acctcgggca aaccgacatt ccgctcgccg atgcatgcgc
     6721 gctggtcggc acccgtctcg cgatcggcat ctcgacgcac aacctcgacc aggtgcgcgc
     6781 agcggtgcaa ctcgcgcccg cctacatcgc atacggtccg gtcttcccga ccacgaccaa
     6841 ggcaactccc gatccggtgc aaggcatccc ggcgctgcgt gccgcggtca aagcggccgg
     6901 ggacattccc gtggtggcaa tcggcggcat cacgcctgtc aactcgcgcg ctgtctatgg
     6961 ggcgcgcgcg gcgtcgatct gcgcgatccg tgcggtcaat tccgcccctg atgtcgccgc
     7021 cgccgttcgg gcgtttcgcg gatgctcgcc atggctgaaa aacactggga cctacggcat
     7081 attgcgctac ggtcctgcct cggttcacct tgaataattc gcggcctacg ccgtcctagc
     7141 tcgacagagg aatccgtcat gaccaagctc ggtatcttcg cctccatcac cacgatcatc
     7201 gtctcgctcg gcatcgcgac catgacctcg agcgacgcac aagcggtcac gccgtgcgtg
     7261 acgaccgagt tcaagaccga gctcgcgaag agcgcgtgtg agaagggcgg tcagcccgcg
     7321 gcgaaggacg cgatgaagaa gttcatgaag gacgcgaaga tcaagagctg caatgcgtgc
     7381 cacgcgaagc tcgcgccgaa gtacgagctc aagaaggacg cgctcgagca gttcacgaag
     7441 gccggcggca aggcgcttcc ggcgaagtaa gctctacgag ctgcagctgg cctgggtggt
     7501 cacagctacg agctgcactt cgctttgttc ttcagctcct tcttgatcgt agcgtcgaga
     7561 tcctttgact gcttcacgac attcgacttg ctgtacttct gcgcgatgag gcccaggtac
     7621 gtgcgcgcct cggtgcagtt cttgagctgc tgcgcggcga gcgctgcgaa gtagaggccg
     7681 tcgtcggcta gatcgctggt gggatacttc tcgacgagcg tctgatacgc ggcgatcgct
     7741 ttgtcccagt ccttcaaatt cgtgtacgcc tggccgcgga agtacgccgc gtcgtccgcg
     7801 cgggcgtgcg tcgggaacgt ctggtagagg cgcttgtaga tgtcgagcgc gtcgttgtag
     7861 cgcgccgcct cgaatgcttg ggtgccgagc ttccacagat catccgggct gctgttcgcg
     7921 ctcggcttgc cgctctcgat ctgcgcgacg cgctgttcga gcgagtccaa gcgcgcgtcg
     7981 ttctccttgc ggtacttgtc gaacgcgacc ttgaggtcgt tgatgttcgt gttgatgccg
     8041 accatgaggc cgttcgcttg gcgcaggtcc gccgacatct tctcgacatc ggcgccgaga
     8101 tccgcggtgt tgcgcttgag gatcttcgac gcgtcttcga gcacgctgtt gagctgtttg
     8161 atctgcgtgt cgagctcttg ctgcttgcta tcgaccttcg attgcaacgc gttgacgtcg
     8221 cgccgcagcg cttcgccttc gctcttcgtg gtggtccaga aacagccgcc aagagctgcg
     8281 agcccgaccg ctgcgagtag tgatttcagc gccatttgaa ctccacgcgg cgatccttgt
     8341 catcgccgag gccagtggac tcggtctcgc ctttcggcac gacctgcagc tttgccgggt
     8401 cgatcccgag ccgcgataga tagtccgcga cggattgtgc acgacgttcg ctgagcgcga
     8461 tgttgtactc ctcggtgccc gacgtatcgg tgtgtccgaa tacgaagacg gactgggtct
     8521 tgttcttgtt catgcactgg ttgttctgct cgagccggtc gcgctcgctc ggctggatcg
     8581 acgagtcgtc gaacgcgaag tagatcgtct gcagctggca gccagcgtcg atgttgctgc
     8641 cggcgcccgt cttgaggcac agcccgttcg tgcagtcctc gtcgtcggcg cagtcctcgt
     8701 tcttcgtgca tttcttcggg cgctggcacg cacccgcctc gcaacgaccg cccggaccgc
     8761 actcgccgtc ggacgcgcag ggcttacacg aaccggcctg gcagacctga ccgagcggac
     8821 actgctcgtc gttgatgcac tgctgcttcg cgacgcaggc gttcgccttg cacgacttgc
     8881 ccttcgggca ctgcgagtcg tcgcggcact cgacgcactt gttcgcgacg cagacttggg
     8941 cgttcttgca gtctttgtcg cctttgcagc cgggttgctt cggcttactg ccgcacgcgc
     9001 agagcaccac catcgcgagg gtggcgatcg ctacagagac cttgcccatg gcctgaaacc
     9061 taaatgggcg cagcacgtta gtcaaatctg gtagggtctg acgcgtggca cgcagcacga
     9121 ttctcgtcat cgacgacgag cccaacatcc tgaccacggt caggcgctcg ctcgagctcg
     9181 aaggctatgc ggtcgaagtt gcggggggtg gtgccgccgg attggccaag ctcgtcgagc
     9241 acgacatcga tctcgtgctt ctcgacgtga tgatgccggg cgaaaccggc ctcgaagtac
     9301 tcccgaagct gcgcgcggcc aagcccgacg tgatggtcgt catgatgtcg ggtaacgcca
     9361 cgatcgagac cgcggttcag gcgacgaagg gcggcgcatt cgacttcatc gagaagccgc
     9421 tgtccggcga caagctgctg atcactgtcc agaacacgct cgcttacgcg cgcctgcgct
     9481 acgagaacca gcgcctgcgc ggccgtgcgc agaccgactt cgcgatgatc ggcaagggcg
     9541 ccgcgatgcg aggcatcttc gacaaggtct cgaagaccgc gccgacgacg ggccgcgtgc
     9601 tcatcaccgg ggagaacggc accggcaagg agctggtcgc gcgcgcgatc cacgagcact
     9661 ccaagcgcgc cgacggtccg ttcgtgaagc tcaattgcgc ggcgatcccg agcgagctga
     9721 tcgagagcga gctgttcggc cacgagaagg gcgcgttcac cggcgccacc cagcagcggc
     9781 gcggcaagtt cgagctcgcc gacggtggca cgctattcct cgacgaggtt ggcgacatga
     9841 acccgaacgc gcaggcgaag gtgctgcgcg tgctgcaaga gaacgagctc gagcgcgtgg
     9901 gcggcggcga gacgatcaag gtcgacgtcc gcgtcgtcgc cgcaacgaac aaagatctgc
     9961 aggccgagat cgcggcgtcg cggttccgcg aggacctcta ctaccgcctc gcggtcgtgc
    10021 cgatcgagct gccgccgctg cgtgctcggc gcgaggacat tccgacgctc gtcgagcact
    10081 tcctcgagca ggtgtgcgct gacaacgatc gccgcaagaa gaaggtcgcg tcgggtgcgc
    10141 tcacgctgct catgcagcac gactggcccg gaaacgtgcg cgagctcaag aatgtcgtcg
    10201 agcgcctcgc gatcctcacc ggcgatgccg aggtgatcac cgaagcagat gtcggcgatg
    10261 cgctaccgcg cgtgaaggcg gtaaagagcg agttcgcccg cggcacaccg ttcaaggatc
    10321 tcgtcgccgc cgcggagcgc gacatcatcg tcgcggcgct cgatgcgaac gatcaccacg
    10381 tgtcgaacac ggcacgcgag ctccagctcg aacgcagcca tctctacaag aagatgcgcg
    10441 ctctcggcat cgatcaccgc gccgataccg aagagcccga ctagcggcga ggcccgctgc
    10501 gtgcgacggc tttcggcaac gacggccgcg cagcgcggta ctccgcgatc gtcagcacgc
    10561 cggtcagtgc gaccgcgagc agcgtcgcga tcatcgcaaa ccagaacgcg aaccggatgc
    10621 tcgagctggc gagcccggtg ctcggatcga gtgtcatcgc gaccgctacc gccgatccca
    10681 cgccacccag caaagcccca cgatagcgag acagatagtg cgctgccgta gaccaagttt
    10741 cgttcacgat gcctctatcg tcggtgatcg cgcgagagcg atcaagccga tcgttcgaat
    10801 cgtcgacgga gatgtgacta aaccgaggtt cggcgccggc gatccgacgt gcacgtgccc
    10861 gtgcacgtgc acgtgtcacg tgtcacgtgc acggagactg gctggcatca agcgtagccc
    10921 ggaacatgcc caagaaggtc ttcgcgcacg gggacgctgc gtcccgattt gttcgcgcaa
    10981 ctacgctcgg tcgggagaag atcgcagaga gcacgtccgc cgttcggcgg agagagcgtg
    11041 cacgtgcacg tgcacgtgtc acgtgtacga agacaggccg cctctgtgac tagaacacgc
    11101 aggccgacgt cgagccgtca accgtcgagc acgtgccgcc gccaggacac gacggcggcg
    11161 tgcaggtctg atagcagcgc tgcacgttgt ccgagcccat cgcgcacgcg agaccttctt
    11221 tgcagtctgg cgcgccgggt gcgcttgccc cgcacgtcgc accgagcacg ccgcgtgcgt
    11281 cgtcgtagac ggggtagagg atgcgggcac ggacggccga gccagacggg tcgggctcga
    11341 ggccgctcga atcgttccag gccgcggcaa acgccttgtc gtcgagcgcg atgatcgacg
    11401 ggttgacctg atcgctgccg gtcgacgtcg cgatcacgta cggatcgccg acggggactc
    11461 cggtggggcg caggatacga ccgtagacat cgcacgagcc cgggccggtc tcgcactggt
    11521 gccacacgac catgagcgca ccgtcggaac gctgcgcgat gccgaacgcc ttgtcggacg
    11581 cgaagtccga gccgctcttg tcggtgatca ggattggtga gcccatcagc gcgccggtcg
    11641 ccgacacccg atagacctcg atcttgccgg gacccatgcc gctgctcgct gcccagcgca
    11701 ccgcgaccgc aaagccggtc tgccacggcg agatgcgcac gtgatcgacc tcgtcggtcg
    11761 ctgtcttcgg gacgagcacg gcctcggtgc cgacgagcgt gccgttgttc gcgccctggc
    11821 gaacgcgcac gtcaccgtcg acaacccatg cgtagagaat cgccgcgcca ttcgaggcaa
    11881 cgtgcgagcg ccgcgcgccg aagctaccgc tcaccgtcga gaccgtgagc ggcgagggcg
    11941 tcagcgtcgt gcagtccggc ttgacgataa tcgagcggac cacctgcatc gaggtgatga
    12001 aggactgcca ggtcaccgcg aagttgccgt tggacagcgg cgctgccgtg acgacgtctg
    12061 ctgcgtcggt ggagatcgag agctggctcg cggtaccggc gccctgctcg ttgagcgcgc
    12121 gacaggcgac gccttgcgcg gtaccgacgg tgtcgaagta gtcccacacg atcagcgtgg
    12181 tcagccccga cgacgcgacg gccggaatcg tgcccgacgt ggtcagcgtg gtggtcacgt
    12241 tgaactgcgt cgtaccggcg ccgagctcgg tgcgcacggg cgcgccgttc gtgtcgaatc
    12301 gccgcgcgat cacgttgcag gtgctgcagt cctcgcggaa tgcggtggtc caggtgccat
    12361 tcgagagcgg cgcaagctgc agcccaacgg cctcgaagtc gttcgagagg aactggttgt
    12421 tcgcgtagat cgcgttgacg acgaagtccg cgctgtcgag catcaccgag gcggcggccg
//
LOCUS       DASUFX010000760         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1790824, whole genome shotgun sequence.
ACCESSION   DASUFX010000760 DASUFX010000000
VERSION     DASUFX010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790824"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFV99_30825"
     CDS             <1..583
                     /locus_tag="VFV99_30825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063828.1"
                     /translation="EAYRDTGSGSPQQLFSVTGWENDTVYYDPPIAFSPGDVLDYHCD
                     YDNPTSDYFFDGFSARHDEMCVTGGVYWRHGDRLPISDEISFGRGIVYTGSKSCSQVQ
                     ACDEAIDYSNTTVHPTAYEQYDICVLSGCQTGAEAYTAFDNCRYTGCKDQCFTTTSDG
                     TVIGLKLTDAACTSCVDAMCAAERDSCGTATCP"
     gene            complement(593..910)
                     /locus_tag="VFV99_30830"
     CDS             complement(593..910)
                     /locus_tag="VFV99_30830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063829.1"
                     /translation="MRAVLVLVVAIGCSSTTNVGERCGQNRPVCDESLSCSSSVPGGY
                     CTTACSSPGSTAQCPDGSVCDDAPLLGLSCLRICDDLGDCGRDDVTCSAVTDSNLKAC
                     KPK"
     gene            complement(938..2413)
                     /locus_tag="VFV99_30835"
     CDS             complement(938..2413)
                     /locus_tag="VFV99_30835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEX5063830.1"
                     /translation="MRSVVALAALLIVIAPAYADTTAQNLAKYHRLRQRLVTDFVSVG
                     TAPGQSEPAPERTDGAGLMKWGDGTIALGFYLGVLATEQYMLANPAQFPGADDGDPSR
                     LASTREELYDALLALERLDTVADASFPSPCTTTPALNGFFVRDDVPADFYSHFPGITT
                     IQSDFVDPTLTNKEESQDQVYHVQHGLALVVALVPASVVVHGKPLRAWAIEQAQRITQ
                     HYAHGDWVIRNPACGNRTVNRGSNTIGYSFGETLAATFVTDGGYVPTTDLLWSTVWDT
                     LRQPANPTYSNESNLHMALAIMAVGDGYQADTPQVIATLAASQDWWLYPLLHRVLHPN
                     AVGFCTTAPDVNAHVRAMLDELPSDGEPACPGPNGPAPHGFTTHNRFIRGKAQAYVGP
                     PGCEGIRYHGLDYMLLHNLYAIATPGTWNGSPSADPCAPVVGDGAPATGDDGGINGDA
                     GIDPTSSPAGCGCRSTEPGATWPLFVIAVAWSVRRRRIRGR"
     gene            complement(2529..2819)
                     /locus_tag="VFV99_30840"
     CDS             complement(2529..2819)
                     /locus_tag="VFV99_30840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5063831.1"
                     /translation="MKLGILALLETKPTTETAVAELLASAATLARQENATVTWYAFRI
                     GPRRFGIFDTFANEAGRKAHLEGRIAQALLGKADELLASPPTLEMVDLVAVK"
BASE COUNT          492 a          934 c          911 g          492 t
ORIGIN      
        1 ggaggcatac cgcgacacgg gcagtggttc gccacagcaa ttgttcagcg tcaccggctg
       61 ggagaacgac acggtctatt acgacccgcc gatcgcgttc tcgcctggcg acgtcctcga
      121 ttaccactgc gactacgaca acccgacgag cgactacttc ttcgacggct tctcggcgcg
      181 ccacgacgag atgtgtgtga ccggcggcgt ctactggcgc cacggcgatc gtctgccgat
      241 cagcgacgaa atctcgttcg gccgaggcat cgtctacacg ggctccaagt cgtgctcgca
      301 ggtccaggcc tgcgacgagg cgatcgatta ctcgaacacg accgtgcatc cgacggcgta
      361 cgagcagtac gacatctgcg tgctgtccgg ttgccagaca ggcgcggagg cttacacggc
      421 gttcgacaat tgccgctaca ccggctgcaa agaccagtgc ttcacgacga cgagcgacgg
      481 taccgtcatc ggcctcaagc ttacggatgc tgcatgcacg tcgtgcgtcg atgcgatgtg
      541 cgcggccgag cgcgacagct gcggcactgc gacgtgcccg tagccgccgg cgtcacttcg
      601 gcttgcacgc cttcaggttc gagtcggtca cagcgctgca cgtgacatcg tcgcggccgc
      661 agtccccgag gtcatcacag attctcaggc acgagaggcc gagcagcggc gcgtcgtcgc
      721 agaccgatcc gtctgggcat tgcgccgtcg atcccggcga cgagcacgcg gtggtgcaat
      781 agccgccggg aaccgacgag ctgcagctca gcgactcgtc gcacacaggt ctgttttgac
      841 cgcaccgctc gccaacattg gtggtgctgc tgcagccgat cgcaacgacg aggacgagta
      901 ccgcccgcat gggctcgatc ttacctcggc gcggtcatca ccgaccgcga atccgccgtc
      961 gccgcacact ccacgcaacc gcgatcacga acagcggcca tgtagctccg ggttccgtgc
     1021 tgcgacagcc acagccagcg ggcgagctcg tcggatcaat gcccgcatcg ccgttgatgc
     1081 cgccatcatc gccggtggcc ggcgctccat cgccaacgac cggcgcacac ggatcggcac
     1141 tgggactccc attccacgtc cccggcgtcg cgatcgcata gaggttgtga agcaacatgt
     1201 aatccagccc gtggtagcga atgccttcgc atccgggcgg accgacgtat gcctgcgcct
     1261 tgccgcggat gaaccggtta tgcgtcgtga agccatgcgg cgccggccca ttcgggccag
     1321 ggcacgcggg ttcgccatcg ctcggcaatt cgtcgagcat ggcgcggacg tgcgcgttga
     1381 cgtcgggcgc cgtcgtgcag aagccaaccg cattgggatg cagcacgcga tgcaggagcg
     1441 gatagagcca ccagtcctga ctcgcagcca gcgtcgcgat gacctgcggc gtatcggctt
     1501 ggtacccgtc gccgacggcc atgatcgcga gcgccatgtg caggttgctc tcgttgctgt
     1561 acgtcgggtt cgccggttgg cggagcgtgt cccaaacggt tgaccacaac agatcggtcg
     1621 tcggcacgta gccgccgtcg gtgacgaacg ttgccgcgag cgtctcgcca aacgaatagc
     1681 cgatcgtgtt gctgccgcga ttgacggtcc ggttgccgca ggcaggattg cgaatcaccc
     1741 aatcgccgtg cgcgtagtgc tgcgtgatgc gctgcgcctg ctcgatcgcc cacgcgcgca
     1801 acggctttcc gtgcacgacg acgctcgccg gcacgagcgc gacgacgagc gcgagcccgt
     1861 gttgcacgtg atagacctga tcctgacttt cttctttgtt cgtcagcgtc ggatcgacga
     1921 agtcggactg gatcgtcgtg atgcccggga agtggctgta gaagtcggcc ggaacgtcat
     1981 cgcgcacgaa gaaaccgttg agcgccggtg tcgtcgtgca cggactcgga aacgacgcat
     2041 ctgcgacggt gtcgagccgt tcgagcgcga gaagcgcgtc gtagagctct tcgcgtgtcg
     2101 atgcgagtcg cgacggatcg ccgtcgtccg cgccaggaaa ctgcgccgga tttgcgagca
     2161 tgtactgctc ggtcgcgagc acgccgagat agaacccgag cgcgatcgtg ccgtcgcccc
     2221 acttcatgag cccggcgcca tcggtgcgtt cgggcgccgg ctcgctctga cctggcgccg
     2281 ttcccacact gacgaagtcc gtcaccagtc gctgacggag ccggtgatac ttcgcgagat
     2341 tctgtgccgt cgtatcggcg tacgctggcg cgataacgat caacagggct gcgagcgcga
     2401 ccaccgaacg catgccgacg tgaatgcccg ctggtgcgcg cgaacgcaac gacgagcacg
     2461 tgacgccacg agaacgttac atctcgcgcg cccgccgaca gaggcccgac cgtacggctt
     2521 tcgcggcctt acttgacggc gacgagatcg accatctcca gtgtcggcgg tgacgcgagc
     2581 agctcgtccg ctttgccgag cagcgcctgc gcgatccgac cttcgaggtg tgccttacgc
     2641 cccgcctcgt tcgcgaacgt gtcgaagatt ccgaaccggc gcggaccgat ccgaaacgcg
     2701 taccaggtga ccgtggcgtt ctcctgacgc gcaagcgttg ccgcgcttgc gagcagctcc
     2761 gcgacggcgg tctcggtggt cggcttggtt tcgaggaggg cgagaattcc gagcttcatg
     2821 gcatgtcct
//
LOCUS       DASUFX010000761         8818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_540843,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000761 DASUFX010000000
VERSION     DASUFX010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540843"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /gene="dctP"
                     /locus_tag="VFV99_30845"
     CDS             complement(<1..466)
                     /gene="dctP"
                     /locus_tag="VFV99_30845"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5063832.1"
                     /translation="MRLTSLLSVAAVAALASAQPARAENVEIRLATLAPSGSPWMEVL
                     NKAQGEIKTKTGGRVTLKYFDGAQMGDERDFVRKIKLGQLDGASVTSIGLAMIDESIR
                     VLELPMMFQSAEEVDYVADKMWPYFQKKFEKKGFKLNDRGDVGWAYFISKNKV"
     gene            complement(466..1398)
                     /locus_tag="VFV99_30850"
     CDS             complement(466..1398)
                     /locus_tag="VFV99_30850"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX5063833.1"
                     /translation="MRLSLLFVSTALVSACSPQDLAVNSTSKVLAAAQPSLQQESDYE
                     LARNAIPGALKTIEGFWVSGFGNGGAKERLERILAEGYCQYGTGFVEDDWELAKFNKD
                     LPAIEYNNDRSTHIFTRCLNFSLRLLGKRWQKEIFGTPEEVAKLLKDTGKGHRFELMF
                     AGAALGGLVNHNLTRIEMISYLGTVQGIMERVIEIDQKSGPPANKAHAALPYLALGMI
                     YSAKGKAMGGEPDKAKAMFEKAFEISGNRFLLAKTFMAYRVGLQTNDQKFFHDNLVEV
                     LNTAPSIWPEQRLANEIAHRRARRYLSHEKELFQ"
     gene            1528..3129
                     /locus_tag="VFV99_30855"
     CDS             1528..3129
                     /locus_tag="VFV99_30855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5063834.1"
                     /translation="MRVLALTATLATGLAAGCDAKRESLHQRRDPGALVVAQAADVIA
                     LDPVRATDSESIEVGELLFEGLVGWKPGTTEIEPRLATQYQVSDDGMTWTFNLRSHVR
                     FHDGTPMDAEAVVFSFERLLVKGHPQALGDGGNYWRAMLSDVERVVAIDPLTVEIRTR
                     RKYAPLLGNLAMYPIVSPTAVKKWGAKFADHPVGTGPFMFDDWIRDSRVVVRRFDGYW
                     GRPAGLNQIVFQVVVDARQRLINLESGSVDLAMSILPDEQPFVELHPDLDLHHAAGND
                     ISYLALNTQRPPFDDVRVRRAANYAVNKEPIVKLGYQGRAIAADTPLPPSMWGYHQPT
                     TRYQYDPVAARRLLAEAVNDHAFDPAQVYRLYAPSTPRPYLPQPERVARFLQASLEQV
                     GIHTELVLLPYPQFRAAVQAGEHDMCVFGWVGDTGDPDNFLYVLFHTKQSSTELGAQN
                     VALYRDKDVDDMLVSAQEASDEATRSALYAAVQDKLASDAPWVPLAHSELVVAGRKEL
                     ERVVLSPTGHPVYSLIRRRAEDQDR"
     gene            3126..5903
                     /locus_tag="VFV99_30860"
     CDS             3126..5903
                     /locus_tag="VFV99_30860"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5063835.1"
                     /translation="MTDDPTITPEPRYSVRKIITDKILKMPRPVETKPPQLRLRLRTK
                     LVIAMAFAALVPVAIVATIATGVILSSLEAGLREDGDRQLTVGLNLILRSVERLGDET
                     VQLAESSDLAYAMHIGPPALEAWFAREGAHVPSARVQLVDAKGAIVFDRVIGGNDKRF
                     IDVGIKPDDPMVQAGQTWTRTVTLVAVGDRVIVRASSPVVDTSLSLLGVAILSLPLDG
                     DFADSIKGALSADVLIGGTAGRLAVTFRSSLGRRAEPIVLSDQDRAVVLDRERVTRDL
                     DVTFEKGGHEGNYTLAATALLDNHDKPVGIVAVAVDRGPLAATKALAIRSLIIGGLGA
                     LAFALLLALFWSRRLGAPIAELHRGAIAVSRGDLDHRIDIPGGDELTDLADAFNQMTS
                     TLKDNQARLAARMKEIVALHDAGRAVSSVIDLDQVSRKIVDAVARTFDVQLATLWLVE
                     GTKLRASAARARRPDVSAVLATDEALQAAEALRDIVEEVRDGRSLVRTARGSVPGAGQ
                     DSVIAGPIVALPLERKNRVVGVLAVARAEQAREFSDADLNLLTTFADQAGAAVENAQL
                     YQEVRGASEELEKKVRLRTAELTAINTELGKALADLRETQAQLVLSERMAGLGLLVAG
                     VAHEINSPTAAIRGSIDGLATAAARVARHNLPPAVDAMVESLGPMLAERPLITGLFAR
                     KVARELATVLETTGIADTQTIAAELADLNATQDDVMRFVTALGHERALAPRAIAALTD
                     HVYLHRTASTVRHAVGQIQRIVGALKSYSHLDQQATRTDADLHEGLETTLVLLHHALR
                     DIVIERRYGVLPRVPVFVDELNQVWTNLISNAQQALTGKGTIAIETLVDGAWALVRVI
                     DDGPGVPADALPRIFEPFFTTKPKGEGTGLGLGIARQIVAKHGGEMKCESEPGRTVFE
                     VRLPIEPGASA"
     gene            5900..6964
                     /locus_tag="VFV99_30865"
     CDS             5900..6964
                     /locus_tag="VFV99_30865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5063836.1"
                     /translation="MSRKEVIVCVDDEEGVLRVLRAQLGARFGHEAQVLTARSGDESV
                     ALFDELTRDGESIALVIADQIMPGMKGVELLEIVNRRLPTTMKILLTGQAGLDAVIDA
                     INRAHLNQYISKPWDETAILLAVENLLRQYRLVHENQQLIASLSAKNQALLEMNRELE
                     AKIHERTHELAEANTRLAQLAVTDGLTGLYNHRHLHERLSLEVERSQRSGLPLSLLML
                     DVDHFKQFNDTFGHPAGDEVLRQLARVLNDTRRANDVVARYGGEEFAVILVDTAKFTA
                     AKVAERVRERIAAHDFSDAAQRAGKISVSIGVATFPDDGVDAEGLVRSADTALYAAKR
                     AGRNRVVLYTDVMSSTPSVG"
     gene            6990..8000
                     /locus_tag="VFV99_30870"
     CDS             6990..8000
                     /locus_tag="VFV99_30870"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5063837.1"
                     /translation="MSVEDIAVVGAGSYGSCLAMLFGRAGHRVSLWARNPETAAQMQE
                     SREAQAYLPGHKLPDSVKVTADLEAAVKGKRFIVGVTPSHGIRDVFGRAAQWLDPDAI
                     VINASKGLEESSLKTIDGVYGDIFPARIAERATYLSGPTFATEIAAGMPAAIVLAGRS
                     DETTQLAQAALTTLHFRIYRTEDVIGVLMGGALKNVIAIGAGISDGLGFGSNTRVALM
                     TRGLAEITRVGVAMGANPMTFAGLSGMGDLVLTCSGDASRNRRVGLALGQGKKMADIL
                     AEMRQVAEGVKTAKVAKQLAEKIGVDAPIIDAMHAIIHEGVPVRDAITRLLSRPTRSE
                     RD"
     gene            8006..>8818
                     /locus_tag="VFV99_30875"
     CDS             8006..>8818
                     /locus_tag="VFV99_30875"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5063838.1"
                     /translation="MLGYVLDDIFVQHRAPSGHPERPARAEAIRDALLAANIGSRGTH
                     IATRMATDHELAAVHGGAYLTQLERLVPGKQGWLDPDTYYSPGTWDAARAAAGSASEL
                     ATRVLSGELTQGLAVVRPPGHHATRDQAMGFCLLNNVAVAAAAARAAGAARVAIVDWD
                     VHHGNGTQDIFWNDPNVLYTSVHQFPYYPGSGAPTEIGGTDARGATINVGLPAGSTDA
                     DYLAAFDHVFIPALARFKPDLLIVSAGFDPFEADPLAGMRVTHNGFAAMAQRL"
BASE COUNT         1466 a         2972 c         2917 g         1463 t
ORIGIN      
        1 cgaccttgtt cttcgagatg aagtacgccc agccaacgtc accgcggtcg ttgagcttga
       61 agcccttctt ctcgaacttc ttctggaagt acggccacat cttgtcggcg acgtagtcga
      121 cctcctcggc cgactggaac atcatcggca gctcgagcac gcgaatcgac tcgtcgatca
      181 tcgcgagacc gatcgaggtg accgacgcgc cgtcgagctg gccgagcttg atcttgcgga
      241 cgaagtcgcg ctcgtcgccc atctgcgcgc cgtcgaagta cttgagcgtg acgcggccgc
      301 cggtcttggt cttgatctcg ccttgcgcct tgttgaggac ctccatccac gggctacccg
      361 acggagcgag cgtcgcgagg cgaatctcga cgttctccgc gcgagcgggc tgagccgacg
      421 cgagggccgc tacggcggcg acagagagca gactggtcaa cctcatcatt ggaatagctc
      481 cttctcgtgc gagagataac ggcgcgcccg acgatgggcg atttcatttg caagacgttg
      541 ctccggccag atcgatggcg cggtgttgag aacctcgacg aggttgtcgt ggaagaactt
      601 ttggtcgttg gtctgcagac cgacgcgata cgccatgaag gtcttcgcga gcaggaacct
      661 gttcccgctg atctcgaacg ccttctcgaa catggccttc gccttgtccg gctcgccgcc
      721 catcgccttg cccttggccg aatagatcat gccgagcgcg aggtacggca gcgccgcgtg
      781 cgccttgttc gccggcgggc cgctcttctg atcgatctcg atgacgcgct ccatgatccc
      841 ctgcacggta ccgaggtacg agatcatctc gatgcgcgtc aggttgtgat tgacgagacc
      901 gccgagcgcg gcgcccgcga acatcagctc gaagcggtgc cccttgccgg tgtccttgag
      961 cagcttcgcg acctcttcgg gcgtcccgaa gatttccttc tgccagcgct taccgagcag
     1021 cctcagcgag aagttcaggc agcgggtgaa gatgtgggtc gagcgatcgt tgttgtattc
     1081 gatcgccggc aggtccttgt tgaacttcgc cagctcccag tcgtcctcga cgaagccggt
     1141 gccgtactgg cagtagccct ccgcgaggat ccgctcgagc ctctccttgg caccgccgtt
     1201 accgaagccg ctgacccaga agccctcgat cgtcttgagc gcgcccggga tcgcgttgcg
     1261 cgcgagctcg tagtcagatt cctgctgcag gctcggctgc gctgcggcga ggaccttcga
     1321 ggtcgaattg actgcgagat cctgcgggct gcacgcggaa acgagcgcag tcgaaacaaa
     1381 tagtagagac aggcgcattg gacggaagct ccccttcgac ggtggcgtgg gtcccagatt
     1441 caccctggac tgcacgttgg tttggggacg ctaagtcagt gagaacccag agtcaattgg
     1501 ccggggcgaa tgctacattc ggccgtaatg agggtattgg cgctgacggc gaccctcgcg
     1561 acgggactcg ccgcgggctg cgatgccaag cgcgagtcac tgcatcagcg ccgcgacccg
     1621 ggtgcgctcg tcgtcgcgca agccgccgac gtgatcgcgc tcgatccggt tcgcgcgacg
     1681 gacagcgagt cgatcgaggt cggcgagctc ttgttcgagg gcctcgtcgg ctggaagccg
     1741 ggcacgaccg agatcgagcc gcggcttgcc acccagtacc aggtctccga cgacggcatg
     1801 acgtggacgt tcaacctgcg ctcgcacgtc cgcttccacg acggcacgcc gatggatgcc
     1861 gaggccgtcg tgttctcgtt cgaacgtcta ctggtcaaag gccacccgca ggcgctcggc
     1921 gacggtggca actattggcg agccatgctc agcgacgtcg agcgcgtcgt cgcgatcgat
     1981 ccgctgacag tcgagatccg cacccggcga aagtacgcac cgctgctcgg caacctcgcg
     2041 atgtacccga tcgtgtcccc cactgcggtg aagaagtggg gcgccaagtt cgccgaccac
     2101 ccggtcggca ccgggccgtt catgttcgac gactggatcc gcgactcgcg cgtcgtcgtc
     2161 cgccgcttcg acgggtactg gggacgtccc gccggcctga accagatcgt gttccaggtc
     2221 gtcgtcgacg cgcgccagcg tctcatcaac ctggagtcag gctcggtcga cctcgcgatg
     2281 tcgatccttc ccgacgagca gcccttcgtc gagctgcatc ccgatctcga tcttcatcac
     2341 gctgccggta acgacatctc gtatctcgcg ctcaacaccc agcggccgcc gttcgacgac
     2401 gtccgcgtgc gccgcgccgc gaactacgcc gtcaacaagg agccgatcgt gaagctcggc
     2461 taccagggca gggcgatcgc tgcggacacg ccgctgccgc cgtcgatgtg ggggtaccac
     2521 cagccgacga cgcgctacca gtacgatccg gtcgcggcgc ggcgcctgct cgccgaggcg
     2581 gtgaacgacc acgcgttcga cccggcgcaa gtctaccggc tgtacgctcc gtcgacgccg
     2641 cgtccgtatc tgccgcagcc cgagcgcgtc gcgcgctttc tccaggcgtc gctcgaacag
     2701 gtcggcatcc acaccgagct cgtgctgctg ccgtatccac agttccgcgc cgcggtgcag
     2761 gccggcgaac acgacatgtg cgtgttcggc tgggtcggcg acaccggcga tccggacaac
     2821 ttcctctacg tgctgtttca cacgaagcag tcgagcaccg agctcggcgc gcagaacgtc
     2881 gcgctgtatc gcgataagga tgtcgacgac atgctcgtgt ccgcgcagga ggcctccgac
     2941 gaggcaacgc gcagtgcgct ctacgccgcc gtgcaggaca agctcgcgtc cgacgcgccg
     3001 tgggtaccgc tcgcgcactc cgagctcgtc gtcgccggcc gcaaggagct cgaacgcgtc
     3061 gtgctgtcgc cgaccggtca ccccgtttac tcgctgatcc gtcggcgcgc ggaggaccag
     3121 gaccggtgac cgacgacccg acgatcacgc ccgagcctcg gtactcggtg cgcaagatca
     3181 tcaccgacaa gatcctcaag atgccgcggc cggtggagac caagccgccg cagctcaggc
     3241 tccggctgcg cacgaagctc gtcatcgcga tggcgttcgc ggcgctcgtg ccggtggcga
     3301 tcgtcgccac catcgcgacg ggcgtgatcc tgtcgagcct cgaagccggt ctgcgcgaag
     3361 acggcgatcg acagctcacc gtcggcttga acttgatcct gcgctcggtc gagcggctcg
     3421 gcgacgagac ggtgcagctc gcggagtcga gcgacctcgc gtatgcgatg cacatcggtc
     3481 cgcctgcgct cgaggcgtgg ttcgcgcgcg agggtgcgca cgtgccatcg gcgcgcgtgc
     3541 agctcgtcga tgccaagggt gcgatcgtgt tcgaccgcgt gatcggcggt aacgacaagc
     3601 ggttcatcga cgtcggcatc aagcccgacg atccgatggt ccaggccggc cagacctgga
     3661 cgcgcaccgt gacgctcgtc gccgtcggcg atcgcgtgat cgtgcgcgcg agctcgccgg
     3721 tcgtcgacac gagcttgtcg ctgctcggcg tcgcgatcct gtcgctgccg ctcgacggtg
     3781 atttcgccga tagcatcaag ggcgcactgt ctgccgacgt cttgatcggc ggcaccgcag
     3841 gccggctcgc ggtgacgttc cgcagctcgc tcggccgccg cgctgaaccg atcgtgctgt
     3901 ccgaccagga ccgcgccgtc gtgctcgacc gcgagcgcgt gacgcgcgac ctcgacgtca
     3961 cgttcgaaaa gggcggccac gagggcaact acacgctcgc ggcgaccgcg ctactcgaca
     4021 accacgacaa gccggtcggc atcgtcgcgg tggccgtcga tcgtgggccg ctcgctgcga
     4081 cgaaggcgct cgcgattcgc tcgctgatca tcggtgggct cggtgccctc gcgttcgcgc
     4141 tgttgctcgc gttgttctgg tcgcgtcgcc tcggcgcgcc gatcgccgag ctgcatcgcg
     4201 gcgcgatcgc ggtgtcgcgc ggcgatctcg atcaccgcat cgacatcccg ggtggcgacg
     4261 agctcaccga cctcgccgat gcgttcaacc agatgacgtc gacactgaaa gacaaccagg
     4321 cccgcctcgc cgcgcgcatg aaggaaatcg tcgcgctcca cgatgcaggc cgcgcggtgt
     4381 cgtcggtcat cgatctcgat caagtgtcgc gcaagatcgt cgacgcggtc gcgcgcacgt
     4441 tcgatgtcca gctcgcgaca ctctggctcg tggaaggcac caagctccgc gcgtctgcgg
     4501 cacgtgcccg gcgcccggac gtctccgccg tgctcgcgac cgacgaagca cttcaggcgg
     4561 ccgaagcgct gcgcgacatc gtcgaggaag tccgcgatgg ccgttcgctc gtccgcaccg
     4621 cacgagggtc ggttccggga gcgggccagg attcggtgat cgcaggcccg atcgtggcgc
     4681 tcccgctcga gcgcaagaac cgcgtcgttg gcgtgctcgc ggtcgcacgt gccgagcaag
     4741 cgcgcgagtt ctccgacgcc gacctcaact tgctcacgac gttcgccgat caagccggcg
     4801 ctgccgtcga aaacgcgcag ctctatcagg aagtccgcgg cgcgagtgaa gagctcgaga
     4861 agaaggtgcg cctgcgcacc gccgagctga ctgcgatcaa caccgagctc ggcaaggcgc
     4921 tcgccgattt gcgcgagacc caggcacagc tggtgttatc ggagcgcatg gctggtctcg
     4981 gcttgctggt tgccggcgtc gctcacgaga tcaactcacc gaccgccgcg atccgcggct
     5041 cgatcgacgg cctcgccacc gctgccgcgc gcgtcgcgcg tcacaacctc ccgcccgcgg
     5101 tcgacgcgat ggttgaatcg ctcggtccca tgctcgccga gcggccgctg atcaccggcc
     5161 tcttcgctcg gaaggtcgcg cgcgagctcg cgacggtgct tgaaacgacc ggaatcgccg
     5221 atacgcaaac gatcgcggcg gagctggcgg acctcaacgc cacgcaggac gacgtcatgc
     5281 gctttgttac agcgctcggt cacgagcgtg ctctcgcacc tcgtgcgatt gcggcgctaa
     5341 ccgatcatgt ctacttgcat cgcactgcgt cgaccgtgcg ccacgcggtt ggccagatcc
     5401 aacgcatcgt cggtgcgctg aagagctact cacacctcga ccaacaggcg acacgcaccg
     5461 acgccgatct gcacgaggga ctcgagacga cgctggtgct cttgcaccat gcgctgcgtg
     5521 atatcgttat cgagcgtcgg tacggggtgt tgccacgtgt accggtgttc gtggacgagc
     5581 tgaaccaggt gtggacgaac ttgatatcga acgcccagca ggcgctcacc ggcaagggca
     5641 cgatcgcgat cgagacactg gtggatgggg cgtgggcgct ggtccgcgtg atcgatgacg
     5701 gtccgggtgt gcccgccgat gcgttgccgc gcatcttcga gccgttcttc acgacgaaac
     5761 cgaagggcga gggcaccggt cttggccttg gcatcgcgcg tcagatcgtc gccaagcacg
     5821 gtggcgagat gaaatgcgaa tcggaaccgg ggcgcaccgt gttcgaggtg cggctcccga
     5881 tcgaaccggg ggccagcgcg tgagtcgcaa ggaagtcatc gtttgcgtcg acgacgaaga
     5941 gggcgtgctc cgcgtgctcc gcgcccagct cggtgcacgg ttcggtcacg aagcgcaggt
     6001 gctgaccgcg cgctcgggtg acgaatcagt cgcactgttc gacgagctga cgcgcgacgg
     6061 cgaatcgatc gcgctcgtga tcgccgacca gatcatgccc ggcatgaagg gcgtcgagct
     6121 gctcgagatc gtcaaccggc ggctgccgac gacgatgaag atcctgctca ccggccaggc
     6181 cggtctcgat gccgtgatcg acgcgatcaa ccgcgcgcac ctgaaccagt acatctcgaa
     6241 gccatgggac gagaccgcga tcctgctcgc cgtggagaac ctcctgcgcc agtaccgcct
     6301 cgtccacgag aaccagcagc tgatcgcgtc gctgtcggcg aagaaccagg cgctgctcga
     6361 gatgaaccgc gagctcgaag cgaagatcca cgagcgcacg cacgagctcg ccgaagcgaa
     6421 cacacgcctc gcgcagctcg cggtcaccga cggtctcacc ggcctgtaca accaccgcca
     6481 cctccacgag cggctgtcgc tcgaggtcga gcgcagtcag cgcagcggat tgccgctgtc
     6541 cctgctcatg ctcgacgtcg atcacttcaa gcagttcaac gacacattcg gccacccggc
     6601 cggcgacgaa gtgctgcgcc aactcgcgcg cgtgctcaac gacacccgtc gcgccaacga
     6661 cgtcgtcgcg cgctacggcg gcgaagagtt cgccgtgatt ctcgtcgaca ccgcgaagtt
     6721 cacggccgcg aaggtcgccg aacgcgtgcg cgagcgcatc gccgcacacg acttctcgga
     6781 cgccgcacag cgtgctggca aaatctcggt ctcgatcggc gttgcgacgt tccccgatga
     6841 cggcgtcgat gccgaagggc tggtccgctc ggccgacacc gcgctgtacg cggcgaagcg
     6901 tgccggccgg aaccgggtcg tgctgtacac ggacgtcatg tcgagcacgc cgtccgtcgg
     6961 ctgagcgtga tagggtcact cccgcccaaa tgagcgtgga agacatcgcc gtcgtcggcg
     7021 ccggcagcta cggcagctgc ctcgcgatgt tgttcggccg cgcgggtcat cgcgtcagct
     7081 tgtgggcgcg taacccggag acggccgcgc agatgcagga gtcgcgcgag gcgcaggcgt
     7141 acctgccggg acacaagctg cccgactcgg tgaaggtgac ggcggacctc gaggccgcgg
     7201 tgaagggcaa gcggttcatc gtcggcgtca cgccatcgca cggcatccgc gacgtgttcg
     7261 ggcgcgcggc acagtggctc gatcccgacg cgatcgtgat caacgcgagc aagggtctcg
     7321 aagagagctc gctgaagacg atcgacggtg tgtacggtga cattttcccg gcgcgcatcg
     7381 ccgagcgcgc gacgtacctg tcgggcccga cgttcgcgac cgagatcgcg gcgggcatgc
     7441 cggcggcgat cgtgctcgcc ggccgcagcg acgagaccac gcagctcgcg caggcggcgc
     7501 tcacgacgct gcacttccgc atctatcgca ccgaggatgt catcggcgtg ctgatgggcg
     7561 gcgcgctgaa gaacgtcatc gcgatcggcg ccggtatctc cgacggcctt ggctttggct
     7621 cgaacacccg cgtcgcgctg atgacgcgcg ggctcgccga gatcacgcgt gtcggcgtgg
     7681 cgatgggcgc gaacccgatg acgttcgccg gactgtcggg catgggcgac ctcgtcctga
     7741 cgtgctccgg cgacgcgtca cgtaaccggc gcgtcggcct cgcgctcggc cagggcaaga
     7801 agatggccga catcctcgcc gagatgcgcc aggtcgctga gggtgtaaag acggcgaaag
     7861 tggcgaagca gctcgctgag aagatcggtg tcgatgcgcc gatcatcgac gcgatgcacg
     7921 cgatcattca cgaaggcgtg ccggtgcgtg acgcgatcac gcgcctgctg tcgcgcccga
     7981 cgcggtcgga acgcgactga cagccatgtt gggctacgtc ctcgacgaca tcttcgtgca
     8041 gcaccgcgca ccgtcggggc atcccgagcg tccggcacgc gccgaagcga ttcgggatgc
     8101 gttgctcgcg gcgaacatcg gatcgcgcgg cacgcacatc gcgacgcgga tggcgaccga
     8161 tcacgagctc gccgcggtgc acggcggtgc gtatctcacg caactcgagc ggctcgtgcc
     8221 cggcaagcag ggctggctcg atccagacac gtactactcg ccagggacgt gggatgcggc
     8281 gcgtgctgca gccggctcgg cgagcgagct cgcgacgcgc gtgctgtcgg gcgagctgac
     8341 gcaaggcctc gcggtcgtgc ggccgccagg ccatcacgcg acgcgcgatc aggcgatggg
     8401 cttctgcctg ctcaacaacg tcgcagtagc ggcggcagca gcgcgtgcag cgggtgcggc
     8461 tcgtgtcgcg atcgtcgact gggatgtcca ccatgggaat ggaacgcagg acatcttctg
     8521 gaacgacccg aacgtgctgt acacgagcgt gcaccagttc ccgtattacc cgggcagcgg
     8581 tgcaccgacg gaaatcggcg gcaccgacgc gcgcggcgcg acgatcaacg tcggcttgcc
     8641 ggcgggcagc accgacgcgg attacctcgc agcgttcgat cacgtgttca tcccggcgct
     8701 cgcgcggttc aagcccgatc tgttgatcgt gtcggccggc ttcgacccgt tcgaagccga
     8761 tccgcttgcc ggcatgcgcg tcacccacaa cggcttcgcc gcgatggcgc agcggctg
//
LOCUS       DASUFX010000762         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_678687,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000762 DASUFX010000000
VERSION     DASUFX010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678687"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VFV99_30880"
     CDS             complement(<1..1110)
                     /locus_tag="VFV99_30880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063839.1"
                     /translation="MTAPGGDDDTIASGEVQPHAETVRSADGDLGSAQTIGSGAAPLA
                     TTKQELPVVPFESYVRIDEYARGGLGRIIRAKDERTGRFVAIKEMLADNADAAARFVR
                     EAMVTANLQHPAIVPVYEIGQWPDGKPFYAMKLVRGRALNDAIAATADLDGRLALISY
                     VAAVADALAYAHGERVIHRDLKPHNVLCGAFGETVVIDWGLARRLDEKETAPAVHRST
                     SPAPGETHVGAVMGTPGYMPPEQARGERADQQSDVYAIGAILYHVLVGRPPHGGADLA
                     TLLDRVKNATPEKLPPEVPRDLAAIVERAMARDRAMRYPTAAELASDLRRFMTGQLVL
                     AHHYTRRQRLARFVSRNRGGVAIAGAALAILGIVAT"
     gene            complement(1233..1829)
                     /locus_tag="VFV99_30885"
     CDS             complement(1233..1829)
                     /locus_tag="VFV99_30885"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5063840.1"
                     /translation="MTAKAEPTLTFSTVAEWDAWLAKHHDRSSAVLLRIPKRKGSDLT
                     YARALDAALSWGWIDSQKRALDAAAWLQRFSRRSARSPWSKINRARAEALIASGAMKQ
                     SGLDEVERARADGRWARAYEGSRTAEVPADLATALARNSRARAFFDALDRANRYAILW
                     RVQNAKKPETRARRIEQFVALCAAGKTLHPTRAKTRRG"
     gene            complement(1867..2025)
                     /locus_tag="VFV99_30890"
     CDS             complement(1867..2025)
                     /locus_tag="VFV99_30890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063841.1"
                     /translation="MDAVFARALAKSPNERPSSATELAIALADALGYEAHTATAAAPA
                     APPQPRRA"
     gene            2367..2864
                     /locus_tag="VFV99_30895"
     CDS             2367..2864
                     /locus_tag="VFV99_30895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063842.1"
                     /translation="MRRAFLVDLTTADRFAKDLEVGGIYISGATVNLDDECHVVLRAG
                     TDEIRVSARVVLVNEEGAGFEIEALTHELRNQIRALVDISKHLVSLERRKTLTRALAE
                     SDPMRRRAAGSIAPGTRLGDGRRVAQGSISPIAAFAPTQEDDSLAKKAAAAQIAADDD
                     DDVDD"
BASE COUNT          475 a          989 c         1019 g          515 t
ORIGIN      
        1 cgtcgcgacg atgcccagga tcgccagcgc ggctccggcg atcgccacgc cgccgcggtt
       61 tcgcgacacg aaacgcgcca gccgctgtcg gcgcgtgtag tgatgcgcga gtacaagctg
      121 gccggtcatg aaccggcgta gatccgatgc gagctcggcc gcagtcggat agcgcatcgc
      181 gcgatcgcgt gccatcgccc gttcgacgat ggcggcgagg tcgcgtggca cctccggtgg
      241 caacttctcc ggcgtcgcat tcttgacccg gtcgaggagc gtcgcgaggt cggcgccgcc
      301 atgcggcggt cgccccacga ggacgtgata gaggatcgcg ccgatcgcgt agacatcgga
      361 ctgctgatct gcgcgttcgc cacgggcctg ctccggcggc atgtagcccg gcgttcccat
      421 gacggcgccg acatgcgtct cgccgggcgc ggggctcgtc gatcggtgca cagccggcgc
      481 ggtttccttc tcatcgaggc ggcgcgcgag cccccagtcg atcacgacgg tctcgccgaa
      541 cgcgccgcac agcacgttgt gtggcttgag atcgcgatgg atcacgcgct cgccgtgcgc
      601 gtatgcgagc gcatcggcga ccgcggcgac gtacgagata agtgcgagcc gcccgtcgag
      661 gtccgcggtc gcggctatcg cgtcattgag cgcacgtccg cgcacgagct tcatcgcata
      721 gaacggcttg ccgtcgggcc actggccgat ctcgtagacc ggcacgatcg ccgggtgctg
      781 gagattcgcg gtgaccatcg cttcgcggac gaagcgcgcc gcggcatcgg cattgtcagc
      841 gagcatctct ttgatcgcga cgaagcgccc agtgcgctcg tccttggcgc ggataatccg
      901 accgagcccg ccgcgcgcgt actcgtcgat ccgcacgtag ctctcgaacg gcacgaccgg
      961 gagctcctgc ttggtcgtcg ccaggggtgc cgccccagag ccgatcgttt gcgcgctgcc
     1021 gaggtcgccg tcggcagagc gaacagtctc ggcgtgcggt tggacctcgc ccgacgcaat
     1081 cgtgtcgtcg tctccacccg gcgcagtcat ccggtcgatc taacactatt ccgctgggac
     1141 gatcccaacg cagcgtcctc gtgaattcga gcagaaggat cccgctgtat tcgatggcgc
     1201 cccataaggc gaatagcggc ttcgctcgga catcatcctc ggcgcgtctt cgcgcgcgtg
     1261 gggtggaggg tcttgccagc ggcgcagagc gcgacgaatt gctcgatgcg acgcgcgcgg
     1321 gtctcgggct tcttggcgtt ctgcaccctc cagaggatcg cgtaacgatt cgcgcggtcg
     1381 agcgcgtcga agaaggcacg cgctcgtgag ttgcgcgcga gtgcggtggc cagatccgcg
     1441 gggacctcgg cggtgcggga tccctcgtag gcgcgcgccc agcggccgtc ggcacgcgca
     1501 cgctccacct cgtcgaggcc cgactgcttc atggcgcccg acgcgatgag tgcttcagcg
     1561 cgtgcgcggt tgatcttcga ccaggggctc ctcgcagagc gccgcgagaa gcgctggagc
     1621 caggccgctg cgtcgagcgc gcgcttctgg ctgtcgatcc agccccacga gagtgctgca
     1681 tcgagcgcac gcgcatacgt gagatcgctc ccctttcgct tcggaatgcg caacagcacc
     1741 gcgctcgacc gatcgtgatg ctttgcgagc caagcatccc actcggcgac agtggagaac
     1801 gtgagggtgg gttccgcctt cgctgtcatc agtggcctct cgccgtcgaa ggattgtacc
     1861 cctcgcctac gctcgccgcg gctgcggcgg ggccgccggt gctgcggccg tcgcggtgtg
     1921 cgcctcgtat ccgagcgcat cggcgagcgc gatggccagc tcggtcgcgc tgctcgggcg
     1981 ctcgttcggc gacttcgcga gtgctcgcgc gaacaccgcg tccactgccg ctggtaccgc
     2041 caccagcttg ctcgcgcgcg gcggctcggt gtggcggtgg tggtctgttg tgacggaccg
     2101 cgccggttca ggccgccact cgatcgtccg cgaccgcctg cacgtccgca ctgtggttcg
     2161 cgacgtcgac gatcgaattg taggacgcga tcgagaagcc gagcgcgcga cgcgaaggtt
     2221 tgatctcaaa agaaacgaaa cgtccagact tgaatccgaa gatcgggtgc ggctgcgcgc
     2281 gagtttagag tgctgtctga ctctcgcagg gtgtgcgtgc cgtcgcacat cgctgcggct
     2341 ccacgcgttg ccatacactg agcgaaatgc gacgtgcgtt tctcgtcgat ttgacaactg
     2401 cggatcggtt cgcgaaggac cttgaagtag gcggcatcta catctcgggg gcgaccgtca
     2461 acctcgatga cgagtgtcat gtcgtgttgc gcgcaggcac ggacgagatc cgcgtcagcg
     2521 cacgtgtcgt gctcgtcaat gaggagggag caggcttcga gatcgaggcc ctcacgcacg
     2581 agctgcgcaa ccagatccgc gcgctcgtcg acatctcgaa gcatctcgtc agcctcgagc
     2641 gcaggaagac gctcacacgc gcattggcgg agagcgaccc aatgcggcga cgtgcagcgg
     2701 gctccatcgc gccgggcacg cggctgggtg acggacggcg cgtcgcgcag ggctcgatct
     2761 cgccgatcgc agcattcgct ccgacgcagg aagacgattc gctggccaag aaagcggcgg
     2821 ccgcgcaaat cgcagcagac gacgacgacg acgtcgacga ctagactcat cgtccgcgca
     2881 ttgtcgtgca agagaaacaa aaagcgtccg gaccacgatg gggccggacg ctggttaact
     2941 agtctccttc agttatctac gccgcgagct tggcgttgct gctcgagcgc ttgctgcg
//
LOCUS       DASUFX010000763         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1653296, whole genome shotgun sequence.
ACCESSION   DASUFX010000763 DASUFX010000000
VERSION     DASUFX010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653296"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFV99_30900"
     CDS             complement(<1..268)
                     /locus_tag="VFV99_30900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063843.1"
                     /translation="MRWFVVLAFACGCSVPGGRGGGGDDDDVMIDAPGNGSGSVSVFD
                     PSITNVVVEIDYETGNEPYTGPIVGFGDTFEPTQFNIDRLFAGKK"
     gene            complement(324..737)
                     /locus_tag="VFV99_30905"
     CDS             complement(324..737)
                     /locus_tag="VFV99_30905"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HEX5063844.1"
                     /translation="MRLLAVVLLVAACRPPGYGKEPPPDAAEQDSSMVTIDASIDANV
                     PVCDHGFRVDGFGSASSFWMTGSFTNWAGDPVNGAIAFTLGTDGAWSGSYRFQAGTHQ
                     YKFIVNGTDWILDPTNPNTADDGMGHTNNVYTCVP"
     gene            924..3221
                     /locus_tag="VFV99_30910"
     CDS             924..3221
                     /locus_tag="VFV99_30910"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5063845.1"
                     /translation="MRRALPALAAAVAISMTARPAHAIDPVPSFRYLVTGNGHGFQVF
                     DVSANAVKQYLERPYRYLKANPSNPDGEGIVRRNLAFDTYFGIKVGGAGTWFGGRAPT
                     EIGYVEQSNMIRSALATSGVLAESFYVAPFGYEGNGLVMLLKVTNTSGSAQSVTAYSM
                     HNFKMGSAPNPDVPDANSESIAWDATSQTATENGPGGGVMVYAPIGGADASTCAATAY
                     NTVASGGTLTTQASCSGTDQKNAFGKDLGSIPPGEARWWGVAVLFDADGNASGAKTAW
                     STFLGGKTAEQLYTSVLAELEGWRKPPAPGLSATELAVWRQAETVLRMGQIREAYTDS
                     PRRHNTGMILASLPPGGWHTGWVRDATYAVAALARGGHADEAKAALDFFLNADAGRYP
                     SFVNNQPYRISTVRYYGDGQEEADYSGSPTRNIEIDGWGLVLWAARTFVDSSADTAWL
                     NSMTKKGDTVYDALKSGIAEPIVANLESSGLAIADASIWEVHWGNRQHFLYTTATAAR
                     GLCDMATLARRAGRMDDVARYRQLAEKVTMAMKQNFVDSNRVLAGSLERLAQGNNYRD
                     GATVEAFTWSLIDATDTIGTATLGAMSFLQTPAGGYKRVEGSNDQYDTDEWILIDLRA
                     SAAFRRAGNGPKADQLLDWVTGQATTNYDLLPELYNTRTSSGQIGAYSGSIPMVGYGA
                     GAYQLTMFDRVQLYEHSDCGEKDLGEYPDAGPVLPGDGGTGPGGNGFGGRTGVACACQ
                     GGPGSAGNAVMFAIAGLLVLRRRRR"
     gene            3218..>4654
                     /locus_tag="VFV99_30915"
     CDS             3218..>4654
                     /locus_tag="VFV99_30915"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX5063846.1"
                     /translation="MNVVELRRIGKQFPGVRALHDVSIAIEPGEVLGLVGENGAGKST
                     LIKILGGVYAAGTFKGEVVVGGHAQTFRSTRDARKAGIAVVHQELSLVPAMSIAENLL
                     LGREPARFGIVDGADLEHKARALLARVYGTDLAIDVATPIDRLGVGVQQMIEIARALG
                     EHAKLIVLDEPTAALTEAESEKLFALIRARRAEGTSFIYISHRLGEIEALSDRVAVLR
                     DGELVGLRETKATTEQEIVALMTGDNEALDRARTRRDRAQNTTASAPVLAVANLCVAH
                     PSRVDRNVVDNLSFTVAPGEVVALAGAMGAGRTATLAALFGTARRGMTGTVTVDGKSV
                     TLRKPADAIAAGFAYVPEDRKALGLVLGLSVADNLALSALGRMSRLGVVDNARAEHAA
                     LQRLRDLSIKVPGLGAEVATLSGGNQQKVVIGKWLELAPRVLLLDEPTRGVDVGAKAE
                     IYALIEELTARGHAVVLASSDLPEVVRLA"
BASE COUNT          806 a         1541 c         1567 g          740 t
ORIGIN      
        1 tcttcttgcc ggcgaacaag cgatcgatgt tgaactgcgt cggctcgaac gtgtcaccga
       61 agccgacgat cggccccgtg tacggctcgt tgccggtctc gtaatcgatc tcgacgacga
      121 cgttcgtgat cgacggatcg aacaccgaca cgctgcccga gccgttgccc ggtgcgtcga
      181 tcatcacgtc gtcgtcgtcg ccaccaccgc cccggcctcc cggcaccgag cagccgcacg
      241 cgaacgcgag cacgacgaac caccgcacgg caccagtgta actgcgcttt agcgaaaccg
      301 tccgtcagag ctgcgtgagt gacctaaggc acgcaggtgt acacattgtt cgtgtgcccc
      361 atgccgtcgt cggcggtgtt cgggttcgtc ggatcgagga tccagtcggt gccgttgacg
      421 atgaacttgt actggtgggt cccggcttgg aacctgtacg agccgctcca cgcgccgtcg
      481 gtaccgagcg tgaacgcgat cgcaccattc accggatcgc cggcccagtt ggtgaagctg
      541 ccggtcatcc agaacgaaga agcactgcca aagccatcga cccgaaagcc gtggtcgcag
      601 accggaacat tcgcatcgat gctggcgtcg atggtcacca tcgacgaatc ctgctcggca
      661 gcgtcgggcg gcggctcctt gccatagccg ggagggcggc aggcagcgac gagcaacacg
      721 acggcgagaa ggcgcaccgg gccccgttgt tcaagggaca tgccacggca gatccgcgtg
      781 gtctcgtgca cctcgtgagc acgcgtcgcg attcgcaccg agaccgatca ccgccaggtt
      841 gcgtttcgca acctcgccga cggcgcaggt gcctgaatct tcaggccaga cactgggcat
      901 gaatcgtgga aacgagggcc gcgatgcgtc gcgcccttcc cgctctcgcc gccgcggtgg
      961 cgatctcgat gacggctcgg cctgcgcacg cgatcgaccc ggtcccgtcg ttccgctacc
     1021 tcgtgaccgg caatggccac ggatttcagg tcttcgacgt gagcgcgaac gcagtcaagc
     1081 agtacctcga gcgcccgtac cggtacctga aggcgaaccc gtcgaacccc gacggcgaag
     1141 gcatcgtgcg gcgcaatctc gcgttcgaca cgtacttcgg catcaaggtc ggcggagccg
     1201 ggacatggtt cggcggccgc gcgccgaccg agatcggcta cgtcgagcaa tcgaacatga
     1261 tccgctcggc gctcgccaca agcggcgtgc ttgcggagtc gttctacgtg gcgccgtttg
     1321 gctacgaggg caatggcctc gtgatgctgc tcaaggtcac gaacacgtcg ggcagcgcgc
     1381 agtcggtgac ggcgtactcg atgcacaact tcaagatggg ttcggcgccg aaccccgacg
     1441 tgccggatgc gaacagcgaa tcgatcgcgt gggacgcgac gtcgcaaacc gcgaccgaga
     1501 atggtcccgg cggcggcgtc atggtctacg cgccgatcgg cggcgccgac gcgtcgacgt
     1561 gcgccgcgac cgcatacaac acggtcgcga gcggcggcac gctgacgacg caagcgagct
     1621 gcagcggtac cgaccagaag aacgcgttcg gcaaagacct cggctcgatc ccgcccggcg
     1681 aagcgcgctg gtggggcgtg gcagtgttgt tcgacgccga tggcaacgcg agtggcgcga
     1741 agaccgcgtg gtcgacgttc ctcggcggca agacggcgga gcagctctac accagtgtgc
     1801 tcgccgagct cgagggttgg cgcaagccgc ctgcgccggg cctgtcggcg acggagctcg
     1861 cggtgtggcg ccaagccgag accgtgctgc gcatgggcca gattcgcgag gcgtacacgg
     1921 acagcccacg ccgtcacaac accggcatga tcctcgcgag cttgccgccc ggcggctggc
     1981 acacggggtg ggtgcgcgac gcgacctacg cggtcgcagc gctcgcgcgc ggtggtcacg
     2041 ccgacgaggc gaaggccgca ctcgatttct tcttgaatgc agacgcgggt cgctatccgt
     2101 cgttcgtgaa caaccagccg tatcggatca gcaccgttcg ctactacggc gatggccagg
     2161 aggaggctga ctactcgggt tcgccgacac gcaacatcga gatcgacggc tggggcctcg
     2221 tgctgtgggc ggcgcgcacg ttcgtcgact cgtcagcgga taccgcgtgg ctcaattcga
     2281 tgacgaagaa gggcgacacc gtctacgacg cgctcaagag cggcatcgcc gagccgatcg
     2341 tcgcgaacct cgagtcgagc ggcctcgcga tcgccgatgc gtcgatctgg gaagtgcact
     2401 ggggaaaccg ccagcacttc ctgtacacga ccgcgaccgc ggcgcgcggg ctctgcgaca
     2461 tggcgacgct cgcgcgacgc gccggccgga tggacgacgt cgcgcgctat cgccagctcg
     2521 ccgagaaggt gacgatggcg atgaagcaga atttcgtcga ctcgaaccgc gtgctcgccg
     2581 gttcgctcga acgcctcgcg caaggcaaca actatcgcga cggcgcgacc gtcgaggcgt
     2641 tcacgtggtc gctgatcgat gcgaccgata cgatcggtac cgcgacgctc ggcgcgatgt
     2701 cgtttttgca aacaccggcc ggcggctaca agcgcgtcga aggcagcaac gaccagtacg
     2761 acaccgacga gtggatcttg atcgacctgc gcgcgtcggc tgcgtttcgg cgcgccggca
     2821 acgggccgaa agcggaccag ttgctcgact gggtcaccgg tcaggcgacg acgaactacg
     2881 acctgttgcc cgagctctac aacacacgca cctcgagcgg tcagattggc gcgtactcgg
     2941 gaagcatccc gatggtcggc tacggtgccg gcgcatatca gctgacaatg ttcgatcgcg
     3001 tccagctcta cgagcacagc gactgcggcg aaaaggatct cggcgagtat cccgatgccg
     3061 gtccggtgtt gccgggcgat ggcggcacgg ggcccggcgg caacggcttc ggcggccgca
     3121 ccggcgttgc gtgcgcgtgc cagggtggtc ctggctcggc gggcaacgcg gtcatgttcg
     3181 cgattgcggg gctgctcgtc ctgcggcgga ggcggcgatg aacgttgtcg agctgcggcg
     3241 gatcggcaag cagttccccg gcgtgcgcgc attgcacgac gtctcgatcg cgatcgagcc
     3301 gggcgaagtg ctcgggctcg tcggcgagaa cggcgctggc aagtcgacgc tgatcaagat
     3361 cctcggcggc gtctacgcgg ccggtacgtt caagggcgag gtcgtcgtcg gcggccacgc
     3421 gcagacgttc cgctcgacgc gcgacgcgcg caaggccggc atcgcagtcg tccaccaaga
     3481 gctgtcgctc gtgcccgcga tgtcgatcgc ggaaaatctg ttgctaggcc gcgagcccgc
     3541 gcggttcggc atcgtcgacg gcgccgacct ggaacacaag gcacgcgctc tgcttgcgcg
     3601 agtctatggg acagacctcg cgatcgatgt cgcgacgccc atcgatcgcc ttggcgttgg
     3661 cgttcagcag atgatcgaaa ttgcacgcgc cctcggagag cacgccaagc tgatcgtcct
     3721 cgacgagccg acggcagcgc tgaccgaggc tgagagcgag aagctattcg ctctgatccg
     3781 tgctcgccgt gccgagggca cgtcattcat ctacatctcg caccgtctgg gtgagatcga
     3841 agcgctcagc gatcgcgtag cagtactgcg cgacggcgag ctcgtcggtc tgcgcgagac
     3901 gaaagcgacg accgaacagg agatcgtggc cctcatgacc ggagacaacg aagctctcga
     3961 tcgagctcgc acgcggcgtg accgcgcgca gaacaccacg gcgtcggcac cggtgctcgc
     4021 cgtggccaac ctgtgcgtcg cgcatccgtc gcgcgtcgat cggaacgtcg tcgacaacct
     4081 gtcgttcacg gtcgcgcccg gtgaagtcgt cgcgcttgcg ggtgcgatgg gtgccggccg
     4141 aaccgccacc ctcgccgccc tgttcgggac tgcccgccgc ggcatgaccg gtacggtcac
     4201 tgtcgacggc aagtcggtca cgctgcgcaa gcccgccgac gcgatcgccg ctggcttcgc
     4261 ttatgtcccc gaggaccgca aagctctcgg cctcgtcctc ggcctctcgg tggccgacaa
     4321 tctcgcgcta tcggcgctcg gtcgcatgtc gcgactgggg gtcgtcgaca acgcacgcgc
     4381 cgaacatgcg gcgctccaac gtcttcgcga cctatcgatc aaagtgcctg gtttgggcgc
     4441 cgaggtcgcg accctttccg gtggcaatca gcagaaggtg gtgatcggca agtggctcga
     4501 gctcgcgccg cgcgtgctcc tgctcgacga gcccacccgc ggcgtcgacg tcggcgcgaa
     4561 ggccgaaatc tacgcgctga tcgaagagct gaccgcccgc ggccacgcag tggtgctcgc
     4621 gtcgagcgac cttcccgagg tcgtgcggct cgcc
//
LOCUS       DASUFX010000764         7043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2068821, whole genome shotgun sequence.
ACCESSION   DASUFX010000764 DASUFX010000000
VERSION     DASUFX010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068821"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFV99_30920"
     CDS             <1..486
                     /locus_tag="VFV99_30920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063847.1"
                     /translation="GGRVFGSHYHYTWMKNLIPAWTPTASWGGSYSTMTPDLVDTSHP
                     DGMAFSQWLQAAGASTAPGQVALGVKTYDAGPVNAPTTRWLYASGTNPTTHYMSFKTP
                     VGVPMEQQCGKVVYAGLHVSSTSSVGSDFPAGCTGTFTPDEKALAFLLFDLGSCVDVI
                     F"
     gene            626..1858
                     /locus_tag="VFV99_30925"
     CDS             626..1858
                     /locus_tag="VFV99_30925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5063848.1"
                     /translation="MRGLTQFLVVGLSISMLAACGPNKRGEDDDLPDAPPPDAPCPTT
                     ISGKVFAPNGTLPLYNVTVYIPQTAPGPFTDGVSCTHCSTDLPGGVVTKAISDAEGKF
                     TLENVPPGTDVPVVITTGKWRRQLTVPYVAECVDTPIQDGVFRLPKNRTEGDLPRIAM
                     VTGACDGLACIFSKMGVSASEFGSSSSGPERIIWYNAHGGVSPGTSQPAQTGLWTNLA
                     EMQKFDMVINSCECSEYQTEKTAPDVIRQYADMGGRFFGSHYQYTWMQEKVTQWQGTA
                     AYMDSTIGSSVVSVDTSHPDGMALAKWLSAVGGSTTPGQLMLSTTSQPSETAVNSPTT
                     RWLYDPSYVYYMSFKTPVGVPMEQQCGKVVHAGIHVSESSSVNASFPSGCSTSLTPDE
                     KAMIFLMFDLGSCVDVIF"
     gene            complement(1855..2775)
                     /locus_tag="VFV99_30930"
     CDS             complement(1855..2775)
                     /locus_tag="VFV99_30930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX5063849.1"
                     /translation="MERPSTRQLECLVAVADHGSFRKAAASLGISQPALSAQVQSAED
                     LLGVQVFERDRRSVLVTPAGEDVVGRSRIALESIDAVNDAARRRAEPLVGPLRLGVIP
                     TIAPYWLPALLPVVRKRFPKLELILREDQTARLLAQLSAGQLDLALLAIPVPGDVTTA
                     PIARETFMLAAPRGADVLAKHGKLSERDLEDQTVLLLEDGHCLRDQALAVCERGGAVE
                     SIGVRATSLPTLVQMVAGGLGVTLLPEAAAEALVQPRGPVEVADFTKPPGRTVGLAWR
                     TSSARLREFRLLAEVMSETAEKFLAKIRER"
     gene            2866..3405
                     /locus_tag="VFV99_30935"
     CDS             2866..3405
                     /locus_tag="VFV99_30935"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5063850.1"
                     /translation="MLTIGDQFPAFKATAVVSQEKGKEFLEITDQTYDGKWKVYFFWP
                     KDFTFVCPTEIAAFGKLERDFADREAQLLGVSIDSEYVHLAWRGSHPDLKDLPYPMVA
                     DIKRELSTALGVLDKREGVALRATFIVDPQNVIRFVSVNDLSVGRNPAEVMRVLDALQ
                     TDELCPCNWQKGQNTLNAA"
     gene            3414..3932
                     /locus_tag="VFV99_30940"
     CDS             3414..3932
                     /locus_tag="VFV99_30940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5063851.1"
                     /translation="MTNLDQLKERLPDYARDLRINLGVIGSSTALSPVQAWTIALTSA
                     ATTRNRDLVAAVEADAAAHVEPTALTAARGAAAIMGMNNVYYRFLHFMGEAPPYGHMP
                     ARLRMQVIGNPGIDKLDFELACLAASAIAGCEACVRSHEKAVRERGGTKEQVQDAVRI
                     AAVLNGVAVALG"
     gene            3985..4512
                     /locus_tag="VFV99_30945"
     CDS             3985..4512
                     /locus_tag="VFV99_30945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063852.1"
                     /translation="MQPHAPRAVGPKTTNGHNKPDRTLSHGRPAEPLLRVLPIKRWIP
                     QDVHSMLDYAAGVSVASGHFTTDDEAARWASVALGASVIGVSLLTDYRLSLVKLIPIR
                     AHEALDYVWGASCIAAPFVLGYWKKSPLTAITHIAVGATTILGSLVTDYRSYQQQRDD
                     ERRRSQADLQPIMPT"
     gene            complement(4499..5482)
                     /locus_tag="VFV99_30950"
     CDS             complement(4499..5482)
                     /locus_tag="VFV99_30950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063853.1"
                     /translation="MMWPFDRGGGVLALYVATLALHAVFIGYVVAGTGYVLLQSLRRV
                     DDPVAATVRDRLPFMLGAGITAGVAPLLFVQLLHQTRFYTANLLLGPRWMAVVPALIV
                     GFYALYLAKMSERFRKLALALGLVCFAFVAWSWSELHELMKADPEWHAFYAAGKRVFL
                     AESIAPRFVIFGGAMTTTFAMIAAWSAADAGRKRLAVLAIAARIVSVGGAVWLWRSGF
                     AVDGAARAWLVVLVVALVVELGGWLWLWRQPSVGDPRRGTERALALVTGAGTGAIVAA
                     IVVRETPRVALIEPTHALASTAGGAVVFALALAVGVAAITYVIRLARSTSA"
     gene            complement(5479..6990)
                     /locus_tag="VFV99_30955"
     CDS             complement(5479..6990)
                     /locus_tag="VFV99_30955"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5063854.1"
                     /translation="MAPIPFYPVNDFGPLMKGMVIGGVGILHVFLAQFAIGGGMLLWY
                     FERLAQRGKERDARTFVDGYFQYLVLISFVVGALTGVGIWFTTIQVGARTIGLMIDQF
                     HWLWATEWVCFSVEVAAGYAFVRVGRQLGDRARLRLLAIYAVAAWLSLFFINGILSWQ
                     LTPGGSIVDSAMGALGYPTSGRTWAGFFNPSFWPSLLFRTVVAMTLAALVACIVINTL
                     DLDRERRAALIRRAARFTAPMAAMPLLALWFLAVIPPDSRSWLLGGSMAMTMFVVIAG
                     GASLLIGGYALIGLLAQRLYINGATATLLLALAFGATAAGEFVREGARKPYTVRGVLY
                     SSSIAPADVTAMRVRGATAGDPYPLRDEARYPTDQLKRGAKVHRTLCDACHTIDGANG
                     LVELSRTWTDDQLRLNVAKLQHTKSFMPPFAGNAEDVEAIVQLIRWEMQGAPASWPTS
                     AVPETLAQIARWLDEAGTFMSGGPYRPGPGPGSGLSGYISNPPHTIRDDGGTP"
BASE COUNT         1298 a         2370 c         2305 g         1070 t
ORIGIN      
        1 ggtggccgcg tgttcggttc gcactaccac tacacgtgga tgaagaacct gatcccggcg
       61 tggacgccga ctgcctcgtg gggcggctcg tactcgacga tgacgcctga cctcgtcgac
      121 acctcgcatc ccgacggcat ggcgttctcg cagtggctgc aggctgctgg cgcgtcgacg
      181 gcgcccggcc aggtcgcgct cggcgtgaag acgtacgacg cgggcccggt caacgcgccg
      241 accacacgct ggctctacgc gtcgggcacg aacccgacga cccactacat gagcttcaag
      301 acgccggttg gcgttccgat ggagcagcag tgcggcaagg tcgtgtacgc cggcctgcac
      361 gtcagctcga cctcgtcggt cggttcggac ttccccgccg gctgcaccgg cacgttcacg
      421 ccggacgaga aggcgctggc cttcctgctg ttcgacctcg gctcctgcgt cgacgtgatc
      481 ttctaggcac accgtcacgc acaccgaaaa gccgggcctg gatcacaatt tccgggcgtt
      541 gaggcacgcc gcgctgcgcc acgcgcgacg taggccagat ctgcactcga cgaatgctca
      601 agacgcgcat atcgtcctgc ccgcgatgcg cggtctcacc caattcctcg tcgttggcct
      661 ttccatctct atgctcgcag cgtgcggacc gaacaaacgc ggcgaggatg acgatctgcc
      721 ggacgcgccg cctcccgacg caccgtgccc gacgacaatc agtggcaagg tgttcgcgcc
      781 gaacggcacg ctgccgctct acaacgtcac ggtctacatt ccgcagacgg cgcccggacc
      841 gttcaccgac ggcgtcagct gcacgcactg ctcgacggat cttccgggcg gtgttgtcac
      901 gaaggcgatc tcggacgccg agggcaagtt cacgctcgag aacgtgccgc ccggcaccga
      961 cgtcccggtc gtcatcacga ccggcaagtg gcgccgtcag ctgacggtgc cgtacgtcgc
     1021 cgagtgtgtc gacacgccga tccaggatgg cgtgttccgc ctgccgaaga accgcaccga
     1081 aggcgacctg ccgcgcatcg cgatggtcac cggtgcttgc gacgggctcg cgtgcatctt
     1141 ctcgaagatg ggcgtctcgg cgtcggagtt cggctcgagc tcgagcggtc ccgaacgcat
     1201 catttggtac aacgcgcacg gcggcgtgtc tccgggcact tcgcagcctg cgcagaccgg
     1261 gctgtggacg aacctcgccg agatgcagaa gttcgacatg gtgatcaact cgtgcgagtg
     1321 cagcgagtac caaacggaaa agactgcgcc cgatgtgatt cgtcagtacg ccgacatggg
     1381 cggccgcttc ttcggatcgc attaccagta cacgtggatg caggagaagg ttacgcagtg
     1441 gcagggcact gccgcgtaca tggacagcac cattggctct tccgtggtga gcgtcgacac
     1501 gtcgcatccc gacggcatgg cgctcgcgaa gtggctgagc gcggtgggtg gctcgacgac
     1561 gccaggccag ctgatgctca gcaccacatc gcagccgagc gagaccgcgg tcaactcgcc
     1621 gacgacgcgc tggctgtacg atcccagcta cgtctattac atgtcgttca agacgccggt
     1681 cggcgtgccg atggaacagc agtgcggcaa ggtcgtccac gccggcatcc acgtcagcga
     1741 gagctccagc gtcaacgcgt cgttcccgag cgggtgctcg acatcgctga cgccggacga
     1801 gaaggcgatg atcttcttga tgttcgatct cggctcctgc gtcgacgtga tcttctagcg
     1861 ctcgcgaatc tttgcgagaa acttctccgc ggtctcgctc atcacctcgg cgagcagacg
     1921 gaactcgcgc aggcgcgcgg atgacgtgcg ccatgcgaga ccgaccgtgc gcccgggcgg
     1981 cttcgtgaag tcggcgacct cgacgggtcc gcgcggctgg acgagcgcct cggcggcggc
     2041 ttccggaagc agcgtgacgc cgaggccgcc ggccaccatc tgcacgagcg tcggcaagct
     2101 cgtcgcgcgc acgccgatcg actcgaccgc gccgccgcgt tcgcacacgg cgagcgcctg
     2161 atcgcgcagg cagtggccgt cctcgaggag cagcacggtc tgatcctcga gatcgcgctc
     2221 gctgagcttg ccgtgcttcg cgagcacgtc ggcaccacgc ggcgccgcga gcatgaacgt
     2281 ctcgcgtgcg atcggtgcgg tcgtcacgtc gcctgggacc ggaatcgcga gcagcgcgag
     2341 gtcgagttgc cctgccgata gctgcgcgag caaacgcgcg gtctggtcct cgcgcaagat
     2401 cagctcgagc tttggaaagc gcttgcgcac gaccggcagc agcgcgggca accagtacgg
     2461 cgcgatcgtc ggaatcacgc cgaggcgcag cgggccaacg agtggctcgg cgcggcggcg
     2521 cgcagcgtcg ttgaccgcgt cgatcgattc gagcgcgatg cgcgagcggc cgacgacatc
     2581 ctcgccggcg ggcgtgacga gcacgctgcg gcgatcgcgt tcgaacacct gcacgccgag
     2641 caagtcctcg gcgctctgta cctgcgcaga cagcgcgggc tgcgagatac cgagcgaagc
     2701 ggctgctttg cgaaaacttc cgtgatctgc gacggcaacc aggcattcga gctgccgcgt
     2761 cgagggcctt tccatgatag gcgcactcta tcacaggcat cgcttttggc gattggacct
     2821 ttcagtgagg gaaccgcaga gtgtctccat caaggaggca acgagatgct tacgatcgga
     2881 gatcagttcc cagcgttcaa ggcgacggca gtagtgagcc aggagaaggg caaggagttt
     2941 ctcgagatca cggaccagac ttacgacggc aagtggaagg tctacttctt ctggccgaag
     3001 gacttcacgt tcgtgtgtcc gaccgagatc gcggcgttcg gcaagctgga acgcgacttc
     3061 gcggatcgcg aagcgcagct gctcggcgtg tcgatcgata gcgagtacgt ccacctcgcg
     3121 tggcgcggct cgcatcccga cctcaaggac ctgccctacc cgatggttgc cgacatcaag
     3181 cgcgagctat cgacggcgct cggcgtgctc gacaagcgtg aaggtgtggc gctgcgcgcg
     3241 acgttcatcg tcgatccgca gaacgtgatc cgcttcgtgt cggtcaacga tctcagcgtc
     3301 ggccgcaacc cggccgaagt gatgcgcgtg ctcgacgcgc tgcagactga cgagctctgc
     3361 ccgtgcaact ggcagaaggg tcagaacacg ctgaacgcgg cctaaggacg acgatgacga
     3421 acctcgacca attgaaagag cgcctaccgg actacgcgcg cgatctgcgg atcaacctcg
     3481 gcgtgatcgg ctcgtcgacc gcgctgtcgc cggtgcaggc gtggacgatc gcgctcacca
     3541 gcgcagcgac gacacgcaat cgcgacctcg tcgctgccgt cgaggctgac gcggccgcac
     3601 acgtcgagcc gactgcgttg accgcagcgc gcggtgcagc ggcgatcatg ggcatgaaca
     3661 acgtctacta ccggttcctg cacttcatgg gcgaagcgcc tccgtacggc cacatgcctg
     3721 cgcgtctgcg catgcaggtg atcggcaacc ccggtatcga caagctcgac ttcgagctcg
     3781 cgtgccttgc ggcgtcggcg atcgcgggtt gcgaggcctg cgtgcgctcg cacgagaagg
     3841 cagtacggga gcgcggcggt acgaaagagc aagtgcaaga cgctgtgcgc atcgctgcgg
     3901 tactcaacgg ggtcgcggtc gcgctcggct gatttcgcag ggcacgtcgc gtgcacgact
     3961 cgatctcaaa tcaaggagtt ggtcatgcag cctcacgccc cccgcgcagt tggcccgaag
     4021 acgacgaacg gccacaacaa accagaccgc acgctgagtc acggcagacc ggccgaaccg
     4081 cttctgcggg tccttccgat caagcgttgg atcccgcaag acgtgcactc gatgctggac
     4141 tacgcagcag gcgtcagcgt cgcgagcggc cacttcacca ctgatgacga ggccgcgcgc
     4201 tgggcaagtg tagccctcgg tgcgagcgtg atcggcgtgt cgctgctcac ggactaccgc
     4261 ctgagcctcg tcaagctgat cccgatccgc gcgcacgaag cactcgacta cgtgtggggc
     4321 gcgtcgtgca tcgcggcgcc gtttgtactc ggctactgga agaaatcgcc gctcaccgcg
     4381 atcacgcata tcgcggtcgg cgcgaccacg atcctagggt cgctagtcac cgactaccgc
     4441 tcgtaccaac aacagcgcga tgacgaacgg cgtcgttcgc aggctgacct ccagccgatc
     4501 atgccgacgt agatcgcgcg agccggatca cgtacgtgat cgccgcgacg ccgacggcga
     4561 gcgcgagtgc gaacacgacc gcgccaccag ccgttgacgc gagagcgtgc gtcggctcga
     4621 tcaacgcgac acgcggcgtc tcgcgtacga cgatcgccgc gacaatcgca ccggtgcccg
     4681 caccggtcac cagcgccagc gcacgctcgg tcccccgtcg gggatcacca acgctcggct
     4741 ggcgccacag ccacagccaa ccgccgagct cgacgacgag cgcgaccacg agcacgacca
     4801 gccaggcgcg tgcggcaccg tcgaccgcaa agccgctccg ccacagccac acggcgccgc
     4861 cgaccgacac gatgcgcgcg gcgatcgcga gcacggcgag gcgcttgcgt cctgcgtcgg
     4921 ccgcggacca cgctgcgatc atcgcgaacg tggtcgtcat cgccccaccg aagatcacga
     4981 agcgcggcgc gatcgattcg gcgaggaaca cccgcttgcc ggccgcgtag aacgcgtgcc
     5041 actcgggatc cgccttcatc agctcgtgca gctcgctcca cgaccatgcg acgaacgcga
     5101 agcagacgag gccgagcgcg agcgcgagct tgcgaaaccg ctccgacatc tttgcaaggt
     5161 agagcgcata gaagccgacg atcagcgccg gcacgacggc catccagcgc ggaccgagca
     5221 agaggtttgc agtgtagaag cgcgtctggt gcagaagctg gacgaacaac aggggtgcga
     5281 cgccggcggt gataccggcg ccgagcatga acggcagccg atcgcgcacg gtcgccgcca
     5341 ctggatcgtc gacacggcgt agtgactgga gcagcacgta cccggtgccg gcgacaacgt
     5401 agccgatgaa caccgcgtgg agcgcgagcg tcgcgacgta gagcgcgagc acaccgccgc
     5461 cgcgatcgaa cggccacatc atggcgtccc tccgtcgtcg cggatggtgt gcggtgggtt
     5521 tgagatgtag ccggagagtc cggatcctgg cccgggacca ggacggtagg gtccgccgct
     5581 catgaacgtc ccggcctcgt cgagccagcg cgcgatctgc gcgagcgtct cggggaccgc
     5641 gctggtcggc caggatgccg gagcaccctg catctcccag cgtatgagct gcacgatcgc
     5701 ttcgacatcc tccgcattgc ctgcgaacgg cggcatgaag ctcttcgtgt gctggagctt
     5761 cgccacgttc aaacgcagct ggtcatcggt ccacgttcgg ctcagctcga cgagcccgtt
     5821 cgcaccatcg atcgtgtggc aggcgtcgca gagcgtgcga tggaccttcg cgccgcgctt
     5881 cagctggtcg gtcggatagc gtgcttcgtc gcgcagcgga tatgggtcgc ccgcggtcgc
     5941 gccacgcacg cgcatagcag tgacatctgc tggcgcgatc gagctcgagt acaagacacc
     6001 acgcaccgtg tacggtttgc gcgcgccttc gcgaacgaac tctccggccg ccgtcgcccc
     6061 gaacgcgagc gcgagcagca gcgtcgcggt cgcgccattg atgtagagcc gctgcgctag
     6121 cagaccgatg agcgcatagc cgccgatcaa cagcgacgcg ccgcccgcga tgacgacgaa
     6181 catggtcatc gccatgctgc cgccgagcaa ccagctgcgg ctgtccggag gaatcacggc
     6241 gagaaaccag agcgcgagta gcggcatcgc agccatcggc gcggtgaacc gcgcggcacg
     6301 ccggatgagc gcggcacgcc gctcgcgatc gagatcgagc gtgttgatca cgatgcatgc
     6361 aacgagcgcc gccagcgtca tcgcgaccac cgtgcggaac agcagcgatg gccagaagct
     6421 cgggttgaag aaacctgccc acgttcggcc gctggtcgga tagccgagcg cgcccatcgc
     6481 gctatccacg atcgagccgc ccggagtgag ctgccaggac aggatgccgt tgatgaagaa
     6541 cagcgacagc caggcggcga cggcgtagat cgcgagcagc cggagccggg cgcgatcgcc
     6601 aagctggcgg ccgacgcgca cgaatgcata gcccgccgcg acctcgaccg agaagcacac
     6661 ccactcggtc gcccacagcc agtggaactg gtcgatcatg aggccgatcg tccgcgcgcc
     6721 gacctggatg gtcgtgaacc agatgccgac accggtcagc gccccgacga cgaacgagat
     6781 caggacgagg tactggaagt agccgtcgac gaacgtgcgt gcgtcacgtt ccttaccgcg
     6841 ctgcgccagc cgctcgaagt accagagcag catcccgcca ccgatcgcga actgggccag
     6901 aaatacgtgc agaatgccga ccccgccgat gaccatgccc ttcatcagcg gcccgaagtc
     6961 gttgaccgga tagaacggga tcggcgccac gccagtcgga tgctagcgcg ccgcctcccg
     7021 tggtgcgtcg aaatgccgcc act
//
LOCUS       DASUFX010000765         5836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1234518, whole genome shotgun sequence.
ACCESSION   DASUFX010000765 DASUFX010000000
VERSION     DASUFX010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234518"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFV99_30960"
     CDS             complement(<1..1135)
                     /locus_tag="VFV99_30960"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5063855.1"
                     /translation="MVRDILELGGSLGFLDEIYDQYQQQPDAVDASWHDVLNGQAAPA
                     EQAVETNGRGNGKGNGHAQVEARNKTGTTRAMPMFERPGAVTLAPITAKAGQPSIWPL
                     VNAYRSRGHFAANLDPLGLLETARIVELDPATWGFSEQDMDRVIEPTGVHGMPKATLR
                     DVLVHLKKIYAGSAGLEFMHISSPAKRSWLAERSETCLAKKLPTEVRTRMLALLINAE
                     QFERFCHTKYPGTKRFSLEGSESLIPALDLVLTHAARLGAIEAVLGMAHRGRLTTIEQ
                     ILKRPARDLFGHFEDVEPEKALGGGDVKYHLGFSTDRTDPNGYAMHVSLAFNPSHLEA
                     VDPVVVGRVRAKQTRHGDLEHRRVVGVLVHGDAAFAGQGLVPET"
     gene            complement(1156..1800)
                     /locus_tag="VFV99_30965"
     CDS             complement(1156..1800)
                     /locus_tag="VFV99_30965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063856.1"
                     /translation="MKLLVALAVLPGCFLFRSGDSASCPKDRTVELGLQDEVKKLAGC
                     TQVSRLVIRTGATLELSPLAELEEITGDLSIGPTVGLDEAAFNGLLHVGGTIRVANNG
                     SLRGLFFPRLERAGRIEIENNVVLNSISMPRLAAVDGAAVIADNGGLEMISAPLLVSV
                     GKELVISGHAKLNLLEMSHLATVEALRVENNPKLPPETVEQLTSKSALDKPPTP"
     gene            complement(1797..2555)
                     /locus_tag="VFV99_30970"
     CDS             complement(1797..2555)
                     /locus_tag="VFV99_30970"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5063857.1"
                     /translation="MAINLSVNLNKVALLRNSRGAQNPSPRVAGIACLENGAYGLTLH
                     WRQDNRHTRKDDVHVLRQLCKERNAEFNLEGDDREELIALAEELSVTQFTLVPVKPGE
                     ITSDHGWDLPRQHDVVAPIVQRLKAKGIRTAIFVDPNPDMMAAAAATGVDRIEIYTEP
                     YASAWGRPELKTELDRLKRTAQAAVKHGMKVNCGHDLNLRNTPLLAREVPEIDEASIG
                     HALIADALYLGFPEAVRRYARVCRGEAVDAPVTQ"
     gene            complement(2574..3260)
                     /locus_tag="VFV99_30975"
     CDS             complement(2574..3260)
                     /locus_tag="VFV99_30975"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5063858.1"
                     /translation="MATRRELLITGLGTTALIACRHGAAKAGGEREDEEEVTPAEDLM
                     REHGVLRRILIVWAEAVRRLQANESLPPDVLASSTAIIRTFIHDYHEHNEEQYLFPRF
                     EAAKREVPLIQTLRKQHEIGRALTDRIAAAANPAALGDPAKRAGVIASIADFTRMYRA
                     HAAREDTVLFPALAPLVGHKEYEELGEKFEEDEHKRFGEGGFEQVVTEVAAIEATLGI
                     ADLATFTPTL"
     gene            3452..4075
                     /locus_tag="VFV99_30980"
     CDS             3452..4075
                     /locus_tag="VFV99_30980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063859.1"
                     /translation="MTAKKDFKRRVRERQAKTGESYTAARAQVMAQAGSTDAAVLEST
                     AESKAESKPSAISVVELVDASEEARSLGLRCDVLVSPKLAAHIPPIEILEQVLEALLA
                     TENDPQLSLMRGALFRGESPPNHPPVNMRARWEDTKRFFTRAGVGIGGINEDGDMLAL
                     QVEDTMVLVQISAVPHMPPIRRAQLRVFLSTVDAFRLDGDRAALLPR"
     gene            complement(4072..5163)
                     /locus_tag="VFV99_30985"
     CDS             complement(4072..5163)
                     /locus_tag="VFV99_30985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063860.1"
                     /translation="MRKLLLLTLLTSSIAYADDPAPPAAKTVAPAPPAAKAVEPAAPA
                     AKSVVVHVAPTSTVAGKPIELEAMIDAPFAEQLSVRWRTIGASEWHDVPFERSSAGGW
                     FASLPAAASPGVEYYIRGADQTGGETDHFASAASPHVVRVDPTLSDRLESLDRERLAN
                     RLDEVSFDVVAHNYGNRYDIPDWFVRSEATYTHNLLRVLHQVGFGFGTIGGRTPTGRM
                     TTADDVWHSLNYGYGQLRMRLHPSVFLDGRVGLGVSNENFVGLGRGVITFGKPWRSNV
                     SVGGEYFGDLGPTAFVRLQWDTAWPVLMGASVVRCDLPGVIVDPAGLYIAYDVAYQME
                     RVTVKAQLSYGARDGSPHAGGGIGTAIAF"
     gene            complement(5169..>5836)
                     /locus_tag="VFV99_30990"
     CDS             complement(5169..>5836)
                     /locus_tag="VFV99_30990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063861.1"
                     /translation="YTLAELDFTYRFMSPVYAVRLGWGSLRGTGGNKDVIDNDPNNTC
                     RTEMGTGEYACQRMSFSYVYTELELRARPNVAVMIRPQAGVLVTEKDDMTGGTDRCSG
                     GSIDPNDPRCQFFTGFGVRGRVRLGQEMGTNLVLGAAFTSHVGTLLEAAYHWLPNKVV
                     PVQITVQVTDLPVVENFGVRLVGDVGVRKLSWFYPSARVSYQARDLDHSGFSGGFALN
                     FDW"
BASE COUNT          964 a         1937 c         1956 g          979 t
ORIGIN      
        1 gcgtctcggg cacgaggccc tggccggcga atgcggcatc gccgtggacg agcacaccga
       61 cgacgcggcg atgctcgagg tcaccatgcc gcgtttgctt cgcgcgcacg cgaccgacga
      121 cgaccggatc gacggcctcg aggtggctgg gattgaacgc gagcgacacg tgcatcgcgt
      181 agccgttcgg atcggtgcga tcggtggaga agccgaggtg atatttgacg tcgccgccac
      241 cgagcgcctt ctccggctcg acgtcttcga agtgaccgaa cagatcgcgc gcaggccgct
      301 tgaggatctg ctcgatcgtg gtgaggcggc cgcgatgcgc catgccgagc acggcctcga
      361 tcgcgccgag tcgcgccgcg tgcgtgagca cgagatcgag tgccgggatc aagctctcgc
      421 tgccttcgag cgagaaccgc ttcgtgcccg gatacttcgt gtgacagaaa cgctcgaact
      481 gctcggcatt gatgagcagc gcgagcatgc gcgtgcgcac ctcggtcggc agcttcttcg
      541 cgagacacgt ctcgctgcgc tcggcgagcc agctgcgctt ggccggcgac gagatgtgca
      601 tgaattcgag gcccgcagag cccgcgtaga tcttcttcaa gtggacgagc acgtcgcgca
      661 gtgtcgcctt cggcatgccg tgcacaccgg tcggctcgat cacgcgatcc atgtcttgct
      721 cggagaaccc ccacgtcgcg ggatcgagct cgacgatgcg cgcggtctcg agcaaaccga
      781 gcggatcgag gttcgccgcg aagtggccgc ggctgcggta cgcgttgacg agcggccaga
      841 tcgacggctg cccggccttc gcggtgatcg gcgctagcgt gaccgcaccc ggccgctcga
      901 acatcggcat cgcgcgcgtc gtgccggtct tgttgcgcgc ttcaacctgc gcgtgaccgt
      961 tgcctttgcc gttgccgcga ccgttcgtct cgactgcctg ctcggccggg gccgcctgcc
     1021 cgttcagcac gtcgtgccag ctcgcgtcta ccgcgtcggg ttgttgctga tactgatcgt
     1081 aaatctcgtc gagaaaaccg agcgaaccgc caagctcgag gatgtcgcga accatgccgc
     1141 gatgatagcg cccggctacg gggttggcgg cttgtcgagg gcggatttac tggtcagttg
     1201 ctcgacggtc tcgggcggca gcttcggatt gttttcgacg cgcagcgcct cgaccgtagc
     1261 caggtgcgac atctcgagca aattcagctt cgcgtgaccg gaaatcacga gctccttgcc
     1321 aacgctcacc agcagcggtg cactgatcat ctcgagcccg ccgttgtcgg caatcaccgc
     1381 agcgccgtcc accgcggcta gccgcggcat cgagatcgaa ttcagcacca cgttgttctc
     1441 gatctcgatg cggccggcgc gctcgaggcg cggaaagaac aagccgcgca gcgagccgtt
     1501 gttcgcgacg cgaatcgtgc cgccgacatg tagcaaaccg ttgaacgctg cctcgtcgag
     1561 gccgacggtc ggcccgatcg acaggtcgcc ggtgatttcc tcgagctcgg caagcggcga
     1621 cagctcgagc gtcgcgccgg tgcgaatcac gaggcgcgat acctgcgtgc aaccggcgag
     1681 cttcttgacc tcgtcctgca gcccgagctc gacggtgcga tccttcgggc aactcgcact
     1741 gtcgccgctg cgaaacagga agcagcccgg caagaccgcg agcgcgacca agagcttcat
     1801 tgcgtgaccg gcgcgtcgac agcctcgccc cgacacaccc gcgcatagcg acgaacagcc
     1861 tcgggaaacc cgaggtacag cgcgtcggcg atcagcgcgt gaccgatcga cgcctcgtcg
     1921 atctccggca cctcgcgcgc gagcagtggc gtgttgcgca ggttgagatc gtggccgcag
     1981 ttgaccttca tgccgtgctt gaccgcggcc tgcgcggtgc gcttcagtcg atcgagctcg
     2041 gtcttgagct cgggcctgcc ccacgccgat gcgtacggct cggtgtagat ctcgatgcga
     2101 tcgacgccgg tcgcggcagc cgcggccatc atgtcgggat tcggatcgac gaagatcgcg
     2161 gtgcggatgc ccttcgcctt gaggcgctga acgatcggcg cgacgacatc gtgctgacgt
     2221 ggtaagtccc agccgtggtc gctcgtgatc tcgccgggct tgaccggcac gagcgtgaac
     2281 tgcgtgacgc tgagctcctc ggcgagcgcg atcagctctt cgcgatcatc gccctcgagg
     2341 ttgaactccg cgttgcgctc cttgcacagc tggcggagca cgtggacgtc gtccttgcgc
     2401 gtgtggcggt tgtcctgccg ccagtgcagc gtgagaccgt acgcgccgtt ctcgaggcac
     2461 gcgatgccgg cgactcgcgg cgacggattc tgcgcgccgc gtgagttacg aagcagcgcg
     2521 actttgttga ggttgaccga caggttgatg gccacggcgg ggtcttacca ccgctatagc
     2581 gtcggtgtga acgtcgcgag gtcggcgatg ccgagggttg cctcgatcgc tgccacttcc
     2641 gtcacgacct gctcgaaccc gccctcgccg aaccgcttgt gctcgtcctc ctcgaacttc
     2701 tcgccgagct cctcgtactc cttgtgcccg acgagcggtg cgagcgcggg gaacaggacg
     2761 gtgtcctcgc gcgcggcatg cgcccggtac atgcgcgtga agtcggcgat cgaggcgatc
     2821 acacccgcac gctttgcagg atcgccaagc gcggccgggt ttgccgccgc cgcgatccga
     2881 tcggtgagcg cacgcccgat ctcgtgctgc ttgcgcagcg tctggatcag cgggacctcg
     2941 cgcttggccg cctcgaaccg cgggaacaga tactgctcct cgttgtgctc gtgatagtcg
     3001 tgaatgaatg tccggatgat cgcggtcgag ctcgcgagca cgtcgggtgg gagcgactcg
     3061 ttggcctgca gccggcgcac cgcctcggcc cacacgatga ggatgcggcg cagcacgccg
     3121 tgctcgcgca tcaagtcctc ggcaggagtt acctcctctt cgtcctcgcg ctcgccaccg
     3181 gccttcgccg cgccgtgccg acacgcgatc agtgccgtcg tgcctaaccc ggtgatcagc
     3241 agctcccgtc ttgtcgccat gccacggctt accactgcta tgcttcggtg gcgcgggtgc
     3301 tcacgcgtaa ctctcatcgg tcctcccatg ccggaccgcc ccgagagcaa agggaagccc
     3361 tcttctcgag cgcgtatcgc tcacatcgct gtcctcgcgt tttgcggggt gcatgggacc
     3421 acgcacgacg cgaagaggaa gcgagcgacc tgtgaccgca aagaaagact tcaagcgccg
     3481 tgtgcgcgag cgccaggcca agaccggcga gagctacacc gccgcgcgcg cccaggtgat
     3541 ggcgcaggcc ggcagcaccg acgccgccgt cctagaatcc acggccgagt cgaaggccga
     3601 gtcgaaaccg tcggcgatct cggtcgtcga actggtcgac gcgagcgaag aagcgcgctc
     3661 gctcggcctt cgctgtgacg tgctcgtatc gccgaagctg gcggcgcaca tcccgccgat
     3721 cgagatcctc gaacaggttc tcgaagcact gctcgcgacc gagaacgatc cgcagctgag
     3781 cctgatgcgt ggcgcactgt ttcgtggtga gtcgccgccg aaccacccgc ccgtcaacat
     3841 gcgcgcgcgc tgggaagaca cgaagcggtt cttcacccgc gccggcgttg gcatcggtgg
     3901 catcaacgag gacggcgaca tgctcgcgct gcaggtcgag gacacgatgg tgctcgtgca
     3961 gatcagcgcg gtgccacaca tgccgccgat ccggcgcgca cagctgcgcg tgttcttgtc
     4021 gaccgtcgat gcgtttcggc tcgacggcga ccgagcggcg ctgctcccgc gctagaacgc
     4081 gatcgcagtt cctatgccac caccggcgtg cggcgagcca tcgcgcgcgc catacgacag
     4141 ctgcgccttc accgtgacgc gctccatctg gtacgcgacg tcgtacgcga tgtagaggcc
     4201 ggctggatcg acgatcacgc cgggtagatc gcagcgcacg accgacgcgc ccatcaacac
     4261 gggccatgcc gtgtcccact gcagccgcac gaacgcggtc gggccgagat cgccgaagta
     4321 ctcaccgccg acggagacgt tcgaccgcca cggcttgccg aacgtgatca caccgcggcc
     4381 gaggccgacg aagttctcgt tgctgacgcc gagaccgacg cgaccgtcga ggaacaccga
     4441 cggatgcagc cgcatgcgca gctgaccgta gccgtagttc agcgagtgcc agacatcgtc
     4501 ggcggtggtc atccgaccgg tcggcgtgcg cccgccgatc gtgccaaagc cgaagccgac
     4561 ctggtgcagc acgcgcaaca ggttgtgcgt gtacgttgcc tcgctgcgca cgaaccagtc
     4621 ggggatgtcg tagcggttgc cgtagttgtg ggcgacgacg tcgaacgaca cctcgtcgag
     4681 gcggttcgcg aggcgctcgc gatcgagcga ctcgagccga tcggagagcg tcggatcgac
     4741 gcgcacgacg tgcggcgatg cagccgatgc gaagtggtcg gtctcgccac ccgtttgatc
     4801 ggcgccgcga atgtagtact cgactccggg cgacgccgca gcgggcaagc tcgcgaacca
     4861 gccgccggca ctcgagcgct cgaacggcac gtcatgccac tcggacgcgc cgatggtgcg
     4921 ccagcggacg ctcagctgct cggcgaacgg cgcatcgatc atcgcctcga gctcgatcgg
     4981 tttgccggcg acggtcgatg tcggtgcgac gtgcacgacg acggacttcg ctgcgggtgc
     5041 ggccggctcg acagccttgg ccgcgggtgg agccggagcc acggtcttcg ctgcgggtgg
     5101 ggccggatcg tcggcgtagg cgatcgacga cgtgagaagt gtgagcaaga ggagtttacg
     5161 catggtgctc accaatcgaa gttgagggca aagccacccg agaagcccga gtggtcgagg
     5221 tcgcgcgcct gatacgacac acgcgcggag ggatagaacc aggacagctt gcgaacgccg
     5281 acgtcaccga cgaggcgcac gccgaagttc tcgacgaccg gcaggtcggt gacctgaacg
     5341 gtgatctgca cgggaacgac cttgttcggc agccagtgat acgccgcttc gagcaacgta
     5401 ccgacgtggc tcgtgaacgc ggcgccgagc acgaggttcg tgcccatttc ctggccgagc
     5461 cggacccggc cgcgcacgcc gaagccggtg aagaactggc agcgcgggtc gttcggatcg
     5521 atgctgccgc ccgagcagcg atcggtgccg ccggtcatgt cgtccttctc ggtgacgagc
     5581 acacccgctt gcgggcggat catgacggcg acgttcggac gcgcgcggag ctcgagctcc
     5641 gtatagacgt acgagaagct catccgctgg cacgcgtact cgcccgtgcc catttcggtg
     5701 cggcaggtgt tgttcggatc gttgtcgatc acgtccttgt tgccgccggt gccgcgcagc
     5761 gagccccagc cgaggcgcac cgcgtagacc gggctcatga agcggtacgt gaagtcgagc
     5821 tcggcgagcg tgtact
//
LOCUS       DASUFX010000766         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_679431,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000766 DASUFX010000000
VERSION     DASUFX010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679431"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFV99_30995"
     CDS             complement(<1..1116)
                     /locus_tag="VFV99_30995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063862.1"
                     /translation="MRSGVRFAAAVLLVPALARADRPSGVCVDVNVDFQPAVEPMQIV
                     AWVEDAAGTYVDTVYITQKTGRYGLGNRPGRLDFNTGSERGDTFPYGRREQTFPVWAH
                     RHGVTFPLVKFQNGDENNLSHPFAQSSPEMPPPYCRPIQPGEVEFDSGTCASATFTDK
                     GTFSSDETSKYPPRSDLRRRPEDSPSVDMYREINPFDAVSRATPPAGMLATATWAAPQ
                     RVDYGNYVLFVETSKTYDFNATYNATTYPAPSNIPWAEYGQPWRGQPSIVYRVPFTVA
                     MTSTEATIDTYEGYGDPTGQSGTLNPPDATIETTTPGSGASRLQLVSSGGSMYRVRVR
                     STAEFDGTPPATVEPPTMTQITSSSATIAFIAPGDDGT"
     gene            1156..1935
                     /locus_tag="VFV99_31000"
     CDS             1156..1935
                     /locus_tag="VFV99_31000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5063863.1"
                     /translation="MARTPSAEYLILDIETIPDVERWKRPEVTQDTPEPRVPFPPTWA
                     HRIVVIGCLWLDHGYRLKRLGVVGEATGAAGTPDERERLLLEDFSRFVGRARPVLVTY
                     NGRSFDLPVIAMRALCHGVSLGWYYRERNVRYRYSEEGHLDLCDWLADHGATRSGSLD
                     AVARLIGLPGKVGVDGSQVEGLYAAGQLAEIQRYCLADVTQTALLFLRFRLLQGVLSA
                     DTYQKVTRELLDALTADGRVNTVLEATDRAHLIGPAPLPTP"
     gene            1943..2557
                     /locus_tag="VFV99_31005"
     CDS             1943..2557
                     /locus_tag="VFV99_31005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5063864.1"
                     /translation="MTTIATIDTLADARLGAAWFHRDVVTVARELVGCALVHRKRAGM
                     IVETEAYLGPDDLASHARFGPTARTSVMFGPGGVSYVYLCYGVHQMFNIVTGVEGEGQ
                     AVLIRAVAPLVGLGDDPKVGRGPGKVTQALKLDRSHDRKDLATGQLYVAAHEKPPEVA
                     SGPRVGVDYAGDWAKKPLRFWWPEHPAVSRAPRVRATRAARRRG"
     gene            complement(2529..>2951)
                     /locus_tag="VFV99_31010"
     CDS             complement(2529..>2951)
                     /locus_tag="VFV99_31010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063865.1"
                     /translation="NLEVRVQDHFENAPTSDVWLTSRVDAKRILRFLDDHDVELLGNG
                     HLELSVYVRAHKATLRLTEHKTVVWLAEARALEAEITKWLRELGVPQVDKLVTVRDGS
                     HFHYRPLKSRDRKKLGEELYRQRLRKVATVTHGDAPHA"
BASE COUNT          449 a          984 c         1013 g          505 t
ORIGIN      
        1 cgtgccgtca tcgcctggtg cgatgaatgc aatggttgcg ctgctgctcg tgatctgcgt
       61 catcgtcggt ggttcgaccg tcgccggtgg cgtgccgtcg aattcggcag tcgagcggac
      121 gcgcacgcgg tacatcgagc cgcccgacga cacgagctgc aagcgcgatg caccggagcc
      181 gggagtcgtg gtctcgatcg tcgcgtccgg cgggttcagc gtgccgctct ggccggttgg
      241 atcgccgtag ccctcgtacg tgtcgatcgt cgcttcggtc gaagtcatcg cgaccgtgaa
      301 cggcacgcga tacacgatcg atggctggcc gcgccacggc tggccgtact cggcccacgg
      361 aatgttcgag ggggcgggat acgtcgtcgc gttgtacgtc gcgttgaagt cgtacgtctt
      421 gctcgtctcg acgaacagca cgtagttgcc atagtcgacg cgctgcggcg ctgcccacgt
      481 cgcggtcgcg agcatgccgg ccggcggcgt cgcgcgtgac acggcgtcga atggattgat
      541 ctcgcgatac atgtccaccg acggcgagtc ctcgggacgc cgccgcagat ccgagcgcgg
      601 cggatacttc gacgtctcgt cgctcgagaa cgtgcccttg tcggtgaacg tcgccgacgc
      661 gcacgtgccg ctatcgaact ccacctcgcc gggctggatc ggccggcaat acggcggcgg
      721 catctccggc gagctctgcg cgaacggatg cgagaggttg ttctcgtcgc cattctggaa
      781 cttcacgagc ggaaacgtga cgccatggcg atgcgcccac acaggaaacg tctgctcgcg
      841 acggccgtac ggaaacgtgt cgccgcgctc ggagccggtg ttgaagtcga ggcgacctgg
      901 gcgattgccg aggccatacc ggccggtctt ctgcgtgatg tagacggtgt cgacataggt
      961 gccggccgcg tcctcgaccc aggcgacgat ctgcatcggc tcgacggcag gctggaaatc
     1021 gacgttgaca tcgacgcaca cgccgctcgg gcggtccgcg cgcgcgagtg ctggaaccag
     1081 caagacagct gctgcgaacc gcacgcccga gcgcatcact tgcagtgtgt cacaccttcg
     1141 gcctagcgtc ggcacgtggc acgtacgccc agcgccgaat atctgattct cgacatcgag
     1201 acaattcccg acgtcgagcg ctggaagcgt cccgaagtga cccaggacac gccggaaccg
     1261 cgtgttccgt tcccaccgac gtgggcgcat cgcattgtcg tgatcggctg cttgtggctc
     1321 gaccacggct accggctcaa gcggctcggc gtcgtcgggg aggcgaccgg cgcggccggc
     1381 acgcccgacg agcgcgagcg tctgctgctc gaggatttct cgcggttcgt cggccgcgcg
     1441 cggccggtgc tcgtcacgta caacgggcgc agcttcgatc taccagtcat tgccatgcgc
     1501 gcgctctgcc acggcgtctc gctcggttgg tactaccgcg agcgcaacgt tcgctatcgc
     1561 tactccgagg agggccacct cgatctgtgc gactggctcg ccgatcacgg cgccacgcgc
     1621 tcgggcagcc tcgacgctgt cgcgcgcctc atcgggttgc ccggcaaggt cggcgtcgac
     1681 ggctcgcagg tcgaaggcct ctacgccgcc ggccagctcg ccgagatcca gcgctactgc
     1741 ctcgccgacg tcacacagac cgcgctgctg ttcctgcgtt tccgcttgct gcagggcgtg
     1801 ctcagcgccg acacgtatca gaaggtgacg cgcgagctcc tcgacgcgct gaccgcggat
     1861 ggtcgggtca acaccgtgct cgaagcgacg gaccgcgcgc atctgatcgg gcctgcgccg
     1921 ctgccgactc catgatcgcg cgatgaccac gatcgccacg atcgatacgc tcgccgacgc
     1981 gcggctgggt gcagcgtggt ttcaccgcga cgtcgtcacc gtcgcgcgcg agctggtcgg
     2041 ctgcgcgctc gttcatcgca agcgcgcggg gatgatcgtc gagaccgagg cgtatctcgg
     2101 ccccgacgat ctcgcgagcc acgcccggtt cgggccaacc gcgcgcacgt cggtgatgtt
     2161 cggccccggc ggcgtcagct acgtgtacct ctgctatggc gttcatcaga tgttcaacat
     2221 cgtgactggc gtcgagggcg aaggtcaggc ggtgctgatc cgcgcggtcg cgccgctcgt
     2281 cgggctcggc gacgatccga aggtcggccg cgggcccggc aaggtcacgc aggcgttgaa
     2341 actcgatcgc agccacgacc gcaaggacct cgcaaccggc cagctctacg tcgcggcaca
     2401 cgagaagccg ccggaggtgg cgagcgggcc ccgcgtcggc gtcgactacg cgggcgactg
     2461 ggcgaagaag ccgctgcgct tctggtggcc ggaacacccg gcagtgtcgc gcgcgccgcg
     2521 agtccgagct acgcgtgcgg cgcgtcgccg tgggtgacgg tcgcgacttt gcgcaagcgc
     2581 tgacggtaca gctcctcgcc gagcttcttg cgatcgcggc tcttgagcgg gcgatagtga
     2641 aagtgcgagc cgtcgcgcac cgtgacgagc ttgtcgacct gcggaacgcc gagctcgcgt
     2701 agccatttcg tgatttcggc ctcgagcgcg cgcgcctcgg cgagccacac gacggtcttg
     2761 tgctcggtga gccgcagcgt cgccttgtgc gcgcgcacgt aaacggagag ctcgagatgg
     2821 ccgttgccga gcagctcgac gtcgtgatcg tcgaggaaac ggaggatccg cttcgcgtcg
     2881 acgcgcgagg tgagccagac gtcgctggtc ggtgcgttct cgaagtgatc ctgcacgcgc
     2941 acttcgaggt t
//
LOCUS       DASUFX010000767         8910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1234663, whole genome shotgun sequence.
ACCESSION   DASUFX010000767 DASUFX010000000
VERSION     DASUFX010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234663"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1445
                     /locus_tag="VFV99_31015"
     CDS             144..1445
                     /locus_tag="VFV99_31015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5063866.1"
                     /translation="MKIAVVGTGYVGLVTGAGFSEFGHTVTCVDIDNQRVEALRKGVI
                     PIHEPGLEELVRRNSSLGRLSFTTSTAEAVAGAQAVFIAVGTPSSHDGAADLQYVLAA
                     ARDIGRTIDGFTVVVTKSTVPVGTAEKVRKAIQGVTDRPFAVASNPEFLKEGDAVNDF
                     LKPARVVIGADDPRAVDTLRSLYQGMHRTNDRIMVMDIPSAELTKYAANAMLATRISF
                     MNELARLAEQVGADIERVRVGIGSDPRIGNKFLFAGCGFGGSCFPKDLRALIHTGREH
                     DVQMTIVDAVERANERQKRMLGRRVLNHFGGNLDGKKVAIWGLSFKPETDDVRESPAL
                     TLIEQLRDGGAKIAGYDPKAMPNIRALIGDAIELAPDPYAAATDADALVLVTEWHELR
                     SLDFEKLAQRMKSRALFDGRNVWSADEARKAGFVYYAIGRK"
     gene            1516..1950
                     /locus_tag="VFV99_31020"
     CDS             1516..1950
                     /locus_tag="VFV99_31020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063867.1"
                     /translation="MDADKVAGKVARVAVEVAGRAVGIRGAGNVMRLLEEPQSRGWDP
                     VVPADLHANPLPPVAEDGTAECWQCKTRLPFAQLDIAGNAYSCRPCMLRTAQANASHD
                     AAAIDVDTVKIGRGRWWLWPLGALALTGLIVAIFFVTKAHSL"
     gene            2013..2435
                     /locus_tag="VFV99_31025"
     CDS             2013..2435
                     /locus_tag="VFV99_31025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063868.1"
                     /translation="MGFFKDLKNKVTGGAATVRVNVSSAQRGRAVPVQVQATAKANGK
                     VSAVYLLVRATESAEFKANNEKVQGSKVSFETRITIAGAQEIKEGETYNWDGQLELPV
                     NTNPTLRGSIIDHTWEVQAGLDMPGNDPDSGWQTLDVT"
     gene            complement(2622..4100)
                     /locus_tag="VFV99_31030"
     CDS             complement(2622..4100)
                     /locus_tag="VFV99_31030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063869.1"
                     /translation="MPRLAFALPALFVTSVASAQSIEIDEFRPAIDSRGFLTLNGSQV
                     LDHMDMSFGLGSLQWGHRLLSFENGPATYSVDNMVSATLVAAFGIRAGIPLELGASLP
                     FTIMNGSRGPDQLGDPNNPNDDKLYRLDGQGLGDLGLHVKARIARAGHFGFGAIASVY
                     VPTSKSRDPFLGEAATTPQLVGIADATFGNLRLAVNGGIRVRRTTTFMDMGADGAPAT
                     MGSITTSTALPVGIAAAYGVVPEKVELVAEVFGAVPLGRSDGYQPLEALGGVKVYLAK
                     NSYLSLGAGRGLLPDRAGNPDFRAVIGIVFEPKPAQRAGARIPGDLVAEVPPKPADDL
                     PLDSDNDGIFDKDDKCPDVMENYNGIEDDDGCPERKHIIETKTEIVTLQPIEFEFDKD
                     VLRDSAYPILDEVVQALEDNPGITLVEVEGHTDEQGNDAYNLDLSKRRAATVMRYLVD
                     HGIDSLRLTSEGYGESRPVDRHHTQDAYKVNRRVAFSIKQRI"
     gene            complement(4179..6953)
                     /locus_tag="VFV99_31035"
     CDS             complement(4179..6953)
                     /locus_tag="VFV99_31035"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX5063870.1"
                     /translation="MRIALTSALVLFAACPAPSTPAPQAPQRPVVPVAVQPAAVKPAD
                     PAEMPKLRLPKTFAPSGYTATLAIDPARDGFDGVIAITGEVKEKTPVIWLHGKDLTIK
                     KASSTTGAITITPRGEDLLEVRGDFAPGALQLDIEYSGKLDTLNTAGAFKQTVNGTPY
                     VFTQFEALYARRVFPCVDEPDTKVPWQLTLDVPKKVIAVSNTPVVKESDVAAGHRYEF
                     AKTKPLPSYLIAFGIGPFDIVDAGKTKNGVPVRIITMKGRGAEAAYAAKNAGRVLELL
                     EDYFGMPYPYEKLDLMTIPIVLGFSAMENAGLVTFTEQLVLFDPKKPSWRQRQGFIGV
                     ASHELAHQWFGDYVTTAWWDDIWLNEGFATWLGRKIVARFEPAWHEELGELDTRMGAL
                     DADSVVTARKVRQPIEKLDDVYNAFDGITYVKGAAVLNTFEQYVGPDVFQKGIREYLK
                     ARAFGNATSENFVAAISAASGKDLAPAFATYLDQPGAPELETKLTCDKSGAKLDVTQQ
                     RFVPPGSPEPAAQPPWIMPVCVAYDRDGKRADTCTLITKQTDSIALEGKKCPRWVMPT
                     AHGRGYYRVRYTAPQATKLRDEAWPLLTWTERRAVFFDVVSATRYRPRGVRKPMPKTP
                     SAKLPLTLMLSLVPKLLAGGDRFTVGDALIVPAGMDRFVPDEQRAKYESWMRATFGPG
                     AAKLGLTPQDNDDVDAEANRTELVRAVAWFGRDPDLVKQDVDLAANWRDLPTATRGLV
                     LAIAVDASPDLHGKILRDVKTEQEHLRRDEMYAALAGVRDPKRVEAALELLLDPKVDI
                     RESQWMLLGASTEATRKVMERFVRVNKDKLLARLPNDSVTGMIGFVATLFSGSCDETV
                     RDETKRYVLANFGKLPGGERVVNQSFEAMDQCIASRKVLEPELRAFLSGVKIPKPAPA
                     KPAPAKPAKP"
     gene            6991..8487
                     /locus_tag="VFV99_31040"
     CDS             6991..8487
                     /locus_tag="VFV99_31040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063871.1"
                     /translation="MRALGLLVVVAACNPPGLTLEVVVDDPAMAKVELFAGRDCGECP
                     RATAPPGLQTMPVDAGYIVDDPEPFTVTKNEFSNGVAGFRIEADQDETLAVLVALAYD
                     AQGQIRWSWTGHGVQIPDGDSAHWRIQMSPTKPIDMNVGPLTNIERIAEWPHPKGHPS
                     CLLLEHWDDSSHARREMLSPADDFDCDGVDPTFECEPFIPNAMGVAPSIESANCVTPV
                     MPSGSSLYVCMLGGPQCNETPTMANDTCAELDTPYCTPISTCQCQNLPDAQACIRTLV
                     VGATSDQPTMPFMKCTIGVDQAGNRCDSEKLLIDASPFLGIAQGCKDLRIGDATTPIG
                     PFANALHIGDGAKLRLDNFIMPCAAEVSFEGTQIAPHVEYGFFDVALDNGYHLLTPAK
                     VDVHLGCNLPSICGVVMSNNTNESMFQCVGAMSVTPACAPDQAHGCNGPYCNGVCCGY
                     NERCTANGCSCGGGAGCTGTNTCAVGAANEDNCGSICCGPPPGPACPF"
     gene            complement(8484..>8910)
                     /locus_tag="VFV99_31045"
     CDS             complement(8484..>8910)
                     /locus_tag="VFV99_31045"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5063872.1"
                     /translation="EARAWVGLGAVAFRLFAIEWTLLPARDVGTYGFAGGNALCEVPE
                     GYRAVPHFDHQHHTRRYLALARKLEDEARDWFTEAKLDEIAAKFEAAEQVGRAQRPLT
                     FRSPKEMARIRPKKPGRNDLCVCGSMRKWKQCCGALLAD"
BASE COUNT         1555 a         2885 c         2918 g         1552 t
ORIGIN      
        1 gaaatgcatg gccgcggcga caggcgcggt cctcggtgcg gcatttgcgc tcgtcgcgag
       61 cgcgacgatc acgaaccaag cgtgcctcac agccaaccct catctcacgt aggagggcct
      121 cgtgcttgca acgcagcacg cgcatgaaga tcgctgttgt tggaaccggc tatgtcgggc
      181 tcgtcacggg agcgggcttc tcggagttcg gccacacggt cacgtgcgta gatatcgata
      241 accaacgcgt cgaagccttg cgcaaaggcg tcatcccgat ccacgagcca ggtctggagg
      301 agctggtgag gcgcaatagc tcactgggtc ggctatcttt cacgacgtcg acggccgaag
      361 cggtcgccgg cgcacaggca gtcttcatcg cggtcggcac gccgtcgtcc catgatggcg
      421 cggcagattt gcaatacgtg ctcgcggccg cacgcgacat cgggcgcacg atcgacggct
      481 tcacggtggt ggtcaccaag agcactgtgc ccgtgggcac ggcagagaag gttcgcaagg
      541 cgattcaagg cgtaacggac cgaccgttcg cggtcgcatc gaaccccgag ttcctcaaag
      601 aaggcgacgc ggtcaacgac ttcctcaagc ccgcgcgcgt cgtgatcggc gccgacgatc
      661 cgcgcgccgt cgacacgctg cgcagcctct atcaaggcat gcatcgcacg aacgatcgca
      721 tcatggtcat ggacatcccg tcggcagagc tgacgaagta cgctgcgaac gcgatgctcg
      781 cgacccgcat ctcgttcatg aacgagctcg cgcggctcgc cgaacaggtc ggcgcggata
      841 tcgagagggt gcgcgtcggc atcggctcgg acccgcgcat cggcaacaag ttcttgttcg
      901 cgggctgcgg cttcggcggt tcgtgcttcc cgaaggacct gcgcgcgctg atccataccg
      961 gtcgcgagca cgacgtgcag atgacgatcg tcgacgctgt cgagcgcgcg aacgaacgtc
     1021 agaagcgcat gctcggccgc cgcgtgctca accacttcgg cggcaacctc gatggcaaga
     1081 aggtcgcgat ctggggcctc tcgttcaagc cggaaaccga cgacgtccgc gagtctccgg
     1141 cgctcacgct catcgagcag ctgcgcgatg gcggcgcgaa aatcgccggc tacgatccca
     1201 aagcgatgcc caacatccgc gcgttgatcg gcgatgcaat cgagctcgcg cccgatccgt
     1261 atgcggcggc caccgatgcc gatgcactgg tcctcgtcac cgagtggcac gagctgcgtt
     1321 cgctcgactt cgagaagctc gcgcagcgga tgaaatcgcg cgcgctgttt gatggccgca
     1381 acgtatggtc cgccgacgaa gcgcgcaaag cgggcttcgt ctactacgcc atcggccgca
     1441 agtaggtcgg cgcgatctgc ctgcttagca gtctttgctt gaccgctcgc accgccgcgc
     1501 gataccaggc cgcgcatgga cgcggacaaa gttgccggca aggttgcgag agtcgctgtc
     1561 gaggttgctg gccgagccgt tggcattcgc ggggcgggca acgtgatgcg cctgctcgaa
     1621 gagccgcaat cgcgcggctg ggatccggtc gtgccggcag acctgcacgc gaacccactc
     1681 ccgcccgtcg ccgaagacgg caccgcggaa tgctggcagt gcaagacgcg gctgccgttc
     1741 gcgcagctcg acatcgcggg caacgcgtac tcgtgccgcc cgtgcatgtt gcgaacggcg
     1801 caggcgaatg cctcgcacga tgcagcggcg atcgacgtcg acaccgtcaa gatcggccgt
     1861 ggccggtggt ggctctggcc gctcggcgcg ctcgccctga ctggacttat cgtcgcgatc
     1921 ttcttcgtga cgaaggcgca ttcgctctag cgcgcagatt gtctgcgact gagcccaggt
     1981 cgctggctcg tcctttacgg aggacatcag caatgggctt tttcaaagac ttgaagaaca
     2041 aagtaacggg tggagcggcg acggtgcgcg tgaacgtctc gtcggcgcag cgcggccgtg
     2101 cggtgccggt acaggtgcaa gcgacggcga aggcgaacgg caaggtcagc gcggtgtact
     2161 tgctcgtgcg cgcgaccgag agcgcggagt tcaaggcgaa caacgagaag gtccagggct
     2221 cgaaggtgag cttcgagacg cgcatcacga tcgccggcgc gcaggaaatc aaagagggcg
     2281 agacctacaa ctgggacggt cagctcgagc tgccggtgaa cacgaacccg acgcttcgcg
     2341 gctcgatcat tgaccacacc tgggaagtcc aggcgggcct cgacatgccg ggcaacgatc
     2401 ctgactcggg ctggcagact ctcgacgtga cctgatcgaa aggctcagac ctcagacttc
     2461 agacctcaga cttcaggcct cggtgggaca gcagactcca ggctccgtgc tcgagtgatg
     2521 gacgcttcgt gcacggtcga tctctggcaa tcccgccgga tggcccgatg tctgacgcct
     2581 tgccgaggcc tgaggcccga gggcctgaag cctgtggcga cctagatccg ctgcttgatc
     2641 gagaacgcga cgcgacgatt caccttgtac gcgtcctgcg tgtggtggcg atcgactggc
     2701 ctgctctcgc cgtagccctc ggaggtgagg cgcaggctgt cgatgccgtg atcgaccagg
     2761 taacgcatca cggtggcggc gcgacgcttg gagagatcca ggttgtacgc gtcgttgccc
     2821 tgttcgtcgg tgtggccctc gacctcgacg agcgtgatgc cgggattgtc ttcgagcgcc
     2881 tgcacgacct cgtcgaggat tggatacgcg ctgtcacgga gcacgtcctt gtcgaactcg
     2941 aactcgatcg gctggagcgt cacgatctcg gtcttggtct cgatgatgtg cttgcgctca
     3001 gggcaaccgt cgtcatcctc gatgccgttg tagttctcca tcacgtcggg acacttgtcg
     3061 tccttgtcga agatgccgtc gttgtcggag tcgagcggca gatcgtcggc aggcttcggc
     3121 gggacctccg cgacgaggtc gccggggatg cgtgcgccgg cgcgctgcgc aggcttcggc
     3181 tcgaacacga tgccgatcac ggcgcggaaa tcgggattgc cggcgcgatc gggaagcaga
     3241 ccgcgacctg cgccgagcga caggtacgag ttcttcgcga ggtagacctt cacgccgccg
     3301 agcgcttcga gtggttggta gccgtcgctc ctgccgagcg gcaccgcgcc gaacacttcg
     3361 gcgacgagct cgaccttctc gggaacgacg ccgtacgcgg ccgcgatacc gacgggcaac
     3421 gcggtcgatg tcgtgatcga gcccatcgtc gccggcgcgc catcggcacc catgtccatg
     3481 aacgtggtcg tacggcgcac gcgaatgccg ccgttgacgg cgaggcgcag gttgccgaac
     3541 gtcgcatctg cgatgccgac gagctgcggg gtcgtcgcgg cttcgccgag gaatggatcg
     3601 cgcgacttgc tcgtcggcac gtacacgctc gcgatcgcgc cgaatccgaa gtgacccgcg
     3661 cgtgcgatgc gcgctttgac atgaaggccg agatcaccga ggccctggcc gtcgaggcga
     3721 tagagcttgt cgtcgttcgg gttgttcgga tcgccgagct gatcgggacc gcgcgagcca
     3781 ttcatgatcg tgaatggcag cgacgcgccg agctcgagcg gaatgccagc gcggatacca
     3841 aacgcagcaa ccagcgtcgc cgacaccatg ttgtcgaccg agtacgtcgc cggcccgttc
     3901 tcgaacgaca gcaggcgatg tccccactgc agcgagccga ggccgaacga catatccatg
     3961 tggtcgagaa cctgcgatcc gttgagcgtg agaaagccgc gcgaatcgat cgcaggtcga
     4021 aactcgtcga tctcgatcga ttgcgcagac gcaacagagg tgacgaagag cgcaggtaga
     4081 gcgaaagcca ggcgtggcat agcggtcctc ctcgacgcag cggagaggca gcgtcgactg
     4141 agaacactgt gcgcggccgg gacgcgcggg ttacatcgtc acggtttcgc gggcttggca
     4201 ggcgcgggct tggcaggcgc gggcttggga atcttcacgc ccgacaagaa cgcgcgcagc
     4261 tcgggctcga gcaccttgcg gctcgcgatg cactgatcca tcgcctcgaa gctctgattg
     4321 acgacacgct cgccgcccgg aagcttgccg aagttcgcga gcacgtagcg cttcgtttcg
     4381 tcgcgcacgg tctcgtcgca gctgcccgag aacagcgtcg cgacgaaccc gatcatgccg
     4441 gtcaccgagt cgttcggcag acgcgcgagt aacttgtcct tgttgacgcg cacgaagcgc
     4501 tccatcacct tgcgcgttgc ctcggtcgac gcaccgagca gcatccattg cgactcgcga
     4561 atgtcgacct tcggatcgag cagcagctcg agcgccgctt cgacgcgctt cggatcgcga
     4621 acaccggcga gcgcagcgta catctcgtcg cggcggaggt gctcttgctc ggttttcacg
     4681 tcgcgcagga tcttgccgtg cagatcgggg ctcgcatcga cggcgatcgc gagcacgagg
     4741 ccgcgcgtcg cagtcggcaa gtcgcgccag ttcgcggcga ggtcgacgtc ctgcttcacg
     4801 agatccggat cgcgaccgaa ccacgcgacc gcgcgtacga gctcggtgcg attcgcctcg
     4861 gcgtcgacgt cgtcgttgtc ttgcggcgtg aggccgagct tcgcagcacc gggaccgaac
     4921 gtcgcgcgca tccaggattc atacttcgcg cgctgctcgt cgggaacaaa ccgatccatg
     4981 ccggccggca cgatcaacgc gtcgccgacc gtgaaccgat cgccgccagc gagcagcttc
     5041 ggcacgagcg atagcatgag cgtcaacggc agcttggccg acggtgtctt aggcatcggc
     5101 ttgcggacgc cccgtggccg gtagcgcgtc gccgacacga cgtcgaagaa caccgcccgc
     5161 cgctccgtcc acgtcagcaa tggccacgct tcgtcgcgca gcttcgtcgc ctgcggtgcg
     5221 gtgtagcgaa cgcggtagta gccacggccg tgcgcggtcg gcatcaccca gcgcggacac
     5281 ttcttgcctt cgagcgcgat ggaatcggtc tgcttcgtga tcagcgtgca ggtgtcggcg
     5341 cgcttgccat cgcggtcgta cgcgacgcac accggcatga tccacggcgg ctgcgcggcc
     5401 ggctccggcg aacccggcgg cacgaaccgc tgctgcgtca cgtcgagctt cgcgccgctc
     5461 ttgtcacagg tgagcttcgt ctcgagctcc ggcgcacccg gttggtcgag gtacgtcgca
     5521 aacgcaggcg cgagatcttt gcccgacgcc gcactgatcg cggcgacgaa gttctcgctc
     5581 gtcgcgttgc cgaacgcgcg cgccttcaag tactcgcgga tgcctttctg gaacacgtcg
     5641 gggccgacgt actgctcgaa cgtattgagc acagccgcgc ctttgacgta cgtgatgcca
     5701 tcgaacgcgt tgtagacatc gtcgagcttc tcgatcggct gacgaacctt gcgcgcggtg
     5761 accacgctgt cggcgtcgag cgcacccatg cgcgtgtcga gctcaccgag ctcctcgtgc
     5821 cacgccggct cgaagcgcgc gacgatcttg cgcccgagcc aggtcgcgaa gccttcgttg
     5881 agccagatgt catcccacca cgcggtcgtc acgtagtcgc cgaaccactg gtgcgcgagc
     5941 tcgtgcgacg ccacgccgat gaagccctgg cgctgccgcc acgacggctt cttcggatcg
     6001 aacagcacga gctgctcggt aaacgtgacg agcccggcat tctccatcgc cgagaacccg
     6061 agcacgatcg ggatcgtcat cagatccagc ttctcgtacg gatatggcat gccgaagtag
     6121 tcctcgagga gctcgagcac gcgcccggcg ttcttcgcgg cgtacgcggc ttcggcgcca
     6181 cgtcccttca tggtgatgat gcgcaccggc acgccgttct tcgtcttgcc ggcgtcgacg
     6241 atgtcgaatg ggccgattcc gaacgcgatg agatagctcg gcagcggttt cgtcttggcg
     6301 aattcgtaac gatgaccggc ggcgacatcg ctctccttca cgaccggcgt attgctcacc
     6361 gcgatcactt tcttcggcac gtcgagcgtc agctgccacg gcaccttcgt gtcgggctcg
     6421 tcgacgcacg gaaacacgcg gcgcgcgtag agcgcttcga attgcgtgaa cacgtacggc
     6481 gtgccgttga ccgtttgctt gaacgcgccc gcagtgttca gcgtgtcgag cttgccgctg
     6541 tactcgatat cgagctgcag cgcaccgggt gcgaagtcgc cgcgcacttc gagcaggtct
     6601 tcgccgcgcg gcgtgatggt gatcgcgccg gtcgtgctcg acgccttctt gatcgtgagg
     6661 tccttgccgt gcagccagat caccggcgtc ttctctttga cctcgcccgt gatggcgatc
     6721 accccgtcga agccatcgcg cgccggatcg atcgcgagcg tcgcggtgta ccccgacggt
     6781 gcaaatgtct tcggcagccg caatttcggc atttccgcag gatccgcggg tttcaccgca
     6841 gccggctgga cggcgaccgg aacgaccggg cgctgcggcg cctgcggtgc aggcgtcgac
     6901 ggtgcggggc aagcagcaaa caacacaagc gccgacgtga gagctatgcg cacgtcgcgc
     6961 accatacagc ggatcgccta tgatcggcag gtgcgagcgt tggggctgct agtggtcgtc
     7021 gctgcgtgca atccgcccgg attgacgctc gaggtcgtcg tcgatgaccc ggcgatggcg
     7081 aaggtcgaat tattcgcagg ccgcgactgc ggtgagtgcc cgcgggcgac cgcacctccg
     7141 ggcttgcaga cgatgccggt cgatgccggt tacatcgtgg atgatccgga gccgttcacg
     7201 gtcaccaaga acgagttctc gaacggcgtc gccggatttc gcatcgaggc cgatcaggac
     7261 gagacgctcg cggtcctcgt cgcgctcgcg tacgacgcgc aaggccagat ccgatggtcg
     7321 tggaccggtc atggcgtgca gattcccgac ggcgattccg cgcactggcg gatccagatg
     7381 tcaccgacga agccaatcga catgaacgtc ggtccgctca cgaacatcga gcgcatcgcc
     7441 gagtggccgc atccgaaagg tcatccgtcg tgtctgttgc tcgagcactg ggacgacagc
     7501 tcgcacgcga gacgcgagat gttgtcgccc gccgacgatt tcgattgcga tggcgtcgac
     7561 ccgacgttcg agtgtgagcc gttcatcccg aacgcgatgg gcgtcgcgcc gtcgatcgag
     7621 tcggcgaact gcgtgacgcc ggtgatgcca agtggcagca gcctctacgt ctgcatgctc
     7681 ggtggcccgc agtgcaacga gacgccgacg atggcgaacg atacatgtgc ggagctcgat
     7741 actccgtatt gcacgccgat ctcgacgtgt cagtgccaga accttccgga cgcgcaggcg
     7801 tgtattcgca cgcttgtcgt cggcgcgacg agcgaccagc cgacgatgcc gttcatgaaa
     7861 tgcacgatcg gcgtcgacca agcgggcaac cgctgtgaca gcgagaagct gttgatcgat
     7921 gcgagcccgt tcctcggcat cgcgcagggc tgcaaggacc tgcgcatcgg cgatgcaacg
     7981 acgccgatcg gtccgttcgc caatgcgctg cacatcggcg acggtgcgaa gttgaggctc
     8041 gacaacttca tcatgccgtg cgcggccgag gtttcgttcg aaggcacgca gatcgcaccg
     8101 catgtcgagt acggcttctt cgacgtcgcg ctcgacaatg gctatcacct cctcacgccc
     8161 gcgaaggtcg acgttcacct cggctgcaac ctcccgtcga tctgcggcgt cgtcatgagc
     8221 aacaacacga acgagtcgat gtttcagtgc gtcggcgcga tgtcggtcac gccggcctgt
     8281 gcacccgatc aagcacacgg ctgcaacggc ccgtactgca acggcgtgtg ctgcgggtac
     8341 aacgagcgct gcactgcgaa tggctgtagc tgcggaggcg gcgctggctg caccggcacc
     8401 aacacctgcg cggtcggcgc ggccaacgag gataactgcg gcagcatctg ctgcggtccg
     8461 ccgcccggac ctgcgtgccc gttctaatcg gctagcagtg cgccgcagca ctgcttccac
     8521 ttgcgcatcg agccgcagac gcacagatcg ttacggcccg gcttcttcgg gcgaatgcgc
     8581 gccatctctt tcggcgaccg aaacgtcaat ggccgctgcg cgcgtccgac ttgctcggcg
     8641 gcttcgaact tcgccgcgat ctcgtcgagc tttgcttcgg tgaaccagtc gcgcgcttcg
     8701 tcctcgagct tgcgtgcgag cgcgaggtag cggcgcgtgt gatgctggtg atcgaagtgc
     8761 ggcaccgcgc gataaccttc gggcacctcg cagagcgcat tgccgccggc aaatccgtac
     8821 gtgccgacgt cacgcgcggg caacagcgtc cactcgatcg cgaacaagcg gaatgcgact
     8881 gcaccaagac cgacccacgc gcgcgcttcg
//
LOCUS       DASUFX010000768         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_818542,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000768 DASUFX010000000
VERSION     DASUFX010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818542"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VFV99_31050"
     CDS             complement(<1..1156)
                     /locus_tag="VFV99_31050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5063873.1"
                     /translation="MQVVDTTIVNVALPHMQGSMAATQDQISWVLTSYIVSAAILTPL
                     TGVLADRFGRKRLFAASVIGFTIASMLCGAATSLEQMVAFRALQGALGASLVPISQAV
                     LLDTYPREKHGSAMALWGMGVMVGPILGPTLGGYLTEYYSWRWAFYINLPVGVLAVLG
                     IAAFVPEVKGQAKRGFDFFGFALLSIAIGALQLMLDRGNTLDWFDSTEIVLEAATAGL
                     AFYLFLAHMFTADKPFIEPGLFADRNFVAGILLMFTVGVMLMSTMALLPPFLQSLLGF
                     PVLTAGFVLAPRGAGTMVAMMIVGRLIGKVDTRLLVLTGLGLMTWSLHAMTQWNADVG
                     VGEIVYTGIVQGIGLGFIFVPLSTIAFSTLAARYRNEGTAMFSLIRNIGAS"
     gene            complement(1253..2398)
                     /locus_tag="VFV99_31055"
     CDS             complement(1253..2398)
                     /locus_tag="VFV99_31055"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5063874.1"
                     /translation="MAEQDSPNISAPAPNAAPAAPAAPAARAPRRPLRSILLLAGPIV
                     ALVVGAYVYYTSGRVVGTDNAYVKADVGVISAEVAGPIATLNVRENQRVEAGDVLFTI
                     DDRPFRVALDRGNAQLAAINDFVESTKASYRQSLEQLALARTNAAYEQRELDRLTALA
                     ERKLTSDVAVDEQRHERDVANQEIQVTERALDAIRARLGGDLDRPVTEQAAYLAAKSM
                     RDAAELDLEHTVVRAPFSGIASQVPTLGHHVQPGSPIMTIVADRDMWIEANFKETDLT
                     HVAVGQPVDIRLDTYPDRRWRGSVASISQATGAEFSVIPAQNATGNWVKVAQRIPVRI
                     AIEMRGDDPELRAGMSAIVDIDTGHERPAPHWIRALLPSNSAFAESR"
BASE COUNT          420 a          806 c          856 g          401 t
ORIGIN      
        1 cgctggcgcc gatgttgcgg atcaagctga acatcgcggt cccttcgttg cggtagcgcg
       61 cggctagcgt cgaaaacgcg atcgtcgaca gcggcacgaa gatgaagccg agcccgatgc
      121 cttgcacgat gcccgtgtag acgatctcgc cgacgcccac gtcggcgttc cactgcgtca
      181 tcgcgtgcag cgaccaggtc atcagcccga gacccgtgag cacgagcagc cgagtgtcga
      241 ctttgccgat tagcctcccg acgatcatca tcgcgaccat cgtgcccgcg ccgcgcggcg
      301 ccagcacgaa gccggcggtc agcaccggga aaccgagcag gctctgcagg aacggcggca
      361 gcagcgccat cgtcgacatc agcatcacgc cgaccgtgaa catcagcagg atgcccgcga
      421 cgaaattgcg gtcggcgaac aggccgggct cgatgaacgg cttgtcggcg gtgaacatgt
      481 gggccaggaa tagatagaaa gcgaggcccg ccgtagcggc ttcgagcacg atctccgtcg
      541 agtcgaacca atcgagcgtg ttgccgcggt cgagcatgag ctgcagcgcg ccgatcgcga
      601 tcgaaagcag cgcgaagccg aagaaatcga agccgcgctt cgcttggccc ttcacctccg
      661 ggacgaacgc ggcgatgccg agcacggcaa gcacgccgac gggcaggttg atgtagaacg
      721 cccagcgcca gctgtagtac tcggtgagat agccgccgag cgtcgggccc agaatcgggc
      781 cgaccatgac gcccatgccc caaagcgcca tcgcggagcc atgcttctca cgcggatagg
      841 tatcaagcag caccgcctgc gagatcggca cgaggctcgc gccgagggcg ccctgcagcg
      901 cacggaacgc gaccatttgc tcgagcgacg tcgccgcgcc gcacagcatc gaggcgatcg
      961 tgaagccgat tacggacgcc gcgaacaagc gcttgcgtcc gaagcggtcc gcgagcaccc
     1021 ccgtcagcgg agtgaggatc gcagcggaca cgatgtacga ggtgagcacc cacgagatct
     1081 gatcttgcgt ggccgccatg ctgccttgca tgtgcggcaa cgcgacgttg acgatggtcg
     1141 tgtcgactac ctgcatgacg gtcgcgagca tgaccgacaa cgtgatcatg ccgcggttca
     1201 gcggcgctgc agcgctcgcc gtcgcgttga tcgccgtcgc gctcatgacc ggctagcggc
     1261 tctctgcgaa cgcggagttg ctgggcagca gtgcgcggat ccaatgcggc gcgggccgct
     1321 cgtgacctgt gtcgatgtcg acgatcgcgc tcatgccggc gcgcaactcc ggatcgtcgc
     1381 cgcgcatctc gattgcgatg cgcacgggaa tccgttgcgc gaccttgacc caattgccgg
     1441 tcgcgttctg cgccggaatc accgagaact cggcgcccgt cgcctggctg atgctcgcga
     1501 cgctgccgcg ccaccggcga tcggggtagg tgtcgaggcg tatgtcgacg ggctggccga
     1561 cggcgacgtg cgtcaagtcg gtctctttga agttcgcctc gatccacatg tcgcgatcgg
     1621 cgacgatcgt catgatcggc gatccgggct ggacgtgatg gccgagcgtc ggcacttggc
     1681 tcgcgatgcc gctgaacggc gcgcgcacga ccgtgtgctc gagatcgagc tcggcggcgt
     1741 cgcgcatcga ctttgcggcg aggtacgccg cttgctcggt cacgggccga tcgagatcgc
     1801 cgccgagacg cgcgcggatg gcatcgagcg cgcgctccgt gacctgaatc tcttggttgg
     1861 cgacgtcgcg ttcatggcgt tgctcgtcga cggccacatc ggacgtgagc ttgcgctccg
     1921 caagcgccgt gagccggtcg agctcacgct gctcgtaggc cgcgttcgtg cgagcgagcg
     1981 cgagctgctc gagcgattgc cggtacgacg ccttggtgct ctcgacgaag tcgttgatcg
     2041 ccgcgagctg cgcattgccg cgatcgagcg cgacgcggaa ggggcggtcg tcgatcgtga
     2101 acagcacgtc gccggcctcc acgcgctggt tctcgcgcac gttgagcgtt gcgatcggcc
     2161 ccgccacctc cgcgctgatg acgccgacgt cggccttgac gtacgcgttg tcggttccga
     2221 cgactcgtcc cgacgtgtaa tagacgtagg cgccgaccac gagcgcgacg atgggccctg
     2281 caagcagcag gatcgagcgc agcgggcgac gcggagcgcg tgccgcgggt gcagccgggg
     2341 cggcaggggc cgcgttaggc gcgggcgcgg agatgtttgg cgagtcttgt tcggccatga
     2401 cgagtgcttc ccgattggtc cggctgcgcc gccgtgactt cggcggcgag caggttgcgt
     2461 ttcatttgct cgagcgcggc aac
//
LOCUS       DASUFX010000769         6067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_958531,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000769 DASUFX010000000
VERSION     DASUFX010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958531"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..822
                     /locus_tag="VFV99_31060"
     CDS             67..822
                     /locus_tag="VFV99_31060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5063875.1"
                     /translation="MVRVERSGPVTTVILDRAAAKNAVDRDTAQALADAFRNFDADSS
                     ARVGVLWGDHGTFCAGADLKALAAGTPNRVAPDGDGPMGPTRMLLGKPVIAAISGHAV
                     AGGLELALWCDLRVAEDNATLGVFCRRFGVPLIDGGTIRLPRLIGLSRALDLILTGRA
                     VKADEALMMGLVNRVVPPGQARAAAEQLAHELAELPQAAMRGDRMSAYLQHDLDLEAA
                     LQKELELGSRALGEAVQGATRFTGGAGRHGGRE"
     gene            819..1106
                     /locus_tag="VFV99_31065"
     CDS             819..1106
                     /locus_tag="VFV99_31065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063876.1"
                     /translation="MIRGLFAASIIILLTSCIVRHLVSGPRLTGTCHGACDHYIECKP
                     GHEDLDRQRCETECPGVFSDRDSLMAYESLSCKNAVEYVDGTQPRSANTAR"
     gene            complement(1103..2383)
                     /locus_tag="VFV99_31070"
     CDS             complement(1103..2383)
                     /locus_tag="VFV99_31070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5063877.1"
                     /translation="MRTWLLVLAACSAPVIREPIEWSPPIEIAHGGGQKGPWKQNDSV
                     FNYIDDPTVALDAHGGGYIAWVDNDAKEVLVQRFEPSGWRRRAVNVSRTPTVFSWLPR
                     LVISPRDPKRVFVLWQEMVFSGGSHGGEIYFARSVDGGRTFETPINVSQSPNGDGNAR
                     IEPDHLLNGSLDLIADTGGTLYAAWSDYEGTLWFSRSADDGSHFTPPLPIVINRTRPA
                     CAPALATGGGVIYLAWTIGEDPRADIHLATSSDGGETFTPAIVVSRTAGYSGAPKIAV
                     DVRGTLHLVHSESRDGPFGRSHIRYMRSVDHGRTFEAPREISAPPPHDATSAAFPSLA
                     LDGERVIVTWELYPALEHPRGLALAFSADGGVTFARPTMIPGSRDSGGGDNGSLQGRL
                     MRKLAVGDNGLAVVNSAMLGDVRSRVWLIRGRWR"
     gene            complement(2470..3408)
                     /locus_tag="VFV99_31075"
     CDS             complement(2470..3408)
                     /locus_tag="VFV99_31075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063878.1"
                     /translation="MHRLAIIALLSGCSTGIDYDHFASALVDARCAYFVRCGVAASAT
                     ECRAFYERVAVGNPSTNAAIDDGKVAYHSDVAQDCFDAYAALSCDQTLQTGDELTVCN
                     GVLTGTVTSGGTCAFSAECESKNCVVPSCTMACCTGMCAEPQVLPGRGEACTALCADG
                     LFCDVDSTCKEPLPEGAACSAQPCAYGLYCAGLTPTMAGRCTKLPHIGQPCSTACAEV
                     GATCYGGSCVAFALKDDACTADAQCSMFYECNSMQCTLLPTLAMPCRTTCYEAAYCDG
                     TTCIAQKGNGMTCLRNDECESHLCQMGVCADPPVCL"
     gene            3443..5656
                     /locus_tag="VFV99_31080"
     CDS             3443..5656
                     /locus_tag="VFV99_31080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX5063879.1"
                     /translation="MAGEPEVTLDGTLERITYRGEEYYTVARFTTDKGEPITIVGKLV
                     EINEGMPITVVGQWVVDKKYGRQFKVSFAHPRTPKTVIGIEKFLGSGLIHGIGPSIAK
                     RIVEKFGKETLEVIEKTPTRLTEVVGIGTQRAKQLADAVAAQRHVQEVMVFLQGHGVT
                     MAQAARIVRKYGKDAINVVRANPYRLATEVWGIGFRTADSIAEKLGIARDADERLEAG
                     LLHALETSVEDGHLHLPDDELLAAAAELLQIDVAKLPPRLGALEASKKIVREVLGNRG
                     ACSELPELYTAEVESANLLAELAASPAKSFPLDVGAAIHAFESVTGLELANQQRQAVE
                     AALRDRCVVITGGPGVGKTTIVKAIVHLARIGHRRVALAAPTGRAAKRLSEATAAEAM
                     TIHRLLEFQPHEGGFQRTRENPLDADVLVVDEASMVDALLFRSVLGALRPGAQLVLVG
                     DIDQLPSVGAGAVLADVIDSGAATVIRLTEIFRQAAESKIVVSAHKINRGEIPDLDVA
                     GDKNDFYFITREEPDAARATIVELVAERIPARFGFDAITDVQVLTPMHRGELGTAALN
                     KALQERLNPSSGGAELVRGDRAFRRGDKVMQLKNDYDRNVFNGDIGVIEGIDLAGQSE
                     SGPLHSGGSGTLRVDFDGRPASYERAELDQLVHAYAVSVHKSQGSEYPAVVIPLATQH
                     FMMLQRSLLYTAVTRGKKLVVLVGSKRAVQLAVRNADAKKRYTWLAERVRAAMAS"
     gene            5738..6019
                     /locus_tag="VFV99_31085"
     CDS             5738..6019
                     /locus_tag="VFV99_31085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063880.1"
                     /translation="MGRLDDIIDRNKHPRKHSKMRFPLGIMLSAFVLLILVLMIFTDL
                     ADSPDSKTQQTQSPAPSGEKRVDGVLLYREKTHRDAGAATGSATTQPRP"
BASE COUNT         1035 a         1982 c         2078 g          972 t
ORIGIN      
        1 caggagcgtc cccaccgctc gtcaagaccg cgatgcgttt catgggcccg atgctacagt
       61 cggcgaatgg tccgagtcga acgatcaggc ccggtgacca ctgtcatcct cgatcgtgcg
      121 gcggccaaga atgccgtcga ccgcgacacc gcgcaggcac tcgccgatgc gtttcgcaac
      181 ttcgacgccg attcgtcggc ccgcgtcggt gtcctctggg gtgaccacgg tacgttttgc
      241 gcgggcgccg atctcaaggc gctcgccgcc ggtacaccga atcgcgtcgc gcccgacggc
      301 gacggcccga tgggtccgac ccgcatgctg ctcggcaagc cggtgatcgc ggccatttcg
      361 ggccacgcgg tcgcgggtgg gctcgagctc gcgctgtggt gcgacctgcg tgttgcggaa
      421 gacaacgcca cgctcggcgt gttctgccgg cgcttcggag ttcctctgat cgacggcgga
      481 acaatccgcc tgccgcgctt gatcggtttg tcgcgcgcgc tagacctcat cctcactggt
      541 cgcgccgtga aagcagatga agcgttgatg atggggctcg tcaatcgcgt cgtgccgccg
      601 ggccaggccc gtgctgccgc cgagcagctc gcacacgagc tcgcggagct gccgcaagcg
      661 gcgatgcgcg gcgatcgcat gagtgcctat ctgcaacacg atctcgatct cgaggccgcg
      721 ctgcaaaagg agctcgagct cggctcgcgc gcgctcggcg aagcggtcca aggtgcaacc
      781 cgtttcaccg gcggcgctgg ccgccacgga ggtcgcgaat gatccgcggg ctgttcgccg
      841 catcgatcat catcttgctc acgagctgta tcgtgagaca cctcgtcagc gggccacggc
      901 tgactggcac gtgccatggc gcgtgcgatc actacatcga gtgcaaaccc ggccacgagg
      961 atctcgatcg ccagcgctgc gagaccgagt gcccgggcgt gttcagcgat cgcgattcgc
     1021 tgatggcgta tgaaagcctc tcctgcaaga acgcggtcga gtacgtcgac ggcacgcagc
     1081 cgaggtcggc gaacacggcc cgttaacgcc agcgtccgcg gatcagccat acgcggctgc
     1141 gaacgtcgcc cagcatcgcg ctgttgacga cggcgaggcc gttatcgccg accgcgagct
     1201 tgcgcatgag ccggccttgc aggctcccgt tgtctccgcc acccgagtcg cgactgcccg
     1261 ggatcatcgt cggccgcgcg aacgttacgc cgccgtcggc ggaaaacgcg agcgccaggc
     1321 cgcgcggatg ctcgagtgcc ggatagagct cccaggtcac gatcacccgc tcgccatcga
     1381 gcgcgagcga tggaaaggcg gcgctcgtcg cgtcgtgcgg cggtggcgcg gagatctcgc
     1441 gcggcgcttc gaacgtgcgt ccatgatcga ccgagcgcat gtagcggatg tgcgaacgac
     1501 cgaacggtcc atcgcgcgac tccgagtgta cgaggtgtag cgtgccgcgt acgtcgacgg
     1561 cgatcttcgg cgcgcccgaa taaccggccg ttcggctgac gacgatggcc ggcgtgaacg
     1621 tttcaccgcc atcgctcgag gtcgcgaggt gaatgtcggc gcgcgggtct tcgccgatgg
     1681 tccacgcgag gtagatgaca ccaccaccgg tcgcgagcgc cggtgcgcac gccggtcggg
     1741 tgcggttgat gacgattggc aggggcggcg tgaagtgaga gccgtcgtcc gccgagcggg
     1801 aaaaccacag cgtgccctcg tagtcgctcc acgctgcgta gagcgtgccg ccggtgtcgg
     1861 cgatgagatc gaggctgccg ttgagcaggt gatccggctc gatccgcgca ttgccatcac
     1921 cattgggcga ttgggacacg ttgataggcg tttcgaacgt gcggccaccg tcgaccgagc
     1981 gcgcaaaata gatctcgccg ccgtgggagc cgcccgagaa caccatctcc tgccacagca
     2041 cgaatacgcg cttcggatcg cgcggtgaga tcacgaggcg cggcagccac gagaacacgg
     2101 tcggcgtgcg cgaaacattg accgcgcgcc gccgccagcc gctcggttcg aaacgctgga
     2161 cgagcacttc cttcgcgtcg ttgtcgaccc aggcgatgta gccaccgccg tgtgcgtcga
     2221 gggcgaccgt cgggtcatcg atgtagttga agaccgaatc gttctgcttc cacggcccct
     2281 tctgcccgcc gccgtgtgcg atctcgatgg gtggcgacca ctcgatcggc tcccggatga
     2341 ccggcgccga gcatgccgcc agcaccagca accaggtcct catggactag tttaccgtcg
     2401 agaaatggac gcaaccattc ggtgcgtttc atacgcagtg cccacctgac acaggtggct
     2461 ttcgcaaagc tagaggcaga ccggcggatc cgcgcagaca cccatctgac acaagtggga
     2521 ttcgcattcg tcgttgcgca ggcaggtcat cccgttgcct ttctgagcga tgcatgtcgt
     2581 gccgtcgcaa tacgcggcct cgtagcacgt ggtccggcac ggcatcgcga gcgtcggcag
     2641 cagcgtgcat tgcatgctgt tgcactcgta gaacatcgag cattgcgcgt ccgcagtgca
     2701 tgcatcgtcc ttcagtgcga acgcgacgca gctgccgccg taacaggtcg cgccaacttc
     2761 tgcgcacgcg gtcgagcacg gttgaccgat gtgcggcagc ttcgtgcacc gtcccgccat
     2821 cgtcggtgtg agcccggcgc agtagaggcc gtacgcgcag ggctgggcgg agcacgcggc
     2881 gccctcgggc agcggctcct tgcacgtgct gtcgacgtcg cagaacaggc cgtccgcaca
     2941 cagcgcggtg cacgcttcgc cacggccggg gagcacttgc ggttcggcgc acatgccggt
     3001 gcagcacgcc atcgtgcacg acggcaccac gcaattcttc gattcacact cggcgctgaa
     3061 cgcgcaggtg ccgccgctcg tgacggttcc ggtgagcacg ccgttgcaga ccgtgagctc
     3121 gtcgccggtc tggagcgtct ggtcgcacga cagcgcggcg tacgcgtcga aacagtcttg
     3181 cgcgacgtcc gagtggtacg cgaccttgcc gtcgtcgatc gcggcgttcg tgctcgggtt
     3241 cccgacggcc acgcgctcgt agaacgcccg gcactcggtg gcggaggcgg ccacgccgca
     3301 gcgcacgaag taggcgcacc gcgcgtcgac gagggcgctc gcgaagtggt cgtaatcgat
     3361 gccggtgctg cagcccgaaa gcagcgcgat gatagcgagc cggtgcatcg tcagcatcgt
     3421 accgcagcgc tagtatgcga gggtggcagg cgagcccgaa gtcacgctcg acgggacgct
     3481 cgaacggatc acgtatcgag gcgaggagta ctacaccgtc gcgcgcttca cgaccgacaa
     3541 gggcgagccg atcacgatcg taggcaagct cgtcgagatc aacgagggca tgccgatcac
     3601 cgtggtcggg cagtgggtcg tcgacaagaa gtacgggcgg cagttcaagg tgtcgttcgc
     3661 gcatccgcgc acgccgaaga cggtgatcgg catcgagaag ttcttgggct cgggattgat
     3721 tcacggcatc gggccgagca ttgccaaacg catcgtggag aagttcggca aggaaacgct
     3781 cgaggtcatc gagaagacgc ccacccgatt gaccgaggtc gtcggcatcg gcacgcagcg
     3841 ggcgaagcag ctcgccgacg cggtggcggc acagcgccac gtgcaggaag tgatggtgtt
     3901 cctgcagggc cacggcgtga cgatggcgca ggccgcgcgc atcgtgcgca agtacggcaa
     3961 ggacgcgatc aacgtcgtgc gcgcgaatcc gtaccggctc gccaccgaag tgtggggcat
     4021 cgggtttcgc accgcggatt cgatcgccga gaagctcggc atcgcgcgcg acgccgacga
     4081 gcgcctcgag gccgggctgc tgcacgcgct cgagacgagc gtcgaggacg gccatctaca
     4141 tctacccgac gacgagttgc tcgccgccgc tgcggagctg ttgcagatcg acgtcgcgaa
     4201 gttgccgccg cgactcggcg cgctcgaggc gagcaagaag atcgtgcgcg aggtgctcgg
     4261 caaccgcggc gcgtgtagcg agctgcccga gctgtatacc gcggaggtcg agtcggcgaa
     4321 cctgctcgcc gagctggcgg cgtcgccggc gaagagcttt ccgctcgacg tcggggctgc
     4381 gatccacgca ttcgagtcgg tgaccggtct cgagctggcg aaccagcaac gccaggccgt
     4441 cgaggcagcg ctgcgcgatc gctgcgtcgt gatcacgggt ggtcccggcg tcggcaagac
     4501 aacgatcgtg aaagcgatcg tccatctcgc gcggatcggc catcggcgcg tcgcgctcgc
     4561 ggcaccgacg ggacgcgcgg cgaagcggct ctccgaggcg acagcggcgg aggcaatgac
     4621 gatccaccgg ctgctcgagt tccagccgca cgaaggcggg tttcagcgca cgcgcgagaa
     4681 tccgctcgat gccgatgtgc tcgtcgtcga cgaggcgtcg atggtcgacg cgctgctgtt
     4741 tcgctcggtg ctcggcgcgc tgcggccagg tgcgcagctc gtgcttgtcg gcgacatcga
     4801 tcagttgccc tcggtcggcg ccggcgcggt gctcgcggac gtcatcgatt cgggtgcggc
     4861 gacggtgatc cggctgaccg agatcttccg ccaggcggcg gagagcaaga tcgtggtgtc
     4921 agcgcacaag atcaatcgcg gcgagatacc cgacctcgac gtcgcgggtg acaagaacga
     4981 cttctacttc atcacgcgcg aggagcccga cgcggcgcgc gcgacgatcg tcgagctggt
     5041 cgccgagcgg atcccggcgc gctttggttt cgacgcgatc accgacgtcc aggtgctgac
     5101 gcccatgcat cgcggcgagc ttggcaccgc ggcgctcaac aaggcgctgc aagagcgact
     5161 caacccgagc agcggtggtg ccgagctggt gcgcggcgac cgcgcgtttc gccgcggcga
     5221 caaggtgatg cagctcaaga acgactacga ccgcaacgta ttcaacggcg acatcggcgt
     5281 gatcgagggt atcgacctcg cgggccagag tgaatctggc ccgctccatt ccgggggctc
     5341 gggcacgctg cgcgtcgact tcgacggccg gccggcgagc tacgagcgtg ccgagctcga
     5401 ccagctcgtc cacgcctatg cggtcagcgt ccacaagagc cagggctccg agtatcccgc
     5461 ggtggtgatt ccgctcgcga cccaacactt catgatgctg cagcggagcc tcttgtacac
     5521 ggcggtcacg cgcggcaaga agctcgtcgt cctcgtcggc agcaagcgcg ccgtgcagct
     5581 cgcggtgcgc aacgcggatg cgaagaagcg gtacacctgg ctcgccgagc gcgtccgagc
     5641 ggcgatggcc agttagtgtc cgagctgcga tggccgacta gcgtcctgcg aggacgagcc
     5701 agcggcggcc ggggcgccgc cgtgccatag tccgcgcgtg ggtcggctcg acgacatcat
     5761 cgatcgcaac aagcacccgc ggaagcacag caagatgcgc tttccgctcg ggatcatgct
     5821 gtcggcgttc gtgctgctga tcctcgtgct gatgatcttc acggatctcg ctgactctcc
     5881 cgactcgaag acgcagcaga cacagtcacc ggcgccgagt ggtgagaagc gcgtcgatgg
     5941 cgtcctcttg tatcgcgaga agacgcatcg cgatgcaggc gcggcgacgg gcagcgcgac
     6001 gactcagccg cgcccctaac cgcggtagac gtcgtcctgc tcgatcttcg acagcgtcca
     6061 gtcgaag
//
LOCUS       DASUFX010000770         7297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_542310,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000770 DASUFX010000000
VERSION     DASUFX010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542310"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFV99_31090"
     CDS             complement(<1..543)
                     /locus_tag="VFV99_31090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063881.1"
                     /translation="MRIAASLFFLAIASPAFADDGKGLSLDFGYARNRVAVTDQTTLG
                     AESARFGIRVSLGRHFHFGGEAEEGRIAGTTPLPAGAIARTSTSSEPASPLDGNTLGL
                     KAYVGLHADAGRMMFGADLATGVRDTWVDSDAGRGIAGRANELLLEARARADLSLTDA
                     ISFGVVASTDVIERRDVSLAG"
     gene            782..1441
                     /locus_tag="VFV99_31095"
     CDS             782..1441
                     /locus_tag="VFV99_31095"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX5063882.1"
                     /translation="MRRNLLLACGVLASLLYVATDVAAAICYPAYHSFTDKVISELMA
                     TGAPTERLVDPPLLVYDVLMMAFAVGIWMSLPTRRGHVAAGLLFAYGALGLLGPTVFE
                     MNVRGSGAPPMADYLHIAITGVLVLIIFAIVATASSLRGRAFRIYSYATIVAMIAFGI
                     VTSLLSNGLGTAEPTPWLGAAERLNVGAFLMWVAVLAISLLRVDVHFGSAWMNAKARA
                     R"
     gene            1454..1873
                     /locus_tag="VFV99_31100"
     CDS             1454..1873
                     /locus_tag="VFV99_31100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063883.1"
                     /translation="MHQVVIIALPLAVLAWARSVRVTIAFAVATAVMWVSAAVLADWF
                     FNQPEIFATLVFAYFLLPFVIAPICGVLAAVLIGPPRHLFASGCAAVAGCIGAIAVIG
                     AAGPIVDLRECFHMMAAPTLLAICAATLVAVLAQRRV"
     gene            1988..2716
                     /locus_tag="VFV99_31105"
     CDS             1988..2716
                     /locus_tag="VFV99_31105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063884.1"
                     /translation="MSSKLAWVSALLAANVMTASAQPGAMPPLSSEDGESPATDDSAL
                     APPSSVEVAPIRSVPIRKSPLATTERWGGGGRVTGLSGIGALPGVNYGGEVSGMVRRD
                     EMFAELGFGWWKPEKTYLVAEQPDRVELGLKVWTLRAGWASMTTPLRAWGLVEVGELA
                     SAHGMPGVVTRMMTGDTPQERQWHAVGGGFGVAWPMSDNARLFGMIEIAVPVERERLM
                     LDTGAYEPDPMAARSSAGIELGWR"
     gene            complement(2724..3710)
                     /locus_tag="VFV99_31110"
     CDS             complement(2724..3710)
                     /locus_tag="VFV99_31110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LYR motif-containing protein"
                     /protein_id="HEX5063885.1"
                     /translation="MPRGSWGRTWLVVAVIVAALVVRYELFLRAHGYRPSVKDDEYAW
                     SWERMRAADGSQRTVAWLGASRIMLAFAPEAFTQTLPDWKYVQLAIDGTTPIGSLLDL
                     AADPDFRGVVIFDISELGFQREHWSEQDKHIATYRRRWREVGAMLERWLATNVQSRLA
                     LTATRTMYLTGIALRTGSWPAPPYLTTYADRTRFADYAIADVPRLRKSRVALVHEWVL
                     TMRDPKAWLDDALALEPAIAAIEARGGRVVYVRMPTCDERWDAEEKMTPKAQFWDVLA
                     TRTRAVTVHFKDYPQLAKFPCPDTSHIDSKDGPEFTRAFLEILRARGVFDRR"
     gene            complement(3718..5088)
                     /locus_tag="VFV99_31115"
     CDS             complement(3718..5088)
                     /locus_tag="VFV99_31115"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEX5063886.1"
                     /translation="MLAVHHAPLPWTVRKIALLVFSYAFYAAWDPPFVLLLWGSTVVD
                     WFVAKAMARSERKGRRKALLLISLGFNLSLLGYFKYAGFLARTFASIAGSVGFHWTPT
                     DLGIVLPIGISFYTFMTISYVLDVYLRRAAPARSFLDYALYVTFFPHLVSGPIVRATE
                     LIPQFESPRRANADQFGWGLALLALGLFEKVVIADGILAPVVDKVYAPVLEHVTSTDA
                     WAGTLAFAGQIFCDFAGYSTAAIGVSLCLGFALPDNFRFPYGAIGFSDFWRRWHITLS
                     AWLRDYLYIPLGGNRHGPTRTHVNLMLTMLIGGLWHGASWTFVVWGGLHGLYLVGERV
                     AKRLVQPTPVWETPFARAFLALLTFTLVCFAWVFFRATTFTQAFTIVRAMFGDGQGVA
                     QVTSPLPLIPVFALVAAHMALHRTTLEAVVERTPWPTRAIVLSIFVVCLFLFPGIDRA
                     FIYFQF"
     gene            complement(5150..5641)
                     /gene="ybaK"
                     /locus_tag="VFV99_31120"
     CDS             complement(5150..5641)
                     /gene="ybaK"
                     /locus_tag="VFV99_31120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX5063887.1"
                     /translation="MAKHPVTMAIRALRAAKVAFEPHVYAWQPHGGTRASSEALGVDE
                     HAVIKTLIMEDERKQPLCVLMHGDREVSTKNLARVIGVKSITPCAPEVADRHSGYQVG
                     GTSPFGLRRAMPVYMQQSILDLPRIYINGGARGFLVAIDPKDAQRVLAPSLVDVAVGD
                     DFG"
     gene            complement(5660..6469)
                     /locus_tag="VFV99_31125"
     CDS             complement(5660..6469)
                     /locus_tag="VFV99_31125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063888.1"
                     /translation="MAFVLAAVVIVAGLVAATELMFRLGQARARRHADEKKAEVHVAM
                     REQVGTVQVATLGLLALMLGFTMSMAESRFNQRRVVNVAESNAVGTTFLRANLLTEPE
                     RGESRDVLRKYVAQRVAYFQAKNGDEAAAASSRAQALSSHLFALATKVGQLHPDWDVI
                     ATYIESLNDMIDLEATRDFATRSHLPALIHIVLILIALAAIGVTGYATGLSGARSAFG
                     LYVIPLLVALTCVMILDLDHNRWGLIRNSGIPMQSAQQTIERELGVTNAAR"
     gene            complement(6540..7046)
                     /locus_tag="VFV99_31130"
                     /pseudo
     CDS             complement(6540..7046)
                     /locus_tag="VFV99_31130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         1278 a         2392 c         2462 g         1165 t
ORIGIN      
        1 gcccgcgagc gacacgtcgc gccgctcgat cacgtcggtg ctcgcgacca cgccgaacga
       61 gatcgcatcc gtcaatgata ggtctgcgcg agcgcgtgcc tcgagcagca gctcgttcgc
      121 gcggccagcg atgccgcgcc cggcgtcgct gtcgacccac gtgtcgcgca cgcccgtcgc
      181 gaggtccgcg ccgaacatca tccggccggc gtcggcgtgg aggccgacat acgctttgag
      241 gcccagcgtg ttgccgtcga gcggactcgc gggttccgac gacgtgctcg tgcgcgcgat
      301 cgcaccggcg ggtagcggcg tcgtcccggc aatccggcct tcctccgctt cgccgccgaa
      361 atgaaagtgg cggccgagcg agacgcggat gccgaaccga gcgctctcgg caccgagcgt
      421 cgtctgatcg gtaacggcca cgcgattgcg ggcgtagcca aagtcgagcg acagaccttt
      481 gccgtcgtcc gcaaatgccg gcgatgcgat ggcgagaaag aagagcgacg ccgcgatccg
      541 catgaccaga agcctagccg gcgtttgcca atccgccgct ggcagccgcc ggcaataccg
      601 ctgtgacagc gcctgacctg cgacgcggat gggtctcggc tgcgacagcc tggcgccgta
      661 ccgagaacgc gcggattcag cacggcgaga tcacagatac gcgtggcttg cgtacgcgca
      721 gtgcgtggaa gcgcttactc gtgcgcacgc ctgatgcatt cacggcggtg aggagtcgac
      781 catgcgacgc aacctgctac tcgcgtgcgg tgtcctcgca tcgttgctct acgtcgccac
      841 cgacgtagcg gcggcgatct gctaccccgc gtatcacagc ttcacggaca aggtgatcag
      901 cgagctgatg gccaccggcg cgccgaccga acgtctcgtc gatccgccac tgctcgtcta
      961 cgatgtcctg atgatggcat tcgccgtcgg catctggatg tcgctgccta cccgccgcgg
     1021 ccacgtcgcc gccggcctgt tgttcgccta cggcgcgctt ggcttgctcg gcccgaccgt
     1081 gttcgagatg aacgtgcgcg gctccggcgc cccgccgatg gccgattacc ttcacatcgc
     1141 gatcaccggc gtcctcgtgc tgatcatctt cgcgatcgtc gcgacggctt catcgctgcg
     1201 cggccgtgcc ttccggatct actcgtacgc gaccatcgtg gcgatgatcg cgttcggcat
     1261 cgtgaccagc ttgctctcca acggcctcgg cactgccgaa ccgacgccat ggctgggcgc
     1321 ggcagagcgc ctcaacgttg gcgcgttctt gatgtgggtc gcggtccttg ccatctcgct
     1381 cctgcgcgtc gacgttcact tcggatctgc gtggatgaat gccaaagcgc gagcgcgcta
     1441 gactaccggt cctatgcatc aggtcgtgat catcgcgctt ccgcttgcag tgctggcctg
     1501 ggcgcggtcc gtccgagtca ccatcgcgtt cgcggtggcg acggcggtaa tgtgggtctc
     1561 tgcggcggtg ctcgcggact ggttcttcaa ccagcccgag atcttcgcca cgctggtgtt
     1621 cgcgtacttc ctcctgccat tcgtgatcgc acccatctgc ggcgtgcttg cggccgtgtt
     1681 gatcgggccg ccgaggcacc tatttgcgtc gggttgcgcg gccgtggcgg ggtgtattgg
     1741 cgccatcgcg gtgatcggtg ccgccgggcc gatcgtcgac ttgcgcgagt gttttcacat
     1801 gatggccgct ccgactctcc tcgcgatctg cgccgccact ctggtcgcgg tgctcgcgca
     1861 gcggcgggtc tagctcaccg cggcaaaggg cggcagccgg cactccggat cgtccgtgac
     1921 gggatcgatc gcacgtgcgc actaacttgc ggctagtaag atgaatgcca cggagggtcc
     1981 gatgtccatg tcttcgaagc tcgcgtgggt gagcgcgctg cttgcggcaa acgtgatgac
     2041 ggcgagtgcg cagcccggtg caatgccgcc gctgagcagt gaggatggcg agtctccggc
     2101 gaccgacgac agtgcactcg caccgccgag ctcggtcgag gtcgcgccga ttcgcagcgt
     2161 gccgattcgc aagagcccgc tcgcgacaac ggaacggtgg ggcggcggcg gacgcgtgac
     2221 cggactgtct ggcatcggcg cattgcccgg cgtgaactac ggcggcgagg tctcgggcat
     2281 ggtgcgccgc gacgagatgt tcgccgagct cggttttggc tggtggaagc cggagaagac
     2341 gtacctcgta gccgagcagc ccgatcgcgt cgagctcgga ctcaaggtgt ggacgttgcg
     2401 tgcgggctgg gcatcgatga cgacgccgct gcgcgcgtgg ggcttggtcg aagtcggcga
     2461 gctcgcgagc gcgcacggca tgcccggcgt ggtgacgcgc atgatgaccg gcgacacgcc
     2521 gcaggagcgg cagtggcacg cggtcggcgg cggcttcggt gtcgcgtggc cgatgagcga
     2581 caacgcgcgg ctgttcggca tgatcgagat cgcagtgccg gtcgagcgcg agcggctgat
     2641 gctcgatacg ggcgcgtacg aacctgatcc gatggcggcg cgttcgtcgg cgggcatcga
     2701 gctcggctgg cgctaacggc gcgctagcgg cgatcgaaca cgccacgtgc gcgcaggatc
     2761 tcgaggaacg cgcgcgtgaa ctcgggcccg tccttcgagt cgatatgcga cgtgtcgggg
     2821 cacgggaact tcgcgagctg tgggtaatct ttgaagtgaa cggtgaccgc gcgcgtgcgg
     2881 gtcgcgagca cgtcccagaa ctgcgccttc ggtgtcatct tctcttcggc gtcccagcgc
     2941 tcgtcgcacg tcggcatgcg cacgtacacg acgcgaccgc cgcgcgcttc gatcgcagcg
     3001 attgccggtt cgagcgcgag cgcgtcgtcc agccatgctt tcggatcacg catcgtcaga
     3061 acccattcat ggaccagagc cacgcgtgat ttgcggaggc gaggaacgtc tgcgatcgca
     3121 tagtcggcaa accgcgtgcg gtctgcgtac gtcgtgagat atggcggcgc cggccacgaa
     3181 ccggtgcgga gcgctatgcc ggtcagatac atcgttcgcg tcgcggtgag tgcgagccga
     3241 gactgcacgt tagtcgcgag ccagcgttcc agcatcgcac cgacttcacg ccagcgccgg
     3301 cgatacgtgg cgatgtgctt gtcctgctcg gaccagtgct cgcgttgaaa gccaagctcg
     3361 ctgatatcga agatgacgac cccgcggaag tccgggtccg cggcgagatc gagcaacgaa
     3421 ccgattggtg tcgtgccatc gatcgcgagc tggacgtact tccagtccgg aagtgtctgc
     3481 gtaaaggcct ctggtgcaaa cgcgagcatg atgcgcgagg cgccgagcca cgcgaccgta
     3541 cgctgcgaac cgtcggcggc ccgcatccgt tcccacgacc acgcgtactc gtcgtccttc
     3601 actgagggcc gatagccgtg cgcgcggaga aacagctcgt agcgcacgac gagcgcggcg
     3661 acgatcacgg cgacgaccag ccaggttctc ccccagctgc cgcgtggcac cacgcgctca
     3721 aaactggaag tagatgaagg cacgatcgat cccgggaaag aggaacagac agacaacgaa
     3781 gatggagagc acgatggcgc gcgtcggcca cggtgtgcgt tcgacgaccg cttcgagcgt
     3841 cgtcctgtgc aacgccatgt gcgcagcgac gagcgcgaac acggggatta gcggcaacgg
     3901 actcgtaacc tgcgcgacgc cctggccgtc gccgaacatc gcgcgcacga tggtgaacgc
     3961 ttgcgtgaac gtggtcgcgc ggaagaacac ccacgcgaag cacacgagcg tgaacgtgag
     4021 cagcgcgagg aatgcacgcg cgaacggcgt ctcccacacc ggcgttggct gtacgagtcg
     4081 cttggcgact cgctcgccga cgagatacag cccgtgcaac ccgccccaca cgacaaacgt
     4141 ccacgaggcg ccgtgccaca ggccgccgat cagcatcgtc aacatgaggt tgacgtgcgt
     4201 gcgcgtcggg ccgtgacgat tgccgccgag cgggatgtag aggtagtcgc gaagccaggc
     4261 ggacagcgtg atgtgccagc gtcgccagaa atcggagaag ccgatcgcgc cgtacgggaa
     4321 ccgaaagttg tcgggcagcg cgaagccgag gcagagcgag acgccgatcg cggcggtcga
     4381 gtagccggcg aagtcgcaga agatctggcc ggcgaacgcg agagtgcccg cccacgcgtc
     4441 ggtcgacgtc acatgctcga gcacgggcgc gtagaccttg tcgacgaccg gcgcgaggat
     4501 gccgtccgcg atcacgacct tctcgaacag gccgagcgcg agcaacgcga gcccccagcc
     4561 gaactgatcg gcgttcgcgc gacgcggcga ctcgaattgg ggaatcagct cggtcgcccg
     4621 cacgatcggc cccgacacga gatgcgggaa gaacgtgacg tagagcgcgt agtcgaggaa
     4681 cgagcgcgcg ggtgccgcac gacgcagata cacgtcgagg acgtacgaga tcgtcatgaa
     4741 cgtgtagaac gagatcccga tcggcaacac gatgccgaga tcggtcggcg tccagtgaaa
     4801 accgaccgag cccgcgatcg atgcgaacgt acgcgcgagg aaaccggcgt acttgaagta
     4861 gccgagcagc gagaggttga aaccgagact gatgaggagc agcgccttac gcctgccctt
     4921 ccgttcgctg cgggccatcg ctttcgcgac gaaccaatcg accaccgtcg agccccagag
     4981 cagcagcacg aacggcgggt cccaggccgc gtagaacgcg tagctgaaca cgagcagcgc
     5041 gatcttccgg acggtccacg gcagcggcgc gtggtggaca gccagcacta cgagaaagaa
     5101 gcagccgaag aggtacgtgt tgaactgcac gcgctacggg gcgcgattgt tacccgaagt
     5161 cgtccccgac cgcgacgtcg acgagggagg gcgcgagcac gcgctgtgcg tccttcggat
     5221 cgatcgctac gagaaaaccg cgcgcaccgc cattgatgta gatccgcggc aggtcgagga
     5281 tgctttgctg catgtagacc ggcatcgcgc ggcgcaatcc gaacggcgat gtgccgccga
     5341 cctgatagcc cgagtgacgg tccgcgacct cgggcgcgca cggcgtgatc gatttgacac
     5401 cgatcacgcg cgcgagattc ttcgtcgaga cttcgcgatc cccgtgcatc aggacacaga
     5461 gcggctgctt gcgctcgtct tccatgatca gcgtcttgat cacggcatgc tcgtcgacac
     5521 cgagcgcctc gctcgatgcg cgcgtaccgc cgtgcggctg ccacgcgtaa acgtgcggct
     5581 cgaacgcgac cttggcggcg cgcagcgcac gaatcgccat cgtgaccggg tgcttcgcca
     5641 tgcgcgcggc gattgtaggt caacgcgcgg cgttcgtgac gccgagctcg cgctcgatcg
     5701 tttgctgcgc gctctgcatc ggaatcccgc tgttcctgat gagtccccac cgattgtggt
     5761 cgaggtcgag aatcatcacg cacgtgagcg cgaccagaag cgggatcacg tagagcccga
     5821 atgcgctccg cgcccctgac aggcccgtcg cgtagccggt gacgccgatc gcggcgagcg
     5881 cgatcaggat caagacgatg tgaatcagcg cgggcaaatg cgagcgggtc gcgaaatcgc
     5941 gcgtcgcctc gagatcgatc atgtcgttca gcgattcgat gtacgtcgcg atcacatccc
     6001 agtcgggatg gagctgaccc actttcgtcg cgagcgcaaa caggtgcgac gagagcgctt
     6061 gcgcgcgcga ggatgccgct gccgcctcgt caccgttctt cgcctggaaa tacgcgacgc
     6121 gctgggcgac gtacttgcgc aacacgtcac gactctcgcc ccgctccggc tcggtgagga
     6181 gattcgcgcg caaaaacgtc gttccgaccg cgttcgactc ggcgacattg acgacgcgac
     6241 gctgattgaa gcgcgactcc gccatcgaca tcgtgaaacc gagcatcagc gcgagcaggc
     6301 cgagcgtcgc gacctgtacc gtgccgacct gctcacgcat ggccacgtgg acttcggctt
     6361 tcttctcgtc tgcgtgccga cgcgcgcgcg cctggccgag gcgaaacatc agctcggtcg
     6421 ccgccaccag gcctgcaacg atgacgaccg ccgccagtac gaacgccatc cccacagcta
     6481 gcatctgcgg acgtgaagct aaaacgtgcg catgcgctta cggcgcgcgg cgtatgccgt
     6541 cagcgcacca tgccgccgtc gaccaaaagg cactggccgg tcacgaacga tgcatcgtcg
     6601 gacgcgagga ccgcgacggt gcgcgcgagc tcgtcgacgc cgccggcccg gccgagcgga
     6661 atcgtcgcca cgatctcggg agcgaccggt tgcgagtcga cgaacccggg aagcaccgcg
     6721 ttcgcacgca caccgcgcgg tgcaagctct tcggcgagca gctggaccca ggcgccaagc
     6781 gcggcgcgaa atacgctcga cgccgtacgc gtgagctcgg ggcgtgccgc cgcgtagctc
     6841 gagacgcaga cgatcgcgcc acctttggtg agatggggcg tcgtgacccg cgcgagccga
     6901 accgcgctgg cgacgatcag ctcgtagccc gtctgccact gctcgtcggt cagcgagagc
     6961 ggcggcccct tgggcgggtg cccggtattc gcgaccacca cgtccaacct gccccacttc
     7021 gcgagcgcag cgtcgacgag agcgcggagc gtgtctcgtc gcgaacgtca ccctgcatgc
     7081 cgatgccgcc gagctcgctg gcgagcgctt cgacctcggc accgcgggca aggagcgcga
     7141 catgatgacc gcggcgcgcc agctcgcgcg caatcgccgc gccaatgcct ttgcttgcag
     7201 ccgtgacgat cgcgacgcgc gtcatcgcgc cacagtaaca gcgaatcggc ggctatactc
     7261 gcgagctgat gccgtggtgg aagtccatcg cgcgcga
//
LOCUS       DASUFX010000771         8124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_958812,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000771 DASUFX010000000
VERSION     DASUFX010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958812"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /gene="sbcD"
                     /locus_tag="VFV99_31135"
     CDS             complement(<1..439)
                     /gene="sbcD"
                     /locus_tag="VFV99_31135"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEX5063889.1"
                     /translation="MRLLHTSDWHLGHTLKEVTRDYEHAAFLKWLLETCAREEPDVLV
                     ITGDVFDSGTPPASAERMWFEFLAAARRNRPAMDIIAIAGNHDSPARLGAASAVLREL
                     GVHVIGGLPRARDVQGDVATSGSPVGLHLDLDRILIPVAGGRGL"
     gene            562..1092
                     /locus_tag="VFV99_31140"
     CDS             562..1092
                     /locus_tag="VFV99_31140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063890.1"
                     /translation="MQRLACLLIGITAAACGGGTDSGPADLMGTIPPGKSVGARTFAG
                     KDGNMMDVLGWKIEFFEQGPGADCLSSDTKVVARIGIYTSTPDDKMTDQAILKVGDIP
                     IVTASPPPVSGDAAATMSAEGVNGLQGVVSITEFHLADIEHGDRIKGTVTAGGMDGTG
                     NPISVTGSFDAPYCPE"
     gene            complement(1093..1869)
                     /locus_tag="VFV99_31145"
     CDS             complement(1093..1869)
                     /locus_tag="VFV99_31145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063891.1"
                     /translation="MEVFFLMMGLWLVVAVALGLRRPRGLGGAALRARLDAVYGEPHE
                     LVKVSPASFPEADIEFYDRARRELEAKSFSCLGDVEDLTLSRIYPHNRTFMRLFVDAG
                     RMVRASAYHLHPRGVVISLLQLVQLFPRHLRVIELVSEVNGKFLVTSNTHGVDRLEPP
                     PEAKVERMPLATSLLEVVARHEKRITEYLREHPERSPVAFESFEEVLASIARAHVTMA
                     RHRQKVGGLSRDELERLKGRPLSATEEAFLREVQGKGESS"
     gene            1915..2889
                     /locus_tag="VFV99_31150"
     CDS             1915..2889
                     /locus_tag="VFV99_31150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX5063892.1"
                     /translation="MAWPITWDDIVAARRRIASHLTPTPLRHYPALDAEVGNDIRVLV
                     KHENHLPTNAFKARNAMSFMAALPPEQRTRGVVAATRGNHGAGLAWAGLELGVPVVIC
                     VPHGNNPEKNSAMRGFGAEVIEAGRDYDEAAEVAQRLVVDRGLVLAHSTNNRDIIAGA
                     GTLGAELCEQAVTLDAIVVAVGGGSQAVGALVAARHLRPQVAVYGVQAQNAAAIHDSW
                     HARTRLVRDSADTFADGLATRATYDLTFAALQDGLAGFVAVSDPAIADAIRVLLRTTH
                     NLAEGAGAAGLAGVFAMRDQLAGKTVAVVLSGSNIDEPTLRRILAREL"
     gene            2971..3243
                     /locus_tag="VFV99_31155"
     CDS             2971..3243
                     /locus_tag="VFV99_31155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063893.1"
                     /translation="MIKRLVGLAALGGFLYAHKKRGGTMTLDSFKDTARSLVDSAKSK
                     AQDLKLQAEQKMREQRMGAGERSQEIGSDVTGYGSSGYGYDTNIRR"
     gene            complement(3276..3920)
                     /locus_tag="VFV99_31160"
     CDS             complement(3276..3920)
                     /locus_tag="VFV99_31160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX5063894.1"
                     /translation="MRALLVLCLLVRVARADDADAIAKAVPSCDKDRTCFAIQLHIAG
                     DDHGLVATAEWVARQLTAANRHFAPLAIGFQLAGVDTLPASAMHIETRADRDALAENH
                     LGGRVIHVFIVGQLDDVDQEGSIAYGVTWRRPNDARKYLIVSVQALERTLAHELGHFF
                     GLPHSTYAVSIMNKTERAEPPVEQRTFADEEITAMRPVLERLLRNKVIVAVPRR"
     gene            complement(3997..4506)
                     /locus_tag="VFV99_31165"
     CDS             complement(3997..4506)
                     /locus_tag="VFV99_31165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5063895.1"
                     /translation="MSITVREATPADAAALLAHLKALAAEPGINIPLAADEITTTLEQ
                     EKALLADIADSERAIMLIAQAEGQLVGELSLKSISPRRAVRHVATLGMSVKQDWRSKG
                     VGRALMSAVLEWAPTAGIKRIELYVYCRNAPALALYERFGFVVEGRRKAFIREGDEYL
                     DDFVMAKLL"
     gene            complement(4517..5128)
                     /locus_tag="VFV99_31170"
     CDS             complement(4517..5128)
                     /locus_tag="VFV99_31170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX5063896.1"
                     /translation="MSRELRSLLPFEVAIVIAAALVPLPVPAVVPLLIVASISLWIRG
                     RSWTDVIKGPPLYAAIGVLAGATALALALLVSTPLLEAITDYAVQWSMYPVVRGSGGT
                     LVMVMIVVGVSAVAAELVLRGWLIDRVLELRGHPVLAILTGAIAEGLLSEGDLGMRVG
                     AGLFGIGLGWMYVAGGRSVVAPVCARLVFSLGAVGLEALRLVG"
     gene            5221..6120
                     /locus_tag="VFV99_31175"
     CDS             5221..6120
                     /locus_tag="VFV99_31175"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX5063897.1"
                     /translation="MKRTITIAIAFVLFNLAPSLAENAKDAKDAKEAKEAKETKGPTQ
                     DQLSARAKELEKKLKGQGYTILVEPPFVVIGDSPAKEVQRVTSGFLRSKVALLEKEFF
                     TKRPDRVLEVWLFKNRQSFFKGAKKFFDDDPETPYGYYSPDDNALIMNASGLGTLSHE
                     LVHPYMEANFPDVPSWFNEGLASLYEQPSERKGRIVGLPNWRLPNLKKEIKEKTLPKL
                     ATLLGTTRDQFYEANYDAYAYARYLVYYLQEQGKLQEFYKKFLDDKKDLTGATALTAV
                     LGEDLDTFEPKWRKWVMAIPYKR"
     gene            complement(6117..7391)
                     /locus_tag="VFV99_31180"
     CDS             complement(6117..7391)
                     /locus_tag="VFV99_31180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063898.1"
                     /translation="MTAREEFGSYIVYEQLGVGGMARVHVAESRNLRKRVALKRLLAH
                     AADDPELVKSFIHEAKLVGNLKHANVAQTYDFGKVGETYYIAMEFVPGPTVGQLIRQC
                     KATVGIVPFPITLNILCQVCDALAYAHNLKDEKGKSLGLIHRDVSPPNIIISNTGTVK
                     LIDFGVAKLARSTMNTQVGTVKGKFSYMAPEYLNGQLDSRVDLWALGAVAHELLTNRS
                     LFDAPDDVGVLEKVKRAPIPPPSRYNSDVPRDLDAVVLTALERNPQHRWQSAEAMRTA
                     LANVATELKTTVTNAQMMEWIEWAFTQKPGSSDEKTGVSQLIQLLEKPSKPGAKANKA
                     ASSPTLKYDKKNRDRDVDATSPEKPRARKRAISNPHGGVVIIDHSPRRPMLRRSTIGT
                     RVVLWFFLIVLAGAAGFAVYRYGIPHAITRSF"
     gene            complement(7520..8020)
                     /locus_tag="VFV99_31185"
     CDS             complement(7520..8020)
                     /locus_tag="VFV99_31185"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5063899.1"
                     /translation="MMFSRLAAVAVAVLAVGCRDKAREAEPAAEHAHDPGSAATPGSA
                     ATPAIADAGPPANVVQTEMRMLTEILQHTVRGIGVGDVRPIEHELHALHEAKEATEAA
                     VKSGAYKLPKNADKLEQFEAMDEAFHEQLGALVTASTKNDVPAAADALGNLVKACSGC
                     HATFRP"
BASE COUNT         1461 a         2650 c         2711 g         1302 t
ORIGIN      
        1 cgagcccacg cccacccgcg acgggaatga ggatccgatc gagatcgaga tgtagaccga
       61 ccggactgcc gctcgttgcc acgtcgcctt gcacatcacg cgcacgcggc aggccaccga
      121 tcacgtggac gccgagctcg cgaagcactg cagatgcggc accgagccga gccggcgagt
      181 cgtgattgcc ggcgatcgcg atgatgtcca ttgccggccg attccggcgt gcagcggcca
      241 ggaattcgaa ccacatgcgt tcggcactcg ccggcggtgt cccgctgtcg aacacgtcgc
      301 cggtgatgac cagaacgtcg ggctcctcgc gggcacacgt ctccagcagc cacttcaaga
      361 aggcagcgtg ctcgtagtca cgcgtgacct ccttgagggt gtgcccgagg tgccagtcgg
      421 acgtgtggag gagcctcatc ggccgtcgac gatcgcatgg gggtctgacc gtccgttccg
      481 tgtccgccgc ccggccgaag gcgcgcgcag cggggccgat cccatttgca tggccgccac
      541 gccagggata ggttcggcgc catgcaacga ctcgcgtgcc ttctgatcgg gatcaccgca
      601 gccgcgtgcg gaggcggtac ggactccggc cctgcagatc tcatggggac gataccgcct
      661 ggcaaatcgg taggcgcgcg cacgttcgcc ggcaaagacg gcaacatgat ggatgtgctc
      721 ggctggaaga tcgagttctt cgagcaaggc ccgggcgccg actgcctgag cagcgacacg
      781 aaggtcgtcg cgcggatcgg catctacacc agcacgccgg acgacaagat gaccgatcag
      841 gcgatcctga aggtcggcga cattccgatc gtgaccgcgt cgccgccgcc ggtgagcggc
      901 gatgctgccg ccacgatgag cgccgagggc gtcaacggtc ttcagggcgt ggtctcgatc
      961 accgagttcc acctggccga catcgagcac ggcgatcgga tcaagggcac ggtcaccgcc
     1021 ggcggcatgg acggcaccgg caacccgatc tcggtcaccg gctcgttcga cgcgccgtac
     1081 tgccccgagt aatcagctcg attcgccctt cccctgcact tcgcggagga acgcttcctc
     1141 ggtcgccgat agcggccggc ctttcaagcg ctcgagctcg tcacgcgata gcccgccgac
     1201 tttttggcgg tgacgcgcca tcgtgacgtg tgcgcgcgcg atcgacgcga gcacttcctc
     1261 gaagctctcg aacgcgacgg gcgagcgctc ggggtgctcg cgcaaatact cggtgattcg
     1321 cttctcgtgg cgcgcgacga cttcgagcag cgacgtcgcg agcggcatcc gctcgacctt
     1381 cgcttcgggc ggcggctcca accgatcgac gccgtgcgtg ttgctggtga cgagaaactt
     1441 gccgttgacc tcgctgacca gctcgatcac gcgcaaatgg cgcggaaaca gctgcacgag
     1501 ctgcagcagc gagatcacga cgccgcgcgg atgcaggtga tacgcgctcg cgcgcaccat
     1561 gcggcccgca tcgacgaaca gccgcatgaa cgtgcggttg tgcggataga tccgcgacag
     1621 cgtgaggtcc tcgacatcgc cgaggcacga gaagctcttt gcctcgagct cccgccgggc
     1681 gcgatcgtag aactcgatat ccgcctcggg gaacgaggcc ggcgacacct tcacgagctc
     1741 gtgcggctcg ccgtacacgg cgtcgaggcg cgcgcggagt gcggcgccgc ccagcccgcg
     1801 gggccgccgc aatccgaggg cgaccgcgac gacgagccac aggcccatca tgaggaagaa
     1861 gacttccacg ggcacattgt gcccgacccg cgtcggaccg gctacagtgc gcgcgtggcg
     1921 tggccaatca cgtgggacga catcgtcgcg gcgcggcgcc ggatcgcatc gcacctgacg
     1981 ccaacgccgc tgcgccacta cccggcgctc gatgccgagg tcggcaacga catccgcgtg
     2041 ctggtgaagc acgagaatca cttgccgacg aacgcgttca aggcacgcaa cgcgatgtcg
     2101 ttcatggccg cgctcccacc cgagcagcgc acgcgcggtg tcgtcgcggc gacacgcggc
     2161 aaccatggcg ccgggctcgc gtgggccggg ctcgagctcg gcgtaccggt cgtgatctgc
     2221 gtgccgcacg ggaacaaccc cgagaagaac tccgcgatgc gtgggttcgg ggccgaggtg
     2281 atcgaagcgg ggcgcgatta cgacgaagcc gccgaggtcg cccagcgcct cgttgtcgat
     2341 cgcgggctcg tgctcgcgca ctcgacaaac aatcgcgaca tcatcgcggg tgccggcacg
     2401 ctcggcgccg agctgtgcga gcaggcggtc acgctcgacg cgatcgtcgt ggcagtcggg
     2461 ggcggctcgc aagcggttgg cgcactcgtg gcagcgcgcc acctgcggcc gcaagtcgcc
     2521 gtctacggtg tgcaagcgca aaatgccgct gcgatccacg attcgtggca tgcgcgcacg
     2581 cgcctcgtgc gcgacagcgc cgacacattt gccgacgggc tcgcgacacg cgcgacctat
     2641 gacctcacgt tcgctgcgct gcaggacggc ctggcaggct tcgtcgcagt ctccgatccg
     2701 gcaatcgccg atgcgatccg cgtgctgctg cgcacgacgc acaatctcgc cgagggtgcg
     2761 ggcgcagcag gacttgctgg cgtgttcgcg atgcgcgatc agctcgcagg aaaaacggta
     2821 gcggtcgtac tttcgggctc gaacatcgac gagccgacgt tacgccgcat cctcgcgcgc
     2881 gagctctgag cgatcacgct ctcagccgaa cgcgcagacc gtggcggcac gccacttgcc
     2941 tcctagtccg cacaggacaa ggaggactct atgatcaaac gtctagttgg ccttgcggct
     3001 ctcggtggtt ttctctacgc gcacaagaag cgcggcggaa cgatgacgct cgacagcttc
     3061 aaggacaccg cgcgtagcct cgtcgatagc gcgaagtcga aggcgcagga tctcaagctc
     3121 caggccgagc agaagatgcg cgagcagcgc atgggggctg gcgagcgctc gcaggagatt
     3181 ggttctgacg tcaccggcta tggcagctcc ggctacggct acgacacgaa catccggcgt
     3241 taagaccgag gcggtaacag aaccctgtct ctgtttcacc gccgaggcac cgcaacaatc
     3301 accttgttgc ggaggagccg ctcgagaaca ggccgcatcg cggtgatttc ttcatccgcg
     3361 aacgtgcgct gttcgacggg cggctctgct cgttcggtct tgttcatgat gctgaccgca
     3421 taggtcgaat gcgggaggcc gaagaagtga ccgagctcgt gggcgagcgt gcgttcgagc
     3481 gcttgcacgg agacgatgag gtacttgcgg gcgtcgtttg ggcggcgcca cgtgacgccg
     3541 taggcgatcg agccctcctg atcgacatcg tcgagctggc cgacgatgaa cacgtgaatc
     3601 acgcggccac cgaggtggtt ctcggcgagt gcatcgcgat cggcgcgggt ctcgatgtgc
     3661 atcgccgagg ctggcagcgt gtcgacgccg gcgagctgaa agccgatcgc gagcggcgcg
     3721 aagtggcggt tcgcagccgt tagctgtctg gcaacccact ccgcggtcgc gacgagaccg
     3781 tgatcgtcgc ccgcgatgtg gagctggatc gcaaagcacg tgcggtcctt gtcgcagctc
     3841 ggtaccgctt tggcgatcgc gtcggcgtca tcggcgcgag cgacgcgaac gagcaagcac
     3901 aggacgagca gcgcgcgcac gagatccagg atagcgaagc gccgcgcgcg tgccatgacg
     3961 accgctgaca gcgcccccag aactggggtg tcgcactcac aggagcttgg ccatcacgaa
     4021 gtcatcgagg tactcgtcgc cctcgcggat gaacgctttg cgccgtccct cgacgacgaa
     4081 gccaaaccgc tcgtacagcg cgagcgctgg cgcattgcgg caatagacat acagctcgat
     4141 ccgcttgatg ccggcggtcg gtgcccattc gaggacggcg ctcatgagtg cacggccgac
     4201 gcccttgctg cgccagtcct gcttgaccga catgccgagc gtcgcgacgt ggcgaactgc
     4261 gcgacgcggc gaaatcgatt tgagcgacag ctcgccgacg agctggcctt cggcctgcgc
     4321 gatgagcatg atcgcgcgct ccgaatcggc gatgtcagcg agcagcgcct tctcttgctc
     4381 gagcgtcgtc gtgatctcgt cggccgcgag cgggatgttg atgccgggct cggccgcgag
     4441 cgccttgaga tgggcgagca acgcggcggc atcggcggga gtcgcttcgc gaacggtgat
     4501 cgacatcgcg cggactctat cctacgagcc gcagcgcttc gagcccgacg gcgcccagcg
     4561 agaacacgag tcgcgcacag accggcgcga cgacactgcg gccgccggcg acgtacatcc
     4621 agccaaggcc gatgccgaac aggcccgcgc cgacccgcat cccgagatcg ccctcgctga
     4681 gcaggccctc cgcgatcgcg ccggtcagaa tcgcgagcac gggatggcca cgcagctcga
     4741 gcacgcggtc gatgagccag ccgcgcagca cgagctcggc agcgaccgcg ctgacgccga
     4801 cgacgatcat caccatcacg agcgtgccgc cgctgccgcg gacgaccgga tacatcgacc
     4861 actgcacggc gtagtcggtg atcgcttcga gcagcggcgt cgagacgagc agcgcgagtg
     4921 cgagcgcggt ggcccctgcg agcacgccga tcgcggcgta gagcggcggg cccttgatga
     4981 cgtcggtcca gctccggccg cggatccaga gcgagatgct cgcaacgatg agcagcggca
     5041 cgaccgccgg caccggcaat ggcaccaggg cagccgcgat cacgatcgcc acctcgaacg
     5101 ggaggaggct gcggagctcg cgactcatgg tctacagcgt aggggagaca ccggtcaccc
     5161 gggaaacttt gggtcgaaaa gtgcacgcaa cagcgcgaca cttcagctgt ctgcatcacg
     5221 atgaagagga ccatcacgat cgcgatcgcc tttgtgttgt tcaacctcgc accgtctctc
     5281 gcggagaacg cgaaggacgc gaaggacgca aaagaagcga aagaagcgaa ggaaacgaaa
     5341 ggaccgacgc aggaccagct gtcggcgcgc gccaaggagc tcgagaagaa gctgaagggc
     5401 cagggctaca cgatcctggt cgagccgccg ttcgtggtga tcggcgactc gccggcaaaa
     5461 gaagtgcagc gggtgacgag cggctttctg cgcagcaagg tcgcgctgct cgaaaaagaa
     5521 ttcttcacga agcgccccga ccgcgtgctc gaagtctggt tgttcaagaa ccggcagtcg
     5581 ttcttcaagg gcgcaaagaa gttcttcgat gacgatccgg agacgccgta cggttactac
     5641 tcgcccgatg acaacgcgct gatcatgaac gcgtcgggtc tcggcacgct gtcgcacgag
     5701 ctggtgcatc cgtatatgga agcgaacttc cccgacgtac cgtcgtggtt caacgaaggt
     5761 ctcgcgtcgc tctacgagca gccgagcgag cgcaagggtc gcatcgtggg cctgccgaac
     5821 tggcggctgc cgaacctcaa gaaggagatc aaggagaaga cgctgccgaa gctcgcgacg
     5881 ctgctcggca cgacgcgcga tcagttctac gaagcgaact acgatgccta cgcgtacgcg
     5941 cgctatctcg tctactacct acaggagcag ggcaagctcc aagagtttta caagaagttt
     6001 ctcgacgaca agaaggacct gaccggcgcg accgcgctga ccgccgtgct tggcgaggac
     6061 ctcgacacgt tcgagccgaa gtggcgcaag tgggtgatgg caatccccta caagcgctag
     6121 aagctgcgcg tgatcgcgtg cgggatgccg tagcgataca ccgcgaagcc agcggcgccg
     6181 gcgagcacga tcaagaagaa ccacagcacc acgcgggtgc cgatggtgct gcggcggagc
     6241 atcgggcgcc gcggcgaatg gtcgatgatg acgacaccgc catgcggatt cgagatcgcc
     6301 cgcttccgcg cgcgcggctt ctcggggctc gtcgcgtcga cgtcgcggtc gcggttcttc
     6361 ttgtcgtact tgagcgtcgg cgaggacgcg gccttgttcg ctttggcgcc gggcttgctc
     6421 ggtttctcga gcagctggat cagctgcgag acgccggtct tctcgtcgct actgccaggc
     6481 ttctgcgtga acgcccactc gatccactcc atcatctgcg cgttcgtcac cgtggtcttg
     6541 agctcggtcg ctacgttcgc gagcgcggtg cgcatcgcct cggcgctttg ccagcgatgc
     6601 tgcggattgc gttcgagcgc ggtgagcacg accgcgtcga gatcgcgcgg cacgtcggag
     6661 ttgtagcgag acggcggcgg aatcggcgcg cgcttgacct tctcgagcac accgacgtcg
     6721 tcgggagcgt cgaacaggct gcggttcgtc agtagctcgt gcgcgacggc accgagcgcc
     6781 cacaggtcga cgcgcgaatc gagctggccg ttcaaatact cgggcgccat gtagctgaac
     6841 ttgcccttca cggtgccgac ctgcgtgttc atcgtcgagc gcgcgagctt cgcgacgcca
     6901 aaatcgatga gcttcaccgt gccggtgttc gagatgatga tgttcggcgg cgacacgtcg
     6961 cgatgaatca gcccgagcga cttgcccttc tcgtccttga ggttgtgcgc gtaggcgagc
     7021 gcgtcgcaga cctggcacag gatgttgagc gtgatcggaa acggcactat gccgacggtc
     7081 gctttgcact gccggatcag ctggccgacc gtcgggccgg gcacgaactc catcgcgatg
     7141 tagtaggtct cgccgacctt gccgaagtcg taggtctgcg cgacgttcgc gtgcttcagg
     7201 ttgccgacga gcttcgcttc gtggatgaac gatttcacga gctcgggatc atctgccgcg
     7261 tgtgcgagca gccgcttgag cgcgacgcgc ttgcgcaggt tgcgcgactc ggcgacgtgg
     7321 acccgcgcca taccgcccac gccgagctgc tcgtaaacga tgtacgagcc gaactcctcg
     7381 cgcgcagtca cccggccatc gtatccgacc tgcgacagcg ggtgtcacac gaccgcatca
     7441 ccgcgtcacc gagtcccgct cggtgaacgt cgcagcgcgg ctggggctcg cgtatccagt
     7501 tgatgtcgcg tgcagcagat cagggtcgaa acgtggcgtg gcaaccagag caggccttca
     7561 cgagattgcc gagcgcatcg gctgccgcgg gcacgtcgtt cttggtgctc gcggtcacga
     7621 gcgcgccgag ctgctcgtgg aacgcttcgt ccatcgcctc gaactgctcg agcttgtcgg
     7681 cgttcttcgg cagcttgtac gcaccgctct tcactgccgc ctcggtcgct tccttcgcct
     7741 cgtgaagcgc gtggagctcg tgctcgatcg gacgcacatc gccgacgccg ataccgcgca
     7801 ccgtgtgctg aagaatctcg gtcagcatcc gcatctcggt ctgcacgacg ttcgccggcg
     7861 gacctgcgtc ggcgatcgct ggcgtggcgg ccgagccggg cgtcgcggcc gagccaggat
     7921 catgcgcgtg ttcagctgcg ggctcggctt cgcgagcttt gtcgcgacaa ccgacggcga
     7981 gcacggcaac ggccacagca gcgaggcgcg agaacatcat gcgcggcgtt ctatcacgcg
     8041 gatgccgcaa tcacgaacgt cgccaggatc aggccgaacg aatcattcat ggcgactctg
     8101 acgaaccacg tcaatcggcg gccc
//
LOCUS       DASUFX010000772         3620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1097235, whole genome shotgun sequence.
ACCESSION   DASUFX010000772 DASUFX010000000
VERSION     DASUFX010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097235"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFV99_31190"
     CDS             <1..262
                     /locus_tag="VFV99_31190"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX5063900.1"
                     /translation="FSIPKIGTVAGCMVTEGKINRKAHLRIIRDAVQVYEGKIGSLRR
                     FKDDVSEVQHGFECGVMVAGWNEVKQGDVIEAYEVIEEAAQL"
     gene            303..734
                     /locus_tag="VFV99_31195"
     CDS             303..734
                     /locus_tag="VFV99_31195"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5063901.1"
                     /translation="MVGLVVYVGMAKISLVIDQSHSLKEKRMVLRRIRDRVRERLHVN
                     INEVGELDNWQRAELGCAVVSGDRAKALELIDDIVRVAMQAGGAQIFAIAKTVVTFDA
                     ESLPSIGFPAPIDDRTGAGDKAQGGDDWIPDEWRDEVNDER"
     gene            724..1152
                     /gene="rbfA"
                     /locus_tag="VFV99_31200"
     CDS             724..1152
                     /gene="rbfA"
                     /locus_tag="VFV99_31200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5063902.1"
                     /translation="MSGKGGGGSARKARVEHAMRETLTELIAADVKDPRVHEPELLTI
                     SRVELNVDMSVARCYVSIVGEDAVADAALEGLNKAAGFLRGPIGRRLGLQRAPELRFM
                     MDPSLDVSEKLAAIVREDEERARAAGREPGESGSSNKEKT"
     gene            1149..2246
                     /locus_tag="VFV99_31205"
     CDS             1149..2246
                     /locus_tag="VFV99_31205"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5063903.1"
                     /translation="MSDDGQRTDGERRTTEPVETVSVVHMLDRACEILRDAKRILLTS
                     HRRPDGDGTGSMAGLASLLRAQGKEAVIYSVDPIARRYKWLPLVAGTVHTIPFDDVFD
                     CTIVVDCADVSLLGDTLPPPEVCGILITLDHHASGHPFGDIPVWDPTAAAVGVLVHRI
                     AMHEGWPITPEAAVPLYVSLISDTGGFRHANTNAEALHVGSELIRAGVVPSTIASSLE
                     ERPSPGKLRLLGAVLSTLELHCAGRAGVLSVTTDMVEGARASWEDIEGMVNWARNVEG
                     VQVGVLLTTAKGGGVRVSMRSRSEKIDVGKVCMGLGGGGHPGAGGCHMSGDLPSTKEK
                     VVRALEQAFNRTSQPVIKAPSQTKTPDTASG"
     gene            2307..3362
                     /locus_tag="VFV99_31210"
     CDS             2307..3362
                     /locus_tag="VFV99_31210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063904.1"
                     /translation="MRWMPLFVVLVACGDPKDKPDAGGVTPDTPVVVDMGIDADPNNP
                     MTLMDTGLCLDAACTQIAPGIRPYSPQFQLWSDGATKKRWIYLPPGTQIDTSNMDFWQ
                     FPVGTKLWKEFTRDGVRVETRLVMRIAQGSTNPDSDWFYVAFVWNQTQDATTAEPFGV
                     PDANGTQHDVPNRSQCKICHNNTQPSRVLGFSAIQLDHAASSGEVALQTLIDENLLTN
                     NPTSSSTAGGYFPLPGSSIEKAALGYLHANCGHCHNPSSKVYTDNGVLMQLRETVGTL
                     GTLTSTTVYQTAVNQNGTLPQGNITKLVDPGLPDSSLMILRFESTNPALHMPLLGSEI
                     MDPSGQTILRDWVTSLQ"
     gene            3359..>3620
                     /locus_tag="VFV99_31215"
     CDS             3359..>3620
                     /locus_tag="VFV99_31215"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063905.1"
                     /translation="MNGVLVLDKPPGMSSASAVNHVKKRLDAKRAGHGGTLDPIATGV
                     LPICLGEATKLAAYLLADDKEYEAQALLGVETDTLDRTGTVIA"
BASE COUNT          675 a         1161 c         1222 g          562 t
ORIGIN      
        1 gttctcgatc ccgaagatcg gcaccgtcgc cggctgcatg gtcaccgaag gcaagatcaa
       61 ccggaaggcg cacctgcgca tcatccggga cgctgtccag gtgtacgaag gcaagatcgg
      121 ctcgctgcgc cgtttcaagg acgacgtcag cgaggtccag cacggcttcg agtgcggcgt
      181 gatggtcgcc ggctggaacg aagtgaagca gggcgacgtc atcgaagcct acgaagtgat
      241 cgaagaagcg gcgcagctct aagcctctgg ctcagaagtc ctcgcttgcc gacgagccct
      301 acgtggtagg gttggtcgtg tacgtcggaa tggccaagat cagtctggtg atcgatcaga
      361 gccattctct gaaggaaaag cgcatggtcc tgcgccgtat cagggaccgc gtgcgcgaac
      421 gactccacgt caacatcaac gaggtcggcg agctcgataa ctggcagcgt gccgagctcg
      481 gctgcgcggt cgtgagcggc gatcgcgcga aggcgctcga gctgatcgat gacatcgtgc
      541 gcgttgcaat gcaggccggt ggcgcgcaga tcttcgcgat cgcgaagacg gtggtcacgt
      601 tcgacgccga gtcgcttcca tcgatcggat ttccggcacc gatcgacgac cgcaccggtg
      661 cgggggacaa ggcgcagggc ggggacgact ggattcccga cgaatggcgc gacgaggtga
      721 atgatgagcg gtaagggcgg tggcggcagt gcgcggaagg cgcgcgtgga acacgcgatg
      781 cgcgagacgc tcaccgagct gatcgcggcg gacgtcaagg atccacgtgt acacgagccg
      841 gagctgctca cgatctcgcg cgtcgagctc aacgtcgata tgtcggtcgc ccgctgctac
      901 gtgtccatcg tcggcgagga tgcagtggcg gatgccgcgc tcgagggcct gaacaaggcc
      961 gccggttttc tgcgcggccc gatcggtcgg cgcttgggtc tgcaacgcgc gcccgagctt
     1021 cgcttcatga tggatccatc gctcgacgtc agcgagaagc tcgccgcgat cgtgcgtgaa
     1081 gacgaagagc gagcacgcgc agccggccgc gagccgggcg agagcggctc ctcgaacaag
     1141 gagaagacgt gagcgacgac ggtcagcgca ccgatggcga gcgccgaacg accgagccgg
     1201 tcgagaccgt gagcgtcgtg cacatgctcg accgcgcgtg cgagatcctg cgcgacgcca
     1261 agcggatctt gctcacgagc caccgccggc ccgacggcga cggcaccggg tcgatggccg
     1321 ggctcgcgtc gctgctccgc gcgcagggca aagaagcggt gatctactcg gtcgatccga
     1381 tcgcgcgccg ctacaagtgg ctgccgctcg tcgccggtac cgtgcacacg attccgttcg
     1441 acgacgtgtt cgactgcacg atcgttgtcg attgcgccga tgtttcgctg ctcggcgaca
     1501 cgctgccgcc gccggaggtg tgcggcatct tgatcacgct cgatcaccac gcgagcggcc
     1561 atccgttcgg tgacattccg gtgtgggatc cgactgcggc tgccgttggc gtgctcgttc
     1621 atcgcatcgc gatgcacgaa ggttggccga tcacgcccga agcggcagtg ccgctctacg
     1681 tctcgctgat cagcgacacc ggcggcttcc gccacgcgaa cacgaacgcc gaggcgctcc
     1741 acgttggctc cgagctgatt cgcgcgggtg ttgttccctc cacgatcgca tcgagcctcg
     1801 aggagcgacc gagcccgggc aagctgcgcc tgctcggtgc ggtgctgtcg acgctcgagc
     1861 tgcactgcgc cggacgcgcc ggcgtgctca gcgtgacgac cgacatggtc gaaggcgcac
     1921 gtgcctcgtg ggaggacatc gaaggcatgg tcaactgggc gcgcaacgtc gaaggcgtcc
     1981 aggtcggcgt gctgctgacc accgcgaagg gtggcggcgt gcgcgtgtcg atgcgctcgc
     2041 gcagcgaaaa gatcgacgtc ggcaaagtct gcatggggct cggtggcggc ggccatccgg
     2101 gggctggtgg ctgccacatg agcggcgatc taccgagcac gaaggagaaa gtcgtgcgcg
     2161 cgctcgagca agcgttcaat cgcacctcgc aacctgtgat caaagctccg tcgcagacga
     2221 agacacccga cacggcgtcc ggataaccgc gttcaccacg agaacactcc gcccaccgcg
     2281 tcgaacgaac gcgataggat gggcccatgc ggtggatgcc actcttcgtg gtgctggtcg
     2341 cgtgcggcga cccgaaagac aagccggacg caggtggcgt gacgcctgat acgccggtcg
     2401 tcgtcgatat ggggatcgat gcggatccga acaacccgat gacgctgatg gacaccgggt
     2461 tgtgtctcga cgctgcgtgc acgcagatcg cgcccggcat caggccgtac tcgccgcagt
     2521 tccagctgtg gtccgatggc gcgacgaaga agcgctggat ctacctgccg ccgggcacgc
     2581 agatcgatac gagcaacatg gacttctggc agttcccggt cggcacgaag ctgtggaaag
     2641 agttcacgcg cgatggcgtg cgcgtcgaga cccgcctcgt gatgcgcatc gcccagggct
     2701 cgacgaatcc cgacagcgac tggttctacg tcgcgttcgt gtggaaccag acgcaggacg
     2761 cgacgaccgc cgagccgttc ggcgtgcccg acgcgaacgg cacccagcat gacgtgccga
     2821 atcgctcgca gtgcaagatc tgccacaaca acacgcagcc gtcgcgcgtc ctcggcttct
     2881 cggcgatcca gctcgatcat gccgcgtcga gcggcgaggt cgcgctgcag acgctcatcg
     2941 acgagaatct cttgacgaac aacccgacga gcagcagcac ggccggcgga tacttcccgc
     3001 tgccgggcag ctcgatcgaa aaggcggcgc tcggctatct gcacgcgaac tgcggccact
     3061 gccacaaccc gagctccaag gtctacacgg acaacggcgt cctgatgcag ctgcgcgaga
     3121 ccgtgggcac gctcggcacg ctgacgtcga cgacggttta ccagacggcc gtgaaccaga
     3181 acggcacgct gccgcagggc aacatcacga agctcgtcga tccggggctg ccagattcat
     3241 cgctcatgat cctccgcttc gagagcacga acccggcgct tcatatgccg ctgctcggga
     3301 gcgagatcat ggatccgagc ggccagacga tcctgcgcga ctgggtcaca agcctgcagt
     3361 gaacggcgtc cttgtcctcg acaagccgcc gggcatgtcg tcggcgtccg ccgtcaatca
     3421 cgtgaagaag cggctcgacg cgaagcgcgc cggccatggc ggcacgctcg atccgatcgc
     3481 gaccggcgtg ctgccgatct gcctcggtga ggccacgaag ctcgcggcgt acctactcgc
     3541 cgacgacaag gaatacgaag cccaagcgct gctcggcgtc gagaccgaca cgctcgatcg
     3601 caccggcacc gtgatcgcga
//
LOCUS       DASUFX010000773        12665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2070654, whole genome shotgun sequence.
ACCESSION   DASUFX010000773 DASUFX010000000
VERSION     DASUFX010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12665
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070654"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1392)
                     /locus_tag="VFV99_31220"
     CDS             complement(223..1392)
                     /locus_tag="VFV99_31220"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEX5063906.1"
                     /translation="MSEFLAEVISYVELGEREIELLRDLHPKLQPHFPSIAQRFYAAV
                     FEHPGAAAVISGPEQVERLRGSLIDWMSTGLLGPYDDKFYEKRSRVGRRHVQIGLAQQ
                     YMFTAMNVVRTAYLEKIVELYPLDRACTVIRAVNKLLDIELAVMLRHYQLDSEEKLLQ
                     RERRIQSDRLTAMQTLSAGLAHEVRNPLNAARLQLELLERRIRRQVGDDSKLIEPTTL
                     ANQELERLSNLLNDFLSFARPPELHLHEHDLIEIGRHVIELERPLAQQRGVELTFHEV
                     PPIIAQVDSGKIHQVIQNLVRNGLEASTYGGHVGLWITNEGENSVHIRVTDEGSGIPP
                     EVLQRIYEPFFSTKETGTGMGMAIVHSLVSMHGGRIEIDTSTSGTRFDVTVPQRA"
     gene            complement(1411..2826)
                     /locus_tag="VFV99_31225"
     CDS             complement(1411..2826)
                     /locus_tag="VFV99_31225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5063907.1"
                     /translation="MSRSGRILVVDDEVNARTALAELLRDEGFEVETAADAFKALGKY
                     EAFTPHIVVTDLKMPGMDGIELVKKLRAMEDPPSVIVMTAFGAVASAVDAMRAGAAEY
                     LTKPINFDELLVVIDKVMEHLQLKRETRQLRARVRDRVAPSNIIGSSPPMQRVFEITD
                     QVAPSKATVLITGESGTGKELVANAIHARSPRANGPFVKLHCAALAETLLESELFGHE
                     RGAFTGAMARKDGRFSIADGGTLFLDEIGEISPSVQVKLLRFLQEHEFERVGGTQTIK
                     VDVRVIAATNKHLADEVKAGRFREDLYYRLNVVTIDMPPLRDRKSDIPALAKFFLDRY
                     AKDNGKPIETFAPQTLELLMSYDWPGNVRELENAIERAVVLTTGPIVEPRNLPPNVRP
                     TLRPAGMPPIPGSSLAEIERYAILESLKATGGSTSKAADMLGISVRTIQYRLHDYNAA
                     PRSEVSVVASGSTGTNSGSNK"
     gene            2976..4343
                     /gene="hemN"
                     /locus_tag="VFV99_31230"
     CDS             2976..4343
                     /gene="hemN"
                     /locus_tag="VFV99_31230"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX5063908.1"
                     /translation="MTLPAELTPECLAEQSSRGPRYTSYPPATEFAPIDTARVVRELA
                     TVQCEGAPVSLYVHVPFCRSLCWYCGCNVIPSRDTSRGEHYVDVLATELALLAGSLGV
                     GFPVAEIALGGGSPNFLTPRSLRTLKTAIDRSFTVQKTARLSVELDPRDTTATQIDTF
                     ADVGFRVLSVGVQDFSTRVQDAIHRHQTLAQTGWLIERARAAGFTDVNVDMVYGLPRQ
                     HESSFLATLESVIGLGPDRIALFGYAHLPDRLPHQRLVEKAGRVLDRYERATLLLLAI
                     EKLTNAGYIHLGLDHFAKPGSALARAAAAKRMVRTFQGYVEHRADTLLGLGTSAISST
                     PRMYWQNFGDLAPWEDAIGAQRLPVQRGAELDADDRIRRTLIERLMCDGEADLDQLES
                     RFGIAPLDYFARELERLAAEPALANLDRHTIRTTPLGKLLVRNVCMMFDRYHREDASK
                     FSTTI"
     gene            4352..4777
                     /locus_tag="VFV99_31235"
     CDS             4352..4777
                     /locus_tag="VFV99_31235"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5063909.1"
                     /translation="MKTTPSRVLEQLIAQHDELRAIMDRCEELADRLDAGEGNPLALT
                     REIAKLRLAFDAHNKFEEQLLRPVLHEMDAFGDVRIDRMFSEHVDEHRSMRQQLGDPT
                     TNALRGVIDSLRVHLQAEERYFLSSTVLRDDVVSVESGG"
     gene            4807..5232
                     /locus_tag="VFV99_31240"
     CDS             4807..5232
                     /locus_tag="VFV99_31240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5063910.1"
                     /translation="MRFKKILVGVDFSQPSREAMRAAAALAKEFGAELTVVHFWQIPL
                     IGAELPVQAAYLDEMRAAAEQELAAWTAEASSLADRKVTSVFEIGVPWNEMVKLLQRD
                     RYDLAVVGTHGRTGLKHVLLGSVAERVVRHAPCAVLVAR"
     gene            5505..7688
                     /gene="ccoN"
                     /locus_tag="VFV99_31245"
     CDS             5505..7688
                     /gene="ccoN"
                     /locus_tag="VFV99_31245"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX5063911.1"
                     /translation="MSNETVRYDDAIVRRFVTATIVWGAVGMLVGLWCALQLALPDLN
                     FGPYFTFGRLRPLHTNAVIFAFAGNGIFAAVYYSSQRLLKARPIDWLSKFHFWGWQTI
                     ILAAAITLPLGFTQGKEYAELEWPIDVAITIVWVGFATNFFAMVRNRREKHLYVALWF
                     YIASIVTIAILHIFNNLVIPAGLFKSYSLYAGVQDAFMQWWYGHNAVAFVLTTPFLGL
                     MYYFLPKAAERPVFSYRLSILHFWSLVFIYIWAGPHHLHYTALPQWASTLGMIFSVML
                     WMPSWGGMINGLLTLRGAWNRVAEDPVLKFFVVGITFYGMSTFEGPMLSVKSVNALSH
                     YTDWNIAHVHGGALGWVGFMVFGMVYWLAPRLYQTKLYSKKLATTHFWVATIGIVIYV
                     IAMWASGITQGLMWRAFDETGRLQYPDFVESVTRIVPMYWMRALGGSLYITGLLIGAY
                     NLFKTWQSRPAKYAEIEVEAPPMVRAKKIAERDKRVLERAAESPQHWHRTWEGLPAFF
                     TIMTAAAVIVASLFEILPTFLIGGNVPTISTVKPYTPLELYGRDIYLREGCYNCHSQM
                     VRPFLDETVRYGQNGEPAEYSRAGEFVYDHPFQWGSKRTGPDLAREGARRDELWHLRH
                     FENPRSTSPGSVMPNYPHLLTDTIPWDVIQDRVDAMAMLGVPYDATALNHAASEAQHQ
                     AEHYAKELATEGGPPNMQNKEVIALIAYMQRLGVDIRLSKQGGTK"
     gene            7685..7909
                     /locus_tag="VFV99_31250"
     CDS             7685..7909
                     /locus_tag="VFV99_31250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063912.1"
                     /translation="MIRLSELVSALSPTHFAQLALILFMAVFVAVAIRHGGKRRAAEH
                     AQCAQLPLADDAGQAGLVRSMTRGDAGERK"
     gene            7906..8523
                     /locus_tag="VFV99_31255"
     CDS             7906..8523
                     /locus_tag="VFV99_31255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5063913.1"
                     /translation="MTPPSIAGTAEAGVPEKTDEPRVLEHAYDGIREYDNPLPGWWRA
                     IFWMTIVFAAGYWVWFHVGGWGNMPDAKYRVALAEYTEQKGQREAAEARDVSEESLAR
                     MAHDPKAVEHGQAIFASRCVSCHTEDGHGLIGPNLTDRYQLHGTTRMDIFKTIRGGVS
                     GTAMLAWGEQMPASDIVNVAAFVTTLRGKDIPGKEPQGQPVEPFK"
     gene            8520..9884
                     /gene="ccoG"
                     /locus_tag="VFV99_31260"
     CDS             8520..9884
                     /gene="ccoG"
                     /locus_tag="VFV99_31260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX5063914.1"
                     /translation="MTHNVPTDRVLSTLNQDGTRNYIRPKLAHGRFLTRRRVVGFLLI
                     ALFVLLPRLRIHGHPALLIDLVTREIHVFGALFRPSEGFVLALLGITIVIAVFLVTAL
                     FGRVWCGWGCPQTVYLELVFRPIERWLEGSRGQRASPLRMVIKWTLYSAVAFALSNVF
                     LAYFVGTDRLEQWVFGSPLDHPIGFTIVIAVAALMLLDFGWFREQLCTIACPYGRLQS
                     VLLDKQSLIVGYDVGRGEPRTKPKKKLPVVGGDCVDCSACVSVCPTGIDIRDGLQMEC
                     IGCAQCIDACDAVMDKLNRKRGLIRYTSQDELAGKPRRVWRMRTIIYPLLLLVAGGML
                     AWSVEARESTEVWIERVQGPSFVELPDGKISAQARIKLENQGDSERRYHLLLVESPGA
                     SLRSQPMWTIKPHKSLEIPIFIDVPRESFVDGKRRVFLRIHDSGGFERVVTLTLLGPV
                     GATR"
     gene            9881..10060
                     /locus_tag="VFV99_31265"
     CDS             9881..10060
                     /locus_tag="VFV99_31265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063915.1"
                     /translation="MSAKHKWIAAIVGLLVANVLAMVFLIVVANGEGHSKVLPSYKGV
                     IEKTVVEKTGGVEKK"
     gene            complement(10213..10344)
                     /locus_tag="VFV99_31270"
     CDS             complement(10213..10344)
                     /locus_tag="VFV99_31270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063916.1"
                     /translation="MNELRDTDGRNQTSREQRCRTLDVADPTREIDRATDRARNRTE"
     gene            10582..11001
                     /locus_tag="VFV99_31275"
     CDS             10582..11001
                     /locus_tag="VFV99_31275"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5063917.1"
                     /translation="MGSAVAASADGSKRSAFTSVLVRIRPKSAAKRSYLIGTLTGLLP
                     CGWLWAFVIAAAGTGSIAGGAAAMFAFWLGTVPAMVGLLRVTGPVLARIRARMPAITA
                     VALIVLGFGTLALRWRDAGITQVDKPHCHCHDHGASS"
     gene            10998..>12665
                     /locus_tag="VFV99_31280"
     CDS             10998..>12665
                     /locus_tag="VFV99_31280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEX5063918.1"
                     /translation="MTAALAHACAHCSLPIPAGRGGDFCCAGCEVVHGAIAEYGLAKF
                     YALRAPVDAKPANTTTRSYRELDDPAFQRLHVRTDTGGRAHAALYVEDLRCTACVWLI
                     EATPRCVDGVETVRVDLGRSRADVTWDPSTTSLAAVARHLDRIGHGVHPYRGLDRDAQ
                     RRREDRVLLVKLGVAGAAVGNLMLLAIALYCGLFQGMNAADTTFFRWASMLVAVPTLG
                     YAATPMFRTALGALRARRLHLDLPLSIGILAGLVWGTANVVRGVGEIYFDSLAMLVFL
                     LLVARWIVLRHQRRASTAAELLLALTPSRARRVTPTGIDDVPIEAIAEGDVVEVRATE
                     TIPVDGTIARGASTIDSGLLTGESRPVDVDVGATVHAGTVNLLAPIQIVAVRVGEQTR
                     VGALVASIEQLSARKAPIERLVDRIAGRFVPVVTVAAVVTFIVWSLLASPTVGAEHAM
                     ALLIVTCPCALALATPLAVTVALGRAARRGILIKGADALERLATPGTMFVDKTGTLTE
                     GKLAVASWYGDVDARALAAAVEAGSAHLIARAIAAAATPAAAATDIQE"
BASE COUNT         2106 a         4024 c         4204 g         2331 t
ORIGIN      
        1 gtcgactgcg acaacccgac atccaccttc ctggacagcc gaccaccggg ccgatcaaca
       61 cgaacgatgt gccgggacct caagccccgg acgggacagg tctgcgcttg acgcgaccgc
      121 cactacatag tcgttccatc tcctggcggc gtctggccgc cgacggatcg gtgccgacgg
      181 ggatggcgat gaatcgaccg atcgactcac tgcgcgaggc agctaggcgc gctgcggaac
      241 ggtgacgtcg aagcgagtgc cgctcgtcga cgtatcgatc tcgatgcggc cgccgtgcat
      301 ggagacgagg ctgtgcacga tcgccatgcc catgcccgtg ccggtctcct tcgtcgagaa
      361 gaacggctcg tagatccgtt gcagcacctc aggcgggatg ccgctgcctt cgtcggtgac
      421 gcggatgtgg accgagttct cgccttcgtt cgtgatccac aggccgacgt gtccgccata
      481 cgtgctggct tcgaggccgt tgcggacgag gttctggatg acctgatgga tcttgcccga
      541 gtcgacctgc gcgatgatcg gcggcacttc gtggaacgtg agctcgacgc cgcgctgttg
      601 tgcgagtgga cgttcgagct cgatcacgtg gcggccgatc tcgatgaggt cgtgctcgtg
      661 caagtgcagc tcgggcggac gcgcgaacga caggaagtcg ttgagaaggt tcgagaggcg
      721 ttcgagctcc tggttcgcga gcgttgtcgg ctcgatgagc ttgctgtcat cgccgacctg
      781 gcggcggatg cggcgctcga gtagctcgag ctgaaggcgc gcggcgttga gtgggttgcg
      841 aacttcgtgc gcgaggccgg ccgagagcgt ctgcatcgcg gtgaggcgat cggattggat
      901 acggcgctcg cgttgcagca gcttctcttc ggagtcgagc tgatagtggc gcagcatgac
      961 tgcgagctcg atgtcgagga gcttgttgac ggcgcggatc accgtgcagg cgcgatcgag
     1021 cgggtagagc tcgacgatct tctcgaggta cgcggtgcgc accacgttca tcgccgtgaa
     1081 catgtactgc tgcgcgaggc cgatctggac gtgccggcga ccgacgcgcg agcgcttctc
     1141 gtagaacttg tcgtcgtagg gaccgagcag gccagtcgac atccagtcga tcagcgagcc
     1201 gcgaagtcgc tcgacctgtt cggggccgct gatgacggcg gctgcgccgg gatgctcgaa
     1261 gaccgcggcg tagaagcgct gcgcgatgct cgggaagtga ggctggagtt tcggatgcag
     1321 atcgcgcaat agctcgatct cgcgctcgcc gagctcgacg taacttatta cctcggcgag
     1381 aaactcactc actcgtgtga cctgtagcag ctacttgttg ctgccgctat ttgttccggt
     1441 gctgccactc gccaccaccg aaacctcgga gcgcggcgca gcgttgtagt cgtggaggcg
     1501 atactggatc gtacgaacgc tgatgccgag catgtcggcc gctttcgacg tcgagccacc
     1561 ggtggccttg agcgattcga gaatcgcgta gcgctcgatc tcggcgagcg acgaaccagg
     1621 aatcggtggc atgccggccg gacgcagcgt cgggcggacg ttcggtggca gattgcgcgg
     1681 ctcgacgatg gggccggtcg tgaggacgac ggcgcgctcg atcgcgttct cgagctcacg
     1741 aacgttgccc ggccagtcat agctcatgag gagctcgagc gtttgcggtg cgaacgtctc
     1801 gatcggcttg ccattgtcct tggcgtagcg atcgaggaag aacttcgcga gcgccgggat
     1861 gtcgctcttg cgatcgcgca gcggtggcat gtcgatcgtg acgacgttga gccgatagta
     1921 gaggtcttcg cggaagcgac cggccttgac ctcgtcggcg aggtgcttgt tcgtcgctgc
     1981 gatcacgcga acgtcgacct tgatcgtctg cgtgccgccg acgcgctcga actcgtgctc
     2041 ttggaggaag cggaggagct tgacctgaac cgacggcgag atttcgccga tctcgtcgag
     2101 gaatagcgtg ccgccgtcgg cgatcgagaa ccggccatct ttgcgcgcca tggcgccggt
     2161 gaacgcaccg cgttcgtggc cgaacagctc gctctcgagc agcgtctccg caagcgctgc
     2221 gcagtgcagc ttcacgaacg gaccgttcgc gcgcggcgag cgtgcgtgga ttgcgttagc
     2281 gacgagctcc ttaccggtac cggattcgcc ggtgatcaga acggtcgctt tgctcggcgc
     2341 gacttgatcg gtgatctcga acacccgttg catgggcggg ctcgagccaa tgatgttgct
     2401 cggtgccacg cggtcgcgaa cgcgcgcgcg caactggcgc gtttcgcgct ttagttgcag
     2461 gtgctccatc accttgtcga tgacgacaag cagctcgtcg aagttgatcg gcttggtcag
     2521 gtactccgca gcgccggcac gcattgcgtc cactgccgaa gcgaccgcac caaatgcggt
     2581 catcacgatc acggacggcg gatcctccat cgcgcgcagt ttcttcacga gctcgatgcc
     2641 gtccatgcca ggcatcttga gatccgtcac aacgatatgc ggcgtgaagg cttcgtactt
     2701 gccgagcgcc ttgaacgcgt cggccgcggt ttcgacctcg aaaccctcgt cccgcaacag
     2761 ctctgcgaga gccgtacggg cgttcacttc gtcatcaaca actaggatgc gaccagaccg
     2821 ggacatacca accccctcag ccagaatcgt gccacgcaaa tccggcagtt tggcgcatat
     2881 aggctgcaag agctgcgtct aacgacaagc ctagcgcaac agttgcgcga aaaaccgccg
     2941 aaaatgcggg cacgcaaact gctctagcgg cattcatgac cttgcccgct gagctaacgc
     3001 cggagtgttt ggcagagcaa tcgagccgtg ggcctcgcta tacgagctac ccacctgcga
     3061 ccgagttcgc accgatcgat accgcgcggg tcgtacgcga gctcgcgacg gtccagtgcg
     3121 aaggcgcgcc ggtatcgctc tacgtacacg tcccattttg tcggagcctg tgttggtact
     3181 gcggctgtaa cgtgatcccg tcacgcgata cgtcgcgcgg cgagcattac gtcgacgtgc
     3241 tcgcgaccga gctcgcgttg ctcgcgggtt cgctcggtgt tggatttccg gttgcggaga
     3301 tcgcgctcgg cggcggttcg ccgaattttc tgacgccgcg atcgctgcgg acgctcaaga
     3361 ccgcgatcga tcggtcgttc acggtgcaga agaccgcgcg gctgtcggtc gagctcgatc
     3421 cgcgcgatac gaccgcgacg cagatcgaca cgttcgcgga tgtcggcttt cgcgtgctct
     3481 ccgtcggcgt gcaggacttc tcgacgcgcg tccaggatgc gatccaccgc caccagacgc
     3541 tcgcgcagac cggctggttg atcgaacgcg cgcgtgcggc cggcttcacc gacgtcaacg
     3601 tcgacatggt gtacggcttg ccgcgtcagc acgagagctc gttccttgcg acgctcgagt
     3661 cggtgatcgg cctcgggccg gatcgcatcg cactgttcgg ctacgcacac ctgccggatc
     3721 gattgccgca tcagcggctc gtcgagaagg cggggcgggt actcgatcgg tacgagcgcg
     3781 cgaccctgct gctcctcgcg atcgagaagc tcaccaacgc cggctacatc catctcggcc
     3841 tcgatcactt cgcgaagccc ggctctgcac ttgcgcgtgc cgccgccgcc aaacgcatgg
     3901 tgcgcacgtt ccagggctac gtcgagcatc gcgcggacac gctgctcggg ctcggcacgt
     3961 cggcgatctc gtcgacgccg cggatgtact ggcaaaactt tggcgacctc gcgccatggg
     4021 aggacgcgat cggcgcgcag cggctgccgg tgcaacgcgg ggcggagctc gacgccgacg
     4081 atcgcatccg ccgcacgctg atcgagcgcc tgatgtgcga cggcgaagca gatctggatc
     4141 agctcgagtc gcggttcggc atcgcgccgc tcgactattt cgcacgcgag ctcgaacgac
     4201 tcgcggccga gccagcgctc gcgaacctgg atcggcacac gattcgcact accccactcg
     4261 ggaagctgtt ggtgcgcaac gtatgcatga tgttcgaccg ataccaccgc gaggacgcgt
     4321 cgaagttctc gacgacgatc taggagaggc cgtgaagacc accccgagta gagtcctcga
     4381 acaactgatc gctcagcacg acgagctgcg cgccatcatg gatcgctgcg aagagctcgc
     4441 cgaccggctc gatgccggcg agggcaaccc actcgcgctg acgcgcgaaa tcgcgaagtt
     4501 gcggctcgca ttcgacgcgc acaacaagtt cgaagagcag ctgctcaggc ccgtgctgca
     4561 cgagatggat gcgttcggcg acgttcgcat cgaccgcatg ttcagcgagc acgtcgacga
     4621 gcaccgctcg atgcgccagc agctcggcga tccgaccacg aatgctttgc gcggcgtgat
     4681 cgacagtttg cgcgttcatc tccaggccga ggaacgctac ttcttgtcgt cgacggtgtt
     4741 gcgcgacgac gtcgtctccg tcgagagcgg cggctaactg gcgcgtgcga tgcaccaagt
     4801 cttcgcatgc ggttcaagaa gatcttggtc ggtgtcgatt tctcgcaacc gtcacgtgaa
     4861 gcgatgcgag ccgcggccgc gttggccaag gaattcggcg cagagctgac ggtcgtgcac
     4921 ttctggcaga tcccgttgat cggcgccgag ttgccggttc aagcggcgta tctcgacgag
     4981 atgcgtgccg ccgccgagca agagctcgct gcatggaccg cggaagcgag cagcctcgcc
     5041 gatcgcaagg tcaccagcgt gttcgagatc ggcgtgccgt ggaacgagat ggtgaagcta
     5101 ctgcaacgcg atcgctacga cctcgcggtc gtcggcacgc atggccggac cgggctcaag
     5161 catgtgttgc tcggctccgt cgcagaacgc gtggttcgtc acgcgccgtg cgctgtgctc
     5221 gtcgctcgct aatgcatcat cacgacgacc atcgccatga tgagcccggc tgcgatcgtc
     5281 gtccacatga tgatgtgcaa cggaatggtc cacgcctcgt cggatttcat gccacttgcg
     5341 tcgagcatcg aacgtgccag caggatctcg gctacggcgc gaccaagatg cgcatagcct
     5401 gcaccaccgg tctcgggcaa cgcatgcgct gcgcagatgc agtgctgtac accgcgtaga
     5461 cgccaccatg tgcacggcac gcgggttgcg aaaggactgg cgcgatgtcg aacgagaccg
     5521 tccgctacga cgatgcgatc gtccgccgct tcgtcactgc gaccatcgtg tggggcgcag
     5581 tcggcatgct ggtcggactg tggtgcgcgc tgcaactcgc gttaccggat ctgaacttcg
     5641 gtccgtactt cacgttcgga cggctgcggc cgcttcacac gaacgccgtg atcttcgcat
     5701 tcgcaggcaa cggcatcttc gcagcggtct attactcgtc gcagcgcctg ctcaaagcgc
     5761 gaccgatcga ttggttgtcc aagttccact tctggggctg gcaaacgatc attctcgccg
     5821 cagcgatcac gctgccgctc ggcttcacgc agggcaagga atacgccgag ctcgagtggc
     5881 cgatcgacgt cgcgatcacg atcgtgtggg tcgggttcgc gacgaacttc ttcgcgatgg
     5941 ttcgcaatcg gcgcgagaag cacctctatg tcgcgctgtg gttctacatc gcgtcgatcg
     6001 tgacgatcgc gatcctccac atcttcaaca acctcgtcat cccggcgggc ctgttcaaga
     6061 gctactcgct ctacgcgggc gttcaggacg cgttcatgca gtggtggtac ggccacaacg
     6121 cggtcgcgtt cgtgctgacg acgccgttcc tcggcctcat gtactacttc ttgccgaagg
     6181 cagcggagcg gccggtgttc agttaccggc tatcgattct ccacttctgg tcgctcgtct
     6241 tcatctacat ctgggcgggc ccacaccacc tgcactacac cgcgttgccg cagtgggcct
     6301 cgacgctcgg catgatcttc agcgtcatgc tgtggatgcc gtcgtggggc ggcatgatca
     6361 acggcctgct cacgctgcgc ggtgcgtgga atcgcgtcgc cgaggatccg gtcctgaagt
     6421 tcttcgtcgt cggcatcacg ttctacggca tgtcgacgtt cgaaggaccg atgctgtcgg
     6481 tgaagagcgt caacgcgctc tcgcactaca ccgactggaa catcgcgcac gtccacggcg
     6541 gcgcgctcgg ctgggtcggt ttcatggtgt tcggcatggt gtactggctc gcaccgcgcc
     6601 tctatcagac gaagctgtac tcgaagaagc tcgcgacgac gcacttctgg gtcgcgacga
     6661 tcggcatcgt catctacgtc atcgcgatgt gggcgtcggg catcacgcag ggcctgatgt
     6721 ggcgcgcgtt cgacgagacc ggccgtcttc agtacccgga cttcgtcgag agtgtgacgc
     6781 gcatcgtgcc gatgtactgg atgcgcgcac tcggtggctc actctacatc acggggttac
     6841 tgatcggcgc gtacaacctc ttcaagacct ggcagtcgcg gccggcgaag tacgcggaga
     6901 tcgaagtcga agcgccgccg atggtgcgcg cgaagaagat agcagagcgc gacaagcgcg
     6961 tgctcgagcg cgcagctgaa tcgccgcagc actggcaccg cacgtgggaa ggcctgccgg
     7021 cgttcttcac gatcatgacc gcggcggcgg tgatcgtcgc ctcgctgttc gagatcctgc
     7081 cgacattcct gatcggcggc aacgtgccga cgatcagcac ggtcaaaccg tacacaccgc
     7141 tcgagctcta cggccgcgac atctacctgc gcgagggttg ttacaactgc cactcgcaga
     7201 tggtccggcc gtttctcgac gagacggtgc gctacggcca gaacggcgag ccggcggagt
     7261 acagccgcgc cggcgagttt gtctacgacc atccattcca gtggggcagc aagcgcaccg
     7321 gtccggatct cgcgcgtgaa ggcgcccgcc gcgacgagct ttggcacctg cgtcacttcg
     7381 agaatccgcg ctcgacgtca cccggctcgg tgatgccgaa ctatccgcac ctgcttaccg
     7441 acacgatccc gtgggacgtg atccaggacc gcgtcgatgc gatggcgatg ctcggtgtcc
     7501 cgtatgacgc gacggcgctg aaccacgcag cgagcgaggc gcagcaccag gccgagcact
     7561 acgcgaaaga gctcgcgacc gaaggtggtc caccgaacat gcagaacaaa gaagtcatcg
     7621 cgctcatcgc gtacatgcag cggctcggcg tcgatatccg cctgagcaag caaggaggta
     7681 ccaagtgatc cggctatccg agctcgtgtc ggcattgtcg ccgacgcatt tcgcccagct
     7741 cgcactcatc ttgttcatgg cggtgttcgt cgcggtcgcg atccgtcacg gcggcaagcg
     7801 tcgcgcggcc gagcacgcgc agtgcgcaca gcttccgctc gcagacgacg ccggccaggc
     7861 cggcctcgtc cgatcgatga cacggggcga cgcgggagaa cgcaaatgac gccgcccagc
     7921 attgccggca ccgccgaagc gggtgttcca gagaagaccg acgagccgcg cgtcctcgag
     7981 cacgcctacg acggcattcg cgagtacgac aacccgctgc ctggttggtg gcgcgcaatc
     8041 ttctggatga cgatcgtgtt cgccgccggt tattgggtct ggttccacgt cggtggctgg
     8101 ggcaacatgc ccgacgcgaa gtatcgggtc gcgctcgccg agtacaccga gcagaagggt
     8161 cagcgcgaag cggctgaagc ccgcgacgtc agtgaggagt cgctcgcccg catggcgcat
     8221 gacccgaagg cggtcgagca cggccaggcg atcttcgcgt cgaggtgcgt gagctgccac
     8281 accgaggatg gtcacggcct gatcggcccg aacctcaccg atcgctatca gctccacggc
     8341 acgacgcgca tggacatctt caagaccatt cgcgggggtg tctcgggcac cgcgatgctc
     8401 gcgtggggcg agcagatgcc cgcgtccgac atcgtcaacg tcgccgcgtt cgtgaccacg
     8461 ctgcgcggca aagacatccc cggcaaagag ccgcaaggcc agcccgtgga gccgttcaaa
     8521 tgacccacaa cgtcccgacc gatcgcgtgc tgtccacgct caaccaggac gggacgcgca
     8581 attacattcg gccaaagctc gcgcacgggc ggtttctcac gcggcggcgc gttgtcggct
     8641 ttctgctgat cgcgctgttc gtgctgttgc cgcggctgcg cattcacggt catccggcgc
     8701 tgctgatcga tctcgtcacg cgcgagatcc acgtgttcgg cgcactgttc aggccgagcg
     8761 agggcttcgt gctcgcgcta ctcggcatca cgatcgtgat cgcggtgttt ctcgtcaccg
     8821 cgctgttcgg tcgcgtgtgg tgcggttggg gctgcccgca gaccgtgtac ctcgagctcg
     8881 tgttccgtcc gatcgaacgc tggctcgaag gcagccgcgg tcagcgcgcg agtccgctgc
     8941 gcatggtgat caagtggaca ctgtattcgg ccgtcgcgtt tgcgctgtcg aacgtattcc
     9001 tcgcgtactt cgtcggcacc gatcgactgg agcagtgggt gttcggctcg ccgctcgacc
     9061 atccgatcgg cttcacgatc gtcatcgcgg tcgcggcgtt gatgctgctc gacttcggct
     9121 ggttccgcga acagctctgc acgatcgcgt gcccctatgg gcgactgcaa tcggtcctgc
     9181 tcgacaagca gtcgctgatc gtcggttacg acgtcggacg cggcgagccg cgtacgaaac
     9241 cgaagaagaa attgcctgtg gtgggcggtg actgcgtcga ttgttcggcg tgcgtcagcg
     9301 tctgcccgac cggcatcgac attcgcgatg gcctgcaaat ggagtgcatc ggctgcgcgc
     9361 aatgcatcga tgcgtgtgac gccgtgatgg acaagttgaa ccgcaagcgc ggcctgattc
     9421 gctacacgtc gcaggacgag ctcgccggta aaccgcgccg cgtgtggcgg atgcgcacga
     9481 tcatctatcc actgctgctc cttgtcgcgg gcggcatgct cgcgtggtcc gtggaagcgc
     9541 gcgagagcac cgaggtgtgg atcgaacgcg tgcaaggacc gagcttcgtc gagctgcctg
     9601 acggcaagat ctcggcgcaa gcgcggatca agctcgagaa tcagggcgac agcgagcggc
     9661 gctatcacct gctgctcgtc gaatcgccgg gggcatccct gcgcagccag ccgatgtgga
     9721 cgatcaagcc gcacaagtcg ctggagattc cgatcttcat cgacgtgccg cgtgagtctt
     9781 tcgtcgacgg caagcgccgc gtgtttcttc gtatccacga cagcggtggg ttcgaacgcg
     9841 tcgtgacgct gacgctgctc ggacccgtag gagcgacgcg atgagtgcaa agcacaaatg
     9901 gatcgccgcg atcgtcggcc tcttggtcgc aaacgtcctc gcgatggtct tcctgatcgt
     9961 ggtcgcgaac ggcgagggcc actcgaaggt gctgcctagc tacaagggcg tgatcgagaa
    10021 gacggtcgtg gagaagacag gcggcgtgga gaagaaatga ccttcgcgtt gctcgcaggc
    10081 atcgtggtga ccagcgtcgt cggtagcgtg cattgcctcg cgatgtgcgg gccgctcgtc
    10141 ggtcttcacg gcggcgcggc gaccacgcgg ctcgcgatca ctcactcgct cggccgtctg
    10201 acgacgtacg tgctattcgg tgcgattgcg ggcgcgatcg gtagcgcggt cgatctcgcg
    10261 ggtcgggtcg gcaacatcca gcgtgcggca acgctgctcg cggctggttt gattgcgacc
    10321 gtcggtatcg cgcagctcgt tcatgcgtac gttcgtcgtg ctagagcccg aagggacgcc
    10381 cgaggtcata tgcaactcag ttgcatatcg tctcgaacgc ggaacgattc gagcatacga
    10441 aggctggcgt tcgccacggc actcgtccag gccgacgtga acctgcgccg atcgacgagc
    10501 accgcggaac cggttcagcc ggccgctgcg agtgctgtcg ggctgaacga ctccgcgctt
    10561 gcggcgaggg ctgtcggacc tatgggctcg gcggtcgcgg cgagtgccga cggatcgaag
    10621 cgctcggcgt tcacatcggt gctggtgcga atccggccga agagtgcggc gaagcgctcc
    10681 tacttgatcg gcacgcttac ggggctcctt ccgtgcggtt ggctgtgggc gttcgtcatc
    10741 gcagcggcgg gaacgggctc tatcgcgggc ggtgctgccg cgatgttcgc gttctggctg
    10801 ggaacggttc cggcgatggt gggcctactg cgggtgaccg gacccgtgct cgcgcggatt
    10861 cgcgcacgca tgccggcgat cactgccgtt gcgttgatcg tgctcgggtt tggcacgctg
    10921 gcactgcgct ggcgagacgc tggcatcacg caagtcgata aaccgcattg ccactgccat
    10981 gaccacggag cgtcgtcgtg acggccgcgc tcgcgcacgc ctgcgcgcac tgcagcttgc
    11041 cgatcccggc cgggcgcggc ggcgacttct gctgcgccgg ctgcgaggtc gtgcacggtg
    11101 cgatcgccga gtacgggctc gccaagttct acgcgttgcg cgcgccggtc gacgcgaaac
    11161 ccgcgaacac gaccacgcgg agctaccgcg agctcgatga cccggcgttc cagcgcctgc
    11221 acgtgcgcac ggacaccggg ggccgcgcgc atgccgcgct gtacgtcgag gatctgcgct
    11281 gcaccgcgtg cgtctggttg atcgaagcga cgccgcggtg cgtggacggt gtcgagacgg
    11341 tgcgcgtcga tctagggcgc agccgcgccg acgtcacctg ggatccgagc acgacctcgc
    11401 tcgctgccgt cgcgcggcac ctcgatcgta tcggccacgg cgtgcacccg tatcgcggcc
    11461 tcgatcgcga tgcgcaacgg cgccgcgaag atcgcgtcct gctcgtcaag ctcggtgtcg
    11521 ccggcgctgc cgtgggcaac ctgatgctgc tcgcgatcgc gctgtactgc ggcttgttcc
    11581 agggcatgaa cgcggcggac accaccttct tccggtgggc gtcgatgctc gtcgcggtgc
    11641 caacgctcgg ctacgccgca acgccgatgt tccgcaccgc gctcggtgcg ctgcgtgcgc
    11701 ggcggctcca cctcgatctg ccgctctcga tcggaattct cgccggcctc gtgtggggga
    11761 ccgcaaacgt ggtgcgcggg gtcggcgaaa tctatttcga cagcctcgcg atgctcgtgt
    11821 ttctgctgct cgtcgcgcgc tggatcgtgc tgcgccatca gcgtcgcgcc tcgacggcgg
    11881 ccgagctgct gctcgcgctg acgccgagtc gtgcgcgccg agtcacgccg accggtatcg
    11941 atgacgtgcc gatcgaagcg atcgccgagg gtgacgtcgt cgaggtgcga gcgaccgaga
    12001 cgattccggt cgatggcacg atcgcgcgcg gcgcatcgac gatcgacagc ggtctgctca
    12061 ccggtgagtc gcggcccgtc gatgtcgacg tcggcgcgac cgtacatgcg ggcacggtga
    12121 acctgttggc accgatccag atcgtggcgg ttcgcgtcgg cgagcagacg cgcgtcggtg
    12181 cgctcgtcgc gagcatcgag caactgtcag cgcgcaaggc gccgatcgag cggcttgtcg
    12241 atcggatcgc gggccgattc gtgccggtcg tcaccgtcgc agcggtcgtg acgttcatcg
    12301 tgtggtcgct gctcgcgtca cccacggtcg gcgccgagca cgcgatggcg ctcttgatcg
    12361 tgacgtgccc gtgcgcgctc gcgctcgcga caccgctggc cgtcacggtc gcgctcggcc
    12421 gcgccgcgcg tcgcggcatc ttgatcaaag gtgcagatgc gctcgagcgg ttggcgacac
    12481 ccggcacgat gttcgtcgac aagactggca cactcaccga gggcaagctc gcggttgcat
    12541 cgtggtacgg cgatgtcgat gcgcgtgcgc tcgcggcggc ggtcgaggct ggtagtgcgc
    12601 atctgatcgc gcgtgcgatc gcggctgcgg cgacaccggc ggcagcggca accgacatcc
    12661 aggag
//
LOCUS       DASUFX010000774         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_959555,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000774 DASUFX010000000
VERSION     DASUFX010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959555"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1432
                     /locus_tag="VFV99_31285"
     CDS             164..1432
                     /locus_tag="VFV99_31285"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5063919.1"
                     /translation="MTSSIELTKLIRGVGQAARAAARIVAKAPAEQRTRALAAIATAI
                     DAQRAEILAANAKDVAAAEAAKTAPAMVDRLRLDDKRIAALVRAVNEIADAPEVLGRV
                     EKVETRPNGLEVSRVRIPLGVIAMIYEARPNVTVDAAALCLKAGNACILRGGREALES
                     NRTLLRAVQDGLAAAGLPSEAVQLVPITDREAIAELVKLDELIDLCIPRGGEGLIRYV
                     ADHARVPVIKHYKGVCHVYVHAAADLALAAKIVENAKVSRPGVCNALETVLVDRAIAE
                     QFIPMLVTTLSPRVELRGDDTVRKLGGTSVNIATDDDWHAEYLDLILAVRVVDGIDAA
                     IEHIERYGSNHTESIITRDQTAADKFLREVDSSTVMVNASTRFADGGELGLGAEIGIS
                     TTRLHAYGPMGAEGLTTTKFVVRGSGQIRT"
     gene            1571..1948
                     /gene="rsfS"
                     /locus_tag="VFV99_31290"
     CDS             1571..1948
                     /gene="rsfS"
                     /locus_tag="VFV99_31290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5063920.1"
                     /translation="MRALTLALDKKALEPVLLDVRHLCSFCNYQLIISGRSERQVDAI
                     ADAIELGLKQEDMRPISTEGARTGQWALLDYGDFVVHVFLHAAREHYDLEGLWNDAPR
                     VPIDVPADARLPPEETYETSAVP"
     gene            1945..2373
                     /locus_tag="VFV99_31295"
     CDS             1945..2373
                     /locus_tag="VFV99_31295"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5063921.1"
                     /translation="MKIFVGAIGRLKEPYFVAAEDEYRKRLRPYCTLTVHEAKDEAAL
                     LRALPDGAQLFVFDERGESMTSAQFAELLAAEAQHGKGAPLAFLIGGADGHSDAVRKR
                     AKKLIAFGKLTIAHRLVRILVLEQLYRAFKIVRGEPYHRD"
     gene            complement(2375..2506)
                     /locus_tag="VFV99_31300"
     CDS             complement(2375..2506)
                     /locus_tag="VFV99_31300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063922.1"
                     /translation="MSDPCPYAVRTQQGGYKHSERCTSASGCLLYIHGDYGFDVRPM"
     gene            2606..2875
                     /locus_tag="VFV99_31305"
     CDS             2606..2875
                     /locus_tag="VFV99_31305"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5063923.1"
                     /translation="MFVFDERGEAITSAQFAELLAAEAQHGKGAPLAFLIGGADGHSD
                     AVRKRAKKLIAFGKLTIAHRLVRILVLEQLYRAFKIVRGEPYHRD"
     gene            complement(2877..3608)
                     /locus_tag="VFV99_31310"
     CDS             complement(2877..3608)
                     /locus_tag="VFV99_31310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063924.1"
                     /translation="MASAAEILGVLDAESQSFSFPMLDNGYVYPAAARLSLYRSDLDW
                     AVVIEIFGYSPRAGVPTLDIGTFGSRLANRKPASNYVTEEAHRNYLRAHPHDEHCFLY
                     PIDDGPWIDEELVTDDPDGELTLRGQARRLPSRAALQGVGIELADPNRIHIFELCRYL
                     AVEYRDGVLATPSERCVNVVPGTQLILQLEDWHHPDLVKSQWPSHVESFRQLAEVLAT
                     GDTSRYQPSLPPNTHWKHWPDGGTL"
     gene            complement(3629..>4293)
                     /locus_tag="VFV99_31315"
     CDS             complement(3629..>4293)
                     /locus_tag="VFV99_31315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5063925.1"
                     /translation="QPAAATSHGDPAPHRAPTVIAADKLIWQGKAEATKMAVLWTVGE
                     DVGRLLMLPAKSRGRPSTDLHDRVAIQLDAKPHVELIAMGGPPQDGIECHAAAPCTFY
                     EHTIPRGQQSAGSLAWEPYGPDRQFAPAWGTVETGSFGVFVELKPSTATFWHMHGHDV
                     RMVVLAGTVEYAQSGDPAFTLTPGSYVSQQGGYKHSERCTSASGCVLYIHGDYGFDVR
                     PM"
BASE COUNT          789 a         1406 c         1387 g          711 t
ORIGIN      
        1 tgtccgtcgt cacgcgtgac ggcgcggagt tggcccgcgg gctcgccgcc taccccggag
       61 atgatctgcg caagatccag ggcttgcatt cggccggtat cgaggccagg ctgggataca
      121 agtccatcga cgaagccatt caccgggatg atctcgtaat cttgtgacct cctcgatcga
      181 gctaaccaag ctgattcgag gcgtgggcca ggcggcgcgc gcagcggcca ggatcgtcgc
      241 gaaggccccc gcagagcagc gcacgcgggc actcgcagcg atcgccaccg cgatcgatgc
      301 ccagcgcgcc gagatcctgg cagcgaatgc gaaggatgtc gcggcggccg aagccgcgaa
      361 gaccgcaccc gcgatggtcg accggctacg cctcgacgac aagcgcatcg ccgcgctcgt
      421 ccgtgcggtg aacgagatcg ccgatgcgcc cgaggtgctc ggccgcgtcg agaaggtcga
      481 gacgcgcccg aatggactcg aggtgtcgcg cgttcgcatt ccgctcggcg tgatcgcgat
      541 gatctacgaa gcgcggccga acgtcaccgt cgacgccgca gcgctctgcc tcaaggcagg
      601 caacgcgtgc atcttgcgcg gcggtcgtga agcgctcgaa agcaaccgca cgctacttcg
      661 cgccgtgcag gatggcctcg cagcagccgg cctaccgagc gaagcagtgc agctcgtgcc
      721 gatcaccgat cgcgaagcga ttgccgagct ggtcaagctc gacgagttga tcgatctctg
      781 catcccgcgc ggcggcgaag gcctgatccg ctatgtcgcc gaccacgcac gcgtgccggt
      841 gatcaagcac tacaagggcg tctgccacgt ctacgtccac gcggctgccg atctcgcact
      901 ggccgcgaag atcgtcgaaa acgcgaaggt gtcgcggcct ggtgtctgca acgctctcga
      961 gacggtgctc gtcgatcgcg caattgcgga acaattcatc cccatgctcg tgacgacgct
     1021 gtcaccgcga gtcgagctgc gcggcgatga cacagtccgg aagctcggtg gtacatcggt
     1081 caacattgcg acggacgatg actggcacgc cgagtacctc gatctcattc ttgcagttcg
     1141 cgtggtcgac gggatcgacg ctgcgatcga gcatatcgag cgctacggtt ctaatcacac
     1201 cgagtcgatc atcacgcgcg atcaaaccgc tgccgataag ttcttgcgcg aagtcgattc
     1261 gtcgactgtc atggtcaacg cgtcgacccg cttcgccgac ggtggcgagc tcggcctcgg
     1321 tgcggaaatc ggcatctcga cgacgcgact gcacgcctac ggaccgatgg gagccgaagg
     1381 cctcaccacc accaagttcg tcgtccgcgg gagcggacag attcgtacgt agaggaggaa
     1441 cgagcgacgc atgaccaaag tcaaaaagag cgcccccaag gctgtacgat cgacaccgct
     1501 gtcgcgaggc agaagtagct cgccagcgtc gacaagccag ccgcaatcta ccgaaggcct
     1561 cgacggcgcg atgcgcgcgc taacgctggc actggacaag aaggcgctcg agccggtctt
     1621 gctcgacgtc cgtcatctct gttcgttctg caattaccag ctgatcatct ccggacgcag
     1681 cgaacgccag gtcgacgcga tcgccgatgc gatcgagctc ggcctcaagc aagaggacat
     1741 gcgaccgatc agcaccgaag gtgcgcgcac cggccaatgg gcgctgctcg actacggtga
     1801 cttcgtcgtc cacgtcttcc tgcacgccgc gcgcgagcac tacgatctcg aaggcctgtg
     1861 gaacgacgcg ccgcgcgtgc cgatcgacgt gcccgccgac gcgcgcctgc cgcccgaaga
     1921 gacgtacgaa acgtccgccg ttccgtgaag atcttcgtcg gcgcgatcgg caggctgaaa
     1981 gagccatact tcgtcgccgc cgaagacgag tatcgcaagc gcctgcgtcc gtactgcacg
     2041 ctcaccgtgc acgaagcgaa agacgaggcg gcgctgctcc gtgccctacc cgacggcgcg
     2101 cagctgttcg tgttcgacga gcgcggcgag tcgatgacga gcgcacagtt cgccgagctg
     2161 ctcgccgccg aagcgcagca cggcaagggc gcgccactcg cgttcctgat cggcggcgcc
     2221 gacggtcata gcgacgcggt ccgcaagcgc gcgaagaagc tgatcgcgtt cggcaagctc
     2281 acgatcgcgc accgcctcgt gcgcattctc gtgctcgagc agctctaccg cgcattcaag
     2341 atcgtgcgcg gcgagccata ccaccgcgac tagctcacat cggccgaacg tcgaagccgt
     2401 agtcgccgtg gatatagagc aggcagccgc tcgccgacgt gcagcgctcg ctgtgcttgt
     2461 agccaccttg ctgcgtacgg acagcgtacg gacaggggtc agacatgcct tgtatgcatt
     2521 cgtcggacgg gctttcgagc aacgcataca agtcatgtct gaccccgtcc gcgatgtctg
     2581 accccgtccg tacgacggtg cgcagctgtt cgtgttcgac gagcgcggcg aagccatcac
     2641 gagcgcgcag tttgccgagt tgctcgcggc cgaagcgcag cacggcaagg gcgcgccact
     2701 cgcattcttg atcggcggcg ccgacggcca cagcgatgcg gtccgcaagc gcgcgaagaa
     2761 gttgatcgcg ttcggcaagc tcacgatcgc gcaccgcttg gtgcgtattc tcgtgctcga
     2821 gcagctctac cgcgcgttca agatcgtgcg cggcgagcca taccaccgcg actagatcag
     2881 agcgtgccgc cgtcgggcca gtgcttccaa tgcgtattcg gtggcagcga cggttggtac
     2941 cggctcgtgt cgccggtcgc gagaacctcc gctagttgcc gaaacgactc gacgtgactc
     3001 ggccattggc tcttcacgag atccgggtga tgccagtcct cgagctggag gatcagctgt
     3061 gtgccgggca cgacgttgac gcagcgttcg cttggcgtag cgaggacgcc atcgcgatac
     3121 tcgacagcca gatagcggca gagctcgaag atgtggatgc ggttcggatc cgccagctcg
     3181 ataccgactc cttgcagagc tgctcgtgac ggcagccggc gcgcctgccc gcgcaacgtc
     3241 agctcgccgt ccggatcatc ggtgacaagc tcctcgtcga tccacggtcc atcgtcgatc
     3301 ggatagagga agcagtgctc gtcatgtgga tgggcccgca ggtaattgcg atgcgcctct
     3361 tctgtcacgt agttgctcgc cggcttgcga ttggcgagcc ggctgccgaa tgtgccaatg
     3421 tccagcgtcg gtacgcctgc acgcggtgag taaccgaaga tctcgatcac gacggcccag
     3481 tcgagatcag agcggtacaa cgaaaggcgg gccgcggccg gatagacgta gccgttgtcg
     3541 agcatcggaa acgagaacga ttggctctct gcgtcgagga ctcccaggat ctcagcagca
     3601 cttgccatgt ccggcatgat tgcacggact acatcggccg gacgtcgaag ccgtagtcgc
     3661 cgtggatgta gagcacgcag ccgctcgccg acgtgcagcg ctcgctgtgc ttgtagccgc
     3721 cttgctgcga gacatacgag cccggcgtca gcgtgaatgc ggggtcaccg gactgggcgt
     3781 actcgacagt gccggcgagc acgaccatgc gcacgtcgtg gccgtgcatg tgccagaacg
     3841 tcgccgtgct gggcttgagc tcgacgaaca cgccgaacga gccggtctcg acggtgcccc
     3901 acgcgggcgc gaactggcga tcaggaccgt atggctccca cgcgagcgag cccgcggact
     3961 gctggccccg cggaatcgtg tgctcgtaga acgtgcacgg tgctgcagcg tggcactcga
     4021 tgccgtcctg cggtggccct cccatcgcga tcaactcgac gtgcggcttg gcgtcgagct
     4081 ggatcgcgac acgatcatgc aggtccgtcg aaggccgacc gcgcgacttc gctggcaaca
     4141 tgagcaggcg accgacgtct tcaccgacgg tccacagcac ggccatcttc gtggcctccg
     4201 ccttgccttg ccagatgagc ttgtccgccg cgatcaccgt cggcgcacga tgcggcgccg
     4261 gatcgccgtg gctggtcgca gcggcgggct gcg
//
LOCUS       DASUFX010000775         4616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_543076,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000775 DASUFX010000000
VERSION     DASUFX010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543076"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFV99_31320"
     CDS             <1..444
                     /locus_tag="VFV99_31320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063926.1"
                     /translation="ERETADRKLKDAVARADQAERAQKSLEAALEAAKTDAQKARDEA
                     GRAKAAAESKDKQLIEAAAGTPVPSGVSGAGEIATRAREVYEAINDILSEMRNNMVLV
                     QGELPNLQADGETMQAVTDAVEALVDSAETAKGALRGLRDLAETK"
     gene            445..2058
                     /locus_tag="VFV99_31325"
     CDS             445..2058
                     /locus_tag="VFV99_31325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5063927.1"
                     /translation="MTIEHKRSSKAEIEDLVKARYSLIWISSAEEQRVEEQLRKLCVE
                     REMRLEIWSITEGFKTVANGQGTRDVKDPMKAIDHVLRSEGRALFVLRDFHPFLKEPA
                     VVRRLRDAATELRKTKKSLLVLSPVTKIPPELEKSISAVLDWELPNRQEIEQAARKVL
                     PNLPQATQQVIESDPTFMERVVEGALGLTLVEAENVFAKSAVRTHTFDLETILEEKKQ
                     IIRKSGLLEYYEHREEFSDVGGMEVLKDWLVKRRNAFSSRARDFGLPLPKGILLIGVP
                     GTGKSLTAKAVGALWQMPLLRLDVGKIFGGLVGSSEENIRSVIRTCEAVAPAILWIDE
                     LEKGFSGTQSSGSTDGGTTSRVFASFITWLQEKTTPVFVIATANNVHQLPPELLRKGR
                     FDEIFFCDLPDREDRRQIMDIHLRKKNRDPGQFDMDKLIDATVDYSGAELEQAVIAAL
                     YDAFDSGNDLDTEGLLKTVTEIVPLAITMREMIDSMREWARTRARMASSRAKSPKKEG
                     WMARYGAPKAGLGDKGPEPGDDDTRKLEL"
     gene            2081..2488
                     /locus_tag="VFV99_31330"
     CDS             2081..2488
                     /locus_tag="VFV99_31330"
                     /inference="COORDINATES: protein motif:HMM:NF018565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HEX5063928.1"
                     /translation="MSHFTKCALKMTNLAALKKALADMQLKFTEAEQNQSVTVRGYRG
                     DKLQAAISIDMGRYDIGVIANQDGTYDITADWWGVETTKGVSEEEFKNTLSQKYQYHN
                     VKQACEEKGYAVEEEVNEEDGSIRLVVRKWVGE"
     gene            2485..2703
                     /locus_tag="VFV99_31335"
     CDS             2485..2703
                     /locus_tag="VFV99_31335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HEX5063929.1"
                     /translation="MRKQDIEIVINAKGEVTFQVKGVKGGSCIDETKFLEAALGGEAA
                     VVDQQKTGEYYEQSEGYVSTWSGEGKDE"
     gene            2722..3345
                     /locus_tag="VFV99_31340"
     CDS             2722..3345
                     /locus_tag="VFV99_31340"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX5063930.1"
                     /translation="MSDDQNDQNGQNQEELSDELARKYDPERLLKLVSRKAGRGEALD
                     ASIRSKYEKRFGVDLGHVRVVTGEFAEEFNKQRDAYAVTVGGTGMILMGNSPEKAAGT
                     RAGEALLAHELTHVAQAQRGLHNRGREVDFTHADEIEAHAVEHEVEQGHEGGQGAADE
                     TAKAAQAAMAAAEKQKEQIEKIKARVLEMMGEGARNQYLRNGVTRRP"
     gene            3374..4054
                     /locus_tag="VFV99_31345"
     CDS             3374..4054
                     /locus_tag="VFV99_31345"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX5063931.1"
                     /translation="MTDQSADAGVVQSRVQLERLVADDELAGRTIAGFAAPSVGLRSI
                     DLDGVTLDRLDLRGCDAEDTRLERAELKHSDLRTSQWKGALWHRVRAKDCDLTELDLS
                     GAQLMRCELGPVRMARVRFHKARIQDTTFKESELYSADFAGAMLLKCEFEGYEHATVS
                     LSRTDWTGATLFDVDFKRANMYGATLRGAVLVRCDLRSVNLCSADLTGARFVGCEMTG
                     ADLDGATF"
     gene            4063..>4616
                     /locus_tag="VFV99_31350"
     CDS             4063..>4616
                     /locus_tag="VFV99_31350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063932.1"
                     /translation="MPSRPDLRELAANVKEAFGEMDRETLLEILTFVVKEYVVEGPPP
                     MLVHQAETLADLQGLTFAQLIQALQTRLDHPELAMFAVDGDQVSVRVGGVMQPLLAAR
                     QPMTAQPAEQPRPQPGVRVVETSVQQRPAAPVPARTSGNAPPPARGLSVRGRPQGEAA
                     GEAPPQAQQPPQPPAAQGQQPPQG"
BASE COUNT          938 a         1391 c         1606 g          681 t
ORIGIN      
        1 gagcgcgaaa cggccgatcg aaagctgaag gatgcggtcg cgcgtgcgga ccaggccgag
       61 cgcgcacaga agagcctcga ggcggcgctc gaagcagcga agaccgacgc gcagaaggca
      121 cgcgacgaag cgggccgcgc gaaggcggcc gccgagtcga aagacaagca gctgatcgaa
      181 gctgcggcgg gcacgcccgt gccgtcggga gtctcaggtg ccggggagat cgcgactcgt
      241 gcgcgcgagg tctacgaggc gatcaacgac atcctgtcgg agatgcgcaa caacatggtc
      301 ctcgtgcagg gcgagctgcc gaatctccag gccgatggcg agacgatgca ggcggtgacg
      361 gatgccgtcg aagcgctcgt cgatagtgcc gagactgcga agggcgcatt gcgcggcctg
      421 cgcgatttag cggagacgaa gtgaatgacg atcgagcaca agcgcagcag taaggcggag
      481 atcgaagatc tggtgaaggc gcggtactcg ctgatctgga tctcgtcggc agaagagcaa
      541 cgcgtcgagg aacaactgcg taagctgtgt gtcgagcgcg agatgcggct cgagatctgg
      601 tcgatcaccg agggcttcaa gacggtcgcg aacgggcagg gcacgcgcga cgtcaaagac
      661 ccgatgaagg cgatcgatca cgtgctgcgc agcgaaggcc gcgcgttgtt cgtgctgcgc
      721 gacttccatc cgttcttgaa ggagccggcg gtcgtgcgcc ggctgcgcga tgcggcgacc
      781 gagctgcgca agacgaagaa gagtctgctc gtgctgtcgc cggtcacgaa gattccgccc
      841 gagctcgaga agagcatcag cgcggtgctc gattgggagc tgccgaatcg ccaggagatc
      901 gagcaggccg cgcgcaaggt gctgccgaac ctgccccagg cgacgcagca ggtgatcgag
      961 agcgatccga cgttcatgga gcgcgtcgtc gaaggcgcgc tcggcctcac gctcgtcgag
     1021 gcggagaacg tgttcgcgaa gagcgcggtg cgcacgcaca cgttcgatct cgagaccatc
     1081 ctcgaggaga agaagcagat cattcgcaag agcggtctgc tcgagtacta cgagcaccgc
     1141 gaagagttct ccgacgtcgg cggcatggaa gtcctcaagg actggctcgt caaacgtcgc
     1201 aatgcgttca gctcgcgtgc gcgcgatttc gggctgccgc tgccgaaagg catcttgctg
     1261 atcggtgtgc cgggtaccgg taagtcgctc acggcgaagg cagtcggcgc gctgtggcag
     1321 atgccgctgt tgcgtctcga cgtcggcaag atcttcggcg gcctcgtcgg tagctcggaa
     1381 gaaaacattc gctcggtgat ccgcacgtgt gaagcggtcg cgccggcgat cctgtggatc
     1441 gacgagctcg agaaaggctt ctcgggcacg cagtcgtcgg gctcgaccga cggcggcacg
     1501 acgtcgcgcg tgttcgcgtc gttcatcacg tggctgcagg agaagacgac gccggtgttc
     1561 gtgatcgcga ccgcgaacaa cgtccaccag ctgccgcccg aactgcttcg caaaggccgc
     1621 ttcgacgaga tcttcttctg tgatctgccc gaccgcgaag accggcgcca gatcatggac
     1681 atccatctgc gcaagaagaa ccgcgacccc ggtcagttcg acatggacaa gctgatcgac
     1741 gcgacggtcg actactcggg tgccgagctc gagcaggcgg tgatcgccgc gctctacgac
     1801 gcgttcgaca gcggcaacga cctcgacacc gaagggctgc tcaagaccgt caccgagatc
     1861 gtgccgctcg cgatcacgat gcgagagatg atcgactcga tgcgtgagtg ggcccgcacg
     1921 cgtgcccgca tggcgtcgtc gcgggcgaag tcgcccaaga aagagggctg gatggcgcgc
     1981 tacggcgccc cgaaagcggg tctcggcgac aaaggccccg agcccggcga cgacgacacg
     2041 cggaagctag agctgtagaa agccgtagaa cgggagactc atgtcgcatt tcaccaagtg
     2101 cgcgctcaag atgacgaacc tcgcggcgct caagaaagcg ctcgcggata tgcagctcaa
     2161 gttcaccgag gcggagcaga accagtcggt gaccgtgcgc ggctatcgcg gtgacaagct
     2221 gcaggctgcg atctcgatcg acatgggccg ctacgacatc ggcgtgatcg cgaaccagga
     2281 cggcacgtac gacatcacgg ccgactggtg gggcgtcgag acgacgaagg gcgtgtccga
     2341 ggaagaattc aagaacacgc tgtcgcagaa gtaccagtac cacaacgtca aacaggcctg
     2401 cgaagagaag ggctacgcgg tggaggaaga ggtgaacgag gaggatggct cgattcgctt
     2461 ggtcgttcga aagtgggtag gcgaatgagg aagcaagata tcgagatcgt catcaacgcg
     2521 aagggcgagg tgacgtttca ggtcaaaggc gtgaagggcg gtagctgcat cgacgagacg
     2581 aagtttctgg aggccgcgct cggtggcgaa gcggccgtcg tcgaccagca gaagaccggc
     2641 gaatactacg agcaatccga gggttacgtg tcgacgtggt ctggggaagg caaggacgag
     2701 taacctcgta aaaggattcg cgtgtcggac gaccagaacg accagaacgg ccagaaccag
     2761 gaagagctct ccgacgagct cgcgcggaaa tacgatccag agcgcctgct caagctcgtc
     2821 tcgcgcaagg cgggccgtgg cgaagcgctc gacgcgtcga ttcgcagcaa gtacgagaag
     2881 cgattcggcg tcgacctcgg tcacgttcgc gtcgtgaccg gcgagttcgc cgaagagttc
     2941 aacaagcagc gcgatgccta cgccgtcacg gtcggcggca ccggcatgat cctgatgggc
     3001 aattcgcccg agaaggccgc gggcacgcgc gccggcgaag cactgctcgc ccacgagctg
     3061 acccacgtcg cgcaggcgca gcgcggtctg cacaatcgcg gtcgcgaggt cgacttcacg
     3121 catgccgacg aaatcgaggc acacgcggtc gagcacgagg tcgaacaagg ccacgaaggc
     3181 gggcagggcg ccgccgacga aactgcgaaa gccgcgcagg ccgccatggc cgcggccgag
     3241 aaacagaaag agcagatcga gaagatcaag gcgcgcgtcc tcgagatgat gggcgaaggc
     3301 gcgcgaaatc agtacctaag gaacggcgtt acgcgccgac cctgatcgcc cgtccacggg
     3361 gtatccttcg agcgtgacgg atcagagcgc cgacgcaggc gtcgtgcaat cgcgggtgca
     3421 gctcgagcgg ctggtcgccg acgacgagct cgccggtcgc acgattgccg gcttcgcggc
     3481 gccgtccgtc ggtttgcgtt cgatcgatct cgatggtgtc acgctcgacc gcctcgatct
     3541 gcgcggctgc gacgccgagg acactcgact cgagcgcgcc gagctgaagc atagcgatct
     3601 gcgcacgtcg cagtggaagg gtgcgctgtg gcaccgcgtg cgcgcgaagg attgtgatct
     3661 gaccgagctc gatctcagcg gcgctcagct catgcgttgc gagctcggcc cggtgcggat
     3721 ggcgcgcgtg cgcttccaca aggcacgcat ccaggacacg acgttcaaag agagcgagct
     3781 gtactcggcg gacttcgccg gcgccatgct gctcaagtgc gagtttgaag gctacgagca
     3841 cgcgacggtg tcgctgtcgc gcaccgactg gaccggcgcg acgctgttcg atgtcgattt
     3901 caagcgcgcc aacatgtacg gcgcgacgct gcgtggcgca gtgctcgtgc gctgcgatct
     3961 gcgcagcgtg aatctgtgta gtgcggatct gaccggtgcg cggttcgtcg gttgcgagat
     4021 gaccggcgct gacctcgacg gagcaacgtt ctaggagtcg tcatgccctc tcgtcccgac
     4081 ctacgcgagc tcgctgccaa cgtcaaagaa gcgtttggcg agatggatcg cgagacgctg
     4141 ctcgagatcc tgacgttcgt ggtcaaggag tacgtcgtcg agggaccgcc gccgatgctc
     4201 gtccatcaag cggagacgct cgccgacctg caaggcctca cgttcgcgca gctgatccag
     4261 gcgctgcaga cgcggctcga tcatccagag ctggcgatgt tcgccgtcga tggcgatcaa
     4321 gtgagcgtgc gcgtcggcgg cgtgatgcag ccgctgctcg ccgcgcgtca gccgatgacg
     4381 gcacaacctg ccgagcagcc gcggccgcag cctggcgtgc gcgtcgtcga gacgagcgtc
     4441 cagcagcgcc ctgctgcgcc ggtgcccgca cggacatccg gcaacgcgcc accgccggcg
     4501 cgcggccttt cggtacgcgg acgtccgcaa ggtgaagcgg ccggcgaagc gccgccgcaa
     4561 gcgcaacaac caccgcagcc gccggctgcg cagggtcagc aaccgccgca aggtca
//
LOCUS       DASUFX010000776        15035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1097976, whole genome shotgun sequence.
ACCESSION   DASUFX010000776 DASUFX010000000
VERSION     DASUFX010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097976"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFV99_31355"
     CDS             <1..634
                     /locus_tag="VFV99_31355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063933.1"
                     /translation="DFEVDPTDAKADGLPPTFDQNMVFTDALLTDSLAMSVDDVQAFF
                     ENNAYGTRSWLASYQAGGMTAAEMVVEAAVAHEINPIVLIARMQVETSLVSKTTAPSQ
                     RLLDRALGCGCPDGGTCNPSYSGLHRQLDCGARTLRRWYDASIDGSGEWLKGRTRRTL
                     DGRSVTPISHATATLYAYTPWVLVGSGGTWLAWNVTRKYVRHATTTNTIH"
     gene            735..2381
                     /locus_tag="VFV99_31360"
     CDS             735..2381
                     /locus_tag="VFV99_31360"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5063934.1"
                     /translation="MRPPGWLVAVLVGAFAGLVFAGVSTYDFVQHLDRQVHSVHCSFI
                     PGAGASAGATGCQAAMMSSYSSVFRTSIWGGIPISLPAMAVFAFILFYGVDLLLSRRK
                     DDPRATAFLALACALPALASVTMLTISLTKLGTVCKLCVAIYIASALCLTGAIIVWRR
                     AVRGSTESVGSAKNGASLEAQVESPPASNGFLGTMFGIGVGFVAVPLILYMMMAPDHG
                     KFIGTCGVLEKPDDTYGVMVPVHPSGKAQALEILDPLCPACKAFEARLESSGMLDDLN
                     RKAILFPLDTTCNWMITENTHPGACAVSEAVLCAGDKAPEVIKWAFEVQDSVREQTKA
                     DPTAAERIVKQRWPELAACVGSPEAKSRLNKSLRWAVSNNIRVLTPQLFIDNVKLCDE
                     DVDIGLDYALAKMLDGNLQSRVVHTAKPVEGTSPSTAPLPTYQSGQQAGETKLPATTP
                     QPATTPGETKPAETKPAETKPGETTPTEAKPAETKPADKPAETKPPEAKPAETKPAET
                     KPTETKPAETKPAEGPVTSPGGTDKPAMPAEEKPGNGGGQ"
     gene            2378..3064
                     /locus_tag="VFV99_31365"
     CDS             2378..3064
                     /locus_tag="VFV99_31365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063935.1"
                     /translation="MTPIRIGVLVVLLAIPAAATAKWISSADSRLQEMTANPAEAPAV
                     AAAADVGYCSPELKKILRRVLMSCGLVGGEAARGCQPVQAKNVATMSGNDFNALFRPM
                     KERGGIIQFDLDKSDLDPGDIELIDKVFSEQKGASWFFVVSRASPEGSVTHNRDLSKA
                     RAESVMGHLRERFKDPDLDKEVGLLWLGEEFAQLETEFCEWKRSTTGTTCSPEELNRS
                     AFVAWIDCQL"
     gene            3043..3615
                     /locus_tag="VFV99_31370"
     CDS             3043..3615
                     /locus_tag="VFV99_31370"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5063936.1"
                     /translation="MDRLSALIIVIALAGCDSGKQEPAPGRADTTKVAKKDVSTAAFC
                     DVEFKGTSGPKFTVPKLVGGDIAPSASTWRWINVWATWCRPCVEEMPRLARWREKLGA
                     KYELSFISVDENEADVVEFKKEHPDAPASPRLAEPAKQADWYVSLGLDGGAPVPIHIF
                     VEPSGHVRCARAGGVREQDLPAIEKLLAGQ"
     gene            3677..4030
                     /locus_tag="VFV99_31375"
     CDS             3677..4030
                     /locus_tag="VFV99_31375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063937.1"
                     /translation="MLLRVLPVLLMVTVAAADATSRVDVTGDYSSNWDDVKLVQDGNE
                     VHGTYVCCGGGTIEGHIYEGHVIRYHWRNGGGADGEGVWEIMSTDRLEGTWGSGQSDD
                     DGGRWDLVRKSSLAN"
     gene            complement(4027..5337)
                     /locus_tag="VFV99_31380"
     CDS             complement(4027..5337)
                     /locus_tag="VFV99_31380"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX5063938.1"
                     /translation="MKHYFLLALAIVAACKSKEGPPSPQPTPAPTKPTAPPPADAQET
                     AVQHDVKTDMPPAKEIASLVKGSNNFALELWAHAGTGNTAISPLSISTALAMAWAGAK
                     GTTGEQMQKTMRLEGGTDLLVSQFARIATALQDPKRPLTLRIANKLFVDAHYDLDPVY
                     MAITKNAFAAPVQPAEFQNDPDGVRTAINTWVEQQTEKRIKDLIPPRAIDRETRVVIV
                     NAIYFLANWAEQFEPTATHPADFFVDGTQKKQVPTMHKQDTFKLAKADGAAMLELPYE
                     GGSASMYILLPDKPDGLPALEKKLPATLRTLQSKLVDDTVMVSLPRFTIEPGEPMRLA
                     ETLKTLGMTDAFDRDNADFTGISNPTDPKDRLFIGEVLHKAFVKVDEKGTEAAAATAV
                     VMPRGAGMPPKATPFTADHPFLFLIVDRSSGLILFIGRVVEPKS"
     gene            complement(5337..5543)
                     /locus_tag="VFV99_31385"
     CDS             complement(5337..5543)
                     /locus_tag="VFV99_31385"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX5063939.1"
                     /translation="MSEELAERVEDLEIKLAFQDKLIRELDALVRTFGDRLDATEREL
                     KELKQSIRSPEPALGPANEPPPHY"
     gene            5581..6237
                     /gene="tpx"
                     /locus_tag="VFV99_31390"
     CDS             5581..6237
                     /gene="tpx"
                     /locus_tag="VFV99_31390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX5063940.1"
                     /translation="MLRHGLFVVMIALAACSHSTTENDVVEFLKAKPADQRAQIVSKA
                     DGRVERTDVVRRGDKPLTLVGPTLDVGDAMPDVTLVDGKLEPIKLAALKGKIVVLSVV
                     PSIDTRVCETQTHKISDAIAQMPAGVEVYTISRDLPFAQTRFAEEAMSKTKFASDYQA
                     HELGRGFGLEVKESGLLARSVWVIGTDGKIAYRELVANQGSEPDYDALLAAVKRVAGG
                     "
     gene            6292..7725
                     /locus_tag="VFV99_31395"
     CDS             6292..7725
                     /locus_tag="VFV99_31395"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5063941.1"
                     /translation="MARLDTDIAAIAKRVLEEQGFATDPPTVAGASPTIDPNLRDLRD
                     LPWSSIDNDESRDLDQIEYAEQLDGNRMRVLVAIADVAALVSRNSPVDKYAAQNTATV
                     YTGVRTFPMLPESLAMDRSSLLEDRDRLAVVTEIIVDGAGALDDSATKVYPARVRNHA
                     KLVYEHVGAWLEGDETAAPANMAIAEQLRLHAQVTSWLRERRIECGALELETIEARPI
                     ARDGRVVDLAVVHKNRARDLVEDLMIAVNSATARFLERHMSSSIRRVVRAPKRWDRIV
                     ELAKTYGIELPATPNHRALGAFVAERRKADGENFAELSLSIVKLLGPGEYVLQRAADP
                     DQGHFGLAVDDYMHSTAPNRRYPDLVTQRLLKAAAASTPSPYSDDDLSAIAAHCTERE
                     DAARKVERTMRKVIAASMLHKRVGETFDATVTGASPKGTYVRLRRPPAEGRVIVGERG
                     LDVGDKIKVRLLATDVQRGFIDFAATQ"
     gene            complement(8009..8320)
                     /locus_tag="VFV99_31400"
     CDS             complement(8009..8320)
                     /locus_tag="VFV99_31400"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5063942.1"
                     /translation="MNTNVAKCLLVSKVLVADGMMAEDEREFLANMMTKLGLSDAEKR
                     SVIELEGLDDAEKIVDQLSEDERRAIVELLVDAASADGRLSPHELATVKRVSAALKLN
                     S"
     gene            8348..9946
                     /locus_tag="VFV99_31405"
     CDS             8348..9946
                     /locus_tag="VFV99_31405"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5063943.1"
                     /translation="MLVTYLDAPDGVTLPARMPSPFGDAHPLARHAAAELRRELDAGL
                     AHRLGLHEDGKMFGVLVVEDRGRLGFLKAFSGMVRGQWLLDGFVGPTFDLDARDAFWP
                     AGECELAALGADIAGLDQQLAPLRAELELRVATHERELVEIRERHRANRAARHVDREL
                     HGASPELDQQSRRDTAERRKLDARHAAARAALEQRMQPLLDQRARIDGERTTRSRHFL
                     QRIHATYALANARGDTRSLADVFGATPPPGGAGDCAAPKLLAHAYRHGMKPIALAELW
                     CGASPATGGRNDGSFYPACRGKCGPILAHMLGGLDVEPSPVFGADAIDAREPLTLFED
                     RWLAIVVKPVGLLSVPGRGTLSDSVQTRLRARYPSATGPLVAHRLDLDTSGVLLVAKD
                     QDTYAALQRCFAERTIDKRYIAWLDGNVHADHGTIELPLRVDLDDRPRQIVDEKHGKA
                     AVTEWHVLARTTGRTRVALVPKTGRAHQLRVHAAHPRGIGVPIVGDRLYGRPDARLML
                     HAESIGFVHPHTHERLERHSPSPF"
     gene            9985..11019
                     /locus_tag="VFV99_31410"
     CDS             9985..11019
                     /locus_tag="VFV99_31410"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5063944.1"
                     /translation="MGPSKTIGIELWGGQTRGASNVATCYRPAPRVNLGKPMRQLFLG
                     AYGKFADLCREVDEPGLALLAVDESTGAPAGLVRLRARVGRHVAAIVGRHDQCDLYLS
                     EHASLALRHLAIVLDPVQSWRRNSTSVRYRVLDLRTSDGFTDENGKPMRGLRAEGPAF
                     IRCAGHALFVLPLGDPTDWPENPADAWSMLPERIYFDEMRSSPDGSLTNMPLNASRKS
                     MIFRTHGVRDTGTGESLIDRGDLAGTLDVIGATRSGRMTIGDRALRDGVLLGRYARCD
                     GAELLEDLSMSRVHMLLLHTDDALLAVDTCSRNGSHLPGHSATRVISVSAGTEIHLGK
                     HTRIRWRWTA"
     gene            11036..12253
                     /locus_tag="VFV99_31415"
     CDS             11036..12253
                     /locus_tag="VFV99_31415"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX5063945.1"
                     /translation="MRSAVYALVTALVLVALTSSAKAQPRPSDPQDYPVIELVTMGVG
                     SLIWERHGHIALCVRYADPREDACYNYGIGDFHHPIGMATGFFRGTHSFWVGKMDPRQ
                     MLNIYRYADRTIWVQPLPLTPEQKKKVIDKLERDILEENKHYAYDHFWDNCTTRVRDV
                     LDGATDGALTNLKGPEITETYRDLARKGFFGMRIPLLITDIAMGRVTDRVPTYFERMF
                     LPDYMREAAERLWGVKPIAIYERKGPPPLDDGPSGRVLFALVILLLTSPAWITRLVGR
                     FERTGLAVAILPYAFLGFVLTFLAIISPLPYVRANESCAVLIPFDILMIWFLRPQHRR
                     LYARIRVGMVVLVGLLLLVGIFKQPIWPILLWPLVPAAVVGFWPERAAATSAAPDAKK
                     PQGGKKKGSSRKN"
     gene            12297..>15035
                     /locus_tag="VFV99_31420"
     CDS             12297..>15035
                     /locus_tag="VFV99_31420"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX5063946.1"
                     /translation="MRRSRVLAACALLIGCTDAPIQRGNVVATSVDSRGVPRMLQATQ
                     LAAAPAPTAMQSARIHLERLAGEWGVAPGTLPTLAAIGEVSVAGGTITRMAQVIDGLP
                     VWGHEMRLLVRPGGELATASGTLFGTLTPRAAAHFIDDQIGAIDRAVGATYTTRTGVT
                     VEKAHAKRAWYPVADRLVAAWVVDAYTSTGSKDGDAYRTILDGATGRVLAHYSLVADA
                     SFDYSVFAETTGEKHPLDGPIVDSTPHPTGMPNGFYPAYLAGPNVVTVDSLNHTADPW
                     LDAAATETNGNNVDAYVDFNAPDGLTTGDFRADVTAPGTFGYVYDTSVGPMVSVTQQK
                     AGITSLFYILNWLHDFWYDAGFTESAGNAQLVNFGRGGEEGDPILAEGQDNALGGSRN
                     NANMATPDDGMSPRMQVYLWSGKDERSLTLPNRTPPTGGATWGPKDFDLSGTLTIGQD
                     GVGVNVTDGCTALTNSVTGKVVVVDRGNCTFKMKALNIQNAGGIAMILVNNQASTSPP
                     SMGEDSNITTPITIAALSVTDTEGAQIKTDIGNGSVTATFHRKQFQELEGSLDSTLVA
                     HEFGHYLHHRLSFCENKMCRAMSEGWGDFSALMLLARPGDNLDGAYPFSVYTTQSFSA
                     DPAYFGIRRAPYSVNPDINALSYRHMADGQALPTNHPINPSNLNSEVHNAGEIWAEVL
                     WEVYVALQKNGSDFIAVREKMAQYVVAGLLLAPNEASPMEMRNAILAAVLAANPADHD
                     AMMAAFAKRGFGSCAVAPPPESQDFTELVESTVVAGNAQLDGLVLEDTCDEDGVLDSG
                     ETATLHIKVANKGHAPLTDLQLAVTSALPGVTVMTPPTTLAKLDQFASTELEVQVKLD
                     GAADAIAGDLALSITATGGCEPTVTFPVAVRLNIDDKLEASATDTFDAATSVWDPWT"
BASE COUNT         2624 a         4892 c         4975 g         2544 t
ORIGIN      
        1 ggacttcgaa gtcgatccca ccgatgcaaa ggccgacggg ctaccaccca cgttcgatca
       61 gaacatggtc ttcaccgatg cactgctcac cgactcgctc gcgatgagcg tggatgacgt
      121 gcaggcgttc ttcgagaaca acgcgtacgg cacgcgctcg tggctcgcat cgtatcaagc
      181 cggcggcatg accgcggccg agatggtggt cgaagcggcg gtcgcgcacg agatcaatcc
      241 gatcgtgctc atcgcgcgca tgcaggtcga gacgtcactc gtcagcaaga cgaccgcgcc
      301 gtcacaacgc ttgctcgatc gcgcgctcgg ttgcggctgc ccggatggtg gcacctgcaa
      361 ccccagctac agcgggctac atcgccagct cgattgcggc gcaagaacgc tgcggcgctg
      421 gtacgacgca tcgatcgacg gcagcggaga atggctgaaa ggccgtacga ggcgcacgct
      481 cgacggccgc agcgtcacgc cgatcagtca cgcaacagcg acgctgtatg cgtacacgcc
      541 gtgggttctc gtcggcagcg gtggcacgtg gctcgcgtgg aatgtaacgc gcaaatacgt
      601 gcgtcacgcg acgacgacaa acacgatcca ttagctgaca tcgccgcacg ttaggcgatc
      661 agatcgtcct gtctgattgc ttaccgagat cacgcgagtt gggctgcgcg tgctgacgtg
      721 caacgatggc ggtcatgaga ccaccgggat ggctcgtcgc cgtgctcgtg ggcgcgttcg
      781 caggactcgt gttcgcgggc gtatccacct acgactttgt tcaacatctc gatcgacaag
      841 tgcacagcgt gcactgctcg ttcattcctg gcgcaggagc atcagcagga gcgaccggct
      901 gccaggccgc gatgatgagc tcgtactcgt cggtgttccg caccagcatc tggggcggca
      961 ttccgatctc gcttcctgcg atggccgtgt tcgcgttcat cttgttctac ggcgtcgact
     1021 tgctgctgag ccgccgcaag gatgatccgc gcgcgaccgc gttcctcgca ctcgcatgcg
     1081 cgttgccggc gctcgcgtcg gtcacgatgc tcacgatctc gctgacgaag ctcggcacgg
     1141 tgtgcaagct gtgcgtcgcg atctacatcg caagcgcgct gtgtctcacc ggcgcgatca
     1201 tcgtgtggcg tcgcgcagtg cgcggcagca ccgagtcggt cggctccgca aagaacggcg
     1261 cgtcgctcga agcacaggtc gagtcgccgc cggcgtcgaa cggctttctc ggcacgatgt
     1321 tcggcatcgg cgtcggcttc gtcgcggtgc cgctgatcct ctacatgatg atggcgcccg
     1381 atcacggcaa gttcatcggc acgtgcggcg tgctggagaa gccagacgac acgtacggcg
     1441 tgatggttcc ggtgcacccg agcggcaagg cgcaggcact cgagatcctc gatccgctgt
     1501 gccccgcatg caaagcgttc gaagcgcggc tcgaatcgtc gggcatgctc gacgatttga
     1561 atcgcaaggc gatcttgttt ccgctcgata cgacctgcaa ctggatgatc accgagaaca
     1621 cgcaccccgg tgcgtgcgcg gtgagcgagg ccgtgctgtg cgcgggcgac aaagcgcccg
     1681 aggtcatcaa atgggcgttc gaggtgcaag acagcgtgcg cgagcagacg aaggcggatc
     1741 cgaccgctgc cgagcgcatc gtgaagcagc gctggcccga gctcgcggcg tgcgtcggct
     1801 cgccggaagc aaagtcgcga ctcaacaagt cgctgcgatg ggccgtgagc aacaacatcc
     1861 gcgtgctgac gccgcagctg ttcatcgaca acgtcaagct ctgcgacgag gacgtcgaca
     1921 tcggcctcga ctacgcgctg gccaagatgc tcgacggcaa tctgcagtcg cgcgtggtgc
     1981 acaccgcgaa gccggtcgaa ggcacgtcgc cgtcgactgc gccgctgccg acgtatcagt
     2041 cgggtcagca ggccggcgag acgaagctcc cggcaacgac gccgcagccg gcgacgacgc
     2101 ctggtgagac gaagccggct gaaaccaagc ccgcagaaac gaagccgggt gaaacgaccc
     2161 cgacggaagc gaagccagcc gagacgaaac cggcggacaa gccagccgag accaagccgc
     2221 ccgaggcgaa accggcggag acgaagccgg ccgagacaaa gcccacggaa acgaaaccgg
     2281 cggagacgaa gcctgcggag ggtccggtga cgtcgccggg tggaacggac aagcccgcga
     2341 tgccggctga agagaagccg ggcaatggag gtggccaatg accccgatca gaatcggcgt
     2401 gctcgtcgtc ctgctcgcga ttcccgcggc ggcgacggca aaatggatca gctcggcgga
     2461 tagccggctg caagagatga ctgcaaatcc ggcagaagca ccggcggtgg ctgctgctgc
     2521 cgacgtcggc tactgcagtc cggagctgaa gaagatcttg cgtcgcgtgc tgatgagctg
     2581 tggtctcgtc ggcggtgaag ctgcgcgcgg ctgccagccg gtgcaggcaa agaacgttgc
     2641 gacgatgagt ggcaacgact tcaacgcgct gttccggccg atgaaggagc gtggcggcat
     2701 catccagttc gatctcgaca agagcgacct cgatcccggc gacatcgagc tcatcgacaa
     2761 ggtgttctcg gaacagaagg gtgcgagctg gttcttcgtc gtgtcgcgtg cgtcgcccga
     2821 gggctcggtc acgcacaacc gcgatctgtc gaaggcgcgc gccgagtcgg tgatgggcca
     2881 tctgcgcgag cgcttcaaag atcctgacct cgacaaggaa gtcggtctgc tctggctcgg
     2941 cgaggaattc gcgcagctcg agaccgagtt ctgcgagtgg aaacgcagca cgacaggtac
     3001 cacctgctcc cccgaggagc tcaaccggag cgcgtttgtc gcatggatcg actgtcagct
     3061 ctgatcatcg tcatcgcgct cgcaggctgt gactccggca aacaggagcc agcacccggc
     3121 cgcgccgaca cgacgaaagt cgcgaagaag gacgtcagca cggccgcgtt ctgcgacgtc
     3181 gagttcaaag gcacgagcgg cccgaagttc acggtgccca agctcgtcgg cggcgacatc
     3241 gcgccgagcg catcgacgtg gcggtggatc aacgtgtggg cgacgtggtg caggccgtgc
     3301 gtcgaagaga tgccgcgact cgcgcgctgg cgcgagaagc tcggcgcgaa gtacgagctc
     3361 tcgttcatct cggtcgacga gaacgaagca gacgtcgtcg agttcaagaa ggagcatccg
     3421 gacgcgcccg catcgccgcg gctcgccgag ccagcgaaac aggcggactg gtacgtctcg
     3481 ctcggcctcg acggtggcgc tccggtaccg atccacatct tcgtcgagcc atcaggccac
     3541 gtgaggtgtg cacgtgcggg tggtgtgcgc gagcaagact tgcccgcgat cgagaagtta
     3601 ctcgccgggc agtaatcgcg gagttagcgg ccgacggctc agaaatcgta acgcgaggtc
     3661 gtgcgttaca ctgggcatgt tgctccgcgt cctaccggtc ttgctgatgg tcaccgtcgc
     3721 tgcagccgac gccacgtcgc gcgtcgacgt cacgggcgac tacagcagca actgggacga
     3781 cgtgaagctc gtgcaggacg gcaatgaagt ccacggcacg tacgtgtgct gtggcggcgg
     3841 cacgatcgaa ggccacatct acgaaggtca cgtgattcgt taccactggc gcaacggcgg
     3901 tggtgcggac ggcgagggcg tgtgggaaat catgtcgacc gaccgcctcg agggcacctg
     3961 gggcagcggc cagagcgatg acgacggtgg ccgttgggac ctcgtccgca agtcgtcgct
     4021 cgcgaattag ctcttcggct cgacgacgcg gccgatgaac aagatcaggc cgctcgatcg
     4081 atcgacgatc aagaacagga acggatgatc ggccgtgaac ggcgtcgcct tcggtggcat
     4141 gccagcgccg cgcggcatga cgaccgcggt tgccgcggct gcctcggtgc ctttctcgtc
     4201 gaccttgacg aacgccttgt gcagcacctc gccgatgaac aggcggtcct tcgggtcggt
     4261 cggattcgag atgccggtga agtcggcgtt gtcgcgatcg aacgcgtctg tcatgccgag
     4321 cgtcttcagc gtctcggcga ggcgcatcgg ttctcccggt tcgatcgtga agcgcggcaa
     4381 cgacaccatc accgtgtcgt cgacgagctt gctctgcagc gtcctgagcg ttgccggtag
     4441 cttcttctcg agcgcaggca gtccgtcggg cttgtcgggc aacaggatgt acatcgaggc
     4501 gctgccgccc tcgtagggca gctcgagcat cgctgcgccg tcggccttcg cgagcttgaa
     4561 cgtgtcctgc ttgtgcatcg tcggcacttg tttcttctgc gtgccgtcga cgaagaaatc
     4621 cgcgggatgg gtcgccgtag gctcgaattg ctccgcccag ttcgcgagga agtagatcgc
     4681 gttgacgatc acgacgcgcg tctcgcgatc gatcgcgcgc ggcgggatca gatctttgat
     4741 gcgcttctcg gtctgctgct cgacccaggt gttgatcgcg gtgcgcacgc cgtccggatc
     4801 gttctgaaat tcggcgggct ggaccggcgc agcgaacgcg ttcttcgtga tcgccatgta
     4861 gacgggatcg aggtcgtagt gcgcgtcgac gaacagcttg ttcgcgatcc gcagcgtcaa
     4921 cgggcgcttc gggtcctgca gcgccgtcgc gatgcgtgcg aattgcgaga cgaggagatc
     4981 ggtcccaccc tcgagacgca tcgtcttctg catctgctcg cctgtcgtgc ccttcgcgcc
     5041 agcccacgcc atcgcgagcg cggtcgagat cgagagcggc gagatcgcgg tgttaccggt
     5101 gccggcgtgc gcccacagct cgagcgcgaa gttgttgctg cccttcacca gcgacgcgat
     5161 ttccttcgcg ggaggcatgt cggttttcac gtcgtgctgc acggcggtct cctgtgcatc
     5221 ggcgggcggt ggtgccgtcg gcttcgttgg cgccggcgtc ggttgcggtg atggcggccc
     5281 ctccttgctc ttgcaagcgg cgacgatggc gagggcgagg aggaagtagt gcttcatcag
     5341 tagtgcggcg gtggctcgtt ggcgggaccg agggccggct cgggcgagcg tatcgattgt
     5401 ttgagctctt tgagctcgcg ctcggtggca tcgaggcggt cgccgaacgt gcgcacgagt
     5461 gcgtcgagct cgcgaatgag cttgtcctgg aacgcgagct tgatttccag gtcttcgacc
     5521 cgttcggcca gctcttcaga catgccggca cggtagcaga cctcctgcta ctctcgtggc
     5581 gtgcttcgcc atggattgtt cgttgtcatg atcgcactcg cggcgtgctc gcacagcacg
     5641 accgagaatg acgtcgtcga attcttgaag gccaagcccg ccgaccagcg cgcgcagatc
     5701 gtgagcaaag cggatgggcg cgtcgagcgc accgacgttg tccggcgcgg tgacaaaccg
     5761 ctgacgctcg tcgggccgac gctcgatgtc ggcgatgcga tgcccgacgt cacgctcgtc
     5821 gacggcaagc tcgagccgat caagctcgcc gcgctgaagg gcaagatcgt cgtgctttcg
     5881 gtcgtgccgt cgatcgacac tcgcgtgtgc gaaacgcaga cgcacaagat cagcgacgcg
     5941 atcgcgcaga tgcctgcggg cgtcgaggtg tacacgatca gccgcgatct gccgttcgcg
     6001 cagacgcgct tcgccgagga ggcgatgagc aagacgaagt tcgcaagcga ctaccaagcg
     6061 cacgagctcg gtcgtggctt cggcctcgag gtcaaagaga gcggcctgct cgcacgcagc
     6121 gtgtgggtga tcgggacgga tgggaagatt gcgtaccgcg agctcgtcgc gaatcagggc
     6181 agcgagcccg attacgatgc gttgctcgca gccgtgaagc gcgtcgccgg cggttgaaat
     6241 gatgtggagt gcgtcgccgt cgtctgaaat cgggcgatac tggcgacctt catggcccgc
     6301 ctcgatactg atatcgcagc gatcgcgaag cgcgtcctcg aggagcaggg cttcgcgacc
     6361 gacccgccga ccgtggccgg tgcgagtccg accatcgatc ccaacctgcg cgacctgcgc
     6421 gacctgccgt ggtcgtcgat cgataacgat gaatcgcgcg acctcgatca gatcgagtac
     6481 gccgaacagc tcgatggcaa tcgcatgcgg gtgctcgtcg cgatcgccga cgtcgctgcg
     6541 ctcgtgagcc gcaactcgcc ggtcgacaag tacgccgcac aaaacaccgc gacggtgtac
     6601 acgggcgtgc gcacgtttcc gatgcttccc gaatcgctgg cgatggatcg ttcgtcgctg
     6661 ctcgaagatc gcgatcgcct cgcagtcgtc accgagatca tcgtcgacgg cgccggtgcg
     6721 ctcgacgatt cggcgaccaa ggtctatccg gcgcgcgtac ggaatcatgc caagctcgtc
     6781 tacgagcacg ttggtgcctg gctcgaaggc gacgagacag cggctccggc caacatggcg
     6841 attgccgagc aactgcggct tcacgcgcag gtgacgagct ggctgcgcga gcggcgcatc
     6901 gagtgcggtg cactcgagct cgagacgatc gaagcgcggc cgatcgcgag ggatggccgg
     6961 gttgtcgacc tcgcggtggt tcacaagaac cgcgcgcgcg atctcgtcga ggacttgatg
     7021 atcgccgtca acagcgcgac cgcgcgtttc ctcgagcgac acatgtcgtc gtcgatccgc
     7081 cgcgtcgtgc gcgcaccgaa gcgctgggat cgcatcgtcg agctcgccaa gacgtacggg
     7141 attgagttgc cggcgacgcc aaaccatcgc gcactcggag cgttcgtcgc agagcggcgc
     7201 aaggccgatg gcgagaactt cgccgagctg tcgctgtcga tcgtcaagtt gctcggtccc
     7261 ggcgagtacg tgctgcagcg tgcggctgac cccgaccagg gccacttcgg cctcgccgtc
     7321 gacgattaca tgcactcgac ggcgccgaac cgccgctatc ccgatctcgt cacgcagcgt
     7381 ttgctcaaag ctgctgctgc aagcacgccg tcgccctaca gcgacgacga cctgagtgcg
     7441 atcgcggcac actgcacgga gcgcgaagac gcagcgcgca aggtcgagcg cacgatgcga
     7501 aaggtcatcg cggcgtcgat gctgcacaag cgggtcggcg agacgttcga tgcgactgtc
     7561 accggcgcgt caccgaaggg cacctacgtc cgtctgcgcc ggccgcccgc cgagggtcgc
     7621 gtgattgttg gcgagcgtgg actcgacgtc ggcgacaaga tcaaggtgcg gctgctcgcc
     7681 accgacgtgc agcgcggctt catcgatttc gccgccacac agtgagacca gaccgagtgt
     7741 gtatcgctag tcacagcggc ctgcgttgcg gcgcgcgaga atgaggtatg cgttgggcgt
     7801 ggctggttgt cgtcgcgaca gggtgcgccg ctgacgtgcc cgcaacgccg ccacgcggcc
     7861 ttgcgctgct cgccgacaat cggctcgagg tgatgcccgg ccctgaggcg acggtgatga
     7921 tcgaacgcgc ctgcgtacgg ctcgacgatt cgttcggcgg caggtacggc ggcttgccgt
     7981 tgacgatcag gcagtacgga ggcgaaggtc aggaattcaa cttcagcgca gctgacacgc
     8041 gcttcaccgt cgcgagctcg tgcggcgaca ggcggccatc cgcagacgcc gcatcgacca
     8101 gcagctcgac gatggcgcgg cgttcatctt ccgacagctg atcgacgatc ttctccgcgt
     8161 cgtccaagcc ttccagctcg atgacgctac gcttctcggc gtcgctcaga ccgagcttcg
     8221 tcatcatgtt cgcgaggaac tcgcgctcgt cctcggccat catgccgtcg gctacgagca
     8281 ccttggagac gagcaggcac ttcgcgacgt tcgtgttcat ggctatggac tgtatgctga
     8341 gcacgcgatg ctcgtcacgt acctcgacgc gcccgatggg gtgacgctgc cggcacggat
     8401 gccaagtccg ttcggcgatg cgcatccgct tgcgcgtcat gcggcggcgg agctgcgccg
     8461 cgagctcgat gccggcctcg cgcaccggct tggcctgcac gaggacggca agatgttcgg
     8521 cgtcctcgtc gtcgaggacc gcggccgtct cgggtttttg aaagcgttct cgggcatggt
     8581 gcgtggtcag tggttactcg acggcttcgt cgggccgacg ttcgacctcg atgcgcgcga
     8641 cgcgttctgg ccggcgggcg agtgtgagct cgcggcgctc ggcgctgaca tcgccggcct
     8701 cgatcagcag cttgcaccgt tacgcgccga gctcgaactg cgcgtcgcaa cccacgagcg
     8761 cgagctcgtc gagattcgtg agcgccatcg cgcgaaccgc gcggcgcggc acgtcgaccg
     8821 cgagctacac ggcgcatcgc ccgagctcga tcaacagagt cggcgtgata ccgcggaacg
     8881 ccgcaagctc gatgcacgac atgcagccgc acgcgcagcg ctcgagcagc gcatgcagcc
     8941 gctcctcgat caacgcgcac ggatcgacgg cgagcgcacc acccgttcgc ggcacttctt
     9001 gcagcgcatc cacgcgacct acgcactcgc aaacgcgcgc ggcgacacgc gctcgctcgc
     9061 cgacgtcttc ggcgccacgc caccgcccgg tggtgccggt gattgcgctg cgccgaagct
     9121 gctcgcacac gcgtaccgcc acggcatgaa accgatcgcg ctcgccgagc tctggtgcgg
     9181 cgcatcgccg gcgactggcg gtcgcaacga cggttcgttc tatccagcgt gtcgcggcaa
     9241 gtgcggcccg attctcgcgc acatgctcgg tggcctcgat gtcgagccat cgccggtgtt
     9301 cggcgccgac gcgatcgacg cgcgcgagcc gctcacgctg ttcgaagatc gctggctcgc
     9361 gatcgtggtg aaacccgtcg gtctgctctc ggtgcccggg cgcggcacgc tgagcgactc
     9421 ggtgcaaacg cgactgcggg cacgataccc cagtgctacc ggaccgctcg tagcacatcg
     9481 gctcgacctc gatacgtcgg gggtcttact ggtcgcgaag gatcaagaca cctacgccgc
     9541 actgcagcgg tgcttcgccg agcgcacgat cgacaagcgc tacatcgcgt ggctcgatgg
     9601 caacgtccac gcggatcacg gcacgatcga gctgccgttg cgcgtcgatc tcgacgacag
     9661 gccgaggcaa atcgtcgacg agaaacacgg caaagcagca gttacggagt ggcacgtgct
     9721 cgctcgtaca accggccgga ccagagtcgc gctggtgcca aagaccggcc gcgcgcatca
     9781 gctgcgtgtg cacgcggcgc atccacgcgg cattggcgtg ccgatcgtcg gagaccgttt
     9841 gtacggccgg cctgacgctc gcctaatgtt gcacgcggag tcgatcggct tcgtccaccc
     9901 ccacacacac gagcgcctcg agcggcacag tccgtctcct ttctgaggtg cttttgctca
     9961 gaggggtttg ctagggttga ggaagtgggg ccctcgaaga cgatcggcat cgagctgtgg
    10021 ggcggccaga cccgcggcgc ttcgaacgtc gcgacgtgtt atcgcccggc gccccgcgtc
    10081 aatctcggca agccgatgcg ccagctgttc ctcggcgcgt acggcaagtt cgccgacctg
    10141 tgtcgcgagg tcgacgagcc gggcctcgca ctacttgccg tcgacgaatc gaccggtgca
    10201 ccggcaggtc tcgtgcggct acgcgctcgc gtcggccgcc acgtcgccgc gatcgtcggt
    10261 cgccacgacc aatgtgacct ctacctgagc gagcacgcgt cgctcgcgtt gcgtcacctc
    10321 gcgatcgtcc tcgatccagt gcagagctgg cggcgcaact cgacgagcgt gcgctatcgc
    10381 gtgctcgatc ttcgcacctc ggacggcttt accgacgaga acggtaagcc aatgcgcggc
    10441 ctccgcgcgg aaggcccggc cttcattcgc tgcgcggggc acgcgctgtt cgtgctgccg
    10501 ctcggcgatc cgacggactg gcccgagaat cccgctgacg cgtggtcgat gttgcccgag
    10561 cgcatctact tcgacgagat gcggtcgagc cccgacggtt cgctcacgaa catgccgctg
    10621 aatgcgtcgc gcaagagcat gatcttccgc acccacggcg tgcgtgatac cggcaccggt
    10681 gaatcgctca tcgaccgcgg cgatctcgca ggcacgctcg acgtgatcgg cgcgacgcgg
    10741 agcggccgga tgacgatcgg cgatcgcgcc ctgcgcgacg gcgtgctgct cggtcgctat
    10801 gcgcgctgcg acggcgccga gctcctcgaa gacctctcga tgtcgcgcgt gcacatgctg
    10861 ctgctgcaca ccgacgacgc cctgctcgcg gtcgacacct gcagccgcaa cggctcgcac
    10921 ctgcccggcc actccgcgac ccgcgtgatc tcagtttctg ccggcacaga gattcatctc
    10981 ggtaaacaca cgcggattcg ctggcgctgg acggcctgac gaatacactc ggggcgtgcg
    11041 ttccgcggtc tatgcgctgg tcactgcgct cgtcctcgtt gccctgacct cgtctgccaa
    11101 ggcgcagccg cgcccgagcg atccgcagga ctaccccgtc atcgagctcg tcacgatggg
    11161 cgtcggcagc ctgatctggg agcgccacgg tcacatcgcg ctgtgcgttc gctacgccga
    11221 tccgcgcgag gacgcttgct acaactacgg catcggcgac tttcaccatc cgatcgggat
    11281 ggcgactggg ttcttccgcg gcacccacag cttctgggtc ggcaagatgg atccgcggca
    11341 gatgctcaac atctatcgct acgcggatcg cacgatctgg gtgcagccgc tgccgctgac
    11401 gcccgagcag aagaagaagg tgatcgacaa gctcgagcgc gacatcctcg aggagaacaa
    11461 gcactacgcg tacgatcact tctgggacaa ctgcacgaca cgcgtgcgcg acgtgctcga
    11521 cggcgcaacc gacggcgcgc tgacgaacct gaaggggccc gagatcaccg agacgtatcg
    11581 cgacctcgcg cgcaaaggct tcttcggcat gcgcatcccg ctgctcatca ccgatatcgc
    11641 gatgggacgc gtcaccgatc gcgtgcccac gtacttcgag cggatgttcc tgcccgacta
    11701 catgcgcgag gccgccgagc ggttgtgggg cgtgaaaccg atcgcgatct acgagcgcaa
    11761 gggaccgccg ccgctcgacg atggtccgag tgggcgcgtg ctgttcgcgc tcgtgatctt
    11821 gctgctcacg tcacccgcct ggatcacccg cctcgtcggt cggttcgagc ggaccgggct
    11881 cgcggtcgcg atcctgccct atgcgttcct cgggttcgtg ctcacgttcc tcgcgatcat
    11941 cagtccgttg ccttacgtcc gagcgaatga aagctgtgcg gttctgatcc cgttcgacat
    12001 cctgatgatc tggttcttgc gcccgcagca ccgccggctc tacgcccgga tcagggtcgg
    12061 catggtcgtg ctcgttggcc tgctgctgct cgtaggtatc ttcaaacagc cgatctggcc
    12121 gattctattg tggccgctcg tgccggccgc cgtcgtcggg ttctggccag aacgcgctgc
    12181 ggcaacgtcc gccgctcccg atgcgaagaa gccgcagggc ggcaagaaga agggcagcag
    12241 ccgcaagaat tgaatcacgc cgggcgccgg aaggctcgtg ctagcgtccg agccggatga
    12301 gacgttcacg tgttctggcg gcttgtgcac tgctgatcgg ctgcacggat gcgccgatcc
    12361 agcgcggcaa cgtggtcgcg acatccgtcg attcgcgcgg cgtgccgcgc atgctgcaag
    12421 cgacgcagct ggcagccgcg cctgcgccga ctgcgatgca gtccgcgcgc attcatctcg
    12481 agcgtctcgc cggtgagtgg ggcgtggcgc cgggtacgct gcctacgctc gccgcgatcg
    12541 gtgaagtgtc ggttgccggc ggcacgatca cgcggatggc gcaggtcatc gacggcctgc
    12601 cggtgtgggg acacgagatg cgtttgctcg tgcgtcccgg tggcgagctc gcgacggcga
    12661 gcggcacgct gttcggcacg ctcacgccgc gtgcggccgc gcatttcatc gacgatcaaa
    12721 tcggcgcgat cgatcgcgca gtcggtgcga cgtacacgac gcgcacgggc gtgaccgtcg
    12781 agaaggcaca cgcaaaacgc gcgtggtatc cggtcgcgga tcgtctcgtc gcggcgtggg
    12841 tcgtcgatgc gtacacgagc acgggaagca aggacggcga tgcgtatcgc acgatcctcg
    12901 acggcgcgac gggtcgcgtg ctcgcgcatt acagcctcgt cgccgacgcc tcgttcgact
    12961 acagcgtctt cgccgagacg accggcgaga agcatccgct cgacggaccg atcgtcgact
    13021 cgacgccaca tccgaccggc atgcccaacg gcttctaccc cgcgtacctc gcgggtccga
    13081 acgtcgtcac cgtcgacagc ttgaaccaca ccgccgatcc gtggctcgac gcggctgcga
    13141 ccgagacaaa cggcaacaac gtcgacgcgt acgtcgactt caacgcgccg gatggtctca
    13201 ccaccggcga cttccgcgcg gacgtcacgg cgccaggcac gttcggttac gtctacgaca
    13261 cgtcagtcgg gcccatggtc tcggtgacgc agcagaaggc cggcatcacg tcgctgttct
    13321 acatcctcaa ctggctacac gacttctggt acgacgccgg cttcaccgag agtgcaggca
    13381 acgctcagct tgtcaatttc ggtcgcggcg gcgaagaggg cgatccgatc ctcgccgagg
    13441 gccaggacaa cgcgctcggt ggctcgcgca acaacgcgaa catggcgacg cccgacgacg
    13501 gcatgtcgcc gcgcatgcag gtctatctgt ggagcggcaa ggacgagcgc tcgctcacgc
    13561 tgccgaatcg cacgcccccg acgggcggcg cgacgtgggg accgaaggac ttcgatctct
    13621 ccggcacgct cacgatcggc caggacggcg tgggcgtgaa cgtgaccgat ggctgcaccg
    13681 cgctgacaaa ctccgtgacc ggcaaggtcg tcgtcgtcga tcgaggcaac tgcacgttca
    13741 agatgaaggc gctcaatatc cagaacgccg gcggcatcgc gatgatcctg gtcaataacc
    13801 aggcgagcac gtcgccgccc tcgatgggcg aggattcgaa catcaccacg ccgatcacga
    13861 tcgctgcgct gtcggtcacc gacaccgaag gcgcgcagat caagaccgat atcggtaacg
    13921 gttcggtcac cgcgacgttc caccgcaagc agttccagga gctcgaaggc tcgctcgact
    13981 cgacgctcgt cgcgcacgag ttcggtcact acctgcatca ccgcctgagc ttctgcgaga
    14041 acaagatgtg ccgcgcgatg agcgaaggct ggggtgattt cagcgcactg atgctgctcg
    14101 cgcgccctgg cgacaacctc gacggcgcgt acccgttcag cgtctacacg acgcagagct
    14161 tctcggcaga cccggcgtac ttcggcatcc gccgcgcgcc gtacagcgtg aacccggaca
    14221 tcaacgcgct ttcgtaccgc cacatggcgg atggccaggc actgccgacg aatcatccga
    14281 tcaatccgtc gaacctcaac tccgaggtgc acaacgccgg cgagatctgg gcggaggtgc
    14341 tgtgggaggt ctacgtcgcg ctgcagaaga acggctctga cttcatcgcc gtgcgcgaga
    14401 agatggcgca atacgtcgtc gcggggctct tgctcgcgcc gaacgaagcg tcgccgatgg
    14461 agatgcgcaa cgcgatcctc gctgctgtgc tcgcagcgaa tcccgcggac cacgacgcga
    14521 tgatggccgc gttcgcgaag cgtggattcg gcagctgtgc ggtggcgccg ccgccggaga
    14581 gccaggactt caccgagctc gtggagagca cggtcgtcgc tggcaacgca cagctcgatg
    14641 gcctcgtgct cgaagacacc tgcgacgagg acggcgtgct cgacagtggc gagaccgcaa
    14701 cgcttcacat caaggtcgcc aacaagggtc acgcgccgct caccgacctc cagctcgcgg
    14761 tgacctcggc gctgcccggc gtgacggtca tgacgccgcc gaccacgctg gccaagctcg
    14821 atcaattcgc gtcgacggag ctcgaagtgc aagtgaagct cgacggcgcc gccgacgcta
    14881 tcgccggcga tctcgcactt tcgatcaccg ccaccggcgg gtgcgagccg accgtgacgt
    14941 tcccggtcgc agtccggctc aacatcgatg acaagctgga agcgtcggcg accgacacgt
    15001 tcgatgctgc gacgagcgtg tgggatccgt ggacg
//
LOCUS       DASUFX010000777         4510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1655372, whole genome shotgun sequence.
ACCESSION   DASUFX010000777 DASUFX010000000
VERSION     DASUFX010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655372"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFV99_31425"
     CDS             complement(<1..505)
                     /locus_tag="VFV99_31425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063947.1"
                     /translation="MVDPLLDTVVNGRYQVVRLLGEGGMSVVYEVQHVKLKRQFALKR
                     LLPALINHEEALYRFEREAEVLASLHHPNIVEVTDWDALPDGSPFMILEFLHGAHIRV
                     RIDRGPLPWEAIARIGDQTMSALALAHRLGVTHRDLKPENIFISIDDAGEERVKLLDF
                     GVSKLRGV"
     gene            complement(561..1370)
                     /locus_tag="VFV99_31430"
     CDS             complement(561..1370)
                     /locus_tag="VFV99_31430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063948.1"
                     /translation="MTRSLLLSAFIGLLATGCVVLPTTKTTVKNAGTEKSPLTYGRVK
                     AVSLQTGSSRTDVRVRVLSTRECSRQILAVKEITKSKHARLGVDDPRGRAVGIVLSPV
                     TMPISAIITGIIVASSDDETTRVTKPLRIETTECHTDAAEYPLELEFPTGHIYRGKTD
                     KNGVLALAIPRDEPYVGQVAVRSTQATAELHYEQTLPPVTATRDAIESCRTENNVDGV
                     TAKLTIDTRGFATRLWLSAGDAKLHACVATKIAGVVFPAALHNATVVLPST"
     gene            1512..2480
                     /locus_tag="VFV99_31435"
     CDS             1512..2480
                     /locus_tag="VFV99_31435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063949.1"
                     /translation="MKTCAALVVLASLTRLAHADERVVTLDRTCVAIDDADGLAATDR
                     EYAVALMQRVLERADLFVVASDCTETYTISHQQVDGRFVIRVRNSAGKRRMTTDAMNE
                     LSEKYERMVRSLIQAKEAQELSQAAMPEPATATKVVATAQEAAATPDSAPLPTLQATD
                     ETSTSETTDGTEYDGDVKQRMWYGLAGLQLTGGGGVTMGYRHELATTTIDIAGNLRGA
                     DTGTAGVALGVELLKYHWSSPTRAVYGGGGLSVGSLDRSDQYGANHYWGSGLQGELTA
                     GAQFGGKTGVRGLIQLDITLPFFQMGNDNGDKEYVAAAMVSGGLGW"
     gene            2509..3393
                     /locus_tag="VFV99_31440"
     CDS             2509..3393
                     /locus_tag="VFV99_31440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063950.1"
                     /translation="MLRLSLVAVAVFASASPAFAQNAFDVCKGESKTAVLGQGKISIA
                     VVYPEDFPITTTPAQRNAVGAALAKAEKGIIVPAKDVEAAKKLVDAKKWTEKSAACGY
                     SPSLVPVLGLKHKNLSTAHASVVCEGDKCVLRVDLERHGKSSSERWVRYEAPLSGPKD
                     KVATIQAAAPKLANKGVPADASTKGLAVKELVDGTVTVRSDIDGALELDRIMEAAPAI
                     AACTPKKKKAKDIRGYWADWKLSAQGTAMEVMVKPFGGVDPTDDAAAKCLAQALRNLQ
                     MSCPRDGKPAPVRTAICL"
     gene            3419..4087
                     /locus_tag="VFV99_31445"
     CDS             3419..4087
                     /locus_tag="VFV99_31445"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5063951.1"
                     /translation="MVRLVVLALLYTSTAAFADDPPAIRYAAMTQEACEAELATRHIE
                     FDREAAKGVLAPVRLKGSLHGVEFRTNLKDDQRATTPWEIADCRLVLAMDDFAQILEQ
                     HGVVQVRHYSMYRTAPKRWPDDKIGGQHNGGLALDAARFIRKDGTKLDVNDDFKGRVG
                     AKTCGPKARKAKTPEALELREIVCAAVDQQLFNVVLTPDFNRRHRNHFHLEVTAGVKW
                     FMVR"
     gene            4087..>4510
                     /locus_tag="VFV99_31450"
     CDS             4087..>4510
                     /locus_tag="VFV99_31450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063952.1"
                     /translation="MARHAAIVVLLAACSRPPAPKDPTERALYRDLERQVTVKAATGW
                     GIDKLTIDSMLDSALDSVCRVDVLGRRGLRAWLDNEIARLGGPVEDAWRKRGKKLSKV
                     DDLLVLTRVHLLLDRADELSLDCPFWLEPQNPFHGRQIS"
BASE COUNT          804 a         1472 c         1507 g          727 t
ORIGIN      
        1 caacgccgcg cagcttcgac acgccgaagt cgagcagctt cacacgctct tcgccggcgt
       61 cgtcgatcga gatgaagatg ttctcgggct tgaggtcgcg gtgcgtgacg ccgagccggt
      121 gcgcgagtgc gagcgccgac atcgtctgat cgccgatgcg cgcgatcgct tcccacggca
      181 gcggcccacg gtcgatgcgc acgcggatgt gcgcgccgtg gaggaattcc aggatcatga
      241 acggcgagcc gtcggggagc gcgtcccagt ccgtgacctc gacgatgttt gggtgatgca
      301 gcgacgcgag cacctcggct tcgcgctcga agcgatacag cgcttcctcg tggttgatca
      361 gcgccggcag cagccgcttg agcgcgaact gccgcttcag cttcacgtgc tgcacctcgt
      421 agacgacact catgccgcct tcaccgagca ggcgcacgac ctggtaccgg ccgttcacca
      481 cggtgtccaa cagcggatct accacttgcc cgatagtaca tgctgtcggc cgcccggtct
      541 ggcccccatg tagggcgcgg tcaggtcgat ggcagcacga cagttgcgtt atgcagcgct
      601 gccggaaaaa ccacgcccgc aattttcgta gctacacacg cgtgcagctt cgcgtcccct
      661 gccgacagcc agagccgcgt cgcgaagccg cgagtgtcga tcgtcagctt ggccgtgacg
      721 ccgtcgacat tgttctcggt gcggcagctc tcgatcgcat cgcgcgtcgc ggtcaccggc
      781 ggcagcgtct gctcgtagtg cagctctgca gtcgcttgcg tgctgcgtac cgcgacctgg
      841 ccaacgtacg gctcgtcgcg cggaatcgcc aacgcgagca cgccgttctt gtcggtcttg
      901 ccgcgataga tgtggccggt cggaaactcg agctcgagcg gatactcggc cgcgtcggtg
      961 tggcattccg tggtctcgat gcgcagcggt ttcgtcacgc gcgtcgtctc gtcgtccgag
     1021 ctcgccacga tgatgccggt gatgatcgcc gagatcggca tcgtcaccgg tgagaggacg
     1081 attccgaccg cccggccgcg cggatcgtcg acaccgagcc gcgcgtgctt gctcttcgtg
     1141 atctctttga ccgcgaggat ctgacgcgag cactcgcgcg tgctgagcac gcggacgcga
     1201 acatccgtgc gcgagctacc ggtctggagg ctcaccgctt tgacgcgccc gtaggtcagc
     1261 ggcgacttct cggtgcccgc attcttgaca gtggtcttgg tggtcggcag cacgacgcag
     1321 ccggtggcga ggaggccgat gaaggcagag aggaggagcg agcgcgtcat gtcgtaaggc
     1381 tcgccgaacg acccgtgagc tcgcctggaa tgcgccgtga gcttcgcgtg aaccgccgag
     1441 agtcgtggcc tcacgcgcac ttcacgcggc gccgctgtcg gcttcacgtc cgggaatggc
     1501 gtgatgtcga catgaagacc tgcgctgctc tggttgtcct ggcctcgctg actcgcctcg
     1561 cgcatgccga cgagcgcgtc gtcacgctcg accgcacgtg cgtcgcgatc gatgacgcgg
     1621 atggcctcgc cgcgacggac cgcgaatatg cagtggcgct gatgcagcgc gtgctcgagc
     1681 gcgcggactt gtttgtcgtc gcgagcgact gcaccgagac ctatacgatc tcgcatcagc
     1741 aggtcgacgg ccgcttcgtg atccgcgtgc gcaactcggc gggcaagcgc cgaatgacga
     1801 cagacgcgat gaacgagctc tcggagaagt acgagaggat ggtgcgctcg ctcatccaag
     1861 cgaaggaagc gcaagagctg tcccaagcgg cgatgcccga accggccaca gcgaccaagg
     1921 tcgtcgcgac ggcgcaagaa gcggcggcga cgccagacag tgcaccgctg ccgacgctgc
     1981 aggcgacgga cgagacgtcg acgtccgaga ccacggacgg caccgaatac gacggcgacg
     2041 tcaagcaacg catgtggtac ggcctggccg gcctgcaact caccggcggc ggcggggtga
     2101 ccatgggcta tcgccacgag ctcgcgacga ccaccatcga cattgccggc aacctgcgcg
     2161 gcgcggatac cggaacggct ggcgtagcgc tcggcgtgga gctgttgaag taccactgga
     2221 gctcaccaac gcgagcagtg tacggcggcg gcggcctctc ggttggcagc ctcgaccgta
     2281 gcgatcagta tggcgcgaat cactactggg gcagcggtct gcaaggcgag ctcaccgccg
     2341 gtgcgcagtt cggcggcaag acgggcgtgc gcgggctcat ccagctcgac atcacgttgc
     2401 cgttctttca gatgggtaac gacaacggcg acaaggagta cgtcgcagca gccatggtct
     2461 ccggcggtct tggctggtga tcacctcccg ttcacgtatc atctagccat gcttcgtcta
     2521 tctctcgtcg cggtcgccgt cttcgcctcc gcctcgcccg ccttcgcgca gaacgcgttc
     2581 gacgtgtgca aaggcgagtc gaaaacagcc gtgctcggcc agggcaagat ctcgatcgcc
     2641 gtcgtctatc ccgaggactt cccgatcacg acgacgccgg cacagcgcaa cgcggtcggt
     2701 gccgcactcg ccaaagcgga gaagggcatc atcgtccctg cgaaggatgt cgaggcggcg
     2761 aagaagctgg tcgacgcgaa gaagtggacc gagaagagcg cggcgtgcgg gtactcgccg
     2821 tcgctcgtgc cggtgctcgg cctcaagcac aagaacctgt cgacggcgca cgcgagcgtc
     2881 gtctgtgagg gtgacaagtg cgtgctgcgc gtcgacctcg agcgacacgg caagagctcg
     2941 tccgaacgct gggtacgcta cgaagcgcca ctgagcgggc cgaaggacaa ggtcgcgacg
     3001 atccaagccg cggcaccgaa gctcgcgaac aaaggagtac cggccgatgc gtcgacgaag
     3061 ggcctcgcgg tcaaggagct tgtcgacggc acggtgaccg tacgcagcga catcgacggc
     3121 gcactcgagc tcgatcgcat catggaagcg gcgccggcaa ttgccgcgtg cacgccgaag
     3181 aagaagaagg cgaaagacat ccgcggctac tgggcggact ggaagctgtc ggcgcaaggc
     3241 acggcgatgg aagtgatggt caagccgttc ggcggcgtcg atcccaccga cgatgccgct
     3301 gcaaagtgcc tcgcgcaggc gcttcgcaat ctgcagatgt cctgcccgcg agacggcaag
     3361 cccgcacccg tgcgcaccgc gatctgcctg taacccgcga gctgttatat ggtgcggcat
     3421 ggtccggctc gtggtgctcg ctctgctcta cacgagcacg gccgcgtttg ccgatgaccc
     3481 gccagcgatt cgctacgcgg cgatgacgca ggaggcttgc gaggccgagc tcgcgacgcg
     3541 ccacatcgag ttcgatcgcg aggccgcgaa gggcgtgctc gcaccggtgc ggctcaaggg
     3601 ctcgctgcac ggcgtcgagt tccgcacgaa cctcaaggac gatcagcgcg cgacaacgcc
     3661 gtgggaaatt gccgactgcc ggctcgtgct cgcgatggat gacttcgccc agatcctcga
     3721 acagcacggc gtcgtacagg tgcggcacta ttcgatgtat cgcaccgcac cgaagcggtg
     3781 gcccgacgac aagatcggcg gccagcacaa cggcgggctc gcgctcgacg cggcgcgctt
     3841 catccgcaag gacggcacca agctcgacgt gaacgacgac ttcaagggtc gcgtcggcgc
     3901 caagacgtgc gggccgaaag cgcgcaaggc gaagacaccg gaggcgctcg agctccgcga
     3961 gatcgtgtgc gcggccgtcg atcagcagct gttcaacgtc gtgctcacgc ccgatttcaa
     4021 tcgccgtcat cgcaatcact tccacctcga agtcaccgcc ggcgtgaagt ggttcatggt
     4081 gcggtgatgg ccaggcacgc agcgatcgtt gttttgctcg ccgcgtgcag ccgaccgcca
     4141 gcaccgaagg atccgaccga gcgcgcgctg taccgcgatc tcgagcggca agtgaccgtg
     4201 aaggcagcca cgggctgggg catcgacaag ctcacgatcg attcgatgct cgacagcgcg
     4261 ctcgactcgg tgtgccgcgt cgacgtgctc ggccgccgcg gtctgcgcgc gtggctcgac
     4321 aacgagatcg cgcggctcgg cggtcccgtc gaggacgcat ggcgcaagcg cggcaagaaa
     4381 ctatcgaagg tcgacgacct gctcgtgctc acgcgcgtac acctgttgct cgatcgcgcc
     4441 gacgagctgt cgctcgactg tccgttctgg ctcgagcccc agaatccatt ccacggccgg
     4501 cagatctccg
//
LOCUS       DASUFX010000778         5836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_960190,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000778 DASUFX010000000
VERSION     DASUFX010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960190"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /gene="mtnA"
                     /locus_tag="VFV99_31455"
     CDS             complement(<1..759)
                     /gene="mtnA"
                     /locus_tag="VFV99_31455"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5063953.1"
                     /translation="MKVEGRWRRTIELADDKASVNVLDQAALPYEIRWLNLGTLEDVA
                     RAIREMHIRGAPLIGATAAYGVAIALRHDPKSIDEACTVLAATRPTAINLRWALDDMR
                     RRVADVPVSERAAVAFAAAAEICDEDVAMCEAIGQHGFTLIKVAAERAGNRPVRVLTH
                     CNAGWLATVDWGTALAPVYKAFEAGIPLHVWVDETRPRNQGMLTAWELAQHGVAHTVI
                     ADNAGGHLMQRGKVDLCIVGSDRTTARGDVCNKVG"
     gene            complement(749..1279)
                     /locus_tag="VFV99_31460"
     CDS             complement(749..1279)
                     /locus_tag="VFV99_31460"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5063954.1"
                     /translation="MNFKSLIRTVPNFPKQGIMFRDITPLLASGTGFRHVVSVLADRY
                     AGKVDKVVAIEARGFIFGAAIAHQLGAGFVPIRKPGKLPHTSIGHDYELEYGTNRVEV
                     HVDGLAKGERVVVIDDLLATGGTALAALSLVEKSGASVVECAFVIELLDLPGRTRIVE
                     RGHRVHALCGFTEDES"
     gene            complement(1276..2190)
                     /locus_tag="VFV99_31465"
     CDS             complement(1276..2190)
                     /locus_tag="VFV99_31465"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF006492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5063955.1"
                     /translation="MIGIVGGTGLYNLDGLTEQRWLRVDSPFGTPSDELLFGKLGDQR
                     VVFLPRHGRGHRIPPHEINFLANIDALKRAGVTSIVSVSAVGSLREDLPPGTFVLVDQ
                     MIDRTLRKPKTFFGTGLVAHVSMGHPTCRRLIDHLYVSGASLGLSIVKGGTYMVMEGP
                     QFSSMAESEFHRGLKCDVIGMTNMPEAKLAREAELCYATVAMVTDFDCWHPDHDHVQV
                     ADIVKVLHANSDNARKLLKEVIPRVGADAGPCQHGCDRALEYAVMTAPEARDPELVKK
                     LDAVAGRVVVPGGRSESARLHFWPGGVA"
     gene            2316..3062
                     /locus_tag="VFV99_31470"
     CDS             2316..3062
                     /locus_tag="VFV99_31470"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5063956.1"
                     /translation="MVFETLTARLLAAQDFIEVAMAVCVLGRQQMGVHQTVVTLQALD
                     GKPLLMVDDVPNVSDEQRLAWVTELWPRDPYQPPMREHHAPVGDEHLSTPETDKVSRD
                     MGYRGDYVHMLLLPILQPGQLLGTIRCGHLHGFTPDLRRDLTTLSSHVSVRLAQLGIT
                     TVPCPLLAKLTPRQRDVAQLAARGYTNADIGTRLELSENTVKKHLKDIFDLLEIANRT
                     ELAARLSKSPQHNVPVGVSRRGDVSITRAP"
     gene            complement(3367..4116)
                     /locus_tag="VFV99_31475"
     CDS             complement(3367..4116)
                     /locus_tag="VFV99_31475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063957.1"
                     /translation="MATRAAKKATVKRAAKRPAKRVSAKPRRGIAKRRQPIRDAKPPV
                     VEQSQIDGIIANADYALREHIHGDLVANAITLLRGRSHVAGDLALDFDGDDDCPDGTI
                     VDGDLEVSGSIINRDSDAGPFLLVTGNVHAKNLVAGGSEIVILGDLIVDEVIFGYYNH
                     GSITVHGTTRAKAIITEYHSFDLRGRVEGITVSGRGRITADDHFRSYAPALVADVLTD
                     GNPSGGYPDWDLTTEAILSGRPVLRDDVVLN"
     gene            complement(4135..>5836)
                     /locus_tag="VFV99_31480"
     CDS             complement(4135..>5836)
                     /locus_tag="VFV99_31480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5063958.1"
                     /translation="NIDPVVVFTADVARYRATGDHDALISELGIAAREPTLGRAAIEE
                     LALELHRALVRADTDRALEAARQLVRALRRGRGDAASRTGGIAALARVVEWARDRRSA
                     ELSLLRTDLLELVRELVEQGASKPAPDTALVSLMHKPPGPSTSDDAAEVAAALRLYGD
                     LLASADAVDPEDLRRIARALGEPDDDVRTAAEEALSALGPAAAGELIATAAWGRRRAR
                     DRAAALLADLPVTPVTIDRLVDAELDALEQTAAAISILAEPGDELLARRLDERLREIA
                     HTVLLLVAARRRSRAIARAAIAWRHARAGQERARTLAVIEAALPRALVGRLVEAVDDL
                     TPADRAAALARAGIAPPQRDAVMRAELAGRDLLSRALVLHSLGSAGRSAHRDAIAKAA
                     QAEAAAASATDLLRRLTEAVDDPVDEGASDMPTRVETLIALGRVPLLAALSTRQLADV
                     AERARWVTAREGSVLITAGDPIDALIVVEDGELHLGSRRIVKGEVVDELACVAPTAAS
                     ADLGVARNARLIRLERVDFEELVDDVPGLASSVCRALGERARRAEDGVYRSPLASRGG
                     "
BASE COUNT          973 a         1970 c         1947 g          946 t
ORIGIN      
        1 gcccaccttg ttgcagacgt cgccgcgggc cgtggtgcgg tccgatccca cgatgcagag
       61 atccaccttc ccgcgctgca tcaggtggcc gccggcgttg tcggcgatca cggtgtgcgc
      121 gacgccgtgc tgcgcgagct cccacgcggt caacatgccc tgattgcgcg ggcgcgtttc
      181 gtcgacccag acatgcagcg ggatgccggc ctcgaatgcc ttgtagaccg gcgcgagcgc
      241 ggtgccccag tcgacggtcg caagccaccc cgcgttgcag tgcgtgagga ctctcacggg
      301 gcgattgccg gcgcgctcgg ccgccacctt gatgagcgtg aagccatgct ggccgatggc
      361 ctcgcacatc gccacgtcct cgtcgcagat ctcggcagca gcggcgaacg caacggccgc
      421 gcgctcgctc accggcacgt cggcgactcg gcgtcgcatg tcgtcgagcg cccagcggag
      481 gttgatcgcg gtcggccgcg tcgcggcgag caccgtgcat gcctcgtcga tcgacttcgg
      541 atcatgccgc agcgcgatcg cgacgccgta tgcggcggtg gcaccgatca gtggcgcgcc
      601 gcggatgtgc atctcgcgaa tcgcacgcgc gacatcctcg agcgtgccga ggttcagcca
      661 gcggatctcg tatggcagcg ccgcttgatc gagcacgttg accgacgctt tgtcgtcggc
      721 gagctcgatg gtccggcgcc agcggccttc aactttcatc ttcggtaaac ccgcagagag
      781 cgtggacgcg atggccgcgt tcgacgatcc gggtacggcc gggtagatcg agcagctcga
      841 tcacgaacgc gcattcgacg acgctcgccc ccgacttctc gacgagcgag agagccgcga
      901 gtgcggtgcc gccggttgcg agcaaatcgt cgatgacgac gacgcgctcg cccttcgcga
      961 gaccatcgac gtgaacctcg acgcgattcg tgccgtactc gagctcgtag tcgtggccga
     1021 tcgacgtgtg cggcagcttg cccggcttgc gaatcggcac gaagccggcg ccgagctggt
     1081 gcgcgatggc ggcgccgaag atgaagccgc gcgcttcgat cgcgacgact ttatcgacct
     1141 tgcccgcgta gcggtcggcg agcacgctca cgacatggcg aaagccagtg ccgctcgcga
     1201 gcagcggcgt gatgtcgcga aacatgattc cctgtttcgg aaagttcggg accgtgcgaa
     1261 tgagcgactt gaagttcacg caacacctcc tggccagaag tggaggcggg cggattcact
     1321 ccgcccgccg gggacgacga cgcgacctgc gacggcgtcg agctttttca cgagctcggg
     1381 atcgcgggct tccggtgcgg tcatgacggc atattcgagc gcgcgatcgc agccgtgctg
     1441 gcacggtcct gcatcggcac ctacgcgcgg aatcacttcc ttgagcagct tgcgcgcgtt
     1501 gtcgctgttc gcgtggagca ccttgacgat gtcggcaact tgcacgtgat cgtgatcggg
     1561 gtgccagcag tcgaagtcgg taaccatcgc gacggtcgcg taacagagct cggcttcgcg
     1621 cgcgagcttg gcctccggca tgttcgtcat gccgatcacg tcgcatttga ggccgcggtg
     1681 gaattcactt tcggccatgc tcgagaactg cgggccttcc atcaccatgt acgtcccacc
     1741 tttgacgatc gacagcccga gcgacgcccc ggacacgtat agatgatcga tcaggcggcg
     1801 gcacgtcgga tgccccatcg acacgtgcgc gacgaggccg gtgccgaaga acgtcttcgg
     1861 cttgcgcagt gtccgatcga tcatctgatc gacgagcacg aacgtgcccg gcggcagatc
     1921 ttcgcgtaac gaccccaccg cactgaccga aacgatcgat gttacaccgg cacgcttgag
     1981 cgcgtcgatg ttcgcgagga agttgatttc gtggggcggg atacgatggc cgcggccgtg
     2041 tcgcggcaag aacacaacgc gctgatcgcc aagcttgccg aacaaaagct cgtccgatgg
     2101 tgtgccaaat ggcgaatcga cacgtagcca gcgctgttcc gtgaggccgt cgaggttgta
     2161 gagccccgta ccacccacga tcccgatcac cgtcgaggag gacatcactg gggacgtacc
     2221 atcttgcgcg cgcgtgaaca acttgcgggc gcgtgaacaa cacgtgaagc acccccgcga
     2281 ccggtaaacc ttgtgcgaga gtggcttcac tcgtcatggt ttttgagacg ctgaccgccc
     2341 gcctacttgc cgcgcaggac ttcattgaag ttgcgatggc cgtgtgcgtg ctcggccgcc
     2401 agcagatggg cgtgcatcag acggtcgtga cgctgcaggc actcgacggc aaaccactgc
     2461 tcatggtcga cgacgtaccg aatgtgagcg acgagcaacg cttggcctgg gtcaccgagc
     2521 tgtggcctcg ggatccgtac cagccgccga tgcgcgaaca tcacgcgccc gtgggtgacg
     2581 aacacctgag cacgcccgag accgacaaag tgagtcgcga tatgggctat cgcggcgact
     2641 acgtacacat gctgctgctg ccgatcctgc agccgggcca gttgctcggc acgatccgct
     2701 gcggtcacct ccacggcttc acgcccgatc tgcgccgcga tctcaccacg ctgagcagcc
     2761 acgtctcggt ccgcctcgcg cagctcggta tcaccacggt tccgtgcccg ctgctcgcga
     2821 agctcacgcc gcgccagcgc gacgtcgctc agctcgccgc gcgcggctac acgaacgccg
     2881 acatcggcac gcgcctcgag ctgtcggaga acacggtcaa gaaacacctc aaggacatct
     2941 tcgacctgct cgagatcgca aaccgcacgg agcttgcggc gcggctctcg aagagcccgc
     3001 agcacaacgt tccggtcggc gtcagcagac gcggcgacgt ctcgatcacg cgcgcgccgt
     3061 aggccagccg agaacccgag cgcacgacgt gcgattgaag ccgcagtggc tcgtcgtcgt
     3121 tcgagtgccg atggcgcctt cggcgcggca cgacgtgttc cagatctaac gccaagaacg
     3181 ccaagcgaag cccgccaaga acgccaccgg aagcttgatc ttcgacgcgg tctgcagccg
     3241 tgatcgtgcg cgcagaccgc gatcccgaaa cctttcttct ggcgttcttg gcgtcttggc
     3301 gccttggcgt tcctccgggc gccgcgaagc ggcagacgcg ccttccaggc gatgcgctac
     3361 acgctgctag ttcaagacga cgtcgtcgcg cagcactgga cggccggaga ggatcgcctc
     3421 ggtcgtcaga tcccagtccg ggtagccgcc cgacgggttg ccatcggtga gcacgtcggc
     3481 gacgagcgcc ggcgcatacg aacggaagtg atcgtctgcg gtgatgcggc cgcggccact
     3541 caccgtgatg ccttcgacgc gaccgcgcag atcgaacgag tgatactcgg tgatgatcgc
     3601 cttcgcgcgc gtcgtaccgt gcaccgtgat cgagccgtgg ttgtagtacc cgaagatcac
     3661 ctcgtcgacg atcagatcgc cgaggatcac gatctcggaa ccgcctgcga ccaggttctt
     3721 cgcgtgcacg ttgccggtga cgagcaagaa cgggccagcg tcgctgtcgc gattgatgat
     3781 cgagccactg acttcgagat cgccatcgac gatcgtcccg tccgggcagt catcgtcgcc
     3841 atcgaagtcg agcgcgagat caccagcgac atgggagcgc ccccggagca acgtgatcgc
     3901 gttcgccacg aggtcgccgt gaatgtgctc gcgcagcgcg tagtcggcat tggcgatgat
     3961 gccgtcgatc tgcgattgct cgacgacggg tggcttggcg tcgcgaatcg gctgccgacg
     4021 cttcgcaatc ccgcgccggg gcttcgcaga gacgcgcttg gccgggcgct ttgccgcccg
     4081 cttcaccgtc gccttcttcg ccgcccgtgt ggccatctat atcgacggta acactcagcc
     4141 gccacgactg gcgagcggcg atcggtacac accgtcctcc gcgcggcgtg cacgctcgcc
     4201 aagcgcgcga cataccgatg acgcaaggcc gggcacatcg tcgacgagct cttcgaaatc
     4261 gacacgttcc aggcggatca gccgggcgtt gcgcgcgaca ccgagatcgg ccgacgcggc
     4321 cgttggcgcg acacatgcga gctcgtcgac aacttcgccc ttgacgatgc ggcgactgcc
     4381 gaggtggagc tcgccgtcct ccaccacgat cagcgcatcg atcggatcgc ccgcggtgat
     4441 gagcaccgag ccttcgcgtg cagtgaccca ccgtgcccgc tcggcgacat cggcgagctg
     4501 ccgcgtcgac agcgcggcga gcaatggcac acggcccagg gcgatgagcg tttcgactcg
     4561 agtcggcatg tcactggctc cttcgtcgac gggatcgtcg accgcctcgg tgaggcgacg
     4621 taacagatcg gtggcgctcg cggcagcggc ttcggcttgc gcggctttcg cgatcgcatc
     4681 gcgatgcgcg ctgcgcccgg cggacccgag cgagtgcaga acgagcgcgc gggacagcag
     4741 atcgcggccg gcgagctcgg cacgcatcac ggcatcgcgc tgcggcgggg caatgccagc
     4801 gcgagccagt gcggcagccc gatcggcggg cgtcaggtca tcgacagctt cgacgaggcg
     4861 gccgaccagc gcccgtggca gtgcagcttc gatcaccgcg agcgtgcgcg cgcgttcctg
     4921 gccggcacgg gcatggcgcc acgcgatcgc ggctcgcgcg atcgcacgcg accgccggcg
     4981 cgcggcaacg agcaacagca cggtatgcgc gatctcgcgc aggcgctcgt cgagccggcg
     5041 cgcgagtagc tcgtcgcctg gctcggcgag aatcgagatc gcggcggctg tctgctcgag
     5101 cgcgtcgagc tcggcgtcga cgagccgatc gatcgtgacc ggcgtgaccg gcaggtcggc
     5161 gagcaacgcg gcagcgcgat cgcgtgcgcg gcgacgtccc catgccgccg tcgcgatcag
     5221 ctcaccggcc gctgctggcc cgagcgcgga caacgcttcc tcggcggcgg tgcgcacgtc
     5281 gtcgtcgggt tcgccgagcg cgcgcgcgat gcggcgcagg tcctcgggat cgacggcatc
     5341 ggcgctcgcg aggaggtcac cgtacagccg cagtgccgcg gcgacttccg cggcgtcgtc
     5401 actcgtcgat ggtcccggtg gcttatgcat cagcgagacg agcgcggtgt ctggcgccgg
     5461 cttgctcgca ccctgctcga ccagctcgcg cacgagctcg agtagatcgg tgcgcaacag
     5521 cgatagctcg gcgctgcgcc gatcgcgcgc ccactcgacg acgcgggcga gcgctgcgat
     5581 accgccggtc cgactcgctg catcgccacg gccacgccgc agtgcacgca cgagctggcg
     5641 cgctgcttcg agcgcgcggt cggtatcggc gcgaacgagc gcgcgatgga gctcgagtgc
     5701 cagctcctcg atcgcggcac gtcccagcgt gggctcgcgc gccgcgatgc cgagctcgct
     5761 gatcagcgcg tcgtgatcgc cagttgcgcg atagcgtgcg acgtccgcgg tgaagacgac
     5821 aacggggtcg atattc
//
LOCUS       DASUFX010000779        12855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_820693,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000779 DASUFX010000000
VERSION     DASUFX010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820693"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VFV99_31485"
     CDS             <1..2020
                     /locus_tag="VFV99_31485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063959.1"
                     /translation="NQDDETDDCPDGPSCPQCSNNKDDDGNGQTDYPNDSGGCASASD
                     TDEYTRNPVACGSNVMIKILPFDGHAMGTIGNMGASSLVSPTCGGAGLEDVYELRVTS
                     PKVIVASTDSDATGIDTVLYIRGSDCANMSSEMVCNDDISTTDKKSTVTKSITTPGTY
                     YLVVDAHDTSVSGSYDLTVKFLTGEGETCSGADDCGPGLVCRIPGGGTQKICTKHVCE
                     DNIDDDMDGKLGYPTDPGCSSATDDDETDGCPGVGPNCPECGDGVDNDGDGKTDYGTN
                     GDMTCTSAADASEACVSQEGVQAITTAMTMGDTTGQHNDVVPSCGSTTSTAPDRTYRL
                     DVPAMANLQLNLTTSFDTVTALYNSTCGGTAVACSDPTTMSVNNLAAGTYYFVVDGYF
                     STSVGTYTINVLGKIQNGQSCESALAQSGAITCNTGYACKGTMGSRTCQPALCSDGLD
                     NDTDGKIDYPNDPGCVDSADDTEQNPTTLPVCADGSDNDTDGLTDFPADYGCAAASGT
                     SEVFCMGEMDTTAAIATKTTTGTTTGAANDWAPSCSTFSTASDKAFALPLPVPVQTLT
                     VDTIGSGFDTVLAIRNAQCSAEIPLACNDDGGGSLTSKIDLSNVAPGNYAIIVDGYNT
                     NNGSFTLNVRGTVAPQTSCTSALFTGGANAVLVCPTGTTCTGSPLKCQ"
     gene            complement(2197..2655)
                     /gene="ybeY"
                     /locus_tag="VFV99_31490"
     CDS             complement(2197..2655)
                     /gene="ybeY"
                     /locus_tag="VFV99_31490"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5063960.1"
                     /translation="MALELSIDDGIAVAPAIKAKLRREVARMVKAAALTERRKDLEVA
                     LRLCDDVTIRELNRDYRNKDKPTDVLAFAQREAATADFALLGDIIISVQTAKRQAKKG
                     LYAELLHLASHGLCHLLGYDHRDDEEERVMNERAASLRREAARTGRVKAA"
     gene            complement(2660..4636)
                     /locus_tag="VFV99_31495"
     CDS             complement(2660..4636)
                     /locus_tag="VFV99_31495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5063961.1"
                     /translation="MASPDDLVNAVSRDRRMQGYRVRRQGDGWIELEVIAQRARSPVL
                     LVLAWPDVWKTPKALQPHLVRARAGNYGLILVGADAEFEQERLDAAPSDADLTTITAP
                     ISMVRLLLQLRQRSEAIAMRMQSAALELELERSRHENDMLISIGRALSQERDIQSLLA
                     IILRRACEVTNADAGSVYIVEGHDEDVSKRTLRFAVSQNESRNVQSAGFTMPISASSI
                     VGTCVLSGDRINVPDLYNLDEPGVGNNPWGFVHDRSFDDKYRYQTRSVLAVPMISART
                     EVIGVIQLINKRAKGWIQLDLPADFEDGVVPFDDVSEAYVGALASQAGIALENVLLYE
                     EVKTLFDSFVKAAVTAIESRDPTTSGHSERVAELTVGLARAVNRAESGHYREVRFSRD
                     DLTQIEYAALLHDFGKVGVREHILVKAKKLYEHERELILQRFQLIKRGFKIEGLEKKV
                     RYLVEASRDQVAAELESVDKDVVAKVTELDNIIQFILAANEPTVLDQGGFERLAELSG
                     MTYRDEAGTALPFLTHDEATCLQIRRGSLTESERLEINSHVVHTFNFLVRIPWSRTLR
                     DVPEIAGAHHEKLDGTGYPNKLKGDQIPVPARMMAISDIYDALTASDRPYKKAMPVEK
                     ALDILSGDVKRGQLDADLFEVFVGARVWALTTRS"
     gene            complement(4646..6769)
                     /locus_tag="VFV99_31500"
     CDS             complement(4646..6769)
                     /locus_tag="VFV99_31500"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5063962.1"
                     /translation="MAKQIHREKLAFDDPEALQALCGSNNSRLKLIERTAGAEVHLRG
                     NEITIEGEEGATETAKSALEQLYDLALAGKPLSSDDVVRAVRLLQGNGDNGQTIATVF
                     GETILTPRAGRPIAPKGATQKKYVDLVRSNDIVFAVGPAGTGKTYLAMALAVRALLDK
                     QVRRIILTRPAVEAGERLGFLPGTLEEKVDPYLRPLFDALHDMMDPERLTRNMADDII
                     EVAPLAFLRGRAQPLSSNVLTPTGWRRIGDLEVGDEVIGSDGWPTRVDGVYPQGTKDV
                     FRVTMTDGASTHCCAEHLWSVYTLEDRRRNKPPRVVDTATMAATLHRHKQCRYELPLL
                     RAPVAFPPQRVPLEPYALGLLLGDGCITGSTTPTFATRDRELVDSLQIGLGERIELVH
                     KAGPDFVLRHRGEHRGGRREANPVTEQLRELGLCGTRSNTKFVPAMYLFNCAEVRLAV
                     LQGLLDTDGGPVTQQGSTCRIQYTTTSPRLRDDVLFLVRSLGGIAYCRTRLAAGRSPG
                     LANGRPVVHRHDAYILDIRLPNYVKPFRLVRKAKLYHEHGSAPPKRFVRRIEPAGREE
                     TRCIRVAAPDSLYVTDDLILTHNTLNESFIVLDEAQNTTPAQMKMFLTRMGYGAKVVI
                     TGDITQTDLPAGQRSGLRDALDLVHDIRGIGTISFTDADVVRHPLVAALIRAYDARDR
                     ARFEAREARENREPQDKGSPDDIDS"
     gene            6796..7587
                     /gene="mazG"
                     /locus_tag="VFV99_31505"
     CDS             6796..7587
                     /gene="mazG"
                     /locus_tag="VFV99_31505"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX5063963.1"
                     /translation="MQPGSSLARLVEIMDRLLGPDGCPWDREQTLETLRPFLVEETYE
                     VLDALSRDDAADHCEELGDLLMQIVFQAALRRDQGAFDIDAVVASISEKLIRRHPHVF
                     ATAEGVDTPEKVLAQWDQIKAAEKAEKGEQKKRVLDGIPEGPALVRAQKIGHRCGKVG
                     FDWPGWEGSFAKVEEEVTEVLGAVRAQDSREVHKEIGDLFLAVVNLSRKLGVDAENAL
                     VDATKRFEQRFAIVEDRLADRGKTPQQSNLEEMDALWNDAKKSVR"
     gene            7811..8068
                     /gene="rpsT"
                     /locus_tag="VFV99_31510"
     CDS             7811..8068
                     /gene="rpsT"
                     /locus_tag="VFV99_31510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5063964.1"
                     /translation="MANIKSAEKQNRKMIKHRARNRAAMASLRTAVKSARTAVDSKAA
                     NAQELVKKAVSIVDSAVTKGILKRQTASRYVSRLASRAHAR"
     gene            complement(8087..9463)
                     /locus_tag="VFV99_31515"
     CDS             complement(8087..9463)
                     /locus_tag="VFV99_31515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5063965.1"
                     /translation="MEHLTGTRIRREATPATVNLRRCKLVVIKGAQRGTEFVISGDVI
                     RIGKAPENDLVLADETVSRVHFEIVRDAKGYLVRDLKSTNGTFLDSAEVKEAYLRAGS
                     VISVGATELKFTPFEERIEILPSEKETLGEMVGKSPAMREIFGLIERIAPTDATVLIE
                     GETGTGKDMIARTLHQLSPRADKPFIVVDCGAVAGTLIESELFGHEKGAFTGATSARQ
                     GAFELASGGTVFLDELGELSLDLQPKLLRVLEQRELRRVGGTKTIKVDLRVIAATRKD
                     LRSEVEKGKFREDLYFRLNVVPITAPSLRERREDIPLLIDHMLTKLAPDAATQLTDTT
                     RAALMAHDWPGNVRELRNVIERALALGADPGMLVAPLGPEATTSSKGVALRDAIEFEA
                     GVSFRDTKEKWNELFERRYLAWLIKRAEGNISKAARDADMDRKYLHKLLRKYGITAQG
                     ESGDDDEN"
     gene            9526..10215
                     /locus_tag="VFV99_31520"
     CDS             9526..10215
                     /locus_tag="VFV99_31520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX5063966.1"
                     /translation="MLRSLGVIALTASVYGGIANAQPAPAKPSDAPLPSCLDQTIAEQ
                     VGEQLKPRGVQKKDFLKRRKINVVLQGGLNGGDLMSSSWIAGGSIGFFFTEDFGFAGE
                     FDVTPLELDLDSPINKFFGDDRFEPGLAYVALGKLFWSPIHAKLKLGGGIVHADFLVF
                     AGAGRMFHDAVQGIAYDAGAAIDMFVTKGMTIRIGLQDLMAVEEIAAETHFTNNLIAT
                     AGLALWIPSGL"
     gene            10212..10940
                     /locus_tag="VFV99_31525"
     CDS             10212..10940
                     /locus_tag="VFV99_31525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX5063967.1"
                     /translation="MKRALVLLAVLAPALAHADDKKQVAAADSKDDKKADKDEAAVGT
                     TSMCIDQEIADRLAIKRKRRGAVDRLFVKQGRHELTAAGGYYESDLLSGTYLVGGSYT
                     YHMTDETAVEFAGWWTHANADLIRALEDKRGVVLTETYARMIFAESLLVWSPVYGKLR
                     LGGTIVHFDLHADLGVGVIDSKTSRGAAGVLGFGIKLFAGQAVAFRIDARNRTFRQDL
                     LEERFLVNDAALTAGFSLFLPFSN"
     gene            10966..11808
                     /locus_tag="VFV99_31530"
     CDS             10966..11808
                     /locus_tag="VFV99_31530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063968.1"
                     /translation="MRRGILIALLVPAVAFAGPKKKKKKAAAPPPPVEEKKEPEPAEE
                     KAAPTPEPPGTPTVPTTTKPGTAPATSGPLTTKPGTPTVPLSGTTAPTGTPAPTPSVA
                     PPKEEEEPPAKEGKDVDVDALRQEYLSLRDELFKSRARANAVASQLYSTRVSIKFSWG
                     SARHYGVSKASIRLDGATVYEDTTGAIANDDGVRFNGFVAPGRHLITFHVEAAGKDDD
                     SFTSSTESQVVVKAVANKDLMISAKGRDNGDIAYEWKRSEKGSYGLGIDVSVKTAAAA
                     GAKK"
     gene            11805..>12855
                     /locus_tag="VFV99_31535"
     CDS             11805..>12855
                     /locus_tag="VFV99_31535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063969.1"
                     /translation="MRLSALVTVALFCAAGSTQAAPGGAKAASESTSGGVEQKSDELA
                     KYFQQLESMKLIDAESGTLETLKRELGIAEELLHSGAYQNAAVALYAIVKSPRYSAFT
                     DFVEYQNAEYDLGVALSRSGSYGAAMDIIEQTLHRGPSAPYWGPAHRRAVDIAIETRD
                     HAGILARIEAIKTTDPIPPSAAGERAYLRGRSAYETGKLADAEAELISISRKSRLYSS
                     AVYLRGVIRARRGQYKEAAEAMCEIAATPDTDKITFVVDDRYFTVKDLARLGLGRIAH
                     EQSEYDDAYYHYFQIPEDSTYLPDALFEAAWSMYQKRELATSRDLVKEFLKEFPSSPL
                     WPEASLLAGYVELADC"
BASE COUNT         2340 a         4195 c         4131 g         2189 t
ORIGIN      
        1 gaaccaggat gacgagaccg acgactgtcc agacggtccg agctgcccgc agtgctcgaa
       61 caacaaggat gacgacggga acggccagac cgactacccg aacgactcgg gaggctgcgc
      121 gtccgcgtcc gacaccgacg agtacacgcg taatccggtc gcgtgcggca gcaacgtgat
      181 gatcaagatc ctgccgttcg acggccatgc gatgggcacc atcggcaaca tgggcgcgtc
      241 gagcctcgtg tcgccgacgt gcggcggcgc tggcctcgaa gatgtctacg agctgcgcgt
      301 cacgtcgccg aaggtaatcg tcgcttcgac cgatagcgat gcgaccggca tcgataccgt
      361 gctctacatc cgcggctctg actgcgcgaa catgtcgagc gagatggtct gcaacgacga
      421 catctcgacc acggacaaga agtcgactgt caccaagtcg atcacgacgc cgggcacgta
      481 ctacctggtc gtcgacgcgc acgacacgag cgtcagcggc tcctacgacc tgacggtgaa
      541 gttcctcacc ggcgaaggcg agacgtgcag cggcgccgac gattgcggtc ccggcctcgt
      601 ctgccgcatt cccggcggcg gcacgcagaa gatctgcacg aagcacgtct gcgaagacaa
      661 catcgacgac gacatggacg gcaagctcgg atacccgacc gatccgggtt gctcgagcgc
      721 gaccgacgat gacgagaccg acggctgccc cggtgtcggt ccgaactgcc ccgagtgcgg
      781 tgacggcgtc gacaacgatg gcgacggcaa gaccgactac ggcacgaacg gcgacatgac
      841 ctgcacgtcc gctgccgacg cgagcgaagc atgcgtgagc caggaaggcg tccaggcgat
      901 cacgacggcg atgacgatgg gtgacaccac cggccagcac aacgatgtcg tgccgtcctg
      961 cggcagcacg accagcaccg cgccggaccg cacctaccgc ctcgacgtcc cggcgatggc
     1021 gaacctccag ctcaacctga cgacgagctt cgataccgtg accgcgctct acaactcgac
     1081 gtgcggcggc accgcggtgg cgtgcagcga tccgaccacc atgagcgtca acaacctcgc
     1141 ggccggcacg tactacttcg tcgtcgacgg ttacttcagc acgtcggtcg gcacctacac
     1201 gatcaacgtc ctcggcaaga tccagaacgg ccagagctgc gagagcgcgc tggcacaatc
     1261 gggcgcgatc acctgcaaca cgggctacgc gtgcaagggc acgatggggt cgcgtacctg
     1321 ccaaccggca ctgtgctcgg atggcctgga caacgacacc gacggcaaga tcgactaccc
     1381 gaacgacccg ggctgcgtcg actcggccga cgacaccgag cagaacccga ccacgctgcc
     1441 ggtctgcgcc gacggctcgg acaacgatac cgacggtctc accgacttcc cggcggacta
     1501 cggctgcgcg gccgcgagcg gcacgagcga agtgttctgc atgggcgaga tggacacgac
     1561 cgcggcgatc gcgacgaaga ccacgacggg caccacgacc ggtgccgcga acgactgggc
     1621 gccgtcgtgc agcacgttct cgaccgcatc ggacaaggcg ttcgcgctgc cgttgccggt
     1681 gccggtccag acgctcaccg tcgacaccat cggctcgggc ttcgacaccg tgctcgcgat
     1741 tcgcaacgcg cagtgctcgg cggaaatccc gctggcgtgc aacgacgatg gcggcggcag
     1801 cctgacgtcg aagatcgacc tctcgaacgt cgctcccggc aactacgcga tcatcgtcga
     1861 cggctacaac acgaacaacg gctcgttcac gctcaacgtc cgcggcaccg tggcgccgca
     1921 aacgtcgtgc acgtcggcgc tgttcaccgg cggcgcgaac gcggtgctcg tgtgcccgac
     1981 cggcacgacg tgcacgggct cgccgctgaa gtgccagtag cccgccggag gtaaccgtcg
     2041 ccgccgcaaa cgccaaggtc ggaaactcga tctttatcgc gctccgcaca cgcacgcgcc
     2101 ggcgccgatc gcgaagggcg cggcgtctgc ccagcggctc gccggaggtc cgcttcggtt
     2161 tttgctctga gcgtcttggc ggttgatctc ttcgagctac gccgccttta cacgacccgt
     2221 ccgtgccgct tcgcgacgga gcgaggcggc ccgttcgttc atcacgcgct cttcttcgtc
     2281 gtcgcggtgg tcgtagccga gcaagtggca gaggccgtgt gacgcgaggt gaagcagctc
     2341 ggcgtaaagg cccttcttcg cctgccgctt ggcggtctgc accgagatga tgatgtcgcc
     2401 gagcagcgcg aaatctgcgg ttgccgcctc gcgctgcgcg aatgcgagca cgtcggtcgg
     2461 cttgtccttg ttgcggtagt cgcggttgag ctcgcgaatc gttacgtcgt cgcataggcg
     2521 caacgcgacc tcgaggtcct tgcggcgctc cgtgagcgcg gcggccttca ccatgcgcgc
     2581 aacctcgcgg cgcagcttcg ccttgatggc gggtgcgacg gcgatgccgt catcgatcga
     2641 gagctcgagc gccatgccgt cagctacgcg tagtgagcgc ccacacgcgc gcgccgacga
     2701 acacttcgaa caggtcggcg tcgagctggc cccgcttgac gtcgcccgaa agaatgtcga
     2761 gcgccttctc gaccggcatc gccttcttgt acgggcggtc gctcgcggtc aacgcgtcgt
     2821 agatgtcgct gatcgccatc atgcgggcgg gtactgggat ctggtcgccc ttgagcttgt
     2881 tcggatagcc ggtgccgtcg agcttttcgt ggtgcgcgcc cgcgatctcg gggacgtcgc
     2941 gcagcgtgcg gctccacggg atgcggacga ggaagttgaa cgtgtggacg acgtggctat
     3001 tgatctcgag acgctcggac tcggtgagcg agccgcgacg gatctggagg cacgtcgctt
     3061 cgtcgtgcgt gaggaacggt agcgccgtgc cggcctcgtc acggtaggtc atgcccgaca
     3121 gctcggcgag gcgttcgaag ccgccctggt cgagcaccgt cggctcgttc gcggccagga
     3181 tgaactggat gatgttgtcg agctcggtaa ccttggcgac cacgtccttg tcgacggact
     3241 cgagctcggc ggcgacctga tcgcgcgacg cctcgacgag gtagcgcacc ttcttctcga
     3301 ggccctcgat cttgaagccg cgcttgatga gctggaagcg ctgcaggatc agctcccgct
     3361 cgtgctcgta gagcttcttc gctttgacga ggatgtgctc tcgaacgccg accttgccga
     3421 agtcgtgcag cagcgccgcg tactcgatct gcgtgaggtc gtcgcgcgag aagcgcacct
     3481 cgcgatagtg gccgctctcg gcgcggttga cggcgcgcgc gaggccgacc gtgagctcgg
     3541 cgacacgctc ggagtgaccg gacgtcgtcg gatcgcgcga ctcgatcgcg gtaacggccg
     3601 ccttcacgaa gctgtcgaac agcgtcttga cctcttcgta gagcagcacg ttctcgagcg
     3661 cgatgccggc ttgcgatgcg agcgcgccga cgtaggcctc ggaaacgtca tcgaacggca
     3721 cgacgccgtc ctcgaagtca gcgggcagat cgagctggat ccagcccttc gcgcgcttgt
     3781 tgatgagctg gatgacgccg atcacctcgg tgcgcgcaga gatcatcggc accgcgagca
     3841 cggagcgcgt ctggtagcgg tacttgtcgt cgaagcttcg atcgtggacg aagccccacg
     3901 ggttgttgcc gacgccaggc tcgtcgaggt tgtagaggtc cggcacgttg atgcgatcgc
     3961 ccgacagcac gcaggtgccg acgatcgacg atgccgagat gggcatcgtg aagccggccg
     4021 actgcacgtt gcgcgactcg ttctgcgaca ccgcgaatcg cagcgtgcgc ttcgagacgt
     4081 cctcgtcgtg cccctcgacg atgtacacgc tgccagcgtc ggcgttcgtg acctcgcagg
     4141 cgcggcgcaa gatgatcgcg agcagcgact ggatgtcgcg ctcctgcgat agcgcgcggc
     4201 cgatcgagat cagcatgtcg ttctcgtggc gcgagcgttc gagctcgagc tcgagcgcgg
     4261 ccgactgcat gcgcatcgcg atcgcctccg agcgctgacg cagctgcagc aacaggcgca
     4321 ccatcgagat cggcgccgtg atcgtcgtca gatcggcgtc actcggcgca gcgtcgaggc
     4381 gctcctgctc gaactcggca tcggcgccca ccaggatgag gccgtaattg cccgcacgcg
     4441 cgcgcacgag gtgcggttgc agcgccttcg gggttttcca cacgtcgggc cacgcgagca
     4501 cgagcagcac cgggctgcgc gcgcgctgcg cgatcacttc tagctcgatc cagccatcgc
     4561 cctgacgccg cacgcgataa ccctgcatcc gccggtcacg cgataccgcg ttgaccagat
     4621 cgtctgggct cgccaccacc attgactacg agtctatatc gtcgggcgaa cctttgtctt
     4681 gaggctcgcg gttctcgcgt gcctcgcggg cctcgaaccg agcgcgatca cgcgcgtcgt
     4741 aggcgcggat cagcgctgcg acgaggggat gtcgcaccac atctgcgtcg gtaaaggaaa
     4801 tcgtgccgat gccacggatg tcatggacca ggtccagtgc gtcgcgcaga ccactgcgct
     4861 ggccggccgg caggtcggtc tgcgtgatgt cgccggtgat gacgaccttg gccccgtagc
     4921 ccatacgggt gaggaacatc ttcatctgcg ccggcgtcgt gttctgcgcc tcgtcgagca
     4981 cgatgaacga ctcgttgagc gtgttgtggg tgaggatcaa gtcgtccgtg acgtacagcg
     5041 agtcgggggc cgccactcga atacagcgcg tctcttctct accggcaggt tcgatcctac
     5101 gtacaaaacg cttggggggc gcgctgccat gctcgtggta cagcttggcc ttacgaacaa
     5161 gtcggaacgg cttcacgtag ttcggtagcc ggatgtcgag aatgtaagca tcgtgccgat
     5221 gaacgaccgg tcgcccattc gctaggcccg gtgaccgacc cgcagctagt ctcgttcggc
     5281 agtaggcgat gccccccaac gaacgcacga gaaacaagac atcgtcgcga agccgcgggg
     5341 acgtagtcgt gtactggatc cggcatgtgc tgccctgctg cgtaactggc ccgccgtcgg
     5401 tatcgaggag accctgcaac accgcgagcc gtacttcggc gcagttgaac aggtacatcg
     5461 ctggcacgaa cttcgtgttc gaacgcgtgc cgcagagccc cagctcgcgg agctgttcgg
     5521 ttaccgggtt cgcctcacgt cgtccgccgc ggtgctcgcc tcggtgtcgc agtacgaagt
     5581 ctggtcccgc cttgtgcacc agctcgatgc gctccccgag gccgatctga agcgaatcga
     5641 cgagctcccg atcacgagtt gcgaatgttg gcgtggtcga tccggtgatg catccatcac
     5701 cgagtagaag gcccagcgcg tagggctcga gcggtacgcg ttgcggcgga aaagctactg
     5761 gtgcacgcag caacggcagc tcgtagcgac actgcttgtg gcgatgcagc gtcgcggcca
     5821 tcgtggccgt atcgacgaca cgtggcggct tgttgcgtcg gcgatcctca agcgtgtaga
     5881 ccgaccagag gtgctcggca cagcaatgtg tcgatgcccc atcggtcatc gttacgcgga
     5941 acacgtcctt cgtaccctgc gggtacacgc catcgacgcg agttggccaa ccatcagatc
     6001 cgatgacctc atctccaacc tccagatccc cgatccgacg ccaacctgtt ggggtgagaa
     6061 cgttgctcga aagcggctgg gctcgaccgc gcaagaacgc gagcggcgcg acctcgatga
     6121 tgtcgtcggc catgttccgc gtcagcctct cgggatccat catgtcgtgg agcgcgtcga
     6181 acagcgggcg cagatacgga tcgaccttct cctcgagcgt gccgggcaag aagccgagcc
     6241 gctcgccggc ctcgacggcg ggacgcgtga ggatgatccg gcgaacctgc ttgtcgagca
     6301 gcgcgcgcac cgcgagcgcc atcgcgagat aggtcttgcc ggtaccggcg ggaccgaccg
     6361 cgaacacgat gtcgttcgag cgcacgaggt cgacgtactt tttctgcgtc gcgcccttcg
     6421 gtgcgatcgg ccgacccgca cgcggcgtca ggatcgtctc gccgaacacg gtcgcgatcg
     6481 tctgcccgtt gtcgccgttg ccctgcagca agcgcaccgc gcgcacgacg tcgtccgacg
     6541 acagcggctt accggcgagc gcgaggtcgt agagctgctc gagcgcggac tttgcagtct
     6601 cggtcgcgcc ctcctcgcct tcgatcgtga tctcgttgcc gcgcagatgc acctcagccc
     6661 ctgccgtacg ctcgatcagc ttgagccggc tgttgttgct cccacacagc gcctgcagcg
     6721 cctcgggatc atcgaatgcg agtttctcgc ggtgaatctg cttggccacg acgaagacgg
     6781 cggtatagca cgcatatgca gcctggtagc tcgctagccc gactggtcga aatcatggac
     6841 cgattgctcg gcccggacgg ctgcccgtgg gaccgcgagc agaccctcga gacgttgcgg
     6901 ccgttcctcg tcgaggagac ctacgaggtc ctcgacgcgc tgtcgcgcga cgacgccgcc
     6961 gatcattgcg aagagctcgg cgacctcctc atgcagatcg tgtttcaagc ggcgctgcgc
     7021 cgcgatcaag gcgcgttcga catcgatgcg gtggtcgcca gcatctccga gaagctgatc
     7081 cgccgccatc cgcacgtgtt cgcgactgcc gaaggcgtcg acacgccaga aaaagttctc
     7141 gcgcagtggg accagatcaa agccgcggag aaagccgaga aaggcgaaca gaagaagcgc
     7201 gtcctcgacg gcattccaga aggccctgcc ctcgtgcgcg cgcagaagat cggccaccgc
     7261 tgcggcaagg tcggtttcga ctggccaggt tgggaaggct cgtttgcgaa ggtcgaagaa
     7321 gaagtgaccg aggtgctcgg cgcagttcgc gcacaagatt cacgcgaagt gcacaaagag
     7381 atcggcgacc tattcctcgc ggtcgtgaac ttgagtcgaa agctcggcgt cgatgcagag
     7441 aatgcgctcg tcgatgcgac aaaacgtttc gagcaacggt ttgcgatcgt ggaagaccga
     7501 ctcgccgatc ggggcaagac gccacagcaa tcgaacctcg aagagatgga cgcgctgtgg
     7561 aacgacgcga aaaaatctgt gcgatgaaag tgactgtttt ttgagcagtc gagatcgagt
     7621 cgaagagatc tcgacatgat caccctctcg cgcccccatc cctgaacaac ttatccacag
     7681 aaccggtgtg tactcccgca atgttcgtgg gtgaattcga tgcattgggg ccgcgaacaa
     7741 gcctcaactg ccagggcttg acaccccctc ccggggcggg gtaaacgctc atccctccag
     7801 gagcgcctac gtggccaata tcaagtcagc cgagaagcaa aaccgtaaga tgatcaagca
     7861 tcgcgcgcgc aaccgcgccg cgatggcgtc gctgcgtacc gccgtgaagt ctgcccgcac
     7921 cgccgtcgac agcaaagctg ccaacgcgca ggagctcgtg aagaaggccg tgtcgatcgt
     7981 cgacagcgcc gtcacgaagg gcatcctcaa gcgccagacc gcgtcgcgtt acgtgtcgcg
     8041 tctcgcgtcg cgcgcccacg ctcgctaagc ggtctcgcgc ggctcgctag ttttcgtcgt
     8101 cgtcgccgct ctcaccctga gcggtgatgc cgtacttccg taacagttta tggaggtact
     8161 tgcgatccat gtccgcgtcg cgcgcggcct tggagatgtt gccctccgcg cgcttgatca
     8221 gccacgcgag gtagcggcgc tcgaacagct cgttccactt ctccttcgtg tcgcggaacg
     8281 agacgcccgc ttcgaattcg atcgcatcac ggagcgcgac gcctttgctg ctggtggtcg
     8341 cctcggggcc gagcggcgcg accaacatgc ctggatcggc gccgagcgcg agtgcccgct
     8401 cgatcacgtt gcgaagctcg cgaacgttgc ccggccagtc gtgcgccatc agcgccgcac
     8461 gcgtcgtgtc ggtgagctgt gtcgcggcgt cgggcgcgag cttggtgagc atgtgatcga
     8521 tgagcagtgg aatgtcctcg cggcgctcgc gtagcgaggg cgccgtgatc ggtacgacgt
     8581 tgagccggaa gtagaggtcc tcgcggaact tccccttctc gacctccgaa cgcaggtctt
     8641 tgcgcgtcgc agcgatgaca cgcagatcga ccttgatcgt cttcgtaccg ccgacgcgac
     8701 gtagctcgcg ctgctcgagc acgcgcaaca gttttggctg caggtcgagc gacagctcgc
     8761 cgagctcgtc gagaaacacg gttccgccgg acgcgagctc gaacgcgcct tgccgcgcgc
     8821 tcgtcgcacc cgtgaacgcg cccttctcgt ggccgaacag ctcgctctcg atcagcgtgc
     8881 ccgcgacggc gccgcaatcg acgacgatga atggcttgtc agcgcgcggc gacagctgat
     8941 ggagtgtgcg cgcgatcatg tctttgccag taccggtctc gccttcgatc agcaccgtcg
     9001 cgtccgtcgg cgcaatacgc tcgatcaggc cgaagatctc ccgcatcgcg ggcgacttgc
     9061 cgaccatctc gccgagcgtc tccttctccg acgggaggat ctcgatgcgt tcttcgaatg
     9121 gcgtgaactt gagctcggtg gcaccgacgc tgatcacaga gccagcgcgc aggtatgcct
     9181 ccttgacctc ggcgctgtcg aggaacgtgc cgttcgtcga tttgagatcg cgcacgaggt
     9241 aacccttcgc atcgcgcacg atctcgaaat gtacgcgcga cacggtctcg tctgcgagca
     9301 cgagatcatt ctctggagct ttgccaatgc ggattacgtc tccggagatc acgaactcgg
     9361 tgccgcgttg tgcccctttg atgacaacga gcttgcagcg acgaaggttg accgtcgccg
     9421 gcgtcgcctc acgccggatc cgggtccccg tcaaatgctc catcggcgga atcgtaccat
     9481 gcgcaacgtg cgccctagcc tgagcttgtt gtacgctcgg gtctagtgct gcgcagtctt
     9541 ggggtgatcg cgctgacggc cagtgtttat ggcggtattg ccaacgcgca acctgcgccg
     9601 gcaaagccgt cggacgcgcc gcttccatcg tgtctcgatc aaacgatcgc cgagcaggtt
     9661 ggcgagcagc tcaagccgcg cggcgtacag aagaaggact tcctcaagcg ccgcaagatc
     9721 aacgtcgttc tgcaaggcgg tctgaacggc ggcgacctga tgtcgtcgag ctggatcgcc
     9781 ggtggctcga ttggcttctt cttcaccgag gacttcgggt tcgccggcga attcgatgtc
     9841 acgccgctcg agctcgatct cgactcgccg atcaacaagt tcttcggcga cgatcgcttc
     9901 gagccgggcc tcgcgtacgt cgcgctcggc aagctgttct ggtcgccgat ccacgccaag
     9961 ttgaagcttg gcggtggcat cgtgcacgcc gacttcttgg tcttcgccgg tgccggccgc
    10021 atgttccacg acgccgtgca gggcatcgcc tacgacgcag gcgccgcgat cgacatgttc
    10081 gtcaccaaag ggatgacgat ccggatcggc ctgcaagacc tcatggccgt cgaggagatc
    10141 gcagccgaga cgcacttcac caacaacctg atcgcgaccg ctgggctcgc gctgtggata
    10201 ccgagcggac tatgaaacgt gccctcgttc tccttgcagt cctcgcgccg gcgctcgcgc
    10261 atgccgatga caagaagcag gtcgcggccg ccgattcgaa ggacgacaag aaagcagaca
    10321 aggacgaggc agccgtcggc acgacgtcga tgtgcatcga tcaagagatc gcggaccgac
    10381 tcgcgatcaa gcgcaagcga cgcggtgcag tcgaccgcct gttcgtgaag caaggccgtc
    10441 acgagctcac ggcggccggt ggctattacg agagcgactt gctctccggc acatacctcg
    10501 tcggcggctc gtacacgtat cacatgaccg acgagaccgc ggtcgagttc gcgggttggt
    10561 ggacgcacgc gaacgcagat ttgatccggg cactcgaaga caagcgcggt gtggtgctta
    10621 ccgaaacgta cgcgcgcatg attttcgcag agtcgctgct ggtgtggagc cccgtgtacg
    10681 gcaagctgcg actcggagga acgatcgtcc atttcgatct gcacgcggac cttggcgtcg
    10741 gcgtgatcga ctcgaagacg agccgcggcg ccgccggcgt cctcggtttc ggcattaaac
    10801 tatttgcggg gcaagcagtc gccttccgga tcgatgcacg taaccgcacg ttccgacagg
    10861 acctgctcga agaacgtttt ctcgtcaacg atgctgcgtt gactgcgggc ttctcgctgt
    10921 ttctgccgtt ctctaactag tcgccgtgtc acgctcggag agatcatgag aagagggatc
    10981 ttgatcgcgc tgctcgtgcc tgcggttgca ttcgcgggcc cgaagaagaa aaagaagaag
    11041 gcagctgcgc ctccacctcc ggtcgaggag aagaaggagc cggaaccggc tgaggagaaa
    11101 gcggcgccga cgcccgagcc tcccggcaca ccgacggtcc cgaccacgac gaagcccggc
    11161 acggcgcccg cgacctcggg cccgctgaca accaagccgg gtacaccgac ggttccgctc
    11221 agcggcacga ccgcaccgac cggcacgccc gcaccgacgc cgagcgtggc accgcccaag
    11281 gaagaggaag agccacccgc gaaggaaggc aaggacgtcg acgtcgatgc gctgcgccaa
    11341 gagtacctgt cgctgcgcga cgagctcttc aagtcgcgcg cgcgtgcgaa cgccgtcgcg
    11401 agccagctgt actcgacgcg cgtcagcatc aagttctcgt ggggctcggc gcgccactac
    11461 ggcgtgtcga aagcgtcgat tcgcctcgat ggtgcgaccg tgtatgaaga cacgaccggc
    11521 gcgatcgcca acgacgacgg cgtgcggttc aacggcttcg tcgcacctgg tcgtcacctg
    11581 atcacgttcc acgtcgaagc cgccggcaag gacgatgact cgttcacgtc gtcgacggag
    11641 tcacaggtcg tcgtcaaagc cgtcgcgaac aaagacctca tgatctcggc gaagggccgc
    11701 gacaacggtg acatcgcgta cgagtggaag cgcagcgaga aaggcagcta cggcctcggc
    11761 atcgacgtct cggtgaagac ggccgcagca gcgggggcga agaagtgagg ctctccgcgc
    11821 tcgtcacggt cgcgctgttc tgcgccgcag gctctacgca agcagcgccc ggtggagcga
    11881 aggccgcgag cgaatcgacg tcgggtggcg tcgagcagaa gagcgacgag ctcgcgaagt
    11941 acttccaaca gctcgagtcg atgaagctca tcgacgccga gagcggcacg ctcgagacgc
    12001 tcaagcgcga gctcggcatc gccgaggagc tattgcacag cggcgcgtac cagaacgctg
    12061 ccgtcgcgct ctacgcgatc gtgaagtctc cgcgctactc cgcgttcacg gacttcgtcg
    12121 agtaccagaa cgccgagtac gacctcggtg tcgcgctctc gcggtctggc agctacggcg
    12181 ccgcgatgga catcatcgag cagacgctgc accgcggacc gagtgcaccg tactggggcc
    12241 ccgcacatcg ccgcgccgtc gatatcgcga tcgaaacccg cgaccacgcc ggcatcctcg
    12301 cccgtatcga ggcgatcaag accacggatc cgattccgcc gtcggccgct ggcgagcgcg
    12361 cgtacctgcg cggccgctcc gcgtacgaga ccggcaagct cgccgatgcc gaagcggagc
    12421 tgatctcgat ctcgcgcaag agccgcctgt actcgtcagc ggtgtacctg cgcggggtca
    12481 tccgcgcgcg gcgcggtcag tacaaagagg ccgccgaggc gatgtgcgag atcgcagcga
    12541 cgcccgacac cgacaagatc acgttcgtcg tcgatgatcg ctacttcacc gtcaaagacc
    12601 tcgcgcgcct gggtctcggc cggatcgcgc acgagcagag cgaatacgac gacgcctact
    12661 accactactt ccagattccc gaggactcga cgtacctccc cgacgcactg ttcgaagcgg
    12721 cgtggtcgat gtaccagaag cgcgagctcg cgacgtcgcg cgacctcgtg aaggaattct
    12781 tgaaagagtt cccgtcatcg ccgctgtggc cggaagcgag cttgctcgcg ggctacgtcg
    12841 agctcgccga ctgcg
//
LOCUS       DASUFX010000780         7362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1236970, whole genome shotgun sequence.
ACCESSION   DASUFX010000780 DASUFX010000000
VERSION     DASUFX010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236970"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..459)
                     /gene="cyaY"
                     /locus_tag="VFV99_31540"
     CDS             complement(142..459)
                     /gene="cyaY"
                     /locus_tag="VFV99_31540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX5063970.1"
                     /translation="MDEATFDHEARAELRRIEDAFADIDPDDVEVSTSDGVLRLDLRD
                     GTKIVINSHRAARQIWMAAVASAWHFDPTDSGTWIAAKSGDELRATLARLVKDRIGLE
                     LHV"
     gene            498..1973
                     /locus_tag="VFV99_31545"
     CDS             498..1973
                     /locus_tag="VFV99_31545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063971.1"
                     /translation="MGTPSDLELDIPEPVEREGHETLLPSVTRSMLSTKSFVSDIEGP
                     TPSATGSNVAPSIEDAVRTEAGRVRGARSGDIVGGRYVVEGQLGRGGMGRVLRVRHSA
                     LGKPFALKLIKSAIATNAKIRELFYREARLASALTHDNICSIVDFGQDEAFGLFMVME
                     LLEGQTVHARLRHSGGFTAKVACDVMWQVSDAARFIHSRQILHGDIKTENIFLVRTPA
                     QRRLIKLLDFGLSRADLGHTEGVDGTPEYLAPERIDGAPASQASDIYAMGIVFFELLT
                     GKLPFVGDTKEVFKQHKKYAVPLASQYVEGEALDERADQIIARATAKDPADRHPDVAA
                     FMYELRTLMGMLGMEQGQGGRRRAVAPDAQRERREYDHRLKAAAEVFGAVPLPMACCD
                     PEGRVRAANPAFLEFLGCGGLASGLQLRDSALPEVYPTLFEDLETVATSRRPIKRVIY
                     LNEGGDRVIEAAVVLSAAPSAAEVTAGEVYVVLHPLRSLLS"
     gene            complement(1980..3410)
                     /locus_tag="VFV99_31550"
     CDS             complement(1980..3410)
                     /locus_tag="VFV99_31550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5063972.1"
                     /translation="MRGSSLVAVIAILFGFGIADASPKSDIQTKIKEAMENYDLMDYD
                     AAKKLLNQALQIAKKNKLDKDPGTAKAHLDLGIVQFVNNDADGAKLSFLSAVQIDPKI
                     QIDAAYKSNEMSKLLEQARKESSGGGGASEPVEATPPPVDTSGVDCSTVQGMQHTIID
                     TAKGGAPLAVEAYLGADVAGTTKVSILYRAEGATDFTEVKMKKDGDCKYTGSIPAAAM
                     KGSLVHYYVAAYADGPKPVASKGSSGSPNIIEISGVAAGGSGDNEDPIGGGGGGGGGG
                     GGSVSSNVTVGPKRARVMIALAGGAGSGYVVGQTEGDGMTHNEVKNCCFGNPIVAVQP
                     EIGYFVNPRLSVGLAARIGFPLGANVEGHATIAPAGLARVRYSLSPSGDGLRVMGQLG
                     VGILRNTIKLDNAMPGMGDTDIVAQGPLLIGAGVGFTRRLSGNIAVMADFSALGAIAV
                     VKELGAAPLLNNGFGADLSLGIQLGF"
     gene            complement(3582..4631)
                     /locus_tag="VFV99_31555"
     CDS             complement(3582..4631)
                     /locus_tag="VFV99_31555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5063973.1"
                     /translation="MKSLVLALCVLVGCAGQQVRSKTTDTVGLIATARDNGAMKCAPV
                     ELAMAESYNDFAIHALDEGNYFDGRRTADIAEKNAKLAIEKSPKDKCNPSGPPPVGDR
                     DRDGLKDDVDKCPDDPEDKDGFEDDDGCPDLDNDADGIKDVVDKCPMDPEDLDKFEDE
                     DGCPDLDNDKDGLADKVDQCPNEPEDKDGFEDDDGCPDLDNDNDTFLDKDDKCPLEFG
                     VAPDGCPKKYVNVVVTKTKIEIKQTIFFEFNKATIKPVSFPLLNEVAQAMQDNPTIKV
                     EVQGHTDSVGNDAFNMKLSQKRAESVKAYLAKRGVSADRMVPKGYGETVPIADNRTAD
                     GRSQNRRVEFVITER"
     gene            complement(4631..5026)
                     /locus_tag="VFV99_31560"
     CDS             complement(4631..5026)
                     /locus_tag="VFV99_31560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063974.1"
                     /translation="MRPWVIALALLSGCGPIQYIKQVPYDAADKVERARSLDAEKYSP
                     YWWTRAVVYLHMAREVAGHADYQGANHFGRLSSEAAEKAAEESAAAASDPSKRPVNPT
                     QELAPAKDDGSTAPGSSSATGGGVAPAKE"
     gene            complement(5040..5603)
                     /locus_tag="VFV99_31565"
     CDS             complement(5040..5603)
                     /locus_tag="VFV99_31565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063975.1"
                     /translation="MRRACAIPVVALINLLFGIGFALIARDRIKADGPFASPAFPLVA
                     MHAAAVVAPIALYFYAVHPAWAWMYWVDPAKLATIFVLPLMVGHAALVIGGWYISAFL
                     IRKGLAGALVYAAGALALFMLVLVAVGINRLSTAADYPGYAAGAGMSLFAVKLGWAFV
                     VSLFALFGSAIYVAIELGRDGRRVRSR"
     gene            complement(5642..>7362)
                     /locus_tag="VFV99_31570"
     CDS             complement(5642..>7362)
                     /locus_tag="VFV99_31570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5063976.1"
                     /translation="IRSLTPFHTVDLLVLREGEQAYVERQGSVRVLRVPTHDADLRAQ
                     IQSYQRALKRQLEGADYDVVHCRDSWSCIPVLEARSRLGYAVVYDMTRSPLGETTLDP
                     ELDAQYGRDEEACILASDLVLAPTPAAVKALQGRGKPERVLLSPPGVDVDRFDWDEAT
                     KSGPPRILYTGSIDPGRGVRVLIRAMAAIVREVDARLVIAGPVIAPKFETALRDGIRE
                     LGLTEKVEMVGAVDHDQLPALIATATVCVVPAAADLTANPAVVFPTKMLEYMACRRAI
                     VAPRRNTVQLVVENGREALLFEPGDPVDLARKVLRLLGEPQLRDRLANAAYERVRHDF
                     TASSARRALRKAYTIIAERFADQFSEEAEDDDAPKIEMLADDDFEATVFEEAPQAPPV
                     DTALNQIEDPESLDELARGHTSSNSVVAPPPPADQTMERHPVAPPPMPPIPKPKQRAD
                     SEAWTQSALTSPPAGQDDWVVTNVAQAVRAPDDDNQETGAVNDDGTPVEGVTVVPPGG
                     TISSENAFVAGEIDVPTPAPEGADAKTPKVEFTAASKLLGNAEQPDPDTGSRTPPLEA
                     VPSRSR"
BASE COUNT         1196 a         2469 c         2384 g         1313 t
ORIGIN      
        1 ggtcaagtcg gcgctgcgaa tcagccggcg cataccctct gcctcggcct cggcgagcat
       61 cccatcggcc catgccacgg cagcccacac ccggatcaca ctcagaatct gcgactcggc
      121 catgcccgca aagatacacg attagacgtg tagctcgagg ccgatccggt ctttgaccag
      181 gcgcgcgagt gtggccctta gctcatctcc gctcttcgcg gcaatccacg taccggaatc
      241 ggtcggatcg aagtgccatg ccgatgccac ggcggccatc cagatctgcc gggcggcccg
      301 atggctgttg attacgatct tcgtgccatc gcgcagatct aggcgtaaca cgccatcact
      361 cgtagacact tcgacgtcat cgggatcgat gtcggcaaat gcgtcctcga tacgccggag
      421 ctcggcgcga gcctcgtgat cgaaggtagc ttcgtccatg cgcaccttat agcgtttttt
      481 cggtactatt gaggccaatg ggtactccgt cggacctgga gctcgacatt cccgagcctg
      541 tggagcgtga aggtcacgag acgttgttgc cctcggtcac tcgctcgatg ctctccacga
      601 agagcttcgt cagcgatatc gaaggcccga cgccctcagc gactggctcc aacgtcgcgc
      661 catcgatcga agacgcggtc cgcaccgaag ccggtcgcgt acgcggagca cgatccggcg
      721 acatcgttgg cggtcgctat gttgtcgaag gccagctcgg ccgcggcggc atgggccgcg
      781 tgctgcgcgt gcgtcactcg gcgctcggca aaccgtttgc gctcaaacta atcaagtcag
      841 cgatcgcgac gaacgcgaag attcgcgaat tgttctatcg cgaagcccgc ctcgcatcgg
      901 cgctgaccca cgacaacatc tgctcgatcg tcgacttcgg ccaggacgag gccttcggcc
      961 tcttcatggt catggagctc ctcgaaggtc agaccgtcca cgcgcgcctg cgccacagcg
     1021 gtggcttcac tgcgaaggtc gcgtgtgacg tgatgtggca agtgagcgac gcggcgcgct
     1081 tcattcatag ccgccagatc ctccacggcg acatcaagac cgagaacatc ttcctcgtcc
     1141 gcacaccggc gcagcgccgc ctgatcaagc tcctcgactt cggtctgtcc cgcgccgacc
     1201 tcggccacac cgagggcgtc gacggcacgc ccgagtatct cgcgcccgag cgcatcgacg
     1261 gtgcacccgc ttcgcaggcg agcgacatct acgcgatggg catcgtgttc ttcgagctgc
     1321 tcaccggcaa gctgccgttc gtcggcgaca cgaaggaagt cttcaagcag cacaagaagt
     1381 acgcggtgcc gctcgcaagc cagtacgtcg agggcgaagc gctcgacgag cgcgccgatc
     1441 aaatcatcgc gcgcgcgacc gcgaaggatc cggccgatcg tcatcccgat gtcgccgcgt
     1501 tcatgtacga gctgcgcacg ctgatgggca tgctcggcat ggagcaaggc cagggtggcc
     1561 gtcgccgcgc cgttgcgccc gacgcgcaac gcgaacgtcg cgagtacgat caccgcctca
     1621 aggccgccgc cgaagtgttc ggcgcagtgc cgctgccgat ggcgtgctgc gaccccgaag
     1681 gtcgcgtgcg cgcggcgaac ccggcgttcc tcgagttcct cggctgcggc ggtctcgcat
     1741 cgggtctgca gctgcgtgac tcggcgttgc ccgaggtcta tccgacgctg ttcgaggatc
     1801 tcgagacggt cgcgacgtca cggcgtccga tcaagcgcgt catctatctc aacgagggcg
     1861 gcgatcgcgt gatcgaagcg gccgtcgtgc tatcggccgc gccgagcgca gccgaagtga
     1921 ccgcaggcga ggtgtacgtc gtgttgcacc cgctgcgctc gctgctgtcc tagcccggct
     1981 tagaagccga gctggatgcc gagcgagagg tccgcgccga agccgttgtt gagcagcggc
     2041 gctgcaccga gctccttcac gaccgcgatc gcaccgagcg ccgagaaatc ggccatcacg
     2101 gcaatgttgc ccgagagccg gcgcgtaaag ccgacgcccg cgccgatgag cagcgggccc
     2161 tgcgcgacga tatcggtgtc gcccatgccc ggcatcgcgt tgtcgagctt gatggtgttg
     2221 cgcaggatgc cgacgcccag ctggcccatg acgcggaggc cgtcaccgct cggagacaac
     2281 gagtaacgca cgcgcgcgag gccggcgggc gcgatggttg cgtggccctc gacgttggcg
     2341 ccgagcggga agccgatgcg cgcggcgagg ccgaccgaca ggcgcgggtt cacgaagtaa
     2401 ccaatctcgg gctgcaccgc gacgatcgga ttaccgaagc agcagttctt gacctcgttg
     2461 tgcgtcatgc cgtcgccctc ggtctggcca acgacgtagc cggagcccgc gccgccggcg
     2521 agcgcgatca tcacgcgtgc gcgcttggga ccgacggtga cgttgctgga gacgctgccg
     2581 ccgccaccac cgccaccgcc gccgccgccg ccgatcggat cctcgttgtc acccgagcct
     2641 cccgcggcga cgccgctgat ctcgatgatg ttcggcgagc ccgacgagcc cttcgacgcc
     2701 acgggcttgg gaccgtccgc gtacgcagcg acgtagtagt gcacgagcga gcccttcatc
     2761 gctgccgccg ggatcgagcc ggtgtacttg cagtcgccgt cctttttcat tttgacttcg
     2821 gtgaagtccg tcgcgccttc cgcgcgatag aggatcgaga ccttcgtcgt gccggcaacg
     2881 tcagcgccga gatacgcctc gaccgcgagc ggagcgccgc ccttcgcagt gtcgatgatc
     2941 gtgtgctgca tgccctgcac cgtcgaacaa tcgacgccgc tcgtatcaac tggaggaggc
     3001 gtggcctcga cgggctccga cgcaccgccg ccaccgctcg actccttgcg cgcctgctct
     3061 aggagcttcg acatctcgtt cgacttgtag gcagcgtcga tctggatctt cggatcgatc
     3121 tgcaccgccg acaggaacga gagcttcgcg ccgtccgcat cgttgttgac gaactgcacg
     3181 atgccgagat cgaggtgcgc tttcgcggtg cccggatcct tgtcgagctt gttcttcttg
     3241 gcgatctgga gcgcctgatt gagcagcttc ttcgctgcgt cgtaatccat caggtcgtag
     3301 ttctccatcg cctccttgat cttggtttgg atatcgcttt tcggactggc gtccgcgatt
     3361 ccgaaaccga acaagatcgc gatgaccgcg acgagactgc ttcccctcat gtgggtgtca
     3421 taacaagaaa tccggcgggc cagcggcaaa aagacctaac cactgacggg atcgaggcta
     3481 attgcacacc tcgttttcga cgtacggacg cccaaatccc gtcgaggacg gcctgatggc
     3541 gcccgccacc actcgcctgc ggcgtggccc gtacagcgcg attagcgctc ggtaatgacg
     3601 aactcgacgc ggcggttctg cgaccggccg tcggccgtgc ggttgtcggc gatgggtacg
     3661 gtctcgccgt aacccttcgg gaccatgcga tctgcgctca cgccccgctt cgcgagatac
     3721 gccttcacgc tctcggcgcg tttctggctc agcttcatgt tgaaggcgtc gttgccgacc
     3781 gagtcggtgt ggccctgaac ctcgaccttg atggtcgggt tgtcctgcat cgcctgcgcg
     3841 acctcgttga gcagcgggaa cgagaccggc ttgatggtcg ccttgttgaa ctcgaagaag
     3901 atggtctgct tgatctcgat cttcgtcttc gtgacgacga cgttgacgta cttcttcggg
     3961 cagccgtcgg gcgcgacgcc gaactcgagc gggcacttgt cgtccttgtc gaggaacgtg
     4021 tcgttgtcgt tgtcgaggtc gggacaaccg tcgtcgtcct cgaagccatc cttgtcctcg
     4081 ggctcgttcg ggcactgatc gaccttgtcg gcaaggccgt ccttgtcgtt gtcgaggtcg
     4141 gggcagccgt cctcgtcttc gaacttgtcg aggtcctcgg gatccatcgg acacttgtcg
     4201 acgacgtctt tgatgccgtc ggcatcgttg tcgagatccg ggcagccgtc gtcgtcctcg
     4261 aagccgtcct tgtcttcggg atcgtcggga cacttgtcga cgtcgtcttt cagcccgtcg
     4321 cgatcgcgat cgccaaccgg cggcgggccc gacgggttgc acttgtcctt cggcgatttt
     4381 tcgatcgcga gcttcgcgtt cttctcggcg atgtcggcgg tacggcggcc gtcgaagtag
     4441 ttgccctcgt cgagcgcgtg gatcgcgaag tcgttgtacg actcggccat cgcgagctcg
     4501 acgggcgcgc acttcatcgc gccgttgtcg cgcgcggtcg cgatcagacc gaccgtgtcg
     4561 gtcgtcttgg aacgcacctg ttgtcccgca caaccaacga gcacgcagag cgcgaggacg
     4621 agcgacttca tcattctttc gcgggcgcca cgccgccccc agtggctgag gacgaccccg
     4681 gcgccgtact gccgtcgtct ttggcgggcg caagctcttg ggtcggattg accggacgct
     4741 tgctcggatc gctcgcggcg gccgccgatt cctcggctgc cttctcggcg gcttccgacg
     4801 acagccggcc gaagtgattc gcgccctgat agtcggcgtg tcccgcaacc tcacgcgcca
     4861 tgtgcagata cacgacggcg cgcgtccacc agtacgggct gtacttctcg gcatcgagcg
     4921 atcgcgcgcg ctcgaccttg tccgcggcgt cgtacgggac ctgcttgatg tactggatcg
     4981 ggccacagcc tgacagcaag gccagcgcga tcacccaagg tcgcatcggc ctcatcatat
     5041 caccgcgacc gaacccgccg cccgtctcgg cctagctcga tcgcgacgta gatcgcggac
     5101 ccgaacagcg cgaacagcga cacgacgaat gcccagccca gcttgacggc gaacagcgac
     5161 atgccggcac ccgccgcata gcccggatag tcggcggccg tcgacagccg gttgatgccg
     5221 accgcgacga ggacgagcat gaacagcgcg agcgcgccgg ccgcatagac cagcgcgccg
     5281 gcgagcccct tgcggatcag gaacgccgag atgtaccagc cgccgatcac gagcgcggcg
     5341 tggccgacca tcagcggcag cacgaagatc gtggccagct tcgcggggtc gacccagtac
     5401 atccatgccc acgcgggatg gacggcgtag aagtagagcg cgatcggagc gacgaccgca
     5461 gctgcatgca tcgcgacgag cggaaacgcc ggcgacgcaa acggaccatc ggctttaatg
     5521 cggtcccgcg cgatcagcgc gaagccgatc ccgaacagca aattgatcag ggcgacaacg
     5581 ggaatggcgc aggccctccg cactgaagct actacgtcct gcggtgcttc ggcgcgctgc
     5641 gttaccgaga tcggctagga actgcttcga gcggcggcgt tcggctgccg gtatcggggt
     5701 ccggttgctc cgcgttgccg agcagcttgc tggccgcggt gaactcgacc ttcggggtct
     5761 tcgcatccgc gccctcggga gccggcgtgg gaacgtcgat ttcgccggcc acgaacgcgt
     5821 tctccgatga aatcgtgccg cccggcggca ccacggtgac gccctccacc ggcgtgccgt
     5881 cgtcgttgac cgcgccggtc tcctggttat cgtcgtcggg cgcgcgcacg gcttgcgcga
     5941 cgttggtgac gacccaatcg tcctgaccgg cgggtggcga ggtcagcgcg gattgcgtcc
     6001 acgcctcgct gtcggcccgc tgcttcggtt tgggaatcgg cggcatcggc ggtggcgcga
     6061 ccggatggcg ctccatcgtc tgatcggcgg gcggcggcgg cgcgacgacc gagttcgagc
     6121 tggtgtggcc gcgcgcgagc tcgtcgagcg actcgggatc ctcgatctga ttgagcgcgg
     6181 tgtcgaccgg cggcgcctgc ggcgcttctt cgaacacggt cgcttcgaag tcgtcatcgg
     6241 cgagcatctc gatcttcggc gcgtcgtcgt cctccgcttc ctcggagaac tgatcggcaa
     6301 accgctcggc gatgatcgtg tatgccttgc gcagcgcgcg gcgtgcagag ctcgccgtga
     6361 agtcgtggcg cacgcgctcg taggccgcgt tggcgaggcg atcgcgcagc tgcggctcgc
     6421 cgagcaggcg aagcaccttg cgcgcgagat ccaccggatc gccgggctcg aacagcagcg
     6481 cctcgcggcc gttctcgacg acgagctgca cggtgttgcg ccgcggcgcc acgatcgcgc
     6541 gccggcacgc catgtactcg agcatcttcg tcggaaagac gaccgccgga ttcgccgtga
     6601 ggtcggcggc cgcgggaacg acgcacaccg tcgcggtcgc gatcagcgcc ggcaattgat
     6661 cgtggtcgac ggcaccgacc atctcgacct tctcggtgag cccgagctcg cgaatgccgt
     6721 cgcgcagcgc ggtttcgaac ttcggtgcga tgaccgggcc cgcgatgacg agccgggcat
     6781 cgacctcccg tacgatcgcg gccatcgcgc ggatcaacac gcgcacgccg cgaccgggat
     6841 cgatcgagcc ggtgtacagg atgcgtggcg gtccactctt cgtcgcctcg tcccagtcga
     6901 agcgatcgac atcgacgccg ggcggcgaca gcagaacgcg ctctggcttg ccgcgcccct
     6961 gcagcgcttt gaccgcggcg ggagtcggcg cgagcacgag gtcgctcgca agaatgcagg
     7021 cctcttcatc gcggccgtac tgcgcatcga gctctggatc gagcgtggtc tcgccgagcg
     7081 gcgagcgcgt catgtcgtag acgaccgcgt agccgagtcg cgagcgcgct tcgagcaccg
     7141 gaatgcagct ccagctgtcg cggcaatgca cgacgtcgta gtcggcgccc tcgagctggc
     7201 gcttgagcgc gcgctgatac gattgaatct gagcgcgcag atccgcgtcg tgcgtcggca
     7261 cgcgcaacac ccgtacggaa ccttgccgtt cgacgtaggc ctgctcaccc tcgcggagca
     7321 cgagcaaatc gaccgtgtga aacggcgtca acgagcgaat ca
//
LOCUS       DASUFX010000781         7145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1514124, whole genome shotgun sequence.
ACCESSION   DASUFX010000781 DASUFX010000000
VERSION     DASUFX010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514124"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFV99_31575"
     CDS             complement(<1..1724)
                     /locus_tag="VFV99_31575"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5063977.1"
                     /translation="MRWLALACFVVACAHQGAGPSGDDDDGQEPWQPGLGAHWSPAGD
                     EVSFRIASTRATRIELWIYAQPTQSAEVLHAELARETDTTFGVRVPASALPPTIYYGY
                     RVWGPNWPYEPAWQPGTDIGWITDVDSDGNRMNPNKLVIDPYARELSHDPNTPAQLDG
                     APYAVGMRRTIDSAPVAPKAIVLHDEAVTSDKPARPLRDDVIYEVHVRGFTKAAPIEC
                     AGTYAGAAERAAYLRDLGVTAIEFLPLQESNNDRNDVDPNSASGDNYWGYSTVAYFAP
                     DRRYACDQSAGGPTREFRDMVHAFHAEGIKVLVDVVYNHTAEGGGGSLLSLRGIDNAG
                     YYQLDRSGTGFTNSNGVGADLAMDKPLAKQLMLDSLRYFYDELGVDGFRFDLAPVLGN
                     VCGPGCFKFGSGVHEEIAAAFPDAALIAEPWGVVAGSYQVGHFPAGWSEWNDKFRDTI
                     RGDQNKAQTTPGWIADRVHGSTELFRDDGRMPSASINYLVSHDGFTLRDLYACNGPNN
                     TQAWPYGPSDGGSANNLSSDHGGDAVAQRQAARTGMALLMVSAGVPMLVGGDEMLHGV
                     RCNNNYYNL"
     gene            1848..3074
                     /locus_tag="VFV99_31580"
     CDS             1848..3074
                     /locus_tag="VFV99_31580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063978.1"
                     /translation="MRPGIVWMGLLAACYTGSPAPVGQTPFPPPSAPVRKIAAPTNPN
                     RVRIDLADEASLVAAGFEHNAGGTSRFANGRLEITTNSYEEWMQDEGFVQQAANGPWA
                     VEARFAITTPCARGGTGVWIHDGMYFVRAAITDHELVLDRQRVEIGPTSQLRTYRFEL
                     ADKQVTVLVDGKQVLQKPASEIAASVTLMFGVLGDGCTANSSTWDYIAYETTPGPGAF
                     WPPRWDWHAGTKAQPLIDALHKQLPSLAIPQLDDRDVPCLAIFTLDGALRDVLPLAYD
                     AQSTPNVATEFRRLPVMTNTHALGDADRTIRTWTEPQGGPYPCDPAPGTRCPPPVQPK
                     PAAPVPAAAAVLRTAVWNASRWSLHPDYAERSTAMAAKVYADAIAAKLSGATEAVKRL
                     QAKLSSLANHPKKCGL"
     gene            3114..3884
                     /locus_tag="VFV99_31585"
     CDS             3114..3884
                     /locus_tag="VFV99_31585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063979.1"
                     /translation="MSSLEYVDVATARAARGTRIITSALVASPWSEAAKGMFVVANLP
                     ALVVGRPRDAADITQWTGVDNVPVVLHDDEPARTNWAAIVGLASRLAGPDVLVPSDPE
                     QRSALMGLVELVAGEQGLGWNARLSMIHASFESEGKRGFSLPVAGYLAKRYGHTRSLD
                     TDALRGRVANQLRILDEKLTGSYFAGTRPGALDVYAATFLTPLTVIDDAVCPQMAPPI
                     RTAFAAAKDLLGDLVPARLWAHRTMMFERHLALPIRLA"
     gene            complement(3917..4750)
                     /locus_tag="VFV99_31590"
     CDS             complement(3917..4750)
                     /locus_tag="VFV99_31590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063980.1"
                     /translation="MLASLPGDAKLIFAVDVTKLRHSPIAQRGWSLLRELPMVDELIG
                     TMCGALDDVKYALVGLEDADGHKSWGWLHGVNRDAKLTCTKAVEQQKLKQTKSFSRGD
                     YSLETSTTSTTEILWLDATTAFYFQHAPDDKLIGEPELRRVLAAGGGFTQGDLATLLD
                     HVDFESGLVMVGDGAMTKQPGIGGLAFSIEADAGLRAQAYVQLENEDRASGIQSQMRA
                     VLAQLVKNRMLESAEARAEGKGLAASIEMTGAQFDWWIELALAKAKDPNAPIPPPPSA
                     N"
     gene            5076..5597
                     /locus_tag="VFV99_31595"
     CDS             5076..5597
                     /locus_tag="VFV99_31595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5063981.1"
                     /translation="MALPGLSHVDHVALTVPDLEQAVKFYCEVIGGKELYRLGPFDAE
                     QIPRMPDGRDWTDAHINVAGARLMIAMLQLGPNVMLELFQYEKPADARTTPPRNCDLG
                     GHHIALKVENIDAAIAYLKERGLTVLAGPTLLAEGPAAGLRFNYFLDPWGNHLELIEY
                     KTLPWMQAAGLKP"
     gene            complement(5598..>7145)
                     /locus_tag="VFV99_31600"
     CDS             complement(5598..>7145)
                     /locus_tag="VFV99_31600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5063982.1"
                     /translation="DLSEPDNIAGNSAFRRLVVLDLVDDLTPSATPVIVAEAKPIPTP
                     RAPPPRPRPRPGGEQEQVQQQAQVIEVDPNVYVQNMSSNQVYWQQLENNNQIGNAMPG
                     GANVANAPPPQQVRTPPQPQTGTPTTQDASPAQAQIKPPVQTPPVQRPPAQRPPQPAE
                     PPPPITITISPLEWRTLTFGGKPTLIAVRNVQTPDGTFSQGFVVDRSALTSALAAKAG
                     EMVAELHDGDRASGVDIAPGWQLTVTPNPRTLARAAGSAADIARSFVIRFVAVGSIAF
                     IAAALILLLVMRAEKLARERSQFAAAAAHELRTPLAGLQLYGDMLADGLGDQSKVRDY
                     ARRMSEEASRLGRVVSNVLGFSQLERGNLAVDSQVGPLGEALRELAERAEPALDRAGA
                     ALELDVAPDLRARFDRDALARIVGNLLDNAEKYARGADDRTIRLAAADRGNLVEVIVQ
                     DHGPGVADRAKLFRAFSRGVAADGPAGLGLGLALSQSLAEAMGGKLDYRPGDGAGATF
                     VLELPRA"
BASE COUNT         1138 a         2360 c         2444 g         1203 t
ORIGIN      
        1 aggttgtaat agttgttgtt gcagcgcacg ccgtgcagca tctcgtcgcc gccgacgagc
       61 atcggcacgc cagcggacac catcagcagc gccatcccgg tgcgcgctgc ttgccgctgc
      121 gccactgcgt cgccaccgtg atccgacgac aggttgttcg cgctgccgcc atcagacggg
      181 ccgtagggcc acgcctgcgt gttgttgggt ccgttgcacg cgtagaggtc gcgcagcgtg
      241 aagccgtcgt gcgagaccag gtaattgatc gacgcgctcg gcatgcggcc gtcgtcgcga
      301 aacagctcgg tgctgccatg cacgcgatcg gcgatccagc cgggcgtcgt ctgcgccttg
      361 ttctgatcgc cgcgaatcgt gtcgcggaac ttgtcgttcc attccgacca accggcggga
      421 aagtgaccga cctgatagct gccggcgacc acgccccacg gctcggcgat cagcgccgca
      481 tcggggaacg cggccgcgat ctcctcgtgc acgccgctgc cgaacttgaa gcagccgggg
      541 ccgcacacat tgccgagcac gggggcgagg tcgaagcgaa agccgtcgac accgagctcg
      601 tcgtagaagt agcgcaacga gtcgagcatc aactgcttcg cgagcggctt gtccatggcg
      661 aggtccgcgc cgacgccgtt gctgttcgtg aacccggtgc cactccgatc gagctggtag
      721 tagccggcgt tgtcgatgcc gcgcagtgag agcagcgagc cgccgccgcc ctcggcggtg
      781 tggttgtaga cgacgtcgac gagcaccttg atgccctcgg catgaaacgc gtggaccatg
      841 tcgcgaaact cgcgcgtcgg gccgcccgct gactgatcgc atgcgtaccg gcgatcgggc
      901 gcgaagtacg ccaccgtcga gtagccccag tagttgtcac cgctcgcgct gttcggatcg
      961 acgtcgttgc gatcgttgtt cgactcttgc agcggcagaa actcgatggc cgtcacgccg
     1021 aggtcgcgca gatatgccgc gcgctctgcc gcgccggcgt aggtaccagc gcactcgatc
     1081 ggcgctgcct tcgtaaagcc gcgcacgtgt acctcgtaga tcacgtcgtc gcgcagcggc
     1141 cgcgcgggct tgtcgctagt caccgcttcg tcgtgcagca cgatcgcctt tggcgcgacc
     1201 ggtgccgaat cgatcgtgcg gcgcatgccg acggcgtacg gtgcaccgtc gagctgcgcc
     1261 ggcgtgttcg gatcgtgtga cagctcgcgc gcgtacggat cgatcacgag cttgttcgga
     1321 ttcatccgat tgccgtcact gtcgacatcg gtgatccagc cgatgtccgt ccccggctgc
     1381 catgcaggct cgtacggcca gttcggtccc cacacgcgat agccgtaata gatcgtcggc
     1441 ggcagcgcgc tcgctggcac gcgcacgccg aacgtggtgt cggtctcgcg cgccagctcg
     1501 gcgtgcagca cctcggcgct ctgtgtcggc tgcgcgtaga tccacagctc gatgcgcgtc
     1561 gcgcgcgtcg acgcaatgcg aaacgacacc tcgtcgccgg ctgggctcca gtgcgcgccg
     1621 agtccgggct gccacggctc ttgcccgtcg tcatcatcgc cggacggccc ggcgccttga
     1681 tgcgcgcacg caacgacgaa gcaagctagc gcgagccacc tcatcgcgtg ctgcacgatg
     1741 caacgcacag gccgcgccag acacgcgatt ctgcgtgggt gcgccgctcg tcacgttgcc
     1801 gatcgcaacg cgtcgatcgc aacgtggtcg catccgtata atgaggcatg cgccccggaa
     1861 tagtgtggat gggactgctt gcggcttgct acacggggtc gccggcgccg gtcggccaaa
     1921 cgccgtttcc gccgccgagc gcaccggtaa ggaagattgc cgcgccaaca aatcccaatc
     1981 gcgtgcggat cgacctcgct gacgaggcgt cgctcgtcgc cgcagggttc gagcacaacg
     2041 ccggcggcac gagtcgcttc gcgaacgggc ggctcgaaat cacgacgaac agctacgagg
     2101 aatggatgca ggacgaaggc ttcgtccagc aagcggcgaa tggcccgtgg gccgtcgaag
     2161 cgcgcttcgc gataacgacg ccgtgcgcgc gcggcggcac cggcgtgtgg atccacgacg
     2221 gcatgtactt cgtgagggcc gcgatcaccg accacgagct cgtgctcgat cgccagcgcg
     2281 tcgagatcgg tccgacgagc cagctccgca cgtatcgctt cgagctcgcc gacaagcagg
     2341 tcacagttct cgtcgacggc aagcaagtgc tccagaagcc ggcgtccgag atcgccgcgt
     2401 cggtgacgct gatgttcggc gtgctcggcg acggctgcac ggcgaactcg tcgacgtggg
     2461 actacatcgc gtacgagacg acgcccgggc caggtgcatt ctggccaccg cgttgggact
     2521 ggcacgcggg cacgaaggcg cagccgctga tcgatgcgct gcacaagcag ctgccgtcgc
     2581 tcgcgatccc gcagctcgat gatcgcgacg tgccgtgtct cgcgatcttc acgctcgatg
     2641 gcgcactacg ggatgtgctg ccgctcgcct acgacgcgca gagcacaccg aacgtggcga
     2701 ccgagttccg ccgcctgccg gtcatgacaa acacgcacgc gctcggcgat gcggaccgca
     2761 cgatccggac ctggaccgag ccgcaaggcg gtccgtaccc gtgcgatccc gcgccgggga
     2821 ctcggtgccc gccgcccgtc cagccgaagc ccgctgcgcc ggtgccggcg gctgcagcgg
     2881 tgctgcgcac tgcggtgtgg aatgcttcgc gatggtcgct gcatcctgac tatgcggagc
     2941 gctcgacggc gatggccgcg aaggtctacg cagacgccat cgcagcgaag ctgtcgggtg
     3001 cgaccgaggc ggtcaaacgc ctgcaggcaa agctgtcgtc actcgcgaat cacccgaaga
     3061 agtgcggcct gtaatttctg gttgtctctg cgtgaggcac aatagcgccg ctcatgtcgt
     3121 ccctcgaata cgtcgacgtc gcgaccgcac gtgcggctcg cggaactcgc atcatcactt
     3181 ccgcgctcgt cgcaagtccg tggtcggagg cggcgaaggg catgttcgtc gtcgcgaatc
     3241 ttcctgcgct cgtcgttggc aggccgcgtg acgccgccga catcacgcaa tggaccggcg
     3301 tcgacaacgt gccggtcgtc ctccacgacg acgagccggc acgcacgaac tgggcggcga
     3361 tcgtcggcct cgcgtcgcgc ctggccggcc ccgatgtcct cgtgccgagc gacccggagc
     3421 agcgatccgc cctgatgggc ctcgtcgagc tcgtcgcagg tgagcaagga ctcggttgga
     3481 atgctcgact ctcgatgatc cacgcgtcgt tcgagtcgga gggcaagcga gggttctcgc
     3541 tgccggtcgc cggctatctc gcgaagcgct acggccacac gaggtcgctc gataccgacg
     3601 cgctgcgcgg acgcgtcgcg aaccagctgc gcatcctcga cgagaagttg accggtagct
     3661 acttcgccgg aacgcgcccc ggcgcgctcg acgtctatgc ggcaacgttt ctcaccccgc
     3721 tgaccgtgat cgacgacgca gtctgtccgc aaatggcgcc gccgatccgc acggcgttcg
     3781 cagctgcgaa ggatcttctc ggcgacctcg tgccggcgcg actgtgggcg catcggacga
     3841 tgatgttcga gcgtcatctc gcgctgccga ttcggctcgc ctgacggtcg ccgcccgctc
     3901 gttcgggttc gttccgctag ttcgcgctcg gcggtggtgg gatcggcgcg ttgggatctt
     3961 tcgccttcgc cagcgcgagc tcgatccacc agtcgaactg cgcgccggtc atctcgatcg
     4021 aggcagccag gcccttccct tcggcgcgag cctcggcgct ctccagcatg cggttcttga
     4081 cgagctgtgc gagcacggct cgcatttggg attggatccc gctcgcccga tcctcgttct
     4141 cgagctggac atatgcctgc gcacgtagtc cggcatcggc ttcgatcgag aacgcgagac
     4201 caccgatgcc gggctgcttc gtcatcgcgc cgtcccctac catcacgagc ccggattcga
     4261 agtcgacgtg atcgagcagc gtagcaaggt cgccttgcgt gaaaccgcca ccggcggcca
     4321 gaacgcggcg cagctctggc tcgccgatca gcttgtcgtc gggcgcatgc tggaagtaga
     4381 acgccgttgt cgcgtcgagc cagaggatct cggtcgtgct cgtcgtcgag gtctcgagcg
     4441 agtaatcgcc tcgcgagaag gacttggtct gtttcagctt ctgctgctcg accgctttcg
     4501 tgcacgtgag cttcgcgtcg cggttgacgc cgtgcaacca gccccatgat ttgtgaccgt
     4561 cggcatcttc gaggccgaca agtgcgtact tgacgtcgtc gagcgcgccg cacatcgtgc
     4621 cgatcagctc gtcgaccatc ggcagctcgc gcaacaacga ccagccgcgt tgcgcgatcg
     4681 ggctatgacg tagctttgtg acgtcgacgg caaagatcag cttcgcatca cccggcaacg
     4741 acgcgagcat gtcagcgcga gtgatccgac gcggcgggtg caccgttact gatggcgagc
     4801 tcggaggagg cgacgagact ggcggcgggc tgccaccgca ggcggcgagt acgcaagcaa
     4861 ggacgacgct gcgcatgccg atcgattatc gctgtttcga cggctcgcga gcgccggatc
     4921 gacgtcaccg atgtaggcaa tcgggcaccg gtgccaccgt ttgcggcacc gtgctcgagc
     4981 gcgcgggtcg acgagcagcc gcattatgcg gcaggggtac gtgcagacgc tcgagcagcc
     5041 gcgtgctctg ctcggcgcaa ccaaggggga agactatggc gttgccagga ttgagccacg
     5101 tcgatcacgt ggcattgacg gttcctgacc tcgagcaggc ggtgaagttt tactgtgagg
     5161 tgatcggcgg caaggagctc tatcggctcg ggccgttcga cgcggaacag ataccgcgca
     5221 tgcccgacgg gcgcgactgg accgacgcgc acatcaacgt cgcgggcgca cgcctgatga
     5281 tcgcgatgct gcagctcgga ccgaacgtga tgctcgagtt gtttcagtac gagaagcccg
     5341 ccgatgcgcg cacgacgccg ccgcgcaatt gcgatctcgg cggtcatcac atcgcactca
     5401 aagtcgagaa catcgacgct gcgatcgcgt atctgaaaga gcgcggcctg acggtgctcg
     5461 ctggcccgac gctgctcgcc gagggtccgg cggccgggct gcgcttcaac tacttcctcg
     5521 atccatgggg caaccacctc gagctgatcg agtacaagac gctgccgtgg atgcaggccg
     5581 caggcctcaa gccctgatca ggcgcgcggt agctcgagca cgaacgtcgc gccggcgccg
     5641 tcgcccggcc ggtagtcgag cttgccgccc atcgcttcgg cgagcgattg cgagagcgcg
     5701 aggccgaggc caaggccggc tgggccgtcc gcggcaacgc cgcgcgagaa tgctcggaac
     5761 agcttcgcgc gatcagcgac gccggggccg tggtcctgca cgatcacctc gacgaggttg
     5821 ccgcgatcgg cagccgcaag acgaatcgtg cggtcgtcgg caccgcgcgc gtacttctcg
     5881 gcgttgtcga gcaggttgcc cacgatacgg gcgagcgcgt cgcgatcgaa ccgcgcgcga
     5941 aggtcgggcg cgacgtcgag ctcgagagcc gcgccggcac gatcgagcgc gggctccgca
     6001 cgttccgcga gctcgcggag tgcttcaccg agtggaccga cttgcgaatc gaccgcgagg
     6061 ttgccgcgct cgagctgcga gaagccgagc acgttggaga cgacgcggcc gaggcgagac
     6121 gcttcttcgc tcatgcggcg cgcgtagtcg cgaaccttcg attgatcgcc gaggccatcg
     6181 gcgagcatgt cgccgtagag ctgaaggccg gcgagcggcg tgcgcagctc gtgggcagcg
     6241 gcggcagcga attgactgcg ctcgcgcgcg agcttttcgg cacgcatcac gagcaacaag
     6301 atgagggccg ccgcgatgaa ggcgatcgaa ccgacggcga cgaaccggat gacgaacgag
     6361 cgcgcgatgt ccgcggccga gccagccgcg cgggcgagcg tgcgcgggtt cggcgtgacg
     6421 gtgagctgcc agcccggtgc gatgtcgacg ccggatgcgc gatccccgtc gtgcagctcg
     6481 gcgaccattt caccggcctt ggcagcgagc gcgctggtga gcgcggagcg atcgacgacg
     6541 aagccttgtg aaaacgtgcc gtcgggcgtc tgtacgttgc gcacggcgat cagcgtcggt
     6601 ttgccgccga acgtcagcgt gcgccattcg agcggcgaga tcgtgatcgt gatcggcggc
     6661 ggtggctcgg cgggctgcgg cggacgctgg gccggcggac gttgcaccgg cggcgtttgc
     6721 accggcggct tgatctgagc ttgcgccggg gacgcatctt gggtcgtcgg cgtgccggtc
     6781 tgcggttgcg gcggcgtgcg gacctgttgc ggcggcgggg cgttcgcgac gttggcgccg
     6841 cccggcatcg cgttgccgat ctggttgttg ttctcgagct gctgccagta gacctggttc
     6901 gagctcatgt tctggacgta gacgttcgga tcgacctcga tcacctgtgc ctgttgctgc
     6961 acctgctcct gctcgccgcc tgggcgcggg cgaggccgcg gcggcggggc gcgcggcgtc
     7021 ggaatcggct tggcctcggc cacgatgacg ggcgtcgccg atggcgtcag atcgtcgacg
     7081 agatcgagca cgaccaggcg acggaacgcg ctgttgcccg cgatgttgtc gggctcgctg
     7141 agatc
//
LOCUS       DASUFX010000782         6383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1098602, whole genome shotgun sequence.
ACCESSION   DASUFX010000782 DASUFX010000000
VERSION     DASUFX010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098602"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFV99_31605"
     CDS             complement(<1..655)
                     /locus_tag="VFV99_31605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063983.1"
                     /translation="MRPLVILLAVIGLGGVALADVPWAQGVAKDKQTQANALFAEGNE
                     LFAQQAHAPALEKYKAAVALWDHPLIEFNMAVTLVRLDRILEAADSLDKALRFGEAPF
                     PSAEQYQQALDYKKLVAGRVGTLQLSCDAPATVLLDGQAWLTCPGNREARVLAGEHVI
                     VAEREGYYPLTRHVFVSGGTTAREALSLVRLDRVKLEYPTPRWIPWAIAGGGLAVTLG
                     "
     gene            complement(652..3402)
                     /locus_tag="VFV99_31610"
     CDS             complement(652..3402)
                     /locus_tag="VFV99_31610"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5063984.1"
                     /translation="MRKRRESAHPLDFSSKTTIGRFELIREIARGGMGQVFLGRDTKL
                     GRKVAIKFLLRDDTQLVQRFLVEARATARCTHENIVTIFEVGEHERLPYMVLEYLEGK
                     TLTEVMETKPSMRQFCELMVPVARALERAHEHGIVHRDLKPSNIFVTVRGQVKVLDFG
                     VARLMGRAENHIKRATAGGDVTHVDDEPAGASLTETNDDVRDKEHVLVGTLPYMSPEQ
                     WGAGEVDHQSDIWAVGVMFWRALAATHPAGTMVPEKLRARVCDLDTPLPSIGPRVPSL
                     PKELVAIVDRCLAKHKLYRYQNATELLADLQAFLVPRPERTGRTTSEVCPYRGLSAFG
                     EDDAKYFFGRDNEIRTALGSLDVWPLLAVIGPSGVGKSSFVHAGLIPALRSRGGEWQV
                     HVLRPGRLPLQRLALVLEEGPGQAEHLLKQLVEAPGLFGVMLRDAAVRRGTNILVVVD
                     QLEELFTLAEGDDVRRLFLAALLAAADDPLSPVRVVLSMRADFLDRLAGHKHFLAELS
                     RGMFFLTAPDQDNLRETLERPAELAGYSFESSSVVEDIMQTATSRGALPLLSFAATRL
                     WEARDRQRKLLTVAAYQQMGGIAGAFARHADQVAAAVPPQHHLLLRAIMTRLVTPEGT
                     RAVVDHGELLSLATDQKAVELVLDHLVRGRLIQMQTDPDQGATVEIVHEMLISEWPTL
                     KRWLEDGNAMRAFKHELRQAAKQWQTRGKPGDLVWRGAIAQEALANVRRQVLDLSASE
                     QEFVTAIIRRRLRGMLAILAAVVLLVGGVGFAFIKITQANGQARANLDTAEKALGREK
                     AAKLELEEQLHKLEAAQRARESAEKKSETIQTQLTETQQLSRQELEAANQLLQKQVRE
                     AQDAKRKAEAEEAKARRANEEALAAKAQTEKLLDEKRREVEQLKKRKKDIIDVDLTGG
                     TK"
     gene            complement(3489..4082)
                     /gene="miaE"
                     /locus_tag="VFV99_31615"
     CDS             complement(3489..4082)
                     /gene="miaE"
                     /locus_tag="VFV99_31615"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HEX5063985.1"
                     /translation="MEEVPQLGDALPLVPSDPQWISVAMSDLDAVHADHLHCERKAAQ
                     SALSLIRSYPERADIVTAMARLAHDETRHVIQVSQLMARREQPAAYDFGDDYAAALRD
                     HIRKQEPARLLDRLLVFAVIEGRSAERLDLLAKALPDPKDAQLYASLATAEVRHRDTF
                     LALARDTSPETWRVRAAELATLEAEILANRPVIARIH"
     gene            4110..6020
                     /locus_tag="VFV99_31620"
     CDS             4110..6020
                     /locus_tag="VFV99_31620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5063986.1"
                     /translation="MKKETKKFTEECLIGLKRAQQLRPQIVATTPRTIDSTLVSYNEL
                     LTAASATNAMAGLMSEVHPDEAIRDAARECEQEVSRFYSDLALDRDMYDALAAVDVSA
                     ADADTKRFVEHTLRDYRRAGVDKPIEVRERLKAIDEELTKLGQQFSKNISEDVRAVEV
                     KDASRLGGLPADFVASHAPDANGTIRITTDYPDYNPFMTYATDDALRRELYIKFRSRG
                     DQANEAVLRDVLKLRAEKASLLGYKDWADYITADKMIQSGQRASDFIEKVWKLAAPRA
                     EQDYAELLRTQQAIDPSAKEVADWQKVFLENIVKKERYEVDASEVRQYFPYDRVLAGL
                     LEITSEIFELTYAPVKDAQPWHASVLVYDVMRGNTKLGRIYLDMHPREGKYKHAAQFP
                     LKDGVRGVQLPEGVLVCNFPEPASGNALMEHDDVVTMFHEFGHLMHHVLGGHQKWITQ
                     SGVATEWDFVEAPSQMFEEWAWSHDTLARFARHHKTGEVIPEALVEKMRRADKFGLGT
                     ATVQQIFYAAISLGFHRADPDKLDQLGEVQRLQKQYTPFRYVPGTRFHASFGHLVGYS
                     AMYYTYQWSLVIAKDLLTPFQHKGLMSKDVTLAYRDKVLAPGGSRDAAELVRNFLGRD
                     YDFAAFERYLVS"
     gene            6107..>6383
                     /locus_tag="VFV99_31625"
     CDS             6107..>6383
                     /locus_tag="VFV99_31625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063987.1"
                     /translation="MKKLTLAFMVAMAFVSFGCKKKGGGGAAEAMAKMTEFKDEMCKC
                     KDAKCAQDVSDKMTKWSQEQSKNQKEPPKMSEEDTKKAAAIGEEMGKC"
BASE COUNT         1124 a         2082 c         2072 g         1105 t
ORIGIN      
        1 cgccgagcgt gaccgcgaga ccaccgcccg cgatcgccca cgggatccag cgtggcgtcg
       61 gatactcgag cttcacgcga tcgagccgca cgagcgacaa cgcctcgcgc gccgtggtgc
      121 cgccggacac gaacacgtga cgcgtcagcg ggtagtagcc ttcgcgctcg gcgacgatca
      181 cgtgctcgcc ggcgagcacg cgtgcttcac gattgccggg gcaggtgagc cacgcctgcc
      241 cgtcgagcag gaccgttgcc ggtgcgtcgc acgacagctg gagcgtgccg acgcggcccg
      301 caaccagctt cttgtagtcg agcgcctgct ggtactgctc ggccgatgga aacggcgcct
      361 cgccgaagcg cagcgccttg tcgagtgaat cggccgcctc gaggatgcga tcgaggcgca
      421 cgagcgtcac cgccatgttg aactcgatca gcgggtggtc ccacagcgcg accgctgcct
      481 tgtacttctc gagtgcgggc gcgtgcgctt gctgcgcgaa cagctcgttg ccttctgcga
      541 acagcgcgtt cgcctgcgtt tgcttgtcct tcgcgacgcc ctgcgcccac ggcacgtcgg
      601 cgagcgcgac gccaccgagc ccgatcacgg caagcaggat cacgagcggc ctcacttggt
      661 gcctcccgtc aagtcgacgt cgatgatgtc cttcttgcgc ttcttgagct gctcgacctc
      721 gcggcgcttc tcgtcgagga gcttttcggt ttgcgccttc gcagcgagcg cctcttcgtt
      781 ggcgcgtcgc gccttcgctt cttcggcttc cgccttgcgc ttcgcgtctt gcgcttcgcg
      841 cacctgcttc tgcaggagct ggtttgcagc ctcgagctcc tgacgcgaca gctgctgcgt
      901 ctcggttagc tgggtctgga tcgtctcgct cttcttctcg gcggactctc gcgcgcgctg
      961 agcagcctcg agcttgtgaa gctgctcctc gagctcgagc ttggccgctt tctctctacc
     1021 gagcgccttc tcggcggtgt cgaggttggc gcgtgcctgg ccattcgcct gcgtgatctt
     1081 gatgaacgcg aagccgacgc caccgacgag cagcacgacc gccgcgagaa tcgccagcat
     1141 gccgcgcagg cgccgccgga tgattgccgt gacgaactcc tgctcgctcg ccgacagatc
     1201 cagcacctga cgccggacgt tcgcgagtgc ttcctgcgcg atcgcgccac gccacaccag
     1261 atcgcccggc ttcccgcgtg tctgccactg cttcgcggcc tgacgcagct cgtgcttgaa
     1321 cgcacgcatc gcgttcccgt cttcgagcca gcgcttcagc gtcggccact cggagatcag
     1381 catctcgtgc acgatctcga cggtcgcgcc ctgatccgga tcggtttgca tctggatcaa
     1441 tcgcccgcgc accaagtggt cgagaacgag ctcgaccgcc ttctgatcgg tcgcgagcga
     1501 gagtagctcg ccatgatcga cgacggcgcg cgtaccctcg ggcgtcacga gccgcgtcat
     1561 gatcgcgcgc aatagcagat gatgctgcgg cggcacggcg gcggcgacct gatcggcgtg
     1621 acgcgcgaat gcgccagcga tgccgcccat ctgttgatag gcggcaaccg taagcagctt
     1681 gcgctggcga tcacgcgctt cccagagccg cgtcgctgcg aacgacagca gcggcagtgc
     1741 accgcggctc gtcgcggtct gcatgatgtc ttcgacgacg ctcgacgact cgaacgagta
     1801 gccggcaagc tccgccggcc gctcgagcgt ctcgcgcagg ttgtcctgat ccggcgcggt
     1861 gaggaagaac atgccgcgcg atagctcggc gaggaagtgc ttgtgcccgg cgagccgatc
     1921 gagaaagtcg gcgcgcatcg acagcacgac gcgtaccggc gacagcggat cgtcggcggc
     1981 ggcgagcagc gcggcgagaa acagccggcg cacgtcgtcg ccttcggcga gcgtgaacag
     2041 ctcctcgagt tgatcgacaa cgacgaggat gttcgtgcca cgccgtaccg ctgcatcgcg
     2101 caacatcacg ccgaacagtc caggcgcttc gacgagctgc ttcaacaggt gctccgcctg
     2161 gccgggccct tcttcgagca cgagcgcgag acgctgcagt ggcaagcgtc ccggtctgag
     2221 cacgtgcact tgccactcgc cgccgcggct acgcaacgcc gggatcagac cggcatgcac
     2281 gaacgacgac ttgccgacgc ccgaggggcc gatcaccgcg agcagcggcc acacgtcgag
     2341 cgagccgagc gcggtacgaa tctcgttgtc gcggccgaag aagtacttcg cgtcgtcttc
     2401 tccgaacgcg gacaacccgc gatacggaca gacctcgctc gtcgtccgcc ctgtgcgctc
     2461 cggccgcggc acgaggaacg cctgcaggtc agcgagcagc tcggtcgcgt tctggtagcg
     2521 atacagtttg tgcttcgcga ggcagcggtc gacgatcgcc acgagctcct tcggcagcga
     2581 cggcacgcgc gggccgatgc tcggcagcgg cgtatcgaga tcgcaaactc gcgcgcgcag
     2641 cttctcgggc accatcgtcc cggccggatg ggtcgcggcg agcgcgcgcc agaacatcac
     2701 gccgaccgcc cagatgtcgg actgatgatc gacctcgccg gcgccccatt gctcgggaga
     2761 catgtagggc agcgtgccga ccagcacgtg ctccttgtcg cgaacgtcgt cgttcgtctc
     2821 ggtgagtgac gcgcctgcag gttcgtcgtc gacgtgggtg acgtcgcccc ccgcagtcgc
     2881 gcgtttgatg tgattctcgg cgcgccccat cagacgcgcg acgccgaagt cgagcacctt
     2941 cacttgcccg cgcacggtca cgaagatgtt gctcggcttc agatcgcgat gaacgatgcc
     3001 gtgctcgtgt gcgcgttcga gtgcgcgcgc gaccggcacc atcagctcgc agaactgccg
     3061 catcgatggc ttggtctcca tcacctcggt caacgtcttg ccctcgaggt actcgagcac
     3121 catgtatggc agccgctcgt gctcgccgac ctcgaagatc gtgacgatgt tctcgtgcgt
     3181 gcaccgcgcc gtcgcacgcg cctcgacgag gaagcgctgc acgagttggg tgtcgtcgcg
     3241 gagcaggaac ttgatcgcga ccttgcggcc gagcttcgtg tcgcggccga ggaacacctg
     3301 acccatgcca cctcgtgcga tctcgcgaat cagctcgaag cggccgatcg tggtcttcga
     3361 cgagaaatcg agcgggtgcg ccgactcgcg gcgcttcctc atcgcttcgg tccgctctct
     3421 atgccccacc tggccgaatc gtaccgcagc ggccggtcga tcgccgttca gggccggtca
     3481 gtcgccgttc agtggatgcg tgcgatgacc ggtcggttgg ccaggatctc ggcttcgagc
     3541 gtggcgagct cggcggcacg cacgcgccac gtctcggggc tcgtatcgcg tgcgagcgcg
     3601 aggaacgtat cgcggtgtcg cacctcggca gtggcaaggc ttgcgtagag ttgcgcatcc
     3661 ttgggatcgg gaagtgcctt cgcgagcagg tcgaggcgct cggcgctgcg gccttcgatg
     3721 accgcgaaca cgagcagtcg atcgagtaag cgagccggct cttgcttgcg aatgtgatcg
     3781 cgcagtgcgg cagcgtagtc gtcgccgaaa tcgtaggcag ccggctgctc gcgacgcgcc
     3841 atcagctgcg agacctggat gacgtgacga gtctcgtcgt gagcaagccg tgccatcgcg
     3901 gtcacgatat cggcgcgctc gggatagctg cggatcagcg acagcgccga ctgcgctgct
     3961 ttgcgctcgc agtgcagatg gtctgcgtgc accgcatcga gatccgacat cgcgacggaa
     4021 atccactgcg ggtcgctcgg tacgagcggc agcgcatcgc ctagctgggg cacctcttcc
     4081 acgacgtgaa tgatgtagca tcgtccgcgg tgaagaagga gacgaagaag ttcacggaag
     4141 agtgcctcat cggcctgaag cgggcgcagc agctgcggcc ccagatcgta gcgaccaccc
     4201 cgcggacgat cgacagcacg ctcgtctcct ataacgagct gctcaccgcc gcgagcgcga
     4261 ccaacgcgat ggcgggcctg atgtcggaag tccatcccga cgaggcgatt cgcgacgccg
     4321 cccgcgagtg cgagcaggaa gtctcacgct tctactcgga cctcgcgctc gatcgcgaca
     4381 tgtacgacgc tctggcggcc gtcgacgtca gcgctgcaga tgccgacacc aagcgcttcg
     4441 tcgagcacac gctgcgcgac taccggcgcg ccggcgtcga caaaccgatc gaagttcgcg
     4501 agcgtctcaa agcgatcgac gaggagctca cgaagctcgg ccagcagttc tcgaagaaca
     4561 tctccgagga cgtccgcgcg gtcgaggtga aggatgcatc gcggctcggc ggcctgccgg
     4621 cagactttgt cgcgtcgcac gcgcccgatg cgaacggcac gattcgcata acgacggact
     4681 accccgacta caacccgttc atgacgtacg cgacggacga tgctctccgc cgcgagctct
     4741 acatcaagtt ccgcagccgc ggcgatcagg cgaacgaggc cgtgctgcgc gacgtgctca
     4801 agcttcgcgc cgagaaggca tcgctgctcg gctacaagga ctgggccgat tacatcacgg
     4861 ccgacaagat gatccagtcg ggccagcggg cgagcgactt catcgagaag gtctggaagc
     4921 tcgccgcgcc gcgcgccgag caggactacg cggagctgtt acgcacgcag caggcgatcg
     4981 atccgagcgc gaaggaagtc gccgactggc agaaggtgtt cctcgagaac atcgtcaaga
     5041 aggagcgcta cgaggtcgat gcgagcgaag tgcgccagta cttcccttac gaccgcgtgc
     5101 tcgccggcct gctcgagatc acgagcgaga tcttcgagct gacgtacgcg ccggtgaagg
     5161 acgcgcagcc gtggcatgcg agcgtgcttg tctacgacgt gatgcgcggc aacacgaagc
     5221 tcggtcgcat ctacctcgac atgcatccgc gcgagggcaa gtacaaacac gcggcgcagt
     5281 tcccgctcaa ggacggcgtg cgcggcgtgc agctgcccga aggcgtgctc gtgtgcaact
     5341 ttcccgagcc agcaagcggc aacgcgctca tggagcacga cgacgtcgtg acgatgttcc
     5401 acgagttcgg tcacctcatg caccacgtgc tcggtggcca tcagaagtgg atcacgcaga
     5461 gcggtgtcgc gaccgagtgg gacttcgtcg aagcgccgtc ccagatgttc gaagagtggg
     5521 cgtggagcca cgacacgctc gcgcggttcg cgcgtcatca caaaaccgga gaggtgattc
     5581 ccgaggcgct cgtcgagaag atgcggcgcg ctgacaagtt cgggctcggc actgcgaccg
     5641 tacagcagat cttctacgcg gcgatctcgc tcggtttcca tcgcgcggat cccgacaagc
     5701 tcgatcagct cggtgaggtg cagcggctac agaagcagta cacgccgttc cgatacgtac
     5761 ccggcacgcg cttccacgcc tcgttcgggc atctcgtcgg ttattcggcg atgtattaca
     5821 cgtaccagtg gtcactcgtg atcgcgaagg acctgctgac gccgttccag cacaagggcc
     5881 tgatgtcgaa ggatgtaacg ctcgcgtacc gcgacaaggt gctcgcaccg ggcggaagtc
     5941 gcgatgctgc tgagctcgta cgcaacttcc tcggtcgcga ttacgacttt gctgcgttcg
     6001 agcgctacct cgtctcgtga tcgttacaag tcgctacaaa acgtcgccat tgattaattg
     6061 attgaacggg cgaccttttt catccttctc attagaggag atatcgatga agaagctgac
     6121 tctcgcgttc atggttgcga tggcattcgt gtcttttggc tgcaagaaga agggcggcgg
     6181 tggcgctgct gaagcaatgg ccaagatgac cgagttcaag gacgagatgt gcaagtgcaa
     6241 ggacgccaag tgcgcccagg acgtctcgga caagatgacg aagtggagcc aggagcagtc
     6301 caagaaccag aaggagccgc cgaagatgtc cgaggaggac acgaagaagg cggctgcgat
     6361 tggtgaggag atgggcaagt gca
//
LOCUS       DASUFX010000783         5662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1794768, whole genome shotgun sequence.
ACCESSION   DASUFX010000783 DASUFX010000000
VERSION     DASUFX010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794768"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFV99_31630"
     CDS             complement(<1..527)
                     /locus_tag="VFV99_31630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063988.1"
                     /translation="MRAIAIALILAVSATTARADEDPIAQAMTLEAQLQYEPALAIIE
                     RTIAQGGADHDRLVTLHQFAGRLAAGLGRATVAEDHFARVLALDPDSKFAAGTSPKIT
                     EPFERARGRTAPLRISSIVEPDAITIVPDADPLGLVAGIAIKLDDGRELREMQTRRVA
                     IPPGKGATEVFALDT"
     gene            complement(628..2283)
                     /locus_tag="VFV99_31635"
     CDS             complement(628..2283)
                     /locus_tag="VFV99_31635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5063989.1"
                     /translation="MSYHPAETAAAPETPTRLGRYELIGQLATGGMAEIHLARLAGEA
                     GFEKTVVVKRLLPELVASKAYVAMFLDEGKLVARLDHPNVCEVHELGRDGAEYFLVMP
                     YLDGVPVHDFIARPRDPDRIAQLRVAAGIVAQACAGLHHAHELKGEDGALLGLVHRDV
                     SPSNLFVTTAGVVKVLDFGIAKVHGGAETEAGTIKGKTQYMAPEQLLGDQLDRRCDVF
                     ALGIVLFELATHSRLFKRSSDYLAARAILEEPIPRADAVDPAVPAALADVIAKALDRA
                     KDKRYADARELAKAVETAMQPHGGVATADQIAAALADWHGAELSAMRTRQAKVIADAR
                     SRATAVTVPGKAAADPSRMLTTPRDVVRKRRLGVIALAVLVVGGTGAVLAISMVREGQ
                     RNSQLAKAPDRVVAETPLPADGDVALAADAAVAVVAPDAAPTGAPAITDFDKLAEKTK
                     ADKRPKPKQDKARGDKQTANPRGDKQSAKVDDTPGRFSVESFPFAKIYIDGTYVDQTP
                     IIGTRLSPGKHTVKAVREDGKIKTFDVTIEPGKLFNKGTLSWN"
     gene            2295..2870
                     /locus_tag="VFV99_31640"
     CDS             2295..2870
                     /locus_tag="VFV99_31640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063990.1"
                     /translation="MPRIALLCLALVGACSFDADYTSGTYSCSDDKCPSGLVCQNQLC
                     VAERRDAAVDVVDTMQGDASDGQMAELSCALPGALAATGGADSGTTVDRTSHVGAQCG
                     GFLMAAGDAVYRITPGAGKQMTISVSTMTTSFMVFAYVTTMCPASVCSPNMVATPGNS
                     LVVTTVAGPQYIVVDSYGGMTGPYMLNVTVN"
     gene            2993..4282
                     /locus_tag="VFV99_31645"
     CDS             2993..4282
                     /locus_tag="VFV99_31645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063991.1"
                     /translation="MTCLLLAGCTLGGTGRPGEGDDTSHGVDDTACGQAMANAPIAPA
                     GYDYHDALPTAAKNTWDGVTLPQPGDSTYPGGRYRSIAADGAGNKHPGCTVAGLMYTP
                     ANIPGYDCAARQFDFPDGVSEDTSKPIVILVHGNSESPTGWVKFVHPDPSSLMFPADT
                     TARDQLAEKLPALGFRTIAVDMRTDKIDDPNSPEGKDIGNTPKNADHGWTVPILQELI
                     KKVAIANPDRKISLVGFSLGATTVRDALRRLWVENQDGTWDVNIFSRVQTVVVASGAN
                     HGVVSFSKQCGLNLTMRGTVTCEMGQRNQYTETAFHRPLNGPPIDGLPEFGGWWETPC
                     ANGDYAFGKTKACGGNKVAYTTITMQDLMNGTQQDEFVSEHASRLYPKECADNQLNGL
                     NDFDTSGYFYNGFFRNHYGAVRSEAGLAKVIAALDKE"
     gene            4285..5598
                     /locus_tag="VFV99_31650"
     CDS             4285..5598
                     /locus_tag="VFV99_31650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063992.1"
                     /translation="MRKLIATTLIALPALAYAEKKDSVIEMPPDQVRTVTNVATVDDA
                     IDSTAPTPAVTDKAPEPAEPVVEKKKDKWIEPYIAIAGGMRLESLHQPDTVDRGTQNP
                     TVAVSRLGLRGGVGEHISFASEFEASLGGPLGYGASVWEGQAAIAIRDQWVRYKRSGF
                     AIAAGRIADPASFDYTSKHVADLLLTDLYTRDPLLYSGADRGNGIIATYDVNQHLTLG
                     GTFHSTNPTGITGTLIIGGKLQPFDRPFYLASSAVGNNQNNLPDQNLHIYFGSPSARL
                     HFDNFEVQSEVQVYTLDTQQAIMDDQTIRGYNLRLGGKAWLETPQGRLTGFANVSRNK
                     NEILDPVDSKYRLPDLFHSYTVSAGVDYDYKKNNGIGFQYAMVDTTEPDLHTRLHFIN
                     LATTYWIEDSLAIALRGSVYAQQISGEMMTTGSRSLFLTAKLLLN"
BASE COUNT          960 a         1859 c         1903 g          940 t
ORIGIN      
        1 aacgtgtcga gcgcgaacac ctcggtcgcg cccttgcccg gcggaatcgc gacgcggcgc
       61 gtctgcatct cgcgcagctc gcggccatcg tcgagcttga tcgcgatacc ggcgacgagc
      121 ccgagcggat cggcgtcggg cacgatcgtg atcgcgtcgg gttcgacaat cgaagagatg
      181 cgcaacggcg cggtgcggcc acgcgcgcgc tcgaacggct cggtgatctt tggcgacgtc
      241 cccgcggcga acttgctgtc ggggtcgagc gcgagcacgc gcgcgaagtg atcttcggcg
      301 accgttgcgc ggccgagccc ggcggcgagc cggcccgcga actggtgcag cgtgacgagc
      361 cggtcgtggt cggcgccgcc ctgtgcgatc gtgcgctcga tgatcgcgag tgccggctcg
      421 tactggagct gggcctcgag cgtcatcgcc tgggcgatcg ggtcttcgtc ggcgcgcgcg
      481 gtcgtcgccg agacggcgag gatcaacgcg atcgcgatag cgcgcacgtg cgcagcttac
      541 cagtgcgcca ggcggctcgc gcggttaggg aaagacgacc gtacccacgt gggcgcgccc
      601 cgatacgacg tggtcttgcg cgcgcgatca gttccaggag agcgtgccct tgttgaagag
      661 cttgcccggc tcgatggtga catcgaacgt cttgatcttg ccgtcctcgc gcacggcctt
      721 gaccgtatgt ttgcccggcg agagtcgcgt gccgatgatc ggcgtctgat ccacatacgt
      781 tccgtcgatg tagatctttg cgaaggggaa cgactcgacg ctgaatctgc ccggcgtgtc
      841 gtcgactttg gcggattgct tgtcgccgcg cggattcgca gtctgcttgt cgccgcgcgc
      901 tttgtcctgc ttcggcttcg ggcgtttgtc ggccttggtc ttctcggcga gcttgtcgaa
      961 gtcggtgatc gcgggcgcgc cggttggcgc cgcatcgggc gcgacgaccg cgacggccgc
     1021 atcggccgcc agcgcaacgt ctccgtctgc gggcagcggc gtctcggcaa ccacgcggtc
     1081 cggtgccttc gccagctgcg agttgcgctg tccctcgcgc accatgctga tcgcgagcac
     1141 cgcgccggtg ccaccgacga cgagcaccgc gagggcgatc acgccgaggc ggcgcttgcg
     1201 cacgacgtcg cgcggtgtcg tcagcatgcg cgacggatcg gcggcggcct tgccaggcac
     1261 ggtgaccgcc gtcgcccgcg agcgcgcatc cgcgatcacc ttcgcttgcc gcgtgcgcat
     1321 cgcggagagc tcggcgccgt gccagtcggc gagtgcggcc gcgatctgat cggcggtcgc
     1381 gacgccgccg tgtggctgca tcgccgtctc gacagccttc gcgagctcgc gggcgtctgc
     1441 gtagcgcttg tccttcgcgc gatcgagcgc cttcgcgatc acgtcggcga gcgccgcggg
     1501 caccgccgga tcgacggcat cggcgcgcgg aatgggttcc tcgaggatcg cgcgcgcggc
     1561 caggtaatcg ctgctgcgct tgaacagtcg cgagtgcgtc gcgagctcga acagcacgat
     1621 gccgagcgcg aacacgtcgc agcggcgatc gagctggtcg ccgagcagct gctcgggcgc
     1681 catgtactgc gtcttgccct tgatcgtgcc ggcctcggtt tccgcgccgc catggacttt
     1741 cgcgatgccg aagtcgagca ccttcacgac acccgccgtc gtcacgaaca agttcgacgg
     1801 cgagacgtcg cggtgcacga gcccgagcaa cgcgccgtcc tcgcccttga gctcgtgcgc
     1861 gtgatgcagc ccggcgcacg cttgcgcgac gatgcccgcg gcgacgcgca gctgcgcgat
     1921 gcgatcggga tcacgcggcc gcgcgatgaa gtcgtggacc ggcacgccgt cgaggtacgg
     1981 catgacgagg aagtactcgg cgccgtcgcg gcccagctcg tgcacctcgc agacgttcgg
     2041 atggtcgaga cgcgcgacga gcttgccttc gtcgaggaac atcgcgacgt acgccttcga
     2101 cgcgacgagc tcgggcaaca ggcgcttgac gacgactgtc ttctcgaagc ccgcttcgcc
     2161 ggcgagccgg gcgaggtgga tctcggccat gccacccgtc gccagctggc cgatcagctc
     2221 gtagcgcccg agacgcgttg gcgtttcggg tgcagcggcc gtttcggcag ggtggtatga
     2281 caaggctgcg tatcatgccg cggatcgcgc tcttgtgcct ggctctcgtc ggagcttgct
     2341 ctttcgacgc cgactacacg agcggtacat actcgtgcag cgacgacaag tgcccgtcgg
     2401 gattggtctg tcagaaccag ctgtgcgtcg ccgagcggag ggatgcggcg gtggacgtgg
     2461 tcgacacgat gcagggcgat gcgagcgatg ggcagatggc cgagctgagc tgcgcgctac
     2521 cgggagcgtt ggctgcgacg ggaggcgccg acagcggtac gacggtggac cgaacgagtc
     2581 atgtcggcgc gcagtgcggt gggttcttga tggccgccgg cgatgcggtc tatcggatca
     2641 cacctggcgc cggaaagcag atgacgatct ccgtttcgac gatgacgact tcgttcatgg
     2701 tctttgccta cgtgacgacg atgtgtcccg ccagcgtgtg ctcgccgaac atggtcgcga
     2761 cgccgggcaa ctcgctcgtg gtgactaccg ttgcaggtcc gcaatacatc gtcgtcgata
     2821 gctatggcgg catgaccggg ccgtacatgc tgaacgtcac cgtcaactga cctgttgtcc
     2881 cgcgccaaca gccgcgctca gtagatgatg gatcgatcca tcaagatcgc tcagtcgtgt
     2941 aggcacttgt gcgggcacgc tggttgctca tgccgcgccc atgctgcgcg gaatgacttg
     3001 cttgctgttg gcagggtgca cgctcggtgg caccggccgt ccgggtgaag gcgacgatac
     3061 gtcccacggt gtcgacgaca ccgcgtgcgg tcaggcgatg gcgaacgcgc cgatcgcgcc
     3121 ggccggctac gactatcacg acgctctgcc gacggcggcg aagaacacgt gggacggcgt
     3181 cacgctgccg cagcccggcg acagtacgta tccgggcggt cgctaccgct cgatcgctgc
     3241 tgacggcgcc ggcaacaaac atcctggctg caccgtggcc ggcctcatgt acacgcccgc
     3301 gaacatcccg ggctacgact gcgcagcgcg ccagttcgat ttccccgatg gcgtcagcga
     3361 agacaccagc aagccgatcg tcatcctcgt gcatggcaac tcggagtcgc cgacggggtg
     3421 ggtgaagttc gtgcacccgg atccgtcgtc gctgatgttc ccggccgaca cgaccgcgcg
     3481 cgatcagctc gccgagaaac tgccggcgct cggcttccgc acgatcgcgg tcgacatgcg
     3541 aaccgacaag atcgacgacc cgaacagccc cgagggcaaa gacatcggca acacgccgaa
     3601 gaatgcggac cacggctgga ccgttccgat cctgcaggag ctgatcaaga aggttgcgat
     3661 cgcgaatccc gacaggaaga tcagcctcgt cggcttctcg ctcggcgcaa cgaccgtgcg
     3721 cgatgcactg cgccgcctgt gggtcgagaa ccaggacggc acgtgggacg tgaacatctt
     3781 ctcgcgcgtg cagacggtcg tcgtcgcgtc gggcgcgaac cacggcgtcg tcagcttctc
     3841 gaagcagtgc ggcctcaacc tgacgatgcg cggcaccgtc acctgcgaga tggggcagcg
     3901 caaccagtac accgagaccg cgttccatcg tccgctcaac ggtccgccga tcgacggcct
     3961 gcccgagttc ggcggctggt gggagacgcc gtgcgcgaac ggcgactacg cgttcggcaa
     4021 gacgaaggcg tgcggcggca acaaggtcgc gtacacgacg atcacgatgc aggacctgat
     4081 gaacggcacg cagcaggacg agttcgtctc cgagcacgcg tcgcggctct atccgaagga
     4141 gtgcgcggac aaccagctca acgggctcaa cgacttcgat acgagcggct acttctacaa
     4201 cggcttcttc cgaaaccact acggcgctgt tcgcagtgaa gcgggcctcg cgaaggtgat
     4261 cgcagctctg gataaggaat gatcatgcgc aagctcatcg ccacgaccct catcgcgctt
     4321 ccggccctcg cgtacgccga gaagaaagat tccgtcatcg agatgccgcc cgatcaggtg
     4381 cggaccgtaa cgaacgtcgc gaccgtcgac gacgcgatcg actcgacggc accaacgccc
     4441 gcggttaccg acaaggcccc ggagccagcg gagcccgtcg tcgagaagaa gaaggacaag
     4501 tggatcgagc cgtacatcgc gatcgcgggc ggcatgcggc tcgagagcct gcatcagccg
     4561 gataccgtcg accgtggcac gcagaatccg accgtcgcgg tgtcgcgact cggcctgcgc
     4621 ggtggcgtcg gcgagcacat ctcgttcgcg agcgagttcg aggcgtcgct cggtggcccg
     4681 ctcgggtacg gcgcgtcggt gtgggaaggg caggcggcga tcgcgattcg cgatcagtgg
     4741 gtgaggtaca agcgctcggg attcgcgatc gcggccggtc gcatcgccga cccggcgagc
     4801 ttcgattaca cgtcgaagca cgtcgccgac ttgttgctca ccgatctcta cacgcgcgac
     4861 ccgctgctct actccggcgc cgaccgcggt aacggcatca tcgcgaccta cgacgtcaac
     4921 cagcacctca cgctcggtgg aacgttccac tcgacgaacc cgaccggcat caccggcacg
     4981 ctgatcatcg gcggcaagct gcagccgttc gatcgtccgt tctatctggc gtcgtcggcg
     5041 gtcggtaaca accagaacaa cctgcccgat cagaacctgc acatctactt tggctcgccg
     5101 tcggcacggc tgcacttcga caacttcgag gtgcagagcg aagtacaggt ctacacgctc
     5161 gacacgcagc aggcgatcat ggatgaccag acgatccgcg gctacaacct gcggctcggc
     5221 ggcaaggcgt ggctcgagac gccccagggg cggctcaccg gcttcgcgaa cgtctcgcgc
     5281 aacaagaacg agatcctcga cccggtcgac tcgaagtatc ggctccctga tctgttccac
     5341 tcgtacacgg tgtcggcggg cgtcgactac gactacaaga agaacaacgg catcggcttc
     5401 cagtacgcga tggtcgacac gaccgagccg gacctgcata cgcggctgca cttcatcaac
     5461 ctggcaacga cgtactggat cgaggactcg ctcgcgatcg cactgcgtgg ctcggtgtac
     5521 gcgcagcaga tcagtggcga aatgatgacg accgggagcc gctccctgtt tctgactgcg
     5581 aagctgctgc tcaactgagc tacactgagc gcgatggcta atgcgctcga gaacgcatta
     5641 cagaaggcgc tgtcggtccg gc
//
LOCUS       DASUFX010000784         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1514932, whole genome shotgun sequence.
ACCESSION   DASUFX010000784 DASUFX010000000
VERSION     DASUFX010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514932"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VFV99_31655"
     CDS             complement(<1..988)
                     /locus_tag="VFV99_31655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5063993.1"
                     /translation="MDAVPALIAYIDRDQRYRVINAAYEKWFGDVRGKQVIEVLGPPG
                     YTNVRDMIERALRGERVSCEGQMIFPDGQRRWIKADYIPDIESTGAVRGYTMQLHDIS
                     EHKQAQERLALLVEASTALAASVDQEDIAQSIVNTVAPALSEWAAVYVDRFGMLVPAA
                     VAHGDGLTTDATWQLARSFVPQATGSLSPDSRQLVVPLVARGKRLAVLVLGATALRAW
                     TFEDILLAEELGRRASASLDVARLFEQQRRANERLQEVDRRKDELLAIVGHELRNPLA
                     PIVTALDVMEYRGLVGCERERAVIRRQTLHMSRLVEDLLDVARVRRGKVALTK"
     gene            complement(1122..2228)
                     /locus_tag="VFV99_31660"
     CDS             complement(1122..2228)
                     /locus_tag="VFV99_31660"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEX5063994.1"
                     /translation="MRAAALIVLASCAVSARDTTPPPPDAGQPDDDASGGGGVPDGAP
                     AACTPQPIGGLTPEQWYWVPVSGSACGNGSSAGFAINTTTKSKEVVIFLMGGGGCYSA
                     ETCSGPQPTAANLNGYTASTAAQELGMFQAGSIFDRNAASNPLRDATFVFVPYCTGDF
                     HSGNTVAAYGTHHVGYANISAYLAMLAPAYCDASRIVLTGSSAGGFGTLFNYEHVQEA
                     FPQTPIDLVDDSGPPLRQVVTQQNQLRAAWGQVANLPADCIGCDQAWHNFLPYLTAKH
                     PAQRFSLISSLQDYSIGTFFGFTPSTYQAAINDLADNVIAPLANLRVYYIANNDHVWL
                     NRNLASISTSGTTLATFLQNELDHTSPFTNIRPP"
     gene            2296..>2796
                     /locus_tag="VFV99_31665"
     CDS             2296..>2796
                     /locus_tag="VFV99_31665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5063995.1"
                     /translation="MRFVLAQRFANVQRHPAGAAARREADAIVSALERATVPTEDDVA
                     LLVDQTFVWIALGLARRARALVERWLSSGLPRVQTLTTSRNQTTGRNQTTSRNQTTGR
                     NRTTGRNRTTGRNRTISGNDAQNLLGIERRLRRAIAIYTVAIAPSAAAGKLRAVADDP
                     IAAAAAI"
BASE COUNT          509 a          900 c          898 g          489 t
ORIGIN      
        1 gcttggtgag cgcaaccttg ccgcggcgga cgcgtgcgac atcgagtaga tcctcgacca
       61 gtcgcgacat gtgcaacgtc tggcgacgga tcactgcgcg ttcgcgttca cagccgacga
      121 gcccgcggta ctccatcacg tcgagcgcgg tgacgatcgg tgcgagcgga ttgcgcagct
      181 cgtggccgac gatcgcgagc agctcgtctt tccggcgatc tacctcctgg agccgctcgt
      241 tcgcgcggcg ctgctgctcg aacaagcgcg cgacatcgag cgacgcactc gcgcggcggc
      301 cgagctcctc ggcaagcaag atgtcctcga acgtccatgc gcgcaatgcg gtcgcaccga
      361 ggaccagcac ggcgaggcgc ttgccgcgcg ccacgagtgg cacgacgagt tgtctgctgt
      421 cgggcgacaa cgagccggtc gcctgtggca cgaacgagcg cgcgagttgc cacgtcgcat
      481 cggtggtcag cccatcaccg tgcgcgaccg cagccggcac gagcataccg aagcgatcga
      541 cgtagacggc tgcccactct gagagcgccg gggccaccgt gttcacgatc gactgcgcga
      601 tgtcctcttg atcgacggaa gccgcgagcg ccgtgctcgc ttcgacgagc aacgcgagcc
      661 gctcttgcgc ttgcttgtgc tcgctgatgt cgtgcagctg catcgtgtaa ccgcgcactg
      721 cgccggtcga ctcgatatct gggatgtagt cggccttgat ccagcgtcgt tgaccgtccg
      781 gaaagatcat ctggccttcg cacgacacgc gctcgccgcg cagtgcgcgc tcgatcatgt
      841 cgcgcacgtt cgtgtagcca ggcgggccga gcacctcgat gacttgcttg ccgcgcacgt
      901 cgccgaacca cttctcgtac gccgcgttga tcacgcgata acgctggtcg cgatcgatat
      961 acgcgatcag cgccgggacc gcgtccacga caacacgaag ttgatcgtcg cttcccacgg
     1021 ctcacgacgg tatcacagcg tttctcgtcg aagattgaca gttagtgcca gcgccattgt
     1081 cacgcgtcgc acaaacgtta ctgcagcgag cgttagctct ctcacggtgg acggatgttt
     1141 gtgaagggtg atgtgtggtc gagctcgttt tgcaagaacg tcgcgagcgt cgtgccgctc
     1201 gtagagatcg acgcgaggtt gcggttgagc cagacgtgat cgttgttcgc gatgtaatag
     1261 acgcgcagat tcgcgagcgg cgcgatcacg ttgtcggcga gatcgttgat cgctgcttga
     1321 tacgtgctcg gtgtgaaacc gaagaacgtg ccgatcgagt agtcctgcaa gctcgagatc
     1381 agcgagaagc gctgcgctgg atgtttcgcc gtcaagtacg gcaggaagtt gtgccacgcc
     1441 tgatcgcatc cgatgcaatc ggccgggagg ttcgcgacct gtccccatgc ggcgcgcaac
     1501 tggttttgct gcgtgacgac ctgacgcagt ggtggtccgg aatcgtcgac gagatcgatc
     1561 ggcgtctgcg gaaacgcctc ctggacgtgc tcgtagttga acagcgtgcc gaagccgccg
     1621 gcgctcgaac cagtgagcac gatgcgtgaa gcatcacagt aggccggtgc gagcatcgca
     1681 aggtacgcgc tgatgttcgc gtagccgacg tgatgcgtcc cgtacgcggc aaccgtgttg
     1741 ccgctatgaa agtcgccggt gcagtacgga acgaacacga acgtcgcgtc gcgcagcgga
     1801 ttgctcgcgg cgttgcgatc gaagatcgag ccggcctgaa acatgccgag ctcctgggct
     1861 gccgtgctcg cggtgtagcc gttcagattc gcggcggtcg gttgcgggcc gctgcaggtc
     1921 tccgcggaat agcagccacc accgcccatc aagaagatca ccacctcctt cgacttcgtc
     1981 gtcgtgttga tcgcgaagcc ggccgacgaa ccgttaccac acgccgagcc cgaaaccggg
     2041 acccagtacc attgctccgg cgtgagcccg ccgatcggct gcggtgtgca cgcggctggc
     2101 gcaccgtcgg gaacgccgcc gccgcccgat gcatcatcat caggctgccc cgcatcaggc
     2161 ggcggcgggg tggtgtcacg cgcgctaacc gcgcacgaag cgagaacgat cagggcagca
     2221 gctcgcatgt cacgagcatt ggcgaatccg gcttccgaca cttgttactc gtactttctc
     2281 cacctttcac gtaatgtgcg attcgtgctg gcgcagcggt tcgccaacgt gcaacgccat
     2341 cccgccgggg ctgccgctcg ccgcgaggcg gacgcgatcg tgagcgcgct cgaacgggcc
     2401 acggttccaa ccgaagacga tgtcgcgctc ctcgttgatc aaaccttcgt ctggatcgcg
     2461 ctcgggttag cgcgccgcgc tcgagcgctt gtcgaacgct ggctgagctc ggggcttccg
     2521 cgtgtccaga cgctcacgac aagccgaaac cagacgacag gccgaaacca gacgacaagc
     2581 cgaaaccaga cgacaggccg aaaccggacg acaggccgaa accggacgac aggccgaaac
     2641 cggacgatca gcggaaacga tgcgcagaat ctcctgggca tcgaacggcg actgcgtcgt
     2701 gcgatcgcga tctacaccgt cgcgatcgcg ccatccgccg cggccggcaa gctgcgcgcc
     2761 gttgctgacg atccgatcgc ggctgccgct gccatc
//
LOCUS       DASUFX010000785         6930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_544654,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000785 DASUFX010000000
VERSION     DASUFX010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544654"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFV99_31670"
     CDS             complement(<1..850)
                     /locus_tag="VFV99_31670"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5063996.1"
                     /translation="MTFALIAALLVLATAIITAAIMRRRIRRMSLAMQDVIREPTKRI
                     PTLSLTGEERALADWINALAEDAERSRNALASERTLLASVADGVTQGVIAIDGERKIE
                     MLNDAARRMLGVTSSLVGEPLLEFVRVPELRALIDRPEDASAEVSLPNGPRTLIRAAR
                     TWGRAGRVLLLEDVTRVRRLESVRRDFVANVSHELRTPVAVIRANAETLIAGAKDDPQ
                     MAPKLIDGLHRNAERLARIIADLLDLSRLDAGQYRLDVSSVPVRTVTEQSLSAIEPQA
                     QKRGIKV"
     gene            complement(847..1557)
                     /locus_tag="VFV99_31675"
     CDS             complement(847..1557)
                     /locus_tag="VFV99_31675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5063997.1"
                     /translation="MSGLVLVIEDEVDLATTLEYNLRSEGFKTRVAHNGKDGLASALA
                     EPVPDIIVLDLMLPDLSGTEICRRLREGDKTRDVPVIMCTAKGEEIDRVVGFEVGADD
                     YVVKPYSVRELMLRIRAQLRHRAQRAEGEPAKLEFGRLEIDRDAHRAWVDKAEVALTA
                     LEFKLLYAFMSRRGRVQSRDSLLSDVWGIDADVTTRTVDTHVKRLREKLGEAGGYIET
                     LRGVGYRFKSDPDEAAQA"
     gene            1636..2754
                     /locus_tag="VFV99_31680"
     CDS             1636..2754
                     /locus_tag="VFV99_31680"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5063998.1"
                     /translation="MSLEDLMPEPLLLMGPGGLAVWQWIGLAVVLVVAAGIGRAGAWL
                     FTWIGAKVVGRTSTNLDDELLAKLRSPLRALASIGLIRIGMVPLALPPTAERIVHEFL
                     LAGFALSLVWGALRSIDVMSSHLGRAGWAVERPSTRSLLLLLSRIAKALVVIIAGIGF
                     LSGIGLPVASLLAGLGIGGIALAFGAQKTVENLFGAVAIGVDRPFREGDFVKVEDHVM
                     GTVEEVGLRSTRFRTADRTIVTLPNGRLSDMRIETYTVRDRIRLACVLNLVYETTAKQ
                     MREILAGCEKVLRDHPALWPDEVSVRFMQLGESSLDVEVIAWFRTTDFGKFKVMRQEV
                     LLGFMQVVEDAGSAFAFPTRTVHLVEQNAAKPRSARRA"
     gene            2782..3141
                     /gene="yhbY"
                     /locus_tag="VFV99_31685"
     CDS             2782..3141
                     /gene="yhbY"
                     /locus_tag="VFV99_31685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEX5063999.1"
                     /translation="MLTGKQRRHLRGLGHELKPIVQIGKGGIDEGLIAALDQALADHE
                     LVKIKIGEAASLDRQEAATELATQTHSEVAQVLGNTVLLYRADPDDPKIVLPRPKRGE
                     PENDNAPEDEGEEDDVQ"
     gene            complement(3167..4429)
                     /locus_tag="VFV99_31690"
     CDS             complement(3167..4429)
                     /locus_tag="VFV99_31690"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5064000.1"
                     /translation="MKLGVMGAVLLVPSIAAADARLFMNAAGEANDLDFVGESRGDGL
                     VMARQVLPAPDQPIVNGLSDGPKQAVLAQSKIIYLNKNGVTLSPGNNDSRTNRSTIVT
                     QTTSIPAWNVTAANWAAVVSCTKDLFARFDVTVVDTDPGNVPHIEAVFGGTPTQVGLP
                     NNVAGVSPFTTDCSIIENSVVFTFAGAFQLTSRDACEIMAQEIAHSYGLDHELLASDP
                     MTYLQYTGNRAFQDQTASCGEDVQRACGINGSTCRANQNSVTLLKERLGTAGDLTAPM
                     LTWQSPSDGATVPPGFEVHASGTDNVAVTGATLKIDGTQVDMKTGAGPFVFVTSATLP
                     EGAHQIVIEITDGKNLQTQTRSVTVKKGAPPPDPGGGSGSGSGTGGGGSDDLNNGDIV
                     GGCAAGGGQAGMLFGLALVFGLARRRRR"
     gene            complement(4610..5920)
                     /gene="pepP"
                     /locus_tag="VFV99_31695"
     CDS             complement(4610..5920)
                     /gene="pepP"
                     /locus_tag="VFV99_31695"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX5064001.1"
                     /translation="MFDPAVFAARRDAYMRAIGPDGVAVVRSLPERLRNGDAFHPFRQ
                     LSDLVYLTGFVEPDTTLVLRPGHDTERVVMFVRPRDPEMETWDGRRAGIDGAKAQYGA
                     DAAYPAAELPAKLGELIANHEELHFAVGLDDEMDLLVTHAIAKLRKTEKRGKRPPRAI
                     VDPRAALHELRLHKRPEELAALRKAAEISSEAHVLAMRAGKPGTYEHELEALINYTFR
                     RHGGAGPGYTTIVGAGQNATILHYIENKCPIANGDLVLVDAGCEYEHYTADITRTWPA
                     SGTFTPIQRRVYQIVLDTQIEAVAMVKPGVTIDEIHNYCVRRLTEGMIDLGLLSGSVD
                     DRVTDQSYRKFYMHGTSHWLGLDVHDVGAYTQGGKARPLEPGMVITVEPGLYVAADAP
                     DVPAELRGIGVRIEDDVVVTAAGHDVLTASCPKKIEDIEAACRG"
     gene            complement(5939..6613)
                     /locus_tag="VFV99_31700"
     CDS             complement(5939..6613)
                     /locus_tag="VFV99_31700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064002.1"
                     /translation="MQATYGIGGYGFLLSPSPATRVMVTEDKELVFFRGEREVARITP
                     NDEPASGDANWLSGSMARYGEGGPLLELADGVEVESVFLRIDTGLPHVEWSGLAAGVV
                     VELPVGVALVPAAPGDADPFFELHAIGARDEFISFMPMQVPVDEIRINPAPYQRLVNQ
                     GKLDDMTFTECAYEHDGKQWRQIFYAVPLQDETSVVVRAQASEPRVELLFQAAQAAAQ
                     TLVPLR"
     gene            complement(6663..>6930)
                     /locus_tag="VFV99_31705"
     CDS             complement(6663..>6930)
                     /locus_tag="VFV99_31705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064003.1"
                     /translation="NGAIALELLKTRSFDAAIVDVYLPVLDGASLIQQARTALGLTKL
                     PIITMSAGGDGARTAALKAGASVFLDKPVRLREVVATMRQLLSV"
BASE COUNT         1133 a         2305 c         2296 g         1196 t
ORIGIN      
        1 cgaccttgat gccgcgcttc tgcgcttgcg gctcgatggc gctcagcgat tgttcggtca
       61 ccgtacgcac cggcaccgag ctgacatcga gccggtactg acccgcgtcg agacgcgata
      121 gatcgagtag gtcggcgatg atgcgcgcga gccgctcggc gttgcgatgc aggccgtcga
      181 tcagcttcgg cgccatctgc ggatcgtcct tcgcgcctgc gatcagcgtc tcggcgttcg
      241 cgcggatcac ggcgaccggc gtgcgcagct cgtgcgagac gttcgcgacg aagtcacggc
      301 gcacgctctc gagccttcgc acgcgggtga cgtcttcgag caggagcacg cggccggcgc
      361 ggccccacgt gcgggcggcg cgaatcagcg tgcgcggccc gtttggcagc gacacctcgg
      421 cgctcgcgtc ttcgggacga tcgatcagcg cgcgcagctc cggcacgcgc acgaactcga
      481 gcagcggttc gccgacgagc gagctcgtca cgccgagcat gcgccgcgcc gcatcgttga
      541 gcatctcgat cttgcgctcg ccatcgatcg cgatcacgcc ttgcgtgacg ccgtcggcga
      601 ccgacgcgag cagcgtgcgt tcactcgcga gcgcgttgcg gctgcgctcg gcatcctcgg
      661 caagcgcgtt gatccagtcc gcgagcgcgc gttcttcgcc ggtcagcgac agcgtcggga
      721 tgcgcttcgt cggctcgcga atcacgtctt gcattgcgag cgacatccgc cgaatgcggc
      781 gtcgcatgat cgcagctgta atgatcgccg ttgccaacac gagcagggcg gcgatcagcg
      841 cgaaggtcat gcctgcgcgg cctcgtctgg atcgctcttg aagcggtagc caacgccgcg
      901 cagcgtctcg atgtagccgc ccgcttcgcc gagcttctcg cgcagccgct tgacgtgcgt
      961 atcgacggtg cgcgtcgtga cgtcggcatc gatgccccag acgtcgctga gcagcgagtc
     1021 gcgtgactgc acgcggccgc ggcgcgacat gaatgcatag agcagcttga actcgagcgc
     1081 ggtcaacgcg acctcggcct tgtcgaccca cgcgcggtgt gcgtcgcggt cgatctcgag
     1141 ccggccgaac tcgagcttcg ccggctcgcc ctcggcgcgt tgtgcgcgat gccgcagctg
     1201 agcgcgaata cgcagcatca gctcgcgcac gctgtacggc ttgacgacgt agtcatcggc
     1261 gccgacctcg aagccgacca cgcgatcgat ttcctcgccc ttcgcagtgc acatgatgac
     1321 cggcacgtcg cgggtcttgt cgccttcgcg caggcggcgg cagatctcgg tccccgacaa
     1381 atccggcagc atcagatcga gcacgatgat gtcgggcacg ggctcggcga gcgccgacgc
     1441 gaggccgtcc ttgccgttgt gggcgacccg ggtcttgaac ccctccgagc ggaggttgta
     1501 ctcgagcgtg gtcgccaaat cgacctcgtc ttcgatcacc aggaccaatc cgctcatacg
     1561 cagcgtttag cacgagccgt gtgacggcag tgtgacggac acgtggccgc cgggaaacgg
     1621 agtatcgtgc gcgcgatgag tctcgaggac ctgatgccgg agccgctgct tctcatgggg
     1681 ccgggcggac ttgcagtgtg gcagtggatc ggccttgcgg tcgttctcgt agtcgccgcg
     1741 gggatcggcc gggcgggtgc ctggctgttt acttggatcg gcgcgaaggt cgtcggtcgc
     1801 acgtcgacaa acctcgatga cgagctgctc gccaagctcc ggtcaccgct gcgcgcgctc
     1861 gcatcgatcg gattgatccg gatcggcatg gtgccgctcg cgctgccgcc aaccgccgag
     1921 cggatcgtcc acgaattcct gctcgcaggg ttcgcactct cgctcgtatg gggcgcgttg
     1981 cgctcgatcg acgtgatgtc gtctcacctc ggccgcgcgg ggtgggcggt cgaacggccg
     2041 agcacgcgct cgttgctgct cctcctcagt cgcatcgcga aggcacttgt cgtgatcatc
     2101 gccggcatcg gctttctcag cggcatcggc ctgccggtcg cgagcctgct cgccggcctc
     2161 ggcatcggcg gtatcgcgct cgcgttcggc gcgcagaaga ccgtcgagaa cttgttcggc
     2221 gccgtcgcga tcggtgtcga ccggccgttt cgcgaaggcg acttcgtcaa ggtcgaagac
     2281 cacgtgatgg gcaccgtcga ggaagtcggt ttgcgctcga cccggttccg cacggccgat
     2341 cgcacgatcg tcacgctgcc gaacggccgg ctctccgaca tgcgaatcga gacgtacaca
     2401 gtccgcgatc ggatccgcct cgcgtgcgtg ctcaacctcg tctacgagac gacggcaaag
     2461 cagatgcgcg agatcctcgc cggctgcgag aaggtactgc gcgatcaccc ggcgctctgg
     2521 cccgacgagg tgagcgtgcg cttcatgcag ctcggcgagt cgtcactcga cgtcgaggtc
     2581 attgcgtggt ttcgcacgac cgatttcggc aagttcaaag tgatgcgcca ggaggtcctg
     2641 ctcggcttca tgcaagtcgt cgaagacgcc ggctcagcgt tcgcattccc gacgcgtacc
     2701 gtgcatctcg tcgagcagaa cgcggctaag ccgcgcagcg cacggcgcgc gtaggcgcta
     2761 caaggtgcta caacccggcc gatgttgact ggaaaacagc gccgccacct ccgaggcctc
     2821 ggccacgagc tcaagccgat cgtgcaaatc ggcaagggcg gcatcgacga gggcctcatc
     2881 gctgcgctcg atcaggcgct tgctgatcac gagctggtga agatcaagat cggcgaggct
     2941 gccagcctcg atcgccagga agccgccacc gagctcgcga cccagacgca cagcgaggtc
     3001 gcgcaggtgc tcggcaacac cgtgctgctc tatcgcgccg accccgacga tcccaagatc
     3061 gtgctgccgc ggcccaagcg cggcgagccc gaaaatgaca acgcccccga ggacgagggc
     3121 gaagaagacg acgttcagta aaaaagggtc agaccgtcag acccctttag cgacgacggc
     3181 gccgcgcgag accgaacacc agggcgaggc cgaacagcat gcccgcctgg ccaccgcccg
     3241 cggcgcagcc accgacgatg tcgccgttgt tgaggtcgtc gctaccgcca ccacccgtgc
     3301 ccgagccgct gcccgagccg ccacctgggt caggcggcgg tgcgcccttc ttcacggtca
     3361 cgctgcgggt ctgcgtctgc aggttcttgc cgtcggtgat ctcgatgacg atctggtgcg
     3421 cgccttcggg cagcgtcgcc gacgtgacga acacgaacgg gccggcgccg gtcttcatgt
     3481 cgacttgcgt gccatcgatc ttgagcgtcg cgccggtgac ggcgacgttg tccgtacccg
     3541 atgcatgaac ttcgaagccg ggcggtaccg tcgcgccatc ggacggcgac tgccaggtca
     3601 gcatgggtgc cgtgaggtcg cctgcagtgc cgaggcgctc cttgagcagc gtcaccgagt
     3661 tctggttcgc gcggcaggtc gagccgttga tgccgcacgc gcgctgcacg tcttcaccgc
     3721 agctcgcggt ctgatcctgg aacgcgcggt tgccggtgta ctgcaggtac gtcatcggat
     3781 ccgatgcgag cagctcgtga tcgaggccgt agctgtgcgc gatttcctgc gccatgatct
     3841 cgcacgcgtc gcgcgaggtg agctggaacg cgccggcgaa cgtgaacacg accgagttct
     3901 cgatgatcga gcagtcggtc gtgaacggcg agacacctgc gacgttgttc ggcaggccga
     3961 cttgcgtggg cgtgccaccg aacaccgctt cgatgtgcgg cacgttgccc gggtcggtgt
     4021 cgacgaccgt gacatcgaag cgcgcgaaca gatccttcgt gcacgacacg accgccgccc
     4081 agttcgccgc ggtcacgttc cacgccggga tcgaggtggt ctgcgtgacg atggtcgacc
     4141 ggttcgtgcg cgagtcgttg ttgcccggcg acagcgtgac gccgttcttg ttgagataga
     4201 tgatcttgct ctgcgcgagg accgcttgct tcgggccatc ggagaggcca ttgacgatcg
     4261 gttggtcggg cgccggcagc acctgccgcg ccatgacgag gccatcacca cggctctcgc
     4321 ccacgaagtc gagatcgttg gcttcgccgg ccgcattcat gaacagccgt gcgtcagctg
     4381 ctgcgatgga ggggaccagg aggacagcgc ccattacgcc gagcttcatg ggcaggacat
     4441 tcttcacgga gtgtgccggc tagggtcaac gatccccatc gaggcattcg cgaggcagtg
     4501 cggctacttg gcgcccaccg agcgatttgc acatatcgcg caatcgcgga gttagctcgc
     4561 gatcctggga ctcgggctgg gggtggcggt gtcgcctccc ccacgtttgt tacccgcgac
     4621 aagctgcttc gatgtcttcg atcttcttgg gacagctcgc cgtcagcaca tcgtgacccg
     4681 cggctgtaac cacgacatcg tcttcgatgc gcacgccgat gccgcgcagc tccgcaggca
     4741 cgtcgggcgc gtcggccgca acgtacagac cgggttcgac cgtgatgacc atgccaggtt
     4801 cgagcggtcg cgccttgccg ccctgcgtgt acgcacccac gtcgtgaacg tcgaggccga
     4861 gccagtgcga cgtgccgtgc atgtagaact tgcgatagct ctgatcggtg acgcggtcat
     4921 cgacgctgcc gctgagcagg ccgagatcga tcatcccttc ggtcagccga cgcacgcagt
     4981 agttgtggat ctcgtcgatc gtcacgccgg gcttcaccat cgcgacggct tcgatctggg
     5041 tgtcgagcac gatctgatag acgcggcgct ggatcggcgt gaacgtgccg ctcgccggcc
     5101 acgtgcgcgt gatgtccgcg gtgtagtgct cgtactcgca gcccgcatcg acgagcacga
     5161 ggtcgccgtt cgcgatcggg cacttgttct cgatgtaatg gaggatcgtc gcgttctgac
     5221 cggcgccgac gatcgtcgtg tagccgggac cagcgccacc gtgacggcgg aacgtgtagt
     5281 tgatcagcgc ctcgagctcg tgctcgtagg ttccgggttt gccggcgcgc atcgcgagca
     5341 cgtgcgcttc gctcgaaatc tcggcggcct tgcggagcgc cgcgagctcc tcgggacgct
     5401 tgtgcaggcg cagctcgtgg agcgcggcgc gcggatcgac gatcgcacgc ggtggccgtt
     5461 tgccgcgctt ctcggtcttg cgcagcttcg cgatcgcgtg ggtgacgagc agatccatct
     5521 cgtcgtcgag accgacggca aagtgaagct cctcgtggtt cgcgatcagc tcgccgagct
     5581 ttgccggcag ctccgcggca ggatacgcgg catcggcgcc gtactgcgcc ttcgcaccgt
     5641 cgatgccggc acgccgaccg tcccaggtct ccatctccgg atcgcgcggg cgcacgaaca
     5701 tcacgacgcg ctcggtgtcg tgaccgggcc tgagcacgag cgtcgtgtcg ggctcgacga
     5761 aaccggtcag gtagacgaga tcagagagct ggcgaaacgg atgaaacgca tcgccgttgc
     5821 gcagccgctc gggcagcgag cgcacgaccg caacgccgtc gggtccgatc gctcgcatgt
     5881 acgcatcgcg tcgggccgca aacaccgccg ggtcgaacat gcccccggta tacctcgttc
     5941 agcgcagcgg aacgagcgtc tgcgccgcgg cctgcgccgc ctggaacaac agctcgacgc
     6001 gcggctcact cgcttgcgcg cggacgacca cgctcgtctc gtcctgaagc ggaactgcat
     6061 agaaaatctg acgccactgc ttgccgtcgt gctcgtaggc gcactcggta aacgtcatgt
     6121 cgtcgagctt gccctggttg acgagccgct gatacggcgc cggattgatg cgaatttcgt
     6181 cgaccggcac ctgcatcggc atgaacgaga tgaactcgtc gcgcgcaccg atcgcgtgca
     6241 gctcgaagaa cggatcggca tcgccgggcg cggccggcac gagcgcgaca ccgaccggca
     6301 gctcgacgac cacgccagca gcgaggcccg accactcgac gtgcggcaac ccggtgtcga
     6361 tgcgcagaaa cacggactcg acctcgacac cgtcggccag ctcgagcagc gggccacctt
     6421 cgccgtagcg cgccatcgaa cccgacagcc agttcgcgtc gccgctcgcc ggctcgtcgt
     6481 tcggggtgat gcgcgcaact tcacgctcgc cacggaaaaa caccagctcc ttgtcctcgg
     6541 tcaccatcac gcgcgtcgcg ggcgacggcg acaacaggaa cccgtagcca ccaatgccgt
     6601 aggttgcttg catacggcgc ggaccatagt gatggcccac gacgtgggtc aacgaaaaag
     6661 tcctaaacgc tgagaagctg gcgcatggtc gcgacgacct cgcgcaatcg cacaggttta
     6721 tcgaggaaca ccgatgctcc cgctttgagt gctgcggtgc gcgcgccatc gccgccggcc
     6781 gacatcgtga tgatcggcag cttcgtgagg ccgagtgcgg tgcgcgcctg ctggatcaaa
     6841 ctggcaccat caagtaccgg taggtagacg tcgacgatcg cggcgtcgaa cgaacgcgtc
     6901 ttgagcagct cgagtgcgat cgcgccgttc
//
LOCUS       DASUFX010000786         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1514956, whole genome shotgun sequence.
ACCESSION   DASUFX010000786 DASUFX010000000
VERSION     DASUFX010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514956"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFV99_31710"
     CDS             <1..444
                     /locus_tag="VFV99_31710"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5064004.1"
                     /translation="GFTRAEKPYLPVIEEGPYGYHNVNAALQKTDPHSLLNWTERMIR
                     ARKEAPELGWGDYEVVETGNAAVLALRYNWRNNSVLTLHNFAGEAIEIELHLGDQAGR
                     KLRSLLTNGRSDADHRGRHHIIMEPYGYHWYRIGGLGYLLDRTSY"
     gene            complement(441..1199)
                     /locus_tag="VFV99_31715"
     CDS             complement(441..1199)
                     /locus_tag="VFV99_31715"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX5064005.1"
                     /translation="MIALTRPVPTSFAAALCAAAPDEAIDVARARSQHSAYRAALAEL
                     GVSVTLLPGDDALPDSCFVEDTAVVAAGIALITRPGAPSRQPETAATAEALRTHFELA
                     YVNAPATLDGGDCMRVGNAIFVGRSARTNAAGIARLAEVFEPRGMRVVAIEMPPAVLH
                     LKCVCAPLGDDRITLADDSIPRAAFGDIHVVRVPAAESYAANVVAIGNRVIAAAGFPR
                     THEALTKAGFSVLPLATTEFRKADGALTCLSIVC"
     gene            1231..3282
                     /locus_tag="VFV99_31720"
     CDS             1231..3282
                     /locus_tag="VFV99_31720"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5064006.1"
                     /translation="MRGLLLVVALVACSAKPKATVPAPPATGGGGDQPTEPIAKPEPA
                     VAEPPVPAAETLAADTPKATVGGATFVAPAGWTFAVRGSATILTPPEGDSHIAFVDVE
                     AKNADQAVALAWAAYSPNGKRWPLLLSNPEAATEGWQEVQSYSYQTSPNEKRIVGVQA
                     FRANNMWTVVVIDVAQAVGEKRGAQFGLIFGQLLPKGYERESFAGKQAHGLDKARIAE
                     LGAWVENAQKQLGVPGVSIGLIQNGKVVFAGGFGVREIGKPAKVDADTKYIIASNTKA
                     MTTLMLAKLVDEKKLTWETAATSLLPQFKLGDADTTSKVLVKHLICACTGLPRQDMEW
                     LLEYGKLTPDGALATLGTMQPTSKFGELFQYSNPLAAAAGFLGGHVAFPKLELGKAYD
                     EAMRTRVFGPLGMTATTLDFKKGQTGNFAVAHSANIDGKTALALTQVNSAVIPVRPAG
                     GAWSTVRDVLKYVQMELAEGKLPNGKQYVTKDTLLARRAPQVAIGKDATYGMGLMVET
                     KYGITVVHHGGDLIGYHSDMIWLPEFGVGAVVLTNGDPGWMIRGGFRRKLLEVLFDGK
                     PEADAELAAGAKTWFEQIATERKQLVVPANADDANKLATKYANDALGEITVTHKGATT
                     TFDFGEWKSEVASKKHPDGSTSFVTIAPGLGVELVVGSANGKRSLVLRDAQHEYVFVE
                     K"
     gene            3420..4040
                     /locus_tag="VFV99_31725"
     CDS             3420..4040
                     /locus_tag="VFV99_31725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5064007.1"
                     /translation="MEGIEPAAQRLLAEIPLRSRVLEIGCGTGAFTRELAVKRGARIT
                     AIDLAPRMIDVARVRTNANLGIEYCVADFMQLSPRGFDVVLSINTLHHLPLAVAAERM
                     AQAVVPGGLVLIADLFDARGVGELPYNGLSWLFRKPRAHDPALAAAWTEHGQHEQQLP
                     LREIRAALRDVLPGVSIRRHLGWRYTAMWRRPPDPTLLARGSTRER"
     gene            complement(4037..>5341)
                     /locus_tag="VFV99_31730"
     CDS             complement(4037..>5341)
                     /locus_tag="VFV99_31730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064008.1"
                     /translation="GVWALAMLDDFDGALAVIATWRHRYPSLPPAHEQALLAAEARVE
                     SIRHHHARELHLLEEALAICDDAGLGFERMYLEASYAIALVRAGELPAANQIVRDWNG
                     DGRHRARAATARNAQARNEPARNAQARNEPARNEQALEVYRDVARTELALLAGDARSA
                     DAAARRVLAYANASGNLVYGCLARFHRVLAATRARRPALLAEYGRAAAQLQIPVHLRR
                     HRLLDELPREARLVVRERRRKHSAPLVRLFYPALTDLGSELCWDRVQGTLYMSGDGPF
                     SLAEHPVIRRVLEVILARSAFELPIATLFEHVWEMSYEPLRHEGKCHVALHRLRALLG
                     GWRTGADRLIEVRDGTVRIADDANICVIELPRAAKPVRATDSLTDRVATYLEAAGEAS
                     PAELAKRIGVSRNALLLVLRGLVAEGRVERIGRARAIRYRAC"
BASE COUNT          935 a         1724 c         1858 g          824 t
ORIGIN      
        1 ggattcacgc gcgccgagaa gccgtatctg ccagtcatcg aggagggccc gtacggctat
       61 cacaacgtga acgcggcgct gcagaagacg gatccgcact cactgctcaa ctggaccgaa
      121 cgaatgatcc gcgcccgcaa ggaggcacca gagctgggct ggggcgacta cgaagtcgtc
      181 gagaccggca acgccgcggt gctcgcgctt cgctacaact ggcgcaacaa cagtgtgctg
      241 acgctccaca actttgctgg cgaagcgatc gagatcgagc tccacctcgg cgatcaagcg
      301 ggacgcaagc ttcgcagtct gctcacgaac ggtcgcagcg atgcagatca ccgcggccgc
      361 catcacatca tcatggagcc gtacggctat cactggtatc gcatcggcgg cctcggctac
      421 ttgctcgacc gcacgagcta ctagcagacg atcgataggc aggtcagagc gccgtcggcc
      481 ttgcggaact cggtcgtcgc gagtggcagg acgctgaagc cggccttggt gagcgcttcg
      541 tgcgtacgcg gaaatccagc ggcggcgatc acgcgattgc cgatcgcgac cacgttcgcg
      601 gcgtagctct cggcagcggg aacgcgcacg acgtggatgt cgccgaatgc ggcgcgtgga
      661 atcgagtcgt cggcgagtgt gatccgatcg tcgccgagcg gcgcgcacac gcacttgagg
      721 tgcagcacgg ccggcggcat ctcgatcgcg acgacgcgca tcccacgcgg ctcgaacacc
      781 tcggcgaggc gcgcgatacc ggcggcgttc gtgcgcgcgg agcgaccgac gaagatcgcg
      841 ttgccgacgc gcatgcagtc gccgccgtcg agcgtggcgg gcgcgttgac gtatgcgagc
      901 tcgaagtgcg tgcgcagtgc ctcggcggtg gcggcggtct cgggttggcg cgaaggcgcg
      961 cccgggcgcg tgatcagcgc aatgccggcg gcgacgaccg cggtgtcctc gacgaagcag
     1021 ctgtcgggca gggcatcgtc gccaggaagc agcgtgacgc tgaccccgag ctccgcgagt
     1081 gcggctcggt aagccgaatg ctgactgcgg gcacgggcga catcgatcgc ttcatcgggg
     1141 gcggcggcgc acaacgcagc cgcaaagctt gtcggaactg ggcgcgtcag cgcgatcatg
     1201 cggcgaccat actgcgctaa ggtccgcggc atgcgtggat tgctgcttgt cgtcgcgctt
     1261 gtcgcctgct ctgcgaaacc gaaggccacc gtgccggcgc cgccggcaac gggaggcggt
     1321 ggtgaccaac cgaccgagcc cattgcgaag ccagagccgg cggtcgccga gccacccgta
     1381 cccgcggcgg agacgctcgc ggctgacacg ccgaaggcga cggttggcgg cgcgaccttt
     1441 gtcgcccctg ctggctggac gtttgcggtg cgcggctcgg cgacgatcct gacgccgccg
     1501 gaaggcgact cgcacatcgc gttcgtcgat gtcgaggcga agaatgccga ccaggcggtc
     1561 gcgctcgcgt gggccgcgta tagcccgaat ggcaagcggt ggccgctctt gctctcgaac
     1621 cccgaagcgg cgaccgaggg ctggcaggaa gtgcaatcgt acagctacca gacgtcaccg
     1681 aacgaaaagc ggatcgtcgg tgtgcaggcg ttccgcgcga acaacatgtg gacggtcgtg
     1741 gtcatcgacg tcgcccaagc ggtcggtgaa aagcgcggcg cccaattcgg gctgatcttc
     1801 ggccagctgc ttccgaaggg ctacgagcgc gagtcgtttg cggggaagca ggcccacggg
     1861 ctcgacaagg cgcgcatcgc agagctcggt gcatgggtcg agaacgcaca gaagcagctc
     1921 ggtgtgcccg gcgtgtcgat cggtttgatc cagaacggca aggtcgtgtt cgcgggaggc
     1981 ttcggcgtgc gcgagatcgg caagccggcc aaggtcgacg ccgacacgaa gtacatcatc
     2041 gcgtcgaaca cgaaggcgat gacgacgttg atgctcgcga agctcgtcga cgagaagaag
     2101 ctgacgtggg agacagcagc cacgagcttg ttgccgcagt tcaagctcgg cgatgcggac
     2161 acgacgagca aggtgctcgt gaaacatctg atctgtgcgt gcaccgggtt gccacgccag
     2221 gacatggagt ggctgctcga gtacggcaag ctcacgcctg acggcgcgct cgcgacgctc
     2281 ggcacgatgc agccgacgag caagtttggc gagctgttcc agtactcgaa tccgctcgca
     2341 gcggcggccg gcttcctcgg cgggcacgtc gcgttcccga agctcgagct cggcaaggcg
     2401 tacgacgaag ccatgcgcac gcgcgtgttc ggaccgctcg gcatgacggc gacgacgctc
     2461 gacttcaaga aaggccagac gggcaacttc gccgtcgcgc actccgcaaa tatcgacggc
     2521 aagaccgcgc tcgcgctcac gcaggtcaac agcgccgtga ttccggtgcg gcctgccggc
     2581 ggcgcgtgga gcaccgtgcg tgacgtgctc aagtatgtcc agatggagct cgccgagggc
     2641 aagctgccga acggcaagca gtacgtcacg aaggacacgc tgctcgcccg gcgtgcacca
     2701 caagtcgcga tcggcaagga cgcgacgtac ggcatgggcc tcatggtcga gacgaagtac
     2761 ggcatcaccg tcgtgcatca cggcggcgat ctgatcggct accacagcga catgatctgg
     2821 ttgcccgagt ttggcgtcgg ggcggtcgtg ctcacgaacg gcgatccggg ctggatgatt
     2881 cgcggcgggt tccgccgcaa gctgctcgag gtgctgttcg atggcaagcc ggaagccgat
     2941 gccgagctcg ctgctggtgc caagacctgg ttcgagcaga tcgcgaccga acgcaagcag
     3001 ctcgtcgtgc ccgcgaacgc ggacgatgca aacaagctcg ccaccaagta cgcaaacgat
     3061 gcactcggcg agatcaccgt gacgcacaaa ggcgcgacga cgacgttcga cttcggcgag
     3121 tggaaaagcg aagttgcttc caagaagcat ccggacggct cgacgtcgtt cgtgacgatc
     3181 gcgcccggcc tcggcgtcga gctggtggtc ggttccgcaa acggcaagcg cagcttggtg
     3241 ttacgcgacg ctcagcacga gtacgtcttc gtcgagaagt agagggtgta gcccgctacc
     3301 gatccgctac ccgaccaggg aaatagcctg gcgccaacgt gcgttgcacg gtggagatgc
     3361 cgagtagtgt ggacgtgata cgggcagatt tcgatcggat cgcgagccac tccaccgccg
     3421 tggagggtat cgagcccgcc gcacagcgct tgctcgcgga aatcccgctg cgtagccgcg
     3481 tgctcgagat cggttgtggc accggcgcct tcacgcgcga gctcgcggtg aagcgcggcg
     3541 cgcgcatcac ggcgatcgat ctcgcgccgc gaatgatcga tgtcgcgcgc gtgcgcacga
     3601 atgcaaacct cggtatcgag tactgcgtcg ccgatttcat gcagctgtcg ccgcgcggct
     3661 tcgatgtcgt gctgtcgatc aacacgctgc atcacttgcc gcttgcggtc gctgccgaac
     3721 gaatggcgca ggcggtggtg cccggcggtc tcgtgctgat cgccgatctg ttcgatgcgc
     3781 gcggtgtcgg cgagctgccg tacaacggtc tctcgtggct gttccgcaag ccgcgcgcgc
     3841 acgatcccgc gctcgccgcg gcgtggaccg agcacggcca acacgaacag cagctgccgc
     3901 tgcgcgagat ccgcgcggcg ttgcgtgatg tgcttcccgg cgtgtcgatt cgccggcatc
     3961 tcggctggcg ctacacggca atgtggcgcc gcccgccgga cccaaccttg ctcgcacgcg
     4021 gcagcacgcg cgagcgctaa caagcccggt agcggatcgc gcgcgctcga ccgatgcgct
     4081 cgacgcggcc ctcggcgacg agcccgcgca gcacgagaag cagcgcgttg cgcgacacgc
     4141 cgatccgctt ggcgagctcg gccggagagg cctcgccggc cgcttcgagg tacgtcgcga
     4201 cgcgatccgt gagcgaatcg gtcgcgcgga caggtttcgc agcgcgcggc agctcgatga
     4261 cgcatatgtt cgcgtcgtcc gcgatccgga ccgtcccatc gcggacctcg atcagccgat
     4321 ccgcgccggt gcgccagccg ccgagcagcg cgcgcaggcg atgcagcgcg acgtggcact
     4381 tgccttcatg acgaagcggc tcgtagctca tctcccagac gtgctcgaac agtgtcgcga
     4441 tcggcagctc gaacgccgat ctcgcgagga tgacctcgag cacacgccgt atgaccggat
     4501 gctcggcaag cgagaacggg ccatcgccgc tcatgtacaa cgtgccttgc acgcggtccc
     4561 agcacagctc gctgccgaga tcggtgagcg cgggatagaa cagacggacg agcggcgcgc
     4621 tgtgcttgcg gcgccgctcg cgcacgacga gccgggcctc gcgcgggagc tcgtctagca
     4681 agcggtggcg acgcaaatgg acgggaatct ggagctgcgc ggccgcacgt ccgtactcgg
     4741 cgagtagagc agggcgcctc gcgcgcgttg cagcgagcac gcgatggaac cgtgcgaggc
     4801 agccatacac gaggttgccg ctcgcgttcg catacgcgag gacgcgccgg gccgcggcat
     4861 cagcactgcg ggcatcgccg gccaatagcg cgagctccgt gcgtgcgacg tctcggtaga
     4921 cctccagcgc ctgttcgttc cgcgccggtt cgttccgcgc ttgtgcgttt cgtgccggtt
     4981 cgttccgcgc ctgtgcgttt cgtgcggtgg cggcgcgcgc tcgatgacgc ccgtcaccat
     5041 tccagtcacg gacgatctga ttggccgcgg gcagctcgcc cgcgcggacg agcgcgattg
     5101 catagctcgc ttccaggtac atgcgctcga agccaagacc ggcgtcgtcg cagatcgcga
     5161 gcgcttcttc aagcaggtgg agctcgcgcg cgtggtggtg acgaatgctc tcgacgcgcg
     5221 cttcggcggc gagcagcgcc tgttcgtgcg cgggcggcag gctcggatag cgatggcgcc
     5281 aggttgcgat gaccgcgagc gcgccatcga agtcgtcgag catcgcgagc gcccagactc
     5341 c
//
LOCUS       DASUFX010000787         6144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2072456, whole genome shotgun sequence.
ACCESSION   DASUFX010000787 DASUFX010000000
VERSION     DASUFX010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072456"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFV99_31735"
     CDS             <1..243
                     /locus_tag="VFV99_31735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064009.1"
                     /translation="EPPPPERIDLKIPGAAPTPPGALRAADLPASVMRAFAVKYPHTI
                     PAGANFEGGNYTVLFPAGREHKRAVFTPDGAFISED"
     gene            complement(375..2933)
                     /gene="sppA"
                     /locus_tag="VFV99_31740"
     CDS             complement(375..2933)
                     /gene="sppA"
                     /locus_tag="VFV99_31740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX5064010.1"
                     /translation="MKIAVSVVVIAGVGAVVAPVLAGFAVAHAQSVDRRYAEEPTGGL
                     ELPTTPLAGEYDARTVVTNPAGLPLLRGPELALALDLQDSDVANSAGPGFGAYIATSG
                     GGTILPRYGLGFGFEWLRPSRAQLAPDPGEPFRFTLGLGLALGASSGFGFSWHHFSDD
                     GVLNAVDTFDLGLSSRWGSHFAVGATLLDVSTGAIGGTPVQRRYELETLARPLGTDRL
                     DIAVGARLGETRHDLDGWGRVTVRAARGVYIHGEVETRELYALETSPMGTREDQTRDM
                     RATLGLEISFGSFGMSAFGTGLRNDKGDNHMLGGTLVLRSSALGPASVLGTSSHIERV
                     ELAGNIDSRELTGIVVRLRAIAHDSTAKGVVVMFDGVNAGWATLQELRSELLAVRKTG
                     KKVFAYMVSGTGRDYYVASAADKIYVDPAGGVRLVGIAGTTIYFKGAFDQLGVQPQFE
                     KIAEYKSAPEQFTEAHATETAAKMHNDLYDSLWDQWVSDVAEGRHLSKDEVKALVDNG
                     PYTAGDLDKDKKLVDAVAAPDKVSQLVMAELGGVYPVGRPSPARPDRWTRPGVAVIYV
                     EGDITDGKSQTIPLIGRDLAGGETLVNAITAARNDPRVGAIILRIDSPGGSALASELV
                     SREVFQTRGVKPIICSFSDLAASGGYFIAAGCDVIFAEPMTITGSIGIFFGKFDLSGL
                     LKKYGVTTDTYKRGKRSDVESYFRPYTDEERAMLMEKLRYMYSRFVGAVAAGRPLTKD
                     QVDGLGRGHVYTGAAAKPIQLVDKFGGLGDALDEAKRRMHISTSTKVTLYELPKQSQG
                     LLGMLGNLTRAQEKPSVQLFDLPVIRELVRGVPGSVLVAPDVPQARLPFDISFE"
     gene            2961..3425
                     /locus_tag="VFV99_31745"
     CDS             2961..3425
                     /locus_tag="VFV99_31745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5064011.1"
                     /translation="MQNPTLLERHPLLVNLTADQLARVSSAGDVESYNPNEQIVVEGS
                     LGDALFLILSGQVAVHRGNQTFATLGAGEFFGEMSLVEPAPRSATVTSMQATFLFRLP
                     HDALRDMITQDAGVASILLVQVVKTLSERLRRANQMLSSVDLLADWLAGSMV"
     gene            complement(3422..3724)
                     /locus_tag="VFV99_31750"
     CDS             complement(3422..3724)
                     /locus_tag="VFV99_31750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064012.1"
                     /translation="MRGFRAALLSCAALAAGCDDNRCGHPDELTYDCAPVPLGTANSC
                     SGGPTWEGVHADPDKAYPLGCVASYPFCAAYYPDEVQTCSCTDFGGSGSPTWACPI"
     gene            complement(3727..>6144)
                     /locus_tag="VFV99_31755"
     CDS             complement(3727..>6144)
                     /locus_tag="VFV99_31755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064013.1"
                     /translation="WLASNAPTTDGTRMSTFLQDLFSEDIVRERAERAEMLARVRTRA
                     LTMPPTDELRLLIEKSDKITIEDIAKAAEGDAPVRKPGSVGRRASDKGDQGNDRRRES
                     DRRLPGKTAAKANESGTLALAAQQEEAPELGIGSEIDGRYRVVELIGEGGMGKVYLAE
                     HIEIGKRVALKVLHPSYSRMPDLVERFRREARAASKIGHPNIVDVTDSGATADGSVYF
                     VMEYLEGVELGSVIEREGALDIARALRISGQICRALQAAHREGIIHRDLKPENIFLIT
                     RGGEADVVKVLDFGIAKTTEAEAARERRLTSPGMAMGTPEYMAPEQAAGRPADARTDV
                     YSLGAIMYEMVTGVPPYQGDNFMEILTKKATLDPPAPVSIRSDLPEPVSDLVLAAMSR
                     NPDSRPQSMESLEYELNKCLSGRGQAVASILGMTTDANVVATLNPGLSTRNLDSGIVH
                     ASAARAGTHDPIWDTRSGVSRNSFHSGPTHMAHPTPSGPVRAPSDPGLAVSPASSGKI
                     PRVATPLPVANVSSAPIVNEILSAPGATPEIKSKSGFAVFGWIMLILILLGGGGALAY
                     MMLYKKEPTSSAPVIDNGSNKDGSAQQTNPNEHDDGKDAVVPKDGKHDKGSAEKPSEK
                     GSAETKVTDTKPPTETAKGSNSEKKDPASEKKDPKKTNKAVAALDDKTPWKAISDVAK
                     KAEGAGDWDAALLAYQKLEKAKGYQYPGWAVYKQAWAAFQLNDTNTALNLSQRASTMP
                     GNQKPDAKLLYADALYKQGDYKRAKDFYIGLRKQFNGEPKKQAQVAKKITACNQKLKL
                     PDNDGLK"
BASE COUNT          928 a         2107 c         2038 g         1071 t
ORIGIN      
        1 gaaccgccgc cgcccgagcg catcgacctc aagattcccg gtgcagcccc taccccgccc
       61 ggcgcgctgc gcgcggcgga tctgccggca tcggtgatgc gcgcgttcgc ggtgaagtac
      121 ccgcacacga ttccggccgg cgcgaacttc gaaggcggaa attacacggt gctgttccca
      181 gccggccgcg agcacaagcg cgcggtgttc acgcccgacg gcgcgttcat ttccgaagac
      241 tgagccaccg ctcgggtcgt acacgaagag cgtccgcacc gacgacagct tcggagtggg
      301 cgaagcccaa gcgcttcgca gcggcgacga gcgtgtttcg ccgtgcacgt gcacgtgtac
      361 gattcggacc gacgctattc gaagctgatg tcgaacggca gccgcgcctg cggcacgtcg
      421 ggcgcgacca gcaccgagcc cggcacgccg cggacgagct cgcgaatcac cggcagatcg
      481 aacagctgca cgctcggctt ctcctgcgcg cgcgtcagat tgccgagcat gccgagcaag
      541 ccctgcgact gcttcggcag ctcgtagagc gtcaccttcg tcgacgtcga gatgtgcatg
      601 cggcgctttg cttcgtcgag cgcatcgccg agcccgccga acttgtcgac gagctggatc
      661 ggcttcgcag cggcacccgt atacacgtga ccgcggccga ggccatcgac ttggtccttc
      721 gtcagtggcc ggcccgccgc caccgcgccg acgaaccggc tgtacatgta gcgcagcttc
      781 tccatcagca tcgcgcgctc ctcgtcggtg tacgggcgga agtagctctc gacgtcgctg
      841 cgcttgccgc gcttgtacgt gtccgtcgtg acgccgtact tcttgagcag gccgctgaga
      901 tcgaacttgc cgaagaagat gccgatcgac ccggtgatcg tcatcggctc ggcgaagatc
      961 acgtcgcagc cggccgcgat gaagtagccg cccgacgccg cgaggtccga gaacgaacag
     1021 atgatcggct tcacgccgcg cgtctggaac acctcgcgtg acacgagctc ggaggcgagc
     1081 gcgctgccgc caggcgaatc gatgcgcagg atgatcgcgc cgacgcgcgg gtcgttgcgc
     1141 gccgccgtga tcgcgttgac gagcgtctcg ccaccggcga ggtcgcgacc gatgagcgga
     1201 atcgtctgcg acttgccatc ggtgatgtcg ccctcgacgt agatgacggc gacaccgggg
     1261 cgcgtccagc ggtcggggcg ggcgggcgac ggccggccga ccggatacac accgccgagc
     1321 tcggccatca cgagctggct gaccttgtcg ggagccgcga cggcatcgac gagcttcttg
     1381 tctttgtcga gatcgccggc ggtgtagggg ccgttgtcga cgagcgcctt cacttcgtcc
     1441 ttgctgagat ggcgcccctc ggcgacgtcg ctcacccact gatcccacag cgagtcgtag
     1501 aggtcgttgt gcatcttcgc ggcggtctcg gtcgcgtgcg cctcggtgaa ctgctccggc
     1561 gcgctcttgt actcggcgat cttctcgaac tgcggctgca caccgagctg atcgaacgct
     1621 cccttgaagt agatcgtcgt accggcgata ccgacgagcc gcacaccacc ggcgggatcg
     1681 acgtagatct tgtcggcggc cgatgcgacg tagtagtcgc gcccggtccc cgagaccatg
     1741 tacgcgaaca ccttcttgcc ggtcttgcgg acggcgagca gctcgctgcg cagctcctgc
     1801 agcgtcgccc aaccggcgtt gactccgtcg aacatcacga cgacgccctt cgccgtcgag
     1861 tcatgcgcga tcgcgcgcag ccgcacgacg ataccggtca gctcgcgcga gtcgatattg
     1921 ccggccagct cgacgcgttc gatgtgacta ctcgtgccga gcaccgacgc gggtccgagc
     1981 gccgatgatc gcaggacgag cgtgccgccg agcatgtggt tgtcgccctt gtcgttgcgc
     2041 agcccggtgc cgaacgcact catgccgaag ctgccgaacg agatctcgag accgagcgtc
     2101 gcgcgcatgt cgcgggtctg gtcttcgcgc gtgcccatcg gcgatgtctc gagcgcgtag
     2161 agctcgcgcg tctcgacttc gccatggatg tacacgccgc gcgcagcgcg caccgtcacc
     2221 cgtccccagc cgtcgagatc gtgacgggtt tcgccgagcc gtgcaccgac tgcgatgtcg
     2281 aggcggtcgg tgccgagtgg acgcgcgagt gtctcgagct cgtagcgccg ctgcaccggc
     2341 gtgccgccga tcgcgccggt cgacacgtcg agtagcgtcg cgccgactgc gaagtggctg
     2401 ccccagcgcg acgacaggcc gaggtcgaac gtatcgaccg cattcagcac gccgtcgtcg
     2461 ctgaagtggt gccacgagaa cccgaagccg ctgctcgcgc cgagcgcgag gcccaggccg
     2521 agcgtgaagc gaaacggctc gcccggatcc ggcgcaagtt gtgcacgcga cgggcgcagc
     2581 cactcgaagc caaacccgag gccgtagcgc ggcaagatcg tgccgccgcc cgaggtcgcg
     2641 atgtacgcac caaagccggg gccggcgctg ttcgcgacgt ccgagtcctg aaggtcgagc
     2701 gcgagcgcga gctccgggcc acgcaatagc ggcagccctg cgggattggt aacgacggtc
     2761 cgcgcgtcgt actcgccggc gagcggggtc gtcggtagct cgaggccgcc ggtcggctcc
     2821 tcggcgtagc ggcgatcgac cgactgggcg tgcgcgaccg cgaagcccgc gagcacgggg
     2881 gcgaccaccg cgcccacacc cgcgatcacg acgacggaga ctgcgatctt catcggccgc
     2941 gactatatac tgacgacggt atgcagaacc cgaccttgct ggagcgccat ccactactgg
     3001 tcaatctcac cgccgaccag ctggcacggg tatcgagcgc gggagacgtc gagagctaca
     3061 accccaacga gcaaatcgtg gtcgaaggca gcctcggcga cgcgctgttc ttgatcctgt
     3121 ccgggcaagt tgccgtacac cgcggcaacc agacgttcgc gacgctcggc gcaggagaat
     3181 tcttcggaga gatgtcgctc gtcgagcccg caccgcgctc ggccaccgtg acgtcgatgc
     3241 aggcgacgtt cttgttccgg ctcccccacg acgcgcttcg cgacatgatc acgcaggacg
     3301 ccggcgtagc gtcgatcctg ctcgtgcagg tcgtcaagac gctctccgag cgcctgcgcc
     3361 gcgcgaacca gatgctatcg agcgtcgacc tgctcgccga ctggctcgcc ggctcgatgg
     3421 tctagatcgg gcaggcccac gtcggcgagc cagaaccgcc gaagtccgtg caggagcagg
     3481 tctggacctc gtctgggtag tacgccgcac aaaacggata cgacgctacg cagccgagcg
     3541 gatacgcctt atcgggatct gcatgaacgc cctcccacgt cgggccaccg ctacagctgt
     3601 tcgcggtgcc aagcggtacg ggcgcgcagt cgtaggtgag ctcgtcggga tgtccgcatc
     3661 tgttgtcgtc gcagccagcc gcgagcgccg cgcaggacag caaggccgct cggaagccgc
     3721 gcatcgctac ttgaggccgt cgttgtccgg tagcttcagc ttctgattgc aggccgtgat
     3781 cttcttcgcg acctgcgcct gcttcttcgg ctcgccgtta aactgcttgc gcaggccgat
     3841 gtagaagtcc ttcgcgcgct tgtagtcgcc ctgcttgtac agcgcgtcgg cgtagagcag
     3901 cttcgcgtcg ggcttctggt tgcccggcat cgtcgacgcg cgctgcgaca ggttcagcgc
     3961 ggtgttcgtg tcgttgagct ggaacgcagc ccacgcctgc ttgtacacgg cccagcccgg
     4021 atactggtag cccttcgcct tctcgagctt ctggtacgcg agcagcgccg cgtcccagtc
     4081 gccggcgcct tccgccttct tcgcgacgtc cgaaatcgcc ttccacggcg tcttgtcgtc
     4141 gagcgcggcg accgccttgt tcgtcttctt cggatccttc ttctcgctcg ccggatcctt
     4201 cttctcgctg ttgcttccct tggcggtttc cgtcggcggc ttcgtgtcgg tgaccttggt
     4261 ctccgcactg cccttctcgc tcggcttctc cgcgctgccc ttgtcgtgct tgccgtcctt
     4321 cggcacgacg gcgtccttgc cgtcgtcgtg ctcgttcggg ttcgtctgct gggccgagcc
     4381 gtccttgttg ctgccgttat cgataacagg cgccgacgac gtcggctcct tcttgtacag
     4441 catcatgtac gcgagcgcgc cgccgccgcc gagcaggatc aggatcagca tgatccagcc
     4501 gaacaccgcg aagccgctct tgctcttgat ctcgggcgtc gcgcccggcg cggaaagaat
     4561 ctcgttgacg atcggcgccg acgacacgtt ggccaccggc agcggcgtcg ccacgcgggg
     4621 aatcttgccg ctcgaggccg gcgacacggc gaggcccgga tcgctcggcg cacgcaccgg
     4681 accgctcggc gtcggatgcg ccatgtgcgt cgggccgctg tggaacgaat tgcgcgagac
     4741 accagaccgg gtatcccaga tcggatcgtg cgtaccggcg cgcgccgccg aggcgtgcac
     4801 gatgccgctg tcgaggttgc gcgtcgacag acccggattg agcgtcgcga cgacgttcgc
     4861 gtcggtcgtc atgccgagga tcgacgcgac ggcctgaccg cggcccgaca gacacttgtt
     4921 gagctcgtac tcgagcgact ccatcgactg cgggcgcgaa tcgggattgc gcgacatcgc
     4981 tgcgagcacg aggtccgaga ccggctcggg caggtcgctg cgaatcgaca ccggcgccgg
     5041 cggatcgagc gtcgccttct tggtcagaat ctccatgaag ttgtcgccct ggtagggcgg
     5101 cactccagtg accatctcgt acatgatcgc gccgagcgag tagacgtcgg tgcgcgcgtc
     5161 ggcgggacga cccgccgctt gctccggcgc catgtactcg ggcgtgccca tcgccatgcc
     5221 ggggctcgtg aggcgacgct cgcgcgcggc ctcggcttcg gtcgtcttcg cgatgccgaa
     5281 gtcgagcacc ttcacgacgt cggcttctcc gccgcgcgtg atcaggaaga tgttctcggg
     5341 cttgagatcg cgatggatga tgccttcgcg atgggccgcc tgcagcgccc ggcagatctg
     5401 gccggagatg cggagcgcac gcgcaatgtc cagtgcgcct tcgcgctcga tcaccgagcc
     5461 gagctcgacg ccctcgaggt actccatgac gaagtacacg ctgccatcgg cggtcgcacc
     5521 ggagtcggtg acgtcgacga tgttcgggtg accgatcttc gatgccgcgc gcgcctcgcg
     5581 gcggaaccgc tcgacgaggt cgggcatgcg cgagtagctc ggatgcaaga ccttcagcgc
     5641 gacgcgctta ccgatctcga tgtgctcggc gaggtagacc ttgcccatgc cgccttcgcc
     5701 gatcagctcg acgacgcggt agcggccatc gatctcggaa ccgatgccga gctcgggcgc
     5761 ttcttcttgc tgtgcggcga gcgcgagcgt gccgctctcg ttcgccttcg cggcggtctt
     5821 gcctggcaag cgccgatcgc tctcgcgccg gcggtcgttg ccctggtcgc ccttgtcgct
     5881 cgcgcggcgg ccgaccgagc ccggcttgcg caccggcgcg tcgccctcgg ctgccttcgc
     5941 gatgtcctcg atcgtgatct tgtcggattt ctcgatgagc aggcggagct cgtcggtcgg
     6001 cggcatcgtc agtgcgcgcg tgcggacgcg ggcgagcatc tcggcgcgct cggcgcgttc
     6061 gcgcacgatg tcctccgaga acaggtcctg gaggaacgtc gacatgcgcg tgccgtcggt
     6121 cgtaggcgcg ttcgatgcga gcca
//
LOCUS       DASUFX010000788         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1100047, whole genome shotgun sequence.
ACCESSION   DASUFX010000788 DASUFX010000000
VERSION     DASUFX010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100047"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="VFV99_31760"
     CDS             <1..1990
                     /locus_tag="VFV99_31760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064014.1"
                     /translation="IAISAARTRSAAVAPPAAPKPSLPTVDPDRYQPRDTLADLVAHG
                     VPAAPGAGLSAQLASSPFAPALRHMLPLGIAPSFDVRALFGAGLASTYLAGLIAADTS
                     ELSIAAPLTRAAADFEPTYVAPERPVTDDAGELPSYAEPLTTLRSALLSFDVASSGEP
                     VTQTITATASAPLARTMTEAMALPMFGSMDADAEAAAYAAPGMIAERAQAWSVAQERS
                     SADLAFDFVTPELVLAARVYGLGPAEAAQAARLAVGGPGHLSAMAGTVDRTFVQAMAI
                     EAERRGDRTITAYPAASDATSNASATTFAPSRAAFGVERRAPRGAFLWPAATTGALGM
                     NAAAPDGDHSMSVAALELLAAQAVAELGTYTALSDVERTASATPTTGAAPSEARDEDV
                     LEQASALVPASRRDKFQALYVALSGSTFGPAGRTASPAARAARALALAGRGEDTISAR
                     ERATVAWDVLPVVYGVDAGDEPLSTGDAAARAVRRRDEMRALDPIFVDSRPGLASLSA
                     RAGEALGSYVASPAPAPSSASSSSSSREVGAVLRPPTAAPEFVQTGRPSGRTGGGEVE
                     IPAWFEQAARKMFESKSNVSDGISIAELTLVSTAPAPQVAASTRVSGGAPPVAVAPSA
                     AVGAEKQAIDIEKVANDVYREVLVLMDLARARNGEPYQ"
     gene            1987..2559
                     /locus_tag="VFV99_31765"
     CDS             1987..2559
                     /locus_tag="VFV99_31765"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064015.1"
                     /translation="MSNGNNKNVDGSTFAPVGGGATSSNNAKAGKIMIGSLDDPSIYV
                     EAQYNPKELEISKTVPWSKVNEANKANQKASQGQGIHMEFTGAEGRSLTVEMLFDCYE
                     DDDKTKVAKSIEKLEKLASVRVPGSPKEENKRPHRCMVVWGDVVKMSCVIDGLGVKYT
                     MFSSDGKPLRATCSVKLKEADVVSAKKAGK"
     gene            2563..3648
                     /locus_tag="VFV99_31770"
     CDS             2563..3648
                     /locus_tag="VFV99_31770"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEX5064016.1"
                     /translation="MADGTAAPNYTIKIGTRDATELKTDFLSFVIERDMFQPDMAAIV
                     LSNQAGQYSTIKIADEVDICVGDDAGTSIYKGEVVGLEPVYKGGEKSRILIRAMNKFH
                     RLLRHCKSVTFTDKTDQQILNQVVGDAGLSLSWQHEKSITYKHVYQHNQTDMEFLRTR
                     AARMGCHVWCVDKTIYVKEPNLQQGPAVTLNISEGGGSSDQPTIKSFTPRLSSASILK
                     KVTVKGWNPETKELIVGDYSAKDSKLGDTNAVSASAELGKEETFTVDHPVWSKEEAVA
                     LAKARLVDLSLSFITGECEVTGTTKLDLGTVIGIQANTDPDTKGDDPFNGNYYVMGLT
                     HRFVLSKTKEGGFTTILRLARDAQKKG"
     gene            3651..4373
                     /locus_tag="VFV99_31775"
     CDS             3651..4373
                     /locus_tag="VFV99_31775"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX5064017.1"
                     /translation="MRAGGPRRDSLDHLQYGVHYGIVVQNKDPENLDRVKVRLPWLDS
                     GDVDQSHWAQLLTPMEGKKFGWYTLPDIDDVVAVMFINGDTSQPVIVGGIWSKPDFSP
                     ETNEDGKNNFRGYRSRAGHRLILDDTAKTKVVFADKTTKNMIGIGNFAKDGAGPNVCA
                     VYKPPMSGDVGVSISSMEGTMEITCKDGALKVSAGQDIKINAKTTVDVKAGGDLTAEG
                     SSAAKLTSSDNSNYDGSKVDIA"
     gene            4370..>5699
                     /locus_tag="VFV99_31780"
     CDS             4370..>5699
                     /locus_tag="VFV99_31780"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX5064018.1"
                     /translation="MTLFPIDRPMETALRAAKMRGVHLAIVVENKDGGDNPGYRVCVK
                     YPWMNEQEKSFWARIAIPMAGKERGTYFLPEVDDQLLVVFEHGDINKPIVIGALWSKK
                     QEPVEINESGKNNTKLIKSRAGHRIIFDDKDGAEKITIVDKTKKNKIVLDSANKVVKI
                     ESAGDIEVKAAANVIMHSNALKVGTSDTFTAKGQQVLTHSGAAFGVKASSNITVNGAQ
                     VQINVVNSPAARVSGSGAGELGGAAAETPKDQVQEQTRGGGGGGGGGGAGGGGGGGPA
                     PAAPEPAPSSSSSGGGSSSGGSGSSSAGTAGSPGGASGQPGGGQGGASGSASASATAS
                     GGASGSAQGGAAGGAQGGASGSASAGATASGGASGSAQGGAGGGAQGGASGSASAGAT
                     ASGGASGSAQGGAGGGAQAGASGSAGAGATASGGASGSAQGGAGAQGGAQA"
BASE COUNT         1089 a         1784 c         1985 g          841 t
ORIGIN      
        1 gatcgcgatt tcggcggcgc gcacccgctc ggctgcggtc gcgccgccgg cggcaccgaa
       61 gccgtcgctg ccgacggtcg atcccgatcg ctatcagccg cgcgacacgc tcgccgatct
      121 cgtcgcgcac ggtgtgcccg ccgccccggg tgcaggcctg tccgcgcagc tcgcgtcgtc
      181 gccgttcgcg ccggcgctgc gccacatgtt gccgctcggt atcgcgccga gcttcgacgt
      241 gcgcgcactg ttcggggccg gcctcgcatc gacgtacctg gcaggcctca tcgccgccga
      301 cacgagcgag ctgtcgatcg ccgcgcccct aacgcgcgct gcggccgact tcgagccgac
      361 gtacgtcgcg cccgagcgcc cggttaccga cgatgctggc gagctaccaa gctacgccga
      421 gccgctcacc acgctgcgca gcgcgctgct ttcgttcgat gtcgcgtcga gcggtgagcc
      481 tgtaacacag accatcaccg caacggcgag cgcgccgctc gcacgcacga tgaccgaagc
      541 gatggcgctg ccgatgttcg ggtcgatgga tgccgatgcc gaagcggcgg cctacgcggc
      601 gcccggcatg atcgccgagc gtgcccaggc gtggtcggtc gcgcaggaac gatcgtccgc
      661 ggatctcgcg ttcgacttcg tgacgcccga gctcgtgctc gccgcgcgcg tctacggcct
      721 cggccccgcg gaagcggcgc aagcggcgcg tctcgcggtc ggtggtccgg gccatctcag
      781 cgcgatggca ggcaccgtcg atcgcacgtt cgtgcaagca atggcgatcg aagccgagcg
      841 ccgtggcgac cgcacgatca ccgcgtatcc ggccgcgagc gatgcgactt cgaacgcgag
      901 cgcgacgacg ttcgcgccga gccgcgccgc attcggagtc gagcgtcgtg caccgcgcgg
      961 tgccttcctg tggcccgcag cgacgaccgg ggccctcggc atgaacgccg ccgccccgga
     1021 tggcgatcac tcgatgtcgg tcgctgcact cgagctgctt gctgcacagg ccgtcgccga
     1081 gctcggcacg tacaccgcac tgagtgacgt cgagcgcacc gcgagcgcta cgccgaccac
     1141 cggcgctgcg ccgtccgagg cgcgcgacga agacgtgctc gagcaggcga gcgcgctcgt
     1201 gcccgcgtca cgccgcgaca agttccaggc gctctatgtc gcgctcagcg gctccacatt
     1261 tgggccggcg ggtcgcacgg cgtcgccggc tgcacgtgcg gcgcgtgcac tcgcgcttgc
     1321 gggtcgcggc gaagacacga tcagcgcgcg tgaacgtgcg acggtcgcgt gggacgtgct
     1381 gccggtcgtc tatggtgtcg atgctggcga cgagccgctg tcgacgggcg atgcagccgc
     1441 acgtgcagtg cgtcgccgcg acgagatgcg cgcgctcgat ccgatcttcg tcgattcgcg
     1501 gcccggtctc gcgtcgttgt ccgcgcgcgc cggtgaggcg ctcggttcgt atgttgcgtc
     1561 gccggcgcct gcgccgtcgt ccgcatcgtc ctcgtcttcc tcgcgcgagg tcggcgcggt
     1621 gctgcgtccg ccgacggccg cgcccgagtt cgtgcagacc ggccgtccgt cgggtcgtac
     1681 cggtggtggc gaggtcgaga ttccggcgtg gttcgagcag gcggcgcgga agatgttcga
     1741 gtcgaagtcg aatgtctctg acgggatctc gatcgccgag ctcacgctcg tctctaccgc
     1801 gccggctccg caggttgccg cgtcgacgcg cgtctccggt ggtgcgccac cggtcgcggt
     1861 tgcaccctcg gcagcggtcg gtgccgagaa gcaagcgatc gatatcgaga aggtcgcgaa
     1921 tgacgtctac cgcgaagtgc ttgtcctcat ggatcttgcg cgcgcacgta acggggagcc
     1981 ctaccaatga gcaacggcaa caacaagaat gtcgatggca gcacgttcgc tccggtcgga
     2041 ggaggagcga cgagctccaa caatgccaag gcgggcaaga tcatgatcgg cagcttggac
     2101 gacccgagca tctacgtgga ggcgcagtac aaccccaagg agctcgagat cagcaagacc
     2161 gttccctggt cgaaggtgaa cgaagcgaac aaggccaacc aaaaggcgag tcaggggcag
     2221 ggcatccaca tggagttcac cggcgccgag gggcgctcgc ttaccgtcga gatgctgttc
     2281 gactgctacg aggacgacga caagacgaag gtcgcaaagt cgatcgagaa gctcgagaaa
     2341 ctcgcgtcgg tgcgcgtgcc cggctctccc aaagaggaaa acaaacgccc gcatcgctgc
     2401 atggtggtct ggggcgacgt cgtcaagatg agctgcgtga tcgacggtct cggcgtcaag
     2461 tacacgatgt tcagcagcga cgggaagccg ttgcgcgcga cctgcagcgt caagctcaag
     2521 gaagccgatg tcgtgtccgc gaagaaggcc ggaaagtgaa ccatggccga cgggaccgca
     2581 gcgcctaatt acacgatcaa gatcggcacg cgcgacgcga ccgagctgaa gaccgacttt
     2641 ctgtcgttcg tgatcgagcg cgacatgttc cagcccgaca tggcggcgat cgtgctcagc
     2701 aaccaggctg gccagtactc gacgatcaag atcgctgacg aggtcgacat ctgtgtcggt
     2761 gacgacgctg gtacatcgat ctacaagggc gaggtcgttg gcctcgagcc ggtctacaag
     2821 ggtggcgaga agtcacgcat cttgatccgc gcgatgaaca aattccatcg cctgctgcgg
     2881 cactgcaagt cggtgacgtt taccgataag accgaccagc agatcctgaa tcaggttgtc
     2941 ggcgacgcgg ggctctcgct gtcgtggcaa cacgaaaaat cgatcacgta caagcacgtc
     3001 taccagcaca accagacgga catggagttc cttcgcacgc gtgcagcgcg catggggtgc
     3061 catgtctggt gcgtcgacaa aacgatctac gtcaaggaac cgaacctcca gcaggggccc
     3121 gcagtcacgc tgaatatcag cgaaggcggc gggtcgtctg accagccgac gatcaagtcg
     3181 ttcacgccgc ggctctcgtc ggcgagcatc ctcaagaagg taacggtcaa aggctggaac
     3241 ccggaaacga aggagctcat cgtcggcgat tactcggcga aggactcgaa gctcggtgac
     3301 acgaatgcgg tttccgcgtc cgcagagctc ggcaaggaag agacgttcac ggtcgatcat
     3361 ccggtctgga gcaaagagga agcggtcgcg ctcgccaagg cacgcctcgt cgatctctcg
     3421 ctcagcttca tcaccggtga gtgcgaggtg accggaacga ccaagctcga cctcggcacg
     3481 gtgatcggca tccaagcgaa cacagatccc gataccaagg gcgacgatcc gttcaacggg
     3541 aactactacg tcatgggcct cacccatcgc ttcgtgctgt cgaagaccaa ggaaggcggc
     3601 ttcaccacca ttcttcggct cgcgcgtgac gcgcagaaga agggctagct gtgagggcag
     3661 gcggaccgcg gcgagattcg ctcgaccacc tgcagtacgg ggtgcactac ggcatcgtgg
     3721 tccagaacaa ggatcccgag aatctcgatc gcgtgaaggt ccgcttgccg tggctcgatt
     3781 cgggcgacgt cgatcagtcg cactgggcgc agctgctgac gccgatggag ggcaagaagt
     3841 tcggctggta cacgctgccc gatatcgacg acgtcgtcgc ggtgatgttc atcaatggcg
     3901 acacgagcca gccagtgatc gtcggtggca tctggtcgaa gcccgacttc tcgccggaga
     3961 cgaacgaaga cggcaagaac aactttcgcg gctatcgcag ccgcgcgggc catcggctga
     4021 tcctcgacga cacggcgaag acgaaggtcg tgttcgccga caagaccacg aagaacatga
     4081 tcggcatcgg caacttcgcg aaggacggcg ccggcccgaa cgtgtgcgcg gtctacaagc
     4141 cgccgatgtc cggagacgtc ggcgtctcga tctcgtcgat ggaaggcacg atggagatca
     4201 cgtgtaagga cggcgcgctg aaggtcagcg ctggccagga catcaagatc aacgcgaaga
     4261 ccaccgtcga cgtcaaagcg ggtggtgacc tcacggcgga aggcagctcg gcggcgaagc
     4321 tgacgtcgag cgacaactcc aactacgacg gctcgaaggt ggacatcgcg tgacgttgtt
     4381 cccgatcgat cgcccgatgg agacggcgct tcgcgcagcg aagatgcgcg gcgttcatct
     4441 cgcgatcgtc gtcgagaaca aagacggcgg agacaatccc ggctaccgcg tgtgcgtgaa
     4501 gtacccgtgg atgaacgagc aagagaagtc gttctgggcg cgcatcgcga tcccgatggc
     4561 cggcaaagag cgcggcacgt acttcttgcc ggaagtcgat gaccagctac tcgtcgtgtt
     4621 cgagcacggc gacatcaaca agccgatcgt gatcggcgcg ctgtggtcga agaagcaaga
     4681 gccggtcgag atcaacgaga gcggcaagaa caacacgaag ctgatcaagt cacgcgccgg
     4741 tcaccgcatc atcttcgatg acaaagacgg cgccgagaag atcacgatcg tcgacaagac
     4801 aaagaagaac aagatcgttc tcgactcggc caacaaggtc gtcaagatcg agagcgcagg
     4861 tgacatcgag gtcaaagcag cggccaacgt catcatgcac agcaacgcgc tgaaggtcgg
     4921 cacgtccgac acgttcaccg cgaagggaca gcaggtgctg acgcactcgg gcgccgcgtt
     4981 tggcgtcaag gcgagcagca acatcacggt caacggcgcg caggtgcaga tcaacgtcgt
     5041 caattcgccg gcggcgcgcg tgtcgggctc gggcgccggc gagctcggag gcgctgcggc
     5101 cgaaacgccg aaggaccagg tccaagagca aacgcgtggc ggtggaggcg gcggcggtgg
     5161 tggcggtgct ggtggcggtg gtggcggtgg accggcgccg gcagcaccgg agccggctcc
     5221 gagcagctcg tcgagcggcg gtggatcgtc gtcgggggga agcggatcgt cgagcgcggg
     5281 cacggcaggc agtcctggcg gcgcgtcggg acagccgggc ggtgggcaag gcggcgcgag
     5341 tggcagcgca agcgcaagcg cgacggcgag tggcggcgcg agtggatcag cgcaaggcgg
     5401 tgcggcaggc ggagcacaag gcggcgcgag tggcagcgca agcgccggtg cgaccgcgag
     5461 cggcggcgcg agtggatctg cacaaggcgg cgccggtggc ggagcacaag gcggcgcgag
     5521 tggcagcgca agcgcaggtg cgacggcgag cggcggcgcg agcggatctg cgcaaggcgg
     5581 cgcgggcggc ggagcacaag ctggcgcgag cggtagcgct ggcgctggtg caacggcgag
     5641 cggcggcgcg agtggatctg cacaaggcgg cgcgggcgca caaggcggag cacaagctg
//
LOCUS       DASUFX010000789         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_126156,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000789 DASUFX010000000
VERSION     DASUFX010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126156"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFV99_31785"
     CDS             <1..650
                     /locus_tag="VFV99_31785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064019.1"
                     /translation="ETDLVTVLHADDELMPGYCALMRGASLRHPQAAAFYCRTEVIDA
                     DGNRTFSLLDAVKDRLINPSVGKETLLSGEPGFYALLHGNFINAPTLCFRKSVLGNRR
                     FPNHKFILDWELTSQLVLDGDTIVGLPERCYRYRRHPEAATSQYTKSQLRFREESEFF
                     DLMIALAQRRGWTRSERFARKKRIVKLNLLYFALRSAARGDLAEARRDIQMLREL"
     gene            complement(645..1625)
                     /locus_tag="VFV99_31790"
     CDS             complement(645..1625)
                     /locus_tag="VFV99_31790"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5064020.1"
                     /translation="MSLVEGLEIAVIVPCFNEAAAIDKVVRDFRASLPTATVYVYDNN
                     STDNTAEVARAAGAEVRRETRRGKGNVVRRMFQDIEADVYVMVDGDDTYDASVAPKLV
                     DKLVDENLDMLVGRRVETHQAAYRMGHRLGNRVLTGLVAWLFGSQIDDMLSGYRVFSR
                     RFVKSFPASSREFEIETELTVHALQMKMAIAEVETNYKERPPGSVSKLRTFRDGWRIL
                     VTIINLMRNERPLLFFSLLSLVFLAVGIILATPVFLEWLDTGVVRRFPTAFLSFTLGV
                     IAVVCWATGLILDLVAHVRREAKRLAYLQLAAPREATKTAVSGARKQKAS"
     gene            complement(1573..2406)
                     /locus_tag="VFV99_31795"
     CDS             complement(1573..2406)
                     /locus_tag="VFV99_31795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064021.1"
                     /translation="MGTQANERGQVFARQVVPATATTRAESRTIYLNPNGVVLRPGDN
                     DSTHDISSIIDQPTAINGWDIDAETWQDTVDCMREIYAPFDVTITEDDPGDVPHIEAV
                     FGGSPLDLELPANVAGVSPFTLDCAIIERSIVFTFTEVLDDDPRTVCEVMAQEVAHSF
                     GLDHELLASDPMTYLTYFGERTFQNEMASCGEYGGRMCGINGSICRTEQNSFALLSDR
                     LGPSPSARPAEDEENAGEGGIRGGCSAGGGGVGIGFVLLVGHLVARRRTRDRRHRAVL
                     Q"
     gene            2679..4061
                     /locus_tag="VFV99_31800"
     CDS             2679..4061
                     /locus_tag="VFV99_31800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064022.1"
                     /translation="MNAETPARNTGWWRALSDAPPAAILAVGWLVLVVYAYPGQLSWD
                     SLEYLRAARDGFYTDAHPPAMSGLWSLTELFVAGPSGMFLLQSLAFITGLYLAMRKTF
                     PARGAAWSAALLTVFPPILLPMGVVDEYALMVGLLMLGFGALLSIQRRINVLGLVALG
                     FAIAVHPTARVAAFPLVVLLFEWPSRPALRRYTVAVGVWLAMTVLAAGANWALTDLQI
                     GHARAERNLFDIAGVLAYSDADRADADAQRVLAGTGLRIDHMIHAKVRASYAPRDAST
                     LLSGDNAIWELPLAPDTPVPDAQRDAIARAWDNLALGRPLQYLSHRLAVTECVLGILD
                     LEHQAVVVPRYPVAREIMLDAGLATRSSGLQDKMTDAARALVDNTPLFVPWIYLVLGL
                     VLLPFAGRYRDIVALLVSGLAAEATLFVTAVDNSYAQSLWLITCACIALVAVIARKRT
                     WKRRKTTVHT"
     gene            4058..5365
                     /locus_tag="VFV99_31805"
     CDS             4058..5365
                     /locus_tag="VFV99_31805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064023.1"
                     /translation="MTALLADIRRWFRGLSPFAILLIGWLTFMLYAYPGFMSFESVDQ
                     LLQARASEISAAYPPLMTALWQVVDCVFAGPFGMLVIQTVCFLAGAYLLLKRFMPARP
                     AAFCASLLLVVPPVAAVMAVIWSDSQMTGFLLLGTALVLSPRGSVKIGSLALFIAATA
                     MRIDAAALTFSLIVLLFAWTPTLRWYKRYPIALLVWVAVTLAAIVVNNALTTADENSH
                     PWHDTIAPSDIAGTLRNAPPLSDAELQPILANTPLLPTHDIQDAAQTELFYDMPRTAA
                     ERAAIGRAWKELIPTYPAAYLEYRLQLLIDRIRLHGDDIASTTYVWFTDVNDPDSAAK
                     LGFSARPAGIQTVLRDGMRAAGSTELFRPYVYLLLALVLLPFCFRDRELLALGLSGLV
                     GELALFFTATSLDYRYSIWLVVTAIMVAMMLAKRNRTRRDSTG"
BASE COUNT          888 a         1919 c         1759 g         1053 t
ORIGIN      
        1 ccgagaccga tctcgtcacc gtgctgcacg ccgacgacga gctgatgcca ggctactgcg
       61 cgctcatgcg cggcgcctcg ctccgtcatc cgcaggcggc cgcgttctac tgccgcactg
      121 aagtgatcga tgccgacggc aatcgcacgt tctcgctgct cgacgccgtc aaggatcgcc
      181 tgatcaatcc gagcgtcggc aaggagacgc tgctctccgg cgagccgggc ttctacgcgc
      241 tgctccacgg caacttcatc aacgcgccga cgctgtgctt ccgcaagagc gtcctcggta
      301 accgccggtt cccgaaccac aagttcatcc tcgactggga gctgacgtcg cagctcgtcc
      361 tcgacggtga cacgatcgtc ggcttacccg agcgctgcta tcgctatcgc cgccaccccg
      421 aagccgcgac gtcgcagtac acgaagtcgc agctgcggtt tcgcgaagag tccgagttct
      481 tcgacctcat gatcgccctt gcccagcgcc gcggctggac acgctccgag cgcttcgcgc
      541 gcaagaagcg gatcgtgaag ctcaacctcc tctacttcgc gctgcgcagc gccgcccgtg
      601 gcgatctcgc cgaggcgcgt cgcgacattc aaatgctgcg cgagctatga cgccttctgc
      661 ttgcgcgcgc cgctgaccgc cgtcttcgtc gcttcgcgtg gtgccgcgag ctgcagatag
      721 gcgagtcgtt tcgcttcgcg ccgcacgtgc gcgacgagat cgaggatgag acccgtcgcc
      781 cagcacacga ccgcgatcac accgagcgtg aagctgagga acgcggtcgg gaagcgccgg
      841 accacgccgg tatcgagcca ctcgaggaat accggcgtcg cgagaatgat accgactgcg
      901 aggaacacga ggctgagcag cgagaagaac agcagcgggc gctcgttgcg catcaggttg
      961 atgatcgtca cgaggatgcg ccagccgtcg cggaacgtgc gcagcttgct caccgagcct
     1021 ggtggccgct ccttgtagtt cgtctcgacc tcggcgatcg ccatcttcat ttgcagcgcg
     1081 tggacggtga gctcggtctc gatctcgaac tcgcgggacg acgccgggaa gctcttcacg
     1141 aaccggcgcg agaacacgcg atagcccgac agcatgtcgt cgatctggga accgaacagc
     1201 catgcgacga ggccggtgag cacgcggtta ccgagccgat gccccatgcg atacgcggcc
     1261 tggtgcgtct cgacgcggcg tccgacgagc atatcgaggt tctcgtcgac gagcttgtcg
     1321 accagcttgg gcgcgaccga cgcgtcgtac gtgtcgtcgc catcgaccat cacataaacg
     1381 tccgcctcga tgtcctgaaa catccggcgt acgacattgc ccttgcctcg gcgggtttct
     1441 cgtcgcactt cagcgccggc ggcacgcgcg acttccgccg tgttgtccgt cgagttgttg
     1501 tcgtagacgt agacggtcgc ggtgggcaag ctcgcgcgga aatcgcgaac caccttatcg
     1561 atcgcagctg cctcattgaa gcacggcacg atgacggcga tctcgagtcc ttcgacgagc
     1621 gacaaggtgt cccactaata acacgaagcc gatcccgaca cctcccccgc ctgccgagca
     1681 gccaccacgg atcccgcctt ccccagcgtt ctcctcgtcc tcggcgggcc gtgcgcttgg
     1741 actgggcccg aggcggtcgc tcagcaacgc gaacgagttc tgctcggtgc ggcagatcga
     1801 gccattgatg ccgcacatgc ggccgccgta ttcgccgcac gatgccatct cgttctggaa
     1861 cgtgcgttcg ccgaaatacg tcaggtacgt catcggatcg gatgcgagta gctcgtgatc
     1921 caacccgaag ctgtgagcca cttcctgcgc catcacttcg cacacggtgc gcggatcgtc
     1981 gtcgagcacc tcggtgaacg tgaacacgat cgagcgctcg atgatcgcgc agtcgagcgt
     2041 gaacggcgat acgccagcga cattcgcagg caactcgagg tcgagcggcg agccgccgaa
     2101 cacggcctcg atgtgtggca cgtcacccgg atcgtcctcc gtgatcgtga cgtcaaacgg
     2161 cgcgtagatc tcgcgcatgc aatcgaccgt gtcctgccag gtctccgcgt cgatgtccca
     2221 accgttgatg gcggtgggct ggtcgatgat cgacgaaatg tcatgcgtcg aatcgttgtc
     2281 gccgggcctg agcacgacgc cgttcgggtt gagatagatg gtgcgactct cggcccgcgt
     2341 ggtcgccgtc gccggcacga cctggcgcgc gaacacctgg ccacgctcgt ttgcctgggt
     2401 tcccaccagc tcgaacatgc gagcgctgcc gctttcgcgt agcggacgtg cgctggctac
     2461 cgcgggtaac aacgacacta gaacgagcaa cctgcatccc ataggtggca cgccgataca
     2521 tcacgcccca tgccagcaga tcgtgcgcgg gcactgcgac ttatcgtgat ggcgtctgga
     2581 gccttcagga cgtggtgcgg gggagtgtcc tctccgtcga ctccagtaaa cgtcgaccgc
     2641 cgttgacgat cgttggcgtg atactagaga cagagtcgat gaacgccgag acccccgcgc
     2701 gcaacaccgg ttggtggcgt gcgctctccg acgcgccgcc cgccgcgatc ctcgcggttg
     2761 gctggctcgt gctcgtggtc tacgcgtacc cgggccagct gagctgggac tccctcgagt
     2821 acctgcgcgc cgcgcgagac gggttctaca cggacgcgca tccgccggcg atgagcggcc
     2881 tgtggtcgct caccgagctc ttcgtcgcgg ggccctcggg aatgttcttg ctccagagcc
     2941 tcgcgttcat caccgggctc tatctcgcga tgcgcaagac gttcccggcc cgcggcgcgg
     3001 cgtggtcggc ggcgctgctc acggtgttcc cgccgatcct gctgccgatg ggtgtggtcg
     3061 acgagtacgc gctcatggtc ggcctgttga tgctcggttt cggcgcactg ctttcgatcc
     3121 agcgccgcat caacgtgctc ggcctcgtcg cgctcggctt tgcgatcgcc gtccacccga
     3181 ccgcgcgcgt cgccgcattt ccgctcgtcg tcctcttgtt cgagtggccg tcgagacccg
     3241 cactgcgccg ctacacggtt gcggtcggcg tgtggctcgc gatgaccgtg ctcgctgcag
     3301 gcgcgaactg ggcgctcact gatctccaga tcggccacgc acgcgcagag cgcaacttgt
     3361 tcgacatcgc tggcgtgctc gcctactcgg acgccgatcg tgcggacgcc gatgcccagc
     3421 gcgtgctcgc cggcaccgga ctccgtatcg atcacatgat ccatgcgaag gtccgggcga
     3481 gctatgcccc gcgcgacgcg tcgacgctgc ttagcggcga caatgcgatc tgggagctgc
     3541 cgctcgcccc cgacacccca gttccagacg cgcaacgcga cgcgatcgcg agagcatggg
     3601 acaacctcgc cctcgggcgt cctctccaat atctcagtca tcgcctggcg gtgaccgagt
     3661 gcgtgctcgg catcctcgat ctcgagcacc aagcggtcgt cgtgccacgc tacccggtcg
     3721 cccgcgagat catgctcgac gctgggctag cgactcgctc gtcgggactt caggataaga
     3781 tgaccgacgc cgcccgggcg ctcgtcgaca acacgccact gttcgtgccg tggatctatc
     3841 tcgtgctcgg cctggtgctc ctgccgttcg cggggcgcta ccgcgatatc gttgcgctgc
     3901 tcgtgagcgg cctcgcggcc gaggcgacgc tgtttgtcac agcggtcgac aacagctatg
     3961 cgcaatcgct gtggctgatc acgtgcgcct gcatcgcgct cgtcgcggtg atcgcgcgca
     4021 agcggacctg gaagcggcga aagacgacgg tgcacacgtg acggcgctgc tcgcagacat
     4081 ccggcgctgg ttccgcggcc tctcgccgtt cgcgatcctg ctcatcggct ggttgacgtt
     4141 catgctctac gcctacccgg gcttcatgag ctttgagtcg gtcgatcagt tgctccaggc
     4201 gcgcgcatcc gagatcagcg cggcgtatcc cccgctgatg accgcgctct ggcaggtcgt
     4261 cgattgcgtc ttcgccgggc cgttcggcat gctcgtgatc cagaccgtct gcttcctcgc
     4321 tggggcgtac ctgctgctga agcgcttcat gcctgcgcgt cccgccgcgt tctgcgcgag
     4381 cctcctgctc gtggtgccgc cggtcgctgc ggtgatggcc gtgatctgga gcgactcgca
     4441 gatgacaggc ttcttgctgc tcggcaccgc gctcgtgctg tcaccgcgcg gttcggtcaa
     4501 gatcggcagc ttggcgctgt tcatcgccgc aactgcgatg cgcatcgatg ctgctgcgct
     4561 gacgttctcg ctgatcgtcc tcttgttcgc atggacgccg acgctgcgct ggtacaagcg
     4621 ctacccgatc gcgctcctcg tgtgggtcgc cgtcacgctc gcggcgatcg tcgtcaacaa
     4681 cgcgctcacc accgccgacg agaacagcca cccgtggcac gacacgatcg cgccgagcga
     4741 catcgccggc acactccgca atgcgccacc gctctccgac gccgagctac agccgatcct
     4801 cgccaacaca cccctcttgc ccacgcacga catccaggat gccgcgcaaa ccgagctgtt
     4861 ctacgacatg ccgcgcacgg cggcggagcg cgcggcgatc ggacgcgcgt ggaaggagct
     4921 gatcccgacc tatccggccg cctacctcga gtaccgcttg cagctgttga tcgatcgaat
     4981 ccggctgcac ggcgacgaca tcgcatcgac gacgtatgtc tggttcaccg acgtcaacga
     5041 tccagatagc gcggcgaagc tcggcttctc ggcgcggccg gctgggatcc agaccgtgct
     5101 gcgcgacgga atgcgcgccg caggctcgac agagctgttc cgcccgtacg tgtatctctt
     5161 gctcgcgctc gtgctgctgc cgttctgctt ccgcgatcgc gagctcctcg cgctggggct
     5221 atcggggctt gtcggcgagc tcgcgttgtt cttcaccgcg acatcgctcg actaccgcta
     5281 ttcgatctgg ctggtcgtga ccgcgatcat ggtggcgatg atgctggcaa agcggaatcg
     5341 cacgcggcgt gatagcactg ggtgatttca ctcggtcgtc ggccgagaat tgtgttagct
     5401 gggagtgcac gtggaagcgc cagcgcctaa aggtcgagta cccgggtccc tccggcgact
     5461 cgcgccttgg ctcgtcgggc tcgcgattct tatcgtcgtg atcacgcgcg tgccgttcga
     5521 cgcgttcggg gatgcgatcg agcatggccc gcacgtgaag ctcgcgatca tcgagctgtt
     5581 gatcacgttc tcggtgctgt gcaccgattc gatcgcaac
//
LOCUS       DASUFX010000790        29599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_126333,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000790 DASUFX010000000
VERSION     DASUFX010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 29599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29599
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126333"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFV99_31810"
     CDS             <1..630
                     /locus_tag="VFV99_31810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064024.1"
                     /translation="IFKHSSRVLQVALPDVYVQPRRPGRLLLANCIEKGRLAPAVIVG
                     RDLMTGYRDTEIAASVGSMIALLRPTYYLKLTLTSADELEAALTAAALIVGQKRTYRP
                     QLEPMIAAIAAELPKRLTRATGEALMQLVHRLAEQPDLARWRNAVDVASQRAALLISG
                     DLAATARMISSEAAAPGGTRPNQRVQELVAYSVSPAYFEARRHLGVNVE"
     gene            complement(688..2223)
                     /locus_tag="VFV99_31815"
     CDS             complement(688..2223)
                     /locus_tag="VFV99_31815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5064025.1"
                     /translation="MRIRRTVSAWVVGWMLMAIFGGAAYAEAAAPAADAPTVEQRLAN
                     LEAYVTNSAPDANGGALAATSGPGHNGWMMISTALVLFMTLPGLALFYGGLVRRKNVL
                     SVLAQCFGCSALVTLIWWAIGYSLVFSKGNAVLGGTGMALFNGVTAAPNGDYAAWVSQ
                     NIFAMFQMMFAIITPALIVGAIAERMKYSAVMAFVALWMFVIYIPLAHMVWGVDGLMN
                     GVWNAAAKIKAIDFAGGTVVHMSSGWSALVLCLILGKRTGFGKRAFMPHSLVLTVVGT
                     GMLWVGWYGFNAGSAVAADGIAANAFVSTTLAAAIAGGVWPALEWVTRGKPTVLGFCS
                     GVVAGLVVITPAAGFVSASSAILIGIIAGVVPYLAVTKLKAIFKYDDALDTFGVHGVG
                     GTIGAILAGVFASPDINGNLNTNLASLLHNGLWLEQLKAVGITMGLSIGGTAAIAYLV
                     KFAIGLRPSAEEEESGLDEIDHGEAGYHEADAIGHGGLPHVALPSIDVSTAPAAAMEA
                     EAS"
     gene            2417..3286
                     /locus_tag="VFV99_31820"
     CDS             2417..3286
                     /locus_tag="VFV99_31820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064026.1"
                     /translation="MIASIALAQPAFADDAVPKPTAAPTELAAAPDPAEATAGPVIST
                     TTDTGAPKVEEPPASRFPRSVIARPLTLPKGLAMLGADATANHDFSAMGGAPIVGYGF
                     TDDFEVQVPYSFATKEFEAKGSVAVDAGYKLLRGAADGKLEAIARVRGGYNALDSVAT
                     PLLAGVHVQYNFTPTIAIISGYAGTEQIRVSLSEDAMMAKPIDISLPIALGYQPTPEL
                     YLQLDTKLAQINLHDSANAFIGADTTPAALTVVYNVINALDVQAAIATDLSNSPGDAL
                     SFLVGARYYAGQL"
     gene            complement(3392..4669)
                     /locus_tag="VFV99_31825"
     CDS             complement(3392..4669)
                     /locus_tag="VFV99_31825"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5064027.1"
                     /translation="MLALRFVGRGLWVTVILLLHGLWWLFGWLGLLVSFRGQAARQDW
                     FAKRLASLLIALGATFVKVGQIMSTRPDLFPPHIIRALTRLQDNVGAFSQRNVERTIA
                     EDLGKPISELFASFDPAPIASASVAQVHRATLPSGAEVAVKVRRPGLDDIVRFDLTVM
                     RMFAHVIAVVPSLRLLAPVESVTEFGRAIRAQIDLRIEAANNKRFTEMFAGDPDITFP
                     PLHEALCSRRVLTMGFVHGVKVLDAPAKQGTDATRLARVGFRTLLKMVFADGFVHADL
                     HPGNILVDERDRVVILDLGLTAELDEAARRAFAQFFAGWAGGNGKIMAKLMADLSPSA
                     HVPDYAAYEREVDEFVGRYLGKALGEVQVSTVAFDMFNILRRHRVRVNPVYTMCNVAI
                     AVTEGIGKQLDPRLDLVQEALPFFATLQTAGRL"
     gene            4701..5483
                     /locus_tag="VFV99_31830"
     CDS             4701..5483
                     /locus_tag="VFV99_31830"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5064028.1"
                     /translation="MASLYDVTTPTGDGTLLVREGWRQGRGAYGGLTIAAAIRAIEQR
                     VADPRRKVRTVTAEIPGPTLPGEAAFSVDVLRSGNSVTVARAALSQAGEVTTHAVAVL
                     AASRPVPDPPTWRDVARPASPPWQDIDPIPRGGPFPEFAQHFEYRIVSGAAGSGGTAQ
                     TIGWVRPREPGASRDAAYIAALIDAWWPAMLVRTGAMRPLGTIAYTLELVDDLVETSD
                     APLLYRGTVPIAGDGYFLETRELWTDDGRLLALNHQTFVVIR"
     gene            complement(5487..6293)
                     /locus_tag="VFV99_31835"
     CDS             complement(5487..6293)
                     /locus_tag="VFV99_31835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064029.1"
                     /translation="MRFAFVLLAACSGSGGGGGGDDVGTDAMPQTSHSGYLQIQSYDA
                     ENTPGTPTRGGTAGAGFWASGAYCTQTKRIDPCTVYTCVTMQPATISGGTVTIAGAAQ
                     AITLMPNALGQYETFMVANPLFNGGETITFTTTGAMVPALTKSIKAPAKATITAPAKP
                     PMASPYLVVNRGQDLTVSWSGGGSGKVQVALNSSMADQRIFCRFDASAGTGKLPSAAL
                     QTFAVGGGGFAMASITEEQAIAGDWAVDVSAYFNAVWPDNSIVSGPTMFQ"
     gene            6346..7758
                     /locus_tag="VFV99_31840"
     CDS             6346..7758
                     /locus_tag="VFV99_31840"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5064030.1"
                     /translation="MRSGWLAVIWLVGGCRSGFDDLSDARGRGDANDARGDTGTVLDD
                     ASDGSTDGSVPLTCTGRTPITGQTWWVATTGNDSNAGTQASPVATITKAVTLASATGG
                     TVVVTPGAYTAEPGGELTIMTTKPLRVVSEQRYRASVPRIVCQTCTDLTIESFEVGGA
                     SLPAISLNGGARVTVLDNVVHDSGSAGIRVTSSADTIAVIGNLVYDNAATDIHFNVTS
                     NITVRGNIVFNATVSPNGLSKIWLEAAATAIVDGNIVFRERGNDNAYGVISLRATSAT
                     TIENNIVAASPNATSVFAPVGFNQASGSATIRHNTFVGPLPGNAFGIGVQSLSSGTYT
                     FVNNVWASSGTAQPFTSGTAPGGTITVRHNLYWNAPNGPFTSGGSPNPTNDSESRVVD
                     PGISFAAVPAAPTWSSSTQQFAGGATTVCDVRTQLVQALAALPATSPAVGGADPAQRP
                     STDVLGKQRPNPATLGAYEP"
     gene            7960..8586
                     /locus_tag="VFV99_31845"
     CDS             7960..8586
                     /locus_tag="VFV99_31845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064031.1"
                     /translation="MHPRKHSQDFLRTGHVLAIGLILLLVGACASTTVDQVWTTPNRA
                     QPPMQKVVTVMISPNTTMRHAAEDRLAIDLANKGVQATPGYQVFPDGAKDVQDLDTMK
                     AHLKELGYDGVVTMRIVDREQVLESAPATFDGYWGYWGPGYWGGYAGGYVYTEIVYRV
                     ESAAYSLHTGQLVWTAMTKTVDPDSAHQLVHDTTKIIASELTKRGLAG"
     gene            complement(8709..9593)
                     /locus_tag="VFV99_31850"
     CDS             complement(8709..9593)
                     /locus_tag="VFV99_31850"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX5064032.1"
                     /translation="MILVHGGAGQVAPDRQERVRAGLRAAAAAGRAILERGGTALDAV
                     VGAVRVLEDDPEFGAGIGSAFTREGTVETCASVMDGSKRRAGAVAAVPNFGMPVVLAR
                     AVLEAGEHVVLAGGAALQYASEIGMQPAAPNGLVTERSAAQFRQAQAHRGAPPGFNEG
                     GGVGAVARDKTGAMAAATSTGGTLYRRAGAIDDSAVPGAGTWADTDCAISTSGGEALF
                     RVALAHNIAMRVADGKSIRAAAKSALQELKKLAPKEAVAGAIMVSKDSWAALNVGPHM
                     PVAWIDDFGPNEAMGFEL"
     gene            complement(9590..10531)
                     /locus_tag="VFV99_31855"
     CDS             complement(9590..10531)
                     /locus_tag="VFV99_31855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5064033.1"
                     /translation="MDLNDILAFTKVVETRSFTGAAEALGLPKSTVSRRLAQLEERLG
                     VRLIQRTTRKVSVTEIGEAYYERCARLVADLALAEQVVTDMQTTPRGRLRVTAPVDFS
                     TACLGAVIAGFVSEYREISIELDATDRVVDLIDEGFDVAVRFSPPSDPALDARRLCAI
                     EGGLVAAPSYLARRGMPTTLDELEAHDRLLFVPSPRFQGWTLTDGSSTYEFGRPARLA
                     SNNFGAVQSAVLAGAGIAAMTDFIVARDIEAGRLVPVMPAWRGRGVAAHLVYPTRAKL
                     PPRLSLFFEHLARSFDPVPWSASARRALVSIADKRSG"
     gene            complement(10535..11254)
                     /locus_tag="VFV99_31860"
     CDS             complement(10535..11254)
                     /locus_tag="VFV99_31860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5064034.1"
                     /translation="MRHGHWLLVVVALAGCEDKKAREAQEKLDRQIERAGGIERDKDG
                     RAYKADTGKYIDSRGGAVSNTERVEGKAITSMSQLPPDPPETDVTRGEQAEFKDDRAG
                     AAKLYKKACAEKVPGGCAHLAMMQREGGGGVKKDPAKAEQELEALCEGGDGYACYGVG
                     IIYETTLTAGKSHSADEVIAMYDKGCTQKSVDACNALGMLYHSGRSGAPKDNAKAAAA
                     LRRGCELGDRMSCLQIDMVKQ"
     gene            complement(11280..12215)
                     /locus_tag="VFV99_31865"
     CDS             complement(11280..12215)
                     /locus_tag="VFV99_31865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064035.1"
                     /translation="MRMLLAATIVLGTSAITYAAPPRVDVVLDECLGPSRDEILRVTR
                     IELGDALSDPAAPGENIHASIACAGPVAVLRVDDPSMQRTLWRRIDLVTGSEPRLLAL
                     ALVELVASLRAELYVPPPSSPPPAAVEHVAPSLATAVAPTSAIPRYRIELTGGAMTFS
                     ADKTALAGLGARLSSRGRMPWLIDVQAHHGSNTTPIGEVTIDVSSLGAALAIERTARS
                     VRASFGAGLRGGVVRMTGTPSSAIVMGSTTLSAWFGPIVLGDIAVPLTGRLALAAAVE
                     VGYVVAPVSAFAGDRRAASVDGAWAQLHVGVGYSL"
     gene            complement(12217..13011)
                     /locus_tag="VFV99_31870"
     CDS             complement(12217..13011)
                     /locus_tag="VFV99_31870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064036.1"
                     /translation="MIDIGREIASTVRADWNDVRREVVWIGLRARRRRRTVMRIACVS
                     AMAAVALVSLREPHDKRAAPLVDVVADESAESLTAIKRAVPVERPPTHEPQPAASSGE
                     PAVPSRRRVIAQRAEFSTWRELARVGEYERAYEVLDGVHENADDLLMIADVMRLSHHP
                     AEATEPLRRVVRDHADDPRASLAAFTLGRVLLDDLGEAREAAGAFADAIRLAPQGPLA
                     EDALARRVEALARAGDTDEAHALAFQYLDQFPRGRWLRMVRRFGGL"
     gene            complement(13008..13520)
                     /locus_tag="VFV99_31875"
     CDS             complement(13008..13520)
                     /locus_tag="VFV99_31875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5064037.1"
                     /translation="MRDDETVDATATDLVSVFRNYSRYVASIALRLLGRDDDVDDVVQ
                     EVFLIAMRGLDSVRDPAAIRGWLATITVRVARRKMRRRRLWAFVGLDAAPDHARLVVD
                     AAQDKAVLIVRAYSALEHMPVDDKIAWMFRYVEGEPLESVAKICGCSLATAKRRIARA
                     QAALALEVDE"
     gene            13724..14629
                     /locus_tag="VFV99_31880"
     CDS             13724..14629
                     /locus_tag="VFV99_31880"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064038.1"
                     /translation="MRRQIAAASLTALTACVGTADSVLEPTPPEPDHTNTLSRDVIGV
                     AAGMSFSCGLNRDGTIACWGYAWDPTEMTPPYGSFTAISAGNSHACALRLDGTITCWG
                     SERETLPETLVAPPGRFISISAGNTHNCALRPDRTAVCWGQNRYGESVAPAGTFLAIS
                     PGFNHTCGVRDNGAIECWGRADMYGTPPAGTYVDVTVRNTGYMNCGLQSSAVPSCWAA
                     PSPIRAPQEPFITISSGFDYTCGIREAGTLICWSHEGEAGWAPAGEYVALQGGYYHFC
                     AIRNDGKVVCWGNDWYGQSSTPPQT"
     gene            15025..16041
                     /locus_tag="VFV99_31885"
     CDS             15025..16041
                     /locus_tag="VFV99_31885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064039.1"
                     /translation="MRVLGVLVLVASCGAPSSSPSTIDAPPTSGSDAASDGSKPDAAV
                     DGTTVVDDPCFPSPHAGHTIYTCMGLAFDVEVPAACIAGGCGIILDVHGLTMSAAMED
                     ANTELRARGGAAGFVVIQPSANPAPPQSSFSPVDDDPKIWDFLTRAVAVYHIDSHRIH
                     VTGFSQGGFMTWRFLCAHADVLASVAPGAAATNCPPFLPTPACSFTGAEIPSQQIPVL
                     YMHGTHDDDYVPYSCAQPQVDAMVAAWGLTSNGVIASSSMYRRSRWTSASGTVVELLS
                     HDYSSEAQVPLVSQTKLLGHCYPGSTDPGNQPGQLFSFKCEQTAAFTWGTEVVAFFQA
                     HPRP"
     gene            16322..17104
                     /locus_tag="VFV99_31890"
     CDS             16322..17104
                     /locus_tag="VFV99_31890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064040.1"
                     /translation="MKRQVLNLRNVLACLVVSAAGPGTALADNNAACPEEEANCPTHA
                     QPVQQPAPAPAPMPAEQPTEEQRVESDLHKYGIAISAGGGVADWFSGGMRDLTGTSGT
                     WDVRLYFGLRSQLGAEVAYVGRASSLDTPFNAAINSTTLYGNGVEVLGRLNVVPDMAV
                     QPFVFLGVGWTRFNTTDTLVLADTGVNDSSDTLAFPLGAGVDWKAPFGLLVDARFAFI
                     PTTNDDFIANGQTVVINNVPVVTGGFLDLDSWNFSANVGYAF"
     gene            18013..21366
                     /locus_tag="VFV99_31895"
     CDS             18013..21366
                     /locus_tag="VFV99_31895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064041.1"
                     /translation="MVRRNSEPYTYDPLSEKIIDTSPAGSLDAEEQLELDEILGWRAN
                     GVMPVTVHREEPTRFVVPADATYEEIAVGIGNDAGVHKTDWFEIVPSSQAPKSPDADH
                     QVVRVRAQSLLSATLVGQVMAQLDKMLQPEIDLFVAKLTPWAISPTVIWELIRRVLVW
                     ADRSDWKDASGRSYFDAFLDRLEAVKMTEPKWYTFGRTSDTRTALEWLLRESGEGYWM
                     LRRVIEMRSSHHVSLAPIYTGGNLAPGSPLGYAFVDEFAATILVQELLLVGPTMAKAL
                     LAARNHSYLGLRVIVPVSPTEFFAYGARLALASEGRLGANSEMLFYTGSKVERYVFNF
                     PGVAVIYPDSFSQTQPVGDDREKAERSLVLFEAISAAQDDPKRLLGLDLDVLAGADIA
                     SRKEIFRIILDKGIDLDGGFGLLARVILTTPTDQFVEFEKQLITPAMIKRLAYTSGSG
                     RALLGRAYTMKALELRPLGAQGLENLPVYHVGQSGSTKWWVGVTGEEVQSEAVAPESW
                     NPEDATRIGHEPAVAGQQAGPIKQTGLRFRETEHFTPWYGFGSYANTTEQSEAYHPSQ
                     LLRIVFKGTPDELVASAFELAMVGPVTSPWREVVLPGLMKVVNLWMIYAGARGFIGGM
                     GAAAAMTRGGLQGAELAAAEEAAAALVAKTVRKLLFDIVVLGGSTAVNAYRDELEKTA
                     VGRAFVTICDIALLGLAARDIYLLFSSGVLTKLVKAAGDAIAELGAAAPKRLLDLYDS
                     WRAFASTMATWIKNGFVKIAKTTTGHTMTVPTDEAGFLTMFRTTRAELAGQRVIGGLV
                     AEGRATQAAQEVLDRLKTLAKDSDEFAQAYSAVARRAARLAPGEVNLFLKRVAAVLEA
                     RKAIASKLGGFLTYAMRYDDALAALAQVEQLASRAGISNDAIITLAKKAAAKKIDIGW
                     FNDLDLDTKRLDFMARDPNTQWDLFKRAAENPFDKKAREHVEKVLRGHAGEMLAEEQL
                     AKLLPGYRILGRQARMPPSVLDFLLGAPGRAGIKPALEVKAWNESIWNDVGSALYKRR
                     VSHQAMTANEQLAIEMFDGLVDQLRNAKTASRTEPFLLISKTASRETVAEMLRFFRTK
                     GLRPTLLRFDEAMLDAIINELSRGLGFHP"
     gene            21459..22157
                     /locus_tag="VFV99_31900"
     CDS             21459..22157
                     /locus_tag="VFV99_31900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064042.1"
                     /translation="MFGASDPEDEVEKTWEVVAGTGKYAAIWDYDPAGDSAYQVAPII
                     SRAVRGTVYVAQLHKHLYGVSAYKGGRALDDVVEAPLRLANRLGVPFPPSPVVSPEAE
                     WSVCIVDGAPPDLVNSALDLTPEDRVVVRVDPHPLGTIIYASDRSPVLGVPSMVKGRG
                     LEHATTYAVERSHGDALTIWVYRKGEIILYFCTDERLGLGPPNADRTWDIKGARDVDA
                     ILAALSIPPALLGW"
     gene            complement(22218..22288)
                     /locus_tag="VFV99_31905"
     tRNA            complement(22218..22288)
                     /locus_tag="VFV99_31905"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22254..22256),aa:Cys,seq:gca)
     gene            complement(22314..22399)
                     /locus_tag="VFV99_31910"
     tRNA            complement(22314..22399)
                     /locus_tag="VFV99_31910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22361..22363),aa:Ser,seq:cga)
     gene            complement(22570..23505)
                     /locus_tag="VFV99_31915"
     CDS             complement(22570..23505)
                     /locus_tag="VFV99_31915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5064043.1"
                     /translation="MSESRQVRALIEQADRARRQHNWDTAIDLLKRALAVDPEHDSAH
                     ASLALALLGARRLAGAVIEVDLALGFGPNNPFCHYAAAAVRRAERKLDDAWRHCLVAM
                     QDDDDDVDTRVLGAGIKALQGDHQAAHTLLSEALAIDADHVDALSQLAQLELNAGNHS
                     EALHRIEQALAADPSNVDAHIIAGYIALARNQVDEAERHARFALNQDAGERDALQLWV
                     AIKARRNVVLGLWWRLNMFVGLRSERTQIGLLIGSFVVVRIAIILLDAYGFEDAEIML
                     RKVWLGFCGYTWFAPEIFNWMLKRELSNVVLRDDY"
     gene            complement(23502..23972)
                     /locus_tag="VFV99_31920"
     CDS             complement(23502..23972)
                     /locus_tag="VFV99_31920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064044.1"
                     /translation="MTYEIADEPNPSAGWENLVFQPTAPLLGMMWCGAWLAWPWYIVN
                     AYAMGSPTLRREAKLCALGFAGTLALAMAVFGLVDLGIIESKLALQFAVLSVVSFKLG
                     LAYTVTKIQSRTFHVYTYYGGTAQKAFYVLVAGSYLRDLVLGVSSHPIWQVVLS"
     gene            complement(23969..25207)
                     /locus_tag="VFV99_31925"
     CDS             complement(23969..25207)
                     /locus_tag="VFV99_31925"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5064045.1"
                     /translation="MDETTIKALLDALTASPENVTLRVTIVKLLLDGGAHTRALELAT
                     GEAPEVLILRARALHALNDQPAALAAYEAAVRTNPTLEDRDLRLSLGTVEKLHEEDGV
                     RLRVLSNDDTDASEVDRLLHPEPTKVTFADVGGLDEIKTQIEKRIILPFQKPALFAKF
                     KKKAGGGILLYGPPGCGKTLLARATAGQCNATFLNVAIEDVLDMWIGESERKLHALFE
                     KARATTPAVLFFDEIEALAGKRQYTREATASKLVSQFLSEMDGFAKNNAGVLILAASN
                     VPWAIDSAFRRPGRFDRVLFVPPPDRAARSSILKILLNGRPVADDIDTDLLATRTSGF
                     SGADLENLVDTAADRAIADSLSKSGEVPINHSHLLAALAEIKPTTSEWLTTARNYARY
                     ANEGGQYDDVLAFLAKHTKK"
     gene            complement(25210..26166)
                     /locus_tag="VFV99_31930"
     CDS             complement(25210..26166)
                     /locus_tag="VFV99_31930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064046.1"
                     /translation="MRRWLLAAAVIGLWVWWRGDAPAAHDGERHAAVLADGFALYDGH
                     RVVELDRKGKQRKQHVLAHDGELRLVGPSNGPAVAWVESKKVRLVRASTGKGSVWGNS
                     VRMLCEGAATNDERFAVAWLEANDTIWFVHGDTRNKRTDEQMADEAVGLANADRKVWC
                     GVASAHDLVALFWRDSDRLFIQTCTRKKCSGLPATVSLDRRETLLGFGCVRNACLLGV
                     RDHNGASRLQFVTESGATKWTKPLPTKLLEVSIVGAGENAFAVGFVGEHETEVGRVDR
                     KGVVTPLWAGPPSKHAPALAWSRDQLLVGHPAGATTLVAFPR"
     gene            complement(26254..26832)
                     /locus_tag="VFV99_31935"
     CDS             complement(26254..26832)
                     /locus_tag="VFV99_31935"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5064047.1"
                     /translation="MSDKNGLPTGKHTLIEEGTEFKGSLSSNCPIVVVGKVEGDVTGP
                     MIHVASSGVVAGKVNVKQLRSEGVLAGEVEAETVQIAGTVRDKTRIRARSLEVTLKTD
                     KGMEVVFGECELAIGDEPDKQSAIAAALAPAESAAPATASEVKASSDTASQEAKPTDD
                     AKPSDEAKASDDAKPGDEPKRRKHSNTQSPPP"
     gene            complement(26829..28043)
                     /locus_tag="VFV99_31940"
     CDS             complement(26829..28043)
                     /locus_tag="VFV99_31940"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX5064048.1"
                     /translation="MERADLRGINLSRTQLAKAGFDRADLDGANLEEADLTGATLRRA
                     TLREAYLVGAKLAGANLETSDLEGANLSRANLAGANLSRANLEGVVLDNADLSGAQLS
                     YAQLATAKLGGAKLDNAAFTHAELDEAYLGGASAQGAHFEFATLVRANLEDAKLQKAS
                     FEDANLRHAILRNVDLVEATLVKADLTGADLTGARLMSADVRFANLGRAQLTAANLTG
                     AKIAGLIGTGVPLGPMEVAWLDVSPSGDASQRVENGVIPSLLSGGALAVPTRTEARNR
                     YFGRGDVLRDAALHFDDGARVEIDSLFQNCTIEIGSGTEVIVGPSGVLADCQIIGAGT
                     VTINGKFFERESPGIVGLRQLSVTSKGALMSSVAHSAERTQFAFESGCQLRVKIVGPV
                     SSTSTRNNKVKR"
     gene            complement(28106..28987)
                     /locus_tag="VFV99_31945"
     CDS             complement(28106..28987)
                     /locus_tag="VFV99_31945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064049.1"
                     /translation="MVAGKSAGVQGVSTAEDLEAVRKTFDEVAVAHVSQVRDVMLELR
                     FGDADPTWLAATKPALSSLRAMAVQIDLADLCKALDEFAATIDKAVANRARISDDDKA
                     VLLQRYGRLIELIPKAFELDAERDRREPIIVEALLLQTEGVERPTIAKLFAVGLNRVD
                     ALTNASAEEVAVVSGIRPAIAEAIVSQFKGYRSRTHAAISVRDPAAELGELAQLVKSL
                     GDQNEDFSRAANAWSEDAVARKRELRKQREQTFQRIKVALARLGARDQLSTLERLPFH
                     ERVEKLALYVSAQSQVR"
     gene            29136..29591
                     /locus_tag="VFV99_31950"
     CDS             29136..29591
                     /locus_tag="VFV99_31950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064050.1"
                     /translation="MDGVVVGVVVAGVLVAGVPTAGVPSAERIGVPCGGVFGLTTLGG
                     NAELGRAAANDDEDVATGGALGARFFVIAGVASGFDVVAEGVIGVRVLLLSGIARGTG
                     CDGSRRTGTGAADGTALFAAGALAVAVGDDGDAAAVTADEGAAGALGSS"
BASE COUNT         5208 a         9810 c         9477 g         5104 t
ORIGIN      
        1 atcttcaagc actcgtcacg cgtgctgcag gtcgcgctcc ccgacgtcta cgtgcagccg
       61 cgccgtccag gtcgcctgct actcgcgaac tgcatcgaga aggggcggct cgcgccggca
      121 gtgatcgtcg gccgcgatct gatgaccggc tatcgcgata ccgagatcgc cgcgtcggtc
      181 ggctcgatga tcgcgctgct ccggccgacc tattatctca agctcaccct caccagcgcc
      241 gacgagctcg aggccgcgct cactgcagct gcgttgatcg tcggtcagaa acgaacgtac
      301 cggccgcagc tcgaaccgat gatcgccgcg atcgcagccg agctgccgaa gcgactcacc
      361 cgggcgaccg gcgaagcgct gatgcagctc gtgcatcgcc tggccgagca accggatctc
      421 gcgcgctggc gcaatgcagt tgatgtcgcc tctcagcgcg ccgcgctgct catttcgggc
      481 gatctcgctg cgaccgcgcg catgatctcg agcgaggcgg ccgcaccggg tggcacccgt
      541 ccgaaccagc gcgtgcagga gcttgtcgcc tattcggttt cgcctgccta cttcgaggcc
      601 cgccgccacc tcggtgttaa cgtcgaatag aaaagggccg cgagtttgcg actcgcggcc
      661 ctgttgagga tcagacagga ttctcgatca gctcgcttcg gcttccatcg ccgcggcagg
      721 cgccgtcgac acgtcgatcg atggcagcgc gacgtgcggt agaccaccgt gaccgatcgc
      781 atccgcctcg tggtagccgg cttcgccgtg gtcgatctcg tcgagacccg actcctcttc
      841 ctcggcgctc gggcgtaggc cgatcgcgaa cttcacgagg taggcgatgg cagccgtacc
      901 gccgatcgac agacccatcg tgatgccgac ggccttgagc tgctcgagcc agagcccgtt
      961 gtggagcaac gaggcgaggt tcgtgttgag gttgccgttg atgtcgggcg acgcgaacac
     1021 gccggcgagg atcgcgccga tcgtgccgcc gacgccgtgc acgccgaatg tgtcgagcgc
     1081 gtcgtcgtac ttgaagatcg ccttgagctt cgtgaccgcg aggtacggca cgacgccggc
     1141 gatgataccg atcagaatcg cgctcgacgc cgagacgaag ccagcggccg gcgtgatcac
     1201 gacgagaccg gcgacaacac cggagcagaa gccgagcacg gtcggcttgc cgcgcgtcac
     1261 ccactcgagc gccggccaca caccaccggc gatcgcggcg gcaagcgtgg tcgagacgaa
     1321 cgcgttcgcg gcgatgccgt cggcggcgac cgcgctacct gcgttgaagc cgtaccagcc
     1381 gacccacagc atgccggtac cgacgaccgt cagcacgagg ctgtgcggca tgaacgcgcg
     1441 cttaccgaag ccggtgcgct taccgaggat caagcacaac acgagggccg accagcccga
     1501 cgacatgtgg acgacggtgc cgcccgcaaa gtcgattgcc ttgatcttcg cagcggcgtt
     1561 ccacacgccg ttcatcagac cgtcgacgcc ccacaccatg tgcgcgagcg ggatgtagat
     1621 gacaaacatc cagagcgcaa cgaaggccat caccgccgag tacttcatgc gctcggcgat
     1681 cgcgccgacg atcagtgccg gcgtgatgat ggcgaacatc atctggaaca ttgcaaagat
     1741 gttctgtgac acccatgcgg cgtagtcgcc gttgggtgcc gctgtgacgc cgttgaacag
     1801 cgccatgccc gtgccgccga gcaccgcgtt gcctttcgag aacacgaggc tgtagccgat
     1861 cgcccaccat ataagggtga cgagcgcgga gcagccgaag cactgggcga gcaccgacag
     1921 cacgttcttg cggcgcacga ggccgccgta gaacagcgcg agccccggca gcgtcatgaa
     1981 cagcacgagc gccgtcgaga tcatcatcca gccgttgtgg ccgggacccg acgttgcagc
     2041 gagcgcgccg ccgttcgcat caggcgcgct gttcgtcaca tacgcctcga ggtttgcgag
     2101 gcgctgctcg acggtgggcg cgtcggcggc gggcgctgcg gcttcggcgt atgccgcacc
     2161 gccaaagatc gccatcaaca tccaaccaac aacccaggct gaaacggttc gtctgatcct
     2221 catgcgtatg acgctacccg ccgtaggttt ccaactcgta acgggctcgc aaaatgtctg
     2281 tgcattcgca tagaacgaac gtcaaagcga aagagatacg gctaagcaac gttcacgaaa
     2341 cccgcaaagt gcagggttgg atgccatgcc ccgatccaac gtctcaccat ccctgctcgc
     2401 tgttctacgc gcgtgtgtga tcgcttcgat cgcgctcgcc caacccgcgt tcgctgacga
     2461 cgcggttccg aaaccgacgg cagcgccgac cgagctcgca gcagctccgg atcccgccga
     2521 agcgaccgcg ggccccgtca tctcgacgac aaccgacacc ggcgcgccga aagtcgagga
     2581 accaccggcg tcgcgttttc cgcgttcggt gatcgcgcgt ccgctgacgc tgccgaaagg
     2641 tctcgcgatg ctcggtgccg atgcgaccgc gaaccatgac ttcagtgcaa tgggcggcgc
     2701 gccgatcgtc ggctacggat tcaccgacga cttcgaagtc caggtgccgt actcgttcgc
     2761 gacgaaggag ttcgaagcga aaggcagcgt cgccgtcgac gccggctaca agctgctgcg
     2821 cggcgcagca gacggcaagc tcgaggcgat cgcgcgcgtg cgcggcggct acaacgcgct
     2881 cgatagcgtc gcgacaccgc tgctcgcggg tgttcacgtc cagtacaact tcacgccgac
     2941 gatcgcgatc atctcgggct acgcgggcac ggaacagatc cgcgtctcgc tctccgagga
     3001 tgcgatgatg gcgaagccga tcgacatcag cctaccgatc gcgctcggct atcagccgac
     3061 gccggagctc tatctccagc tcgacaccaa gctcgcgcag atcaacctgc acgactcggc
     3121 gaatgcgttc atcggcgcgg acacgacgcc ggcggccctc acggtcgtct acaacgtgat
     3181 caacgcgctc gatgttcagg cggcgatcgc gaccgacctg tcgaatagcc ccggcgatgc
     3241 gctgtcgttc ctcgtcggcg ctcgctacta cgcgggccag ctctagagca cgctacatgg
     3301 cgcgcgaggg atggcgcgcg cgattggcac ctcacgagtg atttcgtgca ggacggcggc
     3361 ggcgttcgct cacgccgggg ccatgcgaaa gttaaagccg gccggcggtc tgcaaggtcg
     3421 cgaagaaggg cagcgcttcc tgcacgagat cgaggcgggg atcgagctgc ttgccgatgc
     3481 cctcggtcac cgcgattgcg acgttgcaca tcgtgtagac cgggttcacg cgcacgcgat
     3541 gacgccgcag gatgttgaac atgtcgaacg cgaccgtcga gacctgcacc tcgccgagcg
     3601 ccttgccgag atagcggccg acgaactcgt cgacctcgcg ctcgtacgcg gcgtagtccg
     3661 ggacgtgcgc cgacggcgac agatcggcca tcagcttcgc catgatcttg ccgttaccgc
     3721 cggcccagcc cgcgaagaac tgcgcgaacg cgcgacgtgc ggcctcgtcg agctcggcag
     3781 tgagaccgag gtcgaggatc acgactcggt cgcgctcgtc gacgaggatg ttgccgggat
     3841 ggaggtcggc gtgcacgaaa ccgtcggcga acaccatctt gagcagcgtg cggaagccga
     3901 cgcgtgcgag ccgcgtggcg tcggtgcctt gcttcgccgg cgcatcgagc accttgacgc
     3961 cgtgtacgaa gcccatcgtg agcacgcgcc ggctgcacag cgcttcgtgc agcggtggaa
     4021 acgtgatgtc aggatcgccg gcgaacatct cggtgaatcg cttgttgttc gcggcctcga
     4081 tgcggagatc gatctgcgcg cggatcgcgc gaccgaactc ggtgacgctt tcgaccggcg
     4141 cgagcaggcg tagcgacggt acgaccgcga tcacgtgcgc gaacatccgc atcaccgtga
     4201 ggtcgaagcg cacgatgtcg tcgagaccgg gtcgccgcac cttgaccgcg acctccgcac
     4261 ccgacggcag cgtcgcgcga tgaacctgag cgaccgacgc cgacgcgatc ggcgccggat
     4321 cgaagctcgc gaacagctcg ctgatcggtt tgccgagatc ctcggcgatc gtgcgctcga
     4381 cgttgcgctg gctgaacgcg ccgacgttgt cctgcagtcg cgtcaacgca cggatgatgt
     4441 gcggcgggaa caggtctggc cgcgtcgaca tgatctggcc gaccttgacg aacgtcgcgc
     4501 ccagcgcgat cagcagcgac gcgaggcgct tcgcgaacca gtcctgacgc gctgcttggc
     4561 cgcgaaagct cacgagcagg ccgagccagc cgaacagcca ccacagcccg tgcagcaaca
     4621 ggatgacggt gacccagagg ccgcgaccga caaagcgcag cgcgagcatc gccggacgtt
     4681 atacatgcta catcgctcgg gtggcgagtc tgtatgacgt aacgacaccc accggcgacg
     4741 gcaccttgct cgtgcgcgag ggctggcgcc aaggtcgcgg cgcatacggt ggtctgacga
     4801 tcgccgctgc aatccgcgcg atcgagcaac gcgtcgccga tcctcggcgc aaagtacgta
     4861 cggtcaccgc cgagattccg gggccgacgt taccgggcga agcggcattt tccgtcgacg
     4921 ttttgcgcag cggcaactcg gtcaccgtcg cgcgcgccgc gctctcgcag gccggcgaag
     4981 tcaccacaca tgcggtcgcc gtgctcgccg cgtcgcggcc cgtgcccgat ccgccgacgt
     5041 ggcgcgacgt cgcaaggccg gcgtctccgc cgtggcagga catcgaccca ataccgcgcg
     5101 gcggcccgtt ccccgagttc gcgcagcatt tcgagtaccg catcgtcagc ggtgcagcgg
     5161 gtagcggtgg caccgcgcaa acgatcggct gggtgcggcc gcgcgagccg ggtgcgtccc
     5221 gcgatgcggc gtacatcgct gcgctcatcg atgcctggtg gccggcgatg ctggtgcgca
     5281 caggtgccat gcgaccgctc ggcacgatcg cgtacacact cgagctcgtc gacgatctcg
     5341 tcgagacaag cgatgcgccg ttgctttatc gcggcacggt accgattgca ggcgacggct
     5401 acttcctcga gacacgggag ctgtggaccg acgatggtcg gttgctcgcg ctcaatcatc
     5461 agacgttcgt cgtgatccgc tagcggctac tgaaacatcg tcgggccgct gacgatcgag
     5521 ttgtcgggcc acaccgcgtt gaagtacgcg gatacatcga cggcccagtc gccggcgatc
     5581 gcttgctcct cggtgatcga agccatcgcg aagccgccac caccgaccgc aaatgtctgc
     5641 aacgcagcgc tcggcagctt gccggttccc gcgctcgcgt cgaagcggca gaagatgcgc
     5701 tggtcggcca tcgagctgtt gagcgcgact tgcaccttgc cgctgccacc gccgctccag
     5761 ctgacggtga gatcctgtcc gcgattgacc acgaggtacg gcgacgccat cggcggcttc
     5821 gcgggcgccg tgatcgtcgc tttcgccggt gccttgatcg acttcgtgag cgccggaacc
     5881 atcgcgccgg tggtcgtgaa cgtgatcgtc tcgccgccgt tgaacagcgg gttcgcgacc
     5941 atgaacgtct cgtactggcc cagcgcgttt ggcatcagcg tgatcgcttg cgccgcgcca
     6001 gcgatcgtga cggtgccgcc gctgatcgtt gccggttgca tggtcacgca cgtgtagacg
     6061 gtgcacggat cgatgcgttt cgtctgggtg caataggcgc cgctcgccca gaatcctgcg
     6121 cccgcggttc cgccgcgcgt cggcgtgccc ggtgtgtttt cggcgtcgta gctctggatc
     6181 tggagataac cggaatgcga ggtctgcggc atcgcgtccg ttccgacgtc gtcgccgcca
     6241 cccccaccac cgctgccact gcatgcagca agaagcacaa atgcgaatcg catccggcga
     6301 gaatactcgc gatcaaggcg ggtggttgcg ggataatgct cgacggtgag gagcggatgg
     6361 ctcgcggtga tctggctcgt cggcggctgc cgatcgggtt tcgatgatct gtcggacgcg
     6421 cgaggacgcg gcgacgcgaa cgacgcacgg ggcgacaccg gcactgttct cgatgacgcc
     6481 agcgatggct cgacggatgg cagcgtgccg ctcacgtgca ccggccgcac accgatcacc
     6541 gggcaaacct ggtgggtcgc gacgaccggc aacgacagca acgccggaac acaagcatcg
     6601 ccagtcgcca cgatcacgaa agcggtgaca ctcgcgagcg cgaccggcgg caccgtcgtc
     6661 gtgacgcccg gcgcctacac ggccgagccc ggcggcgagc tcacgatcat gacgacgaag
     6721 ccactgcgcg tcgtctccga acagcggtac cgcgcgtcgg tgccacgcat tgtgtgccag
     6781 acctgcaccg atctcacgat cgagagcttc gaggtcggtg gtgcgagcct gcctgccatc
     6841 tcgctcaacg gcggcgcgcg cgtcacggtg ctcgacaatg tcgtgcacga cagcggctcg
     6901 gccggcattc gcgtgacgag ctcggcggac acgatcgcgg tgatcggcaa cctcgtgtac
     6961 gacaacgcgg ccaccgacat tcacttcaac gtcacctcga acatcacggt gcgcggcaac
     7021 atcgtgttca acgcgacggt ctcaccgaac ggtctctcca agatctggct cgaggccgct
     7081 gctaccgcga tcgtcgacgg caacatcgtg tttcgcgagc gcgggaacga caacgcgtac
     7141 ggtgtgatct cgctgcgtgc gacgagcgcg acaacgatcg agaacaacat tgttgccgcc
     7201 tcgccaaacg cgacgagcgt gttcgcgccc gtcgggttca atcaggcaag cggcagcgcc
     7261 acgatccgcc acaacacgtt cgtcggtccg ctgcctggca acgcgttcgg gatcggcgtg
     7321 cagtcgctct cgtcgggcac gtacacgttc gtcaacaacg tctgggcgag cagcggcacc
     7381 gcgcagccgt tcacgtctgg caccgcgccc ggtgggacca tcacggtgcg ccacaacctg
     7441 tactggaacg cgccgaacgg tccgttcacg tccggcggtt caccgaatcc cacgaacgat
     7501 tctgaatcgc gcgtcgtcga tccgggcatc tcgtttgctg ccgtgccggc ggcaccgacg
     7561 tggtcatcga gcacgcaaca gttcgccggc ggcgcgacga ccgtctgcga tgtccgcacg
     7621 caactcgtgc aagcgctcgc agcgctgccg gccacgagcc ccgcggtcgg cggcgcggat
     7681 ccggcacaac ggccaagcac cgacgtgctc ggcaagcagc gaccgaatcc ggcgacgctc
     7741 ggcgcctacg agccctgact acagctgctt caggtactcg atcagcgcgg tcttgtcgga
     7801 gccgcaacgc gctcgcagcg accggcgatg tgtgcgcgag taaacgcgtg aacccgttcg
     7861 aggcatgcac actgcgcaag ctacgactgc gcaagctcgg aactgtgcaa ggcacggatg
     7921 ctgctctgtt caaagtcata cctctgccca ggatccgaaa tgcaccccag aaaacattcg
     7981 caagacttcc ttagaaccgg ccatgtcctc gcgatcggcc tcatcctcct tcttgtcggc
     8041 gcgtgcgcat ccactaccgt cgatcaggta tggacgactc cgaatcgtgc ccagccgccg
     8101 atgcagaagg tcgtgacggt gatgatctcg ccaaacacga cgatgcggca cgcggcagaa
     8161 gatcggctcg cgatcgatct cgcgaacaaa ggtgtgcaag caacgcccgg ctatcaggtg
     8221 ttcccggacg gcgcaaagga cgtccaggac ctcgacacga tgaaggcgca cttgaaagag
     8281 ctcggctatg acggcgtcgt gacgatgcgc atcgtcgatc gcgagcaagt cctcgagagt
     8341 gccccggcga cattcgatgg ctactggggg tactggggtc cagggtattg gggtggctac
     8401 gcggggggct acgtctacac cgagatcgtc tatcgcgtgg agtcggcagc ctattcgctg
     8461 cacacaggtc agctcgtgtg gacggccatg acgaagacgg tcgatcccga tagcgcgcac
     8521 cagctcgtgc acgacacgac gaagatcatc gcgagcgagc tcacgaagcg cgggctcgcg
     8581 ggttagtggt gccgcggtgc gcagcagcac gtcggtgcgc acacgtcgaa aaatattcgc
     8641 tcagacgttg ccgtagcgcg cctggccaca cagcgacgag ggccaagatg gacatgactg
     8701 acggcgcgct acagctcgaa gcccatcgct tcgttcgggc cgaaatcatc gatccatgcg
     8761 accggcatgt gcgggccgac gttcagcgca gcccagctgt ccttgctgac catgatcgcg
     8821 cccgcaaccg cctctttcgg cgccagcttc ttcagctcct gcagtgccga tttcgcagcg
     8881 gcgcggatcg acttgccatc ggcgacgcgc atcgcgatgt tgtgggcgag cgcgacgcgg
     8941 aacagtgctt caccgccgct cgtcgagatc gcgcagtcgg tgtcggccca ggttccagca
     9001 cccggcacgg ccgaatcgtc gatcgcgccg gcgcgccgat acagcgtgcc gcccgtcgac
     9061 gttgccgccg ccatcgcgcc ggtcttgtcg cgcgcgaccg cacctacgcc accaccttcg
     9121 ttgaagcccg gaggtgcacc acgatgtgcc tgcgcctgac gaaactgcgc cgcggaacgc
     9181 tcggtgacga gaccgttcgg agcggccggc tgcatgccga tctccgacgc gtactgaagc
     9241 gctgcgccac ccgcgagcac gacatgctcg cccgcttcga gcaccgcgcg cgcgagcacg
     9301 accggcatcc cgaagttcgg caccgccgca accgcgcctg cgcgccgttt cgaaccatcc
     9361 atcacggatg cacacgtttc gaccgtgccc tcgcgagtga acgcggagcc gatgcccgct
     9421 ccgaactcgg gatcatcttc gagcacgcga accgcaccga cgaccgcgtc gagtgccgta
     9481 ccaccgcgtt cgagaatggc gcgaccagcc gcggctgccg cacgcaatcc ggcgcgcacg
     9541 cgttcctgcc gatcgggcgc tacctgccca gcaccaccat gtacgaggat catcctgatc
     9601 tcttatcagc gatcgacacc agcgcgcgac gtgctgatgc actccatggc acggggtcga
     9661 acgagcgcgc gagatgctcg aagaacagcg agagccgagg cggcagcttc gcccgcgtcg
     9721 ggtagacgag gtgcgcggcg actccgcgac cgcgccatgc cggcatgacc ggaacgagcc
     9781 gacctgcctc gatgtcccgc gccacgatga agtcggtcat tgcggcgatt cccgctccgg
     9841 cgagcacggc gctctgcact gcaccgaagt tattgctcgc gagccgcgcc gggcgtccga
     9901 actcgtaggt cgagctcccg tcagtcaatg tccagccctg gaaacgtggg ctcggtacga
     9961 acagcaggcg atcgtgagct tcgagctcgt cgagcgtcgt cggcattccg cgccgtgcca
    10021 gatacgatgg cgcggcaacc agaccgccct cgatcgcgca caaccggcgt gcatcgagtg
    10081 ccgggtcgga cggcgggctg aatcgtaccg cgacgtcgaa gccctcgtcg atcagatcga
    10141 caacacggtc ggtcgcgtcg agctcgatgc tgatctcgcg atactcggag acaaacccgg
    10201 cgatgaccgc gccgaggcac gccgtcgaga agtcgacggg tgccgtgacg cgcaaccgac
    10261 cacgcggcgt ggtctgcata tcggtaacga cttgctcggc gagcgcgagg tccgcgacga
    10321 gccgtgcaca gcgctcgtag tacgcctcgc cgatctcggt cactgaaacc ttgcgagtcg
    10381 tccgctggat cagcctgacg ccgagacgtt cctcgagctg agcgagcctg cggctcaccg
    10441 tcgacttggg aaggcctagc gcctcggcag cgccggtgaa gctgcgcgtc tcgacaacct
    10501 tcgtgaacgc aaggatgtcg ttcaggtcca tcacctactg cttcaccata tcgatctgca
    10561 ggcacgacat gcggtcgccg agctcgcatc cgcgccggag tgccgctgcc gctttggcgt
    10621 tatccttcgg cgcgccgctc cggccagagt gatagagcat gccgagtgca ttgcacgcat
    10681 cgacactctt ctgcgtgcag cccttgtcgt acatcgcgat cacttcgtcg gcgctatgcg
    10741 acttgccggc ggtgagcgtc gtctcgtaga tgatgccgac gccgtagcac gcgtaaccgt
    10801 cgccaccctc gcacagcgcc tcgagctcct gctcggcttt cgcgggatcc ttcttcacgc
    10861 cgccgccacc ttcgcgctgc atcatcgcca ggtgtgcgca gccgccggga accttctcgg
    10921 cgcacgcctt cttgtacagc ttcgcggcgc cggccctgtc gtccttgaat tcggcctgct
    10981 cgccgcgcgt gacatcagtc tccggcggat caggtggcag ctgtgacatc gacgtgatcg
    11041 ctttgccctc gacgcgctcg gtgttcgaga ccgcaccgcc gcgactgtcg atgtacttgc
    11101 cggtgtccgc cttgtacgcg cggccatcct tgtcgcgttc gatgccgcca gcgcgctcga
    11161 tctggcgatc gagcttctcc tgcgcttcgc gcgccttctt gtcctcgcat cctgccaacg
    11221 cgacgacgac cagcaaccaa tgcccgtgcc tcatgcggcc ggagcatcgc acgacaacac
    11281 taaagcgagt agccgacgcc gacgtgcaac tgcgcccagg cgccatccac ggacgcggca
    11341 cgccgatcgc cggcgaacgc gctcaccggg gcaacgacat acccgacttc cacggccgcc
    11401 gcgagcgcga gccgaccggt gagcggtacc gcgatgtctc ccaggacgat cgggccgaac
    11461 cacgccgaca gtgtcgtgct gcccatcaca atcgccgagc tcggcgttcc ggtcatccgt
    11521 accacgccgc cgcgcagtcc agctccgaag cttgcacgca cgctccgtgc ggtccgctcg
    11581 atcgccagcg cggcaccgag cgaagagaca tcgatcgtga cctcgccaat tggcgtcgtg
    11641 ttcgaaccgt ggtgggcctg gacgtcgatc agccacggca tacgaccgcg gctgctcaat
    11701 cttgcgccaa gccctgcgag cgccgtcttg tcagccgaga acgtcatcgc accgccggtc
    11761 agctcgatgc ggtacctcgg aatcgcggac gtcggcgcga ccgccgtcgc tagactcgga
    11821 gcaacgtgct ccaccgctgc cggcggcggc gagctcggag gcggaacgta cagctcggcc
    11881 cgcaacgatg cgaccagctc gacgagtgcg agcgcgagca agcggggctc cgagcccgtc
    11941 acgagatcga tacgtcgcca cagcgtacgc tgcatcgagg ggtcgtcgac gcgcagcact
    12001 gccaccggcc cggcgcacgc gatgctggca tggatgttct cgcccggcgc cgctgggtcg
    12061 gagagcgcgt cgccgagctc gatcctcgtc acgcgcagga tctcgtcgcg agacggcccg
    12121 agacactcat cgaggacgac gtcgactctc ggcggcgctg cgtaggtaat agcgctcgtg
    12181 ccgagcacga tcgtagcggc aagcaacatt ctcatgtcag aggcctccga atcggcggac
    12241 catgcgcagc cagcgcccgc gcggaaactg gtcgagatac tggaacgcga gcgcgtgcgc
    12301 ctcgtcggta tctcctgcgc gagccagtgc ctcgacgcga cgtgcgagcg cgtcctcggc
    12361 aagcggcccc tgcggtgcga gccggatggc atcagcgaac gcgccggccg cctcacgagc
    12421 ctcgccgaga tcatcgagga gcacgcggcc gagcgtgaat gctgcgagag atgcgcgcgg
    12481 atcgtctgcg tggtcgcgaa cgacgcggcg tagcggctcc gtggcctccg ccggatgatg
    12541 ggacagcctc atcacatcgg caatcatcag caggtcatcg gcgttctcgt ggacaccatc
    12601 gaggacttca tacgctcgct cgtattcccc gacgcgcgcg agctcgcgcc acgtcgagaa
    12661 ctccgcgcgc tgcgcgatca cgcggcgtct ggacggtacg gccggttcac ccgatgaggc
    12721 tgcgggctgc ggctcgtgcg ttggtggtcg ctcgacaggt acagcgcgct tgatcgctgt
    12781 gagcgactcg gcgctctcgt cagcgacgac atcgacgagc ggtgccgctc tcttgtcgtg
    12841 aggctcgcgc aacgacacga gcgctactgc cgccattgca ctgacgcacg caatgcgcat
    12901 gaccgtgcgc cgacgacgac gcgcacgaag cccgatccac acgacctcgc gccgtacgtc
    12961 gttccaatcg gcacgcacgg tcgaggcgat ctcgcgcccg atgtcgatca ttcgtccacc
    13021 tcgagcgcga gcgccgcttg cgcgcgcgcg atgcggcgct tcgcagtggc gagcgaacat
    13081 ccgcagatct tcgcgaccga ctcgagcggt tccccttcga cgtagcgaaa catccacgcg
    13141 atcttgtcgt cgaccggcat gtgctcgagt gcgctgtaag cgcgcacgat caacacggct
    13201 ttgtcctgcg ccgcatcgac cacgagccgc gcatgatcgg gagccgcgtc gagccccacg
    13261 aatgcccaca gccgccggcg tctcattttg cggcgagcaa cgcgaacggt gatcgtcgcg
    13321 agccagccgc gaatggccgc cggatcgcga acgctgtcga ggccgcgcat cgcgatcaag
    13381 aagacctcct gcacgacgtc atcgacgtcg tcgtcgcgac cgagcaggcg cagcgcgatc
    13441 gaagcgacgt accgcgagta gttgcggaac acgctgacga gatcggttgc ggtcgcgtcg
    13501 actgtttcgt cgtcgcgcac cagtcgcaat gtccgagcgt gccccattcg cagtctcgga
    13561 cttatcagtg ccgcgcacgc ctcgaaacgg ctcacgcatt acatcgcagc atgatcgctt
    13621 ggctgcgctt gcccgcgcgc gtggtgtgca gcgtcgtgcg caaccaacac tctcgatcgc
    13681 gtgagccgtt tcgagcagcc gcgaccactg ataaggacac accatgaggc gacaaatcgc
    13741 tgctgcatcg ttgaccgcgc tcaccgcgtg cgtcggaacc gccgattccg tccttgagcc
    13801 gactccgccg gagcccgatc atacgaatac gctctcgcgc gacgtcatcg gtgtcgctgc
    13861 gggcatgagc ttcagctgcg ggctcaatcg cgacggcacg atcgcgtgct ggggctacgc
    13921 gtgggatccg acggagatga cgccaccata tggcagcttc accgcgatca gcgccggcaa
    13981 ctcgcacgcc tgcgcgctgc gtctcgacgg cacgatcacg tgttggggtt cggagcgcga
    14041 gacgcttccg gaaacgctgg tggcgccacc cgggcgcttc atcagcatct cggccggcaa
    14101 cacgcataac tgcgcgctgc gacccgatcg aaccgccgtc tgctggggcc aaaatcggta
    14161 cggcgagagc gtcgcgcctg cgggcacgtt cctcgcgatc agtccgggct tcaaccacac
    14221 ctgcggcgtt cgtgacaacg gcgcgatcga atgttgggga cgtgccgaca tgtacgggac
    14281 accaccggcg ggcacctatg tcgacgtgac cgtacgcaac acgggctaca tgaactgcgg
    14341 cctgcaatcg tctgcagtgc cgtcgtgctg ggctgccccg tcaccgattc gggcaccgca
    14401 agaaccgttc atcacgatca gctcgggctt tgactacaca tgtggcatcc gcgaggcggg
    14461 aacgctgatc tgctggagcc acgaaggcga ggccggttgg gcacccgcag gcgaatacgt
    14521 cgcgctacaa ggcggctact atcacttctg cgcaattcgc aacgacggca aggtcgtgtg
    14581 ctggggcaac gactggtacg gacagtcatc gacgccgccg cagacctgat cgagcgacta
    14641 gctcgcagag cgacttggtc gccgagtgct cgcaaccgga caacagcacc cgccggtgcc
    14701 agcccaacaa atcgcgcgtc aggtcggcag ctcggtcccg ttctcatgct tgcgtccctt
    14761 catctcgccg cctcctgcct cctgggttcc tttgggatag caggccgatt cgcagtcgcg
    14821 cgatgactga ctcgactcat gcgtcggata cgcggaagac cgcagcgcac gtcgtgcgaa
    14881 ggcgatgaga gctaccggtt cgtacacaag tagcgcagca cgttggccaa ctggcgcagg
    14941 agacgcggtt tgcgttcgca agcgattggg acgcggcgcg attttcactt gcgtgtcgtc
    15001 gacgcgattc gatacccacg agtggtgagg gttctcggag tcctagtgct cgtcgccagt
    15061 tgtggcgcgc cgtcgtcatc gccaagcacg atcgacgcgc cgccgacatc cggcagcgac
    15121 gccgcgagtg acggctcgaa gcccgatgcg gcggtcgatg gcacgacggt ggtcgacgat
    15181 ccgtgcttcc cgtctcctca cgcaggccac acgatctaca cgtgcatggg actggcattt
    15241 gatgtcgaag ttccggcggc gtgcatcgcg ggaggttgcg gcatcatcct cgacgtccac
    15301 ggactgacga tgagcgccgc gatggaagac gcgaacacgg agctgcgcgc gcgaggtggt
    15361 gccgcgggct tcgtggtgat ccagcccagc gcgaatcccg cgccaccgca gtcgtcattc
    15421 agccccgtcg acgacgaccc gaagatctgg gacttcctca cccgagccgt tgccgtctat
    15481 cacatcgact cgcaccgcat tcacgtgacc ggcttctcac aaggcggttt catgacgtgg
    15541 cgattcttgt gcgcacacgc cgatgttctc gcgtccgtgg cgccgggtgc ggcagcgacg
    15601 aactgcccgc cgtttcttcc gacacccgcg tgctccttca ccggcgccga aataccaagc
    15661 cagcaaatcc cggtgctgta catgcacggc acacatgacg acgattacgt cccgtactcg
    15721 tgcgcgcaac cgcaggtgga cgcgatggtc gcggcatggg gactgacgtc aaatggtgtc
    15781 atcgcgtcga gctccatgta tcgccgctcg cgctggacca gcgcgtcggg cacggtcgtc
    15841 gagctgctct cgcacgacta ctcctccgaa gcgcaagtgc cgctcgtgtc gcaaaccaaa
    15901 ctactcggtc attgctatcc aggctcgacc gatccgggga accaacccgg gcagctgttc
    15961 tcgttcaagt gcgagcagac cgcggccttc acatggggca ccgaggtcgt cgcgttcttc
    16021 caggcccacc ctcgtccgta aactgcgccg ttggcgacac ggggcgcatc gatcgcgtat
    16081 tacgcagggc gcctcgacga catccttagc tacgatcggc tgctatcggc gatagagatc
    16141 gcggcgctcg cgaattgaat cggagtgtga cgtcttgcgg attcagtgcg cgcccgcgca
    16201 tgcgtagtcc gcgtcgcgac tgcatgacaa cggactccaa agcggcgatc tgctcgattc
    16261 tcgggcaacg gccgcaggca cggccaatgc tcttcatgcg gccacacaag gagctgcatc
    16321 tatgaagcgt caggtactga acctgcggaa tgtgctggca tgtctcgtgg tctcggccgc
    16381 tggtccaggc accgcgcttg ccgacaacaa cgccgcgtgc ccggaagaag aagcgaactg
    16441 cccaacgcac gcgcagccgg tgcaacagcc cgcgcctgcg cctgccccaa tgccggcgga
    16501 acagccgacc gaggagcaac gggtagagag cgacctgcac aaatacggca tcgccattag
    16561 tgccggtggt ggcgtggcgg actggttctc cggtggaatg cgcgacttga ccgggacctc
    16621 cggcacctgg gacgtgcgtc tctacttcgg gctccgaagc cagctaggcg ccgaggtcgc
    16681 atacgttggt cgtgcgagca gcctggacac gccgttcaac gcggccatca acagtacgac
    16741 gctctacggt aacggtgtcg aagttcttgg tcgtttgaac gtcgtgccgg acatggccgt
    16801 ccagccgttc gtgttcctcg gcgtcggttg gacacgtttc aacacgaccg acacgctggt
    16861 gctcgccgac acgggtgtga acgacagctc tgacacgctc gcgttcccac tcggcgctgg
    16921 cgttgattgg aaggcaccgt ttggcttgct cgtcgatgcg cggttcgcgt tcatcccgac
    16981 gacgaacgat gacttcatcg cgaatggcca gacggtcgtg atcaacaacg tcccggtcgt
    17041 caccggtggc ttccttgact tggacagctg gaacttcagc gccaacgtcg gctacgcgtt
    17101 ctgatcacgg ttactgaggt gcacatcggc cggcgcgacc ggcgataacc ctcccgtgtg
    17161 tgcctcgaag tgagctgctc gcgcccggct gcgtgctacc gcggccgggc cgcgggcggc
    17221 ggtatttgcg tcgcaagcca ccgcacgaca ggatcgaagt gtggtttgtt gggtggtcga
    17281 tctgaggtcg agcttgatgc agagatcgag cttgatgcgg agaccaggcg gcaggaccgc
    17341 aacgatctcc aggtctgtgt agagcgcgtt cgcaggagcc gtgcgcagcg acgagaacat
    17401 ggacatttct gtccacctca gtccgccaag cactcgtaac tcggcgcccc gggcctaggc
    17461 acgttgcttg cctgttggcg cggcgtgggg cggtatctag cgaaaggggt taggcggccc
    17521 gcggctgaca agcgtgccga gccggtgcca ccagcgctgc aaccgtcacg cttttggccg
    17581 ccgccgcaat cccccgccgg caatgcggtc gcggccattc acgcacgcga gatcgcgaaa
    17641 gcaggtgtcg caaacgcaac caggcccttg ccgcacctcg aacaaattca ggcagcgttc
    17701 ggcaatcaca acgtacgcgg agttcgcgcg acggttggcg gcaatgccgc tgcagcagcg
    17761 agtcacctgg gaggcgaagc gtacgcgatc aatgaccgga tcgcatttcg acgagaaccg
    17821 gatctatggc tcgcggcgca cgaggctgcg cacgtgttcc agcgaggggg aagtgttcgg
    17881 ctccgggtaa gcgatgccgg tggcacggac cgagatgaac agcgagccga ttcgatcgcg
    17941 gacgctgtgg ttgcaggcga atccgccgaa ttgctgctcg acctgcccca acccgatacg
    18001 ggtccgagcg atgtggtccg acgcaattcg gaaccgtaca cgtacgatcc gctgagcgag
    18061 aagatcatcg acacatcacc ggcaggttct ctcgacgcgg aggagcaact cgagctcgac
    18121 gagatattgg ggtggcgggc caatggagtg atgccggtta ccgtgcatcg cgaggaaccg
    18181 acgcgattcg tcgtacccgc tgatgcaaca tacgaagaga tcgcggtcgg aatcggcaac
    18241 gacgcaggcg ttcacaagac cgactggttc gagatcgtcc catcatcaca agcgccgaaa
    18301 tcacccgacg cggatcacca ggtcgtgcgc gtacgagctc aatcgctgtt gtctgcaaca
    18361 ctcgtcggac aggtgatggc tcagctcgac aagatgctgc agcccgagat cgacctcttt
    18421 gtcgcgaagc taacgccgtg ggcgatcagt ccgaccgtga tctgggagct catcaggcgc
    18481 gtgctcgtct gggctgaccg ctccgactgg aaggacgcct ccggccgttc gtatttcgac
    18541 gcgttcctcg accggctgga agcggtcaag atgacggagc cgaagtggta cacgttcgga
    18601 aggacatccg acacacgcac tgctctcgaa tggctcctgc gcgagtcagg cgaagggtac
    18661 tggatgctcc ggcgcgtgat cgagatgcgc tcgtcgcatc acgtctcact cgctcccatc
    18721 tataccggcg gcaacctcgc ccccggttcg ccactcggct acgcgttcgt cgacgagttc
    18781 gccgccacga ttctcgtcca ggagctcctg ctcgtcggcc cgacgatggc gaaggcgtta
    18841 ctcgccgccc gtaatcattc gtatctgggg ttgcgtgtga tcgtgccggt ctcgccgacc
    18901 gagttcttcg cctacggagc taggctcgcg cttgccagcg aaggtcgact cggcgccaac
    18961 agcgagatgc tgttctacac gggctcgaag gtcgagcgat acgtcttcaa tttcccgggc
    19021 gtcgctgtga tctatcccga ttcctttagc cagacgcagc ccgtcggtga cgatcgagaa
    19081 aaagcggagc gcagcctcgt tctgttcgaa gcgatcagtg cggcacagga cgaccccaag
    19141 cggctcctcg ggcttgacct cgacgtcctc gccggagccg acatcgcctc gcgcaaggaa
    19201 atcttccgca tcatcctgga caagggcatc gatctcgacg gcggctttgg tttgctcgcg
    19261 cgcgtcatct taacgacgcc caccgaccag ttcgtcgagt tcgagaagca gctgatcacg
    19321 ccggcgatga tcaaacggct ggcgtacacg tcgggctcgg gtcgcgcgct gctcgggcgt
    19381 gcgtacacga tgaaagctct cgagctccgt ccgctcggtg cccagggact ggagaacctg
    19441 cccgtctatc acgtgggtca gtccggatcg acgaaatggt gggtcggagt tacaggcgag
    19501 gaagtgcaat ccgaggctgt tgcgcccgag agctggaatc ccgaagacgc aacgcgtatc
    19561 ggccacgagc cggcagtcgc agggcaacaa gcaggtccga tcaaacaaac cggcttgcgg
    19621 tttcgcgaga ccgagcactt caccccttgg tatggcttcg gctcgtacgc gaataccaca
    19681 gagcagtcgg aggcgtacca cccatcgcag ctgttacgga ttgttttcaa gggaacgccc
    19741 gacgagctcg tcgcgagtgc cttcgagctg gcgatggtcg gtccggtgac gagcccgtgg
    19801 cgcgaggtgg tcctgccggg gctgatgaaa gtcgtcaatc tctggatgat ctacgcgggc
    19861 gctcgcgggt tcatcggagg tatgggcgcc gccgccgcga tgacccgcgg cggtttgcaa
    19921 ggagccgagc tcgccgcggc ggaggaagcg gcagcggcgc tcgtcgccaa gactgttcgc
    19981 aagctgttgt tcgacatcgt cgtgctcggt ggatcgactg cagtcaacgc ctaccgcgac
    20041 gagctcgaga aaactgcagt agggcgcgcg ttcgtgacga tctgcgacat cgcgttgctc
    20101 ggtctcgcgg cgcgcgacat ctatctactg ttctcgtctg gcgtcctcac caagctcgtg
    20161 aaggcagccg gtgacgcgat cgccgagcta ggtgccgcag caccgaaacg actcctcgat
    20221 ctctacgaca gctggcgtgc attcgcgtcg acgatggcga cgtggatcaa gaacggcttc
    20281 gtcaagatcg cgaaaacgac gaccggtcac acgatgaccg tcccgacgga cgaggcgggc
    20341 ttcctgacga tgttccgcac cacgcgcgcg gagctcgcag gtcagcgcgt gatcggcggt
    20401 ctcgttgctg agggccgcgc cacgcaggcc gctcaagaag tcctggatcg cctcaaaaca
    20461 ctggcgaaag acagcgacga attcgcccag gcgtactcgg ccgtggcccg tcgtgcggca
    20521 cgactcgcac caggtgaggt gaacctattc ctgaagcgcg tcgctgccgt gctcgaagcg
    20581 cgcaaggcga tcgcgagcaa gctcggcggg ttcttgacct atgcgatgcg ctacgacgat
    20641 gcactcgcgg cgctcgccca ggttgaacaa ctcgcgtcgc gcgccggcat ttccaatgat
    20701 gcgatcatca cgctggccaa aaaggccgcg gcgaaaaaga tcgacatcgg ctggttcaat
    20761 gatctcgatc tcgacacgaa acgactcgac ttcatggctc gagatcccaa cacgcaatgg
    20821 gatctgttca aacgagccgc cgaaaacccg ttcgataaga aggcacgcga gcatgtcgag
    20881 aaggtgttgc ggggtcacgc aggcgagatg ctcgccgagg agcaactggc aaagctcctg
    20941 cctggatacc gcatcctcgg acgacaagcc cgtatgccgc cgagcgtgct ggatttcctt
    21001 ttgggtgcac ccggccgtgc cggaataaag cctgcgctcg aagtcaaggc gtggaacgag
    21061 tcgatctgga acgacgtcgg cagtgcactt tataagcgac gtgtctcgca ccaggcgatg
    21121 acggcgaacg aacagctagc aatcgagatg ttcgacggtc tcgtcgatca gcttcgcaat
    21181 gcgaagactg catcacgtac cgagccgttc ctcctgattt cgaagaccgc gtcacgcgag
    21241 acggtcgccg agatgctacg gttcttccgc acaaaaggcc tgcgtccaac gctgctgcga
    21301 ttcgacgaag ccatgctgga cgcgatcatc aacgagctgt cgagagggct cgggttccac
    21361 ccctgatgat gccaacgttc cgttatccga cctgcgcgat catcgttcca acgacacggc
    21421 aacgccttgc cacaattgcg gaggaggcgt ggcgcgaggt gttcggtgcg tctgatcccg
    21481 aggacgaagt cgagaagacc tgggaggtcg tcgccggcac gggcaagtac gcagcgatct
    21541 gggactacga tccggctggg gatagtgcgt atcaggtcgc accgatcatc tctcgtgcgg
    21601 tccgaggcac ggtctacgtc gcgcagctcc acaagcatct gtatggcgtg tcggcgtaca
    21661 agggcggacg cgcgctcgat gatgtggtgg aggccccgct gcggctcgcg aatcggctcg
    21721 gcgtcccgtt cccaccatcg ccggtcgtat cgccggaagc agagtggtcg gtgtgcatcg
    21781 tggacggtgc cccccccgac ctggtgaata gcgccctcga cctgacgccc gaggatcgcg
    21841 ttgtcgtgag agtggatccg catccgctcg gaacgatcat ttacgcgagc gatcgatcgc
    21901 ccgtgctcgg cgtcccgagc atggtcaaag gacgcggcct cgagcacgcc acgacctacg
    21961 ccgtcgagcg ctcgcacggt gacgcgctga ccatctgggt ttaccgcaaa ggcgagatca
    22021 tcttgtattt ctgcacggac gagcgcctcg ggctcggtcc accaaatgcg gaccggacct
    22081 gggacatcaa aggcgcacgg gacgtagacg cgattctcgc ggcgctgagc atcccgcctg
    22141 ccctgctcgg ctggtaatga caaggtttgt cctcgtggac ttttttgtcc accctgcgcg
    22201 ggcgcaagct tgcgtcgagg tgccagacgg aatcgaaccg acgatggtgg ttttgcagac
    22261 cactgccttg ccgcttggcg atggcacctg atgtggaacg aacgttccat gcgcgggaga
    22321 tatgggagtc gaacccggcc actgacgtgg catctcgttt tcgagacgag cgtggcgacc
    22381 aaccgccacc tgatcccccg aatgcaaatg gggatgcgtc gcgggcaact cgccagaacg
    22441 cagacggatc tcgttccagg agtcgcttta gcgactacaa caggatgacg agacgcgcgt
    22501 gtgcatggcg cttcttatag gggcggcgac cgcgatcgtc aacgcgagtt ttcgaacgcc
    22561 gcgaaactgc tagtagtcgt cgcgcagcac gacgttcgag agctcgcgct tgagcatcca
    22621 gttgaagatc tcgggcgcga accaggtgta gccacagaac ccgagccaca ccttgcgcaa
    22681 catgatctcg gcgtcctcga atccgtacgc gtcgagcaag atgatggcga tgcgcacgac
    22741 gacgaagctg ccgatcaaga gaccgatctg cgtccgctcg gaccgcagcc ccacgaacat
    22801 gttgagtcgc caccataggc cgagcaccac gttgcggcgc gccttgattg caacccagag
    22861 ctgcagcgcg tcgcgttcgc cagcatcttg attcagcgcg aaccgcgcgt ggcgctccgc
    22921 ttcgtcgacc tggttgcgtg cgagcgcgat gtagccggcg atgatgtgcg cgtcgacgtt
    22981 gctcggatcc gcggccagcg cttgctcgat gcgatgcagc gcctcgctgt gattgccggc
    23041 gttgagctcg agttgtgcga gctggctgag cgcgtcgacg tgatcggcgt cgatcgcgag
    23101 cgcttcgctg agcagcgtat gtgcagcttg atgatcgcct tgcagcgcct tgatgcctgc
    23161 gccgagcacg cgcgtgtcga catcgtcgtc gtcgtcctgc atcgcgacga gacagtgacg
    23221 ccacgcatcg tcgagcttgc gctcggctcg acgtaccgcc gccgctgcgt agtggcagaa
    23281 cgggttgttc gggccaaagc cgagagcgag atcgacctcg atcacggcgc ccgcaagccg
    23341 gcgtgcaccc agtagcgcga gcgcgagcga tgcgtgtgcg ctgtcgtgct cgggatcgac
    23401 cgcgagcgca cgcttgagca gatcgatcgc ggtgtcccaa ttgtgctggc ggcgcgctcg
    23461 atcggcttgc tcgatcagtg cgcggacctg acgcgattca ctcatgacag cacgacctgc
    23521 cagatcggat gcgacgatac gccgagcacg aggtcgcgca ggtagctgcc ggcgaccagc
    23581 acgtaaaacg ccttctgcgc ggtgccgccg tagtacgtgt acacgtggaa cgtccgcgac
    23641 tggatcttcg tcacggtgta cgcgagtccg agcttgaacg agacgacgct cagcaccgcg
    23701 aactgcaggg cgagcttgga ctcgatgatg ccgagatcga cgaggccgaa caccgccatc
    23761 gccagcgcca acgtgcctgc aaagccgagc gcacacagct tcgcttcgcg gcgcagcgtc
    23821 ggactgccca tcgcgtacgc gttgacgatg taccacggcc acgcgagcca cgcgccgcac
    23881 cacatcatgc cgagcagcgg cgccgtcggc tgaaatacca ggttctccca gcccgcgctg
    23941 ggattgggtt cgtctgcgat ctcgtaggtc atttcttggt gtgcttggca aggaaggcga
    24001 gcacgtcgtc gtactgacca ccttcgttgg cgtaacgcgc gtagttgcgc gcggtggtga
    24061 gccactcact cgttgttggt ttgatctcgg cgagtgcggc aagcagatgg ctatggttga
    24121 tcggcacctc gccactcttg ctgagcgaat cggcgatcgc gcgatcggcc gcagtgtcga
    24181 cgaggttttc gaggtcggcg cccgagaagc ccgacgtccg cgtcgccaac aggtcggtgt
    24241 cgatgtcgtc ggcgaccggc cgaccgttca gcaggatttt caagatcgac gagcgcgccg
    24301 cgcgatcagg cggcggtacg aacagcacgc gatcgaagcg gcctggcctc cggaacgcgg
    24361 agtcgatcgc ccacggcacg tttgacgccg cgaggatgag cacgccggcg ttgttcttcg
    24421 cgaagccgtc catctcggac aagaactggc tgacgagctt cgacgccgtc gcttcgcgcg
    24481 tgtactggcg tttgccggcg agcgcttcga tctcgtcgaa gaacagcacc gccggcgtcg
    24541 tcgcgcgcgc cttctcgaac agcgcgtgca gtttgcgctc gctctcgccg atccacatgt
    24601 cgagcacgtc ctcgatcgcc acgttgagga acgtcgcgtt gcactgaccg gccgttgccc
    24661 gcgcgagcaa cgtcttgccg cagcccggcg gcccgtagag caggatgccg ccacccgcct
    24721 tcttcttgaa cttcgcgaac agcgccggct tctgaaacgg cagaatgatg cgcttctcga
    24781 tctgggtctt gatctcgtcg agaccaccga cgtcggcgaa cgtaaccttc gtcggctcgg
    24841 gatgcagcag ccgatcgact tcgctcgcgt cggtgtcgtc gttggacagc acgcgcagtc
    24901 tcacgccgtc ttcctcgtgc agcttctcga ccgtgccaag cgatagccgc agatcccgat
    24961 cctcgagagt cgggttcgtc cgcaccgccg cttcgtacgc agcaagcgca gccggctgat
    25021 cgttgagcgc gtgtagcgcg cgcgcgcgca ggatcaacac ctcgggcgct tcgcccgtag
    25081 caagctcgag cgcgcgcgtg tgtgctccgc cgtcgagcag cagcttcacg atcgtgacgc
    25141 gcaacgtgac gttctctggc gacgcggtga gcgcgtcgag caatgctttg atcgtggtct
    25201 cgtccatggt tagcgcggaa aggcaacgag ggtggtcgct ccggccgggt ggccgacgag
    25261 caattgatcg cgtgaccacg caagcgcagg tgcatgcttg ctcggcgggc ctgcccagag
    25321 cggcgtgacc acgcccttac gatcgacgcg gccgacctcg gtctcgtgtt caccgacgaa
    25381 gccgaccgcg aacgcgttct cgccagcgcc gacgatcgag acctcgagca gcttggtcgg
    25441 tagtggcttc gtccacttgg tcgctccgct ctcggtcacg aactgcaggc gcgaggcacc
    25501 gttgtggtcg cgcacgccaa gcaagcacgc gttgcggacg caaccgaagc cgagcagcgt
    25561 ctctctgcga tcgagcgaga ctgtcgctgg taggcccgag catttcttgc gcgtgcaggt
    25621 ctggatgaac aagcgatcac tatctcgcca gaacaacgca accaggtcgt gcgccgacgc
    25681 gacgccgcac cagaccttgc gatcggcgtt cgcgagcccg acggcttcgt cggccatctg
    25741 ctcgtcggtt cgcttgttgc gcgtgtcgcc gtggacgaac cagatcgtgt cattggcttc
    25801 gagccatgcg acagcgaagc gttcgtcatt cgtcgcggcg ccctcgcaca gcatgcgcac
    25861 ggagttgccc cacaccgacc ctttgccggt gctcgcgcgg acgagcctga ccttcttact
    25921 ctcgacccac gcgaccgctg ggccattcga cgggcccacg agccgcagct cgccgtcgtg
    25981 cgcgagcacg tgctgcttgc gctgcttgcc cttgcgatcg agctcgacga cacgatgacc
    26041 gtcgtagagc gcaaagccgt cggcgagcac ggccgcgtga cgctcgccat cgtgcgccgc
    26101 cggcgcgtcg ccccgccacc agacccagag accgatcact gccgccgcga gaagccatcg
    26161 ccgcatgggg agtgaagata gatccgccgc gctgcgtttg cagctgtctc gtgcggtgcg
    26221 atcgaggggg gcctatctga ttgacggcag ccgctacgga ggcggcgact gcgtattgct
    26281 gtgcttgcgc cgctttggtt cgtcgcccgg cttcgcatca tcgctcgcct ttgcctcgtc
    26341 gctcggcttc gcgtcgtcgg tgggcttcgc ttcctggctt gctgtgtcgc tcgacgcctt
    26401 cacctcggac gccgtcgcgg gtgcagcgct ctctgcgggc gcgagcgcgg cggcgatcgc
    26461 actctgtttg tcgggctcgt caccgatcgc gagctcgcac tcgccaaata cgacctccat
    26521 gcccttgtcc gtcttgagcg tgacctccag tgagcgagcg cggatgcgag tcttgtcgcg
    26581 aaccgtaccc gcgatctgaa cggtctctgc ttcgacctcg cctgcgagca caccttccga
    26641 acgcagctgc ttcacgttga ccttgcccgc gacgacgccg ctgctcgcaa cgtgaatcat
    26701 cggtccggtc acatcgcctt cgaccttgcc gaccacgacg atcgggcagt tcgaagacag
    26761 cgagcctttg aactcggtgc cctcctcgat gagggtgtgc ttgccggtgg gaaggccgtt
    26821 cttgtcgctc atcgcttcac cttgttgttc cgagttgatg tggaggacac cgggccgacg
    26881 atcttgacgc gtaactggca gccactctcg aacgcgaact gcgtgcgttc ggcgctgtgt
    26941 gcgaccgatg acatgagggc acccttcgac gtgaccgaga gttggcgcag accaacgatg
    27001 cctgggctct ctcgctcgaa gaacttgccg ttgatcgtca cggttccggc tccgatgatc
    27061 tggcaatccg cgagtacacc ggagggcccg acgatcacct ccgtaccgga tccgatttcg
    27121 atcgtgcagt tctgaaacaa actgtcgatc tcgacacgcg caccgtcgtc gaagtgcagt
    27181 gcggcatcgc ggagcacgtc accgcgaccg aagtagcggt tgcgcgcttc ggtgcgcgtc
    27241 ggaactgcga gtgcgccgcc gctcaggagc gacggaatca cgccgttctc gacccgctgc
    27301 gaggcatcgc ccgagggact gacgtcgagc caagccacct ccatgggacc aagcggcacg
    27361 ccggtgccga tgagacctgc gatcttcgcg ccggtcaggt tcgcagcggt cagttgcgcc
    27421 cgcccgaggt tcgcgaaccg cacatccgcc gacatcaggc gtgcgccggt cagatccgcg
    27481 cccgtcagat ccgctttgac gagcgtcgcc tcgacgagat cgacgttgcg caggatcgca
    27541 tggcgaaggt tcgcgtcctc gaacgacgcc ttctgcagct tcgcgtcctc gaggttggcg
    27601 cgcaccagcg tcgcgaactc gaagtgagca ccctgtgcgc tagcgccgcc gagatacgct
    27661 tcgtcgagct cggcatgcgt gaacgccgca ttgtcgagct tggcgccgcc gagcttggca
    27721 gtcgcgagct gcgcgtagct gagctgcgcg cccgacaggt cggcattgtc gagcacgaca
    27781 ccctcgaggt tcgcacgcga cagattcgcg cccgcgaggt tcgcgcgcga caggttcgcg
    27841 ccttcgagat ccgaggtctc gagatttgcg ccggccagct tcgcacccac gaggtacgcc
    27901 tcgcgcagcg ttgcacgccg caaggtcgca cccgtcagat cggcttcctc gagatttgca
    27961 ccgtcgagat ccgcgcggtc gaacccagct ttcgcgagct gggtacggga gagattgatg
    28021 ccgcgcaaat ctgcgcgctc catgcgttct ccccgctgga tccttttcag cacctcttcg
    28081 cgtgatggtc ctgccatcgg ttctcctatc ggacctgtga ctgtgcgctc acgtaaagcg
    28141 cgagcttctc gacccgttcg tgaaatggca gtcgttccaa ggtggacaac tgatcgcgcg
    28201 caccaagccg tgcgagcgcg accttgatgc gctggaacgt ctgttcgcgc tgcttgcgta
    28261 gctcgcgctt gcgagcgacc gcgtcttcac tccatgcatt cgcggcgcgc gagaaatcct
    28321 cgttctgatc gccgagcgac ttcacgagct gggccagctc gccgagctcc gccgcgggat
    28381 cgcgcaccga aatcgccgcg tgcgtgcggc tgcggtagcc cttgaactgc gaaacgatcg
    28441 cctcggcgat cgcagggcgg attccggaga ccaccgcgac ctcttccgca ctcgcgttcg
    28501 tcagcgcgtc gacacgattt agtccgaccg cgaacagctt ggcgatcgtc ggccgctcga
    28561 cgccctcggt ctgcaagagc agcgcctcga cgatgatcgg ttcgcggcgg tcgcgctcgg
    28621 catcgagctc gaatgccttc ggaatgagct cgatgagccg cccgtaacgt tgcagcagca
    28681 cggccttgtc gtcgtcggag atccgagcgc gatttgcgac cgccttgtcg atcgtcgcgg
    28741 cgaattcatc gagcgcctta cacaggtccg cgagatcgat ctgtaccgcc atcgcgcgga
    28801 gcgagctcaa cgccggtttg gtcgccgcga gccacgtcgg atctgcgtcg ccgaagcgaa
    28861 gctcgagcat cacgtcgcgg acttgcgaga cgtgcgcgac cgcgacttca tcgaatgtct
    28921 tgcggaccgc ttccaggtcc tccgccgtcg agacgccctg cactccggcg gatttgccgg
    28981 cgaccatatt ctcgaacgct tcgctgacgg agtccgagtg cggacgccgc gcgctcgcgg
    29041 acgattctgc gacggtggaa gccgacacag gctgcgccgt ggctgtcggt agcgacgcct
    29101 gcgcactagc tcgggtcgca tgtgcatgtc gaggcgtgga tggcgtcgtc gttggcgtag
    29161 tcgttgccgg tgtactcgtc gccggtgtcc ccaccgccgg cgttcccagc gccgaacgga
    29221 tcggcgtgcc ctgcggtggc gtcttcggct tgacgacgct aggcggcaac gccgagctcg
    29281 gtcgggccgc agctaacgac gacgaggatg ttgcgacggg tggcgcgctc ggcgctcggt
    29341 tcttcgtcat cgccggcgtc gcctcgggct tcgacgtcgt cgcggagggt gtcatcggcg
    29401 tacgcgtgct gttgctgagt ggcatcgcgc gaggcaccgg ttgcgacggc tcgcgacgaa
    29461 ccggcacggg cgcggcagac ggaacagcct tgttcgcggc gggcgcgctc gcggttgcag
    29521 tcggcgacga cggtgacgcg gccgcggtga cagcagacga aggcgcggcc ggcgcgcttg
    29581 gctcgagctg aggtgcgtg
//
LOCUS       DASUFX010000791         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1657715, whole genome shotgun sequence.
ACCESSION   DASUFX010000791 DASUFX010000000
VERSION     DASUFX010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657715"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VFV99_31955"
     CDS             <1..1878
                     /locus_tag="VFV99_31955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064051.1"
                     /translation="KKTQLGTAPGRTVRPTGSQTPVAAAGTPVPATIDAAKAKTSYLP
                     DVPDPLLGATVAGRYKIIRKLGEGGMGSVYLAMHTILEKQVALKVLHGEFARKPDLVD
                     RFMQEAKAASRIRHENVIDISDFGVTPDGHVFFAMELLQGHDLHDSIARAKLAGQLLP
                     WSRTKKIFLQICGALAAAHARGIVHRDLKPENVYLIDFLGDPDFVKLLDFGIAKLTDV
                     SNEEGGRKLTKTGMLFGTPEYMSPEQARGENVDHRVDVYAMGCILFQLVTNRVPFEAD
                     NFMGVLSQHLTEQPPSIPPQVFDQIGAPRALADVIDKALEKNRDARWQTIEEFAAAVR
                     QVSGDPPAADKSQPMRAANVASPSETTRARPPTGPVVGRQRSETGRVKTQWTGNLHVP
                     EAAEPTAKPKSKAPLIIGLGVLVVGGAVAAVVALKGGGGGAGEGSAVASGSAPAVAPV
                     VQPPVQPPPPPPPPPPKDVLPEQSEIVLTSDPKGAVVTDLSNNRVLGTTPQRFKIAGS
                     TTPRQFKFTLKGYGDSTVELVPNRALIEYAETLHKGATKPNAVTKVPEQKPADKPGDA
                     VTKPDTPEIKPVDIKPADTKPADTKPADTQKPPDKPKDDCPEDEQPCLKTHIPGMGSD
                     K"
     gene            1917..2840
                     /locus_tag="VFV99_31960"
     CDS             1917..2840
                     /locus_tag="VFV99_31960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5064052.1"
                     /translation="MTTGRMRVASYTARDGSTKDVEIAYDVFGTRGKPLVLIMGIGAQ
                     RIFWDNAMCEQFVDAGFQVVRFDHRDIGHSTKLDAKVPPPMKTLIRGMAKVSVDAPYD
                     LSDMAHDVIQLIKGLGWQDAHMVGVSMGGMIGQHLAMEHGDRVRSLTTIMTTPGGRRY
                     MPEPYALRALFAPAPKNAEEAGLHVDKLFSLIGSKAWPHDGERLRAIGMEAHARGMNP
                     RGFLRHFAAVMASGDRREKLRDVKVPTLVIHGSKDPMFPLSAGRKIAQLTHGTWLPIA
                     GMGHDMPEPLWPTIVAAIRRHAERADSRATK"
     gene            2848..2930
                     /locus_tag="VFV99_31965"
     tRNA            2848..2930
                     /locus_tag="VFV99_31965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2881..2883,aa:Leu,seq:taa)
     gene            complement(2931..3767)
                     /locus_tag="VFV99_31970"
     CDS             complement(2931..3767)
                     /locus_tag="VFV99_31970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX5064053.1"
                     /translation="MPPKTWTDDQLRVVVAESRTWASVLRSLGLSPRGRNHYTITARV
                     RHLGIDTSHFSMSSRPAEDDQLRRAVAESSSIEGVLDSLGLDQDRDTRERLKRRIRAL
                     GIDTGHFARTQVTPSRKRTRRWDDEKLRAAVAAAQSMAGTLRNLGLIAAGGNYDAVQR
                     RIKELAIDTTHFTGQGWNVGLKYQPAPAQPIEQLLVRDRWTTSHHLKTRLIRKGLKQA
                     KCELCGWCERAPDGRIPVELDHINGDRVDNRLENLRILCPNCHSLQPTHRGLNQKRRR
                     RS"
     gene            complement(4063..4308)
                     /locus_tag="VFV99_31975"
     CDS             complement(4063..4308)
                     /locus_tag="VFV99_31975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064054.1"
                     /translation="MKFNTLTHEITVEPLHVEEGEDLAAAHQRIIDNCPECQAARARG
                     EPPVTLTGEQLRAQMRPRRDIIRDRRPRWRDLKNGRR"
     gene            4444..4973
                     /locus_tag="VFV99_31980"
                     /pseudo
     CDS             4444..4973
                     /locus_tag="VFV99_31980"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            complement(4970..>5428)
                     /locus_tag="VFV99_31985"
     CDS             complement(4970..>5428)
                     /locus_tag="VFV99_31985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064055.1"
                     /translation="EPADLRGLVGQRDSRDPYVIVLDWLDEVGIDIHPPETAGEPLVT
                     WAQTAHMLAAPTDIAKPGDLVVFDRALGDDLFDLVAIVIGRDARGVTEFMYAGGGVIR
                     RGFLDPTHASSRRDVDGLVLNTFLRHGKRWPPKGTRYLAGELLSHVIHTH"
BASE COUNT          960 a         1727 c         1836 g          905 t
ORIGIN      
        1 aagaagacgc agctcggaac agctccgggt cgcaccgttc gccccaccgg cagccagacg
       61 cctgtcgccg cggccggcac gcccgtgccc gccacgatcg atgcggcgaa ggcgaagacg
      121 tcgtacctgc ccgacgttcc cgacccgttg ctcggcgcga cggtcgcggg tcgctacaag
      181 atcatccgca agctcggcga aggtggcatg ggctcggtgt atctggcgat gcacacgatc
      241 ctggagaagc aggtcgcgct caaagtcctg cacggcgagt tcgcgcgcaa accggacctg
      301 gtcgatcgct tcatgcagga agcgaaggct gcgtcgcgca tccgtcacga gaacgtgatc
      361 gacatcagcg acttcggcgt cacaccggac ggtcacgtgt tcttcgcgat ggagctcttg
      421 caaggccacg accttcacga ctcgatcgcg cgcgcgaagc tcgcaggcca gctgctgccg
      481 tggtcgcgta ccaagaagat cttcctgcag atctgcggcg cgcttgcggc ggcacatgcg
      541 cgcggcatcg tgcatcgcga cctcaagccc gagaacgtct acctgatcga cttcctcggc
      601 gatccggact tcgtgaagct gctcgacttc ggcatcgcga agctcaccga cgtatcgaac
      661 gaggagggcg gccgcaagct gacgaagacc ggcatgctgt tcggcacgcc cgagtacatg
      721 tcgccagagc aagcgcgtgg cgagaacgtc gaccatcgcg tcgacgtcta cgcgatgggc
      781 tgcatcctgt tccagctcgt caccaatcgc gtgccgttcg aagccgacaa cttcatgggc
      841 gtgctgtcgc agcacctgac cgagcagccg ccgtcgattc cgccgcaggt gttcgatcag
      901 atcggcgcgc cgcgtgcgct cgccgacgtg atcgacaagg cactcgagaa aaatcgcgac
      961 gcgcgctggc agacgatcga agaattcgcg gcggccgttc gccaggtgtc gggtgatccg
     1021 cccgcggccg acaagtcgca gccgatgcgc gcggcgaacg tcgcgtcgcc gagcgagacc
     1081 acgcgtgcgc gtccgccgac gggacctgtc gtgggacgtc agcgctccga gacgggccgc
     1141 gtgaagacgc agtggacggg caacctgcac gtgccggaag cggccgagcc gacagcgaaa
     1201 ccgaagtcga aggcgccgct gatcatcggg cttggtgtgc tcgtcgtcgg tggcgcggtc
     1261 gccgcggttg tcgcgctgaa gggcggcgga ggcggagcag gcgagggcag tgccgtcgcg
     1321 agtggctcgg cgcctgcggt cgcgcccgtc gtgcagcctc ccgtgcagcc gccaccgcca
     1381 ccaccaccac cgccaccgaa ggacgttctc cccgagcaaa gcgagatcgt gctgacgtcg
     1441 gatccgaagg gcgcggtcgt aacggatctg tcgaacaacc gcgtgctcgg cacgacgccg
     1501 cagcgattca agatcgccgg ctcgacgacg ccgcgtcagt tcaagttcac gctcaagggc
     1561 tacggcgaca gcaccgtcga gctcgtgccc aatcgcgcgc tgatcgagta cgcggagacg
     1621 cttcacaaag gcgcgacgaa gccgaacgcg gtgacgaagg ttccggagca gaagccggcc
     1681 gacaagccgg gcgatgcggt cacgaagcca gatacgccgg agatcaagcc ggtcgacatc
     1741 aagccggccg atacgaagcc cgccgacacg aagcccgcag atacgcagaa gccgccggac
     1801 aagccgaagg acgattgtcc tgaggacgag cagccttgcc tgaagacgca cattccgggc
     1861 atgggcagtg ataagtaagc agcgtgggcg cagcttctcg ctttgaatcc gtcgcgatga
     1921 ctaccggccg catgcgcgtg gccagctaca cggcgcgtga tggatcgacg aaggacgtcg
     1981 agatcgcgta cgacgtgttc ggcacgcggg gcaagccgct cgtcctgatc atgggcatcg
     2041 gcgcgcagcg catcttctgg gacaacgcga tgtgcgagca gttcgtcgac gccggctttc
     2101 aggtcgtgcg cttcgatcat cgcgacatcg gccactcgac gaagctcgac gcgaaggtgc
     2161 cgccgccgat gaagacgttg atccgcggca tggcgaaggt gtcggtggac gcgccgtatg
     2221 acctgagcga catggcgcac gacgtgatcc agctgatcaa aggcctcggc tggcaagacg
     2281 cgcacatggt cggcgtctcg atgggcggca tgatcggcca gcacctcgcg atggagcacg
     2341 gcgatcgcgt gcgcagcctg acgacgatca tgacgacgcc gggcggtcgc cgctacatgc
     2401 cggagccgta cgcgctgcgc gcgctgttcg ccccggcgcc gaagaatgcc gaggaagcgg
     2461 gcctgcacgt cgacaagttg ttctcgctga tcggcagcaa ggcgtggccg cacgacggag
     2521 agcgcttgcg tgcgatcggt atggaggcgc atgcgcgggg catgaatcca cgcgggttct
     2581 tgcggcactt cgcggcggtg atggcgtcgg gcgatcgacg cgagaagcta cgcgacgtga
     2641 aggtgccgac gctcgtgatc cacggcagca aggacccgat gttcccgctg tcggcgggcc
     2701 gcaagatcgc gcagctgacg cacggaacgt ggttgccgat cgcggggatg gggcacgata
     2761 tgcccgagcc gttatggccg acgatcgtgg ctgcgatcag gcgccatgcc gagcgtgctg
     2821 attcgcgtgc tacaaagtga acgcatcgcg cgagtgccgt aacggtagcc ggtgggtcct
     2881 taaaaggctc tgcccgaaag ggcgtgcggg ttcgaatccc gcctcgcgca ctacgatctc
     2941 cgtcgacgtt tctgattcag accacgatgc gtcggctgca agctgtggca attcggacag
     3001 aggattcgca ggttctctag tcgattgtcg acgcgatcgc cgttgatgtg atcgagctcg
     3061 acgggaatac gtccgtcggg tgcgcgttcg caccaaccgc agagctcgca cttcgcttgt
     3121 ttgaggccct ttcgaatcaa gcgtgtcttg aggtggtggc tggttgtcca gcggtcgcgt
     3181 accaatagtt gttcgatcgg ttgtgcgggt gcgggctgat acttcagacc gacattccag
     3241 ccttgccccg tgaagtgcgt cgtatcgatc gcgagctctt tgatccgccg ttgaacggcg
     3301 tcgtagttgc cacctgccgc gatgagtccc agattccgca gcgtgccggc catgctttgc
     3361 gcagctgcga ccgcagcgcg cagcttctcg tcgtcccagc gccgcgtcct cttgcgactc
     3421 ggagttacct gcgttctcgc aaagtgacct gtgtcgatgc ccagcgcacg gatgcgtcgt
     3481 ttcaatcgct cgcgcgtgtc gcgatcctga tcgagaccga gcgagtcgag aactccttca
     3541 atgctcgacg actcagcaac cgcacggcgc agctggtcgt cttcggcagg gcgactgctc
     3601 atgctgaaat gacttgtatc gattcccaag tgcctcactc tcgcggtgat tgtgtagtgg
     3661 ttccgtcccc gaggactgag gcccaagctg cgcaggaccg acgcccacgt tcgtgattcg
     3721 gccacgacga cgcggagttg gtcatccgtc catgtcttcg gcggcatgac tcaccgcccg
     3781 tcgcgcttcg gctgttcagc cgcgcgtgct cgctcagcgc ggcattggat gcagtcgatc
     3841 ggtagcggct ttgcaggctg ctggggatcg aagggcagcg acgagctcgt cacgttgcgg
     3901 gttcggggtt ggccggtaag cgaatcgatg aagacgtgtt tgcgcatggc ggctgcgcct
     3961 cgcaacgact gtgccgcaag caagcgcgtg gaatctcgaa gcgcgacgcg cgggttcgtc
     4021 cgcaacctgg acgccggcag atcgagacgc cactcgcatc gcttaccgcc gtccgttctt
     4081 gagatcgcgc caacgcggac gacgatcgcg aatgatgtcg cgtcgtggac gcatctgcgc
     4141 gcgcagctgc tcgccggtga gcgtcaccgg cggttcgccg cgtgctcgcg cggcctggca
     4201 ctcggggcaa ttgtcgataa ttcgctggtg cgcagccgct agatcttctc cttcttcgac
     4261 gtggagcggt tcgacggtga tctcgtgagt gagcgtgttg aatttcatgt ggtggcgcat
     4321 atcggccgcg catcgcaacg gatgtgccgc cactaaccgc gtggaatcgc ggaacgcggt
     4381 cgtgaatggc gtccgcagcc tggacgccgg ccattcgagt cgccgcgtgg cagaatgccg
     4441 gcgatggcgt ggctgctact cgcgagacac ggcgagaccg actggaatcg cgagggccgg
     4501 tggcaaggtc acgccgacgt cgcgctcaac gatcgcggcc gcgaacaggc gcgcgagctc
     4561 gcgatgcggc tcgccgatca accgatcgac gtcatctact cgagcgacct caaacgtgcg
     4621 cacgagaccg cgctgatcat cgcggagaag aagcgcatgc cggtcgcgac cgacgcgggt
     4681 ctgcgcgaga tcgacgtcgg ccgctggtcc ggcctcacgc ccgacgagat cgcgcgctgc
     4741 tttccggcat gacaacgcac gacggcgaga ccaacgagca acacctggcg cgcgtgctcg
     4801 acgcatttcg ccggatcgca accgcggagc tcgaccgtcg cgtgctcgtc gtctcgcacg
     4861 gcaagacgct gcgcgtgatc cgccgccatg cagccggcaa acccgtctcg ctgctcggca
     4921 actgcgaaac agcgcgcatg cgcttcgtcg acgacaaatt cgtagacgat tagtgcgtgt
     4981 gaatcacgtg cgagagcagc tcgccggcga ggtagcgcgt gcctttgggc ggccagcgct
     5041 tgccgtgacg caggaacgtg ttgagcacga ggccgtcgac gtcgcggcgc gatgacgcgt
     5101 gtgtcggatc gaggaagccg cgccggatca cgccgccgcc ggcgtacatg aactcggtga
     5161 cgccgcgcgc gtcgcgcccg atcacgatcg cgacaagatc gaacaggtcg tcaccgaggg
     5221 ctcgatcgaa cacgaccagg tcgccgggct tcgcgatgtc ggtcggcgcc gcgagcatgt
     5281 gcgcggtctg tgcccaggtg acgagtggct cgccggcggt ctctggcgga tggatgtcga
     5341 tgccgacctc gtcgagccag tcgagcacga tgacgtacgg atcgcgtgag tcgcgctggc
     5401 cgaccaggcc gcgcagatcg gcgggctc
//
LOCUS       DASUFX010000792         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_126369,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000792 DASUFX010000000
VERSION     DASUFX010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126369"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFV99_31990"
     CDS             complement(<1..226)
                     /locus_tag="VFV99_31990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064056.1"
                     /translation="MDCPDDNVLVAMVEQALDPANFAEIEVHIDSCEHCRKIVAAALA
                     SRSLALGTPKPEDVAEALVPAIDVSISDRYV"
     gene            complement(226..1503)
                     /locus_tag="VFV99_31995"
     CDS             complement(226..1503)
                     /locus_tag="VFV99_31995"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX5064057.1"
                     /translation="MRSSWLLGWSLILMSACAVDDYVEDPADDTWLDGKADGATAVDI
                     KATNLDIDLAHKSAVATIELEKAGNVELEAGGLHITKITDSRGKRRYTLTNGKLRISN
                     VRGDLVVEYTFDQHTNADGLLPGGSTVLWPYYCGNLFPCHSRPADGTTFALSLDGVPD
                     SKTAIYPEEIAAESPSYMLAWAVGSYTKKQLGETTAGTKVSTAWLPGGETAALAGTKH
                     LVQAFDWYEQNLGPYSFGHDVASVSVVWGEGMYGGMEHHPYWHVAKDAMNDEVTHVHE
                     AAHGWFGDGVRLRCWEDFVLSEGTVSYLAARALGKVAGAAKEASVWAEYKSELDDAIA
                     EGGAPAWPTGCNKIDILRDKLFTNLPYMQGAYFYKDVAAQIGADKLDQIIGDFYKAHR
                     NKPAGMQDMIDAIKAASGFDPTPIAEARLRKRF"
     gene            complement(1585..2250)
                     /locus_tag="VFV99_32000"
     CDS             complement(1585..2250)
                     /locus_tag="VFV99_32000"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5064058.1"
                     /translation="MSDPALGEETPWRVLGTRGCGSAIVEAALVLAKIPYTREEVDYT
                     TAAGHDKLALVNPLAQVPTVVLPDGTVMTESAALVLYIDGLVPELELVPSVRDPLRRE
                     LLRWLMFLVAAVYPTFTYGDEPGKWVGDAGPSLRESTDEHRKSLWRLVEGAVRGPWFL
                     GETPSALDLYVSVMTRWRPRREWFGEHCPRLYKIATAVDADARLAGVWATNFGESPEQ
                     QTA"
     gene            complement(2247..3155)
                     /locus_tag="VFV99_32005"
     CDS             complement(2247..3155)
                     /locus_tag="VFV99_32005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5064059.1"
                     /translation="MLTIDLTGKHALVAGVADDGGFGFAIAKALAEAGAKVSVATWPP
                     ALGIFKTMLERGKFDESMKLASGGKLAFQKIYPLDAEFDRLEDVPAELKDNRRYRDAG
                     DFTIGGLAAAMKADLGVGGLDIVVHSLANGPEVKKPLLEVSRKGYLGALSASAYSMVS
                     MVSHFSPLMRRPGSFISLSYMASERTVPGYGGGMSSAKAALESDTRTLAFEAGRKFGH
                     RVNVISAGPYASRAASAIGFIDQMVDYAKRNSPLPEPITAEEVGATAAFLCSPLASGI
                     TGATIYVDKGMHTMGLAVDREGAVKG"
     gene            3366..4700
                     /locus_tag="VFV99_32010"
     CDS             3366..4700
                     /locus_tag="VFV99_32010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064060.1"
                     /translation="MRIAVLLAALALPLTASADPSFNDRVMQPPKRLDANAPRSFTNV
                     SHLVYLNRCPGGCVVSPGFDDAKTNHSSIPNSQATLTAFPLGDPAWNRLVQCVRDGFA
                     PFDIQVTDVDPGSANHFEVMIAGNATAIGVPGAGGVAPFEPCDGQLQDNVISFVFAAD
                     INNEDFLCWAALQEPSHLFGLDHLLDAKDPMTYLTPPIKKEGFQNTANNCGEDTPREC
                     WCGGTKQNSYQYLMDTFGPSHLEPATMAITEPADGAWVKPGFNVRFAVMSQLSVTSAS
                     LQVDGTTTQSIAQGDPQVFATSTSLTGGDHTISVTAKDSANRDVSGQITVHVVDRCDG
                     GSACAKDFHCLGGYCLPGGKVPGGLGAECTGNESCITGQCASDGTSMVCTGNCDEGMT
                     CPGGFSCVGTSPGMGVCWPAPDDGGCSTSNDHSPLFALLGLGALVMIVRRRK"
     gene            4713..5714
                     /locus_tag="VFV99_32015"
     CDS             4713..5714
                     /locus_tag="VFV99_32015"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5064061.1"
                     /translation="MNRFRRAAIRAAMKSEWFVRFAGRGRSEGPDEALDRQIAAALEY
                     QRVARLPPLESLDPVAARAFAESNLEATELAPESMAEIIDTTLTHDRIPARIYVPQDA
                     GKHWIVWCHGGGGVIGSIEGSDRVTRYLAEHTKCTVASVGYRLAPEDKHPAAIDDAVA
                     AWEALTERMPAGARAAIGGDSFGGFLSVHVDRETRVRGLRQPDLQILVYPLVDMTLTQ
                     PSIDRYAEGYLLTKAMMNYFRDHYLNNADERQTGSPWFWKDVSGAAPAIISTAGYDPL
                     VDEGNAWAERLAAAGVTVRHHCHPGLVHGFLSLAGIVRAAKSATDQICVEIVELLSE"
     gene            5727..>6193
                     /locus_tag="VFV99_32020"
     CDS             5727..>6193
                     /locus_tag="VFV99_32020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064062.1"
                     /translation="MHVLIAVADVGAGVQLEEALTQAGFAARWDGNQADGPRGASQQE
                     VVLIDADRLGENLCSVTDAWRDHLSVPGVVALGTSATAREQAPIARVTLLSPSAQTST
                     IVKAIREAAKLRLSVGMRWPVLRAALKLPPAANTPEAWPAVVLHSRNADLEVAR"
BASE COUNT         1026 a         2063 c         2030 g         1074 t
ORIGIN      
        1 cgacgtagcg atcgctgatc gagacatcga tcgcggggac gagcgcctcc gcaacgtcct
       61 ccggcttcgg cgtgccgagc gcgagcgagc gcgacgcgag tgccgctgcc acgatcttgc
      121 ggcagtgctc gcagctgtcg atgtggacct cgatctcggc gaagttcgcc ggatcgagcg
      181 cctgctcgac catcgcgacg aggacgttgt cgtcaggaca gtccactaga acctcttgcg
      241 cagcctcgcc tcggcgatcg gcgtcggatc gaaaccgctc gccgccttga tcgcgtcgat
      301 catgtcctgc atgcccgccg gcttgttgcg atgcgccttg tagaagtcgc cgatgatctg
      361 gtcgagcttg tcggcaccga tctgtgccgc cacatctttg tagaagtacg cgccctgcat
      421 gtacggcagg ttcgtgaaca gcttgtcgcg caggatgtcg atcttgttgc agcccgtcgg
      481 ccatgccggt gcgccgccct cggcgatcgc gtcatcgagc tcgctcttgt actcggccca
      541 caccgacgct tctttcgccg cgcccgcaac cttgccgagc gcgcgcgcgg cgagatagct
      601 gaccgtgcct tccgacagca cgaaatcttc ccagcagcgc aaccgcacgc catcgccaaa
      661 ccaaccgtgt gccgcctcgt gcacgtgcgt gacctcgtcg ttcatcgcgt ccttcgcgac
      721 gtgccaatac gggtgatgtt ccatgccgcc gtacatgcct tcgccccaca cgacggacac
      781 cgacgcgacg tcgtgcccga acgagtatgg accgagattt tgctcgtacc agtcgaatgc
      841 ttggaccagg tgcttggtgc cagcgagtgc tgccgtttca ccaccgggga gccacgccgt
      901 cgagactttc gtgcccgccg tcgtctcgcc gagctgcttc ttcgtgtagc tgccgacggc
      961 ccacgcgagc atgtacgacg gcgactccgc tgcgatctcc tcgggataga tcgcggtctt
     1021 gctatcaggc acgccgtcga gcgacagcgc gaacgtcgtg ccatcggcag gacgcgagtg
     1081 gcacggaaac aggttgccgc agtagtacgg ccacagcacc gtcgagccgc cgggcaacag
     1141 gccatcggca ttcgtgtgct ggtcgaacgt gtactcgacg acgagatcgc cgcgcacgtt
     1201 cgagatgcgc agcttgccgt tcgtgagcgt gtagcgccgc ttgccgcgcg agtccgtgat
     1261 cttcgtgatg tgcagaccac cggcttcgag ctcgacgttg ccggctttct cgagctcgat
     1321 cgtcgcgacc gcgctcttgt gcgcgaggtc gatgtcgaga ttcgtcgcct tgatgtcgac
     1381 cgcggtggca ccgtcggcct tgccatcgag ccaggtgtcg tcggccggat cctcgacgta
     1441 atcgtcgacc gcacaggccg acatcaagat caacgaccac ccgaggagcc agctcgaacg
     1501 catggccccg gcattcagca agcagagggc caggtccgac caatgcgaag tcgcgacctt
     1561 gcgaggcggt ggctactaga gctgctaggc cgtctgctgt tccggggact caccgaagtt
     1621 cgtcgcccaa actcccgcca ggcgggcgtc agcgtcgacg gcggtcgcga tcttgtagag
     1681 gcgggggcag tgctcgccaa accactcgcg gcgcggccgc cagcgggtca tcaccgagac
     1741 gtagagatcg agcgcgctcg gcgtctcgcc gaggaaccac ggaccgcgca ccgcgccctc
     1801 gacgagccgc cacagtgact tgcggtgctc gtccgtcgat tcgcgcagac tcggtcccgc
     1861 atcgccaacc cacttaccgg gctcgtcgcc gtacgtgaac gtcgggtaga ccgcggcgac
     1921 gaggaacatg agccagcgca acagctcgcg gcgtagtgga tcgcgcacgc tcggcacgag
     1981 ctcgagctcg ggcacgagac catcgatgta gagaacgagc gccgcgctct cggtcatcac
     2041 ggtgccgtcg ggcagcacga ccgtcggcac ctgtgcgagt ggattgacta gggcgagctt
     2101 gtcgtggccc gcagctgtcg tgtagtcgac ctcttcgcgc gtgtacggaa tcttcgcgag
     2161 cacgagagca gcctcgacga tcgcagagcc gcagccgcgc gtgccaagca cacgccaggg
     2221 cgtctcttcg ccgagcgccg ggtcgctcat cccttcacgg ctccttcgcg atcgaccgcg
     2281 agccccatcg tgtgcatgcc cttgtcgacg tagatcgtcg cgccggtgat gccgctcgcg
     2341 agcgggctgc acaagaacgc ggcggtcgcg ccgacttctt cggcggtgat cggctcgggc
     2401 agcggcgagt tgcgtttcgc gtagtcgacc atctgatcga tgaagccgat cgcgctcgcg
     2461 gcgcgcgacg cgtacgggcc tgccgaaatc acgttgacgc gatggccaaa cttgcggccg
     2521 gcttcgaacg cgagcgtgcg cgtatccgat tcgagcgcgg ccttcgcaga cgacatgccg
     2581 ccgccgtagc ccggcacggt gcgctcgctc gccatgtacg agagcgagat gaacgagccc
     2641 ggacgccgca tcagcggact gaagtggctg accatcgaca ccatcgaata cgccgacgcc
     2701 gacagcgcgc cgagatagcc tttgcgcgag acctcgagca gcggcttctt cacttcgggg
     2761 ccgttcgcga ggctgtgcac gacgatgtcg agcccaccta cgccgaggtc cgctttcata
     2821 gcagcggcaa ggccgccgat cgtgaagtct cccgcgtcgc ggtagcggcg gttgtccttg
     2881 agctcggccg ggacgtcttc gaggcggtcg aactcggcgt cgagtggata gatcttctga
     2941 aacgcgagct tgccgccact ggcgagcttc atcgactcgt cgaacttgcc gcgttcgagc
     3001 atcgtcttga agatgccgag cgcgggcggc cacgtcgcga ccgagacctt cgcacccgcc
     3061 tccgccaagg ctttcgcgat cgcgaagccg aatccgccat cgtcggcgac gcccgccacg
     3121 agcgcgtgct tgccggtgag atcgatcgtc agcatggggg cggagtcatc ccgcggattg
     3181 gcccgattcg caagcgagca aacccttcct ggcgccgctg taacgctgcg ggatctcaga
     3241 aattcgagag tcgtgaccgt tggcaccatc tgttgatgca tacgcatcgc gattagtgcg
     3301 ccggggttag caggcgtcgg cgatttgcca gctctggatc gaacacccac gagtacgttc
     3361 cgcccatgcg aattgctgtt ttgcttgccg cgctcgcgct tccgctcacc gcgtccgctg
     3421 atccgtcgtt caacgaccgc gtcatgcagc cgccgaagcg cctcgatgcg aacgcgccgc
     3481 gctcgttcac gaacgtctcg cacctggtct acttgaaccg ctgcccgggt ggctgcgtgg
     3541 tgtcgccggg cttcgacgac gcgaagacga accactcgag cattccgaac tcgcaggcga
     3601 cgctgacggc attcccgctc ggcgaccccg catggaatcg gctcgtccag tgcgtgcgcg
     3661 acggcttcgc gccgttcgac atccaggtca ccgatgtcga tccgggttcc gcgaaccact
     3721 tcgaagtgat gattgcaggc aacgcgaccg cgatcggcgt gccgggtgcc ggcggcgtcg
     3781 cgccgttcga gccgtgcgac ggccagctgc aagacaacgt gatcagcttc gtgttcgcgg
     3841 ccgacatcaa caacgaggac ttcttgtgct gggccgcgct gcaggagccc tcgcacctgt
     3901 tcggcctcga tcacctgctc gacgccaaag atccgatgac gtatctgacg ccgccgatca
     3961 agaaggaggg ctttcagaac acggcgaaca actgcggtga agacacgccg cgcgagtgct
     4021 ggtgcggcgg tacgaagcag aacagctacc agtacctgat ggacacgttt ggcccgtcgc
     4081 atctcgagcc cgcgacgatg gcgatcaccg agccggccga tggcgcgtgg gtgaagcctg
     4141 gcttcaacgt gcgcttcgcg gtgatgtcgc agctcagcgt gacgagcgca tccctccagg
     4201 tcgatggcac gacgacgcaa tcgatcgcgc agggcgatcc gcaggtgttc gcgacgtcga
     4261 cgagcctcac cggcggcgat cacacgatca gcgtgaccgc gaaggactcg gcgaaccgcg
     4321 acgtcagcgg gcagatcacc gtgcacgtcg tcgatcgctg cgatggcggc agcgcttgcg
     4381 cgaaggactt tcactgcctc ggcggctact gcctgcccgg cgggaaggtg cccggcggcc
     4441 tcggcgctga gtgcacgggc aacgagagct gcatcactgg ccagtgcgcc agcgatggca
     4501 ccagcatggt gtgcaccggc aactgcgacg agggtatgac ctgccctggc ggattctcgt
     4561 gcgtcggcac gtcgccgggc atgggcgtgt gctggccggc ccccgacgac ggcggctgct
     4621 cgacgtctaa tgatcattca ccgctgttcg cgctgctcgg tctcggcgcg ctcgtgatga
     4681 tcgtccgccg ccgtaaatag tatcttcggc gggtgaatcg attcaggcgc gcggcgatcc
     4741 gagccgcgat gaagagcgag tggttcgtgc gcttcgcggg gcgcggccgg tccgagggtc
     4801 ccgacgaagc gctcgatcgc cagatcgctg ccgcgctcga atatcagcgg gtggcgcggc
     4861 tcccgccgct cgagtcactc gatcctgttg ccgcgcgtgc gttcgccgag tcgaacctcg
     4921 aagcaaccga gctggcaccg gagtcgatgg cggagatcat cgacacgacg ctcacgcacg
     4981 atcggattcc ggcgcggatc tacgtgccgc aagacgcggg caagcactgg atcgtctggt
     5041 gccacggcgg cggcggcgtg atcggatcga tcgaaggctc cgaccgcgtg actcgctatc
     5101 tggccgagca caccaagtgc acggtcgcct cggttggcta tcggctggcg cccgaggaca
     5161 agcatccggc cgcaatcgat gacgcggttg ccgcgtggga agcactgacc gagcgaatgc
     5221 cggccggggc gcgcgctgcg atcggcggcg acagcttcgg tggcttcctt tcagtgcatg
     5281 tcgatcgcga gacccgcgtg cgcggcctgc ggcagccgga tctgcagatc ctcgtctacc
     5341 cactggtcga tatgacgctg acgcagcctt cgatcgatcg ctatgccgag ggctacttat
     5401 tgacgaaggc gatgatgaat tacttccgcg accactacct gaacaacgcg gacgagcgcc
     5461 agaccggctc gccgtggttc tggaaggacg tcagcggggc cgcgcctgcg atcatctcga
     5521 cggccgggta cgacccactc gtcgatgagg gcaatgcgtg ggcagagcgg ctcgccgctg
     5581 ccggcgtcac cgtacggcat cactgccacc cggggctcgt gcacggattt ctctcgcttg
     5641 ccggaatcgt gcgcgccgcc aagtcggcga ccgaccagat ctgcgtagaa atcgtcgagc
     5701 tcctttcaga gtgatacgct cggcccgtgc acgttctcat cgcggtcgct gatgtcggcg
     5761 ccggggtaca gctcgaagag gcactgacgc aggccggctt cgcagcgcgt tgggacggca
     5821 accaggcgga tggtccgcgc ggcgcgtcgc aacaggaggt cgtgctgatc gatgccgatc
     5881 gcctgggcga gaacctctgc tcggtgaccg acgcgtggcg cgatcacttg tcggtgcctg
     5941 gcgttgtcgc gctcggcacg tcggcgacgg cccgcgagca agcgccgatc gcgcgcgtca
     6001 cgctgctgtc gccatccgcg cagacgtcga cgatcgtaaa agcgatccgc gaagccgcga
     6061 agctgcggct ctcggtgggc atgcgctggc cagtgctgcg tgcggccctc aagttgccgc
     6121 cggccgcgaa tacacccgaa gcatggcccg cagtggtctt gcactcgcgc aatgcagatc
     6181 tcgaggtcgc gcg
//
LOCUS       DASUFX010000793         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1796010, whole genome shotgun sequence.
ACCESSION   DASUFX010000793 DASUFX010000000
VERSION     DASUFX010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796010"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1360
                     /locus_tag="VFV99_32025"
     CDS             215..1360
                     /locus_tag="VFV99_32025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064063.1"
                     /translation="MQIASVMADEVKPDPAREAPPIGKHNVARDDELVNLKLRRTPLK
                     VRALTAAGVVFLAIFFLIKLNPDRRFAGNSDTPKTVTVADLAQGHVDADAFVSVAAEP
                     MMSHAIRAQTAKNSVGLRVVPVRGSSEKLWIVLPGDGWVDPSKGAYTGRVRPLGDLPF
                     SGVLEDFLAAHPRPLFATAAAVRAGFGSSKLTTVAGDEISVGDNDRVGFDVVEPNAAV
                     IVGTYNERLPNVAAWTQAFIDAGITGFANGRDDRERVFFDVTMPNAVASIATKLEAAG
                     LWAARVDPVTHHHETTWRALKTSGPAGFTIGGLTIPDGQLDLIGLYVVRDIPSGAYAV
                     IVGEKPADYWYVLPVAILVGLIALVFAWALVRAIKRDFLSAKVAAAS"
     gene            1373..3784
                     /locus_tag="VFV99_32030"
     CDS             1373..3784
                     /locus_tag="VFV99_32030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5064064.1"
                     /translation="MADKPGEGPDAIPTVQAKVGGDAEPPLGTMQMPRGGVPGSPPAM
                     HPEGTSSPGGSGAPGPLMPTMVPSTQSPMPANAPPVMPQEGRYTINHEIARGGMGRVV
                     DATDTVLGRTVALKEALSLDPDALRRFQRETRITARLEHPSIVPVHDAGVSISGQPFY
                     VMRKVGGRPLEELVAHRESLNTRLALVPHVVAAAQAIAHAHTRGIVHRDIKPSNILVG
                     DLGETIVIDWGLAKGIDEADEPDSPIQRVIENDDAIKTRAGIVFGTPGFMAPEQLRGK
                     PVDERCDVYALGATLYHLLARRPPHHAKGADEMMKAAVKGSPTPLAQIVDGVPPELIT
                     IVDKALAHDAAHRYQDAGALAEDLQRFLTGQLVASHYYTPRERVMRFIRQHRNSVLIA
                     TGAVIALIVLAAVFIQQIREERDIAAEQRQLAVREKQRAEKQREEVIEKSRELVLNNA
                     RHSAADDPTRAVAMVKPLVDASHWRAARDVGAAARVHGIAFSMSASPHTLTLELSRDG
                     DSALAAGDDGVVRIYDLAKRESRVIADMRSAVMARYADGERKIVLFQGNRLSILDAAT
                     GDRRDVTAPTAIAQLEVSGPIAYWVDPANALWKLDLAGTAPSKIDVSEPVNAVEPSPD
                     GRWIALAGRDHLLLADRTNSTLPPEIVTIGHTKTMSWSADSEHLIVLINEEVIDVNMV
                     PAPQVFRRITVGQRFSAAYSGGRIYSAGPTGVGVVEQAGTKLRSAGPEYTVGVHEGRN
                     RVVVAAKPQGEILVLSDYGDHTLRCPVPISIVATSARGPWIVAASDNRLLLWNLDAIE
                     PRVLT"
     gene            3884..4798
                     /locus_tag="VFV99_32035"
     CDS             3884..4798
                     /locus_tag="VFV99_32035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064065.1"
                     /translation="MQLGLLDAIHSVASAPGGDEAVIIDSTRRAYRVAGLGQPQLLDG
                     DISAATFIDDRRLVVAGTGGLRIEDTQQHTKLALYAHDAAAKTIAATTADGGWVAAAF
                     EDGVLWRKHLSTNAAGELQVGPLTGGLPLAITDDGTTLFAIGGELRAWRPDGKVDVIA
                     KPITPLLALATVDQTHVLALAEAGVHLVELDHAASLPSPTVLLAKSAALARSGGLLAA
                     PTVTGGVEVIDPLVDWRWALATPQKGQAPFSVVDIAPDGSRVLGGSATEVFVWTLDLP
                     ADTDATKAWLANQTNATADNPSGPLGWN"
     gene            4820..>5665
                     /gene="cysS"
                     /locus_tag="VFV99_32040"
     CDS             4820..>5665
                     /gene="cysS"
                     /locus_tag="VFV99_32040"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5064066.1"
                     /translation="MTPFRLYDSMRGQKVPFEPLQPGKVGVYVCGPTTYAPAHIGHAY
                     SAIAFDTIRRSLEFLGWDVFYVRNVTDIDDKIINEANKTGQDPFAMSARFAADYNRDM
                     ARFNVSAPSIEPKVTGHIPQIIAIVERLVANGHAYVVDGDVYYSVESFPPYGKLSGQT
                     LDKLMDGARIDVDERKKNPADFALWKSAKPKEPFWESPWGKGRPGWHIECSAMTVTHL
                     GATFDIHAGGKDLIFPHHENEIAQSQGDTGVGTFARYWLHNGFLNFEGVKMSKSLGNV
                     FNCDQI"
BASE COUNT          964 a         1975 c         1846 g          880 t
ORIGIN      
        1 ggcttcgtag cctgcgtgac ttcgccttcg agccacacga ggccaagcgc atcgactgcg
       61 cgcgcggcaa gacgcaccac gtccgcgaca ctgaacggtc gctcgcgact gctcacccca
      121 tcgcgcggac ccgagacagg cacatccaac ggtaaccaga gcgcatcttc gtcgcaacca
      181 ccctgggtgg cgtggtgtcg ttatgaacgg taggatgcaa atcgcctccg ttatggccga
      241 cgaagtcaaa cccgaccccg ctagggaggc gcccccgatc ggcaagcaca acgtcgcccg
      301 cgacgacgag ctggtgaatc tcaagttgcg ccggacgccc ctcaaggtcc gcgcgcttac
      361 cgccgccggc gtcgtcttcc tcgcgatctt cttcctgatc aagctgaacc cggatcgccg
      421 gttcgccggc aacagtgaca cccccaagac cgtcaccgtc gcggaccttg cacagggcca
      481 tgtcgacgcg gatgcgtttg tatcggtagc cgccgagccc atgatgtcgc acgcgattcg
      541 cgcgcagacc gcgaagaaca gcgtcgggct gcgcgtggtg ccggtccgcg gctcgagcga
      601 gaagctctgg atcgtgctgc ccggcgatgg ctgggtcgac ccgtcgaagg gcgcgtacac
      661 cggccgtgtg cgtccgctcg gtgatctgcc gttttccggc gtgctcgaag acttccttgc
      721 tgcgcatccg aggccgctgt ttgcgacagc tgctgccgtc cgcgccggct ttggctcgtc
      781 gaagctgacc acggtcgccg gcgacgagat cagcgtcggc gataacgacc gcgttggctt
      841 cgacgtcgtc gagccgaatg ccgcggtcat cgtcggcacg tacaacgagc ggctgccgaa
      901 cgtggccgcc tggacgcagg cgttcatcga tgccggcatc accggcttcg ctaacggtcg
      961 tgacgatcgc gagcgcgtgt tcttcgacgt cacgatgccg aacgccgtcg cgagcatcgc
     1021 gacgaagctc gaagcagccg ggctgtgggc cgcgcgcgtc gatccggtca cgcatcacca
     1081 cgagacgacc tggcgcgccc tcaagacgtc gggcccggcg ggcttcacga tcggcgggct
     1141 cacgattccc gacggccagc tcgatctcat cgggctctac gtcgtccgcg atattccgag
     1201 cggcgcatac gcggtgatcg tcggcgagaa gccggcggac tactggtacg tgctaccggt
     1261 cgcgatcctc gtcgggctca tcgcgctcgt gttcgcgtgg gcgcttgtcc gcgcgatcaa
     1321 gcgcgatttc ctgtcggcca aagtcgcggc agcttcgtga catactctcg cggtggcgga
     1381 taagcctggc gaagggccag acgcgatccc gaccgtccaa gcgaaggtcg gcggtgatgc
     1441 cgagccacca ttaggcacga tgcagatgcc acgcggcggc gtgccgggaa gcccgcccgc
     1501 gatgcacccc gagggtactt cctcgcctgg cggctcgggg gcgcccgggc cgctgatgcc
     1561 gacgatggtg ccgtcgacgc aatcgccgat gcccgccaac gcgccgccgg tgatgccgca
     1621 ggaaggccgc tacacgatca accacgagat cgcgcgcggc ggcatgggcc gtgtcgtcga
     1681 tgcgaccgac accgtgctcg gccgcaccgt cgcgctcaag gaagcgctgt ccctcgatcc
     1741 ggacgcgctg cgccgcttcc agcgcgagac gcgaatcacc gcgcgcctcg agcatccgtc
     1801 gatcgttccg gttcacgacg ccggagtctc gatcagcggt cagccgtttt atgtcatgcg
     1861 caaggtcggc ggccgtccgc tcgaagagct cgtcgcacac cgcgaatcgc tcaacacacg
     1921 cctcgcactc gtcccgcacg tcgtcgcagc ggcgcaggcg atcgcacacg cacacacgcg
     1981 cggcatcgtg catcgcgata tcaagccatc gaacatcctc gtcggcgatc tcggcgagac
     2041 gatcgttatc gactggggcc tcgccaaggg catcgacgag gccgacgagc ccgactcgcc
     2101 gatccagcgc gtgatcgaaa acgacgacgc gatcaagacg cgcgccggca tcgtgttcgg
     2161 cacgccgggc ttcatggcgc ccgaacagct gcgtggcaaa cccgtcgacg aacgctgcga
     2221 tgtctacgcg ctcggcgcga cgctgtatca cctgctcgct cggcggccgc cacatcacgc
     2281 gaagggcgcc gacgagatga tgaaggcggc ggtcaagggc tcgccgacac cgctcgccca
     2341 gatcgtcgac ggtgtgccac ccgagctgat cacgatcgtc gacaaggcgc tcgcccacga
     2401 cgcagcccac cggtaccagg acgccggcgc gctcgccgaa gatctgcagc gtttcctcac
     2461 cggtcagctc gtcgcatcgc actactacac gccgcgcgag cgcgtgatgc ggttcatccg
     2521 ccagcatcgc aacagcgtgc tgatcgcgac cggcgcggtg atcgcgctga tcgtgctcgc
     2581 ggcggtgttc atccagcaga tccgcgagga gcgcgacatc gcggccgagc agcgccaact
     2641 cgcggtgcgc gagaagcagc gcgccgagaa gcagcgcgaa gaagtgatcg agaagtcgcg
     2701 cgagctcgtg ctcaacaacg cgcgccactc ggccgctgac gatccgacgc gcgcagtcgc
     2761 gatggtcaag ccgctcgtcg acgccagcca ttggcgtgcc gctcgcgatg tcggtgcggc
     2821 cgcgcgcgtg cacggcatcg cgttcagcat gtccgcgtca ccgcatacgc tcacgctcga
     2881 gctgtcgcgc gacggtgata gtgcacttgc cgccggtgac gacggcgtcg tgcggatcta
     2941 tgacctcgcg aagcgcgaga gccgcgtgat cgccgacatg aggagcgcgg tgatggcgcg
     3001 ctacgccgac ggcgagcgga agatcgtgct gtttcaaggc aaccgactct cgatcctcga
     3061 cgctgcgacc ggtgaccgcc gcgacgtcac cgcgccgacc gcgatcgcgc agctcgaagt
     3121 atcgggcccg atcgcctact gggtcgatcc tgcaaacgcg ctctggaagc tcgaccttgc
     3181 cggcaccgcg ccgtcgaaga tcgacgtctc cgagccggtg aacgcggtgg agccctcgcc
     3241 cgatggccgc tggatcgcgc tcgcgggcag ggaccatctg ctgctcgcgg atcgcacgaa
     3301 ttcgacgctg ccgcccgaga tcgtgacgat cggccacacg aagacgatgt cgtggtcggc
     3361 ggacagcgag cacctgatcg tgctgatcaa cgaagaagtc atcgacgtca acatggtccc
     3421 ggcgccccag gtctttcgcc ggatcacggt cgggcagcgc ttctcggcgg cgtacagcgg
     3481 cggccgcatt tacagcgcag ggccgaccgg tgtcggcgtc gtcgagcagg caggtacgaa
     3541 gctgcgctcg gccggtcctg aatacacggt cggcgtgcac gaaggccgca accgcgtcgt
     3601 cgtggcggcg aagccgcagg gcgagatcct cgtgctctcg gactacggcg accacacgct
     3661 gcgctgcccg gtgccgatct cgatcgtcgc gacgtccgcg cgcggaccgt ggatcgtcgc
     3721 cgcgagcgac aaccggctgc tgctgtggaa cctcgatgcg atcgagcccc gcgtgctcac
     3781 ctagcgctcg ccgagcagcg cgcgtctcgt caccaacgac cacgcgatcg tcacgtactt
     3841 cgacgagcca gccgagtgga tcgacttgcg caagaacacc accgtgcagc tcggcctcct
     3901 cgacgcgatc cacagcgtcg ccagtgcgcc tgggggcgat gaagccgtca tcatcgacag
     3961 cacgcggcgc gcgtaccgcg tcgccggcct cggtcaaccg cagctgctcg acggtgacat
     4021 cagcgccgcg acgttcatcg acgatcgccg gctcgtggtc gccggcaccg gcggtctgcg
     4081 catcgaggac acccagcagc acacgaagct cgcgctctac gcgcacgatg cggccgccaa
     4141 gaccatcgcc gccacgaccg ccgatggtgg ttgggtcgcg gctgcgttcg aagatggcgt
     4201 gctgtggcgc aagcacctgt cgacgaatgc cgccggcgag ctgcaggtcg ggcccctcac
     4261 cggaggtctg ccgctcgcga tcacggacga cggcacgacg ttgttcgcaa tcggtggcga
     4321 gctgcgcgcg tggcgtcccg acgggaaggt cgatgtgatc gcgaagccga tcacgccgct
     4381 cttggcgctc gcgaccgtgg atcaaactca cgtgctcgcg ctcgcggaag ccggtgtcca
     4441 cctcgtcgag ctcgatcacg cggcgtcgct cccgtcgcca accgtgctgc tcgcaaagtc
     4501 cgccgcgctc gctcgctccg gtggcctgct cgccgcgccg accgttaccg gcggggtcga
     4561 ggtgatcgat ccgctcgtcg actggcgctg ggcgctcgcc accccgcaga aagggcaggc
     4621 cccgttctcg gtggtcgata tcgctcccga tggctcgcgc gtgctgggcg gaagcgcgac
     4681 cgaagtgttc gtgtggacgc tcgacctgcc cgcggacacc gacgcgacga aggcctggct
     4741 cgcgaaccag acgaacgcga ccgctgacaa tccgtcgggg ccgctcggct ggaactagcg
     4801 ccatgctaaa accggcggag tgactccctt ccgcctctac gactcgatgc gcggccagaa
     4861 agtgccgttc gaacccctgc agcccggcaa ggtcggtgtc tacgtctgcg gtccgacgac
     4921 ctacgccccc gcgcacatcg gtcatgccta ctccgcgatc gcgttcgaca cgatccggcg
     4981 cagcctcgag ttcctcggct gggacgtctt ctacgttcgc aacgtcaccg atatcgacga
     5041 caagatcatc aacgaggcga acaagaccgg ccaggatccg ttcgcgatgt cggcgcggtt
     5101 cgcggccgat tacaatcgcg acatggcgcg cttcaacgtg agcgcaccgt cgatcgagcc
     5161 gaaggtcacc ggtcacatcc cgcagatcat cgcgatcgtc gagcggctgg tcgcgaacgg
     5221 ccacgcctac gtcgtcgacg gcgacgtcta ctactcggtc gagtcgttcc ccccgtacgg
     5281 caagctgtcg ggccagacgc tcgacaagct catggacggc gcgcgcatcg acgtcgacga
     5341 gcgcaagaag aatccggccg acttcgcgct gtggaaatcg gcgaagccga aagagccgtt
     5401 ctgggagtcg ccgtggggca agggtcgccc cggctggcac atcgagtgct cggcgatgac
     5461 ggtcacgcac ctcggtgcga cgttcgacat ccacgccggc ggcaaggatc tgatctttcc
     5521 gcaccacgag aacgagatcg cgcagtcgca aggcgacacc ggcgtgggca cgttcgcgcg
     5581 ctactggctg cacaacggct ttctgaactt cgaaggcgtg aagatgtcga agtcgctcgg
     5641 caacgtcttc aactgcgatc agatc
//
LOCUS       DASUFX010000794        24633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_268492,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000794 DASUFX010000000
VERSION     DASUFX010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24633
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268492"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFV99_32045"
     CDS             complement(<1..533)
                     /locus_tag="VFV99_32045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064067.1"
                     /translation="MLHRWHFFRAGAVDQVTLRDGFDLLALPDLDQKLWVALAMPTNG
                     VDVDPLTLQLMDVNSDGRIRVHDVLTAVADIKRIFKNPTEVLTSADSVELEAIADKPV
                     LAAAKRMLADLGKKDAIAITIEDTIAVTKAFADTVLNGDGIIIPASADAGPVRAAIED
                     AIATVGSVNDRSGKPGI"
     gene            complement(651..1118)
                     /locus_tag="VFV99_32050"
     CDS             complement(651..1118)
                     /locus_tag="VFV99_32050"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HEX5064068.1"
                     /translation="MTLDPTMLRLSFDLIIDRRPDLTVRFYEILFERYPALAPMFRRD
                     RSAQAKMLAGAIAAVLDHLEDAPWLVSTLGQLGAKHVEYGVTHAMYDQVGDALLATLA
                     EVAAESWTPAVAEQWTLAFGAIASMMQAGAAVVMDTRASQPRMHRTTLPETEL"
     gene            complement(1229..2473)
                     /locus_tag="VFV99_32055"
     CDS             complement(1229..2473)
                     /locus_tag="VFV99_32055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5064069.1"
                     /translation="MQSALRTAAAGLIGNVLEWFDYAVFGYFSAEIGKQFWPATMAKD
                     DRDLLTFAIFWVGFAARPIGGLVLGIVGDRIGRRALLTLSIALMGGATLVIGCLPTYH
                     SIGIAAPILLGLMRIVQGFSLGGEFTGSMVYTTELASPLVRGIIASSTAAGTTLGFLL
                     GSLTAWLVRLSLGDAAAAEWGWRIPFLASILLCIIGWLLRRGIHESEQGVKAAAERAP
                     IVKSLIADWKPIVQTFGIVAMTNAAYYLAFTFAVERRKAESTTFQLINTISLAVVLLA
                     KILGGWLSDRLGRRRMMIALSIVLMLAIYTSQRFMLEGEPALFMVGQLMLGIPLAMAL
                     GLQGAMVVEIFPLRTRVTSMSFAYSVTLALAGGLMPIIATWLIDKAHSPMAPAFFVMA
                     YGIIGVPIMLGMRETNQRSLDD"
     gene            complement(2483..2893)
                     /locus_tag="VFV99_32060"
     CDS             complement(2483..2893)
                     /locus_tag="VFV99_32060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064070.1"
                     /translation="MAAVIPVRTFVSETPARTASERNSSGVIIALIVSVSVDICFACR
                     SPTVDPVYLVETREQSEQVAPAEAYCLQNREENCSRIISSLIHRHPAVQSFASVFRLR
                     FDEIVEKRGPSSFASLLCTERAVVAASSVRATTR"
     gene            2811..5006
                     /locus_tag="VFV99_32065"
     CDS             2811..5006
                     /locus_tag="VFV99_32065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5064071.1"
                     /translation="MTPELFRSLAVRAGVSETKVRTGMTAATGAIMEGLARHAHNSSV
                     MDDTVQLVDRTPEVSDPLVAVEEDDRMRSASNQMLGLATNDTSGVTKRLASSLGVGTG
                     VASTLLAAASSIILNGLHRFSRARGGMDSTALSATLLAEAPGFFAAMPGPVPSSWRER
                     ERRIERPAARSHERRWLWLLALIPIVAIGAWLINRHERGERRNATPMGKVTEPDRMAP
                     QQRAITPPAPEPKSVVPEEPAPMPERQTMPDQTQTEPTPGEAQLQPAQWQLPLPSDQD
                     QQVSGAESPAIGVDAQQAQESDQVSGAESQVTGDAQQAQESSENQQAMGPEAQQEQQT
                     NQGESTESQQEQQRKQDQQAMGPESQQEQQRDQDQQQGTTSQPGELNLSQPTTGTEPQ
                     QEQQRKQEQQATAPEAEQGQESNQEPSAQERMVPVPGMQSKQEQQLNQEQGTESQQDQ
                     QRKQDQQATAPEAEQGQESNQEPSATGTESQQDQQREQEQQTTPQAQQEQQRPIPVPG
                     MESQQVPQPNQSQQDQQRKQEQQAQGEQQPQQPQQPPVEINPGTPPSEQPPVEINPGT
                     PAGQPQEESKPPVTQPEQPSAGTQAQTTQPSAPEGDVAAAIEAQIESPNPPEEGVTLD
                     ELKFDSGRAQIKENASASEQLSRVAEILDANPNVRIQINGYTDLTGNKDANMKLSQKR
                     AEAAKEALMQRGIDGSRIEAKGRGGEEPMEETPVQSDANRRVTVLVVSR"
     gene            complement(5034..7367)
                     /locus_tag="VFV99_32070"
     CDS             complement(5034..7367)
                     /locus_tag="VFV99_32070"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5064072.1"
                     /translation="MTAPDRIQRTLARVVRYRIAIIVLYALLVPAAALLATSIPSEEG
                     IGRLIVASDPDVRATREYHEIFPEPQLALLVFESPDPWSPSSLERVDRARAALAAVPR
                     VTAYSVVDMLRRARPGADPETLRALANGTPTFRRQGLAGDTFLAIVVNLAVVGPTDRD
                     TALDAIDVALARSGAGAVRKIGTPYVNAWLERAAGSTMARSLVVLGVLLVSIVLFLFR
                     SLRALIAIVLALGSTVALALGAGSLLGFEFTIVSALVPLTVMVTTLATLTYLHSRYVD
                     QPEGVPLAEHQLFALRTKLLPVTASTIAAATGFAALAVSKIQPIRQMGIWTALGLLIS
                     WIVAYTLFPALQRVLRTPTGRQIQVRTKAYDRVSRALPELTYRHRKAFVAGALAFCVA
                     GALAVTGVPGIFSGLPVAVDVLTNIDPASRVARDIRWFRAHASDLNVAHVWIHLPHPA
                     ATEPEVLHAIDRYATALDATTNVTGVTGPTTPLRMRSYFAGRGESLPRDPAAFASAVQ
                     DVEQLLLSEPELRAFIDASGLADLQLTVLFRDGHAKGYNALTARAATAWTEALATSPS
                     LAGATMRVVGTALLDAKISESLVPTLAESLVLTVVVILMVFLIVFRSGTERLLAMIPS
                     LFALLVTFLGLRLFGGTLNVATIIIATTVLGTTENDQMHFFHHLHEGPRASLEARLHH
                     TLQIAGRAIVFATVINAIGFLGLAPSSFPPLRQFGLMTASAFVLALLADFLVLPAALW
                     LASGERPASVAPPAAPPAVVPVTKAGGFEAAPAGVKS"
     gene            7496..8326
                     /locus_tag="VFV99_32075"
     CDS             7496..8326
                     /locus_tag="VFV99_32075"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5064073.1"
                     /translation="MRRSIQLALVAVLAVGAIAVAAAPGKLLLVICSPGSPGSTEEAQ
                     PRIDAFARALSAKAGTTITAVYEPTEAGGVKQLGGAAVGVVSLPFFLVHEKDLGMHAR
                     LVAVQEGRPALESWVLVAQKGRIHKAEELAGFTIASSAAFAPGFVRAATAGLGALPAN
                     VKLQEMTAVLSSLRRAAAGEPIAVLLDGPQAGALKSLAFADKLDVVTRSPAWPAGIVV
                     SLDARMPAKAWAPIQTALTGLAADRAAAQTLAAIQIAKFVALDEKSLASARQAYGRKP
                     "
     gene            8323..9111
                     /locus_tag="VFV99_32080"
     CDS             8323..9111
                     /locus_tag="VFV99_32080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064074.1"
                     /translation="MKSAVLASVLFCAACSSALKEPSPIATYAPGHADGRTANELVAA
                     ANAAWAHRGEAGKAEAAQGLYLDAAVVDPHRFDAIAGAMRAISYRIENEQHVAREKLA
                     KISVELGQLCQRRAPKQPECDYRLAIALGQYARENTASAKDAVNRMVGLLHRAIAAAP
                     RLDAGGPHRVLAYLLLRAPGWPLGPGDEEAGLVQARAAVQLAPNAENELVLGEALAKN
                     GHPNEARAAYAKAAELATAAHEAGEPEAARWLAQARAGLAKNSG"
     gene            complement(9108..10148)
                     /locus_tag="VFV99_32085"
     CDS             complement(9108..10148)
                     /locus_tag="VFV99_32085"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5064075.1"
                     /translation="MKPWSYYQHWLDVVSRSFALCIPQLGPPFRDQVALAYLLFRVID
                     TIEDAPFTDKAEQQKQFERLRAFLRAMPSRQEVDTFAAAFPTNLTAGERSLLGETYDL
                     LAEGHALPWPARAASFRAMDRMAMGMAAYTRRPTPLRLVDVEDVARYCCFVAGVVGEM
                     LTQLWTLERNAPAPHTSLAYHFGLFLQKVNILKDQHDDEAAGRFFVPNRTDLIASLRK
                     DAQGALAYIQAIPEGDQYRIFCAWSLMLGAVTIAQLDEPKRSRRDEVGRLLADTAAIV
                     DDNAALAKQFAELMPALPEIVEHSPIAKPESFEWFRTTLSARLTDAELHDLGIGGGRT
                     TGRTIVAARSTR"
     gene            complement(10145..12100)
                     /gene="shc"
                     /locus_tag="VFV99_32090"
     CDS             complement(10145..12100)
                     /gene="shc"
                     /locus_tag="VFV99_32090"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX5064076.1"
                     /translation="MVEPEIDDARAGADQVIARAHDRFRREQFADGYWWAELESNATM
                     DAEYILMTHVIGERDDAIWRAVAQDIRSYQRADGSWALYAGAPGDLSTTIECYFALKL
                     AGDTGPHLDRAREFAMARGGIAGARVFTRIWLAICGEWSWDDLPTMPLELMLLPDRAP
                     MSIYRFSSWARATIVPLLLVMNDRPARPVPPAAGLAELRIATATVAPTPRNPIDRVFL
                     AIDAVLRQYQRLPWHPLRERARKAAERWIVEHQEADGSWGGIQPPTVYSLMALHTLGY
                     RSDHPVIRKGIDGMHDKWMIRRADGSHRVQACLSPVWDTALSVWALVESEVAPADPAI
                     QTATRWLRKEEVRVPGDWCVWVPNVEPSGWAFEFENDLYPDIDDTAVVLLALHRVGAL
                     DDETQKRAVAWLLAMQSENGGWAAFDKDNTARLPAMIPFADFGEMLDPPSADVTAHVV
                     ETLAALGFDRGHPAIVRALDYLYAEQEHEGSWFGRWGVNHVYGTGAVLPALAAIGEPM
                     DSISVRRAIRWLVDHQNRDGGFGEGCESYIDPKLRGRGVSTPSQTAWALLGFVAADRA
                     DSTVAHRAARYLLDNQRADGGWDEHAYTGCGFPGYGVGEQRGARIRQGRELRSAFLLR
                     YHLYRDCFPLLALGRFRAAVRRRGKVV"
     gene            complement(12104..13099)
                     /locus_tag="VFV99_32095"
     CDS             complement(12104..13099)
                     /locus_tag="VFV99_32095"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5064077.1"
                     /translation="MRGALVKALVTGASGFIGGAVARTLLAKGIDVRVLLRPGTTPNV
                     PDPAAVDIVRGDLRNASSVHAAAAGCAAVFHVGALYSFAADRTALHETNVGGTRNVIA
                     AARAAGARLVYTSSISTIGGMREGAIPDEGQDAGHIAPGPYKESKWRAERLVRDEAVR
                     GVDAVIVNPTFPIGWGDVKPTPTGALIRDFLHGRLPAYVDTGMNVVDVDDVAEGHWLA
                     LERGRRGERYILGNANLTMRELLELLAQVSGRKAPRLRLPYALALGLAYADKFLFRRH
                     ARIPLEGVRTAHEIRFASSARAIRELGLPQTPVRTTLEKAVRWFQLQPAAAQERS"
     gene            complement(13081..14088)
                     /locus_tag="VFV99_32100"
     CDS             complement(13081..14088)
                     /locus_tag="VFV99_32100"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064078.1"
                     /translation="MTDTRLMQLVQQAFDYRGYVTVVRHDGSSLVGFVYQQDVAEIAM
                     FDEDATQRVTVPLRDIADISITGDDSAARAQRRWERRRDSLEARETSAWGEWEQRPTL
                     ILVALPIELRGIASVLRSKVRGTSASAMLGEHRVIARAVGVGGGAAHVVAADRPSLVI
                     NCGFAGALEKSLGAGDLVLASSVRDESGESVMARESVLRIARRALAYTQVAEGELLCA
                     TRIAATREDKRILARPGRLAVDLESWASARAAERAGIPWLALRVVLDPLEIDLPRFAS
                     EAREHYVPSVVRHTLRGPRAVLDLARLGTRTAIAFRSLRRAMQQLMPVLGELGACEVH
                     S"
     gene            complement(14085..15101)
                     /gene="hpnH"
                     /locus_tag="VFV99_32105"
     CDS             complement(14085..15101)
                     /gene="hpnH"
                     /locus_tag="VFV99_32105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX5064079.1"
                     /translation="MGIPLAQMYAVARYVVNQRLAGRERYPLVLMLEPLFRCNLSCTG
                     CGKIQYPQSLLKKQLSVAECLAASDECGAPIVSIAGGEPLLHPDVPQIVRGLSKRGRF
                     VYLCTNALLLQQRLDEFKADKHLSFSVHMDGGRVDHDRSVAREGVYDTAVAAIREALR
                     RGFRVTTNTTLFDGASPERVRKFFDDMMELGVEGMMISPGYSYEKAPDQEHFLKRERS
                     RRLFSQIFDAPHKRWRFNQSPLFLRFLMGKLDFECTPWGNPTYNIFGWQRPCYLLQDG
                     YANTFRELMEKTDWSKYGHASGNPRCQDCMVHCGFEPTAVNSTFTSTKGLARTVASMV
                     TGRP"
     gene            15423..16760
                     /locus_tag="VFV99_32110"
     CDS             15423..16760
                     /locus_tag="VFV99_32110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064080.1"
                     /translation="MAHADPAPTPDVAGAPLPGNESGQTWNDEGEPPEGSLPRNLGRA
                     VLFIPKSLAWLLLQPVRGAVYVYDRYDLENRYYETFYNESRTFGIVPVIEYATGFSIM
                     VGGRLIAKDVFGAQENYTMMGAWGGEYQSRAEAWLDSGTRFDPVTVTVGGNFDRFARL
                     AFYGIGNNSNRLDMPPPTLINPLVNDTAVQTFYRYQEMRAALTADWRLIDDFHLLAHG
                     AVTGIVTDESTKDPSIETVYNPMDLVGFDEMTTHVYGQLEARWDTRRNAVPTWETTQY
                     STGYLVSGYVGGVQGLGDAHNFAHYGVDLQAFVHLALGPRMLWFRFLGEGVTGDLNEI
                     PFAELPYLGGDFLRGYDFARFRDRVSALAVAQYTWDLSRFTNAYLFVDAGRVYHSIED
                     ATLDDMNVGFGGGIELQSSKSFLLAATLASSIDGGVFVSAVLAPYHSQVPRWR"
     gene            16757..18412
                     /locus_tag="VFV99_32115"
     CDS             16757..18412
                     /locus_tag="VFV99_32115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064081.1"
                     /translation="MSSLRYMAVIVVATTSCKQSAEVPRVRYANAAPVTVVNDRRAVP
                     TRPHVSRQLLDLDFYDRSVEDPLMNTLSLPQKQRARGVNAVDEVPNSTWFTNRIGVRD
                     MSPQEIANGPLGDEGPEPHKPWTVHSTKPGGTEVGFIITDARGVKYGLGFDTPEWPEM
                     ETGNAVVVNRLLWAFGYNVPDERIAYIRPEDLVPAANAQVKDSQGRRLRQLDRKELDR
                     QLALVSHAPDGRIRVLASRWLSGESLGGTNPTGVRKGDPNDVIPHEDRRDLRGQYPVF
                     AWLEHLDLVRGNFLDMLIAPHDPAHRYVVHYLLDFGRSLGAIAVIDKNLRANHRYLVD
                     WSDPIWTFGTEPRSWGKHAAPLLTGVSPTFVATGFDPGAWKPNLPYQPFDSADRFDMF
                     WGTKILGRFTPEQIRAAVEAGRFSDPRTVDYLTDTLIRRQQATMAYWYVRVNPLDKFT
                     VGPNGLCFEDLAMMGRLVPGAPTRYELASYDRDARPLGTVTINAAQGGATCTGSVKLS
                     NVNGGYTIVKITTLRPVANGSTIVHLARAPKSAEWRVIGVMRV"
     gene            18443..19582
                     /gene="hpnI"
                     /locus_tag="VFV99_32120"
     CDS             18443..19582
                     /gene="hpnI"
                     /locus_tag="VFV99_32120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEX5064082.1"
                     /translation="MSVVLFVMLVASWSFWIVSVSVARRVFTPRAGSPTWFPPVSILK
                     PVRGLDFEARQNFISHCTQRYPTYEILFGVTSGDEPVVPLIKELQRTYGTERVQLVIA
                     PNEALNPKAGLLDALAQRARYDVIVLTDSDVRLPPDGIARAIAPLADPSVGLVTLLYR
                     GDRVLSTAAAFEALGMEAEFLPSALVAREVLRAPFALGPGNAVRRDALERIGGFSAVS
                     NYLADDYQLGFQIGATGKQVEVGDVVISAVLGPTSLGVWWQREVRWARTIRMSRRMHY
                     PGLMFTFATPLALLATLAYGWWPALIGSLAVRWYVGAQMTRWMGDRALRRWLWLLPLR
                     DLISPAIWFAGLLGKRVSWRGRTFRVAPDGQLYEPPRDVKAARVT"
     gene            19582..20634
                     /locus_tag="VFV99_32125"
     CDS             19582..20634
                     /locus_tag="VFV99_32125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5064083.1"
                     /translation="MVEPRTRIRLRTLLGSVLGVGLLVGATVLAASYFTDLRTLLHVL
                     ANGQWPWIAGAVGAQVAYLFGYALFYRLGFAVVGVSSRTWSLVPVLFTGLFVNQLVPT
                     GAGAAAVFVDDAVRRGQSGARVTVGVVLVLVLDLLAVLPFVVWGIAFLVHRQLFASWQ
                     LFAAGAFAMYVVLLVVLLALSHAKQASVCRALTICYRVIHRVLAWIRVRGPSVDWPAR
                     TAAGFADAAAAIAANRGQLALAGVWGIAIHLLNAFGLWLFVRGFGADVPLGGLIAAYA
                     LGVVLVVIAVIPQFAPISQAFVTATFIGVGMATGPAVASTLAFRGLALGLPLVVGLPF
                     AYRFGRFRVDVLRGAT"
     gene            20631..21743
                     /locus_tag="VFV99_32130"
     CDS             20631..21743
                     /locus_tag="VFV99_32130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064084.1"
                     /translation="MIAVDFARFAIGTVVLYAATTVVFDVLHYALHRCAASRFRVLRR
                     VAAPHVVHHEFLGTSLRFDERLVWPNIWWHRVPEWTVQVAATACAAPFVPLSWVLCAA
                     ATHTVLFVITVVGRGKDGNHVSLTTVPSPRSWFVGPAYHSLHHAYPESYMSSFVKLFD
                     WVLGTGCHLRGKRIVMTGASGAFGEPLRQLLEQEGAAVTALRFGHDWTYDDYEGVDRA
                     LATADILVLAHGAKGPQAMQANCDSFVAFIERFLSRHPNALIPIEVWAVGSEIEAHPA
                     WGNPELQVYLESKRAYARHAWRYFRDRRFTYRHIVPAAFRSRMGPGLMSGRTAARTAM
                     FFIRRGFRYVPVTYTGIAFVNYWKFVFSRRDTSPTL"
     gene            21756..22385
                     /locus_tag="VFV99_32135"
     CDS             21756..22385
                     /locus_tag="VFV99_32135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HEX5064085.1"
                     /translation="MKLGLVLALALASCGGKSPPAAHSNVAPSPTAPGVLRAVADFDA
                     IGDPAARSRALFAEAARVLTHPRCVNCHPSGDSPHQRMAMEMHDPPVVRGPANNGVPG
                     MECTTCHQDRNQALTRVPGAPKWHLAPIEMAWVGKSPAYICNQIKDPKRNGGKTLAQI
                     VDHSAHDALVAWGWSPGADREPVPGTQEQFGALVEAWVKTGAECPEETR"
     gene            22382..22849
                     /locus_tag="VFV99_32140"
     CDS             22382..22849
                     /locus_tag="VFV99_32140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5064086.1"
                     /translation="MKIKINGVEQTLDIDPDMPLLWALRDVLGLTGTKYGCGEALCGA
                     CTVHLDGRAVRACVTPVRRAEGRSVTTIEGLSSDGSHPVQQAWLDLRVPQCGYCQVGQ
                     IMTAAALLEAKPKPTDAEIDQSMAGNICRCGTYTRIRSAIKKAAGMPDDGGDK"
     gene            22846..>24633
                     /locus_tag="VFV99_32145"
     CDS             22846..>24633
                     /locus_tag="VFV99_32145"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5064087.1"
                     /translation="MTPPIKLTRRSFIAGLGAGLAVGVMPRIAHAKEPTKEQGRGLDP
                     DVLVHIAIDGTTTIVCHRSEMGQGIRSSLPVLIGDELGADPARIVVRQAEGDKKYPDQ
                     NTDGSTSIRKQYDVLRQAGAAARMMLIAAAAKRWKVKPETCTTANHTVIHTPTKRALA
                     FAEVVDAAAKLPVPKRESIVLRPIGELKNVAAPTLPLLDGRGYVTGTAEYGADVKLPG
                     MVVAVIARPPVVGGKVAKYDATRALAVPGVKKVIEMPAAKPPWDFQPWGGIAVVADNT
                     WAALKGRAALEITWDHGPEASFNSSAYHDELLASVRGPGTKARDVGDIDAAFAKAAKT
                     VEAEYFVPHLSHLQMEPLVAVARITNGNVEVWSPTQGPQDNREDIAKILGIPEDKVRV
                     NVTLLGGAFGRKAKSDFPIEAVYIAKQLGDGVPVRVQWTREDDVKHGYYNAVNAQRLR
                     AALDDKGTVTAWHHKTAFTPIKGTFDDKVDTPSIDDLQQGVTDLALAAPNVRVEACKA
                     PLHTRVGWFRSVYNIFHAFAIGSFIDEIAHARGADPRDTWLDVIGPARKLSLADLGVA
                     RLRNYGETLDKHPVDAGRLRNVITRVTDMA"
BASE COUNT         4379 a         7962 c         8248 g         4044 t
ORIGIN      
        1 tcgataccgg gcttgccgct gcgatcgttg acgctgccga cggtcgcgat cgcatcttcg
       61 atcgctgcac gcaccgggcc ggcatccgcc gaggccggga tgatgatccc gtcgccgttg
      121 agcaccgtgt cggcaaacgc cttcgtcacc gcgatcgtgt cctcgatcgt gatagcgatc
      181 gcgtccttct tgccgagatc agcgagcatg cgcttcgcag cggcgagcac gggcttgtcc
      241 gcgatcgcct cgagctcgac cgagtcggcg gaggtcagca cctcggtcgg gttcttgaag
      301 atccgtttga tatcggcgac ggcggtcagc acgtcgtgga cgcggatgcg gccgtcgctg
      361 ttgacgtcca tcaactggag cgtcagggga tcgacatcga cgccgttggt tggcatcgca
      421 agcgcgaccc acaacttctg atccagatcg gggagcgcga gcaggtcgaa accgtcgcgc
      481 aacgtgacct gatcgaccgc acccgcacga aagaaatgcc accggtgaag catcgccggc
      541 agtctagacc cgcgagcttt gcgcgagggg gcgatccgtc tactagtttt tcgcgtccgc
      601 tgttacgctt gcggcttctt tggattcgtc acggcgatcc tccgtccggc ttagagctcg
      661 gtctcgggca gcgtcgtacg gtgcatgcgc ggttgcgagg cccgcgtgtc catcacgacc
      721 gcggcgccgg cctgcatcat gctggcgatc gcgccgaacg cgagcgtcca ctgctccgcg
      781 accgcgggcg tccacgactc ggcggcgacc tcggcgagcg tcgcgagtag cgcgtcgcca
      841 acttgatcgt acatggcatg tgtcacgccg tactcgacgt gcttggcgcc gagctggccg
      901 agtgtcgaca cgagccatgg tgcgtcctcg aggtgatcga gcacggccgc gatcgcaccc
      961 gcgagcatct tcgcctgcgc gctgcggtcg cgccggaaca ttggcgccag cgccggataa
     1021 cgctcgaaca agatctcgta gaaccggacc gtcaagtccg ggcggcgatc gatgatgagg
     1081 tcgaagctga gacggagcat ggtgggatcg agtgtcatgg cccatcccta agagcacgcc
     1141 gaatgccggc gccagccgcg taggttcgcg ggcttagcag cgcacgcagc gtgcgcgcac
     1201 gctgaccgca cgggaaagtg gtgccgcgtc agtcgtcgag cgaacgctga tttgtctcgc
     1261 gcatgccgag catgatcgga acgccgatga ttccgtacgc catcacgaag aacgccggcg
     1321 ccatcggtga gtgcgccttg tcgatgagcc acgtcgcgat gatcggcatc agcccgccgg
     1381 caagtgcgag cgtgacgctg tacgcgaagc tcatcgaggt cacgcgcgtg cgcagcggaa
     1441 agatctcgac caccatcgcg ccctgcagac cgagcgccat cgcgagcggg atgccgagca
     1501 tgagctgccc aaccatgaac agcgcgggct cgccctctag catgaagcgc tggctcgtat
     1561 agatcgcgag catcaatacg atggagagcg cgatcatcat gcgccggcgg ccgagacgat
     1621 cagacagcca gccgccgagg atcttcgcga ggagcaccac ggcgagcgag atcgtgttga
     1681 tcagctggaa cgtcgtgctt tctgccttgc gtcgttcgac agcgaacgtg aatgccaggt
     1741 agtacgccgc gtttgtcatc gccacgatgc cgaacgtctg cacgatcggc ttccagtccg
     1801 cgatgagcga cttgacgatc ggtgcgcgct cggcggcggc cttcacgcct tgctctgact
     1861 catgaatgcc acggcggagc agccagccga tgatgcagag caagatgctc gcgaggaacg
     1921 gaatccgcca gccccactcg gcggctgccg catcgccgag gctgagccgc acgagccacg
     1981 cggtcagcga gccgagcaga aagcccaacg tggtgccggc ggcggtcgag ctcgcgatga
     2041 tgccgcgcac cagcggcgag gcgagctcgg tcgtgtaaac catcgagcct gtaaactcgc
     2101 cgccgagtga gaagccttgg acgatgcgca tcagcccgag caggatcggg gcggcgatgc
     2161 cgatcgagtg ataggttggc aagcaaccga tcacgagcgt tgcgccgccc atcagtgcga
     2221 tcgacagcgt gagcaatgcg cggcggccaa tccgatcgcc gacaatgccg aggacgagac
     2281 cgccgatcgg tcgtgccgca aacccgaccc agaagatcgc gaaggtcagc agatcgcgat
     2341 cgtccttcgc catcgttgcc ggccagaatt gcttaccgat ttccgccgag aagtacccga
     2401 agacggcgta gtcgaaccac tcgaggacgt tgccgatcaa tcccgctgcc gcggttcgaa
     2461 gcgccgattg cacggcggca tactaccgcg tcgtagcgcg gacggatgac gctgcgacta
     2521 ccgcgcgctc cgtacagaga agcgaggcga acgatgaggg gccacgcttc tcgacgatct
     2581 cgtcgaagcg gagacggaac acggatgcga acgactgcac ggccgggtga cggtggatga
     2641 gcgaagagat gatccggctg cagttttctt ctcgattctg caagcaatac gcctcggcgg
     2701 gcgcgacttg ctccgattgc tcgcgcgtct cgacgaggta gaccggatcg acggttggtg
     2761 agcgacaggc aaagcaaatg tccacactca ccgaaacaat caacgcgatg atgacacccg
     2821 agctatttcg ctcgctcgcg gtccgagcag gtgtctccga aacgaaggtg cgaaccggga
     2881 tgaccgcagc caccggcgcg atcatggaag gtctcgcgcg gcatgcgcac aactcgagcg
     2941 taatggacga tacggttcag ctcgtcgatc gcacgcccga agtctccgat ccgctcgtcg
     3001 cggtagagga ggacgatcgc atgcgctcgg cgagcaacca gatgctcggc ctcgcgacga
     3061 acgacacgag tggcgtgacg aaacgactcg cgagctcgct cggcgtcggc accggagtcg
     3121 cgtcgactct gctcgcggca gcatcgtcga tcattttgaa cggattgcac cgcttcagcc
     3181 gcgcgcgtgg cggtatggat tcgaccgcgc tgtcggcgac gttgctcgcc gaggcgcccg
     3241 ggttcttcgc ggcgatgccg ggcccggtgc cgagcagctg gcgcgaacgc gagcgccgca
     3301 tcgagcggcc ggcggcgcga tcgcacgaac ggcgctggct atggttgctc gcgctgatcc
     3361 caattgtggc gatcggcgcg tggttgatca atcgccacga acgcggcgag cgccgcaacg
     3421 cgacgccgat gggcaaggtg accgagcccg atcgcatggc gccccagcag cgcgcgatca
     3481 cgccgcctgc gcccgagccg aagtctgtcg tgcccgagga acccgcgccg atgcccgagc
     3541 gtcagacgat gcccgaccag acgcaaacgg aaccgacgcc aggtgaggcg cagctgcaac
     3601 cggcgcagtg gcagctaccg ctaccctccg atcaggacca gcaagtcagt ggcgcggagt
     3661 ctccggcgat cggcgtcgac gcgcagcaag cccaagagtc ggatcaggtg agcggcgcgg
     3721 agtcgcaggt gacaggcgac gcgcagcaag cccaggagtc gagcgagaac cagcaggcaa
     3781 tgggccccga ggcgcagcaa gaacagcaaa cgaatcaggg cgagagtacg gagtcgcaac
     3841 aagagcagca gcggaaacag gaccagcagg cgatgggccc cgagtcgcag caagagcaac
     3901 agcgcgatca agatcagcag cagggcacca cgtcgcagcc aggagagctg aacctgtcgc
     3961 agccaacgac cggcaccgag ccacagcaag agcaacagcg gaagcaggag cagcaggcga
     4021 cggcacccga ggcggagcag ggccaagagt caaaccagga gccgtcggcg caagagcgga
     4081 tggttccggt gccgggtatg cagtcgaagc aggagcaaca gctgaatcag gagcagggga
     4141 ccgagtcgca gcaagaccaa cagcggaaac aggatcagca ggcgacggca cccgaggcgg
     4201 agcagggtca agagtcgaac caggagccct cggcgacggg caccgagtcg cagcaagacc
     4261 aacagcggga acaagaacag cagacgacgc cccaggcaca gcaagagcaa cagcgcccga
     4321 ttccggtccc gggcatggaa tctcagcagg tgccacagcc gaatcagtcg cagcaggacc
     4381 agcagcggaa acaggagcag caggcgcagg gcgagcagca accgcagcaa ccgcagcagc
     4441 cgcctgtcga gatcaatccg ggtacgccgc cgtcggagca accgcctgtc gagatcaatc
     4501 caggcacgcc cgctggtcag ccacaagagg agagcaagcc accggtcacg cagcccgagc
     4561 aaccgtccgc gggaacacag gcgcaaacga ctcagccatc tgcgcctgaa ggcgacgtcg
     4621 ccgcggcgat cgaggcgcag atcgagtccc cgaatccacc cgaagaaggg gtcacactcg
     4681 acgagctcaa gttcgattcg ggacgcgcac agatcaagga gaacgcgagc gcgagcgaac
     4741 aactgtcgcg cgtcgccgag attctcgacg cgaacccgaa cgtgcggatc cagatcaatg
     4801 gctataccga tctcacgggg aacaaggatg ccaacatgaa gctctcgcag aagcgcgccg
     4861 aggctgcgaa ggaggcgctg atgcagcgtg ggatcgatgg ctcgcgcatc gaggcgaagg
     4921 gccggggagg ggaagagccg atggaggaga cgccggtgca gtccgatgca aatcggcgcg
     4981 tcaccgttct cgtcgtgtcg cgctgagcgt ccatgcagaa cccggcagct cgttcatgac
     5041 ttgacgccag ccggcgcggc ttcgaagcca cccgccttgg tgaccggcac cacggcaggc
     5101 ggggcggcag gcggggcgac cgatgcaggg cgctcgcctg acgcgagcca cagcgctgct
     5161 ggcagcacga ggaagtcggc cagcagcgcg agcacgaatg cggatgcggt catcaggccg
     5221 aactggcgca gtggcgggaa gctcgatggt gcaaggccca agaatccgat cgcgttgatc
     5281 acggtcgcga acacgatcgc tcgccccgcg atctgcagcg tgtggtgaag gcgagcctcg
     5341 agtgatgcgc gagggccttc gtgcaagtgg tggaagaagt gcatctgatc gttctcggtc
     5401 gtgccgagca cggtcgtcgc gatgatgatc gtcgcgacat tcaacgtgcc gccgaacagg
     5461 cgcaggccga gaaacgtgac gagcaatgcg aacagcgatg ggatcatcgc gagaagccgt
     5521 tcggtgccgc tgcgaaacac gatgaggaac accatgagga tgacgacgac agtcagcacc
     5581 aggctctcgg cgagggtcgg tacgaggctc tcgctgatct tcgcgtcgag cagcgcggta
     5641 ccgaccacac gcatggttgc ccccgcaagt gagggcgagg tcgcaagcgc ctcggtccag
     5701 gcggtcgctg cccgcgccgt cagagcgttg tagcccttgg catgaccatc acggaacagc
     5761 accgtgagct gaaggtccgc gagcccactc gcatcgatga acgcgcgcag ctccggctcg
     5821 ctgagcagta gctgctcgac gtcctggacc gcgctcgcga atgcagcggg atcgcgagga
     5881 agcgactcgc cgcggccggc gaagtagctg cgcatgcgca gtggcgtcgt cggaccggtc
     5941 accccggtaa cgttggtggt ggcgtcgagc gccgtcgcgt agcgatcgat cgcgtgtagc
     6001 acctcgggct cggtcgcggc gggatgcggc agatgaatcc agacgtgcgc gacgttgaga
     6061 tcgctcgcgt gagcgcggaa ccaacggatg tcacgggcga ccctgctcgc cggatcgata
     6121 ttggtcagca cgtcaacagc caccgggaga ccgctgaata tccccggcac gccggtgacc
     6181 gcgagcgcgc cggcgacgca gaaagcgagc gcgcccgcga cgaacgcctt gcggtggcga
     6241 tatgtgagct ccggtaacgc gcgcgatacg cgatcgtagg ccttcgtgcg gacctggatc
     6301 tgacgaccgg tcggtgtacg cagcacgcgc tgtagcgcgg gaaacaacgt gtacgcgacg
     6361 atccacgaga tcagaaggcc gagcgcggtc cagattccca tctgccggat cggttggatc
     6421 ttcgagactg cgagcgccgc gaacccggtg gccgccgcga tcgtcgacgc agtcaccggc
     6481 aagagcttgg tgcgcagcgc gaacagctgg tgctccgcga gtggcacgcc ttcgggctgg
     6541 tcgacgtatc gcgagtgcag atacgtcagc gtcgcgagtg tcgtcaccat gacggtgagc
     6601 ggcacgagcg cggagacgat cgtgaactcg aacccgagca acgagccagc gcccagtgca
     6661 agtgcgactg tcgatccgag cgcgagcacg atcgcgatca gtgcgcgcag cgatcggaac
     6721 aagaacaaga cgatcgaaac gaggagtacg ccgaggacaa ctaacgagcg cgccatcgtc
     6781 gagccggcag cgcgttcgag ccatgcattg acgtagggcg tgccgatctt gcgcaccgcc
     6841 ccggcgccgg agcgtgcaag tgcgacgtcg atcgcatcga gcgccgtatc gcggtccgtc
     6901 ggcccgacga ccgcaaggtt gacaacgatc gcgagaaacg tgtcgccggc gagcccctga
     6961 cgccgaaatg tcggcgtgcc attcgcgagc gcgcgcaggg tttccgggtc ggcacccggt
     7021 cgcgcgcgcc gaagcatgtc gacgacggaa tatgcggtga cgcgggggac ggcggccaat
     7081 gctgcccgcg cgcgatcgac gcgctcgagg gagctcggcg accaaggatc cggcgactcg
     7141 aacaccaaca gcgcgagctg gggctcggga aaaatctcgt ggtactcgcg cgtggcacga
     7201 acatcagggt cgcttgcaac gatcaggcga ccgattcctt cttcgctcgg aatgctcgtc
     7261 gcgagcaacg cggcggccgg cacgagcagc gcgtacagga cgatgatggc gatccggtac
     7321 cgcacgacac gcgcgagcgt gcgctggatg cgatcaggtg cggtcaccgc cgtcgctcca
     7381 tgcgcagcgg tgagtcacgg cacgctccgg actgcgatcg cgagctctgt cgcgcgcgat
     7441 ccgcatgcgg tgtgctgcgc ttgactcgct cgcgatgtgg cgaacggttg tcattatgcg
     7501 cagatcgatt caactcgcgc ttgtcgccgt actcgcggtc ggagcgatcg cagtcgcagc
     7561 ggcgcccggc aagctgttgc ttgtcatctg ctcgcccggc tcgccgggct ccaccgagga
     7621 agctcaaccg aggatcgacg cgtttgcccg cgcgctgtcg gcgaaggcag gcaccaccat
     7681 caccgccgtc tacgaaccaa ccgaggccgg cggcgtgaag cagctcggcg gcgctgctgt
     7741 tggcgtcgta tccctcccgt tcttcctcgt acacgaaaaa gatctcggga tgcatgcgcg
     7801 attggtagcg gtgcaagaag ggcgcccagc gctcgagtcg tgggtgttgg tcgcgcaaaa
     7861 aggcaggatc cataaggcgg aggagctcgc cggcttcacg atcgcgtcca gcgcagcgtt
     7921 cgcgcctgga ttcgtacgcg ctgcgactgc ggggctcgga gcgctgccgg ccaacgtgaa
     7981 gctgcaggag atgaccgcgg tgctgtcatc cttgcgccgc gccgccgctg gcgaaccgat
     8041 cgcggtgctg ctcgacggac cgcaggcggg cgcactcaag tcattggcgt tcgcggacaa
     8101 gctcgacgtc gttactcgct cgccggcgtg gccggccggc atcgtggtca gcctcgacgc
     8161 gcggatgccg gcgaaggcat gggcgccgat ccagacggcg ctgaccgggc tcgctgcgga
     8221 tcgagcagcg gcgcaaacgc tggcagcgat ccagatcgcg aagttcgtcg cgctcgacga
     8281 gaaatcgctc gctagcgcac ggcaggcgta cgggaggaag ccgtgaagtc cgccgtcctc
     8341 gcaagcgttc tgttctgcgc cgcatgctcg tccgcactga aggaaccatc tccgatcgcg
     8401 acgtacgcac ctgggcacgc ggacggacgg accgcgaacg agctcgtcgc ggcagcgaat
     8461 gcggcgtggg cgcaccgcgg tgaggccggt aaggcggagg ccgcgcaagg gctgtatctc
     8521 gacgccgcgg tcgtcgaccc gcaccgcttc gacgcgatcg ccggtgccat gcgcgcgatc
     8581 tcgtatcgca tcgagaacga gcagcatgtc gcgcgcgaga agcttgcaaa gatcagcgtc
     8641 gagctcggcc agttgtgcca gcgccgtgcg ccgaagcagc ccgagtgtga ctatcggctc
     8701 gcaatcgcgc tcggccagta cgcacgcgag aacacggcga gcgccaagga tgcggtcaac
     8761 cgtatggtcg gtctgctgca tcgcgcgatc gcagcggcgc cgcgtctcga tgccggcggt
     8821 ccccaccgcg tgctcgcata cttgctgctg cgcgcgccgg gctggccgct cggcccgggc
     8881 gacgaagaag cggggctcgt acaagcgcgc gcggccgtgc agctcgcgcc gaatgcagag
     8941 aacgagctcg tactcggtga ggctctcgcc aagaacggtc atccaaacga agcgcgcgcc
     9001 gcgtacgcga aggcagccga gctcgcgacg gctgcgcacg aggcgggcga gcccgaagcc
     9061 gcgcgctggt tagcgcaagc tcgggccggc ctcgcgaaga acagcggtta gcgcgtcgac
     9121 cgcgcggcaa cgatcgtacg accggtcgtg cgcccgccgc cgatgccgag gtcgtgcagc
     9181 tccgcgtcgg tgagacgtgc ggacagcgtg gtgcgaaacc actcgaacga ctctggcttc
     9241 gcgatgggtg agtgctcgac gatttcgggt agggcaggca tcagctcggc gaattgcttt
     9301 gcgagcgccg cgttgtcgtc gacgattgcg gccgtgtccg caaggagtcg cccgacctcg
     9361 tcccgtcggc tgcgcttcgg ctcatcgagc tgcgcgatcg tgaccgcgcc cagcatcaac
     9421 gaccacgcgc agaagatccg gtactggtct ccctcgggaa tcgcctggat gtacgcgagc
     9481 gcaccctgcg catccttacg gaggctcgcg atcagatcgg tacgatttgg cacgaagaag
     9541 cgcccggcgg cttcgtcgtc gtgttggtcc ttcaggatgt tcaccttctg aaggaagaga
     9601 ccgaagtggt acgcgagtga cgtgtgcggt gctggcgcgt tccgctcgag cgtccacagt
     9661 tgcgtcagca tctcgccgac aacacctgca acgaagcagc agtaccgcgc gacgtcctcg
     9721 acatcgacga gccgcagcgg cgttggtcgc ctcgtgtagg ccgccatgcc catcgccatt
     9781 cgatccatcg cgcggaacga ggccgctcgc gccggccagg gcagggcatg tccctcggcg
     9841 agcaggtcgt acgtttcccc gagcagcgag cgttcgccgg cggtgaggtt cgtcgggaat
     9901 gccgcggcaa acgtatcgac ctcctggcgt gacggcatgg cccgaaggaa cgcgcgcagc
     9961 cgttcgaact gcttttgctg ctctgccttg tcggtgaacg gcgcatcctc gatcgtgtcg
    10021 atcacgcgaa acagcagata cgcgagcgcg acctgatctc gaaacggcgg gccgagctgt
    10081 ggaatgcaca gcgcgaagct acggctcacg acatcgagcc aatgctggta atacgaccac
    10141 ggcttcatac gacctttcct ctccggcgaa cagcagcgcg aaaacgcccg agcgcgagta
    10201 acggaaagca gtcgcggtac aggtggtacc gcaagaggaa tgcggaacgc agctcgcggc
    10261 cttgtcggat ccgcgcaccg cgttgctcgc cgacgccata acctgggaag ccgcatccgg
    10321 tgtatgcgtg ctcgtcccag ccaccatcgg cgcgctggtt atcgaggagg tagcgggctg
    10381 cgcggtgcgc aacggtcgag tccgcgcgat cggcagcgac gaaaccgagc aacgcccatg
    10441 cggtctgcga cggcgtgctg acaccacgcc cgcgcaactt cggatcgatg tacgactcgc
    10501 aaccctcgcc gaacccgcca tcgcggttct ggtgatcgac gagccagcgg atagcgcgcc
    10561 gcaccgagat gctgtccatc ggctcgccga tcgcagcaag tgccggcaac accgcgcccg
    10621 tgccatagac gtggttcacg ccccaacgtc cgaaccacga gccctcgtgc tcctgttcgg
    10681 cgtaaagata atcgagcgcc ctcacgatgg cggggtgacc gcgatcgaag ccgagcgcgg
    10741 cgagcgtctc cacgacatgc gcggtgacgt ctgcactcgg cggatcgagc atctcgccga
    10801 aatcggcgaa cggaatcatc gccgggagac gtgcggtgtt gtccttgtcg aaggcagccc
    10861 agccgccgtt ctcgctttgc attgcaagga gccatgcgac ggcgcgcttt tgggtctcgt
    10921 catcgagagc gccgacgcgg tgaagcgcga gaagcacgac ggcggtgtcg tcgatgtcgg
    10981 gatagagatc attctcgaat tcgaacgccc aaccgctcgg ctcgacgttt ggaacccata
    11041 cgcaccagtc gccggggacg cggacttcct ctttgcgcaa ccaccgtgtc gcggtctgga
    11101 tcgcgggatc cgccggcgcg acctcggact cgacgagcgc ccagaccgag agtgccgtgt
    11161 cccagacggg cgataggcat gcctgcacgc gatggcttcc gtcggctcgc cggatcatcc
    11221 atttgtcgtg catgccatcg atgcccttac gaatgaccgg atggtcagag cggtagccga
    11281 gcgtgtgcag ggccatcagc gagtacaccg tcggcggttg gatcccgccc cagctaccat
    11341 cggcctcctg gtgctcgacg atccaacgct cggcggcctt gcgcgcacgc tcgcgcagcg
    11401 gatgccatgg cagccgctgg tactgacgga gcaccgcatc gatagcgagg aacacgcgat
    11461 cgatcgggtt gcgtggtgtg ggtgcgactg ttgcggtcgc gatgcgtagc tcggcaagtc
    11521 ccgctgccgg tggaacgggt cgtgccggcc ggtcattcat caccagcagc agcgggacga
    11581 tcgtcgcgcg cgcccacgac gagaaccgat agatactcat cggggcacga tcgggaagca
    11641 gcatcagctc gagcggcatc gtgggtaagt cgtcccagct ccactctccg cagatcgcga
    11701 gccagatccg cgtgaagacg cgcgctccgg caatgccacc gcgggccatc gcgaactcgc
    11761 gcgcgcgatc gaggtgcggt ccggtgtcgc cggcgagctt gagtgcgaag tagcactcga
    11821 tcgtcgtcga cagatcgccc ggtgcaccgg cgtagagcgc ccagcttccg tccgcgcgct
    11881 ggtagctgcg gatatcctga gcaaccgcgc gccagatggc atcatctcgc tcgccgatga
    11941 cgtgcgtcat cagaatgtac tcggcgtcca tcgtcgcgtt cgattcgagc tcggcccacc
    12001 agtagccatc cgcgaattgc tcgcgccgga atcggtcgtg tgcgcgcgcg atcacctgat
    12061 cggcaccagc gcgcgcgtcg tcgatctcgg gctcgaccat ggactaactc ctctcctgcg
    12121 cggctgcagg ctgtagttgg aaccaacgca ccgctttctc gagcgtcgtg cgtactggcg
    12181 tctgtggaag tccgagctcg cggatcgcgc gagcgctgct ggcaaatcga atctcgtgcg
    12241 cggtgcgtac gccctcgagt gggatgcgcg cgtgccgcct gaacaggaac ttgtccgcat
    12301 aggcgaggcc gagtgcgagc gcgtatggca agcgcaggcg cggggctttg cgaccggaga
    12361 cctgggcgag gagctcgagg agctcgcgca tcgtcaggtt ggcgttcccg aggatgtaac
    12421 gctcgccgcg tcggccgcgc tcgagtgcga gccagtggcc ttcggcgaca tcatcgacat
    12481 cgacgacgtt catgccggtg tcgacatacg ccggcaacct gccatgcaag aagtcgcgga
    12541 tcagcgcgcc ggtcggcgtc ggcttgacgt cgccccagcc gatcggaaac gtcggattga
    12601 cgatcacggc atcgacgccg cgcactgcct cgtcacgtac cagccgctcc gcgcgccatt
    12661 tgctctcctt gtacggaccg ggcgcgatgt ggcccgcatc ctggccctcg tcggggatgg
    12721 cgccctcgcg catgccgccg atcgtggaga tgctggaggt gtagacgagt cgagcaccgg
    12781 ccgcgcgtgc cgcagcgatc acgttgcgcg taccaccgac gttggtctcg tgcagcgctg
    12841 ttcgatcggc ggcgaacgag tacaacgcgc cgacgtggaa gacggcggcg caaccggcgg
    12901 cagcggcgtg caccgagctc gcgttgcgca ggtcgccacg cacgatatcg accgctgctg
    12961 ggtctgggac gttcggtgtg gttcctgggc ggagcaacac gcgcacgtcg atgcccttgg
    13021 caagcagcgt tcgcgcgacc gcgccgccga tgaatccgct ggcaccagtg acgagtgcct
    13081 tcacgagtgc acctcgcatg cgccgagctc tccgagcacc ggcatcagct gctgcatggc
    13141 tcgtcgcaac gagcggaatg cgatcgccgt gcgagtgccg agtcgggcaa ggtcgagaac
    13201 cgcccgtggc ccgcggagcg tgtgacgcac gacggacggg acatagtgct cgcgtgcctc
    13261 gctcgcgaac cgcggcagat cgatctcgag tggatcgagc acgacgcgca gggccagcca
    13321 cggaattccc gcgcgctcgg ccgctcgcgc cgatgcccag ctctcgagat cgacggcgag
    13381 tcggcccggc cgtgcgagaa tgcgcttgtc ctcgcgcgtc gctgcgatcc gcgtcgcgca
    13441 caacagctcg ccttcagcga cctgcgtgta cgccagcgcg cggcgtgcga tgcgcagcac
    13501 ggactcgcgt gccatgacgg attcgccgct ctcgtcgcgg accgaggacg cgagtaccag
    13561 atcacccgcg ccgagcgact tctcgagcgc gcctgcgaag ccacagttga tgacgagcga
    13621 aggccgatct gcggcgacga cgtgcgccgc acctccgccg accccgacgg cgcgcgcgat
    13681 cacgcgatgc tcgccaagca tggcggatgc tgatgtgccg cgcaccttcg agcggagaac
    13741 ggacgcgatg ccacgcagct cgatcggcag cgcgaccaag atcagcgtcg ggcgttgttc
    13801 ccattctccc catgccgagg tctcgcgtgc ctcgagcgag tcacgccgcc gctcccaccg
    13861 acgttgcgct ctcgccgcac tgtcgtcgcc ggtgatcgag atgtccgcga tgtcgcgaag
    13921 cggcacggtt actcgctgcg tcgcgtcctc gtcgaacatc gcgatctcgg cgacgtcctg
    13981 ctggtagaca aatccgacga gactcgagcc gtcgtgccgg accacggtga cgtagccgcg
    14041 gtaatcgaac gcctgctgga cgagctgcat gaggcgcgtg tcggtcatgg acgccccgtc
    14101 accatggatg cgacggtcct ggcgagacct ttggtcgagg tgaacgtcga attcactgcc
    14161 gtgggctcga agccgcaatg gaccatgcag tcctggcagc gcgggttacc gctcgcgtgc
    14221 ccgtacttcg accaatcggt cttctccatc agctcgcgaa acgtgttcgc gtagccgtct
    14281 tgcagcaggt agcacggtcg ctgccagccg aagatgttgt aggtcgggtt tccccagggc
    14341 gtgcactcga agtcgagctt gcccatgagg aagcgcaaga acagcggcga ctggttgaag
    14401 cgccacctct tgtgaggtgc atcgaagatc tgtgagaaca agcggcgaga ccgctcgcgc
    14461 ttgaggaagt gttcttggtc cggcgccttc tcgtacgagt agccgggcga gatcatcatg
    14521 ccctcgacgc cgagctccat catgtcgtcg aagaacttgc ggacgcgttc gggggacgcg
    14581 ccgtcgaaca gtgtggtgtt tgtcgtcacg cggaatccgc gacgcagtgc ctcgcgaatt
    14641 gcggcgacgg ccgtgtcata gacgccctcg cgcgccaccg atcgatcgtg gtctacgcgc
    14701 ccaccatcca tgtgcacact gaagctgagg tgcttgtcgg ccttgaactc gtcgagtctc
    14761 tgctgcagca acagtgcgtt cgtgcacaag tacacgaagc gcccgcgctt gctgaggccg
    14821 cgcacgatct gcggaacgtc ggggtgaagg agcggttcac ctccggcaat gctcacgatc
    14881 ggagcaccac actcgtccga ggccgcaagg cactccgcga ccgacagctg cttcttcagc
    14941 agtgactgtg gatactgaat cttcccgcag ccggtgcacg acaggttgca gcggaacaac
    15001 ggctcgagca tcagtacaag tgggtagcgc tctcgtcctg cgagccgttg gttcactacg
    15061 tagcgcgcca ccgcatacat ctgcgcgagt gggattccca tgagtcgtcc ttgtcgtcct
    15121 tgtggaagtt ccgcgtgcag cgctgcaacg caaagcgaac gacgtgccac tgcaaacgtt
    15181 gcgataccga tggctgcggc ctgcgagatg gcgtctcggc gcgcgcgacg tgcctacgtg
    15241 gtgtgtcgct ctcgtcttgt ggacatcgcc attggcgcgg cgatcaacgc aatcgctcca
    15301 cggtctagcg gtgcacgcgt cggaatatcc attgcaggtg gctcacggcc atgtggtcgc
    15361 gtttgtcatc gctccgttat gcgtcgacgt tgctcgtcat cagcgcgtcg caccttatgt
    15421 ctgtggcgca cgcggatccg gcgccgacgc cggacgtcgc aggcgcaccg ctccccggga
    15481 acgagagtgg ccagacatgg aacgacgagg gcgagccacc ggaaggctcg ttgccgcgaa
    15541 atctcgggcg cgcagtcctg ttcatcccca agtcgctagc gtggctcctg ctacagccgg
    15601 tgcgcggcgc tgtctatgta tatgaccgct acgatctgga gaaccgctac tacgagacgt
    15661 tctacaacga gtcgcgaacg ttcggcatcg ttccggtgat cgagtacgcg accggctttt
    15721 cgatcatggt tggcggtcga ctgatcgcga aggacgtctt cggcgcgcaa gagaactaca
    15781 ccatgatggg agcgtggggc ggcgagtatc agtcgcgtgc agaagcgtgg ctcgatagcg
    15841 gcactcgctt cgatccggtg acggtgaccg tcggcggcaa cttcgatcgc ttcgcgcggc
    15901 tcgcgttcta cggcatcggc aacaactcca atagactgga catgccgccg ccgacactga
    15961 tcaatccgct cgtcaatgac accgcggtgc agacgttcta ccgctaccaa gagatgcgcg
    16021 ccgcgttgac ggcggattgg cgcctgatcg acgacttcca tctcctcgcg cacggcgcgg
    16081 tgaccggcat cgtgaccgac gagtcgacga aagatccgtc gatcgagacg gtctacaacc
    16141 cgatggacct cgtcggtttc gacgagatga caacacacgt ctacggacag ctcgaagcgc
    16201 gctgggatac ccgccgtaac gcggtcccca cgtgggaaac cacgcagtac tcgaccggct
    16261 acctcgtcag cggctacgtc ggcggcgtgc aaggtctcgg cgacgcgcac aacttcgcgc
    16321 actacggtgt cgatctacaa gcgttcgtcc acctcgcact cgggccgcgc atgttgtggt
    16381 tccggttctt gggggaaggc gtgaccggag acctaaacga gatcccattc gccgagcttc
    16441 cgtatctcgg cggcgacttc ttgcggggct acgacttcgc gcggtttcgc gatcgcgtct
    16501 cagcgctcgc agttgcccag tacacctggg atctctctcg ctttacgaac gcgtatctat
    16561 tcgtcgatgc cggtcgcgtt tatcactcga tcgaggacgc cacgctcgac gacatgaatg
    16621 tcggcttcgg cggcggcatc gagctccagt cctccaaaag cttcctgctc gcggcgacgc
    16681 tcgcgtcgtc gatcgatggc ggcgtgttcg tgtctgccgt tcttgccccc taccacagtc
    16741 aggtgccgcg atggcgatga gctctctacg ttatatggcc gtgatcgtcg tggcgacgac
    16801 gagctgtaag cagtcggccg aagttccgcg cgttcggtat gcaaatgccg ccccggtcac
    16861 ggtcgtaaac gatcgccgtg cggtgccgac gcgcccacac gtatcgcgtc agctcctcga
    16921 tctcgacttc tacgatcgat cggtcgaaga cccgctcatg aacacgttat cgctgccaca
    16981 gaagcagcgc gcgcgtggtg tgaatgcagt cgacgaagtt cccaactcga cgtggttcac
    17041 caaccggatc ggtgtgcgtg acatgtcgcc gcaggagatc gcgaacggcc cactcggaga
    17101 tgaggggccc gagccgcaca aaccatggac cgtgcacagc acgaagcccg gcggcaccga
    17161 ggtcggcttc atcatcaccg atgcgcgcgg ggtcaagtac ggcctcggct tcgacacgcc
    17221 ggagtggccc gagatggaaa ccggcaacgc ggtcgtcgtg aaccgcttgc tctgggcgtt
    17281 tggctacaac gtgcccgacg agcgcatcgc gtacatacgg cccgaagacc tcgtgcccgc
    17341 ggcgaacgct caggtgaagg actcacaagg gcgcaggctg cggcaactcg accgcaagga
    17401 gctcgaccgc cagctcgcgc tggtctccca tgcgcccgat ggtcggattc gcgtgctcgc
    17461 gtcgcgctgg ctatcaggtg agtcactagg cggtacgaac ccgacgggtg tgcggaaggg
    17521 cgatcccaat gacgtgatcc cgcacgagga ccgccgcgac ttgcgcggac agtaccccgt
    17581 gttcgcgtgg ctcgagcacc tcgacctcgt gcgcggcaac ttcctcgaca tgctgatcgc
    17641 tccgcacgat ccagcacatc gctacgtcgt gcattacctg ctcgacttcg gcaggagcct
    17701 cggcgcgatc gcggtgatcg acaagaacct ccgcgcaaat catcgctacc tggtcgactg
    17761 gagcgacccg atctggacat tcggcaccga gccgcggtca tggggcaagc atgctgcgcc
    17821 gctgctgacg ggtgtctctc cgacgtttgt cgcgaccggc ttcgatccag gcgcgtggaa
    17881 gccaaacctg ccgtaccagc cgttcgattc ggccgatcga ttcgacatgt tctggggcac
    17941 gaagattctc ggtcggttca cgcctgaaca gatccgcgcc gcggtcgagg ccgggcggtt
    18001 ctccgatcca cggaccgtcg attacctgac cgacacgctc atcaggcgtc agcaggccac
    18061 gatggcgtac tggtacgtgc gcgtcaatcc actcgacaag ttcacggtcg gccccaacgg
    18121 tctgtgcttc gaggacctcg cgatgatggg gcggctcgtg cctggcgcgc cgacccgcta
    18181 cgagctcgcg agctacgacc gtgatgcgcg accactgggc acggtcacga tcaatgcagc
    18241 gcaaggcggt gcgacgtgca cgggttcggt gaagctgtcg aacgtcaacg gtggctacac
    18301 gatcgtgaag atcacgacgc tgcggccggt cgcgaacggt tccaccatcg tgcatctcgc
    18361 acgcgcgccc aagtcggccg agtggcgcgt gatcggcgtg atgcgagtct gagctcggcc
    18421 catcaaaaca tcgaggaggc ccatgagcgt cgtcttgttc gtgatgttgg tggcgagctg
    18481 gagcttctgg atcgtgagcg tgtcggtcgc gcgccgcgtc ttcacgccgc gcgcaggttc
    18541 gcccacgtgg ttcccgcccg tgtcgatcct gaaacctgtg cgcgggctcg atttcgaagc
    18601 gcgccagaac ttcatcagcc actgcacgca gcgctacccc acgtacgaga tcctgttcgg
    18661 tgtcacgagt ggcgacgaac ccgtcgtgcc gttgatcaag gagctgcaac gcacctatgg
    18721 caccgagcgc gtgcagctcg tgatcgcacc gaacgaggcg ttgaatccga aggccgggct
    18781 actcgacgcg ctcgcgcaac gcgctcgcta tgacgtcatc gtcctcaccg acagtgacgt
    18841 tcgcttgccg cccgacggca tcgcgcgtgc gatcgcgccg ctcgcggatc cgtccgtcgg
    18901 cctggtgacg ctgctctatc gcggagatcg cgtgctcagc acagccgctg cgttcgaggc
    18961 acttggtatg gaggcggagt tcttgccgtc cgcgctcgtc gcccgcgaag tgttacgcgc
    19021 gcctttcgcg ctcggcccgg gcaacgcggt ccggcgcgat gcgctcgagc ggatcggagg
    19081 cttctcggca gtctcgaact atctcgccga tgactaccag ctcgggtttc agatcggggc
    19141 caccggaaag caggtcgagg tcggtgacgt cgtgatttcg gccgtgctcg gaccgacgtc
    19201 gctcggcgtg tggtggcagc gcgaggtgcg gtgggcgcga acgatccgca tgagccggcg
    19261 catgcactat ccggggctca tgttcacgtt cgcgacaccg ctcgcgttgc tagccacgct
    19321 cgcctacggc tggtggccgg cgctgatcgg gtcactcgcg gttcgctggt acgtcggcgc
    19381 acagatgacg cgctggatgg gtgatcgggc gctgcggcgc tggttgtggc tgttgccgct
    19441 gcgcgatctg atcagcccgg cgatctggtt tgcaggacta ctcggcaaac gcgtgagctg
    19501 gcgaggccgc acgtttcgcg tcgcaccgga cggtcagctc tacgagccgc cgcgtgatgt
    19561 gaaggcggcg cgggtgacct gatggtcgag cccaggacgc ggatccgcct gcgcacgttg
    19621 ctgggatcgg tcctgggagt cggcttgctg gtcggcgcga ccgtgttggc agcgtcgtac
    19681 ttcacggatc tgcgaacgct gctgcacgtg ctcgcgaacg gccagtggcc gtggatcgca
    19741 ggagcggtcg gcgcacaggt cgcatacctc ttcggctacg cgctgttcta ccggcttggc
    19801 ttcgcggtgg tcggcgtctc gagtcgcacg tggtcactcg tcccggtctt gttcacggga
    19861 ttgttcgtca accagctggt gccgaccggc gcgggtgccg ccgcggtctt cgtcgacgat
    19921 gcagttcgtc gtggccagag cggcgcgcgc gtgaccgtcg gtgtcgtcct cgtgctcgtc
    19981 ctcgatctgc tcgccgtcct gccgttcgtc gtgtggggaa tcgcgttcct cgtgcacagg
    20041 caactgttcg cgtcatggca gctgtttgcc gcaggcgcat tcgccatgta cgtcgtgttg
    20101 ctcgtcgtgc tgctcgcgct gtcgcacgca aagcaggcga gcgtgtgtcg agcgctcacg
    20161 atctgctatc gcgtgatcca tcgcgtgctc gcctggatcc gcgtgcgcgg cccgtcggtg
    20221 gactggcccg cgcggaccgc tgctgggttc gctgatgccg cagcagcgat cgcggcgaac
    20281 cgtggccagc tagcgctcgc cggcgtgtgg ggcatcgcga ttcatctgct gaacgcgttt
    20341 ggcctgtggt tgttcgtgcg cggctttggc gccgacgtcc cgctcggcgg gttgatcgcg
    20401 gcatacgcgc tcggcgtcgt actggtcgtg attgccgtga tcccgcagtt cgcgccgatc
    20461 tctcaggcat tcgtcaccgc gacgttcatc ggcgtcggta tggccaccgg tcccgcagtc
    20521 gcatcgacgc tcgcgtttcg cggcctcgct ctgggattgc cgctcgtggt cggtctgccg
    20581 ttcgcgtatc ggttcggtcg gtttcgcgtc gacgtactac gaggggcaac atgatcgccg
    20641 tcgacttcgc tcggttcgcg atcgggacgg ttgtgctgta cgccgcgacg accgtcgtct
    20701 tcgacgtcct gcattacgcg ttgcatcgct gtgccgcttc gcgtttccgc gtgttgcgga
    20761 gggtggcagc gccgcatgtc gtgcatcacg agttcctcgg cacgtcgttg cggttcgacg
    20821 agcggctggt gtggccgaac atctggtggc accgcgtgcc ggaatggacg gtgcaagtcg
    20881 cggcgaccgc gtgcgctgcg ccgttcgtgc cgctgtcgtg ggtcttgtgc gcagcagcca
    20941 cgcatacggt gttgttcgtg atcacggtgg tcggtcgcgg caaggacggc aaccacgtga
    21001 gcttgacgac ggtgccgtcg ccacggtcgt ggttcgtcgg ccctgcgtac cactcgctgc
    21061 atcacgccta tcccgagagc tacatgagct cgttcgtgaa gctgttcgat tgggtgctcg
    21121 gaaccggttg tcacctgcgc ggaaagcgaa tcgtgatgac cggcgcttcc ggtgcgttcg
    21181 gagagccgct ccggcaactt ctcgaacaag aaggtgcggc cgtcactgcg ttgcgattcg
    21241 gccacgactg gacgtacgac gactacgaag gcgtcgatcg ggcgctcgcc accgcggaca
    21301 tcctggttct cgcgcacggc gcgaaggggc cacaggcgat gcaggccaac tgcgattcgt
    21361 tcgtcgcgtt catcgaacga tttctctcgc ggcatccaaa cgcgctgatt ccgatcgagg
    21421 tgtgggcggt cggatccgag atcgaagcgc atcccgcctg gggaaacccg gagcttcagg
    21481 tctatctcga gtccaagcga gcctacgcgc ggcatgcgtg gcgatacttt cgcgatcgcc
    21541 ggtttacgta ccgacacatc gtgcccgcgg cgtttcgttc ccgcatgggg cccggattga
    21601 tgtcgggccg aaccgcggca cggaccgcca tgttcttcat ccggcgcggc tttcgctacg
    21661 tcccggtgac ctacaccggc atcgcgttcg tgaactattg gaagttcgtt ttcagccgac
    21721 gcgatacgag cccgacgttg tgacatgttc ggggcatgaa gctgggcctc gtactcgcgc
    21781 tcgcgcttgc gagctgcggt ggcaaatcac ccccggccgc gcactcgaac gtcgccccga
    21841 gcccgacggc accaggcgtg ctgcgcgccg tcgctgactt cgacgcaatc ggtgatccgg
    21901 cggcgcgctc gcgagcattg ttcgccgagg cggcgcgtgt gctcacgcac ccccgttgcg
    21961 tcaactgcca tcccagcggc gacagcccac accaacgcat ggcgatggag atgcacgatc
    22021 cgccggtcgt gcgcggtccc gcgaacaacg gcgtgccagg catggagtgc acgacctgtc
    22081 atcaggatcg gaaccaggcg ctgacgcgcg tcccgggcgc accgaagtgg cacctcgcgc
    22141 cgatcgagat ggcgtgggtc ggcaagtcgc ccgcgtacat ctgcaaccag atcaaggatc
    22201 ccaagcgtaa cggcggcaag acgctcgcgc agatcgtcga tcacagcgcg cacgacgcgc
    22261 tcgtcgcgtg gggctggtcg ccgggcgcgg atcgcgagcc agtgcctgga acgcaggaac
    22321 agtttggtgc gctcgtcgag gcgtgggtca agaccggcgc cgaatgcccg gaggagacgc
    22381 gatgaagatc aagatcaacg gcgtcgagca gacgctcgac atcgatcctg acatgccact
    22441 gttgtgggcg ctgcgcgacg tgctcgggct caccggcacc aagtacggct gtggcgaggc
    22501 gttgtgcggc gcgtgcaccg tgcacctcga cggccgtgcg gtgcgcgcgt gcgtgacgcc
    22561 ggtccgccga gccgaggggc gctccgtcac gacgatcgag ggcctgtcgt ccgatggcag
    22621 tcatccggtg cagcaagcgt ggctcgacct gcgcgtcccg cagtgcggct actgccaggt
    22681 cggtcagatc atgacggcag ccgcgctgct cgaagcgaag ccaaagccga ccgacgccga
    22741 gatcgatcag tcgatggccg gcaacatctg tcgctgcggc acgtacacgc gcatccgctc
    22801 tgcgatcaag aaagctgcgg gcatgcccga cgacggaggt gacaagtgac gcctccgatc
    22861 aagctcacgc ggcgctcgtt catcgctggg ctcggcgctg gcctcgcggt cggcgtcatg
    22921 ccgcgcatcg cgcacgccaa agagccgacg aaagagcagg ggcgcggtct cgatcctgac
    22981 gtgctcgttc acatcgcgat cgacggcacg acgacgatcg tctgccatcg ctccgagatg
    23041 gggcagggca ttcgcagctc gctgcccgtg ctgatcggcg acgagctcgg tgccgatccg
    23101 gcgcgcatcg tcgtgcgcca ggccgagggc gacaagaagt atcccgacca aaacaccgac
    23161 ggctcgacga gcattcgcaa gcagtacgac gtgctccgcc aggcgggcgc ggccgctcgc
    23221 atgatgctga tcgccgccgc tgcgaaacgg tggaaggtaa agccggagac ctgcaccacc
    23281 gcgaaccaca ccgtcatcca cacgccgacg aagcgcgcac tcgcgtttgc cgaggtcgtc
    23341 gatgccgctg cgaagctgcc ggtgccgaag cgcgagagca tcgtgctgcg cccgatcggc
    23401 gagctgaaga acgtcgcggc gccaacgctg ccactgctcg atggccgcgg ctacgtcacc
    23461 ggcacggccg agtacggcgc cgacgtgaag cttcccggca tggtcgtcgc ggtgatcgcg
    23521 cggccgccgg tcgtcggcgg caaggtcgcc aagtacgacg cgacacgcgc gctcgccgtt
    23581 ccgggcgtca agaaggtgat cgagatgccg gcggcgaaac cgccgtggga cttccagccg
    23641 tggggtggca tcgccgtcgt tgccgacaac acctgggccg cgctcaaggg acgcgccgcg
    23701 ctcgagatca cgtgggatca cggtccggag gcgagcttca attcgtcggc gtatcacgac
    23761 gagctgctcg cgtcggtgcg cggacccggc accaaggctc gcgacgttgg cgatatcgac
    23821 gccgcgttcg caaaggcggc gaagaccgtc gaggccgagt acttcgtgcc tcatctctcg
    23881 cacctgcaga tggagccgct cgtcgcggtc gcgcggatca cgaacggcaa cgtagaagtc
    23941 tggtcgccga cgcaggggcc acaagacaac cgcgaggaca tcgcaaagat cctcggcatt
    24001 cccgaggaca aggtgagggt gaacgtcacg ctgctcggcg gtgcgttcgg tcgcaaggcg
    24061 aagtccgact tcccgatcga ggcggtgtac atcgcgaagc aactcggcga tggcgtgccc
    24121 gtgcgtgtcc agtggacgcg cgaggacgac gtcaagcacg gctactacaa cgcggtgaac
    24181 gcgcagcgtc tgcgcgcggc gctcgacgac aaaggcaccg tcaccgcctg gcatcacaag
    24241 accgcgttca cgccgatcaa aggcacgttc gacgacaagg tcgacacgcc gtcgatcgac
    24301 gatctccagc aaggcgtcac cgatctcgcg ctcgccgcgc cgaacgtgcg cgtcgaggca
    24361 tgcaaggcgc cgctgcacac gcgcgtcggt tggttccgct ccgtctacaa catcttccac
    24421 gcgtttgcga tcggctcgtt catcgacgag atcgcgcacg cgcgcggtgc cgatccgcgc
    24481 gatacctggc tcgacgtgat cggtcctgcg cgcaagctat cgctcgctga tctcggcgtc
    24541 gcgcggctcc gcaattacgg cgagacgctc gacaagcatc cggtcgacgc gggccgcctg
    24601 cgtaacgtga tcacgcgcgt caccgacatg gcg
//
LOCUS       DASUFX010000795         4699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1796239, whole genome shotgun sequence.
ACCESSION   DASUFX010000795 DASUFX010000000
VERSION     DASUFX010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796239"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /gene="pyk"
                     /locus_tag="VFV99_32150"
     CDS             <1..1488
                     /gene="pyk"
                     /locus_tag="VFV99_32150"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5064088.1"
                     /translation="RSPVITGACGFQQAQWVLYWPRKMPRAKIVCTLGPASATPDRVG
                     ELIDAGMNVARLNFSHGSHEDHAKMLQVVRSEADRRGKAVAVLLDLQGPKIRVGKFAN
                     GQIELKPGAALTITTDTSVIGDEKRVSTTYAQLPLDVKPGDQLLLDDGYLSLAVTDIE
                     PKEVRTVVVTGGILKNNKGINLPGVEVSAPALSEKDRTDIGFALRYGVDYVALSFVRR
                     PEDVLEAKRLLTVDQVSIPVIAKIEKPQALERLIEIVEVTDGIMVARGDLGVELGPEK
                     VPLWQKRIIEETNKRGKIVITATQMLESMITQARPTRAEASDVANAVLDGTDALMLSG
                     ETASGAHPVEAVRTMARIIDEIEKSAYYRANIEIPIIEQVSSANAIAHAAVSAAKAMK
                     AKTLVVVSDSGGAARLVSEYRPEASLVCLTTDEVTFRRLALFWGVTPVLIGPSATTEE
                     LLDRVEGTLLERNLALPGENILITMAVPVGSGMQTNVLKIHQIQH"
     gene            1625..3421
                     /locus_tag="VFV99_32155"
     CDS             1625..3421
                     /locus_tag="VFV99_32155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064089.1"
                     /translation="MAQTVKPADAGVSIGQVFQGKYRVDSIVGHGGMGVVAECTHLAL
                     NERVAIKMLRQDVLLDQDAVSRFIREAQAAVKLKSEYVARVSDVGTFENGVPYMVMEF
                     LEGHDLGELLKQRGVLPVQWAVELMLQTAEALAEAHSLNIVHRDVKPTNLFVTWRPDG
                     SALIKVLDFGISKSPLGTDMQLTQTQSLLGTPAYMSPEQMRSARLVDTRTDIWSLGTV
                     FYEILEGRRPFEAESFSEMCVKVAVDPPAPMINTPPGLQQVILRCLAKSPEQRYANMA
                     ELGRDLVPYSPDPHGAQMLVDRMARMLRRSGIATQADWDGSTGTGVGSNPGRVPGSAR
                     ELGSGPVPRAQTPLPQQWGIGSDPAAAPWHGGTPRGDGSQPFARPINEASQPFARPLA
                     DDPSAGFAGPTVSTKKSRAPLIILMLALLAAAGVGIGFYATSGDTPVAQKQQTPDENK
                     VEATQTMPVTPAETKTGETQPAVIDTKAADTTGTNAVDTKATDTNATDTNATDTKATD
                     TKAVEAKASDAKVETKAEIKKTGTTKTGTAKTGTAKTGTAKTGTAKTDVKKTGTNAAG
                     ETAGETKEIKPEKKEPEKPCNPFASMHGCPAK"
     gene            3453..4370
                     /locus_tag="VFV99_32160"
     CDS             3453..4370
                     /locus_tag="VFV99_32160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5064090.1"
                     /translation="MRSVVVLALVVGSYGVALAEDDPDKADKVFAEAQQLKDAGNTAE
                     ACKKFDEALSYNRNAVGTLLNVALCNEEAGKYATAVKYYTQARDLAREHNLAEHRKAA
                     EDKLALTSPLVSHLAIAFAEKANEMKLVIDDEVVPLDKTDDIVIDPGSHHVVVTAPDR
                     VPWETTVVVDKSSAKALAVPKLGYPVTVKKGRRTAAMIFGAAGGAMMLSGVALGLVAR
                     SKYNGQVGPGKACTDESPPRCDSEGYRITNDARTYGNFGTYIGIAGIAVTGVGAYLWF
                     FGPKTSERNVAVVPTLAPESAGITAVGRF"
     gene            4385..>4699
                     /locus_tag="VFV99_32165"
     CDS             4385..>4699
                     /locus_tag="VFV99_32165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064091.1"
                     /translation="MRRLGVSLAVVSWVLVGTAAADDGKQKRADELFEQGRKALAEAG
                     DDKTKIATACEKFDEAIKLDPEAPGTMLNLGLCNEKLDKYKTALYWFRKAQARASETK
                     LLD"
BASE COUNT          944 a         1520 c         1535 g          700 t
ORIGIN      
        1 cggtcgccgg taataaccgg agcgtgcggt tttcagcaag cccaatgggt gctctactgg
       61 ccgcgcaaaa tgccccgggc gaagatcgtt tgcaccctcg gccctgcgtc ggccacgcct
      121 gaccgcgttg gcgagctgat cgatgcgggc atgaatgtcg cgcgtctgaa tttctcgcac
      181 ggcagtcacg aagatcacgc aaagatgttg caggtcgtgc gcagtgaggc ggaccgtcgc
      241 ggcaaagcgg ttgccgtgct gctcgatttg cagggtccga agatccgagt tggcaagttc
      301 gcaaacggtc agatcgagct caagcccggc gccgcgctca cgatcaccac cgataccagc
      361 gtgatcggcg acgagaaacg cgtgtcgact acgtacgcgc agcttccgct cgacgtgaaa
      421 ccgggcgatc agctgctgct cgacgatggc tatctgtcac tcgctgtcac cgacatcgaa
      481 ccgaaggaag tgcgcactgt cgtcgtcact ggcggcattc tcaagaacaa caagggcatc
      541 aacttgcccg gcgtcgaagt gtcggcgccc gcgctgtcgg agaaggaccg gaccgatatc
      601 ggcttcgcac tgcgctacgg cgtcgactac gtagccctat cgttcgtgcg ccgtcccgaa
      661 gacgtgctgg aggcgaagcg actgctcacg gtcgatcagg tgtcgatccc ggtgatcgcg
      721 aagatcgaga aaccgcaggc gctcgagcga ctgatcgaga tcgtcgaggt caccgacggc
      781 atcatggtcg cgcgcggcga tctcggcgtc gagctcggtc ccgagaaggt gccgctctgg
      841 cagaagcgga tcatcgaaga gacgaacaag cgcggcaaga tcgtcatcac cgcgacgcag
      901 atgctcgagt cgatgatcac gcaagcgcgg ccgactcgcg cggaagcaag tgacgtcgcg
      961 aacgcggtgc tcgatggcac cgacgcgctc atgctctccg gcgagactgc atcgggcgcg
     1021 catccggtcg aagccgtgcg cacgatggcg cgcatcatcg acgagatcga aaagagcgcg
     1081 tactaccgcg cgaacatcga gatcccgatt atcgagcagg tcagctcggc gaatgcgatc
     1141 gcgcacgccg cagtcagcgc cgcgaaagcg atgaaggcca agacgctcgt cgtcgtctcc
     1201 gattccggtg gcgcggcacg gctcgtcagc gagtaccggc cggaggcgtc gctcgtgtgc
     1261 ctcaccacgg acgaagtcac gttccgccgg ctcgcgctgt tctggggcgt gacaccggtg
     1321 ctgatcgggc cgtccgcaac gaccgaagag ctactcgacc gagtggaagg caccttactc
     1381 gaacgcaacc tcgcgctgcc gggcgagaac atcctgatca cgatggccgt gcccgtggga
     1441 tcgggcatgc agacgaacgt gctcaagatt catcaaattc agcactgacc gacggctcac
     1501 tcccgcgcgt cgatcgtcgt ctcgggtgtc gatctcggta gacagcctga tgggctcggt
     1561 acgcaaaaac ctacacgagt caaaggcttg aggcagccgc attcgcgggc tacgatttta
     1621 gggcgtggcg cagactgtca aaccagccga cgctggcgtc agcattggcc aggtgttcca
     1681 gggtaagtac cgtgtggatt cgatcgtcgg ccacggcggt atgggcgtgg tcgccgagtg
     1741 cacgcacctg gcgctgaacg aacgcgtcgc gatcaagatg ctgcgccagg atgtcctgct
     1801 cgatcaggac gccgtctcgc ggttcatccg cgaagcgcaa gctgcggtga agctcaagag
     1861 cgagtacgtc gctcgcgtgt cggacgtcgg cacgttcgaa aacggcgtgc cgtacatggt
     1921 gatggagttc ctcgagggcc atgacctcgg cgagctcttg aagcagcgcg gcgtgttgcc
     1981 agtgcagtgg gccgtcgagc tcatgctgca gaccgcggag gcactcgcgg aagcgcactc
     2041 gctcaacatc gtccaccgcg acgtcaaacc aacgaacctg ttcgtgacgt ggcggcccga
     2101 cggtagcgcg ctgatcaagg tgctcgactt cggcatctcg aagtcgccgc tcggtaccga
     2161 tatgcagctg acgcaaacgc agtcgctgct cggcacgccc gcgtacatgt ctcccgagca
     2221 aatgcgctcg gcgcgcctcg tcgacacgcg cactgacatc tggtcgctcg gcacggtgtt
     2281 ctacgagatc ctcgagggac gccggccgtt cgaagctgag agcttcagcg agatgtgcgt
     2341 caaggttgcc gtcgatccgc ccgcgccgat gatcaacacg ccgcccggcc ttcaacaggt
     2401 catcttgcgc tgcctcgcga agtcgccgga gcaacgctac gcgaacatgg ccgagctcgg
     2461 ccgcgatctc gtgccgtact cgcccgaccc gcacggcgcg cagatgctcg tcgatcgcat
     2521 ggcgcgcatg ctgcgccgct cgggcatcgc gacgcaggcc gattgggacg gcagcaccgg
     2581 caccggcgtt ggctcgaacc ccggacgcgt tcccggctcg gcgcgcgagc tcggctcggg
     2641 tccggtcccg cgcgcacaga cgccgcttcc gcagcagtgg ggcatcggtt cggatccggc
     2701 ggcggccccg tggcatggcg gtacgccgcg cggcgatggc tcgcaaccgt tcgcgcgacc
     2761 gatcaacgaa gcgtcgcagc cgttcgcccg gccgctcgcc gacgacccga gtgccggctt
     2821 cgcgggcccg acggtctcga cgaagaagag ccgcgcccca ctgatcatcc tcatgctcgc
     2881 gctgctcgcg gccgcaggcg tcggcatcgg cttctacgcg acgagcggcg atacgccggt
     2941 cgctcagaag caacagacgc ccgacgagaa caaggtcgag gcgacgcaaa cgatgccggt
     3001 cacaccggcg gaaacgaaga ccggcgagac gcagccggca gtgatcgaca cgaaggcggc
     3061 cgacacgacc ggcacgaacg ccgtcgacac gaaggcgacc gacacgaacg cgaccgacac
     3121 gaacgcgacc gacacgaagg cgactgacac gaaggcggtc gaggccaagg cgagcgacgc
     3181 gaaggtcgag acgaaggccg agatcaagaa gaccggtacg accaagaccg gcactgccaa
     3241 gaccggcact gccaagaccg gtaccgccaa gaccggcacc gcgaagaccg acgtcaagaa
     3301 gaccggcacc aacgcggcgg gcgagacagc gggcgagaca aaagaaatca agcccgagaa
     3361 gaaagagccc gagaagccgt gtaacccgtt cgcgtcgatg cacggctgcc ccgcaaagtg
     3421 atctcgccgc gcggttaggt tacgatccgc ggatgcgctc cgtggtggtg ctcgcgctcg
     3481 tagtgggtag ctatggcgtc gcccttgccg aggacgaccc tgacaaggcc gacaaggtat
     3541 tcgccgaagc gcagcagctc aaggacgccg gcaacacggc cgaggcgtgc aagaagttcg
     3601 acgaagcgct ctcgtacaac cgcaacgcgg tcggcacgct gctcaacgtc gcgctgtgca
     3661 acgaagaggc cggcaagtac gccaccgcgg tgaagtacta cacgcaggca cgcgatctcg
     3721 ctcgcgagca caacctcgcc gagcaccgca aagccgccga ggacaagctc gccctcacct
     3781 cgccgctcgt ctcgcatctc gcgatcgcgt ttgccgagaa ggcgaacgag atgaagctcg
     3841 tgatcgacga cgaggtcgtg ccgctcgaca agaccgacga catcgtcatc gaccctggca
     3901 gccatcacgt cgtcgtcacc gcgcccgacc gcgtgccctg ggagaccacg gtcgtcgtcg
     3961 acaagagcag cgcgaaggcg ctcgcggttc ccaagctcgg ctatccggtt acggtcaaga
     4021 aaggccgccg caccgctgcg atgatcttcg gcgcggccgg cggcgcgatg atgctgtcgg
     4081 gcgtcgcgct cggcctcgtc gcgcgtagca agtacaacgg ccaggtcggg cccggcaaag
     4141 catgtaccga cgagagcccg ccacgctgtg acagcgaggg ctatcgcatc acgaacgatg
     4201 cgcgcacgta tggcaacttc gggacgtaca tcggcatcgc gggcatcgcg gtcaccggtg
     4261 tcggtgcgta tctgtggttc ttcggtccga agacgagcga acgcaatgta gcggtggtgc
     4321 cgacgctggc gccggaatct gccggtatca ccgccgtcgg tcggttctag gttaagatcg
     4381 gagtatgcgt cgactagggg tctcgctcgc agtcgtctct tgggtattgg tcggcaccgc
     4441 cgccgccgat gacggcaagc agaagcgagc cgacgagctg ttcgaacagg gccgcaaggc
     4501 gctcgccgaa gcaggagacg acaagacaaa gatcgcgacg gcgtgcgaga agttcgacga
     4561 agcgatcaag ctcgatcccg aagcgccggg cacgatgctg aacctcggcc tctgcaacga
     4621 gaagctcgac aagtacaaga ccgcgctcta ctggttccgc aaggcgcaag cacgcgcctc
     4681 agagacgaag ctcctcgac
//
LOCUS       DASUFX010000796         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_268699,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000796 DASUFX010000000
VERSION     DASUFX010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268699"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /gene="murC"
                     /locus_tag="VFV99_32170"
     CDS             <1..1546
                     /gene="murC"
                     /locus_tag="VFV99_32170"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5064092.1"
                     /translation="SSRHRLVRRPALAMWYQAQACSRRRAVIQQGLGMFRKQDTKIHF
                     VGIGGMGMCGIAEVLANMGYRVSGSDMNDTEITRHLASIGCAVKQGHRADNLGEADVV
                     VVSSAIKGYNPEVEAARARSIPIIPRAEMLNELMRMKYGIAVAGAHGKTTSTTMMHTV
                     LMAAGLDPTAVIGGRVNSLGLANARWGASDYLVAEADESDGSFLTLTPTVAIVTNVDA
                     EHLDHYGTFDNVKKAFTDFCNRVPFYGMSALCADNAGVQSILPNLTKRFTTFGISNQA
                     TYRARNIRHEGLVTKFVAWRRADELGEVSLPMPGAHNVLNALGVLAVADFLAIPFETF
                     AKAIGHFEGVARRFTVRGEVGGITIVDDFGHHPAEVRATLSGARANFPNRRIVAAFQP
                     HRFTRTRDHLADFAKAFHDADAIAICDIFAAGEKPIDGVTSDALVKLCRDAGHKGVAY
                     VAKREELARWLDEQAKDGDLVITLGAGNIQLTCNEVIELFEKRLGPATKKNLVRVAES
                     SATVAR"
     gene            1543..1842
                     /locus_tag="VFV99_32175"
     CDS             1543..1842
                     /locus_tag="VFV99_32175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064093.1"
                     /translation="MKRLLVLALCLGCKEPPPKMTMAPADVINAITDYADRGCVCETD
                     KECFRAVRDEWDAAKRDLVKNARQLTGDDAAAYAEARKRFGLCGDAAGLTVFDKW"
     gene            1864..2892
                     /locus_tag="VFV99_32180"
     CDS             1864..2892
                     /locus_tag="VFV99_32180"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5064094.1"
                     /translation="MLTIERDVPLAPLTTLGLGGRARSFVRVTTVADLQHALLSDPRI
                     LILGGGSNLVVGDAGWDGLVIQIALPGVDIAHERDHASVTVGAGVVWDDFVAQMVAAE
                     LAGVECLSGIPGLVGATPMQNVGAYGQEVADTITRVRAIDRTTGDVVDIAPDACQFAY
                     RTSMFKGNARYVIVEVAFRLAKNPHSVPLRYPELTRALGVADAGRAPLADVRRRVIEL
                     RRGKGMVVDATDPESRSAGSFFTNPIVDAATFATFAARLSPDITPPSWPAPNGTTKLS
                     AAWLIERAGFAKGYTVGHVGISKKHALALVNRGDATAAELLALAREIQAGVRDKLGIE
                     LVPEPVIV"
     gene            complement(2900..3334)
                     /locus_tag="VFV99_32185"
     CDS             complement(2900..3334)
                     /locus_tag="VFV99_32185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064095.1"
                     /translation="MSLAMIGNGASYEFRWRTWALLRDVVVAHLDEESLPGFCALGDA
                     MVDGALRVRADLLLADIDRIRAWLVGRPFDDLVLGPRTSALLHFTAQPTQRRPLTENE
                     IERIRPVAGSTDLAEYFATMLDSMARVCARPAADGTVEIVDG"
     gene            3415..>4198
                     /locus_tag="VFV99_32190"
     CDS             3415..>4198
                     /locus_tag="VFV99_32190"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5064096.1"
                     /translation="MAHPFAGKRVGVVMGGLSAEREVSLNTGAGVLAALQEKSWDAVG
                     IDWKPGTSLAQLLAASGVHAVWNALHGTYGEDGAVQGLCACLQIACTGSGILASALAM
                     DKVMSKRIFESNGVPTPRWRLLPHDGPADGSDAPAALADFPLPFVIKPANEGSSVGVT
                     IVEDRAQIAAAAAAARQFHGAVIVEDYIAGTEVFVGILDGRVLGSVEVRPATKFYDYE
                     AKYKRNDTTYLIPPQLPADVIERAQQYALAAYNALGCSGHSRP"
BASE COUNT          746 a         1420 c         1381 g          651 t
ORIGIN      
        1 cagcagccgt catcgactgg tgcgccgccc agcactagcg atgtggtatc aagcgcaagc
       61 atgctcgcgc cggcgggcgg tgatccagca aggcctcgga atgtttcgca aacaagacac
      121 caagatccac ttcgtcggca tcggcggcat gggcatgtgc ggcatcgccg aggtgctcgc
      181 caacatgggc taccgcgtgt cgggctcgga catgaacgac accgagatca cgcgccacct
      241 cgcgtcgatc ggctgcgcgg tcaaacaagg ccatcgcgcc gataacctcg gtgaagccga
      301 cgtcgtcgtc gtgtcgagcg cgatcaaagg ttacaaccca gaagtcgaag ccgcgcgtgc
      361 gcgctcgatc ccgatcatcc cgcgcgccga gatgctcaac gagctgatgc ggatgaagta
      421 cggcatcgcg gtcgcaggcg cgcatggcaa gacgacgtcg acaacgatga tgcatacggt
      481 gctgatggcg gccggtctcg atccgaccgc ggtgatcggc ggccgcgtca actcgctcgg
      541 cctcgcgaac gcgcgctggg gcgcgagtga ttatctcgtt gccgaagccg acgagagcga
      601 cggatcattc ctcaccctga cgccgaccgt cgcgatcgtg acgaacgtcg acgccgagca
      661 cctcgatcac tacggcacgt tcgacaacgt gaagaaggcg ttcaccgact tctgcaaccg
      721 cgtcccgttc tacgggatga gcgcgctgtg cgccgacaac gcgggcgtgc agtcgatcct
      781 gccgaacctc acgaagcggt ttacgacgtt cggcatctcg aaccaggcca cgtatcgcgc
      841 acgcaacatc cgccacgaag gtctcgtcac gaagttcgtc gcgtggcgcc gcgccgacga
      901 gctcggcgaa gtctcgctgc cgatgccggg tgcgcacaac gtgctcaacg cactcggtgt
      961 gctcgcagtc gcggacttcc tcgcgatccc gttcgagacg ttcgcgaagg cgatcggtca
     1021 cttcgaaggc gtcgcccggc gcttcacggt gcgcggcgaa gtcggcggca tcaccattgt
     1081 cgacgacttc ggccatcacc cggccgaggt tcgcgccacg ctgtcgggag cgcgcgcgaa
     1141 tttcccgaac cggcgcatcg tcgctgcgtt ccagccgcac cgcttcacgc gaacgcgcga
     1201 tcacctcgcc gacttcgcga aggcattcca cgatgccgat gcgatcgcga tctgcgacat
     1261 cttcgccgcc ggcgagaagc cgatcgacgg cgtgaccagc gacgcgctgg tcaagctgtg
     1321 ccgcgacgcc ggccacaaag gcgtggcgta cgtcgcgaag cgcgaggagc tcgcgcgctg
     1381 gctcgacgag caagcgaaag acggcgacct cgtgatcacg ctcggcgcgg gcaacatcca
     1441 gctcacgtgc aacgaggtca tcgagctgtt cgagaaacgc ctcgggccgg cgacgaagaa
     1501 gaacctcgtg cgagtcgcgg aatcgagcgc gacggtcgcg cgatgaagcg cctgctcgtc
     1561 ctcgcgctgt gcctgggctg caaggagccg ccgccgaaga tgacgatggc gcccgcggac
     1621 gtgatcaacg cgatcacgga ttacgcagat cgcggctgcg tgtgcgagac cgacaaggaa
     1681 tgctttcgcg cggtgcgcga cgaatgggac gccgcgaaac gcgacctcgt caagaacgcg
     1741 cgccagctca ccggcgacga tgccgccgca tacgccgaag cgcgcaaacg gtttggccta
     1801 tgcggcgacg ccgccggcct caccgtgttc gacaagtggt aaccgcgtga ccgcgagcgc
     1861 gacatgctaa cgatcgagcg cgacgttcca ctcgcaccgc tcaccacgct cggcctcggc
     1921 ggtcgtgcac gcagcttcgt gcgcgtcacg acagtcgcgg atctccagca tgcgctgctc
     1981 agcgatccgc gcatcctgat cctcggcggt ggttcgaatc tcgtcgtcgg cgatgcgggc
     2041 tgggatggcc tcgtcatcca gatcgcgctg cccggcgtcg acatcgcgca cgaacgcgat
     2101 cacgcgagcg tcaccgtcgg tgcgggcgtc gtctgggacg atttcgtcgc gcaaatggtt
     2161 gcggccgagc tcgccggcgt cgaatgcctg tccggcattc ccggtctcgt cggcgcgacg
     2221 ccgatgcaga atgtcggcgc gtacgggcag gaggtggccg acacgatcac gcgcgtgcgc
     2281 gcgatcgatc gtacgaccgg cgacgtcgtc gacatcgcac ccgacgcatg ccagttcgcc
     2341 taccgcacga gcatgttcaa gggcaacgcg cgctacgtga tcgtcgaggt cgcgttccgg
     2401 ctcgcgaaga acccgcacag tgtgccgctg cgctacccgg agctgacgcg tgcgctcggc
     2461 gtggcagacg caggacgcgc gccgctcgcg gacgtgcgcc gccgcgtgat cgagctgcgg
     2521 cgcggcaagg gcatggtcgt cgatgcgacg gatccggaat cgcgcagcgc aggctcgttc
     2581 ttcacgaatc cgatcgtcga cgccgcgacg ttcgcgacgt tcgcagcccg cctgtcgccc
     2641 gacatcacac cgccgagctg gcccgcaccg aatggcacca cgaagctgtc cgccgcgtgg
     2701 ttgatcgagc gagccgggtt tgcgaagggc tacaccgtcg gtcacgtcgg catctcgaag
     2761 aagcacgcgc tcgcgctcgt gaaccgcggt gatgcgacgg ctgcggagct actcgcgctc
     2821 gctcgcgaga tccaggccgg cgtgcgcgac aagctcggca tcgagctcgt gcccgagccc
     2881 gtgatcgtgt aagcgaacgc tagccgtcga cgatttcgac cgtaccgtcg gccgcgggac
     2941 gcgcgcagac gcgagccatc gaatcgagca tcgtcgcgaa gtactcggct agatccgtcg
     3001 agcctgcgac cggacggatc ctctcgatct cgttctcggt caacggacga cgctgcgtcg
     3061 gttgcgctgt gaagtgcaag agggcagacg tgcgcggccc gagcacgagg tcgtcgaacg
     3121 ggcgcccgac aagccacgcg cgaatgcgat cgatgtcagc caggagcaag tccgcacgta
     3181 cgcgcagcgc accgtcgacc atcgcgtccc ctagcgcgca gaacccgggc aggctttcct
     3241 cgtcgagatg tgccaccacc acatcacgaa gcagcgccca ggttcgccag cggaattcgt
     3301 aggaggcccc attcccgatc atcgccaagc tcatcgctcg acgattctac gcagcacgcg
     3361 cggtgttgtg tcgcggaata gcgtgcgacc agccggcgtt gatacaacct ggtcatggca
     3421 catccgtttg cgggcaagcg tgtcggcgtt gtcatgggcg gcctcagtgc agagcgcgag
     3481 gtctcgctca acaccggcgc cggcgtgctt gccgcgctgc aggagaagag ctgggacgcg
     3541 gtcgggatcg actggaagcc cggcacgtcg ctcgcgcaat tgctcgcagc atccggtgtc
     3601 cacgccgtgt ggaacgcgct gcacggcacg tacggcgagg atggcgccgt ccagggtctg
     3661 tgcgcgtgcc tgcagatcgc atgcaccggc agcggcatcc tcgcgagcgc gctcgcgatg
     3721 gacaaggtga tgagcaagcg catcttcgaa tcgaacggcg tgccgacgcc gcgctggcgc
     3781 ctcttgccac acgacggtcc cgccgatggc agcgacgctc cggccgcgct cgccgacttt
     3841 ccgttgccat ttgtgatcaa gccggcgaac gagggcagct cggtcggcgt caccatcgtc
     3901 gaagatcgcg cccagatcgc agcggctgcc gctgcggccc gccagtttca cggtgcggtg
     3961 atcgtcgagg actacatcgc aggcaccgag gtattcgtcg gcattctcga tggtcgcgtg
     4021 ctcggctccg tcgaggtccg gccggcgacg aagttctacg actacgaggc gaagtacaaa
     4081 cgcaacgaca cgacgtacct gatcccaccg caactgcccg ccgacgtgat cgagcgcgcg
     4141 cagcagtacg cgctcgccgc gtacaacgca ctcggctgca gcggtcactc gcgtccgg
//
LOCUS       DASUFX010000797        12704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1516645, whole genome shotgun sequence.
ACCESSION   DASUFX010000797 DASUFX010000000
VERSION     DASUFX010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516645"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3536
                     /locus_tag="VFV99_32195"
     CDS             <1..3536
                     /locus_tag="VFV99_32195"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX5064097.1"
                     /translation="RKLAFDGAAWRYDGDIPANVRAQLDAELAVIEELDYPGYFLTMY
                     EIVSYCRRRDIMCQGRGSAANSAVCFCLGITAVDPIRMGLLFERFLSRERAEPPDIDL
                     DIEHERREEVIQHVYNVYGRDHAAMVCNIIRYRPKSAVRDVGKALGIPETALDRAAKH
                     LSHYGSVEADALARSGLAEGGHASALDHLARLSDEILEFPRHLSVHPGGFLLGHEPVH
                     DIVPIENAAMPGRTVIQWDKDDLEDLGLFKVDLLGLGALHQLHLGLDLMRKHRGLDLS
                     MATIPAEDEATYDMICTADTVGTFQIESRAQMSMLPRLRPRTFYDLVVEVSLVRPGPI
                     SGGMVHPYLRRRKGIEAVEYPHPSLETVLAKTLGVPLFQEQVMRLAIVAADYTPGEAD
                     QLRRDMAAWRRSGRIEKHRDRLIARMEEKGISREFSERVFEQIRGFGEYGFPECVVGD
                     TRVIDAETGAWVRIEDVVAGRVPLRYTLTCSDELKIEKRRVVAAKASGRKQVFKLRTA
                     LGREIEATANHPFLTMSGWTKLGDLKVGDAVATARALPSMGKKTWMKHELVALADLIA
                     EGNLCHPNTFYFYTQDDDHCQEFARSLERFPNTVAVVERHHGCFSVRARRIDRSQPAG
                     AVSWAKQLGMWGCGAYTKQIPDEVFQLCDRDIALVLARLWEGDGTIAVKGVHADYDTA
                     SRRLAADVQHLLLRLGIISRCYERTRPYRDRTVRSYVVTVTGAENLCTFYELIGQRFL
                     SVEKKRRAKLLAVGNDARMSKDVIPSEVHTLIRRARDERGLTWNAIGRATRLGMREIQ
                     SVSGGKRGFRRWVIGKLASHLRSPELERLATSDVYWDRIVSIEPAGEQETYDLSIDGN
                     HNFLANDFVVHNSHAASFALIAYATCWMRKHYLPEFTCSLLNAQPMGFYSPATIVGDA
                     QRHGLEIRPVDVTASDWECTLEPSGDDFRYAVRMGLRWLKGIQIAEGQKITEARRERA
                     FASVEDFVRRTHLPTRTHTTIAESGALGPLVSGRRDALWQVTGWVRRQDDPLDLGGDV
                     NDVGFAKLGTLDEIFWDYRASDHSTRGHPLAPMRDELRSQRLPDARTVSRGRDGQRIE
                     YVGVVICRQQPGTASGVTFMTLEDETGFVNLVVWAQVFAQYASIIRTTSLLGVTGRLQ
                     VQEGIVHLIAEKVWQPELSRPIAEVESRDFH"
     gene            3624..4553
                     /locus_tag="VFV99_32200"
     CDS             3624..4553
                     /locus_tag="VFV99_32200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064098.1"
                     /translation="MPRAPRLIVPGFVYHLISRFVDREWFITHERERRYYVSLLGRAI
                     SKSDWRCIGYAVMSNHVHISAVAGVHSLDSWIRRVHSPFADFMNKQHGRIGPLFVRGP
                     RAFETPASDVARLVAYIHNNPVRANVVANPADTRWTSHRAYLGLDPAPSWLDVRLGLR
                     LCGFDKPDAFAEFVALNPSDRARESLESGSVELDESALFREAIAEAPRRANESPSARS
                     VVEATALELQIPISSFCSRRRTATERLAREVAVHCASTVGISGVEIAHALRLSQPSAS
                     RALQRAVTRPDVIELSVNVQQRLRERATHTSNV"
     gene            complement(4818..5834)
                     /gene="dctP"
                     /locus_tag="VFV99_32205"
     CDS             complement(4818..5834)
                     /gene="dctP"
                     /locus_tag="VFV99_32205"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5064099.1"
                     /translation="MSKLVKLVSIAALAAVASPASLAFGDNTELRIATLAPSGSPWME
                     VLGKGQAEINTKTAGRLTVKYYEGGQQGDERDFVRKMGLGQLDGAAITSVGLAMVDES
                     IRVLELPRMFASVEELDAVADKMWPYFQKKFEKKGYKLADRGEVGWIYVMSKSKVDSV
                     ATLKSQKMWLWGDDQIFSALYKKLGLNGVPLGVPEVDAGLTSGKINGCYGSPVAAVAL
                     QWYSKVKYMTSMPMSFAIGATVIKMDSLKKASAEDQKAFDTIGKATQKKLRKVIRKAN
                     ADAQLTMTKKGVTVVETPAAMVAEFDKAAQEVWKDLAGKVYSKEELDMVIKYRDEFRA
                     KKKK"
     gene            complement(6015..7049)
                     /locus_tag="VFV99_32210"
     CDS             complement(6015..7049)
                     /locus_tag="VFV99_32210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064100.1"
                     /translation="MRAGGQARLAYADGARASAEQWASACIPFLTMEFGHHLEWDVTP
                     ALLAPLGLRAGANRRETVTFHWQPLKAKFGHLLAAVEAGEAQKQGITVTDPIDPENPS
                     FPQGDLVIFDVRLDATLLWSSGQPIASRQTPEATPFGYVRRQLAPWGEARDITVEVGS
                     GGGDFIDNGASVHVWGVRIENLKLGPLFTTGGIGITSGGVGDFVDDVSREVEVTTPRA
                     VLGVVTGGSALHGHVTATHDLELQPDGYVTVDSRITGGLGVSIGKTRALLDAAIAKTH
                     VYVPKTMATSAVTGGASLGVSQHLMPHLDATLRIDAARSFYAANVTSLDFVPRWGVTA
                     FAALQATVGH"
     gene            complement(7354..7818)
                     /gene="bcp"
                     /locus_tag="VFV99_32215"
     CDS             complement(7354..7818)
                     /gene="bcp"
                     /locus_tag="VFV99_32215"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX5064101.1"
                     /translation="MVEVGKKAPAFTLESSDGGKVKLGDLKGNVVVLYFYPRDNTPGC
                     TIEAENFRDAIPALKKLGAVVLGVSKDSIASHCKFRDKYKLNFPLLTDADGAVMEAYG
                     AWGDKVMYGKKMKGIIRSTVVIDKTGKVSHHWPKVSVKGHVDEVVEAVKALK"
     gene            7842..8333
                     /locus_tag="VFV99_32220"
     CDS             7842..8333
                     /locus_tag="VFV99_32220"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX5064102.1"
                     /translation="MRLLACLFLVYGLGLLGCQHQDAAQRSAEITAQATPKPASSSPP
                     INNALTMPAAKSACGCSGGGESCGGSCGGSCGASCGHGGGTDPLWAPLPSNSTWTPLQ
                     VAGMRCGGCAKRIERALASVEGVLGVKADVRMARIEIATKPGIDARALVKPTIDGLGY
                     QVQ"
     gene            8352..8681
                     /locus_tag="VFV99_32225"
     CDS             8352..8681
                     /locus_tag="VFV99_32225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064103.1"
                     /translation="MGRLLIVVASGALAGAGACGHSNASGPKWPEPSKTAEDGGESIE
                     PRPSASYAAAVEKSDDNDEDKPAASSTSSPASSTSEDKPATTSTPTTPTSEDVFQTDE
                     IIIEIDD"
     gene            complement(8751..9755)
                     /locus_tag="VFV99_32230"
     CDS             complement(8751..9755)
                     /locus_tag="VFV99_32230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064104.1"
                     /translation="MRTLGITFVLVLAAACGTDPAADDDGMGSGSGSGSGSGSGSDTL
                     DPPARGFQIVSPEITINAGQEITYCYYFKTPNTEAFTIKRWVSKMTGGSHHMILFFTP
                     SLGQPEGTISSSQCGLSGGGSNIPSWIYAAQNIDSDMPLPADDGSGKPLGMDVAPGQP
                     AFIQMHYFNTTDEAIKAHVTINAEAYDAGVATTKTAAYVTFNPSISIPAAQGTTPGTA
                     TATNTCNIPASSKVWLMSTHAHKQAIHTEVRDGAATSTNIVFQSDDWEHPGTKTWMTN
                     PFYTFASGKLTYECKYSNPNGYPISTGDSAATDEMCMATGFVFPATKATICYGNFIVP
                     "
     gene            9912..11186
                     /gene="gorA"
                     /locus_tag="VFV99_32235"
     CDS             9912..11186
                     /gene="gorA"
                     /locus_tag="VFV99_32235"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5064105.1"
                     /translation="MSRFDVDLFVIGGGSGGVRAARVAAEHGAKVAIAEASRWGGTCV
                     IRGCVPKKLLVYASEISRNLDDARGQGWTIANASFDWPTFRAAKDKELARLEQAYAQR
                     LQRAGVRLLQGHARLADPHTIEINDERITAAHVLVATGGKPRRPAGNYITSDEAFDLP
                     ELPKRIAILGAGYIGVEFAHIFAGFGVDVSLVHRGEHVLRGFDPELAAEVERGITAHG
                     IKLIAGDTIDIPTDLKMAAIGREPVSKDLGLVEAGIALDAHGAVVVDAFSRSNVPHIY
                     AVGDVTARYQLTPIAIREGHAVADTLFGNRPTAIVHELIPTAVFAQPPAAAIGLTEPA
                     ALADGHDIQVFRARFRPMRYSLSGRDEHVMIKLIVARATDKVLGLHIVGPDAPEIVQA
                     TAIAITMGATKADLDRTFALHPTTAEELVLLR"
     gene            11316..11879
                     /locus_tag="VFV99_32240"
     CDS             11316..11879
                     /locus_tag="VFV99_32240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064106.1"
                     /translation="MRAALVVVAALVAVPAFADTPQAVRGETIIIHGKAPPARLPKAK
                     KRYNRIAPAYSDYAVEHDRWAKAWLLLDIDERGVVTRLKLLKKPGMDLDQIAIDRGFS
                     MRFEPAEDINGNPMRTELITPIEWPAYWWLIDREGLATKVPEYIEKVPCYGSGPMHMG
                     SIHPMYRDCSPPPALTTLDEYPWITRK"
     gene            complement(11928..12473)
                     /locus_tag="VFV99_32245"
     CDS             complement(11928..12473)
                     /locus_tag="VFV99_32245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064107.1"
                     /translation="MQRLLNSSRMRFTPSIALALIALALIACGNDVRNSDDDDNVEPD
                     AGAGGSVVADAMPVPPDGIPPNLTPCEEAAYHSDLAFIQDKIFDVSCTTQCHGDSPPA
                     AQMSLLPGHARVALINVASTTQTGWRRVVPGSSASSLLMVEIGGESGPALEGLMPWGM
                     PKLCDEKIDAIRRWIAAGANP"
BASE COUNT         2178 a         4120 c         4096 g         2310 t
ORIGIN      
        1 tgcgcaagct cgcgttcgac ggcgcggcgt ggcgctacga cggcgacatc ccggcgaacg
       61 tgcgcgcgca gctcgatgcc gagctcgccg tcatcgagga gctcgactac ccgggctact
      121 tcctcacgat gtacgagatc gtgtcgtact gccggcggcg cgacatcatg tgccagggcc
      181 gcggctcggc cgcgaactcc gccgtgtgct tctgcctcgg catcaccgcg gtcgatccca
      241 ttcgcatggg cctgctgttc gagcgcttcc tgtcgcgcga gcgcgccgag ccgcccgaca
      301 tcgatctcga catcgagcac gagcgccgcg aagaagtgat ccagcacgtc tacaacgtct
      361 acggccgcga tcacgccgcg atggtttgca acatcatccg ctatcgcccg aagtccgccg
      421 tccgcgacgt cggcaaggcg ctcggcatcc cggagaccgc gctcgatcgc gccgccaaac
      481 acctttcgca ctacggctcc gtcgaggccg acgcactcgc gcgctccggt ctcgccgaag
      541 gtggccacgc ctccgcgctc gatcacctcg cgcgcctctc cgacgagatt ctcgagtttc
      601 cccgccatct ctccgtccat cccggcggct ttttgctcgg tcacgagccg gtccacgaca
      661 tcgtgccgat cgaaaacgcg gcgatgcccg gccgcaccgt catccagtgg gacaaggacg
      721 acctcgaaga cctcggcctc ttcaaggtcg atctgctcgg cctcggcgcg ctccaccagc
      781 tccacctcgg cctcgatctc atgcgcaagc atcgcggcct cgatctctcg atggccacga
      841 tccccgccga ggacgaagcc acctacgaca tgatctgcac cgccgacacg gtcggcacgt
      901 tccagatcga gtcgcgcgcg cagatgtcga tgctgccgcg gctgcgcccg cgcacgttct
      961 acgatctcgt cgtcgaggtc tcgctcgttc gcccgggccc gatctcgggc ggcatggtcc
     1021 atccgtacct ccgccgccgc aaaggtatcg aggccgtcga gtatccgcat ccatcgctcg
     1081 agaccgtgct cgccaaaacg ctcggcgtgc cgctgttcca ggagcaggtg atgcgcctcg
     1141 cgatcgtcgc cgccgactac acgccgggcg aagccgatca gttacgccgc gacatggccg
     1201 cgtggcggcg ctcgggccgc atcgagaagc atcgcgatcg cctgatcgcg cgcatggaag
     1261 agaagggcat ctcgcgcgaa ttctccgagc gcgtcttcga gcagattcgc ggcttcggcg
     1321 aatatggttt cccagaatgc gtcgtcggtg acacgcgtgt gatcgatgcc gagaccggtg
     1381 cgtgggtgcg gatcgaggat gtcgtcgccg gccgcgtccc gttgcgctac acgctgacgt
     1441 gcagcgacga gctgaagatc gagaagcgcc gcgtcgtcgc cgcgaaggcg agcggccgca
     1501 agcaagtgtt caagctacgc accgcccttg ggcgcgagat cgaggccacg gcgaaccatc
     1561 cgttcttgac gatgtccggc tggacgaagc tcggcgatct gaaggtcggc gacgcggttg
     1621 cgacggcgcg cgctctgcct tcgatgggca agaagacctg gatgaaacac gagctcgtcg
     1681 cgctcgcaga cctgatcgcg gaaggcaacc tatgccaccc caacacgttc tacttctaca
     1741 cgcaggacga tgaccactgc caagagttcg ctcgttcgct cgagcggttc ccgaacacgg
     1801 tggcagttgt tgagcggcat cacggctgtt tcagcgtacg tgcacgccgc atcgatcgct
     1861 cgcaaccggc tggcgcggtc agctgggcca aacaactagg catgtggggc tgcggcgcgt
     1921 acacgaagca gatacccgat gaggtctttc aactctgcga tcgagacatc gccctggtcc
     1981 ttgcccgtct gtgggaaggc gacgggacga ttgccgtcaa aggtgtgcac gcggattacg
     2041 acactgcatc gcgcagactt gcggcggacg ttcagcatct gttgctccgg cttggaatca
     2101 tttctcgttg ctacgagcgc acgcggccgt atcgcgaccg aactgtccgc agttacgtcg
     2161 taaccgtgac gggagccgag aatctgtgca cgttctacga gctgatcggc cagcgttttc
     2221 tgagtgtcga aaagaagcgg cgcgcgaaac tgctagcagt cggcaatgac gctcggatgt
     2281 cgaaggacgt aattccgtcc gaggtccaca cgttgattcg tcgcgctcgt gatgagcgtg
     2341 gtctgacgtg gaacgcgatc gggcgcgcca caagactcgg catgcgcgaa atccagagcg
     2401 taagcggcgg caagcgcggt tttcgacgct gggtgatcgg caagctcgcg agccacttgc
     2461 ggtcgccgga gctcgaacgg ctagcgacct ccgatgtgta ttgggaccgc atcgtcagca
     2521 tcgagccagc tggcgagcag gagacgtacg acctctcgat cgatggcaat cacaacttcc
     2581 tcgccaacga cttcgtcgtg cacaactctc acgccgcaag cttcgcgttg atcgcgtacg
     2641 cgacgtgctg gatgcgcaag cactacttgc cggagttcac gtgctcgctg ctcaacgcgc
     2701 agccgatggg gttctactcg ccggcgacga tcgtcggcga tgcgcagcgg cacgggctcg
     2761 agatccggcc ggtcgacgtc acggccagcg actgggagtg cacgctcgag ccgagtggcg
     2821 acgacttccg ctacgcggtg cgcatgggcc tgcgctggct caagggcatc cagatcgccg
     2881 aagggcaaaa gatcaccgag gcgcggcgcg agcgcgcgtt cgcatcggtc gaagatttcg
     2941 tgcggcgcac gcacctgccg acgcgcacgc acacgacgat cgcggagtcg ggcgcgctcg
     3001 ggccgctggt cagcgggcgg cgcgatgcgc tgtggcaggt gacgggctgg gtgaggcgcc
     3061 aggatgatcc actcgatctc ggcggcgacg tgaacgacgt cgggtttgcc aagctcggca
     3121 cgctggatga aatcttctgg gactatcgcg cgagcgatca ctcgacgcgc ggccatccac
     3181 tcgcgccgat gcgcgacgag ctgcgctcgc aacgcttgcc ggatgcgcgc acggtgtcgc
     3241 gaggccgcga cggtcagcgc atcgaatatg tcggcgtcgt gatctgccgc cagcagcccg
     3301 gtaccgcgag cggcgtgacg ttcatgacgc tcgaagacga gaccggcttc gtgaacctgg
     3361 tcgtgtgggc gcaggtcttc gcgcagtacg catcgatcat ccggacgacg tcgttactcg
     3421 gcgtgaccgg gcgactgcag gtgcaggaag gcatcgtgca cctgatcgcc gagaaggtct
     3481 ggcaaccgga gctgtcgcgg cccatcgctg aagtcgagag ccgcgacttt cattagacgt
     3541 ccggcctacg gacagggtca gacatgcgtt ccatgcgttc tgccgcgcat cgcatgtcag
     3601 tacaactgac tacggtcacg cgcatgccgc gcgctccacg tctcatcgtc cctggcttcg
     3661 tctatcacct gatctcgcgc ttcgtcgatc gcgagtggtt catcacccac gaacgcgaac
     3721 gtcggtacta cgtatcgctg ctgggtcgag cgatctctaa gtcggactgg cgatgcatcg
     3781 gctacgcggt gatgagcaac cacgtgcaca tctctgctgt tgccggtgtg cacagcctcg
     3841 actcttggat tcgacgtgtg cactcgccat ttgccgactt catgaacaaa cagcacggcc
     3901 gtatcggccc gctgttcgtg cgcggtccac gcgcgtttga aacgccggcg agcgatgtcg
     3961 ctcgtctcgt cgcatacatt cacaacaatc cggtgcgcgc caacgtagtc gcgaaccctg
     4021 ccgacaccag gtggacgagt caccgcgcat acctgggcct cgaccctgcg ccgtcgtggc
     4081 tcgatgtcag gctcggttta cgcctgtgcg gattcgacaa gccagatgca tttgcagagt
     4141 tcgtcgctct taacccgagc gatcgtgccc gcgaatcgct cgagagcggt tccgtcgagc
     4201 tggatgaatc agccctcttc cgcgaagcga tagcggaagc tccaaggcgg gccaatgagt
     4261 caccgagcgc gcgatccgtc gtcgaagcaa cggctctcga acttcaaatc ccgatatcca
     4321 gcttctgttc gcgacgacgg acggccacag aacgacttgc gcgcgaggtc gccgtgcatt
     4381 gcgcgagcac cgtaggtatc tcgggcgtcg aaatagcaca tgcgttgcgt ctatcccaac
     4441 catcggcatc tcgcgcgctt cagcgcgcag tcacgcgccc ggatgtgatc gagctgagcg
     4501 tcaatgtcca gcagcggctg cgcgagcgcg caacgcatac aagtaatgtc tgaccccgtt
     4561 ctcccgttct cccccatggc agatcctctg cgtgcacatc gcgtgcggcc tccgtcgcgt
     4621 cgcgctcggt catggcgcat cacgggtgct cggcgatagc acggtggcga tcgggaccgg
     4681 tctgtccgtg gtcgtgtcgt gtctgatctt caccgcgctg gtccggtgat caaggccaga
     4741 cccgatgcac ccgtgcgcaa agcactactt gcgttcgcga gcttgccgca acgaaagcag
     4801 ccgcacgcgt tcactgttca cttcttcttc ttcgcgcgga actcgtcgcg gtacttgatg
     4861 accatgtcga gctcttcctt cgagtagacc ttgccggcca gatctttcca gacctcctgc
     4921 gcggccttgt cgaactcggc gaccatcgcg gccggtgtct cgacgacagt aacgcccttc
     4981 ttggtcatcg tgagctgcgc gtcggcgttc gccttgcgga tcaccttgcg cagcttcttc
     5041 tgcgtggcct tgccgatcgt gtcgaacgcc ttctgatcct cggccgaggc cttcttgagc
     5101 gaatccatct tgatgacggt ggcgccgatc gcaaagctca tcggcatcga cgtcatgtac
     5161 ttgaccttcg agtaccactg cagcgcgacg gcagcgactg gcgagccgta gcagccattg
     5221 atcttgccgc tcgtgagacc ggcatcgact tcgggcacgc cgagcggcac gccgttgagg
     5281 ccgagcttct tgtagagcgc gctgaagatc tgatcgtcgc cccacagcca catcttctgg
     5341 ctcttcagcg tcgcgaccga gtcgaccttc gacttcgaca tgacgtagat ccagccgacc
     5401 tcgccgcggt cagcgagctt gtagcccttc ttttcgaact tcttctggaa gtagggccac
     5461 atcttgtcgg cgacggcgtc gagctcttcg accgacgcga acatgcgcgg cagctcgagc
     5521 acgcggatcg actcgtcgac catcgcgagg ccgaccgacg tgatcgccgc gccatcgagc
     5581 tgaccgaggc ccatcttgcg cacgaagtcg cgctcgtcac cttgctggcc gccttcgtag
     5641 tacttgaccg tgaggcggcc ggcggtcttc gtgttgatct ccgcctggcc cttgccgagg
     5701 acttccatcc acgggctgcc cgagggcgcg agcgtcgcga tgcgaagctc ggtgttgtcg
     5761 ccgaaagcga gagacgcggg ggacgcgacg gcggcgagtg ccgcgatcga aacgagttta
     5821 accagcttgc tcatgagacc gttcctcgaa gaatgatgtc gaacgatgtg ccggcgcggc
     5881 ggccttgtcc agcgttgaat gcgacgcgcg gcatcgggcc gtattcacat cgtcgtcggg
     5941 ccgtattcac gtaaaggcgc cttacatcca cagcgcgcaa atccaagggc ggcaagcgga
     6001 gtagccagtg tcgcttagtg accgacggtc gcttgtagcg ccgcgaacgc agtcaccccc
     6061 cagcgcggga cgaagtcgag cgaagtcacg ttcgccgcgt agaacgagcg cgcagcatcg
     6121 atgcgcagcg tcgcgtcgag atgcggcatg aggtgttgcg agacgccgag tgatgcaccg
     6181 cctgtgaccg cgctcgtcgc catcgtcttc ggcacgtaga cgtgcgtctt cgcgatcgct
     6241 gcgtcgagca gcgcgcgtgt cttgccgatc gagacgccga gaccaccggt gatgcgcgag
     6301 tcgaccgtga cgtagccgtc tggttgcaac tcgagatcgt gcgtcgccgt cacgtgcccg
     6361 tgcagcgccg agccaccggt cacgacgccg agcacggcgc gcggcgtcgt gacttcgact
     6421 tcacggctca cgtcgtcgac gaagtcgcca acgccgcccg acgtgatgcc gataccaccg
     6481 gttgtgaata acgggccgag cttgaggttc tcgatgcgca cgccccagac gtggaccgac
     6541 gcgccattgt cgatgaagtc gccgccaccg gagccgacct cgacggtgat gtcacgcgct
     6601 tcgccccacg gcgcgagctg acggcgcacg taaccgaacg gcgtggcctc gggcgtctgg
     6661 cgcgacgcga tcggttgacc cgaggaccac aggagcgtcg catcgaggcg cacgtcgaag
     6721 atcacgaggt cgccttgggg gaagctcggg ttctcgggat cgatcggatc ggtgacggtg
     6781 atgccttgct tctgtgcctc gcccgcttcg accgccgcga gcaggtggcc gaacttcgcc
     6841 ttcagcggtt gccagtgaaa cgtcaccgtc tcgcgccgat tcgctcccgc gcggagcccg
     6901 aggggcgcga gtaacgcagg tgtgacatcc cattcgagat ggtgtccgaa ttccatcgtc
     6961 agaaacggga tgcacgcgct cgcccattgc tcggcgctcg cgcgggcgcc atcggcgtag
     7021 gcaaggcgcg cttggccgcc tgcgcgcacg gggtcgcaac gcgtgccgcg ataccggatc
     7081 tcgccgccgg ccgcgaccga accttcggcg cgtcggccct tccgccgacg agctgggcga
     7141 acgccgccgc gtggaagccg ttgtccgtga cctcgggcaa cgcaagcgca cgcagctcgg
     7201 tttcgaccgt ttcggaatcc gcggctgcga tggtgggaac ggcgagcacg aacacgaacg
     7261 gaactgcgcg gcgcattcgg gcacgtcaga gcaattcgct cgccagcgga attcgcggca
     7321 accggtggca ggagcgtccg acttcgaacg cgactacttc agcgctttga cggcttcgac
     7381 gacttcgtcg acgtggcctt tgacgctgac cttcggccag tgatgcgaga ccttgcccgt
     7441 cttgtcgatg acgacggtcg agcgaatgat gcccttcatc ttcttgccgt acatcacctt
     7501 gtcgccccac gcgccgtagg cctccatcac cgcgccgtct gcatcggtca gcagcgggaa
     7561 gttgagcttg tacttgtcgc ggaacttgca gtgcgacgcg atcgagtcct tgctcacgcc
     7621 gagcacgacc gcgccgagct tcttcagcgc cgggattgcg tcgcggaagt tctccgcctc
     7681 gatcgtgcaa cccggcgtgt tgtcgcgcgg atagaagtag aggacgacga cgttgccctt
     7741 gagatcgccg agcttgacct tgccgccgtc ggaggactcg agcgtgaacg ccggtgcttt
     7801 cttgccaact tcgaccatgg cggtgtgata cacgaattgg gatgcgcttg ctcgcctgcc
     7861 tcttcctcgt ctatggcctc ggcctcctcg gctgtcagca ccaggatgcc gcccagcgct
     7921 cggccgagat caccgcgcag gcgaccccga agccggcgtc gtcatcgccg ccgatcaaca
     7981 acgcgctcac gatgcccgcc gcgaagtctg cctgcggctg ctcgggcggc ggcgaaagct
     8041 gcggcggttc gtgcggcgga tcgtgtggcg cttcgtgcgg ccacggcggc ggtacggatc
     8101 cgctgtgggc tccgctgccg tccaactcga cgtggacgcc gttgcaagtc gcggggatgc
     8161 gctgcggtgg ctgcgcgaag cgtatcgagc gcgcgctcgc cagcgtcgag ggcgtgctcg
     8221 gcgtgaaggc cgatgtacga atggctcgga tcgaaatcgc gacgaagccg ggcatcgacg
     8281 cccgcgcgct ggtcaagccg accatcgacg gcctgggcta tcaggtccag tagctgctac
     8341 gcttccagac gatgggtcgt ctgttgatcg tcgtcgcttc gggtgcactg gccggtgccg
     8401 gcgcatgcgg ccattcgaac gcgtccggac cgaagtggcc cgagccgtcg aagaccgcgg
     8461 aagacggtgg cgagagcatc gagccgcgcc cgagcgcgag ctacgcggcg gcggtcgaaa
     8521 agtcagacga caacgacgag gacaagcccg cggcgtcgag cacgagctcg cctgcctcga
     8581 gcacgagcga ggacaagccg gcgacgacgt cgacgccgac gacgccgacg tcagaagacg
     8641 tgttccagac cgacgagatc atcatcgaga tcgacgactg aggccgagcg atcgccgaaa
     8701 cggaaaaagc cgcgtatcgc tacgcggctt ttcttacgtc gtcgacgcgc ttacgggacg
     8761 atgaagtttc cgtagcagat cgtcgccttg gtcgccggga agacgaagcc cgttgccata
     8821 cacatttcgt cggtggcggc gctgtcgccc gtgctgatcg gatatccgtt cgggttgctg
     8881 tacttgcact cgtacgtcag cttgccgctg gcaaatgtat agaacgggtt cgtcatccag
     8941 gtcttcgtgc cgggatgctc ccagtcgtcg ctctggaaca cgatgttcgt gctcgtcgcg
     9001 gcgccgtcgc gcacctcggt gtggatcgct tgcttgtgcg cgtgcgtcga catcagccac
     9061 accttcgacg acgccgggat gttgcaggtg ttcgtcgccg tcgcggtgcc gggcgtggtt
     9121 ccctgcgcgg ccggaatgct gatgctcgga ttgaacgtga cgtacgcggc ggtcttcgtc
     9181 gtcgctacac ctgcatcgta ggcctcggcg ttgatcgtga cgtgcgcctt gatcgcctcg
     9241 tccgtggtgt tgaagtagtg catctggata aacgcgggtt gacccggcgc gacgtccata
     9301 ccaaggggct ttccgctgcc gtcgtcagcc ggcaatggca tatcggagtc aatgttctgc
     9361 gccgcgtaga tccacgacgg aatgttcgag ccgccgccgc tcaggccgca ttggctcgac
     9421 gaaatcgtac cttcgggttg gccgagtgac ggcgtgaaga agaggatcat gtggtggctg
     9481 ccaccggtca tcttcgagac ccagcgcttg atcgtgaacg cctcggtgtt gggcgtcttg
     9541 aagtagtagc agtacgtgat ctcttggccc gcgttgatcg tgatttcggg gctgacgatc
     9601 tggaaaccgc gcgcgggcgg gtcgagcgta tcgctgcccg atccggaacc cgagccggag
     9661 cccgagccgc tgcccatccc atcatcgtct gccgctggat cagtgccgca cgcggcggcg
     9721 agcacgagca cgaacgtaat acctagtgtg cgcatgggac gggccccctc tggccaaacg
     9781 tttgtggagc gagggtatca aggcggtcga cggtgtctag ggtttattga attcaagttc
     9841 cattacctgt tcgtcttcac ctgacaagct gtgatgccag tgtgcggcag cccacgtaca
     9901 ctgcagacct catgtcgcgc ttcgacgtgg atctgttcgt gatcggtggt ggctcgggag
     9961 gcgttcgagc cgcacgcgtc gctgcagaac acggggccaa ggtcgcaatc gcggaagcga
    10021 gtcggtgggg cggtacatgc gtgatccgcg gttgtgtgcc gaagaagctg ctcgtgtacg
    10081 cgagcgagat cagtcgcaat ctcgatgacg cacgcgggca aggttggacc attgcgaatg
    10141 cgagcttcga ctggccgacg ttccgcgcgg ccaaggacaa agagctcgcg cgactcgagc
    10201 aagcctatgc acaacggctc cagcgggccg gcgttcgctt gttgcagggc catgcacggc
    10261 tcgccgaccc gcacacgatc gagatcaatg acgagcggat tacggccgcg catgtcctgg
    10321 tcgcgaccgg tggcaagccg cgacggccgg ccggtaacta cataacgagc gacgaggcgt
    10381 tcgaccttcc cgagctgccg aagcggatcg cgatcctcgg cgcgggctac atcggcgtcg
    10441 agttcgcgca catctttgcg ggctttggcg tcgacgtgtc gctcgtgcat cgcggcgagc
    10501 acgtgctgcg cggcttcgat cccgagctcg ctgcggaggt cgagcgcggc atcacggcgc
    10561 acggcatcaa gctcatcgcc ggcgacacga tcgacattcc gaccgatctg aagatggcgg
    10621 cgatcggtcg cgagccggtg agcaaggatc tcgggctcgt cgaagcgggc atcgcgctcg
    10681 atgcgcatgg cgcggtcgtg gtcgatgcgt tctcgcgctc gaacgtgccg cacatctatg
    10741 ccgtcggcga cgtgaccgcg cgctatcagc tgacaccgat cgcgattcgc gagggtcacg
    10801 cagttgcaga tacgctgttt ggcaatcggc cgactgcgat cgttcacgag ctgattccga
    10861 cggccgtgtt cgcgcagccg cccgctgctg cgatcggact gaccgagccc gccgcgctcg
    10921 cggacggtca cgacatccag gtgttccgcg ctcggttccg gccgatgcgt tactcgctgt
    10981 cgggccgcga cgagcacgtg atgatcaagt tgatcgtcgc acgcgcgacc gacaaagtgc
    11041 tcggcctgca catcgtcggg cccgatgcgc cggagatcgt ccaggccacg gcgatcgcga
    11101 tcacgatggg cgcgacgaag gccgacctcg accgcacgtt cgccctgcat ccaacgacgg
    11161 ccgaagagct cgtgctgttg cgttgaccga cgctgacagc gtgcgagctg tgagctttcg
    11221 gcccgtgctg tggttctgtg gcaacagcga gccggtccgg tttgtgcaaa atcgcgcgtc
    11281 tagtgctggt gcacgacgtg cagaagcgca ctgccatgag agccgcactg gttgttgtcg
    11341 ccgccctcgt cgcggttccc gcgttcgccg acacgcccca ggcggtccgc ggcgagacca
    11401 tcatcatcca tggcaaggca ccgccagcac ggctgccgaa agcgaagaag cgctacaaca
    11461 ggatcgcgcc ggcctacagc gactatgctg tcgagcacga ccggtgggcg aaggcgtggc
    11521 tgctactcga catcgacgag cgtggcgtcg tgacgcggct caagctgctc aagaagccgg
    11581 gcatggatct cgatcagatc gcgatcgacc gtggcttcag catgcgcttc gagcccgccg
    11641 aggacatcaa tggcaacccg atgcgcacgg agctgatcac accgatcgag tggccggcgt
    11701 actggtggct gatcgatcgc gagggcctcg cgacgaaggt gcccgagtac atcgagaagg
    11761 tgccgtgcta cggcagcggc ccgatgcaca tgggctcgat ccatccgatg tatcgcgact
    11821 gctcgccgcc gcccgcgctc acgacgctcg acgagtatcc ttggatcacg aggaagtaga
    11881 aaacgcggtc agacccatat cccgcgagga agcagaagaa ggggctgcta cggattcgca
    11941 cccgctgcga tccagcgccg gatcgcgtcg atcttctcgt cgcacagctt cggcatgccc
    12001 catggcatca ggccttcgag cgccggaccg gactcgccgc cgatctccac catcaacaag
    12061 ctcgacgccg agctgccagg cacgacgcgc ctccatccgg tctgcgttgt cgacgctacg
    12121 ttgatcagcg caacgcgcgc gtgtcccggc aacaggctca tttgtgccgc cggcggtgaa
    12181 tcgccatggc actgcgtcgt gcacgacacg tcgaagatct tgtcctgaat aaaggccagg
    12241 tcggagtgat aggcggcctc ttcgcacggc gtgagattcg gcggaatacc gtcgggcggc
    12301 actggcatcg catccgcgac gactgacccg ccggcgcccg cgtcgggctc cacgttgtcg
    12361 tcatcatcgg agttgcgcac gtcgttgccg cacgcgatga gcgccaacgc gatgagcgcc
    12421 aacgcgatac taggggtgaa tcgcatgcgc gacgaattca gcaagcgctg caccagcagc
    12481 gactcgcgga tcgtcgagac ttagcggctt gcgcttcgtg cggtgctcgc tgcggcgcgc
    12541 tcccacgcac ggcgtgcgcg cggaacattg tgtttcgtta gatcacggac agaccgggta
    12601 acaccggccc cacggctcgt tcgtgccgat gatcgggcag cacattgccg cttgcgccgg
    12661 acaatccgcg ttgtcggtgc agatcgtcgc gttggtgcac gtgg
//
LOCUS       DASUFX010000798        11592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1934570, whole genome shotgun sequence.
ACCESSION   DASUFX010000798 DASUFX010000000
VERSION     DASUFX010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934570"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3062)
                     /locus_tag="VFV99_32250"
     CDS             complement(<1..3062)
                     /locus_tag="VFV99_32250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5064108.1"
                     /translation="MSGGRSYLAGFAALHTLATADERRQATRQFLAALAAISEREPAP
                     LEGISADQLLSAVRAAFGDGLLTSLDWLSPASAAIALFELAQALPPGAERRELGRRVL
                     TSLRAADRATFVRLLIALARTSPKIVAADHLRARLSVVLAAPFTAPGMIGELAIGLLA
                     QPELAHSWVEEPATGSLPGRRLAARILAHGAREAVRRHDAGDRGGVRVLARPGVRAAL
                     ARLLGDREALVSRFASIARGILAHVDPDLAGEIDRELRPAASNTEVRRGAASAAAALE
                     RGGAARRWHDMLIARAAKEPGVGRGAILGLAGLAIASPAEADELATKLVARQPLECAE
                     ALAELRREEAGTLLPEAEAAALDWTRAQMKADQDPKDDGRWALLHALEAELGGIVVTE
                     GIGVPIGEARTALDDGDTQGALRAARTAVNEISDAAEWLKGASDDDPIDRRHSMRLLR
                     ELDRELLADNALPAVLALAGDADPARQQFARALAAIEAALLAHERAPESGAVAHGGLR
                     IARLRALVRLLDGVRVTGDADLEPRLAAVRQLMARAEVDRSPLRRAVWAAMTRAGDAL
                     LRDGHAELTDLLFAWTTAFPDDDFAIVRDASMVPEVEQSFQAYAALQQATWEAADPDH
                     AEALRVVVERLGELTDALPPEQSPRVESVRLALARLGNQLTRLTSVSSQQAVSAGTLD
                     AIATELGALARRSLGARRRLGIDAEDHAGSLETAVRGIEAVLATDQRDARSAALDEAI
                     AASIDESRAALPPALAAAIERVLVWLARRPIDRSESTTATADVTLPGWVPLSRMLGGY
                     YVVRPIGRGAGGSVLLAVRADERTRADRELVAVKVPDYSGDAARELSEREFVRLFREE
                     AGALLALPEHPNIARFITFDASAQPKPILVMEFVRGTNLERTLEAGELTMDRALSIID
                     DLLRGLEAMHAARIAHLDVKPPNLVLREGSGDAVLVDFGLAGRHIRAGCGSPHYGAGE
                     VWTEDPSKLDPYPADVYAAACVAYEVLTGQLLIAGDTLKEVIDQHFSK"
     gene            complement(3083..4360)
                     /locus_tag="VFV99_32255"
     CDS             complement(3083..4360)
                     /locus_tag="VFV99_32255"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5064109.1"
                     /translation="MNRVGLTRRDVLRIAGIGSASLIVGCGDNERPRDAGNSHAAFVV
                     EPDESSFIVVVWSSEARTVALEVQSGDAIVMSTVMEISEIGSFDVTGLAASTNYQVHL
                     VFDTGVELGPHHVRTAPLADDPRPVRIAVSADVDPNLEFDSELLAHCAAASPEVFVSI
                     GDFPYADNGPDVAVTVDAYRNRYAQTVTAPRQRAWLQAMAVRAIYDDHEFRNDFDGMY
                     RVAEASRYAAAMQVWDEFFPVRGAAGEIRYRAFRWGAHVDCIMLDCRRFRSANAAPDD
                     ANKTMLGDTQRAWLIDTLESSTATFKLVFTSVPLDFADGNDHWAAYTYERSLIFDALV
                     GIPGILFVSADQHWFAAHKHAHGIREFQVGPVCRGIGTPHVFAPGVLFRSMQYNFALI
                     DVDGEQLTFSGVGPDGVTFYKETLTPQALTPQP"
     gene            4400..5311
                     /locus_tag="VFV99_32260"
     CDS             4400..5311
                     /locus_tag="VFV99_32260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064110.1"
                     /translation="MMRASLVLAFCLAACAFEHGTDPTTGGADPGSGSGSGDPDQDTD
                     GDGNTDLTDNCSGVSNPDQHDHDDDGRGDACDVCPHLVDTGKDTDGDGVGDACDPRPT
                     EPGDRIAFFEGFYDAPAWTSVIGANTWQMESGTLRQHGLDAAYQLVRDDNPDLGSVLV
                     DARVKINAISNNGSMRRSTGIVMSYRDANHYIFCGLAAPQSSQQGAEVNAGQVSTDFW
                     GSAQYNYAPGVFPAQMAGDWLTMQARTTKTDWGTTQIDCVTHRAGTTGTATYDGDAEI
                     DGDIGIRTNGADASFDYVFVVASPAAT"
     gene            5398..5925
                     /locus_tag="VFV99_32265"
     CDS             5398..5925
                     /locus_tag="VFV99_32265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064111.1"
                     /translation="MREASRLPQPSIVDIDPEEPTQLLDDVGQYSVIDLDLGVEAQPH
                     AEADALIDAAIDEAEGGEAEEEDELEARSRDEAEVPMKDKDTGDLYGVHQPPATDRQL
                     DKTANQEVFLDADQGENWLETLGHKSVEYGPETEHQVDVIDDSDEHLDHRGHHASESG
                     DRPVADKGSGGDGGL"
     gene            5985..6686
                     /locus_tag="VFV99_32270"
     CDS             5985..6686
                     /locus_tag="VFV99_32270"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5064112.1"
                     /translation="MKIERLDIAGLTTRVVGNGKQTVVLLHGFGAPGDDLVALAQYID
                     APARFVFPEAPLELGGLYGDSRAWWQLDLMRLEQELSSGKPRDRRGEVPEGLSDARDH
                     MVRFLEELEERFAIENSQLVLGGFSQGAMLALDVALHREVAPAGLILMSGTLLAESIW
                     QPLMSKLSGVPVFQSHGMHDQLLPFSIARQLRDQLKAAGARVDWCEFHGGHEIPPSVL
                     DGATKLLLTTGSMSR"
     gene            complement(6664..7431)
                     /locus_tag="VFV99_32275"
     CDS             complement(6664..7431)
                     /locus_tag="VFV99_32275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064113.1"
                     /translation="MKHLAALLVVVGCASGRSNVSGTDGGDIIDPRVDGQIEGQVDAA
                     LADAKPPVDAQPIDAFVPPDAPPPPPDASVQMPDAYQCTVMTRQLLTNPVLDLTPVGT
                     GWTLVNIDNAYPVVTDQGLAAHTAPYKAWMGGFEAPLGQSVTDQLYQDVTVPMGTTQL
                     TLTGYYVVGTQETTTTSVYDSGQLGLTQTNGTPIQTVLSLSNLTTTGATWTAINFTFP
                     QNLSGQTVRLRMTSTNDDSFVTNFFFDSFALTATYCQ"
     gene            complement(7557..8924)
                     /locus_tag="VFV99_32280"
     CDS             complement(7557..8924)
                     /locus_tag="VFV99_32280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5064114.1"
                     /translation="MSALVTTSDVSLTWSDRPGRALLRLAWPITVSMVSFSAMTLAST
                     AFVAHVGADELAGVGLAGVVGFTLVCFGIGLLRGAKTLVSQAVGANRLDKIPELVGAA
                     LCIAIVLGVLATVTGQLVAPLLAHLSASPRAGHFAAQYLGIRSLGAPLVLVYAALRES
                     RYGIGDTRAPMRASLAGNAVNIALDIVLILVLDWGVRGAAYATIAGNATELAVLAWPM
                     RAQLKKLGWSRKAARDVWAQGVPNGLQFIMEVGSFLILTVLVARMSAVDAAAHQMVLH
                     LVNVSFLPAHALAEAAAVLVGQAVGAGKDVLVPRVARRALAIGAGYSTVVLVGYAILG
                     GTIATAMASGDSALAERATLLVYVGLAFLVADAANVIARGVLRGASDVRFAAVVGIIT
                     SWLTTPPLTWLLGVHLGLGVVGGWVGLAVEIVLGASLFWLRVLRGGWRPAAAAARRAM
                     AGTAV"
     gene            9409..10380
                     /locus_tag="VFV99_32285"
     CDS             9409..10380
                     /locus_tag="VFV99_32285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5064115.1"
                     /translation="MSEDTAKLQSQFTTLKSEVGKVLVGQDDMLHRLLLGLLAGGHVL
                     LEGVPGLAKTLVIRTLADALDGTFSRIQFTPDMLPGDVVGTQIYNPREGTYTVKKGPV
                     FGNFILADEINRAPAKVQSALLEAMQERQVTLGDQTFKLADPFLVLATQNPIEQEGTY
                     PLPEAQLDRFLLKVKVGYPTKDDEVKILDRMAGAGAEPHASKVMTCDEILAARDKVKQ
                     VFVDDKLKRYTVDIVAATRDPKAAGLGALAPLIDNGASPRASIALIKVAKAHALLSGR
                     DYISPHDIKTIAHDVLRHRLLVSYEAEAQGKTTDHVVAQILENIPVP"
     gene            10387..11358
                     /locus_tag="VFV99_32290"
     CDS             10387..11358
                     /locus_tag="VFV99_32290"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5064116.1"
                     /translation="MLPAEIAQAVKRIQFVTGRQVADVMAGAYLSVFKGRGMEFDEVR
                     PYVPGDDVRSIDWNVTARTGEPHVKRFVEERELTVMLLVDVSASQDFGSGRRSKLEAA
                     VELSALLALSAVENGDKVGMLLFHGGADLYIPPRKGGKHALRVVREVLARGNDIATGP
                     AKRELLPRFVRRWFKLLQDKTRTEPRRSTSIAAALEFCRQVLPRRAVLFLISDFLDDG
                     YIQVLRHANRKHDVVAALITDPREADMPPAGLVTLEDAESGKLRLVDTRSKAFRDDLA
                     TRAATRQKELHDQLRATGMDLVTFDASGSMVDPLLKFFRERERRLRR"
     gene            11355..>11592
                     /locus_tag="VFV99_32295"
     CDS             11355..>11592
                     /locus_tag="VFV99_32295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064117.1"
                     /translation="MKRLLVIGALAACGGTSMLDQSQPAKSEPTTDALKKTTENGPVK
                     ATVTVWPPKPTLGEPIYFRLDVDAPAGISVDAPFQ"
BASE COUNT         2058 a         3849 c         3884 g         1801 t
ORIGIN      
        1 tgcttcgaga agtgctgatc gatgacttct ttcagcgtat cgcccgcgat cagcagctgg
       61 ccggtgagca cctcgtacgc gacgcacgct gcggcataga cgtcggctgg atatggatcg
      121 agcttcgacg gatcctccgt ccacacttcg ccggcaccgt aatgcgggct tccacaaccg
      181 gcgcgaatat gtcgtcccgc gaggccgaaa tcgacgagca cggcatcacc tgaaccttca
      241 cgcaacacca gattgggtgg cttgacgtcg agatgtgcga tgcgagctgc gtgcattgct
      301 tcgaggccgc gcagcagatc atcgatgatc gagagcgcgc gatccatcgt gagctcgccg
      361 gcttcgaggg tgcgctcgag gttcgtaccg cggacgaact ccatcacgag gatcggcttg
      421 ggttgtgcgc tcgcatcgaa cgtgatgaag cgcgcgatgt ttggatgctc gggcaacgcg
      481 agcagcgcgc cggcctcctc gcggaacagg cgcacgaact ctcgctcgct gagctcacgc
      541 gcggcgtcgc cagagtagtc ggggaccttg accgcgacga gctcacggtc ggcgcgcgtg
      601 cgctcgtcgg cgcgcaccgc gagcaacacc gagccgcccg caccgcgtcc gatcggacgc
      661 acgacgtaat agccgccgag catgcgcgag agcggtaccc agccaggcag cgtgacatcc
      721 gcggttgccg tggtcgattc gctgcgatcg attggtcggc gcgcgagcca gaccagcacg
      781 cgttcgatcg cggccgcgag cgccggcggc agcgcggctc tgctctcgtc gatcgacgcg
      841 gcgatcgcct cgtcgagcgc agcgctgcgc gcatcgcgct ggtcggtcgc aaggacggcc
      901 tcgattccac gcacggcagt ttcgagcgag cccgcgtgat cctctgcgtc gatgccgagg
      961 cgccgccgtg ccccgaggct gcgccgcgcg agtgcaccga gctcggtcgc gatcgcatcg
     1021 agcgtgccgg ctgacaccgc ctgctggctc gataccgacg tgaggcgggt gagctggttg
     1081 ccgagccgcg cgagtgcgag ccgcaccgat tcgacacgtg gcgattgttc cggcggcaat
     1141 gcgtcggtga gctcgccgag ccgctccaca acgactcgca gcgcttccgc gtggtcggga
     1201 tcggcggctt cccaggtcgc ttgctggagc gccgcgtacg cctgaaacga ttgctcgacc
     1261 tccggcacca tcgacgcgtc gcgcacgatc gcgaagtcgt cgtcgggaaa cgcggtcgtc
     1321 catgcgaaca acagatccgt gagctccgca tggccgtcgc gcaatagcgc gtcgccggcg
     1381 cgcgtcatcg ctgcccacac cgcgcggcgc agtggtgatc gatcgacttc cgcgcgcgcc
     1441 atcagctgac gtacggcggc gagacgcggc tcgagatctg cgtcgcccgt gacgcgcacg
     1501 ccgtcgagca agcgcacgag cgcgcggagc cgcgcgatgc gaagcccgcc gtgtgcgact
     1561 gcacccgatt cgggcgcgcg ttcgtgagca aggagcgcgg cttcgatcgc agcgagggca
     1621 cgcgcgaact gctggcgtgc cggatcggca tcgccggcga gtgcgagcac cgcgggcaac
     1681 gcgttgtcgg caagtagctc gcgatcgagc tcgcgcagaa ggcgcatcga atgacggcga
     1741 tcgattggat cgtcatcgct ggcgcccttc agccattccg cagcgtcgct gatctcgttc
     1801 acggcagtgc gcgccgcgcg aagcgcgcct tgcgtgtcgc catcgtcgag cgccgtgcgt
     1861 gcttcgccga tcggcacgcc gattccttcc gtgaccacga tgccgccgag ctccgcctcg
     1921 agcgcgtgca gcagagccca ccgaccgtcg tctttcggat cctggtccgc cttcatctgt
     1981 gcacgcgtcc agtcgagcgc ggcggcttcg gcctccggca acaacgtgcc cgcttcctcg
     2041 cgacgcagtt cggcgagcgc ttcggcgcac tcgagcggct gacgagcgac gagcttcgtc
     2101 gcgagctcgt cggcctctgc cggcgatgcg atcgcgagcc cagcgagccc gagaatcgcg
     2161 ccgcgcccga cacccggctc tttcgccgcg cgcgcgatca gcatgtcgtg ccagcggcgc
     2221 gctgcgccac cacgctcgag cgccgcggcg gccgacgcgg caccacggcg gacctcggtg
     2281 ttcgacgcgg ccgggcgcag ctcgcgatcg atctcacccg cgagatccgg atcgacgtgc
     2341 gcgaggatcc cgcgcgcgat gctcgcgaag cgcgacacca gtgcctcgcg atcaccgagc
     2401 agccgcgcaa gcgccgctcg cacacccggt cgcgcgagca cgcgcacacc accgcgatcg
     2461 ccggcgtcgt ggcggcgcac ggcttcacgt gcgccatgcg cgaggatgcg tgcagcgagc
     2521 cggcgcccgg gtagcgatcc ggtcgccggc tcctcgaccc agctgtgtgc gagctcgggt
     2581 tgcgcgagca aaccgatcgc gagctcgccg atcatgccgg gcgcggtgaa cggcgccgcg
     2641 agcacgacgc taaggcgcgc acgcagatgg tcggcagcga cgatcttcgg cgacgtacgc
     2701 gcgagggcga tcaacaaccg cacgaacgtc gcgcgatcgg cagcgcgcag cgacgtgagc
     2761 acgcgacggc cgagctcgcg acgctcggcg cctggtggca gcgcttgtgc gagctcgaac
     2821 agcgcgatcg cagcagacgc cggcgacaac caatcgagcg aggtcagcag gccatcgccg
     2881 aacgccgcgc gcaccgcgga gagcagctga tcggcggaga tgccttcgag cggcgctggc
     2941 tcacgctccg agatcgcggc gagtgccgcg agaaactgtc gggtggcctg acggcgctcg
     3001 tctgcggttg ccagcgtgtg gagcgcggca aaacccgcca ggtaggacct gcctcccgac
     3061 attcagcaac atcttacgcc gctcacggct ggggcgtcag agcctgtggg gtaagcgtct
     3121 ccttatagaa ggtcacgccg tcggggccga cgcccgagaa cgtcagctgc tcaccgtcga
     3181 cgtcgatcaa cgcgaagttg tactgcatgc tgcgaaacag gacgcccggc gcgaatacgt
     3241 gcggcgtgcc gatgccacgg cagaccgggc cgacctggaa ttcgcgaatt ccgtgcgcat
     3301 gcttgtgcgc ggcgaaccag tgctggtccg cgctgacgaa caagatgccg ggaatgccga
     3361 ccagggcgtc gaagatgagg ctgcgctcgt acgtgtacgc tgcccagtga tcgttgccgt
     3421 cggcgaaatc gagcggcacg ctcgtgaaca cgagcttgaa cgtcgcggtc gagctctcga
     3481 gcgtatcgat gagccacgcg cgttgcgtgt cgccgagcat cgtcttgttc gcatcgtcgg
     3541 gcgctgcgtt cgcgctacgg aagcgacggc agtccagcat gatgcagtcg acgtgcgcgc
     3601 cccagcgaaa cgcgcgatag cgaatctcgc cagcggcgcc gcgcaccgga aagaactcgt
     3661 cccacacctg catcgccgcg gcgtagcgac tcgcttcggc aacgcgatac atgccgtcga
     3721 agtcgttgcg aaactcgtgg tcgtcgtaga tcgcgcgcac cgccatcgct tgcagccacg
     3781 cgcgctgccg cggcgcggtc accgtctgcg cgtagcgatt gcgatacgcg tcgacggtca
     3841 cggcgacatc cggcccgttg tcggcgtatg gaaaatcgcc gatcgagaca aacacctccg
     3901 ggctcgccgc cgcgcaatgc gcgagcaatt cgctgtcgaa ttcgaggttc ggatcgacat
     3961 cggccgacac cgcgatgcga accggccggg gatcgtcagc gagtggcgcg gtgcgaacat
     4021 gatgcgggcc gagctccaca ccggtgtcga acacgaggtg aacttgataa ttcgtcgacg
     4081 ctgcgagccc ggttacgtcg aatgaaccga tctcggaaat ctccatcacc gtcgacatca
     4141 cgatggcatc acctgactgc acttcgagcg caacggtcct cgcctccgac gaccagacca
     4201 cgacgatgaa ggagctttcg tcgggttcga cgacgaacgc cgcgtgacta ttgcccgcgt
     4261 cgcgtgggcg ctcgttgtcg ccacagccaa cgatgaggct cgccgacccg atgccagcga
     4321 ttcgcagcac gtcacgtcgc gtgaggccca cccgattcat ggtgacaggt cagcgtagcc
     4381 gtcgtttaat ccgaccgcca tgatgagggc atcgctcgtt cttgcgttct gcctggccgc
     4441 ctgtgccttc gagcatggga cggatccgac gacgggcggc gccgacccgg gctctgggtc
     4501 cggctccggc gatccagacc aggacaccga cggcgacggc aacaccgacc ttaccgataa
     4561 ctgctccggc gtcagcaatc ccgatcagca cgatcacgat gacgacggcc gcggcgatgc
     4621 gtgcgacgtg tgcccgcacc tcgtcgacac cggtaaggac acggatggcg atggcgttgg
     4681 cgacgcgtgt gatccgcggc cgaccgagcc tggtgatcgc atcgcgttct tcgagggctt
     4741 ctacgacgcg ccggcgtgga catcggtgat cggcgccaac acgtggcaga tggaatccgg
     4801 cacgctgcgc cagcacggcc tcgacgccgc gtatcagctc gtgcgcgacg acaatccaga
     4861 tctcggatcg gtgctcgtcg atgcgcgcgt gaagatcaac gcgatctcga acaacggctc
     4921 gatgcgccgc tcgaccggca tcgtgatgtc ctatcgcgac gcgaaccact acatcttctg
     4981 cggtctcgcc gctccgcaat catcgcagca gggtgcggag gtcaacgccg gccaggtgtc
     5041 gaccgacttc tggggcagcg cacagtacaa ctatgcgcct ggggtgtttc cggcacagat
     5101 ggcgggcgac tggctcacga tgcaggcgcg cacgacgaag acggattggg gcacgacgca
     5161 gatcgactgc gtgactcacc gagccggcac caccggcacg gcgacgtacg acggtgatgc
     5221 cgagatcgac ggtgacatcg gcatccgcac gaacggcgcg gatgcgagct tcgactacgt
     5281 gtttgtcgtg gcctcgcctg ccgcaacgta gagcacgcga cgtcggcacg ccgcatgctc
     5341 gttgttacga gcatggcgac ccacaaagat tccagagctc gtgacgcgcg gcgggcgatg
     5401 cgggaagcca gtcgtctgcc gcagccgtcc attgtcgaca tcgatcccga ggagccgact
     5461 cagctcctgg atgatgttgg tcagtacagc gtgatcgatc tcgatctcgg ggtcgaagcg
     5521 cagccgcacg ccgaagccga cgcgctgatc gatgctgcga tcgacgaggc cgaaggcggt
     5581 gaagcggaag aggaggacga gctcgaggcg cggtcgcgcg acgaggccga agttccgatg
     5641 aaggacaaag acaccggcga tctgtacggc gtccatcagc cgccggcgac cgatcgacag
     5701 ctcgacaaga ccgccaacca ggaggtcttc ctcgacgcgg accaaggtga gaactggctc
     5761 gaaacactcg gccacaagtc ggtcgagtac ggaccggaaa ccgagcacca ggtcgacgtc
     5821 atcgacgaca gcgacgagca cctcgatcac cgcggtcacc acgcgagcga atccggcgac
     5881 cggccggtcg ccgacaaggg ctccggaggg gatggcgggc tctgacgtaa aggtcggtag
     5941 tgcgccagct ggacctgccg ggtacaccgt gtgaaggtcc gcgcgtgaag atcgagcgtc
     6001 tcgacatcgc aggcctgacg acgcgcgtcg tcggcaacgg caaacagacg gtcgtgctgc
     6061 tgcacggttt tggcgcgccg ggcgacgatc tcgtcgcgct cgcgcagtac atcgacgcgc
     6121 ctgcgcggtt cgtgtttccc gaagcgccgc tcgagctcgg cggcttgtac ggcgattcgc
     6181 gtgcgtggtg gcagctcgac ctcatgcggc tcgagcagga gctgagcagc ggtaagccgc
     6241 gcgatcgtcg tggcgaagtt cccgagggcc tgagcgatgc gcgcgaccac atggtgcgct
     6301 tcctcgagga gctcgaggag cgtttcgcga tcgagaactc acagctcgtg ctcggcggct
     6361 tttcgcaggg cgcgatgctc gcgctcgatg tcgcccttca tcgcgaggtc gcgccggcgg
     6421 gtttgatcct gatgtcgggc acgctgctcg cggagtcgat ctggcaaccg ctgatgtcga
     6481 agctgtccgg tgtgccggtg ttccagtcgc acggcatgca cgatcaactg ttgccgttct
     6541 cgatcgctcg ccagctgcgc gatcagctca aagccgcggg cgctcgcgtc gactggtgcg
     6601 agttccacgg cggtcacgaa atcccgccga gcgtgctcga tggcgcgacg aagctactgc
     6661 tgactactgg cagtatgtcg cggtgagcgc gaacgagtcg aagaagaagt tcgtgacgaa
     6721 cgagtcgtcg ttcgtggacg tcatgcgcag ccgcacggtc tgtcccgaca ggttctgcgg
     6781 gaacgtgaaa ttgatcgcgg tccacgtcgc gccggtggtc gtcaggttgc tgagcgagag
     6841 cacggtctgg atcggcgtgc cgttcgtctg cgtgagcccg agctgaccgc tgtcgtagac
     6901 cgacgtcgtc gtggtctctt gcgtgccgac cacgtagtag ccggtgagcg tcagctgcgt
     6961 cgttcccatc ggcaccgtga cgtcttgata gagctggtcg gtcacgcttt gaccgagcgg
     7021 tgcttcgaag ccgcccatcc atgccttgta cggcgcggta tgcgcggcga ggccctgatc
     7081 agtgacgacc ggatacgcgt tgtcgatgtt cacgagcgtc cagccggtgc cgaccggcgt
     7141 gagatcgagg acggggttcg tgagcagctg gcgcgtcatc acggtgcact gatacgcgtc
     7201 gggcatctgc accgatgcat ccggcggcgg tggcggcgca tccggcggca cgaacgcatc
     7261 gatgggctgc gcatcgaccg ggggcttcgc gtcggcgagt gcggcatcga cctggccttc
     7321 gatctgacca tcgacgcgcg gatcgatgat gtcgccgccg tccgtgcccg agacgttcga
     7381 gcgaccggac gcgcacccga caaccaccag cagcgcagcc aaatgcttca tttgtttatg
     7441 ggccgtggga tatcccggtt gcgaatcgct ttcaacgaac atttcgcaac cggcatgagg
     7501 tccccagacc cgaggacggc gaatcgcaga cctcgggtcc tgggctatgc cctcgatcac
     7561 actgcggtac ctgccattgc ccggcgtgcc gccgcggcgg ccggccgcca gcccccgcgc
     7621 aggacgcgga gccagaacag cgacgcaccg aggacgatct cgacggccag gcccacccag
     7681 ccgccgacca cgccgagccc gagatggaca ccgagcagcc acgtgagcgg cggtgtggtg
     7741 agccacgagg tgatgatccc gacgaccgcc gcgaagcgga cgtcgctcgc accacgcagc
     7801 acgccacgcg cgatcacgtt cgccgcatcg gcgacgagga acgcgagccc gacgtagacg
     7861 agcagcgtcg cccgttcggc gagcgcggag tcacccgacg ccatcgcggt cgctatcgtg
     7921 ccgccgagga tcgcgtagcc cacgagcacc acggtcgagt agccggcacc gatcgcgagc
     7981 gcacgtctgg ccacgcgcgg cacgagcacg tccttgcccg caccgaccgc ttgcccgacg
     8041 agcactgccg ccgcttcggc tagcgcatgt gccggcagga acgacacgtt caccaggtgg
     8101 agcaccatct gatgagccgc ggcgtcgacc gcggacatgc gcgcgacgag cacggtgagg
     8161 atcaagaacg agccgacctc catgatgaac tgcaggccgt tcggcacgcc ttgcgcccac
     8221 acgtcacgcg ccgctttgcg cgaccagccg agcttcttca gctgtgcgcg catcggccac
     8281 gcgagcaccg cgagctcggt cgcgttaccg gcgatcgtcg catacgcggc gccgcgcaca
     8341 ccccagtcga ggacgaggat gagcacgatg tcgagcgcga tgttgaccgc gttaccggcg
     8401 agcgatgctc gcatcggcgc gcgcgtatca ccgatcccgt agcgcgattc gcgcagcgcc
     8461 gcatagacga gcacgagcgg cgcaccgagc gaacggatgc cgagatattg cgcggcgaaa
     8521 tgacccgcgc gcggtgacgc cgacagatgc gcgagcagcg gcgcgaccag ctgaccggtg
     8581 acggtcgcca gcacgcccag cacgatggcg atgcagagcg cagcaccgac gagctcgggg
     8641 atcttgtcga gccgatttgc accgacggct tgcgagacga gcgtcttggc accgcgcaga
     8701 aggccgatgc cgaagcacac gagcgtgaag ccgacgacgc cggcaagacc gacgccggcg
     8761 agctcgtccg caccgacgtg cgcgacgaat gccgtcgacg cgagcgtcat cgcggaaaac
     8821 gaaaccatgg agaccgtgat cggccaggcg aggcggagca gcgctcggcc gggcctgtcg
     8881 gaccaggtca aagaaacatc tgaggtggtt actaacgcag acatgacgag cctctaacga
     8941 gacaaggacg atcgaaccct cgcggattcg acaagagccc caacgtttcg ccgaaaactt
     9001 gaagggctcc cttttccagg agcccgcggc ggtgaggtta gctctcgatc agcgcatcga
     9061 gcctctgcca ccgcgggcgg tgccgcgatc gacgatgtaa agcttcgtcg tcggctggca
     9121 cattgggcgg ttgcagttgc tcatcggctg ttctccgagt ccttgttgtc actctcgcaa
     9181 atcgttgcgc gatccgtcaa gaacattgac cggcacacgc gcgcgctacg tgatcgagat
     9241 cagcggtgcg acgcgggtgc gaccagcgaa acaccgtacg tagaacgcgg attcgcgcag
     9301 gggccggtat ccaggcatta cgcgagaaca cgcaggccgg gaatgagtca tgggttcacg
     9361 cggttgacag gccgcccacc ccaaggatta cctccactcc tcgcgcctat gtccgaagac
     9421 actgccaagc tgcaaagcca attcaccacg ctcaaatccg aagtcggcaa ggttctggtc
     9481 ggccaggacg acatgctgca cagactgttg ctcggcttgc ttgccggcgg acatgtgctg
     9541 ctcgaaggcg ttcccggcct ggcgaagacg ctcgtgattc gcacgctcgc cgatgccctg
     9601 gacggcacgt tctcgcgcat ccagttcacg cccgacatgc tgcccggcga cgtcgtcggt
     9661 acgcagatct acaacccgcg tgaaggtacg tacaccgtca agaagggacc ggtgttcggc
     9721 aacttcattc tcgcggacga gatcaaccgt gcccctgcga aggtgcaatc cgcgctgctc
     9781 gaggcgatgc aggagcgtca ggtgacgctc ggcgatcaga cgttcaagct cgccgatccg
     9841 ttcctcgtgc tcgcgacgca gaacccgatc gaacaggagg gcacgtatcc gctgcccgaa
     9901 gcgcagctcg atcgcttctt gctcaaggtg aaggtcggct atccgacgaa ggacgacgag
     9961 gtgaagatcc tcgatcgcat ggccggcgcc ggtgccgagc cgcatgcgag caaggtgatg
    10021 acgtgcgacg agatcctcgc cgcgcgcgac aaggtcaagc aggtgttcgt cgacgacaag
    10081 ctgaagcgct acacggtcga catcgtcgcc gcgacgcgcg atccgaaggc tgcggggctc
    10141 ggcgcgctcg caccgctgat cgacaatggc gcgtcaccgc gcgcgtcgat cgcgctgatc
    10201 aaggtcgcaa aggcgcacgc gctgctatcg ggccgcgatt acatctcgcc gcacgacatc
    10261 aagacgatcg cgcacgacgt gcttcggcat cgcctcttgg tcagctacga ggccgaggcg
    10321 cagggcaaga cgaccgatca cgtcgtcgca caaattctcg agaacatccc cgttccctga
    10381 ggacgcatgc ttcccgccga gatcgcgcaa gccgtcaagc gcatccagtt cgtcaccggc
    10441 cggcaggttg ccgacgtgat ggcgggcgca tacctgagcg tcttcaaagg acgcggcatg
    10501 gaattcgacg aggtgcgtcc gtacgtgccc ggcgacgacg tgcggtcgat cgactggaac
    10561 gtcaccgcgc gcacgggcga gccgcacgtc aagcgcttcg tcgaggagcg cgagctcacc
    10621 gtgatgttgc tcgtcgacgt gtcggcgtcg caggatttcg gctcgggccg gcgtagtaag
    10681 ctggaggcgg cggtcgagct ctccgcactg ctcgcgctgt cggccgtcga gaacggcgac
    10741 aaggtcggca tgctgctgtt ccacggcggc gcggacctct acatcccgcc acgcaaaggg
    10801 ggcaagcacg cgcttcgcgt cgtgcgcgaa gtgctcgcgc gcggcaatga cattgcgacc
    10861 gggccggcaa agcgcgagct gttgccacgc ttcgtgcgcc gctggttcaa gctgctgcaa
    10921 gacaagacgc gcaccgagcc acgtcgatcg acgagcatcg cggctgcgct ggagttctgc
    10981 cgccaggtgc tgccacgccg ggcggtgctg ttcttgatct cggacttcct cgacgatggc
    11041 tacatccagg tgctgcgtca cgcgaaccgc aagcacgacg tcgtcgcggc gctgatcacc
    11101 gatccgcgcg aggccgatat gccgcccgcg ggtctcgtca cgctcgagga tgccgagtcc
    11161 ggcaagctgc gcctcgtcga tacgcgcagc aaagcatttc gcgatgacct cgcgacgcgc
    11221 gcggcaacgc ggcagaagga actgcacgat cagctgcgcg cgacgggcat ggatctcgtc
    11281 acgttcgatg cgtcggggtc gatggtcgat ccgctgctca agttcttccg cgagcgcgag
    11341 cggaggctgc gccgatgaag cgcttgctgg tcatcggagc gctggcggcg tgcggcggca
    11401 cgagcatgct cgatcagtcg cagcccgcga agagcgagcc gacgacggac gcgctcaaga
    11461 agacgaccga gaacggcccg gtgaaggcaa ccgtgaccgt gtggccgccg aagccgacgc
    11521 tcggcgagcc gatctatttc cgcctcgacg tcgacgcgcc cgctgggatc agcgtcgatg
    11581 cgccgttcca gg
//
LOCUS       DASUFX010000799         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_547168,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000799 DASUFX010000000
VERSION     DASUFX010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547168"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFV99_32300"
     CDS             complement(<1..471)
                     /locus_tag="VFV99_32300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064118.1"
                     /translation="MNRPRHTGLQWKLTVAVVAIVIVPLVASAILIDQIGKVAANFGA
                     TESKERASMIEQVSRVYRDLVSTTKSLQVEVAQRIASRPDIANPIPEITLEKILDEEA
                     NLRAIAVLRPDGSVVDEASKPVEPDETKWRDAVVDHQLPNGSSLRLTFRVIDTSN"
     gene            complement(468..1019)
                     /locus_tag="VFV99_32305"
     CDS             complement(468..1019)
                     /locus_tag="VFV99_32305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064119.1"
                     /translation="MRFVERGDQLTTTGNIAKLFDQAAYEQLSSGFASTIQIGTAVYP
                     KGSKEPIAVRVELRTVVYDLWDEQYVIRIEGQAGKRTRKVKYRAEALKIITAIDDFPV
                     ANLADIPYEDVFYLNVTAQLNPVSKETLAEVRRWLSQGTGGGLDRGGVFFGSFVSVFV
                     NPKIADADRVLRIRSQPFYRPKP"
     gene            complement(989..2596)
                     /locus_tag="VFV99_32310"
     CDS             complement(989..2596)
                     /locus_tag="VFV99_32310"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5064120.1"
                     /translation="MRGFAALLLALLATSAHAEDAIPAAPVPGPAPTTAPAPAPAADE
                     ETKLNPDDEFGPVLLIERIDITGNTNTQDEIIRRALPIAPGDILHASDKRLRNARFKV
                     LALGYFRDVTLVMGKGSQRGNVIIEVNVVERGTFVLNRLWFGTTRASPYWFGTDVGDR
                     NLLGLGISVGGGFIYAANGSIDGTRNQWAGEIRLGDGSLLGSRWGANGSLTFVHGSDF
                     YRISGDSDDINTDHFRGFPYSRFGGRFGATYDITALSRLSLAARIEGISADVPTTPTQ
                     LLPDGRTANVDLHLEPGDSRVITAGLGIDRDTRSDPILPHSGGRITAAVEVGTSALGS
                     DYDFATVFARYEHWWPLRDERHTIGIRLAGGVVIGNAPRFDRIYIADVNRMLTPRALG
                     LVLSNAAALDILSTSADKPAYGDLGGSAGVEYAARMFRGSGRRRVYGGDFFVGAGVWG
                     LAESQDVQLRDASVWNSLPIDLYIDAGVRIDTDFGIFELTGANALGRLRGERSRSRRS
                     CLSPCRHSRTTTSPKRSACVSSSAAIS"
BASE COUNT          462 a          876 c          907 g          482 t
ORIGIN      
        1 gttcgacgtg tcgatcacgc ggaacgtgag gcgcaagctg ctgccgttcg gcagctggtg
       61 atcgacgaca gcgtcgcgcc acttcgtctc gtcgggttcg accggcttgc tcgcctcgtc
      121 gacaacggag ccatcggggc gcagcaccgc gatcgcacgc aggttcgctt cctcgtcgag
      181 gatcttctcg agcgtgatct cgggaatcgg gttcgcgatg tccggccgcg aggcgatgcg
      241 ttgcgcgact tcgacctgca gcgacttcgt cgtcgatacc aggtcgcgat agacgcggct
      301 gacctgctcg atcatcgacg cgcgttcctt cgactcggtc gcaccgaagt tcgcagcgac
      361 cttgccgatc tgatcgatga ggattgccga ggccacgagt ggcacgatca cgatcgcgac
      421 gaccgctact gtcagcttcc actgcaggcc tgtgtgacgc ggacgattca tggtttgggc
      481 ctgtagaacg gttgcgagcg gatgcgcagc acgcgatccg cgtcggcgat cttggggttg
      541 acgaacacgg acacgaagct gccgaagaac acgcctccgc gatcgaggcc gccgcccgtg
      601 ccttgcgaca gccagcgtcg cacttcggcg agcgtttcct tcgacactgg attcagctgc
      661 gccgtgacgt tcagatagaa gacgtcctcg taggggatgt ccgcgaggtt cgcgaccggg
      721 aagtcgtcga tcgcggtgat gatcttcagc gcctcggcgc ggtacttcac cttgcgcgtg
      781 cgcttgccgg cctgaccctc gatgcggatg acgtattgct cgtcccagag gtcgtagaca
      841 acggtgcgga gctcgacccg cacagcgatc ggctccttcg agcccttcgg atagaccgcg
      901 gtgccgatct ggatcgtcga cgcgaatccc gagctgagct gctcgtaggc ggcctggtcg
      961 aacagcttgg cgatgttgcc ggtcgtggtc agctgatcgc cgcgctcgac gaaacgcatg
     1021 cgctgcgctt cgggcttgtc gtcgtccgcg aatgccggca aggcgacaag cacgagcgcc
     1081 gtgatcgcga acgctctcct cgcaaccgtc ccaacgcgtt cgcaccggtc agctcgaaga
     1141 tgccgaagtc ggtgtcgatg cgcacgccag catcgatata cagatcgatc gggagtgaat
     1201 tccagaccga ggcgtcacgg agctgcacat cttggctctc ggcgagcccc cacaccccgg
     1261 caccgacgaa gaaatcgcca ccgtagaccc gccgcctgcc cgagccacgg aacatccggg
     1321 ccgcatattc gacgcctgcc gagccgccga gatcgccgta cgccggtttg tcggcgctcg
     1381 tcgagaggat gtcgagcgca gccgcgttcg acagcacgag gccgagtgcg cgtggcgtga
     1441 gcatgcgatt gacgtcggcg atgtagatgc gatcgaagcg tggcgcgtta ccgatcacga
     1501 caccgccggc gagtcggatc ccgatcgtgt ggcgctcgtc gcgcagcggc caccaatgct
     1561 cgtagcgcgc gaacacggtc gcgaagtcgt aatcgcttcc gagtgcagag gtgccgacct
     1621 cgacggctgc ggtgatacga ccacccgaat gcggcaggat cggatcggag cgcgtatcgc
     1681 ggtcgatacc gagcccggcg gtgatgacgc ggctgtcacc cggttcgaga tgcagatcga
     1741 cgttcgcggt ccggccatca ggcaagagct gcgtgggcgt cgtcgggacg tcggcggaga
     1801 tcccctcgat gcgcgccgcg agcgagagcc gcgagagcgc ggtgatgtcg taggttgcac
     1861 cgaagcgccc accgaaccgg ctatacggaa agccgcggaa gtgatccgtg ttgatgtcgt
     1921 cggaatcgcc gctgatccgg tagaaatcgc tgccgtgcac gaacgtgagc gagccgttcg
     1981 cgccccatcg cgagccgagc agcgagccgt cgccgaggcg aatctcgccc gcccattgat
     2041 tgcgcgtacc gtcgatcgag ccgttcgcgg cgtagatgaa gccaccgccg acactgatgc
     2101 ccagcccgag gagattgcga tcaccgacgt cggtgccgaa ccagtacggt gatgcgcgcg
     2161 tcgtcccgaa ccacaagcgg ttgagcacga acgtgccgcg ctcgacaacg ttgacctcga
     2221 tgatgacgtt gccgcgttga ctgcctttgc ccatcacgag cgtgacgtcg cggaagtagc
     2281 cgagcgcgag caccttgaac cgcgcgttgc gcagtcgctt gtcgctcgca tgcaggatgt
     2341 cgcccggcgc gatcggcaac gcacgacgaa tgatctcgtc ctgcgtgttc gtattgccgg
     2401 tgatgtcgat cctctcgatg agcagcacgg ggccgaactc gtcgtcgggg ttgagcttcg
     2461 tctcttcgtc ggccgccggt gctggcgccg gcgccgtcgt cggtgccggt cccggtaccg
     2521 gtgccgcggg aatggcatcc tctgcgtgcg cgctcgtcgc gagcagcgcg aggagcaaag
     2581 ccgcgaaacc ccgcatctcc cgcgagcgta gcgcaccgat cccagcgggt gctaccttgc
     2641 ggctgtgcga ggagccacgg tttggttcgc tatcctcgtc ggaatcgcgg cgtgcaacga
     2701 cgtgcgcgac tttgccggcg attggca
//
LOCUS       DASUFX010000800         5881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_685433,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000800 DASUFX010000000
VERSION     DASUFX010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685433"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="VFV99_32315"
     CDS             <1..2217
                     /locus_tag="VFV99_32315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064121.1"
                     /translation="EALNYDAENERGVFLDNYTYLPASVKGDCTPGHTWGLFLLDEKV
                     LMVGSYDSSNGRLTNCWLAPFQLMADTSQEVTGFIGRRADEPPAPITMRQVHIIEATE
                     ETLINLLIYGSGTPGYNHGTYDVLGFGLGLGMPGELYGFDFERTVGNLPRANAPAAII
                     LDEPKSLGDIIRDDLRIRWSFFRWKDQHFEMAQWKTPVAELAIGTLTEANKAAPAGMD
                     DDHRVASRETDEFQKGTAKIDYSRDYAVSRNEQYQRSVSVEDQTAVDDSGGGIGSVAM
                     KMRNTYAQMQNTGAAIEGLLPDFISMFAVMARPLSQIARSIDPRFYEGPSVGDVFLVN
                     DKFARDPITGTRGIGSRPAMVTRHWYSWGGPSPTSDEPRPADGGVELMFLDLQRGSKY
                     SPAAEVDETANSGGFTAGYNSGTATLRCKPHAYSHVITITTKRGFIEIAEGLDASNFD
                     VNEIVDIVQIDPDDPATAITWSNRTITVRSGNDITINPALSSPSFDSTKLYRITSAKY
                     SQAAATQKDDVYQGDDADEMIEDVEFPYQFSATEEAYDYNGNTGTEKGELVAAAAYGD
                     GRPWDVGHDRALINLENAFIDGLSAHQAPFLWSEFADDVGSAADWYTLWMGPFFFGTE
                     HLTTAVYRLLTLAPWMRSSSGGTTGYIRATLSRVVPIGSPGTSGTVTDKGRFTSAFAQ
                     AEWSTSSATWGEQPAQTLELSVKDLFWGFAWLVIEKKGTGQLRGFSRFVEGARRIR"
     gene            2217..3359
                     /locus_tag="VFV99_32320"
     CDS             2217..3359
                     /locus_tag="VFV99_32320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064122.1"
                     /translation="MPQLKFTGDRLPIGSVGSRQVVANPTSKAADPPIQYDRFARNGK
                     HPNVALIQQMQGAVNAAVMYRSKQVFSIAGRLNTAAPVVSAADRPRWRFAFHTSPYTH
                     ALMAVVSMLPPDVDISPPGYANTSGRLDIANGAGSIVATQSFYYGLHPVPRFSGLGGY
                     VPDWKHIRWFVKYVDGVAADTDYFGTFYDVNYGRLQSACVFELPSMTEFTNGYLAENI
                     SGQSPILDVYREKQATIMRNLWRRAGAHLINWTVDDQGAPLQRTANTTQVNVIDLSTT
                     FNASDPAFTLVMDGKNRRTQSAGIPCVMKAYGVMSAGTNGSVYLKNGVGTIVAQIVSA
                     WGTTPSWQTSAVFNLPATTDRYFLHVAGPASGTFSLGAISVYPYTT"
     gene            complement(3360..3602)
                     /locus_tag="VFV99_32325"
     CDS             complement(3360..3602)
                     /locus_tag="VFV99_32325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064123.1"
                     /translation="MKHLALAFLVLAACTDDQPDPTPHDIGKTVVEMCGQPPLAPGWQ
                     VQSNGGVVTMSTDEYNAIVKYRDDSAAWRECAIAIP"
     gene            3761..4093
                     /locus_tag="VFV99_32330"
     CDS             3761..4093
                     /locus_tag="VFV99_32330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064124.1"
                     /translation="MTVARLALLLALLGGCSTYDAFAGRIDSPGSVYRCDDTPNGPLE
                     YCYFEDAAAELSAATGLRCYESGLFDRWATAFGCWYHCDDPSTLENEGDGKGCNAHLG
                     GCFCEAMP"
     gene            4090..4617
                     /locus_tag="VFV99_32335"
     CDS             4090..4617
                     /locus_tag="VFV99_32335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064125.1"
                     /translation="MTDEKKPRATSQPAADSFAADDASQFTPVTDVLELLDAEIVLTE
                     RERRMFFYLWKHSANQEMRGRKRSDSQDTSLLAKRLDEIDDKHTRLDVDLRGDGNREH
                     GRVATLERFHARIVKWALGSLVAIAGAAISFGIWMGSVRTDIDNLKQRAQPSQRWRSF
                     LPDSSASPDSKEPTP"
     gene            4614..5135
                     /locus_tag="VFV99_32340"
     CDS             4614..5135
                     /locus_tag="VFV99_32340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064126.1"
                     /translation="MITRLSSFASSFAGAVVLMSALVFGAMFTAYSCTRAAYAQEGSG
                     SAMVVDAGSASAPAPDTAQPAPASTLDELSALKAKYEALKTAQPDAKMLAWAALIAAA
                     LKLMLSLFNRYVFKETKTWTKWIALGAAVPIALLSYYALGNGLFASLVYAGAGPGAII
                     VHELLQKKAPSSS"
     gene            5132..5365
                     /locus_tag="VFV99_32345"
     CDS             5132..5365
                     /locus_tag="VFV99_32345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064127.1"
                     /translation="MSDRDEEQRRLIVVYGDPFACDSPGHRRLKAEETVRRALGTRQK
                     QGRRLSVELEMPVWVVVLCFVMLVIGLVYGALR"
     gene            5362..5613
                     /locus_tag="VFV99_32350"
     CDS             5362..5613
                     /locus_tag="VFV99_32350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064128.1"
                     /translation="MTAADYKRSLYPVEGLLAGEWTDETATGRPAVCCARCSGIYDIP
                     ETHRIDPAGFVRPELKCGAGCSEVGYGVRLLNYADEVLR"
BASE COUNT         1042 a         1918 c         1952 g          969 t
ORIGIN      
        1 gaggcgctca attacgacgc cgagaacgag cgcggcgtgt tcctcgacaa ctacacgtac
       61 ctgcccgcat cggtcaaagg cgattgcacg cccgggcaca cctggggcct gttccttctc
      121 gacgagaagg ttctgatggt cggcagctac gactcaagca acggccgctt gacgaattgc
      181 tggcttgctc cgtttcagct gatggccgac acaagccagg aggttaccgg cttcatcggt
      241 cgccgcgccg acgaaccacc ggcgccgatc acgatgcgcc aggtccacat catcgaggcg
      301 accgaagaga cgctgatcaa cctgctgatc tacggcagcg gcacgcccgg ctataaccac
      361 ggcacctatg acgtactcgg gtttggtctc gggctcggca tgcccggtga gctgtacggc
      421 ttcgacttcg agcgcaccgt cggcaacctg ccgcgcgcga atgcaccggc cgcgatcatt
      481 ctcgacgagc ccaagagcct gggcgacatc atccgcgacg atcttcggat ccgctggtcg
      541 ttcttccgat ggaaggatca gcacttcgaa atggcgcagt ggaaaacgcc ggtcgccgag
      601 ctcgcgattg gcacgctgac cgaggcgaac aaagccgcgc cggcgggcat ggacgacgat
      661 caccgcgtcg catcgcgcga gaccgacgag tttcagaagg gcacggcgaa gatcgactac
      721 agccgcgact atgcggttag ccggaacgag cagtaccagc gcagcgtgtc cgtcgaggat
      781 cagaccgctg tcgacgattc gggcggcggc atcggatcgg tcgcgatgaa gatgcgcaac
      841 acgtatgcgc aaatgcagaa cacgggcgcg gcgatcgagg gcttgctacc cgacttcatc
      901 tcgatgttcg ctgtgatggc gcgaccgctc agccagatcg cgcgctcgat cgatccgcgc
      961 ttctacgagg ggccgtcggt cggcgacgtg ttcctcgtga atgacaagtt cgcgcgcgat
     1021 ccaatcaccg gcacgcgcgg catcggcagc cgcccggcga tggtcacgcg acactggtac
     1081 tcgtggggcg ggccgtcgcc aacgagcgac gagccgcgac cggccgacgg cggcgtcgaa
     1141 ctgatgttcc tcgacttgca acgcggatcg aagtactcgc ccgcggccga ggtcgacgag
     1201 accgcgaaca gcggtggatt caccgcgggc tacaatagcg gcacggcgac gctgcgctgc
     1261 aagccgcacg catacagcca cgtcatcacg atcaccacga agcgcgggtt catcgagatc
     1321 gccgagggcc tcgacgcgtc caacttcgac gtgaacgaga tcgtcgacat cgtgcagatc
     1381 gatcccgacg atccggcgac cgcgatcacg tggtcgaacc gaacgatcac cgtgcgcagc
     1441 ggaaacgaca tcacgatcaa tccggcgctt tcgtcgccgt cgttcgactc gacgaagctc
     1501 taccgcatca cgagcgccaa gtactcgcag gcggcggcga cgcagaagga cgacgtatac
     1561 cagggcgacg acgccgacga gatgatcgag gacgtcgagt tcccgtatca gttctcggcg
     1621 accgaggaag cctacgacta caacggcaac acgggcaccg agaagggcga gctcgtcgcg
     1681 gcggcggcgt acggcgacgg ccgcccctgg gacgtcggcc acgaccgcgc gctgatcaat
     1741 ctcgagaacg ctttcatcga cgggcttagc gcgcaccaag cgccgttcct ctggtcggag
     1801 ttcgccgacg acgtcggaag cgctgccgac tggtacacgc tgtggatggg gccgttcttc
     1861 ttcggcaccg aacacctaac cactgcggtc tatcggctgc tcacgctcgc gccgtggatg
     1921 cgctcgagct cgggcggcac gaccggctac atccgggcca cgctgtcgcg cgtggtcccg
     1981 atcggttcgc cgggcacgag cggcacggtg actgacaagg gccgcttcac gagcgcgttc
     2041 gcgcaggccg aatggtcgac gagctcggcg acctggggcg agcagccggc gcaaacgctc
     2101 gagctatcgg ttaaggatct gttctggggc tttgcctggc tggtcatcga gaagaagggc
     2161 accggccagt tgcgaggctt ctcgcgtttc gtcgagggtg cacggaggat ccgctaatgc
     2221 ctcagctcaa gttcaccggc gatcggctcc cgatcggcag cgtaggctcc cgccaggtcg
     2281 tcgccaaccc gacgagcaag gccgccgacc cgccgattca gtacgaccgc tttgcgcgca
     2341 acggcaagca cccgaacgtc gcactgattc agcagatgca gggcgctgtg aatgcggcgg
     2401 tgatgtaccg cagcaagcaa gtgttctcga tcgcgggccg cctaaacacg gccgccccgg
     2461 tcgtgagcgc tgccgatcgg ccgcgctggc ggttcgcatt tcacacgagc ccgtacacgc
     2521 acgcgctgat ggctgtcgtc tcgatgctgc cgcccgacgt cgacatctcg ccgccaggct
     2581 acgcgaacac atcggggcgc ctcgacatcg cgaacggcgc gggatcgatc gtcgctacgc
     2641 agtcgttcta ctatggcctg catccggtgc cgcgtttcag cgggctcggc ggctacgttc
     2701 cggactggaa gcacatccga tggtttgtga agtacgtcga tggcgtggcg gccgacaccg
     2761 actacttcgg cacgttctac gatgtgaact atggccggct gcagagcgcg tgcgtgttcg
     2821 agctgccgtc gatgaccgag ttcacgaacg gctacctcgc cgagaacatc agcggacaga
     2881 gcccgatcct cgacgtctac cgcgagaagc aggcgacgat catgcgcaac ctgtggcggc
     2941 gggccggtgc gcatctgatc aattggaccg tcgacgatca gggcgcgccg ctgcagcgca
     3001 cggcgaacac gacacaggtg aacgtgatcg acctgtcgac gacgttcaac gcgtccgatc
     3061 cggccttcac gctcgtgatg gacgggaaga accggcgcac gcagtcggct ggcatcccgt
     3121 gcgtgatgaa ggcgtacggc gtgatgtcgg cgggcacgaa cggcagcgtc tacctgaaga
     3181 acggcgtcgg cacgatcgtc gcgcagatcg tgagcgcgtg gggcacgacg ccgagctggc
     3241 agaccagcgc ggtgttcaac ctgcccgcga cgaccgatcg ctatttcctg catgtggccg
     3301 ggccggcgtc gggcacgttc tcgctcggcg cgatctcggt ctacccgtac acgacctgat
     3361 caggggatcg cgatcgcaca ctcacgccag gccgccgagt cgtcgcgata cttcacgatc
     3421 gcgttgtatt cgtcggtgga catcgtgacc acgccaccgt tcgattgaac ctgccagccg
     3481 ggcgcgagcg gcggctgccc gcacatctcg acaacggtct tcccgatgtc atgcggcgtc
     3541 ggatccggct ggtcgtcggt gcaggcggcg agaacgagga aggcgagggc gaggtgtttc
     3601 atcgtgtggc ccaagatcgt gatcattcct gcccgaaagg caatgccgca aatcgttgcg
     3661 gtgggttagg agagccgcaa cctatgactg cggcttgggt atccgccagc gtgagatgct
     3721 gcgcttgacg gtcgcatgag gccgtgatcc tgaggctggg gtgacggtcg ctcgcctcgc
     3781 tctgttgctc gcgctgctcg gcggctgcag tacctacgac gcgttcgctg gtcgcatcga
     3841 cagcccgggc tcggtctatc gctgcgacga cacgccgaac ggtccgctcg agtactgcta
     3901 cttcgaggat gcagcagccg agctcagcgc ggccacgggg ctccgctgct acgagtccgg
     3961 cctgttcgat cggtgggcta ccgcgttcgg ctgctggtac cactgcgatg acccgagcac
     4021 gctcgagaac gagggcgacg gcaagggatg caacgcgcat ctcggcggct gcttctgcga
     4081 ggcgatgccg tgaccgacga gaagaagccg cgcgcgacca gccaaccggc cgcggattcg
     4141 ttcgccgcgg acgatgccag ccagttcacg ccggtaaccg acgtgctcga gctgctcgac
     4201 gccgagatcg tgctcaccga gcgcgagcgg cggatgttct tctacctgtg gaagcactcg
     4261 gcgaaccagg aaatgcgcgg ccgcaagcgc agcgactcgc aggacacgag cttgcttgcg
     4321 aagcggctcg acgagatcga cgacaagcac acgcggctcg acgtcgacct tcgcggcgac
     4381 ggcaaccgcg agcacggccg ggtggccacg ctcgagcggt tccacgcgcg gatcgtcaag
     4441 tgggcgctcg gctcgctcgt ggcgatcgcg ggcgccgcga tctcgttcgg gatctggatg
     4501 ggctcggtcc gcaccgacat cgacaacttg aagcagcgcg cgcagccgtc gcagcgctgg
     4561 cgatcgtttc tgccggactc ctcggcatca cccgacagca aggaacccac gccatgatca
     4621 cacgtctctc gagctttgca tcttccttcg ccggcgccgt cgtcctgatg agcgcgctcg
     4681 tattcggcgc catgttcaca gcctactcgt gcacgcgcgc ggcgtacgca caggagggga
     4741 gcggttcggc gatggtcgtc gacgccggct cggcatctgc tccggcgcca gatacggcgc
     4801 agcctgcacc ggcatccacg ctcgacgagc tctcggcgct gaaagcgaag tacgaggcgc
     4861 tcaagactgc acagccggac gcgaagatgc tcgcctgggc cgcgctgatc gcggctgcgc
     4921 taaagctgat gctctcgctg ttcaaccgct acgtgttcaa agaaacgaag acctggacga
     4981 aatggatcgc gctcggcgcg gccgttccta tcgcgctgct ctcgtactac gcactcggta
     5041 acgggctgtt cgcttcgctg gtctacgccg gcgcagggcc gggtgcgatc atcgtgcacg
     5101 agctgctgca gaagaaggcg ccgagctcgt cgtgagcgac cgcgacgaag aacagcgtcg
     5161 gcttatcgtc gtctacggcg accccttcgc atgcgacagc cccggccacc gtcgactcaa
     5221 ggccgaggaa accgttcggc gcgcgctggg cacgcgccag aaacagggcc gccgcctctc
     5281 ggtcgagctc gagatgcccg tgtgggtcgt cgtgctgtgc ttcgtgatgc tcgtcatcgg
     5341 gctcgtgtac ggggcgctca ggtgaccgcc gccgactaca agcgcagcct ctatcccgtc
     5401 gaggggctgc tcgccggcga gtggacggac gaaaccgcga ccgggcggcc cgcggtgtgc
     5461 tgcgcgcgct gcagcggcat ctacgacatc ccggagacgc accgaatcga cccggccggc
     5521 ttcgtgcgac ccgagctcaa gtgtggcgcc ggttgttcgg aggtcggtta tggcgtccgg
     5581 ctcctgaact acgccgacga ggtgctgcgg tgatcgtgcg cggcgttgtg cacgagctcg
     5641 tgcagggtat cgcccatgcg ctcgcctatg tcgcgatgac cgtgtttctc gcgtggctat
     5701 acgcgccgga tggggtcctg tgcttggtat agccgtcgtc atcggcgcag ttgtgctcgc
     5761 ggtggccggc gcgatgctga cgctcgcgct cgcgctccgg tctgagacgc ggcgcgcggg
     5821 cgacgaggcg aagcaacgcg ccgagctcac cgtcgagctg agggtcgcaa acgcgaacac
     5881 g
//
LOCUS       DASUFX010000801        16739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1797748, whole genome shotgun sequence.
ACCESSION   DASUFX010000801 DASUFX010000000
VERSION     DASUFX010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16739
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797748"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2149)
                     /gene="feoB"
                     /locus_tag="VFV99_32355"
     CDS             complement(<1..2149)
                     /gene="feoB"
                     /locus_tag="VFV99_32355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX5064129.1"
                     /translation="MTESCHEPGAAAHGSAHHSIAQPANRPQIAIAGNPNVGKTTLFN
                     ALTGLSAKVSNYPGITVERRTGEIKLPIGPADLHDLPGTYSLNARSAEEQIALDALAG
                     LQGERAPDAVIVCLDATQLARSAYLLLQIRELGARCVVALTMVDEAGAAAPDARALAA
                     LVGCDVIPVIARTRKGTGELLGAIDRVLRAERTAAWLWTPSTELRTHLDAVRTALPDS
                     WRATARYGAASDRADDALALWALTCLESDPSEADELVGVPDALRASVASRVGDGGRAI
                     DDETVLARWRWLDREIPKLIKKRADRSRTERIDRVLLHRGLGFVVFAGVMTVLFMSLF
                     AWADPAIDFIDRAFKFAGSGLKDLLGDGIFTDFIADGVIAGVGAVLVFLPQILLLFLF
                     LGFLEDCGYLARVAYLMDRIMRSMNLHGRAFVPMLFGFACAVPAITATRTMERRRDRI
                     LTMMVVPLMTCSARLPVYTLLIGALIPAARVTQGLLMVGMYLFSVLTSLVAAWVMSKT
                     VKPLKAKRLPFIIELPPYRFPRVRDVLRMMWSKSSMFLREAGTVILACSIALWALLYF
                     PHKPETASPEQKALQLEQSYGGQLGKAIEPAIAPLGFDWKIGVGIVGAFAAREVFVST
                     LGIVNAVGDDADERSEPLRQALREAKKPDGTKAYTPLVALSLMVFFALAMQCMSTLAV
                     VKRETQSWRWPAFLFSYMTVLAWCASFLVYQGGK"
     gene            complement(2146..2484)
                     /locus_tag="VFV99_32360"
     CDS             complement(2146..2484)
                     /locus_tag="VFV99_32360"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX5064130.1"
                     /translation="MPTSLAQIPLGKTARIASVDGPRAFRRRLLEMGLVPGVEVRVVT
                     IAPLGDPLRIEVRGGQWSIRRAEAAQIQVAAAAVQVAETARQPAPAAVDAIDKRKSAR
                     SSQTDLPATP"
     gene            2641..3189
                     /locus_tag="VFV99_32365"
     CDS             2641..3189
                     /locus_tag="VFV99_32365"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5064131.1"
                     /translation="MDSAQTLLLNQGFEPIKVISWQRAISLLFLGKVEVLEEYDRDVH
                     SVSLIIKVPAVVRLLRAFRRHARPVKFSRVNIYARDNYKCQYCGRKASISDLTYDHVV
                     PRSQGGLTEWTNIVTCCYLCNRKKGGRTPHEAGMKLLAHPTQPNWVPAVAIRISLRSV
                     PDAWRDYLYWTGELDTSAPGTS"
     gene            3125..3664
                     /locus_tag="VFV99_32370"
     CDS             3125..3664
                     /locus_tag="VFV99_32370"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX5064132.1"
                     /translation="MRGAITCTGLASSTRARPAPADVRICFVCMGNICRSPTAHGVME
                     KLVADAGIADRLVIDSAGTGAWHVGELPDERARSVAKKRGYDLTHRARKFTANDFDRF
                     DLVLAMDRDNFDNLQRLARGRTHVPPIRLLRSYDTSAPEGAEVPDPYGGEDDGFEHVL
                     DVCERACRGLLEHVRTRIA"
     gene            3718..4527
                     /locus_tag="VFV99_32375"
     CDS             3718..4527
                     /locus_tag="VFV99_32375"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5064133.1"
                     /translation="MDRQALAQALGSDVSYSSTVAGGDINDAAKVRLADGRTVFVKSH
                     ESPPAGMFAAEAAGLDWLAGGPLRVPRVIAVADDWLALEWLDLDGKPDAATFGRGLAR
                     LHGRGAPSFGLAIANFLATLPQDNTVERDWPTFYVERRLRPLFKRAKLGLDRELDQLR
                     ERPHVFGPPEPPARLHGDLWWGNVASCDGTPVILDPAVYGGHREIDLAMLALFGGLPD
                     ALVEAYDEVFPLADGWRERIALNQLYPLAAHACLFGGSYGAQVKTALGGFL"
     gene            4566..6134
                     /locus_tag="VFV99_32380"
     CDS             4566..6134
                     /locus_tag="VFV99_32380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5064134.1"
                     /translation="MNRIAVAAGALADRAVEAARTLTKNGEAIDEHQVVVERVAYAAT
                     EARVIRELADVPAQLAAAARVAEGELAASLRYRLEPIADVLKLGTLAYDEPTRAEIQQ
                     VITPSAVEAIGEATMASGGRLAWPLDETLSEVRANVRQFAEREIAPHAERIHHHDELV
                     PERFISEMANLGYFGLSIPEKYGGHELGNLAMILTTEELSRASLAAAGSLITRPEILA
                     KAILAGGTEAQKQLWLPKLASGGVMAAISVTEPNIGSDVANVSCRAERANGGFTITGA
                     KAWCTFAGRADVIALLARTDADMSKGSRGLTLFIVDKPRFPGHEFTATQDGGGTLTGK
                     ADRTPGYRGMHSFTLAFDKWFVPDAQVVGGEEGVGRGFALQMAGFAAGRLQTGGRACG
                     VTQAAIEQTARYVSERKQFGRPIGDYGLTRYHLGRMASRLVAARAIAYAAARAMDGDE
                     RQAAPVAAMAKLFSCDVAVEVTQLGQLLHGGWGYAEEYPISRYVADAQVLPIFEGVKP
                     ILALKVVGRALLGR"
     gene            6219..9614
                     /locus_tag="VFV99_32385"
     CDS             6219..9614
                     /locus_tag="VFV99_32385"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEX5064135.1"
                     /translation="MRKLFAWLGIATLFVLTSTAHAQNDVLKPYVVLILDTSGSMVCT
                     NSTCGNPTGSGPPSCGGVDNKLNHAKCAINNIVNSYGDMVFALGRFRTTLGGTTTMGT
                     FPSGCCEAGPGISGTNGCGAGPACGAATTATGVMLQLLTGLVDGNNLAAASYTNFTAN
                     SCTTGGTDPEIWDAPGACVGGGGLGDACGGATPLDGVLRGSKLYWQGNQATDGTVLWP
                     QNQAGYDPINRDPSSAVFLPKMGETTCNPNTSTCNSAGNCTGANCCCVTQCRPYVTIL
                     LTDGSETCSGDPPTAAAALLTTTPRSDQINIQSIMRSNNVVTVTFAAAHPFVVGDVVV
                     VTGVSNPTFNSGGTQPPTFTITAVTTMAPFTITYAQTGANTTSSMGNVRHAASTFQYR
                     IETKPIGFGITPGDPNIEAIAHAGGALDLPNVDEGYYAQNEADIELAMSQILADSVRA
                     ESCNERDDDCDTRVDEDFPDKGKPCMVGVGECVATGVFVCDNTGTKCNATPGTGTPEI
                     CDQKDNDCNGLVDDGITCTSCVPTGEICNNRDEDCDGRIDEGLTRACGTGTCTGVETC
                     TAGTWGGCTAQQPTTELCNGADDDCNGVCDGLSNGCSEVGGACNPNVASSCPATNSPG
                     DPGHYTGPDTAETSCSDAIDNDLDGLINDGCPTVAGTAETACNNAADDDNDGAVNDGC
                     PVPSLPIPQNICHPGTKTCAVLCNMNGNSFGACSGEVKPLATDPCDGLDNDCDNKIDE
                     GFVPQDCSTNCGVGTTQCVNGQVTCNSTPATDDTTCNNVDDDCDGQIDEDWTCANPIN
                     GMCPCGTGLVCDGLEKCVNGAVVCQGTPVGNETCNCQDEDCDTRVDEGTVCPQGSTCT
                     NCQCAFPCAQSEFPCPLGKKCDTSQGPPGYCVNDPCFNISCPPVNGHAQTCIPKPNFP
                     NEPQCVDTCTTVTCNSPLICYQPTGECRPDDCTTFPERCTGTQVCVNGQCISNPCDGV
                     TCPGDKYCLGGTCVSSCADVMCPAGQRCRQGMCETDPCGKQCPFGQQCNDATGECIED
                     PCKFRNCPQGQWCNPNDGQCEDDPCVANAIECPHEGEVCRGGSCYDPDDLRPDAAGEA
                     HVTVGGGGGCSTTSGGSSLLLGLALLLVRRRRRVPGARQGGLQ"
     gene            9623..12538
                     /locus_tag="VFV99_32390"
     CDS             9623..12538
                     /locus_tag="VFV99_32390"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEX5064136.1"
                     /translation="MPLVRALFATATLLVGAMSQLSCSVNDYCLGCDNGDGDGGMGSN
                     DADDGGGGSDDAMIDAGCVPTGPEECDGKDNDCNGLVDDGVIQGVGVLCSNQNGVCAG
                     GLTICSNGMIKCDKAGSPEVCNGLDDNCSGAPDEGDPGGGAKCGTDLGECIAGQFHCN
                     PATGMVECFGFQDHTQDPELCDGKDNDCDGNFDENVVFANPDCGPTTNVGECVIGTLS
                     CTAGTPICNGAVFPKFESCNNLDDDCNGATDEIFNKQTDIQNCGSCGNVCQPKSKTCI
                     FATNTSTSGTSCTSDSQCTNGSCVINSQPRCASGGCGFQCNAGFKDLDNNPANGCEYR
                     CFVTGLTEECDGIDNDCDGNVDEGLTPPDTCLHMAGSVCGTNLAPTCDGMAGWVCHYP
                     STFESPETKCDTLDNDCDGNVDEAQKNLAPGVGLGLACSVVPETACGADTTDDDNDGF
                     VNDGCPKVGMTAETACNDNVDDDMDGAVNDGCPAKEERGVCKSFGTYACDTNNISGPP
                     VCDITMPGQMPDANETCDAKDNDCDGKTDEGGNMGNLIGQEWVDIGGGHFMMKYEASK
                     PDATDTNQGSAPAANPADPSAFTCSRKDVQPWTNVKYQDAAAACAKIGANLCTEVQWH
                     RACSKLDAKPNTIAAPGTGTFIEAEDYMTASFGGGAESGTGCTPGTTADDDGDGRIND
                     GCPTVGPNPETACAGNADDDGDGTANDGCPAIGPHAWVEDYTSTFSGIGDMEATPNSG
                     LTVSNNPVAIAGSAPSLNYSINFTSGNNYHVWVKLFANNNQNDTVYVALNNNTASSAT
                     ASTLNTWQWVDAGSFTGTVSGTQTVHIYMADDGVKIDKLYVINGNTTAAAIGMADTIA
                     SKGGNWAFAANPNTYVAGTCNGDDYDTNAAPGDQDDILKTGALPSCKAAWGTLATDGI
                     FDLSGNVKEWTLRHAPGENPIRGGASNNTPNGISCPLNFTLADDNFFFPNIGFRCCK"
     gene            12492..13088
                     /locus_tag="VFV99_32395"
     CDS             12492..13088
                     /locus_tag="VFV99_32395"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEX5064137.1"
                     /translation="MTTSSSRTSASAAASDVTKCARCLLQQRVCLCAEIPVVTTRTRV
                     VIVRHHLERFRSSNSGRLAHLALPNSEILDHGGSGGPAQLRDLEGAWLLFPEGEPAHA
                     APVPPPKKLIVLDATWSQARRMYRKLDVLRGVPLLRLPDEPMPSARLRESPGPGRVST
                     IEAIARALRLLEGDRVATPLEQLFQVAVQRAQSTGRSV"
     gene            13100..14767
                     /locus_tag="VFV99_32400"
     CDS             13100..14767
                     /locus_tag="VFV99_32400"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5064138.1"
                     /translation="MVTTSPKARAGISISAKLILTTSVVVAVAVGAATWFSQRTIDEL
                     TQMQIANRRTSGEKSITRESELVVQAVATAVALPLAQTTYPDIKPALDAAMRDDHASG
                     DDRLQWLVVTDATGQVVAATQNGPAADKLAALDKQLDGGTKNGDVVHVRIGTGSDWVY
                     GAAIKLGDNPVGKLRIGVSTAGLEHELSKSLAVADERSKASQQRLLLIAVAVLLIGIL
                     LAALQGVGLARPIRALTVQANKIAGGDLKSRVPEGRRDELGVLAKTFNFMANQIQVLL
                     AEQRQKVSLEKEMELARQVQQAMLPPSTLDQHGYLNIVGYCAPASQCGGDWWMYRKLS
                     GGRMLFVLGDATGHGIHSAMIASTARGAVEALAAVDERLLTPEQVLRAIDQAIRMVGD
                     HNVMMTAFAAVFDSQTGALHYANAGQNFPYVMKLSDRRILESASIIAASGNPLGDRNI
                     AVEIRRGALQLRAGDVFVCFTDGVVERANKQGKLFGDRRLRTALTGQTVADSAGLVQL
                     RDKVLAALDRYAEGSQPDDDITLVFCQFDPPAASRTVSGAGYGRGAA"
     gene            14764..>16739
                     /locus_tag="VFV99_32405"
     CDS             14764..>16739
                     /locus_tag="VFV99_32405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5064139.1"
                     /translation="MKRSLALAVLLVAHVARVAHAEAPAAAVDVADLPPLPPASEEQS
                     SSATVLAASSAEEDVVVGAAKREQSLGNVASAVTVVSGDRLRRFGYRTVGEALSAVAG
                     VYLADSRLSYSLGIRGLNIPGDFNTRILVLIDGASVNESWGSFAGLGFESLVSIDDIA
                     RIELIRGPVSSVYGANAFFGIVNIVTRGAAETPRAWGRVGINSINGPVTSAGFATGNV
                     DEQVRGSVTFMNRFGDTNDLTAGDGSEIATGLSGDGSRSYMASVVGAYHGTFAQVRAY
                     HYYRDSPFAPYLSDPTVDRPFRAFDSQLLVEGGHAREVSKRLTVTGRGYANLYRYSDD
                     IVQLAPAPRFLDIGDGFAFGAELRGRYELVVPNKVGITAGTEATYVMTESRAYEEGDE
                     QCAPTGGAACVPQNFNIEGVYSEVDAQPYEWLGFTGGVRFDRNSVIDRRISPRAALFL
                     AKPEKYGAKLLYAEGFRNPSAFEAFFYDNTSFSQPQSLGAEKIRSFEAVVWAKPTGGL
                     STRLSSFYWSARDVVEQLPDPDNPMLLQFQNAAKFITMGVEAEASYRTTSGWYGFGGA
                     TYSRVGAAEDMTTPVTYGDVPNAPALVASFGVSTPLLLDRAHVSTELTYIGERPVRPD
                     TDTGDPLDAAPQWLGVNATVYVPSYKGFDITAGVR"
BASE COUNT         3025 a         5465 c         5579 g         2670 t
ORIGIN      
        1 gcttcccgcc ctgatacacg aggaagctcg cgcaccacgc gagcacggtc atgtacgaga
       61 acaggaacgc gggccagcgc cagctctgcg tctcgcgctt cacgacggcg agcgtgctca
      121 tgcactgcat cgcgagcgcg aagaacacca tcagcgacag cgcgacgagc ggcgtgtatg
      181 ccttcgtgcc gtcgggcttc ttcgcttcgc gcagagcctg acgcagcggt tcggagcgct
      241 catcggcatc gtcgccgacc gcgttgacga tgccgagtgt cgacacgaac acctcgcgcg
      301 ctgcgaacgc gccgacgatg ccgacgccga tcttccagtc gaagccgagc ggcgcgatcg
      361 cgggctcgat cgccttgccg agctggccgc catagctctg ctcgagctgc agcgcttttt
      421 gttcgggcga cgcggtctcg ggtttgtgcg ggaagtagag cagggcccac agcgcgatcg
      481 agcacgcgag gatcacggtg ccggcctcgc ggaggaacat cgacgacttg ctccacatca
      541 tgcgcaagac gtcgcgcacg cgcgggaacc gatacggcgg cagctcgatg atgaacggca
      601 gccgcttcgc cttgagcggc ttgaccgtct tcgacatcac ccacgcggcg acgagcgacg
      661 tcagcacgct gaacagatac atgccgacca tcaagaggcc ctgggtgacg cgtgcggccg
      721 gaatcagcgc gccgatgaga agcgtgtaga cggggaggcg cgccgagcac gtcatcagcg
      781 gcacgaccat catcgtcagg atgcgatcgc gccggcgctc catcgtgcgc gtcgcggtga
      841 tcgcgggtac ggcgcaggcg aagccgaaca gcatcggcac gaacgcgcgg ccgtgcaggt
      901 tcatcgagcg catgatgcgg tccatcaggt acgcgacacg cgcgagatag ccacagtcct
      961 cgaggaatcc gaggaacagg aacaacagga ggatctgcgg caggaacacg agcactgcac
     1021 cgacgccggc gatcacgccg tcggcgatga agtcggtgaa gatgccgtcg ccgagcagat
     1081 ccttgaggcc gctgccggcg aacttgaacg cgcgatcgat gaagtcgatc gccgggtccg
     1141 cccacgcgaa cagcgacatg aacaacaccg tcatcacgcc cgcgaagacg acgaacccga
     1201 ggccgcggtg gagcagcacg cggtcgatgc gctcggtgcg cgagcgatcg gcgcgcttct
     1261 tgatgagctt ggggatttcg cgatcgagcc agcgccagcg cgcgagcacg gtctcgtcat
     1321 cgatcgcgcg gccgccgtca ccgacgcggc tcgcgacact cgcccgcagc gcatcgggca
     1381 cgccgacgag ctcgtcggct tcggacggat ccgattcgag gcacgtcaac gcccacagtg
     1441 cgagcgcatc gtcggcgcgg tcgctcgcgg cgccgtaacg tgcggtcgcg cgccagctgt
     1501 caggcaacgc ggtgcgaacc gcatcgagat gcgtgcgcag ctcggtcgac ggtgtccata
     1561 gccacgcggc ggtgcgttcg gcgcggagca cgcgatcgat cgcgccgagc agctcgcccg
     1621 tgcctttacg cgtgcgcgcg atcaccggga tgacgtcgca gccgacgagc gcggcgagcg
     1681 cgcgtgcatc gggcgcggcg gcgccggcct catcgaccat cgtgagcgcg acgacgcagc
     1741 gcgcgccgag ctcgcggatc tgcagcaaca gatacgcgga gcgcgcgagc tgcgtggcat
     1801 cgaggcagac gatcacggcg tcgggcgcgc gttcgccttg cagaccggcg agcgcgtcga
     1861 gcgcgatctg ttcctcggcg ctgcgcgcgt tgagactgta cgtgccgggc aaatcgtgca
     1921 gatcggccgg gccgatcggc agcttgatct cgccggtacg ccgctcgacg gtgataccgg
     1981 gatagttcga gaccttcgcc gacaatccgg tcagcgcgtt gaacagcgtt gtcttgccga
     2041 cgttcggatt tcccgcgatc gcgatctgcg gccggttcgc cggttgcgcg atcgagtgat
     2101 gcgccgaacc gtgagccgct gcccctggct cgtggcacga ctcggtcatg gcgtggcggg
     2161 caaatcagtc tgcgacgagc gtgcggactt acgcttgtcg atcgcatcca cggctgcagg
     2221 cgccggttgg cgcgccgtct cggcaacctg gacggccgcg gctgcaacct ggatctgggc
     2281 ggcctcggca cggcgaatcg accattgccc cccccgcacc tcgatgcgca acggatcgcc
     2341 gagcggcgcg atcgtcacca cgcgaacctc gacgcccggc acgaggccca tctcgagcag
     2401 gcgccggcgg aacgcacgcg gcccgtcgac actggcgatc ctcgctgtct tcccgagagg
     2461 gatctgagcg agtgacgtcg gcatctcaga agtcgtaccc tatttgaaaa tcgttttcaa
     2521 tctcaagttc ggacacccgg ctgctatgtg cgcgagatct cacgtgagcg ccggaatgtc
     2581 gcacggagcc cacgcgatgc tacattcgag atcgagggcc gtctttccat ctcctcgtct
     2641 atggactccg ctcagacgct tctactcaat caaggcttcg agccgatcaa ggtcatctcc
     2701 tggcagcggg cgatcagtct cttgttcctc ggcaaggtcg aggtgctgga ggagtacgac
     2761 cgcgacgttc actcggtgag cctgatcatc aaggttcccg ccgtggtgcg tctgctcagg
     2821 gcattccgcc gccatgcgcg gccggtcaag ttcagccgcg tcaacatcta cgcgcgcgac
     2881 aactacaagt gtcagtactg cggtcgcaag gcgtcgatca gcgaccttac gtacgaccac
     2941 gtggtgccgc gctcgcaggg cggactgacc gagtggacca acatcgtgac gtgctgctac
     3001 ctctgcaatc gcaagaaggg tggtcgcacg ccgcacgagg cgggcatgaa attgctcgct
     3061 catcccacgc agccgaactg ggtgccggca gtcgcgattc gaatctcgct gcgctcggtt
     3121 cccgatgcgt ggcgcgatta cttgtactgg actggcgagc tcgacacgag cgcgcccggc
     3181 accagctgac gtgcgcatct gctttgtctg catgggcaac atctgccgat cgccgactgc
     3241 gcacggcgtg atggagaagc tcgtcgccga cgcgggcatc gcagacaggc tcgtcatcga
     3301 cagcgcgggc accggggcgt ggcatgtcgg tgagctaccg gacgagcgcg cacgctcggt
     3361 ggcaaagaag cgcggctacg atctgaccca tcgcgcgcgc aagttcaccg cgaacgactt
     3421 cgatcgcttc gatctcgtgc tcgcgatgga tcgcgacaac ttcgacaacc tccagcggct
     3481 cgcccgcggc cgcacgcacg tgccgccgat tcgactcctc cgctcgtacg acacatctgc
     3541 tccggaaggc gccgaagtcc ccgatccata cggtggcgag gacgacggct tcgagcacgt
     3601 cctcgatgtc tgcgaacgcg cgtgccgggg cctgctcgag cacgtacgta cgcggattgc
     3661 gtgacgcaac agttaggtac cgttgcacgc tccggacgcc tgagtacgct ccctcccgtg
     3721 gatcgccagg cactcgcaca agccctcggg agcgacgtca gctactcgag cacggttgcc
     3781 ggcggcgaca tcaatgatgc agcgaaggtc cggctcgccg acggccgcac cgtgttcgtc
     3841 aaatcgcacg agagtccgcc ggccgggatg ttcgcagccg aggccgcggg gctggattgg
     3901 ctcgcgggcg gcccactgcg cgtcccgcgc gtgatcgcag tcgccgatga ctggctcgca
     3961 ctcgaatggc tcgatctcga cggcaagccc gacgccgcga cgtttggccg cgggctcgca
     4021 cgcctgcatg gtcgcggcgc gccgagcttc ggactcgcga tcgcgaactt cctcgccacg
     4081 ctgccgcagg acaacacggt cgaacgcgac tggcccacgt tctacgtgga gcgccggctg
     4141 cgcccgctgt tcaagcgcgc aaagctcggt ctcgatcgcg agctcgatca actgcgcgag
     4201 cgtcctcacg tgttcggtcc gcccgagcca cccgcgcgtc tgcacggcga tctgtggtgg
     4261 ggcaacgtcg cgtcgtgcga cggtacgccg gtcatcctcg atcccgctgt gtacgggggc
     4321 catcgcgaga tcgatctcgc gatgctcgcg ttattcggcg gtctccccga cgcgctcgtc
     4381 gaggcgtacg acgaagtgtt cccgcttgcc gacggctggc gcgagcgcat cgcattgaac
     4441 cagctgtatc cgctcgcggc gcacgcctgc ttgttcggcg gtagttacgg tgcgcaggtg
     4501 aagaccgctc tcggcggctt cttgtgaaac ccccttatcg gcgcaaaagg cgctaagcct
     4561 tcgctatgaa caggatcgct gtcgcagccg gagcattggc agaccgcgcg gtcgaggccg
     4621 ctcgcacgtt gaccaagaac ggtgaggcga tcgacgagca ccaagttgtt gtcgagcgcg
     4681 ttgcgtacgc cgctaccgaa gcacgcgtga tccgcgagct cgcggacgta cccgcacagc
     4741 tcgccgctgc ggcgcgcgtt gcggagggtg agcttgccgc gagcttgcgc tatcggctcg
     4801 agccgatcgc cgatgtgctg aagctcggga cgctcgcgta cgacgagccg acgcgcgcgg
     4861 agatccagca agtgatcacg ccgagcgcgg tcgaagcgat cggcgaggcg acgatggcat
     4921 ccggcggccg cctcgcgtgg ccgctcgacg aaacgctctc cgaagtccgc gcgaacgtgc
     4981 gccagttcgc cgagcgcgaa atcgcaccgc acgcagagcg cattcaccac cacgacgagc
     5041 tcgtgcccga gcgcttcatc tccgagatgg cgaacctcgg ctacttcggc ctgtcgattc
     5101 ccgagaagta cggcggtcac gagctcggca acctcgccat gatcctcacc accgaggaac
     5161 tgtcgcgcgc gtcgctcgca gctgcaggct cgctgatcac gcgccccgaa atcctcgcga
     5221 aggcaatcct cgccggcggc accgaagcgc aaaaacaact ctggcttccg aagctcgcat
     5281 cgggcggggt gatggcagcg atctcggtga ccgagccgaa catcggctcc gacgtcgcga
     5341 acgtgagctg tcgcgccgaa cgtgcgaacg gcggcttcac gatcacgggc gcgaaagcgt
     5401 ggtgcacgtt cgccggccgc gccgatgtga tcgcgctgct cgctcgtacc gatgccgaca
     5461 tgagcaaggg ctcgcgcggg ctcacgctgt tcatcgtcga caaaccgcgc ttccccggcc
     5521 acgagttcac cgcgacgcag gatggcggcg gcacgctgac cggcaaggcc gaccgcacgc
     5581 cgggctatcg cggcatgcac tcgttcacgc tggccttcga taagtggttt gttccggacg
     5641 cgcaggtcgt cggcggcgag gagggcgtcg gccgcggctt cgcgctccag atggccggtt
     5701 ttgcggctgg ccgcctacag accggtggtc gagcctgtgg cgtcactcag gcagcaatcg
     5761 aacaaaccgc gcgctacgtg tcagagcgca aacaattcgg acgaccgatt ggcgattacg
     5821 gcctgactcg ctaccacctc ggtcggatgg cttctcgcct ggtcgcggcc cgcgcaatcg
     5881 cctacgcagc ggcgcgcgcg atggatggcg acgagcggca ggcggctccc gtcgccgcga
     5941 tggcgaagct tttctcgtgt gatgtcgcgg tcgaggtcac gcaactcggt cagctgcttc
     6001 atggtgggtg gggctacgcc gaggagtacc cgatcagtcg ctacgtggca gacgcgcagg
     6061 tcttgcccat cttcgaaggg gtcaagccca tcctcgcgct caaggtcgtc ggtcgcgcgc
     6121 tgctcggtcg ttgagagtga ttccgatctc ggcgagttaa ctcgcaatta ccccacccga
     6181 atatatccag cgaagcggca cctcgatacc atggtcgtat gcgcaagctc tttgcgtggc
     6241 tggggatagc cacgctgttc gtgttgacgt cgactgcgca cgcgcagaac gacgtcttga
     6301 aaccgtacgt cgtgttgatc ctcgacacct cgggttcgat ggtctgcacg aattcgactt
     6361 gcggcaatcc caccggctcc ggtccaccat cgtgcggcgg tgtcgacaac aagctgaatc
     6421 acgcgaagtg cgcgatcaac aacatcgtca acagctacgg cgacatggtc tttgcgctcg
     6481 gtcgctttcg cacgacgctc ggcggaacga cgacgatggg tacgtttccg agcggctgct
     6541 gtgaagctgg ccccggcatc agtggaacga acggctgcgg cgcaggaccc gcgtgcggcg
     6601 ccgcgaccac ggcgacgggc gtgatgctcc agctcctcac gggtctcgtc gacggcaaca
     6661 atctcgcagc ggcttcgtac acgaacttca ccgcgaactc gtgcacgaca ggcggcacgg
     6721 accccgagat ctgggatgcg cctggcgcgt gtgtgggcgg cggcggactt ggtgatgcat
     6781 gcggtggcgc gacgccactc gacggtgtgc tgcgtggatc gaagctgtac tggcagggca
     6841 atcaagcgac cgacggaacc gtgctgtggc cgcaaaacca ggccggttac gatccgatca
     6901 accgtgaccc gagcagcgct gtattcctgc cgaagatggg cgagacgacc tgcaacccga
     6961 acacgagtac ctgcaacagt gcgggcaact gcacgggcgc gaactgctgc tgcgtcacgc
     7021 agtgccgtcc gtacgtaacg atcctgctga ccgacggttc cgagacgtgc tcgggtgatc
     7081 caccgaccgc ggcggcagca ttgctaacga cgacgccgcg cagcgaccag atcaacatcc
     7141 agagcatcat gcgtagcaac aacgtcgtga cggtgacctt cgcggcggcg cacccattcg
     7201 tggtcggcga cgtggtcgtc gtcaccggcg tctcgaatcc aacgttcaac agcggtggca
     7261 ctcaaccgcc gacgttcacg atcaccgcgg tgacgacgat ggcaccgttc acgatcacct
     7321 atgcgcaaac cggggcgaac acgacgagct cgatgggcaa cgtgcgccat gctgcgtcga
     7381 cgttccagta tcgtatcgag accaaaccga tcggctttgg catcaccccc ggtgacccaa
     7441 acatcgaggc gatcgcgcac gcgggcggcg ctctggacct gccgaatgtc gacgagggct
     7501 actacgcgca gaacgaggcc gacatcgagc tcgcgatgtc ccaaatcctc gccgactcgg
     7561 tgcgcgcgga gagctgcaac gaacgcgacg acgactgcga cacgcgcgtc gacgaagact
     7621 tccccgacaa gggcaagccg tgcatggtgg gcgtgggcga gtgcgtggcg accggcgtgt
     7681 tcgtttgcga caacaccggc acgaagtgca acgcgacgcc aggcaccggc acgccggaga
     7741 tctgcgacca gaaggacaac gactgcaacg gcctcgtcga cgacggcatc acgtgtacca
     7801 gctgcgtccc gaccggcgag atctgcaaca accgcgacga agattgcgac ggccggatcg
     7861 atgaaggcct gacgcgcgcg tgcggtacgg gtacgtgtac cggcgtcgag acgtgcacgg
     7921 ctggtacgtg gggcggctgc actgcgcagc agcccacgac ggagctgtgc aacggcgccg
     7981 acgacgactg taatggtgtt tgcgacggcc tgtcgaacgg gtgctcggaa gtgggcggcg
     8041 cctgcaaccc gaacgtcgcg tcgtcgtgcc cggcgacgaa cagccccggc gaccccggcc
     8101 actacaccgg cccggatacg gcggagacga gctgctccga tgccatcgac aacgatctcg
     8161 acggcctcat caacgacggt tgcccgacgg tggccggcac ggccgagacc gcgtgcaaca
     8221 acgcggccga cgacgacaat gacggcgcgg tgaacgacgg ctgcccggtt ccgtcgctgc
     8281 cgattccgca gaacatctgc caccccggca cgaagacgtg cgcggtgctg tgcaacatga
     8341 acggcaacag cttcggcgcg tgctcgggcg aagtgaagcc gctcgcgacc gatccgtgcg
     8401 acggcctcga caacgactgc gacaacaaga tcgacgaagg cttcgtgccg caagactgct
     8461 cgacgaactg cggcgtcggc accacgcagt gtgtgaacgg tcaggtcacg tgcaacagca
     8521 cgccggccac cgacgacacg acctgcaaca acgtcgacga cgactgcgat ggccagatcg
     8581 acgaggactg gacctgcgcg aacccgatca acggcatgtg tccgtgtggc acgggcctgg
     8641 tctgcgacgg cctggagaag tgcgtcaacg gcgcggtggt ctgccaaggc acgccggtcg
     8701 gcaacgagac ctgtaactgc caggatgagg actgcgacac gcgagtcgat gaaggcacgg
     8761 tgtgcccgca gggctcgaca tgtacgaact gccagtgcgc gttcccgtgc gcgcagagcg
     8821 agtttccgtg cccgctcggc aagaagtgcg acacgagcca gggcccgccc ggctactgcg
     8881 tcaatgaccc gtgcttcaac atcagctgtc cgccggtgaa cggtcacgcg cagacgtgca
     8941 tcccgaagcc gaacttcccg aacgagccgc agtgtgtcga cacctgcacg accgtgacgt
     9001 gcaacagccc gctcatctgt taccagccga ccggcgagtg ccgcccggac gactgcacca
     9061 cgttccccga gcgctgcacg ggcacccagg tctgcgtcaa cggtcagtgc atctcgaatc
     9121 cgtgtgacgg tgtgacgtgc ccgggcgaca agtactgcct cggcggtacg tgcgtctcga
     9181 gctgtgcgga cgtgatgtgc ccggcgggtc agcgttgccg ccagggtatg tgcgagaccg
     9241 atccgtgcgg caagcagtgt ccgttcggtc agcagtgcaa cgacgccacc ggcgagtgca
     9301 tcgaggatcc gtgcaagttc cgcaactgcc cacagggtca gtggtgcaac ccgaacgatg
     9361 gccagtgcga ggatgatccg tgtgttgcga acgcgatcga gtgtccgcac gagggtgagg
     9421 tctgccgcgg cggcagctgc tacgacccgg atgacctgcg tcctgacgcg gcgggcgaag
     9481 cgcacgtcac cgtcggtggt ggcggcggct gtagcacgac gagcggcggc agcagcctgc
     9541 tgctcggtct cgcgctgctg ctcgtgagac ggcgacggcg tgtgcccggc gcacgtcaag
     9601 gaggtctcca gtgattcgcc ggatgcctct cgtgcgcgcg ttgttcgcga cggcgacgtt
     9661 gctcgtcggt gcgatgtctc agctgtcgtg cagcgtcaat gactactgtc tcggttgtga
     9721 caacggcgac ggcgatggcg gcatgggtag caacgacgcc gacgatggtg gcggcggcag
     9781 cgacgacgcg atgatcgatg cgggctgcgt accgacgggc ccggaggaat gcgacggcaa
     9841 ggacaacgac tgcaatggtc tcgtcgacga tggcgtgatc cagggcgtcg gcgttctgtg
     9901 ctcgaaccag aacggcgtgt gcgcaggtgg tctgacgatc tgcagcaacg gcatgatcaa
     9961 atgcgacaag gcgggctcgc ccgaggtctg caacggcctc gacgacaact gcagcggtgc
    10021 gccggacgaa ggtgacccgg gtggtggcgc gaagtgcggc accgacctgg gcgagtgcat
    10081 cgccggtcag ttccactgca acccggcgac cggcatggtc gagtgcttcg gcttccaaga
    10141 ccacacgcag gatcccgagc tctgcgacgg taaggataac gactgcgacg gcaacttcga
    10201 cgagaacgtc gtgttcgcca accccgactg cggaccgacc acgaacgtgg gcgagtgtgt
    10261 gatcggcacg ctgagctgca cggctggcac gccgatctgc aacggcgcgg tgttcccgaa
    10321 gttcgagagc tgcaacaacc tcgacgacga ctgcaacggc gcgaccgacg agatcttcaa
    10381 caagcagacc gacatccaga actgcgggtc gtgcggcaac gtttgtcagc cgaagagcaa
    10441 gacgtgcatc ttcgcgacca acacgtctac cagtggcacg tcgtgcacgt cggattcgca
    10501 gtgcacgaat ggctcgtgcg tcatcaactc gcagccgcgc tgcgcgtcgg gtggatgcgg
    10561 cttccagtgc aatgcaggct tcaaggacct cgacaacaac ccggcgaacg gttgtgagta
    10621 tcgctgcttc gtcaccggtc tcaccgagga gtgcgacggc atcgataacg actgcgacgg
    10681 caacgtcgac gagggactga cgccaccgga tacgtgtctg catatggcgg gcagcgtgtg
    10741 cggcacgaac ctcgcgccga cgtgcgatgg catggccggc tgggtctgcc actacccgtc
    10801 gacgttcgag tcgcccgaga cgaagtgcga caccctcgat aacgactgcg acggcaacgt
    10861 cgacgaggcg cagaagaacc tggcgccggg tgttggcctc ggtctcgcct gcagcgtcgt
    10921 gccggagacg gcgtgtggtg ccgacacgac cgacgacgac aacgacggct tcgtgaacga
    10981 cggttgcccg aaggtcggca tgaccgccga gaccgcatgt aacgacaacg ttgacgacga
    11041 catggatgga gccgtcaacg acggctgccc cgcgaaggaa gagcgcggcg tctgcaagag
    11101 cttcggcacg tacgcatgcg atacgaacaa catctccgga ccgccggtct gcgacatcac
    11161 gatgccgggg cagatgcctg acgcgaacga gacctgtgac gcgaaggaca acgactgcga
    11221 cggcaagacc gacgagggtg gcaacatggg caacctcatc ggccaggagt gggtcgacat
    11281 cggcggcggc cacttcatga tgaagtacga agcgtcgaag cccgacgcga cggacaccaa
    11341 ccagggcagt gcgccagcgg ccaatccggc ggacccgagc gcgttcacgt gctcgcgcaa
    11401 ggacgtgcag ccgtggacga acgtcaagta tcaggacgcg gctgcggcct gcgcgaagat
    11461 cggcgcgaac ctgtgtaccg aggtgcagtg gcaccgagcg tgctcgaagc tcgacgcgaa
    11521 gccgaacacg atcgcggcgc cgggtaccgg tacgttcatc gaagccgagg actacatgac
    11581 ggcgtcgttc ggtggtggcg cagagtcggg cactgggtgc acgccgggca ccaccgccga
    11641 tgacgacggc gatggccgca tcaatgacgg ctgtcccacc gtaggtccga accccgagac
    11701 ggcctgcgcc ggcaacgcgg acgacgacgg cgacggtaca gccaacgacg gttgcccagc
    11761 gatcggcccg cacgcgtggg tcgaggacta cacgagcacg ttctcgggca tcggcgacat
    11821 ggaagcgacg ccgaatagcg gtctcacagt gtcgaacaac ccggtggcga ttgcagggtc
    11881 agcgccttcg ctgaactact cgatcaactt cacgagcggc aacaactacc acgtctgggt
    11941 gaagctcttc gcgaacaaca accagaacga caccgtctac gtcgcgctga acaacaacac
    12001 cgcgtcgtcg gcgacggcga gcacgctcaa cacctggcag tgggtcgatg ctggctcctt
    12061 cactggtacg gtgtcgggga cgcagactgt ccacatctac atggccgatg acggtgtgaa
    12121 gatcgacaag ctctacgtca tcaacggcaa caccaccgca gccgcgatcg gcatggcgga
    12181 cacgatcgcg agcaagggtg gcaactgggc gttcgctgcg aacccgaata cgtatgtcgc
    12241 gggcacgtgc aacggcgacg actacgacac gaacgctgcc cccggcgatc aggacgacat
    12301 cctgaagacc ggcgcgctgc cgagctgcaa ggcggcgtgg gggacgctgg cgaccgacgg
    12361 catctttgac ctgtcgggca atgtgaagga gtggacgctg cgtcacgcac caggtgaaaa
    12421 cccgatccgt ggcggcgcgt cgaacaacac gccgaacggc atcagttgtc cgctaaactt
    12481 cacgctcgcc gatgacaact tcttcttccc gaacatcggc ttccgctgct gcaagtgacg
    12541 taacgaagtg tgcgcgctgc ttgctccagc agcgcgtctg tctatgcgcg gagattccgg
    12601 tggtcacgac gcggacgcgc gtcgtgatcg tccgtcatca cctcgagcgg tttcggtcgt
    12661 cgaattcggg acggctcgcc catctcgcgc tgccgaatag cgagatcctc gatcacggtg
    12721 gtagcggcgg gccggcgcag ctgcgcgacc tcgagggcgc gtggctgctg tttcccgaag
    12781 gggaaccggc gcacgccgcc ccggtaccac cgccgaagaa gctgatcgtg ctcgacgcga
    12841 cctggtcgca agcacgccgc atgtaccgca agctcgacgt gctgcgcggc gtaccgctct
    12901 tgcgacttcc cgacgagccg atgccgtcgg cacggctgcg cgagtcgccg ggacccggcc
    12961 gcgtgtcgac gatcgaggcg atcgcacgcg cgctccgcct gctcgaaggc gatcgagttg
    13021 caacacccct cgagcagttg ttccaggtcg cggtccagcg cgcccagtcg accggccggt
    13081 cggtgtaagc tcagtaggct tggttaccac tagccccaag gctcgcgccg ggatttcgat
    13141 ctcggcgaag ctgattctca cgacgtcggt cgtggtggcg gtggctgtcg gtgcggcgac
    13201 gtggttctcg cagcgcacga tcgacgagct cactcagatg cagatcgcga atcgccggac
    13261 cagtggcgag aagtcgatca cgcgcgaatc cgagctggtc gtccaggcgg tcgcgacggc
    13321 ggtcgccttg ccgctcgcgc agacgaccta tcccgacatc aagcccgctc tcgatgctgc
    13381 gatgcgcgat gaccacgcaa gtggcgacga ccgcctgcag tggctcgtcg ttaccgacgc
    13441 gaccgggcag gtggtcgcgg ccacgcaaaa cggaccagcg gccgacaagc ttgcagctct
    13501 cgacaagcag ctcgatggtg ggacgaagaa cggcgacgtc gtccacgtcc ggatcgggac
    13561 cggttcggat tgggtgtacg gcgcggcaat caagctcggc gataacccgg tcggtaagct
    13621 gcgcatcggt gtgtcgacag ccggcctcga gcacgagctc tcgaagtcac tcgcggtcgc
    13681 agacgagcgc agcaaggcgt cgcagcagcg attgctgttg atcgcggtcg cggtgctctt
    13741 gatcggtatc ctgctcgcgg cgctgcaggg cgtcgggctc gcgcgaccga ttcgcgcatt
    13801 gaccgtacag gcgaacaaga tcgcaggcgg cgatctgaag agccgcgttc cggaaggccg
    13861 ccgcgacgag ctcggtgtgc tcgcgaagac gttcaacttc atggcgaacc agatccaagt
    13921 cctgctcgcc gagcagcgcc agaaggtgtc gctcgagaag gagatggagc tcgcgcgcca
    13981 ggtccagcaa gcgatgctgc cgccctcgac gctcgatcag cacggctacc tcaacatcgt
    14041 cggctactgc gcgcccgcat cgcagtgcgg cggtgactgg tggatgtatc gcaagctgtc
    14101 gggaggccgg atgctgttcg tgctcggcga tgcgacgggc cacggcattc actcggcgat
    14161 gatcgcgtcg actgcgcgcg gtgcggtcga agcgcttgcc gccgtcgacg aacggctgct
    14221 cacgcccgag caggtgctgc gcgcgatcga tcaggcgatc cggatggtcg gcgatcacaa
    14281 cgtgatgatg accgcgttcg ccgcggtgtt cgattcgcag accggcgcac tgcactatgc
    14341 gaacgccggg cagaacttcc catacgtgat gaagcttagc gaccgccgga tcctcgagag
    14401 cgcgagcatc atcgcggcga gcggcaatcc gcttggcgat cggaacatcg ccgtcgagat
    14461 ccggcgcggg gcgctgcagc tgcgggcggg tgacgtgttc gtatgcttca ccgacggtgt
    14521 cgtcgagcgc gcgaacaagc aaggcaagct gttcggcgat cgccggctcc gcactgcact
    14581 gaccggtcaa accgtcgccg acagcgccgg gctcgtgcag ctgcgcgaca aggtgcttgc
    14641 cgcgctcgac cgctacgccg aaggctccca gcccgacgac gacatcacgc tcgtgttctg
    14701 tcagttcgat ccgcccgctg ccagtcggac ggtctcgggc gccggctacg gccgaggcgc
    14761 cgcatgaagc gttcgctcgc tctcgcggtg ttgctcgttg cccacgtcgc ccgcgtcgca
    14821 cacgcggaag caccggccgc tgcggtcgac gttgcggact tgccgccgct gccaccggcg
    14881 tcggaggagc aatcgagctc ggccaccgtg ctcgccgcgt cgtcggctga agaagacgtc
    14941 gtcgtcggtg ccgcgaagcg cgagcagagt ctcggcaacg tggcgtcggc agtcaccgtc
    15001 gtcagcggcg atcgcttgcg gcggttcggt taccgcaccg tcggcgaagc gctgtcggca
    15061 gttgccggcg tctacctcgc cgacagccgg ctctcgtact cgctcggcat tcgcggcctg
    15121 aacatccccg gcgacttcaa cacgcgcatc ctcgtcttga tcgatggcgc gagcgtcaac
    15181 gaatcgtggg gctcgttcgc cggcctcggc ttcgagagcc tggtgtcgat cgacgacatc
    15241 gcgcgcatcg agctgattcg cggcccggtg tcatcggtct acggtgcgaa cgcgttcttc
    15301 ggtatcgtca atatcgtcac gcgcggtgca gccgagacgc cgcgcgcctg gggacgtgtc
    15361 ggcatcaact cgatcaacgg tccggtcacg agcgctggct tcgcgacggg caacgtcgac
    15421 gagcaggtcc gcggctcggt cacgttcatg aatcggttcg gtgacacgaa cgatctcacc
    15481 gcgggcgatg gaagcgagat cgcgaccggt ctctccggtg acgggtctcg ctcgtacatg
    15541 gcgtcggtcg tcggcgcgta tcacggcacg tttgctcagg tgcgcgcgta tcactactac
    15601 cgcgattcgc cgttcgcgcc atatctctcc gacccgacgg tcgaccggcc gtttcgcgca
    15661 ttcgacagcc agttgctcgt cgagggcggt cacgcgcgcg aagtgtcgaa gcggctgacg
    15721 gtcacaggcc gtggctacgc gaacctctat cgctacagcg acgacatcgt gcagctcgca
    15781 ccggcgccgc gcttcctcga tatcggcgac ggcttcgcat tcggtgccga gctccgcgga
    15841 cgctacgagc tggtggtgcc gaacaaagtc ggcatcaccg ccggtaccga agcgacgtac
    15901 gtgatgaccg agagccgtgc gtacgaagaa ggtgacgagc agtgcgcgcc tacgggtggc
    15961 gcagcgtgcg tgccgcagaa cttcaacatc gaaggcgtgt actccgaagt cgatgcgcag
    16021 ccctacgagt ggctcggctt taccggcggc gtgcgcttcg accgcaactc ggtgatcgat
    16081 cgccgcatct cgccgcgcgc cgcgttgttc ctcgcgaaac cggagaagta cggcgccaag
    16141 ctgctctatg cggaaggctt ccgcaacccg agtgcgttcg aggcgttctt ctacgacaac
    16201 accagcttct cgcagccgca gagccttggc gcggagaaga tccgcagctt cgaagccgtc
    16261 gtgtgggcga agccaaccgg tgggttgtcg acgcgcctgt cgtcgtttta ctggagcgcg
    16321 cgcgacgtcg tcgagcagct gcccgatccg gacaacccga tgctgttgca gttccagaac
    16381 gcggcgaagt tcatcaccat gggcgtcgag gccgaggcga gctatcgcac gacgagcggc
    16441 tggtacggct ttggcggtgc gacgtactcg cgcgttggcg ccgccgagga catgacgact
    16501 ccggtgacgt acggcgacgt gccgaatgcg cctgcgctcg tcgcgtcgtt cggcgtctcg
    16561 acgccgctct tgctggatcg cgcgcacgtc tcgacggagc tgacgtacat cggcgagcgg
    16621 ccggtgcgtc cagataccga taccggcgat ccgctcgacg cggcgccgca gtggctcggc
    16681 gtcaacgcga cggtgtacgt gccgagctac aagggcttcg acatcaccgc gggtgtccg
//
LOCUS       DASUFX010000802        14067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1518158, whole genome shotgun sequence.
ACCESSION   DASUFX010000802 DASUFX010000000
VERSION     DASUFX010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14067
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518158"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFV99_32410"
     CDS             <1..786
                     /locus_tag="VFV99_32410"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEX5064140.1"
                     /translation="VQEGSTSVNRGCATADLSPERMAEVDQEFAAYKASHDVAYATKN
                     VNVHVHIITNTSGQGKPTSTQINNQITVLDNAYASSGWGFTLVSTDTTANNSWYTCSG
                     GTCETQMKNALRIGGAADLNMYFNNMGGGLLGWATFPSSYKSSPKMDGVVILTASLPG
                     GSAAPYNLGDTATHEVGHWMGLYHTFQGGCNGSGDGVSDTPAEKSAAFGCPTGRDTCT
                     GKRAPGLDPITNFMDYTDDSCMDRFSAGQTSRMASMEATYRGL"
     gene            861..1115
                     /locus_tag="VFV99_32415"
     CDS             861..1115
                     /locus_tag="VFV99_32415"
                     /inference="COORDINATES: protein motif:HMM:NF013083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEX5064141.1"
                     /translation="MSDLPQRFADAQVRIKPVTGLGNDTMLELYALYKQATTGDVDGG
                     RPGMMDLRGRAKYDAWAKRKGMTKDAAMQAYIELVAKHVK"
     gene            1115..1912
                     /locus_tag="VFV99_32420"
     CDS             1115..1912
                     /locus_tag="VFV99_32420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064142.1"
                     /translation="MRAIWIAVAALALACACKDKKPPVQHDEPATQTRQRPADRPALE
                     TPDVTGPGLVPAVGLGPKVTFSKTEIKVDDESVAPVRPDGWIDASRVEVLTRTLEGKA
                     NSDAPIAITLDAMVPYRRVGQLLDTLKRAGFRNLALLTGSGGQMIPIELPDSAEINGA
                     GLRPVVTLERKQVSLWSASGLEGTRARPKLTFGLGETRSFAPLTRALADIVQKRWPTG
                     KRPAEDRTIIIQPDGNQPAEVLLQLLASVRADGSVELFPNIFLTGGQ"
     gene            1913..3022
                     /gene="corA"
                     /locus_tag="VFV99_32425"
     CDS             1913..3022
                     /gene="corA"
                     /locus_tag="VFV99_32425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX5064143.1"
                     /translation="MLARIYQDDQVKTVRDVAEIRAAIDAKLPIWIELESQCDEGEQL
                     MLEVFNIHPLTVEDIWQVRAAPKLEDYRNYLYVIVHGVHSVKRGQIDLIELDVIIGKT
                     FVITHDPTGETTKDVVDELERDPSLLCKGPAWLAHAILDNAVDRYLPIVDQLDEDIEK
                     LEMAALLKAGTPKGPAVLTRILQYRRLLQDLRRMSIHQREILLRLARGEFDEIPREVV
                     PFFRDVFDHFVRINDLLDGFRDLITSALEAYLGVQNNRMGEVMKTLTLISTVMLPLTF
                     VVGLYGMNFKHMPELNWLYGYPLALALMGTIAFGILWWFRRKGWIGSTDLPEERPRGT
                     TRKAKSVVAKPATAKPAPAKSAAAKPAAKSEPSSD"
     gene            complement(2997..4499)
                     /locus_tag="VFV99_32430"
     CDS             complement(2997..4499)
                     /locus_tag="VFV99_32430"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HEX5064144.1"
                     /translation="MRISSNWLAILTLVSACATSADPNAIDMYIQSLPYLPVDPPQVV
                     PGGQSAAARDGDYSCTTENLKETRQLDRIVAYAANSDSLWPGAIVSADSVVSGLFTQV
                     VLPRAPATFSVSLENLGGTKKATIAEPSLSAYRDALASVLQSEITGSTPANIYSEIEE
                     VHSEQQLNMALGVQASWALGLASLKTSFDWQSQNVRSRYVVRYTQAYYTVDLDAPASP
                     SALFANSVSLEDVKAKMNEEHPPAYVSSVTYGRMIVFTFESQYSASELGAALDFAYSG
                     GVEVKGDVSVTYKDILSSSKITAFILGGDAGTAVKTIDSYDSLIDFIKTGGNYTRESP
                     GAPIAYKLSYLKDNSPARMSFTTDYEVKDCVRVAQKIKVTLNSIAVDDDGGDGGGALE
                     IYGLVNATGTTTATLFNKSEGNRVTIQEGKSIGNASQPLGEAVIEVKPQAGQSIRLRA
                     NLTEHDPLFNDSLGDETVSAAFETGWRKDVTVLLTGSNARVRVNLSLAPI"
     gene            4680..5144
                     /locus_tag="VFV99_32435"
     CDS             4680..5144
                     /locus_tag="VFV99_32435"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5064145.1"
                     /translation="MRVLCSAIVILCISLTSAHARTATGYRHGQKTRIKLVEVGGVEL
                     EAATATAFRAMAKAAAKKGIELAIRSGFRSHEKQKELYRDYRRGWGHLAARPGYSNHQ
                     SGRALDIYIDDYRVYEWLKEHARDYGFRRTVRREAWHWEYVGGERAARRATS"
     gene            5205..5390
                     /locus_tag="VFV99_32440"
                     /pseudo
     CDS             5205..5390
                     /locus_tag="VFV99_32440"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5387..5641)
                     /locus_tag="VFV99_32445"
     CDS             complement(5387..5641)
                     /locus_tag="VFV99_32445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064146.1"
                     /translation="MPVEDLATIEYKLAKRGFKQQDVYHHECPSCKEVAVRIYGIGGK
                     LGGRDIRLCLACGACRSFRAAAGMQDRVEEPGFDLQAFLR"
     gene            complement(5660..6412)
                     /locus_tag="VFV99_32450"
     CDS             complement(5660..6412)
                     /locus_tag="VFV99_32450"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5064147.1"
                     /translation="MIDVKSKVSEAEWNLRVDLAACYRLVALYGWDDLIFTHISARIP
                     GEGHQFLINPYGMTFDEVTASSLVKIDLEGKKLMDSPYEINPAGFTIHSAIHAAREDA
                     KCVLHTHSINGVAVSATKGGVLPVSQQSIFVLSSLAYHDYEGVALLEGEKPRLVRDLG
                     EKSFFMLRNHGLLTVGPSIPDAFLLMYIFESACTIQLRAQAASSELIPIDQKIVAGAQ
                     MMAKQVTRSAGGGLAWPGLLRKLDRLDRSYRD"
     gene            6419..7144
                     /locus_tag="VFV99_32455"
     CDS             6419..7144
                     /locus_tag="VFV99_32455"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5064148.1"
                     /translation="MLREGMGRGEDVAKAIAADLQGKSQPGRAKNEKAYLKSDLDHVG
                     VPMPAIRSTVVAALRDAKDLTRAELLAAIAALWDGVHEHRMAAIELLVKRQKLLTPRD
                     AKLVEKLLRDSRTWAYVDVLATHGMGPLVVADPSLERTLDRWAKDDNFWMRRSAMLAL
                     LGPLRTGGGDFARFGRYADAMLDEKEFFIRKAIGWILRETSRKQPKLVFDWLLPRAAR
                     ASGVTMREAVKYLTPAQRARLGR"
     gene            complement(7141..7575)
                     /locus_tag="VFV99_32460"
     CDS             complement(7141..7575)
                     /locus_tag="VFV99_32460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5064149.1"
                     /translation="MTPFRYLLRVRYSECDAQGIVFNARWADYVDIAVGEYSRALFGS
                     VDPAVTGIDWKLVKQTVEWKASARYDDILDCRVSTIRVGTTSFTIATELHRFNGPLLA
                     TVETVYVAFDRHGAKTAVADDYRKALERGAPHVIVDHAAALR"
     gene            complement(7585..7833)
                     /locus_tag="VFV99_32465"
     CDS             complement(7585..7833)
                     /locus_tag="VFV99_32465"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5064150.1"
                     /translation="MDILTWLIVGLIAGVLAGLIVGGYGLLADIVIGIVGAFIGGFIF
                     RAAGWSAPWGGLAGTIFVAFIGAVVLLIILHLFNRRAV"
     gene            complement(7936..8988)
                     /locus_tag="VFV99_32470"
     CDS             complement(7936..8988)
                     /locus_tag="VFV99_32470"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX5064151.1"
                     /translation="MRALLLLIALTACKGSTKSATPSGDYCRGESHYVAAPTPLGTLG
                     GSSVRPTNTFSIVARDPVTGDLGVAVQSHWFSVGSAVTWAEPGVGAVATQAFAEPGYG
                     PKGLALMRDGVAPADAIAQLTAADPQSATRQLGFVDAQGRVASHTGAKCIAFASSKTG
                     DGYTVQANIMANDKVVPAMAQAFETTKGDLADRMLAALDAAQATGGDLRGCQSAAILI
                     VSGNKTDKPWAEKKLDLRVEDAAEPLKELHRLVTLARAYDHMNKGDAALEKNDIAGAA
                     EHYTTATRMEPNNTEMAFWAGVSLAGGGQYDAGLPLVRRALKSDPVYVELLKRVVDPP
                     AVISKENAERLLREAR"
     gene            9017..9667
                     /locus_tag="VFV99_32475"
     CDS             9017..9667
                     /locus_tag="VFV99_32475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064152.1"
                     /translation="MRCLVVLALAACGGTPKPASTESGRPPYLALLQHGQRWSLPIET
                     VNGEHRGDAWAATSTERGALTCEVADVKTIADATMSHVQCAPPYAGLLVVGYWVATPA
                     GLYHPALPVDDVDELTLLGENDLLLTAKPKEREHSQATAGMQHAIEAFRFKQSWCMRD
                     AVVTEEDRRHFTLCVDGSGMTGGGELVVSGTDHSWHRSTFGVVADDPDDPTTGHDD"
     gene            complement(9655..9822)
                     /locus_tag="VFV99_32480"
     CDS             complement(9655..9822)
                     /locus_tag="VFV99_32480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064153.1"
                     /translation="MLGGHDHHYLHVVWVVASAFGVAALVVLAVIYRRAARRHDIEQA
                     ERIIAEHDQSS"
     gene            complement(9825..10460)
                     /locus_tag="VFV99_32485"
     CDS             complement(9825..10460)
                     /locus_tag="VFV99_32485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064154.1"
                     /translation="METNRLSQLMTLTCGGHLARPVTRAQETMLAVLALVAALALSSL
                     WGVAAGSASPALAAANAYKVPMVILLSALTALPAGLVALRLTNAKLPAVELASAFALA
                     IFGGTLVLATLAPLVAIYYHTSSKAGVPIALGSVFIALATATLLFGRAVRRRVLAQTG
                     SETSGRAASLLAVGVLVTLFVAALLQYVAVASPIISAVTRFNGGFDTMFGN"
     gene            10604..10951
                     /locus_tag="VFV99_32490"
     CDS             10604..10951
                     /locus_tag="VFV99_32490"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX5064155.1"
                     /translation="MLTAQQEAWNRGDLEAYMSGYLNSPELVFTSASKIRRGWQETYD
                     KYKTKYGSDKATMGKLAFEILGVQQLGADGAIVLGRWTLTETPNAGSGVFSVALRRTE
                     RGWLVVHDHTSSD"
     gene            10988..11980
                     /locus_tag="VFV99_32495"
     CDS             10988..11980
                     /locus_tag="VFV99_32495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064156.1"
                     /translation="MKPNALLLFPLLAACTDLDMVEITEQDVVLPATKVCAQDVVLHP
                     TSDYVALASLHQDFPNGGARLISATMLGTVTSSSGSASPMQGLRISCVGPENRMIQTT
                     ENAIKGGGSLATKASLTITGPGSWDCTLGGITMHTGAALAITGSETCLSSAVSDAQDW
                     PHWPADSANVGDGQRVRAGYSTYVLKHDYALASDVATFGVRGNIELTDITGDASGTNI
                     AECMDSTADPTAPAYPRLYISAQQKSTTGYCGSRVVGPYLNTTISAKSHHKKVYPVLD
                     NVPVSNALGCLPTFEVKVMVSALAGGNALCFHAARYSNVVVVPQSYDGPVNALN"
     gene            complement(11977..>14067)
                     /locus_tag="VFV99_32500"
     CDS             complement(11977..>14067)
                     /locus_tag="VFV99_32500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX5064157.1"
                     /translation="PQDIIFDPNILTVATGMEEHNNYALDFINATRWIKQNLPGAKVS
                     GGVSNISFSFRGNNKVREAMHSAFLFHAIKAGMDMGIVNAGMLEVYEDVPQDMLVKVE
                     DVLLNRRPDATEILVDYAEQFKGQTGAKKQDVDLAWREAPVEKRLEHALLRGITDFID
                     EDTQEALDKLKVPLKVIEGPLMDGMGVVGDLFGAGKMFLPQVVKSARVMKKAVAILEP
                     LMEAEKQRTGMRAAKGKMVIATVKGDVHDIGKNIVGVVLRCNGYEVTDLGVMVPAHKI
                     LDTAAELDADLVGLSGLITPSLDEMIGVASEMTRRNMKVPLLIGGATTSGKHTAVKIA
                     PAYAGSTVHVPDASLAVGVLGKLMSGERENYLAEVAAKQASQREAFVATQKRPLLSLE
                     DARARRMKPDFTTVAKPAFTGIKNVDLDLAELAKWIDWSPFFHTWELSGRYPAILDDP
                     KKGDAARKVFADGQALLQRIVGGKLLRARATYGFFPAHAEGDDLVVGDKRFPMLRQQE
                     DKDVCLSLADFVAPTPGKDYVGAFIVTAGLGVDELAGSFEAQHDDYSAIMTKALADRL
                     AEAAAEHMHHRARVEWGYGASEGLTHDDIIAEKYRGIRPAFGYPACPDHTPKGTLFAL
                     LDNAQHHGVTLTESYAMLPTASVSGLYFAHPDARYFAVGRMQADQVTDYARRLGITPT
                     EVARMMPSLVPSGV"
BASE COUNT         2458 a         4549 c         4693 g         2367 t
ORIGIN      
        1 gttcaggaag gctcgaccag cgtgaaccgt ggctgcgcca cggcggacct ctctcccgag
       61 cggatggcgg aagtcgacca agaattcgcc gcgtacaagg cgtcgcacga cgtcgcctac
      121 gcgaccaaga acgtcaacgt ccacgtccac atcatcacga acacgtcggg ccaaggtaag
      181 ccgacgtcga cgcagatcaa caaccagatc accgtgctcg acaacgcgta cgcgagctcg
      241 ggttggggct tcacgctcgt gtcgaccgac acgacggcga acaacagctg gtacacgtgc
      301 agcggtggca cgtgcgagac gcagatgaag aacgcgctgc gcattggcgg cgcggcggat
      361 ctgaacatgt acttcaacaa catgggcggc ggcctgctcg gctgggcgac gttcccatcg
      421 agctacaaga gctcgcccaa gatggacggc gtcgtgattc tcacggcctc gctgcccggt
      481 ggcagcgcgg cgccgtacaa cctcggcgac accgcgaccc acgaggtcgg ccactggatg
      541 ggcctctatc acacgttcca gggcggctgt aacggcagcg gcgacggcgt gagcgacacg
      601 ccggccgaga agagcgcggc gttcggctgt ccgacgggcc gcgacacttg cacgggcaag
      661 cgcgcgccgg gcctcgaccc gatcacgaac ttcatggact acaccgacga ctcctgcatg
      721 gaccgcttca gcgcgggcca gacctcgcgc atggcgtcga tggaagcgac gtaccgcggg
      781 ctctaagcaa aacgcggtgg cgaaacgagc agcgcggcgc gtgagtcgcg ctgcttgcgt
      841 tttcggccta tcgtcgcggc atgtctgatt tgccccagcg gtttgccgac gcgcaggtcc
      901 ggatcaagcc ggtcactggg ctcggcaacg acacgatgct cgagctgtac gcgctgtaca
      961 agcaggccac gaccggcgac gtcgacggcg gccgcccggg catgatggat ctgcgcggcc
     1021 gcgcgaagta cgacgcatgg gccaagcgca agggcatgac gaaggacgca gcgatgcagg
     1081 cgtatatcga gcttgtcgcc aagcacgtga agtgatgaga gcaatctgga tcgcggtcgc
     1141 cgcgctcgcg cttgcgtgcg cgtgcaagga caagaagccg ccggtacagc atgacgagcc
     1201 ggcgacgcag acgcgccagc gtcccgccga ccggcccgcg ctcgagacgc cggacgtcac
     1261 cgggccgggt ctcgtcccgg cggtcgggct cgggccgaag gtcacgttct cgaagaccga
     1321 gatcaaagtc gacgacgaga gcgtcgcgcc ggttcggccc gacggctgga tcgacgcctc
     1381 gcgcgtcgag gtgctgacgc gcacactcga aggtaaggcg aacagcgacg cgccgatcgc
     1441 catcacgctc gatgcgatgg tgccgtatcg gcgggtcggt cagctgctcg atacgctcaa
     1501 gcgcgcgggc tttcgcaacc tcgcgctgtt gaccggtagc ggcgggcaga tgattccgat
     1561 cgagctgcca gactccgcgg agatcaacgg agccgggttg cggccggtcg tcacgctcga
     1621 gcgcaaacaa gtctcgctgt ggtcggcatc gggtctcgaa ggtactcgcg cgcgcccgaa
     1681 gctcacgttc gggctcggcg agacccggag cttcgcgccg ctgacgcgcg ccctcgccga
     1741 catcgtgcag aagcgttggc ccaccggcaa acgtcccgcc gaggaccgca cgatcatcat
     1801 ccagccggac ggcaaccagc cggccgaagt gttgctgcag ttactcgcat cggtccgtgc
     1861 cgatggctcc gtggagctgt ttccgaatat cttcctgacg ggcgggcaat agatgttggc
     1921 gcggatctac caggacgatc aggtcaagac ggtgagagac gtcgccgaga tccgcgcggc
     1981 gatcgacgcg aagctgccga tctggatcga gctcgagtcg caatgcgacg agggcgagca
     2041 gctgatgttg gaggtcttca acatccaccc gctgaccgtc gaagatattt ggcaggtgcg
     2101 cgccgcgccg aagctcgagg actaccgcaa ctacctctac gtgatcgtgc acggcgtgca
     2161 ctcggtgaag cgcggccaga tcgatctcat cgagctcgac gtcatcatcg gtaagacgtt
     2221 cgtgatcacg cacgatccca ccggcgagac gacgaaggac gtggtcgacg agctcgagcg
     2281 cgatccatcg cttctgtgca agggcccggc gtggctcgcg cacgcgatcc tcgacaatgc
     2341 ggtcgatcgg tatctgccga tcgtcgatca gctcgacgag gatatcgaga agctggagat
     2401 ggccgcgcta ttgaaggcgg ggacgccgaa agggcccgcg gtgctgaccc gcatcctgca
     2461 ataccgccgg ctccttcagg acttgcgccg gatgagcatc caccagcgcg agatcttgct
     2521 gcggctcgca cggggcgagt tcgacgagat tccgcgcgag gtcgtgccgt tcttccgcga
     2581 cgtcttcgat cacttcgttc gaatcaacga cttgctcgac ggttttcgcg acctcatcac
     2641 cagcgcgctc gaggcgtacc tgggcgtgca gaacaaccgc atgggcgagg tgatgaagac
     2701 cctgacgctg atctcgacgg tgatgctgcc gctcacgttt gtcgtcgggc tgtacggcat
     2761 gaacttcaag cacatgccgg agctgaactg gctctatggc tatccattgg cgctcgcgct
     2821 catgggaacg atcgccttcg gcatcttgtg gtggttccgc cgcaagggct ggatcggcag
     2881 caccgacctt cccgaagagc gtccgcgcgg cacgactcgc aaagcgaagt cggttgtcgc
     2941 aaagccagca accgcaaagc cagctccggc aaagtcagct gccgcgaagc cggcggctaa
     3001 atcggagcca agctcagatt gacgcgcacg cgtgcgttgc tgccggtgag cagcaccgtc
     3061 acgtccttgc gccagccggt ctcgaacgcc gcgctcacgg tctcgtcgcc aagcgagtcg
     3121 ttgaacagcg gatcgtgctc ggtgaggttc gcgcgcaggc ggatcgattg tcccgcttgc
     3181 ggcttcacct cgatcactgc ttcgccaagc ggctggctcg cattgccgat cgatttgcct
     3241 tcctggatcg tgacgcggtt gccttcactc ttgttgaaca gcgtcgctgt ggtcgtaccg
     3301 gtcgcgttga cgaggccgta gatctcgagc gcgccgccgc catcaccgcc gtcgtcgtcg
     3361 accgcgatcg aattcagcgt gaccttgatc ttctgcgcga cgcgcacgca gtccttgacc
     3421 tcgtagtccg tcgtgaacga catccgcgcg ggcgagttgt ccttcaggta gctcagcttg
     3481 tacgcgatcg gggcgccggg ggattcgcgc gtgtagttgc cgccggtctt gatgaagtcg
     3541 atcagcgagt cgtagctgtc gatcgtcttg accgccgtac cggcatcgcc gccgagtatg
     3601 aacgcggtga tcttgctgct cgacaggatg tccttgtacg tgaccgacac gtcgcctttg
     3661 acctcgacac cacccgagta cgcgaagtcg agcgcggcac cgagctcgga cgcgctgtac
     3721 tgcgactcga acgtgaacac gatcatgcgg ccgtacgtca ccgacgacac gtaggccggc
     3781 ggatgctcct cgttcatctt cgccttgacg tcctcgagcg acaccgaatt cgcgaacagc
     3841 gcgctcggcg aggccggcgc gtccaggtcg acggtgtagt acgcctgcgt gtagcgcacg
     3901 acgtaccgcg aacggacgtt ctggctctgc cagtcaaagc tcgtcttcag gctcgcgagc
     3961 ccgagtgccc agctcgcctg gacgccgagc gccatgttga gctgctgttc ggagtgcacc
     4021 tcttcgatct cggagtagat gtttgcgggc gtcgagccgg tgatctccga ctggagcacg
     4081 ctagccagcg catcgcgata cgccgagagg ctcggctcgg cgatcgtcgc cttcttggtg
     4141 ccgccgaggt tctcgaggct caccgagaac gtcgcgggcg cgcggggcaa caccacctgc
     4201 gtgaacaagc cgctgacgac cgagtcggcg ctgacgatcg cgcctggcca cagcgagtcc
     4261 gagttcgcgg cgtaggcgac gatgcgatcg agctgccgcg tctctttgag gttctcggtc
     4321 gtgcacgagt agtcgccatc acgcgcggcc gcgctctgac cgccgggcac tacctgcggc
     4381 ggatcgaccg gcaggtacgg cagcgactgg atgtacatgt cgatcgcatt ggggtcggcg
     4441 ctggtggcgc aggctgaaac caacgtcaga atcgcgagcc agttagagga gatgcgcatg
     4501 ggtggctagc ggccaagcaa gccgactgcc agatcgcggc aatggtccga ccacgactgt
     4561 ttgcgctcca agtgcatggc agccccgcgc ttgccgaccg ctgaatgcac agtgcggggt
     4621 cggcccccat ggcaacaaga gttgcccggt cactgcggtg gccgcacaat ttttggctca
     4681 tgcgggtgct gtgctcggcg attgtcattc tctgcatcag cctcactagc gcgcacgccc
     4741 gaacggcgac cggttaccgg cacgggcaaa agacccggat caagctggtc gaggtcggcg
     4801 gcgtcgagct cgaagcggca accgccacgg cgttccgggc gatggcgaag gcggccgcca
     4861 agaaggggat cgagctcgcg atccgcagcg gcttccggag tcacgagaag cagaaggagc
     4921 tgtaccgcga ctaccggcgc ggctggggcc acctcgctgc tcgaccgggc tactcgaacc
     4981 atcagagcgg tcgcgcgctc gacatctaca tcgacgacta ccgcgtgtac gagtggctca
     5041 aagaacatgc gcgcgactac ggcttccgtc gcacggtccg ccgcgaggcc tggcactggg
     5101 agtacgtcgg aggcgaacgc gccgctcgac gcgcgaccag ttagcggtaa ggtctgagta
     5161 tgtcgatcac gatccgttac tgcagcgctt gaggctatcg atccaaggct gcccgggtgg
     5221 cagccgaagt gaagaagagc accgggctcg ataccgagct gaaggttggc aactccgggg
     5281 agttcaccgt ctggatgaac gaccgcgtcg tcgccgagaa gaagctcgga cggtttccgt
     5341 cgcccgagga atgcgtagcg gcgatcaagg ccgctctgcc tccgagctag cgcaagaacg
     5401 cttgtagatc gaagcccggt tcttcgacgc ggtcttgcat gcccgcggcg gcgcgaaacg
     5461 atcggcacgc gccgcacgcg aggcagagcc ggatgtcgcg gccgccgagc ttgccgccga
     5521 tgccatagat ccgcacggcg acttccttac agctcggaca ctcgtggtga tagacgtcct
     5581 gctgcttgaa gccgcgcttg gcgagcttgt attcgatcgt ggcgaggtcc tcgacgggca
     5641 tcgccggaag tatcgcacgc taatcgcggt agctgcggtc gaggcggtcg agcttgcgga
     5701 gcaggccggg ccacgcgagt ccgccaccgg ccgagcgggt gacttgcttc gccatcatct
     5761 gcgcgccggc cacgatcttc tggtcgatcg ggatcagctc gctcgacgcc gcctgcgcgc
     5821 gcagctggat cgtgcacgcg ctctcgaaga tgtacatcag caggaacgca tccgggatcg
     5881 acgggccgac ggtgagcagg ccgtggttgc gcaacatgaa gaagctcttc tcgccgaggt
     5941 cacgcacgag gcgcggcttc tcgccctcga gcagcgcgac gccttcgtag tcgtgatacg
     6001 cgagcgacga cagcacgaag atcgattgct gcgagaccgg caagacgccg cccttcgtcg
     6061 cggacaccgc gacgccgttg atgctgtgcg tgtgcaggac gcacttcgcg tcttcgcgcg
     6121 cggcatggat cgcgctgtgg atcgtgaagc cggcgggatt gatctcgtac gggctgtcca
     6181 tcagcttctt gccctcgaga tcgatcttca cgaggctcga tgcggtgacc tcgtcgaacg
     6241 tcatcccgta cgggttgatc aggaactggt ggccctcgcc ggggatgcgc gccgagatgt
     6301 gcgtgaagat caggtcgtcc cagccgtaga gcgcgacgag tcgatagcac gcagccagat
     6361 cgacacgcag gttccactcg gcttcgctga ccttggactt cacatcgatc atgcgccgat
     6421 gctacgggaa ggcatgggcc gtggtgaaga cgtcgcgaag gcgatcgctg cagatctcca
     6481 aggcaagagc cagccgggcc gggccaagaa cgagaaggcg tatctaaagt cagacctcga
     6541 ccacgtcggc gtgccgatgc cagcgattcg ctcgacggtt gttgccgcgc tgcgtgatgc
     6601 aaaggatctc acccgcgcgg agttgctcgc ggccatcgcc gcgttgtggg atggcgtgca
     6661 cgagcaccgg atggcggcga tcgagctact cgtcaagcga cagaaactgc tgacgccccg
     6721 cgatgcgaag ctcgtcgaga agctgctacg cgattcgagg acctgggcgt acgtcgacgt
     6781 gctcgcgacc cacggcatgg gcccgctcgt cgtcgccgat ccgtcgctcg agcgcacgct
     6841 cgaccgctgg gcaaaggacg acaacttctg gatgcgccgc tcggcgatgc tcgcgctgct
     6901 cggcccgctg cgtaccggcg gtggcgactt cgcgcggttc ggccgctacg ccgacgcgat
     6961 gctcgacgag aaggagttct tcatccgcaa agcgatcggc tggattctgc gcgagacgag
     7021 ccgcaagcag ccgaagctgg tgttcgactg gctgctgccg cgtgcggcgc gcgcgtcggg
     7081 tgtcacgatg cgagaagccg tgaagtatct gaccccagcg cagcgcgctc gcctcggtcg
     7141 ttagcgcagc gccgcggcgt gatcgacgat gacgtggggc gccccacgct cgagcgcctt
     7201 gcgatagtcg tcggcgaccg cggtcttggc gccgtgacgg tcgaacgcga cgtagacagt
     7261 ctcgacggtc gcgagcagcg gaccattgaa gcggtggagc tcggtggcga tcgtgaacga
     7321 cgtcgtgccg acgcgaatcg tggacacgcg acagtcgagg atgtcgtcgt aacgtgcaga
     7381 cgccttccac tcgacggtct gcttgacgag cttccagtcg atgccggtga cggctggatc
     7441 gaccgaacca aacagcgcgc gcgagtattc gccgaccgca atatcgacgt agtcggccca
     7501 ccgcgcgttg aacacgatgc cttgcgcatc gcactcgcta tagcggacgc gcaagagata
     7561 gcggaacggg gtcaccccgc gacgctatac cgcgcggcga ttgaacaggt gcaggatgat
     7621 caacagaacg acggcgccga tgaaggcgac gaatatcgtt ccggccaaac cgccccacgg
     7681 agccgaccaa ccagccgcac ggaagatgaa gccgccgatg aacgcgccga cgataccgat
     7741 gacgatatcg gcgagcaggc cgtacccacc gacgatgaga ccggcaagta caccagcgat
     7801 caaaccaaca atgagccagg tgagaatatc catgctcctg ggtagagcat tgcgcatgcc
     7861 acgggtgcgt cctcgtggtg ccgcgcgtcg gcgcctgcgc atcggaggac gtccgagtgc
     7921 gcgcgagcac ggcgctcagc gggcttcgcg cagtaggcgc tcggcattct ctttcgagat
     7981 caccgcgggc ggatcgacga cccgcttcaa gagctcgacg tagaccggat cactcttcag
     8041 cgcgcggcgg acgagcggca aaccggcgtc gtattggccg ccaccggcga gcgagacacc
     8101 ggcccagaac gccatctcgg tgttgttggg ctccatgcgt gtcgcggtcg tgtagtgctc
     8161 ggcggcgccg gcgatgtcgt tcttctcgag cgcagcatcg cccttgttca tgtggtcgta
     8221 ggcgcgcgcg agcgtgacga ggcggtgcag ctctttgagc ggctcggcgg cgtcctcgac
     8281 gcgcagatcg agcttcttct cggcccacgg cttgtcggtc ttgttgccgc tgacgatcag
     8341 gatcgccgcg gattggcatc cgcgcaggtc gccgcccgta gcctgcgcgg cgtcgagtgc
     8401 cgcgagcatg cgatcggcga gatcgccctt cgtcgtctcg aacgcctgcg ccatcgcggg
     8461 cacgaccttg tcgttcgcca tgatgtttgc ctgcaccgtg taaccatcgc cggtcttgct
     8521 gctcgcaaac gcgatgcact tcgcgccggt gtgcgacgcg acgcggcctt gcgcatcgac
     8581 gaagccgagc tggcgcgtcg cgctttgtgg atcggcggca gtgagctgcg cgatcgcgtc
     8641 ggcgggtgca acaccatcgc gcatgagcgc gaggcccttc ggcccgtagc ccggttcggc
     8701 gaacgcttgg gtcgcgaccg cgccgacgcc gggctccgcc cacgtgacag cgctgccgac
     8761 cgagaaccag tgagactgca ccgcgacgcc gagatcgccg gtcactggat cgcgggcgac
     8821 gatcgaaaac gtattcgtcg gccgtaccga cgaaccgccg agcgtgccga gcggcgtagg
     8881 ggcagctacg tagtgactct cgccgcggca gtaatcaccg ctcggcgtgg cagatttcgt
     8941 cgagccttta caggcggtca acgcgatcaa gagtaggagg gctcgcatgc cgccgactgt
     9001 atgggaaact gccggcgtga ggtgcctcgt cgtgctcgct ctcgctgcgt gtggaggcac
     9061 gccgaagcct gcatcgaccg aatcgggccg gccgccgtac ctggcgctgc tgcagcacgg
     9121 tcaacggtgg tcgctgccga tcgagaccgt caatggcgag caccgcggtg atgcgtgggc
     9181 ggcgaccagc accgagcgcg gcgcgctcac gtgcgaggtc gctgacgtga agacgatcgc
     9241 cgacgccacg atgtcgcacg tgcagtgcgc gccgccgtac gccgggctac tcgtcgtcgg
     9301 ctactgggtc gcgacgccgg caggtctcta tcaccccgcg cttcccgtcg atgacgtcga
     9361 cgagctcacg ctactcggcg agaacgatct gctgctgacc gcgaagccga aggagcgcga
     9421 gcacagtcag gcgaccgccg gtatgcagca cgcgatcgag gcgtttcgct tcaagcagag
     9481 ctggtgcatg cgcgatgcgg tggtcaccga agaagatcgc cgccacttca cgctctgcgt
     9541 cgatggatcg ggcatgaccg gtggcggcga gctggttgtc agcggcaccg accacagctg
     9601 gcaccgctcg acgttcggtg tggtcgccga cgatcccgac gatccgacga ccggtcacga
     9661 cgactgatcg tgctcggcga tgatccgctc ggcttgctcg atgtcgtggc ggcgcgcggc
     9721 gcggcgatag atcaccgcga gtacgacgag cgctgcgacg ccgaacgccg acgcaaccac
     9781 ccagacgacg tggagatagt gatgatcgtg gccgccgagc atcgctagtt cccgaacatc
     9841 gtgtcgaagc cgccattgaa ccgggtgacc gcgctgatga tcggcgacgc gactgcgacg
     9901 tactgcagga gcgctgcgac gaacagcgtc acgaggacgc cgacggcgag tagcgacgcc
     9961 gcgcgaccgc tggtctcgct gccggtctgc gcgagcacgc gccggcggac cgctcgcccg
    10021 aacagcagcg ttgcggttgc gagcgcgatg aacaccgagc ccagcgcgat cggcacgccc
    10081 gctttcgagc tcgtgtggta gtagatcgcg acgagcggcg cgagcgtcgc gagcacgagc
    10141 gtgccgccga agatcgccag cgcgaacgcg ctcgccagct cgacggccgg cagcttcgca
    10201 ttcgtcagtc gcagcgcgac gagtccggcg ggcagcgcgg tcagcgccga caacagaatc
    10261 accatcggca ccttgtacgc attcgcggcc gcgagcgcgg gtgacgcgct acccgccgcg
    10321 actccccaca gcgacgacag cgcgagtgcc gcgacgagcg cgagtaccgc gagcatcgtt
    10381 tcctgcgcgc gcgtgaccgg gcgggcgagg tgcccaccgc aggtcagcgt catgagctga
    10441 gacagacggt ttgtttccat gccgacctct ggtagcaaca ggcgtgcaat ccacctcctg
    10501 ctcgcgcttg ccgtgcgctg ccgccgtctg cttgctcgtg ctgtggtccg catgcgccac
    10561 gtcacataga ttcacagcag acgacgatgc ggcggtccgg cacgtgctca ccgcgcaaca
    10621 ggaggcgtgg aatcgcggcg acctcgaagc gtacatgagt ggctacctga attcgcccga
    10681 gctcgtgttc acgtcggcga gcaagatccg gcgcggctgg caggagacgt acgacaagta
    10741 caagacgaag tacggctccg acaaagcgac gatggggaag ctcgcgttcg agatcctcgg
    10801 cgtgcaacag ctcggcgccg acggcgcgat cgtgctcggc cgctggacgc tcaccgagac
    10861 gccgaatgcc ggctctggcg tgttttccgt cgcgttgcgc cgcaccgagc gcggctggct
    10921 cgtcgttcac gatcacacgt cgagcgatta gcgctgtcac atcggtcgcg agccggcact
    10981 acagagcatg aagccgaacg ctctgctctt gtttccacta cttgccgcat gcaccgacct
    11041 cgacatggtc gagatcaccg agcaggacgt cgttctgcct gcgacgaaag tctgcgcgca
    11101 ggacgtggtg ttgcacccga cgagcgatta tgtggcgctc gcgtcgctgc atcaggactt
    11161 tccgaacggt ggggctcggc tgatcagcgc gaccatgctc gggacggtga cctcgagctc
    11221 gggcagcgcg tcgccgatgc agggtcttcg catctcgtgc gtcgggcccg agaaccgaat
    11281 gatccagacg accgagaacg cgatcaaagg cggtggctcg ctggcaacga aagcatcgct
    11341 cacgatcacc ggccccggca gctgggactg cacgctcggt ggcatcacga tgcacactgg
    11401 cgcggcactc gcgatcaccg gcagcgagac atgcctgtcg tcggccgtca gcgatgcgca
    11461 ggactggccg cactggccgg cggatagcgc gaacgtcggc gacggccagc gcgtccgcgc
    11521 gggctactcc acatacgtgc tcaaacatga ctacgcgctc gcgtcggacg tcgcgacgtt
    11581 cggtgttcgc ggcaacatcg agctcaccga catcaccggc gacgcatcag gcaccaatat
    11641 cgccgagtgc atggactcca ccgccgatcc gaccgcacct gcgtacccgc gcctctacat
    11701 ctcggcgcaa cagaagagca cgacgggcta ctgcggctcg cgcgtcgtcg ggccctacct
    11761 caatacgacg atcagcgcca agtcacacca caagaaggtg tatccggtgc tcgataacgt
    11821 gccggtgtca aacgcgctcg gctgcttgcc gacgttcgag gtcaaggtga tggtcagtgc
    11881 gctcgccggc ggcaacgcgc tctgcttcca cgcggcacgc tacagcaacg tcgttgtcgt
    11941 gccgcagagc tacgacggtc cggtcaacgc gctgaactag acgccgctcg gcacgagcga
    12001 cggcatcatg cgcgcgacct cggtcggcgt gatgccgagc cggcgcgcgt agtcggtgac
    12061 ctggtcggct tgcatgcggc cgaccgcgaa gtagcgcgcg tcgggatgcg cgaagtagag
    12121 accggacacc gatgccgtcg gcaacatcgc gtagctctcg gtcagcgtca cgccatggtg
    12181 ctgcgcgttg tcgagcaacg cgaacagcgt gcccttcggc gtgtgatcgg ggcaggcggg
    12241 atagccgaac gcgggacgga tgccgcggta cttctccgcg atgatgtcgt cgtgcgtgag
    12301 gccctcgctc gcgccatagc cccactcgac gcgcgcgcgg tgatgcatgt gctcggcggc
    12361 ggcctcggcg aggcggtctg cgagcgcctt ggtcatgatc gcggagtagt cgtcgtgctg
    12421 cgcttcgaac gagccggcga gctcgtcgac gccgaggccc gcggtgacga tgaacgcacc
    12481 gacgtagtct ttgcccggag tcggcgccac gaagtcggcg agggacaggc acacgtcctt
    12541 gtcttcttgc tggcgcagca tcgggaagcg cttgtcgccg acgacgaggt cgtcgccctc
    12601 ggcgtgcgcg gggaagaagc cgtaggtcgc gcgtgcgcgc aacagcttgc cgccgacaat
    12661 gcgctggagt agggcctggc cgtccgcaaa taccttgcga gcagcgtcgc ccttcttcgg
    12721 gtcgtcgaga atcgccgggt agcgtcccga cagctcccac gtgtggaaga acggcgacca
    12781 gtcgatccac ttcgcgagct cggcgagatc gaggtcgacg ttcttgatgc cggtgaacgc
    12841 gggcttcgcc acggtcgtga agtccggctt catccggcgc gcgcgcgcgt cttcgagcga
    12901 gagcagcggc cgcttctgcg tagcgacgaa tgcctcgcgc tgcgacgcct gcttcgctgc
    12961 gacctcggcg aggtagttct cgcgctcgcc cgacatcagc ttgccgagca cgccgaccgc
    13021 gagcgatgcg tcgggcacgt gaacggtcga gccggcatac gcgggcgcga tcttgaccgc
    13081 ggtgtgcttg ccgctcgttg tcgcgccgcc gatgagcagc ggcaccttca tgttgcgccg
    13141 ggtcatctcg gatgcgacgc cgatcatctc gtccagcgac ggcgtgatca ggcccgagag
    13201 accgacgagg tccgcgtcga gctccgctgc ggtgtcgagg atcttgtgcg ctggcaccat
    13261 gacgccgagg tcggtgacct cgtagccgtt gcaacgcagg acgacgccga cgatgttctt
    13321 gccgatgtcg tggacgtcgc ctttgacggt ggcgatcacc atcttgccct tcgccgcgcg
    13381 catgccggtg cgctgcttct ccgcctccat gagtggctcg aggatcgcga cggccttctt
    13441 catcacgcgc gcgctcttca cgacctgcgg caggaacatc ttacccgcgc cgaagagatc
    13501 acccaccacg cccatgccat ccatgagcgg gccttcgatg accttgagcg gcaccttgag
    13561 cttgtcgagc gcttcctgag tgtcttcgtc aatgaagtcc gtgatgccac gcagcaaggc
    13621 gtgctcgaga cgcttctcga ccggcgcttc ccgccacgcg aggtcgacgt cctgtttttt
    13681 cgcgccggtc tgtcctttga actgttccgc gtagtcgacg aggatctccg tggcgtccgg
    13741 gcggcggttg agcagcacgt cctcgacctt cacgagcatg tcctgcggga catcctcgta
    13801 cacttcgagc atgcccgcgt tcacgatgcc catgtccatg cccgccttga tggcgtggaa
    13861 caggaacgcg ctgtgcatcg cttcgcgcac cttgttgttg ccgcggaagc tgaaactgat
    13921 gttgctgacg ccgccgctga ccttcgcgcc gggcaggttc tgcttgatcc agcgcgtggc
    13981 gttgatgaag tcgagcgcgt agttgttgtg ttcctccatc ccggtggcga cggtcaggat
    14041 gttcggatcg aagatgatgt cctgcgg
//
LOCUS       DASUFX010000803         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_548054,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000803 DASUFX010000000
VERSION     DASUFX010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548054"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFV99_32505"
     CDS             <1..817
                     /locus_tag="VFV99_32505"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5064158.1"
                     /translation="FERRPKDLYFGIGNNGDTPEARYREQLARGAATLDMKRTDAFHV
                     RVAGAFTNLDFDDSKEGPPIQMLYPPSMLTGFTGTRNLYAELELRYDTRRITNTLEQM
                     GVLADAFGGRIYQLEAGSDYWRYGGEAIHFQPLGIGRTLATRLHFESVSGSYNDVAFN
                     QLPELGGKMLLRGYAVDRFRDRVAIASSVEYMWDISSFVLASLFVDAGRVYPALDELT
                     FDDMRLGYGASLQLVDRRRFLAGVSVASSIDGGVFLNLVLDPIYEPEARVKQK"
     gene            814..2463
                     /locus_tag="VFV99_32510"
     CDS             814..2463
                     /locus_tag="VFV99_32510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064159.1"
                     /translation="MRLPLLLLVVVACSGAPPVPHVRYANAPAIEVVNDRRDVRKKPV
                     EVPYERYLENFDGSFHRLMTRKLELKRPERARGVNALDEVPDSTWFTNRIGVREVSVA
                     EIATVPGSIGTPENYKPWTIISSKVGGLTVGFIMKDTRGEKFLLKFDQRSFPEAETAA
                     QIVMGRLLWAIGYNVTDDYIVHLRREDLVLAPDAVDKNPEGVEVKLDDHRLQARLATI
                     NINKDGSMRALASRYLEGKPLGGHPPEGVRSDDPNDRIPHELRRDLRGTQAIFAWLDH
                     SDVHTGNTLDMWVSDPENPSWHYVKHYFIDFGIALGFGAAKNKNPRYGYEYEVDWSAM
                     ARSLFSLGLVQRPWEDRKRPQLRGIGTYDIARYDPGTWKPLTPMYTPIRVADRIDNFW
                     SSKIFMKIRRQHIEAAVDAAQLSDPRARAWLVEALIARQRKTAKYWFERVNPVDEIHV
                     VDGGLCFKDLSLTYAFEPIATTRYTVTSYNRRGKRVVATASFAARGDVSCAPLALADD
                     VDSYTIVRVVTRRPNFKRATFVYVARALPTAPPRVIGIWRQ"
     gene            2507..3985
                     /locus_tag="VFV99_32515"
     CDS             2507..3985
                     /locus_tag="VFV99_32515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5064160.1"
                     /translation="MRRNAAISNRLERWLSPFTTVHPGEGARAVLLLVCIFLIMTAFY
                     VMKTAREALILTGGTFGLHGDELKTYATGAMALLLIVLVPAYDALADRHRRIRFINLT
                     YMVLLGSLIAFFLMGEAGLPIGFAYFVWFGLMSAFMVAQFWSYATDVYTEEQGKRLFA
                     LIATGGTLGAIVGPNIAKLASTFTLMPVAATLLVFAIILFNVVEHHAPRERRQKDPIE
                     GPGGFALVVGDRSLLLIAALLLLANIVNSTGEYLLSHAVREHAMALVPGTDAVAEAQR
                     REVIKSFYSDFFLVVNTLSFLVQAFLVSRVIDKMGVRRALFILPVIAFGAYAAIGLIG
                     GLAVVRAAKTAENAADYSVQNTVWQTLFLRTDRAVKYKAKATLDTFFVRCGDALSAIV
                     VAVGIHQLHFGSRELAFVNLGLVIAWLAVAVSIAHTERFLKREPHERRSMRHTSGLIY
                     QSSSKRMRRGGVSTSSSWPDRTTQTNASTAPPAINSASGSTT"
     gene            complement(3993..>4758)
                     /locus_tag="VFV99_32520"
     CDS             complement(3993..>4758)
                     /locus_tag="VFV99_32520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5064161.1"
                     /translation="GATATDMQEELGQGVRGTVRGHRVLVGSPAWVRTQCPRVRDEIE
                     RWINDVTREAQTPIAIAVDGAIVAIAGIADGIRNDARVAMRALEHLGWRVEILSGDDA
                     RVVEAVGRELELPAERCIGEVAPEIKLAIVEAEREQGPVAMVGDGVNDAAAIAAATCG
                     IAVSGAAEVAIDAADVYLREPSIAGIAATAAGATATLVTIRRSLKLSLAYNLIAGSLA
                     IAGVIHPLIAALLMPASSLTVLANSLRSRAFRKENR"
BASE COUNT          839 a         1555 c         1504 g          860 t
ORIGIN      
        1 gttcgagcgc aggccgaaag atctgtactt cggcatcggc aacaacggtg acacccccga
       61 ggcacgctac cgcgaacaac tcgcgcgtgg cgcggcaacg ctcgacatga agcgtaccga
      121 cgcgttccac gtccgggttg ccggcgcatt taccaacctc gacttcgacg attcgaagga
      181 gggcccaccg atccagatgc tctatccgcc gagcatgctg acgggcttta ccgggacgcg
      241 gaacctgtat gccgagctcg agctgcgcta cgacacgcgt cggatcacga acacgctcga
      301 gcagatgggt gtgttggccg atgcgtttgg cggccgcatc tatcagctcg aggcaggcag
      361 cgactactgg cgctacggcg gcgaagcgat ccacttccaa ccactcggta ttggccgcac
      421 gcttgccacg cggctccatt tcgaaagcgt gtccggctcc tataacgacg tcgcgttcaa
      481 tcagcttccc gagcttggcg gcaagatgct gctgcgtggg tatgcggtag accgattccg
      541 tgaccgcgtc gcgatcgcga gctccgtcga gtacatgtgg gacatcagct cgttcgtgct
      601 cgcgagcctg ttcgtcgatg ccggacgcgt ctacccggcg ttggacgagc tgacgttcga
      661 tgacatgcgg ctcgggtacg gcgcgtcgct acagctcgtc gatcgccgcc gctttcttgc
      721 gggcgtcagt gtcgcatcgt cgatcgatgg cggggtgttt ctaaacctgg tgctcgatcc
      781 gatctacgag cccgaagcgc gggtgaagca gaagtgaggc tcccgctcct cctccttgtt
      841 gtcgtcgcgt gctcgggcgc accgcctgtg ccgcacgtcc ggtacgccaa tgcgccggca
      901 atcgaagttg tcaacgaccg ccgcgatgtg cgcaagaagc ccgtggaggt tccgtacgag
      961 cgctacctcg agaacttcga cggatcgttc catcgtctga tgacccggaa gctcgagctc
     1021 aaacgtcccg agcgcgcgcg cggtgtgaac gcgctcgatg aagttcccga ttcgacctgg
     1081 ttcacgaatc ggatcggcgt acgcgaggtc tcggttgccg agatcgccac ggtgcccggc
     1141 agcatcggca caccggagaa ttacaagccg tggacgatca tcagcagcaa ggttggtggc
     1201 ttgacggtcg gcttcatcat gaaggacacg cgcggtgaaa agttcctgct caagttcgat
     1261 cagcgcagct ttcccgaagc cgagaccgcc gcgcagatcg tgatgggccg gctcctctgg
     1321 gccatcggct acaacgtcac cgacgactac atcgttcacc tccgtcgcga ggatctcgtg
     1381 ctggcgcccg atgcggtcga caagaatcca gaaggcgtcg aggtcaagct cgacgaccac
     1441 cgcctccaag cgcgattggc gactatcaat atcaacaagg acggctcgat gcgtgcgctc
     1501 gcctcgcgtt atctcgaagg caagcccctc ggtgggcacc cgccggaagg cgtgcgaagc
     1561 gacgatccga acgatcgcat cccccacgag ctacggcgcg acttgcgcgg cacgcaggcg
     1621 atcttcgcgt ggctcgacca tagcgacgtg cacaccggca acacgctgga catgtgggtc
     1681 agcgatccgg agaacccgag ctggcactac gtgaagcact acttcatcga cttcggcatc
     1741 gcgctcggct tcggtgccgc aaagaacaag aacccgcggt acggctacga gtacgaggtc
     1801 gactggtccg cgatggcgag gtcgctattc tcactcgggc tcgtgcaacg gccatgggag
     1861 gaccgcaagc ggcctcagct gcgcggcatc ggcacctacg acattgccag atatgatcct
     1921 ggtacatgga agccgctgac gccgatgtac acgccgatac gagtcgcaga tcgcatcgac
     1981 aacttctggt cgtccaagat cttcatgaag attcgccgtc agcacatcga ggcggcagtc
     2041 gacgccgcac agctaagcga cccgcgcgca cgggcctggc ttgtcgaagc gctgatcgcg
     2101 cgccagcgca agacggcgaa gtactggttc gagcgggtca accccgtcga cgagattcac
     2161 gtcgtcgacg gtggcctctg cttcaaggat ctctcgctca cctacgcatt cgagccgatc
     2221 gcgaccacgc gttacaccgt cacgtcgtac aaccgccgag gcaagcgcgt ggtcgcaacg
     2281 gcgtcgtttg cggcccgcgg tgacgtgagc tgtgcgccgc tcgcgctcgc ggacgacgtc
     2341 gacagctaca cgatcgtgcg cgtggtcacg aggcgtccga acttcaagcg cgcgacgttt
     2401 gtctatgtcg cgcgtgcgct cccgactgcc ccaccgcgcg tgatcggaat ctggcggcag
     2461 taatcgtgcg tagagcgcgg cacctgcatt gcagctatcc gctgtagtgc gacgaaatgc
     2521 agccatatcg aaccgtctcg aacgttggct aagccctttc acgaccgttc accccggcga
     2581 aggcgcgcgg gcggttctgc tgctcgtctg catatttctg atcatgaccg cgttctacgt
     2641 catgaagacg gcgcgcgaag ccctgatcct caccggcggc acgttcggcc tccacggcga
     2701 cgagctcaag acgtacgcga ccggggcgat ggcgctcctc ttgatcgtcc tcgttccggc
     2761 ctacgacgcg ctcgccgacc gtcaccgtcg gatccggttc atcaacttga cgtacatggt
     2821 tctgctcggg tcgctgatcg cgttctttct gatgggcgaa gcgggactgc cgatcggctt
     2881 cgcgtacttc gtgtggttcg gcctgatgag cgccttcatg gtggcgcagt tctggtcgta
     2941 cgcgaccgac gtctacaccg aggagcaggg caaacggctg ttcgcgttga tcgcgaccgg
     3001 cggcacgctc ggcgcgatcg tcggtccgaa catcgcgaag ctcgcgagta cgttcacgtt
     3061 gatgccggtc gcggcgacct tgctcgtgtt tgcgatcatc ttgttcaatg tcgtcgagca
     3121 ccacgcacca cgcgaacggc gacaaaagga tccgatcgaa ggcccgggtg gcttcgcgct
     3181 cgtcgtcggg gatcgcagcc tgcttctgat cgcagcgctg ctgctactcg cgaacatcgt
     3241 caactcgacg ggcgagtact tgctgtcgca tgcggtgcgc gagcacgcga tggcgctcgt
     3301 gccgggtaca gacgccgtcg ccgaagcgca gcgccgcgag gtcatcaagt cgttctacag
     3361 cgacttcttc ttggtcgtga acacactcag cttcctggta caggcgttcc tggtgtcgcg
     3421 cgtgatcgac aagatgggcg tccggcgtgc actgttcatc ctcccggtca tcgcattcgg
     3481 tgcgtatgcg gcgatcgggt tgatcggcgg gctcgcagtc gtgcgagcag cgaagactgc
     3541 cgagaacgcg gccgactatt cggtccagaa cacggtctgg cagacgctgt tcctgcgtac
     3601 cgatcgcgcc gtcaagtaca aggcaaaggc gacgctggac acgttcttcg tgcggtgcgg
     3661 cgacgccctg tctgcaatcg tggtcgcggt cggcatccat cagctgcact tcggttcgcg
     3721 cgagctagcg ttcgtcaacc tcggtctcgt catcgcgtgg ctcgcggtgg ccgtgtcgat
     3781 cgcccacacc gaacgcttcc tgaagcgcga gccccacgag cgccgctcga tgcggcacac
     3841 gagcggcctc atctatcagt cgtcgtcgaa gagaatgcgc cgcggcggtg tatcgacatc
     3901 gtcgagctgg cccgaccgca cgacccagac gaatgcgagc acggcgccgc cggcgatcaa
     3961 tagcgcgagt ggcagtacga catagagcgc ggtcatcggt tctccttgcg gaacgcgcgc
     4021 gagcgcagcg aattggcgag cacggtgagt gagctcgccg gcatcagtag tgcggcgatc
     4081 aacggatgga tcacaccggc gatcgcaagc gagcccgcga tgaggttgta cgcgagcgac
     4141 agcttcaggc tgcgccggat cgtgacgagc gtcgcggtcg cacccgctgc cgtcgcggcg
     4201 atgccagcga tcgacggctc acgcagatac acgtcggccg cgtcgatcgc gacctcggcc
     4261 gcacccgaca cggcgatacc gcaggtagcg gcagcgatcg cagcggcatc gttgacaccg
     4321 tcgccgacca tcgcgaccgg gccctgctcg cgctcggcct cgacgatcgc gagcttgatc
     4381 tcgggcgcga cctcgccgat gcagcgctcc gcgggcagct cgagctcgcg accgaccgct
     4441 tcgacgaccc gggcgtcgtc gccggacagg atctcgactc gccagccgag gtgctcgagc
     4501 gcacgcatcg cgacgcgcgc atcgttgcgg atgccatccg cgatacctgc aatcgcgacg
     4561 atcgcgccgt cgacagcgat cgcgatcggt gtttgcgcct cgcgcgtcac gtcgttgatc
     4621 caacgctcga tctcgtcgcg cacgcgcggg cattgagtgc gcacccacgc gggcgatccg
     4681 acgagcacgc gatggccgcg cacggtgcca cgcactccct gcccgagctc ctcttgcata
     4741 tcggttgcgg tggcgccc
//
LOCUS       DASUFX010000804         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1518692, whole genome shotgun sequence.
ACCESSION   DASUFX010000804 DASUFX010000000
VERSION     DASUFX010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518692"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VFV99_32525"
     CDS             complement(<1..1565)
                     /locus_tag="VFV99_32525"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX5064162.1"
                     /translation="MPDARQLAKRLRDAAPVPVVDVQRSMTLQPEHIYVLPKDREIAI
                     HKSWLAIDQPDGHGSRDRLLRSLADSLGPDCAAVILAGRGSDGVLGAKRVKEAGGITI
                     CQAGVEDEANELPRIASATGIIDLVLPVPEIGPRLVSLTRESEADGPEEPFGAAGTGD
                     GEAFAEILMMLYARTGNDFNAYRRGTLFRRLLRRMLIAEVGSIDAYSRYLRDHPTELD
                     RLFRELLVSVTDFFRDPQAFELLANDIVPRLFKGKGAGDQIRVWVPGCATGEEAYSLA
                     MLLNEHASRTRNAPTIQLFATDIDEEALREARVGIYPDAIATDVSEERLQRFFTHEQD
                     HYRVSPALRGITEFSAQNVLRDPPFSRLDLVASRGLLLELNDDAQEHALETFHFALRP
                     DGLLLLGPGERARDSHFTPIDEKHHIYQRRTSPFRRVSPRTGTSSVAVAATPSDPGEL
                     HDHALAHYALASVLIDGTLDVLHTNAPARAYFKYSAGELTRSILRLVHPALSPALGRA
                     IYAAREPGRGTVIR"
     gene            1884..2876
                     /locus_tag="VFV99_32530"
     CDS             1884..2876
                     /locus_tag="VFV99_32530"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5064163.1"
                     /translation="MRIAVFDTRRHDRMALEEASSAHRHELTFLEPRLTAQTATLAEG
                     FDVACSFVNDHVDRAALTLLSDGGVRLIALRSAGYNHVDLEAATELGLTVVRVPEYSP
                     YAVAEHAVALVLTLDRKIHRAYARVREGNFSLDGLLGFDLHDKTVGIVGTGRIGAAAA
                     RIFAGFGCRVLAYDLRPQSELPVEYVPLDELYARSDIISLHVPLTPATHHMIDAAALA
                     RMQPGIMLINTSRGALIDSRALISALKTCHIGSAGLDVYEEEEGVFFEDLSDKVLQDD
                     VLARLLTFPNVVITSHQGFFTREALAAIASTTLANVDAFERGEPLVNEVRAQRR"
     gene            complement(2877..3380)
                     /locus_tag="VFV99_32535"
     CDS             complement(2877..3380)
                     /locus_tag="VFV99_32535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064164.1"
                     /translation="MSSSSRERAASGRRAVERKPVAASSSGGASTTTAGALAAATWLR
                     TRGMSSPTRWHVEIALDVMNDRAPTDFDETRATRFHLNIYTEEWGVHFVHRGRSSWIR
                     VTDIAFVHGRDEYKLLGILPALKDVGRLLRQLEQTHEIKFQRQHAAVHTNMPQAERAI
                     REWIASL"
     gene            3616..>4349
                     /locus_tag="VFV99_32540"
     CDS             3616..>4349
                     /locus_tag="VFV99_32540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064165.1"
                     /translation="MGSHKILVVDDTPANLVAAEAALAPLERPIVMAQSGEEALGHLL
                     DQDFALALLDVHMPNIDGYELARMIRSRERTEHMPIIFITAHAHDEASVLRAYDLGAV
                     DFLFRPVIPAVLRAKASVLVTLQERNVELANERVERDREQAAHQELARLNRALAEYDR
                     RKDSFIAILAHELRNPLASLRTCVDLIRAAPTQPLTPRQITALDRQTNVLMRLVEDLL
                     DVSRIKADKIELRPELVDLVGLVEAA"
BASE COUNT          774 a         1380 c         1391 g          804 t
ORIGIN      
        1 cggatcacgg tgccgcgccc aggttcgcgt gcggcgtaga tcgcgcggcc gagtgccggg
       61 ctcaacgcgg ggtggacgag gcgcaggatg ctgcgggtga gctcgccggc cgagtacttg
      121 aagtacgcgc gagccggtgc attggtgtgt agcacgtcga gcgtgccatc gatgagcaca
      181 ctcgcgagtg cgtagtgcgc gagcgcatgg tcatgaagct cgcccggatc agaaggcgtc
      241 gccgcgactg cgacggagct cgtgcccgta cgcggtgaca cgcgccggaa cggcgacgtg
      301 cggcgttgat agatgtgatg cttctcgtcg atcggcgtga agtgcgagtc tcgtgcgcgc
      361 tcgccgggac cgagcagcag cagcccgtcc gggcgcagcg cgaaatgaaa tgtttccagc
      421 gcgtgctcct gcgcgtcatc gttgagctcg agcagcagcc cgcgcgaagc gacgaggtca
      481 agacgcgaga acggcggatc gcgcagcacg ttctgtgcgg aaaattcggt gattccgcgg
      541 agcgcgggac tcacacgata gtgatcctgc tcgtgggtga agaagcgttg cagccgttcc
      601 tcgctgacgt cggtcgcgat cgcatcggga tagatcccta cgcgggcctc acgtagcgct
      661 tcttcgtcga tgtccgtggc gaagagctgg atggtcggtg cgttgcgggt gcgcgatgcg
      721 tgctcgttca gcaacatcgc gagcgagtac gcctcttctc ccgtggcgca gcccggaacc
      781 caaacgcgaa tctgatcgcc ggcgccttta cccttgaata gccgcggcac gatgtcattc
      841 gcgagtagct cgaacgcctg cggatcgcga aagaagtcgg tgacgctgac caagagctcg
      901 cgaaacagcc gatcgagctc ggtcggatga tcgcgcaggt agcggctgta tgcgtcgatc
      961 gagccgacct cggcgatgag catgcgacgc aggagccgcc gaaatagcgt gccgcgtctg
     1021 tacgcattga agtcgttccc ggtccgcgcg tagagcatca tcaggatctc ggcgaatgct
     1081 tcgccgtcac cggttcccgc agcgccgaac ggctcctcgg gtccgtcggc ttcgctctca
     1141 cgcgtcaatg agaccaagcg aggaccgatt tcggggaccg gcaggacgag atcgatgatg
     1201 ccggtcgcgc tcgcgatccg cggcagctcg ttggcttcgt cctcgacacc tgcctgacag
     1261 atcgtgatgc cacctgcttc ctttactcgc ttcgccccga gtacgccgtc ggagccgcgg
     1321 ccggcaagga tcacggcggc gcagtcgggg cctagcgaat cggccagtga tcgcagcagc
     1381 cgatcgcggc ttccgtggcc gtccggttga tcgatggcga gccagctctt gtggatcgca
     1441 atctcgcgat ccttgggcag gacatagata tgctcgggct gcagcgtcat cgatcgctga
     1501 acgtcgacaa ccggaacggg cgccgcgtcg cgcagccgtt tcgcaagctg gcgtgcatcc
     1561 ggcatcgcaa agacgagcgc aagtccgggt tgtgccggaa ggctacgtac gatctgctct
     1621 gcggccgcac catcgcgccc caccacgaca gcgactcgga gtggtgcctc cgacataaag
     1681 gcgaactgca ccacaggctt caaacgacag caagcatgtg ttcacaaaca cactgtgatg
     1741 ccgctacggg acattacgta cgtcgaaatt tcatcctcgg tgtagatgcg gtgcaatcgt
     1801 ggaatgagcg caattcttgg cgccgcatat tgttgcggta gcggtgtcga ggcagcacac
     1861 ggtgcgcgaa gtagggttcc gccatgcgca tcgccgtctt cgatacccgt cgtcacgacc
     1921 gcatggccct cgaagaggcg agctcggcgc accgccacga gctcacgttc ctcgagccac
     1981 gcctcacggc gcaaacggca acgctcgcgg agggcttcga cgtcgcatgc tcgttcgtca
     2041 atgatcacgt cgatcgcgct gcgctcacat tattgagcga tggcggcgtg cggctgatcg
     2101 cgctgcgctc tgccggctac aaccacgtcg atctagaagc tgccaccgag ctcggcttga
     2161 ccgtggtgcg cgtgccggag tactcgccgt acgcggtcgc ggagcatgcg gtcgcgctcg
     2221 tgctcacgct tgatcgcaag atccatcgcg cgtatgcgcg cgtgcgggaa ggcaacttct
     2281 cgctcgacgg gctgctcggc ttcgatctcc acgacaagac cgtcggcatc gttggtaccg
     2341 gacgcatcgg tgcggctgct gcgcgcatct tcgcgggctt tggctgccgc gtactcgcct
     2401 acgatttgcg cccgcagtcc gagctgcctg tcgagtacgt gccgctggac gagctctacg
     2461 cacgctccga catcatctcg cttcacgtcc cgctcacgcc ggcgacacat cacatgatcg
     2521 acgcggccgc gctcgcgcgc atgcagcccg gcatcatgct gatcaacacg agccgtggcg
     2581 cgttgatcga cagccgcgcg ttgatcagcg cactcaagac ctgccacatc ggttctgccg
     2641 gtctcgatgt ctatgaagaa gaggagggcg tgttcttcga agacctgtcg gacaaggtgc
     2701 tgcaggatga tgtactcgcg cgcctgctca cgtttcccaa cgtcgttatc acctcgcacc
     2761 aggggttctt cacccgcgag gccctggcgg cgatcgcgtc gacgacgtta gcgaacgtcg
     2821 acgcgttcga acggggtgag ccgctggtca acgaggttcg agctcagcgc cgttgatcag
     2881 agcgatgcga tccactcgcg gatcgcacgc tcggcctgcg gcatgttcgt gtgcactgcc
     2941 gcgtgctgac gctggaactt gatctcgtga gtctgctcga gctgccgtag caaacgaccg
     3001 acgtctttta gcgccggcaa gataccgagc agcttgtact cgtcacgtcc gtgcacgaat
     3061 gcaatgtcgg tcactcggat ccatgaagag cgcccacgat gcacgaagtg cacgccccat
     3121 tcctcggtgt agatgttgag gtggaagcgc gtcgctcgcg tctcgtcgaa atcggtcggt
     3181 gcgcggtcat tcatcacgtc gagtgcgatc tcgacgtgcc agcgtgttgg cgacgacatg
     3241 ccgcgcgtgc gcagccacgt cgctgctgcg agtgcacctg ccgtcgtggt cgacgcgcca
     3301 ccactactgc ttgctgctac gggcttgcgt tcgacagctc gcctgccgct tgcagctcgc
     3361 tcgcgcgaac tactactcat gagctttcac ctctatatcc cgatcgtagt gcttgcgagc
     3421 ctcgatcgac tcgcagaagc gatccgcgca gcgctgctag gtcagctcaa ctaacccgag
     3481 gtgtggcaga caagcacgat cgggcgcgtg cgcacactgc agtcaacggg gtcgagcccc
     3541 ggcgacacac ctcagcggtg cgcacgcggg ggacgttgat gatcgtaggc agactgtgta
     3601 acagcagatc gagcagtggg ttctcacaag atcctggtcg tcgatgacac gccggcgaat
     3661 ctggtggctg ccgaagcggc cctcgcaccg ctcgagcggc cgatcgtgat ggcgcagtcc
     3721 ggtgaagagg cgctcggcca tctgctcgac caggacttcg cgctcgcatt gctcgacgtg
     3781 cacatgccaa acatcgatgg ctacgagctc gcgcgcatga tccgctcgcg cgagcgcacc
     3841 gagcacatgc cgatcatctt catcacggca cacgcgcacg atgaagcgtc ggtgttgcgc
     3901 gcgtatgacc tcggcgcagt cgatttcctg tttcggccgg tgattccggc agtgcttcgc
     3961 gcgaaggcat cggtgctcgt caccttacaa gagcgcaatg tagagctcgc gaacgaacgt
     4021 gtcgaacgtg atcgcgagca ggccgcacat caagagctcg cgcggctcaa ccgggcgctc
     4081 gccgagtacg atcggcgcaa agacagcttt atcgcgatcc tcgcgcacga gctccgcaat
     4141 ccgctcgcgt cgttgcgtac gtgtgtcgat ctcatccgcg cggctccaac gcaaccgctc
     4201 acgccgcgac agatcacggc gctggaccga caaacgaacg tgctgatgcg gctcgtcgag
     4261 gacttgctcg acgtctcccg catcaaggca gataagatcg agctgcgacc ggagctcgtc
     4321 gacctcgtcg gtctggtcga ggcggcgat
//
LOCUS       DASUFX010000805        16600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1381997, whole genome shotgun sequence.
ACCESSION   DASUFX010000805 DASUFX010000000
VERSION     DASUFX010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16600
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381997"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VFV99_32545"
     CDS             <1..1379
                     /locus_tag="VFV99_32545"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5064166.1"
                     /translation="PASRLYWNELYLDLRKLAPDVELALPPAPPIVADQPIDYRALYR
                     HRRPLIDECVKRLLVNESFATGADAWARDKGAYDYAAFRALGEQQRTGWRQWPARLRD
                     GTAFAGTREAALALGAEAARIDTHVVSQWLMQSQLQQLQGGNVSLYLDLPVGVNCDSY
                     EVWRWRNLFLIDLAAGAPPDALFLGGQNWGLPPLSPIALRRDRYRYFIQCVRHHMKVA
                     GMLRIDHVMGLFRLYCVPAGRSATDGVYLRYHADELLAIATLESTRACCAIAGEDLGT
                     VPEYVRPAMHRHGMFRLHVGQWAFPQHSGEAPTPSPPEAVASLNTHDTATFGGWWRGT
                     DIDDKRDLGLVDEAHERGERHERERQKAALLAFADAKIENDTLTEVERAMVATTTDLA
                     IGGAEIVLVSLDDLVLDPVPHNVPGTIHERPNWQRRVQSWAESLDPERATPAAAAAIA
                     ALVAARPR"
     gene            1472..1636
                     /locus_tag="VFV99_32550"
     CDS             1472..1636
                     /locus_tag="VFV99_32550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064167.1"
                     /translation="MAESESSGSTGIVAIFAILLMIVIAGFIAWRAGVFGGGDSHKSL
                     DINVNTPSSN"
     gene            1800..2726
                     /locus_tag="VFV99_32555"
     CDS             1800..2726
                     /locus_tag="VFV99_32555"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX5064168.1"
                     /translation="MRHLAVFAMFAGCSFSHGVSGGFDDAPNGGSDAPMIDAFVKLDS
                     GVADMSTTDMMTTLDSDGDGVLDAVDNCPTIANTDQRDHDGDLHGDACDHCPHLASTA
                     DPDGDFDGVGDACDPRPSMGGDSRALFEGFYDATSINGWTNNGAGNWSVANGKLTQSS
                     TTKDTQLVIPGNITRAAATVSATVIQLGSGTGFNTPHVSVSSGVGNNQSYWCSVVDEG
                     VNNNKVYASTFYPVNQTNLPNTDWNGTFQAGSVVQETTTLSGSNNICQVVQGGTQAQV
                     SAPIGNAGGQVQLGMRNLSASYDYIFIVSIGN"
     gene            complement(2786..4618)
                     /gene="typA"
                     /locus_tag="VFV99_32560"
     CDS             complement(2786..4618)
                     /gene="typA"
                     /locus_tag="VFV99_32560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5064169.1"
                     /translation="MSSQSIRNVAIIAHVDHGKTTLVDGLLKQAGTFRTGEVVAERAL
                     DSNDLERERGITILSKCTAVTWKGTRINIVDTPGHADFGGEVERVLGMVDCVLLLVDA
                     YEGAMPQTRFVTQKAFEMGLRPIVVVNKIDRPGVDPHDVMDQVFELFVSLGATDEQLD
                     FPVIYASGRQGYAIRELGDTPTDLGPMLDLIVEKVPPAKGDPDAPLVMQVATLDYDDY
                     LGYMAIGRMRAGRCKVGDRVLLAHRNGKKEEFRVQKVLGFQGLKRFELAEAIAGNIVA
                     ITGMEELNVGETITSIAEPTILPLLKVDAPTITMNFRVNDGPFAGKEGKYVTSRNLRE
                     RLEREIKSNVALRVEETEDAGVFKVSGRGELHLSVLIETMRREGYELCVSQPRVILQT
                     DENGRTTEPYEDAVIDLDEAYVGAVVAELNRRLGVMREMRPSAPGRTRLEYRIPARGL
                     IGYRSQFMTDTRGTGVLYTQFAEYGPQGQDIRTRQNGVLISSEEGVTNAYGLFYLQER
                     GQLFVAPGVKNYSGMIVGIHARDNDLVVNPNKVKKLTNIRTTAADEKLVIAPPRQLTL
                     EYALEFINDDELVEVTPATIRLRKAVLDHNVRKRFEKKPAEIDD"
     gene            complement(4618..5175)
                     /locus_tag="VFV99_32565"
     CDS             complement(4618..5175)
                     /locus_tag="VFV99_32565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064170.1"
                     /translation="MLRSLLLLAVLGCQGKKAAPPPDPVGIQVRDASGVLVAELKPIR
                     PCRGSVGPVDLIVGGPPLVATYGGTQWAGSAAENGTVLTRDGEAVARIFPVGDPKSAG
                     VLDMHGVALARITVTGQTASVINAATVPVRKLSLAGTEIKTDEPAFTITGTQDLVLAG
                     LLSAPELSPEVRILAACERVLAKDK"
     gene            5262..6716
                     /locus_tag="VFV99_32570"
     CDS             5262..6716
                     /locus_tag="VFV99_32570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064171.1"
                     /translation="MKRQLPLLVFLLACGRSAGTSENAGPRREDGGVARAEAPAALAP
                     LALGMPDLADFAYRARTGQDAFKLARASEAKGDWTQVAARCREALTADPNHLDASYLY
                     AVALAKTGGGPEQILTPLTKAVAADYAKWGQASLDQPALQPFLASTIGQAWRQRVEAD
                     RAPFLAALARSLIVTSQNDLFAYDREGKRWYRLTRTGGAVVATLHVHSQKKLAYVTRE
                     KVKDGAKQRTKVGIGVVDLATGKTRRSIEVPHEVPASATLRIGYAERPTPGFIVRAGK
                     VSWRLVENGKLDLVKLPANAKADAPALLADMVRMDVTGRTARIDRTAVANVAADWDDN
                     SLASAIKIGRSKKVVTVPSPGLIDGDTATWNTDGTELAFVAQLSDTCAPNVATAAAFV
                     ADGATGSVHELERALGGIAVEWYGERTLAIAGDHGVSIVALDGSAPLVLTGADGLVTP
                     RRKPKCSPEPIVEEPIAEDEESTTEPSDAGVDAK"
     gene            6895..7305
                     /locus_tag="VFV99_32575"
     CDS             6895..7305
                     /locus_tag="VFV99_32575"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5064172.1"
                     /translation="MQLRQMATAGLGGIAATVVDMSSLLLMVKHGAPVALAAFIAAAC
                     GAAVGFTMNKYIAFRDRSPINVKQLGRFAGVAVATALLMAAAMQVFAVHFGISVVVAK
                     LICSALVFIAWTYPAQRRLVFRPRSQISPWMSLS"
     gene            7315..8076
                     /locus_tag="VFV99_32580"
     CDS             7315..8076
                     /locus_tag="VFV99_32580"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX5064173.1"
                     /translation="MTALLPFVDLSIVVPAYNEELRLPPTLAKLKAFLDTQPLRYEIV
                     VVDDGSKDNTIGAVNAAMATIPNLRLVEQKPNRGKGAAVRRGMLAARGQIRVMCDADG
                     SMPPEELPKLLAPIVACTAEIAIGSRYVEGAKTDKKQPFYRVLWSRLCNRVIQKSLVP
                     GVRDTQCGFKAFTAEAARDLFRYGRIDGWAFDLEILALARRRGFSIAEVGVEWMDDGR
                     SRVNPLKDMWKVIKEALTIRRNLRRGVYESLPAAL"
     gene            8105..8456
                     /gene="rnpB"
                     /locus_tag="VFV99_32585"
     ncRNA           8105..8456
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFV99_32585"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8610..9920
                     /locus_tag="VFV99_32590"
     CDS             8610..9920
                     /locus_tag="VFV99_32590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064174.1"
                     /translation="MHELVNALWRALHELGSDASTNEIEQWGFSIHAALSAPGREFHN
                     HDHVIGLAKEGDPLEVIAALYHDAVYIQVDHGPPRSMRAEMDRVLMHADGGWRILSAA
                     ADSEVSADVLAVFGRRVGDVVTSTAGLNEFASALVCSIQLQKALDRHQRIAVAACIEQ
                     TIPFRSNPVPDLRDRVASLGVTGDELEEMIKRAVRVGNRDVANFSDNDPASFLDNTWK
                     LLPESNPALHSPMVYTVRDYRGALQKMESFLAWLPADRVFHTWADEPKPEVHARRVAR
                     TTGNLELAVRYLRAKLYSIALVEALSELSGGDVPVDYFMGGVKDRERPDMKRIENYLP
                     RLSAAKDLDPPLQRLLAEGRASESSFDTKPSPVGAFLMETVGEAAVMKGVEMAKRWWA
                     GAGDAQSFISAQPPKQVAAIARAAANIIDTRRDRLFALAERLES"
     gene            9917..10426
                     /locus_tag="VFV99_32595"
     CDS             9917..10426
                     /locus_tag="VFV99_32595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064175.1"
                     /translation="MMRSAHRVLYFTLAFAVGCGSDDVDLTGVYRVDADVASMPCGAD
                     QPVMTPPPYLKFEQNEIFGSKYYSMLECSDEAATNCSGGGLFGDSFAEPIDGGWRGVI
                     SSASGTTSCYYSFLEQTAKVDGMKLVVDSNEYSEMTADATNCTAEEAEKRGKTMPCTT
                     HERIDATKL"
     gene            10410..10901
                     /locus_tag="VFV99_32600"
     CDS             10410..10901
                     /locus_tag="VFV99_32600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064176.1"
                     /translation="MRRSCELVVALWCAGCAQSDYVDLTGVYRVDAELSSEPCGIDQP
                     VAQPWPYLKFQESTTLSGFWLLRCSDRATMNCGGDMFLDAFPQPTDAGWIGELSDASN
                     TTPCVLLYSVRSARLDGDHLTVGIEDHSEEVPDADCSPREAELRSTLMPCTKHERFGA
                     TRL"
     gene            complement(10902..11654)
                     /locus_tag="VFV99_32605"
     CDS             complement(10902..11654)
                     /locus_tag="VFV99_32605"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX5064177.1"
                     /translation="MRDQLKNLLTLQTCDIKVRELDTAAKQLPAKLDPLRRDLAKLQG
                     MLDGERAKLAETEAWRKSQQELIDREREALKSAQTKLQASKNSKEFNAASREVENKRK
                     SIQDRESELKKVAEASNASNQQLSARDADVQKLRDELASSEAAMADQVKSLEEQLGEA
                     RTARDAARAAVEKNWVKTYDTLVGKRGYAVAPVIKGVCQGCHMALPPQLNNILARMES
                     IETCPRCGRLVYRKELLEPPPSADAPKPDGQS"
     gene            complement(11705..11778)
                     /locus_tag="VFV99_32610"
     tRNA            complement(11705..11778)
                     /locus_tag="VFV99_32610"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11742..11744),aa:Ile,seq:cat)
     gene            11914..12660
                     /locus_tag="VFV99_32615"
     CDS             11914..12660
                     /locus_tag="VFV99_32615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064178.1"
                     /translation="MNLGNIARVVAVAAACLAAVGSATSNNNSPAVQAQNANMSPPPP
                     QQMEPSRALGLWRSTFGAVKIEADNSKGGISSGAVQGIWVYQRRGQEVIGYFSGSLRG
                     NVLDFRWQEPGSADAPPLVGAGYLVFEQTGRQYSGRWWSDKRDRVGDWNGWRQANAAA
                     EQPQYGTYGGNVYGGQTYGQTPYGPQQPATYPQQPQQPQYPQQPQQQPQYPQQPQYPQ
                     QPQQQPQQPPPSPQQPYYPQQPQPYYPPPR"
     gene            12759..13643
                     /gene="dapF"
                     /locus_tag="VFV99_32620"
     CDS             12759..13643
                     /gene="dapF"
                     /locus_tag="VFV99_32620"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5064179.1"
                     /translation="MRFAKYHGLGNDFLVVDLRNAQGDEAKAVQEPAVVKALCDRQFG
                     VGGDGVLAVLPGDPGADARMRVLNSDGSEAEMCGNGIRCVAKELFDRGGVRKDRIAID
                     TGAGRLVCAIEKRGDVAQTVTVEMGRPRLTRGEIPMTGPASERCIEQPIEVRGQPTRN
                     VTCVSMGNPHAVAFVDGNLRALAESAGPLVENHAWFPNRTNAEFARVISPREIELVVW
                     ERGCGITLACGTGACATVVAAVITGRAEEGTEVLVHLPGGDLVITVEKGLSNVLMRGP
                     ALHVFDGELTPRQVAPRP"
     gene            complement(13687..13854)
                     /locus_tag="VFV99_32625"
     CDS             complement(13687..13854)
                     /locus_tag="VFV99_32625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064180.1"
                     /translation="MQWAGRYRDATAIVDAWSNASSTRAPWIHVQGTTKPRTRPSKQR
                     QRADAAKPEGK"
     gene            complement(13926..14747)
                     /gene="secG"
                     /locus_tag="VFV99_32630"
     CDS             complement(13926..14747)
                     /gene="secG"
                     /locus_tag="VFV99_32630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5064181.1"
                     /translation="MSTLITILHVIVCLFLMLTVLLQSGKGGGMGGAFGGSSTGTVFG
                     GSGASSFLQKLTAGAAVVFMATSMVLAFIASHDSSDALEKFGSEQAKLATQKEEAKEK
                     ALQQGSGSAGSAGSASMMLEPTTPTPPTGSAAPTPPTGSATAPAGSAAPAAGSATVPA
                     GSAAPAAGSATATPPAAKPQDATKPATAPTTKPQDAAKPAAPATKPADATKAGDATKP
                     STPATKPADATKPADATKPADATKPATPATKPADTTKPTEAPKPADSSPNAPKPN"
     gene            complement(14792..15556)
                     /gene="tpiA"
                     /locus_tag="VFV99_32635"
     CDS             complement(14792..15556)
                     /gene="tpiA"
                     /locus_tag="VFV99_32635"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5064182.1"
                     /translation="MRIPFVVGNWKLNKTINEALALVTELKNQLGAVKGVAVGVAPTF
                     LAINAVAKRLEGSAIVTCGQDCFWENSGAFTGEVSAPLLSDAGATWSIVGHSERRQFF
                     GDTNESVGKKARAVLAGGLGVIVCVGEMLAERDGGRTLDVVDAQLAGGLEGIDAAVAA
                     QRLVIAYEPVWAIGTGRTATPAQAQEVHAHIRKRLGERFGAQTAAAIRIQYGGSVKPS
                     NAEALMAEADIDGALVGGASLEAADFVAIVKASRPV"
     gene            complement(15743..>16600)
                     /locus_tag="VFV99_32640"
     CDS             complement(15743..>16600)
                     /locus_tag="VFV99_32640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064183.1"
                     /translation="APQVVQRQLNDPMMPPSQMRAGLSEAVDVVFRKALQPNPKKRWA
                     SASTFAVALGRALERMSGEMRVPPTEQDVAALQAESVLGPTKGIDEASGVVPMASIRP
                     EVSGRVRAAHLRVLSKILQHHIGESGIAKMKNERPDLANVLASTLAPLAWVELADLVA
                     ALDLTKRTLPNALVARKVGRGTMSATFARLFGADPSSLSPETVMAALPTFWDRYHEWG
                     GVVVGVKPNHVDVTLAGFAGSTEVCLLVSAELERIIELTGAAGVTSTHPRCRCTGNEQ
                     CEFTLSWST"
BASE COUNT         2727 a         5498 c         5512 g         2863 t
ORIGIN      
        1 tgccggcgag tcgcctgtac tggaacgagc tgtatctcga tctgcgcaag ctcgcgcccg
       61 acgtcgagct cgcgctgccg ccggcgccac cgatcgtcgc tgaccagccg atcgactatc
      121 gcgcgctgta tcgccatcgc cgcccgctca tcgacgagtg cgtgaagcgc ttgctcgtga
      181 acgagtcgtt cgcgaccggc gccgacgctt gggcgcgtga caagggcgca tatgactacg
      241 ccgcgtttcg cgcgctcggc gagcagcaac gcactggctg gcgccagtgg ccggcgaggc
      301 tgcgtgatgg caccgcgttc gcgggcacgc gtgaggccgc actcgcgctc ggagccgaag
      361 ccgcgcgcat cgacacccac gtcgtctcgc aatggctcat gcaatcgcaa ttgcagcagc
      421 tgcaaggtgg caacgtcagc ctctatctcg atctgcccgt cggcgtgaac tgcgattcgt
      481 acgaggtgtg gcgctggcgc aatctgttcc tgatcgacct cgcggccggc gcgccgccgg
      541 atgcgctgtt ccttggcggc cagaattggg gcctgccacc gctgtcaccg atcgcgttgc
      601 gccgcgatcg ctatcgctac ttcatccagt gcgtgcggca tcacatgaag gtcgccggga
      661 tgctccgcat cgatcacgtg atgggcctgt tccggctcta ctgcgtgccc gcggggcgct
      721 cggccaccga cggcgtgtat ctgcgctatc acgccgacga gctgctcgcg attgccacgc
      781 tcgaatcgac acgcgcctgc tgcgcgatcg ccggcgagga tctcggaacg gtgcccgagt
      841 acgtgcgccc ggcgatgcat cgccacggca tgttccgcct ccacgtcggt caatgggcgt
      901 tcccacagca ttccggcgag gcaccgacgc cgtcgccgcc ggaagccgtc gcgtcgctca
      961 acacgcacga cacggcgacg ttcggcggct ggtggcgcgg cactgacatc gacgacaaac
     1021 gcgacctcgg cctcgtcgat gaagcgcacg agcgcggcga gcgtcacgag cgcgaacgcc
     1081 agaaggcggc gctgctcgcg ttcgccgacg cgaagatcga gaacgacaca ctcaccgagg
     1141 tcgagcgcgc gatggtcgcg accacgaccg atctcgcgat cggcggcgcc gagatcgtgc
     1201 tggtctcgct cgacgatctg gtgctcgatc cggtgccgca caacgtgccg ggcacgattc
     1261 acgagcggcc gaactggcag cgccgcgtgc agagctgggc cgagtctctc gatcccgagc
     1321 gcgccacacc tgccgcggcc gccgcgattg ccgcgctggt cgccgcgcgc ccccgctaac
     1381 gccgtcgcgg aacgcacgaa aaatctcctg cggcgatctc gcaaggcgct ggcacgtgga
     1441 ctgctccttt ctactgacag gaggaactcc catggccgaa tcagaaagca gcggcagcac
     1501 cggcatcgtc gcgatcttcg cgatccttct catgatcgtg atcgccggct tcatcgcgtg
     1561 gcgagcgggc gtctttggcg gcggcgactc gcacaagtcg ctcgacatca acgtgaacac
     1621 acccagctcc aactaacggg cgggtctgtc acggccgggc ggtccatcgc agtcgcgcct
     1681 tgttacatcg ccgggaagct tcgattcgct gtcggttagc gatgttgcgc gcgtgggcgg
     1741 gctcacggac ggtgaacggg actccaaatt ccggtgaaag aacctgtacg atacccccca
     1801 tgcgccacct cgcagtgttc gcaatgtttg cgggctgcag cttttcgcat ggcgtgagcg
     1861 gtgggttcga cgatgcccct aacgggggga gcgacgcgcc gatgatcgac gcgttcgtga
     1921 agctggactc gggcgtcgcc gatatgtcga cgaccgacat gatgacgact ctcgacagcg
     1981 acggcgacgg tgttctcgac gccgtagata actgtccgac gatcgcgaac accgaccagc
     2041 gtgatcacga cggcgacttg cacggcgacg cctgcgatca ttgcccgcat ctcgcgagca
     2101 cggcggatcc ggacggcgat ttcgacggcg tcggcgacgc ctgcgatccg cggccgagca
     2161 tgggcggcga ttcgcgcgcg ctgttcgaag ggttctacga cgccacttcg atcaacggct
     2221 ggaccaacaa cggcgccggt aactggtcgg tcgcgaatgg caagctcacc caatcgtcga
     2281 cgaccaaaga cacccagctc gtcatcccgg ggaacatcac gcgcgctgcg gcgaccgtga
     2341 gcgcgacggt gatccagctc ggctcgggga ccggcttcaa cacgccgcac gtgtctgtga
     2401 gctcgggcgt cggcaacaac cagtcgtact ggtgctcggt tgtcgacgag ggcgtcaaca
     2461 acaacaaggt ctacgcgagc acgttctatc cggtcaacca gacaaacctt ccaaacaccg
     2521 actggaacgg cacgttccag gctggcagcg tggtgcagga gacgacgacg ctgagcggct
     2581 cgaacaatat ttgtcaggtc gtgcagggcg gcacgcaggc gcaagtctcc gccccgatcg
     2641 gcaacgcggg aggtcaggtg cagctcggga tgcgcaacct gtccgcgagc tacgactaca
     2701 tcttcatcgt ctcgatcggc aattagagct gagcgccgac cccggccgac ccaccgacgt
     2761 gccctcgtgc ggcacgacgt ggcaatcagt cgtcgatctc ggcgggcttc ttctcgaacc
     2821 gcttgcgcac gttgtggtcg aggacggctt tgcgcaggcg aatcgttgcc ggcgtcacct
     2881 cgacgagctc gtcgtcattg atgaactcga gcgcgtactc gagcgtgagc tgacgcggtg
     2941 gcgcgatgac gagcttctcg tcggccgccg tcgtgcggat gttcgtcagc ttcttgacct
     3001 tgttcgggtt gacgacgagg tcgttgtcgc gcgcgtggat gccgacgatc atgccgctgt
     3061 agttcttcac gccgggagcg acgaacaact ggccgcgctc ctggaggtag aacaagccgt
     3121 aggcgttcgt cacgccctct tcgctggaga tcaggacgcc gttctgacgg gtgcggatgt
     3181 cttggccctg ggggccgtac tcggcgaact gcgtgtagag cacgccggta ccgcgcgtgt
     3241 cggtcatgaa ctgcgagcgg tagccaatga ggccacgcgc cggaatccgg tactcgagtc
     3301 gcgtgcgacc cggtgccgac ggccgcatct cgcgcatcac gccgagtcgg cggtttagct
     3361 cggcgacaac tgcgccgacg taagcctcgt cgagatcgat gacggcgtcc tcgtagggct
     3421 cggtcgtgcg accgttctcg tcggtctgca ggatcacgcg cggctggctg acgcacagct
     3481 cgtagccctc gcggcgcatc gtctcgatca gcaccgacag gtggagctcg ccgcgccccg
     3541 agaccttgaa tacgcccgca tcctcggtct cttcgacgcg gagtgcgacg ttgctcttga
     3601 tctcgcgctc gagccgctcg cgcaggttgc ggctcgtgac gtacttgcct tccttgcccg
     3661 caaacggacc gtcgttgacg cggaagttca tcgtgatcgt cggcgcgtcg accttcagca
     3721 gcggcaagat ggtcggttct gcgatcgagg tgatggtctc gccaacgttg agctcttcca
     3781 tgccggtgat cgcgacgatg ttgccggcga tcgcctcggc gagctcgaac cgcttgaggc
     3841 cctgaaagcc gagtaccttc tggacgcgga actcttcctt cttgccgttg cggtgcgcga
     3901 gcagcacgcg atcgccgacc ttgcagcggc cggcgcgcat gcggccgatc gccatgtagc
     3961 cgaggtagtc gtcgtaatcg agcgtcgcga cctgcatgac gagcggcgcg tccggatccc
     4021 ccttcgccgg cggcaccttc tcgacgatca ggtcgagcat cgggccgagg tcggtcggtg
     4081 tgtcgccgag ctcgcggatc gcgtagccct gacggcccga cgcgtagatc accgggaagt
     4141 cgagctgctc gtcggtcgcg ccgagggaca cgaacagctc gaacacctga tccatgacgt
     4201 cgtgcggatc gacaccgggg cgatcgatct tgttgacgac gacgatcggg cgcaggccca
     4261 tctcgaacgc cttctgcgtc acgaaccggg tctgcggcat cgcgccctcg taggcatcga
     4321 cgagcagcag cacgcaatcg accatgccga gcacgcgctc gacctcgccg ccgaagtcgg
     4381 cgtgacccgg cgtatcgacg atgttgatgc gcgtgccttt ccaggtcacc gcggtgcact
     4441 tgctgaggat cgtgatgccg cgctcgcgct cgagatcgtt cgaatcgagc gcgcgttcgg
     4501 cgaccacctc accggtgcgg aatgtgcccg cttgcttgag caagccatcg acgagcgtgg
     4561 tcttgccgtg gtcgacgtgg gcgatgatgg cgacgttacg gatgctctgc gaagacatca
     4621 cttatccttc gcgagcacgc gctcgcacgc cgccaggatg cggacttcag gcgacaactc
     4681 tggtgccgac aacaggccgg cgagcacgag atcctgcgtg cccgtgatcg tgaatgcggg
     4741 ttcgtctgtc ttgatctcgg tgccggcaag gctcagcttg cggaccggca cggtggccgc
     4801 gttgatcacc gacgcggtct gcccggtcac cgtgatgcgc gcgagcgcga cgccatgcat
     4861 gtcgagcacc ccagcgctct tcggatcgcc gaccgggaag atgcgcgcga cggcctcacc
     4921 atcgcgcgtg agcacggtgc cgttctccgc ggccgagccc gcccactgcg tgccgccgta
     4981 ggtcgcgacc agtggtgggc cgccgacgat cagatcgacc gggccgaccg aaccgcggca
     5041 cggccggatt ggcttcagct cggcgaccag cacgcctgac gcatcgcgca cctggatacc
     5101 caccgggtcg ggtggtgggg ccgccttctt gccttggcaa ccaaggacgg ccagtaacag
     5161 caaactacgc agcatcgggg gcggcggttt gtatcaggcg gacgggagta gagcaatgct
     5221 aagtcttcgc gatccctgcg agtccgtgat aaggcgtcgg aatgaagcgg cagctgccgc
     5281 tgctcgtgtt tctcctcgct tgcggccgca gcgccggcac ctcggaaaac gccggaccac
     5341 ggcgcgagga tggcggggtt gcgcgcgccg aggccccagc cgcgttggcg ccgctcgcgc
     5401 tcggcatgcc ggacctcgcg gatttcgcgt accgcgcgcg caccggccag gacgcgttca
     5461 agctcgcgcg cgccagcgaa gcgaaaggcg actggacgca agtcgctgcg cggtgccgcg
     5521 aagcactcac cgcggatccg aaccatctcg atgcttcgta cctctacgcg gtcgcgctcg
     5581 cgaagaccgg cggcggtccc gagcaaattc tcacgccgct cacgaaggcg gtcgccgcgg
     5641 actacgcgaa gtggggacag gcatcgctcg atcagcctgc gctgcagccg tttctcgcgt
     5701 cgactatcgg ccaggcgtgg cgacagcgcg tggaggccga tcgtgcgccg ttcttggcgg
     5761 cgctcgcgcg ctcgctgatc gtcacgtcgc aaaacgatct gtttgcgtac gaccgcgaag
     5821 gtaagcgctg gtatcggctg acgcgcaccg gtggcgcagt tgtcgcgacg ctccacgtgc
     5881 actcgcagaa gaagctcgcg tacgtgacgc gcgagaaggt caaagacggc gcgaagcagc
     5941 gcacgaaggt cggcatcggt gtcgtcgatc tcgcgacggg caagacgcgc cgctcgatcg
     6001 aagtgccgca cgaagtgccc gcgagcgcga cgctgcgcat cggctacgca gagaggccaa
     6061 cgcctggctt catcgtgcgg gcgggcaagg tgagctggcg cttggtcgag aacggcaagc
     6121 tcgatctcgt gaagctgccg gcaaacgcga aggccgacgc gccggcgctc ctcgccgaca
     6181 tggtgcgcat ggacgtcacc ggccgcacgg cgcgcatcga tcgcaccgca gtcgcgaacg
     6241 tcgcggccga ttgggatgac aactcgctcg cgagcgcgat caagatcggc cgctcgaaga
     6301 aggtcgtcac cgtgccgagc ccgggcctca tcgatggcga caccgcgacc tggaacacag
     6361 atggcaccga gctggcgttc gtcgcgcagc tctccgatac gtgcgcaccg aacgtcgcca
     6421 ccgcagccgc gttcgttgcc gatggcgcga ccggcagcgt gcacgagctc gagcgcgcgc
     6481 tcggtgggat cgcggtcgag tggtatggcg aacgcacgct cgcgatcgcg ggtgatcacg
     6541 gcgtatcgat cgtggcgctc gatggcagcg cgccgctcgt gctgaccggc gccgatggcc
     6601 tcgtcacgcc gcgccgtaaa ccgaagtgct cgccggagcc gatcgtcgaa gagccgatcg
     6661 cggaagacga agagagcacg accgagccga gcgacgccgg cgtcgacgcg aagtaggcga
     6721 cgtaggcgac gtaggcgtgt gcgacgagcg ggggttattc acggatcacg cgcagttcac
     6781 gaaccggtag ctaccaccgg tttcagatag cttttcacca ccttccgaca cgcctcggcc
     6841 agtggtttcg cgcgtttgcc acgtggcaca tccgctgctt tttgtctcgc catcatgcaa
     6901 ctgcgtcaga tggcaacggc cggtttgggt ggcatcgccg ccaccgtcgt cgacatgtcg
     6961 tcgcttctcc tgatggtgaa gcacggcgcg ccggtggcac tcgccgcgtt catcgccgca
     7021 gcgtgtggtg ccgcggtggg cttcacgatg aacaagtaca tcgcgtttcg tgatcgctcg
     7081 ccgatcaacg tcaagcagct cggtcgcttc gccggtgtcg ccgtggcgac cgcgctcttg
     7141 atggcagctg cgatgcaggt gttcgccgtg cacttcggca taagcgtcgt cgtcgccaag
     7201 ctcatctgtt cggcgctcgt gttcattgcg tggacgtatc ccgcacagcg ccgcctcgtg
     7261 ttccgtccgc gttcgcaaat ctcgccgtgg atgtctcttt cgtaggagct cgccatgact
     7321 gcactacttc cattcgtcga cctgtcgatc gttgttcctg cctataacga agagttgcgc
     7381 ctgccgccga cgctcgccaa gttgaaggcg ttcctcgaca cgcagccgct tcgctacgag
     7441 atcgtcgttg tcgacgacgg ctcgaaggac aacacgatcg gtgccgtcaa tgcagcgatg
     7501 gcgacgattc cgaaccttcg cctcgtcgag cagaagccga accgtggcaa gggtgctgcg
     7561 gtccgacgcg gcatgctcgc ggcgcgcggt cagatccgcg tgatgtgcga tgcggacggc
     7621 tcgatgccgc cagaagagct gccgaagctg ctcgcgccga tcgtcgcgtg cacggcagag
     7681 atcgcgatcg gttcgcgcta cgtcgaaggt gcgaagacgg acaagaagca gccgttctat
     7741 cgcgtgctgt ggagccgcct ctgcaaccgc gtgatccaga agtcgctcgt ccccggcgtg
     7801 cgagacacgc agtgcggctt caaggcgttc accgccgaag cggcgcgtga cctgttccgc
     7861 tacggccgca tcgacggctg ggcgttcgat ctcgagatcc tcgcgctcgc gcgccgccgc
     7921 ggcttctcga tcgccgaagt tggcgtcgag tggatggacg atggtcgctc gcgcgtcaac
     7981 ccgctcaaag acatgtggaa agtgatcaaa gaagcgctga cgatccgccg caacctccgc
     8041 cgcggcgtct acgagtcctt gcctgccgcc ctctaggtga gtagggttac gccgctcact
     8101 cgtcggagag ggagaggtga ccgccggcaa cggcgcggca acgcgcccga gcgggaggaa
     8161 agtccgagct tcgtagggca gggtgctggt taacgaccag tcgaggtgac tcgcaggaaa
     8221 gtgcaacaga aagcaaaccg cctgtcgcaa ggcaggtaag ggtgaaacgg tgcggtaaga
     8281 gcgcaccgcc tcgttggtga caacgagggc aaggcaaacc ccacccgaag caagaccaga
     8341 caggggtcgt gtggcccgca cgatgactcc gggttggtcg ctcgagtccg ccggcaacga
     8401 cggacctaga agaatggtcg ccagatacag aactcggctt atcgccctct ccgacgcgtg
     8461 agccttacct cgaacgcaac cactcgcgac aacgaaggtt gtggtccgtc cgctgcaact
     8521 actcgcggca ccggatcacc cgaccgctca cccacgcgca catcgccgcg tgataccgtg
     8581 gttctcgggc tatggatcag cggtcgccgg tgcatgagct ggtaaacgcg ttgtggcgtg
     8641 ccctccacga gctgggctcc gacgcgtcga ccaacgaaat cgagcagtgg ggcttctcga
     8701 tccacgccgc gttgtcggcg ccgggccgcg aattccacaa ccacgaccac gtgatcggtc
     8761 tcgccaagga aggcgatccg ctcgaggtca tcgccgcgct ctaccacgat gccgtgtaca
     8821 tccaggtcga tcacggcccg ccgcgctcga tgcgcgccga gatggatcgc gtgctgatgc
     8881 acgccgacgg tggctggcgc atcctctcgg ccgcagccga cagcgaggtc agtgccgacg
     8941 tgctcgcggt gttcggtcgc cgcgtcggtg atgtcgtcac gtcgacggcg ggcctcaacg
     9001 agttcgcgtc cgcgctcgtc tgttcgatcc agctgcaaaa ggcgctcgat cgccaccagc
     9061 gcatcgccgt cgccgcgtgc atcgagcaga cgattccgtt ccgctcgaat ccagtgcccg
     9121 atctgcgcga tcgcgtcgct tccctcggcg ttaccggcga cgagctcgag gagatgatca
     9181 agcgcgccgt gcgcgtcggt aatcgcgacg tcgcaaactt ctccgacaac gatccggcga
     9241 gcttcctcga caacacctgg aagctcctgc ccgagagcaa tccggcgctg cactcgccga
     9301 tggtctacac ggtgcgcgat taccgcggcg cactgcagaa gatggagagc ttcctcgcgt
     9361 ggctccccgc cgaccgcgtg ttccacacgt gggccgacga gccgaagccc gaggttcacg
     9421 cgcgtcgcgt cgcgcgcacg accggcaacc tcgagctggc cgtgcggtac ctgcgcgcga
     9481 agctgtattc gatcgcgctc gtcgaggcgc tgtccgagct gtccggtggc gacgtcccag
     9541 tcgattactt catgggcggc gtcaaggatc gcgagcgccc ggacatgaag cgcatcgaga
     9601 actatctgcc tcgcctgagc gccgcgaagg atctcgatcc gccgctccag cggctgctcg
     9661 ccgaaggccg cgcctcggag tcgagcttcg acaccaagcc gtcgccggtc ggcgcattcc
     9721 tcatggagac cgtcggtgaa gcggcggtga tgaagggcgt cgagatggcg aagcgctggt
     9781 gggcgggcgc cggcgacgcg caatcgttca tctcggcgca gccaccgaaa caggtcgcag
     9841 cgatcgcacg cgccgcagcg aacatcatcg atacccgtcg cgatcgcctg ttcgcactcg
     9901 cggagcggct cgaatcgtga tgcgcagcgc gcaccgcgtg ctgtacttca cactcgcgtt
     9961 cgcggttggc tgcggctccg acgatgtcga tctgaccggc gtctatcgcg tcgatgccga
    10021 cgtcgcctcg atgccatgcg gcgccgatca gccggtgatg acaccgccgc cttatctcaa
    10081 gttcgaacag aacgagatct tcggctcgaa gtactactcg atgctcgagt gctccgacga
    10141 agccgcgacg aactgctcgg gcggcggtct gttcggcgat tcgttcgccg agccgatcga
    10201 tggcggctgg cgcggcgtga tctcgtcggc gagcggcacg acctcctgct actactcgtt
    10261 cctcgagcag accgcgaagg tcgatggcat gaagctcgtc gtcgattcga acgagtacag
    10321 cgagatgacc gctgacgcga cgaactgcac tgccgaggaa gccgagaagc gcggcaagac
    10381 catgccgtgc accacgcacg agcggatcga tgcgacgaag ctgtgagctc gtcgtcgcgc
    10441 tgtggtgtgc cggttgcgcg cagagtgact acgtcgatct gacaggcgtg tatcgcgtgg
    10501 atgccgagct ctcgtcggaa ccgtgcggga tcgatcagcc ggtcgcgcaa ccatggccgt
    10561 atctaaaatt ccaagagagc accacgctga gcggattctg gctcctgcgc tgttccgatc
    10621 gcgcgacgat gaactgtggc ggcgacatgt ttctcgatgc gtttccgcag cccaccgacg
    10681 ccggctggat cggcgagctc tctgacgcga gcaacaccac accgtgcgta ttgctctaca
    10741 gcgtgcggtc ggcacgactc gacggcgacc atctcactgt cggcatcgag gaccacagcg
    10801 aagaagtgcc ggatgctgac tgctcgccgc gcgaggccga gctacgcagc acgctgatgc
    10861 cgtgcacgaa gcacgaacgg ttcggcgcga cgcgtctgtg actagctctg accgtcgggc
    10921 ttcggcgcgt cggccgacgg aggcggctcg agcaactctt tgcgatagac gaggcggcca
    10981 cagcgcgggc aggtctcgat cgattccatg cgcgcgagga tgttgttgag ctgcggcggc
    11041 agcgccatgt ggcagccctg gcacacgcct ttgatcactg gcgcgaccgc gtagccacgc
    11101 ttgccgacca gcgtgtcgta cgtcttcacc cagttctttt cgaccgcggc acgagcagca
    11161 tcacgcgccg tgcgcgcttc gccgagctgc tcttcgagtg acttgacctg atccgccatc
    11221 gcggcctcgc tcgacgcgag ctcgtcgcgg agcttctgca cgtcggcgtc gcgcgcgctg
    11281 agctgctggt tcgacgcgtt gctcgcctcg gcgaccttct tgagctcgct ttcgcgatcc
    11341 tggatcgact tgcgcttgtt ctcgacctcg cggctcgcgg cgttgaactc tttgctgttc
    11401 ttcgacgcct gcagcttggt ctgcgcgctc ttgagtgcct cgcgctcgcg gtcgatcagc
    11461 tcttgctgag acttccgcca ggcttcggtc tcggcgagct tcgcacgctc gccgtcgagc
    11521 atgccctgga gcttcgcgag gtcgcggcgg agtgggtcga gcttcgcagg tagttgcttc
    11581 gctgctgtgt cgagctcgcg aaccttgatg tcacacgtct gcagggtgag taggttcttc
    11641 aactgatcgc gcacggacag cctttcgctt tgcatctact tctgggcagc tggttttcag
    11701 gtcgtgggcc cacctgggct cgaaccaggg acccaccggt tatgagccgg ccgctctaac
    11761 cgactgagct atgggcccgc gtgtacgtgg atgccactct atgtggaaga ccgcacgtaa
    11821 cgcgagggga cactatcagg ttgccagaaa acgacaacct cccgcgtcac aacagacgct
    11881 gcgtcatctc ggcgcggcgc tatactcgtc gcgatgaacc tcggcaatat cgcacgggtg
    11941 gtagctgtcg cagcggcgtg cctagcggcc gtcgggtcgg cgacttcgaa caacaacagc
    12001 ccggcggtgc aggcgcagaa cgcgaacatg agtccgcctc cgccgcagca gatggaaccg
    12061 tcgcgtgcgc tcggcctgtg gcggtcgacg ttcggcgccg tgaaaatcga ggcggacaat
    12121 agcaagggcg ggatctcgtc gggcgccgtg cagggcatct gggtctacca gcgccgcggc
    12181 caagaagtga tcggttactt ctcgggcagc ctgcgcggca acgtgctcga tttccgctgg
    12241 caagagccgg gcagcgcaga cgcgccgccg cttgtcggtg cgggatactt ggtgttcgaa
    12301 caaacgggtc gccagtactc gggacgctgg tggagcgata agcgcgatcg cgtcggcgac
    12361 tggaacggct ggcgccaggc gaacgcagcg gccgagcagc cgcagtacgg cacatacggc
    12421 ggcaacgtct acggtggaca gacgtacggg caaaccccgt acggcccgca gcaaccggcg
    12481 acgtatccgc agcagccgca gcagccgcaa tatccgcaac agccgcagca gcagccgcag
    12541 tatccgcaac agccgcagta cccgcagcag ccacagcagc agccgcaaca gccgccgccg
    12601 tcgccgcagc agccgtacta cccgcagcag ccgcagccgt actatccgcc gccgcgctga
    12661 gcattatttc cgccaacaga tcagccgact gctcgtagtt tggtgcagat cgcgaggacg
    12721 cttggcgacc gcgatttctt cgcggtaggg tcccgcatat gcggttcgcc aagtaccacg
    12781 gtctcggcaa tgactttctc gtcgtggatc tgcgcaacgc gcagggcgac gaagcgaaag
    12841 ccgtgcaaga gcccgcggtc gtcaaggcgc tgtgcgatcg gcagttcggc gtcggcggtg
    12901 atggcgtgct cgcggtactg cccggcgatc cgggagcaga tgcgcggatg cgcgtactga
    12961 actccgacgg gagcgaagcc gagatgtgtg gcaacggcat tcgctgcgtt gccaaggagc
    13021 tgttcgatcg cggcggcgtg aggaaagatc gcatcgcgat cgataccgga gcagggcgcc
    13081 tcgtatgcgc gatcgagaag cgcggtgatg tggcgcagac cgtgacggtc gagatgggcc
    13141 gtccgcgtct tacgcgcggg gaaattccga tgaccggccc cgcgagcgaa cgctgcatcg
    13201 agcagccgat cgaagtgcgt ggtcagccga cgcgcaacgt cacgtgtgtg tcgatgggca
    13261 atccgcacgc ggtcgcgttc gtcgacggca acctgcgcgc gcttgccgag agcgcgggtc
    13321 ccctcgtcga gaatcacgca tggtttccaa accgcacgaa cgccgagttc gcgcgcgtga
    13381 tcagcccgcg cgagatcgag ctcgtcgtgt gggagcgcgg ctgcggcatc acgctcgcgt
    13441 gcggcaccgg cgcgtgcgcg accgtcgtcg ccgcggtgat cacgggccgc gccgaagagg
    13501 gcaccgaggt gctcgtgcac ttgccgggcg gcgatctcgt catcaccgtc gagaagggcc
    13561 tctcgaacgt gctcatgcgt ggccccgcgc tgcacgtgtt cgatggcgag ctcacgccgc
    13621 gtcaggtcgc gccgcgcccg taacgcgatc gctctcgcga agatccgggg tcaggcacta
    13681 cttctactac ttgccttccg gcttcgctgc atcggcgcgc tgacgttgct tgcttggtcg
    13741 cgtcctcggc ttcgtcgtgc cctggacgtg gatccacgga gctcgcgtcg agctcgcgtt
    13801 cgaccacgcg tcgacgattg cggtcgcgtc gcggtaccga cctgcccact gcatgctgga
    13861 tcagtacggc ttcggcgcgt tcgggcgacg agtcggcagt ttcgagccgg cagctacgtg
    13921 ctggatcagt tcggcttcgg cgcgttcggc gacgagtcgg ccggcttcgg tgcctcggtc
    13981 ggcttcgtcg tgtccgcggg cttcgtcgcg ggcgtggcgg gcttagtagc atccgccggc
    14041 ttcgtggcgt ctgcgggctt cgtggcgtct gcgggcttcg tcgccggcgt agagggcttc
    14101 gttgcatcgc cggccttcgt agcgtccgca ggcttggtcg ccggcgcagc gggcttcgct
    14161 gcgtcctgcg gcttcgtcgt cggcgcggtc gcaggcttcg ttgcgtcctg cggcttcgct
    14221 gcgggcggcg ttgccgtcgc gctgccagcg gcgggcgcgg cgctgcctgc cggaaccgtg
    14281 gcgctgccgg cggccggcgc tgcgctgcct gccggagccg tggcgctgcc cgtcggaggc
    14341 gtcggcgcgg cgctgcccgt tggcggcgtc ggcgtggtcg gctcgagcat catcgatgcg
    14401 ctgccagcgg aaccggccga gccggagcct tgctgcagtg ccttctcctt cgcttcttcc
    14461 ttctgcgtcg cgagctttgc ttgctcggaa ccgaacttct cgagcgcgtc gctcgagtcg
    14521 tggctcgcga tgaacgcgag gaccatcgac gtcgccatga acaccacggc tgcgcccgcc
    14581 gtcagcttct gcaagaacga cgacgcaccc gagccaccga acacggtgcc ggtgctgcta
    14641 ccgccgaacg caccacccat gccgccaccc ttaccggact gcagcagcac cgtgagcatc
    14701 aagaacaggc acacgatgac gtgcagaatc gtgatcagcg tcgacatgcg agcctagctt
    14761 ctaacccagc ggggtggccc gcgcaagctg gctaaacggg tcgtgatgcc ttcacgatcg
    14821 caacaaaatc agctgcttcg agcgaagcgc cgccaaccag tgcgccgtcg atatcggctt
    14881 ctgccatcag cgcctcggcg ttgctcggct tgaccgagcc gccgtactgg atgcgaatgg
    14941 cggcggcggt ctgtgcgccg aagcgctcgc ccaggcgctt gcggatgtgt gcgtgaactt
    15001 cttgggcctg cgccggcgtc gccgtgcgac ccgtgccgat cgcccagacg ggttcgtagg
    15061 cgatcacgag gcgctgcgcc gcaaccgcgg catcgatgcc ttcgagcccg cctgcgagct
    15121 gcgcatcgac cacgtcgagc gtgcgaccgc catcgcgctc ggcgagcatc tcaccgacgc
    15181 acacgatcac gccgagcccg cccgcgagca ccgcgcgcgc tttcttgccg accgactcgt
    15241 tcgtgtcgcc gaagaactgg cggcgttcgc tgtgcccgac gatcgaccac gtcgcgcccg
    15301 cgtcgctgag cagcggcgcc gagacttcgc ccgtgaacgc gccgctgttc tcccagaagc
    15361 agtcttgccc gcacgtcacg atcgccgagc cttcgagtcg cttggcgacc gcgttgatcg
    15421 cgaggaacgt cggcgcgacg ccgaccgcga cacccttgac cgcgccgagc tggttcttga
    15481 gctcggtgac gagcgcgagc gcttcgttga tcgtcttgtt cagcttccag ttgccgacca
    15541 cgaacggaat ccgcatgcgc gtggtgtacc gcgattggcg tccggatgtc cggcgggagc
    15601 cggacgtcgt gcacggcgcg cggacgaatt tgcgatgcgc gtcgaggaag aatcgcgacg
    15661 cgaacgcgct ccacgaagac acgcttcggc acactgaggc tatcggcgaa gctgtgttct
    15721 cgatttcgcc acgccggcgc cgctaggtcg accagctgag cgtgaactcg cactgctcgt
    15781 tgccggtaca gcggcaacgc ggatgcgtcg aggtgactcc ggccgcgccg gtcagctcga
    15841 tgatgcgctc gagctcggcg ctgacgagca ggcagacttc cgtcgagccg gcgaacccgg
    15901 cgagcgtgac gtccacatgg ttcggtttga ccccgacgac cacgccgccc cactcgtggt
    15961 agcggtccca gaacgtcggc aacgcagcca tcacggtctc cggcgatagc gacgagggat
    16021 cggcgccgaa caggcgcgcg aacgtggcgc tcatcgtgcc gcggccgacc ttgcgcgcga
    16081 cgagcgcgtt cggcagcgtg cgcttcgtga gatcgagcgc tgcgaccaga tcggcgagct
    16141 cgacccacgc gagtggcgcc agcgtcgagg cgagcacgtt cgcgagatcg ggacgctcgt
    16201 tcttcatctt cgcgatgccc gattcgccga tgtgatgctg gaggatcttc gacagcacgc
    16261 gcaggtgtgc ggcgcgcacg cgccccgaga cctcgggacg gatcgacgcc atcgggacga
    16321 cgccggatgc ttcgtcgatg cccttcgtcg ggccgagcac ggactcggcc tgcaacgcgg
    16381 caacgtcttg ctccgtcggc ggcacgcgca tctcgccgct catgcgctcg agcgcacgac
    16441 cgagtgcgac ggcgaacgtc gaggccgagg cccagcgctt cttcggattc ggctgtaacg
    16501 ctttgcggaa cacgacgtcg actgcttcgg agaggcctgc gcgcatttgc gacggcggca
    16561 tcatcggatc attgagctgg cgctggacga cctgcggcgc
//
LOCUS       DASUFX010000806        11036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1936430, whole genome shotgun sequence.
ACCESSION   DASUFX010000806 DASUFX010000000
VERSION     DASUFX010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936430"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2690
                     /locus_tag="VFV99_32645"
     CDS             <1..2690
                     /locus_tag="VFV99_32645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064184.1"
                     /translation="SCEHCRKIVAAAITGKTLAVGTPQPDVEDVLASAVDVSISGRYV
                     IEAVLGRGGMGTVYLARDQTLSRDVALKLHRAGSGNDRLHREAIAMAKLAHPNVVTVF
                     EIGSVDDRMYVAMEYVRGETLRGWLDASKRPWRTVIDMLAQAGAGLAAAHAAGLIHRD
                     FKPENVLVGEDGRPRVSDFGLARVGVGRDDKPTGVVTSMTQTGAILGTPAYMSPEQLA
                     GDPVDARCDQFAFCVVVWESLFGKRPFAGNTLAALEDAIQRQDIQRSARSDVPPRVRD
                     VIERGLAVNAADRYTDMQALLVALRAAAVPRTKKYLAVGLAAGVLLAAGGYAASSFVG
                     ARRHAAECDAAADNMRVLDPLQRENIRRAFIATGAPSANDTFERAAAVLDRYSGILAE
                     HARATCENTTEPLSLLAARQSCLADRRSEMAVLVDVISKPDASVVQRAPSTAWAIYDP
                     KPCSDRAVLLASSIVPVTRPREVVANLGRTRTLYDAGQYEEAARVAATVVDDARARKD
                     RGLELEALIEVASAKAELDPQAAVPIYEQAVQLAEALGRDLDAAGALGGLANLAGVSQ
                     NDYHAAHRYIALALAKLDRMGGDNLSLRGSLAATEAQVYSDENRLGEAETSIRQAAAA
                     LEQAYGPVHPKVGAVYGTLAQILRYQDKDSQPASERTVSIMRDTLGPEHPTTAGAEMT
                     LGQVLADKGHYDEALKLYAHADVVFAKSFGEHSLVRGSLFSNVATLELQRENWDAALT
                     GYRKALAIIEANEGPDAIDASGAHSDIARALASKGDMKAAFAEMDRSITIVEKAGSDG
                     DPRLIGRLTELAEYQIVAKQPAAGVATAERALAITKKRPADANPVEVGQLDFILARGL
                     WDSNGDRQRARTLVSEALELSQDSKERAMYQTWLAEHPL"
     gene            complement(2708..3187)
                     /locus_tag="VFV99_32650"
     CDS             complement(2708..3187)
                     /locus_tag="VFV99_32650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064185.1"
                     /translation="MASLSKEEARQLCERVAAYRKATAELPGFHKIRCGTDALGAAGA
                     RENTTPEAMRTSCTKELERCLASPPTIPEIDCTSSAGSGLVPGFMDQLARCPALTVDE
                     LFACTEEMRSMLTAIAQQDPCAMAFDPADTLASYNGYIRKLKGPHCAVMDERCKRTR"
     gene            3440..3865
                     /locus_tag="VFV99_32655"
     CDS             3440..3865
                     /locus_tag="VFV99_32655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064186.1"
                     /translation="MRWLVLFGLLAACDWFGDDGGSGAVCSYNGHDYSIGDVFPSGDG
                     CNSCSCTASGVACTARACADDGGVDADPAFCGATGGCPSGPACGAICCAMGEHCDNGM
                     CKCGSLPACGSGDSCEAPGPIGGDRCGSICCGSSGPCPQ"
     gene            complement(3866..5800)
                     /locus_tag="VFV99_32660"
     CDS             complement(3866..5800)
                     /locus_tag="VFV99_32660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5064187.1"
                     /translation="MRTQRLGRYELLRHLASGGMARVYLARATGPGGFTRHVVVKTIP
                     DERRDDDGFIAMFLDEARLVANLHHQHIAQVFDVDRTDDGTYFLAMEYVHGETVRHLL
                     QAATKRRAKLQLDVGLSIGCAAAAGLHHAHERRDHNGVPLGIVHRDVSPSNVILGYDG
                     SVKLIDFGIAKAKVRTTNTATGFIKGKAGYMAPEQARGYEVDRRSDVFALGVLVYELT
                     TQTRAFQADTHFETMHRTMQGQVEAPSKRVPDYPAALEDVVMTALELDPDDRFQDAES
                     MRVAFEQIARELGLALGDAPVMRALESLFPMRPEPWLAGAALSEVDDEDDDSMSEPLI
                     MFVPPTPTRQRLARGTEHEGAGEDNDAAERPLPQVVDVPELHATDLQAVPEIIPITDS
                     FELTTRPFERNTPATPQRAFVPVERARQEQETVPLPLVKRDRQETVSLKVRANGTGPE
                     SALTLPLPAAHETPLPKPPILDEPLDLPPPVRHNTPSVVAFSLPRAQSTTMSLAPRGQ
                     RRLLVVAGTAAATIIVAVIIAASTRTPTPAAVATTQATDAMPVPAPVAPPKPMPAPRA
                     EPATAPTSVTVHVTTDPDGATVVLDGVRLGVTPFTGSVPIKPGAGWLKVRKRKYAAVK
                     TRVSLERGVEWSVQLRRVEE"
     gene            complement(6027..6398)
                     /locus_tag="VFV99_32665"
     CDS             complement(6027..6398)
                     /locus_tag="VFV99_32665"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5064188.1"
                     /translation="MSSTQTYRGSCHCGAVTFTVTCEPITKGIRCNCSICRRRGLILS
                     DRWFTPEQISVAGTEALALYQFGDRYMNHWFCKTCGVYPFAEVNALSPTKAGQYRVNL
                     GCVDGVDPFALEIRLIDGASY"
     gene            complement(6395..6613)
                     /locus_tag="VFV99_32670"
     CDS             complement(6395..6613)
                     /locus_tag="VFV99_32670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064189.1"
                     /translation="MADAPPPGVGDPDCPHTERHEGVCVACGHCIHDVILNGACFLCG
                     TTDLDPIALSPKKPPSLVPADQLLRKKP"
     gene            complement(6615..7217)
                     /locus_tag="VFV99_32675"
     CDS             complement(6615..7217)
                     /locus_tag="VFV99_32675"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX5064190.1"
                     /translation="MADREIELFFDIGSSYSYLAATQMEGLAARSGVPVRWRPFLLGG
                     VFKATGNDMPARLPAKARWMMQDMALWAQHYGIAFRVPSRFPLITLRTQRALTAAERI
                     AGQRAIPSFALALFRAYWTEDQDITTDPVISAAARSAGLDADAIIAAIDAPETKDQLR
                     ATTDEAVRRGAFGAPAMFVGDALFWGNDRIALLEQYLARS"
     gene            complement(7233..>11036)
                     /locus_tag="VFV99_32680"
     CDS             complement(7233..>11036)
                     /locus_tag="VFV99_32680"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5064191.1"
                     /translation="QAVTRMAFASLLTHTRLRALAGYSYERGERYWREGRVQAYAPQG
                     DGVSGVVVGTSEYAVRVFAHGRTIAADCTCPVAGRDLFCKHAVALSLQYLEAPRDEDT
                     KPQRACFATRGELDDWCTEHQVTHELAIAADMLVEQITYSHPNLAMVLRNLSLRDVGS
                     LDGTGRYVGIRALQRPIAEAAAHWLERAAADVRVALGEEKRLRAVPSAEPLAGFVTSL
                     LGLRAQLRRKVAPRSRAMRSTGALDLDAKAGALVWREPTRISLRKQLWTTLTVSTRLV
                     FSPSPQLTCTCTLDRCVHALALVDAALDLLDDPADGARASLIAEELMRPPWQRALAEL
                     AAVDDAPATTRVEVWWQLEHQFGNRGIATLSPLVKKQLKKGGVGVGTRITPQRLLDEH
                     AAQLDEQDTRIAEALVGWVASRASTYPARAFTALVGHPRVVHEDEPDVLIAVKRLPLG
                     FAATATGEHIKLEPTVDGARFSPRLLAPLLDAFAPSEPLVVIEHEHARCLLIEVGDDA
                     RRLWAVLARHGDAFPPESHGALLEQLSRLEPRLPIDVPHTLKGAQLAANTTTVLRMRL
                     VRGATLEIEVFVRPAPGAPLFPPNAGPRDVMVQRDGARGYVRRQLGEEHAQVREALAR
                     LPLESAVEGPPLCFTIDDVDAALTLVAAVQTPPPGVEAEWIDARPTILSSGGPPQLRV
                     HIDTKRDWFGINGELVVEQGRLELAVLLDAMRRQQRFVRLDEERWVELSDALREHLTS
                     IADRTFAVRNQTELSPAAVPAVRALIEAGAQVDVAPAWQQLTERLAASTKLRPRPPTS
                     LATTLRPYQIEGHAWLSRLAAWGAGGCLADDMGLGKTIQAIAVMIDRAKLGPALVLAP
                     TSVTLNWIDELRRFGPTLRPVIYGEQADRAGCIAKLGKKDVLIVSYGLLVRDAAHLAS
                     RSFATLVVDEAQALKNPTTHRAKAARTLVADFRIALTGTPLENHLGELWSLFSIVFPN
                     LLGSWEQFRTRFAVPIERSHSADARAALSRLIKPFLLRRTKQEVARELPPRTEIHVPV
                     ALSSEETALYEDARLAAVAHLTRATKQTREERRRFDVLAALTRLRLLASHPKLYDSAS
                     TVASSKLQRLLELVEELREEGHRALVFSQFVSHLELVREELDAAGISSLYLDGSTPAA
                     TRKQLIDRFQTGEGDVFLISLKAGGTGINLTAADYVIHLDPWWNPAVEDQASDRAHRI
                     GQTKPVTVYRLISRGTVEERILAMHRDKRALVSSVLDGTGAAARLTMRDLLALVTDRA
                     EAHTA"
BASE COUNT         1790 a         3635 c         3819 g         1792 t
ORIGIN      
        1 acagctgcga gcactgccgc aagatcgtcg cggcagcgat cacgggcaag acgctcgcgg
       61 tcggtacgcc gcaacctgac gtcgaggatg tcctcgcgtc ggccgtcgat gtttcgatca
      121 gcggccgcta cgtgatcgag gccgtgctcg gtcgcggcgg catgggcact gtttatctcg
      181 cgcgcgatca aacgctgagc cgcgatgtcg cgctcaagct gcatcgcgcg ggctcgggca
      241 acgaccggct gcaccgcgaa gcgatcgcga tggcgaagct cgcgcatccg aacgtcgtca
      301 ccgtgttcga gatcggcagc gtcgacgatc gcatgtacgt cgcgatggag tacgtgcgcg
      361 gcgaaactct gcgcggctgg ctcgacgcga gcaagcgtcc gtggcgcacg gtgatcgaca
      421 tgctcgcgca agcaggcgca ggtctcgccg ccgcgcacgc cgccggactg atccatcgcg
      481 acttcaagcc cgagaacgtg ctcgtcggtg aagacggccg gccgcgcgtc agcgacttcg
      541 gtctcgcgcg cgtcggagtg ggtcgcgacg acaagccgac cggtgtcgtc acgtcgatga
      601 cgcagaccgg cgcgatcctc ggcacgccag cgtacatgtc cccggagcag ctcgccggcg
      661 accccgtcga cgcgcgctgc gatcaattcg cgttctgcgt cgtcgtgtgg gagagcctgt
      721 tcggcaagcg gccgttcgcg ggcaacacgc tcgcagcgct cgaagacgcg atccagcgtc
      781 aagacatcca gcgttcagcg cgcagcgacg taccaccgcg cgtgcgcgac gtgatcgaac
      841 gcgggctcgc ggtgaacgct gccgatcgct acaccgacat gcaggcgttg ctcgttgcgc
      901 tgcgtgccgc cgcggtgccg aggacaaaga agtatcttgc cgttggtctt gcagccggcg
      961 tgctgctcgc cgcgggtggg tatgcagcat cgtcgtttgt tggcgcacgc cggcacgccg
     1021 cggaatgcga cgcggccgcc gacaacatgc gcgtgctcga tccactccag cgcgagaaca
     1081 tccggcgcgc attcatcgcg accggcgcgc cgagcgcgaa tgacacgttc gagcgtgcgg
     1141 cggcggtcct cgatcgctac agcggcatcc ttgccgagca tgcgcgtgca acgtgcgaga
     1201 acacgaccga gccgctcagc ttgctcgctg cgcgtcagtc ctgtctggcc gatcggcgtt
     1261 ccgagatggc agtgctcgtc gacgtgatca gcaagcccga cgcgtcagtc gtgcagcgcg
     1321 cacccagtac ggcatgggcg atctacgatc cgaagccgtg tagtgatcgc gcggtgttgc
     1381 tcgcgagctc gatcgtgccc gtgacgcggc ctcgggaagt cgtggcgaac ctcggtcgca
     1441 cgcgcacgct ctacgatgct ggtcagtacg aggaagcggc acgtgtcgcg gccacggtgg
     1501 tggacgacgc acgcgcgcgc aaagatcgtg ggctcgagct cgaggcgttg atcgaggtcg
     1561 cgagcgccaa ggccgagctc gatccacagg ccgcggtgcc gatctacgag caagccgtcc
     1621 agctcgccga ggcactcggc cgcgatctcg atgcggcggg cgcgctcggc gggctcgcga
     1681 atctcgcggg cgtgagccag aacgactacc atgccgccca ccgttacatc gcgctcgcgc
     1741 tcgcgaagct cgatcgcatg ggcggcgaca acttgtcgct gcgtggctcg ctcgccgcga
     1801 ccgaagcgca ggtgtactcc gacgagaatc gcctgggcga ggcggagacc tcgatccgcc
     1861 aggcggccgc ggcgctcgag caggcgtatg gccccgtgca cccgaaagtc ggcgcggtct
     1921 atggcacgct cgctcagatc ctgcgctatc aggacaagga ttcgcagccc gcgagcgaac
     1981 gcacggtgtc gatcatgcgc gacacgctcg gccccgagca tccgacgacc gccggtgccg
     2041 agatgacgct cggccaggta ctcgcggaca agggacatta cgatgaagcg ctgaagctat
     2101 acgcgcacgc agacgtcgtc ttcgcgaagt cgttcggcga gcacagcctc gtgcgcgggt
     2161 cgctcttctc gaacgtcgcg acactcgagc tgcagcgcga gaactgggac gctgcgctca
     2221 ccggctatcg caaggctctc gcgatcatcg aggcgaacga aggtccggat gcgatcgacg
     2281 cctccggcgc gcacagcgac atcgcgcgcg cgctcgcgtc gaagggcgac atgaaggcgg
     2341 cgttcgcgga gatggaccgc tcgatcacga tcgtcgagaa agccggaagc gatggcgatc
     2401 cgcgcttgat cggacggctc accgagctcg cggagtatca gattgttgcg aagcaaccgg
     2461 ctgccggcgt cgcgaccgcc gagcgcgcgc tcgcgatcac gaagaagcga ccggccgatg
     2521 ccaaccctgt cgaggtcggt cagctcgatt tcatcctggc tcgtgggcta tgggacagca
     2581 acggcgacag gcaacgggcg cgaacgctgg tgtccgaagc gctcgagctg agccaggact
     2641 cgaaggaacg ggcgatgtac caaacgtggc tcgcggagca cccgctctaa ggtccgagcc
     2701 tcgaacctca tcgggtccgt ttgcaccgtt cgtccatgac cgcacagtgc ggtcctttca
     2761 gctttctgat gtagccgttg tagctcgcga gagtatctgc ggggtcgaac gccattgcgc
     2821 atgggtcttg ctgcgcgatg gcggtcaaca tgctccgcat ctcctcggtg caagcgaaca
     2881 gctcgtcgac cgtaagcgcc ggacagcgtg ccagctgatc catgaacccg ggcacgagcc
     2941 ccgaccccgc ggacgacgtg caatcgatct cgggaatcgt cggcgggctc gccaggcatc
     3001 gttcgagctc tttcgtgcag gaggttcgca tcgcctcggg cgtcgtgttc tcccgggccc
     3061 ctgcagcgcc cagcgcatcc gtgccacacc ggatcttgtg aaaacccggc agctcggcgg
     3121 tcgcttttcg gtacgccgcg actcgctcgc aaagctgcct agcttcctcc ttcgacaggc
     3181 tggccaccgt cttcgagtgc gccgacgacg aagcccggtc cgacgacgag cacccgacta
     3241 cgaggacgat ggcgatccac tcaacgcgca tgccgagatg atcctagagg gtcccgtaga
     3301 agacgagagc tgcgagcagg gcaacaagga gcggttcgta gaagcgatcg aagcgggtca
     3361 cagacatacg agccccgcgc gtgacgccgg tcgcgcgaaa accgcgatcc gcggcgcaac
     3421 cccgcgatac gatggaggca tgaggtggct ggtgctcttc ggcctgctcg cggcgtgcga
     3481 ttggttcggt gacgacggtg gtagcggcgc ggtgtgcagc tacaacggcc acgactattc
     3541 gatcggcgat gtgttcccgt ccggtgatgg ctgcaactcg tgctcgtgca ccgcgagtgg
     3601 tgtcgcctgc acggcccgcg cgtgtgccga tgatggcggc gtcgacgccg accccgcatt
     3661 ctgcggcgcc accggtggct gcccttcggg tcctgcatgc ggcgcgatct gttgcgcgat
     3721 gggcgaacat tgtgacaacg gcatgtgcaa gtgcggctcg ttgccggcgt gcggcagcgg
     3781 cgacagttgc gaagcgccgg gcccgatcgg tggcgatcgg tgcggctcga tctgttgtgg
     3841 ctcgagcggg ccgtgtccgc agtagctact cttcgacgcg gcgcagctgc acgctccact
     3901 cgaccccgcg ctcgagcgac acgcgggtct tcaccgcggc gtatttgcgc ttgcggacct
     3961 tgagccaccc ggcgcccggc ttgatcggca cggagccggt gaacggcgtc acgccgagcc
     4021 gcacgccatc gaggacgacg gtggcgccgt cgggatcggt cgtgacgtga acggtgacgc
     4081 tcgtcggtgc ggtggcgggt tccgcgcgcg gcgccggcat cggcttcggc ggcgcgaccg
     4141 gcgccggcac gggcatcgca tcggtcgctt gcgtggtcgc gaccgcggcg ggcgttggtg
     4201 tgcgcgtcga ggccgctatg atgactgcaa cgataatcgt ggcggccgcc gtaccggcaa
     4261 cgaccagcaa gcggcgctga ccgcgcggcg cgaggctcat cgtcgtgctc tgggcacgcg
     4321 gcagtgaaaa cgcgaccacc gatggcgtgt tgtggcgcac cggcggcggc aggtcgagcg
     4381 gctcgtcgag gatcggcggc ttcgggagtg gcgtctcgtg cgctgccggc agcggcaacg
     4441 tcaatgccga ctcgggcccg gtgccattcg cgcgaacctt gagcgacacc gtctcttggc
     4501 ggtcgcgctt tacgagcggc agcggcacgg tctcttgttc ttgccgtgcg cgctcgacag
     4561 ggacgaacgc gcgctgcggc gtcgccggcg tgttccgctc gaacggccgg gtcgtcagct
     4621 cgaaactgtc ggtgatcgga atgatctccg gaacggcctg aagatccgtc gcatggagct
     4681 cggggacatc gacgacctgc ggcagcggtc gctcggccgc gtcgttgtcc tcgccggcgc
     4741 cttcgtgttc ggtgccgcgc gcgagccgtt ggcgcgtcgg tgtcggcggc acgaacatga
     4801 tcagcggctc gctcatcgag tcgtcgtcct cgtcgtcgac ttccgacagc gcggcaccgg
     4861 cgagccacgg ctcggggcgc atcgggaaca agctctcgag cgcgcgcatc accggcgcat
     4921 caccgagtgc gaggccgagc tcgcgcgcga tttgctcgaa cgcgacgcgc atcgattcgg
     4981 catcctggaa tcgatcgtcg ggatcgagct cgagggccgt catgaccacg tcctcgagcg
     5041 cagccggata atcgggcacc cgcttcgacg gcgcctcgac ctggccttgc atcgtgcggt
     5101 gcatcgtctc gaagtgggta tcggcctgaa acgcgcgggt ctgggtcgtc agctcgtaga
     5161 cgagcacacc gagcgcgaac acgtcgctgc ggcgatcgac ctcgtagcca cgcgcctgct
     5221 cgggcgccat gtagccggcc ttgcctttga tgaagccggt cgccgtattc gtcgtgcgga
     5281 ccttcgcctt cgcgattccg aagtcgatca gcttgacgga gccgtcgtag cccagaatca
     5341 cgtttgacgg cgagacgtcg cgatggacga tgccgagcgg tacaccgttg tgatcgcgtc
     5401 gctcgtgcgc gtgatggagg cccgctgctg ccgcgcagcc gatcgacaat ccgacgtcga
     5461 gctgcagctt cgcgcgccgc tttgtcgcgg cctgcaagag gtgccgcacg gtctcgccgt
     5521 gcacgtactc catcgcgagg aagtacgtgc cgtcgtcggt gcggtcgaca tcgaagacct
     5581 gcgcgatgtg ctggtggtgg aggttcgcga ccagccgcgc ctcgtcgaga aacatcgcga
     5641 tgaatccgtc atcgtcgcgt cgctcgtccg ggatcgtctt gacgaccacg tgacgcgtga
     5701 atccgcccgg ccccgtagca cgcgccaagt acacccgagc catccctccc gatgccaggt
     5761 gacgcaacag ctcgtacctg ccgaggcgct gggtacgcat gctgcgagaa ggtcgtagcc
     5821 cagcgagcgt acgtcaagaa agcaatgcgt gattctcgcc acgatggcga gcaccgctca
     5881 tcaacgcagt aatctggcgc acgatgtgcg tgatcgcgtt tgcacgcgcg tggtggtcgg
     5941 acgttatcgc gccaatcgtg gcgcggctgt tactggcgac ggctcactaa cggacccggt
     6001 ttgatccgat ttgatctgca catcgatcaa tacgacgcac cgtcgatcaa acggatctcg
     6061 agggcaaacg ggtcgacgcc gtcgacgcag cccaaattga ctcgatactg gcctgccttt
     6121 gtcggactga gcgcgttgac ctctgcgaac gggtagacgc cgcacgtctt gcagaaccaa
     6181 tgattcatgt agcgatcgcc aaactgatag agcgcgagcg cctcggttcc ggcaacagag
     6241 atttgctcgg gcgtgaacca gcggtccgac aggatcaggc cccggcggcg gcagatcgaa
     6301 caattgcacc ggatgccctt cgtgatcggt tcgcaggtga ccgtgaaggt caccgcgccg
     6361 cagtggcaac tgccgcgata tgtctgcgtc gagctcatgg tttctttcgc agtaattgat
     6421 cggcagggac gagcgatggc ggcttcttcg gtgacaacgc gatcggatcg agatcggtcg
     6481 tgccgcacag aaagcaggcg ccgttgagaa tcacgtcgtg aatgcagtgg ccgcacgcga
     6541 cgcacacgcc ttcgtggcgt tcggtgtgcg ggcaatcggg atcgcccacg ccaggtggcg
     6601 gcgcatcagc cacgtcagct ccgcgcgagg tactgctcga ggagcgcgat gcgatcgttg
     6661 ccccagaaca gcgcatcgcc gacgaacatc gcaggcgcgc cgaatgcgcc gcggcgcacg
     6721 gcctcgtcgg tcgtagcgcg cagctggtct ttggtctccg gcgcgtcgat cgcggcgatg
     6781 atcgcatcgg cgtcgaggcc ggcactgcgc gcagcggcgc tgatcaccgg gtcggtcgtg
     6841 atgtcctggt cctcggtcca gtacgcgcgg aacagcgcga gcgcgaacga cgggatcgcg
     6901 cgttgaccgg cgatgcgttc ggcggcggtg agcgcgcgct gggtgcgcag cgtgatcagc
     6961 gggaagcggc tcggtacgcg gaacgcaatg ccgtagtgct gcgcccacag cgccatgtct
     7021 tgcatcatcc agcgcgcctt cgcgggcagc ctcgcgggca tgtcattgcc ggttgctttg
     7081 aagacaccgc cgagcaagaa cggccgccag cgcacgggca cgccggatcg cgcagcgagc
     7141 ccttccatct gggtcgctgc gagatagctg tacgaagaac cgatatcgaa gaagagctcg
     7201 atctcgcgat ctgccatggc gtgaccctag cactacgcag tgtgggcttc cgctcgatcc
     7261 gtgacgagtg caagcagatc gcgcatggtc agccgggcag ctgcgccggt accgtcgagc
     7321 acgctcgata cgagcgcgcg cttgtcgcga tgcatcgcga ggatgcgctc ttcgacggtg
     7381 ccgcgcgaga tcaagcgata gacggtaacg ggcttcgtct ggccgatgcg atgcgcgcgg
     7441 tcggacgcct gatcttcgac ggccgggttc caccacggat cgaggtggat gacgtaatcc
     7501 gcggcggtga ggttgatgcc ggtgccgcca gccttgagcg agatcaagaa cacgtcgcct
     7561 tcgccggtct gaaaccgatc gatgagctgc ttgcgcgtgg ccgcaggcgt actgccgtcg
     7621 agatagagcg acgagatgcc ggccgcgtcg agctcctcgc gcacgagctc gaggtgcgac
     7681 acgaactggc tgaacacgag cgcgcgatga ccttcctcgc gcagctcctc gacgagctcg
     7741 agcaggcgct gcagcttcga cgacgcgacc gtcgaggccg agtcatacag cttgggatgc
     7801 gatgcgagca ggcgcaggcg cgtcagtgcg gcgagcacgt cgaagcgccg gcgctcctcg
     7861 cgcgtctgct tcgtcgcgcg cgtgaggtgg gctactgcag cgagtctcgc gtcctcgtag
     7921 agcgcggttt cctcgctcga cagcgcgacc ggcacgtgga tctcggtgcg tggcggcagc
     7981 tcgcgcgcga cctcttgctt ggtgcggcgg agcaagaatg gtttgatcaa gcgcgacaat
     8041 gccgcgcgcg cgtcggcgct gtggctgcgt tcgatcggca cggcgaaccg cgtgcggaac
     8101 tgctcccagc tgccgagcag attcgggaac acgatcgaga acaagctcca cagctcgccg
     8161 agatgattct cgagcggcgt gccggtcagc gcgatgcgga agtcggcgac gagcgtgcgc
     8221 gcggccttcg cgcggtgcgt ggtcgggttc ttgagcgcct gcgcttcgtc gacgacgaga
     8281 gtcgcgaacg agcgcgaggc gaggtgcgcg gcatcgcgga cgagcaggcc gtagctgacg
     8341 atcagcacgt ccttcttgcc gagcttcgcg atgcagccgg cgcggtcggc ttgctcgccg
     8401 tagatcacgg gccgtagcgt ggggccgaac cggcgcagct cgtcgatcca gttgagcgtc
     8461 actgacgtcg gcgcgagcac gagcgcgggc ccgagcttcg cgcgatcgat catcaccgcg
     8521 atcgcctgga tcgtcttgcc gaggcccatg tcgtcggcga gacaaccgcc cgcgccccac
     8581 gccgcaagtc ggctcagcca ggcatggcct tcgatctggt atggacggag cgtggtggcg
     8641 agcgacgtcg gtggcctcgg cctcagcttc gtcgacgccg cgagtcgctc ggtcagctgt
     8701 tgccatgccg gcgcgacgtc gacctgcgcg ccagcctcga tgagcgcgcg cactgcgggc
     8761 accgccgccg gcgacaactc ggtctggttg cgcacggcga acgtgcgatc ggcgatgctg
     8821 gtcaggtgct cgcgcaacgc gtccgacagc tcgacccagc gctcctcgtc gagccggacg
     8881 aatcgctgct ggcggcgcat cgcatcgagc aatacggcca gctcgagccg gccttgctcg
     8941 acgacgagct cgccgttgat cccgaaccag tcgcgtttgg tatcgatgtg tacgcgcagc
     9001 tgcggcggac cgccggagct caggatcgtg ggccgcgcat cgatccattc ggcctcgacg
     9061 ccaggcggcg gcgtctgtac cgccgcgaca agcgtgagcg ccgcgtcgac atcatcgatc
     9121 gtgaagcaca gcggtgggcc ctcgacagcg gactcgagcg gcagccgagc gagcgcctcg
     9181 cgcacctgtg cgtgctcttc gcctaactgt cgacgcacgt agccgcgggc gccgtcgcgt
     9241 tgcaccatca catcgcgcgg accggcgttc ggcgggaaca gcggggcacc gggcgcaggc
     9301 cgcacgaaca cctcgatctc gagcgtcgcg ccccgcacga gccgcatgcg caacaccgtc
     9361 gtcgtgtttg cggcaagctg ggcgcccttg agcgtgtgcg ggacgtcgat cggcagacgc
     9421 ggctcgagcc gcgacagctg ctcgagtagc gcaccgtggc tctccggtgg aaacgcatcg
     9481 ccgtgcctgg cgagcaccgc ccacagccgg cgcgcgtcgt cgccgacctc gatcagcagg
     9541 cagcgagcgt gctcgtgttc gatgacgact aacggctcgg acggagcgaa tgcgtcgagc
     9601 aacggcgcga gcaggcgcgg actgaatcgc gcaccgtcga ccgtgggctc gagcttgatg
     9661 tgctcgccgg tcgcggtcgc tgcgaaaccg agtggtagcc gcttgaccgc gatcaggacg
     9721 tcgggttcgt cctcgtgcac gacgcgcgga tggcccacaa gcgcagtgaa cgcgcgcgca
     9781 ggatacgtgc tcgcgcgcga cgcgacccag ccgacaagcg cctccgcaat gcgcgtgtcc
     9841 tgctcgtcga gctgcgcggc gtgctcgtcg agcagacgtt gcggcgtgat gcgcgtgccc
     9901 acgccgacac cgcctttctt gagctgcttc ttgaccagcg ggctgagcgt cgcgatcccg
     9961 cggttgccga actgatgctc tagctgccac cacacctcga cgcgagtggt cgctggtgca
    10021 tcgtcgacgg cagcgagctc ggcgagcgcg cgttgccagg gcgggcgcat gagctcttcg
    10081 gcgatcaggc tggcgcgcgc tccatccgcc ggatcgtcga gtagatcgag cgccgcgtcg
    10141 acaagcgcga gcgcgtgcac gcacctgtcg agcgtgcacg tgcacgtcag ctgaggggat
    10201 ggcgagaaca cgagccgggt gctgaccgtg agcgtcgtcc atagttgctt gcgcagcgag
    10261 atccgcgtcg gctcccgcca gacgagtgcg ccggccttcg cgtcgagatc gagcgcgccg
    10321 gtcgaccgca tcgcgcgcga gcgcggcgcg accttccggc gcagctgtgc gcgcaggccg
    10381 agcaagctcg tgacgaaccc ggcgagtggc tcggcggacg gaacagcgcg caaccgcttc
    10441 tcttcgccga gcgcgacgcg aacatccgca gcggcacgct cgagccagtg cgccgctgcc
    10501 tccgcgatcg gccgctgcag cgcgcgaatg ccgacgtagc ggcccgtacc gtcgagcgag
    10561 ccgacatcgc gtagcgacag gttgcgcagc accattgcga ggttcggatg tgagtacgtg
    10621 atctgctcga cgagcatgtc ggccgcaatc gcgagctcgt gcgtgacctg gtgctcggtg
    10681 caccagtcgt cgagctcgcc gcgcgtcgca aagcatgctc gctgtggctt cgtgtcttcg
    10741 tcccgcggcg cctcgaggta ttgcagcgag agcgcgacgg catgcttgca aaacaggtcg
    10801 cggccggcga ccgggcacgt gcaatccgcg gcgatcgtgc gcccgtgcgc gaacacccgc
    10861 accgcgtact cgctggtccc gacgacgacg ccggacacgc catcaccctg cggtgcgtac
    10921 gcttgcaccc ggccttcgcg ccagtagcgc tcgccgcgct cgtagctgta cccggccagc
    10981 gcgcgcaggc gggtatgggt caacagcgac gcgaacgcca tccgggtcac agcttg
//
LOCUS       DASUFX010000807        12742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1382157, whole genome shotgun sequence.
ACCESSION   DASUFX010000807 DASUFX010000000
VERSION     DASUFX010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382157"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2222)
                     /locus_tag="VFV99_32685"
     CDS             complement(<1..2222)
                     /locus_tag="VFV99_32685"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5064192.1"
                     /translation="MTRSTDGQDVLEQNVSTLLESGGEPPMISDVARARIRNALVANH
                     GVAEVATKARVRVPLLAVGIGIAATAATALLVSHFVGGSSAVDSHKLADGSTFVTKDG
                     GEVVVLGPRHVRVKGEALLDVAPGKGTFLVETKAGRIEVLGTRFVVDAQPAKTMTAVV
                     RGEVKLATDQGAVVLHAGEQAVAEPGRPPTRGPAPRLSHLVSWAQTARHREENGIEPA
                     RHGTLFARDPGVRPNGQFGEEYPLPLAKLALDVVVEDQVARVALDQTFHNPQARVLEG
                     VYKFAIPPDASLQRLAMYVDGKLTESAVVERMRARRIYEELVYRRIDPALLEYAGTGR
                     LNLRVYPLPANQDKRLMVAYTQSVPKLYTDYTLSIPLPEVDQPVGDMLVSARIKGCAN
                     CELSSPSHKIEVERSGDDAVVKYHKNGEKIGDSFVVHVRDTRKQTTVAEMVDGKDHYM
                     LVRAPVQLGSMTRGQREYRPRTWVILDDVSASRSALELKAQADLVDAFLRELDENDKV
                     ALVAFDVEARQKLAPTRVIDVDRRVVRQALKDEGGVGATDFDKALAAAMRLLDGTNPD
                     DAMIVYLGDGVITSGTRQLDTLRAQIAGKAHFVGVGVGDGPDTQTLEALAAATGGYSA
                     TFDLADDLGWRAFDLIAALHTSRVTGVTAKLVDAAGQLVPATAYLPSAQLADGEQLQL
                     VAKLAGDGTPKAVQLSGTRGGAPWQQTIELSTGHGNAAYLPRLWAQQHIAARLLAKHE
                     P"
     gene            complement(2219..2845)
                     /locus_tag="VFV99_32690"
     CDS             complement(2219..2845)
                     /locus_tag="VFV99_32690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5064193.1"
                     /translation="MIAALFARLFRSGPGGDLRAAQDGDRLAIGRFYDAHVDGLYAFV
                     FYRVGRDAALAEDVVQETFTLALGKRDAYDPARGSVASWLTVLSRNVIRDHLREHRRA
                     DALQATWERIDATLAQTFAAMAERPLPGDVLERAETRDLVHMAVANLPEQYRTVLTRK
                     YVDGESLETLARDLGISVDAAKSLLARARRAFRDTFATLSAHFVEATP"
     gene            2962..4086
                     /locus_tag="VFV99_32695"
     CDS             2962..4086
                     /locus_tag="VFV99_32695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064194.1"
                     /translation="MRILPTALAISAVVHGVGIVWVRSRPVHKPEPTKLVTIETIEVV
                     PAPAQRGEPVPTEVTLLDDNSVAATAPNASSRGNTKPGKSKQHLSASATGTTETAPAT
                     NETPPPRSSLMTMRHPDRPKLENGPSAAFWEKFEANTKPLQPKEIAGEQRNDEIATAE
                     GNLSNQRWIDNATSEEVGGERAKLIQKRYERSTAELQPDGQGTKSDHRTFKAKFNPDG
                     TVASIDDKANLRRKGLGAEFDVTDAMMRAKGIDPYSSYKLKVLDDTREERVAIGKRYR
                     TQQLAQSKQLMQKNLERLWAMTHDLTARKAGLFELWDDCAETGSDELVAGGASARAHV
                     VGFIRSKLPEGSADAFTTADLERFNKQRKSREQFAPYQTL"
     gene            4096..4545
                     /locus_tag="VFV99_32700"
     CDS             4096..4545
                     /locus_tag="VFV99_32700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064195.1"
                     /translation="MRAARLVVVLAVLWSVPAHADHGKFGLGLIVGQPTGLTGAYDLS
                     EKSQIDAALGLALFDDRDFYIHVEYLYYPFTLVHGGDLDLSAYLGIGGWIVTVHDPVI
                     GARAPFGLSLDFNTAPIQIFLEASVLVSIVPDAHGAVRGAAGFRYYF"
     gene            4567..5676
                     /locus_tag="VFV99_32705"
     CDS             4567..5676
                     /locus_tag="VFV99_32705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064196.1"
                     /translation="MGFDGPLIAGRYRLGPRLGHGSQGEIFLARDEKAKGTDDKDVVV
                     KRMTPRGTWKSFELFERETKVLGQLRHPGVPRYLTTVEEPPGTFNLVMQRMPGENLRD
                     FAKRRRLSETELRDVLIRCLEILDYLHTRTPPVVHRDIKPSNIMRAPIDGRCALVDFG
                     GVLDAAREKGGSTIVGTFGYMAPEQLHGQAVPATDIYALGATMVALAGGVEPEDVPRK
                     GLKMDLDKHLPNLDPTFREALIAMTDPDPDHRPQRAREVVALLAKARPPKRRDEKALV
                     AQGTKEVAPRRMFSDVQEPLGTLLRIGVLGFGFGGWVGMASIRVSLMLTIYILAALGF
                     PKRRRVLEVGHELDAMLAEGQGGFTDMMKGAMARR"
     gene            5673..6425
                     /locus_tag="VFV99_32710"
     CDS             5673..6425
                     /locus_tag="VFV99_32710"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5064197.1"
                     /translation="MTTKLITIPFSHYCEKARWALDRCGIAYEERGHLPLFHYLDSYR
                     AARQRTVPILIDGDTLVKDSTDIIAWADAKRPGTLIPVAGAENVLAIEDDLDNHFGPH
                     TRRWGYWYLLPNRAALEYIKVGVPRWEASLLGISRPLAAAFLRRGLNINAESVERSRK
                     KIDATFDRIEQLLGDGRRYLAGDRFTVADLTFASLAAPVLVPPNHPFQTFDLDLFPAE
                     AQDQVAAWRARPAGKLALRLYADDRAVLPKAA"
     gene            6576..8222
                     /locus_tag="VFV99_32715"
     CDS             6576..8222
                     /locus_tag="VFV99_32715"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064198.1"
                     /translation="MMRLLAAALLVSVIGCSSSEEGKADTPPTLEVTSPARGTFADAD
                     TVTVSGRATDKEGPVKVTVGGMAVTPAADGTFTATVPVTPGVDLIETHAIDSAGTDVR
                     DVRAVLAGTLGPTDGTAKGHVGARAGVTALRAVGNAVAATAKTIDFTAAAQALNPVYN
                     NTGCLGAKIDITSISLTNVGVNLAPQTNVLATDVTIDNLDVRLHADFKVACIGGSTTI
                     TIRSSKAHVHGDLAAAITAGKITTSLPATAVTLDNFSIDVGGVPGAIESLLKDQARSG
                     VEKALTSAIKSKVPPIADKALAGLIAKPVTASLLGHDMTIGITPTKVSITPSELFVTV
                     DTTLRVAGGEGGTFLTTDMALTSATMGSSQGLGIALDDDIVNQLFAGLWAADAFDYTV
                     SIDTIPSLGAVLDDDARSVELKLSLPPTASTDSGELELAIGDLMISVKDDAGAELQSI
                     AVSITTTVSAEPSQTGKVLLTVGAPTVYAQVLAQTAAVDDPLTDQQVEALISGVWGLI
                     GVKADDALSKLPMPTIAGISLGAPTVTTSTGYVLADMSVN"
     gene            complement(8458..8889)
                     /locus_tag="VFV99_32720"
     CDS             complement(8458..8889)
                     /locus_tag="VFV99_32720"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5064199.1"
                     /translation="MRWTDADGASWSLHEACYAGKLDAVKALLDMGADPNGNAADDDA
                     WEWISAAGPHPRPLHCAAIAWEHLPDHAEIVKLLVARGAKIEESVLQDYWIETTLTDN
                     AIAVGVALGEDEADLRGLREKHGAVEDRYDNGKVLFVKPSD"
     gene            complement(8904..9056)
                     /locus_tag="VFV99_32725"
     CDS             complement(8904..9056)
                     /locus_tag="VFV99_32725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064200.1"
                     /translation="MEEAIAVICDAEQRVLADAALIGDAELRRSFVEAVPDHAQTLSL
                     ARAWRS"
     gene            complement(9107..9856)
                     /locus_tag="VFV99_32730"
     CDS             complement(9107..9856)
                     /locus_tag="VFV99_32730"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5064201.1"
                     /translation="MPPPPPRVTEHEVRVKDLDPRHDGVRIAQLSDIHVGNMTPASHV
                     RAAVEAANGAKPDIIVLTGDYVCWRRHEAEMAGDQLSGLTASRVLAVLGNHDYFTWGR
                     GVASALEKNGYEVLKNQHTVAQINGAPLALVGVDDPVTRHDDRDAAFKGTHEKHTKVV
                     LCHAPDAAPRLAERGADLVLSGHTHGGQIYIKGITDRLMKKIGLHYRRGHFAFGKTTL
                     YVTPGVGFSGVTRRHGEGTDAEVAVLTLRAA"
     gene            9982..10893
                     /locus_tag="VFV99_32735"
     CDS             9982..10893
                     /locus_tag="VFV99_32735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064202.1"
                     /translation="MAAVRPPGIADARVPCPLCGGLIHPVAGRCKHCKEDLSSFREGR
                     PQAAAALPALNGKPAQTGGMANGSNGHAAATAIPAAIPTHEASQPILPPRTTARSMQA
                     QRPSHSIWRSWPMLVIVLAVIAIVAATVIMILPPGSKKHDGKMSAPPGPAFERMETNP
                     LPDKSSQLDPWDNGAQGATPRAPDRQPQPQAPTPVDPDDDLWNGSGGIAGGGSFGMTG
                     NAFMIHVLDRACKKLKTCPDLDQSSLSTICESVAMMPKPPAPTCAAAQKCFDAIDRLS
                     CSQASAQDMSPVSIFGLFSDCRDADKC"
     gene            10906..11772
                     /gene="panC"
                     /locus_tag="VFV99_32740"
     CDS             10906..11772
                     /gene="panC"
                     /locus_tag="VFV99_32740"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5064203.1"
                     /translation="MTYLRPEFEIVRDPAAMRARAEDLRRDGRRIAVVPTMGALHEGH
                     LTLLRAARAKCDILILTIFVNPTQFGPKEDLAKYPRDEAGDLAKARACGIDLAFCPDV
                     TAMYPTGAQTFIEVRELQKPMCGASRPGHFAGVATVVAKLFNITKPHLAVFGEKDFQQ
                     LAIIRRMVRDLDFGIEIVGVPIVREADGLALSSRNAYLSADQRRAALSLNKGLAAAEA
                     AFKAGERNVGTLLAAARAPIDAESTMRIDYLELRDADELTPIDTVARRSVLAVAAFAG
                     TTRLIDNRVLAP"
     gene            11994..12554
                     /locus_tag="VFV99_32745"
     CDS             11994..12554
                     /locus_tag="VFV99_32745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064204.1"
                     /translation="MLGLTSTTAFAQENAAPATPPAPTTAAASLETKPAQNALYAELG
                     GNSGWYSINYERFIRPDAAIRVGLSYMSVTATAGSGENSASANATWATLPVMFEYLGI
                     HAGSHALELGGGVNFMYFSGSAATFDATAMSTGIVPVGTATIGYRYSNPDGGFIFRAG
                     YTPLIFVSTEQKDIFHWGGMSFGYRF"
     gene            complement(12542..>12742)
                     /locus_tag="VFV99_32750"
     CDS             complement(12542..>12742)
                     /locus_tag="VFV99_32750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064205.1"
                     /translation="NIGNAQPVPLTKPAPPADDEPEIEVLPTLDAVAAEPAASSDGGE
                     EPSGKQGWVSSLLNRLSRTQKR"
BASE COUNT         2157 a         4384 c         4107 g         2094 t
ORIGIN      
        1 ggctcgtgct tcgcgagcag gcgcgcagcg atgtgctgct gcgcccacag ccgcggcaag
       61 tacgccgcat tgccgtgacc ggtggagagc tcgatcgtct gttgccacgg cgcaccgccg
      121 cgcgtgccgg agagctgcac cgccttcggg gtgccatcgc cggccagttt cgcgacgagc
      181 tggagctgtt caccgtcggc gagctgcgcc gatggcaaat acgcggtcgc cggcacgagc
      241 tggcccgccg catcgacgag cttcgcggtc acgccggtta ctcgcgacgt gtgcagcgca
      301 gcgatcagat cgaacgcgcg ccagccgagg tcatccgcaa gatcgaacgt cgccgagtaa
      361 ccaccggtcg ctgccgcgag tgcttcgagc gtctgcgtat ccggtccatc accgacgcca
      421 acgccgacga agtgcgcctt gcctgcgatc tgcgcgcgca acgtgtcgag ctggcgagtg
      481 ccgctcgtga tcacgccgtc accgagatac acgatcatcg cgtcgtcggg gttggtgccg
      541 tcgagcagcc gcatcgcggc cgcgagtgct ttgtcaaagt ccgtcgcacc gacgccaccc
      601 tcgtctttga gcgcctgccg cacgacgcgg cgatcgacat cgatcacacg cgtcggcgcg
      661 agcttctggc gcgcttcgac atcgaacgcg accagcgcaa ccttgtcgtt ctcgtcgagc
      721 tcgcggagga acgcatccac gaggtcggcc tgcgccttga gctcgagcgc actgcgcgat
      781 gcgctgacgt cgtcgaggat cacccacgtg cgcgggcgat actcgcgttg cccccgtgtc
      841 atagacccca gctggaccgg cgcgcgaacc agcatgtagt ggtccttgcc gtcgaccatc
      901 tccgcgaccg tcgtctgctt gcgcgtgtcg cgcacgtgga cgacgaacga gtcgccgatc
      961 ttctcgccgt tcttgtgata cttgacgacc gcgtcgtcgc cgctgcgctc gacctcgatc
     1021 ttgtggctcg gcgacgacag ctcgcagttc gcgcagccct tgatgcgcgc gctcaccagc
     1081 atgtccccga ccggctgatc gacttccggc agcggaatcg acagcgtgta gtcggtgtac
     1141 agcttcggaa cgctctgcgt gtacgcgacc atcagccgct tgtcctgatt tgcgggcagt
     1201 gggtacacgc gcaagttgag tcggccggtg cccgcgtatt cgagcagcgc cggatcgatg
     1261 cgccggtaca cgagctcctc gtagatccgg cgcgcacgca tgcgctcgac gaccgccgac
     1321 tcggtcagct tgccgtcgac gtacatcgcg agtcgctgca acgacgcgtc gggtggaatt
     1381 gcaaacttgt agacgccctc gagcacgcgc gcctgcgggt tgtggaacgt ctgatcgagc
     1441 gcgacgcgcg cgacctgatc ttcgacgacg acatcgagcg ccagcttcgc gagcggcagc
     1501 ggatactcct cgccgaactg accgttcggg cgcacgcccg gatcgcgggc gaacagcgtg
     1561 ccgtggcgcg ccggctcgat gccgttctcc tcgcgatggc gcgcggtctg cgcccagctc
     1621 acgagatgcg acaggcgcgg cgccggtccg cgcgtcggcg gccgacctgg ctcggcaacc
     1681 gcttgctcgc cagcgtgcag cacgaccgcg ccttgatcgg tcgcgagctt cacctcgccc
     1741 cgtacgactg cggtcattgt cttggccggc tgagcgtcga cgacgaagcg cgtgccgagc
     1801 acttcgatgc gacctgcctt ggtctcgacg aggaacgtgc ccttgccggg cgcgacatcg
     1861 agcagcgcct cgcccttcac ccgcacgtga cgcgggccga gcacgacgac ctcgccgccg
     1921 tccttcgtca cgaacgtgct gccgtcggcg agcttgtgcg aatcgaccgc cgagctgccg
     1981 cccacaaagt ggctgacgag cagcgcggtt gctgccgtcg ctgcgatccc gataccgacc
     2041 gcgagcagcg gcacgcgcac gcgcgccttc gtcgcgacct ccgcgacgcc gtggttagca
     2101 acgagcgcgt tgcgaatccg cgcgcgcgcg acatcggaaa tcatcggcgg ctcgccgccg
     2161 ctctcgagta gcgtcgaaac gttttgctcg aggacgtcct ggccatctgt actgcgagtc
     2221 atggcgttgc ctccacgaag tgcgccgaca gcgtcgcgaa cgtgtcgcgg aacgcgcgtc
     2281 gcgcgcgggc gagaagagat ttggctgcgt cgaccgagat gccgagatcg cgtgcgagcg
     2341 tctcgaggct ctcgccatcg acatacttgc gggtgagtac ggtgcggtac tgctcgggga
     2401 gattcgcaac cgccatgtgc acgaggtcgc gcgtctcggc gcgctcgagc acatcaccgg
     2461 gcaacggccg ctccgccatt gccgcaaacg tttgcgcgag cgtcgcgtcg atccgctccc
     2521 acgtcgcctg cagtgcatcc gcgcggcgat gctcgcggag atgatcgcga atcacgttac
     2581 gcgacagcac ggtgagccag ctcgctaccg aaccgcgggc ggggtcgtac gcgtcgcgct
     2641 tgccgagcgc gagcgtgaac gtctcctgca cgacatcttc agcgagcgcg gcgtcacggc
     2701 cgacgcgata aaagacaaac gcgtagagac catcgacgtg ggcatcatag aaacgcccga
     2761 tcgccagccg gtccccatcc tgtgccgcac gtaagtcgcc gcctggtccg gatcggaaca
     2821 ggcgcgcgaa cagcgcggcg atcacgaagc gctccctgag gtcatggtgc ttgcatgtgt
     2881 catcaggggt gaccgaagtg tcgatggtgc ctcaaatagc tgcgcgagca acgattccgc
     2941 gcagatggcg gtcataggga tatgcgcatc ctgcccaccg cgcttgcgat ctcggccgta
     3001 gtacacggcg tcgggatcgt gtgggtcagg tcgcgacctg tgcacaagcc cgagcccacg
     3061 aagctagtta cgatcgagac gatcgaggtc gtgccggctc ccgcgcagcg gggcgagccg
     3121 gtacccactg aggtgacgtt gctcgatgac aacagtgtgg cggcgacggc gccgaacgcg
     3181 tcgtcacgtg gcaacaccaa acccggcaag tccaagcaac atctgtccgc gtccgcgacc
     3241 ggcacgaccg agacggctcc cgcgacgaac gagacgccgc cgccgcggag ctcgctcatg
     3301 acgatgcgcc acccggaccg cccgaagctc gagaacggtc cgtctgccgc gttctgggag
     3361 aagttcgagg cgaacacgaa gccgctgcag ccgaaggaga ttgccggcga gcagcgcaac
     3421 gacgagatcg cgaccgccga aggcaacctg agcaaccagc gctggatcga caacgcgacc
     3481 tccgaagagg tcggtggcga gcgcgcgaag ttgatccaga agcgctacga gcgctcgacg
     3541 gccgagctgc agcctgatgg tcagggcacg aaatcggacc accgcacgtt caaggcgaag
     3601 ttcaacccgg acggcacggt cgcgtcgatc gacgacaagg cgaacctacg ccgcaaaggc
     3661 ctcggcgccg agttcgatgt caccgacgcg atgatgcgcg ccaaaggcat cgatccgtac
     3721 tcgagctaca agctcaaggt gctcgacgac acgcgcgagg agcgcgtcgc gatcggcaag
     3781 cgctatcgca cgcagcagct cgcgcagtcg aagcagctga tgcagaagaa cctcgagcgg
     3841 ctgtgggcga tgacgcacga tctcaccgcg cgcaaggcgg gattgttcga gctgtgggat
     3901 gactgcgccg aaaccggcag tgacgagctc gtcgctggtg gtgcctccgc gcgcgcacat
     3961 gttgtcggtt tcatccgcag caaattgccc gaaggcagcg cggacgcgtt tacgacagcg
     4021 gatctcgagc gcttcaataa gcaacgcaag tcacgagagc aattcgcgcc gtaccagacg
     4081 ttgtaagatc ggcgcatgcg cgctgcacga ctcgtcgttg ttctcgcggt gctgtggtcc
     4141 gttcccgccc acgccgacca tggcaaattc ggcctcggcc tgatcgtcgg tcagccgacc
     4201 ggcctcaccg gtgcctacga cctctccgag aaatcacaga tcgatgccgc gctcggcctc
     4261 gcgctgttcg acgaccgcga tttctacatc cacgtcgagt acctctatta cccgttcacg
     4321 ctcgtccacg gtggcgatct cgatctgtcc gcgtacctcg gcatcggcgg ctggatcgtc
     4381 acggtccacg accccgtgat cggtgcgcgt gcgccgttcg ggctctcgct cgacttcaac
     4441 acggcgccga tccagatctt cctcgaggcc tcggtgctcg tctcgatcgt tcccgacgct
     4501 catggcgccg ttcgtggcgc ggccggcttc cgttactact tctgagcctc gtgctaagca
     4561 gggcccatgg gttttgacgg gccgctgatc gccgggcgct atcgcctcgg cccgcggctc
     4621 ggtcatggct cgcagggcga aatctttctc gcacgcgacg agaaagcgaa gggcaccgac
     4681 gacaaggatg tcgtcgtcaa gcggatgacg ccgcgcggta cctggaagtc gttcgagctg
     4741 ttcgaacgcg agacgaaagt cctcgggcag ctccgccatc cgggcgtgcc gcgctacctc
     4801 acgaccgtcg aagagccgcc cggcacgttc aacctcgtca tgcagcgcat gcccggcgag
     4861 aacctgcgcg atttcgcgaa gcgccgccgg ctctccgaga ccgagctgcg cgacgtgctg
     4921 atccggtgcc tcgagattct cgactatctg cacacgcgca cgccgcccgt cgttcatcgc
     4981 gacatcaagc cgtcgaacat catgcgcgca ccgatcgatg gccgctgcgc gctcgtcgat
     5041 ttcggcggcg tgctcgatgc ggcgcgcgaa aaaggtggct cgacgatcgt cggaacgttc
     5101 ggctacatgg cgcccgagca gcttcacggt caggcggtgc ccgcgaccga catctacgcg
     5161 ctcggtgcga cgatggtcgc actcgcaggc ggcgtcgagc ccgaggacgt gccgcgcaag
     5221 ggcctcaaga tggatctcga caagcacctg ccgaatctcg atccgacgtt ccgcgaggcg
     5281 ttgatcgcga tgaccgatcc tgatccggac caccgtccgc aacgcgcgcg cgaggtcgtc
     5341 gcgctgctcg cgaaggcccg cccgccgaag cgccgcgacg agaaggcgct cgtcgcccag
     5401 ggcacgaaag aagtcgcgcc gcgccgcatg ttctccgacg tgcaggagcc gctcggcacg
     5461 ctgctccgca tcggtgtgct cggcttcggc ttcggcggct gggtcggcat ggcgtcgatc
     5521 cgcgtgtcgc tgatgctcac gatctacatc ctcgcggcac tcggatttcc gaagcgccgg
     5581 cgcgtgctcg aggtcggcca cgagctcgat gcgatgctcg ccgaaggcca aggtgggttc
     5641 acggacatga tgaaaggcgc gatggcaagg cgatgacgac gaagctgatc acgatcccgt
     5701 tctcccacta ctgcgagaag gcgcgctggg cgctcgatcg ctgcggcatt gcgtacgaag
     5761 agcgcggcca cttgccgctg ttccactacc tcgactcgta tcgcgccgca cgccagcgca
     5821 cggtgccgat cctcatcgac ggcgacacgc tggtgaagga ctcgacggac atcatcgcgt
     5881 gggccgatgc gaagcgcccc ggcacactga ttccggtcgc cggtgctgag aacgtgctcg
     5941 cgatcgaaga cgatctcgat aatcacttcg gtccgcacac acggcgctgg ggctactggt
     6001 acctgctgcc aaatcgcgcc gcgctcgagt acatcaaggt cggcgtgccg cgctgggagg
     6061 cgtcgttgct cgggatcagc cggccgctcg cggctgcgtt ccttcgtcgc ggcctcaaca
     6121 tcaacgccga gagcgtcgag cgctcgcgca agaagatcga tgcgacgttc gatcgcatcg
     6181 agcagttgct cggcgatggc cgccgctacc ttgcaggcga tcgcttcacg gtcgccgacc
     6241 tcacgtttgc gtcgctcgct gcgccggttc tggtcccgcc gaaccatccg ttccagacgt
     6301 tcgatctcga cctgttcccg gccgaggcac aggaccaagt cgcggcctgg cgcgcacggc
     6361 ccgccggcaa gctcgcattg cgcctctacg ccgacgaccg cgccgtgctt cccaaggcgg
     6421 cgtagaaagt tcgtggggtc agaagacccg tgcacacggc tgtctgcacc ggattccgcg
     6481 gcccctgacg actgcagttg ctagcagcag attcgcgccg ttagcgagcg tatgccgcac
     6541 tgctcccggg catgtcggct gcaattcccg tcggcatgat gaggctcctc gccgccgcgc
     6601 ttctcgtctc cgtgattggt tgctcgtcgt ccgaagaagg caaagccgac acgcccccca
     6661 cgctcgaggt aacgagcccg gcgcgcggca ccttcgcaga cgccgacacc gtcacggtgt
     6721 ccggccgtgc gaccgacaag gaaggccccg tcaaagtcac cgtcggcggc atggcagtca
     6781 cgcctgccgc ggacggcacg ttcaccgcga cggtgccggt caccccgggc gtcgatctca
     6841 tcgagacgca cgcgatcgac agcgccggga ccgacgtccg cgacgtgcgc gccgtgctcg
     6901 ccggcacgct tgggccgacg gatggcaccg cgaagggcca cgtcggtgca cgcgccggcg
     6961 tcaccgcgct gcgcgccgtt ggcaatgcag tcgccgcgac cgcgaagacg atcgacttca
     7021 ccgccgccgc gcaggcgctc aacccggtct acaacaacac cggctgcctc ggcgcgaaga
     7081 tcgacatcac gagcatctcg ctcacgaatg tcggcgtgaa cctcgcgccg cagaccaacg
     7141 tgctcgcgac cgacgtcacg atcgacaacc tcgacgtccg gctgcacgcc gatttcaagg
     7201 tcgcttgcat cggcggcagc acgacgatca cgatccgctc gtcgaaggct cacgtccatg
     7261 gcgacctcgc ggcggccatc acggccggca agatcacgac gtcgctgccg gccactgccg
     7321 tcacactcga caacttctcg atcgacgtcg gcggcgtgcc gggcgcgatc gagagcctgc
     7381 tgaaagacca ggcgcgctcg ggcgtcgaga aggcgctcac gagcgcgatc aagagcaagg
     7441 tgccgccgat cgccgacaag gcactcgccg gtctgatcgc gaagccggtc accgcctcgc
     7501 tgctcggtca cgacatgacg atcggcatca cgccgacgaa ggtctcgatc acgccgagcg
     7561 agctgttcgt cacggtcgac accacgctgc gcgtcgccgg tggcgaaggc gggacgttcc
     7621 tcaccaccga catggcgctc acgtccgcga cgatgggcag ctcgcaaggg cttggcatcg
     7681 cgctcgacga cgacatcgtc aaccagctat tcgccggcct ctgggccgcc gacgcgttcg
     7741 actacacggt ctcgatcgac accatcccgt cgctcggcgc ggtgctcgac gacgacgcgc
     7801 gctcggtcga gctgaagctg tcgctgccgc cgacggcatc gaccgacagc ggtgagctcg
     7861 agctcgcgat cggtgacctc atgatcagcg tgaaggacga tgcgggcgcc gagctgcagt
     7921 cgatcgcggt ctcgatcacg acgacggtca gcgccgagcc gagccagacc ggcaaggtgt
     7981 tgctcacggt tggcgcaccg accgtctacg cgcaggtgct ggcgcagacc gccgcggtcg
     8041 acgatccgct caccgaccag caggtcgagg cgctcatcag cggcgtgtgg ggcctcatcg
     8101 gcgtgaaggc cgacgatgcg ctctcgaagc tgccgatgcc gacgattgcg ggcatctcgc
     8161 ttggcgcgcc gacggtcacg acgagcacgg gctacgtgct tgccgacatg agcgtgaact
     8221 aaccgccgag cccagaatca accgccaaga cgccgcagac gccaagatca gaagcgcctt
     8281 ggatcgcgcc ctgctcgcgc gcagctccgc gcagaccacg ctcgtgtgga cgtccgcgat
     8341 agtgacgcct tgtcgccagc acgttgatca gaccgcacga cagcacggtt ctgaagagga
     8401 ggcttctcgg tgcttggcgt ctgcggcgac ttggcggtta acccgcgcag cgacgcgtca
     8461 gtcgctcggt ttgacgaaca gaaccttgcc gttgtcatag cgatcttcga cggcgccgtg
     8521 cttttcacgc agcccgcgca gatcggcctc gtcctcgccg agtgcgacac cgaccgcgat
     8581 ggcgttgtcg gtcagcgtgg tctcgatcca gtagtcctgc aggacgctct cttcgatctt
     8641 cgcgccgcgc gcgacgagca gcttcacgat ctccgcgtgg tcaggaaggt gctcccacgc
     8701 gatcgcggcg caatgcagcg gccgcgggtg cggacccgcc gccgagatcc attcccacgc
     8761 atcgtcatcg gccgcgttgc cgttcggatc cgcgcccatg tcgagcagcg ccttcaccgc
     8821 gtcgagcttg ccggcgtaac acgcttcgtg aaggctccac gaggccccgt cggcgtccgt
     8881 ccagcgcata cctaacggta ccatcaactg cgccacgctc gcgcaagtga cagcgtctgc
     8941 gcatggtcag gcaccgcctc gacgaacgag cgccggagct ccgcgtcgcc gatgagcgcg
     9001 gcgtcggcaa gaacgcgctg ctcggcatcg cagatcacgg cgatcgcctc ctccacgcgt
     9061 cggttcgcgt gcagaaacgg gagctgcgtg agcgcagcta caacgcttac gcggcgcgga
     9121 gtgtgagaac agcgacctcg gcgtcggttc cctcaccatg tcggcgcgtg actcccgaga
     9181 agccaacgcc aggtgtcacg tatagcgttg tcttgccgaa cgcgaagtga ccgcgccgat
     9241 agtgcagacc gatcttcttc atcagccgat cagtgatgcc cttgatgtag atctggccgc
     9301 cgtgcgtgtg accgctcaac acgagatcgg cgccgcgctc ggcgagccga ggtgctgcat
     9361 cgggcgcgtg gcacaacacg accttcgtgt gcttctcgtg cgtgcccttg aacgccgcat
     9421 ctcgatcgtc gtggcgcgtg accggatcgt ccacaccgac gagcgcgagc ggtgcaccgt
     9481 tgatctgcgc caccgtgtgc tgattcttca gcacctcgta gccgttcttc tcgagcgcag
     9541 acgcgacgcc gcgtccccac gtgaagtagt cgtggttgcc gagcaccgcg agcacgcgcg
     9601 aggctgtcag gccgctcagc tgatcgcccg ccatctccgc ctcgtgccgg cgccagcaga
     9661 cgtagtcgcc ggtcaatacg atgatgtctg gcttcgcgcc gttcgccgcc tcgaccgccg
     9721 cgcgcacatg cgaggcgggg gtcatgttgc cgacatgaat gtcggatagc tgggcgatcc
     9781 gcacgccatc gtgccgagga tcgaggtctt tcacgcggac ctcgtgctcg gtaacccgcg
     9841 gcggcggagg aggcattggc accactgtgg cgcctggccg ggagcttggc aatgtcggcg
     9901 gaatagccgg cgcggcgcgg ggtgtttcac ccttccagaa ccgattgcag gagtctgcca
     9961 aggatgacag cattagccca catggccgcg gtgcgccctc ctggtatcgc tgacgcgcgc
    10021 gtgccgtgcc cgctctgcgg gggtctcatt caccccgtcg caggacgctg caagcactgc
    10081 aaagaagatc tgagctcgtt ccgcgaaggt cgtccgcaag ctgcagccgc gctgcccgcc
    10141 ttgaacggca agcctgccca gacaggcggg atggcaaacg gcagcaacgg gcacgcggcg
    10201 gcaaccgcga ttccagccgc gatcccgacg cacgaagcgt cgcagccgat cctgccgccg
    10261 cgcacgaccg cgcgctcgat gcaggcgcag cggccgagcc acagcatctg gcgcagctgg
    10321 ccgatgctcg tgatcgtgct cgcggtgatc gcgatcgtcg ctgcgaccgt gatcatgatc
    10381 cttccgccgg gcagcaagaa gcacgacggc aagatgtcgg cgccgccggg tcccgcgttc
    10441 gagcggatgg agaccaaccc gctgcccgac aaatcgagcc agctcgatcc atgggacaac
    10501 ggcgcgcaag gcgcgacgcc gcgtgctccc gatcggcaac cgcaaccgca ggcgccgacg
    10561 ccggtcgatc ccgacgatga tctgtggaac ggcagcggcg gcatcgccgg cggtggctcg
    10621 ttcggcatga ccggcaacgc gttcatgatt cacgtgctcg atcgcgcctg caagaagctg
    10681 aagacgtgcc cggatctcga tcagtcgtcg ctgtcgacga tctgcgagag cgtcgcgatg
    10741 atgccgaagc cgccggcgcc gacctgtgcc gccgctcaga agtgcttcga cgcgatcgat
    10801 cgactgagct gctctcaagc atccgcacag gacatgagcc cggtctcgat attcggtttg
    10861 ttctcggact gcagagacgc cgacaagtgc tgataggctg cgccggtgac gtatctccgg
    10921 cccgaattcg agatcgttcg tgaccctgcc gcgatgcgcg cacgcgccga ggatcttcgt
    10981 cgcgatggcc gccgcattgc cgtcgtgccg acgatgggcg cgctacacga aggtcacctg
    11041 acgctgctcc gcgccgcccg cgcgaagtgc gacatcctga tcctcacgat cttcgtcaac
    11101 ccgacgcagt tcggccccaa agaggatctc gcaaagtatc cgcgtgacga agccggcgac
    11161 ctcgcgaagg cgcgcgcgtg cggcatcgac ctcgcgttct gtcccgacgt caccgcaatg
    11221 tatccaaccg gcgcgcagac cttcatcgag gtccgcgagt tgcagaagcc gatgtgcggc
    11281 gcgagccggc ccggtcactt cgccggcgtt gcgaccgtcg tcgcgaagct gttcaacatc
    11341 acgaagccgc acctcgcggt gttcggcgag aaagacttcc agcagctcgc gatcattcgg
    11401 cgcatggtgc gcgacctcga cttcggcatc gagatcgtcg gcgtgccgat cgtgcgcgaa
    11461 gcagatggcc tcgcgctgtc atcgcgcaac gcgtatctgt ccgccgacca acgccgcgcc
    11521 gcgctctcgc tcaacaaggg actcgccgcc gccgaggccg cattcaaggc cggcgagcgc
    11581 aacgtcggca cgctgctcgc tgccgcgcgc gcaccgatcg acgccgagtc gaccatgcgc
    11641 atcgattatc tcgagctgcg cgacgccgac gagctcacgc cgatcgacac cgtcgcgcgc
    11701 cgtagcgtgc tcgcggttgc tgcgttcgcg ggcacgacgc ggcttatcga caatcgagtg
    11761 ctcgcgccgt aaggcgtcgc cggctttgct accgctgccg gcgtttgcca ggccgacacc
    11821 aggcctacat cggcgtgtgc atcggtgtgc ctcgctattg gcgtcgatcg ataacgcccg
    11881 gtcacgttgc cgatgtttgc acggtcgaac caggcaaccg gtcccgtggc atcgccgttg
    11941 ctgatgtctc gtcggcggag ggcagcatga agaacagcat ccttggtcta gttttgcttg
    12001 gactcacttc gacgactgcg tttgcacaag agaatgcggc gccggccaca ccacctgcgc
    12061 cgacgaccgc ggcggcttca ctcgaaacga agccggcgca gaacgcgctg tacgcggagc
    12121 tcggcggtaa cagcggctgg tacagcatca actacgagcg gttcattcgt cccgacgccg
    12181 cgattcgcgt cggcctcagc tacatgagcg tgaccgcgac ggcaggcagc ggcgagaaca
    12241 gcgcgtcggc gaatgcgacg tgggcgacgc taccggtcat gttcgagtac ctcggcatcc
    12301 atgcgggcag ccatgcgctc gagctcggcg gcggcgtcaa cttcatgtac ttctcgggct
    12361 ccgcggcgac gttcgacgcg accgcgatga gcacgggcat cgtgcccgtc ggcacggcga
    12421 cgatcggcta tcgctactcg aatcccgacg gcggcttcat cttccgcgcg ggctacacgc
    12481 cgctgatctt cgtcagcacc gagcagaagg acattttcca ctggggtggc atgtcgttcg
    12541 gctatcgctt ctgagtccgc gacaaccggt tgagcagcga cgacacccag ccctgcttgc
    12601 ccgacggttc ctcgccacca tccgatgacg ccgctggttc ggcggccacc gcatcgagtg
    12661 ttggcaggac ctcgatttcg ggctcgtcgt cggcaggtgg cgctggtttc gtcagcggca
    12721 ccggctgcgc gttgccgatg tt
//
LOCUS       DASUFX010000808         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_686839,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000808 DASUFX010000000
VERSION     DASUFX010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686839"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFV99_32755"
     CDS             <1..949
                     /locus_tag="VFV99_32755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5064206.1"
                     /translation="RRRLVVSIVVADNVSKWYGHVLGVSDVSWTLRGGIVGLLGPNGA
                     GKSTLIKMMGGLLTPSRGSLTVFGASPFEDIAVRRRIGYAPEHEKTWDELTALELVTA
                     MAKLAGVPSGDAKRKADAALAQMGMDYAKHRRVKGFSKGMRQRTKLATAIAHDPDFLL
                     LDEPLTGVDPKARIEIVEQIKKLGEAGKTIVISSHVLYEIEALTSDIVVIYRGQILAE
                     GNVYEIRKLIDRHPHRIRVECDKPRAIASALAGEEHVARIIFERGAVLVETRDPDRCY
                     EQIGDTVLAANVAVSTLTSPDNNLGAVFEYLTGDGGRHE"
     gene            954..1625
                     /locus_tag="VFV99_32760"
     CDS             954..1625
                     /locus_tag="VFV99_32760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5064207.1"
                     /translation="MPSTASALATLAGVTLRRLSRSRALWVLLAISLLPAFIASFLHG
                     HTTIFDLVATTQLIVTALLASVFVASAIGEEIEDRTTTYLWSRPIARWTIVAGKVIAL
                     APICGLLAAGGWAVAMKWTNSPLELRTTLAFASGGIALSFVAAGIALLVPKHGMALSI
                     IYLLLVDLVIGGIPASLQSISITHQVWLLARTDPGIANPAITMVVIAVLWLGVGLLRL
                     RRLES"
     gene            complement(1622..>2100)
                     /locus_tag="VFV99_32765"
     CDS             complement(1622..>2100)
                     /locus_tag="VFV99_32765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5064208.1"
                     /translation="AFATWGAEEQGMIGSTFFAANPPDALPTGKIVQYINLDMVGSHK
                     SKGFVAAMGTFAKQPARKLLDKQVKAFPKLHVGLGGRAARSDHSPFCKLGIPYVFFWT
                     PDAKCYHEGCDTAEKIDLPRMADIAALAGDLAWSLANGDTDLAASRTKLGCGQKDD"
BASE COUNT          333 a          687 c          702 g          378 t
ORIGIN      
        1 gcgtcggagg ctcgtcgtga gcatcgtcgt cgccgataac gtcagcaagt ggtacggcca
       61 cgtgctcggc gtctccgacg tgagctggac gctacgcggc ggcatcgtcg gtctgctcgg
      121 cccaaacggt gccggcaaat cgacgctgat caagatgatg ggcggcctgc tgacgccgtc
      181 gcgcggctcg ctgacggtgt tcggcgcaag cccgttcgag gacatcgcgg tgaggcgccg
      241 gattggctac gcccccgagc acgagaagac atgggacgag ctgaccgcgc tcgagctcgt
      301 caccgcgatg gcgaagcttg ccggtgtgcc gagcggcgac gcgaaacgga aggccgatgc
      361 cgcgctcgcg cagatgggca tggactacgc gaagcaccgc cgcgtgaagg ggttctcgaa
      421 gggcatgcgc cagcgcacga aactggcgac cgcgatcgcg cacgaccccg acttcctgct
      481 gctcgacgag ccactgactg gcgtcgatcc gaaggcgcgc atcgagatcg tcgagcaaat
      541 caagaagctc ggcgaggccg gcaagacgat cgtgatctcg agccacgtgc tctacgagat
      601 cgaggcgctg acgagtgaca tcgtcgtgat ctaccgcggc cagattctcg ccgagggcaa
      661 tgtctacgag atccgtaagc tgatcgatcg ccacccgcac cgcatccgcg tcgagtgcga
      721 caagccgcgc gcgatcgcga gcgcgctcgc cggcgaggag cacgtcgcgc gcatcatctt
      781 cgagcgcggc gcggtgctcg tcgagactcg cgatccggat cgctgttacg agcagatcgg
      841 cgacaccgtg ctcgcagcga acgttgcggt cagcacgttg acgtcacccg acaacaacct
      901 cggcgcggtg ttcgagtacc tcaccggcga cggagggcgg catgagtaat accgtgccca
      961 gtaccgcctc ggccctcgcg acgcttgccg gcgtaacgtt gcggcgcctc tcgcgttcgc
     1021 gcgcgctctg ggtactgctt gcgatttcgc tgctgccggc gttcatcgcg tcgttcctgc
     1081 atggccacac gacgatcttc gacctggtgg caacgaccca gttgatcgtg accgcgctgc
     1141 tcgcgtcggt gttcgtcgcg tcggcgatcg gcgaagagat cgaggaccgc acgacgacgt
     1201 acctgtggtc gcggccgatc gcgcgctgga cgatcgtcgc cggcaaggtc attgccctcg
     1261 caccgatctg cggcctgctt gccgcgggtg gctgggcggt cgcgatgaag tggacaaact
     1321 cgcctctcga gctgcggacg acgctcgcgt tcgcgtcggg tggcatcgca ctgtcgttcg
     1381 tcgcggcggg catcgcgctg ctcgtgccga agcacggcat ggcgctctcg atcatctatc
     1441 tgttgctcgt cgatctcgtg atcggcggca ttccggcgtc gctgcagtcg atcagcatca
     1501 cgcatcaggt gtggttgctc gcgcgcacgg atccggggat cgccaatcct gcgatcacga
     1561 tggtcgtcat cgccgtgctg tggcttggcg tcggcctctt gcgcctacgc cggctcgagt
     1621 cttagtcgtc cttctggccg cagccgagct tcgtgcgcga cgcggcgaga tcggtgtcgc
     1681 cgttcgccag cgaccacgcg aggtcgccgg cgagcgccgc gatgtctgcc atccgcggca
     1741 gatcgatctt ctccgccgtg tcgcaaccct cgtggtagca cttcgcgtcc ggcgtccaga
     1801 agaacacgta cggaatgccg agcttgcaga atggtgagtg atcgctgcgt gccgcgcgcc
     1861 cgccgagtcc gacgtgcagc ttcggaaacg ccttgacctg cttgtcgagc agcttgcgtg
     1921 ctggctgctt cgcgaacgtg cccatcgccg cgacgaagcc cttagacttg tggctgccca
     1981 ccatgtcgag gttgatgtac tggacgatct tgccggtcgg cagtgcgtcg ggaggattcg
     2041 cggcgaagaa cgtcgagccg atcatgccct gctcttccgc accccatgtc gcgaacgcga
//
LOCUS       DASUFX010000809         6320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_686842,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000809 DASUFX010000000
VERSION     DASUFX010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686842"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFV99_32770"
     CDS             <1..852
                     /locus_tag="VFV99_32770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064209.1"
                     /translation="VQHCGATVGAVPEAMTLNIAAQLCDALDHAHTKKDAKGRSLGII
                     HRDISPANIILSTNGVVKLIDWGLAKAQLEKEDTGETHIKGKYGYVAPEYLEGKLSAR
                     ADLWAVGIIIYELLTSRRLFDGADDFQTVQRVKNLPIPRPSIANPRVTPELDAIVMKA
                     LERDPTYRWKTAAEMRDALREVIAQPGMHTDNDHIAQWVQWVFTQKPGTAASGVSHLA
                     SIAQPQRFPSMPPHAPEDTTVPSGPPIAMESDGAIGAITSNVRSYPWFWIVLAFIVAL
                     LIVWRVS"
     gene            complement(849..3062)
                     /locus_tag="VFV99_32775"
     CDS             complement(849..3062)
                     /locus_tag="VFV99_32775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5064210.1"
                     /translation="MSFDLGARDRPAPAVPSLRLFADTLFTEDGAEVHTGMLGLACDR
                     RIEDQARRILEGLGAIDLEYLDDCAVTPGAGIDYVVSLDGDIHSLCAFTAYAVPQLRA
                     LGWRVDVDADYPWQVVANDAPLYAAALPDRERPDWFGLELGVEVDGQRVDLLPALLDM
                     LDTAGDLAALTRSARRCIAVRVDDKRWLPVPPARLKLLAKVLVELYREGGKGKVRAPA
                     VRAPLVVELCATLHDGTRPLRWVGDPKVREQAFALALGPRPAAVAATPTNLRAELRPY
                     QRDGVAWLQHLRAHDAGGVLADDMGLGKTLQTIAHVLTEKEQGRLDRPAMIVTLTSLV
                     GNWRRELERFAPTLTVAVHHGADRHERREELTNRDVIITTYPLVARDRDDLADVPLHL
                     LVLDEAHSIKNSGAQASEAVRRLDARHRVCLTGTPIENHLGELWALFDFLNPGLLGSK
                     EEFALQFRQPIEERGDKVRLEALRDRVRPFILRRTKDAVAPELPHKTQLVRAVELTGA
                     QRELYESIRVAAHGQVRQHIRQRGFAASQIAILDALLKLRQVCCDPRLVSVDAARDVT
                     GSAKLDVLLELLGQQLADGRRILVFSQFARMLGLISESLLARGVRHVALTGHTPDRQK
                     PIDAFQQGKVDVFLISLKAGGAGLNLVGADTVIHYDPWWNPAAQAQATDRAHRIGQTK
                     PVFVHDLIVAGSVEERMLSLQRHKRLLATAILDKGNTAGPTLSERDLDDLLAPLA"
     gene            3243..3641
                     /locus_tag="VFV99_32780"
     CDS             3243..3641
                     /locus_tag="VFV99_32780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5064211.1"
                     /translation="MKPTELTVADLMSTNVITINASDPIKEGRADMDLGVIRHLPVVD
                     DRGRLVGVVSDRDLIAPKRAHKIADVMTRDVMTARPETPAFEAASMMLDHKIGSVLVV
                     NDENKLVGVVTQTDYLEVARRALLGLSLER"
     gene            complement(3645..5600)
                     /locus_tag="VFV99_32785"
     CDS             complement(3645..5600)
                     /locus_tag="VFV99_32785"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5064212.1"
                     /translation="MTPTREDLELYVTGNYDGDVAALERAIADDPALAAIVAEEARLD
                     MLLRDAAASATFCVACNALVRNERCDDCGAAVKPGGYTVERVLVSNAHGRMYVAYDAD
                     GKRVALKELAFVHAPSASAIAAFEREAKFLRALEHPAIPRFCASFEEGKGVHTRYYLA
                     QELVEGKALDQLDEHWYTEAEIIDIAKQVLAILVYLQSLSPMVIHRDIKPANLLRRTD
                     GSIALVDFGAAYVQGATAGFTTIGTFGYMPIEQLAGIVDATTDCYALGATLLNLLTRE
                     EPWRLAQTKTNVNASAPLRAFLDKLIATEPRERFPSAKDALAALDKRDALVVAKPLPR
                     ARARKWPIVGAAVAAVAIGGGIAAFSLSSSEPQVMVKPTVVADGFGHVRIRLVGGAGV
                     AKGLIDGKVMVVKNNQLFELSAGSHRISIIASADTRCEQDIVVKDGETMTLECNLPDV
                     EVELAPLRDMPKLKADKIISWEFKDVPLQDVLMLAGATCNVNIVVPDYIRGRVTVDLD
                     KVQCDQAIESILEAQGLWYSYSPVGNLMRVAPRKDLDEEARAPARANAATLPPGKTID
                     INIKDADLHDALKVITLGSGVNLVIPDYIRGKLTVRFKNVPWEQAFEAALASQGLWYR
                     YRENGHIITVAPRKDIEAEDAAHGKRP"
     gene            complement(5597..6103)
                     /locus_tag="VFV99_32790"
     CDS             complement(5597..6103)
                     /locus_tag="VFV99_32790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5064213.1"
                     /translation="MPPPAHVWDDAIRRHDRRVYLSVLALGMAPDRAREIVQAAWTRL
                     IEQHAAGALTELDFPGLAIRQARFLALNELQRRKVEMRVLAAVPEAAPEPDVDRVVAS
                     RQQMERVLDALATCSPTAKQVFRLVYATPGGNCASAAQAVGLSLQRVRQILCETRNHI
                     RRALEGTP"
BASE COUNT         1068 a         2088 c         2122 g         1042 t
ORIGIN      
        1 gtccagcact gcggcgcgac ggtcggtgcg gtgcccgagg cgatgacgct caacatcgcc
       61 gcgcagctgt gcgacgcgct cgatcacgcg cacacgaaga aggacgcgaa ggggcgctcg
      121 ctcggcatca tccatcgcga catctcgccc gcaaacatca tcctgtccac gaacggtgtc
      181 gtgaagctca tcgactgggg cctggcgaag gcgcagctcg aaaaagagga caccggcgag
      241 acgcacatca agggcaagta cggctacgtc gcgcccgagt acctcgaagg caagctatcc
      301 gcgcgcgccg atctgtgggc ggttggcatc atcatttacg agctgctgac gagccgccgg
      361 ctgttcgacg gcgccgacga cttccagacc gtgcagcgcg tgaagaacct gccgattccc
      421 cggccgtcga tcgcgaatcc gcgcgtgacg ccggagctcg acgcgatcgt gatgaaggcg
      481 ctcgagcgcg atccgaccta tcgctggaag accgctgccg agatgcgcga cgcgctccgc
      541 gaagtgatcg cacagccggg catgcacacc gacaacgacc acatcgcaca gtgggtgcaa
      601 tgggtgttca cccaaaaacc gggcaccgcg gcgagcggcg tctcgcacct ggcgtcgatc
      661 gcgcagccgc aacggtttcc gtcgatgccg ccgcatgcgc ccgaagacac cacggtgccg
      721 tcagggccgc cgatcgcgat ggagagcgac ggcgcgatcg gcgcgatcac gtcgaacgtg
      781 cgcagctatc cgtggttctg gatcgtgctc gcatttatcg tcgcgctgct gatcgtctgg
      841 cgcgtcagct aagcgagcgg cgcgagcaga tcgtcgaggt cgcgttcgct gagcgtcggg
      901 ccggcggtgt tgcccttgtc caggatcgcg gtcgcgagca ggcgcttgtg gcgttgcagc
      961 gacagcatgc gttcttcgac ggagccggcg acgatcaagt cgtgaacgaa caccggcttc
     1021 gtctggccga tgcgatgtgc gcggtcggtt gcctgcgcct gcgcggccgg attccaccac
     1081 gggtcgtagt ggatcacggt gtcggcaccg acgaggttca ggcccgcgcc gccggctttg
     1141 agcgagatga ggaatacatc gaccttgcct tgctggaacg cgtcgatcgg cttttgacga
     1201 tccggcgtgt gcccggtcaa cgcgacgtgg cggacaccgc gcgcgaggag tgattccgag
     1261 atcaggccga gcatgcgagc gaactgcgag aacacgagga tgcggcggcc gtcggcgagc
     1321 tgctgtccga ggagctcgag cagcacgtcg agcttcgcgc tgccggtgac atcgcgtgcg
     1381 gcatcgacgg agaccaggcg cggatcgcag cacacctggc gaagcttcag caatgcatcg
     1441 aggatcgcga tctgcgacgc tgcgaacccg cgctgacgga tgtgctggcg gacctggccg
     1501 tgcgcggcga cgcggatgct ctcgtagagc tcgcgctgcg caccggtgag ctcgaccgcg
     1561 cgcacgagct gcgtcttgtg tggcagctcc ggtgcgacag catctttcgt gcggcggagg
     1621 atgaacggtc gcacgcggtc gcgcagcgcc tcgagccgca ccttgtcgcc gcgttcctcg
     1681 atgggttggc gaaactgcag cgcgaactct tctttgctgc cgagcagccc gggattcaag
     1741 aagtcgaaca acgcccacag ctcaccgagg tggttctcga tcggcgtacc ggtgaggcag
     1801 acgcgatggc gtgcgtcgag ccggcgcacc gcttcgctcg cctgagcacc gctgttcttg
     1861 atcgagtgcg cctcgtcgag cacgagcagg tggagtggca cgtcggcgag atcgtcgcgg
     1921 tcgcgcgcga cgagcgggta ggtcgtgatg atgacgtcgc ggttcgtcag ctcctcgcga
     1981 cgttcgtggc gatcggcgcc gtgatgcacc gcgaccgtta gcgtcggcgc gaaccgctcg
     2041 agctcgcggc gccagttacc gacgaggctc gtcagcgtga cgatcatcgc cggccgatcg
     2101 aggcgccctt gttctttctc ggtcagcacg tgcgcgatcg tctgcagcgt cttgccgaga
     2161 cccatgtcgt cggcgagcac gccaccggca tcatgcgcgc gcaggtgctg gagccaggca
     2221 acaccgtcgc gctgatacgg acgtagctcg gcgcgcaggt tcgtcggtgt cgccgcgaca
     2281 gctgcgggac gtgggccgag cgcgagcgcg aatgcctgct cgcgaacctt cggatctccg
     2341 acccagcgca acggtcgagt gccgtcgtgc agcgtcgcac acagctcgac gaccagcggg
     2401 gcgcgtaccg ccggcgcacg caccttcccc ttgccgccct cgcggtacag ctcgacgagg
     2461 actttcgcga gcagcttgag ccgcgcgggc ggcaccggca gccagcgttt gtcgtcgacg
     2521 cgtaccgcga tgcagcgccg cgccgaccgc gtcagcgccg cgaggtcgcc ggccgtgtcg
     2581 agcatgtcga gcagcgcggg caacaggtcg acgcgctggc catcgacctc gacgccgagc
     2641 tcgagcccga accaatcggg tcgctcgcgg tcggggaggg cggcggcgta gagtggcgcg
     2701 tcgttcgcga cgacttgcca cggatagtcg gcatcgacgt cgacgcgcca gccgagggcc
     2761 cgcagctgcg ggacggcgta cgcggtgaat gcgcagagcg agtggatatc gccatcgagg
     2821 gaaactacgt agtcgatgcc ggcgccgggc gtgaccgcgc agtcatcgag gtactcgaga
     2881 tcgatcgcgc cgaggccttc gagaatgcgg cgtgcttgat cttcgatccg gcggtcgcag
     2941 gcgagcccga gcatgccggt gtgaacctcc gcgccgtcct cggtgaacag cgtgtcagca
     3001 aacaatcgca gcgacggaac tgcaggcgcg ggccgatcgc gcgcccctag gtcgaacgac
     3061 atcggagcat ccacattgag ggcgccgatc tgatccgaca agttttcgcc gcgcagcgag
     3121 agatctcctc acgtcgaaaa tgcgcaccgg ctgcgcttgc aaacgggagt tgccgcccaa
     3181 acgccgcgat tgcgcgcaaa ccaccctcac gcgatggcac gttgctcgct actccagagg
     3241 ccatgaagcc caccgagctg accgtcgcag acctgatgtc caccaatgtc atcacgatca
     3301 atgcgagcga tccgatcaag gaaggccggg cggacatgga cctcggcgtg atccggcacc
     3361 taccggtggt cgatgaccgc ggccgcctcg tcggcgtggt ctccgatcgc gacctgatcg
     3421 cgccgaagcg tgcccacaag atcgcggatg tcatgacgcg cgacgtgatg acggcgcggc
     3481 cggagactcc cgcgttcgaa gcggcatcga tgatgctcga ccacaagatc ggctcggtac
     3541 tcgtcgtcaa cgacgagaac aagctggtcg gcgtcgtgac ccagaccgat tacctcgagg
     3601 tcgcgcgccg cgcgctgctc gggctgtcgc tcgaacgctg atcgctacgg tcgcttgccg
     3661 tgcgcggcgt cctcggcttc gatgtccttg cgcggtgcga ccgtgatgat gtggccgttc
     3721 tcgcggtacc ggtaccacag gccctgcgac gccagcgcgg cctcgaacgc ctgctcccac
     3781 ggcacgttct tgaaccgtac ggtcagtttg ccgcggatgt agtccgggat caccaggttc
     3841 acacccgagc ccagcgtgat gacctttagc gcatcgtgca ggtctgcgtc cttgatgttg
     3901 atgtcgatgg tcttgcccgg cggcaacgtc gctgcattcg cccgcgccgg cgcgcgcgct
     3961 tcctcgtcga gatccttgcg cggtgcgacc cgcatcaagt tcccgacggg gctgtagctg
     4021 taccagagcc cttgcgcctc gaggatggac tcgatcgcct ggtcgcattg gaccttgtcg
     4081 agatcgacgg tgaccctgcc gcggatgtag tcggggacga cgatgttcac gttgcacgtc
     4141 gcaccggcga gcatcagtac gtcctgcagc gggacatctt tgaactccca cgagatgatc
     4201 ttgtcggcct tcaacttcgg catgtcgcgt agtggcgcga gctcgacctc gacgtcgggc
     4261 aggttgcact cgagcgtcat cgtctcgccg tccttgacga cgatgtcttg ctcgcagcgc
     4321 gtatccgcgg acgcgatgat cgagatgcgg tgagagccag cggagagctc gaacaactgg
     4381 ttgttcttga cgaccatcac tttgccgtcg atcagaccct tcgcgacacc cgctccgccg
     4441 acgagacgga tccgcacgtg gccgaagcca tcggcgacca ccgtcggttt caccatcact
     4501 tgcggctccg agctactcag actgaacgcc gcaatgccac cgccgattgc cactgctgcg
     4561 accgccgcac cgacgatcgg ccacttgcgt gcccgtgcac gcggcagcgg cttggcgacg
     4621 acgagcgcgt cccgcttgtc gagcgcggcg agtgcatcct tcgcggacgg gaaccgctcg
     4681 cgcggttcgg ttgcgatcag cttgtcgagg aacgcacgca gcggcgccga cgcgttgacg
     4741 ttcgtcttgg tctgcgcgag ccgccacggc tcctcgcgcg tgagcagatt gagcagcgtc
     4801 gcgccgagcg cgtagcaatc ggtcgtcgcg tcgacgatgc cggcgagctg ctcgatcggc
     4861 atgtagccga acgtgccgat cgtcgtgaac ccggcagtcg ctccctgcac gtacgccgcg
     4921 ccaaagtcga cgagcgcgat cgagccgtcc gtgcgcctca gcaagttcgc cggcttgatg
     4981 tcgcgatgga tcaccatcgg cgacaggctc tgcagataga cgaggatcgc gagcacctgc
     5041 ttcgcgatgt cgatgatctc ggcctcggtg taccagtgct cgtcgagctg atcgagagcc
     5101 ttgccctcga cgagctcctg cgcgaggtag tagcgcgtgt gcacgccctt gccctcctcg
     5161 aagctcgcgc agaatcgcgg gatcgcgggg tgctcgagcg cgcggaggaa tttcgcctca
     5221 cgctcgaatg cggcgatcgc cgacgccgat ggtgcatgca cgaacgcgag ctccttgagc
     5281 gcgacgcgct tgccatcggc atcgtacgcg acgtacatgc gcccgtgcgc gttgctgacg
     5341 agcacgcgct cgacggtgta gccgcccggc ttcaccgcag caccgcagtc gtcgcagcgc
     5401 tcgttccgca cgagcgcgtt acacgcgaca cagaacgtcg cggacgcagc ggcatcgcgc
     5461 agcagcatgt cgaggcgagc ctcttcggcg acgatcgcag caagtgcagg atcgtcggca
     5521 atcgcacgct cgagcgcggc gacatcaccg tcatagttgc ccgtcacgta gagctccaag
     5581 tcttcgcggg tcggcgtcat ggcgtaccct ccagcgcacg acgaatgtgg ttgcgcgtct
     5641 cgcacaggat ctggcggacg cgttggagcg acagcccgac ggcttgcgcg gcactcgcgc
     5701 aattacctcc cggcgtcgca tagacgagac gaaacacctg cttcgcggtc ggcgagcagg
     5761 tggcgagcgc atcgagcacg cgctccatct gctggcggct agcgaccacg cgatcgacgt
     5821 cgggttcggg agctgcctcc ggaaccgcgg cgagcacgcg catctcgacc ttgcgccgct
     5881 gcagctcgtt cagcgccagg aacctggctt gccggatcgc caggcccggg aaatcgagct
     5941 cggtcagggc accggctgcg tgctgttcga tcagccgcgt ccaggccgcc tgcacgattt
     6001 cacgggcccg gtccggggcc attccgagcg cgagcaccga tagatagacc cgccggtcat
     6061 gacggcgaat cgcgtcatcc cagacgtgcg cagggggagg cacggtttgc atcgcttcca
     6121 cgtgacaaga acaggcgagc gcgcgaacca tatcgcatga aaaacctcgc agatcgcgca
     6181 gcgtggatta ccggggctgc cagcggcatt ggcctcgctc tcgcacaccg gctagccgcc
     6241 gaaaagatga agctcgtgct cgtcgatgtc gaggcgggcc cgctattgga ggccgaggcc
     6301 gcgctgcgtg gcaaaggggc
//
LOCUS       DASUFX010000810        14585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1242337, whole genome shotgun sequence.
ACCESSION   DASUFX010000810 DASUFX010000000
VERSION     DASUFX010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14585
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242337"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(458..2467)
                     /locus_tag="VFV99_32795"
     CDS             complement(458..2467)
                     /locus_tag="VFV99_32795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064214.1"
                     /translation="MRTWTLVLLTLLAVSCKRRQEPQPREAPKTGSGSAAAAAAHDQI
                     ARPDFNRFAVRLNIPVYWIVDGNNNKVLDADEVAPLLFYGTSPTWTADGKFTADFEAA
                     YNKIVAASKEPPLDTSTEDGKRRALVRQDLDAGRATLVRSDFSTLSADDKTFVKRMLA
                     IGDQIDDLFETTNGSKAVVAQLPADPESRSLFRRNRGAKCEGPVSSKDPACSPLPGAP
                     KVPVDLYPAAIQTKDTFCKDLQARKDGEALMGHFNVVRGEGDALKPVPYTEAYKDKMT
                     AIANALTETANGIKDPNEQPLVAYLKAAAASFGSNDWVPADEAWAKMSVDNSKWYVRV
                     APDETYWEPCAQKAGFHLSFARINQGSKEWQARLVPVQQEMEDAIAKVAGAPYKARKV
                     TFHLPDFIDIIINAGEDRAPLGATIGQSLPNWGPVANEGRGRTVAMVNINTDPDSRAA
                     RKQQAESLLDAASAKLYPESTEPGLLSTILHEATHNLGPAHEYKVNGKDDDVIFTGPI
                     ASMMEELKAQSGALYLLDFLRQKKLISDEMAQQSFVDSIIWAFGHISQGMYTGEHNRK
                     AYGNLAAIQVGYLIEKGALTWDANATAANGTDKGAFTIHADKLVPVANDLMKEVAGIK
                     ARGDKAAAEKLIAKYVDSETVVPHKLISERMLRHPKPSFVYSVGM"
     gene            2507..3478
                     /locus_tag="VFV99_32800"
     CDS             2507..3478
                     /locus_tag="VFV99_32800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064215.1"
                     /translation="MRGCINALVLAVAAVAAVAADAGARSGEPIVFVGADPAFSSALD
                     DALLPAGMDVLPIGILPAPALAEFAARSRELADREHATATVWLLPAQSGATLVAYDRG
                     VDRLLVRELPYRLPLSATQAAEAARMVRTMLRALRVTPDSELAPPPVAPTGPPQPLIA
                     ASLGIGAWFAAPGTDSALAGSLTVAWRPHGLGAALTGMLAPSADVMSVTFEGRISDIV
                     IAAEARKALLLPPAIRVTPAAGIALHLLRLTGSLGGGSELSSRRYDPAARLGVTAGYA
                     LPRGLEVGLSVSADCLLQRQRYQVASEEILVIPRLQVVTGIVVGLRL"
     gene            3521..4117
                     /locus_tag="VFV99_32805"
     CDS             3521..4117
                     /locus_tag="VFV99_32805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5064216.1"
                     /translation="MGLSIVERPTPEDDAELVAACVAGDMAAQRALFRREYPRVNATV
                     YRLIGSARDADDLVQETFIAVFRGLPRFRGDSKLATWIDRIAVRVVFHHIRDRGRAPI
                     PLDVIGDVEGNGGSTDAQAHARDGLRRFYAALAELTPEARTAFALFAIDGRSISEVAQ
                     VTQTSFVAAKCRIWRARREIMKKAKDDPVLAELVKEDR"
     gene            4114..5262
                     /locus_tag="VFV99_32810"
     CDS             4114..5262
                     /locus_tag="VFV99_32810"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5064217.1"
                     /translation="MKRIVVDPPVSEARRERVERQLFQQLAAVRVADRAEAVIPPPKK
                     SRAAMWWGAAACAAAVVATVLFITRDGATGGQLAQPSRVVTPVDSESTFTIGHEAVVV
                     AKRDTSVEWHQGRDGSITLQLARGAVDCQVEPRHGRPPFKVVSGDITVVVVGTRFTVT
                     RTPSPRVDVAHGKVRVEAPGGTWFVEAGESWTPISVTAIEEPDQASDPGLQASGPAPE
                     IEMDPIKVSRAKKAAAPPAPESDSGRSLKREARSPSSPIAPPETRELAKEAYRAAQHL
                     ENTDPKLAAANYRAIALGKDDGWAATALVALAELDLKQNEPQAALDALDEHARRFPRS
                     AMREDAAWFRIEALRSMGKRDEARGAAADYLREFPDGVYVKPAARLVK"
     gene            5356..7050
                     /locus_tag="VFV99_32815"
     CDS             5356..7050
                     /locus_tag="VFV99_32815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064218.1"
                     /translation="MFLLALVGCTETVHLSRDPLEGLVSLELTPFDSTIKITDIAPPH
                     HTLQFLAMGHFEDGTTRDITVLVEWAIDNALLGTFDTRGLFTASHAAAGHGNVTIEAR
                     GLQATTQLTVIIDATVIDPAFPPPGANLFDPSIPFTTDPMRSPTLTYPAETTTFPQNI
                     ASTLFQYERGTGNDAFRIVFDSEVIHLAVETGSDRWQADGDMQRLLAATGITAPIRTE
                     IQARASTMMPAMIYVGNHATLTFTKDAPGGALLFWSAATNGIMRGGVEMQSAGKLYPA
                     STTCVGCHAVSRDGAQLAMGVDNMPTTDLLTVDMGSLSPRIPQSPARPMGWATYSPDG
                     TRLIVANNGVLTQYDSASGMSLGTVPLPAMRYATHPDWSPDGQWIVVAMTSTAPTNMD
                     VKSASIAKIPFNDGAWGTPQIIVSGSMMSNNYFPRWSPDGEWFAYVHAGGTSQGAVTA
                     ELMLVASAGGSPKKLQLASHRVGTQDNVADLASSMPSWAPHNTLAPPPERDWISFVSA
                     RPYGAVLPTAGRGQIWVTSVDLSTPGDPSSAAFWLPCQDATVLNNNPVWTKTEVAE"
     gene            7047..7988
                     /locus_tag="VFV99_32820"
     CDS             7047..7988
                     /locus_tag="VFV99_32820"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5064219.1"
                     /translation="MMRLIPLLLVLALATPAHAGSVGIVVTGDSEMQDGLELQLEGWL
                     KSHGHKVGAGLPDDAKSSLLNCMVIDDEACARGIVDSRSKSDSVVFAEIRKPRTRASN
                     ATTLIVLWLVKGKEPVGMRKSCEDCNPDLLDSMLDEVLTTVVGASELARGRLELHSKP
                     EGVTVMLDNQNVGITPLEREVAAGSHTIVLMSKGRKVGERTLKLQPEMTAEITMPVVI
                     PPDEDRPSKVLPGVILAVGGIAIATGAVLYFTSEVDDGTKPMYRDSKPPAIGIAAGGV
                     ALAAIGTYLWFRTGTSDSTPVAAIDQHGGYIGWARAF"
     gene            7970..9226
                     /locus_tag="VFV99_32825"
     CDS             7970..9226
                     /locus_tag="VFV99_32825"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5064220.1"
                     /translation="MGARVLILILALAACEKQSKLYCEKHPGDLDNCGFLDAGIDARP
                     MCASDMDCAATPSAPYCELNIHECVECYQSGQCTANPVEKFCDLSTFQCTSCVRNTDC
                     TSNVCLPNGECGDDSNVAYVDPDLGGNNATCAMATPCKTLAAALTTKKPFVMLKGMLD
                     ETVTIDAQSVTILAEPGTVLTKSSNGVVLTITGSSDVSIFDLTIVGAAEKGIALDMNS
                     TLRVNRVTITGCDGKDRRAIEAKNATLFVSRSTIYSNLGGGVYVDAGSIYQITNSFFY
                     RNGDVGSATGGLTLLPTSSALNRFEMNTVVDNHAKATVPGGVSCSANIAAPNNLVVHN
                     TSGTILLDTTQIQVGGCNFDDSHYGGDLTEYMFKVPDGTGPWDYHVNAGSMAIDRGVT
                     SDIKFDLDGETRPQGTQNDLGADEFK"
     gene            9260..10075
                     /locus_tag="VFV99_32830"
     CDS             9260..10075
                     /locus_tag="VFV99_32830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064221.1"
                     /translation="MRGLLLVYLVARVASAEPAPPPHLAAYKADAKAEAIARALWDDY
                     GDVATVGAEEDMDGGYRGTIHLVPELPINKYRTQLEWVAAAMKDIDAFFSAFDTKPSY
                     RWKQLTFQFVRSVKKRTPSAYATGWAITHNVAGSLLTSARGVRETYFHELFHLNDFDH
                     NDWSQKTLGKDYAAIVKKCGIKVKCLDPYAPNTTKVRATGTYYAFQPNNGNGVHEYAA
                     ELAVRYFKEQTEMQTNRRLGQKAFKCGPPENARAWQALVDEFFAGRDLVPPCS"
     gene            10306..12015
                     /locus_tag="VFV99_32835"
     CDS             10306..12015
                     /locus_tag="VFV99_32835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064222.1"
                     /translation="MMDPPQAASGRGGDAYDSGLDDEGDDNGLVISTGRDILVVDDND
                     TNLIAIEAALQPLGRKMVLARSGVEALARLLEQDFALIILDVAMPGMDGFETARLVRS
                     RARNRATPIIFITGLSWQDDEILRGYELGAFDFLMKPIRPEVLRAKATVFIELQQRTL
                     ELSQKADELRQEQARAHERELTMQRRRFEAEVLERQMAQMADADRRKDEFLAILAHEL
                     RNPLQPLQTAVELMEHNPSEPVPERVRGIIQRQVHHIGRLVDDLLDVARFQTGKLDLR
                     REPLALDAIVSEAVNDSKPSADSRQQNVSVKGEGAPPIVYGDTLRLVQVLTNLLSNAI
                     KYTNPGGTIDVRWGTLGGHGFVKVTDNGRGIPGPLLPRIFDMFVQERTTPDGKGGLGL
                     GLGLVKRLVELHQGSVHASSPGPDKGATFEVRLPLADDETIAKFQPPRTKTGPNERPL
                     RAVVCDDAADLRELVADLLRMKGHEVTVVEDGPSAVRVITAEKPDVALIDIGLPDMDG
                     YEVARTIRRDMTHRVRLIAMTGYGQAADRATALQAGFDAHIVKPASADKIMRALFGQN
                     EET"
     gene            12012..>14585
                     /locus_tag="VFV99_32840"
     CDS             12012..>14585
                     /locus_tag="VFV99_32840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5064223.1"
                     /translation="MSTVESPKTNGNGSGTKTTNGKSSGGKVVRAHGADQLNHRELLA
                     ALRAFKRGNFDVKLREDLTGIDGQIVETFNELVSMVKAIRDESHEVCDAVGKEGQAAK
                     RMRRFGTTGGWSEYIASVNSVIEDLSGHANEIARVVSAVAKGDLEQTMDTDDGGHGPR
                     RGEFLRHAKIVNGMVARLAQFGSEVTRVALEVGGEGKLGAQARVQGVSGVWKDLTDSV
                     NLMASNLTSQVREVARVTTAIAQGDLSKTVTIDVKGEILELKNTINTMVEQLRGFANE
                     VTRVAREVGTEGRLGGQATVRGVSGVWRELTESVNSMANNLTFQVRNIAEVTSAVASG
                     DLGKKITVEAKGEILGLKNTINTMVDQLSAFAAEVTRVAREVGTEGVLGGQAEVADVS
                     GVWRELTQNVNSMASNLTSQVRNIAEVTAAIAAGDLSKKIGVDARGEILALKNTINST
                     VDKLNRFSAEVTRVARLVGTEGTLGVTADVKDVSGIWKELTDNVNQMARNLTGQVRDI
                     AEVTTAVANGDLTRKITVEARGELVTLKNTINTMVDQLNAFASEVTRVAREVGTEGVL
                     GGQAQVRDVSGIWKELTDNVNSLASNLTNQVRNIADVARAVATGDLSRKITVDAKGEV
                     LDLKATLNTMVDQLNAFASEVTRVAREVGTEGRLGGQASVEGVRGTWKELTDNVNQMA
                     SNLTGQVRDIAEVTTAVARGDLTRKITVDAKGEILELKNTVNIMVDQLSSFADQVTRL
                     ARDVGIEGRLGGQADVRGVLGTWRNLTDAVNSMATNLTDQVRNIAKVATAIAAGDLQQ
                     KIVVEARGEILELKNTVNTMVDQLSAFADQVSRVAREVGTEGKLGGQASVEGVRGTWK
                     EL"
BASE COUNT         2621 a         4721 c         4802 g         2441 t
ORIGIN      
        1 ggtacacgtc ccggccgcgc tcgatgcggc cgccgcggcc acactcgtgc tcgagcgcgt
       61 agtcgccgac ggccgtcccg atctcgccga agtcgacctt ggcggcgacc tcgcgcatga
      121 atgcgcgaaa gtcgtccggt atcttgaacg actgactcac ccgtcgacga tgcgagcaca
      181 cgacggtgtg ccaacgcaag tcgatatcgc tcgtaggcgt aagcgcaagc gtaagcgtaa
      241 gcgaatctgt gactgcgctt tcacgagcgt caccgagagt acgtcctcgc aagagagagc
      301 cgcgaaccgt gatgcagttg cgacgatacg ccgaggtgcg attgtttcga cgccagcaaa
      361 acaccgtcgc gctccttcgc gtcccggatc gctgcacgga cagcggcgac ccatcgatca
      421 gatcgcgatc ggatcgccgc ttacgctcac gtggtcgcta catgccgacc gaatacacga
      481 agctcggctt gggatggcgc agcatgcgct ccgaaattag cttgtgcggc acgacggtct
      541 cgctgtcgac gtacttcgcg atcagcttct cggccgccgc cttgtcgccg cgcgccttga
      601 tgccggcgac ttctttcatc agatcgttcg cgaccggcac gagcttgtca gcgtggatcg
      661 tgaacgcgcc cttgtcggtg ccgttcgccg ccgtcgcgtt tgcatcccag gtcaatgcgc
      721 ccttctcgat cagatagccg acctgaatcg cggcgagatt gccgtacgcc ttgcggttgt
      781 gctcgccggt gtacatgccc tgcgagatgt gaccgaacgc ccagatgatc gaatcgacga
      841 acgattgctg cgccatctcg tcggagatca gcttcttctg gcgcaagaag tcgagcaggt
      901 atagcgcacc gctctgcgcc ttgagctctt ccatcatcga cgcgatcggg ccggtgaaga
      961 tgacgtcgtc gtccttgccg ttcaccttgt actcgtgcgc ggggccgagg ttgtgcgtcg
     1021 cctcgtgcag gatcgtcgac agcaggcccg gctccgtcga ctccgggtag agcttggcgc
     1081 tcgcggcatc gagcaggctt tcggcttgct gcttgcgtgc cgcgcggctg tccggatcgg
     1141 tgttgatgtt gaccatcgcg acggtgcggc cgcgcccctc gttcgcgacc gggccccagt
     1201 tcggcaggct ctgaccgatc gtagcgccga gcggcgcgcg gtcctcgccc gcgttgatga
     1261 tgatgtcgat gaaatcgggc aagtggaacg tgaccttgcg cgccttgtac ggcgcaccgg
     1321 cgaccttcgc gatcgcgtcc tccatctcct gctgcaccgg cacgagcctc gcctgccact
     1381 ccttgctgcc ttggttgatg cgcgcgaacg acaggtgaaa gcctgccttc tgtgcgcacg
     1441 gttcccagta cgtctcgtcg ggagcgacgc gcacgtacca cttcgagttg tcgacgctca
     1501 tctttgccca cgcctcgtcg gcggggaccc agtcgttgga gccgaagctc gcggccgcgg
     1561 ccttgaggta cgcgacgagc ggctgctcgt tcggatcctt gatgccgttc gcggtctccg
     1621 tgagcgcgtt cgcgatcgcg gtcatcttgt ctttgtaggc ctcggtgtac ggcaccggct
     1681 tgagcgcatc gccttcgcca cgaaccacgt tgaagtggcc catgagcgcc tcgccgtcct
     1741 tgcgcgcctg gaggtccttg cagaacgtgt ccttcgtctg gatcgcggcc ggatagaggt
     1801 cgaccgggac ctttggcgcg cccggcagcg gcgagcacgc cggatccttg ctcgacaccg
     1861 ggccttcaca cttcgcgcct cggttgcggc ggaacaagct gcggctctcc ggatccgccg
     1921 gcagctgtgc gaccacggcc ttcgacccgt tcgtggtctc gaagaggtcg tcgatctgat
     1981 caccgatcgc gagcatgcgc ttcacgaacg tcttgtcgtc ggcagataac gtcgagaagt
     2041 cgctgcgcac gagcgtcgcg cggccggcgt cgagatcctg gcgaacgagc gcgcgccgct
     2101 taccgtcctc ggtgctggtg tcgagcggcg gctctttcga cgccgccacg atcttgttat
     2161 aggccgcttc gaagtccgcg gtgaacttgc cgtccgcggt ccacgtcggc gacgtgccgt
     2221 agaacaacag aggtgcgact tcgtcggcgt cgagcacctt gttgttgttg ccgtcgacga
     2281 tccagtagac cgggatgttg aggcgcacgg cgaatcgatt gaaatcggga cgcgcgatct
     2341 ggtcgtgtgc cgcagccgca gccgccgagc ccgagccagt cttcggcgcc tcgcgcggtt
     2401 ggggttcttg ccgtcgcttg caggaaacag ccaggagcgt gaggagaacg agcgtccagg
     2461 tacgcatggc gcgaacgcta ccatggtggt ggtaccgtcg ctgtgcgtgc ggggttgcat
     2521 caacgcgctc gtgcttgccg tcgcagccgt cgcagctgtc gcagcggacg ctggtgcgcg
     2581 ctccggcgag cccatcgtgt tcgtcggagc tgaccccgca ttctcgagcg cgctcgatga
     2641 cgcgctattg cccgccggca tggacgtgtt gccgatcggc atcttgccgg caccggcgct
     2701 cgccgaattc gcagcacgct cgcgcgagct tgccgaccgc gagcacgcga ccgcgacggt
     2761 gtggctgttg cccgcgcaga gtggcgcaac gctcgtcgcg tatgatcgcg gcgtcgatcg
     2821 cttgctcgtg cgcgagctgc cgtatcgctt gccgctgtcg gcgacgcagg ctgccgaggc
     2881 cgcgcgcatg gtgcgcacga tgttgcgtgc gctgcgcgtg acacccgata gcgagctcgc
     2941 gccaccaccg gtcgcgccaa ccggtccacc gcagccgctg atcgccgcga gtcttggcat
     3001 cggcgcatgg ttcgcagcac ccggcaccga cagcgcgctc gccggaagct tgacggtcgc
     3061 gtggcggccg catggcctgg gcgccgcact caccggcatg ctcgcgccca gcgctgacgt
     3121 catgagcgtg acgttcgagg gccggatcag cgacatcgtg attgctgcgg aagcgcgcaa
     3181 agcgctgctg ctcccgcccg cgatccgggt gaccccggca gccgggatcg cgctgcatct
     3241 gctccggctg accgggtcgc ttgggggcgg cagcgagctg tcgtcccgcc gctacgaccc
     3301 tgccgcgcgg ctcggcgtaa ctgctggtta tgccctgcct cgtggcctcg aggtcggcct
     3361 cagcgtgtcg gcggattgcc tactccagcg ccagcgctac caggtcgcaa gtgaggagat
     3421 tctggtcatt cctcgccttc aggtcgtgac cgggatcgtc gtcgggttga gactctaagg
     3481 aggagcgcga cttatttcgg ggaagcagcc actagatggc gtgggcctgt ctatcgtcga
     3541 acgcccgacg cccgaggacg acgcggagct tgttgccgcg tgcgttgccg gggacatggc
     3601 ggcccagcgc gcgctgtttc gccgcgagta tccgcgggtc aacgcgacgg tctaccggct
     3661 catcggctcc gcgcgcgatg ccgatgacct cgtgcaggag accttcatcg ccgtgtttcg
     3721 cggcctcccg cggttccgcg gcgacagcaa gcttgcgacc tggatcgatc ggatcgccgt
     3781 gcgcgtcgtg ttccatcata ttcgcgaccg cggccgcgcg ccgattccac tcgacgtgat
     3841 cggtgacgtc gaaggcaacg gtggctcgac cgatgcgcag gcacatgcgc gcgatgggct
     3901 acgtcgtttc tatgccgcgc tcgccgagct cacgcccgaa gcgcgcaccg cgttcgcgtt
     3961 gttcgcgatc gacggtcgtt cgatctcgga ggtcgcgcag gtgacgcaga cctcgttcgt
     4021 cgcagcgaag tgccgaatct ggcgcgcacg ccgcgaaatc atgaagaagg caaaggacga
     4081 tccggtgttg gcagagctcg taaaggagga ccgatgaagc gcatcgtcgt cgatcctcca
     4141 gtcagtgaag cacgtcgcga gcgcgtcgag cgtcagctgt tccagcagct agccgccgtc
     4201 cgcgtcgctg atcgcgcaga ggcagtcatt ccgccgccga agaagagccg cgcagcgatg
     4261 tggtgggggg ccgcggcatg cgctgcagcg gtggttgcaa ccgttctgtt catcacacgc
     4321 gacggtgcga cagggggaca gcttgcgcag ccgtcgcgtg tggtgactcc tgtcgatagc
     4381 gagagcacgt ttacgatcgg ccacgaagct gtggtcgtcg cgaagcgcga taccagtgtc
     4441 gagtggcacc agggccgcga tggctcgatc acgttgcagc tcgcgcgcgg cgccgtcgat
     4501 tgccaggtcg agccgcgtca cggtcgcccg ccgttcaagg tggtcagcgg cgacatcacc
     4561 gtcgttgttg tcggtacgcg cttcacggtc actcgcacgc cgagcccgcg cgtcgatgtc
     4621 gcgcacggca aggttcgcgt cgaagcaccg ggtggcacgt ggttcgtcga ggccggtgag
     4681 tcgtggacgc cgatttcggt gaccgcgatc gaagaacccg atcaggcttc ggaccccggg
     4741 cttcaggctt cgggtcctgc gccagagatc gagatggatc cgatcaaggt gtcgcgcgcg
     4801 aagaaggcgg cggcgccgcc ggcgcccgag tccgactctg gccgaagcct gaagcgcgag
     4861 gcccgaagcc cgtccagccc gatcgcgccg cccgagacgc gcgagctcgc gaaagaggcg
     4921 taccgcgccg cgcagcacct cgagaacacg gaccccaagc tcgccgccgc gaattaccgc
     4981 gcgatcgcgc tgggcaagga tgatggttgg gctgcgaccg cgctggtcgc gctcgccgag
     5041 ctcgacctca agcagaacga gccgcaagcg gcgctcgatg cgctcgacga gcatgcgcgt
     5101 cggttcccgc gctccgcgat gcgcgaggac gcggcctggt tccgcatcga ggcgctacgt
     5161 tcgatgggta agcgtgacga agcccgcggt gcagccgccg attatctgcg cgagttccca
     5221 gacggcgtct acgtgaagcc cgccgcacgc ttggtcaagt aacagcgtga cgaacgacgc
     5281 ggcgtagccg cgtatactct gagacagttc tcagcaggca cctagtgcct gtgcctggct
     5341 atgcttcgac taacgatgtt cctgttggct ctcgtggggt gcaccgagac ggtgcatctg
     5401 tcgcgcgatc cactcgaggg cctcgtgtcc ctcgagctca cgccgttcga ttcgacgatc
     5461 aagatcaccg acatcgcgcc gccacatcac acgctgcaat tcttggcgat ggggcacttc
     5521 gaagacggca cgacgcgtga catcaccgtg ctcgtcgagt gggcgatcga caatgccctg
     5581 ctcggcacgt tcgacacgcg cggcctgttc accgcgtcgc atgccgccgc tggccacggc
     5641 aacgtcacga tcgaagcacg cggcctgcag gcgacgacgc agctcaccgt gatcatcgac
     5701 gcgacggtga tcgatccggc gttcccaccg ccgggcgcga acctgttcga tccatcgatt
     5761 ccgttcacga ccgatccgat gcgatcaccg acgctgacct acccggccga aaccacgacg
     5821 ttcccgcaga acatcgcgag cacgttgttc cagtacgagc gcggcaccgg caacgacgcg
     5881 ttccgcatcg tgtttgattc cgaagtcatt cacctcgcgg tcgagaccgg ctccgatcgc
     5941 tggcaggcag acggtgacat gcagcggctg ctcgccgcga ccggaatcac cgcgccgatc
     6001 cgcaccgaga ttcaggcgcg cgcgtcgacc atgatgccgg cgatgattta cgtcggcaac
     6061 cacgcgacgc tcacgttcac gaaggatgcg cccggcggag cgctgctgtt ctggtcagcg
     6121 gcgaccaacg gcatcatgcg tggtggcgtc gagatgcagt cggccggcaa gctctaccca
     6181 gcatcgacga cgtgcgtcgg ctgccatgcg gtgtcacgcg acggtgcgca actcgcgatg
     6241 ggcgtcgaca acatgccgac gaccgacttg ctcacggtcg atatgggctc gctctcgccg
     6301 cgcattccac agagcccggc gcggccgatg ggctgggcga cctactcgcc cgacggcacg
     6361 cggctcatcg tcgcgaacaa cggcgtcctc acgcagtacg acagtgcgag cgggatgtcc
     6421 ctcggcaccg tgccgcttcc agcgatgcgt tatgcgaccc atcccgactg gtcacccgat
     6481 ggccaatgga tcgtcgtcgc gatgacgagc acagcgccga cgaacatgga cgtcaaatcc
     6541 gcgagcatcg cgaagattcc gttcaacgac ggcgcgtggg gaacgccgca gatcatcgtg
     6601 tcgggctcga tgatgagcaa caactacttc ccgcgctggt cgcccgacgg cgaatggttc
     6661 gcgtacgtgc acgcaggcgg gacatcgcaa ggcgccgtga ccgcagagct gatgctcgtc
     6721 gcctccgccg gtggctcgcc gaagaagctg cagctcgcga gtcaccgcgt cggcacgcag
     6781 gacaatgttg ccgatctcgc gagctcgatg ccgtcgtggg caccacacaa cacgcttgcg
     6841 ccgcctcccg agcgcgactg gatctcgttc gtatcggcgc ggccgtacgg cgcggtattg
     6901 ccgacagcgg gccgcgggca gatctgggtg acctcagtcg atctctccac gccaggcgat
     6961 ccatcgagcg cggctttttg gttaccgtgt caggacgcga ccgtgctgaa caacaacccg
     7021 gtatggacga agaccgaggt cgccgaatga tgcgcctgat accgttgttg ctcgtcctcg
     7081 cgctcgcgac gcctgcgcat gccggttctg ttggcatcgt cgtcaccggc gactcggaaa
     7141 tgcaggacgg tctcgagctc cagctcgagg gctggctgaa gtcgcacggc cacaaggtcg
     7201 gcgccgggct gccggacgat gcgaagagct cgctcttgaa ttgcatggtg atcgacgacg
     7261 aggcgtgtgc acgcggcatc gtcgattcgc gctcgaagag cgacagcgtg gtgttcgccg
     7321 agatccgtaa gccgcgcacg cgcgcgagca acgcgacgac gctcatcgtc ctctggctcg
     7381 tcaaaggcaa ggagccggtg ggcatgcgga aatcgtgcga ggactgcaac ccagacctcc
     7441 tggacagcat gctcgacgag gtgctgacca cggtcgttgg tgcgagcgag ctcgcgcgcg
     7501 gccggctcga gctgcactcc aagcccgaag gcgtgaccgt gatgctcgac aaccagaacg
     7561 tcgggatcac gccgctcgag cgcgaagtgg ccgctggatc gcacaccatc gtgctgatgt
     7621 cgaaggggcg gaaggtcggt gaacgcacgc tgaagctgca gcccgagatg acggccgaaa
     7681 tcacgatgcc ggtcgtgatc ccgcccgacg aggatcggcc gtcgaaggtg ctccccggcg
     7741 tcatcctcgc ggtcggcggc atcgcgatcg cgaccggcgc ggtgctctac ttcacgagcg
     7801 aggtcgatga cggcacgaag ccgatgtatc gagacagtaa gccaccagcg atcggcatcg
     7861 ccgcgggcgg tgtcgcgctc gcggcgatcg gcacgtatct gtggttccgc accggcacca
     7921 gcgactcgac accggtcgct gccatcgacc aacacggagg atacatagga tgggcgcgcg
     7981 cgttctgatc ctcatcctcg cgctcgctgc gtgcgagaag cagagcaagc tgtattgcga
     8041 gaagcatccc ggcgatctcg acaactgcgg ctttctcgac gccggcatcg acgcacggcc
     8101 gatgtgcgcg agcgacatgg actgcgctgc cacgccgagc gcgccgtact gcgagctcaa
     8161 catccacgaa tgtgtcgagt gctatcagtc cggccagtgc acggcgaatc ccgtcgagaa
     8221 gttctgcgac ctgtcgacgt ttcagtgcac gagctgcgtg aggaacaccg actgcacgtc
     8281 gaacgtgtgc ttgccgaacg gtgaatgtgg cgacgacagc aatgtggcct atgtcgaccc
     8341 cgatctgggc gggaacaacg caacgtgcgc gatggccacg ccctgcaaga cgctcgctgc
     8401 cgcgctgacg acgaagaagc cgttcgtcat gctcaagggc atgctcgacg agacggtcac
     8461 gatcgacgcg cagtcggtaa cgatccttgc cgagcccggg accgtgctga cgaaatcatc
     8521 gaacggcgtg gtcctcacga tcacaggcag cagcgatgtc tcgatcttcg atctcacgat
     8581 cgtcggcgcc gctgaaaaag gcatcgcgct cgacatgaac agcacgctgc gcgtgaaccg
     8641 ggtgacgatc accggctgcg acgggaagga ccgccgcgcg atcgaggcga agaacgcgac
     8701 gctgttcgtt tcgcgctcga cgatctactc gaacctcggc ggcggcgtgt acgtcgacgc
     8761 cggctcgatc taccagatca cgaactcgtt cttctatcgc aatggcgacg tcggttccgc
     8821 gaccggtggg ctgacgcttc tgccgacgag ctccgcactc aaccgcttcg agatgaacac
     8881 ggtcgtcgac aaccatgcga aggcgaccgt gcccggcggc gtgagctgct cggctaacat
     8941 cgcggcgcca aacaacctcg tggtccacaa cacgtccggc acgatcctgc tcgacacgac
     9001 acagatccag gtcggcggtt gcaacttcga cgactcacac tacggcggcg atctcaccga
     9061 gtacatgttc aaggtccccg acggcacggg gccgtgggac taccacgtga acgccggcag
     9121 catggccatc gaccgcggcg tgacgagcga catcaagttc gacctcgacg gcgagacccg
     9181 cccacagggt acgcagaacg acctcggcgc cgacgaattc aagtagagcg tggcgatccg
     9241 ccgtgtcacg ctagtcgcga tgcgcgggtt gttgctggtc tatctcgtcg cgcgcgtggc
     9301 tagcgcggag cctgcgccgc cgccgcatct cgccgcgtac aaggccgatg cgaaggccga
     9361 agcgatcgca cgcgcgctgt gggacgacta cggcgacgtc gcgaccgtcg gcgccgaaga
     9421 agacatggac ggtggctatc gcggcacgat ccacctcgtc cccgagctac cgatcaacaa
     9481 gtacagaacg cagctcgagt gggtcgccgc ggcgatgaag gacatcgacg cgttcttctc
     9541 ggcgttcgac acgaagccga gttatcgctg gaagcagctg acgtttcagt tcgtgcgctc
     9601 ggtgaagaag cgaacgccga gcgcctacgc gacgggttgg gcaatcacac acaacgtcgc
     9661 cggctcgctg ctcacgagtg cgcgtggcgt acgcgagacg tactttcacg agctgtttca
     9721 tctgaacgat ttcgatcaca acgactggtc gcagaagacg ctcggcaagg actacgcggc
     9781 gatcgtcaag aagtgcggca tcaaggtgaa gtgcctcgac ccgtacgcgc cgaacaccac
     9841 gaaggtccgc gcgacgggca cctactacgc gttccaaccg aacaatggca acggcgtgca
     9901 cgagtacgca gccgagctcg cggtccgcta cttcaaggag cagaccgaga tgcagacgaa
     9961 ccgcaggttg gggcagaagg cgttcaaatg cggaccgcca gaaaatgcgc gcgcgtggca
    10021 ggcgctcgtc gacgaattct ttgcgggccg cgatctcgtg cccccctgta gctaaccccg
    10081 cgatcagaga aacgagcgct cccgccacat gcgtgcgcta ctgcgctcgg tgtgcgcatg
    10141 cgctcgtttt ggctctcgaa gtcgctcgaa gtactggaga ggacatacgt aatccgggca
    10201 catgccatgc agaacatgcg gggtccgggg tatggctgtc gggtcgttac gcagtggaga
    10261 agtttggctt ttccaacggg agtaaatgga accacggtgc acatgatgat ggatcctccg
    10321 caagcggcct cgggtagagg tggcgacgcc tacgactcgg ggctcgacga cgaaggtgat
    10381 gacaacggcc tcgtgatctc cacgggtcgc gacatcctcg tcgtcgacga taacgacacg
    10441 aacctgatcg cgatcgaagc ggcgctgcag ccgctcggtc gcaagatggt gctcgcgcgt
    10501 tcgggtgtcg aagcgctcgc gcgcctgctc gagcaggatt tcgccctcat cattctcgac
    10561 gtcgcaatgc ccgggatgga cggcttcgag accgcgcgtc tcgtccgctc gcgtgcgcgt
    10621 aatcgtgcga cgccgatcat cttcatcacg ggcttgtcgt ggcaggacga cgaaatcctt
    10681 cgtggctacg agctcggcgc gttcgacttc ttgatgaagc cgatccgtcc ggaagtgctg
    10741 cgtgcgaagg ccacggtgtt catcgaattg cagcagcgca cactcgagct ctcgcagaag
    10801 gcggacgagc tgcgtcagga gcaagcgcgc gcgcacgagc gcgagctgac gatgcagcgc
    10861 cgccgcttcg aagccgaggt gctggaacgt cagatggcgc agatggccga cgccgatcgc
    10921 cgcaaggatg agttcctcgc gatcctcgcc cacgagctgc gcaacccgct ccagccgcta
    10981 cagaccgccg tcgagctgat ggagcacaac ccgagcgagc cggtacccga gcgcgtgcgc
    11041 ggcatcatcc agcgccaggt ccaccacatc ggccgactcg tcgacgatct gctcgacgtc
    11101 gctcgcttcc agaccggcaa gctcgatctg cgccgcgaac cgctcgcgct cgacgcgatc
    11161 gtcagcgaag ccgtcaacga ctcgaagcca agcgccgact cgcgccagca gaacgtgtcc
    11221 gtgaagggag aaggcgcgcc accgatcgtt tacggcgaca cgctgcgact cgtgcaggtg
    11281 ctgacaaatc tattatcaaa cgcgatcaag tacacgaacc ccggcggcac gatcgacgtg
    11341 cgctggggca ccctcggcgg tcacgggttc gtgaaggtca ccgacaacgg ccgcggtatt
    11401 cccggtccgc tgctcccgcg gatcttcgac atgttcgtgc aggagcggac gacgcccgac
    11461 ggcaagggcg gcctcggcct cggcctcggt ctcgtcaagc gcctcgtcga gctgcaccag
    11521 ggcagcgtgc acgcgtcgtc gccaggcccg gacaagggcg cgacgttcga ggtgcggttg
    11581 ccgctcgcgg atgacgagac gatcgcgaag ttccagccgc cgcgcacgaa gaccggtccg
    11641 aacgagcggc cgctgcgcgc ggtcgtgtgt gacgacgccg ccgacctccg cgagctcgtc
    11701 gccgatttgc ttcgcatgaa gggccacgaa gtcaccgtcg tcgaagacgg accgtcggcc
    11761 gttcgcgtga ttaccgccga gaaacccgac gtcgcactga tcgacatcgg gcttcccgac
    11821 atggatggct acgaagtcgc gcgcacgatc cggcgcgaca tgacgcaccg agttcggttg
    11881 atcgcgatga ccggttacgg ccaagcggct gaccgagcaa ctgcactgca agcgggcttc
    11941 gatgcccaca tcgtcaaacc ggcgagcgcc gacaagatca tgcgtgcgct cttcggccag
    12001 aacgaggaaa catgagcacc gtcgaatcac cgaagaccaa cggtaacggc agcggcacga
    12061 aaacgacgaa cgggaagagt agcgggggaa aggtggtcag ggcgcacggc gccgatcagc
    12121 tgaaccaccg cgagctcctt gccgcgttgc gcgcgttcaa gcgcggcaac ttcgacgtca
    12181 agctgcgcga agacctgacc ggcatcgacg gccagatcgt cgagacgttc aacgagctcg
    12241 tgtcgatggt aaaggccatc cgcgacgagt cgcatgaagt gtgtgacgcc gtcggtaagg
    12301 aaggtcaggc ggccaagcgc atgcgtcgct tcggtacgac cggcgggtgg tccgagtaca
    12361 tcgcgtccgt caactcggtg atcgaagacc tctccggtca cgcgaacgag atcgcgcgcg
    12421 tcgtgtcggc cgtcgccaag ggcgacctcg agcaaacgat ggataccgac gacggcggtc
    12481 atggtccccg ccgcggcgaa ttccttcgtc acgcgaagat cgtcaacggc atggtggcgc
    12541 gactggccca gttcggctcc gaggtgacgc gtgtcgcgct cgaagtcggt ggcgaaggca
    12601 agctcggtgc gcaggcgcgc gtgcaaggcg tcagcggcgt ctggaaggat ctcaccgact
    12661 cggtgaacct gatggcgtcg aacctgacct cgcaggtgcg cgaggtggcg cgcgtgacga
    12721 cggcgatcgc gcagggcgac ctgtcgaaga ccgtcacgat cgacgtcaaa ggcgaaatcc
    12781 tcgagctgaa gaacacgatc aacacgatgg tcgagcagct ccgcggtttc gcgaacgagg
    12841 tgacgcgcgt cgcccgcgaa gtcggtaccg aaggccgcct cggcggtcag gcgaccgtcc
    12901 gcggcgtgtc gggcgtgtgg cgcgagctca ccgagtcggt gaactcgatg gcgaacaacc
    12961 tgacgttcca ggtccgcaac atcgccgaag tgacgagcgc ggttgcgtcc ggtgacctcg
    13021 gcaagaagat caccgtcgag gcgaagggcg agattctcgg tctcaagaac acgatcaaca
    13081 cgatggtcga tcagctcagc gcattcgccg ccgaggtgac ccgcgttgct cgcgaagtcg
    13141 gtaccgaagg tgtcctcggc ggtcaggccg aggttgccga cgtttcgggt gtgtggcgcg
    13201 agctgaccca gaacgtgaac tcgatggcgt cgaacctgac gtcgcaggtg cgcaacattg
    13261 ccgaggtgac cgcagcgatc gctgccggcg acctgtcgaa gaagatcggc gttgacgcgc
    13321 gcggtgagat cctcgcgctg aagaacacca tcaacagcac ggtcgacaag ctgaaccgat
    13381 tctccgccga ggtgactcgc gtcgcgcgtc tcgtcggtac ggaaggcacg ctcggtgtga
    13441 ccgccgacgt caaggacgtg tcgggcatct ggaaggagct gaccgacaac gtcaaccaga
    13501 tggcgcgtaa cctgaccggt caggtccgcg acatcgccga ggtgacgact gccgtcgcga
    13561 acggcgacct cacccgcaag atcacagtcg aagcgcgcgg cgagctcgtc acgctgaaaa
    13621 acacgatcaa cacgatggtc gatcagctga acgcgttcgc gtccgaggtg acgcgcgtcg
    13681 cgcgcgaagt cggtacggaa ggcgtgctcg gcggtcaggc ccaggtccgt gacgtgtcgg
    13741 gcatctggaa ggagctgacc gacaacgtca actcgttggc gtcgaacctc acgaaccagg
    13801 tgcgcaacat cgccgacgtt gctcgcgcgg tcgcgaccgg cgatctgtct cgcaagatca
    13861 ccgtcgacgc gaagggcgag gtgctcgacc tcaaggccac gctgaacacg atggtcgatc
    13921 agctgaacgc gttcgcctcc gaggtgacac gcgtcgcgcg cgaggtcggt accgaaggcc
    13981 gtctcggcgg tcaggcgagt gtcgaaggcg ttcgcggtac gtggaaagag ctgaccgaca
    14041 acgtcaacca gatggcgtcg aacctgaccg gtcaggtccg cgacatcgcg gaagtgacga
    14101 cggccgtcgc acgaggtgac ctgacccgca agatcaccgt cgacgcgaag ggcgagattc
    14161 tcgagctgaa gaacaccgtc aacatcatgg tcgatcagct gtcgagcttc gccgatcagg
    14221 tgacgcgcct cgcgcgtgac gtcggtatcg aaggccgcct cggcggtcag gccgacgtgc
    14281 gcggtgtcct gggtacgtgg cgcaacctga ccgacgccgt gaactcgatg gcgacgaacc
    14341 tgaccgacca ggtgcgtaac atcgcaaagg tcgcgaccgc gatcgctgca ggcgacctgc
    14401 agcagaagat cgtcgtcgag gcgcgcggcg agatcctcga gctgaagaac accgtcaaca
    14461 cgatggtcga tcagctcagc gcattcgccg accaggtgtc gcgcgtcgct cgcgaagttg
    14521 gtaccgaagg caagctcggt ggccaggcct ccgtcgaagg cgtccgcggt acgtggaagg
    14581 agctg
//
LOCUS       DASUFX010000811         5891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1242517, whole genome shotgun sequence.
ACCESSION   DASUFX010000811 DASUFX010000000
VERSION     DASUFX010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242517"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..2007)
                     /locus_tag="VFV99_32845"
     CDS             complement(400..2007)
                     /locus_tag="VFV99_32845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064224.1"
                     /translation="MAEESFAAGLVGVVLDDRYRLDALLGEGGMGAVFRAHHLAMDRK
                     VAVKLLKPHLTTDEAALQRFAREARATMRVESDHAVKVLDFGVTPHKDYYMVLEYLDG
                     RTVQRELDVDGPFDPGRVVHIAKQACAALGAAHRSGLIHRDVKPDNLLLMRSGDDPDF
                     VKVLDFGVAKLMEGAARSDRSMLAITQAGMVFGTPEFMSPEQACGQQLDGRSDLYSLA
                     ATMFAMLTGCGMYRAKSAIEWLTHHARTPAPHLADGAPSLAQYTELDAVLQRCLAKQR
                     DARPQTAEQMAQMLASVEHSLGKVRAPLPEMRGPVFSPSTYVTALDPNATLLPQQVSP
                     KASAAAATMKPGDGMMMPGQSIKIGTLPPSGTDAIEMPQPKRRGMWLVVGAVIVAGMI
                     AAAIVVAMKKRSPTTPSPVAKAADKPVDAEIADAAVADAPAIAVDAAVPVDAGVQITR
                     KGSNPEIAKLIAEAEEAKRGRKLVRWVQKADAALQLDSRNVKARFLLAEGLIASGDLD
                     RGCKYLRELGRNPTAKKLATQAGCPAD"
     gene            complement(2012..2203)
                     /locus_tag="VFV99_32850"
     CDS             complement(2012..2203)
                     /locus_tag="VFV99_32850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064225.1"
                     /translation="MSSLVQRNKVVAKRKGTLAAATAIGAGVAAVAGAPVVGVIAAAG
                     AAYLAWDWFSFRVKNGMRF"
     gene            2416..3825
                     /locus_tag="VFV99_32855"
     CDS             2416..3825
                     /locus_tag="VFV99_32855"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5064226.1"
                     /translation="MSPNGLGQRDAVLFGLAGGMLVVGAALAGGYYVISTRSEARAAT
                     RPPVVEARIDAVTHSPGASGGAALDAFPSKVVEVKTGGGIAKKTWAELGIEIDRDELG
                     RAGLVNSEQQLAALAKKGSIPVRIDRDKAVKAIIGLKGQYDKAAIDAHLDLEARKIES
                     DSPGEGIDVWASLPKIVSAARSGVTSFELVSVAVPARVTKQTLGIDDISNVLGQYSTK
                     FAVTDKDRNFNLKLAASHINGAVLQPGVEWSFNGTVGERSQATGYKIAHVITAGEMVD
                     GLAGGTCQISTTLFGASFFAGLDIVKTLNHSRPSAYTPLGFDATVVWPDTDLVLRNPY
                     DFPVAVLYRVANGEAHVEILGKKRPWTKVQFVREVIEENPYSTEERIDEEMPMGLTMT
                     DQPGFNGYKLKRYRRFFQGKKMVKENKWTVNYKPVTEYIRRGSSMDPNVVAPKEKEQH
                     LLKPVKPEEMNTEKVILTQ"
     gene            complement(3829..4578)
                     /locus_tag="VFV99_32860"
     CDS             complement(3829..4578)
                     /locus_tag="VFV99_32860"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX5064227.1"
                     /translation="MRPLYDLASVFAAQIRTAEQEANKCYEPVIGVVTDNKDPQKLGR
                     VKVKFPILSDKDTSWWCPIIMLGAGKNRGWFFIPEVDDEVLCMFEHGDLDRPVIVGAL
                     WNGKDKPADKNPGGNPRRVIKSRQGSKITFDDDQMKIIIEDGTGKGKITFDSNANKIL
                     IEAIEGDVCFQCPTGDMKVVAKTAEFKATGNVEIHAGQAMQWGTGANATIKGSGGVTM
                     SGAKVNMNCGVAQMPQAPTASPKDIDDPYGS"
     gene            complement(4592..>5891)
                     /locus_tag="VFV99_32865"
     CDS             complement(4592..>5891)
                     /locus_tag="VFV99_32865"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5064228.1"
                     /translation="FIVAADRVTDHTLAWISRQQGHKWFMWAHYIDPHGRYVAHPDVI
                     DYGASEPDLYDAELRWTDQQIGRLFDELVRLPSNDRTIIIITSDHGDSMGEHTVPVGT
                     HGTALYFELLHTPLLIYIPNNQPRTIGGAVTSLDIIPTITDILGIDTKDLSFEGRSLV
                     PQIFYGKEDHDRIVFSETNAPSPQRAAISEAWKLIFYVQSNLYELYDLKADPVEKTNL
                     APKNPPAMTTMKTMLDAWLERVLYARDTKFNQAGKQMADLVLTAAPTPEVVTQGQTLD
                     DGKLEILGVGVADKSPRPAIGSRVDLDVYFKVKERTPIAYRFLLAVWPVTTPAWKPTD
                     PAPTTMQRSPMRPTGDGFFPSDRWRTGEYIRDKFTVTIPSDWLGNGLAVGLVASEPAG
                     AKSLATGAAPANDPNLIVLGVLPLATSATPPAGSNAPNQP"
BASE COUNT          994 a         1872 c         1958 g         1067 t
ORIGIN      
        1 ttcttgatca acatgtcgcg gacgagggag ccatcgactt cctcggtcgc gatccacagc
       61 acttcgccgg tcaagccagc cgcgagcgtg cgcaccaggt gcttgtgctc gggcagcgcg
      121 gccgccgcgc gggtgccagc gatgatctgg tcgcgcacct cgggggtgcg ggccaagctc
      181 gcgtggatca catagatcgt agcggggccg ctgggcccgt ttgcgcgata ggcgtctgcg
      241 atgcgcagag gccagagtgg ttgtccaagg acatagtcac cgagcgcgac gccagcttgt
      301 agcttggctt ggctcccgga ggtggtccca ggttgcgccg tcatcgacga ggagccccca
      361 tggcgtgacg gttaggatac gggccaggac ggagcagggt caatcggctg ggcagccagc
      421 ctgggtcgct agcttcttcg ccgtcggatt cctcccgagc tcacgtaagt acttgcaacc
      481 gcggtcgaga tcgcccgatg cgatcaggcc ctccgcgagt aggaatcgcg ccttcacgtt
      541 gcgcgaatcg agctggagcg cagcatccgc cttttgcacc cagcgcacca gcttgcgacc
      601 gcgcttcgcc tcttcggcct ccgcgatcag tttcgcgatc tcggggttgc ttcccttgcg
      661 ggtgatctgc acgcctgcgt ccaccggcac tgcggcatcg acggcgatcg cgggcgcatc
      721 ggcaacggca gcatccgcaa tctccgcgtc gaccggtttg tcggccgcct tggcgaccgg
      781 cgaaggcgtg gtgggcgatc gcttcttcat cgcgaccacg atcgcggcgg cgatcatgcc
      841 cgcgacgatc accgcgccga cgacgagcca catgccgcgc cgcttcggtt gcggcatttc
      901 gatcgcatcg gttccgctcg gcggcagcgt gccgatcttg atcgactgcc cgggcatcat
      961 catgccgtcg ccgggcttca tcgtcgcggc ggccgcgctg gccttgggtg atacttgctg
     1021 cggcaacagc gtcgcgttgg gatcgagcgc ggtgacgtag gtcgacggcg agaacaccgg
     1081 gccgcgcatc tcgggcagcg gtgcgcgcac tttgccgagc gagtgctcga ccgacgcgag
     1141 catctgcgcc atttgttcgg cggtctgcgg tcgtgcgtcg cgctgcttcg cgaggcagcg
     1201 ctgcagcacg gcatcgagct cggtgtactg cgcgagcgac ggtgcgccgt cggcgaggtg
     1261 cggcgctggc gtgcgcgcgt gatgcgtgag ccattcgatc gcggatttcg cgcgatacat
     1321 gccgcagccg gtgagcatcg cgaacatcgt cgccgcgagc gaatacagat cgctccggcc
     1381 atcgagttgc tgcccgcacg cttgctccgg cgacatgaac tcgggcgtgc cgaacaccat
     1441 gcccgcttgc gtgatcgcga gcatcgagcg atcggatcgc gcggcgccct ccatcagctt
     1501 ggcgacgccg aagtcgagca ctttcacgaa atcgggatcg tctcccgagc gcatgagcag
     1561 caagttgtcc ggcttgacgt cgcggtggat gagcccgctg cgatgtgccg caccgagcgc
     1621 cgcacatgct tgcttcgcga tgtggacgac gcgcccggga tcgaacggcc cgtcgacgtc
     1681 cagctcccgc tgcactgtac ggccatcgag gtactcgagg accatgtagt agtccttgtg
     1741 cggcgtcaca ccaaaatcga gcaccttgac cgcgtggtcc gactcgacgc gcatcgttgc
     1801 gcgggcctca cgggcgaacc gctggagcgc cgcctcgtcg gtagtcagat gtggcttgag
     1861 cagcttgacc gcgaccttgc gatccatcgc gaggtgatgt gcgcggaaca ccgcacccat
     1921 gccgccttcg ccgagcaacg catcgagtcg ataacggtcg tcgaggacga caccgaccag
     1981 tccggccgca aagctctcct cagccactgg cttagaaacg catgccgttc tttacccgga
     2041 acgagaacca gtcccaggcg aggtacgcgg cgcccgctgc agcgatcacc ccgaccacag
     2101 gtgcgcctgc gaccgcggcg acacccgcac cgatcgccgt ggcggcggca agcgtgccct
     2161 tgcgcttcgc tacgaccttg ttgcgctgga cgagggagct cacttctgga ggctgcaccg
     2221 agaattaccc cctgtcaaac cgccgccgcc gcgctcgcgc gcagccgtgg cggatctgtg
     2281 acacggggcg gcgtgtctcg tcggccacgc tggatgcgtg cgcacagaca cacggtgtgc
     2341 ggtggaacag agttgccctg gccccaagtc tgcaaactcc aggatctgcg cgggagcatg
     2401 ataagaagga gagagatgtc accgaacggt ctcggtcaac gcgacgcagt tctcttcggt
     2461 ctcgcaggcg gaatgcttgt cgtcggcgca gcgctcgccg gtggctatta cgtgatcagc
     2521 acgcgtagcg aagcgcgcgc ggcgacacgg ccgcccgttg tcgaagcgcg catcgatgca
     2581 gtcacgcact caccgggtgc atccggtggt gcggcgctcg acgcgttccc gtcgaaggtc
     2641 gtcgaggtga agaccggtgg cggcatcgcg aagaagacgt gggccgagct cggcatcgag
     2701 atcgatcgcg acgagctggg acgcgcgggc ttggtgaaca gcgagcagca gctcgccgcg
     2761 ctcgcgaaga agggctcgat cccggttcgc atcgatcgcg ataaggcggt caaagcgatc
     2821 atcgggctca agggccagta cgacaaagct gcgatcgacg cgcacctcga tctcgaagcg
     2881 cgcaagatcg agagcgactc gcccggtgag ggcatcgacg tctgggcatc gctgccgaag
     2941 atcgtgagcg ctgcgcgcag cggcgttacc tcgttcgagc tcgtatcggt cgcggtaccg
     3001 gcgcgcgtca cgaagcagac gctcggcatc gacgacatct cgaacgtgct cggccagtac
     3061 tcgacgaagt tcgcggtcac cgacaaggat cgtaacttca acctcaagct cgcggcgtcg
     3121 cacatcaacg gtgcggtgct gcaacccggc gtcgagtggt cgttcaacgg caccgtcggc
     3181 gaacgctcac aggcaaccgg ctacaagatc gcgcacgtga tcaccgcggg cgagatggtc
     3241 gacggcctcg caggcggtac gtgccagatc tcgacgacgc tatttggcgc gtcgttcttc
     3301 gccggcctcg acatcgtcaa gacgctcaac cactcgcgac caagcgcgta cacgccgctc
     3361 ggcttcgacg cgaccgtcgt gtggcccgac accgacctcg tgctgcgcaa cccgtacgac
     3421 ttcccggtcg cggtgctcta tcgcgtcgcg aacggcgaag cgcacgtcga gatcctcggc
     3481 aagaagcggc cgtggacgaa ggtgcagttc gtccgcgagg tcatcgaaga gaacccgtac
     3541 tccaccgagg agcgcatcga cgaagagatg ccgatgggcc tcacgatgac cgatcagcca
     3601 ggcttcaacg gctacaagct caagcgctac cgccgcttct tccagggcaa gaagatggtg
     3661 aaagagaaca agtggacggt gaactacaag ccggtcaccg aatacatccg tcgcggcagc
     3721 agcatggatc cgaacgtcgt ggcgccgaaa gagaaggagc aacacctgct caagccggtg
     3781 aagcccgaag agatgaacac cgagaaggtg atcctgacgc agtaggcgct acgacccgta
     3841 cgggtcgtcg atgtctttcg ggcttgccgt cggcgcttgt ggcatctgag cgacgccgca
     3901 gttcatgttc actttcgcgc ccgacatcgt cacgccgccg ctgcccttga tcgtcgcgtt
     3961 cgcgccggtg ccccactgca tcgcctgtcc ggcgtgaatc tcgacgttgc cggtcgcttt
     4021 gaattcggcg gtcttcgcga cgaccttcat gtcgccggtc gggcactgga agcagacgtc
     4081 accttcgatt gcctcgatga ggatcttgtt cgcgttcgaa tcgaacgtga tctttccttt
     4141 gccggtgccg tcttcgatga tgatcttcat ctgatcgtcg tcgaaggtga tcttggagcc
     4201 ctggcgcgac ttgatcacgc ggcgcggatt gccgccgggg ttcttgtcgg ccggcttgtc
     4261 cttgccgttc cacagcgcgc cgacgatgac cggacgatcg agatcgccgt gctcgaacat
     4321 gcagagcacc tcgtcatcga cttcgggaat gaagaaccag ccgcggttct taccggcgcc
     4381 gagcatgatg atcggacacc accacgacgt gtccttgtcc gagaggatcg ggaacttcac
     4441 cttcacgcga cccagcttct gcgggtcctt gttgtcggtg acgactccga tcaccggctc
     4501 gtagcacttg ttggcttctt gctcggccgt ccggatctgc gccgcaaaca cgcttgcgag
     4561 gtcgtaaagc gggcgcatgg tctcgatact actacggttg atttggggcg ttgcttcccg
     4621 ccggaggcgt ggccgaagtc gccagtggga ggacaccgag gacgatgaga ttcgggtcgt
     4681 tagccggtgc cgccccggtc gcgagcgact tcgcgccggc cggctcactg gcgaccaggc
     4741 cgacggcgag gccgttcccg agccaatccg aggggatcgt gacggtgaac ttgtcccgga
     4801 tgtactcgcc cgtccgccag cggtcgctgg ggaagaagcc gtcgccggtc ggccgcatcg
     4861 gcgagcgttg catcgtggtc ggtgcgggat cggtcggctt ccaggccggc gtcgtgaccg
     4921 gccagaccgc gagcaggaag cgatacgcga tcggcgtccg ctctttgacc ttgaagtaga
     4981 cgtcgaggtc gacgcgcgag ccgatcgcgg gacgcgggct cttgtccgcg acgccgacgc
     5041 cgaggatttc gagcttgccg tcgtcgagcg tctggccttg ggtgacgacc tcgggcgtcg
     5101 gcgcggccgt cagtacgagg tcggccatct gctttcctgc ctgattgaac ttggtgtcgc
     5161 gcgcatacag cacgcgctcg agccaggcat cgagcatcgt cttcatcgtc gtcatcgccg
     5221 gcggattctt cggcgcgagg ttcgtcttct ccaccgggtc ggccttgagg tcgtagagct
     5281 cgtacaggtt cgactgcacg tagaagatga gcttccaggc ttccgagatc gccgcgcgct
     5341 gcggcgacgg cgcgttcgtc tcggagaaca cgatgcggtc atgatcttcc ttgccataga
     5401 agatctgcgg cacgaggctg cgcccttcga acgaaaggtc cttggtgtcg atgccgagga
     5461 tgtcggtgat cgtcgggatg atgtcgagcg acgtcacggc gccaccgatc gtgcgcggct
     5521 gattgttcgg gatgtaaatg agcagcggcg tatgcaggag ctcgaaatag agcgcggtgc
     5581 cgtgggtgcc gaccggcacg gtgtgctcgc ccatgctgtc gccgtgatcg ctcgtgatga
     5641 tgatgatcgt cctgtcgttc gagggtaggc gcacgagctc gtcgaacagc ctcccgatct
     5701 gttgatcggt ccagcgcagc tcggcgtcgt agagatcggg ctcggacgcg ccgtagtcga
     5761 tgacgtcggg atgcgcgacg tagcggccgt gcggatcgat gtagtgcgcc cacatgaacc
     5821 acttgtgacc ttgctggcgc gagatccacg cgagcgtgtg gtcggtgacg cggtcggcgg
     5881 cgacgatgaa c
//
LOCUS       DASUFX010000812         6303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1104440, whole genome shotgun sequence.
ACCESSION   DASUFX010000812 DASUFX010000000
VERSION     DASUFX010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104440"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFV99_32870"
     CDS             <1..449
                     /locus_tag="VFV99_32870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064229.1"
                     /translation="AIDHLAEMRARELKMLASQLPDGRARTDAVLELLWQCFAGTFFQ
                     VSMELRTAARTDPELRPVLITAERALRDRILAQAKSLFGKDVSEHPGLERALDFTLQF
                     MIGAAMTAVLHKEDGGRLRELMDDFKNLFPTVLTNATTKKRTKVTR"
     gene            446..3433
                     /locus_tag="VFV99_32875"
     CDS             446..3433
                     /locus_tag="VFV99_32875"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5064230.1"
                     /translation="MSKELRFDGKVAIVTGAGGGLGRSHALLLASRGAKVVVNDLGGS
                     FTGEGKSGTAADKVVAEIKEKGGEAVANYDSVEDGDKIVKTAIDAFGKIDILINNAGI
                     LRDVSFQKMTQADWDLVYKIHVFGTYKVTAAAWNLMRDAGYGRIINTSSAAGIYGNFG
                     QANYSMAKLGIHGFTQTLAAEGRKRNVIVNTIAPIAGSRMTETVLPKELLDALKPEYV
                     SALVAKLAHESMEETGGLYEVGGGFYSKLRWERTKGKVFRLGRTITPDDVDASWKEIT
                     SFDKADHPGSVAESMQPIMGNVEAGPSKGGNQFIDVDAALGFTYPEQTWTYDTRDVSL
                     YALGVGAAKDPTNEADLALVYEMSGKGMKVLPSFGVLPAINMVFDQAKRGITAPGLNF
                     GLDRVLHGEQYTEVVRPLPTTGKLSTKAKVKDIFDKGKNALVVTEFTTYDENGDVLIK
                     NELTTFVRGAGGWGGDRGPSADINVPPDRAPDKVIEDKTSENQALLYRLSGDWNPLHA
                     DPSFAKAFGFQRPILHGLCSFGVATRAAATMAPDGDPRFIKSIKARFASTVLPGETLV
                     TELWKEGNKVIFRTKIKERGEVCISNAAVELWTELPKPKPKAAPAQAGASAAAVPTSA
                     DIFRAIGGFVAGNPATAEKVKTNFLFKLSNPDAQWTIDLTTPPGSVSEGAVGKAVCTL
                     DISDADFMAMATGQADAMKLFSSGKLKISGDVMASQKLGFLKKITPEMVLAETKKRTG
                     AGSGAAAPSAAGGLPADYQPTTEDAFDVIQEFLNQNPSVTEKVGVVYGWKVGGKSYLL
                     DLKSVPGKVTQGEGAAECTLELAEEDFLAMTQGKADPMKLFTSGKLKISGNIMASQKL
                     NFLAKMDPSKAIEVIAKKKGIGGGGAAASAPAAAAAPAASKPAGEPNAPKFFAALTKR
                     LAENAGLKNEVKATVTFNVTDPNTSQKFDLGSGAQATLTIADADLPALASGNPRSLYQ
                     HGKLRVDGDASVAQRLGFLKGLL"
     gene            3448..4629
                     /locus_tag="VFV99_32880"
     CDS             3448..4629
                     /locus_tag="VFV99_32880"
                     /inference="COORDINATES: protein motif:HMM:NF006102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX5064231.1"
                     /translation="MPRKVNVIGVGMVPFQKPGKSEEYHVMAQKAGTAALADAKIPYN
                     EVQQAYAGYVYGDSTCGQRAIYQLGLTGIPVFNVNNNCSTGSSALMLGTQAVAGGMAE
                     CVIVVGFEQMEAGALQAKWTDRANPLDKFVNVMNEEQGFNSAPPAAQMFGGAGREYRW
                     KHGTKRETFGKIAEKARLHASKNPYALFKETYSLEQIMQSPEVFDPLTRYQCCPPTCG
                     AAAAVLCSDDFAKKHNISKPVYIAGQVMTTDFPSSFEEHSMIKMVGFDMAKNAAKKLY
                     EMTGVGPEDVDVVELHDCFTANELLTYEAIGLCKEGEAEDFIWKGDNTYGGKFVTNPS
                     GGLLSKGHPLGATGLAQCTELVWHLRGTAEARQVEGAKIALQHNLGLGGACVMTMYRR
                     D"
     gene            4646..5545
                     /locus_tag="VFV99_32885"
     CDS             4646..5545
                     /locus_tag="VFV99_32885"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5064232.1"
                     /translation="MRCLLCLALLLIGVIGCREQNTEPPSGHEIQPPRSAAKLAPEAG
                     LAAPQANLMGTVADGVVTAPSGGVTQKLFADDVASDELALTIGIETIGDVATPLFPRG
                     TKLPTTHTEVFSTALDDQNRVEVHVLQGERPLASQNRSLGKFQLFGIPPAPRGVPQIE
                     VTFAIDKDGVLTVSARDKATSQSKQIIIDGAAGALDKAAVDKMLAEAAAAKADDDIRR
                     SWSQAKNDLDQMIYSSKKLMQDVGSKLSKKTRTRVEQEIKGAEGVLAVSTKPADPKLL
                     LAVTDSLRKAMHSAAEELYKTKP"
     gene            complement(5715..>6303)
                     /locus_tag="VFV99_32890"
     CDS             complement(5715..>6303)
                     /locus_tag="VFV99_32890"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064233.1"
                     /translation="YPDEDWTWKTVDEYLLGDRILVAPILTEGATSRPVKLPGGAWTR
                     LSGGDPLVGDIEASASPSEIPVYVKSGALLVLYPGTVDTMLAAPTLASATTLAETNDE
                     REIIVYAGNPGNPAHGQWNDETGPEGTPQYTWSGRPMGTLPASATFNGTPVSVTKLAS
                     EARVTITGDGTLELEGGGTLTIARGVTATYSVIVR"
BASE COUNT         1233 a         2042 c         2080 g          948 t
ORIGIN      
        1 ccgcgatcga ccatctggcc gagatgcgcg cccgcgagct caagatgctc gcgtcccagc
       61 tccccgacgg tcgcgcccgc accgacgcgg tactcgagct gctctggcag tgcttcgccg
      121 gcacgttctt ccaggtgtcg atggagctgc gcaccgcggc gcgtaccgat ccggagttgc
      181 ggccggtgct catcaccgcc gagcgcgcgc tgcgcgatcg cattctggcg caggcgaaga
      241 gcctgttcgg caaagacgtg agcgaacatc ccggcctcga gcgggcgctc gacttcacgc
      301 tgcaattcat gatcggcgcg gcgatgactg ccgtccttca caaggaagac ggcggccggc
      361 tccgcgagtt gatggacgac ttcaagaatc tgtttccgac ggtgttgacc aacgcaacaa
      421 ccaagaagcg cacgaaggtg acccgatgag caaagagctg aggttcgacg gaaaggtagc
      481 aatcgtaacc ggcgcaggcg gtggcttagg ccgctcgcac gcgctcctgc tcgcgtcgcg
      541 aggcgcgaag gtcgtcgtga acgacctcgg cggcagcttc accggcgaag gcaagtccgg
      601 cacagcggcc gacaaggtcg tcgccgagat caaagagaag ggtggcgaag cagtcgccaa
      661 ctacgactcg gtcgaggacg gcgacaagat cgtcaagacg gcgatcgatg cgttcggcaa
      721 gatcgacatc ctcatcaaca acgccggcat cctccgcgac gtctcgttcc agaagatgac
      781 gcaggcggac tgggacctcg tctacaagat ccacgtcttc ggcacctaca aggtgaccgc
      841 cgcggcgtgg aacctcatgc gcgatgcggg ctacggccgc atcatcaaca cgtcgagcgc
      901 cgccggcatc tacggcaact tcggccaggc gaactactcg atggccaagc tcggcatcca
      961 cggcttcacg cagacgctcg ccgccgaagg ccgcaagcgc aacgtcatcg tcaacacgat
     1021 cgcgccgatc gccggctcgc gtatgaccga gaccgtgctc ccgaaggagc tgctcgacgc
     1081 gctcaagccc gagtacgtct cggcgctcgt cgcgaagctc gcacacgagt cgatggaaga
     1141 gaccggcggc ctctacgagg tcggcggcgg cttctactcg aagctgcgct gggagcgcac
     1201 gaagggcaaa gtgttccggc tcggtcgcac gatcacgccg gatgacgtcg atgcgtcgtg
     1261 gaaagagatc acgagcttcg acaaggcgga tcatcccggc tcggtcgccg agtcgatgca
     1321 gccgatcatg ggcaacgtcg aggccggccc gagcaagggc ggcaatcagt tcatcgacgt
     1381 cgacgccgcg ctcggcttca cgtatcccga gcagacgtgg acgtacgaca cgcgcgatgt
     1441 gtcgctctac gcgctcggcg tcggcgccgc gaaggatccg acgaacgaag ctgacctcgc
     1501 acttgtctac gagatgagcg gcaagggcat gaaggtgctg ccgagcttcg gcgtgctgcc
     1561 cgcgatcaac atggtgttcg atcaggcgaa gcgcggcatc acggcgccgg gcctgaactt
     1621 cggcctcgac cgcgtgctcc acggcgagca gtacaccgag gtcgttcgcc cgctgccgac
     1681 gaccggcaag ctgtcgacga aggcgaaggt caaagacatc ttcgacaagg gcaagaacgc
     1741 gctcgtcgtc accgagttca cgacgtacga cgagaacggc gacgtgctca tcaagaacga
     1801 gctcaccacg ttcgtgcgtg gcgccggtgg ctggggcggc gatcgcggcc ccagcgccga
     1861 catcaacgtg ccgcccgatc gcgcgcccga caaagtgatc gaggacaaga cgagcgagaa
     1921 ccaggcgctg ctctatcgct tgagcggcga ctggaacccg ctgcacgccg acccgagctt
     1981 cgcgaaggcg ttcggcttcc agcgtccgat cctgcacggc ctgtgctcgt tcggcgttgc
     2041 gacccgcgcc gccgcgacga tggcgcccga cggtgacccg cggttcatca agagcatcaa
     2101 ggcgcgcttc gcgtcgacgg tgctccccgg cgagacgctc gtgaccgagc tgtggaaaga
     2161 gggcaacaag gtcatcttcc gcaccaagat caaagagcgc ggcgaggtgt gcatctcgaa
     2221 cgcagcggtc gagctgtgga ccgagctgcc gaagcccaag ccgaaggcgg cgccggcgca
     2281 agcgggtgct tctgcggcgg cggtaccgac cagcgcggac atcttccgcg cgatcggtgg
     2341 cttcgttgcg ggcaaccccg cgacggccga gaaggtaaag acgaacttcc tgttcaagct
     2401 ctccaacccc gatgcgcagt ggacgatcga tctgacgacg ccgccgggct cggtgagcga
     2461 aggcgccgtc ggcaaggcgg tgtgcacgct cgacatcagc gatgcggact tcatggcgat
     2521 ggcgacgggc caggccgatg cgatgaagct gttctcgtcg ggcaagctga agatcagcgg
     2581 cgacgtgatg gcgtcgcaga agctcggctt cctcaagaag atcacgccgg agatggtgct
     2641 cgcggagacg aagaagcgca cgggcgctgg cagcggcgca gcggcgccga gtgcagcggg
     2701 cggtctgccg gcggactacc agccgacgac cgaggacgcg ttcgacgtga tccaggagtt
     2761 cctgaaccag aacccgagcg tcaccgagaa ggtcggcgtc gtgtacggct ggaaggtcgg
     2821 cggcaagagc tacttgctcg acctcaagag tgtccccggc aaggtgacgc aaggtgaggg
     2881 cgctgccgag tgcacgctcg agctggcaga agaggatttc ctcgcgatga cgcagggcaa
     2941 ggccgatccg atgaagctgt tcacgagcgg caagctcaag atcagcggca acatcatggc
     3001 gtcgcagaag ctgaacttcc tcgccaagat ggatccgagc aaggcgatcg aagtgatcgc
     3061 gaagaagaag ggcattggcg gtggtggcgc agcagcgtcg gcaccggccg cagcggctgc
     3121 accggcggcg agcaagccgg ccggcgagcc gaacgcgccg aagttcttcg cggcgctcac
     3181 caagcgtctc gccgagaacg ccggtctcaa gaacgaagtg aaggcgaccg tgacgttcaa
     3241 cgtcaccgac ccgaacacct cgcagaagtt cgacctcggt agtggcgcac aggcgacgct
     3301 gacgatcgcc gacgcggatc tacctgccct cgcgagcggc aacccgcgct cgctctacca
     3361 acacggaaag ctacgcgtcg acggagatgc ttctgtcgcg caacgtctcg gcttcctcaa
     3421 aggcctgctc taaggagaga aatgaccatg ccccgcaaag taaatgtcat cggcgtcgga
     3481 atggttccgt tccagaagcc gggtaagagc gaggagtatc acgtgatggc gcagaaggcc
     3541 ggtacggccg cgctcgccga cgcgaagatc ccgtacaacg aagtgcagca agcgtacgcc
     3601 ggctacgtgt acggcgattc gacctgcggc cagcgcgcga tctaccagct cggcctcacc
     3661 ggcatcccgg tgttcaacgt caataacaac tgctcgacgg gcagctcggc gctcatgctc
     3721 ggcacccaag cggttgctgg cggaatggcc gagtgcgtga tcgtcgtcgg cttcgagcaa
     3781 atggaagccg gcgcgctcca ggcgaagtgg accgatcgcg cgaacccgct cgacaagttc
     3841 gtcaacgtga tgaacgaaga gcagggcttc aactcggccc cgcccgccgc gcagatgttc
     3901 ggtggcgcag gtcgcgagta ccgttggaag cacggcacga agcgcgagac gttcggcaag
     3961 atcgccgaga aggcgcgcct ccacgcgtcg aagaatccgt acgcgctgtt caaggagacg
     4021 tactcgctcg agcagatcat gcagtcgccc gaggtgttcg atccgctgac gcgctaccag
     4081 tgctgcccgc cgacgtgtgg cgcggccgcg gcagtgctgt gctcggatga cttcgcgaag
     4141 aagcacaaca tctcgaagcc ggtgtacatc gccggccagg tgatgacgac ggacttcccg
     4201 tcgtcgttcg aagagcactc gatgatcaag atggtcggct tcgacatggc gaagaacgcc
     4261 gcgaagaagc tgtacgagat gaccggtgtc ggtcccgagg acgtcgacgt cgtcgagctg
     4321 cacgattgct tcaccgcgaa cgagctgctc acgtacgaag cgatcggcct gtgcaaggaa
     4381 ggcgaagccg aggacttcat ctggaagggt gacaacacct acggcggcaa gttcgtcacg
     4441 aacccgtcgg gcggcctctt gtcgaagggt cacccgctcg gcgcgaccgg tctcgcgcag
     4501 tgcaccgagc tcgtctggca cctccggggc accgccgagg ctcgccaggt cgagggcgcg
     4561 aagatcgcgc tgcaacacaa cctcggtctg ggtggcgcct gcgtcatgac gatgtatcgt
     4621 cgcgactgat gtatcgtcgc gactgatgcg ctgcctgctc tgcctcgcgc ttctcctgat
     4681 cggcgtgatc ggttgtcgcg agcagaacac cgaaccgccg agcggccacg aaatccagcc
     4741 gccgcggagc gctgcaaagc tcgcccccga ggcgggcctt gcggcgccgc aggcgaatct
     4801 gatgggcacc gtcgccgacg gcgtggttac cgcgccgagc ggcggcgtca cccagaagct
     4861 gttcgccgac gacgtcgcat cggacgagct ggcgctgacg atcggcatcg agacgattgg
     4921 cgacgtcgcg acgccgctgt ttccgcgcgg cacgaagcta ccgacgacgc acaccgaggt
     4981 gttctcgacc gcgctcgacg atcagaaccg cgtcgaagtt cacgtgcttc agggcgagcg
     5041 gccgctcgcg agccagaacc gctcgctcgg caagttccag ctgttcggga ttccgccggc
     5101 gccccggggc gtgccgcaga tcgaggtcac gttcgcgatc gacaaggacg gcgtgctcac
     5161 ggtcagcgcg cgcgacaagg cgaccagcca gagcaagcag atcatcatcg acggagcggc
     5221 cggcgcgctc gacaaggcag cagtcgacaa gatgctcgcc gaggcagccg ccgcgaaggc
     5281 cgacgatgac atccggcgca gctggagcca ggcgaagaat gatctcgatc agatgatcta
     5341 ttcgagcaag aagctcatgc aggacgtcgg cagcaagctg tcgaagaaga cgcgaacgcg
     5401 cgtcgagcag gagatcaaag gcgccgaggg cgtgctcgcg gtcagcacga agccggccga
     5461 tccgaagctg ctcctcgcgg tgaccgactc gctgcgtaag gcgatgcact ccgccgccga
     5521 agagctctac aagacgaagc cctagccacc ggacaccgca cgccggacac cgcgcccgga
     5581 caccgcggcg tttccacgat gcgtgccgtc ctgcggagat gcgttagcat ttcgtcgtcc
     5641 aggcaacgct cccatacgaa catcatcttc gtctgagagg gcccttagcg gcccttagcg
     5701 gcccttagcg gcccttagcg gacgatcacg ctgtacgtcg cagtgacgcc gcgcgcgatc
     5761 gtgagcgtgc cgccaccttc gagctcgagc gtgccgtcgc cggtgatcgt cacgcgcgcc
     5821 tcgcttgcca gcttcgtcac gctcactggc gtgccattga acgtcgccga cgccggcagt
     5881 gtgcccatcg gccggccgct ccacgtgtac tgcggcgtcc cctcgggccc agtctcgtca
     5941 ttccactgcc cgtgcgcagg gtttcctggg ttccccgcgt acacgatgat ctctcgctcg
     6001 tcgttcgttt ccgcgagcgt ggtcgccgac gcgagtgtgg gcgccgcgag catcgtgtcg
     6061 acggtgcccg gatagaggac gagcagcgcg cccgacttca cgtacaccgg aatctcggaa
     6121 ggcgatgcac tggcctcgat gtcaccgacg agcggatcgc caccggaaag ccgcgtccag
     6181 gcgccacccg gcaacttcac aggacgcgag gtcgcgcctt cggtgaggat cggcgccacg
     6241 aggatccgat cgccgagcag atactcgtcg acggtcttcc atgtccagtc ctcgtcggga
     6301 tac
//
LOCUS       DASUFX010000813         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1520020, whole genome shotgun sequence.
ACCESSION   DASUFX010000813 DASUFX010000000
VERSION     DASUFX010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520020"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFV99_32895"
     CDS             <1..849
                     /locus_tag="VFV99_32895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5064234.1"
                     /translation="LSTERRPTHPPIESLPLVQLARTPLSQRVVDWVGTYIDQVRLLG
                     KRTADLHCALASDPTNLLFAPEPYDIMHQQSMYGSCSAYMARTFEQLRGHLDSLMPET
                     QSLAEAVLAREGDIDRVLEHITRRRIDCIRTRIHGDYHLGQVLWTGDDFIIIDFEGEP
                     GRPLSQRRFKRNPLRDAAGMLRSLEYASEAALRDGRNRPEDLPVLQEWAHAWSQWASA
                     AFLAGYFENVAGKRILPPNDADLELLLRFFLLEKVIYEISYELNNRPDWAEIPMRGLL
                     ALLQMP"
     gene            846..>2683
                     /gene="glgB"
                     /locus_tag="VFV99_32900"
     CDS             846..>2683
                     /gene="glgB"
                     /locus_tag="VFV99_32900"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX5064235.1"
                     /translation="MKDPSSPGDAPRGVPEFGELDAHLMREGKHPRLHEKLGSHPVAG
                     GTYFATWAPHAAAVSVIGDFNNWRPGANALEPIGDTGVWHGVVPNVGVGALYKLHIRS
                     KLRSYEVAKADPFALRHEVPPGTASIVWTPEHTWRDDAWMAARAARSACEAPVSIYEV
                     HLGSWMRVPEEGNRSLTYREAAPKLVDYVTKLGFTHVELMPLTEHPFYGSWGYETTGY
                     FAATSRYGAPEDLMAMIDALHAANIAVILDWVPAHFPTDEHGLVYFDGTPLYEHPDRR
                     LGFHPEWNTSIFDFGRQEVRSFLLSSALFWLERFHVDGLRVDGVASMLYRDYGRKPGE
                     WIANDKGGNEYFEAVELLQRLNDAIAREQPSAITVAEESTAWPKVTAKPSEGGLGFSY
                     KWDMGWMHDTLRYLAHDPIHRRHHHNELTFRGMYAWSERFVMPLSHDEVVYGKGSLLG
                     KMPGDRWQKFANLKLLYAYLWSQPGKKLLFMGGEFAQWSEWNHDTSLDWHLLDDISHR
                     QIQLLVGELNRLYRTEPALHELDCEPAGFRWLSVDDAERSVLVYERISRDDQRMICAL
                     NFTPVPRPNYRVGVPSAGLWSEILNTDAQELGGSGHGNLGSIEAAPV"
BASE COUNT          480 a          898 c          852 g          453 t
ORIGIN      
        1 ttatcgacgg agcgtcggcc gactcacccg ccgatcgaat cgctaccgct cgtgcagctc
       61 gcacgcacgc cgctcagcca gcgcgtggtc gactgggtcg gcacgtacat cgaccaggtg
      121 cggctgctcg gcaagcgaac cgcagacttg cactgcgcgc tcgcgagcga cccgacaaac
      181 ctgctgttcg ctccggagcc gtacgacatc atgcatcagc aatcgatgta cggttcgtgc
      241 agcgcgtaca tggcgcgcac gttcgagcag ctgcgcggcc atctcgactc gctgatgccc
      301 gagacgcaga gccttgccga ggcagtgctc gcgcgggaag gcgacatcga ccgcgtgctc
      361 gagcacatca cgcgccgccg catcgactgt atccgcacgc gcatccacgg cgactatcac
      421 ctcggtcagg tcctgtggac cggtgacgac ttcatcatca tcgacttcga gggcgaaccc
      481 ggtcggccgc tgtcgcaacg ccgcttcaag cgcaatccgc tgcgcgatgc cgccggcatg
      541 ctgcgctcgc tcgagtatgc gagtgaggcc gcactgcgcg acggcagaaa ccgccccgag
      601 gacctgccgg tgctgcagga atgggcacac gcgtggtcgc agtgggcatc cgcggcgttc
      661 ctcgctggct acttcgagaa tgtcgccggc aagcgcatcc tgccgccgaa tgacgccgac
      721 ctcgagctgc tgctgcgctt cttcttgctc gagaaggtga tctacgagat cagctacgag
      781 ctgaacaacc gccccgattg ggccgagatt ccaatgcgcg gcttgctcgc gctgctccag
      841 atgccatgaa ggacccctcc tcgcccggtg acgcaccgcg cggcgtgccc gagtttggcg
      901 agctcgacgc gcacctgatg cgcgagggca agcatcctcg cttgcacgag aagctcggct
      961 cgcatccggt cgccggcggc acgtacttcg cgacgtgggc gccgcacgcg gcagcggtca
     1021 gcgtgatcgg cgacttcaac aactggcggc caggcgcaaa cgcgctcgaa ccgatcggcg
     1081 ataccggcgt gtggcacggc gttgtaccga acgtcggtgt cggcgcgctc tacaagctgc
     1141 acatccgcag caagctgcgc agctacgagg tcgcgaaggc tgatccgttc gcgctgcgcc
     1201 acgaggtgcc acccggcacg gcatcgatcg tgtggacacc cgagcacacg tggcgcgacg
     1261 acgcatggat ggctgcgcgc gccgcacgat ccgcgtgtga ggcgccggtg tcgatctacg
     1321 aggtccacct ggggtcgtgg atgcgcgtcc ccgaggaggg caaccgttcg ctcacgtatc
     1381 gcgaagcggc gccgaagctc gtcgactacg tgacgaagct cggtttcaca cacgtcgagc
     1441 tgatgccgct gaccgagcat ccgttctacg gctcgtgggg gtacgaaacg accggctact
     1501 tcgctgcgac gtcgcgctac ggcgcacccg aagatctgat ggcgatgatc gatgcgctgc
     1561 acgccgcgaa catcgccgtg atcctcgact gggttccggc gcactttccc accgacgagc
     1621 acggcctggt ctacttcgac ggcacgccac tctacgagca ccccgatcgc cgcctcggtt
     1681 ttcacccgga atggaatacg tcgatcttcg acttcggccg ccaggaagtg cgcagcttcc
     1741 tgttgtcgag tgcattgttc tggctcgagc gctttcacgt cgacggtcta cgtgtcgacg
     1801 gcgtcgcgtc gatgctctat cgcgactacg gccgcaagcc cggcgagtgg atcgcgaacg
     1861 acaaaggcgg caacgaatac ttcgaagccg tcgagctact ccagcgcctc aacgacgcga
     1921 tcgcgcgcga acaaccgtcg gcgatcacgg tcgccgagga atcgaccgcg tggccgaagg
     1981 tcaccgcgaa gccgagcgag ggcgggctcg ggttttcgta caagtgggac atgggatgga
     2041 tgcacgacac gctgcgctac ctcgcgcacg atccgatcca tcgccggcat caccacaacg
     2101 agctcacgtt ccgcggcatg tacgcgtgga gcgagcgctt cgtgatgccg ctctctcacg
     2161 acgaggtcgt ctacggcaaa ggctcgctgc tcggcaagat gcccggcgat cgctggcaga
     2221 agttcgcgaa tctgaagttg ctctacgcgt acctgtggtc gcagccgggc aagaagctgt
     2281 tgttcatggg cggcgaattc gcgcagtggt cggaatggaa ccacgacacg agcctcgact
     2341 ggcatctgct cgacgacatc tcgcatcgcc agatccagct gctcgtcggc gagctcaacc
     2401 gcctctatcg gaccgagccc gcgcttcacg agctcgactg cgagcccgct ggatttcgct
     2461 ggctcagcgt cgacgatgcc gagcgcagcg tgctcgtcta cgaacgaatt tcgagagacg
     2521 accagcgcat gatctgtgcg ctgaacttca cgccggtgcc gcgccccaac taccgcgtgg
     2581 gcgttccgag cgcgggtttg tggagcgaaa tcctcaatac cgacgcgcag gagctcggcg
     2641 gtagcggtca cggcaacctc ggtagtatcg aagcagcgcc ggt
//
LOCUS       DASUFX010000814         7126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1799953, whole genome shotgun sequence.
ACCESSION   DASUFX010000814 DASUFX010000000
VERSION     DASUFX010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799953"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /gene="hypF"
                     /locus_tag="VFV99_32905"
     CDS             complement(<1..1959)
                     /gene="hypF"
                     /locus_tag="VFV99_32905"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX5064236.1"
                     /translation="MRHQARPSCAHRLRLRSDTFGATHSHTRAPAREMVPCVRGSTSA
                     LGGPLYAAGERVDERRSIRVRGVVQGVGFRPAMYRLAESLGLAGFVRNDREGVWIEIE
                     GPGKMLERFAAEVRGAAPPASRIDEIEQAVVPVRRERGFAIAESPRDAESTHASAEIP
                     VDLGPCDACLRELEDPTNRRYRYPFINCTNCGPRFTIVREVPYDRPKTTMAAFAMCAP
                     CLCEYESPSDRRFHAEPNACPACGPRLQLVIDRRVMLEGDDALRAAASAIADGRIVAV
                     KGAGGFVLAVDACSERAVVRLREHKRRPHKAFAVMVGSLAHAEIVAELDDRAREVLQS
                     PGRPIVLVPACRGHLAAAVAPGLTDVGIFLPATPLQHLLLADGPSVQVMTSGNLVDEP
                     IARTNEEAFTRLIDVADMFLVHDREVHARTDDSVVRSSRRGMIPMRRARGLVPNAIAL
                     PSAGPPVLAVGAHERNTVCLAHGGKAVVSQHIGDLDHPDSEAFFRETIAHLEQLAGVR
                     AEVVVHDLHPDYQSTRWAMACGLRRIAVQHHHAHVASCLAEHGRSDRVIAVAFDGTGL
                     GDDGTLWGGEILCADLTSSQRLGHLRPIALAGGEAAIRAPWRLAAAALVDAREPLDVV
                     PADAADRERVRALLATNLPTRVTGCGRWF"
     gene            1979..2308
                     /locus_tag="VFV99_32910"
     CDS             1979..2308
                     /locus_tag="VFV99_32910"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX5064237.1"
                     /translation="MHELALMESVVDAVVDQIGEERVAVVRLEIGELAGVAIDALKFS
                     FDVCTQGTVLAGAELAIVQVTGMARCRSCGREQATSSFATPCTCGSFDRELVRGGELR
                     LIDVEVY"
     gene            2308..3081
                     /gene="hypB"
                     /locus_tag="VFV99_32915"
     CDS             2308..3081
                     /gene="hypB"
                     /locus_tag="VFV99_32915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX5064238.1"
                     /translation="MCRTCGCDGVDHDHEDDHGHDHDHGHEHDHGHDHHHHHHRPRRI
                     ALEADLLAKNDRLAGALRDRFAHDHIVALNLIGAPGAGKTTLLEHTLRAVPELSIAVI
                     EGDQATDHDARRIAATGRRAIQVNTGAGCHLEADMIERGLGALGLVPQSLLFVENVGN
                     LVCPALFDLGEQAKVVVVSVTEGDDKPLKYPHIYRAASAMVVTKMDLAPHIDADPAQL
                     IANARKVNPGLAAFPVAATRGSGMAEWCGWLRAQMGAQR"
     gene            3078..3620
                     /locus_tag="VFV99_32920"
     CDS             3078..3620
                     /locus_tag="VFV99_32920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064239.1"
                     /translation="MIDDDRLRAESVEIEYMLEEIKELVAEPAWQRIERVIARVVRLY
                     GAGLAHTIEHARSAGVTSATFDESMAADDLLASLLALHGLHPLAVDVRVERALATARA
                     ELGLAADDLVLVAVHDRHVELRATADLGGGAMASRVAEAAIRRVIESVAPEITAIDIH
                     GIAPVRDPSLVQLRVRREAP"
     gene            3617..4231
                     /locus_tag="VFV99_32925"
     CDS             3617..4231
                     /locus_tag="VFV99_32925"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEX5064240.1"
                     /translation="MIAFRSLARLAATDAITRADVRCELCGGPLTDTHRHVVELGQRG
                     VQCVCHACGILFARGSSARFRTIPDRVRIASLFDLTPERWAELGIPVALAFCYRDSLR
                     GCGVVCYPGPAGVTDAALEPAVWDAICDATPLAADLEADVEALIVRGGRGATTLRCYL
                     VPISTAYELVGRLRRSWQGFSGGEEAEAELASFFAELERRGERP"
     gene            4228..5391
                     /locus_tag="VFV99_32930"
     CDS             4228..5391
                     /locus_tag="VFV99_32930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064241.1"
                     /translation="MNFETARVLADAVLMEGYALYPYRASSLKNRFRWTFGVLAPRAW
                     AKSSANEPWWLQVQVLVAGPVERIAGRLRFFQIERRHADEGSTITHWDEGVLREVDFV
                     IEDGRTCVPFAFDGGWSRTGTEARERRAIVGDIVLERERVSAEDLLTKLSIRVENHTP
                     WSDQDAPRDRVMIAALASTHLLLGVDSGHLLSALDPPAWARNAAICTNVRAYPILVGP
                     EGSGDVVLAGPFIMQDYPQIAPESAGDLCDATEIDELLMLRTRTMTDAEKREARATDA
                     RAAAIIDRADGLPAEWLARMHGAARSLSQGEMIPKLPPLAVGSKVRLRAPTTRTDAQD
                     LLYAGQVATVVELREDVDGTTFLGVTVDADPAAELHRWYGRCHYYRVDEVEPL"
     gene            5388..5849
                     /locus_tag="VFV99_32935"
     CDS             5388..5849
                     /locus_tag="VFV99_32935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX5064242.1"
                     /translation="MKRVLVAGVGNVFFGDDGFGVEVARQLAGPMPTGTRVLELGIRA
                     VHFAFELLEPIDLLIVADCMARGGAPGTLYVVEPQAEAPPPALADAHGMNLSIVFATL
                     QQLGGTLPPTRIVGCEPATIEPGIGLSTVVTHAIPGAIELIHDLISRQEAS"
     gene            complement(5972..6349)
                     /locus_tag="VFV99_32940"
     CDS             complement(5972..6349)
                     /locus_tag="VFV99_32940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064243.1"
                     /translation="MKPALAVVLLLSGAAYADNRPDNGPPPPSRPHGQLRQLLLQRFD
                     RNHDGRLEPRERRQAAKALRRLANKMMEGNPRQARARKFIRRFDLDRDGNVGPNEIPP
                     GLADDLRPLDRDGDGWLRGDELP"
     gene            complement(6388..>7126)
                     /locus_tag="VFV99_32945"
     CDS             complement(6388..>7126)
                     /locus_tag="VFV99_32945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064244.1"
                     /translation="PEERLVLGRLSLSQVPSAEPRTQDAKAAQLADIFGLGSVAIEMA
                     GGTPPLGDETPARLTDLRPDLPSEISDLVDWLLVPEPIARPQSAQDVLSQLDAVIERM
                     GSSSRSLRILIVDDDAARARWFYGLARRAHPATVVETAGEGTDAAHKLNRDQPDLVFI
                     NAGLRGVMNAFELCMYARGLASEHHTVGRLVLIGSVSERDLALFRDSNVEAIPDDAQL
                     PSRILDRIRAVSAAKPRQRQTRTTISG"
BASE COUNT         1143 a         2349 c         2452 g         1182 t
ORIGIN      
        1 gaaccagcga ccacaaccgg tcacgcgggt cggcaggttt gtcgcgagta gcgcgcgcac
       61 gcgttcgcgg tcggccgcgt cggcgggaac cacgtcgagg ggttcgcgag cgtcgacgag
      121 cgcagcagcg gcgaggcgcc acggcgcccg aatcgcggct tcaccgcccg cgagcgcgat
      181 cggtcgcagg tgaccgagtc gttgcgacga cgtcaggtcc gcgcacagga tctcgccgcc
      241 ccacagcgtg ccgtcgtcgc caaggcccgt accgtcgaat gcgacggcga tcacgcgatc
      301 gctgcggccg tgctccgcca ggcacgaggc gacgtgggcg tgatgatgtt gcacggcaat
      361 ccggcggaga ccacacgcca tcgcccaccg cgtcgactgg tagtcgggat gcaggtcgtg
      421 gacgacgact tcggcgcgca cgccagcgag ctgctcgaga tgcgcgatcg tctcgcgaaa
      481 gaacgcctcc gagtctgggt gatcgaggtc gccgatgtgc tgcgatacga cagccttgcc
      541 gccatgcgcg aggcacaccg tgttgcgctc gtgcgcgccg accgccagca ccggcgggcc
      601 agcgctcggc agcgcgatcg cgttcggcac gagaccgcgc gcacgccgca tcggaatcat
      661 gcctcgtcgc gagctgcgca ccacggagtc gtcggtacgt gcatggacct ctcgatcgtg
      721 gacaaggaac atgtcggcaa catcgatgag ccgggtgaac gcctcttcgt tcgtgcgagc
      781 gatcggttcg tcgacgagat tgccgctcgt catcacctgg accgagggcc catcggcgag
      841 tagcagatgc tgtagaggtg tcgccggcag gaagatgccg acgtcggtga ggccgggcgc
      901 gacggcagcc gcgagatgac cacgacatgc cgggacgagc acgatcggcc gtccaggcga
      961 ttgcaacact tctcgcgcgc ggtcgtcgag ctccgccacg atctctgcgt gcgcgagaga
     1021 tccgaccatc actgcgaagg ccttgtgcgg gcgacgcttg tgctcgcgta gccgaaccac
     1081 cgcgcgctcg gagcaggcat cgaccgcaag cacgaagcca cctgcacctt tgaccgcgac
     1141 gatgcggcca tcggcgatcg cactcgctgc ggcgcgcaag gcgtcgtcgc cttcgagcat
     1201 gacgcggcga tcgatcacga gctgcaatcg cggcccacag gcagggcagg cgttcggctc
     1261 cgcgtggaag cggcggtcgc tcggcgattc gtattcgcaa aggcagggcg cgcacatcgc
     1321 aaacgccgcc atcgtcgtct tcggtcggtc gtacgggacc tcgcgtacga tcgtgaagcg
     1381 cggcccgcaa ttggtgcagt tgatgaacgg gtatcggtac cggcggttcg tcggatcctc
     1441 gagctcgcgc aaacacgcgt cgcacgggcc gaggtcgaca ggaatctccg cgctcgcgtg
     1501 cgtagactct gcatcacgcg gactctcggc aatcgcgaag ccgcgctcac ggcgcacggg
     1561 gaccacggcc tgttcgatct cgtcgatgcg cgacgcaggt ggtgccgcgc ccctgacctc
     1621 cgcggcgaag cgttcgagca ttttgcccgg cccctcgatc tcgatccaaa cgccctcgcg
     1681 gtcgttccgg acaaacccag cgaggccgag cgactccgcg aggcgataca tcgccggcct
     1741 gaagccgacg ccttgcacca cgcctcggac tcggatcgat cgccgctcgt ccactcgctc
     1801 gccggctgca tatagaggac caccgagcgc gctcgtgctc ccgcgtacgc acggcaccat
     1861 ttcgcgcgct ggcgcacgcg tgtgcgagtg agtcgcgccg aacgtgtcac tgcgtagccg
     1921 aagtctgtgc gcgcacgacg gccgcgcttg gtgtcgcatt cgcaacgagg cgcgcgacgt
     1981 gcacgagctg gcgctgatgg agagtgtggt cgatgcggtc gtcgatcaga tcggcgaaga
     2041 gcgcgtcgcg gtcgttcgtc tcgaaatcgg cgagctagca ggcgtcgcga tcgatgcgct
     2101 gaagttcagc ttcgacgtct gtacgcaagg caccgtactc gcaggtgctg agctggcgat
     2161 cgtgcaagtt acgggcatgg cccgttgtcg gagctgcggg cgcgagcaag cgacgtcgtc
     2221 attcgcgacg ccgtgtacat gcggcagctt cgaccgcgag ctcgtgcgcg gcggcgagct
     2281 tcgactcatc gacgtggagg tctactaatg tgccgaacct gcgggtgcga tggcgtcgac
     2341 cacgatcatg aggatgacca cggccatgac catgaccacg gccatgagca tgaccacggc
     2401 catgaccacc accaccatca tcaccgaccg cgtcgcatcg cgctcgaggc cgacctgctc
     2461 gcgaagaacg atcggctcgc cggggcgctg cgcgatcgct ttgcgcacga tcacatcgtc
     2521 gcgctgaatc tgatcggcgc acccggcgcg ggcaagacga cgctgctcga gcacacgctg
     2581 cgtgcggttc ccgagctgtc gatcgcggtg atcgagggtg atcaagcgac cgaccacgat
     2641 gcgcgccgga tcgccgcgac cggtcgccgc gccatccagg tcaataccgg cgccggctgc
     2701 catctcgagg ccgacatgat cgagcgcggt ctcggagcgc tcggcctcgt gccgcagtcc
     2761 ctgttgttcg tcgagaacgt cggcaacctg gtgtgtcccg cgctgttcga tctcggcgag
     2821 caggccaagg tggtcgtcgt gtcggtcacc gaaggcgatg acaagccgct caagtacccg
     2881 cacatctatc gtgccgcgag cgcgatggtc gtcaccaaga tggatctggc gccgcacatc
     2941 gacgccgatc cggctcagct gatcgcgaac gcgcgaaagg tcaatcccgg gctggcagca
     3001 tttccggttg cagcgacgcg cggctcgggc atggcggagt ggtgcggctg gctgcgggcg
     3061 cagatggggg cgcagcgatg atcgacgatg atcgtctgcg tgccgagtcc gtcgagatcg
     3121 agtacatgct cgaagagatc aaggagctcg tcgccgagcc ggcctggcag cgcatcgaac
     3181 gcgtgatcgc gcgtgtcgtt cgtttgtatg gcgctggtct cgcgcacacg atcgagcacg
     3241 cacgcagtgc cggcgtcaca tcggcgacgt tcgacgagag catggcggcc gacgatctcc
     3301 tcgcgagcct gctcgcgctc catggacttc atccactcgc ggtcgacgtt cgcgtggagc
     3361 gtgcgctggc caccgcgcgt gccgagctcg gtctcgcggc cgatgatctc gtgctcgtgg
     3421 ccgtgcacga tcgacacgtc gagttgcgcg cgactgcgga tctcggtggt ggagcgatgg
     3481 cgtcgcgcgt ggcggaagcc gcgattcgcc gcgtgatcga aagcgtcgca cccgagatca
     3541 cggcgatcga catccacggg atagcaccgg tgcgcgatcc aagcttggtc cagctccgcg
     3601 tccgtcgtga ggcaccatga tcgcgttccg ctcactcgca cgcctcgccg ccaccgacgc
     3661 gatcacgcgc gccgatgtcc ggtgcgagct gtgtggtggt ccgctcaccg acacgcatcg
     3721 ccatgttgtc gagctcggcc agcgtggtgt gcagtgcgtt tgtcacgcgt gcggcatcct
     3781 gttcgcacgc ggcagcagtg cacggtttcg cacgattccc gaccgcgttc gcatcgcgtc
     3841 gctattcgat ctcacgccgg agcggtgggc cgagctcggg atcccggtcg cgctcgcgtt
     3901 ctgctatcgc gattcgctgc gcggctgtgg cgtcgtttgt tatccagggc cggcgggcgt
     3961 caccgatgct gcactcgagc ccgcggtgtg ggatgcgatc tgcgacgcga ccccgctcgc
     4021 tgcggatctc gaagccgatg tcgaagcgct gatcgtgcgc ggcggccgcg gcgctacgac
     4081 gctccggtgt tatctggttc cgatcagcac ggcgtacgag ctcgtcggtc gcttgcgccg
     4141 aagctggcag ggcttctcgg gcggcgagga ggctgaagcg gagctcgcca gcttcttcgc
     4201 cgagctcgag cgccgaggag aacggccatg aacttcgaga ctgcgcgcgt actcgcggac
     4261 gcggtgctga tggagggcta cgcgctctat ccgtatcgcg cgtcgtcgct caagaatcgc
     4321 ttccggtgga cgttcggcgt gctcgcgccg cgcgcgtggg caaagagcag cgccaacgaa
     4381 ccctggtggc tccaggtgca agtcctggtc gctggcccgg tcgagcgcat cgctggccgc
     4441 ttgcgattct tccagatcga gcggcggcac gccgacgagg gaagcacgat cacgcactgg
     4501 gacgaaggcg tgttgcgcga ggtggacttc gtcatcgaag acgggcgcac gtgtgtgccg
     4561 ttcgcgttcg atggtggctg gtcgcgcacg ggcaccgaag ctcgcgagcg tcgcgcgatc
     4621 gtcggtgaca tcgtgctcga gcgcgagcgc gtttcggcgg aagacctgct gacgaagctg
     4681 tcgatccgcg tcgagaacca cacgccgtgg agcgatcaag atgcaccgcg cgatcgggtg
     4741 atgatcgccg cgctcgcatc gacgcatctg ctcctcggtg tcgatagtgg ccatctgctg
     4801 tcggcgctcg atccacccgc gtgggcacgc aacgctgcca tctgcacgaa cgtgcgcgcg
     4861 tacccgattc tcgtcggacc cgaaggcagc ggcgacgtcg tgctcgcggg gccgttcatc
     4921 atgcaggact atcctcagat cgcaccggag agcgccggcg atctctgcga cgcgaccgag
     4981 atcgacgagc tgctgatgct gcgcactcgc acgatgacgg acgcggagaa gcgcgaggca
     5041 cgtgctactg atgcgcgcgc ggctgccatc atcgatcgtg cagacgggct gcccgcggaa
     5101 tggctcgcgc gcatgcatgg tgccgcgcgc tcgctgtcgc agggcgagat gattccgaag
     5161 cttccgccgc tcgcagtcgg cagcaaggtc cggctccgcg caccgacgac acgaaccgat
     5221 gcgcaggact tactctacgc gggtcaggtc gccaccgtcg tcgagctccg cgaggacgtc
     5281 gacggcacga cgtttctcgg cgtcacggtc gacgccgatc cggcggccga gctgcatcgc
     5341 tggtacggcc gctgtcacta ctatcgcgtc gacgaggtcg agccgctgtg aaacgagtgc
     5401 tcgtggcagg cgtcggcaac gtgttctttg gcgacgatgg cttcggcgtc gaggtcgcgc
     5461 gtcagctcgc agggccgatg ccgacgggaa cgcgagtgct cgagctcggc attcgcgcgg
     5521 tgcacttcgc gttcgagctg ctcgagccga tcgatctatt gatcgtggcg gactgcatgg
     5581 ctcgcggagg cgcgccgggg acgctctacg tcgtcgaacc ccaagccgag gctccgccgc
     5641 cggcgctggc cgatgcgcac ggcatgaacc tgtcgatcgt gttcgcgacg ttgcaacaac
     5701 tcggcggcac gctgccaccg acgcgaatcg tcggctgcga gcccgcgacc atcgagcccg
     5761 gaatcgggct gagcactgtc gtcacgcacg cgattcctgg cgcgatcgag ctgattcacg
     5821 acctcatctc acgacaggag gcctcatgac gaacacgaaa cgcaacgcac tactgatcat
     5881 cgccggcgca atatcgatcg cagcattcgt cttgcgcggc gcggtgcccg agctcgtccg
     5941 ctacatgcgg atccggcgga tgtagcccgc gttacggcag ctcgtcgccg cgcagccagc
     6001 cgtcgccatc gcgatcgagt gggcgcaggt cgtcggcgag gccaggtggt atttcgttcg
     6061 ggccgacgtt gccgtcgcga tcgagatcga agcggcgaat gaacttgcgc gcgcgtgcct
     6121 ggcgcggatt gccttccatc atcttgttcg cgagccgccg cagtgccttc gcagcttgcc
     6181 ggcgttcgcg cggctcgagt cggccatcgt gattccgatc gaagcgctgg agcagcagct
     6241 ggcgcagctg accgtgggga cgtgagggcg gcggtggtcc attgtccggt cggttgtccg
     6301 cataggcagc gccggagaga agcagaacga cggccagggc gggcttcata cccacccaga
     6361 ctcgtggcag ggcaggacgt taaatgccta ccccgagatg gtcgtgcggg tctggcgctg
     6421 tcgtggtttc gccgccgaga ccgcgcggat ccggtcgagg atccggctcg ggagctgggc
     6481 gtcgtcgggg atggcctcga cgttggaatc ccggaacagc gcgaggtcgc gctccgagac
     6541 gctgccgatc agcaccagac ggccgaccgt gtggtgctca ctcgcgagac cacgcgcgta
     6601 catgcagagc tcgaacgcgt tcatgacgcc gcgcaaccct gcgttgatga acaccaggtc
     6661 gggctggtcg cggttgagct tgtgcgctgc gtcagtacct tcgccggcgg tctcgacgac
     6721 cgtcgccggg tgtgcgcgcc gtgcgagccc atagaaccag cgtgcacgcg ccgcgtcgtc
     6781 atcgacgatc aagatccgca acgaccgcga gctgctgccc atccgctcga tcactgcatc
     6841 gagctgtgac agcacgtcct gcgcgctctg tggccgcgcg atcggttcgg gcacgagcag
     6901 ccaatcgacg agatccgaga tctcgctcgg cagatccggg cgcaggtcgg tgagacgcgc
     6961 gggcgtttca tcgccgagcg gcggcgtgcc gccggccatc tcgatggcga ccgagccgag
     7021 gccaaagatg tcggcgagct gcgctgcttt cgcgtcctgc gtgcgcggtt cggccgacgg
     7081 cacctgcgac agcgacaagc ggccgagcac cagacgctct tccggc
//
LOCUS       DASUFX010000815         5418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_687830,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000815 DASUFX010000000
VERSION     DASUFX010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687830"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VFV99_32950"
     CDS             <1..1301
                     /locus_tag="VFV99_32950"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5064245.1"
                     /translation="KKRGFTVVTYGNGADALAALSDHDVDVVVTDLNMKGMTGLEVCQ
                     RIVADRPDIPVILLTAFGSFETAVGAIRAGAYDFVTKPVEIEALAIAIRRAGEHRQLR
                     GEVKRLREAVESTRGRGDLIGASPAMRQVYDLIDQVSTTDATILITGESGTGKEVVAR
                     AVHDRSRRKDGPFVAINCAAMPEALLESELFGHAKGAFTDAKANRLGLFQQANGGTLF
                     LDEIGEMAIALQPKLLRAIQERKVRPVGAESEVPVDVRIIAATNRDLEEMVEDKRFRE
                     DLYYRINVIHIPLPPLRARGGDVLMLAQHMLRQYAVVHDKKVVGLSPAAAERLVAYEW
                     PGNVRELGNCLERAVALAHFEEIQVDDLPDKIRLRSRAPSLSGTDLPELLTLEEVERR
                     HVLRVLEACNGNRTDAAKMLGLDRKTLYRKLLRWGVGDES"
     gene            1298..1810
                     /locus_tag="VFV99_32955"
     CDS             1298..1810
                     /locus_tag="VFV99_32955"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5064246.1"
                     /translation="MTRFAVREAALEDAEGIARVHTDSWQTSYRGILPDTVLDRIDVG
                     QRAESRRKILRDRSVFQLVAYDVTHGDIVGFCDAGASRRHVPYAGEVYAIYLAHRAKR
                     HGIGQEMWERVQAWLVANDMRSMIVWVLENNHHARAFYEAMGGRTGSRLQSRVGGYPV
                     VELSYVWERL"
     gene            1970..2395
                     /locus_tag="VFV99_32960"
     CDS             1970..2395
                     /locus_tag="VFV99_32960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064247.1"
                     /translation="MRNLIVALTLALFAGPALADTPPAAKPADTKTAAPAKDAKAAPA
                     KDAKAAPAKDAKAAPAKDAKATDTKAADAKPATDAKASDTKAADTKASDTKATDTKTT
                     TKAPAKKPAAKKAAAPKAPASDAKAAEPKPATDAKAATK"
     gene            complement(2397..4052)
                     /locus_tag="VFV99_32965"
     CDS             complement(2397..4052)
                     /locus_tag="VFV99_32965"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5064248.1"
                     /translation="MSETPTANHDFDYVVVGSGFGGAVSACRLAEKGYAVAVLEMGKR
                     WTEADYPKSTWNLRRWIWRPGLKLFGFYNMRPFRHVMILCGNAVGGGSITYANTLLVP
                     PEIVWREGSWKGLAEWEREMPAHYATAKRMLGVTENKILGDADHMLKKMADDQGVGET
                     FYRTDVAVYFGDGPAKVHADPFFGGEGPARAGCIGCGGCMVGCRYNAKNTLDKNYLYF
                     AEKRGAKIIAETRVVDIKPLGAADGSDGYTITTERSTAWFAKARTTITAKHVVLSASA
                     LGTMDLLMRMKRSGSLPAISDKLGDDVRTNSESIVGVRFPGTDLDMSKGIAIGSGIHI
                     DRFTHIEATRYPRGSDVLGLLGTLLVKGTGTGRIFKWMGAAFRHPIKFARAAWPFGFA
                     RQTLIFLVMQTIDATLRFRLRRRWWWPFQEHLVSEGTRIPTNIPQANDFTEKAAAKFG
                     GIPITSSSEILFNMPMTAHCIGGCVMGEDATQGVIDAQHRVFGYRNLLVVDGSAVGAN
                     LGVNPSLTITALAERAMSFVPAKTELPAHRPLPMAGSSVRIET"
     gene            4103..5296
                     /locus_tag="VFV99_32970"
     CDS             4103..5296
                     /locus_tag="VFV99_32970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064249.1"
                     /translation="MGRTSRKAWVMASGAVGLAAALTAAALVAPNRAAAAAQTYVPHD
                     AATIVAHVPPRDPREVAERQALAAAPGRVELAVAIARGEIEKARALSDPRYLGRAQAT
                     LAHWWKLAQPPADVLLLRATIEQSLHQFVAARTDLDQLIARRPDDVQAHLTRAVVATV
                     RGDYAAARESCAALTQLASPLIALTCQAPLDALAGNVDDAYRRLAAALNGRVQPELRT
                     WALTTLAELAIQSGDDSRAAAHLRAVLAIDPDDVYARAALADIVAPDEASALLAGYEA
                     IDTLLVRRAIAEDRVNGPDAAKLVREMRARFASAAEREDRVHLREEAMFVLAVDNDPD
                     RALDLARKNWDVQKELADARLLAQAAVEAKQPRAAAPVIAWAHATGVRDARLAHWLDR
                     LGDMQ"
BASE COUNT          950 a         1813 c         1811 g          844 t
ORIGIN      
        1 tcaagaagcg cggcttcacg gtcgtcacgt acggcaacgg cgccgatgcg ctcgcagcgc
       61 tctctgatca cgacgtcgat gtcgtcgtca ccgatctcaa catgaaaggc atgaccggcc
      121 tggaggtgtg tcagcgcatc gtcgccgacc gtccggacat cccggtcatc ctgctcaccg
      181 cgttcggcag cttcgagaca gccgttggtg cgatccgcgc tggcgcgtac gacttcgtga
      241 cgaagcccgt cgagatcgaa gcgcttgcga tcgcgatccg tcgcgccggc gagcatcgcc
      301 agctgcgcgg cgaagtgaag cgcctgcgtg aagcggtcga gtcgacgcgc gggcgcggcg
      361 atctgatcgg cgcgagcccg gcgatgcgtc aggtgtatga cctgatcgat caggtctcga
      421 ccaccgacgc gacgatcctg atcacgggcg aaagcggcac tggcaaagaa gtcgtcgcgc
      481 gtgcggttca cgatcgcagc cggcgcaagg atggcccgtt cgtcgcgatc aactgcgcgg
      541 cgatgccgga ggcgctgctc gagagcgagc tcttcgggca tgcgaagggt gcgttcaccg
      601 acgcgaaggc gaatcgcctc ggtctgttcc agcaagcgaa tggtggcacg ctgttcctcg
      661 acgagatcgg cgagatggcg atcgcattgc agccgaagtt gttgcgcgcg attcaagagc
      721 gcaaggtacg gccggtcggc gccgagtccg aagttccggt cgacgtccgc atcatcgcgg
      781 cgacgaatcg cgacctcgaa gagatggtcg aggacaagcg cttccgcgaa gacctctact
      841 accgcatcaa cgtcatccac atcccgctac cgccgctgcg cgcgcgtggc ggcgacgtgc
      901 tgatgctcgc gcagcacatg ttgcgccagt acgcggtggt ccacgacaag aaggtcgtcg
      961 gcctgtcgcc ggctgcggcg gagcggctcg tcgcgtacga gtggcccggc aacgtgcgcg
     1021 agctcggcaa ctgcctcgaa cgcgcggtcg cgctcgcgca cttcgaggag atccaggtcg
     1081 acgatctacc cgacaagatc cgtcttcgct cgcgcgcacc atcgctgtcg ggcactgact
     1141 tgcccgagct gctcacgctc gaagaagtcg aacgccgaca cgtgctgcgc gtgctcgaag
     1201 cctgcaacgg caaccgcacc gatgccgcga agatgctcgg actcgatcgc aagacgctct
     1261 accggaagct gctacgctgg ggagtcggag acgaatcatg acgcggtttg cagtgcgcga
     1321 agcagccctc gaagacgccg aaggcatcgc gcgcgtgcac acggacagct ggcagacgag
     1381 ctatcgcggc attcttcccg acaccgtgct cgatcggatc gacgtcggcc agcgcgccga
     1441 gtcgcgccgc aagatcctgc gcgatcgatc cgtgttccag ctcgtcgcat acgacgtcac
     1501 gcatggcgac atcgtgggct tctgcgatgc gggcgcgagc aggcgccacg ttccgtatgc
     1561 aggcgaggtc tacgcgatct atctcgcgca tcgcgcgaag cgacacggca tcggccagga
     1621 gatgtgggaa cgcgtacagg cgtggctcgt ggcgaacgac atgcgatcga tgatcgtgtg
     1681 ggtgctcgag aacaatcacc acgcgcgcgc gttctacgaa gcgatgggtg gacgcaccgg
     1741 ttcgcgcctg caatctcggg tcggcgggta cccggttgtc gagctctcgt acgtctggga
     1801 acgtctctaa acgacaggcg ttttgcggcg acaactcgag cttcgaggcg aattgccccg
     1861 cgggggcaag ctgccccgag atgcgcactg caactcgcgg aattcgtgga ccgcgcgagg
     1921 cacatcggct gcacaacacg cggcgctcat caaaggagaa ttcaagctca tgcgtaatct
     1981 gatcgtcgct ctgaccctcg ccctcttcgc tggtcctgcg ctcgccgaca cgccgccggc
     2041 cgccaagccg gctgacacca agaccgcagc gcccgcgaag gacgcgaagg cagcgcccgc
     2101 gaaggatgcc aaggcggcac ccgcgaagga cgcgaaggcg gcgcccgcga aggacgcgaa
     2161 ggcgactgac accaaggcag ccgacgccaa gccagcgacc gacgcgaagg cgtcggacac
     2221 caaggcggcc gacacgaagg cgtcggacac caaggcgacc gacaccaaga cgaccacgaa
     2281 ggctccggcg aagaagccgg ccgcgaagaa ggctgcagcg ccgaaggcgc cggcgagcga
     2341 cgcgaaggcg gcggagccga agccggcgac cgacgcgaag gccgcgacga agtaacttac
     2401 gtttcgattc gcacggagct gcccgccatc ggtaatggcc gatgtgcggg cagttctgtt
     2461 ttggcgggca cgaacgacat cgcacgttcg gcgagcgccg tgatcgtgag gctcggattg
     2521 acgccgaggt tcgcgccaac cgcggagccg tcgacgacga gcaggttgcg atagccgaac
     2581 acgcggtgct gcgcgtcgat cacgccctgc gtggcgtcct cgcccatcac gcagccgccg
     2641 atgcagtgcg cggtcatcgg catgttgaac aggatctccg acgacgacgt gatcggtatg
     2701 ccgccgaact tcgccgcggc cttctcggtg aagtcgttcg cctgcgggat gttcgtcggg
     2761 atgcgcgtgc cctcggagac caggtgctcc tggaacggcc accaccagcg ccggcgcagc
     2821 cggaagcgca atgtcgcgtc gatcgtctgc atgacgagga agatgagcgt ctggcgcgcg
     2881 aagccgaacg gccacgccgc gcgtgcaaac ttgatcggat gacggaacgc ggcacccatc
     2941 catttgaaga tgcgccctgt gccggtgcct ttgacgagca acgtgccgag taggccgagc
     3001 acgtcggaac cgcgcgggta gcgcgtcgcc tcgatgtgcg tgaaccgatc gatgtgaatg
     3061 cccgagccga tcgcgatgcc cttcgacatg tcgagatcgg tcccgggaaa gcgcacgcca
     3121 acgatcgact cggagttcgt gcgcacgtcg tcgccgagct tgtcgctgat cgcgggcagc
     3181 gagccactcc gcttcatccg catcaataga tccatcgtgc cgagcgcaga cgccgacagc
     3241 acgacgtgct tcgcggtgat cgtcgtgcgc gctttcgcga accacgccgt cgagcgctcg
     3301 gtcgtgatcg tgtagccgtc cgagccatcg gcggcgccga gcggtttgat atcgaccacg
     3361 cgcgtctcgg cgatgatctt cgcgccgcgc ttctcggcga agtagaggta gttcttgtcg
     3421 agtgtgttct tcgcgttgta gcggcagccg accatgcagc cgccgcaacc gatgcagccg
     3481 gcgcgcgccg gtccttcgcc gccaaagaac ggatcggcat gcaccttcgc ggggccgtcg
     3541 ccgaagtaga ccgcaacgtc ggtgcgatag aacgtctcgc ccacgccctg atcgtcggcc
     3601 atcttcttca gcatgtgatc ggcgtcgccg aggatcttgt tctcggtcac gccgagcatg
     3661 cgcttcgccg tcgcgtagtg tgccggcatc tcgcgctccc actccgcgag gcctttccac
     3721 gatccttcgc gccacacgat ctcgggcggg acgagcaacg tgttggcgta cgtgatcgag
     3781 ccgccgccga ccgcgttgcc acacaagatc atcacgtggc ggaacggccg catgttgtag
     3841 aagccgaaca gcttgagccc gggccgccag atccagcgcc gcaggttcca cgtcgatttc
     3901 ggatagtcgg cttccgtcca ccgcttgccc atctcgagca ccgcgacggc gtaacccttc
     3961 tcggcgagcc ggcacgcgga aacggcgccg ccgaaacccg agccgacgac gacatagtcg
     4021 aagtcgtgat tcgcggtagg ggtttcggac atcgtgcgca ctatacgatc cggttgtcgc
     4081 tcacgctcgt agttgtgggg cgatgggaag gacgagccgc aaggcgtggg tgatggcgtc
     4141 gggtgccgtc ggattggcgg ctgcgctgac cgctgccgca ctggtggcac cgaaccgggc
     4201 tgccgccgca gcgcagacgt acgtgcctca cgacgcggca acgatcgtgg ctcacgtacc
     4261 accgcgtgat ccgcgcgaag tcgcggaacg ccaagcgctt gcggcggcac ccggccgcgt
     4321 cgagctcgcg gttgcgatcg cacgcggcga gatcgaaaag gcccgcgcgc tgagtgaccc
     4381 gcgttacctg ggccgcgctc aggcgacgct tgctcactgg tggaagcttg ctcagccgcc
     4441 ggccgatgtg ctgctgctgc gtgcgacgat cgagcagtcg ctacatcagt tcgtcgccgc
     4501 gcgcaccgat ctcgatcagc tgatcgcgcg tcgccccgat gacgtgcagg cccacctcac
     4561 gcgcgcggtc gtcgccacgg tgcgcggtga ctacgccgct gcgcgcgaga gctgcgccgc
     4621 gttgacgcag ctcgcatcgc cactgatcgc actcacgtgt caggcgccgc tcgacgcact
     4681 cgcgggcaat gtcgacgacg cgtatcgccg gctcgcggcc gcgctgaacg gacgcgtgca
     4741 gcccgagctg cgcacgtggg cgctgaccac gcttgccgag ctcgcgatcc agagcggcga
     4801 cgactcgcgg gcggccgcgc atctgcgcgc agtgctcgcg atcgatcccg acgatgtcta
     4861 cgcccgcgct gcgcttgccg acatcgtggc gcccgacgag gcctccgcgc tgctcgccgg
     4921 ctacgaggca atcgatacgc tgctcgtgcg gcgcgcgatc gcggaggacc gcgtgaacgg
     4981 tcccgatgct gcgaagctcg tccgcgagat gcgcgcgcgg ttcgcctctg ccgccgaacg
     5041 cgaggatcgc gtgcaccttc gcgaggaagc gatgttcgtg ctcgccgtcg ataacgatcc
     5101 ggaccgtgca ctcgatctcg cgcgcaagaa ctgggacgtc cagaaggagc tcgccgatgc
     5161 gcgcctcctc gcacaagccg ctgtcgaagc aaagcagcca cgtgctgcgg cgccggtgat
     5221 cgcatgggca cacgcgacgg gcgttcgcga cgcgcgcctc gctcactggc tcgaccgcct
     5281 cggagacatg caatgaggca agcccatgac gttggcttcg cgcgcgcgtg ccacgtgctc
     5341 gcacgcaagg ccgccgagac acacctgcgg ctcgccaccc acggcgtcgc gggcgccgcg
     5401 caattcgaag tcgccgcg
//
LOCUS       DASUFX010000816         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1800332, whole genome shotgun sequence.
ACCESSION   DASUFX010000816 DASUFX010000000
VERSION     DASUFX010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800332"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VFV99_32975"
     CDS             complement(<1..1519)
                     /locus_tag="VFV99_32975"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5064250.1"
                     /translation="MRMRLLAPLFALAACSGAGRVPTTSRVEAEPHRDSVSLEPAAPG
                     LRLPDGVTPLSYDVTLDVDPASENFFGEAHVRVRLDRASDHIWIHAHELAIKSARYDG
                     GELTTLPIKGDQMIGFGFGKTLPPGEHELVFVYSGKTTGDQEGLFRQKRGEHWYLFSQ
                     GESVFARRITPCFDEPRFKTPWRVTLVVPRAAAALANAPEENTTVEGTKKRVRFAETP
                     VMASYLLTIAVGPFDVVDVGTVGKQKVPVRVAVRAGAGKQVSVVASKLPAIVDAMEAY
                     VGEPLPLAKLDLVGVPQFFGAMENPGIITFEDNMLVGKETKQRIEYFTGVAAHEIAHQ
                     WFGNSVTPAWWDDLWLSESFASFLGDKITAQLGGFADRPLFRALERREALDADAGPDA
                     IPLRHPIESNADPDDAFDAIAYQKGEAVLATFEAMVGEATFRDALREYLKSHRDRSAT
                     TFDFVASLTRATKPELGKAFEQYATLAGVPVVDLALRCDKKPALIASALDKRLVPV"
     gene            complement(1567..>2778)
                     /locus_tag="VFV99_32980"
     CDS             complement(1567..>2778)
                     /locus_tag="VFV99_32980"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5064251.1"
                     /translation="DPQKRAAMGDAAVALGRAVGYVGAGTVEFIADPQGNFYFLEVNT
                     RLQVEHPVTELTTGLDLVREQIRIARGERLGYAAAPPQQGWAIEVRLCAEDPERDYLP
                     TTGTLHAVDVPSTIRADIGVVAGSEIGIHYDSMLGKLIAHAPTRREAAQVLRRALDET
                     WVPGVVTNREHLAKILAHPGFLAGELDTHFLERHAGELAARPPGLDRIRVAAIAATLA
                     GIGKRGSPDALTLPGWRNVPFADQQVHYKLSDTDVLLGYHPEGGALVFSIGGKTTTVS
                     RVRFDPDDHVAFVEHGGHRRRVRVTTSGDRIYVLSEGIIYTFTEQPRFPDHAHQAVAG
                     GLVAPMPGKVVKVLVAEGQEVAAGSALVVLEAMKMEHTVRAADAGVVRALHVTVGDQV
                     DTDHLLAVVTA"
BASE COUNT          448 a          943 c          924 g          463 t
ORIGIN      
        1 ataccggcac gagccgcttg tcgagggcgc tcgcgatcag cgccggcttc ttgtcgcacc
       61 gcagcgccag gtcgacgacc ggcacgccag cgagcgtcgc gtactgctcg aatgccttgc
      121 cgagctcggg cttggtcgca cgcgtgagcg acgcgacgaa atcgaacgtc gtcgcgctgc
      181 gatcgcggtg cgacttcaag tattcgcgga gcgcgtcgcg aaacgtcgct tcgccgacca
      241 tcgcttcgaa cgtcgcgagc accgcctcgc ccttctggta cgcgatcgcg tcgaacgcgt
      301 cgtcgggatc ggcgttcgac tcgatcgggt gtcgcaatgg aatcgcatcg ggaccggcgt
      361 cagcatcgag tgcttcgcgc cgctcgagcg cgcggaacag cggccgatcg gcaaagccgc
      421 cgagctgcgc ggtgatcttg tcgccgagaa agctcgcgaa tgattccgat agccacaagt
      481 cgtcccacca cgccggcgtc accgagttgc cgaaccactg atgcgcgatc tcgtgcgcgg
      541 cgacgcctgt gaagtactcg atgcgctgct tcgtctcctt gccgacgagc atgttgtctt
      601 cgaatgtgat gatgccgggg ttctccatcg cgccgaagaa ctgcggcacg ccgacgagat
      661 cgagcttcgc gagcggcagc ggctcgccaa cgtacgcctc catcgcatcg acgatcgcgg
      721 gcagcttgct cgcgacgacg ctcacttgct tgccggcacc ggcgcgcacc gcgacgcgta
      781 cgggcacctt ctgcttgccg acggtcccca cgtcgacgac gtcgaacggg ccaaccgcga
      841 tcgtgagcag atagctcgcc atcaccggtg tctcggcgaa gcgcacgcgc ttcttcgtgc
      901 cctcgacggt cgtgttctcc tcgggcgcgt tcgcgagcgc cgctgctgca cgcggcacga
      961 cgagcgtcac ccgccacggc gtcttgaacc gtggctcgtc gaagcacggc gtgatccggc
     1021 gcgcgaacac cgactcgccc tgcgagaaca aataccagtg ctcgccgcgc ttctggcgaa
     1081 acaggccttc ctgatcaccg gttgtcttcc cgctgtagac gaagacgagc tcgtgctcgc
     1141 cgggcggcaa cgtcttgccg aagccaaagc cgatcatttg atcgcctttg atcggcaacg
     1201 tcgtcagctc gccgccgtcg tagcgcgcgc tcttgatcgc gagctcgtgt gcgtggatcc
     1261 agatgtgatc gctggcgcga tcgagtcgca cgcgaacgtg cgcctcgccg aagaagttct
     1321 cgctggccgg atcgacgtcg agcgtgacgt cgtacgaaag cggcgtcacg ccatcgggaa
     1381 gtcgcaggcc cggtgcggct ggctcgagcg acaccgagtc acgatgcggc tcggcctcga
     1441 cgcggctcgt cgtcggtacg cggccggcgc ccgagcaggc ggcaagcgcg aacagcggcg
     1501 cgagcaggcg catgcgcatc cacgatgcgt aacacacttg gtttgcgctg ctcggctcag
     1561 atcaaatcaa gcagtgacaa ccgcaagcag gtggtcggta tctacctgat cgccgaccgt
     1621 tacatgcagc gcgcgcacca cgccggcatc ggccgcgcgc acggtgtgct ccatcttcat
     1681 cgcctcgagc acgacgagcg ccgagcctgc ggcgacctcc tgcccttccg cgacgagcac
     1741 cttgacgacc ttgcccggca tcggcgcgac gagcccgccg gcgacggcct ggtgcgcgtg
     1801 gtcggggaac cgtggctgct cggtaaacgt gtagatgatg ccttccgaca gcacgtagat
     1861 gcgatcgcca ctcgtcgtga cgcgcacgcg gcggcggtgg ccaccgtgct cgacgaacgc
     1921 cacgtgatcg tcaggatcga agcgcacgcg cgataccgtc gtggtcttgc cgccgatcga
     1981 gaacaccagc gcgccgcctt cggggtggta gccgagcagc acgtcggtgt cggagagctt
     2041 gtaatggact tgttggtcag cgaacggcac gttgcgccag ccgggtagcg tcaacgcatc
     2101 gggtgacccg cgcttgccga tgccggcgag cgtcgcggcg atcgcggcga cccggatgcg
     2161 atcgagaccg ggcggccgcg cggcgagctc gcctgcatgc cgctcgagga aatgcgtatc
     2221 gagctcgccg gcgaggaacc ccggatgcgc gaggatcttt gcgaggtgct cgcggttcgt
     2281 gacgacgccg ggcacccacg tctcgtcgag cgcgcgacgt agtacctggg ctgcttcgcg
     2341 gcgcgtcggc gcgtgtgcga tcagcttgcc gagcatcgag tcgtagtgga tgccgatttc
     2401 gctgccggcg acgaccccga tatcggcgcg tatcgtcgac ggaacatcga ccgcgtgcag
     2461 cgtgccggtt gtcggcaagt agtcgcgctc cggatcctcg gcacacagcc ggacctcgat
     2521 cgcccagccc tgctgcggtg gcgccgccgc gtatccgagg cgttctccgc gcgcgatgcg
     2581 aatctgctcg cgcacgagat cgagcccggt cgtcagctcg gtgaccggat gctcgacctg
     2641 gagccgcgtg ttgacctcga ggaagtagaa gttgccctgc gggtccgcga tgaactcgac
     2701 agtgccggcg ccgacgtaac cgactgcgcg accgagcgcg accgctgcat cacccatcgc
     2761 ggcgcgcttc tgcggatc
//
LOCUS       DASUFX010000817         9628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1662511, whole genome shotgun sequence.
ACCESSION   DASUFX010000817 DASUFX010000000
VERSION     DASUFX010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662511"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFV99_32985"
     CDS             complement(<1..485)
                     /locus_tag="VFV99_32985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064252.1"
                     /translation="MVVPGGGTVGVGTGVDGYSGTALAGGGCPVGGWPVGGWPVGGWP
                     IGGWPVGGWPVGPVSQVGCGCPVAGCPVGGCPVAGCPVAGCPVAGCPVGGCPVASCPV
                     AGCPVAGCPVGGCPVAGCPVAGCPVAGCPVGGCPVAGCPVGGCPVGGCPVGGCPVGGC
                     PV"
     gene            490..1677
                     /locus_tag="VFV99_32990"
     CDS             490..1677
                     /locus_tag="VFV99_32990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064253.1"
                     /translation="MPTLYPSTPVVLPGTVPVLSDHSYSIFDHLFYPAVRSPTLLLAG
                     QVPHLGLVLGGADRLEIQRWMLSGYWQPGNDTHKSHYAAAAGYVNNMLAPVSVIAQGS
                     FIDWRSQSIDDTDPMNTITYDEEHRTRDASAAISYTYRGSLFTTLGGVYTDDFAQVDT
                     LPSSRLHLGGPSATVEWYSAESTRYTGPRRALLANAAIAYYPRQWSTFMGNITDVAGT
                     LGTTLPLPFGRRHTISAFARARSLLAPDDTGLLQVGGDSALGILWNRSNKMEPPDFDT
                     SRFPPNLRFVEPLRGFEDFGIAAERVKLGEVSWKYPLIIDRGTAASLFVLPASFLRQL
                     DLELFASGAIVDASDRHYAVGAATTLHIQFLRIPLVVMYQIARRLTDDEALTHLIAIG
                     PDI"
     gene            complement(1651..2361)
                     /locus_tag="VFV99_32995"
     CDS             complement(1651..2361)
                     /locus_tag="VFV99_32995"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEX5064254.1"
                     /translation="MRTLVLVAALATACSSYRGTAHDTSPAKLAREPGWVLLRDVPYV
                     QQDTEYECGAAAISMVVSYWTGVETRRVVAHFRPVGKNGIAAGKLRDYAREQGLASYV
                     IAGKVEDLARELEAGRPVLVGLTKPVSRGKVLYHYEVVVGLHRDKQRIVTLDPDKGWR
                     ENSLEGFASEWKHEGFVTLIVSAKPAAAASAPASAASVPVPAAQGPAASGPAASGPAA
                     AASAASASTASDVRADRD"
     gene            complement(2361..2633)
                     /locus_tag="VFV99_33000"
     CDS             complement(2361..2633)
                     /locus_tag="VFV99_33000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064255.1"
                     /translation="MSKLAICLVTLCMLGSPVLATPTPTDEAPAVVEKSPAKKAASSD
                     ELQQYAQREHAAEKLAKFEGGRGGNDTLVTVILVLVIVILVLAIVH"
     gene            complement(2679..3380)
                     /locus_tag="VFV99_33005"
     CDS             complement(2679..3380)
                     /locus_tag="VFV99_33005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064256.1"
                     /translation="MRAIVLMCVLVVGCWRNASKREPAPIVVEEHPPLTVEPKRDGGL
                     YAMDEALADVFSGPLEHIGTGPWFGLFRINACAYRNKRVVVVNIYCTTKEMKAFSIVV
                     FSPTRGRVMIYAEADKPISQLRRGDYFTFKGESSPPGDPATLPLLSLDWSYEKLTAWD
                     KKRYYKWLPGCFGGVEIRKPQGGCLKALEAKLDKFKTRNQPFFDEPPDDWYRVVREMR
                     ARAVRDGKFIAHPGG"
     gene            3542..4252
                     /locus_tag="VFV99_33010"
     CDS             3542..4252
                     /locus_tag="VFV99_33010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064257.1"
                     /translation="MVALLICASAGAAYAQAEHYEPIRVDTGISGTYTSASGRGGFGA
                     VVEPKFLLHDHVGIGARFEGAVQFGGSFGNDDISMSVAALAATLVKGEYYPTLGTARP
                     WVGLAFGMYDIVSQDVSTGMSMATVDQKAGRYFGLAPQLGVDLGRLRLAATYNMILGA
                     DIEIRQTVGGAPQSTSYSQSYFTFEMSFRFGGRRKSPPPHIEAPPPPAPMPAPAPAAS
                     PAAPDAAAPVPPAPTPTT"
     gene            complement(4391..5548)
                     /locus_tag="VFV99_33015"
     CDS             complement(4391..5548)
                     /locus_tag="VFV99_33015"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5064258.1"
                     /translation="MGVFASGCGIDDGEQYSQVESFATVTSYETSTCSTSVVIGLSKQ
                     IADEIGCASPTGLVKFTAGGKLTITSNSVLPYLAANAKQDLMDVAANNTVQVNSAFRT
                     VAQQYLLYRWYQLGRCGITAAATPGNSNHESGRALDIANYSSIISAMGGQGWSHDVPN
                     DPVHFDHLSSPDIRGRDVLAFQKLWNRNHPTDAIAEDGDYGPMTEARLKKAPSEGFAI
                     GPTCLQGTRDLDVVSVNGPDRASPQTRVHYAITVKNAGTANWPATTKLQLKSATSSQL
                     YDESWTSQTVITTLGAAIEAGKSGTIDLDVTTPATDVDLPIMEELTLNDGGTKFGDIQ
                     LALTVVPGMSGDESGDGNEMPDPADDGGCNAGGNASWFALLALVGLRRRRR"
     gene            5658..6560
                     /locus_tag="VFV99_33020"
     CDS             5658..6560
                     /locus_tag="VFV99_33020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5064259.1"
                     /translation="MRLLFCGSGWLPIVDAIEARLPAGTTITCWDRTQPLTEVVRDVD
                     VLLPSNAHITPDVIAAATKLRLIQQPAAGTEKIDREAALARGIPICNAPGTNHVAVAE
                     LALYLLLALARRAPVAARTFAGREVGVPLGIELAGRTLGIIGMGRNGTAFADRARALG
                     MTVIALGRDATPEQRRTFFAACDAVSIHCPLDPSTRGLVGAEAFAAMKPGTFVINVSR
                     GGVIDRDALLAALATGRLGGVGLDVHWEEPADPADPLYADPHVVALPHIAGSTQEAFA
                     RITDVVVENLTRLSRGDQLLNRVV"
     gene            6612..7250
                     /locus_tag="VFV99_33025"
     CDS             6612..7250
                     /locus_tag="VFV99_33025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX5064260.1"
                     /translation="MSWLVRYLRSSVGAKHVMAVTGLLLLLFAIFHMLGHLQMFGGRE
                     MYNGYANFLQRLWEVKWPVRVGLLGLLILHVVIALALVAKNRAARPIEYAVYRPQRSS
                     IVGRSMALSGLVVLAFLIFHIIDMTIDPAIEHNAYSLYVAAFQVPWHYAVYLVGILLL
                     AMHLGHGASSWLQSLGWRHPKYPADRIGPLVAVGLFVGYMIPPTAVLVGVIS"
     gene            7247..9154
                     /locus_tag="VFV99_33030"
     CDS             7247..9154
                     /locus_tag="VFV99_33030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX5064261.1"
                     /translation="MKLDARIPAGPLEQKWANSKFEMRLVNPANKRKYKVIVVGSGLA
                     GGAAAATLAELGYDVACFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSIHRLFYDTVK
                     GGDFRSREANVHRLAEVSVDIIDQCTAMGVPFAREYGGTLANRSFGGAQVSRTFYARG
                     QTGQQLLLGVYAAMSRQIGAGKIRMFSRTEMLDLVVENGRAVGIVVRDLITGAITSHA
                     AHAVVLATGGYGNIYYLSTNAKGCNVTATYRAYKHGAGFANPCVTQIHPTCIPVAGDY
                     QSKLTLMSESLRNDGRVWVPKRKDDVAKHPDQIAEDARDYYLERKYPSFGNLSPRDIA
                     SRAAKEVCDEGRGIGPGGRGVYLDFRDAIKRDGEDRIRDKYGNLFDMYERITGENPYR
                     VPMRIYPAVHYTMGGLWVDYALMTTIPGLFCLGEANFSDHGANRLGASALMQGLADGY
                     FIIPYTIGNYLAGVPLGSPTDSTTAAFRDAETEVRARLDRLMNANGKRSVDTFAKELG
                     HIMWEHCGMTRSRQSLETALAEIPKLREEFWREVSVLGERDSFNQSLEKAGRVADFME
                     FAELMCRDALEREESCGAHYRTEYQVDGEAKRDDEDFCHVAVWEHADKPIRHIEPLAF
                     ENVKLATRSYK"
     gene            9151..>9628
                     /locus_tag="VFV99_33035"
     CDS             9151..>9628
                     /locus_tag="VFV99_33035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX5064262.1"
                     /translation="MTAGSASSAGRGLSLTLHIWRQKDRKAKGGFVTYKVADVSTHAS
                     FLEMLDILNEQLIAKGEEPVAFDHDCREGICGMCSLMINGRAHGGRRGTTTCQLHMRL
                     FKDGDEIWIEPWRAHAFPVIKDLCVDRTAMDRVQAAGGFITANTGSAPDANDVLVSK"
BASE COUNT         1683 a         3347 c         3020 g         1578 t
ORIGIN      
        1 accggacaac cgccaaccgg acaaccgcca accggacaac cgccaaccgg acaaccgcca
       61 accggacaac cagcaaccgg acaaccacca accggacaac ccgcaaccgg acaaccggca
      121 accggacaac cagcaaccgg acaaccacca accggacaac ccgcaaccgg acaaccggca
      181 accggacaac tggcaaccgg acaaccacca accggacaac ccgcaaccgg acaaccggca
      241 accggacaac cggcaaccgg acagccacca actggacaac cggcaaccgg acaaccacaa
      301 cccacctggc tcaccgggcc gaccggccaa ccgccgaccg gccaaccgcc gatcggccaa
      361 ccgccgaccg gccaaccgcc gaccggccaa ccgccgaccg gacagccgcc acccgccagc
      421 gcggtgccgc tgtatccgtc gacaccagtg ccgacgccaa cagtgccgcc gcccggtacc
      481 accatttcgg tgccgacgct gtatccgtcg acgccggtcg tgctgcccgg gaccgtgccg
      541 gtgctcagcg accactcgta cagcatcttc gatcacttgt tctatccggc ggtccgctcg
      601 ccgacgctgc tccttgccgg ccaagtgccg cacctcggcc tcgtgctcgg tggcgccgat
      661 cggctcgaga tccagcgctg gatgctctcc ggctactggc agcctggcaa cgacacgcat
      721 aagtcgcatt acgccgccgc cgcgggctac gtgaacaaca tgctcgcacc cgtgtcggtg
      781 atcgcgcagg gcagcttcat cgactggcgc agccaatcca tcgacgacac cgatccgatg
      841 aacacgatca cgtacgacga ggagcaccgc acgcgggatg cgagcgcggc aatcagctac
      901 acgtatcgcg gctcgctgtt cacgacactc ggcggcgtct acaccgacga cttcgcccag
      961 gtcgacacgc tgccgagctc gcgcctccac ctcggcgggc cgagcgctac cgtggagtgg
     1021 tactcggccg agagcacgcg ctacaccggg ccgcgtcgcg cactgctcgc caacgctgcg
     1081 atcgcctatt acccgcggca gtggtcgacg ttcatgggca acatcaccga cgtcgccggc
     1141 acactcggca cgacgttacc gcttccgttc gggcgccggc acaccatctc ggcattcgcg
     1201 cgcgcgcgta gcttgctcgc gcccgacgac accggcctgc tccaggtcgg cggcgattcc
     1261 gcactcggca ttctgtggaa tcgctcgaac aagatggaac cgccggattt tgacacgtcg
     1321 cggtttccgc cgaacctgcg cttcgtcgag ccgctgcgcg ggttcgaaga tttcggcatc
     1381 gccgcggagc gcgtgaagct cggcgaggtg tcgtggaagt acccgctgat catcgatcgg
     1441 ggtaccgcgg cgtcgttgtt cgtgttgccc gcgagcttct tgcgccagct cgatctcgag
     1501 ctgttcgcca gtggcgcgat cgtcgacgcg agcgatcgcc attacgcggt cggcgcggcg
     1561 acgacgttgc acatccagtt cctgcggatc ccgctcgtcg tgatgtatca gatcgcgcgc
     1621 cggctcaccg acgacgaagc gctgacgcac ctaatcgcga tcggcccgga catctgacgc
     1681 agtcgaggcc gaggcagccg aggccgctgc agccgggccc gaggcagccg ggcccgaggc
     1741 agccgggccc tgggcagccg gaaccggaac cgaagcagct gaggcaggtg cgctcgctgc
     1801 ggctgcgggc ttcgccgaca cgatcagcgt gacgaagccc tcgtgcttcc actcgctcgc
     1861 gaaaccctcg agcgagttct cgcgccaacc tttgtcgggg tcgagcgtaa cgatcctctg
     1921 cttgtcacgg tgcaaaccca cgacgacctc gtagtgatag agcaccttgc cccggctcac
     1981 cggtttcgtg aggccgacga gtaccgggcg gcccgcctcg agctcgcggg cgagatcctc
     2041 gaccttgccg gcgatcacgt aggacgcgag gccttgctcg cgcgcgtagt cgcgcagctt
     2101 gcccgcagcg attccgtttt tgccgaccgg acggaagtgg gcgaccaccc tgcgcgtctc
     2161 gacgccggtc cagtacgaca cgaccatgct gatcgcggcg gcgccgcatt cgtactcggt
     2221 gtcctgctgg acgtacggga cgtcgcgcag cagcacccag cccggctcgc gcgcgagctt
     2281 ggccggcgac gtgtcgtgcg ccgtgccgcg atacgacgag cacgctgtcg ccaatgcggc
     2341 gaccagaacg agcgtgcgca tcagtgcacg atcgcgagca cgagaatcac gatcacgaga
     2401 acgagaatga ccgtgacgag cgtgtcgttg ccgcctctgc caccctcgaa cttcgcgagc
     2461 ttctcggcgg catgctcgcg ctgcgcgtat tgctgcagct cgtcggagct cgcggccttc
     2521 ttggccggcg acttctcgac gacggcgggt gcctcgtcgg ttggcgttgg tgtcgcgagc
     2581 accgggctcc cgagcatgca cagcgtgaca agacaaatgg ctagcttgct catgcgacta
     2641 tcgtaacccg gaaacgtcac ccgccgggat gtgcgatgtc agccgccggg atgcgcgatg
     2701 aacttgccat cgcgcactgc acgcgcacgc atctcgcgca ccacgcggta ccagtcgtcg
     2761 ggaggttcgt cgaagaaggg ttgattgcgc gtcttgaatt tgtcgagctt cgcttcgagc
     2821 gccttcagac atccgccttg tggcttacgg atttcaacgc caccgaaaca gcccgggagc
     2881 cacttgtagt agcgcttctt gtcccacgcg gtgagcttct cgtagctcca gtcgagcgag
     2941 agcagcggca gtgtggccgg gtcgccaggc ggcgagctct cgcccttgaa cgtgaagtag
     3001 tcgccgcgcc ggagctgact gatcggtttg tcggcttcgg cgtagatcat cacgcggccg
     3061 cgcgtcggcg agaacacgac gatgctgaac gccttcatct ccttcgtcgt gcagtagatg
     3121 ttgacgacga cgacgcgctt gttgcgatac gcgcacgcgt tgatccggaa caagccgaac
     3181 cacggcccgg taccgatgtg ctcgagcggc cccgagaaca cgtcggcgag cgcttcgtcc
     3241 atcgcataga gcccaccgtc gcgcttcggc tcgaccgtca gcggcggatg ttcctcgacc
     3301 acgatcggtg cgggctcgcg cttggacgca ttgcgccagc agccaaccac caacacacac
     3361 atcaggacga tcgcgcgcat gtactcgatg attacgcacg agtatccgag atgcgtcagc
     3421 gtgaacgcca aatgaccggg gtcaggcact acttctacta cttgcctttg caggagcccg
     3481 tggtccctcc gttgcactgg ccctaagagc cgtctggggg acttctatga gctcacgtag
     3541 cttggtggct ttgcttatct gtgcgagcgc cggcgcggca tacgcgcagg ccgaacatta
     3601 cgagccgatt cgtgtcgaca ccggcatctc cggcacgtac acgagcgcga gtggtcgcgg
     3661 cggattcggc gccgtcgtcg aaccgaagtt cttgctgcac gaccacgtcg gcatcggcgc
     3721 gcgcttcgaa ggtgccgtgc agttcggcgg cagcttcggc aacgacgaca tctcgatgag
     3781 tgtcgccgcg ctcgccgcga ccctggtcaa aggcgagtac tacccgacgc tcggcactgc
     3841 acgaccgtgg gtcggtctcg cgttcggcat gtacgacatc gtcagtcagg acgtgtcgac
     3901 gggcatgagc atggcgaccg tcgatcaaaa ggccggacgc tatttcggcc tcgcgcccca
     3961 gctcggcgtc gacctcgggc ggctacgcct cgccgcgacc tacaacatga tcctcggcgc
     4021 cgacatcgaa atccgccaga cggtcggcgg tgcgccgcaa tcgacgtcgt actcgcagag
     4081 ctacttcacg ttcgagatgt cgttccggtt cggcggccgt cgcaaatcgc caccgccgca
     4141 tatcgaggct ccgccgcctc ctgcaccgat gccggcacct gcaccggccg catcgccggc
     4201 agcaccggat gcagctgcgc cagtgccacc agcaccgacg ccgacgacct agcgggcggg
     4261 cacgacggac tccgacgcgc gcgacctagc ggcgacgacg acgaattcca acgagcccga
     4321 ccgcggcgac gacgaattcc gacgagcgcg agctagcgac gacgacggcg acggattccg
     4381 acgagcgcag ctagcggcga cgacgacgga gtccgacgag cgcgagcaac gcgaaccagc
     4441 tcgcgttacc acccgcattg cagccaccgt cgtcggccgg atcgggcatc tcgtttccgt
     4501 cgccgctctc gtcgcccgac atgccgggca cgacggtgag cgcgagctgg atgtcgccga
     4561 atttcgtgcc gccgtcgttg agcgtgagct cttccatgat cggcagatcg acatcagtgg
     4621 ccggcgtcgt gacgtcgagg tcgatcgtgc cggacttgcc ggcctcgatc gccgcgccga
     4681 gcgtggtgat cacggtctgc gacgtccagc tctcgtcgta cagctggctc gacgttgcgc
     4741 tcttgagctg gagcttcgtc gtcgcgggcc agttggccgt gcctgcgttc ttgaccgtga
     4801 tcgcgtagtg cacgcgagtc tgcggcgacg cgcgatcggg accgttgacc gacacgacgt
     4861 cgaggtcgcg cgtaccctgc aggcaggtcg ggccgatcgc aaagccctcc gagggcgcct
     4921 tcttcaagcg cgcttcggtc atcgggccgt agtcgccgtc ctcggcgatc gcgtcggtcg
     4981 gatggttacg gttccacagc ttctggaacg cgagcacgtc gcggccgcga atatcgggcg
     5041 acgacaggtg atcgaagtgc acggggtcat tcggcacgtc gtgcgaccag ccctgcccgc
     5101 ccatcgccga gatgatcgac gagtagttcg cgatatcgag cgcacggcca ctctcgtgat
     5161 tcgagttccc gggcgtcgct gccgcggtga tgccgcaacg gccgagctgg taccagcgat
     5221 acaagaggta ctgctgcgcg acggtgcgga acgcgctgtt gacttgcacc gtgttgttcg
     5281 cggcgacatc catcaagtcc tgcttcgcgt tcgcggcgag gtacggcaac accgagttgc
     5341 tcgtgatcgt gagcttgccg cccgcggtga acttgacgag gccagtgggg ctcgcgcacc
     5401 cgatctcgtc ggcgatctgc ttcgacaagc cgatcacgac cgacgtcgag cacgtcgatg
     5461 tctcgtagct cgtcaccgtc gcgaacgact cgacttggga atactgctcg ccgtcatcga
     5521 tgccgcagcc ggaggcgaat acccccacgc cgagcacgat gctgtgcaac atcgaggttg
     5581 cagggcgcgc gccatttcga acagttgtgc ctgtttgccc catacgcagg gcgcattcag
     5641 caagtacagt gccggtgatg cgccttctgt tttgcggctc gggctggctt ccgatcgtcg
     5701 acgcgatcga agcgcggctg cccgcaggca cgacgatcac gtgctgggat cgcacgcaac
     5761 cgctcaccga ggtcgtgcgc gacgtcgacg tgctgctgcc gtcgaacgca cacatcacgc
     5821 ccgacgtgat cgccgcagcg acgaagctac ggttgatcca gcagcccgcc gctggcaccg
     5881 agaagatcga tcgcgaagcc gctctcgcgc gcggcattcc gatctgcaac gcgccgggca
     5941 cgaaccacgt cgccgtggcc gagctcgcgc tgtatctgtt gctcgcgctc gctcgtcgcg
     6001 cgccggtcgc tgcacgcacg ttcgctggcc gcgaagtcgg cgtgccgctc ggtatcgagc
     6061 tcgccggccg cacactcggc atcatcggca tgggccgcaa cggcaccgcg ttcgccgatc
     6121 gcgcgcgcgc actcggcatg accgtgatcg cgctcggccg cgatgcgact ccagagcagc
     6181 gccgcacgtt cttcgccgcg tgcgatgcgg tgagcattca ctgccccctc gatccgtcga
     6241 cgcgcgggct cgtcggcgcc gaggcattcg ccgccatgaa gcccggcaca ttcgtgatca
     6301 acgtgtcgcg cggcggcgtg atcgatcgcg acgcgctgct cgccgcactc gcgaccggca
     6361 ggctgggcgg cgtcggcctc gacgtgcact gggaggaacc ggccgacccg gccgatccgc
     6421 tctacgccga cccgcacgtc gtcgcgctcc cgcacattgc gggctcgacg caggaagcgt
     6481 tcgcacgcat taccgacgtc gtggtcgaga accttacccg actctcgcgt ggcgaccagc
     6541 tgctgaatcg ggttgtttag cctcgccaaa cattgctgta gcgacaccac ttgtggtggc
     6601 atccaaccgt cgtgagttgg ctcgttcgct acctgcgatc gtctgtcggc gcgaagcacg
     6661 tgatggccgt gaccggcctc ctgcttctgt tgttcgcgat cttccacatg ctcggccacc
     6721 tgcagatgtt cggcggccgc gagatgtaca acggctacgc gaattttctg cagcggctat
     6781 gggaagtgaa gtggccggtg cgcgtcgggc tgctcggtct cctgatcctc cacgtcgtga
     6841 tcgcactcgc cctggtcgca aagaaccgcg ctgcgcgccc gatcgagtac gccgtttatc
     6901 ggccgcagcg ctcgagcatc gtcggccgct cgatggcgct gtcgggtctc gtcgtgctcg
     6961 cgtttctgat ctttcacatc atcgacatga cgatcgatcc ggcgatcgag cacaacgcgt
     7021 actcgctcta cgtcgccgcg ttccaggtgc cctggcacta cgcggtgtac ctcgtcggca
     7081 tcctgctgct cgcgatgcac ctcggccacg gcgcgtcgtc gtggctgcaa tcgctcggct
     7141 ggcggcatcc gaagtacccg gccgatcgca tcggcccgct cgtcgcggtc gggctgttcg
     7201 ttggctacat gatcccgcca accgcggtgc tcgtcggagt gatctcgtga agctcgacgc
     7261 gcgcattccc gccgggccgc tcgaacagaa gtgggcgaac agcaagttcg agatgcgcct
     7321 cgtcaacccg gcgaacaagc gcaagtacaa agtcatcgtc gtcggtagcg ggctcgcggg
     7381 tggcgctgcc gccgcgacgc tcgccgagct cggctacgac gtcgcgtgct tctgcttcca
     7441 ggactcgccg cgccgcgcgc actcgatcgc cgcgcaaggc ggcatcaacg ccgcgaagaa
     7501 ctaccagaac gacggcgact cgattcaccg cctgttctac gacaccgtca aaggcggcga
     7561 cttccgctcg cgcgaagcga acgttcaccg cctcgccgaa gtctccgtcg acatcatcga
     7621 tcagtgcacg gcgatgggcg tgccattcgc acgcgagtac ggcggcacgc tcgcgaaccg
     7681 cagcttcggc ggcgcgcagg tctcgcgcac gttctacgca cgcgggcaaa ccggccagca
     7741 gctgctgctc ggcgtctacg cggcgatgtc gcgacagatc ggcgccggca agatccgcat
     7801 gttctcgcgc accgagatgc tcgacctcgt cgtcgagaac ggccgcgccg ttggcatcgt
     7861 cgtccgcgat ctgatcacgg gcgcgatcac gtcgcacgcc gcgcacgccg tcgtgctcgc
     7921 gaccggcggc tacggcaaca tctattacct ctcgacgaac gcgaagggct gcaacgtcac
     7981 cgcgacgtat cgcgcgtaca agcacggcgc cgggttcgcg aacccgtgcg tcacacagat
     8041 ccatccgacg tgcatcccgg tcgccggcga ctatcaatcg aagctgacac tgatgtccga
     8101 gtcgctgcgc aacgacggcc gcgtctgggt accgaaacgc aaggacgacg tcgcgaagca
     8161 ccccgaccag atcgccgagg acgcacgcga ttactacctc gagcgcaagt acccgagctt
     8221 cggcaacctg tcgccacgtg acatcgcgag ccgtgccgcg aaagaagtct gcgacgaagg
     8281 ccgcggcatc ggccccggcg gacgcggcgt ttatctcgat ttccgcgacg cgatcaaacg
     8341 cgacggcgaa gatcgcattc gcgacaagta cggcaacctg ttcgacatgt acgagcggat
     8401 cacgggcgag aacccgtacc gcgtaccgat gcgcatctat ccggcggtcc actacacgat
     8461 gggtggcctg tgggtcgact acgccctgat gacgacgatc cccggcctgt tctgcctcgg
     8521 cgaagcaaac ttctccgacc acggcgcaaa ccgcctcggc gcgagcgcac tgatgcaagg
     8581 ccttgccgac ggctacttca tcattccgta cacgatcggc aactacctcg ccggtgtgcc
     8641 gctcggctcg ccgaccgact cgaccaccgc agcgttccgc gacgccgaaa ccgaggtgcg
     8701 tgcgcggctc gatcggctga tgaacgcgaa cggcaagcgc tcggtcgaca cgttcgcgaa
     8761 ggagctcggc cacatcatgt gggagcactg cggcatgacg cgcagccggc agtcactcga
     8821 gaccgcgctc gccgagattc caaagctgcg cgaagaattc tggcgcgagg tcagcgtgct
     8881 cggcgagcgt gactcgttca accaatcgct cgagaaggcg ggccgcgtcg ccgacttcat
     8941 ggagtttgcc gagctgatgt gccgcgatgc gctcgagcgc gaggagagct gcggcgccca
     9001 ttaccggacc gagtaccagg tcgacggcga agcgaagcgc gacgacgagg acttctgtca
     9061 cgtcgccgtg tgggagcacg ccgacaaacc gattcgtcac atcgagccgc tcgcgttcga
     9121 gaacgtcaag ctcgcgacga ggagttacaa gtgacagccg gctctgcaag ctccgccgga
     9181 cgtggtctgt cgttgacgct ccacatctgg cgccagaagg accgcaaggc gaagggcggc
     9241 ttcgtcacgt acaaggtcgc cgacgtgtcc acgcacgcgt cgttcctcga gatgctcgac
     9301 attctcaacg agcagctgat cgcaaaaggc gaagagccgg tcgcgttcga tcacgactgc
     9361 cgcgaaggca tctgcggcat gtgctcgctg atgatcaacg gccgcgcgca cggcggccgc
     9421 cgcggcacga cgacgtgcca gcttcacatg cgcctgttca aggacggcga cgagatctgg
     9481 atcgagccgt ggcgcgccca cgcgttcccg gtgatcaagg acctgtgcgt cgaccgcacc
     9541 gccatggacc gcgtccaggc ggccggtggc ttcatcaccg ccaacaccgg ctcggcgccg
     9601 gatgccaacg acgtcctggt ctccaagg
//
LOCUS       DASUFX010000818         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_273306,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000818 DASUFX010000000
VERSION     DASUFX010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273306"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV99_33040"
     CDS             complement(<1..336)
                     /locus_tag="VFV99_33040"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5064263.1"
                     /translation="MDLRLSWESLPLMVPLSDGGNVIVEYQRPSTNVDGIDRVALVAS
                     VDDQPVAVARLIELPGDAEIRFRVDEDYRRRGIGSILRDELLAIAHARGIYRFHANVV
                     PDNVAIRRLL"
     gene            complement(400..1119)
                     /locus_tag="VFV99_33045"
     CDS             complement(400..1119)
                     /locus_tag="VFV99_33045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064264.1"
                     /translation="MRALWILVVAGCHSADSPITLVDAAPGPVGYTLTVGWTDPQDKP
                     PPDPKPVIFIDGVATQQLLLEYPSPADARAAEHVIELRHGDTVVYTQVSSNTSRGCLA
                     QVPDATMVLEGWCAYANGEVRFGSDQANGTNMFCIGDGFCRPVCIPGSCAAGTHCTSI
                     YASTNPVATHLGCAPIGTQQPDAACTLVTDASGTHDDCGSGLLCVGGTCKRLCNPYNP
                     PLPNCTCTYLPEHAPELSVCL"
     gene            1149..2492
                     /gene="allB"
                     /locus_tag="VFV99_33050"
     CDS             1149..2492
                     /gene="allB"
                     /locus_tag="VFV99_33050"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX5064265.1"
                     /translation="MTDLVVRSQRVVMPDGIRAATVHVTAGTIERVTAWDDVPAGARV
                     VDYGELAVMPGIVDTHVHLNEPGRTEWEGFATATRAAAVGGVTTLVDMPLNSIPPTTT
                     REAFAAKRDAARGQCAVDVGFWGGVVPGNQRELAGLVADGVRGFKCFLVDSGVAEFGW
                     VGEPELAPAMQILAGLNVPLLVHAEVAGPIDAATAQLAKADPRRYATYLASRPPAAEE
                     QAITLVTRLCRATNARTHIVHHSAATALPLLRQARADGLPLTAETCPHYLHFTAEAIP
                     DGATPFKCAPPIRDADNREALWRALAEGVLDFVASDHSPCSPSLKAMEVGDFVAAWGG
                     VAGLQLALPVVWTEASRRGHTLVDLARWMSAGPARLAGLTGKKGAIVAGGDADFVVFA
                     DAETTVVTPESVQHRHKVTPYAGETLRGAVHATYLRGQLVAERGRALATDQGALL"
     gene            2489..>2941
                     /locus_tag="VFV99_33055"
     CDS             2489..>2941
                     /locus_tag="VFV99_33055"
                     /inference="COORDINATES: protein motif:HMM:NF015522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064266.1"
                     /translation="MSDFTELPDLACEAIGGAAILCNDEFFAEKENMLRAHDAVWKEH
                     EYTDRGKWMDGWETRRYRPADGRAPGRDSEVHDWCVVRLGLPGVIKGVVVDTAFFRGN
                     YPDTCALYGATIDDALDLRALEHATWVPLVPRSPLEGNAKNKFSVLSPE"
BASE COUNT          494 a          937 c         1028 g          482 t
ORIGIN      
        1 gagcaagcgg cggatcgcga cgttgtcggg cacgacgttc gcgtggaaac gatagatgcc
       61 gcgtgcgtgt gcgatcgcga gcaactcgtc gcgcaggatc gacccgatgc cgcgacgccg
      121 atagtcctcg tcgacgcgga agcggatctc ggcatcgccg ggcagctcga tcagccgcgc
      181 gacggcaacg ggctgatcgt cgaccgacgc gacgagcgcg acgcggtcga tgccatcgac
      241 gttggtcgac ggacgctggt actcgacgat gacgttgcca ccgtcggaga gcgggaccat
      301 gagcgggagg ctttcccacg acaggcggag gtccatgcgc agaagcatga ccgacgaact
      361 tttcgtcggc gtttcgtgaa catcacttct tcgcgacggt cagagacaga cactgagctc
      421 gggagcgtgt tcaggaagat acgtgcacgt gcagttggga agcggcgggt tgtaaggatt
      481 gcatagccgc ttacaggtcc cgccgacaca caacaaacca gatccgcaat catcatgagt
      541 gccgctcgcg tcggtgacga gcgtgcacgc cgcgtcgggt tgttgtgtgc cgatcggagc
      601 gcagccgaga tgcgtcgcaa ccggattcgt cgatgcatag atgctcgtgc agtgcgtgcc
      661 tgcagcgcag ctaccgggta tgcacaccgg cctacaaaag ccgtcgccga tgcaaaacat
      721 gttggtgccg ttcgcctggt ccgagccgaa tcgaacctcg ccgttcgcgt acgcgcacca
      781 gccttcgagg accatcgtgg cgtcgggcac ctgagcgagg cacccgcgcg acgtgttgga
      841 cgagacctgc gtgtacacga ccgtgtcccc gtgccggagc tcgatgacgt gctcggctgc
      901 gcgcgcatcg gctgggctcg gatactcgag cagcagctgt tgtgtcgcga caccgtcgat
      961 gaagatcacc ggcttcggat cggggggcgg cttgtcttgt ggatccgtcc agccgaccgt
     1021 cagcgtgtag cccacgggcc cgggggctgc atcgacgagc gtgatcggcg agtcggcgct
     1081 atggcagccg gccacgacga ggatccacag cgcacgcacc acatgagtat gcggtaatct
     1141 gcggcgcagt gactgacctc gtcgtccgat cgcaacgcgt cgtgatgccc gacggtattc
     1201 gtgcggccac cgtgcacgtg acggccggca cgatcgagcg cgtcaccgcg tgggacgatg
     1261 tgccggcagg tgcgcgcgtc gtcgactacg gcgagctcgc tgtgatgcct ggcatcgtcg
     1321 acacgcacgt ccacttgaac gagcccggcc gcaccgagtg ggagggcttt gcgaccgcaa
     1381 ctcgcgcggc cgcggtcggc ggcgtcacca cgctcgtcga catgccactc aactcgatcc
     1441 caccgacgac gacgcgcgaa gcgttcgcgg cgaagcgcga tgcagcgcgc ggtcagtgcg
     1501 cggtcgatgt cggcttctgg ggcggcgtag tgccgggcaa tcagcgcgag ctcgcagggc
     1561 tcgtcgccga cggcgtgcgc ggcttcaagt gtttcctcgt cgacagcggc gtcgccgagt
     1621 tcggctgggt tggagagccc gagctcgcgc ccgcgatgca gatccttgca ggcctgaacg
     1681 tgccgctact cgtgcacgcg gaagtcgcgg gcccgatcga cgcggcgact gcacagctcg
     1741 cgaaggctga tccgcggcgc tacgcgacgt atctcgcgtc gcgaccaccg gcagccgaag
     1801 agcaagcgat cacgcttgtc acgcggctgt gtcgcgcgac gaacgcgcgc acgcacatcg
     1861 tgcatcactc ggccgcgacc gcgttgccgc tgttgcgtca ggcgcgcgcg gatggcttgc
     1921 cgttgaccgc ggagacgtgt ccgcattact tgcacttcac ggccgaggcg attcccgacg
     1981 gcgcgacgcc gttcaagtgc gcaccgccga ttcgcgatgc cgataatcgc gaggcgctgt
     2041 ggcgcgcgct cgccgaaggc gtcctcgatt ttgtcgcgag cgatcactcg ccgtgcagtc
     2101 cgagcttgaa ggcgatggag gtcggtgact tcgtcgccgc gtggggcggc gtcgccggat
     2161 tgcagctcgc gctgccggtc gtgtggaccg aagcgagccg gcgcggtcac acgctcgtcg
     2221 atctcgcgcg ctggatgagt gcaggaccgg cgcgcctggc tggactcacc gggaagaagg
     2281 gcgcgatcgt cgccggcggc gatgccgact tcgtcgtgtt tgccgatgcc gagaccaccg
     2341 ttgtcacgcc ggagagcgtg cagcaccgtc acaaggtgac gccgtatgca ggcgagacgc
     2401 tgcgcggtgc ggtgcacgcg acgtatttgc gcggccagct cgtcgccgaa cgcggccgcg
     2461 cgctcgccac ggatcaagga gcgctgttgt gagtgacttt accgagctgc cggatctcgc
     2521 gtgcgaggca atcggcggtg cagcgattct gtgcaacgac gaattcttcg cggagaagga
     2581 gaacatgctc cgcgcgcacg acgccgtgtg gaaagagcac gagtacaccg atcgcggcaa
     2641 atggatggac ggctgggaga cgcgacgcta tcggcccgcc gacggccgcg cgccgggtcg
     2701 cgacagcgag gttcacgact ggtgcgtcgt gcggctcggt ttgccgggcg tgatcaaagg
     2761 cgtcgtcgtc gacaccgcgt tcttccgcgg caactatccc gatacgtgcg cgctatacgg
     2821 cgcgacgatc gacgatgcgc tcgatctgcg agcgctcgag cacgcgacct gggtaccgct
     2881 cgtgccgcgc tcgccactcg aaggcaacgc gaagaacaag ttctccgtgc tgtcgccgga
     2941 g
//
LOCUS       DASUFX010000819         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_828048,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000819 DASUFX010000000
VERSION     DASUFX010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828048"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFV99_33060"
     CDS             complement(<1..390)
                     /locus_tag="VFV99_33060"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5064267.1"
                     /translation="MDRARLETLAYTALRVVTGFMFFFHGSQKLLGFPGGKVAPESFS
                     QMWIGGIIELVCGALIALGLLTRAAAFLASGMMAVAYMQFHWKGHFGADFLPIVNKGE
                     LAVVYCFVFFFFMCAGAGGLSVDSQLGK"
     gene            complement(492..1862)
                     /locus_tag="VFV99_33065"
     CDS             complement(492..1862)
                     /locus_tag="VFV99_33065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5064268.1"
                     /translation="MATILIIDDNDTIREGLAITVKKLGHDPITAANGAAGVTAFKQR
                     RADFVITDLKMEGQTGVDVLKAISAIDPDVPIMIITGFGTVETAVEAMKLGAFDFITK
                     PIQTEVVRLKVERALEVCATKRGRRKADAQVDYLRAESDSKFAELVGGGDKMAQVRRT
                     VEKVAASDTTVFIAGESGTGKELVARAIHRLSKRASGPFIKVNCGALTETLLESELFG
                     HEKGAFTGAIKQKLGRFELADGGTLFLDEVGDVPPSMQVKLLRALQEQEFERVGGEAP
                     IKVDVRVVSATNKDLEAEVNAGRFRQDLFFRLHVLPVKLPALRERREDIPLLVQHFIA
                     KLGPKTNPRVRSISDAALGRIMAYHWPGNVRELENAIEQSLVFAEGEEIGPPSLPQFL
                     QGGAEEDALNVPREMSLPEILDDLERQLILKAYAKAGRVKTETARLLGIKTSALYYKL
                     EKYGIE"
     gene            2202..>2721
                     /locus_tag="VFV99_33070"
     CDS             2202..>2721
                     /locus_tag="VFV99_33070"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5064269.1"
                     /translation="MNDVVREQLCFALSDQAERARWRMDDIAWDRVDPAKAPEPLRML
                     VKEAAFAELTTTSATRRFLDDLADDTDFTQWVSVWFFEETRHPQVLVRWLHQLGITVD
                     ARFMRRGRATAPLMKSRIGTLVANVISELVASASYANLAVRAEEPVLCDIARHLAADE
                     ARHAASFYAYARR"
BASE COUNT          448 a          865 c          913 g          495 t
ORIGIN      
        1 cttgccgagc tgcgagtcga cgctcaaccc gccggcgccc gcgcacatga agaagaagaa
       61 cacgaagcag tacacgaccg cgagctcgcc cttgttgacg atcggcagga agtcggcgcc
      121 gaagtgaccc ttccagtgga actgcatgta cgcgacggcc atcatgcccg acgcgagaaa
      181 cgcggcagcg cgcgtgagca ggccaagcgc gatcagcgcg ccgcagacga gctcgatgat
      241 gccgccgatc cacatctgcg agaacgactc cggggcgacc ttgccgccgg ggaacccgag
      301 cagcttctgc gagccgtgaa agaagaacat gaagccggtg acgacgcgca gtgccgtgta
      361 cgccagcgtt tcgagtcgtg cgcgatccat ggcgaacatc tagcacgcaa gccgacgctg
      421 acacgtgtaa cgaagtgttt cgctcggcgg tgcctgaggc acatcgatgt gcgttaccgc
      481 tcgggtcggc cctactcgat cccgtacttc tcgagcttgt aatagagcgc actcgtcttg
      541 atgccgagca ggcgcgcggt ctcggtcttc acccgaccgg ccttcgcata ggccttgagg
      601 atcagctggc gctcgagatc gtcgaggatc tcgggaagcg acatctcgcg aggtacgttg
      661 agcgcgtctt cctcggcacc accttgcagg aactgcggta gcgacggtgg tccgatttct
      721 tcgccctcgg cgaatacgag cgattgctcg atcgcgttct cgagctcgcg cacgttgccc
      781 ggccagtgat aggccatgat gcgaccgagc gcggcgtcgc tgatcgagcg tacccgcggg
      841 ttcgtcttcg gaccgagctt ggcgatgaag tgctggacga gcagcgggat gtcttcgcgg
      901 cgctcgcgca atgccggcag cttcaccgga agcacgtgaa ggcggaagaa caggtcttgc
      961 cggaagcggc cggcattgac ctcggcttcg agatccttgt tggtggcgga aacgacgcga
     1021 acgtcgactt tgattggcgc ttcgccgccg acgcgctcga attcttgctc ctgcagcgca
     1081 cgcagcagct tgacctgcat cgacggcggc acatcgccga cctcgtcgag gaacagcgtg
     1141 ccgccgtccg cgagctcgaa gcgaccgagc ttctgcttga tggcgccagt gaatgcgccc
     1201 ttctcgtgac cgaacagctc gctctcgagc agcgtctcgg tgagcgcgcc gcagttgacc
     1261 ttgatgaacg gcccgctggc gcgcttgctc aggcgatgga tcgcgcgcgc gacgagctcc
     1321 ttgccggtgc cgctctcgcc ggcgatgaac accgtggtgt cgcttgccgc gaccttctcg
     1381 acggtacggc gaacctgagc catcttgtcg ccaccgccga ccagctcggc gaacttgctg
     1441 tcgctctcgg ctcgcaggta atcgacctgg gcgtctgctt tgcggcggcc gcgcttcgtc
     1501 gcgcacactt cgagcgcgcg ctcgaccttg aggcgtacga cctcggtctg gatcggctta
     1561 gtgatgaagt cgaacgcgcc gagcttcatt gcctcgacgg cggtctcgac cgtaccgaag
     1621 ccggtgatga tcatgatcgg gacatcggga tcgatcgcgc tgatcgcctt gagcacgtcg
     1681 acgccggtct ggccctccat cttgaggtcc gtgatcacga agtcagcgcg gcgctgcttg
     1741 aacgcggtga cgccagccgc accatttgcg gccgtgatcg ggtcatggcc gagcttcttg
     1801 accgtgatcg cgaggccctc acggatcgtg tcgttgtcgt cgatgatgag gatcgtcgcc
     1861 atgtccgcgg ggcccatggt agccgctcgc cggagcgcgt ggcggcggat ccgatcaaga
     1921 tccggcagaa acggaacatc gtcgtctccg agagggtggg atcactcgcg ctgctgttgt
     1981 gagctgcatg cgagtgtggt tggcttcgag ggtggagcac gtcagggtcg acctgaaggt
     2041 ctgcgtacgg tgtgctgcgt gcgcgatcgt ggcgccgcac acgttcgagc tcacccggaa
     2101 aggtactcgc gtggtccgcc agcccagcga tgatgccgag cgtgcagcat gtcgggcggc
     2161 ggcactgctg tgcccgacac gcgcgatcga ggtgggcccg catgaatgac gtcgtccgcg
     2221 agcagctgtg cttcgcgcta tccgatcagg ccgagcgtgc gcgctggcgc atggacgaca
     2281 tcgcgtggga tcgcgtcgat ccggcgaaag cgcccgagcc cttgcgcatg ctggtcaagg
     2341 aagccgcgtt cgcggagctg acgacgacgt cggcgacgcg gcggtttctc gacgacctcg
     2401 ccgacgacac cgacttcacg cagtgggtct cggtgtggtt cttcgaggag acgcgacatc
     2461 cgcaggtgct cgtgcggtgg ctgcatcagc tcggcatcac cgtcgatgct cggttcatgc
     2521 gtcgcggccg tgcgacggcg ccgctgatga agtcgcgcat cggcacgctc gtggccaacg
     2581 tgatctcgga gctcgtcgcg tcggcgtcgt acgcgaacct cgcggtgcgc gccgaggagc
     2641 ccgttttgtg cgatatcgcg cggcatctcg ccgccgacga agcgcggcac gcggcgagct
     2701 tctacgcgta tgcccgccga t
//
LOCUS       DASUFX010000820         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1385050, whole genome shotgun sequence.
ACCESSION   DASUFX010000820 DASUFX010000000
VERSION     DASUFX010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385050"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="VFV99_33075"
     CDS             complement(<1..2003)
                     /locus_tag="VFV99_33075"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEX5064270.1"
                     /translation="MHEHTIPESLIEQIRTGKAALVVGAGIGVPSWKQLLERLTKALE
                     TRGREGDDAATKDLEKLLHKGSLVRAVGFLARALGEETCDRIVQETWAAPAEVPALPK
                     QLAKLPFRHVWTTFPGDVLEHALETGSPTEWPPARVVSYQELGELSLRRRTLVKMLGN
                     FDTYVVTPASVRRALSRAVDLREYARELYVEGSLVFVGFRYGDPDLSALLDRVFGMFE
                     PPRGTHYFLGAGVGPVTVDELMADHHIEVVNLAGRGGDDVAERSVIEWLESLRAACER
                     VQITLAQTRPDQDDVDGWVAMLAEGGEEAAEARDALALIERKARAAKKWDTVVEVLLG
                     RVEAAEAGERANALLELSGVYETEVGDLRRAFEAATTACQLAPEDDVAAARAEKLAAS
                     TGGWADLVNEASEIATDQQDPKLASKWWARLGGWYAQKLDRADYATPSLRRALELDNQ
                     NRAAHLAIAEMYRKQQKWADLADALKAHGEVETDIQTKVGLLIDLGDLYETQLASTAK
                     AVESYQHAFDLDENSDDALAALERLYRRDEKWANLAKVLDRRAEISAESGDQGRAAAI
                     RRELATLRAEKLGDLEGAIQRYEAAVAANGSDAEALKALVDLYDKTGRTEDYLRTMER
                     LGAVAPEGEKLATLRKLAAELEDRDPARAGAAYDQLIAADPNA"
     gene            2090..3742
                     /locus_tag="VFV99_33080"
     CDS             2090..3742
                     /locus_tag="VFV99_33080"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5064271.1"
                     /translation="MRLRRKITIAFFLVSSLVSVLLALFLYRFIEHQLESELRSKLLD
                     MAHIGAAEIDVEAYRRLHDQLQRGAADDAKVAAVEASEDYLAISAQLRMLRATEPALM
                     QYAYLLAPTDDPANPAFVVDADVLDFNAKLAHGEKLPEGESVSHFNLPYDVSALPVLA
                     KALAECSTHQEKDFVYDAKFKVSSVSAYAPLSDRTGTPLRDSKGRCLGVLGLDITDRQ
                     MRAALANAGGLAIKISLAMIVLALIVSIALSTLLTRSILALTSTVKRFADKDFTARTP
                     ILSKDEVGQLGENFNAMAATIQEHNDNLEELVKKRTKELSDEKQTSERLLLNVLPGPI
                     ADRLKSGEGLIVDRFEAVSVLFADIVGFTSLSSRIPPEQLVTMLNELFSTFDKLAEKH
                     GLEKIKTIGDAYMVVAGIPQPVANHAHAIAHMALDMLLAVDDFAKRHASDLTIRIGIH
                     TGPVVAGVIGQKKFIYDLWGDTVNTASRMESHGVPGKVHVTATTFAALRDTFELEPRE
                     PIEIKGKGRMQTYLIVGKRATMKSLEPRPSDPRGWGSETDGG"
     gene            3739..4656
                     /locus_tag="VFV99_33085"
     CDS             3739..4656
                     /locus_tag="VFV99_33085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064272.1"
                     /translation="MTSKLRWSAPAWALFTTRHATETLADDLPLAVTVEQMGPARILH
                     LGIGKGKVSIAIALGKKPNATFTVRTAAEGAACVDALATWLGVPVPPPSTGAVAMPPP
                     VTVGVIDMGRGKGQRHVWQLVQIVLGYEARFYLIWRVAGTEAFLCEHESGNRESLIAE
                     LAAVLRDGTREVSAAQPTNGHYFVFDGERSNVDTRAWARKLHLEKADVERRLEAAGRA
                     HVDPLYAQIETALDAWPEDPRGAATRVMKIPVYGCVGAVWGSIAHRDPATLSTQEVAA
                     LHQKLRSLAAADYVQRLYHQDEVHALLLR"
     gene            4665..>4849
                     /locus_tag="VFV99_33090"
     CDS             4665..>4849
                     /locus_tag="VFV99_33090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064273.1"
                     /translation="MWSDVAAIYRSSKKKKDINWFTEWIARPPGAVVVYFLRNTRVTP
                     NQITFASAFIAAGACAM"
BASE COUNT          798 a         1635 c         1603 g          813 t
ORIGIN      
        1 tcggcgttgg ggtccgcggc gatcagctgg tcgtacgcgg cgcccgcgcg cgcgggatcg
       61 cgatcctcga gctcggcggc gagcttgcgc agcgtcgcca gcttctcgcc ctcgggcgcg
      121 acagcgccga gccgctccat cgtacggagg tagtcttccg tccggccggt cttgtcgtag
      181 agatcgacga gcgccttgag cgcttcggcg tcgctgccgt tcgcggcaac ggcggcctcg
      241 taacgctgga tcgcgccctc gaggtcaccg agcttctcgg cgcgcaacgt cgccagctcg
      301 cgacggatcg cggcggcgcg gccctggtct cccgattccg cgctgatctc ggcgcggcga
      361 tcgagaacct tggcgagatt cgcccacttc tcgtcgcggc gatacagacg ctcgagcgcg
      421 gcaagcgcgt cgtcgctgtt ctcgtcgaga tcgaacgcgt gctggtacga ctcgacggcc
      481 ttcgcggtcg acgcgagctg cgtctcgtag aggtcgccga ggtcgatgag caggccgacc
      541 ttggtctgga tatcggtctc gacttcgccg tgcgccttca gcgcgtcagc gaggtccgcc
      601 cacttctgct gcttgcggta catctccgca atcgcgaggt gcgcggcgcg gttctggttg
      661 tcgagctcga gtgcgcgacg caggctcggc gtcgcgtaat cggcgcgatc gagcttctgc
      721 gcgtaccagc cgccgagccg agcccaccac ttgctggcga gcttggggtc ctgctggtcg
      781 gtcgcgatct ccgacgcctc gttgacgaga tccgcccagc caccggtcga ggcggcgagc
      841 ttctcggcgc gcgcggcggc gacatcgtcc tccggagcga gctgacacgc ggtggtcgcc
      901 gcttcgaacg cgcggcggag gtcaccgact tcggtctcgt agacacccga tagctcgagc
      961 agcgcgttcg cgcgctcgcc tgcttccgcg gcttcgacgc ggccgagcaa cacctcgacg
     1021 accgtgtccc acttcttggc ggcgcgcgcc ttgcgctcga tcagcgcgag cgcgtcgcgt
     1081 gcctcggcgg cttcttcgcc accctcggcg agcatcgcga cccagccgtc gacgtcgtcc
     1141 tgatccggac gcgtctgcgc gagcgtgatc tgcacccgtt cgcacgcggc gcgcagcgac
     1201 tcgagccact cgatcacgct gcgctcggcg acgtcgtcgc caccgcggcc agcgaggttc
     1261 acgacctcga tgtggtgatc ggccatcagc tcgtcgacgg tcaccggacc aacgccggca
     1321 ccgaggaagt agtgcgtgcc acgcggcggc tcgaacatgc cgaacacgcg gtcgagcaat
     1381 gccgacagat cgggatcgcc gtagcggaac ccgacgaaca cgagcgagcc ttcgacgtac
     1441 agctcgcgcg cgtactcgcg aaggtcgacg gcacgcgaca gcgcgcggcg gacgctcgcg
     1501 ggcgtgacga cgtacgtatc gaagttgccg agcatcttga ccagcgtgcg ccggcgcaag
     1561 ctcagctcac cgagctcctg ataactgacg acgcgggcgg gcggccactc ggtcggcgat
     1621 ccggtctcga gcgcgtgctc gaggacgtcg ccggggaacg tcgtccagac gtggcggaac
     1681 ggcagcttcg cgagctgctt cggcagcgcc ggcacctcgg cgggcgcggc ccacgtttcc
     1741 tggacgatgc gatcgcaggt ctcttcgccc agcgcgcggg ccaagaagcc gacggcgcgg
     1801 acgaggctgc ctttgtgcag cagcttctcg aggtccttgg tcgccgcgtc atccccttcg
     1861 cggccgcggg tctcgagcgc tttcgtcagg cgctccagga gttgtttcca gctcggtact
     1921 ccgattcccg caccgacgac cagggctgct ttgcccgtac ggatctgctc gatgagagac
     1981 tcggggatcg tgtgctcgtg catcctaaac ccctctgtct tgtggttcgc gcgggaccct
     2041 atcgagggca aaagacgcac gcaaggggaa agtgttaccg tcggttgggg tgcgtcttcg
     2101 acgcaagatc acgattgcgt tcttcctggt cagctcgctg gtcagcgtcc tgctcgcgct
     2161 cttcctgtac cgcttcatcg agcaccagct ggagagcgaa ttgcgcagca agctgctcga
     2221 catggcgcat atcggagcgg ctgaaatcga cgtggaggcc tatcgccgcc tccacgacca
     2281 gctgcagcgt ggagccgccg acgacgccaa ggttgccgcg gtcgaggcct ccgaggatta
     2341 cctagcgatc tcggcccagc tccgcatgct ccgcgcgacc gagccggcgc tcatgcaata
     2401 cgcctatctc ctcgccccga cggacgatcc ggcgaacccg gcgttcgtgg tcgacgccga
     2461 cgtgctcgac ttcaacgcca agctggcgca cggggagaag ctgcccgagg gcgagagcgt
     2521 ctcgcacttc aacctgccct acgacgtgtc cgcgctcccg gtgctcgcca aggcgctcgc
     2581 cgagtgctcg acccaccagg agaaggactt cgtctatgac gcgaagttca aggtgagctc
     2641 ggtctcggcc tatgcgccgc tgagcgacag gaccggcacg ccgctgcgcg acagcaaggg
     2701 gcgctgtctt ggcgtgctcg gcctcgacat caccgaccgc cagatgcgcg ccgcgctcgc
     2761 gaatgccggc ggcctggcga tcaagatctc gctcgcgatg atcgtgctcg cgctgatcgt
     2821 gtcgatcgcg ctgtcgacgc tgctcacccg ctccatcctc gcgctgacct cgacggtgaa
     2881 gcggttcgcg gacaaagact tcaccgcgcg cacgccgatc ctgtcgaagg acgaggtcgg
     2941 tcagctcggc gagaacttca acgcgatggc cgcgacgatc caggagcaca acgacaacct
     3001 cgaagagctg gtcaagaagc gcacgaagga gctatcggac gagaagcaga cgtcggagcg
     3061 gctgctgctc aacgtgctgc ccgggccgat cgcggatcgc ctgaagtccg gcgagggctt
     3121 gatcgtcgac cggttcgaag cggtcagcgt gctgttcgcg gacatcgtcg gcttcacgtc
     3181 gctgtcgtcc cgcattccgc ccgaacagct cgtcacgatg ctcaacgagc tgttttcgac
     3241 gttcgacaag ctcgccgaga agcacgggct cgagaagatc aagacgatcg gcgacgcgta
     3301 catggtcgtc gcgggtatcc cgcagcccgt tgcgaaccac gcacacgcga tcgctcacat
     3361 ggcgctcgat atgctactcg ccgtcgacga tttcgcgaag cgacacgcgt cggacctcac
     3421 gattcgcatc ggcatccaca ccggccccgt ggtcgcgggc gtcatcgggc agaagaagtt
     3481 catctacgac ctgtggggcg acaccgtgaa caccgcgagc cgcatggagt cgcacggtgt
     3541 ccccggaaaa gttcacgtca ccgccaccac gttcgcggca ttacgcgaca cgttcgaatt
     3601 ggagccgcgc gaaccgatcg agatcaaagg gaagggccgc atgcagactt acttgatcgt
     3661 cggcaagcgt gcgacgatga agtcactcga gccgcgcccg agtgatccgc gcggctgggg
     3721 gtcggaaacg gacgggggat gacaagcaag cttcggtgga gtgcaccagc atgggcgctg
     3781 ttcacgacgc gtcacgcgac cgagacgctc gctgatgatc tgcccctcgc cgtcaccgtc
     3841 gagcagatgg gtccggcgcg catcctccac ctcggtatcg gcaagggcaa agtctcgatc
     3901 gcgatcgcgc tcggcaagaa accgaacgcg acgttcaccg tgcgcaccgc tgcggaggga
     3961 gccgcatgtg tcgacgcgct cgcgacgtgg ctcggtgtac ccgtgccacc gccctccacg
     4021 ggcgcggtcg caatgccgcc gccggtcacc gtcggtgtga tcgacatggg gcgcggcaag
     4081 ggccagcgcc acgtgtggca gctcgtgcag atcgtgctcg ggtacgaagc gcgcttctat
     4141 ttgatctggc gtgtcgccgg tacggaagcg tttctctgcg agcacgagtc tggcaaccgc
     4201 gaatcgttga tcgcagagct tgccgcggtg ctgcgcgatg gcacgcgtga ggtcagcgcg
     4261 gcacagccaa cgaacggcca ctacttcgtg ttcgacggcg aacggagcaa cgtcgacacg
     4321 cgtgcgtggg cgcgcaagct ccacctcgaa aaagcggacg tcgagcgccg cctcgaagca
     4381 gccggccgcg cgcacgtcga tccgctctac gcgcagatcg aaacggcgct cgacgcgtgg
     4441 cccgaagatc cgcgcggcgc tgccacgcgc gtcatgaaga tcccggtgta cggctgcgtc
     4501 ggcgcagtgt ggggctcgat cgcgcatcgc gatccggcga cgctgagcac gcaagaagtg
     4561 gccgcgctgc accaaaagct gcgatctctt gccgctgcgg actacgtgca gcggctctac
     4621 caccaggacg aggtgcatgc gctcctgcta aggtgagcgc gttcatgtgg tccgacgtcg
     4681 cggccattta tcggtcgtcg aagaaaaaga aagacatcaa ctggttcacc gagtggatcg
     4741 ctcgtccacc gggggccgtg gtcgtctact tcttgcgcaa cacgcgcgtc acgccgaacc
     4801 agatcacgtt cgcgtccgca ttcatcgcag cgggtgcgtg cgcgatgtt
//
LOCUS       DASUFX010000821         7356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2079674, whole genome shotgun sequence.
ACCESSION   DASUFX010000821 DASUFX010000000
VERSION     DASUFX010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079674"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFV99_33095"
     CDS             complement(<1..258)
                     /locus_tag="VFV99_33095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5064274.1"
                     /translation="MQSALPILTKAASGDIAVADPLGTFRLEGQLIDASDTPVGGGRV
                     TIDALPPRATTTEADGTFVFEGLLARDYTLAAIAEAGAAGPV"
     gene            complement(498..1307)
                     /locus_tag="VFV99_33100"
     CDS             complement(498..1307)
                     /locus_tag="VFV99_33100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5064275.1"
                     /translation="MLAAPPARASTATIVGVIIALAISVAVIWSVLFSWSGLDVVRHA
                     TAGLIQPAFVAALLVFALVGSVMYAGSVSVADAGLVASDLRRALVVLLVVASGIQIGL
                     VAGALISDGELHAGPALNDDPGTVAGNLIAHLLGTALVEEVAFRGFLLRQFLIRARAR
                     HADWRGVTLAVSAAALLFALYHIPQRMSLGLRGIDLVGSLAVVWVGGVLASYLYLRSG
                     SLGIVIVLHALFNYSAPLVASPVPEQWILGGLVLVVIGKIELDARRHEARS"
     gene            complement(1320..2540)
                     /locus_tag="VFV99_33105"
     CDS             complement(1320..2540)
                     /locus_tag="VFV99_33105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064276.1"
                     /translation="MRADAPISIVRALDGCELTRLDSAERGLSCAIITEDGRFLKLSG
                     SAALLVLGLHRGESLDELARQCDASVTDVLATCEALATKINSVRAAPRREALPGFVIT
                     REWLSSATVRAITAPVAALCGPRSMIALGALAVAILGFVLTGAPSPRLTLGSFWLAYL
                     ANLPILVVHELGHAATCARYGARVGPIGATIYMIYPALYCDVTDAWRLRRWERVAVDL
                     GGLYFQAIITAMIALGWYVTGSDVLYATAMMSVGIAITNLIPFFALDGYWCLSDALGV
                     VNLNHQRRVVLAGIWARVRGRVAPPFPWSAPLTAFVVAYSAAAMCFAGYVLWRLVPIA
                     GRHLAGLPQIIVQLVTASADADLMIALAALGRLATSMLVVVGAALLTRHVIRRSMTMI
                     ARRIRREQPSPPVS"
     gene            complement(2530..4104)
                     /locus_tag="VFV99_33110"
     CDS             complement(2530..4104)
                     /locus_tag="VFV99_33110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5064277.1"
                     /translation="MRTSSYTIYVDLPDSPDEQLLVHGYSGAYDVVERRVADYLRAHE
                     DDRHTPLHGEWPQESQRVTPRVPVHLPDETIEMLRRRGFLTARSAIEERELVKKAATR
                     RHAVERENRPTYILMPTYQCNLRCGYCFQDHMRTNPAFSHLLKTMSKDMAKRIVRAMD
                     KIDALHPNAAGKPRRITLFGGEPLLAENRPFIEWWINELKAAAPCEIAAVSNATQLEA
                     YADLLSPDIIDHVQITIDGPPDVHDQRRIHADGSGSFEMIAKNTTMALERGINIAMRV
                     NVDKFNLARLPELARAIIAHGWDKNPKFMAYAAAVHASNEQTDKKDTYNGYAVSLRML
                     ELREKHPELRVIARPSDELRDMVFQILRGHAQPWQMYKSAFCGAHSTMYVFDAFGDIY
                     ACWERTGDPSIRIGTIDEDGTPQLMRRRDPTTPAAEPAPVKRLLPVLGKPIVNDVDTW
                     RERTFATNDTCASCRYAFYCGGGCAAEAMDHRGEFHTNYCNGFQQRFRVAAADAYSDV
                     KQGRIGAFAQTVGCAS"
     gene            4396..4551
                     /locus_tag="VFV99_33115"
     CDS             4396..4551
                     /locus_tag="VFV99_33115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064278.1"
                     /translation="MTPEALDVEELEHRLELAAQTAACSCCGSNGNTCDCNTKVKAST
                     TTGGAGC"
     gene            4674..5519
                     /locus_tag="VFV99_33120"
     CDS             4674..5519
                     /locus_tag="VFV99_33120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064279.1"
                     /translation="MSKPTSCRWTAGLAPIDGAGNIYANVLEAFLAIMPPAAQAHLEV
                     AREAGRLLARSIVCADRVIDGDLTYGSFGENVLAAQICQLDAVRLLQRLVPADHEFWS
                     AFASRLRELADACLAELADTERDVAAAERIALGKNAVARLAASLACVLGGTAEPLAEL
                     DRAVVELIAAVQALDDAADWRADLARGQLSLVTARIWMQLGRDATGTAVDVFVYRVAL
                     PEVLHAGRTALENAMTMPALRGSAAWTRMTGKIAHAYDHAIAVLPFREARPDRSSGAI
                     EEAAQ"
     gene            5528..6412
                     /locus_tag="VFV99_33125"
     CDS             5528..6412
                     /locus_tag="VFV99_33125"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5064280.1"
                     /translation="MSWLNDTSEDSDPVERRMREVLTQRDPIAFAPVHEIALSKSKRL
                     RSGLAQIIARAIDLPDRVAVDIAVAIEIVHTFTLVHDDLEDGATTRRGCSAVHVAHGA
                     PIAINVGDALHALAWSIITALDEPPQRTLQVAKLFGVTLDRVVAGQARDLVWTRDRPS
                     GLHLEQYIEMVRGKSGALLGFSAAAPAVAHGHAGADRLYTFGEELGIALQLLDDVAGV
                     QGDASILGKPVGADANGFASAPMLVGVARSIELAARHVDRALEELYAANVPRPREARL
                     FTKAMHDCLLERAGAKES"
     gene            6421..>7356
                     /locus_tag="VFV99_33130"
     CDS             6421..>7356
                     /locus_tag="VFV99_33130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064281.1"
                     /translation="MQLPFDQSQLAAIIACDHAFERLSASWRDGVDWRPTDRRDIRRI
                     YEMYPMLFAPAFPNISEDALRTLVVACRLLSEALFAFDEVIDEALASRERATGIAKGQ
                     SLQLDAICTFAELFPPTSEFWPALHACFLAYVAAIQKEREIALADLDEATAVELGRGK
                     TAVARSCIAAMGIVGSRPDVAAVIADAVSEYYVARQMWDDLADWRKDLASGQHSLLLA
                     RAIASLAPDSARDAQSVGVAVYYGGHADHVLAIGSAALERAHASIASLGVALPFGRLL
                     DDLAKQFRETRAGLTDLVERKRRAPGARPHALPAQP"
BASE COUNT         1264 a         2439 c         2446 g         1207 t
ORIGIN      
        1 gacgggcccc gcagcgccgg cttctgcgat cgcggcgagc gtgtagtcgc gagcgagcaa
       61 accttcgaac acgaacgtgc cgtccgcctc cgtcgtcgtt gcgcgcggtg gtagggcgtc
      121 gatcgtcacg cgcccaccgc ccacgggtgt atcgctggcg tcgatgagct ggccttcgag
      181 gcggaatgtt ccgagcggat cagcgaccgc aatgtcaccg ctcgctgctt tcgtgaggat
      241 cggtagagcc gattgcacgg cgcggggtga aggcgcggct gcatcgccgt gacgggtgcg
      301 gcttgccgac cccgccgcga tcacgcccac caccgtgacg agcacggcaa gacctacgat
      361 ttttgaggtt cgcgtcatgc gccgccccaa agcaagtgag atgccggcgc tcgagcggct
      421 atgtccgcac aacacgatga cgacgcgagc gatccgttac acgtaccgct gttccgtcgt
      481 tgctccggct gttccgctca cgagcgagct tcgtgccggc gcgcgtcgag ctcgatcttg
      541 ccgatcacga cgagcacgag cccgccgagg atccactgct ccggcactgg tgacgccacg
      601 agtggcgccg agtagttgaa cagagcgtgc aggacgatca cgatgccgag gctaccgctg
      661 cgcaggtaca gatagctcgc gagcacaccg ccgacccaga cgaccgcgag gctgccgacg
      721 agatcgatgc cccgcaagcc aaggctcatc cgttgtggga tgtggtagag cgcgaacagc
      781 aacgccgccg ccgagaccgc gagtgttacg ccgcgccagt ctgcatgacg agcgcgcgcg
      841 cggatcaaga actgacgcaa caggaagcca cgaaacgcga cctcttcgac gagcgcagtt
      901 ccgagcaggt gcgcgatcaa gttgccggcg acggtgcccg gatcatcgtt cagcgcgggt
      961 ccggcgtgga gctcgccgtc ggagatgagc gcacccgcga cgagcccgat ctggatgccg
     1021 ctcgcaacga cgagcagaac gacgagcgcg cgccgcaggt cgctggcgac aagccctgca
     1081 tccgcgaccg agaccgaacc ggcgtacatg acgctgccga ccagcgcgaa gacgaggagc
     1141 gcggcgacaa acgcgggctg gatcaggcca gcggtcgcgt gacgcacgac atcgagaccc
     1201 gaccagctga acagcacgga ccagatcacg gcgaccgaga tcgccagcgc gatgatcacg
     1261 ccgacgatcg tcgcggtgct tgcacgcgct ggtggtgccg cgagcatggg cctcacgtat
     1321 cacgataccg gtggcgacgg ttgttcgcgg cggatcctgc gcgcgatcat cgtcatgctc
     1381 ctgcggatga cgtggcgagt gagcagcgcc gcgcccacca ccacgagcat cgacgtggcg
     1441 agccggccga gcgcagccag cgcgatcatc agatcggcgt ccgcgctcgc ggtgacgagc
     1501 tgaacaatga tctgcggcaa cccggccagg tgccggccgg cgatcggaac cagcctccac
     1561 agcacgtagc ctgcgaagca catcgcggcc gccgagtagg cgacgacaaa tgcggtcagg
     1621 ggcgccgacc acgggaatgg aggagcgacg cgaccccgca cccgcgccca gatcccggca
     1681 agcacgaccc tgcgttggtg attgaggttg acgacgccga gcgcatcgga caggcaccag
     1741 tagccatcga gcgcgaagaa cgggatcaag ttcgtgatcg cgatgcccac gctcatcatc
     1801 gcggtggcgt acagcacgtc cgagccggtg acgtaccagc cgagcgcgat catcgccgtg
     1861 atgattgcct ggaaatagag accgccgaga tcgacggcga cccgctccca acggcgtagt
     1921 cgccacgcat cggtgacatc gcaatacagg gctggataga tcatgtagat cgtggcgccg
     1981 atcggaccga cgcgtgcgcc atagcgagcg cacgtggccg cgtgaccaag ctcgtggacg
     2041 acgaggatcg gcaggtttgc gagataggcg agccagaacg atccgagcgt cagtcgtggt
     2101 gacggagcgc cggtgagcac gaagccgagg atcgcgaccg cgagcgcgcc gagcgcgatc
     2161 atcgagcgtg gtccgcacag ggcagcgacg ggcgcggtga tcgcgcggac ggtcgcgctc
     2221 gacaaccact cgcgtgtgat cacgaagccg ggcagcgctt cgcgcctcgg cgcggcccgc
     2281 acggaattga tcttcgtcgc gagcgcttca cacgtggcca ggacatcggt caccgacgcg
     2341 tcacactgcc gagcgagctc gtctagcgac tcgccgcggt ggaggccgag cacgagcagc
     2401 gccgccgatc cggatagctt gaggaacctg ccgtcctcgg tgatgatcgc gcacgacaga
     2461 ccacgctcgg cggagtcgag gcgagttagc tcgcagccgt cgagggcgcg gacgatcgag
     2521 atcggtgcgt cagctcgcac agcccacggt ctgggcgaac gcgccgatgc ggccctgctt
     2581 tacatccgag taggcgtccg ccgcggcgac acggaaccgc tgctggaatc cgttgcagta
     2641 gttcgtatgg aactcgccgc gatggtccat tgcctccgcc gcgcagccgc cgccgcagta
     2701 gaacgcgtag cggcagcttg cacatgtatc gttggttgcg aacgtgcgct cgcgccaggt
     2761 gtcgacgtcg ttgacgattg gcttgccgag aacggggagg agtcgcttga ccggcgccgg
     2821 ctcggccgca ggagtcgtgg gatcccggcg gcgcatcaac tgcggagtac catcctcgtc
     2881 gatcgtaccg atgcggatcg acggatcgcc ggtgcgctcc cagcacgcgt agatatcgcc
     2941 gaacgcatcg aacacgtaca tcgtgctgtg cgcgccgcag aatgctgatt tgtacatctg
     3001 ccacggctgc gcatggccgc gcaggatctg gaacaccatg tcgcggagct cgtcgctcgg
     3061 cctcgcgatt acgcgcagct cggggtgttt ctcgcgcagc tcgagcatgc gcaggctgac
     3121 cgcgtacccg ttgtaggtgt ccttcttgtc agtctgctcg ttcgaggcgt gcacggcggc
     3181 ggcgtacgcc atgaacttcg ggttcttgtc ccagccgtgc gcgatgatcg cgcgcgcgag
     3241 ctcgggtagg cgtgcgaggt tgaacttgtc gacgttgaca cgcatcgcga tgttgatgcc
     3301 gcgctcgagc gccatcgtcg tgttcttcgc gatcatctcg aacgagcccg agccatcggc
     3361 atggattcgc ctctgatcgt ggacgtcggg cgggccgtcg atcgtgatct ggacgtggtc
     3421 gatgatgtcg gggctcaaca gatccgcata cgcctcgagc tgcgtcgcgt tcgacaccgc
     3481 agcgatctca caaggggccg ccgccttgag ctcgttgatc caccactcga tgaacggtcg
     3541 gttctcggcc aacagtggct caccaccgaa cagggtaatg cggcgcggct tgcccgcggc
     3601 gttgggatga agcgcgtcga tcttgtccat cgcacgcacg atccgcttcg ccatgtcctt
     3661 cgacatcgtc ttgagcagat gcgagaacgc ggggttcgtg cgcatgtgat cctggaagca
     3721 gtagccacag cgcaggttgc actgataggt cggcatcagg atgtaggttg ggcggttctc
     3781 gcgctcgaca gcgtgtcgac gcgtcgcggc cttcttcacc agctcgcgtt cctcgatcgc
     3841 cgatcgcgcg gtcagaaagc cgcggcgacg caacatctcg atcgtctcgt ctggaaggtg
     3901 gaccggcacg cgtggcgtta ctcgctgcga ctcttgcggc cactcgccgt gcagtggtgt
     3961 gtggcgatcg tcctcgtggg cgcgcaggta atcggccacc cgacgctcga cgacgtcata
     4021 cgccccgcta tatccgtgca cgagtagctg ctcgtccgga gagtcgggaa gatcgacata
     4081 tatggtgtag ctgctcgttc gcatggtcga gcgcggcttg cagcggaagt gccagcttgc
     4141 tcgccggtaa ctgcacgaga cgcgcgcgtt gcgcgctcga atggtgattc gatcgttcca
     4201 cccagtgttc cgatcgcggc tgagaagctg gctcggacgc cgacaagcct gtggcgttcg
     4261 gttcgttcgc gcgtcctcgc tgctggaatg acgcgtgcaa tgcccacgcg gcggcatgtc
     4321 cgatctcttt cgagacgatc gaatttcatc atcaacaagg acaaatcaat gagcgaaaaa
     4381 ccacgcactc ttcacatgac ccctgaagca cttgacgtcg aggagctcga gcaccgactc
     4441 gagctcgcgg cgcagacggc tgcctgcagc tgctgcggct cgaacggcaa cacctgtgac
     4501 tgcaacacca aggtcaaggc ttcgaccact actggcggag ctggctgtta acctgactta
     4561 gttcgttcga gctagacgcg gacatgcctc ttcgtttcta gccgcatcgc gatctggatc
     4621 gcgacgacag gcgagctcgc tcgcctgccg ggcccttttt cttcgaggtc gacatgagca
     4681 aacccacgag ctgccggtgg accgccggcc tcgcgccgat cgacggcgcg ggcaatatct
     4741 atgcaaacgt tctcgaggcg ttcctcgcga tcatgccgcc ggccgcgcaa gcgcacctcg
     4801 aagtagcgcg cgaggcgggg cggttactcg cgcgctcgat cgtctgcgcc gaccgcgtca
     4861 tcgacggcga cctcacctac ggcagcttcg gcgagaacgt ccttgccgcg cagatctgcc
     4921 agctcgacgc tgtgcgccta ttgcagcggc tggttccggc ggatcacgag ttctggtccg
     4981 cgttcgcgag ccggctccgc gagctcgccg acgcgtgcct ggcggagctc gcagacacgg
     5041 agcgcgatgt cgccgcggcc gagcgcatcg ctctcggcaa gaacgcggtc gcccgactcg
     5101 ccgcctcgct cgcgtgtgtg ctcggtggca cggccgagcc gctcgcggag ctcgaccgtg
     5161 cagtcgtcga gctgattgcc gccgtgcagg ccctcgacga tgcggccgac tggcgcgccg
     5221 acctcgcgcg cggccagcta tcgctcgtca ccgcgcgcat ctggatgcag ctcggccgcg
     5281 atgcgaccgg aaccgcggtc gatgtcttcg tgtaccgcgt cgcgctgccc gaggttctcc
     5341 atgccgggcg cactgcgctc gagaatgcga tgacgatgcc tgcgctccgg ggctccgcag
     5401 cctggacccg gatgacaggc aagatcgcac acgcctacga tcacgcgatc gcggtgcttc
     5461 cgttccgcga agcacggcca gaccgttcgt caggagcgat cgaggaggca gcgcaatgat
     5521 cgctgcggtg agctggctga acgacaccag cgaggactct gatccggtcg agcgccgcat
     5581 gcgcgaggtg ctgacccagc gcgatcccat cgcattcgca ccggttcacg agatcgcgct
     5641 ttcgaagagc aagcggctgc gttccgggct cgcgcagatc atcgcgcgcg ccatcgatct
     5701 gccggaccgt gtagcagtcg atatcgcggt cgccatcgag atcgtgcaca cgttcacgct
     5761 cgtgcacgac gacctggaag acggcgcgac gacgcgtcgt ggctgttcgg ccgtgcacgt
     5821 cgcccacggt gctccgatcg cgatcaacgt cggcgatgca ctgcatgcac tggcctggtc
     5881 gatcatcacg gcgctcgacg agccgccgca acgtacgctg caggtcgcga agctgttcgg
     5941 cgtcacgctc gatcgcgtcg ttgccgggca ggcacgcgac ctcgtgtgga cgcgcgatcg
     6001 tcccagcggc ctgcatctcg agcagtacat cgagatggtg cgcggcaagt cgggtgcgct
     6061 gctgggcttc tccgccgcgg ccccagcagt ggcacacggc cacgccggcg cggaccggct
     6121 ctacacgttt ggcgaggagc tcggcatcgc gctgcaactc ctcgacgacg tcgccggcgt
     6181 gcaaggcgat gcctcgatcc tcggcaaacc cgtcggtgcc gatgccaacg gattcgcatc
     6241 ggcacccatg ctcgtcggcg tagcacgcag catcgagctc gcagcgcgtc acgtcgaccg
     6301 tgcgttggag gagctttacg ccgcgaacgt tccgagaccg cgcgaagcgc gactgtttac
     6361 caaggccatg cacgactgcc tgctcgagcg cgcgggcgcg aaggaatcgt gaaaggatcc
     6421 atgcagcttc cattcgatca gagccagctc gccgccatca ttgcctgcga tcacgcattc
     6481 gagcggctca gcgcgagctg gcgcgacggc gtcgactggc gaccgaccga ccggcgcgac
     6541 atccgccgga tctacgagat gtatccgatg ctgttcgcgc cggcattccc caacatttcg
     6601 gaggatgcgt tgcggacgct cgtcgtcgcg tgccgactgc tttccgaagc gctgttcgcg
     6661 ttcgacgagg tgatcgacga agcgctcgct tctcgtgagc gcgcgacggg catcgccaag
     6721 ggccagagcc tccagctcga tgcgatctgt acgttcgccg agttgttccc acccacgagc
     6781 gagttctggc cggctctcca cgcttgcttc ctcgcctacg tggcggcgat ccagaaggag
     6841 cgcgagatcg cactcgccga cctcgacgag gcaacagcgg tcgagctcgg gcgtggcaag
     6901 acggcagtcg cgcgcagctg catcgccgcg atggggatcg tcggcagtcg tcctgacgtc
     6961 gccgccgtga tcgcggatgc cgtgagcgag tactacgtcg cgcgccagat gtgggacgac
     7021 ctcgcggact ggcgcaagga tctcgcgagc ggccagcact cgttgctact cgcgcgcgcg
     7081 attgccagcc ttgcccccga tagtgcgcgc gacgcgcaga gcgtcggcgt tgcggtgtac
     7141 tacggaggtc acgccgacca cgtgctcgcg atcggcagtg ccgcgctcga gcgggcgcac
     7201 gccagtatcg cgtcgctcgg cgtcgcgttg ccgttcggcc ggctgctcga cgacctcgcg
     7261 aagcagttcc gcgaaacccg cgccggactg accgacctcg tcgagcgcaa gcggcgcgcg
     7321 cccggtgcgc ggccccacgc cctgccggcc cagcca
//
LOCUS       DASUFX010000822         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_132672,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000822 DASUFX010000000
VERSION     DASUFX010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132672"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFV99_33135"
     CDS             <1..883
                     /locus_tag="VFV99_33135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064282.1"
                     /translation="LAILIVVITRVPFDAFGDAIEHGPHVKLAIIELLITFSVLCTDS
                     IATWLGLIAVRLRRPFLTVMAVRGATYALFVINYAVGQGAFGYYLAKAGVRPLRATGA
                     TLFLVGTNLATMLVLLSLVWALGDLAVPTARLTWTLTLGCSAFAAYLLVIALAPHFLA
                     RRELFAPLFDAGLRGHGLAMLGRVPHVFVLVLAQWIALRTWGIQVPFVVGVLILPIIV
                     IAAALPISPAGLGTAQAAFVYFFQGFAPGLNVDDRSAHVVAFGIVHFVYGAAASLLVG
                     LVCVPIAKRRGALREGT"
     gene            936..2234
                     /locus_tag="VFV99_33140"
     CDS             936..2234
                     /locus_tag="VFV99_33140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064283.1"
                     /translation="MRWTSELSPRTWLLVGWVIFGLCSYPGYLSIESGLQLYNVRTGV
                     YSDAFSPVMTGLWSLLEVVFAGPAPMLALQSGLFLFGLAAILRTILSPRAAALTACGV
                     LLFPPVFAVMAVIWPDPLLASALVAGTGALLQHRRAWRITGGILLAFACACRPEVTVA
                     LAPIVFLFVPKGVWSKRVGLTIAVVVGLSSVARITEWVLTDDVSLEWQQQLLLPDVVS
                     TIRKGHPNPATLDRLVAGLPKAATPDADDRLVRGRDALDVWTLTHGEKRVFEPIATEE
                     QAEAMRAAWRHAIAQYPLGYLKHRRVMTWHLLGISGKWAPVYDDFGDPELLAPLHHRA
                     IPSDWQLGWQIVVRGVSKTPLFRPWLYVVLAIVLVYFTRKQRVLRALVVSGILYELTW
                     MFFAPSPDYRLSHWLVTTTTIAGIALLVGRRWPSPDLRPE"
     gene            complement(2211..>2596)
                     /locus_tag="VFV99_33145"
     CDS             complement(2211..>2596)
                     /locus_tag="VFV99_33145"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX5064284.1"
                     /translation="VGYAVAIPVTVLSPWIITGLFGAAYERAAPMLTVLVWASVVTNL
                     GIARSGFLTAMNWTRLHFASSVLAALANVLLNLWLIPRYGGVGAAIASLIAYWIGSQG
                     TCLLFKPLRRTAWMMTKALLWPKIW"
BASE COUNT          382 a          860 c          843 g          511 t
ORIGIN      
        1 gctcgcgatt cttatcgtcg tgatcacgcg cgtgccgttc gacgcgttcg gggatgcgat
       61 cgagcatggc ccgcacgtga agctcgcgat catcgagctg ttgatcacgt tctcggtgct
      121 gtgcaccgat tcgatcgcaa catggctggg cctgatcgcg gtgcgcctac ggcggccgtt
      181 cctcacagtg atggccgtgc gcggcgcgac ctacgcgctg ttcgtgatca actacgccgt
      241 tggtcaaggc gcgttcggct actacctcgc aaaagccggt gtcaggccgc tgcgcgcgac
      301 cggtgcgacg ctgtttctcg tcggaaccaa cttggccacc atgctcgtgc tcttgtcgct
      361 cgtgtgggcg ctcggtgacc tcgcagtgcc gacggcgcgg ttgacctgga cactgacctt
      421 gggctgttcc gcgttcgccg catacctcct cgtgatcgca ctcgcgccac atttcctcgc
      481 ccgccgcgag ttattcgcac cgttgttcga tgccgggctt cgtggccacg ggctcgcgat
      541 gctcggacgc gtgccccacg tgttcgtgct cgtgctcgcg caatggatcg cgctgcgaac
      601 ctggggcatc caggtgccgt tcgtcgtcgg cgtactgatc cttccgatca tcgtgatcgc
      661 ggcggcgctg ccgatttcgc cggccgggct cggcaccgct caggcggcgt tcgtctactt
      721 cttccagggg ttcgcgcccg gcctcaacgt cgatgaccgg tcggcgcacg tcgtggcatt
      781 cggcatcgtt cacttcgtgt acggcgctgc ggcgtcgctg ctcgtcggtc tcgtttgcgt
      841 tccgatcgcg aagcggcgcg gcgcactacg cgaaggcacg tgaatcgtgg tatcgcgcta
      901 gcgacatgga catcggcgcc agctggcgaa gagtcatgcg ctggacgagc gagctctcgc
      961 cacgcacgtg gctacttgtc ggttgggtga tcttcggcct ctgctcgtac cccgggtact
     1021 tgagcatcga gtcgggcttg cagctgtaca acgtccggac cggcgtctat tcggatgcct
     1081 tctcgccggt gatgaccggc ctgtggagcc tgctcgaggt cgtattcgcc gggcccgctc
     1141 cgatgctcgc gctgcagagc ggcctgttcc tgttcgggct cgctgcgatt ctccgcacga
     1201 tcttgtcgcc ccgcgcagcg gcgctcactg cgtgcggcgt actgctgttc ccacccgtgt
     1261 tcgccgtgat ggccgtgatc tggccagacc cactgctcgc gagcgcgctc gttgcgggca
     1321 ccggtgcgct gctccagcat cgacgcgcat ggcgaatcac gggcggcata ctgctcgcgt
     1381 tcgcgtgtgc gtgccgaccg gaagtcacgg tcgcgctcgc gccaatcgtg ttcttgttcg
     1441 tgccgaaggg cgtgtggtcg aaacgcgtcg ggctgacgat cgcggtcgtc gtcggtctca
     1501 gcagcgtcgc acgcatcacc gagtgggtgc tcaccgacga cgtttcgctc gagtggcagc
     1561 agcagctgtt actgccggat gtcgtcagca cgatccgcaa aggccatccg aatccagcga
     1621 cgctcgaccg gctcgtcgcc gggctgccga aggccgcaac cccagacgcc gacgaccgac
     1681 tggtgcgcgg ccgtgatgcg ctcgatgtct ggacgttgac gcatggcgag aagcgcgtgt
     1741 tcgagccgat cgcgaccgag gagcaagcgg aagcgatgcg cgcggcgtgg cgccatgcga
     1801 ttgcccagta cccactcggt tatctcaagc accgccgcgt gatgacctgg cacctgctcg
     1861 gcatctcggg caagtgggcg ccggtctacg atgacttcgg cgatcccgag ctactcgcac
     1921 cgctgcacca ccgcgcgatt ccctcggact ggcagctcgg ctggcagatc gtcgtgcgcg
     1981 gggttagcaa gacgccattg ttccggccgt ggctctatgt ggtcctcgcg atcgtgctcg
     2041 tgtacttcac gcgcaagcag cgcgtgctgc gcgcgctggt cgtgagcggc atcctatacg
     2101 agctgacatg gatgttcttc gcgccgagcc ccgactaccg cttgtcgcac tggctcgtga
     2161 caacgacgac gattgcaggc atcgcgttgc tcgtcggtcg gcggtggccg tcaccagatc
     2221 ttcggccaga gtagcgcctt cgtcatcatc cacgccgtcc gtcgcagcgg cttgaacaac
     2281 aggcacgtgc cctggctgcc gatccagtag gcgatcagcg aggcgatcgc ggcaccgacg
     2341 ccgccatagc gcgggatcag ccacaggttg agcagcacgt tcgcaagcgc ggcaagcacc
     2401 gacgacgcga agtgcaagcg cgtccagttc atcgcggtca gaaatccaga gcgtgcgatg
     2461 ccgagattcg tcacgacact cgcccaaacc agcaccgtga gcatgggtgc cgcacgttcg
     2521 tacgctgcac cgaacaagcc ggtgatgatc cacggcgaga gcacggtgac cgggatcgcg
     2581 actgcgtagc ctacca
//
LOCUS       DASUFX010000823        19198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1245792, whole genome shotgun sequence.
ACCESSION   DASUFX010000823 DASUFX010000000
VERSION     DASUFX010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19198
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245792"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2272
                     /locus_tag="VFV99_33150"
     CDS             <1..2272
                     /locus_tag="VFV99_33150"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064285.1"
                     /translation="IRGVVADVVRAPELLEVAVEAALGDRLGGVLVSEPEVGVAAIGF
                     LKQGGGGRSAFVPLETRSAVPVLVGPTASTPVPFEGEGGGSSWMSPAWAADDDSPVKQ
                     TIGSGPTWLNPDWSDGNPFAHNESGNSGKIEVEDRSELVAASIAIAKSPELAGEGVLG
                     RMADLVSFADGYEDVGKRLLAGTVVVDQLDRAIRLHEQGVKDRLVTLDGDVVDEDGVV
                     AGGSRDAQGAGVLAQKREIRDLEEIVGKLEHDLSEAMARLVTAKTELKQVMKALDGLR
                     TQVHEGDVAIMGHEKDESRVRHELERHRDRLGQLGNEQLELEHRIRAIEIDDAAARER
                     KTQAIERIAELERAQLDLISEVSSHRDQLEQLSASLTEARVRAAQLGEKRAAAEASAL
                     RLQTTDNELAARADRLTQEIDEAARRAVDLRAGCDALALELEEVRTRREAQVTELDQG
                     RVAYDQRLQALTEVELTARELRSQADKLAQELQQLELRLGQIKMTKQVVHDGVDERYQ
                     IEITDVLFDYHQRSPITEVEDARLAELKDLIDRMGTDINLTAIEEFSEVSTRFEFLSA
                     QKIDLERAVDQLQRAIDKINKTSRKLFKDTFAAVNATFKEVFPRLFRGGQAALSLSGG
                     EGVDLLEAGVEIMAQPPGKKNSTVDQLSGGEKALTAVALVFSIFLIKPSPFCILDEVD
                     APLDEANVDRYNEIVREMTDRSQFIVITHNKRTMESADKLYGVTRQEPGVSKLVSVNL
                     AKLGAQKANSPQSVAV"
     gene            2826..3842
                     /locus_tag="VFV99_33155"
     CDS             2826..3842
                     /locus_tag="VFV99_33155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064286.1"
                     /translation="MRFARWQIAVALVAGCYAPNANIGAPCPDGLCPSGQMCDVDHVG
                     GPTCVLPGTGGSGSGSSEVDSGGGETFYDAAPEDTMTIMMPDAPDLPRPANDSPANPI
                     DVSAGGMFMWDPTNALDDVASPCAASTGPDVFFTITLAAPEVIYFDTFGSSADSVIAI
                     YPGDCTPLGTIEACVDNSCGGTKSQGAWNLTAGTHCIVVDQVGSTGGTTGKLNVVHGR
                     RAGDPLVGASGTVSGDTCNDDNSNNASCGSEPSLDHHYFVTVCPNSAPNLHIETCNRA
                     NWDTVLQIRSNATSGSTLQCGDDSCDNTRTSIDATLTGPGLFWAIIDGYDDCGQYTMA
                     FTLQ"
     gene            3976..5160
                     /locus_tag="VFV99_33160"
     CDS             3976..5160
                     /locus_tag="VFV99_33160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5064287.1"
                     /translation="MAEPTQPDRETREQFLGVLAELIAQGGAERFLLPPVAPGGDAFP
                     EPWAPTTAGVQLLLRRLLWHAGIERAVELDDDRLAKAPPTERKPATRVEITELAKTTP
                     AGSKAAGSARFTVYFIGEDEIAGTLAHEVGTLYAALHRVEKADPYRTAEPPTITIDPD
                     RDLERGSIATVYLGLGVLAANAAFQQYSSAGRFNGGYVPLEYDVLRAGAVPMSALAYL
                     LAVQATVRGDDAPPRGLAPPQRDEVADWMKALDGQGATLRERLGIAASTAVQARPAVE
                     PLDDELPPFEEPAPKPTAFRWQTHRGGVGFVAGTVMGVGLAFALARGMLPLTLVGGGT
                     LGHLIGRRVRAPRCSACATVLPEGATTCAKCGAVLRGDIANLGDRLEAEERLEEENRE
                     QL"
     gene            5374..5568
                     /locus_tag="VFV99_33165"
     CDS             5374..5568
                     /locus_tag="VFV99_33165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064288.1"
                     /translation="MGNLNEIKERHGRDRLKDAGFIAAALLLTALAIGSVTSKAAGKP
                     HTHEWKLSVVDPVSNVEIGR"
     gene            complement(5893..7563)
                     /locus_tag="VFV99_33170"
     CDS             complement(5893..7563)
                     /locus_tag="VFV99_33170"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5064289.1"
                     /translation="MIPRLATRGKLVLGTALLFLIVGALHGAPPLVAMAGAILSVLLA
                     LYLMFYPTAILLRRKKIELSWWVPPGDQFGGALAVDRPFQLHLAFRNHGSRRLRVLST
                     NILGASGLGIDERPSATVHRGQQVEVTTTVKPLTAGYHVLHGAALTFGDALGLFDIEA
                     FFPNPIAVKVFPRTMALRGQITRAVGGALHEQVGLHHVRRRGLSGELRELREHTHGDP
                     FKFIAWKATARRQQLMVRDLENEIVTTHVILVDVGAGMRQGPLGRSPIDWACDATTSL
                     AKAAIGNSDRVGLVGFDTRPLVELAPDTGHHHYLQVIDRMLDIRSVVDADLTDVTAGE
                     LVALVARYLAHQEAIDVRIKVAPPLDDQRWMQIQAGPDGQLYDVAATGRLCKRLIDVM
                     LGKEGQKKMKPPHVAEDDVQLRPLRQFCRLRGIELPYRTTWEHGRRAHGFAAAVERAV
                     ANGRPDVVVLISDLAGFAEDDVRVKRALARLRKSAGSVIAIAPSANAFLPQATTPNGR
                     RIRELIVRDQRAALEPGRRLLVKHGITVLEGSPIVSLDQLLNGRRVAA"
     gene            complement(7694..8662)
                     /locus_tag="VFV99_33175"
     CDS             complement(7694..8662)
                     /locus_tag="VFV99_33175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5064290.1"
                     /translation="MQAALTHDKLSRVNQLARAITEEVGRAFIGSPTITEALLTALLA
                     RGHVLIEGYPGVAKTTLVKAFSATLGCGFRRIQFTPDLLPSDITGTYILDMRTNQFVL
                     REGPVFTNVLLGDEINRAPAKTQSALLEAMQEQQVTIEGETRSLSEPFIVLATQNPIE
                     QEGTYPLPEAQVDRFLIKLKMGYPVQADEKRMLHAYDRMPPPVRAVVGPNDVLEMQAL
                     AQNVFVAEELVDYVLGLVAFTRNHARVYLGASPRAGLALVHASKGLALLRGRDYVLPD
                     DVRHLAPLVLGHRILMTPEAELEGAAGTAVVAESLDKVGYKMPKRQ"
     gene            complement(8662..9981)
                     /locus_tag="VFV99_33180"
     CDS             complement(8662..9981)
                     /locus_tag="VFV99_33180"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX5064291.1"
                     /translation="MRSLVAVALALVATLAGTARAQVDDIPDYDPRSPAWNGMAVFVG
                     LAEGMGYEVTAVSTLEWGELSANDILFMVYPLEHVEPSRVDAFVQAGGNVVIADDFGE
                     GKEAMSRLGLLRAEVTTPKASKYQDGRVWAPIASVQADHPITRGVDEVVTNHPAALRE
                     VEGATTLVGFEDGAVVVAGERGTGRFVAVSDPSIFINRMLQYPGNVTLAANMLSWLDR
                     HETGNRARRVVLLRGKVPMYGEPRPFINDPRGGKLGRSIADLNTWLSQRREWLLTPTA
                     MKALAATLAALLLLLAVLALPVRRGPRIDGAWLRFGRPARRDEPFALVAASERNAGSA
                     LVLGCILRDQVQMLLADALGKPEPLYTLSEAQLVKQVSDARGRGAGDALARVYKRLRA
                     LPSRGQAAAPWSSGHLARRDFDALYRDVAELCRTLGAPLTEAAPEAA"
     gene            complement(9978..10343)
                     /locus_tag="VFV99_33185"
     CDS             complement(9978..10343)
                     /locus_tag="VFV99_33185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064292.1"
                     /translation="MTPFAQILQRAVQATPNAIGGAFADADGEMVDSYCIGDPHEWAV
                     LTAHYGVVMAHLNAAFGTWHFGGPEYFIAQHRELEIVVVAVHAGYYALLALTEPANLG
                     YALQQLRTASVELRREMGL"
     gene            complement(10340..11239)
                     /locus_tag="VFV99_33190"
     CDS             complement(10340..11239)
                     /locus_tag="VFV99_33190"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5064293.1"
                     /translation="MRRLVAIPMVLAACSGSHTSPPPTRSGPPAPMIATTHDDVQVAT
                     VEGRPVWGSCVTAQAARGATRDEALKQCIDFELMAIEAEKRGLTTDRDVVLATRTALV
                     SDLVAREYEDKYNRPEEFGTYWSRSVDKNRGRFDHPEARGSVYVRIDVPKQATPEQDA
                     AAKQIADDVYAALKDERGLMAPHVQEIATRVIGTRAPLDIKTVQASLQHGQLDETYTG
                     ALFAIPEIGRVAPPVRTPWGWDVILLDSIVPAVHLSGEQLVTEALPDVKRSYFTHWVD
                     KIASGLGLHAEIIDKNLPVLEKL"
     gene            complement(11243..11431)
                     /locus_tag="VFV99_33195"
     CDS             complement(11243..11431)
                     /locus_tag="VFV99_33195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064294.1"
                     /translation="MRRYFFEILAIALIAGSLFFFKETLDYLARRDYVAALLVMVIGV
                     AVISVGKEMARLALVQRD"
     gene            complement(11439..12218)
                     /locus_tag="VFV99_33200"
     CDS             complement(11439..12218)
                     /locus_tag="VFV99_33200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064295.1"
                     /translation="MTEQVTERKWRTFADVVAFTLGVNVWITIVILPAVFVAALRGKM
                     ILAAMLPFGMLMLGLARRSELILLGLFPSALLVPISLNPALASSHVYGPIRFVLVVLG
                     VIAYLFGVSFFTTFHEPPAPKSIRPLSSAAVGPAPRWQRRERVYWMLTVMSLLIPTVL
                     IAWVNFDQSIQDFLGEMYPGRVALMTTALTVGAIVLWLGIYHYAFLGALRPHRTGDRD
                     LVGALAQARADAKAGKPRGRFYLSVALALGAMLLLILLRHL"
     gene            12261..12995
                     /gene="cmk"
                     /locus_tag="VFV99_33205"
     CDS             12261..12995
                     /gene="cmk"
                     /locus_tag="VFV99_33205"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5064296.1"
                     /translation="MDSVVGPLGPAKVEVYTATMRRIVVTIDGPAGAGKSTVAKQLAR
                     RLGYRLLDTGAIYRAVALVAKRSGVPWTDAAACAAVARDLDISYQFVGDKNHVFVSGE
                     DVSTAIRTPDISQGASQVSAHPPVRAALMDIQRRVAEGGGLVVEGRDTGTVVFPKAEA
                     KFFLTATVEERARRRIAELEATGATVDHEQTLREIRERDERDASRDVAPMRPADDAVL
                     VDSSTQTIEQVVENLARVVEARADAR"
     gene            13130..14854
                     /locus_tag="VFV99_33210"
     CDS             13130..14854
                     /locus_tag="VFV99_33210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5064297.1"
                     /translation="MVSQEGQGDEDFAALLAESFGQENVKEGEILRGTVIAVGKDFAI
                     VDVGYKSEGQVPLEEFRMADGSIGVKPGDVVDVLLESRENDMGMVVLSKEKADRFKVW
                     DEISAACERDELIEGIITARVKGGLSVTIRGGVKAFLPGSQVDLRPVRNLDAFLGQNF
                     KFKVIKFNKKRGNIVLSRRVLLEKERAALKESTLERLKEGQLVEGIVKNLTEYGAFID
                     LGGIDGLLHITDMSWGRVNHPSELFQVGDHVRVRVLKFNADTERVSLGLKQISEDPWS
                     RAAEKYVPGTVVRGKVVSLKDYGAFIELEEGIEGLVHISEMSWTRRVKHPSKMVAVGD
                     MVEAVVLDVDVKQNRISLGMKQLEPNPYEQLTEKYPPGTVVKGHVRNIADFGIFVEIE
                     EGIDGLVHISDMSWTQRVKHPSELFQKGDEVEAVLLNIDTGDGDKPKISLGIKQLVQD
                     PWDRIPQEYPAGKVVDGKILKVLDFGAFVELEKGIEGLVHVSEISEEHVEDPRTVLQP
                     GQAVKVQVLHADSAERKIGLSIKGANRAKELADAQGYASPNAGGATLGDKFRGKLDKL
                     KSGAKDAQ"
     gene            14981..15619
                     /locus_tag="VFV99_33215"
     CDS             14981..15619
                     /locus_tag="VFV99_33215"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5064298.1"
                     /translation="MGTPKRKRRIQGKPGRSSLADALFTRTQQKVLGLLFGQPERSFG
                     TLELIALASSGRGTVQRELERLVHSGLVRVDRVGPQRRYQANALAPIFHELRGIVEKT
                     SGIPEALRRALEPLASKIALAVLFGSVAKASENALSDIDVLVVSDHLTLEPLYAALEP
                     AERALGRRVSPTLYTTAEYSARRRNKHPFVTKVMEGKHVVLVGTEDGEQEAR"
     gene            15597..16019
                     /locus_tag="VFV99_33220"
     CDS             15597..16019
                     /locus_tag="VFV99_33220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064299.1"
                     /translation="MANKKLDNLVTIGKLKPEPPAQVELDGLIASGTARLADAKNEEL
                     SFESRFDLAYNAAHSLALAALRFHGYRSEARYLVFQTLEHTLELPSALWRILDQAHGA
                     RNRSEYEGYLDKDQQLLAALLRTADEVEKRVRALLGAK"
     gene            16116..16610
                     /locus_tag="VFV99_33225"
     CDS             16116..16610
                     /locus_tag="VFV99_33225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064300.1"
                     /translation="MLLRAVLAAFVLAGCAADARSLDGWRGQYRVDSSSCAFVDGTTP
                     VRLHDPEPGTLAIYAFMPPDADYRLDGVTLEADGNRGLTGSAVTLLCGPPCSSCTREV
                     RYERDDVTTTSSTIPPTTIHLQLLRASEPATEGTCTEDRAWALAASAVPCMVDATYYA
                     ADHI"
     gene            complement(16665..17036)
                     /locus_tag="VFV99_33230"
     CDS             complement(16665..17036)
                     /locus_tag="VFV99_33230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064301.1"
                     /translation="MPWWLPVLTIVGTTCWLTADEIAMAIRERCAARRRARHEPVDAW
                     IIELDGEDIGLLVQPVVTDMFWKRFVVVGEDPRLFDNTLWEQCRFRYRHAWSGRRAEH
                     AFCGGLRPTRDAPHVSMRGLC"
     gene            complement(17144..17422)
                     /locus_tag="VFV99_33235"
     CDS             complement(17144..17422)
                     /locus_tag="VFV99_33235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064302.1"
                     /translation="MVYVLLAAALLAPVAAVAVAQLLAEGRFGNKITAHGWAGLGGCF
                     VAGQLTRQLLGVTIWSGVAGVGAAIAVGLVARAMAKRSKREDETSRPQ"
     gene            complement(17461..17916)
                     /locus_tag="VFV99_33240"
     CDS             complement(17461..17916)
                     /locus_tag="VFV99_33240"
                     /inference="COORDINATES: protein motif:HMM:NF024275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064303.1"
                     /translation="MRTAIALALSLSLFAFAGCKKKEGGDKAAPKAGETAAKPDEAKP
                     AAGPAQMEAPALFDDYNKPGQDGLALLDKWKPGVIVTGTVTNVITEEAGNSHVWVDGG
                     NNHKVTLDFTDQGKAAKDKGVKAGDKVTAQCSIGGSDGNMMMLTDCTLK"
     gene            18061..18711
                     /locus_tag="VFV99_33245"
     CDS             18061..18711
                     /locus_tag="VFV99_33245"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX5064304.1"
                     /translation="MRSPSILLLATLGGCIVAKQSLEAPPNDETTVMLFTGGLGHPMD
                     GIARHPWFAVREKGATEWEIYEVGGGGSQSNPADNHPYVDPILHKVWRGEDGQRAAEC
                     IRREGPNAKSYIESHYIFYPGPNSNTFGDVVLRRCKLSASLPSTSVGKDWRGIIGGGV
                     TSERTGVQVETPVLGVKLGLKEGIEVHVIGLSFGIDLWPPAIILPLGPGRLGFADR"
     gene            18708..>19198
                     /locus_tag="VFV99_33250"
     CDS             18708..>19198
                     /locus_tag="VFV99_33250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064305.1"
                     /translation="MRAPHVIVGLALVIGTHAASAQPDVAGGAQLHYELEGFHDIEQH
                     DAMSVPSVRNIVLASARLHGYIGSKTIGFHMGLDLGAGGTIDGGGFAYDVSLFPVGVA
                     FRFAETSFLTFGAGVGASGATGVMDDAATLPLEARFEIGRGIRVLGRARATFLAAADN
                     RQDG"
BASE COUNT         3335 a         6181 c         6547 g         3135 t
ORIGIN      
        1 gattcgcggc gtggtggccg atgtcgtgcg cgcgcccgag ttgctcgagg tcgcggtcga
       61 agcggcgctc ggcgatcgcc tcggcggcgt gctcgtcagc gagcccgaag tcggtgtcgc
      121 tgcgatcggc ttcttgaagc agggcggcgg tggacgcagc gcgttcgtgc cactcgagac
      181 ccggagcgcc gtccctgtcc tcgtcgggcc gacggcgtcg acgcccgtgc cgttcgaagg
      241 cgagggcggt ggctcgtcgt ggatgtcgcc cgcgtgggca gccgacgacg acagcccggt
      301 gaagcagacg atcggcagcg ggccgacgtg gctgaacccg gactggagcg acggcaatcc
      361 gttcgctcat aacgagtcgg gcaactccgg caagatcgag gtcgaggatc gcagcgagct
      421 ggttgccgct tcgatcgcga tcgcgaagag cccggagctc gcgggtgaag gcgtgctcgg
      481 tcgtatggcc gacctcgtct cgttcgccga tggctacgag gacgtcggca agcggctgct
      541 cgccggtacg gtcgtcgtcg atcagctcga tcgcgcgatc cggctgcacg agcaaggcgt
      601 caaggatcgg ctcgtcacgc tcgacggcga tgtcgtcgac gaggacggcg tggttgccgg
      661 tggctcgcgc gatgcgcagg gcgctggcgt gctcgcgcag aagcgcgaga ttcgcgacct
      721 cgaagagatc gtcggcaagc tcgagcacga tctgtccgaa gcgatggccc gcctggtcac
      781 cgcgaagacc gagctcaagc aggtgatgaa ggcgctcgac ggtctgcgta cgcaggtcca
      841 cgagggcgac gtcgccatca tgggccacga gaaggacgag tcgcgcgtac gtcacgagct
      901 cgagcgccat cgcgatcgac tcggtcagct cggcaacgag cagctcgagc tcgagcatcg
      961 catccgcgcc atcgagatcg atgacgccgc cgctcgcgag cgcaagacgc aagcgatcga
     1021 gcgcatcgcc gagctcgagc gcgcgcagct cgacctcatc tccgaggtct cgtcgcaccg
     1081 cgaccagctc gagcaactct ccgcgtcgct caccgaagcg cgcgtgcgcg ctgctcagct
     1141 cggcgaaaag cgcgccgcgg ccgaggcatc cgcactgcgc ctgcaaacaa ccgacaacga
     1201 gctcgccgct cgcgccgatc gcctcacgca ggaaatcgac gaggcggcgc gccgcgcagt
     1261 cgatctgcgc gctggctgcg atgcgctcgc cctcgagctc gaagaggtcc gcacgcgtcg
     1321 cgaggcgcag gtcaccgagc tcgatcaggg ccgcgtcgcc tacgaccagc gcctgcaagc
     1381 gctgaccgag gtcgagctca ccgcccgcga gctgcgctcg caagccgaca agctcgcgca
     1441 ggagctgcag cagctcgagc tgcgcctcgg ccagatcaag atgaccaagc aggtcgtcca
     1501 tgacggcgtc gacgagcgct accagatcga gatcacggac gtgctcttcg attatcacca
     1561 gcgctcgccg atcaccgagg tcgaagatgc ccggctcgcc gagctcaaag acctcatcga
     1621 tcgcatgggc accgacatca acctgaccgc gatcgaggag ttcagcgaag tttcgacgcg
     1681 gttcgagttc ctgtccgccc aaaagatcga tctcgagcgc gcggtggatc agctgcagcg
     1741 cgcgatcgac aagatcaaca agaccagccg caagctgttc aaggacacgt tcgccgcggt
     1801 taatgcgacg ttcaaggaag tgttcccgcg gctgttccgc ggtggccaag ccgcgctgtc
     1861 gctgtccggt ggcgaaggtg tcgacttgct cgaggccggc gtcgagatca tggcgcagcc
     1921 accaggcaag aaaaactcga cggtcgatca gctctccggt ggcgagaagg cgctcaccgc
     1981 cgtcgcgctc gtgttctcga tcttcctcat caagccgtcg ccgttctgca tcctcgacga
     2041 ggtcgatgcc ccgctcgatg aagcgaacgt cgatcgctac aacgagatcg tgcgcgagat
     2101 gacggaccgc tcgcagttca tcgtgatcac gcacaacaag cgcacgatgg aatcggccga
     2161 caagctgtac ggcgtcacga ggcaggaacc gggcgtgtcg aagctcgtct ccgtgaacct
     2221 ggcgaagctc ggcgcgcaga aggcgaacag cccgcagtcc gtcgcggtct agcggcaggt
     2281 gtcttcgctg tcgtcgccgg cggtgatgcc gcaccggccg gacacgcagt ctgcgtcgaa
     2341 gtagcagcgc gcggcgactg cgcattgttg acagttcgcg ccgcagtcga tgtcgccatg
     2401 tagagcgcgt agcgttgcgt cgtgccggtg aacggcccga cgacgacgtc gcttgtcgag
     2461 cacccgacga gcagcgcagc aagaacaacg gtgcgcatgt cagcccaata ctgcctcggg
     2521 cgtgacggtc cggcgacggg caggaccggc aggttccggc gagatcgagt gcacaactca
     2581 tgtgagatcg agggtcttca tctgcaacca gcacgttccg gcacgttttc ggcgtcgcat
     2641 gcgcgagcgc gacgtaatga gccccaatct tcgatacgcg cgatcgggtt gcggcgacgc
     2701 tgtcgcggtt agcgcctgcg agcggcacgt gtaggtgccg cgcgaaacgc acgttcgcgg
     2761 cgtcatcgtc tgacaaatgg cttttcggga tccgccgcgt cgagatttcc cttacgctga
     2821 cggtggtgag gtttgcgcgg tggcaaatcg cggtggcgtt ggttgcgggg tgctatgcgc
     2881 cgaacgcgaa catcggcgcg ccgtgtccag acggactgtg tccgagcggg cagatgtgcg
     2941 acgtcgatca tgttggcggg ccgacgtgcg tgttgccggg caccggcggc agcggcagcg
     3001 gcagcagtga agtcgacagc ggtggcggcg aaacgttcta cgacgcggca ccggaagaca
     3061 cgatgacgat catgatgccg gacgcgcccg acctgccgcg gccagcgaac gattcgcccg
     3121 cgaatccgat cgatgtgtcg gcgggcggca tgttcatgtg ggatccgacg aacgcgctcg
     3181 acgatgtcgc gtcgccgtgc gcagcgagca ccggccccga cgtgttcttc acgatcacgc
     3241 tcgcggcgcc cgaggtgatc tacttcgaca cgtttggctc gtcggcggac tccgtgatcg
     3301 cgatctatcc cggcgactgc acgccgctcg gcacgataga agcgtgcgtc gacaacagct
     3361 gcggcgggac caagtcgcag ggcgcgtgga atctgaccgc gggcacgcac tgcatcgtcg
     3421 tcgatcaggt gggcagcacc ggcggcacga ccggcaagct caacgtcgtg catggccggc
     3481 gcgcgggcga tccgctggtc ggcgcctccg gcacggtgtc tggcgacacg tgcaacgacg
     3541 acaacagtaa caacgcatcg tgcggctccg agcccagctt ggatcaccac tacttcgtga
     3601 cggtgtgccc gaacagcgcg ccgaacctgc acatcgagac gtgcaaccgg gcgaactggg
     3661 acaccgtgct gcagattcgc tcgaacgcga cgagcggaag cacgctccaa tgcggcgacg
     3721 acagctgtga caatacgcgg acgtcgatcg atgcgacgtt gaccggcccg ggattgttct
     3781 gggcgatcat cgacggctac gatgactgcg gtcagtacac gatggcgttc acgctgcagt
     3841 agcggctccc gatcagcgga cgctgcagta gccgccgccc gatgagccac gtctgcccga
     3901 tgcggcagct gtggcgcgtc tgcccgatga ggcagctgtc ccgcggttgt ccggcgcggg
     3961 tgctatggtc agtcgatggc cgagcccacg cagccggatc gcgaaacgcg cgaacagttc
     4021 ttgggcgtgc tcgctgagct gatcgcgcag ggcggggcag agcgcttcct gctgccgccg
     4081 gtcgcgccgg gtggggatgc gtttcccgag ccgtgggcac cgacaacggc cggcgtgcag
     4141 ctgctgctgc gccggctgct gtggcacgcg ggcatcgagc gcgccgtcga gctcgacgac
     4201 gatcgccttg cgaaggcgcc acccacggag cgcaagccgg caacgcgcgt cgagatcacc
     4261 gagctcgcga agacaacgcc cgcaggatcg aaggcggcgg gctcggcgcg gttcaccgtg
     4321 tacttcatcg gtgaggacga gatcgcgggc acgctcgcgc acgaggtcgg cacgctgtac
     4381 gcagcgctgc accgcgtgga gaaggcagat ccgtatcgca cggccgaacc gccgacgatc
     4441 acgatcgatc cggatcgcga tctcgagcgc ggctcgatcg cgaccgtata tctcggcctc
     4501 ggcgtgctcg cggcaaacgc tgcgttccag cagtacagca gcgcgggtcg cttcaacggc
     4561 ggctacgtgc cgctcgagta cgacgtcttg cgcgccggtg cagtgccgat gtccgcgctc
     4621 gcgtatctgc tcgcggtgca ggcgaccgtg cgtggcgacg acgcgccacc tcgcgggctc
     4681 gcgccgccgc agcgcgatga agttgccgac tggatgaagg cgctcgatgg ccaaggcgcg
     4741 acgctccgcg agcgactcgg catcgcggcg agcactgccg tgcaggcgcg gcccgccgtc
     4801 gagccgctcg atgacgagct accgccgttc gaagagccgg cgccgaagcc gactgcgttt
     4861 cgctggcaga cgcaccgtgg gggcgtaggt ttcgttgccg gcaccgtgat gggagtgggg
     4921 ctcgcgttcg cactcgcgcg cggcatgttg ccgctgacgc tggtcggcgg cggaacgctt
     4981 gggcatctga tcggtcgccg cgtgcgcgcg ccgcggtgct cggcgtgcgc gaccgtgttg
     5041 cccgaggggg cgaccacctg cgcgaaatgt ggcgccgtcc tccgcggaga tattgcgaat
     5101 ctgggcgacc ggctcgaggc tgaagaacgg ctcgaagaag aaaatcggga gcagctgtaa
     5161 acctctccct accgtcgcgc gtccttcaaa cagacgacga cacgttgcag cgcgctggat
     5221 gtacgacagg tacgagcgcg cggcaccttt cacgattcct cgacgagaaa atcgatctcg
     5281 ctcacaggat tttcggacct tttagtcgac tcccccggca gagcttggta tttttattgt
     5341 gggagttcct tcgaccgcag gaggtaacta agcatgggta atctgaacga aatcaaggaa
     5401 cggcatggtc gggaccgtct gaaggacgcg ggcttcattg ccgccgcgct tctgctgacg
     5461 gcactcgcaa ttgggtcggt gacctcgaag gcggctggca agccccacac gcacgagtgg
     5521 aagctctcgg tggtggatcc ggtcagcaac gtcgagattg gtcgctaggc ttctgcgtta
     5581 ggccgtccgg ttcccggaca ttcgaggtca tcaggtcgcc aggcgtcgcg ctctagagca
     5641 gcgacgcggc ggccgaccac caggcagccg agaggcagcg acgggcaatc actgcgctgc
     5701 gaccggctgc gcctcggcag ggcgttgatc aacgcgcaga ccacgctgcg cctcagcgga
     5761 cgttgagcag cgcgcagact acgctgcgac gcggcgggcg gtgagcagcg cgcagactac
     5821 gctgcgacgc ggcggacggt gagcagcgcg cagactacgc tgcgacgcgg cggccgttga
     5881 gcagcgcgca gactacgctg cgacgcggcg gccgttcagc agctgatcga gcgatacgat
     5941 cggcgagcct tcgagcaccg tgatgccgtg cttcacgagc aggcgacggc ctggctcgag
     6001 cgcagcgcgc tggtcgcgga cgatgagctc gcggatgcgg cggccgttcg gcgtcgtggc
     6061 ctgcggcaag aacgcgttcg cggacggcgc gatcgcgatg acgctgccgg ctgacttgcg
     6121 gagccgcgcg agcgcgcgct tcacgcgcac atcgtcctcg gcaaagccgg cgaggtcgga
     6181 gatcaggacc acgacatcgg gccggccgtt cgcgaccgcg cgctcgaccg cggcagcgaa
     6241 gccgtgggcg cggcggccgt gctcccacgt cgtgcgatac ggcagctcga taccgcgcag
     6301 gcggcagaac tggcgcagtg gccgcagctg cacgtcgtcc tccgcgacat gcggcggctt
     6361 catcttcttc tggccctctt tgccgagcat gacgtcgatc aggcgcttgc agaggcggcc
     6421 cgtcgcagcg acgtcgtaga gctgaccgtc ggggccggcc tggatctgca tccagcgctg
     6481 gtcgtcgagc ggcggcgcga ccttgatgcg gacgtcgatc gcttcttgat gcgcgaggta
     6541 gcgcgcaaca agcgcgacga gctcgcccgc ggtgacgtcg gtaagatccg cgtcgaccac
     6601 gctgcggatg tcgagcatgc ggtcgatgac ctgcaggtag tgatggtggc cggtgtcggg
     6661 cgcgagctcg acgagcggcc gggtatcgaa gccgacgagg ccgacgcgat cgctgttgcc
     6721 gatcgctgcc ttcgcgaggc tcgtcgtcgc gtcgcacgcc cagtcgatcg gcgaccgacc
     6781 gagtgggcct tgccgcatgc ccgcgccgac gtcgacgagg atcacgtgcg tcgtgacgat
     6841 ctcgttctcg aggtcacgca ccatcagctg ctggcggcgg gccgtcgcct tccacgcaat
     6901 gaacttgaac ggatcgccgt gcgtgtgctc gcggagctcg cgtagctcgc cggacaggcc
     6961 gcgccggcgc acgtgatgca ggccgacttg ctcgtgcagc gcgccgccga ctgcgcgcgt
     7021 gatctggcct cgcagtgcca tcgtgcgcgg gaacactttc accgcgatcg ggttcgggaa
     7081 gaacgcttcg atgtcgaaca ggccgagcgc atcgccaaac gtgagcgcgg cgccgtgaag
     7141 cacgtgatag ccggcagtca gcggtttcac ggtcgtggtg acctccacct gctgaccgcg
     7201 atggacggtc gcgctcggcc gctcgtcgat gccgagaccg ctcgcgccga gaatattcgt
     7261 cgacagcacg cgcagccgcc ggctgccgtg gttgcggaac gcgagatgga gctggaatgg
     7321 acgatcgacc gccagcgcac cgccgaattg atcgccaggc ggcacccacc acgacagctc
     7381 gatcttcttg cggcgtagca agatcgccgt cggatagaac atcaagtaaa gcgcgagcag
     7441 caccgagagg atcgcgccgg ccatcgcgac gagcggaggg gcgccgtgca gcgcaccgac
     7501 gatcaagaac agcagtgccg tcccgagcac gagcttcccg cgcgtggcga gacgagggat
     7561 cacgctacca ggcctcgggc ctcaggcttt aggcctcggg cagagaacac ggaattcgca
     7621 tgattcgatc gcatcatcgt cattctgtcc tgacgctcac cggtctgcac ctgaggcctg
     7681 aggcctgatc ggcctactgc ctcttcggca tcttgtagcc gaccttgtcg agcgactcgg
     7741 cgacgaccgc ggtgccggcc gcaccttcga gctcggcttc gggcgtcatc aagatgcggt
     7801 ggccgagcac gagcggcgcg aggtgacgga cgtcgtcggg cagcacgtag tcacggccgc
     7861 ggagcagcgc gaggcccttc gaagcatgga cgagcgcgag gcccgcgcgc ggcgacgcgc
     7921 cgaggtagac ccgcgcgtga ttgcgcgtga acgcgacgag gccgagcacg taatcgacga
     7981 gctcttcggc aacgaacacg ttttgggcga gcgcctgcat ctcgagcacg tcgttgggcc
     8041 cgacgaccgc gcgcaccggc ggcggcatcc ggtcgtacgc gtgcagcatg cgcttttcgt
     8101 cggcctgcac cgggtagccc atcttgagct tgatcaggaa gcgatcgacc tgtgcttcag
     8161 gaagtggata tgtgccttct tgttcgatcg ggttctgcgt cgcgagcacg atgaacggct
     8221 ccgacagcga ccgcgtctcg ccttcgatcg ttacctgctg ctcctgcatc gcctcgagca
     8281 gcgcggactg cgtcttggct ggcgcgcggt tgatctcgtc gccgagcagc acgttcgtga
     8341 acaccgggcc ctcgcgcagc acgaactggt tcgtacgcat gtcgaggatg tacgtgccgg
     8401 tgatgtcgct cggcaggagg tcgggcgtga actggatgcg ccggaagccg cagccgagcg
     8461 tcgccgagaa tgctttgacg agcgtcgtct tcgcgacgcc gggatagccc tcgatcaaga
     8521 cgtggccgcg agcgagcagc gccgtgagca acgcctcggt gatcgtcggc gatccgatga
     8581 acgcacggcc cacctcttcg gtgatcgcgc gcgcgagctg gttgacgcgg ctgagcttgt
     8641 cgtgggtaag ggcggcttgc attaggcggc ttcgggggcg gcttcagtca ggggagcacc
     8701 aagagtacga cacagctcgg cgacatctcg atatagcgca tcgaaatcgc ggcgggcgag
     8761 gtggcccgag ctccaggggg ccgcggcctg accacggctg ggcagtgcgc gcagccgctt
     8821 gtacacgcgc gcgagcgcat ctccggctcc tcggcccctg gcatcggaga cctgcttcac
     8881 gagctgcgcc tccgacagcg tgtacagcgg ctctggcttc ccgagcgcat cggcgagcag
     8941 catctggacc tgatcgcgca ggatgcaacc gagcacgagt gccgagcctg cgttgcgttc
     9001 gctcgccgcg acgagcgcga acggttcgtc gcggcgcgcg gggcggccga agcgaagcca
     9061 tgcaccgtcg atacgcggac cacgccgcac gggaagtgcg agcacggcga gcagcagcag
     9121 cagcgcagcg agcgtggcgg cgagcgcctt catcgcagtg ggcgtgagca accactcgcg
     9181 gcgttgtgac agccaggtgt tgagatcggc gatcgagcgg ccgagcttgc cgccgcgcgg
     9241 atcgttgatg aacggccgcg gctcgccgta catcggcacc ttgccgcgca ggagcacgac
     9301 gcggcgcgcg cgattgccgg tctcgtggcg atcgagccag ctcagcatgt tcgccgccag
     9361 cgtgacgttg ccggggtact gcaacatgcg gttgatgaag atgctcggat cgctgaccgc
     9421 gacgaacctg cccgtgccgc gctccccggc gacgaccacc gcgccatctt cgaagccgac
     9481 gagcgtcgtg gcgccttcca cttcccgaag cgcagccggg tgattcgtga cgacctcgtc
     9541 gacaccacgc gtgatcggat ggtcggcctg gacgctcgcg atcggcgccc acacgcggcc
     9601 gtcctggtac ttgctcgcct tcggcgtcgt gacctcggcg cgcaagaggc cgaggcgcga
     9661 catcgcttct ttgccttcgc cgaagtcgtc ggcgatcacg acattgccgc ccgcttgtac
     9721 gaacgcgtcg acgcggctcg gctcgacgtg ctcgagcgga tagaccatga acaagatgtc
     9781 gttcgcgctg agctcgcccc actcgagcgt gctgactgcc gtcacctcgt agcccatgcc
     9841 ctcggcgagg ccgacgaaca ccgccatgcc gttccacgcc gggctgcgcg gatcgtagtc
     9901 ggggatgtcg tcgacctgcg cgcgggcagt gcctgccagc gtggcaacga gcgcgagcgc
     9961 gaccgcgacc agcgatctca tagccccatt tccctcctca gctcgacaga cgccgtgcgt
    10021 agctgttgca gcgcgtagcc gagattcgcg ggctcggtca gcgcgagcag cgcgtagtac
    10081 cccgcgtgca cggcgacaac cacgatctcg agctcgcgat gctgtgcgat gaagtactcg
    10141 gggccgccga agtgccaggt gccgaatgcg gcgttgaggt gcgccatcac gacgccgtag
    10201 tgcgcggtga gcactgccca ctcgtgcgga tcgccgatgc agtagctgtc gaccatctcg
    10261 ccgtcggcgt cggcaaatgc gccgccgatc gcattcggcg tcgcttgcac cgcgcgctgg
    10321 aggatctgcg cgaacggcgt cacagcttct ccaggactgg gaggttcttg tcgatgatct
    10381 ctgcgtgcag cccgaggccc gacgcgatct tgtcgaccca gtgcgtgaag tagctgcgct
    10441 tgacgtcggg gagcgcttcg gtgacgagct gttcgccgct gagatgcacg gcggggacga
    10501 tcgagtcgag caggatgacg tcccagcccc acggcgtgcg caccggcggc gcgacgcggc
    10561 cgatctccgg aatcgcgaac agcgcgccgg tatacgtctc gtcgagctgc ccgtgttgca
    10621 ggcttgcctg gacggtcttg atatcgagcg gtgcacgggt gccgatcacg cgcgtcgcga
    10681 tctcttgcac gtgtggcgcc atcaagccgc gctcgtcctt cagcgctgca tagacatcgt
    10741 cggcgatctg cttcgccgcg gcatcctgct cgggggttgc ctgcttcggc acgtcgatgc
    10801 gcacgtagac cgagccgcgc gcttcggggt gatcgaagcg gccgcgattc ttgtcgacgc
    10861 tgcgcgacca gtacgtaccg aactcttccg gccgattgta cttgtcttcg tactcacgtg
    10921 cgacgaggtc gctgacgagc gcagtccgcg tcgcgagcac gacgtcgcga tcggtcgtga
    10981 gccctcgctt ctcggcctcg attgccatca gctcgaagtc gatgcactgc ttcaatgctt
    11041 cgtcgcgtgt cgcaccgcgc gctgcttgcg cagtcacgca cgagccccag acgggacggc
    11101 cctcgaccgt cgcgacctga acgtcgtcgt gcgtggtcgc gatcatcggc gcaggtggcc
    11161 ccgagcgcgt cggtggcggt gacgtgtgcg agccgctgca cgctgcgagg accatcggaa
    11221 tcgcgacgag ccggcgcatg aatcagtcgc gttgcaccag cgcgaggcgc gccatttctt
    11281 tgccgacgga gatcacggcg acgccgatca ccatgacgag gagcgcggcg acgtagtcgc
    11341 gtctcgcgag gtaatcgagc gtctccttga agaagaacag actgccggcg atcagtgcga
    11401 tggcgaggat ttcgaagaag tagcgacgca tgatcacctc acagatgacg gagcaggatg
    11461 agcagcaaca tcgcgccgag cgcgagtgcc accgacagat agaaacggcc gcgcggcttg
    11521 ccggccttgg catcggcgcg cgcctgggcg agcgcaccga ccagatcgcg atcgccggtg
    11581 cgatgcggac gcagcgcgcc gaggaacgcg tagtgataga tgccgagcca cagcacgatc
    11641 gccccgacgg tgagcgcggt cgtcatcagc gcgacgcggc cgggatacat ctcgccgagg
    11701 aagtcctgga tcgattgatc gaagttgacc cacgcgatca gcactgtcgg gatgagcagc
    11761 gacatcacgg tcagcatcca gtacacgcgc tcgcgccgct gccagcgcgg ggcagggccg
    11821 accgccgccg acgatagcgg ccgtatcgac ttcggcgcgg gcggctcgtg gaacgtggtg
    11881 aagaacgaga cgccgaacag gtaggcgatc acgccgagca cgacgagcac gaagcggatc
    11941 ggcccgtaga cgtgcgacga cgcgagcgcc gggttcaagc tgatcggcac gagcagcgcc
    12001 gacggaaata gcccgagcag gatcagctcg ctgcggcgcg cgaggccgag catcagcatg
    12061 ccgaacggca gcatcgcggc gaggatcatc ttgccgcgca gcgcggcgac gaacactgcg
    12121 ggcaggatca cgatcgtgat ccacacgttc acgccgagcg tgaacgcgac gacgtccgcg
    12181 aacgtccgcc acttgcgttc ggtgacctgc tccgtcacag gcctcgaatg taccgcgcgg
    12241 gactccatga tgccgccgac ttggacagcg tcgtgggacc cctgggtccc gcgaaagttg
    12301 aggtatatac ggcgaccatg cgccgcatcg tcgtcacgat cgatgggccc gccggagcgg
    12361 gcaagtccac cgtcgccaag cagctcgcgc ggcggctcgg ctatcggttg ctcgacaccg
    12421 gcgcgatcta ccgcgcggta gcgctggtcg cgaagcgcag cggtgtgccg tggaccgacg
    12481 ccgcggcgtg tgcggcggtc gcgcgcgacc tcgacatctc gtatcagttc gtcggcgata
    12541 agaaccacgt gttcgtctcc ggcgaggacg tctcgacggc gatccgcacg cccgacatca
    12601 gtcagggtgc ttcgcaggta tcggcgcatc caccggtgcg cgccgcgctc atggatatcc
    12661 agcgccgggt cgccgaaggc ggcggcttgg tggtcgaagg tcgcgacacc ggcaccgtcg
    12721 tgttcccgaa ggccgaggcg aagttctttc tgaccgccac ggtcgaggag cgcgcccgcc
    12781 gtcggatcgc cgagctcgag gcgacgggag cgaccgtcga ccacgagcag acactgcgag
    12841 aaatccgcga gcgcgatgaa cgcgatgcga gccgcgacgt agcgccgatg cgcccggccg
    12901 acgacgctgt gctggtcgac agctcgacgc aaaccatcga acaggttgtg gaaaatttag
    12961 cccgcgtagt cgaggcgcgc gccgacgctc gttagcgagc cgggcaatcg agcggatctc
    13021 atcgttccgg ttgacatttc agggcgcgaa cggtaccaag tgcgccctgc cttgggactg
    13081 cttaggcggt caaatctaac caaggcagct gtaggagatc tgcttgtata tggtgagcca
    13141 agaaggccaa ggcgatgaag actttgccgc gctgctagcg gaaagcttcg gccaagagaa
    13201 cgtcaaggag ggtgaaatcc tccgcggcac cgttatcgcc gttggtaaag atttcgcgat
    13261 cgtcgatgtc ggttacaaga gcgaaggcca agtcccgctc gaagagttcc gcatggcgga
    13321 cggctcgatc ggcgtcaagc cgggggacgt cgtcgacgtc ctgctcgagt cgcgtgagaa
    13381 cgacatggga atggtggtcc tctcgaagga gaaggccgac cggttcaagg tgtgggacga
    13441 gatcagcgcc gcgtgcgaac gcgacgagct gatcgagggc atcatcacgg cccgcgtgaa
    13501 gggtggcctg tcggtcacga ttcgcggtgg cgtgaaggcc ttcttgccgg gttcgcaagt
    13561 cgacctgcgc ccggtgcgca acctcgacgc gttcctcggc cagaacttca agttcaaggt
    13621 catcaagttc aacaagaagc gcggcaacat cgtgctgtcg cgccgcgtcc tcctcgagaa
    13681 ggagcgcgct gcactgaagg agtcgacgct cgagcgcctc aaggaaggcc agctcgtcga
    13741 aggtatcgtc aagaacctta ccgagtacgg tgcgttcatc gacctcggcg gcatcgacgg
    13801 tctgctccac atcaccgaca tgagctgggg ccgggtcaac cacccgtccg agctgttcca
    13861 ggtcggtgat cacgttcgcg ttcgggtcct caagttcaac gcggacaccg agcgcgtctc
    13921 gctcggcctc aagcagatca gcgaagatcc gtggagccgc gctgctgaga agtacgtgcc
    13981 tggcacggtc gttcgcggca aggtcgtctc gctcaaggac tacggcgctt tcatcgagct
    14041 cgaagaaggc atcgagggtc tcgtccacat ctccgagatg tcgtggacgc gccgcgtgaa
    14101 gcacccgtcg aagatggtcg ccgtgggcga catggtcgag gcggtcgtgc tcgacgtcga
    14161 cgtcaagcag aaccgcatct cgctcggcat gaagcagctc gagccgaacc cgtacgagca
    14221 gctcaccgag aagtacccgc cgggtaccgt cgtgaagggc cacgttcgca acatcgccga
    14281 cttcggtatc ttcgtggaga tcgaggaagg catcgacggc ctcgttcaca tcagcgacat
    14341 gagctggacc cagcgcgtga agcacccgag cgagctgttc cagaagggtg acgaagtcga
    14401 ggcagtgctc ctcaacatcg acaccggtga cggcgacaag ccgaagatca gcctcggcat
    14461 caagcagctg gttcaggatc cgtgggatcg catcccgcag gagtacccgg ccggcaaggt
    14521 cgtcgacggc aagatcctca aggttctcga cttcggcgca ttcgtcgagc tcgagaaggg
    14581 catcgagggt ctcgttcacg tgagcgagat cagcgaagag cacgtcgagg atccgcgcac
    14641 ggtgctgcag ccgggtcagg cagtgaaggt ccaggtgctc cacgcggaca gcgcggagcg
    14701 caagatcggc ctgtcgatca agggcgcgaa ccgcgcgaag gagctcgcgg atgcgcaggg
    14761 ctacgcgtcg ccgaacgccg gcggtgcaac gctcggcgac aagttccgcg gcaagctcga
    14821 caagctgaag agcggcgcga aggacgcgca gtaatcagca gcgcgaggca ctagcctcga
    14881 gcaaacaacg gctcgcctcg cggtgggccg ttttcgtttt cggcagcgcc aaccaacaag
    14941 gacttgcctg gtacccaaag tgggtactaa gatacccata atgggtactc caaagcggaa
    15001 gcgtcgcatt cagggcaagc caggccgtag cagcctcgca gatgctctgt ttacgcgtac
    15061 ccagcaaaag gttctcgggc tcctcttcgg tcaaccagag cgaagctttg gcaccctcga
    15121 gctcatcgcg ctggcaagtt caggacgcgg aaccgtgcaa cgcgagttgg aacggctggt
    15181 acacagcggg ctcgtacgtg ttgaccgcgt tggtccgcaa cgacgctatc aggcaaatgc
    15241 gctcgcgccg atctttcacg agctgcgtgg catcgtcgag aagacaagtg gcattcccga
    15301 ggcgcttcgg cgtgccctcg agccactcgc atccaagatc gcgctggcgg tgctgtttgg
    15361 atcagtcgcc aaagcaagcg agaacgctct cagcgacatc gacgttctgg tcgtctccga
    15421 tcatctgact ctcgagccgt tgtatgccgc gctcgagcca gcagaacgtg cgcttggtcg
    15481 gcgtgtcagc cccacgctgt acaccacggc ggaatactcg gcgagacgac ggaacaagca
    15541 cccgttcgtc acgaaggtaa tggagggcaa gcacgtcgtg cttgtcggta cggaggatgg
    15601 cgaacaagaa gctcgataac ctcgtcacga ttggcaagct caagcccgag ccgccagcgc
    15661 aggtcgagct ggatggactg attgcatctg gcactgctcg actcgccgac gcgaagaacg
    15721 aggaattgag cttcgagagt cggttcgacc tggcgtacaa cgcagcccat tcgcttgcgc
    15781 tcgctgcgtt gcgtttccac ggctaccgtt ccgaggcccg gtatctcgtg ttccaaaccc
    15841 tcgagcacac gctagagctg ccgtctgcgc tgtggcgaat cctcgaccag gcccacggcg
    15901 cccgcaatcg ctccgagtac gagggctatc tggacaagga ccagcaactg ctcgctgcgt
    15961 tgcttcgtac ggcggacgag gtcgagaaac gcgtacgcgc gttgctcggc gcgaagtaga
    16021 gtcggagaca ccgtgcggtt agctcgcctc gtgcgggccg gtttcgcttt cgggcactgc
    16081 tcggcacgtt ccaggcgtat cctcgatggg gcgtcatgct cctgcgtgca gtactcgcag
    16141 cgttcgtgtt ggcaggctgt gcggcagacg cgcggtcact cgatgggtgg cgcggtcagt
    16201 accgcgtgga ttcgtcgagc tgcgcgtttg tcgatggaac gacgccggtg cggctgcacg
    16261 accccgagcc cggcacgctg gcgatctacg cgttcatgcc gccagacgct gactatcggc
    16321 tcgacggggt cacgctcgag gcggacggta accgtggatt gactggcagc gcagtgacac
    16381 tgctgtgcgg cccgccgtgt agctcgtgca cgcgcgaggt tcgctacgag cgtgacgacg
    16441 tgacgacgac gtcgtcgacg ataccgccaa cgacgatcca ccttcagctc ctacgcgcga
    16501 gcgagcccgc gaccgaaggg acgtgcaccg aagatcgcgc gtgggctctc gcggcgtcgg
    16561 cggttccgtg catggtcgac gcgacgtact acgcggccga ccacatctga cccgcgccgt
    16621 cactactacg cggtcgacca catctgatcc tctgatccgc accgtcagca caagccgcgc
    16681 atcgatacgt gcggtgcgtc gcgcgtcggg cggaggccac cgcagaacgc gtgctcggcg
    16741 cggcggccgc tccacgcgtg gcgatagcga aaccggcact gctcccagag cgtgttgtcg
    16801 aacagccgcg ggtcctcgcc gacgacgacg aaccgcttcc agaacatgtc ggtgacgacc
    16861 ggctgcacga gcagtccgat atcttcgccg tcgagctcga tgatccaggc gtcgacgggc
    16921 tcgtggcgcg cgcgccgtcg cgctgcgcac cgctcgcgga tcgccatcgc gatctcatcc
    16981 gcggtgagcc agcacgtcgt ccccacgatg gtcagcacgg gcagccacca cggcacgcct
    17041 ctccggtgcg cactacacaa ccgacgagca acctgcctgt tcgggctact tcgaaatggg
    17101 gtctgaccca cttctgcgct gagctagacg tcggatcgga ccatcattgg gggcggctcg
    17161 tttcgtcctc acgcttcgac cgcttcgcca ttgcgcgcgc gaccagaccg acggcaatcg
    17221 cggcgccgac gcccgcgacg cccgaccaga tggtcacgcc gagcagttgc ctcgtgagtt
    17281 gaccggccac aaagcacccg ccgagccccg cccaaccatg cgcggtgatc ttgttgccga
    17341 agcgtccctc ggcaagcagc tgcgcgacgg cgaccgcagc gacaggcgcg agcagggctg
    17401 cagcgagtaa gacgtagacc atgctgaagc gagtgtgcct cgccgccgca tttggttgcg
    17461 ctacttcagc gtgcagtcgg tcagcatcat catgttgccg tcgctgccac cgatcgagca
    17521 ctgcgcggtg accttgtctc cggccttcac gcccttgtcc ttggccgcct tgccttgatc
    17581 ggtgaagtcg agcgtgacct tgtggttgtt gccgccgtcg acccacacgt gcgagttacc
    17641 ggcttcctcg gtgatcacgt tcgtgacggt gccagtgacg atgacgccgg gcttccactt
    17701 gtcgaggagc gcgaggccgt cctggccagg tttgttgtag tcgtcgaaca gcgcgggcgc
    17761 ctccatctgc gccgggccgg cggcgggctt cgcttcgtcg ggcttggccg cggtctcacc
    17821 tgccttgggg gcggccttgt cgccgccttc cttcttcttg cagcctgcga acgcgaacag
    17881 tgacagcgag agagcgagag caattgcagt acgcatggag tgtcctttcg tgcgtgggga
    17941 caatatcaag gcgcgtgccg gcgcaacctc gcgatggctg gtgaccgcgc gggtctggcc
    18001 ggacccagac ggcggaaata ttgtgcccgg cctgggcgtc tcgtgcgttc aacgggcgtg
    18061 atgaggtcgc cgtccatcct tctgctcgca acgcttggcg gttgcatcgt tgcgaagcag
    18121 agcctcgaag caccgccgaa tgacgagacc accgtgatgc tgttcaccgg ggggctcggc
    18181 catccgatgg acggcatcgc gcgccatccc tggttcgcgg tgcgcgagaa gggcgcgacc
    18241 gagtgggaga tctacgaagt gggcggcggc ggatcgcaga gcaatccggc ggacaaccat
    18301 ccgtacgtcg atccgatcct gcacaaggta tggcgcggcg aagacggcca gcgcgcggcc
    18361 gagtgcattc gtcgcgaggg accgaacgcg aagagctaca tcgagagcca ctacattttc
    18421 tatccgggtc cgaacagcaa cacgttcggc gacgtcgtgt tgcgccgctg caagctcagc
    18481 gcgagcttgc cgtcgacgtc ggtcggtaag gactggcgcg gcatcatcgg cggtggcgtg
    18541 acgagcgagc gcaccggcgt gcaagtcgag acgccggtcc tcggtgtgaa gctcggactc
    18601 aaggaaggca tcgaggtcca tgtgatcggg ctgtcgttcg gcatcgatct gtggccgccc
    18661 gcgatcatct tgccgctcgg tcccggccgg ctggggttcg cagaccgatg agggcaccgc
    18721 atgtcatcgt cggtctcgcg ctcgtgatcg gcacgcacgc ggcgtcggca caaccggatg
    18781 tcgcgggcgg tgcgcagctt cactacgagc tcgaaggctt ccacgacatc gagcagcacg
    18841 atgcgatgtc tgtaccgagc gtgcgcaaca tcgtgctcgc gagcgcgcgc ctgcatggct
    18901 acatcggcag caagacgatc ggcttccaca tgggcctcga ccttggcgcc ggcggcacga
    18961 tcgacggcgg cggtttcgcc tacgacgtct cgctgtttcc ggttggcgtc gcgtttcggt
    19021 tcgccgagac gtcgtttctc acgttcggcg ctggcgtcgg tgcgagcggt gcgaccggcg
    19081 tgatggacga cgccgcgacg ctgccgctcg aggcgcgctt cgagatcggc cggggtattc
    19141 gcgtgctcgg ccgcgcgcgg gcgacgtttc tcgccgccgc cgacaatcgg caggacgg
//
LOCUS       DASUFX010000824         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_552707,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000824 DASUFX010000000
VERSION     DASUFX010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552707"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFV99_33255"
     CDS             <1..704
                     /locus_tag="VFV99_33255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064306.1"
                     /translation="SGAAAGSAAPAAPAPGGAAPAGAPAPAPDLAAVKPEEMPTAVRM
                     RRLEQKTQALKEKAWQLKARVQMLKEQTLGGGVGAQASIDHHDQMGASYRLTKLIYSL
                     DGAQVFARSDEAAESMYKTRNFDIFAGPISPGNHTLQVIATYRGHGYGVFEYLTKFTF
                     TARGSASFTTGEGKISKVECKGYEKGGAATPMEKRPAIECKVSEAAPEKPAETKPTTP
                     AGSTPLPPPASAPGK"
     gene            708..3149
                     /locus_tag="VFV99_33260"
     CDS             708..3149
                     /locus_tag="VFV99_33260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064307.1"
                     /translation="MKLTVFRSLWAAALALAFASSTAAAAPDVKGLLQSYEAEAKALA
                     TNIPQPNQMSTETGQRRLVDAQVAFSIGDYDGASLVLFDLVGKTQGQDKEIATFYLAE
                     ALYHKGDRGAARQYYTEVAGAVGGKYYVPSLMRLVEISMVDHDTQTAEDAIGKLATAS
                     QTPAVPYVRGKWAFSEGKYDEAIGFFNAVPKGSEYDAQATYYLGTTFIAQKDLARATE
                     IFTELTQRKPKSNTDRRVIELAQLALGRVYYEREQASKSIDSYLLVDRGSDLFPTALY
                     EVSWVYVKSKQYDKALVALELLNRLDPQSTTSPTVRILEGNLRIRKAQLLRHAEITGT
                     LSAEEKSNPAGEYDKAEKIFTETHDRYYPSFVALDRMVQGTLDPASFIDQISGHNTRV
                     FATSAPIPEAAAQWLREEPEVQRVVNVESDLADIQRSINEAEATIVRLEGVIATGDRL
                     TLYPALSSRRMRIAAIQHSLIGIRSQLHDQAGSSDGDRKALVAQYQALGDPEQAHGTR
                     TAEAQQGFDKIEDSAQEVEGAVMSMQAMAVALRTYAPQLPDADRTKLQGEIDDVTKEA
                     RAIEDELADIRAEVVLGKDLAPVGDEDLMRARELRKQVKAALDAEYRNLGGRAGALAE
                     QAARISDQLEAADQNIDAMVGQGLQDVKRMIDGERANIAEYRKLLAEYEQEVRTVGSE
                     VLGFSFKTVREKFDDVITRSDVGNVDVSWSQREDNDDDLKRLNLARARDLKQLRDEFK
                     FILDETATSPAQPAKPKEALPAPGPEGASPDKGGATDQRVKPAGDTKKDATQPTVKPD
                     ASKPGAKPAAKGASK"
     gene            3146..>5584
                     /locus_tag="VFV99_33265"
     CDS             3146..>5584
                     /locus_tag="VFV99_33265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064308.1"
                     /translation="MKKAPLIALLVAATTGLGGIAAGQPGNQTPAPAPAPAPATPANE
                     PPATGPGLGQPTPPPVSGSLQLTPQELEELKGVEADYEQFLEAAKAHDARLRTIARHE
                     FNSRTAELEKRYAERIANAENSKAAKNQVTKERLEKFLADHPKHETFTPDAMFRLADL
                     YLDEADEDVDRRLAEADKNPPDPNKPDAAAIVADYSKSLQLWEDILKQFPNYRQTPST
                     IYLLAYYGKTKDERRSLQLFLALACANRYKWYDAPPPAPDKKEAIKRVESKTLREVYG
                     DCTPYPNADVELVRHAWVRGIADYHFAVPGEIDEAIAAYQKVTNGGQDSKLYAEALYK
                     LAWSYYKRDFLEQSIDNFDKSVVLYDKTVAEGNTPPLELREESIQYIAVAFTDPWLGE
                     TDTDPSKAFERAKKKYAGRENEPHVRDVWVAMGQAFEDLQAWDQAVDSYRIAIGPPWE
                     LNPKNPIVHQKIVNAFESKGDKFAADKAAAELATRYAPGTAWYQANEKDREAMDNQRR
                     IAERALYAAARNTHSAATTLRKDYDAAGAKDPKQKEEYLAMYGQAAELYRQFINTYPE
                     SDYIYEFNFLLGETLFYSEKYREAIVQYKWVRDHRDLGQTYYLDAARSVLASYEAAKD
                     KEVAEGKLQPLKVPTVAELKAIPQPWQPQPIPQIYLELQAEYDNYQNIVQDPAAAPGQ
                     GINAALISLAYFHVDDAIARFTKVMDKFCAAAPMKKGELAPAARAKDGILAIYEAQAN
                     FDAIEATNKRFISAKCGDANAIQLAITQNRSLNFSRAADLYKKQEYIPAAEAFYRFYK
                     TAPQDDPDLPVALYN"
BASE COUNT         1147 a         1833 c         1843 g          761 t
ORIGIN      
        1 gatcgggcgc tgcggctggt tcagctgcgc cggccgcacc cgcgccagga ggagctgcgc
       61 ccgctggtgc acccgcgccc gcgcctgatc tcgctgcggt gaagcccgag gaaatgccga
      121 cggcggtgcg catgcgccgg ctcgagcaga aaacgcaggc gctcaaggaa aaggcgtggc
      181 agctcaaagc gcgcgtgcag atgctcaaag agcaaaccct cggtggtggc gtcggtgccc
      241 aagcgagcat cgatcaccac gaccagatgg gcgcgtcgta tcgcctgacg aagctgatct
      301 actcgctcga cggcgcgcag gtgttcgcgc gctccgacga ggcggcggag tcgatgtaca
      361 agacccgcaa cttcgacatc ttcgccggac cgatctcgcc gggtaaccac acgctgcagg
      421 tcatcgcgac gtatcgcggc cacggctacg gcgtgttcga gtacctgacg aagttcacgt
      481 tcaccgcgcg tggcagcgcg tcgttcacga ccggcgaggg caagatctcg aaggtcgagt
      541 gcaagggcta cgagaagggc ggcgccgcga ctccgatgga aaagcgcccc gcgatcgagt
      601 gcaaggtttc cgaggctgcg cccgaaaagc ccgcggagac gaagccgacc accccggcgg
      661 gctcgacgcc gctgccgcct ccggcaagcg caccgggaaa gtaacccatg aagctaacgg
      721 tctttcggag cctctgggcg gctgcgctgg cgctggcctt cgcgagtagc acggccgctg
      781 ccgcgcccga cgtcaaaggg ctgctccagt cgtacgaggc cgaagcaaag gcgctcgcga
      841 cgaacattcc gcagcccaac cagatgagca ccgagaccgg gcagcgccgg ctcgtcgatg
      901 cgcaggtcgc gttctcgatc ggtgactacg acggtgcgtc gctcgtgctg ttcgacctcg
      961 tcggcaagac gcaaggccag gacaaagaga tcgcgacgtt ctacctggcc gaggcgcttt
     1021 atcacaaagg cgatcgcggc gcggcacgcc agtactacac cgaggtcgcg ggcgccgtcg
     1081 gcggcaagta ttacgtgccg tcgttgatgc ggctcgtcga gatctcgatg gtcgatcacg
     1141 acacgcaaac agcggaggat gcgatcggca agctcgcgac tgcgtcgcag acgccggccg
     1201 tgccgtacgt gcgtggcaag tgggccttct cggaaggcaa gtacgacgaa gcgatcggct
     1261 ttttcaacgc ggtgccgaag ggctccgagt acgacgccca ggcgacgtac tacctcggca
     1321 cgacgttcat cgcgcagaag gatctcgcga gggcgaccga gatcttcacc gagctcacgc
     1381 agcgcaagcc gaagtcgaac actgatcgcc gcgtgatcga gctcgcgcag ctggcgctcg
     1441 gccgcgtgta ctacgagcgc gagcaggcgt cgaagtcgat cgatagctac ctgctcgtcg
     1501 atcgcggcag cgatctgttc ccgacggcgc tctacgaagt gtcgtgggtc tacgtgaaga
     1561 gcaagcagta cgacaaggcg ctcgtcgcgc tcgagctgct caaccggctc gacccgcagt
     1621 cgacgacgtc gccgacggtg cgcatcctcg agggcaacct gcgcattcgc aaggcgcagc
     1681 tactccgcca cgcggagatc accggcacgc tgtcggccga ggagaagtcg aacccggctg
     1741 gcgagtacga caaggccgag aagatcttca ccgagacgca cgatcggtac tacccgtcgt
     1801 tcgtcgcgct cgatcgcatg gtgcaaggca cgctcgatcc ggcgtcgttc atcgaccaga
     1861 tctccggtca caacacgcgc gtgttcgcga cgtcggcacc gattcccgag gcagccgcac
     1921 agtggctgcg cgaggagccc gaggtccagc gcgtcgtcaa cgtcgagtcg gatctcgccg
     1981 acatccagcg cagcatcaac gaagccgaag caacgatcgt gcgcctcgaa ggcgtgatcg
     2041 cgaccggcga tcgactgacg ctgtatccgg cgttgtcgtc gcggcgcatg cgcatcgcgg
     2101 cgatccagca ctcgctgatc ggcatccgct cgcagctcca cgaccaagcg ggctcgagcg
     2161 atggcgatcg caaggcgctc gtcgcgcagt accaggcgct cggcgatccc gagcaggcgc
     2221 acggtacgcg tacagccgag gcgcagcagg gcttcgacaa gatcgaagac tccgcgcagg
     2281 aagtcgaagg cgcggtgatg tcgatgcagg cgatggcggt tgcgctgcgc acctatgcgc
     2341 cgcagctgcc cgacgccgat cgcacgaagc tgcaaggcga gatcgacgac gtcacgaaag
     2401 aggcgcgcgc gatcgaagac gagctcgcgg acatccgcgc ggaagtcgtg ctcggcaagg
     2461 atctcgcgcc ggtcggcgat gaagatctca tgcgggcgcg cgagctgcgc aagcaggtca
     2521 aggcggcgct cgacgccgag tatcgcaacc tcggtggccg cgcaggcgcg ctcgccgagc
     2581 aggcggcgcg gatctcggat cagctcgagg ccgctgacca gaacatcgac gcgatggtcg
     2641 gccagggcct ccaagacgtg aagcgaatga tcgacgggga acgtgcaaac atcgccgagt
     2701 accgcaagct actcgccgag tacgagcagg aagtccgcac cgtcggcagc gaagtgctcg
     2761 gcttcagctt caagaccgtg cgcgagaagt tcgacgacgt gatcacgcgc agtgacgtcg
     2821 gcaacgtcga cgtctcgtgg tcgcagcgcg aggacaacga cgacgatctc aagcggctca
     2881 acctggcgcg tgcacgcgac ctcaagcagc tgcgcgacga gttcaagttc atcctcgacg
     2941 agaccgcgac ctcgcctgcg cagccggcca agcccaagga ggcactaccg gcgccgggac
     3001 ccgaaggcgc gagcccggac aagggcggcg cgaccgacca gcgcgtgaag ccggccggcg
     3061 acaccaagaa ggacgcgacc caaccgaccg tcaagcctga cgcgtcgaag ccgggtgcga
     3121 agccggccgc gaagggagcc tcgaaatgaa gaaggcacct ctcatcgcgt tgctcgtcgc
     3181 tgcgacgaca ggtttgggcg gcattgccgc gggccagccg ggcaatcaga cgcctgcgcc
     3241 tgcaccggca cccgcacccg cgacgccggc gaatgaaccg cccgccaccg gcccgggcct
     3301 cggccaaccg acgccgccgc cggtcagcgg tagcttgcaa ctcacgccgc aagagctcga
     3361 ggagctcaag ggcgtcgaag cggactacga gcaatttctc gaggccgcga aggcgcacga
     3421 cgcacggctg cgcacgatcg cgcgtcacga gttcaactcg cggacggccg agctcgagaa
     3481 gcgctacgcc gagcgaatcg cgaacgccga gaacagcaag gcggcgaaga accaggtcac
     3541 gaaggaacgg ctcgagaagt tcctcgccga ccatcccaag cacgagacgt tcacgccgga
     3601 cgcgatgttc cggctcgccg atctctacct cgacgaggcc gacgaggacg tcgatcgccg
     3661 cctcgccgaa gcggacaaga acccgcccga tccgaacaag ccggacgcgg cggcgatcgt
     3721 cgccgactac tcgaagtcgc tccagctgtg ggaagacatc ctcaagcagt tcccgaacta
     3781 ccggcagacg ccgtcgacga tctacttgct cgcgtactac ggcaagacga aggacgagcg
     3841 gcgctcgctg cagctgttcc tcgcgctcgc gtgcgcgaat cgctacaagt ggtacgacgc
     3901 gccgccgccg gcgcccgaca agaaggaagc aatcaagcgc gtcgagagca agacgctgcg
     3961 cgaggtctac ggcgattgca cgccgtatcc gaacgccgat gtcgagctcg tgcgtcacgc
     4021 gtgggttcgc ggcatcgccg actaccactt cgcagtgccc ggcgagatcg acgaagcgat
     4081 cgccgcgtac cagaaggtca cgaacggcgg ccaggacagc aagctgtacg ccgaggcgct
     4141 ctacaagctc gcgtggagct actacaagcg cgacttcctc gagcagtcga tcgacaactt
     4201 cgacaagagc gtcgtgctct acgacaagac ggtcgccgag ggcaacacgc ctccgctcga
     4261 gctccgcgag gaatcgatcc agtacatcgc ggtcgcgttc accgatccgt ggctcggcga
     4321 gaccgatacc gatccatcga aggcattcga gcgcgccaag aagaagtacg cgggccgcga
     4381 gaacgagccg cacgtgcgtg acgtgtgggt cgcgatgggc caggcgttcg aggatctaca
     4441 ggcgtgggac caggcggtcg actcgtatcg catcgcgatc ggtccgccgt gggagttgaa
     4501 tccgaagaac ccgatcgttc accagaagat cgtgaacgcg ttcgagagca agggtgacaa
     4561 gttcgccgcc gacaaggccg cggccgagct cgcgacgcgc tatgcgccag gcacggcgtg
     4621 gtaccaggcg aacgagaagg accgcgaggc gatggataac cagcgccgga ttgcggagcg
     4681 cgcgctgtat gcggctgcgc gcaacacgca ttcggcggcg acgacgctgc gcaaggatta
     4741 cgatgcggcg ggcgcgaagg acccgaagca gaaggaggag tacctcgcga tgtacgggca
     4801 ggcagccgag ctgtaccgcc agttcatcaa cacgtacccc gagagcgact acatctacga
     4861 gttcaacttc ctgctcggcg agacgctgtt ctacagcgag aagtatcgag aggcgatcgt
     4921 ccagtacaag tgggtccgcg atcaccgcga cctcggccag acgtactacc tcgacgctgc
     4981 gcgctcggtg ctcgcatcgt acgaagcagc gaaggacaag gaagtcgccg agggcaagct
     5041 gcagccgctg aaggttccga cggtcgccga gctcaaggcg atcccgcagc cgtggcagcc
     5101 gcagccgatc ccgcagatct atctcgagct gcaggccgag tacgacaact accagaacat
     5161 cgtgcaggac ccggcggcgg cgcccggtca gggcatcaac gccgcactga tctcgctcgc
     5221 gtacttccac gtcgacgacg cgatcgcgcg cttcacgaag gtgatggaca agttctgcgc
     5281 ggcggcgccg atgaagaagg gcgagctcgc cccggctgct cgtgcgaagg acggcatcct
     5341 cgcgatctac gaggcgcagg cgaacttcga cgcgatcgag gcgacgaaca agcgattcat
     5401 cagcgcgaag tgtggtgacg ccaacgcgat ccagctcgcg atcacgcaga accgctcgct
     5461 caacttctct cgcgcggccg acctctacaa gaagcaggag tacatcccgg ctgccgaggc
     5521 gttctatcgc ttctacaaga cggcgccgca ggacgatccc gatctgccgg tcgcgctcta
     5581 caac
//
LOCUS       DASUFX010000825         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_690653,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000825 DASUFX010000000
VERSION     DASUFX010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690653"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFV99_33270"
     CDS             <1..322
                     /locus_tag="VFV99_33270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064309.1"
                     /translation="VGKKALPPADAIGEYIGLTRLGARGVAIAANTMDQVAQRYAGRE
                     EEPFQRAARYRNAYLTDLWQQLIDTGIQIEPILIDGQWREIDTGQDLERARELLESRP
                     KEWT"
     gene            319..879
                     /locus_tag="VFV99_33275"
     CDS             319..879
                     /locus_tag="VFV99_33275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064310.1"
                     /translation="MTRLASMFCLSVAVVAVVGLAGCSKPSEADCRKALANMQRLMGT
                     DNLRDDGRIEGDVRRCKGGSKKKAVACAIKATTLDELRSCDFFHVPLNFTPPNVGSAG
                     SAGSDAAGSAAGSAAGSTAAGSAAGSGSTAGSAGGSAAGSAAAGSAAGPGSASGSAAP
                     GGSAASPGAAAGSAATSAGSAVGSAK"
     gene            complement(901..1485)
                     /locus_tag="VFV99_33280"
     CDS             complement(901..1485)
                     /locus_tag="VFV99_33280"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5064311.1"
                     /translation="MPAAKLATYDDLLAFTDPEDKRAELIHGVIVERVGPSGQHSNTQ
                     GRLFGYLMRRFERSQGQRWPGGWWFGTECDMQYETHEVFCHDLIGWRRDRMAGRPEGR
                     PQRLRPDWACEIISPSTEKHDFIDKMPVLHRANVPYFWIVRPIEKTLLVHRWTPDGYV
                     VRNAAASGDVIRAEPFDAVELRVAVIFGDEDDEE"
     gene            1652..2572
                     /locus_tag="VFV99_33285"
     CDS             1652..2572
                     /locus_tag="VFV99_33285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064312.1"
                     /translation="MHAPLVIASVLASWTLASGIARADKPTLVALAPARDARKAVAIG
                     PAGQVYQPDGKGAWVRTQAGGVAEELVSASTIGTTVIAGAKGAPPFKLKGGAWTALNL
                     GLKVKATIGNGSRPLAIVGTSVFALDKRQPVKLPNVPTTVTSVMAAGASARGVVIATD
                     QGLLRLAGKIFKPLAKAPKTVRMLIDDRWALVDRGALDLKTMKPFVWPAGVQIVEATT
                     IGNDFVGVSMHGKTIELITIKAGKVARETVPIDNAKPVVGVVVDKQARVAIALRDGRI
                     ALREKGTWTVSEVREELAAPKPGPEPAESP"
     gene            2569..3645
                     /locus_tag="VFV99_33290"
     CDS             2569..3645
                     /locus_tag="VFV99_33290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5064313.1"
                     /translation="MTDKREKHLVSHFFNVAVLVVGGVALAWMLNKLGWDNVKRVLGG
                     VGGWFVVIVAFDVAALACEAAAIHQFMRPESRMIKYWRVLAAQASGRAINILTPGGAL
                     GEPTKVTMMVAHAPRGRVVSAIVMINLASFYLSVAILLVGVPITALLVDLPHDLKLVV
                     WIGLAVIIAVVTGIGVLIQRGALGTVLAAAQRARIVSAERAEKLKAKLANVDQRIREL
                     QADRSEGTRLGLMFVCAARLCAWVATTVVLYTVGVQLGFTVLCGVFSVGVLIGWISAF
                     VPFGLGVADGSNYALYNVLGAAGAHGVFVTLLGRARSLTLALLGLLVMAAGHASSRYE
                     VHRRNKLIARLEAEHGSESPLRSG"
     gene            3770..>4413
                     /locus_tag="VFV99_33295"
     CDS             3770..>4413
                     /locus_tag="VFV99_33295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064314.1"
                     /translation="MAPHVLVADDDAWILRMVATVLEKRGYSVETAVDGEDALNRALA
                     HTPDLLITDVMMPKMDGWSLVRQLRAHAELAMLPVIFLTALSSEDDRIRGFRLGADDY
                     VTKPFRFEELDLRVAKTLRRTAQTMQETRDQLAGSGLRGDLSQVGLSSLLVLIEMERK
                     TGLLQLRAPEAGLSAQILVREGKVVHARLDENDELVDAECVYYLLTWGAGEFEFV"
BASE COUNT          723 a         1437 c         1487 g          766 t
ORIGIN      
        1 tgttggcaag aaagcgctgc caccggcgga cgcgatcggc gaatacatcg ggctcacgcg
       61 cctcggtgcg cgcggcgtcg cgatcgccgc gaacacgatg gatcaggtcg cgcagcggta
      121 cgccggtcgc gaagaagagc cgttccagcg cgctgcgcgc tatcgcaacg cgtacctgac
      181 cgatctgtgg caacagctca tcgataccgg catccagatc gagccgatcc tgatcgatgg
      241 ccaatggcgc gagatcgata ccggccagga tctcgaacgc gcgcgcgagc tgttagagtc
      301 gcgccccaag gagtggacat gacacgcctc gcttcgatgt tctgtctgtc ggttgccgtc
      361 gtcgccgtcg ttggacttgc gggctgttcg aagccaagcg aggcagattg ccgtaaggcg
      421 ctcgcgaaca tgcagcgcct gatgggcacc gacaacttgc gtgatgacgg ccgcatcgag
      481 ggcgacgtcc gccgctgcaa gggcggctcg aagaagaagg ccgtcgcctg cgcgatcaag
      541 gcgacgacgc tcgacgagct acgcagctgc gacttcttcc acgtgccgct caacttcacg
      601 ccgccgaacg taggttccgc gggctccgcc ggctctgacg cagccggctc cgcagccggt
      661 tccgcagcag gctcgacggc ggcaggttcc gccgcaggct ccggttctac cgccggctcc
      721 gccgggggct cggcggctgg ttcggctgcc gcgggtagcg ccgcaggtcc cggctcggca
      781 tcgggctcgg cagcaccggg cggctctgcg gcatcgccgg gcgcggcggc aggctcggcc
      841 gcaaccagcg cgggctcggc agtcggctcg gcgaagtagt cgcctcgtag tagtagtcgc
      901 ctattcctcg tcgtcttcgt cgccgaagat cacggcgact cggagctcga ccgcatcgaa
      961 cggctcggcg cgaatgacat cgcccgacgc ggccgcgttg cgaacgacgt aaccatctgg
     1021 cgtccagcga tgcacgagca acgtcttctc gatcggccgc acgatccaga agtacggcac
     1081 gttggcacga tggagcaccg gcatcttgtc gatgaagtca tgtttctcgg tcgatggcga
     1141 gatgatctcg caagcccaat cgggacgtag acgctgtggt cggccttcgg gccgcccggc
     1201 catgcggtcg cgacgccatc cgatcagatc atggcaaaac acttcgtgcg tctcgtattg
     1261 catgtcgcac tcggtgccga accaccagcc accgggccaa cgctgtccct gcgagcgttc
     1321 gaaacgtcgc atcagatagc cgaagagacg cccctgcgtg ttgctgtgct gaccgctcgg
     1381 accaacgcgt tcgacgatca cgccatgtat cagttcggca cgcttgtctt cggggtcggt
     1441 gaacgcgagc agatcgtcgt acgtggcgag cttcgcggcg ggcatcggga tcatcatagc
     1501 ccgcgcgctc tgcacggcac acgccatcgt cgggcagccg tgaaccatgc gagatgactc
     1561 gccacgacgc gtgcgtgtcc gtccggcgaa ccggtagccg tccgcggctg gccgaatcgc
     1621 ggacgcgaac gcatcacgct atgctcgccg cgtgcatgcg ccgctcgtga tcgcaagcgt
     1681 gctcgcgagc tggacactcg cgagtggtat cgcgcgcgcg gacaagccga cgctcgttgc
     1741 gctcgcgcca gcgcgagatg cacgcaaggc ggtcgcgatc ggccccgccg gtcaggtcta
     1801 tcagcccgac ggcaagggcg cgtgggtgcg cacgcaggcc ggcggcgtcg ccgaggagct
     1861 cgtcagtgcg agcacgatcg gcaccaccgt gatcgccggt gcgaagggcg cgccgccgtt
     1921 caagctcaag ggcggtgcgt ggaccgcgct gaacctcggc ctcaaggtga aggccaccat
     1981 cggcaacggt tcgcgcccgc tcgcaatcgt cggcacgagt gtgttcgcgc tcgacaagcg
     2041 ccagccggtg aagctaccaa acgtgccgac gacggtgacg tcggtaatgg cagccggtgc
     2101 gtctgctcgc ggcgtcgtca tcgcgactga tcaaggcctc ctgcggctcg ccggcaagat
     2161 cttcaagccg ctcgcgaaag cgccgaagac cgtgcgcatg ctgatcgatg atcggtgggc
     2221 gctcgtcgat cgtggcgcgc tcgatttgaa gacgatgaag ccgttcgtct ggccggccgg
     2281 cgtgcagatt gtggaagcga caacaatcgg caacgacttt gtcggcgtat cgatgcacgg
     2341 caagacgatc gagctgatca cgatcaaagc cggcaaggtc gcgagagaga ctgtgccgat
     2401 cgacaacgcg aaaccggtgg tcggcgtggt cgtcgacaag caggcgcgcg tcgcgatcgc
     2461 gctgcgcgac ggtcgcattg cgctgcgcga gaagggcacg tggaccgtga gcgaggtgcg
     2521 cgaggagctc gcagcgccca agccgggtcc tgaaccagcg gaatcgccgt gaccgacaaa
     2581 cgcgagaagc atctcgtcag tcacttcttc aacgtggccg tgctggtcgt cggcggcgtc
     2641 gcgctcgcgt ggatgctgaa caagctcggc tgggacaacg tcaagcgcgt gctcggcggt
     2701 gtcggcggct ggttcgtcgt gatcgtcgca ttcgatgtcg ccgcgctcgc gtgcgaagcc
     2761 gctgcgatcc accagttcat gcgacccgag tcgcgcatga tcaagtactg gcgcgtgctc
     2821 gccgcccagg cgagtggtcg cgcgatcaac atcctcacgc cgggtggcgc actcggcgag
     2881 ccgacgaagg tcacgatgat ggtcgcgcac gcaccgcgag gtcgggtcgt ctcggcgatc
     2941 gtgatgatca atctcgcgtc gttctatcta tcggtcgcga tcttgctcgt cggcgtgccg
     3001 atcacggcgc tactcgtcga cttgccgcac gacctcaagc tcgtcgtctg gatcgggctc
     3061 gcggtgatca tcgcggtcgt gaccggcatc ggcgtgctca tccagcgcgg tgccctcggc
     3121 accgtcctcg ccgctgcgca gcgcgcgcgc atcgtgtcgg ccgagcgcgc cgagaagttg
     3181 aaagcgaagc tggccaatgt cgatcagcgg attcgcgagc tgcaggccga tcggagcgag
     3241 ggcacgcgcc tcgggctcat gttcgtgtgt gcggcgcggt tgtgtgcctg ggtggccacc
     3301 acggtcgtgc tctacaccgt cggcgtacag ctgggcttca cagttctgtg cggcgtgttc
     3361 tccgtgggcg tgttgatcgg ctggatctcg gcgttcgtgc cgttcgggct gggcgtcgcc
     3421 gatggcagca attacgcgct gtacaacgtg cttggcgccg ccggcgccca cggcgtgttc
     3481 gtgacgctgc tcggccgtgc gcgcagcctc acgctcgcct tgctcggcct gctcgtcatg
     3541 gctgccggcc acgcgtcgag ccgatacgag gtacaccgac gcaacaagct gatcgcacgg
     3601 ctcgaagccg agcacggctc cgagtcaccg ctgcgaagtg gctgatcgaa ggtccacgcg
     3661 tttcgtgcaa gtagtacaag tagtgcctga ccccgcgtta cggacgggtt agccggtgat
     3721 cttcgcaact tgccgcactg ccacgtagca cgctaccttg gggctcgcga tggctccgca
     3781 tgtcctggtg gctgatgacg acgcctggat tttgcgcatg gtcgcgaccg ttctcgagaa
     3841 gcgcggctac agcgtcgaga ccgcggtcga cggcgaggac gccctcaatc gcgctctcgc
     3901 ccacaccccg gatctgctga tcacggacgt gatgatgccg aagatggacg gctggtcgct
     3961 cgtccgtcag ctgcgcgcgc acgccgagct cgcgatgctg cccgtgatct ttttgaccgc
     4021 gctgtcctcc gaggacgatc gcatccgcgg gtttcggctc ggcgctgacg actacgtcac
     4081 gaagccgttt cgcttcgaag agctcgacct tcgcgtcgcc aagacgctgc gtcgcactgc
     4141 acaaacgatg caggagacac gcgatcaact cgcaggcagt ggtcttcgcg gtgacctctc
     4201 gcaggtcggc ctcagctcgc tgctcgtact gatcgagatg gagcgcaaga ccggcctctt
     4261 gcagttacgt gcacccgagg ctggcctgtc ggcgcagatc ctcgtgcgcg aaggcaaggt
     4321 cgtgcacgcg cggctcgacg agaacgacga gctcgtcgac gccgagtgcg tctattacct
     4381 gctgacctgg ggcgccggcg aattcgagtt cgt
//
LOCUS       DASUFX010000826        10591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_969591,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000826 DASUFX010000000
VERSION     DASUFX010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969591"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VFV99_33300"
     CDS             <1..1702
                     /locus_tag="VFV99_33300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064315.1"
                     /translation="VIAVSCAATFGCTDVMDDTGVAEQSLGGAPVGLAVEVDNGAGIA
                     LQLKRGQTYYLNQIDMRASITATTEEGVAGLRTQGDFASLPWLGIHQDDQEFILLSNA
                     DGTFTRRRFFRDALWMNLPSFYVLEQLDAKGHRIGESVFANAGVDAFRTEADDFFDRR
                     FRAIQWTKDCHTSTDCTGATNFEEEALVELRYGKHPTRTFTLAPTTASLRLTWSLRPG
                     HPYDIPVTQIDNPPFAYGFNIAVHPVTAPRADGTYAPGSDITFKVQLQDGQGNPLHGD
                     GALPSYNDVEFGANPAGIQYYRAFFDPTTTYWRRKHRERMMMAEFLGPAQNAQPIRTI
                     IDLDKFLNEDDVQVTGTLEVDGVHSEFHTFPTARDLFGGAFDPVHAGWANPVPDTWTH
                     HIPENAPAGTYFMNMKGRRTYMGEDVPFTRTLEIQVGTPTRTTATLNTGPCASCHTGE
                     SALNKVLHANDNRGTCAGCHVPLGFELEGPIYVRTHFIHSRSRRFDAPLDKCASCHLT
                     KESIQRTSKSACLSCHKSYPADHVQAYGPVESMYVGGGRESFQQCTTTCHLTHPNSQL
                     "
     gene            1736..2881
                     /locus_tag="VFV99_33305"
     CDS             1736..2881
                     /locus_tag="VFV99_33305"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX5064316.1"
                     /translation="MAHARTSERDPKKAAEELCNQLGSTKPKLVVMFASRDRDQRALN
                     VAVRERLPSGTRLIGATTGGEIDREGVHDGTVVMAALSGDLEVGLGMGHKMSIDAAAA
                     GETAIRTAARELGARADDLGTRKYGALVIDDGFAYRKEELLLGVMSMNQALTAVGGGA
                     SDSEPDVAKQSAELHVDGEVSSDAAIVALFRTDAPWAALRSHWYAPTGQTLRITKIDN
                     TAKRALEIDGKPAAQRYADLLGVGVNDLEFGTPNGFAVRPTALRVGREYFIRAPWKPL
                     EDGSILFANMLEEGSELELVKITDIVDSTKRFFEQELPSRVPSARAALLFHCSGRKWF
                     AQSTGKYPALGDTFKAAPPCIGMNVHFEIYCGFHINTTLTSLVFGAS"
     gene            2881..4974
                     /locus_tag="VFV99_33310"
     CDS             2881..4974
                     /locus_tag="VFV99_33310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064317.1"
                     /translation="MTVDAERRVEELLAENRRLRAEIEVKEQHAARAMASFQQRALLM
                     EITRQQNEDLDRLAAELARAKKVEEERAREIEAAARLKSEFLANFSHEIRTPLNAITG
                     YCDLLIRDEGSRLTPHGRRDLSVIKANAKTLLALINDILDLSKIEAGRAEVVKEVVDL
                     VELADECTASVREILKGKDVTLNADIGPRQVFTDPLKLRQILLNLLSNAAKFTDAGEI
                     VLTAEAQGTTLMISVEDTGAGIPDDQLPFIFDKFRQVDGSARRRVGGTGLGLAIVKEV
                     VQILGGTLNATSTLGRGSKFTLALPGAIDTSKVSRPSPTGTGVVPAQTNGPFTVLIID
                     DDPMVQHLLRGHLESEEFRVVSAQDGVEGLTLAREVRPTVIILDIHLPRLDGWTVLGE
                     IKGDPALASIPVIMMSVEEQRARGFSFGACEYLVKPVEPDRLVTVVKRALLPSDGDVL
                     VVDDDESTRELVSRHLRRAGYSTVEARDGEEALLRARVISPGLVILDLLMPGVDGFEV
                     LRTMRAEGMRTPVVVLTGKELASDEERILRDGLARVVQKGGMALESVVREAKQLLLAK
                     RVVESGRLPRILYVEDSPQNRDIVRRYLADDYEVIEAEDGEHGIERVLRDAPDLILMD
                     LSLPRLDGWEATRRLKANGTVKHIPVIAVTAHAAREDQTRAYQAGCCDYITKPIDREV
                     LMTSIRKHLQKSKST"
     gene            4971..5342
                     /locus_tag="VFV99_33315"
     CDS             4971..5342
                     /locus_tag="VFV99_33315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064318.1"
                     /translation="MTTTLRVLVVDDDPLQLELVDRALSRDGFELRGITSCERLVAAM
                     GDFKPEVVLLDVNMPDTPPERVIALVREAASNARIVLYSAWEDSKLRALANGLGADAY
                     ISKSESVFSIGGRIRDLFRGN"
     gene            5381..7315
                     /locus_tag="VFV99_33320"
     CDS             5381..7315
                     /locus_tag="VFV99_33320"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5064319.1"
                     /translation="MLEVPIYNKTLRKEVGRGELDYEVYLRTRELLALQTPANELVVP
                     DELLFQVMHQTQELWLKCVAFETANVVEHLDGDSVFAALDALDRIAQITRVLAEQIRV
                     MFTLSPQRFQVIRRSLGNGSGLESPGYNQLLTAAAAAFDAFKRIVERRNATLLAIYEA
                     PEAYAELHRIAERFVDWDGNVQTWLVEHFMLVRRTIGIDKGVRALDGFPTVALGSRMT
                     KPLFAELWNLRVEMTRAWKREGGFLPGEQREDLRVEPVASRTGTAEVEPFAIGSLRIE
                     FPVLARCVYLNSNATGATPRAAKTALDSYWRTLENWRDEVWERWWRDLHAHADDLAEL
                     IRAPQGSVVCDTNLATLFSRVMSAFGYGERPNVVTTDLEFPSIPFTIRAFERYGAVAI
                     VVPSRDGATIDTEAVVRAIDERTQLVCLSHATFTTGAVTDLEPIVRRAHEVGALVVLD
                     AYQSVGTMPIDVDELGVDFLLGGAHKWLCGSYESAFLYVRPSLLQRLRPAATGWIAGA
                     DPLSFAPQTEWADTARRFAGGTPAMLPTLVSRPGLAMVRGVGLETIRALSLARTDRII
                     ARADDAGLAVATPRSYARRGGIVALRFPGDAEIARALIASGFVCSYRAGLRIAPHFYN
                     TDEEIDRFMDELVRRAREAL"
     gene            7312..8307
                     /locus_tag="VFV99_33325"
     CDS             7312..8307
                     /locus_tag="VFV99_33325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5064320.1"
                     /translation="MNARSLLSLLHHADKALELVRTARDLGIAARLDTGPVSLRVLAD
                     ETKTKPLRLYKFLDGLESLGLVTRRQPTDELLASEYVSNEPLVAAIEAVLGEQSIERD
                     RDRYPWREIHGRLPEVLAGKLDARFAWPPGTADEIRDFERAMAAGCPPIIEAFLSAQN
                     RVFDGAKRWLDVGGGDGTVAAAVLRERSTLVGDVFNLADVAPLVAARARAAEVEPRLG
                     FVAGDFLAGPLPRGYDVISFVRVLHDWPADVARSLLVNARDALAPGGRLVICEEFRDR
                     DRLAVQFFWTYFLIGVDACVSRLRDLEWYVEALTRLDCGVELVPGAFDLVIATRR"
     gene            complement(8304..9416)
                     /locus_tag="VFV99_33330"
     CDS             complement(8304..9416)
                     /locus_tag="VFV99_33330"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX5064321.1"
                     /translation="MRALLLLCLVACTANVDIVGDPEEDSAAGAGLTEADTQGADADR
                     SFIEARVCASGATTFGIDVSYHNGTINWTSVKNAGVKFAIIRVSDGDVFQDPKFSSYW
                     ASAKQVGIIRGAYQFFRPSQNVTAQADKMIAAYGGSYTPGDLPPVIDVEADGGLAPAT
                     VAARIRTWVDRVKNALGVAPIVYTGKYFWRDEVGGPTSFVNNPLWIAQYTSLCPDLPA
                     PWQRWTFWQYSESGSVAGISGDVDLDRFNGSVADLLAFANSSTMPPPPSGGCTSATLN
                     KDVPELTCVQAASDQKWYRCEGGAWIAKTSTTGCASTYGWCSSATLGRSVAPRSCVQA
                     ASDDKWYQCDGHGWVQPVDPIAETGPAGLCSASYQL"
     gene            9709..>10591
                     /locus_tag="VFV99_33335"
     CDS             9709..>10591
                     /locus_tag="VFV99_33335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064322.1"
                     /translation="MSASTTEIPSSIDSDLEPGTVVGEYRVEGRLGQGAFGTVYKGVH
                     PLIGKVVAIKVLARKYSVDPEMVSRFVAEARAVNQIRNRHIIDIFSFGQLEDERQYYV
                     MEFLEGEPLDARLERVGNLSIADALPILRAIAKALDAAHAKGITHRDLKAENVFLGTD
                     SEGALFPKLLDFGIAKLLSADDGIKHKTRTGIAVGTPYYMSPEQCRGKDVDHRTDIYA
                     FGVLMYRVLTGQYPFDADDPMSILMKHFNDDAPPPSTHLPDLPSTIDDVVLWMLAKDP
                     ADRPADCRTAVHALEDAA"
BASE COUNT         1864 a         3421 c         3457 g         1849 t
ORIGIN      
        1 ggtgatcgcg gtctcgtgtg cagcgacatt tggctgcacg gacgtcatgg atgacaccgg
       61 ggtcgccgag cagtcgctcg gtggcgcgcc cgttggcctc gcagtcgagg tggacaacgg
      121 tgcgggaatc gcgcttcagc tgaagcgcgg gcaaacgtac tacctgaacc agatcgatat
      181 gcgtgcatcg atcacggcga ccaccgaaga aggcgtcgcc ggactgcgca cgcagggtga
      241 tttcgcgagc ctgccgtggc tcggcattca ccaggacgac caggagttca ttctgctatc
      301 caatgccgac ggcacgttca cgcgccgtcg tttcttccgc gacgcgctgt ggatgaatct
      361 gccgagcttc tacgtgctcg agcagctcga cgcgaaggga catcgtatcg gcgaaagcgt
      421 cttcgcgaac gccggtgtcg acgcgttccg caccgaggcc gacgacttct tcgatcgccg
      481 gttccgcgcg atccagtgga cgaaggactg ccacacgagc acggattgca ccggtgcgac
      541 caacttcgag gaggaagcac tcgtcgagct ccgctatggc aagcatccga cgcgcacgtt
      601 cacgctcgca ccgacgaccg cgtcgctgcg gctgacctgg tcgctgcgcc cgggccaccc
      661 gtacgacatt ccggtcaccc agatcgacaa tccgccgttc gcgtacggct tcaacatcgc
      721 tgttcacccg gtcaccgcac cacgcgccga cggcacgtac gcgccgggca gcgacattac
      781 gttcaaggtc cagctgcagg acggccaggg caacccgttg cacggcgacg gcgcgctgcc
      841 ttcctacaac gatgtcgagt tcggcgcgaa cccggccggc atccagtact accgcgcgtt
      901 cttcgatccg acgacgacgt attggcgccg caagcatcgc gaacgcatga tgatggccga
      961 gttcctcggc ccggcccaga acgcacagcc gatccgcacg atcatcgacc tcgacaagtt
     1021 cctcaacgag gatgacgtcc aggtcaccgg cacactcgaa gtggacggcg tgcactccga
     1081 gttccacacg ttcccgacgg cgcgcgatct gttcggcggc gcgttcgacc cggtgcacgc
     1141 gggctgggcc aacccggtgc ctgacacgtg gacccaccac attccggaga atgcgcctgc
     1201 gggcacgtac ttcatgaaca tgaagggccg ccgcacgtac atgggcgagg acgtcccgtt
     1261 cacgcgcacc ctcgagatcc aggtcggtac cccgacgcgt acgaccgcga cgctgaacac
     1321 tggcccgtgc gcgagctgtc acaccggcga gtcggcgctc aacaaggtcc tccatgcgaa
     1381 cgacaaccgt ggcacctgcg cgggctgtca cgtgccgctc ggcttcgagc tcgagggccc
     1441 gatctacgtg cgcacgcact tcattcactc gcgctcgcgc cgcttcgacg cgccactcga
     1501 taagtgcgcg tcgtgtcacc tcacgaagga gagcatccag cgcacgagca agtcggcctg
     1561 tctgtcctgt cacaagtcgt acccggccga ccacgtccaa gcctatggcc ccgtcgagag
     1621 catgtacgtc ggcggtgggc gcgaatcatt ccagcagtgc acgacgacct gtcacctcac
     1681 gcacccgaat agccaactct gatcaaggag cgacggaacg atgcctagtg ttgatatggc
     1741 ccatgcccgc acgagcgaac gcgatcccaa gaaagcggcc gaggaattat gcaatcagct
     1801 cggcagtacg aagccgaagc tcgtcgtgat gttcgcctcg cgcgatcgcg atcagcgagc
     1861 gctcaacgtc gcggttcgcg aacgcttgcc gtcgggcacg cgcctgatcg gtgcgacgac
     1921 cggcggtgaa atcgatcgtg agggggtgca cgacggcacg gtcgtcatgg ccgcgctgtc
     1981 cggtgatctc gaggtgggtc tgggcatggg ccacaagatg tcgatcgatg ccgcggcggc
     2041 gggcgagacc gccattcgaa ccgccgcgcg cgagctaggt gcacgtgccg acgacctcgg
     2101 tacgcgcaag tacggcgcgc tcgtgatcga cgacggcttc gcataccgca aggaggagct
     2161 gctgctcggc gtgatgtcga tgaaccaggc attgaccgcc gtcggcggcg gcgcgtccga
     2221 cagcgagccc gacgtggcga agcagtcggc cgagctgcac gtcgacggtg aggtcagctc
     2281 ggacgcggcg atcgtcgcgc tgttccgcac cgacgcaccg tgggccgcgc tgcggtcgca
     2341 ctggtatgcg ccgaccgggc aaacgctgcg catcacgaag atcgacaaca cggcaaagcg
     2401 tgccctcgag atcgatggca agccggccgc ccagcgttat gccgatctgc tcggcgtcgg
     2461 cgtcaatgac ctcgagttcg gcacgccgaa tggtttcgcg gtgcgcccga ctgcattgcg
     2521 cgtcggccgc gagtacttca tccgtgcgcc gtggaagccg ctcgaggacg gctcgatctt
     2581 gttcgcgaac atgctcgagg agggctccga gctcgagctc gtgaagatca ccgacatcgt
     2641 cgacagcacg aagcgcttct tcgagcagga gttgccatcg cgtgtcccgt cagcacgcgc
     2701 ggcgctgctg ttccattgca gcggtcgcaa gtggttcgcg cagagtaccg gaaaatatcc
     2761 ggcgctcggt gacacgttca aagccgcacc gccgtgcatc ggcatgaacg ttcacttcga
     2821 gatctactgc ggcttccaca tcaacaccac gctgacctca ctcgtcttcg gagcctccta
     2881 atgacagtgg acgccgaacg gcgcgtcgaa gagctgcttg ccgagaaccg tcgactgcgt
     2941 gctgaaatcg aagtcaaaga gcagcacgca gcgcgcgcga tggcgagctt tcagcagcgc
     3001 gcgttgttga tggagatcac gcgccagcag aacgaagatc tcgaccgcct tgccgccgag
     3061 ctcgctcggg cgaagaaggt cgaggaggaa cgtgcgcgcg agatcgaagc ggccgcgcgg
     3121 ctcaagagcg agttcctcgc gaacttctcg cacgagatcc gcacgccgct gaacgcgatc
     3181 accggctact gcgatctgct gattcgcgat gaaggctcgc ggttgacgcc acacgggcgg
     3241 cgcgacctgt cggtgatcaa ggcgaacgct aagacgttgc tcgcgctgat caacgacatc
     3301 ctcgatctat ccaagatcga agccggccgt gccgaggtcg tgaaggaggt cgtagacctc
     3361 gtcgagctcg cggacgagtg cacggcaagc gtgcgcgaga tcctcaaggg caaggacgtc
     3421 accctcaacg ccgacatcgg gccgcggcag gtcttcacgg acccgctcaa gctgcgccag
     3481 atcctgctca acctgttgtc gaacgccgcg aagttcaccg acgcgggcga gatcgtgctg
     3541 acggcagagg cgcagggcac gacgctgatg atctcggtcg aagacaccgg tgctggcatt
     3601 cccgacgatc agctgccgtt catcttcgac aagttccggc aggtcgatgg atccgcacgc
     3661 aggcgcgtcg gcggaaccgg gctcgggctc gcgatcgtca aggaagtggt ccagattctc
     3721 ggcggcacac tgaacgcgac gagcacgcta ggtcgaggtt cgaagttcac gctcgcactg
     3781 ccgggcgcga tcgatacgtc gaaggtttcg cggccatcgc ctacaggtac cggtgtcgtg
     3841 ccagcgcaga cgaacggccc attcacggtc ctcatcatcg acgacgatcc gatggtccag
     3901 cacttgctgc gtggccatct cgagtccgag gagttccgcg tggtctccgc gcaggacggc
     3961 gtcgagggcc tgacgctcgc gcgcgaggtc aggccgaccg tgatcatcct cgatattcac
     4021 ctgccgcggc tcgacggctg gacagtgctc ggcgagatca agggcgatcc agcgctcgct
     4081 tcgatcccgg tgatcatgat gtcggtcgag gagcagcgag cgcgcggctt ctcgttcggc
     4141 gcgtgcgaat acctcgtgaa gccggtcgaa cccgaccggc tggttaccgt cgtcaagcgt
     4201 gcgctgctgc cgtccgacgg tgacgtgctc gtcgtcgacg acgatgaatc gacgcgcgag
     4261 ctcgtctcgc ggcatctccg ccgcgcgggc tattcgacgg tcgaggcacg tgatggcgag
     4321 gaagccttgc ttcgcgcacg cgtgatctcg ccaggtctcg tgatcctcga tctgctgatg
     4381 ccgggcgtcg acgggttcga agtcttacgc acgatgcgtg cggagggtat gcgaacgccg
     4441 gtcgtcgtcc tcaccggcaa ggaattagcc tcggacgaag aacgaatatt acgagacggc
     4501 ctcgcacgtg tcgtgcagaa gggaggtatg gcgctcgaaa gcgtcgtgcg ggaggcgaag
     4561 cagctgttgc tcgccaagcg cgtcgtcgag agcggacgcc tgccgcgcat tctgtacgtc
     4621 gaggactcgc cgcagaaccg cgacatcgtg cggcgctatc tcgccgacga ctacgaggtc
     4681 atcgaagcgg aggatggcga gcacgggatc gagcgggtgc tgcgagacgc gcccgacctg
     4741 attctcatgg atctctcgct gccgcggctc gacggttggg aagccacccg tcggctcaag
     4801 gcgaatggaa ccgtaaagca catcccggtg atcgcggtga cggcgcacgc tgcacgtgag
     4861 gaccagaccc gggcgtacca ggccgggtgt tgcgactaca taacgaagcc gatcgaccgc
     4921 gaggtgttga tgacatcgat caggaagcat ttgcagaaga gcaagagcac atgacgacga
     4981 cgctacgtgt ccttgttgtc gacgacgatc cgctccagct cgagcttgtc gatcgcgctc
     5041 tgtcgcgcga cggcttcgag ttgcgtggga tcacgtcgtg cgagcgactc gtggcggcga
     5101 tgggtgactt caagcccgag gtcgtgttgc tcgacgtcaa catgccggat acgccgcctg
     5161 agcgcgtgat cgcgctcgtt cgagaagcgg cgagtaatgc acggatcgtc ctctactcgg
     5221 catgggagga ctccaagtta cgcgcgctcg caaatgggct gggcgcggac gcgtatatct
     5281 cgaagagcga gtcagtgttc tcgatcggcg ggcgcatacg cgatttgttt cgggggaact
     5341 gaaaatgaat cgaagcgaag cagcggcggc gttgcgcagg gtgttagagg ttccgattta
     5401 caacaagacg ctccgcaagg aagtcggacg cggtgagctg gactacgagg tctatctgcg
     5461 aacgcgcgag cttctcgcgt tgcagacgcc agctaacgag ctcgtcgttc cggacgaact
     5521 gctgttccag gtgatgcacc agacgcagga gctgtggctc aagtgtgtcg cgttcgagac
     5581 cgcgaatgtc gtcgaacatc tcgacggaga ctcggtattc gccgcgctcg atgcgctcga
     5641 tcgcatcgcg cagatcacgc gcgtgcttgc cgagcagatc cgcgtgatgt tcacactgtc
     5701 gccacagcgg tttcaggtga tccggcgcag cctcggtaac ggtagcggtc tcgagtcgcc
     5761 gggctacaac cagctactca cggcggctgc cgcagcgttc gacgcgttca agcgcatcgt
     5821 cgagcgtcgc aacgcgacat tgcttgccat ctacgaggcg cccgaggcgt atgcagagct
     5881 tcatcgcatc gccgagcgat tcgtcgactg ggacggcaac gtgcagacct ggctcgtcga
     5941 gcacttcatg ctcgtgcgcc gcacgatcgg catcgacaag ggagtccgtg cgctcgacgg
     6001 gttcccgacg gtggcgctcg gttcgcgcat gacgaagccg ctgttcgccg agctatggaa
     6061 tctgcgcgtc gagatgacgc gtgcgtggaa gcgcgagggc gggttcctgc cgggcgagca
     6121 gcgcgaggac ctgcgtgtcg agccggtggc atcgcgaacc ggaaccgcgg aagtcgagcc
     6181 gttcgcgatc ggatcgctgc gcatcgagtt cccggtcctg gcgcgctgcg tgtacttgaa
     6241 cagcaatgcg accggcgcga cgccgcgtgc agcgaagact gcgctcgaca gctactggcg
     6301 cacgctcgaa aattggcgcg acgaagtatg ggagcgctgg tggcgcgacc ttcacgcgca
     6361 cgccgacgac ctagccgagc tgatccgagc gcctcaggga tcggtcgtgt gcgacacgaa
     6421 cctcgcgacg ctgttctcgc gcgtgatgag cgcgttcggc tatggcgagc gcccgaacgt
     6481 cgtgacgacg gacctcgagt ttccgtcgat cccgttcacg attcgcgcat tcgagcggta
     6541 cggcgcggtc gcgatcgtcg tgccatcacg cgacggggcg acgatcgaca ccgaggctgt
     6601 cgtgcgcgcg atcgacgagc ggacgcagct cgtctgtctc tcccacgcga cgttcacgac
     6661 cggcgccgtc accgacctcg agccgatcgt acggcgcgca cacgaggtcg gcgcgctcgt
     6721 cgtgctcgac gcgtatcaat cggtcggcac gatgccgatc gatgtcgacg agctcggtgt
     6781 cgacttcttg ctcggcggcg cacacaagtg gctatgcggt tcgtacgaga gcgcgttctt
     6841 gtatgtgcgt ccgtcgctat tgcagcggtt gcggccggcg gcgactggct ggatcgcagg
     6901 tgcggatccg ttgtcgttcg cgccgcaaac cgagtgggcg gacactgccc ggcgattcgc
     6961 gggtggcacg ccggcgatgc taccgacgct cgtgtcacgg ccgggtctcg caatggtgcg
     7021 cggcgtcggg ctggagacga tccgggcgct gtcactcgcg cgaaccgacc gcatcatcgc
     7081 gcgtgcggat gacgccgggc tcgcagtcgc aacgccgcgt tcgtatgcgc ggcgcggtgg
     7141 catcgtcgcg ctgcggttcc ccggtgatgc cgagatcgcc cgggcgctca tcgcgtccgg
     7201 gttcgtctgt agttaccgcg ccggccttcg catcgcgcca cacttctaca acaccgacga
     7261 ggaaatcgat cggttcatgg acgagctcgt tcgccgggcg cgcgaggcac tgtgaacgca
     7321 cgctcgctgc tcagtctgct ccatcacgcc gacaaggcgc tcgagctcgt gcgcaccgca
     7381 cgcgatcttg gtatcgcagc acgcctcgat accggcccgg tgtcattgcg cgtgctcgcc
     7441 gatgagacga agacaaagcc gctccgcctc tacaagttcc tcgacgggct cgagagcctc
     7501 ggtctcgtga ctcgccggca accgacggac gagttgctcg cgagcgagta tgtatccaac
     7561 gagccactcg tcgcggcgat cgaggcggtt ctcggggagc agtccatcga gcgcgaccgc
     7621 gatcgctacc cgtggcggga gatccatggc cggctgcccg aggtccttgc cggcaagctc
     7681 gacgctcgtt tcgcgtggcc tccgggcacc gccgacgaga tccgcgattt cgagcgtgcg
     7741 atggcggctg gctgcccgcc gatcatcgag gcgtttctca gcgcgcagaa ccgagtgttc
     7801 gacggggcca agcgttggct cgacgtcggt ggtggtgatg gcaccgtggc ggccgcggtg
     7861 cttcgcgagc gcagcacact cgtcggtgac gtgttcaacc ttgccgacgt cgcgccactc
     7921 gtcgccgcac gtgcacgtgc cgctgaggtc gagccgcgct tgggtttcgt cgccggcgac
     7981 tttctcgccg gtccgctgcc gcgcggctac gacgtgatct cgttcgtgcg ggtgctgcac
     8041 gactggcccg ccgatgtcgc gcgctcgctg ctcgtcaacg cgcgcgacgc gctagcgccc
     8101 ggcgggcgac tcgtcatctg cgaggagttt cgtgatcgcg atcggctcgc ggttcagttc
     8161 ttctggacgt atttcctgat cggcgtcgac gcctgcgtga gccggttgcg cgatctcgag
     8221 tggtacgtcg aggcgctcac gcggctcgac tgcggggtcg agctggtccc cggtgcgttc
     8281 gacctcgtga tcgcgacgcg gcgctagagc tgatagctcg cggagcacaa tccggccggt
     8341 cccgtctcgg cgattggatc gaccggctgc acccagccgt gaccgtcgca ctggtaccac
     8401 ttgtcgtcgc tcgcggcttg cacgcacgag cgcggcgcca ccgagcggcc gagtgtcgcg
     8461 gacgagcacc agccgtaggt cgaggcgcag ccggtcgtcg acgtcttcgc gatccacgcg
     8521 ccgccttcgc agcggtacca cttctggtcg ctagcagcct gcacgcacgt gagctcgggc
     8581 acgtccttgt tgagcgttgc ggacgtgcag ccacccgatg gcggcggagg catcgtcgag
     8641 ctattggcga acgcgagcag atcggcgacc gatccgttga agcggtcgag gtcgacgtca
     8701 cccgagatgc ccgccaccga gccgctctcc gagtactgcc agaacgtcca gcgctgccac
     8761 ggcgccggca gatccgggca cagcgacgtg tactgcgcga tccacagtgg attgttcacg
     8821 aacgacgtcg ggccgccgac ctcatcgcgc cagaagtact tgcccgtgta gacgatcggc
     8881 gcgacgccga gcgcgttctt cacgcgatcg acccacgtgc gaatgcgcgc tgcgaccgtc
     8941 gctggtgcga gcccgccatc tgcttcgacg tcgatcaccg gcggtagatc gccgggcgta
     9001 taggagccgc cgtacgcggc gatcatcttg tcggcctgcg cggtcacgtt ctggctcggc
     9061 cggaagaact gatacgcgcc acgaatgatg ccgacctgtt tcgcgctggc ccagtacgag
     9121 ctgaacttcg ggtcctggaa cacgtcgccg tcggagacgc ggatgatcgc gaacttcacg
     9181 cccgcgttct tcacgctcgt ccagttgatg gtgccgttgt gatacgagac atcgattccg
     9241 aacgtcgtcg cgccgctcgc acacacgcgc gcttcgatga acgaacgatc ggcgtcggcg
     9301 ccttgtgtat ccgcttcggt gagaccagct ccagcggccg aatcctcctc gggatcacca
     9361 acgatgtcga cgttcgcagt gcaggcgaca agacacagca gtagcagtgc tcgcacgggg
     9421 cagcctccct gccgttcact tcagcaagcg atatgcctca tgacgttcac cgtgcgttct
     9481 gcacagcgtg ggaagcgcgt ttcacttcac gtgaacgtcg tcgggaagcc agcgcacgag
     9541 gtctcgacat cccactggga atcgattccg cacacgcttg tccgcgcttc ggctcggttg
     9601 caggagtgca tgtcgtacct ggtgggattc acacgcagta atcaatgatt cgcgatcgtg
     9661 ggatcctgct acggccaacc ttttgcacaa gcgggatagg gaccacgtat gtccgccagc
     9721 acgacggaaa ttccatcttc gatcgactcc gatctcgagc ccggaaccgt cgttggcgag
     9781 taccgcgtcg aaggcagact cgggcaaggt gcgttcggca ccgtctacaa gggcgtccac
     9841 ccgctgatcg gcaaggtcgt cgcgatcaag gtgctcgccc gcaagtactc ggtcgacccc
     9901 gagatggtgt cgcgctttgt cgccgaggcg cgtgccgtca accagatccg caaccgccac
     9961 atcatcgata tcttctcgtt cggccagctc gaggacgagc gccagtacta cgtgatggag
    10021 ttcctcgaag gcgagccgct cgatgctcgc ctcgagcgcg tcggaaacct gtcgatcgcc
    10081 gatgcgctgc cgatcctgcg cgcgatcgcg aaggcgctcg atgctgccca cgcgaagggc
    10141 atcacgcacc gcgacctcaa agccgagaac gtattcctcg gcaccgattc ggaaggtgcg
    10201 ctgtttccga agctgctcga cttcggcatc gcgaagttgc tgtcggccga tgacggcatc
    10261 aagcacaaga cgcgcaccgg tatcgcggtc ggcacgccgt attacatgtc gcccgagcag
    10321 tgccgcggca aggacgtcga tcaccgcacc gacatctacg cgttcggcgt cctgatgtac
    10381 cgggtgctga cggggcagta cccgttcgac gccgacgatc cgatgtcgat cttgatgaag
    10441 cacttcaacg acgacgctcc accgccgtcg acacatttgc ccgatctgcc gtcgacgatc
    10501 gatgacgtcg tgctgtggat gctcgcgaag gatccagcgg atcgtcctgc cgactgccgc
    10561 accgcggtgc acgcactcga ggatgcggcc g
//
LOCUS       DASUFX010000827         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1803466, whole genome shotgun sequence.
ACCESSION   DASUFX010000827 DASUFX010000000
VERSION     DASUFX010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803466"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFV99_33340"
     CDS             <1..251
                     /locus_tag="VFV99_33340"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2277 family protein"
                     /protein_id="HEX5064323.1"
                     /translation="PPTTPEEIHAAALQYVRKVSGMREPGKDALAAFERAVAQVTAAT
                     AKLLEKLPEHGEPRTREGEKEKAKLRWARREQAIRDRY"
     gene            complement(591..1304)
                     /locus_tag="VFV99_33345"
     CDS             complement(591..1304)
                     /locus_tag="VFV99_33345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX5064324.1"
                     /translation="MPRRHHRDRHHRETHRSARAGWLRAAVLGSNDAIVSTASLMMGV
                     AAANAPKDAILVAGIAGLVAGAMSMAAGEYVSVSSQLDAEHADIALEEEELAKAPEAE
                     LHELANIYINRGLDKHLAMQVAEKLSEHDRLGAHLRDELGISEQTRARPLQAAWISAL
                     SFATFALLPVLALLVAPASLRMETIGGVTLVSLASLGALGGYLGGAPKVRASLRVTIG
                     GALAMAVTAGIGRLLGLSL"
     gene            complement(1321..2145)
                     /locus_tag="VFV99_33350"
     CDS             complement(1321..2145)
                     /locus_tag="VFV99_33350"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5064325.1"
                     /translation="MGEITLAVVPSTTPGDARLALDSLCIALTKLLDTPVHGINCASY
                     SELALELEKDRVDYAWMSPTLMILTNENIQLRPLLTAVRDDRTDYRSVWFVDGKKPFR
                     SLEDVQNGVVAWVDRSSAAGYIVPRVHLAARGFDAAHHFRQELFLRSHAEVVRAVLDG
                     RADVGATYGQRPDEGQPVVRAGFLHVAPERHVRTLEWTQPIPNDVIVGHGLLSKPEHR
                     GFSNAILTLAERDDASRRVLFNVFHAEQFMTPSRNVLKPAQDLVTLAREHGLMSQL"
     gene            2220..>2567
                     /locus_tag="VFV99_33355"
     CDS             2220..>2567
                     /locus_tag="VFV99_33355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5064326.1"
                     /translation="MIGFAANSLLCRAALRGGAIDAASFTAVRLVTGAAVLVLIVAAR
                     KDSVRGEGTWLSAFALAGYAVAFSFAYLEIGAATGALLLFGAVQLTMIAGGLIRGERP
                     TPRQWLGWLVALSG"
BASE COUNT          457 a          835 c          873 g          402 t
ORIGIN      
        1 agccgccgac cacgcctgag gagattcacg cggccgcgct ccagtacgtg cggaaggtga
       61 gcggcatgcg cgagccgggg aaggacgcgc tcgcagcgtt cgagcgcgcg gtcgcacagg
      121 tcactgccgc gacggcgaag ctgctcgaaa aactgcccga gcatggcgag ccgcgcacgc
      181 gcgaaggtga gaaggaaaaa gcgaagctgc gctgggcgcg ccgcgagcaa gcgatccgcg
      241 atcggtacta agaaggatcc gacgtgcacg tgcacgtgca cgtgtcacgt gtcacgtgca
      301 cggaggctgt ggaccagttg ccctcagcgt cgacccgaac acgcgcaaga atgtgtttga
      361 gcacgggcga gttgcgacgc aacgtgagtg agcacgagcg acgttgcgat gcaacgtgaa
      421 cgatcacatg cgacgctgcg actcgacgtg ttccggcaac cacgatccaa cgttagatgg
      481 ttgcgcgaag cgtaaacctg attcgcgcca gagcacgagc acgagcacga gcacgagcac
      541 gtgcacgtga cacgagcaag tgtagggatc agaacgcgcg ggcctcgggc ttagagcgaa
      601 agcccgagca aacggccgat gcccgccgtg accgccatcg cgagcgcgcc gccgatcgtc
      661 acgcgcagcg acgcgcgaac cttcggcgca ccgccgagat agccgccgag cgcgccgagc
      721 gacgcgagac tgacgagcgt gacgccaccg atcgtttcca tgcgcagcga tgccggggcg
      781 acgagcaacg cgagcactgg cagcagtgca aacgtcgcga agctgagcgc cgagatccac
      841 gccgcctgca gtgggcgcgc acgtgtctgc tcggagatgc cgagctcgtc gcgcaggtga
      901 gcaccgaggc gatcgtgctc gctgagcttc tcggcgacct gcatcgcgag gtgcttgtcg
      961 agaccgcggt tgatgtagat gttggccagc tcgtgcagct cggcctcggg tgccttcgcg
     1021 agctcttcct cctcgagcgc gatgtcggcg tgctcggcat ctagctgcga gctcaccgat
     1081 acgtactcgc ccgcagccat cgacatcgca cctgcgacca agccggcgat gccggcgacg
     1141 agaatcgcat ccttcggcgc attggccgcc gcgacgccca tcatcaagct cgccgtcgac
     1201 acgatcgcgt cgttcgagcc gagcacggcc gctcgcagcc accctgcacg tgcgctccgg
     1261 tgcgtctcgc gatggtgacg gtcgcgatgg tgacggcgag gcataccccg gcagtctagt
     1321 tcaaagctgc gacatcagcc cgtgctcgcg cgcgagcgtg acgagatcct gcgccggctt
     1381 cagcacgttg cgcgacgggg tcatgaactg ctcggcgtga aatacgttga acagcacgcg
     1441 ccggctcgca tcgtcgcgct cggcgagcgt gaggatcgcg ttcgaaaacc cgcgatgctc
     1501 gggcttcgag agcaggccat ggccgacaat gacgtcgttg ggaattggct gcgtccactc
     1561 gagcgtgcgg acgtgacgct cgggcgcgac atgcagaaag cctgcgcgca cgaccggctg
     1621 tccttcgtcg gggcgctggc catacgtcgc accgacgtcc gcgcggccgt cgagcaccgc
     1681 gcgcacgacc tcggcgtggg agcgcaggaa cagctcttgg cgaaagtggt gagccgcgtc
     1741 gaatccacgc gccgcgagat gcacgcgcgg cacgatgtag cccgccgccg acgagcgatc
     1801 gacccacgcg acgacaccgt tctgcacatc ctcgagcgag cgaaacggtt tcttgccgtc
     1861 gacgaaccac accgaccggt aatcggtgcg gtcgtcgcgt accgccgtca gcagcggccg
     1921 cagctggatg ttctcgttcg tcaggatcat cagcgtcggc gacatccacg cgtagtcgac
     1981 gcgatccttc tcgagctcga gcgcgagctc cgagtacgat gcgcagttaa tgccgtgtac
     2041 cggcgtgtcg agtagcttcg tgagcgcgat acacagcgag tcgagggcga gccgcgcgtc
     2101 accgggcgtc gtcgagggga ccacagcaag cgtgatctcg cccatttctc ccagtgtacc
     2161 aggtaacgtg cagcggcttg caatcgacag cgcgcaccgc gggactgacg gcagcggcga
     2221 tgatcggctt cgccgcgaac tcgctgcttt gtcgcgccgc attgcggggt ggcgcgatcg
     2281 atgccgccag cttcactgcg gtgcgcctcg ttaccggagc tgccgtcctt gtattgatcg
     2341 tcgcagcccg caaagacagc gtccgcggtg aaggtacgtg gctgtcggcg tttgcgctcg
     2401 cgggctatgc ggtcgcattc tcgttcgcgt acctcgagat cggcgccgcg acgggtgcgc
     2461 tgctgctgtt cggcgcggta cagctgacga tgattgcggg cggcctgatt cgcggcgaac
     2521 gcccgacgcc gcggcaatgg cttggctggc tcgtcgcgct ttccggc
//
LOCUS       DASUFX010000828         5479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_133896,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000828 DASUFX010000000
VERSION     DASUFX010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133896"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFV99_33360"
     CDS             <1..842
                     /locus_tag="VFV99_33360"
                     /inference="COORDINATES: protein motif:HMM:NF020586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064327.1"
                     /translation="ENHANAVADRVVQGKPAGDLLAPFAGGQGGGKAVQSKAVQFLGH
                     KLGTPLPEGAEEPQYGEDPDQRRYSREQYERMWEEEQGHKLKDGEKSTIKRGCIGITA
                     NNIAGGGNPLQYAVGTFGDFEHAHQFMDGKNKELHEMRADPKQAHLAPAGEYVLFAKL
                     FWSNQKDGDNSKPDDKAFKADPKTGRMDMTGYKYKAQPGYVNFDYGFWDDSQQCFWHA
                     NHMEYGDPDKAKKDPMKVYQSTKEHFVRGYIDFDRIVFGVALATNYDPGKAAIDTASR
                     GGH"
     gene            845..1120
                     /locus_tag="VFV99_33365"
     CDS             845..1120
                     /locus_tag="VFV99_33365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064328.1"
                     /translation="MSLEDKVGTTVTIQGVARNAMAGAVVLTDDRTPVYVAGLDRWDR
                     ATDGKPVKATGTLRKQGGDNVVDAHGNYSSGAPGDRFVLEQPTWTVG"
     gene            1117..1512
                     /locus_tag="VFV99_33370"
     CDS             1117..1512
                     /locus_tag="VFV99_33370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064329.1"
                     /translation="MTRLAASFALLAACSSSKPSEPTTPNGSGSATPTPNAAPAVITD
                     DTGAKANAGAVVDVKGTARDAKLGAVVVASSLVVYCLGVEHWPDDVFGKPVTAHGKLE
                     QTTEFAAQDETSAGTTGPVWVLRDCKYGP"
     gene            complement(1520..2989)
                     /locus_tag="VFV99_33375"
     CDS             complement(1520..2989)
                     /locus_tag="VFV99_33375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064330.1"
                     /translation="MRLRAWLVVVLAACGDNHGSTHDASVDSAVDASIDASEECVTSC
                     DDGDACTADACVHGTCHNTPRTTCGEAPICGSAACAVTDDDEDGIDDVSEARGYVDSN
                     CNGVDDGPDVDLRLPGAVVGRKTIYLEYDYMAKPGSGTACTTDADCSAVEACECPMAA
                     PCAARECQHSHRPRAAALDAIVAAFAAKNIDLVIEPSPTKLVETDVITYGSLAPTCAG
                     PSASNFFAIKSASFDPRKRWISHYAIFGHYATCNSGVNCATCPADPITGVPPSFGVSG
                     TAEIRGNDFIVTMARAYIDFNLPRTLANEGGTIMHELGHNLGLLHGGHEPSPEGKANY
                     FSVMNRAFQFTGITSAANGMRLDYSGAVLATIDENALVESTGLDEASADPLHSDDFAH
                     ITNGQGIDVDAPGTGPVDWDGDGSIASATLTSPVDVNSDGSYTMMVGSNDWARLVLPF
                     ACEPSGMANGHVPQATVIESPALASWLPSPGSVGTSRGR"
     gene            3054..4382
                     /locus_tag="VFV99_33380"
     CDS             3054..4382
                     /locus_tag="VFV99_33380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064331.1"
                     /translation="MKLVLIFVACVACGGPTAAPSRTVDRRAPASSDGGAMVAAPDDT
                     GAAVSDVPWPRSASRTASSFVSERWTFAESKLKPLFEPIETVSRPENMFVTADARNMI
                     IVGNLRDYEVRSPHKSTHDPTRELAWPYVRLSPRGDYAAVVTESRPSKWDVGSDVAVI
                     RFSDHAVMTTIEGGADPFWIDSSVLAFRVGNQAQRLELPSLKVTDVGSPQTTHGCPNA
                     ALRGYTRVDESMCPSGRYSRVVAVDAAYTKWLVVDDDGAHETALRLVDLASGRDRVLA
                     DRKSHGYPLYVTVSPHGTRYCMTPKHGKAGYRLYCGRFPEADDQVKVMELSGDTLPRT
                     DWLDDDRFLLSPNKIIDLANGKQLELDAKLASATTLSGNLGPHYVVARMGWVYVLDLE
                     TKTWTRLDTKPAPDATDVLDQATAAGRLWVTQITARAATTDLFELRLVPK"
     gene            complement(4387..4920)
                     /locus_tag="VFV99_33385"
     CDS             complement(4387..4920)
                     /locus_tag="VFV99_33385"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064332.1"
                     /translation="MTTTAKPLKLSEADLRLIARWVSACAARALPLFEVAAPRDTRPR
                     DALAGIRLFVRGGKRTAQLRSLALAALAAAREVGDPVATAAARAAGYAAASAYTHPLA
                     SPHQIKHILGPVVYAARARELAADDATAGDKELASALRRATPALRNVVRRFPSRRAGR
                     SRLDQLYSQLDAGLRRR"
     gene            complement(4931..5410)
                     /locus_tag="VFV99_33390"
     CDS             complement(4931..5410)
                     /locus_tag="VFV99_33390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064333.1"
                     /translation="MHSVLHTLDSFGGIYVAMFLFAILSGVFPLANSEAAMVALGAAS
                     SYSVPKLLVLAVITALGQSVMHASLYLSARGIANAGAKNRPKLEARIAKARELGARWE
                     KSELLLIALGATVGIPPQVLVALFAGVIGIRLRTFVAIDVAGRIARFVTIVMVAHLV"
BASE COUNT         1056 a         1777 c         1751 g          895 t
ORIGIN      
        1 acgagaatca cgcgaacgcg gtcgccgatc gtgtcgtcca aggcaaaccg gccggcgatc
       61 tactcgcgcc attcgcgggt ggacagggtg gcggcaaggc ggtgcagtcg aaggccgtgc
      121 agttcctcgg tcacaagctc ggcaccccgc tgcccgaggg cgccgaggaa ccccagtatg
      181 gcgaggaccc cgatcagcgg cggtactcgc gcgagcagta cgagcgcatg tgggaagagg
      241 agcagggcca caagctgaag gacggcgaga agagcacgat caagcgcggc tgcattggca
      301 tcaccgcgaa caacatcgca ggcggcggca acccgctgca gtacgcggtc ggcacgttcg
      361 gcgacttcga gcacgcacat cagttcatgg acggcaagaa caaggagctg cacgaaatgc
      421 gcgccgatcc gaagcaagcg cacctcgcgc ctgcgggcga gtacgtgctg ttcgcgaagc
      481 tgttctggtc gaatcagaag gacggcgaca attcaaagcc cgacgacaag gcgttcaagg
      541 ccgatccgaa gacaggtcgc atggacatga ccggctacaa gtacaaggcg cagcctggct
      601 acgtgaactt cgactacggg ttctgggacg acagccagca gtgcttctgg cacgcaaacc
      661 acatggagta cggcgatcca gacaaggcca agaaggaccc gatgaaggtc taccagagca
      721 cgaaagagca cttcgttcgc ggctatatcg atttcgatcg catcgtgttt ggcgtcgcgc
      781 tcgcaactaa ctacgatccc ggcaaggcgg cgatcgacac tgcgagccgc ggaggacact
      841 agccatgagt cttgaagaca aggtcggcac gacggtcacg atccaaggtg tcgcgcgcaa
      901 cgcgatggcc ggtgccgtgg tcctcaccga tgatcgcacg ccggtctatg tcgctggcct
      961 cgaccgctgg gatcgcgcga ccgacggcaa gccagtcaaa gcgaccggaa cactgcgcaa
     1021 gcaaggtggc gacaacgtcg tcgacgcgca cggcaactac agcagcggcg cgcccggcga
     1081 ccgcttcgtg ctcgaacagc cgacctggac cgtcggatga cgcggctagc tgcatcgttc
     1141 gcgctgcttg ccgcgtgcag cagcagcaag ccatcggagc cgacgacgcc caacggttcc
     1201 ggttccgcga cgccgacccc gaacgccgcg cccgccgtca tcaccgatga cacgggtgcg
     1261 aaggcgaacg ccggcgccgt cgtcgatgtc aaaggcacgg cgcgcgatgc caagcttggc
     1321 gcagtcgttg tcgcgtcgag cctcgtcgtc tattgcctcg gcgtcgagca ttggcccgat
     1381 gacgtattcg gcaagccggt caccgcacac ggcaagctgg agcagaccac cgagttcgcg
     1441 gcgcaagacg agacgagcgc cggcaccacc ggccccgtgt gggtgctgcg cgactgcaag
     1501 tacggcccgt agtcggcgat catcgaccac gtgatgtgcc aaccgagcca gggctcggca
     1561 gccacgaggc gagtgccggc gactcgatga ctgtagcttg cgggacgtgc ccgttcgcca
     1621 tgcctgatgg ctcgcacgcg aatggaagca cgagtctggc ccagtcgttt gatccgacca
     1681 tcatcgtgta gctgccatcc gaattaacgt ccacgggcga cgtcagggtt gccgaggcga
     1741 tcgagccgtc gccatcccag tcgacgggcc cggtacccgg agcgtcgacg tcgatgccct
     1801 gaccgttggt gatgtgcgcg aaatcgtcgc tgtgcagcgg gtcggcgctc gcttcgtcga
     1861 ggccggtgct ttcgacaagt gcgttctcgt cgatcgtcgc gaggacggcg ccggaataat
     1921 cgagacgcat accgttagct gcgctcgtga tgccggtgaa ctggaatgcg cgattcatca
     1981 cggagaagta gttcgccttg ccttccggcg atggctcatg accgccgtgc agcaaaccga
     2041 ggttgtgacc gagctcgtgc atgatcgttc cgccttcgtt cgcgagcgtt ctcggcagat
     2101 tgaagtcgat gtaggcacgc gccatcgtta cgatgaaatc gttgccgcga atctccgccg
     2161 tcccactcac tccgaacgag ggtggtacgc cagtgatcgg atctgccgga cacgtcgcgc
     2221 aattgacacc cgagttgcac gtcgcgtaat ggccgaagat cgcgtagtgc gagatccatc
     2281 gctttcgcgg gtcaaagctc gcactcttga tcgcaaagaa gttcgacgcg ctcggtccgg
     2341 cgcaggttgg agctagcgag ccgtaggtga tgacgtctgt ctcgaccagc ttcgtcggcg
     2401 atggctcgat tacgagatcg atgttcttcg ctgcgaacgc cgcgacgatt gcatcgagcg
     2461 cagcagcgcg cggccgatga ctgtgctggc attcgcgggc cgcacacggc gccgccatcg
     2521 ggcattcgca cgcttctacc gccgaacaat ccgcatctgt cgtacacgcc gttccgctac
     2581 ccggcttcgc catgtagtcg tattcgaggt agatcgtctt gcggccgaca acggctcccg
     2641 gcaggcgcag atcaacatcc ggcccgtcgt cgacaccgtt acaattcgag tctacgtagc
     2701 cgcgcgcctc cgaaacgtcg tcgatgccgt cttcatcgtc atcggtgacc gcgcacgcgg
     2761 cgctaccgca tatcggcgcc tcgccacacg tagtacgcgg cgtgttgtga caggtcccat
     2821 ggacgcaagc gtccgccgta caggcatctc cgtcgtcgca cgaagtcacg cattcctcgg
     2881 acgcgtcgat gctcgcatca accgcggagt cgacgctcgc gtcatgcgtg ctgccgtgat
     2941 tgtcaccaca cgccgccaac accacgacga gccacgccct gagtcgcatc catgcagtgt
     3001 cgctcaagat ggctcgcacg agcaccgtta gctgtgacat aagctgacgc gtggtgaagc
     3061 tcgtgctgat attcgtcgct tgtgtcgcgt gtggcggccc caccgcagcg ccctcgagaa
     3121 ccgtcgatcg ccgcgcgccc gcgtcgagcg atggcggtgc gatggtggcc gcgccggacg
     3181 acaccggtgc agcggtctcc gatgtgccat ggccgcgatc tgcctcgcgc actgcatcgt
     3241 cgttcgtgag cgagcggtgg acattcgccg agtccaagct aaagccgctg ttcgaaccga
     3301 tcgagacggt ctcgcggcca gaaaacatgt ttgtcaccgc cgacgcgcgg aacatgatca
     3361 tcgtcggcaa cctgcgcgat tacgaagtgc ggtcgccaca caagtcgacg catgacccga
     3421 cgcgcgagct cgcgtggccg tacgtcaggc tgtcgccgcg tggcgactat gcggccgtcg
     3481 tcacggaatc gcggccgtcg aagtgggacg tcggcagcga cgtcgccgtg attcgcttct
     3541 ccgaccacgc agtgatgacg acgatcgaag gtggtgccga tccgttctgg atcgatagct
     3601 cggtcctggc gttccgcgtc ggtaatcagg cacagcgcct cgagcttccg tcgctcaagg
     3661 tgaccgacgt cggatcgccg cagaccacgc atggttgtcc gaacgcagcg ctgcgcgggt
     3721 acacgcgagt cgacgaatcg atgtgcccga gtggtcgcta cagccgcgtt gtcgccgtcg
     3781 atgcggccta taccaagtgg ctcgttgtcg acgatgacgg cgcgcatgag acggcactgc
     3841 gcctggtcga cctcgcgtct ggacgcgatc gcgtgctcgc ggatcgcaag tcgcatgggt
     3901 atccgctcta cgtgacggtg tcgccgcacg gcacgcgcta ctgcatgacg ccgaagcacg
     3961 gcaaagccgg ttatcggctc tattgcggtc gattccccga ggccgacgat caagtcaaag
     4021 tcatggagct ttccggcgac acgctgccgc gcacggactg gctcgatgac gatcgatttc
     4081 tgctgtcgcc gaacaagatc atcgacctgg cgaatggcaa acagctcgag ctcgacgcca
     4141 agctcgccag cgcgacgaca ctaagtggca acctcggacc gcactatgtc gtcgcgagaa
     4201 tgggctgggt gtacgtactc gatctcgaga cgaagacatg gacgcgcctc gacaccaagc
     4261 cggcaccgga tgcgacggac gtgctcgatc aggcaacggc cgcagggcgg ctctgggtga
     4321 ctcaaataac cgcaagagcg gcaacgacgg atctgttcga gctgcgcctc gtgcctaaat
     4381 gaggtgctac cgccggcgca gacccgcgtc gagctgagag tagagctggt cgagtcggct
     4441 acgcccggcg cgacgcgaag gaaagcggcg cacgacgttg cgcaaagcgg gcgtggcacg
     4501 ccgcagtgcc gacgcgagct ccttgtcgcc ggcagtcgcg tcgtcagctg cgagctcgcg
     4561 agcgcgggcc gcatacacca ccggcccgag gatgtgtttg atctgatgcg gcgacgcgag
     4621 cggatgggtg tacgcgctcg ctgctgcgta cccggctgca cgagccgccg cagtggcgac
     4681 cgggtcaccg acttcgcgcg ccgccgcaag tgcggccaac gcgagcgagc gcagttgcgc
     4741 cgttcgcttg ccaccacgca cgaacagccg gatccctgcc agcgcgtcac gcggacgcgt
     4801 atcacgcgga gccgcgacct cgaacagtgg cagcgcacgc gccgcgcacg cgcttaccca
     4861 gcgtgcgatg agacgaagat ccgcttcgct cagtttcaac ggcttcgcag tagtggtcac
     4921 tcgcacggct tcacacgagg tgcgcgacca tgacgatcgt gacaaagcgt gcgatgcgtc
     4981 cggcaacatc gatcgcgaca aatgttcgga gccggatacc gatcacgcct gcgaacaacg
     5041 cgacgagcac ctgcggcgga atcccgaccg tcgcgcccag cgcgatcagg agcagctcgc
     5101 tcttctccca tcgcgcaccg agctcgcgtg ccttcgcgat ccgtgcctcg agcttcgggc
     5161 ggttcttggc tcccgcgttc gcgatcccgc gcgccgacag atacagcgac gcgtgcatca
     5221 ccgactgacc gagcgcagtt atcacggcaa ggacgagcag cttcggcacg ctgtacgagg
     5281 acgcagcgcc gagggccacc atcgcagcct cgctgttcgc gagcgggaac acacccgaca
     5341 ggatcgcgaa cagaaacatc gcgacgtaga tcccaccgaa gctatccagc gtgtggagga
     5401 cggaatgcac cccacacatg acgtagcacg tgatgccccg ctacgtaggg atacgcgtga
     5461 ccaacgcatc gcacagtgc
//
LOCUS       DASUFX010000829         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1246972, whole genome shotgun sequence.
ACCESSION   DASUFX010000829 DASUFX010000000
VERSION     DASUFX010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246972"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFV99_33395"
     CDS             <1..305
                     /locus_tag="VFV99_33395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064334.1"
                     /translation="HRKALITRVVEGDGRSNSQQRRAAFAGDFGPTGAAALTEKVVKH
                     AYKVTDEDVAAAKAAGLTEDQIFELVVCSAIGEANRQLETALAALDEATKEVSNAS"
     gene            295..540
                     /locus_tag="VFV99_33400"
     CDS             295..540
                     /locus_tag="VFV99_33400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064335.1"
                     /translation="MRLEILNGGHSFKFKAMFAMMKLFTRQRVPDVVKTLTYRPDFFG
                     NPLNECFQEAMRGQSAWSVGDRELMAAYVSKMNECEF"
     gene            592..879
                     /locus_tag="VFV99_33405"
     CDS             592..879
                     /locus_tag="VFV99_33405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064336.1"
                     /translation="MSAALENVDSAPLEEPLRATLRLLRKLTKERTVTADDMRAVLAA
                     GATKEQIEDALAVAFCFNVIDRCADTFEFFVPGPEGFAFSAKFLLSKGYKL"
     gene            1032..2036
                     /locus_tag="VFV99_33410"
     CDS             1032..2036
                     /locus_tag="VFV99_33410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064337.1"
                     /translation="MTHALVLSFSMLVVACTADVEIDQLPPEPELSPAEVAELPDPAT
                     DVEHDVSLLVEGPAEDDAATFDDAAAELIYDEPVVDDDETQSATTTIAFQPLLKYGLH
                     PRASDGLRTAGVVAWRITQTIGNAPASAGYHAQDGTVNGHPYTAAVDLSVSGMSTTQI
                     HNLLERLAKVGFAAWYRRNGADGWVGANHIHAVYANCKMKSQLRSQVRSWLVGRNGLV
                     SNTIYKFHTFTTTAKSIVKAKFAQSNSGTTNGGAGASGRVNTAGAPLTIRSAASTSAS
                     AVGSVADGAYVTITCQKTGTSVKGTYGTSTLWDKISGGYVADAYVSTGSDGRVAPLCP
                     "
     gene            2056..2940
                     /locus_tag="VFV99_33415"
     CDS             2056..2940
                     /locus_tag="VFV99_33415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5064338.1"
                     /translation="MRRAAIPDLPFRHNTRPGLGFEIFRMSELFARVLPHELEAPQRP
                     EFHIVYLGLRGAGSLVVDFSKVPLGANTLTVVARGRVQHFLPESGVDAWMLLAAPEFI
                     ALDARSVDPLRGASVLDPGWATPALKLPAGDRIELLALADQLAAEQARPLDGFQPALM
                     SSLLRAFLLRAERVALASDARPTLPPALARFFTTLEQDCTKTRSVAHYAKAAGVSPRR
                     LAELLVDHTGKSTKLVIDERVVLEQKRLLAHTDVSVKELADRTGFDEPTNLVKFFRHH
                     TGTTPDAFRRNLPFGRRS"
     gene            2979..3854
                     /locus_tag="VFV99_33420"
     CDS             2979..3854
                     /locus_tag="VFV99_33420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5064339.1"
                     /translation="MSKRKTLLASLVAIAASSPAFAEPKLTTTVYTASPGGFLVNSTL
                     VAGEKDAVLIDGQFTLADAHRLVAMVLESKKNLTTVYVTHYHPDHYFGLVVIKQAFPK
                     AKLVATPETIKEIRKTWATKVKQWGAMYGDLVPKNPLVPAPLTGNALTLEGQTLEVHS
                     HVMGDSADSSYVWIPSIKTVVTGDIVFNGVHPWTAESSTETRKSWIKSLDEISALKPT
                     TVVAGHKDPKLADDASALRATRDYVEAFDAAVASSKTAAEVKDKVSKKYGSLQLPIIL
                     EIGAGAALPSATPSK"
     gene            3985..4416
                     /locus_tag="VFV99_33425"
     CDS             3985..4416
                     /locus_tag="VFV99_33425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064340.1"
                     /translation="MQLFPLEVVESWHSAVKAHNVEQLCALSSKDVEVVGVRETGRGH
                     DELRRWFRRGGLSAEPIRWFCDSRGHVVVEQRGRWFTPEAVTVEDSGRWFIPDTTHER
                     IVATAFSVVAGRVVRYQRFEKLADALSATKLTDDDEVLPTV"
BASE COUNT          805 a         1503 c         1491 g          732 t
ORIGIN      
        1 ctcaccgtaa ggctttgatc acgcgtgtcg tcgagggcga cggccggtcg aacagccagc
       61 aacgtcgcgc agcctttgcc ggcgactttg ggcccaccgg cgcggccgcc ctgaccgaaa
      121 aggtcgtcaa gcacgcttac aaggtgaccg acgaagacgt cgcggcggcc aaggcggctg
      181 ggctcaccga agaccagatc ttcgagctcg tcgtgtgctc ggcgatcggc gaagcgaatc
      241 gccagctcga gaccgcgctc gcggcgctcg acgaagcaac gaaggaggtg agcaatgcgt
      301 cttgagattc tcaatggcgg tcactccttc aagttcaaag cgatgttcgc gatgatgaag
      361 ttgttcacgc ggcaacgcgt gcccgacgtc gtcaagacgc tgacgtatcg ccccgacttc
      421 ttcggcaacc cgctcaacga atgctttcaa gaagcgatgc gcgggcagtc ggcgtggtcg
      481 gtcggcgatc gcgagctgat ggccgcgtac gtctcgaaga tgaacgagtg cgagttttga
      541 acgaaagctc acgccgcggt cgcggcgaaa gcgtacgaga acgaagggaa ggtgagcgcg
      601 gcgctcgaga acgtggatag cgcgccgctc gaggagccgt tacgcgcgac gctgaggctg
      661 ctgcgcaagc tcacgaagga gcgcacggtg accgccgatg acatgcgcgc ggtgctcgcg
      721 gctggcgcaa cgaaagagca gatcgaggat gcgctcgcgg tcgcgttctg cttcaacgtg
      781 atcgatcgct gcgccgacac gttcgagttc ttcgtccccg gcccagaggg attcgcgttc
      841 agcgcgaagt tcctgttgtc gaagggctac aagctctagg gcgaagagtc gtgagcacgt
      901 gtagcggcgc ccgctacacg cgcgttgcgt cgtgtcgcga tttcgcgcag ctgcgcgtgg
      961 accggcaatt gcgttagcca ccgccatcgc gagcgccggc tcgctgagga ggaatcgcta
     1021 tgtcgcgaag gatgacgcac gccctggttc tgtcgttcag catgctcgtc gtggcgtgca
     1081 ccgcagatgt cgagatcgat cagttgccgc ccgagccgga attgtcgccg gctgaagtcg
     1141 ccgagctgcc cgatccggcc accgatgtcg agcacgacgt gtcgctgctc gtcgaaggac
     1201 ctgccgagga cgacgccgcc acgttcgatg acgctgctgc cgagctgatc tacgacgagc
     1261 cggtcgtcga tgacgacgag acgcagagcg cgaccaccac gatcgcattc cagccgctgc
     1321 tcaagtacgg cctgcatccg cgcgcatcgg atggcctgcg caccgccggt gttgtcgcgt
     1381 ggcgcatcac gcagacgatc ggcaacgcac cggcgtcggc gggctatcac gcgcaagacg
     1441 gcaccgttaa cgggcacccg tacactgcgg cggtcgactt gtcagtgagc ggcatgtcga
     1501 cgacgcagat ccacaacctc ctcgagcggc tcgcgaaggt cggcttcgcg gcgtggtacc
     1561 gccgcaacgg tgccgatggc tgggtcggcg cgaaccacat ccacgcggtg tacgcgaact
     1621 gcaagatgaa gtcgcagctg cgctcgcagg ttcgctcgtg gctggtcggc cgcaacgggc
     1681 tcgtgagcaa cacgatctac aagttccaca cgttcacgac gaccgcgaag tcgatcgtca
     1741 aagcgaagtt cgcgcagagc aactccggca ccacgaacgg cggcgctggc gcgtcggggc
     1801 gcgtgaacac ggcgggcgcg ccgctgacga tccgctccgc cgcgagcacg agcgcgagcg
     1861 cggtgggctc ggtcgccgac ggcgcgtacg tcacgatcac gtgccagaag accggcacgt
     1921 cggtgaaggg cacgtacgga acgtcgacgc tatgggacaa gatcagcggc ggctacgtcg
     1981 cggatgccta cgtctcgacc ggctccgacg gacgcgtcgc gccgctctgc ccgtagctgc
     2041 gctaccgtgc ggccgatgcg ccgcgcggcc atccccgacc tgccgtttcg gcacaacacg
     2101 cggcccgggc tcggcttcga gatcttccgg atgtcggagc tgttcgcccg cgtgcttccg
     2161 cacgagctcg aagcgccgca gcggcccgag tttcacatcg tgtatctcgg gctgcgcggt
     2221 gccggttcgc tggttgtcga ttttagcaag gtgccgctcg gcgcgaacac gctgactgtc
     2281 gtcgcgcgcg gtcgcgtgca gcacttcctt cccgagtcgg gcgtcgacgc ctggatgctg
     2341 ctcgcggcgc ccgagttcat cgcgctcgat gcgcgttcgg tcgatccgct gcgcggtgca
     2401 agtgtgctcg acccgggctg ggcgacgccc gcgctgaagc tgccggccgg cgaccgcatc
     2461 gagctcctcg cgctcgccga ccagctcgcg gccgagcagg cccgaccact ggatgggttt
     2521 cagcccgcgc tgatgtcgtc gctcttgcgg gcattcttgc tgcgcgccga gcgggtcgcg
     2581 ctcgccagcg atgcgcggcc gacgctgcca cccgcgctgg cgcggttttt caccaccctg
     2641 gagcaggact gcacaaagac ccgctcggtc gcgcactacg cgaaggcagc gggcgtatcg
     2701 ccgcgccggc tcgccgagct gctcgtcgac cacaccggca agtcgacgaa gctggttatc
     2761 gacgagcgcg tcgtgctcga gcagaagcgg ctgctcgccc ataccgatgt ctcggtcaag
     2821 gagctggccg accggacggg cttcgacgag ccgaccaatc tcgtgaagtt cttccgccac
     2881 cacaccggga cgacacccga tgcgttccgc cgaaatttac cattcgggcg ccgatcctga
     2941 gtctgccgcc ttcggccggc cgcgactagg ttgccggcat gtcgaagcgc aaaaccctcc
     3001 tcgcctccct cgtcgcgatc gccgccagct cgcctgcgtt cgcggaaccc aagctcacca
     3061 cgaccgtcta caccgcctcg ccgggcggct tcctcgtcaa ctcgacgctg gtcgccggcg
     3121 agaaggacgc cgtcctcatc gacggccagt tcacgctcgc cgacgctcac cggttggtcg
     3181 cgatggttct cgagagcaag aagaacctga ccacggtcta cgtgacgcat taccaccccg
     3241 accattactt cgggctggtc gtgatcaagc aggcattccc gaaggccaag ctcgtcgcaa
     3301 cccccgaaac gatcaaggag atcaggaaga cgtgggccac gaaggtgaag cagtggggcg
     3361 ccatgtacgg cgacctcgtg ccgaagaacc cgctcgtgcc cgcgccgttg accggcaacg
     3421 cgctcacgct cgaaggccag acgctcgaag tccattcgca cgtcatgggc gactccgcgg
     3481 acagctcgta cgtctggatc ccgtcgatca agaccgtcgt caccggcgac atcgtcttca
     3541 acggtgtcca tccgtggacc gcggagtcgt caaccgagac acgaaagtcg tggatcaagt
     3601 cactcgacga aatcagtgca ctgaagccca cgaccgtcgt cgccggtcac aaggatccga
     3661 agctcgccga cgacgccagc gcgctcaggg cgacgcgcga ctacgtagag gcgttcgatg
     3721 cggccgtcgc gtcgtcgaag accgcggccg aggtcaaaga caaggtgagc aagaagtacg
     3781 gctcgctgca gctgccgatc atcctcgaga tcggggctgg cgccgcgttg ccgtcggcaa
     3841 caccgagcaa gtgacgttga cgtggcgtca aggaggcggc tcgtgactga taacagcaga
     3901 tcccaaccgc cgaattgtca gcaacgtgct gcaagcaagt tgcgcgttga tcgtcgtcgg
     3961 aagctcgact agtttgcccc gcatatgcag cttttccctc tcgaggtcgt cgagagctgg
     4021 cactccgcag tcaaggcgca caacgtcgag cagctgtgcg cgctgagctc gaaggacgtc
     4081 gaggtggtgg gcgtgcggga gacgggtcga ggtcatgacg agctgcggcg ctggttccgg
     4141 cgcggtggtc tcagcgcgga gccgattcgc tggttctgcg actcgcgcgg tcacgtggtc
     4201 gtcgaacaac gcggtcgctg gttcacgccc gaagcagtca cggtcgagga cagcggccgc
     4261 tggttcattc ccgacacgac gcacgagcgc atcgtcgcga cggcgttctc ggtcgtcgcc
     4321 ggccgcgtcg tgcgctacca gcgcttcgag aagctcgccg acgcactcag cgcgacgaag
     4381 ctcaccgacg acgacgaagt actaccgacg gtctagagct cctcgatcac gagccgaccg
     4441 ccgcgctggc gaatcgtgaa gcggcggacc gctggctcgt agccgcgccc cgcgaagttg
     4501 cgcggcaaca cctcgacgtc gccgacgcga c
//
LOCUS       DASUFX010000830         7013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2081860, whole genome shotgun sequence.
ACCESSION   DASUFX010000830 DASUFX010000000
VERSION     DASUFX010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081860"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFV99_33430"
     CDS             <1..444
                     /locus_tag="VFV99_33430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5064341.1"
                     /translation="KSDPSGAHCLHNTNCCDAITATNQGCYRAQCYATARELASRLGL
                     TDYTVCFQSRLGRTPWIQPHTDVLLDELAKQGKKRLAVLCPAFVADCLETLEEIGIRA
                     REQFKAAGGEELTLVPSLNATPAWVDAVCAMAERHAARKQLPVVT"
     gene            complement(449..1552)
                     /locus_tag="VFV99_33435"
     CDS             complement(449..1552)
                     /locus_tag="VFV99_33435"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5064342.1"
                     /translation="MSDPRAQLQAMLRPSAGGLYVVSTGRAAQLEVQRHLYGVSDEAS
                     IQNRFIAALDRIANAKAIILGVPSDVGAGYRRGANLGPQAIRSALIAADPDLRGWLDR
                     RSIVDIGDVAVVPQLLHDAMLAPAQIEASRAALYKDVAPGLAAELPVSPLSIEARVLD
                     CVLAINPRVIPIVLGGDHSVAWPVSETLAKHRRDRWGIVQPDAHTDLLAERLGIKYCY
                     ATWSYHANELLGRDGRLVQVGVRASRFGREHWESTLGVRQFWAAEILRDPDAALDAVI
                     DHLRAKGVTSVYFSNDIDGTDAKHAEATGTPEPDGLEPDWLFALIHRIGKDIGLCAAD
                     CVEVAPPLGPTAEATDATTRLAARYVRACLELI"
     gene            1676..3145
                     /locus_tag="VFV99_33440"
     CDS             1676..3145
                     /locus_tag="VFV99_33440"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5064343.1"
                     /translation="MNRLFRPTIVAALGIAASASIASANVEIGGTAGIHVFSTDNELG
                     VPDTDDAPSERNSALFGLRLGVTFNDMLGVEGEFGVIPSESRELVFDVWNLTYRAHLI
                     AQFRAANPATKLVPFVVFGGGAMAVVDSKNEVKIDKDTDAMLYAGVGAKYRVDNGWGL
                     RADARILFPPSSAGNGPTVDFEALLSIYKEFGRKAAEKVVEQPKGPNDTDGDGLTDDV
                     DKCPNEAEDKDGFQDDDGCPDNDNDGDGVPDASDKCAMEPEDKDGFEDDDGCPEADND
                     KDGIPDASDKCPNEAEDKDGFQDDDGCPDPDNDGDGVPDGSDKCPDQPETKNGYQDDD
                     GCPDEIPQKVKQFTGVIQGINFKVNDAALLPASNKTLDKAVAVLKEFGDLKLEIQGHT
                     DDQPIKAGGKFADNKALSQARAETVKAYFVSKGIEEGRLTAVGFGDEQPVVAPTGLKG
                     AKLNSARAKNRRVEFKLVSTLVPGGGAAQGGGGAMPPPP"
     gene            3305..3532
                     /locus_tag="VFV99_33445"
     CDS             3305..3532
                     /locus_tag="VFV99_33445"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX5064344.1"
                     /translation="MALSRELLDWLVCPKSKQPLVYFEKEGFLLCPESRLRYRIENDV
                     PVLLVEEATEVSGPEIERLLKQAKELGLPGA"
     gene            3601..4548
                     /locus_tag="VFV99_33450"
     CDS             3601..4548
                     /locus_tag="VFV99_33450"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5064345.1"
                     /translation="MLKSLSIFTGNSNRALADEICKFVEIPLGRADVTHFSDGEIYVE
                     IGENVRGVNCFVVQPTCSPPNQALMELLIMIDALKRASAGSIVAIVPYFGYARQDRKA
                     KPRTPITAKLVANLITAAGADRALAMDLHAGQIQGFFDIPFDHLFAMPVLIEELRVLG
                     FGGDQTVVVSPDAGGVERARAFSKRLGASLAIIDKRRPAPNVSEVMNIVGDVKGKKAV
                     IVDDIIDTAGTLTHAAAAIVNAGAASVSACASHAVFSGKAVPRITDSALQHVIVTNTI
                     PLNEAGQHCEKVRVKSVGRLLGEAIKRIHHGDSISSLFV"
     gene            4572..5351
                     /locus_tag="VFV99_33455"
     CDS             4572..5351
                     /locus_tag="VFV99_33455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5064346.1"
                     /translation="MPEAQVGKLTVTLRNNSGKGAAIKLRQQGKVPGVCYGASPEGRI
                     DPMPIVVDVKALRAALDPVRKQNTVIDLTIDGGSAPRKLHALVKEYQLHPLRREITHV
                     DLLAIDPNKEVSAQVPLEFVGKPAGAIDGGQIRIVIRALEVKAKPSDIPVKLTIDVSP
                     LEIGDVIHVNAIALPSGVTALTGRDLAVVTCAKPEEEKVEAAAVTPEAAAAAAAGGAA
                     PAAGAAAAGAAPAAGAKDAKAAAPAAGAKDAKAAAAPAKKK"
     gene            5500..6111
                     /gene="pth"
                     /locus_tag="VFV99_33460"
     CDS             5500..6111
                     /gene="pth"
                     /locus_tag="VFV99_33460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5064347.1"
                     /translation="MTWLVVGLGNPGSKYERNRHNIGFRVVDELVRKHGLPAWKSAIK
                     GGESTSGMITTERGREKAFVLKPLEFMNLSGFATQRAAAFHDVDITNILVVHDEIDLD
                     FGIVRLKNGGGHGGHNGLRSIQEQLGGGGYARLRMGVGRDPNKPPGSKDAANWVLADF
                     PSAHQATVDAMISAGCECIETTLALGIVAAMNKHNARPNLATS"
     gene            6176..6709
                     /gene="rpsF"
                     /locus_tag="VFV99_33465"
     CDS             6176..6709
                     /gene="rpsF"
                     /locus_tag="VFV99_33465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5064348.1"
                     /translation="MARLQSLRDTPNTAREYETIYILRPNTPNEGVAEVNTRIKNVIE
                     SMGGKVIKVDNWGKRRLAYEVAKERKGIYLYWQYLAQPGVVEECERNLRMLDNVIRYL
                     TTKVDENIDVTARPTEIDDSSYEKAAQTAADEEDLFLSRGSEELPSSDEDDEFGQDDF
                     NEEPSSGGMSGGLGEKE"
     gene            6725..>7013
                     /locus_tag="VFV99_33470"
     CDS             6725..>7013
                     /locus_tag="VFV99_33470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064349.1"
                     /translation="MDAGDEGLDRDGDDRRGGKGRGAARRKLPWPMDQIVMIPTLRLQ
                     STSVGTELHADEAWKGVDFQFWQVRGNTASDNDMEDNFVTGERGAPQIIETF"
BASE COUNT         1363 a         2253 c         2299 g         1098 t
ORIGIN      
        1 aaaagcgatc cgagcggcgc ccactgcctc cacaacacga actgctgtga cgcgatcacc
       61 gcgacgaacc agggctgcta tcgcgcgcag tgttatgcga ccgcgcgcga gctcgcgtcg
      121 cgactcggcc tcaccgatta caccgtctgc ttccagtcgc ggctcggccg cacgccatgg
      181 atccagccgc acaccgacgt gctgctcgac gagctcgcca agcaaggcaa gaagcgcctc
      241 gcggtgctgt gcccggcgtt cgtcgccgac tgcctcgaga cgctcgagga gatcgggatc
      301 cgcgcgcgcg agcagttcaa ggccgccggt ggcgaggagc tcacgctcgt gccgtcgctc
      361 aacgcgacgc cggcgtgggt cgatgcggtg tgcgcgatgg cggagcgcca cgccgcgcgc
      421 aaacagctac cggtcgttac gtagacctct agatcagctc gagacacgcg cggacgtaac
      481 gcgctgcgag ccgcgtcgtg gcgtcggtgg cctcggccgt cgggccgagt ggcggcgcga
      541 cctcgacgca gtccgcggcg cagaggccga tgtccttgcc gatgcgatgg atcagcgcga
      601 acagccagtc gggctcaagg ccgtcgggct cgggcgtgcc agttgcctcg gcgtgtttcg
      661 cgtcggtgcc gtcgatgtcg ttcgagaagt agacgctcgt gacgcccttc gcgcgcagat
      721 gatcgatcac cgcgtcgagc gcagcgtcgg gatcgcggag gatctcggcg gcccagaact
      781 gccgcacgcc gagcgtgctc tcccagtgct cgcgcccaaa gcgcgatgca cgcacgccga
      841 cctgtacgag ccggccgtcg cggccgagca gctcgttcgc gtgatagctc caggtcgcgt
      901 agcagtactt gatgccgagc cgctcggcga gcagatcggt gtgcgcgtcg ggctgcacga
      961 tgccccagcg atcgcggcga tgcttcgcga gcgtctccga gaccggccac gcgaccgagt
     1021 gatcgccacc gagcacgatc ggaatcacgc gcggattgat cgcgagcacg caatcgagca
     1081 cgcgcgcttc gatcgatagc ggcgagacgg gaagctcagc ggcgagaccc ggcgcgacat
     1141 ctttatagag cgcggcgcga ctcgcttcga tctgtgcggg tgcgagcatc gcgtcgtgca
     1201 gcagctgcgg cacgaccgcg acatcgccga tatcgactat cgagcggcgg tcgagccaac
     1261 cgcgcaggtc gggatcggca gcgatcagag cacttctgat cgcctggggg ccgaggttgg
     1321 cgccgcggcg gtagccggca cctacatcag aggggacgcc caggatgatc gccttggcat
     1381 ttgcgattcg atcgagcgcg gcgatgaagc ggttttggat cgaagcctcg tcggaaacgc
     1441 catatagatg acgctgcact tcgagctgtg ccgcgcggcc ggtcgagacc acatagaggc
     1501 caccggcgga cggtcggagc atcgcctgaa gctgggcgcg agggtcggac acgagggcac
     1561 cctaccccag cgcgtgccga agatcccgca actaagttgc cgcaacggct ggaaatcagg
     1621 ttccatcgcg gagtatcaac ggtacgtcaa attcaaacct ttgaccggag actccatgaa
     1681 tcgcctattc aggcccacta tcgtggccgc gctgggaatc gcggcttcag cgtcgatcgc
     1741 tagcgccaac gtggagatcg gcggtactgc cggtattcac gtattcagca cggacaacga
     1801 gctcggtgtt cccgacaccg acgatgctcc gtccgagcgc aactcagcgc tgttcggtct
     1861 tcgcctcggc gtgacgttca acgacatgct gggcgtcgaa ggcgagttcg gtgtcattcc
     1921 gagcgaatca cgcgagctcg tgttcgacgt ttggaacctg acctatcgcg ctcacctcat
     1981 cgcgcagttc cgtgcggcga atccggcgac gaagctggtt ccgttcgtcg tgttcggcgg
     2041 cggcgcgatg gcggtcgtcg actcgaagaa cgaagtgaag atcgacaagg acaccgacgc
     2101 gatgctctac gcgggcgtcg gtgcgaagta ccgcgtcgac aacggctggg gtctgcgtgc
     2161 tgacgcgcgc atcctgttcc cgccgagctc ggccggtaac ggtccgacgg tcgacttcga
     2221 agcgctgctc tcgatctaca aagagttcgg ccgcaaggcc gccgagaagg ttgtcgaaca
     2281 gccgaagggc ccgaacgaca ccgatggcga cggtctgacg gacgacgtcg acaagtgccc
     2341 gaacgaagcc gaggacaagg acggcttcca ggacgacgac ggttgcccgg ataacgacaa
     2401 cgatggcgac ggtgtccccg acgccagcga caagtgcgcg atggagccgg aagacaagga
     2461 cggcttcgaa gacgacgacg gctgccccga agcggataac gacaaggacg gcatccccga
     2521 cgccagcgac aagtgcccga acgaagccga ggacaaggac ggcttccagg atgacgacgg
     2581 ctgcccggat ccggacaacg acggtgatgg cgtgccggat ggcagcgaca agtgccccga
     2641 tcagccggag accaagaacg gctatcagga cgacgacggt tgccccgacg agattccgca
     2701 gaaggtgaag cagttcaccg gcgtgatcca gggcatcaac ttcaaggtga acgatgcggc
     2761 cctgctgccg gcgtcgaaca agacgctcga caaggccgtc gcggtcctca aggagttcgg
     2821 cgacctcaag ctcgagatcc aaggccacac cgacgaccag ccgatcaagg cgggcggcaa
     2881 gttcgccgac aacaaggcgc tctcgcaagc gcgcgccgag acggtgaagg cgtacttcgt
     2941 cagcaagggc atcgaagagg gtcgcctcac ggcggtcggc ttcggtgacg agcagccggt
     3001 ggttgcgccg acgggcctca agggagcgaa gctgaactct gcgcgcgcga agaaccgccg
     3061 cgtcgagttc aagctcgtca gcacgctggt gccgggtggc ggagcggctc agggtggcgg
     3121 cggcgcgatg ccgcctcctc cgtgagccac gcgcactgag gaacctccac tgaggtgaag
     3181 acgaggcctt cgggcctcgt ttttttttcc cgcgggatac gctcaggcca aggtgagcgc
     3241 ggtcgcaaac gaccgcgctc tcgatccctt gccttcgcta tcggccaccg gctacgaaca
     3301 gcaaatggct ctctcgcgcg agctcctcga ctggctggtc tgtccgaagt ccaagcaacc
     3361 gctcgtgtac ttcgagaaag agggcttctt gctctgtccg gagtcgcggc tccgctaccg
     3421 gatcgagaac gacgtaccgg tgctcctcgt agaggaagcg actgaagttt ctgggccaga
     3481 gatcgagcgc ttgctcaaac aagccaagga gctaggcctt ccaggcgctt agctccaacg
     3541 ccatgcttga cctttggcgt caataacggt agatcgccgg ttccttcacc ggagcccgcg
     3601 gtgctgaagt cgctatcgat cttcactggt aacagtaatc gcgcactcgc agatgaaatc
     3661 tgcaagttcg tagaaattcc gcttggccgc gcggacgtca ctcatttcag tgacggcgaa
     3721 atctacgtcg aaatcggcga gaacgtccgc ggcgtcaact gcttcgtcgt gcagcccacg
     3781 tgctcgccgc cgaaccaggc gctgatggag ctcttgatca tgatcgacgc gctcaagcgt
     3841 gcgtcggccg gatccatcgt cgcgatcgtg ccgtacttcg ggtacgcgcg gcaggatcgc
     3901 aaagcgaagc cgcgcacgcc gatcaccgcg aagctcgtcg cgaacctgat caccgcggca
     3961 ggcgccgacc gcgcgcttgc gatggatctc cacgccggtc agatccaggg cttcttcgac
     4021 attccgttcg atcacctgtt cgcgatgccg gtcctcatcg aggagcttcg cgtgctcggc
     4081 tttggcggcg accagacggt cgtcgtgtcg cccgatgcag gtggtgtcga gcgcgctcgc
     4141 gcattcagca agcgcctcgg tgcgagcctc gcgatcatcg acaagcgccg tccggcgccg
     4201 aacgtcagcg aagtgatgaa catcgtcggc gacgtcaagg gcaagaaggc cgtcatcgtc
     4261 gacgacatca tcgacaccgc cggcacgctc acgcacgccg cggcagcgat cgtgaacgcg
     4321 ggcgcagcga gcgtctcggc gtgtgcgagc cacgctgtgt tctcgggcaa ggcagtgccg
     4381 cgcatcaccg atagtgcact gcagcacgtg atcgtcacca acaccattcc gctcaacgaa
     4441 gcgggtcaac actgcgagaa ggtgcgcgtg aagagcgtcg ggcgtttgct cggcgaagcg
     4501 atcaagcgca tccaccacgg agactcgatc tccagtttgt tcgtctgaaa gaagtctgaa
     4561 gggaacctgt catgccggaa gctcaagtcg gaaagctcac tgtcacgctg cgtaacaact
     4621 ctggtaaggg cgctgcgatc aagttgcgcc agcagggcaa ggttcctggc gtttgctacg
     4681 gcgcatcgcc ggaaggtcgc atcgatccga tgccgatcgt cgtcgacgtc aaggcgctcc
     4741 gcgccgcgct cgacccggtc cgcaagcaga acacggtcat cgatctgacg atcgatggcg
     4801 gctcggcacc acgcaagctg cacgcgctcg tgaaggagta ccagctccac ccgctgcgtc
     4861 gcgaaatcac gcacgtcgac ctgctcgcga tcgatccgaa caaggaagtg tcggcgcagg
     4921 ttccgctcga gttcgtcggc aagccggcgg gcgcgatcga cggcggtcag attcgcatcg
     4981 tcatccgcgc gctcgaagtc aaagcgaagc cgagcgacat tccggtcaag ctcacgatcg
     5041 atgtctcgcc gctcgagatc ggcgacgtca tccacgtcaa cgcgatcgcg ctgccgagcg
     5101 gcgtcaccgc gctgaccggt cgcgacctcg ccgtcgtgac ctgcgcgaag ccggaagagg
     5161 agaaggtcga agcggcggcg gtcacgcccg aggcggctgc ggctgcagcg gctggtggcg
     5221 cggctccggc ggctggcgcg gcggcggctg gcgcggctcc ggcggctggc gcgaaggacg
     5281 ccaaggcggc ggctccagcg gctggtgcga aggacgcgaa ggcggccgcg gctccggcca
     5341 agaagaagta atcgcgaacg catcgtcgcg agccttcggg cgcgcaacga cgcacgcgag
     5401 atggcgccgg gcacgttgcc ggcgccgtcg tcgtttcggc ggtgctcgtc gcgtttactt
     5461 cgcgccacgg ggccaacgcg ctataccccg tctccttcta tgacctggct cgtcgtcggg
     5521 ctcggcaatc cgggcagcaa gtacgaacgc aatcgccaca acatcggctt tcgcgtcgtg
     5581 gacgagctcg tccgcaagca cggcctgccg gcgtggaaga gcgcgatcaa gggcggcgag
     5641 tcgacgagcg gcatgatcac gaccgaacgc ggccgcgaga aagcgttcgt gctcaagccg
     5701 ctcgagttca tgaacctgtc tggcttcgcg acccagcgcg ccgcggcgtt tcacgacgtc
     5761 gacatcacga acatcctcgt cgtgcacgac gagatcgatc tcgacttcgg catcgtccgg
     5821 ctcaagaacg gcggcggcca cggcggccac aacggcctgc gctcgatcca ggagcagcta
     5881 ggcggtggcg gctacgcgag gctgcgcatg ggcgtcggcc gcgacccgaa caagccgccg
     5941 ggcagcaagg acgccgcgaa ctgggttctg gcggatttcc cgagcgctca ccaggcgacg
     6001 gtcgacgcca tgatctcagc cggttgcgag tgcatcgaga ccacgctcgc gcttggcatc
     6061 gtggccgcga tgaacaaaca caacgctcgt cccaacctcg caacttcgtg atatagaccc
     6121 cgcctctctc gctcttcccc cgccacggaa gggctttaca cccacgagga gtcacatggc
     6181 acgactgcag tcactacgag acacgccgaa cacggcgcgc gaatacgaaa cgatctacat
     6241 cctgcgcccc aacacgccga acgaaggcgt cgcggaggtc aacactcgca tcaagaacgt
     6301 gatcgagagc atgggtggca aggtcatcaa ggtcgacaac tggggcaagc gccgccttgc
     6361 ctatgaagtc gccaaagagc gcaagggcat ctacctgtac tggcagtacc tggcgcagcc
     6421 gggcgtcgtc gaggagtgcg agcgtaacct ccgcatgctc gacaacgtga tccgctacct
     6481 gacgacgaag gtcgacgaga acatcgacgt caccgcgcgt ccgaccgaga tcgacgactc
     6541 gtcgtacgag aaggcggcgc agaccgcggc cgacgaagaa gacttgttcc tctcgcgtgg
     6601 ctcggaagag ctgccgtcta gcgacgagga cgacgagttc ggccaggacg attttaacga
     6661 agagccgagc tcgggtggca tgtccggcgg cctcggggag aaggagtaaa caatgtcgac
     6721 tcgcatggac gctggtgatg aaggtctcga tcgcgacggt gatgaccgcc gcggtggcaa
     6781 gggtcgcggc gcagcacgcc gcaagctccc ctggcccatg gaccagatcg tcatgatccc
     6841 caccctgcgc ctccaatcga ccagcgtcgg caccgaactg cacgccgacg aagcgtggaa
     6901 gggcgtggat tttcagttct ggcaggtccg cggcaatacc gccagcgata acgacatgga
     6961 agataacttc gtcaccggcg agaggggcgc tccccaaatc atcgaaacct ttg
//
LOCUS       DASUFX010000831         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2081869, whole genome shotgun sequence.
ACCESSION   DASUFX010000831 DASUFX010000000
VERSION     DASUFX010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081869"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFV99_33475"
     CDS             <1..493
                     /locus_tag="VFV99_33475"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064350.1"
                     /translation="CADCHGTYARDGEPISYDEPIVPLADIGTDPARAHAATESFARA
                     ANDRTLNRGFTKFRYTDGYVPPVLTNVWARAPYGHAGQWPSLAVLAMAPEQRPTSFVV
                     DLDAPYDLATVGVPMRAPGGSLGAGEYLHDATKPGLSVAGHPFLAELGNDAPAVIEYL
                     KAL"
     gene            complement(512..1024)
                     /locus_tag="VFV99_33480"
     CDS             complement(512..1024)
                     /locus_tag="VFV99_33480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064351.1"
                     /translation="MRGWFCLFVLAACTGEPGGSRPTIDVPPLEIGGDTEVTHEPGEC
                     TYNVALYGMTGNANATPVGPITVDTSGIDICLSLDARDNLVVAHFAAGTQYETAASSS
                     FELALYDGDGTLLRDGWDVAFGSTPTTAFANLEYGVTKGQVMDAVLWVRAKAGTATST
                     VNVTLFEPYE"
     gene            complement(1069..>2049)
                     /locus_tag="VFV99_33485"
     CDS             complement(1069..>2049)
                     /locus_tag="VFV99_33485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064352.1"
                     /translation="DACMFGAPGALGYDACKKGAVCGNYRGGTGECKQICDQQGGQPA
                     CDAQHVCVTYSGLFSTGDTTPAAGGVCDLACDPLADNDFDGSGTLVKTGTTCGSSASN
                     GCYGYPSRGTALPTGWSCGADVNVAESQPAGLRHRVQCTTTNNCADPGPVIYVNSCNQ
                     GYLPLLRESTMVSTAICTALCQPKNCFMGSCGGGNNPDRLGAAPHRCQVPDRMGSFDT
                     TAGGESCRYIWSFEIDDQGNFLRSPTSDTVGFCFDHGKYQYDSDGDNTPDTPLPACAT
                     LPDGFGSGSAFGAADLGCVDTSHAVGLAQGKLMHRVFDDLRPLTRPQMAQ"
BASE COUNT          332 a          700 c          684 g          333 t
ORIGIN      
        1 ctgcgcggat tgccatggca cgtacgcgcg cgacggcgag ccgattagct acgacgagcc
       61 gatcgtgccg ctcgccgata tcggcacgga cccagcgcgc gcgcacgccg cgaccgagag
      121 cttcgcgcgc gccgcgaacg atcgcacgct gaaccgcggc ttcacgaagt tccgctacac
      181 cgatggctac gtgccgccgg tgctgacgaa cgtgtgggcg cgcgcgccgt atggccacgc
      241 cggccaatgg ccgagcctcg cggtgctcgc gatggcgccc gagcagcggc cgacctcgtt
      301 cgtcgtcgat ctcgacgcgc cgtacgatct cgcgaccgtc ggcgtgccga tgcgcgcgcc
      361 cggcggctcg ctcggtgccg gcgaatatct gcacgatgcg accaaacccg ggctctcggt
      421 tgcgggccat ccgttcctcg ccgagctcgg caatgatgcg cccgcggtga tcgagtacct
      481 caaagcgcta tgaagctcgc ggcacgagcg atcactcgta gggctcgaac aacgtcacgt
      541 ttaccgtcga tgtcgccgtg cctgccttcg cacgcaccca cagcacggcg tccatcacct
      601 ggcctttcgt gacgccgtac tcgaggttcg cgaacgcggt cgtcggcgtg ctgccgaacg
      661 cgacgtccca gccatcacgg agcagcgtgc catcgccatc gtagagcgcg agctcgaacg
      721 acgagctcgc agcggtctcg tactgggtgc ccgccgcgaa gtgcgcgacg acgaggttgt
      781 cgcgcgcatc gagcgacagg cagatgtcga tgccactggt gtcgacggtg atcgggccga
      841 ccggtgtcgc gtttgcgttg ccggtcatgc cgtacagcgc gacgttgtac gtgcactcgc
      901 cgggctcgtg cgtcacctcg gtgtcgccgc cgatctcgag cggcggaacg tcgatagtcg
      961 gccgcgagcc accgggttca ccggtgcaag cggcaagcac aaacaggcag aaccatcccc
     1021 gcatgcacac acagtggtga catgcgggga aaaagtcgcg cgcgatcgtt actgcgccat
     1081 ctgaggcctg gtcagcggac gcagatcgtc gaacacgcga tgcatcagct tgccctgcgc
     1141 gagcccgact gcatgcgagg tgtcgacgca gccgaggtcg gctgcaccga acgcgctgcc
     1201 cgagccgaag ccatccggca gcgtcgcgca tgccgggagc ggcgtgtccg gcgtgttgtc
     1261 accgtccgag tcgtattgat acttgccgtg atcgaagcag aagccaacgg tgtcgctcgt
     1321 cggcgagcgc aggaagttgc cttggtcgtc gatctcgaag ctccagatat agcggcagct
     1381 ctcgccgccc gcggtcgtat cgaacgagcc catgcgatcg ggcacctggc agcgatgcgg
     1441 cgccgcaccg aggcggtcgg ggttgttgcc gccgccgcaa ctgcccatga agcagttctt
     1501 cggctggcag agcgcggtgc agatcgccgt cgacaccatc gtgctctcgc gaagcagcgg
     1561 cagataaccc tggttgcagc tgttcacgta gatcacgggg ccggggtcgg cgcagttgtt
     1621 cgtcgtcgtg cactgcacgc gatggcgcaa gccagctggc tgcgactcgg cgacgttgac
     1681 gtcggcgccg cagctccagc cggtcggcag cgccgtgcca cggctcggat agccgtagca
     1741 gccgttgctt gccgagctac cgcacgtcgt gcccgtcttg accagcgtgc ccgagccgtc
     1801 gaagtcgttg tcggcgagcg gatcgcacgc gagatcacaa acgccgccgg ccgcgggcgt
     1861 cgtgtcgccg gtactgaaca agcccgagta cgtcacgcac acgtgctgcg catcgcatgc
     1921 gggctggcca ccctgctgat cgcagatctg tttgcactcg ccggtgccgc cgcggtagtt
     1981 gccgcacact gcgcccttct tgcaggcgtc gtagcccaac gctccgggag caccgaacat
     2041 gcaggcgtc
//
LOCUS       DASUFX010000832        13449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1524278, whole genome shotgun sequence.
ACCESSION   DASUFX010000832 DASUFX010000000
VERSION     DASUFX010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524278"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFV99_33490"
     CDS             <1..1344
                     /locus_tag="VFV99_33490"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX5064353.1"
                     /translation="SVHYSSNYCNAYWNGQQMVYGDGSTSQGCKPLARSVDVTAHELT
                     HAVTENESGLVYSGESGGINESLSDIFGTFTEAWTDGGKNGTLAISSDTWLLGEDVIS
                     GGLRFMCDPAKDGASKDFWTSSVGSVDVHYSSGVGNLEFCLLTKGGTHPRGKSNINVP
                     ALGMDKAIRIYYKAATDILTSSSKYANLRTAMEQAATALGYDQATKDAVSCSWAAVGV
                     GSAPTNCGGTTPPPPPPTGDGVLMNNVPVTSISDAKGGNKYWSFAVPSGQTTMTITIN
                     GGSGDADLYVQFGTKPTASSYACRPYKNGNAETCTFTPPQAGTYYVMLNAYAAYSGVT
                     LTGSYSASGGGGDPYLSNGVAVTGLSGATSSAAYWRVAAPAGKTLTIKISGGSGDADL
                     YTKFGSRPTTSSYLCRPYLNGNSETCTTTNTTAGDYYVMLRGYTSYSGVSLIASY"
     gene            complement(1473..2888)
                     /locus_tag="VFV99_33495"
     CDS             complement(1473..2888)
                     /locus_tag="VFV99_33495"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5064354.1"
                     /translation="MGRITVLMFLAACAHETSTPPEPQSSPPPPPLRVVFGTCAPETM
                     QFIVGATARPRVTGQVGDAFLCDGQPCGVIGAGSGIGTLSGGHGTPSQGYGISYGYIG
                     HGSGPHYASVPSVKQGALTALGELDQTIVRRYIRRNINRIRYCYEKELVTKSDLAGTL
                     TASWTIDSGGLVRDVKLDGFPAVASCMTTALSAIEFPRPKQGSVKASTSFTFTPGEGA
                     TYARPPHDPVAPPPPENPLRTIEPALTACLHTQPDHYGTGLVEFGAQPKVIGLAQPAC
                     VEKLVAELNKTAKPMRCSFAFGEMPFAKLPTLELQASATFFNGEDRANAKQAIEQHLV
                     ETHDAPLAMQGPIAITAQPTTPMTIVRDAAVAIDDADLDVAYVAPHAFGGVRLVPALE
                     LPPAGLPKRPRADQPGPVAELTVTQADVANWNALRAKLRALRTNSAGELLLHADTLQY
                     QDFVRVVDLAGEEGFAQWRLR"
     gene            2926..3708
                     /locus_tag="VFV99_33500"
     CDS             2926..3708
                     /locus_tag="VFV99_33500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5064355.1"
                     /translation="MRRMRLIAIAVMLVACKPKPPNKPDPRVAQLEKELADTKAALDK
                     EHQDAEALRSSDAELKAKADEAAALEAKLAEAVGKAGEVTTADGAVKLELVDQILFPV
                     GEADLTPRGKEVLAKVGAALKDINDKQIWVQGHTDDQPILVKKPKAEPPKPEPKKAAA
                     KPPKKGAKDAKPDPKEVKAEAKPEEKPAETNDAVLPFVTNWELSAARALTVVHYLQDE
                     AKIDPTRLAAVAFSQYRPVSKNRAKNRRIEIVLYPRAKISPK"
     gene            complement(3709..5244)
                     /locus_tag="VFV99_33505"
     CDS             complement(3709..5244)
                     /locus_tag="VFV99_33505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX5064356.1"
                     /translation="MTSLGEPNVIAIVSFIGFVLITLGITYWAARRTRTSAEFFAAGG
                     SVSAAQNGFALAGDYMSAASFLGIAGLVSKDGFDGLIYSIGFLVGWPLITFLIAEPLR
                     NLGTYTFVDVVAFRLRQRPVRIAAAISTLTVVSFYLIAQMVGAGGLVHLMFGLPYELA
                     VVVVGVVMLIYVLFGGMIATTWVQIIKAGLLLLGATLLAVLVLAQFNFNPLALFGEAV
                     KHDGAAVMAPGKLVANPLEAISLGLALMLGTAGLPHILMRFYTVKDAKTARRSVAYAT
                     SLIGFFYLVTFVLGFGAMVLVGRKTIIAVDKGGNMAAPLLAEVLGGTGFLGFISAVAF
                     ATILAVVAGLTLSGAAALAHDLWGHVLRDGKVEEREQLRVAKIATIGLAVVAIGLGLL
                     FKGQNVAFMVGLAFSIAASANFPALLMSMTWSRFTSLGAMTSMLTGTIVSLVLILLSP
                     TVWVDLFKNAEPIISLKNPGIFSVPAAFIVGIAVSLLKPEPASVQGFRAAQDRMHLGP
                     RPR"
     gene            complement(5241..5525)
                     /locus_tag="VFV99_33510"
     CDS             complement(5241..5525)
                     /locus_tag="VFV99_33510"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX5064357.1"
                     /translation="MDEDVEKLVARRWRVGSVLTAVMVTAYFGFILLVAYDKDTTGAL
                     IAGDRVSIGIVLGAALIVLAPILIAIYARWANRSYDPQVEALRKKRDGAP"
     gene            complement(5541..6698)
                     /locus_tag="VFV99_33515"
     CDS             complement(5541..6698)
                     /locus_tag="VFV99_33515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5064358.1"
                     /translation="MRSFAILLAVLAACRGSARSTLPPPPPSSATPPTQVGPAPNITW
                     KKVDSPDDVPREPPPAGTYRVHLIDVGTGLAILVQGHDFNLLYDGGTNDRDEKPLRVI
                     DYLARAIGPSGDDLCVDKGDPAPTQRLPLDHVVLSHPHLDHGSALDLVVHCYDVKNVW
                     DSGRVNDAAFYRDFIEAVAASTTVVYHTAASVPVDHVVGVKGAEIKIGRWERFSEDDT
                     VPLGEAAKFTILHAEAKKLSDPNQNSVVITVELGTTHVLLVGDAESGARKDPSDPPGD
                     VEEFLLQHHATAVRADILQVGHHGSKTSSRRAFLAAVKPHIALVSSGPKLYGKVVLPD
                     PEVIDALKSLGAQILRTDERDVSCPLEHRIGGDRGPGGCDSYVITIEPPAR"
     gene            complement(6695..7489)
                     /locus_tag="VFV99_33520"
     CDS             complement(6695..7489)
                     /locus_tag="VFV99_33520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5064359.1"
                     /translation="MRRPTKSPLGEAIARVIEDRDCFCVFQPLVDLRTQKVFAYEALA
                     RTKAREFEGPMQMFAAAVSENLTGELGRMLRSMALEGCQGHPLFLNVHPAELNERWVV
                     QPDDPIFQHSEDVYLEITEAVPLSHFRLCSDMLAEVRGRGVHLVVDDLGAGYSNLKYI
                     ADLHPRVVKLDRGLIAGLTKDTRLFKLVSAIVRMCSELDSRVVAEGIETETELEAVIA
                     AGARYGQGYLLARPAFPPPQVTWPASVMPSPIASSHRLDDATRKRA"
     gene            complement(7516..8154)
                     /locus_tag="VFV99_33525"
     CDS             complement(7516..8154)
                     /locus_tag="VFV99_33525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064360.1"
                     /translation="MRAVIALLLLTGTAYAGNNEISYTSTNRALRSDSANALTGDSLF
                     GGGLAYGRRINTLAGLEMWVNGTFGWGTASGEMFQTMTTQLSTLWLTAGAQLRYHLTE
                     RRFVASGRLDVGSARASLAIHDDMGHTASDHGWGAISSAALGIDCYAFAGQRYALSFR
                     AELSAVATSSIPLTATPDATSDDTLHLEMTAASLGGLNLSGPAFSVSAVFQF"
     gene            complement(8156..9673)
                     /locus_tag="VFV99_33530"
     CDS             complement(8156..9673)
                     /locus_tag="VFV99_33530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064361.1"
                     /translation="MWDRERTVLGPIPMKSQIAYVDSALDRVTLLDLGKDEPKITTEK
                     IGRRAVVAVPSPDRHLLFVITRGEEAIVKGQIDQPPMFWVVDTVNPEAETRGYEIGSP
                     FDRIAVAPDNSMVVAYFSAAGPDEAGFFRNPNELAVIHLNEPPSDLNPTLKTIRSFGS
                     VPQGVTLSPPMVVPGAEDPSPRTFAFVLSSNNLTLIDANNPTRREVSVRLDLGGQAVI
                     PREVVFAPNTSSAYVRSDNARDVLQVLIQAVPPETPEHSDFKPLLAELGAGGGPTDIA
                     VYDDPSNRRYVLASTPNTGEIVVIDADTAQFRSVPVSDPIDRILLFPSGGGVPPTKAL
                     FASVGSKVTRVHVLDLVDIQDPLTQAKLHTINLSYPVRDVAPVPGRDLAMLVHDDART
                     VLGLLDMDTLSTSPLLGVGKLDSYDFSPSGSYLIGATDAVSRVGFVALDNLHPTDFRL
                     DDPPARVLSTANAKVFVDHGDPLGHATIIPSPTATREQALVLTGFLTNDLLDEGN"
     gene            complement(9748..11466)
                     /locus_tag="VFV99_33535"
     CDS             complement(9748..11466)
                     /locus_tag="VFV99_33535"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX5064362.1"
                     /translation="MAARHIHCHRVGSEDPMRVLCCLGGLIVAAACSSEVAGDYGGSL
                     VDGGASADSGYVGCLSSNECPAGYVCNDFGRCERPPSTDAGVMPPPEVEYDFGAPISS
                     QRYVYVAMTAEDSLARIDGTTLAVRSTKVGESPREVATIPGTDGAVVLDTSNGTATIV
                     RPDGDSDAIRVLGTLQRLNRLDIDPSGRFAVIWFDLAKAIAEGNITNVGSFQDVTVLA
                     LAPGAEKAVNLTVGFRPRNVQFDAAGNRAYVVTEDGVSVIDLAYATDHEPSIVPPIPV
                     IDPSVPASDVEVDIVATGEYAAVRQSGASSLRIVNVGSMPGVAFQIPLASPPTDIDLA
                     PDGARLYAVLRSAKQLAIIDVPGDAQNPTGVETVDLTNATLGSLVLSRDGHRGLLYTN
                     ATADERITLVKLDQPGYPKQTWTLKKSVRAVGISPTGDTALLLNAKAFGDPSTASNFD
                     DYIDKSYGYTLLDLATGFGKLQITPVDPGPFTYSPDGSKVYVNLDGGDTVNATRAIQV
                     VTTQTGVVITKPLGSPPSSVGMLPGAAQAFVAQRHPLGRVSFLDLTSDAVRTVTGFDL
                     NSQVVQ"
     gene            11600..12685
                     /locus_tag="VFV99_33540"
     CDS             11600..12685
                     /locus_tag="VFV99_33540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5064363.1"
                     /translation="MSSDPVLSVLPLGFPWQTFDPFLFCVHHDDAYPAGNEHMGPAAS
                     LAGRDIGQDFAGKDGWRMYHGETVPGFPQHPHRGFETVTIVRRGLIDHSDSLGAAARF
                     GRGDVQWLTAGKGIVHSEMFPLLDKSKPNPVELFQIWLNLPRAGKMVNPYFSMLWDHT
                     IPRQEARDDAGRTTEITVIAGTVGDAKAPAPPPDSWAARADADVAIWSIRMMPGATWT
                     LPATRASTNRTLYFFRGTSVQIASRKLEKNAGVRLRPDADVTVVAGPDGAELLLLQGR
                     PINEPVVQYGPFVMNTPAEIQQAFMDYRRTQFGGWPWPKDDPVHGREDVRFARHADGK
                     IERADGGTADPSGHSSMKPLGSSTGSV"
     gene            complement(12640..>13449)
                     /locus_tag="VFV99_33545"
     CDS             complement(12640..>13449)
                     /locus_tag="VFV99_33545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064364.1"
                     /translation="VVEVMDVLRDAHRNAVVNMLGVAVEHRPEFFAQLLPPLVELRTR
                     TGRPHWLVVDEAHHLLPGSWEPATELALRPRGTIYVTVHPGSVAKPVIESINTLLVVG
                     DHPDKTVKEFAKVAAVKKTPHVPTEKLAAGHTLLWHTDSKQAFDVQTYQCRTERTRHS
                     RKYVEGNLGPHSFYFRGPDRRLNLKASNLMLFVMLAEGVDDDTWTYHLRKREYSTWFR
                     DNVKDPELAEAVERVETDTKLSAEASRMVIRGEITKRYTLPVDEPSGFIDE"
BASE COUNT         2451 a         4297 c         4513 g         2188 t
ORIGIN      
        1 tccgtccact actcgagcaa ctactgcaac gcgtactgga acggtcagca gatggtgtac
       61 ggcgacggca gcacgtcgca aggttgcaag ccgctcgcgc gctccgtcga cgtgacggcg
      121 cacgagctca cgcacgcggt caccgagaac gagtcgggcc tcgtgtacag cggcgagtcg
      181 ggcggcatca acgagtcgct ctccgacatc ttcggcacgt tcacggaagc gtggaccgac
      241 ggcggcaaga acggcacgct cgcaatttcg agcgacacgt ggctgctcgg cgaagacgtc
      301 atctcgggtg gcctccgctt catgtgtgac cccgcgaagg atggcgcgtc gaaggacttc
      361 tggacgagca gcgtcggctc ggtcgacgtg cactacagct cgggtgtcgg caacctcgag
      421 ttctgcctcc tcacgaaggg cggcacgcac ccgcgcggca agtcgaacat caacgttccc
      481 gcgctcggca tggataaggc gatccgcatc tactacaagg cggcaaccga catcctgacg
      541 tcgagctcga agtacgcgaa cctccgcacg gcgatggagc aagcggcgac ggcgctcggt
      601 tacgaccagg cgacgaagga cgcggtgagc tgctcgtggg cagcagtcgg tgtcggctcg
      661 gcgccgacga actgtggtgg cacgacgcct ccgccgccgc cgccgacggg cgatggcgtg
      721 ctgatgaaca acgtgccggt cacgagcatc agcgacgcga agggtggcaa caagtactgg
      781 agcttcgcgg tgccgtcggg tcagacgacg atgacgatca cgatcaacgg cggctccggt
      841 gatgctgacc tctacgtcca gttcggcacc aagccgacgg cgtcgtcgta cgcatgccgc
      901 ccgtacaaga acggcaacgc agagacgtgc acgttcacgc caccgcaagc gggcacctac
      961 tacgtgatgc tgaacgccta cgctgcgtac tcgggtgtca cgctcaccgg tagctactcg
     1021 gcgtcgggtg gcggtggcga cccgtacctc agcaacggcg tggcggtcac cgggctctcc
     1081 ggtgcaacca gcagcgcggc gtactggcgc gtggcagcgc cggccggcaa gacgctgacg
     1141 atcaagatca gcggcggctc cggcgacgcg gacctctaca cgaagttcgg ctcgcgcccg
     1201 acgacgtcat cgtacctctg ccgtccgtac ctcaacggca acagcgagac ctgcacgacc
     1261 acgaacacga ctgccggcga ctactacgtc atgttgcgcg gctacacctc gtactcgggc
     1321 gtgagtctca tcgcgagtta ctaaagccac ggcgacgccc ggcagtgccg gagaacggag
     1381 gtctattccg acacacaaca aatcctctct caggatggtc acagaaacgc gctgagtgcc
     1441 tcggaaggct cagcgcgttc cccttttcgg gctcagcgca gccgccactg cgcgaagccc
     1501 tcttcgccgg cgagatcgac gacgcgcacg aagtcttgat actggagcgt gtcggcgtga
     1561 agcagcagct cgccggcgct attcgtgcgg agcgcgcgca gcttggcacg caacgcgttc
     1621 cagttcgcga catcggcctg cgtgacggtg agctcggcga ccggtccggg ttggtcagcg
     1681 cgcggccgct tcggcaatcc agcgggcggt aactcgagcg ccgggacgag tcgaacaccg
     1741 ccgaaagcgt ggggcgcgac gtacgcgacg tcgagatctg cgtcatcgat cgccactgcc
     1801 gcatcgcgca cgatcgtcat cggcgttgtc ggctgcgcgg tgatcgcgat cggtccttgc
     1861 atcgcgagcg gcgcatcgtg ggtctcgacg aggtgttgct cgatcgcctg cttcgcattg
     1921 gcgcgatcct caccgttgaa gaacgtcgca gacgcctgca gctcgagcgt cggcagcttc
     1981 gcaaacggca tctcgccgaa tgcgaacgag cagcgcatcg gtttcgcggt cttgttcagc
     2041 tcggcgacga gcttctcgac gcacgccggc tgcgcgagcc cgatgacctt tggttgcgca
     2101 ccgaactcga cgaggccggt cccgtagtgg tcgggctgtg tatgcaggca ggcggtgagc
     2161 gccggctcga tggtgcgcag cggattctcg ggcggcgggg gtgccacagg gtcgtgcggc
     2221 gggcgtgcgt acgttgcgcc ctcgcccggc gtgaacgtga acgacgtgct tgccttgacg
     2281 ctgccctgct tcggccgcgg aaactcgatc gcggacagcg cggtcgtcat gcagctcgcg
     2341 accgccggaa agccatcgag cttcacgtcg cgaacgagac cgccgctatc gatcgtccag
     2401 ctcgcggtca gcgtgcccgc gaggtcgctc ttggtgacga gctccttttc gtagcagtag
     2461 cgaatgcggt tgatgttgcg ccggatgtag cggcgcacga tcgtctgatc gagctcgcca
     2521 agggcggtca gcgcgccttg cttcacgctc ggaacgctcg cgtagtgcgg accagagccg
     2581 tgaccgatgt agccgtacga gatgccgtag ccctggcttg gcgtgccgtg tccgcctgac
     2641 agcgtaccga ttccgctgcc ggcgccgatg acaccgcacg gctggccgtc acagagaaat
     2701 gcgtcgccga cctggccagt gacgcgcgga cgcgcggtcg cgccgacgat gaactgcatg
     2761 gtttccggcg cacaggtgcc gaacacaacg cgcagcggcg gcggtggcgg cgatgattgc
     2821 ggctcgggag gtgtgctggt ttcgtgtgcg cacgctgcga ggaacatcag caccgtgatc
     2881 cgacccatcc ggagcgtgta gcacgatcag ggatacaatc gacgcatgcg tcggatgaga
     2941 ctaatcgcga tcgccgtgat gcttgtcgcc tgtaagccga agccgccgaa caaacccgac
     3001 ccgcgtgtcg cccagctcga gaaggagctc gccgatacga aggccgcgct cgacaaggag
     3061 catcaagacg cagaagcgct gcgctcgagc gacgccgagc tgaaggcgaa agccgacgaa
     3121 gcggccgcac tcgaagcgaa gctcgccgag gcagttggca aggcgggcga agtgacgacg
     3181 gccgacggcg cggtgaagct cgagctcgtc gaccagatct tgtttccagt cggcgaagcg
     3241 gacctcacgc cgcgtggcaa ggaagtgctc gcgaaggtcg gcgccgcgct caaggacatc
     3301 aacgacaagc agatctgggt gcagggccac accgacgatc aaccgatcct cgtgaagaag
     3361 ccgaaggccg agccgccgaa gcccgaaccg aagaaggccg ccgcgaaacc gccgaagaag
     3421 ggtgcgaagg acgcgaagcc cgatccgaaa gaggtgaagg cagaagcgaa gcccgaagag
     3481 aagcccgctg agacgaacga cgcggtgttg ccgttcgtga cgaactggga gctgtcggcg
     3541 gcacgcgcgc tcaccgtcgt gcactatctg caagacgagg cgaagatcga tccgacgcgg
     3601 ctcgccgcgg tcgcgttcag ccagtaccgg ccggtcagca agaaccgcgc gaagaaccgc
     3661 cggatcgaga tcgtgctgta tccgcgcgcg aagatctcgc cgaagtgatc aacgcgggcg
     3721 cggacctaag tgcatgcggt cttgcgcggc gcggaagccc tgcacggagg ccggctcggg
     3781 cttgagcagc gagaccgcga taccgacaat gaaggccgcc ggcacgctga agatgccggg
     3841 gttcttgagc gagatgatcg gctcggcgtt cttgaacaga tcgacccaga cggtcggcga
     3901 cagcaggatc agcacgagcg atacgatcgt gccggtcagc atgctggtca tcgcgccaag
     3961 tgacgtgaag cgagaccacg tcatcgacat cagcaacgcg gggaagttcg ccgacgctgc
     4021 gatcgagaac gcgaggccga ccatgaacgc gacgttctgg cccttgaaca agaggccgag
     4081 accgatcgcg accacggcga gaccgatcgt cgcaatcttc gcgacgcgca gctgctcgcg
     4141 ctcctcgacc ttgccgtcgc gcagcacgtg accccacagg tcgtgcgcga gcgcggcggc
     4201 gccggatagc gtgagacctg cgacgaccgc gaggatcgtc gcgaacgcga cggcggatat
     4261 gaaaccgagg aagccagtgc cgccaagaac ttcggcgagc agcggtgcgg ccatgttgcc
     4321 gcccttgtcg accgcgatga tcgtcttgcg gccgacgagc accatcgcgc cgaagccgag
     4381 cacgaacgtg acgagataga agaagccgat cagcgacgtc gcgtaggcga ccgagcggcg
     4441 cgccgtcttc gcatccttca cggtgtagaa gcgcatgagg atatgcggca gaccggcggt
     4501 gccgagcatc agcgcgaggc cgagcgagat cgcttcgagc ggattcgcga cgagcttgcc
     4561 gggcgccatc accgcggcgc cgtcgtgctt gaccgcttcg ccgaacaacg cgagcgggtt
     4621 gaaattgaac tgcgcgagca cgaggactgc gagcagcgtc gcgccgagca gcaagaggcc
     4681 cgccttgatg atctgaaccc aggtcgtcgc gatcatgccg ccgaacagca cgtagatcag
     4741 catcacgacg ccgacgacca cgaccgccag ctcgtacggc aggccgaaca tcaagtgcac
     4801 gaggccgcca gcgccgacca tctgcgcgat cagatagaac gacacgacgg tcagcgtgct
     4861 gatcgcggcg gcgatgcgca ccgggcgctg gcggaggcgg aacgcgacaa cgtcgacgaa
     4921 cgtgtatgta ccgaggttac gcagcggctc ggcgatcaag aacgtgatca gcggccagcc
     4981 aacgagaaaa ccgatcgaat agatgaggcc atcgaagccg tccttcgaga cgaggccggc
     5041 gatgccgagg aaggacgccg cgctcatata gtcgccggcg agcgcaaagc cgttctgtgc
     5101 cgccgagaca ctgccgcctg ccgcaaagaa ctcggccgag gtgcgcgtgc gacgcgccgc
     5161 ccagtacgtg atgccgagcg tgatcagcac gaaaccgatg aacgagacga tcgcgatgac
     5221 gtttggctcg ccgagcgagg tcacggcgca ccatcacgct tcttgcgcag cgcttcgact
     5281 tgcgggtcgt agctgcgatt tgcccaccgc gcgtagatcg cgatcaggat cggcgcgagc
     5341 acgatcagcg cagcgccgag cacgatgccg atgctgaccc gatcgcccgc gatcagcgcg
     5401 cctgtcgtgt ctttgtcgta cgcgaccagc aggatgaacc cgaagtaggc ggtgaccatg
     5461 accgcggtga gcacgctgcc gacgcgccaa cgccgcgcga ccagcttctc cacatcctcg
     5521 tccatgcgcg gactatagac tcagcgcgca ggcggctcga tcgtgatcac gtatgaatcg
     5581 cagccacccg gcccgcgatc gccaccgatg cgatgctcga gtggacaact tacgtcgcgc
     5641 tcatccgtgc gcaggatctg agcgccgaga ctcttcagcg catcgatcac ttccggatcg
     5701 ggcaacacga ccttgccgta cagcttcggt ccactcgata cgagcgcgat gtgcggcttc
     5761 acggcggcaa ggaacgcgcg ccgactcgac gtcttcgagc cgtggtggcc gacttgcaag
     5821 atgtcagcac gcaccgccgt cgcgtggtgc tggagcaaga actcctcgac gtcgcccggc
     5881 ggatcgctcg gatccttgcg ggcgcccgat tcggcgtcac cgacgagcag cacatgcgtc
     5941 gtgccgagct cgacggtgat cacgaccgag ttctgattcg gatccgagag cttcttcgcc
     6001 tcggcgtgca ggatcgtgaa cttcgccgcc tcgccgagcg gcacggtgtc gtcctcggag
     6061 aagcgctccc agcgtccgat cttgatctcc gcacctttca cgccgacgac gtggtcgacc
     6121 gggacactcg ccgcggtgtg atagacgacg gtcgtcgatg cagcgaccgc ttcgatgaag
     6181 tcgcggtaga acgcggcgtc gttgacccgt cccgagtccc acacgttctt gacgtcgtag
     6241 caatgcacga cgagatcgag cgcggagccg tgatcgaggt gagggtgact gagcacgacg
     6301 tgatcgagcg gcagccgctg cgtgggtgcg gggtcgccct tgtccacgca gaggtcgtcg
     6361 cccgagggac cgatcgcacg cgctagataa tcgatcacgc ggagcggctt ctcgtcgcga
     6421 tcattcgtcc cgccgtcgta gagcaagttg aagtcgtggc cctgcaccag gatcgcgagg
     6481 ccggtgccga catcgatcag gtggacgcga tacgtgcccg ctggcggtgg ctcgcgcggc
     6541 acgtcatcgg ggctatcgac cttcttccac gtgatgttcg gcgccgggcc gacctgtgtc
     6601 ggtggtgtcg ccgacgacgg cggcggcggc ggcaacgtgc tgcgcgcaga cccgcggcat
     6661 gcggcgagca ccgcgagcag gatcgcgaac gatctcatgc acgcttgcgc gtggcgtcgt
     6721 cgaggcgatg cgaactggcg atcggcgacg gcatcacgct cgcaggccag gtgacctgcg
     6781 gcggcgggaa tgcggggcgt gcgagcaaat agccttggcc gtagcgcgcg cctgctgcga
     6841 tcactgcctc gagctcggtc tcggtctcga tgccttcggc tactactctc gaatcgagct
     6901 cgctgcacat gcgcacgatc gcagatacca gcttgaagag gcgagtatct ttcgtcagtc
     6961 ctgcgatcaa tccgcgatcg agcttcacga cccgtggatg caggtcggcg atgtacttga
     7021 ggttcgagta gccggcgccg agatcgtcga cgaccagatg cacgccacgg ccgcgcacct
     7081 ccgcgagcat gtcggagcac agccggaagt gcgagagcgg cacggcttcg gtgatctcga
     7141 ggtagacgtc ttcggagtgc tggaaaatcg gatcgtcggg ctgcacgacc caccgttcgt
     7201 tcagctcggc gggatggacg ttgagaaaca gcggatgacc ctggcagcct tcgagcgcca
     7261 tcgagcgcag catgcggccg agctcgccgg tcaggttctc ggagacggcc gccgcgaaca
     7321 tctgcatcgg accttcgaac tcgcgcgcct tcgtgcgcgc tagcgcttcg tacgcgaaga
     7381 ctttctgcgt tcgcagatcg acgagcggtt gaaagacgca gaaacaatca cggtcctcga
     7441 tgacacgcgc gatcgcttcc cccagaggcg acttcgtagg tcttctcacc ggcagcgttg
     7501 atcataacgt agcgatcaaa actggaatac tgccgacacg ctgaacgccg gaccgctcaa
     7561 gttgagccct cctaacgaag ccgcagtcat ttcgagatga agcgtgtcgt cgctggtggc
     7621 atcgggagtc gcggttaggg gtatcgacga ggtagcgacg gcagaaagct cggcgcgaaa
     7681 cgacaatgcg taacgctgtc ccgcgaaagc gtagcagtcg ataccgagtg cagcgctgct
     7741 gatcgcaccc cagccgtggt cgctcgcggt gtgacccatg tcatcgtgaa tcgcgagtga
     7801 agcgcgcgcg ctgcccacat cgaggcggcc gcttgcgaca aatcgccgct ccgtcaggtg
     7861 atagcgcagc tgcgctcctg cggtgagcca cagcgtactg agctgtgtcg tcatcgtctg
     7921 aaacatctct ccgctcgcgg tgccccagcc gaaggtgccg ttcacccaca tctcgaggcc
     7981 ggcgagcgtg ttgatgcgcc ggccataggc gagcccaccg ccgaacaggc tatccccagt
     8041 gagcgcattt gccgaatcgg agcggagtgc gcggttcgtg ctcgtgtacg agatctcgtt
     8101 gttgcccgcg taggcagtac cagtaagcag cagcaaagcg atgacggcgc gcatgtcagt
     8161 tcccctcgtc gagcaggtcg ttggtaagga agccggtgag cacgagcgct tgttcgcgcg
     8221 tcgcggtcgg cgacggaatg atcgtcgcgt ggccgagcgg atcgccgtga tcgacgaaca
     8281 ccttcgcgtt cgcggtcgac agcacgcgcg ccggcggatc gtcgaggcgg aagtctgtcg
     8341 ggtgcaggtt gtcgagcgcg acgaagccga cgcgcgacac cgcatcggtc gcgccgatca
     8401 gatacgagcc gctcggcgag aagtcatacg agtcgagctt gccgacgcca agcagcggtg
     8461 acgtcgatag cgtgtccatg tcgagcaagc cgagcacggt gcgcgcgtcg tcgtggacga
     8521 gcattgcgag gtcgcggccc ggcaccggcg cgacgtcacg caccggataa gacaggttga
     8581 tcgtgtggag cttcgcctgg gtcagtggat cctggatgtc gacgaggtcg agcacgtgca
     8641 cgcgcgtgac cttcgagccg acagacgcaa acaacgcctt cgtcggtggg acgccgccac
     8701 cgctcgggaa caggaggatg cgatcgatcg gatcggacac cggcaccgag cggaactgcg
     8761 cggtatcggc atcgatgacg acgatctcgc cggtgttcgg cgtcgatgcg agcacgtagc
     8821 ggcgattcga cggatcatcg tagaccgcga tgtcggtcgg gccaccgccg gcaccgagct
     8881 cggcgagcag cggcttgaag tcggaatgct cgggcgtctc cggcggcacc gcctggatca
     8941 gcacctggag cacgtcgcgc gcgttgtcgc tgcgcacgta ggcgctcgag gtattcggcg
     9001 cgaacacgac ctcgcgggga atcacggctt ggccgccgag atcgagacgc accgagacct
     9061 cgcggcgcgt cgggttgttc gcgtcgatca gcgtgaggtt gttcgacgac agcacgaacg
     9121 cgaacgtgcg cggcgatgga tcttcggcac cgggcaccac catcggcggc gacagcgtga
     9181 cgccttgcgg gaccgagccg aacgagcgaa tcgtcttgag cgtcgggttg agatcgctcg
     9241 gcggctcgtt caagtgaatt actgcgagct cgttcgggtt gcggaagaag ccggcctcgt
     9301 cggggcctgc cgccgagaaa tacgcgacga ccatgctgtt gtcgggcgcg accgcgatgc
     9361 gatcgaacgg cgacccgatc tcgtagccac gcgtctccgc ctctgggttc acggtgtcga
     9421 cgacccagaa catcggcggc tgatcgatct ggcctttgac gatcgcttcc tcgccgcgcg
     9481 tgatcacgaa caagaggtgg cgatccgggc tcggcaccgc gaccacggcg cgccggccga
     9541 tcttctcggt cgtgatcttg ggctcgtcct taccgaggtc gagcagtgtg acgcgatcga
     9601 gcgccgagtc gacgtacgcg atctgcgact tcatcgggat cgggccgagc acggtgcgct
     9661 cgcgatccca catgatcgga cgctcgtctt cccaacacgc cccgagggca gcgatggcgg
     9721 acagagcaca aagggatgcg acgatcttca ttggaccacc tggctgttga ggtcgaagcc
     9781 ggtcacggtg cgaacggcgt cggacgtgag gtcgagaaac gagacgcggc cgagcggatg
     9841 gcgctgcgcg acgaacgcct gtgcggcacc gggcagcatg ccgaccgacg acggcggaga
     9901 gccgagtggc ttcgtgatca cgacgccggt ctgcgtcgtg acgacctgga tcgcgcgcgt
     9961 cgcgttgacg gtgtcaccac catcgaggtt gacgtagacc ttcgagccgt ccggcgaata
    10021 cgtgaacggg cctggatcga ccggcgtgat ctgcagcttg ccgaagcccg tcgcgaggtc
    10081 gagcagcgtg tagccgtagc tcttgtcgat gtagtcatcg aaattcgacg cggtgctcgg
    10141 atcgccaaat gccttcgcgt tgagcagcag cgcggtgtcg cccgtcggcg agatgccgac
    10201 cgcgcggacc gacttcttca atgtccaggt ctgcttcggg tagccgggct ggtcgagctt
    10261 gacgagcgtg atgcgctcgt cggccgtggc gttcgtgtag agcaggccgc ggtggccgtc
    10321 gcgcgacagc acgagcgacc cgagcgtcgc atttgtcagg tcgacggtct cgacgcccgt
    10381 cgggttctgc gcgtcgccag gaacatcgat gatcgcgagc tgtttcgcac tgcgcaatac
    10441 cgcgtagagg cgagcaccgt cgggtgcgag gtcgatgtcg gtcggcggtg acgcgagcgg
    10501 aatctggaac gcgacgcccg gcatcgagcc gacgttcacg atgcgcagcg acgacgcgcc
    10561 gctctggcgc accgccgcgt attcgccggt cgcgacgata tcgacctcga cgtcgctcgc
    10621 tggcacgctc ggatcgatga ccgggatcgg cggcacgatg ctcggctcgt gatcggtcgc
    10681 atacgcgaga tcgatcaccg acacgccgtc ctccgtgacg acgtacgcgc gattgccggc
    10741 cgcatcgaac tggacgttgc gcggccggaa tccgaccgtg aggttcacgg ccttctcggc
    10801 gccgggtgcg agcgccagga cggtcacgtc ctggaagcta ccgacgttgg tgatgttgcc
    10861 ctcggcgatc gctttcgcga gatcgaacca gatcaccgca aagcgcccac tgggatcgat
    10921 atcgagccgg ttcaaccgct gcagcgtgcc gagcacgcga atcgcgtcgc tgtcgccatc
    10981 aggccgcacg atcgtcgcgg ttccgttcga cgtgtcgagc acgaccgcgc cgtcggtacc
    11041 gggaatcgtc gcgacttcgc gcggagattc accgactttc gtggagcgaa ctgcgagcgt
    11101 cgtgccgtcg atccgcgcga gcgagtcctc cgcggtcatc gcgacataga cgtagcgttg
    11161 cgagctgatc ggcgcgccga agtcgtactc gacctcgggc ggcggcatca caccggcatc
    11221 tgtcgacggt gggcgctcgc atcggccgaa gtcattgcac acgtagccgg ccgggcattc
    11281 gttcgacgac aagcaaccga cgtagccgct gtcggccgac gcgccgccat cgaccaacga
    11341 gccgccgtag tcgccagcaa cttctgacga acaagcagcg gcgacgatca agccgcccag
    11401 gcaacacagt acgcgcatcg gatcctccga gccgacgcga tggcaatgga tgtgccgcgc
    11461 ggccatgtgc gatctcgttg cgttacgcgc cgcgacttgc cgcgccgcgc gaacacctct
    11521 gacacgaatg ccctagcaac agacctactc gctcggcgag attgctgtac gtcacattcg
    11581 ggcttatcga tcaatgacca tgtctagcga ccccgtgctg agcgtcttgc cgttgggatt
    11641 tccatggcag acgttcgatc cgttcctgtt ctgcgtgcat cacgacgacg cgtatcccgc
    11701 gggcaacgag cacatgggtc cggctgcgtc gctcgccggc cgtgacatcg gtcaggactt
    11761 cgccggcaaa gacggctggc gcatgtatca cggcgagaca gttccgggtt ttccgcagca
    11821 tccgcatcgc ggcttcgaga ccgtcacgat cgttcgtcgt gggctcatcg atcattccga
    11881 ttcgctcggt gccgccgcgc gcttcggtcg cggtgacgtg cagtggctca ccgccggtaa
    11941 aggaatcgtc cactcggaga tgtttccgct gctcgacaag agcaagccga acccggtcga
    12001 gttgtttcag atctggctca acctgccgcg cgcgggaaag atggtgaatc cgtacttctc
    12061 gatgctgtgg gatcacacga tcccgcgcca ggaagcgcgc gacgacgcgg gacgcaccac
    12121 cgagatcacg gtgatcgccg gcacggtcgg cgacgcgaag gcacctgccc cgccgcccga
    12181 ttcgtgggcc gcgcgcgccg atgccgacgt cgcgatctgg tcgatccgca tgatgcccgg
    12241 tgcgacgtgg acgctacctg cgacgcgtgc cagcacgaac cgcacgctct actttttccg
    12301 cggcacgtcc gtgcagatcg cgagtcgcaa gctcgagaag aacgccggcg tgcggctgcg
    12361 tcccgacgcc gacgtcacgg tggtcgccgg tcccgacggt gccgagctgc tgctcctgca
    12421 gggccgtccg atcaacgagc cggtcgtcca gtacggtccg ttcgtgatga acacgcccgc
    12481 ggagatccag caagcgttca tggactatcg ccgcacgcag ttcggcggct ggccgtggcc
    12541 gaaggatgat ccggtgcacg ggcgcgagga cgtgcgcttc gcgcgtcacg ccgacggcaa
    12601 gatcgagcgc gcggatggcg ggaccgccga tccgtccggt cactcgtcga tgaagccgct
    12661 cggctcgtcg accggcagcg tgtagcgctt cgtgatctcg ccgcggatca ccatgcggct
    12721 cgcttccgcc gacagcttcg tatcggtctc gacacgctcg actgcctcgg cgagctccgg
    12781 gtccttgacg ttgtcgcgga accacgtcga gtactcgcgc ttgcgcaggt gatatgtcca
    12841 ggtgtcgtcg tcgacacctt cggcgagcat cacgaacaac atcagatttg acgccttgag
    12901 attgagccgc ctgtcgggcc cgcggaagta gaagctgtgc gggccgaggt tcccctcgac
    12961 atacttgcgc gagtggcgcg tgcgctcggt gcggcactga tacgtctgga cgtcgaacgc
    13021 ctgcttgctg tcggtgtgcc agagcagcgt gtgacctgcc gccaactttt cggtgggcac
    13081 gtggggtgtc ttcttgacgg ccgcgacctt cgcgaactcc ttcacggtct tgtcgggatg
    13141 atcgccgacg acaagcagcg tattgatcga ttcgatcacc ggcttcgcga cgctgcccgg
    13201 atgcacggtg acgtagatcg tcccgcgcgg cctcagcgcg agctcggtgg cgggctccca
    13261 cgatcccggt agcaagtggt gggcttcgtc gacgacgagc cagtgcggcc ggccagtacg
    13321 cgtgcgtagc tcgacgagcg gcggcagtag ctgcgcgaag aactccgggc gatgctcgac
    13381 tgcgacgccg agcatgttga cgaccgcgtt gcggtgagcg tcgcgcaaca cgtccatgac
    13441 ctcgacgac
//
LOCUS       DASUFX010000833         5351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_554039,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000833 DASUFX010000000
VERSION     DASUFX010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554039"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFV99_33550"
     CDS             <1..562
                     /locus_tag="VFV99_33550"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HEX5064365.1"
                     /translation="KLGKDDLKGVHVAVQGVGHVGYYLCKELHAAGAKLTVADVDKLK
                     SERAQREFGAAVTSIDTVAAVECDVFAPCALGAGLNDNTIPHLRAKIVAGAANNQLAE
                     PRHGDDLYARGILYAPDYAINAGGLVNVAQEVKGYDAAASRAKTLEIYNTIWDICDRS
                     KKLQAPTYKVADIMVEEKLAAVGRKQ"
     gene            complement(563..775)
                     /locus_tag="VFV99_33555"
     CDS             complement(563..775)
                     /locus_tag="VFV99_33555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064366.1"
                     /translation="MPGTRSLADRLVDLREQANACRAQAIRLNAKLSLLERERDDLRG
                     MRERLAASSARLDHAIDDLMRRARRW"
     gene            complement(879..2678)
                     /locus_tag="VFV99_33560"
     CDS             complement(879..2678)
                     /locus_tag="VFV99_33560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064367.1"
                     /translation="MKRGLLIALVGCSGSVELPSPTPPPPWGAPITGGTMMVTRDGAR
                     AVVADPDRDRVMIVDLDSGKTTADIALEAGSDPGRVIEDGAGRAHIALRGTNSLLTLD
                     MATGEVRSQRLACTEPRGLAWDPATDLVHVACTSGELVSFPSGDGDAVRSIVLDRDLR
                     DVIVRGSQLAVTRFRTAEVLTLDAQGAVVARVTPPVVNRLNNGFLGDVPTPDGSGAGS
                     GSGSDATVPAPAEVAWRTIALPDGSMAMTHQRKVGQQLRLSPGGYGSTCGEQGLVESA
                     ITIVDPSGTPVAVAPFVHGALPVDVAVNPDATLLAFASAGSKSVQVVPMSAMGEHDDD
                     QCGDSDESRVMRYNDDLGAPTSVAYRANGELVVFYPEYPALVIHGTNMTSDRTVTLPG
                     TIGYDAGRALFHTQTAASIACASCHPEARDDGQVWDFDQLGTRRTQSLAGGILTRGPY
                     HWSGDMTDLQTLMSNVFSQRMFGGSLTHSQLVSLGPWLDRIPAPQSTTFAAADAVERG
                     QQLFMSQDLGCTTCHVGPQYTNNMLVNVGTGANLKVPSLLGVGVRAPFMHDGCAATLR
                     DRFGTCGGGDLHGKTAQLTAEQLDDLIAFLESL"
     gene            complement(2675..3691)
                     /locus_tag="VFV99_33565"
     CDS             complement(2675..3691)
                     /locus_tag="VFV99_33565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5064368.1"
                     /translation="MPVAIGTGMQFRELRRVLQAIVLAGLPACGLGEGVVGSLGGNGE
                     CERTVVKKFDFELPADPPLQLRVESCRVDADACLDLCAMLMTRAGLQAPSSCAVTFQF
                     GDTVHATASYTESTNNPSCGVEGRRPAGLVHPKRIDAHDAVGRWLAHAAWLEAASIPA
                     FIYLARELDMHGAPRGLARAALAAARDEIRHAKIMRQVAMRYGATVPSVDVALPAERS
                     LEELAIENAIEGCVRETWGAVVALWQAHRADDPELRAIYRGIADDEARHAALGWAIDQ
                     WVRTRLDAAAHARIDAAHEAAVREIFEGEHAEVLAVLGLPIGSEERGLLSRTYDTLWT
                     RRAA"
     gene            3821..5272
                     /locus_tag="VFV99_33570"
     CDS             3821..5272
                     /locus_tag="VFV99_33570"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5064369.1"
                     /translation="MNRIAVAFVTVGFVSAAHAGVEVGGTAGAHVFSETNAVGVPEGL
                     STHQANSAFFGFRLGVYFTKMLGVELEGGLIPTESAGAIATFDIYNVVGRGHLIAQFR
                     NENPDNKIIPFALVGGGLLSVVDISTPDDSQFKKESGPFGYVGVGAKYRATGGWGVRL
                     DGRLIGEKSTDDSITTDFELLFSLYKDFSAKSAPIKKEEPTKTAASDTDPDQDGIAGD
                     ADKCPKEAEDKDGFQDDDGCPDLDNDSDGIADAGDKCPAEPEDKDNFQDDDGCPDPDN
                     DADGVADATDKCADQPETKNGFEDDDGCPDELPAKLAAYTGTIAGINFKVNSADLLPA
                     SNKTLDKAVAVLAEFKDVKVEIQGHTDDQPLKAGGKFSDNDALSQARAEAVKAYFIKK
                     GVTEDRLVAKGFGGTVPVQDPAGLTGAKLNAARTVNRRVEFKLISPGGEEAKPEPKKE
                     EPKAEEPKPEEKKEEPKVEKAKAEKKAEEKKAE"
BASE COUNT          990 a         1788 c         1766 g          807 t
ORIGIN      
        1 caagctcggc aaggacgacc tcaagggcgt ccacgtcgcg gtccagggcg tcggccacgt
       61 cggctactac ctgtgcaagg agctgcatgc ggcgggcgcg aagctcaccg tcgccgatgt
      121 cgacaagctc aagagcgagc gagcgcagcg cgagttcggt gctgcagtga catcgatcga
      181 caccgtcgca gcggtcgaat gcgacgtgtt cgcgccgtgc gcgctcggcg ccggtctcaa
      241 cgacaacacg attccgcacc tgcgcgcgaa gatcgtggcc ggcgctgcga acaaccagct
      301 cgcggagccg cgccacggtg atgacctcta cgcccgcggc atcctctacg cgcccgacta
      361 cgcgatcaac gccggcggtc tcgtcaacgt cgcgcaagag gtgaagggct acgacgcggc
      421 cgcgtcgcgc gcgaagacgc tcgagatcta caacacgatc tgggacatct gcgatcgcag
      481 caagaagctg caagcgccga cgtacaaggt cgccgacatc atggtcgaag agaagctcgc
      541 ggcagtcggc cgcaagcagt agttaccagc ggcgcgcgcg gcgcatcagg tcgtcgatcg
      601 cgtgatcgag gcgcgccgag ctggccgcga gtcgctcgcg catgccgcgc aagtcgtcgc
      661 gttcgcgctc gagcagcgac agcttcgcgt tcagccgaat cgcctgtgcg cgacacgcat
      721 tcgcctgctc gcgcaggtca accaaccgat ccgcgagtga acgcgtgccc ggcataccta
      781 gtgtgatgac ccgccacgag ccgaggggtc acattttcgg cgctgacctt ccgtgacagc
      841 agctgactac agacctcgtg tagtcagaga tccgaccgtc acagcgactc gaggaacgcg
      901 atcagatcat cgagttgctc tgccgtgagc tgcgcagtct tgccgtggag atcaccaccg
      961 ccacacgtgc cgaatcggtc acggagcgtg gctgcgcaac catcgtgcat gaacggagcg
     1021 cgcacaccga cgccgagtag gctcggaact tttaagttag cgccggtacc aacattgacg
     1081 agcatgttgt tcgtgtactg cgggccgacg tggcaggtcg tgcaaccgag gtcttgcgac
     1141 atgaacagct gctggccgcg ctctaccgcg tcggcagctg cgaacgtcgt cgattgcgga
     1201 gccggaatgc gatcgagcca cggcccgagc gagacgagct ggctgtgggt gagcgagccg
     1261 ccgaacatgc gctggctgaa cacgttgctc atcagcgtct ggagatccgt catgtcgccg
     1321 gaccagtgat acggaccgcg cgtcaggatg ccgccggcga ggctttgcgt gcgacgcgtg
     1381 ccgagctgat cgaagtccca gacctggccg tcgtcgcgcg cctcgggatg acacgacgcg
     1441 cacgcgatac tcgcggccgt ctgcgtgtgg aacagcgcgc gacccgcgtc gtagccgatg
     1501 gtgcccggca gcgtcacggt gcgatcgctc gtcatgttcg tgccgtggat cacgagcgcc
     1561 ggatactcgg gatagaacac gacgagctcg ccattcgccc gatacgcaac cgacgtcggc
     1621 gcgccgaggt cgtcgttgta cctcatcacg cgtgactcgt cggagtcgcc acattgatcg
     1681 tcgtcgtgct cgcccatcgc ggacatcggc acgacctgca cgctcttgct gccggcgctc
     1741 gcgaacgcga gcaacgtcgc gtcgggattc accgcaacgt cgaccggcag cgcgccatga
     1801 acgaacggcg cgacggcaac gggcgtaccg ctcggatcga cgatcgtgat cgcgctctcg
     1861 acgaggcctt gctcgccgca cgtgctcccg tagccgcccg gtgagagccg gagctgctgg
     1921 ccaactttgc gttgatgcgt catcgccatg ctgccgtcgg gcagcgcgat cgtgcgccac
     1981 gcaacctcgg caggcgccgg caccgtcgcg tccgaacccg aaccagaacc tgcgcctgat
     2041 ccatcgggcg tcgggacgtc gccgaggaag ccattgttga ggcgattgac cacaggcggc
     2101 gtgacgcgcg cgacgaccgc gccctgcgca tcgagcgtga gcacctccgc ggtgcggaag
     2161 cgcgtgaccg cgagttgcga tccacgcacg atcacatcgc gcagatcgcg atcgagaacg
     2221 atcgagcgca cggcatcgcc gtcgccggag gggaagctca ccagctcgcc actcgtgcac
     2281 gcgacgtgaa cgaggtcggt cgccggatcc cacgcgaggc cgcgcggctc ggtgcatgcg
     2341 agccgttggc tgcgcacttc gccggtggcc atgtcgagcg tcagcagcga attggtgccg
     2401 cgcagcgcga tgtgcgcgcg accggcgccg tcttcgatca cgcgacccgg atcgctaccc
     2461 gcttcgagcg cgatgtccgc ggtcgtcttg ccgctatcca gatcgacgat catcacgcga
     2521 tcgcgatccg gatcggcgac gaccgcgcgc gcgccgtcac gcgtgaccat catcgtgccg
     2581 cccgtgatcg gcgcgcccca cggaggcggt ggcgtcggcg acggcagctc gacggagccc
     2641 gaacatccaa ccaatgcgat cagcaacccg cgcttcatgc tgctctcctg gtccaaagcg
     2701 tgtcgtacgt gcgtgaaagg agtccgcgtt cctcgctgcc gatcggcaga ccgagcaccg
     2761 cgaggacttc tgcgtgctca ccctcgaaga tctcgcgcac cgcagcctca tgtgccgcat
     2821 cgatgcgcgc gtgtgcggcc gcgtcgagac gcgtgcgcac ccattgatcg attgcccagc
     2881 cgagcgcggc gtggcgcgct tcgtcgtcgg cgatgccgcg atagatcgcg cgtagctcgg
     2941 gatcgtcggc acgatgcgcc tgccagagcg cgacaaccgc gccccaggtc tcgcgcacgc
     3001 agccctcgat cgcattctcg atcgcgagct cttcgagcga acgctccgcc ggcagtgcga
     3061 cgtcgacgct cggcacggtc gcgccgtaac gcatggcaac ctgccgcatg atcttcgcgt
     3121 gacgaatctc gtcgcgcgcc gcggccaatg cagcgcgcgc cagcccgcgt ggtgcgccat
     3181 gcatgtcgag ctcgcgcgcg agatagatga acgcgggaat cgatgccgcc tcgagccacg
     3241 cagcgtgcgc gagccagcga ccgaccgcgt cgtgcgcgtc gatgcgcttg ggatgcacga
     3301 gccccgcggg ccggcgacct tcaacgccgc agctcgggtt gttcgtcgat tccgtgtacg
     3361 acgccgtggc atgaacggtg tcgccgaact ggaacgtgac cgcgcagctg ctcggagcct
     3421 gcaacccggc ccgggtcatc agcatcgcgc acaggtcgag gcacgcatcg gcgtcgaccc
     3481 gacagctctc gacccggagt tgcaacggcg ggtcggccgg caactcgaag tcgaacttct
     3541 tgacgacggt gcgttcgcac tccccatttc cacccaggga gccgactacg ccctcgccaa
     3601 gaccgcaggc gggcaagccc gccaacacga tcgcttgcaa tacacgccgg agctcgcgga
     3661 actgcatgcc ggtgccgatt gcaacgggca ttccgccgtc acgacgcggt aaccgcgcga
     3721 atctcgcagc ctctgtttca gaaatctgag gcggcccggc tcgattttcg gctggttctg
     3781 cttgcgtcga accccgccat ggaaggacaa cccgaggccc atgaatcgaa ttgccgtagc
     3841 ctttgtcacg gtcggttttg tgtccgccgc tcacgcggga gtcgaggtcg gtggcactgc
     3901 gggcgctcac gtattcagcg aaacgaacgc ggtcggcgtg ccggagggcc tgtccaccca
     3961 ccaggccaac tcggcgttct tcggctttcg cctcggcgtc tacttcacga agatgctcgg
     4021 cgtcgagctc gagggtggcc tcatcccgac cgagtcggcg ggcgcgatcg ccacgttcga
     4081 catctacaac gtcgtgggtc gcggccatct gatcgctcag ttccgcaacg agaaccccga
     4141 caacaagatc atcccgttcg cgctcgtcgg cggcggtctc ctcagcgtgg tcgacatcag
     4201 cacgccggac gactcgcagt tcaagaagga gagcgggccg ttcggctatg tcggcgtcgg
     4261 cgccaagtac cgcgcaacgg gtggctgggg cgttcgtctc gatggacgcc tgatcggcga
     4321 gaagtcgacg gacgactcga tcacgaccga cttcgagctg ctcttttcgc tctacaagga
     4381 cttcagcgcg aagagcgcgc cgatcaagaa ggaagagccc accaagacgg cggccagcga
     4441 caccgatcca gatcaggacg gcatcgcggg tgacgcggac aagtgcccga aggaagccga
     4501 ggacaaggac ggcttccagg acgacgatgg ttgccccgac ctcgacaacg actccgacgg
     4561 catcgccgat gccggcgaca agtgcccggc cgagcccgag gacaaggaca acttccagga
     4621 cgacgacggc tgcccggatc cggacaacga cgcggacggc gttgccgacg cgaccgacaa
     4681 gtgtgccgat cagccggaga cgaagaacgg cttcgaggac gacgacggct gccccgacga
     4741 gctgcccgcg aagctcgccg catacaccgg cacgatcgct ggcatcaact tcaaggtgaa
     4801 ctcggccgac ctgttgccgg cgtcgaacaa gacgctcgac aaggcggtcg cggtgctcgc
     4861 cgagttcaag gacgtcaagg tcgagatcca gggccacacc gacgatcagc ccctcaaggc
     4921 gggcggcaag ttctccgaca acgacgcgct ctcgcaggcg cgtgcggaag cggtgaaggc
     4981 gtacttcatc aagaagggcg tcaccgagga tcgcctcgtc gcgaagggct tcggcggcac
     5041 ggttccggtt caggatccgg cgggcctcac gggcgccaag ctcaacgcag cgcgcacggt
     5101 gaaccgccgc gtcgagttca agttgatcag ccccggcggc gaggaagcga agccggagcc
     5161 gaagaaggaa gagccgaagg cggaggagcc gaagcccgaa gagaagaagg aagagccgaa
     5221 ggtcgagaag gcgaaggcgg agaagaaggc ggaagagaag aaggcggagt aactctcgcg
     5281 tcgtctcttc gaacgcggat cgcgcgcaac gcggtccgcg tttttcgttt cggcgcaacg
     5341 ggataaggtc g
//
LOCUS       DASUFX010000834         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_691942,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000834 DASUFX010000000
VERSION     DASUFX010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691942"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFV99_33575"
     CDS             complement(<1..381)
                     /locus_tag="VFV99_33575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064370.1"
                     /translation="MRDLVITARPDGSWFTWVVEVVNKSRDSVSVDWDQSSLVAGNGD
                     SMGRLLRGETRRIDSANVQPKTPIPPGARVSQWVIPEQMTMMSGASEGPLSPMPPLAY
                     PGARLVISVERKGKTASWTGVLTAT"
     gene            complement(663..>3452)
                     /locus_tag="VFV99_33580"
     CDS             complement(663..>3452)
                     /locus_tag="VFV99_33580"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5064371.1"
                     /translation="VSQQQPSEPGLDAGMRIHQYELIRELGRGGMGVVWAARDTKLGR
                     RVAIKFLLDANREVAERFLTEARATAQCNHDNIVIIHEVDELDGMPYMVLEFLEGQQM
                     RDLMGTYGAGIRMPASRVVELMLPVARALERAHELGIVHRDLKPENVFVTNAGTIKVL
                     DFGIAKAVSKREAAAAQPQRAQSHYGVEPISMNLTRDGTLVGTLPYMSPEQMGVGEVD
                     HRADIWSCGIMMFELLAGRHPIYPLTTEALFENAVTDALMPPVRGEVSDVPDALASIV
                     DGCLRKRIPERIPTAKELVRRLEQLMPGRAGRQLAEGESPYPGLTAFQESDADRFFGR
                     SRDIARMTARVREYPITGIVGPSGVGKSSFIRAGVGPALKASGERWDVVTLRPGRAPL
                     AALASIVQKLTTRSGPDVQTQVNEHHQIVHRLRTEPGYVGTLLRTRARQTNGQILLFI
                     DQFEELYTLVPDADERKAFTAALAGVADDTAAPLRVVVSMRSDFLDRIAEDPRFMEEM
                     SRGLVFLQPPDRNGLREALVAPIEMVGYRFESATMIEDMLDALAGASGALPLLQFAAG
                     KLWDARDRERRLLTVQSYNAIGGISGALATHADDVVAQMNDRQQKLTQKIFRRLVTPE
                     RTRAIVELGDLYQLAGDQHEVGRTIDSLVQARLLVVQTRGDAGGGSVEIVHESLIDRW
                     PTLRRWLDEDQEDAAFLSQLQAAAKQWEAKRHAAGLLWRGDAMEEARRWHNQRPRELP
                     PREQAFLSAVFALARRGKRVKRAALLAVFIVLGGAAAAASVGMLMIRSAEQEASENAK
                     KATENAARAEHEATIAKQALEEREKKEQERLAAEEKQKAAEADKAKVEAEKVQVEAQK
                     AQAETKVAESAEELKKKNKELEGKIAEVEKAKAKAETATKEAQTAKADVDRINKKLEA
                     EIKELKKGGLVEKLK"
BASE COUNT          499 a         1141 c         1163 g          649 t
ORIGIN      
        1 cgtcgccgtg aggacgccag tccaggatgc tgtctttcct tttcgctcga cggagatcac
       61 gaggcgcgcg ccgggataag cgagcggtgg cataggcgag agtggacctt cgctggcacc
      121 cgacatcatt gtcatttgct cgggaatgac ccattgagag acgcgcgcac ccggaggaat
      181 cggcgtcttc ggctgcacgt tggcgctatc gatcctccgt gtctcgcctc tcagcaacct
      241 gcccatcgag tcgccatttc ccgccacgag gctcgattga tcccagtcga ccgagacgct
      301 gtcacgcgac ttgttcacga cctcgacgac ccaagtgaac cagctgccat cgggccgcgc
      361 cgtgatcacg agatcgcgca cagcgcgatc ctcccgcatt gtataaaatg gctgaacggt
      421 ttctgcgtac gtcggtacag cttgtcgagc gtgtcgttcc tcgggttcac cggcgagcgg
      481 atcgggaggc gggctctcgg caggcggcct ctcgttcgtc gtctcaacgg gggcgttcac
      541 tcttggctgc agaggcggcg gcccgctcga gcacgcgagc aacaaggcga tgaccggcac
      601 gctcttcatc attcgccgat cgtctcctct cggcgtcgca aacacaacct gacgcgtcgc
      661 cgctacttca gcttctcgac caagccaccc tttttgagct ccttgatctc ggcttcgagc
      721 ttcttgttga tgcgatcgac gtcggccttc gcggtctgcg cttccttggt cgcggtttcg
      781 gcctttgcct ttgccttctc gacctcggcg atcttgccct cgagctcctt gttcttcttc
      841 ttgagctctt cggcggactc ggcgaccttg gtttcggcct gagccttctg ggcctcgacc
      901 tgaacctttt cggcctcgac cttcgctttg tcggcttcgg cggccttctg cttctcttcg
      961 gcagcgaggc gttcttgttc cttcttctcg cgttcttcga gcgcttgctt cgcgatcgtc
     1021 gcttcgtgct cggcacgcgc ggcgttctcg gtcgccttct tcgcgttctc gctcgcttcc
     1081 tgctcggcgg agcggatcat cagcatgccg acgctcgcgg ccgcggcggc accgccgagc
     1141 acgatgaaga cggcgagcag tgcggcgcgc ttgacccgct tgccacgacg ggcgagcgcg
     1201 aacacggcgc tcaggaatgc ttgctcgcgc ggcggcagct cgcgcggacg ttggttgtgc
     1261 cagcggcgcg cttcctccat cgcatcaccg cgccagagca agccggcggc gtggcgcttc
     1321 gcttcccact gcttcgcggc ggcttgcagc tgcgacagga acgcggcgtc ttcctgatct
     1381 tcgtcgagcc agcggcgtag cgtcggccag cgatcgatca gcgactcgtg aacgatctct
     1441 accgagccac cgcccgcgtc accgcgcgtc tgcacgacga gcaggcgggc ctggacgagc
     1501 gagtcgatcg tccggccgac ctcgtgctga tcgccggcga gctgatagag gtcgccgagc
     1561 tcgacgatcg cgcgcgttcg ctcgggcgtg acgagtcggc ggaagatctt ctgcgtcagc
     1621 ttctgctgcc gatcgttcat ctgcgcgacg acgtcgtcgg cgtgcgtcgc gagcgcaccg
     1681 ctgatgccgc cgatcgcgtt gtagctctgc acggtgagga ggcggcgctc gcggtcgcgc
     1741 gcgtcccaca gcttgccggc ggcgaactgg agcaggggca gggcacccga cgcaccggcg
     1801 agcgcgtcga gcatgtcctc gatcatcgtg gcgctctcga agcggtagcc gaccatctcg
     1861 atcggtgcga cgagtgcctc gcggagcccg ttgcgatccg gcggctggag gaacacgagg
     1921 ccgcgcgaca tctcctccat gaagcgcgga tcctcggcga tgcgatcgag gaagtcggag
     1981 cgcatcgaca cgacgacgcg cagtggcgcg gcggtgtcgt cggcgacgcc cgcgagtgcg
     2041 gcggtgaatg ctttgcgctc gtcggcgtcg gggacgagcg tgtagagctc ttcgaactga
     2101 tcgatgaaca gtaggatctg gccgttcgtc tggcgcgccc gcgtgcgcag cagcgtgccg
     2161 acgtaaccgg gctcggtgcg cagccgatgg acgatctggt ggtgctcgtt gacctgcgtc
     2221 tgcacgtcgg ggcccgagcg cgtggtcagc ttctgcacga tgctcgcgag cgcggcgagc
     2281 ggggcgcggc ccgggcgcag cgtgacgacg tcccagcgct cgcccgacgc cttgagcgcg
     2341 gggccgacac cggcgcgtat gaacgacgac ttgccgacac ccgacgggcc gacgatgccg
     2401 gtgatcggat actcgcgaac gcgcgcggtc atgcgcgcga tgtcgcgcga gcgaccgaag
     2461 aagcgatcgg catcgctctc ctggaatgcg gtgaggccag ggtaggggct ctcgccctcg
     2521 gcgagctggc gaccggcgcg gccgggcatc agctgctcga gccggcgcac cagctccttc
     2581 gcggtcggga tgcgctcggg gatgcgcttg cggaggcagc cgtcgacgat gctcgcgagc
     2641 gcgtcgggaa cgtcggaaac ttcgccgcgc accggcggca tcagggcgtc ggtgaccgcg
     2701 ttctcgaaca gcgcctcggt cgtcagcgga tagatcggat ggcggcccgc gagcagctcg
     2761 aacatcatga tgccgcacga ccagatatcg gcgcggtgat cgacctcgcc gacgcccatc
     2821 tgctcgggcg acatgtacgg tagcgtgccg acgagcgtgc cgtcacgcgt gaggttcatg
     2881 ctgatcggtt cgacgccgta atgcgattgc gcacgttgcg gctgcgcggc cgcggcctcg
     2941 cgcttggaga ccgccttcgc gatgccgaag tcgagcacct tgatcgtgcc cgcgttcgtg
     3001 acgaacacgt tctcgggctt gagatcgcga tgcacgatgc cgagctcgtg tgcgcgttcc
     3061 aacgcgcgtg cgaccggcag catgagctcg acgacgcgcg acgcgggcat gcgaatgcca
     3121 gcgccatacg tgcccatcag gtcgcgcatc tgctgaccct cgaggaactc gagcaccatg
     3181 tacggcatgc cgtcgagctc gtcgacttcg tggatgatga cgatgttgtc gtggttgcac
     3241 tgcgcggtgg cgcgcgcctc ggtgaggaag cgttcggcga cttcgcgatt ggcgtcgagc
     3301 aagaacttga tcgcgacgcg gcgaccgagc ttggtgtcac gcgcggccca cacgacaccc
     3361 atgccgccgc gaccgagctc gcggatcagc tcgtactgat ggatgcgcat gccggcgtcg
     3421 agaccgggct cgctcggttg ctgttgcgag ac
//
LOCUS       DASUFX010000835         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_134931,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000835 DASUFX010000000
VERSION     DASUFX010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134931"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFV99_33585"
     CDS             complement(<1..929)
                     /locus_tag="VFV99_33585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064372.1"
                     /translation="MADGAVSAAAKLPPRIGRHAVLGYLADGGMAEIFLGKESDGRPV
                     VIKRILPHLARQQNFVSMFIDEARIGSLVKHPNVVEIYELGQVGTDLFMVMEYLAGES
                     VSGVLRRHELRGDRLDVALGAYVIAEACAGLHAAHELRDADGNPAGVIHRDISPSNLF
                     ITYGGEVKVLDFGIATAHDRLSRTATGHVKGKFSYMSPEQCLGIPMDAKSDLWSLGVV
                     LYELTTQRRLFKRPNELLVLKAVTQDPIPRPRKEQLQYPQLLEQIVMRALQREPSRRP
                     HSALELREQLLDAMAKLGFTGDPRTELARVMTTL"
     gene            873..1787
                     /locus_tag="VFV99_33590"
     CDS             873..1787
                     /locus_tag="VFV99_33590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064373.1"
                     /translation="MSANARRELRRRTDRTIRHGRVIIAMTRAAFVGFVLLVSTAALA
                     VADNSGIVDPFAKQQPSHSDSASRKRAAELSAQSAQHYKRGEFEIAAALLKQAYALYA
                     EPNLLYNLARSLEGMGDKAGAVDAYTRYLDAGKQIEDRGAIERRIATLKADLAVQQRA
                     QHPESDSQPTQQPQRDVSRPAQQVPAARDEEVQPVDRVTPPSKLPWLPIVGGIAVIGG
                     GVGFGVAAKNLEQQATDEPSALDAKDLHDSARRDAMIANVMFAVGGAALVSGIVWEVF
                     VLRAQSKKTDVPRPVVTTRGLALEWTLP"
     gene            1784..2533
                     /locus_tag="VFV99_33595"
     CDS             1784..2533
                     /locus_tag="VFV99_33595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064374.1"
                     /translation="MIRIGFRLWLVLALAACSVKELDYTGKSCPCPSGYVCNEPEQTC
                     VRSLALDGGDPKDGDGSGSDATATDSCIPNARANLAYATGFSDFLNAWLPSAGSWSYQ
                     SGAELQQQSDVATLAWAYYMMNGGQSYRVVASMHMLDINGGSLGIGFHIGNTGNMYTC
                     TFAPTSGDLSLILTQNLGDIVLQATTVPFGDPRQPFTMEIQTVGAAHSCCLRTVMGSL
                     ITATDSALMSGNPGVVTREAAGGFTTFAVYQ"
     gene            complement(2537..3175)
                     /locus_tag="VFV99_33600"
     CDS             complement(2537..3175)
                     /locus_tag="VFV99_33600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064375.1"
                     /translation="MGANIITKTDRQMEDRLLAVSGDPLRADTLSKARAFKRTWLELA
                     EALTKVQAKRSWEEWGFTDFDAYCRKELHLRGSTVAKLLGSFRFLETSAPRVIERARA
                     DGFEAPIPSLPAVEFVQKATERAAADPETIETIKRVAFDEGLDAPLLAKKFKDVAFPE
                     TDRDKREKLRAGIAGTARKLSSMIAEDGSPVPKKLAIRVEEIVGELLEAIEN"
     gene            3286..4035
                     /locus_tag="VFV99_33605"
     CDS             3286..4035
                     /locus_tag="VFV99_33605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064376.1"
                     /translation="MRSFFVAALVLLAGCPGSAEDNALGGGDDQGSGSGSGGGGAPFN
                     ECHVAADCVPAGPKCCDCPTHAVPASDPVQQACKNVDCPPKDCGSPMEAACEQGRCVL
                     ACSPVPCDATVSCTDGFDTDANGCLVCACAAPTFTECAVDGDCSRVKADCCGCEMGGN
                     DAAIPTSQVASHDAMLMCPQNPSCPQVNTCAPDLSARCIAGTCTLVSGALPANACGRP
                     DLPACAQDEACTVNANDQATMQGVGVCQPPP"
     gene            4080..>4716
                     /locus_tag="VFV99_33610"
     CDS             4080..>4716
                     /locus_tag="VFV99_33610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064377.1"
                     /translation="MSFDPPASGSFPQAPPAPSLVGEVIDGRYRIFKKLGEGGMGEVY
                     AAEHVHIDKKFALKLLKAEIVSNSEAVKRFQQEARSSSSIKHRNIIAVEDFGRLPDGR
                     IYMCMELLGGAPLNDMIQQPMAADRLLNILIQAGHGLAAAHAKGIVHRDMKPENIYVT
                     QDASGDVPKLLDFGIAKVAGNDGQNNLTRTGTIFGTPFYMAPEQALGQQVDG"
BASE COUNT          821 a         1527 c         1547 g          821 t
ORIGIN      
        1 agcgtcgtca tcacgcgcgc gagctcggtg cgtggatccc cggtgaaccc gagcttcgcc
       61 atcgcatcga gcaattgctc gcgcagctcg agtgccgaat gcggacgccg cgatggctcg
      121 cgctgtagtg cgcgcatcac gatctgttcg agcagctgcg gatattgcag ctgttctttg
      181 cgtgggcgcg gaatcggatc ctgggtgacc gccttgagca cgagcagctc gttcggccgc
      241 ttgaacagcc ggcgctgcgt cgtcagctcg tagagcacga cgccgagcga ccacagatcg
      301 ctctttgcat ccatcggaat gccgaggcac tgctccggcg acatatacga gaatttgcct
      361 ttgacgtgac cggtcgcggt gcgcgacagc cggtcgtgcg cggtcgcgat tccgaagtcg
      421 aggactttga cctcaccgcc gtacgtgatg aacaagttcg acggcgagat gtcgcgatgg
      481 atcacgccgg cagggttgcc gtcggcgtcg cggagctcgt gcgcggcatg caggcccgcg
      541 cacgcttcgg cgatcacgta cgcaccgagc gcgacgtcga gacgatcgcc gcgtagctcg
      601 tggcgccgga gcacccccga gacactctcg cccgcgaggt actccatcac catgaacaga
      661 tcggtgccga cctggccgag ctcgtaaatc tcgacgacgt tgggatgttt gacgagcgag
      721 ccgatgcgcg cttcgtcgat gaacatcgac acgaagttct gctggcgcgc taggtgcggc
      781 aggatgcgct tgatgacgac cgggcggccg tcggactcct tgccgagaaa aatctccgcc
      841 atcccgccat ctgcgaggta gccgagcacg gcgtgtcggc caatgcgcgg cgggagcttc
      901 gccgccgcac tgaccgcacc atccgccatg gtagggtgat tatagccatg acgcgcgctg
      961 ccttcgtcgg cttcgtgctg ctggtctcga cggcggcgct ggcggttgct gacaacagcg
     1021 gcatcgtcga tccgttcgca aagcagcagc cgtcgcatag cgattctgcg agccgcaagc
     1081 gtgcggccga gctctcggcg cagagcgcgc aacattacaa gcgcggtgaa ttcgagatcg
     1141 cggcagcgtt attgaagcag gcgtacgcgc tctatgccga gccgaacttg ctctacaacc
     1201 tcgcgcgctc gctcgagggc atgggcgaca aggccggcgc ggttgatgcg tacacgcgct
     1261 atctcgacgc tggcaagcag atcgaagatc gcggtgcgat cgaacgccga atcgcgacgc
     1321 tgaaggccga tctcgcggtg cagcagcgcg cgcaacaccc agagtcggac tcgcaaccca
     1381 cgcaacaacc gcagcgcgat gtgtcgcgtc cggcgcaaca ggtgcccgcg gcgcgcgatg
     1441 aagaggtcca gccggtcgac cgcgtgacac cgccgagcaa attaccgtgg cttccgatcg
     1501 taggcggcat tgctgtgatc ggcggaggtg tcgggtttgg cgttgcggcg aagaatctcg
     1561 aacagcaagc gaccgacgag ccgagcgcgc tcgatgcaaa agatcttcac gacagcgcgc
     1621 gccgtgatgc gatgatcgcg aacgtgatgt ttgcggtcgg cggcgcggca ctcgtgagcg
     1681 gcatcgtgtg ggaggtgttc gtgctgcgcg cgcagagcaa gaagacggat gtgccgcgtc
     1741 cggtcgtgac gacgcgtggc ctcgcgctgg agtggacgct gccgtgatcc ggatcggttt
     1801 ccgcctctgg ctggtcctcg cgctcgcggc gtgctcggtc aaggagctcg actacaccgg
     1861 caagagctgt ccgtgtccgt cgggctacgt ctgcaacgag cccgaacaaa cgtgcgtacg
     1921 ctcgctcgcg ctcgacggtg gcgacccgaa agacggcgac ggcagcggca gcgacgcgac
     1981 ggctaccgac agttgtattc cgaacgcacg cgcgaacctc gcgtacgcga ccgggttcag
     2041 cgacttcttg aacgcgtggc ttccgtcggc cggttcgtgg tcgtaccaga gcggcgccga
     2101 gctccagcag caaagcgacg tcgcgacgct cgcgtgggcc tattacatga tgaacggtgg
     2161 ccagagctac cgcgtcgtcg cgagcatgca catgctcgac atcaacggcg gcagcctcgg
     2221 cattggcttc cacattggca acacgggcaa catgtacacg tgcacgtttg ctccgacgag
     2281 cggcgatctg tcgctgatcc tgacgcagaa cctcggtgac atcgttctgc aggcaacgac
     2341 tgttccattc ggcgatccgc ggcagccgtt cacgatggag atccagacgg tcggcgcggc
     2401 gcactcgtgc tgcctccgca ctgtgatggg ctcgctgatc accgcgaccg acagcgccct
     2461 catgagcggt aatcccggcg tggtgacgcg cgaggccgcc ggcgggttta cgacgttcgc
     2521 cgtctaccag tagcggtcag ttctcgatcg cttcgagcaa ctcgccgacg atctcttcga
     2581 cgcgaattgc gagcttcttc ggcaccggcg aaccgtcctc cgcgatcatc gacgacagct
     2641 tgcgcgcggt gcccgcgata ccggcgcgca gcttctcgcg cttgtcgcga tcggtctcgg
     2701 ggaacgcgac gtccttgaac ttcttcgcga gcagcggggc gtcgagaccc tcgtcgaacg
     2761 cgacgcgttt gatggtctcg atcgtctccg gatcagcggc cgcgcgctcg gtcgccttct
     2821 ggacgaactc gaccgcaggc agcgacggaa tcggcgcttc gaagccgtcg gcgcgggcgc
     2881 gctcgatcac gcgcggcgcg ctggtctcga ggaagcggaa gctgccgagc agcttggcga
     2941 cggtcgagcc gcgcaagtga agctccttgc ggcagtacgc gtcgaagtcg gtgaagcccc
     3001 actcctccca cgaccgcttg gcctggactt tcgtcagcgc ctccgcgagc tcgagccagg
     3061 tgcgcttgaa cgcgcgggcc ttcgaaagcg tatccgcgcg gagcgggtcc cccgacaccg
     3121 caagcaagcg atcttccatc tgccggtcgg tcttcgtgat gatgttggcg cccatcgctg
     3181 cagcacccta aaagaagggt ctgatcgcct cgaaaaactg agagcagacc tcgcgatctc
     3241 gattggttag actgggcacg tctcctgctg atacccatac ggtccatgcg cagcttcttc
     3301 gtcgccgccc ttgtcctgct ggccggttgt cccggctcgg cggaggacaa tgcgcttggc
     3361 ggcggcgatg accagggcag tggctcgggc agcggcggcg gtggcgcgcc gttcaacgag
     3421 tgccacgtcg cagccgattg cgtcccggcg gggcccaagt gctgcgattg cccgacgcac
     3481 gccgtgccgg ccagcgatcc ggtgcagcag gcctgcaaga acgtcgactg tccgccgaag
     3541 gattgcggca gcccgatgga ggccgcctgc gaacagggta ggtgcgtgct ggcgtgctcg
     3601 ccggttccgt gtgacgcgac cgtcagctgc accgacggct tcgacaccga cgccaacggc
     3661 tgcctcgtct gcgcctgtgc ggcaccgacg ttcaccgagt gcgccgtcga cggcgattgc
     3721 tcgcgggtca aggcagattg ctgtggttgc gaaatgggtg gcaacgacgc cgcgatcccg
     3781 acgagccagg tcgcctccca cgacgcgatg ctcatgtgtc ctcagaatcc ttcgtgtccg
     3841 caggtcaaca cctgcgcgcc cgacctctcg gcgcgctgca tcgcaggtac gtgcacgctg
     3901 gtatctggcg cgttgccggc caatgcctgc ggccggccgg atctaccggc gtgcgcgcaa
     3961 gatgaagcgt gtacggttaa cgcgaacgat caggcgacga tgcaaggtgt cggggtgtgt
     4021 caaccgccac cctgaggtca gaccattctc ccccgtcggc acgttataat cggggcctaa
     4081 tgagttttga ccctcctgca tcggggtcgt ttccgcaggc acctcccgca ccttcgctgg
     4141 tgggcgaggt gatcgacggg cgctaccgga ttttcaagaa gcttggcgaa ggtggcatgg
     4201 gcgaggtcta cgcggccgag cacgtgcaca tcgacaagaa gttcgcgctc aagctcctca
     4261 aagcggaaat cgtttcgaac tcggaagcgg tcaagcgctt ccagcaagag gcgcgctcgt
     4321 cgtcatcgat caagcatcgc aacatcatcg cggtcgagga cttcggccgc ctgcctgacg
     4381 gccgcatcta catgtgcatg gagctcctcg gtggcgctcc gctcaacgac atgatccagc
     4441 agccgatggc tgccgatcgg ctgctcaaca tcctgatcca agccggtcac ggtctcgctg
     4501 ctgcgcacgc gaagggcatc gtccatcgcg acatgaagcc ggagaacatc tacgtcacgc
     4561 aggatgcaag cggcgacgta cccaagctcc tcgacttcgg tatcgcgaag gttgccggca
     4621 acgacggcca gaacaacctg actcgcaccg gcacgatctt cggcacgccg ttctacatgg
     4681 cgcccgagca ggcgctcggc cagcaggtcg acggcc
//
LOCUS       DASUFX010000836         5741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1247625, whole genome shotgun sequence.
ACCESSION   DASUFX010000836 DASUFX010000000
VERSION     DASUFX010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247625"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFV99_33615"
     CDS             <1..408
                     /locus_tag="VFV99_33615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064378.1"
                     /translation="CDRGGMRWIAWLCLVTSGCSFLFIPRVDPQRPADCNPSRKAVAG
                     DVGAALGELTPFAIFLLFAVDHQTCINGNCHHVEGDPDFVAPATVAGLLITTHLVSAV
                     VGVTRTRECRDLRGRMAPPQPPPYGGPPGAMSY"
     gene            complement(402..1796)
                     /gene="murD"
                     /locus_tag="VFV99_33620"
     CDS             complement(402..1796)
                     /gene="murD"
                     /locus_tag="VFV99_33620"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5064379.1"
                     /translation="MNLEGKKVVVVGLALTGVAVAKFCVRRGAHVIVTDGKPAEKLAD
                     QMKLLDGVPVTWQLGGHDLATFTSADLVVMSPGVPTLPEMTAARKAGVEVIAEIELAY
                     RFLDPGATLIAITGTNGKSTTTALTGKLCEHSGRPTFCGGNLGNMPLIDAVDHPANVP
                     GGWIVAEVAAFMLENCTSFRAHAGVLTNVTEDHLDRFGTMERYAEMKGRIWDWQTPGD
                     VAIANAADEWTMRETTGIKSQLYTFDSRKGAALSGTRGAFLDGKNIVLRLPDEERYPM
                     DDLVIVGNHNMENAMCAYLATRTLGLPPDVVRAGARAYRPLPHRMELVGDKANIYYYD
                     DSKGTNVASVAASVRGFPRPLVLIAGGVDKGGSYEPMLDALDGVCKGIILIGKAAPLI
                     RDAAATHKVQYPVLDANDMHDAVRRATELCGSGDAVVLSPACASYDMFQNFGHRGRVF
                     REAVSAVGAKRLDQ"
     gene            complement(1800..2960)
                     /gene="mraY"
                     /locus_tag="VFV99_33625"
     CDS             complement(1800..2960)
                     /gene="mraY"
                     /locus_tag="VFV99_33625"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5064380.1"
                     /translation="MLFHLFYGAFADKIGWLRVFRYPSTRILCAAITALLLSFLIGPW
                     FIERLKSRNVGEKIRDDGPATHKKKAGTPTMGGSLILFCLAVSTLLWCDLRSQLVWLA
                     ISVTIAFGAIGFADDYAKLTKRKDGLSGKLRLLLEFLFAGAAMAWLFYSNVMHTDVRL
                     HVQLPFTNFYEQGLVIPAWAYAVFGAFVVVGTANAVNLTDGLDGLAIGPSIMNAGVFL
                     ILAYISGVPTTILSSQGPVTIAQYLHVAHIPGAEELAVFAAALFGSGIGFLWYNTYPA
                     QVFMGDVGALGLGGAIGMLAVLTKNELVLLIVGGLFVVEALSAMIQTGVYKATKRRDP
                     TTGKMVGKRVFLKAPIHHHYEELGWDEPKIIVRFWLVSALLALVALGTLKMR"
     gene            complement(2961..>5741)
                     /locus_tag="VFV99_33630"
     CDS             complement(2961..>5741)
                     /locus_tag="VFV99_33630"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5064381.1"
                     /translation="DSRTVQPGDVFVAIKGMRSDGHAFVPSVVQQGAIAVVVERELDV
                     KVPQIVVPSTSRALGPLVARSLGNPAAGMTLIGITGTNGKTTTTYLVESILLAAGHKP
                     GVIGTVEVRWPGGSESASYTTPTPQLLHGAFAKMREAGCTHVVMEVTSSALALDRVVG
                     LTFAVGGFSNLTQDHLDVHGTMEAYRAAKHRLFAEHLKGGTAVVNVDDPEGEGMAGAA
                     DPRHTSTLRVSVEGRLSDIRVTFQESTVKGIHASIATPTGAVPIEAKPLIGQYNVANL
                     ALAVGIAEALKIPHDAIAAGIAELKGVPGRVERVVNGADLDILVDYAHTPDALRNVLT
                     ALRPLTRRRLICVFGCGGDRDPTKRPKMGAAVAELADLAVVTSDNPRTEDPRKILDQI
                     LPAVPKPFFVDVDRRAAIRAAIAEATPGDVVVIAGKGHEDYQILGTTKIHFDDREEAA
                     AAAAEREQRLVAGLARDAGGELLGDGNVNCDRVVIDSRVVAPGALYVAIRGEQHDGHR
                     FCLDAVKAGATAVMVDKEGKRWAPAAAPDLLAPELRSLRNAAIVVDDTRLALGKIARS
                     HRRAWASDTRKLVAITGSAGKTTTKELTRCALAMAGTTHAAEGSLNNETGLPLTLLGL
                     RLFHQYGVVEMGMRGLGQIEYLTKIAEPDVAVVVNAGSAHIELLGSTDAIAQAKSEIW
                     LGLRPGGTVVLPAGDQRLEYWARQHQPTARHVTFGDGDADVRLIEYKPTDAGAICQLD
                     VFGDKHELKLQLVGKHAAIDACAALAAAHAAGASSAQALAGLSRALPVSMRGEVIELG
                     GRKVIVDCYNANPASMTAALHSLAERAHGLPAIAVLGDMLELGDHAPAAHKEIGELAN
                     KLGIGVVGIGEMAKYIVDAAGKDAELSPSHAAAAERALARTESAGGWILLKASRGMKL
                     ERVLEVMKEQTP"
BASE COUNT          929 a         1880 c         1935 g          997 t
ORIGIN      
        1 tgcgatcgtg gaggaatgcg gtggatcgcg tggctctgtc ttgtcacgtc cggatgttcg
       61 ttcttgttca ttccgcgggt cgatccgcag cggcccgccg actgcaatcc gtcgcgcaaa
      121 gcggttgctg gcgacgtcgg cgccgcgctc ggcgagctca cgccgtttgc gatcttcttg
      181 ttgttcgcgg tcgatcatca gacgtgtatc aacggcaact gccaccacgt cgaaggagat
      241 ccggacttcg tcgcgccggc gacggttgcc gggctcttga tcacgacgca cctcgtgtcg
      301 gccgtcgtcg gtgtgacacg cacgcgcgag tgccgcgatc tgcgcggacg aatggcacca
      361 ccgcagccgc caccgtacgg cgggccaccg ggtgcaatgt cctattgatc gagtcgcttc
      421 gcgccgaccg cactcaccgc ttcgcggaac acgcgaccgc ggtggccaaa gttctggaac
      481 atgtcgtagc tcgcacacgc gggcgacagc acgacggcat cgccgctgcc gcagagctcg
      541 gtcgcgcggc gcaccgcatc gtgcatgtcg ttcgcgtcga gcaccgggta ttgcaccttg
      601 tgcgtggcgg cagcgtcgcg gatcagtggc gccgctttgc cgatcaagat gatgcctttg
      661 cagacgccat cgagcgcgtc gagcatcggc tcgtacgaac cgcccttgtc gacgccgccg
      721 gcaatcagca cgagtgggcg cggaaagccg cgcaccgatg ccgcgaccga cgcgacgttc
      781 gtgcctttgc tgtcgtcgta gtagtagatg ttcgctttgt cgccgacgag ctccatgcgg
      841 tgcggcaacg ggcgataagc gcgggcacct gcgcggacga cgtcgggcgg caacccgagc
      901 gtgcgcgtcg cgaggtacgc gcacatcgcg ttttccatgt tgtggttacc gacgatcacg
      961 agatcgtcca tcgggtagcg ttcttcgtcc ggaaggcgga gcacgatgtt cttgccgtcg
     1021 aggaatgcgc cacgggttcc ggacagtgcc gcgcccttgc gcgaatcgaa cgtgtagagc
     1081 tggctcttga tgcccgttgt ctcgcgcatc gtccactcgt cggctgcgtt cgcgatcgcg
     1141 acgtcgccgg gcgtctgcca gtcccagatc cgccccttca tctcggcgta gcgttccatc
     1201 gtgccgaagc gatcgagatg atcctcggtg acgttcgtga gcacgccggc atgcgcgcgg
     1261 aacgacgtgc agttctcgag catgaacgcc gcgacctcgg cgacgatcca gccgccgggc
     1321 acgttcgccg ggtgatcgac cgcgtcgatc aacggcatgt tgccgagatt gccgccgcag
     1381 aacgtcggcc gaccgctgtg ctcgcagagc ttgccggtga gcgctgtcgt cgtcgacttg
     1441 ccgttcgtgc cggtgatcgc gatcagcgtc gcgccaggat cgaggaaacg atacgcgagc
     1501 tcgatttcgg cgatcacttc gacgcctgcc ttgcgcgcgg ccgtcatctc gggaagcgtc
     1561 ggcacgccgg gggacatgac gacgagatcc gcggatgtga acgtcgcgag atcgtggccg
     1621 ccgagctgcc aggtaaccgg cacgccgtcg agcagcttca tctgatcggc gagtttctcg
     1681 gcgggtttgc cgtcggtgac gatgacgtgc gcgccgcgtc gcacgcagaa cttggcgacc
     1741 gcgacgccgg tcagcgcgag accgacgacg acgaccttct taccttcgag gttcatacgt
     1801 cacctcatct tcagcgtgcc gagcgcgacg agcgcgagca gcgccgatac cagccagaac
     1861 cgcacgatga tcttcggctc gtcccagccg agctcttcgt agtgatgatg gatcggtgct
     1921 ttcaggaata cgcgcttgcc gaccatcttg ccggtggtgg ggtcacggcg ctttgtcgct
     1981 ttgtagactc cggtctggat catcgcggac agcgcctcga cgacgaacag gccgcccacg
     2041 atcaggagca cgagctcgtt cttcgtcagc accgcaagca tgccgatcgc accgccgagg
     2101 ccgagcgcac cgacatcgcc catgaatacc tgcgcgggat aggtgttgta ccagaggaag
     2161 ccgatgccgc tgccaaacag cgcggccgcg aacaccgcga gctcttcggc gccagggatg
     2221 tgcgcgacgt gaaggtactg cgcgatcgtc accggtccct gagagctgag gatcgtcgtc
     2281 gggacgccgc tgatgtacgc gaggatgagg aacacgcccg cgttcatgat cgatggaccg
     2341 atcgcgagac cgtcgagacc gtcggtcagg ttcactgcgt tcgcggtgcc gacgacgacg
     2401 aacgcgccga acacggcgta cgcccacgcc ggaatcacga ggccttgctc gtagaagttc
     2461 gtgaacggca gctgcacgtg cagccgcacg tcggtgtgca tcacgttcga gtagaacagc
     2521 cacgccatcg ccgcgccggc gaacaggaac tcgagcagca ggcgcagctt gccggacagg
     2581 ccgtctttgc gcttcgtcag cttcgcgtag tcgtcggcaa agccgatcgc gccgaacgcg
     2641 atcgtgaccg aaatcgcgag ccacacgagc tggctgcgca ggtcgcacca gagcagcgtc
     2701 gacaccgcga ggcagaacag gatcagcgag ccgcccatgg tcggcgtgcc ggccttcttc
     2761 ttgtgcgtcg ccgggccgtc gtcgcggatc ttctcgccaa cattgcgtga cttgagccgc
     2821 tcgatgaacc acggaccgat cagaaacgac agcagcagcg cggtgatcgc ggcgcacagg
     2881 atgcgcgtcg atggatagcg gaacacgcgc agccagccga tcttgtcggc gaatgctccg
     2941 tagaacagat ggaacaacat ctacggcgtt tgctccttca ttacttcgag cacgcgctcc
     3001 agcttcatgc cgcgcgatgc tttgagcagg atccacccac ccgctgattc tgtacgtgcg
     3061 agcgcgcgct cggcggccgc ggcgtgcgac ggcgaaagct ccgcgtcttt accggcggcg
     3121 tcgacgatgt acttcgccat ctcaccgatt ccaacgacgc cgatgccgag cttgttcgcg
     3181 agctcgccga tttctttgtg cgcagccggc gcgtgatcgc cgagctcgag catgtcgccg
     3241 agcactgcga tcgcgggcaa tccgtgcgcg cgttcggcga gcgagtgcag tgctgcggtc
     3301 atcgacgcgg ggttcgcgtt gtagcaatcg acgatcacct tgcgaccgcc gagctcgatc
     3361 acttcgccgc gcatcgacac cggcagcgcg cgagagaggc cggcgagtgc ctgcgcgctc
     3421 gatgcaccgg cggcatgggc tgcggcgagc gcggcgcacg catcgatcgc tgcgtgttta
     3481 ccgacgagct ggagcttcag ctcgtgcttg tcgccaaata cgtcgagctg gcagatcgcg
     3541 ccagcgtccg tcggcttgta ctcgatgaga cggacgtcgg catcgccgtc accaaacgtg
     3601 acgtgacgtg cggtcggctg gtgctggcgc gcccagtact cgaggcgttg gtcgccggcg
     3661 ggcagcacga ccgtgccacc tgggcgcaga ccgagccaga tttccgactt cgcctgcgcg
     3721 atcgcatcgg tcgagccgag cagctcgatg tgcgcggagc cggcgttgac gacgactgca
     3781 acgtcgggct cggcgatctt cgtgaggtat tcgatctggc cgaggccgcg catgcccatc
     3841 tcgacgacgc cgtactgatg gaacaggcgc aggccgagta gcgtgagcgg caagccggtc
     3901 tcgttgttga gcgagccttc ggccgcgtgc gtggtgcctg ccatcgcgag cgcgcagcgc
     3961 gtcagctcct tcgtcgtcgt cttgccggcc gagccggtga tcgcgacgag cttgcgcgtg
     4021 tcactcgccc acgcgcggcg atgcgagcgc gcgatcttgc cgagcgccag ccgtgtgtcg
     4081 tcgacgacga tcgcggcgtt gcgcagcgac cgcagctccg gtgcgagcag atcgggcgcc
     4141 gcggcgggcg cccagcgctt accttccttg tcgaccatca cggcggtcgc gcccgccttg
     4201 acggcgtcga ggcagaagcg atgaccgtcg tgctgttcgc cgcgaatcgc gacgtagagc
     4261 gcgccgggcg cgacgacgcg actgtcgatc acgacgcggt cacagttcac gttgccgtcg
     4321 ccgagtagct caccaccggc atcgcgcgcg agaccggcga cgaggcgctg ctcgcgttcg
     4381 gcggcagcgg cagcggcttc ttcgcgatcg tcgaagtgga tcttcgtcgt gccgaggatc
     4441 tgatagtcct cgtgaccctt gccggcgatc acgacgacat cgccgggcgt tgcttcggcg
     4501 atcgcggcgc gaatggcagc gcgccgatcg acgtcgacga agaacggctt aggcacggcg
     4561 ggcaggatct gatcgaggat cttgcgcggg tcctcggtgc gcgggttgtc gctcgtaacc
     4621 accgcaagat cggcaagctc cgcgacggca gcgcccatct tcggccgctt cgtcggatcg
     4681 cgatcgccgc cacagccgaa cacgcagatc aggcggcggc gggtcagcgg acgcagcgcg
     4741 gtgagcacgt tgcgcagcgc atcgggcgtg tgcgcgtagt cgacgaggat gtcgaggtcg
     4801 gcaccgttca cgacgcgctc gacgcggccg ggcacacctt tgagctcggc gatgccggca
     4861 gcgatcgcgt cgtgcgggat cttgagtgcc tcggcgatgc cgactgcgag cgcgaggttc
     4921 gcgacgttgt actgaccgat gagcggcttc gcttcgatcg gcacggcgcc ggttggcgtc
     4981 gcgatcgagg cgtgaatgcc tttgaccgtc gattcctgga acgtgacgcg gatgtcggag
     5041 agccgaccct cgaccgaaac gcgcagcgtc gacgtgtgac gtggatccgc ggcgccggcc
     5101 atgccctcgc cttccggatc atcgacgttg acgacggcgg tgccaccctt gaggtgctcg
     5161 gcgaacagcc gatgcttcgc ggcgcggtac gcctccatcg tgccgtgcac gtcgaggtgg
     5221 tcctgcgtga ggttcgagaa cccgccgacg gcgaacgtga gtccgacgac gcgatcgagc
     5281 gcgagcgccg acgacgtcac ttccatcacg acgtgcgtgc agccggcctc gcgcatcttc
     5341 gcgaacgcgc catgcagcag ctgcggggtc ggcgtcgtgt agctcgcgga ttcgctgccg
     5401 ccgggccaac gcacctcgac ggtgccgatg acgccgggct tgtggccggc cgccaggagg
     5461 atgctctcga cgagatacgt cgtcgtggtt tttccgttcg tgccggtgat gccgatcaac
     5521 gtcatgccgg cggccggatt gccgagcgaa cgcgcgacga gcgggccgag cgcacggctc
     5581 gtcgacggca ccacgatctg cggcactttg acatcgagct cgcgctcgac gacgacagcg
     5641 atagccccct gctgcacgac gctgggcacg aacgcatgac cgtcgctgcg cattcctttg
     5701 atcgccacga acacgtcgcc aggctgcacg gtgcgcgaat c
//
LOCUS       DASUFX010000837         7704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_2082269, whole genome shotgun sequence.
ACCESSION   DASUFX010000837 DASUFX010000000
VERSION     DASUFX010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082269"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFV99_33635"
     CDS             complement(<1..760)
                     /locus_tag="VFV99_33635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064382.1"
                     /translation="MSTGVPKQIGKYQILDRVAVGGMAELFKAQLIGQLGFEKLVAIK
                     KILPHLAGDQTFVEMFIDEARITAQLDHKHIVSVFELGTDADTPYIAMQYVDGLDVLA
                     LLRECARAQIRLTPDLAAVIARDVLDALDYAHNAIDVHGRPLKIVHRDISPGNVLLSW
                     RGDIKLTDFGIARAAERRHKTEAGTLKGKYGYMSPEQVSGAEVDARSDLFSVGILLAE
                     MVMARRLFTSTNDLDILLMVRDARLDRLHKYAQEF"
     gene            940..1830
                     /gene="gspC"
                     /locus_tag="VFV99_33640"
     CDS             940..1830
                     /gene="gspC"
                     /locus_tag="VFV99_33640"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEX5064383.1"
                     /translation="MINNALHWVSAGLRRTWLVALAAVIACSVFAAHGVNSLVEASYL
                     DTAPSRSVPLHPVQATTPVQTPPDPSAFVSRNMFCSTCTETDGGGPGPTDSYHPPAIL
                     IATSVGSDPRATLRVPASEVQGSWGLGDDVPGVGRIERIGFTSIDVADGNRRGTLTLF
                     DGIPGGRSDAGAATPAPAAADPFADRVRKIDDHTFEVDRSLVRDLVGGAVGTSGARIS
                     PITKDGKLAGLRLFGVRPGSIASAIGLRNGDVLQAVNDTQIESANTMLELYAQLEKLN
                     NVELAGTRGGKPMALQLRLR"
     gene            complement(1827..3212)
                     /locus_tag="VFV99_33645"
     CDS             complement(1827..3212)
                     /locus_tag="VFV99_33645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064384.1"
                     /translation="MRAWPWLLIVCACGDGLPDRDDGAHSGSRLRVAWFDVDGTRQWQ
                     PEEGHQYESGRFVNSERFYDKLRDESCAPEPWLDGVTRCTPDVELIARKVFADAACTV
                     RVTQNPQSYRYVGDYDDKCGQRRMWHVFPVVSTMQSASYYIAGDGKCYLLPGMTEIGV
                     LGDELAYDTFATIDTLTTTNGERITIRYRKSQDGMVLPVSLYDATLGTSDLVRDIDGR
                     DVAKPRVAAPASPADMYYDDPSCTHGYVRGDIGCGPPQAALGDADYYAIGDHATASPR
                     YTLTAGSCMQQPIPPAGEFYEVLTATPLALAPVAISVANDETHRIELEHATAPSLALR
                     MRELFDTTLATECKPRQFPDEVYRCAPDELAAQIVPGTYSDPACTIALDLAMVVPEHR
                     QPRFVLQDRSLLGGTVTIYEIGELYTQPYYAFGGAMGTECMPVTPPAHLHALGRVVDW
                     SKLATATLATD"
     gene            3138..4982
                     /gene="murJ"
                     /locus_tag="VFV99_33650"
     CDS             3138..4982
                     /gene="murJ"
                     /locus_tag="VFV99_33650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5064385.1"
                     /translation="MCAVVAVGQTVAAGANDQQPRPRAHRRRGYARIAATCRALLAVL
                     ASAKENAAMPDVTPPRDESEAVRPGVSPARSRRSGGAVAVAAGIFLSRIAGLVRDRLF
                     AHYLGLSNAQDVFRAALRIPNYLQNLLGEGVLSASFIPVYARLLAEHREADATRVARA
                     VGTLLVLLASLLTLAGVLAADLLVSVIVPGWHGEKRELTVTLVRILFPGVALLVMSAW
                     CLGVLNSHRKFFLSYASPVLWNAALIATTIVAGRRLAGHDDDIAVWLAWGAVIGSAAQ
                     FLVQLPTVILLLRDLRPSLAVRDEGVQKTLRAFVPIFLGRGSVQISAFIDSMLASYLG
                     KGIIGAMSNAQTLYTLPVSLFGMAVSAAELPEMSSATGDEAARHEHLQTRLRSALRRV
                     VFLVVPSAIAFIAIGGPIVALIFQGGRFTASDTTIVWIILAGSAIGLSGGTQGRLLGS
                     AFYALGDPKPPLHAALVRVAITGVLGYAFALPIRNWLGSDVAVDVGPLHLVGRVGGYS
                     PTWGAFALTASAGFAAWVEFLLLDRWLSRRIGKVPIPVRLGFGALAASVVAGAAGFGG
                     AQLADHLGAKHWVAALVAIPVFGVVYLGIMVAARVPEISAITRRFFRR"
     gene            5024..5842
                     /locus_tag="VFV99_33655"
     CDS             5024..5842
                     /locus_tag="VFV99_33655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064386.1"
                     /translation="MPAVRYAFRARFAIAAALPLALLLAALSLGGLVTNAYARETPAW
                     LEQAIGQDWFDLVIAAPWIAICGIGARGSPRWRVLLAGAYAYTVYELFIYVFAVHFNA
                     LFLLYCATLGVAAFGLIAVLGELRDDTLRIDRRGARIGGGWLVTVGVLFGLLWLVEDV
                     PAVLHNMPPKSLVATGLLTNPVHAIDFAFVLPAHVVAGVLLWRGERTGQVYGPVLLAF
                     GVLMSASIAAMLIVVAARGGGAPTPVIAAMTLVTAATAAVLARTLHARPKLALS"
     gene            complement(5857..6213)
                     /locus_tag="VFV99_33660"
     CDS             complement(5857..6213)
                     /locus_tag="VFV99_33660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064387.1"
                     /translation="MANEKAVLFIGANRPIPGKENEAMRLWMETAGWLDGQAKQGWFT
                     RWDGCFLTAHGGDLNSMFICYGDRAKLDEWRRTDTFEAWVFRAMTCMEGLGVIPGVTM
                     AAAKETMERRSRAIGQ"
     gene            complement(6306..6992)
                     /locus_tag="VFV99_33665"
     CDS             complement(6306..6992)
                     /locus_tag="VFV99_33665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064388.1"
                     /translation="MRPIWIATCALAVIGAFFVVLQAAWMLKVQAEWLPYPAQVLGAA
                     LAGLVVSRAAAGSIRAAFVAGAAAVIVLALISYALPRAFTLTAVRSPHALIVVPILVI
                     ASGAACALAARVPAPPSAVWLPMSAAFVAACTIQLGGRLAHVAGLPAEPTALVAFGIA
                     AALVAGLNIRALVRSECAREVAIGVLALMLWGTLAQVVLRSAAALTIWDVLLLVGAPL
                     GAAIGARVRI"
     gene            complement(7048..>7704)
                     /locus_tag="VFV99_33670"
     CDS             complement(7048..>7704)
                     /locus_tag="VFV99_33670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064389.1"
                     /translation="NALDQLRTNEVALMGSRLPADGFVAGRQVWELREFHLTPTDIAP
                     HTMNLEPARDFDIARPAFVAPEGTRSGELVTYLEANATAVLASRHVLAPGMSANSSLV
                     GASPYGAWGKLTNPSPPEIPSQGVPHALDGVAIDVRDSFALNTCAGCHRHETDTRQFM
                     HVTFAAATDPTSSDGTVVLSSFLQSEIMPSGARFEDFSDLLVTKRVDLRDKPGLRSCR
                     "
BASE COUNT         1179 a         2588 c         2561 g         1376 t
ORIGIN      
        1 gaaactcctg cgcgtacttg tgcaggcgat cgaggcgcgc gtcgcgaacc atgagcagga
       61 tgtcgaggtc gttcgtcgac gtgaacagcc ggcgcgccat caccatctcg gcgagcagga
      121 tgccgaccga gaacaggtcg ctacgcgcat cgacttcggc gcccgacact tgctcgggag
      181 acatgtagcc gtacttgccc ttgagcgtgc ccgcctcggt cttgtggcgg cgctcggcgg
      241 cgcgcgcgat gccgaagtca gtgagcttga tgtcgccgcg ccacgacagc agcacgttac
      301 cgggcgagat atcgcgatga acgatcttga gcggccgacc gtgcacgtcg atcgcgttgt
      361 gtgcgtaatc gagcgcatcg agcacgtcgc gcgcgatcac ggccgcgaga tccggagtca
      421 ggcgaatctg cgcgcgggcg cattcgcgga gcagtgcaag cacgtcgagg ccatcgacgt
      481 actgcatcgc gatgtacggc gtatcggcat cggtgccgag ctcgaacacc gacacgatgt
      541 gcttgtggtc gagctgcgcc gtgatccgcg cctcgtcgat gaacatctcg acaaacgtct
      601 ggtcgcccgc gaggtgcggc aggatcttct tgatcgcaac cagcttttcg aagccgagct
      661 ggccgatcag ctgcgccttg aacagctcgg ccatacctcc caccgcgacg cgatcgagga
      721 tttggtattt cccgatctgc ttgggtacgc cggtgctcaa gaggtcctca tcatacagtc
      781 tgccgacgac tgcggtgccg ggctgccagt ttggcagctt tgccagcgcc aggctcgtct
      841 acgtggcgcg atccgacatg acaacgacgg gacattaatc gatggtccgc gcgcttacac
      901 gatggcacgc gtgttgatgt tgaaacgctg caaccagcga tgatcaacaa cgcacttcat
      961 tgggtctccg ctggccttcg tcggacctgg ctcgtcgcac ttgcggcggt gatcgcgtgc
     1021 tcggtatttg ccgcacacgg ggtcaattcg ctggtcgaag cgtcgtactt ggacacggct
     1081 ccctctcggt cggttcctct tcatcctgtc caagccacga ctcccgtcca aacaccgcca
     1141 gatccgtccg cgttcgtgtc gcgcaacatg ttttgctcga cgtgcaccga gacggatggc
     1201 ggcgggcctg gcccgacgga ttcgtatcac ccacccgcga tcctcatcgc gacgagcgtc
     1261 gggagcgatc cccgcgcgac gttgcgcgtc ccagcgtccg aagtgcaagg cagctgggga
     1321 cttggcgacg atgtgcccgg cgtcggtcgc atcgaacgga tcggctttac gtcgatcgat
     1381 gttgccgatg gcaatcgccg cggcacgctt acgttgttcg atggcattcc cggcggccgt
     1441 agcgatgcgg gtgcggcaac acctgcgcct gcggccgccg atccattcgc ggatcgcgtg
     1501 aggaagatcg acgaccacac gttcgaggtc gatcgcagcc tcgtgcgcga cctcgtcggt
     1561 ggcgcggtcg gcacgagcgg cgcgcgaatc tcgccgatca cgaaggacgg caagctcgcc
     1621 ggtcttcgcc tgtttggcgt gcgccccgga tcgatcgcga gcgcgatcgg cctacgcaac
     1681 ggtgacgtgt tgcaagcagt caacgacacg cagatcgaat cggccaacac gatgctcgag
     1741 ctctacgctc agctcgagaa gctgaacaac gtcgagctcg cgggcacgcg cggtggcaag
     1801 ccgatggcgc tgcagctgcg cttgcgttaa tcggtcgcga gcgtcgcggt tgcgagcttg
     1861 ctccagtcga cgacacgccc gagcgcgtgg agatgagccg gcggagtgac gggcatgcac
     1921 tcggtgccca tggcgccgcc gaacgcgtag tacggctgcg tgtagagctc accgatctcg
     1981 tagatcgtga cggttccgcc gagcagcgag cggtcttgca acacgaaccg cggctggcgg
     2041 tgttccggca cgaccatcgc gaggtcgagc gcgatcgtgc acgccggatc ggagtacgtg
     2101 ccaggcacga tctgagcggc aagctcgtcg ggcgcgcagc gatagacctc gtcgggaaat
     2161 tgccgcggct tgcactcggt ggcgagcgtg gtgtcgaaca gctcgcgcat gcgcagcgcc
     2221 aggctcggcg cggtcgcatg ctcgagctcg atccggtgcg tctcgtcgtt cgcgaccgaa
     2281 atcgcaacgg gtgcaagcgc gagcggcgtc gcagtcagca cttcgtagaa ttcgccggcc
     2341 ggtggaatcg gctgctgcat gcagctcccc gccgtgagcg tgtaccgcgg tgacgccgtc
     2401 gcgtggtcgc caatcgcgta gtagtccgcg tcgccgagcg cagcctgcgg cgggccgcag
     2461 ccgatgtcgc cgcgcacgta cccgtgcgtg cacgacgggt cgtcgtagta catatcggca
     2521 ggcgaagccg gcgcagcgac gcgcggcttg gccacgtccc ggccgtcgat gtcgcgcacg
     2581 agatcgctcg ttccgagcgt cgcgtcgtac aggctcacgg gcagcaccat gccgtcctgg
     2641 ctcttgcgat agcggatcgt gatccgttcg ccgttcgtcg tcgtgagtgt gtcgatcgtc
     2701 gcgaacgtgt cataggcgag ctcgtcgccg agcacgccga tctcggtcat gcccggcagc
     2761 aggtaacact tcccatcgcc cgcgatgtag tagctcgcgc tctgcatcgt gctgacgacc
     2821 gggaacacgt gccacatgcg acgctgcccg cacttgtcgt cgtagtcgcc gacgtaacga
     2881 tagctctgag ggttctgcgt cacgcgaacc gtacaggccg cgtcggcgaa taccttgcgc
     2941 gcgatcagct cgacatcggg cgtgcagcgc gtgacgccgt cgagccacgg ctcgggcgca
     3001 cagctctcgt cacggagttt gtcgtagaag cgctcgctat tgacgaagcg accgctctcg
     3061 tactgatgcc cttcctcggg ctgccactgg cgcgtgccgt ccacgtcgaa ccaggcgacg
     3121 cgcagccgcg atccgctgtg tgcgccgtcg tcgcggtcgg gcaaaccgtc gccgcaggcg
     3181 caaacgatca gcagccaagg ccacgcgcgc atcgtcgtcg aggatacgcg agaatagcgg
     3241 cgacctgccg cgcgttactt gccgtgttgg catctgccaa ggagaatgcg gcgatgcccg
     3301 acgtcacgcc tcctcgcgat gagagcgagg ccgtgcgtcc aggcgtttcg cccgcgcgct
     3361 cgcgtcgctc tggtggcgca gtagccgtcg cggccggcat ctttctgtcg cggatcgcgg
     3421 ggctcgttcg cgatcgtttg ttcgcgcact acctcggcct gtcgaatgcg caggatgtgt
     3481 ttcgagcggc gctgcggatc ccgaactact tgcagaacct gctcggcgag ggcgtgttgt
     3541 cggcgtcgtt cattccagtc tacgcgcggc tgctcgccga acatcgcgaa gcagatgcaa
     3601 cgcgcgtagc acgcgcagtc ggcacgctgc tcgtgctgct cgcatcgcta ctgacgctcg
     3661 ccggtgtgct cgcggccgat ctgctcgtca gcgtgatcgt gcccggatgg cacggcgaga
     3721 agcgtgaatt gacggtcacg ctcgtgcgca tcctgttccc cggcgtcgcg ctcctcgtga
     3781 tgtcggcgtg gtgcctcggc gtgctgaaca gtcatcgcaa gttctttctc agctacgcgt
     3841 cgcccgtgct gtggaacgcg gcactgatcg cgaccacgat tgtcgccggc cgccgactcg
     3901 caggacacga cgacgacatc gcggtctggc tcgcgtgggg cgcagtgatc ggctcggctg
     3961 cgcagttcct cgtccagctg ccgacggtca ttctgttgtt gcgcgacctg cggccgtcac
     4021 tcgcggtgcg tgacgagggc gtacagaaga cactgcgcgc gttcgtgccc atcttcctcg
     4081 gccgcggctc cgtccagatc tcggcgttca tcgatagcat gctcgcgagc tacctcggca
     4141 aaggcatcat cggcgcgatg agcaacgcgc agacgctcta cacgctgccg gtcagtttgt
     4201 ttggcatggc ggtgtcggcg gccgagctcc ccgagatgtc cagcgcgacg ggcgacgagg
     4261 ccgcgcgtca cgagcatctc cagacccggc tgcgcagtgc gctgcgccgc gtggtgttcc
     4321 tcgtcgtgcc gtcggcgatc gcgttcatcg cgatcggcgg tccgatcgtc gcgttgatct
     4381 ttcagggcgg ccggttcacc gcgagcgata ccaccatcgt gtggatcatc ctcgcgggct
     4441 cggcgatcgg gctctcgggc ggcacgcaag gtcgcctgct cggctcggca ttctacgccc
     4501 tcggcgatcc gaagccgcca cttcacgcgg cactcgtgcg cgtcgcgatc actggcgtgc
     4561 tcggctacgc gttcgctctg cccattcgca actggctcgg ctccgacgtt gccgtggatg
     4621 tcgggccgct tcacctggtc ggacgtgttg ggggctactc gccaacgtgg ggcgcgttcg
     4681 cgctcacggc cagcgctggc ttcgcggcgt gggtggaatt cttgctgctc gatcgctggc
     4741 tcagtcgccg catcggcaag gttccgatcc cggtgcggct cggattcggc gcactcgctg
     4801 cgtcggtcgt cgccggagcc gccggttttg gcggggcgca gctcgccgat catctcggcg
     4861 caaaacactg ggtcgccgcg ctcgtcgcga ttcctgtgtt cggcgtcgtc tatctcggca
     4921 tcatggttgc cgcgcgcgtg cccgagatca gcgcgatcac gcggcgcttc ttccgccgct
     4981 agcgcgtccc gcacccggca cgtcggatgc accagcattg aacatgccgg ctgttcgcta
     5041 cgcgtttcgc gcacggtttg cgattgctgc cgcgttgccg ctcgcgttgc tgctcgccgc
     5101 gctctcgctc ggtggtctcg tgacgaacgc atacgcgcga gagacaccgg cgtggctcga
     5161 gcaggcgatc ggccaggatt ggttcgatct cgtcatcgct gcgccatgga tcgcgatctg
     5221 cggtatcggc gctcgtggct caccgcgttg gcgcgtgctg ctcgccggtg catacgcgta
     5281 cacggtctac gagctgttca tctacgtgtt cgcggtccac ttcaacgcgc tgttcttgct
     5341 ctactgcgcg acgctcggcg ttgccgcgtt cggtctgatc gcggtgctcg gcgagctccg
     5401 cgacgacacg ctgcggatcg atcgccgcgg cgctcgcatc ggcggcggct ggctcgtcac
     5461 agtcggcgtc ctgttcggcc tcttgtggct cgtcgaagat gtgccggcgg tgttgcacaa
     5521 catgccgccg aagtccctcg tcgcgaccgg tctgctcacg aacccggtgc atgcgatcga
     5581 cttcgcgttc gtgctgcccg cgcacgtggt cgccggcgtc ctcttgtggc gcggcgagcg
     5641 cacgggccag gtctacggtc ccgtgctgct cgcgttcggc gtgctgatgt cagcgtcgat
     5701 cgccgcgatg ctgatcgtgg tcgcagcacg cggcggcggc gcgccgacac cggtgatcgc
     5761 agcaatgact ctcgtcaccg ccgcgaccgc ggccgtgctg gcgcggacgc tgcacgcgcg
     5821 cccgaagctc gcgctgagct gacgcgactt gtcgacctac tggccgatcg cgcgcgaccg
     5881 gcgttccatc gtctccttcg cggccgccat ggtcacgccc gggatcacgc caaggccttc
     5941 catgcaggtc atcgcgcgga acacccacgc ctcgaacgtg tcggtccgcc gccactcgtc
     6001 gagtttcgcg cggtcgccgt agcagatgaa catcgagttc agatcgccgc cgtgcgcggt
     6061 caagaagcaa ccgtcccagc gcgtgaacca gccctgcttg gcctgaccgt cgagccagcc
     6121 tgcggtttcc atccacagcc gcatcgcctc gttctctttg cccggaatcg ggcggttcgc
     6181 accgatgaac agcactgcct tttcgttcgc catggttcct tcctcctctg agttggaact
     6241 aggcgatcgc ggtccgctcc cggaatacgt agtcggacgc aatctcccgc ggctcacgct
     6301 ggacgctaaa tacgaacgcg cgcgccgatc gcggcgccga gcggcgcgcc gacgagaagc
     6361 agtacatccc agatcgtgag cgcggctgcc gaacgcagga cgacctgcgc gagggtgccc
     6421 cacagcatca acgcgagcac accgatcgcg acctcacgcg cgcattcgct gcgaacaagc
     6481 gcgcggatgt tcagaccagc aacgagcgcc gcggcgattc cgaacgcaac cagcgcggtc
     6541 ggttcggcgg gcaagccggc gacatgcgcg agccggccac cgagctggat cgtacacgca
     6601 gcgacgaacg cggcggacat cggcaaccac actgcgctcg ggggcgccgg cacacgcgcg
     6661 gcgagcgcgc acgccgctcc gctcgcgatc acgaggatcg gaacgacgat cagcgcgtgc
     6721 ggactgcgca ccgccgtcag cgtgaacgcg cgcggtaacg cgtacgagat gagcgcgagc
     6781 acgatgactg ccgcggcgcc ggcgacgaac gcggcgcgga tcgatcccgc ggcagcgcgc
     6841 gagacgacga gtccggcgag cgctgcgccg agtacctgcg cggggtacgg cagccactcc
     6901 gcttggacct tcagcatcca cgcggcctga aggacgacga agaacgcccc gatcaccgcc
     6961 agcgcacatg tcgcaatcca tatcggtctc atcgcggacg atgggagcac gagcctgtgc
     7021 acgtcgcaac gcgaaccgtg ctcgcgctca acgacaggat cgcaggcccg gcttgtcgcg
     7081 caggtcgacg cgcttggtga cgagcaggtc actgaagtcc tcgaagcgcg cgccgctcgg
     7141 catgatctcg ctttgcaaga agctcgatag cacgacggtg ccgtcgctcg atgtcgggtc
     7201 agtcgccgcg gcgaacgtca cgtgcatgaa ctggcgcgtg tcggtctcgt ggcggtggca
     7261 gccggcgcac gtgttgagcg cgaacgagtc gcgcacgtcg atcgcgacgc cgtcaagcgc
     7321 gtgcggaacg ccctgcgacg gaatctccgg cggactcgga ttcgtcagct tgccccacgc
     7381 accgtacggc gacgcgccga cgagcgatga gttcgcgctc atgccgggcg caagcacgtg
     7441 acgcgacgcg agcactgcgg tcgcgttcgc ttcgagatac gtaacgagct cgccgctgcg
     7501 cgtgccctcc ggcgccacga acgccggccg cgcgatgtcg aagtcgcgcg cgggctcgag
     7561 gttcatcgtg tgtggcgcga tgtccgtcgg ggtcaggtgg aactcgcgca gctcccagac
     7621 ctggcggccc gcgacgaacc cgtcggcagg caaccgggat cccatcagcg cgacctcgtt
     7681 cgtgcgcagc tgatcgagcg cgtt
//
LOCUS       DASUFX010000838         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_414468,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000838 DASUFX010000000
VERSION     DASUFX010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414468"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFV99_33675"
     CDS             <1..205
                     /locus_tag="VFV99_33675"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5064390.1"
                     /translation="PGFYSVDSKPYATIFIDDKSFGETPLFKVQLPAGKHRIRAQRAD
                     GKSQRLSITIQPGKLFSSGTLSW"
     gene            205..1140
                     /locus_tag="VFV99_33680"
     CDS             205..1140
                     /locus_tag="VFV99_33680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064391.1"
                     /translation="MRWLVVLLLVILAMTRGARADNPSVAAARKAVDEIRYDDAQALL
                     VQALKQGANRPDVLVEIYRLSAATAAVLGPTDLAEQYYRRMIALDPDSTLPPDASPRL
                     REPFVAAQAYMAAQQRFEARATRTAQGIEVSVVDPLGMVVAIATLDGGELRAKQPYAG
                     QAITLQDVGDRVVLLDESGNFLREIPLPARQEVDEPVGTSTPSTPFFRRWYVYAVPTV
                     AFAGATAFLFVDAQRAKDRLDDIIANNGGHFYDDAETERRRYRNETIAAWVGAGITVG
                     FATTAIIMAATRPRAVTVTPSVGADHASVQLEAKF"
     gene            1212..1892
                     /locus_tag="VFV99_33685"
     CDS             1212..1892
                     /locus_tag="VFV99_33685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064392.1"
                     /translation="MKKLRAVLFPLIVMVGCTVGSSSEPTTSAATSYNLIDTDNDGVP
                     DCLDLDNDMQCDVGLGSVCMDPLVDTNNDGIPEGLDLDCDGTIDITWCQQPLVDSDND
                     GVPDGLDLDCDGTADVSLPGPGTQCAPELVDANNDGVPEGIDTDCDGQIDVPFNFCVP
                     AAVDSNSDGVPDGLDTDCDGQIDVPFNNPPTPPQNPTCNPLVDSNMDGVPDGIDVDCD
                     GTVDYTFP"
     gene            complement(1992..2345)
                     /locus_tag="VFV99_33690"
     CDS             complement(1992..2345)
                     /locus_tag="VFV99_33690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064393.1"
                     /translation="MHRLALVAILLAGCSTDDDLGIEIITPASGTTIASTGPIVFELK
                     IHGDLHPGEADIAVDGTRVPPSQVQGACINDNGSSQVTWLPLAIANGLHEVEVICVYD
                     LSEYGDKITLMFAQP"
     gene            complement(2345..>3179)
                     /locus_tag="VFV99_33695"
     CDS             complement(2345..>3179)
                     /locus_tag="VFV99_33695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEX5064394.1"
                     /translation="IVLNSPAQWRKHRAVIERADRSAGLRVNHEHQEAEVALYDPAGV
                     CSRLGTTRANVTAADLDGLDGLHFHSLCQNNSDALERAVAAFETKFSDFIDRMKWINF
                     GGGHHITRPDYEIDRLVRVIREFRGRHPKVQVYLEPGEAIALGTGVLVAQVMDTFHNG
                     MDIAILDTSATAHMPDVLEMPYRPVIVDGADPGVKKHTYRLGGMTCLAGDVIGDYSFD
                     KPLAIGDKLVFLDMAHYTMVKTTTFNGVRLPSIATHDPATKRITVHRRFGYTDYRDRL
                     S"
BASE COUNT          538 a         1043 c         1045 g          553 t
ORIGIN      
        1 gcccggcttc tacagcgtcg acagcaagcc gtacgcgacg attttcatcg atgacaaatc
       61 gttcggtgag acgccgctgt tcaaggtcca gctacccgct ggcaagcacc ggatccgtgc
      121 gcagcgcgct gatggcaaat ctcagcgact ctcgatcacg atccagcccg gcaagctgtt
      181 cagctcgggt acgctgtcct ggtaatgcgc tggctcgtcg ttttgctgct cgtgatcctc
      241 gcgatgacgc ggggcgcgcg cgccgacaat ccgagcgttg ctgccgcacg caaggccgtc
      301 gacgagattc gttacgacga tgcgcaagcg ttgctcgtgc aggcgctcaa gcagggtgcg
      361 aaccgtcctg acgtgctcgt cgagatctac cggttgtcgg ccgcaacggc cgccgtgctt
      421 ggcccgacgg atcttgccga gcagtactac cggcgcatga tcgcgctcga ccccgactcg
      481 acgttgccgc cggatgcatc gccgcgcctg cgcgagccat tcgtggccgc gcaagcgtac
      541 atggccgcgc agcaacgctt cgaggcacgt gcgacgcgga cggcacaggg catcgaggtc
      601 agcgttgtcg atccgctcgg catggtggtc gcgatcgcga cgctcgacgg cggcgagctc
      661 cgcgcgaagc agccctacgc cggtcaggcg atcacgctcc aagacgtcgg cgatcgcgtc
      721 gtgctgctcg acgagagcgg caacttcctg cgcgagattc cgctgcccgc gcggcaggag
      781 gtcgacgagc cggtggggac gtccacgccg agcacaccgt tttttcgccg ctggtacgtc
      841 tacgcggttc cgaccgtggc gttcgccggt gcgaccgcgt ttctgtttgt cgacgcgcag
      901 cgcgcgaagg atcggctcga cgacatcatc gcgaacaacg gcggtcactt ctacgacgat
      961 gccgagaccg agcgccggcg ctatcgcaac gagacgatcg ccgcgtgggt cggcgcggga
     1021 atcacggtcg ggtttgcgac caccgcgatc atcatggcgg cgacccgccc acgcgccgtg
     1081 acggtgaccc cgtcggtcgg ggccgaccat gcgagcgtgc agctcgaggc taagttctag
     1141 ccctgtaagg gcttttgcgt gtagtcgggc tgttgtcggg tgattggtac ccgcgggtgc
     1201 aacctggctt catgaaaaag ctacgcgcgg ttctgttccc cctcatcgtc atggtcggct
     1261 gtacggtggg cagcagcagc gagcccacga cgagcgcggc gacgtcgtac aacctgatcg
     1321 acaccgacaa cgatggcgtg ccggactgcc tcgatctcga caacgatatg caatgcgatg
     1381 tcgggctcgg ctcggtctgt atggacccgc tcgtcgacac gaacaacgac ggcatccccg
     1441 agggcctcga cctcgactgc gatggcacga tcgacatcac gtggtgccag cagccgctcg
     1501 tcgatagcga caacgacggc gtgcccgacg gcctcgatct cgactgcgac ggcaccgcgg
     1561 acgtttcgct ccccggtccg ggcacgcagt gcgcgcccga gctcgtcgac gcgaacaacg
     1621 acggcgtgcc cgaaggcatc gacaccgact gcgacggcca gatcgacgtg ccgttcaact
     1681 tctgcgtccc cgcggcggtc gacagcaaca gcgatggcgt gcccgacggt ctcgacaccg
     1741 actgcgacgg gcagatcgac gtgccgttca acaacccgcc gacgcccccg cagaacccga
     1801 cgtgcaaccc gctcgtcgac agcaacatgg acggcgtgcc tgacggcatc gacgtcgact
     1861 gcgacggcac ggtcgactac acgttcccgt gagcgactgt ccgggagtgt ccaacgtaac
     1921 cccggtacgt ttggcgcaag ctcgcgcagc tccagctgtc cggctgtccg gttttgtccg
     1981 aatttgtccg gctacggctg cgcgaacatc agcgtgatct tgtcgccgta ctcggacagg
     2041 tcgtagacgc agatgacctc gacctcgtgg aggccgtttg caatcgcgag cggcaaccac
     2101 gtgacctgcg acgagccgtt gtcgttgata cacgcgcctt gcacctggct aggcggaacc
     2161 cgggttccgt cgacagcgat gtccgcctcg ccgggatgca ggtcgccgtg gattttcagc
     2221 tcgaacacga tcggaccggt cgacgcgatc gtggtgccgc tcgccggcgt gatgatctcg
     2281 ataccgaggt cgtcgtcggt cgaacaccca gcgagcagga tcgcgacgag cgcgaggcga
     2341 tgcatcagct gagtctatcg cggtagtcgg tgtagccgaa ccggcgatgg acggtgatgc
     2401 gcttggttgc cggatcgtgc gtggcaatgc tcggcaaccg cacaccgttg aacgtggtgg
     2461 tcttcaccat cgtgtagtgc gccatgtcga ggaacacgag cttgtcgccg atcgcgagcg
     2521 gcttgtcgaa gctgtagtcg ccgatcacgt cgccggcgag gcacgtcatg ccgccgagcc
     2581 gatacgtgtg cttcttcacg ccggggtcgg cgccgtcgac gatcaccggc cggtacggca
     2641 tctcgagcac gtcgggcatg tgcgcggtcg ccgacgtgtc gaggatcgcg atgtccatgc
     2701 cgttgtggaa cgtgtccatc acctgcgcga cgagcacgcc ggtgccgagc gcgatcgctt
     2761 cgccgggctc gaggtagacc tggaccttcg ggtggcggcc gcggaactcg cggatcacgc
     2821 gcacgagccg atcgatctcg taatccgggc gcgtgatgtg atgaccgccg ccgaagttga
     2881 tccacttcat gcggtcgatg aagtcggaaa acttcgtctc gaacgcagca accgcgcgct
     2941 cgagcgcgtc actgttgttc tgacacagcg agtgaaagtg cagaccgtcg aggccgtcga
     3001 ggtcggccgc cgtcacgttc gcgcgtgtcg tgccgagccg actgcacacg ccggccgggt
     3061 catacagcgc gacctcggct tcctggtgct cgtggttgac acgcagaccc gccgagcgat
     3121 cggcgcgctc gatcacagcg cgatgcttgc gccactgcgc cggcgaattg agcacgatg
//
LOCUS       DASUFX010000839         9433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_831809,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000839 DASUFX010000000
VERSION     DASUFX010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831809"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFV99_33700"
     CDS             <1..292
                     /locus_tag="VFV99_33700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064395.1"
                     /translation="NKGANDLLDKIQTLREELAEYRSRAGELHAQLVTLKVVKTGGEL
                     MTSLKNRLTETSDRMQKATLAIVDTQEKLMLMRLKFGNQLADLHLSDAIANN"
     gene            complement(295..1233)
                     /locus_tag="VFV99_33705"
     CDS             complement(295..1233)
                     /locus_tag="VFV99_33705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5064396.1"
                     /translation="MKRIVVPAGRSKSVPLLFGPAVLAACLSMSCKVQLTPESIAKSW
                     HAYQDAKKDLTPENEYYVGRSVATNLLSRANYKYVEADAYQSGELGKTTAYVNAVGNV
                     LAAAAMGMPRKDDRPSPIAGWHFVIVDDDTINAFAASGGFILINKGLVKAAKNEDELA
                     AVLAHEIAHTVRGHAIGSIKKSRMAGVYKEMLNNTVQLDEQQLGTLTQAFEGAMTDMI
                     DSMVVKGYSRDTEFEADRVGLKIMADAGYDPQAFIRFLDGLEKKMGGAGSGGFKSTHP
                     SPKDRIAKLKAEAAKIASVKAPGVRTERFQAAIATL"
     gene            complement(1230..1688)
                     /locus_tag="VFV99_33710"
     CDS             complement(1230..1688)
                     /locus_tag="VFV99_33710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064397.1"
                     /translation="MKRVLLVLLLGAGVALAAPKVGSSVTIRVLSAKVMKSPKFIGAS
                     AGSVSRGDQLMVKEVKGDWYRVEGAQSGWIHKTNVTEGKVALSSKPGGSGGNVNRDEV
                     ELAGRGFTPEVEQKYKQSNPNLDFSHVDAIEKTSVDPAELEAFAKEGQLP"
     gene            complement(1689..4190)
                     /locus_tag="VFV99_33715"
     CDS             complement(1689..4190)
                     /locus_tag="VFV99_33715"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5064398.1"
                     /translation="MSRGLSARTRSLLSGVGVGVIVGIVAAGFAITRPAVLERPEAAT
                     YDQRARAVMKPSNASKDIVLIDIDESDIEDAENNFDVLWPWPRSLYGDITTYCKKAGA
                     KAIVFDWLFQDRGGMGVADDEQFAEATHAAGNAVFGLALTTNKLVDRPLEGPFAARLA
                     GYETRDEAKAIALRLAAWNVRSFVLMRAGKFELWYGGKKTAEDVEKTWKRLVSVDELK
                     PLFVPETPEAPEGQEPKEGEPPPEAAEPAPPKIVKLDPNELSQELTVVSIIKDRDGIP
                     LAGKFPRREGLDPPLAIIAAAPARLGNVYQNPEADGIMRRHAPLVLNGNVAYPSLSLA
                     AYLVAHPNVTPKIDGHVLDLGERKIQLDDDGQITMHFHGSHVYPHLSAYEVLRSSVML
                     EEGTKPSVPFDALKGKYVMVSATGQALRDLRATPISTRHLGAEVQATALDNMLNGDVV
                     RRAHPWLDGAIAFVISVIVSLLMIVLWRVIRKPGLALIATAAVTTVILVGYYFIARAA
                     LGGANVWIAYAAPSIGGGVSAFATLLALSAVERSGKRFVQEALGRYTSTALVRELMEH
                     PEYLSLEWGESREMSVYFSDIAGFTTISEGLKPEDLVALLNDYLTIMTDLVLSHGGVV
                     DKYIGDAIMAFWGAPLPAADHALRATKCAIAMRKKCDELRAGWHQKFGHEVFARAGVN
                     SGNAVVGNMGSKHKYNYTVMGDMVNLASRLEGANKAYGTFLMISETTVAKLGGAMDVR
                     ELDRIAVKGKDVPVTVYEVLDEVGVTDPAWIARARKFEDGLAKYRAKDFAGAIEIFEA
                     NAGDEPSEIFAARCKHFLEEPPPDNWNGVWVMKEK"
     gene            complement(4310..4495)
                     /locus_tag="VFV99_33720"
     CDS             complement(4310..4495)
                     /locus_tag="VFV99_33720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064399.1"
                     /translation="MLALPRSSHWLPLKICFGHALFEAKQPRRDLVDAVAQRLRRNGI
                     ALVQLPLEMETNDFSCK"
     gene            4562..5986
                     /gene="lpdA"
                     /locus_tag="VFV99_33725"
     CDS             4562..5986
                     /gene="lpdA"
                     /locus_tag="VFV99_33725"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5064400.1"
                     /translation="MPEQRFDAVVIGAGPGGYPAAIRLGQLKVKTAVIEREYFGGVCL
                     NVGCIPSKAVIHAAKMYEKMSHADDLGIQLASKPTLDMGKLQTWKGGVVNKLTSGVRT
                     LLKGNGVTMIEGSAKLEKSGPDGHRITVTGKNGTETIIAKNIVLATGSRPFEIPGFKI
                     DQKRIIDSTGALALDAVPPRMIVIGGGYIGLELGMVYAKFGSKVTVVEALPRVLASMD
                     KDCVAVVERKLKKMGVEVMANTKAKSWEDKGDRAVLTVELQDGKTATIDADKILLSIG
                     RRPNSENLGLEAAGVAFDKKGYVIADDHLRTNVPGIYAIGDLIGGMMLAHKATKEGEV
                     VAEIIAGHKAAFDVRTIPAVVFTDPEIASTGLTEDEAKAKGHAQLKVGKFPFAALGRA
                     LSVNDTEGFVKVVSDGKTGELLGLHIVGNGAGDLISEGALAIEMGAVVDDLRLTIHPH
                     PTLSEAIMEAAAAALGEAVHVINR"
     gene            6036..6671
                     /gene="lipB"
                     /locus_tag="VFV99_33730"
     CDS             6036..6671
                     /gene="lipB"
                     /locus_tag="VFV99_33730"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5064401.1"
                     /translation="MPSWQVLDLGTADYREVWAKQLALVEARQIGQAPDTLIVVEHPH
                     VFTLGRRRDSQQNVLAPGDVPVIEIERGGDVTYHGPGQLVAYPIVFLDGEERDLHKFL
                     RNMEEAVIRTCARAGVTADREPGKTGVWTTTELGRKKLCSMGIACRKWVTFHGLALNV
                     TSDLSYFARINPCGFEASVMSSLDKLVPGVDIAAVRRVLVEELGTALDRSA"
     gene            complement(7141..8628)
                     /locus_tag="VFV99_33735"
     CDS             complement(7141..8628)
                     /locus_tag="VFV99_33735"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEX5064402.1"
                     /translation="MRTWLTLILSSLCLLAAACGPDKRDDSCFGSDCMSGTCESGQTR
                     ECYTGQKDTIGVGPCATGAQTCTASGQWGNCVGEVVPAAETCGDGIDNNCDGGTDNDV
                     DLDGDGFTTCGGDCCDSTECGTPGAVNPSAFDAPGNGFDDDCNGSVDDAIALCDQGLS
                     SGSTDGMDFAKAIDICQQATMADKKWGVINATLTFPDGTGTPDPNSHSIRPHFGTKAM
                     PRGGVNMALFSSGAAAGKGDTNPNYNDFVSYSSTNESGFPADFVAANGGTLPNAPGCP
                     DASGTTANDPVMLTLDIRVPSNAHSFSLKSNFYSAEFPEWTCSPYNDFFVVLLDSAYT
                     GSPANPMDKNLAFYTAPDMKNYPVGVNLAFGNTGLFTQCVNGSTGCSGTAGTISTCVS
                     TDDLMGTGFDDPSSFSCDANSLKGGATGWLTTSGNVTPGEIIKLRIAIWDTSDHAFDS
                     LAIVDGFAWSVDSTQPGTVILRADQPNNPKAVETTVPLMYQRNFK"
     gene            complement(8783..>9433)
                     /locus_tag="VFV99_33740"
     CDS             complement(8783..>9433)
                     /locus_tag="VFV99_33740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064403.1"
                     /translation="LHPRVAYQGLLSEEGAATRVAAALPALSRFRHDYAGAISVVDWD
                     HRLPSNSLTLRIYGYYGEDTLDAGYEAFDDRLDQIAERDKYPEFDVPDFDGLAADEAY
                     EIELSPAGQVGRCRLTSAWRRTVASRDAQTAVGLVQTCDEYQKLVSASPSRPTYLGDL
                     EAVSWTPPCESDHTRWTLDVWYLLAFDGRIGSGRSFLADLETKQIISVRDFSVRTG"
BASE COUNT         1636 a         3158 c         3032 g         1607 t
ORIGIN      
        1 caacaagggc gcgaatgacc tgctcgacaa gatccagacg ctgcgcgagg agctcgccga
       61 gtaccgctcg cgcgctggcg agctgcatgc tcagctcgtc acgctgaagg tcgtgaagac
      121 cggcggcgag ctgatgacgt cgctcaagaa ccgactcacc gagacgtccg accgcatgca
      181 gaaggcgacg ctcgcgatcg tcgatacgca ggagaagctg atgctgatgc ggctgaagtt
      241 cggaaaccag ctcgccgacc tccacctgtc ggacgcgatc gcgaacaact aggcttacag
      301 cgtcgcgatc gcggcctgga agcgctcggt gcgcacgccc ggcgccttca cgctggcaat
      361 cttcgctgcc tcggctttga gcttcgcgat ccgatccttc gggctcgggt gcgtcgactt
      421 gaagccgccg ctgcccgcgc cacccatctt cttctcgaga ccgtcgagga agcggatgaa
      481 tgcctgcgga tcgtagccgg cgtcggccat gatcttgagg cccacgcgat cagcctcgaa
      541 ctcggtgtcg cgcgagtagc ccttcacgac catcgagtcg atcatgtcgg tcatcgcgcc
      601 ttcgaacgcc tgggtcagcg tgccgagctg ctgctcgtcg agctgcacgg tgttgttgag
      661 catctccttg tagacgcccg ccatgcgcga cttcttgatc gagccgatcg cgtggccacg
      721 caccgtgtgc gcgatctcgt gcgcgagcac ggcggccagc tcgtcctcgt tcttcgcggc
      781 cttcaccagg cccttgttga tgaggatgaa accacccgac gcggcgaacg cattgatcgt
      841 gtcgtcgtcg acgatgacga agtgccaacc ggcgatcggc gacggccggt catccttgcg
      901 cggcatgccc atcgcggcgg cagcgagcac gttgccgacg gcgttgacgt acgcggtcgt
      961 cttgccaagc tcgcccgact gatacgcatc cgcttcgacg tacttgtagt tcgcgcgcga
     1021 gagcaagttc gtcgcgaccg agcggccgac gtagtactcg ttctccggcg tcaggtcttt
     1081 cttcgcgtct tgatacgcgt gccagctctt cgcgatcgac tccggcgtga gctggacctt
     1141 gcacgacatc gagagacagg cggcgagcac cgcgggcccg aagaggagcg ggacggattt
     1201 actccgaccg gcggggacga caatccgttt catggcagct gtccctcctt cgcgaacgct
     1261 tcgagctccg caggatcgac gctggtcttc tcgatcgcgt cgacgtgcga gaagtcgagg
     1321 ttcgggttcg actgcttgta cttctgctcg acctccggcg tgaagccgcg gccggcgagc
     1381 tcgacctcgt cgcgattgac gttgccgccc gagccacccg gcttcgacga caacgcgacc
     1441 ttgccctcgg tcacgttcgt cttgtggatc cagcccgact gcgcgccttc gacgcgatac
     1501 cagtcgccct tcacctcttt gaccatgagc tgatcgccgc gcgacaccga tcccgcggat
     1561 gcgccgatga acttcggcga cttcatcacc ttcgccgaga gcacgcgaat cgtcaccgac
     1621 gagccgacct tcggcgccgc cagtgcgaca cccgcaccga gtagaagcac aagcaatact
     1681 cgtttcatct atttctcctt catcacccag acgccgttcc agttgtcagg cggcggttcc
     1741 tcgaggaagt gtttgcagcg cgcggcgaag atctcgctcg gctcgtcgcc ggcgtttgcc
     1801 tcgaagatct cgatcgcgcc cgcgaagtcc ttcgcgcggt acttcgcgag accgtcttcg
     1861 aacttgcgcg cgcgtgcgat ccacgccgga tcggtgacgc cgacctcgtc gagcacctcg
     1921 tacacggtga ccggcacgtc cttgcccttc accgcgatcc gatcgagctc gcgcacgtcc
     1981 atcgcgccgc cgagcttggc gaccgtcgtc tcgctgatca tgaggaacgt gccgtacgcc
     2041 ttgttcgcgc cctccagacg cgaggcaagg ttcaccatgt cccccatgac cgtgtaattg
     2101 tacttgtgtt tcgaccccat gttgccgacg acggcgtttc ccgaattgac gccggcacgc
     2161 gcaaagactt cgtggccaaa cttctggtgc cagcccgcgc gcagctcgtc acacttcttg
     2221 cgcatcgcga tcgcgcactt ggtcgcacgt agcgcgtgat cggcggccgg tagcggcgcg
     2281 ccccagaacg ccatgatcgc atcgccgatg tacttgtcga cgacgccgcc gtgcgacagc
     2341 acgaggtcgg tcatgatcgt gaggtaatcg ttgagcagcg cgaccaggtc ctcgggcttc
     2401 aggccctcgc tgatcgtggt gaagccggcg atgtcggaga agtacacgct catctcacgg
     2461 ctctcacccc actcgagcga caggtactcc gggtgctcca tgagctcccg gacgagcgcg
     2521 gtcgacgtgt agcgaccgag cgcctcttgc acgaaccgct taccgctgcg ctcgacggcc
     2581 gagagtgcga gcaatgtcgc gaacgccgag acgccgccgc cgatcgacgg tgccgcatac
     2641 gcgatccaca catttgcgcc tccaagcgcc gcccgcgcga tgaagtagta gccgacgagg
     2701 atcacggtcg tgaccgctgc ggtcgcgatc agcgcgagcc cgggcttccg gatcacgcgc
     2761 cacagcacga tcatcagcag cgacacgatc acgctgatca cgaacgcgat cgcaccgtcg
     2821 agccacggat gcgctcgccg cacgacgtcg ccgttgagca tgttgtcgag cgcggttgcc
     2881 tgcacctcgg cgccgaggtg ccgcgtcgag atcggcgtcg cacgcaagtc gcgaagcgcc
     2941 tggccggtcg ccgacaccat cacgtacttg cccttcagcg cgtcgaacgg caccgacggc
     3001 ttcgtgccct cctcgagcat cacgctcgac cgcagcacct cgtacgccga caggtgcgga
     3061 tacacgtgcg agccgtggaa gtgcatcgtg atctggccgt cgtcgtcgag ctggatcttg
     3121 cgctcgccga gatcgagaac gtggccgtcg atcttcggcg tcacgttcgg gtgcgcgacg
     3181 agatacgcgg cgagcgacag cgacggatac gcgacgttgc cgttgagcac gagcggtgcg
     3241 tgccggcgca tgatgccgtc ggcctcgggg ttctgataga cgttgccgag ccgcgcaggt
     3301 gccgccgcga tgatcgcgag cggcggatcg agaccctcgc gacgcggaaa cttgccggcg
     3361 agcggaatgc cgtcgcgatc cttgatgatc gagacgaccg tcaattcctg cgacagctcg
     3421 ttcggatcga gcttgacgat cttgggtggc gccggctccg ctgcctcggg cggcggttcg
     3481 ccttccttag gctcctggcc ttcgggcgct tccggcgttt ccggcacgaa cagcggcttg
     3541 agctcgtcga cgctgacgag ccgcttccag gtcttctcga cgtcttcggc ggtcttcttg
     3601 ccgccgtacc agagctcgaa cttgccggca cgcatcaaga cgaagctgcg cacgttccac
     3661 gccgcgagcc tgagtgcgat tgcctttgct tcgtcgcggg tctcgtagcc ggcgagccgc
     3721 gccgcgaacg gtccttcgag cggccgatcg acgagcttgt tcgtcgtgag cgccaggccg
     3781 aacaccgcgt tgccggcggc gtgcgtcgct tcggcgaact gctcgtcgtc ggcgacgccc
     3841 atgccgccac ggtcctgaaa cagccagtcg aagacgatcg ccttcgcgcc ggccttcttg
     3901 cagtaggtcg tgatgtcgcc gtagagcgag cgcggccacg gccacagcac gtcgaagttg
     3961 ttctcggcat cttcgatatc gctctcgtcg atatcgatca gcacgatgtc cttcgacgca
     4021 ttgctcggct tcatcaccgc gcgggcgcgc tggtcgtacg tcgcggcctc gggccgctcg
     4081 agcacggccg gccgggtgat cgcaaacccc gccgccacga tgccgacgat cacgcccacg
     4141 ccaacgcccg acagcaacga gcgtgtccgc gccgagagtc cccgcgacat cggtccacag
     4201 tcataacacg aggagtgcgg tcgcgaggct gttgctcccg ttcagacctg cgtagaagga
     4261 agggatgtcc tggccgacca ccatagagcc gtcggagctc agcgccgcgt catttgcaac
     4321 tgaagtcgtt cgtctccatt tcgagaggaa gctgcacaag tgcgatgccg ttgcgtctca
     4381 gacgctgcgc gaccgcgtca acgagatcgc gtcgcggctg cttcgcctcg aacagcgcgt
     4441 ggccgaagca aatcttcaac ggcagccagt ggctagaacg cggaagcgcc agcatcagcg
     4501 cacgcgccag cgacgtcgct aggcctctcc gtcgggtgcg cgggcgtggt aagacgcgcc
     4561 catgcccgag caacgtttcg acgcagtcgt gattggcgcc ggtcctggtg ggtaccccgc
     4621 cgcgatccgc ctcggtcagc tcaaggtcaa gaccgccgtt atcgagcgcg agtacttcgg
     4681 tggcgtgtgc ctcaacgtcg gatgcatccc gtcgaaggcc gtcatccacg ccgcaaagat
     4741 gtacgaaaaa atgtcgcacg ccgatgacct cggcattcag ctggcgagca agccgacgct
     4801 cgacatgggc aagctccaga cctggaaggg cggcgtcgtc aacaagctga cctccggcgt
     4861 ccgcaccctg ctcaagggca acggcgtcac gatgatcgag ggcagcgcga agctcgagaa
     4921 gtcgggcccc gacggtcacc gcatcaccgt gaccggcaag aacggcaccg agacgatcat
     4981 cgcgaagaac atcgtgctcg cgacgggctc gcggccgttc gagattccgg gcttcaagat
     5041 cgatcagaag cgcatcatcg attcgacggg cgcgctcgca ctcgacgcgg tgccgccgcg
     5101 catgatcgtg atcggcggtg gctacatcgg cctcgagctc ggcatggtgt acgcgaagtt
     5161 tggcagcaag gtcaccgtcg tcgaggcgct accgcgcgtg ctcgcgtcga tggacaagga
     5221 ctgcgtcgcc gtcgtcgagc ggaagctcaa gaagatgggc gtcgaggtca tggcgaacac
     5281 gaaggcgaag agctgggaag acaagggcga ccgcgccgtg ctcaccgtcg agctgcagga
     5341 cggcaagacg gcgacgatcg acgccgacaa gatcctgcta tcgatcggcc gccggccgaa
     5401 cagcgagaac ctcggcctcg aagcggcggg ggtcgccttc gacaagaagg gctacgtgat
     5461 cgccgacgac cacctgcgca cgaacgtgcc cggcatctac gcgatcggcg acctcatcgg
     5521 cggcatgatg ctcgcgcaca aggcgacgaa ggaaggcgaa gtcgtcgccg agatcattgc
     5581 cggccacaag gcggcgttcg atgtccgcac gattcccgcc gtcgtgttca ccgacccgga
     5641 aatcgcatcg accggcctca ccgaggacga ggcgaaggcc aaaggccacg cgcagctcaa
     5701 ggtcggcaag ttcccgttcg ccgccctcgg ccgtgcgctg tcggtcaacg acactgaagg
     5761 gttcgtcaag gtggtctccg acggcaagac cggcgagcta ctcggcctgc acatcgtcgg
     5821 caacggcgcc ggcgacctga tctcggaggg cgcactcgcc atcgagatgg gcgcggtcgt
     5881 cgacgacctg cggctcacga tccacccgca cccgacgctg tcggaagcga tcatggaagc
     5941 ggcggccgcg gcgctcggcg aagcggtcca cgtcatcaac cgctgacgca gcgctgcatc
     6001 gccgaaccgg cgaattcgtg gtaatcgcct gatcgatgcc ctcctggcag gtcctcgatc
     6061 taggcaccgc tgactaccgc gaggtatggg cgaagcagct cgcgctcgtt gaggcgcgcc
     6121 agatcggcca ggcgcccgac acgctgatcg tcgtcgagca tccgcacgta ttcacgctcg
     6181 gccgccggcg ggattcgcag cagaacgtgc tcgcgcccgg cgacgtcccg gtgatcgaga
     6241 tcgagcgcgg cggcgacgtc acctatcacg gccccggcca gctcgtcgcg tatccgatcg
     6301 ttttcctcga cggcgaagaa cgcgacctcc acaagttctt gcgcaacatg gaggaggcgg
     6361 tcatccgcac gtgtgcgcgc gctggcgtca cggccgatcg cgagccaggc aagaccggcg
     6421 tgtggacgac gacagagctc ggccgcaaga agctgtgctc gatgggtatc gcgtgccgca
     6481 agtgggtcac gttccacggc ctcgcgctca acgtgacgag cgacctgtcc tacttcgcgc
     6541 ggatcaatcc gtgcgggttc gaagcgagcg tgatgtcgtc gctcgacaag ctcgtgcccg
     6601 gcgtcgacat cgcggcggtc agacgcgtgc ttgtcgaaga gctcggtacc gcgctcgatc
     6661 gcagcgcgta attcgtactt gccaggcgcg cgcgaatcgg tagagtggaa atcgacgcgg
     6721 ccgcccgcgt cgatcatgga aaccactcgc tagaacccga ccagagcccg ccacggcggt
     6781 ctcatcctat tggttgcgtg ttcgtgagtg gcccgaccct tccaggagtg agtcgcgcgc
     6841 tgagcagcgc tgcgctcgcc gtcggtcatc gcgtgcacgc gcgcacgtca ccgcgggact
     6901 tgctcctgca tggagacctc tatgtccaag atcgatcttc gcgagctatt gcagcgcacg
     6961 ctcgctgcga atggaaacca gtccgtcgcc accacaacgc caaagccgaa accagcgaag
     7021 cccaaagcgc cgccgatgca tgcgaaccgc gcgttgcgac cacggcgccg gtcgtaggcc
     7081 acgccgaaaa aaagcaaagc ccccgaagct cgggggccct gccatcagca gcgcaacgcg
     7141 ctacttgaaa ttgcgctggt acatcagcgg caccgtcgtc tcgaccgcct tcgggttgtt
     7201 cggctgatcg gcgcgcagga tgaccgtacc cggctgcgtc gaatcgaccg accaggcgaa
     7261 gccgtcgacg atcgcgagcg agtcgaacgc gtggtcgctg gtgtcccaga tcgcgatacg
     7321 cagcttgatg atttcgcccg gcgtcacgtt gccgctcgtc gtgagccagc ccgtcgcgcc
     7381 gcccttgagg ctgttcgcat cgcaggagaa cgacgacgga tcgtcgaagc cggtgcccat
     7441 cagatcgtcg gtgctgacgc acgtcgagat cgtgcccgcc gtgcccgagc agccggtcga
     7501 accgttcacg cattgcgtga acaggcccgt gttgccgaac gcgaggttca cgccgaccgg
     7561 gtagttcttc atgtcgggcg ccgtgtagaa cgcgaggttc ttgtccatcg ggttcgccgg
     7621 actaccggtg tacgccgagt cgagcagcac gacgaagaag tcgttgtacg gcgagcacgt
     7681 ccactcgggg aactcggcgc tgtagaagtt gctcttgagc gagaagctgt gcgcgttcga
     7741 cggcacgcga atgtcgagcg tgagcatcac cgggtcgttg gccgtcgtgc cgctcgcgtc
     7801 cgggcagccg ggcgcgttcg gcaacgtgcc gccgttcgcg gcgacgaagt cggccgggaa
     7861 gcccgactcg ttcgtgctcg agtacgacac gaagtcgttg tagttcgggt tcgtgtcgcc
     7921 cttaccagcg gcggcaccgc tcgagaacag cgccatgttg acgccgccgc gcggcattgc
     7981 cttggtgccg aagtgcgggc gaatcgagtg cgagttcgga tccggcgtgc cggtgccgtc
     8041 gggaaacgtg agcgtcgcgt tgatgacgcc ccacttcttg tcggccatcg tcgcctgctg
     8101 gcagatgtcg attgccttcg cgaagtccat gccgtcggtc gagcccgacg acaggccctg
     8161 gtcgcagagc gcgatcgcat cgtcgacgga gccgttgcag tcgtcgtcga agccgttgcc
     8221 cggcgcatcg aacgccgacg ggttcaccgc gcccggcgtg ccgcactcgg tcgagtcgca
     8281 gcagtcgcca ccgcacgtcg taaacccatc gccatcgaga tcgacgtcgt tgtcggttcc
     8341 gccatcgcag ttgttgtcga tgccatcgcc gcacgtctcg gcggccggaa ccacttcacc
     8401 aacgcagttc ccccactgcc ccgacgcggt gcacgtctgt gcacctgtgg cgcatgggcc
     8461 aacaccaatc gtgtccttct ggccggtgta acactcgcgc gtttgccccg actcgcacgt
     8521 gcccgacatg cagtcgctgc caaagcatga gtcgtcgcgt ttatccggtc cgcatgccgc
     8581 tgcaagcagg cacagcgacg agagaatcaa ggtcaaccag gtgcgcatga agggcccttc
     8641 ctggcgcgga gaatagactc atcgcgagag gcgcgcgcaa gctcaaaata ccgagaccgg
     8701 ggatcccgga atgacacagg ctctccccgg tgaggcgccg gccgaggctc gccgaacccc
     8761 atttacgact tttggaagcc ggctaaccgg ttcgtaccga gaagtcgcgg accgagatga
     8821 tctgcttcgt ctccaagtcc gccaaaaagg accgaccaga gccgatgcgg ccgtcgaatg
     8881 cgagcaagta ccagacgtcg agggtccacc gggtatggtc gctctcgcag ggcggcgtcc
     8941 aggacaccgc ttcgaggtcg cccaggtacg tcggccgtga cggcgaggcc gagacgagtt
     9001 tttggtactc gtcgcaggtc tggaccagac cgaccgcggt ctgcgcatcg cgggacgcga
     9061 ccgtgcggcg ccacgccgag gtcagccggc agcgcccgac ctggccggcc ggcgacagct
     9121 cgatctcgta ggcttcatcg gcggcgaggc cgtcgaagtc ggggacgtcg aactccgggt
     9181 acttgtcgcg ctcggcgatc tggtcgagcc ggtcgtcgaa cgcctcgtag ccggcgtcga
     9241 gcgtgtcctc gccgtagtag ccgtagatcc ggagcgtgag gctgttcgac ggcagccggt
     9301 ggtcccagtc gacgaccgag atcgcgccgg cgtagtcgtg gcggaaccga gacagcgcgg
     9361 gcagcgcggc cgcgacgcgc gtcgccgccc cctcctcgga caacagcccc tggtaggcga
     9421 cgcgagggtg gag
//
LOCUS       DASUFX010000840        11291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_135199,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000840 DASUFX010000000
VERSION     DASUFX010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135199"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFV99_33745"
     CDS             <1..307
                     /locus_tag="VFV99_33745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064404.1"
                     /translation="NIGIADASRRKADVPLYTLRRNSNGQTVQTTDPGRALITGRWAD
                     IGKFKGPILRGLQMRPPFFHDGSAATLQDVINFYNSRFSIGLTPQEQADLLAFLQAL"
     gene            complement(328..1695)
                     /locus_tag="VFV99_33750"
     CDS             complement(328..1695)
                     /locus_tag="VFV99_33750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064405.1"
                     /translation="MLACLLATVAGCGDDDCCTSAYHDAGIDTALGQDANPSLTEVAL
                     IPAAATRDLDLLFVLDDSDTMFELQKKLTDTLPDFFQALLHAPGGLPNLHVGVVTTDL
                     GTQGSGDPAPGPAIPAAACAGVGKDGLLQTNGAPVTEGFLSSVLDSGGNRIKNYNGEL
                     VPVVQQMASVGATGCAFEQPLEAMRRGLTNPVNAGFLRPNARVAVVVMSDEDDCSLTY
                     ASLLRTDATALGPLGSFRCTRFGVACDGGGADPDQMNAVGTKTMCHSNEASPFLTPVG
                     RYTEFLATLKSDPRDVLFSAIVAPTTPFNVDSLLNPTTGMEQTLLMSACRYASSIGSL
                     GAAPAVRTSELAKRVAQGTTINVCDGDLPGQLLALATRVNGLLGTACIQHLINMPAAC
                     EAFDIDGAGNATFVPDCATTSARPCFQIEREPLKCPVQQALAVQIMRSSVASPDTWTS
                     VRCAI"
     gene            complement(1798..2334)
                     /locus_tag="VFV99_33755"
     CDS             complement(1798..2334)
                     /locus_tag="VFV99_33755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064406.1"
                     /translation="MPQPSGYYLALDKKAVARLRSTKGDRARRAFVVDFIRTSPARTL
                     CVDETHYLLEFVNRERDVPESLKWGKAMHKGQFYRLERLETAAVVAALAALPNDQAAL
                     AKQLRAMDEAKFRYHNVSFWVAEEWEKQGRKHVVLRDTPARAFAQLEDIRAFVGAVPS
                     DHTLFFAISYYDGGVLVG"
     gene            complement(2354..4048)
                     /locus_tag="VFV99_33760"
     CDS             complement(2354..4048)
                     /locus_tag="VFV99_33760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064407.1"
                     /translation="MRILVAVALAGCYSELATSPNDAPADAVGDSDVSTFAPTWSYTT
                     PDGFTQSPEEDIAITGSLQLGVRGFDQIASATAGTESLRIVPRRASLGPLPLLTEPLN
                     NRVLVWMLEPLGVIWHNPIVWQDGTSDAESRVLYHVVEGGLLDATWSHAVDGTALSIE
                     MTSPAHELATGLYELGALRVRVVTGTATLANRLLIVTPQLGSAQVLVYSDDVYDSAAV
                     DAAGALLTPLPGKVSERSGPVVEKHVRFQSQILGIDHVFGLGDTPSPNASPVLVADRG
                     ELYVGSPDGWRISALATKIIKTGPLGMRRMRYARAGSIGGQTFNFYVFSPRGTVTLWP
                     VGRITRPYRISQFGDVTLADFPGTDNDYSEDLPNGTNVMHTHTTWAWDASTLVEASTL
                     EANATLAHTGMEGNSVDGGWRHTATGTLTSFAVGGAQGFAPKTLGPTAYRYDTGDAAF
                     ALYSTFSVYHQQRAWSGAWADRPDRVAALQLASTIYFGSIKALHNTDVLPAGSKLTVS
                     QVLHAGDTTDDPAALHARYTLSEPLRDGMLDLDLDAKPQAPWDLAIAGDERGVVFQ"
     gene            4268..6508
                     /locus_tag="VFV99_33765"
     CDS             4268..6508
                     /locus_tag="VFV99_33765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064408.1"
                     /translation="MGKVDTGKTETIEEPQPAAVLARRPPELSDVHLPPRFTPRRVLG
                     AGAMGYVVEARDESLGRDVAVKVISARRVGDARSRERFLREARAFAALHHENIVTIHD
                     LDPRGHFIVMELVRGDTLRMRIERDKQLAPAEVRRIGAALLDALATAHAAGFVHRDVK
                     PANVLLGDDGSVKLADFGIAATTDSELTATGELLGTPAYMAPEQLRGHGNDPRADIYA
                     TGATLFEAATGERLHVGRERTEDPRVRILASVPDRALAEAIARAVREKPGERYANVGE
                     FARALAARVPPHRHLRMRLVAGGAVAVIAGIGTAVAMRSHEKPDELALGVAALERHEL
                     TAAEQHLAKAPDDARTQYYRALVGWWTQRPTDEVKGELDRAIAGGLDERSSAIARGVR
                     ELVELEYPGVVETFRTLRMKYPDDRYVLYGEFEALFHCGRPDDALDVFHHLREVAPHF
                     GLGANHALTFYLAKGDVESARWALAGTSGTEHVRWQARVDAAADDVAAAKRGLDKARA
                     AATGTDADTLGWDRVALDAQTGDVEHAHALALELATKNLQAGAVPLYGLALARGVTDG
                     ADGSQHLAGVALDAARLPPSILVSTRDAWLELATFSVIDGDVARARTALAALPTSVDD
                     KMLEVALARVLLSALVGDDGAVARFFPSAFPPVRAAAEALAAERSNDPRAAAAAWARA
                     LSSEGFGRFHIALALRLARAHAKAGDARTAAEVCASIIHPTLFHWSWAAAVGECRQLT
                     AARTPP"
     gene            complement(6483..7799)
                     /locus_tag="VFV99_33770"
     CDS             complement(6483..7799)
                     /locus_tag="VFV99_33770"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5064409.1"
                     /translation="MAGDEHPVTTAAVVRENALAIRRFVLTVVAGPNAGARVVSTGQR
                     VVIGTHASAELALTDATVSRFHCELTMGDGAITVRDLGSKNGTRINGLSIVEAHLEGT
                     ATLLLGRTEVEIQLADSFAELPLSSRESYGELVGKSPAMRALYAQLERAAASDVTVLL
                     LGETGTGKELAARAIHEHSARADAPFTIVDCGAIPHSLIDGELFGYERGAFSGAERSR
                     PGVFELAAGGTVFLDEIGELPSELQPKLLRVLEEREVQRLGAPKPTSVEGRVIAATNR
                     NLRADVNAARFRADLFYRLAVLEVNLPPLRERLDDIPLLVDSFLRANALVDSPDAAAL
                     RDPAWRADLARQGWRGNVRELRNHLERALLAPAEVVPGAGWEAPPEIDTNQKLAAVRE
                     QWVRYVERRYIVDLLQKHDGNVSAAARAAGVDRAHFYRIMAACGLR"
     gene            8010..9041
                     /locus_tag="VFV99_33775"
     CDS             8010..9041
                     /locus_tag="VFV99_33775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5064410.1"
                     /translation="MPRTLKETNETLVIELPVSGPPPVPQPSLRPAGTLPKPPRRLAA
                     GTIPPVSRSKRRAPKELGIGSRIGPWLIERELGRGGMGRVYAVAHSGFGKRAALKVCH
                     TSMIDRDLAANTFLREARIVNLIDHPAVPDVFATGTFENRPYLAMERLQGETLRQLWS
                     SGALSQESALEILVELCGVLQAAHDAGVVHRDLTLDNVFVLDKPGAGGCRIKLLDWGF
                     ARVLAEDDPLRGHIAGTMTYVAPEAIVGREVSPASDVYALGVLAYILLLGQAPFAADE
                     LVELAAMHLHQEPPAPQRLRPAIRPELEQLLLAMLAKEPSWRPVLAEIQRELSARPRR
                     RRRWLQLVP"
     gene            9245..9991
                     /locus_tag="VFV99_33780"
     CDS             9245..9991
                     /locus_tag="VFV99_33780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5064411.1"
                     /translation="MNLADDQFRLLVESVQDYAIYLLDPSGLVRSWNAGAERLKGYTA
                     EEAIGRSLATFFIPDDRASGKPQQLLAAALKFGRYEDSGWRVRKDGSQFWANAVITAL
                     FEDGRAAPAPLSPTALGGIRIVLVDDDEDTRQAIADALASVGASVEQAVSASDAVQRL
                     QLNRPDVLLSDIGMPLEDGYALIRRVRSLAPDLGGDVPAIALTAYARHEDATKAEASG
                     FQLHVSKPVALEDLIDAIRSCLPSRSGTAR"
     gene            10065..10673
                     /locus_tag="VFV99_33785"
     CDS             10065..10673
                     /locus_tag="VFV99_33785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064412.1"
                     /translation="MRTSLVVIVLLLACSKSSSEQQAGSAAPPAASGSSGSSAAPAGS
                     GSSAAPVEAATVVGPTRSVSGELDVSGLMSGKFQWIKKDQTSPISCAWSVEKEIGGLH
                     VDLSDGAGHLLKMTIDVPPTEAGVPRLDVTSTGLPAPLKAKLGFNMSGEETGKFAVKF
                     DTTLTENPDAEPAKKPGKKDEKPSGPSLTIKGTLEVTCPPKK"
     gene            10713..11246
                     /locus_tag="VFV99_33790"
     CDS             10713..11246
                     /locus_tag="VFV99_33790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064413.1"
                     /translation="MPNDPDSHGSPDAAKVRLICSMCSQPYFATAGTNPGECVSCMLG
                     RAEAEARQAAHERKQAGRRALASRITNRVGARLIVGVIVLAIGGVLVAWQAVQHAREK
                     AAERHRANASANVYVIKTENWKWRMCHCEDFACAAQVKADFEAWTAKEAAPDDADVKR
                     AAKSATDSLLHCYDQLR"
BASE COUNT         1936 a         3722 c         3769 g         1864 t
ORIGIN      
        1 caacatcggt atcgccgatg cgtcacgtcg aaaggcggac gtgccgctgt acacgttgcg
       61 acgcaactcc aacgggcaga ccgtgcagac gaccgacccc ggccgcgcgc tgattaccgg
      121 gcgatgggcc gacatcggaa agttcaaagg cccgatcttg cgagggctgc agatgcggcc
      181 tccgttcttt cacgacggct ctgccgcgac gctgcaggac gtgatcaatt tctacaactc
      241 gcggttctcg atcggactca cgccgcaaga acaggccgac ctgctcgcgt tcctacaagc
      301 gctctgatcc gaggcgtagc gagccgatca gatcgcgcag cgcacgctcg tccaagtgtc
      361 ggggctcgcc accgacgagc gcatgatctg aactgcgagt gcttgctgaa cggggcattt
      421 gaggggttcg cgctcgatct ggaagcacgg ccgcgcgctg gtcgtcgcgc agtcgggaac
      481 gaacgtcgcg ttgcctgcgc catcgatgtc gaacgcctcg catgcggcgg gcatgttgat
      541 caggtgttgg atgcaagcgg tcccgaggag cccgttcacg cgcgtggcaa gcgcgagcag
      601 ctggccgggt agatctccgt cgcacacgtt gatcgtggtg ccctgagcaa cgcgcttggc
      661 gagctccgat gtgcgcaccg ccggcgcggc acctagcgaa ccgatcgagg acgcgtacct
      721 gcacgcgctc atgaggagcg tttgctccat gcctgtcgtc ggattgagga gcgaatcgac
      781 gttgaacggg gtggtcggcg cgacgatcgc gctgaacagc acatcacgcg gatcagactt
      841 gagcgtcgcg agaaactcgg tgtaacgccc caccggcgtc agaaacggcg atgcctcgtt
      901 cgagtggcac attgtcttgg tgccaaccgc attcatctgg tccggatcgg caccgccacc
      961 gtcgcacgcg actccaaacc gcgtgcagcg aaacgaaccg agcggtccga gtgcggtcgc
     1021 gtcggtgcgc agtaggctcg catacgtcaa cgagcagtcg tcctcgtcgg acatcacgac
     1081 gacggcgacg cgcgcgttcg ggcgtaagaa acccgcgttg accggattcg ttagcccgcg
     1141 acgcatcgcc tcgagtggct gctcgaacgc gcaacctgtc gcgccgacgc tcgccatctg
     1201 ctgaacgacc gggacgagct cgccgttgta gttcttgatg cggtttccgc ccgagtcgag
     1261 gacgctgcta aggaacccct cggtcaccgg cgcgccattc gtttgcagca ggccgtcctt
     1321 gcctacgcct gcgcatgccg ccgccggaat cgcggggccg ggcgctggat caccgctgcc
     1381 ctgtgtgccg aggtcggtgg tcacgacgcc gacgtggagg ttcggcaagc cgcccggcgc
     1441 gtggagcagc gcctggaaga agtccggcaa cgtgtccgtg agcttcttct gcagctcgaa
     1501 catcgtgtcc gaatcgtcga gcacgaacag cagatcgagg tcgcgcgtgg cggctgccgg
     1561 gatcaatgcg acctcggtca ggctcgggtt tgcgtcttgg cccaaggcgg tgtcgatgcc
     1621 agcatcgtgg tacgcgctcg tgcaacagtc gtcatcaccg cacccggcga cggtggcgag
     1681 aaggcaagcc aacatggcgt actgcatggg gcaaggttag tgcggcgtgc ccggcgaatc
     1741 cgtcacgcaa catatctttt gttcacatgg ccgaggcgga cggtgcgccg actcgcgtca
     1801 cccgacgagc acaccgccgt cgtagtagct gatggcgaag aacagtgtgt gatctgacgg
     1861 gactgcgccg acgaaggccc gaatgtcctc gagctgagca aacgcgcgcg ccggcgtatc
     1921 gcggagcacc acgtgtttgc gcccctgctt ctcccattcc tcggcgaccc agaacgaaac
     1981 gttgtgatag cgaaacttcg cttcgtccat cgcgcgcagc tgctttgcga gcgcggcctg
     2041 atcgttcggt agcgcggcga gtgcggccac gacggccgcc gtctccaatc gctcgagccg
     2101 gtagaactgg cctttgtgca tcgccttgcc ccacttcaaa ctctcgggca cgtcgcgctc
     2161 gcgattcacg aactcgagca ggtaatgcgt ctcgtcgacg cacagcgtcc gggctggtga
     2221 ggtgcgaatg aagtcgacga cgaaggcgcg gcgcgcgcga tcacctttcg tcgaccgcag
     2281 cctcgctact gctttcttgt cgagtgcgag gtagtagccc gaaggttgcg gcacgccggc
     2341 atcgtaccgc gcatcattga aacacgacgc ctcgttcgtc gcccgcaatg gcgaggtccc
     2401 atggcgcctg cggcttcgcg tcgagatcga gatcgagcat gccgtcccgc agcggctcgc
     2461 tgagcgtgta gcgcgcgtga agcgccgcag gatcgtcggt ggtgtcgccc gcgtggagca
     2521 cctggctgac cgtgagcttg ctaccggcgg gcaagacgtc ggtgttgtgg agtgccttga
     2581 tgctgccgaa gtagatcgtc gatgcgagct gcagtgcagc gacgcgatca gggcggtcgg
     2641 cccacgctcc gctccatgcg cgttgctggt gatagaccga gaacgtcgag tacagcgcga
     2701 acgctgcgtc gcccgtatcg tagcgatagg ccgtgggccc tagtgtcttt ggggcgaagc
     2761 cttgcgcacc gccaaccgca aacgacgtca gcgtgccggt cgcggtgtgg cgccagccgc
     2821 cgtcgacgga gttaccttcc atgcccgtgt gcgcgagcgt ggcattcgcc tcgagcgtag
     2881 aggcctcgac gagtgtcgac gcgtcccagg cccacgtcgt atgcgtgtgc atcacgttcg
     2941 tgccgttcgg cagatcctcg gagtaatcgt tgtcggtgcc ggggaagtcg gcgagcgtga
     3001 cgtcgccaaa ctgactgatg cgatacggtc tcgtgatgcg cccgaccggc cacagggtga
     3061 ccgtgccgcg gggcgagaac acgtagaagt tgaacgtctg gccgccgatc gagccagcgc
     3121 gcgcgtaacg catgcgccgc atcccgagcg gtccggtctt gatgatcttc gtcgcaagcg
     3181 ccgagattcg ccagccgtcg ggcgagccga cgtacagctc gccgcgatcg gcaaccagca
     3241 ccgggctcgc gtttggcgat ggcgtgtcgc cgagcccgaa cacgtgatcg atgccgagga
     3301 tctggctctg gaaccgcacg tgcttttcga caacgggccc gctgcgctcc gagactttgc
     3361 cgggcagtgg ggtgagcaga gcgcccgccg catcgaccgc tgcgctgtcg tagacgtcgt
     3421 cgctgtagac aaggacctga gcgctgccga gctgcggcgt gacaatgagc agccgattcg
     3481 cgagcgtcgc ggtgccggtc acgacgcgta cgcgcaacgc cccgagctcg tacaggccgg
     3541 tcgcaagctc gtgcgccgga gacgtcatct cgatcgatag cgccgtgccg tcgacggcgt
     3601 gcgaccacgt cgcatcgagc aggccacctt cgacgacgtg gtagagcacg cgactctcgg
     3661 catcggacgt gccgtcttgc cagacgatcg ggttgtgcca gatcacgccg agtggctcga
     3721 gcatccagac cagcacgcga ttgttcaggg gttcggtgag cagcggcagc ggcccgagcg
     3781 atgcgcgacg cggcacgatt ctcaggcttt cggtccccgc agttgcggac gcgatctgat
     3841 cgaaaccgcg cacgccgagc tgcaggctac cggtaatggc gatgtcctcc tcgggagact
     3901 gcgtgaagcc gtcgggcgtc gtgtagctcc acgtcggcgc gaacgtggac acatcgctgt
     3961 cgccaaccgc atcggccgga gcgtcgttcg gtgaagtggc gagctcgctg tagcaaccgg
     4021 cgagcgcgac ggcgacgaga atgcgcatgg ctcgtgccct cagcaaccgc tgtacctggc
     4081 ccgccgcacc gagatccaag gagttcgccg ctggctgtag actagcgcgc gacagtggca
     4141 agcgtcgacg tcaccccgcg gagccgaaac ggcgcagaac gcgcgaaccg gatgcggcac
     4201 gatcgttgct caacgggccg gcatgaccac caccgatagg ctccaagcac cgtagtactg
     4261 tggctccgtg ggcaaggtcg acaccggaaa gacggagacc atcgaggagc cgcaacctgc
     4321 ggccgtgctc gctcgccgtc cgcccgagct cagcgatgtc cacttgccgc cgcggttcac
     4381 gccgagacgc gtgctcggcg cgggtgcgat gggctacgtc gtcgaggccc gcgacgaatc
     4441 gctcggccgc gatgtagcgg taaaggtcat ctcggcacgg cgcgtcggcg acgcccgcag
     4501 tcgcgagcgg ttcttgcgcg aggcgcgcgc gttcgcggcg ctgcaccacg agaacatcgt
     4561 cacgatccac gatctcgatc cgcgcggtca cttcatcgtg atggagctgg tccgcggcga
     4621 cacgctgcgc atgcggatcg aacgggacaa gcagctcgca cctgccgagg tgcgccgcat
     4681 cggtgccgcg ctcctcgatg cactcgcgac cgcgcacgct gcgggcttcg tacatcgcga
     4741 cgtcaaaccg gcgaacgtac tgctcggcga cgacggctcc gtcaagctcg ccgacttcgg
     4801 tatcgcagcc acgacggaca gcgagctgac cgcgaccggc gagctcctcg gcacgcctgc
     4861 gtacatggca cccgagcagc tgcgtggcca cggcaatgat ccacgcgccg acatctacgc
     4921 gaccggcgca acgctgttcg aggcggcaac cggtgaacgg ttgcacgtgg gccgcgagcg
     4981 caccgaggat ccgcgcgttc gcattctcgc cagcgtcccc gaccgcgccc tcgccgaggc
     5041 gatcgcgcgt gccgtgcgcg agaaaccggg cgaacgctac gccaacgtgg gcgagttcgc
     5101 gcgcgcgctc gccgcccgcg tcccaccgca tcggcatctc cgcatgcggc tcgttgccgg
     5161 cggcgctgtc gcagtaatcg ctgggatcgg caccgcggtc gcgatgcgct cgcacgagaa
     5221 gcccgacgag ctcgcgctcg gcgttgctgc actcgagcgc cacgagctca ccgcggccga
     5281 gcaacacctc gccaaggctc ccgacgacgc gcgcacgcag tactaccgcg cgctggtcgg
     5341 ttggtggacg cagcgcccga ccgacgaagt gaagggcgag ctcgatcgcg caatcgcagg
     5401 tggtctcgac gagaggtcat ctgcgatcgc acgcggtgta cgcgagctcg tcgagctcga
     5461 gtatcccggt gtcgtcgaaa cgttcagaac gctacgcatg aaatatccag acgaccgcta
     5521 cgtgctgtac ggcgagttcg aagcgctgtt tcactgcggt cgacccgacg acgcgctcga
     5581 cgtgttccac cacttgcgcg aggtcgcgcc gcacttcggg ctcggcgcga atcacgcgct
     5641 cacgttctat ctcgcaaagg gcgatgtcga gagcgcgcgc tgggcgctcg ccggcacgtc
     5701 aggcaccgag cacgtgcggt ggcaagcgcg cgtcgacgcg gctgccgatg atgtcgcagc
     5761 agcaaagcgc ggcctcgaca aagcacgtgc agccgcgacc ggcacggacg ccgacacgct
     5821 cggctgggac cgcgtcgctc tcgacgcgca gaccggcgac gtcgagcacg ctcacgcgct
     5881 tgcactggag ctcgccacga aaaacctgca agcaggcgca gtaccgctct acgggctcgc
     5941 gctcgcacgc ggagtcaccg atggcgccga cggctcgcag caccttgccg gagtcgcact
     6001 cgacgccgcg cgcctaccgc catcgatctt ggtctcgact cgcgacgcgt ggctcgagct
     6061 cgccacgttc agcgtgatcg acggcgacgt cgcacgcgca cgaaccgcac tcgctgcact
     6121 accgactagc gtcgacgaca agatgctcga agtcgcgctt gcgcgcgtgc tgctcagcgc
     6181 acttgtcggc gatgacgggg cagtcgcaag gttcttcccc agcgcctttc cgcccgtgcg
     6241 tgccgctgcc gaagcacttg ctgccgagcg gagcaacgat ccgagggccg ccgccgccgc
     6301 gtgggcccgg gcgttgtcgt ccgagggctt cgggcgattt cacatcgcgc ttgcgcttcg
     6361 gctcgcacgg gcacacgcga aggccggcga tgcgcgcacc gcggccgagg tctgcgcatc
     6421 gatcattcat ccgacgctgt tccactggtc gtgggcagcg gccgtcggcg aatgccgtca
     6481 gctcaccgca gcccgcacgc cgccatgatc cgatagaaat gcgcgcgatc gacgccggcg
     6541 gcgcgcgcag ctgcgctcac gttgccgtcg tgcttctgca acagatcgac gatgtagcgg
     6601 cgctcgacgt agcggaccca ttgctcgcgc actgcggcga gcttctgatt cgtgtcgatc
     6661 tccggcggtg cttcccaacc cgcacccggc acgacctcgg ccggcgcgag cagggcgcgc
     6721 tcgagatgat tgcgtagctc gcgcacgttg ccgcgccacc cctggcgtgc gaggtcagct
     6781 cgccaggccg gatcgcgtag tgctgctgcg tcgggactgt cgacaagcgc gttcgcgcgc
     6841 aaaaacgaat cgacgagcag cggaatgtcg tcgagtcgct cgcggagcgg cggcaggttc
     6901 acttcgagta ctgcgagtcg ataaaacagg tccgcgcgga accgcgctgc gttgacgtcc
     6961 gcccgcaagt tacgattcgt cgctgcgatc acgcgcccct cgacggacgt cggcttcggc
     7021 gcgcccaggc gctgtacttc gcgctcctcg agcacgcgca atagcttcgg ctgcagctcg
     7081 ctcggcagct cgccgatctc gtcgagaaac acggttccac ctgccgcgag ctcgaacacg
     7141 ccgggccgcg accgttcggc gccgctgaac gcgccgcgct cgtacccgaa tagctcgcca
     7201 tcgatcagcg aatgcggaat cgcgccgcaa tcgacgatgg taaacggcgc gtcggcgcgt
     7261 gcgctgtgct cgtggatcgc gcgggctgcg agctccttgc ccgttccagt ttcgccgagc
     7321 agcagcacgg tgacgtcgct cgccgcggcg cgctcgagct gcgcgtacaa cgcgcgcatc
     7381 gcgggcgact tgccgacgag ctcaccgtac gattcgcgcg aggacaacgg cagctcggcg
     7441 aacgaatcgg caagctggat ctcgacctcg gtgcggccga gaaggagtgt ggccgtgcct
     7501 tcgagatgcg cctcgacgat cgagagtccg ttgatgcgcg taccgttctt gctgccgagg
     7561 tcgcgcacgg tgatcgcgcc gtcgcccatc gtgagctcgc aatgaaagcg cgacaccgtc
     7621 gcatcggtca gcgcgagctc tgcagacgcg tgcgtgccga ttacgactcg ctgcccggtc
     7681 gacacgacgc gcgctcctgc gttcgggccg gcgacgacgg tcaacacgaa gcggcggatc
     7741 gcgagcgcgt tttcgcgcac caccgccgcc gtggtcactg gatgctcgtc gcccgccatt
     7801 cggcggatgc tacacgcatc gatcgcgacg cccgtgaacc tccacgccgc acgcacatgg
     7861 ggcaccaggt gtcggagcta acgctcagaa agctcgttgc gctcgacgcg acgagtcagc
     7921 gtcgctgagc tcgatatagc gcgtaaaatc aacgtactct ggattagtag tctgcagtgg
     7981 tggaaggtgt gatatcgaaa aaagaggtca tgcctcgtac gctgaaggag accaacgaga
     8041 cgctcgtgat cgagcttccc gttagcggcc caccgccggt gccgcagcct tcgttaagac
     8101 cagcgggcac gttgcccaag ccgccgcgcc gactcgcggc cggcacgata ccgccagtat
     8161 cgcgctccaa gcgacgtgcc ccgaaagagc tgggcatcgg ctcgcgaatc ggaccgtggt
     8221 tgatcgagcg cgagctcggg cgtggtggga tgggccgggt ctacgcggtc gcgcacagtg
     8281 ggtttggaaa gcgtgccgcg ctcaaggtgt gtcacacgtc gatgatcgat cgcgacctcg
     8341 ctgcgaacac gttcttgcgt gaagcgcgca tcgtgaacct gatcgatcac cccgccgtgc
     8401 ccgacgtgtt cgcgaccggc acgttcgaga atcgcccgta cctcgcgatg gagcgtctcc
     8461 agggcgagac cctacgccag ctgtggtcgt cgggtgcgct gtcgcaagaa tccgcgctcg
     8521 agatcctcgt cgagctctgc ggcgtccttc aggcggcgca cgacgcaggt gtcgtgcatc
     8581 gcgatctgac gctcgacaac gtctttgtgc tcgacaaacc cggcgcgggt ggctgtcgca
     8641 tcaagctgct cgactgggga ttcgcgcgcg tactcgccga ggacgatccg ctgcgcggcc
     8701 acatcgccgg cacgatgacg tacgtcgctc ccgaagcgat cgtcgggcgc gaggtttcgc
     8761 ctgcaagcga tgtgtatgcg ctcggcgtcc tcgcctatat cctgcttctc ggtcaggcac
     8821 cgtttgcggc cgacgagctc gtcgagctcg cggcgatgca tctccaccaa gagccgcccg
     8881 ccccccaacg cctgcgcccg gcgatccgtc cggagctcga acagctcttg cttgcgatgc
     8941 tagccaaaga gccatcctgg cggccggttc ttgcggagat ccagcgcgag ctgagtgcgc
     9001 ggccccgccg tcgccgccgt tggcttcaac tcgtgccgtg aggcagagct tccgcgaagc
     9061 gtactcctgg caaagcttct gcgaagcgcg ctactgccgt gaggcaacgc ttccgcgaag
     9121 cgcactcgcg ttcgtgggcg ccgtgataat ctagctgcgt cgtacgcggt gtgaggcgcg
     9181 ggtgttgcgg cgggttggcg gatcgtgcgc tagcgacacg gcggcggcgc acgaagtcgc
     9241 atcgatgaac ctcgccgacg atcaatttcg cctcctggtc gagtccgtcc aggactacgc
     9301 catctatttg ctcgatcctt cggggctcgt tcgatcatgg aacgcaggtg ccgaacggct
     9361 caaaggctat acagccgagg aggccatcgg caggtcactg gcgacgttct tcatacccga
     9421 cgaccgcgcg agcggaaagc cgcagcagtt gctagcggcg gcactcaagt tcggacgcta
     9481 cgaggatagt ggctggcgag ttcgcaaaga cggcagtcag ttctgggcga atgcggtcat
     9541 cactgcgctg ttcgaagacg gtcgcgcggc cccggcgccg ctgtcgccga cagcgcttgg
     9601 cggcattcgc atagttctcg tcgacgacga cgaagatacg cgacaggcga tcgcggatgc
     9661 cctcgcgtcc gtcggcgcga gtgtggagca agctgtttcc gcatcggatg cggtgcagcg
     9721 gctgcagctc aatcggcccg acgtactcct gagcgatatc ggtatgcccc tggaagatgg
     9781 atacgctctg attcggcgcg tgcgctcgct ggcgcccgac ctcggtggtg acgttccggc
     9841 gatcgcgttg accgcatatg cgcggcacga agacgcgacg aaggctgaag catcgggctt
     9901 ccaactgcat gtctcgaaac ctgtcgcact cgaagacttg atcgacgcca tcaggagctg
     9961 tctgccgtct cgttccggaa ccgcgcggta acctcgacgc gctgggcgac ggcacccggc
    10021 tgggacgtac actctgccgg cgccgcaatg ataggttcgc cgggatgcgg acatcgctcg
    10081 tcgtgatcgt gctcctcctc gcgtgttcga agtcctcgag cgaacaacag gcgggctcgg
    10141 cagcgccacc tgctgcgagc ggctcgagtg gatcgagcgc cgcgccggct ggctcgggat
    10201 ccagcgctgc gcccgtcgag gctgcgacgg tggtcggtcc cactcgttcc gtcagcggag
    10261 agctggacgt gagcgggttg atgtccggca agtttcaatg gatcaagaag gaccagacgt
    10321 cgccgatcag ttgcgcgtgg agcgtcgaga aggagatcgg aggtcttcac gtcgatctga
    10381 gtgacggggc agggcacctc ctcaagatga cgatcgatgt tccgccgacg gaggcgggcg
    10441 tgccgcgact cgacgtcacg tcgaccggac ttccggcgcc actcaaggcg aagctcggct
    10501 tcaatatgag cggcgaagag accggcaagt tcgcggtgaa gttcgacacg acgctcaccg
    10561 agaatcccga tgcagagccc gcgaagaagc caggcaagaa ggacgagaag ccgagcggac
    10621 catcactgac gatcaagggc acgctcgagg tcacctgtcc gccgaagaag tgatcacggc
    10681 gcccgcagtg ccgctcgtgc tatagcgact tcgtgccgaa cgatccggac agccacggct
    10741 cgcccgacgc tgcgaaggtt cggctgatct gcagcatgtg ctcgcagccg tacttcgcga
    10801 cggccggcac gaaccccggt gagtgcgtgt cgtgcatgct cggccgcgcc gaagctgagg
    10861 cgcgccaagc agctcacgag cgcaagcagg cggggcgcag ggccctcgca agccgaatca
    10921 cgaaccgggt cggtgcgcgc ctgatcgtcg gcgtgatcgt gctcgcaatc ggtggcgtgc
    10981 tcgttgcgtg gcaggcggtc cagcatgcgc gcgagaaggc ggccgaacgc caccgcgcga
    11041 acgcgagtgc caacgtctac gtgatcaaga ccgagaattg gaaatggcgc atgtgtcact
    11101 gcgaggactt cgcgtgcgcg gcgcaggtca aagccgactt cgaagcgtgg acagcgaagg
    11161 aggccgcgcc cgacgatgca gacgtgaagc gggcggcaaa gagcgcgacc gattcgctgc
    11221 tccactgcta cgaccagctg cgctgaacgg cttcgtcggc tattcgagga tcgcgctctg
    11281 gaccttctcg a
//
LOCUS       DASUFX010000841        13550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1805181, whole genome shotgun sequence.
ACCESSION   DASUFX010000841 DASUFX010000000
VERSION     DASUFX010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805181"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFV99_33795"
     CDS             <1..961
                     /locus_tag="VFV99_33795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064414.1"
                     /translation="NALFLVHPRAERWSVSDRQRRRIIDNALALVSQPLTRNRTRVMA
                     RHALLHNLFHLKRNDIVVSWWTGRARFQGQKPSARLTAWPSIRRVREDVTVVDFDDLL
                     AVPDTAPIIATLLRRTPLTQLLDSHAGAPPLHWEDAVFLLRDHELARAIAYRLVPDVG
                     PRDQVAGPARLAAAFEQMLERAPDEMDVRAVAAFLVHLSALFALGEIHLREVNAKSPL
                     ISTVLAAEAAGQRPRGLTTLFALPGALATVDPRLATPPGIESIPALAARWRVHRAQTA
                     ELLSEPVIDALAQRLRRHLRGERRDPSEVVSLPSAAPPQPPST"
     gene            1026..1844
                     /locus_tag="VFV99_33800"
     CDS             1026..1844
                     /locus_tag="VFV99_33800"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5064415.1"
                     /translation="MNRGKPVTGWRRVASAMWRSPDDPQIFGALEVDARPAQHFIKHA
                     RAAGHHVTPTHLVGRALAHALVAVPDLNVQLRRGRVVPRPSVDIFFVTSVAGGRDLSG
                     IKIVDVPSRPAIEVASELHDRATAMKAGRDRDFAMTKRFMDRTPPWLLRPALRAATFV
                     TDQLGIDLPMLALRKFPFGSAMVTSVGMFGLPSGFAPLAWMYDVPVLLLVGEIAMKPV
                     VVDGRVEPMPTLPITATIDHRYVDGWHVSKAMAAFRDYLAAPDRFEPVVGLHAA"
     gene            1899..3128
                     /locus_tag="VFV99_33805"
     CDS             1899..3128
                     /locus_tag="VFV99_33805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5064416.1"
                     /translation="MVAPPTTPRSFAVDVARVSAAFQDLARQMNAQFLDKQEIIRLMT
                     ISAIAGEHMVIVGPPGTAKSAMIDMFAKLIDARYFEYLLTRFTEPNELFGPVDISAFR
                     EGKYTRRLDNMLPTAEIVFLDEIFKSNSAILNSLLHVINERKFQNGPEVIPVPLISLY
                     AASNEVPNDENLAAMFDRFLVRVLSDNLDSYHFHELMKKGVSLEVRKMTGRGMDMTGR
                     PAPDLRPVTSARELRSIQQNFDKFMVFPEEFLTKYKGLVFQIRSEGISVSDRRAVKLL
                     KLFAASAVFDGRTRVHDGDFFVLRHIWNNLDQVELLEEIINPVVDGYYREHPDERRFI
                     GPSASLDDLLTELGLIRELLTSGTELSDIQLFSQLKNLNEIKVALAALPGDAAARMIR
                     EVDQLLETVFASSKFGL"
     gene            3292..3669
                     /locus_tag="VFV99_33810"
     CDS             3292..3669
                     /locus_tag="VFV99_33810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX5064417.1"
                     /translation="MARARSASPCGHRRGHRGASTTGPGFLEHVYEAAMTIELTARRI
                     PFQRQVTFPIYYKDVLVAESRLDLLVAEQLVVELKAVEELRPIHTAQVLSYLRTGEFQ
                     LGLLFNFNVYSLRQAIQRIVWTG"
     gene            complement(3776..5050)
                     /locus_tag="VFV99_33815"
     CDS             complement(3776..5050)
                     /locus_tag="VFV99_33815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064418.1"
                     /translation="MRVAVALALLTGVTACRSSGDGGGSRTITVNAFAGDQPEGGATV
                     IGHAPDGSVIDQTTADAVGQATISVDADSLVSVIFPGNIGALTPVISVVTVAAEADTL
                     SIYGPRYGGPPPLIVGALQVDAPNLNAADYFDIHIGCATTRVQQLPATIDVGACSMGS
                     DTKLDVLLTGSHDVGSDPPAPQLDGYAAARVDMVNGLATFTAPAWQTTGPSVPVTLDG
                     VMPLVMVEPLSDGLSFGAQQATDHATLWTGLVVDATRITASLAGINAARVTTREVAGA
                     PTAINFGTSDFLPPIDVTAALTLSTTTLTWDPNSIGDTVNMHATWDVDSSVAAAVPTG
                     PHRVIWDAVLPPDATDVTLPVLAGDLGVAINPKDIAPVDIVVRYIDADGADGFAGLLA
                     AGVHAEDTLQASTIAPRPTDGEIRVSHAIGLR"
     gene            5125..6645
                     /locus_tag="VFV99_33820"
     CDS             5125..6645
                     /locus_tag="VFV99_33820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064419.1"
                     /translation="MPIPAERRRSYLARQVATMVLSHVQTDTPMAIVRASAWSNSLAR
                     MGVHLPLFLVHDIGVMLSLTRGAGGYVLRPRESQLARVQLPANVKPQLDQYKRLLENI
                     AQSEVIERLAGLRLRDEMVAVLLSRILGDTFNRWRDRSKSAGVEALPLDLGILGEIDF
                     ADHFRDFDPRSLWGFVEHLVAQSMHIYTQVELIDLDTVRLLGLFKEDSASGSEALGGA
                     IDLVDLFAALGSPEANDVANFSLDLLPSVLETRRSTGAQTFAVDGYASIERKGNIDSL
                     VLSELAYDREIFEQKVVDNELLYYGRDKQREDERRLQYLLIDCSASMRGQRQVFARGL
                     ALALIKKLTLEGDEIWVRFFDSRLHETVKIGRSGQVPVPYLLSFRSERGRNYGKVFRQ
                     LLVEVTRLRREQKRRVVMYVITHGQCHIEPELVTTLKQQAFLYGIVCLPSSDQLPDFV
                     PLLDRSQIVSADALTSRAERQRRALDIVGDASAAPKGPKPTTTAPSASMRNMPAVK"
     gene            6645..8456
                     /locus_tag="VFV99_33825"
     CDS             6645..8456
                     /locus_tag="VFV99_33825"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5064420.1"
                     /translation="MARRTFNSIESTADQAEAFAQQGRWADAERHYRELIGQTHVINY
                     EYDDWLRRLGDIYKNLGRQREAAFVYLYLHYFDMARAMLVGEQHVGLRARLAEIEKKW
                     SEAATLYQQAKLPVHSAVSFERAKQYTDATAAWRQLLHHPELGHRPYEAALVHFNYGL
                     AAVRLDPHSGEARRALIESQRKLEQVADDFEQVGELERAFDCFQILLKLGKESAQFEN
                     LAEGYVNCIRVLREDNLKFYVLQYYEDFIKLALERGELHAAATLYQEAAAFAARAGLP
                     YDRHYQHKSALTWMKCADKFVETGVPVQMIENALLAAASQHSAVGDYPSVRECFERLA
                     GLELTDRAKKRFSSIALRYKGLAAPPVELPGLPDYLKQQHAYADIWFVDLLEWEMDGD
                     AFAVAASIVGDLKYPNGIRRRALVVLLNLSDAERRNAQGETETLVDVAEKLGELQSYA
                     ALSPLEKLYASQDATIRRAAVRALRFLYFKRSFVIVRKALADPNDQVREAALMAIGGL
                     HFPHAFNPLARIYRESTDPRVRAAALQSIGKIQTVEAGEFLVMVLRQEGGNLREAATQ
                     ALAQMDNADVLPILRQHHEIETNPQVRDTIGELLRRH"
     gene            8511..9065
                     /locus_tag="VFV99_33830"
     CDS             8511..9065
                     /locus_tag="VFV99_33830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064421.1"
                     /translation="MGHYTSAVDLPRPRVDLELGRRRHRRITGPSGLLLFVCLFLPAV
                     KSCGEPVYPLAMPMFWHPYIYGLVLALATVRGTVRHVHYTVIALRILAWLTVVGAALL
                     GLFAAGVALVELFVGALLLAAVGRRGTSERRVAVTTVLVGALSLLWFGLWGITPDAML
                     GVYLSVGASAGLLVGGLVWLVETS"
     gene            9083..10018
                     /locus_tag="VFV99_33835"
     CDS             9083..10018
                     /locus_tag="VFV99_33835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064422.1"
                     /translation="MGGALFRSAFTLAVVAAFAGCDSRAKASDPQGGRAESKSKEYES
                     CGASMHCQDDLRCFDNVCRRTARSAVGDYFAALGTQLRAKGELNAAVDAFNRALGHYD
                     SEKIALPPDVDCAYGSALAAARQKKETAELAARVLHRCVLAVPSGSSLRERALVDLAL
                     LNESGLDPLALGRTQLADVYLTRAPQAPSSDKLQVSVTASPPVDKKTYQLIPDALGGP
                     DIKAALVACWESYNQATHKDALSSTISIKISYQASEYEDEPGKFTTKIDPAVAMPAGP
                     EASADQCVRAAVEPAIKNITGLRDAFATKLTITIK"
     gene            complement(10043..10408)
                     /locus_tag="VFV99_33840"
     CDS             complement(10043..10408)
                     /locus_tag="VFV99_33840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX5064423.1"
                     /translation="MRNREVIEREMYRAREDLEANLSELKHVVQEKVDVKARARVAVE
                     KGKLMAADAFDRSKQTAREYALRGKDGAVRLAKAGNDKVYFTYLRAKDRPVLTSSIIV
                     GVVATGVLTYYVGRRRCWW"
     gene            complement(10401..10835)
                     /locus_tag="VFV99_33845"
     CDS             complement(10401..10835)
                     /locus_tag="VFV99_33845"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5064424.1"
                     /translation="MDKTQSIAGESGNGRVSDLLHRITDDLKVIARDEVELVKGEFNH
                     TAKTTAFEASIIILGGIVALIGLGMLCAAAVAALAPVIHSLALRLLLMAAVYLVIGGA
                     VAASFAKRLSKDVRPDTKVLGYEAQRTLKGVKSALTEEGLHA"
     gene            complement(10846..11016)
                     /locus_tag="VFV99_33850"
     CDS             complement(10846..11016)
                     /locus_tag="VFV99_33850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064425.1"
                     /translation="MKKLAWTALVTAVSTAAARLAVRALVRMWRQVTKENPPPTSRWA
                     QFLVGKTTKAVT"
     gene            11102..12202
                     /gene="rlmN"
                     /locus_tag="VFV99_33855"
     CDS             11102..12202
                     /gene="rlmN"
                     /locus_tag="VFV99_33855"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5064426.1"
                     /translation="MEKPDLRAMTLADAEAFAVEQGWRRFRGEQVWRWVHEKGARSFD
                     EMSNLPVDVRAELAERAQIGTLTIAEVQTSQDGTRKLRLLTRDGQSIESVIIPNGDKT
                     TQCISSQVGCAVDCQFCATAKLGLKRNLDAGEIVDQVYLGRRLLAEVDPGRRLTNLVY
                     MGMGEPLHNYDNMVKSLRILTHDKGAGLSQRRITVSTAGLVPKLEKLGAEDVRPNLAV
                     SLNAPNDEIRDEIMPINRKWNIGKLLGALREYPLEQRRRITFEYVLLAGVNDSLRDAA
                     QLSKLLRDLKCKVNIIPWNPHPEAPYQRPSPAVIDAFQNECKRLGLLTYLRMPRGDDI
                     DAACGQLANRSNGEAIVPLRIRKSPPPPPPVE"
     gene            12209..12565
                     /locus_tag="VFV99_33860"
     CDS             12209..12565
                     /locus_tag="VFV99_33860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064427.1"
                     /translation="MSDERTKAGFWHGVFAGFCGFIMVVQLAIASQLSDVASMYRDFG
                     DVKLPVLTRLTIHPAWTWGTSLLGIGAVAALVVKRPRSIALYVATAVVLLAVAIATYW
                     YPRAPIYALAGNISAD"
     gene            12584..>13550
                     /locus_tag="VFV99_33865"
     CDS             12584..>13550
                     /locus_tag="VFV99_33865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064428.1"
                     /translation="MTSGAQPSRKLLIAAGALTQVIGTACLVLALASLPVAHDLRTGM
                     GGVFASIVAALAAIVCGTLVWRGRLVPLALAAGLDVGFGIGLPRGGSAIGAIVRMLPA
                     EDVSTAETLIVVAAIIMFAAAALCIIAVPSALKLRQWARAELAKDDPTASREFPLGTD
                     PAAQPLDEVAPTKERPRLERPSAGNTLRGFGTVKLMPTQVIRVDGPGRGKPIVIVGVS
                     VTLIAIGIIVITAATGGSDTSVSAAPGSGSAVAVADARVGSASSTTALVEDAAVADAA
                     PLGESIDEFVVRFHAALAKATAADLALLFDANAFAFGVEAHDLAEG"
BASE COUNT         2214 a         4580 c         4362 g         2394 t
ORIGIN      
        1 caacgcgttg ttcctcgtcc acccgcgtgc cgagcgctgg tccgtcagcg atcgccagcg
       61 ccgtcgcatc atcgacaacg cgcttgcgct cgtgagccag ccgctgacgc gcaaccgcac
      121 gcgcgtgatg gcgcgccatg cactgctcca caacctgttt cacctcaagc gcaacgacat
      181 cgtcgtgtcg tggtggaccg ggcgcgcgcg ctttcagggc cagaagccgt cggcgcgtct
      241 caccgcgtgg ccgagcattc gccgcgtgcg cgaggacgtc actgtcgtcg atttcgacga
      301 cttgctcgcc gtccccgata cggcgccgat catcgcgacg ctgctgcgcc gcacgccgct
      361 gacccagctg ctcgacagtc acgccggcgc accgccgctg cactgggaag acgcggtgtt
      421 cctgcttcgc gaccacgagc tcgcgcgtgc gatcgcgtat cgcctcgttc ccgacgtcgg
      481 tccgcgcgat caggtcgccg gccccgcacg cctcgccgct gcattcgaac agatgctcga
      541 gcgcgcgccc gacgagatgg acgtccgcgc ggtcgccgca ttcctcgtac acctctccgc
      601 attgttcgcg ctcggtgaga tccacctgcg cgaggtcaat gcgaagagtc cgttgatctc
      661 gaccgtgctc gcggccgagg ctgccggcca gcgtccacgc ggactcacga cgctgttcgc
      721 gctgcccggc gcgctcgcta ccgtcgatcc acgcctcgcg accccgcctg gcatcgagtc
      781 gatcccggcg cttgcggcac gctggcgggt tcatcgtgcg cagaccgcgg agctgctcag
      841 cgagccggtc atcgacgcgc tcgcgcagcg actgcggcgg catctcagag gcgagcgacg
      901 cgatccgagc gaagtggttt cgctaccatc ggccgcacca ccgcagccgc cgtcgaccta
      961 gctcgcgcag gcgttgcgca aaaactcgct cgtggcctgg cgagcggcat gcattacgta
     1021 ccggtatgaa tcgaggcaaa ccggtgaccg gctggcggcg tgtcgcaagt gcgatgtggc
     1081 gttccccgga cgacccccag attttcggag cgctcgaggt cgacgcgcga ccggcgcagc
     1141 atttcatcaa gcacgcgcgc gccgcggggc atcacgtcac gccgacgcat ctcgtcggtc
     1201 gggcgcttgc tcacgcgctc gtcgcggtgc ccgatctcaa cgtgcagctg cggcgtggtc
     1261 gtgtcgtgcc gcggccgagc gtcgacatct tcttcgtcac gtcggttgcg ggcggtcgcg
     1321 atctgtcagg gatcaagatc gtggacgtcc cgagtcgccc ggcgatcgag gtcgcaagcg
     1381 agctgcatga tcgcgccacc gcgatgaaag ccggccgcga tcgcgacttc gcgatgacca
     1441 agcgcttcat ggatcgcacg ccgccatggc tgcttcgccc cgcgctgcgt gccgcgacgt
     1501 tcgtgaccga ccagctcggc atcgacctgc cgatgctcgc cttgcgcaag ttcccgttcg
     1561 gcagtgcgat ggtgacgagc gtgggcatgt tcgggttgcc cagtggcttt gccccgctcg
     1621 cctggatgta cgacgtgccc gtgctcttgc tcgtcggcga gatcgcgatg aagccggtcg
     1681 tggtcgatgg ccgcgtcgag ccgatgccga cgctgccgat caccgcgacc atcgatcatc
     1741 gctatgtcga cggctggcac gtcagcaagg caatggccgc gttccgcgac tatctcgccg
     1801 ctccagaccg gttcgagccg gtggtcgggc ttcacgcagc ctaaacggcc ctgtgtcgct
     1861 tggatcgctg cgattttcgg gtaatgtgac ctttccgaat ggttgccccg cccaccacac
     1921 cgcgcagctt tgccgtcgat gtcgcgcgcg tcagtgcagc attccaggac ctcgcgcggc
     1981 agatgaacgc gcagttcctc gacaagcagg aaatcattcg cttgatgacc atctcggcaa
     2041 tcgccggaga gcacatggtc attgtcggtc cgcccggtac tgcgaagtcg gcgatgatcg
     2101 acatgttcgc gaagctgatc gacgcgcgtt acttcgaata cttgctcacg cgcttcaccg
     2161 agccgaacga gctgttcgga ccggtcgaca tctcggcatt ccgcgaaggc aagtacacgc
     2221 gccggctcga caacatgctg ccgacggcag agatcgtctt cctcgacgaa atcttcaagt
     2281 cgaactccgc gatcttgaac tcgctgctcc acgtgatcaa cgagcgcaag ttccagaacg
     2341 gccccgaggt catcccggtg ccgctgatct cgctctacgc ggcgtcgaac gaggtaccga
     2401 acgacgagaa tctggcggcg atgttcgacc gcttcctcgt ccgcgtcctc tccgacaacc
     2461 tcgacagcta tcacttccac gagctgatga agaagggcgt ctcgctcgag gtccgcaaga
     2521 tgaccggccg aggcatggac atgaccggtc gcccggctcc cgatctgcgc cccgtgacga
     2581 gcgcgcgcga gctgcggtcg atccagcaga acttcgacaa gttcatggtg ttccccgagg
     2641 agtttctgac caagtacaag ggactcgtct tccagatccg cagcgaaggc atctcggtct
     2701 ccgaccgccg cgcggtcaag ctgctcaagc tgttcgcggc gtcggcagtg ttcgacggcc
     2761 gcacgcgcgt ccatgacggc gacttcttcg tgctgcgcca catctggaac aacctcgacc
     2821 aggtcgagct gctcgaagag atcatcaatc cggtcgtcga cggctactac cgcgagcatc
     2881 cagacgagcg ccgcttcatc ggtccatcgg cgagcctcga cgatctgctc accgagctcg
     2941 gcctgattcg cgagctgctc acgagcggca ccgagctctc cgacatccag ctgttcagcc
     3001 agctcaagaa cctcaacgag atcaaggtcg cgctcgctgc gctgccaggc gacgctgccg
     3061 cgcgcatgat ccgcgaggtc gatcagttgc tcgagaccgt gttcgcgtcg tcgaaattcg
     3121 ggttgtaggc gagaaccgcg ccgcagtgaa ttcgacgggt taatccgtcg ccgatcgccg
     3181 gcgtgcgcat cagcaaccga tccactggct ggccgaaagc ggccctcaga tggaacgcca
     3241 agatgccaag aacgccaaga acgccaaggt aagaaacctt cgccgggagc catcgcccga
     3301 gctcgatccg ctagcccatg cggtcatcgg cgcggccatc gaggtgcatc gacaacgggc
     3361 cccgggttcc tcgagcacgt ctacgaagcg gcgatgacca tcgagctcac ggcgcggcgg
     3421 atcccgtttc agcggcaggt gacgttcccg atctactaca aggacgtcct cgtcgccgag
     3481 tcgcgcctcg atctcctcgt cgcggagcag cttgttgtcg agctgaaggc ggtcgaagag
     3541 ctgcgaccta ttcacacggc gcaggtgctc tcgtatctgc ggacgggcga gtttcagctc
     3601 gggctgttgt tcaacttcaa cgtgtactcg ttgcgtcagg cgatccagag gattgtctgg
     3661 accggataga tcttcttctt ggcgttcttg gcggccttgg catcttggcg ttccatctgg
     3721 ggagcggcgc tcatgcgcgc caaatagaac taggcacggc attcggtcgc acgagtcagc
     3781 gcaggccgat cgcgtgactt acgcggattt ctccgtcggt gggacgcggt gcaatggtgc
     3841 tggcttgcag cgtgtcctcg gcgtgcacgc cagcggcgag cagtccggcg aagccgtccg
     3901 cgccgtccgc gtcgatgtag cggacgacga tgtcgaccgg tgcgatgtct ttcgggttga
     3961 tcgcgacgcc gaggtcaccg gccagcacgg gcaacgtcac gtcggtcgca tcgggtggca
     4021 acacggcatc ccagatcacg cgatgcgggc cggtcggcac tgccgcggcg acactgctat
     4081 cgacatccca cgtcgcgtgc atgttgaccg tgtcgccgat cgaatttggg tcccacgtga
     4141 gcgtcgtggt cgacaacgtg agcgctgcgg tgacatcgat cggcggcaag aagtcgctcg
     4201 tgccgaagtt gattgcggtt ggcgcgccgg cgacttcgcg cgtcgtcacg cgcgctgcgt
     4261 tgatgccggc aagcgatgcc gtgatccgcg tggcgtcgac gacgagccca gtccacagcg
     4321 tggcgtggtc ggtcgcctgc tgcgcgccga acgacagccc atcggacagc ggctcgacca
     4381 tcacgagcgg catcacgccg tcgagcgtga cgggcacgct cggccccgtc gtctgccacg
     4441 cgggcgcggt gaacgtcgcg aggccgttga ccatgtcgac acgcgctgcc gcgtagccgt
     4501 cgagctgggg cgcgggtgga tcgctgccga cgtcgtgaga accggtgagc agcacgtcga
     4561 gcttggtatc gctgcccatg ctgcacgcgc cgacgtcgat cgtcgccggc aactgctgca
     4621 cgcgcgtcgt cgcgcagccg atgtgaatgt cgaagtagtc ggcggcgttc aagttcgggg
     4681 cgtcgacctg caacgcaccg acaatcagcg gcggcggtcc gccataccgc gggccgtaga
     4741 tcgacagcgt gtcggcctcg gcggctacgg tcaccacgga gatcaccggt gtcagcgcgc
     4801 cgatgttgcc tggaaagatc accgagacca gcgaatcggc gtcgacgctg atcgtcgcct
     4861 gaccgaccgc atcggccgtc gtctgatcga tgaccgagcc atccggtgcg tggccgatca
     4921 cggttgcgcc gccctccggc tggtcgcccg cgaatgcatt gacggtgatc gtgcgcgagc
     4981 caccaccgtc tccggacgag cgacaggcgg tgacacctgt gagcagcgca agcgcgacgg
     5041 cgaccctcac ccggctatcg taccgcgcaa ggtatcggcg cgccgcgaat cccgtcacgc
     5101 tcggatttgg gctagcttgt agccgtgccg attcccgccg agcgcaggcg cagctacctg
     5161 gcccgccagg tcgcgaccat ggtgctgtcg cacgtgcaga ccgatacccc gatggcgatt
     5221 gttcgcgcct cggcgtggtc gaattcgctg gcgcggatgg gcgtgcacct gccgctgttc
     5281 ttggtccacg acatcggcgt gatgctgtcg ctgacacgcg gcgccggtgg ctacgtgctg
     5341 cggccgcgcg agagccagct cgcccgcgtg cagctcccgg cgaacgtcaa gccgcagctc
     5401 gatcagtaca agcggctgct cgagaacatc gcgcagtccg aggtcatcga gcggctcgcc
     5461 ggtttgcgtc tgcgcgacga gatggtcgcc gttctgctgt cgcgcatcct cggcgacacg
     5521 ttcaaccgct ggcgcgatcg cagcaagagt gccggtgtcg aggcgctgcc gctcgacctc
     5581 ggcatcctcg gcgagatcga cttcgccgat cacttccgcg atttcgatcc acgcagcctg
     5641 tggggctttg tcgagcacct ggtcgcgcag tcgatgcaca tctatacgca ggtcgagctc
     5701 atcgatctcg acaccgtgcg tctgctcggc ctgttcaagg aagacagtgc gtcgggtagc
     5761 gaggcgctcg gcggcgcgat cgatctcgtc gacttgtttg ccgcgctcgg ctcgcccgag
     5821 gcaaacgacg tcgcgaactt ctcgctcgac ctgttgccga gcgtgctcga gacgcggcgc
     5881 tcgaccggcg cacagacgtt tgccgtcgac gggtacgcgt cgatcgagcg caaaggcaac
     5941 atcgattcgc tcgtgctttc cgagctcgcg tacgaccgcg agatcttcga gcagaaggtc
     6001 gtcgacaacg agctgctcta ctacggccgc gacaagcagc gcgaagacga gcgccgcctg
     6061 cagtacctgt tgatcgattg ctccgcatcg atgcgcggcc agcgtcaggt gttcgcgcgc
     6121 ggtctcgcgc tcgcgctgat caagaagctc acactcgaag gcgacgagat ctgggtgcga
     6181 ttcttcgact cgcgcctgca cgagaccgtc aagatcggcc gttcggggca ggtacccgtg
     6241 ccgtatctgc tctcgttccg cagcgagcgc ggccgcaact acggcaaggt gtttcgccag
     6301 ctgctcgtcg aggtcacgcg cctgcgccgc gagcagaagc gacgcgtcgt gatgtacgtg
     6361 atcacgcacg gccagtgtca catcgaaccc gagctggtga cgacgctcaa gcagcaagcg
     6421 ttcctctacg gcatcgtgtg tttgccatcc tccgatcagc tgcccgactt cgtgccgctg
     6481 ctcgatcgct cgcagatcgt cagtgccgac gcactcacct cgcgcgccga acgccagcgc
     6541 cgtgcactcg acatcgtcgg tgatgcgagc gccgcgccga agggcccgaa gccgaccacc
     6601 accgcgccga gcgccagcat gcgcaacatg ccggcggtga agtaatggca cgtcgcacgt
     6661 tcaactcgat cgagagcaca gcggatcaag ccgaggcgtt tgcccagcag ggcaggtggg
     6721 ccgacgccga gcgccactac cgcgagctga tcggccagac ccacgtcatc aactacgagt
     6781 acgacgactg gctgcgccgg ctcggcgaca tctacaagaa cctcggtcgt cagcgcgaag
     6841 cagcattcgt ctacctctat ctgcactact tcgacatggc gcgcgccatg ctcgtcggtg
     6901 agcagcacgt cggccttcgc gctcgtctcg ccgagatcga gaagaagtgg tcggaggccg
     6961 cgacgctgta ccagcaagcg aagctgccgg tgcactccgc tgtctcgttc gagcgcgcga
     7021 agcagtacac cgatgcgacc gctgcctggc gtcagctgct ccatcatccc gagctcggtc
     7081 accgtccgta cgaggcggcg ctcgtccact tcaactacgg cctcgccgcg gtccgcctcg
     7141 atccgcactc cggtgaggcg cgccgcgcgc tgatcgagag ccagcgcaag ctcgagcaag
     7201 tcgccgatga cttcgagcaa gtcggcgagc tcgagcgcgc attcgactgc ttccagatcc
     7261 tgctcaagct cggcaaggaa tcggcgcagt tcgagaacct cgccgagggc tacgtcaact
     7321 gcatccgcgt gctgcgcgag gacaacctca agttctacgt gctgcagtac tacgaagact
     7381 tcatcaagct cgcgctcgag cgcggcgagc tacacgcagc ggcgacgctc taccaggagg
     7441 ctgcggcctt cgcggcacgc gccggtctgc cgtacgaccg ccattaccag cacaagagcg
     7501 cgctgacctg gatgaagtgc gcggacaagt tcgtcgagac cggcgtgccg gtgcagatga
     7561 tcgagaacgc gctgctcgcg gcggcgtcgc agcattcggc ggtcggcgat tacccgtcgg
     7621 tgcgcgaatg cttcgaacgg ctcgcaggcc tcgagctcac cgatcgcgcg aagaagcggt
     7681 tctcgtcgat cgcgctgcgc tacaaaggcc tcgcggcgcc gcccgtcgag ctgccgggct
     7741 tgcccgacta cctgaagcag cagcacgcct acgcggacat ctggttcgtc gatctgctcg
     7801 agtgggagat ggatggcgat gcgttcgccg tcgcggcgtc gatcgtaggc gacctcaagt
     7861 atccgaacgg catccgtcgc cgcgcgctcg tcgtgctgct caatctctcc gacgccgagc
     7921 gccgcaacgc gcagggcgag accgagacgc tcgtcgacgt cgccgagaag ctcggcgagc
     7981 tgcagtccta cgccgcgctg tctccgctcg agaagctcta cgcctcgcag gacgccacga
     8041 tccggcgcgc cgcggtccgc gcgctgcgct tcctctactt caagcgttcg ttcgtgatcg
     8101 tacgcaaggc gctcgccgat ccgaacgatc aagtccgcga agccgcgctg atggcgatcg
     8161 gcggtctcca cttcccgcat gcgttcaatc cgctcgcgcg catctatcgc gagagcacgg
     8221 atccacgcgt gcgagcggcg gcactgcaat cgatcggcaa gatccagacg gtcgaggccg
     8281 gcgagttcct cgtcatggtg ctccgccagg agggcggcaa cctgcgcgag gccgcgacgc
     8341 aagcgctcgc ccagatggac aacgccgacg tgctgccgat cctgcgccag caccacgaga
     8401 tcgaaacgaa cccgcaagtc cgcgacacca tcggcgagct gcttcgcagg cactagctcc
     8461 gaaatcctcg cgctcagcgc ggcgcggaag tcgtgtcatg ctcggcggcg atggggcatt
     8521 acacgagtgc ggtggacctg ccgcgcccgc gcgtcgacct cgagctaggg cgtcgccgcc
     8581 atcgccgcat caccggcccg tccggactgc tcctgttcgt gtgcttgttc ttgcccgcgg
     8641 tgaagagctg cggcgagccg gtctatccgc tcgcgatgcc gatgttctgg catccgtaca
     8701 tctacggcct cgtgctcgcg ctcgcgacgg tgcggggcac cgtgcgtcac gtccactaca
     8761 ccgtcattgc attacgcatc ctcgcctggc tcaccgtcgt gggagccgca ctgctcggct
     8821 tgttcgccgc tggcgtcgcg ctcgtcgagc tgttcgtcgg cgcgctcttg ctcgcggcgg
     8881 ttggccggcg cggcacgtcg gagcgacgag ttgccgtgac gaccgtcctg gtcggcgcgc
     8941 tgtcgctctt gtggttcggc ctctggggca tcacgcccga cgcgatgctc ggtgtgtatt
     9001 tgtcagtggg cgccagcgcc ggcctgctcg tcggtggcct ggtgtggctt gtcgagacga
     9061 gctgagtgct agtatcgggc gcatgggtgg agccttgttt cgttctgcat ttacccttgc
     9121 ggtagtcgcc gcattcgccg gttgtgattc gcgcgccaag gcgtccgatc cgcaaggcgg
     9181 tcgcgccgag agcaagtcga aagagtacga gagctgcggt gcgagcatgc actgccagga
     9241 cgacctgcgc tgcttcgaca acgtgtgtcg ccgcaccgcg cgctcggccg tgggtgacta
     9301 cttcgccgcg ctcggtacgc agctgcgtgc gaagggtgag ctgaacgccg ctgtcgacgc
     9361 gttcaaccgc gcactcggcc actacgacag cgagaaaatc gcgctgccgc ccgatgtcga
     9421 ctgcgcgtac ggttctgcgc ttgcggccgc gcgccagaag aaagagaccg ccgagctggc
     9481 cgcgcgggtg ttgcaccgct gcgtgctcgc cgtgccgtcg ggtagctcgc tacgcgagcg
     9541 cgcgctggtc gacctcgcgc tcttgaacga gtccggtctc gatccgcttg cactcggccg
     9601 cacccagctt gccgacgtct atctgacccg cgcgccgcaa gcgccgtcga gcgacaagct
     9661 ccaggtgtcg gtcacggcat cgccgcccgt cgacaagaag acctatcaac tgatccccga
     9721 cgcactcggc ggcccggaca tcaaggcagc gctcgtcgcg tgctgggaga gctacaacca
     9781 ggcgacgcac aaggacgcgc tgtcctcgac gatcagcatc aagatctcgt accaggcgtc
     9841 ggagtacgag gacgagcccg gcaagttcac cacgaagatc gacccggcgg tcgcgatgcc
     9901 ggctggcccg gaagcaagcg cggatcaatg cgtacgcgcc gcggtggaac cggcgataaa
     9961 gaacatcact ggtctgcgcg acgcgtttgc cacgaagctg acgattacga tcaagtgatc
    10021 gtcaccgacg atcgggtgat cgtcaccacc agcatctgcg gcgaccgacg tagtaggtga
    10081 gcacgccagt tgcgacgacg ccgacgatga tcgacgacgt cagcacaggg cggtccttcg
    10141 ccctgagata ggtgaaatac accttgtcgt tgccggcctt ggcgagccgc actgcgccgt
    10201 ccttgccacg cagcgcgtat tcgcgcgctg tctgtttgct gcggtcgaag gcgtcggcag
    10261 ccatcagctt gcccttctcg acagcgacac gtgcgcgagc tttgacgtcg accttctcct
    10321 ggacgacgtg cttgagctcg gacaggttcg cttcgagatc ctcgcgtgca cggtacatct
    10381 ctcgttcgat tacttcgcgg ttacgcatgg agaccctcct cggtcaacgc ggacttgacg
    10441 cccttgagcg ttcgttgcgc ctcgtagccc agcaccttgg tgtcgggccg aacgtccttg
    10501 ctgagtcgct tcgcgaagct cgctgccacc gcaccaccaa tcacgaggta gacggcggcc
    10561 atgagcaaca ggcgcagcgc gagcgaatga atcaccggtg cgagcgcggc gactgcggcg
    10621 gcgcacagca tgccgaggcc gatcaacgcg acgatgccgc caaggatgat gatcgatgct
    10681 tcgaacgcgg tggtcttcgc ggtgtggttg aactcgcctt tgacgagctc gacctcgtct
    10741 cttgcgatca ctttcaggtc gtcggtgatg cgatggagca ggtcactgac gcgaccgttc
    10801 ccgctttcgc cggcgatgga ttgggttttg tccatacttg atctcctacg tcactgcctt
    10861 cgtggttttc ccgacgagaa actgcgccca gcgcgaagtc ggcggcgggt tctctttcgt
    10921 gacctggcgc cacattcgta cgagtgcgcg caccgccaag cgtgcggcgg cggtcgaaac
    10981 ggctgtcacc aatgcggtcc aagccagctt cttcatacgc aatgtttcgt gcagtcgacg
    11041 tgccggtact gctgcgagaa atccctttgg caccgtgcgt tcgctgtagt acctagctgg
    11101 ggtggaaaag cccgacctgc gtgcgatgac gcttgccgac gcggaagcct tcgccgtcga
    11161 gcagggctgg cgacggtttc ggggcgaaca ggtctggcgc tgggtccacg aaaaaggcgc
    11221 gcgcagcttc gacgagatgt ccaacctgcc ggttgacgtg cgcgccgagc tcgcagagcg
    11281 tgcacagatc ggcacgctca cgatcgccga ggtccagacc tcacaggacg gcacgcgcaa
    11341 gctgcggctg ctcacccgcg acggtcagtc gatcgagagc gtgatcatcc ccaacggcga
    11401 caagaccacg cagtgcattt cgtcgcaggt cggctgcgcg gtcgactgcc agttctgtgc
    11461 gaccgccaag ctcggcctca agcgcaatct cgacgccggt gaaattgtcg accaggtcta
    11521 tctcgggcgc cggctacttg ccgaagtcga ccccggccgc cggctcacga atctcgtcta
    11581 catgggcatg ggcgagccgc tccacaacta cgacaacatg gtcaagtcgc tgcgcatcct
    11641 cacgcacgac aagggcgccg ggctctcgca gcgccgcatc acggtttcga ccgctggcct
    11701 ggtgccgaag ctcgagaagc tcggtgccga ggacgttcgc ccgaacctcg cggtctcgct
    11761 caacgcgccg aacgacgaga ttcgcgacga gatcatgccg atcaatcgca agtggaacat
    11821 cggcaagctg ctcggcgctc tgcgcgagta tccgctcgag cagcgccgcc gcatcacgtt
    11881 cgagtacgtg ctgctcgcgg gcgtgaacga ctcgttgcgc gacgccgctc agctctcgaa
    11941 gctgctgcgc gacctcaagt gcaaggtcaa catcattccg tggaacccgc atcccgaagc
    12001 gccgtatcag cggccgtcgc cggcggtgat cgacgcgttc cagaacgagt gcaagcggct
    12061 cggcctcttg acgtacctcc gcatgccgcg cggtgatgac atcgacgccg cgtgcggcca
    12121 gctcgcgaac cgcagcaacg gcgaggcgat cgtgccactg cggatccgca agtccccgcc
    12181 acctccgcct ccggtagagt agtcggcggt gagcgacgag aggacgaaag cgggcttctg
    12241 gcacggcgtg ttcgccgggt tctgcggctt catcatggtc gtgcagctcg cgattgcgag
    12301 ccagctctcc gatgttgcct cgatgtaccg cgacttcggt gacgtgaagt tgccggtgct
    12361 gacgcggctc acgatccatc cggcctggac gtggggcacg tcgttgctcg gcatcggcgc
    12421 agtcgctgcg ctcgtcgtca agcggccgcg ctcgatcgcg ctgtatgtcg ccaccgccgt
    12481 cgtgctgctc gccgtcgcga tcgcgacgta ctggtacccg cgcgcgccga tctacgcgct
    12541 cgccgggaac atctccgccg actgagtcgt acaatcgcct tggatgacgt cgggggcgca
    12601 gccatcgcga aagctgctga tcgcggcggg cgcgttgacg caggtgatcg gtacggcgtg
    12661 cctcgtgctc gcgctggcct cgctaccggt cgcccacgac ctgcgtactg gcatgggcgg
    12721 cgtgttcgca tcgatcgtcg cggcgctcgc ggcgatcgta tgcggcacgc tcgtctggcg
    12781 gggccggctc gtcccgctcg cgctcgcagc gggcctcgat gtcggtttcg gcatcggcct
    12841 gccgcgcggc ggctcggcga tcggcgcgat cgtgcgcatg ctgcccgccg aagacgtctc
    12901 cacggccgag acgctgatcg tcgtcgctgc gatcatcatg ttcgccgccg ccgcgctctg
    12961 catcatcgcg gtgccgagcg cactcaagct ccgtcagtgg gcccgcgccg agctcgccaa
    13021 agacgatccg acggcgtcgc gcgagtttcc gctcggcacc gatccagccg cgcagccgct
    13081 cgacgaggtc gcgccgacga aggagcggcc gcgcctcgag cgtccgagcg caggcaacac
    13141 gctgcgcggg ttcggaaccg tcaagctcat gccgacacag gtgatccgtg tcgatgggcc
    13201 cgggcgcggc aagccgatcg tgatcgtcgg cgtgtcggtg acgctgatcg cgatcggcat
    13261 catcgtgatc accgccgcga cgggtggctc ggacacgtcc gtcagcgccg ctccgggcag
    13321 tggctcggcg gtcgccgttg cagatgctcg cgtcggttcg gcgtcgtcga caaccgcact
    13381 cgtcgaggat gcagctgtcg ccgatgcggc accgctcggc gagtcgatcg acgaattcgt
    13441 cgtgcgcttc catgccgcac tcgccaaggc gacggccgcc gacctggcgc tgctgttcga
    13501 tgccaacgca ttcgcgttcg gtgtcgaagc gcacgatctt gcagaaggcc
//
LOCUS       DASUFX010000842         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_277612,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000842 DASUFX010000000
VERSION     DASUFX010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277612"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VFV99_33870"
     CDS             <1..1554
                     /locus_tag="VFV99_33870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064429.1"
                     /translation="KTPAGKTRVAVIYLNTLHAPEDKEFIVATIADRLYSWMLRNPNP
                     ELQALFYIDEVAPFIPPVEKPACKEDLELLFKQARKFGIGCLIATQNPGDVDYKALSQ
                     FGTWAIGRLATRQDLKKVEPALRSLAGADTDAVVEALPGLKPGELVVLSPDHFDAPCS
                     LATRRLVTAHQTLDEAMVERLAQARWRERFGVAPPPKQTSIAHDATLPAWAAPVEREP
                     AATSTKRPRAAPAKAAREARAAAATPTAPTPSTAADSQVTFDRVAAILGAKLAMTVDE
                     VARKAGLSLPKTRDALKALVAHDRAGTFRDGRTIKYWSRATGARPDLGMKARITAVQP
                     AIDRNGAEAIAQTLLRSKLLGLIGENEAFEQAQLEYRLVYRVMFEERVKKPLLARMVG
                     SSHEQRLGSVYLHARDLSVLEYSSDAGLRFAADLPAHASDVEDLDGIARFDEVRPADI
                     RFDEDEWRGRREAAEAKQHVRRLFGARPGVVEPVMVPLWKLILRRGAGESFRVVMIDA
                     LAGRIVDWP"
     gene            complement(1585..1764)
                     /locus_tag="VFV99_33875"
     CDS             complement(1585..1764)
                     /locus_tag="VFV99_33875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064430.1"
                     /translation="MRDPRTNPAIEGSCLCGAVHLAAARKPRTVTQCNCSVCRRYGTL
                     WAYYRRNAAVITAPR"
     gene            complement(1775..2161)
                     /locus_tag="VFV99_33880"
     CDS             complement(1775..2161)
                     /locus_tag="VFV99_33880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064431.1"
                     /translation="MNATARPTLATRLAELVMTQLDPCDLGDGIEVVHDVEALEIEME
                     CDVNDPAPVEPMPVEAAPVDDIASTFPFARQPADDVAATLTFARVDDVGTHEFVRFSA
                     PYERIEIQIDRNAFLRATIEEARRRG"
     gene            2274..3041
                     /locus_tag="VFV99_33885"
     CDS             2274..3041
                     /locus_tag="VFV99_33885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064432.1"
                     /translation="MSENSESSPVEPLKPAAPGRREPLELAARVMSIVMLGWLAAMAG
                     FAWMAYDDGYTSLVLPIWNSVFVLGMVPIVQGVFKRRLWGQRWVAGVSIFTGISNAWQ
                     ASRMDSTLLWFGALLLFAVAMVVRRARPLFNDSDGNRGRIQQLVATIVTVGSVVISLL
                     VLQGSGSERGRASFAREVQASYDAGAPGRVHVQVIDRGLVIESIDDTNEQIDAAADSL
                     QTQLSSVGPRAKAWAVGFEHIVITNGSYTRTLTPRSR"
     gene            3041..3406
                     /locus_tag="VFV99_33890"
     CDS             3041..3406
                     /locus_tag="VFV99_33890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5064433.1"
                     /translation="MSIDVKGSCHCGRVSLEVASAPTQVTSCNCSICRRLGTLWAYYA
                     PADVRVTGQTATYRRGDKTIDFHHCPSCGCTTHWSPVEGRDLNRMGINARLLAPEVFG
                     AARVRRLDGASDEWKYLDD"
     gene            complement(3413..>4906)
                     /locus_tag="VFV99_33895"
     CDS             complement(3413..>4906)
                     /locus_tag="VFV99_33895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064434.1"
                     /translation="STESNLDFDYLAFLQEVIDCAVVMVECDVALAAVDEKKRTRERE
                     IESQLTAVAEFGKRAAEFVGPVADQQAGSPAGRCAATIARAIRAAVESESAETKAKLA
                     AERDELAKNSQGINTRAKEALEKLLRTHDLPGAVKELEVVRSGTGMKATMRQRTSFAV
                     EAVLALEIPPTSLFTADLRVDRVAEGVEVHGREAGGWLKKSDKLVAHKLGRYQVVSVT
                     VGAIVCIQLRAEGNGGELIIRAAPTGDMSIESTGGSPAKEVSIEERDRAGLRSLVEKL
                     EAATRSLADARGNLVSIEIDGKPMADQANPRVLAERLTAAIGPTVQQINKHSRSDGEL
                     VLRRLIAGNRREEIFVPIADLVRKFEVLPAHARTVFSPLQLQGEPELKAPIVAKPVEA
                     KPVDVKPVEVKPVDAKSVEVKSAPMVPMPERTTSRTSPPPIVAATLPPRTKEPTAPQS
                     FAMGESKPPQPPPPAQVVDDDDDEWPSIARDEPSGAASDKGVPPPAK"
BASE COUNT          777 a         1586 c         1681 g          862 t
ORIGIN      
        1 aagacgcctg ccggcaagac gcgcgtggcg gtcatctacc tcaacacgct gcacgcaccg
       61 gaggacaagg agttcatcgt cgcgaccatc gcggatcggc tgtacagctg gatgctgcgc
      121 aatccgaatc ccgagctgca ggcgctgttc tacatcgacg aggttgcgcc gttcatcccg
      181 cccgtcgaga agccggcgtg caaagaggac ctggagctgc tgttcaagca agcgcgcaag
      241 ttcgggattg gctgtttgat cgcgacccag aatcccggcg acgtcgacta caaggcgctg
      301 tcgcagttcg gcacgtgggc gatcggccgg ctcgcgaccc gtcaggatct caagaaggtc
      361 gagcccgcgt tgcgctcgct cgccggtgcc gacaccgacg ccgtagtcga ggcgctgccc
      421 gggttgaagc cgggtgagct cgtggtgctg tcacccgatc acttcgatgc accgtgttcg
      481 ctcgcgactc gccggctcgt caccgcacat caaacgctcg acgaggcgat ggtcgagcgt
      541 ctcgcgcagg cgcgctggcg cgagcgattc ggagtggcgc cgccgccgaa gcaaacgtcg
      601 atcgcgcacg atgcgacgtt gcccgcatgg gccgcacccg tcgagcgcga gccggctgcg
      661 acgtcgacga agcgtccgcg cgcggcgccg gcgaaggccg cgcgtgaggc ccgcgccgca
      721 gcggcgactc cgacagcccc gacgccgtcg acagccgccg actcgcaagt gacgttcgat
      781 cgcgttgccg cgatcctcgg cgccaagctg gcgatgacgg tcgacgaggt cgcacgcaag
      841 gccggtctgt cgctgccgaa gacgcgcgac gcgctcaagg ctctcgtcgc gcacgatcgc
      901 gccggcacgt tccgcgacgg ccgcacgatc aaatactggt cgcgtgcgac gggcgcgcgg
      961 ccggatctcg gcatgaaggc gcggatcacc gcggtacagc cggcgatcga tcgcaacggc
     1021 gccgaagcga tcgcgcagac gctgctgcgt tcgaagctgc tcggcctcat cggcgagaac
     1081 gaggcattcg agcaagcgca gctcgagtat cgcctcgtgt accgcgtgat gttcgaagag
     1141 cgcgtcaaga agccgctgct cgcgcggatg gttggttcga gtcacgagca gcgtctgggc
     1201 agtgtgtacc tgcacgcgcg cgacctgtcg gtgctcgagt attcaagcga tgctggcttg
     1261 cggttcgcgg ccgaccttcc ggcgcacgcg agcgacgtcg aggacctcga cggcatcgcg
     1321 cgcttcgatg aagtccgccc ggccgacatc cgcttcgacg aggatgaatg gcgcgggcgc
     1381 cgcgaggcgg ccgaagcaaa gcagcacgtg cgtcggctgt tcggtgcgcg acctggtgtg
     1441 gtcgagccgg ttatggtccc gctgtggaag ctgatcctcc gccgcggcgc tggcgaatca
     1501 ttccgcgtcg tgatgatcga cgcgctagcc ggtcgcatcg tcgactggcc gtagctgatc
     1561 agcgggaaga tcgtttggcg tcggtcagcg cggcgccgtg atcacggccg cgttgcggcg
     1621 gtagtacgcc cacagcgtgc cgtagcgccg acacaccgaa cagttgcact gcgtgaccgt
     1681 gcgcggcttg cgcgccgctg cgaggtgaac ggcaccgcag aggcagctgc cctcgatggc
     1741 tggattcgtc cgcggatcac gcatgccgcg gatgttagcc gcggcgtcgc gcttcttcga
     1801 tcgtggcgcg caagaacgcg ttgcgatcga tctggatctc gattcgctcg tacggcgccg
     1861 agaacctgac gaactcgtgc gtgccgacat cgtcgacgcg cgcgaaggta agggtggccg
     1921 caacgtcgtc ggccggttgg cgtgcgaacg ggaaggtgct cgcgatgtca tcgacgggcg
     1981 cggcctcgac cggcattggc tcgaccggcg cggggtcgtt cacatcgcac tccatctcga
     2041 tctcgagcgc ttcgacatcg tggaccacct cgatgccgtc accgagatca catggatcga
     2101 gctgggtcat caccagctcg gcgaggcggg ttgccagagt agggcgggcg gtggcgttca
     2161 tcctgcaggg aggagccgcg gcgggtgtgc tggtcgcacg aaatccgaca gccacaaaaa
     2221 accgaccgcc gtgcgcgggc cgtggtgtat gccttcgcca cgaggaccgt cctatgagcg
     2281 aaaactctga atcatcaccg gtagaaccgc tgaagcctgc ggccccgggc cggcgcgagc
     2341 cgctcgagct cgcggcacgc gtcatgtcga tcgtcatgct cggctggctg gcggcgatgg
     2401 ccgggttcgc ctggatggcg tacgacgacg gctacacgag cctcgtgttg ccgatctgga
     2461 actcggtgtt cgtgctcggc atggttccga tcgttcaggg cgtgttcaag cgccggctgt
     2521 ggggccagcg ctgggttgct ggcgtctcga tcttcaccgg catcagcaac gcgtggcagg
     2581 cgtcgcgcat ggactcgacg ctgctctggt tcggcgcgct gctgttgttc gcggtcgcaa
     2641 tggtcgtccg gcgcgcgcgg ccgttgttca acgactccga cggcaatcgc ggcaggatcc
     2701 agcagctcgt cgcgacgatc gtgacggtcg gctcggtcgt catcagcttg ctggtgctgc
     2761 agggcagtgg ctcggagcgg ggccgcgcta gcttcgcgcg tgaggtgcaa gcgagctacg
     2821 acgcgggcgc accgggccgc gttcacgtgc aggtgatcga ccgcggcctc gtgatcgaga
     2881 gcatagacga cacgaacgaa cagatcgacg ctgcggcgga ctcgctacag actcagctga
     2941 gcagcgtcgg cccgcgcgcc aaggcgtggg cagtcggctt cgagcacatc gtgatcacga
     3001 acggcagcta cacgcgaacg ctaacgccga ggagtcggta gtgagcatcg acgtcaaagg
     3061 cagctgccat tgcggacgcg tgtcgctcga ggtcgcgagt gcaccaacgc aggtcacctc
     3121 gtgcaactgc tcgatctgcc ggcggctcgg cacgctgtgg gcgtattacg cgcccgccga
     3181 cgttcgcgtc accggacaga ctgcgacgta tcgccgtggc gacaagacca tcgacttcca
     3241 ccactgcccg agctgcggtt gcacgacgca ctggtctccg gttgagggcc gcgatttgaa
     3301 ccggatgggg atcaacgcgc gactgctagc gccggaggtg ttcggcgccg cgcgggtgcg
     3361 ccggctcgac ggtgcatcgg acgagtggaa gtacctcgac gattgagccg atttacttcg
     3421 cgggcggcgg cacgcccttg tcgctcgctg caccgctcgg ctcgtccctc gcgatcgagg
     3481 gccactcgtc atcgtcatcg tcgaccacct gcgcgggcgg tggcggttgg ggcggtttgc
     3541 tctcacccat cgcgaaggat tgcggtgcag ttggctcttt cgtacgcggt ggaagcgtcg
     3601 ccgcgacgat cggtggtggc gatgtccggc tggtggtgcg ctccggcatc ggtaccatcg
     3661 gcgcagactt gacctcgacg gacttggcgt cgaccggctt gacctcgacg ggcttgacgt
     3721 cgacgggctt cgcctcgacg ggcttcgcca cgatcggagc tttcagttca ggctcgcctt
     3781 gcagctgcaa cggcgaaaac accgtacgcg cgtgcgcggg caggacctcg aacttgcgca
     3841 cgaggtcggc gatcggaacg aagatctcct cgcgtcggtt accggcgatc aaccggcgca
     3901 atacgagctc gccatcactg cgcgagtgct tgttgatctg ctgtacggtg gggccaatgg
     3961 cagctgtcaa tcgctcggcg agcacgcgcg ggttggcctg atcggccatc ggcttgccgt
     4021 cgatctcgat cgagacgaga ttgccgcgag cgtcggcgag tgatcgggtc gcggcttcga
     4081 gcttttcgac gagcgaacgc aatccggcgc ggtcgcgctc ttcgatcgag acttcctttg
     4141 ccggactgcc gcccgtggat tcgatcgaca tgtcaccggt cggcgccgcc ctgatgatca
     4201 attctccgcc gttgccttcg gctcgcaact ggatgcaaac aatcgctccg acggtgacgc
     4261 tgaccacctg gtaccgaccg agcttgtgcg cgacgagctt gtcggatttc ttcagccagc
     4321 cgccggcctc gcggccatgg acctcgacgc cttctgcgac acggtcgact cgcagatccg
     4381 cggtgaacaa cgaggtcggc ggaatctcga gggcgagcac cgcttcgacg gcaaagctcg
     4441 tgcgttggcg catcgtcgct ttcatgccgg tccccgaccg cacgacctcg agctctttga
     4501 cggcacccgg aagatcatgc gtacggagca gcttctcgag cgcttctttc gcgcgcgtgt
     4561 tgatgccctg gctgttcttg gcgagctcgt cgcgttcggc agcgagcttg gccttcgtct
     4621 cggccgattc cgattcgacc gcggcgcgga tcgccctcgc gatggtggcg gcgcagcgac
     4681 cggccgggct cccggcctgc tggtccgcga cgggaccaac gaactcggcg gcacgtttgc
     4741 cgaactcggc gacggcggtt agctgcgact ctatttcgcg ctcgcgcgtc cgttttttct
     4801 cgtcgaccgc ggccagcgcg acgtcgcatt cgaccatcac gactgcgcaa tcgatgacct
     4861 cttgcagaaa cgcgaggtag tcgaagtcca gattggactc cgtcga
//
LOCUS       DASUFX010000843         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_555233,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000843 DASUFX010000000
VERSION     DASUFX010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555233"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFV99_33900"
     CDS             complement(<1..427)
                     /locus_tag="VFV99_33900"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HEX5064435.1"
                     /translation="MSEPSTPSITSEVQPALRRRLARHRRLVKLGGIIVLAGALLIPL
                     ALLVPVVEERATLRDGVVAEIERDWGKQQTIIGPVVVVPLEKGVAHVFPDELHAKTVL
                     APEQRSRGIYAAVVYTATVELAGTFHRPTPAELGVPAEQV"
     gene            complement(557..2503)
                     /gene="mnmG"
                     /locus_tag="VFV99_33905"
     CDS             complement(557..2503)
                     /gene="mnmG"
                     /locus_tag="VFV99_33905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5064436.1"
                     /translation="MRAVSFEYPDSYDVIVVGAGHAGCEASLAAARMGARVLVMTGSL
                     EMVAQMSCNPAIGGVAKGHLVKELDALGGEMASVIDQTGIQFRRLNASKGPAVRSTRA
                     QADKRRYRDEMKFRLEQQERLSLRQGEVAKLLVEDGRMVGVATTMGVTYRARAVILTT
                     GTFLRGAIFVGEARAAGGRAGEAPAVGLSHSLAELGFPLARLKTGTPCRLDRKTLDVQ
                     GLELQPGDDPTPMFRWAGVKAPPLRQIACWITYTNDKTHAIIRDGLPRSPLYRKEIAG
                     TGPRYCPSIEDKIVRFADKDRHQIYLEPEGLDTGEVYPNGISTSLPFDIQLAFIRTIP
                     GLERAEMTRPGYAVEYDFIDPREVWPTLETRRVGGLYFAGQINGTSGYEEAAIQGFLA
                     GANAAIALGFGPDKEPLILRRDQAYMGVLVDDLTTRGTKEPYRMLTSRAEFRLLLRED
                     NAVDRLMPIGRKLGLVDDERWLVFEAWRAEIEAAHTRARDASVLGTDAVNVALAKFGS
                     SPIVNRRASLAELCKRPELDWRAVEEIAAAGGISAWTGSEAALERVEIELCYDGYLRR
                     QEADAAKLAKADNVKVPEGIVYKDIPGLSNEVVEKLEAIRPRSVGQASRISGVTPAAV
                     AILMTHIGLVHRRRSASSTRDSVT"
     gene            complement(2551..3231)
                     /locus_tag="VFV99_33910"
     CDS             complement(2551..3231)
                     /locus_tag="VFV99_33910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064437.1"
                     /translation="MRFFWLCLLAGCSGGAHQIEIGAPPAKVTEGVYAGPLCSGDTCK
                     CASGPADGGAGVPEDDKKRFEVRLNSPNELWLQVGDNRMYKDAERVEACWYMDLAAPG
                     ETAVAMRASKEHGVSATWSIRELGTKTKSYYDTLVFGCGNPGACSFEELDTKKAELKD
                     PKHDHCGSTIVKGLTWDTGRSPDQQYPNELLVTAKLKIYKFVPDRPHGEDCAKKQTEE
                     HDEDNPKM"
     gene            3328..4116
                     /gene="bamD"
                     /locus_tag="VFV99_33915"
     CDS             3328..4116
                     /gene="bamD"
                     /locus_tag="VFV99_33915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5064438.1"
                     /translation="MLLRLLVVAALVSGAACGAKTGSGSVDYSVSAQKNYEKGLKELE
                     NKDWVAASKYFGFIKSRFPYSKYAVLAELRLADAEFGAEQYLESIDSYRLFIKFHPTH
                     EMVANGYASFRIGEAYFKQLPGDFWMFPPSYEKDMSSTEDAATELKSFLDKYPDSPYR
                     EKATDILKKTGKRLADHEWYVARYYWDRGKPMGTVLRLRRLLEQYRGVGYDEEALWLL
                     GRAYVAVDMPDRARQSWTELVDKYPRSERAGDAREALATLSPKR"
BASE COUNT          668 a         1361 c         1402 g          718 t
ORIGIN      
        1 ggacctgttc agcggggacg ccgagctcgg ccggcgtcgg gcgatgaaac gttcctgcaa
       61 gctcgacggt cgccgtatag acgacggccg cgtagatacc gcggctgcgt tgctcgggtg
      121 cgagcaccgt cttggcgtga agctcgtcgg ggaacacgtg cgcgacacct ttctcgagcg
      181 gaacgacgac gaccggtcca atgatcgtct gttgctttcc ccagtcgcgc tcgatctcgg
      241 cgacgacgcc gtcgcgaagc gtcgcgcgct cctcgacgac gggcacgagc aacgcgagtg
      301 gaatcaagag cgcgcccgcg agcacgatga tgccgccgag cttcacgagc ctgcgatgac
      361 gcgccagccg gcgacgaagc gctggttgga cttcggacgt gatcgagggc gttgacggct
      421 cgctcatctg cgggcaacgc acgacgctgc gaagctattc acttgggctg tcggtgccga
      481 gcgcgcgttg tcgaacagtc acatcgagga caatttgacg ttttccgtcc gcactaacct
      541 tccagcgtcg agcaggtcag gtgaccgaat cgcgagtgct cgaggctgaa cgacgcctat
      601 gcacgaggcc gatgtgcgtc atcaggatcg cgaccgcggc aggcgtgacg ccgctgatcc
      661 gcgatgcctg gccgacggag cgcgggcgaa tcgcctcgag cttctcgacg acctcgttcg
      721 acaggcccgg gatgtctttg tagacgatgc cctcgggcac cttcacgttg tcggccttcg
      781 cgagcttcgc ggcatcggct tcctggcggc gcagataacc gtcgtagcag agctcgatct
      841 cgacgcgctc gagcgcggcc tcgctaccgg tccacgcgga gatgccgccg gcggctgcga
      901 tctcttcgac cgcgcgccag tcgagctcgg gccgcttgca cagctcggcg agcgacgcgc
      961 ggcgattgac gatcggtgag ctgccgaact tcgcgagcgc gacgttgacc gcatcggtgc
     1021 cgagcacgct ggcgtcacgc gcgcgcgtgt gggcggcttc gatctcggcg cgccatgctt
     1081 cgaatacgag ccagcgctcg tcgtcgacga ggccgagctt gcggccgatc ggcatcaggc
     1141 gatcgaccgc gttgtcttcg cgcagcagca ggcggaactc cgcgcgcgag gtcagcatgc
     1201 ggtacggctc cttcgtgccg cgcgtggtga gatcgtcgac gagcacgccc atgtacgcct
     1261 ggtcgcggcg caggatcagc ggctccttgt cggggccgaa gccgagcgcg atcgccgcgt
     1321 ttgcgccggc gaggaagccc tggatcgcgg cctcctcgta gccactcgtg ccgttgatct
     1381 ggcctgcgaa gtaaaggccg cccacgcgac gcgtctcgag tgtcggccag acttcgcgcg
     1441 gatcgatgaa gtcgtactcg accgcgtaac cggggcgcgt catctcggcg cgctcgaggc
     1501 cggggatcgt gcggatgaat gcgagctgga tgtcgaacgg cagcgacgtc gagatgccgt
     1561 ttggatagac ctcaccggtg tcgaggccct cgggctcgag atagatctgg tgacgatcct
     1621 tgtcagcgaa gcggacgatc ttgtcttcga tcgacgggca gtagcgcgga ccggtgcccg
     1681 caatctcctt gcgatagagc ggcgagcgcg gcaggccgtc gcgaatgatc gcgtgcgtct
     1741 tgtcgttcgt gtacgtgatc cagcacgcga tctggcggag cggcggcgcc ttgacgccgg
     1801 cccagcggaa catcggtgtc gggtcgtcgc ctggctgcag ctcgaggcct tgcacgtcga
     1861 gcgtcttgcg atcgagacgg cacggcgtgc cggtcttgag ccgcgcgagc ggaaagccga
     1921 gctcggcgag cgagtgcgag agaccgacgg cgggcgcctc gcctgcccgg ccacctgctg
     1981 cgcgcgcttc gccgacgaag atcgcgccgc ggaggaatgt gcccgtggtg aggataacgg
     2041 cgcgcgcgcg ataggtgacg cccatcgtcg tggcgacgcc gaccatgcgg ccgtcttcga
     2101 cgaggagctt cgcgacttcg ccctggcgca gggagaggcg ttcctgctgc tcgaggcgga
     2161 acttcatctc gtcgcgatag cggcgcttgt cggcctgcgc gcgcgtcgag cgcacggccg
     2221 ggcccttgga ggcgttgagc cggcggaact ggatgccggt ctgatcgatc acgctcgcca
     2281 tctcgccacc gagcgcgtca agctccttga cgaggtggcc cttcgcaacg ccgccgatcg
     2341 ccggattgca cgacatctgc gcgaccatct cgaggctgcc cgtcatcacg agcacgcgcg
     2401 cgcccatgcg tgcagccgcg agcgatgcct cgcaaccggc atggccggca ccgacgacga
     2461 tcacgtcgta gctatcgggg tactcgaacg acacggcccg catacctacc atggtcggcg
     2521 ccggcggcag cgacgaggtt gcaaccctgc tcacatcttc gggttgtctt cgtcgtgctc
     2581 ttctgtttgc ttcttcgcgc agtcctcgcc gtgcgggcga tcgggcacga acttgtagat
     2641 cttgagcttc gcggtcacga gcagctcgtt ggggtactgc tggtccggcg agcggccggt
     2701 gtcccaggtc aggcccttca cgatcgtcga gccgcagtga tcgtgcttgg gatccttgag
     2761 ctcggccttc ttggtgtcga gctcctcgaa cgagcacgcg cccgggttgc cgcagccgaa
     2821 tacgagcgtg tcgtaatagc tcttggtctt cgtgccgagc tcgcgaatcg accacgtcgc
     2881 cgagacgcca tgctccttcg acgcccgcat cgcgacggcc gtctcgcccg gcgcggccag
     2941 gtccatgtac cagcaagcct cgacccgctc ggcgtccttg tacatccgat tgtcgccgac
     3001 ctggagccac agctcgttgg gcgagttgag gcggacctcg aagcgcttct tgtcgtcctc
     3061 cgggacgccc gcgccgccat cggccgggcc cgacgcgcac ttgcaggtgt ctccggagca
     3121 cagcgggcct gcgtacacgc cctctgtcac cttggccggg ggcgcgccga tctcgatctg
     3181 gtgggcacct cccgagcagc cggcgagcag gcaaagccag aaaaatcgca tgcctcgtta
     3241 taaggcaagc aacccaccga agtcgcacgg agtgccgagc aacccttgtc tggggggatg
     3301 ggtcagagta cagagggtca acccctgatg ctccttaggc tccttgtcgt tgcggctctg
     3361 gtttcgggtg ccgcctgcgg tgcgaagacc ggcagcgggt ccgtcgacta ttcggtctcg
     3421 gcccaaaaga actacgaaaa gggtctcaag gagctcgaga acaaggactg ggtcgccgcc
     3481 tcgaagtact tcggctttat caagagccgg ttcccgtact cgaagtacgc ggtgctcgcc
     3541 gagctccggc tcgccgatgc cgagttcggc gccgagcagt acctcgagtc gatcgacagc
     3601 taccgcctgt tcatcaagtt ccacccgacg cacgagatgg tcgcgaacgg ctacgcgtcg
     3661 ttccgcatcg gcgaggcgta cttcaagcag cttcccggcg acttctggat gttcccgccg
     3721 tcgtacgaga aggacatgtc gtcgacggag gacgcggcca ccgagctgaa gagcttcctc
     3781 gacaagtacc ccgactcgcc gtatcgcgag aaggcgaccg acatcctgaa gaagaccggc
     3841 aagcgcctcg ccgatcacga gtggtacgtc gcgcgttact actgggatcg cggcaagccg
     3901 atgggcaccg tgctgcggct gcgccgcttg ctcgagcagt accgcggcgt cggctacgac
     3961 gaagaagcgc tgtggctgct cggccgcgcg tacgtcgccg tcgacatgcc ggatcgcgcg
     4021 cgccaatcgt ggaccgagct cgtcgacaag tatccgcgca gcgagcgcgc cggtgatgcg
     4081 cgcgaggcgc tggcgaccct gtcgccgaag cgatagccgg caattagcca tgcgcgagcc
     4141 gcctgcgat
//
LOCUS       DASUFX010000844        14882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390 k141_415885,
            whole genome shotgun sequence.
ACCESSION   DASUFX010000844 DASUFX010000000
VERSION     DASUFX010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415885"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1788)
                     /locus_tag="VFV99_33920"
     CDS             complement(22..1788)
                     /locus_tag="VFV99_33920"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5064439.1"
                     /translation="MRWLALALVLAACGRPASPARVTPSGDVIASNMLRGDYAGSKAC
                     SDCHHDIYEAWAASPMRNMTRDAATATIRAPFTGTQLKVGTDTATMLTEGQTRYMRVD
                     DANGSHRFRITKVIGGRYREDFIGLEDGGDRLEHVLPATYVFATKSWRYKGYSVMVQE
                     RPRMSFRGVWSRECIPCHNTLPYVTMLYDELYGPGLPGYQGKLSDRELPRSRTWPAQT
                     RDEVGLAHELSKEIAVVGGEEPEEQGLRAMLPAAANAMRRYLRGDHLVELGVGCEACH
                     NGAREHAADPSVTPTFEPRSALVGVSPPKGQTGTRSQWINRICAKCHTVLFTQYPWTW
                     EGGERRGQHPGGSSTNSGEGRDFQLGGCSTEMACTHCHDPHTEDTPAKLAAMATPAGN
                     TLCTGCHKQMASDKAIEIHTHHAQGSAGTSCVACHMAKKNMGLDYALVRYHRIGSPTE
                     TRRVIADRPLECALCHADYSVDKIVTTMEQWWGKHYDRDALKKLYGDDLAVNALRATL
                     ERGKPHEQAAAIGVLGGTQDRSAVEVLVPMLWHEYPLVRFYAQRALQKITGDAVAIDV
                     GAAAPDVKRAAEAWLREKIAGP"
     gene            complement(1801..2439)
                     /gene="gstA"
                     /locus_tag="VFV99_33925"
     CDS             complement(1801..2439)
                     /gene="gstA"
                     /locus_tag="VFV99_33925"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF007831.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX5064440.1"
                     /translation="MKLFYSPGACSLAPHIALREADRTFELERVDLRSRRCASGADYC
                     AINPKGQVPALQLDGPDSMVLTENAAVLQYIADLVPERGLAPPNGTFARYRLQEWLSF
                     VGGEVHKQMSPLWHPDTPALTQERLRSRVSTRFLYIAEVLTDHAFLMGETFTVADCYL
                     FAVLRWADRFEIDLQLYPNLDEYYQRILPRPSVQAALGAEGLLEHKRIRRTA"
     gene            2578..3054
                     /locus_tag="VFV99_33930"
     CDS             2578..3054
                     /locus_tag="VFV99_33930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064441.1"
                     /translation="MTAWIVIVSEGLMFATLFAAHGGAYPNPGCVEVVLALAIAAALA
                     GSSLALASGVRHVRSKRAVLATRRLTVVMLLGMTALVLELVAAALFTARDPLGLIILG
                     LHAAHVAAATALTGWATVLTRYGRVHQRGRFVIELVATYCYVVAGLWIFVGPLLAT"
     gene            3162..3722
                     /locus_tag="VFV99_33935"
     CDS             3162..3722
                     /locus_tag="VFV99_33935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064442.1"
                     /translation="MQAPQSERRRLLEKLLRELVRSEEQAIEHAPREAKRIGEEAPVD
                     ALRDVGVHALAMRPRFAQVLEGHNLSAGRGGITATLSTLRHLVTDRIHDAERAYRAAL
                     LDLRHGLDVVRVMREVARLEELFALIRWCDDWLLARRTLVARVEAQLSWFADQALVAP
                     LPSDVATFEDSSDSERDDPSAHFDRP"
     gene            4128..4751
                     /locus_tag="VFV99_33940"
     CDS             4128..4751
                     /locus_tag="VFV99_33940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064443.1"
                     /translation="MSGNAAYRDATTDHDGATKPAATPAPQSANVTIVVKGTGTIPEI
                     DPQCALDPAGQFEAHYLSTMSVSDGDVYSASVAEGSGHIQTPSGCAIPKLTVGLITDV
                     VVRGELTINTTNCETFCSAQARADGEAQCGANASAAQCRAQYEAQASAQCQTTCTTKA
                     HAIVAEVSLAGSLLGNLDADGLRAAALGDLTANLTFDHVEDADGKDL"
     gene            4812..6983
                     /gene="otsB"
                     /locus_tag="VFV99_33945"
     CDS             4812..6983
                     /gene="otsB"
                     /locus_tag="VFV99_33945"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5064444.1"
                     /translation="MHAEWQHLASAASIIAAIDLDGTLLPFAPTPQEARVDDQTADLI
                     EALANVPGMTMGIISGRPRNLVEGLAQRFPRVAFAAEHGVWRCASGVWEASLAPVPQL
                     DEIERTLTGLAKRYPGALVERKTCSVCLHWRRVEGVHHDRVAAAAEVVVDEWLETQPS
                     LERLPSVEALEVRHRAAHKGSALAWLRSHGPAGAPILAIGDDITDEDMFVSLRDTDVG
                     ILVAATPRRTQAGLRLPSIAAAHRFLRWLVDVRQSRPSQAPEELIAARPPKPAATPQL
                     IVASNRLPAAPTGDRQREVGGLVSALLPALEQTQGVWLGWSGAERDPGLKLRVEPGDS
                     FVRAQFDYPLTWRQRFYAGFCNRSLWPLLHGFMRVHYADDEWACYVDANRAYARMLMD
                     LSRGSAEIWVQDFHLMLVARELRNSGHRGRLGFYLHVPFPALDIFETLPWATQLVAGL
                     LDFDRIGFQTQRWADNFVVSARGLLGSEAEIRARNRVRVIPVGIDPDRFAEAASSNTA
                     NPNESTSLGLEVMLGGRKLILGVDRLDYSKGIPERLEAFARLLENFPEWRNQVSFVQV
                     SVPTRSDVPEYGELRSRVEALVGRINGAYGEADWVPVRYLYRSYDQETLARLYRQAAV
                     GLVTPLRDGMNLVAKEFIAAQAPEDPGVLLLSKFAGAAEHLTLALLTNPYHPDGLAAD
                     IDSALRMPREERITRNSALRAIVWRDTAAAWAKRFLDELRG"
     gene            complement(6980..7267)
                     /locus_tag="VFV99_33950"
     CDS             complement(6980..7267)
                     /locus_tag="VFV99_33950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064445.1"
                     /translation="MKRIVLLVLMAACARRSPPQVTASDAERANVELAELQQGRKLLL
                     GKCAGCHKTPMPNDHTRAEWPKMLDEMADRAKLDTMQRSLIEKYLVVMSVR"
     gene            complement(7264..7989)
                     /locus_tag="VFV99_33955"
     CDS             complement(7264..7989)
                     /locus_tag="VFV99_33955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064446.1"
                     /translation="MLSPLLMTVLSSTASADRRLFTHTYEYKTVPQGHTALEFWYTEE
                     RATWDSDTPQALEQILEIEHGLTDHWDAAVYTVFTQIAADDPLVAQPYQFHELKLETR
                     YRFADRGEWPIDVLAYGELAKEFGESVYEIEAKGIFARDFENLTVALNIIAEIETGKD
                     VPETELELGWATGVSYEVNPKFSLGVETYGKFEPEEGEVLPAIGPVIAVAPSSNLWAT
                     FTAGFGFDEAPAFSARFLLGVEL"
     gene            complement(8103..9905)
                     /locus_tag="VFV99_33960"
     CDS             complement(8103..9905)
                     /locus_tag="VFV99_33960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5064447.1"
                     /translation="MKAGIEYRTKFDTVAPSIVRAMPQHGGDIIADVLARHGVTHLFT
                     LCGGHISPILTGCQARGIKVVDVRDEGNAVFAADAVARMSGTIGAAAVTAGPGVTNTI
                     TAIKNAQMAQSPLILFGGATATLLKGRGSLQDIDQMSLMKPLVKWATTVKTVPALQAT
                     IERAIEVATEGVPGPVFVEVPVDILYPESIVRMWYEKEAGLGKAKGIGAKALELYIKG
                     HLYRQFRAPTLPELELPMKLPKLRGDNAVDKAAEAVLAAKRPVIVVGSQTLVGVKDPG
                     RLARAIGTLGAPVYLAGMARGLLGRTHELQMRHNRGQALKEADCIIICGFPFDFRLGY
                     GRGMNKTATLIAANLSSHELRKNKRPDIAIEMHAGDFLVALAERASGASAGWSEWIGT
                     LRGREQARDKEISSKVAASGELVDPIHFFSRLEEAMADDAVLVVDGGDFVATASYIVR
                     PRSPLSWLDPGVFGTLGVGGGFALGAALVRPGREVWLVWGDGSSAYTLAEFDTYVRHG
                     VAPIAVIGNDASWMQIAREQVEILGTSLGCDLRRSDYHKVAEGYGGVGLVLSDPARVD
                     ATLAEAKSIAKGGRPVCINIHLRKTDFRKGSISM"
     gene            9868..10746
                     /locus_tag="VFV99_33965"
     CDS             9868..10746
                     /locus_tag="VFV99_33965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5064448.1"
                     /translation="MSNLVLYSIPAFIALIVLELLWAKRHPEAVGYEPRDTVASLSMG
                     LANVMVSAGAKLLSIPFFAWVYQHRVLELGQPTLWWSWLVLLASEDFCYYWFHRSHHE
                     VRVLWAVHVNHHSSEHYNLSTALRQALLTPLTGPIFWAPLALVGYPPWMILTAQAWSL
                     LYQFWLHTEAIGKLGPLEYIFNTPSHHRVHHGKNVRYLDRNHAGIFIVWDRLFGTFAP
                     ERERVVYGLTKDIHTFNLVKIGFHELVAIAGDVRRAPTLRAKLGYIFAPPGWSHDGST
                     LTAGQLHKQLRASSSL"
     gene            complement(10811..11461)
                     /locus_tag="VFV99_33970"
     CDS             complement(10811..11461)
                     /locus_tag="VFV99_33970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064449.1"
                     /translation="MGASVSAGFGGTPWGDAFQAAAKPCPTPGAESCTAVESWANALL
                     FRDPVGETRAQLDKAIAFHATTVVGIDLLFWHVYNVRNVDAGLAELDKVLATGAWVIV
                     GDVPLITTASELLLPKEAIPSQAELDAANAKISAWAKTDHVLLVPLSAWTEPLRAGAD
                     VEIAPGEKVPAISLMALDGLHANALGTWYLLNRLDHFIEEKLPGTPKDALQFVRPR"
     gene            11688..13043
                     /locus_tag="VFV99_33975"
     CDS             11688..13043
                     /locus_tag="VFV99_33975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064450.1"
                     /translation="MAVHDLPHAMNSTSSNCAHLVQCWYSRCCRMGPMLGGLTPRKTN
                     MSVPHSRRAMLDTLLRHLPMVRLSVGALAALLFMGCSGLIDGGDENVSAEEQAARELF
                     VQKAKPVFDTTCASCHSGSDPSNAFLAGSTPMESRATLLASNVISLEATQSSRILTKG
                     AHNGPALIASQTSDILEWLAAERDAAGVAGTVDTGLETQPFTPVMCTGGNPGDATCPI
                     TYVDISGLVEGWAGAKIKFVAQALSQDLYVTNLSLEGGAEGVYIEHPLFVSWPPQGDP
                     IPDTLDRFFNVKMNLMGGEMPQQINGGTAAFINFTPSNQLTIHFKVVDRYRPDSGMGG
                     GGMTGNAGCKSLTTFKANAKGPLQTNCGSCHANAGNANARGAMDLTGIAATDDTTLQN
                     VCNQARTRINFQDTNASGFYIAPNPAMQTNHPFKFAAQANFDTFKAAVDVWVQAEKTA
                     P"
     gene            13062..14231
                     /locus_tag="VFV99_33980"
     CDS             13062..14231
                     /locus_tag="VFV99_33980"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5064451.1"
                     /translation="MKRISALLLVAAIAAPACSSDQGDDGMGSGSGSGSGSGSNGDEW
                     DQLLGQRVVDYNAALRIAALRLTGKLPTMAEIDQIATAADDAAKKAAYEGLIQQYMSS
                     PDFARQMFYFWRDTFKMGETAALDTAPAFAARLSVNNGSYMDMFTASADGCPTFDATA
                     GTFTSAECTNNGPKAGLLTNPGIMAHYFGNLAFRRSRFVQETFVCTKFPAEISTSPTD
                     VGGAAPYTGVWPFNSIASPTNGGGRINFQDTSAVICANCHSTLNHLTPLFAYYDVNGA
                     YQTAISVPTPLDGAPMAKMTDYLPAGETTAWRLNTPAADLPALGQAIAADPDVAECGV
                     ARMWNWALGKTDIVDTLQDVPPETIQAQVDAFKSSGFKMKDMIYAVFTSDDFVKF"
     gene            14241..>14882
                     /locus_tag="VFV99_33985"
     CDS             14241..>14882
                     /locus_tag="VFV99_33985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064452.1"
                     /translation="MTRFGYTLAILASSVLAAACTDDVPTENTPPPGSTSGNEDTTFD
                     HDNDYISPWELLDRLTKEGPPRYTSHVHSCSKVRYKTLSNVLSGVGVNVASTTALSAG
                     DLYRNGANALGGPNYANRIRENIMITTSGASKEFDIFAAAAPEVIASFAANTVARCPG
                     AQLFDAANTCRPEGISCLIGAPATSRHLDYCNLTVSSASTVDVGKRLAVAAMLA"
BASE COUNT         2586 a         4984 c         4666 g         2646 t
ORIGIN      
        1 gatcgaggag gtgccgcgca ctcatgggcc ggcgatcttc tctcgcagcc atgcctcggc
       61 ggcgcgtttc acatccggtg ccgccgcgcc gacgtcgatc gcgactgcat cgccggtgat
      121 cttctgcaac gcacgctggg cgtagaaccg aacgagcggg tactcgtgcc acagcatcgg
      181 cacgagcacc tcgaccgcgc tgcgatcctg cgttccgccg agcacgccga tcgccgcggc
      241 ttgttcgtgc ggcttgccgc gttcaagcgt tgcgcgtagc gcgttgaccg cgaggtcgtc
      301 accgtagagt ttcttgagcg cgtcgcgatc gtagtgcttg ccccaccact gctccatcgt
      361 cgtcacgatc ttgtcgacgc tgtagtcggc atggcagagc gcgcactcga gcggccgatc
      421 ggcgatcaca cgccgcgtct cggtcggtga gccgatgcgg tgatagcgaa cgagcgcgta
      481 gtcgaggccc atgttcttct tcgccatgtg gcaggcgacg cacgacgtgc cggccgaacc
      541 ctgggcgtgg tgcgtgtgga tctcgatcgc cttgtcgctc gccatttgct tgtggcagcc
      601 ggtgcacagc gtattgcccg cgggcgtcgc catcgcggcg agcttcgcgg gcgtgtcctc
      661 ggtgtgcgga tcgtggcaat gcgtgcatgc catctccgtc gagcagccgc cgagctggaa
      721 gtcgcgacct tcgcccgagt tcgtcgagct gccgcccggg tgctggcccc tgcgttcgcc
      781 gccttcccac gtccacgggt actgcgtgaa cagcacggtg tggcacttcg cgcagatgcg
      841 attgatccat tgcgagcgcg tgccggtctg gcccttcggc ggcgacacgc caacgagcgc
      901 actgcgtggc tcgaatgtcg gcgtcaccga cgggtctgcg gcgtgctcgc gcgcaccgtt
      961 gtggcaggcc tcgcagccga cgccgagctc gacgagatga tcgccgcgca ggtacctgcg
     1021 catcgcgttc gcagcggccg gcaacatcgc gcggagcccc tgctcttccg gctcctcacc
     1081 gccgacgact gcgatctctt tcgacagctc gtgcgcgagt ccgacctcgt cacgcgtctg
     1141 cgctggccac gtgcgcgagc gcggcagctc gcgatcggag agctttccct gatagcccgg
     1201 cagacctggc ccgtagagct cgtcgtagag catcgtcacg tacggcagcg tgttgtgaca
     1261 gggaatgcac tcgcgcgacc acacgccgcg gaacgacatc cgcggccgct cctgcaccat
     1321 cacggagtag cccttgtagc gccacgactt cgtcgcgaac acgtaggtcg ctggcagcac
     1381 gtgctcgagc cgatcgccgc cgtcttcgag gccgatgaag tcctcgcgat agcgaccgcc
     1441 gatgaccttc gtgatccgga agcggtgcga gccattcgcg tcgtcgacgc gcatgtaccg
     1501 cgtctggccc tcggtgagca tcgtcgcggt gtcggtgccg accttgagct gggtcccggt
     1561 gaatggcgcg cgaatcgtcg cggtcgccgc gtcgcgcgtc atgttgcgca tcggcgatgc
     1621 tgcccacgct tcgtagatgt cgtggtgaca gtcagaacat gccttcgagc cggcgtaatc
     1681 gccgcgcagc atgttgctcg cgatcacgtc accgctaggc gtcacgcgtg ccggcgacgc
     1741 gggccggccg caagctgcca agacaagggc gagcgcgagc cacctcaccc tgtggatgta
     1801 tcacgcagta cgccggatgc gcttgtgctc gagcagtccc tcagcgccga gcgccgcctg
     1861 aaccgacgga cgcggcaaaa tgcgctgata gtactcgtcg aggtttggat acagctggag
     1921 gtcgatctcg aaccgatccg cccagcgcag caccgcgaac aggtagcaat cggcgacggt
     1981 gaacgtctcg cccatcaaga acgcatggtc ggtcaacacc tccgcgatgt agaggaagcg
     2041 cgtgctgacc ctgctgcgca aacgctcctg cgtaagcgcc ggcgtatcgg gatgccacag
     2101 cggactcatt tgcttgtgga cctcgccgcc gacgaacgac agccattcct gcaagcggta
     2161 acgcgcaaac gtcccgttcg gaggcgcgag tccgcgttcg ggaacgaggt cagcgatgta
     2221 ttgcagcacc gccgcgttct cggtcagcac catgctgtca ggcccgtcga gctgtaacgc
     2281 tggcacctga ccctttggat tgatcgcgca gtagtccgcg ccgcttgcgc agcgacggga
     2341 tcggagatcc acgcgttcga gctcgaacgt acgatccgcc tcgcgcaaag cgatgtgcgg
     2401 cgcgagcgag caagcacctg gagaatagaa caacttcata cggcctctgc tctgtagcgg
     2461 agcatcggct gtgccacgca catcggacgc tgctcgatcc acggacgcaa gggcacatgc
     2521 gttgcagcgc cctggcgaat gcaaattgct ctgagtcggc acgaagagcg actacgtatg
     2581 acggcctgga tcgtcatcgt cagcgaaggc ttgatgtttg ccacgctgtt cgcggcgcac
     2641 ggtggcgcgt atccgaatcc agggtgcgtc gaggtcgtgc tcgcgctcgc gatcgccgca
     2701 gctctcgcag gctcatcact cgcgctcgca tccggcgtcc gccacgtgcg aagcaagcgt
     2761 gcggtgctcg cgacacgccg gctcaccgtc gtgatgctcc tcggcatgac ggcgctcgtg
     2821 ctcgagctcg tcgcggcggc gctgttcacg gcgcgcgatc cactcggcct gatcatcctc
     2881 ggtcttcacg ccgctcacgt cgcggcggcc acggcgctca caggctgggc caccgtgctt
     2941 acccgctatg gacgcgttca tcagcggggc cgcttcgtga tcgagctcgt cgctacgtac
     3001 tgctacgtag tcgctggctt gtggatcttc gtcgggccgc tgctcgcgac ctgattctcc
     3061 gtgacatcag cgcacgtcgg gcgcagctga cacgcaacgt gagcgccacg tggcggaaat
     3121 gctccgaaac agccggcatg cgcgctgcac aacgcgcccg tatgcaagca cctcagagtg
     3181 agcgccggcg tctgctcgag aagctgctgc gcgagctcgt gcggtccgag gagcaggcga
     3241 tcgagcacgc acctcgcgaa gcaaagcgca tcggcgagga agcgccggtg gacgcgcttc
     3301 gcgacgtcgg tgtgcacgcg ctcgcgatgc ggccgcgctt cgcgcaggtg ctcgaaggac
     3361 acaacctcag cgcgggacga ggcggcatca cggcaacgct ctcgacgctt cgccatctcg
     3421 tcaccgatcg cattcatgac gccgagcgcg catatcgtgc ggcgctgctc gatttgcgcc
     3481 acggcctgga tgtcgtgcgc gtgatgcgag aggttgcgcg cctcgaagag ctcttcgcac
     3541 tcattcgttg gtgcgacgat tggttgctcg cacgccgcac acttgtcgcg cgcgtcgagg
     3601 cacagttgag ctggttcgca gaccaagcgc tcgtcgctcc gcttccttcg gacgtcgcaa
     3661 cgttcgaaga ttcaagcgac tcagagcgcg atgatccctc agcgcatttc gatcgcccgt
     3721 gaccgccgtg gcatgtcgat cgcagtaacc gacttcgggc acaccaagaa caatcacacg
     3781 caaagcgaaa gcaaacgtgt attgagtcgc agagacgagg ttcgtgcgac gcggtgattc
     3841 acgatggcga ggtcacggag aatcctgcac ttggttgtgc cttttttatt tccgtaaggt
     3901 cgcctcaccg tcaggcgacc ttactgacgt cctcgccagt ccgacatgca gaaatggtcg
     3961 cgatggtgat cacgttcagc gtgcaaccgt cgtgaatcac gcatgcgaag gatcggcatg
     4021 ctcgccgcaa tatccagcga gctatgtcga gaacaaatct tattatcatc ctcgcttcgg
     4081 ctctcgggtt tggttgcacg aatgacgaga gtcaaagcag ctcgagcttg agcggcaatg
     4141 cggcctatcg cgatgcgacc accgatcacg acggcgcgac gaaaccggcg gcaacgccag
     4201 cgccgcaaag cgcgaacgtc acgatcgtcg tcaaaggcac cggcacgatc ccggagatcg
     4261 atccgcaatg cgcgctcgat cccgcgggtc agttcgaagc gcactacttg tcgacgatga
     4321 gtgtcagcga tggcgacgtc tacagcgcga gcgtcgccga aggcagcggt cacatccaga
     4381 cgccatcggg ctgcgcgatt ccgaagctga cggtcggcct catcaccgac gtcgtcgttc
     4441 gcggcgagct caccatcaac acgacgaact gtgaaacgtt ctgttctgca caagcgcgtg
     4501 ccgatggcga ggcgcagtgc ggagcgaacg caagcgccgc gcagtgccgc gcgcaatacg
     4561 aggcgcaagc gagcgcgcag tgtcagacga cctgcacgac gaaagcgcac gcgatcgtcg
     4621 cggaggtgtc gctcgcgggc tcgctgctcg gcaatctcga tgcagacggc ctacgcgcgg
     4681 cagcgctcgg tgacctcacc gcgaacctca cgttcgacca cgtcgaggac gcggacggta
     4741 aggacctgta gccgagcgtt aggcccggcg aggcggtttg attcactccg tgaccgcggt
     4801 aaggtgcgga catgcacgcc gagtggcagc acctcgcctc ggcagcatcg atcatcgcag
     4861 cgatcgatct ggatggcacg ctcttgccat tcgcccccac gccacaggag gcaagggtcg
     4921 atgatcaaac cgctgatctg atcgaggcgc tggcgaacgt gcctggcatg acgatgggca
     4981 tcatctcggg ccggccgcgc aatctggtcg agggccttgc gcagcggttc ccgcgcgtcg
     5041 cgttcgcggc cgagcacggt gtatggcgat gcgcatccgg cgtgtgggaa gcatcgctcg
     5101 cgccggtgcc gcagctcgac gagatcgagc gcacgttgac cggcctcgcc aagcgctatc
     5161 ccggcgcact tgtcgagcgc aagacgtgct cggtgtgctt gcactggcgg cgtgtcgaag
     5221 gcgtgcatca cgaccgcgtt gccgctgcgg ccgaagtcgt cgttgacgaa tggctcgaga
     5281 cgcagccgtc gctcgagcga ttgccgagcg tcgaagcgct cgaggtgcgt caccgcgccg
     5341 cccacaaggg ctcggcgctc gcgtggctgc gcagccatgg cccggccggt gcgccgatcc
     5401 tcgcgatcgg cgacgacatc accgacgaag acatgtttgt cagcctgcgc gacaccgacg
     5461 tcgggatcct cgtcgctgcc acgccgcgcc gtacgcaagc cgggctgcgg ctgccgtcga
     5521 tcgcggccgc gcatcgcttc ctgcgctggc tcgtcgacgt acgccaatcg cgcccgagcc
     5581 aggcgcccga agagttgatc gccgcgcggc ctccgaaacc ggcagcgacg ccgcagttga
     5641 tcgtcgcgtc gaatcgtttg cccgccgcac cgacgggcga tcgccagcgc gaggtcggcg
     5701 gccttgtctc cgcgctgctg ccggccctcg agcagacgca aggcgtgtgg ctcggctgga
     5761 gcggtgcgga gcgcgacccc gggctcaagc tgcgcgtcga gccgggcgac tcgttcgtgc
     5821 gcgcccaatt cgactacccg ctcacgtggc gacagcgctt ctacgctggt ttctgcaacc
     5881 gtagcttgtg gccgctgctc cacggcttca tgcgcgtgca ctacgccgac gacgaatggg
     5941 cctgttacgt cgacgcgaac cgcgcatacg cgcgcatgct catggatctg tcgaggggca
     6001 gcgccgagat ctgggtgcag gactttcacc tgatgctcgt cgcgcgcgag ctgcgcaact
     6061 cggggcatcg cggtcggctc ggcttctatc ttcacgtgcc gtttccggcg ctcgacattt
     6121 tcgaaacgct gccgtgggcg acgcagctgg tcgccggcct gctcgacttc gatcgcatcg
     6181 ggtttcagac gcagcggtgg gcagacaact tcgtcgtgtc ggcacgcggc ctgctcggta
     6241 gcgaagccga gatccgtgca cgtaaccgcg tgcgcgtgat tcccgtcggc atcgatcctg
     6301 accggttcgc agaagcggcc agcagcaaca ccgcgaatcc gaacgagtcg acgtcgctcg
     6361 gtctcgaagt gatgctcggc gggcgcaagc tgatcctcgg cgtcgatcgc ctcgattact
     6421 cgaagggcat ccccgagcgg ctcgaagcgt ttgcgcgcct gctcgaaaac tttcccgagt
     6481 ggcgcaacca ggtctcgttc gtccaggtct cggtaccgac gcgttccgac gtgcccgagt
     6541 acggcgagct gcgctcgcgc gtcgaggcgc tggtcggtcg catcaacggc gcctacggcg
     6601 aagcggactg ggtgccggtg cgctacctct atcgttcgta cgatcaggag acgctcgccc
     6661 gcttgtatcg ccaagccgcc gtcggtctcg tcacgccgct gcgtgacggc atgaacctgg
     6721 tcgccaagga atttatcgcc gcgcaggccc ccgaagatcc gggcgtgctc ttgctatcga
     6781 agttcgccgg cgccgccgag cacctcacgc tcgcgctact cacgaacccc taccaccccg
     6841 acggcctcgc ggccgacatc gatagcgcac tgcgcatgcc gcgcgaggag cgcatcacga
     6901 gaaacagcgc gctacgcgcg atcgtgtggc gcgacacggc agcggcgtgg gcaaagcgct
     6961 ttctcgacga gctgcgcggt tagcgcacgc tcatcacgac gagatacttc tcgatcagcg
     7021 agcgctgcat ggtatcgagc ttcgcgcggt ccgccatctc gtcgagcatc ttcggccact
     7081 cggctcgcgt gtgatcgttt ggcatcggcg tcttgtgaca gccggcgcac ttgccgagca
     7141 agagcttgcg gccctgctgc agctccgcga gctcgacgtt cgcgcgctcc gcgtcactgg
     7201 cagtgacctg cggcggcgaa cgccgcgcgc atgctgccat caggacgagg agcacgatgc
     7261 gtttcacagc tcgactccca ggaggaagcg ggcgctgaac gcaggcgctt cgtcaaagcc
     7321 aaagcccgcg gtaaacgtcg cccacaggtt gctcgacggc gcgaccgcga tcaccggacc
     7381 aatcgcgggt agcacctcgc cctcctcggg ctcgaacttg ccgtacgtct cgacgccgag
     7441 cgagaacttg gggttgacct cgtagctgac gccagtcgcc cagccgagct cgagctcggt
     7501 ctcgggcaca tccttgccgg tctcgatctc ggcaatgatg ttgagcgcga cggtgaggtt
     7561 ctcgaaatcg cgcgcgaaga tgcccttcgc ttcgatctcg tagacgctct cgccgaactc
     7621 cttggcgagc tcgccgtacg cgagcacgtc gatcggccac tcgccgcgat cggcgaagcg
     7681 ataacgggtc tcgagcttga gctcgtgaaa ctgatacggc tgcgcgacca gcggatcgtc
     7741 ggcggcgatc tgcgtgaaca cggtgtagac ggctgcgtcc cagtgatcgg tgaggccgtg
     7801 ctcgatctcg aggatctgtt cgagcgcctg cggcgtatcg gagtcccacg tcgcgcgctc
     7861 ttccgtgtac cagaactcga gcgccgtatg cccctgcggc acggtcttgt actcgtacgt
     7921 gtgagtgaac aagcggcggt cagcggaggc ggtggacgac agcacggtca tgaggagggg
     7981 tgacaacaac aaggctactg caaaacggct cataatcgac tttgaaaata gatttcaatt
     8041 tcgttcagtt caagggcttg gcttagattt ccgctcggcc aggaaatcac cagggcttct
     8101 cgctacatcg agatcgagcc cttgcggaag tcggtcttcc gcaagtggat gttgatgcag
     8161 accggccggc cgcccttcgc gatcgacttc gcctcggcga gggttgcatc gacgcgcgcc
     8221 ggatcgctga gcaccaggcc aacgccgccg tagccttcgg cgaccttgtg atagtcgctg
     8281 cgccgcaagt cgcagccgag cgaggtaccg aggatctcga cttgctcgcg cgcgatctgc
     8341 atccacgacg cgtcattgcc gatcaccgca atcggcgcga cgccgtggcg aacgtacgtg
     8401 tcgaactcgg cgagcgtgta cgcgctcgag ccatcgcccc acacgagcca tacttccctg
     8461 cccggccgca cgagcgcagc gccgagcgcg aaaccgccgc cgacgccgag cgtgccgaac
     8521 acgccgggat ccagccacga caacggcgag cgcggccgca cgatgtagct cgcggtcgcg
     8581 acgaagtcgc cgccatcgac gacgagcact gcgtcgtcgg ccatcgcttc ctcgaggcgc
     8641 gagaagaagt ggatcggatc gacgagctcg cccgatgctg cgaccttcga cgagatttcc
     8701 ttgtcgcgtg cctgctcgcg gccgcgcaac gtcccgatcc attcgctcca ccccgcgctc
     8761 gcacccgacg cgcgctcggc gagcgcgacg aggaagtcgc ccgcgtgcat ctcgatcgcg
     8821 atgtcgggtc gcttgttttt gcgcagctcg tggctggaga gattcgcggc gatcagcgtc
     8881 gccgtcttgt tcatgccgcg gccgtagccg aggcggaagt cgaacggaaa accgcagatg
     8941 atgatgcagt cggcttcctt cagcgcctga ccgcggttgt ggcgcatctg gagctcgtgc
     9001 gtgcgcccga gcagaccacg cgccatcccg gcgagataca ccggcgcacc gagcgtgccg
     9061 atcgcgcgcg cgagccgacc tggatccttc acaccgacga gcgtttggct gccgacgacg
     9121 atcaccggcc gcttcgccgc cagcaccgcc tccgctgcct tgtccaccgc gttgtcgccg
     9181 cgcagcttcg gcagcttcat cggcagctcg agctccggca gcgtcggcgc gcggaattgc
     9241 cggtagagat gtcccttgat gtagagctcg agcgccttcg cgccgatgcc cttcgctttg
     9301 ccgagacccg cttccttctc gtaccacatg cgcacgatcg actcgggata gagaatgtcg
     9361 accggcacct cgacgaacac cgggccaggc acaccttcgg tcgcgacttc gatcgcgcgc
     9421 tcgatcgtcg cttgcagcgc cggcacggtc ttcactgtcg tcgcccactt cacgagtggc
     9481 ttcatcagcg acatctgatc gatgtcctgc agcgagccgc gtcccttgag gagcgtcgcc
     9541 gtcgcgccgc caaacaggat cagcggcgac tgcgccatct gcgcgttctt gatcgcggtg
     9601 atcgtgttcg tgacgcccgg acccgcggtg acagcggcgg cgccaatcgt gccgctcatg
     9661 cgcgcgacgg catctgccgc aaagacagca ttgccctcat cgcgaacgtc gacgactttg
     9721 atcccgcgtg cttggcagcc ggtcaggatc ggcgagatat gaccgccgca cagcgtgaac
     9781 aggtgcgtga cgccatgacg cgcgaggacg tctgcaatga tgtctccgcc atgttggggc
     9841 atggcgcgta ctatagacgg ggcaaccgtg tcgaacttgg ttctgtactc gattccggcc
     9901 ttcatcgcgc tgatcgtgct cgagctcttg tgggcaaagc ggcaccccga ggctgtcggt
     9961 tacgagccac gcgacaccgt cgcgagcctg tcgatgggcc tcgcaaacgt gatggtgtcg
    10021 gcgggcgcga agctgctctc gatcccgttc ttcgcctggg tctatcagca ccgcgtcctc
    10081 gagctcggtc agcccacgct ttggtggtcg tggctcgtgc tcttggcgag cgaagacttc
    10141 tgctactact ggttccatcg cagccatcac gaggtgcgcg tgttgtgggc ggtgcacgtc
    10201 aaccaccact cgagcgagca ctacaatctg tcgacggcgc tacgccaagc gctgctgacg
    10261 ccactcaccg gtccgatctt ctgggcgccg ctcgcactcg tcggctatcc gccgtggatg
    10321 atcctgacgg cgcaagcgtg gtcactgctc tatcagttct ggctgcacac cgaagcgatc
    10381 ggaaagctcg gtccgctgga gtacatcttc aacacgccgt ctcatcaccg cgtgcatcac
    10441 ggcaagaacg tgcgttacct cgatcgtaat cacgcgggga tcttcatcgt ctgggatcgc
    10501 ttgttcggca cgttcgcgcc cgagcgcgag cgcgtcgtct acggcctgac gaaggacatc
    10561 cacacgttca atctcgtcaa gatcggcttt cacgagctcg tcgcgatcgc cggcgatgtg
    10621 cgtcgagcac cgacgctgcg cgcgaagctc ggctacatct tcgcgccgcc cggatggagc
    10681 cacgacggat cgacgctcac cgccggccag ctccacaaac agctccgtgc gtcgtcctct
    10741 ctatagagag acagcgccac ctctctatcg atagagaggc accgctctat cgataaggtt
    10801 tgtgccccaa ctatcgcggc cgcacgaatt gcagcgcgtc cttcggcgtt cccgggagct
    10861 tctcttcgat gaagtgatcg agccggttca gcaagtacca ggtgccgagt gcattcgcat
    10921 gcaggccatc gagcgccatc agcgagatcg ctggcacctt ctcgcccggt gcgatctcca
    10981 catctgcgcc tgcgcgcagc ggctccgtcc acgcggagag cggaacgagc agcacgtgat
    11041 ctgttttcgc ccacgcgctg atcttcgcgt tcgcggcatc gagctccgcc tgcgacggga
    11101 tcgcttcctt cgggagcagc agctccgatg cggtcgtgat cagcggaacg tcgccgacga
    11161 tcacccacgc gccagtcgcg agcaccttgt cgagctcggc aagccccgcg tccacgttgc
    11221 ggacgttgta gacgtgccag aacaacaggt cgatcccgac caccgtcgtc gcgtggaatg
    11281 cgatggcctt gtcgagctgc gctcgggtct cgccgacggg gtcgcggaac agcagcgcgt
    11341 tcgcccagga ctcgaccgct gtacacgatt cagccccagg cgtgggacac ggtttggccg
    11401 ccgcctggaa cgcgtcgccc catggtgtcc cgccgaatcc ggcggagacg ctggccccca
    11461 cgatcgcgat gcgcgaaatc ggtaatgccg gccctggttc attcttcggt ttgatcgccc
    11521 cacggcaccc aaacagaggg ttcagcagac ctagccaaca aattagccag accaaccgcg
    11581 gaccggagca atttaagtaa ttgatgttgc acttcatgga catgtgattt caataacttg
    11641 gaagtgggaa cagttgacct cacgtggggg tgtccacctc atgcacaatg gcggttcacg
    11701 atctcccaca tgcaatgaat tcaacaagtt cgaattgtgc acacctcgta cagtgctggt
    11761 atagccgttg ctgtaggatg ggacccatgc tgggtggtct cactccgaga aagaccaata
    11821 tgagcgttcc gcactcgcgg cgcgccatgc tggataccct gctccgccac cttcctatgg
    11881 tgcggctgtc ggtcggcgcg ctcgccgcgc tcctctttat gggctgcagt ggcctcattg
    11941 atggtggaga cgagaatgtt tccgccgaag agcaggccgc tcgcgagcta ttcgtgcaga
    12001 aagcaaagcc tgttttcgac acgacctgcg cgtcgtgcca ctcgggctcg gacccgtcga
    12061 acgcgttcct tgcggggtct acccccatgg agtcgcgcgc gacgctgctc gcttcgaacg
    12121 tgatcagcct cgaagctacc cagtcttctc gcatccttac caagggcgcg cacaacggcc
    12181 cggcgctcat cgcgtcgcaa acctcggaca tcctcgagtg gctcgcggcc gagcgtgatg
    12241 cagccggtgt cgctgggact gtagacaccg gtctggagac tcaacccttc acccccgtca
    12301 tgtgcacggg cggcaacccg ggtgacgcca cctgccccat cacctacgtg gacatctcgg
    12361 gtctggtcga gggctgggcc ggcgcgaaga tcaagttcgt cgcgcaagcg ctctcgcaag
    12421 atctctacgt caccaacctc tcgctcgagg gtggcgcgga aggcgtctac atcgagcacc
    12481 cgctgttcgt gtcgtggccg ccgcagggcg acccgatccc ggacacgctc gaccgcttct
    12541 tcaacgtgaa gatgaacctg atgggcggcg agatgccgca gcagatcaac ggcggcacgg
    12601 cggccttcat caacttcacg ccgtcgaacc agctcacgat tcacttcaag gttgtcgatc
    12661 gctatcgccc ggactcgggc atgggcggcg gcggcatgac cggcaacgcc ggctgcaagt
    12721 cgctcacgac cttcaaggca aacgcgaagg gcccgctgca aacgaactgc ggtagctgcc
    12781 atgcgaacgc gggcaacgcg aatgcgcgcg gcgcgatgga cctcaccggc atcgcggcga
    12841 ccgacgacac gacactgcag aacgtctgca atcaggcgcg cacgcgcatc aatttccagg
    12901 acacgaatgc gagcggcttc tacatcgcgc ccaacccggc gatgcagacg aaccatccgt
    12961 tcaaattcgc cgctcaagca aacttcgaca ccttcaaagc ggccgtggat gtctgggtcc
    13021 aggccgagaa aactgccccg tagtttccga aaaggagccc catgaagagg atctcagcac
    13081 tactcctggt tgccgcaatc gcggctccgg cctgcagctc cgaccaagga gatgacggca
    13141 tgggttccgg atctggatca ggatccggtt ccggttccaa cggcgacgaa tgggaccagt
    13201 tgctcggtca gcgcgtcgtt gactacaacg ccgccctccg catcgctgct ctccgcctga
    13261 ccggcaaact gccgacgatg gccgagatcg accagatcgc gaccgcggcc gacgacgctg
    13321 ccaagaaggc ggcgtacgaa gggctcatcc agcaatacat gagctcgccg gacttcgcgc
    13381 ggcagatgtt ctacttctgg cgcgacacct tcaagatggg tgagaccgcg gcgctcgaca
    13441 ccgcgccggc gttcgctgct cgtctgtcgg tgaacaacgg cagctacatg gacatgttca
    13501 cggcttcggc cgacggctgc ccgacgttcg atgcgacggc tggcacgttc acgtcggcgg
    13561 agtgcacgaa caacggtccg aaggccggcc tcctgacgaa cccaggaatc atggcccact
    13621 acttcggcaa cctggcgttc cgtcgctcgc gcttcgtgca agagacgttc gtctgcacga
    13681 agttcccggc ggaaatctcg acgtcgccga ccgatgtcgg cggcgcggcg ccctacaccg
    13741 gcgtgtggcc gttcaactcg atcgcgagcc cgaccaacgg cggcggtcgc atcaacttcc
    13801 aggacacgtc ggcagtcatc tgcgcgaact gccactcgac gctcaaccac ctcacgccgc
    13861 tcttcgcgta ctacgacgtg aacggcgcgt atcagacggc catctcggtg ccgacgccgc
    13921 tcgacggcgc gccgatggcg aagatgaccg actacctgcc ggcgggtgag accaccgcgt
    13981 ggcgcttgaa cactccggcg gcggatctgc cggccctcgg tcaagcgatc gcggccgacc
    14041 ccgacgtggc tgagtgcggc gtcgcccgca tgtggaactg ggcgctcggc aagacggaca
    14101 tcgtcgacac cctgcaggac gttccgcccg aaaccatcca ggcccaggtc gatgccttca
    14161 agagctccgg cttcaagatg aaggacatga tctacgccgt cttcaccagc gatgacttcg
    14221 tcaagttcta ggaggagcca atgacccgct ttggatacac ccttgcgatc ctggcctcgt
    14281 cggtcttggc tgctgcctgc accgacgatg tgcccaccga aaacacccca ccgcccggct
    14341 cgacgtcggg taacgaagat acgaccttcg atcacgacaa cgactacatc tccccgtggg
    14401 agctgctcga tcgtctgacg aaggaaggtc cgccgcgcta cacgtcgcac gtgcacagct
    14461 gctcgaaggt ccgttacaag accctcagca acgtgctctc gggcgtcggc gtcaacgtgg
    14521 cgagcacgac cgcgctgtcg gccggcgatc tgtaccgcaa cggcgccaac gcgcttggcg
    14581 gtccgaacta cgcgaaccgc atccgcgaga acatcatgat caccacgtcg ggtgcgtcga
    14641 aggagttcga catcttcgcc gccgccgcgc ctgaagtgat cgcgtcgttc gccgcgaaca
    14701 cggttgctcg ctgcccgggt gctcagctgt tcgatgcagc gaacacctgc cgcccggaag
    14761 gcatcagctg cctcatcggt gcgccggcga cgagccgtca ccttgactac tgcaacctaa
    14821 ccgtgtcgag cgcatcgacc gttgacgtcg gcaagcgcct tgccgtcgcg gccatgctgg
    14881 ct
//
LOCUS       DASUFX010000845         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1943429, whole genome shotgun sequence.
ACCESSION   DASUFX010000845 DASUFX010000000
VERSION     DASUFX010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943429"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFV99_33990"
     CDS             <1..956
                     /locus_tag="VFV99_33990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064453.1"
                     /translation="WHVQHYVTPTQTLEQLKEERMLSVPELETTAHIDGTKTVQTHVK
                     TGPLDPSATARLLWCLGSMGALVMSPEVHDLATAPRRALDESRNHLRARTQWAARTSY
                     FDVLEISPLAEYPEIEAGYRLVGARYSPKALSRFDLAELAGLGKPLWEQVEKARMTLV
                     DHAMRGRYTEWLRQHLAEIDTVWAIEEHRIKDAAEAFARGQQTLASGDVHRAMGEFAR
                     ACRNHPGHPDYEANLAWVRLRVQVAAGKDQREVAAEERQNVEAYLAGRRPWPRALVAL
                     ALLCAAAGDADAARWHLHIALQIDPNVPAAAQLAQRLGMRR"
     gene            991..2469
                     /locus_tag="VFV99_33995"
     CDS             991..2469
                     /locus_tag="VFV99_33995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX5064454.1"
                     /translation="MRDIEDRLHAAPAGAIEAELVAIALEQTGAEHGALFLWDKDANG
                     LVLVHHVVGGVIVTLPRVVRHDGPRAGIAMHVYDTDQAMLVPDTSREDLYTRYLIDVG
                     CIVAAPINYQRRPLGVLTVSTREPRGLADKDLRAITAIAEVAAPLLRRVQVDKLSRAA
                     TGRPLMIKGLSTAWHEVERKLELAAPTNAPILIRGESGTGKDLVARAIHWNSRRAERP
                     YVTVNCAAIPETLLESILFGHVKGAFTGASFHKVGEFQKANGGTLFLDEVGELPVMLQ
                     AKVLRAVEQGEVQPLGSNEPPDRVDVRLICATNRDLEAMAKTGAFRDDLFYRLSVMTL
                     DLPPLRSYKDNLEVLANVFLEQSSTAHAKPAPRLGPDVLARMQAYDFPGNVRELKNTI
                     EHAVILCTGDELRVEDLPRPLQDPRPRAERARTQPQQTLEEAREKWLDPLERQYLTEL
                     LAKHGGNVKEAARAAGINTVTLYRLLKRRGLHLGRTVGTKSN"
     gene            complement(2466..3350)
                     /locus_tag="VFV99_34000"
     CDS             complement(2466..3350)
                     /locus_tag="VFV99_34000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064455.1"
                     /translation="MRHVLLIAMLAGCHGGAKTEGECKTEATAVGDLLVAAAKEPPSP
                     LTITDDVHLVTRTDLPTNRDLRQAPVVTVTPTTLKLDDKDVADTDGLAIGLRGRFEKV
                     QADLDSGRMRPAWIGDPRRVYLAIDPTTPWERVANVVASAGAAGLTAPAFVFEQPTSL
                     KPPPRAEIDDKLDALMKDDPANRASEVAQLMSKVIDHCPALKKQFGAVASEEGDKGMT
                     LSLAVAPALIECKCQVDIPNLRAVMFRILYVKRPLRFVTFEADAAKEKIEMPKPTTWA
                     EASKRFTPTLKNAELVAR"
     gene            complement(3347..4084)
                     /locus_tag="VFV99_34005"
     CDS             complement(3347..4084)
                     /locus_tag="VFV99_34005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064456.1"
                     /translation="MKRIAVLVVLLLACGKRNDLPEEAWGVADFEREGLHVDKPWTTD
                     DFTQAQAVLERVSKDHRERLPRYRGAKSGAVFAKLVTELPEDATQSIGDKFIAHANRV
                     DALNQISKLYMLNAMAVPPREWIELMGAILREAVVLTQTSEPFLASFGPDDPKRENRL
                     AGLARMKEGYGQMLLGSFLVADDKRVPEADKIALVQYATAALPVLLPASTPDAQSKIR
                     EVLAKEQAGVPAGPLHDAIVAANKALP"
     gene            4105..4518
                     /locus_tag="VFV99_34010"
     CDS             4105..4518
                     /locus_tag="VFV99_34010"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX5064457.1"
                     /translation="MSWTCPDCKRSFGRKNQSHGCAPSGTVDDYFATRPAVLRKTFDA
                     VARHLMKRDDVHIDAVTVCVMFKRERSFAEVRAKRDRLDLWFLLSRTVDDPRIHRALK
                     LSAHRTAHVVQLMRPADVDRDVREWLSEAYASSPT"
     gene            complement(4600..>5159)
                     /locus_tag="VFV99_34015"
     CDS             complement(4600..>5159)
                     /locus_tag="VFV99_34015"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEX5064458.1"
                     /translation="TSRTGALNGILSVLDNGVDPAQYQVNKTASGKYNLRLRAANYEI
                     IAFTQAYSTKSSATRAITSCVNAVTSYLDKVEANTSGARVEVLESTAGFRFNLHAQNG
                     QTVLSSESYTTKAAAWNGAFAIQDAAALDGSFAIKTATDNRFYFTLTAENGQVVGVSQ
                     MYTTKAAAQNGIASVKSLLDGMDLL"
BASE COUNT          846 a         1719 c         1720 g          874 t
ORIGIN      
        1 gctggcacgt gcagcactac gtgacgccga cgcagacgct cgagcagctc aaagaagagc
       61 gcatgttgag cgtgcccgag ctcgagacca ccgcgcacat cgacggcacg aagaccgtgc
      121 agacgcacgt gaagaccggc ccgctcgatc catcggcgac cgcgcgcctc ttgtggtgcc
      181 tcgggtcgat gggcgcactc gtgatgtcgc cggaggtgca cgatcttgca accgcgccgc
      241 ggcgcgctct cgacgagagt cgcaaccatc tgcgggcgcg cacccagtgg gccgcgcgga
      301 cgtcgtactt cgacgtgctc gaaatctcgc cgctcgccga gtatccggag atcgaggccg
      361 gatatcgact cgtcggtgcg cgctactcgc cgaaggcgct gtcgcggttc gatctcgccg
      421 agctcgcagg actcggcaag ccactctggg agcaggtcga gaaggcgcgc atgacgctcg
      481 tcgatcacgc gatgcgcggt cgctacaccg agtggctgcg ccagcacctg gccgagatcg
      541 acaccgtgtg ggcgatcgag gaacaccgga tcaaggacgc ggcggaagcg ttcgcgcgcg
      601 gccagcaaac gctcgcgtcc ggcgacgtcc atcgcgcgat gggcgaattc gcgcgcgcgt
      661 gtcgcaatca tcccgggcat cccgactacg aggcgaacct cgcgtgggtg cgcctgcgcg
      721 tgcaggtcgc ggccggcaaa gaccagcgcg aggtcgcggc tgaggagcga cagaacgtcg
      781 aggcgtatct cgccggacgt cggccatggc cccgcgcgct cgtcgcgctc gcgctgctct
      841 gtgcggcggc tggcgatgcc gatgctgcac gctggcacct gcacatcgcg ctgcaaatcg
      901 atccgaacgt gccggcggcg gcgcagctgg cgcagcggct cgggatgcgc cgctagccac
      961 ggctccgcgc agtaacgcta tagtcatggc gtgcgcgata tcgaggaccg gctacacgcc
     1021 gcaccggcag gtgcgatcga agccgagctc gtcgcgatcg cgctcgagca gaccggcgcc
     1081 gagcacggcg cgctgttcct gtgggacaag gacgcgaacg gtctcgtgct cgtgcatcac
     1141 gtcgtcggcg gcgtgatcgt cacactgccc cgggtcgtcc gtcacgatgg tccgcgcgcc
     1201 ggcatcgcga tgcacgtcta cgacaccgac caggcgatgc tcgtccccga cacgtcgcgc
     1261 gaggacttgt acacgcgcta cctgatcgac gtcggctgca tcgtcgccgc accgatcaac
     1321 taccagcgcc ggccgctcgg cgtgctcacg gtatcgacac gcgagccgcg cgggcttgcc
     1381 gacaaggacc tgcgcgcgat caccgcgatc gccgaggtcg ccgcgccgct cttgcgccgc
     1441 gtgcaagtcg acaagctatc gcgtgccgca accggccgcc cgctgatgat caaaggtctg
     1501 tcgaccgcgt ggcatgaagt cgagcgcaag ctcgagctcg ccgcgccgac gaacgcgccg
     1561 attctgatcc gcggcgagag cggcaccggc aaggatctcg tcgcgcgcgc tatacactgg
     1621 aacagccgcc gcgccgagcg gccgtacgtg accgtcaact gcgccgcgat tcccgagacc
     1681 ctgctcgaat cgatcctgtt cggtcacgtg aagggcgcgt tcaccggcgc gagctttcac
     1741 aaggtcggcg agttccagaa agcgaacggc ggcacgctgt ttctcgacga ggtcggcgaa
     1801 ttgcccgtga tgctgcaggc gaaggtgctg cgcgcggtcg aacaaggcga agtgcagccg
     1861 ctcggttcga acgagccccc cgatcgcgtc gacgtgcgat tgatctgtgc aacgaaccgc
     1921 gatctcgaag ccatggcgaa gaccggcgca tttcgcgacg acttgtttta ccgcctcagc
     1981 gtgatgacgc tcgatctgcc gccgctacgc agctacaagg acaacctcga ggtgctcgcg
     2041 aacgtatttc tcgagcaatc gtcgacggcg cacgcgaagc cagcaccgcg cctcggtccc
     2101 gacgtgctcg cgcgcatgca ggcgtacgac tttcccggca acgtgcgcga gctcaagaac
     2161 acgatcgagc acgcggtgat cctgtgcacc ggcgacgagc tgcgcgtgga ggatctgccc
     2221 cgcccgctcc aggatccgag gccccgcgcc gagcgcgcgc ggacgcagcc gcaacagacg
     2281 ctcgaagagg cgcgcgagaa atggctcgac ccgctggagc gtcagtacct gacggagctg
     2341 ctcgcaaagc acggcggcaa cgtgaaggag gccgcgcgcg ccgccggcat caacacggtc
     2401 acgctctatc gcttgctcaa gcgacgtggt ctgcacctcg gccgaacggt cggcacgaag
     2461 tcgaactagc gggcgacgag ctctgcgttc ttcagcgtgg gcgtgaagcg tttactcgct
     2521 tccgcccacg tcgtcggttt cggcatctcg atcttctcct tcgccgcgtc ggcctcgaac
     2581 gtcacgaagc gcaacggccg cttcacgtaa agaatgcgga acatcaccgc gcgtaggttc
     2641 gggatgtcga cctggcactt gcactcgatc agcgccgggg cgacggcgag agacaacgtc
     2701 atccccttgt cgccttcttc ggaggcgacc gcgccgaact gtttcttcag cgccgggcag
     2761 tggtcgatga ccttgctcat gagctgcgcc acctcgctcg cgcggttcgc cgggtcgtct
     2821 ttcatcagcg catcgagctt gtcgtcaatc tcggcgcgcg gcggcggctt gagcgacgtc
     2881 ggctgctcga acacgaaggc gggcgcggtc agtcccgcgg caccggcgct cgcgaccaca
     2941 ttcgcgacgc gttcccaggg tgtcgtggga tcgatcgcga ggtacacgcg ccgtgggtcg
     3001 ccgatccacg cggggcgcat gcggccagag tccaggtcgg cctggacctt ctcgaagcgc
     3061 ccgcgtagcc cgatcgcgag cccgtcggta tccgcgacgt ccttgtcgtc gagcttcagc
     3121 gtcgtgggcg tgaccgtgac gaccggcgcc tgccgcagat cgcggttggt cggcagatct
     3181 gtccgggtga cgagatgaac atcatcagtg atcgtgagcg ggctcggcgg ctctttcgcg
     3241 gctgcaacga gcagatcgcc gacggcggtc gcctcggtct tgcactcgcc ttcggtcttc
     3301 gcaccgccgt ggcaaccagc gagcatggca atcaggagta cgtgtctcat ggcagtgcct
     3361 tgttggcagc gacgatcgca tcgtgcagcg ggccggccgg aacccccgcc tgctctttcg
     3421 cgagcacctc gcggatcttg ctctgcgcgt cgggcgtcga tgccggcagc aagaccggca
     3481 gcgcggcggt cgcgtactgg acgagcgcga tcttgtccgc ttcaggcacg cgcttgtcgt
     3541 cggcgacgag gaacgagccg agcaacatct ggccgtagcc ctctttcatc ctcgcgagac
     3601 cggcgagccg attctcgcgc ttcggatcat cgggcccgaa cgacgcgagg aacggctctg
     3661 aggtttgcgt gagcacgact gcttctctca ggatggctcc catcagctcg atccattcac
     3721 ggggtggcac tgccatcgca ttgagcatgt agagcttcga gatctggttg agcgcgtcga
     3781 cgcgattcgc atgcgcgatg aacttgtcgc cgatcgactg cgtggcatcc tcgggcagct
     3841 cggtgacgag ctttgcgaac accgctccgc tcttcgcgcc gcgatagcgc ggcaggcgct
     3901 cgcgatggtc cttgctcacg cgctcgagca ccgcctgcgc ttgcgtgaag tcgtccgtcg
     3961 tccacggttt gtcgacgtga aggccttcgc gctcgaagtc ggccacgccc catgcttcct
     4021 cgggcaagtc gttgcgctta ccgcaggcga ggagcaacac gacgaggacg gcgatgcgct
     4081 tcatgatggc gtacgctaac gcgggtgagc tggacgtgcc cagactgcaa gcgcagcttc
     4141 ggtcgcaaga accagtcgca tggctgcgca ccgagcggga cggtcgacga ctatttcgcg
     4201 acacggccgg ctgtgctgcg caaaacgttt gacgcggtcg cgcggcacct catgaaacgc
     4261 gacgatgtcc acatcgatgc cgtgaccgtc tgcgtgatgt tcaagcgcga gcgttcgttc
     4321 gccgaggtgc gtgcgaagcg cgaccgactg gacctgtggt tcctgttgtc gcgcactgtc
     4381 gacgatccac gcattcatcg cgcgctgaag ctgtccgcgc atcgcaccgc gcatgtcgtc
     4441 cagctgatgc ggccggccga tgtcgatcgc gacgttcgcg agtggctctc cgaggcctac
     4501 gcctcgtcgc cgacctgagc cgaaacgccc gaaaatggca acggcggaag cccgaaggca
     4561 tccgccgtca ccatcccttt aagttaagcg tgtagcgatt tagagcaggt ccatgccgtc
     4621 gagcagcgac ttcaccgacg cgatgccgtt ctgcgcggcc gccttcgtgg tgtacatctg
     4681 gctgacgccg acaacctggc cgttctcggc cgtgagcgtg aagtagaagc ggttgtccgt
     4741 cgccgtcttg atggcgaacg aaccgtcgag cgccgccgcg tcctggatcg cgaacgcgcc
     4801 gttccacgcc gccgccttcg tggtgtagct ctccgaggag aggacggtct ggccgttctg
     4861 cgcgtggagg ttgaagcgga agccggccgt gctctcgagc acctcgacgc gagcgcccga
     4921 cgtgttcgcc tcgaccttgt cgaggtagct ggtgaccgcg ttgacgcagc tggtgatcgc
     4981 gcgggtcgcg ctcgacttgg tcgagtacgc ctgcgtgaac gcgatgatct cgtagttcgc
     5041 ggcgcggagg cggaggttgt acttgcccga cgccgtcttg ttgacctggt actgcgccgg
     5101 gtcaacgccg ttgtcgagga ccgagaggat gccgttgagc gcgccggtgc gcgacgtgt
//
LOCUS       DASUFX010000846         8074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U1390
            k141_1526435, whole genome shotgun sequence.
ACCESSION   DASUFX010000846 DASUFX010000000
VERSION     DASUFX010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961542
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,814
            CDSs (total)                      :: 6,789
            Genes (coding)                    :: 6,771
            CDSs (with protein)               :: 6,771
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526435"
                     /isolate="SMAG_U1390"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFV99_34020"
     CDS             complement(<1..192)
                     /locus_tag="VFV99_34020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064459.1"
                     /translation="MRRRIAILAAIAAVVATAVVLIVLYARGHRTTTTPVAGRGDGGA
                     AIHAPAGPQLPDQGTRITGV"
     gene            215..679
                     /locus_tag="VFV99_34025"
     CDS             215..679
                     /locus_tag="VFV99_34025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5064460.1"
                     /translation="MGSALFANQRESNLASTVALVEAVLGDLGHDVAQSRLGGDDGLH
                     AWQVRKGSALTRISLVSRTEFTHLRVCSTVMTVDGKVDRAALNAHLLELNAGLCGAAF
                     ASEGDHVLLLAERSTLDLDRAEVRELIKRVTTYADDHDDALVARFGGKMGAG"
     gene            complement(708..971)
                     /locus_tag="VFV99_34030"
     CDS             complement(708..971)
                     /locus_tag="VFV99_34030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064461.1"
                     /translation="MPNYSKLHDYISHRVMIEYDTGAKVVGYLASVQPTSGPVEFVVL
                     SEAKLIDSRGRVVREVGELTMCPNIVTSIAMNEGPSGRDLKAG"
     gene            complement(1000..1329)
                     /locus_tag="VFV99_34035"
     CDS             complement(1000..1329)
                     /locus_tag="VFV99_34035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064462.1"
                     /translation="MLRSLGVLLFIGTVGATGVYFANRPTVARGDVLAADLLQTNPLV
                     QALDCDEKVPLGMTGGRFKCHAEFKNGDSVHFVFAIDRNGIITVAEQGEKTTVPQVKK
                     TSDPWGD"
     gene            1368..2969
                     /locus_tag="VFV99_34040"
     CDS             1368..2969
                     /locus_tag="VFV99_34040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5064463.1"
                     /translation="MSPKPDDGKPKLRKAKFQTDAEIEIKGAYGPDDARGDATPPGSY
                     PFTRGVQPTMYRGQFWTMRQYAGFGTAAESNKRYKYLLAQGSKGLSVAFDLPTQMGRD
                     SDHPMARGEVGRVGVAIDSLEDMRVLFDGIPLGEVSTSMTINATAATLLALYQAVGEE
                     QGVPASALRGTIQNDILKEYIARGTYIYPPAGSMRIITDIFTYCSKDVPQWNTVSISG
                     YHIREAGADAVQEVAFTLADGMAYVKAALDAGLAVDDFASRLSFFFNGHNNFLEEVAK
                     FRAARRMWAKIMRERFGAKSPKSQMLRFHTQTAGVTLQAQQPLVNVPRVTVQALAAVL
                     GGTQSLHTNSYDEALGLPTEEAARIALRTQQVIAHESGVADFIDPLGGSWAVEALTDD
                     IEKRANAYIEEIDKLGGMVPAIERGYPQKEIERRAYEHQRAVEAKQRIVVGVNEYVLE
                     QEEPVPVATIDPELERIQCQRVAALRAKRNAAEHQRALKALDDAAAGTANVLPPIVGA
                     VKAMATVGEIADVMRKRFGEYTPAK"
     gene            complement(2970..4013)
                     /locus_tag="VFV99_34045"
     CDS             complement(2970..4013)
                     /locus_tag="VFV99_34045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5064464.1"
                     /translation="MKVQFRSILFALVLPAAACGGDRETSTTQADPFKWEPKTQAPVS
                     VPVQVGEQPNAVEIPAPLPTKYDEALAKGRELIAAGKLGDAKEMLEQAIKLDKKKAEP
                     HIEMARMYIAMNEKGKSIASAKQAIKLSPLSSQAWNTKGRAELNAHNYDNAIEAFSKA
                     CELNQDNVFAWNNLGYTELLLKKYEDAAEHLGEATSRGGATGYMFNNLGTALEHLDRL
                     DEARTAYDAGGKLGSKEATASRKRLEGVTSIAIAKVDDPAAEAHDEVRGDDGSKGSVK
                     EYDVTDEGDVTDEDRKMEMSGSNSSEEVEGDSTETDSTTDEVDTNVEETVEEPKVEES
                     TVIEPVDAPDDAE"
     gene            complement(4193..4726)
                     /locus_tag="VFV99_34050"
     CDS             complement(4193..4726)
                     /locus_tag="VFV99_34050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064465.1"
                     /translation="MAAVRVIALLAVTACTGGVQSGGGGGSDSGTSGADAKIDAPRLM
                     WVDAASGSGNGLPCENLATPPGDGHHFPGQSCFQSCHNHGFTLAGTLYTNDSGNTAFK
                     GATITVTDATNKTIKIVTNQNGNFYTFDTVQFPILVMASACPSAVKMNDAVAGNNDSR
                     ACNSCHAGGTFNQMHLP"
     gene            complement(4746..5432)
                     /locus_tag="VFV99_34055"
     CDS             complement(4746..5432)
                     /locus_tag="VFV99_34055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5064466.1"
                     /translation="MLALVVPSCGLIDSDVTNFDLTLPDKQFSVDASGWNVNQTAADM
                     FLNMSCSTAPMICSSAATQACPMNCTGACGTTSQKCELQLNVSAYKGIDLVMEKPELN
                     QINDKPVIKVTIDSVKYAVGANTLNVATPEMGVYVAPITVMDPKDPMAKQVGIIEPVP
                     AGATIATRDMTYTSDGKQILIDTMSTYKNPFNVIVGTSKDNPIVIKAGQMVPMGKLDA
                     DIKITAHAGL"
     gene            complement(5584..6528)
                     /gene="meaB"
                     /locus_tag="VFV99_34060"
     CDS             complement(5584..6528)
                     /gene="meaB"
                     /locus_tag="VFV99_34060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5064467.1"
                     /translation="MTTNIEPILKGEVRAAARLMRDLDDRRPDAMDSLKALFPHTGKA
                     YVVGVTGNPGAGKSTVVDALIAYYRAAGERVGVICVDPTSPFSGGAILGDRIRMQRHA
                     LDPDVFIRSLATRGHLGGLSRSTFDVAHVLDAMGYTRILIETVGVGQDEVEVMRAAHT
                     TVVVTVPGLGDDIQAIKAGLLEVADVLVVNKADREGADRTERDLMHMLDLRTGDRKDV
                     EIVRTIATRGTAEGSGIAELANAIEAHRGRAWTGAGAAERAEQRATAQLSELVRALLA
                     DRAARTMAARGGLHEIARSIAEHRQDPWTIAEELVATL"
     gene            complement(6525..7697)
                     /locus_tag="VFV99_34065"
     CDS             complement(6525..7697)
                     /locus_tag="VFV99_34065"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5064468.1"
                     /translation="MRSQRAAVAAALVTVLGACSQPAPKTGPQTPTPATTPKAGSAPA
                     DAPSPSDEEKLAAIQKAMNELDEAAQGCWAMAATERFDIEGELTVLVDITATSAKTQV
                     TTDTARNAKLASCVAQLLAQYKWAPPLYGQSIQLPFKFKAPDGQSVIDRSLVEWKGQG
                     KLSVAVLLDENNTGNPAVSMFELAIGEGGSTGWRKAERTELWYFLHDARVSNPVAVMQ
                     GVRNVKAGDMMFIPAGRVRRVSGAAADMHAVIVVVPGGREGAARAGALPTPESEPSKT
                     SSEILLAVGAKTFGPATIFLDKSIEKGTPLAASILQMPAGIKVPEHVHANETEMLYIL
                     EGSGTMTIAGQDVAVTPTSVIQIPPNTKHAFTATAAVRALQIYTPAGPEQRFKKRP"
     gene            complement(7697..>8074)
                     /locus_tag="VFV99_34070"
     CDS             complement(7697..>8074)
                     /locus_tag="VFV99_34070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX5064469.1"
                     /translation="AKPGLDGHDRGAKVVARALADAGYEVVYTGLHQTPEMIAAAAVQ
                     EAVDAVGLSIMSGAHMTLFPAVIDALKGRGAGDVVVFGGGIVPKDDLPKLEQIGVAKI
                     FTPGASTNEIVDWVETYLRPKVA"
BASE COUNT         1331 a         2673 c         2572 g         1498 t
ORIGIN      
        1 gacgccggtg atgcgcgtgc cctgatccgg caattgcggg cccgctggcg cgtggatcgc
       61 cgcaccgccg tcgccgcggc ccgcgaccgg agtcgtcgtc gtgcggtgcc cccgtgcgta
      121 gaggacgatc aacaccacgg ccgtcgcgac gaccgcggca attgccgcca ggatcgcgat
      181 gcgtcttcgc acagcgggat ggtagtctga cctcgtgggg agcgcactgt ttgccaatca
      241 gcgagagtcg aacctggcgt ccaccgtcgc gctcgtcgaa gcagtgctcg gcgatctcgg
      301 tcacgatgtc gcgcaaagcc ggctcggcgg cgatgacggg ctacacgcgt ggcaggtccg
      361 caagggctcg gcgctgactc ggatctcgct cgtcagccgc accgagttca cgcacctgcg
      421 cgtgtgctcg acggtgatga ccgtcgacgg caaggtcgat cgcgcggcgc tcaacgcaca
      481 cttgctcgag ctcaatgcag gcctgtgcgg cgcggcgttc gcgtccgaag gcgatcacgt
      541 gctgctgctc gccgaacgct cgacgctcga tctcgatcgc gccgaagtac gcgagctgat
      601 caaacgcgtg acgacgtacg ccgacgacca cgacgacgcg ctcgtcgccc gcttcggcgg
      661 caagatgggc gcgggctgat cgaccgccta ggcgacgcct aggacgatca cccagctttg
      721 agatcgcggc cgctcgggcc ttcgttcatc gcgatcgagg tcacgatgtt cgggcacatc
      781 gtcagctcgc cgacctcgcg gaccacgcgg ccacgcgaat cgatgagctt ggcttcggag
      841 agcaccacga actcgaccgg gcccgaggtg ggctggaccg acgcgagata gccgacgacc
      901 ttcgcaccgg tgtcgtattc gatcatgacg cgatggctga tgtagtcatg cagcttcgag
      961 taattgggca tcgcaggccc ctagataaca caatcgcgtt cagtctcccc acggatcgga
     1021 cgtcttcttc acctggggaa cggtggtctt ctcgccctgc tcggcgacgg tgatgatgcc
     1081 gttgcggtcg atcgcgaaca cgaagtgcac gctgtcgccg ttcttgaatt cagcgtggca
     1141 cttgaaccgg cctcccgtca tgccgagcgg gactttctcg tcgcaatcga gcgcttggac
     1201 gagcggattc gtttgcagga gatcggccgc cagcacgtct ccacgcgcaa cggtgggccg
     1261 gttggcgaag taaacgcccg tggccccgac agtgccgatg aacagaagaa cgcccagcga
     1321 ccgcagcacc gcatcattgt agccgatctg atgctacaca cggcttcgtg agcccgaagc
     1381 ccgatgatgg gaagccgaag ttgcggaagg ccaagttcca gaccgacgcc gagatcgaga
     1441 tcaaaggcgc ctacggtccc gacgatgcgc gcggcgacgc gacgccgccc ggcagctacc
     1501 cgttcacgcg cggcgtgcag ccgacgatgt atcgcggcca gttctggacg atgcgccagt
     1561 acgcaggctt tggcaccgcg gccgaatcga acaagcgcta caagtatctg ctcgcgcaag
     1621 gcagcaaggg tctgtcggtc gcgttcgacc tgccgaccca gatgggccgc gacagcgatc
     1681 atccgatggc gcgtggcgag gtcggccgcg ttggcgtcgc gatcgattcg ctcgaggaca
     1741 tgcgcgttct gttcgacggc attccgctcg gcgaagtctc gacgtcgatg acgatcaacg
     1801 cgaccgcggc gacgctgctc gcgctgtatc aagcggtcgg cgaggaacaa ggcgtgccgg
     1861 cgagcgcact gcgcggcacg atccagaacg atatcctcaa ggagtacatc gcgcgcggca
     1921 cgtacatcta tccgccggcc ggctcgatgc gcatcatcac ggacattttc acgtactgca
     1981 gcaaggacgt gccgcagtgg aacacggtgt cgatcagcgg ctatcacatc cgtgaagccg
     2041 gcgccgacgc cgtgcaggag gtcgcgttca cgctcgccga cggtatggcg tacgtgaagg
     2101 ccgcgctcga tgccgggctc gccgtcgacg atttcgcgtc gcgcctgtcg ttcttcttca
     2161 acgggcacaa caacttcctc gaggaagtgg cgaagttccg cgccgcccgc cgcatgtggg
     2221 ccaagatcat gcgcgagcgc ttcggcgcga agagcccgaa gagtcagatg ctgcgcttcc
     2281 acacgcagac tgctggcgtc acgctgcagg cgcagcagcc gctcgtcaac gtaccgcgcg
     2341 tgaccgtgca ggcgctcgcc gcggtgctcg gcggcacgca gtcgctgcac acgaacagct
     2401 acgacgaggc gctcggcctg cccaccgagg aagccgcacg catcgcgctg cgcacacagc
     2461 aagtcatcgc gcacgagagc ggcgttgccg acttcatcga tccgctcggc ggcagctggg
     2521 cagtcgaagc gctgaccgac gacatcgaga agcgcgcgaa cgcgtacatc gaagagatcg
     2581 acaagctcgg cggcatggtg ccagcgatcg agcgcggcta tccgcagaag gaaatcgagc
     2641 ggcgcgcgta cgagcaccaa cgtgccgtcg aggcaaagca gcgcatcgtg gttggcgtca
     2701 acgagtacgt gctcgaacaa gaggagccag tgcccgtcgc gacgatcgat cccgagctcg
     2761 agcgcatcca gtgtcagcgc gtcgccgcgc tccgcgcgaa acgcaacgcg gccgaacacc
     2821 agcgcgcgct caaggcgctc gatgacgcag ccgcgggaac ggcgaacgtg ctaccgccga
     2881 tcgtgggtgc cgtgaaggcg atggcgaccg tcggcgaaat cgccgacgtc atgcgcaagc
     2941 gcttcggcga gtacacgccg gcaaagtgat cactccgcgt cgtcgggtgc gtcgaccggc
     3001 tcgatgacgg tcgattcctc gaccttgggc tcctcgaccg tctcttcgac gttcgtgtcg
     3061 acctcgtcgg tcgtgctgtc agtctcggtg ctgtcgccct cgacctcttc actgctgttg
     3121 ctgcccgaca tctccatctt gcggtcttcg tcggtgacgt cgccctcgtc ggtgacgtcg
     3181 tactccttca ccgagccctt cgagccatcg tcgccccgaa cttcgtcgtg agcttccgcc
     3241 gccgggtcgt cgaccttggc gatcgcgatc gaggtcacgc cttcgaggcg cttgcggctt
     3301 gcggtcgctt ccttcgaacc gagcttgccg ccagcgtcgt atgccgtacg ggcctcgtcg
     3361 agacgatcga gatgctcgag tgcggtgccg aggttgttga acatgtagcc ggtggcgccg
     3421 ccacggctcg tcgcctcgcc caggtgctcg gcggcgtcct cgtacttctt gaggagcagc
     3481 tcggtgtaac cgaggttgtt ccaggcgaac acgttgtcct ggttgagctc gcacgccttc
     3541 gagaacgctt cgatcgcgtt gtcgtagttg tgcgcgttga gctcggcgcg gcccttcgtg
     3601 ttccacgcct ggctcgacag cggcgagagc ttgatcgcct gcttcgcgct cgcgatcgac
     3661 ttgcccttct cgttcatcgc gatgtacatg cgcgccatct cgatgtgcgg ctcggctttc
     3721 ttcttgtcga gcttgatcgc ctgctcgagc atttcctttg cgtcaccgag cttgccggcc
     3781 gcgatcagct cgcgaccctt cgcgagtgct tcgtcgtact tcgtcggcag cggagccgga
     3841 atctcgacgg cgttgggctg ctcgccgact tgcacgggaa cgctgaccgg agcctgcgtc
     3901 tttggctccc acttgaatgg atcggcttgc gtcgtcgatg tctcgcgatc accgccgcac
     3961 gccgccgccg gaagaacgag agcaaacagg atggatcgga actggacctt catagctgcc
     4021 tctaaccctt tcgctctctc ccttcagcat ccgacgtgcc atcggaagcg atcaggcgcc
     4081 aagccaactt gtgcgcagat gtgggtacgc agacacgccg atcgtcgacg ggcgcacgcg
     4141 tggaatggaa tcggcgtcga cggcgttcta gaaggccgcc tcgacgagac cgctacggca
     4201 ggtgcatctg gttgaacgta ccgccggcgt ggcacgagtt gcacgcgcgg ctgtcgttgt
     4261 tgcccgccac ggcgtcgttc atcttcaccg ccgacggaca tgcgctcgcc atcacgagaa
     4321 tcgggaactg cacggtgtcg aacgtgtaga agttgccgtt ctggttcgtg acgatcttga
     4381 tcgtcttgtt cgtcgcgtcg gtcaccgtga tcgtcgcgcc tttgaacgca gtgttgcctg
     4441 agtcgttcgt gtagagcgtg ccggcgagcg tgaagccatg gttgtggcag gactggaagc
     4501 agctctggcc gggaaagtgg tgaccatcgc ccggcggcgt cgcgaggttt tcgcacggca
     4561 ggccgttgcc gctgccgctc gcggcgtcga cccacatcag gcgtggcgca tcgatcttcg
     4621 cgtccgcacc ggatgtgccg ctgtcactgc cgccaccgcc gccactctgc acgccacctg
     4681 tgcacgcagt gactgcgaga agcgcgatga cacggactgc cgccatcgac gcgatggtaa
     4741 accgactaca gaccggcgtg cgccgtaatc ttgatgtctg cgtctaactt gcccatcggc
     4801 accatctggc ccgccttgat gacgatcggg ttgtccttcg acgtgccgac gatgacgtta
     4861 aacggattct tgtaggtgct catcgtgtcg atgaggatct gcttgccgtc gctcgtatac
     4921 gtcatgtcgc gcgtcgcgat cgtcgcgccc gcggggaccg gctcgatgat cccgacctgc
     4981 ttcgccatcg gatccttcgg atccatgacg gtgatcggcg cgacgtaaac gcccatctcc
     5041 ggcgtcgcga cgttcagcgt gttcgcgccg accgcgtact tcacgctgtc gatcgtcacc
     5101 ttgatgacgg gcttgtcatt gatctggttg agctcgggct tctccatcac gaggtcgatg
     5161 cccttgtacg cgctcacgtt gagctggagc tcgcacttct ggctcgtcgt gccgcaagcg
     5221 cccgtgcagt tcatcgggca cgcttgcgtc gctgccgacg aacagatcat cggagcggtc
     5281 gagcagctca tgttgaggaa catgtcggct gccgtctggt tgacgttcca gcccgacgcg
     5341 tcgacgctga actgcttatc cggcagcgtc agatcaaaat tcgtgacgtc agaatcgatc
     5401 aagccgcagc tcggaacgac caacgccaac aagcacaaac gaagataggt cataaggaat
     5461 gcctccaaaa ccccgaggca aaaccagtct accccgtcgg aacgcgggtg gcccagaaac
     5521 cggcatcccc acgtgatagg cgttacggct tgtaccagcg ctgcacgctg tagcggacaa
     5581 cacctacagt gtggctacga gctcttcggc aatcgtccac ggatcttgcc ggtgctcggc
     5641 gatggaccgc gcgatttcgt gcagcccacc gcgcgcagcc atcgtacgag cggcgcggtc
     5701 tgcgaggagc gcacgcacta gctcgctaag ctgcgcggta gcacgttgtt ctgcgcgttc
     5761 agcagcaccc gcccctgtcc aggcgcggcc gcgatgcgcc tcgatcgcgt tcgcgagctc
     5821 ggcgatgcca gatccttccg ccgtgccacg tgtcgcgatc gtcctcacga tctccacgtc
     5881 tttccgatct ccggtccgca gatcgagcat gtgcatgagg tcacgctccg tacggtcggc
     5941 gccttcgcga tcggccttgt tgacgacgag tacgtcggcg acttctagca ggccggcctt
     6001 gatcgcctgg atgtcatcgc cgagaccagg caccgtcacg acgactgtcg tgtgcgcggc
     6061 gcgcatcacc tcgacctcgt cctgtccgac gccgaccgtt tcgatcagga tgcgcgtata
     6121 tcccatcgcg tcgagtacgt gcgcgacatc gaacgtcgag cgtgacaggc caccgagatg
     6181 gccgcgcgtc gcgaggctgc ggatgaatac gtccggatcg agcgcgtggc gctgcatacg
     6241 gatgcggtcg ccgaggatcg cgccgcccga gaacggcgat gtcggatcga cgcagatcac
     6301 gccgacacgt tcgccggctg cacggtagta cgcgatcaac gcatcgacga cggtcgactt
     6361 gccagcgccc ggattgccgg tcacaccgac gacgtacgcc ttgccggtat gcgggaacag
     6421 cgccttgagc gagtccatcg cgtcgggcct gcggtcgtcg aggtcgcgca tcaaccgggc
     6481 ggcggcgcgg acttcacctt tcagaatcgg ctcgatgttc gtcgtcatgg tcgtttcttg
     6541 aagcgctgct cggggccggc gggcgtgtag atctgtagcg cgcgaacggc cgctgtggcg
     6601 gtgaacgcgt gctttgtatt cggcggaatc tgaatcaccg acgtcggcgt caccgcgaca
     6661 tcctgacccg cgatcgtcat cgtgccgctg ccctcgagga tgtacagcat ctcggtctcg
     6721 tttgcgtgca cgtgctctgg caccttgatg ccggctggca tctgcaggat cgacgcggcg
     6781 agcggtgtgc ccttctcgat cgatttgtcg aggaagatcg tcgccggacc gaacgtcttc
     6841 gcgccgaccg cgagcaggat ctccgacgac gtcttgctcg gttccgactc aggcgtcggc
     6901 agcgcccccg cacgtgccgc accttcccgg ccaccaggca cgacaacgat caccgcgtgc
     6961 atgtccgccg cggcaccgct cactcggcgc accctgccgg ccgggatgaa catcatgtcg
     7021 ccggccttca cgttgcgcac accctgcatc accgcgaccg ggttactgac gcgcgcatcg
     7081 tggagaaaat accagagctc ggtgcgttcg gctttgcgcc agccggtact gccgccctct
     7141 ccaattgcga gctcgaacat cgacactgca ggattgccgg tgttgttctc gtcgagcagc
     7201 accgcgaccg acagcttgcc ctgccccttc cactcgacga gtgaacgatc gatcacgctc
     7261 tggccgtcgg gtgccttgaa cttgaacggc agctggatcg actgcccgta tagcggcggt
     7321 gcccacttgt actgggcgag cagctgggcg acgcagctcg cgagcttcgc gttgcgcgcg
     7381 gtgtcagtcg tgacctgggt cttcgcgctg gtcgcggtga tgtcgacgag aaccgtgagc
     7441 tcaccttcga tgtcgaaccg ctcggtcgcg gccattgccc agcagccctg cgcggcctcg
     7501 tcgagctcgt tcatcgcctt ctggatcgcc gcgagcttct cctcgtcgct cggcgacggt
     7561 gcatcggcag gcgcactccc cgccttcggc gtcgtcgctg gcgttggcgt ttgcggcccg
     7621 gtcttcggtg caggctgact gcacgccccg agcaccgtaa ccagcgctgc ggcaaccgcc
     7681 gcacgctgcg agcgcatcac gccaccttcg gccgcaggta cgtctcgacc cagtcgacga
     7741 tttcgttcgt gctcgcgccg ggcgtgaaga tcttcgcgac gccgatctgc tcgagctttg
     7801 gcaggtcgtc cttcggcacg atgccgccgc cgaacacgac gacgtcgccg gcgccgcggc
     7861 ccttgagcgc gtcgatcacg gcggggaaca gcgtcatgtg cgcgccggac atgatcgaca
     7921 ggccgactgc gtcgacggct tcctgcaccg cggcggccgc gatcatctcg ggtgtctggt
     7981 gcagaccggt gtagacgact tcgtagccgg cgtcggcgag cgcacgagcg acgacttttg
     8041 cgccgcgatc atgaccgtcg agacctggct tggc
//
